LOCUS       DASVOR010000001         7261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_87681,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000001 DASVOR010000000
VERSION     DASVOR010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87681"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF516_00005"
     CDS             <1..241
                     /locus_tag="VF516_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106068.1"
                     /translation="RNQWKRERIAPSFHLDDRNVDPRSWCRFPILSGGFERELAELRA
                     YVASGKADPAGGPAGGPAGGPAGGPAGGPAGGPDR"
     gene            238..927
                     /locus_tag="VF516_00010"
     CDS             238..927
                     /locus_tag="VF516_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8106069.1"
                     /translation="MSHAYEYPRAALAVDCAVFGLDEADLKVLLIQRKLPPFQHAWAL
                     PGGFVRLDETLDEAAQRELREEAGVEDVYLEQLYTFGALGRDPRERVVTVAYYALAKL
                     SDHRIRAATDAMGVGWFALDDLPKLAFDHEHIVARARERLRGKVRYAPVGFELLPPRF
                     SLTQLQRLYEIILGTELDKRNFRKKILSMDLLVETDEVEQGVRHRAARLYRFDRRKYE
                     RLLKQGFELSI"
     gene            937..1554
                     /gene="pncA"
                     /locus_tag="VF516_00015"
     CDS             937..1554
                     /gene="pncA"
                     /locus_tag="VF516_00015"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF008623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HEX8106070.1"
                     /translation="MRALILVDLQNDFCPGGALAVARGDETVAIANRLLPYFSIVVAT
                     QDWHPADHESFAPQHRGRKPGEMIELHGLPQVLWPPHCVQGTRGADLHPGLQRDKITE
                     VFHKGTDRTVDSYSGFFDNGHRKSTGLADWLRERWITRVYVMGLATDYCVKATALDAR
                     ELGFDTWVIEDGCRPVELHPGDGDRALGAMRAAGATIVESGSIGS"
     gene            complement(1610..3082)
                     /locus_tag="VF516_00020"
     CDS             complement(1610..3082)
                     /locus_tag="VF516_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEX8106071.1"
                     /translation="MSKQATTLVLGAILSTLAMTACNLDIPDLNNPGIEQLENNPTAP
                     GINTALTGMLIGDRTAKATTAGLVCQLGILGREAYDIDPAEGRPVTELLGGKLNPSSP
                     FGGAFRAPPYANIRLGNIVLHALDKVSAFTDPQKAGIRGFVHTIMAMELLTVILTRDD
                     IGAPIDTDRDLSAPLAPFVPKAEVYKKITDLLDQAQGELMMATAGAPDPTKVTFTHTL
                     SPGYTGFNTPVTFLLFNRALRAKVAVYLKDYPTAMKIFTAPAGSPDKTFLNDASTVTF
                     TNKPGDDKGVFHVYSTTTGDATNGLINPVVFAHPSLTDDVAKDMSTPNPKPIDARYTA
                     KVMDTGSTTTAGGDQAVKTSLVFLVYPSNASPVPVIRNEELILLEAEAMWFTGNQSGA
                     VDKLNLVRTKAGKLTALDPATIMDDTGFINALLYERRFSLMFEGGHRWIDLRRFGRSL
                     LTAGETTHTQNLRYPIPQPECDARPGEPRCMLNSVDPITN"
     gene            complement(3097..6582)
                     /locus_tag="VF516_00025"
     CDS             complement(3097..6582)
                     /locus_tag="VF516_00025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEX8106072.1"
                     /translation="MLLFSFAARAQPAKPGKGRDIAGSVLDVDGNPVAGATVTVSDGG
                     PSATTAADGSFKLTGVATTNVAIAITADGFTAKQVPVLGAATALELQVVLVRPTPAAP
                     PPVETRMIGGVVSDAAHAPIAGATVRVHGSQIQGVTAGDGSFTLPGVALGEVTIDVEA
                     PNQPPASVVVPADRAAVVVTVGAAVAPAPPKTRSVRGKVTEASGEPLAAAQVQVAGIP
                     EATVFTETDGTFAFENLPAGKITLDITAPEHETQVLEVAPDKDAVAIKLGLATGEQIV
                     IEGRAPSITKENITGGGSVIKGSDLTRVSAATLDDAMVGKLAGANLQANSGAPGGGAQ
                     LRLRGISTINGQNSPLYVIDGVIISNINTSSGINAVTAASGNGGLPSNQDNPVNRVAD
                     LNPNDIENVEVLKGASAAALYGSKAANGVVIITTKHGKLGENHASVTQRIGFSQASKT
                     YGNRTWGSVDEVKGFYCASTDTPDKCNANPYVLAYVAANGQTFNHEAEITRTPFLMET
                     LADVTGGTEAGNYYGSLLLTDQPAVMKGTFYQKQSGRVAVGYKFYDRVKLSLTTNVLH
                     SDSDRGVTNNDNTGTSEYFVLSATPNFVDLRPVNGVYPANLGKNLHANPLQTVAELQN
                     HEELTRLISGGTATVDAYASPDNEHRVLLRGNVGIDMFTQKNHVLSPNDLTFENDDKL
                     PGTVVDGTTNNTNFNLGTSAVWNYTPHTGGLRSAFTGGLTFESVNTHTVSSLARNLTA
                     GELKVDTASFLRASENNLQTKEVGGYVQEEVALLDSRLSVLGGLLSERSSLNGDASKY
                     YFYPKLGAAYSLIRPAKPGEAPLFQGFESLRVRVNYGETGNRPNYGNKFTALNAVNTI
                     AGTAGIVVGASAGDPSISPERQREFEGGIDAATKDQRVVAEVTVYQRNISNMLLQQAL
                     ATTTGFTTQFTNGGGMRNRGVEAAVAVKPVPGFDWTSRGTLTLNRSEVTDLPANIPAF
                     LVPVGFGASLGAYQIKPGQSATAIVATVNGTLQLVGDGEPDFRVGWSNVVNWGDFAFA
                     TLLDWQHGSKVIDLTTQAYDQNGNAPDQAAAAKRRAILMSGDPRPYIEDGSFVKVREI
                     SVAYNLPKRLVTQLGPVKSLQVSASGRNLLTFSSYLGLDPEVSNFGNQSIARNYDVTP
                     YPPSRTFWLSVTAGI"
     gene            7027..>7261
                     /locus_tag="VF516_00030"
     CDS             7027..>7261
                     /locus_tag="VF516_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106073.1"
                     /translation="MKLDKPTQDGNQGEGDRISGRRYQRNVREFVATGQVEPAARDAA
                     RYVERAPDDAARAEAAARRGPRPTRVSLDELVAK"
BASE COUNT          999 a         2368 c         2655 g         1239 t
ORIGIN      
        1 caggaaccag tggaagcgcg agcggatcgc gccgagcttt cacctcgacg accgcaacgt
       61 cgacccgcgg tcgtggtgcc ggttcccgat cctgtcgggc gggttcgagc gcgagctcgc
      121 cgagctgcgc gcctacgtcg cgtcggggaa ggccgatccc gccggggggc ccgccggggg
      181 gcctgccggg gggcctgccg gggggcctgc cggggggcct gccgggggcc ctgatcgatg
      241 agccacgcct acgaataccc gcgggcggcg ctcgcggtcg actgcgcggt gttcgggctc
      301 gacgaggccg acctcaaggt cctcttgatc cagcgcaagc tgccgccgtt ccagcacgcc
      361 tgggcgctgc ccggcgggtt cgtccggctc gacgagacgc tcgacgaggc ggcccagcgc
      421 gagctccgcg aggaggccgg ggtcgaggac gtctacctcg agcagctcta cacgttcggc
      481 gcgctcggcc gcgacccgcg cgagcgcgtg gtcaccgtgg cgtactacgc gctcgccaag
      541 ctcagcgatc accggatccg cgcggcgacc gacgcgatgg gggtcggctg gttcgcgctc
      601 gacgacctgc ccaagctcgc gttcgatcac gagcacatcg tggcgcgcgc gcgcgagcgg
      661 ctgcgcggca aggtgcgcta cgcgccggtc gggttcgagc tgttgccgcc gcggttctcg
      721 ctgacccagc tccagcggct gtacgagatc atcctcggaa ccgagctcga taaacgcaac
      781 ttccgaaaga agatcctatc gatggacctg ctcgtcgaga ccgacgaggt ggagcagggt
      841 gtacgacatc gcgccgcgcg gctgtaccgc ttcgaccgcc gcaagtacga gcggctgctc
      901 aagcaaggct tcgagctgtc gatctaagat acccacatgc gagcgttgat cctggtcgac
      961 ctgcagaacg acttctgtcc cggcggtgcg ctcgccgtcg cgcgcgggga cgagacggtc
     1021 gccatcgcca accggctgtt gccgtacttc tcgatcgtgg tcgccaccca ggactggcac
     1081 cccgcggacc acgagagctt cgcgccgcag caccgcggcc gcaagccggg cgagatgatc
     1141 gagctccacg gcctgcccca ggtgctgtgg ccgccgcact gcgtgcaggg cacccgcggc
     1201 gccgatctcc accccggcct ccagcgggac aagatcaccg aggtgttcca caaggggacc
     1261 gatcgcacgg tcgacagcta cagcgggttc ttcgacaacg gccaccgcaa gtcgaccggc
     1321 ctggccgact ggctgcgcga gcgctggatc acccgggtct acgtcatggg cctggcgacc
     1381 gactactgcg tcaaggccac cgcgctcgat gcccgcgagc tcgggttcga cacctgggtg
     1441 atcgaggacg gctgccggcc ggtcgagctc caccccggcg acggcgaccg cgcgctcggc
     1501 gcgatgcgcg cggccggcgc cacgatcgtc gagagcggct cgatcggctc gtgacgggag
     1561 gcccgtgacg ggcggcccgt gacgggcggc cgcgcggccg gtcacccgat cagttcgtga
     1621 tcggatccac gctgttgagc atgcaccgcg gctcgccggg ccgcgcgtcg cactcgggct
     1681 gcgggatcgg gtagcggagg ttctgcgtgt gcgtggtctc gccggccgtc agcagcgagc
     1741 ggccgaaccg acggaggtcg atccagcggt ggccgccctc gaacatcagc gagaaccggc
     1801 gctcgtagag cagcgcgttg atgaacccgg tgtcgtccat gatcgtcgcg gggtcgagcg
     1861 cggtcagctt gccggccttg gtgcgcacga ggttgagctt gtcgactgcc ccggattgat
     1921 tgccggtgaa ccacatcgcc tcggcctcga gcaggatcag ctcctcgttg cggatcaccg
     1981 gcaccggcga cgcgttgctc ggatagacca ggaacacgag gctggtcttg acggcctggt
     2041 cgccgcccgc cgtggtcgtg ctgccggtat ccatgacctt ggccgtgtag cgtgcgtcga
     2101 tgggcttggg gttgggggtg gacatgtcct tcgcgacgtc gtccgtgagc gacgggtggg
     2161 cgaacaccac ggggttgatc agcccgttgg tcgcgtcgcc ggtggtcgtc gagtagacgt
     2221 ggaacacgcc cttgtcgtcg ccgggcttgt tggtgaacgt gaccgtggag gcgtcgttca
     2281 ggaacgtctt gtccggtgag cctgcgggcg cggtgaagat cttcatcgcg gtcgggtagt
     2341 ccttgaggta cacggcgacc ttcgcccgga gcgcgcggtt gaacaggagg aacgtcaccg
     2401 gcgtgttgaa gcccgtgtag cccggcgaca gcgtgtgcgt gaacgtgacc ttggtcggat
     2461 ccggcgcgcc ggcggtcgcc atcatgagct cgccctgcgc ctgatcgagc aggtcggtga
     2521 tcttcttgta gacctcggcc ttggggacga acggcgcgag cggggcgctc agatcgcgat
     2581 cggtgtcgat cggcgcgccg atgtcgtcgc gggtcaggat caccgtgagc agctccatcg
     2641 ccatgatcgt gtggacgaag ccgcggatcc ccgccttctg cggatcggtg aaggccgaga
     2701 ccttgtcgag cgcgtgcagg acgatgttgc cgagccggat gttggcgtac gggggggccc
     2761 tgaatgcgcc gccgaacggg ctcgacgggt tgagcttgcc accgagcagc tcggtgaccg
     2821 gccggccctc ggcggggtcg atgtcgtagg cctcgcggcc caggatgccg agctggcaca
     2881 ccagcccggc ggtggtcgcc ttggcggtac ggtcgccgat cagcatgccg gtcagggcgg
     2941 tgttgatgcc gggcgcggtc ggattgttct cgagctgttc gatgcccggg ttgttgagat
     3001 cggggatgtc gaggttgcag gccgtcatgg ccagcgtgct caggatcgcg ccgagcacga
     3061 gcgtcgttgc ttgcttggac atggcttcgc tcctcctcag atgcccgccg tgacggagag
     3121 ccagaacgtc cggctcggcg gatagggggt cacgtcgtag ttgcgggcga tcgactggtt
     3181 gccgaagttc gagacctcgg gatccaggcc gaggtagctg ctgaacgtca gcaggttgcg
     3241 tccgctcgca ctgacctgga ggctcttgac cgggccgagc tgcgtcacca ggcgcttggg
     3301 caggttgtag gcgaccgaga tctcgcgcac cttcacgaac gagccatcct cgatgtacgg
     3361 gcgcggatcg ccgctcatga ggatcgcccg gcgcttggcg gcggccgcct ggtcgggcgc
     3421 gttgccgttc tggtcgtagg cctgcgtggt caggtcgatg accttcgagc cgtgctgcca
     3481 gtcgagcagc gtggcgaacg cgaagtcgcc ccagttgacg acgttcgacc agccgacccg
     3541 gaagtcgggc tcgccgtcgc cgaccagctg gagggtcccg ttgaccgtcg cgacgatcgc
     3601 ggtcgcgctc tggccgggct tgatctggta cgcgccgagg ctggcgccga acccgaccgg
     3661 caccaggaac gccgggatgt tggccggcag gtcggtgacc tccgagcggt tgagcgtcag
     3721 cgtgccgcgc gaggtccagt cgaagcccgg gaccggcttg accgcgaccg ccgcctcgac
     3781 gccgcggttg cgcatcccac cgccgttggt gaactgcgtg gtgaacccgg tcgtggtcgc
     3841 cagcgcctgc tgcagcagca tgttcgagat gttgcgctgg tacacggtga cctcggcgac
     3901 cacgcgctgg tccttggtcg ccgcgtcgat gccgccctcg aactcgcgct ggcgctcggg
     3961 cgagatgctg ggatcgccgg cgctggcgcc gacgacgatc ccggcggtgc cggcgatcgt
     4021 gttgaccgcg ttgagcgccg tgaacttgtt gccgtagttc ggccggttgc cggtctcgcc
     4081 gtagttcacg cggacgcgca gcgactcgaa gccctggaac agcggcgcct cgcccggctt
     4141 ggccggccgg atcagcgagt acgccgcgcc gagcttggga tagaagtagt acttgctggc
     4201 gtcgccgttg aggctcgagc gctcggacag gaggccgccg agcaccgaca gccggctgtc
     4261 gagcagcgcc acctcctcct ggacgtagcc gccgacctcc ttggtctgca ggttgttctc
     4321 gctggcccgg aggaagctcg cggtgtcgac cttgagctcg ccggcggtga ggttgcgggc
     4381 gagcgaggac accgtgtggg tgttgacgct ctcgaaggtc aggccaccgg tgaacgcgct
     4441 gcgcagcccg ccggtgtggg gcgtgtagtt ccacaccgcg ctggtcccga ggttgaagtt
     4501 ggtgttgttg gtcgtgccgt cgaccacggt cccgggcagc ttgtcatcgt tctcgaacgt
     4561 caggtcgttc ggcgacagca cgtggttctt ctgggtgaac atgtcgatcc cgacgttgcc
     4621 gcgcagcagg acccggtgct cgttgtcggg gctcgcgtac gcgtcgaccg tggcggtccc
     4681 gccggagatc agacgggtca gctcctcgtg gttctggagc tcggcgacgg tctgcagcgg
     4741 gttggcgtgc aggttcttgc ccaggttggc cgggtacacg ccgttcaccg ggcggaggtc
     4801 gacgaagttg ggcgtggcgc tgagcacgaa gtactcggac gtgccggtgt tgtcgttgtt
     4861 ggtcacgccg cggtccgagt ccgagtgcag cacgttggtc gtgagcgaca gcttgacgcg
     4921 atcgtagaac ttgtatccga ccgcgacccg gccgctctgc ttctggtaga acgtcccctt
     4981 catcaccgcg ggctgatcgg tgagcaggag cgagccgtag tagttgccgg cctcggtgcc
     5041 gccggtgacg tcggcgaggg tctccatgag gaacggcgtg cgcgtgatct cggcctcgtg
     5101 gttgaacgtc tggccgttcg cggcgacgta ggccaggacg taggggttgg cgttgcactt
     5161 gtcgggcgtg tcggtcgagg cgcagtagaa gcccttgacc tcgtcgaccg agccccacgt
     5221 gcgattgccg taggtcttcg aggcctggga gaacccgatc cgctgggtca ccgaggcgtg
     5281 gttctcgccc agcttgccgt gcttggtggt gatgatgacc acgccgttgg ccgccttgga
     5341 gccgtacagc gcggcggccg aggcgccctt gagcacctcg acgttctcga tgtcgttggg
     5401 gttgaggtcg gcgacccggt tgaccgggtt gtcctggttc gacggcaggc cgccgttgcc
     5461 cgaggccgcg gtcaccgcgt tgatgccgga cgacgtgttg atgttcgaga tgatcacgcc
     5521 gtcgatcacg tagagcggtg agttctggcc gttgatcgtc gagatcccgc gcagccggag
     5581 ctgggcgccg ccgccgggcg cgcccgagtt ggcctgcagg ttggcgccgg cgagcttgcc
     5641 gaccatcgcg tcgtcgagcg tcgcggccga cacccgggtc aggtcggagc ccttgatcac
     5701 cgagccgccg ccggtgatgt tctccttggt gatcgacggc gcgcggccct cgatcacgat
     5761 ctgctcgccg gtcgcgagcc cgagcttgat cgcgaccgcg tccttgtcag gggcgacctc
     5821 gagcacctgg gtctcgtgct ccggcgcggt gatgtcgagc gtgatcttgc cggccggcag
     5881 gttctcgaac gcgaacgtgc cgtcggtctc ggtgaacacc gtcgcctcgg ggatgccggc
     5941 gacctggacc tgggcggccg cgagcggctc ccccgaggcc tcggtcacct tgccgcggac
     6001 cgagcgcgtc ttgggcggcg ccggtgccac cgccgcgccg accgtcacca cgaccgcggc
     6061 cctgtccgcg ggcacgacca cgctggccgg cggctggttg ggcgcctcga cgtcgatcgt
     6121 gacctcgccg agcgcgacgc cgggcagcgt gaagctgccg tcgccggcgg tgacgccctg
     6181 gatctgcgag ccgtggacgc gcaccgtggc gcccgcgatc ggcgcgtgtg ccgcgtcgct
     6241 gaccacgccg ccgatcatgc gggtctcgac cgggggtggc gccgccgggg tcggcctgac
     6301 cagcacgacc tggagctcca gcgcggtcgc cgcgccgagc accgggacct gcttggccgt
     6361 gaagccgtcc gcggtgatcg cgatcgccac gttggtggtg gcgaccccgg tcagcttgaa
     6421 gctgccgtcg gcggccgtgg tcgccgatgg gcctccgtcg ctgactgtca ccgtggcgcc
     6481 ggcgaccggg ttgccgtcaa cgtccaggac gctgccggcg atgtcgcggc ccttgcccgg
     6541 cttggcgggc tgtgcgcgcg cagcgaagga gaacagtaac aatgcggtga agcagagtgc
     6601 gggtccgagt aacttcgcaa gtcgctttgt gatcatcacg gaggcccccc taggtgattc
     6661 gcgccatcta aacgccgcgt gatgtcggct acaaggaaat gaaccgacac aatctgtctg
     6721 ctggcgcgag ttcctggtcg cagtgctgtc gcggcccccc tgcgtgtaac ggaccctaca
     6781 taatttcgat cgcttagctg gttgcttcgc cgcatccatc tgtgccgcat gcgtcgcagg
     6841 acagccccgg tgttccggtc gggcggatct ccgtggccga ggggaatatt tctcggagag
     6901 cgcatcggcc gcgcgagcga ggcaatcgcg cgcggcgatg cagcatcgcc gtcaccccct
     6961 cgtctccgag atcgtcgggc ggcgcgatca ccgagccgtg gcacgcggtg tgctgcctct
     7021 ccggacatga agctcgacaa gcccacccaa gatggaaatc aaggagaagg cgatcggatc
     7081 tcggggcgcc gctatcagcg gaacgttcgc gagtttgtcg caaccggcca ggtcgagcct
     7141 gcggcgcgcg acgccgcgcg ctacgtggag cgagcgcccg acgatgcggc gcgtgccgag
     7201 gcggcggcgc ggcgcgggcc caggccgacg cgggtctcgc tcgacgagct cgtggccaag
     7261 g
//
LOCUS       DASVOR010000002         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_116925,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000002 DASVOR010000000
VERSION     DASVOR010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116925"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VF516_00035"
     CDS             <1..994
                     /locus_tag="VF516_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX8106074.1"
                     /translation="EATQVSMQGQGYAWTPGIHSREQVEGWRVITDAVHDAGGRMFIQ
                     LWHVGRISHPALQPDHMLPVAPSPITPAGKAFIENELGEGELVPFVRPRGLELEELPY
                     VARQYARGARNAQLANADGVEVHGANGYLLEQFLETGTNRRGDAYGGSVAHRARMLLD
                     VVETCIAVWGPERVGVRISPLSTFNDIHDDNPEETFAYIAEQLSEYKLAYLHVINPAA
                     AQLQNGEPIPDRVERMLAMIRDKFRGVLMLAGGFDHDSAEQWLEAGKADVIAFGRLFL
                     ANPDLPERFRRRAKLNEPDPTTFYGGGERGYTDYQSLEQEEGVKPRSTIDTSWR"
     gene            1365..>1576
                     /locus_tag="VF516_00040"
     CDS             1365..>1576
                     /locus_tag="VF516_00040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106075.1"
                     /translation="MATNKGPGTQEKQAGGAQPQATQAQPQGTAAQQPTAPAQQGAPR
                     QATTAMAPREDRGLARGGLPMFGPFSL"
BASE COUNT          271 a          535 c          541 g          229 t
ORIGIN      
        1 cgaggcgacg caggtgtcga tgcagggcca gggctatgcg tggacgcccg gcatccacag
       61 tcgcgagcag gtcgaaggct ggcgcgtgat caccgatgcc gtccacgacg cgggcggtcg
      121 catgttcatc cagctgtggc acgtgggccg gatctcgcac cccgcgctgc aacctgatca
      181 catgctgccc gtcgcgccgt cgccgatcac accggccggc aaggcgttca tcgagaacga
      241 gctcggcgag ggcgaattgg tgccgttcgt gcgaccgcgc gggctcgagc tcgaggagct
      301 gccgtatgtc gcgcgccagt acgcgcgcgg tgcgcgcaac gcgcagctcg cgaatgcaga
      361 cggtgtcgag gtccatggcg cgaacggcta cctgctcgag caattcctcg agacgggcac
      421 gaaccggcgt ggcgacgcgt acggcggctc cgtcgcgcat cgcgcacgca tgctgctcga
      481 tgtcgtcgag acctgcatcg cggtgtgggg acccgagcgc gtcggcgtgc ggatctcgcc
      541 gctgtcgacg ttcaacgaca tccacgacga caatcccgag gaaacattcg cctacatcgc
      601 cgagcagctc tcggaataca agctcgccta cctgcacgtg atcaacccgg cggcggctca
      661 gctccagaac ggcgagccga ttcccgaccg cgttgagagg atgctcgcga tgatccgcga
      721 caagttccgc ggcgtgctga tgctcgccgg cggcttcgac cacgattcgg ccgagcagtg
      781 gctggaggcc ggcaaggccg atgtgatcgc tttcggccgg ctgttcctcg cgaatcccga
      841 tcttcccgag cgctttcgcc gccgcgccaa gctcaacgag cccgatccca ccacgttcta
      901 cggtggcggc gagcgcggct acaccgacta ccagagcctc gagcaggagg agggcgtcaa
      961 gccgaggtcg accatcgaca cgtcgtggcg gtgatctgac tcacgaggat ctcgtgcggt
     1021 gaccgatcgc cggtgcgcgc tcggcgaacc gctcatggcg tggcgcccgg cgagaacttg
     1081 tgcactcgac cgggcggctc gcccgagtca cggcgcggag accgtgctgc tgatcgacga
     1141 cgacgagaga tccgagcgat cgcggctcgc tcgctgcggc aggccgcgta caacgtcctc
     1201 gtgccgagcg ggcccgagga cgccctccgg ctcgctcggt tcacgcccga agcgctcgtc
     1261 gcgaatgtac gtaccgtcct cgatcgccgt ggctggccgc gtggtccagc gaatgcagtt
     1321 cctccaggta gctcatctct tcgagagctg gaggaaaaca catcatggcg actaacaaag
     1381 gaccagggac gcaggagaag caggccggcg gtgcacagcc gcaggcgaca caggcgcaac
     1441 cccagggcac ggcggcgcag cagccgacag cgccggctca gcagggggcg cccaggcagg
     1501 cgacgaccgc gatggcgccg cgcgaagatc gcgggctcgc acgcgggggc ctgccgatgt
     1561 tcggaccatt ctcgct
//
LOCUS       DASVOR010000003         2504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58465,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000003 DASVOR010000000
VERSION     DASVOR010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58465"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..964)
                     /locus_tag="VF516_00045"
     CDS             complement(194..964)
                     /locus_tag="VF516_00045"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEX8106076.1"
                     /translation="MTPDPATRWIVRPRPNPRAALRLVCFPYAGSGATVFRTWPDALP
                     PEVELVAIALPGRDARAREPLVDRLAPLVTCLADEVGPQLRAPFAIFGHSFGAMLGFG
                     FARELQRRKLGQPVHFFASGRRAPQLPERTPLRRLPDPDLLAALRHMGGIPDAVFDVP
                     ELIDYFLPILRADIAASETDTIGLDAPLSCPITAMGGVSDERVSVDDLDAWRAQTTGP
                     FEREMYPGGHFFLQTDRDRFLSSLSWRLARAIVAHAAG"
     gene            1104..>2504
                     /locus_tag="VF516_00050"
     CDS             1104..>2504
                     /locus_tag="VF516_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106077.1"
                     /translation="MRYGKIAAFAIWLASTGVAHADITVTKARIGCLDIQQDGNLTGM
                     VAAACNHRASCSFQAPTEDEYRRAGVHAATRTFCTQAMEIVYTCGSGDFHTVTVPGDA
                     WKHPPAVLSCQAPPAPTTPPPPDRITVSRARIGCLDIQQDGNLTGMVGTACNGRDSCS
                     FPAPTPDAYQRAGVHAATRTFCTQAMEITYQCGHNDFQTVLVPGDAWAHPPAQLVCNP
                     LPVAAGHEFPPGAAAIKVEKARIGCLDIQTGGNLTQLVQQVCDDRSHCSYKAPTPDAY
                     QRAGVHAATRPLCTQAMEITYRCGSNDDQVVTVPGDAWNQPPAQLRCDGSSIATNRQD
                     VTPPPDARCSAPALAAPDYIAAPATLLDWSANSSYSDPIAGFRPAAQATRAQYNAPAG
                     SGITGAPGSTLGANEGRVRRELRDVARRRDAGHALCLAAQRFTTDRPGSATEPSDAAW
                     GDAFADLAVTGKAAFAS"
BASE COUNT          392 a          903 c          890 g          319 t
ORIGIN      
        1 gtgaccgagg cgacgctggc gcagtatccg gcgagctact acgatcgccc cgaggcgcgg
       61 atctacgctc gggtcaatcc gggctggcgc gacaacggcc gcaacgtcgg attctggttt
      121 cgcttcacgc cggcgaacct gagcaagctg gtccgcacga tctggaccaa gcgctgacgc
      181 ccccccggga gattcaccct gccgcgtggg cgacgatcgc gcgcgccagt cgccaggaca
      241 gcgagctcag gaatcggtcc cggtcggtct gaagaaagaa gtggcccccg ggatacatct
      301 cgcgctcgaa cggcccggtg gtctgcgctc gccacgcgtc gaggtcgtcc acgctgaccc
      361 gctcgtcgct gaccccgccc atcgcggtga tcgggcagct caggggtgcg tcgagtccga
      421 tcgtgtcggt ctcgctcgcg gcaatgtccg cccgcaggat cggcaggaag tagtcgatca
      481 gctcggggac gtcgaacacc gcgtcgggaa tgccgcccat gtggcgaagc gccgccagga
      541 gatcggggtc gggcagccgg cggagcggcg tgcgctcggg aagctgcggc gctcgccgcc
      601 ccgatgcgaa gaagtgaacc ggctggccga gcttccggcg ctgcagctca cgcgcgaagc
      661 cgaacccgag catcgcgccg aagctgtgcc cgaagatcgc gaatggggcg cgcagctgcg
      721 ggccgacctc gtcggcgagg caggtgacca gcggcgcgag ccggtcgacc agcggctccc
      781 gggcgcgcgc gtcgcggccg ggcagggcga tcgccacgag ctcgacctcg ggcggcaagg
      841 cgtcgggcca ggtgcgaaag accgttgcac cgctaccggc ataggggaag cacaccaggc
      901 gcagcgcggc ccgcgggttg gggcgcggac ggacgatcca tcgagtcgcg ggatccggtg
      961 tcatcgccac ggcgcccctt ggcgtctgcc catcatcgca ccacgatagc agctccggcg
     1021 accgggttct tcgacggccc gcggatcctg gggaagattt tcgaggagct gcgtcatggt
     1081 ggggtaagca agcgaggtgg agcatgaggt acggcaagat cgcggcgttc gccatctggc
     1141 tggcgagcac gggcgtcgcg cacgcggaca tcacggtcac caaggcgcgg atcggctgcc
     1201 tggacatcca gcaggacggc aacctgacgg gcatggtggc cgcggcctgc aaccatcggg
     1261 cgtcgtgctc gttccaggcg ccgaccgagg acgagtaccg ccgcgccggc gtccacgccg
     1321 ccacccgcac gttctgcacc caggcgatgg agatcgtcta tacctgcggg tccggtgact
     1381 tccataccgt caccgtcccg ggcgatgcat ggaagcaccc gcccgccgtg ctgtcgtgcc
     1441 aggcgccgcc ggcgccgacc acaccgccgc cgcccgaccg gatcacggta tcgcgcgccc
     1501 ggatcggctg cctggacatc cagcaggacg gcaacctgac cggcatggtc ggcacggcgt
     1561 gcaacgggcg agacagctgc tcgttcccgg cgccgacgcc ggacgcgtac cagcgcgcgg
     1621 gcgtccacgc cgcgacacgg acgttctgca cccaggcgat ggagatcacc tatcagtgcg
     1681 gtcacaacga cttccagacc gtgctcgtgc ccggtgacgc gtgggcgcat ccgccggcac
     1741 agctggtgtg caatccgctg ccggtcgccg cgggccacga gttcccgccg ggcgccgccg
     1801 cgatcaaggt cgagaaggcg cggatcggct gtctcgatat ccagaccggc ggcaacctga
     1861 cgcagctggt ccagcaggtc tgcgatgatc gcagccactg ctcgtacaag gccccgacgc
     1921 ccgacgccta ccagcgcgcg ggcgtccacg ccgcgacgcg gccgctgtgc acccaggcca
     1981 tggagatcac gtatcgctgc ggcagcaatg acgatcaggt cgtcaccgtc cccggcgatg
     2041 catggaacca gccgcccgcg cagctgcgct gcgacggcag ctcgatcgcg accaatcgcc
     2101 aggacgtgac gccgccgccc gacgctcgct gcagcgcgcc ggcgctcgcc gcgcccgact
     2161 acatcgcggc gccggcgacc ctgctcgact ggtcggcgaa ctcgtcgtac tcggatccga
     2221 tcgccgggtt ccgaccggcc gcgcaggcga cgcgcgcgca gtacaacgcg ccggcgggaa
     2281 gcgggatcac cggcgctccg ggcagcacgc tgggcgcgaa cgagggccgg gtccgccgcg
     2341 agctgcgcga cgtcgcccgg cgcagggacg ccggccacgc gctgtgcctc gccgcgcagc
     2401 ggttcacgac agatcgcccg ggctccgcga ccgagccgtc cgacgccgct tggggcgacg
     2461 cgttcgccga cctcgcggtc accggcaagg ccgcgttcgc gagc
//
LOCUS       DASVOR010000004         1724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_292316,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000004 DASVOR010000000
VERSION     DASVOR010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1724
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292316"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VF516_00055"
     CDS             complement(<1..558)
                     /locus_tag="VF516_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106078.1"
                     /translation="MRALLVFAALVAACNGSAPTEPKPGHGSAGSSAGAAASTAVPAS
                     KAGSLVEGATVVRPHREESVIECPAVVPESGDDEARVSALLDEANKQIERAQYAAAWT
                     CADRAGDLAPSSVEAQHLRGAALAALGRDQEAQLAYQLALALDPDDPETLRAVADFYI
                     NGKGERGRDALRLGLELAQRGSRKAE"
     gene            686..970
                     /locus_tag="VF516_00060"
     CDS             686..970
                     /locus_tag="VF516_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoD-like phosphatase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8106079.1"
                     /translation="MLTRRRFVIGALSTGAWACSRSAVVRSRPPRITHGVQSGDVQTG
                     RALIWARCDEPARMQVEWDTTMVDVTADALTVALRGIDGRERDRVELPHA"
     gene            1022..>1724
                     /locus_tag="VF516_00065"
     CDS             1022..>1724
                     /locus_tag="VF516_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106080.1"
                     /translation="MGSDSNVWHQVHAQREDGVAAMFLIRDVEPRADQPKIFVIELPY
                     PVTDLSGQPDAGAYRRLSAFQEQWIEPACAALGWTFVAWKCEDGSFFLYLYGAGDPDA
                     LLEKLAPFDGELGFFNDLDADWSEYAALRELVEQAEAEGEDEGEGEGEGEGEGEDDGD
                     GEHVHVHSDDCDHDPHDHDPPHHDHSHDHSHDHSHGALAPGIPVVEPAGDAAPPELSR
                     RNGKSRSIAAGSEAAA"
BASE COUNT          245 a          635 c          632 g          212 t
ORIGIN      
        1 ctcggccttg cggctgccgc gctgcgcgag ctccaggccg agccggagcg cgtcgcggcc
       61 gcgctcgccc ttgccattga tatagaagtc cgccaccgcg cgcagggtct cggggtcatc
      121 gggatcgagc gccagcgcga gctggtaggc gagctgtgct tcctggtcgc ggccgagcgc
      181 cgccagcgca gcgccccgca ggtgctgggc ctcgaccgag ctcggcgcca ggtcgcccgc
      241 gcgatcggcg cacgtccacg ccgcggcgta ctgcgcgcgc tcgatctgct tgttggcctc
      301 gtcgagcagc gccgacaccc gggcctcgtc gtcgccgctc tccgggacca ccgccgggca
      361 ctcgatcacc gactcctcgc ggtgcggccg caccaccgtg gcgccctcga ccagcgagcc
      421 ggccttggac gccgggaccg cggtcgacgc cgcggcgccg gcggacgagc cggcggaccc
      481 gtgccctggt ttcggctccg tgggcgccga gccgttgcac gccgcgacca gggcggcgaa
      541 aacgaggagg gcgcgcatcg agccaagaat accggacgcg gggggcgaac gcgaaacgct
      601 gacacaactg cgcgaacggc gaccgcggag cgaggggggc gccatggggc acagagacga
      661 cagcgattgc ggtacgttgt cggccgtgct gacccgccgg cgcttcgtga tcggcgccct
      721 gtcgacgggc gcctgggcct gcagccgctc ggccgtcgtg cgctcccggc caccgcggat
      781 cacccatggt gtgcagagcg gcgatgtcca gaccggccgg gcgctgatct gggcgcgctg
      841 tgacgaaccc gcccgcatgc aggtcgagtg ggacaccacc atggtcgacg tcaccgcgga
      901 cgcgctcacg gtggcgctgc gcggcatcga cggccgcgag cgcgaccgcg tcgagctgcc
      961 gcacgcgtga tcccggcgcc cgccggccgc gcaaccccgc gcgccgaggt aggctaccgc
     1021 gatgggttcc gattcgaacg tgtggcatca ggtacacgcg cagcgcgagg atggcgtcgc
     1081 ggccatgttc ttgatccgcg atgtcgagcc gcgcgccgat cagccgaaga tcttcgtgat
     1141 cgagctgccc taccccgtca ccgacctgtc gggccagccc gacgccgggg cctaccgccg
     1201 gctcagcgcg ttccaggagc agtggatcga gcccgcgtgc gcggcgctcg gctggacgtt
     1261 cgtggcctgg aagtgcgagg acggctcgtt cttcctctac ctctacggcg caggcgaccc
     1321 cgacgccctg ctcgagaagc tcgcgccgtt cgacggcgag ctcgggttct tcaacgacct
     1381 cgacgccgac tggtcggagt acgcggcgct gcgcgagctg gtcgagcagg ccgaggcgga
     1441 gggcgaggac gagggcgagg gcgagggcga gggcgagggc gagggggagg acgacggcga
     1501 cggcgagcac gtccacgtcc acagcgacga ctgcgaccac gaccctcacg accacgaccc
     1561 tccccaccac gatcacagcc acgatcacag ccacgatcac agccatggcg ccctcgcccc
     1621 ggggatcccg gtggtcgagc ccgccggcga tgccgcgccg ccggagctgt cccgccgcaa
     1681 cggcaagtca cggtcgatcg cggccggctc cgaggccgcg gcgg
//
LOCUS       DASVOR010000005         4135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_175406,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000005 DASVOR010000000
VERSION     DASVOR010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175406"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..965
                     /locus_tag="VF516_00070"
     CDS             114..965
                     /locus_tag="VF516_00070"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8106081.1"
                     /translation="MIDEPAFVGRAKTGSDARLVAVGSGGTSAGFLAAAFAIGVGFVV
                     VPVMLYLAMAPDHARFIGTCEGLPHPDDPYGVMLRVERASHDGKPVPAPVPAPVPAIE
                     VMDPLCPACKAFEQRLAASGLAGRLDRKAVLFPLDNACNWMVTEATHPGACTVSEAVL
                     CAGDKAPDVIAWAFEHGEQVRDAARTDPAAAARLVKQRFPELASCVGSPEARSRLNKS
                     MRWIVANNIRVLTPQLFVDKVKLCDEDVDLGLEYTLSAMLDRHARGALAIAPKETPVK
                     PSAGKAP"
     gene            962..1645
                     /locus_tag="VF516_00075"
     CDS             962..1645
                     /locus_tag="VF516_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106082.1"
                     /translation="MIAARIAIGAALLAVPAGLTGAWIRSADTRLTELSKAPAELAPV
                     ASASDVGYCSPELKRILRRVLMSCGLVGGNAARGCQPVQARSVATMSDHDFNALFKPM
                     QDRGGIVEFDKDKSELDPADVGLVDQVFADQRGASWFFVVARASPEGSALHNRELSRQ
                     RAEAVMAHLRQQFKDPDLDKEVGMLWLGDEYAQLEAEFCAWKRSSTAACNTDQLNRSA
                     FVAWIDCQL"
     gene            1624..2217
                     /locus_tag="VF516_00080"
     CDS             1624..2217
                     /locus_tag="VF516_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8106083.1"
                     /translation="MDRLSTLIAAAALIGCNTKAEPAGEPPAGTRTDTAKVKPAQTAS
                     TDAFCDVHAGDASGDVFHWPELAPGGTAPAGGAGWQWVNVWATWCKPCIAEMPRITAQ
                     RDKLVAAGKRVALTFVSTDDSDAEVAEFRKAHPELPGALRIAGNNQRIAWLRSLHLSD
                     GAIPINLFVSPAGRLRCARAGEIREQDLAMVDKLLAE"
     gene            complement(2249..2725)
                     /locus_tag="VF516_00085"
     CDS             complement(2249..2725)
                     /locus_tag="VF516_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106084.1"
                     /translation="MYVGKKAADALAEKMHIRGAIDLRAFAHDLVFGSGGPQTQAIRD
                     SLSTVLGLEPGGMAVLLESMAHDEAAMRIRYYWGAVIAPSRPDEDEDEDEDGPPVDDD
                     EPRQPLGATVQPIAAAPTQPATPSPAPPSDGASSPTLPDPRTTNGSTPTTSHDKAA"
     gene            complement(2759..3679)
                     /locus_tag="VF516_00090"
     CDS             complement(2759..3679)
                     /locus_tag="VF516_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106085.1"
                     /translation="MLGFAALCFVIALAAPGSMFDLPELRWLPPSEWPGWILQLHWFL
                     GFLIIAPFAVVAILGRDLRVTRERGQRLWLVARTPSLAGIPPEHAKEDEELWNKRQTA
                     AMTSWEYEQDRSGWLQGRLVLQALIFGDLTTFALLCSALLAASGLHADRNTVTQLACS
                     IAAATGTAFLANLARIMLRVSGGDVTTRTFSWAIRSVILVIIADIGLYVLLDGEAPSL
                     TSNVPHALLLGVFVGATGDHAIQFLLDKATKVFNAGEAAAHKASPLLEIEGVTDAHVD
                     RLEEEGIVSIHDLAFVPTARLFFSTTYSLQ"
BASE COUNT          613 a         1476 c         1473 g          573 t
ORIGIN      
        1 ccccggtgcg gcgcggccga aaggatgagc cgcgcgacgc gacgctcgga ggggtgaatc
       61 gcccggcttt gccgggcgag cccccagagg ggggttatgg gaaagccccc cttgtgatcg
      121 acgagcccgc gttcgtgggc cgcgccaaga ccggctcgga cgcccggctg gtcgcggtcg
      181 gaagcggcgg cacgtcggcc ggcttcctgg ccgcggcgtt cgcgatcggc gtcggcttcg
      241 tcgtggtgcc ggtcatgctg tacctggcga tggcgccgga tcacgcgcgg ttcatcggca
      301 cctgcgaggg cctgccgcac cccgacgacc cgtacggcgt gatgctgcgg gtcgagcgtg
      361 caagccacga tggcaagccc gtgccggcgc ccgtgccggc gcccgtgccg gcgatcgagg
      421 tgatggatcc gctgtgcccg gcgtgcaagg cgttcgagca gcggctggcg gcgtcggggc
      481 tcgccggtcg gctcgatcgc aaggcggtgc tgttcccgct cgacaacgcg tgcaactgga
      541 tggtcaccga ggcgacccat cccggggcgt gcacggtgag cgaggcggtg ctgtgcgccg
      601 gcgacaaggc gcccgacgtc atcgcctggg cgttcgaaca cggcgagcag gtccgcgacg
      661 cggcgcgcac cgatccggcc gccgcggcgc ggctcgtcaa gcagcggttc cccgagctgg
      721 cgtcgtgcgt cggctcgccc gaggcgcgat cgcggctcaa caagtcgatg cgctggatcg
      781 tcgccaacaa catccgcgtg ctgaccccgc agctgttcgt cgacaaggtc aagctgtgcg
      841 acgaggacgt cgacctcggc ctggagtaca cgctgtcggc gatgctcgac cgccacgcgc
      901 gcggcgcgct ggccatcgcc ccgaaggaga cgccggtcaa gccgtctgcg gggaaggcgc
      961 cgtgatcgcc gcgcggatcg cgatcggcgc cgcgctgctc gcggtgccgg cggggctgac
     1021 cggcgcgtgg atccggtcgg cggacacccg gctgaccgag ctgtcgaagg cgccggccga
     1081 gctcgccccg gtcgcctcgg cgtcggacgt cggctactgc agccccgagc tcaagcggat
     1141 cctgcgccgc gtgctgatga gctgcggcct ggtcggcggc aacgcggcgc gcggctgcca
     1201 gccggtccag gccaggagcg tcgccacgat gtcggaccac gacttcaacg cgctgttcaa
     1261 gccgatgcag gaccgcggcg ggatcgtcga gttcgacaag gacaagtccg agctcgaccc
     1321 ggccgacgtc gggctggtcg accaggtgtt cgccgaccag cgcggcgcga gctggttctt
     1381 cgtggtcgcg cgggcgtcgc ccgagggcag cgccctgcac aaccgcgagc tgtcgcggca
     1441 gcgcgccgag gccgtgatgg cccacctgcg ccagcagttc aaggaccccg acctcgacaa
     1501 ggaggtcggc atgctgtggc tcggcgacga gtacgcccag ctcgaggccg agttctgcgc
     1561 ctggaagcgc agctcgaccg cggcgtgcaa caccgatcaa ctcaaccgga gcgcgttcgt
     1621 cgcatggatc gactgtcaac tctgatcgcc gccgccgcgc tgatcggctg caacaccaag
     1681 gccgagcccg ccggcgagcc accggcgggg acgcggaccg ataccgccaa ggtcaagccg
     1741 gcgcagaccg cgagcaccga cgcgttctgc gacgtccacg ccggcgacgc cagcggcgac
     1801 gtgttccact ggcccgagct ggcccccggt ggcaccgccc cggcgggcgg cgccggctgg
     1861 cagtgggtca acgtgtgggc gacgtggtgc aagccgtgca tcgccgagat gccgcggatc
     1921 accgcgcagc gcgacaagct ggtcgccgcc ggcaagcgcg tcgcgctgac cttcgtgtcg
     1981 accgacgaca gcgacgccga ggtcgccgag ttccgcaagg cccaccccga gctgccgggg
     2041 gcgctgcgga tcgccggcaa caaccagcgc atcgcgtggc tgcgcagcct gcacctcagc
     2101 gacggcgcga tcccgatcaa cctgttcgtc agcccggccg ggcggctgcg ctgcgcgcgc
     2161 gccggcgaga tccgcgagca ggacctcgcg atggtcgaca agctgctcgc cgaatgacgc
     2221 gacctacgag cccggactca gcccggattc atgcagcctt gtcgtgtgag gtcgtcggcg
     2281 tgctgccgtt tgtggtccgc gggtcaggga gggtggggct cgaggcaccg tcggacggtg
     2341 gggccggtga cggcgtggcc ggctgcgtgg gtgcggcagc gatgggctgc acggtggcgc
     2401 ccagcggctg gcgcggttcg tcgtcgtcca ccggtggccc gtcctcgtcc tcgtcctcgt
     2461 cctcgtccgg tcgactcggc gcgatcaccg caccccagta gtagcggatg cgcatggcgg
     2521 cctcgtcatg tgccatcgat tcgagcagca cggccatccc ccccggctcg agcccgagga
     2581 cagtgctcag cgaatcccgg atcgcctggg tctggggccc gccggatccg aacaccaggt
     2641 cgtgcgcgaa cgcccggagg tcgatcgcgc cgcggatgtg catcttctcg gcaagtgcgt
     2701 cggcagcctt ctttccgacg tacacgtgca gcagcgcgcg atcctgccag ttgcagatct
     2761 actgcaggct gtacgtcgtg gagaagaaca gccgggcggt cggcacgaac gccagatcgt
     2821 gaatcgagac gatgccctct tcctcgaggc gatcgacgtg cgcgtcggtc acgccctcga
     2881 tctcgagcag cggggatgcc ttgtgggccg cggcctcgcc ggcgttgaac accttcgtcg
     2941 ccttgtcgag caggaactgg atcgcgtgat cgccggtggc tccgacgaac acgcccagca
     3001 gcagggcgtg cgggacgttc gacgtgaggc tcggcgcctc cccgtcgagc agcacgtaga
     3061 gcccgatgtc ggcgatgatc accaggatca ccgagcggat cgcccacgag aacgtccggg
     3121 tcgtgacgtc gccgccggac acccgcagca tgatgcgcgc gaggttggcc aggaatgcgg
     3181 tcccggtcgc ggcggcgatc gagcaggcga gctgcgtcac cgtgttgcga tcggcgtgga
     3241 gaccgctcgc cgcaagcagc gcgctgcaca gcagcgcgaa cgtggtcagg tcgccgaaga
     3301 tcagcgcctg caggaccagg cggccctgca accacccgct ccggtcctgc tcgtactccc
     3361 acgacgtcat ggcggcggtt tgccgcttgt tccacagctc ctcgtcttcc ttggcatgct
     3421 ccggcgggat gccagccagc gatggcgtgc gggccaccag ccacaaccgc tggccgcgtt
     3481 cccgggtcac gcgcaggtcc cggccgagaa tggccacgac cgcgaatggc gcgatgatga
     3541 gaaaccccag gaaccagtgc agctgcagga tccagcccgg ccactcgctc gggggcagcc
     3601 agcggagctc cgggaggtcg aacatcgaac ccggcgcggc gagtgcgatg acgaagcaca
     3661 gcgccgcgaa ccccagcacg aaggtgagcc ctgcacgggg agcattgcgc cgaccatcgc
     3721 tttgcgctga tcgctcgctc atcgcccgcc cccgcgcaac ccgcgccccg cgctgcacgc
     3781 tcgcggatcc gagcgatgac catccaccgg ctaccccatt ccctcgtctg tcgaacatgt
     3841 gccctcccgt gaaccacccg cgcgcatccg gctcggctca ccccgggccg agcttgcgca
     3901 cctcgaccac catatcagcg gtgaggcgaa tccgcgctgc cgcaattcac ggcagatcgc
     3961 cgtttgcacg gtccacccgg gggcggtact gcgaagatcc ccgccgcccc ggagcatgct
     4021 ctgccagcag ccgcagatat tccttcgaga tcagcgggcg ccgcgcagcg cggcggcgag
     4081 cgcgcggctg aggtcgccgc gccggccgac cacgacgcgg cccaggccga gccag
//
LOCUS       DASVOR010000006         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_263075,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000006 DASVOR010000000
VERSION     DASVOR010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263075"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF516_00095"
     CDS             <1..207
                     /locus_tag="VF516_00095"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX8106086.1"
                     /translation="KQLESEWLLALEGALSVVFGVLLLLSPLVGAIVLGLWIGAWALV
                     LGGMQIGTALRLRSYIHHHPELAA"
     gene            497..1369
                     /locus_tag="VF516_00100"
     CDS             497..1369
                     /locus_tag="VF516_00100"
                     /inference="COORDINATES: protein motif:HMM:NF025719.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8106087.1"
                     /translation="MATTTDRLTQVLQSSQVQLLADWIKALRAITKTRASAMIGDPEL
                     QAQCQEFIALMSAAVASGNLEDINAEPWRPVKDFLAGVSRSRGRQGFSPSDTATFVMS
                     FKEPLFANLRRTFEGMADSQVLTDVWNASLLLDKLGLFTTEVHQAAREELIRRQGEEM
                     LELSTPVIQLWDGILALPLIGTLDSARTQVVMESLLQQIVATRSPIAILDITGVPTVD
                     TLTAQHLLKTVAATRLMGAECIISGIRPQIAQTIVHLGVSLGDIITKASLADAFRLAL
                     SKLGQAITTAVAKG"
     gene            1373..1732
                     /locus_tag="VF516_00105"
     CDS             1373..1732
                     /locus_tag="VF516_00105"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8106088.1"
                     /translation="MDRIPILRMGRTLLVTVQVDMHDQLAVRLLDDLTTMITKTRARG
                     VVIDISALDMVDSFIGRMIADIAATSRILDAETVVVGMQPAVAITLVELGMALPGVRT
                     ALDVERGMELLDDARDR"
     gene            1713..2135
                     /locus_tag="VF516_00110"
     CDS             1713..2135
                     /locus_tag="VF516_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010883997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEX8106089.1"
                     /translation="MTPAIADPARPVIRVDIVENEDVVVVRQRAREQALRAGFSLLET
                     TKLVTAASELARNTLEHGGGGYAEIEVCTDGLRRGVRMRFIDEGPGIPDLEQALRDGF
                     TTGKGLGLGLGGSRRLVSEFEIQTAPGRGTKVTAIRWK"
BASE COUNT          334 a          776 c          803 g          337 t
ORIGIN      
        1 aagcagctcg agagcgagtg gctgctcgcg ctcgagggcg cgctgtcggt cgtgttcggg
       61 gtgctgctgc tgctgtcgcc gctggtcggc gcgatcgtgc tcggcctgtg gatcggcgcg
      121 tgggcgctgg tgctcggcgg gatgcagatc ggcaccgcgc tgcggctgcg gtcgtacatc
      181 catcaccacc ccgagctcgc cgcctgaccc acgccccggc gatcagcccc gctcgtacgg
      241 tgatcggcgc cggcgcccct tgcgcacggc gagatccgtg atcaccgatg aaggccgacg
      301 gtttttccga taagggctga cggctttccc gataggggtc gtgcggcgtc gcacatcgcc
      361 gggaccgggc ctccgaagtc agcgcagtgc ggcgctgtca tcggcatgac gcgcaccaga
      421 gtgtgccgcc gcgacctgga agatctgctt ccgggggcct tccgaccccc gtgagtgatt
      481 gctagcgtgg ctcgcgatgg ccaccaccac tgaccggctc acccaggtcc tgcaaagctc
      541 gcaggttcag ctgctcgccg actggatcaa ggcgctccgc gcgatcacga agacgcgagc
      601 gtcggccatg atcggcgatc ccgagctcca ggcgcagtgc caggagttca tcgcgctgat
      661 gtcggcggcg gtcgccagcg gcaacctgga ggacatcaac gccgagccgt ggcgaccggt
      721 caaggacttc ctcgccgggg tatcgcgctc gcgcggccgc caggggttct cgccgagcga
      781 caccgcgacg ttcgtgatgt cgttcaagga gccgctgttc gcgaacctgc gccgcacgtt
      841 cgaggggatg gcggacagcc aggtgctgac cgacgtgtgg aacgcgtccc tgctgctcga
      901 caagctcggg ctgttcacga ccgaggtcca ccaggcggcg cgcgaggaac tgatccggcg
      961 ccagggcgag gagatgctgg agctgtcgac gccggtgatc cagctgtggg acgggatcct
     1021 cgcattgccg ctgatcggca cgctcgactc ggcgcgcacc caggtcgtga tggagtcgct
     1081 gctccagcag atcgtcgcga cccggtcgcc gatcgcgatc ctggacatca ccggggtgcc
     1141 gacggtggac accctgaccg cgcagcacct gctgaagacg gtcgccgcga cccggctgat
     1201 gggcgcggag tgcatcatca gcgggatccg gccgcagatc gcccagacca tcgtccacct
     1261 cggggtctcg ctcggggaca tcatcaccaa ggcatcgctc gccgacgcgt tccgcctcgc
     1321 gctcagcaag ctcggccagg cgatcaccac ggcggtcgcg aaggggtgaa ccatggaccg
     1381 gatcccgatc ctgcggatgg gccgcacgct gctggtcacc gtgcaggtcg acatgcacga
     1441 ccagctcgcg gtgcgcctgc tcgacgacct gaccacgatg atcaccaaga cccgcgcgcg
     1501 cggcgtggtg atcgacatct cggcgctcga catggtcgac tcgttcatcg gccggatgat
     1561 cgcggacatc gcggcgacct cgcggatcct cgacgccgag accgtggtgg tcggcatgca
     1621 gcccgcggtc gcgatcacgc tggtcgagct cggcatggcg ctgcccgggg tacgcaccgc
     1681 gctcgacgtc gagcgcggca tggagctgct cgatgacgcc cgcgatcgct gatccagcac
     1741 gcccggtgat ccgcgtcgac atcgtcgaga acgaggatgt cgtggtcgtg cgccagcgcg
     1801 cgcgcgagca ggccctgcgc gccgggttct cgctgctcga gaccaccaag ctggtcaccg
     1861 ccgcgagcga gctcgcgcgc aacacgctcg agcacggcgg cggcggctat gccgagatcg
     1921 aggtctgcac cgacgggctg cgccgcgggg tccggatgcg attcatcgac gaggggcccg
     1981 ggatccccga cctcgagcag gcgctgcgcg acgggttcac caccggcaag gggctcggcc
     2041 tggggctcgg cggctcgcgc cggctggtca gcgagttcga gatccagacc gcgcccggcc
     2101 gcggcacgaa ggtcacggcg atccgatgga agtgaccgcg ctgtccgagc cgttcgaact
     2161 cgaggccagc gccgacgtcg gcgtggtgcg ccggcggctc gccgcgctgg ccgccgaggt
     2221 cgggctcgac gaggtgcgcg ccggtgacgc
//
LOCUS       DASVOR010000007         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_175410,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000007 DASVOR010000000
VERSION     DASVOR010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175410"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..855
                     /locus_tag="VF516_00115"
     CDS             94..855
                     /locus_tag="VF516_00115"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX8106090.1"
                     /translation="MRLAALVLAGLATSATSAASAAADTSKLGKEHLAVQPKAVIRGK
                     NKLCCGYPMVDDQQWALRFYWLTRETDYDDAPPDSIPRRGACALPANRWVELYTHDGY
                     FFGRVPERYACSLKLEGSGLMRDDRIVNYTGACKFGYGTCFEQLDVGDFPFGRGAGLR
                     PLIPFKSVAVDPRVVPLGEPLYIPEFDGMVLPDGSIHDGCVRADDTGGGIKGRKLDFF
                     VVTYGNFRTLLDELLGVTWITPEVEAPRCDYLRDR"
     gene            876..1376
                     /locus_tag="VF516_00120"
     CDS             876..1376
                     /locus_tag="VF516_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106091.1"
                     /translation="MLGLFVTPTVVAIGDALTSIASAGAPGAIAVVGNARLAAALGAT
                     REVIPVGLSPRSAKKLRGTARELSAIEPASLAALVGVDLAVEIAWEEALRSWSRVVRD
                     GGAIVFVDRGHAHEASRRALCGGLTEIEQRRAGRAVITSGVVSHFGPEELAVPPPSPP
                     ASSEPA"
     gene            complement(1377..>2939)
                     /locus_tag="VF516_00125"
     CDS             complement(1377..>2939)
                     /locus_tag="VF516_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX8106092.1"
                     /translation="ASAYENADFGARHQDKREGWRGPEWRLDGAARALFLGRQRALYG
                     NAPLAPGKRYLALVDKVDKDHAEVLIGDRRLQLPLRNLKWAAKWQAGNAENDAEIDKA
                     TDALKPGYVVWVGRELRTLPRYRDWTLEMKNPTWIARDDQAEWDAAHTDIVKLDQVPH
                     PQTAIFTADHHTGYVAAMVGGSDYDRSEFNRTVQACRQPGSTYKPIYYSLGLDQGYGF
                     DTVLNDVKVDIVDPVTGEVWSPNNLEGTSDGDVTLEFALVFSKNIPSVDLFRRLGAAN
                     VEAWARRLGITTKIFPDDALALGASCSRLDEMARAFTVFARLGAWWPRPGGREKNWVY
                     VRRILDRAGNAVEDNTVAEDPALAPPDRFDRAAVLAGIAAPQAISPRTAFLMTKLLAH
                     EVEYGFANVLRATQVNAAGKTGTSSATFDNLFIAFTSKFTTLVWMGDDKRQRALGKSD
                     AAYMTVVPLWARYMYEAARSYPNPPIPWVIPPDVKPDDRGDHSKGVHGPQMDLIWKAP
                     AKKTGDDTRPPV"
BASE COUNT          379 a         1028 c         1075 g          457 t
ORIGIN      
        1 ccttcgcgcc gcacgaggcg tcggcgtcgc ggagcgagcg gatcgccatg gcggcgttgt
       61 accgcgcgcg gccggagctg ataagcttgc cgggtgcgcc tcgcagcgct tgtgctggcc
      121 ggcctcgcaa cctcggcaac ctcggcagcc tcggcggccg ccgacaccag caagctcggc
      181 aaggagcacc tggcggtcca gcccaaggcg gtgatccgcg gcaagaacaa gctgtgctgc
      241 ggctacccga tggtcgacga tcagcagtgg gcgctgcggt tctactggct gacccgcgag
      301 accgactacg acgacgcgcc gcccgacagc atcccgcgcc gcggggcgtg cgcgctgccg
      361 gccaatcgct gggtcgagct ctacacccat gacggctact tcttcggccg cgtccccgag
      421 cgctacgcgt gctcgctcaa gctcgagggc tcgggcctga tgcgcgacga tcggatcgtc
      481 aactacaccg gcgcgtgcaa gttcggctac ggcacgtgct tcgagcagct cgacgtcggc
      541 gacttcccgt tcggccgcgg cgccggcctg cgcccgctga tcccgttcaa gtcggtcgcg
      601 gtcgatccgc gcgtggtccc gctcggcgag ccgctctaca tccccgagtt cgacggcatg
      661 gtgctgcccg acggctcgat ccacgacggc tgcgtgcgcg ccgacgacac cggcggcggg
      721 atcaagggcc gcaagctcga cttcttcgtc gtcacctacg gaaacttccg aacgctgctc
      781 gacgagctgc tcggcgtcac ctggatcacg cccgaggtcg aggcgccgcg ctgcgactac
      841 ttgcgcgacc gctagcaacg gcagtagcgt ggcgcgtgct cggtctgttc gtcacgccca
      901 cggtcgtcgc gatcggggat gcgctcacat cgatcgcgag cgccggggcg ccgggggcga
      961 tcgcggtggt cggcaatgcc cggctcgccg cggcgctcgg cgcgacccgc gaggtcatcc
     1021 cggtcggcct gtcgccccgg tcggccaaga agctgcgcgg caccgcgcgc gagctgtccg
     1081 cgatcgagcc ggcgtcgctc gccgcgctgg tcggcgtcga cctcgcggtc gagatcgcct
     1141 gggaggaggc gctgcgcagc tggtcgcggg tggtccgcga cggcggcgcg atcgtgttcg
     1201 tcgaccgcgg ccacgcccac gaggcgagcc gccgcgcgct gtgcggcggg ctgaccgaga
     1261 tcgagcagcg ccgcgcgggc cgtgccgtga tcaccagcgg cgtggtcagc cacttcggcc
     1321 ccgaggagct ggccgttccg ccgccaagtc cgccggcgag cagtgaacca gcctgactat
     1381 accggcgggc gggtatcgtc gccggtcttc ttcgccggcg ccttccagat caggtccatc
     1441 tgcgggccgt gcacgccctt ggagtggtcg ccgcggtcgt cgggcttgac gtccggcggg
     1501 atgacccacg ggatcggcgg gttggggtac gaccgcgcgg cctcgtacat gtagcgcgcc
     1561 cacagcggca cgaccgtcat gtacgcggcg tcgctcttgc cgagcgcgcg ctgccgcttg
     1621 tcgtcgccca tccacaccag cgtggtgaac ttggaggtga acgcgatgaa caggttgtcg
     1681 aacgtcgcgc tcgacgtccc ggtcttgccg gcggcgttga cctgggtcgc gcgcaggacg
     1741 ttggcgaacc cgtactcgac ctcgtgggcg agcagcttgg tcatgaggaa cgcggtgcgc
     1801 ggcgagatcg cctggggcgc cgcgatgccg gcgagcacgg cggcgcggtc gaagcggtcg
     1861 gggggcgcca gcgcggggtc ctcggcgacg gtgttgtcct cgaccgcgtt gccggcgcga
     1921 tcgaggatcc ggcggacgta gacccagttc ttctcgcggc cgcccgggcg cggccaccac
     1981 gcgccgagcc gcgcgaacac ggtgaacgcg cgcgccatct cgtcgagccg cgagcacgac
     2041 gcgccgagcg cgagcgcgtc gtccgggaag atcttggtgg tgatcccgag ccgccgcgcc
     2101 caggcctcga cgttggccgc gccgagccgg cggaacaggt cgaccgacgg gatgttcttg
     2161 gagaacacca gcgcgaactc cagcgtgacg tcgccgtcgc tggtgccctc gaggttgttg
     2221 ggcgaccaca cctcgccggt caccgggtcg acgatgtcga ccttgacgtc gttgagcacg
     2281 gtgtcgaacc cgtagccctg gtcgaggccg agcgagtagt agatcggctt gtaggtcgag
     2341 cccggctggc ggcaggcctg gaccgtgcgg ttgaactcgg agcggtcgta gtccgagccg
     2401 ccgaccatcg cggccacgta gccggtgtgg tgatcggcgg tgaagatcgc ggtctgcggg
     2461 tgggggacct gatcgagctt gacgatgtcg gtgtgcgccg cgtcccactc ggcctggtcg
     2521 tccctggcga tccaggtcgg gttcttcatc tccagcgtcc agtcgcggta cctgggcagc
     2581 gtgcggagct cgcggccgac ccacaccacg tagccgggct tgagcgcgtc ggtcgccttg
     2641 tcgatctcgg cgtcgttctc ggcgttgccg gcctgccact tcgccgccca cttcaggttg
     2701 cgcagcggca gctggagccg gcgatcgccg atcagcacct cggcgtgatc cttgtcgacc
     2761 ttgtcgacca gcgcgaggta gcgcttgccg ggcgcgagcg gcgcgttgcc gtacagcgcg
     2821 cgctggcggc cgaggaacag cgcgcgcgcg gcgccgtcga gccgccactc ggggccgcgc
     2881 cagccctcgc gcttgtcctg gtggcgcgcg ccgaagtcgg cgttctcgta ggcgctggc
//
LOCUS       DASVOR010000008         3161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58488,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000008 DASVOR010000000
VERSION     DASVOR010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58488"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..>3161)
                     /locus_tag="VF516_00130"
     CDS             complement(163..>3161)
                     /locus_tag="VF516_00130"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8106093.1"
                     /translation="PGASPAAVETDVTQKVEEAVNTVSGLDTLTSSSTEGVSLVIAQF
                     DLEVDPDKASNDINEHLATILRDLPPGTRPEVRKADPDAAPVIVLSVKGPAGVPIREL
                     TRFADKQVKQRIERLSGVGQVVILGGQDRQINVHLDAIRLAGAGVSAIDVHRAIANGN
                     VNVPGGTIERGPLNTTLRVESRALQADQIGDIVVRQLGDHPVKVRDVAQVVDSEKDAE
                     TSAAYDGEPAIALSIRKQTGTNTVAVVDSVQKAVNKLKTQLPQGYTVDVVRDNSVQIR
                     TSASQVLEHLVLGALLAALIVLLFLGSLRSTVIAAISIPVSIVSTFGLMRAAGFTLNL
                     MTLLALALAVGIVIDDAIVVLENIYRFIDEKRMKPFPAAIHATKEIGLAVLATTLSLM
                     AVFLPVAFMSGIIGKFLYSFGMTMAFAIAVSMLVAFVLTPMMAARMLPLPAPPGRERR
                     KTWLEHGSNLLYRPIERLYSGVLAFCLRHRWVVLIAIISSCVTTFPMGKKVGFDFLPP
                     NDEAQFEIYLQTPENATLETTTLVAERLARKARELPEVEHTLVSVADSDQRQANVGHI
                     YIHLTPPETRVRSQLLVMAAVREKVLVPTNYPAGTVVAVQAVNDFSIGGQNANISYVI
                     SGPDLDKLQYYSDTIMARMKAVPGVVDLTSSLLDPVDETTVVPDLDRAGLLGVEPADI
                     TATLAILVGGVDASTFEDRGDQYQVFLRAAERYRNDPAALSLIAVPSRSLGQVPLSDV
                     IKVRASQAISRITRQSRERAVTITMNNSPGFSESAIVAELQKTINALHLPPGYTADPF
                     GRSKESGKTLMAFVIALGLAFLFMYLVLAAQFESWLYPVIIMMSLFLVIPFAVMSLVI
                     TGGSLNIFSMLGIFVLFAMVKKNAILQVDHANGLRRQGLPRTEAVLAASRDRLRPILM
                     TTFAFVAGMLPLVTSKGVGAGFSKAMASVVVGGQTLSLLLTLVAIPVLYTLFDDLARW
                     VSRLLFGDKPPPDRGKAEVGVVDIHA"
BASE COUNT          519 a         1059 c         1092 g          491 t
ORIGIN      
        1 gagggattcg cgtcatacca aagctgcccc aggatcggaa cccgaattcg tcgcaaagtc
       61 acgcgggtgc cacgctccgg agcgcaacgg cggtagggcg catcaccgtt ttatcctgtg
      121 aaatcccttc ggccaagccc cagtgcacgc ccgtcgcggc ccctatgcgt ggatgtccac
      181 cacgccgacc tcggccttgc cgcgatccgg gggtggcttg tctccgaaca gcaggcggga
      241 cacccagcgg gccaggtcat cgaacaaggt gtacaggacc gggatcgcga ccagcgtcag
      301 caggagcgac agcgtctggc cgccgaccac gacgctcgcc atcgccttgg agaaccccgc
      361 tccgacgccc ttgctggtca ccagcggcag catgccggcg acgaacgcga acgtggtcat
      421 caggatcggc cggagccggt cgcggctcgc cgcgagcacc gcctcggtgc gcggcaagcc
      481 ttgccgccgc agcccgttgg cgtgatcgac ctggaggatc gcattcttct tgaccatcgc
      541 gaacagcacg aagatgccga gcatcgagaa gatgttcagg ctgccgccgg tgatgaccag
      601 cgacatcacc gcgaacggga tgaccaggaa cagcgacatc atgatgatca ccgggtacag
      661 ccaggactcg aactgcgcgg cgagcaccag gtacatgaac aggaacgcca ggccgagcgc
      721 gatcacgaac gccatgaggg tcttgccgga ctccttgctg cggccgaacg ggtcggcggt
      781 gtaacccggc ggcaagtgga gcgcattgat cgtcttctgc agctcggcga cgatggccga
      841 ctccgagaac cccggcgagt tgttcatcgt gatcgtcacc gcacgctcgc ggctctgccg
      901 cgtgatcctc gagatcgcct ggctggcgcg gaccttgatc acatcggaca gcgggacctg
      961 accgagcgag cgcgacggca ccgcgatcag cgacagcgcc gccgggtcgt tgcggtagcg
     1021 ctcggcggcg cgcaggaaca cctggtactg atcgccgcgg tcctcgaacg tcgaggcgtc
     1081 gaccccgccg accaggatcg ccagcgtcgc ggtgatgtcg gcgggctcga ccccgagcag
     1141 gccggcgcga tcgaggtcgg gaaccacggt ggtctcatcg accggatcga gcaagctgga
     1201 ggtgaggtcg acgacgccgg gcacggcctt catccgggcc atgatcgtgt cgctgtagta
     1261 ctggagcttg tcgagatccg gcccgctgat cacgtaggag atgttggcgt tctggccgcc
     1321 gatcgagaag tcgttgaccg cctggaccgc caccaccgtg cccgccgggt agttggtcgg
     1381 caccagcacc ttctcgcgca ccgcggccat cacgagcaac tggctacgca cgcgcgtctc
     1441 gggtggcgtg aggtggatgt agatgtggcc gacgttggcc tggcgctggt cgctgtcggc
     1501 caccgacacc agcgtgtgct ccacctccgg gagctcgcgg gccttgcgcg cgagccgctc
     1561 ggcgaccagc gtggtcgtct ccagcgtcgc gttctcgggg gtctggaggt agatctcgaa
     1621 ctgggcctcg tcgttgggcg gcaggaagtc aaagccgacc ttcttgccca tcgggaacgt
     1681 ggtgacgcag ctcgagatga tcgcgatcag cacgacccag cgatggcgca ggcagaacgc
     1741 cagcacgccc gagtacaggc gctcgatcgg ccggtacagg aggttcgagc cgtgctcgag
     1801 ccacgtcttg cggcgctccc ggccgggcgg cgccggcagc ggcagcatgc gcgccgccat
     1861 catcggcgtc agcacgaacg cgaccagcat cgacaccgcg atcgcgaacg ccatggtcat
     1921 gccgaagctg tacaggaact tgccgatgat gccgctcatg aacgcgaccg gcaggaacac
     1981 cgccatcagc gacagcgtgg tcgccagcac ggcgagcccg atctccttgg tcgcgtggat
     2041 cgcggccggg aacggcttca tccgcttctc gtcgatgaac cggtagatgt tctcgagcac
     2101 gacgatcgcg tcgtcgatca cgatgccgac cgcgagcgcg agcgcgagca gggtcatcag
     2161 gttgagcgtg aacccggccg cccgcatcag gccgaacgtc gagacgatcg acaccgggat
     2221 cgagatcgcg gcgatcacgg tcgaccgcag gctgccgagg aacaagagca cgatcagcgc
     2281 cgcgagcagc gcgccgagca ccaggtgctc gagcacctgc gaggccgagg tccggatctg
     2341 gaccgagttg tcgcgcacga cgtcgaccgt gtagccctgc ggtagctggg tcttgagctt
     2401 gttgaccgcc ttctgcacgc tgtcgaccac cgcgaccgtg ttggtgccgg tctgcttgcg
     2461 gatcgacagc gcgatcgccg gctcgccgtc gtacgccgcc gaggtctcgg cgtccttctc
     2521 ggagtcgacg acctgggcga cgtcgcggac cttgaccggg tgatcgccga gctggcggac
     2581 cacgatgtcc ccgatctggt cggcctgcag cgcgcggctc tcgacccgca gcgtcgtgtt
     2641 gagcgggccg cgctcgatcg tcccgcccgg gacgttgacg ttgccgttgg cgatcgcgcg
     2701 gtggacgtcg atcgccgaga ccccggcccc ggcgagccgg atcgcgtcga ggtggacgtt
     2761 gatctggcgg tcctggccgc cgaggatcac gacctggccg acgccggaca gccgctcgat
     2821 ccgctgcttg acctgcttgt cggcgaaccg ggtcagctcg cggatcggca cgccggccgg
     2881 gcccttgacc gacagcacga tcaccggcgc ggcgtcgggg tcggccttgc ggacctcggg
     2941 gcgggtgccc ggcggcaggt cgcgcaggat cgtggcgagg tgctcgttga tgtcgttgga
     3001 cgccttgtcg ggatcgacct cgaggtcgaa ctgcgcgatc accagcgaca cgccctcggt
     3061 cgagctcgag gtcagggtgt cgagcccgct gaccgtgttg accgcctcct cgaccttctg
     3121 ggtcacgtcg gtctcgaccg cggcgggcga cgcgccggga t
//
LOCUS       DASVOR010000009        21353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_233850,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000009 DASVOR010000000
VERSION     DASVOR010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21353
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233850"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..657
                     /locus_tag="VF516_00135"
     CDS             148..657
                     /locus_tag="VF516_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8106094.1"
                     /translation="MRLDLGDVQLDITAHVPGLRRYGIRLHVGAPRDTTAADRTPRGR
                     LGPTPRKVDFIMDLMADKQVDVDVQWTDEIGHPVDAPAGATVSWTTDNPTVVAVTQHE
                     DGTATVAATGELGTANVSVSVSHDGRTFTGDAQVNVVAGLAERVNIVFGAPTEVTPDE
                     PPAEPAPVL"
     gene            899..1969
                     /locus_tag="VF516_00140"
     CDS             899..1969
                     /locus_tag="VF516_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEX8106095.1"
                     /translation="MTRTGSRLVWDVRPDLDGFDHPHLLGVTPSGRPIWSASGGAPID
                     VSSWIPIEYDSDVVQRVRMESVIEGRARRVVMGSKTKSIPRSAGVTVTVGTTYTDDAS
                     TNDEVTLTARRFISRVQIDEDDLADASTRMDVLGTKSLDWAISYADTFDNACLGVTAT
                     ETGTATAPFTSAYKRLRSTAAGLGYTADDNYTTWDDDLISIAATPNGTSLYEKLSFIF
                     KKVETKAYWSQPDMLVIAHPGWRDALRLCTDAQGRPIFTSGGGAPGNGTPDTLFQTPI
                     AWSRGCKTSPVLQTSPTGNDLLYFVNVRYLARGDRSGPEVLTDDARAQDDNDNYAMKF
                     RARRGFQLTHPNAAAVLERITD"
     gene            2064..2417
                     /locus_tag="VF516_00145"
     CDS             2064..2417
                     /locus_tag="VF516_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106096.1"
                     /translation="MVVDVTAVELNIDLAQNRTFELVAAVLASDGVTPRDDLAGWTGA
                     MQIRDSRSTAATLLAEADVTVDATTGTVTARVPAADTDGATWRSGAYDLYITNGTARE
                     DLAYGVARLRRAVTA"
     gene            2428..2550
                     /locus_tag="VF516_00150"
     CDS             2428..2550
                     /locus_tag="VF516_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106097.1"
                     /translation="MAAKKGKKKTVHTKTYSAMRRRGVGKGAATKMAARAARKC"
     gene            2559..2972
                     /locus_tag="VF516_00155"
     CDS             2559..2972
                     /locus_tag="VF516_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106098.1"
                     /translation="MGKLLVVRKRADRTGPEPAPGQPWPLLGVELVDAPDTCRVPTNW
                     VNKAVAEGWAELVGPAVVMRPGGPASDPASVLHQFLHAEALRIHTLDGPVTYRITRQP
                     DKYVAGGADDEPVTAEAYAAGDTQVDHWYELEREV"
     gene            2974..3447
                     /locus_tag="VF516_00160"
     CDS             2974..3447
                     /locus_tag="VF516_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106099.1"
                     /translation="MADLVFNRSKGRVVQFAELINGNATPYVNAAFIIEAINTSATDA
                     TLRDLDDFAAIEADGNTAEVTNSGYARKTVTEAASAITITYDDTNDRVDVDLPDQTWT
                     AVGAGTAWTDLVVGFDNDTTGGTDSNILPCTLHDFAITPDGSDITATINVFYRAS"
     gene            3463..4704
                     /locus_tag="VF516_00165"
     CDS             3463..4704
                     /locus_tag="VF516_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106100.1"
                     /translation="MPATLIGSNTYAFADGNAGHVIDLGSSPSVGQTDVLCVNSDTVV
                     TTPSGFALDVSRVNSQGSYIYRRKAVGGEGQTVTITTSGNNNTSVSWLRWGGLNAKDA
                     TANSGVDGVAGTSSPAHSSGTMTASTELIIAFAALHSFGAVPSSPVWANSYTGQTAVT
                     QGSGSTGAGNFVGYKQPVGTAAESPSVSWTGNVSDRYMLTITFTITGGLSGTAGTATE
                     TDTAQPIGKRKTRAVGTTSTTDTAQPIGRRKTRAVGVASEADTAGTLGKRKTRALAVA
                     GGTDTAGTITRTKTRAVGTASDASATLTLGRLKARVVGTATTTDTARTLTAGHLRALG
                     TAAEVDTARTITTVHAIVRAVGTALEVDTAPPVTASGGLPVIRRPTIDVVTGPLHVAV
                     VDAPAVAVVAGGVRVVVVSDG"
     gene            4759..5382
                     /locus_tag="VF516_00170"
     CDS             4759..5382
                     /locus_tag="VF516_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106101.1"
                     /translation="MTAAVAEITATDTWINASTLSRPQLMKAAAARGLQQVGTNVELV
                     NRINQWQQEQGLYPPPPPDEDELDLEVGDDEDDDEPGPAPEAVGTERAPDPDEDEDPE
                     PAPLPMPAAPTMKLPAAGKVASSPAAGVKKRRTGEFPSGWRAEYRVGWSGINDELHFR
                     LIEETHHRAVEAGYRTKGAPYAGLRIGYHGQGDARTVVYEVAISRED"
     gene            5382..5888
                     /locus_tag="VF516_00175"
     CDS             5382..5888
                     /locus_tag="VF516_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106102.1"
                     /translation="MGTWATIETVFAVTRREVSEDDLALAQSIIEDFAGTDVDFTDDS
                     AIGTRNQRYLRKAVCWQAAWLSTHPDALDAMDVTGVSGDGVSAQHATDTAAFMAPMAR
                     RCLNRVTWRNGPIRIRPGRRAGVVDSGDRDDAVRDDAFVWSPLSSEGSPRIGHADPLS
                     ERWGQVWR"
     gene            5927..6058
                     /locus_tag="VF516_00180"
     CDS             5927..6058
                     /locus_tag="VF516_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106103.1"
                     /translation="MAYFVALPVAAFVSSWAGLALLAFFLVATFPSLFAGGLPRRRS"
     gene            6079..6387
                     /locus_tag="VF516_00185"
     CDS             6079..6387
                     /locus_tag="VF516_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106104.1"
                     /translation="MTVLRGTGTDRFGDEVDTDTVVYTQRPASIIEQQPNSRSRPAEG
                     RTDQVRGYALRMHSNTRLLKGDRIRDERTGDTYTVDFLATPANPAGHTSRRATLRKVT
                     "
     gene            6484..6873
                     /locus_tag="VF516_00190"
     CDS             6484..6873
                     /locus_tag="VF516_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106105.1"
                     /translation="MPVGSTYENIRVNHGYEAWFLAVQGKSVRKLTEDIADDARAGCP
                     IDSGDLVESIGTRYPGGLRGIVVVGTDHWVPTEYGSPPHIIYSHGPWSLRSDEGEYFG
                     RVVHHPGTPAQPFMRPALYQRRKPVTV"
     gene            6870..7349
                     /locus_tag="VF516_00195"
     CDS             6870..7349
                     /locus_tag="VF516_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106106.1"
                     /translation="MTTPLLPRLANNELVALAWIRDIVSVYNVALGTTLQGPDPDTQV
                     LSWGDTGFVTASTVGGTPNGTVPLREPVMEILCHATNPGKSRPPWGRANAIAETIIEA
                     ARSINWGDTQRPVTLPTGYPAVRVGDGSALTEPERRRSDEANYAIFGFTLSVSWLAL"
     gene            7377..7964
                     /locus_tag="VF516_00200"
     CDS             7377..7964
                     /locus_tag="VF516_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106107.1"
                     /translation="MANKLAYQVIMGAGSLYSGLYQATEPLDQAVNTSPQASAWVDAG
                     FTNDGVTITLNQEFATMTVDQISDIIGRKMTQRDVQVKANLAEATLENLTIGLNSGTI
                     STGSGGGVNYKVYTPVFNGSELQPTYFASIFDGYAPASAAGLTKRRRFILRRVLSIDN
                     VEVAYKKGDMTLVPVTFGCHYIDTVTAPFKIVDET"
     gene            8106..8471
                     /locus_tag="VF516_00205"
     CDS             8106..8471
                     /locus_tag="VF516_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106108.1"
                     /translation="MTTPYTPGKAGPIRINVKAKAKPEALERETFLHLDDPDTGESTE
                     VTIAKELLPSQGLRFITLVRDLGELRAVAQMFDEVIKPKGVLDRLAKIEGITQGEIDD
                     IMSVVTTKLMAAAEAAQGN"
     gene            8498..8842
                     /locus_tag="VF516_00210"
     CDS             8498..8842
                     /locus_tag="VF516_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106109.1"
                     /translation="MLEHLDDLESDFSAIHHIHIDFEAEQYGGLSSRRFFALAGRIMA
                     YPGVVQARYMAEEQRRQEDHEGVPITDTDRPRTAPRAEPGVKVIPLTPELLANGVDAL
                     GLSGVVEYATSG"
     gene            8859..11792
                     /locus_tag="VF516_00215"
     CDS             8859..11792
                     /locus_tag="VF516_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106110.1"
                     /translation="MALGYRIADGYMRVDADHSPADRSLAGFFRAADGRLRDMRGRFA
                     AEGQLAGQEYTKRFQEAAKNQKQSGFEGFARKLLGRFGALGKAAGGLLANKMVLAAVA
                     GLGALPTAIAAVGQAVGGLLNVAGAGAALAPAGIAAAAFSLITLKVALSGVGAAFKAG
                     LSGDAEKFAEALKKLAPAAQEAVKQLVALAPAARQLKQTVQNSLFAPWLDDIKPLASI
                     YLPILTQEMTWLAAAAGMAFHHVLELFETPAAAQQFHDMLTGFVQAFSNVAAGLPGIV
                     SGFFQLASAGSLFLGRFTEGFAGLTQRFAEWATAFTQSGRFDAFVEHALETLGGLSRA
                     LQDIVGIFQSFNRAAQAVVGGGLFQALGQILDMVNRFFQSVQGQEVLTALLTKLQTLG
                     GIVMDLVSGALPGLLSLSGALLEGLQALAPVAGPVGEALGAALSALAPILPVLGSLLA
                     PVLKLAAGALQSLATVLAPVIQLFSELATQALPLLMPLIDELVANALPMAAALATELY
                     NAFAPLVPVLLEVYHAIADGLMAALPDLLETNKELIPVVGEIAKIFADTWLSVLQELR
                     PHIPIIIQFFVALLKVSMAIGLTYLKIIPYVATFASWFIKAAAWVAGFVLTIIEIPTH
                     LRAIIGVLKEWVSGAVSAVGDFFGMIGGWISQAAQWFADLPGKIIGFLQQIPQAVRDA
                     LSAAFDAFFYWTGFIAAKVVEFVQSIPLRIAMMWQTIKTTFMDGVSAVGNFFAELPVK
                     AEFFFSDMYNKVTTWVVNTYNSVGQWLGSLPTRAGNALSSLWSSIRGAFDTATTNVRK
                     AIGELIDGAVTLAKSLPGKIRDAVGNLGHLLYDAGRDIVHGLIDGFNSMIDWAKNLVG
                     NAMKKIKDGAKAAIGSNSPSKAFRDEVGKTIPQGIAAGIRAGMPDLERYLTMRVAALP
                     GMSSANVNVAAPNVAVGAPVVVVTLDGEQIAANISIQPARVAATAAEGFRQRNFLNTG
                     RAVTA"
     gene            11789..12694
                     /locus_tag="VF516_00220"
     CDS             11789..12694
                     /locus_tag="VF516_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106111.1"
                     /translation="MTDTNIYFGIPGSLVTLPHPRGGQSATRDRPTQTFPLGNGEDRT
                     RRTLHGSRTYVLDWERLDLDTFSTIEAFNQGHMGPGPFAYLDPGQRNQLLVNQAASTS
                     LSNDTSNFSIAGSGCSIASSFTLATGVPRSLAWTFNIANPASGSAILTLDSPYTGWPG
                     VPVVNRAMCFSCLARGGGADAIVSFLLELRWYDTGANLLSTSQGSTLTTSAGAWATGL
                     VTASAPANAAYVLPRVHYLSGASAGAVAYFSSFQLEEGSTRGTWRPGTGVLPVTVVGN
                     EDQWPWLYATEVRERPRLTLRQDGG"
     gene            12698..15721
                     /locus_tag="VF516_00225"
     CDS             12698..15721
                     /locus_tag="VF516_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106112.1"
                     /translation="MQNASASYEDASGAAGGAWAPPKLMIDWMRDGYDVTPNMVDFFN
                     RNLADRFGTTDTGQDWINITGPATAFDVIWDGTDTLVGGYGQHTITEPNDGVSYLTRN
                     SVLNGPKVFVTDQLIKVGIPTPVGGNIEVGMFLRFVDAGNFYRSVAVVDTGGGVNLAI
                     TRSVGFAEDAPVSTSAAVASYTAGQVLAVRTRMLADGTIQSKAWIDGTTQPDWQLTYT
                     DPAQILTPGVAGLRTVVYPGNTTPRPVLIKYYEYRVTNGLPDDVSNQLGGWSVTHHLD
                     DGYPDNVTFIAGVGVPELSADIGAPPAHQTGGVPQQVAEYYSPYNTASPLYGRDRDVA
                     PVTLDHGVITANGPERIRVFTGQMLDLPVRRGKAALSATSRTRLALSSLVQPPPAYGL
                     YQGANATWEISYALAACGVYASPPPQPGCRWWSPQHGSARPFIPSATHRAVDMLVTVN
                     GLSGLRRTLPIEWVTGPFVLGGRYSYNSNWIQGTGSNGATGDGVYLEAGDNLVSSAGL
                     GKVECWVRGDPTELNASPGASGGISTWWRWLMTNTNGSLVDVGITASPGSSDRKPYLN
                     VNDGSLKTFTHSTAVPADGEWHFVGWAWSIAGSKRWINLDGVVETNSTAITAGGYPAQ
                     DTFATNRPFVQMLLPTAEVQVTAGAYGNPDNHPWLRDIPFTPGAVLNPSRLELASIVE
                     PRPREAWELIASFAQAELAALRTDEHDIVTYLTSGWWVKTAQQVVAELVSTETSAGQV
                     DLNIDPTKIRNSIRITFNEAFNVDTWSLVASLRDVITLAPGATTIVVPLNPSAVELRG
                     LTFTNVAAADTTQPLDTNNISINSETNGTGTYATSGEVSALVTEWNSGQAVVVITNTT
                     GATWYTANNKNWQVLGIAAKSVPTSSAFVTDADAATIATRGERVLAIDSQVLQTRVNA
                     RRLARVLKLALRTPTPIMEGLTEFGEARRQPGDLVQFEDPSVTRASGLWRRQSVTHKY
                     SPDGSYTQDTILRPTLPICVVGEGIIGQSLVGPEE"
     gene            15726..16328
                     /locus_tag="VF516_00230"
     CDS             15726..16328
                     /locus_tag="VF516_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106113.1"
                     /translation="MVAYPIIALNDGDILDPQWIDDITDAANDHQTRVTALEQGFNPQ
                     VSYTTNGVMASSTGAEVAMTAWTGGVSSTFLFKAGWVHEMVIQFGGSDSTAGSSAGRQ
                     IARVRKGVNTIVGQQLALMYLSTVGNSGAVTTGSATRYVKNATGADISTQIGMTIQRQ
                     TGAGTITLYGDTDVPLTLAMRPLGLISQLTNFAAVAVSIT"
     gene            16372..17196
                     /locus_tag="VF516_00235"
     CDS             16372..17196
                     /locus_tag="VF516_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106114.1"
                     /translation="MTSRQHAAWIARGKPWKPARPVDRIVTKLRGYGYTVYILGNDAH
                     LDHIPPERHCPYSETNWSAYQNRPMPDVVTAFDTMPRTVGMANRGMPTLQQLGKQMFD
                     DKQAGHSGIRWLIGMNWGPASDRTAVRDTWRPSHVRTTSSDAGHIHGECDSSMITSVV
                     GDDYDPVARFLGQGNTYDEQEEDDMGGFLAQDDKTGAVYHVVNGESFYQTQKALNDKV
                     YLTAEGLYPFDKAPKTHANEWFTYTTNAGSVWIRKGWNAETFGNVAGPASVAAPGN"
     gene            17199..17414
                     /locus_tag="VF516_00240"
     CDS             17199..17414
                     /locus_tag="VF516_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106115.1"
                     /translation="MDLPLDLLGQLGPSGLLAGYIVSQMTGRLISRTVHLDRVNDLKA
                     AIAALEATVAERERQIGILMGRPRDPS"
     gene            17608..17898
                     /locus_tag="VF516_00245"
     CDS             17608..17898
                     /locus_tag="VF516_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106116.1"
                     /translation="MFELLLGGSVLLSLAFVAALGLPWRHHDRAMAWFLSSTGWAAIT
                     IDGALFVAVLGARLPQWVFALALGVQNAVFGWRLWLVVRDRVRWRRIRVEEE"
     gene            17904..18215
                     /locus_tag="VF516_00250"
     CDS             17904..18215
                     /locus_tag="VF516_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106117.1"
                     /translation="MKINFKFQPVAWMSTVLGALTALMTLDETLEQTHAGDIVPTAWE
                     PYVLGAIAVLTAVLGKVVHSKVTPLADPRAANGRPLVPVPPARVPSGASPDALDAERD
                     H"
     gene            18408..19490
                     /locus_tag="VF516_00255"
     CDS             18408..19490
                     /locus_tag="VF516_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106118.1"
                     /translation="MCGKRGRVCLATTHKPAKISNPMRLALQETFQAELGISRSDGTV
                     ARSGIVEAWMIKTSTAVALIDRGLLTIESMAYGEKDAQGYPVRWRKVHQITAAGCAAI
                     GRDMAEIIAWAWEQAVAEYSERCTSPTPEGGYNLTTALPVEVGAVVGWTHKGEAVTGK
                     ITAIFGDDATILGADRTGWTVGLADIERVVELTPTCDHGFPVVEEQPSAFEGAPPPRQ
                     YADGCQSYGPYAELTDQPRYPEGTPEHTAYAAELRSELDKFAKGEPPYEYGPVDKAAI
                     LTKEPIDSLLSGAVEPKSLMALVNDAIAAAVHSEQLTGAPSTIRERSDARAAATAALG
                     AVAERVADLERVRLELAEAEKREISK"
     gene            19487..19819
                     /locus_tag="VF516_00260"
     CDS             19487..19819
                     /locus_tag="VF516_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106119.1"
                     /translation="MITMDWERVESKGALVYWRRRLIDRSYLAVVKQTTGEHAGRWRW
                     QHRGPEIARGTGTVVHAEGFRARAETAQRAADQHVNYRYGIDDTCGTCGVLARECGHR
                     FPETSQEK"
     gene            20061..20501
                     /locus_tag="VF516_00265"
     CDS             20061..20501
                     /locus_tag="VF516_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106120.1"
                     /translation="MTTTPSILPTWPEPDADGAALDLAEVATTLGLSTSAVEVQLKRT
                     RAGTARVAFPEPRGSAHREQPAPGGRPAYARRFWRAADIERYAAERKRGGQRGHRQIP
                     AEEVHRIRRMHAVDGYGVNAIAERTGRTKASVSRILSGKTHREV"
     gene            20498..21130
                     /locus_tag="VF516_00270"
     CDS             20498..21130
                     /locus_tag="VF516_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106121.1"
                     /translation="MMGYPLAVGGDLSLTKSGLAWPDGVVVTHGRDGLTSQRTPLQER
                     GQALMGLVWDLGSLIGCRTLGGDLTGLAETVEWPALVVLEDFPPTSDQRIDPERGYLW
                     WSLVNLLGRFGVPVLPVPPATLKIYACGLGSAHKDTVWAGVAEHFPQYEIHNTHKTTG
                     KVLASKSYDRADAVTLMAIGCDLLGAPLVELPAKHRRALDTLELPPGVKL"
     gene            21157..21282
                     /locus_tag="VF516_00275"
     CDS             21157..21282
                     /locus_tag="VF516_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106122.1"
                     /translation="MVERETFYGDRSQRFTIKIIWLRISFEVRLGLPWRQRWRPW"
BASE COUNT         3576 a         7605 c         7092 g         3080 t
ORIGIN      
        1 cctggctgga gaaggccgat cagcagctca tgcggcgcgc tggcgccggc agtggccggc
       61 gtcggtagct gacctggtcg ggtgcgccgt tcagctcagg ggcgtatcct ccgactcggc
      121 agaccgacca cagccccagg gggatccatg cgcctcgacc tcggcgacgt gcagctcgac
      181 atcaccgcgc acgttcccgg gctgcgtcgg tacggtatcc gcctccacgt gggcgcaccg
      241 cgcgacacca cggcggccga tcgcaccccc cgaggacgcc tcgggccaac cccacgaaag
      301 gtggacttca tcatggatct catggcggac aagcaggtcg acgtcgatgt ccagtggacc
      361 gacgagatcg gccacccggt cgacgcgccg gccggcgcca cggtctcctg gacgaccgac
      421 aacccgacgg tggttgccgt aacccagcac gaggacggca ccgccacggt agcggcgacc
      481 ggcgagctgg gcaccgccaa cgtcagcgtc agcgtctccc acgacggccg caccttcacc
      541 ggtgacgcgc aggtcaacgt cgtggccggc ctcgccgagc gggtcaacat cgtattcggc
      601 gcgccgaccg aggtcacccc ggacgagccg ccggctgagc cggcgccggt gctgtagcct
      661 cacccctcgg gaacgcaacg actgaccgcc tctcatcacc cagaacgccc accggtaccg
      721 gtaccccctt ccggtgggcg ttcgcccatt ccggacaggc ctgcccttgg tttgttagga
      781 actctgccgt actatccgtg atcaggtagt gcgttttgcc cgagacgggg cgcctatctc
      841 gctcgcgacg agcaatcgat gcaagatcca cgattgtgcg cgagcgagag gacagcagat
      901 gacgcgcacc ggctcccgtc tggtttggga cgtccggccc gacctcgacg ggttcgacca
      961 cccgcacctc ctgggcgtca cgccgagtgg ccggccgatc tggtccgcct ccggtggtgc
     1021 gcccatcgac gtctcttcct ggatcccgat cgagtacgac tccgatgtcg tgcagcgggt
     1081 ccggatggaa tcggtcatcg aaggccgcgc ccggcgcgtg gtcatgggct cgaagaccaa
     1141 gtcgattccg cgctcggcgg gcgttaccgt caccgtcggc accacgtaca ccgacgacgc
     1201 gagcaccaac gacgaggtga cgctgaccgc gcggcgcttc atcagccgcg tgcagatcga
     1261 cgaggacgac ctggccgacg cgagcacccg catggacgtg ctcgggacca agtccctgga
     1321 ctgggcgatc tcctacgcgg acaccttcga caatgcttgt ttgggtgtga ccgcgaccga
     1381 aaccggtacc gccacggcgc cgttcacctc ggcgtacaag cggctgcgga gcacggcggc
     1441 cggcctcggg tacacggcgg acgacaacta caccacgtgg gacgacgacc tcatctcgat
     1501 tgcggccacg ccgaacggca cgagcctcta cgagaagctc tcgttcattt tcaagaaggt
     1561 tgagaccaag gcctactggt cccagccgga catgctcgtc atcgcccacc cgggttggcg
     1621 ggacgcgctg cgtctgtgca ccgacgcgca gggccgcccg atcttcacct ccggtggcgg
     1681 cgcgccgggc aacggcaccc ccgacaccct ctttcagacg ccgatcgcat ggtcgcgcgg
     1741 ctgcaagacc tcgccggtgc tccagaccag cccgaccggc aacgacctgc tgtacttcgt
     1801 caacgtgcgc tacctggccc ggggcgaccg ctccggcccc gaggtgctca ccgacgacgc
     1861 acgcgcgcag gacgacaacg acaactacgc catgaagttc agggcgcggc gcggcttcca
     1921 gctcactcac ccgaacgcgg cggccgtcct ggagcggatc accgactaac cggccccagg
     1981 gccgacgcgg acctgcgggt ccctgtacga gcggacgacc ggcggcgagc caacccgccg
     2041 ccggtcgttc ccttccgggg agggtggtgg tcgacgtgac agcggtcgag ctgaatatcg
     2101 accttgccca gaaccgcacg ttcgaactgg tcgcggccgt gctcgccagc gacggcgtca
     2161 ccccccgcga cgacctggcc ggctggaccg gcgccatgca gattcgggac agccgctcga
     2221 cggcggcaac cctgctcgcg gaggcggacg tcaccgtcga tgcgaccacg ggcacggtga
     2281 cggcgagagt gccggcggcc gacaccgacg gcgccacctg gcgctccgga gcctacgacc
     2341 tgtacatcac caacggcacc gcgcgggaag acctcgcata cggtgtcgcc cggctccgcc
     2401 gggccgtaac cgcgtaggga ggccagcgtg gcagccaaga agggcaagaa gaagaccgtc
     2461 cacaccaaga cgtactcggc gatgcgtcgc cggggcgtcg gcaagggcgc cgcgaccaag
     2521 atggcggccc gtgccgcccg gaagtgctga gggacgctgt gggcaagctg ctggtggtcc
     2581 gcaagcgggc cgataggacc gggccggaac cggcccccgg gcagccgtgg ccgctgctcg
     2641 gcgtcgagct ggtcgacgcg ccggacacgt gccgggtgcc gaccaactgg gtgaacaagg
     2701 ccgtcgccga gggctgggcc gaactggtcg ggccggccgt ggtgatgcgt ccgggcggcc
     2761 ccgccagcga ccccgccagc gtgctgcacc agttcctgca cgccgaggcg ctgcgcatcc
     2821 acaccctcga cggccccgtc acctaccgga tcacccgcca gccggacaag tacgtggccg
     2881 gcggcgccga cgacgagccc gtcacggccg aggcgtacgc ggccggggac acgcaggtcg
     2941 accactggta tgagctggag cgcgaggttt gacatggccg acctggtctt caaccgcagc
     3001 aaggggcgcg tggtccagtt cgctgaactg atcaacggca acgccacccc gtacgtgaac
     3061 gcggccttca tcattgaggc gatcaacacc tcggcgaccg acgcgacgct gcgcgacctc
     3121 gacgacttcg ccgcgatcga ggccgatggc aacacggccg aggtcaccaa ctccggctac
     3181 gcccgcaaga cggtcaccga ggccgcctcg gcgatcacga tcacgtacga cgacacgaac
     3241 gaccgcgtgg acgtcgacct tccggaccag acctggaccg ccgtaggcgc gggcaccgcg
     3301 tggacggacc tggtggtcgg cttcgacaac gacaccacgg gcggcaccga ctcgaacatc
     3361 ctgccgtgca cgctgcacga cttcgcgatc accccggacg ggtcggacat caccgcgacg
     3421 atcaacgtgt tctaccgcgc gagctgatcg gggaggcccg ccatgccggc caccctgatc
     3481 ggcagcaaca cctacgcctt cgccgacggc aacgcgggcc atgtcatcga cctgggctca
     3541 tcgcccagcg tcgggcagac cgacgtactg tgcgtcaact ccgacaccgt ggtcaccacc
     3601 ccctcgggct ttgcgctcga cgtgagccgg gtcaactcac agggctcgta catctaccga
     3661 cgcaaggcgg tcggtggcga gggccagacg gtcaccatca ccaccagcgg caacaacaac
     3721 acgtcggtga gttggctgcg ctggggcggc ctcaacgcca aggacgcgac ggccaacagc
     3781 ggtgtcgacg gtgtggccgg taccagttcc ccggcgcaca gcagcggcac catgaccgcc
     3841 tccactgagt tgatcatcgc gttcgccgcg ctgcacagct tcggtgccgt tccctcctcg
     3901 ccggtctggg cgaactcgta caccggacag acagccgtca cccagggatc cggcagcacc
     3961 ggcgccggga acttcgtggg ttacaagcag ccggtcggta cggccgccga gagcccgtcg
     4021 gtgtcctgga ccggcaacgt ctccgaccgg tacatgctga cgatcacgtt cacgatcacc
     4081 ggtggtctgt ccggcaccgc cggcaccgcc accgagaccg acacggcgca gcccatcggg
     4141 aagcgcaaga cccgcgcggt cggcacgacg agcaccacgg acacggcgca gccgatcggc
     4201 cggcgcaaga cccgggcggt cggggttgcc agcgaggcgg acaccgccgg caccctcggg
     4261 aagcggaaga cccgcgccct cgccgtggcc ggcggcaccg acacggccgg cacgatcacc
     4321 cggaccaaga cccgggccgt cgggaccgcc agcgacgcca gcgccaccct cacgctcggc
     4381 cggctcaagg cacgagtggt cggcaccgcg acgaccacgg acacggcgcg cacgctcacc
     4441 gctggccacc tgcgggcgct cggcacggcg gccgaggtcg acaccgcccg gacgatcacc
     4501 accgttcacg ccatcgtccg cgcggtgggc accgcgctgg aggtcgacac cgcgcccccc
     4561 gtcaccgcct cgggtggcct gccggtcatc cgccggccga cgatcgacgt ggtgaccggg
     4621 ccgctgcatg tcgcggtggt cgacgccccg gcggtcgccg tggtggccgg cggagtgcgg
     4681 gtcgtggtgg tcagtgacgg ctgacctggc gggctgtacc ctcggtttgt tcaggtacat
     4741 aacaaagtgg aggctctcat gacggcagca gtcgccgaga tcacggcgac cgacacgtgg
     4801 atcaacgcga gtacgctcag ccggccccag ctcatgaagg cggcggcggc gcgcggcttg
     4861 cagcaggtcg gcacgaacgt cgagctggtc aaccggatca accagtggca gcaggagcag
     4921 ggcctgtacc cgccgccgcc accggacgag gacgagctgg acctggaggt cggcgacgac
     4981 gaggacgacg acgagccggg gccggcgccc gaggccgtcg gcaccgagcg ggcgcccgac
     5041 ccggacgagg acgaggaccc ggagccggca cccctgccga tgccggcggc tccaaccatg
     5101 aagctcccgg ccgccgggaa ggtagcctcc agcccggcgg ccggggtcaa gaagcggcgc
     5161 accggcgagt ttccgagtgg ttggcgcgcg gagtaccgcg tcggctggag cggcatcaac
     5221 gacgagctgc atttccggct catcgaggag acgcaccacc gggccgtcga ggccggctac
     5281 cgcaccaagg gcgcgccgta cgccggcctg cgcatcggct accacgggca gggcgacgcc
     5341 cgcaccgtgg tgtacgaggt cgcgatctcg cgggaggact aatgggtacc tgggcgacca
     5401 tcgaaaccgt atttgcggtg acccgacgtg aggtctccga agacgatctc gcgctcgccc
     5461 agtcgatcat cgaggacttc gcgggcaccg acgtcgactt caccgatgac agcgcgatcg
     5521 gtacccgcaa tcagcgctac ctgcgtaagg cggtctgctg gcaggccgca tggctgtcca
     5581 cccacccgga cgcgctcgac gccatggacg tgacgggcgt gtccggggac ggggtgtcgg
     5641 cgcagcacgc caccgacacg gcggcattca tggcgccgat ggcccgccgg tgcctcaacc
     5701 gggtcacctg gcgcaacggg cctatccgga tccggcccgg ccggcgcgcg ggcgtcgtcg
     5761 actccggcga ccgcgacgac gcggtacgcg acgacgcgtt cgtctggtct ccgctgtcca
     5821 gcgagggctc gccccgcatc ggccacgccg acccgctgag cgagcgctgg gggcaggtgt
     5881 ggcgatgagt gcacgttggc gtaaccgtct gctcgacgcg ggcgtggtgg cctatttcgt
     5941 ggcgctgccg gtggccgcgt tcgtctcgtc ctgggccggc ctcgccctgc tcgcgttctt
     6001 cctggtcgcc accttcccca gcctgttcgc tggcgggctc ccccggcggc ggtcatgacg
     6061 tggcgcgcca cgactaccgt gaccgtcctg cgcggcaccg gtaccgaccg gttcggcgat
     6121 gaggtcgaca ccgacaccgt ggtctatacc cagcgccccg cctcgatcat cgagcagcag
     6181 ccgaactcgc gttcgcggcc ggccgagggc cgcaccgacc aggtacgcgg ctacgcgctg
     6241 cggatgcact cgaacacccg gctactcaag ggcgaccgga tccgcgacga gcgcaccggg
     6301 gacacctaca ccgtggattt cctggccacc ccggcgaacc cggccggcca cacctctcgg
     6361 cgcgcgaccc ttcgtaaggt aacgtaacta accaacaccg ggccgaccgg tgacccccgc
     6421 acaccccctc ccgggccgac cgggacgcac cgccagaccg ccgacgccga ggaggtggcc
     6481 gacatgcccg tgggcagcac ctacgagaac atccgggtca accacggcta cgaggcgtgg
     6541 tttctggcgg tccagggcaa gagcgtgcgg aagctgaccg aggacatcgc cgacgacgcg
     6601 cgggccggct gccccatcga ctccggtgac ctcgtcgagt cgatcggcac ccgctacccg
     6661 ggcggcctgc gcggcatcgt ggtggtcggc accgaccact gggtgcccac cgagtacggt
     6721 tcgccgccgc acatcatcta ttcccatggg ccgtggtcgc tgcgctcgga cgagggtgag
     6781 tacttcgggc gggtcgtgca ccatccgggc accccggcgc agccgttcat gcggccggcg
     6841 ctgtaccagc ggcgtaagcc ggtgacggtg tgaccacccc gctgttgccc cggctggcca
     6901 acaacgagct ggtcgcgctc gcctggatcc gcgacatcgt cagcgtctac aacgtggccc
     6961 tggggaccac cttgcaggga ccggacccgg acacccaggt gctctcctgg ggcgacaccg
     7021 ggttcgtcac cgcctcgacg gtgggcggca cccccaacgg cacggtgccg ctgcgcgagc
     7081 cggttatgga gatcctgtgt cacgccacca acccgggcaa gtcgcggcca ccgtgggggc
     7141 gggccaacgc gatcgccgag acgatcatcg aagcggcccg ctcgatcaac tggggcgaca
     7201 cgcagcggcc ggtcacgctg ccgaccggct acccggcggt gcgggtcggc gacgggtcgg
     7261 cgctcaccga gcccgagcgg cgcaggtcgg acgaggcgaa ctatgcgatc ttcggattca
     7321 cactgtcagt gagctggttg gcgctctaag tcacaagaga agaggacaaa gggcacatgg
     7381 ctaacaagtt ggcctaccaa gtgatcatgg gtgccggctc gctgtactcg ggcctgtacc
     7441 aggcgacgga gccgctcgac caggcggtca acacctcgcc gcaggcgtcc gcgtgggtgg
     7501 atgctggctt caccaacgac ggcgtgacga tcacgctcaa ccaggaattc gccacgatga
     7561 cggtcgacca gatctccgac atcatcgggc gcaagatgac ccagcgtgac gtgcaggtca
     7621 aggcgaacct ggctgaggcg accctggaaa acctcaccat cggcctgaac tcgggcacca
     7681 tctccaccgg ctccggtggc ggggtcaact acaaggtgta caccccggtc ttcaacggtt
     7741 ccgagctgca accgacgtac ttcgcgtcga tcttcgacgg gtacgcgccc gcctcggcgg
     7801 ccggcctgac gaagcggcgc cggttcatcc tgcgccgcgt gctgtccatc gacaacgtcg
     7861 aggtggcgta caagaagggc gatatgaccc tcgtaccggt cacgttcggc tgccactaca
     7921 tcgacaccgt cacggcacca ttcaagatcg tcgacgagac gtaactatcc actgtggact
     7981 ggcatgcgat cgtcggggcg ctcggagtcg cggcgcgggg gtggggaccc ctgctcgttg
     8041 gcttcgcgtt cgggtggtac gcgtgccggg gccgctaccg caaccgcaac cgctggaggc
     8101 tgaccatgac caccccctac acccccggca aggccggtcc gatccggatc aacgtcaagg
     8161 ccaaggccaa gccggaggcc ctggagcgtg agaccttcct gcacctcgac gacccggaca
     8221 ccggcgagtc gaccgaggtc accatcgcga aggaactgct gcccagtcag ggtctgcggt
     8281 tcatcacgct ggtgcgcgac ctcggcgagc tgcgcgcggt cgcgcagatg ttcgacgagg
     8341 tgatcaagcc caagggagtg ctcgacaggc tcgccaagat cgagggcatc acacaggggg
     8401 agatcgacga catcatgtcc gtggtcacga ccaagctcat ggcggcggcg gaggcggcgc
     8461 agggaaactg atcagccgag tggcccaggt cggatgggtg ctcgaacacc tcgacgacct
     8521 ggagtcagac ttctcggcca tccaccacat ccacatcgac ttcgaagcgg agcagtacgg
     8581 cggactcagc tcgcggcggt tcttcgccct cgccgggcgc atcatggcct acccgggcgt
     8641 ggtgcaggcc cggtacatgg ccgaggaaca gcgccggcag gaagaccacg agggggtacc
     8701 gatcaccgac acggaccggc cacggaccgc gccgagggcg gaacccggcg tgaaggtcat
     8761 cccgctgacg ccggaactgc tcgccaacgg agtcgacgcg ctcggactga gcggcgtcgt
     8821 cgaatacgcc acctcgggct gagggaaggg ggtagggcat ggcgctcggg taccgcatcg
     8881 ccgacggata tatgcgggtc gatgccgacc acagccccgc cgatcgttcc ctcgccgggt
     8941 tcttccgggc cgctgacggc cggctgcgcg acatgcgcgg ccggttcgcc gccgagggcc
     9001 agcttgccgg ccaggagtac acgaagcgct tccaggaggc ggccaagaac cagaaacagt
     9061 ccggcttcga aggctttgcg cgcaagctcc tgggccgctt cggggcgctg ggcaaggctg
     9121 ccggcggact gctcgcgaac aagatggtgc tcgccgccgt ggccggcctc ggggcgctgc
     9181 ctacggcgat cgcggccgtc ggccaggccg tgggcggcct gctgaacgtg gccggtgccg
     9241 gcgccgcgct cgcgccggcc ggcatcgccg ccgccgcgtt cagcctcatc acgctcaagg
     9301 tggcgctgtc cggggtcggc gccgcgttca aggccggcct ctccggcgac gcggagaagt
     9361 tcgccgaggc gctcaagaag ctggcgccgg ccgcgcagga ggccgtcaag cagctcgtgg
     9421 cgctggcgcc ggccgcgcgc cagctcaaac aaaccgttca gaacagcctg ttcgcgccgt
     9481 ggctcgacga catcaagcca ctggcaagca tctatctgcc gatcttgacg caggagatga
     9541 cctggcttgc cgccgccgcc gggatggcgt tccatcacgt cctggagctg ttcgagacgc
     9601 cggccgccgc ccaacagttc cacgacatgc tgaccggctt cgtgcaggcg ttctcgaacg
     9661 tcgccgccgg cctgcccggc attgtgtccg gcttcttcca gctcgcctcc gccgggtcgc
     9721 tgttcctggg ccggttcacc gaaggattcg ccgggctgac tcagcgcttc gccgagtggg
     9781 cgacggcgtt cacccagtcg ggccgcttcg atgcgttcgt cgagcacgcc ctggagaccc
     9841 tgggcgggct cagtcgagcc ctccaggaca tcgtgggtat cttccagtcg ttcaaccggg
     9901 ccgcgcaggc ggtggtcggc ggcggcctgt tccaggcgct tggccagatc ctggacatgg
     9961 tcaaccggtt cttccagtcg gtgcagggcc aggaggtgct caccgcgctg ctgaccaagc
    10021 tccagacgct gggcggcatc gtcatggatc tggtgtccgg cgcgctgccc ggcctgctgt
    10081 cgctgtccgg cgcgctgctg gaaggcctcc aggcgctggc gccggtggcc ggccccgtcg
    10141 gcgaggccct tggcgcggcc ctgtcggcgc tggcgccgat cctgcccgtc ctgggtagct
    10201 tgctggcccc cgtgctgaag ctcgcggccg gcgccctcca gagcctggcc accgtgctcg
    10261 ccccggtgat ccaactcttc tcggagctgg cgacacaggc cctgccgctg ctcatgccgc
    10321 tgatcgacga actggtggcc aacgccctac cgatggccgc cgccctcgcg accgagctgt
    10381 acaacgcgtt cgcgccgctc gtgccggtgt tgctagaggt gtaccacgcg atcgcggacg
    10441 ggttgatggc agccttgccc gacctgctcg aaaccaacaa ggaattgatc cctgtcgttg
    10501 gggagatcgc caagatcttc gccgatacct ggctgtcggt ccttcaggaa ctgcgcccgc
    10561 acatcccgat catcattcag ttcttcgtcg ccctgctcaa ggtctcgatg gcgatcggac
    10621 tcacctacct gaagatcatt ccgtacgtgg cgacattcgc gtcatggttc atcaaggccg
    10681 ccgcctgggt ggccggattc gtattgacga ttatcgagat ccctacccac ctgcgggcga
    10741 ttatcggagt cctcaaggaa tgggtgtccg gggcggtctc ggccgtcggc gacttcttcg
    10801 gcatgatcgg cggctggatc tcgcaggcgg cccagtggtt cgccgacctg cccggtaaga
    10861 tcatcggctt cttgcagcag atcccccagg ccgtgcggga tgcgctatcg gcagcgttcg
    10921 acgccttctt ctactggacg ggcttcatcg cggccaaggt ggtcgagttc gtccagtcga
    10981 tcccgctgcg tatcgccatg atgtggcaga ccatcaaaac gacgttcatg gatggcgtct
    11041 cggccgtcgg aaacttcttc gctgagctgc ccgtcaaggc cgaattcttc ttttccgaca
    11101 tgtacaacaa ggtgaccacg tgggtggtca acacgtacaa ctccgtcggc cagtggctgg
    11161 gctcgctgcc gacgcgggcc ggtaatgccc tgtcaagtct ctggtcttcg atccggggcg
    11221 cgttcgacac cgccacgacg aatgtacgta aggcgatcgg cgaactgatc gacggcgcgg
    11281 tcaccctcgc caagagcctg cccggcaaga tccgcgacgc ggtgggcaac ctcggtcacc
    11341 tgctgtacga cgcgggccgg gacatcgtgc acggcctcat cgacggattc aactccatga
    11401 tcgactgggc gaaaaacctg gtcggcaacg ccatgaagaa gatcaaggac ggcgccaagg
    11461 cggccatcgg ctccaactcg ccctcgaaag ccttccgcga cgaggtgggt aagaccatcc
    11521 cccagggcat cgccgccggc atccgggccg gcatgccgga cctggagcgc tacctgacca
    11581 tgcgggtcgc cgcgctgccc ggcatgagca gcgccaacgt taacgtggcc gcccccaacg
    11641 tcgccgtcgg agccccggtc gtcgtcgtca ccctcgacgg ggaacagatc gccgccaaca
    11701 tctcgatcca gccggcgcgg gtcgccgcga ccgccgccga gggcttccgg cagcgcaact
    11761 tcctcaacac cggacgggcc gtgacggcat gaccgacacc aacatctatt tcggcatccc
    11821 gggcagcctc gtcaccctgc cccacccgcg cggcggacag tccgccaccc gcgaccggcc
    11881 cacccagacg ttcccgctcg gcaacggcga ggaccgcacc cggcgcaccc tgcacggcag
    11941 ccgcacctac gtgctcgact gggaacgcct cgacctcgac accttctcga cgatcgaggc
    12001 gttcaaccag ggacacatgg gacccggtcc gttcgcctac ctcgaccccg gccagcgcaa
    12061 ccaactgctg gtcaaccagg ccgcctcgac gagcctgtcc aacgacacga gcaacttcag
    12121 catcgccggg tccggctgct cgatcgcctc gtccttcacc ctcgccaccg gggtgccgcg
    12181 ctccctcgcc tggacgttca acatcgccaa cccggccagt gggtcggcca tcctcaccct
    12241 cgacagcccg tacaccggat ggcccggagt cccggtcgtc aaccgggcga tgtgcttctc
    12301 ctgtctcgcc cggggcggcg gtgccgacgc catcgtgtcg ttcctgctgg agctgcgctg
    12361 gtacgacacc ggggcgaacc tgttgtcgac ctcgcagggc tcgacgctga ccacctcggc
    12421 cggcgcctgg gccaccggcc tggtcaccgc gtccgcaccg gccaacgcgg cctacgtgct
    12481 gccccgcgtg cactacctct cgggcgccag cgcgggcgcg gtcgcctact tctccagctt
    12541 ccaactggaa gagggttcga cgcgcggcac ctggcggccc ggcacgggcg tgctgccggt
    12601 gacggtggtc ggcaacgagg atcagtggcc gtggctgtac gccaccgagg tgcgcgagcg
    12661 gccccggctc acgctgcgcc aggacggggg gtgagccgtg cagaacgcga gcgccagcta
    12721 cgaggacgcc tccggcgcgg ccggcggtgc ctgggctccg cccaagctca tgatcgactg
    12781 gatgcgggac gggtacgacg tcacgcccaa catggtcgac ttcttcaacc gcaacctcgc
    12841 cgaccggttc ggcaccaccg acaccggcca ggactggatc aacatcacgg ggccggccac
    12901 cgcgttcgat gtcatctggg acggcaccga cacgctcgtg ggtggctacg ggcagcacac
    12961 catcaccgag cccaacgacg gcgtcagcta cctgacccgt aacagcgtgc tcaacggccc
    13021 gaaggtgttc gtcaccgacc agttaatcaa ggtcgggatt ccgaccccag tcggcggcaa
    13081 catcgaagtc ggcatgttcc tgcggttcgt ggacgccggc aacttctacc gctcggtcgc
    13141 ggtggtcgac accggcggcg gggtcaacct cgccatcacc cgctcggtcg ggttcgccga
    13201 ggacgcgccg gtgagcacgt cggcggccgt ggccagctac accgccggcc aggtgctggc
    13261 ggtgcgcacc cggatgctcg ctgacggcac catccagtcc aaggcgtgga tcgacggcac
    13321 cacccagccg gattggcagc tcacgtacac cgaccccgca cagatcctga ccccgggagt
    13381 ggccggcctg cgcacggtgg tctacccggg caacaccaca ccccgccccg tattgatcaa
    13441 gtactacgag taccgggtga ccaacggact gcccgacgac gtgtccaacc agctcggcgg
    13501 ctggtcggtg acccaccacc tcgacgacgg atacccggac aacgtgacct tcatcgccgg
    13561 tgtcggggtg cccgagctgt ccgccgacat cggcgcgccg ccggcccacc agaccggcgg
    13621 agtgccccag caggtcgccg agtactacag cccgtacaac accgcctcgc cgctgtacgg
    13681 ccgcgaccgg gacgtggcgc cggtgaccct cgaccacggc gtcatcaccg ccaacggccc
    13741 ggaacgcatc cgggtgttca ccggccagat gctggaccta ccggtgcggc gcgggaaggc
    13801 cgccctgtcg gccacctcgc ggacccggct cgccctgtcg tcgctcgtgc agccgccgcc
    13861 ggcctatggg ctgtatcagg gcgccaatgc cacctgggag atcagctacg cgcttgccgc
    13921 gtgcggggtg tacgcctcgc cgccgccgca gcccggctgc cgctggtgga gcccacagca
    13981 cggctccgcg cggccgttca tcccctcggc cacccaccgg gcggtcgaca tgctcgtcac
    14041 tgtcaacggg ctcagcggcc tgcgccgcac cctgccgatc gagtgggtga ccggcccctt
    14101 cgtgctcggc ggcaggtact cctacaacag caactggatt cagggcaccg gcagcaacgg
    14161 cgccaccggc gacggggtgt acctggaggc cggggacaac ctcgtcagct ccgccggtct
    14221 gggcaaggtg gaatgctggg tgcgcggcga cccgaccgag ctgaacgcct cccccggggc
    14281 cagcggcggc atcagcacct ggtggcgctg gctgatgacc aacaccaacg gcagcctggt
    14341 tgatgtcggc atcaccgcct cgcccggctc cagcgaccgc aagccgtatc tcaacgtgaa
    14401 cgacgggtca ctgaagacct tcacccactc gacggccgtt cccgccgacg gcgagtggca
    14461 tttcgtcggt tgggcctgga gcattgctgg ctccaagcgc tggatcaacc ttgacggcgt
    14521 ggtcgagacg aactcgaccg cgatcaccgc cggaggctat ccggcgcagg ataccttcgc
    14581 cacgaaccgg ccgttcgtgc agatgctcct tccgacagcc gaggtgcagg tgacggccgg
    14641 cgcctacggc aacccggaca accatccatg gttgcgagac atcccgttca cgcccggggc
    14701 ggtcctcaac ccgagccgcc tggagttggc cagcatcgtc gagccccggc cacgggaggc
    14761 ctgggagctg atcgcctcgt tcgcgcaagc ggagctggcc gcactgcgca ccgacgagca
    14821 cgacatcgtc acctacctga catccgggtg gtgggtgaag accgcgcagc aggtggtcgc
    14881 cgaactggtc agcaccgaga cgagcgccgg ccaggtcgac ctgaacatcg accccaccaa
    14941 gatccgcaac agtatccgga tcacgttcaa cgaagcgttc aacgtcgata cctggtcgct
    15001 cgtggccagc ctccgggacg tgatcaccct cgccccgggc gcgacgacca ttgtggtccc
    15061 gctcaacccg agcgcggtcg agctgcgcgg cctcaccttc accaatgtgg ccgccgccga
    15121 caccacgcag cccctcgaca ccaacaacat ctcgatcaac tccgagacga acggcaccgg
    15181 cacctatgcg accagcggtg aggtcagcgc cctggtcacc gaatggaact ccgggcaggc
    15241 ggtcgtcgta atcaccaaca ccacgggcgc cacctggtac accgccaaca acaagaactg
    15301 gcaggtgctc ggcatcgccg ccaagagcgt gccgacgtcc tcggcgttcg tcaccgacgc
    15361 cgacgcggcg accatcgcca cgcgcggcga gcgcgtgctg gcgatcgact cgcaggtcct
    15421 acagacccgg gtcaacgcgc gccggctggc ccgggtgctg aagctcgcgc tgcgcacacc
    15481 gaccccgatc atggaggggc tgaccgagtt cggcgaggcc cgccgacagc ccggagacct
    15541 cgtccagttc gaagacccca gcgtgacccg ggcgagcggc ctctggcgcc gccagagcgt
    15601 gacccacaag tacagcccgg acggttcgta cacgcaggac accattcttc ggcccacgct
    15661 gccgatctgc gtggtcggcg agggcatcat cggacaaagt ctcgtagggc cggaggagta
    15721 gccccgtggt cgcgtacccc atcatcgcgt tgaacgacgg cgacatcctc gatccacagt
    15781 ggatcgacga catcaccgat gcggccaacg accatcagac ccgggtcacc gccctggagc
    15841 agggcttcaa cccgcaggtg tcgtacacca ccaacggcgt catggccagc tcgaccggcg
    15901 ccgaggtggc catgaccgcc tggaccggtg gcgtgtcctc gacgttcctg ttcaaggccg
    15961 gctgggtaca cgagatggtg atccagttcg gtgggtccga cagcacggcc ggcagttcgg
    16021 cgggccggca gatcgcccgc gtgcgcaagg gcgtcaacac catcgtgggg cagcagctcg
    16081 cgctcatgta cctttccacc gtcggcaaca gcggcgccgt gaccacgggg tccgccacgc
    16141 ggtacgtcaa gaacgcgacc ggcgccgaca tcagtacgca gatcggcatg accattcagc
    16201 ggcaaaccgg cgccggcacc atcacgctct acggcgacac cgacgtaccg ctcaccctgg
    16261 caatgcgccc gctcggcctg atctcgcaat tgaccaactt cgccgccgtc gcggtatcga
    16321 tcacgtgact acgtaatgtc atgatgggtc gcagcagggg agggggttat tttgaccagt
    16381 cgccaacacg cggcgtggat cgcccggggg aagccctgga agccggcgcg acccgtcgac
    16441 cgcatcgtta ccaagctgcg cggatacggg tacacggtgt acatcctggg caacgatgcg
    16501 cacctcgacc acatcccgcc ggaacgacac tgcccctatt cggaaacgaa ctggtcggca
    16561 taccagaacc ggccgatgcc cgacgtggtg accgcattcg acaccatgcc gcgtaccgtc
    16621 ggtatggcga accggggcat gccgacgttg cagcagctcg gcaagcagat gttcgatgac
    16681 aagcaggccg gccactccgg gattcgctgg ctcatcggta tgaactgggg tcccgcctcg
    16741 gaccgtaccg ccgtacgcga cacgtggcgg cccagccacg tgcgcacgac atcgagcgac
    16801 gccggtcaca ttcacggcga atgcgactcc agcatgatca cgtcggtggt cggtgacgat
    16861 tacgacccgg tggcgcggtt cctggggcag ggcaacacgt acgacgagca ggaggaagac
    16921 gacatgggcg gatttctcgc gcaggacgac aagaccggcg cggtgtacca cgtggtcaat
    16981 ggggagagct tctaccagac ccagaaggcg ctcaacgaca aggtgtacct caccgccgag
    17041 ggtctctacc cgttcgacaa ggcgcccaag acgcacgcga acgagtggtt cacctacacc
    17101 accaacgccg gttcggtgtg gatccgcaag ggctggaacg cggagacctt cgggaacgtg
    17161 gcagggccgg ccagtgtcgc agctccaggc aactgagcgt ggatcttccg ctcgacctgc
    17221 tcggccagct cggcccctcg gggctgctgg ccggctacat cgtctcccag atgaccggcc
    17281 ggctgatctc gcgtaccgtc cacttggaca gggtcaacga tctcaaggcc gcgatcgcgg
    17341 cgttggaggc gaccgtggcc gagcgcgagc gacagatcgg catcctcatg ggccgaccac
    17401 gggatccctc gtgaggtggc cgcgctggct acgtctacga cgccccaacg gcgacgccaa
    17461 ggaggcgcgt gagcgcgccg aggagcggtt gcgtgccgcc cgccggcagg ccgggcaggt
    17521 gtgccgggag gcggcgaagc cgcttccgga aggggagttt gtcgatcggg tgagcaaggc
    17581 gtttcgtctg gggccttcgt gagactgttg ttcgagctgc tgctgggcgg ttcggtgctg
    17641 ttgtcgctgg cgttcgtggc cgccctcggg ctgccgtggc ggcatcatga ccgggcaatg
    17701 gcgtggtttc tgtcatcgac cgggtgggcg gctatcacga tcgacggtgc gctgttcgtc
    17761 gcagtcctgg gcgcccggtt gccgcagtgg gtgttcgcgc tcgcgctcgg ggtgcagaac
    17821 gcggtgttcg gttggcggtt gtggctggtg gtccgggatc gcgtgcgctg gcggcggatc
    17881 cgggtcgaag aggagtgaga accgtgaaga tcaatttcaa gttccaaccg gtggcctgga
    17941 tgtcgaccgt cctgggcgcc ctgaccgcgc tcatgacgct cgatgagacc ctggagcaga
    18001 cgcacgccgg ggacatcgtg ccgaccgcct gggagccgta cgtcctcggg gctatcgctg
    18061 tcctgacggc cgtactcggc aaggtggtcc acagcaaggt gaccccgctc gccgacccgc
    18121 gcgccgccaa cggccgcccc ctggtgccgg tgccgccggc tcgggtaccc tcgggcgcca
    18181 gtcccgacgc gctcgacgcg gagcgggacc actaggccaa tcgaagcgcc cgagcgcgtc
    18241 ggttgttggg ggatgggtag gagcgccccg gggtgagcac ccggggcgct cgcctatttg
    18301 acacatcgcg tgttcctctg taagctggac acagtccagc gaacatcccc caacccaggg
    18361 agcacgtcat gacacagacg accaccgaaa acacctccgc gccgtgcgtg tgcggcaagc
    18421 gcgggcgagt ctgcctggcc actacgcaca agccggccaa gatctccaac ccgatgcgtc
    18481 tggccctgca agagaccttc caggccgagt tgggcatctc gcgcagcgat ggcaccgtgg
    18541 cccggtccgg catcgtcgag gcctggatga tcaagacgag tacggcggtc gcgctgatcg
    18601 accggggcct gctcaccatc gagagcatgg cgtacggcga gaaggacgcc caggggtacc
    18661 cggtccggtg gcgcaaggtc caccagatca ccgccgccgg ctgcgcggcc atcggccgcg
    18721 acatggccga gatcatcgcg tgggcctggg agcaggccgt ggccgagtac tccgaacgct
    18781 gcacgtcgcc gacgcccgag ggcgggtaca acctgacgac cgcgctgccg gtcgaggtcg
    18841 gcgcggtggt cggctggacg cacaagggcg aggcggtaac cggcaagatc accgcgatct
    18901 tcggcgatga cgccaccatc ctcggcgcgg accgcaccgg ctggaccgtc ggcctcgccg
    18961 acatcgagcg tgtcgtcgag ctgacgccga cgtgcgacca cggctttccg gtcgttgagg
    19021 agcagcccag cgcattcgag ggtgccccgc cgccgcgcca gtacgccgac ggatgccagt
    19081 cctacggccc gtacgcggag ctgacggacc agccgcgcta ccccgagggc acccccgagc
    19141 acaccgcgta cgcggcggag ctgaggagcg agctggacaa gttcgccaag ggcgagccgc
    19201 cctatgagta cggccccgtc gacaaggccg ccatcctgac caaggagccg atcgactcgc
    19261 tgctcagcgg cgccgtcgag cccaagagcc tgatggcgct cgtcaacgat gcgatcgccg
    19321 ccgccgtcca cagcgagcag ttgaccggcg caccctccac catccgggaa cgcagcgacg
    19381 cccgcgccgc cgccacggcc gccctggggg cagtcgccga gcgggtcgcc gacctggagc
    19441 gggtgcgcct cgaactcgcc gaggccgaga agcgggagat cagcaagtga tcacaatgga
    19501 ttgggagcgc gtcgagtcga aaggcgccct ggtgtactgg cgacgtcgac tcattgatcg
    19561 cagctatctc gctgtggtca aacagaccac cggcgagcat gccggccgct ggcgctggca
    19621 gcaccgaggc ccggaaatcg cacgtggcac cggcactgtc gtccacgcgg aagggtttcg
    19681 cgcacgtgca gagaccgcac agcgtgcagc cgatcaacac gtcaactacc gctacggaat
    19741 cgacgatacg tgcggcacat gcggcgtgct ggcgcgcgaa tgtggccacc ggttccctga
    19801 aacgagtcag gaaaaatgat caacgttgct ctcatcacct tgcggggggt cgcgctcgcg
    19861 gccccccgcg cgttcttcac ggcgatgcgc gggctcccgt ccgcgcatcc gccggacgtg
    19921 ccgcaccggt acgaccagcc cccgaacgtc ctgaccatgc acagccgcta cggctggcac
    19981 tgcgtgtgca acggcacgca cccggagccg tgctggtgta acacgctcac tgccgttccc
    20041 gtcatccccg ggagggagac atgaccacca cccccagcat cctgccgacc tggcccgaac
    20101 ccgacgccga cggcgccgcg ctcgacctcg ccgaggtcgc gaccaccctc ggactgtcga
    20161 ctagcgccgt cgaagtgcag ctcaagcgga cccgtgctgg taccgcccgg gtcgcgttcc
    20221 cggagccgcg cggtagcgcc catcgcgagc agcccgcccc cggtggccgg ccggcgtacg
    20281 cccgccgctt ctggcgggcc gccgacatcg agcggtatgc cgccgaacgc aagcgcggcg
    20341 gccagcgcgg acaccgtcag atcccagccg aggaggtgca ccgcatccgc cgcatgcacg
    20401 ccgtggacgg ctacggcgtg aacgcgatcg ccgaacggac cggccgcacc aaggcgtccg
    20461 tctcgcggat cctcagcggc aagacccacc gggaggtgtg atgggctacc cactcgccgt
    20521 aggtggtgac ctctccctga ccaagtccgg gctcgcctgg cctgacggcg tcgtcgtcac
    20581 ccacggccgg gacggactca ccagccagcg cacgcccctc caggagcgcg gccaggcgct
    20641 catgggcctg gtgtgggacc tggggagcct catcggttgc cggacactcg ggggcgatct
    20701 gacgggcctc gccgagaccg tcgagtggcc ggccctggtc gtcctggagg acttcccgcc
    20761 caccagcgac cagcgcatcg accccgagcg cggctacctg tggtggtccc tggtcaacct
    20821 gctcggccgt ttcggtgtgc cggtgctgcc ggtgccgccg gccaccctga agatctacgc
    20881 gtgcggactc ggcagcgccc acaaagacac cgtctgggcc ggcgtcgccg agcacttccc
    20941 gcagtacgag atccacaaca cgcacaagac cacgggcaag gtgctggcca gcaagagcta
    21001 cgaccgggcc gacgcggtca ccctgatggc gatcggctgc gacctgctcg gcgcaccgct
    21061 cgtcgagctg ccggccaagc accgccgcgc cctcgacacg ctcgaactcc cgccgggggt
    21121 gaagttgtga tcagtggcgg tctggccccg atgttcatgg tcgagcgcga gaccttctac
    21181 ggcgaccgct cgcagcggtt cacgatcaag atcatctggt tgcgtatcag cttcgaggtg
    21241 cgcctggggc tgccctggcg gcagcgctgg cgcccctggt agagaggagt ccgacatgga
    21301 tgagatctgg acttgcgcgg tcatcgtgct cgtgttcctc gtgctggtgc tgg
//
LOCUS       DASVOR010000010         1671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58501,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000010 DASVOR010000000
VERSION     DASVOR010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58501"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1081)
                     /gene="ltrA"
                     /locus_tag="VF516_00280"
     CDS             complement(<1..1081)
                     /gene="ltrA"
                     /locus_tag="VF516_00280"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX8106123.1"
                     /translation="MTLAHHIDVDWLREAFRRTRKDGAPGIDGQSAAAYAEHLEANLQ
                     SLLNRAKSGQYRAPPVRRVHIPKGDGTETRPIGIPTFEDKVLQRAVAMVIGEVYEQEF
                     HDFSYGFRPGRSQHQALRAVQNAAWRMAGGWIVEVDIRKFFDTVEHSHLREILRRRIG
                     DGVLLRLIGKWLNAGVLEGLVLSYPDEGTPQGGVISPLLANIYLHDVLDEWFVRDVKP
                     ALAGQAELFRFADDAVALFASKQDAERFLEVLPKRFGKFGLTLHPDKTRMVPFQRPDR
                     VDNDDDHPGTFNFLGFTHYWGRSRKPAPKGRPARRGWFAMQKTAKDRLSRTLRRLYEW
                     CRWHRHDPLKTQHLMLVKKLKGHYGY"
BASE COUNT          245 a          597 c          502 g          327 t
ORIGIN      
        1 agtagccgta gtggcccttg agcttcttca ccagcatgag gtgctgcgtc ttcaacgggt
       61 cgtgccggtg ccagcgacac cactcgtaga gccggcgtag cgtgcggctg agccgatcct
      121 tggccgtctt ctgcatcgcg aaccaccccc tcctggcggg ccgtcccttg ggcgcaggct
      181 tgcgggaccg cccccagtag tgggtgaacc cgaggaagtt gaacgtcccg gggtgatcgt
      241 cgtcgttgtc cacgcgatca ggcctctgga acggcaccat ccgggtcttg tcgggatgca
      301 acgtcaggcc gaacttgccg aatcgcttcg gcaggacctc gaggaaccgc tcggcatctt
      361 gcttgctggc gaacagcgcg acggcgtcgt ccgcgaaccg gaacagctcc gcctgacccg
      421 cgagggccgg cttcacgtcc cggacgaacc actcgtcgag cacgtcgtgc aagtagatgt
      481 tcgccaggag gggcgagatc acccctccct gcggtgtccc ctcgtcgggg tacgagagca
      541 cgagcccctc gagaaccccg gcgttcagcc actttccgat caaacgcagc agcactccgt
      601 cgcctatccg tcggcgaagg atctcccgga ggtgagagtg ctcgacggtg tcgaaaaact
      661 ttcggatgtc gacctcgacg atccaccctc cagccatccg ccacgccgcg ttctgcaccg
      721 cgcgcagcgc ttggtgttgc gaccgccccg gccggaagcc gtacgagaag tcgtgaaact
      781 cctgctcgta gacctcgccg atcaccatcg ccaccgcgcg ctgcaggacc ttgtcctcga
      841 aggtcggaat gccgatcgga cgggtctccg tcccatcgcc cttcgggatg tgcacccgcc
      901 gcaccggcgg cgcccggtat tgaccggact tcgcgcggtt gaggagcgac tgaaggttgg
      961 cctccaggtg ctccgcgtac gcggccgcgc tctgtccgtc gatgcctggg gcgccgtcct
     1021 tccgcgtgcg gcgaaaggcc tcgcgtagcc agtccacgtc gatgtgatgc gccagcgtca
     1081 tcagcctggc gcctcgaagc tccctcgcca gtttcgctat tcgcacgagc ttggttgaga
     1141 cgtcctgcga gctcgatgtc tcggtcatct atccctcctg cgagtctgtt ctccggcgcg
     1201 ctcctttgct cagcagggtc ccctggagcg ggttccccca cgtcacagct cctactgcgc
     1261 gctccgacct ctcggcgccc ggccggctcg atttctcttg ccgttccgtt cctgtccctg
     1321 acaggagcgc cgagactcca tcccaaggtt cctgggcgac ccttgcgtac gtgccccgtc
     1381 ctacgatccc gatgggggca gtggttgaga ccgcggcgct cggcttcccc ggtcgcgcat
     1441 caccacatcc tgtcgccttc gacccgtacg agaggtctcg gcacccactt gttaggcatt
     1501 tcgggaccca acctcgcggc ccgcacgctc gctgtctacg cttcgagatc acggtcgccc
     1561 gtgtacgtcc ttacgatctc gcaagactcg cttccggcgt ggcggtctcc gtcgtcgccg
     1621 gtgggatttt caccccgggt cgctctccga aatttcaggc tgctacatgc c
//
LOCUS       DASVOR010000011         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_29302,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000011 DASVOR010000000
VERSION     DASVOR010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29302"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..693
                     /locus_tag="VF516_00285"
     CDS             127..693
                     /locus_tag="VF516_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEX8106124.1"
                     /translation="MPAASTGPGDHLADNIKALRLARGLSQQQIAKAAGVPRATWTHL
                     ESGAGNPTLAVLVKIAAALQVRLDELLAAPSAPARHIAASELPVRQRGEVTVRKLLPE
                     SLPGLDLERMVLPPGGRMTGVPHTPGTREYLTCERGTVELAVAGERYRLAEGDVVTFR
                     GDQRHGYHNPGTQTAVAYSAIAFAPLTA"
     gene            complement(722..>1546)
                     /locus_tag="VF516_00290"
     CDS             complement(722..>1546)
                     /locus_tag="VF516_00290"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8106125.1"
                     /translation="CVPDLLTAGHLRELLPFLVQPAARLRRLVPVAIRHRIWQHRTLQ
                     AVRELPPAALRRLLADIESTIDVERLRWLRRAHLRAPEELQELCDYRRKLEIAIVKAL
                     LVRLHDGPRRRILDLGSGGGYFVAVCRHLGHSCDGTEVPASRLSSATAVSYAAVRAAL
                     RIGPTIDLAIAANTPIEVPAIEPRSYDLITAHKICFNGHMRPKVWSTPEWRFFVTDVC
                     RFLAPGGQLVLELNEDVARYGSRRWYDAELAHYFGTVGQVVRGWITVERDRVDARS"
BASE COUNT          231 a          593 c          523 g          199 t
ORIGIN      
        1 agccatccgg cgaaccgcgc ccgccaaccg gttggcgacc tgccatcggg tcgaccgctc
       61 gcgcgcacgc cgcggccatc gccctcgcca atctgtcgtc ctatcggcga tcttcttggc
      121 caggccatgc ccgccgcgtc caccggtccc ggcgaccacc tcgccgacaa catcaaggcc
      181 ctgcgcctcg cgcgcgggct gtcgcagcag cagatcgcca aggccgccgg cgtgccgcgc
      241 gcgacctgga cccacctcga gtccggcgcc ggcaacccga cgctcgccgt gctggtcaag
      301 atcgccgcgg cgctccaggt ccggctcgac gagctgctcg ccgcgcccag cgccccggcg
      361 cgccacatcg cggcgtccga gctgccggtg cgccagcgcg gcgaggtcac ggtgcgcaag
      421 ctgttgcccg agtcgctgcc cggcctcgac ctcgagcgca tggtgctgcc gcccggcggc
      481 cggatgaccg gcgtgccgca caccccgggc acccgcgagt acctgacctg cgagcgcggc
      541 accgtcgagc tcgcggtcgc cggcgagcgc taccggctgg ccgagggcga cgtcgtgacg
      601 ttccgcggcg accagcgcca cggctaccac aacccgggca cgcagaccgc cgtcgcgtac
      661 tcggcgatcg cgttcgcgcc gctgaccgca tagccgcggc gcgctgcacg cactcggttc
      721 atcacgatcg agcgtcgacg cgatcgcgtt cgaccgtgat ccagcctcgc acgacctggc
      781 cgaccgtgcc gaagtagtgc gccagctcgg cgtcgtacca ccgccgcgag ccgtagcgcg
      841 ccacgtcctc gttgagctcg aggacgagct gcccgcccgg cgcgaggaag cggcagacgt
      901 cggtcacgaa gaaccgccac tccggcgtcg accacacctt cggccgcatg tgcccgttga
      961 agcagatctt gtgcgcggtg atcaggtcat acgaccgcgg ctcgatcgcc gggacctcga
     1021 tcggcgtgtt cgcggcgatc gccagatcga tcgtggggcc gatgcgaagc gcagcgcgca
     1081 ccgcggcata ggacaccgcc gtggcggacg acaggcggct cgcgggcacc tcggtgccat
     1141 cgcacgagtg accgaggtgc cggcacaccg cgacgaagta gcccccgccg gatccgagat
     1201 cgaggatgcg tcgtcgcggt ccgtcgtgga gccgcaccag cagtgccttc acgatcgcga
     1261 tctcgagctt gcggcggtag tcgcacagct cctggagctc ctcgggggcg cgcaggtggg
     1321 cgcgccgcag ccagcgcaat cgttcgacgt cgatcgtcga ttcgatgtcc gcaagcaacc
     1381 gccgcagcgc ggccggcggc agctcgcgca ccgcctgcag cgttcgatgc tgccagatgc
     1441 gatgccggat cgccaccggc accagccgcc ggagccgggc cgccggttga accaggaacg
     1501 gcagcaactc gcgaagatgt ccagcggtca gcaagtctgg cacgca
//
LOCUS       DASVOR010000012         2343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_380028,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000012 DASVOR010000000
VERSION     DASVOR010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2343
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380028"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(132..929)
                     /locus_tag="VF516_00295"
     CDS             complement(132..929)
                     /locus_tag="VF516_00295"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8106126.1"
                     /translation="MDQVLVEFSGPSGGDLATVILNRPERRNSLSESMLRDLGAALQR
                     AAEPASVRAIIIAGAGPVFSAGHDFGDMIERDLDGMRRLLSLCTEVMLGIARLPVPVV
                     ACVQGLATAAGCQLVASCDLAVAVEDAAFSTPGGRGGWFCHTPMVAVARAIGARRALE
                     LAMTGDAIDARTALAWGLVNRVVPRADLVAETHKLARAASRGSLASKALGKRTFYRQI
                     DLDVEAAYAEAIEVMAQSAQTPDGREGMRAFVEKRPAVYPPTRSPTR"
     gene            complement(962..>2343)
                     /locus_tag="VF516_00300"
     CDS             complement(962..>2343)
                     /locus_tag="VF516_00300"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8106127.1"
                     /translation="GRPTASHNARKLLDARELLDAPLTPSFARSLLHLPRAEILDDYL
                     ATAPAEVRPCIGTPDDPGPPLVLGQLGTRAFEDRIWRTIAGLAEGSFRQKANSDGIAV
                     NRGKTGGPAARAAHIHVAERRDLEALGDHLHAHYRAVIAAHGMTGRAEVVDHVFRWET
                     DFDLDWMDGWARNRDAPCERNLVVMIPGKQRGEAVIMADHYDTAYMEDVYDADRGGDT
                     LRAPALGADDNHSATTALMLAAEQLLPLAAAGKLERDVWLVHLTGEEFPADSLGARAL
                     CQGLVERHLVFTAEDGTPRDVSHVHVAGAFVLDMIGHNTHRELDVFQIAPGEGAAAHR
                     LAWRLQRANQRWNRAVAGWNAAPDRHGKGRAHRMPDGSRPPPPFAHLALHGEVRVEWE
                     PRSALYNTDGQVFSDLGVPVVLIMENYDISRTGYHDTLDTMANIDLDYCAAITAIAIE
                     AIADTACAP"
BASE COUNT          315 a          745 c          954 g          329 t
ORIGIN      
        1 gatcgcgaga agcgtccact tcacggcgat ccctcccttg cgaatcgagt gaaaacaata
       61 cgcacggcga tcgcaggcga gtgacgggcc gacaaacagt gtgtgaacgc tggatgacaa
      121 ccggcgcggc gctaccgggt cggcgaccgg gtcggcgggt agacggcggg gcgcttctcg
      181 acgaaggcgc gcatcccctc gcggccgtca ggagtctgcg cgctctgcgc catgacctcg
      241 atcgcctcgg cgtaggcggc ctcgacgtcg agatcgatct ggcggtagaa ggtgcgcttg
      301 ccgagcgcct tggaggccag gctgccgcgg ctggcggcgc gcgcgagctt gtgggtctcg
      361 gcgaccaggt cggcgcgcgg gacgacgcgg ttgaccaggc cccaggcgag cgcggtgcgc
      421 gcgtcgatcg cgtcgccggt catcgcgagc tcgagggcgc ggcgggcgcc gatcgcgcgg
      481 gcgacggcga ccatcggggt gtggcagaac cagccgccgc ggccgccggg ggtggagaac
      541 gcggcgtcct cgacggcgac cgcgaggtcg cagctcgcga ccagctggca gccggccgcg
      601 gtcgcgaggc cctggacgca ggcgacgacc ggcaccggca gccgcgcgat gccgagcatg
      661 acctcggtgc acagcgagag caggcggcgc atgccgtcga gatcgcgctc gatcatgtcg
      721 ccgaagtcgt ggccggcgga gaacaccggg ccggcgccgg cgatgatgat cgcgcggacg
      781 ctggcgggct cggcggcgcg ctgcagcgcc gcgcccaggt cgcgcagcat cgactcggac
      841 agcgagttcc ggcgctcggg tcggttgagg atgacggtgg cgagatcgcc cccggaggga
      901 ccggaaaatt caacgaggac ctggtccatg gccatggctg tatgtatctg tatgttacca
      961 gctacggggc gcacgcggta tcggcgattg cttcgatcgc gatcgcggtg atggcggcgc
     1021 agtagtcgag gtcgatgttg gccatcgtgt caagggtgtc gtggtagccg gtgcggctga
     1081 tgtcgtagtt ctccatgatc aggacgaccg gcacgccgag gtcggagaac acctggccgt
     1141 cggtgttgta cagcgccgag cgcggctccc actcgacgcg gacctcgcca tggagcgcga
     1201 ggtgggcgaa cgggggcggc ggccgggagc cgtcgggcat gcggtgggcg cggcccttgc
     1261 cgtggcggtc gggcgcggcg ttccagccgg cgacggcgcg gttccagcgc tggttggcgc
     1321 gctggaggcg ccaggcgagg cggtgggcgg cggcgccctc gccgggggcg atctggaaca
     1381 cgtcgagctc gcggtgggtg ttgtggccga tcatgtcgag gacgaacgcg ccggcgacgt
     1441 ggacgtgcga gacgtcgcgc ggggtgccgt cctcggcggt gaacaccagg tggcgctcga
     1501 ccaggccctg gcacagcgcg cgggcgccga gcgagtcggc ggggaactcc tcgccggtga
     1561 ggtggaccag ccagacgtcg cgctcgagct tgccggcggc ggccagcggc aggagctgct
     1621 cggcggcgag catcagcgcg gtggtcgccg agtggttgtc gtcggcgccg agcgccgggg
     1681 cgcgcagcgt gtcgccgccg cgatcggcgt cgtagacgtc ctccatgtac gcggtgtcgt
     1741 agtggtccgc catgatcacc gcctcgccgc gctgcttgcc ggggatcatg acgacgaggt
     1801 tgcgctcgca gggcgcgtcg cggttcctgg cccagccatc catccagtcg agatcgaagt
     1861 cggtctccca gcggaacacg tggtcgacga cctcggcgcg gccggtcatg ccgtgggcgg
     1921 cgatcacggc gcggtagtgg gcgtggaggt ggtcgccgag ggcctcgagg tcgcggcgct
     1981 cggcgacgtg gatgtgggcc gccctggccg ccgggccgcc ggtcttgccg cggttgaccg
     2041 cgatgccgtc ggagttggcc ttctggcgga acgagccctc ggcgaggccg gcgatggtgc
     2101 gccagatccg gtcctcgaat gcgcgcgtgc cgagctggcc gagcacgagc ggcgggcccg
     2161 gatcgtcggg ggtgccgatg cacggccgga cctcggcggg cgcggtcgcg aggtagtcgt
     2221 cgaggatctc ggccctgggc aggtgcagca gcgagcgcgc gaacgacggg gtgagcggcg
     2281 catcgagcag ctcgcgcgcg tcgagcagct tgcgcgcgtt gtgactcgcc gtcgggcggc
     2341 cat
//
LOCUS       DASVOR010000013         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_292362,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000013 DASVOR010000000
VERSION     DASVOR010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292362"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..1940)
                     /locus_tag="VF516_00305"
     CDS             complement(375..1940)
                     /locus_tag="VF516_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106128.1"
                     /translation="MRRLGLLAGAAVAAASSACGTDHAGYVLVTVDARPAVHDAAAIT
                     VTLANAGTMRTDTLMLAGKSFPVTFSVSSPGRSGDLAIAVDASDAGGLVIGHGSAMTT
                     VSASAAKVVLDATDFVVNTDYAGDQFPTDDSQAAGFQLAALPDGTWTTVFRDGCMQNA
                     CNVFARRFDRAGKPVTTQAAASTNAFVVTARPTTDLAEPAIAASPNATLAVWNFSDVG
                     TATTTGVACRPIDAAGRLAADQTSIATETASVVSAAAMASGNFAVTWKTTLPAMNDVD
                     AIHMVVVKPDCTPVGAIVELAKGAVFADFVHRGSVASSADQVLFAWITNGELFTSLMP
                     STGAPSPGMKLIAKTTTPAPPLEVEHARVAALPGGGFLIAVSWTLQTGTGPGRIDLFR
                     VDTKGMLSGSPVQVTDQSDGDPLNAESFGLASRPDGSILLAWHVCNANGDSCVLSGRI
                     LQLTADANPMSWAIPVTDVFAIPTTTGGSDKRASVVGLPDAFAVVWSDSSMKPPDTAG
                     QAVRARLIYPPGS"
BASE COUNT          307 a          701 c          810 g          298 t
ORIGIN      
        1 atcgtccatc ggcacggcac ctagcaaagc tgttctccga agagccggct gcgggagttc
       61 tgcacggccg gatctgtggg ggcccggcga gcccaagtcg ccgggctacc cgacacgttg
      121 agcccaccct cgacgttgga cctcgaccga tcctgtgctg cgagtctcga cggcgcgcga
      181 caacagggcg aggtcgacgg tggggtcaga cgtccacgta tcgcttcgct gccgtcaaac
      241 tcaacgctgg agtcaaggtc gacgtcatcg tcgatgtcga cggcgagcgc cacgggaacc
      301 acccgaaggc gcaacttgtg atgaatcatc agagagtcgg ctagggacga cccgggggcc
      361 cacgcttcct gcgtctagct gccggggggg tagatgaggc gggcgcgcac cgcctggccg
      421 gcggtgtcgg gcggcttcat gctcgagtcg gaccacacga cggcgaaggc gtcgggcagg
      481 ccgaccaccg aggcgcgctt gtcgctgccg ccggtggtgg tcgggatcgc gaacacgtcg
      541 gtgaccggga tcgcccagga catcgggttc gcgtcggcgg tgagctgcag gatccggcca
      601 gacagcacgc agctgtcgcc attggcgttg cagacgtgcc aggcgagcag gatgctgccg
      661 tcggggcggc tggcgaggcc gaacgactca gcgttgagcg ggtcgccgtc gctctgatcg
      721 gtgacctgca ccggcgagcc ggacagcatg cccttggtgt cgacgcggaa caggtcgatc
      781 cggccggggc cggtcccggt ctgcagggtc cagctgacgg cgatcaggaa gccgccgccg
      841 ggcagcgcgg cgacccgtgc gtgctcgacc tcgaggggag gagcgggggt ggtggtcttc
      901 gcgatcaact tcatgccggg cgagggagcg ccggtgctcg gcatcagcga ggtgaagagc
      961 tcgccgttgg tgatccaggc gaacagcacc tgatccgcgc tgctggcgac cgagccgcgg
     1021 tgcacgaagt ccgcgaacac ggcgcccttg gccagctcga cgatggcccc gaccggggtg
     1081 cagtccggct tgaccacgac catgtggatc gcgtcgacgt cgttcatcgc gggcagcgtg
     1141 gtcttccagg tcacggcgaa gttgccgctc gccatcgcgg ccgccgacac gaccgatgcg
     1201 gtctcggtcg cgatcgacgt ctgatccgcg gcgagccggc cggcggcgtc gatcggccgg
     1261 cacgcaacgc cggtcgtggt cgcggtcccc acgtcggaga agttccacac cgcgagggtc
     1321 gcgttcgggc tcgccgcgat cgccggctcc gcgaggtcgg tcgtcggccg ggcggtcacg
     1381 acgaacgcgt tggtgctggc cgcggcctgg gtcgtcaccg gcttgccggc gcggtcgaac
     1441 cggcgcgcga acacgttgca ggcgttctgc atgcagccgt cgcggaacac cgtggtccac
     1501 gtgccgtcgg gcagcgcggc gagctggaac cccgcggcct gggagtcgtc ggtcgggaac
     1561 tgatcgccgg cgtagtcggt gttgaccacg aagtcggtcg cgtcgagcac gaccttcgcc
     1621 gccgaggccg agaccgtggt catcgcgctg ccgtggccga tgaccagacc gccggcgtcg
     1681 ctggcgtcga ccgcgatcgc gaggtctccc gagcggcccg gcgacgagac gctgaacgtg
     1741 accgggaacg acttgccggc gagcatcagg gtgtcggtcc gcatcgtgcc ggcgttggcg
     1801 agcgtcacgg tgatcgccgc ggcgtcgtgc accgccggtc gggcgtcgac cgtgacgagg
     1861 acgtagccgg cgtggtcggt gccgcacgcg ctggaggcag cagcgaccgc ggcgccggcg
     1921 aggagaccga ggcgccgcat cacttcccga acacgatgtt gcccagcgag gtcgacggcg
     1981 gaccggctcc gggccgggtg agctcgtagg ccgccccgcc ggcgccggcg gcgatcacgc
     2041 cgacgccggt ccagaaccac caccgcgtgt agagcggatg gtcggccggc ggcggcggtg
     2101 cggccggggc gggggc
//
LOCUS       DASVOR010000014         7938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_233882,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000014 DASVOR010000000
VERSION     DASVOR010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7938
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_233882"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VF516_00310"
     CDS             <1..1348
                     /locus_tag="VF516_00310"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8106129.1"
                     /translation="PARPAAPGGGDTARAAPDAAAQSLHVPIRSMTLPNGLRVVLSRD
                     TTAPVVALAVYYNIGFRIEPRDRTGFAHLFEHMMFQGSQNLGKMEFIKMVQSNGGVLN
                     GSTRFDFTNYFEVVPANTLETMLWGEADRMRGLAVTQDNLTNQQGVVKSEVRVNVLNR
                     PYGGFPWLDMPQYANKNWHNAHNFYGDLTDLDHATLADVQTFFHQYYTPNNAALVVSG
                     DLDYEQTEAWIQKYFAAIPRGPDPVRPDLTEPRQDQEIRAEKVDPLADRPAFAVAYHV
                     PDRNTPEFYAFAILHEVLLDGHDSALFQKLVHDRKLTGELSGGVNLLGNQFDYAGPML
                     WIVNAVHDKDKTVDQIVAAIDEAIEPLRTQPISASTLSRARVKMRAQLYDMIEGFFGF
                     GRADLLASFALFDNDPEKINRLEGELMKVTPEQIQKTAQQYLRPTNRTVLALKAGK"
     gene            1363..2739
                     /locus_tag="VF516_00315"
     CDS             1363..2739
                     /locus_tag="VF516_00315"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8106130.1"
                     /translation="MRHMPIALSIAAALAALPARADKQTPPPPAAPRPFPLPSHQDSA
                     FDNGLKVTTVAYGSVAKAAVVLMIRGGNAEEDASQTWLANLMGRSLPEGTRSQTAAQL
                     AEAAAAMGGDLEVGVEADQTTITLEVLSEFAPRAIALVAEVARNPAFPPADVARVQTD
                     LVRQLAVLRTQPGQLAREQFSAALYPKHAYGRTMPTEALLKSYTVDQVRAFHAAHVGA
                     ARSHLYVAGRFDDAAVQKAARDALADWARGPEHAPVKVAPTVRAEVRVDDRPGAVQST
                     LIVGLRVVTPSSADYVPLLVTDALLGGSFGSRITSNIREQKGYTYSPFSAVQTPEGNA
                     VWSEQADVTTNVTGASLTEIFKEVERLRAEPPTAEELRGIQSYLAGVFVLRNSTRQGI
                     VRQLSFVEKHGLTEDYLRNFAHRVWAVTPADVRRIASTYLDPKKMTIVVVGDRTQIDE
                     QVAPWAGW"
     gene            complement(2819..4402)
                     /gene="pckA"
                     /locus_tag="VF516_00320"
     CDS             complement(2819..4402)
                     /gene="pckA"
                     /locus_tag="VF516_00320"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEX8106131.1"
                     /translation="MAFSLDKQGISIAEIHRNASPAFLYEAALKFEKGSTISSAGALI
                     AYSGKKTGRSPTDKRVVDEPEVRDDVWWGNVNIKLDPHSFLVNRERAVDYLNTRDRLY
                     VIDGYAGWDPRHQLKIRVICARAYHALFMHNMLIRPTADQLASFGEPDYVIYNAGGFP
                     ANRHTAGMTSTTSVDLSFERREFVILGTEYAGEMKKGVFTIMNYLMPKNGILSMHCSA
                     TQARDGSDTSVLFGLSGTGKTTLSADPKRLLIGDDEHCWSDQGIFNIEGGCYAKVIKL
                     SKEQEPDIWNSLQFGAVLENVVYEPTSHLVDYDNTSITENTRGSYILDTIPNARLPAV
                     GGHPRNVIFLTADAFGVLPPVSRLTPGQAMYHYISGYTAKVAGTEVGVKDPEPTFSPC
                     FGGPFLVWHPTKYAEMLADKLKKHGAQTWLVNTGWSGGPYGVGARIKLKYTRAIIDAI
                     HSGALLSVPTVTDPVFGLAMPTSCPDVPAEILQPRNTWADKAAYDEKAKKVASLFRDN
                     FKKYEAQASAEVRAGGPKL"
     gene            complement(4615..5763)
                     /locus_tag="VF516_00325"
     CDS             complement(4615..5763)
                     /locus_tag="VF516_00325"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX8106132.1"
                     /translation="MTRISLGLGIVALVLTIRDTGLVQIGTYFQRIGWGWVAVVILEI
                     AITTLDASAIRAFLSPDQDKVGLGSALLSQLAGRAVNAVTPSGNLGEAVKVSVLVEHV
                     SQSRAVATILLYNVVSFTIELVTVGIAALVTAMFLPMPAALRWGLLGGGALVLVLGLA
                     IYALVRRGVLVSAARLAARIPVPGLASVRGWLWKVARPSRYLLAPERLARWEPRLRSV
                     DDKMKLVEGARRRDRHLGIALVALSRLTSMTLSFLLLIAMGETLTIGLVASITVGSFF
                     IYMASTLVPMGIGINEGGYNELFRMLGENPARGTALILARRVALILYAAIGLVLVTAS
                     ETVQRARERQATRTAAVSGPIPALAEVGLVATPPVAPAVSVSVSEIAD"
     gene            complement(5831..6355)
                     /locus_tag="VF516_00330"
     CDS             complement(5831..6355)
                     /locus_tag="VF516_00330"
                     /inference="COORDINATES: protein motif:HMM:NF025918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX8106133.1"
                     /translation="MTSDTIELVERVRRRLKEHENGCQIAGPAPEAAISAAEAALGCR
                     FPPSYRSFLRTFGGIAIPPHLGVVHDFVGVAAEPVDAAHPAGGATDVVRRTLQARVER
                     KLADHLVVVGLGAQHQEWFCLDVSKPNSTGEYPVVLFDAKDNALDQQFYEDFGQMLAE
                     VMGFVADNLDQPLD"
     gene            complement(6483..7373)
                     /locus_tag="VF516_00335"
     CDS             complement(6483..7373)
                     /locus_tag="VF516_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX8106134.1"
                     /translation="MERSVKGLLGGGLRPNDPRRFLIEAMIGAMNADGVVDPRETQAV
                     ERQIASHPLFQGLGPAAARTLIDLSNDAIRFAGSAFGRAPAIAKGLPARIHRLAAYGM
                     AAEVAVADEQLVDGELRFLEALRIALRIAPLESEALVEHARTGHLAEYLEDRYRRIKT
                     LIPVAAEVFVLRALGRGMATDDERFRIRDFFAAIPDLAMQPDDLDGVLYQAFRRPRPP
                     GAQVFGELSQVAATLPDPVDRYWMMVYTLVAELPATVPSWRVIPFTGVMQAAFQITDT
                     DMELAVIDALSFPAAMPRPH"
     gene            7451..>7938
                     /locus_tag="VF516_00340"
     CDS             7451..>7938
                     /locus_tag="VF516_00340"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8106135.1"
                     /translation="MGRPLVLVACLVAGATGSAAAGPGQAQGTKTSKVTIETDPPGAK
                     VYFNVKEDGEVCTTPCTVDAPIGETPIIVEAENRRAIIENLVVPRKTARPLRVSFKLK
                     PAYGSLVIEGSDGATIKIDERAQGTAPRRFDEILAGAHHVVLEKDGKKLYDEFVDVEV
                     DT"
BASE COUNT         1263 a         2769 c         2781 g         1125 t
ORIGIN      
        1 ccccgccagg cccgccgcac ccggcggtgg cgacaccgcc cgcgccgcgc cggacgccgc
       61 cgcgcagtcg ctccacgtgc cgatccgctc gatgacgctg cccaacgggc tgcgcgtcgt
      121 gctgtcgcgc gacacgaccg cgccggtggt cgcgctggcg gtctactaca acatcggatt
      181 ccggatcgag ccgcgcgatc gcaccggctt cgcccacctg ttcgagcaca tgatgttcca
      241 gggctcgcag aacctgggca agatggagtt catcaagatg gtgcagtcga acggcggcgt
      301 gctcaacggc tcgacccggt tcgacttcac caactacttc gaggtcgtcc cggccaacac
      361 gctggagacc atgctgtggg gcgaggccga ccggatgcgc gggctggcgg tgacccagga
      421 caacctgacc aaccagcagg gcgtggtcaa gagcgaggtc cgggtcaacg tgctcaaccg
      481 gccgtacggc gggttcccgt ggctcgacat gccgcagtac gcgaacaaga actggcacaa
      541 cgcccacaac ttctacggcg acctcaccga cctcgatcac gccacgctcg ccgacgtcca
      601 gacgttcttc caccagtact acacgcccaa caacgccgcg ctggtggtgt ccggcgacct
      661 cgactacgag cagaccgagg cgtggatcca gaagtacttc gccgccatcc cgcgcggtcc
      721 cgaccccgtc cggcccgacc tgaccgagcc gcgccaggac caggagatcc gcgccgagaa
      781 ggtcgatccg ctcgccgacc ggccggcgtt cgcggtcgcc taccacgtgc cggatcgcaa
      841 cacgcccgag ttctacgcgt tcgcgatact gcacgaggtg ctgctcgacg gccacgacag
      901 cgcgctgttc cagaagctgg tgcacgaccg gaagctgacc ggcgagctct cgggcggcgt
      961 caacctgctc ggcaaccagt tcgactacgc cgggccgatg ctgtggatcg tcaacgcggt
     1021 gcacgacaag gacaagacgg tcgaccagat cgtcgcggcg atcgacgagg cgatcgagcc
     1081 gctgcgcacg cagccgatca gcgcgagcac cctgtcgcgc gcccgggtca agatgcgggc
     1141 gcagctgtac gacatgatcg agggcttctt cgggttcggc cgcgccgatc tgctggcgtc
     1201 gttcgccctg ttcgacaacg acccggagaa gatcaaccgg ctcgagggcg agctcatgaa
     1261 ggtgacgccc gagcagatcc agaagacggc gcagcagtac ctgcggccga ccaaccgcac
     1321 cgtcctcgcg ctcaaggcgg gaaagtagcc gtggagaacc tcatgcgcca catgcccatc
     1381 gcgctgtcga tcgccgccgc gctggcggcg ctgccggccc gcgccgacaa gcagaccccg
     1441 ccgccaccgg cggccccccg gccgttcccg ctgccgtcgc accaggactc cgcgttcgac
     1501 aacggcctga aggtcaccac cgtggcctac ggctcggtcg ccaaggccgc ggtcgtgctg
     1561 atgatccggg gcggcaacgc cgaggaggat gccagccaga cctggctcgc caacctgatg
     1621 ggccgatcgc tgcccgaggg cacgcgcagc cagaccgcgg cgcagctcgc cgaggccgcc
     1681 gcggcgatgg gcggcgacct cgaggtcggc gtggaggccg atcagacgac gatcacgctc
     1741 gaggtgctgt cggagttcgc gccgcgcgcg atcgcgctgg tcgccgaggt ggcgcgcaac
     1801 cctgcgttcc cacccgccga cgttgcgcgg gtccagaccg acctggtgcg ccagctggcg
     1861 gtcctgcgga cccagcccgg gcagctggcg cgcgagcagt tcagcgccgc gctctacccc
     1921 aaacacgcgt acggccggac gatgccgacc gaggcgctgc tcaagagcta caccgtcgac
     1981 caggtgcgcg cgttccacgc cgcccacgtc ggtgccgcgc gctcccacct ctacgtggcc
     2041 ggccggttcg acgacgccgc ggtgcagaag gccgcgcgcg acgcgctcgc cgactgggcg
     2101 cggggccccg agcacgcgcc ggtcaaggtc gcgccgaccg tgcgggccga ggtgcgcgtg
     2161 gacgaccggc cgggcgccgt gcagtcgacg ctgatcgtcg ggctccgggt ggtgacgccg
     2221 agcagcgccg actacgtccc gctgctggtc accgacgccc tgctcggcgg ctcgttcggc
     2281 tcgcgcatca cgtcgaacat ccgcgagcag aagggctaca cctactcgcc gttctcggcg
     2341 gtccagaccc ccgaaggcaa cgcggtgtgg agcgagcagg ccgacgtcac caccaacgtc
     2401 accggcgcgt cgctgaccga gatcttcaag gaggtggagc ggctgcgcgc cgagccgccg
     2461 accgccgagg agctgcgcgg catccagtcg tacctcgccg gcgtgttcgt gctgcgcaac
     2521 tcgacccggc agggcatcgt ccgccagctg agcttcgtcg agaagcacgg gctgaccgag
     2581 gactacctgc gcaacttcgc gcaccgggtg tgggcggtca cgccggccga cgtgcggcgg
     2641 atcgcgtcga cctacctcga tccgaagaag atgacgatcg tcgtggtcgg cgaccgcacg
     2701 cagatcgacg aacaggtggc gccgtgggcc ggttggtgaa cgtgtaggct gaaccccgac
     2761 gggaagaaca cgccatgcca gcggcgcgcc cggtcacaag tgacgcgccg gacgggggct
     2821 acagcttcgg gccgccggcg cggacctcgg cggacgcctg cgcctcgtac ttcttgaagt
     2881 tgtcgcggaa cagcgaggcg accttcttgg ccttctcgtc gtaggcggcc ttgtcggccc
     2941 aggtgttgcg cggctgcagg atctcggccg gcacgtcggg gcacgacgtc ggcatcgcga
     3001 ggccgaacac cggatcggtc acggtcggga cgctcagcag cgcgcccgag tggatcgcat
     3061 cgatgatcgc gcgggtgtac ttgagcttga tccgcgcgcc gacgccgtac ggtccgcccg
     3121 accagccggt gttgaccagc caggtctgcg cgccgtgctt cttgagcttg tcggcgagca
     3181 tctcggcgta cttggtcggg tgccagacca ggaacgggcc gccgaagcac ggcgagaacg
     3241 tcggctcggg gtccttcacg ccgacctcgg tgccggcgac cttggcggtg tagcccgaga
     3301 tgtagtggta catcgcctgg cccggcgtca gcctggacac cggcggcagc acgccgaacg
     3361 cgtcggcggt caggaagatg acgttcctgg gatggccacc gaccgcgggg agcctggcgt
     3421 tggggatggt gtcgaggatg tagctgccgc gcgtgttctc ggtgatcgac gtgttgtcgt
     3481 agtcgaccag gtgcgaggtc ggctcgtaca cgacgttctc cagcaccgcg ccgaactgca
     3541 aggagttcca gatgtcgggc tcctgctcct tcgacagctt gatcaccttg gcgtagcagc
     3601 cgccctcgat gttgaagatg ccctgatcgc tccagcagtg ctcgtcgtcg ccgatcagca
     3661 ggcgcttggg atcggccgac agcgtggtct tgccggtgcc cgacagcccg aacaggaccg
     3721 acgtgtcgct gccgtcgcgc gcctgggtcg ccgagcagtg catcgacagg atgccgttct
     3781 tgggcatcaa atagttcatg atcgtgaaca cgcccttctt catctcgccg gcgtactcgg
     3841 tgccgaggat gacgaactcg cggcgctcga acgacaggtc gaccgacgtg gtcgaggtca
     3901 tgccggcggt gtggcggttg gccgggaacc cgccggcgtt gtagatgacg tagtcgggct
     3961 cgccgaagct cgccagctgg tcggcggtgg gccggatcag catgttgtgc atgaacagcg
     4021 cgtggtaggc gcgcgcgcag atcacgcgga tcttgagctg gtgccggggg tcccagccgg
     4081 cgtagccgtc gatcacgtac agccggtcgc gggtgttgag gtagtccacc gcgcgctcgc
     4141 ggttgaccag gaagctgtgg gggtcgagct tgatgttgac gttgccccac cacacgtcgt
     4201 cgcggacctc gggctcgtcg accacgcgct tgtcggtcgg gctgcggccg gtcttcttgc
     4261 cggagtacgc gatcagcgca ccggcggacg agatcgtcga gcccttctcg aacttgagcg
     4321 cggcctcgta gaggaaggcc ggcgacgcgt tgcggtgaat ttctgcgatc gagatccctt
     4381 gcttgtcgag cgagaacgcc atacgcgagg ctccggtcgg aggtagaggg tggaagtcga
     4441 tatataccaa caatcctcgg gtgggcgggc ggccggcgcg gcccgtcgtc gcggcgtggc
     4501 gcgtgcgttc ccgctcgcag cccgcgctca cctggtgtcg agctcacaca acgtccgatg
     4561 acgcacagcg ttccgggcga cccgggcggg cggggacgcc gggtttcccg ccgcttagtc
     4621 cgctatctcg gagacggaca cggacacggc cggggccacc gggggggtcg ccacgaggcc
     4681 gacctcggcg agcgccggga tcgggccgct gaccgcagcc gtacgcgtcg cctggcgctc
     4741 gcgcgcgcgc tgcacggtct cgctcgcggt caccagcacg agcccgatcg cggcgtacag
     4801 gatcagcgcc acgcggcggg ccaggatcag cgccgtgccg cgcgcggggt tctcgccgag
     4861 catgcggaac agctcgttgt agccgccctc gttgatcccg atccccatcg gcaccagggt
     4921 cgacgccatg tagatgaaga agctgcccac ggtgatcgac gcgaccaggc cgatcgtcaa
     4981 cgtctcgccc atcgcgatca gcagcaggaa cgacagcgtc atggacgtca gccgcgacag
     5041 cgcgaccagc gcgatgccga ggtggcgatc gcgccgccgc gcgccctcga ccagcttcat
     5101 cttgtcgtcg acgctgcgca gccgcggctc ccagcgggcg agccgctcgg gggccagcag
     5161 atagcgcgac ggccgcgcga ccttccacag ccagccgcgc acgctggcca ggccggggac
     5221 cgggatccgg gcggcgagcc gggccgcgct gaccagcacg ccgcggcgga ccagcgcgta
     5281 gatcgccagc ccgagcacga gcacgagcgc gccgccgccg agcagccccc agcgcagcgc
     5341 cgccggcatc ggcaggaaca tcgccgtgac cagcgcggcg atcccgacgg tgaccagctc
     5401 gatcgtgaag ctgacgacgt tgtacaagag gatggtcgcg accgcgcgcg actggctgac
     5461 gtgctcgacc aggaccgaga ccttcaccgc ctcgccgagg ttgccgctcg gggtgacggc
     5521 gttgaccgcg cggccggcga gctgcgagag caacgcgctg ccgagcccga ccttgtcctg
     5581 atcgggcgag aggaacgcgc ggatcgcgga ggcgtcgagc gtggtgatcg cgatctcgag
     5641 gatcacgacc gcgacccagc cccagccgat ccgctggaag taggtcccga tctggaccag
     5701 cccggtgtcg cggatcgtga gcacgagcgc aacgatgccg agccccagcg agatgcgggt
     5761 cacccaccta gcccacgtcg gccggctcat ccaggaaacc tatagcagaa gccgggccgg
     5821 gcgttagcgc tcagtcgagc ggctggtcca ggttgtcggc cacgaagccc atgacctcgg
     5881 ccagcatctg cccgaagtcc tcgtagaact gctggtccag ggcgttgtcc ttggcatcga
     5941 acagcacgac cgggtactcg ccggtgctgt tcggcttgga gacgtcgagg cagaaccact
     6001 cctggtgctg cgcgccaagt ccgacgacga cgaggtgatc cgcgagcttg cgctcgacgc
     6061 gggcctgcag cgtgcggcgg actacatcgg ttgcaccgcc ggccgggtgg gccgcgtcga
     6121 ccggctcggc ggcgacgccc acgaagtcat ggaccacccc caggtgaggt gggatcgcga
     6181 tgccaccgaa cgtgcgcagg aagctccggt acgagggcgg gaacctgcag cccagcgcgg
     6241 cctccgccgc cgagatcgcc gcctccggcg cggggccggc gatctggcat ccgttctcgt
     6301 gctccttgag gcggcgacgc acgcgttcca cgagctcgat ggtgtccgac gtcatgacgt
     6361 gtcgtccatt atgcaccgcg gatcgcgccg tgcggtgcgt cgtgcggtga gagccacccg
     6421 gcgtgctgcg cagcgcgtga tccgcgcacg cagccgttga cattctcccg gatccgctgg
     6481 agtcagtgcg gccgcggcat cgcggccggg aagctcagcg catcgatgac ggcgagctcc
     6541 atgtcggtgt cggtgatctg aaacgcggcc tgcatcacgc cggtgaacgg gatcacgcgc
     6601 cagctcggca cggtcgccgg cagctcggcg accagcgtgt agaccatcat ccagtagcgg
     6661 tcgaccggat cgggcaaggt cgccgcgacc tgcgacagct cgccgaacac ctgcgcgccc
     6721 ggcggccgcg gcctgcggaa cgcctggtac agcacgccgt cgaggtcgtc cggttgcatc
     6781 gcgaggtcgg ggatcgccgc gaagaagtcg cggatccgga accgctcgtc gtcggtggcc
     6841 atcccgcggc ccagcgcacg cagcacgaac acctcggccg cgaccgggat cagcgtcttg
     6901 atccggcgat agcgatcctc gaggtactcg gcgaggtggc cggtgcgggc gtgctcgacc
     6961 agcgcctcgg actccagcgg cgcgatccgc agcgcgatcc gcagcgcctc gaggaagcgc
     7021 agctcgccgt cgaccagctg ctcgtcggcc accgcgacct cggcggccat cccgtacgcc
     7081 gcgagccggt ggatccgcgc cggcaggccc ttggcgatcg ccggcgcgcg gccgaacgcg
     7141 ctgccggcga accggatcgc gtcgttggac aggtcgatca gcgtgcgggc cgccgccggg
     7201 ccgaggccct ggaacagcgg atggctggcg atctggcgct cgaccgcctg ggtctcgcga
     7261 gggtcgacga ccccatcggc gttcatcgcg ccgatcatcg cctcgatcag gaaccgccgc
     7321 ggatcgttgg gccgcagccc gccgccgagc agtcccttga cgcttctttc catggggcaa
     7381 gtgtaatgga acgaggcgcc gcgagcatgg aactgggcgc cgcccgggtg cgcgcgttat
     7441 gatccggccg atgggacgac cgctggtcct cgttgcgtgc ctggtggccg gcgccaccgg
     7501 cagcgccgcg gccggcccgg gccaggccca gggcacgaag accagcaagg tcacgatcga
     7561 gaccgacccg ccgggcgcca aggtctactt caacgtcaag gaggacggcg aggtctgcac
     7621 gacgccgtgc accgtcgacg cgccgatcgg cgagaccccg atcatcgtcg aggccgagaa
     7681 ccggcgcgcg atcatcgaga acctggtcgt gccgcgcaag acggcgcggc cgctgcgggt
     7741 gtcgttcaag ctcaagccgg cctacggctc gctcgtcatc gagggcagcg acggcgcgac
     7801 gatcaagatc gacgagcgcg cccagggcac cgcgccgcgc cggttcgacg agatcctggc
     7861 cggcgcccac cacgtcgtgc tcgagaagga cggcaagaag ctctacgacg agttcgtcga
     7921 tgtcgaggtc gacaccga
//
LOCUS       DASVOR010000015         4235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58536,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000015 DASVOR010000000
VERSION     DASVOR010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4235
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58536"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF516_00345"
     CDS             complement(<1..347)
                     /locus_tag="VF516_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEX8106136.1"
                     /translation="MATVSQVLTALVAALHSYFLVLEMFLWTTPTGQRVFGRTEAEQE
                     SSKVLAANQGLYNGFLAAGLVWSLVAGAPVALPVRVFFLACVVVAGVYGGLTATRRIL
                     FVQALPAAAALAVT"
     gene            complement(356..970)
                     /locus_tag="VF516_00350"
     CDS             complement(356..970)
                     /locus_tag="VF516_00350"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8106137.1"
                     /translation="MQPLCQITWATPAGRLRAIEPEPDEVVRYAGALAAAYNDPRNAP
                     LLGHTEDVDEDDVVAHYADVAEAGGHNFLVFLDPTGGSAPRLDGALAADADLRKVGGG
                     AAEFAFMVASPAAQGKGLGTRVALMIHAFGFRRLELERVYASIIPGNAASRRVFEKLG
                     YQVDASPAARAYADEPGDITMAIDRAGFERSHAQAMAEIEIAVR"
     gene            1427..2422
                     /locus_tag="VF516_00355"
     CDS             1427..2422
                     /locus_tag="VF516_00355"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8106138.1"
                     /translation="MVSFERRKTLRESRDVVAVPTRAVAPATGRLVIATIGIDRYRHW
                     PALTNAVEDATGAAALFQRLGFEEVVPPLLDDRATGGAIEALVADELTSLGASDSLIV
                     FYAGHGGTRTQQVGGRAVRTGYLIPVDGSDDARVTSWIEIDPWLRRIAKLPPRHILVI
                     LDACFSGIALSSAVKWGRNSGALIELPFAVANTRPSRLVITSALDDERAMDSGPMPGH
                     SLFTGCLMEALTGGLPPIGTHDGRPMTIGSEIGRYVRNRVQTYPGRPGWQQTPDLGTF
                     DYDERGEMLIPVLIGDEAAIAPTSVARSSAQILRFTRHTAFESCVARIWEGTTCG"
     gene            2419..3831
                     /locus_tag="VF516_00360"
     CDS             2419..3831
                     /locus_tag="VF516_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106139.1"
                     /translation="MIKKTRIAVTLACWMVSACATIQSSTTYSQTLLTTRTSVVTNPN
                     SRSYTSTTEVEGHDLLVTLRSEESCRTSVVAIYRKEAHVTRTPTTGPLGLVMSPTTSL
                     VAGLLLGGLGAYSYLRADSLAMTGESSQSTPADYRTTGLVSGAIGGGLLVASLVDSIR
                     LRDTTGFVGDVEGAPDVRTDPCHAQIFSNRRVGASVATLAWEVAATSDGSGQVRLDLH
                     GLPESAFAAGELQLELHLIDTIIPLKVAAEPTRQLLEALAADTTSRVSSDREARAVTL
                     CEEHVAAASAVSIEADTPADAIAQAETRWSAARATCGARWQSTHEQQFMAYRAKLAAT
                     AAQRARNQCATAGEAAAQLLDRSNESGERVDLDAIARALAERCSDATNAKPVQDSVIA
                     RAHQLQRARAQAEKARTQAENAAADELDESDPSPRQWPLTSGSKVCTKGCPCGNTCIS
                     CTKTCGKAPGTEPRVHRRRR"
BASE COUNT          711 a         1438 c         1466 g          620 t
ORIGIN      
        1 gtcacggcga gcgcggcggc ggccggcagc gcctggacga acaggatccg gcgcgtcgcg
       61 gtcagcccgc cgtagacccc ggcgaccacg acacaggcga ggaagaacac gcggaccggc
      121 agcgcgaccg gcgcgccggc cacgagcgac cacaccagcc ccgcggcgag gaacccgttg
      181 tagagcccct ggttcgcggc gagcaccttg ctcgactcct gctcggcctc ggtccgcccg
      241 aacacgcgct ggccggtcgg cgtggtccac aggaacatct ccaggaccag gaagtacgag
      301 tggagggcgg cgacgagcgc ggtgagcacc tgcgagacag tggccatgcg gccgactatc
      361 gcaccgcgat ctcgatctcg gccatcgcct gggcgtgcga gcgctcgaat cccgcccggt
      421 cgatggccat cgtgatgtcg cccggctcgt cggcgtacgc ccgcgccgcc gggctggcat
      481 cgacctggta gcccagcttc tcgaacaccc ggcgcgacgc ggcgttgccc gggatgatcg
      541 acgcgtagac ccgctcgagc tcgagccggc ggaagccgaa cgcgtggatc atcagcgcga
      601 cccgcgtccc cagccctttg ccctgcgctg ccggcgacgc caccatgaac gcgaactcgg
      661 ccgcgccgcc gccgaccttg cgcaggtcgg catcggcggc cagcgctccg tcgaggcggg
      721 gggcagagcc ccccgtggga tcgaggaaca ccaggaagtt gtggccgccg gcctcggcga
      781 cgtcggcgta gtgggcgacc acgtcgtcct cgtcgacgtc ctcggtgtgg ccgagcagcg
      841 gcgcgttgcg cgggtcgttg tacgcggcgg ccagcgcccc ggcgtaccgc acgacctcgt
      901 cgggctcggg ctcgatcgcc cgcagccgtc cggccggggt cgcccacgtg atctgacaca
      961 gcggctgcac gcgcggcagc gtaccccagc ccggaggatc ggtctcgctg cagccgctcc
     1021 acgcgccgga gccagcaagg ttccatcggc gctttgacca cgtagccatc acgatgaaga
     1081 gccctttcat gctcgatacg cgcacccgcg acccgttgcg gtgaacacga ttgaaggcgg
     1141 cagtcaactc gcccgccgtg gcatcgggca tcgcgcccgg cgcgtgctcg atcgcggcag
     1201 ctgtgatcat cgaggcgtgc cacccgcttg ggtactgtgc tcccgaactc gatccatcga
     1261 agtcctcggt aaggtccacg taacgcgcgg ggctgcaccc cgctgtcggg tgccggcggt
     1321 cgagctgctg gcaggtcgtg atgggtctac ccgtgccgca atcggaccag acctacatcg
     1381 gccacggtca tgtttgctgt cgcgactagc gagatcgtgt cgcccaatgg ttagcttcga
     1441 gaggaggaag accctgcgcg agagtcgcga tgtcgttgca gtccccaccc gcgcggtagc
     1501 gccggcgacg gggcgccttg tcatcgccac gatcggcatc gatcgctacc gtcactggcc
     1561 agcgctgacg aacgcggtcg aagatgcgac gggtgcggcg gcgttgttcc agcggctcgg
     1621 gttcgaggag gtcgtaccgc cgctgctcga tgatcgggcg accggtggcg cgatcgaggc
     1681 gctggtggcc gacgagctga ccagtctggg tgcgagcgac agcctgatcg tgttctacgc
     1741 cggtcatggc gggactcgga cccagcaggt gggtgggcgc gcggtccgca cgggttactt
     1801 gatcccggtc gacggcagcg atgatgcccg ggtcacgtcg tggatcgaga tcgacccgtg
     1861 gttgcgccgg atcgcgaagc tgccgcccag gcacatcctg gtgatcctcg acgcgtgctt
     1921 cagcggtatc gcgctgtcat cggcagtcaa gtggggacgt aatagcggcg ccctgatcga
     1981 gctgccgttc gcggtcgcga acacgcggcc gagccggctg gtcatcacgt cggcgctcga
     2041 cgatgagcga gcgatggact cggggccgat gcccggtcac tcgctgttca ccggctgcct
     2101 gatggaagca ttgactggcg gcctgccacc gatcgggacg cacgatggcc ggcccatgac
     2161 gatcggctcg gagatcggac gctacgtgcg gaatcgtgtg cagacgtatc cggggcgccc
     2221 cggctggcag cagacaccag atctcggcac gttcgactac gacgagcgag gcgagatgct
     2281 gatccccgtg ctgattggag acgaggcggc gattgcgccg acgagtgtgg cgagaagttc
     2341 cgcccagatc ttgcgcttca cgcgccacac tgccttcgag tcttgtgtag caaggatatg
     2401 ggagggaacg acatgtggat gatcaagaaa acgcgaatcg cagtgacact tgcatgctgg
     2461 atggtgtcgg cgtgcgccac catccaatca tcgacgacat attcgcagac tctgctgacg
     2521 acgcgaacct cggtggtcac gaacccgaac tccaggtcgt acacatctac cacggaggtg
     2581 gagggacacg acctgctggt aaccctccgc agtgaggaat cgtgcaggac cagcgtggtc
     2641 gcgatctacc gcaaggaggc gcacgtcacg cggaccccga cgacgggtcc gctcggcctg
     2701 gtgatgtcac cgacgacgtc actggtcgcc ggtctgctgc tcggtggcct cggagcctac
     2761 agctatctcc gtgccgacag cctcgccatg accggggaat cgagccagag cacgccggcg
     2821 gactatcgaa ctaccgggct ggtgagcggt gcgatcggcg gcgggttgct cgtcgcctcg
     2881 ctcgtcgact ccatccggtt gcgcgacacg accgggttcg tcggagacgt cgaaggtgca
     2941 ccggacgtcc ggaccgatcc atgtcatgcg cagatcttct cgaacaggcg cgtcggcgcg
     3001 agtgtagcca cgctcgcgtg ggaggtggcc gccacgagcg acggcagcgg ccaggtccgc
     3061 ctcgacctcc acggcctgcc agagagcgcg ttcgccgcgg gggagctgca gctcgagctg
     3121 cacctgattg acacaatcat tcccttgaag gtcgccgcgg agccgactcg tcaactactc
     3181 gaagcgctcg cggccgacac cacgtcgcgg gtctcgagcg accgcgaggc gcgcgcggtc
     3241 acgctctgcg aggagcacgt cgccgccgcc tcggcggtgt cgatcgaggc ggatacgccg
     3301 gccgacgcca tcgcgcaggc cgaaacgaga tggagcgcgg ctcgcgcaac ctgcggcgcg
     3361 cgatggcagt cgactcacga acagcagttc atggcatatc gcgcgaagct cgccgcaacc
     3421 gctgcccagc gagcccggaa ccagtgtgcc accgcgggcg aagcggccgc acagcttctg
     3481 gatcgcagca acgaatccgg cgagcgtgtg gatctggacg ccattgccag ggcgctcgcc
     3541 gagcgatgca gtgacgcgac gaacgcgaag ccggtccagg acagcgtcat cgcgcgagcg
     3601 catcagctgc agcgagctcg agcgcaggcg gagaaggcac gaacccaggc cgagaacgcg
     3661 gctgccgacg agcttgacga gtccgacccg tcgccacggc aatggccgct cacgtcgggg
     3721 agcaaggtct gcacgaaagg ctgtccctgc ggcaacacat gcatctcgtg cacgaagacc
     3781 tgcggcaagg ctcccgggac cgaaccgcgc gtgcacagac gaaggcgctg acgacctgcg
     3841 ggcgaatgtt gccaacggtc tcgagtgcgc cgccgccgac ggacctctac tgcgcccaaa
     3901 cgcgaatcgt tgagatcgca gcgagccaag tcagccggaa ggctctccgg gcgggggagg
     3961 tctgcgcttg ccacaacatc gtcagctagg ccacgagatg ccagcgtcgc gacggtcaca
     4021 gtcgacggcg tggtgaatct tccgggctaa ggttaccggc catgcagccc gcgcgtcttc
     4081 gcccgctcgc cgcgattgcc atgacggtcg ttgtcgctgc gtgcggtcgc tccggcgcgc
     4141 ccaggccggc cgacgatcac cgcccgggcg gctccagcag cctcggcgac ggcgtccacg
     4201 ccgagccagc cccgagcccg gccggcagcg acgcg
//
LOCUS       DASVOR010000016         5647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_263157,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000016 DASVOR010000000
VERSION     DASVOR010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5647
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263157"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF516_00365"
     CDS             complement(<1..237)
                     /locus_tag="VF516_00365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106140.1"
                     /translation="MQRRHSRRHLFWRGGVAAVSNALMFTGLYPLLPHAWRALSGHDL
                     GDRPVRTALVEWGASAALSAARPLGFLPLPGARTH"
     gene            complement(241..612)
                     /locus_tag="VF516_00370"
     CDS             complement(241..612)
                     /locus_tag="VF516_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106141.1"
                     /translation="MAESQILSVIRMWAAVAWADGVMSGTEAESLGRLIRHADLTDDE
                     RAGAREFLATRTELPETYLTNLTPDARRGVYRAACRMAVVDHEFSVTERALLDRLRNL
                     LGIAVDIAQQIEAEVPGLTVR"
     gene            complement(621..938)
                     /gene="cyaY"
                     /locus_tag="VF516_00375"
     CDS             complement(621..938)
                     /gene="cyaY"
                     /locus_tag="VF516_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR03421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEX8106142.1"
                     /translation="MDEASFDRVARDELHQLENAFSDIDPDEVEVSSSDGVLRLDLRD
                     GTRIVINSHRAARQIWMAAIASAWHFDPTGDGVWRASRTGEELRPTLARLVRERTGLT
                     VPL"
     gene            977..2437
                     /locus_tag="VF516_00380"
     CDS             977..2437
                     /locus_tag="VF516_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106143.1"
                     /translation="MAGRPPDGDPEPDGHGAPALRDGRVTLLPSVTRSALSTRIPGNA
                     PLLDLSGFQDVSETPVPARSDDRPRGARAGDVIGNRYIVEGQLGRGGMGRVMRVRHSA
                     LGKVFALKLIRSSIATNPKIREMFYREARLASALTHDNICSIVDFGQDEVFGLFMVME
                     LLEGQTVHGKLKHGRLAPRVACDILWQVADAVRFIHSRAIVHGDIKTENIFLVRTTVQ
                     RRLVKLLDFGLARPDLGRREGIDGTPEYLAPERQDGAPASQASDIYALGIVFFELLTG
                     RVPFSGAMAEVLLHHKVTEVPAPSAQLSEPLDERADALVRRATAKDPANRHPDVAALL
                     YELRALMGMLGMDQSRRRTIASEAARERRDLDHRIKAAAELFGAAPIPMASCDPDGRV
                     RTANPAFLEFLGVAGDAVDLQLRDSALPELYPELFADLEQVATQRRQVKRIIYLHEGG
                     DRVVEAAVLMSAAPTAAEVTAGQVHLVLHPLRTRSA"
     gene            complement(2502..2762)
                     /locus_tag="VF516_00385"
     CDS             complement(2502..2762)
                     /locus_tag="VF516_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106144.1"
                     /translation="MTARLRSLAVAARAKRMAQRSQRPGGMNRTSTLSVVLLVSTIFT
                     ASMLHARAARPAVEPSAEMSAGQGDDGAEQGYWQIWAAATAQ"
     gene            complement(2984..4372)
                     /locus_tag="VF516_00390"
     CDS             complement(2984..4372)
                     /locus_tag="VF516_00390"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8106145.1"
                     /translation="MRMCCHVAVILGVIGCLAAPVHADPRSDIVAKTRQAMASYDAMD
                     YEAARRLLNQALALAKRAKLDRDPVVARVYLDLGIAQLAASDPEAAKVAFLSAVQIDP
                     RITIDPAYKSAELVRMLDDAKAAAGDAGEPAGDDCRSVRGIQHAPVEGARRGVAQPIE
                     VLIGGDVSPARVIVMYRPEGAIDFTEIRLTRQSGCRYSGAIPAAAMHGALVHYYIAAY
                     DANNKVLTAKGSSGSPNVLELGGGELRHDAEDPAGGAAASPLERSNRGAGPGVVEHAT
                     PAAHGSRVAISASLGAGLGYVSGKTEGENVVQQCCLGTNPFVTSVELRYYLSPRLSLG
                     AVGRLGFPFGANIMGHSTIAPAGLLRLSYALSDRGAGFHISGEAGFGILRNTITLATQ
                     DPTMNTDIVAQGPLLFGSGIGYTFRLSDSFAFLLDLDAIAGIAVVDKLGTAIHLHTSV
                     SADMCAGLAVGF"
     gene            complement(4462..>5647)
                     /locus_tag="VF516_00395"
     CDS             complement(4462..>5647)
                     /locus_tag="VF516_00395"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8106146.1"
                     /translation="GTGRAGPRPPGAGQGPPRAGQGPAVIRALLAVALDVSLGVFSLG
                     GFGLGVFGLGAGCAGAAVRSQTAATDGLIATARANGAQRCAPAELAKAEAHNEFAQHA
                     LDEGNYHDASHEANVAETNAQLAIDRSPRARCVARDAPLPGPGDMDGDGIPDNVDQCP
                     RVPEDLDGFQDADGCPDDDNDGDGITDKLDKCPNDPEDRDGFQDADGCPDPDNDKDGI
                     PDSIDKCPNEPEDFDGFQDADGCPDPDNDGDGVPDVADKCPNEPGVPPDGCPKKYNLV
                     VVTENKIEIKQTVYFDTSRATIKRVSFPLLDEVAQALADHPAIQVEIQGHTDSQGSAR
                     GNLKLSQKRAESVRAYLIKKSIAADRMVARGYGKEVPIADNRTASGRSQNRRVEFVIT
                     SR"
BASE COUNT          766 a         2032 c         2029 g          820 t
ORIGIN      
        1 gtgcgtccgc gcccccggca gcggcaggaa gccgagcggc cgcgccgccg acagcgccgc
       61 cgacgccccc cactcgacga gcgccgtccg caccggccgg tcgccgagat cgtggcccga
      121 cagcgcgcgc caggcgtgcg gcaagagcgg gtacagcccg gtgaacatca gcgcgttcga
      181 caccgcggcg acaccgcccc gccagaacag atggcgtctc gaatgccggc gctgcatgga
      241 ctagcggacg gtcaggccgg ggacctcggc ctcgatctgc tgcgcgatgt cgaccgcgat
      301 gccgagcagg ttgcgcagcc ggtcgagcag cgcgcgctcg gtgaccgaga actcgtggtc
      361 gacgaccgcc atccggcacg cggcccggta cacaccgcgc cgcgcgtcgg gagtcaggtt
      421 ggtgaggtag gtctccggga gctcggtccg ggtcgcgagg aactcccgcg cgccggcgcg
      481 ctcgtcgtcg gtgaggtcgg cgtggcggat cagccgaccc aggctctccg cctcggtccc
      541 ggacatcacg ccgtcggccc acgcgaccgc agcccacatc cggatcacgc tgaggatctg
      601 cgattcggcc atgcgcaatc ctacaacggt acggtgagcc cggtccgctc gcggaccagc
      661 cgagcgagcg tcggccgcag ctcctcgccg gtgcgcgacg cgcgccacac gccgtcgccg
      721 gtgggatcga agtgccaggc ggacgcgatc gcggccatcc agatctggcg cgcggcgcgg
      781 tgcgagttga tcacgatgcg cgtgccatcg cggagatcca gccgcagcac gccatcgctg
      841 ctggacacct cgacctcatc ggggtcgatg tcggagaacg cgttctcgag ctggtgcagc
      901 tcgtcgcgcg cgacgcgatc gaagctggct tcatccatag cggcctcata gcacggtttt
      961 gccgtaccat cgacggatgg cggggcgacc gcccgatggc gatcccgagc cggacggaca
     1021 cggcgcgccg gcgctgcgcg acggccgcgt gacgctgttg ccgtcggtca cgcgctcggc
     1081 gctgtcgacc cggatcccgg ggaacgcgcc cctgctggac ctgagcgggt ttcaggacgt
     1141 ctcggagacc ccggtgccgg cgaggtccga cgaccggccg cgcggcgccc gcgccggcga
     1201 cgtgatcggc aaccgctaca tcgtcgaggg ccagctcggc cgcggcggca tgggccgcgt
     1261 gatgcgggtc cgccactcgg cgctcggcaa ggtgttcgcg ctcaagctga tccggagctc
     1321 gatcgcgacc aacccgaaga tccgcgagat gttctaccgc gaggcccggc tggcctcggc
     1381 gctgacccac gacaacatct gctcgatcgt cgacttcggc caggacgagg tgttcggcct
     1441 gttcatggtg atggagctgc tcgagggcca gaccgtgcac ggcaagctca agcacggccg
     1501 gctggcgccc cgggtcgcgt gcgacatcct gtggcaggtc gccgacgccg tgcggttcat
     1561 ccacagccgc gcgatcgtcc acggcgacat caagaccgag aacatcttcc tggtgaggac
     1621 caccgtgcag cgccggctgg tcaagctgct cgacttcggg ctcgcccggc ccgacctcgg
     1681 ccgccgcgag ggcatcgacg gcacgcccga gtacctcgcc cccgagcgcc aggacggcgc
     1741 gccggcgagc caggccagcg acatctacgc cctcggcatc gtgttcttcg agctgctcac
     1801 cggccgcgtg ccgttctccg gcgcgatggc cgaggtcctg ctccaccaca aggtgaccga
     1861 ggtgccggcg ccgtccgcgc agctgagcga gccgctcgac gagcgcgccg acgcgctggt
     1921 ccgccgcgcg accgccaagg atccggcgaa ccgccacccc gacgtcgcgg cgctgctcta
     1981 cgagctccgg gcgctgatgg gcatgctggg catggatcag agccggcgcc gcacgatcgc
     2041 ctccgaggcc gctcgcgagc gccgcgatct cgaccaccgg atcaaggccg ccgccgagct
     2101 gttcggcgcc gccccgatcc cgatggcgtc ctgcgatccc gacggccggg tccgcaccgc
     2161 caacccggcg ttcctcgagt tcctcggcgt ggcgggcgac gcggtcgatc tccagctccg
     2221 cgactccgcc ctgccggagc tctatcccga gctgttcgcc gacctcgagc aggtcgcgac
     2281 ccagcgccgg caggtcaagc ggatcatcta cctccacgaa ggtggcgacc gggtcgtcga
     2341 ggccgccgtg ctgatgtcgg ccgcgccgac cgccgccgag gtcaccgccg gccaggtcca
     2401 cctcgtgctg cacccgctgc gcactcgcag cgcctagccg gcgagtcttc gagccggcga
     2461 tcgattgtcc ggatgtcccg aggtggcccc ggccgtgcgg ttcactgggc cgttgccgcg
     2521 gcccagatct gccagtaacc ctgctccgcg ccgtcgtcgc cctgccctgc cgacatctcc
     2581 gccgacggct cgaccgccgg ccgcgccgca cgcgcgtgca gcatgctcgc ggtgaagatt
     2641 gtcgagacga gcaagacaac cgagagggtc gacgtgcggt tcatgccgcc cgggcgttgc
     2701 gaccgctgtg ccatgcgctt cgcgcgggca gcgacggcca gcgagcgcag gcgcgcggtc
     2761 acgagatggc cggacgcgat tcgccgaacc gcgtttccgt cgagggtccg gccaactcct
     2821 cgacggcaca cgcgagattt cggcgcaccg cgcggcgccg cagcgcgcgg cagatcccgt
     2881 cgctgcgatc ggggcatttc gcccggccag ccgaggcgcc gccaggtcgc ctaccctgga
     2941 caccacaggt tccaccctgg acggtccgcg gacccacgcg ggttcagaac ccgacggcca
     3001 ggcccgcgca catgtccgcg ctcacgctgg tgtgcaggtg gatcgccgtg ccgagcttgt
     3061 cgacgaccgc gatcccggcg atggcatcca gatcgagcag gaacgcgaag ctgtcgctga
     3121 gccggaacgt gtagccgatt ccgctaccga acagcagcgg accctgggcg acgatgtcgg
     3181 tgttcatggt cggatcctgc gtggcgagcg tgatcgtgtt gcgcaggatg ccgaaccctg
     3241 cctcgccgga gatgtggaag ccggcgccgc ggtcggacag cgcgtagctc agccgcaaca
     3301 ggccggccgg cgcgatcgtg gagtggccca tgatgttggc gccgaacggg aagccgagcc
     3361 ggccgaccgc gccgagcgac agccgggggc tcaggtagta gcgcagctcg acggacgtca
     3421 cgaacgggtt cgtgccgagg cagcactgct gcacgacgtt ctcgccctcg gtcttgcccg
     3481 acacgtagcc gaggccggcg ccgagcgacg ccgagatcgc gacccgggag ccatgcgcgg
     3541 ccggggtcgc gtgctcgacc acgccggggc cggcgccgcg gttcgagcgc tccagcgggc
     3601 tcgcggccgc gccgccggcc gggtcctcgg cgtcgtgacg gagctcgccg ccgccgagct
     3661 cgagcacgtt gggcgagccc gacgagccct tggcggtcaa caccttgttg ttcgcgtcgt
     3721 acgccgcgat gtagtagtgg accagcgcgc cgtgcatcgc cgccgccggg atcgcgccgc
     3781 tgtagcggca gccggactgc cgtgtcagcc ggatctcggt gaagtcgatc gcgccctcgg
     3841 gccggtacat cacgatgacg cgcgccggcg acacatcgcc gccgatcagc acctcgatcg
     3901 gctgggcgac gccgcgccgg gcgccctcca ccggagcgtg ctggatgccg cggaccgagc
     3961 ggcagtcgtc gcccgccggc tcgccggcgt cgcccgcggc cgccttggcg tcgtcgagca
     4021 tccgcaccag ctcggcggac ttgtacgccg gatcgatggt gatcctgggg tcgatctgca
     4081 cggcggacag gaacgcgacc ttggcggcct ccggatccga ggccgcgagc tgggcgatac
     4141 cgaggtcgag gtagacccgg gcgaccaccg gatcgcggtc gagcttggcg cgcttggcga
     4201 gcgccagcgc ctggttgagc agccgccgcg ccgcctcgta gtccatcgcg tcgtagctcg
     4261 ccatcgcctg ccgggtcttg gcgacgatgt ccgaccgcgg atcggcgtgg accggcgccg
     4321 cgaggcagcc gatcacgccg aggatcacgg cgacgtggca gcacatgcgc atctggaacg
     4381 ttctatacca gagcccgccg ggcgtgcggc aagcgtttcg cggtacggac aactgctcgg
     4441 aagcccgacg caaatcccgg cctaccgcga ggtgatcacg aactcgacgc ggcggttctg
     4501 cgaccggccc gaggcggtgc ggttgtcggc gatcggcacc tccttgccgt agcccctggc
     4561 gaccatgcgg tcggcggcga tgctcttctt gatgaggtag gcgcgcaccg actctgcgcg
     4621 cttctgcgac agcttgaggt ttccccgcgc gctgccctgg ctgtcggtgt ggccctggat
     4681 ctcgacctgg atcgccgggt gatcggcgag cgcctgcgcc acctcgtcga gcagcgggaa
     4741 cgacacccgc ttgatcgtcg cgcggctggt gtcgaagtac acggtctgct tgatctcgat
     4801 cttgttctcg gtgacgacga ccaggttgta cttcttcggg cagccgtcgg gcggcacacc
     4861 gggctcgttg gggcacttgt cggcgacgtc cgggacgccg tcgccgtcgt tgtcgggatc
     4921 ggggcagccg tcggcgtcct ggaatccgtc gaagtcctcg ggctcgttgg ggcacttgtc
     4981 gatgctgtcg gggatgccgt ccttgtcgtt gtcgggatcg ggacagccgt cggcgtcctg
     5041 gaacccgtcg cggtcctcgg ggtcgttggg gcacttgtcg agcttgtcgg tgatcccgtc
     5101 gccgtcgttg tcgtcgtcgg ggcagccgtc tgcgtcctgg aagccatcga ggtcctcggg
     5161 gacccgcggg cactggtcga cgttgtcggg gatgccgtcg ccgtccatgt cgccgggacc
     5221 gggcagcggc gcgtccctgg cgacgcaccg ggcgcgcggc gagcggtcga tcgcgagctg
     5281 ggcgttggtc tcggcgacgt tggcctcgtg gctggcgtcg tggtagttgc cctcgtcgag
     5341 cgcgtgctgg gcgaactcgt tgtgggcctc ggccttcgcg agctcggccg gggcgcagcg
     5401 ctgcgcgccg ttggcccgcg cggtcgcgat caggccgtcg gtcgcggcgg tctggctgcg
     5461 cacggcggcg ccggcacagc ccgccccgag gccgaaaacc ccgaggccga aacccccgag
     5521 gctgaaaacc ccgaggctga catcgagcgc caccgcgagc agcgcacgga tcacggccgg
     5581 tccttggccg gcacgaggcg gtccttggcc ggcgccggga ggtcgcgggc cggcgcgacc
     5641 ggtgcct
//
LOCUS       DASVOR010000017         3637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58590,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000017 DASVOR010000000
VERSION     DASVOR010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58590"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /locus_tag="VF516_00400"
     CDS             complement(<1..252)
                     /locus_tag="VF516_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX8106147.1"
                     /translation="MASSEMTEHVAQLYRAIYAVVRRIPAGHVATYGKVAELAGIPGG
                     GRVAGAAMKVSSRAERLPWQRVIGKAGPTRGRIAIHDPVG"
     gene            278..949
                     /locus_tag="VF516_00405"
     CDS             278..949
                     /locus_tag="VF516_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEX8106148.1"
                     /translation="MKIRALYFAVFREQIGRDEDVVELVAGARVRDAIAALAARHAAI
                     GKLRGRFRVAVNQEFSDDDRALADGDELALIPPVAGGGDPPARHVQLLATPLSLDRCI
                     AAVAGATLGGIVTFTGMVRRDSHGAVIDHLEYEAYGAMAVQEMTRLCDEIEAEIPGVR
                     LAVEHRVGRLEVGEVAVVIAAAAPHRAEAFTACRAMIDRLKQRVPIWKKEVGEDGAEW
                     VGLGP"
     gene            993..3620
                     /locus_tag="VF516_00410"
     CDS             993..3620
                     /locus_tag="VF516_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106149.1"
                     /translation="MRMIWHGRGGFRHPHRALIAALASLTVLTAFSRAPARAAPALVV
                     NEPSPGQPAGQYALQVQEDGTAVLHLQWAAADAKPHAIDLALTAFTATGKPAIIVGFD
                     AGKPAALPYLQGVEVPADGAAFRLIASGLEPGVSYGGSLTAIVDRQPTTWNLALIRPG
                     TLPLFRCPDAAQTVRPDDSIVLDVLRATPSGALTVGLRLSPFTTPDSERGDIGFPSGA
                     GGAQLVDELRGVQVAGGHLTVALRTRGLTEGVAYSGKLTLASGGGDTQSCPLGIVLPK
                     LPRGDLVADRPSFTATVTAPWSGTADTSLSLRLFEKTRARPLHGITVALDGTAESPHG
                     GFDLDRNTTFQINGGAAARLTRLPPEDLRDPARAIPPGGQMEVRLALHDLEVGKYAFA
                     LRFNAVNATGPTQRIEITVNVRHGWPWAVLAVVAALLLSFVLTKSIVGWRKRLALRLR
                     IDQLRDQSFVEHSDLAIAVFLRTVLDQTEELLERHPLLPPPDSIDEYITRAERVARIL
                     ICYSSVVDTLRATRCAERIKHYYRDAIADAIHRVGPASLDQATADAIVDQLTATEKAL
                     AEPFPSYWTNITDRGRLLAQQLHDARQTLRALAQIDKLVDTLGHPEQLTAPRPDDLRV
                     AAYDRLYWFGRLLYSRRDDQTHVDQLVQHCKPCQGHDDFPDVDLDGLFRDADRRAWER
                     LKAEIGGAQIEQVQPGQGPEALHPLRFLLRFENRGLASSYFVNNLLAYEWQFEFTPDK
                     PVRRMPRIDSPVRVNQPRVTMYVPAAGKLSVRARIFWPSASPSEPPLELESKQFLVGP
                     NEDLRLLGHLEASTVVHFGMMAAVAIATGLPALYFDNPTFGSYADYVKILAWGIGIDQ
                     GKNLIQLVRSVPPDAPPAT"
BASE COUNT          501 a         1337 c         1313 g          486 t
ORIGIN      
        1 gccgaccgga tcgtggatcg cgatccggcc gcgggtgggg ccggccttgc cgatcacgcg
       61 ctgccacggc agccgctcgg cgcgcgagga gaccttcatg gcggcgccgg cgacgcgacc
      121 gccgccgggg atgccggcga gctcggcgac cttgccgtag gtcgcgacgt ggccggccgg
      181 gatccggcgc accacggcgt agatcgcccg gtagagctgc gcgacatgct cggtcatctc
      241 gctggacgcc atcgccgggc aacgctacca ccggagcatg aagatccgcg cgctgtactt
      301 cgccgtgttc cgggaacaga tcggacgcga cgaggacgtc gtcgagctcg tcgccggcgc
      361 ccgggtgcgc gacgcgatcg cggcgctggc cgcccggcac gcggcgatcg gcaagctgcg
      421 cggccggttc cgggtcgcgg tcaaccagga gttcagcgac gacgaccgcg cgctggccga
      481 cggcgacgag ctcgcgctga tcccgccggt cgccggcggc ggcgatccgc ccgcgcgcca
      541 cgtccagctg ctcgcgacgc cgctgtcgct cgatcgctgc atcgccgcgg ttgccggggc
      601 cacgctgggc ggcatcgtca cgttcaccgg catggtgcgc cgcgacagcc acggcgcggt
      661 catcgatcac ctggagtacg aggcctacgg cgcgatggcg gtccaggaga tgacgcggct
      721 gtgcgacgag atcgaggccg agatccccgg ggtccggctc gcggtcgagc accgggtcgg
      781 ccggctggag gtcggcgagg tcgccgtcgt gatcgcggcg gcggcgccgc accgcgccga
      841 ggcgttcacc gcgtgccgcg cgatgatcga ccggctcaag cagcgggtgc cgatctggaa
      901 gaaggaggtc ggcgaggacg gcgccgagtg ggtcgggctc ggtccgtagc gccgggccta
      961 tcgcgtcggc ccgtcgcgtg aaatgatgcg cggtgcgaat gatctggcac ggccgcgggg
     1021 gtttccggca tccgcatcga gcgctgatcg cagcgctcgc gtcgctcacc gtgctcacgg
     1081 ccttctcgcg ggcgcccgcg cgcgccgctc cggccctggt ggtgaacgag ccgtcgcccg
     1141 ggcagcccgc cggccagtac gcgctgcagg tccaggagga cggcaccgcc gtgctgcacc
     1201 tgcagtgggc cgccgccgat gcgaagccgc acgcgatcga tctggcgctg accgcgttca
     1261 cggcgaccgg caagcctgcg atcatcgtcg gcttcgacgc cggcaagccg gcggcgctgc
     1321 cgtacctcca gggcgtcgag gtgccggcgg acggcgcggc gttccggctg atcgccagcg
     1381 ggctggagcc cggcgtgagc tacggcggct cgctgaccgc gatcgtcgac cggcaaccga
     1441 cgacctggaa cctcgcgctg atccggccgg gcacgctgcc gctgttccgc tgcccggacg
     1501 ccgcgcagac cgtccggccc gatgactcga tcgtcctcga cgtgctgcgg gcgacgccgt
     1561 cgggcgcgct gaccgtcggg ctgcggctgt cgccgttcac cacgccggac agcgagcgcg
     1621 gcgacatcgg cttcccgtcg ggagccggcg gggcgcagct ggtcgacgag ctgcgcggcg
     1681 tccaggtcgc gggcggccac ctgacggtcg cactgcgcac ccgcgggctc accgagggcg
     1741 tcgcgtacag cggcaagctg acgctggcat ccggcggcgg ggacacgcag agctgcccgc
     1801 tcggcatcgt cctgcccaag ctgccgaggg gcgatctggt ggccgatcgc ccgagcttca
     1861 ccgccacggt caccgcgccc tggtcgggca ccgccgacac gagcctgtcg ctgcgcctgt
     1921 tcgagaagac ccgggcgcgg ccgctccacg ggatcaccgt ggcgctggac ggcaccgccg
     1981 agtcgccgca cggcggattc gacctcgacc gcaacacgac gttccagatc aacggcggcg
     2041 cggcggcgcg cctgacccgg ctcccgcccg aggacctgcg cgatccggcg cgcgcgatcc
     2101 cgccgggcgg ccagatggag gtgcggctcg cgctgcacga cctcgaggtc ggcaagtacg
     2161 ccttcgcgct gcggttcaac gccgtgaacg ccaccggccc gacccagcgg atcgagatca
     2221 ccgtgaacgt gcggcacggc tggccgtggg cggtgctggc ggtcgtggcc gcgctgctgc
     2281 tgtcgttcgt gctcaccaag agcatcgtcg gctggcgcaa gcggctcgcc ctgcggctgc
     2341 gcatcgacca gctgcgcgac cagagcttcg tcgagcactc ggacctcgcg atcgccgtgt
     2401 tcctgcgcac cgtgctcgac cagaccgagg agctgctcga gcgccacccg ctgctcccgc
     2461 cgccggacag catcgacgag tacatcacgc gcgccgagcg ggtcgcccgg atcctgatct
     2521 gctacagcag cgtcgtcgac acgctgcgcg ccacccggtg cgccgagcgg atcaagcact
     2581 actaccggga cgcgatcgcc gacgcgatcc accgcgtcgg gccggcctcg ctcgaccagg
     2641 ccaccgccga cgcgatcgtc gaccagctga ccgcgaccga gaaggcgctc gccgagccgt
     2701 tcccgtcgta ctggacgaac atcacggacc ggggcaggct cctggcgcag cagctccacg
     2761 acgcccgcca gacgctccgc gcgctggcgc agatcgacaa gctggtcgac acgctcggcc
     2821 acccggagca gctcaccgct ccgaggccgg acgatctgcg cgtcgcggcg tacgaccggc
     2881 tgtactggtt cggccggctg ctgtactcgc ggcgcgatga tcagacgcac gtcgaccagc
     2941 tggtccagca ttgcaagccg tgccagggcc acgacgactt cccggacgtc gacctcgatg
     3001 gtctgttccg ggacgccgac cgccgggcgt gggagcggct caaggccgag atcggcggcg
     3061 cgcagatcga gcaggtgcag cccggccagg gcccggaagc gctgcacccg ctccggttcc
     3121 tgctgcggtt cgagaatcgc gggctcgcca gcagctactt cgtgaacaac ctcctggcct
     3181 acgagtggca gttcgagttc acgccggaca agccggtccg gcgcatgccc cggatcgact
     3241 cgccggtccg ggtcaaccag ccccgggtca cgatgtacgt gcccgcggcc ggcaagctgt
     3301 ccgtgcgcgc ccggatcttc tggccgtcgg cgagcccgtc cgagcccccg ctggagctgg
     3361 agagcaagca gttcctcgtc ggcccgaacg aggacctccg gctgctcggc cacctggagg
     3421 cgagcacggt cgtgcacttc ggcatgatgg cggcggtcgc gatcgccacc ggcctgccgg
     3481 cgctgtactt cgacaacccg acgttcggct cgtacgccga ctacgtgaag atcctggcgt
     3541 gggggatcgg tatcgatcag ggcaagaacc tgatccagct ggtccgctcg gtcccacccg
     3601 acgccccgcc ggcgacgtga ttcactgcgg ggagccg
//
LOCUS       DASVOR010000018         3872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_292474,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000018 DASVOR010000000
VERSION     DASVOR010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292474"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            385..2283
                     /locus_tag="VF516_00415"
     CDS             385..2283
                     /locus_tag="VF516_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106150.1"
                     /translation="MWMVVVALAMTGCPPPAPPITYHNNASRTGWAGLEGTLTPANVT
                     RSTFGLIAHTELDGQVDAQPLVALRRRIAGGTHDVAYVVTSRNTVYAIDARSGVVLNR
                     VNLGPPVPMPLQCNNNDATVGITSTPLLDVDNRALYVMAYVLPSPNAAPIYQLHALDP
                     ATLADRAGSPVTVTASHVLTDHTVRRFDASVQRQRPALLYANGNIYAGFGSFCDYSPE
                     ASRGWVLGWKASTLAPLARNVLLDRLGPTPAYTLWDGTTHQPFYLAAVWMSGFGLAAD
                     EHGDVYFVTGNGNGLTYDGSASLQESAVKVSRDLSTVLDYFTPSNAVTPPDGLDPTDN
                     DFGSGGLMVLPAQPGRVPHLAVAAGKDGRMFLLDRDNMGKFHNPDRPASVDIGGCWCG
                     PSYFLGADGIGRVVTSGGNQVKTWKVNTAGTPTLVLEATSPALDSGQDPGFFTTVSSG
                     LTAAGTQIIWAVGRPTGAAHRITLYAISGTPVGGTLPLLWSGVAGSWPNTGGNANLVP
                     TVVAGKVYVASYRELAIFGLLPGGRAGISPEGLIHDVPPEDQPPGATIWGTVREVGER
                     TLVIQLRDGKLLSVDLTPAQRAGAIYLPKVGGYAAVRGALNDRGVLEASAISRAKSPA
                     SWGVDKLR"
     gene            complement(2280..>3872)
                     /locus_tag="VF516_00420"
     CDS             complement(2280..>3872)
                     /locus_tag="VF516_00420"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8106151.1"
                     /translation="PIPTQGRDSPLPVVRAREILATASDRDTVLLTLLRAARARARWA
                     GLLTVQGGAAIGRVALAEPGIDAAAINTVLIPLDSVSAFRTVVNNHQPHIGPLVSGDP
                     GIDAMVLRMGGTMPPSALILPIVLRDRVVALVVAHRLHSDLKLVDVTELLPMANAASE
                     AIGRLIVRHKSAGYRAPSDPGIVQVEAEMIDTKRISRGAEASWRAPSSPTPAPRLEHG
                     VEMSIEAGEPRPIDEVLSDIERANEHEAGDAISEAVERAPETLRALARRFPGKLRVDR
                     FAVTGRPLRAAQYGGLLELVIRLGGAAAELLIDKMSVAQRDVRFYATVCAAELRPRNA
                     VFALADRLFDQDYGVRATAIEALAGYPASELGHALTRARRAVHSTDPDVVAAASSALT
                     TLGDVDAIPDLIGVIERADRGGEHARRALTALTAQDFGASERKWRKWYDSARRRHRVE
                     WLIEGLSHKEDAIRETAINALRRLTGEYFGYHHDLPRKEREAAAERWAAWWRDAGHRR
                     FSIGPENERHRPTAELPPRRDS"
BASE COUNT          528 a         1352 c         1452 g          540 t
ORIGIN      
        1 tcgccgcggc cggctacgcc gagttcgacc cggtgtcgcc caacgacagc gagccgggcc
       61 gcgagcagaa ccgccggatc gagatcatcc tggtgcccaa cctcgaggag ctgccgagcc
      121 tgaccggcaa ccagccgtag ctcggaccag ccgagccggg cccgtgtcgt gggcgcgcag
      181 ctcgacgcgt gcgcttgcgc cacagctcgc gtgccacacc ttcgaacgat tcatgaaggt
      241 cgtcttacgg tccagaaatc ggtgggtgac cggcctcctt cccccccatc ctgagaagga
      301 gtcaggaggg taccgatggt ggatggaggc ggagggaaga cgagctctgg cgcgttgcgc
      361 gagggcagag gacgtgcgcc gggcgtgtgg atggtggtgg tcgcgctggc gatgaccgga
      421 tgtccgccgc cggcgccgcc gatcacgtat cacaacaatg cgtcgcgcac cggctgggcc
      481 ggcctggaag ggacgctcac gcccgcgaat gtcacgcggt cgacgttcgg cctgatcgca
      541 cacaccgagc tcgatggcca ggtcgatgcc cagccgctcg tcgcgctccg gcggcggatc
      601 gcgggcggca cgcacgacgt cgcgtacgtc gtgacctcca ggaacacggt ctatgcgatc
      661 gacgcgcgtt cgggggtggt gctgaatcgc gtcaacctcg ggccgcccgt cccgatgccg
      721 ctccagtgca acaacaacga cgcgaccgtc gggatcacca gcacgccctt gctcgacgtg
      781 gacaaccggg cgctctacgt catggcctat gtcctgccgt cgccgaacgc cgcgccgatc
      841 taccagctcc atgccctcga ccccgcgacg ctcgccgacc gggcgggctc gccggtcacg
      901 gtgacggcct cccacgtgct caccgatcat acggtccgcc ggttcgatgc ctcggtgcag
      961 cggcagcgcc cggcgctcct gtacgccaac ggcaacatct acgccggctt cgggagcttc
     1021 tgcgactaca gccccgaggc gtcgcgcggc tgggtcctcg gctggaaggc atcgacgctg
     1081 gccccgctcg cgcggaacgt gctgctcgat cggctcggcc cgacgccggc ctacacgctg
     1141 tgggacggca cgacgcacca gccgttctac ctggcagcgg tgtggatgtc cggcttcggg
     1201 ctcgcggcgg acgaacacgg agacgtgtac ttcgtgaccg gcaatggcaa tggcctgacc
     1261 tacgacggct cggccagcct gcaggagagc gcggtcaagg tcagccgcga tctgtcgacg
     1321 gtgctcgact acttcacgcc gtcgaacgcg gtgacgccgc ccgatgggct cgaccccacc
     1381 gacaacgact tcggatccgg cggcctgatg gtgctgccgg cgcagccggg ccgcgttcct
     1441 cacctggcgg tggcagcggg caaggacggc cggatgttcc tcctcgaccg ggacaacatg
     1501 ggcaagttcc acaacccgga tcgcccggcg agcgtggaca tcggcgggtg ctggtgcggg
     1561 ccgtcgtact tcctgggggc cgatggcatc gggcgcgtgg tgaccagcgg cggcaaccag
     1621 gtcaagacct ggaaggtgaa caccgccggc acgccgacgc tggtgctcga ggcgacctca
     1681 ccggcgctgg actccggcca ggaccccggg ttcttcacca cggtgtcgtc gggcctcacc
     1741 gccgccggga cccagatcat ctgggcggtc ggacgcccga ccggcgcggc gcatcggatc
     1801 acgctctacg cgatcagcgg caccccggtc ggtggcacgc tgccgctgct gtggtcgggg
     1861 gtggcgggga gctggccgaa caccggcggc aacgcgaacc tggtgccgac ggtggtcgcc
     1921 ggcaaggtgt acgtcgcgag ttaccgcgag ctggccatct tcgggctgct ccctggcggg
     1981 cgagcgggga tctcgccgga gggcctgatc cacgacgtac cgcccgagga ccagccgccg
     2041 ggggcgacga tctgggggac ggttcgcgag gtcggcgaac gcacgctcgt gatccagctc
     2101 cgcgacggga agctcctgag cgtggatctg acgccggccc agagggcggg cgcgatctac
     2161 ctcccgaagg tggggggcta cgcggccgtg cgcggcgcgc tgaacgaccg cggcgtgctc
     2221 gaggcgagcg cgatctcccg ggccaagagc cctgcgagct ggggggtcga caagctgcgc
     2281 taggaatcgc ggcgcggcgg cagctcggcg gtcggccggt ggcgctcgtt ctcggggccg
     2341 atcgagaacc ggcgatggcc ggcgtcgcgc caccacgcgg cccagcgctc ggcggcggcc
     2401 tcgcgctcct tgcgcggcag gtcgtggtgg tagccgaagt actcgccggt gagccggcgc
     2461 agggcgttga tcgcggtctc gcggatcgcg tcctccttgt ggctgaggcc ctcgatcagc
     2521 cactcgacgc gatgccggcg gcgcgcgctg tcgtaccact tgcgccactt gcgctcgctg
     2581 gcgccgaagt cctgggcggt gagcgcggtc agcgcccggc gggcgtgctc gccgccgcgg
     2641 tcggcgcgct cgatcacgcc gatcaggtcg gggatcgcgt cgacgtcgcc cagcgtggtc
     2701 agcgcgctgc tcgcggcggc gacgacgtcg gggtcggtcg agtgcacggc gcggcgggcg
     2761 cgggtcaggg cgtggccgag ctcggaggcg gggtagccgg cgagcgcctc gatcgcggtc
     2821 gcgcgcacgc cgtagtcctg atcgaacagc cggtcggcga gcgcgaacac cgcgttgcgc
     2881 ggccggagct cggcggcgca caccgtggcg tagaaccgga cgtcgcgctg ggcgacgctc
     2941 atcttgtcga tcaggagctc ggcggcggcg ccgcccagcc ggatcacgag ctcgagcagc
     3001 ccgccgtact gcgccgcgcg cagcggccgg ccggtgacgg cgaagcggtc gacgcgcagc
     3061 ttgccgggga accgccgggc gagcgcgcgc agcgtctcgg gggcgcgctc gaccgcctcg
     3121 ctgatcgcgt ccccggcctc gtgctcgttg gcgcgctcga tgtcgctcag cacctcgtcg
     3181 atcgggcggg gctcgccggc ctcgatcgac atctcgacgc cgtgctcgag ccgcggcgcg
     3241 ggcgtgggag agctcggcgc gcgccagctg gcctcggcgc cgcgcgagat ccgcttggtg
     3301 tcgatcatct cggcctcgac ctggacgatc ccggggtcgc tcggtgcgcg gtagccggct
     3361 gacttgtgcc tgacgatcag ccggccgatc gcctcgctcg cggcgttggc catcggcagc
     3421 agctcggtga cgtcgaccag cttgaggtcg ctgtgcaggc ggtgggcgac gaccagcgcg
     3481 accacgcggt cgcgcagcac gatcggcagg atcagcgccg acggcggcat cgtcccgccc
     3541 atccgcagca ccatcgcgtc gatcccgggg tcgccggaga ccagcggacc gatgtgcggc
     3601 tggtggttgt tgaccaccgt gcggaacgcc gacaccgagt cgagcgggat cagcacggtg
     3661 ttgatcgcgg cggcgtcgat cccgggctcg gcgagcgcga cgcggccgat cgcggcgccg
     3721 ccctggacgg tgagcaggcc ggcccaccgg gcgcgcgccc gcgcggcacg cagcagggtc
     3781 aggagcacgg tgtcgcggtc gctcgcggtg gcgaggatct cgcgggcgcg gacgaccggc
     3841 agcggtgagt cgcggccctg ggtcgggatc gg
//
LOCUS       DASVOR010000019         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_204761,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000019 DASVOR010000000
VERSION     DASVOR010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204761"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1723)
                     /locus_tag="VF516_00425"
     CDS             complement(<1..>1723)
                     /locus_tag="VF516_00425"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8106152.1"
                     /translation="AAPATPPAAVAPKNEKPKNEKPKNEKPKNEKPKNEDDPPAGPVG
                     YDVKKAFGAIARIHTTVDELTPQQRARLDALIARYNARTRKSKEYTAKHRGHMADPRV
                     VNGFRPLTKELTYQIVIERSRGSRLWDIDGNEYVDVLSGFGMSLFGWQADFIRAAIHE
                     QIERGYEIGPQHVLSGEVAELFCEVTGADRAAFCNTGSEAVMGTMRIARTVTGRSLIA
                     IFTGAYHGIFDEVIVRGTRKLKSIPAAPGIMPSASQNVLVLDYGTPESLEILRQRAGE
                     LAAIVVEPVQSRRPDFQPVEFLRELRALTEASGTLLIFDEVVCGFRAHPRGAQGLFGI
                     QADLASYGKVVGGGFPIGVIAGKRAFMDALDGGHWEYGDDSVPTVGVTYFAGTFVRHP
                     LALAAARAALIHMRDAGPALQAGLTAKTQAMVGEINRLMAEAGAPFKLNTFASLWRNV
                     FTEDLPYGDLIYAMLRDRGIHILDNFPCFLTTAHTDEDVAAIVAAYQAAASEMQASGL
                     FPAARTAIAVAAEAGVPGTRSSPSTEPQREVWLAARLGPEASLAYNESVSLHLRGELD
                     VAALRHAV"
BASE COUNT          232 a          596 c          628 g          267 t
ORIGIN      
        1 caccgcgtgg cgcagcgccg ccacgtcgag ctcgccgcgc aggtggagcg acaccgactc
       61 gttgtacgcg agcgacgcct cggggccgag ccgggcggcg agccagacct cgcgctgcgg
      121 ctcggtcgac ggcgagctcc gcgtcccggg aacgccggcc tcggcggcga ccgcgatcgc
      181 ggtgcgggcc gccgggaaca acccgctggc ctgcatctcg ctcgccgcgg cctggtacgc
      241 cgcgacgatc gcggcaacgt cctcgtcggt gtgcgccgtg gtcaggaagc acgggaagtt
      301 gtcgaggatg tggatgccgc ggtcgcgcag catcgcgtag atcaggtcgc cgtacggcag
      361 gtcctcggtg aacacgttgc gccacagcga cgcgaacgtg ttgagcttga acggcgcgcc
      421 ggcctcggcc atgagccggt tgatctcgcc gaccatcgcc tgggtcttcg cggtcagccc
      481 ggcctgcagc gcaggcccgg cgtcccgcat gtggatcagc gcggcgcgcg cggcggcgag
      541 cgcgagcggg tggcgaacga acgtgccggc gaagtaggtc acgccgacgg tcgggaccga
      601 gtcgtcgccg tactcccagt ggccgccgtc gagcgcatcc atgaacgcgc gcttgccggc
      661 gatcacgccg atcgggaacc cgccgccgac caccttgccg tacgacgcga gatcggcctg
      721 gatgccgaac aggccctgcg cgccgcgcgg gtgggcgcgg aacccgcaca ccacctcgtc
      781 gaagatcagc agcgtgccgc tggcctcggt cagcgcgcgc agctcgcgca ggaactcgac
      841 cggctggaag tcgggccgcc ggctctgcac cggctcgacc acgatcgcgg cgagctcgcc
      901 ggcgcgctgg cgcaggatct cgagcgactc cggtgttccg taatcgagca cgaggacatt
      961 ctgtgaggcc gacggcatga tcccgggcgc cgccgggatg ctcttcagct tgcgagtgcc
     1021 gcggacgatc acctcgtcga agatcccgtg gtaggcgccg gtgaagatcg cgatcaggct
     1081 gcggccggtg acggtgcgcg cgatccgcat cgtccccatc accgcctccg agccggtgtt
     1141 gcagaacgcg gcgcggtcgg cgccggtgac ctcgcagaac agctcggcga cctcgccgga
     1201 cagcacgtgc tgcgggccga tctcgtagcc gcgctcgatc tgctcgtgga tcgcggcgcg
     1261 gatgaagtcg gcctgccagc cgaacaggct catgccgaac ccggacagca cgtcgacgta
     1321 ctcgttgccg tcgatgtccc acaggcgcga gccgcgcgag cgctcgatga cgatctggta
     1381 ggtcagctcc ttggtcagcg ggcggaagcc gttgaccacg cgcgggtcgg ccatgtggcc
     1441 gcggtgcttc gcggtgtact ccttcgactt gcgcgtgcgc gcgttgtagc gagcgatcag
     1501 ggcgtcgagc cgggcgcgct gctgcggggt cagctcgtcg acggtggtat ggatacgcgc
     1561 gatcgcgccg aacgccttct tgacgtcgta gccgaccggg ccggcgggcg gatcatcctc
     1621 gttcttgggc ttctcgttct tgggcttctc gttcttgggc ttctcgttct tgggcttctc
     1681 gttcttcggc gcgacggccg ccggcggggt ggccggcgcc gcc
//
LOCUS       DASVOR010000020         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_321689,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000020 DASVOR010000000
VERSION     DASVOR010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_321689"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="VF516_00430"
     CDS             <1..195
                     /locus_tag="VF516_00430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106153.1"
                     /translation="PVMDGYELARRLREQLGPDKLRLIAVTGYGQDSDRARAREAGFD
                     YHLVKPVALDALMPLLVFGG"
     gene            complement(214..813)
                     /locus_tag="VF516_00435"
     CDS             complement(214..813)
                     /locus_tag="VF516_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106154.1"
                     /translation="MVRLVAIAILLASTAAHADSDTEGPPGQAVPDTAAAKSEDTALG
                     LSAVGPFASAGVLMLGVKLGNGPEAGTIRKGVGGVLVFAGGAGILLGPSFGEFYAGKY
                     LTGGMGLRLGGLGVMGLGAIAAALARGFDCRDLDCPKNPNTSGADAVIVVGAVLYGAG
                     WIYDVANARATVREYNRAHGSLRAMLVPTGNGVAVAGTF"
     gene            1685..2245
                     /locus_tag="VF516_00440"
                     /pseudo
     CDS             1685..2245
                     /locus_tag="VF516_00440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
     gene            2402..>2587
                     /locus_tag="VF516_00445"
     CDS             2402..>2587
                     /locus_tag="VF516_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106155.1"
                     /translation="MRLTQVLSNLLTNAQKFTPNGGRVTVSLEWLGARAVIRVRDTGI
                     GIEPSQIPALFEPFVQAA"
BASE COUNT          384 a          914 c          910 g          379 t
ORIGIN      
        1 ccggtgatgg acggctacga gctcgcgcgc aggctgcgcg agcagctcgg ccccgacaag
       61 ctgcgcctga tcgcggtgac cggctacggc caggactccg accgtgcacg cgcccgcgag
      121 gccggcttcg actaccacct ggtcaagccg gtcgccctcg acgcgctgat gccgctgctg
      181 gtgttcggcg ggtgacggcg gccatgcggg gtcctagaac gtaccggcga ccgcgacgcc
      241 gttgcccgtc ggcacgagca tcgcgcgcag cgagccgtgc gcgcggttgt actcgcgcac
      301 ggtcgcgcgt gcgttcgcga cgtcgtagat ccagccggcg ccgtacagca ccgcgccgac
      361 gacgatgacc gcatccgcgc ccgacgtgtt cgggttcttc gggcaatcga gatcgcggca
      421 atcgaagccc ctcgcgagcg ccgcggcgat cgcgccgagc cccatcaccc ccagcccgcc
      481 gaggcgcagg cccatcccgc cggtcaggta cttgcccgcg tagaactcgc cgaagctcgg
      541 gccgagcagg atgcctgcgc cacccgcgaa caccagcacg ccgccgacgc ctttccggat
      601 cgtgccggcc tcggggccgt tgccgagctt cacgccgagc atgagcacgc ccgccgacgc
      661 gaacggcccg accgcggaga gcccgagcgc ggtgtcctcg ctcttcgccg ccgccgtgtc
      721 cggcacggcc tggccgggcg gcccctcggt atcgctgtcg gcgtgcgcgg cggtcgaggc
      781 gagcaggatc gcgatcgcaa cgagtcgaac catgatccga gcgtagagcg cctcgcgcgc
      841 tgccaccggg acggttcacg cgatgttcac gtcgccgcaa gtagccggac gagcctgtct
      901 ttccaggagt cacatcgggg tgctgtggcg cggcgtcggg cgcgccgtag ccgccgccgc
      961 ccggggtctc gatcgcgaac acgtcccccg ccgccatctc gacctggccg aggtggccga
     1021 gccgctcgac ggcaccgcca ctccgctcga cctggttgac ccccacggcg ccggcgtgtc
     1081 caccggccag ccccggcgga cctgcgacgc gatagtgctc cgggctgtcc gacaacagct
     1141 tgtgggtgtg cagtcggccg ccggtcgtga gcctcataat ctatctcgcg gagagtacag
     1201 tagttcttgg agggcacgtc cttccgctcg gccagagagc gcgcgcgtgc gcacggtagg
     1261 tccagtagcc ggtgcgcatc ggcggggcgg cgcccctggg ccgatctccg gagaacagga
     1321 gcgggaggac gctgcgaccg ggataatcct gaggaacccg cgagcaatgg accttgcgct
     1381 tccgagcgag gatctctgat accccttctg agcctttgtc ttgtcgccgg atcgaggata
     1441 gaaaggatag caaggtacgc tatgttcctg caccgacggg agatcgcgtc atgaccgagc
     1501 gccggcgctc cgacctacgc tcgcgggcgg tggcgaagat cgcgcggcgc cagaaaaagc
     1561 cggcggcgcc gagcttgcgg tccgccgagc tggagatcca ccggatcgag ctggatctcc
     1621 agagcgagga actgcgcacc gcccgtggcc agacggaggc tgcactcgcc cgctccacca
     1681 ggtattcgac ttcgcgccga tcgggtacgc cgtgctcgac accggccagc agatctgcga
     1741 gatcaaccac gtgggcgccg cattgcttgg cgccgagcgc ggccggctgg tcggcaggca
     1801 gctcgccacc tacgtgatac gcggggggct cgtggcgttc cagtcgctgc tggaccaggc
     1861 gcgcggggtg acccactcgg tgcatgccga cctggagttc ctgcgtggcg atcagccttg
     1921 gctcgcccgg gtcaccgccg cggctctgcc cggcggcgag gccgcgctgc tgatcgcgtt
     1981 cgacgacatc acggacgcaa agcgcaagga ggccgagctg gcgctcagcg agcatgccct
     2041 gcgcgaggcc aaccggcgca aggatgagtt cctcgcgatg ctgtcgcacg agctgcgcaa
     2101 cccgctcgcg ccgatccgga actctctcgc ggtgctgcgc ctggtcgtgc ccgcgtccga
     2161 ccgggtgacc cggatgctcg acatcgccga gcgatcggcg ggccagctga cccggatgat
     2221 cgaggacctg ctcgacgtca cgcggatcac cagcggcaag atccagcttc agcgcgagcc
     2281 agtcgagctg gtcggcctgg tgtcgagcgc ggtcgaggat gtccggctcg cgttcgagcg
     2341 ccggaagctg cagccggagg tcgcgctccc gggcggcgag ctgtggacgc tcggcgatcc
     2401 ggtgcggctc acgcaggtcc tgagtaacct cttgaccaac gcccagaagt tcacaccgaa
     2461 tggcgggcgg gtgacggtgt cgctggagtg gctcggtgcg cgggcagtga tccgcgtgcg
     2521 ggacactggc atcggcatcg agccgtcgca gatccctgcg ctgttcgagc cgttcgtcca
     2581 ggcggcc
//
LOCUS       DASVOR010000021         5337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_146312,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000021 DASVOR010000000
VERSION     DASVOR010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5337
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146312"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF516_00450"
     CDS             <1..199
                     /locus_tag="VF516_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106156.1"
                     /translation="EEKTLTGSYFGSARPREDFPKMLSLYAAGKLKLDELITRRYKID
                     EAPQAFADLESGKNARGVIVF"
     gene            290..451
                     /locus_tag="VF516_00455"
     CDS             290..451
                     /locus_tag="VF516_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106157.1"
                     /translation="MKHTILFLAANPAGTDRFALDREAHDIHAELERSGHRDRFELVD
                     RLLRRAGGA"
     gene            complement(681..1940)
                     /locus_tag="VF516_00460"
     CDS             complement(681..1940)
                     /locus_tag="VF516_00460"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX8106158.1"
                     /translation="MLDYLERVLGYAPRTAQDRLRVARALSDLPRITAALSAGDLPFS
                     AVRELTRVATPATEASWVAAATGKNLRQIEELVADHRPGDRPDDPPDPEARTHVVRFE
                     LSGATFALLRQARIVLDDAHGTNLPDDAFVAALCGAVLDGASQTETSGRAKFQIAITV
                     CQRCGHGWQDGAGARVALDPAAVDRALCDAQHIGSIDGDGPERAHQDIPPSVARFVWR
                     RDGGRCRVDGCRSSRGLELHHVIHRADGGGHDASNIVLLCSACHLAHHRGALTVTGTA
                     NELQIRRPGEAAATGTQEDAGGVAGLPAHSAHVGAALRPRGSMMEVDASVAAGGALTT
                     TDPDAHVGATSSAGPVEDTSVRASGARRLDAAIFRTQAKAALTGLGWKPAIAHAAVAA
                     AAEAHGEDVTLEHLIFEALRRCPTPRA"
     gene            2713..3057
                     /locus_tag="VF516_00465"
     CDS             2713..3057
                     /locus_tag="VF516_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106159.1"
                     /translation="MTEQRMPMTDTAGLLSMPPVDCRWPIHRAVDSGTQQSTSLFGDR
                     HPHRWSVVGARWSLVGTGIGGRRSVPAIGLGGSALLVGERYWRSVLARWSASVISVGR
                     RLAGPDIPDPAR"
     gene            3147..3614
                     /locus_tag="VF516_00470"
     CDS             3147..3614
                     /locus_tag="VF516_00470"
                     /inference="COORDINATES: protein motif:HMM:NF018748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8106160.1"
                     /translation="MAPTIICPPFTEETAIRKVRMAEDAWNTRDPARVALAYTEDSKW
                     RNRSEFFQGRPAIIAFLERKWQRELDYRLIKEIWTYGSNRISVRFQYEWHDQDNHWFR
                     AHGNEQWEFDDSGLMRRREASINDVPIQAADRKFLWPQGPRPADAPGLTELGL"
     gene            3703..4623
                     /locus_tag="VF516_00475"
     CDS             3703..4623
                     /locus_tag="VF516_00475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX8106161.1"
                     /translation="MARAAQPRPRRRPQGDDGDALGPWALDTVHNVDCERGLRALPAD
                     CLDVVVTSPPYWGQRGAGGLGAETDPRDYIARLAAMLAEAMRCLKPSGTLWLNVGDAY
                     NTPINWRAADHVYSSLGADGTGLPPTNSAYTKNRGRRRAFVRSDVGWLSYGNLLAIPY
                     RVVGAMCDRGFLFRGEVIWEKSRPMPEGLCRRPHRRHEGIYVFAKDEQHAFRTRPPVG
                     SVWKLRQTPNLTPHYSTFPVDLPMQCIRAAGLASRGAIVCDPFMGSGTTARAARMLGH
                     RFLGFELDPEICRLANDRAREVAPRSQRPR"
BASE COUNT          810 a         1883 c         1762 g          882 t
ORIGIN      
        1 cgaggagaag accctgaccg gcagctactt cggctcggcg cgaccgcgcg aggacttccc
       61 gaagatgctc tcgctctacg ccgccggcaa gctcaagctc gacgagctga tcacccgccg
      121 ctacaagatc gacgaggcgc cccaggcgtt cgccgacctc gagtcgggca agaacgcgcg
      181 cggcgtgatc gtgttctgat cggggaggcg ggggaatcct gcgatcgcgc cacgttgcgc
      241 gaacctgtcg tggagccggg tgctcccgtc gcctcaccgg ggtgccccga tgaagcacac
      301 catcctgttc cttgctgcca accctgccgg caccgaccgc ttcgccctcg accgcgaggc
      361 ccacgacatc cacgcagagc tcgagcgcag cggtcaccgc gatcgcttcg agctcgtcga
      421 tcggcttcta cggcgggctg ggggagcgtg aatcggtcga ggcggcattc ccgccagggg
      481 cgtgccgcga ttcgccttgc cggtctgtgc gacggctacc ggctgcggtt cgcggccccg
      541 cgggtctcga cgccagccgg ctggccgccg ccgcggaccc tcgatagcct tcgcatcgtc
      601 ggcgcggcgg ttccgaggcc ctcgagccgg ccccgcggcg atcgactgcg tgcgcgccag
      661 gtggcccggc gcaactcgcc tcatgctctg ggcgtgggac agcgtcgcaa tgcttcgaag
      721 atcaggtgtt ccagtgtcac gtcctccccg tgcgcctccg ctgcggcagc gaccgcggca
      781 tgcgcgatcg cgggcttcca gcccaggccg gtcagcgccg ccttggcctg cgtgcgaaag
      841 atcgctgcgt cgagcctgcg ggcaccgctc gcgcgaacgc tcgtgtcctc gaccggaccc
      901 gcggagctcg tcgcgcccac gtgggcgtcc ggatcggtag tggtgagcgc gccaccggca
      961 gccacgctcg catcgacctc catcatggaa ccccgtggcc gcagcgccgc gcccacgtgg
     1021 gcgctgtgcg ccggcaatcc ggcgacccca cccgcgtcct cttgcgtgcc ggtcgccgct
     1081 gcttcgcccg gccgacggat ctgcagttcg ttcgccgtgc cggtgaccgt cagcgctccg
     1141 cggtgatgcg cgaggtggca cgcgctgcac agcaagacga tgttcgaagc gtcatggccg
     1201 ccgccgtcgg cgcgatggat cacgtgatgc aactccagcc cgcgggacga gcgacaacca
     1261 tccacgcgac agcgaccccc gtcgcggcgc cacacgaagc gcgccacgct cggcgggatg
     1321 tcctggtgcg cccgctccgg tccatcgccg tcgatcgagc cgatgtgctg ggcgtcgcac
     1381 agcgcgcggt cgaccgcggc cgggtcgagc gcgacccgcg cacccgcgcc gtcctgccag
     1441 ccatgcccgc aacgctggca taccgtgatc gcgatctgga acttcgcgcg gccggaggtc
     1501 tcggtctgcg aggcgccatc gagcaccgcg ccacacagcg ccgcgacgaa cgcatcgtcc
     1561 ggcaggttgg tgccatgcgc gtcgtcgagc acgatgcggg cctgccgcag gagcgcgaag
     1621 gtcgccccgg agagctcgaa ccggaccaca tgcgtgcgcg cctcggggtc cggcggatca
     1681 tcagggcggt cacctgggcg atggtccgcg acgagctctt cgatctgccg caggttcttg
     1741 ccagtcgctg cggcgaccca tgaggcttcg gtcgccgggg tcgcgacgcg cgtcagctcg
     1801 cgcaccgccg agaatggcag atctccggcc gagagggcgg cggtgatccg tggcaggtcg
     1861 ctgagggccc gcgccacccg cagtcggtcc tgggccgtgc gcggggcgta gccgagcacg
     1921 cgttcgaggt agtcgagcat cgagaccatg ccgagcgggc gccagatctg caatccctcc
     1981 gcctcgcgca gccagcgagc ctcctcggca tcgagcgcgg cgcggcgccg ccggatcgat
     2041 cgcagcgcgc gatccaccgt cttccaatcg agcctggcgt ctggcgcggg agcgggcgcc
     2101 cacgcgagcg ccggcgcgcc ttcgatcact gcgttttcga ttccgccact cagcatgaca
     2161 gcctcccgct gttgtcgtgc atcgcttcta gcacacgagt tttcggcgcc atggcttgcg
     2221 cgctgcgcga cgatccggcg ttgccctcga gaaaatgtgg gtcgcctgcg atttcgctgt
     2281 cacagcgcac gccggcgcgc cctgagcatg atcaatgggc agcttctggc gcagtgctcg
     2341 atagatcgcc ggcaggtcgt caagtaaaat ctactccaca atgtgccggg tgccgttaaa
     2401 tgccattctg cacgcctcat ccgccgaata ccgtacatcg ccaacggccc atcactgcgg
     2461 agattggact tgcttcccga cggtggaccg tccatcgcgt ccttgatccg acctcggcgg
     2521 gtgagtcccc acaacagtcc cagctcacga gcgagcagtg cccgcgcctg cttgctctcg
     2581 atctgccagc cccacgcagc tgcgagatcc agcgcaccgc ggatctccgc tgcgctgccg
     2641 tgcgccatgg cgaaggaccc ccgcggatcg cgggaggtga actgcgtcca ctgtccgcac
     2701 tcgaggctgt cgatgaccga gcagcggatg ccgatgaccg ataccgccgg cctcctttcg
     2761 atgccgcctg tcgactgccg atggccgatc caccgggcag tcgactcggg cactcagcaa
     2821 tcgacgtcgc tgttcggtga tcgacacccg catcggtggt cggtggtcgg cgcgcggtgg
     2881 tcgctggtcg gtaccggcat cggtggtcgg cggtcggtgc cggcgatcgg cctcggtggt
     2941 tcggcgttgc tcgtcggtga acggtactgg cgatcggtac tcgcccggtg gtcggcctcg
     3001 gtcatcagcg tcggtcgccg gctggctggc cctgacatcc ccgacccggc ccgctgacat
     3061 ctccccaccg ctcgctacaa tcctcgtgcg ccgggcaccc cccagcgccc ggcttcgaag
     3121 cgagactcgc acgcaaggag agcgacatgg ccccgaccat catctgcccg ccattcaccg
     3181 aggagaccgc gatccgcaag gtccgcatgg ccgaggacgc gtggaacacc cgcgacccgg
     3241 ctcgcgtcgc gctggcgtac accgaggaca gcaagtggcg caaccgcagc gagttcttcc
     3301 agggccgccc ggcgatcatc gcgttcctcg agcgcaagtg gcagcgcgag ctcgactacc
     3361 gcctgatcaa ggagatctgg acctacggct cgaaccggat ctcggtccgc ttccagtacg
     3421 agtggcatga ccaggacaac cactggtttc gcgcccacgg caacgagcag tgggagttcg
     3481 acgacagcgg cctgatgcgc cgccgcgagg cgagcatcaa cgacgtgccg atccaggccg
     3541 ccgaccgcaa gttcctgtgg ccacagggcc ctcggccggc cgacgctccc ggcctgaccg
     3601 agctcgggct gtgaggcagg ccgccctggt gttccgtgga tgaaatattc caccggcagt
     3661 cgcgcgcttg cctcggccac ggacccgcgt tcacatgggg ccatggctcg cgccgcgcag
     3721 cctcggcccc gacgccgtcc acagggcgat gacggtgatg cgctcgggcc gtgggcgctc
     3781 gacaccgtgc acaacgtcga ttgcgagcgc gggctgcgcg cgctgccggc cgactgcctc
     3841 gacgtcgtcg tgacctcgcc gccgtactgg gggcagcgcg gggcgggcgg gctcggcgcc
     3901 gagaccgatc cgcgcgacta catcgcccgg ctcgccgcga tgctcgccga ggcgatgcgc
     3961 tgcctcaagc cgtcgggcac cctgtggctc aacgtcggcg acgcgtacaa cacgccgatc
     4021 aactggcgcg cggccgatca cgtgtatagc tcgctcggcg ccgacggcac cgggttgccg
     4081 ccgaccaact cggcgtacac caagaaccgc ggccggcggc gggcgttcgt gcgcagcgat
     4141 gtgggctggc tgagctacgg caacctgctc gcgatcccgt accgcgtcgt gggggcgatg
     4201 tgcgatcgcg gcttcctgtt tcgcggcgag gtgatctggg agaagtcgcg gccgatgccg
     4261 gagggtttgt gccggcggcc gcaccgccgc cacgagggca tctacgtgtt cgccaaggac
     4321 gagcaacatg cgttccgcac ccggccgccg gtcggctcgg tgtggaagct gcggcagacc
     4381 ccgaacctga cgccgcacta ctcgacgttc cccgtcgatc tgccgatgca gtgcatccgg
     4441 gccgccggcc tggcgtcgcg cggcgcgatc gtctgcgacc cgttcatggg cagcggcacg
     4501 accgcgcgcg cggcccgcat gctcggccac cgcttcctcg gattcgaact cgatcccgag
     4561 atctgccggc tcgccaacga ccgcgcccgc gaggtcgcac cgcgctcgca gcggccgcgg
     4621 tgaaccggcc gtgtcaggcg ccgcatgcac gagtcactcg ccgcgtcgcc gcgtcgccgc
     4681 gtcgctgcat gggagagttc gatcgaggac cggccgatga ccggccgatg aggttgcgcg
     4741 gccatctttc ggtcggcgcc tcatgacgca cattttgcta gatatcctcg acggatgtcc
     4801 tcttacgctg acaaagaacg taatatatat aacacaaata atttggtctg ttgaggtgcg
     4861 gcttccctga gatcgcctca tgatggcctc tccgctgtcg cgagtacgtg acatcacttg
     4921 catctcagtt tttcgatgct taagattcca atatattcaa ttcatcctca gattcttgtc
     4981 ggctggggcg aatttgcgtc tgtctccata ctgctgagct tcggctaact cgaattgcac
     5041 tcgtgacgcc gaaaaaccgg attgacattt gcggacacac agccgtagtg tttgtgccgt
     5101 cgaacgggtg aaccatcaat aaactggtat cccgattggc agtcgtccat ggtcttccct
     5161 gatcgtgcgg ggcgcatctc gccatgatcg tcggggatcg ttcgagctgg ctcacgacat
     5221 ggtgcgtcgt gcgcagtgtc catcttgtag ccggagaaac atgaaatgaa tcaaatgaca
     5281 tggattcgca gcggagtgct cgtgacaacc accgcagtcg gcggctggct cgctgga
//
LOCUS       DASVOR010000022         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_438628,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000022 DASVOR010000000
VERSION     DASVOR010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438628"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..1066)
                     /locus_tag="VF516_00480"
     CDS             complement(128..1066)
                     /locus_tag="VF516_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEX8106162.1"
                     /translation="MAFADLKNDFVFRRIFATHADLLCSLLNDLLERSGEHTIETIEY
                     LPSEQLPLAAGAKLSILDVRCKDRSGTTFVVEMQLIHVEGFINRVVYNACKAYPGQLQ
                     ESEKYVQLTDVVAISICDFELWPDDEQRAQGLPLVPMLSRWHMTERKSGNHGLLQVQY
                     VFLELPKLPRAADAARRRAVGVAVRARPRAARDPGGPGRWSVSPGPRAREQGDVHARG
                     ARRVSPGDRRDPPGAGAGRRQVARGPGRCQGGCRAGGAGGAWDPGAGLHSRAHRSLQG
                     PGDARPVDRACRHGGFGRGRPGVAGIVALEPAREPR"
     gene            complement(1173..>1688)
                     /locus_tag="VF516_00485"
     CDS             complement(1173..>1688)
                     /locus_tag="VF516_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106163.1"
                     /translation="FLEAGQSAASYQLWRSALRCYRRALELDLADREAVGRIVQLPPR
                     VIALADWHDYARALDSGLGTRGWPSLSCRAAQIVTGNLGAQVECPGTGVVMELMMTED
                     DLVETRPEARFAGMPLAMAMILLRRALWFAPRELATDPASIRVAFDGRPQVRLDELGD
                     WEPVGSTAQPR"
BASE COUNT          260 a          607 c          571 g          250 t
ORIGIN      
        1 cccgtccgcc tgggcggtct gcccccacag cagcgccgac gctgccatcc agatcacata
       61 gttgcgaagg cagatcatcg catcccgtcc aagcagcaag actccggccg tcgatgttgc
      121 agcgacgtta ccgcggttcg cgggcgggtt cgagcgcgac tatgccggcg acgccaggac
      181 gtcctcgacc gaagccaccg tggcggcacg cgcgatccac cggtcgagcg tcgccaggtc
      241 cttgcaagct tcgatgcgct ctcgaatgga gtccggcacc gggatcccac gcgccgccag
      301 caccgccagc acggcagcca ccttggcagc gacctggccc tcgcgccacc tggcgtctgc
      361 cagctcctgc gcctggtgga tctcgtcgat cacccggcga tacgcgtcga gctccccttg
      421 cgtgaacgtc gccttgttcg cgagctcgag ggcccggcga tacggaccag cgcccaggtc
      481 cgccgggatc tcgggcagct cgcgggcgtg cgcgaacagc cacgcccaca gctcggcgcc
      541 gggctgcgtc ggccgctcgc ggcagcttgg gcagctcgag gaacacgtac tggacctgca
      601 gcaacccgtg gttgcccgac ttgcgctcgg tcatgtgcca ccggctcagc atcggcacca
      661 gcggcaggcc ctgggcccgc tgctcgtcgt ccggccacag ctcgaagtcg cagatcgaga
      721 tggcgaccac gtcggtcagc tggacgtatt tctcggactc ctggagctgc cccgggtacg
      781 ccttgcacgc gttgtagacc acgcggttga tgaagccctc gacatggatc agctgcatct
      841 cgaccacgaa ggtcgtgccg ctgcggtcct tgcaccggac atcgaggatc gacagcttgg
      901 cgccggcggc gagggggagc tgctcgctcg gcaggtactc gatggtctcg atcgtatgtt
      961 cgccggagcg ctccagcaga tcgttgagca ggctgcacag caggtcggcg tgggtcgcga
     1021 agatccgccg gaacacgaag tcgttcttca gatctgcgaa cgccacatct gctccttgcc
     1081 agagcccggc acgcggcgca agcgagtgcg ggtctcgcgg agctaattgc ccttgccggc
     1141 ggacgattct cgaagcttcc gtccccgacc atctaccgag gctgcgcggt ggagccgacc
     1201 ggctcccagt cgccgagctc gtcgagccgg acctgcggcc ggccgtcgaa cgcgacccgg
     1261 atcgacgccg gatcggtcgc gagctcgcgc ggcgcgaacc acagcgcgcg gcgcaggagg
     1321 atcatcgcca tcgcgagcgg catccccgcg aaccgcgcct cgggccgggt ctcgaccaga
     1381 tcgtcctcgg tcatcatcag ctccatcacc acgccggtgc cggggcactc gacctgcgcg
     1441 ccgaggttgc cggtgacgat ctgggccgcg cggcagctca gcgacggcca gccgcgggtc
     1501 ccgaggccag aatcgagcgc gcgggcgtag tcgtgccagt cggcgagcgc gatcacgcgc
     1561 ggcggcagct gcacgatccg gccgaccgcc tcgcgatcgg cgagatcgag ctcgagggcg
     1621 cggcggtagc agcgcagggc gctgcgccac agctggtagc tcgccgcgct ctggccggcc
     1681 tcgaggaa
//
LOCUS       DASVOR010000023         8916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_438635,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000023 DASVOR010000000
VERSION     DASVOR010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8916
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438635"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF516_00490"
     CDS             <1..411
                     /locus_tag="VF516_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106164.1"
                     /translation="PTLAHPDMDHSFKSLFEESGKDLCLQPQWNDNSTVGEIDIDFHR
                     QLETIDHNRLDNSDPLTTSSGGGKNNNATYIACWGTPRDLPGYSGPMTVSQSDRGPVY
                     DFHGQRDMLGYPEVAHVLAGRGIAVATGDVAVPR"
     gene            734..931
                     /locus_tag="VF516_00495"
     CDS             734..931
                     /locus_tag="VF516_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106165.1"
                     /translation="MAISDVTHAASREVGARHSHVKSLTCVAIERWIVRTARRDAPGA
                     LDLADADGDDEDADAGEDLSA"
     gene            953..3913
                     /locus_tag="VF516_00500"
     CDS             953..3913
                     /locus_tag="VF516_00500"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEX8106166.1"
                     /translation="MTMGRHALQRLYLAEDLVRRRRADEYRRQVASQRAARIDPNPHQ
                     IDAVIFALERIPDGGCILADEVGLGKTIEAGLVIAQLLAEGAKRVLVVTPKALLGQWK
                     QELYALFGIDAREITRDAASFAGEGVFLATRDLVGSDAGAAALGASERFDLCVVDEAH
                     EVFASIYKRFDRGGSIREDSPYARIAGRLSTAIAGTPVLLLTATPLQNSLLELWGLVH
                     YVDPTGTLLGDLSTFRNLFCPTDDRILLEGQEHELQRRLGTVLQRTLRRQAQDFMREP
                     FVGRQARLFDYSMSPEERALYDDVTMYLLEPSLYAFRGKHRTLLLLGFHRRMASSVRA
                     LAASLEKVAERLRALRDRRAPDDAADADAILGDLEDDDDEPSADDAPDVREDERRAIA
                     AELARVESFIQRARALPTDSKAKALLSAIKLVAEQAEHGKSSGKVVVFTESLTTQEYI
                     RELLLADGVVGDSDITLFRGDNDTPRAAIAHARWFEEVGRQLPPESRPSPDVAIRLAL
                     VHEFRTRPHGIFISTEAGAKGLNLQFCDTVINYDLPWNPQRIEQRIGRCHRYGQRRDV
                     TVINFLARDNEAQKLTFDILSQKLDLFGTVLGATDAVLHRPGTLAPETLASGLGAEFE
                     TQLRRIYERARTLEEVERELRELRDSMEARRREFETALKRTEDVIQRRFDASVRATFR
                     RIQAEFPRELAEFDHHLEMVVTEYLDAVSVPYRMTRQDGGAILKLEDPSRLPASLQDL
                     PGCAIGSSSPGSPYGSLHLRHPLVVAALEHARASIQRTGSSVRVRVAEADDDLRGRRG
                     RMRLIRVLHRNFEITEHLVPVVLLEGDDAPLPADAARRILDAEIEDLPAPIGCGVAVE
                     ELDDAVDELLFHDTSEASRLEQPRFERTLEQIERFITDRVLLLERQRDAAVARLTKAQ
                     DARDHAIGAEQRNAAERAMHKAQTEIDSLDDKLRTLRAGDDERYRKWRRHTEERRYTP
                     PEIEHIFDAELEIV"
     gene            3934..5073
                     /locus_tag="VF516_00505"
     CDS             3934..5073
                     /locus_tag="VF516_00505"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEX8106167.1"
                     /translation="MALKLLHTADWHLGRRFPSFREADETKLTRARVDVLDKILAVAE
                     QRAVDAVLCAGDLFDEPSPDSEWWQPVAKKLATWRARIPMFLLPGNHDPLQPGSVYHP
                     GHGFRHALPPWVRVVDDDSFTARLNDVAVLHAKPCRSSAGQDDPALALPAREPGDPRI
                     RIGMVHGSTFDYVDCQTNFPISRDAAAQRGFDYLAIGDTHSFRVVPPDARPPVVYPGA
                     PEATSFDEPGAGKVVVVFVSRSRRVTLAPEPVAYWQWRDVKVTSLAELRDLRAEQLAR
                     TVLRLTVEARLSANEMEEAEAILRELGGTAASYGRAGILQLNRSKLTVDTRGIETQLS
                     DLPAVLRATVTRLKQLEATDDPKVAEYAQAALYHLYRLVRQERQP"
     gene            5070..>8916
                     /locus_tag="VF516_00510"
     CDS             5070..>8916
                     /locus_tag="VF516_00510"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8106168.1"
                     /translation="MRLVKLSVQRFQCIESAELELGPHLNILYGPNDLGKSSLAWAIR
                     AVLLLQHGSAHAERFSSWYAAAEPRVALTFCDDSGRYWRVTKTFGGSTGRSELESSKD
                     GQVFTMEEKARSVDEKLRVLLRWGLATPGGKGRTAVPESFLTHVLLADQAQDSVRKIL
                     FEPKLDKDLDESGRERLVAALGALAQDPLFKTILDQATESMQRAFTPKGQRKRSAGSP
                     WLEAIERSKALRAKKDQLEAQVKETARVEEHIKGLHGRHDSVAGELTEAEEAHRVAVR
                     QLEAQKQRDVLHGEVQSLQQRIRDAETMAKQIEVASGGATKLESDAAAARESARQAAD
                     RAAQVETELNAIRRQLDTVLHDDPDAERRMAELQAEVGRTESQAREALDVVSAARDAL
                     RTAGKLAANLVAEDAAVKQTSAIADAADAAAAVALRDAERARKFLADAQQRQRDARSH
                     DRAQARELRRKELENQALSLASKRARIEATLQHAMHIVELTARLVKTEADVRATRDQL
                     AAIHGEITTYETEHQELEAARAVLGQLHAYGQFRQLREELARATQASEDADRERRRAA
                     ELRERAERLRSEVRGGLPAAEQIQELDRLRQQLQVAEAALGGGLSVEIRPKRSLTLRA
                     TRDGATDPATTGSELVALTAKRSIALAIDDWLDIEITAGEEVARRTAAELRARWLREG
                     ESVLVEHGLASLDELRLTRSRSDAAARDIDDAIREAENAERRASLVGDADVATVREQL
                     MGAESELGAADREDLATRFARLGASWQTGLRTRSAELEARLASSRTRLEQKRAGVAKL
                     EAQLDVQQGEAARLEAEVERAQAELTGPPTVVVATCEADQQALAVEQRDIEHQIAGLA
                     TGTSEEEAAADAAVVAAGTALQTAEADAQAKQHTAQAARVAQVRASTWLEGIRTTARQ
                     LDAQGVWASRLDRGDASLPVDAWEAAVRDAEDQLEKARAAHARATAALTDARAAHDAA
                     VQGLRRKVQDAEDASRQARSRSDAAEALHKELIAQHTAAVVSLSELKVKIAGIHVDEL
                     RAEIAKRRTRMLELEPEMGRVNEAGITELDQKLGRLRAELRELDEELAKARGALEHVG
                     GAVVRERLAEIGQAIEQAEQREHEVEVEYEAWRLLHDTLRQAESTESAHLGRALAGPV
                     GSRFRQLTGGRYGDIELGAKLEAGGIHVAGGLREIAALSAGTQDQLATLLRVCIAQQL
                     RSAIILDDHLTQSDPARVAWFNTTLREAAESVQIILITCRPQEVLTADEVCSPGQVRR
                     ASADGRVHAVDLAAVIRRFD"
BASE COUNT         1514 a         3046 c         3162 g         1194 t
ORIGIN      
        1 ccgacgctgg cgcaccccga catggatcac tcgttcaaga gcttgttcga ggagagcggc
       61 aaggatctct gcctgcaacc gcaatggaac gacaactcga cggtcggcga gatcgacatc
      121 gacttccacc ggcagctcga aaccatcgac cacaatcgcc tcgacaattc cgatccgctc
      181 acgaccagct cgggcggcgg caagaacaac aacgccacct acatcgcttg ctggggaact
      241 ccccgagacc ttccgggtta cagcggcccg atgaccgtca gccagagcga ccgcggcccg
      301 gtctacgact tccacggtca gcgcgacatg ctcggctacc ccgaagtcgc gcacgtcctg
      361 gccggccgcg gcatcgccgt cgccaccggc gacgtcgccg tcccgcgctg acccttgagc
      421 ttggcacggc tcccgcggca agcagccggc tggcgcgcat catgtcgcca cgctcttgac
      481 gacgcagcaa tagagcggtc ccacagccaa gcgagcccaa tccgctgatg gagctcgagc
      541 tcacatgatg gtgcgagaag accggccaca atgtcccagg gcaccgcgga ctcgggacct
      601 tcggcctgcg agcgcccagc gcggctacac ggcaagcagc acctgcgagc gcgagatcga
      661 ggccgcgaag agctcgtggc acgccacatc gcctacactc cctgccgcga gccggccgtg
      721 attcgccgca gcgatggcga tctccgacgt gacccacgcg gcgtcgcgcg aggtcggtgc
      781 gcggcatagt cacgtgaagt cgctgacttg cgtggccatc gagcggtgga tcgttcgcac
      841 cgccaggcgc gacgcgccgg gtgctctcga cctcgcggac gcggacggcg atgatgagga
      901 cgccgatgca ggcgaggacc tcagtgcgtg atcgacgacg ggcgctcggg cgatgaccat
      961 gggacgacac gcactgcagc gcctgtacct cgcggaggat ctggttcgcc ggcgccgtgc
     1021 ggacgagtac cgccgccagg tcgcgtccca gcgcgcggcc cgcatcgatc cgaacccgca
     1081 ccagatcgac gcggtgatct tcgcgctcga acggattccg gacggcgggt gcatcctggc
     1141 cgacgaggtc gggctcggaa agaccatcga ggccgggctg gtgatcgcgc agctcctggc
     1201 cgagggggcg aagcgcgtcc tggtggtcac gcccaaggcg ctgctcggcc agtggaagca
     1261 ggagctgtac gcgctgttcg gcatcgacgc gcgcgagatc acccgggatg cggcctcgtt
     1321 tgccggggaa ggcgtgttcc tcgcgacccg cgacctcgtt ggcagcgacg ctggcgcagc
     1381 cgctctcggc gcgagcgagc gcttcgacct gtgcgtcgtg gacgaggcgc acgaggtgtt
     1441 cgccagcatc tacaagcgct tcgaccgtgg cggctcgatc cgcgaggact cgccctacgc
     1501 acggatcgcc ggccggctgt cgacggcgat cgcgggaacc ccggtgttgc tcctgaccgc
     1561 gacgccgctc cagaactcgt tgctcgagct gtgggggctc gtgcactacg tggacccgac
     1621 cggcacgctg ctgggtgacc tcagcacgtt ccgcaacctg ttctgcccca ccgatgaccg
     1681 catcctgctc gagggtcagg agcacgagct gcaacggcgg ctcggcacgg tgctgcagcg
     1741 aacgctccgc cggcaggcgc aggacttcat gcgcgagccg ttcgtgggac ggcaggcgcg
     1801 gctgttcgac tactcgatga gcccggagga gcgcgcgctg tacgacgacg tgacgatgta
     1861 cctgctcgag ccgagcctgt acgcgttccg cggcaagcat cggacgctcc tgctgctcgg
     1921 ctttcaccgg cgaatggcgt cgtcggtccg ggcactcgcc gccagcctcg agaaggtcgc
     1981 cgagcgcctg cgggcgctgc gggaccggcg tgcgcccgat gacgcggcgg acgccgacgc
     2041 gatcctcgga gatctcgagg acgacgatga cgagccatcc gccgacgatg cacccgacgt
     2101 gagggaggac gagaggcggg cgatcgcggc ggagctcgct cgcgtggagt cgttcatcca
     2161 gcgcgcccgc gcgctgccga cggacagcaa ggcgaaggcg ctgctcagcg ccatcaagct
     2221 cgtcgcggag caggccgagc acggcaagag cagtggcaag gtcgtcgtgt tcacggagtc
     2281 gctcacgacg caggagtaca tccgcgagct gctgctcgcc gacggagtgg tcggtgacag
     2341 cgacatcacg ctgtttcgcg gcgacaacga cacgccgcgc gcggcgatcg cacacgcccg
     2401 gtggttcgag gaggtcggcc gccagcttcc gcccgagagc cggcccagcc cggatgtcgc
     2461 gatccggctc gcgctcgtcc acgagttccg cacccgaccg cacgggatct tcatctccac
     2521 cgaggccggg gcgaagggcc tcaacctcca gttctgcgac accgtcatca actacgacct
     2581 gccgtggaac ccgcagcgga tcgagcagcg catcggccgc tgccaccgct atggccagcg
     2641 ccgcgacgtc accgtgatca acttcctcgc gcgggacaac gaggcccaga agctcacgtt
     2701 cgacatcctg agccagaagc tcgacctgtt cgggacggtc ctgggcgcga cggatgcggt
     2761 cctgcatcgt ccggggacgc tcgcccccga gacgctggcg agcgggctgg gcgcggagtt
     2821 cgagacccag ctgcggcgga tctacgaacg cgcccggacg ctcgaggagg tcgagcgcga
     2881 gctgcgcgag ctgcgcgatt cgatggaggc ccgtcgccgc gagttcgaga cggcgctcaa
     2941 gcgcaccgag gacgtgatcc agcgccgctt cgatgcctcc gtgagggcga cgttccgccg
     3001 catccaggcc gagtttcccc gcgagctggc cgagttcgat caccacctcg agatggtcgt
     3061 gaccgagtat ctcgatgcgg tctccgtgcc gtaccgcatg acccgtcagg acggaggcgc
     3121 catcctgaag ctcgaggacc cgtcgcgtct ccctgcctcg ctgcaggatc tcccgggctg
     3181 tgcgatcggt agctcgtcgc ccggatcgcc gtacggctcg ctgcacctgc ggcatccgct
     3241 ggtcgtcgcc gccctggagc atgcgcgcgc gtcgatccag cggaccggca gctcggtccg
     3301 ggtccgggta gccgaagccg atgatgacct ccggggtcgt cgcggtcgga tgaggctgat
     3361 ccgcgtcctt caccggaatt tcgagatcac ggagcacctg gtgccggtgg tcctgctcga
     3421 gggagacgat gccccgttgc ccgccgatgc ggcccgaaga atcctcgacg cggagatcga
     3481 ggatctgccg gccccgatcg gttgtggcgt cgcggtggag gagctcgatg acgcggtcga
     3541 tgagctcctg ttccacgaca ccagcgaggc cagccgcctc gagcagccgc gcttcgagcg
     3601 gaccctcgag cagatcgagc ggttcatcac ggaccgggtc ctcctactcg agcgacagcg
     3661 cgatgcggcg gttgcgcgcc tcaccaaggc acaggacgca cgcgatcacg cgatcggcgc
     3721 cgagcagcgc aacgcggccg agcgggcgat gcacaaggca cagaccgaga tcgactcgct
     3781 cgacgacaag ctgcgcacgc tccgcgccgg agatgacgag cgatatcgca agtggcgccg
     3841 ccacaccgaa gagcgacgct acaccccacc cgagatcgag catatcttcg acgcggagct
     3901 cgagatcgta tgaaccagcc ggaggcagac gacgtggcgc tcaagcttct gcataccgcg
     3961 gactggcacc tcggacgccg gttcccgagc ttccgggagg ccgacgagac caagctgacc
     4021 cgcgcccggg tcgacgtgct cgacaagatc ctggcggtcg ccgagcagcg cgcggtcgac
     4081 gcggtgctgt gcgccggcga cctgttcgac gagccgtcgc ccgactccga gtggtggcaa
     4141 ccggtcgcga agaagctcgc gacatggcga gcgcggattc ccatgttcct gctgcccggg
     4201 aatcacgacc cgctccagcc agggtcggtg tatcaccccg gccacgggtt ccggcacgcg
     4261 ctgccgccct gggtccgcgt ggtcgacgac gacagcttca cggcccgcct gaacgacgtc
     4321 gcggtcctgc atgcgaagcc ttgtcggtcg agcgcgggcc aggacgatcc ggcgctggcg
     4381 ctccctgcgc gcgagccggg cgatccgcgg atccggatcg gcatggtgca tggcagcacg
     4441 ttcgactacg tcgattgcca gaccaacttc ccgatctcca gggacgccgc cgcacagcgc
     4501 ggcttcgact acctcgcgat cggcgacacg cacagcttcc gcgtcgtacc gccggatgcc
     4561 aggccgccgg tcgtctatcc aggcgcaccc gaggcgacca gcttcgacga gccgggtgcc
     4621 gggaaggtcg tcgtcgtgtt cgtcagccgg tcgcggcggg tgacgctggc gccggagccg
     4681 gtcgcctact ggcaatggcg ggacgtcaag gtgacctcgc tcgccgagct ccgcgacctg
     4741 cgtgccgagc agctggcgcg caccgtcctg cgcctcaccg tggaggcccg gctgtccgcg
     4801 aacgagatgg aggaggccga ggcgatcctc cgcgagcttg gcggaaccgc cgcctcgtat
     4861 ggacgcgccg ggatcctgca gctgaaccgg tcgaagctca ccgtcgacac gcgcgggatc
     4921 gagacccagc tctccgacct cccggccgtc ctgcgcgcga cggtgacccg gctgaagcag
     4981 ctcgaggcga cggacgaccc gaaggtcgcg gagtacgcac aggcagcgct gtatcacctg
     5041 taccggctcg tgcgccagga gcgccagcca tgaggttggt caagctgtcg gtccagcggt
     5101 ttcagtgcat cgagagcgca gagctcgagc tcggtccgca cctcaacatc ctgtacggac
     5161 cgaacgacct cgggaagtcg agcctcgcct gggcgatccg cgcggtgttg ctcctccagc
     5221 acggatcggc acacgccgag cggttcagct cctggtatgc cgcggccgag ccccgtgtgg
     5281 cgctcacgtt ctgcgacgac tcgggcaggt actggcgcgt gaccaagacg ttcggcgggt
     5341 cgaccggacg gtccgagctg gagtcgtcga aggatggtca ggtcttcacc atggaggaga
     5401 aggcgcgctc ggtcgacgag aagctgcgcg tgctgctccg gtgggggctc gccacccccg
     5461 gcggcaaggg gcgaacggcc gtgcccgaga gcttcctcac ccacgtgctg ctcgcggacc
     5521 aagcacagga cagcgtgcgg aagatcctgt tcgagcccaa gctggacaag gacctcgacg
     5581 agtcagggcg cgagcgcctg gtggcggcgc tcggcgcgct cgcgcaagat ccgctgttca
     5641 agaccatcct cgaccaggcg accgagtcca tgcagcgcgc gttcaccccc aaggggcagc
     5701 gcaagcgctc ggccgggtcg ccgtggctcg aggccatcga gcggagcaag gcgctgcggg
     5761 caaagaagga ccagctcgag gcccaggtca aggagaccgc tcgcgtcgag gaacacatca
     5821 agggcctgca cgggcgccat gactccgtcg ccggcgagct gaccgaggcc gaggaggcgc
     5881 accgcgtcgc cgtccgacag ctcgaggccc agaagcagcg cgacgtgctc catggcgagg
     5941 tgcagtcctt gcagcaaagg atccgcgacg cggagaccat ggcgaagcag atcgaggtcg
     6001 catcgggcgg agccacgaag ctcgagtcgg acgccgctgc tgcgcgggag agtgcgagac
     6061 aggccgccga tcgcgccgcg caggtcgaga ccgagctcaa cgctatccgg agacagctcg
     6121 ataccgtgct gcacgatgac ccggacgccg agcggcggat ggccgagctg caggccgaag
     6181 tgggccgcac cgagtcgcag gcgcgcgaag cgctcgacgt ggtgagtgcc gccagggacg
     6241 cgttgcggac cgccggcaag ctagcggcaa atcttgtcgc ggaagacgct gccgtcaagc
     6301 agacctcggc gatcgcagat gccgccgacg ccgcggctgc ggtcgcattg cgtgacgccg
     6361 agcgcgccag gaagttcctc gcggatgccc agcagcgcca gcgcgacgct cggagccatg
     6421 atcgtgcgca ggcgcgcgag ctgcgtcgca aggagctcga gaaccaggca ctgtccctgg
     6481 cgagcaagcg cgcgcggatc gaggccacgc tccagcatgc aatgcatatc gtcgagctga
     6541 ccgcgcgtct ggtgaagacc gaggcagatg tacgggcgac gcgcgaccag ctcgcggcga
     6601 ttcacggcga gatcaccacg tacgagacag agcaccagga gctggaggcc gctcgagccg
     6661 tgctcgggca actccacgcc tacgggcaat tccgtcagct gcgcgaggag ctcgcgaggg
     6721 caacccaagc gagcgaggac gcagaccgcg agcggcggcg cgctgccgag ctccgcgaac
     6781 gcgcggagcg cctcaggtcg gaggtgcgcg gtggccttcc cgcggccgag cagatccagg
     6841 agctggatcg cttgcgccag cagcttcagg tggcggaagc agcgctcggt ggcggactct
     6901 cggtggagat ccgcccgaaa cggtcgctga cccttcgggc cacgcgcgac ggcgcgaccg
     6961 atccggcgac cacgggcagc gagctggttg cgctgaccgc gaagcgctcg atcgcgctcg
     7021 cgatcgacga ctggctggac atcgagatca cggcgggcga ggaggtcgca cggcggacgg
     7081 ccgccgagct gcgggcgcgc tggctgcgcg agggcgagtc ggtgctcgtc gagcatggcc
     7141 tcgcgtcgct cgacgagctc aggctgacgc gttcacgtag cgatgccgca gcgcgtgaca
     7201 tcgacgatgc gatacgcgag gccgagaacg ccgagcggcg cgcttcgctc gtcggcgacg
     7261 ccgacgtcgc caccgtccgc gagcagttga tgggcgcgga gagcgagctc ggtgcagccg
     7321 atcgcgagga cctcgcgacg cgcttcgccc ggctgggagc gtcctggcag accggcctgc
     7381 gcacgcgatc ggccgagctc gaagcgcggc tcgcatcgtc tcgcaccagg ctggagcaga
     7441 aacgagccgg cgtcgcgaag ctcgaggcgc agctcgacgt ccagcaaggc gaggcggcga
     7501 ggctcgaggc cgaggtcgag cgggcccagg cggagctgac cgggcccccg acggtggtcg
     7561 tcgcgacgtg cgaggccgac cagcaggcgc tcgccgtcga gcagcgggac atcgagcacc
     7621 agatcgcggg gctggccacg ggtacctccg aggaagaagc cgccgcggat gccgcggtgg
     7681 tcgctgctgg gactgcgctc cagacggctg aagcggatgc tcaggcaaag caacacacag
     7741 cacaggctgc gcgcgtcgcg caggtccggg ccagcacctg gctcgagggc atccgcacga
     7801 ccgcgaggca actcgatgcg cagggcgtat gggcatcgcg cctcgaccgc ggggatgcga
     7861 gcctgccggt cgacgcctgg gaggctgcgg tgagagacgc cgaggatcag ctcgagaagg
     7921 cgcgcgctgc acacgcacgt gcgaccgcgg cattgaccga cgcccgggcc gcccacgacg
     7981 ccgccgtgca gggcttgcgc aggaaggtgc aagacgccga ggacgcgtcg cgccaggcgc
     8041 ggagtcgcag cgacgctgcg gaagcgcttc acaaggaact catcgcgcag cacacggcgg
     8101 cggtcgtcag cctctccgag ttgaaggtga agatcgccgg tatccacgtc gacgagctcc
     8161 gggccgagat cgcgaagcgc cggacgcgca tgctcgagct cgagcccgag atgggccgtg
     8221 taaacgaggc gggtatcacc gagctggatc agaagctagg ccgcctccgc gcggagctcc
     8281 gcgagctgga cgaggagctg gcaaaggcgc gtggggcgct cgagcacgtg ggcggcgcgg
     8341 tggttcgcga gcgcctcgcc gagatcggcc aggccatcga gcaggccgag caacgcgagc
     8401 acgaggtcga ggtcgagtac gaggcatggc gactgctcca cgacacgctc cgccaggcgg
     8461 agtcgacgga gagcgcccac ctcggccgcg cgctcgcagg accggtggga tcgcggttcc
     8521 ggcagctcac gggtggccgc tacggcgaca tcgagctcgg cgcgaagctc gaggccggag
     8581 gcatccacgt tgctggtggg ctccgcgaga ttgcagcgct gtccgccgga acccaggacc
     8641 agctcgcaac cctgctccgg gtgtgcatcg cccagcagct gcggagcgcg atcatcctcg
     8701 atgatcacct gacccagagc gatccggccc gcgtggcatg gttcaacacg acgcttcgag
     8761 aagcggccga gagcgttcag atcatcctga tcacctgtcg cccgcaggag gtgcttacgg
     8821 ccgatgaggt gtgcagtccc ggacaggtgc gccgcgcgag cgcagacgga cgggtacatg
     8881 cggtcgacct ggcggcggtg atccggcgct tcgatg
//
LOCUS       DASVOR010000024         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_146365,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000024 DASVOR010000000
VERSION     DASVOR010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146365"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="VF516_00515"
     CDS             <1..1093
                     /locus_tag="VF516_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8106169.1"
                     /translation="AGLTAAVRLDALTRLVREEAAAVLALAADELADDTVLQQRGLDS
                     LMTITVRTRLAARTGLPVTMDLVFRGATPAGIAALIDGLIGPAASGAAPAADAERTRW
                     IKTLKAVAEPRARVFCFPGMGGAASGYIPLAAQLPADVELVGIQLPGREDRLGDAPIA
                     EMDLVTEEIAAAMAPLLDRPAVLFGYSFGAWLTLETSRRLEAHDRGVPLALVIATAHP
                     PTATSTDKIDKLSALLGERSSADEPIAPELIGALRGVLPDTLLGNEELLGAYLTACAA
                     DAALAENYRHAAHERVHPALDLPVLALSASSDPIVTQAQMAGWRSTTRGRFDQRSIEG
                     SHAAPIECPAATAAVILEALRGLPVTELS"
     gene            1090..1608
                     /locus_tag="VF516_00520"
     CDS             1090..1608
                     /locus_tag="VF516_00520"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX8106170.1"
                     /translation="MTTGTRHHLRGRPVALAAALLAKRAFRFAVVGGINTGLDFLAFN
                     LLLWTPLGARYPFLAAALACMAATVNSFLLNRSWTFEFRERKRGLFIQFIAVNVVGVL
                     IHAAVTYVAARLVTGVALNLAKLVAVALGCVWSYLAYSRWTFAATQPRRGDRLDPCGL
                     VADPRLAQQESP"
     gene            1689..>2800
                     /locus_tag="VF516_00525"
     CDS             1689..>2800
                     /locus_tag="VF516_00525"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8106171.1"
                     /translation="MLAPLRVEAPVYHDAVGGRYVVTRHADVKALLANRELCVDPRKA
                     NPGTYAARLLAANGGAKIELSMIDMDAPDHRRVRSLVTKAFTVSAVEALRPKTRALAE
                     QLLDAVTEPEFDFMPAFADPFPTTVIAEMLGIDPSMRDQFKVWSTITIERFDPTNTAE
                     RLAEIVAGEEGLRDLLFHEIAARRAAPRNDLIGRLAEAREGGERLTDKEIVAQCQLLL
                     VAGNTTTSDLIGNGLKNLIERPEQLAYLRAHPDVLPNAVEEMLRYDPSVDCTRRIAHT
                     DLEVAGCPIAKGEYIYLSIASANHDENLYTDPDRFDVRRPQIRHVAFGGGDHLCLGAP
                     LARLEAQEAFRAILGRFRHIGLSPRGHRYRVVPNFRG"
BASE COUNT          393 a         1048 c          950 g          409 t
ORIGIN      
        1 cgccggactc acggcggcgg ttcggctcga cgcgctgacc cggctcgtgc gagaggaggc
       61 ggcggcggtg ctcgcgctcg cggccgacga gctcgcggac gacaccgtgc tccagcagcg
      121 cgggctcgac tcgctgatga cgatcacggt gcgtacccgg ctcgccgcgc gcaccggcct
      181 cccggtgacc atggacctcg tgttccgcgg cgccacgcct gcggggatcg ccgcgctgat
      241 cgatggtctg atcgggcccg cggcatccgg cgccgcacct gccgcggacg ccgagcgtac
      301 gcgctggatc aagaccctca aggcggtcgc cgagccgcgg gcccgtgtgt tctgctttcc
      361 cggcatgggc ggcgccgcat cgggctacat cccgctcgcg gcccagctcc ccgcggacgt
      421 cgagcttgtc gggatccagc ttccgggccg cgaggatcgt ctcggcgacg cgccgatcgc
      481 cgagatggat ctcgtcaccg aggagatcgc cgcggcgatg gcgccgctgc tggatcgacc
      541 tgccgtcctg ttcggataca gcttcggcgc gtggctgacc ctcgagacca gccgccggct
      601 cgaggctcac gatcgcggcg tgccgctggc gctggtgatc gcgaccgcgc acccgccgac
      661 cgcaacctcg accgacaaga tcgacaagct gtccgcgctg ctcggcgagc gcagctcggc
      721 ggacgagccg atcgcgcccg agctgatcgg cgcgctgcgc ggcgtcctgc ccgacacctt
      781 gctcggcaac gaggagctcc tcggtgcgta cctgactgcc tgtgcggcgg acgcggcgct
      841 cgccgagaac taccggcacg cggcgcacga gcgggtccac cccgccctgg acctcccggt
      901 cctcgcgctg tcggcctcca gcgacccgat cgtgacgcag gcccagatgg ccggctggcg
      961 ctcgacgacc cgcggtcggt tcgaccagcg cagcatcgaa gggagccacg ccgccccgat
     1021 cgagtgcccg gcggcgaccg ccgcggtgat cctcgaggcg ctgcgcgggc taccggtcac
     1081 ggagctatca tgaccaccgg cacgcgccac catctccgcg gccgccccgt cgcgctcgcc
     1141 gccgcgctgc tcgcgaagcg agcgttccgg ttcgcggtcg tcggcggcat caacaccggg
     1201 ctcgacttcc tcgcgttcaa cctcctgctg tggacgccgc tcggcgcgcg ctacccgttt
     1261 ctggccgcgg cgctcgcctg catggccgcg acggtcaact cgttcctgct caatcgctcg
     1321 tggacattcg agtttcgcga gcgcaagcgc ggcctgttca tccagttcat cgcggtcaac
     1381 gtggtcggcg tgttgatcca cgccgcggtc acgtacgtcg ccgcgcggct cgtcaccggc
     1441 gtcgcgctca acctcgcgaa gctcgtcgcc gtcgcgctgg gctgcgtgtg gtcctacctc
     1501 gcgtacagcc ggtggacctt cgcggcaacg cagccgcgac gcggcgatcg cctcgacccg
     1561 tgcggcctgg ttgcagatcc gcgcctcgcg cagcaggagt ctccatgagt cagcctggcc
     1621 cgaagcgcct tccgcaaggc gaccagctct cggcgcggga tgaagcgttt cgccgcgacc
     1681 cgtatcaggt gcttgctccg ctccgcgtcg aggccccggt gtaccacgac gcggtcggcg
     1741 gccggtacgt cgtgacgcgc cacgcggacg tcaaggcgct gctggcgaac cgggagctgt
     1801 gcgtcgaccc gcgcaaggca aatcccggga cctacgcggc caggctgctc gcggccaacg
     1861 gcggggcgaa gatcgagcta tcgatgatcg acatggacgc accggaccat cgccgggtgc
     1921 ggagcctcgt cacgaaggcg ttcaccgtca gcgcagtcga ggcgctgcgg cccaagaccc
     1981 gggcgctcgc cgagcagctg ctcgacgcgg tgaccgagcc ggagttcgac ttcatgcccg
     2041 cgttcgccga tccgttcccg accacggtga tcgccgagat gctcggcatc gatccgagca
     2101 tgcgcgacca gttcaaggtg tggtcgacga tcacgatcga gcggttcgat ccgaccaaca
     2161 cggccgagcg gctcgccgag atcgtcgcgg gcgaggaagg cctgcgcgac ctgctgttcc
     2221 acgagatcgc tgcgcgccgc gcggcaccga ggaacgacct gatcggccgg ctcgccgagg
     2281 cgcgcgaggg cggcgagcgc ctcaccgaca aggagatcgt cgcccagtgc cagctgctgc
     2341 tggtcgccgg caacaccacg acctcggacc tgatcggcaa cgggctgaag aacctgatcg
     2401 agcgccccga gcagctcgcc tacctccgcg ctcacccgga cgtcctgccc aacgccgtcg
     2461 aggagatgct gcgctacgac ccctcggtcg actgcacacg ccggatcgcc cacaccgatc
     2521 tcgaggtcgc gggctgtccg atcgcaaagg gtgagtacat ctacctgtcg atcgcctcgg
     2581 cgaaccacga cgagaacttg tacacggacc cggaccggtt cgatgtccgg cgcccgcaga
     2641 tccgccacgt cgcattcggc ggcggcgacc acctctgcct cggcgcgccg ctcgcacgcc
     2701 tggaggcgca ggaggcgttt cgcgcgatcc tcggccggtt ccggcacatc ggcctgtcgc
     2761 cgcgcggcca ccgctaccgg gtggtgccga acttccgcgg
//
LOCUS       DASVOR010000025        22740 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_175606,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000025 DASVOR010000000
VERSION     DASVOR010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 22740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22740
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175606"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1144)
                     /locus_tag="VF516_00530"
     CDS             complement(212..1144)
                     /locus_tag="VF516_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106172.1"
                     /translation="MPSRTGAGLGLVHYVRFNDIPGLPWSGPTKFGGAVSYQGVSLIQ
                     SNFGSPGNLEVVAVNSSNQLVHFWRDSGPSFTWSGPLVIANGVRGVPGLIQSRFGIMG
                     NFEVVVPSLTGGLLHFWRNNDAPTLPWSGPTPFAQNLGLVDAVSLIESNFGSPGNLEL
                     AATAAAAPGFPARQVFHVFRDSATLTWSNPAPIRGDANVPAIGLPALIQSQFGTRGNF
                     ELVVNSASGLQHFWRDNDAIGFPWHGPILVAVGFQSDIPSMIESNFTPLPGLPGNLEV
                     ITETTFAVTTDLAHLWRSSATLLWSPPITVATNV"
     gene            complement(1176..1460)
                     /locus_tag="VF516_00535"
     CDS             complement(1176..1460)
                     /locus_tag="VF516_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106173.1"
                     /translation="MLFDMRVRSIAMVGRHASSVSAAPSEPNHHGWMVHTAAPGTTGS
                     NEIFDKSVAPLSRHPAHEKCTAKDPDMSPVLDAPVLPGKEVCDEWLFDSE"
     gene            complement(1845..2462)
                     /locus_tag="VF516_00540"
     CDS             complement(1845..2462)
                     /locus_tag="VF516_00540"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8106174.1"
                     /translation="MDPAQKRRATYRDVIDSPAHMTAEIIGGELRLSNRAATPATAAA
                     SALGEELGPPFKRGRGGPGGWLILDEPELHLGDDILVPDLAGWRRERLPILPDASFLT
                     VPPDWICEVLSKSTEKMDRTEKMPIYAASGVQYAWLVHPRRRSLEAYRLHDGTWAAIA
                     VYGEADRARIPPFDAIELDLAALWADVALPTRASEEPAHYAYDSP"
     gene            complement(2620..7731)
                     /locus_tag="VF516_00545"
     CDS             complement(2620..7731)
                     /locus_tag="VF516_00545"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8106175.1"
                     /translation="MGEAVITAAYRSPIPGPYPGLRAFRESEAAIFFGRDEQIDGLME
                     RLEHERLVVIAGESGCGKSSLVRAGLLPALEAGFLRGGGTQWRVADLRPGNRPIFHLA
                     QALVGVLRPGQGDDAAFLAARLRCGPRGICDLLNDFPLPSGAEILILVDQFEELIRFR
                     ARIDPDEADAFVALLLETVADKSVPVRVVLTLRSDYLGGCTIFPGLPEALNGSQYLVP
                     RLGRDELRETIINPARVFDGDVDSQLANQLINEIGQSQDQLPVLEHALLAMWNQPFNR
                     RRATDLGADHGGTPRPTHLKLDDYRSLGGLGEALSRHADVVFNSLKEDDKKQIARRLF
                     CALWDDGATGRDTRRPCSVREAAEIAGVKIDQLKEVVEAFRKAEHSFLMPPPDVPLTE
                     DTVLDVSHESLFRHWTRLKEWIRDEAEGAEHYQRLLGLARAHRQKKGDLLSSRTLQLA
                     IGWWDSQQPNVAWARKYSDRSEDFALVARFLRDSWRARRRRRWLVWGFLITVVVVGYG
                     VTFGKYRFSEEKQTGLLRQQVYSAAAFNAQRSEPVRALQLALEAQSDVDNPQADAALH
                     SALRGAHFRSILFPSLGSFQNAKFLPPDGSRIITAGEADGLAIWDVATGRRLYALHGA
                     IPATLAVDSKGARAATASDDGTVDVWDLSMRKHLWTQRHQASPITDIAFSPDGSLLAT
                     ASEAGSAVLWSVQDGEAKHRLIDHLGAVTGLAFSHDGNRIATAGADGRIVLWDTHSGH
                     KLELFTQLPDVTKLAFDATGRFLAATSGNSLMLWDPVTQRMEEPLRHTSKVTAIAFSP
                     DGRRFATTGLDGTLRVWDASSRTERFWTSSDVPLHDYGRSAGELSVSYGGEASWLRTV
                     QFSTDGRKLVTTGADGSAKVWDVEAGGESLTFRAHEQAIRRIAVSPNGDQIATGSDDG
                     TAAVWDADGHRVFGVPASRAVLALAYDATGTHLAVSDGKKVNVWTREGNRVGSLEADD
                     RVGDLQWYHGQDRIAVAAGPHVEIWSISDFRRMTVLDAKTPVQAVSISPEDDRYIASE
                     CRDPEDADESAHHICLWDAHSFHKIAVLEPHAHGTRGDGQPQGVKEAGRGRAILSLCF
                     SRDGSWLASSDDKTIKIWEIGGLGGHLRATLVGHREGIAGLAISPDGRLLVSSGADRI
                     RLWNATSYLPQDGFPDAEFSSSSVAFYRDGRRFVAGGNDGVVRVYRIDSTSLQALAED
                     RVPVPLPDSECKRNTNTDCHSRATSLQLTNEIRYLLHRGPPTTWGQSIAMAVRIDPWQ
                     FGVESTRFANARNIREAVDAASIVLQRVSNNASHAPASVHGKASEEFAELLASASHTG
                     QIRSEALLEQVQRDATVLLDATELTSRIETLLLTGLARNYAEDGKPALAIKALELVPV
                     SHDGGRWAEHAVTDIANAAVMRVVRDQLTREEYKAAAEKVRALSISTEEHSDRTVVLQ
                     RFVATTLASSGNYPGAIKATASLAFREGGDVELLAEHADILRYAGYPDRALAMLARGL
                     NIDPTNDRMLWLQAQVQSQLGLFDDAAATLRKISPLAPFYMDAAAMAGVISYAKLARL
                     TEGFRSMVLTVDPQDASSWAKLAQAAWATGRLREAWLIAGRVLEARAVKQSSPDVELA
                     MHFVRVAALCQSQDWDHAEAELGALLATAPLVTTRTWSYEGNRASLGHIKEPKIQQFV
                     RELLAYSESRGQTGTPQQLIQLLHEARDAPPVPTH"
     gene            complement(7724..8902)
                     /locus_tag="VF516_00550"
     CDS             complement(7724..8902)
                     /locus_tag="VF516_00550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106176.1"
                     /translation="MQNAVAVVLVLSPAFLMSDWWTEPVLLNALRSVARNRPDHVIIV
                     EKTEVPHGASMELDPHRRSVFWRSDRGVPRTLGLPSVAAEPDKRTYFALVSDLATALA
                     AHLKPRIEIPEQRPSVPGEPDEHRTVFLAECSKDVEDRRLHVLRYLEQAGFKVVPRGQ
                     LPSDPDQFETEATRLIVSARLFVQILGATADRPARAPRAVYRQLDIAMSQHREILQWR
                     DPSLDLANVADLELQRVLLRDTVHADSIPAFCSAVRAAASRAPAPAAGPKPSVFVDAS
                     RNEVPTLGKVFGERYEHIQWDWHEPKRTELKRILRVVDGVVLYWGHEGSERTQTRYYL
                     FQSYFKALKKSTKRLLIYDGPPEDKPDFQGAGTWPLARARDGREPVEFRDFLQEIAHG
                     "
     gene            complement(9081..9704)
                     /locus_tag="VF516_00555"
     CDS             complement(9081..9704)
                     /locus_tag="VF516_00555"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8106177.1"
                     /translation="MAYVPGWQNDVFVSYAHVDDQPLPGARDGWVSLLGDALKTFLAM
                     QLGRAEVLAVWRDRQLTGHTQLTPEILTAARNSATLLIVLSEGYLASDWCWSEYREFV
                     KLAGAGTRRLFVVERMPIERGRKPEELRDLLGYPFWVRDHSDGPARTLGVPVPRPDEP
                     EYYHRLNRLALELAGELRRIKLQREEQHHLNIRQDGMESINDQSIRT"
     gene            complement(10028..10966)
                     /locus_tag="VF516_00560"
     CDS             complement(10028..10966)
                     /locus_tag="VF516_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106178.1"
                     /translation="MFWRADRGANRTLGFPSVAAEPDKQKYFALVSDLATALAAHLKP
                     RSDPGASAPVADEPDEQRTVFLAECTKDVEDRRLHVLRYLEQAGFKVVPRGQLPSDRD
                     QFEAEACRLIAGARVFVQILGATADRPGRVPRAVYRQLDIAMTQHRDILQWRDPSLDL
                     ANVTDLELQRVLLRDTVHADSIPAFCSAVRAAASRTPAPAAAPMPSVFVDASRSEVAR
                     LSQIFGRYRHIHWDWHEPKRTELKRILQVVDGVVLYWGPESSERIQTRYYLFQSYFKA
                     LKKSTKRLLIYDGPPRGQAGLPGRGNLAARLGPRWP"
     gene            11007..11657
                     /locus_tag="VF516_00565"
     CDS             11007..11657
                     /locus_tag="VF516_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106179.1"
                     /translation="MTAVRSTTINRSGRSRLTVWLILALADLVDQLIGEHRVDVAIEH
                     ARRVDDRFAQLLALQARHQVLAAIERLGKTGEDRAAPEIVGPQRRDDAHRQGWVRNGV
                     EQQRHERVRFAGIRTSTEPDQLFELVDDDQQLRVRWQRQVVEDLGKAARAGAQPCGEQ
                     RGRVASRCGVEHGAQRLRKAVDRLAARPERGGSPPGATAAKKAGLQRRQQAGAHER"
     gene            complement(12139..17274)
                     /locus_tag="VF516_00570"
     CDS             complement(12139..17274)
                     /locus_tag="VF516_00570"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8106180.1"
                     /translation="MADGARLASASRVALSPYPGLRAFRQDEAAIFFGRDEQIDRLLD
                     RLERERLVAVAGESGCGKSSLVRAGLLPAVEAGLLHGGGPRWRIVDLRPGNRPIHHLA
                     QKLAHVLSSDPAKDAPLLAARLHYGPRSLADILRDFQLPDGTELLILVDQFEELIRFR
                     ARIDPDEADAFVALLLETVADETIPVRVVLTLRTDYLGGCASFAGLPEALNGSQYLVP
                     RLTREQLREAIVNPARVFEGDVDSTLTNQLINEIGLRQDQLPVLEHALLAMWRQARDR
                     AAQRAPEAAGPIKLTIDDYAALGGIGEALSRHADEVYRALGSAEQQRIASRLFCALWD
                     GNASGSDTRRPCTVREAAEIAGVSVEAMIAVADAFRRSEHSLLMPPPEIALAEDTVLD
                     VGHESLFRHWKRLDEWIAKEADDAKQYRRWLHEASARDHGNGDLLSDKAMDHARTWQA
                     ALQPTEAWARRYSEHPEDFANAQRFLRDSLRSQRIRQWRNRSVIVLAAGLFFAGVYVL
                     ARNSEQREQERRHAELQRQQIYSAAAFGARLEPVRALQLALEAQGDDPDPQAGTALHG
                     ALRASHFRSMVFPSLGSFDDARFLDDQQIVTVGVGDGLAVWDIATGRRLYALYGAIPH
                     RLAIYTAQRTALAATASDDGTVDLWDLSGRTQLWTGRRQVMAVNDIAFSPDGRWLATA
                     SDDGSAVLWDVEHGKPRPPLIDHLGAVIALAFSHDSQRLATVGSDGRIVLWDTERGKK
                     LELFARSPGTSRITFDASGRYVAAAGSYGVTLWDAITHHEEGSLLQSREITAVAFSPD
                     SPRIATTGLDGTLRVLDARDRRQRFSTPADVPLHSDGRPATELAASRDDEASWLQTVA
                     FRADGRALVTTGRDGSAKIWDVEAGGELLTFRAAEGAIRRIAYHPAGDRIVTAGDDGA
                     VSLWSREGRLVAQLRGDDVVRAMDLDARGTRLAFARGHHVGFWDLASPAPSEPALELP
                     DDVQDLQMFHTADRLVVAAGHKLAVWHTSRNGPDFTIDTGTQVHAVAISPRDDVIASE
                     CGIAIDAEPEARPICLWKASSGKLSTILGAPAPGEDRPSEDGEAHRKPILALRFSSDG
                     QWLASSSEDKTIKIWKIDARSHLATLRGHHESITGLALTPDGKLLASSGNDLVRLWNT
                     SSHLPQEGFPDAEFGSDSVAFSPDGKSFVAGGHDGVVRFYAVDSDALRLLARDRTPIY
                     LTESECVRYANQGCREPRTPLQRANEGRHMLQHGFSLYGDYSLAIAAYQDPTAFEQEF
                     ARFRSAQIIHHAIDGITGRLFRVAKNVASRTSLLEDGSERFGKELAAARLRTVQLGEP
                     CLQHARRSDANIVLDPAALASRIAALQLSEQARQFAVAGAQPSAEHAFRLTPPASDEA
                     HWETHAAAITANLAAVKRVQDHMASGERHAAIEGLKGIVEELQRADRMHQPVGELQRV
                     DRLHQQVLLRFIASRSEATDMNDVVTPWIERVYRQIDDVEALAVLADFLRHTQKRDKA
                     RLVLDHALDLEPTNDLALQYFGEIQRDFGQPARALNALRAISPMAPDYRKTARVAGEI
                     ANEQLELLKEGYRWMSLAVDPTDPVGWANLAQTAWANNRFIQARLLASRVLEARAIQR
                     STPDVELAMRFVLVAALYQSQDLAAAEPALEALVTLAPQVTRQTWSYNGSRAAVGRIK
                     EASTKGFLGALLRYCETHGQVGNPDDLLAWLRAARAAKGAVPR"
     gene            complement(17267..18604)
                     /locus_tag="VF516_00575"
     CDS             complement(17267..18604)
                     /locus_tag="VF516_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106181.1"
                     /translation="MPTDVFFCFADEDNATFGNQPNGWVDDLVDCVTKLLGGRGIAEP
                     RVLSKSPGHFPAAERAELVRRSATIVVVLSPAFFNSDWVRSEALAEAIRSVATAAPQR
                     VVIVEKSEVPRQIAAEFDAHRRAVFWRSVHGTPRTLGFPSLAAEPDQQAYFAVVFDLI
                     LALASQLSPAGAAASAAAGPDTGARQRVFLAEATRDVEARRLEVLRYLEQAGFEVVPH
                     GRLPSDPAQFEAETARLIDSVRLFVQLLGTAPEAPDQVPGAVARQLEIAVRKGREIQQ
                     WRDPSTDVMRLQDPRLRELLLRDTVQASSIPAFCQAVCAALRAQTTLPSVFVDASRDQ
                     VAQLEEIFGRYDNIRWAWHQPKDSELRTVLRAVNGVVLYWGAESSERPLSRYYKFEGL
                     FKALKKNLKRLRIYDGPPENKPELLGAHWPMIRGRDGGEPQEFREFLQEIANG"
     gene            complement(18630..19220)
                     /locus_tag="VF516_00580"
     CDS             complement(18630..19220)
                     /locus_tag="VF516_00580"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8106182.1"
                     /translation="MTYVPGFQNDVFVSYAHVDDQPLPGARDGWVSLLGDALKILLAE
                     QLGRAEVLAVWRDFQLTGHAELTPEIFGALRSSATLLIVLSEGYLASDWCWSEYREFV
                     QLAGPGTRRLFVVERMPIERDRKPEQLRDLLGYPFWVRDRRDRAPRVLGVPVPRPDEP
                     EYYHRLHGLALELASELKRMAAAAPPQASDPITHRP"
     gene            complement(19421..20161)
                     /locus_tag="VF516_00585"
     CDS             complement(19421..20161)
                     /locus_tag="VF516_00585"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8106183.1"
                     /translation="MPATIDITTEDGTCPAHVFQPSGAGPWPAVLFFMDGLGIRPALF
                     EMGARLAAHGYYVILPDLYYRSGPYEPMDPRRVFGDPEVRQTLFQKFMGKLGQANAMR
                     DTRAVLAFLAGQPAVAPGQIGATGYCMGGGLAISAAGFFPDRFAAVAAYHPGHLATDA
                     PDSPHLQAPRIQAQVYVGGASDDPSFPDAMKARLETALRDAGVRHTVETYPARHGWVP
                     SDTPVHDPAAAERHWQTLFALLDGALKP"
     gene            complement(20247..21599)
                     /locus_tag="VF516_00590"
     CDS             complement(20247..21599)
                     /locus_tag="VF516_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEX8106184.1"
                     /translation="MNQPNQKLTRTDAAWLDAHWMPFTGNREFKAHPRMLVAAEGAYY
                     TDADGRKILDGLSGLWCTGLGHGRREITEAISRQAATLDYAPAFQYGHPQSFALANKI
                     KELTPDGLDHVFFTCSGSETVDTSLKIARAYWRARGQGTKTRLIGRERGYHGVNFGGI
                     SVGGIGANRKIFGQGIEADHLPHTQVAVYDKADAFTRGMPRRGAELADDLLRLIALHD
                     ASNIAAVIVEPFAGSAGVIIPPAGYLQRLREICTANNILLIFDEVITGFGRCGAYTGA
                     AAFGVTPDLLTCAKQITNGTQPLGAVVVKKEIYDAFMATGGPEYMVELAHGYTYSAHP
                     IACAAGVAALDLLVQEKAIERVAALAPHFENAVHSLKGAKHVADIRNFGLAAGLTLAP
                     VPGEPARRPFEVAMKCFAAGVYVRYGGDTIQLAPPFVIEKSEIDRLVSVLGDALDKTA
                     "
     gene            21891..>22740
                     /locus_tag="VF516_00595"
     CDS             21891..>22740
                     /locus_tag="VF516_00595"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8106185.1"
                     /translation="MANRYRSSALVGALILSTAGRTWADRSITLEDALALARTHNRDL
                     RSARERVVEAEAGVAQARAALLPTVSAQGKYTRNYKEVDFDAIAFSQPTFDVANAIRT
                     STTNPAEAAAIQGVVDRDRALLAGQPTVVIQKLNQLDGVVSAAVPLVSPPAYYGLSAA
                     QSSRDASAAGYHVTEAGVLLSVAQAYFAAAGTDELVLARQDAVKVATETFDVARARVA
                     AELANQVESTRAETALVRAQQDLAEAENTRGAAYRALATLIGTHEPIAVQATRTPPEE
                     PGPFEAL"
BASE COUNT         3470 a         7682 c         7923 g         3665 t
ORIGIN      
        1 gcgggcgcgg ctccgacacc catccgcgca gcagcccccg gcgagtcgcg tgtaggcgct
       61 ctcccgggcg ctaggcgcta cacgctcctc gcacgtgctg tcgcgcggcg agcggatcgt
      121 gcagaacgat ccgtgggtcg gcaaacagaa gcagggccgc ggccagttcc tccgccgcgg
      181 cacgttcggg cggtcgcgga tgcccgctgg ctcaaacgtt ggtcgcgacg gtgatcgggg
      241 ggctccacag cagggtcgcc gagctcctcc agaggtgagc gagatcggtc gtcacggcga
      301 acgtggtttc cgtgatgacc tccaggttcc cgggtaatcc agggagtgga gtgaagttgc
      361 tctcgatcat ggaaggaata tcggactgga aaccgacagc gacgagaatg gggccatgcc
      421 agggaaagcc gatcgcgtca ttgtcacgcc agaaatgctg caggccactc gcgctgttga
      481 cgacgagctc gaagttcccg cgcgtcccga actggctctg gatgagcgcc gggagaccga
      541 tcgccgggac attggcatcg ccccggatcg gggcgggatt gctccacgtc aacgtcgcgg
      601 aatctcggaa gacgtggaac acctgcctgg ccgggaaacc aggggcggcg gcagccgtgg
      661 cagcgagctc caggttcccg gggctgccga agttgctctc gatcaggctg accgcgtcga
      721 ccagcccgag gttctgcgca aacggggtag gcccgctcca cggcagggtg ggggcgtcgt
      781 tgttacgcca gaaatggagc agcccaccgg tcaacgaggg gacgacgacc tcgaagttcc
      841 ccatgattcc aaaccggctc tgaatgagcc cgggaacccc gcggaccccg ttcgcgatga
      901 cgagcggccc gctccacgtg aacgacggcc ccgagtcccg ccagaagtgg accagctggt
      961 ttgaactgtt gacggcgacc acctcgaggt tcccggggct gccgaagttg ctctggatca
     1021 ggctcactcc ctggtacgac actgcccccc cgaacttcgt agggccgctc cacggcaagc
     1081 cagggatgtc gttgaaccgg acgtagtgca cgagcccgag cccagcgccc gtcctgctcg
     1141 gcacgacaac ctcgaagttg cctgtggttc caaacttact ctgaatcaaa gagccactca
     1201 tcacacactt cctttcccgg caataccggc gcatcgagca ccgggctcat atccgggtcc
     1261 ttggcggtgc atttctcatg cgcggggtgg cggctcaggg gcgcgacgga tttgtcgaat
     1321 atttcgttgc tgcctgtcgt gcctggggcc gccgtatgaa ccatccagcc gtggtggttt
     1381 ggctcagagg gcgctgccga cacggagctc gcgtggcgcc ccaccatggc gatcgaccga
     1441 actctcatgt cgaacagcac accggataga gatcgtggtc gtctatacct tttaaaccga
     1501 tgtgacgatc aggccgccct gctccaacat gaaatctctc cgcacggcgt gcgacgggaa
     1561 actgtccggg tgggtgtggc gagtgtccga cgatggcgcc gtgcccggga gcttgtaggc
     1621 cgaagaccgc gagcgccgat gcgcggggcg gtcccgcgcg cggctgccac gctgcgggcc
     1681 cccggtgacc cagagcctcg gcgcgcacac gcacggtcgg gtgctgtcgc gcggcgagct
     1741 gatcgtgcgg aacgacgagg cgtggctaat tgccgcgcag gcggccctgg agcggctttg
     1801 ccgcggatca gctcgccaca gcaggttggg cgtgagggct cggctcaggg gctgtcgtac
     1861 gcgtagtgcg cgggctcctc cgaggcgcgg gtcggcagcg cgacgtccgc ccacagcgcg
     1921 gcgaggtcga gctcgatcgc gtcgaacggc gggatccgtg cgcggtcggc ctcgccgtag
     1981 acggcgatcg cggcccaggt tccgtcgtga agccggtagg cttccagcga tcgccggcgt
     2041 ggatggacga gccaggcgta ctggacgccg gacgcggcgt agatcggcat cttctccgtg
     2101 cggtccatct tctcggtcga cttcgagagc acctcgcaga tccagtccgg cggtactgtg
     2161 aggaaggagg catcgggcag gatcggcagc cgctcgcgcc gccagcccgc gagatcgggg
     2221 acgaggatgt cgtcgccgag gtggagctcg ggttcatcga ggatcaacca gccccctggg
     2281 cccccacgac cgcgcttgaa cggcggaccg agctcctcgc caagggccga agccgcggcg
     2341 gtggcagggg tggcggctcg gttcgacagc cgcagctcgc cgccgatgat ctcagcggtc
     2401 atgtgcgcgg gcgagtcgat cacgtctcga taggtcgcgc gtcgcttctg ggcggggtcc
     2461 attccgagaa cctaccaggc gagtctgaca atgaatccgt cgatggcgtg ccagtgacgt
     2521 gatcgacgac gatcatggaa gcatccgcat tgccggccgc tgcgatgctg cgcagcctgc
     2581 acggcgcagg gggcctggca gggatcgatg gggcagagct caatgcgttg gcaccggcgg
     2641 cgcgtctcgt gcctcgtgga gcagctggat gagttgttgt ggggtcccgg tctggccccg
     2701 gctctcgctg taggcgagga gctcccggac gaactgctga atcttgggtt ccttgatgtg
     2761 ccccagcgag gcgcgattcc cctcgtagct ccaggtcctg gtggtcacca gcggcgccgt
     2821 cgcgagcagc gcgccgagct ccgcctcggc gtggtcccag tcctgggact ggcacagcgc
     2881 tgcgacccgg acgaagtgca tcgcgagctc gacatccggc gatgattgct tcaccgcgcg
     2941 tgcctccagc acgcgaccgg cgatcagcca ggcctcgcga agccgaccgg tcgcccacgc
     3001 agcctgcgcg agcttggccc agctgctcgc gtcctgcgga tcgacggtaa ggaccatgga
     3061 gcgaaaccct tcggtcagtc gcgccagctt ggcatacgag atcacgcccg ccatggccgc
     3121 ggcatccatg tagaacgggg ccagcggaga tatcttgcgc aacgtcgccg ccgcgtcatc
     3181 gaacagcccg agctgggact gcacctgggc ctgaagccat agcatacgat cattcgtggg
     3241 atcgatgttc aggcctcgcg ccagcatcgc gagcgctctg tcggggtagc cggcatagcg
     3301 cagaatatcc gcatgctcgg caagcaattc gacgtcgccg ccctctcgga aagcgagtga
     3361 cgcggtcgcc ttgatggccc cggggtagtt ccccgagctc gcgagcgtcg tcgcgacaaa
     3421 acgctgcagc acgaccgtcc ggtcactgtg ttcctccgtg gagatcgaga gcgcgcgaac
     3481 tttctcggca gccgccttgt attcttctcg agtgagctga tctctcacca cacgcatcac
     3541 cgcggcgttg gcgatatccg tgactgcgtg ctctgcccag cgacccccgt cgtgggagac
     3601 gggcacgagt tccagggcct tgatcgccag cgctggcttc ccgtcctcgg cgtagttcct
     3661 ggccagacct gtcaagagca gcgtctcgat cctcgacgtc agctcggtcg catcgagcag
     3721 gaccgtggca tctcgctgga cttgctcgag caatgcctcg ctccggatct gcccggtgtg
     3781 cgatgccgat gccagcagct cggcaaattc ttcgctggcc ttgccgtgaa cgctcgccgg
     3841 tgcgtggctt gcgttgttac tcaccctctg cagcacgatg gaggcggcat ccacggcctc
     3901 tcgaatgttc cgggcattcg caaaccgcgt actttcgacc ccgaactgcc acggatcgat
     3961 gcgcacggcc atggcgatgc tctgccccca ggtggtggga ggtccgcggt gcagcaggta
     4021 cctgatctcg ttggtgagtt gaagactcgt ggcccggctg tggcagtccg tgttggtatt
     4081 tcgtttgcat tcgctatccg gcagaggaac cgggacgcga tcttctgcaa gcgcctgtag
     4141 tgaagtgctg tctatccgat acactcgaac gacgccatcg ttgccccccg cgacgaacct
     4201 cctgccatct cgatagaagg caacgctgct cgagctgaac tctgcatcag gaaatccgtc
     4261 ttgcggaagg taggaagttg cgttccacag ccggatccga tcagcgccgc tcgagaccag
     4321 cagtcggccg tcggggctga tcgccaggcc ggcgatgccc tcgcggtggc cgacgagcgt
     4381 cgctcggaga tggccgccaa ggcctccgat ctcccagatc ttgatggtct tgtcgtcgct
     4441 cgatgcaagc caggatccgt cccggctgaa gcacaaggac aggatggctc gaccgcgccc
     4501 ggcctccttc acgccttgcg gctggccatc gccgcgcgtg ccatgggcat gcggctccag
     4561 caccgcgatc ttgtggaagc tgtgcgcgtc ccacaggcag atgtggtgcg cggattcgtc
     4621 ggcgtcttca ggatcccggc actcgctggc aatgtacctg tcatcctctg gactgatgga
     4681 caccgcctgg accggcgtct tcgcatccag cacggtcatg cgccggaagt cagagatgct
     4741 ccagatctcg acgtgcggac cggcagcgac ggcgatgcga tcctggccat gataccattg
     4801 aagatcgccg actcgatcgt cggcctccag ggagccgaca cggttgccct cgcgggtcca
     4861 tacgttgacc ttcttgccat ctgacaccgc gaggtgcgtt ccggtcgcgt cgtatgccag
     4921 tgccagcacg gccctagatg ctggcacacc gaagactcgg tgaccgtccg catcccacac
     4981 agccgccgta ccgtcatcgc ttcctgtagc aatctggtcg ccattcgggc tcaccgcgat
     5041 gcggcggatc gcttgttcgt gcgctcggaa cgtcaaggac tcaccaccgg cctcgacatc
     5101 ccagaccttc gcactgccat cggcgcccgt ggtcaccagc ttgcggccat ccgtgctgaa
     5161 ctggacggtc cgcagccagg acgcttcacc cccataggac acggacagct ctccggccga
     5221 gcggccatag tcatgcagtg gcacgtccga cgacgtccag aaccgctcgg tgcggctgct
     5281 tgcatcccag acccggagcg taccgtcgag gccggtggtg gcgaaccgcc tgccgtcggg
     5341 actgaacgcg atcgccgtga ccttgctggt atggcgcagc ggttcttcca tgcgctgcgt
     5401 caccgggtcc cacagcatca ggctgttgcc cgacgtcgcc gccaggaagc ggccggtcgc
     5461 atcgaaggcg agcttggtaa cgtcagggag ctgcgtgaac agctcgagct tgtggccaga
     5521 gtgcgtgtcc cacagcacga tgcggccatc cgcgccagcc gttgcgatgc ggttgccgtc
     5581 atggctgaac gcgagcccgg tcaccgctcc gaggtgatcg atcaagcggt gctttgcctc
     5641 gccatcctgc acgctccaca ggaccgcgct gcctgcctcg ctcgcggtcg caagcaggga
     5701 gccgtcaggg ctgaacgcga tatctgtaat gggcgaggcc tggtgtcgtt gggtccacag
     5761 atgcttcctc atcgagagat cccatacatc cacggtgcca tcgtcgctcg cagtggccgc
     5821 gcgcgctccc ttcgagtcaa ccgccagcgt ggccgggatc gcaccatgga gcgcgtacag
     5881 tcgacgccct gtcgcgacgt cccatattgc gagaccgtct gcttcaccgg cggtgatgat
     5941 gcggcttccg tccggcggca ggaactttgc attctggaac gagcccagcg atggaaacag
     6001 gatgctccga aaatgcgcgc cacgaagcgc actgtgcagc gcggcatcgg cctgtggatt
     6061 atcgacatca ctttgcgcct ccagcgcaag ttgaagcgcg cgcacaggct ccgagcgctg
     6121 tgcgttgaat gccgccgcac tgtagacctg ctgtcgcagc agtcccgtct gtttttcctc
     6181 cgagaatctg tacttgccga aggtgacgcc gtaccccacc accaccaccg tgatcaagaa
     6241 tccccagacc agccacctgc gtcttctcct tgccctccag ctgtcccgca ggaagcgcgc
     6301 gaccagcgcg aagtcttcgg aacgatccga gtacttgcgt gcccacgcga cgttcggctg
     6361 ctgcgagtcc caccagccga tggcgagctg aagcgtcctg ctgctcagca gatcgccctt
     6421 cttctgccgg tgggctcgcg cgagccccag caggcgctgg tagtgttcgg cgccttcggc
     6481 ttcgtcgcgg atccactcct tcagccgggt ccagtgccgg aacaggctct cgtggctgac
     6541 atccagcacg gtgtcttcgg tgagcgggac atccggcggt ggcatcaaga acgaatgctc
     6601 ggccttgcgg aacgcctcca cgacctcctt gagctggtcg atcttcaccc cggcgatctc
     6661 ggcggcctcg cgaaccgagc acggtcgccg cgtgtcgcgg ccggtggcgc cgtcgtccca
     6721 cagcgcgcag aacaggcggc gcgcgatctg cttcttgtcg tcctccttga gcgagttgaa
     6781 gaccacatcg gcgtgccggg acagcgcctc gccgagccca cccagcgacc ggtagtcatc
     6841 gagcttgaga tgggtcggcc gcggcgtgcc tccgtggtcg gcgccgagat cggtggccct
     6901 ccggcggttg aacggctggt tccacatcgc cagcagcgca tgctccagga cgggcagctg
     6961 atcctggctc tgaccgatct cgttgatcag ctggtttgcg agctgcgagt cgacgtcgcc
     7021 gtcgaatacg cgcgccgggt tgatgatggt ctcgcgcagc tcgtcgcgtc cgaggcgggg
     7081 gaccaggtac tggctgccgt tcagggcttc cggaaggccg gggaagatcg tacagccccc
     7141 gaggtaatcg ctgcgcaggg tcaagaccac gcgaaccggc accgacttgt cggcgacggt
     7201 ctcgagcagc agcgcgacga aggcgtccgc ctcgtccgga tcgatgcggg cgcggaaccg
     7261 gatcagctcc tcgaattgat cgaccaggat caggatctcg gcgcccgacg gcagcgggaa
     7321 gtcattcagc agatcgcaga tgccgcgagg cccgcagcga agtcgcgcgg cgaggaacgc
     7381 ggcgtcatcg ccctgaccgg ggcgaagcac gccgaccaga gcctgtgcga gatgaaagat
     7441 cgggcggttc cccggcctga gatccgcgac tctccactgc gtgccgccgc cgcgcaggaa
     7501 cccggcctcc agcgctggca gcaagcccgc gcgtacgagc gatgacttgc cgcaacccga
     7561 ctcgccggcg atcacgacca gccgctcgtg ctccaggcgt tccatgagcc cgtcgatctg
     7621 ctcatcgcga ccgaagaaga tcgcggcctc gctctcgcgg aacgcccgga gcccggggta
     7681 gggcccgggg atcggcgagc ggtacgccgc cgtgatcacc gcttcaccca tgggcgatct
     7741 cctgcaggaa gtcccggaac tcgacgggct cacggccatc gcgggcccgg gcgagcggcc
     7801 aggtccccgc gccctggaag tccggcttgt cctcgggggg gccgtcgtag atcagcagtc
     7861 gcttcgtgct cttcttgagc gccttgaagt agctctgaaa caggtagtag cgggtctgtg
     7921 tgcgctcgct gccctcgtgc ccccagtaca gcacgacgcc atccacgacc cgcaagatgc
     7981 gcttcagctc ggtccgcttc ggttcatgcc agtcccactg gatgtgttcg tagcgctctc
     8041 cgaagacctt cccgagggtg ggcacctcat tgcggctcgc gtccacgaac acgctgggct
     8101 tcggtccggc agcgggtgcc ggtgcgcgcg atgccgcggc gcgcaccgcg ctgcagaacg
     8161 ccgggatgct atcggcgtga accgtgtcac gcagcaggac gcgctggagc tcgaggtccg
     8221 ccacgttcgc cagatcgagg ctcgggtcgc gccactggag gatctcccgg tgctgactca
     8281 tcgcgatgtc gagctggcgg tacaccgcgc ggggagctcg ggccggccga tccgccgtcg
     8341 caccgaggat ctggacgaac aaccgggcgc tcacgatgag tcgagtcgcc tccgtttcga
     8401 actggtctgg atccgagggc agctggccgc gagggacgac cttgaatccc gcctgttcga
     8461 ggtagcgcag gacatggagc cgccgatcct cgacgtcctt cgagcactcg gcgaggaaca
     8521 cggttcgatg ttcgtcgggc tcgcctggga ccgatggtct ctgctccggg atctcgatgc
     8581 gcggcttcag atgagccgcc agcgcggtgg cgaggtccga cacgagcgcg aagtaggtcc
     8641 gcttgtccgg ctcggccgcg acgctgggaa gcccgagcgt gcggggtacc ccacgatcgc
     8701 tgcgccagaa cacgctccga cggtgaggat cgagctccat ggatgcgccg tgcgggacct
     8761 cggtcttctc gacgatgatc acgtgatcag ggcggttgcg tgcgacgctg cggagcgcat
     8821 tcaggagcac cggctcggtc caccagtcgg acatcaagaa tgccggagac agcaccagca
     8881 ccacggcgac cgcgttctgc acgcctcggc tccgttcggc gagcgtctcg ttgcctgccg
     8941 tgcgtgcttt tgtgcgtgcg ttgatgaccc cgctcatgcc cagggacttc cggacgctgt
     9001 ctaccagctc atcgatccag cctccgtcac cgccgcccgg cagtacgaca ttgtcctctt
     9061 cggcaaagca gaagaataca tcatgttcgt atgctctgat cattgatact ctccataccg
     9121 tcttggcgta tgttgaggtg gtgctgttcc tcgcgttgca gcttgatccg gcggagctcg
     9181 ccggcgagct ccagggcaag gcggttgaga cggtggtaat actccggctc gtccggcctc
     9241 gggaccggca cgccgagcgt gcgcgctggc ccatcgctgt ggtcacggac ccagaatgga
     9301 tagccgagca ggtcgcgtag ctcctccggc ttccggccgc gctcgatcgg catgcgctcg
     9361 accacgaaca ggcgccgggt gccggcgccg gccagcttga cgaactcgcg gtactcgctc
     9421 cagcaccagt ccgaggccag gtatccctcg gacaggacga tgagcaatgt cgcggagttg
     9481 cgcgccgcgg tcaggatctc gggcgtcagc tgtgtgtggc cggtcagctg ccggtcgcgc
     9541 cacacggcaa gcacctcggc gcggcccagt tgcatcgcga ggaaggtctt cagggcatcg
     9601 cccagcaggc tgacccaacc atcccgcgcc cctggaagcg gctggtcatc gacgtgggca
     9661 tagctgacga agacgtcgtt ctgccaacca ggtacgtacg ccatcgctac tccctcacca
     9721 ccgcgatcgc tcgtccgccg tgcgcgcttg tagcgcggca tcgagcggac gcgatgcagc
     9781 ccatgcggtg cccccgtgac atcggtccat gaggcgatcc gtcctggcaa accacgacag
     9841 cctcatggtg aggagcaccg atcccagcgc cggctggggc gttctgctgc ggagccgctc
     9901 gccgcggcac gtccgggagt catgagctgg tcgatcagct ggttggggat cggcgagcgg
     9961 tgcgccgccg tgatcaccgg ttcacccatg ggcgatctcc tgcaggaagc cccggaactc
    10021 gacgggctca cggccatcgc gggcccagac gagcggccag gttcccgcgc cctggaaggc
    10081 cggcttgtcc tcgggggggg ccgtcgtaga tcagcaggcg cttcgtgctc ttcttgagcg
    10141 ccttgaagta gctctgaaac aggtagtagc gggtctgtat gcgctcgctg ctctcgggcc
    10201 cccagtacag cacgacgcca tccacgacct ggaggatgcg cttcagctcg gtgcgcttcg
    10261 gttcatgcca gtcccaatgg atatgcctgt agcgtccgaa gatctggctg agcctggcaa
    10321 cctcgctgcg gctcgcgtcg acgaacacgc tcggcatcgg tgcggcagca ggtgccggtg
    10381 tgcgcgatgc cgcggcgcgc accgcgctgc agaacgccgg gatgctgtcg gcgtgaaccg
    10441 tgtcgcgcag caggacgcgc tggagctcga ggtccgtcac gttcgccaga tcgaggctcg
    10501 ggtcgcgcca ctgaaggatg tcccggtgct gagtcatcgc gatgtcgagc tggcggtaca
    10561 ccgcgcgggg aactcggccc ggccgatctg ccgtcgcgcc gaggatctgg acgaacaccc
    10621 gggcgcccgc gatcagccga caggcctcgg cctcgaactg gtcgcggtcc gagggcagct
    10681 ggccgcgagg gacgaccttg aagcctgcct gctcgaggta gcgcaggaca tggagccgcc
    10741 gatcctcgac gtccttcgtg cactcggcga ggaacacggt gcgctgttcg tcgggctcgt
    10801 ctgccacggg cgcggacgcg ccgggatcgc tgcgcggctt gaggtgagcg gccagcgcgg
    10861 tggcgagatc cgacacgagc gcgaagtact tctgcttgtc cggctcggcc gcgacactgg
    10921 ggaacccgag cgtgcgattc gcgccgcgat cggcgcgcca gaacacgcag cggcggtgga
    10981 catcgaagcg agtggtctgt tctcgcgtga ccgcagtgcg ctcgacgacg atcaatcgct
    11041 cgggccgctc gcggctcacg gtctggctga tcctggcgct ggccgatctc gttgatcagc
    11101 tgattggcga gcaccgggtc gacgtcgcca tcgaacacgc gcgccgggtt gacgatcgct
    11161 tcgcgcagct gctcgcgctc caggcgaggc accaggtact ggctgccatt gagcgcctcg
    11221 ggaaaaccgg ggaagatcgt gcagctcccg agatagtcgg tccgcagcgt cgcgacgacg
    11281 cgcaccggca gggatgggtc cgcaacggtg tcgagcagca gcgccacgaa cgcgtccgct
    11341 tcgctggcat ccgcacgagc acggaaccgg atcagctctt cgaactggtc gacgacgatc
    11401 agcagctccg tgtccggtgg cagcggcagg tcgtggagga tctcggcaag gccgcgcggg
    11461 ctggcgcgca gccgtgcggc gagcagcgcg gtcgcgtcgc ttcccgatgc ggtgtcgagc
    11521 acggtgccca gcgcctgcgc aaggcggtag atcggctggc tgcccggccg gagcgaggcg
    11581 gctcgccacc gggtgccacc gccgcgaaga aagccggtct ccagcgcagg cagcaggccg
    11641 gcgcgcacga gcgatgactt gccgcatccc gactcgccgg cgatgacgac cagccgcgcg
    11701 gttcgcaggc gctcgagcag accatcgatc tgctcgtcgc ggccgaagaa gatcgccgtt
    11761 ctctcgacga cgatcagccg ctcgggccgc tcgcggctcg cggtctggat cgccgcgaac
    11821 aggtctggat gagcatgcca gtcggatgcg aagaacgccg gcgacaggac gaggaccacc
    11881 atcgccgagc gctgcaccgc gatcgcccgt tcggcaggcg ggagatggcc gggcgtgcgc
    11941 gcgtggatgc gcggctgcgc gaccccccgc atctgcaggt ccttcgatac gcagtcgacc
    12001 agctcgtcga tccatccgct gccgccgccg cccggcagca acgcgttgtc cgcttctgca
    12061 aagcagaaca tcacgtcgtg gtcatgggcg ccgttcacga gggtcgcagc ttcttgagat
    12121 gggatcgtgg cagcttgctc atcttggcac cgcgcccttg gccgcccgcg ccgcgcgcag
    12181 ccacgcgagg agatcgtccg ggttgccgac ctggccatgg gtctcgcagt acctgaggag
    12241 cgcaccgagg aagcccttcg tggaggcctc cttgatacgc cccacggccg cgcggctgcc
    12301 gttgtagctc caggtctgcc tggtgacctg cggggccagc gtcacgagcg cctcgagcgc
    12361 cggctcggcg gcggcgaggt cctggctctg gtacagcgcg gcgacgagca cgaaccgcat
    12421 ggcgagctcg acgtcgggcg tcgaccgctg gatggcgcgc gcttccagga cccggctggc
    12481 gagcagccgg gcctggatga agcggttgtt tgcccacgcc gtctgggcga ggttggccca
    12541 gccgaccgga tctgtcggat cgacggcgag gctcatccag cggtaccctt ccttcaagag
    12601 ctcgagctgc tcattggcga tctcgccggc gacgcgcgcc gtcttgcgat agtcgggcgc
    12661 catcggcgag atcgcgcgca gcgcgttgag cgccctggca ggctggccaa aatctcgttg
    12721 gatctcaccg aaatactgca acgccagatc gttcgtcggt tcgagatcga gggcgtggtc
    12781 gagcacgagc ctcgccttgt cgcgtttctg ggtatggcga agaaagtccg caaggacggc
    12841 cagtgcctcg acgtcgtcga tctgtcgata cacgcgctcg atccacggcg tgacgacgtc
    12901 gttcatgtcg gtcgcctcgg atcgcgaggc gatgaagcgc agcagcacct gctggtgcag
    12961 gcggtccact cgctgcagct ccccgacggg ctggtgcatg cggtccgctc gctgcagctc
    13021 ctcaacgatg cctttcagcc cctcgatcgc cgcgtgccgt tcgccgctcg ccatgtggtc
    13081 ttgcacgcgc ttgacggccg cgaggttggc cgtgatggcc gcggcgtggg tctcccagtg
    13141 cgcttcgtcg gaggccgggg gggtgagccg gaacgcgtgc tccgccgagg gctgcgcgcc
    13201 tgccaccgcg aactggcgcg cctgctcgga cagctgaagc gcggcgatcc gggatgccag
    13261 cgccgcgggg tcgagcacga tgttggcatc gctgcgccgc gcgtgctgca ggcatggctc
    13321 gccgagctgc accgtccgga gccgcgccgc ggcgagctcc ttgccgaagc gctcgctgcc
    13381 atcctccagc aggctcgtcc ggctcgcgac gttcttcgcc accctgaaga gccgtccggt
    13441 gatgccgtcg atcgcatggt ggatgatctg cgctgatcgg aagcgagcga actcttgctc
    13501 gaacgcggtc ggatcctgat acgccgcgat ggccaggctg tagtcgccgt acaggctgaa
    13561 cccatgctgg agcatgtgcc ggccctcgtt ggcgcgctgc agcggggtgc gcggttcgcg
    13621 gcagccctgg ttggcgtagc gcacgcactc gctctcggtc aggtagatcg gcgtgcgatc
    13681 gcgggcaagc agccgcaggg cgtcgctgtc gacggcatag aaccggacga ccccatcatg
    13741 ccctccggcc acgaacgact tgccgtcggg cgagaacgcc acgctgtcgg agccgaactc
    13801 cgcgtccggg aatccctcct gcgggagatg ggagctggtg ttccacagcc ggaccagatc
    13861 gttgccgctc gatgcgagca gcttgccgtc cggggtgagc gcgaggccgg tgatgctctc
    13921 gtggtgtccg cgcagggttg ccaggtggct cctggcatcg atcttccaga tcttgatcgt
    13981 cttgtcctcg ctcgacgacg ccagccactg tccatccgag ctgaagcgca gcgccaggat
    14041 cggtttgcga tgggcctcgc cgtcctccga cggtcgatcc tccccgggtg cgggtgcgcc
    14101 gaggatcgta ctcagcttgc cactgctggc cttccacagg cagatcggcc gcgcctcggg
    14161 ttcggcgtcg atggcgatgc cacattcgct ggcgatgacg tcgtccctcg gactgatcgc
    14221 caccgcatgc acctgcgtgc ccgtgtcgat ggtgaaatcg ggtccattgc ggctggtgtg
    14281 ccacacggcg agcttgtgac cggccgccac gaccaggcgg tccgcggtgt gaaacatctg
    14341 gagatcctgc acgtcgtccg gtagctcgag cgcgggctcg ctcggggcgg gcgatgcgag
    14401 gtcccagaac cccacgtgat gtccgcgggc gaacgccagc ctggtgcccc gagcatcgag
    14461 gtccatcgcg cgtaccacat cgtcgccccg cagctgcgcc accaggcggc cctcgcggct
    14521 ccacagcgac accgcgccgt cgtcgccggc ggtgacgatg cgatctccgg ccgggtggta
    14581 cgcgatgcgg cggatcgcgc cctcggctgc gcggaacgtg aggagctcgc cgccggcctc
    14641 gacgtcccag atcttggcgc tgccgtcccg tccggtcgtc accagcgcgc ggccgtccgc
    14701 gcggaacgcc acggtctgca gccacgatgc ctcgtcgtcg cgggacgcgg cgagctcggt
    14761 ggccggcctg ccgtcgctgt gcagcggcac gtccgcgggg gtcgagaacc gctgccggcg
    14821 atcgcgggca tccaggacgc ggagcgtgcc gtcgagcccg gtggtggcga tccgcgggct
    14881 atccgggctg aacgcgacgg cggtgatctc gcggctctgc agcagcgagc cctcctcgtg
    14941 atgcgtgatc gcgtcccaca gcgtcacgcc gtagctgccg gccgcagcca cgtagcgccc
    15001 gctggcgtcg aacgtgatcc gcgacgtgcc cggcgagcgc gcgaacagct ccagcttctt
    15061 gcctcgctcg gtgtcccaca ggacgatgcg cccgtcgctg cccaccgtgg cgagccgctg
    15121 gctgtcatgg ctgaacgcga gcgcgatgac ggcgccgagg tgatcgatca gcggagggcg
    15181 cggcttgccg tgttcgacgt cccacagcac ggcgctgccg tcgtcgctcg cggtggcgag
    15241 ccaccggccg tcggggctga acgcgatgtc gttgaccgcc atcacctgtc tgcgtccggt
    15301 ccacagctgc gtccggccgg acaggtccca caggtccacg gtgccgtcat cgctcgcggt
    15361 ggccgccagc gcagtgcgct gagcggtata gatcgcgagt cgatgcggaa tcgcgccgta
    15421 cagcgcgtac aggcgccgcc cggtcgcgat gtcccacacc gcgagaccgt cgcccacgcc
    15481 gacggtgacg atctgctggt cgtccaggaa cctcgcatcg tcgaagctgc cgagcgaagg
    15541 aaacaccatg ctgcgaaagt gcgaggcgcg cagcgcgccg tgcagcgccg tccctgcctg
    15601 cggatcgggg tcgtcgccct gggcctccag cgcgagctgg agtgcgcgca ccggctccag
    15661 gcgcgcgccg aaggcggcag cgctgtagat ctgttgccgc tgcagctcgg cgtgccggcg
    15721 ctcctgctcg cgttgctcgc tgttgcgcgc gagaacgtac acgccggcaa aaaacagtcc
    15781 ggccgccagc acgatcaccg agcggttccg ccactggcgg atccgctggc tgcgcaggct
    15841 gtcgcgcagg aatcgctgcg cgttcgcgaa gtcctcgggg tgctcggagt aacgccgggc
    15901 ccatgcttcg gtcggctgga gcgctgcttg ccaggtccgc gcgtggtcca tcgccttgtc
    15961 gctcagcaga tcgccgttgc cgtggtcgcg cgcgctggcc tcgtggagcc agcgccggta
    16021 ctgcttggcg tcgtcggcct ccttggcgat ccactcgtcg agtcgcttcc agtgccggaa
    16081 caggctctcg tgaccgacgt cgaggaccgt gtcctcggcg agcgcgatct ccggcggcgg
    16141 catcagcagc gagtgctcgg accggcgaaa cgcatcggcg acggcgatca tggcctcgac
    16201 gctcacgccg gcgatctcgg cggcttcgcg gaccgtgcac ggcctccggg tatcgctgcc
    16261 gctggcgttg ccgtcccaca gcgcgcagaa caaccggctc gcgatccgct gctgctcggc
    16321 gctgccgagc gcgcggtaga cctcgtcggc gtgccgcgac agcgcctcgc cgatcccgcc
    16381 cagcgcggcg taatcgtcga tcgtgagctt gatcgggccg gcggcttctg gcgcgcgctg
    16441 cgcggcgcgg tcgcgggcct gccgccacat cgcgagcagc gcgtgctcga gcacgggcag
    16501 ctgatcctgg cgcaggccga tctcgttgat cagctggttg gtcagcgtcg agtcgacatc
    16561 gccctcgaac acgcgcgccg gattgacgat ggcctcgcgc agctgctcgc gggtgagccg
    16621 ggggaccagg tactggctgc cgttcagtgc ttcgggcaag ccggcgaagc tcgcgcaccc
    16681 tccgaggtag tcggtccgca gggtcaggac gacgcggacc gggatcgtct cgtcggccac
    16741 cgtctccagc agcagcgcca cgaacgcgtc ggcctcgtcc ggatcgatgc gcgcgcggaa
    16801 ccggatcagc tcctcgaact gatcgaccag gatcaggagc tcggtgccat cggggagctg
    16861 gaagtcgcgc aggatatcgg ccaggctgcg cggaccgtag tgcagccggg ccgccagcaa
    16921 cggcgcgtcc ttcgccggat cggacgacag cacgtgggcc agtttctgcg cgaggtgatg
    16981 gatcggccgg ttccccggtc gaagatcgac gatccgccag cgcgggccgc cgccgtgcag
    17041 cagaccggcc tccaccgcgg gcagcaggcc ggcgcgcacc agcgatgact tgccgcaccc
    17101 cgattccccg gcgaccgcga ccagccgctc gcgctccagc cggtcgagca ggcggtcgat
    17161 ctgctcgtcg cggccgaaga agatggcggc ctcgtcctgg cgaaacgccc ggagcccggg
    17221 gtaggggctc agcgccaccc ggctggccga agccaggcgt gcgccgtcag ccattggcga
    17281 tctcctgcag gaactcgcgg aactcctggg gctcgccacc gtcgcggccc cggatcatgg
    17341 gccagtgggc gccgagcagc tccggcttgt tctcgggcgg accgtcgtag atccgcagcc
    17401 gcttgaggtt cttcttcagc gccttgaaca gcccctcgaa cttgtagtag cgcgacagcg
    17461 gccgctcgct gctctcggcg ccccagtaca gcacgacgcc gttcaccgcg cggagcacgg
    17521 tccgtagctc ggagtccttc ggctggtgcc aggcccaccg gatgttatcg tatcgaccga
    17581 agatctcctc gagctgggcc acctggtcgc ggctggcgtc gacgaacacg ctcggcagcg
    17641 tggtctgcgc gcgcagcgca gcgcacaccg cctggcagaa cgcggggatg ctgctcgcct
    17701 gcaccgtgtc gcgcagcagg agctcccgga gcctgggatc ctgcaggcgc atgacgtcgg
    17761 tcgaggggtc gcgccactgc tggatctcgc ggcccttgcg caccgcgatc tcgagctgcc
    17821 gcgccaccgc gccgggcacc tgatccgggg cctcgggcgc cgttcccagc agctgcacga
    17881 acagtcgcac gctgtcgatc agccgggcgg tctcggcctc gaactgcgcc ggatccgagg
    17941 gcagccggcc gtggggcacg acctcgaagc cagcctgctc gagatagcgc agcacttcga
    18001 ggcggcgagc ctcgacgtcc cgggtggcct cggcgaggaa cacccgctgc cgcgcgccgg
    18061 tgtccggtcc agccgccgca gaggccgcgg caccggccgg gctcagctgg gatgcgaggg
    18121 ccaggatcag gtcgaacacg accgcgaagt aggcctgctg atcgggctcg gcggcgaggc
    18181 tggggaaccc gagggtgcgc ggcgtaccgt gcacggagcg ccagaacacc gcccggcggt
    18241 gggcatcgaa ctccgccgcg atctggcgcg ggacctcgga cttctcgacg atgaccaccc
    18301 gctggggcgc ggccgtggcc acgctgcgga tcgcctcggc gagcgcctcg ctgcgcaccc
    18361 agtcggagtt gaagaacgcg ggcgacagca cgaccacgat ggtcgcggat cgccgcacca
    18421 gctcggcgcg ttccgccgcc gggaaatggc ccggtgactt ggacagcacg cgcggctcgg
    18481 cgatgccgcg cccgccgagg agcttcgtga cgcagtccac cagatcgtcg acccagccat
    18541 tgggctggtt cccgaacgtg gcgttgtcct cgtctgcaaa gcagaagaac acatcggttg
    18601 gcatggcggt cggcgttctc cgtgtcgcgt cagggtcgat gggtgatggg gtcgctggct
    18661 tgcggtggtg ccgccgcggc catgcgcttg agctcgctgg cgagctcgag ggcgaggccg
    18721 tggaggcggt ggtagtactc cggctcgtcc ggtctcggga ccgggacgcc gagcacacgc
    18781 ggtgcgcgat cgcggcggtc tcggacccag aacggatagc cgagcaggtc gcgaagctgt
    18841 tccggcttcc ggtcgcgctc gatcggcatg cgctcgacca cgaacaggcg ccgcgtgccg
    18901 gggccggcca gctggacgaa ctcgcgatac tcgctccagc accagtccga ggccagataa
    18961 ccctcggaca gcacgatgag cagcgtcgcg gagctgcgca gggcaccgaa gatctccggc
    19021 gtcagctccg catgaccggt gagctggaag tcgcgccaca ccgcaagcac ctcggcgcgg
    19081 ccgagctgct ccgcgagcaa gatcttcagg gcatcgccca gcaggctgac ccagccatcc
    19141 cgcgcccctg gaagcggctg gtcatcgacg tgggcatagc tgacgaagac gtcgttctga
    19201 aaaccgggta cgtacgtcat cgcgactccg ccacgaccgg gatggccggt ctgccgtgcg
    19261 cggtcgcagc gcggcagcga gcggacgcga tgtagccagt gcggtgcccc cgtgacatcg
    19321 gcagatgagg cgatccgtcc tggcaaacca cgacagcctc atcgtgagaa gcatcgatcc
    19381 cagcgctggc ccggcgctgg ctggggcgtt ttgcctcgga tcagggcttc agcgcgccgt
    19441 cgagcagcgc gaacagcgtc tgccagtggc gctcggcggc cgccgggtcg tggaccggag
    19501 tgtcgctcgg cacccagccg tggcgagccg ggtaggtctc gacggtgtgg cggacgccgg
    19561 cgtcgcgcag cgcggtctcc aggcgggcct tcatcgcgtc gggaaagctc gggtcgtcgc
    19621 tggcaccgcc gacgtagacc tgggcctgga tcctgggcgc ctggaggtgc gggctgtcgg
    19681 gggcgtcggt cgcgaggtgg cccgggtggt acgcggcgac cgcggcgaac cgatcgggga
    19741 agaagccggc cgccgagatg gcgaggccgc cgcccatgca gtagccggtc gcgccgatct
    19801 ggccgggggc gacggcgggc tggccggcga ggaacgcgag gacggcgcgg gtgtcgcgca
    19861 tggcgttggc ctggccgagc ttgcccatga acttctggaa cagcgtctga cgcacctcgg
    19921 gatcgccgaa cacgcgcctg gggtccatcg gctcgtaggg gccgctgcga taatagaggt
    19981 cgggcaggat cacgtagtag ccgtgcgcgg cgagccgcgc gcccatctcg aacagggccg
    20041 ggcggatgcc gaggccgtcc atgaagaaca ggacggccgg ccacggcccg gcgccggatg
    20101 gctgaaacac gtgcgcgggg caggtgccgt cctcggtcgt gatgtcgatg gttgctggca
    20161 tgcgcgtgag tgtggcaccg ggttgcgcgc tccggacctc cggacacacg tcacaacgac
    20221 gtggaacgcc atgtcgccgg aggccgtcag gcggtcttgt cgagcgcgtc gcccagcacg
    20281 ctgaccaggc ggtcgatctc ggacttctcg atgacgaacg gcggggcgag ctggatcgtg
    20341 tcgccgccgt agcggacgta gacgccggcg gcgaagcact tcatcgcgac ctcgaacggc
    20401 cgcctggcgg gctcgccggg caccggggcg agggtgaggc cggcggccag tccgaagttg
    20461 cggatgtcgg cgacgtgctt ggcgcccttg aggctgtgga cggcgttctc gaagtgcggg
    20521 gccagcgcgg cgacccgctc gatcgccttc tcctggacca gcagatcgag cgcggcgacg
    20581 ccggccgcgc acgcgatcgg gtgggcggag taggtgtagc cgtgggcgag ctcgaccatg
    20641 tactcggggc cgccggtggc catgaacgcg tcgtagatct ccttcttgac gaccaccgcg
    20701 ccgagcggct gggtgccgtt ggtgatctgc ttggcgcagg tgaggaggtc gggggtgacg
    20761 ccgaacgcgg ccgcgccggt gtaggcgccg caccggccga agccggtgat cacctcgtcg
    20821 aagatcagca ggatgttgtt tgcggtgcag atctcgcgca ggcgctggag gtagccggcg
    20881 ggcgggatga tgacgccggc cgagcccgcg aacggctcga cgatcacggc ggcgatgttg
    20941 gaggcgtcgt gcagcgcgat cagccggagc agatcgtcgg cgagctcggc gccgcgcctg
    21001 ggcatgccgc gggtgaacgc gtcggccttg tcgtagaccg cgacctgggt gtgcggcagg
    21061 tgatcggcct cgatgccctg gccgaagatc ttgcggttgg cgccgatgcc gccgaccgag
    21121 atgccgccga agttgacgcc gtggtagccg cgctcgcggc cgatcaggcg ggtcttggtg
    21181 ccctggcccc tggcgcgcca gtacgcccgc gcgatcttga gcgaggtgtc caccgtctcg
    21241 gatccggaac aggtgaagaa gacatggtcg aggccgtccg gggtgagctc cttgatcttg
    21301 ttggcgagcg cgaacgactg ggggtggccg tactggaacg ccggcgcgta gtcgagcgtg
    21361 gcggcctggc ggctgatcgc ctcggtgatc tcgcggcggc cgtggcccag gccggtgcac
    21421 cacagaccgg acaggccgtc gaggatcttg cggccgtcgg cgtcggtgta gtacgcgccc
    21481 tcggcggcga cgagcatccg ggggtgggcc ttgaactcgc ggttcccggt aaacggcatc
    21541 cagtgcgcgt cgagccaggc ggcgtcggta cgagtcagct tctggttggg ctggttcatg
    21601 gggtccgtcg attccgagga aaacctagcc ccatgccgcc cgggtccgca accgccgaag
    21661 tcgccctcgc cgggagtcgt cgtcggacag tggttacggc tcgctgcgtc gtcgatcggg
    21721 cgatggcgac ctgcggcacc cgcggagcgg gcgatcgcgc cgtcgcgccg gttgtcggac
    21781 cgcaggcaac gctgtcggaa acccacacag tgccggggca taacgccccg caaggacccg
    21841 gccgatctgg ctgcgcccgg tggcatcgcg catgcaaact cgcgcgtaca atggcgaata
    21901 ggtatcgatc gagcgccctc gtcggcgcgc tgatcctctc gaccgccggt cgcacctggg
    21961 ccgaccgcag catcaccctg gaggacgcac tcgcgctggc gcgcacccac aaccgcgatc
    22021 tccggtccgc gcgggagcgg gtcgtcgagg cggaggcggg cgtggcgcag gcgcgagcgg
    22081 ccctgctgcc gaccgtgtcg gcccagggca agtacacccg caactacaag gaggtcgact
    22141 tcgacgccat cgcgttctcg cagccgacgt tcgacgtcgc caacgcgatc cggacctcga
    22201 ccaccaaccc cgccgaggcc gcggcgatcc agggcgtcgt cgaccgcgac cgggcgctgc
    22261 tggccggcca gccgacggtg gtgatccaga agctgaacca gctcgacggc gtggtcagcg
    22321 cggcggtgcc gctggtgtcg ccgcccgcgt actacgggct gtcggcggcg cagtccagcc
    22381 gcgacgcgag cgccgcgggc taccacgtca ccgaggccgg cgtgctgctg tcggtggcgc
    22441 aggcctactt cgcggcggcc ggcaccgacg agctggtgct ggcgcgccag gacgcggtca
    22501 aggtcgcgac cgagacgttc gacgtcgccc gggcgcgggt cgccgccgag ctcgccaacc
    22561 aggtcgagag cacgcgggcc gagaccgcgc tggtccgcgc ccagcaggac ctcgccgagg
    22621 ccgagaacac gcgcggcgcg gcgtaccgcg cgctcgccac gctgatcggc acgcacgagc
    22681 cgatcgcggt gcaggcgacg aggacgccgc ccgaggagcc cggtccgttc gaggcgctgg
//
LOCUS       DASVOR010000026         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_350996,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000026 DASVOR010000000
VERSION     DASVOR010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350996"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1160
                     /locus_tag="VF516_00600"
     CDS             393..1160
                     /locus_tag="VF516_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEX8106186.1"
                     /translation="MKAVILAAGCATRLRPYSDDTPKTLLPVGGVPILRRTITSLLRV
                     GFDQFVIGTGYLEHMVREAVGGWFPGLDVTYVINPEYQTTNNAYSLLLMRDHVEHDGF
                     ILLDGDVVFDLGVVEELIERGPDCLAVRSVGEIGLEEVKVTADNEDRVLAIGKHVPVR
                     SAMGESVGIELFSAASSKKLFAALHQRVREHGLVNEYYEAAFQQIIDSGVTLYGVDIG
                     SMYATEIDTVEDLTAANERLAQRPAFDVRVDGLRLAV"
     gene            1224..1955
                     /locus_tag="VF516_00605"
     CDS             1224..1955
                     /locus_tag="VF516_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106187.1"
                     /translation="MRWLLICLAIAGCARAGKENSIIGGLTDARPRGDAETVPLPDAS
                     PFDAPPQQVTLTQTANNAITKDNSFACVDAGSTLQNSYYRVFKLADYEITTTLHVIQV
                     DFGIQTAAAGGGAGSQPATVHIGSYAGTPGGTTLDLSQVRMINSANIKIPDGSATGMT
                     VPITADIAPGTSVIVELAIPDGTAAGNKFFVGTNTDTERAPGYTSEPDCGVTQPTSMQ
                     SIADDNMFGNVHLVMTLTGMTDTPN"
BASE COUNT          343 a          722 c          675 g          285 t
ORIGIN      
        1 cgggcgccgc gccaggccgc cagctgatcg cggacgcggt cgaccaccag cacgcgcgtc
       61 gcaccggccc gccggacgac ccggacggcg cgctccccgg cggtcagtcc ggcgaccacc
      121 tgctgcgaca gcgcatcgaa caggacgacc gcgtcgagct ccatcgcagc cgatacctgt
      181 acaccattcc cccgccggaa cgccccccga tccggacgat gaaccggagc cggctccgga
      241 ggatgaccgt cggacgatgg caggacagtg acaggaattg gtcgcctcga aggcgctccc
      301 ggggcggatc tggtgaaagt tggacagccg gtgggctcaa gtagtggaaa cccatacgcg
      361 catgccgatt gcacggagcc ggtcgtccat ccatgaaggc tgtcatcctt gctgcgggct
      421 gtgcgacgcg gcttcgtccg tactccgacg atacgcccaa gacgctgttg cccgtcggtg
      481 gcgtcccgat cctgcggcgg acgatcacca gcctgctccg ggtcggcttc gatcagttcg
      541 tgatcggcac cggctacctg gagcatatgg tgcgcgaggc ggtcggaggc tggttcccgg
      601 ggctcgacgt cacctacgtg atcaacccgg agtaccagac cacgaacaac gcgtactcgc
      661 tgctgctgat gcgcgaccac gtcgagcacg acggcttcat cctgctcgac ggcgacgtcg
      721 tgttcgacct cggcgtggtc gaggagctga tcgagcgcgg cccggattgc ctcgccgtgc
      781 gctcggtcgg cgagatcggc ctcgaggagg tcaaggtcac cgccgacaac gaggaccgcg
      841 tgctcgcgat cggcaagcac gtcccggtgc gctcggcgat gggcgagtcg gtcggcatcg
      901 agctgttctc ggccgcgtcg tcgaagaagc tgttcgccgc gctccaccag cgggtccgcg
      961 agcacggcct ggtcaacgag tactacgagg cggcgttcca gcagatcatc gacagcggcg
     1021 tcacgctgta cggcgtcgac atcggctcga tgtacgccac cgagatcgac acggtcgagg
     1081 atctcaccgc ggccaacgag cggctcgccc agcgcccggc gttcgacgtc cgcgtcgacg
     1141 gcctgcgcct cgcggtgtga cgtaggtcgt cccggccgcc gttcgcgttg tgcgaggcgc
     1201 accgacctcg ctaccatgcc gccatgcgtt ggctgctgat ctgcctggcg atcgcggggt
     1261 gcgcccgcgc cggcaaggag aacagcatca tcggcgggct gaccgacgcc cggccgcgcg
     1321 gcgacgcgga gaccgtgccc ctgcccgacg cctcgccgtt cgacgcgccc ccgcagcagg
     1381 tgacgctgac ccagacggcg aacaacgcga tcacgaagga caactcgttc gcctgcgtgg
     1441 acgccggctc cacgctccag aacagctact accgggtgtt caagctcgcc gactacgaga
     1501 tcaccacgac gctgcacgtg atccaggtcg acttcgggat ccagaccgcc gccgccggcg
     1561 gaggcgcggg gtcccagccc gccacggtcc acatcggcag ctacgccggg acgcccggag
     1621 gcacgacgct cgacctctct caggtccgca tgatcaactc ggcgaacatc aagatccccg
     1681 acggcagcgc gaccgggatg acggtgccga tcacggcgga catcgcgccg ggcaccagcg
     1741 tgatcgtcga gctcgcgatc ccggatggta cggcggcggg gaacaagttc ttcgtcggca
     1801 ccaacaccga caccgagcgc gcgcccggct acacgtccga gccggactgc ggggtcacgc
     1861 aaccgacctc gatgcagagc atcgccgacg acaacatgtt cgggaacgtc cacctggtca
     1921 tgaccctcac cgggatgacc gacacgccga actgatcccg gcacgggcat acgcggcgcg
     1981 ccggtcggcg gcgatgtcgt cgagcaccgc gcggatcgcc gcgtg
//
LOCUS       DASVOR010000027         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_146401,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000027 DASVOR010000000
VERSION     DASVOR010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146401"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..479)
                     /locus_tag="VF516_00610"
     CDS             complement(213..479)
                     /locus_tag="VF516_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106188.1"
                     /translation="MATLIDNPKRARQLARAIASDLTLYHEPKILEGIANDSLFEAMA
                     DEINEGRELFKSRVTEEIFGQHIYERAIVDVLVKSKGHVKSKIW"
     gene            complement(513..2054)
                     /locus_tag="VF516_00615"
     CDS             complement(513..2054)
                     /locus_tag="VF516_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8106189.1"
                     /translation="MVAIGGGKGGVGKSLVAANVGIFLATLGKRIVLVDAAFGAANLH
                     IFAGVPRPSRSLSEALAPGGPRLGELAVPTHVPGVRLIGGVYDPPWVAEPAPELVQLL
                     ADQLRQLAADWVVVDLGPGIASPTLELFLAADVQVIVAVPDPTSIELMHRFAKAVFLR
                     SLERRGLGHLAQAVGRDPRHLEGGAPSALEVYLGAVERRADEVDALRDAILAFSPHLV
                     INSARSKSDMELGRAVTSAARRRLGMPFRYLGHLEYDEAVWASTRRRRPLLIEHPETR
                     IAKCFERVARGVLAVRPPASDGEVLASDSHYELLEVPPTASFEDIRRANRRIRDIYGA
                     ESVAISGLYDPASLEAVHRRLDLAYTTLMDAAKRKEYDVELFPDGVPMPVTPSVLADL
                     PASRPVVKVDDPVTVAVRPPMPEISAGTEFSGPLLRQIREAIGVELREIAERSKIGMA
                     YLSALEGESFHKLPAAVYVRGFLAEYARALGLDGERVKQTYLARFRAARGPSADDEDE
                     PRPKS"
BASE COUNT          352 a          894 c          804 g          310 t
ORIGIN      
        1 cgctgctcga gctcgatcac ccggtcaccg gcgcgcggct gcgcttcgag tcgccgctgc
       61 ccgccgacct cgccgcggcg ctggctcggc tgcgcgcgcc gcctacttga gctcgaccga
      121 gctccagaac gccaggcgcg cccgcgcacc gggcgcggcg ggacggagcg ggacggggcg
      181 gggaagacgc ggcgccgcga ctctgtcgcc ggctaccaga tcttcgactt gacgtgcccc
      241 ttggacttga cgagcacgtc gacgatcgcg cgctcgtaga tgtgctggcc gaagatctcc
      301 tcggtcacgc ggctcttgaa cagctcccgg ccctcgttga tctcgtcggc catcgcctcg
      361 aacaggctgt cgttggcgat gccctccagg atctttggct cgtggtacag cgtgaggtcc
      421 gaggcgatcg cccgggcgag ctgccgtgcg cgcttggggt tgtcgatcag agtggccatg
      481 accgcggatc gtgagagaac cccgagctgc ggtcaagact tcggacgcgg ttcatcttca
      541 tcgtcggcgc tcggcccgcg cgccgcccgg aaccgcgcca ggtaggtctg cttgacccgc
      601 tcgccatcga gcccgagcgc gcgggcatac tcggcgagga agccgcggac gtagaccgcc
      661 gccggcagct tgtggaacga ctcgccctcg agcgccgaca ggtacgccat gccgatcttc
      721 gagcgctccg cgatctcgcg cagctcgacg ccgatcgcct cgcggatctg gcgcagcagc
      781 ggcccggaga actcggtccc cgccgagatc tcgggcatcg gcggccgcac cgcgaccgtc
      841 accggatcgt cgaccttgac gaccggacgc gacgccggca ggtcggcgag caccgacggc
      901 gtcaccggca tcggcacgcc atcggggaac agctccacgt cgtactcctt gcgcttggcg
      961 gcgtccatca gcgtcgtgta cgcgaggtcg agccggcggt gcaccgcctc caggctcgcc
     1021 gggtcgtaca gcccgctgat cgcgaccgac tccgcgccgt agatgtcgcg gatccgccgg
     1081 ttggcgcgcc ggatgtcctc gaagctcgcg gtcggcggca cctcgaggag ctcgtagtgc
     1141 gagtccgacg ccagcacctc gccgtcgctg gccggcggcc gcaccgccag gacgccgcgc
     1201 gcgacccgct cgaagcactt ggcgatccgg gtctcggggt gctcgatcag cagcggccgg
     1261 cgccgccggg tcgacgccca caccgcctcg tcgtactcca ggtggccgag gtagcgaaac
     1321 ggcatgccga gccgccggcg cgccgccgag gtcaccgcgc ggccgagctc catgtccgac
     1381 ttcgagcgcg ccgagttgat caccaggtgc ggcgagaacg ccaggatcgc atcgcgcagc
     1441 gcgtcgacct cgtcggcccg ccgctcgacc gccccgaggt agacctccag cgcgctcggc
     1501 gcaccgccct cgaggtgccg cggatcgcgc ccgaccgcct gggcgaggtg gcccaggccg
     1561 cggcgctcga ggctgcgcag gaacaccgcc ttggcgaacc ggtgcatcag ctcgatcgac
     1621 gtcggatcgg gcaccgcgac gatgacctgg acgtcagcgg ccaggaacag ctccagcgtc
     1681 ggcgacgcga tccccgggcc gagatcgacc acgacccagt ccgccgccag ctggcgcagc
     1741 tggtcggcga gcagctggac cagctccggc gccggctcgg ccacccacgg cggatcgtag
     1801 accccgccga tcagccggac ccccgggacg tgggtcggca ccgcgagctc gcccagccgc
     1861 ggcccgccgg gcgcgagcgc ctccgacagc gagcgcgacg gccggggcac cccggcgaag
     1921 atgtgcaggt tcgccgcgcc gaacgccgca tcgaccagca cgatgcgctt gcccagcgtc
     1981 gccaggaaga tcccgacgtt cgcagcaacc aggctcttgc ccacaccccc cttgccgccc
     2041 ccgatcgcga ccacacgcgg gatcatggag ggggcacata accgcgacgg cgcgatccgg
     2101 tcaagcaccc ggtgacagcc cgttcagtgc gcacggcgat ttaccgccga agcggccggg
     2161 cgggggaccc gttcgaccgc ggatctttcg cagacgacac acgatcggcg ggccgccgcc
     2221 cgcctgcaca gcgctcgctg cacaaaaaac ctcctcacat ccggtggcag ttctccgtcg
     2281 agaaactcga tgcaacccgg gggcgtacgc gacgtccagc ctcgcgatga tccgccgatg
     2341 gaccgccctg gcagtcatcc
//
LOCUS       DASVOR010000028         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_204912,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000028 DASVOR010000000
VERSION     DASVOR010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204912"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VF516_00620"
     CDS             complement(<1..592)
                     /locus_tag="VF516_00620"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8106190.1"
                     /translation="MVDGSHLETPTWVTSFAGLPRTVNVRRCRLEVVAGPDAGKRIEL
                     AQPSIMIGRTGADLTVNDPKVSGLHCELRLQADGYRIRDLGSTNGTHVKGVRVVDGFI
                     PPGATIQIGKSAIVFDPLDDSVQVPLWHDSRLHGLIGGSAAMRHLFDMINRFAQSDAT
                     VLIQGETGAGKEGVADAIHQCSPRRDGPFVVLDCSAI"
     gene            complement(616..1509)
                     /locus_tag="VF516_00625"
     CDS             complement(616..1509)
                     /locus_tag="VF516_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8106191.1"
                     /translation="MAMTRFLTATLLVAACGAPAPLPTELNPTEATSTRVAKEPPPPV
                     EFTGVITTRDSKMISAKVQAPIKAFHIQPGDRVKAGAVIAELETTELQNRLVQAQANQ
                     QAAEMEAGAYGAQIGALRQKLIAESRLQRLGVSSTMALRSAQAELGQVGAQAAAASAK
                     ALSAKAEVEQARKNLESARIVAPIDGTITNIRVHEGQGVQFGTPLARVFDTSDMRVKF
                     AVPRDHVSEVRPGQRVEVTVEGNPRPLWAVIITKAEAQEPPINFTVVEADIDDTRLAP
                     GELRVAAVARVRIADARGAKR"
     gene            complement(1430..>2904)
                     /locus_tag="VF516_00630"
     CDS             complement(1430..>2904)
                     /locus_tag="VF516_00630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEX8106192.1"
                     /translation="GDLMMRVDSNGIVRETVTGKSMSAVLDGVFVLLYAIVIFYVSPL
                     LGAITIAMSAAEALVFLCARPTYQRLQAADLDKQAKTHSYMIQMLGGMETLKCAGAER
                     LGVEKWSNLYTDQLNVTMRRARATAYVDGLRSAVASLGPLLILTVGTVNVMSGKMTLG
                     MMLAMNSLATSLFTPLSALVSSALDLQMLKSHMDRIDDVLQTPIEQDRDAVAAPPRLR
                     GQVSVRNLSFKYGDQAPLVVQNVSLDITPGMSVALVGPSGSGKSTLLNILAGLYKPVT
                     GEVHYDGRPLHDMDVRAVRQQIGIVPQHPFIFGGSLRENVALTAPGAQLDRIIAACKV
                     SCLHDDITEMPMGYDTVISDGGGSLSGGQRQRVAISRAVIRNPSLMLLDEATSALDNA
                     TEKRVIENLERQRCTRITVAHRLSTVRNADLIVVMDKGRIVEQGNHHQLLAAGGLYAR
                     LVAASQNPTTELPPHGHDAISHGNAARGSVRSTRSAAHRA"
BASE COUNT          394 a          940 c         1088 g          482 t
ORIGIN      
        1 ggatcgccga gcagtcgagc acgacgaacg ggccgtcgcg gcgcggcgag cactggtgga
       61 tcgcgtcggc gacgccctcc ttgccggcgc cggtctcgcc ctggatcagg acggtggcgt
      121 cggactgggc gaaccggttg atcatgtcga acaggtggcg catcgcggcg ctgccgccga
      181 tcaggccgtg caggcgcgag tcgtgccaca gggggacctg gaccgagtcg tcgagcggat
      241 cgaacacgat cgcgctcttg ccgatctgga tcgtggcgcc cggcgggatg aagccgtcga
      301 ccacgcgcac gcccttgacg tgggtgccgt tggtcgagcc gaggtcgcgg atccggtagc
      361 cgtcggcctg caggcggagc tcgcagtgca ggccggagac cttgggatcg ttgaccgtga
      421 gatcggcgcc ggtgcggccg atcatgatgc tgggctgcgc gagctcgatg cgcttgccgg
      481 cgtcggggcc ggcgacgacc tcgaggcggc agcggcgcac gttgaccgtg cgcggcaggc
      541 ccgcgaacga ggtgacccag gtgggggttt cgagatggct cccgtcaacc acgtctactg
      601 ctgtttgtat cacgctcatc gcttggctcc tcgtgcatcg gcgatccgaa cgcgggcgac
      661 cgctgccacc cggagctcgc ccggggcgag cctggtgtca tcgatatccg cttcgaccac
      721 ggtgaaattg atcggcggct cttgcgcttc cgctttggtg atgatcacgg cccagagcgg
      781 ccgtgggttg ccttcgacgg tgacctcgac gcgctggccg ggccggacct cgctcacgtg
      841 atcccggggg accgcgaact tgacgcgcat gtcgctggtg tcgaacaccc gggccagcgg
      901 ggtgccgaac tggaccccct ggccctcgtg gacccggatg ttggtgatgg tgccgtcgat
      961 cggcgcgacg atccgggcgc tctccaggtt cttgcgggcc tgctcgacct cggccttggc
     1021 gctgagcgcc ttggcggagg cggcggcggc ctgggcgccg acctgaccga gctcggcctg
     1081 cgcgctgcgc agcgccatgg tcgacgacac gccgagccgc tgcagccggc tctcggcgat
     1141 cagcttctgg cgcagcgcgc cgatctgggc gccgtacgcg ccggcctcca tctccgccgc
     1201 ctgctggttg gcctgggcct ggacgagccg gttctgcagc tcggtcgtct cgagctcggc
     1261 gatcacggcg ccggccttga cgcgatctcc gggctggatg tggaacgcct tgatgggcgc
     1321 ctggaccttg gcactgatca tcttggaatc acgcgtggtg atgacgccgg tgaactcgac
     1381 ggggggcggc ggttccttgg cgacgcgggt ggaggtcgcc tcggtggggt taagctcggt
     1441 gggcagcgga gcgggtgctc cgcacgctgc cacgagcagc gttgccgtga gaaatcgcgt
     1501 catggccatg ggggggcagc tccgtggtgg ggttctggga agcggcgacc aggcgcgcgt
     1561 acagcccgcc ggccgcgagt agctggtggt ggttgccctg ctcgacgatc cggcccttgt
     1621 ccatcacgac gatcaggtcg gcgttgcgga cggtggacag ccggtgggcg acggtgatcc
     1681 gggtgcagcg ctggcgctcg aggttctcga tcacgcgctt ctcggtcgcg ttgtcgagcg
     1741 cgctggtcgc ctcgtcgagc agcatcaggc tcgggttgcg gatcacggcg cgggagatcg
     1801 cgacgcgctg gcgctggccg ccggacagcg agccgccgcc gtcggagatc acggtgtcgt
     1861 agcccatcgg catctcggtg atgtcgtcgt gcaggcacga caccttgcag gcggcgatga
     1921 tccggtcgag ctgggcgccc ggcgcggtca gcgcgacgtt ctcgcgcagc gagccgccga
     1981 agatgaacgg gtgctggggc acgatgccga tctgctggcg gaccgcgcgg acgtccatgt
     2041 cgtggagcgg gcggccgtcg tagtgcacct cgccggtgac cggcttgtac aggccggcga
     2101 ggatgttgag cagcgtgctc ttgcccgagc cgctcgggcc gaccagggcg acgctcatgc
     2161 cgggcgtgat gtcgagcgac acgttctgga ccacgagcgg cgcctggtcg ccgtacttga
     2221 acgacaggtt gcgcaccgag acctggccgc gcagccgggg cggggcggcg accgcgtcgc
     2281 ggtcctgctc gatcggggtc tgcagcacgt cgtcgatccg gtccatgtgg ctcttgagca
     2341 tctggaggtc gagcgcgctc gacaccagcg ccgacagcgg ggtgaacagg ctggtcgcca
     2401 gcgagttcat cgccagcatc atgccgagcg tcatcttgcc ggacatgacg ttgaccgtac
     2461 cgaccgtcag gatcagcagc gggccgagcg aggcgaccgc gctgcgcagg ccgtcgacgt
     2521 acgcggtcgc gcgcgcccgg cgcatcgtga cgttgagctg gtcggtgtac aggttcgacc
     2581 acttctcgac gccgagccgc tcggcgccgg cgcacttgag ggtctccatg ccgccgagca
     2641 tctggatcat gtagctgtgg gtcttggcct gcttgtcgag gtcggcggcc tgcagccgct
     2701 ggtaggtcgg ccgcgcgcac aggaacacca gcgcctcggc ggccgacatc gcgatcgtga
     2761 tcgcgccgag cagcgggctg acgtagaaga tcacgatcgc gtagagcagc acgaacacgc
     2821 cgtcgagcac cgccgacatc gacttgccgg tcacggtctc gcgcacgatg ccgttggagt
     2881 cgacccgcat catcaggtcg ccgg
//
LOCUS       DASVOR010000029         2981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_263319,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000029 DASVOR010000000
VERSION     DASVOR010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263319"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF516_00635"
     CDS             complement(<1..258)
                     /locus_tag="VF516_00635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106193.1"
                     /translation="MVGELCPQGAAGRPAVAPLLIRTTQWLDAASEVDGAVERGATPR
                     FVAFGVDGKTAGMFDTVGLADIALGQSVASGTYVGGPPCSSD"
     gene            674..2584
                     /locus_tag="VF516_00640"
     CDS             674..2584
                     /locus_tag="VF516_00640"
                     /inference="COORDINATES: protein motif:HMM:TIGR04551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04551 family protein"
                     /protein_id="HEX8106194.1"
                     /translation="MGAPVVRAQIAPGGLPPAGPGPGAGQGSEEKKEGVAVEAPKTPG
                     LLPTTPALPAPKARRKRWKLLELDGYYRMRTDWFKNFNLGFRDDLTQGGAPFPTALGC
                     KAPMGAPCDDALSSANMRLRLEPTINLDEGTSVHIQADALDNVVLGSTPGDPVLGAGT
                     SVPVANTVPPINTRPPTGAFGNGTQTSVLQGVNSDRPSIQVKRAWAEVAVPFGILKFG
                     RMPNQWGMGILYNAGGYDPINGTYNYDADYGDSVDRASFSLLIPGTNLRAMAAVDWPL
                     TRLVSNQVSTQVGAGQTAMPPGLGHEGHPFDLDDSDDTNSWIGVISRMDSPQEFKDIV
                     DRGEVAFNYGVYFEYKTQAWESNLSNFPPQGPLLVGTLPGQIAYEPTNLKTYSPDVWA
                     KLGVGRFLVEGELVAQLGSTDVVTAAPMTAGAAFTPTHYNIRKLGGAGRFTFKGFEGK
                     LRLGVESGFASGDQYFNTPIGATNLAYGSPFGNTILPNGMVQVAPNDSTLKQFAFNRD
                     YQVDLILWRHLVGAVSNAAYFKPFLQYDITRSITFKVANITSIALHKEATPGDGRVYG
                     TEFDGDLGYASNGVFVGISYGVLFPFGAMDHPDLNPSDSSPPYGHSTTSPNNLGTAST
                     AHTIQSRFVLAF"
     gene            complement(2613..2810)
                     /locus_tag="VF516_00645"
     CDS             complement(2613..2810)
                     /locus_tag="VF516_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106195.1"
                     /translation="MVFGEVGLAGEIRAVPLCDQRLAEAARLGFRRAIVPAQNAAHAA
                     QTAGAIELVPVDRLVTALGEL"
BASE COUNT          461 a         1100 c          994 g          426 t
ORIGIN      
        1 gtcggagctg cacggcgggc cgccgacgta ggtccccgac gcgaccgact ggccgagcgc
       61 gatgtcggcc aggccgacgg tgtcgaacat gccggcggtc ttgccgtcga ccccgaacgc
      121 gacgaaccgc ggggtggcgc cgcgctcgac cgcgccgtcg acctcgctgg ccgcgtcgag
      181 ccactgcgtc gtgcggatca ggagcggcgc gaccgcgggc cgcccggcgg cgccctgcgg
      241 gcagagctcg cccaccaaga cctgccggcc cggccgggtc ggagttggag cgcgttcacg
      301 gtgcgtcacg gcctcttcgg ggaacggacg gtccggctga acccgcggcc cgcacgcaag
      361 cagggcgaga acggcgagaa ggcaggcaac gcggggcagc gtgccgcggt cattagccat
      421 cggatgcacc ataacctgcc ggattcagac acggccactc ggtgacttcg ttgcctcgcc
      481 cgtttgaccc cgctctggtg ccatgcctat agtccgcccg gttttcagtt agcccaattc
      541 ctccgtactt tctatgcatt acaccaaagc tcgtcacaac tcttcgagcg ctccggtgcc
      601 gccctccacc ggagcggctc ggccggtccg ggctcgctgg attccctggg cattctgggc
      661 ctgcgtgctc gtcgtggggg cccccgtggt ccgcgcgcag atcgcgcccg gtggcctgcc
      721 ccccgccggc ccgggtcccg gcgccggcca gggctcggag gaaaagaagg agggcgtcgc
      781 cgtggaggcg ccgaagacgc ccgggctgct cccgacgacg ccggcgctgc cggcgccgaa
      841 ggcccgccgc aagcgctgga agctgctcga gctcgacggc tactaccgga tgcgcaccga
      901 ctggttcaag aacttcaacc tcgggttccg ggacgatctg acgcaaggcg gcgcgccgtt
      961 cccgaccgcg ctgggctgca aggcgccgat gggcgccccg tgcgacgacg cgctgtcgag
     1021 cgccaacatg cggctgcggc tcgagccgac gatcaacctc gacgagggca cctcggtcca
     1081 catccaggcc gacgcgctcg acaacgtcgt gctgggctcg acgccgggcg atccggtgct
     1141 cggcgcgggg acctcggtcc cggtcgcgaa caccgtcccg ccgatcaaca cccggccgcc
     1201 gaccggcgcg ttcggcaacg ggacccagac cagcgtgctg cagggggtca acagcgaccg
     1261 gccctcgatc caggtcaagc gcgcgtgggc cgaggtcgcc gtcccgttcg gcatcctgaa
     1321 gttcggccgg atgccgaacc agtggggcat gggcatcctg tacaacgcgg gcggctacga
     1381 tccgatcaac ggcacctaca actacgacgc cgactatggc gactcggtgg atcgcgccag
     1441 cttctcgctg ctgatccccg gcaccaacct gcgcgcgatg gccgcggtgg actggccgct
     1501 gacccggctg gtatcgaacc aggtctcgac ccaggtcggc gcgggccaga ccgcgatgcc
     1561 gcccggcctg ggccacgagg gccacccgtt cgacctcgac gacagcgatg acaccaacag
     1621 ctggatcggc gtgatctcgc gaatggactc gccgcaggag ttcaaggaca tcgtcgaccg
     1681 cggcgaggtc gcgttcaact acggtgtgta cttcgagtac aagacccagg cgtgggagtc
     1741 caacctcagc aacttcccgc cccaggggcc gctgctggtt ggcaccctcc ccggccagat
     1801 cgcgtacgag ccgaccaacc tgaagacgta ctcgccggac gtgtgggcca agctcggcgt
     1861 cggccgcttc ctggtcgagg gcgagctcgt cgcgcagctc gggtcgaccg acgtcgtcac
     1921 ggcggcgccc atgaccgccg gcgccgcgtt cacgccgacc cactacaaca tccgcaagct
     1981 cggcggcgcc ggccggttca ccttcaaggg cttcgagggc aagctgcggc tcggggtgga
     2041 gagcggcttc gcatccggcg accagtactt caataccccg atcggcgcga ccaaccttgc
     2101 gtacggtagc ccgttcggca acacgatcct gccgaacggc atggtgcagg tcgcgccgaa
     2161 cgactcgacg ctgaagcagt tcgcgttcaa ccgcgactac caggtcgacc tgatcctgtg
     2221 gcgccacctg gtcggtgcgg tcagcaacgc ggcgtacttc aagccgttcc tccagtacga
     2281 catcacccgg tcgatcacgt tcaaggtcgc gaacatcacc tcgatcgcgc tccacaagga
     2341 ggcgacgccg ggcgacggcc gcgtctacgg caccgagttc gacggcgacc tcggctacgc
     2401 cagcaacggc gtgttcgtcg ggatctcgta cggcgtgctg ttcccgttcg gcgcgatgga
     2461 ccacccggat ctcaacccca gcgattccag cccgccctac gggcattcca ccacgtcgcc
     2521 gaacaacctc ggcaccgcct cgaccgcgca cacgatccag tcgcggttcg tgctcgcgtt
     2581 ctagcaggct ggcgccgcgc gttttcgatc cgtcacagct cgccgagcgc ggtgaccaat
     2641 cgatcgacgg gcacgagctc gatggccccg gcggtctgcg ccgcgtgggc cgcgttctgg
     2701 gccggcacga tcgcccggcg gaacccgagg cgcgctgcct cggccagccg ctgatcgcac
     2761 agcggcaccg cgcggatctc gccggcgagc ccgacctcgc cgaacaccac ggtgtgggca
     2821 tcgagcgcgc gaccgcgcgt cgcgtcgcgc gtttcgattc cttcacagct cgccgagcgc
     2881 cgtgaccagc cgatcgacgg gcacgagctc gatcgccccg ccgccctgcg ccgcgtgggc
     2941 cgcgttctgg gccggcacga tcgcccggcg gaaccccagg c
//
LOCUS       DASVOR010000030         8020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_380207,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000030 DASVOR010000000
VERSION     DASVOR010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380207"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..667
                     /locus_tag="VF516_00650"
     CDS             419..667
                     /locus_tag="VF516_00650"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX8106196.1"
                     /translation="MADPSKVLAEALDLPVRERLRIASALLRSVDPEEDDDDAEAWVA
                     ELDRRSASIENGTAQLIDAAAAHAHVREVLRARRAARR"
     gene            complement(860..1477)
                     /locus_tag="VF516_00655"
     CDS             complement(860..1477)
                     /locus_tag="VF516_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106197.1"
                     /translation="MHPSASHAAIDVPALRFAILALALASWCAAGPSTRAAGPDIRPV
                     VIRIDETTTFEHFVEETSARLGEPCLFVWGDAGELPGARPGAIAWSDGQIDGWFLHDD
                     FGGVLRDPRVKDFVFVIYDDGTLWGSHPSGWRRWPLDIPDQPSEDRWGLLAIVVPEAD
                     PGALILPTFRAVRWLHRIAQFSAFEASRARHSCSRSHPAPHPWPR"
     gene            2072..2650
                     /locus_tag="VF516_00660"
     CDS             2072..2650
                     /locus_tag="VF516_00660"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8106198.1"
                     /translation="MGRVAERSQLTAAEYLAWEREQPVKHEFFHGEVFAMAGGTPRHN
                     ALGVSMGAELRAALRSRGCSVLSSDQRLAFPPETRYVYPDVTVICGPPVFQRGTDDVI
                     TNPAILVEVLSTSTEEYDRGMKWAGYQLIGSLTDYLLVAQAEARIEHYRRNADGSWTY
                     RTAGPGEHVVLTGGGELAVDAIYAGVFDLPGE"
     gene            complement(2812..3039)
                     /locus_tag="VF516_00665"
     CDS             complement(2812..3039)
                     /locus_tag="VF516_00665"
                     /inference="COORDINATES: protein motif:HMM:NF037832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106199.1"
                     /translation="MVVSGDLDFSNALRFPPGTHPGIVVARLPDAMPPSDMAARIVAA
                     IAAMGADLNGAITIVEATRMRVFGGGDPNGT"
     gene            complement(3192..3416)
                     /locus_tag="VF516_00670"
     CDS             complement(3192..3416)
                     /locus_tag="VF516_00670"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX8106200.1"
                     /translation="MTEIVPGITADSARAFGKPVIAGTRVPAATVLGLLAAGRPEAEI
                     LAEYDLTTEQLRAVFRYAAWLASQQSLRAS"
     gene            complement(3564..3755)
                     /locus_tag="VF516_00675"
     CDS             complement(3564..3755)
                     /locus_tag="VF516_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106201.1"
                     /translation="MAQAVAHLDASPSVPPEMIARIVEVLHPEQVWLFGMRPSRTSTL
                     PRCGSGSGTYGSGASRCSR"
     gene            complement(4304..4801)
                     /locus_tag="VF516_00680"
     CDS             complement(4304..4801)
                     /locus_tag="VF516_00680"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8106202.1"
                     /translation="MVELGMFEDERVELLRGVLIKMSPQLAAHASTVARLNQLLMAAL
                     RGRFGLRIQSPLALSDDSEPEPDVAVVALGNYDVEHPHTALLVVEVSDTTLRRDRGKA
                     SLYALAGIPEYWIVNLGARTVEVYSSPDGDRYADARTMRAGDTLRPALLADGALAVAE
                     ILPGA"
     gene            complement(4887..5108)
                     /locus_tag="VF516_00685"
     CDS             complement(4887..5108)
                     /locus_tag="VF516_00685"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8106203.1"
                     /translation="MAQAVAHLDASPSVPPEMIARIVEVLHPEQVWLFGSRARGEARA
                     DSDWDFMAILADDADASCSRLIAREPRSW"
     gene            complement(5289..5546)
                     /locus_tag="VF516_00690"
     CDS             complement(5289..5546)
                     /locus_tag="VF516_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106204.1"
                     /translation="MVTHTSKGDVVSGYTRAQWPALCAEVAKLRVSTLDGEILPLATV
                     LATAELRAEKRWQKVVTPMGLEPWRRVTTRRMKTATSHDLA"
     gene            complement(5759..6781)
                     /locus_tag="VF516_00695"
     CDS             complement(5759..6781)
                     /locus_tag="VF516_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106205.1"
                     /translation="MSEQPGREDVPQGPHVGCEQSSLAIAAALVGHVIALLAAVTDSV
                     ALNVVVVAGFGGSVIAIAALAALAPAELRTRRAFTALIGAHGLAAAIMLSGLALSVPP
                     ALPLATLAAAAALGGACLAIVRFASMADRPPRPLAIAPRVAAIALVIQMILQRWAASA
                     SARAAPLRAAGFLALSVLGIVLLASMATIALRLRRRWLIAGLLAWTVGWVASMADLSA
                     LILWSLGGTFPVGRHQLGVLDAEFVQLIATLGGLAIGAALVMAIRESRFRHSAMILLA
                     GYAAFGLIAAVGEHRIDLAADFPSIVGLRRDRDLADAITDVALAGALWLYWRRVAANR
                     LPASTW"
     gene            complement(7026..>8020)
                     /locus_tag="VF516_00700"
     CDS             complement(7026..>8020)
                     /locus_tag="VF516_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106206.1"
                     /translation="PPAPPAPPPKTLAAIDVFGAKHVSNEEIIATAGLVVGSPVEFGS
                     EEFGEQIEAATKRLKERYRFAFVALSPISYFGSSPDAGKVFITIDVVEAEDAGRLKFA
                     PEPSKDIPDPDGLVAAWLDYEKTVWPLQSRGALKPPYTCKGGMHCALGFNHPDLEHRE
                     DVFIEKVPRHVAELTAVLHSDRAAERRAAAAFLLPYAKDRKQVINALLPSIDDPSSGV
                     RNNAMRVLVMIQQSADTVVVPLKPVLRAMRFPTTTDRNKAAYLLAGIAKHAPAADRAR
                     IARELGDVLVVMAAMKQPNNRDPAIDILKAISGEDYGTDAAAWRAWLDRRRARR"
BASE COUNT         1340 a         2791 c         2689 g         1200 t
ORIGIN      
        1 ccgcatcgca tctcggggta gcgtcgggcc attggggtca tcgagatcgc gccggccgag
       61 ctcaagaggt gcgtgctccc gttgcttgcc gtgactgagc aggctaagtg gacgccgatc
      121 ggcacgtgct tcatcgtcgc agtcctagat gcgctgaacg cggttgccgt cactgcgacg
      181 cacaatctcc gctacgctgc gcggatctct ccgcgctacc gcgtgcctac tccgggtgtg
      241 ttcgctcgtg aacccgacgc cgggtggcca gaatttccgg aatcgatcag gtcagaattt
      301 ctggaacccg cacctggtcg ccgacagtcg cgcttgcagc acctgcagtt ccttcgcgag
      361 cccctcgcct gctcgtcgcg tctgcgacag gagcgacctg ttacgctaga ctcggttcat
      421 ggccgatcca agcaaggtac tcgccgaggc gctggacctc ccggtccggg agcgcctccg
      481 gatcgccagc gctctgctgc gtagcgtcga tcccgaggag gacgacgacg acgccgaggc
      541 atgggtggcc gagttggacc gacgatcggc gtcgatcgag aacggcacgg cccaactcat
      601 cgatgccgct gctgcccatg cccacgtgcg tgaggtgctc cgagcacgac gcgcagctcg
      661 ccggtgaacg ttcggtacct ttccgaggcg ctcgccgagt acgaagcggc agcgaagtgg
      721 tacgacgaca gccgtgatgg ctcgtagccc gggcgccatc tgcgttggct ccccgcgtct
      781 acctggtagc aagcagcagg atctctccgt cgagggtcga cacacgcagc ttggcgacct
      841 cggcgcacag agccggccac taccgtggcc atggatgcgg tgcaggatgc gaccgtgagc
      901 acgagtgccg tgcgcgggaa gcctcgaagg ccgagaactg ggcgatccgg tgcagccagc
      961 ggacggcgcg gaaggtcggc aagatgagag ccccagggtc ggcctcggga acgacgatgg
     1021 cgagcaggcc ccagcggtcc tcggagggct ggtcagggat gtcgaggggc cagcgcctcc
     1081 aaccggaggg atgggagccc cagagcgtgc cgtcgtcgta gatcacaaac acgaagtcct
     1141 tgacccgcgg gtcgcgcaaa acgccgccga aatcgtcgtg caggaaccag ccgtcgatct
     1201 gaccgtcgga ccaggcgatc gcgccgggcc gggcgccggg gagctcgccg gcgtcgcccc
     1261 agacaaacag gcatggctcg ccgaggcgtg cggaggtttc ttcaacgaag tgctcgaacg
     1321 tggtggtctc gtcgatccgg atgacgacgg gacggatatc ggggccggcc gcgcgggtac
     1381 tggggccagc ggcgcaccac gaggcgagcg cgagcgcgag gatggcgaac cgcagagccg
     1441 gtacgtcgat cgcggcatgg ctcgcggagg ggtgcatcgc gggcacggta gcaccaaacg
     1501 cagcgcgccc ggctcgcaaa ccgccagacc tgttcagggt gcagcacctc gacgatgcgc
     1561 gcgatcagat ccaccggaac gcgcagtgac cgagtgactg gggacacgcg ttgatcatgc
     1621 ggggtctggc tgacgctcga ccgccctgcc ccaccgcgct ccgtggcagg tcgcgtctgg
     1681 cgagaccgcc ggttcgtctg gcttcgtcat ggtgtcatcg tttgctaccg ccgcaccgcg
     1741 cgtcgagctg gcgccgcacc tcgggcgtcc acctggtcgc cgttgatcag ctccgcgcgg
     1801 ccgtcagcca cttcgcgggc tcggcgatcg agctcgccag cccacgcggc ttcccactca
     1861 gcgctggtca cgttgtctcc gtcgtcgggc tcgaggctgc gcaggagctg cgtgaccagc
     1921 ttccagcgtt cctcctcgag gagatcgacg atgcaggaca gccgggcgcg tcgattcggg
     1981 cagcatggga acgcgttccc agcgcgatgc tctcaccggc tgctcgatcc cgcggccagg
     2041 ctcgctatcg agcgcgtggt acgctggccc catgggccgt gtcgccgagc gctcccagct
     2101 gaccgccgcc gagtacctcg cgtgggaacg tgagcagccg gtgaagcacg agttcttcca
     2161 cggcgaggtc ttcgccatgg ctggcggaac gcccaggcac aacgcgctcg gggtcagcat
     2221 gggcgccgag ctgcgtgctg ccctgcggtc gcgcggttgc tcggtcctgt cctccgatca
     2281 gcggctcgcc ttccctcccg agacgcgcta cgtgtacccg gacgtcacgg tgatctgcgg
     2341 cccccccgtg ttccagcgtg ggacggacga cgtgatcacg aacccggcga tcctggtcga
     2401 ggtgctgtcg acctcgaccg aggagtacga ccgcggcatg aagtgggccg gataccagct
     2461 catcggctcg ctgaccgact acctgctcgt cgcccaggcg gaagcccgga tcgagcacta
     2521 ccgccgcaac gccgacggct cgtggaccta ccgcaccgcc ggacccggcg agcacgtcgt
     2581 gctgaccgga ggcggcgagc ttgcggtcga cgcgatctac gctggcgtct tcgacctgcc
     2641 cggcgagtga tcacaggtcg ctacgccgct ccgtccggag gcgactgtcg cccaaaaatc
     2701 cgagatgagc agggaccagc cggggtgaac cgggatcaga cagccgggtg actacgcgag
     2761 atcgtgcgaa gtcgcggttt cattcgccgc gtcgtcacct ggcgccaggg ttcaagtccc
     2821 attggggtca ccaccgccga agacgcgcat gcgcgtcgct tcgacgatcg tgatcgcgcc
     2881 gttgaggtca gcgcccatgg cggcgatggc cgcaacgatc cgagcggcca tgtcggacgg
     2941 aggcatcgcg tccggcagac gagccacgac gatgcccggg tgcgtacccg gcgggaagcg
     3001 caacgcgttc gagaaatcca ggtcgccgga cacgacgatc gctgagtctc tcgcgcccgc
     3061 gcggcgatcg catcatcggt ggcgcctcgg agaccaacgt cacggacatc ggcgacatcg
     3121 tagccggctg caacgagcgc tctcgtcacc gcacggggca gtgattcatc gacgagaaat
     3181 gcagccacct gtcagctcgc acgaaggctc tgctgtgacg cgagccacgc tgcatatcgg
     3241 aagacagccc ggagctgctc cgtcgtgagg tcgtactcgg cgaggatctc ggcctccggg
     3301 cgaccggcgg caagaagccc gagcacggtc gccgcaggca cacgggttcc ggcgatcacc
     3361 ggcttgccga aggcacgcgc ggaatcggcg gtgatgccag gaacgatctc ggtcacggaa
     3421 gaagtgtacc tgacctcgcg ctccacaggt acccgcagat cgggtgcggg aggctcagct
     3481 gccatagacg acaacgcccg tcgctgcgac gatctccgcg agcgtaccca gcgagcgctt
     3541 ccatccgtcg aattcgctgc gcgtcatcgt gaacacctcg acgcgccgga gccgtaggtc
     3601 ccggagccgc tgccacaccg aggcaaggtc gaagtcctgc tcgggcgcat cccgaacagc
     3661 cagacctgct cggggtgaag cacctcgacg atgcgggcga tcatctccgg cggaacgctc
     3721 ggcgacgcat cgaggtgggc aacagcctgg gccacgcgcc gaccctaccg gattcgctgg
     3781 cgctcgcctg ccccgccaca cctgcctgcc ccggccgcgc ttgtagcagc tcacggccgg
     3841 tcggacccga gctgcttccg ggaggcaacg gttgcccaaa catccgggat cggccgggac
     3901 cagccgggat gaaccgggac caggcgacca ggtaactacg cgagatcgtg cgaattcgct
     3961 gttttcattc gccgcgtcgt caacccggca ccagggttca agtcccattg gggtcaccgt
     4021 caccgaagac gcgcacgcac atcgcttcgg cgatcgcctt gcgtcagcta ccatggtctg
     4081 atcgcatcgc gctttcgtcc tcgctcagga gccggcggcc ttcttcgctt cggcgacgcc
     4141 cggcggagcg cgtcgttgac agcctcggcg gagccgaagt gcgaccagat cgcgctgatc
     4201 ggcggcggca cgttcgccgt ggatgcgatc tacgccggca tgttcgagcc gtcgggcgag
     4261 tgacgcggcg aggcgccagc acggccggtc gatctggcga cgctcaagcg cccggcaaga
     4321 tctcggcgac cgcgagcgcc ccgtcggcga gcagcgctgg cctcaacgtg tctcccgcgc
     4381 gcatcgtacg ggcatcggcg taccggtcgc cgtcgggcga cgaatacacc tcgacggtgc
     4441 gagcaccgag gttcacgatc cagtactcgg ggatccccgc gagtgcatac agcgaagcct
     4501 tgccgcgatc cctccgcagc gtcgtatccg agacctcgac gacgaggagc gcggtgtggg
     4561 gatgctcgac gtcatagttc ccgagcgcca cgaccgcgac gtctggctcg ggctccgaat
     4621 cgtccgaaag tgccagcggc gactggatgc gaaggccgaa gcggccccgc agcgcggcca
     4681 tcagcagctg gttgagcctg gcgaccgtcg aggcatgcgc cgcgagctgt ggtgacatct
     4741 tgatcagtac cccccggagt agctcgacgc gttcgtcctc gaacatcccg agctcgacca
     4801 accgatcgta ttccgcgcgc ttgagcgacc gtaccgattc cggctcgatc gacgcaaagg
     4861 cttcggagcc agccatgtga tcctgtctac cacgaccgcg gttcgcgagc gatcagcctg
     4921 ctacaggacg cgtccgcgtc gtcggccagg atcgccatga agtcccagtc gctgtccgcg
     4981 cgcgcctcac cgcgcgcccg gctcccgaac agccagacct gctcggggtg aagcacctcg
     5041 acgatgcggg cgatcatctc cggcggaacg ctcggcgacg catcgaggtg ggcaacagcc
     5101 tgggccacgc gccgacccta ccggattcac tggcgctcgc ccgccccccg cccgcctacc
     5161 tgccccggcc acttgtagca gctcgcgccg ggcgcgaccg cggccaagat ccgggaggca
     5221 accgttgccc aagaatccga gatcagccgg gaccaaccgg gaggaaccgg gacacggcaa
     5281 ccaggtaact acgcaagatc gtgcgaagtc gctgttttca ttcgccgcgt cgtcacccgg
     5341 cgccagggtt caagtcccat tggggtcact actttttgcc agcgcttctc cgcacgtagc
     5401 tctgctgtag caagcactgt agcaagcggc aggatctctc cgtcgagggt cgacacgcgc
     5461 agcttggcga cctcggcgca cagcgccggc cactgcgccc gcgtgtagcc gctgaccaca
     5521 tcgcccttcg aggtgtgcgt gaccacgcgc agcagatccc ggtgcgcgcc gtgctcctgg
     5581 cacgtggtga tgaaccacga ccgcaggtca tgcccgcccc accagcgccg cgatcggcga
     5641 cccgcgcgag ctcctgcgcc gccgagctgg tggcgccggt gccggtccta cggccgcatc
     5701 gaggtcgccg ccgccgcgct ggtgacgtcc gcaccgcaac cgccgcgcgt cagatgcgtt
     5761 accaggtcga agcaggcaag cggttggcgg cgacccgacg ccaatacagc cacagcgcgc
     5821 cggccagtgc gacgtcagtg atcgcatcgg cgaggtctcg atccctgcgc agccctacga
     5881 tgctcggaaa gtccgcggcg agatcgatcc ggtgctcgcc cacggcggcg atcaggccga
     5941 aggcggcata gccggcgagc aatatcatgg ccgaatggcg aaaccgcgac tcgcggatcg
     6001 ccatcaccag tgccgctccg atcgccaggc cgcccagcgt ggcgatgagc tgcacgaact
     6061 cggcgtcgag aacacccagc tgatgccgcc ccaccgggaa ggtccctccc agcgaccaga
     6121 ggatcagcgc gctgaggtct gccatgctgg ccacccatcc caccgtccac gccagaagcc
     6181 ccgcgatcag ccagcggcgg cgcagccgca gcgcaatggt cgccatgctg gcgagcagga
     6241 cgataccgag caccgacagt gcaaggaagc cggcggcacg gagcggcgcg gcccgtgcgc
     6301 tggcggacgc tgcccagcgc tgcaggatca tctggatcac gagcgcgatc gcagcgacgc
     6361 gcggcgcgat ggcgagcggc ctcggcgggc gatccgccat tgacgcgaat cggacgatgg
     6421 cgaggcacgc gcccccgagc gcagcggcag ccgcgagtgt ggccagcggc agtgccggcg
     6481 ggacgctcag tgcgagcccg gacagcatga tcgccgcggc cagaccatgc gcgccgatca
     6541 gcgcggtgaa ggcgcggcgt gttcgaagct ccgccggcgc gagcgctgcc agcgctgcga
     6601 tcgcgatgac agagcctccg aaccccgcga cgacgacgac gttcagggcg accgaatcgg
     6661 tcacggcggc gagcagggcg atgacatggc cgacgagcgc ggccgcgatg gctaggcttg
     6721 actgctcgca acccacatgt ggaccctgcg gcacatcctc gcgcccgggc tgctcgctca
     6781 tggaacgagg ctatcagata gagacctgct cggcagcgct gcgccgatgg ccatgctcgt
     6841 cccaccatgg gcgcgcacga cgtatcatcg tcaccgtcaa gaacggcgct cgcaaaccgg
     6901 tcacggcgag ctgcccaagc tccctagtga agtcgggcgg cctcggcctc gatcgatccg
     6961 atcgcgtcgc gcaggggcgt cgccgagtac gcgacgaaaa tgtcgatcct gacgcggcga
     7021 tgccgctacc tgcgggcccg ccttcgatcg agccacgctc gccaggccgc agcgtcggtg
     7081 ccgtagtcct cgccggagat cgccttcagg atgtcgatcg ccggatcgcg gttattgggc
     7141 tgcttcatcg cggccatcac caccagcacg tcgccgagct cgcgcgcgat ccgggcgcga
     7201 tcggccgcgg gcgcatgctt ggcgatcccg gcgagcaggt acgcagcctt gttgcgatcg
     7261 gtcgtggtcg gaaagcgcat ggcgcgaagc accggcttca gcggcacgac caccgtgtcc
     7321 gcactctgct ggatcatgac cagcacccgc atcgcgttgt tgcgcacccc cgagctggga
     7381 tcgtcgatcg acggcagcag cgcgttgatg acctgcttgc gatccttggc gtagggcagc
     7441 aagaacgccg ccgccgcccg ccgctcggcg gctcgatcgc tgtgcagcac cgccgtcagc
     7501 tcggcgacgt ggcggggcac cttctcgatg aatacatcct cgcggtgctc gaggtcaggg
     7561 tgattgaacc cgagcgcgca gtgcatgcca cctttgcacg tgtacggcgg cttgagcgct
     7621 ccgcggctct gcaggggcca caccgtcttt tcgtagtcga gccacgcggc gaccagcccg
     7681 tccggatccg ggatgtcctt cgagggctcg ggcgcgaact tcaggcgacc ggcgtcctcg
     7741 gcctcgacca cgtcgatcgt gatgaacacc ttcccagcat ccgggctgga cccgaagtag
     7801 ctgatcggcg acaaggcgac gaatgcaaat cggtagcgct ccttgagccg cttggtagcg
     7861 gcctcgatct gctcgccgaa ctcctccgac ccgaactcca ccgggcttcc caccacgagc
     7921 cccgccgtgg cgatgatctc ctcgttcgac acgtgcttcg cgccgaatac gtcgatcgct
     7981 gccagcgtct tcgggggcgg agccggcggc gcaggcggtg
//
LOCUS       DASVOR010000031         5553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_409,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000031 DASVOR010000000
VERSION     DASVOR010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1308
                     /locus_tag="VF516_00705"
     CDS             <1..1308
                     /locus_tag="VF516_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106207.1"
                     /translation="TNVAGSKGQWASHPTIIEELQPGSGGYADADAITATYWWLYRYV
                     KWTTTDYYKIRRPSPPDVVNIPDFPSPPGTGESDPGPGPSDDSAWHDFLEILLAIFAW
                     IIYLAQVAAWPATAIVSLITSAGTYPVRQLLYENLELPLYNAWLALHWYLSMTGFNYP
                     MQEEINPGLMTLGVGVGDVWGSVQAALSDPSGGLHMPAAGTEPSGRDRDRTTPHEVVV
                     DPQGWVASAIHQALNNVCGASEAPSEFLRPWKWPDKDNQGDMVPSEQPLSPASPYVAS
                     NDATVLIGGAAGNAAVRTALEHCHNEAETIKQATAHLPHGEHLGDPVDYTGYVIAWLT
                     RDTPGEIANFNLDSDRGYGYLCWDWLRAKSPDMTGTPASFKGHADPHRYRAPIRPGAG
                     WCDQELDHPSPHGANDPVMHDPVNPDKTGPVRIRYIDREDKTP"
     gene            1305..2570
                     /locus_tag="VF516_00710"
     CDS             1305..2570
                     /locus_tag="VF516_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106208.1"
                     /translation="MSEHPPKNPIFDKAPGSPTAPKPDTTEKDRVAKIQELLRQMVSR
                     QHDKGRAQQFLPYVMVRSTVGDHGNRPINVPFWESPDIWTAPGDPSTSPAIPASPGGT
                     VHAGVANTVYAHVWNLGRAPIAGVKVEFYWFNPSLGIDGAHAHLIGQARVDLAPRSSS
                     ACHKLVKCPRAWTPVLENNGHECLVVRVSAIGDSIGSSHPWDSWANRHVAQRNISVLP
                     AGHAFDATLSKLADTRLKGTRLQLLQLGTEGKLAAQLVAPRLALDPRVHTQVLAELRA
                     DNSLVLPSAVPVVLGAGAAPAHPAATAVAPAHPNVASAPVAVAHDALALPRIVPSAVL
                     GAVSIPAVDARAVVAAPGVHPQGHVEVLHSGGSLDALINHTAFLTPALQAKLQALSPP
                     PAGQVQVMRIAQYRGDQLIGGYTIVTTAK"
     gene            2593..3030
                     /locus_tag="VF516_00715"
     CDS             2593..3030
                     /locus_tag="VF516_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106209.1"
                     /translation="MPFANAGSGIFLVSDHSGAKRCSACTEAWQVINGFLTTGAATAF
                     GVKVVVNSSAGGFLVASQLTTGGRLVVLGDSSPADDGTCTGCSASLHDGWDEGSARAF
                     SSTRPSGSPTARARSDRKRSPDRAGTSRAARRFTPASVASRFR"
     gene            complement(3027..4466)
                     /locus_tag="VF516_00720"
     CDS             complement(3027..4466)
                     /locus_tag="VF516_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8106210.1"
                     /translation="MPQPPRSVGAPSAEGIRTFALSPVLLRRVISGRSQPLPSVIQGK
                     VEQALGVDFSDVRIRIGPEPAALGANAFSTGSTIYFAPGRFQPQTLEGQRLIARELVH
                     IHQQRTGRLAPPRDGTCRIVHDPVLESEADQIAALILQPSLTRALVTIRRSASIPLIL
                     SHGCTIAGASVVQRMEDSGMRDRLVVSAESPLDESVQPISLIVDDEKAAAEEQPATNP
                     PGHQYARFDQNPLFPIEMEDWLMFVNQGSLGDCHFLAGVAALVITRPRYLEAMILERR
                     TEDGRVFEVKFKNGKILVDDQLPVSSGALKFASKEVTPNSNHAIFAAILEKAAACRLG
                     SYNALIGGQPADTLKLLASSTKSFIPGKYSKREDWQQHLALLCSKRYPAVLLMDTNYV
                     RFIKGIRESSRQGSHVVAINRIEKRSERFVVTIFDQGKRPGDPRRNLEIGENDLVNSL
                     FKGTFPLEFPEGTEEETRYVLQGTVYLVI"
     gene            complement(4876..>5553)
                     /locus_tag="VF516_00725"
     CDS             complement(4876..>5553)
                     /locus_tag="VF516_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106211.1"
                     /translation="LFAKAGTPGADPGAAGCVQCHGSAGWTVSRLFYRATTQRTDQLA
                     ATAFQPPAAFPAAAVVGALAWSFQTLQIAIQPASSLFDADEATTAIGPNQLACVLRNT
                     GTFGVPGDATATSALELKNDPTKPSFASRSQGRLGYNVPSLYGLSLGAPYLHHGGAHT
                     LAELFDDPKWKQHAEAGSAVWLHQGTADDIAARKRDLINFLLSIDATTAVQDIPAGFD
                     ACPANEL"
BASE COUNT          912 a         1874 c         1866 g          901 t
ORIGIN      
        1 accaacgtgg caggctcgaa gggccaatgg gcgtcccacc cgacgatcat cgaggagctg
       61 cagccgggct cgggcggcta cgccgacgcc gatgcgatca ccgccacgta ctggtggctg
      121 tatcgctacg tgaagtggac cacgacggac tactacaaga tccgccgccc gtccccaccg
      181 gacgtggtca atatccccga cttcccgtcc ccgccgggca ccggcgagtc ggatccgggg
      241 ccgggcccca gcgacgacag cgcatggcac gacttcctgg agatcttgct cgcgatcttc
      301 gcgtggatca tctacctcgc gcaggtggcg gcgtggccgg cgaccgcgat cgtcagcctg
      361 atcacgtcgg cggggacgta tccggtccgc cagctgctct acgagaacct ggagctgccg
      421 ctctacaacg cctggctcgc gctgcactgg tacctgtcga tgaccggctt caactatccg
      481 atgcaggagg agatcaaccc cgggctgatg acgctcgggg tcggcgtcgg cgacgtgtgg
      541 ggcagcgtgc aggccgcgct gagcgatccg agcggcgggc tgcacatgcc ggcggccggg
      601 accgagccgt cagggcggga ccgcgatcgc acgacgccgc acgaggtggt ggtcgacccg
      661 cagggctggg tcgcgtcggc gatccaccag gcgctcaaca acgtgtgcgg cgcgagcgag
      721 gcgccgagcg agtttctgcg gccgtggaag tggcctgaca aggacaacca gggcgacatg
      781 gtcccgtcag agcagccgct gtccccggcc agcccctacg tggcgtcgaa cgacgccacc
      841 gtgctgatcg gaggcgcggc gggcaatgcc gcggtccgga ccgcgctgga gcattgccac
      901 aacgaggccg agacgatcaa gcaggcgacc gcccacctcc cgcacggcga gcacctcggc
      961 gatccggtcg actacaccgg ctacgtgatc gcatggctca ctcgcgacac ccccggcgag
     1021 atcgccaact tcaacctcga ctcggatcgc ggctacggct acctgtgctg ggactggctg
     1081 cgggcgaagt ctccggacat gaccggcacg ccggcgtcgt tcaaaggaca cgccgacccg
     1141 caccgctatc gcgcgccgat ccggcccggc gccggctggt gcgatcagga gctcgaccac
     1201 ccgtcgccgc acggcgcgaa cgatccggtg atgcacgatc ccgtgaaccc ggacaagacc
     1261 gggccggtgc gcattcgcta catcgaccgg gaggacaaga cgccatgagc gagcaccctc
     1321 cgaagaatcc gatcttcgac aaggcgcccg gttcgccgac cgcgccgaag cccgacacga
     1381 ccgagaaaga ccgggtcgcc aagatccagg agctcctccg ccagatggtc agtcggcagc
     1441 acgacaaggg gcgcgcccag cagttcctgc cctacgtgat ggtccggtcg accgtgggcg
     1501 atcacgggaa ccggccgatc aacgtgccgt tctgggagag cccggacatc tggaccgcgc
     1561 cgggcgatcc atcgacctcg ccggcgatcc ccgcgtcgcc cggcggcacg gtccacgcgg
     1621 gcgtggccaa cacggtctat gcccacgtgt ggaacctcgg gcgcgccccg atcgccggcg
     1681 tgaaggtcga gttctactgg ttcaacccat cgctggggat cgacggcgcg cacgcccacc
     1741 tgatcggcca ggcgcgcgtc gatctcgcac cgcgcagctc cagcgcctgc cacaagctgg
     1801 tcaagtgtcc ccgggcctgg acgccggtgc tggagaacaa cggccacgag tgcctcgtcg
     1861 tccgggtctc cgcgatcggc gattcgatcg gcagctcgca cccatgggat tcgtgggcca
     1921 accggcacgt cgcacagcgg aacatcagcg tgctgcccgc cggacatgca ttcgacgcga
     1981 cgctgagcaa gctggccgat acccggctca aggggacgcg gctgcagctc ctgcagctcg
     2041 gaaccgaggg caagctggcg gcccagctcg tggcgccccg gctggcgctc gatccccgcg
     2101 tgcacacgca ggtgctcgcc gagctgcgcg ccgacaatag cctggtcttg ccatccgcgg
     2161 tgccggtggt cctgggtgcg ggagccgcgc cggcgcaccc ggccgcaact gcggtggccc
     2221 cggcgcaccc gaatgtcgcc tcggcaccgg tcgcagtggc gcacgacgcg ctggcgttgc
     2281 cccgcatcgt gccatcggcg gtgctcggcg cggtgtcgat ccccgcggtc gacgcgcgcg
     2341 ccgtggtggc ggcgcccggc gtccacccgc agggacatgt cgaggtcctc cacagcggcg
     2401 gaagcctcga tgcgctgatc aaccacacgg cgttcctgac gccggccctc caggcgaagc
     2461 tccaggcgct gtcgccgccg cccgccggcc aggtgcaagt gatgcggatc gcccagtacc
     2521 gcggcgatca gctgatcggc ggctacacca tcgtcacgac cgcgaagtag acgaccgtca
     2581 tgctccgccc tgatgccgtt cgccaacgcg ggcagcggca tcttcctggt ctccgatcac
     2641 tccggcgcca agcgatgcag cgcctgtacc gaggcgtggc aggtgatcaa cggcttcctc
     2701 accacgggag ccgccaccgc cttcggcgtc aaggtcgtcg tcaactcctc ggccggcggc
     2761 ttcctcgtcg cctcgcagct caccaccggc ggccggctcg tcgtgctcgg cgactccagc
     2821 cccgccgacg acggcacctg caccggctgc agcgcatcgc tccacgacgg ctgggacgag
     2881 ggctccgcgc gcgcgttctc ctcgacgcga ccgtctggct cgcccacggc gagggcacga
     2941 agcgatcgca agcggtctcc tgaccgcgct gggacctcgc gcgcagctcg gaggttcacg
     3001 ccggcgtccg tcgcttcgcg attccgttag atgacaagat agaccgttcc ttggagtacg
     3061 taccgagttt cctcttctgt tccctcgggg aattccagag gaaacgttcc cttgaacaag
     3121 ctgttgacga gatcgttctc accgatctcc aggttgcgcc ttggatctcc ggggcgcttt
     3181 ccctgatcga agatggtgac gacaaatctc tcgctgcgct tttcgatcct gttgatcgcg
     3241 acaacgtgcg acccttgtcg cgaggattcg cggattcctt ttatgaacct cacgtaattc
     3301 gtatccatga gcaagacagc gggatatctc ttgctgcata aaagggcaag atgttgttgc
     3361 caatcttcgc gcttggaata ctttcctggg atgaatgact ttgtgcttga cgcgaggagt
     3421 ttgagcgtat cggcaggttg cccgccgatc agcgcgttgt agctgcctaa ccggcatgcc
     3481 gcagccttct ccagaatggc cgcaaaaatg gcgtggtttg aattcggcgt cacctctttc
     3541 gatgcgaatt tcaaggctcc actgctcacg ggcagctgat catcgacgag tattttgcca
     3601 ttcttgaact tgacttcgaa gacccgaccg tcttccgtcc tccgctccaa gatcattgcc
     3661 tccagatacc ggggtcgcgt gatgaccaag gcggcgacac ctgcaaggaa gtggcagtcg
     3721 ccgagacttc cctgattgac aaacatcagc cagtcctcca tttcgatggg gaacagggga
     3781 ttttgatcga atcgggcgta ctgatggccc ggagggttcg tcgccggctg ctcctccgca
     3841 gccgctttct cgtcatcgac tatcaaactt atcggctgta cagactcgtc aagcggactc
     3901 tcggcgctca caacgagccg atctcgcatg cctgaatctt ccatgcgttg cacgacagat
     3961 gctcccgcga tggtgcaccc gtggctcaga atgagcggga tggacgcact acgccgaata
     4021 gtgaccagag ccctcgtcag cgaaggttgc aggatcagcg cggcgatctg gtcggcttcg
     4081 ctctccagga cgggatcgtg cacaatacgg cacgtcccgt cacggggcgg agcgaggcgg
     4141 ccggtgcgct gctggtggat gtgcaccagc tcgcgcgcga tgaggcgctg gccttcgagc
     4201 gtctggggct ggaatcggcc gggggcgaag tatatcgtcg agccagtgct gaacgcattc
     4261 gccccgagtg ccgccggctc cggtccgatg cggatccgta cgtcggagaa gtcaacgccc
     4321 agcgcttgct ccaccttgcc ctggatgacg gagggcagcg gctgcgatcg tccgctgata
     4381 acgcgccgaa gcaggactgg tgatagcgcg aaggtccgga tcccttcagc agagggcgcg
     4441 ccgaccgatc gaggcggctg cggcacacgt gactgcatgg acctcgggaa ggcgagcagg
     4501 ccggagcatg gcggctgccc caggagcttg cgagaccgcg gctgcgcacc gcctgtgtgc
     4561 gcgtgctcct cagggaagcg gcccgccgga aatgacgcct ggatgacgag cgggtacgtc
     4621 gtttgtgggg ctgattgtac agatcgagaa aacgggggtc tcgcaggtcg agtgaacggc
     4681 ggcatcgcag ctcctgttcg agcgccggcc agcacgacca ccgacgatcc tgggcaaggt
     4741 agttgccgac aatatgagcg tcaactattc atgatcggcg cttcgtattg tcgatcagcc
     4801 ggtcggggtg ccttgccgtt catgcagcgg ttcgggggcc attcacccga ccgcccgcca
     4861 ccgcccgggc tccgctcaga gctcgttcgc cgggcaggcg tcgaagccgg ccgggatgtc
     4921 ctggacggcg gtggtcgcgt cgatcgacag caggaagttg atcaggtcgc gcttgcgggc
     4981 cgcgatgtcg tcggccgtgc cctggtgcag ccacaccgcg ctgccggcct cggcgtgctg
     5041 cttccacttc gggtcgtcga acagctcggc gagcgtgtgc gcgccgccgt ggtggaggta
     5101 cggcgcgccg agcgacaggc cgtagagcga cgggacgttg tagccgaggc ggccctgcga
     5161 gcgcgacgcg aagctgggct tggtcggatc gttcttgagc tcgagcgcgc tggtcgcggt
     5221 ggcgtcgccc gggacgccga acgtgccggt gttgcgcagc acgcaggcca gctggttggg
     5281 tccgatcgcg gtggtcgcct cgtcggcgtc gaacagcgac gacgccggct ggatcgcgat
     5341 ctgcagcgtc tggaagctcc aggccagcgc gccgaccacc gcggcggccg ggaacgcggc
     5401 gggcggctgg aacgcggtgg cggcgagctg atcggtgcgc tgggtggtcg cgcggtagaa
     5461 cagccgcgac accgtccagc ccgcgctgcc gtggcactgg acgcagcccg cggcgccggg
     5521 gtcggcgccc ggggtgccgg ccttggcgaa cag
//
LOCUS       DASVOR010000032         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58745,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000032 DASVOR010000000
VERSION     DASVOR010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58745"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="VF516_00730"
     CDS             <1..666
                     /locus_tag="VF516_00730"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8106212.1"
                     /translation="FRLVHENQQLIASLSAKNQALLEMNRELEAKIHERTHELAAANA
                     RLAQLAVTDGLTGLYNHRHFHERLALEVERSQRNGLPLSLLMLDVDNFKQFNDTFGHP
                     AGDEVLRQLARVLADTRRANDVVARYGGEEFAVILVDTAKFTAAKVAERVRERVYSHD
                     FAETAPRAGRISVSIGVATFPDDGRDAEALVRAADAALYAAKRAGRNRVVLASQALIA
                     TPA"
     gene            737..>1639
                     /locus_tag="VF516_00735"
     CDS             737..>1639
                     /locus_tag="VF516_00735"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX8106213.1"
                     /translation="MSSEDIAVIGAGSYGTCLAVLFGNAGHRVSLYCRGAEAAAAIDA
                     ARENAAYLPGHKLPRAVQVTSDLAAAVRGKRFVFGVTPSHGIRDVLGRAAPALDPDAI
                     VINAAKGLEDGTLKSIDAIYRDVLPPRIAGRATYLSGPTFAGELAAGMPAAIVLAGHD
                     RETTAAAQHALSHLHFRIYTTDDVLGVLIGGALKNVIAIGAGISDGLGYGSNSRVALI
                     TRGLAEITRMGVALGAHAQTFAGLSGMGDLVLTCSGDASRNRRVGLALGRGRSMSEIL
                     GEMTQVAEGVKTAKVAQQLAGKLGV"
BASE COUNT          234 a          650 c          545 g          210 t
ORIGIN      
        1 ttccgcctgg tccacgagaa ccagcagctg atcgcgtcgc tgtcggccaa gaaccaggcg
       61 ctgctcgaga tgaatcgcga gctcgaggcc aagatccacg agcgcaccca cgagctcgcc
      121 gcggccaacg cccggctcgc ccagctcgcg gtcaccgacg ggctgaccgg cctgtacaac
      181 caccgccact tccacgagcg gctcgcgctc gaggtcgagc gcagccagcg caacggcctg
      241 ccgctgtcgc tgctcatgct cgacgtcgac aacttcaagc agttcaacga cacgttcggc
      301 caccccgccg gcgatgaggt cctgcgccag ctcgcccgcg tgctcgccga cacccggcgc
      361 gccaacgacg tcgtcgcgcg ctacggcggc gaggagttcg ccgtgatcct ggtcgacacc
      421 gccaagttca ccgcggccaa ggtcgccgag cgggtccgcg agcgggtcta cagccacgac
      481 tttgccgaga ccgcgccgcg cgccggccgg atcagcgtat cgatcggcgt ggcgaccttc
      541 cccgacgacg gccgcgacgc cgaggcgctg gtccgcgccg ccgacgccgc gctgtacgcc
      601 gccaagcgcg ccggccgcaa ccgcgtcgtg ctggcgagcc aggcgctgat cgcgaccccc
      661 gcgtagcggc cgccgccggg cgccggcgcg cggcgggtgg tggatccgcc gatcggccat
      721 gatacggtgt cgatacatga gcagtgaaga catcgcggtg atcggcgccg ggagctacgg
      781 cacctgtctc gccgtgctgt tcggcaacgc gggccaccgc gtcagcctgt actgccgcgg
      841 cgccgaggcc gccgccgcga tcgatgccgc gcgcgagaac gccgcgtacc tccccggcca
      901 caagctgccc cgggccgtgc aggtcaccag cgacctcgcc gccgcggtgc gcggcaagcg
      961 cttcgtgttc ggcgtgaccc cgtcgcacgg catccgcgac gtgctcggcc gcgccgcccc
     1021 cgcgctcgac cccgacgcga tcgtgatcaa cgcggccaag ggcctcgagg acggcacgct
     1081 caagtcgatc gacgcgatct accgcgacgt cctgccgccg cggatcgccg gccgcgcgac
     1141 ctacctgtcg ggcccgacct tcgccggcga gctcgcggcc ggcatgccgg cggccatcgt
     1201 cctcgccggt cacgaccgcg agaccaccgc tgccgcccag cacgcgctgt cgcatctcca
     1261 cttccgcatc tacaccaccg acgacgtgct cggcgtcctg atcggcggcg cgctcaagaa
     1321 cgtcatcgcg atcggcgccg ggatctccga cggcctgggc tacggctcca acagccgggt
     1381 cgcgctgatc acgcgcggcc tggccgagat cacccggatg ggcgtcgcgc tcggcgccca
     1441 cgcccagacc ttcgccggcc tgtccggcat gggcgacctc gtcctcacct gctcgggcga
     1501 cgcctcgcgc aaccgccgcg tcggtctcgc gctcggccgt ggccggtcga tgtccgagat
     1561 cctgggcgag atgacccagg tcgccgaggg cgtcaagacc gccaaggtcg cccagcagct
     1621 cgccggaaag ctcggggtc
//
LOCUS       DASVOR010000033         5981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_526507,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000033 DASVOR010000000
VERSION     DASVOR010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5981
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526507"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VF516_00740"
     CDS             <1..276
                     /locus_tag="VF516_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8106214.1"
                     /translation="GRLYDPTADADRAIVDAVGGIARERGVSRAQVAMAWLLQKPGVT
                     SPIVGAVKPHHLTDAAAALALKLTDAEIRRLEEPYRPHAVAGISVGR"
     gene            complement(300..3428)
                     /locus_tag="VF516_00745"
     CDS             complement(300..3428)
                     /locus_tag="VF516_00745"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106215.1"
                     /translation="MTGSVRSSASCPSESTVAGFLERRLSIEQRAELEAHVDQCATCR
                     RLVIEMSPGLQLLSAVAEAHTTHPAYPTGDCESLAKGAVVGRFVVLDLLGRGGMGVVY
                     LAYDPELDRKVALKLLRRGARGVGGMRTHLLVEAQVMARINHANVLAIYDVGNYREQV
                     FAAMELVEGTTLRRWMAERPRGWREVIDVFLLAGEGLAAAHAAGVVHRDFKPENLLLG
                     HDGRVKVTDFGLAAEPLSATGQVAGTPGYLAVEGLVSGCVDQRSDQFSFCVALHEALH
                     GRRPFSAHAPDELIAEVRRGPTFQGRCRRVPARLDHVVRCGLALDPDRRYASMRELLA
                     ELRRVAAVHRMRRRVAVAVGALALAIGATAIVMDTRATPRPPCAGAEAKLSGVWDLGR
                     REAVRSAFLRTAVASAPMAFERAVAALDRYAAAWAGIRAEACEATEVRREQPAALLEL
                     RMACLDRALIQLRALSGALVAADAETVRRAESAAMALPALDACSDPDALRADVSPPLA
                     TTARAGLEPVAGARDEPKPRRIDSDDDPLSSRPDFRLPFGCGEAWQITGKLHHRHAGA
                     EADFTLPGDQPSAGFAVFASAPGWVSRLAPDNGEVDIGHGDGWFTTYQHMTDITVSLH
                     QYVGRGQVIGRIGNVNVRNALGGPGPAHLHYAQVYQPGVANAEFEHTSGDDRFPLELE
                     HETFDPSTTGAQVRTSTNNCSGGGTPGRAVQYDVPSSPAVLSRSRATVEVVARSSTDH
                     ALFERWYDGGWNSAPLPHTIAGQPAVAVFNGELHVLARKSDGALFDFRYNPFTGWKTT
                     YLDGKVSGDPDVVVYGWNRNLHVAARGADGFLYQWWTGLGGEWSRAMRVGNVRVVGTP
                     ALVSHYDAFYIVARAGDGSVWSWEADRRGLWTEWQLHGAAIDDPDAAVDPWSGLVNIV
                     ARGPDSRIYRWQSKDSDRVPSHAGEGWSDPELVDAERVVTGAPAATIYHRALHIVARG
                     PDRAVHHWWKTQTWQWEATGGAYSGNPGMFTFGDQLQTVGRGTGGTLYSIWYDPVTGL
                     WNLENQDAAVTD"
     gene            3584..4411
                     /locus_tag="VF516_00750"
     CDS             3584..4411
                     /locus_tag="VF516_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8106216.1"
                     /translation="MTAEIDQEALCALYSLGRAVWPEIDLDIATFSTLAGPRMSTVRL
                     DTLRAGDLYLAAACAAGVERAIAALEQHYLSGIASALVRRGYDAAAVADAVQAVRVRF
                     LVGDAGRAPRISEYDGRGSLATWIQVAAHRIAISAHRRHHRETADEADVVAAERSPEL
                     ALLGQRFGAEFEIAFRSAFEALTARERTLLRYQVIDRIGIDQIAAIHGIHRATAARWI
                     AHAREALVEGVRRALQDRLGVDHEELVSLLRVVHSQLELSLRLLWTPSPDHPERNAG"
     gene            complement(4598..5122)
                     /locus_tag="VF516_00755"
     CDS             complement(4598..5122)
                     /locus_tag="VF516_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106217.1"
                     /translation="MKSGKNRTSCSVVLVSALASLLVACVAPGDGGMEPQGGPEGSIP
                     QPAPDGTAPTALDQGKGAGSQFATAPSISPATSFQDIASNGTYIVSPGNFYAVVANPV
                     TGGWRVFALFTCTTYSVNFWNGTGFYFDDQTGNPGTIVYDQNHNPLNPPGTIFPGGGQ
                     QGINWNPVWFIKNC"
     gene            complement(5512..5886)
                     /locus_tag="VF516_00760"
     CDS             complement(5512..5886)
                     /locus_tag="VF516_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106218.1"
                     /translation="MLGADGGLCAVVQVAGTAHAQIGALLAEPKLLIYGAKTGTTDSL
                     ADIAHKDAACRAWNDRHPPAAQLTCGKTPPDDSLFVIAFGVVTARGTVPITLGVQLQR
                     AGKGAAAHVTPEIVHAIASYLQ"
BASE COUNT          872 a         2113 c         2107 g          889 t
ORIGIN      
        1 ggccggctgt acgatccgac cgccgacgcc gaccgcgcga tcgtcgacgc ggtcggcggg
       61 atcgcccgcg agcgcggcgt gtcgcgcgcg caggtcgcga tggcgtggct gctgcagaag
      121 ccgggcgtga cctcgccgat cgtcggcgcg gtgaagccgc accacctgac cgacgccgcg
      181 gcggcgctcg cgctgaagct gaccgacgcc gagatccgcc ggctcgagga gccgtaccgg
      241 ccgcatgccg tggcgggtat ctcggtcggc cgctgacgac ggctgccgct gccaccgggt
      301 cagtccgtga ccgccgcgtc ttgattctcc aggttccaca gcccggtcac cggatcgtac
      361 cagatcgagt acagggtgcc gcccgtcccg cgcccgaccg tctggagctg atcgccgaac
      421 gtgaacatgc ccgggttgcc gctgtaggcg ccgccggtgg cctcccattg ccacgtctgg
      481 gtcttccacc agtgatggac ggcgcggtcc gggccccgcg cgacgatgtg caacgcgcgg
      541 tggtagatgg tcgccgccgg cgcgccggtc acgacccgct cggcatcgac gagctcggga
      601 tcgctccagc cctcgccggc atggctcggg acccggtcgg agtccttcga ctgccagcgg
      661 tagatccggc tgtcggggcc gcgcgccacg atgttcacga gtccggacca cggatcgacc
      721 gcggcgtccg ggtcgtcgat cgcggcaccg tggagctgcc actcggtcca caggccgcgc
      781 cggtcggcct cccagctcca taccgagccg tcgccggcac gagcaacgat gtagaacgca
      841 tcgtagtggc tgaccagcgc aggtgtgccg accacccgga cattgccgac gcgcatggcg
      901 cgcgaccact cgccgccgag cccggtccac cactgataca ggaagccatc ggcgccccgg
      961 gctgccacgt gcagattcct gttccagcca tacacgacga cgtcaggatc tcccgacacc
     1021 ttgccgtcga gataggtcgt cttccatccg gtgaacgggt tgtaccggaa gtcaaagagc
     1081 gcgccgtcgg attttcgcgc gaggacgtgc agctcgccat tgaacacggc cacggccggc
     1141 tgccccgcga tcgtgtgcgg cagcggtgcg ctgttccatc cgccgtcgta ccagcgctcg
     1201 aacagcgcgt gatccgtgga gctgcgcgcc acgacctcga cggtggctcg actgcgcgac
     1261 agcacggcgg gcgagctcgg cacgtcgtac tgcacggcac ggccgggtgt cccgccgccg
     1321 ctgcaattgt tggtgctggt ccggacctgc gcgccggtgg tgctcgggtc gaaggtctcg
     1381 tgctcgagct cgagcgggaa gcggtcatcg ccggacgtgt gctcgaactc ggcgttcgcg
     1441 acacccggct ggtacacctg cgcgtagtgc aggtgagccg gtcccgggcc acccagcgca
     1501 ttcctcacgt tgacgttgcc gatcctcccg atcacctgac cgcgtccgac atactgatgg
     1561 agcgatacgg tgatatcggt catgtgctgg tacgtggtga accagccgtc gccatgaccg
     1621 atatcgacct cgccgttgtc cggagccagc cgcgataccc agccgggggc ggaggcgaac
     1681 accgcgaagc ctgccgaggg ctggtcgcca ggcagcgtga agtcggcttc ggctccggcg
     1741 tgtcggtggt gcagcttgcc ggtgatctgc caggcctcgc cgcagccgaa cggtagccgg
     1801 aagtccggcc gggagctcag cgggtcgtcg tcgctgtcga tgcgccgcgg cttcggctcg
     1861 tcgcgtgcgc ccgcgactgg ctcgagcccg gcgcgcgcgg tcgtcgcgag cggtggcgac
     1921 acgtccgcgc gcagcgcatc ggggtcgctg cacgcgtcga gcgcggggag cgccatcgcg
     1981 gcggactccg ccctgcgtac ggtctcggca tccgccgcga ccagtgcgcc gctgagcgcg
     2041 cgaagctgga tcaaggcgcg atcgagacac gccattcgta gctccagcag cgccgccggc
     2101 tgctcgcgcc ggacctcggt ggcctcgcag gcctcggcgc ggatccccgc ccacgccgct
     2161 gcatagcgat cgagcgccgc gaccgcgcgc tcgaacgcca tcggcgccga cgcgacagcg
     2221 gtccggagga atgcggatcg gacggcctcg cgccgcccca ggtcccagac tccggacagc
     2281 ttggcctctg cccccgcgca cggcggccgc ggcgtggccc gggtatccat cacgatggct
     2341 gtggcgccga tcgcgagcgc cagcgccccg accgccacag ccacgcgccg gcgcatccgg
     2401 tgcaccgcgg ccacccgccg cagctcggcg agcaactcgc gcatcgacgc gtagcggcgg
     2461 tccggatcga gcgcgaggcc gcagcgcacc acgtggtcca gccgcgccgg cacacgtctg
     2521 caccggccct ggaacgtcgg cccgcggcgg acctcggcga tcagctcgtc gggcgcgtgc
     2581 gccgagaacg ggcggcggcc atggagcgcc tcgtgcagcg cgacgcagaa gctgaactga
     2641 tcgctgcgct ggtcgacgca gccggacacg agcccctcca ccgccaggta cccgggcgtg
     2701 ccggccacct ggccggtcgc cgacaacggc tctgcggcga gcccgaaatc ggtgaccttg
     2761 acccggccgt cgtggcccaa gaggagattc tcgggcttga agtcgcggtg gacgacgccg
     2821 gcggcgtgcg cggcggcgag gccctcaccg gcgagcagga acacgtcgat gacctctcgc
     2881 cagccgcgag gccgctccgc catccaccgc cgcagcgtgg tgccctcgac gagctccatc
     2941 gcggcaaaca cctgctcgcg gtaattcccc acgtcgtaga tcgcgagcac gttggcgtgg
     3001 ttgatccgcg ccatcacctg ggcttcgacc agcaggtgcg tgcgcatgcc acccacgccc
     3061 cgagcaccgc gacgcaggag cttgagcgcg accttgcgat cgagctcggg gtcataagcc
     3121 agatacacga cgcccatgcc gccgcggccg agcagatcga gcaccacgaa ccggcccacc
     3181 accgcgccct tcgcgaggct ctcgcagtcg ccggtgggat acgctggatg cgtggtgtgc
     3241 gcctcggcca ccgccgacag cagctgcaat ccggggctca tctcgatgac gaggcggcgg
     3301 caggtcgcgc attggtcgac gtgggcctcg agctcggcac gctgctcgat cgacagacgg
     3361 cgctccagga agcccgcgac cgtggactcc gaggggcagg acgcactact ccggacactc
     3421 ccggtcatcg agtgcagtct acggcagcag attgcgggcg cgcgacaggc ttggtaggtt
     3481 cggcgtggca ggccatggca gtggagatca atcgctgctt cgaacactgc cgccaatcgg
     3541 gcgcggtcta cggccgcaga ttgcggcatg cagcaggcag accatgaccg cggagatcga
     3601 ccaggaggcg ctgtgcgcgc tctattcact cggccgcgcg gtgtggccgg agatcgatct
     3661 cgacatcgcg accttctcga cactggcggg gccccggatg agcacggttc gactcgatac
     3721 cctccgcgcc ggcgatctct acctcgcggc ggcgtgcgcg gcgggcgtcg agcgggcgat
     3781 cgcggcgctc gagcagcact atctgtccgg gatcgcgtcc gcgctggtcc gacgcgggta
     3841 cgacgccgcg gcggtcgccg atgccgtgca ggcggtgcgc gtccgcttcc tcgtcggtga
     3901 cgccggccgt gcgccccgga tctcggagta tgacggccgc gggtcgctgg cgacctggat
     3961 ccaggtggcc gcccaccgga tcgcgatcag cgcccaccgc aggcatcatc gggagacggc
     4021 cgacgaggcc gacgtcgtcg cagcggagcg cagcccggag cttgctctgc tcggccagcg
     4081 gttcggcgcc gagttcgaga tcgcgttccg cagcgcgttc gaggcgctca ccgcgcgcga
     4141 gcgcaccttg ctgcgctatc aggtgatcga ccggatcggg atcgaccaga tcgcggcaat
     4201 ccatggcatc caccgcgcga ccgcggctcg ctggatcgcg catgcccgcg aggcgctcgt
     4261 cgagggcgtg cggcgtgcgc tgcaggaccg actcggggtg gatcacgagg aactcgtgag
     4321 cctgctccgc gtggtccaca gccagctcga gctcagcctg cggctgctgt ggaccccgag
     4381 tccggaccac ccggagcgaa acgcgggctg agctgaccac cggcgcggcc gtcgcacgat
     4441 gctcgggctg cggcggtggt ggctacccac cccgtccggc aggatcgtca cgggtcacgc
     4501 ttgccctcgg tggtcgacgt cccgactccc gccgtgttcc cgcccggatc gcaaggtagc
     4561 gatcacgggg ccgaccgggg tggatgtcag cggaggctca gcagttcttg atgaaccaga
     4621 ccggattcca gttgatgcct tgctggccac cgccggggaa gatcgtgccg ggcggattga
     4681 gcgggttatg gttctggtcg tagacgatcg tccctggatt gccggtctgg tcgtcgaagt
     4741 agaatccagt cccgttccag aagttcaccg agtacgtggt gcaagtgaat agcgcgaaga
     4801 ctctccaccc gcccgtgacg gggttcgcga cgacggcgta gaagttgccc ggcgagacaa
     4861 tgtacgtgcc gttgctcgcg atgtcttgga aggacgtcgc gggtgagatg ctcggggcgg
     4921 tggcaaactg cgatccagcc cccttgccct ggtcgagcgc cgtcggcgca gtgccgtccg
     4981 gggccggctg cgggatggag ccctccggtc ctccctgcgg ctccatgccg ccatctcctg
     5041 gtgccacgca tgcgacgagc agactggcca gcgcgctcac gagcaccaca ctgcaactcg
     5101 tccggttctt accactcttc atgatcatcc tccgtgctgt tgttgaaggc tgccccgaaa
     5161 caggcaatga tagacgtcga atccatcccg tttcgcatcg gatcctcgca tcggccgatc
     5221 gaccggcgag ggaggcgccg actgcggccg cgggccatcg tcttcatcgg cttgtcagat
     5281 gacgcacaga agcaacgtga gtcgagtcga tgtccccccc ggcagcgcgg cgcgccggtt
     5341 gatccgttcc tcgaagagac ctcgtccggg tccgccccac ccgccggcga gctcgatgcc
     5401 cgggtacgcg ggccagcgaa ccatgtcgca cgctcctgcg cagatcttcc gtgattgcga
     5461 tctaacgata gacgccgggc gccagccggt gatcgccgcg catggccacc gtcactgcag
     5521 gtagctggcg atggcgtgca cgatctcggg cgtgacgtgc gccgcggcgc ccttgccggc
     5581 acgctggagc tggacgccga gcgtgatcgg cacggtgccg cgcgcggtga ccacgccgaa
     5641 cgcgatcacg aacaggctgt cgtcgggcgg ggtcttgccg cacgtgagct gggcggccgg
     5701 cgggtggcga tcgttccacg cgcggcacgc cgcgtccttg tgcgcgatgt cggcgagcga
     5761 gtcggtggtc ccggtcttgg cgccgtagat gaggagcttg ggctcggcga gcagcgcgcc
     5821 gatctgggcg tgggcggtgc cggcgacctg gaccaccgcg cacaggccgc cgtcggcgcc
     5881 gagcacggtg ttggcgtggc cgtcggtctt ggcgcacgac cacgccgggg tgacgccgac
     5941 cgcgggcgcc gggaatcacg acgcggcgag ccgcggcgag c
//
LOCUS       DASVOR010000034         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_380270,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000034 DASVOR010000000
VERSION     DASVOR010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380270"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VF516_00765"
     CDS             <1..520
                     /locus_tag="VF516_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106219.1"
                     /translation="HRCVLAVPAGSRLREQALGELAQLADVGLDPVLLGASKLADRYL
                     TRAPTRPAADKLTISVVPTPVPAGKSFPQIPDKLSSAELRPALVACWEAYSAATHKDA
                     LTVTIGLRSVYTPSEYEDEPGTVAVKLDPPAPGGGPDAAADACVRQVVEPAIKSLRLT
                     DAFTTRLALTVR"
     gene            570..1682
                     /gene="rlmN"
                     /locus_tag="VF516_00770"
     CDS             570..1682
                     /gene="rlmN"
                     /locus_tag="VF516_00770"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEX8106220.1"
                     /translation="MDKLDLRTVTLDEAEAFAVAQGWPRFRGEQIWRWVHDQGARSFD
                     DMTNLGRDTRARLAETAALGGLEIAEVQTSHDGTRKLRLVTRDGQSIESVSIPDGDKT
                     TQCISSQVGCAVDCQFCATAKLGLRRNLDAGEIVDQVYLARRLLAEVEPGRRISNLVY
                     MGMGEPLHNYDNVVRSLRILTHDKGVGLSQRRITVSTSGLVPKLERLGGEDVRPNLAV
                     SLNAPSDAIRDAIMPINRKWNIAKLLGALRGYPLEQRRRITFEYVLLAGVNDSLEDAA
                     QLARLLRGIRCKVNLIPFNPHPEAPYARPSHAAVAAFQDECRRLGLPVYLRTPRGDDI
                     DAACGQLANRSGGAPLVPLRVRPRRSDELPPAPAVE"
     gene            2122..>2883
                     /locus_tag="VF516_00775"
     CDS             2122..>2883
                     /locus_tag="VF516_00775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106221.1"
                     /translation="MAAPGPPPTPRPPGRALLRLAGVLTQLTCGACLLIGTIGLVLVE
                     QNRTGSLAPVVAWLVTAMAGLVFGGLIYRGGLVSMLVAASIDAGFGIVLVTFEHDSLR
                     RLLKILRPSDVAAIHDALGIAGFVMIGAGVICLVALPQGIRYARWFRDAAASRSAMST
                     SEARRWGVGAPPALPEGRGARSPGGSTSRGFPPPPVPVRSSAFIIPAEDQPGARRRLY
                     LVLGGGAIGVGVAIGALVSSIRAGAAEPEAVVATGA"
BASE COUNT          362 a         1059 c         1068 g          394 t
ORIGIN      
        1 gcatcgctgc gtgctcgcgg tcccggcggg cagccggctg cgcgagcagg cgctcggcga
       61 gctcgcgcag ctcgccgacg tcggcctcga tccggtgctg ctcggggcca gcaagctcgc
      121 cgaccgctac ctgacccgcg ccccgacgcg gccggccgcc gacaagctca cgatctcggt
      181 cgtgccgacg ccggtgccgg ccggcaagag ctttccgcag atcccggaca agctgagctc
      241 ggccgagctg cggccggcgc tggtcgcgtg ctgggaggcg tacagcgcgg cgacccacaa
      301 ggacgcgctg accgtgacga tcgggctcag gtcggtgtac acgccgtcgg agtacgagga
      361 cgagccgggc acggtcgcgg tcaagctcga cccgccggcg ccggggggcg gccccgacgc
      421 ggccgcggac gcctgcgtcc gccaggtcgt cgagccggcg atcaagagcc tgaggctgac
      481 cgacgcgttc accacccggc tggcgctcac cgtcaggtga ccgcccgacg gcaactacgc
      541 gaacgccgcg agctgtggta gccaggggcg tggacaagct cgatctgcgg acggtgacgc
      601 tcgacgaggc cgaggccttc gccgtggcgc agggctggcc gcggttccgc ggcgagcaga
      661 tctggcgctg ggtccacgac cagggcgcgc gcagcttcga cgacatgacc aacctcggcc
      721 gcgacacccg ggcgcggctc gccgagaccg ccgcgctggg cgggctcgag atcgccgagg
      781 tccagacctc gcacgacggc acccgcaagc tccggctggt cacccgcgac gggcagtcga
      841 tcgagagcgt gagcatcccc gacggcgaca agaccacgca gtgcatctcg tcgcaggtcg
      901 gctgcgcggt cgactgccag ttctgcgcga ccgccaagct cggcctgcgc cgcaacctcg
      961 acgcgggcga gatcgtcgat caggtgtacc tcgcgcgccg gctgctcgcc gaggtcgagc
     1021 ccggccgccg gatctccaac ctggtctaca tgggcatggg cgagccgctc cacaactacg
     1081 acaacgtcgt gcgctcgctc cggatcctga cccacgacaa gggcgtcggg ctgtcgcagc
     1141 ggcggatcac ggtgtcgacc tccggcctgg tgcccaagct ggagcggctc ggcggcgagg
     1201 acgtgcggcc caacctcgcg gtgtcgctca acgcgcccag cgacgcgatc cgcgacgcga
     1261 tcatgccgat caaccgcaag tggaacatcg cgaagctgct cggcgcgctg cgcggctatc
     1321 cgctcgagca gcgccggcgg atcacgttcg agtacgtcct gctcgccggc gtcaacgact
     1381 cgctcgagga cgccgcccag ctggcgcggc tgctgcgcgg catccgctgc aaggtcaacc
     1441 tgatcccgtt caatccgcac cccgaggcgc cgtacgcgcg cccgtcgcac gccgcggtcg
     1501 ccgcgttcca ggacgagtgc cggcggctcg gcctgccggt gtatctgcgc acgccgcgcg
     1561 gcgacgacat cgacgccgcg tgcggccagc tcgcgaatcg atcgggcggt gcaccgctcg
     1621 tgccgctgcg ggtccggccg cggcgctccg acgagctgcc gccggcgccc gcggtcgagt
     1681 gagccttccc tccggggcat gaccgtctgc gaccgcgatg tcgctctgac atggcggatg
     1741 tggccatggc cgtggcccgc gcctggagat cagcgtggtt cgcgggtgtg tggcggcgca
     1801 cgtccccgtg ggataacccg ccgaacgtga cggatgcgcg ccgcgtaccg gccgccgggc
     1861 gaccgaaacc tgccctggcc cgtatgaata ggcgtgggcg cctcgcgcgt tgcatcgtcc
     1921 gtgggcagcg tcggaacccg gccgcgtcgg atcaccgcct cgtgggtagg cgcccccgat
     1981 cctgccgcct cgccgcgccc cggatcctca ccgcgcggtg gcttcccgcc gaccggtccg
     2041 tggctgcagg cggggtcgga ccccgggctc gccgaccaga atctggcggt ccggcgggag
     2101 tcccggagta cgatcgcggc ggtggcagcc ccgggtcctc caccgacgcc tcgtccgcca
     2161 gggcgggcgc tgctgcgcct cgcgggcgtg ctgacccagc tgacctgtgg cgcgtgcctg
     2221 ctgatcggca cgatcggcct ggtgctggtc gagcagaacc ggaccggcag cctcgcgccg
     2281 gtcgtcgcgt ggctggtcac cgcgatggcg ggcctggtgt tcggcggcct gatctatcgc
     2341 ggcggcctgg tcagcatgct ggtcgcggcc tcgatcgacg ccgggttcgg catcgtgctg
     2401 gtcacgttcg agcacgacag cctgcgccgg ctgctcaaga tcctgcgccc gtcggacgtc
     2461 gccgcgatcc acgacgcgct cggcatcgcc gggttcgtga tgatcggcgc cggcgtgatc
     2521 tgcctggtcg cgctgccgca gggcatccgc tacgcgcggt ggtttcgcga cgcggcggcg
     2581 tcccgcagcg cgatgtcgac gagcgaagcg aggagatggg gggtgggggc cccgccggct
     2641 ttgccggagg ggcggggggc aaggtccccc gggggatcga cctcgcgcgg gttcccgcca
     2701 ccgccggtcc cggtccgcag ctccgcgttc atcatccccg ccgaggacca gccgggcgct
     2761 cgccggcggc tgtacctggt gctcggcggc ggcgcgatcg gcgtcggcgt ggccatcggc
     2821 gcgctggtgt cgtcgatccg ggccggcgcg gcggagcccg aggctgtggt ggccaccggc
     2881 gcg
//
LOCUS       DASVOR010000035         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_117321,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000035 DASVOR010000000
VERSION     DASVOR010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117321"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..760)
                     /locus_tag="VF516_00780"
     CDS             complement(<1..760)
                     /locus_tag="VF516_00780"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX8106222.1"
                     /translation="MQRYLAAAGVAARRKAEELITAGRVTVNGAVVTVLGTRIDPEHD
                     AVEVDGEAVVALDQFYVLFNKPKGCITAGTDDRGRPTVMDYLPNLPVPVKPVGRLDFY
                     SEGVLLLTNDGELAARLLAPASHVPKTYHVKVHGRLERADLTALRSGITLDDGTTTLP
                     AEIEILPGESKHTWLGITIHEGKHRQIHRMIEALGHRVDKLQRVAFANLTFHDLRVGD
                     ARELTQQELNELRDSVGLDHGAVARGRWRARREDT"
     gene            complement(781..>2077)
                     /gene="scpB"
                     /locus_tag="VF516_00785"
     CDS             complement(781..>2077)
                     /gene="scpB"
                     /locus_tag="VF516_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEX8106223.1"
                     /translation="EATEGTDHVADDEGRDAAGDDDDAAGVFPTSAATMDAVQLKHLV
                     EALIFAADRPLTVQRLRQLTRVSDVRRLEQALAEINEDYRERGLVLQQVSGGYQFRTR
                     TQFSPWVQQLIAGRPVRLSRAQLETLAIIAYRQPITRPEIDDIRGVDSSATLKLLIDR
                     ALIRVLGKREEVGRPMLYGTTKEFLDFFSLGDLRELPTLREYSELTAESRKVMSDRLG
                     IAVDGSDGPDGGGEDPDGGSGNGHGGNGHGSNGHGGYGGDDGGAGGPSGLGGGGLSED
                     GPAASAAADVADRGGTAAEDAADASSLAEDVGAVSAAEDAAGASSSAGAVNAAEDAAA
                     ATSDVAKDRELADASGADAPDGAAPDAVSGALSAFWAASDAVDRSNAAALGDSEDDAP
                     ADTWFDPGIGDSLDDLVDTDDPDEPGDARRPNTGPRAAE"
BASE COUNT          276 a          779 c          704 g          318 t
ORIGIN      
        1 cggtgtcctc gcgccgagcg cgccatcgac cgcgagcgac cgcgccatga tcgaggccga
       61 ccgagtcgcg gagctcgttg agctcctgct gggtcagctc gcgggcgtcg ccgacccgga
      121 ggtcgtgaaa cgtcaggttg gcgaacgcga cccgctgcag cttgtcgacc cggtggccga
      181 gcgcctcgat catccggtgg atctggcggt gcttgccctc gtggatcgtg atgccgagcc
      241 aggtgtgctt gctctccccg gggaggatct cgatctcggc gggcagcgtg gtcgtgccgt
      301 cgtcgagcgt gatgccgctg cgcagcgcgg tgagatcggc gcgctcgagc cggccgtgga
      361 ccttgacgtg gtaggtcttg gggacgtggc tggccggggc gagcagccgc gcggccagct
      421 cgccgtcgtt ggtcaggagc agcacgccct cggagtagaa gtcgaggcgg ccgaccggct
      481 tgaccggcac cggcaggttc ggcaggtagt ccatcaccgt cggccggccg cgatcgtcgg
      541 tccccgcggt gatgcagccc ttcggcttgt tgaacagcac atagaactgg tcgagggcaa
      601 ccaccgcctc gccgtcgacc tccaccgcgt cgtgctccgg gtcgatccgg gtgccgagca
      661 cggtgacgac cgccccgttc accgtcaccc ggcccgcggt gatcagctcc tctgccttgc
      721 ggcgtgcggc cacccccgcc gccgcgaggt accgctgcaa acggaccggc tcggccatcg
      781 ctactccgcg gcccgaggac cggtgttcgg acgcctcgcg tcaccgggct cgtcgggatc
      841 gtccgtatcg accagatcgt cgagcgagtc gccgataccc ggatcgaacc aggtgtcggc
      901 aggcgcatcg tcctcgctgt cacccagcgc ggcggcgttg gagcgatcga cggcatccga
      961 ggccgcccag aacgcagaca gcgccccgga gaccgcgtcg ggcgccgcac cgtccggcgc
     1021 gtccgctccc gaggcatccg caagctcgcg gtccttcgcc acgtcgctcg tcgcggcggc
     1081 agcgtcctcc gccgcattca ccgctccggc cgagctgctc gcaccagcag catcctccgc
     1141 ggcactcacc gcgccaacgt cctcggccaa gctgctcgca tcggcggcgt cctccgcggc
     1201 ggtccccccc cgatccgcaa catccgcagc ggcgctcgca gccgggccat cctccgacag
     1261 cccgccgcct ccgaggccgc tcggaccgcc ggcgccccca tcgtcaccgc cgtagccgcc
     1321 gtgcccgttg ctgccgtggc cattcccgcc atgaccattg ccggagccgc catcgggatc
     1381 ctcgccgcct ccatcagggc catcgctgcc gtccaccgcg atgccgagcc gatcgctcat
     1441 gaccttgcgg ctctcggcgg tgagctcgga gtactcgcgc agggtcggca gctcgcgcag
     1501 atccccgagg ctgaagaagt cgaggaattc cttggtcgtg ccgtacagca tgggccggcc
     1561 gacctcctcg cgcttgccga gcacgcggat cagcgcgcgg tcgatcagga gcttgagcgt
     1621 ggcgctcgag tcgaccccgc ggatgtcgtc gatctccgga cgcgtgatcg gctggcgata
     1681 ggcgatgatc gcgagggtct ccagctgcgc ccgcgacagc cggaccggcc ggccggcgat
     1741 cagctgctga acccacggtg agaactgggt ccgggtccgg aactggtagc cgccggacac
     1801 ctgctgcagc accagcccgc gctcgcggta gtcctcgttg atctcggcca gcgcctgctc
     1861 caggcggcgc acatcgctga cccgggtcag ctggcgaagc cgctgcacgg tcagcggtcg
     1921 atccgccgcg aagatcagcg cctcgaccag gtgcttgagc tggacagcgt ccatcgtcgc
     1981 ggcgctggtc gggaacacgc ccgcggcatc gtcgtcgtcg cccgcggcat cacgaccctc
     2041 gtcgtccgcg acgtggtccg taccctcggt cgcctcg
//
LOCUS       DASVOR010000036         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_555694,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000036 DASVOR010000000
VERSION     DASVOR010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555694"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1145)
                     /locus_tag="VF516_00790"
     CDS             complement(<1..1145)
                     /locus_tag="VF516_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8106224.1"
                     /translation="MAFQDLFRGPASVLALSASYVVGRSAAAPSAVNERGLRKLSASL
                     ERAAIGAAAAIRIGRQVRNAPVLRSRSRLLARPDELVPVCTLISDTHVIAPGRSLCEL
                     DADPGQWPWREMPTCEVIAGGLGRVLEHASRHAPHTVIWCGDEVDTGDPAEWQQWRQV
                     VDGVPRLMHRLVPGNHDICFNRPHDADYTLARRAARETAYQQHAGRLGDFPVVDTLIT
                     GAGAVTVVLLDSCRHRSTHILSNAVGRFDDDQLDELERILDPVAGPLLVISHHHVWRD
                     APFLQPDAWYNTVVDADRLAAILGDYRQRAPGNQVLVCHGHRHALSAGVIGEPGAEIS
                     VIGLPSTTLGDKSTTGMLDGVLRYAIAGLRRDGSWAVALRPVGELTRP"
     gene            1400..>1614
                     /locus_tag="VF516_00795"
     CDS             1400..>1614
                     /locus_tag="VF516_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106225.1"
                     /translation="MTVSRNRRPWIATGLALAASSASCAVQAPAEPASDGVDDAAAVA
                     RRAHPGPRTPIQHAISVVGENHSCDNL"
BASE COUNT          242 a          597 c          585 g          190 t
ORIGIN      
        1 ggccgggtca gctcaccgac cgggcgcagc gcgaccgccc agctgccgtc gcggcgcagc
       61 ccggcgatcg cgtagcgcag caccccgtcg agcatgccgg tcgtcgactt gtcgccgagc
      121 gtggtcgacg gcaggccgat cacgctgatc tcggcgccgg gctcgccgat cacgccggcg
      181 ctcagcgcgt ggcggtggcc gtgacagacc agcacctggt tgcccggcgc gcgctggcgg
      241 tagtcgccga ggatcgcggc cagccggtcg gcgtcgacca cggtgttgta ccaggcgtcg
      301 ggctggagga acggggcatc gcgccacacg tggtggtgcg agatgaccag cagcgggccc
      361 gcgaccggat cgaggatgcg ctcgagctcg tcgagctggt cgtcgtcgaa ccggccgacg
      421 gcgttggaca ggatgtgggt cgagcggtgg cggcacgagt cgagcagcac cacggtgacc
      481 gcgccggcgc cggtgatcag ggtgtcgacc accgggaagt cgcccagccg gccggcgtgc
      541 tgctgatagg cagtctcgcg cgccgcgcgc cgcgccagcg tgtagtcggc gtcgtgcggg
      601 cgattgaagc agatgtcgtg attgcccggc accaggcgat gcatcaaccg cggcacgccg
      661 tcgaccacct gccgccactg ctgccactcc gccggatcgc cggtgtcgac ctcgtcgccg
      721 caccagatca cggtgtgcgg cgcgtgccgg ctggcgtgct cgagcacgcg gcccaggcca
      781 ccggcgatca cctcgcaggt cggcatctcg cgccatggcc actggcccgg atcggcgtcg
      841 agctcgcaca gcgagcggcc cggagcgatc acgtgggtgt cggagatcag ggtgcagacc
      901 gggacgagct cgtcgggccg cgccagcagg cgggagcgcg agcgcagcac cggcgcgttg
      961 cggacctgcc ggccgatccg gatcgccgcg gcggcgccga tcgcagcgcg ctccaggctg
     1021 gcgctcagct tgcgcagccc gcgctcgttc accgccgacg gcgccgcggc ggatcgcccc
     1081 acgacgtacg acgccgacag cgccagcacc gacgccgggc cgcggaacag atcctggaac
     1141 gccacgcgct cagccgacca cgccccggta acgcgaccgc gacaccgcgc cgacggtcgg
     1201 gtgccatcac ggcgaactgc agcgcgccat gccagcgccg cgcttcgtac cgtgcggcac
     1261 cgctcgcccg acgggagatc ggcatccgtc acgccgccat cgagccgaca caggaccagg
     1321 ttcggcgcga cacgcgcacc cgcgagattg gcgccgccgt gccagcggcc ggcaccttcc
     1381 acttcgcaaa ggatggacca tgacagtatc gaggaacagg cggccatgga tcgccacggg
     1441 actcgcgctc gcagcgtcga gcgcgtcgtg cgccgtacag gcccccgccg aaccggcgag
     1501 cgacggcgtg gacgacgccg ccgcggttgc acgccgcgcg caccccggac cgcgaactcc
     1561 gatccagcac gcgatcagcg tggtcggcga gaaccactcc tgcgacaacc tgtt
//
LOCUS       DASVOR010000037         3695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_175748,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000037 DASVOR010000000
VERSION     DASVOR010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175748"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..1249
                     /locus_tag="VF516_00800"
     CDS             86..1249
                     /locus_tag="VF516_00800"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEX8106226.1"
                     /translation="MSLEDDALDELRELEALHRRRMPRVRDGVPGAVIRLDGRDVLNY
                     ASNDYLGLAGDPRLIRAAAAALDDGGTGASASRLIIGNHRHHVRLESAVADWLRCGGV
                     RLFNSGYAANVGVLTALLRAGDVVFSDELNHASIIDGCRLSRAEVTVFRHRDVAALEA
                     ALLDRVGCRRIVVSESLFSMDGDLADVEAIAALCKRHDAAFLLDEAHAIGVIGPEGRG
                     LAALHGVVPDIVIGTFGKALGGFGAFAATSRAVAELLWNKARPLVFSTGLPASVPAAA
                     QTAIEIVRGVDGEHRRARLARHARRLRALVPGIAGASASAIAPLVIGEDRAAMRCTDR
                     LLAEGIFVQGIRPPTVPPGTARLRVGLSAAHSEQHIETAVESLNRLLAAEGLR"
     gene            1246..2520
                     /gene="bioA"
                     /locus_tag="VF516_00805"
     CDS             1246..2520
                     /gene="bioA"
                     /locus_tag="VF516_00805"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEX8106227.1"
                     /translation="MSDVADLDRQVMWHPFTQMAEWEPLVIASGDGNYLIDRGGRRYL
                     DGVSSLWCNVHGHRHPRLDRALRDQLDRIAHSTFLGLSHEPAVRLGAALLEVAPPGLR
                     RVFYSDSGATAVEIALKQSFQYWQLRGRARKQRFLRLDEAYHGDTLGAVGVGGIELFH
                     RIFGPLLVRSTAIPSPAGSDGSAALAALEQQLAAHADELAAFVLEPLVQGAAGMLVHP
                     RGFLARAADLCRRHGVHLIVDEVATGFGRTGTLFACEQESIAPDFLCLAKGISGGYLP
                     LAATLTTEEIYAAFLGSQHELKQFFHGHTYTANPLACAVGLESLALLRESTLANARAQ
                     IPRFAAALDRIAGIAGVRSTRRCGLMAGIELHPRPPRHLGNEVCARVRSHGVILRPLG
                     DVVVWMPPLTLQPADLDLLERATTAAIREVLA"
     gene            2517..3200
                     /gene="bioD"
                     /locus_tag="VF516_00810"
     CDS             2517..3200
                     /gene="bioD"
                     /locus_tag="VF516_00810"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HEX8106228.1"
                     /translation="MNGVFVTGTDTNVGKTYVACALARRARDRGHKVFAFKPIETGCT
                     PTDRGYLGADQELLAAAAGDWQTGPLRGLYRFPLPAAPLVAAQQAGSAIDLDRVERTA
                     RDGAVQAGATWLLVEGAGGWRVPITPDADMAVLARRLQLPVLVVARAGLGTINHSLLT
                     LEAVERDGLPVAALVLSRRETDDPAVAESNLAQIQRRWAGRILILSADLLVLDRLLPA
                     ASQTSGSPS"
BASE COUNT          471 a         1436 c         1234 g          554 t
ORIGIN      
        1 catcggtaag tgacctcgcg ggtgcgatcg cggaatacgc aggaagcgct ttgcaggcgc
       61 gccgcggtgg ctatagtcgc ccgcggtgag cctcgaagac gatgcgctcg acgagctccg
      121 cgagctggag gcgctgcacc ggcggcgcat gccgcgcgtg cgcgacggcg tgcccggcgc
      181 cgtgatccgg ctcgacggcc gcgacgtcct caactacgcc tccaacgact acctcggcct
      241 cgccggggac ccgcggctga tccgggccgc cgcggcggcg ctcgacgacg gcgggaccgg
      301 cgcgagcgcg tcgcggctga tcatcggcaa ccaccgccac cacgtgcggc tcgagtccgc
      361 ggtcgcggac tggctgcgct gcggcggggt ccggctgttc aactccggct acgctgccaa
      421 cgtcggcgtg ctgaccgcgc tgctccgcgc cggcgacgtg gtgttctccg acgagctcaa
      481 ccacgccagc atcatcgacg gctgccggct gtcgcgcgcc gaggtcacgg tgttccgcca
      541 ccgcgacgtc gctgcgctgg aggccgcgct gctcgaccgc gtgggctgcc gccggatcgt
      601 ggtgtccgag tcgctgttct cgatggacgg cgatctcgcc gacgtcgagg cgatcgccgc
      661 gctgtgcaag cgccacgacg ctgcgttcct cctcgacgag gcccacgcga tcggcgtgat
      721 cggaccggag ggccgcggcc tcgcggcgct gcatggcgtg gttcccgaca tcgtgatcgg
      781 cacgttcggc aaggcgctcg gcggcttcgg cgcgttcgcc gcgacctcgc gcgccgtcgc
      841 cgagctcctg tggaacaagg cccgcccgct ggtgttctcg accgggttgc cggcgagcgt
      901 cccggcggcc gcgcagaccg cgatcgagat cgtgcgtggc gtcgacggcg agcaccggcg
      961 cgcccggctg gcccggcacg ctcgccggct gcgcgcgctc gtcccgggga tcgcgggcgc
     1021 ctcggcgagc gcgatcgccc cgctcgtcat cggcgaggat cgcgccgcga tgcgttgtac
     1081 cgaccgcttg ctcgccgagg ggatcttcgt ccagggcatc cgtcccccga cggtgccgcc
     1141 gggaaccgcc cgcctccggg tcggcctcag cgcggcccat tcagagcaac acattgaaac
     1201 tgcagttgaa tctctgaacc ggctgctcgc cgctgagggc ctgcgatgag cgacgtcgcc
     1261 gacctcgacc ggcaggtcat gtggcacccg ttcacgcaga tggcggagtg ggagccgctc
     1321 gtgatcgcct ccggtgacgg caactacctc atcgaccgcg ggggccggcg ctacctcgac
     1381 ggcgtcagct cgctgtggtg caacgtccac ggacatcgcc atccgcgcct cgatcgcgcc
     1441 ctgcgcgacc agctggaccg gatcgcgcac tcgacgttcc tcggcctgtc ccacgagccg
     1501 gcggtccgcc tgggcgccgc gttgctcgag gtcgcgccgc caggcttgcg ccgcgtcttc
     1561 tactccgact ccggggcgac cgccgtcgag atcgcgctca agcagtcgtt ccagtactgg
     1621 cagctgcgcg ggcgcgcccg caagcagcgg ttcctccgcc tcgacgaggc ctaccacggc
     1681 gacaccctcg gggcggtcgg cgtcggcggc atcgagctgt tccaccggat cttcgggccg
     1741 ctgctggtcc gcagcaccgc cattccgtcg ccggccggca gcgacggcag cgccgcgctc
     1801 gccgcgctcg agcagcagct cgccgcgcac gccgacgagc tcgccgcgtt cgtgctggag
     1861 ccgctcgtcc agggcgcggc cggcatgctc gtccatcccc gcggcttcct cgcacgggcg
     1921 gccgacctgt gtcgccgcca cggcgtgcac ctgatcgtcg acgaggtcgc cacgggcttc
     1981 ggccgcaccg gcaccctgtt cgcctgcgag caggagagca ttgcacccga cttcctctgc
     2041 ctcgccaagg gtatctccgg cggctacctg ccgctggccg ccactctcac caccgaggag
     2101 atctacgccg ccttcctcgg gtcccagcac gagctcaagc agttctttca tggccacaca
     2161 tacacggcca acccactggc gtgcgccgtc ggcctggagt cgctggcgct gctgcgcgag
     2221 tcgaccctcg ccaacgcgcg cgcccagatc ccccgcttcg ccgcggcgct cgaccggatc
     2281 gccggcatcg ccggcgtgcg ctcgacccgc cggtgcggcc tgatggcggg catcgagctc
     2341 catccgcggc cgccgcgcca cctgggcaac gaggtctgtg cgcgcgttcg cagccacggc
     2401 gtgatcctcc ggccgctcgg cgatgtcgtg gtctggatgc cgccgctgac gctccagccc
     2461 gcggacctcg acctgctgga gcgagccacc accgccgcga tccgcgaggt gctcgcgtga
     2521 acggcgtctt cgtcaccggc accgacacca acgtcggcaa gacctacgtc gcctgcgcgc
     2581 ttgcccgtcg agcgcgtgac cgcgggcaca aggtcttcgc gttcaagccg atcgagaccg
     2641 gctgcacgcc gaccgaccgt gggtacctcg gcgccgatca ggagctgctc gccgccgcgg
     2701 cgggcgactg gcaaaccggc ccgctacgcg gcctctaccg ctttccgctt cccgccgcac
     2761 ctctcgtcgc cgcccagcag gcggggtccg cgatcgatct cgatcgggtc gagcggaccg
     2821 cgcgcgatgg cgcggtgcag gccggggcga cctggctcct cgtcgagggc gccggtggct
     2881 ggcgcgtccc gatcaccccg gacgccgaca tggctgtcct cgcgcgccgc cttcagcttc
     2941 ccgtcctcgt cgtggcccgc gccggcctgg gcaccatcaa ccacagcttg ttgaccctgg
     3001 aagctgtcga gcgcgacggc ctgccagtgg ccgctctcgt cctctcgcgc cgcgagaccg
     3061 acgacccggc ggttgccgag agcaacctgg cccagatcca gcggcgctgg gcgggcagga
     3121 tcttgatcct gtccgccgat ctgctcgtgc tcgatcgtct gctgcctgcc gcaagtcaga
     3181 cctccgggtc gccgtcgtga cggcccgcgc gggcggctca tcgaccaccg ctgggttgtc
     3241 gcgccgcgac atccgcaacc tcgtttcggg tcgggacagc ctcgtctagc gctttcccgg
     3301 ggcgttccac gtggaacacc gccccaatga tgttccacgt gaaacatacc ggaccgcgtt
     3361 gctccgcgcc gacaatcgcg tcttctctct ggcacctcgg cgaactgagc gtcaccgagc
     3421 ctccccgaca tcccccaacg gggccatcgc cgaagctgga cccgccgctc cacgtgggag
     3481 accggatcgg cgcgagacca agcacagcgc tcgaggcccc tcatcgcctt ctcgcgcccc
     3541 atgcccggcc cggacccgca ccggcctcac cgcgcaacga tgttccacgt gaaactacca
     3601 gacggcattg tcccacgccg acgctcgcgc ctcccccccg atgcgatcgg cgaagctgga
     3661 ccgccctccc cacgcggggg atcgggatcg acctg
//
LOCUS       DASVOR010000038         6099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_473,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000038 DASVOR010000000
VERSION     DASVOR010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6099
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VF516_00815"
     CDS             complement(<1..594)
                     /locus_tag="VF516_00815"
                     /inference="COORDINATES: protein motif:HMM:NF014998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX8106229.1"
                     /translation="MVKRLTPSDAMFLYSESREQMMHIAGLMPFTPAPDSPPDLLRRL
                     MDELRGPLPVCSPWNLKLRTPDLLWNPLQAWVEEPNVDLDYHIRRSALPSPGDERELG
                     ILVSRLHGYRVDFHRPPWEIHFIEGLERGRFAWYAKIHHALIDGYSAMRVMTHALSSD
                     PDERDRPLFFSIPPPERPRPAPATGEGGAAATGEAEHE"
     gene            643..903
                     /locus_tag="VF516_00820"
     CDS             643..903
                     /locus_tag="VF516_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106230.1"
                     /translation="MSACRPGCGACCIAPSISPVLPALPHGKPAGVRCPHLTAEIRCA
                     LFGSPDRPAVCASLRPSPEMCGASPAEAMAYLVALEALTAPR"
     gene            1310..1555
                     /locus_tag="VF516_00825"
     CDS             1310..1555
                     /locus_tag="VF516_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106231.1"
                     /translation="MFEYVPARCAFAHTGGANPRVAYLAGLACGASDRRAVLDGALGP
                     IALTCGRARRWDALPQRFHSDIFAATGAERSRTLGGA"
     gene            1624..5811
                     /locus_tag="VF516_00830"
     CDS             1624..5811
                     /locus_tag="VF516_00830"
                     /inference="COORDINATES: protein motif:HMM:TIGR03903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM system kinase/cyclase fusion protein"
                     /protein_id="HEX8106232.1"
                     /translation="MKDAHDLRAGSVLQGRYEIVSAIGSGGFGAVYKAIQLATHQPVA
                     VKVMHPLADEPEAKRDNRVARFRREMDLCARLQHPNIVGLVDSGQTDDGRLFAAFQFA
                     SGRGLDRVLLEDGPMSPREARYLMSQVLDALSCAHNLGVVHRDLKPANIMLVSTGTRR
                     NALVLDFGIGALAHDLTDPSQAKLTGQHEWLGTPHYTAPEQIRGYPPTSQSDIYSWGL
                     VYLECMLGYPVIAGTPVMAWMFHLGPDPVPIPPQLRHHPLGRLLQRAVIKDVTERTAT
                     ASNLLRELDDCDVSDLERFAGTIPATMESNRPGLAPRSVVPAARGTNPASLPAAHATP
                     GSHGRTTPSSSSAPGRDRLVDGERRQITAVCCSLSPTPGLDLDDLDALIQTQYEICTR
                     VAEQFHGELAGGLGHQVLVEFGYPTASEDDVVRGARAALAIRAAVAERNAAVGGARRM
                     EIRLGIHTGMVAYDPNESESRVSSRMFGMTTMIASQLSAAAAADTIVASAATNQALRA
                     HIVMTPIGTHVIEGVGRGIELYRVESVRARAVVRDTQEGGPARPLVGRDREMALLLER
                     WTQVAAGAGQSVLVTGEAGIGKSRLATELSRRIGARSHTLLEARCTLETCNRVLHPIL
                     EVLERVLDLGDIESEQRLDRIEAALIEFGFRPGDVVPLVASLLSVSHSDRYPAIELAP
                     NRRRELTLDMIVSLLIELSERTPVVLFVEDLHWADPTTLDLLSALVAAIPSSRVLALF
                     TARPDFTPPWPNSAADQIQLSRLTRPQVEQIVAMLTGGKALPGGVLEQMVTRADGVPL
                     FVEELTRMVLESGALTPRGDLYVLTGSLSEVAIPTTLRASLMARLDRLGRAKKTAQLA
                     AAIGREFDLALLTAVGSLDEASVQEDLERLAAVDLVHHKRRLRNPTWLFRHALIRDTA
                     YESMPRRVQQKVHARIAEVIEQQFPEMAHARPDLLALHHAAADQKTHAIRYAQKAALN
                     ALMGSAYSHAIHHAKEAIGWLDAIPARIAAASASSSGVIDLAHASEEAERMDRIEAEL
                     GLRVTLGVPLMLTQGFASREVEANYLRLLELCQLAGDRAVARQFPALWGLWTVRMSSG
                     DHAGAQEAAARLTTLGERTGDSGIRLAALTAHGAAVMMRGRLGEARRAFEDALSLYDP
                     IAHAGLAVLFGQDAGAMCASFLTWVHAHDTDRTHAETRAAEAMEMCDALGQPSTRAFV
                     ETVLATWRCLRGDFAEAERHSDVALRLAADQGMPHWHAQAQITRGWAIAGLGRPAKGA
                     AIARAGINALTGIGSKAGMTFYWGALAEAELAAGRLDQATTALEEAVRYMALSGERIH
                     QPGLALIEARIAVAAGQRDAADDALTRALSVAYQQEAGEVARRAKELRAQLDSTPHSP
                     APRNTPGSIPAVPDILS"
     gene            complement(5828..>6099)
                     /locus_tag="VF516_00835"
     CDS             complement(5828..>6099)
                     /locus_tag="VF516_00835"
                     /inference="COORDINATES: protein motif:HMM:NF024216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8106233.1"
                     /translation="AMVLHATDYLDLFATTVIAWQWLDLAAIAQEALARPLPRGRASR
                     DAGYYRAKLAAAQYWIRTELPRIDHLAALCRDGEDSYLKLDPDWL"
BASE COUNT          811 a         2296 c         2174 g          818 t
ORIGIN      
        1 ctcgtgctcg gcctcgccgg tcgcggccgc cccgccctcg ccggtcgccg gcgccggcct
       61 gggccgctcg ggcggcggga tcgagaagaa cagcgggcga tcgcgctcgt cgggatcgct
      121 cgacagcgcg tgggtcatga cgcgcatcgc gctgtatccg tcgatcagcg cgtggtggat
      181 cttggcgtac caggcgaacc gcccgcgctc gaggccctcg atgaagtgga tctcccaggg
      241 cgggcggtgg aagtcgacgc ggtagccgtg cagccgcgac accaggatgc cgagctcgcg
      301 ctcgtcgccg ggcgacggca gcgccgagcg ccggatgtgg tagtcgagat cgacgttggg
      361 ctcctcgacc cacgcctgca gcgggttcca caagaggtcc ggcgtgcgga gcttgaggtt
      421 ccacggcgag cacaccggca gcgggccgcg cagctcgtcc atcagccggc ggagcaggtc
      481 gggcgggctg tcgggcgccg gggtgaacgg catcaggccg gcgatgtgca tcatctgctc
      541 gcgcgactcg ctgtacagga acatcgcgtc gctcggcgtc aggcgcttga ccacgcggcc
      601 atcctacatg acggcgcggc ggcccacggg gtacgctggc tcatgtccgc gtgccggccc
      661 ggctgcgggg cgtgctgcat cgctccgtcg atctcgccgg tgctgcccgc gctgccgcac
      721 ggcaagccgg ccggcgtgcg ctgcccgcat ctcaccgccg agatccgctg cgcgctgttc
      781 ggctcgccgg accggccggc ggtgtgcgcg agccttcgcc cgtcgcccga gatgtgcggc
      841 gcgtcgccgg ccgaggcgat ggcgtacctc gtcgcgctgg aggcgctgac cgcgccgcgg
      901 tgagcgtcac tccggccggc ggccggcgcg cggcggcgtt gctcgcggca tgctcccgac
      961 gacgttcctc ggtcagccgg cggttctgcg tcgacggcga ggccgtgtgc cagaggatga
     1021 ccagggctgc gagaaagaga agctcgtacc cggcgcgccg agcacggcgt tcctcgtcgc
     1081 cgcgccacgc gaaccgtggc catcgcgctc ccgggtcgcc gccggtgagc cccctgacta
     1141 cacgccggcc accggcgccg cgcgctggac gccgacgagc tcgcgcggct cgcggcgaag
     1201 cgcatggatc tcgcaggatc gcagtcgggg gcccgcggtt caagcgcctg ttctcgctgc
     1261 tgagccgtga tctggggatc gcacgccgtg ttcgcgttcg cgcggccacg tgttcgagta
     1321 cgtgcccgcc cgctgcgcgt tcgcccacac gggcggcgcc aacccgcgtg tggcctacct
     1381 cgcagggctg gcgtgcgggg cgagcgaccg ccgcgcggtg ctcgacggcg cgctcggccc
     1441 gatcgccttg acctgcggcc gcgcccggcg ctgggacgcg ttgccgcagc gcttccactc
     1501 cgacatcttc gcggctaccg gcgctgagcg aagccgcaca cttggcggcg cttgacaacg
     1561 atcgcgttcg tcctttccaa tgaaagtctg cgcgggtgca cggctggggc cccgatggac
     1621 aggatgaaag acgcccacga tctacgcgca gggtcggtcc tgcaaggtcg ctacgagatc
     1681 gtgtccgcca taggttctgg cgggttcggg gcggtctata aggccattca gctggcgacc
     1741 caccagcccg tcgcggtgaa ggtcatgcac ccgctcgcgg acgaacccga ggccaagcgc
     1801 gacaaccgcg tcgcgcggtt ccgccgcgag atggacctgt gcgcgcggct acaacacccc
     1861 aacatcgtcg gcctggtcga ctccggtcag accgacgacg gccggctgtt cgccgcgttc
     1921 cagttcgcgt cgggccgcgg cctggaccgg gtgctgctcg aggacggtcc gatgagcccg
     1981 cgcgaggcgc gctacctgat gtcgcaggtg ctcgacgcgc tgtcgtgcgc ccacaacctc
     2041 ggcgtcgtcc accgcgacct gaagccggcc aatatcatgc tggtgtcgac cgggacgcgg
     2101 cgcaacgcgc tggtgctcga cttcggcatc ggcgcgctgg cccacgatct caccgatccg
     2161 tcgcaggcca agctgaccgg ccagcacgag tggctcggca cgccgcatta caccgcgccg
     2221 gagcagatcc gtggttatcc gccgacctcg cagtcggaca tctacagctg gggcctggtc
     2281 tacctcgagt gcatgctcgg ctacccggtg atcgccggca cgccggtcat ggcgtggatg
     2341 ttccacctcg ggcccgatcc cgtgccgatc ccgccccagc tgcgccacca cccgctcggc
     2401 cggctgctcc agcgcgcggt gatcaaggac gtcaccgagc ggaccgcgac cgcgtcgaac
     2461 ctgctgcgcg agctcgacga ttgcgatgtt tcggatctgg agcgcttcgc cggcaccatc
     2521 cccgccacga tggagagcaa tcgccccggc ctcgccccgc gctccgtcgt gcccgccgcg
     2581 cgcggcacca accccgccag cctgcccgcc gcgcacgcga ccccgggatc gcacggccgg
     2641 accacgccgt cgagctcgtc ggcgcccggc cgcgaccgcc tggtcgacgg cgagcggcgc
     2701 cagatcaccg cggtgtgctg ctcgctgtcg ccgacccccg ggctcgatct cgacgacctc
     2761 gacgcgctga tccagaccca gtacgagatc tgcacccggg tcgccgagca gttccacggc
     2821 gagctcgccg gtggcctcgg ccaccaggtg ctggtcgagt tcggctaccc gacggcgagc
     2881 gaggacgacg tggtccgcgg cgcgcgcgcc gccctggcga tccgggccgc ggtggccgag
     2941 cgcaacgccg cggtcggcgg cgcccgccgg atggagatcc ggctcggcat ccacaccggc
     3001 atggtcgcct acgatccgaa cgagagcgag agccgggtct cgagccggat gttcggcatg
     3061 accacgatga tcgccagcca gctcagcgcg gcggccgccg ccgacacgat cgtcgccagc
     3121 gcggccacca accaggcgct gcgcgcccac atcgtgatga ccccgatcgg cacccacgtg
     3181 atcgagggcg tgggccgcgg catcgagctg taccgggtcg agtcggtgcg cgcccgcgcg
     3241 gtcgtccgcg acacccagga gggcgggccg gcgcggccgc tggtcggacg cgatcgcgag
     3301 atggcgctgc tgctcgagcg ctggacccag gtcgcggccg gcgccggcca gagcgtgctg
     3361 gtcaccggcg aggccggcat cggcaagtcg cggctcgcca ccgagctcag ccggcggatc
     3421 ggcgcgcgct cccacacgct gctcgaggcg cgctgcacgc tggagacctg caaccgggtg
     3481 ctgcatccga tcctcgaggt gctcgagcgc gtgctcgacc tcggcgacat cgagtccgag
     3541 cagcggctcg accggatcga ggccgcgctg atcgagttcg ggttccggcc gggcgacgtc
     3601 gtgccgctgg tcgcctcgct gctgtcggtg tcgcacagcg accgctaccc ggcgatcgag
     3661 ctcgcgccca accgccgccg cgagctcacg ctcgacatga tcgtctcgct gctgatcgag
     3721 ctcagcgagc gcacgccggt cgtgctgttc gtcgaggacc tgcactgggc cgatccgacc
     3781 acgctcgacc tgctcagcgc gctggtcgcg gcgatcccgt cgagccgcgt gctcgcgctg
     3841 ttcaccgccc ggcccgactt cacgccgccg tggccgaact cggcggccga ccagatccag
     3901 ctgtcgcggc tgacccggcc gcaggtcgag cagatcgtcg cgatgctgac cggcggcaag
     3961 gcgctgcccg gcggcgtgct ggagcagatg gtgacgcgcg ccgacggcgt gccgctgttc
     4021 gtcgaggagc tgacccggat ggtgctggag tccggcgcgc tgaccccgcg cggcgacctc
     4081 tacgtgctga ccggctcgct gtccgaggtc gcgatcccga ccacgctgcg cgcatcgctg
     4141 atggcgcggc tcgaccgcct cggccgggcc aagaagaccg cgcagctcgc cgccgcgatc
     4201 ggccgcgagt tcgacctcgc gctgctcacc gcggtcggct cgctcgacga ggcctcggtc
     4261 caggaggacc tcgagcggct cgcggcggtc gacctcgtcc accacaagcg ccggctgcgc
     4321 aacccgacct ggctgttccg ccacgcgctg atccgcgaca ccgcctacga gtcgatgccg
     4381 cgccgggtcc agcagaaggt ccacgcccgg atcgccgagg tgatcgagca gcagttcccg
     4441 gagatggccc acgcccggcc cgatctgctc gcgctgcacc acgccgccgc cgaccagaag
     4501 acgcacgcga tccgctacgc ccagaaggcg gcgctcaacg cgctgatggg gtcggcgtac
     4561 tcgcacgcga tccaccacgc caaggaggcg atcggctggc tcgacgcgat cccggcgcgc
     4621 atcgcagccg cgtccgcgtc gtcgagcggc gtgatcgacc tggcgcacgc ctccgaggag
     4681 gccgagcgga tggaccggat cgaggccgag ctcgggctgc gggtcacgct cggcgtgccg
     4741 ctgatgctga cccagggctt cgcgtcccgg gaggtcgagg ccaactacct ccggctgctc
     4801 gagctgtgcc agctcgccgg ggaccgcgcg gtggcgcgcc agttccccgc gctgtgggga
     4861 ctgtggacgg tccgcatgag cagcggcgat cacgccggcg cccaggaggc cgcggcgcgg
     4921 ctcaccacgc tcggcgagcg gaccggcgac tccgggatcc ggctcgccgc gctgaccgcc
     4981 cacggcgccg cggtcatgat gcgcggccgg ctcggcgagg cccgccgcgc gttcgaggac
     5041 gcgctgtcgc tgtacgatcc gatcgcccac gccggcctcg ccgtgctgtt cggccaggac
     5101 gccggcgcga tgtgcgcctc gttcctgacc tgggtccacg cccacgacac cgatcgcacc
     5161 cacgccgaga cccgcgccgc cgaggcgatg gagatgtgcg acgcgctcgg ccagcccagc
     5221 acgcgcgcgt tcgtcgagac cgtgctcgcg acctggcgct gcctgcgcgg cgacttcgcc
     5281 gaggccgagc gccactccga cgtcgcgctc cggctcgccg ccgaccaggg catgccgcac
     5341 tggcacgccc aggcccagat cacgcgcggc tgggcgatcg ccggtctggg tcgccccgcg
     5401 aagggcgccg cgatcgcccg cgccgggatc aacgcgctca ccggcatcgg ctcgaaggcc
     5461 ggcatgacgt tctactgggg cgcgctcgcc gaggccgagc tcgccgccgg ccgcctcgac
     5521 caggcgacca ccgcgctcga ggaggccgtg cgctacatgg cgctcagcgg cgagcgcatc
     5581 caccagcccg gcctcgcgct gatcgaggcc aggatcgcgg tcgccgccgg ccagcgcgac
     5641 gccgccgacg acgccctgac ccgcgcgctc tccgtcgcct accagcagga ggccggcgag
     5701 gtcgcccgcc gcgccaagga gctacgggcg cagctcgaca gcaccccgca cagccccgcg
     5761 ccgcgcaaca cgcccggctc gatccccgcg gtgcccgaca tcctgtcgta aacgacggcc
     5821 gaccggatca tagccagtct ggatcgagct tgaggtacga gtcctcaccg tcgcggcaca
     5881 gcgcggcgag atgatcgatg cgcggcagct cggtgcggat ccagtactgc gcggcggcga
     5941 gcttggcgcg gtagtagccg gcgtcgcgcg aggcgcggcc gcgcggcagc ggccgggcga
     6001 gcgcctcctg ggcgatcgcg gcgaggtcga gccactgcca ggcgatcacg gtggtcgcga
     6061 acaggtcgag gtagtcggtc gcgtgcagca ccatcgcct
//
LOCUS       DASVOR010000039         2445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_146544,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000039 DASVOR010000000
VERSION     DASVOR010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146544"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..333)
                     /locus_tag="VF516_00840"
     CDS             complement(<1..333)
                     /locus_tag="VF516_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8106234.1"
                     /translation="MIRNLDGAAKTDNETKYHCYRMCRAAGLPERGWHILRHGFGTHA
                     AMFGVNPWKLMLWMGHKRIDETMLYVHFAEAHLRPLPEPLLSAQRGHDDPDKRVIAML
                     GARRLCLPR"
     gene            complement(330..521)
                     /locus_tag="VF516_00845"
     CDS             complement(330..521)
                     /locus_tag="VF516_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106235.1"
                     /translation="MKLSIRALDWKRDVDLVAGTLTVNRQTGLGQTTTPKGRTRRTVP
                     MTDTLVSALKALDTIRTGS"
     gene            672..1040
                     /locus_tag="VF516_00850"
     CDS             672..1040
                     /locus_tag="VF516_00850"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8106236.1"
                     /translation="MVSLDRSPRLDLVFHALAHPARRAIVRQLSGGERNLSELASPLK
                     MTFPAATKHVRVLERAKLVRRRIVGREHLCRLQAEPLREAMLWTESFREHWEARFQAL
                     DSLLDEMQVEEQSRSKRKTP"
     gene            1037..1519
                     /locus_tag="VF516_00855"
     CDS             1037..1519
                     /locus_tag="VF516_00855"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8106237.1"
                     /translation="MTTGLQITTPTDTTIVLTRNFKAPRRLVWEAMTTPDKMKRWMLP
                     PPGLTLNVCEVEPRLGGSLKLAWKSADADPVMTLHGEWTEFSPHERMVHTETMKLGSG
                     ETIASLVETHEFTENGGVTLMRITQTYKSKEDRDGALASGMDQGMEACYQQLDALVAQ
                     "
     gene            complement(1915..>2445)
                     /locus_tag="VF516_00860"
     CDS             complement(1915..>2445)
                     /locus_tag="VF516_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106238.1"
                     /translation="HWSNVTDEYGPHSQAVASALMTLDNTRWLEQVGQPWQERVVDRP
                     DDVTIIRSWDESLTIFDEDPRYNANGVLEAPCARVDPILERYSERKAWWQKARDEAKR
                     YTVLRGWAPQTLAREQRDLIFEYLYEFVSMLLAEILAAPEAECTYFREQLPWFHAGHF
                     PCGWDGDWPVGRMRVY"
BASE COUNT          431 a          828 c          762 g          424 t
ORIGIN      
        1 acgcggaagg cacaacctgc gcgcgccaag catcgcgatg acccgcttgt cggggtcatc
       61 gtggcctcgt tgcgcactca ggagcggctc cgggagcggc ctcaggtgag cctccgcgaa
      121 gtggacgtag agcatcgtct cgtcgatccg cttgtggccc atccacagca tcagcttcca
      181 cgggttcacc ccgaacatcg ccgcatgggt gccgaagccg tgccgcagga tgtgccagcc
      241 gcgctccggt aggcccgccg cccggcacat ccggtagcag tggtacttgg tctcgttgtc
      301 ggtcttcgcc gcgccgtcga ggttccggat cacgaaccgg tgcggatcgt gtccagcgcc
      361 ttgagcgccg agaccagcgt gtccgtcatc ggcaccgtgc gccgcgttcg gcccttcggc
      421 gtggtcgtct gaccgagccc cgtctgccgg ttcaccgtga gtgtcccggc gaccaggtcg
      481 acgtcacgct tccagtcgag cgcgcggatg gaaagcttca tggacctcgc gaccaagctg
      541 atcgaggagc agatgggcat caccgagcag ctgccgcttc cgctcgacga gaagaagtag
      601 gcccgcgctc cgccgctggg cgccgtggtt ttcatgtttg acatatgaaa ccaatcggta
      661 tactaaacca tatggtttca cttgaccggt cgccgcggct cgaccttgtc ttccacgcgc
      721 tggcccaccc agccaggcgg gcgatcgttc gtcagctgtc tggtggcgag cgcaacctca
      781 gcgagctcgc gtcgccgctc aagatgacgt tccccgcggc gaccaagcac gtgcgcgtgc
      841 tggagcgagc aaagctcgtt cgccggcgca tcgtcggccg cgagcacctc tgccgcctgc
      901 aagccgaacc actcagggaa gcgatgctgt ggaccgaatc gttccgcgag cattgggaag
      961 cgcgcttcca agcactcgat tcacttctcg acgaaatgca ggtcgaagag cagtcccgtt
     1021 ccaaaaggaa aacaccatga ccaccggcct ccagatcacc acgcccacgg acaccaccat
     1081 cgtcctcact cgcaacttca aggcgccgcg ccgtcttgtc tgggaggcga tgaccacgcc
     1141 ggacaagatg aagcgctgga tgctgccacc gcccgggttg acgctgaatg tgtgcgaagt
     1201 cgagccgcgc cttggcggtt cgctaaagct cgcgtggaag agtgccgacg cagatcccgt
     1261 catgacgctg cacggtgagt ggaccgagtt ctcgccgcac gagcggatgg ttcataccga
     1321 aacgatgaag ctcggctcgg gcgaaacgat cgcctcgctc gtcgagacac acgaattcac
     1381 cgagaacggc ggcgtcacgc ttatgcgcat cacgcagacc tacaagtcca aggaggatcg
     1441 cgacggtgcg ctcgcgtccg gcatggatca gggcatggaa gcctgctacc agcagctcga
     1501 cgcgctggtc gctcagtagc gagcggacat ccctgcgagc tcgtctccgc gttcgaccga
     1561 gtccaccgcg atcatggcat caccgcgcgt gagttctgcg ccgcgctcgc gctgtccgag
     1621 cgcacgttcc gctcgtggca ggaccggccg gcgaagccgc caccgccgcc gtcaccgcca
     1681 aagccgccga gtccgccgag taacgatagg cgcgtccacg acgtgcgcgc ggcgatcgcg
     1741 acgttggccg cacactggta cccgtccccg agctgaccgc actcttgccc ggctcgctcg
     1801 cgcctacacg tccggcggcg agctgccggc tcgaccagga gcactgcgca cgcccgccgt
     1861 ggatatccgc gggcgcgacg gctcgtcctc agatacgccg cccgctagct gccatcagta
     1921 cactcgcatg cgtccgactg gccagtcgcc atcccatccg cacggaaaat ggccggcgtg
     1981 gaaccaagga agttgctcgc ggaagtacgt gcactcggct tcgggggcgg cgaggatctc
     2041 ggcgagcagc atcgagacga actcgtagag atactcgaat atcaggtctc gctgttctcg
     2101 agctagagtc tgcggagccc agccgcgcaa tacggtatat cgtttggctt cgtcgcgagc
     2161 cttctgccac cacgccttcc gttccgagta tcgttccagg atggggtcga cacgcgcgca
     2221 aggagcctcc aggacgccgt tggcgttata ccggggatcc tcgtcgaaga tggtcagcga
     2281 ctcgtcccag gatcggatga tggtgacatc atctggacgg tcgacgacgc gctcttgcca
     2341 aggctggccg acctgctcca gccatcgtgt gttgtcgagc gtcatgagcg cgctggctac
     2401 cgcttggctg tgcggcccgt actcatccgt cacgttgctc cagtg
//
LOCUS       DASVOR010000040         5344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_234254,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000040 DASVOR010000000
VERSION     DASVOR010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234254"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /locus_tag="VF516_00865"
     CDS             <1..1462
                     /locus_tag="VF516_00865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX8106239.1"
                     /translation="TGGTATAEAATTQAATTQAAAAGAAASTGTPFYFGINASPALLS
                     VGYIVGFNVAAVIFAGSVLNRWISVPLFSVFGDATAIMTDKHISLAQALAGANALDAA
                     DLYHKFVTRYLGVGGMLVGGLWSLYKLRKSLLGGITAGLDAYKKRRAGGQESVLRTEH
                     DMPMNIILILIAASVLPLFFIFWHFTGSAWVSAVMAVVMVIAGFLFSAVASYMAGLVG
                     SSNNPVSGITISTILVSALLLLGLGLKTPAGPIAAVLIGGVVCCAAAIGGDNLQDLKC
                     GQLVGSTPWKQQVMQILGVVVAAFAMAPVLNLLNDAYHIGSKALPAPQANLMGTVAFG
                     VFEGGLPKTIIGIGAAIAVVVIVIDSLLQRSGSKIRTPVMAFAVGVYLPFDLNVSIFL
                     GGVISWLVSRALDRAEASPERRGEVERSGLLAAAGFITGEALLGVALAIPVVLQSPLT
                     VAGGKFESFAPPALAFVVLVMFLLYKLALRKEDKPA"
     gene            1625..2812
                     /locus_tag="VF516_00870"
     CDS             1625..2812
                     /locus_tag="VF516_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX8106240.1"
                     /translation="MTRVLVLGFALAAVACGGNSFPSLCATQVPPPPGCATPCDPSPS
                     AASSCPTGLHCSGDGKCDTVCTATGGQCGDNYACTSDGRCVANGNGNGGNDTPDACPS
                     AHFQAMKTTPSIELLIDRSGSMLHDFADNRTTDPTKQKFTIEQTALVGAQGVVTLLQG
                     SVFFGASMFPSDKCPGLFKTPDRKANNEADIAALLSAHVPDPNANTPTAQAIAGAADD
                     FMANPAPKGSPPVILLSTDGLPNTCTDSTLSAQARADTVAAAKNAFAKGIRLFLLVEG
                     NQFDAKFAQDLANAGQGIVAGQPAATVYTATDLKSLSDAFNAIIGGVLSCDLQLTGQV
                     DPTAGQNGTVTLNNMTLNYGTDWTLDANGIVIHLLGTACDKLKSSQNPTVDATFACGA
                     VIF"
     gene            complement(2846..3316)
                     /locus_tag="VF516_00875"
     CDS             complement(2846..3316)
                     /locus_tag="VF516_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8106241.1"
                     /translation="MIRRAEVADHPWIIATGAEAYRDLGDYTRILPSWLEQPGVLAWI
                     DHDLQGRGRAFAMLGFYMEGQAPTAEVVADLLALAVLPAFQHRGIGSKLLTHVIEVAE
                     RVAPSSRITTLRLTVAETNIAAQRLYARAGFRVVDGSATYDRGQRALRMVRPLV"
     gene            complement(3335..3697)
                     /locus_tag="VF516_00880"
     CDS             complement(3335..3697)
                     /locus_tag="VF516_00880"
                     /inference="COORDINATES: protein motif:HMM:NF016924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8106242.1"
                     /translation="MSTLAVVEEILRETAVPMSVREIVERAGARLPSKSKTPDTVVAR
                     DLSMDIKKKGDTSLFARTSPGRYTLRTLAGNVLAADGAAALRAASSMSALPQRKPAAA
                     APPPRARDSDVQGGGFAG"
     gene            3995..4795
                     /locus_tag="VF516_00885"
     CDS             3995..4795
                     /locus_tag="VF516_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEX8106243.1"
                     /translation="MPSTRALIHAPWSASKVSTALRCPRLFHFKYVQKLAEPEVMPET
                     RIGKAIHKALEQTLMGAPLAEATGATRAELPTEIEQLRFDALAAGIPAFTSRIGQFRR
                     RRRVARQLVEYSLAVREDLSATQFYSGDAYYRGVIDLGFLFEDDALALVDHKTGQRIP
                     GTSIADQLEGYAVLAAAAFRHVRSFWLGLHWIAERAVEWSTPVPPETIIDRLVPDLFD
                     NIEAAALAIDDGPRPNPGSWCDRCSYRELCPASRELRYEPVEYEDDED"
     gene            4782..>5344
                     /locus_tag="VF516_00890"
     CDS             4782..>5344
                     /locus_tag="VF516_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106244.1"
                     /translation="MTKTDERLALVERQRRFEWRLAREIQTQAAARFVAGKTHDLLNL
                     IQIVQLASLELERRCTADAGEFIADLRKAADDAGHQLAELMAVARPEEIIVPGAPVGA
                     AVTAAVETVRAASTIEVDLHLAVSPEVATRCSAEELAHVIYGLTLDAATAEAGDGAGD
                     AQSALEHGETEGGSRAPQAIELFVRERT"
BASE COUNT          787 a         1899 c         1866 g          792 t
ORIGIN      
        1 cacgggcggg acggcgacgg ccgaggccgc gaccacccag gccgcgacca cccaggccgc
       61 ggcggccggc gccgcggcga gcaccgggac gccgttctac ttcgggatca acgcatcgcc
      121 ggccctgctg tcggtcggct acatcgtcgg gttcaacgtc gcggcggtga tcttcgccgg
      181 ctcggtgctc aaccgctgga tctcggtgcc gctgttctcg gtgttcggcg acgccaccgc
      241 gatcatgacc gacaagcaca tctcgctcgc ccaggcgctg gccggcgcca acgcgctcga
      301 tgccgcggac ctctaccaca agttcgtgac ccgctacctc ggcgtcggcg gcatgctggt
      361 cggcggcctg tggtcgctgt acaagctacg caagtcgctg ctcggcggca tcaccgcggg
      421 cctcgacgcc tacaagaagc gccgcgccgg cggccaggag tccgtgctcc gcaccgagca
      481 cgacatgccg atgaacatca tcctgatcct gatcgcggcg tcggtgctgc cgctgttctt
      541 catcttctgg cacttcaccg gctcggcgtg ggtcagcgcg gtgatggcgg tggtcatggt
      601 gatcgccggc ttcctgttct cggcggtcgc gtcgtacatg gccggtctgg tcggctcgtc
      661 gaacaacccg gtctcgggca tcacgatctc gacgatcctg gtgtcggcgc tgctgctgct
      721 cggcctcggg ctgaagacac cggccggccc gatcgccgcg gtgctgatcg gcggcgtggt
      781 gtgctgcgcc gcggcgatcg gcggcgacaa cctgcaggac ctcaagtgcg gccagctggt
      841 cggctcgacg ccgtggaagc agcaggtgat gcagatcctc ggcgtggtgg tcgcggcgtt
      901 cgcgatggcg ccggtgctga acctgctcaa cgacgcctac cacatcggca gcaaggcgct
      961 gccggcgccg caggccaacc tgatgggcac cgtggcgttc ggcgtgttcg agggcggcct
     1021 gcccaagacg atcatcggga tcggcgcggc gatcgccgtc gtggtgatcg tgatcgacag
     1081 cctgctgcag cgcagcggct cgaagatccg cacgccggtc atggcgttcg ccgtcggcgt
     1141 ctacctgccg ttcgatctca acgtgtcgat cttcctcggc ggcgtgatct cgtggctggt
     1201 cagccgggcg ctggaccgcg ccgaggcgtc gcccgagcgc cggggcgagg tcgagcgcag
     1261 cggccttttg gcagcggcgg gcttcatcac cggcgaggcg ctgctcggcg tcgccctggc
     1321 gatcccggtc gtgctgcagt caccgctcac cgtcgccggc ggcaagttcg agagcttcgc
     1381 gccgcccgcg ctggcgttcg tcgtcctcgt gatgttcctg ctctacaagc tcgcgcttcg
     1441 caaggaagac aagccggcgt agccggcggg ctcgagctgc cacgcgcggg tgcaagtgca
     1501 ggttcttgca cagccgtgag tggggctaac tccctcaggg tcgagagtct ccacccctta
     1561 gtgcgcggga tccgcggggc gggccgctgg cttttgcggc gcggcgcttg cttcgtgccc
     1621 acgcatgacg cgcgtcctag tccttggctt cgccctggcg gcggtggctt gcggcggtaa
     1681 cagcttcccc agcctgtgcg ccacccaggt gcccccaccc ccggggtgcg cgacaccttg
     1741 cgatccctcc cccagcgcgg ccagcagctg cccgaccggt ctgcactgct cgggcgacgg
     1801 caagtgcgac acggtctgca ccgcgaccgg cggccagtgc ggcgacaact acgcctgcac
     1861 gagcgacggt cgctgcgtcg ccaacggcaa cggcaacggc ggcaacgaca cgcccgacgc
     1921 gtgtccgtcg gcccacttcc aggcgatgaa gacgacgccc tcgatcgagc tgctgatcga
     1981 tcgcagcggt tcgatgctgc acgacttcgc cgacaaccgc acgaccgacc cgaccaagca
     2041 gaagttcacc atcgagcaga ccgcgctcgt gggcgcacag ggcgtggtca ccctgctgca
     2101 gggctcggtg ttcttcgggg cgtcgatgtt tccgagcgac aagtgtccag gcctgttcaa
     2161 gacccccgac cgcaaggcga acaacgaggc ggacatcgcg gcgctgctca gcgcgcatgt
     2221 cccggatccc aacgccaaca ccccgacggc tcaggccatc gccggggcgg ccgacgattt
     2281 catggcgaac ccggccccca agggctcgcc gccggtgatc ttgctgtcca cggatggctt
     2341 gccgaacacc tgcacggaca gcacgctgag cgcccaggcc agggcggaca ccgtcgccgc
     2401 cgcgaagaat gcgttcgcca agggcatccg gttgttcctc ctcgtggaag gcaaccagtt
     2461 cgacgccaag ttcgcgcagg acctcgcgaa cgccggccag ggcatcgtgg ccgggcagcc
     2521 ggccgcgacc gtctacacgg cgaccgacct gaagtcgctg tccgacgcgt tcaacgcgat
     2581 catcgggggc gtgctgtcct gcgatctcca gctcaccggc caggtcgacc cgaccgcggg
     2641 tcagaacggc acggtcacgc tgaacaacat gaccctgaac tacggcaccg actggacgct
     2701 cgatgccaac ggcatcgtca tccacctgct cggcacggcc tgcgacaagc tcaagagctc
     2761 gcagaacccc acggtcgacg cgacgttcgc ctgcggcgcg gtgatcttct agcgcccgcc
     2821 cgggcagccg gcgccggccc gccgctcaga ccagcgggcg caccatgcgc agcgcgcgct
     2881 ggccgcggtc gtaggtcgcc gagccgtcga cgacccggaa tccggcgcgc gcgtacagcc
     2941 gctgcgcggc gatgttggtc tcggcgacgg tgagccgcag cgtggtgatc cggctcgacg
     3001 gggcgacccg ctcggcgacc tcgatgacgt gggtcagcag cttggagccg atgccgcggt
     3061 gctgaaaggc cggcaggacg gccagggcga gcagatcggc gacgacctcg gcggtggggg
     3121 cctgaccctc catgtagaag ccgagcatcg caaacgcccg gccgcggccc tggaggtcgt
     3181 ggtcgatcca ggccagcacg cccggctgct cgagccagga cggcaggatg cgggtgtagt
     3241 caccgaggtc gcgatacgcc tcggcgccgg tcgcgatgat ccatggatga tccgcgacct
     3301 cggcgcgacg gatcacattg ccaccgcggc gagctcagcc ggcgaagccg ccgccctgca
     3361 cgtcgctgtc gcgcgcgcga ggcgggggag cggcggccgc cggcttgcgc tgcggcagcg
     3421 cgctcatcga cgacgcagcg cgcagcgcgg ccgcgccgtc ggccgccagg acattgcccg
     3481 ccagcgtgcg cagcgtgtac cggccgggcg acgtgcgggc gaacagcgac gtgtcgccct
     3541 ttttcttgat gtccatcgag aggtcgcgcg cgacgaccgt atctggagtt ttcgacttgc
     3601 tcggcaggcg cgcgccggcg cgctccacga tctcgcggac cgacatcgga accgctgtct
     3661 cgcgcaggat ctcttccacc acagcaagcg tactcatgac cgtcgtgcct ccctcgagaa
     3721 aagacgttgc gttcagagcg tcgagacggg gccatcctag cgccgcgagg ctccggaacc
     3781 agtcttgaca tccctttttt ttcggagccc aactgcgcga acaaccaacc ggaaccggcc
     3841 cctggggatt ttgcttgcat ccggccggtc cggccgcgtg gtggcgcgac gggcggatcg
     3901 cagcggatcg cagcggtccg gggcacgtgc gcctccgggt gcggcgcggc ccgcgcacgg
     3961 ccggagcacg ggttttcggc tacgctgtgg cggcttgccg tcgacgcgcg cgctcatcca
     4021 cgccccgtgg tcggcctcca aggtgtcgac cgcgctgcgc tgcccgcggc tgtttcattt
     4081 caagtacgtg cagaagctcg ccgagcccga ggtcatgccg gagacccgga tcggcaaggc
     4141 gatccacaag gcgctcgagc agacgctgat gggagcgccg ctcgccgagg ccaccggggc
     4201 gacccgcgcc gagctaccga ccgagatcga gcagctgcgg ttcgacgcgc tggccgccgg
     4261 gatcccggcg ttcaccagcc ggatcggcca gttccggcgg cgacgccggg tcgcgcgcca
     4321 gctggtcgag tactcgctcg cggtgcgcga ggacctgtcg gcgacccagt tctacagcgg
     4381 cgacgcgtac taccgcgggg tgatcgacct cgggttcctg ttcgaggacg acgcgctcgc
     4441 gctggtcgac cacaagaccg gacagcgtat cccgggtacg tcgatcgccg accagctcga
     4501 gggctacgcc gtgctcgcag cggcggcgtt ccgccacgtc cggtcgttct ggctcggcct
     4561 gcactggatc gccgagcgcg ccgtcgagtg gagcacgccg gtgccgcccg agaccatcat
     4621 cgatcggctg gtcccggacc tgttcgacaa catcgaggcc gcggcgctgg cgatcgacga
     4681 cgggccgcgc cccaaccccg gatcgtggtg cgatcggtgc agctaccgcg agctgtgccc
     4741 ggccagccgc gagctacgat acgagccggt cgagtacgaa gatgacgaag actgacgaac
     4801 ggctcgccct cgtcgagcgc cagcgccggt tcgagtggcg gctggcgcgc gagatccaga
     4861 cccaggccgc cgcgcggttc gtcgccggca agacccacga cctgctcaac ctgatccaga
     4921 tcgtgcagct cgcgtcgctc gagctcgagc ggcggtgcac cgccgacgcc ggcgagttca
     4981 tcgccgacct gcgcaaggcg gccgacgacg ccgggcacca gctcgccgag ctgatggcgg
     5041 tggcgcggcc cgaggagatc atcgtgcccg gtgcgccggt cggcgcggcg gtgaccgcgg
     5101 cggtcgagac cgtgcgggcg gcctcgacga tcgaggtcga cctccacctg gcggtcagcc
     5161 ccgaggtcgc gacccgatgc tccgcggagg agctcgccca cgtgatctac ggcctgacgc
     5221 tcgacgcggc gaccgccgag gccggggatg gggccggcga cgcgcagagc gcgctcgagc
     5281 atggcgagac ggagggcggg agcagagccc cccaggcgat cgaactgttc gtccgtgagc
     5341 ggac
//
LOCUS       DASVOR010000041         9445 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_234258,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000041 DASVOR010000000
VERSION     DASVOR010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9445
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234258"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="VF516_00895"
     CDS             complement(<1..1581)
                     /locus_tag="VF516_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX8106245.1"
                     /translation="MTDPATARSEATGAQRAMGRHAHHAPHGKELAALSLAALGVVYG
                     DIGTSPLYAMSECLSASPAKEHAIAPAANGMYDPAQVLGVLSLFFWALMLVVVFKYLV
                     FVLRADNKGEGGILALAALVEQTDRSPRRKLSVPVLLALFGAGLLYGDGVITPAVSVL
                     GAVEGISEQNPAFSELVVPISAAILIGLFWVQRVGTSRIGSVFGWVMLLWFIAIGVAG
                     GRFILHRPEVLLAVSPHHAIAFLAHNGLHGFLLLGSVVLCITGGEALYADMGHFGRTP
                     IRVAWSVVVFPGLLLNYFGQGALFLEEGARVSNGFYDLVSGTPFLLPMVILATMAAII
                     ASQALISGAFSLTHQAVQLGYLPRVSVVHTSHKHEGQIYIPEVNWLLMVCCVALVFAF
                     RSSSNLAAAYGIAVTGTMAITSFLYFLVVRRNWGYPMYTALALFIPFIAIDLAFFSAN
                     TEKIAAGGWFPLAVGVGAFAMMTTWWRGRMELSKQMESGTIPDELFLADIAETPLPRV
                     SGTAVFMTSGTGGMPNVLL"
     gene            1712..1894
                     /locus_tag="VF516_00900"
     CDS             1712..1894
                     /locus_tag="VF516_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106246.1"
                     /translation="MTRRTFGGRARACGRVGPVLGPVIVAALAALAASCDNLSPKVSG
                     VSSHDRGSGPIVIGHF"
     gene            complement(1930..3570)
                     /locus_tag="VF516_00905"
     CDS             complement(1930..3570)
                     /locus_tag="VF516_00905"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8106247.1"
                     /translation="MTAGPAGAGRLRRSVGFYGLMFISLGSIIGSGWLLGALNAAKLA
                     GPASVLSWIIAGVLLALLALTYAELSAAYPVAGGAARYPYYSHGPIAGFTAGWAAWLQ
                     ATFISPIEVIAAITYVNSVDWVHTNFNMLHTTGPDAGLLNGTGLVVALVLMVVFTTIN
                     LAGAKFLSDSNVIVVVWKTAVPFIAAGLIGGLQFNSANFHAGGGFMPFGFHGVFAALT
                     GGVVFSMQGFEQAGQIAGEARNPKKDLARAIVAAMLIGAVLYSVLQFVMIGALKPSDI
                     ASGWSKPLGGDPSAYGAWYSLALAVGAVWLAKVLLVDAVISPAGTGVVYLGTSARLSY
                     ALGEEREMPGALASTSPKGVPVVSILVAFVVGCLALGPFKSWNALVSVVTSATAIMYA
                     FGPLSLAALHKLDAHRARAYRAPLPKILLPAAFCSANLIIYWGGFDTTWKLVLAMVFG
                     LGLFSFGAWRKRTGARARLRNAIWMVPWFGGHVVIGALGRYGGGYEILPNWFDIVLVI
                     AFALAMFHFALRLTLSPDAVVAAIAKDAHQIDYETPTT"
     gene            3623..3796
                     /locus_tag="VF516_00910"
     CDS             3623..3796
                     /locus_tag="VF516_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106248.1"
                     /translation="MVVVVCAYRLKASSRGRALLAVRENEVAAEAVPRAGSLNNRPGV
                     LALGRGVIPFSPP"
     gene            3793..4329
                     /locus_tag="VF516_00915"
     CDS             3793..4329
                     /locus_tag="VF516_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106249.1"
                     /translation="MKLLVAVALVLAACGSNPRSSTTPTPPKEGQGTGSAATVALPDV
                     PFADLDQDQRIAFMKQKVMPVMKPIFQNHDAKDFANFGCVTCHGEQAKQGHFDMPNPK
                     LPKLDFNDLSEFKKDDLEWMGKQVLPTMGKVLNLPLYSKDNPKGFGCLNCHTLKGKDD
                     HNDHDHDRDHDHDRGHDK"
     gene            4544..4837
                     /locus_tag="VF516_00920"
     CDS             4544..4837
                     /locus_tag="VF516_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106250.1"
                     /translation="MAENTKMPPNQDQRQRMPSGKPSDAKPQKSGGPDPSGAGPDRDR
                     QGVNNPGKQVPEIDPDRARPDRNKPVPGKQGPGQGSMDDDLDDEGLPPPIDRE"
     gene            complement(4876..6057)
                     /locus_tag="VF516_00925"
     CDS             complement(4876..6057)
                     /locus_tag="VF516_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106251.1"
                     /translation="MSATTFRNGPLVLYVDDERGNRVVFEQSLNSEFNLRTASSAAEA
                     LDVLEKHEVAAVVSDMRMPSMSGEELLRIVKERHPEVIRMVVTAYADVEPILRAINEG
                     LVARYIVKPWIRTELVQVLRWAIEAWTFTRDSAALHRRLLETERLATLGSIASMLVHD
                     LKQPLMSLLVNVEHLRDLANAAPLLRDALLQAPIPSTQRARLVELVDDLDPVTTDLKT
                     SGTHLSELISGLRELGKPRDPRNPTTATDPLPIVRHAMTVCQELAIPARASIGYDGPG
                     ELPRVRMSATELTQVLINVVANGAQAVAARGVPHGKVSIVARPDADMLELQIRDDGIG
                     MAPDVLSRVGTPFFTTRPDGTGLGLAQCQRLVGTAGGRFRIESERGIGTTVTIILPIA
                     A"
     gene            complement(6085..9144)
                     /locus_tag="VF516_00930"
     CDS             complement(6085..9144)
                     /locus_tag="VF516_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106252.1"
                     /translation="MREVASRVFHIFFRPLIAKGIALDQMVEGTGVSLARLRKRNERI
                     DWADLCALLGRLQPHFTEDEFVEVGRSYFRSPALRFAFVIGRALLTPMGFYRFINKPR
                     IGAGNQMFTCVVPSHREISDTECEVELTLPEEFEVCWPFFLVSKGNFVEMPRLLGYPA
                     AAVELERIPRGGRYHITIRQRTRLVTRIYRTLTWPFTVRAAARELRDAHESLIERYHE
                     IEDAKAQLDRQATQLRTAHTVNDMVQRDLDLARTLATVARALVDEAGFAWAEIRLRPA
                     DPEAAAAARTVRYGGGDSDHEPPLSRALEVQGTQIGELEVAPRTGANRAEREELLAFI
                     APTLAMALQNALSYQALADYRSNLERLVDQRTGELRQASDQLAGTVVELREAQGARER
                     FFGNITHEIRTPLSLILLAAADIERRAGHVLDERARRNLGAVSDGANKLVRLVDELLL
                     LAAGQEDKLRTNPERTDLGALLGAVVAAWEPAAEAAGLTLTGPAIASLVANVDPVAIE
                     RVASNLMSNAVKYTPRGGSIEVELADEPDGLRFSVLDTGIGMSEDLQSRLFGRFERDQ
                     PGRQRATGTGLGLSVVKQLVEAHGGTISARPRPTGTGSELRVVLPPGIVIRDSEPRPI
                     TVARLGLAPAPATGEPTTAVLAPSGISGGTILLAEDDVRLAEVIGRGLADEYTVVIAH
                     DGASALELVTRHQPQMLITDVDMPGINGIELSRRFRELTGDKLAPIIVLSAMLDLGTR
                     MAGLEAGAVDYVTKPFDPLELRARIRSQFRMRDLAVRLQRAEQLSAMGILTAGLAHEL
                     RNPANGIVNAVGPIVDLLPRELVQPETAVGQLLEVLVACAEQIGFLSRQLLGFRKGVV
                     DLDLRWVPVRELIHRTVALTHAGDVEVRVDVASDGSVKCAPPLVTQALTNLLENAIHA
                     AGAGGWVQLRASAGNDRLSIELSDSGGGVPVALRERVFEPFFTTKPVGIGTGLGLPLA
                     RDIVHRHGGLLEIRERGARTCFVIELPNYSALEAANRPGMMASPRLR"
     gene            complement(9141..>9445)
                     /locus_tag="VF516_00935"
     CDS             complement(9141..>9445)
                     /locus_tag="VF516_00935"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX8106253.1"
                     /translation="VVGAALAAAGHAAADVGLFAVHQGTPWLRKVTQAACGLVNARAV
                     DSFPTTGYLFGASIPLVLELAQRRGLVAAGDLVVLHGGGVGSTYSAIALRWGAAVA"
BASE COUNT         1544 a         3280 c         3315 g         1306 t
ORIGIN      
        1 gagcagcacg ttgggcatgc cgccggtgcc cgaggtcatg aacaccgcgg tgcccgacac
       61 ccgcggcagc ggggtctcgg cgatgtcggc gaggaacagc tcgtcgggga tcgtcccgct
      121 ctccatctgc ttggacagct ccatgcggcc gcgccaccac gtcgtcatca tggcgaacgc
      181 gccgacgccg accgcgagcg ggaaccagcc gccggccgcg atcttctcgg tgttggccga
      241 gaagaacgcc agatcgatcg cgatgaacgg gatgaacagc gcgagcgcgg tgtacatcgg
      301 gtagccccag ttgcggcgca cgaccaggaa gtagaggaac gacgtgatcg ccatcgtgcc
      361 ggtcaccgcg atgccgtagg cggccgccag gttcgagctc gaccggaacg cgaacaccag
      421 cgcgacgcag cagaccatga gcagccagtt gacctcgggg atgtagatct ggccctcgtg
      481 cttgtgcgag gtgtgcacga cgctgacccg cggcaggtag ccgagctgga ccgcctgatg
      541 ggtcagcgag aacgcgcccg agatcagcgc ctgcgacgcg atgatcgccg ccatggtcgc
      601 gaggatcacc atcggcaaca ggaacggcgt gccgctgacc aggtcgtaga acccgttgga
      661 cacccgcgcg ccctcctcga ggaacagcgc gccctggccg aagtagttga gcaacaggcc
      721 ggggaacacc acgaccgacc acgcgacccg gatcggcgtg cggccgaagt ggcccatgtc
      781 ggcgtacagc gcctcgccgc cggtgatgca cagcacgacc gagccgagca gcaggaagcc
      841 gtgcaggccg ttgtgcgcga ggaacgcgat cgcgtggtgc ggcgacaccg cgagcaggac
      901 ctcggggcgg tgcaggatga agcggccgcc ggcgacgccg atcgcgatga accacagcag
      961 catcacccag ccgaacaccg agccgatccg ggacgtgccg acccgctgga cccagaacag
     1021 gccgatcagg atcgcggccg agatcgggac gaccagctcg ctgaacgccg ggttctgctc
     1081 cgagattccc tcgaccgcgc cgagcaccga gaccgccggg gtgatcacgc cgtcgccgta
     1141 gagcaggccg gcgccgaaca gcgcgagcag gaccgggacc gacagcttgc gccgcgggct
     1201 gcggtcggtc tgctcgacca gcgcggcgag tgcgaggatc ccgccctcgc ccttgttgtc
     1261 ggcgcgcagc acgaacacca ggtacttgaa caccaccacc agcatcaggg cccagaagaa
     1321 cagcgacagc acgccgagca cctgcgccgg gtcgtacatc ccgttcgccg ccggcgcgat
     1381 cgcgtgctcc ttggcgggcg acgccgacag gcactcgctc atcgcgtaga gcggcgaggt
     1441 gccgatgtcg ccgtagacca cgccgagcgc ggcgagcgac agcgcggcga gctccttgcc
     1501 gtgcggcgcg tggtgcgcgt gccggcccat tgcgcgctgt gcgccggttg cctccgagcg
     1561 tgcggtggcg gggtctgtca tgtccgcggg caatcgggcc catctcctgg tagcacggcg
     1621 agatccctct gagctacgtg gcgggcgcgc gtgttgccgc gccaacagcg aacagttgac
     1681 acgacggcac gttacgggct aggccgcagg agtgacgcga cgaacgttcg ggggccgtgc
     1741 gcgcgcgtgc ggacgggtcg gccccgtgct cggcccggtg atcgtcgctg ccctcgcggc
     1801 gctcgccgcg tcgtgcgaca acctgtcgcc caaggtctcc ggggtcagct cgcacgaccg
     1861 cggcagcggg ccgatcgtga tcggccactt ctagacgctc aaagtctggg agctggcgag
     1921 cgggcgcgtc taggtggtcg gtgtctcgta gtcgatctgg tgcgcgtctt tcgcgatcgc
     1981 tgccaccacg gcatcgggcg acagcgtcag acggagcgcg aaatggaaca tcgcgagcgc
     2041 gaaggcgatg acgaggacga tgtcgaacca gttcggcagg atctcgtagc cgccaccgta
     2101 acggcccagg gcgccaatca cgacatgtcc gccgaaccac ggtaccatcc agatcgcgtt
     2161 tcgcagcctg gcgcgggcgc cggtgcgctt gcgccacgcg ccgaacgaga acagcccgag
     2221 gccgaacacc atcgcgagca cgagcttcca cgtggtatcg aagccgcccc agtagatgat
     2281 gaggttggcg gagcagaacg ccgccgggag caggatcttg ggcagcggcg cccggtaggc
     2341 gcgtgcgcga tgagcgtcga gcttgtggag cgccgcgagc gacagcggtc cgaacgcgta
     2401 catgatcgcc gtggccgagg tgaccacgga gaccagcgcg ttccagctct tgaacggtcc
     2461 gagggccagg catccgacga cgaacgcgac gaggatcgat acgaccggca cgcccttggg
     2521 gctggtgctg gccagcgcgc ccggcatctc ccgctcttcg ccgagcgcgt acgagagccg
     2581 tgccgaggtt ccgaggtaca cgacaccggt gcccgccggt gagatgaccg cgtcgacgag
     2641 caggaccttc gcgagccaga cggcgccgac cgcgagcgca agcgagtacc atgcaccgta
     2701 cgcagatgga tcgccgccga gcggcttgga ccagccgctg gcgatgtcgc tcggcttgag
     2761 cgcaccgatc atcacgaact ggagaaccga gtagagcacc gcgccgatca acatcgcagc
     2821 gacgatcgcg cgcgcgaggt ccttcttggg atttcgggct tcgcccgcga tctggccggc
     2881 ttgctcgaac ccctgcatcg agaacacgac gccgccggtc agcgcggcga acacgccgtg
     2941 gaagccgaac ggcatgaagc cgccgccagc gtgaaagttg gcggagttga actgcaggcc
     3001 gccgatgagg ccggcggcaa tgaacggcac ggccgtcttc cagaccacga cgatgacatt
     3061 gctgtcggag agaaacttcg cacccgcgag gttgatcgtc gtgaacacga ccatcaggac
     3121 gagcgcgacg acgagcccgg tcccgttgag cagaccggca tcggggccgg tggtgtggag
     3181 catgttgaag ttcgtgtgca cccagtcgac gctgttcaca tacgtgatcg ccgcgatcac
     3241 ttcgatcggt gaaatgaacg tggcctggag ccacgccgcc cagccggcgg tgaagccggc
     3301 gatcgggccg tgcgaatagt aggggtatcg cgccgcgccg cccgcgacgg gatacgcggc
     3361 gctcagctcg gcgtaggtga gcgcgagcag cgcgagcagc accccggcga tgatccacga
     3421 caggaccgac gccggaccgg cgagctttgc ggcgttgagc gcgccgagca gccagcctga
     3481 cccgatgatc gagccgagcg agatgaacat gaggccgtag aaaccaacgg atcggcgtag
     3541 ccgacccgct ccggcaggtc cagcggtcat tggaggctac acatgtgcga tcacctgggc
     3601 acggacgata caccgaacgc gggtggtcgt cgtggtgtgc gcgtaccggc tcaaggcctc
     3661 gagccgcggc cgagcgctgc tcgcggtgcg cgagaacgag gtcgccgccg aggcggttcc
     3721 gcgcgccgga tctctcaaca accggccggg tgtgcttgct ctcggccggg gcgtcattcc
     3781 attctcgccg ccatgaagct gctcgtcgct gtcgctctcg tcctcgctgc ctgtggctcg
     3841 aacccgcggt cctccacgac tcccacgccc cccaaggagg gccagggcac cggctcggcc
     3901 gccaccgtcg cgctgcccga cgtgccgttc gccgacctcg atcaggatca gcgcatcgcg
     3961 ttcatgaagc agaaggtcat gccggtgatg aagccgatct tccagaatca cgacgcgaag
     4021 gactttgcca acttcggctg cgtgacgtgc cacggcgagc aggccaagca ggggcacttc
     4081 gacatgccca accccaagct ccccaagctc gacttcaacg acctgagcga gttcaagaag
     4141 gatgacctcg agtggatggg caagcaggtc ctgccgacga tgggcaaggt cctcaacctg
     4201 ccgctgtact cgaaggacaa ccccaagggg ttcggctgcc tcaactgcca cacgctcaag
     4261 ggcaaggacg accacaacga ccacgaccac gatcgcgacc acgaccacga tcgcggtcac
     4321 gacaagtgat cggctgagcg gtccgagcag ttctgaccaa caaagaccgg gccaccgagg
     4381 gagatcacgt acgagcgatc gtctcgcgtg cgcgcgcgcc cgcgggtgga atccgcgcgg
     4441 tcggccgccg cgaatgtcgt ggaaaccgtg ggacgcggtc gcgggtcgtc ggggcacatg
     4501 cgttgcacct ctgtcgggaa cacccccaac acgggagtac gacatggccg agaacacgaa
     4561 gatgccgccg aaccaggacc agcgtcagcg catgccgtcg ggcaagccct cggacgccaa
     4621 gccgcagaaa tcaggtggcc ctgacccgtc gggcgctggc ccggatcgcg accgccaggg
     4681 cgtgaacaac ccgggcaagc aggtgcccga gatcgatccg gatcgcgccc gcccggatcg
     4741 caacaagccc gttcccggca agcagggccc gggccagggc tcgatggacg acgatctcga
     4801 cgacgagggc ctcccgccgc cgatcgaccg cgagtagtcg tcgcgatcgc cgcggtcgcc
     4861 gccggccatt gctgctcagg cggcgatcgg caggatgatc gtcaccgtcg tcccgatccc
     4921 gcgctcgctc tcgatgcgga accggccgcc ggccgtgccg accaggcgct ggcattgcgc
     4981 caggcccagg ccggtgccgt cgggccgcgt cgtgaagaac ggcgtgccga cccggctcag
     5041 cacgtcgggc gccatgccga tgccgtcgtc gcggatctgc agctccagca tgtcggcgtc
     5101 gggccgcgcc acgatcgaga ccttgccgtg cggcacgccg cgcgcggcga ccgcctgggc
     5161 gccgttggcg accacgttga tcaggacctg ggtgagctcg gtggccgaca tccggacccg
     5221 cggcagctcg ccggggccgt cgtagccgat cgacgcccgg gccgggatcg cgagctcctg
     5281 gcacaccgtc atcgcatggc ggacgatcgg cagcgggtcg gtcgcggtgg tcgggttccg
     5341 cggatcgcgc ggcttgccga gctcgcgcag ccccgagatc agctcgctga ggtgcgtgcc
     5401 cgaggtcttg agatccgtgg tgaccggatc gaggtcgtcg accagctcga ccagccgggc
     5461 gcgctgcgtg gacgggatcg gcgcctggag cagtgcgtcg cggagcagcg gcgcggcgtt
     5521 ggcgagatcg cgcaggtgct cgacgttgac cagcagcgac atcagcggct gcttgaggtc
     5581 gtgcaccagc atgctcgcga tgctaccgag cgtggcgagc cgctcggtct ccagcagccg
     5641 gcgatgcagc gcggcggagt cgcgggtgaa cgtccaggcc tcgatcgccc agcgaagcac
     5701 ctgaaccagc tcggtgcgga tccagggctt gacgatgtag cgagcgacca gcccctcgtt
     5761 gatcgcgcgc aggatcggct cgacgtcggc gtacgcggtc accaccatgc ggatgacctc
     5821 ggggtgccgc tctttcacga tccggagcag ctcctcgccg ctcatcgacg gcatccgcat
     5881 atcggatacc accgcggcaa cctcatgctt ctcgagcaca tcgagggctt ccgcggcgct
     5941 tgacgctgtg cgtaggttga actccgagtt gaggctctgc tcgaagacga cgcggttacc
     6001 ccgttcgtcg tccacgtaca ggaccagcgg gccattgcga aaggtcgtcg cagacatgat
     6061 cgtcgcgcag cggctaccca ccggttagcg tagccgaggc gatgccatca tacccggtcg
     6121 attggcggct tccagcgcag aataattagg aagctctatt acaaaacagg tccgagctcc
     6181 acgttcgcgg atctccagca atccgccgtg gcggtggacg atgtcgcggg ccagaggaag
     6241 cccgagcccg gtcccgatcc cgaccggctt tgtcgtgaaa aatggctcga atacccgctc
     6301 tcgcagcgcg actgggaccc cgccgccgct gtcggacagc tcgatcgaga gccggtcgtt
     6361 gccggcgctg gcccggagct ggacccagcc tcccgccccg gcggcgtgga tcgcattttc
     6421 cagcaggttg gtcagcgcct gcgtcaccag gggtggcgcg cacttgaccg agccgtcgga
     6481 cgccacgtcg acccggacct cgacatctcc ggcgtgtgtg agggcgacgg tacgatggat
     6541 cagctcgcgc accggcaccc accgtaggtc cagatcgacg acccccttgc ggaacccgag
     6601 cagctggcgc gacaggaacc cgatctgctc ggcgcaggcg accagcacct ccagcagctg
     6661 gccgaccgcg gtctcgggct gcacgagctc gcggggcagc aggtcgacga tcggccccac
     6721 cgcgttgacg atgccgttgg ccgggttgcg cagctcgtgg gcgagccccg ccgtcaggat
     6781 gcccatcgcc gacagctgct cggcgcgctg cagccggacc gccaggtctc gcatccggaa
     6841 ctgcgaccgg atccgggccc gcagctcgag cggatcgaac ggcttggtca cgtagtcgac
     6901 cgcgccggcc tccaggccgg ccatccgggt gccgaggtcg agcatggccg acagcacgat
     6961 gatcggcgcg agcttgtctc cggtcagctc gcggaaccgc cgcgacagct cgatgccgtt
     7021 gatcccgggc atgtcgacgt cggtgatcag catctgcggc tggtgcctgg tgaccagctc
     7081 cagcgcggac gccccgtcgt gcgcgatcac cacggtgtac tcgtcggcga gcccgcggcc
     7141 gatcacctcg gcgagccgga cgtcgtcctc ggcgagcagg atcgtgccgc ccgagatccc
     7201 gctcggcgcc agcacggcgg tcgtcggctc gccggtcgcc ggcgccgggg ccagcccgag
     7261 ccgggcgacc gtgatcgggc gcggctcgct gtcgcggatc acgatcccgg gcggcagcac
     7321 gacccggagc tcgctgccgg tgccggtcgg gcgcggccgc gcgctgatcg tgccgccgtg
     7381 ggcctcgacc agctgcttga cgaccgacag gcccagcccg gtcccggtcg cgcgctggcg
     7441 gccgggctgg tcgcgctcga accgcccgaa cagccggctc tgcaggtcct cgctcatccc
     7501 gatcccggtg tcgagcaccg agaaccgcag gccgtcgggc tcgtcggcga gctcgacctc
     7561 gatcgatccg ccgcgcgggg tgtacttgac cgcgttcgac atcaggttgg acgcgacgcg
     7621 ctcgatcgcg accgggtcga cgttggcgac cagcgacgcg atcgccggcc cggtcagggt
     7681 caggccggcg gcctcggcgg cgggctccca cgcggcgacc accgcaccga gcagcgcccc
     7741 gaggtcggtg cgctcggggt tggtccgcag cttgtcctcc tggccggcgg cgagcagcag
     7801 cagctcgtcg accagccgca ccagcttgtt cgcgccatcg ctcaccgccc cgaggttcct
     7861 gcgggcgcgc tcgtcgagca cgtgaccggc gcggcgctcg atgtcggcgg cggcgagcag
     7921 gatcagcgac agcggcgtgc ggatctcgtg ggtgatgttg ccgaagaacc gctcgcgcgc
     7981 gccctgggcc tcgcgcagct cgaccaccgt gccggcgagc tggtcgctgg cctggcgcag
     8041 ctcgccggtg cgctgatcga ccagccgctc gaggttcgac cggtagtcgg cgagcgcctg
     8101 gtagctcagc gcgttctgca gcgccatcgc cagggtcggc gcgatgaacg cgagcagctc
     8161 ctcgcgctcg gcgcggttgg ccccggtccg cggtgcgacc tcgagctcgc cgatctgcgt
     8221 gccctggacc tcgagcgcgc ggctcagcgg cggctcgtga tcgctgtcgc cgccgccgta
     8281 ccgcaccgtg cgcgcggccg cggccgcctc cggatcggcc gggcgcagcc ggatctcggc
     8341 ccacgcgaag ccggcctcgt cgaccagcgc gcgggcgacc gtggcgagcg tgcgcgccag
     8401 atcgaggtcg cgctggacca tgtcgttgac ggtgtgggcg gtgcgcagct gggtcgcctg
     8461 gcggtcgagc tgggccttgg cgtcctcgat ctcgtggtag cgctcgatca gcgactcgtg
     8521 ggcgtcgcgc agctcgcgcg ccgcggcccg cacggtgaac ggccaggtca gcgtgcggta
     8581 gatccgggtg accagccggg tccgctgccg gatcgtgatg tggtagcgac cgccgcgcgg
     8641 gatccgctcg agctcgaccg cggccgccgg gtagccgagc aggcgcggca tctccacgaa
     8701 gttgcccttc gagaccagga agaacggcca gcacacctcg aactcctcgg gcagggtcag
     8761 ctcgacctcg cactcggtgt cggagatctc gcggtgggac ggcaccacgc aggtgaacat
     8821 ctggttgccg gcgccgatcc gcggcttgtt gatgaagcgg tagaacccca tcggggtcag
     8881 cagcgcccgc ccgatcacga acgcgaaccg cagcgccggc gaccggaagt acgaccggcc
     8941 gacctcgacg aactcgtcct cggtgaagtg cggctgcagc cggccgagga gcgcgcacag
     9001 gtcggcccag tcgatccgct cgttgcgctt gcgcagccgg gcgagcgaca ccccggtgcc
     9061 ctcgaccatc tggtcgagcg cgatgccctt ggcgatcagc gggcgaaaaa agatgtggaa
     9121 gaccctgctt gcgacttctc tcatgccacc gcagctcccc agcgtagcgc gatcgcgctg
     9181 taggtcgagc ccacgccgcc gccgtgcagg accacgaggt cgcccgccgc gacgaggccg
     9241 cggcgctgcg cgagctccag cacgagcggg atgctggcgc cgaacaggta gccggtggtc
     9301 gggaagctgt cgaccgcgcg ggcgttgacc agcccgcacg ccgcctgggt caccttgcgc
     9361 agccacggcg tgccctggtg gaccgcgaac agcccgacgt ccgcggcggc gtggcccgcg
     9421 gcggcgagcg ccgcgccgac cacct
//
LOCUS       DASVOR010000042         2017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_292780,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000042 DASVOR010000000
VERSION     DASVOR010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292780"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..268)
                     /locus_tag="VF516_00940"
     CDS             complement(<1..268)
                     /locus_tag="VF516_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106254.1"
                     /translation="MASSAPSPSRLLAIIELQNAIAAAAMNADEVMHIVVDRARGLLS
                     AATTLIALVEGDDLVLRTVIGAMAQLAGSRVPRTAGLAGRCVAER"
     gene            527..1108
                     /locus_tag="VF516_00945"
     CDS             527..1108
                     /locus_tag="VF516_00945"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEX8106255.1"
                     /translation="MSGRVRIGTCANSADAALVRSVFSARGIGVVIGAEHHASLFAPL
                     GASFLSLDIWVASEDAEEAAALLRDLREAKAPGPGSEAARPEGEHDDENDDDEEDLER
                     ERKVRSEQKRRTALAILLGCFVTFGTAHMVTGAWARGIVLAVIELVGIRHLVHGERIG
                     TAIVLAAVVTDLIGGLWRLRAEPNLPVARLRRP"
     gene            1244..>2017
                     /locus_tag="VF516_00950"
     CDS             1244..>2017
                     /locus_tag="VF516_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106256.1"
                     /translation="MATIECNETGGGDPDGSAGGAGGRVPRGEDEWLWGWDPTPGIVS
                     VWAEPDGRAFVWRRDPSTGRLVRDDVRFRPWLLVTALNDLAHLGPRLRPEHNVQDSRD
                     ARDRPARLITYRELAGPGELRYLVRADDMATLVTAILHGAARRLGRPLGHLRDLPDDQ
                     VLALAPEEQYLVATGRTYFRGLAFDDLRRLQVDLETTGLDAARDRIFLIAVRDPGGAT
                     ALLEAAGDGPDAEADLIARLVACIRACDPDVIENHNLHGF"
BASE COUNT          268 a          765 c          709 g          275 t
ORIGIN      
        1 ggcgctcggc gacgcagcgg ccggccagcc ccgcggtacg cggcacgcgg ctgcccgcga
       61 gctgcgccat cgcgccgatc acggtgcgca gcaccaggtc gtcgccctcg accagcgcga
      121 tcaacgtggt cgccgccgac agcaggccgc gggcccggtc gacaacgatg tgcatcacct
      181 cgtccgcgtt catcgcggcg gcggcgatcg cgttctggag ctcgatgatc gcgagcagtc
      241 gcgacgggct gggcgccgag cttgccaccg cgcacatcat acagccgtcg gcggaatccg
      301 ctggcgccgc gccgagatct tcggcggtcc tttgctgcaa cgccgcgccg gcgcggagtg
      361 agctgagcca ctttcgcgaa accgctgctg cgattcgcgc cacagcacgt acccggctgt
      421 agggctttcg cctaccctcg atggccccga cggcgctccg gcaggccggc aacgcacgaa
      481 ttgtgcatcc gccggagcgc caccgggccg tgctaagaac tgctccgtga gtggccgcgt
      541 ccgcatcgga acctgcgcca attccgccga tgccgccctg gtccggtcgg tgttctccgc
      601 gcgcggcatc ggcgtcgtga tcggcgccga gcaccacgcc agcctgttcg cgccgctcgg
      661 cgccagcttc ttgtcgctcg acatctgggt cgcatcggag gacgccgagg aggccgccgc
      721 gctgctgcgc gacctgcgcg aagccaaggc gccgggaccc gggtccgaag cggctcggcc
      781 ggagggcgag cacgacgacg agaacgacga cgacgaggag gatctggagc gcgagcgcaa
      841 ggtgcgcagc gagcagaagc ggcgcacggc cctggcgatc ctgctcggct gcttcgtcac
      901 gttcggcacc gcgcacatgg tcaccggcgc gtgggcccgc ggcatcgtgc tcgcggtgat
      961 cgagctggtc ggcatccgcc acctcgtgca cggcgagcgg atcggcaccg cgatcgtgct
     1021 cgccgcggtc gtcaccgacc tgatcggcgg gctgtggcgg ctgcgtgcgg agccgaacct
     1081 gcccgtcgcg cgcctccgcc ggccgtagcg caccctggca ggtcgctccg cggcgaccag
     1141 agatctgcac cacgtcatgc cggcgctcag gcaagcttca tgatgctatg cgtggcgtag
     1201 cgccccggcg gccgtgccgc cgggtatgcc cgagacagtg ccggtggcga ccatcgagtg
     1261 caacgagacg gggggtgggg accccgacgg ctccgccggc ggggcggggg gcagagtccc
     1321 ccgtggagaa gatgaatggc tgtggggctg ggacccgact cccggcatcg tctcggtgtg
     1381 ggccgagccc gacggccgcg cgttcgtgtg gcggcgcgat ccatcgaccg gccggctcgt
     1441 gcgcgacgat gtccggttcc ggccgtggct gctggtcacc gcgctcaacg acctcgcgca
     1501 cctcggcccg cgcctgcggc ccgagcacaa cgtccaggac tcccgcgatg cccgcgaccg
     1561 gccggcgcgc ctgatcacct accgcgagct cgccggcccg ggcgagctgc gctacctggt
     1621 ccgcgccgac gacatggcga cgctggtcac cgcgatcctg cacggcgcgg cgcgccggct
     1681 cggccgcccg ctcggccacc tgcgcgacct ccccgacgac caggtcctcg cgctcgcccc
     1741 cgaggagcag tacctggtcg cgaccggccg cacctacttc cgcggccttg cattcgacga
     1801 tctccgccgg ctccaggtcg atctcgagac caccggcctc gacgccgcgc gcgaccggat
     1861 cttcctgatc gccgtgcgcg atcccggcgg cgccaccgcg ctgctcgagg ccgcgggcga
     1921 tggccccgac gccgaggccg atctgatcgc gcgcctggtc gcctgcatcc gcgcctgcga
     1981 tcccgacgtg atcgagaacc acaacctgca cggcttc
//
LOCUS       DASVOR010000043         4399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_555782,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000043 DASVOR010000000
VERSION     DASVOR010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555782"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..943)
                     /locus_tag="VF516_00955"
     CDS             complement(341..943)
                     /locus_tag="VF516_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEX8106257.1"
                     /translation="MQRLSRTLIQLNLATREHHVAADAPWLELLVPTVTRRQYAEQLI
                     KVYGFEAPLEAALRYTPGLSTLVDLRSRVRSGLIVQDLMRLGLGAGRIAALGQRFVTF
                     SSTLEALGWLYVVERATLLHGAIRRYLTLRVPELCGATSYLSAYDGVTGDRWSDLGNA
                     LETVAHAPPAKHQLIRAANQGFLAFCEWFEGGTALRSVGT"
     gene            complement(1216..2670)
                     /locus_tag="VF516_00960"
     CDS             complement(1216..2670)
                     /locus_tag="VF516_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8106258.1"
                     /translation="MNGSVLVVDDDIDTAQLLRDGLRRRGLSVNSVHSARECLGRLAT
                     EPVDVVVTDIYMSDMTGLQLCEQIRQRYQDVLPIVITGQSTLDNAIGAIRAGAYDFIL
                     KPVKLDTLAIAVGRALGHLALRRELKQLRAAVGDEPPDGIAGTSPAIRETIDMVHRVA
                     DSDATVLITGESGTGKELVARAIHRLSPRRDQPFVAVNCAAMPAPLLESELFGHVRGA
                     FTDARTARSGLFIQAGTGTIFLDEIGEMPAEMQVKLLRVLQEREVRPVGGDENIPFHA
                     RVLAATNRNLEAEVEQRRFREDLFYRINVVTLHVPPLRARSGDILLLAQHFVRRIAQR
                     ISKPVKGFSSAAARMLLDYDWPGNVRELENCIERAVALCRLDEVTVDDLPTKVQEFHV
                     TRIVIPAGPTASPDAMITMEEMERRYVRQVLEAVRGNKTHAARILGIDRRSLYRRLET
                     PYQASRPEAAPEAAQPPPAAVEEQPEQPKTGESE"
     gene            3330..3683
                     /gene="queF"
                     /locus_tag="VF516_00965"
     CDS             3330..3683
                     /gene="queF"
                     /locus_tag="VF516_00965"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEX8106259.1"
                     /translation="MTTRPSKQLEIFENPRPGRDYRIAFECPEFTCLCPMTGQPDFAT
                     ILIDYVPDRLCVELKSLKLYLWSYRDEGAFHEAVTNRICDDLVAAIAPRSITVTGRFW
                     VRGGISTTVTVTHHG"
     gene            complement(3667..>4399)
                     /locus_tag="VF516_00970"
     CDS             complement(3667..>4399)
                     /locus_tag="VF516_00970"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEX8106260.1"
                     /translation="PTMSLADGQHAKHERLCELLALSPRDHLLEIGCGWGGMAIHAAR
                     TRGCRVTAITVSRQQHELATARVAAAGLADRIDIRYRDYRELDGRTTYDKLVSIEMLE
                     AVGYEYLPAYFAIAARLLRPGGRFALQSITMPDARFAAYRRRVDWMQTYVFPGSLIPS
                     LGAIAAAAGPAGFRIERADDIGPDYAATLAAWRARFLAALPAVTALGFDEPFIRTWLM
                     YLAFSEAAFTERTLGDHQLLLSRGA"
BASE COUNT          681 a         1517 c         1547 g          654 t
ORIGIN      
        1 cgccggctgg ggcggcgacc gcgttggcgc ccagcccacc gcccatgacc tcggcggccg
       61 cgcgcttcgc ggtcgatcca ccgccgccga tcatcaagcc gcgccaggac accgcggccc
      121 ccaagccggc cgagaagcgc cccgagcaga tcttcacgtt ctgagcgcgc acgccacgct
      181 gggcggatga tctaccaccc ggctaccgcc cggctaccgc ccggccctac cgcccggcgc
      241 atgcgctgta ccggccttcc tcgatctgct tcgctcggtc cgcgatgacg tcgcccgcac
      301 cccctggtgc cggcgacgtt gtcgttttcg gccgagccgg ttaggtaccg accgaccgga
      361 gcgcggtgcc accctcgaac cactcgcaga acgccaggaa gccctggttc gccgcgcgga
      421 tgagctggtg cttggccggc ggtgcgtgtg cgaccgtctc cagcgcgtta ccgaggtcgc
      481 tccagcggtc gccggtgacg ccgtcgtacg ccgacaggta gctggtcgcg ccgcagagct
      541 cggggacccg cagggtcagg tagcgacgga tcgcgccgtg gagcaaggtc gcgcgctcga
      601 cgacgtacag ccagccgagc gcctcgagcg tggacgagaa cgtcacgaag cgctggccga
      661 gcgcggcgat ccgcccggcg ccgaggccga gccgcatcag gtcctggacg atcagaccgg
      721 agcggacccg cgagcgcaga tcgaccagcg tgctcagccc gggcgtgtaa cgcagcgcgg
      781 cctcgagcgg cgcctcgaag ccgtagacct tgatgagctg ctcggcgtac tgccgcctgg
      841 tgacggtcgg gaccagcagc tccagccacg gtgcgtcggc cgcgacgtga tgctcccggg
      901 tcgcgaggtt gagctggatg agcgtccgcg agagccgttg catcgaccgc caaagagcac
      961 cctgcaagca acatgcacgg acgcgttgcc atcgcgcgtc gcgcagccgg ccgcgcgtcc
     1021 ggccgtcgcg attcgcgcac ggctcacgcg atcctcatga tccgtcaggc gcgcggggcg
     1081 gtttcggtcc ggcggctgga accgcatgcc ggcggtcggc gtccgtgcgg gcaacgcttg
     1141 cctcgcgagg ctgctggcgc gccggctacc ctgtgcgcgg gcgccgcgca cacgtgcctc
     1201 ggcgccacgc cggcgtcatt cgctctcgcc ggtcttcggt tgctcgggtt gctcttcgac
     1261 cgctgccggg ggcggctgcg cggcttccgg cgccgcctcc ggacgcgacg cctggtacgg
     1321 cgtctccagc cggcggtaca gcgagcggcg gtcgatgccg aggatccgcg cggcgtgggt
     1381 cttgttgccc cggaccgcct cgaggacctg gcgcacgtag cgccgctcca tctcctccat
     1441 cgtgatcatc gcgtcgggag acgcggtcgg cccggccggg atcacgatcc gggtgacgtg
     1501 gaactcctgg accttggtcg gcagatcgtc gacggtgacc tcgtcgagcc ggcacagcgc
     1561 gaccgcgcgc tcgatgcagt tctcgagctc gcggacgttg ccgggccagt cgtagtcgag
     1621 cagcatccgc gccgccgcgc tcgagaaccc cttcaccggc ttgctgatgc gctgcgcgat
     1681 ccggcgcacg aagtgctggg cgagcagcag gatgtcgccg ctgcgcgcgc gcagcggggg
     1741 cacgtggagc gtgacgacgt tgatccggta gaacagatcc tcgcggaacc ggcgctgctc
     1801 gacctcggcc tccaggttgc ggttggtggc ggcgagcacg cgggcgtgga acgggatgtt
     1861 ctcgtcgccg ccgaccggcc ggacctcgcg ctcctggagc acgcgcagca gcttgacctg
     1921 catctccgcg ggcatctcgc cgatctcgtc gaggaagatc gtgccggtcc cggcctggat
     1981 gaacagcccg gaccgcgcgg tccgggcatc ggtgaacgcg ccgcggacgt ggccgaacag
     2041 ctcgctctcg aggagcggcg ccggcatcgc cgcgcagttc accgcgacga acggctggtc
     2101 gcggcgcggc gacagccggt ggatcgcacg ggcgaccagc tccttgccgg tgcccgactc
     2161 gccggtgatc agcacggtcg cgtcgctgtc cgccacgcgg tggaccatgt cgatggtctc
     2221 gcggatcgcc gggctggtgc cggcgatgcc atcgggtggc tcgtcgccga cggccgcccg
     2281 cagctgcttg agctcgcggc gcagcgcgag gtgaccgagc gcacggccga ccgcgatcgc
     2341 cagcgtgtcc agcttgaccg gcttcaggat gaagtcgtac gcgccggcgc gtatggcccc
     2401 gatcgcgttg tcgagcgtgc tctgcccggt gatcacgatc ggcaggacgt cctggtaacg
     2461 ctgccggatc tgctcgcaca gctgcagacc cgtcatgtcg gacatgtaga tgtccgtgac
     2521 cacgacgtcg accggttcgg tcgccagccg gccgaggcac tcgcgtgccg agtggacact
     2581 gttgacgctg agcccgcgcc ggcgcaagcc gtcgcgcagc aactgcgccg tgtcgatgtc
     2641 gtcatcgacg acgagaacgc tcccgttcac ggcaaaccct tcgccggtgc ggagtagagg
     2701 acctggatag caaggagacc cgacacctgt gggatttgcg tagcacgtta aaaccgaaaa
     2761 agttgccaaa aaatcaacaa gataccacga gcgcctacgc acagcccgtc gagcgatact
     2821 gccacagggg gccgatacga aaaaatccgt acgagattca ccgacggggg tagcaagcct
     2881 cagcccgatc cgcccggcat taaaaaatgg cctcacccag gaactccgac caactgcgaa
     2941 agacagctcc gaaaataaga acgggcccgg tcggccgggc ccgtttgctc gttccttacc
     3001 cagctgggtc tcagctgagg tctagcggga ggctaggagt gcccctcctt agagcggtga
     3061 ctggtacatt gcatgttctt ctttcctccc ctggcgtgta gccagagccc gacaccaaac
     3121 ggcccgccag cagaattgca gcggttcccg gggtcgggcc ttcgtaatgg gcacagcggc
     3181 ccgtttcgaa gtccacgatt cgtgatggcc ggacggtcat cgcgcgtgtg ctcggcgaca
     3241 tggtcgcaag atcattgtag gcgaagcgtg ggcaaagtgc caggacttga gccagcaggg
     3301 agaggagatc gccgtggtat gcagccccgg tgacgacgag gccgagcaag cagctggaga
     3361 tcttcgagaa cccccggccg ggccgcgact accgcatcgc gttcgagtgc cccgagttca
     3421 cctgcctgtg cccgatgacc gggcagccgg atttcgcaac catcctgatc gattacgtgc
     3481 cggaccggct gtgcgtggag ctcaagagcc tcaagctgta cctgtggagc taccgggacg
     3541 aaggcgcgtt tcacgaggcc gtgaccaacc ggatctgcga tgacctggtc gccgcgatcg
     3601 ccccgcggtc catcaccgtg accggcaggt tctgggtgcg cggcgggatc tccacgaccg
     3661 tgacggtcac gcaccacggc tgagcaggag ctggtgatcg ccgagggtgc gctcggtgaa
     3721 tgctgcctca ctgaacgcca ggtacatgag ccaggtgcgg atgaacggct cgtcgaagcc
     3781 cagcgcggtc acggcgggga gcgcggcgag gaaccgcgcc cgccacgccg ccagggtcgc
     3841 ggcgtagtcg gggccgatgt cgtcggcgcg ctcgatccgg aagcccgcgg gcccggcggc
     3901 ggcggcgatc gcgccgagcg acgggatcag cgatcccggg aacacgtagg tctgcatcca
     3961 gtcgacgcgc cggcggtagg ccgcgaaccg cgcgtcgggc atcgtgatcg actgcagcgc
     4021 gaaccgtccg cccgggcgga gcagccgcgc cgcgatcgcg aagtacgcgg gcaggtactc
     4081 gtagccgacc gcctcgagca tctcgatcga gaccagcttg tcgtaggtcg tgcggccgtc
     4141 gagctcgcgg taatcgcggt accggatgtc gatgcggtcg gccagcccgg cggccgcgac
     4201 ccgcgcggtg gcgagctcgt gctgctggcg cgacacggtg atcgcggtga cccggcatcc
     4261 gcgggtgcgc gcggcgtgga tcgccatgcc gccccagccg cagccgatct cgagcaggtg
     4321 gtcgcgcggc gacagcgcga gcagctcgca cagccgctcg tgcttggcgt gctggccgtc
     4381 ggcgagcgac atcgtgggc
//
LOCUS       DASVOR010000044         3812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_555796,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000044 DASVOR010000000
VERSION     DASVOR010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_555796"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VF516_00975"
     CDS             <1..1146
                     /locus_tag="VF516_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106261.1"
                     /translation="RSRLSHAAALDRAGRSEDARDLAGEIVDDAEQLGYPPLVAEAHL
                     ALGKILLGRHEPAAATTSLARARAVGLEQRMLALAVEAGARQIYVEAIQNGRSSDLLR
                     DAALLEPISRGLVGDHFARPLLLNNIGSVHMADGDRAAARRSFEEAQAALQGVAAPDV
                     ELTCISKNLAMLTPDPAERRRLARSAWEQRRNALGDHHLFTLDALDSYARFEANPDDA
                     YPLVAAACAGYDAYAELVELRLYCHSYRAFLAEYRGDSRTALSAYQDIAHLAERAEAD
                     DVFWGQLATGHSRRLSGDPTGAIAAFSPVFQSYWNSSDWWVRYRAADAALGWGLAERD
                     RHHLTEATRLLRKAAAMYDEIAQHNEETEFRIRAELARRAIGSLATP"
     gene            1462..2541
                     /locus_tag="VF516_00980"
     CDS             1462..2541
                     /locus_tag="VF516_00980"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEX8106262.1"
                     /translation="MNRFPINGLNPGGCKNSQQIALVPESIRKNKGDCNGKTLAVEED
                     LAGSPPKGARLIAKDANDCDLDGATFSVKRMDGKEFEIEIHVLPGHFSSRTGGKTYPG
                     YVFTLPHQETSLCKCSEKKGKCTEADFVAFQQALGIKNDLPFWERKHSRDDVEFVLDA
                     GTIETADTWDPDDIAIIIPSEVHGEDGSTFVDSEQKGWFNIACSGDALAKADLYQIQP
                     HMKDPTGGPPKYYQQRDTALKLITANYCDQDRYTFKGNEIEWDFRDKNGNWPKQPSDR
                     FFEAVWNEHGAVCVSNSRLYHTSTIAFIPNWIYPDDCYNKKTCDDENGFQAALKKVCP
                     GVRPCKLDQNGYLTEGEGYLVSRSH"
     gene            complement(2701..3321)
                     /locus_tag="VF516_00985"
     CDS             complement(2701..3321)
                     /locus_tag="VF516_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8106263.1"
                     /translation="MADEDLDLLDRWISGDTAAGNQLFKRNFTLVYRFFETKTEGEID
                     DLVQETFLACLKSRATFRRQSTFRTYLLAIARHTLFHYWRKRRPSGEPIDFQEVSVAS
                     LSTSVGSRLGAEQERAMLLAALRAMPLDQQLLIELYYWEDLDRDALAAVFDVEPATIG
                     TRLFRARQALREHVERPSGAPAAGAPTVDLDGWMRAQRPGSQPIQP"
     gene            complement(3512..3597)
                     /locus_tag="VF516_00990"
     tRNA            complement(3512..3597)
                     /locus_tag="VF516_00990"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3561..3563),aa:Leu,seq:taa)
BASE COUNT          710 a         1252 c         1225 g          625 t
ORIGIN      
        1 cgctcccgcc tgtcccacgc cgccgcgctc gaccgcgccg gccgctcgga ggacgccagg
       61 gatctcgccg gggagattgt cgacgacgcc gagcagctcg gctacccgcc gctcgtcgcg
      121 gaggcccacc tcgcactggg caagatcctg ctcggccgcc acgagcccgc cgcggccacc
      181 acgtccctgg cccgcgcgcg ggcggtcggc ctcgagcagc gaatgctcgc gctcgcggtc
      241 gaggcaggag cgcgccagat ctacgtcgag gcgatccaga acggccgtag cagcgacctc
      301 ttgcgcgacg ccgccctgct cgagccgatc agccgcggcc tggtcggcga ccacttcgcc
      361 cggccattgc tcctcaacaa catcggcagc gtccacatgg ccgacggcga ccgcgccgcg
      421 gcgcgccggt cattcgagga ggcccaggcc gcgctccagg gcgtcgccgc gcccgacgtc
      481 gagctgacct gcatctccaa gaacctcgcc atgctgactc ccgatccggc cgagcgccgc
      541 aggctcgccc gcagcgcctg ggagcagcgg cgcaacgcgc tcggcgacca ccacctgttc
      601 acgctcgatg cgctcgatag ctacgctcgg ttcgaggcga acccggatga tgcctacccg
      661 ctcgtcgccg cggcctgcgc tggctacgac gcgtatgccg agctggtcga gctgcgcctg
      721 tactgtcaca gctaccgcgc cttcctcgcc gagtatcgcg gcgactcacg taccgccctg
      781 tccgcctacc aggacatcgc ccacctcgcc gagcgcgccg aggcagacga cgtcttctgg
      841 ggccagctcg cgaccggtca cagccgacgc ctctccgggg atccgaccgg cgcgatcgcg
      901 gcattctcac ctgtattcca gtcttactgg aacagttccg actggtgggt tcgctaccgc
      961 gccgccgatg ccgcgctcgg ctggggactc gccgagcgcg accgccatca cctcaccgag
     1021 gcgactcgcc tgctccgcaa ggccgcggcg atgtacgacg agatcgcgca gcacaatgag
     1081 gagaccgagt tccggattcg ggccgagctg gccaggcgcg cgatcggaag tctcgctacc
     1141 ccgtgacgcc cccagattct caggaacggc ttgtcgaaag gtggcactac tcgacacaga
     1201 tacctcgagg atgccatgca cgacaccata cgctcacctc atctcgcgac tcggccactg
     1261 ccgatatgta tattgctctg gatcggatcg atgatctggt ccggatgcgg ctccaggcca
     1321 gctgagccgt ccgcgatggc gcgatcggcg gtcgcgtcgt gtcccgatct cccccagggc
     1381 aagccggccc gaagcactgg catcgcggcc actgttacgg ggagctgtca gacctcgaac
     1441 tgtggcggaa actcccccac ggtgaaccgg tttccgatca atggactcaa tccgggcgga
     1501 tgcaagaact cccagcagat cgccctggtc cccgagagca tccggaagaa caaaggcgac
     1561 tgcaatggca agacgctcgc tgtcgaggag gatctcgccg gttcgccacc caagggtgct
     1621 cggctgatcg caaaagacgc aaacgactgc gatctcgacg gagcgacatt ttcagtcaaa
     1681 cggatggatg gcaaagaatt cgagatcgag atacatgtcc ttccggggca tttctcgtcg
     1741 cgcacaggag gaaagacata ccctggatat gtcttcactc tccctcatca agaaacctcc
     1801 ctctgcaaat gctcggaaaa aaagggcaag tgcactgagg cagactttgt ggcctttcaa
     1861 caggccctgg ggatcaagaa cgatcttccg ttctgggagc gcaagcacag cagagacgat
     1921 gtcgagttcg tgctcgatgc gggtacgatc gaaactgcgg acacctggga tccagacgat
     1981 attgcgatca tcattccgag cgaagttcat ggcgaggatg gctccacgtt cgtcgactca
     2041 gaacagaagg gatggttcaa catcgcctgc tcgggagatg cgttggccaa ggctgacctc
     2101 taccagatcc agccccacat gaaagaccct actggcggcc cccctaagta ctaccagcag
     2161 cgagataccg ccttgaagtt gataactgcc aactactgcg atcaggatcg ctacacattc
     2221 aaaggcaacg aaatcgaatg ggatttcagg gacaagaacg gaaactggcc caaacagcca
     2281 tcggacagat ttttcgaggc agtctggaac gaacacggcg ctgtgtgcgt ttcaaattca
     2341 cgtctgtatc atacttctac gattgcattt attccaaact ggatatatcc agacgattgt
     2401 tataataaga agacctgcga cgacgagaac ggattccagg cggcactgaa gaaagtctgt
     2461 ccaggtgtca gaccttgcaa gctcgaccag aacggttacc tcaccgaggg tgagggttac
     2521 cttgtgagcc gctcacacta gcccggacaa tttcaccacg acgtctcggt cgatcccttc
     2581 cgctcgcttc gctcgcggtg gctcacggcg tgaggccgat cggggcgcac gccggaagcg
     2641 cagtgaggac agtactggcg ccgcgacagt cgcagccggc ggcgtggtga actgtccggg
     2701 ctagggctgg atcggctggc tccccgggcg ctgggcacgc atccagccgt cgaggtcgac
     2761 cgtgggggcg ccggcggcgg gggcgcccga ggggcgctcg acgtgctcgc gcagcgcctg
     2821 gcgggcgcgg aacagccgcg tgccgatcgt cgccggctcg acgtcgaaca cggcggccag
     2881 ggcgtcgcga tcgaggtcct cccagtagta cagctcgatc aggagctgct ggtcgagcgg
     2941 catcgcacgc aacgcggcga gcagcatcgc acgctcctgc tcggcgccga gcctggaacc
     3001 gacgctggtg ctgagcgaag cgaccgacac ctcctggaag tcgatcggtt caccggacgg
     3061 ccgccgcttg cgccagtagt gaaacagcgt atggcgcgcg atcgcgagca ggtaggtacg
     3121 gaaggtgctc tggcggcgga aggtcgcgcg gctcttgagg caggccagga acgtctcctg
     3181 gacaaggtcg tcgatctcgc cctcggtctt ggtctcgaag aaccggtaga ccagcgtgaa
     3241 gttgcgcttg aacagctggt tgccggccgc ggtatcgccg gagatccagc ggtcgaggag
     3301 atcgaggtct tcgtcggcca tggacggtcc catcggctgg gatgatagcc agatcgcgga
     3361 tccggcgcct cggttgcctc cgctgctctg cgtcacgccg gatgcatgga tgggtcgcca
     3421 gcgcattgat gaaacgcgcg tggcagccgc acgacaggcg cagggtcgcg cggacctccg
     3481 cggccatgtg cgacggcaac tcgcacatcg gtgcccaggg ccggactcga accggcacga
     3541 gccttccggc tcacaacatt ttaagtgtcg tgtgtctacc agtttcacca cccgggcggc
     3601 gcagtcagct tacaccggac ggtgtgcgct gacgaatggc gctgccgcgg ggcgatcagg
     3661 gcgttccaga tccgctgccg gcgggcgatt gaaacggctt gagctggatg gtgtcgtcgt
     3721 cgggcggcgg cgctggccgg ggcggaggcg ccggcggggg cggcttgatc tcggggacat
     3781 ccggccggcc cggctcgggg gcggcggagc cg
//
LOCUS       DASVOR010000045         5260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_526601,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000045 DASVOR010000000
VERSION     DASVOR010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5260
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526601"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VF516_00995"
     CDS             <1..354
                     /locus_tag="VF516_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106264.1"
                     /translation="KQVLGRLALTLPVGRARRLTLRSPAPATQFDDGCRWLRGTSLVV
                     HPDGALGFCCMPTSLPSPASRYHLGDGDLPTVLARHQALAQGIMRQWDQLRETPDRPP
                     LCALCNGVLGNVGVA"
     gene            365..1594
                     /locus_tag="VF516_01000"
     CDS             365..1594
                     /locus_tag="VF516_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106265.1"
                     /translation="MKRFHGILGLRWLAIAALLLLPASITAKPEPDRTAALAAALRVN
                     GRPPAALVAQLLDAHDLLIFDDAVHDAHEPWVFYQALVRAPDFHRRVKWIFLETLPVT
                     AQPKLDRYVQSPVQDLTLLERAFQDDYSGTGWPFQDTLDLIEAVRQVNQALPHDERLR
                     CVAVSPPIYWEALHTRADYDRFLDTLDSRDYAMYLTIRRELDGFHSGRKGVFLTNTRH
                     AYNGLRTRDGRTAWNTAAFFHHWDPGKTCSIRIHNLALQITPRAVPAAGPRSAQGLDD
                     YSMAWVRVDGGAWDAAFARNGNAPVAIVLAGTPFAAAPFVGPGALSAAPDLELGELYD
                     ATIFLSPMDRLHESARTVFYFTPAFKRELKRRLGVLNPGGADDLRKTCGVDSVDACVD
                     KLAVPSGVKPARRDSPR"
     gene            complement(1620..2627)
                     /locus_tag="VF516_01005"
     CDS             complement(1620..2627)
                     /locus_tag="VF516_01005"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106266.1"
                     /translation="MSEATADDLASVASTARDASADIAALPKAELHCHLGGVLDPEMV
                     RDLEAAGHGLGIDPAALERCYPVRSISDFVDVYSAFLDGFLRPRDVRLLPLLVQQVRR
                     WNRQRVTYAELFISDILFARDDLAELVALYQLYRDTARAEAAAGLEVALVACVGRGPL
                     DRLARQTERIIALYEAGAICGVSIAGLEELPLAPAGPLFRRLRDTGMGIEIHAGETGG
                     AASVWDALRHGHPHRLGHALAVFDDERLIDAVGEAGIHLEFCPTSNLCLGCIQRIEDH
                     PLPAARRHGLRFSVNTDDPGAFQCSLTSELELVARRFGLSRADLLAIHADTVAAGFGA
                     R"
     gene            complement(2644..3798)
                     /locus_tag="VF516_01010"
     CDS             complement(2644..3798)
                     /locus_tag="VF516_01010"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8106267.1"
                     /translation="MPYVEGRVVHDADSHIFEPPGTAVRHADPGIRDRLAGAVRELNW
                     GPAAEAAVAQQRDPAFRARDAAEIMLRKNQLAVGAVLPEDRPRALDLLGFASQLVFTT
                     SYLAALREFERGDDLDLMYGLALAHNRAIVEFCATDRRLLPVGYLPLGDIDRAIRAAG
                     QAIELGVAALMIPSHPLRARSQSHIGFDPIWARAQDAGVPIVFHVGGEEPMAPVYKVN
                     GLPPVPDFHGGDSNFTSVSYLAIPYAPMQTLGTMIYDGVLDRFPRLRIGVIELGASWV
                     PGWMRALDSGAEAFRKNEDRLQRLSLTPSEFVRRQVRVTPYPHEPVGWIVKNTFPEVM
                     MFSSDYPHVEGGRHPLRRFETSLAECTAAEQRAFYCDNFVDFMGPRLAGA"
     gene            complement(3833..4204)
                     /locus_tag="VF516_01015"
     CDS             complement(3833..4204)
                     /locus_tag="VF516_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106268.1"
                     /translation="MLVEHFAAVLWVTGAITMTPVLQFLAPRQVLAMLYRIELTDEAG
                     LFLARHWGLLAASIGGLLLFAAAHPELRPAVVTAALVEKAGIVAAFLLVRRRPFARGL
                     RMVAAFDAACVVLYATWLATS"
     gene            4491..5114
                     /locus_tag="VF516_01020"
     CDS             4491..5114
                     /locus_tag="VF516_01020"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HEX8106269.1"
                     /translation="MERSQDQFLAAILRGDLTEALKVAKEARSHGLPFFYEGMIARSL
                     VEVGRLWQAGQISVADEHIATAVAQSVLASFYPLFPWAAAGPKGIVACVEGERHELGA
                     RMAADLLVCDGWNVIYVGADVPLAALVELVVKTRSVFVGLSFALTERLPNVRDAIARL
                     RREVADTKLLLGGRGVALDATQARSLGADAIAFSAASAADMIRVWKQ"
BASE COUNT          771 a         1815 c         1921 g          753 t
ORIGIN      
        1 aagcaggtgc tcggccgcct ggcactcacg ctgcccgtgg gccgcgcgcg ccggctcacg
       61 ctgcgcagcc cggcgccggc gacgcagttc gacgacggct gccgctggct acgcggcacg
      121 agcctggtgg tgcaccccga cggcgcgctg ggcttctgct gcatgcccac cagcctgccg
      181 tcgccggcga gccgctacca cctcggcgac ggcgacttgc cgaccgtgct cgcgcggcac
      241 caggcgctcg cgcaggggat catgcgccag tgggaccagc tccgcgagac ccccgatcga
      301 ccccccctgt gcgcgctgtg caacggcgtg ctcggcaacg tcggtgtggc gtgaggtgcg
      361 ggccatgaag cgattccacg ggatcctggg gctgcgctgg ctggcgatcg cagcgctcct
      421 cctcctgcca gcgtcgatca cggccaagcc cgagccggac cgcaccgcgg cgctggccgc
      481 ggcgctgcgc gtgaacgggc gcccgcccgc ggcgctcgtc gcgcagctgc tggatgcgca
      541 cgacctgctg atcttcgacg acgcggtgca cgatgcgcac gagccgtggg tgttctatca
      601 ggcgctggtg cgcgcgcccg acttccaccg ccgggtgaag tggatcttcc tggagacgct
      661 gcccgtgacc gcccagccga agctggaccg ctacgtccag tctccggtgc aggatctgac
      721 gctgctcgag cgcgcgtttc aggatgacta cagcgggacg ggctggccgt tccaggacac
      781 cctggatctg atcgaggccg tccggcaggt gaaccaggcg ctgccgcacg acgagcggct
      841 gcgctgcgtc gcggtgtcgc cgccgatcta ctgggaggcg ctgcacaccc gcgccgacta
      901 cgatcgtttc ctcgatacac tcgacagccg cgactacgcc atgtatctga cgattcgccg
      961 agagctggat ggattccact ccgggcgaaa gggcgtgttc ctcacgaaca cccgccatgc
     1021 ctacaacggc ctccgcaccc gcgacggccg cacggcctgg aacaccgccg cgttctttca
     1081 tcactgggat cctggaaaga cctgctcgat ccggatccac aacctggcgc tgcagatcac
     1141 gcctcgtgcc gtcccggcag ccgggccccg cagcgcacaa ggcctcgatg actacagcat
     1201 ggcgtgggtg cgcgtggacg gcggggcctg ggatgcagcg tttgcgcgaa acggcaatgc
     1261 cccggtcgcc atcgtgctgg cggggacacc gtttgccgcg gcgccgttcg tgggccccgg
     1321 cgccctgtcc gccgcgccgg acctcgagct cggcgagctg tacgatgcga ccatcttcct
     1381 ctctccgatg gatcggctcc acgagtcggc ccgcaccgtt ttctacttca cccctgcgtt
     1441 caagcgcgag ctgaagcggc ggctcggcgt cctgaacccc ggcggcgccg acgacctgcg
     1501 caagacctgc ggcgtggact ccgtggatgc ctgcgtggac aagctcgcgg tgccgtccgg
     1561 cgtgaagccg gcgcggcggg acagcccgag gtgatcacgg cgccggcatg gcggctcggt
     1621 cagcgagcgc cgaagcccgc ggccaccgtg tcggcatgga tcgcaagcag atcggcgcgg
     1681 ctcaggccga agcggcgcgc gacgagctcg agctccgagg tcagcgagca ctggaacgcg
     1741 cccggatcgt cggtgttcac gctgaaccga aggccgtgtc gcctggccgc cggcagcggg
     1801 tggtcctcga tgcgctggat gcagccgagg cacaggttgc tggtcgggca gaactcgagg
     1861 tggatcccgg cctcgccgac cgcgtcgatc aggcgctcgt cgtcgaacac cgccagggcg
     1921 tggccgaggc ggtgcggatg gccgtggcgg agcgcgtccc acaccgaggc cgcgccgccg
     1981 gtctcgccgg cgtggatctc gatccccatc ccggtgtcgc gcagccggcg gaacagcgga
     2041 ccggccggcg cgaggggcag ctcctcgagc ccggcgatcg acacgccgca gatcgcgccg
     2101 gcctcgtaca gcgcgatgat gcgctcggtc tggcgcgcca gccggtcgag cggaccccgg
     2161 ccgacgcagg cgaccagcgc gacctcgagg ccggctgccg cctcggcgcg cgcggtgtcg
     2221 cgatacagct ggtacagcgc gaccagctcc gccagatcgt cgcgcgcgaa caggatgtcg
     2281 ctgatgaaca gctccgcgta ggtcacgcgc tggcggttcc agcgccggac ctgctggacc
     2341 agcagcggca gcagccgcac atctcgcgga cggaggaagc cgtcgaggaa cgccgagtag
     2401 acgtcgacga aatcgctgat cgatcggacc gggtaacagc gctccagcgc ggccgggtcg
     2461 atccccagcc cgtggcccgc ggcctcgagg tctcgcacca tctcgggatc gagcacgccg
     2521 ccgagatggc agtgcagctc ggccttgggc agggcggcga tgtcggcgga cgcatcgcgc
     2581 gcggtcgatg cgacgctcgc gaggtcgtcg gcagtcgctt cgctcacggt tcgagccgtg
     2641 tggtcaggcg ccggccaggc gcggccccat gaagtcgacg aagttgtcgc agtagaacgc
     2701 gcgctgctcg gccgccgtgc actcggcgag gctggtctcg aaccgccgca gcggatggcg
     2761 cccgccctcg acgtgggggt agtccgacga gaacatcatc acctcgggga aggtgttctt
     2821 gacgatccag cccaccggct cgtggggata cggggtcacg cgcacctggc ggcgcacgaa
     2881 ctcgctcggc gtgaggctga gccgctggag ccggtcctcg ttcttgcgga acgcctcggc
     2941 gccggagtcg agcgcgcgca tccagcccgg cacccagctg gcgccgagct cgatgacgcc
     3001 gatgcgcagc cgcgggaagc ggtcgagcac gccgtcgtag atcatcgtcc ccagcgtctg
     3061 catcggggcg taggggatcg ccaggtagga caccgaggtg aagttggagt cgccgccgtg
     3121 gaagtcgggg accggcggca gaccgttgac cttgtagacc ggggccatcg gctcctcgcc
     3181 gccgacgtgg aacacgatcg gcacgccggc gtcctgggcg cgggcccaga tcgggtcgaa
     3241 gccgatgtgg ctctgcgagc gcgcgcgcag cgggtgcgac gggatcatga gggccgcgac
     3301 gccgagctcg atcgcctggc cggcggcccg gatcgcgcgg tcgatgtcgc cgagcgggag
     3361 atagccgacc gggagcaggc ggcggtcggt ggcgcagaac tcgacgatgg cgcggttgtg
     3421 cgcgagcgcc aggccgtaca tcagatcgag atcgtcgccg cgctcgaact cgcgcagcgc
     3481 cgcgaggtag ctggtggtga acacgagctg gctggcgaag ccgagcaggt cgagcgcgcg
     3541 gggccggtcc tcggggagca cggcgcccac ggcgagctgg ttcttgcgca gcatgatctc
     3601 ggccgcgtcg cgcgcgcgga acgccggatc gcgctgctgc gccaccgccg cctccgcggc
     3661 cggcccccag ttcagctcgc gcaccgcccc ggccagccga tcgcggatcc cggggtcggc
     3721 gtggcgcacg gcggtgccgg gcggctcgaa gatgtgggaa tcggcgtcgt gaacgactcg
     3781 gccttcgacg tagggcatcg cgatcctccg gcgagatgct agcgcggatc gatcacgacg
     3841 tggcgagcca ggttgcgtac agcaccacgc acgcggcgtc gaacgcggcc accatgcgca
     3901 gcccgcgcgc gaacgggcgg cggcggacga gcaagaacgc ggcgacgatc cccgccttct
     3961 cgacgagcgc ggcggtgacc accgcaggcc tcagctccgg atgtgcggcc gcgaacagga
     4021 ggaggccgcc gatcgacgcg gcgagcagcc cccagtgccg ggccaggaac agcccggcct
     4081 cgtcggtcag ctcgatgcga tacagcatcg cgagcacctg cctcggcgcg aggaactgca
     4141 ggaccggggt catcgtgatc gctccggtga cccacagcac cgcggcgaag tgttcgacca
     4201 gcatcgcgcc acgctacctg ggtgaccgcg acggatcaat ccctcccggg cgaccgggag
     4261 ctcgacgtcg cgaggggcga gcccgtgggc gactgcggtc gcgatcgcga ggcgttcggc
     4321 ggcagctgcg gccgacaccg cgttggccgg catcgcgtat cgatcagcgc cgtccggtga
     4381 tgcgtcgtga tcgtcgaagt cgtgatcgtc gatccgcgta cgaggacttc gcgttcgccc
     4441 gtgccccctt gattccttga cgcgcgaacc gaggcggcca tgttgtcgag atggagcgat
     4501 cacaggacca gtttctggcc gcgatcctca ggggagatct gacggaggcg ctgaaggtgg
     4561 cgaaggaggc gcgctcgcac gggctgccgt tcttctacga ggggatgatc gcgcgctcgc
     4621 tggtcgaggt cgggcggctg tggcaggccg gacagatctc ggtcgccgac gagcatatcg
     4681 cgacggccgt cgcgcagagc gtgctcgcca gcttctatcc gttgttcccg tgggcggcgg
     4741 ccggtccgaa agggatcgtc gcgtgtgtcg agggcgagcg tcacgagctc ggagcccgga
     4801 tggccgcgga tctgctcgtg tgtgatggct ggaacgtgat ctacgtcggc gcggacgtac
     4861 cgctcgcggc actggtcgag ctcgtcgtga agacccgctc ggtcttcgtg gggctgtcgt
     4921 tcgcgctgac cgagcgcctg ccgaacgtgc gtgacgccat cgcgcggctg cggcgggaag
     4981 ttgcggacac caagctgctg ctcggcggac gcggcgtcgc gctcgacgcc acgcaggcgc
     5041 ggagtctcgg agccgacgcg atcgcctttt ccgcggcgag cgccgccgac atgatccggg
     5101 tgtggaagca atgagcgacg tcgacgtcgt ggggctcctg ctcgatcgcc gcgtcgcgat
     5161 cccggtgctc gtcgtgcgcg atggcatcat cgcgtcgagc aacgaagcgg cacgcgagct
     5221 gctcgatggc gacgtcgacc agttcatcga ggcgctgttc
//
LOCUS       DASVOR010000046         1589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_438904,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000046 DASVOR010000000
VERSION     DASVOR010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438904"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="VF516_01025"
     CDS             <1..1330
                     /locus_tag="VF516_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8106270.1"
                     /translation="KPNLPRGTNGVYADADQAAQAARRAFEHNERTPVAVRIKMVEAM
                     RRAVLANNEALSRHAVEETGLGRYEDKLAKNQLVAEKTPGPEILRAVAYTGDDGLMLT
                     ERAPYGVLLAVTPTTNPTETILCNAIGMVAAGNAVVFNVHPSAAQTCNWCVHLLNEAI
                     QAAGGPRDVVVSIERPTVESAQLLMKHPLVRLVVVTGGPVVVKQAMNSGKKVIAAGPG
                     NPPAVVDETANLDKAADAIVRGASLDNNIVCIAEKEIIAVDAIASELIRRLESKPVLR
                     LDARQVRALEGVILEGDHVNKAWVGKDAGLIAQQIGLSGHGHDLRLLLCEVEEGHPFV
                     QHELLMPVLPLFRVRDAADAVAAALRVEHGFHHTATMHSRNVDHMAAMARMCNCSIFV
                     KNDASYAGLGLGGEGYTSFTIASPTGEGLTTAVHFTRERRCTLKDAFRFV"
     gene            1327..>1589
                     /locus_tag="VF516_01030"
     CDS             1327..>1589
                     /locus_tag="VF516_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX8106271.1"
                     /translation="MIRGPALGVLEVAAIARGVVVADAALKRAPAVLLSSRAVSGGKH
                     LVMLEGGVAEVEEAMAAGAHAAGDLLLDRVELAAADDQVWPMLG"
BASE COUNT          240 a          555 c          590 g          204 t
ORIGIN      
        1 caagcccaac ctgccgcgcg ggaccaacgg cgtgtacgcc gacgccgacc aggcggccca
       61 ggccgcgcgc cgcgcgttcg agcacaacga gcgcacgccg gtcgcggtcc ggatcaagat
      121 ggtcgaggcg atgcgccggg cggtgctcgc caacaacgag gcgctgtcgc gccacgcggt
      181 cgaggagacc ggcctgggcc gctacgagga caagctggcg aagaaccagc tggtcgccga
      241 gaagaccccg ggccccgaga tcctgcgcgc ggtcgcgtac accggcgacg acggcctgat
      301 gctgaccgag cgcgcgccgt acggcgtgct gctcgcggtg acgccgacga ccaacccgac
      361 cgagaccatc ctgtgcaacg cgatcggcat ggtcgcggcc ggcaacgcgg tggtgttcaa
      421 cgtccacccg tcggcggcgc agacctgcaa ctggtgcgtc cacctgctca acgaggcgat
      481 ccaggccgcg ggcgggccgc gcgacgtcgt ggtgtcgatc gagcgcccga cggtcgagag
      541 cgcccagctc ctgatgaagc acccgctggt ccggctggtc gtggtgaccg gcgggccggt
      601 cgtggtcaag caggcgatga actcgggcaa gaaggtgatc gccgccgggc cgggcaaccc
      661 gccggcggtg gtcgacgaga ccgccaacct cgacaaggcg gcggacgcga tcgtgcgcgg
      721 cgcgtcgctc gacaacaaca tcgtgtgcat cgccgagaag gagatcatcg cggtcgacgc
      781 gatcgcgagc gagctgatcc gccggctgga gagcaagccg gtgctgcggc tcgatgcccg
      841 ccaggtccgc gcgctcgagg gcgtgatcct cgagggcgat cacgtcaaca aggcgtgggt
      901 cggcaaggac gccgggctga tcgcccagca gatcggcctg tcgggccacg gccacgacct
      961 ccggctgctg ctgtgcgagg tcgaggaggg ccacccgttc gtccagcacg agctgctgat
     1021 gccggtgctg ccgctgttcc gggtccgcga cgccgccgac gcggtcgccg cggcgctgcg
     1081 ggtcgagcac gggttccacc acaccgcgac catgcactcg cgcaacgtcg accacatggc
     1141 cgcgatggcc cggatgtgca actgcagcat cttcgtcaag aacgacgcgt cgtacgccgg
     1201 cctcggcctg ggcggcgagg gctacacgtc gttcacgatc gcgagcccga ccggcgaggg
     1261 cctgaccacc gcggtgcact tcacgcgcga gcggcgctgc acgctcaagg acgcgttccg
     1321 gttcgtatga tccgcggtcc ggcgctcggc gtgctcgagg tcgctgcgat cgcgcgcggc
     1381 gtggtagtcg ccgatgccgc gctcaagcgc gcgccggcgg tgctgctgtc gtcgcgcgcg
     1441 gtgtcgggcg gcaagcacct ggtcatgctc gagggcggcg tcgccgaggt cgaggaggcg
     1501 atggcggccg gcgcccacgc cgcgggggac ctcttgctcg atcgcgtcga gctcgccgcg
     1561 gccgacgacc aggtctggcc gatgctcgg
//
LOCUS       DASVOR010000047         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205136,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000047 DASVOR010000000
VERSION     DASVOR010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205136"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1033
                     /locus_tag="VF516_01035"
     CDS             <1..1033
                     /locus_tag="VF516_01035"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8106272.1"
                     /translation="AAPQPIAGVTPVPGDFTIKDFAFTSGEKLPEVRLHYVTLGSAKR
                     DAAGRVTNAVVILHGTTGSGTQFFRKQFTEPLFGPGGALDLTRTYVIIPDAIGHGGST
                     KPSDGLHAKFPHYGYVDLVASTHALLVDGLHVDHLRLVMGTSMGCMHAFLWAERWPAM
                     MDAAMGLACLPAAITGRNRVWRKMLIDAIEQDPEWNHGEYTRQPRAAMLGAGQIFALA
                     NTAPLADQKRLATREEADQDVENGERAFTATYDANDLLYAFHASFDYDPAPELDKITA
                     PFLLVNSADDFINPPELGIAEREMKRIKRGRFVLLPATESTHGHGMHSWATFWQGELR
                     KLLDTPPRK"
     gene            complement(1049..1900)
                     /locus_tag="VF516_01040"
     CDS             complement(1049..1900)
                     /locus_tag="VF516_01040"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8106273.1"
                     /translation="MAEDLANQTFIVTGANTGIGKVTAKELARRGVHVILACRSKDKA
                     EAVIEEIRRDTGSTRAEYVPLDLGDLASVRACAEAIAARGIPIHGLINNAGMAGSKGL
                     TKDGFELTWGVNHLGPYLFTRLLLDRVRQAGRARIVNVASASHYNATGIDWDALKQPT
                     KSATGMREYSVSKLSNVLFAKELARRLDGTGVTTYALHPGVVATDVWRRVPGPLRWLI
                     KKFMITPEQGAVASLRCATDPAFAGQTGRYYDVGGTEKEPSKLAGDAELAKTLWTKSA
                     EWTGLPA"
     gene            2008..2472
                     /locus_tag="VF516_01045"
     CDS             2008..2472
                     /locus_tag="VF516_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX8106274.1"
                     /translation="MKTRTITTTVLLAASLAACGGQARAPEPPAAPAASLYDRLGRKD
                     AIALVVKDFVEQRVAKDQRINGFFASADLPGLEMKLVEQICEATGGPCKYTGIYKDMK
                     TTHAGMAIKDADFTALVEDFTASLQQLHVGAAEQQELLAALGKMHDDIVTAK"
     regulatory      2543..2748
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            2749..>2939
                     /locus_tag="VF516_01050"
     CDS             2749..>2939
                     /locus_tag="VF516_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106275.1"
                     /translation="MKDALSALVIPVVGPGRKPGARRTHATVVRARVTWGDMTFRGRS
                     IGRSILWALLGVGLGAAAAS"
BASE COUNT          456 a         1023 c         1046 g          414 t
ORIGIN      
        1 cgcggcgccg cagccgatcg ccggcgtgac cccggtgccc ggcgacttca cgatcaagga
       61 cttcgcgttc accagcggcg agaagctgcc cgaggtccgc ctgcactacg tgacgctggg
      121 cagcgcgaag cgcgatgccg ccggccgggt gaccaacgcg gtcgtgatcc tgcacggcac
      181 caccggctcg ggcacgcagt tcttccgcaa gcagttcacc gagccgctgt tcggccccgg
      241 cggggcgctc gacctgaccc gcacctacgt gatcatcccc gacgccatcg gccacggcgg
      301 gtcgaccaag ccgtcggacg ggctgcacgc gaagttcccg cactacggct acgtcgacct
      361 cgtcgcgtcg acccacgcgc tgctggtcga cgggctccac gtcgatcacc tgcggctggt
      421 gatgggcacg tcgatgggct gcatgcacgc gttcctgtgg gccgagaggt ggccggcgat
      481 gatggacgcc gcgatgggcc tggcctgcct gccggcggcg atcaccgggc gcaaccgggt
      541 gtggcgcaag atgctgatcg acgcgatcga gcaggatccg gaatggaacc acggcgagta
      601 cacccggcag ccgcgcgcgg cgatgctcgg cgccggccag atcttcgcgc tcgccaacac
      661 cgcgccgctc gccgaccaga agcggctggc gacccgcgag gaggccgacc aggacgtcga
      721 gaacggcgag cgcgcgttca ccgcgaccta cgacgccaac gacctgctgt acgcgttcca
      781 tgcctcgttc gactacgacc cggcgccgga gctcgacaag atcaccgcgc cgttcctcct
      841 ggtgaacagc gccgacgact tcatcaaccc gcccgagctc gggatcgccg agcgcgagat
      901 gaagcggatc aagcgcggcc ggttcgtgct gttgccggcg accgagtcga cccacgggca
      961 cggcatgcac agctgggcga cgttctggca gggcgagctg cgcaagctgc tcgacacgcc
     1021 gccgcgcaag taggctgcgg agcgccggtc acgccggcag gccggtccac tcggcgctct
     1081 tggtccacag cgtcttcgcc agctcggcgt cgccggcgag cttgctcggc tccttctccg
     1141 tgccgccgac gtcgtagtag cggccggtct ggccggcgaa cgcggggtcg gtggcgcagc
     1201 gcaggctggc cacggcgccc tgctccgggg tgatcatgaa cttcttgatc agccagcgca
     1261 gcgggccggg cacgcggcgc cacacgtcgg tcgcgaccac gccggggtgg agcgcgtagg
     1321 tcgtgacgcc ggtgccgtcg agccggcgcg ccagctcctt ggcgaacagc acgttggaca
     1381 gcttggacac cgagtactcg cgcatcccgg tcgcgctctt ggtcggctgc ttcagcgcgt
     1441 cccagtcgat gccggtcgcg ttgtagtgcg acgcgctggc gacgttgacg atccgcgccc
     1501 ggccggcctg ccggacccgg tcgagcagca gccgggtgaa caggtagggc ccgaggtggt
     1561 tgacgcccca ggtcagctcg aagccgtcct tggtcaagcc cttcgatccg gccatcccgg
     1621 cgttgttgat caggccgtgg atcgggatgc cgcgcgcggc gatcgcctcg gcgcacgcgc
     1681 gcaccgacgc gaggtcgccg aggtcgaggg ggacgtactc ggcgcgggtg ctgccggtgt
     1741 cgcgccggat ctcctcgatc accgcctcgg ccttgtcctt cgagcggcag gcgaggatga
     1801 cgtggacacc gcggcgcgcg agctccttgg cggtgacctt gccgatgccg gtgttggcgc
     1861 cggtcacgat gaatgtctgg ttggcgagat cttcggccat ggccggcaac atcggggcgg
     1921 acgccgcacg ggtcaagggc gcacggctgc tgtatgcatg gatcccacgg gggctccgcc
     1981 ccgcctcccg cggcgcggaa gggacccatg aagacgagaa cgatcacgac gacggtgctc
     2041 ctggcggcca gcctcgcggc gtgcggcggc caggccaggg ccccggagcc gccggccgcc
     2101 ccggccgcct cgctgtacga ccggctcggc aggaaggacg cgatcgccct ggtggtcaag
     2161 gacttcgtcg agcagcgcgt cgccaaggac cagcggatca acggcttctt tgccagcgcc
     2221 gacctgcccg gcctggagat gaagctggtc gagcagatct gcgaggcgac cggcgggccg
     2281 tgcaagtaca ccggcatcta caaggacatg aagaccaccc acgccggcat ggcgatcaag
     2341 gacgccgact tcaccgcgct ggtcgaggac ttcacggcgt cgctccagca gctccacgtc
     2401 ggcgccgcgg agcagcagga gctgctcgcc gcgctcggca agatgcacga cgacatcgtg
     2461 accgcgaagt agctgtccgc ggggtggagc caaatcaacc attgactcgc gcggggaccg
     2521 cggctaccta cagggccgga ggtccatcga agacgacgca agcgacagct ccgacggaag
     2581 cagggaagcc ggtgcacaat ccggcacagc ccccgctact gtgaccgggg acaacgcggc
     2641 acgggccatc gccgagacac ggggagggtc cagccactgg gcaactggga aggcgccgcc
     2701 taggacgatc cgggagtcag gacacctgtt tccgtttgca cttcgggtgt gaaggacgcg
     2761 ctgtcggcgc tggtcattcc cgtggtcggg cccgggcgca aacctggggc ccgccggacg
     2821 cacgcaacgg tcgtccgggc acgcgtcacg tggggtgaca tgacgttccg cggaagatcc
     2881 atcggtcgat ccattctctg ggcgctcctc ggcgtcggtc tcggcgcggc ggcggcgtc
//
LOCUS       DASVOR010000048         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_292848,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000048 DASVOR010000000
VERSION     DASVOR010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292848"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..620
                     /locus_tag="VF516_01055"
     CDS             153..620
                     /locus_tag="VF516_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8106276.1"
                     /translation="MKLRPELARKGTRRAVVERAGAVELVARAASSMYGPPQGVDRED
                     QLRHRLGARIRALRDARGMTQEMIADRIAVTPKYVSQLECGQRSPSWETLVAIAHHGF
                     EMRLAALVFGIDEDIPAEVQDLNDMLAGRPKETRRDLLQAMELMLRAGAEPKL"
     gene            complement(650..1588)
                     /locus_tag="VF516_01060"
     CDS             complement(650..1588)
                     /locus_tag="VF516_01060"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX8106277.1"
                     /translation="MTTATTTAWPSLPLEAWLPTRETLHRYTQIIGKIQLALTPMVNH
                     FWNVALRVTSRGLATSALRHDGRTFDIELDLVEHRLIARTDDGAVQARDLRPIAVAEF
                     YREVLAMLGALGIRIAIWDHPVEIPTQPIPFSEDRVHAAYDRDYAARFFRVLTHASEV
                     LEQFRSRFIGKCSGVGFYWGTFDLSVARYSGRRAPEPPSGAIEREAYSHEVSEAGFWP
                     GDRNYAAPAFFALHYPAPDGYTRAAVRPSEARWEAASRSFVLPYEACREQDPHAKILE
                     FCQSTYEAGANLAGWNRAELERSETGGSGPRGIERS"
     gene            1986..>3542
                     /locus_tag="VF516_01065"
     CDS             1986..>3542
                     /locus_tag="VF516_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106278.1"
                     /translation="MDENQRDPAPGMPARLEAPNASSLRKAQVRAALFHKSEPIRIGR
                     FILLEPLGAGAMGEIYAAYDEQLDRKVALKLVRGGSPLTVKADERLLREAQTLAQVSH
                     PNVVQIYEAGTYNGRLFIAMELVRGKTLTSWLGDTAQLPRAVGQREILRQFIAAGRGL
                     EAAHAAGVAHRDFKPDNVLVGDDGRVRVVDFGLARALGEQPEPAEASGEIEGAPSPVI
                     PDDIASGPTVVLEPAAPAEPHPFSTSGATIDLPAPAPAARSGSTGRDPGASAPRLKAA
                     LRLTETGTVMGTPFFMAPEQMRGAIADRRSDQFSFCVALYHALYDSFPFSGRSLNELR
                     DSMQSDEVEFSSGVPVPGFVRRALHRGLSVDPAHRFATMGELLAALSPRVRRHRGWIV
                     AAGLLVIAAAVALVMRLQAPDPCATAGTGMDTEWSPARQVMMHVAFLRSSLPFAEAAW
                     EGVKARLDDYARRWHGDAYAACQATHVEHTQSAAQLDRRMLCLDRGRRQLAALVGELT
                     GGAPDAVEHAV"
BASE COUNT          504 a         1245 c         1305 g          488 t
ORIGIN      
        1 atgctggata ttacaatatc ttgccatcga tcccgtggcc gccaccgctc cgggacgttc
       61 gagctcatcg aatcgctccc gcgagctcgg tgcacggcct ctgcgcggaa gacaagcccg
      121 gtgaatggca ctgcgagctc cgggggcgtc tcatgaagtt gcgtcccgag ctcgcgcgaa
      181 agggcaccag gcgcgcggtg gtcgagcgcg ccggtgcggt cgagctcgtc gcgcgcgctg
      241 cgagctcgat gtacgggccg ccgcagggcg tcgatcggga ggatcagctc cggcatcgcc
      301 tcggtgcgcg gatccgagcg cttcgcgatg ctcgcgggat gacgcaggag atgatcgccg
      361 accgcatcgc ggtcacgccg aagtacgtga gccagctcga gtgcggccag cggtcgccga
      421 gctgggagac cctggtggcg atcgcgcatc atggcttcga gatgcggctc gccgcgctgg
      481 tgttcgggat cgacgaggat atcccggccg aggtgcagga cctcaacgac atgctcgccg
      541 gccggccgaa ggagacgcgc cgcgacctgt tgcaggcgat ggagctgatg ctccgggcgg
      601 gcgcagaacc gaagctgtga gcgcggaccg tcgtggaccg tctggaccgt cagctgcgct
      661 cgatcccacg ggggccgctg ccccccgtct cgctgcgctc gagctcggcc cggttccagc
      721 cggccaggtt cgcgccggcc tcgtacgtgc tctggcagaa ctccaggatc ttcgcgtgcg
      781 ggtcctgctc gcggcacgcc tcgtacggca gcacgaacga gcggctcgcc gcttcccagc
      841 gcgcctcgga cggccgcacc gccgcgcgcg tgtagccgtc gggcgcggga tagtgcagcg
      901 cgaagaacgc cggggcggcg tagttccggt cgcccggcca gaacccggcc tcgctgacct
      961 cgtgcgagta cgcctcgcgc tcgatcgcgc cggacggcgg ctcgggggcg cggcggcccg
     1021 agtagcgcgc gacgctgaga tcgaacgttc cccagtagaa cccgaccccg ctgcacttgc
     1081 cgatgaaccg cgaccggaac tgctccagca cctcggacgc gtgggtcagc acgcgaaaga
     1141 atcgcgcggc gtagtcccga tcgtaggcgg cgtgcacgcg atcctccgag aacgggatcg
     1201 gctgggtcgg gatctccacc gggtggtccc agatcgcgat gcggatgccg agcgcgccga
     1261 gcatcgccag cacctcgcgg tagaactccg ccacggcgat cgggcgcagg tcgcgcgcct
     1321 gcaccgcgcc gtcgtcggtc cgcgcgatca gccggtgctc gaccaggtcg agctcgatgt
     1381 cgaacgtccg gccgtcgtgg cgcagcgccg aggtcgcgag gccgcgcgag gtcacgcgga
     1441 gcgcgacgtt ccagaagtgg ttgaccatcg gcgtcagcgc gagctggatc ttgccgatga
     1501 tctgcgtgta gcgatgaagc gtttcccggg tcggaagcca ggcctcgagc ggaagcgatg
     1561 gccaggcggt cgtcgtggcg gtcgtcatgg tcttgcccga cgaggtgcac gtccgcgacc
     1621 agcccgcgac gcgcgtcgag cccggcggtt cgagtccgga tgggccaccg gcgcacgggg
     1681 catcgcgccg cgccgtgcgg ccatcgctcc ggccggctcg ttcgcggaac acgtcgggcg
     1741 cagcgaaatg cagcacaaaa cagcgctgga ggtcgagcct cggtctggtc tacgctgggc
     1801 cgaactgcga atgcacacat gtgctgacac gcgacggggc ggtcgcggcc gggggacgtc
     1861 gtcgcgcgag aaaaatgccg gccgaggcga tccgtttttc tcgaacccct actacccgga
     1921 cggaagataa cggatcgagc gccgcgaact ccgccgggtg ggcgggcggc gcgaggtcat
     1981 gcaccatgga cgagaaccag cgagaccctg ctccgggcat gcccgcgcgg ctggaagcgc
     2041 cgaacgcctc cagcctccgc aaggcgcagg tccgcgcggc gctgtttcac aagtccgagc
     2101 cgatccggat cgggcggttc atcctgctcg agccgctggg cgcgggcgcg atgggcgaga
     2161 tctacgcggc ctacgacgag cagctcgacc gcaaggtcgc gctcaagctg gtgcgcggcg
     2221 gctcgccgct caccgtcaag gccgacgagc ggctgctgcg cgaggcccag accctggccc
     2281 aggtgtcgca tcccaacgtc gtccagatct acgaggccgg cacctacaac ggccggctgt
     2341 tcatcgccat ggagctggtc cgcggcaaga cgctgaccag ctggctgggc gacacggcgc
     2401 agctgccgcg cgcggtgggc cagcgcgaga tcctgcgcca gttcatcgcc gcgggccgcg
     2461 gcctggaggc cgcccatgcc gccggcgtcg cccaccgcga cttcaagccc gacaacgtcc
     2521 tggtcggcga cgacggccgg gtgcgcgtgg tggacttcgg cctggcgcgc gcgctcggcg
     2581 agcagccgga gccggccgag gcgtcgggag agatcgaggg cgcgccatcg ccggtgattc
     2641 cggacgacat cgcgagcggc ccgacggtgg tcctcgagcc cgccgcgccg gccgagccgc
     2701 atccgttctc gaccagcggg gcgacgatcg atctgcccgc gccggcgccc gccgcgaggt
     2761 ccggatcgac cggccgcgat ccgggggcga gcgcgccccg gctcaaggcc gcgctccggc
     2821 tgaccgagac cggcacggtg atgggcacac cgttcttcat ggcccccgag cagatgcgcg
     2881 gcgcgatcgc cgaccggcgg agcgatcagt tcagcttctg cgtcgcgctc taccacgcgc
     2941 tgtacgacag cttcccgttc tcgggccgga gcctgaacga gctccgcgac tcgatgcaga
     3001 gcgacgaggt cgagttctcg tccggcgtgc cggtgccggg gttcgtgcgc cgcgcgctgc
     3061 accgcggact gtcggtcgac ccggcgcacc gcttcgccac catgggcgag ctgctcgccg
     3121 cgctgtcgcc gcgggtgcgg cggcatcgcg gctggatcgt cgcggcgggg ctgctcgtga
     3181 tcgcggcggc cgtagcgctc gtcatgcgct tgcaggcgcc cgatccgtgc gccacggcgg
     3241 gcaccggcat ggacaccgag tggtcgccgg cgcgccaggt gatgatgcac gtggcgttcc
     3301 tgcgcagcag cttgccgttc gccgaggccg cgtgggaggg cgtcaaggcg cggctcgacg
     3361 actacgcccg gcgctggcac ggcgatgcgt acgccgcgtg ccaggccacg cacgtcgagc
     3421 acacccagtc ggcggcgcag ctcgaccggc ggatgctgtg cctcgatcgc ggccgccgcc
     3481 agctcgcggc gctggtcggc gagctgaccg gcggcgcgcc cgacgcggtc gagcacgccg
     3541 tg
//
LOCUS       DASVOR010000049         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_468223,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000049 DASVOR010000000
VERSION     DASVOR010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468223"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..1925)
                     /locus_tag="VF516_01070"
     CDS             complement(339..1925)
                     /locus_tag="VF516_01070"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HEX8106279.1"
                     /translation="MDTRTVNRAFLLALCGCLAACGTDAPPDPTRVKLADGEVHGSVK
                     GTSLQFLGIPYAKPPVGDLRWKAPQKPDPWSNVRDATQFGKRCAQLMDPTLQNAASND
                     EDCLYLNVWTWSPPPKNPLPVMIWIHGGGNVNGSASEPVPFLNTGVFYSGELLAGGHS
                     VVVVSLNYRLGIFGFFGHPDLWAEGSSGNQGLLDQQMAFQWVHDNIAKFGGDPGNVTI
                     FGESAGSFDVCMHMAAPSNTGLFHRAISESGGCTTVQRTKAQAEAAATSFATGAGCTG
                     AGALACLRGKPAATLMASPDVAAGTGFGPEVDTQFLPDQPRTLYDMGKIAKVPYLLGS
                     NTDEGTLFLPATKPATDPDYMAALTAMFGASASAVANVYMPGNFPSGALNSQTAALAR
                     VIGDSRLVCTTFDTAVRTSHAGASVYMYNFDVPVPALVSTTLGATHGSELTSVFGTSP
                     TFATDPAAKAVSDLMQRYWTNFARTGNPNAGADLSWPAFSESANVRINFALQQPTIVN
                     DFRATECAFWRMGYALQFPM"
BASE COUNT          314 a          753 c          837 g          337 t
ORIGIN      
        1 cgaccgcctg cacgacgccc gcgccgtcgc ggaccacgag gaagctgatc gcgccgagcc
       61 ggcgcacgga ctgcagccat ccgacgacgc gcacgcgctg gccgacgcgc gccgcgacct
      121 ctcccgatcg aacccgttcc atctgacgat ccttgctggc cctgccgtgt ggtgcggaac
      181 gcaaccccgg caggcccagc ggccgctcag gcgcgttcct gcggatcgtc tcccacgatg
      241 gtgatcatcg ctctgtaggc gtacggcacc gcgcgcgagg cgtcaaccgg caacgccggc
      301 gggcgcgcaa gccgcggacc gcgctcggga aaccggcgct acatggggaa ctgcagggcg
      361 tatcccatgc gccagaacgc gcactccgtg gcgcggaagt cgttcacgat cgtcggctgc
      421 tgcagcgcga agttgatccg gacgttggcc gattcgctga acgccggcca gctgaggtcc
      481 gcgccggcgt tggggttgcc ggtcctggcg aagttggtcc agtagcgctg catcagatcg
      541 ctgaccgcct tggcggccgg atcggtggcg aacgtcggcg acgtgccgaa caccgaggtc
      601 agctccgagc cgtgggtggc gccgagcgtg gtcgagacca gcgccgggac cgggacgtcg
      661 aagttgtaca tgtagaccga cgcgccggcg tgggaggtcc gcaccgcggt gtcgaacgtg
      721 gtgcacacca ggcgggagtc gccgatcacc cgggcgagcg cggcggtctg cgagttgagt
      781 gcgccactgg ggaagttccc aggcatgtag acattggcga ccgccgacgc gctggcgccg
      841 aacatcgcgg tcagcgcggc catgtagtcc ggatcggtcg ccggcttggt ggcgggcagg
      901 aacagcgtgc cctcgtcggt gttgctgccg agcaggtacg gaaccttggc gatcttgccc
      961 atgtcgtaca gggtccgcgg ctgatccggc aggaactgcg tgtcgacctc ggggccgaag
     1021 ccagtgccgg cggcgacgtc ggggctcgcc atcagggtcg ccgccggctt gccgcgcaga
     1081 cacgcgagcg cgccggcgcc cgtgcagccg gcgcccgtgg cgaagctcgt ggccgcggcc
     1141 tcggcctggg ccttggtgcg ctgcacggtg gtgcaaccgc cgctctcgct gatcgcgcgg
     1201 tggaacaggc cggtgttgct gggcgccgcc atgtgcatgc agacgtcgaa cgagcccgcc
     1261 gactcgccga agatcgtcac gttgccgggg tcgccgccga acttcgcgat gttgtcgtgc
     1321 acccactgga acgccatctg ctggtcgagc aggccctggt tgcccgagct gccctcggcc
     1381 cacagatcgg ggtgaccgaa gaagccgaag atgccgagcc ggtagttgag gctgaccacg
     1441 acgacgctgt ggccgccggc caggagctcg ccgctgtaga acacgccggt gttgaggaat
     1501 ggcaccggct cggacgccga gccgttgacg ttgccgccgc cgtggatcca gatcatcacc
     1561 ggcaacgggt tcttgggcgg gggagaccac gtccacacgt tgaggtacag gcagtcctcg
     1621 tcgttgctcg cggcgttctg caaggtcggg tccatgagct gggcgcagcg cttgccgaac
     1681 tgggtcgcgt cgcgcacgtt gctccacgga tcgggcttct gcggcgcctt ccaccgcagg
     1741 tcgccgacgg gaggcttggc gtaggggatg ccgaggaact gcaggctggt ccccttcacg
     1801 ctgccgtgga cctcgccgtc cgcgagcttg acccgggtcg ggtcgggcgg ggcgtcggtg
     1861 ccacacgcgg cgaggcagcc gcacagcgcg agcaggaatg cgcggttcac tgtcctggtg
     1921 tccatggcga gctcctggtg gggcgccgcg acggccgtgc gcgcgggcac ggggagcggc
     1981 ggtcggtggc ttcgtggcag ttctgcattg cagaactctg ttccggatcg ggccgaggcc
     2041 ggtgcgactc tcgatcgggc gtcgcaggaa gtcaatcgcg gagccgccgg gcgctgccgc
     2101 gcggtcgctc cgcaggtacg cacgtgggcc gccacgcgcc gacagcgcgc cgcgtcagcg
     2161 cgccaccagc cgggcgcgat cgcggtagtc ggcggcgtag tcgggctggc ccttgtggtc
     2221 ccacgcccgc gcgagctcgc g
//
LOCUS       DASVOR010000050         4114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205160,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000050 DASVOR010000000
VERSION     DASVOR010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4114
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205160"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1391
                     /locus_tag="VF516_01075"
     CDS             84..1391
                     /locus_tag="VF516_01075"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8106280.1"
                     /translation="MTRFAIVWAGQLLSVLGSGVSGFALGVWAYRTTGSVTQFALVLF
                     SAALPGVLLLPVAGLLADRWDRRRTMILSDAGSALGTAALALLFLLDRVEMRWIYVLL
                     TFCSTMGAFQRPAYSAAVAQLVPKEHFGRANGQVRTAQAVSQILAPLLSGALLGPVGI
                     QGILILDLVSFGFALATLLAVRFPPLADDAETGPAASTWAERWRELTAGWSYLVERDS
                     LMALMIFLAGINFSFGLANALVQPLVLSFASPADLGRALTIGGAGLLVGSVVMGTWGG
                     PKRRVHGIFGFVPLAAAGMFLVGLTSSIAWVTAAMVVVFFCLPFIEGCTTAIVQSKVA
                     PRVQGRVFAITHMIAGSMAPISYLLAGPLVDQVFAPLMKPGGPLAGSLGPIFGVGEGR
                     GIALLISVLGIALLLTTLVAFLLPRVRHVEDQLPDMVPDAAGS"
     gene            1430..2536
                     /locus_tag="VF516_01080"
     CDS             1430..2536
                     /locus_tag="VF516_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106281.1"
                     /translation="MRFISILIALTVCTTAHADGDPKLASPPPPEPVREYSTVGIGLV
                     PGLSTDLGRIGSVQHLLSFNLLVGVGGGSSGLSFSSIADIERGPVTGYQVGGVVATAE
                     RIEGTQLAGVAAVAGDAHGLQAAGVAAVAGADTNVQIAGVTGIAGGTADVQIAAALNV
                     AGRVSGLQLASINVARKVDGVQIGAINIGGSTAGVQVGVINIGGSADGFSFGLINIIP
                     GGRYDLETSIDSDKLGTVLFRHGSRRWHNVYGIGAQPVSHSGPNDDVWTYGLGFGHTL
                     PLGATTMDLEAIGWHVNHGPHYETDLSILSQLRLSVAYSLGSLALVAGGNFNVYVTSD
                     SQSPLVLEHRTTGSPMTSGVTVTIWPSAFVGLRI"
     gene            2503..2640
                     /locus_tag="VF516_01085"
     CDS             2503..2640
                     /locus_tag="VF516_01085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106282.1"
                     /translation="MAERVRRPPDLTARYHDDRTHLGLAKPRANVVIRDDKVVLKAKP
                     R"
     gene            3437..>4114
                     /locus_tag="VF516_01090"
     CDS             3437..>4114
                     /locus_tag="VF516_01090"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8106283.1"
                     /translation="MQKGFAYYCGPILARCSKRGGTLAKHQSSALIVIQLGTRDITHN
                     AVAPESWATASVYLFLQNLRKLVGVEAAASAKQDLRVVHVDEKESRKLAQRGDTWRPN
                     SQTGNPDLADLLATLFAHPANAKKINDAREHLDPDAHVFAEPKGRPNRDWARGAMLFG
                     PPGTSKTSLVSAIAASIGWPLIEVHASHFLQEGLDRIPARADWIFARLMELDRCYLGG
                     DSLLGRQE"
BASE COUNT          635 a         1432 c         1302 g          745 t
ORIGIN      
        1 gtgttcgcgc tcgatgcctt ctcgtctgcc gccggcgcgt cgccgtcggc gccttctcca
       61 caatctgcgc gaggtgtcga ccgatgacga gattcgccat cgtctgggcc ggccagctgc
      121 tctccgtcct cggctcgggg gtgtccggct ttgcgctcgg cgtgtgggcc taccggacca
      181 ccggctcggt cacgcagttc gcgctcgtcc tgttctcagc cgctctgccc ggcgtgctgc
      241 tgttgcccgt ggccggcctc ttggccgacc gctgggatcg gcggcggacg atgatcctga
      301 gcgacgccgg ctccgccctc ggaacggcgg cgctggccct gctgttcctg ctcgaccgcg
      361 tcgagatgag atggatctac gtccttttga ccttctgcag cacgatgggc gccttccagc
      421 gcccggccta ctcggcggcc gtcgcgcagc tcgtgccgaa ggagcacttc gggcgcgcca
      481 acggccaggt gcgcacggcg caggcggtgt cgcagatcct ggcgccgctg ctctccggcg
      541 cgctgctcgg cccggtcggc atccagggca tcctgatcct cgacctggtc agcttcggct
      601 tcgccctggc aaccctcctc gccgtccggt ttccgcccct cgccgacgac gccgagaccg
      661 ggccggccgc gtcgacctgg gccgagcgct ggcgagagct caccgccggc tggagctacc
      721 tggtcgagcg cgacagcctg atggcgctga tgatcttcct cgccggcatc aacttctcgt
      781 tcggcctggc caacgcgctg gtgcagccgc tggtgctgtc ctttgcctcg cccgccgacc
      841 tcggcagggc gctcaccatc ggcggcgccg gtctgctcgt gggcagtgtg gtgatgggaa
      901 cctggggcgg accgaagcga cgcgtccacg gcatcttcgg cttcgtgccg ctcgcggcgg
      961 ccggcatgtt cctggtcggc ttgacgtcgt cgatcgcctg ggtcaccgcc gccatggtgg
     1021 tggtcttctt ctgcctgccc ttcatcgagg gctgcaccac cgccatcgtg cagagcaagg
     1081 tggcgccgcg cgtccagggc cgcgtgtttg ccatcactca catgatcgcc ggctcgatgg
     1141 cgccgatcag ctacctcctc gccggccccc tggtcgacca ggtcttcgcg ccgctgatga
     1201 agcccggcgg cccgctcgcc gggtcgctcg gcccgatctt cggcgtcggc gaagggcgag
     1261 gcatcgctct gctcatatcg gtcctcggca tcgccctgtt gctgacgacc ctcgtcgcgt
     1321 tcctcctgcc gagggtgcgc cacgtcgagg accagctgcc cgacatggtg cctgacgccg
     1381 ccggctcatg accgcgtcca tgaatcacgg aacccggagt ccacgagtca tgagattcat
     1441 atcgatcctg atcgcactca ccgtctgtac caccgcgcac gccgacggtg accccaagct
     1501 cgcgtcgccg cctccgcccg agccggtccg cgagtacagc acggtaggca tcggcctggt
     1561 cccggggcta tcgaccgatc tgggccgcat cggctcggtg cagcacctcc tgtcgttcaa
     1621 cctgctggtc ggcgtgggtg gcgggtcgag cgggctgtcg ttcagtagca tcgccgatat
     1681 cgagcgcggg cccgtgactg gctaccaggt cggcggcgtg gtcgcgacgg cggagcgcat
     1741 cgaggggacc cagctggcag gcgtcgcggc ggtggccggc gatgcacatg gcttgcaagc
     1801 ggccggcgtc gccgcggtcg ccggcgccga caccaacgtc cagatcgccg gcgtgaccgg
     1861 catcgcaggt ggcaccgcgg acgtccagat cgcagccgcg ctcaacgtcg caggccgcgt
     1921 ctccggcctt cagctcgcat cgatcaacgt cgcgcgcaag gtcgacggcg tccagatcgg
     1981 cgcgatcaac atcggcggct cgaccgcggg cgtccaggtc ggcgtgatca acatcggcgg
     2041 ctcggccgac ggcttctcgt tcgggctgat caacatcatc ccgggcggcc gctacgatct
     2101 cgagacctcg atcgactcgg acaagctggg cacggtgctg ttccggcacg gcagccggcg
     2161 ctggcacaac gtgtacggta tcggcgccca gccggtgagc cacagcggcc ccaacgacga
     2221 cgtgtggacc tacggcctgg gcttcggcca caccctgccg ctgggcgcca ccacgatgga
     2281 tctcgaggcg atcggctggc acgtcaacca cggcccgcac tacgagaccg acctcagcat
     2341 cctgtcccag ctccggctct cggtcgcgta ctccctgggc tcgctcgcgc tcgtcgccgg
     2401 tggcaacttc aacgtctacg tcaccagcga ttcgcaatcg ccgctggtcc tcgagcaccg
     2461 caccaccggc tcgccgatga ccagcggggt gaccgtcacg atatggccga gcgcgttcgt
     2521 cggcctccgg atctgacggc ccggtaccac gacgaccgca cccacctcgg cctcgccaag
     2581 ccgcgcgcca acgtcgtgat ccgcgacgac aaggtcgtcc tcaaggccaa gccgcgctga
     2641 cggcgcgcgg ctgctgcgtg ctccagcccg cactgacggg ccgcgccatc acctgatgca
     2701 ggcgcgcgcc tgggtgatcg ccggccgtat cccaaacgtt ggcgttcttc cggcttccgg
     2761 atcagccaga ccaattggcc tacctggcta cagccaaact gacttacgcc gtgttcagta
     2821 agttggattg tgcatccgct atcatcccag cgccatcaca ttgaccggga acctggagac
     2881 gtggccgtcg gccaaagcat ctacgaaaat ccggccagaa tatctagtga aaatccaagc
     2941 gtagatgaga agccaggcgc atcataccca ccgaatacct accaaacctt ctgggggctt
     3001 cgcacgctcg aagagtatcg gtcttacgct agtgtgcacg gcgcaccgcc ggtcgacgac
     3061 gaggtgctgc gcaaggctgc tctatcgtgg cagtgggcac ggagcgttct agcgctccaa
     3121 acggcgctat ttgcgacgag cgcgacctcg ttcgacggcg atcaacttgg ttgggcgctt
     3181 gcgattcgtc ttcgttggga cgctggacga ttcgcgggac gagtgacggt agcatcggag
     3241 gaagcgataa tcagcccgcc accagcggag gagcaggagc tgctgcgagc gggacttaat
     3301 gcatacttcc gaactcagct tccgaacggt cgatggcccc gctttggccc gctgttccac
     3361 tacccaaact cggggaatgc gtactgctac ttcttcgaga cccttgcgga gatgctccgc
     3421 ccggccttgc gccgcgatgc agaagggctt cgcctattac tgcggcccca tcttggctcg
     3481 ctgctcaaag cgtggcggta cgctcgcgaa acatcaatcc tcagcgctga tcgtcatcca
     3541 acttggaact cgggacatca cccacaacgc cgtagcgccg gaatcatggg ctactgcgtc
     3601 cgtataccta ttcctccaga acctccgaaa gctcgtcggt gtcgaggcgg ctgcaagcgc
     3661 gaagcaggat ctgcgagtcg tgcacgtcga tgagaaggag tcacgcaagc ttgcgcaacg
     3721 tggcgacacg tggcggccta actcgcaaac tggaaatccg gatcttgcgg atctcctcgc
     3781 tacgctcttt gcgcatccgg cgaatgcgaa gaagatcaac gacgcacgtg agcacctgga
     3841 tcctgatgcc cacgtattcg cggagcccaa ggggcgaccc aaccgcgact gggcacgtgg
     3901 ggcgatgctt ttcggcccac cgggcacgtc gaagacgtcg cttgtgagcg cgatcgcagc
     3961 ctcgattggt tggccattga tcgaagtcca cgcgtcgcac ttccttcagg agggccttga
     4021 tcgaattccg gcgcgtgccg actggatctt tgcgcgcttg atggaactcg atcgctgtta
     4081 tcttggtgga gactccctcc ttggccgtca agaa
//
LOCUS       DASVOR010000051         3437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_526634,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000051 DASVOR010000000
VERSION     DASVOR010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526634"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VF516_01095"
     CDS             complement(<1..482)
                     /locus_tag="VF516_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8106284.1"
                     /translation="MQIAEPGESRVKEACKPRVVGIDLGTTNSLVAHIADASPQVLGS
                     DPLVPSVVHYAADGSVVVGRAAQAHALDAPHDTLASVKRLIGRGARDLGPVRAMLPYE
                     LDGDDRAVRIRLSTGRLVSPVEVSAEILRELKRRAEVVLGGPVEGAVITVPAYFDDAQ
                     R"
     gene            complement(516..1073)
                     /gene="hscB"
                     /locus_tag="VF516_01100"
     CDS             complement(516..1073)
                     /gene="hscB"
                     /locus_tag="VF516_01100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEX8106285.1"
                     /translation="MANPSSSERGTAAASSTGGGDDPFALLGLPAAFDVDPAALERAF
                     FERSKAVHPDRVAGASAAERVAALSRSRALNDAYQLIKKPVSRAEYLLARAGVTIGDN
                     ERLEPAFLMEILELREELAEARTAGELARVEQLQRAMQARRAEALDALPGLFAAGDLA
                     AIKHRLIVLRYIHRYLEECDAALDE"
     gene            complement(1051..1482)
                     /locus_tag="VF516_01105"
     CDS             complement(1051..1482)
                     /locus_tag="VF516_01105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEX8106286.1"
                     /translation="MSATTQPLPAPPTALTASTAASTASTPAPAAAPSRREIEITAAA
                     AAEIARQREKRGTSDAMIRIGVRGGGCTGFTYVFEWAEQVRATDRVFSAHGVSIVVDP
                     KSLVYLGGMQLDFVRGMMGHGFKFNNPNAKGSCGCGESVQF"
     gene            complement(1501..1941)
                     /gene="iscU"
                     /locus_tag="VF516_01110"
     CDS             complement(1501..1941)
                     /gene="iscU"
                     /locus_tag="VF516_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEX8106287.1"
                     /translation="MAYSDKVIDHYENPRNVGTFKAQSDVDASEVGTGLVGAPACGDV
                     MRLEIRVKDGLIADAKFKTFGCGSAIASSSLATEMIKGMTLDQAAQIKNTQIVEELNL
                     PPVKIHCSVLAEDAIKAAIEDYKKKQAPRAAGSASAVAAKPQDA"
     gene            complement(1961..3244)
                     /locus_tag="VF516_01115"
     CDS             complement(1961..3244)
                     /locus_tag="VF516_01115"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEX8106288.1"
                     /translation="MVAEATHTPASPTSIAIAVPDGVKLPIFMDNHSTTAVDLRVVQA
                     MLPFFTQHYGNAASRNHSFGWTAEAAVDHARAQVAALLGGDAKEIVWTSGATEANNLA
                     IKGAASYLRRQGNHIITVETEHKSVLDTCKRLQREGFEVTYLTPGKDGLVTPEQVTSA
                     MTDKTILVTIMLANNEIGVVQPIEAIGAAVKAKNPRTLFHTDAVQGFGKVPFDADKAR
                     VDLASVTAHKLYGPKGIGALWVRRKPRVRIDPIIDGGGHERGMRSGTLPVPLIVGFGK
                     AAELAREEMAGEAAQLTALRDRLYKGLTSRLTETYVNGSLEHRLPGNLNISFAFVEGE
                     SLLMALKDVAVSSGSACTSASLEPSYVLRALGVGDELAHTSIRFGIGRFNTEAEIDYV
                     VELVVSSVERLRQLSPLWEMHQEGIDLNSIQWTPH"
     gene            complement(3245..>3437)
                     /locus_tag="VF516_01120"
     CDS             complement(3245..>3437)
                     /locus_tag="VF516_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106289.1"
                     /translation="TACGVCDHHGHQKGEAPRLFAGLSRQPAHAAAGPASPASTDDTR
                     DKPRDFDVPVTVLYREGNN"
BASE COUNT          458 a         1224 c         1195 g          560 t
ORIGIN      
        1 cgctgggcgt cgtcgaagta cgccggcacc gtgatcaccg cgccctcgac cggtccgccg
       61 agcaccacct cggcgcgccg cttgagctcg cgcaggatct cggcggacac ctccaccggc
      121 gacaccagcc gcccggtcga cagccggatc cgcacggcgc ggtcatcgcc gtcgagctcg
      181 tagggcagca tcgcgcgcac cgggccgagg tccctggcgc cgcggccgat cagccgcttg
      241 accgaggcca gcgtgtcgtg cggcgcatcg agcgcgtggg cctgcgccgc ccggccgacc
      301 acgacgctgc cgtcggctgc gtagtgcacc accgacggga cgagcggatc gctgccgagc
      361 acctggggcg acgcgtcggc gatgtgggcg accaggctgt tcgtggtccc gaggtcgatc
      421 ccgaccaccc gcggcttgca cgcctccttg accctggact cccccggctc tgcgatttgc
      481 atcaacatcg gttgtctcga ttcttgcccg cccggctact catcgagcgc ggcgtcgcac
      541 tcttcgaggt agcggtggat atagcgcagc acgatcagcc ggtgcttgat cgccgcgaga
      601 tcgccggcgg cgaacagccc gggcagcgcg tcgagcgcct cggcccggcg cgcctgcatc
      661 gcgcgctgca gctgctcgac ccgggcgagc tcgcccgcgg tgcgggcctc ggcgagctcc
      721 tcgcgcagct ccaggatctc catcaggaac gccggctcca gccgctcgtt gtcgccgatc
      781 gtgaccccgg cgcgcgcgag caggtactcg gcgcgcgaca ccggcttctt gatcagctgg
      841 taggcatcgt tgagcgcgcg cgaccgcgac agcgccgcga cccgctcggc cgccgacgcc
      901 ccggcgaccc ggtcggggtg caccgccttg ctgcgctcga agaacgcgcg ctccagcgcc
      961 gccggatcga cgtcgaacgc ggccggcagc ccgagcagcg cgaacggatc gtcgccgcct
     1021 ccggtggagg atgccgcggc ggtgccgcgc tcagaactgg acggattcgc cacagccgca
     1081 cgagcccttg gcgttgggat tgttgaactt gaagccgtgg cccatcatcc cgcgcacgaa
     1141 gtcgagctgc atgccgccga ggtagaccag gctcttggga tcgacgacga tcgacacccc
     1201 gtgcgccgag aacacccggt cggtcgcgcg gacctgctcg gcccactcga acacgtaggt
     1261 gaagccggtg cagccgccgc cgcgcacacc gatgcggatc atcgcgtcgg acgtgccgcg
     1321 cttctcgcgc tgccgggcga tctccgccgc cgccgccgcg gtgatctcga tctccctcct
     1381 ggacggcgcg gccgccgggg ccggcgtcga cgccgtcgaa gctgcggtcg aggccgtcaa
     1441 cgcggtcggc ggcgcgggga ggggctgggt ggtcgcggac atcttctctc ctgggccgtc
     1501 ctacgcgtcc tggggcttcg cggcgacggc gctggcgctg ccggcggccc gcggcgcctg
     1561 cttcttcttg tagtcctcga tcgcggcctt gatcgcgtcc tcggccagca ccgagcagtg
     1621 gatcttgacc ggcgggaggt tgagctcctc gacgatctgg gtgttcttga tctgcgccgc
     1681 ctgatcgagc gtcatgccct tgatcatctc ggtcgcgagc gacgacgagg cgatcgccga
     1741 gccgcagccg aacgtcttga acttggcgtc ggcgatcagg ccgtccttga cgcggatctc
     1801 gagccgcatc acgtcgccgc acgccggggc gccgaccagg ccggtcccca cctcgctggc
     1861 atcgacgtcg gactgcgcct tgaacgtgcc cacgttccgg gggttctcgt agtgatctat
     1921 gaccttgtct gagtacgcca tgatggttct tcttgatagc tcagtgaggg gtccactgga
     1981 tggagttgag atcgatgcct tcctggtgca tctcccacag cggcgacagc tggcgcagcc
     2041 gctcgacgga ggacaccacc agctcgacga cgtagtcgat ctcggcctcg gtgttgaacc
     2101 ggccgatgcc gaaccggatc gacgtgtggg cgagctcgtc gccgacgccg agcgcgcgca
     2161 gcacgtagct cggctcgagc gacgccgagg tgcacgccga gcccgacgac accgcgacgt
     2221 ccttgagcgc catcagcagc gactcgccct cgacgaacgc gaacgagatg ttgaggttgc
     2281 cgggcagccg gtgctcgagc gagccgttga cgtaggtctc ggtcagccgc gaggtcagcc
     2341 ccttgtacag ccggtcgcgc agcgcggtca gctgcgcggc ctcgccggcc atctcctcgc
     2401 gggcgagctc ggccgccttg ccgaacccga cgatcagcgg caccggcagc gtgcccgatc
     2461 gcatgccgcg ctcgtggccg ccgccgtcga tgatcgggtc gatccgcacc cggggcttgc
     2521 ggcgcaccca cagcgcgccg atgcccttgg ggccgtacag cttgtgcgcg gtgaccgacg
     2581 cgaggtcgac ccgggccttg tcggcgtcga acggcacctt gccgaagccc tgcacggcgt
     2641 cggtgtggaa cagcgtcctg gggttcttgg ccttgaccgc cgcgccgatc gcctcgatcg
     2701 gctggaccac gccgatctcg ttgttcgcca gcatgatcgt gacgaggatc gtcttgtcgg
     2761 tcatcgcgga cgtcacctgc tcgggggtga ccaggccgtc cttgcccggc gtcaggtagg
     2821 tgacctcgaa gccctcgcgc tgcagccgct tgcaggtgtc gagcacgctc ttgtgctcgg
     2881 tctccaccgt gatgatgtgg ttgccctgcc ggcgcaggta cgacgcggcg cccttgatcg
     2941 cgaggttgtt ggcctcggtc gcccccgacg tccagacgat ctccttggcg tcgccgccga
     3001 gcagcgccgc gacctgcgcg cgcgcgtggt cgaccgcagc ctcggcggtc cagccgaacg
     3061 agtggttgcg gctggcggcg ttgccatagt gctgcgtgaa gaacggcagc atcgcctgga
     3121 cgacccgcag atcgaccgcg gtcgtcgagt ggttgtccat gaagatcggc agcttcacgc
     3181 cgtcggggac cgcgatcgcg atcgacgtcg gcgatgccgg cgtatgtgtt gcctctgcga
     3241 ccatttagtt attgccctct cggtaaagca cggtgaccgg gacgtcgaag tcccggggct
     3301 tgtcacgggt gtcgtcggtg ctggccgggc tggccgggcc ggccgcggcg tgcgccggct
     3361 ggcgggacag cccggcgaac agccgcggtg cctcgccctt ctggtggccg tggtgatcgc
     3421 acacgccgca tgcggtc
//
LOCUS       DASVOR010000052         7836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_351298,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000052 DASVOR010000000
VERSION     DASVOR010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351298"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="VF516_01125"
     CDS             <1..486
                     /locus_tag="VF516_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8106290.1"
                     /translation="NAALNPAYGSMSQIPDDAFDKILGANIRSNHWLAQMVLPDMIAK
                     RDGAIIIVSSIGGYRGSTTIGTYCISKAADLQLARNLAVENGPHNIRVNCIAPGLVKT
                     DFARALWDTEEGERRASSGTPLRRLGEPDDIAGAAVFLASRAGAWMTGQSIVVDGGVL
                     A"
     gene            complement(503..799)
                     /locus_tag="VF516_01130"
     CDS             complement(503..799)
                     /locus_tag="VF516_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HEX8106291.1"
                     /translation="MVRHILLLQQRPDATADEIDACHAGLASLVGPIPGLINFHWGAN
                     FAPAERRDGFTHGFSMDFADRASLAAYGPHPQHQVAAARVRATFERVVVFDFEL"
     gene            892..1305
                     /locus_tag="VF516_01135"
     CDS             892..1305
                     /locus_tag="VF516_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8106292.1"
                     /translation="MTIAIDRFDHIVLNVRDVEASAAWYAEVLGMQREDFASRTGTRV
                     AMRFGRQKINLRPVDTDAEAWFTGAHPTAGSDDLCFITPHTPAQVVAHLQARRVPIEA
                     GPVERAGALGPMTSVYCRDPDGNLIEIAWYPPAGG"
     gene            complement(1331..2482)
                     /locus_tag="VF516_01140"
     CDS             complement(1331..2482)
                     /locus_tag="VF516_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106293.1"
                     /translation="MIGSVAVCAAACSVRESSVALALSAVGCADGQREGFVDLAMYPD
                     IAGCSGAWTIPGIHTEAPGTAPACPGLATTDTTVPACGRNAGDDSANPLGMGCNVADL
                     CAVGWHVCTGADDLVRSSPTGCAGVTGSDDGPLFFATRQSSTGCGQCATGTRTDAACD
                     SASCTPGCLQTAATSNDFFGCGNFGSSPPVDACGPLDRFSQNLCSGLPGSPWSCDLAT
                     TADDSGLCEAYTVTKADISHGGVVCCRDPDQPPDCSGAAADPAALWPPNHALVSIHIT
                     GVVDPDPGDTVTISITSIFQDEPVNTTGDGNTDVDGTGVGTSTAQVRAERTGDPHVPG
                     DGRVYHIDFVATDRAGQTCTGSVTVCVPHDQGQGSTCADGGPLFDSTAP"
     gene            complement(2657..2935)
                     /locus_tag="VF516_01145"
     CDS             complement(2657..2935)
                     /locus_tag="VF516_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106294.1"
                     /translation="MIARFLLLLAAASAACGSSNDAGCHSNEVEVDNFRGARDGAVVC
                     KPIPASCNGTASCADNSCIRDMYGLCDAPYLGVACSDTFPPTIISCNP"
     gene            3021..3710
                     /locus_tag="VF516_01150"
     CDS             3021..3710
                     /locus_tag="VF516_01150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8106295.1"
                     /translation="MRILLVRHGESLGNVDPRVHATTADHAVPLSERGNEQAREAGVR
                     IADYLDQHVQGERPHIRLWVSPYHRTRQTADGLVETARGWITDRVEHILLCEQQFGLF
                     DGVAEGELPQRFPNEWAYYDMQCRFGGKFWARMPQGESRFDVAKRIHQAFGTFHRDAL
                     EHGIHDIVVICHGVTLRAFVMMWCHLSPEWFEAEPNPNNCAIRLIEDGTDRGYVFPGF
                     PRAGRAGGSGR"
     gene            complement(3717..5684)
                     /locus_tag="VF516_01155"
     CDS             complement(3717..5684)
                     /locus_tag="VF516_01155"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX8106296.1"
                     /translation="MPSLASARVTRIEITQIESPAFGGATFGATGAYEKLVGRAFGEV
                     DPRAPESREITDIELGPRNARGMVEYDTGIIILRPIDPTRGNHRLFYEVTNRGVILSL
                     AALNASPNPSTTNPTAADAGNGFLMREGFTILFTGWDLSAIGTFTSHYPVATQRNGSP
                     IVGPAVEELIFDSPPASQPTTATLTYPAAALDTAQATLTVRAHYGDAQTAIPPTGWRF
                     NDARTIQLLPEGTRFATGALYELEYQARDPVVAGLALAAVRDIPAFLHHATADDTGAP
                     NPLAGRIERIIAHGISQPARLLHDFVRLGFNRDERGRPVFDGILDYIAGASSGFFNYR
                     FAQPFRTHRQHIGRWTPERVFPFANQLVFDPVTEQFGGRLIRCTLTGTCPKILEANSA
                     NEYWVKGGSLLHTDTRGHDLRFDPPGVRSYFFSSFPHSPAAGAGICEQSRNPLNAGPG
                     LRALLIDLDDWISHRAEPPASRLPRRFNHTLVPSLPQSGVGFPAIPGVRYTGTMSTGD
                     LFDFGRRFDDGVLDFAARADAIVSPVVASPYPVFVPATDADGNDAAGVRFPDVAVPLA
                     TYTGYGFRAGPSSADLCDAFGQMIPFAKTRADRAAAGDPRPSLEERYSSHQAYVDQVR
                     AAAEALAAERFLLAEDVDRFVQAAQASSVGN"
     gene            complement(6032..7075)
                     /locus_tag="VF516_01160"
     CDS             complement(6032..7075)
                     /locus_tag="VF516_01160"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HEX8106297.1"
                     /translation="MTDLAPSLTPSLAPSLCTSWLGLSLRSPLVIAAGPLTHDVEAVA
                     AAVGAGAGAVIMHSLFEEQLIAEQLAAHRFIDARIDHDAEAASFLPERAVFPLGAEPY
                     LAQLDRLRRRVDVPILASLNGTTPGGWTDHARQLEAGGASAIELNLYDVATSPDESGA
                     EVEDRQLAVVRAVTAAVRIPVTIKLSPFYTSIPAFLRRLEAAGARGAALFNRFYQPDI
                     DLETLDVDKKLVFSTPRELPLRLHALAICSPDTPLALACTGGVHTGSDVAKAIVCGAD
                     VVQIASALLERGPDYLTVIRDQLVRWLRDKDYASSDEARGVLARNSAPDRHAWERLQY
                     TRMIEGWKPRRHA"
     gene            complement(7072..>7836)
                     /locus_tag="VF516_01165"
     CDS             complement(7072..>7836)
                     /locus_tag="VF516_01165"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8106298.1"
                     /translation="QRDRLVELARALDRDGSPDARALAGLAHHLVPRSVWILGGDGWA
                     YDIGYGGLDHVLASHRKVNVLVLDTEVYSNTGGQQSKATPLGAAAKFAAAGKASRKKD
                     LGLLAMSYGHVYVASVALHTKSAQTVQALLEAEAHPGPSLVIAHSPCIAHGYDLVHAP
                     AQQKRAVDSGMWPLYRFDPARAAHGEPALALDCRAPHLDVAQYMENETRFRMVELRSP
                     ERYQALVGAARDAVRQRWSMYEQLAKIHLPASEDAQ"
BASE COUNT         1109 a         2551 c         3065 g         1111 t
ORIGIN      
        1 aacgcggcct tgaaccccgc gtatggctcg atgtcgcaga tcccggacga tgcgttcgac
       61 aagatcctcg gggccaacat ccggtcgaat cactggctcg cgcagatggt gcttcccgac
      121 atgatcgcga agcgcgacgg cgcgatcatc atcgtgtcct cgatcggcgg ctatcgcggt
      181 tcgacgacga tcgggacgta ctgcatctcg aaggcggcgg acctccagct cgcgcgcaac
      241 ctggcggtcg agaacggacc gcacaacatc cgcgtcaact gcatcgcgcc ggggctggtc
      301 aagaccgact tcgcccgggc gctgtgggac acggaggagg gggagcgccg cgcgtcgtcc
      361 ggcacgccgc tccggcggct cggcgagccc gacgatatcg cgggtgcagc cgtgttcctg
      421 gccagccgcg ccggggcgtg gatgaccgga cagtcgatcg tcgtcgacgg cggcgtgctg
      481 gcctgaggcc cgcgcgcgcc ggtcacagct cgaagtcgaa caccacgacg cgctcgaacg
      541 tcgcgcggac ccgggcggcg gcgacctggt gctggggatg cgggccgtag gcggcgaggc
      601 tcgcgcggtc ggcgaagtcc atgctgaagc cgtgggtgaa gccgtcgcgg cgctcggcgg
      661 gagcgaagtt ggcgccccag tggaagttga tgaggcccgg gatcgggccg accaggctcg
      721 cgagcccggc gtggcacgcg tcgatctcgt cggcggtggc gtcggggcgc tgttgcagca
      781 ggaggatatg gcgcaccatc gcgggcgagc ctagcgaaac cgggcggcga tggcgatcac
      841 cgcgcgcggc tgcgtgtaca gtcgcgacgc tcccggcgcc ggatccgcaa gatgacgatc
      901 gcgatcgacc gcttcgacca catcgtgctg aacgtccggg acgtcgaggc cagcgcggcc
      961 tggtacgccg aggtgctggg catgcagcgc gaggacttcg cgtcgcgcac cggcacgcgc
     1021 gtggcgatgc ggttcggccg gcagaagatc aacctccgcc cggtcgatac cgacgcggag
     1081 gcctggttca ccggcgctca cccgaccgcg ggcagcgacg atctgtgctt catcaccccg
     1141 cacacgcccg cgcaggtggt cgcgcatctc caggcccgcc gggtgcccat cgaggccggg
     1201 ccggtcgagc gcgccggtgc gctcgggccg atgacgtcgg tctactgccg cgaccccgac
     1261 ggcaacctga tcgagatcgc gtggtatccg ccggcgggcg gctgatcgcc acgcgtgagc
     1321 gggtcgcgga tcagggagcg gtcgaatcga acagcggccc gccgtcggcg caggtgctgc
     1381 cctggccctg atcgtgcgga acacagacgg tgacgctccc ggtgcaggtc tggccggcgc
     1441 ggtcggtggc gacgaagtcg atgtgataga cgcgaccgtc gccgggcacg tgcggatcgc
     1501 ccgtgcgctc ggcgcggacc tgcgcggtgc tggtcccgac gccggtgcca tcgacatcgg
     1561 tgttgccatc gccggtggta ttgaccggct cgtcctggaa gatcgaggtg atcgagatcg
     1621 tcacggtgtc accggggtcg gggtcgacca cgccggtgat gtggatcgac accagtgcgt
     1681 ggttcggcgg ccagagcgcg gcgggatcgg cggccgcgcc cgagcagtcc ggcggctgat
     1741 cgggatctcg gcagcacacg accccgccgt gcgagatatc ggccttggtg acggtgtagg
     1801 cctcgcagag gccgctgtcg tcggcggtcg tggcgagatc gcatgaccac ggcgagccgg
     1861 ggaggccgct gcacaggttc tgggagaaac gatcgagtgg gccgcaggcg tcaaccggcg
     1921 gagacgagcc gaagttaccg cagccgaaga agtcgttgga cgttgcggcg gtctgcaggc
     1981 agccgggcgt gcacgacgcg gagtcgcatg ccgcgtcggt gcgcgtgccg gtcgcgcact
     2041 ggccgcagcc ggtgctgctc tggcgcgtcg cgaagaacag cgggccgtcg tccgatccgg
     2101 tcacgccggc gcagccggtc ggtgacgacc gcacgaggtc gtcggcgccg gtgcagacgt
     2161 gccagcccac ggcacagaga tcggcgacgt tgcaccccat gccgagcggg ttcgcgctgt
     2221 cgtcgccggc gttgcgaccg caggccggga cggtggtgtc ggtggtcgcg aggccgggac
     2281 acgccggcgc cgtgcccggg gcctcggtgt gaatgccggg gatcgtccac gcgccgctgc
     2341 agcccgcgat gtcgggatac atcgccaggt cgacgaagcc ttcgcgctgg ccgtcggcgc
     2401 accccaccgc gctgagcgcg agtgcgacgg aggactcgcg tacactgcac gcggcggcgc
     2461 acaccgcaac ggaaccgatc aacagtctgg caatgacatg cctcattggg cctccctcgg
     2521 gcgtcggtct gctggtggga gggcgcgtgg gatcggtcgc gcgttttctc gcggcgcacg
     2581 agcagtgtcg gtgctgtgtt gaccggagac ctacagcgtc attggcaaga tgtggcacgt
     2641 caccggatat gggtcatcac ggattgcagc tgatgatcgt cggcgggaag gtgtccgagc
     2701 aggcgacgcc gaggtacggg gcgtcgcaca ggccgtacat gtcacggatg cagctgttgt
     2761 cggcgcacga ggcggtgccg ttgcagctcg ccgggatcgg cttgcacacg acggcgccgt
     2821 cgcgcgcgcc gcggaagttg tcgacctcga cctcgttgct gtggcatccg gcgtcgttcg
     2881 acgagccgca cgccgcgctg gcggctgcga ggaggagcag gaaccgggcg atcatcgggg
     2941 cgagtgcagc gcaccgccgc gaccgggtca attcgccacg gacggtttcg tgccggccgg
     3001 agtcggcact aggatacggt gtgcgcatcc tgctggttcg ccacggtgag tcgctcggca
     3061 acgtcgaccc gcgcgtgcac gcgacgaccg ccgaccacgc ggtaccgctg tcggagcgcg
     3121 ggaacgagca ggcgcgcgag gccggcgtgc ggatcgcgga ctacctcgat cagcacgtgc
     3181 agggcgagcg gccgcacatc cggctgtggg tcagcccgta ccaccggaca cggcagaccg
     3241 ccgacgggct ggtcgagacc gcgcgcggct ggatcaccga tcgcgtcgag cacatcctct
     3301 tgtgcgagca gcagttcggg ctgttcgacg gcgtggccga gggcgagctg ccgcagcggt
     3361 tcccgaacga gtgggcgtat tacgacatgc agtgccggtt cggcggcaag ttctgggcgc
     3421 gcatgccgca gggcgagagc cggttcgacg tcgccaagcg gatccaccag gcgttcggca
     3481 cgttccaccg cgacgcgctc gagcacggga tccacgacat cgtcgtgatc tgccacggcg
     3541 tgacgctgcg cgcgttcgtg atgatgtggt gccacctgtc gccggagtgg ttcgaggccg
     3601 agccgaaccc gaacaactgc gcgatccggc tgatcgagga tggcaccgat cggggctacg
     3661 tgttccccgg gtttccacgc gccgggcgcg ccgggggctc cgggcgctag gctaggtcag
     3721 ttgccgacgc tgctggcctg ggcggcctgg acgaagcgat cgacgtcctc ggcgagcagg
     3781 aagcgctcgg ccgcgagcgc ctcggcggcg gcgcggacct gatcgacgta ggcctggtgg
     3841 ctggagtagc gctcctcgag cgagggccgc ggatcgccgg cggcggcgcg gtcggcgcgg
     3901 gtcttcgcga acgggatcat ctggccgaac gcgtcgcaca ggtcggcgct gctcggcccg
     3961 gcgcggaagc cgtagccggt gtaggtggcg agcgggaccg cgacgtccgg gaaccggaca
     4021 ccggcggcgt cgttgccgtc ggcgtcggtc gccggcacga acaccgggta cggcgaggcg
     4081 acgaccggcg agacgatcgc gtcggcgcgg gctgcgaagt cgaggacgcc gtcgtcgaac
     4141 cgccggccga agtcgaacaa gtcgccggtc gacatcgtgc cggtgtagcg cacgccgggg
     4201 atcgcgggga acccgacgcc gctctgcggc aaggacggca ccagcgtgtg gttgaaccgg
     4261 cggggcagcc ggctcgccgg cggctcggcg cggtggctga tccagtcgtc gaggtcgatc
     4321 agcagcgcgc gcaggccggg gcccgcattc agcgggttgc ggctctgctc gcagatcccg
     4381 gcgccggcgg cgggcgagtg cgggaagctg gagaagaagt agctgcgcac gccgggtgga
     4441 tcgaaccgca gatcatggcc gcgggtgtcg gtgtgcagca gcgagccgcc cttgacccag
     4501 tactcgttgg cggagttggc ctcgaggatc ttgggacagg tgccggtcag cgtgcagcgg
     4561 atcagccggc cgccgaactg ctcggtgacc ggatcgaaca ccagctggtt ggcgaacggg
     4621 aacacccgct cgggagtcca ccgcccgatg tgctggcggt gggtgcggaa cggctgggca
     4681 aagcgatagt tgaagaatcc gctgctcgcg cccgcgatgt agtcgaggat gccgtcgaac
     4741 accgggcggc cgcgctcgtc gcggttgaac ccgagccgga cgaagtcgtg gagcagccgc
     4801 gccggctgcg agatgccgtg cgcgatgatc cgctcgatcc ggccggcgag cgggttgggg
     4861 gcgccggtgt cgtcggcggt cgcgtggtgg aggaacgccg ggatgtcgcg cacggcggcc
     4921 agcgcgaggc cggcgaccac cggatcgcgc gcctggtact cgagctcgta gagcgcgccg
     4981 gtggcgaacc gcgtgccctc gggcaacagc tggatcgtgc gcgcgtcgtt gaaccgccag
     5041 ccggtcggcg ggatcgcggt ctgggcatcg ccgtagtggg cgcgcacggt gagcgtggcc
     5101 tgcgcggtgt cgagcgccgc ggccgggtag gtcagcgtgg cggtggtcgg ctggctcgcc
     5161 ggcgggctgt cgaagatcag ctcctcgacg gcggggccga cgatcggcga gccgttgcgc
     5221 tgggtggcga ccgggtagtg cgaggtgaac gtgccgatcg cggacaggtc ccagccggtg
     5281 aacaggatcg tgaagccctc gcgcatcagg aagccgttgc cggcgtcggc cgcggtcggg
     5341 ttggtcgtcg acgggttggg gctggcgttg agcgccgcca gcgacaggat cacgccgcgg
     5401 ttggtgacct cgtagaacag ccggtggttg cccctggtcg gatcgatcgg gcgcaaaatg
     5461 atgatgccgg tgtcgtactc gaccatgccg cgggcgttgc gcggcccgag ctcgatgtcg
     5521 gtgatctcgc ggctctcggg cgcgcgcgga tcgacctcgc cgaacgcgcg cccgaccagc
     5581 ttctcgtagg cgccggtggc gccgaacgtg gcaccgccga acgccggcga ctcgatctgg
     5641 gtgatctcga tccgcgtgac gcgggccgag gcgaggctcg gcacgcacag acaaccgatc
     5701 gcagccgcga gccggatcct cgccgcggcg cgtggggaaa cttcccgtac ttccatgaca
     5761 gctctcctcg ttggggcgac aggcgcgggt ccgatcctcg tttgtcgcgc cgcagcatgt
     5821 cgagagccga tctccactcc cgccgggtgg tgcgccggag cgcgatcgag gaatcgcgtg
     5881 gttccccgat cgggatcgcc gatctcgcgg agatctgacg atgatcgtcg tcgggggcgc
     5941 ccggtgagcc cggccccact ggcaccccgg aaatggaatg gtcggatcga acgaggaacc
     6001 ggggcggatc accgcaccgg cgcgccggcg gtcacgcgtg gcgccgcggc ttccagcctt
     6061 cgatcatgcg ggtgtactgc aggcgctccc aggcgtggcg atcgggcgcc gagttgcgcg
     6121 ccagcacgcc gcgcgcctcg tcgctcgacg cgtagtcctt gtcgcgcagc cagcgcacga
     6181 gctggtcgcg gatcacggtg aggtagtccg ggccgcgctc gagcagcgcc gaggcgatct
     6241 ggaccacgtc ggcgccgcac acgatcgcct tggcgacgtc ggagccggtg tgcacgccgc
     6301 cggtgcaggc cagcgccagc ggggtgtcgg gcgagcagat cgcgagcgcg tggagccgca
     6361 gcggcagctc gcgcggggtc gagaacacca gcttcttgtc gacgtcgagg gtctcgaggt
     6421 cgatgtcggg ctggtagaac cggttgaaca gcgccgcgcc gcgcgcgccg gcggcctcga
     6481 gccggcgcag gaacgccggg atcgaggtgt agaacggcga cagcttgatg gtgaccggga
     6541 tgcggaccgc ggcggtgacc gcgcggacca ccgcgagctg gcggtcctcg acctcggcgc
     6601 cggactcgtc gggcgaggtc gcgacgtcgt agaggttgag ctcgatcgcg ctcgcgccgc
     6661 cggcctcgag ctggcgcgcg tggtcggtcc agccgccggg cgtcgtgccg ttgagcgacg
     6721 cgaggatcgg cacgtcgacc cgccggcgca gccggtcgag ctgggcgagg tagggctcgg
     6781 cgccgagcgg gaacaccgcg cgctcgggga ggaagctcgc ggcctcggcg tcgtggtcga
     6841 tccgcgcgtc gatgaagcgg tgcgcggcga gctgctcggc gatcagctgc tcctcgaaca
     6901 gcgagtgcat gatcaccgcg ccggcgccgg ccccgaccgc cgcggcgacg gcctcgacgt
     6961 cgtgggtcag cgggccggcg gcgatcacca gcgggctgcg caggctcagc ccgagccagg
     7021 atgtgcagag ggatggcgcg agggagggag ttagcgacgg ggcgaggtca gtcattgggc
     7081 gtcctccgac gcgggcaggt ggatcttggc gagctgctcg tacatcgacc agcgctggcg
     7141 cacggcgtcg cgcgcggcgc cgaccagcgc ctggtagcgc tcgggcgagc gcagctcgac
     7201 catgcggaac cgggtctcgt tctccatgta ctgggcgacg tcgaggtgcg gcgcgcggca
     7261 gtcgagcgcc agcgcgggct cgccgtgcgc ggcgcgcgcc gggtcgaagc ggtacagcgg
     7321 ccacatcccg ctgtcgaccg cgcgcttctg ctgcgccggc gcgtggacca ggtcgtagcc
     7381 gtgggcgatg cacgggctgt gcgcgatgac cagcgacggc ccggggtggg cctcggcctc
     7441 gagcagcgcc tggacggtct gcgcgctctt ggtgtgcagc gcgacgctgg cgacgtagac
     7501 gtggccgtag ctcatcgcga gcaggccgag gtccttcttg cggctcgcct tgccggcggc
     7561 ggcgaacttg gcggccgcgc cgagcggcgt cgccttggac tgctggccgc cggtgttgga
     7621 gtagacctcg gtgtcgagca cgaggacgtt gaccttgcgg tgcgacgcca ggacgtggtc
     7681 gagcccgccg tagccgatgt cgtaggccca gccgtcgccg ccgaggatcc acacgctgcg
     7741 cggcacgagg tggtgggcga ggccggcgag cgcgcgggcg tcgggcgagc cgtcgcggtc
     7801 gagcgcgcgg gcgagctcga cgagccggtc gcgctg
//
LOCUS       DASVOR010000053         4258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_175900,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000053 DASVOR010000000
VERSION     DASVOR010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4258
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175900"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..851
                     /gene="hisF"
                     /locus_tag="VF516_01170"
     CDS             84..851
                     /gene="hisF"
                     /locus_tag="VF516_01170"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEX8106299.1"
                     /translation="MTLARRIIPCLDVKDGRVVKGINFLSLRDAGDPVEQAAAYDAQG
                     ADEVCYLDISASPEGRSTLLDVVARTADQVFAPLTVGGGVRSVEDAERLLDAGADKIA
                     INTAAIRNPDLVAAAAGRFGSQAIVVAVDARRVPGAAPVRWEVYSHGGRTPEGLDALA
                     WCRRVAELGAGEILLTSMDRDGTGAGYDTELLRAVASSVSIPLIASGGVGVLDHLAEG
                     LEAGADAVLAASIFHFGQHTIGEAKAFLAARGLEIRR"
     gene            complement(866..1408)
                     /locus_tag="VF516_01175"
     CDS             complement(866..1408)
                     /locus_tag="VF516_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106300.1"
                     /translation="MSTPVTHGELQDTLGLFQQELRMERQREIQAAIAPLATKADLAP
                     LATRAELHQEIQAAIAPLSTRAELEMWGGALVARIQQGEQRLGNLEHGQQQLIGRLDQ
                     LEQRVSEGFQQTEQRFQQLEQRLLDELARHTSAVLESMQTQIKASFEPLADLPGRVRR
                     LETAVFGPKPLDRPTRRRKR"
     gene            1835..2473
                     /locus_tag="VF516_01180"
     CDS             1835..2473
                     /locus_tag="VF516_01180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106301.1"
                     /translation="MLDDRRLGRRAREVRSPTRQRTTSGFAAVFDGTAPWLARMTNEI
                     HARRARSTVEHGLAPAGTSTAPDPDMMTKLTSILLGTCLIASAGCAHESGTGAAVGAG
                     AGGIIGAAAGGVGWGLFGAAVGGLLGYGVGQQVEAQNEQRMAEALAAGQPTTWSADQG
                     HVYTLEPQPPYYYQGRQCRRFRLLAHVDGQPREVHGTACQQPNGQWEALNTY"
     gene            2672..3340
                     /gene="hisIE"
                     /locus_tag="VF516_01185"
     CDS             2672..3340
                     /gene="hisIE"
                     /locus_tag="VF516_01185"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HEX8106302.1"
                     /translation="MLAEPTYDERGLVPCIVQDAVTDVVLMLAWMNAEALRRTRETGA
                     VHFWSRSRQALWKKGETSGHTLAVAEIRIDCDLDAVLVRARPAGPTCHTGAMSCFFHR
                     DAEGDEGANDAVDDGPALPILHRLAKVIEARRAATAEKSYTKSLLDAGMPKILAKIAE
                     EHGELAAELPSGEDAKVVHETADLLFHVMVGLAARRIPLEAVLAELTRRFGTSGHVEK
                     AQRK"
     gene            3337..>4258
                     /locus_tag="VF516_01190"
     CDS             3337..>4258
                     /locus_tag="VF516_01190"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF018444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEX8106303.1"
                     /translation="MSAPGGDVLAPGGGLAGAIPHYEDRAEQRMMSAAVARALADERA
                     LIVEAGTGTGKTLAYLVPALASGKRVVVSTGTRALQDQIARHDVPLLRAILARPFSAV
                     VLKGVANYACRRKLAELAARSASRAAPDVSIASLVDWAAHSETGDRAEVEWLAEAAPL
                     WADATTTPEARVGPRCPYFTRCFVTQARRLADQAQLVLVNHHLYLADRALRAGSPGAR
                     ILPDHDAVIFDEAHQLEDVATEHFAARVSTHRIAELVRDAQIALAAMPLWTGRAAEDT
                     IRAVERAGIALFSLVRAALVAGDAESRGGTG"
BASE COUNT          586 a         1440 c         1649 g          583 t
ORIGIN      
        1 cgcgcgctgt acgacggcaa gttcaccgtc gccgatgccg cgcgcaccgc gcatggcgac
       61 gacgcggtgg cccccggagc cccatgaccc tcgcccgccg catcatcccg tgcctcgacg
      121 tcaaggacgg ccgcgtcgtg aagggcatca acttcctgtc gctgcgcgac gccggcgatc
      181 cggtcgagca ggcggcggcg tacgacgcgc agggcgccga cgaggtgtgc tacctcgaca
      241 tctcggcgtc gccggagggc cgctcgacgc tgctcgacgt ggtcgcgcgc accgcggatc
      301 aggtgttcgc gccgctcacc gtcggcggcg gggtgcgcag cgtcgaggac gccgagcggc
      361 tgctcgacgc cggcgccgac aagatcgcga tcaacaccgc ggcgatccgc aacccggacc
      421 tcgtggccgc cgcggccggg cggttcggca gccaggcgat cgtcgtcgcg gtcgacgccc
      481 gccgcgtgcc gggtgccgcg ccggtgcggt gggaggtgta cagccacggc ggccgcacgc
      541 cggaggggct cgacgcgctg gcctggtgcc ggcgggtcgc cgagctcggc gccggcgaga
      601 tcttgctcac cagcatggac cgcgatggga ccggcgccgg ctacgacacc gagctgctgc
      661 gcgcggtcgc atcgtcggtg tcgatcccgc tgatcgcgtc gggcggcgtc ggcgtgctcg
      721 atcacctggc cgaggggctg gaggccggcg ccgacgccgt gctcgcggcg tcgatcttcc
      781 acttcggcca gcacacgatc ggcgaggcca aggcgttcct cgcggcgcgc ggcctcgaga
      841 tccgccgctg accgcgctcc tgcggtcaac gctttcggcg tcgagttggc cggtcgagtg
      901 gcttgggtcc gaacaccgcg gtctccagcc ggcggacgcg gcccggcaga tccgcgagcg
      961 gctcgaagct cgccttgatc tgcgtctgca tcgattcgag gaccgccgag gtgtggcgcg
     1021 caagctcgtc gagcaaccgc tgttcgagct gctggaaacg ttgctcggtc tgctggaatc
     1081 cctcggagac gcgttgctcg agctgatcga ggcgtccgat cagctgctgt tgcccgtgtt
     1141 ccaggttgcc gaggcgctgc tcgccttgct ggatgcgcgc caccagggct ccaccccaca
     1201 tctccagctc ggccctggtg gagagtggag cgatggcggc ctggatctcc tggtggagct
     1261 cggccctggt ggcgagtgga gcgaggtcag ccttggtggc gagtggagcg atggcggcct
     1321 ggatctctcg ctggcgctcc atccgcagct cctgctggaa cagcccgagg gtgtcctgca
     1381 gctcgccatg cgtcaccgga gtcgacatat ccgtggagcc catcatcact acacctcccc
     1441 tcggtcgaag gcaagacagg gcgcgtgcgg cgcgctcggt gcggcgcacg cacgagcttt
     1501 gttgctccgc agtcgcgcgt gctttcgcgg agaagggatc gagaagcagc gttcgacgac
     1561 aagacgatgt ggaagatgca tgcgccctgg cacagcaaga cgcttgccga cccgcgacgg
     1621 cctgattccg cgaggttcga agacgaagcg tccggccgcc ggacgggatg tccgaaatcc
     1681 ggccggacac cggacatgga tccagccctg cgccgggtcg aatcgccgtc gcggcggccg
     1741 tccgcgaggt ccggcagatg tggatcccga gggcgcacga gagcggcgcg agcgagtcgc
     1801 tccggcgatc acgacggctc cggacggatc cctggtgctc gacgatcggc ggctcggccg
     1861 cagggccagg gaggtgcgca gcccgacgcg gcagcgcacc acgtcggggt tcgccgcggt
     1921 gttcgacggc acagcgccgt ggctcgcgag gatgacgaac gagatccacg cacgtcgagc
     1981 gcgctcgacc gtcgagcacg ggctggcgcc ggcgggcacg tccactgcac ctgatccgga
     2041 catgatgacg aagttgacat ccatcctgct gggaacgtgt ctgatcgcga gcgccgggtg
     2101 cgcgcacgag agtggaacag gcgccgcggt cggcgcgggc gccggcggca tcatcggtgc
     2161 ggccgcgggt ggcgtcggtt gggggttgtt cggtgccgcg gtcggtggat tgctcggcta
     2221 cggcgtgggc cagcaggtcg aggcccagaa cgagcagcgc atggcggagg cgctcgcggc
     2281 gggccagccg acgacctggt ccgccgacca ggggcatgtc tacaccctcg agccccagcc
     2341 gccctactac taccaggggc gccagtgccg caggttcagg ttgctcgccc atgtcgacgg
     2401 ccaaccccgc gaggtgcacg gcaccgcatg ccagcagccc aatggccagt gggaggcgct
     2461 gaacacgtac tagcccgggt cgcagccgac ggcgtggtga acgttccggg caggggcggc
     2521 ctccgggcgg gcggcggcgg gcgagcgtgc ggatacacgt cgcggccgcg gacgtcggga
     2581 tgtgcgcctg gcgatggctg gttgcggcgg tccagaaccg ttctcggcgc cagaagttga
     2641 catggtgatc cacgctggcg aacgtaggtc gatgctggcc gagccgacgt acgacgagcg
     2701 tggcctggtg ccctgcatcg tgcaggacgc cgtcaccgat gtggtgctga tgctggcgtg
     2761 gatgaacgcc gaggcgctgc ggcggacccg cgagaccggc gcggtgcact tctggtcgcg
     2821 gtcgcggcag gcgctgtgga agaagggcga gacgtcgggc cacacgctcg cggtcgccga
     2881 gatccggatc gactgcgatc tcgacgcggt gctggtgcgg gcgcggccgg ccgggccgac
     2941 ctgtcacacc ggggcgatga gctgcttctt ccaccgcgac gccgagggcg acgaaggtgc
     3001 gaacgacgcg gtcgacgacg ggccggcgct gccgatcctg caccggctgg cgaaggtgat
     3061 cgaggcgcgg cgcgcggcga ccgccgaaaa gagctacacg aagtcgctgc tggacgccgg
     3121 catgccgaag atcctggcga agatcgccga ggagcacggc gagctcgcgg ccgagctgcc
     3181 gtcgggcgag gacgccaagg tggtccacga gacggcggac ctgctgttcc acgtgatggt
     3241 cgggctcgcg gcccggcgga tcccgctgga ggcggtgctc gcggagctga cccggcggtt
     3301 cgggaccagc ggtcacgtcg agaaggcgca gcggaagtga gcgcgccggg tggcgatgtg
     3361 ctggccccgg ggggggggct ggccggggcg atcccgcact acgaggaccg ggccgagcag
     3421 cggatgatgt cggcggccgt ggcgcgcgcg ctcgccgacg agcgggcgct gatcgtcgag
     3481 gcggggaccg ggaccggcaa gaccctggcg tacctggtgc cggcgctggc gtcgggcaag
     3541 cgggtcgtgg tgtcgaccgg cacgcgcgcg ctgcaggatc agatcgcgcg ccacgatgtg
     3601 ccgctgctgc gcgcgatcct ggcgcggccg ttctcggcgg tcgtgctcaa gggggtggcg
     3661 aactacgcgt gccggcgcaa gctggccgag ctcgcggcgc gcagcgccag ccgggccgcg
     3721 cccgacgtct cgatcgcctc gctggtcgac tgggcggcgc acagcgagac cggcgaccgc
     3781 gccgaggtcg agtggctggc cgaggcggcg ccgctgtggg cggacgcgac gacgacgccg
     3841 gaggcgcggg tcgggccgcg ctgcccgtac ttcacgcgct gcttcgtgac ccaggcgcgc
     3901 cggctggccg accaggcaca gctcgtgctg gtcaaccacc acctgtacct cgccgatcgc
     3961 gcgctgcgcg ccgggagccc gggggcgcgg atcctgccgg accacgacgc ggtgatcttc
     4021 gacgaggcgc accagctcga ggacgtggcg accgagcact tcgcggcgcg ggtgtcgacc
     4081 caccggatcg ccgagctggt ccgcgacgcg cagatcgcgc tcgcggcgat gccgctgtgg
     4141 accggccgcg ccgccgagga cacgatccgc gcggtcgagc gcgccgggat cgcgctgttc
     4201 tcgctggtcc gcgcggcgct ggtggccggc gatgccgagt cccgcggcgg caccgggg
//
LOCUS       DASVOR010000054         5330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_117518,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000054 DASVOR010000000
VERSION     DASVOR010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5330
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117518"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            864..2312
                     /locus_tag="VF516_01195"
     CDS             864..2312
                     /locus_tag="VF516_01195"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8106304.1"
                     /translation="MAHLLLIDDDAVLVPQQVRQAFPPPAYRVVVAQNGNHGVELVRR
                     APPDVVLLDLHLSDRHGLEVYRSIRAIDARIPVIFIATAKTADAAIETVKHGAFDYLH
                     KPLDLAQLQRVVTDALEVARMRLPVRLADATPHAELDGAMLGASPRMLDIYKAIGRVA
                     AQDVTVLITGESGTGKELVARAIYQHSSRSSAPFLALNCAAIPENLLESELFGHERGA
                     FTGADRRRIGKFEQCNGGTVLLDEIGDMPLALQAKILRLLQDQTFERLGGNETIRTDV
                     RLIASTHRDLRVRAAEDKFRPDLYYRLGVFTIHLPPLRDRLEDLPILVQHYLRRFSAE
                     LGREVREIAPEAMARLRTYPWPGNIRELQSVLRQALLRASGTVLLPAFLPDLSGIPPV
                     RPSTPAIIEPTFDVERFIRRRLAPATHDLSAETHREVDRLLMTLALEYTHGNHRDAAR
                     LLGISRQTMRVKLRALGLHVAHSVEPADDDEP"
     gene            2463..2735
                     /locus_tag="VF516_01200"
     CDS             2463..2735
                     /locus_tag="VF516_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106305.1"
                     /translation="MRAAPPQTTTEQTTAPTDEVSRDPDGVPHDVVGLSALFSFLLAA
                     LVLGMWLTGSTTGRVSAIALLVFGVPMLVSGLRERAERERDRRHSR"
     gene            complement(2992..4908)
                     /gene="ftsH"
                     /locus_tag="VF516_01205"
     CDS             complement(2992..4908)
                     /gene="ftsH"
                     /locus_tag="VF516_01205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8106306.1"
                     /translation="MAAQPRPPAASPPGPSRPSWAWFVLLALLGVFWLVQSSVGTTER
                     PPVEYSTFLQWVRAGKVKEVVVRPDSVSGKLSEAETVDGKGVTEFRTVTPRDDRLVAL
                     LDQKGVQIRAETEDSPLLVRLVMMALPWVVIIGVWLWLSRRTQQMMVAGGGPLGGFLK
                     RGRKFEKAASPRVTFEDVAGLANAKRDLSEIVQFLKQPELFASLGAKIPRGVLLVGPP
                     GTGKTLMARAVAGEADVPFYSISASEFVEMFVGVGAARVRELFTEAKKNAPSIVFIDE
                     LDGVGRARGTGLGGGHDEREQTLNQLLSEMDGFDRGDLVVVLAATNRPDVLDAALLRP
                     GRFDRRVVIDLPEAGARKAILGVHTRDKPLADDVDLEQVAATTAGFSGADLANLANEA
                     ALAATRRHAEAISRADFSAAYDKLVLGDPREGKLRLVEKKRVAVHESGHAVIAWATPE
                     AEPLNRVSILPRGMALGATQQIAPEDRHLHTRAELDARLLVLLGGYAAERVVLGEIST
                     GAENDLGEATRLASKMVAHYGMSEALGPVRYDVHEEHAFLGQRIATESGTSDATVHAI
                     ETEARALLGRALATAIAALTAHRAELDRLAAALLQDETLERDRLDELLGPRTAPPDVL
                     PIQAPVPGPRAASS"
     gene            complement(4951..5256)
                     /locus_tag="VF516_01210"
     CDS             complement(4951..5256)
                     /locus_tag="VF516_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106307.1"
                     /translation="MDLPAGRSLDRECYRRTTRASSEAYQRIAQDRIAATEGPVLLVR
                     EISGAGASVDGLTTPLTRPGCSSAISARRDSCARRARPVQDLRGLAAHAARRARNLR"
BASE COUNT          784 a         1954 c         1807 g          785 t
ORIGIN      
        1 cgcgatggtc gagaccccgg tgaccgcgag gacggccatc gagatcagcg gcatcccggc
       61 gaggaacagg acgaccgaca gcatgctggt gaacgcgaac ccgcacatca cggcggcatc
      121 cgcgctgcct ggctcgacgc gggacgcgac gatcgccgcg gacggcaggg tcatgagagg
      181 tcctgcggga ggctcgtaca tgcggttcac gcccacttct ggcgcgactc gcgcgatgtg
      241 tcggcaacca tgctcaccgt cgttgcacgg gccgagccac cgaccgcgcg cggcgatccg
      301 ggcgagtacg cgcaaaccac tgcggtcacc gtacagtcca cgcaagccat gcgtgatcgg
      361 gcttacgcac cggcgccccg ggcgacccat gatgcagagc gcgcccgggt cgtacagcgg
      421 cgcagggacc gggcgctatg ccccacgcga tctcgacggg accgccggag gcgcccacat
      481 gcagcgcgga cgcggccagg cgcccgatcg cggcccggac atctgcagca tttcgctctg
      541 tatgctggaa tgcgggccaa ttttcgcgtg accgtgaaat cgctgcgatg tccggaacca
      601 ggcgcgctcc gctcaccctc gcggccttcg acgtctcgag tccgaccctt cgaggaaacc
      661 tctccctcgc cgcgccgcct agcagggagt gccgagcgcg gttctacccg gaaatcccac
      721 ggcccggttt cgctccggcg cgccgtggat caccctcgga gatccgtcac aacgatgacc
      781 agcgcctcgt tcgcacagtc cgtcctgctc gcggcgatag cgagtctgac gccggacgtg
      841 caataactgg aacgcagcgg ttcatggcgc acctgttgct catcgacgat gacgcagtcc
      901 tcgtgccgca gcaggtacgg caggcattcc ccccgcccgc gtaccgcgtc gtggtggcgc
      961 agaacggcaa ccacggcgtc gagctggtcc gccgcgcgcc gcccgatgtc gtgctgctcg
     1021 acctgcacct gtcggatcgc cacgggctcg aggtctaccg cagcatccgg gcgatcgatg
     1081 cccggatccc ggtgatcttc atcgccaccg ccaagaccgc cgacgcggcg atcgagaccg
     1141 tcaagcacgg cgcgttcgac tacctgcaca agccgctcga cctggcgcag ctccagcgcg
     1201 tggtcaccga cgcgctcgag gtcgcgcgca tgcggctgcc ggtccggctc gccgacgcga
     1261 cgccgcacgc cgagctcgac ggcgcgatgc tgggcgcctc gccgcggatg ctcgatatct
     1321 acaaggcgat cggccgggtc gcggcccagg acgtcaccgt gctgatcacc ggcgagagcg
     1381 gcaccggcaa ggagctcgtc gcccgcgcga tctaccagca cagctcgcgg tcgtccgcgc
     1441 cgttcctcgc gctcaactgc gcggcgatcc ccgagaacct gctcgagagc gagctgttcg
     1501 gccacgagcg cggcgcgttc accggcgccg atcgccggcg gatcggcaag ttcgagcagt
     1561 gcaacggcgg caccgtgctg ctcgacgaga tcggcgacat gccgctcgcg ttgcaggcca
     1621 agatcctgcg cctgctccag gaccagacct tcgagcggct cggcggcaac gagacgatcc
     1681 gcaccgacgt ccggctgatc gcctcgaccc accgcgacct ccgggtccgc gccgccgagg
     1741 acaagttccg ccccgacctg tactaccggc tcggggtgtt cacgatccac ctgccaccgc
     1801 tgcgcgatcg cctcgaagat ctgccgatcc tcgtgcagca ttacctccgc cggttcagcg
     1861 ccgagctcgg ccgggaggtc cgcgagatcg caccggaagc gatggcgcgg ctgcgcacct
     1921 acccctggcc gggcaatatc cgggagctcc agagcgtttt gcgccaggcg ctactgcgcg
     1981 cgagtggcac agtcctcctc ccggcgttcc tccccgatct gtccggcatc cccccggtgc
     2041 gtccgagcac gccggcgatc atcgagccga ccttcgatgt cgagcgcttc atccgccggc
     2101 ggctcgctcc ggcgacccac gacctctcgg ccgagaccca ccgcgaggtg gatcggctct
     2161 tgatgacgct cgcgctcgag tacacgcacg gcaaccatcg cgatgccgct cgcctcctcg
     2221 gaatttcgag gcagacgatg cgggtaaagc tccgcgcact tggcctgcac gtcgcgcact
     2281 ccgtcgagcc ggccgacgac gacgagccct gacgccgggc caggccgggc tcgtgattga
     2341 gcactgtggc caatttatgg ccacaagtca atcgagattg gctattcatt gaccattcgt
     2401 gcgaccactc gccgcaggta ttgcgacggt acgcgtggca cacggcctgc tgtccggcgg
     2461 gcatgcgcgc agcaccgcca cagaccacca ccgagcagac cacggccccg accgacgagg
     2521 tcagccgcga tccggacggt gttccccatg acgtcgtcgg cctgtcggcg ttgttctcgt
     2581 tcctcctcgc tgcccttgtc ctggggatgt ggctgaccgg aagcacgacg ggccgggtct
     2641 cggccatcgc attactggta tttggcgtgc cgatgctggt ctcggggctg cgcgagcgtg
     2701 ccgagcgcga gcgcgatcgt cgccattcgc gatgatctgc gacgctgggt agtttttgat
     2761 ccggagcctc gaattttcga cagtggctcc gatgcctacc cggctggtgc aacggtccgt
     2821 tgcatcgccg ggtgctgagc ccgccggagc ccctgcaccg gccgaggccg atccgaccgg
     2881 aagggccatt ccagagtgcc aacggttggc acaacgggat tgtggcgcgc gcggagtgga
     2941 tgtgtatccg gcgtgtgtcg agcgggcgtc gcgacggtgt ccgcgcgcgg ctcacgagct
     3001 cgcggcccgc gggcccggga ccggcgcctg gatcggcagc acgtcgggag gtgcggtgcg
     3061 cggaccgagc agctcgtcga gccgatcgcg ctccagcgtc tcgtcctgga gcagcgccgc
     3121 cgccagccga tcgagctcgg cgcgatgcgc ggtcagcgcg gcgatcgcgg tcgcgagcgc
     3181 ccggccgagc agcgcgcgtg cctcggtctc gatcgcgtgc acggtcgcgt cgctggtccc
     3241 gctctcggtc gcgatccgct ggccgaggaa cgcgtgctcc tcgtggacgt cgtagcgcac
     3301 gggaccgagc gcctcgctca ttccgtagtg cgcgaccatc ttggacgcca gccgggtcgc
     3361 ctcgccgagg tcgttctcgg cgccggtcga gatctcgccg agcaccaccc gctcggccgc
     3421 gtagccgccg agcaggacga gcagccgggc gtcgagctcg gcgcgggtgt ggaggtgccg
     3481 gtcctcgggc gcgatctgct gcgtggcccc cagcgccatc ccgcgcggca ggatcgacac
     3541 ccggttgagc ggctcggcct ccggggtcgc ccacgcgatc accgcgtggc ccgactcgtg
     3601 gaccgcgacc cgcttcttct cgaccagccg cagcttgccc tcgcgcggat cgccgagcac
     3661 cagcttgtcg tacgccgcgg agaagtcggc gcggctgatc gcctcggcgt ggcggcgggt
     3721 cgcggcgagc gcggcctcgt tcgccaggtt cgcgaggtcg gcgcccgaga accccgcggt
     3781 ggtcgcggcg acctgctcga ggtcgacgtc gtccgccagc ggcttgtccc gggtgtggac
     3841 gccgaggatc gccttgcgcg ccccggcctc cggcaggtcg atcaccaccc ggcgatcgaa
     3901 ccggcccggc cgcagcagcg cggcgtccag cacgtccggc cggttggtcg ccgcgagcac
     3961 gaccacgagg tcgccgcggt cgaacccgtc catctcggac agcagctggt tcagggtctg
     4021 ctcgcgctcg tcgtggccgc cgcccagccc ggtgccgcgc gcccggccca cgccgtcgag
     4081 ctcgtcgatg aacacgatcg acggcgcgtt cttcttggcc tcggtgaaca gctcgcggac
     4141 ccgcgccgcg ccgacgccga cgaacatctc gacgaactcc gacgcggaga tcgagtagaa
     4201 cggcacgtcg gcctcgccgg cgaccgcgcg cgccatcagc gtcttgccgg tgccgggcgg
     4261 accgaccagc agcacgccgc gcgggatctt ggcacccagg ctggcgaaca gctcgggttg
     4321 cttcaggaac tggacgatct cggacaggtc gcgcttggcg ttggcgaggc cggcgacgtc
     4381 ctcgaacgtc acgcgcggcg acgccgcctt ctcgaacttg cgcccgcgct tcaggaaccc
     4441 gccgagcggc ccaccgccgg cgaccatcat ctgctgggtg cgccgcgaca gccacagcca
     4501 gaccccgatg atcaccaccc agggcagcgc catcatcacc agccgcacga gcagcggcga
     4561 gtcctcggtc tccgcccgga tctggacgcc tttctggtcg agcagcgcga ccagccgatc
     4621 gtctcgaggg gtcacggtcc ggaactcggt cacgcccttg ccgtcgacag tctcggcctc
     4681 ggacagcttg cccgagacgc tgtccggtcg caccacgacc tccttgacct tgccggcgcg
     4741 gacccactgc aggaaggtcg agtactcgac gggcgggcgc tcggtggtgc cgacgctcga
     4801 ctggaccagc cagaacacgc cgagcaacgc gagcaggacg aaccatgccc aggacggtcg
     4861 tgacggcccc ggaggcgaag cagcaggggg gcgaggttgc gcggccatac gctgctgtgg
     4921 agcaagggcc atacctgcga accgccgcgt tcatcgcaag ttacgcgcgc gccgcgccgc
     4981 gtgcgctgcc aggccgcgca gatcctgcac cggtcgcgca cgccgcgcgc agctgtcacg
     5041 ccgcgccgag atcgccgagc tgcaacccgg tcgggtcagc ggtgtcgtga ggccatcgac
     5101 gctcgcgccc gctccgctga tttcccgcac cagcaggacc gggccctcgg tggcggcgat
     5161 ccgatcctgc gcgatgcgct ggtaggcctc cgagctcgcc cgggtggtac gccggtaaca
     5221 ctcccggtcg agcgatcgtc cggcgggcag atccaacgcc ggggccgcgc gttttcatcc
     5281 aggacgacat gcgcgggaat ggttcgatgg cgcgggtcgt tgcccgggag
//
LOCUS       DASVOR010000055         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_263562,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000055 DASVOR010000000
VERSION     DASVOR010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263562"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="VF516_01215"
     CDS             <1..644
                     /locus_tag="VF516_01215"
                     /inference="COORDINATES: protein motif:HMM:NF015963.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX8106308.1"
                     /translation="GFFRSVFIHLAWTDRTVIDRSELVLTLHHELFKHLELEMFVPAS
                     RLRDALAVVRHAIEVFDGSAAAPAAAPDPAVEAALGGIGLLDELRRHAGSYTHHYPIF
                     IRRVLPDDTLISMTAGASEPFYTISLFCYHEPRTAFCTLADFVARALNRLHRACPHWG
                     KYFPLGYADIEEYYPRLAAFRAICERHDPRGTFRNAYVTSVLGFTGAAGERAA"
     gene            complement(672..1049)
                     /locus_tag="VF516_01220"
     CDS             complement(672..1049)
                     /locus_tag="VF516_01220"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX8106309.1"
                     /translation="MSRWLAAGLVASAAACGGTARGLEAYRSDTSALLETRTAQLQRC
                     YSDALKTDARLSGTVTVQFVVEKKTGTITSPSVDQSKSTAPPMLGDCVVKAIDGLTLA
                     PPDRNEGRATFVYEFKPNPAPAS"
     gene            complement(1060..>1607)
                     /locus_tag="VF516_01225"
     CDS             complement(1060..>1607)
                     /locus_tag="VF516_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8106310.1"
                     /translation="AEAKPPPRVEVRDNKIEIHDKIQFDYDKATIRPESFGLMNEIAS
                     VIGKNPQIKKIRIEGNASSEGSPQHNQKLSEDRARSVMKYLTDHGVAAGELVSIGYGA
                     DRPIADNGTDAGREQNRRVEFVILEQDVTHKKVEVDAQTGTEKVVDEKHEVVRAADAD
                     ASKSSAKGAAQPQAAAKKKGG"
BASE COUNT          188 a          593 c          533 g          293 t
ORIGIN      
        1 gtgggttctt ccgctcggtg ttcatccacc tggcgtggac ggaccgcacg gtgatcgacc
       61 gcagcgagct cgtgctgacc ctgcaccacg agctgttcaa gcacctcgag ctcgagatgt
      121 tcgtcccggc cagccggctg cgcgacgcgc tggcggtcgt gcgccacgcg atcgaggtgt
      181 tcgacggcag cgccgccgcg cccgccgccg cgcccgaccc cgcggtcgag gccgcgctcg
      241 gcgggatcgg cctgctcgac gagctgcgcc ggcacgccgg ctcgtacacc caccactacc
      301 cgatcttcat ccgccgggtg ctgcccgacg acacgctgat ctcgatgacc gccggcgcga
      361 gcgagccgtt ctacacgatc agcctgttct gctatcacga gccgcgcacc gcgttctgta
      421 cgctggccga cttcgtcgcg cgcgcgctca accggctgca ccgcgcctgt ccgcactggg
      481 gcaagtactt cccgctcggc tacgccgaca tcgaggagta ctacccgcgg ctcgccgcgt
      541 tccgcgcgat ctgcgagcgc cacgacccgc gcggcacgtt ccgcaacgcc tacgtgacga
      601 gcgtgctcgg cttcaccggc gcggccggcg agcgggcggc ctagatccgc cccggccctg
      661 gcccgcgagc gctacgacgc cggcgccggg ttcggcttga actcgtagac gaacgtcgcc
      721 cggccctcgt tgcgatccgg cggcgcgagg gtcagcccgt cgatcgcctt gaccacgcag
      781 tcgccgagca tcggcggtgc ggtcgacttg ctctggtcga ccgacgggct ggtgatcgtg
      841 ccggtcttct tctcgaccac gaactgcacg gtgaccgtcc cgctcagcct ggcgtcggtc
      901 ttgagcgcgt cgctgtagca gcgctggagc tgcgcggtgc gggtctcgag cagcgcgctg
      961 gtgtcgctgc ggtaggcctc gaggccgcgc gccgttccgc cgcaggcggc cgcgctcgcg
     1021 accaggccgg ccgcgagcca tctcgacatg gcagtcatct catccacctt tcttcttggc
     1081 cgcggcctgc ggctgggccg cgcccttggc gctggacttg ctggcgtcgg cgtccgccgc
     1141 gcggacgacc tcgtgcttct cgtcgaccac cttctcggtg ccggtctggg cgtcgacctc
     1201 gaccttcttg tgggtgacgt cctgctcgag gatcacgaac tcgacccgcc ggttctgctc
     1261 gcggccggcg tcggtgccgt tgtcggcgat cgggcgatcc gcgccgtagc cgatcgacac
     1321 cagctcgccg gcggcgacgc cgtggtcggt caggtacttc atcaccgatc gcgcgcggtc
     1381 ctcggacagc ttctggttgt gctgcgggct accctccgag ctggcattgc cctcgatccg
     1441 gatcttcttg atctgcggat tcttgccgat caccgacgcg atctcgttca tcaggccgaa
     1501 cgactcgggc cggatcgtcg ccttgtcgta gtcgaactgg atcttgtcgt ggatctcgat
     1561 cttgttgtcg cgcacctcga cgcgcggcgg cggcttggcc tcggcga
//
LOCUS       DASVOR010000056         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_322107,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000056 DASVOR010000000
VERSION     DASVOR010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322107"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VF516_01230"
     CDS             <1..1123
                     /locus_tag="VF516_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106311.1"
                     /translation="RMKACLVVSVAMMGCGFPRPPDVNPDAPSAGDAPAAPVCFGKIV
                     PICFPASAVPSAPRTLLTVENDTDLTDTGSLCDQNNDQKAAYCVVAGAGVSLPAGKTV
                     TAHGSKPLILLSTAAMELLGSIDVSSHLTGGPQPRGAGANPTDLTACVFATRAVAATM
                     GGGGAGASFGQQGGGGGNPAGSATGGGIAGSRLDMFPAGLRGGCRGNDGSATAGSPAA
                     GGDGGGAVALIAAMQIQVDASINASGGGGKGGAAGAANGGGGGSGGMIAFDSPQPLVF
                     GANARLWANGGSGGQGGSVTTGGAPGAESDRPNNPGSPVNVSGAGGVGADGAIGDDLG
                     GHGGNALGGGGGGGGGGGVGFIHAPGLTNTASISPSSSG"
     gene            complement(1195..1779)
                     /locus_tag="VF516_01235"
     CDS             complement(1195..1779)
                     /locus_tag="VF516_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106312.1"
                     /translation="MRTFAAMGWTSCDVGSAARREAPQEVADRHGCQEFGVILTGSSV
                     GPRIEREKRGVAIVHEQAAGPDLVEWLCQRDGRGDDQPHVVSKLNQQLRNEHRLAPGG
                     LGKAPAHEVVEADAAKDPADVAALRERQIEQFAGKLEDAELDVPWTAHRVVHAIHERA
                     TVLAEHHHDAGTRLTIRAPSRVVAELRAALQSAS"
BASE COUNT          264 a          609 c          720 g          290 t
ORIGIN      
        1 ccgcatgaag gcgtgcctgg tcgtgtccgt cgccatgatg ggatgcgggt tcccgcgtcc
       61 gcctgacgtg aaccccgatg ctccaagcgc cggcgatgcg cctgccgctc cggtgtgctt
      121 cggcaagatc gtgccgatct gttttccagc gtccgcggtg ccatcggcgc caagaacgct
      181 cttgaccgtc gagaacgaca ccgacctgac ggacactggc tcgctgtgcg atcagaacaa
      241 cgaccagaag gcggcgtact gcgtcgtcgc aggcgccggc gtctcgcttc cagccggcaa
      301 gacggtgacc gcgcacggta gcaagccgct catcctgcta tcgaccgcgg cgatggagct
      361 gctgggcagc atcgacgtca gcagccacct gaccgggggc ccccagcccc gtggggcagg
      421 agccaatccg accgacctga cggcctgcgt gttcgcaaca cgcgcggtgg cggccacgat
      481 gggcggcggc ggtgccggcg ccagcttcgg ccagcagggc ggcggaggcg gcaacccggc
      541 ggggtccgcg acgggtggag ggatcgcggg cagccggctg gacatgtttc cggcgggact
      601 gcgcggtggt tgtcggggca acgacggatc cgcgactgcc ggttctccgg cggcgggtgg
      661 ggatggcggc ggcgcggtcg cgctgatcgc cgcgatgcag atccaggtcg atgcgtcgat
      721 caacgcctcg ggtggcggcg gcaagggcgg agcggcgggc gcggccaacg gcggcggcgg
      781 cggctcaggc ggcatgatcg cgttcgactc gccgcagccg ctggtgttcg gcgcgaacgc
      841 gaggctgtgg gccaacggcg gcagcggcgg acagggcggc tcggtgacca cgggtggggc
      901 gcccggcgcc gagtccgacc gtccgaacaa tccggggtcg ccagtcaatg tctccggcgc
      961 gggcggcgtc ggtgccgacg gcgcgatcgg agacgatctc gggggccacg gaggtaacgc
     1021 cctcggaggg ggcggtggcg gagggggcgg tggcggcgtc ggattcattc atgcgcctgg
     1081 gctcaccaac actgcttcga tctcgccgtc gtcatccgga taggacgacg agaccctgcg
     1141 gacgcgttcg atgcagcggc gcggagctga ccggcggtct cgggcgcgtg agcgctacga
     1201 cgcggactgc agggcggcgc ggagctcggc gacgacgcgg ctgggggcgc ggatcgtgag
     1261 ccgggtgccg gcgtcgtggt ggtgctcggc gagcacggtg gcacgctcgt ggatggcgtg
     1321 gacgacgcgg tgggcggtcc acggcacgtc gagctcggcg tcctcgagct tgccggcgaa
     1381 ctgctcgatc tggcgctcgc ggagcgcggc gacgtcggcg gggtccttgg cggcatccgc
     1441 ctcgacaacc tcgtgcgccg gtgccttccc gaggccgcct ggcgcgagcc tatgttcgtt
     1501 ccgaagctgc tggttcagtt tggatacgac atgcggttga tcgtcgcctc gtccatcgcg
     1561 ctggcagagc cactcgacca gatccggtcc agcggcttgt tcgtgtacga tagcgacgcc
     1621 tcgcttctca cgctcgatgc gtgggccaac cgatgaaccg gtcagaatca cgccgaattc
     1681 ctggcatcca tggcgatctg ccacttcttg cggcgcttcg cggcgggctg cagagccaac
     1741 atcacagctg gtccagccca tggccgcgaa cgtgcgcatg atcgcaggcg gcgcctgacc
     1801 gtccaccgat ctgccacgtg atctcgtggc gtcatgagag catcgcggcg atgcgaaggc
     1861 ttgcgtggct catcgtggga gcg
//
LOCUS       DASVOR010000057         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_351333,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000057 DASVOR010000000
VERSION     DASVOR010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351333"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1380
                     /locus_tag="VF516_01240"
     CDS             <1..1380
                     /locus_tag="VF516_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106313.1"
                     /translation="GVSASGPGLVAGQQLGHFRIDRPLGAGGMGEVYLATDLALDRPV
                     AIKVLPPALARDPTRRDRLIREARAQARVSHPNVGHIYFIGEEGGREAGRLYFAMEFV
                     AGETVAERIARGPVSIEDALAIIRSAALGLREAQRSGITHRDIKPSNLMIDSHGMVKV
                     LDFGLAAGEPGVLGAGPVAQTSLAGTPLYMAPEQARGEPVDFHADIYALGATLFHLVS
                     GRPPFEADTLQALLTKHASAERPLLPRRAGTPRTAVAAIDALIARMMAPAPADRFASY
                     DELIRAIELSSVDHMRPAGFWVRSMAAFVDLMATLLAAGGLYAVVALASRGSHDDAVL
                     DALIFAAYLIGATALVARKGWTPGKWMFELEVVDTATGQRPSLRRAAIRNVALIALPL
                     VAAIVDGGLHLFGVRSGIVMKTLLGVVACAPPFALLWASLRSHGKLTIWDRLSGTMVR
                     YRTRRTTAI"
     gene            1401..>1787
                     /locus_tag="VF516_01245"
     CDS             1401..>1787
                     /locus_tag="VF516_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106314.1"
                     /translation="MPTVPWGSGVVALVGWLAACQPLIEPGAPRAAELDSGDRRVAGR
                     LDDRSIEFAFDGGQLEFELYRDGTRIEQVVRNRYAVPVTLRWSIGGLDNLEASSSLEG
                     TTVLPAAREPLGPGEPVVLAELSQIEP"
BASE COUNT          216 a          657 c          660 g          254 t
ORIGIN      
        1 ggtgtttcag cctccggccc cggcctggtc gccggccagc agctcggcca cttccggatc
       61 gacaggccgc tcggcgcggg cggcatgggt gaggtctacc tcgccaccga tctcgcgctc
      121 gaccggccgg tcgcgatcaa ggtgctgccg ccggcgctcg cccgcgaccc gacgcgccgc
      181 gatcggctga tccgcgaggc ccgcgcccag gcccgggtca gccaccccaa cgtcggccac
      241 atctacttca tcggtgaaga ggggggacga gaggccgggc ggctgtactt cgcgatggag
      301 ttcgtcgccg gcgagaccgt cgccgagcgg atcgcgcgcg gcccggtcag catcgaggac
      361 gcgctggcga tcatccggtc ggccgcgctc gggctgcgcg aggcccagcg ctcgggcatc
      421 acccaccgcg acatcaagcc gtcgaacctg atgatcgaca gccacggcat ggtcaaggtg
      481 ctcgacttcg ggctcgccgc cggggagccc ggcgtgctgg gcgccggccc ggtggcgcag
      541 acctcgctcg ccgggacgcc gctgtacatg gcgcccgagc aggcgcgcgg cgagccggtc
      601 gacttccacg ccgacatcta cgcgctcggc gccacgctgt tccacctggt gtcggggcgg
      661 ccgccgttcg aggccgacac gctccaggcg ctgctgacca agcacgccag cgccgagcgg
      721 ccactgctgc cgcgccgcgc cggcacaccg cgcaccgcgg tcgcggcgat cgacgcgctg
      781 atcgcgcgga tgatggcgcc ggcgcccgcc gaccggttcg cgagctacga cgagctgatc
      841 cgcgcgatcg agctgagctc ggtcgaccac atgcggccgg ccgggttctg ggtccgctcg
      901 atggcggcgt tcgtggacct gatggccacc ctgctggccg ccggcggact ctacgcggtc
      961 gtcgccctgg cctcgcgcgg gtcgcacgac gacgccgtcc tcgacgcgct gatcttcgcg
     1021 gcctacctga tcggcgcgac cgcgctggtg gcgcgcaagg gctggacgcc cgggaagtgg
     1081 atgttcgagc tcgaggtcgt cgacaccgcg accggccagc gcccgtcgct gcgccgcgcc
     1141 gcgatccgca acgtcgcgct gatcgccctg ccgctggtcg ccgcgatcgt cgacgggggg
     1201 ctccacctgt tcggcgtccg gagcggcatc gtgatgaaga cgctgctggg ggtcgtggcg
     1261 tgcgccccgc cgttcgccct gctctgggcg tcgctgcgct cgcacggcaa gctgacgatc
     1321 tgggaccggc tgagcggcac gatggtgcgc taccgcacgc ggcgcaccac ggcgatctag
     1381 cttttgctac gatgagcgca gtgccgaccg ttccttgggg ctccggcgtg gtggcgctgg
     1441 tgggctggct cgcagcgtgt cagccgctga tcgagccggg tgcgccgcgc gcggcggagc
     1501 tcgactccgg cgaccggcgg gtagcgggcc ggctcgacga ccggtcgatc gagttcgcgt
     1561 tcgacggcgg ccagctcgag ttcgagctgt accgcgacgg cacccggatc gagcaggtcg
     1621 tgcgcaaccg ctacgcggtg ccggtcacgc tgcgctggtc gatcggcggg ctcgacaacc
     1681 tcgaggcgag ctcgtcgctc gagggcacga ccgtgctgcc cgcggcgcgc gagccgctcg
     1741 gccccggcga gccggtcgtg ctcgccgagc tgtcgcagat cgagcct
//
LOCUS       DASVOR010000058         2418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_409813,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000058 DASVOR010000000
VERSION     DASVOR010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409813"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VF516_01250"
     CDS             complement(<1..638)
                     /locus_tag="VF516_01250"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8106315.1"
                     /translation="MQRAFVAKLWALAAGAAAAWLLPPGWAAPAIAAIAVALVGFFTF
                     AIAHPRSQFFVPVVDHLASDGPVVALTFDDGPDPVVTPRILDLLGAHGARATFFVLGE
                     RAARYPDVIRRIHREGHTVGTHTQHHRIRFHFARPGHVRREIEDAIDVVAGILPARPA
                     LFRPPQGLRTPNFASGWRRTHGLTCVTWSVRAFDSRPTTADAIVGRVAHRLAP"
     gene            complement(652..1878)
                     /locus_tag="VF516_01255"
     CDS             complement(652..1878)
                     /locus_tag="VF516_01255"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF006587.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HEX8106316.1"
                     /translation="MRRRVVITGIGLASPLGCDLGEAAARLRAGGHGIVAMPEWESID
                     HFAPRVAARALVPEALELPHKAARTMGRVAQLATIATQIAVRDAGLLPDEFADGTIGV
                     AYGSTHGSSQANEEWARKLVKQNGFLGLSSTTYLKFMSHTTAANLATHFGVHGRVICT
                     SAACVSATQAIGHGFETIGAGLADVMLCGGAEELHFTHAAVFDMLHAASKAFNTTPER
                     TPRPFDQRRDGLVVGEGAGTLVLEDLGRARRLGKRIYAEIVGFGTNCDGTHVTNPSAD
                     GMRGSIERALRSAGIGPDAIDYVNAHATGTTIGDIAESQATHAVFGDRVPVSSLKGHL
                     GHTLGACGPIEIALAIAMLQGGWIAPTRNLEAVDPQCAPLDYVRGEPRSAQLTRILCN
                     KFAFGGINTSVVLAAV"
     gene            complement(1875..>2418)
                     /gene="fabG"
                     /locus_tag="VF516_01260"
     CDS             complement(1875..>2418)
                     /gene="fabG"
                     /locus_tag="VF516_01260"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF004200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEX8106317.1"
                     /translation="FDVRDAAAARAAIDELLVQTEIHVVVNNAGVSADAAFPAMSQGT
                     WDAVIDTSLDGFFHVTQPLVMPMVRRRWGRIVNLASVSGVHGNRGQVNYSAAKAGLIG
                     ATRALAIELASRNITVNAVAPGLIDTEMIASAPVDEMLKHIPMKRIGTAQEVADVVAF
                     VVSDRAAYMTGQVLVVSGGL"
BASE COUNT          330 a          864 c          895 g          329 t
ORIGIN      
        1 ggcgccaggc ggtgcgccac ccgcccgacg atcgcgtcgg cggtggtcgg ccgcgagtcg
       61 aacgcgcgca ccgaccacgt cacgcaggtc aggccgtgcg tccggcgcca gcccgacgcg
      121 aagttcggcg tgcgcaggcc ctgcggcggc cggaacagcg cgggccgggc cggcaggatg
      181 ccggcgacca cgtcgatcgc gtcctcgatc tcgcgccgca cgtggcccgg ccgggcgaag
      241 tggaaccgga tgcggtggtg ctgggtgtgg gtgccgacgg tgtggccctc gcggtggatc
      301 cgccggatca cgtccgggta gcgggcggcg cgctcgccga gcacgaagaa cgtcgcgcgc
      361 gcgccgtgcg cgccgagcag gtcgaggatc cgcggcgtga ccaccggatc ggggccatcg
      421 tcgaacgtca gcgcgacgac cgggccatcg ctcgccaggt ggtcgaccac cgggacgaag
      481 aactggctgc gcggatgggc gatcgcgaat gtgaagaacc cgacgagcgc gaccgcgatc
      541 gccgcgatcg ccggcgccgc ccagcccgga ggcaacagcc acgccgccgc ggcgccggcc
      601 gcgagggccc aaagcttggc gacgaacgct ctttgcatcg ccggcctccg ctcacaccgc
      661 cgcgaggacg acgctggtgt tgatgccgcc gaacgcgaac ttgttgcaca ggatgcgggt
      721 cagctgggcg ctgcgcggct cgccccgcac gtaatcgagc ggcgcgcact gcgggtcgac
      781 cgcctcgagg ttgcgggtcg gcgcgatcca gccgccctgc agcatcgcga tcgccagcgc
      841 gatctcgatc gggccgcacg cgcccagggt gtggccgagg tggcccttga gcgagctcac
      901 cggcacgcgg tcgccgaaca ccgcgtgggt cgcctggctc tcggcgatgt cgccgatcgt
      961 cgtgccggtc gcgtgcgcgt tgacgtaatc gatcgcgtcg gggccgatgc ccgccgagcg
     1021 cagcgcgcgc tcgatcgagc cgcgcatgcc gtcggccgat gggttggtga cgtgggtgcc
     1081 gtcgcagttg gtgccgaacc ccacgatctc ggcgtagatc cgcttgccga gccgccgcgc
     1141 gcggcccagg tcctcgagca ccagcgtgcc cgcgccctcg ccgacgacca ggccgtcgcg
     1201 ccgctgatcg aacggccgcg gcgtccgctc gggcgtcgtg ttgaacgcct tgctcgcggc
     1261 gtgcagcatg tcgaacaccg cggcgtgggt gaagtggagc tcctcggcgc cgccgcacag
     1321 catgacgtcg gcgagcccgg cgccgatggt ctcgaagccg tggccgatcg cctgggtcgc
     1381 gctgacgcac gccgccgacg tgcagatcac gcggccgtgg accccgaagt gggtcgcgag
     1441 gttggccgcg gtggtgtggc tcatgaactt gaggtaggtg gtcgacgaca gcccgaggaa
     1501 gccgttctgc ttgaccagct tgcgcgccca ctcctcgttg gcctgcgacg agccgtgcgt
     1561 gctgccgtag gccacgccga tcgtgccgtc ggcgaactcg tcgggcagaa ggcccgcatc
     1621 gcggaccgcg atctgggtcg cgatcgtcgc gagctgggcg acccggccca tcgtgcgcgc
     1681 cgccttgtgc gggagctcga gcgcttcggg gaccagggcc cgcgcggcga cccgcggcgc
     1741 gaaatggtcg atcgactccc actccggcat cgccacgatg ccgtgcccgc cggcgcgcag
     1801 ccgcgcggcc gcctcgccga gatcgcagcc cagcgggctg gcgaggccga tgccggtgat
     1861 gacgacgcgc cgcctcacag cccccccgag acgaccagga cctggccggt catgtacgcg
     1921 gcccggtcgc tgaccacgaa cgcgacgacg tcggcgacct cctgggcggt gccgatccgc
     1981 ttcatcggga tgtgcttgag catctcgtcg accggagccg acgcgatcat ctcggtgtcg
     2041 atcagcccgg gcgccaccgc gttgaccgtg atgttgcggc tggcgagctc gatcgcgagc
     2101 gcccgggtcg ccccgatcag cccggccttg gcagcggagt agttcacctg acctcgattc
     2161 ccgtgcacgc ctgatacgct cgcgaggttg acgatgcgcc cccaccggcg gcgcaccatc
     2221 ggcatgacga gcggctgggt gacgtggaag aagccatcga gcgaggtgtc gatcacggcg
     2281 tcccaggtcc cctgggacat cgccgggaac gccgcgtcgg cggacacccc ggcgttgttg
     2341 actacgacgt ggatctcggt ctggacgagc agctcgtcga tcgccgcgcg cgcggcggcg
     2401 gcgtcgcgga cgtcgaac
//
LOCUS       DASVOR010000059         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_526678,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000059 DASVOR010000000
VERSION     DASVOR010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526678"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VF516_01265"
     CDS             complement(<1..988)
                     /locus_tag="VF516_01265"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX8106318.1"
                     /translation="MTAGRLDALLSPRTVAVVGASPDLRIFRGRTLQVMLGHPYAGRI
                     YPVSRSHAEIQGLRAYPSIAAIPEPIDLAVLIIPAEFVPDELARCAAAGVRAAMILAS
                     GFAEAGTPAGEQLQARVVAIAERHGMIVCGPNAEGYANTAAALCPTFSPAVHHLTVPL
                     VPPWRTRGHVAVVAQSGGMGFAMFDRGRARELPFSYIISTGNEACLETFEIVDHLLDD
                     GRTDVFLLYVETVRDVPRFERVAARALAAGKPIIAVKTGKTPAGVRAARAHTGAIAGD
                     PAECQALFRRHGILEVDDLDEMVDLAAAFSLHGDRLPAGTRVGIGSASGGGGG"
     gene            complement(985..2142)
                     /locus_tag="VF516_01270"
     CDS             complement(985..2142)
                     /locus_tag="VF516_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8106319.1"
                     /translation="MTDAIHFDQVRLPEPAQALRREVRRFLADEIAAGTFDPRRGATL
                     GAFHPEFSRKVGARGWIGMTWPRRYGGGERSFLERYVVTEEFRVANAPVGLHFTADRQ
                     SGPILLRYASERIKADILPRITRGECCFCIGMSEPNSGSDLFAAQTRAIRCDGGWRVT
                     GTKVWTTAAHRADYMIGLFRTSPRTAEDRRHGLTQFLVDLRSPGIAISPISDMSGRRE
                     FDEVVFDDMFVPEDHALGEIDGAWKQATSELAYERSGPERFLETSYLLFELVRVLGPR
                     PDVRGAEGLGRLVAQLHALRRMSVSVAGMLEAGQEPTLEGSIVKDLGTTWQQTLPPRA
                     RDLTALLPPTADRAVFDALLDYATLAAPKLTIQGGTTEILRGIIARGLGLR"
     gene            complement(2147..>2488)
                     /locus_tag="VF516_01275"
     CDS             complement(2147..>2488)
                     /locus_tag="VF516_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8106320.1"
                     /translation="AAVALVVSGSAADALAAWAPEATPWAIAYHDSLLLEVASAKIRV
                     GEAAAAGAAIAHQVHGAIGFSTEHPLHRFTRRLWAWRDDYGSETVWAARLGAHVAERG
                     GDALWPMLAAR"
BASE COUNT          334 a          899 c          948 g          307 t
ORIGIN      
        1 agccgccgcc gccgcccgac gcgctgccga tgccgacccg ggtgccggcc ggcagccggt
       61 cgccgtgcag cgagaacgcc gccgcgaggt cgaccatctc gtcgaggtcg tcgacctcga
      121 ggatgccgtg gcggcggaac agcgcctggc actcggcggg gtcgccggcg atcgccccgg
      181 tatgcgcccg tgcggcgcgc accccggcgg gggtcttgcc ggtcttgacc gcgatgatcg
      241 gcttgccggc ggcgagcgcg cgggccgcga cgcgctcgaa ccgcggcacg tcccgaacgg
      301 tctcgacgta gagcaggaac acgtcggtcc ggccgtcgtc gagcaggtgg tcgacgatct
      361 cgaaggtctc caggcacgcc tcgttgccgg tcgagatgat gtagctgaac ggcagctcgc
      421 gggcgcggcc gcgatcgaac atcgcgaacc ccatgccgcc gctctgcgcg accaccgcga
      481 cgtggccgcg ggtgcgccat ggcgggacca gcggcacggt caggtggtgg accgccgggg
      541 agaacgtcgg gcacagcgcc gcggcggtgt tggcgtagcc ctcggcgttg gggccgcaca
      601 cgatcatgcc gtggcgctcg gcgatcgcga cgacccgggc ctgcagctgc tcgccggccg
      661 gcgtgccggc ctcggcgaac cccgaggcca ggatcatcgc cgcccggacc ccggcggcgg
      721 cgcagcgcgc gagctcgtcg gggacgaact cggccgggat gatcagcacc gcgaggtcga
      781 tcggctcggg gatcgcggcg atcgacgggt acgcgcgcag accctggatc tcggcgtggc
      841 tgcggctgac cgggtagatc cggccggcgt aggggtggcc gagcatcacc tggagcgtgc
      901 ggccgcggaa gatccggaga tcgggcgacg cgccgaccac cgcgacggtg cgcggcgaca
      961 gcagcgcgtc gagccgaccg gccgtcatcg caaccccagg ccgcgggcga tgatgccgcg
     1021 caggatctcg gtggtgccgc cctggatcgt gagcttgggc gcggcgagcg tcgcgtagtc
     1081 gagcagcgcg tcgaacaccg cgcggtccgc ggtggggggg agcagcgcgg tcagatcgcg
     1141 ggcccgcggc ggcagggtct gctgccaggt cgtgccgagg tccttgacga tcgagccctc
     1201 gagcgtcggc tcctggccgg cctcgagcat cccggcgacc gacaccgaca tccggcgcag
     1261 cgcgtggagc tgcgcgacca gccggccgag gccctcggcg ccgcgcacgt cgggccgcgg
     1321 gccgagcacg cggaccagct cgaacaggag gtacgaggtc tcgaggaacc gctccgggcc
     1381 gctgcgctcg taggccagct cgctggtcgc ctgcttccac gcgccgtcga tctcaccgag
     1441 cgcgtggtcc tcgggcacga acatgtcgtc gaacaccacc tcgtcgaact cgcggcggcc
     1501 gctcatgtcg gagatcgggc tgatcgcgat gcccggtgac cgcaggtcga ccaggaactg
     1561 ggtcagcccg tgccggcggt cctcggccgt ccgcggcgac gtccggaaca ggccgatcat
     1621 gtagtcggcg cggtgcgccg cggtggtcca caccttggtg ccggtgaccc gccagccgcc
     1681 gtcgcaccgg atcgcccggg tctgggcggc gaacaggtcg gagcccgagt tgggctcgct
     1741 catgccgatg cagaagcagc actcgccgcg cgtgatccgc ggcaggatgt cggccttgat
     1801 ccgctcgctg gcgtagcgca agaggatcgg cccgctctgg cggtcggcgg tgaagtgcag
     1861 gccgaccggg gcgttggcga cccggaactc ctcggtgacg acgtagcgct cgaggaagct
     1921 gcgctcgccg ccgccgtagc gcctgggcca ggtcatgccg atccagccgc gcgcgccgac
     1981 cttgcggctg aactcggggt ggaacgcgcc gagcgtcgcg ccgcgccgcg gatcgaacgt
     2041 gccggcggcg atctcgtcgg cgaggaaccg gcggacctcg cggcgcagcg cctgcgccgg
     2101 ttccggcaac cgcacctgat cgaagtggat cgcgtcggtc atgggatcac cgcgccgcca
     2161 gcatcggcca cagcgcatcg ccgccgcgct cggcgacgtg ggcgccgagc cgggccgccc
     2221 acaccgtctc gctgccgtag tcgtcgcgcc acgcccacag ccggcgggtg aagcggtgca
     2281 gcgggtgctc ggtcgagaac ccgatcgcgc cgtgaacctg gtgggcgatc gcggcgccgg
     2341 ccgccgcggc ctcgccgacc cggatcttgg cgctggcgac ctcgagcaac aggctgtcgt
     2401 ggtaggcgat cgcccagggc gtcgcctcgg gcgcccacgc cgccagcgca tcggccgccg
     2461 agcccgacac aaccagcgcc accgcggc
//
LOCUS       DASVOR010000060         9253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_409821,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000060 DASVOR010000000
VERSION     DASVOR010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9253
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409821"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="VF516_01280"
     CDS             complement(<1..771)
                     /locus_tag="VF516_01280"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEX8106321.1"
                     /translation="MEPGDLVLRGGRVFDPAAARPVDQAGDVRIAHGRIVEIGRGLVG
                     GRILDVRDLWIVPGLIDLHVHLREPGQEYKEDIETGTRAAAAGGFTTICCMPNTRPVN
                     DQRTVTELIVRRSREIGAVRVRPIGAITAGLEGKALADIGDMKEGGIVAISDDGRPVM
                     NAGLLRRALEYARTFDLPVVQHAEDLDLAEGGAMNEGAVATRIGIKGQPVCAESAMVA
                     RDLEIVEWTGARYHVAHASTARTVALVRDAKRRGLPVTC"
     gene            complement(859..2247)
                     /gene="hisS"
                     /locus_tag="VF516_01285"
     CDS             complement(859..2247)
                     /gene="hisS"
                     /locus_tag="VF516_01285"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEX8106322.1"
                     /translation="MPVQPRTTPGVLELLPPQQLAFQHMLDTIRRGFERFGFMPIETP
                     VFELSDVLLTKTGGETEKQVYFVQSTGALRQSGQAGSSEAGKLPELALRFDLTVPLAR
                     YVAEHEAQLNFPFRRYQIQRVYRGERNQKGRFREFYQCDIDVIGKDHLPLAYDAEIPA
                     VIHTIFSELAFGPFTIHLNNRKVLRGLLTAFGVDAGGHEAVLHEIDRLGKLPRAEIEV
                     RLTGEGFGLDPDRVTRLLDLMTGHAGDSRAALAALGRIERPDPLLVEGIDELRRVYDG
                     TLALGVPERALQVDLALVRGLDYYTGSIYETLLDEHPQLGSICGGGRYDNLAGHYTKS
                     KLPGVGISIGATRLFSRLLELGLITAARTAVAHVLVLAVEPTLAAECARIAAELRAAG
                     LNVEVYGGDDKLAKQLKYADRSGIPLAVLYGSRERDAGLVKLKDLRATAATKEHDVPR
                     GEIAARVQQLVG"
     gene            complement(2256..4304)
                     /locus_tag="VF516_01290"
     CDS             complement(2256..4304)
                     /locus_tag="VF516_01290"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEX8106323.1"
                     /translation="MDQTGKSDPRRRPAPDRAVDDKPKPASDATSAAPGTPPIPSLPK
                     GGGAIRGIGEKFSANPVTGTASLQIPIATTPGRAGLHPDLSLAYDSGAGNGPFGHGFH
                     LSVPQIARKTDKGLPQYDDSDVFILSGAEDLVPRLRADAGWQRERFDDGAEAVERYVP
                     RVEGLFARIEKREHRPTGAVYWQATTKDNVTSTYGRSEQARIADPADPRRVFSWLLEE
                     TRDDRGNVTRYDYKAEDLVHVPRAVPWEANRHAGDAPIVNRYLKRIRYGNTAPFDATP
                     GLDSAVFEVVFDHGEHDADAPTVDEARPWPCRQDPFSSYRAGFEVRTYRLCRRVLMFH
                     RMAELGAAPCLVRSTDLAYAENPALSQLVAATHAGYLRDPATLAYTRKSYPPVELSYA
                     LPQLQTEIRTLDPASAADLPEGVQGAYQWTDLDGEGLPGVLSQQGDALYYQQNLGGGL
                     LAPARPLLRQPSLARLGASGQQITDLDGDGRKELAIFTPPAAGYFDRTDDGDFEPFRT
                     FPSQAFVDWSDPNLRHIDLDGDGHQDLLVARAETFTWYPSLAKGGHGPPITFSKPRDD
                     EKGPALVFADGTQSIFLADMAGDGLTDLVRIRNGSVCYWPNLGYGRFGAKVEMGGETR
                     FDHPAVTDAVTWTSLDRLDSLIAPRRQQAALDCSAEKYILRVTQTGSPACHGGSAGSW
                     "
     gene            4486..4782
                     /locus_tag="VF516_01295"
     CDS             4486..4782
                     /locus_tag="VF516_01295"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8106324.1"
                     /translation="MQSLLDRITSSWHPREIWLFGSRARGTAAADSDWDLLVVVPDEL
                     APPGFDDPMAVWNVKQGTGVRADVLVCRASEFDEDRETHNTIAYDAAVEGVRLS"
     gene            4779..5216
                     /locus_tag="VF516_01300"
     CDS             4779..5216
                     /locus_tag="VF516_01300"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX8106325.1"
                     /translation="MSAERVIANLLRIAREDLDGARLLNTRRNRNAIYLREQAAEKLI
                     PAVLTSEKVHAGVRHQLDEMVGLVPEQNPVKPRLRDLQHLSVYATSYRYTTPTGRIPD
                     DPAPEAVEATANRIEGTLLEVARRFGVDLDAADKPAANATPIR"
     gene            5481..5783
                     /locus_tag="VF516_01305"
     CDS             5481..5783
                     /locus_tag="VF516_01305"
                     /inference="COORDINATES: protein motif:HMM:NF017603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HEX8106326.1"
                     /translation="MSAGDEAARDGEENPAERLSLADAAQRALEQFAALSGLQPESLT
                     GVKQVDGGWSLLVDVVEVERVPSTMSVLASYRVDANARGDLISYERLRRFTRVTAD"
     gene            5868..6149
                     /locus_tag="VF516_01310"
     CDS             5868..6149
                     /locus_tag="VF516_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein"
                     /protein_id="HEX8106327.1"
                     /translation="MLERVLDRGVVIAGDIVVSVVNVELLTLKLRLIIASVETAKEIG
                     LDWWMHDPFLNSRARTIELEAENRRLRERLDALEPQREAGTSPPAGERS"
     gene            6146..6904
                     /locus_tag="VF516_01315"
                     /pseudo
     CDS             6146..6904
                     /locus_tag="VF516_01315"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
     gene            6897..7097
                     /gene="gvpJ"
                     /locus_tag="VF516_01320"
     CDS             6897..7097
                     /gene="gvpJ"
                     /locus_tag="VF516_01320"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HEX8106328.1"
                     /translation="MTEETPRSRQLQTASLVDLLDRVLGRGAAVSGDLVIALAGIDLL
                     RLDLRLLLAPVETALGEPQGRP"
     gene            7094..7399
                     /locus_tag="VF516_01325"
     CDS             7094..7399
                     /locus_tag="VF516_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein K"
                     /protein_id="HEX8106329.1"
                     /translation="MTEPDSGSRRISLDAEDVGQGLGRLVVAILDLVRQLLERQALRR
                     VEAGSLGPEEIERLGQALLGLEERLSGLRESLGVSEHGLALPFEIADLDATTGRRTP"
     gene            7396..7749
                     /gene="gvpJ"
                     /locus_tag="VF516_01330"
     CDS             7396..7749
                     /gene="gvpJ"
                     /locus_tag="VF516_01330"
                     /inference="COORDINATES: protein motif:HMM:NF012944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0012506 - vesicle membrane [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gas vesicle protein GvpJ"
                     /protein_id="HEX8106330.1"
                     /translation="MTMAANRYSRPSGLAEILDKGMVIDAFVRVSLVGIEVLTIDARI
                     VVASVDTYLRYAEAVHRLDLTPDGQPRKLTRVVRELREGDSRARAALSHVKETASDLI
                     GSVRDKVTGRHGEHE"
     gene            7746..8492
                     /locus_tag="VF516_01335"
     CDS             7746..8492
                     /locus_tag="VF516_01335"
                     /inference="COORDINATES: protein motif:HMM:NF018129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GvpL/GvpF family gas vesicle protein"
                     /protein_id="HEX8106331.1"
                     /translation="MSAALLVCGVVRSGHPVPLGTPAPDDAIASVRFVHAGGLAAVVR
                     DLPDDAVPGEDDASRHVDALIALLRGGPVLPIRFGTLAPDEDAVRELLEGAGDELARR
                     LDALDGLVEVRLQIDGDLDAELQALVEASPALARVRPERLDDRVRRGEQISARLAERR
                     DELGEQVLARLQRLAVAHTTLRSQIATEVKHAYLIRAEALHEFDEAVQTLRDELGERY
                     AIEYAGPLPPFEFTELSHGAQAPARSRWGW"
     gene            8539..8823
                     /locus_tag="VF516_01340"
     CDS             8539..8823
                     /locus_tag="VF516_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106332.1"
                     /translation="MNSEQLRHRVHDGHSSDRHHDEHPMDRLHSILLERVESSDYPSL
                     ELMDRVEQTLSDRDQAVRYAHVLFDKITRYPSLHILDRLAALIARIEDDC"
     gene            8953..>9253
                     /locus_tag="VF516_01345"
     CDS             8953..>9253
                     /locus_tag="VF516_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106333.1"
                     /translation="MTSQAPDLWDDAVRRHDRRVYLSVLALGLAPERAREITQAAWTR
                     LIEQHQRGALDDIELPGLAVRQARFLAFNELSRTRVESRVLAAVPDPPAAPDTERV"
BASE COUNT         1310 a         3214 c         3367 g         1362 t
ORIGIN      
        1 gcaggtcacc ggcagcccgc gccgcttggc gtcgcgcacc agcgccaccg tccgcgccgt
       61 cgacgcgtgc gcgacgtggt agcgcgcgcc ggtccactcg acgatctcca ggtcgcgcgc
      121 gaccatcgcg ctctcggcgc acaccggctg gcccttgatg ccgatccggg tcgcgaccgc
      181 gccctcgttc atcgccccgc cctcggcgag gtcgaggtcc tcggcgtgct gcaccaccgg
      241 cagatcgaac gtccgcgcgt actccagcgc gcggcgcaac aggcccgcgt tcatcaccgg
      301 gcggccgtcg tcggagatcg cgacgatgcc gccctccttc atgtcgccga tgtcggccag
      361 cgccttgccc tccagcccgg cggtgatcgc cccgatcggc cggacccgca ccgccccgat
      421 ctcgcgcgac cgccgcacga tcagctcggt caccgtgcgc tggtcgttga ccggccgggt
      481 gttcggcatg cagcagatcg tcgtgaaccc gccggccgcc gcggcccgcg tcccggtctc
      541 gatgtcctcc ttgtactcct gcccgggctc gcgcaggtgg acatgcagat cgatcagccc
      601 cggcacgatc cagaggtcgc ggacgtcgag gatccgcccg ccgaccagcc cgcggccgat
      661 ctcgacgatc cggccgtgcg cgatccggac gtcgcccgcc tgatcgacgg gacgcgccgc
      721 cgccgggtcg aacacccggc cgccgcggag cacgagatcc ccgggttcca tcggtgtgcg
      781 gtcttagcag ccgcgacgag cggcgtaaag cccgaccggc gtgcgccgca cgcagcgatc
      841 tccgaacaat cctcgctcct acccgacgag ctgctggacc cgggccgcga tctcgccgcg
      901 cggcacgtcg tgctccttgg tggccgcggt ggcgcgcaga tctttgagct tgaccaggcc
      961 ggcgtcccgc tcgcgggacc cgtacagcac ggcgagcggg atcccggaac ggtcggcgta
     1021 cttgagctgc ttggcgagct tgtcgtcgcc gccgtagacc tcgacgttga ggccggcggc
     1081 gcgcagctcg gccgcgatcc gcgcgcactc cgccgccagc gtgggctcga cggcgagcac
     1141 caggacgtgg gcgaccgcgg tgcgcgcggc ggtgatcagg ccgagctcca gcagccggct
     1201 gaacaaccgg gtcgcgccga tcgagatccc gacgccgggg agcttcgact tcgtgtagtg
     1261 gccggcgagg ttgtcgtagc gcccgccgcc gcagatgctg ccgagctggg ggtgctcgtc
     1321 gagcaaggtc tcgtagatcg acccggtgta gtagtccaga ccgcggacca gcgcgaggtc
     1381 gacctgcagc gcgcgctcgg gcacgccgag ggcgagcgtc ccgtcgtaga cccggcggag
     1441 ctcgtcgatg ccctcgacca gcagcggatc gggccgctcg atccgcccga gcgccgcgag
     1501 cgccgcgcgc gaatcccccg cgtggccggt catcaggtcg agcagccggg tcacacggtc
     1561 tggatcgagc ccgaagccct cgccggtgag ccggacctcg atctcggcgc gcggcagctt
     1621 gccgagccga tcgatctcgt gcagcaccgc ctcgtggccg ccggcgtcga ccccgaacgc
     1681 ggtgagcagg ccgcgcagga ccttgcggtt gttgaggtgg atggtgaacg ggccgaacgc
     1741 gagctccgag aagatggtgt ggatcaccgc cgggatctcg gcgtcgtagg ccagcggcag
     1801 gtggtccttg ccgatcacgt cgatgtcgca ctggtagaac tcgcggaacc ggcccttctg
     1861 gttgcgctcg ccgcggtaca cgcgctggat ctggtagcgc cggaacggga agttgagctg
     1921 ggcctcgtgc tcggcgacgt agcgcgccag cggcacggtc agatcgaagc gcagcgcgag
     1981 ctcgggcagc ttgccggcct cgctggagcc ggcctggccc gattgcctga gcgcgccggt
     2041 cgactgcacg aagtagacct gcttctcggt ctcgccgccg gtcttggtga gcagcacgtc
     2101 ggacagctcg aacaccgggg tctcgatcgg catgaacccg aaccgctcga agccgcgccg
     2161 gatcgtgtcg agcatgtgct ggaacgcgag ctgctgcggc ggcaagagct cgagcacacc
     2221 gggcgtggtt cggggctgga caggcatggc tacgcttacc acgacccggc gctaccgccg
     2281 tgacacgccg gcgagcctgt ttgcgttacc cgaaggatgt atttttcagc ggagcagtcg
     2341 agcgcggctt gctggcgccg tggtgcgatg agagagtcaa ggcggtccag ggaggtccag
     2401 gtgacggcgt ctgtgactgc gggatggtcg aagcgggtct cgccgcccat ctcgaccttg
     2461 gcgccgaacc ggccgtagcc gaggttcggc cagtagcaga cgctgccgtt gcggatgcgg
     2521 accagatcgg tgaggccgtc gccggccatg tcggcgagga agatcgactg cgtgccgtcg
     2581 gcgaacacca gcgcaggccc cttctcgtcg tcgcgcggct tgctgaaggt gatcggcggg
     2641 ccgtggccgc ccttggcgag cgacgggtac caagtgaacg tctcggcgcg cgcgacgagc
     2701 agatcctggt ggccgtcgcc atcgagatcg atgtgccgca ggttggggtc gctccagtcg
     2761 acgaacgcct gcgacgggaa cgtacggaac ggctcgaagt cgccgtcgtc ggtgcggtcg
     2821 aagtagccgg cggccggcgg ggtgaagatc gccagctcct tgcgcccgtc gccgtcgaga
     2881 tcggtgatct gctggccgct cgcgccgagg cgggcgagcg acggctgccg gaggagcggg
     2941 cgcgcgggcg cgagcaggcc gccgccgagg ttctgctggt agtacagcgc gtcgccctgc
     3001 tggctgagca cgccggggag gccctcgccg tcgaggtcgg tccactggta ggcgccctgc
     3061 acgccttcgg gcagatcggc ggcgctggcg ggatcgagcg tgcggatctc ggtctggagc
     3121 tgaggcagcg cgtagctgag ctcgacgggc ggatacgatt ttcgggtgta ggcgagcgtc
     3181 gcggggtcgc ggaggtagcc ggcgtgggtg gccgcgacga gctggctgag cgccggattc
     3241 tcggcgtacg cgaggtcggt cgagcggacg aggcacggcg ccgcgcccag ctcggccatg
     3301 cggtggaaca tgaggacgcg gcggcacagc cggtaggtgc gcacctcgaa gccggcgcgg
     3361 tagctggaga acggatcctg gcggcagggc caggggcgcg cctcgtcgac ggtgggcgcg
     3421 tcggcgtcgt gctcgccgtg gtcgaagacg acctcgaaca cggccgagtc gaggccgggc
     3481 gtggcgtcga acggcgcggt gttgccgtag cggatgcgct tcaggtagcg gttgacgatc
     3541 ggcgcgtcgc cggcgtggcg gttggcctcc cagggcacgg cgcgcgggac atggacgagg
     3601 tcctcggcct tgtagtcgta gcgcgtgacg ttgccgcgat cgtcgcgggt ctcctcgagc
     3661 agccaggaga acacacggcg cgggtcggcc gggtcggcga tgcgcgcctg ctccgagcgg
     3721 ccgtaggtgc tggtgacgtt gtccttcgtc gtggcctgcc agtagacggc gccggtcggc
     3781 ctgtgctcgc gcttctcgat gcgcgcgaac aacccctcga cgcgcggcac atagcgctcg
     3841 acggcctccg cgccgtcgtc gaagcgctcc cgctgccagc ccgcgtcggc gcggagcctg
     3901 ggcacgaggt cctcggcgcc ggagagaatg aacacgtcgg agtcgtcgta ctgcggcagc
     3961 cccttgtcgg tcttgcgggc gatctgcggc accgacaggt ggaagccgtg gccgaagggg
     4021 ccgttgccgg cccccgagtc gtaggcgagc gacaggtcgg gatgcaggcc ggcgcggccc
     4081 ggggtggtgg cgatggggat ctggagcgac gcggtcccgg tgaccgggtt ggcggagaac
     4141 ttctcgccaa tgccacggat cgcgccgccc cccttgggca gcgacgggat cggtggcgtc
     4201 ccgggtgccg cgctcgtggc atcgctcgcg ggcttcggtt tgtcgtcgac agctcggtcg
     4261 ggcgcaggtc tgcgccgcgg atcgctcttg ccggtctggt ccatcgtccc ccctccagct
     4321 tgccgatcat acatcgcgcg ggcgcggcgg tgaggagtcg atgggttcgc ggtcgcatga
     4381 cctggccagg actgctacga ttcgagcatg tcggtcgcag gatccccggc tcgggcccat
     4441 ctcccgccga gggatgcgcg gggcgctccg atcgatctgc atgcggtgca gtcgttgctc
     4501 gaccggatca cctcgagctg gcatccacgg gagatctggc tgttcggtag ccgggcgcgc
     4561 ggcaccgccg cggcggacag cgactgggac ctcctcgtgg tcgttcccga cgagctcgcg
     4621 ccgccaggat tcgacgaccc gatggccgtg tggaacgtga agcaagggac cggcgtccgc
     4681 gccgacgtgc tcgtgtgccg cgcgagcgag ttcgacgagg atcgtgaaac tcacaacacg
     4741 atcgcgtacg acgcggccgt cgagggtgtt cggctctcgt gagcgccgag cgtgtgatcg
     4801 ccaacctgct gcgaatcgcg cgcgaggatc tcgacggcgc aaggctgctg aacacgcgcc
     4861 gtaaccggaa cgcgatctat ctccgcgagc aggcagccga gaagctcatc cctgccgtgc
     4921 tgacgtcgga gaaggttcac gccggcgtgc ggcatcaact ggacgagatg gtcggcctgg
     4981 ttcccgaaca gaacccggtg aagccgcggc tccgtgacct ccagcatctc agcgtctacg
     5041 cgacgtccta ccgatacacc acaccgaccg ggcgcattcc ggacgaccct gcccccgagg
     5101 cagtggaagc cacggccaac cgcatcgagg gcacgctgct cgaggtcgcc cgccggtttg
     5161 gcgtggatct cgatgcagcc gacaagcccg ccgcgaacgc cacgccgatc cggtgacagc
     5221 gtcgcgcctt gatcggcggc cgcgtcaggg catggctacg ctgaccacga ctcggcgatg
     5281 cgcccggggc gcccacgccg cggacgagcg cgcgccgcgt gcacggaccg cggcgtgcgc
     5341 cgtcaacgtc cggccgtgcg ccgaaccgca gagtggaggt catcgctcgg ctcgcccgct
     5401 gcgacgagct cgacggtgat gtcggagcaa cgccgcggct tcagccttgg cgccgcgacc
     5461 gaccatgcta cctaggcagc gtgtcggctg gagacgaggc agcgcgcgat ggcgaggaga
     5521 acccggccga gcgcctcagc ctggcagacg ccgcccagcg cgcgctggag cagttcgcgg
     5581 cgctcagcgg gcttcagcca gagagcctca ccggcgtgaa gcaggtggac ggcggctggt
     5641 cactcctcgt cgatgtcgtc gaggtggagc gcgtcccctc gacgatgagc gtcctggcga
     5701 gctaccgggt cgacgccaat gcccgcggcg atctgatctc gtacgagcgc ctgcgccgct
     5761 tcacacgggt gacggcggat tgatgcatcc cggcaagcgt catgcagacc ctatccacat
     5821 ccggctcgcg ctatgcgacg gggcatcgcc ccgactcgct cgctgacgtg ctggagcgcg
     5881 tcctcgatcg cggtgtcgtc atcgcgggcg acatcgtcgt gagcgtcgtg aacgtcgagc
     5941 tcctgaccct gaagctccgg ctcatcatcg cctcggtcga gaccgccaag gagatcgggc
     6001 tcgactggtg gatgcacgat ccgttcctca actcgcgcgc acgcacgatc gagctcgagg
     6061 ccgagaaccg gcggctccgc gagcggctgg atgcgctgga accccagcgc gaggccggga
     6121 cgagcccgcc ggccggagag cgctcgtgac gcccgcgcgc gggctgtacc tctacgccct
     6181 ggtcccgcac gacgcgcggc tcggcggtgt gcgcggcgtc gccggcgagc cggtcgagat
     6241 cctgcggcac gtgcggctcg gactggtcgc cggcgaggtg agcgtcgaga tgctcgccgg
     6301 cctggagggc gagtcgtccg atcccgggac cctcggcgcc ctcgctcgag gtcacgacga
     6361 ggtcgtgcgt gccgcatggg cggccgcgga tgccgtgctg ccgttccggc tcggcacggt
     6421 cctggtcgat cgggacgccg cacggcgata cctcgaggaa cgagccgagg agatctaccc
     6481 ggcgatgcag caggtcgccg cctgccacga gttgtgagtg gccttccacg accgcgccgc
     6541 cgagatccgg gcgcacgcgg acccgatcgg ctcccacgac ccagggcatc ccggaacggc
     6601 ctacctcgcg cggcgccggc aggaggtcgc gagcgccgag gctcaccggc gcgcgcgacg
     6661 gcaggcgatg tcccacgtgg aggacgagct gcgggcgagg gcgatcgacg tcgccgccgg
     6721 ccgctaccga cagcctggcg tggtgctcga cgagagctac ctggtccggc gcgacgcgga
     6781 gccggcgttc ttcgagacgg tcgatcgctg cggcgacctg ctgctcgagc aaggtttgca
     6841 cctccagctc agcggcccgt ggccgccgta ttcgttcgcg ctccggggag ctggccgtga
     6901 ctgaggagac cccgcgatcc cggcagctcc agaccgcgag cctcgtcgat ctgctcgacc
     6961 gggtcctcgg ccggggcgcc gcggtcagcg gcgacctcgt catcgcgctg gcggggatcg
     7021 atctcctccg tctcgatctg cgactcctcc tcgcgccggt cgagaccgcg ctcggagagc
     7081 cacagggtcg tccatgaccg agcccgactc cggatcacgg cggatctccc tcgacgccga
     7141 ggacgtcggg cagggcctcg gacgtctcgt ggtcgcgatc ctggatctgg tccgccagtt
     7201 gctggagcgt caagcgctgc ggcgggtcga ggccggctcg ctcggtcccg aggagatcga
     7261 gcgcctcggg caggcgctgc tcggcctgga agaacggctc tccgggctgc gcgagagcct
     7321 cggcgtctcc gagcacggcc tggccctgcc cttcgagatc gccgatctgg atgcaacgac
     7381 cggaaggagg acaccatgac gatggcagcg aaccggtatt cacgaccgag tggcctggcc
     7441 gagatcctcg acaaggggat ggtgatcgac gcgttcgtcc gcgtgtcgct ggtcgggatc
     7501 gaggtcctca cgatcgacgc gcgcatcgtg gtcgccagcg tcgacacgta cctgcgctac
     7561 gccgaggcgg tgcaccggct cgatctcact cccgacgggc agccgcgcaa gctgacccgg
     7621 gtggtgcgcg agctgagaga aggcgattcc agggcgcgag ccgcgctctc gcacgtcaag
     7681 gagaccgctt ccgacctgat cggctcggtg cgcgacaagg tcaccgggag acacggcgag
     7741 cacgaatgag cgcggcgctc ctggtctgcg gcgtcgtccg ctccgggcat ccggttccgc
     7801 tcgggactcc ggcgcccgac gacgcgatcg cgagcgtgcg cttcgtgcat gccggggggc
     7861 tcgccgccgt cgtccgcgac ctgcccgacg acgcggtccc aggcgaggac gatgccagcc
     7921 gccatgtcga cgccctgatc gcgctgctcc gcggcgggcc cgtcctgccg atccggttcg
     7981 gcaccctggc tcccgatgag gatgccgtcc gcgagctgct cgagggcgcc ggcgacgagc
     8041 tcgcccgccg gctggacgcg ctcgatggcc tcgtcgaggt gcggctccag atcgacgggg
     8101 acctcgatgc cgagctccag gcgctcgtcg aggcatcccc ggcgctggcc cgcgttcgcc
     8161 cggaacggct ggacgaccgc gtacggcgag gcgagcagat cagcgcacgg ctggccgagc
     8221 gccgggacga gctcggcgag caggtgctcg cgcgcctcca gcggctcgcc gtcgcacata
     8281 cgacccttcg ctcgcagatc gcgacggagg tgaagcacgc gtatctgatc cgcgccgagg
     8341 cgcttcacga attcgacgag gccgtgcaga ccctgcgcga cgagctcggc gagcgctatg
     8401 cgatcgagta cgcggggccg ctgcctccct tcgagttcac cgagctgtcg cacggcgcac
     8461 aggcgcccgc tcgctcgcgc tggggctggt gaggcggggc cggtatgcgt gttgcttcga
     8521 tcctgcgatg ggaaggtcat gaactctgaa caactgcgcc accgcgtcca cgatggacat
     8581 tcgagcgacc gccaccacga tgaacatccg atggaccgcc tccacagcat cttgctcgag
     8641 cgcgtcgaga gcagcgacta cccgagcctg gagctcatgg atcgcgtcga gcaaacgctg
     8701 tccgaccgcg accaggcagt ccggtacgcg catgtcctgt tcgacaagat cacgcggtac
     8761 cccagcctgc acatcctgga tcggctggcg gcgctgatcg cgcgcatcga ggatgactgc
     8821 tagatcgtca tccctccggc gcgcgtgccg tgtgcaaggc gctggccaag cggcccgcca
     8881 cccgccggac cgcatccagc gaatctcccc gatatgatcg cggcgcccgc ttgttcttgg
     8941 ggggtgcacg cgatgacgag ccaggctccc gacctgtggg atgacgcggt ccgccggcac
     9001 gatcgccggg tctacctctc ggtgctcgcg ctcggcctgg ctccggagcg cgcccgcgag
     9061 atcacgcagg cggcgtggac ccggctgatc gagcagcacc agcgcggcgc gctcgacgac
     9121 atcgagctgc ccgggctcgc ggtccggcag gcgcggttcc tcgcgttcaa cgagctgtcg
     9181 cgcacccggg tcgagagccg ggtgctcgcc gcggtgcccg acccgcccgc ggcgcccgac
     9241 accgagcggg tgg
//
LOCUS       DASVOR010000061         7738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_292928,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000061 DASVOR010000000
VERSION     DASVOR010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292928"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..282)
                     /locus_tag="VF516_01350"
     CDS             complement(100..282)
                     /locus_tag="VF516_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106334.1"
                     /translation="MIRVERFECLGDQARNFRVNAQDLPEGWNIEGLVGLSFLRQFNY
                     EVRSTEGRIRIERAVS"
     gene            complement(315..503)
                     /locus_tag="VF516_01355"
     CDS             complement(315..503)
                     /locus_tag="VF516_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106335.1"
                     /translation="MKVARFSPKSDLIIVKGKLRGPLNPDGRLLRLAFDTGAAETIFA
                     PEVLDELGYNPRQGEAIT"
     gene            complement(500..805)
                     /locus_tag="VF516_01360"
     CDS             complement(500..805)
                     /locus_tag="VF516_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106336.1"
                     /translation="MLASDMSSTPVTITAHERLTWDEICQRYPDTWVVAVSPKRTDET
                     DDADDVTIEFCTAVVVAHHKNRKSLTPFIKAEQHRYEELGTYFTGRLISPSYELVMR"
     gene            937..1137
                     /locus_tag="VF516_01365"
     CDS             937..1137
                     /locus_tag="VF516_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106337.1"
                     /translation="MLDADQGTPGVGYASTSETLAASDGSVEPALQGRQVGARFDARV
                     VVLAGLLLVAALGLVRRRRAQR"
     gene            1459..4458
                     /locus_tag="VF516_01370"
     CDS             1459..4458
                     /locus_tag="VF516_01370"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106338.1"
                     /translation="MSQSSLEDDPQPCHAPGPSHADARAETIDLRRGLIASGTASGTA
                     SGTASRTDATVRAPRVVETLAALGVPDQISTQPSPITDPVAPMSDACPPGSRLGQYEI
                     VRQIGRGGMGTVYLARDLRLGRLVAVKLLSRLGFQDNARFLTEARVTARCNHENIVVI
                     HDVGEHDGQPYMVFEYVAGQTLRAWLDDRTHRSGDSVTPIEPGLAASLMIPVARALAY
                     AHGMGIVHRDLKPANIMLTDAGTIKVLDFGIAKLIAGDDPAGLAFHLPLATEPGVIMG
                     TLPYMSPEQLEGGAVDHRTDLWAVGIMLYQMVTGAHPVIHDGANLQRALLDIAALDLA
                     MPRVGERRSDLGPLAGIIDRCLLKDRAHRTRDARVLLEELEAIAAGRGVAVRGHDGNP
                     FAGLASFQESDADRFYGRSREIGAVVARLRSCPLVALAGPSGVGKSSLVRAGVIPVLK
                     GSGEGWDTFIVRPGRNPLASLCSILLDLSTSSSVPVGEGAPGPHAHAANLRAAPGQLG
                     AALRAHAHSKRRRVLVFIDQFEELYTLCADPGERAAFLASLDGVADDAGSPLRVLVSI
                     RSDFLDRLAEHRDLADRISHGLMFVPPIDRDGLREALVRPVEVADCRYEDPGMVAEML
                     EVVAAAPGSLPLLQFAAARLWAERDRDRRLLTRASYAAMGGIAGTLAGHADHVLGGIP
                     AQERPLVRAIFERLVTPERTRAVVSLGELRELPGNPDDIERLIYRLVDARLLVVEARV
                     GDDRVTELVHESLIGGWPTLIGWLDENREDAAFLARLRTAAAQWEASDRDDGVLWRDE
                     PARQALAWRAHYRGELGRRERAYLDAVRDVATRVERRRRLVRRRILVTVVAVPVILLV
                     VAGVALIRIKRAELDEREISAELRQQKQQLSEKTHQLETANHQLREQEAVKSELLKET
                     QRASEQAELQRDLARRHQEAAERARDEANRATRQARIEKTRAEQAAHEAEQARAAAAA
                     AAEDVRQSEQVKKAIIERAVGPIQRGL"
     gene            4458..5468
                     /locus_tag="VF516_01375"
     CDS             4458..5468
                     /locus_tag="VF516_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8106339.1"
                     /translation="MQRQCHHRSIACSALAALTLALAPIAAHADIARTGAEPWYQQST
                     PDARREAQALFAQAVDKHQQLLRGNARDLYDRALKLWDNPDIQWNLSLVLEDLGQYLP
                     AYQQLDSALRWGEALGAERLRQVRARMQALETHRLARIEASSDEPGAEIMLDGQPWFR
                     GAGRQSQLVEPGAHYVASRKTGFFPVTRPISVTAGQAARVVLSMDADRVIETRRWPAW
                     KPWVVVSAGVLIASVGAGLERQALVHRDAATKSLAGPCDRPAGCMPMAFPTSYDRIVT
                     DSRLAAGAFLAGGTAVAVGLTLAWLNQLHVHRTEARAPGPIEVTPILSTGQVGVSAQL
                     RF"
     gene            5483..7147
                     /locus_tag="VF516_01380"
     CDS             5483..7147
                     /locus_tag="VF516_01380"
                     /inference="COORDINATES: protein motif:HMM:NF040941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX8106340.1"
                     /translation="MRNQILWTVVIELHVAAAVTAGGCLPSLGVACGSGWCSDEEICL
                     SLQAAQPVCIRGTCGNGHLDSGEECDDWNHTGGDGCSPNCMIERCGDGIMQTGEQCDD
                     GNTIDGDGCSASCTTELCGNGILDRAHGEQCDDGNTIDGDGCSAHCRLPSCGTGSLDL
                     YEQCDDGNTIDGDGCSANCTIEICGNGILDRAYGEQCDDGNTIDGDGCSASCRLPSCG
                     NGSLDLYEQCDDGNTIDGDGCSANCTTEVCGNGILDHGEQCDDSNTANGDGCSANCTI
                     EACGNDILDLGEQCDDGNTTDGDCCSAHCTLEFCGNNSCKVDKDCAPAGICDTGGLCR
                     VARSCAEILQHFPASKDGVYSIAPGTTAPFSTVCDMTRDRGGWTLLLKATGDTTLGYS
                     ASAWTDDSLLNASDLTTQPGNAKYRSFLSLPVTTLRGELDGFRYTKNFPGKTAQQIFQ
                     GPDDYVNGFPTFNSGALYWSTQPFCQIFGVNTPFTHTQTRFGWSANEQNDCTSNDTAI
                     GLGLMNDGADHQLGTDQRGAGYECLGPGCKPGFVDTGGHGLLWGHE"
     gene            7422..7589
                     /locus_tag="VF516_01385"
     CDS             7422..7589
                     /locus_tag="VF516_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106341.1"
                     /translation="MSQKAQQDQDRRRAPTQKVKTQDTPITGLSPDDDIGVDSGNTGK
                     PPAPPDPTPTP"
BASE COUNT         1228 a         2636 c         2680 g         1194 t
ORIGIN      
        1 gggggctgca gcacctcggg cggccctccc ggcctgctgg tcgtggcggc gctcggcctg
       61 gtgcgccggc ggcggcggag gcgccaggct ggcggtagct cacgagaccg cgcgttcgat
      121 gcggatccgg ccctcggtcg agcgcacctc gtagttgaac tggcgcagga agctgagccc
      181 gaccaggcct tcgatgttcc agccctccgg tagatcctgg gcgttgacgc gaaagttgcg
      241 cgcttggtcc cccagacact cgaatcgctc gacgcggatc atgtagccat gctcacggcc
      301 aacgactgaa cgcatcacgt gatcgcctcg ccttgacgtg ggttgtagcc gagttcgtcg
      361 aggacctcgg gagcgaagat cgtctcggcc gcgcccgtgt cgaacgcgag ccgaagtagt
      421 cgaccgtcag ggttgagcgg tcctcgaagc tttcccttga cgatgatcag atcgctcttg
      481 gggctgaagc gtgcgacttt caccgcatca ccagttcgta ggacggtgat atgagccttc
      541 ctgtaaagta cgtaccaagt tcttcgtaac ggtgctgctc tgccttgata aacggagtga
      601 ggctcttgcg attcttgtga tgagcgacga ccactgccgt gcagaattcg atcgtgacgt
      661 catccgcatc atctgtctcg tcggtccgct tgggactcac cgcgacgacc cacgtgtccg
      721 ggtagcgttg gcagatctcg tcccaggtca gccgttcatg cgcggtgatt gtaacgggcg
      781 tgctggacat atcgctggcg agcatcgtgg ttccaccctg cgcttccgga aacgcctgtc
      841 aagtttttcg aacaatcctg ggtgtcctcg acgagcttcc gcttcgcaac cgacgcgagg
      901 ctgcagcgtc gtcgcgcatc agcgtgtcgg ctcggcgtgc tcgacgccga ccagggcacg
      961 cccggggtcg gctatgccag cacgtccgag acgctggccg cgagtgatgg ctcggtcgag
     1021 ccagcgctcc agggtcgcca ggtcggtgca cgcttcgatg cgcgcgtcgt cgtccttgcc
     1081 ggcctgctgc tcgtggcggc gctcggcctg gtgcgccgac ggcgggcgca gcgctgaggc
     1141 ccgccgggct gcggtctgac cagcatcacg gcctgcgcgt ccgtgttcaa tccacgaact
     1201 ggacacgtcc agggaaggct gtgcgtgtcc agcgcgtgcc cctcccgacc gcctctgcat
     1261 cacagggcac cggacggagg ttcggccgtg ttccccgcgc tccgacaatc cgaggtcgca
     1321 tcgagggatc gcgccgcgcc ggtggcgtga tcaacccggt ccaccggacg ttgtccgtcg
     1381 agctccgtac ggctcgtggc ttgcacaacg aacagccagt cgcggtggta gtcgacgctg
     1441 cgcgcatcgg acatcgtcat gtcgcaaagc agcctggagg acgatcctca gccgtgtcat
     1501 gcaccggggc ccagccacgc ggacgcgcgg gccgaaacga tcgacctccg ccgtggcctc
     1561 atcgccagcg ggactgccag cgggactgcc agcgggaccg ccagtcgcac cgacgcgacc
     1621 gtccgcgcgc cgcgcgtcgt cgagaccctc gcagcgctcg gcgtgcccga tcagatctcg
     1681 acccagccat cgccgatcac cgatccggtc gcgccgatgt ccgacgcatg cccgcccggc
     1741 agccggctcg ggcagtacga gatcgtccgg cagatcggcc ggggcggcat ggggacggtc
     1801 tatctcgccc gcgatctgcg actgggccgg ctggtcgcgg tcaagctgct gtcacggctc
     1861 ggcttccagg ataacgcgag gttcctcacc gaggcccggg tcaccgcgcg ctgcaaccac
     1921 gagaacatcg tcgtgatcca cgacgtcggc gagcacgacg ggcagccgta catggtgttc
     1981 gagtacgtcg ccggccagac gctgcgcgcg tggctcgatg accgtaccca ccgcagcggc
     2041 gacagcgtca cgccgatcga gcccggcctc gccgccagtc tgatgatccc ggtcgcgcgt
     2101 gcgctcgcct acgcgcatgg catgggcatc gtgcaccgcg acctcaaacc cgccaacatc
     2161 atgctcaccg acgccggcac catcaaggtg ctcgacttcg gcatcgccaa gctgatcgcc
     2221 ggcgacgatc cggccggcct cgcgtttcac ctgccgctgg ccaccgagcc cggcgtgatc
     2281 atggggacgc tgccgtacat gtctccggaa cagctggagg gcggcgcggt cgatcaccgc
     2341 accgatctgt gggcggtcgg catcatgctc taccagatgg tcaccggggc ccacccggtg
     2401 atccacgacg gcgccaactt gcagcgcgcc ctgctcgaca tcgcggcgct cgacctcgcg
     2461 atgccccgcg tcggggagcg gcgatccgac ctcggtccgc tcgccggcat catcgatcgc
     2521 tgcctgctca aggatcgagc gcaccgcacc cgggacgcgc gcgtgctgct cgaggagctg
     2581 gaggcgatcg ccgccggacg gggcgtcgcg gtgcgcggcc acgatggcaa tccattcgcc
     2641 ggtctcgcct cgttccagga gtccgacgcc gaccggttct acggccggtc gcgcgagatc
     2701 ggcgccgtcg tggcgcgcct gcgcagctgc ccgctggtcg cgctcgccgg tccgtcgggg
     2761 gtcggcaagt catcgctggt ccgtgccggt gtcatcccgg tgctcaaggg ctcgggcgag
     2821 ggctgggaca cgttcatcgt gcggcccggc cgcaacccgc tggcgtcgct gtgctcgatc
     2881 ctgctcgatc tgtcgacctc gagcagcgtt ccggtcggcg agggcgcccc cggtccccat
     2941 gcgcatgctg caaacctgcg cgcggcgccc ggtcagctcg gcgccgcgct gcgagcgcac
     3001 gcccacagca agcgccgccg cgtgctggtg ttcatcgatc agttcgagga gctgtacacg
     3061 ctgtgcgccg acccgggcga gcgcgcggcg ttcctcgcga gcctcgacgg cgtcgccgac
     3121 gacgccggct cgccgctgcg cgtgctggtg tcgatccgct cggacttcct cgaccggctc
     3181 gccgagcacc gcgatctggc cgaccggatc agccacggcc tgatgttcgt gccgccgatc
     3241 gatcgcgatg gcctgcgcga ggcgctggtc cgcccggtcg aggtcgcgga ctgccgctac
     3301 gaggaccccg gcatggtcgc cgagatgctc gaggtcgtcg ccgcggctcc cggcagcctg
     3361 cccctgctcc agttcgcggc ggcgcggctg tgggccgagc gcgaccgcga ccgccggctg
     3421 ctcacccggg cgagctacgc cgcgatgggc ggcatcgcgg ggacgctggc cggccacgcc
     3481 gaccacgtcc tgggcggcat cccggcccag gagcgtccgc tcgtgcgagc gatcttcgag
     3541 cgcctggtca cgcccgagcg cacccgcgcc gtggtcagcc tgggcgagct ccgcgagctg
     3601 ccgggcaatc ccgacgacat cgagcgcctc atctaccggc tggtcgacgc gcggctgctg
     3661 gtcgtcgagg cgcgcgtcgg tgacgaccgg gtcaccgagc tcgtgcacga gtcgctgatc
     3721 ggcggctggc cgacgctgat cggctggctc gacgagaacc gcgaggacgc ggcgttcctg
     3781 gcgcggctgc gcaccgccgc cgcccagtgg gaggccagcg atcgcgacga tggcgtgctg
     3841 tggcgcgacg agcccgcgcg tcaggcgctg gcctggcgcg cgcactatcg cggcgagctg
     3901 ggtcgccgcg agcgcgcgta cctcgacgcg gtgcgcgacg tcgccacccg cgtagagcgg
     3961 cggcgccgcc tcgttcgccg ccggatcctc gtcacggtcg tcgcggtccc ggtgatcctg
     4021 ctggtagtgg cgggcgtggc gctgatccgg atcaagcggg ccgagctcga cgagcgggag
     4081 atctccgcgg agctgcgcca gcagaagcag cagctgagcg agaagacgca ccagctggag
     4141 acggcgaacc accagctgcg ggagcaggag gcggtgaaga gcgagctgct gaaggagacc
     4201 cagcgagcgt cggagcaagc cgagctccag cgcgacctgg cgcgccgcca ccaggaggcg
     4261 gccgagcgcg cgcgggacga ggcgaaccgg gctacacgtc aagcgaggat cgagaagacc
     4321 cgcgccgagc aggcagcgca cgaggccgag caggcgcgag cggcagcggc ggccgccgcc
     4381 gaggacgtcc ggcagagcga gcaggtgaag aaagccatca tcgaacgggc ggtgggcccg
     4441 atccagcggg ggctgtgatg cagcgacagt gccatcatcg ttcgatcgcg tgctcggcgc
     4501 tggcggcgct gacgctggcg ctggcgccca tcgccgcaca tgcggacatc gcacgcaccg
     4561 gagccgagcc ctggtatcag cagtcgacgc ccgatgcccg gcgggaggca caggcgctgt
     4621 tcgcgcaggc ggtcgacaag caccagcagc tcctgcgcgg gaacgctcgg gacctgtacg
     4681 accgggcgct gaagctgtgg gacaaccccg acatccagtg gaatttgtcc ctggtgctcg
     4741 aggatctggg ccagtacctg ccggcctacc agcagctcga cagcgcgctg cgctgggggg
     4801 aagcgctcgg cgccgagcgg ctgcgccagg ttcgcgcgcg gatgcaggcg ctggagaccc
     4861 atcgcctggc ccggatcgag gcgtccagcg acgagcccgg ggccgagatc atgctcgatg
     4921 gccagccctg gttccgcggt gcagggcgcc agagccagct ggtggaaccg ggagcgcact
     4981 acgtcgcctc ccgcaagacc gggttcttcc cggtgacccg cccgatctcc gtgacggccg
     5041 gccaggcggc ccgcgtggtg ctgtcgatgg acgcggaccg tgtgatcgag acgcggcgat
     5101 ggcccgcgtg gaagccgtgg gtcgtggtct cggccggcgt cctgatcgcc tcggtcggtg
     5161 cgggtctgga gcggcaggcg ctcgtgcacc gggacgccgc cacgaaatcg ctcgccggcc
     5221 cctgcgatcg gccagcgggg tgcatgccga tggcgtttcc caccagctac gatcgcatcg
     5281 tgacggacag ccggctcgcg gccggcgcgt tcctcgcggg cggcaccgcg gtggcggtgg
     5341 gactgacgct ggcgtggctc aaccagctgc acgtgcatcg caccgaggcg cgtgcacccg
     5401 gtccgatcga ggtcacgccc atcctgtcga caggccaggt cggggtctcg gcgcagctac
     5461 gattctgaag gaggggcgat tgatgagaaa ccagatccta tggactgtgg tgatcgaact
     5521 ccatgtcgca gcggcggtga ctgccggtgg gtgtctgcct tcgctgggtg tagcgtgcgg
     5581 gagcgggtgg tgctcggacg aagagatctg cctgagcctg caggcagcgc agccagtatg
     5641 tatacgaggt acctgcggca atggccacct ggattcaggc gaggaatgtg acgactggaa
     5701 ccacacaggc ggtgacggtt gctcgcccaa ttgcatgatc gaacgttgtg gcgatggcat
     5761 catgcaaact ggtgagcaat gcgacgatgg taatacgatt gatggcgacg gctgttcggc
     5821 cagctgtacg accgagctct gtggcaatgg catcctggac cgcgcgcacg gcgagcagtg
     5881 cgatgacggt aacaccatcg atggcgacgg ctgttccgcg cactgcaggc tgccgtcgtg
     5941 tggcaccggc agcctcgacc tctacgagca gtgcgatgac ggtaacacca tcgatggtga
     6001 cggctgttcc gccaactgca cgatcgagat ctgtggcaat ggcatcctgg accgcgcata
     6061 cggcgagcag tgcgatgacg gtaacaccat cgacggcgat ggctgttccg ccagctgcag
     6121 gctgccgtcg tgtggcaacg gcagtctgga cctctacgag cagtgcgatg acggtaacac
     6181 catcgacggc gacggctgct ccgcgaactg cacgaccgag gtctgcggca acggcatcct
     6241 ggatcatggc gagcagtgcg acgacagcaa caccgccaac ggcgacggct gctccgcgaa
     6301 ctgcacgatc gaggcctgtg gcaacgacat cctggatctt ggagagcagt gtgacgacgg
     6361 caatacgacc gacggcgact gctgttctgc ccactgcacc ctcgagttct gtggcaacaa
     6421 ctcctgcaag gtggacaagg actgcgctcc cgcgggcatc tgcgatacgg gggggctgtg
     6481 ccgggtggcg cggagctgcg ccgagatcct gcagcacttc cccgcgtcca aggatggcgt
     6541 gtacagcatc gccccgggca cgacagcgcc gttcagcacg gtctgcgaca tgacccgcga
     6601 ccgcggcggc tggaccctcc tgctcaaggc cacgggcgac accaccctgg ggtatagcgc
     6661 gtcggcctgg accgatgaca gcttgctcaa cgccagcgac ctgaccaccc agccgggcaa
     6721 cgccaagtac cggagcttcc tctccctacc ggtcaccaca ctacgcggcg agctcgacgg
     6781 gttccgctac acgaagaact ttcccggcaa gactgcacag cagatctttc aaggccccga
     6841 tgactacgtg aatggctttc caaccttcaa ctcgggcgcg ctctactggt ccacccaacc
     6901 cttctgccag atcttcggcg tcaacacccc cttcacacac acccagacgc gcttcggctg
     6961 gagcgccaac gagcagaacg actgcaccag caacgacacc gccatcggtc tggggctgat
     7021 gaacgacggc gcggaccacc agctcggcac ggaccagcgc ggcgctggtt acgaatgcct
     7081 ggggcccggg tgcaagcccg gcttcgtcga caccggtggc catggtctgc tgtgggggca
     7141 tgaatgatcg cagccgtacg ttccgtggac gtggcggcgc ggacctcctc gcacctgtag
     7201 gtcctcgcgg tgccccgtcg tgacctcggc gcggtcgtga ctgagtggtc gtgtccagtg
     7261 aacgcatcgg gcgacgtgtc cagcggcgcg ctggacacgc gtggtggccg gcgcgtggat
     7321 gaaatccatc attgtcaaag attgcacggc ccacaggctg gcactggtcc tgcaatccac
     7381 ggtgaatgcc agcgtatcta cagcaactca ggagagtgac catgtcgcag aaggctcagc
     7441 aggatcagga ccggaggcgt gcgccgacgc agaaggtgaa gacgcaggac acgccgatca
     7501 ccggcctgtc tcccgacgat gacatcggtg tcgattccgg caacactggc aagccgccgg
     7561 cgccgccgga tccgacgccg acgccgtgag ctgtcccgat cggccctgga cgacgccgct
     7621 cacggcatgg agccgggctc gcacgtgcgg gcgatggcgg ggacctcgag ccgtcggcgg
     7681 aacctgggat cctgccgcag aggatcaaac cagggatcgc ccagaccggc cggtttgt
//
LOCUS       DASVOR010000062         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_526695,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000062 DASVOR010000000
VERSION     DASVOR010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526695"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="VF516_01390"
     CDS             complement(<1..564)
                     /locus_tag="VF516_01390"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEX8106342.1"
                     /translation="MTRSRPWLLVVLIGAAVGAAFAAVSTYDFVQHFDRQVHNLHCSF
                     IPGVSHSGESGCQVAMMSPYSSVLRGRIWGGVPIALPALSVFAFLVFYALDLALTRRK
                     ADPRATGFLALATVLPALASLVMLLISVTQLGTTCKLCVCIYVASALCLVGGVALWRQ
                     AVRGRGGEPAPVRRGPHDEPAFLGRAKT"
     gene            694..1473
                     /locus_tag="VF516_01395"
     CDS             694..1473
                     /locus_tag="VF516_01395"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX8106343.1"
                     /translation="MCELLGMECNVPTDITFSFSALSARGGGKGPHAHGWGLALYDGN
                     VAQVFREPAPACTSALAGFVRQHPIKTLLAIAHVRKKTRGRVSLANTHPFVRELWGKN
                     FVFAHNGTVKPVARLRLGGFRPMGQTDSEHAFCALLSALQHDFPRGPRSHDDLAAAIA
                     AHAGRIGKLGTFNMLLGDGDQLFARCSTRLHHLIRRAPFRKATLADEDLCVDFAELTT
                     PRDRVAVVATAPLTRDETWIPAEPNTLWVFRRGKLTHTLAS"
     gene            1574..2479
                     /locus_tag="VF516_01400"
     CDS             1574..2479
                     /locus_tag="VF516_01400"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8106344.1"
                     /translation="MAANLYETLGVKRDASVEDIRKAYRKLAKKHHPDINPGNKAAED
                     RFKAVSAAYEVLSDPKRRAEYDEFGDAALQSGFDPARAREYARWQERQAAGGGFGAGG
                     GFGGGGPVEFDFAELFNRRRGPTRGQDIHATFQMDLRQAVEGTEVSVDVPEQGTVRVR
                     IPPGADTGSVIRLPGKGAPGVRGGPPGDLVIETEVRPHPLLRRDGLDLYLTLPVTLDE
                     AYNGANVDVPTFEGMVVLKIPPRSQNHARLRLRGKGIERKSTRGDLFVELDVRMPDRA
                     DDALADALRAASALYGKPVREGLTL"
     gene            2476..>2671
                     /locus_tag="VF516_01405"
     CDS             2476..>2671
                     /locus_tag="VF516_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106345.1"
                     /translation="MTATRFTYRRLVELVDGDDELVVQLVDEGVIEPCGDDRVTVDVD
                     SVLAAQTLRELEVDWSGIPII"
BASE COUNT          410 a         1001 c          928 g          332 t
ORIGIN      
        1 ggtcttggcg cggccgagga acgcaggctc gtcgtggggc ccgcgccgca ccggggccgg
       61 ctcaccgccg cggccgcgca ccgcctggcg ccacagcgcg acgccgccga ccaggcacag
      121 cgcgctcgcg acgtagatgc agacgcacag cttgcaggtc gtgccgagct gggtcaccga
      181 gatcagcagc atcaccagcg acgccagcgc gggcagcacg gtggccagcg cgaggaaccc
      241 ggtcgcgcgc ggatcggcct tgcgccgggt cagcgccagg tcgagcgcgt agaacacgag
      301 gaacgcgaac accgacagcg cgggcaacgc gatcggcacg ccgccccaga tccggccgcg
      361 cagcaccgac gagtacgggc tcatcatcgc gacctggcag cccgactctc cggagtggct
      421 gaccccgggg atgaacgagc agtgcaggtt gtggacctgg cggtcgaagt gctgcacgaa
      481 gtcgtaggtc gagacagcag cgaacgctgc gccgacggcc gcgccgatca gcacgacgag
      541 gagccatggc cgagatcgtg tcatcggagg gaagttatca cctgcgcccg ccggcggccc
      601 agattcgcat cgatcgctgc cagtctggca cggcggggcg ccgcgcctcg taccgcgcag
      661 ccgcgatccc acccggcgct atactccgcg gatatgtgcg agctgctcgg gatggagtgc
      721 aacgtcccga ccgacatcac gttctcgttc tccgcgctca gcgcacgcgg cggcggcaag
      781 ggcccgcacg cccacggctg gggcctcgcg ctctacgacg gcaacgtcgc gcaggtgttc
      841 cgcgagcccg cgccggcatg cacgtcggcg ctcgccggct tcgtccgcca gcatccgatc
      901 aagacgctgc tcgcgatcgc ccacgtccgc aagaagaccc ggggcagggt ctcgctcgcc
      961 aacacccacc cgttcgtccg cgagctgtgg ggaaaaaact tcgtgttcgc ccacaacggc
     1021 acggtcaagc cggtcgcccg gctgcggctc ggcgggttcc gcccgatggg ccagaccgac
     1081 agcgagcacg cgttctgcgc cctgctgtcc gcgctgcagc acgacttccc gcgcggcccg
     1141 cgctcgcacg acgacctcgc cgcggcgatc gccgcccacg ccggccggat cggcaagctc
     1201 ggcacgttca acatgctgct cggcgacggc gatcagctgt tcgcgcggtg ctcgacccgg
     1261 ctgcaccacc tgatccgccg ggcgccgttc cgcaaggcga cgctcgccga cgaagatctc
     1321 tgcgtcgact tcgccgagct gaccacgccg cgcgaccggg tcgccgtggt cgccaccgcg
     1381 ccgctgaccc gcgacgagac ctggatcccg gccgagccga acaccttgtg ggtgttccgc
     1441 cgcggcaagc tgactcacac cctggcgtcc tagccgggcg tcgctgcccg ccggcgcccg
     1501 cccacccggc cggccagggg tttcgccgag cacctggttt tcggggcgcc cgcgttgcaa
     1561 ggtatgccct ggcatggccg ccaacctcta cgagacgctc ggcgtcaagc gggacgccag
     1621 cgtcgaagac atccgcaagg cgtaccgcaa gctcgccaag aagcaccacc cggacatcaa
     1681 tcccggaaac aaggccgccg aggacaggtt caaggcggtc tcggcggcgt acgaggtgct
     1741 gtccgacccc aagcggcgcg ccgagtacga cgagttcggc gacgccgcgc tgcagagcgg
     1801 cttcgacccc gccagggccc gcgagtacgc gcggtggcag gagcgccagg ccgcgggcgg
     1861 cgggttcggc gcaggcgggg ggttcggcgg cggcggaccg gtcgagttcg acttcgccga
     1921 gctgttcaac cgccggcgcg gcccgacccg cggtcaggac atccacgcga cgttccagat
     1981 ggacctccgc caggcggtcg agggcaccga ggtctcggtc gacgtccccg agcagggcac
     2041 ggtccgcgtc cggatcccgc ccggcgccga caccggctcg gtgatccggc tgcccggcaa
     2101 gggcgcaccc ggggtgcgcg gcggcccgcc cggcgacctc gtgatcgaga ccgaggtccg
     2161 gccgcacccg ctgctgcgcc gcgatggcct cgacctctac ctcacgctgc cggtcacgct
     2221 cgacgaggcg tacaacggcg cgaacgtcga cgtgccgacg ttcgagggca tggtcgtgct
     2281 caagatcccg ccgcgctcgc agaaccacgc ccggctccgg ctgcgcggca agggcatcga
     2341 gcgcaagtcg acccgcggcg acctgttcgt cgagctcgac gtccgcatgc cggaccgcgc
     2401 cgacgacgcg ctcgccgacg cgctgcgcgc cgccagcgcg ctctacggaa agcccgttcg
     2461 cgaggggttg acgctatgac cgccacgcgc ttcacctacc ggaggctggt cgagctggtc
     2521 gacggcgacg acgagctggt cgtccagctg gtcgacgagg gcgtgatcga gccgtgcggc
     2581 gacgaccggg tcacggtcga cgtcgacagc gtgctcgccg cccagaccct gcgcgagctc
     2641 gaggtcgact ggtccggcat cccgatcatc c
//
LOCUS       DASVOR010000063         8114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_263609,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000063 DASVOR010000000
VERSION     DASVOR010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263609"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..1206)
                     /locus_tag="VF516_01410"
     CDS             complement(136..1206)
                     /locus_tag="VF516_01410"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEX8106346.1"
                     /translation="MNPQPRIVVMGAGGIGGIVAATLTEVGAAVTAVSTNPAIRAAVD
                     QSGYRVLDDGEHRVVRGWIAPAPEGSYDLCILATQPPNVEEAALTALRHLADDGQVVV
                     LQNGLCEDRIASIVGAGRVIGGIVAWGASMPEPGRYERTAHGGFALGRLSGEIDDDLR
                     RVGELLEAIGPVSLTRNLRGARWSKLALNCAVSALGTIAGDRLGPLVRVRRYRRLALE
                     VMTEAVAVAKAEGVALEKVAGTLDLDWVALSEADKAASASVSLTAKHMLLLAVGLRYR
                     RMRSSMLAAIERKRTPSIDFLNGEVVTRGARHGIATPFNARIVETVWAIARGTRTSSR
                     ETLDAVCLPEDGPAHRPEPRGA"
     gene            1289..2203
                     /locus_tag="VF516_01415"
     CDS             1289..2203
                     /locus_tag="VF516_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8106347.1"
                     /translation="MQITFWGVRGSYPVPGAATVRYGGQTSCVETRTVSGDTLIVDAG
                     TGMRALGNKLLRQAPEPQHHHVLLSHVHWDHIQGLPFFSPAYVPGTKISVYALQTAAD
                     ELHQVIGGITRHEFFPMPLEAVPASFDFHQVEPGVALDIAGFHVTPIALNHPFGSVGY
                     RIDGDGSSWAYVADTAPFTEVLHKQHFLSGLEPLTDDDRAALAAMRDALVRRLVGVDT
                     AVYDTHFLPDEYTQFPHYGHSTPDQALDICCEAGVRRLVLYHHAPSHGDDQMDRIAAE
                     YLARGARRNIEVLTSFEGMTLPIGPEAA"
     gene            2200..3042
                     /locus_tag="VF516_01420"
     CDS             2200..3042
                     /locus_tag="VF516_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8106348.1"
                     /translation="MKIRFWGVRGSFAMSGRDFLRYGGNTSSVELVADAGQRLLIDLG
                     TGATELAKQLMTGEFGKGHGTVPILLTHTHLDHIQGLPFFTPFFIKGNQIRILGAAPS
                     SLSLEATLQDQLSPHYSPLNGLENLAAGVTIETIIPGSTLALPGFEVVTAAVPHGSMW
                     TTAYRIAADGRTVAFLSDVEYPSVDEPLPAALALAQGADLLIHDAMYADADYELSKGW
                     GHSPARAGVVVAERAAAKKLTLFHHNPDATDDMIDEIVARTAARTAIPVFAAAEGHYL
                     DVGE"
     gene            complement(3239..6271)
                     /locus_tag="VF516_01425"
     CDS             complement(3239..6271)
                     /locus_tag="VF516_01425"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEX8106349.1"
                     /translation="MNAQELRTLVSTLAARDTPRTEAQVQADLRQLLIKAPLDLEERD
                     LTDVVLEAQAGGGTKIDIEVGATVIEVKRDLRKGSVRSKAVEQLAGYVRSRQSTTGLR
                     YVGVLTDGAEWRCFHLVDGELVEVSTVAVSSAKPNVDGLLLWLEGVMATKQNVAPTPE
                     SIQARLGAESSAHALDRATLAVLYAQNRNTSSVVMERHLWARLLRTAFGTQFVDTDEL
                     FVEHTLLVNSAEIIAHAVLGLPVEAISPASLLSGEKFEDSGVYGVVEQDFFDWVVEVS
                     GGANFIKTLARRLSRFAWHSVEHDVLKVLYESIIGRATRKKLGEYYTPDWLAEQIVQR
                     TVKAPLEERLLDPACGSGTFLFHAIRRYIAAAETAGLGVAALLDGATDHVMGMDLHPV
                     AVALARVTYLLALGRNRLTDPARGQIQIPVFLGDSMQWGKKRTDRVLSHGQLVIQADD
                     AHELPGLASELRFPNEVLTNSRIFDQLVQELADRAADRKPGTLPPSLKAIFQRLAIPA
                     SSHEAVKATFATMCRLHDQGRDHIWGYYVRNLARPAWLARPENQVDVLIGNPPWLAFR
                     HMPADMQQACKEMNESRELWKGRKWATQQDLSALFVARTIQLYLKRGGRFGFVMPSSV
                     LNGGQYAGFRAGTFPDPEDRVDLLFEVPWDLRRVRPHFFPITASVIRGTRASSPRPLP
                     SDSERWIGDLRPNSRRWGDVENAIRILSHVGDREVSTLSAYHERFAQGATIVPRVLFM
                     VERAPASPLGTPAGREAVRSMRSATEKKPWKELPPLDGVVETEFIRPIYLGESVLPFR
                     ALSRTRAVIPYDKTGLMAGDSERIELFPGLAAWWRPAEELWMKHRSSERLTLLQRLDY
                     MHEFTIQLPVHRQRVVYTKSGMHLSAARLVDRRAIVDHTLYWASVASDEEAHYLCAIL
                     NAEMTTKLVRPKMSYGKDERHIDKYVWQLPIAEFDPSNPLHDEISRIGKMLEEEIARL
                     HLDSNKGFVWHRQVVRRYLATSESGRLLAEAVAKLLSN"
     gene            complement(6754..7443)
                     /locus_tag="VF516_01430"
     CDS             complement(6754..7443)
                     /locus_tag="VF516_01430"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8106350.1"
                     /translation="MDPRTGPDTEPDALWLDSLPVVLRPSREPDFHQDRARRSYLALI
                     DAATALFGTHGYDAVGTPEIAQRAGVSVGTFYRYFADKHEIYLEIARRTMVAGYRKTI
                     ADLGPERFVGRARHETISETIAILFDHVLSQPELTRSFREMSLRDPQVAEVSRAFEQV
                     AVARITTLITALVGRDVVPDPEATAWVLHSAVVEAAYSLAGHRGPPPITAERARQALT
                     DFIERAVFPPA"
     gene            7525..>8114
                     /locus_tag="VF516_01435"
     CDS             7525..>8114
                     /locus_tag="VF516_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8106351.1"
                     /translation="METAAAATFAAPSIEVIESLLDVTYTWGYQETRQKLRDLYDKAV
                     RAQWISDDVLPWDTDVDLGRPMGPENLQPLFGSDIYARMTEAERQKLKTEVFSWTLSQ
                     FMHGEQGALLATAQLVDSVDNIDSKLYAASQVVDEARHVDVYNRYVHSKIGFSYPINP
                     HLRTLLDLILKDSRWDMKFLGMQIMVEGLALAAFGM"
BASE COUNT         1414 a         2732 c         2556 g         1412 t
ORIGIN      
        1 gccggcgagc acgagggctg cgatcgtcgc tgcgacgagc gcgagcgcga gcaggcccgg
       61 gcgaaccaca cccgggtttt cggctcgccg ggcgcgcgtg tcaaggcctg cccccggtct
      121 cctgcctcgg gccgctcagg cgcctcgggg ttccggccgg tgcgccgggc cgtcctcggg
      181 caggcacacc gcgtcgaggg tctcgcgcga cgacgtccgc gtgccccgcg cgatcgccca
      241 cacggtctcg acgatccgcg cgttgaacgg ggtcgcgatg ccgtggcgcg cgccgcgcgt
      301 gacgacctcg ccgttcagga agtcgatcga cggcgtgcgc ttgcgctcga tcgccgccag
      361 catcgagctc cgcatccggc ggtagcgcag cccgaccgcg agcagcagca tgtgcttggc
      421 ggtcaggctg accgacgccg acgcggcctt gtcggcctcg gacagcgcga cccagtcgag
      481 gtcgagggtg ccggcgacct tctccagcgc gacgccctcg gccttcgcca ccgcgaccgc
      541 ctcggtcatg acctcgagcg cgagccggcg gtagcggcgg acccggacca gcgggccgag
      601 ccggtccccg gcgatcgtgc cgagcgccga gaccgcgcag ttgagcgcga gcttgctcca
      661 ccgggcgccc cgcaggttgc gggtcaggga caccggcccg atcgcctcga gcagctcgcc
      721 gacccgccgg agatcgtcgt cgatctctcc cgacagccgg ccgagcgcga acccgccgtg
      781 cgcggtgcgt tcgtagcgcc ccggctcggg catcgacgcc ccccacgcga cgatcccccc
      841 gatcacccgc ccggcgccga cgatcgaggc gatccggtcc tcgcacagcc cgttctgcag
      901 cacgaccacc tggccgtcgt ccgcgagatg gcgcagcgcg gtgagcgccg cctcctcgac
      961 gttgggcggc tgggtcgcca ggatgcacag gtcgtagctg ccctccggcg ccggggcgat
     1021 ccagccccgc accacccggt gctcgccgtc gtcgagcacc cggtagcccg actggtcgac
     1081 cgccgcccgg atcgccgggt tggtcgacac cgcggtcacc gccgcgccga cctcggtcag
     1141 cgtcgcggcg acgatgccgc cgatgcctcc ggcgcccatc acgacgatcc tcggctgcgg
     1201 gttcatgacc cccttccata acaaactccc ccggttttgg gccgagtttt ggggatctcg
     1261 ctccgtcgcg atagaatttc cgcacccgat gcagatcacg ttctgggggg tccggggcag
     1321 ttacccggtg cctggcgctg ccaccgttcg atacggaggc cagacatcgt gcgtcgagac
     1381 gcgcacggtg agcggggaca cgttgatcgt cgacgccggg accggcatgc gcgcgctcgg
     1441 caacaagctg ctgcgccagg cccccgagcc gcagcaccac cacgtgctgc tgtcgcacgt
     1501 gcactgggac cacatccagg gcctgccgtt cttctcgccg gcctacgtcc cgggcaccaa
     1561 gatctcggtc tacgcgctgc agaccgcggc cgacgagctc caccaggtga tcggcggcat
     1621 cacccgccac gagttcttcc cgatgccgct cgaggcggtg cccgcgtcgt tcgacttcca
     1681 ccaggtcgag cccggcgtcg cgctcgacat cgccgggttc cacgtcacgc cgatcgcgct
     1741 caaccacccg ttcggctcgg tcggctaccg gatcgacggc gatggctcgt cgtgggccta
     1801 cgtggcggac accgcgccgt tcaccgaggt gcttcacaag cagcacttcc tgtccgggct
     1861 cgagccgctg accgacgacg accgggccgc gctcgccgcg atgcgcgacg cgctggtccg
     1921 ccggctggtc ggcgtcgaca ccgcggtcta cgacacccac ttcctgcccg acgagtacac
     1981 ccagttccca cactacggcc actcgacgcc cgaccaggcg ctcgacatct gctgcgaggc
     2041 gggcgtgcgc cggctcgtgc tctaccacca cgcgccgtcg cacggcgacg accagatgga
     2101 ccggatcgcc gccgagtacc tcgccagggg cgcccgccgc aacatcgagg tcctgacctc
     2161 gttcgaaggc atgacgctgc cgatcggccc ggaggcggct tgaagatccg gttctggggc
     2221 gtccgtggct cgttcgcgat gtcgggacgc gacttcctgc gctacggcgg caacacgagc
     2281 tcggtcgagc tggtcgccga cgccgggcag cgcctgctga tcgacctcgg gaccggcgcc
     2341 accgagctcg ccaagcagct gatgaccggc gagttcggca agggccacgg caccgtgccg
     2401 atcctcctga cccacaccca cctcgatcac atccagggcc tgccgttctt caccccgttc
     2461 ttcatcaagg gcaaccagat ccggatcctc ggcgccgcgc cgtccagcct gtcgctcgag
     2521 gccacgctgc aggaccagct gtcgccgcac tacagcccgc tcaacggcct cgagaacctc
     2581 gcggccggcg tcacgatcga gaccatcatc ccgggcagca ccctcgcgct gcccggcttc
     2641 gaggtcgtga ccgccgcggt cccgcacggc tcgatgtgga ccaccgccta ccggatcgcc
     2701 gccgatggcc ggaccgtggc gttcctgtcc gatgtcgagt acccgtcggt cgacgagccg
     2761 ctcccggcgg cgctcgcgct cgcccagggc gctgacctcc tgatccacga cgcgatgtac
     2821 gccgacgccg actacgagct cagcaagggc tggggccatt ccccggcacg cgccggggtc
     2881 gtcgtcgccg agcgcgcggc cgccaagaag ctcacgctgt tccaccacaa ccccgacgcg
     2941 accgacgaca tgatcgacga gatcgtcgcg cgcaccgcgg cgcgcaccgc gatcccggtg
     3001 ttcgccgcgg ccgagggcca ttacctcgac gtcggggagt agccgggggg catcgccaca
     3061 gccttcgcag caccagccgg tcgcggacgg tgtgggcgcc ggcggccagc cgatcgccga
     3121 ggcgatcggc tggcgacaat cccggtggac gaagtgtacc aggtcgatgc gaaccggcgg
     3181 acagaccaga tcggcgccta cgtcgtcggc tccgtccgac gctcggcgta gggtacacct
     3241 aattggaaag cagtttcgcg actgcttctg ctaggagtcg gcctgattcg ctcgtagcca
     3301 ggtaacgacg aacaacctga cgatgccaaa cgaacccctt gtttgagtcg aggtgaagac
     3361 gtgcgatttc ttcctcgagc atttttccaa ttcgagaaat ttcatcgtga agagggttag
     3421 aagggtcgaa ttctgcgatc ggcagctgcc agacgtattt gtcgatgtgt cgctcgtcct
     3481 tgccgtaaga catcttcggc cgtacgagct tcgtcgtcat ctcagcattg agaatcgcgc
     3541 ataggtagtg cgcttcttca tctgatgcca cgctggccca atacagcgtg tggtcaacga
     3601 tcgcgcgccg atcgacaaga cgagcagccg acaaatgcat gcccgacttc gtgtagacaa
     3661 cgcgttgccg atgcacggga agctgaatcg taaactcatg catatagtcg aggcgctgca
     3721 gcagcgtcag ccgttcgctg gagcgatgtt tcatccacag ctcctcagcg ggtcgccacc
     3781 aagccgctaa ccctgggaag agctcgatgc gctcggaatc ccctgccatc agcccagtct
     3841 tgtcgtaagg gatgacagct cgcgttcgcg acagcgctct gaaaggaaga actgattcgc
     3901 ccagatagat aggacgtatg aattccgttt ctactactcc atcgagcggc ggtagttcct
     3961 tccaaggttt cttttcggta gcactgcgca tcgatctaac ggcttcacgc ccagcaggtg
     4021 ttccgagcgg tgacgctggt gccctttcga ccatgaacaa aacacgcgga acgatagtcg
     4081 caccttgcgc gaatcgctcg tggtacgctg aaagcgtcga tacttcccta tcaccgacat
     4141 gagacaaaat ccttatcgcg ttctcaacat cgccccatct cctactgttt ggtctgagat
     4201 cgccaatcca tcgctcgctg tcgctgggta acggcctcgg acttgatgcg cgagttccgc
     4261 gaatgactga agctgtgatc ggaaagaagt ggggccgaac tcggcgaaga tcccagggta
     4321 cctcaaatag gagatccact cgatcttccg gatccggaaa ggttcccgct cggaatccag
     4381 catactgtcc accgtttaac acactgctgg gcatcacgaa gccgaagcga ccgccccgct
     4441 tcaagtaaag ctggattgtt cgagcaacga acagcgccga caaatcctgc tgggttgccc
     4501 atttccttcc cttccagagt tcacgcgact cattcatttc cttacatgcc tgctgcatgt
     4561 cagcaggcat gtgacgaaat gcgagccatg gcggattccc gataagcaca tcgacttgat
     4621 tttcaggtcg tgcgagccac gctggccgtg ctaggttgcg gacgtagtag ccccaaatgt
     4681 gatcgcgccc ttgatcgtgc agtcggcaca tcgtcgcaaa cgttgccttg accgcttcgt
     4741 gcgagcttgc tggtatagct agtcgctgaa agatcgcctt cagcgaaggt ggcagtgttc
     4801 ccggcttgcg gtccgcggca cgatccgcca gttcctgcac gagttgatca aaaatgcgag
     4861 agttcgtgag gacttcattg ggaaagcgga gttccgaggc gagtccgggt agctcgtgag
     4921 cgtcatcggc ttggatcacg agctgcccgt gactcaagac tcgatccgtt cgcttctttc
     4981 cccattgcat cgagtcgcca agaaagacag ggatctgaat ctggcctcgc gccggatctg
     5041 ttaggcgatt acggccaagt gcgagaagat aagtcacgcg tgcgagggca accgctaccg
     5101 gatgaagatc cattcccatc acgtggtcgg ttgcaccgtc gagcaacgca gcaacgccta
     5161 gccctgcggt ctctgctgcc gcgatgtagc gcctgatagc gtgaaacaga aacgttccag
     5221 aaccacatgc tggatcaagc aaacgttcct caagcggagc tttcacggtg cgttgcacga
     5281 tctgttccgc gagccagtcc ggggtgtaat actcgccgag tttcttccga gttgcgcgac
     5341 caataatcga ttcgtagagc actttcaaga catcgtgttc aacggagtgc catgcgaagc
     5401 gactcaaacg gcgagctaag gtctttatga agttggcgcc gcctgagact tcgacaaccc
     5461 aatcaaagaa atcctgctct acaacgccat agacgccact atcctcaaat ttttcgccag
     5521 agagcagaga ggcagggctg attgcttcaa ccggcaatcc gagtacggcg tgcgcgatga
     5581 tctcggcgga gtttacaaga agcgtgtgct cgacaaacaa ttcgtccgtg tccacgaact
     5641 gggtcccgaa tgcagtcctc agtagcctcg cccacaagtg gcgctccatc acgaccgaag
     5701 acgtgttcct gttctgcgcg tagagcaccg ccagcgtagc ccggtcgagc gcatgtgcgc
     5761 tgctttcggc gccaagtctg gcctgaatcg attccggagt tggcgcgaca ttctgctttg
     5821 tcgccatcac tccttcaagc cagagcagca aaccgtccac gttcggcttt gcgctggaga
     5881 ccgcgactgt agagacctca acgagttccc catcaaccag gtgaaagcac cgccactcag
     5941 caccatccgt gagaactccc acataacgaa ggccggtagt gctctggcgc gacctgacgt
     6001 atccggccag ctgttcgacc gctttcgatc gaacgcttcc cttgcgaagg tcacgcttga
     6061 cctctatcac cgtggcccct acctcgatat cgatcttcgt gcctccgccc gcttgtgcct
     6121 ccaggaccac gtccgttaga tcgcgctctt caagatcgag cggagctttg attaggagct
     6181 gccgcaggtc cgcttgaacc tgcgcctcgg tacgaggagt gtcgcgggca gcgagcgttg
     6241 acaccaatgt gcgcagttcc tgagcgttca ttgcagcgtt tccacccgca caaggtcccg
     6301 aacggagcga cctcgttggg acgcccgtag catctgcgac cgaatgcacc gttgttgctt
     6361 catcaaggac gtacgcttgc gctaggttgc gcggaccgta accggtggat ctgacactcg
     6421 ttcccgcagc agcgatcagg gcgacatgtc ccctgtggcc ggtgcccgac cttccaaaga
     6481 cgtgtgtaga tctgggcact tatggagcaa cgagtgactc ggcgacgcgc tgtcgccgcc
     6541 tggccgccgg cggcgccatc gtccgggccg gcgaccgctg agccgttccc ctgccggcgt
     6601 accggcgccg cgacatcgtg gagatgatgt cttcgagcgc gcggtccacg gtgctacgcg
     6661 gcggtctcgc cggcctgctt cagcgagagc cgcgccttgg ccttcatcag gtcgagcagc
     6721 cggcacgcct gacggtagct ctcgagggta cggtcaggcg ggcgggaaca cggcgcgctc
     6781 gatgaagtcg gtcagcgcct ggcgggcgcg ctcggcggtg atcggcggcg ggccgcggtg
     6841 gccggcgagg ctgtaggcgg cctcgacgac ggcggagtgc aggacccagg cggtggcctc
     6901 ggggtcgggg acgacgtcgc ggccgaccag cgcggtgatc agggtggtga tccgggcgac
     6961 cgcgacctgc tcgaacgcgc gcgacacctc ggcgacctgc ggatcgcgca gcgacatctc
     7021 gcggaagctg cgggtcagct cgggctgcga cagcacgtgg tcgaacagga tcgcgatggt
     7081 ctcgctgatc gtctcgtggc gggcgcggcc gacgaagcgc tcggggccga ggtcggcgat
     7141 cgtcttgcgg tagccggcga ccatcgtgcg gcgggcgatc tccaggtaga tctcgtgctt
     7201 gtcggcgaag tagcggtaga aggtgccgac cgacaccccg gcgcgctggg cgatctccgg
     7261 agtgccgacc gcgtcgtagc cgtgggtgcc gaacagcgcg gtcgcggcgt cgatcagcgc
     7321 caggtagctg cggcgggcgc gatcctggtg aaagtcgggc tcgcgggagg ggcggagcac
     7381 caccggcagg ctgtcgagcc agagggcgtc cggttcggtg tccgggcccg tgcgagggtc
     7441 catgacgcca acaatgccct tgacgccgcc gagcgcgcta gttcaaaatt gaaacatctt
     7501 tcagtttctc cgttaggagg gttcatggaa accgccgctg ccgccacgtt cgccgcaccg
     7561 tcgatcgagg tgatcgaatc gctgctcgat gtcacgtaca cgtgggggta ccaggagact
     7621 cgccagaagc tccgcgacct ctacgacaag gcggtgcgcg cgcagtggat ctccgacgac
     7681 gtcctgccat gggacaccga cgtcgacctc ggccggccga tggggccgga gaacctgcag
     7741 ccgctgttcg gctcggacat ctacgcgcga atgaccgagg ccgagcgcca gaagctcaag
     7801 accgaggtgt tctcctggac gctgtcgcag ttcatgcacg gcgagcaggg cgcgctgctg
     7861 gcgaccgcgc agctggtcga ctcggtcgac aacatcgaca gcaagctgta cgcggcgagc
     7921 caggtcgtcg acgaggcgcg ccacgtcgac gtctacaacc gctacgtgca cagcaagatc
     7981 gggttctcgt acccgatcaa cccgcacctg cggacgctgc tcgacctgat cctcaaggac
     8041 agccggtggg acatgaagtt cctcggcatg cagatcatgg tcgaggggct cgcgctcgcc
     8101 gcgttcggga tgat
//
LOCUS       DASVOR010000064         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_234411,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000064 DASVOR010000000
VERSION     DASVOR010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234411"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="VF516_01440"
     CDS             <1..345
                     /locus_tag="VF516_01440"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8106352.1"
                     /translation="FPRLDERRRQLAGSLSGGEQQMCAIARALLSEPSLLMVDELSLG
                     LAPGVVDQLMRAVGELHRGGMTILLVEQNLQKALAVAERGVIIETGRLRLAGTSAELA
                     GNPEIRAAYLGM"
     gene            408..1451
                     /locus_tag="VF516_01445"
     CDS             408..1451
                     /locus_tag="VF516_01445"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8106353.1"
                     /translation="MTTTNRRIVLASRPTGAPREDNFRLETVPIPDLAPGQVLVRHHY
                     LSLDPYMRGRMSDAKSYAAPQPLDEVMIGGTVGEVVATRSERFAVGDQVLGMGGWQDH
                     AVVAADGESSTGSQRGAAIRKVDSSRIPLPAYLGAVGMPGVTAWYGLNMICEPKAGET
                     VAVSAASGAVGGVVGQLAKAKGCRVVGIAGGKDKCDYVVGELGFDACVDYKAAATPGS
                     LQAMVRDATPSGIDGYFENVGGAVLDAVMLQMNPFGRIAVCGMIAGYNDTPTPMALPR
                     LILTQRLKIQGFIVSEHPALWPKALAELGAGVATGAIKYRESVAQGLEAAPAAFLGML
                     QGKNFGKQLVKLV"
     gene            1465..1989
                     /locus_tag="VF516_01450"
     CDS             1465..1989
                     /locus_tag="VF516_01450"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8106354.1"
                     /translation="MARIPYPDPKTLLPETQDQLAKLPPLNLFRMLAGGEGLLQAFVR
                     FGNHLWFKADLPPVLREIAILRVGALSNARYEVFQHERIARGLGMSEDLIAAIRRGPD
                     DPAFDELQRLVVRFTDDLVRNIRASDDTFQPLAERMPVRQLQELTVVVGYYMTVSRFL
                     ETFGIEIEGQGSPG"
     gene            complement(2038..3027)
                     /locus_tag="VF516_01455"
     CDS             complement(2038..3027)
                     /locus_tag="VF516_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106355.1"
                     /translation="MQLDVVIFWVTHALRGSLQPVMRNRFDQLAKQIGKTALEGSGLT
                     VVHAEITPEPLHADLLHEPDPAKATERKRLGLLGRLVSLPCLLEPYSDAPSAEEFRAC
                     LTKHLAWWQQQARQHRADGKKSAEPRRKPENPFLWILCGGAPDSILTKLTLKATSEWP
                     AGVYLFGDDVLRVGIVVARELPRERTTLLIRLMASAGPTLNEAIADLHTLPLNAYERS
                     VAERPLLHCRHMIRNRPTLTPEEQEFVETMDKTWEDSRQEGHAEGLAEGRAEGRLETL
                     AESVLAVLRVRGIAVPAAARKRILAQKDLTRLQRWHEQAVVVTSVGDLFKSSR"
     gene            3284..4048
                     /locus_tag="VF516_01460"
     CDS             3284..4048
                     /locus_tag="VF516_01460"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX8106356.1"
                     /translation="MSVLSDLVTAIASGAVEVIDLTAPLSAQTPILKLPEPLGQTWRF
                     GLQEISRYNEAGPAWYWNNITTGEHTGTHLDAPIHWISGKDGADVSQLPARRLIAPAA
                     VIDFSAEATSNPDFLLEIKHIDAWQKQHGPLPKGGWLLFRTGWAARSQDEDRFLNAGH
                     TPGISVECAKWLAETAPVLGVGVETVGTDAGAAHSFDPAFPCHSFLLGHDKYGLTQLQ
                     NLARLPPTGAVVFVGPLPIVRGSGSPCRALALVERT"
     gene            4045..>4484
                     /locus_tag="VF516_01465"
     CDS             4045..>4484
                     /locus_tag="VF516_01465"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8106357.1"
                     /translation="MKVAEAVGRGLVAAGIDHVFGVVGSGNFHVTNAMVAAGARFLPA
                     AHEGGAASMADGYARVSGRLTALSVHQGPGVTNALTGIVEAAKSRTPLVVLAPEATQP
                     RSNFFVDLPGIAAAIGAGFRRVTAERVASDVAEACRAAVSGAGQT"
BASE COUNT          655 a         1528 c         1605 g          696 t
ORIGIN      
        1 ttcccgcggc tcgacgagcg ccgccgccag ctcgccggca gcctgtcggg cggcgagcag
       61 cagatgtgcg cgatcgcccg cgcgctgttg agcgagccgt cgctgctgat ggtcgacgag
      121 ctcagcctcg gcctggcgcc gggcgtggtc gaccagctga tgcgcgcggt cggcgagctg
      181 caccgcggcg gcatgaccat cctgctggtc gagcagaacc tccagaaggc gctggcggtc
      241 gccgagcgcg gcgtgatcat cgagaccggc cggctgcggc tcgccggcac cagcgccgag
      301 ctcgccggca atccggagat ccgcgccgca tatctcggga tgtgagggcg cgccgaccga
      361 cggaccggcg ggcgggttag gctgcgtgcg aagctcgcag gagcgccatg accaccacga
      421 atcgccgcat tgtccttgcg tcgcgcccga ccggcgcgcc gcgcgaggac aacttccgcc
      481 tcgagaccgt cccgatcccg gacctcgcgc ccggccaggt gctggtgcgc catcactacc
      541 tgtcgctcga tccgtacatg cgcggccgga tgagcgacgc gaagtcgtac gccgcgccgc
      601 agccgctcga cgaggtcatg atcggcggca cggtcggcga ggtcgtggcg acccgctcgg
      661 agcggttcgc ggtcggcgat caggtgctcg gcatgggcgg ctggcaggac cacgccgtgg
      721 tggctgcgga cggcgagtcc tcgacggggt cccagcgggg cgcggcgatc cgcaaggtcg
      781 acagctcgcg gatcccgctg cccgcgtacc tcggcgcggt gggcatgccc ggcgtcaccg
      841 cgtggtacgg cctgaacatg atctgcgagc ccaaggccgg cgagaccgtc gcggtcagcg
      901 cggcctcggg cgcggtcggc ggcgtggtcg gccagctcgc caaggccaag ggctgccgcg
      961 tggtcggcat cgccggcggc aaggacaagt gcgactacgt cgttggcgag ctcgggttcg
     1021 acgcgtgcgt cgactacaag gccgctgcca cccccgggtc gctccaggcg atggtgcgcg
     1081 acgcgacccc gagcggcatc gacggatact tcgagaatgt cggcggcgcg gtgctcgacg
     1141 ccgtgatgct gcagatgaac ccgttcggcc ggatcgcggt gtgcggcatg atcgccggct
     1201 acaacgacac gccgaccccg atggcgctgc cccggctgat cctgacccag cgcctgaaga
     1261 tccagggctt catcgtgtcg gagcatccgg cgctgtggcc caaggcgctc gccgagctcg
     1321 gcgccggggt cgcgaccggg gcgatcaagt accgcgagtc ggtggcccag ggcctggaag
     1381 ccgcgccggc cgcgttcctc ggcatgctcc agggcaagaa cttcggcaag cagctggtca
     1441 agctggtgtg acaggagggt aacaatggca cgtattccgt atccggatcc caagacgctg
     1501 ttgcccgaga cccaggatca gctcgccaag ctgccgccgc tcaatctgtt ccgcatgctg
     1561 gcgggcggcg agggcctgct tcaggcgttc gtccggttcg gcaaccacct gtggttcaag
     1621 gccgatctgc ccccggtgct gcgcgagatc gcgatcctgc gggtcggcgc gctgtccaac
     1681 gcgcgctacg aggtcttcca acacgagcgg atcgcgcgcg gcctcgggat gtcggaggac
     1741 ctgatcgccg cgatccgccg cggccccgac gacccggcgt tcgacgagct ccagcgcctg
     1801 gtcgtgcggt tcaccgacga tctcgtgcgc aatatccggg cctcggacga cacgttccag
     1861 ccgctcgccg agcgcatgcc ggtgcgccag ctccaggagc tgaccgtcgt ggtcggctac
     1921 tacatgaccg tgagccggtt cctcgagacc ttcgggatcg agatcgaggg ccagggctca
     1981 ccgggctgac gcgcgcctgc gggtacgcag cggccgacgc tgccgtcgac ggcggcgtca
     2041 gcggctgctc ttgaaaaggt cgccgacgga tgtcacgacg actgcctgct catgccagcg
     2101 ctgcagccgc gtgagatcct tctgcgcgag gatgcgcttg cgcgcggcgg ccggaaccgc
     2161 gatgccccgg acccgtagca cggcgagaac ggactcagcg agcgtttcca gtcgtccctc
     2221 cgcgcgtccc tccgcgagtc cttccgcgtg tccttcctgg cgcgaatctt cccaggtctt
     2281 gtccatggtc tccacgaact cctgttcttc gggggtcaag gtaggccgat tccggatcat
     2341 atgcctgcag tgtagcagcg ggcgctcggc gacggaacgc tcatatgcat tcagcggtag
     2401 cgtgtgaaga tctgcgatcg cctcgttcaa tgtgggcccg gctgacgcca tgaggcggat
     2461 cagcagcgtc gtgcgttcac gtggcagctc tctcgcgacg acgatgccga cgcgaagtac
     2521 gtcatcgccg aacaaataca caccagctgg ccactcagat gtagctttga gcgtcagttt
     2581 ggtcaggatg ctgtccgggg caccaccgca gaggatccag aggaagggat tctcgggctt
     2641 gcgccgtggc tctgccgatt tcttgccatc agctctgtgc tggcgcgcct gctgctgcca
     2701 ccacgcgagg tgcttggtca gacaggcgcg aaactcctcg gcgctgggtg catcactgta
     2761 gggttccagc aggcagggca gcgataccag ccggccgagg aggccgagac gcttgcgctc
     2821 ggtggccttc gccggatcgg gttcgtggag aagatccgcg tgcagcggct ccggggtgat
     2881 ctcggcgtgg acgaccgtga ggcccgaacc ctcgagcgcc gtcttgccga tctgcttggc
     2941 cagctggtcg aagcgattgc gcatgacggg ctgcagactg ccacggagag cgtgagtgac
     3001 ccagaaaatg accacgtcga gctgcatatt tcggatacgg agtgagcatt gagccttcga
     3061 tgatgccgat gctctcgggt tctggatgtc cggataccgg acgaatgtca tggggggcga
     3121 tacggcgcat gccggatgtg ctcgggcccg gggatcgagg cggcaggtaa cccggggcgc
     3181 ccaggccggc cgtgctcccc cgggacggtc gggggccgga ccggcgggcg atcgccggcg
     3241 caagtgcatt gattcgcggc gaccacttgc gtagcctcgc ggcatgagcg tgctctccga
     3301 tctggtcacc gccatcgcat cgggcgccgt cgaggtcatc gacctgaccg cgccgctctc
     3361 ggcacagacg ccgatcctca agctgcccga gccgctcggg cagacctggc gattcggcct
     3421 gcaggagatc agccggtaca acgaggccgg tccggcctgg tactggaaca acatcacgac
     3481 cggcgagcac accggcacgc acctcgacgc gccgatccac tggatcagcg gcaaggacgg
     3541 cgccgacgtg tcgcagctgc cggcgcgccg gctgatcgcg cccgcggcgg tgatcgactt
     3601 ctcggccgag gcgacgtcca atccggactt cctgctcgag atcaagcata tcgacgcgtg
     3661 gcagaagcag cacgggccgc tgcccaaggg cggctggctc ctgttccgca ccggctgggc
     3721 ggcgcgctcg caggacgagg accggtttct caacgccggt cacacgccgg ggatctcggt
     3781 ggagtgcgcg aagtggctcg ccgagaccgc gccggtgctg ggcgtcggcg tcgagacggt
     3841 cggcaccgac gccggcgccg cgcactcgtt cgatccggcg ttcccgtgcc actcgttcct
     3901 gctcggccac gacaagtacg ggctgaccca gctgcagaac ctggcgcggc tgcccccgac
     3961 tggcgcggtc gtgttcgtcg ggccgctgcc gatcgtgcgc ggctcgggca gcccgtgccg
     4021 cgcgctcgcg ctcgtcgagc ggacatgaag gtcgccgagg cggtcggccg cggtctggtc
     4081 gcggccggca tcgaccacgt gttcggcgtg gtcggcagtg gcaactttca tgtcaccaac
     4141 gcgatggtcg cggccggcgc gcggttcctc cccgccgcgc acgagggcgg cgccgccagc
     4201 atggccgacg gttatgcccg ggtgtcgggc cggttgaccg cgctgtcggt gcaccagggc
     4261 cccggcgtga ccaacgcgct caccgggatc gtcgaggccg ccaagagccg cacaccgctg
     4321 gtggtgctcg cgcccgaggc gacccagccg cggtcgaact tcttcgtcga tctgccgggc
     4381 atcgcggcgg cgatcggcgc cgggttccgc cgggtcaccg ccgagcgggt ggcgagcgac
     4441 gtcgccgagg cctgccgggc ggcggtgtcc ggcgcgggcc agac
//
LOCUS       DASVOR010000065         3879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_146789,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000065 DASVOR010000000
VERSION     DASVOR010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146789"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VF516_01470"
     CDS             <1..493
                     /locus_tag="VF516_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106358.1"
                     /translation="EDFRIVYVSIQRTHIHLIVEADDKAALAVGMQGFQISAAKHLNA
                     AISKGKPGPRRRGTVFPDRYHAVIITSPRQARHALSYVLSNFRRHEEDRVAPMSGWTV
                     DWFSSGIVFPGWAEYGDEPFLWRGPPTYDPLIVYQPRTWLLREGWKKAGEISCREVPS
                     ARQ"
     gene            539..970
                     /locus_tag="VF516_01475"
     CDS             539..970
                     /locus_tag="VF516_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106359.1"
                     /translation="MGRLGWAGSDGRIGIEPRQSIMRSTSQEAATLLDILVDGFEKNE
                     AFGGFHRRELPMPDESAAARTFDALAEEAQRWKGPPERRQEDAMRRLAAWPDLEIRRA
                     GRGVMVLARAPGFAWWHDERTWEGNPMGAVRAWLREDRGST"
     gene            1020..2063
                     /locus_tag="VF516_01480"
     CDS             1020..2063
                     /locus_tag="VF516_01480"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8106360.1"
                     /translation="MIEAGETEWCGDQRIYVIDRTAAGFAYGVPVAEMRRSAERSAGG
                     AGWARAKRILRELLEREVGEVDDIGWVKKIGDGLSREIFVSEVELVGGRCEAYVVALP
                     RADAEPGLDDRTRRELRLIARLRTRSFPFRLPEMVGAFPDDDRLALVRRFVPGVELEL
                     RSGRQPVKPWAIVGEIAAAVHAVPGTDVADIVPGAATRREHALEAISALAGLGPVEMR
                     DAHAWALEHLPPAEPSALLHSDLLGQNILLSVDGSHHVIDWEYARTGDPAYDLAIVTR
                     GVRQPFQIGRGLERLLEAYRDHGGREIAAEHVRVHELGLIAGWYREAVSGARAHSPQH
                     ELDQMRSLLRRLR"
     gene            2593..3699
                     /locus_tag="VF516_01485"
     CDS             2593..3699
                     /locus_tag="VF516_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106361.1"
                     /translation="MKQVVAPSSSREPGTLQYGVLHSQGTFFDVPISSAPVAVRSDGS
                     VVGATCSGVVAFDATLHETGRVDLDSRSNAFAVNVAVAPDDAVYALVPEGMIRIDDLD
                     HVSTAREARWITPVGATLGVLVAGTEGPYTEPDSDFGVPPQNFTIHGFDALTGQPRTV
                     ASGQYLLAAARGGGVFTVEQQGKQSATLRRLDPDGTAVWSRTLTSTFDGLVIQGAAAA
                     ADGGVIVFGDSPTPVDFGDRTLASPGQFVAGFDVSGMTRWGFTSASFITHIAQTAQGE
                     ILIAGDTALGGGEGAFSTDASLSVATQAGISRTLYIRGPGDQRITGLAATPDGFAWIA
                     VTNFRHDDPEPEPVIQIGAHTFPDSGGYLFKIVP"
BASE COUNT          578 a         1198 c         1494 g          609 t
ORIGIN      
        1 cgaggacttc cggatcgtgt acgtcagcat ccagcgcacg cacatccact tgatcgtcga
       61 ggccgacgac aaggctgccc tggcggtggg gatgcaaggc tttcagatct ccgccgccaa
      121 gcacctcaac gcggcgatca gcaagggcaa gccggggccg cgccggcggg gcacggtgtt
      181 tcccgaccgc tatcatgccg tgatcatcac gtcgccgcgg caggcacggc acgcgctgtc
      241 ctacgtgctc tccaacttcc ggcggcacga ggaagatcgc gtggcgccga tgtcggggtg
      301 gacggtcgac tggttctcga gcgggatcgt gttccccgga tgggcggagt acggagacga
      361 gccgttcctg tggcgtggcc cgccgaccta tgatccgctg atcgtgtacc agcccaggac
      421 ctggctgctg cgagaaggct ggaagaaggc cggagagatc tcctgtcgcg aggttccgtc
      481 ggcgaggcag tgagcatggc tgccagcgca tgaagcgcga ggtagagtcg cggctcgaat
      541 gggccggctc ggatgggccg gctcggatgg ccggattggc atcgagccga ggcagagcat
      601 catgagatcc acatcgcagg aagccgcgac gctgctggat atcctggtcg acggcttcga
      661 gaagaacgaa gcctttggcg ggtttcatcg acgggagctg ccgatgccgg acgagagcgc
      721 cgcggcgcga acgttcgatg cgctggcgga ggaggcgcag cgctggaagg gtccacccga
      781 gcggcggcag gaagacgcga tgcgacggct tgcggcgtgg cccgatctgg agattcgccg
      841 ggccggacgt ggcgtgatgg tgctggctcg cgcgccaggg ttcgcgtggt ggcacgacga
      901 gaggacgtgg gagggcaacc cgatgggggc ggtgcgcgcc tggctccgcg aagatcgtgg
      961 ctcgacgtag gcgccgtggc cggcggatgc cggtggcctg gagccctcat gacgagccgg
     1021 tgatcgaggc tggtgagacc gagtggtgcg gcgatcagcg gatctacgtg atcgatcgca
     1081 ccgccgccgg cttcgcctat ggggtgccgg tcgccgagat gcgccgcagc gccgagcgct
     1141 ctgcgggtgg tgccggctgg gcgcgcgcca agcggattct gcgagagttg ctggagcgcg
     1201 aggtcggcga ggtcgatgac atcggctggg tcaagaagat cggtgacgga ctctcgcgcg
     1261 agatcttcgt gtccgaggtc gagctcgtcg gcgggcgttg cgaggcgtac gtggtcgcat
     1321 tgccgcgcgc cgatgcggag ccggggctcg atgaccgcac gaggcgcgag ctgcgactga
     1381 tcgcgaggct gcggacgcgg agctttccgt tccggctccc cgagatggtc ggagcgttcc
     1441 ccgatgacga ccggctcgcg ctggtgcggc ggtttgttcc gggggtcgag ctcgagctgc
     1501 gcagcggccg gcagccggtg aagccgtggg caatcgtcgg cgagatcgcg gccgccgttc
     1561 atgcggtgcc cggaaccgac gtcgcggaca tcgtgcccgg agccgcgacg cggcgcgagc
     1621 acgcgctcga ggcgatctcg gcgctggccg ggctcggccc ggtcgagatg cgcgatgcac
     1681 acgcgtgggc gctcgagcac cttccaccgg cggagccgag cgcgctgttg cacagcgatc
     1741 tgctcgggca gaacatcctg ctgtcggtcg acggttcgca tcacgtgatc gactgggagt
     1801 acgcgcggac gggggatccg gcgtacgacc tggcgatcgt cacgcggggc gtccggcagc
     1861 ccttccagat cggtcgtggg ctcgagcgct tgctcgaggc gtaccgcgat cacggcggtc
     1921 gcgagatcgc cgccgagcac gttcgtgtgc acgagctggg cttgatcgcc gggtggtacc
     1981 gcgaggcggt gtccggcgcg agggcgcatt cgccgcagca cgagctggac cagatgcgga
     2041 gcctgctgcg acggctgcgc tgaccgccgg gcggtgacgg cggccggcgg cgtagacgca
     2101 caggaaggcg gcgacgagca gcgtggcaat gctccgcgcg atcctcatgg gacacggtag
     2161 gctcaccggt ctgctcgccc agcaggggta ctgcgtacgg gggcaaaccg gctgcggtcg
     2221 ctggagctgt ggcagcgcga cggcgaggcg atggagcagc gcgaggatcg gcgagagcgc
     2281 gtgcttgatc gtggcaggct gcagcgcgaa tgggcggtcg agcgttcgct gccgcgtcga
     2341 ccgaccaggc tgccggccgg tggcaacccg acgcgtgaga ccatgtcgcc gtggccctgg
     2401 catcgctgtc atggcgaggc ggggtgcgga tccgcggcgg gtcggccatg ggcagtagag
     2461 cgatgaaatc cttggggcag cgcgcggcat cctgtgtgca ttttgcagcg ggccatgcgc
     2521 cgagtcatga cgtgcacggg agcgctggtt ctcgcagcgg cgtgcagtgg accagacacc
     2581 ccgccctgtg gcgtgaagca ggtcgtcgcg ccgtcgtcga gccgcgaacc gggaacgctg
     2641 cagtacggcg tgctgcattc gcagggaacc ttcttcgatg tgcccatcag cagcgcaccg
     2701 gtcgcggtgc gctccgacgg cagcgtggtg ggcgcgacgt gcagcggcgt tgttgcgttc
     2761 gatgcgacgc tgcacgagac cggtcgggtc gacctcgact cgcggagcaa cgcgttcgcg
     2821 gtgaacgtcg cggtcgcgcc ggatgacgcg gtgtacgcgt tggttcctga aggcatgatc
     2881 cggatcgatg atctcgatca cgtgtccacg gcccgtgagg cgcgctggat cacgccggtc
     2941 ggggcgacgc tgggcgtcct cgtggcgggg acggaggggc cctacaccga accggactct
     3001 gatttcggag tgccaccgca gaacttcacg atccacgggt tcgatgccct gaccggccag
     3061 cctcgcacgg ttgccagtgg gcagtatctg ctcgccgctg cccgcggcgg cggcgtgttc
     3121 accgtcgaac agcagggcaa gcaatccgcg acgctgcgcc ggctggatcc ggacggcacg
     3181 gcggtgtggt cgcgcacgct cacctcgacg ttcgacggcc tcgtgatcca gggagcagcg
     3241 gcggccgcgg acggcggcgt gatcgtgttc ggagattcgc ccaccccggt ggacttcggc
     3301 gatcgcacgc tcgcctctcc aggccagttc gtcgccggat tcgacgtctc gggcatgact
     3361 cggtgggggt tcacatcggc gtccttcatc acgcacatcg cacagaccgc gcagggcgag
     3421 atcttgatcg cgggcgacac cgcccttggt ggtggcgagg gggctttcag cacggatgcc
     3481 tccctctcgg ttgcgacgca agccgggatc tcgcgtacgc tctacatcag ggggccagga
     3541 gatcagcgta tcaccgggct ggcggcgaca ccggatggct tcgcgtggat cgccgtcact
     3601 aacttcaggc acgatgatcc ggaaccggag ccggtgatcc agattggcgc tcacacgttc
     3661 ccggactccg ggggttatct gttcaagatc gtgccctgag aaggcatccg gccgtcaggt
     3721 cagcggccag cgcagccacc cgagcaggcg cgggaggccg ccgaggccgg tgcgggcggc
     3781 gcggtaggcg gtgtcggccg aggcctggat ctcggcgagg tcgctggcgt cgaggccgac
     3841 gccgtggcgg gcgcgctcga ggatggtggc gcagtggtc
//
LOCUS       DASVOR010000066         5369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_380565,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000066 DASVOR010000000
VERSION     DASVOR010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5369
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380565"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            692..1084
                     /locus_tag="VF516_01490"
     CDS             692..1084
                     /locus_tag="VF516_01490"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II TA system antitoxin MqsA family protein"
                     /protein_id="HEX8106362.1"
                     /translation="MRCPNCNKDALHDWEGHITRMGIKISARGRRCSVCGETLYDHEE
                     VGRQEQELAAALVDRGIRTGLEFALVRKAAGFRAVEIAELFGVRPETVSRWEHGESEI
                     PRTAAFALSQLYTHPRLTRQSFEALATT"
     gene            complement(1239..1694)
                     /locus_tag="VF516_01495"
     CDS             complement(1239..1694)
                     /locus_tag="VF516_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106363.1"
                     /translation="MAMPFSEKQGRPSGFFSEVLTSLLTPAGVQAGFTVETAKREGSD
                     VIHSTIINDLIDADLVIADLTDHNQNVLFELGVRMALEKPVALIKAKGTGPIFDVDGT
                     LRVKEYDPNLWPSTIEADIPNLSAHFLGAWENPNNSPTYMSLLRAKPKT"
     gene            complement(1663..2235)
                     /locus_tag="VF516_01500"
     CDS             complement(1663..2235)
                     /locus_tag="VF516_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106364.1"
                     /translation="MSQTDTEGPDDVAIEKPAKPTRKAADDDRKGPARKKATKPSKRT
                     TKGAGSRGPQSKRPQHEFPVVSLEVALKIPQKIKEINQGNPWDSNDVAKASVNTTKTN
                     NKFFYTATASRDYGLTVGGRDSDKVSLEELGQDIVYATTPETKHLSKLRAFFNIPLFE
                     KVFTFTKEIISPGQDDDQSLCGDAFLGKTR"
     gene            complement(2302..3225)
                     /locus_tag="VF516_01505"
     CDS             complement(2302..3225)
                     /locus_tag="VF516_01505"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8106365.1"
                     /translation="MKLQDRMDPAQLARLGSLPIQARVIVEGALSGLHRAAVHGSSVE
                     FAEHKEYSPGDELRHVDWKAYAKLDRYYVKQFEQESQLTVYLVLDASASMAFSGGGIA
                     KLEYGGLLLAALAYLVIQQQDKVGLLASGDRSLETMVPPRGRTTHFHDLMAVLESILA
                     RGAAGDESPAAALARIAELTRRRRALIILASDLFDPDDETLRALAQLRAQRHDVSVLH
                     LLDPHERSFPYDGLTQFDALESAHKLLVNPAAIRRDYLDRMEAFLTKVRGALTAAGVD
                     YHLVDTSQPLEQTLLTLLVTRSRLRPARRAG"
     gene            complement(3238..4356)
                     /locus_tag="VF516_01510"
     CDS             complement(3238..4356)
                     /locus_tag="VF516_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8106366.1"
                     /translation="MNKSRPTPVTGEKLADITVLRGDPGDGPDADPEMLPLAEESTVV
                     IKNDLDAVAELAQAHDEIMAEINKRIIGQRRVIEQLLVALFARGHTLFVGVPGLAKTL
                     LISTLAETLNLSFQRIQFTPDLMPADITGTEVLEEDHTTGRRIFRFIKGPVFANLVLA
                     DEINRTPPKTQAALLQSMQEYRVTAGGTTHELPLPFLVFATQNPVEQEGTYPLPEAQL
                     DRFMFQVDVDYPSEAEEEEIVRQQTSDHRPELMRVLSPQRIVQLQDLVRRVPAADHAV
                     KYAVALARATRPSSPNAPPFIREAVSWGAGPRASQFLILAAKARAILGGRFAVSIEDV
                     AALARPILQHRLILNYRAEAEGLRAGDLVDRLLATVTP"
     gene            complement(4356..>5369)
                     /locus_tag="VF516_01515"
     CDS             complement(4356..>5369)
                     /locus_tag="VF516_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106367.1"
                     /translation="ETDIETTGTIALSKYNKLMVVSPRATIFGYPWMDTLAHEYTHLV
                     VSRVAHDAVPVWLQEGLARLEQTRWRKGPELQLSTTEQALLSAALRKGRLITFEEMHP
                     SMAKLPSQEAAALAYAEVYTLVGWMQSKIGYRGIRDALVSQRDGKSAKRAVAEALGIP
                     WPAVEKEWRAHLKGGDSKARAGKLIRFAKGGVDSENVGLEQVSARARKHARLGGMLRA
                     RGQNEAAVVEYEKALTGGPEPFVAGKLARTLVEIGRFDRAIELATPLVTADDHDAVAA
                     VTLGMARSARHQWREAITAYEQALGVSPFDPTTRCGLAEAYAQTHDPRAARERSACDQ
                     LRN"
BASE COUNT          828 a         1662 c         1882 g          997 t
ORIGIN      
        1 cccacgatga cctggtgaag ctcgccgcag tcctcgcttc cgcaccccga gagggcccag
       61 gccaccgcgc acgcgatgac tcgccacatg gcggacctgg gctgcaggcg caatgccaag
      121 gcccccaggc gctcttcgca gcgatttcat cgggctgccc cgtctccggc ccgactttgt
      181 ctcgcgaacc gcgcgacacc atgacagcac cgccatggtc acggccgaag gaacggccgc
      241 tcgccgcggt cctccgcgca caggcccccc ggtcacgctc ccgctgcgtg cgatcccggg
      301 tcgtgcgttc tccggccaac ctcgacctgt tactctgcgc acttctttct aacctggtga
      361 cttcgctagc ctcgtcgagc ttccttgaca ttttcgcaag tcggttcatc ctggagcatg
      421 gcgtgaacga cacgaaggcc ctctcggaga tccagcggct ggcaagtttg aaccggatcg
      481 tctacaccgg tcacgcactt ctccgcatgg atagccgagg agcgagccgg gatgacgtcc
      541 gcagcgcctt gatcaccgcg acctcggccc gccggcaaga gcgtggtacc tggcgcgttg
      601 agggcgggcg ggaccttgat ggcgatgatg acggcaatcg tcgatatcga agccgacgtg
      661 atcgtcgtga ccctgttctg aggaggccta catgcgatgc ccgaactgca acaaggatgc
      721 gctccacgac tgggagggtc acatcacgcg gatgggaatc aagatctccg cgcgtgggag
      781 gcggtgttcg gtctgtggtg agaccctgta cgaccacgag gaggtcggtc gccaggaaca
      841 ggagctcgcg gccgcgcttg tggatcgcgg catccgcacc ggtctggagt tcgcgctcgt
      901 gcgcaaggcg gcggggtttc gcgccgtcga gatcgccgag ctgttcggcg tgcggccgga
      961 gaccgtctcg cgctgggagc acggcgagag cgagatccct cgcaccgcgg cgttcgcgct
     1021 cagtcagctg tacacgcatc ccaggctgac gcggcaaagc ttcgaggcgc ttgccacaac
     1081 ctgagtgagc cgcggcagga aggttagtgc ggctgccgct tcggttaacc ggtgccggag
     1141 gcggtgcgct tcagtgacca ctgccgctgc gtcgtgttgg cgtcgcattc ggcggattct
     1201 agtgtggcat tagcattgct agccatgccc atggtaggtc aagtcttcgg tttcgctctt
     1261 agaaggctca tgtaagttgg agaattgttt ggattctccc atgcgcctag aaaatgagct
     1321 gaaaggttgg ggatatcagc ttcaattgtt gaaggccaca agttcggatc gtattctttt
     1381 acgcgaagcg tgccatctac gtcaaagatc ggtccagttc ctttagcctt gattaaggca
     1441 acaggcttct cgagagccat tcgaacgccc aattcgaata gtacattttg attgtggtcc
     1501 gtgagatcag caataacgag atctgcatct atgagatcat taatgatggt cgagtgaatg
     1561 acatcactgc cttcgcgctt ggctgtttca acggtaaatc cggcctggac ccctgcaggt
     1621 gtcagcaagc tcgtaaggac ttcggaaaag aacccactcg gtctaccttg tttttccgag
     1681 aaaggcatcg ccacaaaggc tttgatcgtc gtcttgccct ggggagatta tttcctttgt
     1741 aaaagtgaaa actttctcaa agagcggaat attaaagaac gctcggagct tagacagatg
     1801 cttcgtctca ggggttgttg catagacaat gtcttggccg agttcctcga gagaaacttt
     1861 gtccgagtcg cggcccccaa cggtaagccc gtagtctctt gaggcggttg ctgtgtagaa
     1921 gaacttgtta ttggtcttcg tcgtgttgac agacgccttg gcgacatcgt tcgagtccca
     1981 cggattacct tggttgatct ccttgatttt ttgagggatc ttgagcgcga cctcaagact
     2041 aacgaccggg aattcgtgct gcggtcgttt gctttgcggc ccgcgagagc cggctccttt
     2101 ggtagtccgt ttacttggct tcgtcgcctt cttccgcgca gggcctttgc ggtcgtcgtc
     2161 cgcagccttc cgtgtcggct tcgcaggctt ctcgatcgcc acatcatctg ggccctcggt
     2221 gtctgtctgg ctcatggatc gcctttgttg agcgatcatc tcaacatgta gacctcgacc
     2281 ttcgctccgt cccttcccct ttcaacccgc ccggcgggcg gggcggaggc gggagcgggt
     2341 gaccagcagg gtgagcaggg tctgctcgag cggctggctg gtgtcgacca ggtggtagtc
     2401 gacgccggcg gcggtcagcg cgccgcggac cttggtgagg aaggcctcca tgcgatcgag
     2461 gtagtcgcgg cggatcgcgg ccgggttgac cagcagcttg tgggcgctct cgagggcgtc
     2521 gaactgggtc aggccgtcgt aggggaagct gcgctcgtgg ggatcgagca ggtggaggac
     2581 cgagacgtcg tggcgctggg cgcggagctg ggcgagcgcg cgcagggtct cgtcgtcggg
     2641 atcgaacagg tcggaggcca ggatgatcag ggcgcggcgg cggcgggtga gctcggcgat
     2701 ccgggcgagc gcggcggcgg gggattcgtc gccggcggcg ccgcgcgcga ggatcgactc
     2761 gagcaccgcc atcaggtcgt ggaagtgggt ggtgcggccg cgcggcggga ccatggtctc
     2821 gagcgagcgg tcgccggacg cgagcaggcc gaccttgtcc tgctgctgga tcacgaggta
     2881 ggcgagggcg gcgagcagga ggccgccgta ctcgagcttg gcgatgccgc cgccggagaa
     2941 cgccatcgag gccgaggcgt cgagcacgag gtagacggtg agctgcgact cctgctcgaa
     3001 ctgcttgacg tagtagcggt cgagcttggc gtaggccttc cagtcgacgt ggcgcagctc
     3061 gtcgccgggc gagtactcct tgtgctcggc gaactcgacc gacgagccgt gcacggccgc
     3121 gcggtgcagg ccggacagcg cgccctcgac gatgacccgg gcctggatcg gcaggctgcc
     3181 gagccgcgcg agctgcgcgg ggtccatgcg gtcctggagc ttcacggggt cacggggtca
     3241 cggggtcacg gtggcgagca ggcggtcgac caggtctccc gcgcggagcc cttccgcctc
     3301 ggcacggtag ttgaggatca ggcggtgctg caggatcggc cgggcgagcg cggcgacgtc
     3361 ctcgatcgag accgcgaacc ggccgccgag gatcgcgcgg gccttggcgg cgaggatcag
     3421 gaactgcgac gcgcgcgggc cggcgcccca cgacacggcc tcgcggatga acggcggggc
     3481 gttgggcgag ctcggccggg tcgcgcgggc gagcgcgacg gcgtacttga cggcatgatc
     3541 ggcggccggg acgcggcgga ccaggtcctg gagctggacg atgcgctgcg gcgacagcac
     3601 gcgcatcagc tcggggcggt gatcgctggt ctgctggcgg acgatctcct cctcctcggc
     3661 ctcgctcggg tagtcgacgt cgacctggaa catgaagcgg tcgagctggg cctcgggcaa
     3721 cggataggtg ccctcctgct cgaccgggtt ctgggtcgcg aacaccagga acggcagcgg
     3781 cagctcgtgg gtggtgccgc cggccgtgac ccggtactcc tgcatcgact ggagcagcgc
     3841 ggcctgggtc ttgggcgggg tgcggttgat ctcgtcggcg agcaccaggt tggcgaacac
     3901 cgggcccttg atgaaccgga agatccggcg gccggtggtg tggtcctcct cgagcacctc
     3961 ggtgccggtg atgtcggccg gcatgagatc gggagtgaac tggatgcgct ggaacgacag
     4021 gttgagggtc tcggcgaggg tcgagatcag gagcgtcttg gccaggccgg ggacgccgac
     4081 gaacagggtg tggccgcgcg cgaacagcgc gaccaggagc tgctcgatca cccggcgctg
     4141 gccgatgatc cgcttgttga tctcggccat gatctcgtcg tgggcctggg cgagctcggc
     4201 gaccgcgtcg agatcgttct tgatgacgac ggtcgactcc tcggcgagcg gcagcatctc
     4261 gggatcggcg tccggaccgt cgcccggatc gccgcgcagg acggtgatgt cggcgagctt
     4321 ctcaccggtg accggagtgg ggcggctctt gttcatcagt ttcttagctg gtcacacgcg
     4381 gagcgctcgc gcgctgcgcg ggggtcgtgg gtctgagcat acgcctcggc caggccgcag
     4441 cgcgtggttg gatcgaacgg gctcacgccg agggcttgtt cgtacgcggt gatcgcctcg
     4501 cgccactggt ggcgggcgga gcgtgccatg cccagggtca ccgccgccac cgcgtcgtgg
     4561 tcgtccgcgg tgaccagcgg cgtggcgagc tcgatcgcgc ggtcgaaccg cccgatctcg
     4621 accagcgtgc gcgccagctt gccggcgacg aacggctccg gaccgccggt cagggccttc
     4681 tcgtactcga ccaccgcggc ctcgttctgg ccgcgcgcgc gcagcatgcc accgagccgg
     4741 gcgtgcttgc gtgcgcgggc gctgacctgc tcgagcccga cattctccga gtcgacccca
     4801 cccttggcga accggatcag cttgccggcg cgcgccttgc tgtcgccgcc cttgaggtgg
     4861 gcccgccact ccttctcgac cgccggccac gggatgccga gcgcttcggc caccgcgcgc
     4921 ttggcgctct tgccatcgcg ctggctgacc agcgcgtcgc ggatcccgcg gtagccgatc
     4981 ttgctctgca tccagccgac cagggtgtac acctcggcgt aggcgagcgc ggcggcctcc
     5041 tggctgggca gcttggccat cgacgggtgc atctcctcga acgtgatcag ccggcccttg
     5101 cgcagcgcgg cggacagcag ggcctgctcg gtggtcgaca gctggagctc ggggcccttg
     5161 cgccaccggg tctgctccag ccgcgccagg ccctcctgca gccacaccgg cacggcgtcg
     5221 tgcgcgaccc gcgacaccac gaggtgggtg tactcgtggg cgagcgtgtc catccacggg
     5281 tagccgaaga tcgtggcgcg cggcgacacg accatgagct tgttgtactt cgacagcgcg
     5341 atcgtgccgg tggtctcgat gtcggtctc
//
LOCUS       DASVOR010000067         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205289,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000067 DASVOR010000000
VERSION     DASVOR010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205289"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF516_01520"
     CDS             complement(<1..272)
                     /locus_tag="VF516_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX8106368.1"
                     /translation="MPRLDYLLATDDALIAQCDVDRYRASGPGGQHRNKTESAVRLRH
                     RLSGVTAIGEDSRSQAENKVHAVRRLRAAIALAVREPVALDGYAPSP"
     gene            complement(308..1246)
                     /locus_tag="VF516_01525"
     CDS             complement(308..1246)
                     /locus_tag="VF516_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106369.1"
                     /translation="MSSLDLALQRALSVRLNLQISLSQLAIVEEWMDIHLDRWLEHLP
                     MPPDMPDGQAVSYLAMLGSDLHRIWWGAWGDPRGFVPKMADYFKLCNIARSDAALLDQ
                     VGEQLEPKLVGSWIGVAGGKVITGWHFWDPHDWSKVETMFGTHEAKYQIKKWVDDHHV
                     ERIERFTQSIGESAFSEVELAVPGATIDDQVNALDAAFAGFTGAPLPPALVTGLRAIT
                     SPQFALAFRIRGGKITRLGALLPGISIDDIQRMCGDARVGVDPRLGKLVGALGEGIAR
                     VEYGRAGDRAGVDVYLEPTDAATRAPEAAAPSAEAN"
     gene            complement(1328..1864)
                     /gene="moaB"
                     /locus_tag="VF516_01530"
     CDS             complement(1328..1864)
                     /gene="moaB"
                     /locus_tag="VF516_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HEX8106370.1"
                     /translation="MEPTFIPVHVAVLTVSDTRTLETDSSGAYIADRLTEVGHRIVAR
                     AIVKDGELVIRAQLAQWIADPGVDVVIATGGTGVTPRDVTPEALAPLVTKPIPGFGEL
                     FRWLSFQEIGTSTIQSRAEAALCGQTYVFLLPGSTGGVRTGIEKILIPQLDHRQKPCN
                     FVMLLPRIKNEPTGLGSR"
     gene            1987..2379
                     /locus_tag="VF516_01535"
     CDS             1987..2379
                     /locus_tag="VF516_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106371.1"
                     /translation="MKEEPMTMKEVSMPAPKIETSSPRQGVLPGVVHLAIDVAEKGQS
                     TAIAVLQDVRVELRGAIEQGIELAEKVSAAGFRLARKAVQRIDEAANETLTGAERLLG
                     GAVKSARETTNAAAQLATTAVAGVAASA"
     gene            2472..2714
                     /locus_tag="VF516_01540"
     CDS             2472..2714
                     /locus_tag="VF516_01540"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX8106372.1"
                     /translation="MKVKASVTLSEDLLRAVDELARSSSRSEVIERALREFFAARARA
                     ERDARDLEIMNAHADAYNVETADLTTYQTWIDDLAE"
     gene            2704..3099
                     /locus_tag="VF516_01545"
     CDS             2704..3099
                     /locus_tag="VF516_01545"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX8106373.1"
                     /translation="MPSKPGRPLPSRAAESAGHPWGAQLRRGELYRVRHPSGDTKRSR
                     VVVVVSRPVHLASRFSSAICAPVYSQRGGLSTEVEVGPAEGLKHPSAILCDVLTSFPR
                     SQLTDYVGTLGPAKLAALRAALRIAVDVE"
     gene            complement(3118..>4213)
                     /locus_tag="VF516_01550"
     CDS             complement(3118..>4213)
                     /locus_tag="VF516_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8106374.1"
                     /translation="WAGSCWYYLRFLSPQRDDVAWDADEERYWMPVDLYVGGNEHAVL
                     HLLYARFWHKVLYDAGLVSTKEPFQRLFHQGMIHKPSFKDARGKYHAAGEVEARGDTA
                     VDPAKANFAEVSAASWVLKGTGDPVETKLDKMSKSRYNVVNPDDMAAEYGADAMRLYE
                     LFMGPLEDGVEWETSGVAGTRRFLDRVWRLLVDAETGALSGKVSEDAPTGNRELERAL
                     HAAIKKVTQSVADLRFNTAIAEMMVFVNEATKAPAVPRDWFEAFVKILSPFAPHLGEE
                     LWQRLGHAGSIAYAPWPAHDEAKLARDTMVIAVQVSGKLRGQIEVAPDAAEADILAAA
                     QADPKVQSFVAGKPVKKAIYVKGRLVNLVV"
BASE COUNT          602 a         1519 c         1453 g          639 t
ORIGIN      
        1 ggcgacggtg cgtagccgtc gagcgcgacc ggctcgcgga ccgccagcgc gatcgccgcg
       61 cgcagccggc gcaccgcatg gaccttgttc tcggcctgcg accggctgtc ctcgccgatc
      121 gcggtgacgc cggacagccg atgccgcagc cggaccgcgc tctcggtctt gttgcgatgc
      181 tggccgcccg ggcccgacgc gcggtagcgg tcgacgtcgc actgcgcgat cagcgcgtcg
      241 tcggtcgcca gcaagtagtc gaggcgcggc atcgagcgtg tcctggctgg cctcgcaacc
      301 gggcccgcta gttggcctcg gccgacggcg ccgccgcctc cggcgcgcgg gtcgccgcgt
      361 cggtcggctc gaggtagacg tcgaccccgg cgcggtcgcc cgcccggcca tactcgacgc
      421 gcgcgatgcc ctcgccgagc gcgccgacca gcttgccgag ccgcggatcg accccgaccc
      481 tggcgtcgcc gcacatccgc tggatgtcgt cgatcgagat cccggggagc agcgcgccga
      541 gccgcgtgat cttgccgccg cggatccgga acgcgagcgc gaactgcggc gaggtgatcg
      601 cgcgcagccc ggtgaccagc gcgggcggca gcggcgcgcc ggtgaagccg gcgaacgcgg
      661 cgtcgagcgc gttgacctgg tcgtcgatcg tcgcgcccgg caccgcgagc tcgacctcgg
      721 agaacgccga ctcgccgatc gactgggtga accgctcgat ccgctcgacg tggtgatcgt
      781 cgacccactt cttgatctgg tacttcgcct cgtgggtgcc gaacatggtc tcgaccttgg
      841 accagtcgtg cggatcccag aagtgccagc cggtgatcac cttgccgccc gccacgccga
      901 tccacgagcc gaccagcttg ggctcgagct gctcgccgac ctgatcgagc agcgcggcgt
      961 cgctccgcgc gatgttgcac agcttgaagt agtccgccat cttgggcacg aagccgcgcg
     1021 gatcgcccca cgcgccccac catatccggt gcagatcgct gccgagcatc gcgaggtagc
     1081 tgaccgcctg cccgtcgggc atgtcgggcg gcatcggcag gtgctcgagc cagcgatcga
     1141 ggtggatgtc catccactcc tcgacgatcg ccagctgcga caggctgatc tgcaggttca
     1201 gccggaccga gagcgcacgc tggagtgcga ggtcgagcga gctcatcgat ccatgtttat
     1261 cacgcggcca gcgccgggcg ccgtcgcgtg ccaccgcgtg ccgccgtgag ccggcgctcc
     1321 cgcgtcgtca ccggctgccg aggccggtcg gctcgttctt gatccgcggg agcagcatca
     1381 cgaagttgca cggcttctgc cggtggtcga gctgcgggat caagatcttc tcgatcccgg
     1441 tgcgcacacc accggtcgat cccggcaaca ggaacacata ggtctggccg cacagcgcgg
     1501 cctccgcgcg gctctggatc gtcgacgtcc cgatctcctg gaacgacagc cagcggaaca
     1561 gctcgccgaa cccggggatc ggcttggtga ccagcggcgc gagcgcctcg ggggtgacgt
     1621 cgcgcggcgt gacgccggtg ccgccggtgg cgatcacgac gtcgacgccg ggatccgcga
     1681 tccactgcgc gagctgcgcg cggatcacca gctcgccgtc cttgacgatc gcgcgcgcga
     1741 cgatgcggtg gcccacctcg gtcagccgat cggcgatgta cgcgcccgag ctgtcggtct
     1801 ccagcgttcg ggtgtccgag accgtgagga ccgcgacgtg gaccggaatg aaggtgggct
     1861 ccatgactgc tagcttagcg ccactccaga agtttcttcc tcaggtaact tcttacgacg
     1921 cgtcgcgtta ggtgatgtca tcgaaccgtc actgcgatgt gacgcaatgc gtcttagatt
     1981 gccggcgtga aggaggaacc tatgaccatg aaggaggtct ccatgcccgc tccgaagatc
     2041 gaaacgtcgt ctcctcgcca gggtgtcctg cccggtgttg tccacctcgc gatcgacgtc
     2101 gccgagaaag gccagtcgac cgcgatcgcc gtgctgcagg acgtgcgcgt cgagctgcgc
     2161 ggcgcgatcg agcaaggcat cgagctggcc gagaaggtct cggccgccgg cttccggctc
     2221 gcccgcaagg ccgtgcagcg catcgacgag gccgcgaacg agacgctgac cggcgcggag
     2281 cgcctgctcg gcggcgcggt caagtcggcg cgcgagacga ccaacgcggc cgcgcagctc
     2341 gcgaccaccg cggtcgccgg cgtcgccgcg tcggcctgag ccgcacccgg gtcaccctgc
     2401 ccgcctccgg gccatccccg ggggatcggg cgggttgaca tccgtcctac cacaggtagg
     2461 atgccggtag gatgaaagtg aaggcgtcgg tcacactgtc ggaagacctg ctccgggcgg
     2521 tcgatgagct cgcccggtcg tcgagccgct ccgaggtgat cgagcgcgcg ttgcgcgagt
     2581 tcttcgccgc ccgggcgcga gccgagcgcg acgcgcgcga tctggagatc atgaacgcgc
     2641 acgccgacgc gtacaacgtc gagaccgcgg atctgacgac ctaccagacc tggatcgacg
     2701 accttgccga gtaagccggg gcgtccgctg ccgtctcgcg cggccgagag cgcagggcac
     2761 ccctggggcg cgcagctccg gcgcggcgag ctgtaccgcg tccggcatcc cagcggcgac
     2821 accaagcgca gccgcgtcgt cgtggtggtc agccgcccgg tgcacctggc atcgcggttc
     2881 tcgtccgcga tctgcgcgcc ggtctactcg cagcgcggcg gcctgtcgac cgaggtcgag
     2941 gtcggccccg ccgaggggct caagcacccg tcggcgatcc tgtgcgatgt gctcaccagc
     3001 ttcccgcgca gccagctcac cgactacgtc ggcacgctcg gccccgccaa gctcgccgcg
     3061 ctgcgcgccg cgctgcggat cgcggtcgac gtggaatgac ggcgcggcgg acgcccgtca
     3121 caccacgagg ttgaccagcc ggcccttgac gtagatcgcc ttcttgaccg gcttgccggc
     3181 gacgaacgac tgcaccttgg ggtcggcctg cgccgcggcc aggatgtcgg cctcggcggc
     3241 gtcgggcgcg acctcgatct ggccgcgcag cttgccgctg acctgcaccg cgatgaccat
     3301 cgtgtcgcgc gcgagcttgg cctcgtcgtg ggccggccac ggcgcgtacg cgatcgaccc
     3361 ggcgtggccg agccgctgcc acagctcctc gccgaggtgc ggcgcgaacg gcgacaggat
     3421 cttgacgaac gcctcgaacc agtcgcgcgg caccgccggc gccttggtcg cctcgttgac
     3481 gaacaccatc atctcggcga tcgcggtgtt gaaccgcagg tcggcgaccg actgggtgac
     3541 cttcttgatc gccgcgtgca gcgcgcgctc gagctcgcgg ttgccggtgg gcgcgtcctc
     3601 gctgaccttg ccggacagcg cgccggtctc ggcgtcgacc aggagccgcc acacccggtc
     3661 gaggaaccgc cgcgtgccgg cgaccccgga cgtctcccac tcgacgccgt cctccagcgg
     3721 gcccatgaac agctcgtaga gccgcatcgc atcggcgccg tactcggcgg ccatgtcatc
     3781 ggggttgacc acgttgtatc ggctcttcga catcttgtcg agcttggtct cgaccgggtc
     3841 gcccgtgccc ttgaggaccc agctcgccgc gctgacctcc gcgaagttgg ccttcgccgg
     3901 atcgaccgcg gtgtcgccgc gggcctcgac ctcgccggcc gcgtggtact tgccccgcgc
     3961 gtccttgaac gacggcttgt ggatcatgcc ctggtggaac aggcgctgga acggctcctt
     4021 ggtcgacacc agcccggcgt cgtacagcac cttgtgccag aaccgcgcgt agagcaggtg
     4081 gagcaccgcg tgctcgttgc cgccgacgta gaggtcgacc ggcatccagt agcgctcctc
     4141 gtcggcgtcc cacgcgacgt cgtcgcgctg cggcgacagg aaccgcaggt agtaccagca
     4201 cgagcccgcc cac
//
LOCUS       DASVOR010000068         3375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_234465,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000068 DASVOR010000000
VERSION     DASVOR010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3375
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234465"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1625
                     /locus_tag="VF516_01555"
     CDS             96..1625
                     /locus_tag="VF516_01555"
                     /inference="COORDINATES: protein motif:HMM:NF005686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8106375.1"
                     /translation="MPRCGCRIGGGDPRRRAASLPHRGTAGTAAAAPDLVRLDARRLS
                     ELIHARRASCVEVMTAYLDQIERLNPQVNAIVSLRPRDALLREAAERDAALERGESAG
                     WLHGVPIAVKDLLPTRGIRTTWGSPLFAEFVPDADAIAVERMRRAGAIVIGKTNVPEF
                     GLGSQTYNDVFGTTRNAYDPARTAGGSSGGAAVALALRMLPIADGSDHAGSLRNPAAF
                     NNVLGFRTTFGCVPSAEGDVFLAALGVIGPMARTATDLALLLSVLAGCDPRAPLSSPG
                     SPARFAAPLDRDLAGTRIGWLGDLGGHLPFEPGVLELARAALPAFEAIGCTVDEVRLD
                     YSMERVWQSWLVLRAYQVGSVLKVHHTDPDKRARMKPEARWEVERGAKLTAYDLADAQ
                     TERTAWYHVVRGLFDRFDYLVLPSAQVFPFDAAVHWPREIAGRAMDTYHRWMEVVVPI
                     TMAGGPALNVPAGFNPGGLPMGIQLVGRPHDELGCLQLAHAYEQATGWVARRPPPLAG
                     V"
     gene            complement(1629..2216)
                     /locus_tag="VF516_01560"
     CDS             complement(1629..2216)
                     /locus_tag="VF516_01560"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8106376.1"
                     /translation="MADPARQPRTFEQLYDEILRLPEGLTGEILEDGVLRVMSRPGKR
                     HRRAALACLDALSGVNANLRGTGWWIEVEAEIRLLQERLAVPDLAGWRVERVPDLPDE
                     NPLTVVPDWCCEILSPRTARDDKRLKLPLYARAGIPWIWQVDPTLQLIEVYQTINGLP
                     ALVTTAQEAERCVLPPFELEISLEQWWLPGPPLTE"
     gene            complement(2349..2837)
                     /locus_tag="VF516_01565"
     CDS             complement(2349..2837)
                     /locus_tag="VF516_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106377.1"
                     /translation="MKGLLLIAAISLGACAHQVAYVPGTRVLYSAPNKSVLDACEEYR
                     LAVERGDADALMLMAHKQYWEDSGTPSGSDDYGIEGLRTVLLTRLQKASDIRYSMRYV
                     AVHQQCTELKPGCRAAVDVLIDASFTIANPLGRPQRPDKRDQNQLVLEWDGHRWLFLS
                     GM"
     gene            complement(2881..>3375)
                     /locus_tag="VF516_01570"
     CDS             complement(2881..>3375)
                     /locus_tag="VF516_01570"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8106378.1"
                     /translation="FSASSDPALARAIAVIGEGLAAAEAATAVRRAASHGIIHGDLFR
                     DNVLWDAGRLTAILDFEQASGGSLAYDLAVCLNDWCWDGGFRRDVAAALLAGYQRVRP
                     LPAADREAFLIEVPAAATRFTLTRITDVYLAKVDNPDKDFRAFLARCDAWRGPALGEL
                     RSLL"
BASE COUNT          474 a         1223 c         1208 g          470 t
ORIGIN      
        1 cgagccgcca gggcaccgcg cggggcgagc gcgaatagcc gcccggcgcc ggcggttgca
       61 agggcatgca tcgccggggc agggaccgca ccgggatgcc gcgttgtggc tgtagaatcg
      121 ggggtggtga cccgcggcgc cgcgcagcat ccctccccca ccggggcacg gccgggaccg
      181 ccgccgctgc tccggacctc gtccggctcg atgcccgccg gctgtccgag ctgatccacg
      241 cccgccgggc gtcgtgcgtc gaggtcatga ccgcctacct cgatcagatc gagcggctga
      301 acccgcaggt caacgcgatc gtctcgctgc ggccgcgcga tgcgctgctc cgcgaggccg
      361 ccgagcgcga tgcggcgctc gagcgcggcg agtcggcggg ctggctgcac ggcgtgccga
      421 tcgccgtcaa ggacctgcta ccgacccgcg gcatccgcac cacgtggggc tcgccgctgt
      481 tcgccgagtt cgtccccgac gccgacgcga tcgcggtcga gcgcatgcgg cgcgccggcg
      541 cgatcgtcat cggcaagacc aacgtccccg agttcggcct cggctcgcag acctacaacg
      601 acgtgttcgg caccacgcgc aacgcctacg acccggcgcg cacggcgggc ggcagcagcg
      661 gcggcgccgc ggtcgccctc gccctgcgca tgctgccgat cgccgacggc agcgatcacg
      721 cgggctcgct gcgcaacccg gccgcgttca acaacgtgct cggcttccgc accacgttcg
      781 gctgcgtgcc gtccgccgag ggcgacgtct tcctcgcggc gctcggcgtg atcggcccga
      841 tggcgcgcac cgccaccgat ctcgcgctct tgctctcggt gctcgccggt tgcgatccgc
      901 gcgcgccgct gtcgagcccg ggcagcccgg cccggttcgc cgcgccgctc gatcgcgacc
      961 tcgccggcac gcggatcggc tggctcggcg atctcggcgg ccacctgccg ttcgagcccg
     1021 gcgtgctgga gctcgcccgc gcggccctgc ccgcgttcga ggcgatcggc tgcacggtcg
     1081 acgaggtccg cctggactac tcgatggagc gcgtgtggca gagctggctg gtgctgcgcg
     1141 cgtaccaggt cggctcggtg ctcaaggtcc accacacaga tcccgacaag cgcgcgcgga
     1201 tgaagcccga ggcgcgctgg gaggtcgagc gcggcgccaa gctgaccgcc tacgacctcg
     1261 ccgacgcgca gaccgagcgc accgcgtggt accacgtcgt gcgcggcctg ttcgatcgct
     1321 tcgactacct ggtgctgccg tcggcgcagg tgttcccgtt cgacgcggcc gtccactggc
     1381 cgcgcgagat cgccggccgc gcgatggaca cctaccaccg ctggatggag gtcgtcgtgc
     1441 cgatcacgat ggcgggcggc cccgcgctca acgtccccgc cggattcaac cccggcggcc
     1501 tgccgatggg catccagctg gtcggccgcc cgcacgacga gctcggctgc ctgcagctcg
     1561 cccacgccta cgagcaggcg accggctggg tcgcccgccg ccccccgccg ctcgccggcg
     1621 tgtagcgcct actcggtcag cggcgggccc ggcagccacc actgctccag cgagatctcg
     1681 agctcgaacg gcggcagcac gcaccgctcc gcttcctgcg ccgtggtcac cagcgccggc
     1741 aggccgttga tggtctgata gacctcgatg agctgcaggg tcggatcgac ctgccagatc
     1801 cagggaatgc ccgcgcgcgc gtacagcggc agcttgagcc gcttgtcatc gcgcgcggtc
     1861 cgaggcgaca ggatctcgca gcaccagtcg gggaccacgg tcagcgggtt ctcgtcgggc
     1921 agatcgggga ctcgctcgac gcgccagccg gcgagatcgg gaacggcgag gcgctcctgg
     1981 agcaggcgga tctcggcttc gacctcgatc caccagccgg tccctcgcaa gttggcgttg
     2041 acgccggaca gcgcatcgag acaggccagt gcggcgcggc ggtggcgctt gccgggccgc
     2101 gacatcaccc gcaggacgcc atcctcgagg atctccccgg tcaggccctc ggggagccgc
     2161 aggatctcgt cgtagagctg ctcgaacgtg cgaggctggc gcgccggatc ggccatgctt
     2221 ccaacgtacc gcagcgatca cgaacctgca ctcttcatcc ccgcagcgcg gcgttttact
     2281 ggagcgtcga agcgctgaca atcaccgacg ctgccgagcc gctcctcgcc ggagcccgat
     2341 gctcgtgcct acatgccgct caggaacagc cagcggtgac cgtcccactc gaggacgagc
     2401 tggttctgat cgcgcttgtc ggggcgctgc gggcggccga gcgggttggc gatcgtgaag
     2461 ctggcgtcga tcaggacgtc gaccgcggcg cggcagccgg gcttgagctc ggtgcactgc
     2521 tggtggacgg cgacgtagcg catcgagtac cggatgtcgc tggccttctg cagccgggtc
     2581 agcagcacgg tgcgcaggcc ctcgatgccg taatcgtcgc tgccggacgg cgtgccgctg
     2641 tcctcccagt actgcttgtg cgccatcagc atcagcgcgt cggcgtcgcc gcgctcgacc
     2701 gccaggcggt actcctcgca ggcgtcgagc acggacttgt taggcgccga gtacaggacc
     2761 cgcgtgccgg gaacgtaagc gacctggtgc gcgcaggcac cgagcgagat cgccgcaatg
     2821 agcaacagcc ccttcatgtc agcgtcgcgt ggttgcagga gatgcgccaa ggcccggacg
     2881 ctacaacagc gacctaagtt cgccaagtgc tgggccccgc cacgcgtcgc agcgcgccag
     2941 aaaggcacgg aagtccttgt cggggttgtc gacctttgcc aaatagacat cggtgatgcg
     3001 cgtgagggtg aaccgggtcg cggcggccgg aacctcgatc aggaacgctt cacggtcggc
     3061 ggcggggagc gggcggacgc gctggtagcc ggccaggagc gccgcggcga catcgcggcg
     3121 gaagccgccg tcccagcacc agtcgttgag gcacacggcg aggtcgtagg cgaggctgcc
     3181 gccggacgcc tgctcgaagt cgaggatcgc ggtgagccgg ccggcgtccc acaggacgtt
     3241 gtcgcggaac aggtcgccgt ggatgatgcc gtggctggcg gcgcggcgca ccgcggtggc
     3301 ggcctcggcg gcggccaggc cctcgccgat cacggcgatc gcgcgggcga gcgccggatc
     3361 ggacgacgcc gagaa
//
LOCUS       DASVOR010000069         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_293004,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000069 DASVOR010000000
VERSION     DASVOR010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293004"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..758)
                     /locus_tag="VF516_01575"
     CDS             complement(129..758)
                     /locus_tag="VF516_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8106379.1"
                     /translation="METVEPRAAPAPDVEPPDTDGVRGEDPDHDIIALIQRGERAAAL
                     TSLMRRHGTAVYRYCREELHDRTLADDVHQQIFIQAHRDLERFAARSTLRTWLFAIAR
                     HRVLDAVKSRRRAEAHIEEDDTADTPDPTPPPGERLDEARLQHALILCLRKLGEHIRG
                     TLVLRYQQGFTFEELAAIFGEKPGTLQARVTRALPVLRACIEARTGGKL"
     gene            complement(904..1998)
                     /gene="serC"
                     /locus_tag="VF516_01580"
     CDS             complement(904..1998)
                     /gene="serC"
                     /locus_tag="VF516_01580"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF003764.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HEX8106380.1"
                     /translation="MSGRAFNFASGPATLPEPVLRRAQAALWDLDGTGIGILEHSHRG
                     KAFRAVLERTTAVVREVGGIGDEFAVLFMTAGATHHFTLVPQNFLDQSPGGGPEDGAT
                     VDICHTGVWTAKVMEETRRFGKLHLACDAGPTFDATPDAVRYSASPRYVYYVSNETVY
                     GTQWQAPPARPPAPLVCDASSDIFSRPLPLAGHGLIFAGAQKNLGPSGISLVIVRRDF
                     LATRRQGLPPLSDYQTFDREGSMYNTPNTFGIYMIGEVCTWIRDQGGLVAMAERNARK
                     ARLLYDFLDNSRRWRAHARPGSRSTMNVTFRGASAELEAELLARAEERGLSGLAGHRS
                     IGGLRASIYNAFPEEGVRRLVDLLGELDRP"
     gene            complement(2082..2333)
                     /locus_tag="VF516_01585"
     CDS             complement(2082..2333)
                     /locus_tag="VF516_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106381.1"
                     /translation="MSANQANTSGQVTLRRDVKFNGVKVFSATMVADRDQLGEKVTNW
                     MANNPQLKVTDIIITQSSDEAFHCIAITVFFWEDTSPRR"
     gene            complement(2362..3612)
                     /locus_tag="VF516_01590"
     CDS             complement(2362..3612)
                     /locus_tag="VF516_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106382.1"
                     /translation="MKVCPRCFTRFPDGERFCLHDSAILVEEDDIARLGTSIGNYRLD
                     KILGRGGMGTVYSGEHIYIKKPVAVKVLHAQFARYQEAIHRFLREARAATSINHPNIV
                     DVTDFGILADGPVYFVMEYLDGKSLEDLIEKEGAVELHRALNIANQIALALEAAHAVG
                     VIHRDLKPDNIMLLKRPGRRDLLRMAPDQSWITEREESYDFVKVLDFGIAKVLVQDEL
                     LAETVQGAVFGTPEYMSPEAARGEDVDHRADVYSLGVILFDMLCGRPPFEAPQSNEVL
                     AMHIHNNPPSPREFAPHREITEMAEAVILRAMQKDPARRYQNMAEFRRDLANAYGSIS
                     YRRHGAPAPGLTPRGEEARTKKRLTEELDEWITGDASRLSLEQARMLSLVETAEKAFT
                     PGAMSPQEAERLANALDRALEDDE"
     gene            complement(3614..4681)
                     /locus_tag="VF516_01595"
     CDS             complement(3614..4681)
                     /locus_tag="VF516_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106383.1"
                     /translation="MVMLPSLSRGLRLALVLALSVGYVTWHGHLRPAAAAPKGGGDDD
                     DSGDDDDSGAKDEGDDGAEDDKDQPPVTAGGLFTMKTYPVSELLRPLTITQNITQARL
                     ALGTDLSSLGAFSSLGASLEGEYGVSDNFMVIGGFTSAYNFAQFGVYGGFEGALLYDL
                     VDIRLAANVHRFAIPHFCGDTRDGTPADACKDPMDTTKFLSLPNGKYDAGGTQFSLDL
                     GFPFRYDVKPEIAIIALQTLMSIDFNAAKSNVFNPATGMTTSTVTNKATPDLLPSVGI
                     ATNPIPQLSLVVFAQLRVVDFNTDAGNFQVPVTGRVEFSPNQQLDIGLEFTLLNIKPP
                     DPQKPFDNRFLSLFVQSRFGK"
     gene            complement(5010..5375)
                     /locus_tag="VF516_01600"
     CDS             complement(5010..5375)
                     /locus_tag="VF516_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106384.1"
                     /translation="MGEKIFKTGIKRDNDLMYYIKGGDVWATPRKKPGQPKGKAQKVA
                     AAGIEMDYSKYLYYLDGDGDVARKERQVGGSKRKSKKAAPAKKAPARKAPAKKAPAKK
                     APAKKAPAKKMGAKKGKKK"
BASE COUNT          863 a         1940 c         1964 g          961 t
ORIGIN      
        1 gccccgggcc caccaggacc ggcgccgcgg ctcgaccgcc gccagcacgc gggcctccca
       61 gcccggcggc ggctcgaggt cggcgcccag ctgcgcgatc gcacgctcca gtctcttgtt
      121 cgaagccgtc atagcttccc tccggtccgg gcctcgatgc aggcacggag caccggcagt
      181 gcgcgcgtca cccgggcctg cagcgtgccc ggtttctcac caaagatcgc tgccagctcc
      241 tcgaaggtga agccctgctg gtagcgcagc accagcgtgc cacggatatg ctcgccgagc
      301 ttgcggaggc acaggatcag cgcgtgttgc aggcgcgcct cgtcgagccg ctcgcccggc
      361 ggcggggtcg gatcgggcgt gtcggcggtg tcgtcctcct cgatgtgcgc ctccgcgcgg
      421 cgccgcgact tgacggcgtc gagcacgcgg tggcgcgcga tcgcgaacag ccaggtgcgc
      481 agcgtcgagc gcgccgcgaa tcgctccagg tcgcggtgag cctggatgaa gatctgctga
      541 tgcacgtcgt cggccagcgt gcggtcgtgc agctcctcgc gacagtaccg gtacaccgcg
      601 gtaccgtgcc ggcgcatcag gctggtgagc gcagccgcgc gctcgccgcg ctggatcagc
      661 gcaatgatgt cgtgatcggg gtcctcgccg cgcactccgt cggtgtccgg cggctcgacg
      721 tcgggcgcag gcgccgctct cggttccaca gtttccatga ccgtcgctga taggcgacgg
      781 cggacaccgg accacgacac acccgaatcc ggttcggcgc attgcggcaa aggcgcgaac
      841 ggccagggcc gatcgtggcg ggccggccgg ccgggatcgg atctatgacc gacccacctg
      901 gctctaaggt cggtccagct cgccgaggag gtcgaccagc cggcgcacac cctcctcggg
      961 gaacgcgttg taaatactcg cgcgcagacc gccgatcgag cggtgcccgg cgagcccgct
     1021 caggccgcgc tcctcggcgc gggcgagcag ctccgcctcg agctccgccg acgcgccgcg
     1081 gaacgtcacg ttcatggtcg accggctgcc ggggcgggca tgcgcgcgcc accgccggct
     1141 gttgtcgagg aagtcataga gcagcctggc cttgcgcgcg ttgcgctcgg ccatcgctac
     1201 gagcccgccc tgatcgcgga tccacgtaca gacctcgccg atcatgtaga tgccgaacgt
     1261 gttcggcgtg ttgtacatcg agccttcgcg gtcgaaggtc tgatagtccg acaggggcgg
     1321 caacccttgc cggcgggtcg cgaggaagtc ccggcggacg atcaccaggc tgatccccga
     1381 gggcccgagg ttcttctggg cgccggcgaa gatcagcccg tggccggcga gcggcagcgg
     1441 ccggctgaag atgtcgctcg aggcatcgca caccagcggc gcgggcgggc gagccggcgg
     1501 cgcctgccat tgcgtgccgt acaccgtctc gttgctgacg tagtacacgt agcgcgggga
     1561 tgccgagtag cggaccgcgt cgggggtggc atcgaacgtc gggccggcgt cgcacgccag
     1621 gtggagcttg ccgaaccgcc gggtctcctc catgaccttg gcggtccaca ccccggtgtg
     1681 acagatgtcg acggtcgccc catcctccgg gccgcccccc gggctctgat cgaggaagtt
     1741 ctggggcacc agcgtgaagt ggtgggtcgc gcccgcggtc atgaacagga ccgcgaactc
     1801 gtcgccgatc ccgccgacct cgcggaccac cgcggtggtc cgttccagca ccgcgcggaa
     1861 cgccttgccg cggtggctgt gctccaggat gccgatcccg gtaccgtcga ggtcccacag
     1921 ggccgcctgg gcgcgccgca gcaccggctc gggcagcgtc gccggtcccg acgcgaagtt
     1981 gaacgctcta ccactcacgg gtcacggcgg gtcaggctcg tcggcgggcg gcgcatcttt
     2041 cgaacctagg tcatcacgga cggggcatca ccggcgcggc atcaccggcg gggcgacgtg
     2101 tcttcccaga agaacaccgt gatcgcgatg cagtggaacg cctcgtccga cgattgcgtg
     2161 atgatgatat cggtgacctt gagctgcgga ttgttggcca tccagttcgt gaccttctcc
     2221 ccgagctgat cgcgatccgc gaccatcgtc gcagagaaca ccttcacccc gttgaacttc
     2281 acatcccgcc gcagcgttac ctgcccggat gtattcgctt ggttcgcgga catgcggggt
     2341 ggacgatatc atggatgtgg tttactcgtc gtcctcgagc gcgcgatcca gcgcgttggc
     2401 gagccgctcg gcttcctgcg gcgacatcgc accgggggtg aacgccttct cggcggtctc
     2461 gaccagcgac agcatgcgcg cctgctccag cgacagccgg gacgcgtcgc cggtgatcca
     2521 ctcgtcgagc tcctccgtga ggcgcttctt ggtccgcgcc tcctcgccac gcggggtcag
     2581 accgggcgcc ggcgcaccgt gccggcgata gctgatcgag ccgtacgcgt tggcgaggtc
     2641 gcgccggaac tcggccatgt tctggtaccg ccgggcgggg tccttctgca tcgcccgcag
     2701 gatcaccgcc tcggccatct ccgtgatctc gcggtgcggc gcgaactcgc ggggcgacgg
     2761 cgggttgttg tggatgtgca tcgccagaac ctcgttcgac tggggggcct cgaacggcgg
     2821 tcggccgcac agcatgtcga acaggatcac gccgagcgag tacacgtcgg cgcggtggtc
     2881 gacgtcctcg ccgcgcgcgg cctccgggga catgtactcg ggggtgccga acaccgcgcc
     2941 ctgcacggtc tcggcgagca gctcgtcctg gaccagcacc ttggcgatcc cgaaatcgag
     3001 caccttcacg aagtcgtaag actcctcgcg ctcggtgatc cacgactggt cgggggccat
     3061 gcggagcagg tcgcgccggc ccggccgctt gagcagcatg atgttgtcgg gcttgaggtc
     3121 gcggtggatc acgccgaccg cgtgcgcggc ctccagcgcg agcgcgatct ggttggcgat
     3181 gttgagcgcg cggtggagct cgaccgcgcc ctccttctcg atcaggtcct cgaggctctt
     3241 gccgtcgagg tactccatca cgaagtacac cgggccatcg gcgaggatgc cgaagtcggt
     3301 gacgtcgacg atgttcgggt ggttgatcga ggtcgccgcc cgggcctcgc gcaggaagcg
     3361 gtggatcgcc tcctggtagc gggcgaactg ggcgtgcagc accttcaccg cgaccggctt
     3421 cttgatgtag atgtgctcgc cgctgtacac ggtgcccatc ccgccgcggc cgaggatctt
     3481 gtcgagccgg tagttaccga tcgaggtgcc gagccgcgcg atgtcgtctt cctcgacgag
     3541 gatcgccgag tcgtggagac agaatcgctc tccatccgga aaccgcgtga agcagcgggg
     3601 gcagaccttc aagctatttt ccgaagcgcg attgaacgaa cagcgacagg aagcggttgt
     3661 cgaacggctt ctgcgggtcg ggcggcttga tgttgagcag cgtgaactcc aggccgatgt
     3721 cgagctgctg gttcggcgag aactcgaccc ggccggtgac cgggacctgg aagttgcccg
     3781 cgtcggtgtt gaagtcgacg acgcggagct gggcgaacac gaccagcgac agctgcggga
     3841 tcgggttggt cgcgatgccg accgacggca acaggtccgg ggtggccttg ttggtgaccg
     3901 tgctggtcgt catgccggtg gcagggttga aaacgttgga cttcgccgcg ttgaagtcga
     3961 tgctcatcag ggtctgcagc gcgatgatcg cgatctcggg cttgacgtcg tagcggaacg
     4021 gaaaccccag atcgagcgag aactgggtgc cacccgcgtc gtacttcccg ttggggagtg
     4081 acaagaactt cgtcgtgtcc atcgggtcct tgcaggcgtc cgccggtgtt ccgtcgcggg
     4141 tatcaccgca aaagtgcggg atcgcgaagc ggtggacgtt ggcggcgagc cggatgtcga
     4201 cgagatcgta gagcagcgcg ccctcgaagc cgccgtagac gccgaactgc gcgaagttgt
     4261 acgcgctggt gaagccgccg atcaccatga agttgtcgga caccccgtac tcgccctcca
     4321 gcgacgcgcc gagcgagctg aacgcgccga gcgagctgag gtcggtcccc agggcgagcc
     4381 gcgcctgtgt gatgttctgc gtgatcgtca gcggccgtag cagctcgctc accggatacg
     4441 tcttcatggt gaacagcccg cccgcggtga ccggaggctg gtccttgtcg tcttcggcgc
     4501 cgtcgtcgcc ctcgtccttg gcgccgctgt cgtcgtcgtc gccgctgtcg tcgtcgtcgc
     4561 cgccgccctt gggggcggcc gccgccggac gaaggtgccc atgccacgtg acgtagccga
     4621 cgctgagcgc cagcaccagc gcgagccgca acccgcggga aagcgaagga agcatcacca
     4681 cctcggccgg aaatgctacc gcaaatccac cggccaggtg cgcgcgacac cgccgaaacg
     4741 aagcgtatgc cgcgccgcgc cctcgggagc aggcgatagc gctccgcgat ggcaatcgtg
     4801 accgagcgcg ccccgcccga caagcggccg ggatgcgacg agaagacgcg cctcggggcg
     4861 caggcccctg gacggagccc gagaccctgc agcacggtga tgacgagggc ccgccacccg
     4921 gtccccggag tctgaccgcc acccgggttc cccggagtct aaccggaggt cgcgggtcga
     4981 cgggcctccg ccaccacccg ctgggccgac tacttcttct tgcccttctt ggcgcccatc
     5041 ttcttggcgg gggccttctt ggccggcgcc ttcttggcgg gagccttctt ggccggcgcc
     5101 ttcctggcgg gagccttctt ggccggcgca gccttcttgc tcttgcgctt gctgccaccg
     5161 acctggcgct ccttgcgcgc cacgtcgccg tcgccgtcga ggtaatagag gtacttgctg
     5221 taatccatct cgatgccggc ggcggcgacc ttctgcgcct tgcccttggg ctggcccggc
     5281 ttcttccggg gcgtcgccca gacgtcgccg cctttgatgt agtacatcag gtcgttgtcg
     5341 cgcttgattc cagtcttgaa gatcttctca cccataaatt cagaccctcc ctaggggtag
     5401 tggggttgaa gcacggtatc cgaagggtct gacaatctcc gcaaaaaata ttcgaaatcg
     5461 tcgtcggaga ccaggagatc tccgtcgagg aacgcccggg ccaccccggc cggggtgggt
     5521 cccgacgagg atcagcctgt ggataaccgg gttctcgacg acgatctgaa atgatcgtaa
     5581 atccccgacg agaaacggga attcctcgtc gagccacccc ccatttcctc gcttcgctcg
     5641 tcgatctcac ggggggaggt gcccccgccc cgccggccag gcggctcgtc gagcccacgg
     5701 gggctttgcc cccgccccgc cggcaaag
//
LOCUS       DASVOR010000070         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_117691,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000070 DASVOR010000000
VERSION     DASVOR010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117691"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF516_01605"
     CDS             <1..291
                     /locus_tag="VF516_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106385.1"
                     /translation="GSLATSVARRSVERVLPGLRACYAAAARASRTAPAIELRLSFEI
                     DENSLATHVATGDVRFGAFARCVAGVAGQIHSTQAPDVGTARVVVVIQFHPS"
     gene            complement(321..863)
                     /locus_tag="VF516_01610"
     CDS             complement(321..863)
                     /locus_tag="VF516_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106386.1"
                     /translation="MRFALLLVVVALGGTAHAEGERALSVGLGWATFSALGKPMGRQA
                     PPTLSPDIGGALVASYEYSISSDIALRGELAGGVFHGGAQAKQSQTSFASLGDAGAVF
                     RFDVLQVVPYLFAGLGGVVSGGGPLDRGADFVLVVGGGVDWLMSRERSLGLEARLASF
                     AGDVTVATFGVRGTLRWGEL"
     gene            1234..>1575
                     /locus_tag="VF516_01615"
     CDS             1234..>1575
                     /locus_tag="VF516_01615"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106387.1"
                     /translation="MLGKDYRVLQVIGTGGMAVVYLVEHQTLLKRFAAKVLSSELASS
                     AEARARFTQEAHAASQLDHENIVTISDFGVTADQRPFFVMELLRGQTLDQRLGDGPMS
                     IEEVVAVAVPVA"
BASE COUNT          239 a          563 c          543 g          230 t
ORIGIN      
        1 ggctcgctgg cgacctcggt cgcgcggcgc agcgtcgagc gcgtgctgcc cgggctgcgg
       61 gcatgctacg cggcggcggc ccgcgccagc cgcacggcgc ccgcgatcga gctccggctg
      121 agcttcgaga tcgacgagaa tagcctcgcg acccacgtgg cgaccggcga cgtccggttc
      181 ggcgcgttcg cgcgctgcgt cgcgggcgtc gccggccaga tccacagcac gcaggcgccc
      241 gacgtcggca ccgcgcgcgt cgtggtcgtg atccagttcc atccgtcgtg accgggcggc
      301 cgcgcggcga gctcgggtga ctagagctcg ccccagcgca gcgtgccgcg caccccgaac
      361 gtcgcgaccg tgacgtcgcc ggcgaagctc gcgagtcgcg cctcgagccc cagcgagcgc
      421 tcgcggctca tcagccagtc gacaccgccg ccgaccacca gcacgaagtc ggcgccgcgg
      481 tcgagcggcc cgccgccgga caccacgccg ccgaggccgg cgaacaggta gggcacgacc
      541 tggagcacgt cgaaccggaa caccgcgccg gcgtccccga gcgaggcgaa cgaggtctgg
      601 ctctgcttgg cctgcgcgcc gccgtggaac acgccgccgg cgagctcgcc gcgcagcgcg
      661 atgtcgctgc tgatcgagta ctcgtagctg gcgaccagcg ccccgccgat gtcggggctc
      721 agcgtcggcg gcgcctgccg gcccatcggc ttgccgagcg cgctgaacgt cgcccagccc
      781 aggccgaccg acagcgcgcg ctcaccctcg gcgtgggcgg tcccaccgag cgcgaccacg
      841 accagtagca gggcgaacct caccgtacgg ttgtatcacc gagcacgcac ggacgctcac
      901 atcggctggc cagatgcgag gtctgcggtg cgcgccgatg ggggatgtcg accatgcgat
      961 ttgtgacgga ccagccgtcg aatgccagtc atttctccgt gcgccgccgc cacgccgccc
     1021 ggcaattcag cagttacgca aagaatgcac cctcgcggtg tatcactcgc tctcacgcca
     1081 ccgtcggact catcacttcc cgacggagcc cggacagcgt gttgtcgatg tcacgttagg
     1141 atcggttgtt cgagggcgat attgcgtgca gaatcgcggt gatgaggtcc gttcgtcgtg
     1201 ctgcctgagc gagttgatgc actcgtcggg tcgatgctcg gcaaggacta tcgcgtgctt
     1261 caggtgatcg gcaccggcgg catggccgtc gtgtatctgg tcgaacatca gacgctgctc
     1321 aagcggttcg ccgccaaggt gctgtcgagc gagctcgcct ccagcgccga ggcgcgggcg
     1381 aggttcacac aagaagcgca cgccgcttcg caactcgatc acgagaacat cgtcacgatc
     1441 agcgacttcg gcgtcaccgc cgatcagcgg ccgttcttcg tcatggagct gctgcgcggc
     1501 cagaccctcg atcagcggct cggcgacggc ccgatgtcga tcgaggaggt cgtcgcggtc
     1561 gcggtgccgg tggcg
//
LOCUS       DASVOR010000071         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439081,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000071 DASVOR010000000
VERSION     DASVOR010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439081"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..547)
                     /locus_tag="VF516_01620"
     CDS             complement(<1..547)
                     /locus_tag="VF516_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106388.1"
                     /translation="MQSLRLVDVHRGADQIVASCALDDLRFHVTVWYEDVDLAALARR
                     FGDEAIERIAVHIALFQLNAACSLRPEVIELGRYARYLTEDLVRLWRTVFHHVWAQWR
                     YEHDLPHYDGPRFVDPVAPPPAPLHAPDGPVELLAFCGGGKDSLVALKLLERAGLPFA
                     TLGYAHSIYGAAEPQHALLDRV"
     gene            775..>1578
                     /locus_tag="VF516_01625"
     CDS             775..>1578
                     /locus_tag="VF516_01625"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX8106389.1"
                     /translation="MSAVVATGCVVQPIPGPRYQQPPPQYAQQDPQGPPPSEPPPEQP
                     YQPPPPQQPAELPPASYSDPVYTDVNVEVAGNDVPSVDVFYDQLSPYGTWYDDPSYGW
                     VFTPSDASYQPYTNGHWTDTDYGLTWVSNDPYGWATDHYGRWVWANRWVWRPDTTWGP
                     AWVQWRQGDGYVGWAPAGYTDDAYFPEEHWRIVPAAYLFTPEPRRYVIRDVHPYLTTT
                     FVVRRYGHDRRNHTWVLGPDREWLQRNRVEVRRERVDPVVLGRFDEQRRR"
BASE COUNT          243 a          564 c          564 g          207 t
ORIGIN      
        1 cgacccggtc gagcagcgcg tgctgcggct cggcggcgcc gtagatcgag tgcgcatagc
       61 cgagcgtggc gaacggcaga ccggcgcgct cgagcagctt gagcgcgacc aggctgtcct
      121 tgccgccgcc gcagaacgcg agcagctcga ccggaccgtc gggggcgtgg agcggcgccg
      181 gcggcggcgc gaccgggtcg acgaaccgcg ggccgtcgta gtgcggcaga tcgtgctcgt
      241 atcgccactg cgcccacacg tggtggaaca cggtgcgcca cagccggacc aggtcctcgg
      301 tcaggtacct cgcgtagcgc ccgagctcga tgacctcggg ccgcaggctg cacgcggcgt
      361 tgagctggaa cagcgcgatg tggaccgcga tccgctcgat cgcctcgtcg ccgaaccgcc
      421 gcgccagggc ggcgagatcg acgtcctcgt accacaccgt cacatgaaac cgcagatcgt
      481 cgagcgcgca gctggcgacg atctggtcgg cgccgcggtg gacgtcgacg agacgcaaac
      541 tttgcatcgc cggagcctag cgcctccgcc gggtgcgacc gagccgcgcg tgcggctccg
      601 gccgggattg tgcgcgagcg caaggtcgtg ccgtcgaggc acgcggggca atgtgcccca
      661 tacccatggt ggggcgaccg gccgcgcctt atcgtggtga atgcgggcgg gggctcgccg
      721 tccacctccc tggcaccgtc gaaaggctcc atgtcgatgc gaatgacaac gtggttgagt
      781 gcagtcgtgg ccaccggctg cgtcgtgcaa ccgattcccg ggccgcggta tcagcaaccg
      841 ccgccgcagt acgcacagca ggacccgcag gggccaccgc cgagcgagcc gccgccggag
      901 cagccctacc agccgccgcc cccgcagcag ccggccgagc tgccgccggc gagctacagc
      961 gacccggtct acaccgacgt caacgtcgag gtcgccggca acgacgtgcc gtcggtcgac
     1021 gtgttctatg accagctgtc gccgtacggc acctggtacg acgatccgag ctacggctgg
     1081 gtgttcacgc ccagcgacgc gtcgtaccag ccgtacacca acggccactg gacagacacc
     1141 gactacggcc tgacctgggt gtcgaacgat ccgtacggct gggccaccga tcactacggc
     1201 cgctgggtgt gggccaaccg ctgggtgtgg cggccggaca ccacgtgggg cccggcgtgg
     1261 gtccagtggc ggcagggcga cggatacgtc ggctgggcgc ccgcgggcta caccgacgac
     1321 gcgtatttcc ccgaggagca ctggcggatc gtgccggcgg cctacctgtt cacgcccgag
     1381 ccgcggcgct acgtgatccg cgacgtccac ccgtacctga cgacgacgtt cgtcgtgcgg
     1441 cgctacggcc acgaccgccg caaccacacc tgggtgctcg ggcccgaccg cgagtggctg
     1501 cagcgcaacc gggtcgaggt ccgccgcgag cgggtcgatc cggtcgtgct cggccggttc
     1561 gacgagcagc gccgccgc
//
LOCUS       DASVOR010000072         1709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_234484,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000072 DASVOR010000000
VERSION     DASVOR010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1709
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234484"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF516_01630"
     CDS             complement(<1..306)
                     /locus_tag="VF516_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106390.1"
                     /translation="MSVLDTGISAAVRDTLHRMLELIEQFDGSAGARQRLIDGVERRV
                     AEVIASRQRGDLSLVARAEAEVRADPGGRIRLDGSGEATVHAVRRVFRGGRFEVPPLG
                     "
     gene            431..556
                     /locus_tag="VF516_01635"
     CDS             431..556
                     /locus_tag="VF516_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106391.1"
                     /translation="MSSPSMVHEGVIALVRDNPAFAASLLRDLLHVEVPHFRGRG"
     gene            840..1130
                     /locus_tag="VF516_01640"
     CDS             840..1130
                     /locus_tag="VF516_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106392.1"
                     /translation="MALIENAWREIVRKALDMDPMIKKFFTDANRQLFAEGRAEGEAE
                     GKGKALMMILGHRGLATPIELSLFLFRRIDPVTLDRWLARALSVASVDEVFM"
     gene            1152..1658
                     /locus_tag="VF516_01645"
     CDS             1152..1658
                     /locus_tag="VF516_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106393.1"
                     /translation="MASQNDARRIAMALPGVTASTERFAFSVENKGKAKGFIWAWNER
                     IDPRKPRVPNPQVLAARVANETEKAALLGADPDKFFTEPHYHGFPAVLVRLPAVSIGE
                     LRQVITEAWRCQAPKDLRAAYDAGDAAPRPKPAAVRPRAASNSRPSAPKRKPARKVAA
                     RSRAHRAR"
BASE COUNT          288 a          599 c          571 g          251 t
ORIGIN      
        1 acccagcggc ggcacctcga accggccgcc gcgaaacacg cggcgcaccg cgtgcaccgt
       61 cgcctcgccg ctaccgtcga gccggatccg gccgcccgga tccgcacgga cctcggcctc
      121 ggcgcgcgcc accagcgaca ggtcgccgcg ctggcgcgac gcgatcacct ccgcgacccg
      181 gcgctcgacg ccgtcgatca ggcgctgccg cgcacccgcg ctgccatcga actgctcgat
      241 cagctccagc atgcggtgca gcgtatcgcg aaccgcggcc gagatcccgg tgtcgaggac
      301 cgacaccgag gcgttgatcg aagcctccgc cacagcgcac gaatcgatgc ttcggcaaga
      361 cgacactcga tcacaatccc caggtggttt gtttcgatcg tcagagctcc tcgctagagt
      421 ggctcccgcc atgtcatctc cgtcgatggt gcacgagggc gtgattgcac tggtccgcga
      481 caacccggca ttcgcggcca gcttgcttcg cgacctgtta catgtcgagg tcccgcactt
      541 ccgcgggcgc ggctgagcga agcggcgctc acgcagctcg tgccggtcga gtaccatgcc
      601 gacgctgtcg tcctgttcga cggcgtcgtc gacgacgaga cgaagccggt gctggggacc
      661 atcttcgagg tccagctcga gcgcaaggat cctctcatgt accgatgtgg ccaccctcga
      721 tcggtggctg gctcgcgcgc tgtccgtggc ctcggtagac gagctcctcg cgtagcccga
      781 cccagacgag accgccgtgg ccaccgagga cgacgcggct tggcttcgag cagaacagag
      841 tggcgctgat cgagaatgcc tggagagaga tcgtacgaaa ggccctggac atggacccca
      901 tgatcaagaa gttcttcacc gacgcgaatc gccagttgtt tgcagagggc agggccgagg
      961 gggaagccga gggcaagggc aaggcactga tgatgatctt ggggcaccgc ggcctcgcca
     1021 cgcccatcga gctttcgctg ttcctcttca ggcgcataga tccggtcacc ctcgatcgat
     1081 ggctggctcg ggcactgtcg gtcgcctcgg tggacgaggt tttcatgtag cgccggggca
     1141 ggcagatcgc catggccagc cagaacgatg cacggcggat cgcgatggcg ctacccggcg
     1201 tcaccgcatc gaccgagcga ttcgcgtttt ccgtcgagaa caagggcaag gccaagggct
     1261 tcatctgggc ctggaacgag cgcatcgatc ccaggaagcc gcgcgtcccg aacccgcagg
     1321 tgctcgcggc gcgggtggcc aacgagaccg agaaggcggc gctgctcggc gccgaccccg
     1381 acaagttctt caccgagccg cactatcacg gctttccggc ggtgctggtc cggctgccgg
     1441 cggtgtcgat cggcgagctg cgccaggtga tcacggaggc gtggcgctgc caggcgccca
     1501 aggatctgcg ggcggcctac gacgcgggcg acgctgcgcc gcggccgaaa cccgccgccg
     1561 tacgcccgcg cgcagcgtcg aacagccgcc cgtccgcgcc gaagcgcaag ccggcacgca
     1621 aggtcgctgc acggagccga gcccaccgcg ctcgatgacg gccgatgccg gcgcgtcgag
     1681 ctcgatccgg cgacgccgtg cgaccaccg
//
LOCUS       DASVOR010000073         2324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_380615,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000073 DASVOR010000000
VERSION     DASVOR010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380615"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VF516_01650"
     CDS             complement(<1..838)
                     /locus_tag="VF516_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106394.1"
                     /translation="MILAEIQYPHSRGAAGLGQGVQQRACAVVADLVVGQAEVGELGE
                     GSERACTVVTDLVAVQIEEGELGKDSEPACAVVVDPVALQIEEGELGESSERACAVSA
                     DRVGGQVEGGELGEGSERACTVVADRVGSEDEGGELGEGSECACAVVANRVVGQVERG
                     ELGEGSECACAIGANLIGREVEVGELGEGSERACAVVADRVAAEAERGEPGEGSECGC
                     AVVADLIVLQIEVGELGESRERVCAVGADGIAAEVERGEPGEGSERTCAVGADLVEPQ
                     VEV"
     gene            818..>2324
                     /locus_tag="VF516_01655"
     CDS             818..>2324
                     /locus_tag="VF516_01655"
                     /inference="COORDINATES: protein motif:HMM:NF027421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COR domain-containing protein"
                     /protein_id="HEX8106395.1"
                     /translation="MYLRENQIAAPLLTTEALVSGDAQAILAAYRRYRDSEREATLRP
                     LNEAKLIVLGSEAVGKTSLIRYLVHDTPRNSDEAKTPGAKIHEQIQVQTWSAHHSPVR
                     LNVWDFGGQEIMRGTHRFFLTARSLYLLVIEDRREDDRSIYDWLETIAQCGGESPVIV
                     VINKTDREVPQRLIDEDALQRSHPAIVSFVRTSCNADPRSAASIVRLRELIATTLALS
                     PRLTHVRDPIPQSWLRVKNAISTEARRRFVLPVRDFEQLCTGSMDVPQAERIADANEQ
                     RAALLLLHDLGVVIAHGARDHATAAWREVSILDPNWLTQAIYSLLTSHVVRDQQGELR
                     RDQLGALLDPERYPARWHELILSMMQDPALGLCLPITRGDAERYLLPEALPPSEPDYG
                     IWPDDSLRFRFQYQRLPAGLLPRLIVEAHRSLTDRPTYWRTGVVLASDGCRILVRGEV
                     GKARVEIHVAGPTHRRSALSVVRGYFDAVHRHYPRLTVNARVPLPEQPEVDV"
BASE COUNT          386 a          924 c          655 g          359 t
ORIGIN      
        1 ccacctcgac ctgaggttca accagatcgg cgccgacggc gcatgtgcgc tcgctgccct
       61 ctcccggctc acctctctcg acctccgccg caattccatc ggcgccgacg gcgcacacgc
      121 gctcgcggct ctcacccagc tcacccacct cgatctggag gacaatgaga tcggcgacga
      181 cggcgcaccc gcactcgctg ccctcccccg gctcacctct ctcagcctcc gcagcaactc
      241 gatcggcgac gacggcgcac gcgcgctcgc tgccctctcc cagctcaccc acctcgacct
      301 cgcggccaat gagattggcg ccgatggcgc acgcgcactc gctgccctca cccagctcac
      361 ctctctcgac ctgtcctaca actcgattgg cgacgacggc gcatgcgcac tcgctgccct
      421 cgcccagctc accaccctcg tcctcactgc caactctatc ggcgacgacg gtgcacgcgc
      481 gctcgctgcc ctctcccagc tcaccgccct ctacctgtcc tccaactcga tcggcgctga
      541 cggcgcacgc gcgctcgctg ctctctccca gctcaccttc ctcaatctgg agggcaaccg
      601 gatcgacgac gacggcgcac gcgggctcgc tgtccttacc cagctcacct tcctcgatct
      661 ggacggcaac gagatcggtg acgacggtgc acgcgcgctc gctgccctct cccagctcac
      721 cgacctctgc ctgtcctaca accagatcgg cgacgacggc gcacgcgcgc tgctggactc
      781 cctgtccgag cccagccgct cctcgcgaat gcggatattg tatctccgcg agaatcaaat
      841 cgcagcccct ctcctgacca ccgaggcact ggtatccggg gacgcacagg ccatcctcgc
      901 ggcgtaccgc cggtatcgcg acagcgagcg ggaggccacg ctgcgcccgc tcaacgaggc
      961 caagctcatc gtcctcggca gcgaagcggt cggcaagacc tcactgatcc gctacctcgt
     1021 ccacgacacg ccgcgaaatt ccgacgaggc gaagacgccc ggggcgaaga ttcacgagca
     1081 gatccaggtc cagacctggt cagcacatca ctcgcccgtg cgcctgaacg tgtgggactt
     1141 cggcggccag gagatcatgc gcgggaccca ccggttcttc ctcaccgcgc gcagcctcta
     1201 cctcctcgtg atcgaggacc ggcgggaaga tgaccgctcg atctacgact ggctcgagac
     1261 catcgcgcag tgcggcggcg agtcaccggt catcgtcgtg atcaacaaga ccgatcgcga
     1321 ggtcccgcag cgcctgatcg acgaggacgc gctgcagcga agccaccccg cgatcgtcag
     1381 tttcgtgcga acgagctgca atgccgaccc gcgctccgcc gcgtcgatcg tccgcctgcg
     1441 cgagctgatc gcgaccacgc tcgccctgtc accacgtctc acccacgtcc gcgacccgat
     1501 cccacagtcg tggctgcgcg tgaagaacgc gatcagcacc gaagcaagac gacggttcgt
     1561 gctgcccgtt cgcgacttcg agcagctttg caccgggagc atggatgtcc cacaggccga
     1621 gcgcatcgcc gacgcgaacg aacaacgcgc ggccctcttg ctgctgcatg atctcggcgt
     1681 cgtgatcgcg cacggcgcgc gcgaccatgc gacggcggcg tggcgcgagg tatcgatcct
     1741 cgacccgaac tggctgaccc aggcgatcta ctcgctgctc accagccacg tggtccgcga
     1801 ccaacagggc gagctgcgcc gcgaccaact cggcgcgctg ctcgatcccg agcggtaccc
     1861 ggcgcgctgg cacgagctga tcctcagcat gatgcaggat cccgcgctcg gcctctgcct
     1921 gccgatcacg cgcggcgatg ccgagcgcta cctgctcccc gaggcattgc ctccctcgga
     1981 gcccgactac ggcatctggc ccgacgactc cctccgcttc cggttccagt accagcgcct
     2041 tcccgcgggc ctgctcccac gcctgatcgt ggaggcccac cgcagcctga ccgaccgacc
     2101 cacgtactgg cgcaccggcg tcgtgcttgc ttcggacgga tgcaggatcc tcgtgcgcgg
     2161 cgaggttggc aaggctcgcg tcgagataca cgtcgccggc cccacccacc ggcgcagcgc
     2221 gctcagcgtc gtacgcggct acttcgacgc ggtccatcgc cactacccca ggctcacggt
     2281 caacgcacga gtcccactcc ccgaacaacc cgaggtcgat gtcg
//
LOCUS       DASVOR010000074         3598 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_812,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000074 DASVOR010000000
VERSION     DASVOR010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_812"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF516_01660"
     CDS             complement(<1..407)
                     /locus_tag="VF516_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106396.1"
                     /translation="MILAPEEAPRIVDVDLQLQAAGLDPGTYHYRVSAVFSPGDPDNP
                     GGESLASDELTIRLPSFPGKKLVLTLIWRAPVDALGAALPNVTGYRIYRTAKDGAPGS
                     EVLLGTAPATPRTFVDDGARRPARPCRCRSAAPG"
     gene            683..1282
                     /locus_tag="VF516_01665"
     CDS             683..1282
                     /locus_tag="VF516_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106397.1"
                     /translation="MVTITSHVVLAGMPPPTVAVICVPSALTSTRDATTSTPGQCSAT
                     LAPGKKFAPSIVSCAPPWHTVDGTTLSTRGGTSIATSNAVLDAQSAGAGHHHLLIGLG
                     VADPHRPCRADHRGRQDREHLGQGRRVLAVRVGHGHVEHAGRQCIGRHDVEPHRRLVR
                     PVHAARRRILRIVGDLDRRRRQHPAGQRGAIAHQIRRDR"
     gene            1627..2250
                     /locus_tag="VF516_01670"
     CDS             1627..2250
                     /locus_tag="VF516_01670"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEX8106398.1"
                     /translation="MKIHALVVLAGCSWTGVQGSGNAKSEVRNVPAFSAVEVAGSFDA
                     EIAVGPESHVEITGDDNIVPLVTSDVHGDRLEIGTRKNVRPSVRLVARITVPRLTGIR
                     LTGSGDIAAHGVQADHLRVILSGSGTIRADGTAHEAEIDVTGSGTVALDKLAAERVRV
                     SVSGSGDVAVAASKALDVSITGSGDVTYRGDPELKKQISGSGNVTRR"
     gene            complement(2278..>3598)
                     /locus_tag="VF516_01675"
     CDS             complement(2278..>3598)
                     /locus_tag="VF516_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106399.1"
                     /translation="RWFLSRAHPIRDASGRIERWVGTNTDVTAQRFLDDATRILSGTL
                     NMGDALEQLAQLTVPDLADWCFVDLLEHGQLKHVAIAHGDEAKREAVRGFALRLSPDD
                     LPETSELGQILRSGTVAVLPEIADQGLAAYARSPEHLQLMRELAFKSWIGAPLIARGS
                     TIGVLHLLMSDSNRRYREADVELAVELGRRAGMAVDNARLYSEAQAAVRVRDDVLAIV
                     SHDLRSPLQAVDLGAAMLNQQCSADPGARRYLDTIRRSVDRMEHLINDLLDLASINAG
                     KLSTHPTRVVADELMDEIVDVYKPLAAERKITIVRGQELGDVALHVDRHRITQAFSNL
                     LGNAIKFCRPGDVVTVRGQRVGDGIQFTVADTGPGIAEADLPHLFEQYWSGRSGKTKG
                     AGLGLFITKAIIEAHHGHIAVSSQEGVGTMFVVTLPAAAEGATPYGA"
BASE COUNT          533 a         1295 c         1240 g          530 t
ORIGIN      
        1 cccggtgctg ccgagcggca gcggcacggc cgcgccgggc gtcgcgctcc gtcgtcgacg
       61 aacgtgcgcg gcgtcgccgg cgcggtgccg agcagcacct cgctgccggg cgcgccgtcc
      121 ttcgcggtgc ggtagatccg gtagccggtg acgttgggca gcgcggcgcc gagcgcgtcg
      181 accggcgcgc gccagatcag ggtgagcacg agcttcttgc cggggaagct cggcaaccgg
      241 atcgtgagct cgtcgctcgc cagcgactcg ccgccggggt tgtcgggatc gccgggcgag
      301 aacaccgcgc tgacccggta gtgatacgtg cccggatcga gccccgcggc ctggagctgg
      361 aggtcgacgt cgacgatccg gggcgcctcc tcgggcgcca ggatcatcgc gcgctcggcg
      421 ctcgcggtcg ggccggtgcc gtcgttgcca ccgaccaggt agatgtagcg tccgacggtg
      481 accgcgcctg ccagcgtgcg cggcgcccgc aggcggggcg gctggaccgc gaacgcgccg
      541 aacgtgccga acagatcgac cggcgcgacc tcgaccgagt cgagcgcgtt ggcgacggtg
      601 ccgtcgtcgc cgccgatcgc gtagacgaag cggttggccg cgggtcttgt gcggcagcgg
      661 caggtcggcg cacgagtccg cgttggtcac gatcacctcg cacgtggtgt tggccggcat
      721 gccgccgccc accgtcgccg tgatctgcgt gccatcggca ttgacgtcga cccgcgatgc
      781 caccacgtcc acccccgggc aatgcagcgc gaccttggcg ccgggcaaga agttcgcgcc
      841 gtcgatcgtg agctgcgcgc cgccctggca caccgtcgac ggcacgacgc tgtcgacccg
      901 cggcggcacg tcgatcgcga cctcgaacgc cgtgctcgac gcgcagtccg ccggcgccgg
      961 tcaccaccac cttctgatcg gcctcggcgt cgcagatccc catcggccgt gccgcgctga
     1021 ccaccggggg cggcaggatc gcgagcacct ggggcaaggt cgacgcgtgc ttgccgtccg
     1081 ggttggtcac ggtcacgtcg aacacgccgg tcggcaatgc atcggccggc acgacgtcga
     1141 accccatcgt cgactcgtcc gtccagtgca cgcggctcgc cgccggatcc ttcggatcgt
     1201 cggcgacctc gaccggcgac gccggcagca tcccgccggg cagcgcgggg ccattgcaca
     1261 ccagatccgg cgagaccgat gacaggctct cggtggggct cgtcacctcg tgcgagcacg
     1321 cggcgagcga gcacacgaca acagcgcata agcgattggc agacatgatt ctccgtttcc
     1381 gcaccgcagc aaccgcggtg ccagcaaccg cgtcatcgcg atcgctagtt gcgctcgccg
     1441 cgccttcgcc gatcgtcccg atccgtacac cgcgtcccga tccgaccatg cacagcgcga
     1501 caccacgacg acgccggcgc catcgtggcg atctgcctcg ttgctgcgaa tccgcaccgc
     1561 gccccccgcc gggatttgcc atgctcgtga attgtcggat tcgctcccac cagctagctt
     1621 cgccgcatga agattcatgc gcttgtcgtg ctcgcggggt gcagctggac cggagtccag
     1681 ggaagtggca acgccaagag cgaggtccgc aacgtgccgg cgttcagcgc ggtcgaggtc
     1741 gccggctcat tcgacgccga gatcgcggtc ggccccgaat cccatgtcga gatcaccggc
     1801 gacgacaaca tcgtcccgct ggtcaccagc gacgtgcacg gcgatcggct ggagatcggc
     1861 acgcgcaaga acgtgcggcc gagcgtgcgc ctggtcgccc ggatcaccgt gccgcggctc
     1921 accggcatca gactgaccgg ctcgggggac atcgccgccc acggcgtcca ggccgaccac
     1981 ctgcgcgtca tcctgtccgg atcggggacg atccgcgccg acggcacggc gcacgaggcc
     2041 gagatcgacg tgaccggcag cggcacggtc gcgctcgaca agctcgccgc cgagcgggtc
     2101 cgcgtctcgg tgtcgggcag cggcgacgtc gcggtcgcgg cgtccaaggc gctcgacgtc
     2161 tcgatcaccg gctcgggcga cgtcacctac cgcggcgatc cggagctcaa gaagcagatc
     2221 agcggctccg gcaacgtgac ccggcgctga ccgcgcgcgg cgccgcgaag cagctgctca
     2281 agcgccgtac ggcgttgcgc cttcggctgc ggccggcagc gtcacgacga acatcgtgcc
     2341 gacgccctcc tgggaggaga ccgcgatgtg accgtggtgc gcttcgatga tggccttggt
     2401 gatgaacagc cccagcccgg cgcccttggt cttcccgctg cgacccgacc agtactgctc
     2461 gaagaggtgc ggcaggtcgg cctccgcgat ccccggcccg gtatcggcca ccgtgaactg
     2521 gatgccgtcg ccgacccgct gaccgcgcac cgtgacgacg tcgccgggcc tgcagaactt
     2581 gatcgcattg ccgagcaagt tgctgaatgc ctgcgtgatc cgatgccgat cgacgtggag
     2641 cgcgacgtcg ccgagctcct gtccgcgcac gatggtgatc ttccgctcgg cggccagcgg
     2701 cttgtacacg tcgacgatct cgtccatcag ctcgtcggcc accacacgcg tcgggtgagt
     2761 cgagagcttg cccgcgttga tgctcgccag gtcgagcagg tcgttgatca ggtgctccat
     2821 ccgatccacg gaccgccgga tcgtgtcgag gtacctccgc gcgcccggat ctgcgctgca
     2881 ctgctggttc agcatcgccg cgccgagatc cacggcttgc agcggagagc gcaggtcgtg
     2941 cgatacgatc gcgagcacgt cgtcgcggac gcggaccgcc gcctgcgcct cgctgtacag
     3001 ccgcgcattg tccaccgcca tccccgcgcg ccggcccagc tcgacggcga gctcgacatc
     3061 cgcttcgcga taccgccggt tcgagtcgct catgagcagg tggagcacgc cgatcgtgct
     3121 cccccgagcg atcagcggcg caccgatcca ggacttgaag gcgagctcgc gcatgagctg
     3181 gaggtgctcc gggctccgcg cgtacgcggc gagcccttga tcggcgatct cgggcagcac
     3241 ggccacggtg ccggaacgca agatctgccc cagctcactg gtctccggca gatcgtcggg
     3301 tgacaagcga agcgcgaacc cgcgcaccgc ttcacgcttc gcctcgtcgc catgcgcgat
     3361 cgccacgtgc ttgagctggc cgtgctcgag cagatcaacg aagcaccagt ccgccaggtc
     3421 gggcacggtg agctgcgcga gctgttcgag cgcatctccc atgttcaggg tgccgctgag
     3481 gatgcgggtc gcgtcatcga ggaagcgctg cgcggtgacg tcggtgttgg tcccgaccca
     3541 gcgctcgatc ctgccggagg cgtcgcggat cgggtgcgcg cgcgacagga accagcgg
//
LOCUS       DASVOR010000075         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_234532,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000075 DASVOR010000000
VERSION     DASVOR010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234532"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1301)
                     /locus_tag="VF516_01680"
     CDS             complement(<1..1301)
                     /locus_tag="VF516_01680"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8106400.1"
                     /translation="MRDDFPRAAVGASLVVWLGLCAAVHADPADRPSDPPVHAGTQPT
                     PPVRPGEPVLPQELDGRRAIRGCSVDERCARPGDLLREFVVEAFPPPGSSPWLDERTP
                     PPSRLEPGPPRPIVKRPSELRPDAPWLDQLELPDLPVRWSQTLVDYLVFYRDDPRGRS
                     IMQSWIVAQGRYRDLIVGHLRRAHLPEDLLYVAMIESGYDPGDTSPAGALGIWQFMPE
                     GGRIYGLREDRWVDERRDPLRSTIAQMDYFADLHQRFADWNIALAAFNMGYGAMLRSI
                     ARYNTNDYYRLCEYENAIPWETCLYTPKVLAAAIVGHNRALYGFDKLTTAPAEAWDEV
                     AIPSAASLAAIARIAGVAEADLKRLNPQLRHGRTPPGEAGYVVRVPRGARSEAQRRLV
                     ELESDWKNYDAYVIAHGERFEDVATTFGVTVAQLRKLNDVTR"
     gene            1420..1710
                     /gene="tatA"
                     /locus_tag="VF516_01685"
     CDS             1420..1710
                     /gene="tatA"
                     /locus_tag="VF516_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEX8106401.1"
                     /translation="MPGMGELIVILLIVLVVFGANKLPGIGDALGRSIKNFKKASNVD
                     EDDGDKKDDRAAKKELASAKSSKQLEEGSGAEDGEWEEVVVRRRKKPAASGE"
     gene            complement(1718..2353)
                     /locus_tag="VF516_01690"
     CDS             complement(1718..2353)
                     /locus_tag="VF516_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106402.1"
                     /translation="MTRTHIHDDPDAAEALEAITHAAADPALPYRTRQRLYEAATSQQ
                     LSAIARLFLVASPVTVGADQLARSLAPERPLRPTGRLLTLGERKALARTHDRQQLLLL
                     LRDPHPAVIAILLDNPHVTEDDIVRIAAARPAVPASLALVAAHARWSVRHAVKRALVF
                     NPSTPLADAIRIATTLRFQELRALAADESLPEPLRTHAAEVYAAAMRRPQA"
     gene            2566..3198
                     /locus_tag="VF516_01695"
     CDS             2566..3198
                     /locus_tag="VF516_01695"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HEX8106403.1"
                     /translation="MPETRIIKRYANRKLYDTEHSRYVTLDQISEMIRNGDDVKIVDN
                     KTKEDLTTVTLAQIIFEEEKKQRSFLPLGAMRNIIQSGGEWFAEAQRRVQSILPGKRK
                     DEAAPEPAGDDPPAEPADETALKKRSLASLREWVDHSKHRLDEWQKQVDTKIRTTIDG
                     ISQTINPWASVNKDVRALADRIDELETKLRELESHEERGTTTTTTTDRSG"
     gene            complement(3326..>3549)
                     /locus_tag="VF516_01700"
     CDS             complement(3326..>3549)
                     /locus_tag="VF516_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106404.1"
                     /translation="PPPEHPVPDKTDRMPVEDTGKPAPVERPAPARRPAERRPARQPP
                     PPADDDLGLGKLQGGVKPGDKKPADAKKH"
BASE COUNT          573 a         1153 c         1323 g          500 t
ORIGIN      
        1 cgggtgacgt cgttgagctt gcgcagctgg gccacggtga ccccgaacgt ggtcgcgacg
       61 tcctcgaacc gctcgccgtg ggcgatcacg taggcgtcgt agttcttcca gtccgactcg
      121 agctcgacca ggcggcgctg cgcctcggac cgggcgccgc gcgggacgcg cacgacgtag
      181 ccggcctcgc cgggcggggt gcggccgtgg cggagctgcg ggttgagccg cttgaggtcg
      241 gcctcggcga cgccggcgat ccgcgcgatc gccgcgagcg acgccgcgct cgggatcgcg
      301 acctcgtccc aggcctcggc cggggcagtg gtgagcttgt cgaagccgta cagcgcgcgg
      361 ttatggccga cgatcgcggc ggcgagcacc ttgggcgtgt acaggcaggt ctcccacggg
      421 atcgcgttct cgtactcgca cagccggtag tagtcgttgg tgttgtagcg cgcgatcgag
      481 cgcagcatcg cgccgtagcc catgttgaac gcggcgagcg cgatgttcca gtcggcgaac
      541 cgctggtgga ggtcggcgaa gtagtccatc tgggcgatcg tcgagcgcag cggatcgcgg
      601 cgctcgtcga cccagcggtc ctcgcgcagc ccgtagatcc ggccgccctc gggcatgaac
      661 tgccagatcc cgagcgcgcc ggccggcgag gtgtcgcccg ggtcgtagcc cgactcgatc
      721 atcgcgacgt acagcagatc ttccgggagg tgggcccggc gcaggtggcc gacgatcagg
      781 tcgcggtagc ggccctgcgc gacgatccag ctctgcatga tgctgcggcc gcgcgggtcg
      841 tccctgtaga acacgaggta gtcgaccagg gtctgcgacc accggaccgg cagatcgggg
      901 agctcgagct gatcgagcca cggcgcgtcg ggccggagct cgctcggccg cttgacgatc
      961 ggccgcggcg ggcccggctc gagccggctc ggcggcggcg tgcgctcgtc gagccacggg
     1021 ctggaccccg gcggcgggaa cgcctcgacc acgaactcgc gcagcaggtc gccgggccgc
     1081 gcgcagcgct cgtcgaccga gcagccgcgg atcgcgcgcc ggccatcgag ctcctggggc
     1141 agcaccggct cacccggccg gaccgggggc gtcggctggg tgcccgcatg gaccggggga
     1201 tcggacggcc gatcggccgg atccgcgtgg accgccgcgc acaggccgag ccagaccacg
     1261 aggctggctc cgacggccgc gcgggggaaa tcatcgcgca tatcccgaaa gataggcggc
     1321 gggatgaggc ttcaccaagt tttcagggcc ggttttgccc cgagggcgct tcggtgcgca
     1381 aacagtgcta gtctgccgcc agtcatctag gagtgcccca tgcccggaat gggcgagctg
     1441 atcgtcatcc tgctcatcgt cctcgtggtc ttcggtgcca acaagctgcc cggcatcggc
     1501 gacgcgctgg gccgaagcat caagaacttc aagaaggcgt cgaacgtcga cgaggacgac
     1561 ggcgacaaga aggacgaccg ggcggcgaag aaggagctgg cgtccgccaa gtcctccaag
     1621 cagctggagg aggggagcgg cgcggaggat ggcgagtggg aagaagtcgt ggtgcgccgg
     1681 cgcaagaagc cggccgccag cggggagtga ccggctgtca ggcttgcggg cggcgcatcg
     1741 cggccgcgta gacctcggca gcgtgggtgc gcagcggctc gggcagtgac tcgtcggccg
     1801 ccagcgcgcg gagctcctgg aaccgcaggg tcgtcgcgat ccggatcgca tcggcgagcg
     1861 gggtcgacgg gttgaacacc agcgcgcgct tgaccgcgtg gcggaccgac caccgcgcgt
     1921 gcgcggcgac cagggcgagc gacgccggga ccgcgggccg cgcagccgcg atccggacga
     1981 tgtcgtcctc ggtgacgtgc gggttgtcga gcaggatcgc gatcaccgcc gggtggggat
     2041 cgcgcagcag gagcagcagt tgctggcggt cgtgcgtccg ggccagggcc ttgcgctccc
     2101 cgagcgtgag cagccgaccg gtcggtctga gcggccgctc cggtgccagc gagcgggcga
     2161 gctgatcggc gcccaccgtc accggcgacg cgaccaggaa cagccgggcg atcgccgaca
     2221 actgctgaga agttgcggcc tcgtacagac gttggcgggt gcggtagggc agcgccgggt
     2281 cggccgcggc gtgggtgatc gcctccagcg cctcggccgc atcggggtcg tcgtggatgt
     2341 gggtgcgggt catgatccct gcgatagcct gcacccaggt cgttggatct ccctcacaga
     2401 aggcctcgat cagggcaagc gtgcgcatcc gcgggtctcg taatgcccgc aggcgagcca
     2461 ggaggtctat ccccacacga atagtgtggc acgttgagga catggcgcgc tgcgtgatac
     2521 tttttggaat tatcgagccg tcttttgcgc ggcggtaggt gcgcgatgcc cgagacccgg
     2581 atcatcaagc gatatgcgaa ccgaaagctg tacgacaccg agcacagcag atatgtgacg
     2641 ctcgaccaaa tcagcgagat gattcgcaat ggcgacgacg tcaagatcgt cgacaacaag
     2701 accaaagaag acctgaccac ggtcacattg gcgcagatca tcttcgagga ggaaaagaag
     2761 cagcgcagct tcctcccgct tggcgcgatg cgcaacatca tccagagtgg cggtgagtgg
     2821 ttcgccgagg cgcagcgccg ggtccagagc atcctgcccg gtaagcgtaa agatgaagca
     2881 gcgccggaac ccgccggtga cgatccgccc gccgagccgg ccgacgagac cgcgctcaag
     2941 aagcgcagcc tcgcgtcgct ccgcgagtgg gtcgatcact ccaagcaccg gctcgacgag
     3001 tggcagaagc aggtcgacac caagatccgg acgacgatcg acgggatctc gcagacgatc
     3061 aacccgtggg cgagcgtcaa caaggacgtg cgcgcgctcg ccgatcgcat cgacgagctc
     3121 gagaccaagc tgcgcgagct cgagagccac gaggagcgcg gaacgacgac aacgacgacg
     3181 acggaccgct cgggctgacg tcgatcgttc ggccgatcga gatcggcctt cggcagtcga
     3241 agcacggcca cgacgcccat ccgcgcagcg gcccacggcg agccgagcgt gcgcgcgttc
     3301 tccgggcgct atgccctaat gcaccctagt gcttcttggc gtcggccggc ttcttgtcgc
     3361 cgggcttgac gccaccctgc agcttgccga ggccgaggtc gtcatcggcc ggcggcggtg
     3421 gctgcctggc gggccggcgc tcggcgggtc ggcgcgccgg cgcgggtcgc tcgacgggtg
     3481 cgggcttgcc ggtgtcctcg accggcatgc gatcggtctt gtcggggacc gggtgctcgg
     3541 gtggcgggg
//
LOCUS       DASVOR010000076         2742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_351536,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000076 DASVOR010000000
VERSION     DASVOR010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351536"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2324
                     /locus_tag="VF516_01705"
     CDS             <1..2324
                     /locus_tag="VF516_01705"
                     /inference="COORDINATES: protein motif:HMM:NF021355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-propeller domain-containing protein"
                     /protein_id="HEX8106405.1"
                     /translation="GGVGVGSVATGPSVVVSSVSFANGRIAQVDHVQFDGFAGVFNVT
                     PNAIMLASDASTWTGGPPGTPGTYTAKTRMLYLDISDPGGAIVQRGALTVDGRINGWG
                     ADNGRWNLDFADGKTAHVVGQASDTTSGYVLSTVDFSNPGAPALMSAFQIANTGWSAA
                     ARFDTSRLYLSPATGYYNGSATTPLQVYDLTDPRAPRLAGTAQLPGMVWNILPAPSSR
                     IFALGNGTQLASEGDPVSVHYLDATDPAAPKLLGTSTFGQGWAWTPAAGTFKAFTMDV
                     ASGLVVVPFAGWDPAGRGYNNGLQLIEFTPSSVTTAGAARTKGWVERGIFVGNRLVSL
                     SPQSLAVVDYTAPMAPRVVSELTLARNVVTAQPSGQTIAEVSSDWWGNDTSSSEVRVL
                     PIGNAEETTDAAGVPTVQVDGVGAQVFTNGRYTYIVTSVPTDRSCGGYGFGCGTRTQQ
                     VQVVDLAGGGAVLRGKLQLPVDAWGGFGGWEGFWWWDWYWGSEVVQVGGDVLAFRRWE
                     PRFSGSGAYLDTQTRLLVVDLANPDAPQLASVVVQTDPQAWWGNLKVVGDTLYTTHYE
                     WVEAPDFQRGWVRYYLDSIDLGDRAHPQIGARVNVPGLLVGGDANDPSVIYTIDYRWN
                     DGTSVNDLDVLRIQGSKARLLSQTPISGWVGSTFIVGTQAYLSAQEYNRNGTGSVDLH
                     AIDLSNPSHPRDRIASEHGWGWLLAVAGDRALVTSGWSGEALDVYRLRAGGAPQFEQT
                     VRTRGWGVNGVSRQDQTLFLSTGYWGVQKIALP"
     gene            2371..>2742
                     /locus_tag="VF516_01710"
     CDS             2371..>2742
                     /locus_tag="VF516_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106406.1"
                     /translation="MIDDLLAFLRASPTPFHAVDQARRRLEAHGFRALDEAEPWDQLA
                     PGAYTVTSSGSNLFACVLPEARDHRRRFCLVGAHTDSPNLRIKPNAEYRSEGYAQVGV
                     EVYGGALLNSWLDRDLGVAGRV"
BASE COUNT          378 a          935 c         1053 g          376 t
ORIGIN      
        1 gcgggggcgt gggggtgggc agcgtggcga ccgggccgtc ggtggtcgtg tcgtcggtga
       61 gcttcgcgaa cggccggatc gcgcaggtcg accacgtgca gttcgacggg ttcgcgggcg
      121 tgttcaacgt gacgccgaac gcgatcatgc tggcgtcgga cgcgtcgacc tggaccggcg
      181 ggccgcccgg cacgcccggc acgtacaccg cgaagacccg gatgctgtac ctcgacatct
      241 cggatccggg cggcgcgatc gtgcagcgcg gcgcgctcac ggtcgacggc cggatcaacg
      301 gctggggcgc cgacaacggg cgctggaacc tcgacttcgc cgacggcaag acggcccacg
      361 tggtgggcca ggccagcgac accaccagcg gctacgtgct gtcgacggtc gacttctcga
      421 acccgggcgc gcccgcgctg atgtcggcgt tccagatcgc gaacaccggg tggagcgcgg
      481 cggcgcggtt cgacaccagc cggctctacc tgtcgcccgc gaccggctat tacaatggca
      541 gtgccacgac gccgctccag gtctacgacc tgaccgaccc cagggcgccg cggctggcgg
      601 gcaccgcgca gctgccgggc atggtgtgga acatcctgcc ggcgccgagc tcgcggatct
      661 tcgcgctcgg caacggcacg cagctcgcga gcgagggcga tccggtgtcg gtgcactacc
      721 tcgacgccac cgacccggcg gcgccgaagc tgctcggcac gtcgacattc ggccagggct
      781 gggcgtggac gccggcggcc ggcacgttca aggcgttcac gatggacgtc gcgagcgggc
      841 tggtggtggt gccgttcgcg ggctgggatc cggcggggcg cggctacaac aacgggctgc
      901 agctgatcga gttcacgccg agcagcgtga cgaccgccgg cgcggcgcgc accaagggct
      961 gggtcgagcg cgggatcttc gtcggcaacc ggctggtgtc gctgtcgccg cagtcgctcg
     1021 cggtggtcga ctacaccgcg ccgatggcgc cgcgcgtggt cagcgagctg acgctggcgc
     1081 gcaacgtggt gaccgcgcag ccgagcgggc agacgatcgc cgaggtgtcg tcggactggt
     1141 ggggcaacga cacgtcgagc tccgaggtcc gcgtgctgcc gatcggcaac gccgaggaga
     1201 ccaccgacgc ggccggggtg cccacggtcc aggtcgacgg cgtcggcgcg caggtgttca
     1261 ccaacggccg ctacacgtac atcgtgacca gcgtgccgac cgacaggtcc tgcggcggct
     1321 acggcttcgg ctgcggcacg cgcacgcagc aggtccaggt ggtcgacctc gccggcggcg
     1381 gggcggtgct gcgcggcaag ctccagctgc cggtggacgc gtggggcggg ttcggcggct
     1441 gggaggggtt ctggtggtgg gactggtact ggggcagcga ggtcgtgcag gtcggcggcg
     1501 acgtgctggc gttccgccgc tgggagccgc ggttcagcgg cagcggcgcg tacctcgaca
     1561 cccagacccg gctgctggtc gtcgatctcg cgaacccgga tgcgccgcag ctggcgtcgg
     1621 tcgtggtcca gaccgatccg caggcctggt ggggcaacct gaaggtcgtc ggcgacacgc
     1681 tgtacacgac gcactacgag tgggtcgagg cgccggactt ccagcgcggc tgggtccgct
     1741 actacctcga ctcgatcgac ctcggcgacc gcgcgcaccc gcagatcggc gcccgggtca
     1801 acgtgccggg cctgctggtc ggcggcgacg ccaacgatcc gagcgtgatc tacacgatcg
     1861 actatcgctg gaacgacggg acgtcggtca acgacctcga cgtgctgcgg atccagggct
     1921 cgaaggcgag gctgctgtcg cagacgccga tcagcggctg ggtcggctcg acgttcatcg
     1981 tcggcacgca ggcctacctg tcggcgcagg agtacaaccg gaacggcacc gggtcggtcg
     2041 acctgcacgc gatcgacctg tcgaacccgt cgcacccgcg cgaccggatc gcgagcgagc
     2101 acggctgggg ctggctgctc gcggtggccg gcgaccgcgc gctggtgacg tcgggctggt
     2161 cgggcgaggc gctcgacgtc taccggctgc gcgccggggg ggcgccgcag ttcgagcaga
     2221 cggtgcggac ccgcggctgg ggggtcaacg gcgtgtcgcg gcaggaccag acgctgttcc
     2281 tgtcgaccgg ctactggggg gtgcagaaga tcgcgctgcc gtgaccccgg cggcgggccg
     2341 cgcggcgcgg cggtggtagc ctcgcgccgc atgatcgacg atctgctggc cttcctgcga
     2401 gcttctccca cgccgttcca cgcggtcgac caggcccgcc gccggctcga ggcgcacggg
     2461 ttccgcgcgc tcgacgaggc cgagccgtgg gatcagctcg cgccgggcgc gtacacggtg
     2521 acctcgagcg gcagcaacct gttcgcgtgc gtgctgcccg aggcgcgcga ccaccgccgc
     2581 cggttctgcc tggtcggcgc ccacaccgac agcccgaacc tgcggatcaa gccgaacgcg
     2641 gagtacagga gcgagggcta cgcgcaggtc ggcgtcgagg tctacggcgg cgcgctgctc
     2701 aactcgtggc tcgatcgcga cctcggcgtc gccggccgcg tg
//
LOCUS       DASVOR010000077         4365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_878,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000077 DASVOR010000000
VERSION     DASVOR010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4365
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_878"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..3403
                     /locus_tag="VF516_01715"
     CDS             95..3403
                     /locus_tag="VF516_01715"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HEX8106407.1"
                     /translation="MSQDEELHAMDADRSEPGSEPGSEPGAEPDGDDHSEHHDDEHDA
                     APLADLAPTPVHGYWKSLRELEGKAAFQVGRTGHEFPAGADQPPKDPLSRRNFFQLMG
                     ASMALAGVGACRRYEKEEIVPLARRPEDQVPGMTQQYATAYELGGVGHALLATSFEGR
                     PIKLDGNPEHPFASGGVVPGTERRAGCSAFVQAAILHLYDPDRSQNPTADHGTGSSMD
                     AFRAALPQLRAALAQTVILSEASSSPTLRALRTRLLGQGVRWFEYEPLSWDNERAGTR
                     LAFGRPLRPIARLDRAETIVALDCDLFLEHPAAMKYSRDFARSRRQNGSLGIGKMNRL
                     WSIESVFTNTGAMADHRLALRAELGLPLAVALDARLSGGTAPAAEFLQEPKVDKFLRF
                     LVEELKANRGKAVVIAGRRQPPEVHAVVARINQALGAPGQTLDYVEDPDGDRPSHLRA
                     ITELTRAMTAGQVGTLIMLGGNPVYDAPADLDFAAALGKVKTSIHLHEYRNETSLAAS
                     WHVPRAHFLEAWGDVRTWDGTITLAQPLIAPLYGGLSAIELLSLLLGDEKGGEELVRG
                     VHKELGFEAGWKQNVHDGFVPDTQLAAVAATPVAFQVAPLTAGQQSGSLRTNGDLEVV
                     FVQSSFTYDGRFANNAWLWETPDFMTKVTWDNYALVGASTALGLGLENDTLIKVKIGD
                     GEHGREIELPCYTIPGQAKGSIGLVLGGGRTAAGRVGGNGHRRVGWDTYKVRTTTGWD
                     FASKASVAPTGKPYTLANIQEHWDIRTGIGGRPEISRDAAHVNAKGLDERLPELIRET
                     TQRTLNEDRKYRAEEPEGFFNDLANAGNTDRPNEGGYSLFKEKDYQGHRWAMAIDLST
                     CTGCNACAVACQAENNIPVVGRNEVMNNREMSWIRIDRYFSGSIEDPQVVHQPIACQQ
                     CEQAPCEQVCPVGATSHSDEGLNDMAYNRCIGTRYCANNCPYKVRRFNFLDWNKEWRD
                     ARNQVRRLLFNPEVTVRMRGVMEKCTFCVQRIQNAKIRAKAQIRSGQRPGSVTAVLPD
                     GELETACQQACPTEAIVFGDLADKDSRVARLHEDRRSYALLPETYTKPRNRFLARVRN
                     PNPDFAAALQGGHE"
     gene            3421..>4365
                     /gene="nrfD"
                     /locus_tag="VF516_01720"
     CDS             3421..>4365
                     /gene="nrfD"
                     /locus_tag="VF516_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX8106408.1"
                     /translation="MDSHHAEAGKAREAFRAVTRDVAWVAEAPRPHKAWLAALAVSVT
                     ALAIGLYSIYVLVTTGIGVWGNSNTVCWAWDITNFVWWIGIGHAGTLISAVLYLTRQT
                     WRVSINRSAEAMTIFAVMCAGLFPLLHTGRPWFAWWMIPHPNDMGMWPQFRSPLMWDV
                     FAVTTYMLTSILFWYMGMVPDLATFRDRCAGRGDKLRAIIYGALSLGWRGSARQWHRY
                     ERAYLILAGLATPLVFSVHSVVSFDFATSQLPGWHTTIFPPYFVAGAIFSGFAMVFTL
                     LIPARHLFNLKHIIKDEHVEAACKIMLATGMMVGLAYAS"
BASE COUNT          696 a         1592 c         1482 g          595 t
ORIGIN      
        1 tcagccccaa ccgggcgagg gcgaggacgt ccgcaagaag aacggtgtca acccgcccac
       61 caactgctcg acctgccatc gctaggaccc gacgatgtcc caagacgaag agcttcacgc
      121 catggatgca gatcgatccg aaccgggctc cgaaccgggc tccgaaccgg gcgcggaacc
      181 tgacggcgat gaccacagcg agcaccacga cgacgagcac gacgccgcgc cgctggcgga
      241 cctggccccg acgccggtcc acggctactg gaagtcgctg cgcgagctcg agggcaaggc
      301 ggcgttccag gtcggacgca ccggccacga gttccccgcc ggcgcggatc agccgccgaa
      361 ggatccgctg tcgcgccgca acttcttcca gctgatgggc gcgtcgatgg cgctcgccgg
      421 cgtcggcgca tgccggcgct acgagaagga ggagatcgtc ccgctcgccc ggcgccccga
      481 ggatcaggtc ccgggcatga cccagcagta cgccacggcg tacgagctcg gcggcgtcgg
      541 ccacgcgctg ctcgcgacgt cgttcgaggg ccggccgatc aagctcgacg gcaaccccga
      601 gcacccgttc gccagcggcg gcgtggtgcc cggcaccgag cgccgcgccg ggtgctcggc
      661 gttcgtccag gccgcgatcc tccacctcta cgacccggac cgctcgcaga acccgaccgc
      721 ggatcacggc accggctcgt cgatggacgc gttccgcgcc gcgctgcccc agctgcgcgc
      781 ggccctggcg cagacggtca tcctcagcga ggcgtcgtcg tcgccgaccc tgcgcgcgct
      841 gcgcacccgg ctgctcggcc agggcgtccg gtggttcgag tacgagccgc tgtcgtggga
      901 caacgagcgc gccgggaccc ggctcgcgtt cggccgcccg ctgcgcccga tcgcccggct
      961 cgaccgcgcc gagaccatcg tcgcgctcga ctgcgacctg ttcctcgagc acccggccgc
     1021 gatgaagtac agccgcgact tcgcgcggtc gcggcgccag aacggctcgc tggggatcgg
     1081 caagatgaac cggctgtggt cgatcgagag cgtgttcacc aacaccggcg cgatggccga
     1141 ccaccggctc gcgctgcgcg ccgagctcgg cctgccgctg gcggtcgcgc tcgacgcccg
     1201 gctgtccggc ggcaccgcgc cggccgccga gttcctccag gagcccaagg tcgacaagtt
     1261 cctgcggttc ctcgtcgagg agctcaaggc caaccgcggc aaggcggtcg tgatcgccgg
     1321 ccggcgccag ccgcccgagg tccacgcggt ggtcgcgcgg atcaaccagg cgctcggcgc
     1381 tcccggccag accctggact atgtcgagga tcccgacggc gaccggccga gccacctgcg
     1441 cgcgatcacc gagctcaccc gggcgatgac cgcgggccag gtcggcacgc tgatcatgct
     1501 cggcggcaac ccggtgtacg acgcgccggc cgatctcgac ttcgccgcgg cgctcggcaa
     1561 ggtcaagacc tcgatccacc tccacgagta ccgcaacgag acctcgctgg cggcgagctg
     1621 gcacgtgccg cgcgcgcact tcctcgaggc gtggggcgac gtgcggacct gggacggcac
     1681 gatcacgctg gcccagccgc tgatcgcgcc gctctacggc gggctgtcgg cgatcgagct
     1741 gctgtcgctc ttgctcggcg acgagaaggg cggcgaggag ctggtccgcg gcgtccacaa
     1801 ggagctcggc ttcgaggccg gctggaagca gaacgtgcac gacggcttcg tgcccgacac
     1861 ccagctcgcc gccgtcgcgg cgaccccggt cgcgttccag gtcgccccgc tgaccgccgg
     1921 ccagcagtcc ggctcgctgc gcaccaacgg cgacctcgag gtcgtgttcg tgcagtcgag
     1981 cttcacctac gacgggcggt tcgccaacaa cgcctggctg tgggagacgc ccgacttcat
     2041 gaccaaggtg acctgggaca actacgcgct ggtcggcgcg agcaccgcgc tcggtctcgg
     2101 gctcgagaac gacacgctga tcaaggtcaa gatcggcgat ggcgagcacg gaagggagat
     2161 cgagctgccc tgctacacga tccccggcca ggccaagggc tcgatcggcc tggtgctcgg
     2221 cggcgggcgc accgcggccg gccgggtcgg cggcaacggg caccgccggg tcggctggga
     2281 cacgtacaag gtccgcacca ccaccggctg ggacttcgcg agcaaggcca gcgtcgcgcc
     2341 caccggcaag ccgtacacgc tggccaacat ccaggagcac tgggacatcc gcaccgggat
     2401 cggcggccgg cccgagatca gccgcgacgc ggcccacgtc aacgccaagg ggctcgacga
     2461 gcggctgccc gagctgatcc gcgagaccac ccagcgcacg ctcaacgagg acaggaagta
     2521 tcgcgccgag gagcccgagg ggttcttcaa cgacctcgcc aacgccggca acaccgatcg
     2581 cccgaacgag ggcggctact cgctgttcaa ggagaaggac taccagggcc accgctgggc
     2641 gatggcgatc gacctgtcga cctgcaccgg ctgcaacgca tgcgcggtcg cctgccaggc
     2701 cgagaacaac atcccggtgg tcggccgcaa cgaggtgatg aacaaccgcg agatgtcgtg
     2761 gatccggatc gaccgctact tcagcggctc gatcgaggac ccgcaggtcg tgcaccagcc
     2821 gatcgcctgc cagcagtgcg agcaggcgcc gtgcgagcag gtctgcccgg tcggcgcgac
     2881 cagccactcc gacgagggcc tcaacgacat ggcgtacaac cgctgcatcg gcacgcggta
     2941 ctgcgccaac aactgcccgt acaaggtgcg gcggttcaac ttcctcgact ggaacaagga
     3001 gtggcgcgac gcccgcaacc aggtccgccg cctcctgttc aatcccgagg tcacggtccg
     3061 gatgcgcggc gtgatggaga agtgcacgtt ctgcgtccag cggatccaga acgcgaagat
     3121 ccgcgccaag gcgcagatcc ggtccggcca gcgcccgggc agcgtcacgg ccgtgctgcc
     3181 cgacggcgag ctcgagaccg cgtgccagca ggcgtgcccg accgaggcga tcgtgttcgg
     3241 cgatctcgcc gacaaggaca gccgggtcgc ccggctccac gaggatcgcc gcagctacgc
     3301 gctgttgccc gagacctata ccaagccgcg caaccgcttc ctcgcgcggg tccgcaaccc
     3361 gaaccctgac ttcgccgccg ccctgcaggg gggtcacgaa tgagcaccgc agacgcaacc
     3421 atggactccc accacgccga ggccggcaag gcccgcgagg cgttccgcgc ggtcacgcgc
     3481 gacgtcgcgt gggtcgccga ggcgccgcgg ccgcacaagg cgtggctcgc cgcgctcgcg
     3541 gtctcggtga cggcgctcgc gatcggcctg tactcgatct acgtgctggt cacgaccggc
     3601 atcggcgtgt ggggcaactc gaacacggtg tgctgggcgt gggacatcac caacttcgtg
     3661 tggtggatcg gcatcggtca cgccggcacg ctgatctcgg cggtgctcta cctgacgcgc
     3721 cagacctggc gggtcagcat caaccgctcc gccgaggcaa tgacgatctt cgccgtgatg
     3781 tgcgccggcc tgttcccgct gctccacacc ggccggccgt ggttcgcgtg gtggatgatc
     3841 ccgcacccca acgacatggg gatgtggccg cagttccgca gcccgctgat gtgggacgtg
     3901 ttcgcggtca ccacgtacat gctgacgtcg atcctgttct ggtacatggg catggtcccg
     3961 gacctggcga cgttccgcga ccgctgcgcc gggcgcggcg acaagctgcg cgcgatcatc
     4021 tacggcgcgc tgtcgctggg ctggcgcggc tcggctcgcc agtggcaccg ctacgagcgc
     4081 gcgtacctga tcctcgccgg cctggcgact ccgctggtgt tctcggtcca ctcggtcgtg
     4141 tcgttcgact tcgcgacctc gcagctgccg ggctggcaca ccacgatctt tccgccgtac
     4201 ttcgtcgccg gcgcgatctt ctcggggttc gcgatggtgt tcacgctgct gatcccggcg
     4261 cgccacctgt tcaacctcaa gcacatcatc aaggacgagc acgtcgaggc cgcctgcaag
     4321 atcatgctgg cgaccggcat gatggtcggc ctggcctacg ccagc
//
LOCUS       DASVOR010000078         3075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410014,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000078 DASVOR010000000
VERSION     DASVOR010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410014"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2715
                     /locus_tag="VF516_01725"
     CDS             <1..2715
                     /locus_tag="VF516_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106409.1"
                     /translation="FTQSIGNLPASATLIARITIDLRLTWLPEGEWELRFPTVIGPRY
                     VAAADRPEDVRGTEIVTVPDGVAARIRIAIAIDDTLGAGRKPTSPSHALTIGDDGVVE
                     LADPAGARLDRDLVLRWPVTGPTTGLSLAMMRPEQGDECFGLVTIVPPAPATVTRTFA
                     RDLIVLIDTSGSMGGRPLDKAKQVIALLIESLGEEDGLELIAFSSQPRRYKPAAVQAT
                     RDEKQAAIRWLRALSAGGGTEMRIAVFEALQRLRPGTQRQVVIATDGYIADDTRLVSA
                     LLIHLPAGGRLHVVGIGSSVNRALTRALSRVGRGAEVICDLDGDPERAVKRLIDCTRW
                     PVAIDLALEGSALVQRAPWAIPDVFSGSPVVVAVKLQAGDLVVRGQAIDGEWVCRLRV
                     SLPRRGDGSPGIAALYGRERVADHEARCFAGERRDAEIERLGITHQITTRYTAFVAID
                     EALTTRPGARVEVVPQEIPYGTSAAAFGLSSPDASLVSDRIAVGRALLRGLAPADMMF
                     GSAAGAAPLSAGPSVTGGRILVENDIDEIHETTTRITDTRRPGDTSSEVPKLEGFMAD
                     IPGEPPATSAEPVQGPGDTFDAGGIDTSGPTVTAGPVLPVGGRIHQYETIRILGHGGM
                     SSVFLALDLRLVRLVAIKFLPSSDPELTQRFLVEARATARCQHENIVSIHEVGEHDGA
                     PYMVLEYLRGEPLRRLIENRQKLPWTRAVEILCSVLRGVQCAHEHGIVHRDLKPDNIF
                     VTETGTIKVLDFGIAKVMQSHDAGAGESPMPSPRELATGAPTDLTRAGTIMGTPQYMS
                     PEQWDTGIAIDHLTDLWACGILLYRMICGRHPLHPLDGERLIVTAAMEQPMPSMAEAA
                     PADVPPELVAIVDRCLRKDKAQRWQSAAELLAALERLVPGGRPLQRTR"
     gene            2720..>3075
                     /locus_tag="VF516_01730"
     CDS             2720..>3075
                     /locus_tag="VF516_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106410.1"
                     /translation="MAGTQTIDLDTHLAAIIAGETRAFGAWLAGAEPHVRDSLRSFAR
                     VIDVEAVLQETLLRVWQVAPRFIPDGRANGLLRLAIRIGRNLAISEVRRTRATPTGGD
                     DLEAALSAAEAPAAPDP"
BASE COUNT          433 a         1148 c         1086 g          408 t
ORIGIN      
        1 ttcacccagt cgatcggcaa cctgccggcg agcgcgacgc tgatcgcgcg gatcacgatc
       61 gacctgcggc tgacctggct acccgagggc gagtgggagc tgcggttccc caccgtgatc
      121 ggcccgcgct acgtcgcggc ggccgaccgc cccgaggacg tccgcggaac cgagatcgtc
      181 accgtgcccg acggcgtcgc ggcgcggatc cggatcgcga tcgcgatcga cgacacgctc
      241 ggcgccgggc gcaagccgac ctcgccgtcg cacgcgctca ccatcggcga cgacggcgtg
      301 gtcgagctcg ccgatcccgc cggcgcgcgg ctcgatcgcg acctcgtgct gcgctggccg
      361 gtcaccgggc cgaccaccgg attgtcgctc gcgatgatgc ggccggagca gggcgacgag
      421 tgcttcgggc tggtcacgat cgtcccgccc gcgccggcca cggtgacccg gacgttcgcc
      481 cgcgatctga tcgtgctgat cgacaccagc ggctcgatgg gcggccggcc gctcgacaag
      541 gccaagcagg tcatcgcgct cctgatcgag tcgctcggcg aggaggacgg gctcgagctg
      601 atcgcgttct cgtcccagcc gcggcgctac aagcccgccg ccgtccaggc cacccgcgac
      661 gagaagcagg cggcgatccg ctggctgcgc gcgctgtccg ccgggggcgg caccgagatg
      721 cggatcgcgg tgttcgaggc gctgcagcgg ctgcggcccg gcacgcagcg ccaggtcgtg
      781 atcgcgaccg acggctacat cgccgatgac acccggctgg tcagcgcgct gctcatccac
      841 ctgcccgccg gcggccggct gcacgtcgtg gggatcggct cgtcggtcaa tcgcgcgctg
      901 acccgggcgc tgtcgcgcgt cggccgcggc gcggaggtga tctgcgacct cgacggggat
      961 cccgagcgcg ccgtgaagcg gctgatcgac tgcacgcggt ggccggtcgc catcgacctc
     1021 gcgctcgagg gcagcgcgct ggtccagcgg gcgccctggg ccatccccga cgtgttctcg
     1081 gggtcaccgg tcgtcgtcgc ggtcaagctc caggccggcg acctggtggt gcgtggccag
     1141 gccatcgacg gcgagtgggt gtgccggctc cgggtctcgc tgccgcggcg gggggacgga
     1201 tctcccggga tcgccgcgct gtacggccgc gagcgggtcg ccgatcacga ggcccgctgc
     1261 ttcgccggcg agcgccgcga cgccgagatc gagcgcctcg ggatcacaca ccagatcacc
     1321 acccggtaca cggcgttcgt cgcgatcgac gaggcgctca cgacgcgccc gggcgcgcgc
     1381 gtcgaggtcg tgccacagga aatcccctac ggcaccagcg cagcggcgtt cggactgagt
     1441 agtccggacg cgagcctcgt ttccgaccgg atagcggtcg gtcgggcgct gctcaggggc
     1501 ctcgcacccg cagacatgat gttcggcagc gctgccggcg ccgctccgct ttctgccggg
     1561 ccgtccgtga ccggtggcag gatcctcgtc gagaacgaca tcgatgagat ccacgagacc
     1621 acgacccgga tcaccgacac ccggcgcccc ggggacacgt cgtccgaggt ccccaagctc
     1681 gagggcttca tggccgacat ccccggggag ccacccgcca catcggccga gccggtgcag
     1741 ggaccggggg acacgttcga tgccggcggg atcgacacct cgggaccgac cgtgaccgcc
     1801 ggcccggtgt tgccggtggg cggccggatc caccagtacg agacgatcag gatactcggc
     1861 cacggcggaa tgagcagcgt gttcctcgcc ctcgatctgc ggctcgtccg gctcgtcgcg
     1921 atcaagttcc tgccgtccag cgacccggag ctgacccagc ggttcctggt cgaggcacgg
     1981 gcgacggcgc gctgccagca cgagaacatc gtcagcatcc acgaggtcgg cgagcacgac
     2041 ggcgccccgt acatggtgct ggagtatctg cgcggcgagc cgctgcgccg gctgatcgag
     2101 aaccgccaga agctgccctg gacccgcgcg gtcgagatcc tgtgctcggt cctgcgcggc
     2161 gtgcagtgcg cccacgagca cggcatcgtc caccgcgacc tcaagcccga caacatcttc
     2221 gtcaccgaga ccggcacgat caaggtgctc gatttcggca tcgcgaaggt gatgcagtcg
     2281 cacgacgccg gcgctggcga gtcgccaatg ccgagcccgc gcgagctcgc gaccggcgca
     2341 cccaccgacc tgacccgggc cggcacgatc atgggcacgc cgcagtacat gtcgccggag
     2401 cagtgggaca ccggcatcgc gatcgatcac ctcaccgatc tctgggcgtg cggcatcctg
     2461 ctgtaccgca tgatctgcgg ccgccacccg ctgcatccgc tcgacggcga gcgcctgatc
     2521 gtcaccgcag cgatggagca gccgatgccg tcgatggccg aggccgcgcc ggccgacgtg
     2581 ccgcccgagc tggtcgcgat cgtcgaccgc tgcctgcgca aggacaaggc gcagcgctgg
     2641 cagtcggccg ccgagctgct ggcggcgctc gagcgcctcg tgcccggggg ccggcccctg
     2701 caacgtacca ggtagccccg tggccggcac ccagacgatc gacctcgaca cgcacctcgc
     2761 cgcgatcatc gcgggggaga cccgcgcgtt cggcgcgtgg ctcgccggcg ccgagccgca
     2821 cgtccgcgac agcctgcgct cgttcgcccg cgtgatcgac gtcgaggccg tgctgcagga
     2881 gaccctcttg cgcgtgtggc aggtcgcgcc gcggttcatc cccgacgggc gcgccaacgg
     2941 cctgctccgg ctcgcgatcc ggatcggccg caatctcgcg atcagcgagg tccgccgcac
     3001 ccgggccacg ccgaccggcg gcgacgacct cgaggccgcg ctctccgccg ccgaggcgcc
     3061 cgccgcgccc gatcc
//
LOCUS       DASVOR010000079         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_30073,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000079 DASVOR010000000
VERSION     DASVOR010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30073"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VF516_01735"
     CDS             complement(<1..544)
                     /locus_tag="VF516_01735"
                     /inference="COORDINATES: protein motif:HMM:NF012915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106411.1"
                     /translation="MEFRIAKAEFLRGLRLAQGIADRKSTMPMLANVLLRTQGKNQLL
                     VAATDLNVSLTAELKSHNASDGGIAIIAKSLFDLVANAPGDEVTLKKADNHWAEIKSG
                     KVTYRIVGMPDRDFPKVPDHREASYSTVESAVLREMIERTMFSICNDETRFHLNGVFF
                     ESDGSKCRMVSTDGHRLSKVE"
     gene            complement(825..>1574)
                     /locus_tag="VF516_01740"
     CDS             complement(825..>1574)
                     /locus_tag="VF516_01740"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEX8106412.1"
                     /translation="HEFRRNYRDGIDVLLVDDVQFLAGKESTQDEFFHTFNALHENHK
                     QIVLTADRKPHEISDIADRLRTRFAWGLLADIEPPELEVRIAILRKKAAVESVALPDD
                     VAMYIASSIKSNVRELEGALIRLAAYASLSKKRIDLEFAQETLGAAITRPREIITVES
                     VIKAVASYYGLKPSDIKSERRHKSVATPRAVAMYISRHHTKDSYPDLARAFGGKHHTT
                     VISAVQKIIERVKDDASLRSEIHAIESMILR"
BASE COUNT          246 a          480 c          542 g          306 t
ORIGIN      
        1 gctcgacctt gctcagccgg tggccgtcgg tcgacaccat ccggcacttc gagccgtcgc
       61 tctcgaagaa cacgccgttg aggtggaacc gggtctcgtc gttgcagatc gagaacatgg
      121 tgcgctcgat catctcgcgc agcaccgcgc tctcgaccgt cgagtagctc gcctcgcggt
      181 ggtcgggcac cttggggaag tcgcggtcgg gcatgccgac gatgcggtac gtgaccttgc
      241 cgctcttgat ctccgcccag tggttgtcgg ccttcttgag cgtcacctcg tcgccgggtg
      301 cgttggcgac cagatcgaac aggctcttgg cgatgatggc gatgccgccg tcgctcgcgt
      361 tgtggctctt gagctcggcg gtcagcgaga cgttgaggtc ggtcgccgcc accagcagct
      421 ggttcttgcc ctgggtgcgc agcaagacgt tggcgagcat cggcatcgtg ctcttgcgat
      481 cggcgatgcc ctgcgcgagg cgcagcccgc gaaggaactc ggcttttgcg atgcgaaact
      541 ccatggctcc tcgaccttcg tcgcttcctc cggatctgat caatcagaaa aagatctggt
      601 tggaaggatc agaactagat gctgtgggtt gtgtggatag atctccaacg ctccaggatg
      661 gctggtgaat atcaggaggg gctgacaggg aaacgtgcgc gctagccggt ggatgacacg
      721 aagcggccgt gtggacaacc ggggcgccgg cactcgtcca ccgcgggtcc acagcaaagt
      781 ccacaggata agtcacagcg gccggcatct tcgggtcccg ggattcaccg gaggatcatc
      841 gattcgatcg cgtggatctc ggagcggagc gacgcgtcgt ccttgacccg ctcgatgatc
      901 ttctggaccg cggagatcac cgtggtgtgg tgcttgccgc cgaacgcccg cgccaggtcg
      961 gggtacgagt ccttcgtgtg gtggcgcgag atgtacatcg cgaccgcgcg cggggtggcg
     1021 accgacttgt gccgccgctc ggacttgatg tcgctgggct tgaggccgta gtagctcgcg
     1081 accgccttga tcacgctctc gacggtgatg atctcgcgcg gccgcgtgat cgccgcgccc
     1141 agggtctcct gggcgaactc gaggtcgatg cgcttcttgg acagcgaggc gtacgcggcg
     1201 agccggatca gcgcaccctc gagctcgcgg acgttggact tgatcgacga cgcgatgtac
     1261 atggcgacgt cgtcgggcag cgccaccgac tcgaccgcgg ccttcttgcg caggatcgcg
     1321 atccggacct cgagctcggg cggctcgatg tcggcgagca ggccccaggc gaaccgggtg
     1381 cgcagccggt cggcgatgtc cgagatctcg tgcggcttgc ggtccgcggt caacacgatc
     1441 tgcttgtggt tctcgtgcag cgcgttgaac gtgtgaaaga actcgtcctg cgtgctctcc
     1501 ttgccggcga ggaactggac gtcgtcgacc agcagcacgt cgatgccgtc gcggtagttg
     1561 cggcggaact cgtg
//
LOCUS       DASVOR010000080         3529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_497684,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000080 DASVOR010000000
VERSION     DASVOR010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497684"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VF516_01745"
     CDS             <1..741
                     /locus_tag="VF516_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8106413.1"
                     /translation="MHLERGKLDDALRLRGGGRLAFAAIHPFDADHDRLDDVPADVRG
                     HRRYKDCGDFTAAGLAAAVAASGGGDIVVHSLANAPEVQRPLHRTSRAGYLAAVSTSA
                     YSHVALLHQLGPIASPGSSFLALSYLAAERVIPGYGGGMSSAKAALESDARVLAHEAG
                     RRWGHRVNIISAGPYPSRAAQAIGPIDRMIDHAARSSPLPAPITADEVAATAAFLSSP
                     LARGITGAVVHVDNGYSVMGIGADPPSP"
     gene            1116..1835
                     /locus_tag="VF516_01750"
     CDS             1116..1835
                     /locus_tag="VF516_01750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8106414.1"
                     /translation="MNNRNGTTHRNESNPWRLPTARLARIRKTAGEFGWPAALAAMAQ
                     GAVNRVAFFRVLKCIQVSKVDPEFLTIDPRFQHGFLDEATLRRLSGDPRYDLSPDFLD
                     EALAKRDECYGILDGDRLASFGWYSAVPTKIADNLRFCFDRRYVYMYKGFTDPEYRGQ
                     RLHALGMTSALQQFRERGAEGLVSYVYATNFDSLRSCYRMGYTDVGQIYCLHARDRYW
                     IHADASCRRAGVSVEALPDQR"
     gene            complement(1847..2899)
                     /locus_tag="VF516_01755"
     CDS             complement(1847..2899)
                     /locus_tag="VF516_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106415.1"
                     /translation="MRDFRLELALDDGRLLGVTHEGRLRMIDRDGHDAGGMRDRDRIV
                     PCGSVHSISVFRDRVLIKDKRELFVTDRALNLVERLASPPGGYAVFVGDGLVHHKDDR
                     IVFIDRAGRPRELCRVPVELAYDAMDRWEMETGTPALAGWITVNAAPGGDVTRSLVEA
                     AKDPSRGTALRRGGRLPGFAWRLGYLEPMTALFLVNSAVPPHLVICLSREGEPRWCTY
                     LSSGCCGGMPTALPDGRLAVSSGCGGIVSWLDRRGTVLARRKPFEGVGLASACGSVVR
                     PLADSGCVVDGGQGVTAYDASCELRWRSPTQCCQYDYDEQHGLLVTATWHDDEPRTWI
                     QIGCVKHLGSLHPASA"
     gene            complement(3182..>3529)
                     /locus_tag="VF516_01760"
     CDS             complement(3182..>3529)
                     /locus_tag="VF516_01760"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEX8106416.1"
                     /translation="PAEPSGSPSAVSLRSTPLPPERTSGAFDDDILGRGNGEMQPRVD
                     PYFKQIFDQFVSVKRRCNEPTAGLVYERFAEKLVKNREDLMLRTGCRDVRFTVYVKDG
                     KAALKATPVKDEV"
BASE COUNT          551 a         1289 c         1174 g          515 t
ORIGIN      
        1 atgcacctcg agcgcggcaa gctcgacgac gccttgcggc tgcgcggcgg cggccggctc
       61 gcgttcgccg cgatccaccc gttcgacgcc gaccacgacc ggctcgacga cgtcccggcc
      121 gacgtcaggg gccaccgccg ctacaaggac tgcggcgact tcaccgccgc cggcctcgcc
      181 gccgccgtcg ccgccagcgg cggcggcgac atcgtcgtgc acagcctcgc caacgccccc
      241 gaggtccagc ggccgctgca ccggacctcg cgcgccggct acctcgcagc cgtctccacc
      301 tcggcgtact cgcacgtcgc gctgctccac cagctcggcc cgatcgcgtc ccccggcagc
      361 tcgttcctcg cgctgtccta cctcgccgcc gagcgcgtga tccccggcta cggcggcggc
      421 atgtcctcgg ccaaggcggc gctcgagtcc gacgcccgcg tgctcgccca cgaggccggc
      481 cgccgctggg gccaccgcgt caacatcatc tcggccggtc cctacccctc gcgcgccgcc
      541 caggcgatcg gcccgatcga tcgcatgatc gaccacgccg cccgctcctc cccgctgccc
      601 gctccgatca ccgccgacga ggtcgcagcg accgcggcgt tcctgtcgag cccgctcgcg
      661 cgcggcatca ccggcgccgt cgttcacgtc gacaacggct actcggtgat gggcatcggc
      721 gccgatccgc cgtcgccctg acaccgccgg ctcacggccg ccggccagcg ccgtcgatcg
      781 cacgtgcgcg cacacccgac gccacacctg ccacgcgcga ccacgaggcc gcgtgctggg
      841 gcgaaatgcg acgagatgca agaatccgga tccacgcagg cgagcagtgg ggttaggcct
      901 cggtacaatc gtacgcaagc tgcacggagg tgtggcgaca gcgcgcgtgt ggcgttttgc
      961 gcttcgcgca atgcccttcg cgcgagcagc ttaccgctta ttaccccgag ctcgtcggcg
     1021 ggaacgacct ataccctcga gacagcgcgg agatggcgaa tggcgtttcc cctgcgtacg
     1081 acgcccaggg gaaaggactg gcaacggagg tcgccatgaa caatcggaat gggacaacgc
     1141 atcgcaacga atcgaatccg tggcggcttc ccaccgcccg gctggcccgc atccgcaaga
     1201 ccgccggcga gttcggctgg cctgcggcgc tggcggcaat ggcccagggc gccgtcaacc
     1261 gggtcgcgtt ctttcgcgtg ttgaagtgca tccaggtcag caaggtcgat cccgagttcc
     1321 tcacgatcga tccgcgcttc cagcacgggt ttctcgacga ggccaccctg cggcggctca
     1381 gcggcgatcc gagatacgat ctgtcgccgg actttctcga cgaggcgctg gccaagcgtg
     1441 acgagtgcta cggcatcctc gacggcgacc ggctggccag ctttggctgg tactcggccg
     1501 tgccgaccaa gatcgccgac aacctgcggt tctgcttcga ccggcgctac gtgtacatgt
     1561 acaagggctt caccgatccc gagtatcgcg gtcagcgcct gcacgcactg gggatgacct
     1621 cggcgctcca gcagtttcgc gagcgcggcg ccgagggcct ggtgtcgtac gtctacgcca
     1681 ccaacttcga ctcgctgcga tcctgctatc gcatgggcta taccgacgtg gggcagatct
     1741 attgtctgca cgcccgcgat cgctactgga tccacgccga cgcgagctgc cgcagggccg
     1801 gcgtctcggt cgaggcgctc cccgaccaga ggtgatccgg tcgtcgtcac gcgctcgccg
     1861 ggtgcaaaga gcccaggtgc ttgacacagc cgatctggat ccacgtcctg ggttcatcgt
     1921 cgtgccacgt cgcggtgacg agcagcccat gctgctcgtc gtagtcgtac tggcagcact
     1981 gggtgggcga tcgccagcgc agctcgcagc tggcgtcata ggcagtgacg ccttgtccgc
     2041 catcgaccac gcacccggag tccgcgaggg gtcgcacgac gctgccacag gcagacgcga
     2101 ggccgacgcc ttcaaagggc ttgcgccgcg cgaggactgt accgcggcgg tcgagccacg
     2161 acacgatccc gccgcagccc gagctgacgg cgagccggcc atcgggcagc gctgtcggca
     2221 tgcctccgca gcacccggag ctgagatacg tgcaccagcg cggctcgccc tcgcggctca
     2281 agcagatcac caggtgcggc ggaactgcgc tgttgaccag gaacagcgcc gtcatgggct
     2341 cgaggtagcc caggcgccag gcgaagccgg gcaggcgacc cccgcgccgc agcgccgtgc
     2401 ccctggacgg atccttcgct gcttcgacca gcgagcgggt gacgtcgccg cccggtgcgg
     2461 cattcacggt gatccagccg gcgagggccg gggttccggt ctccatctcc cagcgatcca
     2521 tcgcgtcgta cgccagctcg acgggaacgc ggcagagctc ccgggggcgc ccggcccggt
     2581 cgatgaagac gatgcgatcg tccttgtggt ggaccagacc gtcaccgacg aacacggcgt
     2641 atccgccggg aggcgacgcg agacgctcga cgaggttcag cgctcgatcg gtcacgaaca
     2701 gctcgcgctt gtccttgatc agcacgcgat cgcgaaacac cgagatcgag tggacgcttc
     2761 cgcacgggac gatgcgatcg cggtcgcgca tccctccggc gtcatggccg tcgcgatcga
     2821 tcatgcggag ccgaccctcg tgcgtgacac ccaggagtcg gccatcgtcg agcgcgagct
     2881 cgagccggaa gtcgcgcacg gaccagaggg tttgcccctg gccatcgagg cagaccaccg
     2941 catgaggacc aggagacccg cggccgcgct ggttgtcgac gtagctgcag acgatacgtt
     3001 cgcccgcgaa cgcgatgccg gacggcgagc gacccggcat cgtgcaagac cagcagacct
     3061 caccggtggg aaccatgacg cccgccgacc gcagcgcgag ctgcgacgct tgcggcgggc
     3121 cgcccgccct cgagcggccc acgccggcaa ggttcatcca ccggcgtcgc gcgctcgacg
     3181 gtcagacctc gtccttgacc ggcgtcgcct tgagcgcggc cttgccgtcc ttgacgtaga
     3241 cggtgaaccg gacgtcgcgg cagccggtcc tgagcatcag gtcctcgcgg ttcttgacca
     3301 gcttctcggc gaaccgctcg tagaccaggc ccgcggtcgg ctcgttgcag cggcgcttga
     3361 ccgagacgaa ctggtcgaag atctgcttga agtaggggtc gacgcgcggc tgcatctccc
     3421 cgttgccgcg accgaggatg tcgtcgtcga aggcgccgga ggtccgctcg ggcggcagcg
     3481 gggtcgagcg aagcgagacg gcggatgggg accccgacgg ctctgccgg
//
LOCUS       DASVOR010000081         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_351558,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000081 DASVOR010000000
VERSION     DASVOR010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351558"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="VF516_01765"
     CDS             <1..391
                     /locus_tag="VF516_01765"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEX8106417.1"
                     /translation="ISLPGGGYQASDGDLRVTAIRETREELGVELDGAHLLGHLDPLH
                     PRTSGPSGIEVTPFVFATPVTLEPVCGPEALAAFWLPLDLAASGALDGTYSYPASSVT
                     FPCWTYEGHVIWGLTRRILDLVLATAV"
     gene            complement(399..1559)
                     /locus_tag="VF516_01770"
     CDS             complement(399..1559)
                     /locus_tag="VF516_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8106418.1"
                     /translation="MMVRVEHTSKVHLGRVLVVDDDPAMRRLCARTLGHEGWDVVVVD
                     GGKAALDCVAARGVPFDCVVSDVHMPGMDGFALIAAIRHHDDDLPVLLMTGDPSLDGA
                     VRAIDTGAVSYLSKPFDHETLAAAVARAARRHGVARMRRRVTSEHDAHDTAQRFDAAL
                     ARSWMAFQPIFDVAAHRTFAYEALVRTDEESLKRADVLIATAERLGRIHDLGRTVRTA
                     VAQMARQVPDGMLIFVNVHGLELTDEDLFSDSCALAPLAPRVVLEITERIGLDAVAGP
                     ARVEMLRRRGYRIAVDDLGAGYAALGALATLEPEVVKLDMSLIRDIDAHATKRRVVGA
                     IATLCRELGSRVVAEGVETPSELAAVREVGIELIQGYLLARPTRDIAVVPLL"
     gene            1718..>2264
                     /locus_tag="VF516_01775"
     CDS             1718..>2264
                     /locus_tag="VF516_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106419.1"
                     /translation="MRSVWPSIEIVAPRPRGMRSPRSTDDLGERLGVADLSAWSVIAV
                     MCTAVDDREAGRPRAVVRGASSWYYPRASVTAVSGPHLEASRLAEHVDETGAPCTRHP
                     AISPASLYQLAMVGSRAPAFHHDCASKLQGLVMALDELSELTENGDPQLIRAVETALE
                     ASRELNALLNLNRTLTRPATRT"
BASE COUNT          313 a          847 c          797 g          307 t
ORIGIN      
        1 catctcgctc ccgggcggcg gctaccaggc gagcgatggc gacctccggg tgaccgcgat
       61 tcgcgagacc cgcgaggagc tcggcgtcga gctcgacggc gcgcacctgc tcggccacct
      121 cgatccgctg cacccgcgga cgtcgggccc cagcgggatc gaggtcacgc cgttcgtgtt
      181 cgcgacgccg gtcacgctcg agccggtgtg cggcccggag gcgctcgccg cgttctggct
      241 gccgctcgac ctcgctgcgt cgggcgcgct cgacggcacc tacagctacc ccgcgtcgtc
      301 ggtgacgttc ccgtgctgga cctacgaagg ccacgtgatc tgggggctga cccgccggat
      361 cctcgacctc gtgctggcga ccgcggtcta ggcgcggctc acagcagcgg gacgacggcg
      421 atgtcgcggg tcggccgggc gagcaggtag ccctggatca gctcgatgcc gacctcgcgc
      481 accgcggcga gctcggacgg ggtctcgacg ccctcggcga cgacccggct gccgagctcg
      541 cggcacagcg tcgcgatcgc gccgaccacg cggcgcttgg tggcgtgggc gtcgatgtcg
      601 cggatcagcg acatgtcgag cttgacgacc tcgggctcga gcgtggccag cgcgcccagc
      661 gcggcgtagc ccgcgccgag gtcgtcgacc gcgatccggt agccgcgccg gcgcagcatc
      721 tcgacccgcg ccggtccggc gaccgcgtcg agcccgatcc gctcggtgat ctcgaggacg
      781 acccgcgggg cgagcggcgc cagcgcgcag ctgtcggaga acagatcctc gtcggtcagc
      841 tcgaggccgt gcacgttgac gaagatcaac atgccgtcgg gcacctgcct ggccatctgc
      901 gccaccgcgg tgcgcacggt gcggccgagg tcgtggatcc ggccgagccg ctcggcggtc
      961 gcgatcagca cgtcggcgcg cttgagcgac tcctcgtcgg tgcggaccag cgcctcgtag
     1021 gcgaacgtgc ggtgggcggc gacatcgaag atcggctgga acgccatcca tgaccgcgcg
     1081 agggcggcgt cgaaccgctg ggcggtgtcg tgggcgtcgt gctcggacgt cacgcgccgc
     1141 cgcattcgcg cgacgccgtg gcgccgggcc gcgcgcgcga ccgccgccgc gagggtctcg
     1201 tgatcgaacg gcttggacag gtacgacacc gcaccggtgt cgatcgcgcg gaccgcgccg
     1261 tcgagcgacg gatcgccggt catcagcagc accggcaggt cgtcgtcgtg gtggcggatc
     1321 gcggcgatca gcgcgaagcc gtccatcccc ggcatgtgga cgtcggacac cacgcagtcg
     1381 aacggcacgc cgcgcgccgc gacgcagtcc agcgccgcct tgccgccgtc gaccaccacg
     1441 acgtcccagc cctcgtggcc gagcgtccgg gcgcacagcc gccgcatcgc cggatcatca
     1501 tccacaacca ggacccgccc gaggtgaacc ttcgaggtat gctcgacccg caccatcatc
     1561 tacctgcgat cgaccttacc ccaaccctcg ccgcccggcc gcgctgcgaa acgcgtcacg
     1621 cccgaccggg caccgcccgc cagcggacaa atacaggccc atcccacgct tgccgccttt
     1681 ggcggcggcg ttccaacgcc gcgcgtcgaa ctgtgccatg aggtcggtct ggccgtcgat
     1741 cgagatcgtg gcgccacggc ccagggggat gcgctcaccc cggagcaccg acgacctcgg
     1801 cgaacgcctc ggcgtggctg acctctcggc gtggtcggtc atcgcggtga tgtgcacggc
     1861 ggtcgacgat cgcgaggccg ggcggccgcg cgctgtcgtg cgcggtgcat cgagctggta
     1921 ctatccgcga gcgagcgtga cggcggtgtc cggcccccat ctcgaagctt cgcggctcgc
     1981 cgagcacgtc gacgagaccg gcgcgccgtg tacgcgccat ccggcgatct cgccggccag
     2041 cctgtaccag ctcgcgatgg tcggctcgcg tgcgcccgcg ttccaccacg actgcgcgtc
     2101 caagctgcag ggcctggtga tggcgctcga cgagctctcc gagctgaccg agaacggcga
     2161 tccgcagctg atccgcgcgg tcgagaccgc gctcgaggcg tcgcgcgagc tcaacgcctt
     2221 gctcaacctc aaccgcacgc tgacccgccc ggcgacccgg acct
//
LOCUS       DASVOR010000082         1770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_30096,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000082 DASVOR010000000
VERSION     DASVOR010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30096"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1241)
                     /locus_tag="VF516_01780"
     CDS             complement(213..1241)
                     /locus_tag="VF516_01780"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX8106420.1"
                     /translation="MQHAAIDLGSRESQICIRQPDGAIVEERKLPTRKLPEVFKTWPT
                     SRVVMEASAEAFKIADAALAAGHQVRVVPGKLVRLLGVGDRGVKNDQRDARQLSQASW
                     QTDVPSVHIPSAAARELKSICGARDVLVATQRNLSNNVRGWLRTQLWKLPGGTMVTLP
                     ERLRTHARASGRELPEHIERQLTMLTQVTEQVQAANKQARKLAMEHPVCRRLMTVPGV
                     GPLTSLRFVAAIDDPSRFATAHRVQSYVGLTPGEHSSCGVGGKRGITKAGPSELRRCL
                     VQAAWSVMLARRSHPMLEWVRQIAQRRNRPVAVIALARKLAGILFAIWRDGTTYEANH
                     AAGRSALT"
     gene            1440..1649
                     /locus_tag="VF516_01785"
     CDS             1440..1649
                     /locus_tag="VF516_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106421.1"
                     /translation="MTGEVGRHRERRRGRLLRQVDQVSHMFTALAITDSLEQIVGGME
                     QGIGTHELIAEVFHLADDIERGSRW"
BASE COUNT          254 a          612 c          595 g          309 t
ORIGIN      
        1 ggagtggctg gcgtccgggt tggaggtgtc tggctgtagc agtcgactat tcgcgctctg
       61 gtggcgcagt agggggtccg tcggcggcgg cgcactcgag gtaacgcggt tctcatcgga
      121 agcccgacga gccggaatcg cgcgtgtcgc gctcaccgcc tctggacagc catcacctgg
      181 gtccgaggca catgacgtgg gtcgggcgag tgctacgtca gagcgctcct ccccgctgcg
      241 tggttggctt cgtaggtcgt cccgtcgcgc cagatcgcga acaggatgcc ggcgagtttt
      301 cgggccagcg cgatgactgc gacgggtcgg ttgcgccgct gagcgatctg gcggacccac
      361 tcgagcatcg gatgcgacct gcgagccagc atcaccgacc atgccgcttg taccaggcag
      421 cgtctgagct cgctcgggcc cgccttggtg atccctctct tgcccccaac tccacagctc
      481 gagtgctccc cgggcgtgag ccccacgtag gactgcaccc ggtgcgcggt cgcgaaccgg
      541 ctcgggtcgt cgatcgctgc cacgaagcgc agcgacgtga ggggtccgac gccgggaacg
      601 gtcatcagcc ggcgacacac ggggtgctcc atcgcgagct tgcgagcctg cttgttcgct
      661 gcctgcactt gctcggtcac ctgggtcagc atcgtgagct gccgctcgat gtgctcgggc
      721 agctcgcgcc cgctggcgcg cgcgtgggtc cgcagtcgct ccggcagggt caccatcgtc
      781 ccgccgggaa gcttccacag ctgcgtccgc agccagcccc ggacgttgtt ggacaggttg
      841 cgctgcgtcg cgaccagcac gtcgcgagcg ccgcagatcg acttgagctc gcgtgccgct
      901 gccgacggga tgtggaccga cggcacgtcc gtctgccacg acgcctggct cagctggcga
      961 gcatcccgct ggtcgttctt gactccccga tcgcccacgc cgagcaagcg caccagcttc
     1021 cccggcacca cccgcacctg gtgccccgcg gcgagggctg cgtcggcgat cttgaacgcc
     1081 tccgctgagg cctccatcac gacccgactc gtcggccagg tcttgaagac ctccggcaac
     1141 ttccgagtcg ggagcttgcg ctcctcgacg atggctccgt cgggctgacg gatgcagatc
     1201 tgcgattccc tgctccccag atcgatggca gcatgctgca tggctgtcct ccaaggtgtg
     1261 cgcttcgagc gcgtttctag ctgaggcacg gtctaccgcg acctcgttgc cgagggggac
     1321 agccgctttt gctcgtcgag caggggcagc cgacggctgc cgcactggct gtccgtcgag
     1381 tgcaggaagg cacgtcgtgc gtcggtccgg cgtcatcctt ccgacctccc cttgacaagt
     1441 tgacgggcga ggtcggccgc catcgcgagc gccgtcgcgg tcgcctgctt cgccaggtcg
     1501 accaggtgag ccacatgttc acggcgctgg cgattaccga ctcgctcgag cagatcgttg
     1561 gcggcatgga gcaaggcatc ggcacgcacg agctgatagc ggaggtgttc cacctcgcgg
     1621 atgacatcga acgaggttcc aggtggtagg cggcgcatga ctaccacccc ccttgcgagg
     1681 gctccggact cgccgcgtca tcggcccacg ggtcgcgagc cgcatcgtcg agcacgaaat
     1741 ggaactccgc gccgccctcg gagacctcgc
//
LOCUS       DASVOR010000083         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_322361,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000083 DASVOR010000000
VERSION     DASVOR010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322361"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VF516_01790"
     CDS             complement(<1..226)
                     /locus_tag="VF516_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106422.1"
                     /translation="MSHTLARLRDTVGDPLFVRAGNRLVPTPRAVAMRERVARVANEI
                     GDLLRPERPLDVATLERTFVVRASDATIMIL"
     gene            396..>1723
                     /locus_tag="VF516_01795"
     CDS             396..>1723
                     /locus_tag="VF516_01795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8106423.1"
                     /translation="MTLSRRRQLAILATCCLSIVLVTMDVTIVNVALPAIRSDLHASV
                     AGLQWSIDGYTVIVASFLLLAGSMADRFGRRKTFQLGLCVFSVGSLLCSLAPSTPALV
                     AFRMLQAVGGSMLNPVAMSIVVNTFLDPRERARAIGIWGAVFGVAMAAGPPLGGLLIE
                     TIGWRSIFWVNVPFGILALVLTARVVPESRAERPRRLDLVAQAFVIVALLALTSAVID
                     GRRAGWTSWSIAAGFSTALACIAGLVVWESRRREPMLDLRLFRSIPFASATLMAVVAF
                     AAFNGFLFLNSLYLQESRGMRASTAGLATLPVALTLVVCSPLSGRLVGAGRARVALVV
                     AGAAMAIGALLLTALAPGTSIAYLVVAYAIFGVGLGIVNTPITNTAVSGMPRSQAALA
                     SAVTSTSRQVGASLGVALAGSLAKAGIESAHRPDFADATHIVFWMIAGFGA"
BASE COUNT          193 a          617 c          608 g          305 t
ORIGIN      
        1 ccaggatcat gatggtggcg tcggacgcgc gaaccacgaa cgtccgctcg agcgtcgcga
       61 cgtcgagcgg acgctcgggc cggagcagat caccgatctc gttcgcgacg cgcgcgacgc
      121 gctcccgcat cgcgaccgct cgcggcgtcg gcaccaggcg gttgccggcg cgcacgaaca
      181 gcggatcgcc gaccgtgtca cgcaggcggg cgagcgtgtg gctcatcgcg ggcgggcctg
      241 acgttcatgc ggcgcgcggc cgccgtgacg ctgccttccc gcagcagcgc gtcgagggcg
      301 agcaggaggt tggcatccgc ggattgcatc tggtgcatgt accacgttcg caggtttcaa
      361 tcgattcatc ccgccgggcg ccctaggttc accgcatgac cctgtcccgt cgccgccagc
      421 tcgccatcct cgcgacgtgc tgcctctcga tcgtgctggt cacgatggac gtgaccatcg
      481 tcaacgtcgc gctccctgcg atccgcagcg acctgcacgc cagcgtcgcg gggctgcagt
      541 ggtcgatcga cggctacacg gtcatcgtcg cgagcttcct gctcctcgcc ggctcgatgg
      601 ccgaccgctt cggccgccgc aagaccttcc agctcggcct gtgcgtgttc agcgtgggct
      661 cgctgctgtg cagcctcgcc ccgagcacgc ccgcgctcgt ggcgttccgc atgctgcagg
      721 cggtcggcgg atcgatgctc aaccccgtcg ccatgtcgat cgtcgtcaac acgtttctcg
      781 acccccggga gcgcgcacgc gccatcggca tctggggcgc ggtgttcggc gtggcgatgg
      841 cggcggggcc tccgctcggc ggcctgctga tcgagaccat cggctggcgg tcgatcttct
      901 gggtgaacgt gccgttcggg atcctggccc tcgtgctgac cgcgcgcgtc gtccccgagt
      961 cgcgcgcgga gcggccccgg cgcttggatc tcgtcgcgca ggcgttcgtg atcgtggcgc
     1021 tgctcgcact gacctcggcc gtgatcgatg gccggcgcgc gggctggacg tcatggtcga
     1081 tcgcggcggg cttctcgacg gcgctggcgt gcatcgcagg gctcgtggtg tgggagtctc
     1141 gccgccgcga gccgatgctc gacctgcggc tgtttcgcag cattccgttc gcgtcggcca
     1201 cgctcatggc ggtggtcgcg ttcgccgcgt tcaacggctt tctgttcctg aactcgctct
     1261 accttcagga atcgcgcggg atgcgcgcgt cgacggcggg cctcgcgacc ctcccggtcg
     1321 ccctgacgct cgtggtgtgc tccccgctct ccgggcgcct cgtcggtgcg ggccgtgcac
     1381 gggtcgcgct cgtcgtggcg ggggccgcga tggcgatcgg cgcgctgctg ctgacggcgc
     1441 tcgcgccggg gacgtcgatc gcgtacctgg tcgtcgcgta cgcgatcttc ggcgtcggcc
     1501 tggggatcgt caacacgccg atcaccaaca cggcggtgtc cgggatgccc cgatcccagg
     1561 ccgcgctcgc atcggccgtc acctcgacca gccggcaggt gggagcgtcg ctcggggtcg
     1621 cgctcgccgg ctcgctcgcg aaggccggga tcgagtccgc gcaccggccg gacttcgccg
     1681 acgccaccca catcgtgttc tggatgatcg ctggattcgg cgc
//
LOCUS       DASVOR010000084         5294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410056,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000084 DASVOR010000000
VERSION     DASVOR010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410056"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF516_01800"
     CDS             <1..275
                     /locus_tag="VF516_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106424.1"
                     /translation="QQRRVIAAGQIARELFTIAVVLGRASGAGVGHRDQQLAAVYCAG
                     AMARLPALWPELTDDTAARDAEIEAVLEADPVVDLHHDDVLAGRAG"
     gene            complement(213..2846)
                     /locus_tag="VF516_01805"
     CDS             complement(213..2846)
                     /locus_tag="VF516_01805"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8106425.1"
                     /translation="MGLALLAALGSVARADGAPPVVRAHAVRRTGPITVDGQLDEPSW
                     AAAPKQAGFLQRFPRDAASPSAETRFAILYDDDAIYVGVWADDPRPDLIRALLTRRDA
                     DAPADGVTVAFDSYHDRRTAYAFQLNAAGVQRDMLLFDDSGLDDTWDAVWTGNAAITP
                     TGWTAEYRIPLGQLRFAEHDAQEWGLQLVRLIGRTGEQDSWSPWPRSAPQVVSKFGIL
                     DGIDHLVAARRLELLPYTTGGIDRTPVAAGDPLHQKYSWRRNAGLDLKYGLGPAFTVS
                     ATINPDFGQVEADPSKVNLTASELFFAEKRPFFLEAVDLFKLPLGDPDASVESAFYSR
                     RIGATPADPGTDYTFIKAPQSTTIYGAAKLTGKTADGWSVGVLDAITGPETAETFTAP
                     PSTTPDAPGMRAEPAVAALTNYAIARIKRDLGEGKTAVGLSATAVDRKLDDPALAARV
                     HDQAYTGGLQLQHRWADNVWTANVSLIGSWVHGTEAAIAATQQSSVHYLQRPDLEGGS
                     RFDPTRTSLSGLGASWKIGALGDTRHWRFGLFGDLRTAGLELNDAGFQINSNRTVPFF
                     RAQYHDDEPGGHVLNWALTSDFWAQYTLEPRLTDYGVECDGNVQFTNYWSLAAGCNLV
                     RAKWNVDLLRGGPALRLGPRMFSYLNLATDTRERVWFSLAGTAGHNGTTGELDVNVTL
                     GATIQALPNLDLSIGPSLYVRNDPLQYVADSVDLAGQHHIVLGRIRETDVSTTLRVNW
                     AFSPHLALQAYAQPYIAAGRYSELKDVDHPAAVRYADRFHVLAGDEIQRVGGSYQVAR
                     DGAMWSFDRPDFNFLQLRSTVVMRWEYRPGSTVFAIWSHGRTSDTFQDGRFQLGRDVS
                     NLAGTASENIVMVKVNYWVGL"
     gene            complement(3022..3570)
                     /locus_tag="VF516_01810"
     CDS             complement(3022..3570)
                     /locus_tag="VF516_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106426.1"
                     /translation="MNLGRGTGMEGAAVLLVDDDELWVRACERGFRSMGCAVRAATSV
                     AGALAVASEQAPDLVVADVRLGAECGIDLIEPLRRAHPDCTIALVSAYLSVALTAVAV
                     RKGAHLVLFKPIHPTEIVRRLPQLDVDDPPCRTPTLARAEWEHIQRVLLDCNGNVSEA
                     ARRLGILRQSLQRRLRKHAPPE"
     gene            complement(3674..>5294)
                     /locus_tag="VF516_01815"
     CDS             complement(3674..>5294)
                     /locus_tag="VF516_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106427.1"
                     /translation="PAPGAARRFAAAAAAGVPLPIPTSTQFDITGFLQEAHTAGPDAP
                     GTLRVNGQVVTIPVNTVVILPAAGLTWDELFTQAPAPYGPAQTGLAMFDTPAPLTTSE
                     VHVIGNRVTTAAGGDQYIAGLVYLSQQSLNAGAGFINFIDYGAGELRVGGLLGDPSTG
                     ARVRIIEGQQSQIPDTPGAQTAANGLVFGQYHAPILEYIFPENVPGTPIVPNNFEAIP
                     FLACGGMTFSDGTVAGPLQPWPGAVAPVCANAPSAPVANAGAAVTVASGATVTLAGSA
                     TGTAPLAFSWAQTAGPTVALSDPTIASPSFAAPAVTVTTALTFALTVTNAVGSSTATV
                     TVTVNAAAAPTVVHIADRTVASGTPVSMTATCSDPNGGTCSFVWTQTSGTPVVLTPNP
                     FAGATVSFQVALAAGAAPAVLQFQIVATSSAGVSSAPDLTAVTVTSPPDTVQITSAEY
                     RADKQRLILTATSSVVSPNVNLTLQPYVTATGTTFDPAAIGNTFTNGGAGTYTLTVVG
                     APQPAAPPATPLTARSSAGGVSPPHGLDRLR"
BASE COUNT          716 a         1843 c         1975 g          760 t
ORIGIN      
        1 agcagcagcg ccgggtgatc gccgccgggc agatcgcccg cgagctgttc acgatcgcgg
       61 tcgtgctcgg ccgggccagc ggcgccgggg tcggtcaccg cgatcagcag ctcgccgcgg
      121 tgtactgcgc cggcgcgatg gcgcggctgc ccgcgctgtg gcccgagctg accgacgaca
      181 ccgccgcgcg ggacgccgag atcgaggcgg tgctagaggc cgacccagta gttgaccttc
      241 accatgacga tgttctcgct ggccgtgccg gctaggttgg acacgtcgcg gccgagctgg
      301 aaccgaccgt cctggaacgt gtcgctggtc cgtccgtggc tccagatcgc gaacacggtg
      361 gagcccggac ggtactccca ccgcatcacc acggtggagc gcagctggag gaagttgaag
      421 tcggggcgat cgaagctcca catcgcgccg tcgcgcgcga cctggtagct gccgccgacc
      481 cgctggatct cgtcgcccgc gagcacgtga aaccggtcgg cgtaccggac ggccgcgggg
      541 tggtcgacgt ccttgagctc gctgtagcgg cccgccgcga tgtacggctg ggcgtaggcc
      601 tgcagcgcca gatgcggcga gaacgcccag ttcacgcgca gcgtggtcga cacgtcggtc
      661 tcgcggatcc gcccgagcac gatgtggtgc tggccggcga gatcgacgga atcggcgacg
      721 tactgcagtg gatcgttgcg cacgtagagg ctcggcccga tcgacaggtc gaggttgggc
      781 agcgcctgga tcgtcgcgcc cagcgtgacg ttgacgtcga gctcgccggt cgtcccgttg
      841 tggccggcgg tcccggccag gctgaaccac acgcgctcgc gcgtgtcggt cgccaggttg
      901 aggtagctga acatgcgagg accgagccgc agggccgggc cgccgcgcag caggtcgacg
      961 ttccacttcg cgcgcacgag gttgcagccc gcagccagcg accagtagtt cgtgaactgc
     1021 acgttgccgt cgcactcgac gccgtagtcg gtcagccgcg gctcgagcgt gtactgggcc
     1081 cagaagtcgg aggtgagcgc ccagttgagc acgtgacccc ccggctcgtc gtcgtgatac
     1141 tgcgcccgga agaacgggac cgtccggttc gagttgatct ggaacccggc gtcgttgagc
     1201 tcgaggccgg cggtccgcag gtcgccgaac aggccgaacc gccagtgccg ggtatcgccc
     1261 agcgcgccga tcttccacga cgcgcccaga cccgacaggc tggtgcgcgt cggatcgaac
     1321 cggctgccgc cctcgaggtc cggacgctgg agatagtgga ccgagctctg ctgggtcgcg
     1381 gcgatcgccg cctcggtgcc gtgcacccag ctcccgatca ggctgacgtt cgcggtccac
     1441 acgttgtccg cccagcggtg ctgcagctgc aggccgccgg tgtacgcctg atcgtggacc
     1501 cgcgccgcga gcgccggatc gtcgagcttg cgatcgaccg cggtcgcgga caggccgacc
     1561 gcggtcttgc cctcgccgag atcgcgcttg atgcgcgcga tcgcgtagtt ggtcagcgcc
     1621 gcgaccgccg gctcggcgcg catgcccggc gcatccgggg tcgtcgacgg aggcgcggtg
     1681 aaggtctcgg cggtctcggg gccggtgatc gcgtcgagca cgcccaccga ccagccgtcg
     1741 gccgtcttgc ccgtcagctt ggcggcgccg tagatcgtcg tcgactgcgg cgccttgatg
     1801 aacgtgtagt cggtaccggg atcggccggg gtcgcgccga tccggcggct gtagaacgcg
     1861 ctctcgacgc tggcgtcggg atcgccgagc ggcagcttga acaggtcgac ggcctcgagg
     1921 aagaacggcc gcttctcggc gaagaacagc tcgctggccg tcaggttgac cttcgaggga
     1981 tcggcctcga cctggccgaa gtcggggttg atcgtcgccg acaccgtgaa cgcaggcccc
     2041 aggccgtact tcaggtcgag gccggcgttg cggcgccagc tgtacttctg gtggagcgga
     2101 tcgccggccg cgacgggcgt gcggtcgatc ccgccggtgg tgtacggcaa gagctcgagc
     2161 cggcgcgccg cgaccaggtg atcgatgccg tcgaggatcc cgaacttgct gaccacctgg
     2221 ggcgcgctgc gcggccacgg cgaccacgag tcctgctcgc cggtccgccc gatcaggcgt
     2281 acgagctgga ggccccactc ctgcgcgtcg tgctcggcga accgcagctg gccgagcggg
     2341 atccggtact ccgcggtcca gccggtcggt gtgatcgccg cgttgccggt ccacaccgcg
     2401 tcccaggtgt cgtccaggcc ggagtcgtcg aacagcagca tgtcgcgctg cacgccggcc
     2461 gcgttgagct ggaacgcata ggcggtccgg cgatcgtggt agctgtcgaa cgccaccgtc
     2521 accccgtcgg cgggcgcatc ggcgtcgcgg cgggtgagca gcgcgcggat cagatcgggc
     2581 cgcggatcgt cggcccacac cccgacgtag atcgcgtcgt cgtcgtagag gatcgcaaac
     2641 cgggtctcgg ccgacgggct ggccgcgtcc ctggggaacc gctggaggaa gccggcctgc
     2701 ttgggcgcgg ccgcccagct cggctcgtcg agctggccgt cgaccgtgat cgggccggtc
     2761 cggcgcaccg cgtgcgcgcg cacgaccggc ggagcgccgt ccgcgcgggc gaccgagccc
     2821 agggcggcga gcagtgcgag acccacgcgc agcatgtcga cgacgccgcc gagggtactc
     2881 gatcaccctc cgcgacgcgc gcaccgggat gccgatctca tccgccgcac gacgcgcatc
     2941 tgcgacgagc gtcacatcgc cacgtcgcgc gcgaagcggc gccgccctcg tcgcccggta
     3001 cgctcgttcg tgcggcgcgg atcactccgg cggagcgtgc ttgcgcagcc ggcgctgcag
     3061 cgactggcgc aggatgccga gccggcgcgc ggcctcgctg acgttgccgt tgcagtcgag
     3121 cagcacgcgc tggatgtgct cccactcggc gcgcgccagc gtcggcgttc tgcacggggg
     3181 gtcgtcgacg tcgagctgcg gcagccggcg cacgatctcg gtcgggtgga tcggcttgaa
     3241 cagcaccagg tgcgcgccct tgcgcaccgc gaccgcggtc agcgcgaccg acaggtaggc
     3301 gctgaccagc gcgatcgtgc agtcggggtg ggctcggcgc agcggctcga tcaggtcgat
     3361 gccgcactcg gcgccgagcc ggacgtccgc gaccacgaga tccggcgcct gctcgctcgc
     3421 gaccgccagc gcgccggcca cgctggtcgc cgcgcgcacc gcgcagccca tgctgcggaa
     3481 gccgcgctcg cacgcccgga cccacagctc atcgtcgtcc accagcagca ccgccgcacc
     3541 ctccatgccg gtccccctgc ccaagttcat cacgtcgtgg tggaacgtcc aacccgcacg
     3601 atgtgaaggc gcgcgtcgcg gagatgtgac gcccggaaat gttcggcaag ccggcccgac
     3661 ggggtgccgc agatcagcgc aggcgatcga gtccgtgggg cgggctgacc ccgccggcgc
     3721 tcgatcgggc cgtcagcggc gtcgccgggg gcgccgccgg ctgtggtgcg ccgaccacgg
     3781 tgagcgtgta cgtcccggca cccccgttgg tgaaggtgtt gccgattgct gcggggtcga
     3841 acgtcgtccc ggtggcggtc acgtagggct gcagcgtcag gttgacgttc gggctgacga
     3901 ccgacgacgt ggcggtcagg atcagccgct gcttgtcggc acggtactcg gcgctggtga
     3961 tctggaccgt gtcgggcggc gaggtcaccg tgaccgcggt gaggtcgggc gccgagctga
     4021 cgcctgcgct gctggtcgcg acgatctgga actgcaagac cgccggcgcc gcgcccgcgg
     4081 ccagcgcgac ctggaagctg accgtggcgc ccgcgaacgg gttcggcgtc agcacgaccg
     4141 gcgtgccgga ggtctgcgtc cacacgaagc tgcaggtccc tccgttcgga tcgctgcacg
     4201 tggccgtcat cgacaccggg gttccggagg cgacggtccg gtcggcgatg tgaacgacgg
     4261 tgggcgccgc cgcggcgttg accgtgacgg tcacggtcgc ggtcgacgat cccaccgcgt
     4321 tggtcaccgt gagcgcgaag gtcagcgcgg tcgtcacggt gaccgcgggt gcggcgaagc
     4381 tcgggctggc gatcgtcggg tcggacagcg cgacggtggg tcccgcggtc tgcgcccacg
     4441 agaacgccag cggcgcggtg ccggtggcgc tgcccgccag cgtcacggtc gcccccgagg
     4501 ccacggtcac ggcggcaccg gcgttcgcga ccggcgcgct cggcgcgttc gcgcacaccg
     4561 gcgccaccgc gccgggccac ggctggagcg gaccggcgac cgtgccgtcg ctgaacgtca
     4621 tgccgccgca ggccaggaac ggaatggcct cgaagttgtt gggcacgatc ggcgtgcccg
     4681 ggacgttctc ggggaagatg tactcgagga tcggcgcgtg gtactggccg aagaccagcc
     4741 cgttggcggc ggtctgcgcg ccgggcgtgt ccgggatctg gctctgctgg ccctcgatga
     4801 tccggacccg cgcgccggtg ctcggatcgc ccagcagacc gccgacccgc agctcgccgg
     4861 cgccgtagtc gatgaagttg atgaacccgg cgccggcgtt gagcgactgt tgcgacaggt
     4921 agaccaggcc ggcgatgtac tgatcgccgc cggcggcggt ggtcacccgg ttgccgatca
     4981 cgtggacctc gctggtggtc agcggtgccg gggtgtcgaa catcgcgagc ccggtctggg
     5041 ccgggccgta cggcgccggc gcctgggtga acagctcgtc ccaggtcagc ccggcggccg
     5101 gcaggatcac gaccgtgttc accgggatcg tcacgacctg accgttgacc cgcagcgtgc
     5161 cgggcgcatc cggccccgcg gtgtgggctt cctggagaaa gccggtgatg tcgaactgcg
     5221 tgctcgtcgg gatcggcagc gggaccccgg ccgccgccgc ggccgcgaat cgccgcgccg
     5281 cgcccggcgc cggg
//
LOCUS       DASVOR010000085         4228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_117844,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000085 DASVOR010000000
VERSION     DASVOR010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4228
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117844"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF516_01820"
     CDS             complement(<1..272)
                     /locus_tag="VF516_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8106428.1"
                     /translation="MYVPLLDRLLYRHPFEGRKARRYERDARPAFGDFDDRLLDRLAP
                     RLDGAGDAGDARRLLDLGCGPGTFARRAAARFAALHVVAIDPSRDFA"
     gene            374..2374
                     /locus_tag="VF516_01825"
     CDS             374..2374
                     /locus_tag="VF516_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106429.1"
                     /translation="MRHRTAVRSIAVSLGLTLGAGIAACGDDVTASPGLAPDCNPLGG
                     QGCLLPWPSMAYLRADAATPSGYRVDLPAGGMPVNASGVPVDPAPLARWDGFSPTGPI
                     LAMFPRGVSPGGLPSFKSPDDSLAPGSPIVLYDLDRGERAPFFAEVDQNVVDVNQRAL
                     VIRPLARLHERTHYAVAIRNTVKSADGTPLPVSPGFAALRDGKPLDHPRFAQLAAGAP
                     AMFDRLAAAGIDRAELVLAWDFRTASDEMLRRDLTAMRAAALPAIGDRGAGLGTSPFT
                     VEDQPNTPQSYRRFIGTYKSPDFLTAGETADSILARDAAGAPELHGLRDAKFAAIIPR
                     CVETQPLPRPTIIFGHGLFGSSADYLSDGFVQQLAEDHCLVIVAGDFIGLTSRDLVTA
                     TNAVIDLNRGPGVTEKLAQSVIDFIALESLTRGALAAAPEFRIGGTAGGAAVIDPANT
                     YYIGGSLGGIMGNVIMAYDPNLTRGVLAVPGGAWSLLIERSNAWVVLKALIQSAYADP
                     QVYPLDVALFGMAFEPYDPITTAAHVLHDPLFGNPVKDILMWYSIGDCLVSNLTTELT
                     ARTMGIGMIGPSVKAPWGLAPTPGAFASGIVVFDDHPTPLPPDTNEPPATDNGTHSGI
                     NRKPAALRMVQRFLLPPDRQAADGCTLAGSPAPCDCATGACD"
     gene            2468..3451
                     /locus_tag="VF516_01830"
     CDS             2468..3451
                     /locus_tag="VF516_01830"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEX8106430.1"
                     /translation="MPRHLVSIADLDAAEIVRILDHGDRYTALASEPIKKLTSLRGRT
                     VINLFFEASTRTRTSFEIAAKRLSAEAINISSSASSISKGETLLDTAENLEAMSPDVI
                     VVRHSFGGAAAMLAEHVSCAVINAGDGQHEHPTQALLDAATIRRIKGKRAAPGGPASI
                     EGLEVAIVGDIAHSRVARSNVLCLAKLGARIRLVAPLTLIPRGIELIGGDVTRERVRL
                     VTRLEDGLDGVDVVMMLRIQHERVGGEAPRFPNTRELSRMFGLSERTLGFAKSDAIVM
                     HPGPINRGVELAPQVADSPRAVILQQVSWGVAIRMAVLERAVIGFEDRAAA"
     gene            3448..>4228
                     /locus_tag="VF516_01835"
     CDS             3448..>4228
                     /locus_tag="VF516_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8106431.1"
                     /translation="MSHALVVRGLVKRFGNHVAVDDISFEVPRGVVYGILGPNGAGKS
                     TTLRMINDIFAPDAGEIVVLDGLPPGHAAAQHIGYLPEERGLYPKMKVADMIEFIGEL
                     RGLARGEARRRTERWLDRLGLAQWAGNKIQDLSKGMQQKVQFATALIHEPELVILDEP
                     WSGLDPINAEVLREVVEDIRRNGRTVLFSTHQMEQAEKLVDHICIIARGRKLLDGSLA
                     DIQRREAADGIISLGLALGPEDAASRAAADAVLGDRALVAER"
BASE COUNT          600 a         1579 c         1455 g          594 t
ORIGIN      
        1 gcgaagtcgc ggctggggtc gatcgccacg acgtgcagcg ccgcgaaccg cgccgccgcc
       61 cgccgcgcga acgtccccgg cccgcagccg agatcgagca gccgtcgtgc atcgccggcg
      121 tcgccggcgc cgtcgagccg gggcgccagc cggtcgagca gccggtcgtc gaagtcgccg
      181 aacgccggcc gggcgtcgcg ctcgtagcgc cgggccttgc gtccttcgaa tggatgccgg
      241 tacaggagcc ggtcgagcaa cgggacgtac acgcgatcag tcgatgtata acgcatcggt
      301 gcagttcgcc catatcccat ctccggcgag gcagcgatgc cggagaatgc cggtgatatc
      361 gttgtccggc tgtatgaggc accgcacagc cgtccgctcc atcgctgtca gcctcggtct
      421 caccctcggc gcggggatcg ccgcatgtgg tgacgacgtc accgcgtcgc cggggctcgc
      481 gccggactgc aacccgctcg gcggccaggg ctgcctgctg ccgtggccgt cgatggcgta
      541 cctccgcgcg gacgccgcga cgccgagcgg gtaccgggtc gacctgccgg ccggcggcat
      601 gccggtcaac gcctccggcg tgccggtcga tcccgcgccg ctcgcgcggt gggacgggtt
      661 ctcgccgacc ggcccgatcc tggcgatgtt cccgcgcggg gtgtcacccg gcgggctgcc
      721 gtcgttcaag agcccggacg actcgctcgc gccgggctcg ccgatcgtgc tctacgacct
      781 cgaccgcggc gagcgcgcgc cgttcttcgc cgaggtcgac cagaacgtcg tcgacgtgaa
      841 ccagcgcgcg ctggtgatcc ggccgctcgc gcggctgcac gagcgcacgc actatgcggt
      901 cgcgatccgc aacacggtca agagcgccga cggcacgccg ctgccggtgt cgccggggtt
      961 cgccgcgctg cgcgatggca agccgctcga ccacccgcgg ttcgcacagc tcgccgccgg
     1021 cgcgcccgcg atgttcgatc ggctcgccgc cgccggcatc gaccgcgccg agctcgtgct
     1081 ggcctgggat ttccggaccg cgtccgacga gatgctgcgc cgcgatctga ccgcgatgcg
     1141 cgccgccgcg ctgccggcga tcggcgaccg cggcgccggc ctcggcacgt cgccgttcac
     1201 cgtcgaggat cagcccaaca cgccgcagag ctaccggcgg ttcatcggga cctacaagtc
     1261 gccggacttc ctgaccgccg gcgagaccgc cgattcgatc ctcgcgcgcg acgccgccgg
     1321 cgcacccgag ctgcacggcc tgcgcgacgc gaagttcgcc gcgatcatcc ccaggtgcgt
     1381 cgagacccag ccgctgccgc ggccgacgat catcttcggc cacggcctgt tcggctcgtc
     1441 ggccgactac ctgagcgacg gcttcgtcca gcagctcgcc gaggaccact gcctggtgat
     1501 cgtcgccggc gacttcatcg gcctgacctc gcgcgacctg gtcaccgcga ccaacgcggt
     1561 catcgatctc aaccgcggcc ccggggtcac cgagaagctc gcccagtcgg tgatcgactt
     1621 catcgcgctg gagtcgctga cccgcggcgc gctggcggcc gcgcccgagt tccggatcgg
     1681 cggcacggcc ggcggcgcgg cggtgatcga ccccgcgaac acgtactaca tcggcggctc
     1741 gctgggcggg atcatgggca acgtgatcat ggcctacgat ccgaacctga cccgcggcgt
     1801 gctcgcggtg ccgggcggcg cgtggtcgct gctgatcgag cgctcgaacg cgtgggtcgt
     1861 gctcaaggcc ctgatccaga gcgcctacgc cgacccgcag gtctacccgc tcgacgtcgc
     1921 gctgttcggc atggcgttcg agccctacga tccgatcacg accgccgccc acgtcctcca
     1981 cgacccgctg ttcggcaacc cggtcaagga catcctgatg tggtactcga tcggcgactg
     2041 cctggtctcg aacctcacca ccgagctgac cgcgcgcacc atggggatcg ggatgatcgg
     2101 accgtcggtc aaggcgccgt ggggcctggc cccgaccccg ggggcgttcg ccagcggcat
     2161 cgtcgtgttc gatgaccacc cgacgccgct gccgcccgac accaacgagc cgccggcgac
     2221 cgacaacggc acccattccg ggatcaaccg caagcccgcc gccctgcgca tggtccagcg
     2281 cttcctcttg ccacccgacc gccaggccgc cgacggctgc acgctcgccg gcagcccggc
     2341 accctgcgac tgtgccaccg gcgcctgcga ctgacgccga tcccctcgat cgtacgccct
     2401 cgatcttgcg cctcgatctg ccggcctgcc gccgcaccgg cggtggcggt tctgctatat
     2461 gaaaggcgtg cctcgccacc tcgtcagcat cgccgacctc gatgctgcgg agatcgtgcg
     2521 gatcctcgac cacggcgacc gctacacggc gctggccagc gagccgatca agaagctgac
     2581 gtcgctgcgc ggccgcaccg tgatcaacct gttcttcgag gcctcgaccc ggacccggac
     2641 gtcgttcgag atcgccgcca agcggctgtc ggccgaggcg atcaacatct cgagctcggc
     2701 gtcgtcgatc tccaagggcg agaccttgct cgacaccgcc gagaacctcg aggcgatgtc
     2761 gcccgacgtg atcgtggtcc ggcactcgtt cggcggcgcg gcggcgatgc tggccgagca
     2821 cgtcagctgc gcggtgatca acgcgggcga cggccagcac gagcacccga cccaggcgct
     2881 gctcgacgcc gcgacgatcc gccggatcaa gggcaagcgg gccgcgcccg gagggccggc
     2941 gtcgatcgaa gggctcgagg tcgcgatcgt cggcgacatc gcgcactcgc gggtcgcgcg
     3001 ctcgaacgtg ctgtgcctgg ccaagctcgg cgcccggatc cggctggtcg cgccgctcac
     3061 gctgatcccg cgcggcatcg agctgatcgg cggcgacgtg acccgcgagc gggtccggct
     3121 ggtcacccgg ctggaggacg ggctcgacgg cgtcgacgtc gtgatgatgc tgcggatcca
     3181 gcacgagcgg gtaggcggcg aggcgccgcg gttccccaac acccgcgagc tgtcgcgcat
     3241 gttcgggctc agcgagcgta cgctcgggtt cgccaagtcc gacgcgatcg tcatgcaccc
     3301 cgggccgatc aaccgcggcg tcgagctcgc accgcaggtc gccgacagcc cgcgggcggt
     3361 gatcctgcag caggtcagct ggggcgtcgc gatccgcatg gccgtgctgg agcgcgcggt
     3421 gatcgggttc gaagatcggg cagccgcatg agccacgcgc tggtcgtccg cggcctggtc
     3481 aagcggttcg gcaaccacgt cgccgtcgac gacatctcgt tcgaggtccc gcgcggcgtg
     3541 gtgtacggca tcctcgggcc caacggcgcc ggcaagtcga ccacgctgcg catgatcaac
     3601 gacatcttcg cgcccgacgc cggcgagatc gtcgtgctcg acggccttcc gcccggccac
     3661 gccgccgcgc agcacatcgg ctacctgccc gaggagcgcg gcctgtaccc caagatgaag
     3721 gtcgccgaca tgatcgagtt catcggcgag ctgcgcggcc ttgcccgcgg cgaggcgcgg
     3781 cggcggaccg agcgctggct cgaccggctc ggcctggccc agtgggcggg caacaagatc
     3841 caggacctgt ccaagggcat gcagcagaag gtccagttcg cgaccgcgct gatccacgag
     3901 cccgagctgg tgatcctcga cgagccgtgg agcgggctcg atccgatcaa cgccgaggtg
     3961 ctgcgcgagg tggtcgagga catccgccgg aacggccgca ccgtgctgtt ctcgacccac
     4021 cagatggagc aggccgagaa gctggtcgat cacatctgca tcatcgcgcg cggcaggaag
     4081 ctgctcgacg gctcgctcgc ggacatccag cgccgcgagg cggccgacgg catcatctcg
     4141 ctgggcctcg cgctcggccc cgaagacgcc gcgtcgcgcg ccgcggccga cgccgtgctc
     4201 ggcgatcgcg cgctggtcgc cgagcgac
//
LOCUS       DASVOR010000086        11526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439185,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000086 DASVOR010000000
VERSION     DASVOR010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11526
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439185"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2172
                     /locus_tag="VF516_01840"
     CDS             <1..2172
                     /locus_tag="VF516_01840"
                     /inference="COORDINATES: protein motif:HMM:NF019298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX8106432.1"
                     /translation="DDDGDALDEQGWAPVRVFPVPQYTQPYDGPRTDFRETLYWNGNV
                     QTGADGNAEVAFVASDAITSFRATAEGVSAGGLAGRGSLTFQSKMPMTLDAHLPLEVT
                     SGDEIRLPVTLANETDRALDAALDARFGAAFQLTASPITGAIHLAAGQKQTVLFPLRV
                     VATDGAGDVDLALTTAGLKDQLHKQIRVVPLGFPFEVAASGTAKSGAPARHDFDLAGA
                     LPGSIRATVTMYPSPLAAMTKGMEAMIREPGGCFEQTSSTNYPNIMILGYLGANDAAD
                     PALLAKTQGTLDRGYKLLTGYETKQKGYEWFGQTPGHEALTAYGLMEFADMAKVYDVD
                     RQMVERTADWLMTRRDHKGGFLRSAEALDSFGRAGEATTNAYIVWALAEAHRTAGLDP
                     ELAVQRTLGADTRDPYLLTLATNTGLLAAPRAGETAAMVRRLVAMQAKDGSFPGAKES
                     ITMSGGESLTVETTALATLALIKASPASEYEPQIRAAVDWLNARRGGYGQWSNTQATV
                     LGLKALTAYADHSRQMQAAGSATLIVNGKPAGTIAFDKGRKDALVWDDLATALRPGPN
                     TLELQLAGGATLPYSIAIEYRSARPQSSPAAKVAVTTQLAKTQVHMGEGVKLRAHVEN
                     TSSGGVPMTLARVGIPGGLTFQTWQLKELRDKGAIDFYETRPREVILYWRALPPSAKK
                     DIDLDLLAAVPGHYEAPATSAYLYYTAEDKAWTAPVQVTVD"
     gene            2648..8032
                     /locus_tag="VF516_01845"
     CDS             2648..8032
                     /locus_tag="VF516_01845"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8106433.1"
                     /translation="MSERADKQSKQRSVQTALGTVIATPGKRTAVEQAYVQRQVVESC
                     NEQVHASEAGSAPAPALLPGDRIQRLFGRPDSQIASRHVNQPSPEPPVQRKATGDAES
                     GADASAVAAEATGGPGGALPHQAQIQTSFGRHDVSNIRGFQGSETHGAMQTLGARAFA
                     YGDAVAFHGAPDLHTAAHEAAHVVQQRSGVQLKGNIDQPGDVYERHADAVADRVVAGQ
                     SAEALLDQMAGGSGASSSVQRKPDDKQPAKDDVPHLPDGMNLQIQSDGSVLVRAAWVE
                     SAPDKVVSTQGVQSPSVISAILHALKDAGILYWIPDAKIKEYATSLVLEGSYNSKVKP
                     FNKLGIAFSVYVRIGPPPGVNVIYAKSGRALQGIIRQSALIPGPIVAETEVELSEPVR
                     NELWTALEQFTGMPLAPEVKADLVKNPEPWKPHVLPGQEGLAFPLEQKTLERLYGKKQ
                     WNDHLDHPKSDQQGSGAITVAGGVQFSSTIPEEDRKYFLDWMKQVAKGGKPGDTLITS
                     DVIAKLREIDADPDLKAKVLARLQAGGNGGESKPLTAQMLDNVITAVKTDDAYKKLGM
                     TPPTGGDRYKPLFSEPVRGKIINRGGLNYVGQDTEFSFETQNQKDAFAIPYVYVNWVL
                     TKKDKPTEHLKEGKTHHVEFEKPDTFAYEWKETGICTVHAFVTHTFYQPAHFEIDVEV
                     KTEQQRTKELNDAAFKGLQGGTTYETGPTDWPFDVSWFNTAFGSRKEEYGKKVWGTTP
                     ADFKRMTYEERVKFLATDKQRLKDMIAAHTNAKGKPTDARWADMVSYAKDKLKTLEET
                     EQTLGKENTAGSVFFEARGTFLSRKNGIPDKALKLLGSAKKDSADIKTTVHDFTQLYE
                     PNDYTFSATGTTFEKSVEATFVSLAKSYPPGRISCLFEALDDHLAPTQKTVGFEFDTG
                     TAWKDVKSVVYNPTVMMVVNIVGAATMVFLPVTAPVLFPMLATYNSIETIDNMAQLQA
                     KGNLTWGNVAKGVAQIGLNFLPYVGELKVVANMGKVAMYTLEGVTIAGMGVLMTLDGV
                     EQIRKLRDKDISEIGKLDEEVRELERTNPSDPTLPAKKAQLEAMIRQAQDRSTEVIQD
                     MAKSGAVMLVQMAALKGLQAHMTNASIASLKAEGLYEHAPGVEPHYDAKTGRIVGDEA
                     KLDPGKISKLKQQYTVDMAAKQAELANVLGTEKIEVTRGGDKITVTKDGDGYKVQIPN
                     DKPFSEAIDEAWKARKAMDPKAPADRPPPIVAEIKPTLDPRALVHEQNVAVGTSVQTE
                     TEAHGILDKLAKGDRSAFKALGIEAPPADFDLRSVEWGIGQKPDGSFIIIRGEPGAVN
                     WSGFGGVRPVAHSHPLIPQKLLTGGSVTFAELIKGGAKMEVNKVNVFPSAADVQFCVA
                     HGLTEHTVQTPYVSKGGGKIGNPAPSAHEPLVSIKIKQPERVGSWGGNDQLGVYKSKM
                     AAVDANGNELWSGEVWTADHPSLGSVVMFDEPPSGLMTKKVAGAAPGVPHSTKTSDPA
                     VTRWVSDLPGQLSRDEQHRLDLMTKGKSADEIYDLFGGDLAVAKAKLGGSNLTPELYQ
                     KRASLTSDKARRAFDNKWNQMIGGDRAPSDAKIAKFKGYLDAIEKRAGGDLNKGLEAE
                     EKKIPHGAVVAEPKAADYPPSWDNFDPAHNAEFKTKLEEFRGTDFMEQNYAGGEGAVY
                     LGESKTTALKRWFAKRIGDMATSIAKLRSARSAVTGNPDLAKHIDVVEIHETGSDWIV
                     RDFATGSDELKNFASGPAEIARTEAIKALEAKPSRSATEDDILGKLKGKSANLHWEPA
                     RGKIVIIDMQ"
     gene            8047..8586
                     /locus_tag="VF516_01850"
     CDS             8047..8586
                     /locus_tag="VF516_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106434.1"
                     /translation="MRTFAELHRSERLHHARDTELGAEYWMADGGPRVRVFEHVGPAG
                     LAAYVTLHEYAHLWIHGDYDHDAWLVLDVDEPIEVGVDFVVMPRRIGNTLDSFVRDED
                     RAEPPPRLADVRALIPKLVAAAVTPADRWVAALVANRLAGPDIHTIFMWAELRFYIQD
                     LAPSREELDAWRAVAPPQG"
     gene            complement(8601..9086)
                     /locus_tag="VF516_01855"
     CDS             complement(8601..9086)
                     /locus_tag="VF516_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEX8106435.1"
                     /translation="MSGSGSDVVLVPVAGGALALTHRPKLKAIPALRGLGATHLVTLL
                     AEREGAKQIGNAATAAGLTWLWTPLDGGDVPDAARTAELRPRLAEIAAAIARGGKVVV
                     HCSAGVHRTGMIGYALLRQLGLSADAAREKLSALRSVTAEGVGSDRLAWGDSLAGGEP
                     G"
     gene            9119..10228
                     /locus_tag="VF516_01860"
     CDS             9119..10228
                     /locus_tag="VF516_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106436.1"
                     /translation="MSEPRVESVSEGSVVVIPDPDGVLGALIAALLAEPGEMRLKAIA
                     HGTVDTLSLGELELRCSTTRGGVAPTWSVNRGSATAIVTGDVVERLVDQAYQHRRAAE
                     LATFPIGGRLGQGRYVITHALRGGPDRGMYRARAAGEVTTYLATLGPPQTRDFSELRR
                     ELAFDVPGIAPLEYIGRLEPHSEAGHEGMLEREPAGLPAAELAHPMARDATLQLALGI
                     ARVIRGAHAAGIFLGTLRPELVYVQSAAVPVVTGIAPRAERFWLTATPRTYGVAPCFD
                     DFYQSPEQLAQPHDPATAASDVFALGAMLAHWLAGEHPFEGEGSLQAMSIALGRRRAF
                     AGPPELTAPIDGALAPASERVSLDAWIRELETLAR"
     gene            complement(10245..>11526)
                     /locus_tag="VF516_01865"
     CDS             complement(10245..>11526)
                     /locus_tag="VF516_01865"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8106437.1"
                     /translation="IAMMSGITDKIESGEDCLYLNVFAPAAPGPHPVMVWIHGGAFVM
                     GSGSTPLYHGETFARQGIVTVTVNYRLGLPGLLFLGDLAPGHDAGDCFLLDQVAALRW
                     VKDHIAAFGGDPGQVTVMGESAGAISISMLLAMPAARGLFHRVILESGAPSLGPPTRA
                     DATEVSRWVLADLGATPEALADVPIDRLLAAQERRAQTHGLGAYAPYIDGVTVPRLPI
                     DAIRAGAAAGIPILLGTNRDEWNLFEVFFGAATVEPFKPLVRDRLGPLTDSLVELYRD
                     GHPDRSPKRAWVDLVGELVFRIPAIRLAEAQSARGAPVYMYRFDWKSPTFGGRLGAAH
                     ALELPFVWNRLDLPMTPVLLGPDAAPLQPLATAMHGAWCQFIRAGDPNGGGLPSWPRY
                     DARRRATLLIDRESHVADDPGGAARAAWPAVWPG"
BASE COUNT         2006 a         4081 c         3946 g         1493 t
ORIGIN      
        1 gacgacgatg gcgacgccct cgacgagcag ggctgggcgc cggtccgcgt gttcccggtc
       61 ccgcagtaca cccagccgta cgacggcccg cgcaccgact tccgcgagac cctctactgg
      121 aacggcaacg tccagaccgg cgccgacggc aacgccgagg tcgcgttcgt cgcgtccgac
      181 gcgatcacct cgttccgcgc caccgccgag ggcgtgtcgg cgggcggcct cgccggccgc
      241 ggcagcctga ccttccagtc gaagatgccg atgacgctcg acgcccacct gccgctcgag
      301 gtcacctcgg gcgacgagat ccgcctgccg gtcacgctcg ccaacgagac cgaccgcgcg
      361 ctcgacgccg cgctcgacgc ccggttcggc gccgccttcc agctcaccgc cagcccgatc
      421 accggcgcga tccacctcgc cgccggccag aagcagaccg tgctgttccc gctccgcgtc
      481 gtcgcgaccg acggcgccgg cgacgtcgac ctcgcgctga ccaccgccgg cctcaaggac
      541 cagctccaca agcagatccg cgtcgtcccg ctcggcttcc cgttcgaggt cgccgcctcg
      601 ggcaccgcga agtccggcgc ccccgcgcgc cacgacttcg acctcgccgg cgcgctgccc
      661 ggctcgatcc gcgccaccgt caccatgtac ccgtcgccgc tcgccgcgat gaccaagggc
      721 atggaggcga tgatccgcga gccgggcggc tgcttcgagc agacctcgtc gaccaactac
      781 cccaacatca tgatcctcgg ctacctcggc gccaacgacg ccgccgaccc cgccctgctc
      841 gccaagaccc agggcaccct cgatcgcggc tacaagctcc tcaccggcta cgagaccaag
      901 cagaagggct acgagtggtt cggccagacc cccggccacg aggcgctcac cgcctacggc
      961 ctcatggagt tcgccgacat ggccaaggtc tacgacgtcg atcgccagat ggtcgagcgc
     1021 accgccgact ggctcatgac ccgccgcgat cacaagggcg gctttttacg cagcgccgag
     1081 gcgctcgact cgttcggccg cgccggcgag gcgaccacca acgcgtacat cgtgtgggcg
     1141 ctcgccgagg cccaccgcac ggccggcctc gaccccgagc tcgccgtcca gcgcaccctc
     1201 ggcgccgaca cccgcgaccc ctacctgctc acgctcgcga ccaacaccgg cctgctcgcc
     1261 gccccgcgcg ccggcgagac cgccgccatg gtccgccgcc tcgtcgccat gcaggccaag
     1321 gacggcagct tcccgggcgc caaggagtcg atcaccatgt ccggcggcga gtcgctcacc
     1381 gtcgagacca ccgcgctcgc caccctcgcg ctgatcaagg cgtcgccggc cagcgagtac
     1441 gagccccaga tccgcgccgc cgtcgactgg ctcaacgccc ggcgcggcgg ctacggccag
     1501 tggagcaaca cccaggccac cgtcctcggc ctcaaggcgc tcaccgccta cgccgaccat
     1561 tcccgccaga tgcaggcggc cggctccgcg accctgatcg tcaacggcaa gcccgcgggc
     1621 accatcgctt tcgacaaggg ccgcaaggac gccctggtct gggacgacct cgcgacggcg
     1681 ctccgccccg gtcccaacac cctcgagctc cagctcgccg gcggtgccac cctgccctac
     1741 tcgatcgcca tcgagtaccg ctcggcgcgc ccgcagtcct cccccgccgc caaggtcgcc
     1801 gtcaccaccc agctcgcgaa gacccaggtc cacatgggcg aaggcgtcaa gctgcgcgcc
     1861 cacgtcgaga acacctcgtc gggcggcgtg ccgatgaccc tcgcccgcgt cggcatcccc
     1921 ggcggcctga cgttccagac ctggcagctc aaggagctgc gcgacaaggg cgcgatcgac
     1981 ttctacgaga cccgcccgcg cgaggtcatc ctctactggc gcgcgctgcc gccgtcggcc
     2041 aagaaggaca tcgacctcga cctgctcgcc gccgtccccg gccactacga ggccccggcg
     2101 acctcggcgt acctctacta caccgccgag gacaaggcct ggacggcccc ggtccaggtc
     2161 accgtcgact gacggatcga catggcctcg gctcctccgg cgccggtgtc gtggagcgca
     2221 cgctcgacct gcgagcggat cgctgccagc gctcgccggg cgagatcctc gcacgcggcc
     2281 gcgtcccaat gagtacgaac ccgacttgga aacatctgtc acctgggccg cgcaacgttg
     2341 gctcggcaga ttctgcagcg catcgatcac cggcgtgaac gccggaccct cgcgcgcgag
     2401 gcagagcgtg aacgcaagcg caggtcgccg cgctgagcgc cgcgtcggtg gccgcaagct
     2461 cggcggcggg tgctgtcggt cgagccgcca gaacccgagt tggcaccccg ccattgtcgg
     2521 tgacgacatt tcctcgcatg tgcggcgacg tggttaccag ctcattcacc gaggccgaca
     2581 ctccggatga ttgacatacc tggcagcgtt cgacggacac ggatcacaat acagtgggcg
     2641 agtagcgatg tccgaacgtg cggacaagca gtcgaagcag cgcagtgtgc aaactgcgct
     2701 cggtacggtc atcgcgacgc ccggaaaacg taccgcggtc gagcaggcgt acgtgcagcg
     2761 gcaggtggtc gagagctgca acgagcaggt tcacgcgtcc gaagcgggta gcgcgccagc
     2821 gccggctttg ttgccaggcg atcggatcca gcggctattc ggtcgcccag acagccagat
     2881 tgcgagccgc catgtcaacc agccgtcgcc cgagccgccg gtccagcgca aggccaccgg
     2941 cgacgccgag tccggcgccg atgcatcggc ggtcgccgcc gaggccacgg gcggcccggg
     3001 cggtgcgctg ccgcaccagg cgcagatcca gaccagcttc ggtcgccacg acgtctcgaa
     3061 catccgcggg ttccagggca gcgagacaca cggcgcgatg cagaccctcg gcgcccgcgc
     3121 gttcgcgtac ggcgatgcgg tcgcgttcca cggcgcaccc gacctgcaca ccgcggcgca
     3181 cgaggccgcg cacgtcgtcc agcagcgcag cggcgtccag ctgaagggca acatcgacca
     3241 gcccggcgac gtctacgagc gccacgccga cgcggtcgcc gatcgcgtcg tcgcgggcca
     3301 gtcggccgag gcgctgctcg atcagatggc gggcggcagc ggggcgagtt cctcggtgca
     3361 acgcaagccg gacgacaagc agccggccaa ggacgacgtc ccgcatctgc cggacgggat
     3421 gaatctgcag atccagtcgg atggctcggt gctggtgcgt gccgcgtggg tcgagtcggc
     3481 gcccgacaag gtcgtctcga cccagggcgt ccagtcaccg agcgtgatct ccgcgatcct
     3541 gcacgcgctc aaggacgcgg gcatcctgta ctggataccc gacgccaaga tcaaggagta
     3601 cgcgacgagc ttggtgctcg agggctcgta caactccaag gtcaagccgt tcaacaagct
     3661 cggcatcgcg ttcagcgtct acgtgcggat cggcccgccg cccggcgtca acgtcatcta
     3721 cgccaagagc ggccgcgccc tgcagggcat catccgccag tcggcgctga tccccggtcc
     3781 gatcgtggcg gagaccgagg tcgagctcag cgagccagtg cgcaacgagc tgtggactgc
     3841 actcgagcag ttcaccggga tgccgctcgc ccccgaggtg aaggcggatc tggtcaagaa
     3901 ccccgagccg tggaagccgc acgtcctgcc cggccaggag ggcctcgcgt tcccgctcga
     3961 gcagaagacg ctcgagcggc tgtacggcaa gaagcaatgg aacgaccacc tcgatcatcc
     4021 caagtcggac cagcagggct cgggggcgat cacggtcgcg ggcggcgtac agttctcgtc
     4081 gacgatcccc gaggaggacc gcaagtactt cctcgactgg atgaagcagg tcgcgaaggg
     4141 cggcaagccc ggtgacacgc tgatcacgtc cgacgtgatc gcgaagctgc gcgagatcga
     4201 cgccgatccg gacctcaagg ccaaggtgct cgcgagactc caggccggcg ggaacggcgg
     4261 cgagagcaag ccgctgaccg cgcagatgct cgacaacgtg atcaccgcgg tcaagaccga
     4321 cgacgcctac aagaagctcg gcatgacgcc gccgacgggt ggcgacaggt acaagccgct
     4381 gttctccgag ccggtgcgcg gcaagatcat caaccgcggt gggctcaact acgtcggcca
     4441 ggacaccgag ttctcgttcg agacccagaa ccagaaggac gcattcgcga tcccgtacgt
     4501 ctacgtgaac tgggtcctga ccaagaagga caaaccgacc gagcacctca aggagggcaa
     4561 gacccaccac gtcgagttcg agaagcccga cacgttcgcg tacgagtgga aggagaccgg
     4621 catctgcacg gttcacgcgt tcgtgacgca caccttctac cagccggcgc acttcgagat
     4681 tgatgtcgag gtcaagaccg agcagcagcg gaccaaagag ctcaacgacg ctgcgttcaa
     4741 gggcctgcag ggcggcacca cgtacgagac cgggcctacc gactggccgt tcgacgtgtc
     4801 gtggttcaac acggcgttcg gcagccgcaa ggaggagtac ggcaagaagg tctggggcac
     4861 gacgcccgcc gacttcaagc gcatgaccta cgaggagcgc gtcaagttcc tggcgaccga
     4921 caagcagcgc ctgaaggaca tgatcgcggc gcacaccaac gccaagggca agccgaccga
     4981 cgcgcggtgg gccgacatgg tgtcgtacgc caaggacaag ctgaagacgc tcgaggagac
     5041 cgagcaaacg ctgggcaagg agaacaccgc aggcagcgtg ttcttcgagg cccgcggcac
     5101 attcctgtcg cgcaagaacg gcatccccga caaggcgctc aagctgctcg gctcggcgaa
     5161 gaaggacagc gccgacatca agaccaccgt ccacgacttc acacagctct acgaaccgaa
     5221 cgactacacg ttctcggcga ccggcaccac gttcgagaag tcggtcgagg cgacgttcgt
     5281 cagcctggcg aagtcatacc cgccgggccg gatctcgtgc ctgttcgagg cactcgacga
     5341 tcacctcgcc cccacccaga agaccgtcgg gttcgagttt gacaccggca cggcgtggaa
     5401 ggatgtcaag agcgtcgtct acaacccgac cgtcatgatg gtggtgaaca tcgtcggcgc
     5461 ggcgacgatg gtgttcctgc cggtgaccgc tccggtgctg ttcccgatgc tcgccaccta
     5521 taactcgatc gagacgatcg acaacatggc gcagctgcag gccaagggga acctgacctg
     5581 gggcaacgtc gccaagggcg tggcgcagat cggcctcaac ttcttgccgt acgtcggcga
     5641 gctcaaggtc gtcgcgaaca tgggcaaggt ggcgatgtac acgctcgagg gcgtgaccat
     5701 cgcgggcatg ggcgtgctga tgaccctcga cggcgtggag cagatccgca agctgcgcga
     5761 caaggacatc agcgagatcg gcaagctcga tgaggaggtc cgcgagctcg agcgcaccaa
     5821 cccgtccgac ccgacgctgc cggccaagaa ggcgcagctc gaggcgatga tcaggcaggc
     5881 ccaggatcgc tcgaccgagg tgatccagga catggcgaag tcgggcgccg tcatgctggt
     5941 ccagatggcc gcgctcaagg gcctgcaggc ccacatgacg aacgcgtcga tcgcgagcct
     6001 caaggccgag ggcctctacg agcatgcgcc gggcgtcgag ccgcactatg acgccaagac
     6061 cggccggatc gtcggtgacg aggccaagct cgacccaggc aagatcagca agctcaagca
     6121 gcagtacacg gtcgacatgg ccgccaagca ggccgagctc gcgaacgtgc tcggcaccga
     6181 aaagatcgag gtcacgcgcg gcggcgacaa gatcacggtc accaaggacg gcgacggcta
     6241 caaggtccag atcccgaacg acaagccgtt ctccgaggcg atcgacgagg cgtggaaggc
     6301 ccgcaaggcc atggatccca aggcgccggc cgatcgcccg ccgccgatcg tcgccgagat
     6361 caagccgacg ctcgatccca gggcgctggt ccacgagcag aacgtcgcgg tcggtacctc
     6421 ggtgcagacc gagaccgagg cgcacgggat cctcgacaag ctcgccaagg gcgatcgctc
     6481 ggcgttcaag gcgctcggca tcgaggcgcc gcccgccgac ttcgacctgc gcagcgtcga
     6541 gtggggcatc ggccagaagc ccgacggctc gttcatcatc attcgtggcg agcccggcgc
     6601 cgtgaactgg tcgggcttcg gcggcgtgcg cccggtcgcc cacagccatc cgctgatccc
     6661 gcagaagctc ctgaccggcg gcagcgtgac gttcgccgaa ctgatcaagg gcggggcgaa
     6721 gatggaggtc aacaaggtca acgtgtttcc gtcggcggcc gacgtgcagt tctgcgtcgc
     6781 gcacggcctg accgagcaca ccgtccagac tccgtacgtc agcaagggcg gcggcaagat
     6841 cggcaacccg gccccgagcg cgcacgagcc gctggtctcg atcaagatca agcagcccga
     6901 gcgcgtcggc tcgtggggcg gcaacgatca gctcggcgtc tacaagtcga agatggccgc
     6961 cgtcgatgcg aacggcaacg agctgtggag cggcgaggta tggaccgccg accatccgag
     7021 cctcggcagc gtcgtgatgt tcgacgagcc gccgagcggc ctcatgacga agaaggtcgc
     7081 tggcgccgcc ccgggcgtcc cgcactcgac gaagaccagc gatccggccg tgaccaggtg
     7141 ggtcagcgat ctgccgggcc agctatcgcg cgacgagcag caccggctcg acctgatgac
     7201 caagggcaag agcgccgacg agatctacga cttgttcggc ggtgacctcg cggtcgccaa
     7261 ggccaagctc ggcggctcga acctgacacc ggagctgtac cagaagcgcg cgagtctgac
     7321 ctccgacaag gcgcggcgcg cgttcgacaa caagtggaac cagatgatcg gcggcgatcg
     7381 cgcgccctcg gacgcgaaga tcgcgaagtt caagggctac ctggacgcga tcgagaaacg
     7441 cgccggcggc gacctcaaca agggcctcga ggccgaggag aagaagatcc cgcacggtgc
     7501 cgtggtggcc gagccgaagg ccgcggacta cccgccgtca tgggataact tcgaccccgc
     7561 gcacaacgcc gagttcaaga ccaagctcga ggagttccgc ggcaccgact tcatggagca
     7621 gaactacgcc ggcggtgagg gcgcggtgta cctcggcgag agcaagacca cggcgctcaa
     7681 gcgctggttc gcgaaacgga tcggagacat ggcgacgtcg atcgcgaagc tgcgcagcgc
     7741 acgctcggcc gtgaccggga acccggacct cgccaagcac atcgacgtcg tggagatcca
     7801 cgagaccgga agcgactgga tcgtgcgcga cttcgcaacc ggctccgacg agctcaagaa
     7861 ctttgcctcg ggtccggccg agatcgcgcg caccgaggcc atcaaggcgc tcgaggccaa
     7921 gccgtcgcgg tctgcgaccg aggacgacat cctcggcaag ctcaagggca agtcggcgaa
     7981 cctgcactgg gagccggccc gcggcaagat cgtcatcatc gacatgcagt aagctgacgc
     8041 ggacgcatgc ggacctttgc cgagctgcac cgctccgagc gcctccacca cgcacgcgac
     8101 accgagctcg gtgccgagta ctggatggcg gacggtggac cgcgcgtccg cgtcttcgag
     8161 cacgtcgggc ccgcgggcct ggccgcgtac gtcacgctcc acgagtacgc gcatctgtgg
     8221 atccacggcg actacgacca cgacgcgtgg ctcgtgctgg acgtcgacga gccgatcgag
     8281 gtcggcgtcg acttcgtggt gatgccgcgc cggatcggca acacgctcga cagctttgtg
     8341 cgcgacgagg atcgcgccga accgccgccg cgcctcgccg acgtgcgcgc gctgatcccc
     8401 aagctcgtcg ccgccgcggt gacgccggcc gaccgctggg ttgcggccct ggtcgcgaac
     8461 cggctcgccg ggcccgatat ccacacgatc ttcatgtggg ccgagctgcg gttctacatc
     8521 caggatctcg cgccgtcgcg ggaggaactc gatgcctggc gcgcggtcgc gccaccgcag
     8581 gggtagttcc aacgccggcg ctatcccggt tcaccgccgg ccagcgagtc accccacgcg
     8641 agccggtcgg agccgacgcc ctcggcggtc acgctgcgca gcgcggacag cttctcgcgc
     8701 gcggcgtcag ccgacaggcc gagctggcgg agcagcgcat agccgatcat gccggtgcgg
     8761 tggacaccgg cggagcagtg cacgacaacc ttgccgccac gggcgatcgc ggcggcgatc
     8821 tcggccagcc gcggccgcag ctcggccgtg cgcgcggcgt ccgggacgtc accgccgtcg
     8881 agcggcgtcc acagccaggt gaggcccgcc gcagtcgccg cgttgccgat ctgcttggcg
     8941 ccctcgcgct cggcgagcag cgtgacgaga tgggtcgccc ccagcccgcg tagcgcgggg
     9001 atggccttga gcttggggcg atgggtcagc gcgagcgcgc cgccggcgac cgggacgagc
     9061 acgacatcag agccggagcc actcacgccg ggagtctacc gcgctatgct cggctggcat
     9121 gagcgagccc cgcgtcgagt cggtgagtga agggagcgtc gtcgtcatcc cggatcccga
     9181 tggcgtgctc ggcgcgctca tcgcagcgtt gctcgccgaa cccggcgaga tgcggctgaa
     9241 ggcgatcgca cacggcacgg tggacacgct gtcgctgggc gagctggagt tgcggtgctc
     9301 gaccacgcgc ggcggtgtgg cgccgacctg gagcgtgaac cgcggcagcg cgaccgcgat
     9361 cgtgaccggc gatgtcgtcg agcggctggt tgaccaagct taccagcacc gccgcgcggc
     9421 ggagctggcg acgttcccga tcggcggccg gctcggtcag ggccgctacg tgatcactca
     9481 cgcgctgcgc ggcgggcccg accgtggaat gtatcgcgcg cgcgcggccg gcgaggtgac
     9541 cacctacctc gcgacgcttg ggccgccaca gacccgcgac ttctcggagc tgcggcgcga
     9601 gctcgcgttc gacgtgccgg gcatcgcgcc gctcgagtac atcggccgcc tcgagccgca
     9661 cagcgaggcc ggccacgaag gcatgctcga gcgcgagccc gctgggctac cggctgccga
     9721 gctcgcgcat ccgatggccc gcgacgccac gctccagctc gcgctcggca tcgcccgggt
     9781 gatccgcggc gcgcacgctg ccgggatctt ccttgggacg ctgcgccccg agctggtcta
     9841 cgtgcagtcg gccgcggtgc cggtcgtcac cgggatcgcg ccgcgcgccg aacggttctg
     9901 gctgaccgcc acgccgcgca cgtatggcgt cgcgccatgc ttcgacgact tctaccagtc
     9961 gcccgagcag ctggcgcagc cgcacgatcc agccaccgcc gccagcgacg tgttcgcact
    10021 tggcgcgatg ctcgcgcact ggctcgccgg ggagcacccg ttcgagggcg agggcagcct
    10081 gcaggcgatg tcgatcgcgc tcggtcgccg gcgagccttt gccgggccgc ctgagctcac
    10141 cgcaccgatc gacggcgcgc tcgcgccagc atcggagcgc gtctcgctcg atgcatggat
    10201 ccgcgagctc gagacgctcg cgcgatgacc cgccccgcgt tgactcaccc cggccacacc
    10261 gcgggccacg cagctctcgc ggccccgccc ggatcgtcgg cgacgtggct ctcgcggtcg
    10321 atcagcagcg tggcgcggcg acgggcgtcg tagcgcggcc agctggggag gccgccgcca
    10381 ttgggatcgc cggcgcggat gaactggcac caggcgccgt gcatggcggt ggcgagcggc
    10441 tggagcggag cggcgtcggg gccgagcagg accggcgtca tcggcaggtc gagccggttc
    10501 cacacgaacg gcagctcgag ggcgtgggcg gcgccgagcc ggccgccgaa ggtgggcgac
    10561 ttccagtcga agcggtacat gtagaccggg gcgccgcggg cggactgggc ctcggcgagc
    10621 cggatcgccg ggatgcggaa caccagctcg ccgacgaggt cgacccaggc gcgcttgggc
    10681 gagcgatcgg ggtggccgtc gcggtagagc tcgaccagcg agtcggtgag cgggccgagc
    10741 cggtcgcgca cgagcggctt gaacggctcg acggtggccg cgccgaagaa cacctcgaac
    10801 aggttccact cgtcgcggtt ggtgccgagc aggatcggga tgccggcggc ggcgccggcg
    10861 cggatcgcgt cgatcggcag ccgcggcacg gtgacgccgt cgatgtaggg ggcgtaggcg
    10921 cccaggccgt gggtctgggc gcggcgctcc tgggcggcga gcaggcgatc gatcgggacg
    10981 tcggcgagag cctcgggggt tgcgccgaga tcggcgagca cccagcgcga cacctcggtg
    11041 gcgtcggcgc gggtcggcgg gcccaggctg ggggcgccgc tttcgagaat gacgcggtgg
    11101 aacaggccgc gggcggcggg catcgcgagc agcatgctga tggagatcgc gccggcggac
    11161 tcgcccatga cggtgacctg gccgggatcg ccgccgaacg cggcgatgtg gtccttcacc
    11221 cagcgcagcg cggcgacctg gtcgagcagg aagcagtcgc cggcgtcgtg gccgggggcg
    11281 aggtcgccga ggaacagcag cccgggcagg ccgagccggt agttgacggt gacggtgacg
    11341 atgccctgcc gggcgaacgt ctcgccgtgg tagagcggcg tcgagcccga gcccatgacg
    11401 aacgcgccgc cgtggatcca caccatgacc gggtgcgggc cgggggcggc cggcgcgaac
    11461 acgttgaggt acaggcagtc ctcgccggac tcgatcttgt cggtgatgcc gctcatcatc
    11521 gcgatc
//
LOCUS       DASVOR010000087         4782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_30125,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000087 DASVOR010000000
VERSION     DASVOR010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4782
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30125"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1931)
                     /locus_tag="VF516_01870"
     CDS             complement(<1..1931)
                     /locus_tag="VF516_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX8106438.1"
                     /translation="MKSHVLVYGLLAACHSGSHEACNPACGDGLVCRYATCVVPIPCT
                     ANTDCAGDQYCETAAGECLPWGVGPGGTSDRSCAGTPAPGAFFPAVQCAWSGPPQGDP
                     FPGHVNVLATPMVATLDDPITPSIVFPSYNGTDDHTLASCSGDPKFYGVLRVIDGRTC
                     AQRATLSQVHVVASASVAVGDLGGDDATPEIVAATSAGGLVAYTHRAEGWDLLWQTTS
                     NIAAAQCDWAGPSIHDLDGDGVPEVIFYGAVYDGQTGKAIDESLGTAVDSIGGGYIPV
                     VADVDGDGAPELVTGKHLYSWDKGNRKWTPRKVLPAGDGLVAVGDFGTFPAVGQDDRS
                     HTDGIAEVAVVFSGTLHVFNVAGREVFKASFRVGSGFPQGGPLVIADFDGDGRAEIGS
                     AGFAGYHVFDPDCRPLPGLSTPDPATCATLSTDGVLWTSATQNRQGDLPGSSAFDFDG
                     DGRPEIVSHDQCFTRIYDGTTGKVLSSRPRTSCQWYENPIVADTDGDFNAELITTSNA
                     SCGVSCPAVDPMFDGAQCVDNSDCPVTTLCGRDQAGDALGRCRCAADTDCGDGFVCRD
                     PRAGPSAAGKVCRAAHAAGPMTGVQVLADTADRWIGARPIWNQHAYSITNVGADGVVP
                     RTGQWLLNWTQSGLNSFRV"
     gene            complement(2212..2958)
                     /locus_tag="VF516_01875"
     CDS             complement(2212..2958)
                     /locus_tag="VF516_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106439.1"
                     /translation="MLFSLRELRQIEESRVQEEEHAVRSAEQARIAAAQEAERRRIEA
                     EEAKARAERDELLRIQQARENAEREARLRVESAEAMERQRQQAALEQQRLHQEMELRR
                     AEVAKKRPTWMVAVTIGAVVLTIALGIFAVQRIIQASHDREAAAQAQRDRDEAVRVAQ
                     EAQDRVDKLSHDMKDQDDRLNSADQALRTARTEADRQKAQDELNRLRQQKFEMEKRIQ
                     EARNAAAKAERAKGVHISKECLDNPLAKGC"
     gene            3108..3821
                     /locus_tag="VF516_01880"
     CDS             3108..3821
                     /locus_tag="VF516_01880"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HEX8106440.1"
                     /translation="MGFVDLHSHVLYGVDDGATDEAAALAMLDGLAALGITEQCVTPH
                     QKVGQYLPSQDQIEATLAAVECVRRPGHPTLRLGAESMWDDVFIRRLAEDQLPCYRDT
                     SAFLVEVPPPLLPPGMIDQLFKLRLRSKLAVLAHPERYQPLWGDDELARSLRRVCAFV
                     VDLGAVGGFHGRRETRAARHLLETGLACAVATDLHQIGDLQQAAAGLAWIDKKLGHAA
                     VTRLFDHAPRAILAGELPD"
     gene            3823..>4782
                     /locus_tag="VF516_01885"
     CDS             3823..>4782
                     /locus_tag="VF516_01885"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX8106441.1"
                     /translation="MRLAINLVLSLGMLALCTWLVWPDAAESDQLRQVIGALRWADFA
                     PYLAAWLGLQILVHLCRALRWNYLLAPLGVRVPTGPLLAISSVGFMAILALPVRLGEF
                     VRPGLLRKGGYSTASAALGTVAVERIVDGLIISLVVFCAFFAHRGPSSPGWMMATAYG
                     TLALFTAALVFLAFALRWPVPTVRFCLRLTLLPRYAPRIAETIEHRLLDMIRGFSVLH
                     DVRRLVAFVTWSAVYWGANGLGVWVLAHAFGLPLSLVGAFATMGLLGVGLMLPNSPGL
                     VGQFQWFTLLGMSLYLGFDPRHPGAMALAFAIGQHLLQVVWYVA"
BASE COUNT          606 a         1720 c         1684 g          772 t
ORIGIN      
        1 ttgacgcgga agctgttgag cccggactgg gtccagttga gcagccactg gccggtccgc
       61 ggcaccacgc cgtccgcgcc gacgttggtg atgctgtacg cgtgctggtt ccagatcggg
      121 cgcgcgccga tccagcgatc ggcggtgtcg gcgagcacct ggacgccggt catcggcccg
      181 gcggcgtgcg cggcgcgaca caccttgccg gccgccgacg ggccggcgcg cgggtcgcgg
      241 cagacgaagc cgtcgccgca gtcggtgtcg gccgcgcagc ggcagcgccc gagcgcgtct
      301 ccggcctgat cgcggccgca cagggtcgtg accgggcagt cgctgttgtc gacgcactgc
      361 gcgccatcga acatcggatc gaccgccggg cacgagacgc cgcagctcgc gttcgacgtc
      421 gtgatcagct cggcgttgaa gtcgccatcg gtgtcggcga cgatcgggtt ctcgtaccac
      481 tggcacgagg tgcgcggccg cgaggacagg accttgccgg tcgtgccgtc gtagatccgg
      541 gtgaagcact ggtcatgact gacgatctcg gggcggccgt cgccgtcgaa gtcgaacgcc
      601 gacgagcccg gcaggtcgcc ctggcggttc tgggtcgccg acgtccacag cacgccatcg
      661 gtcgacagcg tcgcgcaggt cgccggatcc ggcgtggaga ggccgggcag cggccggcag
      721 tcgggatcga acacgtgata gccggcgaag ccggccgagc cgatctcggc gcggccgtcg
      781 ccgtcgaagt cggcgatgac cagcggcccg ccctgcggga agccgctgcc gacccggaag
      841 ctcgccttga acacctcgcg gccggcgacg ttgaacacgt gcagtgtgcc actgaagacc
      901 accgcgacct cggcgatccc gtcggtgtgc gatcgatcgt cctggccgac cgccgggaat
      961 gtgccgaagt cgccgaccgc gaccagaccg tcgcccgccg gcagcacctt cctgggcgtc
     1021 cacttccggt tgcccttgtc ccagctgtag aggtgcttac cggtgaccag ttcgggcgcg
     1081 ccgtcgccgt cgacatcggc gaccaccggg atgtagccgc cgccgatcga gtcgaccgcg
     1141 gtgccgagcg actcgtcgat cgccttgccg gtctggccgt cgtacaccgc accgtagaag
     1201 atgacctcgg gaacgccgtc gccgtcgagg tcgtggatcg aggggccggc ccagtcgcac
     1261 tgcgccgcgg cgatgttgga cgtggtctgc cacagcaggt cccagccttc ggcgcggtgg
     1321 gtatacgcga ccagcccgcc cgcgctggtc gcggcgacga tctccggcgt ggcgtcgtcg
     1381 ccgccgaggt ctccgactgc gaccgatgcc gaggcgacca cgtgcacctg cgagagcgtg
     1441 gcgcgctgcg cacaggtccg gccgtcgatc acgcggagca cgccgtagaa cttcgggtcg
     1501 ccggagcacg aggcgagggt gtggtcgtcg gtgccgttgt acgacgggaa cacgatcgac
     1561 ggcgtgatcg gatcgtcgag cgtggcgacc atcggcgtcg ccagcacgtt gacgtggccc
     1621 gggaatggat ctccctgggg cggtcccgac cacgcgcact gcacggccgg aaagaacgcg
     1681 cccggcgccg gcgtgcccgc gcagctccga tcgctggtgc cccccggccc cacgccccac
     1741 ggcaggcact cgccggcggc ggtctcgcag tactggtctc ccgcacagtc ggtgttcgcg
     1801 gtgcacggaa tgggcacgac acaggtcgcg tagcggcaaa cgaggccgtc gccgcaggca
     1861 ggattgcagg cctcgtggct gcccgagtga caggctgcca gaagtccata tacgagaacg
     1921 tgggatttca tcggccggca acctactcgg cgtcctggat ccagggtacc gcgagccctg
     1981 cctgacgtga cggcgtcaac agcgaatgtc tcgcggcgtg tcgtcggcgc gcgaggggcg
     2041 gactcggcgg catgcccggg cgcgcgagca gcgagccgct gttcaccggg cgctcgccgg
     2101 aggaccgcgg cgcacagcca cgagcgcgat cgagggcggg atcgacgtcc ggcgcccgga
     2161 cggacgccgg atgtacgccg gatgtacgcc ggatgtacgc cggatatacg atcaacagcc
     2221 cttggcgagc gggttgtcga gacactcctt cgagatgtgc acgcccttgg cgcgctcggc
     2281 cttggcggcc gcgttccggg cctcctggat ccgcttctcc atctcgaact tctgctggcg
     2341 cagccggttg agctcgtcct gggccttctg gcggtcggcc tcggtccgtg cggtgcgcag
     2401 cgcctggtcg gcggaattga gccggtcgtc ctgatccttc atgtcgtgcg acagcttgtc
     2461 gacccgatcc tgggcctcct gggccacgcg cacggcctcg tccctgtcgc gctgggcctg
     2521 cgccgcggcc tcgcgatcgt gcgacgcctg gatgatgcgc tggacggcga agatgcccag
     2581 ggcgatggtg agcaccacgg cgccgatggt gaccgcgacc atccaggtcg gccgcttctt
     2641 ggcgacctcg gcgcggcgca gctccatctc ctggtgcagg cgctgctgct ccagggcggc
     2701 ctgctggcgc tggcgctcca tcgcctcggc ggactccacc cgcaaccggg cctcgcgctc
     2761 ggcgttctcg cgcgcctgct ggatccggag cagctcgtcg cgctcggccc gggccttggc
     2821 ctcctcggcc tcgatccgcc ggcgctcggc ctcttgcgcc gccgcgatcc gcgcctgctc
     2881 ggcgctgcgc acggcatgct cctcctcctg cacgcggctc tcctcgatct ggcggagctc
     2941 gcgcagcgag aacaacaccg aattctcacg cttctcggac tgtgccatgg accgtccttc
     3001 gtcgcgggcg gctctcccgg gacgtacgat ctctatcacg gcggatctcc tggggcaacg
     3061 agcacaattg tgcccgccgc cctactgcga tacacacccg tctccccatg ggcttcgtcg
     3121 atctgcacag ccacgtgctc tacggcgtgg acgacggcgc gaccgatgag gccgcggcgc
     3181 tcgcgatgct ggacgggctc gcggcgctcg gcatcaccga gcagtgcgtg acgccgcacc
     3241 agaaggtagg ccagtacctg ccgagccagg accagatcga ggcgaccctg gccgcggtcg
     3301 agtgcgtgcg ccggcccggg catccgaccc tgcggctggg cgccgagagc atgtgggacg
     3361 atgtgttcat ccgacggctc gccgaggacc agctgccctg ctaccgcgac acgtccgcct
     3421 tcctggtcga ggtcccacct ccgctgctcc cgcccggcat gatcgatcag ctgttcaagc
     3481 tccgcctgcg aagcaagctc gcggtgctcg cccaccccga gcgctaccag ccgctgtggg
     3541 gcgacgacga gctcgcgcga tcgctgcgcc gggtctgcgc gttcgtggtc gacctcggcg
     3601 cggtgggcgg ctttcacggc cggcgcgaga ccagggcggc gcgccacctg ctcgagaccg
     3661 gcctggcgtg cgccgtggcc acggacctgc accagatcgg cgatctgcag caggcggcgg
     3721 ccggcctggc gtggatcgac aagaagctcg gtcacgccgc cgtgaccagg ttgttcgatc
     3781 acgcgccgcg cgccatcctc gcgggggagc ttccagactg acatgcggct cgcgatcaac
     3841 ctcgtcctgt cgctcggcat gctggcgctg tgcacctggc tggtgtggcc ggacgcggcc
     3901 gagagcgacc agctgcgcca ggtcatcgga gcgctgcgct gggccgactt cgcgccctac
     3961 ctcgccgcct ggctcgggct gcagatcctc gtccacctgt gccgcgcgct gcgctggaac
     4021 tacctgctcg cgccgctcgg cgtccgggtg ccgaccggtc cgttgctcgc gatctcgtcg
     4081 gtcggcttca tggcgatcct cgcgctgccg gtccggctcg gcgagttcgt gcggccgggc
     4141 ctgctgcgca agggtggcta ctcgaccgca tcggcagcgc tcggcaccgt cgcggtcgag
     4201 cgcatcgtcg acgggttgat catctcgctc gtggtgttct gcgcgttctt cgcccaccgc
     4261 ggccccagct cgccgggctg gatgatggcg accgcgtacg gaacgctcgc gctgttcacc
     4321 gccgcgctcg tgttcctggc gttcgcgctg cgctggccgg tgccgaccgt gcggttctgc
     4381 ctccggctca cgctgctgcc gcgctacgcc ccgcggatcg ccgagacgat cgagcaccgg
     4441 ctgctcgaca tgatccgcgg gttctcggtg ctccacgacg tccgccgcct cgtcgcgttc
     4501 gtgacctgga gcgcggtgta ctggggcgcc aacggcctcg gcgtgtgggt gctcgcgcac
     4561 gcgttcggtc tgccgctgtc gctggtcggc gcgttcgcga ccatgggcct gctcggcgtc
     4621 ggcctgatgc tgccgaactc gcccggcctg gtcggtcagt tccagtggtt caccctgctc
     4681 ggcatgtcgc tctacctcgg cttcgacccg cgccaccccg gcgcgatggc gctcgcgttc
     4741 gcgatcggcc agcacctgct gcaggtcgtg tggtacgtcg cg
//
LOCUS       DASVOR010000088        13229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_59275,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000088 DASVOR010000000
VERSION     DASVOR010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13229
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59275"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2855
                     /locus_tag="VF516_01890"
     CDS             <1..2855
                     /locus_tag="VF516_01890"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8106442.1"
                     /translation="SRSADRVLVWSYTGPPAGFAAILDELLAYGRAHHLRVTVLSPVR
                     LEQVGGAAFTATPFGVVQQLEEVAAFTLDGADRQRLRGLVRRFGRAGDVAVAEYVSGS
                     DPAMDRELAELVDRWAAGKPVVNPYTYTIRADLRRGALAGDHRVFVTSVDGVVRAAVI
                     VTRMASQPGYLLDAEFYGDDMPAGGLEHTIVRIIEILRGEGIAIFSFGATFGVKVCDS
                     PNASPDAERALDELRAAGVLGRGNYQFKSKFRPTELPIYLCQPADRPTSVADILVMIG
                     SPEPASRSPIQLAHRAVAIDLLTDSWAERDDPWIAERMRALEPAAAGARDSIEQSWLP
                     FTCVLPTPSGRAAEALLCRSWPGRRGTVLHNGLFPTWVWNLSEQRFTPVAVPGLGEGT
                     ALEDAIARDDQGISFVAVELSCNAAGGQPISLHGLRQIAATARARGLPLVLDATRLVE
                     NALVLAESHGCGVWEMARELLELGDVATFSLSKDWGVNFGGLVASRIPDLDDRLREAA
                     ALRGTDVGLASRRLLQAALGDRDAVLEQVRRRMAAVQALAKPLLEAGLPVVTPVGGHC
                     VLVDVDRIPGFAGRPGEFLTEVFERTGVRGGPHLAGDAHPQLRACCRFAVPVGMTAEA
                     AGRAGALLAGTGAARTEPEDWFSRQLQELGTTLTSAGYAPGNQNLEVLRELVLGIECR
                     MVGVADGDVEVFSAGSGPTLLLMQPFNIGAGIFAPQFAVLSKCFRLIVIHQPGVGRTR
                     VAGSLSLEGIASLQRRVLSAIGVDEPIHVGGASVGAIFAQYFALRFPDRTLSLSLLGG
                     SYRFANRKGQIDRLDQVIAEDFDAIAAGSELPRTAEERDRFTRFLLRCESMDPQTGLR
                     YLDLFTKEPELAPRLGEIAAPTLILHGRHDSVVGVKTGHFLHGAIPDARYVELPRSGH
                     FVCVTDADAVNRELAAFLDEVAVTGRPERTRRAGSR"
     gene            complement(2812..3579)
                     /locus_tag="VF516_01895"
     CDS             complement(2812..3579)
                     /locus_tag="VF516_01895"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8106443.1"
                     /translation="MKNNGIIDRAQIVLQRPDAPPFPRTAAGPMLIGHPEPRFDSRSF
                     YKQLCACMPGRSGSRRLIANEALYTCGTENQNIYFVESGYVKVSLLSQNARQCLLDIC
                     APGDMVGESCLLCTERIETVIAMTQCVVRAIPRTEFLETIFKHKLVEASLQYLTARLL
                     EQQQIVSHFVVADSEGRLAATLLRLARKLGKPHQGRLLIDEKITQEELAEIVGTTRSR
                     VGYFLKRFRATGLIEQPSQAVLLINETRLDEYVQACL"
     gene            complement(3569..4579)
                     /locus_tag="VF516_01900"
     CDS             complement(3569..4579)
                     /locus_tag="VF516_01900"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8106444.1"
                     /translation="MTGKIKLAVITIVALVALTVLHRWQRQDAGPAAGTELAVGYLPV
                     TCHLTCPVTDFATRTTTTSTRFVSQRFSDFPTVVESLKTRKIDATFILAPLAMVLREQ
                     GVPIHIVYLGHRDGSTVIVPKASTARTLRDLKGKTFARPSRYSNQYLVITKLMEDEGM
                     QPEDINFVDMAPPDMPSALAAGAIDAYFVGEPHAAKAELAGTGRVLYYAKDIWPHFIS
                     CVLVVRDELIREHPEIVHDLVRGIAESGEWVERNRLAAAKLAARTEYYNQDQRLLAFV
                     LTQPPDRVSYRQLTPSDEDLRSIMHYALKAKILHKSIALGDLIDRRFIPETIHPAEIH
                     EE"
     gene            complement(4576..5289)
                     /locus_tag="VF516_01905"
     CDS             complement(4576..5289)
                     /locus_tag="VF516_01905"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8106445.1"
                     /translation="MTEMVLEASGVGFDFGDLVVIDRLDLAVAEGEFVACVGPSGCGK
                     TTLLALLGGHLAPARGTLQRRGRTRTIYQDGGLFPWLTVHDNVARGLPDRLDAAIRKR
                     KVAEWLAVTGLTEFAASYPHQLSGGMRQRVELARALAAESDILLLDEPMGALDYQTRL
                     RMRRELCATLRERPRTVVFVTHDIEEAVQLGDRVIVLGPRPTSVRRVIALSPGRPRWP
                     TAPGVPEAEQVIMEELGLT"
     gene            complement(5286..6053)
                     /locus_tag="VF516_01910"
     CDS             complement(5286..6053)
                     /locus_tag="VF516_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8106446.1"
                     /translation="MRPILSPRWYLSLVTGSLLLAAWWLASAARMFPVGTVPSPAEVA
                     SALVSEAHSGRLLENVIASLYRVGWGYLFATITAIPLGLVIGRSASTRDALLPWINFF
                     RNLSPIAWIPFAIIWFGIGDPPAIFLIFLATFFQIVLATAAATASVPRIYYRAAREME
                     FSPVRVLFEVTLPAILPQLVTALRVAAGVAWMVVVAAEMIAVRSGLGFLIVDARNGLR
                     MDLVVCGMLAIGLIGIALDIVFARLARIDSLRWGFER"
     gene            complement(6652..7857)
                     /locus_tag="VF516_01915"
     CDS             complement(6652..7857)
                     /locus_tag="VF516_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4336 domain-containing protein"
                     /protein_id="HEX8106447.1"
                     /translation="MKPFGFCGSNVNAREEQYSAVAHLGVCESESAFSSNSLMSKKGY
                     YWNECSVHFCQSRRCLARSARVSGRPDRHADLRGAGAGWPLTLVRLVPAWSCHRGSTG
                     WAAPGRAVSRSDPEAVQARSCSGVTPGDASRSPAPMSELCYAAPIDRSGQPRLNYRML
                     PVSDDLWQMEGGDLRMPGGALFPIASTVIRLPDRSLVVYSPIELDDAKAAELAALGEV
                     AHLVAPSRFHYLFMRAAAARWPRATVHASPGVAAKCPDLRIDRVLGRDAEPAWRDVLA
                     VEHIGGVPKIDEVVLFHRPSHALVCADLVFHIARPPNLRARIVLALMGAGGGKLAQSR
                     TWGFLRSDRAAAQASVSRILAWPIERVVPCHGAPCAIDASGMASRLTRLAGTRVALPA
                     PTAAPSPSA"
     gene            complement(7854..8060)
                     /locus_tag="VF516_01920"
     CDS             complement(7854..8060)
                     /locus_tag="VF516_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106448.1"
                     /translation="MHSLVIENDVPIALRDGATIYPNVFRPADHARVPVIITLGPCPK
                     DVPFREWNPAAWDRLDVRASDDPR"
     gene            complement(8085..9170)
                     /locus_tag="VF516_01925"
     CDS             complement(8085..9170)
                     /locus_tag="VF516_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8106449.1"
                     /translation="MRRFWTRFSIAIMILGLAHYYVWLRLVSAAQLPPRWHVLATIAV
                     VMLASSLPVAAMALRRLPRATARPYVWLAYTGFGVSVYLLPAAAVTHLACAIAGCDPR
                     TAAILGAGAAAVTVVYGVVHAARGPIVRRVRVPLAKLPASAEGYTIAQLTDVHIGPLL
                     GERFAAKVVAKVNALAPDLIVLTGDLVDGRLEELRRHVEPLRALTARDGVYAVTGNHE
                     YYWNLDVWLEHLRSFGIRILRNEHETIRGAFELAGVDDSSATEDVPRALAGRDAALPV
                     VLLAHHPRTIERAAGAGVDLQLSGHTHGGQLLPLGWLSRLFEPHVAGLARFGATWLYV
                     SEGTGFWGPPMRVGTSSEIALVTLTRG"
     gene            complement(9246..10460)
                     /locus_tag="VF516_01930"
     CDS             complement(9246..10460)
                     /locus_tag="VF516_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8106450.1"
                     /translation="MILVVFLALVAVLVVLALVMGVPFQYNARSLLVRRVTTLATALG
                     IALVVSVFAGALMLGDISRVLAAAGRPDTVIILRKGSDAELSSAIGNDYLGLFRGPAQ
                     VSQSGGVIGEIVVVVIADHIDGTGSSNVLVRGTPAEGIAFRPEIKIVSGRAPRPGTNE
                     IMVGSGIAGRFKGLAIGQSIDLRRNRPLSVVGVFTADGASYESEVWGDLDYVRNALGR
                     QAVVSSARVRLTSAADFDAYRQAIETDKRFSMKVMRESDYNEKQSQATASFLKTMGII
                     IAVLFSIAAMLGAAITMNGAVAHRTREIGTLRALGFTRRSLLTAFLLEAILLAAIGGV
                     IGSVLVLLLSAFVTIPVINFQTFSEIVISFHASPAVFAKSLIFSGAMGLIGGLVPAIR
                     ASRVSPIEAMRG"
     gene            complement(10460..11629)
                     /locus_tag="VF516_01935"
     CDS             complement(10460..11629)
                     /locus_tag="VF516_01935"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8106451.1"
                     /translation="MNAATYVRRNLRRRRGRTILTVLVVMFTVLIFCAIRTFIVSFTS
                     GADQAQADVLAVRHKVSITMQLPKHYIDTLRTVPGVKNATWANWFGAKDPKERLPFFA
                     GFAVDHESYFDVQDAVKVTPEELAAWKQTPNGAILGDQLAKAFSVKVGDRLVVNSDIY
                     PGDWEFKVVGIYTPVRTIADRTSLIFRWDYLNNDSRAVFSKEKIGWMIARVTDPAGGA
                     EIAKRIDAKFDGNDDQTLTMSERAFALSFLGGFAAVLRAFNVVSLVILLIMALILANT
                     IAMSVRERTHEYGVLRAIGFPPARILGFIVGESLLIAVVGGVLGIAVTSLVINHAVGP
                     LMAANMSGAFANFFTPGWVFGVALAAAAALGLVAGLLPALLVPPRKITDALRRVD"
     gene            complement(11626..12381)
                     /locus_tag="VF516_01940"
     CDS             complement(11626..12381)
                     /locus_tag="VF516_01940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8106452.1"
                     /translation="MSETTTGSETPDGTANGTASEPYRGTVTTPTIVKLSGIAKTFYR
                     GSEPIHVLNGLDLDVPNGSFEALMGPSGSGKSTLLNIIAGLDRPSSGTVLVAGADLTR
                     MSDSQLARWRSQTIGFVFQSFNLIPVLTAAENVELPLLLTSLSRAERAKHVATALRIV
                     GLEDRMHHYPRQLSGGQEQRVAIARAIVNDPKIIVADEPTGDLDRKSADDVLTLMERL
                     NKDLHKTIFMVTHDAAAAERAGVLRKLDKGALQ"
     gene            complement(12381..>13229)
                     /locus_tag="VF516_01945"
     CDS             complement(12381..>13229)
                     /locus_tag="VF516_01945"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8106453.1"
                     /translation="LTEIKVQIEREKPMVEKGVTAKATLDDLEARRGSAEAAVHASEA
                     EAMAGDADVKALEVQLGSYEIITPISGTVVDKLVKVGEGVSPGFGTPGVIEVIDLTSL
                     VVEVDVPEGRLSQVAVDAPCEIVLDAYPTRRYRGAVKEIGRRVNRSKATVPVKIRFVD
                     LADGRPVPGGAPDPGSPSEPGARKPEILPDMAARVSFLASAIDPATLDTPPKLTVPAG
                     AVVQRNGRDVVFVVDEGEVRMTPVALGAASGDGRELVTKLPPGTKVVLDPPADLGDGE
                     KVKENK"
BASE COUNT         2111 a         4562 c         4594 g         1962 t
ORIGIN      
        1 tctcgcgcag cgccgaccgc gtgctggtgt ggagctacac cgggccgccc gccgggttcg
       61 cggcgatcct cgacgagctc ctcgcgtacg gccgcgcgca tcacctgcgc gtcaccgtgc
      121 tgtcgccggt gcggctcgag caggtcggcg gcgcggcgtt caccgcgacc ccgttcgggg
      181 tcgtccagca gctcgaggag gtcgcggcgt tcacgctcga cggagcggac cggcagcgcc
      241 tgcgcggcct ggtgcggcgg ttcggacgcg ccggcgacgt cgccgtcgcc gagtacgtct
      301 cgggcagcga ccccgccatg gatcgggagc tcgccgagct cgtcgatcgc tgggcggccg
      361 gcaagccggt cgtcaacccc tacacctaca cgatccgcgc cgacctgcgc cgcggtgcgc
      421 tcgccggcga tcatcgcgtg ttcgtgacct cggtcgacgg cgtggtgcgc gccgccgtga
      481 tcgtcacgcg gatggcctcg cagcccggct acctcctcga cgccgagttc tacggcgacg
      541 acatgccggc aggcggtctc gagcacacca tcgtccggat catcgagatc ctgcgcggcg
      601 aggggatcgc gatcttcagc ttcggcgcga cgttcggcgt gaaggtctgc gattccccca
      661 acgcctcgcc ggacgcggag cgcgcgctcg acgagctgcg cgcggcgggc gtgctcggcc
      721 ggggcaacta ccagttcaag agcaagttcc ggcccaccga gctgccgatc tacctgtgcc
      781 agccggccga tcgaccgacg agcgtggccg acatcctggt gatgatcggg agtccggagc
      841 cggcgtcgcg cagcccgatc cagctcgcgc accgcgcggt cgcgatcgat ctgctgaccg
      901 actcgtgggc cgagcgcgac gatccgtgga tcgccgagcg gatgcgcgcg ctggaaccgg
      961 cggcggccgg cgccagggac tcgatcgagc agtcgtggct gccgttcacc tgcgtgctcc
     1021 ccacgcccag cggccgcgcc gcggaggcgc tgctgtgccg cagctggccc gggcggcgcg
     1081 gcaccgtcct gcacaatggc ctgttcccga cctgggtatg gaacctctcc gaacagcggt
     1141 tcacgccggt cgcggtgccc ggtctcggcg aggggaccgc gctcgaggac gcgatcgcgc
     1201 gggacgacca gggcatctcg ttcgtcgccg tcgagctgtc gtgcaatgcc gcgggcggcc
     1261 agccgatctc gctgcacggc ctgcgacaga tcgccgcgac cgcgcgcgcg cggggcctac
     1321 cgctcgtcct cgacgcgacc cgtctggtcg agaacgcgct ggtcctcgcc gagagccatg
     1381 gctgcggcgt gtgggagatg gcgcgggagc tgctcgagct cggcgatgtc gccacgttca
     1441 gcctgtcgaa ggactggggc gtgaacttcg gcggactggt ggcgagccgg atcccggatc
     1501 tcgacgaccg gctgcgcgag gcagccgcgc tgcgcggcac cgacgtcggc ctggcaagcc
     1561 gccggctgct ccaggccgcg ctgggcgatc gggacgccgt gctcgaacag gtccgccggc
     1621 gcatggcggc cgtccaggcc ctggcgaagc cgctcctcga ggccgggctg ccggtggtga
     1681 ccccggtcgg cggtcactgc gtgctcgtcg acgtcgaccg catccccggc tttgcggggc
     1741 gtccgggcga gttcctgacc gaggtgttcg aacgcaccgg cgtgcgcggt gggccgcatc
     1801 tcgccggcga tgcccatccg cagctgcggg cgtgctgtcg ctttgccgtc cctgtgggta
     1861 tgaccgccga ggcggccgga cgcgccggcg cgctgctggc cggcacgggc gccgcgcgca
     1921 ccgaacccga ggactggttc tcgcgccagc tccaagagct cggcacgacg ctgaccagcg
     1981 ccggctatgc gccgggcaac cagaacctcg aggtgctccg cgagctggtc ctggggatcg
     2041 agtgccggat ggtcggcgtc gcggacggcg acgtcgaggt gttcagtgca gggagcggcc
     2101 cgacgctgct gctgatgcag ccgttcaaca tcggcgcggg gatcttcgcg ccgcaattcg
     2161 ccgtgctgtc gaagtgcttc cggctgatcg tgatccacca gcccggtgtc ggccggacgc
     2221 gggtggccgg aagcctgagc ctcgagggga tcgcgagcct gcagcggcgc gtgctctcgg
     2281 cgatcggcgt cgacgagccg atccatgtcg gcggcgcgtc ggtcggggcg atcttcgccc
     2341 agtacttcgc gctgcggttt cccgaccgga cgctgtcgct cagcctgctc ggcggctcgt
     2401 atcggttcgc caaccgcaag ggccagatcg accgcctcga ccaggtgatc gccgaggact
     2461 tcgacgcgat cgcggcgggg agcgagctgc cccgcaccgc cgaggagcgt gaccggttca
     2521 cccggttcct gctgcgctgc gagagcatgg atccgcagac cggcctgcgc tacctcgacc
     2581 tgttcacgaa ggagcccgag ctcgcaccgc ggctcggcga gatcgcggcg ccgaccctga
     2641 tcctgcacgg gcggcatgac tccgtggtgg gcgtcaagac cgggcacttc ctgcatggcg
     2701 cgatcccgga cgcgcgctac gtcgagctac cccggtccgg tcacttcgtc tgcgtcaccg
     2761 atgccgatgc ggtcaaccgg gagctcgcgg cgttcctcga cgaggtcgcg gtcacaggca
     2821 ggcctgaacg tactcgtcga gccgggtctc gttgatcaac agcacggcct gggacggctg
     2881 ctcgatcagg ccggtggccc ggaaccgctt gaggaaatag ccgacccggg accgcgtggt
     2941 cccgacgatc tcggcgagct cctcctgcgt gatcttctcg tcgatcagca gccgcccctg
     3001 gtgcggcttc ccgagcttgc gcgcgagccg cagcagcgtc gccgccagcc ggccttcgct
     3061 gtccgcgacg acgaagtgcg agacgatctg ctgctgctcg agcaaccgcg cggtgaggta
     3121 ctgcaggctg gcctcgacga gcttgtgctt gaagatggtc tcgaggaact cggtgcgcgg
     3181 gatcgcccgc acgacacact gagtcatcgc gatgacggtc tcgatccgct cggtacacag
     3241 caggcacgac tcgccgacca tgtctcccgg tgcgcagatg tcgagcaagc attgccgcgc
     3301 gttctgcgac aggagcgaaa ccttcacata gccgctctcg acaaaataga tgttctggtt
     3361 ctcggtgcca cacgtgtaga gcgcctcgtt cgcgatcagc cgcctcgacc ccgaccgacc
     3421 cggcatgcag gcacagagct gcttgtaaaa cgagcgcgag tcgaaccgcg gctcggggtg
     3481 gccgatcagc atcggaccgg ccgcggtccg gggaaacggc ggagcgtccg gacgttgcaa
     3541 cacgatctgc gcccggtcga tgattccgtt attcttcatg gatctcggcg ggatgaatgg
     3601 tttccggaat gaaccgacgg tcgatgaggt cgccgagggc gatgctcttg tgcaagatct
     3661 tggccttcag cgcgtagtgc atgatcgaac gcagatcctc gtcgctcgga gtcagctgcc
     3721 ggtacgagac ccggtcgggc ggctgggtca gcacgaacgc caggagcctc tgatcttgat
     3781 tgtaatattc ggtcctggcg gccagcttcg ccgcggcgag ccggttgcgt tccacccact
     3841 cgccgctctc ggcgatcccc cgcacgaggt cgtggacgat ctccgggtgc tcgcggatca
     3901 gctcgtcacg aacgacgagc acgcacgaga tgaagtgcgg ccagatgtcc tttgcgtagt
     3961 acaggacccg accggtgccg gcgagctcgg ccttcgcggc atggggctct ccgacgaagt
     4021 atgcatcgat cgcgccagcc gcgagcgcgc tcggcatgtc gggcggcgcc atgtcgacga
     4081 agttgatgtc ctcgggctgc atgccctcgt cctccatcag cttggtgatc acgaggtact
     4141 gattcgaata cctgctgggg cgcgcgaacg tcttgccctt gagatcgcgc agcgtcctgg
     4201 cggtcgacgc tttcggcacg atgaccgtcg aaccgtcgcg gtgaccgaga tagacgatgt
     4261 ggatcgggac gccctgctcg cgcagcacca tcgcgagcgg cgccaggatg aacgtcgcat
     4321 cgatcttccg ggtcttgagc gactcgacca ccgtcgggaa gtcggagaac cgctgggaca
     4381 cgaaccgtgt cgaggtggtg gtcgtcctgg tcgcgaaatc ggtcaccgga catgtcaggt
     4441 gacaggtgac cggcagatag ccgaccgcga gctcggtccc tgccgccggc ccggcatcct
     4501 gccgctgcca tcgatgcagg accgtgagcg cgaccagagc gacgatcgtg atcaccgcaa
     4561 gtttgatttt gccggtcatg tcaatccgag ctcctccatg atgacctgtt cggcctccgg
     4621 aactcccggc gcggttggcc accgcggacg gcccggggac agcgcgatca cgcggcgaac
     4681 gctggtcggc cggggcccca ggacgatcac gcgatcgccg agctggacgg cttcctcgat
     4741 gtcgtgcgtc acgaacacca cggtgcgcgg ccgctcgcgc agcgtcgcgc acagctcgcg
     4801 tcgcatgcgc aaccgggtct ggtagtcgag cgctcccatc ggttcgtcga gcagcaggat
     4861 gtcggactcc gccgccagcg cgcgagccag ctcgacccgt tgccgcatgc cccccgacag
     4921 ctgatgcggg tagctcgctg cgaactcggt gaggcccgtc accgcgagcc actcggccac
     4981 cttgcgcttc cggatcgcgg catcgagccg gtccggcagg ccgcgcgcca cgttgtcgtg
     5041 aaccgtcagc cacggaaaca gcccgccatc ctggtagatc gtgcgggtgc ggccgcggcg
     5101 ctgcagcgtg ccgcgcgcgg gtgcgaggtg cccgcccagc agcgcgagca aggtggtctt
     5161 gccgcaaccc gacggtccga cgcaggcgac gaactcgccc tcggcgaccg cgagatcgag
     5221 ccggtcgatc acgacgagat cgccaaagtc aaacccgaca ccgcttgctt cgagcaccat
     5281 ctcggtcatc gctcgaaccc ccagcgcagg ctatcgatcc tggcgagccg cgcgaacacg
     5341 atgtccagcg cgatcccgat caacccgatc gcgagcatcc cacacaccac gaggtccatg
     5401 cgaagcccgt tgcgcgcgtc gacgatcagg aagccaagcc cgctgcgcac ggcgatcatc
     5461 tcggcggcaa cgacgaccat ccaggccacc ccggcggcga cccgcagggc ggtcacgagc
     5521 tgtggaagga tcgcagggag cgtgacctcg aacagcaccc ggaccgggct gaactccatc
     5581 tcgcgcgccg cgcggtagta gatgcgcggc accgaggcgg tcgccgctgc agtcgccagc
     5641 acgatctgga agaacgtcgc caggaagatc aagaagatcg ccggcggatc gccgatcccg
     5701 aaccagatga tcgcgaacgg gatccatgcg atcggcgaca ggttccggaa gaagttgatc
     5761 cacggcagca gcgcgtcgcg cgtcgacgcc gaccggccga tcaccaggcc gagcgggatc
     5821 gcggtgatcg tcgcgaacag gtagccccag ccaacccggt acagcgacgc gatgacgttc
     5881 tcgagcaacc gtccggagtg cgcctccgat acaagagcgc tggcgacctc cgcaggcgac
     5941 gggaccgtgc cgaccggaaa catccgggcc gccgatgcca gccaccatgc tgcgagcagc
     6001 agtgagccgg tcacgagcga caggtaccag cgcggcgaca ggatcggccg catacgtggt
     6061 attccgcgga ccggagacgc tatcgttgcc tgcatggcat gctcgaaatc ggcgaagacg
     6121 accgcctcgc ggcagacacg gaagcgccat gcgagcagcg caatcggaac ctccgtacgg
     6181 aataggccgt gattttcaat gcggccgaaa ttgctccccg ggcgggagaa agcgccacag
     6241 caaattgacg cgaggcatcc tcggaaccct cagctgccac cgtgcgattc cgtgacatgc
     6301 tcatcgaacc tcctcggagt gcgcctttgc gcggccagtg tttgccccgt cgttctgata
     6361 gtgcgcaaac gccggggccc gctgctcaat tgtgagcaac aagcagcgtc gtacacgata
     6421 tctcgaactc tgtgagcacc gctttgtggt actcccatcg cagcgcgagc ggtggcgcac
     6481 gcccgtgaca atggattctg tcgaatcggg tcgggccggc ttggaatcac gaggcgcgcg
     6541 tgaacgctcg gtgacgcgcg cacccctctt cgatcgaggg ggagcgcaag gtttgcgcct
     6601 cgatgcaggg cgcgcgcatt cgcccgggcc gggcagcgat gcgacggagc cctatgcgct
     6661 gggcgacggc gccgcggtcg gcgccggcaa cgcgacgcgc gtgcctgcga ggcgggtcag
     6721 tcgcgacgcc atccccgacg catcgatcgc gcatggcgca ccgtggcagg ggacgacgcg
     6781 ttcgatcggc cacgcaagga tccgggacac cgatgcctgg gcagcggcgc gatcggaccg
     6841 caagaacccc caggtccgac tctgggcaag cttgcctccc cccgcgccca tgagcgccag
     6901 cacgatgcgc gcgcgcaggt tcggcggccg ggcgatgtga aacaccagat cggcacatac
     6961 cagcgcgtgg ctgggccgat gaaacaggac gacctcgtcg atcttgggca cgccgccgat
     7021 atgctcgacc gcgagcacgt cgcgccacgc cggctccgcg tcgcgtccga gcacgcgatc
     7081 gatccgcaga tccgggcact tggccgcaac gccgggggag gcgtgcacgg tcgcgcgcgg
     7141 ccagcgagcc gctgcggccc gcatgaacag gtagtggaac cggctcggcg cgaccagatg
     7201 agcgacctcc ccgagcgccg ccagctccgc cgccttcgcg tcgtcgagct cgatcggcga
     7261 gtacacgacc agcgagcgat cgggcaggcg gatcaccgtg gatgcgatcg ggaacaacgc
     7321 gccccccggc attcgcagat cgcccccctc catctgccag agatcgtcgc tgacggggag
     7381 catgcggtag ttcaagcgcg gctggcccga cctgtcaatc ggcgcagcgt aacaaagctc
     7441 actcatcggg gcggggctcc gtgaggcgtc gccgggggtg actccggagc acgatcgggc
     7501 ctgcaccgct tccgggtcgc tgcgcgaaac cgctcgtcct ggggccgccc agccagtcga
     7561 cccgcggtgg cacgaccacg cgggaacgag ccggaccagg gtcagcggcc agccagcacc
     7621 ggcacctcgt agatccgcat gccgatcggg ccgaccagag accctggcgg aacgggcgag
     7681 gcagcgacga gactgacaaa agtgcacaga gcactcgttc cagtaatagc ctttcttgct
     7741 catcaaggaa ttcgaggaaa aggcggattc ggattcgcaa actccaaggt gcgcgacagc
     7801 gctgtattgt tcttcccgag cattgacatt cgatccgcag aagccgaacg gcttcacctc
     7861 gggtcatcgc tcgcgcggac gtcgaggcga tcccacgcag cggggttcca ctcgcgaaac
     7921 ggcacgtcct tgggacaggg accgagcgtg atgatgaccg gcacgcgcgc atggtcggca
     7981 ggccgaaaca cgttggggta gatcgtggcg ccgtcgcgca gcgcgatggg tacgtcgttc
     8041 tcgatgacga gtgagtgcat ggtgctctcc ggttcgagcc tcgatcagcc cctggtgagc
     8101 gtcacgagcg cgatctccga cgaggtcccg acgcgcatcg gcggccccca gaagccggtg
     8161 ccttcgctga cgtagagcca cgtcgcgccg aaccgtgcga ggccggcgac gtgtggctcg
     8221 aacaagcgcg acagccagcc gagcggcagg agctggccac cgtgggtgtg acccgagagc
     8281 tggagatcga cgccagcgcc ggccgcgcgt tcgatcgtgc gcgggtgatg cgcgagcagc
     8341 acgacgggca gggccgcgtc gcgcccggcg agcgcgcgcg gaacgtcctc ggtggcgctc
     8401 gaatcgtcga cgcccgcgag ctcgaatgcg ccgcggatcg tctcgtgctc gttgcgcagg
     8461 atgcggatgc cgaacgagcg caggtgctcg agccagacat cgaggttcca gtagtactcg
     8521 tggttgccgg tgaccgcgta cacaccatcg cgcgccgtca acgcgcgcag cggctcgacg
     8581 tggcggcgca gctcctcgag ccggccatcg acgaggtcgc cggtgaggac gatgagatcc
     8641 ggtgccagcg cgttgacctt cgcgaccacc ttggcggcga atcgctcgcc gagcagcgga
     8701 ccgatgtgaa cgtcggtgag ctgggcgatc gtgtagccct cggcgctcgc tggcagcttc
     8761 gcgagcggga cgcgcacgcg ccggacgatc ggcccgcggg cggcgtgcac gacgccatag
     8821 accacggtca ccgcggcggc cccggccccg aggatggcgg cggtgcgcgg atcgcagccg
     8881 gcgatcgcgc acgcgaggtg ggtcaccgcc gcggccggga gcaggtacac cgagacaccg
     8941 aacccggtgt acgcgagcca gacgtacggc ctggcggtcg cgcgcggcag ccgccgcagc
     9001 gccatcgccg cgaccggcag gctcgacgcg agcatgacga ccgcgatcgt ggccagcacg
     9061 tgccaccgcg gcggcagctg cgccgcggag accaggcgca gccacacgta gtagtgcgcc
     9121 aggccgagga tcatgatcgc gatgctgaac cgggtccaga accgacgcac ggacggtgta
     9181 tagcatcgac cggctctgat ccgggagaag ccatcgccgc ccgcgatggc tcacccgatc
     9241 gccggtcagc cgcgcatcgc ctcgatcggc gacacccggg acgcgcggat cgccgggacc
     9301 agcccgccga tcaggcccat cgcgcccgag aagatcagcg acttggcgaa caccgccggt
     9361 gaggcgtgga agctgatcac gatctcggag aaggtctgga agttgatcac cgggatggtg
     9421 acgaacgcgg acagcaggag caccagcacg ctgccgatca cgccgccgat cgccgcgagc
     9481 aggatcgcct cgaggaggaa cgcggtcagg agcgaccgcc gcgtgaaccc gagcgcgcgc
     9541 agcgtgccga tctcgcgggt gcggtgggcg accgcgccgt tcatcgtgat cgcggcgccg
     9601 agcatcgccg cgatcgagaa caggaccgcg atgatgatcc ccatcgtctt gagaaagctc
     9661 gcggtcgcct gcgactgctt ctcgttgtag tccgattcgc gcatcacctt catgctgaat
     9721 cgcttgtcgg tctcgatcgc ctggcggtag gcgtcgaagt cggccgcgct ggtgagccgg
     9781 acccgcgccg acgacaccac ggcctggcgg ccgagcgcgt tgcgcacgta gtcgagatcg
     9841 ccccagacct cggactcgta cgacgcgccg tcggcggtga acacgccgac gaccgacagc
     9901 ggccggttgc ggcgcagatc gatgctctgt ccgatcgcga ggcccttgaa ccgcccggcg
     9961 atgccggagc cgaccatgat ctcgttggtc cccggcctgg gcgcccggcc gctgacgatc
    10021 ttgatctcgg ggcggaacgc gatgccctcg gcgggggtgc cgcggaccag cacgttggac
    10081 gagcccgtgc cgtcgatgtg atcggcgatc acgacgacga cgatctcgcc gatcacgccg
    10141 ccggactgcg atacctgcgc cgggccgcgg aacagcccga ggtagtcgtt gccgatcgcg
    10201 ctcgacagct cggcgtcgga gcccttgcgc aggatgatca cggtgtcggg ccggcccgcc
    10261 gcggcgagca cgcggctgat gtcgccgagc atcagcgcac cggcgaacac ggacacgacg
    10321 agcgcgatgc ccagcgcggt cgccagcgtg gtgaccctgc ggaccagcag gctgcgtgcg
    10381 ttgtactgga atggcacgcc catgaccagc gcgagcacga ccaggacggc cacgagggcc
    10441 agaaagacca cgaggatcat cagtccaccc tcctcagcgc atcggtgatc ttgcgcggcg
    10501 gtacgagcaa cgcgggcagc agcccggcga ccaggccgag cgccgcggcg gcggcgagcg
    10561 cgacgccgaa cacccagccg ggcgtgaaga agttcgcaaa cgcgccggac atgttcgcgg
    10621 ccatcagcgg accgaccgcg tggttgatca ccagcgacgt gacggcgatc ccgagcacgc
    10681 cgccgaccac ggcgatcagc agcgactcgc cgacgatgaa gccgaggatg cgcgccggcg
    10741 gaaagccgat cgcgcgcagc accccgtact cgtgggtccg ctcgcgcacg ctcatcgcga
    10801 tcgtgttggc caggatcagc gccatgatca gcaggatcac gagcgagacc acgttgaacg
    10861 cgcggagcac cgcggcgaac ccgccgagga acgacagcgc gaacgcgcgc tcgctcatgg
    10921 tcagcgtctg gtcgtcgttg ccgtcgaact tggcgtcgat ccgcttggcg atctccgcgc
    10981 cgcccgcggg atcggtgacc cgggcgatca tccagccgat cttctccttg gagaacaccg
    11041 cccgcgaatc gttgttgagg tagtcccagc ggaagatcag gctggtgcga tcggcgatcg
    11101 tgcggaccgg cgtgtagatg ccgacgacct tgaactccca gtcacccgga tagatgtcgc
    11161 tgttgaccac caggcgatcg ccgaccttga cgctgaacgc cttggcgagc tggtcgccga
    11221 ggatcgcgcc gttcggggtc tgcttccacg cggcgagctc ctcgggcgtg accttcacgg
    11281 cgtcctggac gtcgaagtag ctctcgtggt cgaccgcgaa gccggcgaag aacggcaggc
    11341 gctccttggg gtccttggcg ccgaaccagt tcgcccaggt cgcgttcttg acgcccggga
    11401 ccgtgcgcag cgtgtcgatg tagtgcttgg gcagctgcat cgtgatcgac accttgtggc
    11461 ggacggcgag cacgtcggcc tgcgcctggt cggcgcccga ggtgaacgac acgatgaacg
    11521 tgcggatcgc gcagaagatc agcacggtga acatgaccac gaggacggtc aggatcgtgc
    11581 ggccgcgccg gcggcgcagg ttgcgccgga cataggtcgc cgcgttcatt gcagcgcccc
    11641 cttgtcgagc ttgcgcagca cgccggcgcg ctcggccgcg gcggcgtcgt gggtcaccat
    11701 gaagatcgtc ttgtgcaggt ccttgttgag ccgctccatc agggtcagca cgtcgtccgc
    11761 gctcttgcgg tcgaggtcgc cggtcggctc gtcggcgacg atgatcttcg gatcgttgac
    11821 gatcgcgcgc gcgatcgcga cccgctgctc ctggccgccc gacagctggc gcgggtagtg
    11881 gtgcatccgg tcctcgagcc cgacgatccg cagcgcggtc gcgacgtgct tggcccgctc
    11941 ggcgcgcgac agcgaggtca agagcagcgg cagctcgacg ttctccgccg cggtgagcac
    12001 cgggatcagg ttgaacgact ggaacacgaa gccgatggtc tgcgaccgcc acctggcgag
    12061 ctggctgtcc gacatccggg tgaggtcggc gccggcgacc agcacggtgc cgctgctcgg
    12121 ccggtcgagg ccggcgatga tgttgagcag cgtcgacttg cccgagcccg acggccccat
    12181 cagcgcctcg aacgagccgt tgggcacgtc gaggtcgagg ccgttcagca cgtggatcgg
    12241 ctcgctgccg cgatagaacg tcttggcgat cccggacagc ttgacgatcg tcggcgtggt
    12301 cacggtgccg cggtacggct cgctggctgt gccgttcgcg gtcccgtccg gagtctcgct
    12361 gcctgtcgtg gtctcgctca tcacttgttc tccttgacct tctcgccgtc gccgagatcg
    12421 gccggcggat cgagcacgac cttggtgccc ggcgggagct tggtgaccag ctcgcggccg
    12481 tcgccgctgg ccgcgccaag cgcgaccggg gtcatccgga cctcgccctc gtcgacgacg
    12541 aacaccacgt cgcggccgtt gcgctggacg accgcgcccg ccggcacggt cagcttgggc
    12601 ggcgtgtcga gcgtcgccgg gtcgatggcc gacgccagga agctgacccg cgccgccatg
    12661 tcggggagga tctccggctt gcgggcgccc ggctcgctcg gcgaaccggg atcaggagcc
    12721 cccccgggaa ccggcctgcc gtccgccagg tcgacgaacc ggatcttgac cggcacggtg
    12781 gccttcgagc ggttgacccg ccggccgatc tccttgaccg cgccgcggta ccgccgggtc
    12841 ggataggcgt cgagcacgat ctcgcacggc gcatcgaccg cgacctgcga cagccggccc
    12901 tcgggcacgt cgacctcgac gaccagcgac gtcaggtcga tgacctcgat cacgccgggc
    12961 gtgccgaagc cgggcgacac gccctcgccg accttcacca gcttgtcgac caccgtcccc
    13021 gagatcggcg tgatgatctc gtagctgccg agctggacct cgagcgcctt gacgtcggcg
    13081 tcgccggcca tcgcctcggc ctcggaggca tgcaccgcgg cctcggccga gccccggcgg
    13141 gcctccagat cgtccagcgt ggccttggcg gtgacgccct tctccaccat cggcttctcg
    13201 cgctcgatct ggaccttgat ctcggtcag
//
LOCUS       DASVOR010000089         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_468505,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000089 DASVOR010000000
VERSION     DASVOR010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468505"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1680)
                     /locus_tag="VF516_01950"
     CDS             complement(<1..1680)
                     /locus_tag="VF516_01950"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF009586.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8106454.1"
                     /translation="MWTLASIVIAIVVAIALLCAGWPWRAWVIGWVPLLARWAAAGGG
                     WGLAIAMAVWLGVAIATGVPAIRRRALSAPMMRWLSARGPRIGDTERIALEAGTVWWD
                     AELFSGAPRWERIVRFVPRPLSERERMFLDGPCTDLCRLLDDHAIRAAGDLPAEVWHF
                     LKRERFLGMIIPEEFGGLGFSAIAHSAVVARLASRSAAACVTVMVPNSLGPAELLLRY
                     GTPEQKRFYLPRLAAGDEIPCFALTEPRAGSDAAAMRATGVVCEGVYDGRRVVGMKLT
                     WDKRYVTLAPVATVLGLAFKLVDPGRLLGGDVELGITCALIPVDTRGVSIGLRHDPMG
                     MAFHNGPTQGTDVFVPLEQIIGGPAMIGQGWRMVMESLSAGRSLSLPADAVGGAQLAT
                     RAISAYASVREQFGVPIGRFEGVQAGLGRIAGTLYWMNAVRTVTAGAVDASERPSVIS
                     AIAKRWSTEALRRVASDAMDIAGGFGICRGPRNPLAPIYESVPIGITVEGANILTRSL
                     IVFGQGAIRCHPYVLTELRAAEARDVVAFDRTLVRHAGFTLRNAARSFVLAL"
     gene            complement(1746..2327)
                     /locus_tag="VF516_01955"
     CDS             complement(1746..2327)
                     /locus_tag="VF516_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106455.1"
                     /translation="MSSCALTVAALAVALPACGDVAAKRDVACGAQPVQVLPNGSFDA
                     ATPAWMQDPVTTALICGPPKITPVDGTQAGCLGGTDGTVQTLSQQVPLPDGAKSVTLS
                     GQICIDTKETQAADHDVLQLDLLDGATVIAALGKMTNQQGATPCVFKALTLTAALTGD
                     PVTATLRIRSTLDTSMPTSFYLDALSLTVSCMQ"
     gene            2729..3715
                     /locus_tag="VF516_01960"
     CDS             2729..3715
                     /locus_tag="VF516_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106456.1"
                     /translation="MNGLGILCTARDALTLDELGRVAGWTSEPQRRTFLGRARELLIE
                     TRRTSRVFEYRLHHDSIRAYIADAVGSDALAAHHLALAQRLATWPAPADAGARRYALR
                     HALLHRAEAGAWADAWRVAADMAFLEVKCRALGAHDAEADVERTATRCRASGDDAHGD
                     RLTDLARALGRESHWLRAAPKATAALVWNGLHRLGWCASDLDAQLRVPEGREFLRVRH
                     AATRESPALVRSLEGHTHAVMACAPTPDGRHAVSASSDHTLKVWELASGRPVATLQGH
                     TDRVTACAVTPDGRHVVSASDDHTLKVWDLGTYTAARPRCTPTSAIPTAGST"
     gene            3673..4506
                     /locus_tag="VF516_01965"
     CDS             3673..4506
                     /locus_tag="VF516_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8106457.1"
                     /translation="MYADLGDPDRGLDLIERHAISFLYAIPSYVQALIDAQRRRPRRI
                     DALRTVVTGSAAVPPHLIGLARDVLGVRVQTLWGMTEIGGATFTWPDDPADWPAHSDG
                     RPIEWMEIDIADEADGVGRLRVRGANQCLGYHQRPDVYAAAIAGDGWFDTGDLVRRDG
                     RGGIRIVGRVKDVIVRNGHKVPVVEVESALAAHPQVAMVALVPDPDAQLGERICAVVV
                     PRAPAPTLTELRDHLRALGMSAQYWPDRLHLVREMPLTANGKIQNYVLAELLRAPGPV
                     P"
     gene            4503..>4663
                     /locus_tag="VF516_01970"
     CDS             4503..>4663
                     /locus_tag="VF516_01970"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8106458.1"
                     /translation="MNFAPGDDQRLIQRTAREVADRLLAPRAAARDASGEFPVAELAE
                     LARLGLLGIT"
BASE COUNT          681 a         1696 c         1709 g          577 t
ORIGIN      
        1 gagcgcgagc acgaagctgc gcgccgcgtt gcgcagcgtg aacccggcgt ggcgcaccag
       61 ggtgcgatcg aacgcgacga cgtcgcgcgc ctccgccgcg cggagctcgg tgagcacgta
      121 cgggtggcag cggatcgcgc cctggccgaa cacgatcagc gaccgggtca ggatgttggc
      181 gccctcgacc gtgatcccga tcggcacgct ctcgtagatc ggcgcgagcg ggttgcgcgg
      241 gccgcggcag atcccgaagc cgccggcgat gtccatcgcg tcgctggcga cccggcgcag
      301 cgcctcggtc gaccaccgct tcgcgatcgc cgagatcacg ctcggtcgct cgctggcgtc
      361 gaccgcgccg gcggtcaccg tgcggacggc gttcatccag tacagcgtgc cggcgatccg
      421 gccgagcccg gcctgcacgc cctcgaaccg gccgatcggc acgccgaact gctcgcgcac
      481 cgacgcgtac gcggagatcg cgcgggtcgc gagctgcgcg ccgccgaccg cgtccgccgg
      541 caggctcagc gaccgcccgg ccgacagcga ctccatcacc atgcgccacc cctgcccgat
      601 catcgccgga ccgccgatga tctgctcgag cgggacgaac acgtcggtgc cctgggtcgg
      661 gccgttgtga aacgccatcc ccatcggatc gtggcgcagc ccgatcgaca cgccgcgggt
      721 gtcgaccggg atcagcgcgc aggtgatgcc gagctcgacg tcgccgccga gcaggcggcc
      781 cggatcgacc agcttgaacg cgagcccgag gacggtcgcg accggcgcga gcgtgacgta
      841 gcgcttgtcc caggtcagct tcatgccgac cacgcggcgg ccgtcgtaca cgccctcgca
      901 caccacgccg gtcgcgcgca tcgcggcggc gtccgacccg gcgcgcggct cggtcagcgc
      961 gaagcacggg atctcgtcgc cggccgcgag ccgcggcagg tagaaccgct tctgctcggg
     1021 cgtgccgtag cgcagcagca gctccgcggg cccgagcgag ttcggcacca tgacggtgac
     1081 gcacgcggcg gcgctgcgcg acgcgagccg ggcgaccacc gcgctgtggg cgatcgccga
     1141 gaacccgagc ccgccaaact cctcggggat gatcatgccc aaaaaccgct cacgcttgag
     1201 gaagtgccac acctcggcgg gcagatcgcc ggcggcgcgg atcgcgtgat cgtcgagcag
     1261 gcggcacagg tcggtgcacg gtccatcgag gaacatccgc tcgcgctcgg acagcggccg
     1321 cggcacaaac cgcacgatgc gctcccagcg cggagcgccc gagaacagct cggcgtccca
     1381 ccacaccgta cccgcctcga gggcgatccg ctcggtatcg ccgatccgcg gcccgcgcgc
     1441 cgacagccag cgcatcatgg gtgcgctgag cgcgcgcctg cgaatcgcgg gcacgccggt
     1501 cgcgatcgcg accccgagcc acaccgccat cgcgatcgcc agcccccagc cgccgccggc
     1561 ggcggcccac cgggcgagca gcgggaccca gccgatgacc caggcccgcc acggccagcc
     1621 ggcgcacagc agcgcgatcg caacgacgat ggcgatgacg atcgaggcga gcgtccacat
     1681 gcccggcggt ggtgcaaccg gcgcgcccta cgagcgcctc gcagccgcgc gcgccggcgt
     1741 ctcggctact gcatgcagct gacggtgagg ctcagggcgt cgaggtagaa gctggtcggc
     1801 atgctggtgt cgagcgtcga gcggatccgc agcgtcgcgg tcaccggatc gccggtcagc
     1861 gcggcggtca acgtgagcgc cttgaacaca cacggcgtcg cgccctgctg gttggtcatc
     1921 ttgccgagcg ccgcgatcac cgtggcgccg tcgagcagat cgagctgcag gacgtcgtga
     1981 tcggcggcct gggtctcctt ggtgtcgatg cagatctggc cggacagggt gaccgacttg
     2041 gcgccgtcgg gcagcgggac ctgctggctc agggtctgga cggtgccgtc ggtgccgccc
     2101 aggcacccgg cctgcgtacc gtcgaccggc gtgatcttgg gcgggccgca gatcagcgcc
     2161 gtggtgaccg ggtcctgcat ccaggccggc gtggcggcgt cgaagctgcc gttcggtagc
     2221 acctggacgg gctgagcacc gcacgcgacg tcgcgcttgg ccgcgacatc gccgcaggcg
     2281 gggagcgcga cggcgagcgc cgcgacggtc agagcacacg atgacatggc ccgggaagcc
     2341 tacaccacgt ccgccgcgcg gaccgtgagg ccatcgttcg cgatgcagat cacgcccggg
     2401 gcggaccgcc gcaggagcgc gatcgctaca ggcgcgaaga gtgccggccg gtgtccacgg
     2461 cgtcgcgaat tgggcgaccc gctccgcgag aacacgacag cctggtgagc gtccgcgagc
     2521 gcggcgcgga cgactgcact gcggcgtccg gggcgccgat gagatgtggt aggttggcgg
     2581 atggggaggc cagcggtgtg cgtgatcctg tgcgcgagcc ggccgcgcca cccgtatctc
     2641 ggagcagcgt caggtcgagg acatcccgat cggtctggac gcgtcgatcg caagcgcgtg
     2701 ggagcgaatc gcgatcgatc cgctggtcgt gaacggactc ggcatcctgt gcaccgcgcg
     2761 cgacgcgctc accctggacg agctcggccg ggtggccggc tggaccagcg agccgcagcg
     2821 ccggacgttc ctgggcaggg cgcgcgagct gctcatcgag acgcgacgca cgagccgcgt
     2881 gttcgagtac cggctgcacc acgactcgat ccgcgcgtac atcgccgatg cggtcgggag
     2941 cgacgcgctc gcggcgcatc acctcgcgct ggcgcagcgg ctcgctacct ggccagctcc
     3001 tgcggacgct ggagcacggc gatatgcgct gcgccacgcg ttgctgcatc gcgccgaggc
     3061 aggtgcatgg gccgacgcct ggcgcgtggc cgcggacatg gcgtttctgg aagtgaagtg
     3121 tcgcgcgctc ggcgcgcacg acgccgaggc cgacgtggaa cgaacggcca cgaggtgtcg
     3181 cgcgagcggc gacgacgcgc acggcgaccg cctcaccgac ctggcaaggg cgctcggtcg
     3241 cgagtcgcac tggctgcgag cagcgccgaa ggcaaccgcg gcgctggtct ggaacgggct
     3301 gcaccggttg gggtggtgcg caagcgacct ggatgcgcaa ctccgggtgc cagaaggtag
     3361 ggagtttctg cgcgtgcgcc acgccgcgac gcgcgagagc ccagcgctgg ttcgtagcct
     3421 cgaaggccac acccacgcgg tgatggcgtg cgcgccgacg ccggacggcc ggcacgcggt
     3481 ctcggcgtcg tctgaccaca cgctcaaggt ctgggagctg gcgagcgggc gcccggtggc
     3541 cacgctccaa ggccacaccg acagggtgac ggcgtgcgcg gtgacgccgg acggccggca
     3601 cgtggtctcg gcgtcggatg accacacgct caaggtctgg gacctcggga cctatacagc
     3661 ggcgcgaccg cggtgtacgc cgacctcggc gatcccgacc gcgggctcga cctgatcgag
     3721 cgccacgcga tcagcttcct ctatgcgatc ccgagctacg tccaggcgct gatcgacgcg
     3781 cagcgccgcc ggccgcgccg gatcgacgcg ctgcgcaccg tggtcaccgg ctcggcggcg
     3841 gtccccccgc acctgatcgg cctggcgcgc gacgtgctcg gcgtccgcgt ccagaccctg
     3901 tggggcatga ccgagatcgg cggcgcgacg ttcacctggc ccgacgatcc ggcggactgg
     3961 ccggcgcaca gcgacggccg gccgatcgag tggatggaga tcgacatcgc cgacgaggcc
     4021 gacggcgtcg gccggctgcg ggtgcgcggc gccaaccagt gcctgggcta ccaccagcgg
     4081 cccgacgtgt acgccgcggc gatcgccggc gacggctggt tcgacaccgg cgacctggtg
     4141 cggcgcgacg gccgaggcgg gatccgcatc gtcggccggg tcaaggacgt gatcgtgcgc
     4201 aacggccaca aggtgccggt ggtcgaggtc gagtccgcgc tggccgccca cccccaggtc
     4261 gcgatggtcg cgctggtccc cgaccccgac gcgcagctcg gcgagcggat ctgcgccgtc
     4321 gtcgtgccgc gcgcgccggc gccgacgctg accgagctcc gcgaccacct gcgcgcgctc
     4381 ggcatgtcgg cgcaatactg gcccgaccgg ctccacctgg tgcgcgagat gccgctgacc
     4441 gcgaacggca agatccagaa ctatgtgctg gccgaactgc tgcgcgcgcc cggcccggtg
     4501 ccatgaactt cgcgcccggc gacgaccagc ggctgatcca gcgcaccgcg cgcgaggtcg
     4561 ccgaccgtct gctcgccccg cgcgccgccg cgcgcgatgc gtccggcgag ttcccggtcg
     4621 ccgagctcgc cgagctcgcc cgactcggcc tgctcggcat cac
//
LOCUS       DASVOR010000090         2965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_117869,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000090 DASVOR010000000
VERSION     DASVOR010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117869"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF516_01975"
     CDS             <1..183
                     /locus_tag="VF516_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106459.1"
                     /translation="LRAPSAYAAITAQLAAALDDSFARDPIFGTAAVESALALSSRGR
                     TLARTPASPRFPIALE"
     gene            complement(206..877)
                     /locus_tag="VF516_01980"
     CDS             complement(206..877)
                     /locus_tag="VF516_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106460.1"
                     /translation="MVTLAGCGGGNKVSNDAAIDTAIDTPIDTVPPTLDCTAYCSEIQ
                     KSCAGANAQYPNLDQCTHTCASFVVGTSTVTDTAGNTLGCRINYAVAASTMPGTHCPH
                     AGPAGDLITASVPGFCSGGDVCTSFCTLELLACGSMAAPLPGNPTDSMGNPVFQYRNL
                     GDCMRLCPMWDKTHNYSTMSMGDSLACRLSAAVTAAISIDSAKIFCGYTADAPTGQCA
                     GTASP"
     gene            complement(943..2790)
                     /locus_tag="VF516_01985"
     CDS             complement(943..2790)
                     /locus_tag="VF516_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106461.1"
                     /translation="MHRSKHKHSLRIRGLAIAVVAIAAGGAVIGSCVFDTRTNFCEQF
                     GVRCKEGQECAANQAVCINIGGCGDGIVDKDKGEVCDDGNIVDGEPDPNDPKKIIPDQ
                     CSHDCTSTQQCGNKIIDVGEECDDGSGSNGSPTGTCDSTCHKVSRVCGNGVVDQDTPE
                     ECDPGPMDSAGCNGSAAGAVLGCKTSRCGDGYMNTAAGEKCDSSGVDNLMCNGRICTL
                     PACGDNYINMAAGEQCESNGQDTQTCNGSAAASLACHSPSCGDGYINISFTPSGGLNP
                     EQCDNAGGADSMTCNGNNGGRNGPGSCRNSACGDGYKNTQAGEQCDTQGGADSLTCNG
                     SGAGAVKCKLSACGDGYQNNAAGEQCETAGGSDTSSCNGSAAGAVACHTARCGDGYAN
                     AKVGERCDNGSADSLTCNGTSAPSNLRCQSVVCGDGYKNTAAEDCDPGTGSDTNLCNG
                     SGAGSASCKFATCGDGYVNGRAGEKCDPGGTGSDTSMCNGTGAGSVACLLPTCGDDYV
                     NMKAGEQCEPGGNGSDTSACNGTSAGSAACLAPKCGDGHVNMKAGEQCEPGGNGSDTS
                     ACNGTGAGSAACLVPKCGDGHINTAAGEQCEADTDCASPKTCNSSCKCV"
BASE COUNT          419 a         1019 c          965 g          562 t
ORIGIN      
        1 ctgcgcgccc cgtcggccta cgcggcgatc accgcgcagc tcgccgccgc gctcgacgac
       61 agcttcgcgc gcgatccgat cttcggcacc gccgcggtcg agtccgcgct cgcgctgtcg
      121 agccgcggcc ggacgctggc ccggactccg gcgtcaccgc ggttcccgat cgcgctggag
      181 tgacaggccg gcgggcgcgc gcagctcagg gcgaggctgt gccggcgcac tgcccggtcg
      241 gagcgtccgc cgtgtatcca cagaagatct tcgcgctgtc gatcgagatc gctgcggtga
      301 ccgcagcgct caggcggcac gccagcgagt cccccatcga catcgtactg tagttgtgcg
      361 tcttgtccca catcggacag agccgcatgc agtcgccgag gttccggtac tggaacaccg
      421 gattgcccat ggagtccgtg gggttgccgg ggagcggcgc agccatcgag ccacaggcca
      481 agagctcgag agtgcaaaag ctcgtgcaca cgtcgccacc gctgcagaac cccggcacag
      541 acgccgtgat cagatcgccg gcgggacccg catgggggca gtgagtgcct ggcatcgtcg
      601 aggcagcgac ggcatagtta atccggcacc cgagcgtgtt gccggccgta tcggtgaccg
      661 tactggtccc cacgacgaac gacgcgcatg tgtgggtgca ctggtccagg ttggggtact
      721 gggcgttcgc gccggcgcag ctcttctgga tctcggagca gtacgccgtg caatcgagcg
      781 tgggcggtac cgtgtcgatc ggggtgtcga tcgcggtatc gatcgcggca tcgttggaca
      841 ccttgttgcc gccgccgcag ccagccagag taaccaagat aaggatggtt cgcatgggtc
      901 gaatggggcc gaggttgaac gcggactgcg atggcttcgt catcaaacgc atttacacga
      961 tgagttgcag gtcttgggag aggcgcaatc ggtgtccgcc tcacactgct cgcctgcggc
     1021 cgtattgatg tgtccgtccc cgcacttcgg gaccaggcaa gccgccgagc cagcgccggt
     1081 gccgttgcac gcgctggtat cggatccgtt ccctcccggc tcgcactgtt cccccgcctt
     1141 catgttgaca tggccgtccc cgcacttcgg ggccaggcaa gccgccgagc cagcactggt
     1201 gccattgcac gcgctggtat cggatccgtt ccctcctggc tcgcattgct cccccgcttt
     1261 catgttgacg tagtcgtcgc cgcacgtggg gagaagacaa gccaccgagc cggccccggt
     1321 gccattgcac atgctggtgt cagatcctgt acctcctgga tcgcattttt caccggctct
     1381 cccgttgacg tagccgtcgc cgcacgtcgc gaatttacag ctggccgagc ctgcgccact
     1441 gccattgcag agattggtgt cggatccggt tcctgggtcg caatcctcgg ctgcagtgtt
     1501 cttgtagccg tcgccgcaca ccaccgactg acacctcagg ttcgagggcg ccgaggtgcc
     1561 gttgcaagtg agcgagtcag ccgacccgtt gtcacagcgc tcccctactt tggcattcgc
     1621 gtagccgtca ccacacctgg ccgtgtggca ggccaccgcg ccggctgcgc tgccgttgca
     1681 gctggacgtg tcggagcccc cggcagtctc gcattgctcg ccggctgcgt tgttctgata
     1741 gccgtcgccg caggcggaca gcttgcattt caccgctccc gcaccgctgc cgttgcacgt
     1801 cagcgagtca gcgcccccct gggtgtcgca ttgctccccg gcctgggtgt tcttgtagcc
     1861 gtccccgcac gccgagttac ggcagctacc ggggccgttg cgaccgccgt tgttgccgtt
     1921 gcatgtcatc gagtcggcgc cgcctgcgtt gtcgcactgc tcggggttca gcccgccgct
     1981 gggcgtgaac gagatgttga tgtagccatc gccgcacgac ggggaatgac aggcgagcga
     2041 ggctgcggcg ctgccgttgc aggtctgggt gtcctggccg ttgctctcgc actgctcgcc
     2101 ggcggccatg ttgatgtagt tgtcgccgca cgcgggcagc gtgcagatcc gaccgttgca
     2161 catcaggttg tcgacgccgc tggagtcgca cttctcgccg gcggccgtgt tcatgtagcc
     2221 gtcgccgcac cgcgaggtct tgcagccgag gaccgcgccg gcagcgctgc cgttgcagcc
     2281 cgccgagtcc atcgggcctg gatcgcattc ctcgggggtg tcctggtcga ccacgccgtt
     2341 gccgcacacc cggctcacct tgtgacaggt gctgtcgcac gtgccggtcg gcgagccgtt
     2401 gctgccgctg ccgtcgtcgc attcctcgcc gacgtcgatg atcttgttcc cgcactgctg
     2461 ggtcgacgtg cagtcgtggc tgcactgatc ggggatgatc ttcttggggt cattgggatc
     2521 gggctcgcca tcgacgatgt tgccatcgtc gcagacctcg cccttgtcct tgtccacgat
     2581 gccgtcgccg cagccgccga tgttgatgca caccgcctgg ttggccgcac actcctggcc
     2641 ttccttgcag cgcacgccga actgctcgca gaagttggtc cgggtgtcga acacgcagga
     2701 tccgatcacc gcgccgcccg cggcgatcgc gaccactgcg atggccagtc cccggatcct
     2761 gagcgaatgt ttgtgcttcg atcgatgcat ggcagttctc ctgctcagcg gctcaccgtc
     2821 acgaggacgc cgagcatgtc gctggacacc acgggaacca tggcgacccc ggaggcgccc
     2881 gaggtcgcgt cctcccgctc catcaggcgc ggccggttcc tgtacaccag caccgcgccg
     2941 gcggcgatca gcgcgccgcc cgcga
//
LOCUS       DASVOR010000091         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000091 DASVOR010000000
VERSION     DASVOR010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VF516_01990"
     CDS             <1..1635
                     /locus_tag="VF516_01990"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HEX8106462.1"
                     /translation="SGARASDDSDGAPTPGDGDRDADSDDEPRTPLAQIPPTPEGREA
                     KVRADLAKTYAARFTRLRHPGPFDPASELVNAVASSLDPHTTYLPPADKANFDIRMSG
                     SLEGIGALLSEKDDYVEVREIVPGGASWRHGGLSPGDLILSVAQENQDPVDTVDMRLD
                     DVVKMIRGPKGTVVALRIQKPTGEQQSISITRDVVVVEEAYARGAVLTRKGQRPFGYI
                     HLPSFYGGRGPGQRTAGRDVAKLLDEMKALKVAGVVLDIRGNGGGLLGEAVTMTGALI
                     DRGPVVQVQDSRGNRETYSDDQPGTDYDGPVVVLVDRFSASASEILAGALQDYHRAVI
                     VGTGPTHGKGTVQTLADLDRVTRSNVDLGVLKITVQQFFRVSGSSTQREGVTPDILLP
                     DPAGHIETGERTLEHAIPWSQIPAVDHDSWPATWKTSALVERSTARVTKQPVLAKMVA
                     LTQVLKATREDTRIPLEHAAWEAHRKKQRAAFEAASPDLKAAPSVFTVKSLDDPNLPP
                     PGPGGRHNDPLVRWRETVSRDPWIEECVNILGDMAK"
BASE COUNT          274 a          637 c          680 g          213 t
ORIGIN      
        1 agcggcgccc gggcgagcga cgacagcgat ggcgcgccga cccccggcga tggcgatcgc
       61 gacgccgaca gcgacgacga gccgcggacg ccgctcgcgc agatcccgcc gacgccggag
      121 ggccgcgagg ccaaggtgcg cgccgatctg gccaagacct acgcggcgcg gttcacccgg
      181 ctgcgccacc ccgggccgtt cgacccggcg tccgagctgg tcaacgcggt cgcgtcgtcg
      241 ctcgatccgc acacgacgta cctgccgccc gccgacaagg cgaacttcga catccggatg
      301 agcggctcgc tcgagggcat cggcgcgctg ctcagcgaga aagacgacta tgtcgaggtc
      361 cgcgagatcg tgccgggcgg cgcgagctgg cgccacggcg ggctgtcgcc gggcgacctg
      421 atcctgtcgg tcgcgcagga gaaccaggac ccggtcgaca cggtcgacat gcggctcgac
      481 gacgtggtca agatgatccg cggcccgaag ggcaccgtgg tggcgctgcg gatccagaag
      541 ccgaccggcg agcagcagtc gatctcgatc acgcgcgacg tggtcgtggt cgaggaggcc
      601 tacgcgcgcg gcgcggtgct gacccgcaag ggccagcgcc cgttcggcta catccacctg
      661 ccgagcttct acggcggccg cggcccgggc cagcgcacgg cgggccgcga cgtggccaag
      721 ctgctcgacg agatgaaggc gctcaaggtc gccggcgtcg tgctcgacat ccgcggcaac
      781 ggcggcggcc tgctcggcga ggcggtcacg atgaccggcg cgctgatcga ccgcgggccg
      841 gtggtccagg tccaggacag ccgcggcaac cgcgagacct actccgacga tcagccgggg
      901 accgactacg acgggccggt cgtggtgctg gtcgatcggt tcagcgcgtc ggcgtcggag
      961 atcctcgccg gggcgctgca ggactaccac cgcgcggtga tcgtcggcac cggcccgacc
     1021 cacggcaagg gcaccgtgca gacgctcgcc gacctcgacc gcgtgacgcg gagcaacgtc
     1081 gacctcggcg tcctcaagat caccgtgcag cagttcttcc gggtcagcgg ctcgtcgacc
     1141 cagcgcgagg gcgtgacgcc cgacatcctg ctgccggatc cggcgggcca catcgagacc
     1201 ggcgagcgca cgctggagca cgcgatcccg tggtcgcaga tcccggcggt cgatcacgac
     1261 agctggccgg cgacctggaa gaccagcgcg ctggtcgagc gcagcacggc gcgggtcacc
     1321 aagcagccgg tgctggccaa gatggtggcg ctgacccagg tgctgaaggc gacccgcgag
     1381 gacacccgca tcccgctgga gcacgcggcg tgggaggcgc accgcaagaa gcagcgcgcg
     1441 gcgttcgagg cggcgtcgcc cgacctcaag gcggcgccgt ccgtgttcac ggtcaagtcg
     1501 ctcgacgatc cgaacctgcc gccgccgggg ccgggcggcc gccacaacga tccgcttgtg
     1561 cgctggcgcg agaccgtgtc gcgcgatccg tggatcgagg agtgcgtgaa catcctcggc
     1621 gacatggcga agtagcttcg ccgcgggcgc ggggcgctac gccgacgtcg acgtcgagta
     1681 cgtgccgttg ccggtgctgg tcagctgctg cggcacgccg tcgacggtga tctggggctg
     1741 ccagggcggg acggcggcgg gcgcggacgc gggcgccgcc gctgcggccc gggcgccgcg
     1801 atgg
//
LOCUS       DASVOR010000092         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_322419,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000092 DASVOR010000000
VERSION     DASVOR010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322419"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VF516_01995"
     CDS             <1..408
                     /locus_tag="VF516_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106463.1"
                     /translation="RWAPTAWNQQVPGPFRDEPSLTLAGDVVVTGPTASGAGVEAFGM
                     HELQPSGRHTVVGVRGGMAIECLPGRLRLRGGSYWEPGRFDGVPGRTHATFGIDLRVL
                     AFQLFGERRGRISLTGDVAERYRNLAVSIGFWH"
     gene            complement(516..866)
                     /locus_tag="VF516_02000"
     CDS             complement(516..866)
                     /locus_tag="VF516_02000"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8106464.1"
                     /translation="MKLLVDTHVWLWMLTAPERMNQAARDAIVDPANEVIVSVASAWE
                     VAIKHALGKLPLHAPPARLVEISVTMLAVNVLPIALDHALAAAALPPPSSRSIRSHAR
                     GASSARWLDARYGG"
     gene            complement(863..1117)
                     /locus_tag="VF516_02005"
     CDS             complement(863..1117)
                     /locus_tag="VF516_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106465.1"
                     /translation="MAQALKFHAVVSGKTLSLPDLSAFEGKRVEVIVVEEDGPAEIGA
                     TPRGQRPLGLLRGQFVVPDDFDAPLPPEIQRYFDGDADEP"
     gene            1333..>1855
                     /locus_tag="VF516_02010"
     CDS             1333..>1855
                     /locus_tag="VF516_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106466.1"
                     /translation="MDPADRSRGRRGLQTAAFELDPPSGPALFTISGIGLVASATWLP
                     HGESFRLAAALDGAIDGGLVEADACDPDNCMGYILPARARTPARLVAGAAYRWAPTAW
                     NQQVTGPFRDELSLTLAGDIVVTGPTASGAGVEAFGMHELQPSVATPWSACAAAWPSS
                     ACRAGCGCAAARTG"
BASE COUNT          254 a          650 c          666 g          285 t
ORIGIN      
        1 cgctgggccc cgaccgcgtg gaaccagcag gtgccgggac catttcgcga cgagccgtca
       61 ctcaccctcg ccggcgacgt cgtggtcacc ggcccgaccg cgagcggcgc cggcgtcgag
      121 gcgttcggca tgcacgagct ccagccgtcg ggccgccaca ccgtggtcgg cgtgcgcggc
      181 ggcatggcca tcgagtgcct gccgggccgg ctccggctgc gcggcggctc gtactgggag
      241 ccggggcggt tcgacggcgt ccccggccgg acccacgcga cgttcggcat cgacctgcgc
      301 gtgctggcgt tccagctgtt cggcgagcgg cgcggccgga tctcgctgac cggcgacgtc
      361 gccgagcgct accgcaacct cgccgtgagc atcgggttct ggcactgagg gcggaaccac
      421 gcactttgtg cagcagccgc tgcacaaacg gcgtcggcga gccggttcca gatcagaccg
      481 cccacaagag cgtgcagccg tactgtcgga gcacgtcatc cgccgtaacg agcgtcaagc
      541 catcgagctg agcttgcgcc acgagcatgc gatcgaatgg atcgcgatga tggggggggg
      601 agggcggctg cggcgagggc atggtccagc gcgataggga ggacgttcac ggcgagcatc
      661 gtcacggaga tctcgacgag ccgagccggg ggggcatgca gcggcagctt gccgagcgcg
      721 tgcttgatcg cgacctccca tgcgctggct acggatacga tcacctcgtt cgccggatcc
      781 acgatcgcgt cgcgagcagc ctggttcatc cgctccggtg ccgtgagcat ccacagccag
      841 acgtgggtat ccacgaggag cttcacggct cgtcggcgtc gccgtcgaag taccgctgga
      901 tctcgggtgg cagcggcgca tcgaagtcat cgggaacgac gaactgtccg cgcaatagcc
      961 cgagcgggcg ctgtcctcgc ggtgtggctc cgatctcggc tggcccatct tcctcgacga
     1021 cgatgacctc cacgcgcttg ccctcgaacg cactgaggtc cggcagggac aacgtcttcc
     1081 cagaaacgac ggcgtggaac ttcagcgctt gtgccatgga gcctgccttg cgattgcagc
     1141 atgcacagtg taccgcgtga gcctggctca tgctgaacct ggcggcgggt caggtgcggc
     1201 tacgaccgcc ggccgcgcag cttgaaccgc tggatcttgc cggtcgcggt cttgggcgct
     1261 ggtccagcag gcgccggtgg tcggctcgtc gctcaccgcc aacgcgcggc ggtttcgcct
     1321 cgtgctcgcc cggtggatcc cgcagatcga tctcgcggtc ggcggggcct gcagaccgcg
     1381 gcgttcgagc tcgatccgcc gtccggcccc gcgctgttca cgatctccgg catcgggctg
     1441 gtcgccagcg cgacctggct gccccacggc gagagcttcc ggctcgcggc cgcgctcgac
     1501 ggcgcgatcg acggcggtct ggttgaggcc gatgcctgcg atccggacaa ctgcatgggc
     1561 tacatcctgc cggcccgcgc gcgcaccccc gcgcggctgg tcgcgggtgc cgcgtaccgc
     1621 tgggccccga ccgcgtggaa ccagcaggtg acggggccgt tccgcgacga gctctcgctc
     1681 accctcgccg gcgacatcgt ggtcaccggc ccgaccgcga gcggcgctgg cgtcgaggcg
     1741 ttcggcatgc acgagctcca gccgtcggtc gccacaccgt ggtcggcgtg cgcggcggca
     1801 tggccatcga gtgcctgccg ggccggctgc ggctgcgcgg cggctcgtac tggga
//
LOCUS       DASVOR010000093         2620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_176278,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000093 DASVOR010000000
VERSION     DASVOR010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2620
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176278"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1705)
                     /locus_tag="VF516_02015"
     CDS             complement(<1..1705)
                     /locus_tag="VF516_02015"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8106467.1"
                     /translation="MTISSKPRQTMIIIGAGLGGLSTGCYAQMNGYRSRVFELHDLPG
                     GCCTGWKRGEFTFDGCMSWLLGSGPGNELYQIWLELGALHGKEIRHFEMFNAVETTRG
                     QTVRFYSDPDRLYDHLTELSPVDSKRIRDFCHGLRQFQKCVAAYPFLKPIGLMGKLER
                     LRMYLRFAPFYRVITRSIATRMADYAARFASPVLREAFNYVLYENAPERSLLPFYFPL
                     AYHANRAAGVPEGGWLGVARSIEARYLSLGGQVRYRACVEEILVDHDRAIGVRLGDGQ
                     SHFADIVVVAGDGHRELTELVRGKQPSEAHPELCTDLIEDPDGTALGQFTLFLGLDRR
                     FPGAEHCTTHLLTEDEAAELPGIAHPSIHVQFRNRHYPELSPPGSSVIFASYACDVAR
                     WRALNHGEDRLGRQRKGIEVRTPPGKGPGKRMRSYPQEKRRVAEFLTDYLDRKYPGLA
                     RALVVRDVSTPLTQLRCAGNRSDAALPWQPFGDGGDLLDSEVSRNGAAVSGLRDLYVA
                     EAGGATGGLIRAAAAGRHVLQFICRDDRKRFVAWVDDSAAPPAHVIVPPGGPRMTGPD
                     RA"
     gene            complement(1702..>2620)
                     /locus_tag="VF516_02020"
     CDS             complement(1702..>2620)
                     /locus_tag="VF516_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8106468.1"
                     /translation="LAVLQAGAAYLVVDPADPDDRIAAMIARAPAPVAEGAGLDRTAC
                     VFCAPGSTGEPRAIAITHHGLASAYHAWDVTYRLRSEARVHLQTARAALDRFTGDLVR
                     ALCSGGALCLVGEPVVRDVARLYDTLVHHAVDTAELTPAIARELAAYCVREGKRLDFL
                     RLVVIGPDAWKVEDDRQLRALLGRRSRLLHVYGVTEATIDSAYFEGPVDGLEPDQLVP
                     IGGPFPGSRVFVLDRRGAPVPAGVPGELWVGGNGVAAGYLGDRDATAARFTTVELDER
                     GPMRLYRTGDIARWDARGTLHAVRTRGEP"
BASE COUNT          371 a          955 c          948 g          346 t
ORIGIN      
        1 gcgcgcgatc gggcccggtc atgcggggcc cgcccggcgg gacgatcacg tgggccggcg
       61 gcgcggccga gtcatcgacc caggcgacga accgcttgcg gtcgtccctg caaatgaatt
      121 gcaacacgtg gcggcccgcc gcggcggcgc ggatcagccc gccggtcgcc cccccggcct
      181 ccgccacgta caggtcgcgc agcccggaga ccgccgcgcc gttccgggac acctcgctgt
      241 ccagcagatc gccgccgtcg ccgaacggct gccacggcag cgccgcgtcg ctgcggttcc
      301 cggcacagcg cagctgggtg agcggggtcg acacgtcgcg gaccacgagc gcgcgggcga
      361 ggccggggta cttgcggtcg aggtagtcgg tcaggaactc cgcgacccgg cgcttctcct
      421 gcgggtagga ccgcatgcgc ttgccggggc ccttgccggg gggcgtgcgg acctcgatgc
      481 ccttgcgctg ccggcccagc cggtcctcgc cgtggttcag cgcccgccag cgcgcgacgt
      541 cgcacgcata gctcgcgaag atcaccgagc tgccgggggg agacagctcg gggtagtgcc
      601 ggttgcgaaa ctggacgtgg atgctggggt gcgcgatccc gggcagctcc gcggcctcgt
      661 cctcggtcag caggtgggtc gtgcagtgct cggctccggg gaaccgccgg tcgaggccga
      721 ggaacagcgt gaactggccc aaggccgtgc cgtccggatc ctcgatcagg tcggtgcaga
      781 gctccgggtg ggcctcgctc ggctgcttgc cgcgcaccag ctccgtgagc tcccggtgcc
      841 cgtcgcccgc gaccaccacg atgtcggcga agtggctctg gccgtcgccc agccggaccc
      901 cgatcgcgcg gtcgtggtcg accaggatct cctcgacgca cgcgcggtag cgaacctgcc
      961 cgccgagcga caggtaacgg gcctcgatcg agcgcgcgac gccgagccag ccgccctcgg
     1021 ggacgccggc cgcgcggttg gcgtggtacg cgagcgggaa gtagaacggg agcagcgacc
     1081 gctcgggcgc gttctcgtac agcacgtagt tgaacgcctc gcgcagcacc gggctcgcga
     1141 accgcgcggc gtagtcggcc atccgggtcg cgatcgaccg cgtgatcacg cgatagaacg
     1201 gcgcgaaccg caggtacatc cgcagccgct cgagcttgcc catcagcccg atcggcttga
     1261 ggaagggata cgcggcgacg cacttctgga actgccgcag cccgtggcag aagtcgcgga
     1321 tccgcttgct gtcgaccggc gacagctcgg tcagatggtc gtagaggcgg tcggggtccg
     1381 agtagaaccg cacggtctgg ccacgcgtgg tctcgaccgc attgaacatc tcgaagtggc
     1441 gaatctcctt gccgtgcagt gcgccgagct cgagccagat ctgatacagc tcgttacccg
     1501 ggccgctgcc gagcagccag ctcatgcacc cgtcgaaggt gaactcgccg cgcttccagc
     1561 cggtgcagca gccgcccggg aggtcgtgca gctcgaacac gcggctgcga tatccattca
     1621 tctgcgcata gcacccggtg gacagcccac cgaggcccgc gccgatgatg atcatggtct
     1681 gtcgcggctt gctcgagatc gtcatggctc accccgcgtc ctcaccgcgt gcagcgtccc
     1741 gcgcgcgtcc caccgcgcga tgtcgccggt gcggtacagc cgcatcggcc cccgctcgtc
     1801 gagctcgacc gtcgtgaacc gggccgcggt ggcgtcgcga tcgccgaggt agccggcggc
     1861 gacgccgttg ccgccgaccc acagctcgcc gggcacgccg gccgggaccg gcgcgccgcg
     1921 gcgatccagc acgaacaccc ggctgcccgg aaacggcccg ccgatcggga cgagctggtc
     1981 gggctcgagg ccatcgaccg ggccctcgaa gtacgcgctg tcgatcgtcg cctcggtgac
     2041 gccgtagacg tggagcaggc gcgatcgccg cccgagcagc gcgcggagct gccggtcgtc
     2101 ctcgaccttc caggcgtccg gaccgatcac gacgagccgc aggaagtcca gtcgcttgcc
     2161 ctcgcgcacg cagtacgccg ccagctcgcg cgcgatcgcc ggcgtgagct cggcggtgtc
     2221 gaccgcgtgg tggaccaggg tgtcgtacag ccgggcgacg tcgcgcacga ccggctcgcc
     2281 gaccagacac agcgccccgc cggagcacag cgcgcgcacc aggtcgccgg tgaaccggtc
     2341 gagcgcggcg cgcgcggtct ggaggtggac gcgcgcctcg ctgcgcagcc ggtaggtcac
     2401 gtcccatgcg tggtacgccg aggccaggcc gtggtgggtg atggcgatcg ccctgggctc
     2461 gcccgtcgat cccggcgcgc agaacacgca cgcggtgcga tcgaggccgg ccccctccgc
     2521 gaccggcgcc ggcgcccgcg cgatcatggc cgcgatgcga tcatcggggt ccgccgggtc
     2581 caccaccagg tacgcggcgc cggcctggag gaccgcgagc
//
LOCUS       DASVOR010000094         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410137,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000094 DASVOR010000000
VERSION     DASVOR010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410137"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VF516_02025"
     CDS             <1..840
                     /locus_tag="VF516_02025"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX8106469.1"
                     /translation="AAAGARAKLIKDVAEAGAEAMFKMVGFVMKFAPFGVGAAIAATL
                     GTSGIAVLLPLLKLVATLYGALIVFFVLLFTAMRLLTRVHMGTFLAAIREPALIAFTT
                     ASSEAALPRALQVLEGLGIPRRIVSFVVPTGYAFNLDGSTLYLALAMVFVAQAAHVEM
                     SLRQQLVAMLFLMLSSKGVAGVPRASLVVLAGALTAFDLPADGVALVLGVDAVMDMGR
                     TCVNVVGNCVATVVVAVWENELPTEAPIFWPRAAPGAELSEAAAYAMATGEATAVEPV
                     GVE"
     gene            896..1234
                     /locus_tag="VF516_02030"
     CDS             896..1234
                     /locus_tag="VF516_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106470.1"
                     /translation="MEERSACAQLKRRFEAAGFRIEENQRFDEDGVWFEIDGFDAARR
                     VGYEYLTKEAGDGWDVDGNVIARLAERRQRGELHVLVVDEADAPDAASLDRVIDAFLG
                     ELEAGGVLPR"
     gene            1323..>1955
                     /locus_tag="VF516_02035"
     CDS             1323..>1955
                     /locus_tag="VF516_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8106471.1"
                     /translation="MGDSDILSVAPLGFQWPTLDPFLFCVHHDDAYPAGNEQLGPAAS
                     LDGRDLGMDFAGKDGWRMYHGTTVPGFPQHPHRGFETVTIVRRGLIDHSDSLGATARF
                     GRGDVQWLTAGKGISHCEMFPLLDPAGPNPVELFQIWLNLPRRDKLVAPYFTMLWDRT
                     IPRIEAPGVLVRAIAGALGDATPPSPPPSSWASRGEADVAIWTLHLQPGAS"
BASE COUNT          241 a          715 c          704 g          295 t
ORIGIN      
        1 gcggccgccg gcgcgcgcgc gaagctgatc aaggacgtcg ccgaggccgg cgccgaggcg
       61 atgttcaaga tggtcgggtt cgtgatgaag ttcgcgccgt tcggggtcgg cgccgcgatc
      121 gcggcgacgc tcggcaccag cggcatcgcc gtgctgctgc cgctgctcaa gctggtcgcc
      181 acgctgtacg gcgcgctgat cgtgttcttc gtcctgctgt tcaccgcgat gcggctcctg
      241 acccgcgtcc acatggggac gttcctcgcc gcgatccgcg agccggcgct catcgcgttc
      301 accaccgcgt cgtcggaggc cgccctgccc cgcgcgctgc aggtcctcga ggggctcggc
      361 atcccgcgcc ggatcgtcag cttcgtcgtg ccgaccggct acgcgttcaa cctcgacggt
      421 tcgacgctct acctggcgct cgcgatggtg ttcgtcgccc aggccgccca cgtcgagatg
      481 tcgttgcgcc agcagctcgt cgcgatgctg ttcctcatgc tgtcgagcaa gggcgtggcg
      541 ggggtgccgc gcgcctcgct ggtcgtgctc gccggcgcgc tgaccgcgtt cgacctgccc
      601 gccgacggcg tcgcgctggt gctcggggtc gacgcggtga tggacatggg gcggacctgc
      661 gtcaacgtcg tcggcaactg cgtcgcgacc gtcgtggtgg cggtgtggga gaacgagctc
      721 cccaccgagg cgccgatctt ctggccacgt gccgcgcccg gagccgagct gtcggaggcc
      781 gcggcgtacg cgatggcgac cggcgaggcc acggcggtgg agccggtcgg cgtcgagtga
      841 accgcggccg cgcgccgggc gtgcagtcgc cggcccgctg gcgtacgatg cggccgtgga
      901 agagcgctcc gcctgtgcac agctgaagcg gcgattcgaa gcggccgggt ttcgcatcga
      961 ggagaaccag cggttcgacg aggacggcgt gtggttcgag atcgacgggt tcgacgccgc
     1021 gcgccgggtc ggctacgagt acctcaccaa ggaggcgggc gatggctggg acgtcgacgg
     1081 caacgtgatc gccaggctcg cggagcgccg ccagcgcggc gagctccacg tgctggtcgt
     1141 ggacgaggcc gacgcaccgg acgccgcctc gctcgaccgg gtgatcgacg ccttcctcgg
     1201 cgagctcgag gccggcggcg tcctccccag gtagccgggg cggccggcgc acggccggtg
     1261 cggaacagtc ctcgccaaac ggcgcgattg ccgcggccgg gacctgtgcc cacgctgcca
     1321 gcatgggcga ttccgacatc ctctcggtcg caccgctcgg ctttcagtgg ccgacgctcg
     1381 acccgttcct gttctgcgtc caccacgacg acgcgtatcc ggccggcaac gagcagctcg
     1441 gaccggcggc gtcgctcgac ggccgcgacc tcggcatgga cttcgccggc aaggacggct
     1501 ggcggatgta ccacggcacc accgtcccgg gcttcccgca gcacccccac cgcggcttcg
     1561 agaccgtcac catcgtccgc cgcggcctga tcgatcactc cgactcgctg ggcgccaccg
     1621 cgcggttcgg ccgcggcgat gtccagtggc tgaccgccgg caagggcatc tcgcactgcg
     1681 agatgttccc gctgctcgac ccggccggcc ccaacccggt cgagctgttc cagatctggc
     1741 tcaacctgcc gcgccgcgac aagctggtcg cgccgtactt caccatgctg tgggaccgca
     1801 cgatcccgcg gatcgaggcg cccggcgtgc tggtccgcgc gatcgccggc gcgctgggcg
     1861 acgcgacccc gccgtcgccg ccgccgagct cgtgggcgtc gcgcggtgag gccgacgtcg
     1921 cgatctggac cctccacctc cagcccggcg cgtcg
//
LOCUS       DASVOR010000095         5708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439229,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000095 DASVOR010000000
VERSION     DASVOR010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5708
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439229"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3440
                     /locus_tag="VF516_02040"
     CDS             <1..3440
                     /locus_tag="VF516_02040"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8106472.1"
                     /translation="LGDAGVATAELRQLLVSSPATVAADVARAADAGTIAVVIDQLEE
                     LFTLCGCAEEREQFAAAIAQLAASADLPVRVIATIRDDFLMQLDALPALRALLSPALV
                     LLGNPLHDDLVRIVVEPARRAGYTLSDPELAHDMVSAVASRPGALALLSFTASRLWEL
                     RDRRFRQLTRSAYDAMGGVAGALVRHAEATFEALSADEQRLAREIFRQLVTADGARAQ
                     LTADELRQRLATPRAAAVIDKLVAARLLALSESQTAGESHIEVIHDALLDGWPRLQRW
                     VREDVDGARMRDQLRIAARQWSERGRPRSLVWRDGMLADLERWLRRGKPSALTELEAA
                     FVEASRRLVLRARIRRRIGLVAIGFAIAFAVFGYRTISVHIDESVLEHQLTQGYADWG
                     QEALLDGRPIEALAYLHLAARRGDDSARVKFTLARAAEPFQHEVARLQAAHGRMWWAS
                     YSPDGRRIVTTDDEGAQVWNADSRTLELSLPHGNIVYQAAFTPDGAQIVTAGADGTVK
                     VWDARTGRPIWSLRRGPPGDVATYLTLAISPRGTEVAAIDSTGAVADIWDLRTGAPIR
                     ALAGNAGSTLEYSYDGRWLATSTEADVSVYNTATWERLQIPGNDVTTLSFDPTAQRLA
                     TATARGDVSIWSVPSGERQWHLRESGARVNHVAFSPDGTFVVATSQDGVDRIWNTKNG
                     ELQVELKNHRSASTWAEFDAGSRLVVSVDNAKEVTITDVALRAAVSTLDGPSGLIRAV
                     HFDPKARHVVAASWDGTTRIWNTTRSYLRWATQPLGAACGSPLHGEPDRRFVAVACAD
                     RGTWVWDTQDAAGPRLLAQLPSPTPAPGDFPTAPPAVSAAGDRAAIAMGNVVTIYELP
                     GGRVMSTVHHHAPVTTVAFAPDGHELVSGSVDGSLVVASERDQIEVARLPAAVDVAAL
                     LPGDRVVVADARPRLGVYRVARPAVPLAEQALQTRATTLRSSSDGQRLLAIPRTGAVG
                     PALLWRLDEPSPIWRLDTHNTPVISARFVNDDHAVLTGSGDGTARLWDAQTGRLRRSY
                     SHGGAYVADVAFEPDGAFAVTAGGDGVLRFWDVPSGHLIWVLQAHQSAIIGVHFDGTS
                     IVTRALSGEIARWELSNLLSSQELGVTLDRIVHCLTVRFDPETRGLVEQEQDPRCDG"
     gene            3990..>5708
                     /locus_tag="VF516_02045"
     CDS             3990..>5708
                     /locus_tag="VF516_02045"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8106473.1"
                     /translation="MATGQRRHLLVFGGASPWIYPLPAHGDVVIGRSETADLRIAHGA
                     VSRQHAKLVLDGDAVVLVDLGSHNGTYVNRCRITGRHALRSGDVITIHATTLVLHTTA
                     PVVPSATVLELDALRQQIENELDRTMQSERLFSLLCLVGGMAGDRAAIQRMVVAQLRR
                     VDVAAWSGDGALYVLLPEAGAEEAVAVATRIRGRLDRALRIGHVSCPDDGYDASALIE
                     RVREAAGAAQPGKIAGLDRAFHSLTIGAQRVIVADPAVARLYALIERLAPVALPVLIT
                     GETGCGKELVATAIHARSPRAERPLISLNCAALHEQLVESELFGHEKGAFSGAIASRA
                     GLIETASGSTLFLDEVGELAPSIQAKLLRVLESHRVTRVGDVREREVDVRVVAATNRD
                     LEADVAAGRFRRDLFFRLSAATLHLPPLRQRPRELPLLATAFLDDACRRNQRSPMRIS
                     DEAMAVLLAHSWPGNVRELKNLMQYVAAAIVVDVLRADHLGERLGRLHPEPTQSSIVT
                     VPGVPPRFRPLAEELRELEITRIRQAIEATGGNQTRAAGLLAMPVRTFFEKAKQYGLT
                     PKRKRYD"
BASE COUNT          825 a         1964 c         2078 g          841 t
ORIGIN      
        1 ggctcggcga tgccggcgtc gcgaccgccg agctccgcca gctgctggtc agctcgccgg
       61 cgaccgtcgc ggcggacgtc gcccgcgccg cggacgccgg cacgatcgcc gtggtgatcg
      121 accagctcga ggagctgttc acgctgtgcg ggtgcgccga ggagcgcgag cagttcgccg
      181 ccgcgatcgc ccagctcgcg gcgtccgccg acctgccggt ccgcgtgatc gcgacgatcc
      241 gcgacgactt cctgatgcag ctcgacgcgc tgccggcgct gcgcgcgctg ctgtcgccgg
      301 cgctggtcct gctcggcaac ccgctgcacg acgacctggt ccggatcgtc gtcgagcccg
      361 cccggcgcgc cggctacacc ctgtcggacc ccgagctcgc ccacgacatg gtcagcgcgg
      421 tcgccagccg gccgggcgcg ctcgccctgc tgtcgttcac cgcgtcgcgg ctgtgggagc
      481 tgcgcgaccg ccggttccgc cagctgaccc gcagcgccta cgacgcgatg ggcggggtcg
      541 ccggcgccct ggtccgccac gccgaggcca cgttcgaggc gctgtccgcc gacgagcagc
      601 gcctggcccg cgagatcttc cgccagctgg tcaccgccga cggcgcccgc gcccagctga
      661 ccgccgacga gctgcgccag cggctggcga ccccgcgcgc cgccgccgtg atcgacaagc
      721 tggtcgccgc gcggctgctc gcgctgtccg agtcgcagac cgccggcgag tcgcacatcg
      781 aggtcatcca cgacgcgctg ctcgacggct ggccgcgcct gcagcgctgg gtccgcgagg
      841 acgtcgacgg cgcgcggatg cgcgatcagc tgcggatcgc ggcgcgccag tggagcgagc
      901 gcggacggcc gcgcagcctg gtctggcgcg acggcatgct ggccgatctg gagcgctggc
      961 tgcgccgcgg caagccgagc gcgctcaccg agctcgaggc cgcgttcgtc gaggcgagcc
     1021 ggcggctcgt gctgagggcc cggatccgcc gccggatcgg cctggtcgcg atcgggttcg
     1081 cgatcgcgtt cgcggtgttc gggtatcgca cgatcagcgt gcacatcgac gagagcgtgc
     1141 tggagcacca gctcacgcag ggctatgccg actgggggca ggaggcgctg ctggatggca
     1201 ggccgatcga ggccctggcc taccttcacc tggccgcgcg tcgcggggac gactccgcgc
     1261 gcgtgaagtt cacgctcgcg cgtgcggccg agccgttcca gcacgaggtc gcgaggctcc
     1321 aggccgcgca cgggcggatg tggtgggcgt cgtactcgcc ggatgggcgc cggatcgtca
     1381 cgaccgatga cgagggcgcg caggtatgga acgcggactc gaggaccctg gagctgtcgc
     1441 tgccccacgg gaacatcgtc tatcaggcgg cgttcacgcc ggatggcgcc cagattgtca
     1501 cggcgggcgc cgacgggacc gtcaaggtct gggacgcgag gacgggccgg ccgatctggt
     1561 ccctccgtcg ggggccaccc ggtgacgtcg cgacgtacct cacgctcgcg atctctccac
     1621 gcgggaccga ggtcgcggcg atcgacagca cgggagccgt cgccgacatc tgggatcttc
     1681 ggaccggcgc cccgatccgc gcactggccg gcaatgcagg atccacgctc gagtacagct
     1741 acgacgggcg ctggcttgcc accagcacgg aagcggatgt cagcgtgtac aacacggcga
     1801 cgtgggagcg tctgcagatc cccgggaacg acgtcaccac gttgagcttc gatccgaccg
     1861 cgcaacggct cgccaccgcg accgcgcggg gcgatgtctc gatctggagc gttccgagcg
     1921 gcgaacgcca gtggcacctc cgcgagagcg gcgctcgggt caatcacgtc gcattctcac
     1981 cggacggtac gttcgtcgtg gcgaccagtc aggatggcgt cgatcggata tggaatacca
     2041 agaacggaga gctccaggtc gagctcaaga atcaccgcag cgcttccacc tgggcggagt
     2101 tcgacgccgg ctcgcggctc gtggtgagcg tggacaacgc caaggaggtg acgatcacgg
     2161 acgtcgcgtt gcgcgccgcg gtgtcgaccc tggacgggcc gagtggcctg atccgcgcgg
     2221 tgcatttcga tcccaaggcg cgacatgtgg tcgcagcgtc ctgggacggc acgacgagga
     2281 tctggaacac gacgcggtcg tacctgcgct gggccaccca gccgctgggg gcggcctgtg
     2341 ggagtccact ccatggggaa ccggatcgac ggttcgtcgc ggtggcatgt gcggatcgcg
     2401 gtacctgggt atgggatacc caggatgcgg ccggacccag gctcctcgca cagctgccga
     2461 gccccacgcc ggcaccgggc gactttccca cggcgccgcc ggcggtgagc gccgccggcg
     2521 atcgcgcggc gatcgcgatg gggaacgtgg tcacgatcta cgagctaccc ggcggtcggg
     2581 tcatgagcac tgttcatcat cacgcgccgg tgacgacggt ggcgtttgcg cccgacggac
     2641 atgagctcgt gagcggcagc gtcgacggtt cgctggtggt cgcttccgag cgtgaccaga
     2701 tcgaggtcgc gaggctgccc gccgcggtcg acgtcgcggc gcttctcccg ggggaccggg
     2761 tcgtcgtcgc ggacgctcgt cctcgactcg gggtctatcg cgtggcgcgt cctgccgttc
     2821 ccctggccga gcaggccctg cagacgcggg ccacgacgct ccggtcgtcg tcggacggtc
     2881 aacgcctgct ggcgattccg aggacggggg cggtcgggcc ggcgctgctc tggcgcctcg
     2941 atgagccctc gccgatctgg cggctggaca cgcacaacac accggtgatc tccgcccgct
     3001 tcgtgaacga tgaccatgcg gttctgaccg gaagcggcga cgggaccgcg cggctgtggg
     3061 atgctcagac cggccggctc aggagatcat actcacacgg cggggcatac gtcgccgatg
     3121 tggcgttcga gcccgacggc gccttcgccg tgaccgccgg tggcgacggg gtgctccggt
     3181 tctgggatgt cccttcgggg cacttgatct gggtcctcca ggcccatcaa tcggcaatca
     3241 ttggagttca tttcgatggt acgagcatcg tgacccgcgc cctcagcggt gagatcgccc
     3301 ggtgggagct ctcgaatctg ctgtcgtcgc aggagcttgg ggtcactctg gaccgcatcg
     3361 tccattgcct cacggtgcgc tttgaccctg agaccagggg cctggtcgaa caagagcaag
     3421 atccgcgctg tgacggctag gtgagctgca agtacgatgt tgcagaagtt gcttgcagaa
     3481 ctggcaagct gtgcaagctc cgcacgaaac tgagtctggt aacggcggag aaactgcgcg
     3541 acgctctggg tatgacgctt gcgtcgcgaa gacgtacgct cgtgtcgata tcgccagagg
     3601 aggcccgaca atgaagaagc ttatcgtgag gctcaaagtg cgccgtgaag tggtccatac
     3661 gctgacccgt cgcgatctca cgaccgttgc cggaggagcg aacagcccca acgagaccaa
     3721 ggtcgccggt ggctgcgcgg gcggaacccc cggggagagt cagatagatc cctagtggtc
     3781 gcgacgtagc ccgactcctg tggcgtcgca cgcgacgcgc gtgatgtggc gatcggtcga
     3841 accgctgcgc tgaaagctac ccctggtgag gggggcgacc ttgcattgag gccgcgtaca
     3901 tcggaccatg gagctgaatg ggcgaagtac cggggtcgtg tgcggaggca acgccctctg
     3961 accgcgtcgt acaggtcgag gaagaggtgg tggccacggg gcagcgaagg cacctgctgg
     4021 tgttcggcgg agcgtcaccg tggatctatc cactgccggc gcacggcgac gtcgtgatcg
     4081 ggcgatcgga gaccgcggac ctgcggatcg cgcacggcgc ggtgtcgcgc cagcacgcca
     4141 agctcgtgct cgacggcgac gcggtcgtgc tcgtcgatct cgggtcacac aatggcacgt
     4201 acgtcaaccg ctgccggatc accggccggc atgcgctgcg gtcaggggat gtcatcacga
     4261 tccatgcgac caccttggta ctccacacca cggcgccggt cgtgccgtcg gcgacggtgc
     4321 tggagctcga cgcactgcgc cagcagatcg agaacgagct cgatcgcacg atgcagagcg
     4381 agcggctgtt ctcgctgctg tgtctcgtcg gcggcatggc cggcgatcgc gccgcgatcc
     4441 agcgcatggt ggtcgcgcag ctgcgccggg tcgacgtcgc ggcgtggtcg ggcgacggcg
     4501 cgctgtacgt gctgttgccc gaggccgggg ccgaggaggc ggtcgcggtc gcgacccgga
     4561 tccggggccg gctcgaccgc gcgctgcgga tcggccacgt cagctgcccc gatgacggct
     4621 acgacgccag cgcgctgatc gagcgcgtcc gcgaggccgc gggcgcggcg cagcccggca
     4681 agatcgcggg gctcgaccgc gcgttccact cgctcacgat cggcgcgcag cgcgtgatcg
     4741 tcgccgatcc ggcggtggcg cggctgtacg cgctgatcga gcggctcgcg ccggtggcgc
     4801 tcccggtgct gatcaccggc gagaccggct gcggcaagga gctggtcgcc acggcgatcc
     4861 acgcccggtc gccgcgcgcc gagcggccgc tgatctcgct caactgcgcc gcgctgcacg
     4921 agcagctggt cgagagcgag ctgttcggcc acgagaaggg cgcgttctcg ggcgcgatcg
     4981 cgagccgggc cggcctgatc gagaccgcgt cgggatcgac gctgttcctc gacgaggtcg
     5041 gcgagctcgc cccctcgatc caggccaagc tgttgcgcgt gctcgagtcg caccgggtca
     5101 cccgggtcgg cgacgtccgc gagcgcgagg tcgacgtccg ggtggtcgcg gcgaccaacc
     5161 gcgatctcga ggccgacgtc gcggccggcc ggttccgccg cgacctgttc ttccggctgt
     5221 ccgccgcgac cctgcacctg ccgccgctcc gccagcggcc gcgcgagctg ccgctgctcg
     5281 cgaccgcgtt cctcgacgac gcctgccggc gcaaccagcg gtcgccgatg cggatctccg
     5341 acgaggcgat ggcggtgctg ctcgcgcaca gctggccggg caacgtgcgc gagctcaaga
     5401 acctgatgca gtacgtcgcc gccgcgatcg tggtcgacgt gctgcgcgcc gaccacctcg
     5461 gcgagcggct gggccggctg cacccggagc cgacgcagag cagcatcgtc accgtccccg
     5521 gggttccccc gcggttccgc ccgctcgccg aggagctccg cgagctggag atcacgcgga
     5581 tccgccaggc gatcgaggcg accggcggca accagacccg cgccgcgggc ctcctggcga
     5641 tgccggtccg gacgttcttc gagaaggcca agcagtacgg tctgacgccc aagcgcaagc
     5701 gctacgac
//
LOCUS       DASVOR010000096         3814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_380797,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000096 DASVOR010000000
VERSION     DASVOR010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3814
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380797"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1376
                     /locus_tag="VF516_02050"
     CDS             <1..1376
                     /locus_tag="VF516_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106474.1"
                     /translation="EPTAGPAAPPAPVTVAPPPSPAQSEKPPESPSVSGKWTTSFYGF
                     AEGDLIYDTVQGATNGGGPTEALGNGALPRPASGTAQMVGQFRALHDQFQTTARNTRL
                     GFRFTAPTVNDLKATGNIEFDFGGNQPNTVSEASLYVNATMRLRHAYFKLETPFLDIM
                     MGQTWQLFGWQPNLQPGSVQFQGLPGWLSSRTAQLRLGKTVKAGDVTVDVQVAATRPG
                     QRGGGLPDGTGGLRLAYDKLKAARIVGGAGSALDSAMIGVSVIGRRFNVTELKAGSTD
                     TVQKNGYGLAVDALLPIVPATKESKANALTLGGEFVTGGGIADLYTGLNGGVSLPATI
                     PGTMPAAAFAPNIDNGLVGYLTSDASVHAVRWQSFGAFAQYFLPPKGNVVVIVNYSHL
                     SSDNTHNFGAANAAFEKQDYVDGDLMFDVTPAVRFGAGFVWLKQTYVDGVEAHDYRGQ
                     LSAMFIF"
     gene            1660..1803
                     /locus_tag="VF516_02055"
     CDS             1660..1803
                     /locus_tag="VF516_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106475.1"
                     /translation="MVAGATSSNELAVLSGFDCLLGVRRVGERRKIDEECGERQANAA
                     FHW"
     gene            complement(1912..2439)
                     /gene="yhbY"
                     /locus_tag="VF516_02060"
     CDS             complement(1912..2439)
                     /gene="yhbY"
                     /locus_tag="VF516_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEX8106476.1"
                     /translation="MQAAAPPRQRPDLTFERDPDRAPGRLACRVEFDPREGPAQAGCS
                     RSSSWHLLSLKVPPEGSFQTARNSPGGLRFRVQTAVLTGKQRRHLRSLGHELKPIVQI
                     GRAGIDDGLVAAVDQALADHELVKIKLGEAAALDRHDAAEAIARQTGSEVAQVLGNTL
                     LLYRADPDDPVIILP"
     gene            complement(2527..2979)
                     /locus_tag="VF516_02065"
     CDS             complement(2527..2979)
                     /locus_tag="VF516_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106477.1"
                     /translation="MSELKKAVDLVREARWESWRRLAEKLPDHHVLAVEMYRVDRHTT
                     VSIFTLVRHGKVVQEVLQGDGRGLNVRPVAPHADRCVPLPMGRSFQVESLMMSSPTAS
                     TGDGDPVAYSIAYGEPPPKQGTEPGIVASGGVTMANTFDTTELLVGGS"
     gene            complement(2992..>3814)
                     /locus_tag="VF516_02070"
     CDS             complement(2992..>3814)
                     /locus_tag="VF516_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8106478.1"
                     /translation="DTTGRYVGTWKSPEVDAAALIPIDLVDRLRACERVAVLTRAPVL
                     GRGRLLPRDMAWSYLLPGPASPASPASSPGTTESPRLVVANPETLPDLKLPPLALYPA
                     ATEPGARVLRGADATPSRVLLAMRDASVIEFHTHGILGNDVSESSYLVLSPELDRQYA
                     LTADDVASVQLAARPLVILGACHAADSSKSLEGGIGLPEAFLRSGARAVIASPDAVGD
                     LGAVGFFAAVRDRVLRGADPAVALRDERLHPSARSHDDAWVAGVVVFERPETVSM"
BASE COUNT          585 a         1318 c         1357 g          554 t
ORIGIN      
        1 cggagcctac cgcgggcccg gcggccccgc ccgctccggt gacggtcgcg ccgccgccgt
       61 cccccgccca gtccgagaag ccgcccgagt cgccgtcggt ctcgggcaag tggaccacgt
      121 cgttctacgg cttcgccgaa ggcgacctga tctacgacac ggtccagggc gcgaccaacg
      181 gtggcggccc gaccgaggcg ctgggcaacg gcgcgctgcc gcggccggcg agtggcaccg
      241 cccagatggt ggggcagttc cgcgccttgc acgaccagtt ccagaccacg gcgcgcaaca
      301 cgcgcctcgg gttccggttc accgcgccga ccgtcaacga cctcaaggcg accggcaaca
      361 tcgagttcga cttcggcggc aaccagccga acacggtgtc cgaggcgtcg ctgtacgtca
      421 acgcgaccat gcggctccgg cacgcgtact tcaagctcga gaccccgttc ctcgacatca
      481 tgatggggca gacctggcag ctgttcggct ggcagccgaa cctccagccg ggctcggtgc
      541 agttccaggg cctgccgggc tggctcagct cgcgcacggc gcagctgcgg ctcggcaaga
      601 cggtcaaggc cggcgacgtc acggtcgacg tccaggtcgc cgcgacccgg ccgggtcagc
      661 gcggcggcgg cctgcccgac ggcaccggcg gcctccggct ggcctacgac aagctcaagg
      721 cggcccggat cgtcggcggc gccggcagcg cgctcgacag cgcgatgatc ggcgtgtcgg
      781 tgatcggccg gcggttcaac gtcaccgagc tcaaggcggg ctcgacggac accgtccaga
      841 agaacggcta tggcctggcg gtcgacgcgc tgctgccgat cgtccctgcg accaaggaga
      901 gcaaggcgaa cgcgctcacc ctgggcggcg agttcgtcac cggcggcggg atcgccgatc
      961 tgtacaccgg gctgaacggc ggcgtgtccc tgccggcgac catcccgggc acgatgcccg
     1021 cagcggcgtt cgcgcccaac atcgacaacg gcctggtcgg gtacctgacg tcggacgcct
     1081 cggtgcacgc ggtgcgctgg cagtcgttcg gcgcgttcgc gcagtacttc ctgccgccga
     1141 agggcaacgt ggtcgtgatc gtcaactact cgcacctgag ctcggacaac acgcacaatt
     1201 tcggcgcggc caacgcggcg ttcgagaagc aggactacgt cgacggcgac ctgatgttcg
     1261 acgtcacgcc ggcggtgcgg ttcggcgccg gcttcgtgtg gctgaaacag acctacgtcg
     1321 acggcgtcga ggcgcacgac taccgcggtc agctgtcggc catgttcatc ttctgatcat
     1381 cttctgatca tcctcgatcg ccctcgggga ggcggtgtcc acggcggccg caatcctcag
     1441 ctgggaggtg acggccacgt cggatccgca gccggtcgcg gacaccgcct ccgttcatcg
     1501 gcggcggcgc cggcggagca cccccagcag cgcgagggcc gtcgcgctcg aggacagtcc
     1561 gcctgccgcc gtgcaaccgc cgggggtctt cccaacccgg ccaacccggc ggacccggcg
     1621 ggttctggat cgtcgctggt gccaggagtg ccggcggtca tggtcgccgg cgcaacgagc
     1681 tcgaacgaac ttgcggtgct ttctggcttt gactgcctgc tcggggtgcg ccgcgtgggc
     1741 gagcgacgca agatcgatga ggagtgtggt gaacgccagg ccaatgctgc gttccactgg
     1801 tgatgatcta tcaaccagat tggacaggat gcaatggctt tttgattcgc gagtcgttcc
     1861 acgcgagggt ggatcctgcg cgaattgcgc aatcgcgcag ttagcgtgct gctacggcag
     1921 gatgatgacc ggatcgtcgg gatcggctcg gtacagcagc agcgtgttgc cgagcacctg
     1981 cgcgacctcg ctgccggttt gccgcgcgat cgcctcggcg gcgtcgtggc ggtcgagcgc
     2041 cgccgcctcg ccgagcttga tcttgaccag ctcgtggtcg gcgagcgcct gatcgaccgc
     2101 ggcgaccagg ccgtcgtcga tcccggcccg gccgatctgc acgatcggct tcagctcgtg
     2161 acccaggctg cgcaggtggc ggcgttgctt gccggtgagc acggccgttt gtacccgaaa
     2221 acgcagaccg cccggcgaat tccgggcggt ctggaaggaa ccctcgggag gaaccttcaa
     2281 actcaggaga tgccacgaac tggaccgtga gcacccggct tgcgccgggc cttcccgagg
     2341 atcgaattcc acgcggcatg ccaaccggcc gggcgcgcga tccgggtcgc gttcaaaagt
     2401 gagatcaggg cgttgccgcg gcggcgcggc ggcttgcaca actcgtgcat ccgcggagtt
     2461 aggccgctac ctgaggcggc gccctggggc cctggagggt cgggccccca ggcggacccc
     2521 ggacgatcag gatccgccga ccagcagctc ggtcgtatcg aacgtgttcg ccatggtgac
     2581 gcccccggac gcgacgatgc cgggctcggt tccttgcttg ggcggcggct cgccgtacgc
     2641 gatcgagtag gccacgggat cgccgtcgcc ggtcgacgcc gtgggggacg acatcatcag
     2701 cgactcgacc tggaacgacc ggcccatggg aagcggaaca caacgatccg catggggagc
     2761 gaccggccgg acgttgagcc cgcggccgtc tccctgcagc acctcctgga ccaccttgcc
     2821 atgccggacc agcgtgaaga tcgacacggt ggtgtggcga tcgacacgat acatctccac
     2881 ggcgaggacg tgatgatcgg gcagcttctc cgccagccgc cgccacgact cccaccgtgc
     2941 ttcacgcacc agatccactg ccttcttgag ttcggacatc gttggttcct ttcacatgga
     3001 cacagtctcg ggacgctcga acacgacgac cccggccacc caggcatcgt cgtgtgaccg
     3061 agcagatgga tgcaagcgct cgtcgcgcaa cgcgaccgcc gggtcggcgc cgcgcagcac
     3121 gcggtcgcgg accgcggcga agaacccgac cgcgccgagg tctccgaccg catcggggct
     3181 ggcgatcacg gcgcgcgctc cggaccgcag gaacgcctcg ggcagaccga tgccgccctc
     3241 gagcgacttg ctgctgtcgg ccgcgtggca cgcgccgagg atcaccagcg gtcgggccgc
     3301 gagctggacg gaggccacat cgtcggcggt gagcgcgtat tgccggtcga gctcgggcga
     3361 cagcaccagg tacgaggact cggacacgtc gttgccgagg atcccgtggg tgtggaactc
     3421 gatgaccgag gcgtcgcgca tcgccagcag cacgcggctg ggcgtcgcgt cggcgccgcg
     3481 caggacccga gcgccgggtt cggtcgccgc ggggtacagc gcgagcggtg gcagcttgag
     3541 gtcgggcagg gtctcggggt tggccaccac gaggcgcggg ctctccgtcg tgccgggcga
     3601 cgacgcgggc gaggcgggcg acgcggggcc ggggagcagg tagctccagg ccatgtcccg
     3661 cggcagcagc cggccgcgcc cgagcaccgg cgcgcgggtc agcacggcga cgcgctcgca
     3721 ggcgcgcagc cggtcgacga ggtcgatggg gatcagcgcc gcggcgtcga cctcgggcga
     3781 cttccaggtc ccgacgtagc gtccggtggt gtcg
//
LOCUS       DASVOR010000097         1545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_263875,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000097 DASVOR010000000
VERSION     DASVOR010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_263875"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VF516_02075"
     CDS             complement(<1..1317)
                     /locus_tag="VF516_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8106479.1"
                     /translation="MRPSTMMDVPLTLDLLADRAERWMGDGEIVSRRPDKSLTRTTYR
                     AVIQRARRLARALVGAGIARGDCVATLMWNHSAHLEAYFGIPLAGAVTHTLNLRLHPN
                     EIAYIANHARDRFVIVDDVLLPLLDKVIAAGGSFERVLVVGNSNDHEPYEAFLDSAAD
                     VALPRLAESDGLATCYTSGTTGMPKGVVYSHRSTLLHTLTAALPDSLDLSRADTLMPV
                     VPMFHVNAWGLPYVAAMTGTRLVFPGPHLDPPSLLDLLAGERVTVAAGVPTLWLGIRE
                     AMDAAPDHWKLVPGLRMIVGGSAAPEQLIRDFDRLGMTLIHAWGMTEMSPLGLVSRVP
                     PQLANADEDTRYRIRAKQGVPPPLVELQIRNEAGDVPRDDKTSGEILVRGPWIAGAYA
                     GGATPERWTDGYFRTGDVARIDEHGFVQLTDRIADLIKSGGEWIASQ"
BASE COUNT          209 a          532 c          576 g          228 t
ORIGIN      
        1 ctgcgacgcg atccactcgc cgccgctctt gatcaggtcc gcgatccggt cggtcagctg
       61 cacgaagccg tgctcgtcga tccgcgcgac gtcgccggtg cggaagtagc cgtcggtcca
      121 ccgctccggc gtggcgccgc cggcgtacgc gcccgcgatc cacgggccgc ggaccaggat
      181 ctcgccgctg gtcttgtcgt cgcgcggcac gtcgccggcc tcgttgcgga tctggagctc
      241 gaccagcggc ggcggcacgc cctgcttggc gcggatccgg tagcgagtgt cctcgtcggc
      301 gttcgccagc tggggtggca cgcgcgacac caggcccagc ggcgacatct cggtcatccc
      361 ccaggcgtgg atcagcgtca tgcccaggcg atcgaagtcg cggatcagct gctcgggggc
      421 ggccgagccg ccgacgatca tgcgcaggcc gggcaccagc ttccagtgat cgggcgcggc
      481 gtccatcgcc tcgcggatcc ccagccagag cgtcgggacg ccggccgcga cggtgacgcg
      541 ctcgccggcc agcaggtcga gcaggctcgg tggatcgagg tgcgggccgg ggaacaccag
      601 ccgggtgccg gtcatcgcgg cgacgtacgg caggccccac gcgttgacgt ggaacatcgg
      661 cacgaccggc atcagcgtgt cggcgcgcga caggtcgagg ctgtcgggca gcgccgcggt
      721 cagggtgtgg agcagcgtcg agcggtggct gtacaccacg cccttgggca tcccggtggt
      781 gccgctggtg tagcaggtcg ccaggccgtc ggactcggcg agccgcggca gcgcgacgtc
      841 ggcggcgctg tcgaggaacg cctcgtaggg ctcgtggtcg ttggagttgc cgaccacgag
      901 cacgcgctcg aacgagccgc cggcggcgat caccttgtcg agcagcggca gcagcacgtc
      961 gtcgacgatc acgaagcggt cgcgcgcatg gttggcgatg taggcgatct cgttggggtg
     1021 cagccgcagg ttgagcgtgt gggtcaccgc gccggccagc gggatcccga agtacgcctc
     1081 gaggtgcgcg ctgtggttcc acatcagggt cgccacgcag tcgccgcgcg cgatcccggc
     1141 gccgaccagc gcgcgcgcca gccgtcgcgc gcgctggatc acggcgcggt acgtggtgcg
     1201 ggtcaggctc ttgtcgggac ggcgcgagac gatctcgcca tcgcccatcc agcgctccgc
     1261 ccggtccgcg agcagatcga gcgtgagtgg aacatccatc atggtgctcg gccgcatcac
     1321 gaaaagatag atccattcgc cagggcgcat gtatgcaaat cttgcggcgc ggccggccgc
     1381 tctccggctc gaccgaccac gacccgggtg ggaggtaacg cgatcgcggc gccgcggggc
     1441 gctgtcgcgg gcggccgtcc gccgtgcgcg gccgaccgtc tgcgtcggtc accggcggtg
     1501 gatcggcccg aggttcattt cgcctggtag ttctcgtaca ccgtc
//
LOCUS       DASVOR010000098         3977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_234635,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000098 DASVOR010000000
VERSION     DASVOR010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3977
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234635"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VF516_02080"
     CDS             <1..1460
                     /locus_tag="VF516_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8106480.1"
                     /translation="PPPPGDVPAPVPGDLPPPASPDDDAAGAMSGDPDAPLHEDSEFG
                     PLIQIERIDITGNTATQTSVIERALPISPGDVLHSSDRRLRETRFKVLALGFFRDVDL
                     AIHKGSQRGQVVIEIHVVERGTLVLNKLWFGTTALSPWWAGADVGDRNLLGHGLAVGG
                     GFIYARHGDLPGTRDQYAGELRAAISSLGGTPWGASGAVTVVRGSDPFCAPPASDPAQ
                     VCAFHYERVGARVRGTSELSALTSLSLGLRVESIDATLPAAPTRALADGTPVAVDLHL
                     EPGTSRVVTLSLGIDHDTRPDPILPHAGSLAALSAELGSTLLAGSYDFATIFGRFDHY
                     WPLGSGHHALAVKLAGGVVVGDAPRFDRIYIADVDRMLTPRALGLVLSTAAPLAILGT
                     RSDKPSYGDLGGSATAEYVFQLFRGSGKQRVYGGDVFVGAGVWGLAERTDLRARDASL
                     FAALPIDLYADAGLRIDTDIGVFELAIANALGRLR"
     gene            1457..2125
                     /locus_tag="VF516_02085"
     CDS             1457..2125
                     /locus_tag="VF516_02085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEX8106481.1"
                     /translation="MRPALAAALRVIVAAAVVLAAVPARADDDKPELQKMRFVERGDD
                     LTVSVSPPGGIGKLFDASNYEALGSGIESTVVIRIQISPQDSDDPVAEQLIRRSVVYD
                     VWDEVYRLQLDERGGQKKFQVKYRAEALKWLTTIEDVPVAQLAVLPLEKVFVLKMVVE
                     LNPVSRETLAEVRRWLSQGTGGGLDRGGAFFGSFVSVFYNPKIADADRVLRIRSQPFY
                     RPRS"
     gene            2122..3612
                     /locus_tag="VF516_02090"
     CDS             2122..3612
                     /locus_tag="VF516_02090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106482.1"
                     /translation="MTRAKRLPLQLKLTAALVLIVMTPLAASAYLIDQLGKAAANVAA
                     SEADARIATMERAMGTYGDLVDTTKRLHGEIAARLALRPDFVQLDPTVNLVKIIDDEP
                     DLRAIALIRPDHTIAAEAERPLPGPTWRDHTLEQPLGTAGATLRLTFAVSATVSEDLG
                     RLKQSIDAARTFSQIRTALPDSYRTAYLILLGVAGLAAAGFGMWASSFLTRRIDALVS
                     TARQVSDGKFDARVELRGADELAELGSAFNTMLDDLDQTRAQVEYLQRMAVWQDVARR
                     LAHEIKNPLTPIQLAVQQCVSSYRGDDARFKQLLVDTGEIVEEEIAGLRRLVDTFRTL
                     GALPKVEAAPIALAEVVEELKLDPTVAAHLTLQPPAQPVTVRADRLLLKRLLANLIEN
                     GIHAGQEAGASGDVVISWAADPARDAATITVDDFGKGVPEASRDRIFEPYVTFKATGT
                     GLGLAIARKIAIEHGGDLVLAPDRAPTGGARFVVSLPLRGPDASLS"
     gene            complement(3652..>3977)
                     /locus_tag="VF516_02095"
     CDS             complement(3652..>3977)
                     /locus_tag="VF516_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106483.1"
                     /translation="WGEAWGTWSADRDPELALPVPAGERVQVTFRWLATAPRGVAQSA
                     RVYLDDQPFDAAIATDGHDHEASFEVTTRRPWLAVRLDIAHPVEAGGRLLGVGLEAAR
                     VHRLP"
BASE COUNT          537 a         1569 c         1339 g          532 t
ORIGIN      
        1 ccccgccacc ccccggggac gtccccgcac cggtccccgg tgacctgccg ccgcccgcca
       61 gccccgacga cgacgccgcg ggcgcgatga gcggcgatcc cgacgcgccc cttcacgagg
      121 acagcgagtt cggcccgctg atccagatcg agcggatcga catcaccggc aacaccgcga
      181 cccagacctc ggtcatcgag cgcgcgctgc cgatcagccc cggcgacgtc ctgcactcca
      241 gcgatcgccg gctgcgcgag acccggttca aggtcctcgc gctcgggttc ttccgcgacg
      301 tcgacctcgc gatccacaag ggcagccagc gcggccaggt cgtgatcgag atccacgtcg
      361 tcgagcgcgg caccctggtg ctcaacaagc tatggttcgg caccaccgcg ctgtcgccgt
      421 ggtgggccgg cgccgacgtg ggcgaccgca acctgctcgg ccacggcctc gcggtcggcg
      481 gcggcttcat ctacgcccgc cacggcgacc tccccggcac gcgcgatcag tacgccggcg
      541 agctgcgcgc ggcgatctcg tcgctcggcg gcacgccctg gggcgccagc ggcgcggtga
      601 cggtggtccg cggcagcgat ccgttctgcg cgccacccgc cagcgatccg gcgcaggtgt
      661 gcgcgttcca ctatgagcgg gtcggcgccc gggtgcgcgg gacctccgag ctcagcgcgc
      721 tgacctcgct gtcgctcggc ctgcgcgtgg agtcgatcga cgccacgctg cccgccgccc
      781 cgacccgcgc gctcgccgat ggcacgccgg tcgcggtcga cctccacctc gagcccggca
      841 ccagccgcgt cgtcaccctc agcctgggca tcgatcacga cacccggccc gatccgatcc
      901 tgccgcacgc cggcagcctg gccgcgctgt cggccgagct cggcagcacg ctgctcgccg
      961 gcagctacga cttcgcgacg atcttcggcc ggttcgatca ttactggccg ctcggcagcg
     1021 gtcaccacgc cctcgcggtc aagctggccg gcggtgtcgt ggtcggcgac gcgccgcggt
     1081 tcgatcggat ctacatcgcc gacgtcgatc gcatgctgac cccgcgcgcg ctcggcctgg
     1141 tgctgtcgac cgcggcgccg ctcgcgatcc tcggcacccg cagcgacaag ccgagctacg
     1201 gcgacctcgg cggcagcgcg accgccgagt acgtgttcca gctgttccgc ggctccggca
     1261 agcagcgggt gtacggcggc gacgtgttcg tcggcgccgg cgtgtggggc ctcgccgagc
     1321 gcaccgacct gcgggcgcgc gacgccagcc tgttcgcggc gctgccgatc gacctctacg
     1381 ccgacgccgg cctgcggatc gacaccgaca tcggcgtgtt cgagctcgcg atcgccaacg
     1441 cgctggggcg gttgcggtga ggcccgcgct cgccgccgcg ctgcgcgtga tcgtcgcggc
     1501 ggcggtcgtg ctggccgccg tcccggcgcg cgccgacgac gacaagcccg agctccagaa
     1561 gatgcggttc gtcgagcgcg gcgacgacct caccgtgtcg gtcagcccgc ccggcgggat
     1621 cggcaagctg ttcgacgcct ccaactacga ggcgctcggc agcggcatcg agtcgaccgt
     1681 cgtgatccgg atccagatct cgccgcagga ctccgacgat ccggtcgccg agcagctgat
     1741 ccggcgctcg gtggtctacg acgtgtggga cgaggtctac cggctgcagc tcgacgagcg
     1801 cggcggccag aagaagttcc aggtcaagta ccgcgccgag gcgctcaagt ggctgaccac
     1861 gatcgaggac gtcccggtcg cccagctcgc cgtgctgccg ctcgagaagg tgttcgtgct
     1921 caagatggtc gtcgagctca acccggtgtc cagggagacc ctcgccgagg tccggcgctg
     1981 gctcagccag ggcaccggcg gcgggctcga ccgcggcggc gcgttcttcg gctcgttcgt
     2041 ctcggtgttc tacaacccca agatcgccga cgccgaccgc gtgctgcgga tccgctcgca
     2101 acccttctac aggccccggt cgtgacccgc gccaagcgct tgcccctcca gctcaagctc
     2161 acggcggccc tcgtgctgat cgtgatgacc ccgctcgcgg cctccgccta cctgatcgac
     2221 cagctcggca aggcggccgc caacgtcgcg gccagcgagg ccgacgcgcg gatcgcgacc
     2281 atggagcgcg cgatgggcac ctacggcgat ctggtcgaca ccaccaagcg cctgcacggc
     2341 gagatcgccg cccggctcgc gctgcgcccc gacttcgtcc agctcgaccc caccgtcaac
     2401 ctggtcaaga tcatcgacga cgagcccgac ctgcgcgcga tcgcgctgat ccgccccgac
     2461 cacacgatcg ccgccgaggc cgagcgtccg ctgcccggac cgacgtggcg cgaccacacc
     2521 ctcgagcagc cgctcggcac cgccggcgcc accctgcgcc tgaccttcgc ggtgtcggcc
     2581 accgtcagcg aggacctcgg ccggctcaag cagtcgatcg acgccgcccg cacgttctcc
     2641 cagatccgca ccgcgctgcc cgacagctac cgcaccgcgt acctgatcct gctcggcgtc
     2701 gccggcctcg ccgccgccgg gttcgggatg tgggcgtcgt cgttcctgac ccggcggatc
     2761 gacgcgctgg tgtcgaccgc gcgccaggtg tccgacggca agttcgacgc ccgggtcgag
     2821 ctgcgcggcg ccgacgagct cgccgagctc ggcagcgcgt tcaacaccat gctcgacgac
     2881 ctcgaccaga cccgggccca ggtcgagtac ctccagcgca tggcggtgtg gcaggacgtc
     2941 gcgcgccggc tcgcccacga gatcaagaac ccgctgaccc cgatccagct cgcggtccag
     3001 cagtgcgtgt cgtcgtaccg gggcgacgac gcccggttca agcagctgct ggtcgacacc
     3061 ggcgagatcg tcgaggagga gatcgccggg ctgcgccggc tggtcgacac cttccgcacg
     3121 ctcggcgcgc tgcccaaggt cgaggccgcc ccgatcgccc tcgccgaggt cgtcgaggag
     3181 ctcaagctcg accccacggt cgccgcccac ctcaccctcc agccgccggc ccagccggtc
     3241 accgtgcgcg ccgaccggct gctgctcaag cggctgctcg ccaacctgat cgagaacggc
     3301 atccacgccg gccaggaagc cggcgcgtcg ggcgacgtcg tgatcagctg ggccgccgac
     3361 cccgcgcgcg acgccgcgac catcaccgtc gacgacttcg gcaagggcgt gcccgaggcg
     3421 agccgcgacc ggatcttcga gccctacgtc accttcaagg ccaccggcac cggcctcggc
     3481 ctcgcgatcg cccgcaagat cgcgatcgag cacggcggcg atctcgtgct cgctccggac
     3541 cgcgccccca ccggcggcgc gcgattcgtg gtctcgttgc cgctgcgtgg cccggacgca
     3601 tccctgtcgt gacctgctcg ccgccctgct cgcgcggtga tcgcgctgcc gtcacggcag
     3661 gcggtggacg cgcgcggcct cgagccccac gccgagcagg cggccgccgg cctcgacggg
     3721 atgcgcgatg tcgagtcgca cggcgagcca cggccggcgg gtcgtcacct cgaacgacgc
     3781 ctcgtggtcg tgaccgtcgg tggcgatcgc cgcgtcgaac ggctggtcat cgaggtagac
     3841 gcgggcgctc tgcgcgacgc cgcgcggcgc ggtggcgagc cagcgaaacg tcacctgcac
     3901 gcgctcaccc gccggcaccg gcagcgccag ctcgggatcg cggtccgccg accaggtgcc
     3961 ccacgcctcg ccccacg
//
LOCUS       DASVOR010000099         2797 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205550,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000099 DASVOR010000000
VERSION     DASVOR010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205550"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..379
                     /locus_tag="VF516_02100"
     CDS             <1..379
                     /locus_tag="VF516_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8106484.1"
                     /translation="LRLSAEALGYLERYRWPGNVRELRNVIERAAVLSHGSAIEPADL
                     PLPITRAAIAADSLSGREDGGARGEPGDPAPDGDRRRIIAALEQCAGNQTRAAKLLGI
                     SRRTLVNRLAAYDIPRPRPRTGK"
     gene            545..1723
                     /locus_tag="VF516_02105"
     CDS             545..1723
                     /locus_tag="VF516_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106485.1"
                     /translation="MAAAAGGSAALLGSWWPRRALAQKAADPALKVEAEVELDALART
                     HPHLHLICTDTLAQLKYSFVVAASGKHPGTGNEFVELSRSFVAGRKPRTKAAYEAGSR
                     ALLDAPPATREAHFGRYAKRAPAAFASAKAEPPPRPSRPATLEDLRSFSTPSSSVIDR
                     PDAESAAKRAHDKDLADGKKWKQVEFYISEVRCIENTGGLFEGTDEIAMGGVGVGATG
                     GTERIKQFHVKDGFSPDKGSNVKKYEHGKRFAVFDVHPEAAFPHEYVVTLAMAELDDG
                     GFGDFLEKLWKKVKGYVVAALAAAGGAIAGALSLSWIPGIGTAIGAIIGAFIGWLVSL
                     FHNDDDIVGHRTRALRLYHATGSYYDKHELTTAKGIPVTMDFKDAGHYRVTGGWRLVA
                     "
     gene            complement(1730..>2797)
                     /locus_tag="VF516_02110"
     CDS             complement(1730..>2797)
                     /locus_tag="VF516_02110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106486.1"
                     /translation="GFQRTVAVKHIKPEYRALKNYIDMFIEEARVGSELAHPNIVQVH
                     DFCSEAGSYYLIMEWVEGVDLGTLIKVYRDAVRDVPWPVAVTIAIGTLRGLGAAHDRV
                     APDGTPAPVIHRDVSPHNVLLGVNGAVKLSDFGLARARDRAASLTAPGTVKGKLSYLA
                     PEVTFGKPNTVQSDLFGVGSVLWETLTGDRLFDGKNDIEIFKKIRACQIPPIQARRKD
                     IPAALAAILELALSADPANRYATADEFAHALDQVIKQAVGINTAAMLAAAVAEARAQL
                     KVADRDADDLPTTEHRTDEHAAVEPPPDPDPELEPEPTVPDGKVNLAGLHRAQRFGAD
                     IPAPVTVPGTRRRMTPQPDED"
BASE COUNT          397 a          962 c         1042 g          396 t
ORIGIN      
        1 gctgcggctg tcggccgagg cgctgggcta cctcgagcgc taccgctggc cgggcaacgt
       61 gcgcgaactg cgcaacgtga tcgagcgcgc cgcggtgctg tcgcatggca gcgcgatcga
      121 gcctgcggat ctcccgctgc cgatcacgcg cgcggcgatc gccgccgact cgctgtccgg
      181 tcgcgaggat ggcggcgccc gcggcgaacc cggcgacccc gcgcccgacg gcgatcgccg
      241 gcggatcatc gcggcgctcg agcagtgcgc cggcaaccag acccgcgccg cgaagctgct
      301 cggcatctcg cggcgcacgc tggtcaatcg cctcgccgcc tacgacatcc cgcgcccgcg
      361 accccgcact ggcaaatgat gccacggcgc tgatcgcccg cgcccggtgc gtcccccggc
      421 acgcggcgtg catttcgtcg cgcgcaacac cccttgcgta tagggaacac cagggaaggg
      481 acagaccgat atgacgtcat ccagagatcg agatcgtgca gcgtggaccc ggcgacagtt
      541 cgcgatggcg gccgcggccg gaggcagtgc ggcgctcctg ggctcgtggt ggccgcggcg
      601 tgcgctcgcg cagaaggcgg ccgacccggc gctcaaggtc gaggccgagg tcgagctcga
      661 cgcgctcgcc aggacgcacc cccacctcca cctgatctgc accgacacgc tggcccagct
      721 caagtactcg ttcgtcgtcg ctgcatcggg gaagcacccc gggaccggaa acgagttcgt
      781 cgagctgtcg cggtcgttcg tcgccggccg caagccgagg accaaggcgg cctacgaggc
      841 cgggtcccgc gcgctgctcg acgcgccgcc ggcgacccgc gaggcgcact tcggccgcta
      901 cgccaagcgc gcgccggcgg cgttcgcgtc ggccaaggcc gagccgccgc ccaggccgtc
      961 caggcccgcg acgctggagg acctgcggtc gttcagcacg ccgtcgtcgt cggtgatcga
     1021 ccggcccgac gccgagtccg ccgcgaagcg cgcccacgac aaggacctcg cggacggcaa
     1081 gaagtggaag caggtcgagt tctacatcag cgaggtccgg tgcatcgaga acaccggcgg
     1141 cctgttcgag ggcaccgacg agatcgccat gggcggggtc ggcgtcggcg cgacgggcgg
     1201 caccgagcgg atcaagcagt tccacgtcaa ggacggcttc tcgccggaca agggctcgaa
     1261 cgtcaagaag tacgagcacg gcaagcggtt cgccgtgttc gatgtccacc ccgaggcggc
     1321 gtttccccac gagtacgtcg tcaccctcgc gatggccgag ctcgacgacg gcggcttcgg
     1381 cgacttcctc gagaagctgt ggaagaaggt caagggctac gtcgtggccg cgctcgccgc
     1441 cgcgggcggc gcgatcgccg gcgccctgag cctgtcgtgg atcccgggca tcggcaccgc
     1501 gatcggcgcg atcatcggcg cgttcatcgg ctggctggtg tcgctgttcc acaacgacga
     1561 cgacatcgtc ggccaccgga cccgggcgct gcgcctctac cacgcgacgg ggtcgtacta
     1621 cgacaagcac gagctcacga cggccaaggg catcccggtc accatggact tcaaggacgc
     1681 cggccactac cgcgtcaccg gcggctggcg gctcgtggcg tagcccgggt cagtcctcgt
     1741 cgggctgcgg cgtcatccgc cggcgcgtgc cggggacggt gaccggggcg gggatgtcgg
     1801 cgccgaaccg ctgcgcccgg tgcaggccgg cgaggttgac cttgccgtcc ggcacggtcg
     1861 gctcgggctc cagctccggg tcgggatccg gcggtggctc gacggcggcg tgctcgtcgg
     1921 tgcggtgctc ggtggtcggc aggtcgtcgg cgtcgcgatc ggcgaccttg agctgggccc
     1981 gggcctcggc gaccgcggcg gccagcatcg cggcggtgtt gatcccgacc gcctgcttga
     2041 tgacctggtc gagcgcgtgg gcgaactcgt cggcggtggc gtagcggttg gcggggtcgg
     2101 ccgacagcgc gagctcgagg atcgcggcga gcgcggcggg gatgtccttg cggcgggcct
     2161 ggatcggcgg gatctggcag gcgcggatct tcttgaagat ctcgatgtcg ttcttgccgt
     2221 cgaacagccg gtcgccggtc agggtctccc acagcacgct gccgacgccg aacaggtcgg
     2281 actggacggt gttgggcttg ccgaaggtga cctcgggggc gaggtaggac agcttgccct
     2341 tgacggtgcc gggagcggtg aggctcgccg cgcgatcgcg cgcgcgggcg aggccgaagt
     2401 cggacagctt gaccgcgccg ttgacgccga gcaggacgtt gtgcggcgac acgtcgcggt
     2461 ggatcaccgg cgccggggtg ccgtcggggg cgacccggtc gtgcgcggca cccaggccgc
     2521 gcagggtgcc gatcgcgatc gtgacggcga ccggccaggg cacgtcgcgg accgcgtcgc
     2581 ggtagacctt gatcagggta ccgaggtcga cgccctcgac ccactccatg atcaggtagt
     2641 aggagccggc ctcgctgcag aagtcgtgga cctggacgat gttggggtgg gcgagctcgc
     2701 tgccgacgcg cgcctcctcg atgaacatgt cgatgtagtt cttgagcgcg cggtactcgg
     2761 gcttgatgtg cttgaccgcg accgtgcgct ggaaccc
//
LOCUS       DASVOR010000100         3889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_176327,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000100 DASVOR010000000
VERSION     DASVOR010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176327"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1995)
                     /locus_tag="VF516_02115"
     CDS             complement(172..1995)
                     /locus_tag="VF516_02115"
                     /inference="COORDINATES: protein motif:HMM:NF025316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX8106487.1"
                     /translation="MRTCALIVVLAATFVAGCGDSTMPATTMADICSNGAAVEGDSCG
                     AGMICRSSRCVASACGDGIVAAGEECDDGNKVDGDGCDSNCKFTCLTSDPSRNCTSAD
                     ACAGKGVCGDAHVCIAGTPLADGHACGNGGSNVCHAGVCMPPHCGDGEVDPGEQCDGG
                     PGCKANCTFLCTNDPATQCGGTAAACEKFACKADHSCELVADLAANFQQCDQNPANVC
                     ILGTCKAAPVCGNGIVEFGEDCDDGNFRSNDGCDRCSFEQAARITSLVQQFGTDAFCT
                     KNALGTAISRLQAAQDFIQQTWSFPVGDGTISLVFKFMGTIDPSGARSTFNLGFFDAT
                     PVRFNQQDDGTFADNYNGTNDLDWWYTLRQQDPNNPFDTPNLSLDANGTPRVQLPAEI
                     FDRHLTAGPGTIENIRLLFALAPANVALFNVHIDATLDTKLSKPTVSTTNTPPGHLAS
                     EHLSPDFITFESSGISSALGGMCSDVSAKSLANALPGLLGLCADPADATGGTPAFQVD
                     NHLLDVFIFGCQLFTTDPTTGKPGFVPTIAPTQPDGSLDGSTYTFALDPATHKVTSCT
                     KDGNDAVLDVCLANALFSSYFKIAADRVILRTDKPSVLPPP"
     gene            2438..3001
                     /locus_tag="VF516_02120"
     CDS             2438..3001
                     /locus_tag="VF516_02120"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8106488.1"
                     /translation="MAQTVAKRGFAATAVTEVCRAAKVSTRAYYEHFADKEECFMATF
                     DCGVGLLQGRVAKAYAQPASWPVRMHRGLEELLHLLAAEPAFANLAIVEMLAAGPRGR
                     ERVRKLLQNFQRFFDDAPRQPGQPRVPPVAVEAVVAGVFGLLFNYVSTKRTTDLPDLL
                     PEITYFVLVPFIGPRAAAEAAGLPLAE"
     gene            3055..>3889
                     /locus_tag="VF516_02125"
     CDS             3055..>3889
                     /locus_tag="VF516_02125"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106489.1"
                     /translation="MHQVPRSLVAVTAVVGVLLGSWAVRAPRVAFAQPAPASSPGAPA
                     PGEDEALYSCKQRTGQVAVTFKPETELKDLITWVMGFTCKNFIFEPRIVSTGKKVTLI
                     VPNKMTPTEAYRLFLVALSTMGLTVVPKGNALRVVEAPTARSDTVPIFTRGIPGDEDR
                     IVRYVLRPTYTPVETLRQALDSIRSPSGLVLAVGTMIIINDYASQVRDMLSLARAIDV
                     PGGTDGIYTIPVHHADATQLAQKLNDILGISAAAAANPAGGRRAPNMPPQAPGMPPPP
                     NN"
BASE COUNT          663 a         1257 c         1314 g          655 t
ORIGIN      
        1 accgaggtcg aactacatgc gcaccgatgg aacggcatgc ccggcacctg cgaggtggtg
       61 ctcgagcgtg cgtacgagcg tgttgcaggg ctagcgatgt ggtgtgccga ataccggcat
      121 cagatcgatg tcagcgcccg ctgcaaatcc tgcggctcga aggcccgacc ttcaaggagg
      181 aggcagcacc gatggtttgt ccgtccggag gatcacgcgg tcggccgcga tcttgaagta
      241 gctggagaag agagcgttgg cgaggcagac atcgagaaca gcgtcgttac cgtctttcgt
      301 acagctcgtc accttgtggg tcgcggggtc gagcgcgaag gtgtaagtcg atccgtcgag
      361 cgaaccatcg ggctgcgtcg gtgcaatcgt gggcacgaac ccaggcttgc cggtggtcgg
      421 atccgtggtg aagagctgac acccgaagat gaacacatcg agcagatggt tatcgacctg
      481 gaacgcgggc gtacccccgg tcgcatcggc tggatcggcg cacaggccga gcaggccggg
      541 caaggcgttg gccagcgact tggcgctcac gtccgagcac atgccaccca gggcggagga
      601 gataccggac gactcgaagg tgatgaagtc cggagacagg tgctcgctcg cgagatgacc
      661 cgggggcgtg ttcgtcgtgg acaccgtggg tttggacagc ttggtatcca gggtcgcatc
      721 gatatgcacg ttgaacagcg cgacgttcgc cggtgcgagg gcgaacagca gcctgatgtt
      781 ctcgatcgtg cccggtccgg cggtcaggtg acggtcgaag atttcagcag gaagctggac
      841 gcgcggcgtt ccattggcgt cgagcgagag attgggggtg tcgaacgggt tgttcgggtc
      901 ttgctggcgc agggtgtacc accagtcgag atcgttcgtg ccgttgtagt tgtcggcgaa
      961 cgtgccgtca tcctgctggt tgaacctgac cggggtcgcg tcgaaaaacc ccagattgaa
     1021 tgtcgacctg gctccggacg gatcgatggt ccccatgaac ttgaacacga gcgaaatggt
     1081 cccgtccccg accggaaagc tccaggtctg ctgaatgaag tcctgggcag cctggagcct
     1141 cgagatcgcc gtgccgagcg cgttcttggt acagaacgcg tcggtgccga actgctgcac
     1201 gagcgacgtg atgcgggcag cctgctcgaa cgagcaccgg tcgcagccgt cgttgctgcg
     1261 gaagttgccg tcgtcgcagt cttccccgaa ctccacgatc ccgttgccgc acaccggcgc
     1321 cgccttgcac gtaccgagga tgcagacgtt cgccgggttt tgatcgcact gctggaagtt
     1381 tgccgcgagg tcggcgacga gctcgcagct gtggtccgcc ttgcacgcaa acttctcgca
     1441 ggcggccgcc gtcccgccgc actgggtagc ggggtcgttg gtgcacagga acgtgcagtt
     1501 tgccttgcag ccaggcccac cgtcgcactg ttcgccgggg tcgacctcgc cgtctccgca
     1561 gtgcggcggc atgcagacgc ctgcgtggca gacgttgctc cccccgttcc cgcaggcatg
     1621 gccatcggcc agcggcgtgc cggcgatgca gacgtgggca tcgccgcaaa cacccttgcc
     1681 ggcgcacgca tcggccgacg tgcagttgcg cgaaggatcc gatgtcaggc aggtgaactt
     1741 gcagttcgag tcgcagccat cgccgtcgac cttgttgccg tcgtcgcatt cctcaccggc
     1801 ggcgacgatg ccatcgccgc acgcggaggc gacgcaccgg ctcgaccggc agatcatgcc
     1861 agcgccgcag ctatcgccct ccacggcggc gccgttcgag cagatatcgg ccatcgtcgt
     1921 ggccggcatc gtgctgtctc cacatcctgc gacgaaggta gccgcgagta ccacaatgag
     1981 cgcgcatgtg cgcatgagag ggcctcctaa tctatcgaga cgtctctacg cttccttcgt
     2041 accggctgtc cagagaaaag cgggtgtttc tggatctggg aaaagtttcg tcacatcgct
     2101 agcatgaagg agcgcgtgga gttgtgagag gctctcgtgg aagatgatca ggaaatggcc
     2161 cccccgacat cggcgcaggt cgctgcgtct tcgaaggaag atcttgtcac gtagaatgcg
     2221 aacgcctacc cccggatatc gacgacttcg gcgcgcgggt cgggcaagct cttccgcccg
     2281 tgggaggatg aggaaacctg acccccgaga ccgacccgtc gagggcttcc cggggtctcg
     2341 gtcagcgacg gatcccgagc gcaagctgcc gcggggccgc cataagcacc cgcgcaagtt
     2401 catcgcgcat acccagcgcg atcgcctcat cgatgccatg gcacagacgg tggcgaagcg
     2461 gggctttgcc gcgactgcgg tgaccgaggt ctgcagagcc gccaaggtat cgacccgggc
     2521 gtattacgag cacttcgccg acaaggagga gtgcttcatg gcgacgttcg attgcggcgt
     2581 cggcttgctg cagggcaggg tggccaaggc gtacgcgcaa ccggcgagct ggccggtgcg
     2641 gatgcaccgc ggcctcgagg agttgctcca cctgctggcc gccgaacctg cgttcgcgaa
     2701 cctcgccatc gtggagatgc tcgccgccgg gccgcgcggg cgcgagcgcg tccgcaagct
     2761 gctccagaat ttccagcggt tcttcgacga cgcgccccga cagcccgggc agccgcgcgt
     2821 gccgccggtc gcggtggaag cggtcgtcgc cggtgtgttc ggcctgctgt tcaactacgt
     2881 gtcgaccaag cggaccaccg acctgcccga cctcttgccc gagatcacgt acttcgtgct
     2941 cgtgccattc atcggcccgc gtgccgctgc cgaggcagcc gggctgccgc tggcggagtg
     3001 agtgcgccgc ggccgacgac cgctgccgct tcgaagtcct cttcaggaaa gaccatgcat
     3061 caggtacctc gttcgctcgt cgcggtcacc gccgtcgtcg gcgtcctcct gggaagctgg
     3121 gcagtccggg cgccgcgggt cgcgttcgcg caaccggcgc cggcgtcctc cccgggcgcg
     3181 cccgcgcccg gggaggacga ggcactgtac agctgcaagc agcgcacggg gcaggtcgcg
     3241 gtgacgttca agccggagac cgagctcaag gatctcatca cctgggtgat gggcttcacg
     3301 tgcaagaact tcatcttcga gccgcgcatc gtgtcgaccg gcaagaaggt cacgctgatc
     3361 gtgcccaaca agatgacccc gaccgaggcc taccggctgt tcctggtcgc gctgtcgacg
     3421 atggggctca cggtggtgcc caagggcaac gcgctccgcg tcgtcgaggc tccgaccgcg
     3481 aggagcgaca cggtgccgat cttcacgcgc ggcatcccag gcgacgagga tcggatcgtg
     3541 cgctacgtgc tgcgaccgac ctacacgccg gtcgagacgc tgcgccaggc gctcgattcg
     3601 atccgctcgc cgtctggcct cgtgctggcc gtcggcacga tgatcatcat caacgactat
     3661 gccagccagg tccgcgacat gctgtcgctc gcgcgagcga tcgacgtgcc cggcggcacc
     3721 gacggcatct acacgatccc ggtccaccac gccgacgcga cccagctcgc gcagaagctc
     3781 aacgacatcc tcgggatctc ggccgcggcg gccgcgaacc cggcgggcgg tcggcgggcg
     3841 cccaacatgc cgccgcaggc gcccgggatg ccgcctccgc cgaacaacc
//
LOCUS       DASVOR010000101         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_147120,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000101 DASVOR010000000
VERSION     DASVOR010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147120"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..671)
                     /locus_tag="VF516_02130"
     CDS             complement(<1..671)
                     /locus_tag="VF516_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106490.1"
                     /translation="MIAGMLLAARAHEAAHELPFSSVDRILAATKASRSRAYEIKQAI
                     EARMQELERPPGRPRAEPAPAPCDARAALTYEALCFLMAHPGCLEVGEQRARYSDVYR
                     HFVLGLRERHADVTLAELAGAIALPRGTLEDWLRSPLSAELGSAGEAAPCDASATEDV
                     PDPGREAPRDARQAQIETVLAAWRAWHGSFGAFCAHVRCDHRLELGTTLIANILFEYG
                     LRVPAR"
     gene            complement(748..1281)
                     /locus_tag="VF516_02135"
     CDS             complement(748..1281)
                     /locus_tag="VF516_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106491.1"
                     /translation="MPNSAPSLPPTPDRTAGLLLVARRFAEEYGLPRSSVEQILAATG
                     ASRESALEIEAALVAALEELAQSMSPCDRAPEVDDRGRIDALTHEALCYVMQNPACTR
                     RTARGRHAAVYRQFVIELRARYADVSAPAFADAVHVPVSTLDAWMRSRRGVVALVAQM
                     PIAAQRASAAQTPTSPP"
     gene            complement(1345..>1616)
                     /locus_tag="VF516_02140"
     CDS             complement(1345..>1616)
                     /locus_tag="VF516_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106492.1"
                     /translation="AEMKRRLAAHLRWYNSARTHHALGGLLVPADRYYGRAEEVLARI
                     ESGGQRDGDELELRDRCLELFKVVSKNGTPEVWLLGQRLTVPALP"
BASE COUNT          205 a          596 c          583 g          232 t
ORIGIN      
        1 cgcgccggga cgcgcagccc gtactcgaac aggatgttcg cgatgagcgt ggtgccgagc
       61 tcgaggcgat ggtcgcagcg cacgtgcgcg cagaacgcgc cgaagctgcc atgccacgcg
      121 cgccaggcgg cgagcacggt ctcgatctgc gcctgccggg cgtcgcgcgg cgcttcgcgt
      181 cccggatcgg gaacatcctc ggtcgcgctc gcatcgcatg gcgcggcctc gccggcgctg
      241 ccgagctcgg cgctgagcgg cgaccgcagc cagtcctcca gcgtgccgcg cggcagcgcg
      301 atcgcgccgg cgagttcggc gagcgtcacg tcggcgtggc gctcacgcag cccgagcacg
      361 aagtgccggt agacgtccga gtaccgcgca cgctgctccc cgacctccaa gcatcccggg
      421 tgtgccatga ggaagcacag tgcctcgtag gtcagcgcgg cgcgtgcatc gcacggtgcg
      481 ggagcgggct cggcgcgcgg tcggcccggc ggtcgctcga gctcctgcat ccgcgcctcg
      541 atcgcttgct tgatctcgta ggcccgcgag cggctcgcct tggtcgcggc aaggatccgg
      601 tccacgctcg agaacggcag ctcgtgcgcc gcctcgtggg cccgggcggc cagcagcata
      661 ccggcgatca cggtcggcct cgttgcgggg gatggatccg ggatcgacaa ggcggtctcg
      721 cttgcgctgc gccgggagcg cgctgttcta cggggggctc gtcggggtct gcgccgcgct
      781 cgcccgctgt gccgcgatcg gcatctgcgc caccagcgcc accacgccgc gccggctgcg
      841 catccaggcg tccagcgtgc tgaccggcac gtgcaccgca tcggcgaacg cgggcgcgga
      901 cacgtcggcg tatcgcgccc gcagctcgat cacgaactgc cggtagaccg ccgcgtggcg
      961 cccgcgtgcg gtgcgccgcg tgcaggcggg gttctgcatc acgtagcaca gcgcctcgtg
     1021 ggtcagcgcg tcgatccggc cacggtcgtc gacctccggc gcgcggtcgc acgggctcat
     1081 cgactgcgcc agctcctcga gtgctgcgac cagcgccgcc tcgatctcga gggcggactc
     1141 gcggctggcc cccgtcgccg cgaggatctg ctcgacgctc gaccgcggca ggccgtactc
     1201 ctcggcgaat cggcgcgcga ccaggagcaa gccggcggtc cggtccggcg tgggtgggag
     1261 ggatggcgca gagttcggca cggcgggtcc ttggtggggg cgacctcggt tttgccccag
     1321 acctctgaca acgaccgcgc cgcgctacgg gagtgccggc accgtcaagc gctgtccgag
     1381 cagccagacc tccggagtgc cgttcttgct cacgaccttg aacagctcca ggcaccggtc
     1441 gcggagctcg agctcgtcgc cgtcccgctg ccctccggat tcgatgcgcg ccagcacctc
     1501 ctcggcccgg ccgtagtacc gatctgctgg cacgagcagc ccacccagcg cgtgatgggt
     1561 ccgcgcactg ttgtaccagc gcaggtgggc cgccagccgc cgcttcatct cggcca
//
LOCUS       DASVOR010000102         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_380846,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000102 DASVOR010000000
VERSION     DASVOR010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380846"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF516_02145"
     CDS             complement(<1..304)
                     /locus_tag="VF516_02145"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008671 - 2-dehydro-3-deoxygalactonokinase
                     activity [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEX8106493.1"
                     /translation="MTQLIGLDWGTTSLRAYRFDGSGQVIESRHRPSGILQIAAERGA
                     DGFERALDEVCGDWIAADPGCALLACGMIGSAQGWREAPYVEVTASLDALGGALTAI"
     gene            491..1588
                     /locus_tag="VF516_02150"
     CDS             491..1588
                     /locus_tag="VF516_02150"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX8106494.1"
                     /translation="MLCACGRGGGTAGGPDTVQGPGADPSCFRPWGKTTPYYQWKKKP
                     GPYRIALDNGFIGNTWRIQMIKTAKAYADQPAVKAKLKEFKVISTGEDLPAQIAAANN
                     LIDAGYDAVIVNALNPTAFAPVVTKAKKAGVVLLSFDNVIESPDNIVINVDQVELGRL
                     AAKWLLKEVQADGGKLLEVRGPSGNSVDRDRHDGFREVLGKSGKKFEVIEVVGKWDDG
                     TAQKATADAIAIHKKFDGIYVQGGSTGTVRALLDAHHPMVPISGETENGFRKLCAEHA
                     AEGLHCSSAGTGPAQVAVTIKAAIAALEGEKVPQSISLPTSYTEYPNIKEGTDFYPAL
                     SDNFFVGNSFPDCNIGFTADEIMGKSEANSQ"
     gene            1596..>2105
                     /locus_tag="VF516_02155"
     CDS             1596..>2105
                     /locus_tag="VF516_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8106495.1"
                     /translation="MTAADRPAADLHPPLLRMEGISKHSGGVRALDGARLACARGRIH
                     AVLGENGAGKSTLIKVLSGVVRPDAGTIELDGAPVELRRPADAQRLGIVSVFQELSLL
                     PDLTVADNIGIVDPPRRWGLIDARAQRRRAEAALARLGVRDIHPHARASALAISTIWR
                     RDSGRSRTGA"
BASE COUNT          347 a          742 c          723 g          293 t
ORIGIN      
        1 cgatcgcggt cagcgcgccg ccgagcgcgt cgaggctcgc cgtgacctcg acgtacggag
       61 cctcgcgcca gccctgcgcg ctgccgatca tgccgcacgc cagcagcgcg caccccgggt
      121 cggcggcgat ccagtccccg cacacctcgt ccagcgcgcg ctcgaagccg tccgcgccgc
      181 gctccgcggc gatctgcagg atgcccgacg gccgatgccg cgactcgatg acctgtccac
      241 tcccgtcgaa ccgataggcg cggagcgagg tcgtgcccca gtcgaggccg atgagctgcg
      301 tcattgccac ggcgacccta ggggagctac cggaaaaact ccagggccga ttgacatgcg
      361 tgatttcgcg gtcctacgct gcctgtcact cgtcgagcga aaggcaatcg caatgaagct
      421 acacgatctg cgttgcgtgg gtctgcgtcg catggtccgg ccggccattg caacgatcgg
      481 tgttggcctc atgctgtgtg cgtgcggtcg cggcggggga accgccggcg ggcccgacac
      541 cgtgcagggg cccggcgccg atccgtcgtg cttccggccg tggggaaaga ccacacccta
      601 ctatcagtgg aagaagaagc cgggcccgta ccgcatcgcg ctggacaatg gcttcatcgg
      661 caacacctgg cggatccaga tgatcaagac cgccaaggcg tatgccgatc agcccgcggt
      721 caaggcgaag ctgaaggagt tcaaggtgat ctcgaccggc gaggacctgc cggcgcagat
      781 cgccgccgcc aacaacctga tcgatgccgg ctacgacgcc gtgatcgtca acgcgctcaa
      841 cccgacggcg ttcgcgccgg tggtcacgaa ggccaagaag gcgggagtcg tcctgttgtc
      901 gttcgacaac gtcatcgagt cccccgacaa catcgtgatc aacgtcgatc aggtcgagct
      961 cggccggctc gcggcgaagt ggctgctcaa ggaggtccag gccgacggcg gcaagctgct
     1021 cgaggtccgc ggcccgtccg gcaactcggt cgatcgcgac cgccacgacg gcttccgcga
     1081 ggtgctcggc aagtccggaa agaagttcga ggtcatcgag gtcgtcggca agtgggacga
     1141 cggcaccgcg cagaaggcca ccgccgacgc gatcgccatc cacaagaagt tcgacgggat
     1201 ctacgtccag ggcggctcga ccggcacggt gcgcgcgctg ctcgacgcgc accacccgat
     1261 ggtcccgatc tccggcgaga ccgagaacgg gttccgcaag ctgtgcgccg agcacgccgc
     1321 cgaggggctg cactgctcgt cggccggcac cgggccggcc caggtcgcgg tgacgatcaa
     1381 ggcggcgatc gccgcgctcg agggcgagaa ggtcccgcag tcgatctcgc tgccgacctc
     1441 gtacaccgag tatcccaaca tcaaggaagg caccgacttc tatcccgcgc tgtcggacaa
     1501 cttcttcgtc ggcaactcgt tcccggactg caacatcggg ttcacggccg acgagatcat
     1561 gggcaagtcg gaggccaatt cgcagtagct gcgccgtgac cgccgccgat cggccggccg
     1621 ccgacctcca ccctccgctg ctccgcatgg agggaatctc gaagcactcc ggcggggtcc
     1681 gggcgctcga cggcgctcgc ctggcctgcg cgcgcgggcg gatccacgcc gtgctcggcg
     1741 agaacggcgc cggcaagtcg acgctgatca aggtgctgtc gggcgtggtc cgccccgacg
     1801 cggggacgat cgagctcgac ggtgcgccgg tcgagctgcg ccggcccgcc gacgcgcagc
     1861 ggctcgggat cgtgagcgtg ttccaggagc tgtcgctgct ccccgacctc acggtggccg
     1921 acaacatcgg catcgtcgac ccaccgcggc ggtggggcct gatcgacgcg cgcgcccagc
     1981 gccggcgcgc cgaggcggcc ctggcgcggc tcggggtccg cgacatccac ccgcacgcgc
     2041 gggccagcgc cttggcgatc tcgacgatct ggcggcgcga cagcggcagg tcgcggaccg
     2101 gcgcg
//
LOCUS       DASVOR010000103         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_59420,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000103 DASVOR010000000
VERSION     DASVOR010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59420"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..852)
                     /locus_tag="VF516_02160"
     CDS             complement(22..852)
                     /locus_tag="VF516_02160"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8106496.1"
                     /translation="MSAPLREAASVILLRRAEAGTEPGFEVFLLRRHRQAKFMASAFV
                     FPGGAAEPGEDARTAAARELFEEAGVLLAHADARLEAQTLEMPIQAQLRRRILDGADA
                     ATAMVAAGLAWSTDVLVPWAHWITPSVEPRRFSARFFTCELPSAQVPQFDEVETVDQV
                     WVRPREALERAGELRLPPPQIRTMWELSQLGSVEEVLAAGRARSEEPHPIMPRLRTPA
                     PGDPVRLLLPWDPEYTEVGSGDALPLTYTPRWAVGPTRFVMEGQTWKYVAAPGSTTAG
                     "
     gene            complement(944..1408)
                     /locus_tag="VF516_02165"
     CDS             complement(944..1408)
                     /locus_tag="VF516_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106497.1"
                     /translation="MRARCSRRSACCFPSAPPAHVSSEVVSCDPRLRSPYRGTETGRA
                     VLRLRVQIAPRRVAPYEVVLEVDDLAIEVHEDHGGFVVGAGAIDALFVVECQPRHGFL
                     TWFGVFARCRGLADRLLLETPESAVARFVERAIEDRLGLDDEPVRGELRLGL"
     gene            complement(1431..2363)
                     /locus_tag="VF516_02170"
     CDS             complement(1431..2363)
                     /locus_tag="VF516_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8106498.1"
                     /translation="MNDRPRFALRFISGKYQGGEFPLRMNREIIIGRSSDLDMVLVED
                     MVSRRHAKISSTEAEVYIQDMGSTNGTFVNGEKIAGRALLHEGDRILVGTSIIKVVAV
                     EGQVANQSEAEARRRLEAGAQQRQATQGRPMSGVIEEIPLPDLLQLLSTSRKSGVLTI
                     NNGVSMGKIYLRKGALYFATINDDFAVSPQKSIYRMLTWATGTFELEPSVELQVMEEV
                     QESTEALLMEGVRQLDEFRNLQKQLPPLGSPLAVPTPLAGKLRELTPSELDTFQLVLD
                     HGQLQKVLDNFPGTDLDAAQNLIALMKREFVVVP"
     gene            complement(2456..>2659)
                     /locus_tag="VF516_02175"
     CDS             complement(2456..>2659)
                     /locus_tag="VF516_02175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106499.1"
                     /translation="PGVEVHTIAAARDWVVPLPTTRLAGATATTVPLGHSSLVVSEEV
                     YRRVVLALRPPHEPASDETPATD"
BASE COUNT          399 a          974 c          911 g          375 t
ORIGIN      
        1 cgccccccgg gcccgcgatg actagcccgc ggtcgtcgaa ccaggtgcgg cgacgtactt
       61 ccacgtctgc ccctccatca cgaagcgggt cggcccgacc gcccagcgcg gcgtgtaggt
      121 cagcggcagc gcatcgccgc tgccgacctc ggtgtactcg gggtcccacg gcagcagcag
      181 gcgcaccgga tcgcccggcg ccggcgtgcg cagccgtggc atgatcgggt gcggctcctc
      241 gctgcgcgcc cggccggcgg cgagcacctc ctcgaccgag ccgagctgcg acagctccca
      301 catcgtgcgg atctgaggcg gcggcagccg cagctcgccg gcgcgctcca gcgcctcgcg
      361 gggccgcacc cagacctggt ccacggtctc gacctcgtcg aactgcggga cctgagcgct
      421 cggcagctcg caggtgaaga accgcgccga gaaccgccgc ggctcgaccg acggcgtgat
      481 ccagtgcgcc cacggcacca gcacgtcggt cgaccacgcc agcccggcgg cgaccatcgc
      541 ggtcgcggca tcggcgccgt cgaggatgcg gcggcgcagc tgggcctgga tcggcatctc
      601 cagggtctgg gcctcgagcc gggcgtcggc gtgcgcgagc agcacgccgg cctcctcgaa
      661 cagctcgcgc gccgccgcgg tccgcgcgtc ctcgccgggc tcggcggcgc cgccggggaa
      721 cacgaacgcc gacgccatga acttggcctg gcggtggcgc cggagcagga acacctcgaa
      781 tcccggctcg gttcccgcct cggcgcgccg cagcaggatc accgacgcgg cctcgcgcag
      841 cggcgcgctc atcgcaggct cccggccagg ctccgggcca ggctcccggc tcgatcgcag
      901 gacacggcca tggcgccgtt ggtaccacga cgacggcgcc cgatcacagc cccaggcgta
      961 gctcgccgcg caccggctcg tcgtccagcc ccagccggtc ctcgatcgcg cgctcgacga
     1021 accgggccac cgcgctctcc ggggtctcga gcagcagccg gtcggccagc ccgcggcagc
     1081 gcgcgaacac gccgaaccag gtcaggaagc cgtgacgggg ctgacactcg accacgaaca
     1141 gcgcgtcgat cgcgccggcg ccgaccacga acccgccatg gtcctcatgc acctcgatcg
     1201 cgaggtcgtc gacctccagc acgacctcgt acggcgcgac cctgcgcggg gcgatctgca
     1261 cccgcagccg gagcaccgcg cgcccggtct cggtcccgcg atacgggctg cgcaggcgcg
     1321 gatcgcagct caccacctcc gacgagacgt gggccggagg cgccgagggg aagcaacacg
     1381 ccgatcgccg cgagcagcga gcccgcatgt cgtgatcata ccgctgcctg ctacgggacg
     1441 acgacgaatt ctcgcttcat cagcgcgatc aggttctgcg ccgcgtcgag gtcggtgccc
     1501 ggaaagttgt cgagcacctt ctggagctgg ccgtggtcga gcacgagctg gaaggtgtcg
     1561 agctccgacg gcgtcagctc gcgcagcttg ccggccagcg gggtcggcac ggcgagcggc
     1621 gagcccagcg gcggcagctg cttctgcagg ttgcggaact cgtcgagctg ccgcacgccc
     1681 tccatcagca gcgcctcggt cgactcctgg acctcctcca tcacctggag ctcgacgctg
     1741 ggctcgagct cgaacgtgcc ggtcgcccag gtcagcatgc ggtagatcga cttctgcggg
     1801 ctgaccgcga agtcgtcgtt gatcgtcgcg aagtacagcg cgcccttgcg caggtagatc
     1861 ttgcccatgc tcaccccgtt gttgatggtg agcacaccgc tcttgcggct ggtcgacagc
     1921 agctgcagca ggtcgggcag cgggatctcc tcgatcacgc ccgacatcgg acggccctgg
     1981 gtcgcctggc gctgctgcgc cccggcctcg aggcggcggc gcgcctcggc ctcgctctgg
     2041 ttggcgacct ggccctcgac cgcgacgacc ttgatgatcg aggtgccgac caggatgcga
     2101 tcgccctcgt ggagcagcgc gcgcccggcg atcttctcgc cgttgacgaa cgtgccgttg
     2161 gtcgagccca tgtcctggat gtagacctcg gcctccgtcg agctgatctt cgcgtggcgg
     2221 cgcgagacca tgtcctcgac cagcaccatg tcgagatcgc tcgaacgacc gatgatgatc
     2281 tcccggttca tgcgaagcgg gaactcgcct ccctggtact tgcccgagat gaagcggagc
     2341 gcgaagcgag gacgatcgtt catggatttg gcgctggtaa ccatgggtct ctgaccagct
     2401 gcaacaactc gaaaattcta gttctcaaga tgccacacgc gcaagcaaac gggggtcaat
     2461 cggtggcggg cgtttcgtcg cttgccggct cgtgcggcgg acggagcgca agcacgacac
     2521 ggcggtagac ttcttccgac accaccaggc tggagtgacc caggggcacg gtggtcgccg
     2581 tcgccccggc gagccgtgtc gtcggcagcg gaacgaccca gtcgcgggcg gcggcgatcg
     2641 tgtggacctc gactcccgg
//
LOCUS       DASVOR010000104         4397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_497888,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000104 DASVOR010000000
VERSION     DASVOR010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4397
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_497888"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /locus_tag="VF516_02180"
     CDS             <1..429
                     /locus_tag="VF516_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX8106500.1"
                     /translation="VGKRIKERDPEAKVVLADPVGSSLADWVNTGTLGPDGPYTVEGI
                     GSGSVPGNLHRDALDYAEKVPDAEAYAMVERLVKEEGLTVGGSTGVNVVAALRVAARG
                     DLRGPVVTVAADLWDRYRSTAWMKAWMDREIAAIALPPPA"
     gene            complement(443..2893)
                     /locus_tag="VF516_02185"
     CDS             complement(443..2893)
                     /locus_tag="VF516_02185"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8106501.1"
                     /translation="MTAGAKLARRYASWIEHRRWAIIACSLAFTAVAAFGASRLRILA
                     DFSYLLPQDVRSVTDLRAIGKRARVLGTAMVAVQSSDPAIRERAAVLLRDKIAGLPLV
                     SSVTFDDHARREYGWQNRWLFADLSDLQRAKAAIEAKIRQAKLDANPLFVGLDDEPAT
                     PRRDAADELRERLRKAEAEVAERGELISSDGKLQMMIVRTAFSSDDVDRDKQLIAGIN
                     QIIDQVRIAVPGADMGVAGDVVVSIAEHDSILNGMLLVTAVTVVLVMAALIWFFRSAL
                     AVGALSWSLAIGTIATFAFTELVIGHLNLATAFLCSIVIGNGINVGILVTARYLEELR
                     AGRDGIEAMAGALANTVAGTLAAALTAAVAYASLMITVFRGFRHFGVIGGAGILLCWC
                     AAYLVLPAALSIARRFGMRPRGEPPIGRWLARMLPQNLAVTAVVTIALIVGAGVITAH
                     YLADDPFESNFRNLRSRGAAITEEQRWMHAIDVAFGQGIDAGFVIAVPHRLDVLPLKA
                     RLRAADADAAPRDKLFARLDSLDDLLPGQQTDKLVVLDQIRELLSSKEIETLSDAERA
                     ELARLRPPDDLHALGDNDVPDALAWPFIEADGSRGKVILAMAGPGYEVWDAHDTVRFA
                     DKVRALHLPDDVHLGGASFVFADVLEAVLTDGPRATIASLLGAILVVLIVVGRNRHAL
                     VTIACGLGGTMLMLGTAALLGLKINFLDFVALPITIGIGIEYAVNIVTRERQEGPGQG
                     RAALATTGGAVVICSYTTIVGYGSLWASPNLGIVSFGKAALLGELTCLGVALFLAPAL
                     LWITAPALARRRSEVPTR"
     gene            3078..4292
                     /locus_tag="VF516_02190"
     CDS             3078..4292
                     /locus_tag="VF516_02190"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8106502.1"
                     /translation="MPTPVPISEGRLRICMMVTYDMASPGGGVKQHALHLAASLRRLG
                     DEVMLVGPSSVPTDDPDVHSFGGIVNIPANGSDNNLGLLVSPRAIARFFREHHFDVIH
                     IHEPLQPALSYWSVWLTRRVPHVATFHAFMEEEGLLLRWARKAWGATMFPWIQRAIAV
                     SEPAARYASAAWNRELSIIPNGVPTDIFTPLKHHDHHHHAHSGRVKLLFVGRIGDVRK
                     GARYLFEAYGKLAASGLPVTLDVVGELGQAEPPPQLPGLTYHGAVAFDRLAALYRNCD
                     IFVAPSTGQESFGIVLLEAMASAKPVVCSDIEGYRQVATTEGSTLVPPSNAGALADAL
                     AQVVRIDPEMRRRHGELNRRHAELFDYDRLAERVRHEYRLAIAACRAGAAPRVEPAAR
                     PIRPVRSVGSED"
BASE COUNT          678 a         1581 c         1534 g          604 t
ORIGIN      
        1 gtcggcaagc ggatcaagga gcgcgatccc gaggccaagg tcgtgctcgc cgatccggtc
       61 ggcagctcgc tcgccgactg ggtcaacacc ggcacgctcg gacccgacgg gccgtacacg
      121 gtcgagggca tcggctcggg gtcggtcccc ggcaacctgc accgcgacgc gctcgactac
      181 gccgagaagg tgccggacgc ggaggcctac gcgatggtcg agcggctggt caaggaggag
      241 gggctgacgg tcggcggctc gaccggcgtc aacgtggtcg cggcgctgcg ggtcgcggcg
      301 cgcggcgacc tgcgcggccc ggtggtcacg gtcgcggccg acctctggga tcgctaccgc
      361 tcgacggcgt ggatgaaggc gtggatggac cgggaaatag ccgccattgc gctgccgccg
      421 ccggcctgac gcacgggggc cgctaccgcg tcgggacctc ggaccggcgg cgcgccagcg
      481 cgggcgccgt gatccacagc agcgccggcg cgaggaacag cgcgacaccc aggcaggtca
      541 gctcgccgag caacgccgcc ttgccgaacg agacgatgcc gaggttcggc gacgcccaca
      601 gcgagccgta gccgacgatc gtggtgtacg agcagatcac gaccgcgccg ccggtggtgg
      661 cgagcgcagc ccggccctgc cccgggccct cctggcgctc gcgggtcacg atgttgaccg
      721 cgtactcgat gccgataccg atcgtgatcg gcagcgcgac gaagtcgagg aagttgatct
      781 tgagcccgag cagcgccgcg gtgcccagca tcagcatcgt gccgccgagc ccgcacgcga
      841 tggtcaccag ggcgtggcgg ttgcggccga ccacgatcag cacgaccagg atcgcgccga
      901 gcagcgacgc gatggtggcg cgcggcccgt cggtcagcac ggcctcgagc acgtcggcga
      961 acacgaacga cgcgccgccg aggtggacat cgtccggcaa atgcagcgcg cggaccttgt
     1021 cggcgaaccg cacggtgtcg tgggcgtccc acacctcgta gcccgggccg gccatcgcga
     1081 ggatcacctt gccgcgcgag ccgtcggcct cgatgaacgg ccaggccagc gcgtcgggca
     1141 cgtcgttgtc gccgagcgcg tggaggtcgt cgggcggccg cagccgggcg agctcggcgc
     1201 gctcggcgtc ggacagggtc tcgatctcct tggatgacag gagctcgcgg atctggtcga
     1261 gcacgaccag cttgtcggtc tgctggcccg gcagcagatc gtcgagcgag tcgagccggg
     1321 cgaacagctt gtcgcgcggc gccgcatcgg cgtcggccgc gcgcagccgg gccttgagcg
     1381 gcaggacgtc gagccggtgc ggcaccgcga tcacgaaccc cgcgtcgatg ccctggccga
     1441 acgcgacgtc gatcgcgtgc atccagcgct gctcctcggt gatcgccgcg ccgcgcgacc
     1501 gcaggttcct gaagttgctc tcgaacggat cgtcggccag gtagtgcgcg gtgatcacgc
     1561 ccgcgccgac gatcagcgcg atcgtcacga ccgcggtgac cgcgaggttc tggggcagca
     1621 tccgcgccag ccagcgcccg atcggcggct cgccgcgcgg ccgcatgccg aaccggcgcg
     1681 cgatcgacag cgccgccggc agcacgagat acgccgcgca ccagcacagc aggatgccgg
     1741 cgccgccgat cacgccgaag tggcggaatc cgcggaacac cgtgatcatc agcgacgcgt
     1801 aggcgaccgc cgcggtcagc gccgcggcca gcgtgccggc gaccgtgttg gccagcgcgc
     1861 cggccatcgc ctcgatgccg tcgcgcccgg cccgtagctc ctcgaggtag cgcgcggtca
     1921 ccaggatgcc cacgttgatc ccgttgccga tcacgatcga gcacaggaac gcggtcgcga
     1981 ggttgaggtg gccgatcacc agctcggtga acgcgaacgt cgcgatcgtg ccgatcgcga
     2041 gcgaccacga cagcgcgccg accgcgagcg cggagcggaa gaaccagatc agcgccgcca
     2101 tgaccaggac caccgtcacc gcggtcacca gcagcatgcc gttgaggatc gagtcgtgct
     2161 cggcgatcga caccacgacg tcgccggcca cccccatgtc ggcgcccggg acggcgatcc
     2221 ggacctggtc gatgatctgg ttgatcccgg cgatcagctg cttgtcgcga tcgacgtcgt
     2281 ccgacgagaa cgcggtgcgg acgatcatca tctgcagctt gccgtcggag ctgatcagct
     2341 cgccgcgctc ggcgacctcg gcctcggcct tgcgcagccg ctcgcgcagc tcgtcggccg
     2401 cgtcgcgccg gggcgtcgcc ggctcgtcgt ccagcccgac gaacagcggg ttggcatcga
     2461 gcttggcctg acggatcttc gcctcgatgg ccgccttggc gcgttgaaga tctgacagat
     2521 cggcaaacag ccaccggttc tgccatccgt actcgcgccg ggcgtggtcg tcgaacgtga
     2581 cgctggacac cagcggcagc ccggcgatct tgtcgcgcaa gaggaccgcg gcccgctccc
     2641 ggatcgccgg atcgctggac tgcacggcga ccatcgcggt gccgagcaca cgcgcacgct
     2701 tgccgatcgc acgcaggtcc gtgacggagc gaacgtcctg cggcaacagg taggagaagt
     2761 ctgcaaggat ccggagccgc gaggcaccga acgcggcgac ggccgtgaac gccagcgagc
     2821 acgcgatgat cgcccagcgg cgatgctcta tccaagaggc ataccgcctc gccagcttcg
     2881 caccggccgt cacgggcgca ccatgcccag ggggaacggc tttgtccagc acgcgagctc
     2941 ccggtcagag tgaacccgca tactggccct gtgcacagtg gggcgcggat acggcctcac
     3001 ggttgcacgt ccgacttgac gcaaccgaag gtttggatac cgtgcaccac cccgctatgg
     3061 ccctccgcaa tgcccccatg ccgacgccgg tcccgatcag tgagggacgg ctgcgcatct
     3121 gcatgatggt gacgtacgac atggcctcgc cgggcggcgg cgtgaagcag catgcgcttc
     3181 acctcgcggc ctcgctgcgc cggctcggcg acgaggtcat gctggtcggc ccgtcgtcgg
     3241 tcccgaccga cgaccccgat gttcattcgt tcggcgggat cgtcaacatc cctgccaacg
     3301 ggtccgacaa caacctcggc ctgctggtgt cgccacgagc gatcgcccgg ttctttcgtg
     3361 agcatcactt cgacgtcatc cacattcacg aaccgctcca gcccgcgctg tcgtactggt
     3421 cggtctggtt gacccgccgg gtgccgcatg tcgccacgtt ccatgccttc atggaggagg
     3481 agggcctgct gctgcgctgg gcgcgcaagg cgtggggtgc gacgatgttt ccctggatcc
     3541 agcgggcgat cgcggtctcc gagcccgcgg cgcggtatgc ctcggcggcc tggaaccgcg
     3601 agctgtcgat catccccaac ggggtcccga ccgacatctt caccccgctc aagcaccacg
     3661 accaccacca ccacgcgcac agcggccgcg tcaagctgct gtttgtcggc cggatcggcg
     3721 acgtccgcaa gggcgcccgc tacctgttcg aggcctatgg caagctggcc gccagcggcc
     3781 tgccggtgac cctcgacgtg gtcggcgagc tcggccaggc cgagcccccg ccccagctgc
     3841 ccgggctgac ctaccatggc gcggtcgcgt tcgaccggct cgctgcgctg taccgcaact
     3901 gcgacatatt tgtcgcacct tcgaccggcc aggagtcgtt cggcatcgtg ctgctcgagg
     3961 cgatggcctc ggccaagccg gtggtgtgca gcgacatcga gggctaccgc caggtcgcca
     4021 ccaccgaggg ctcgaccctg gtcccgccgt cgaacgccgg cgcgctcgcc gacgcgctcg
     4081 cccaggtggt ccgcatcgac ccggagatgc ggcgccggca cggcgagctc aaccgccgcc
     4141 acgccgagct gttcgactac gaccggctcg ccgagcgagt tcgccacgag taccgcctgg
     4201 cgatcgccgc gtgccgcgcc ggcgccgccc cgcgggtcga gcccgccgcc cggccgatcc
     4261 ggcccgtccg ctcggtcggg tcggaggact agctcagtgc aagagcttgg gccagcgcat
     4321 gcgcaggatc ggggcgagat ggttcgaagc cgcgccgacc gccagccccg cgaacatgtc
     4381 gacgacccag tggtggc
//
LOCUS       DASVOR010000105         3118 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_380893,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000105 DASVOR010000000
VERSION     DASVOR010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3118
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380893"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..936)
                     /locus_tag="VF516_02195"
     CDS             complement(<1..936)
                     /locus_tag="VF516_02195"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX8106503.1"
                     /translation="MAPLAAACDLDEVDEVALGRGELRAATEIDGGRRQLRLSFPDQW
                     MSTRHARIKRTTGGFWVADEGSKNGLLLNGERTNGALLVDGDWIEAGHTLLRYRHGAL
                     PPPRHPRASRSSGGVALSTMLPNLEEQSRNLEEIARSTVSVILRGETGTGKEVLARAV
                     HQLSGRPGELIGVNCGALPATLLESELFGYRKGAFSNAVEDRPGLVRAADRGTLFLDE
                     IGDLPPSSQAVLLRVLQEREVTPLGGTRPVKVDLRTVCATHRELEMQAALGEFRADLY
                     ARLSGHTFSIPPLRERREDLGLLTAALLRQIAPEQD"
     gene            1560..2231
                     /locus_tag="VF516_02200"
     CDS             1560..2231
                     /locus_tag="VF516_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106504.1"
                     /translation="MNLEHALDRRRSRIAGTPRNDQETIGLERSPDSMDKAWHRLLRF
                     LANGDTQAAAVYEYARNRLLQFFAARGTMGGEELADATFDRVAAKLSDDVAAQVRCPI
                     GYVLRFAHFIHLEHIKSEIARRHRLAALQRDDADDMENAREEWLRNERLALVERCLDE
                     LAPVERTLLLSYYMCDGRSRIVSRQKMSRELGITPALLRTRVSRLLSALNRRVRALAE
                     ETALA"
     gene            2420..>3118
                     /locus_tag="VF516_02205"
     CDS             2420..>3118
                     /locus_tag="VF516_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEX8106505.1"
                     /translation="MIIEKFSLKVLDAVDRAGRLAVKHGHRHTTEWHLFAAMLQQDDT
                     HLKQLLSSAEVDLDVLSHKIDTRLIGLPRAGASDHTTPVSRTLERVFVNAEEHASADA
                     EKYIRINHLMLGLLDDADLRSMIDEIGGRRRDLIRTLREPRTGSDATPPAAARGNNGT
                     SAAPAASANDAVAAHGATPAPTPAAAPAAIESEALAKYARDLTAAARAGELDPLIGRD
                     AEVQLAIEILSRRRK"
BASE COUNT          539 a         1084 c         1025 g          470 t
ORIGIN      
        1 gtcctgctcg ggcgcgatct ggcgcagcag cgccgccgtg agcaggccga gatcctcgcg
       61 ccgctcgcgc agcggcggga tcgagaaggt atggccggac aggcgggcgt acaggtcggc
      121 gcgaaactca ccgagcgccg cctgcatctc gagttctcgg tgggtggcac agacggtgcg
      181 caggtcgacc ttgaccgggc gggtaccgcc gagcggcgtc acctcgcgct cctgcagcac
      241 ccgcaggagc actgcctgcg aggacggcgg caggtcaccg atctcgtcga gaaacagcgt
      301 tccgcgatcg gcggcgcgca ccagacccgg gcgatcttcg acggcgttgg agaacgcacc
      361 cttgcggtac ccgaacagct cgctctcgag gagcgtggcc ggcagggcgc cgcagttgac
      421 gccgatcagc tcgcctgggc gacccgacag ctggtgcacg gcgcgcgcca gcacctcctt
      481 gcccgtgccg gtctcgcccc gcaagatcac cgacaccgtc gagcgtgcga tctcttcgag
      541 gttgcgcgac tgttcctcca ggttcggcag catcgtcgac agcgccaccc cgccgctgga
      601 cctggaagcg cgcggatgcc tgggcggcgg cagcgcgcca tggcgatagc gcagcagtgt
      661 gtgaccggcc tcgatccagt cgccgtcgac cagcagcgcg ccgttggtgc gttcgccatt
      721 gagcaagagc ccgttcttcg agccctcgtc agccacccag aatccaccgg tggtccgctt
      781 gatgcgggcg tgtcgcgtcg acatccattg atcggggaag ctgagccgca actgccggcg
      841 gcccccgtcg atctccgtcg ccgctctcag ctcgccccgt ccgagggcga cctcgtcgac
      901 ctcgtcgagg tcgcaggcgg cggcgagcgg cgccatcggt gcgtcgcagg agaacacgtg
      961 aaacagccac gcgcgcggcc gattcaccga ctcgcggcgg acacgcgcga tctcggagcg
     1021 cgtccggttc ccgctgttgc tcacgtcgtc atgataagcc ggcgtgcact cacgggcatg
     1081 ctggacgagc tccccatcgc accttatgtg gagattgcgt acatataact acctgtcgca
     1141 gccatgtgac tccacaacgg ggcctggctt cgctgaccag caccaaaggc aagggctcga
     1201 gcgcagcgtc gagccgcttc ggtccggcca gtcccgacga caaatcacga aatctcgcgg
     1261 gtttcggggg ttggccccgg ggcatcctgt cgcgagcgtg cgccgcgaca acccttgcat
     1321 cccgctgtag caggtcgtgg caggcaggtt gtggacaggt tgtggcaggc aggttggggc
     1381 agccatcgcg atccgggagc cgcgccagcg gatacggtct gatcctgcga actctatagc
     1441 gtttccgcaa tacctcgatt gcatgatcac gatccggcca tcgagcactc gacgatcgca
     1501 tctgcagtcg cactccacgc gcgaacgacg gcacccgtct tgctctgcaa ggaagtattg
     1561 tgaatctcga gcacgcactg gatcgcagac gctcgcgtat tgccggaact cctcgcaacg
     1621 accaggaaac gatcggactg gagcgctcgc cggactcgat ggacaaggca tggcatcgcc
     1681 tgttgaggtt cctggccaac ggcgacaccc aagcagccgc cgtttacgag tacgcgcgca
     1741 accgcttgct gcagttcttc gctgcgcggg gcacgatggg aggcgaggag ctcgcggacg
     1801 ctaccttcga ccgtgtcgcc gccaagctgt ccgacgacgt cgccgcgcag gtgcgctgcc
     1861 cgatcggcta cgtgctgcgc ttcgcgcact tcatccatct cgagcacatc aagagcgaga
     1921 tcgcccggcg tcaccgactc gcggcgctgc agcgcgatga tgccgatgac atggagaatg
     1981 cgcgcgagga atggctccgg aacgagcggc tggcgctggt ggagcgctgc ctcgacgagc
     2041 tggcgccggt ggagcggaca ctgttgctct cgtactatat gtgcgacggc aggtcgcgca
     2101 tcgtgtcccg ccagaagatg agccgagagc tcggcatcac gcccgcgctc ctgcgtacgc
     2161 gggtgtcgcg cttgctcagc gcgctcaacc ggcgcgtgcg cgcattggcc gaagagacgg
     2221 cgctggccta gggcgtcgcg atatccgatg cgatcgtggc gatatcactg ctacggatgc
     2281 gccggtagag ccactcgcgt tggacgccag cgatgacgcc cgcagatcga gacgacgcaa
     2341 gatgtccgcc agagagagca gataggaaca ccattcaagc ccgaccggac cggacgggag
     2401 agccgaggag caacgactca tgatcatcga gaaattcagc ttgaaggtgc tcgacgccgt
     2461 cgaccgcgcg ggccgcctcg cggtcaagca cgggcatcgc cacacgacgg agtggcacct
     2521 gttcgcggcg atgctgcagc aagacgatac ccacctcaag cagctgctgt cgtctgccga
     2581 ggtggatctc gacgtcctca gtcacaagat cgacactcgc ctgatcggcc tgccgcgcgc
     2641 cggcgcgagc gatcacacca ccccggtcag ccgcacactg gagcgcgtct tcgtcaacgc
     2701 cgaggagcac gcctcggctg acgccgagaa gtacatccgg atcaaccacc tcatgctcgg
     2761 cctgctcgac gacgccgatc tgcgctccat gatcgacgag atcggcgggc ggcgccgcga
     2821 cctcatccgc accctgcgcg agccgcgcac cggctccgac gccactcctc cggccgcggc
     2881 ccggggcaac aacggcacga gcgctgcacc ggcagcctcg gcgaacgacg cggtcgccgc
     2941 ccacggcgca actccggcac ctacgcccgc tgccgcgccg gcagctatcg agagcgaggc
     3001 gctggccaag tacgcccgcg atctcaccgc cgcggcgcgc gccggtgagc tggatccgct
     3061 gatcggccgc gacgccgagg tccagctggc catcgagatc ctcagccgcc gccgcaag
//
LOCUS       DASVOR010000106         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410258,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000106 DASVOR010000000
VERSION     DASVOR010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410258"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="VF516_02210"
     CDS             <1..627
                     /locus_tag="VF516_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8106506.1"
                     /translation="VADRQVKGFLVEKGTPGYDARRIDGKGSLRAVWQAEIALTDVRV
                     PEENRLPGANSFKDAGRVLAGTRSSVAWAALGHATAAYEIAVGYCMQRRQFGKPLVSF
                     QIVQDRLVTMLAEICSMQLYCLRLGRLAEEGTLTDTIAAIAKMNNTRKARQVISAARD
                     LLGGNGILLDHHVMRHLADIESIHTYEGTETIQTLIVGRDITGVGAFA"
     gene            698..1435
                     /locus_tag="VF516_02215"
     CDS             698..1435
                     /locus_tag="VF516_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8106507.1"
                     /translation="MLLITGLGVSLDVAAPFERELTARSVRTISFDAPGTGRSAAYAW
                     PRRMSGVARTVESMLDVLGYEWVDVLGVSLGGVIAQQLAHQAPDRVRRLVLAATGPGL
                     GGVPGSPRALLPLLTPRRYRRPDYFHRIAGRVYGGDARRDPDTVLRHIVTRIEPPSAW
                     GYLGQLYAITGWTSLRWLRTLRQPTLVLAGDDDPIVPLLNARILAQRIPRARLQIIRG
                     GGHLFLLERPGATADLVAEFLRPGGAA"
     gene            complement(1487..>1832)
                     /locus_tag="VF516_02220"
     CDS             complement(1487..>1832)
                     /locus_tag="VF516_02220"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX8106508.1"
                     /translation="RRAADAQLRIADRITAFAGSMPFVYIHVVVFAGWMLFVESSPWP
                     TLTLIVSLEAIFLSTFVMIGQNRQAAFQQAKADHEFQEEELELKTNTELTREIHRLTS
                     ELHRRLLPKPTV"
BASE COUNT          270 a          670 c          603 g          289 t
ORIGIN      
        1 gtcgcggacc ggcaggtgaa gggattcctg gtcgagaagg ggacacccgg atacgacgca
       61 cggcgcatcg acggcaaggg gtcgctgcgc gcggtgtggc aggccgagat cgccttgacc
      121 gacgtgcgcg tgcccgagga aaaccggctg cccggcgcga actcgttcaa ggacgcgggt
      181 cgggtgctgg ccggcacccg cagctcggtc gcctgggcgg cgctcggcca cgccaccgcg
      241 gcgtacgaga tcgccgtcgg ctactgcatg caacgccggc agttcggcaa gccactggtg
      301 agcttccaga tcgtgcagga tcgactggtc acgatgctcg ccgagatctg ctcgatgcag
      361 ctgtactgcc ttcgcctcgg ccggctggcc gaggagggca cgctgaccga cacgatcgcc
      421 gccatcgcga agatgaacaa cacccgcaag gcgcggcagg tcatctccgc cgcccgcgac
      481 ctgctcggcg gcaacgggat cctgctcgac caccacgtga tgcggcacct ggccgacatc
      541 gagtcgatcc acacctacga gggcacggag accatccaga ccctcatcgt cggccgggac
      601 atcacgggtg tcggcgcctt tgcctgagcc gacagtccgg ttcgtcgacg tcgacggcct
      661 ccggctgcgc acctccgtgc gcggctccgg ccgcccgatg ttgctcatca ccggcctggg
      721 cgtcagcctc gacgttgccg cgcccttcga gcgcgagctc accgcgcgca gcgtgcggac
      781 gatcagcttc gacgcccccg gcaccgggcg atcagccgcc tacgcctggc cgcgacgcat
      841 gtccggcgtg gcccgcaccg tcgagtcgat gctcgacgtc ctcgggtacg agtgggtcga
      901 cgtgctcggc gtctccctcg gcggcgtgat cgcccagcag ctggcccacc aggccccgga
      961 ccgggtgcga cgactcgtgc tcgcggccac cggtcccggc ctcggtggcg taccggggtc
     1021 tccgcgggcg ctgctgcccc tgctcacacc gcggcgctac cgccgaccgg actacttcca
     1081 ccgcattgcc gggcgtgtct acggcggtga cgcgcgccgt gatccggaca ccgtcctgcg
     1141 tcacatcgtc actcggatcg aacccccctc ggcctggggc tatctcggcc agctctacgc
     1201 gatcaccggt tggaccagcc tgcgctggct gcggacgctg cgccagccga ccctcgtgtt
     1261 ggccggtgac gacgacccga tcgtgcctct cctcaacgcc cgcattttgg ctcaacgcat
     1321 cccccgcgcg cgcctgcaga tcatccgcgg cggcggccat ctgttcctcc tggaacgccc
     1381 aggcgcgacg gccgacctgg tcgccgagtt tcttcgccct ggcggggccg catgacctga
     1441 ccgtcgcgga ccgctgtgcc gcggcaaacc actcgggacc tcggcgtcag accgtcggct
     1501 tcggcaggag tcgtcggtga agttcgctgg tcagcctgtg gatctcgcgg gttagttcgg
     1561 tgttggtctt cagctcgagc tcctcctcct ggaattcgtg atccgctttg gcttgctgga
     1621 aggccgcctg ccggttctgg ccgatcatga cgaacgtcga caggaagatc gcctcgagcg
     1681 acacgatcaa cgtcaacgtc ggccacggcg aggactcgac gaacagcatc cagccggcga
     1741 acaccacgac atggatgtac acgaacggca tggagccggc gaacgccgtg atccggtccg
     1801 cgatccgcag ctgcgcgtca gccgcccgcc gc
//
LOCUS       DASVOR010000107         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205635,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000107 DASVOR010000000
VERSION     DASVOR010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205635"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..2544)
                     /locus_tag="VF516_02225"
     CDS             complement(55..2544)
                     /locus_tag="VF516_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX8106509.1"
                     /translation="MRTGAPAGSRGRGRSGGSQPGFRLEAQRGGPPWMAVVKWLAILG
                     VAGAALVAATAAFVFWMYGRDPNLPDYQTLADYHPKQVTVIADGNDRRIGEIYSERRT
                     VVPYDKVPPIVVDAFVAAEDNSFWTHRGVDYWGMFRALLTNLRAGHTRQGASTITQQV
                     VKTFLLTPERTFRRKLQEIILARRLESSLTKQEILTLYMNQIYFGHGRYGVEEAARFY
                     FGKHVGELNIGEAAMLAGLPQSPENISPRKNPKRAKERQTYVLNQLAAMQKITVAEAQ
                     KWIDAPIRVVDRPFPELGSAPEWVEIAKRELLKRYSGNAEESALDRAGTTVRTTLDPS
                     LQAVAQKALQTGLRALDKRHGVGRPVRSVKPDKIEAEIAKLAKRLPRGGPVAKEVYEA
                     VVTHVSDEDRELVVDLGDFPASILLGGEDDARYSPPDADGKTKGPSERFKPGDVVSVM
                     LAAAPAAKPAGKAADEDDEDTRPAKAAAPKHAKHRAVFAPGPEGAVVIIDLKSRKVRA
                     LVGGYASKVAGFDRATMAHRQPGSSFKPYLYATAIDSGRYTPASVLNDAPESFDAFPK
                     WRPKNFETGHYEGPVRLRYALSRSINTVSLELAYNLKPEAVVAFAHKMGVASELTPEL
                     SIALGSNEVTPLEHTNAVATLATGGIAAPPQFIDAIGGKRVAPPAGEQVVRPEVAYVV
                     TDMMRSVVTEGTAASAAALKIPIAGKTGTSNDARDTWFIGLTPDYAIGVWIGHDDNRS
                     LGRETGGSAAVPVYIDIMKQLSPPAKAFARPPHVVEATIDKKTGLLAPDGAPKDATLT
                     EVFVEGTQPTEVAPMPGDVTEGNKTKGEYED"
     gene            complement(2733..>3425)
                     /locus_tag="VF516_02230"
     CDS             complement(2733..>3425)
                     /locus_tag="VF516_02230"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX8106510.1"
                     /translation="HFGAKTIQPASTAWIVKFRAREDEPGSAALEHAYFGMAKAAGVD
                     VPRTRLLGKTKRSPGYFAIERFDRKAATRIHAHTLGGLLQLPHGYPALDYGDLLKVTR
                     RLTRNEAAVAEMFRRACFNVFAHNRDDHCRNFSFLMDEQGRWAPSPAYDLTFAPGPGG
                     EHTMLIAGEARAPGAAHLAALAIGAELKHGARIIDEVRAATSRFQRFAEQAGVPVRLA
                     KRVADALARVPR"
BASE COUNT          429 a         1269 c         1165 g          562 t
ORIGIN      
        1 ccgccgcgag cccgagcgcg accgccagtg cgacggctgg cacgccgcgc cccatcagtc
       61 ttcgtactcg cccttggtct tgttaccctc ggtgacgtcg ccgggcatcg gcgcgacctc
      121 ggtcggctgc gtgccctcga cgaacacctc ggtcagcgtg gcgtccttcg gggcgccgtc
      181 gggcgcgagc agcccggtct tcttgtcgat cgtggcctcg accacgtggg gcggccgcgc
      241 gaacgccttg gccggcgggc tcagctgctt catgatgtcg atgtagaccg gcaccgccgc
      301 gctgccgccg gtctcgcggc ccagcgagcg gttgtcgtcg tggccgatcc acacgccgat
      361 cgcgtagtcc ggggtcagcc cgatgaacca cgtatcgcgg gcgtcgttcg acgtgccggt
      421 cttgccggcg atcgggatct tgagcgccgc cgcgctggcc gcggtgccct cggtgaccac
      481 cgagcgcatc atgtcggtca ccacgtaggc gacctcgggc cgcaccacct gctcgcccgc
      541 cggcggcgcc acccgcttgc cgccgatcgc gtcgatgaac tgcggcggcg ctgcgatccc
      601 gccggtcgcc agcgtcgcga ccgcgttggt gtgctcgagc ggcgtgacct cgttcgagcc
      661 cagcgcgatc gacagctccg gggtcagctc gctcgcgacc cccatcttgt gggcgaacgc
      721 gaccacggcc tcgggcttga ggttgtacgc gagctcgagc gagaccgtgt tgatcgaccg
      781 cgacagcgcg tagcgcagcc ggaccgggcc ctcgtagtgg ccggtctcga agttcttggg
      841 ccgccacttg gggaacgcgt cgaacgactc cggcgcgtcg ttgagcacgc tcgccggcgt
      901 gtaccggccg ctgtcgatcg ccgtggcgta caggtacggc ttgaacgagc tgccgggctg
      961 gcggtgcgcc atcgtcgcgc ggtcgaaccc ggcgaccttc gacgcatagc cgccgaccag
     1021 cgcgcggacc ttgcgcgact tgaggtcgat gatcaccacc gcgccctcgg ggcccggcgc
     1081 gaacaccgcg cggtgcttgg cgtgcttggg cgcggccgcc ttggccggcc gggtgtcttc
     1141 gtcatcctcg tccgctgcct tgccggccgg ctttgccgcg ggcgccgccg ccagcatcac
     1201 cgacaccacg tcgcccggct tgaaccgctc gctcggcccc ttggtcttgc cgtcggcgtc
     1261 cggcgggcta taccgcgcgt cgtcctcgcc gccgagcagg atcgacgccg ggaaatcgcc
     1321 gaggtcgacc accagctcgc ggtcctcgtc ggacacgtgg gtcaccacgg cctcgtagac
     1381 ctccttggcc accggcccgc cgcgcggcag ccgcttggcc agcttcgcga tctcggcctc
     1441 gatcttgtcc ggcttgacgc tgcgcaccgg ccggccgacc ccgtggcgct tgtcgagcgc
     1501 gcgcagcccg gtctgcagcg ccttctgcgc caccgcctgg agcgacggat ccagcgtggt
     1561 gcgcaccgtc gtcccggccc ggtccagcgc cgactcttcg gcgttgccgc tataccgctt
     1621 gagcagctcg cgcttggcga tctcgaccca ctccggcgcg ctgccgagct cggggaacgg
     1681 ccggtcgacc acccggatcg gcgcgtcgat ccacttctgg gcctcggcga ccgtgatctt
     1741 ctgcatcgcc gcgagctggt tcagcacgta ggtctgccgc tccttggccc gcttggggtt
     1801 cttgcgcggc gagatgttct ccggcgactg cggcagcccg gcgagcatcg ccgcctcgcc
     1861 gatgttgagc tcgccgacgt gcttgccgaa gtagaaccgc gccgcctcct cgaccccgta
     1921 gcggccgtgg ccgaagtaga tctggttcat gtacagcgtc aggatctcct gcttggtcag
     1981 cgaggactcg agccgccgcg ccaggatgat ctcctggagc ttgcgccgga acgtgcgctc
     2041 gggcgtgagc aggaaggtct tgaccacctg ctgggtgatc gtcgaggcgc cctgccgcgt
     2101 gtgaccggcg cgcaggttgg tgagcagcgc ccggaacatc ccccagtagt cgacgccgcg
     2161 gtgggtccag aagctgttgt cctcggccgc gacgaacgcg tcgaccacga tcggcggcac
     2221 cttgtcgtac ggcaccacgg tgcgccgctc gctgtagatc tcgccgatcc ggcggtcgtt
     2281 gccgtcggcg atcaccgtga cctgcttggg gtggtagtcg gcgagcgtct ggtagtccgg
     2341 caggttcgga tcccgcccgt acatccagaa cacgaacgcc gcggtcgccg cgaccagcgc
     2401 cgcgcccgcg accccgagga tcgcgagcca cttgaccacc gccatccagg gcgggccgcc
     2461 gcgctgcgcc tccagccgga accccggctg ggatcccccg gatcggccgc gtccgcgcga
     2521 ccccgccggg gcgcctgtcc tcatcgtcga gcccatgggt gatacgcctg caagtgtgat
     2581 accgcctgta accgaggctg ccccggcatt gtccctcggt tgcgtcttcg atgccacatc
     2641 ccggcgtgtc tgcaacgaca cgcccgataa gtccacgccg ccttcgggtt acccggctcc
     2701 aagcgcggct cgggatcgcc atggcagcgc ggtcagcgcg gcacgcgcgc gagcgcgtcg
     2761 gccactcgct tcgcgagccg gaccggcacc ccggcctgct ccgcgaaccg ctggaaccgg
     2821 ctggtcgccg cccggacctc gtcgatgatc ctcgcgccat gcttgagctc ggccccgatc
     2881 gcgagcgctg cgaggtgcgc ggcgcccggc gcgcgggcct cgccggcgat cagcatcgtg
     2941 tgctcgccgc cgggaccggg cgcgaaggtc agatcgtacg ccgggctcgg cgcccaccga
     3001 ccctgctcgt ccatcaggaa cgagaagtta cggcaatggt cgtcgcggtt gtgcgcgaac
     3061 acgttgaagc acgcccggcg aaacatctcg gccaccgccg cctcgttgcg ggtcagccgg
     3121 cgggtcacct tgagcagatc gccgtagtcg agcgccggat agccgtgggg cagctgcagc
     3181 agcccgccca gcgtgtgcgc gtggatccgc gtggccgctt tgcgatcgaa gcgctcgatc
     3241 gcgaagtagc ccgggcttcg cttggtcttg ccgagcaatc gcgtccgcgg aacgtctacc
     3301 ccggctgcct tggccatccc gaagtacgca tgctccagcg cggcgctgcc gggttcgtcc
     3361 tcgcgcgccc ggaacttcac gatccacgca gtggacgccg gctggatggt cttcgcgccg
     3421 aaatg
//
LOCUS       DASVOR010000108        14772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_468694,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000108 DASVOR010000000
VERSION     DASVOR010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14772
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468694"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..912
                     /locus_tag="VF516_02235"
     CDS             <1..912
                     /locus_tag="VF516_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106511.1"
                     /translation="RELEPEATWRVRGHELLDGAAPDLISRATEAAARALGWQDAGST
                     QAFLDALAAAPSRIIGVPLPAPPPYHRAGMTLSVEIDRGDVWRDAAPRWHDAPRRSAL
                     ILYALDGEQRIPLVRWPTTIGGWQWQKVDGDVEERWKESPVGPRVWRDLFVGPRWLPP
                     DSTPDRELVRGSDGHAVLAREQLGPSYRAAFGLVAFVHLVSEQEHGQVVLEDQGIRTH
                     GTGNLTSLANGVSHGCHRLLGRDVVRLADFVLAHHDHVRHGEAPTYYRRVVRYGASFP
                     IAIDSLGYRIELVPPIPVDVLPGRIHR"
     gene            1114..1710
                     /locus_tag="VF516_02240"
     CDS             1114..1710
                     /locus_tag="VF516_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106512.1"
                     /translation="MNSRREPTAVRPPGVIDMASVPFECQLFATLPRDSTSELLPWAQ
                     SLSMAACRQSITLAPVSDPEQFRPMIARLEQAMAPSIAMYRDALARGPMQIKILAAYG
                     LGMVNVNIIVWARSAVHIPGGDPSFGGASYGGTSYVARSQRLHQLLEALLVKERDAAL
                     SAFHDVARLAHEDPRAAQANQVMAFVVMNAQMQAALLR"
     gene            1788..2237
                     /locus_tag="VF516_02245"
     CDS             1788..2237
                     /locus_tag="VF516_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8106513.1"
                     /translation="MSHPAVTCHVNDDLTIAAKLMWDHDCGVVLVVRDDGKLAGVITD
                     RDICMAAYTQGRSLDQILVNSVMAKHVVLAHPDQKLGEVEHMMAEHQVRRIPVVDDDN
                     RPVGVLSLNDLALESVEPDTRMKDGVSKVAHTLAAVCRPRVEKQVAA"
     gene            2639..3865
                     /locus_tag="VF516_02250"
     CDS             2639..3865
                     /locus_tag="VF516_02250"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106514.1"
                     /translation="MGCVFLADQPALRRSVAIKVLHPELAGIPAHARRFQDEAVIACH
                     VRSPHCIEVFDSGVLLDGTPYIVMEHIPGRPLGRIIADEPIPLSRAIDLFDQVLRALA
                     ATHRSGILHGDVKSDNFLVEIGDGVDHVTLIDFGLGQAIAAPARVDRDHGEILISGTP
                     EYLAPERIDGDPASPASDLYGAGVILYELLTGTTPFGGGTAMEIMNRQVRDPVIPPSQ
                     RRPDRDIPPVIDRIVLRALDKRPGARFADAEAFARALQAAAPRHTLRQPVAFVGPIQR
                     VPGDDTIARRSRRRLARGSDCGGRGGDQARTIRQEIGGALRRGDVAAIADGYLRLGNA
                     LVGEGQFWCAIHELQEGIDVLTAGGDPRASDAPHGVDRLAVALAALYETAGDRQLARH
                     AATSTDHSPTWTLAIG"
     gene            4002..4889
                     /locus_tag="VF516_02255"
     CDS             4002..4889
                     /locus_tag="VF516_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8106515.1"
                     /translation="MTFRKLLCPVDFSPGSQHALQVAARMAREADAELVLVHAWYVPT
                     TAFGGDYPIPVDLAQEVVDGAQRDLAAARTDATRLGAPRVSSRVLRGVPWDQIVQAAR
                     EDPAIDLIVMGTHGRSGLARVLLGSVAEQVIRHAPCSVLAARAPGGVQPFRKVLCPID
                     FSEDSRHALDRAAELAARTGAGITLLHVIEPAATFSNLPMADNNLQAIDRRATHELAR
                     WASELENKVSIPVNTEIRIGRPGVQTLAVLDADPAFDLVVMGSHGRTGIRRVLLGSVA
                     EKVLRHAACPVLIVRSRGV"
     gene            complement(4864..5445)
                     /gene="rrtA"
                     /locus_tag="VF516_02260"
     CDS             complement(4864..5445)
                     /gene="rrtA"
                     /locus_tag="VF516_02260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HEX8106516.1"
                     /translation="MAIAATASAFASAGIADLLVADPRIAHGQLWRALSGPLVHATWG
                     HLVRDLALVAIAGAAYEAPLRALRTPLFVGGLVLPGLAVLAAGDAQWYCGLSGLSHAL
                     LAAALAYELVRRRGTARAIVLALCAVSVVKPIYELVTGTPAFAMSLGDDVVQVPLAHV
                     AGVLAGIACGLLAGLASDRTCAAGPATRLANGR"
     gene            complement(5499..7706)
                     /locus_tag="VF516_02265"
     CDS             complement(5499..7706)
                     /locus_tag="VF516_02265"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX8106517.1"
                     /translation="MSRVRLGLALLSLVAGCGPLATGSPGSSSVGTYTSPTVHSSSGV
                     EATGNDNFAAMDEVPVGGGELRLVEPRRTQDDSGAIVTAIMFPLRHTDVQTKVSGMSA
                     MYTVTQTFENPYDQPIDAVYVFPLGDEAAITSYAIVIGERTIAGEIKTKEEARATYEE
                     ARAQGHTAALLEQEKRNIFRQRIANIAPRETIKVRMQYIELLGYQDGQYEITFPLVVG
                     PRYLPAGAVGSTPVGAHHAGRQGRADVTSIPYADARVAGSTVSFTADVDAGVPVLSVS
                     SPSHQLVVDDVATTRRKIALASAGELPNRDLIVRYRTASEQTMVGLLAHRSDAKGYFT
                     LVVQPKASYRTGDITPREVMIVIDTSGSMDGPPLAQARALASALIDALRPGDTFNAMA
                     FSGSTNAMAPSAIAGDGIGKQTGKGFVASLTSGGGTEMGPAMARALAAAPGNDRIRLV
                     FFLTDGFVGNDDMIVSAARGNLGANRIFSVGIGSAPNRSLLNQIATVGRGFASYLNLT
                     DPAADLAEDLVRRTGFPYLTDIQIDWNGLAVGAVTPRAIPDVYAGMPLVVSGVYTRPG
                     RARITVSATTAGRRVQIPVEVTLPDRVDAEPVAALWARKRVDELLSVAGDHVTAETVS
                     QITELGLGFHMVTDYTSFVAVDRTRVVAQNGASQIIEQPALAPAGVNLETAIGPSAQT
                     ASYASSGSPSGGSESSGGGGWGGHGGGGDVDLLTLVLAVCLIPLAWTLRRIRA"
     gene            7807..8025
                     /locus_tag="VF516_02270"
     CDS             7807..8025
                     /locus_tag="VF516_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106518.1"
                     /translation="MAARFASNVIGDGRSAGPSGEPLVLEARGDVLGIHTAMDEFVEE
                     ATVFGLSEAEVDGERCLDVFDVVPEQPA"
     gene            complement(8240..8395)
                     /locus_tag="VF516_02275"
     CDS             complement(8240..8395)
                     /locus_tag="VF516_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106519.1"
                     /translation="MQKKKPIKQLKPLRLRKEALKVLTNDQQLRVHAGSDPWGDQQTA
                     YPICQNA"
     gene            complement(8713..9705)
                     /locus_tag="VF516_02280"
     CDS             complement(8713..9705)
                     /locus_tag="VF516_02280"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8106520.1"
                     /translation="MPRVIQRRYGNALIVDNADYRVMVSSEAVRKRYPSEADKLRVAL
                     VDLTGNKLCRPGYAGWGSTWPMPGGSTAKIAIVYAAHQLLFDLAEMARAGGISTAAAL
                     KSAAASAWSGLSCKPDVDWLVTIDASTTPVGVAASANLTRHLDEMVDASFSDVSTSRA
                     SELILRIGFEYIASLMWQSGLRHPTRAGLWLGNLFMDVSFTAKTSPTCHSGTNPVSWK
                     QNPLGATGITLTGLSAATYFTLLAQGRLVNPTTSTRIEALLRKGCRFISIPGATVRAT
                     KCGLTSSVRHDAALIENGSRRYVMVVLTKNASWSLNVRDRFLNDLDRLIKDNNP"
     gene            complement(9743..12145)
                     /locus_tag="VF516_02285"
     CDS             complement(9743..12145)
                     /locus_tag="VF516_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106521.1"
                     /translation="MKPLSPSSLSAPCDPATLPFVSTVELEARDEIVGQAQALRALDL
                     GLTVRADGYNLFVAGAPQTGKRSILRRLVRARAAAEPTSPDLCYVHNFDDPDRPLVLE
                     LPAGRSRPIRLELEHLVSDYARRLPLAFDGEAFAQRAAAITERTARERDRLLAEIEAR
                     LGSSGFKLETTGGALDVAVAAPDGSVMSEEAFAALPAAKRAEIDERGRALRSELEPFA
                     ARLRELERGAELEREQLATETASTIARAVTDEARARIGDAGARLASHLDAIQHDLVSE
                     AASFAAVEPVGDGDHEDDESPAVRHARRVAERAALLARYVLHVIVDRTGETGAPVIEE
                     ANPTYANLFGRIERKATLGVLETSVAGLKAGALQRARGGYLLLDAAPLVDDAETWAAL
                     KRALHSGEVRIEEQGSEHLVVGSLQPEPSPLDTKLLLIGVPELYLALFEADEEFVDLF
                     KVKVELDDTLERSPAHELALARFIAAAAHQLGVPGLQAGAVAALIDHASREAGHQRRL
                     SARLGRLRDLVVEAGHYTRVAGRTEVVADDVARAVRERLDRSGLLERTLGRLVDDGTL
                     VARSEGTAVGAANALSVISDGERAFGQVQRVTARARAGRPGIVDIERQIGLAGAHHAK
                     GVLMLSGFLAGRFVLDRTLALQASIAAEQTHGVIEGDSASLAELCALLSAIGGVPLRQ
                     DLAVTGAVSQLGEVLAVGEVTLKVEGFFDLCKRRGLTGSQGAIIPRANVQHLMVRAEV
                     VDAARGGQFAIYAVDDVDQALALLGGRDAGARGADGRFPAGSINAAIEDVLDRWAPAA
                     KP"
     gene            complement(12254..12742)
                     /locus_tag="VF516_02290"
     CDS             complement(12254..12742)
                     /locus_tag="VF516_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106522.1"
                     /translation="MSACALAQLFGAGFVAARQLGAAAIVDPRPHAVGRIAELYRRYS
                     HVGAALPAVGYSPEEIRDLEATIRATECDSVVIATPADLGHVLRLDRPATRVRYELAD
                     HGTPTLADEIRRFLADRPSRCRRSPPPRRAGWRLPESSRPAAWAPRSTPRSTFSPPGS
                     AC"
     gene            complement(12809..13225)
                     /locus_tag="VF516_02295"
     CDS             complement(12809..13225)
                     /locus_tag="VF516_02295"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8106523.1"
                     /translation="MSTHSVPSVSHVMTAQPWTIERDATLRDAHALMQAHQIRHLPVL
                     EGGRLVGLVTDRDLRLLEATTPVDAATTPVDRAMTDRPFIVTSDTALDEVVSIMSDHK
                     YGSAIVVGRDGVEGIFTSVDACRVLAELLQREQIEM"
     gene            13622..13816
                     /locus_tag="VF516_02300"
     CDS             13622..13816
                     /locus_tag="VF516_02300"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HEX8106524.1"
                     /translation="MAARRMLFGRFDVCIENHRLVATAWGEPVDISRHQPVVEPECAT
                     MTALRVAREATWLAQTVIDV"
     gene            14347..>14772
                     /locus_tag="VF516_02305"
     CDS             14347..>14772
                     /locus_tag="VF516_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106525.1"
                     /translation="MHRMPISRRWSGAISAYRDALQSGGHLGPRLARTRAMTVDLQDI
                     SIPIGDIELEGTLAIPTAVTGLVMFAHGSGSSRHSPRNRRVASLLHERHLATLLFDLL
                     TVDEERIDAVDATLRFDVEMLAERLVAATDSITAAGHSRG"
BASE COUNT         2245 a         5092 c         5183 g         2252 t
ORIGIN      
        1 cgcgagctcg agcccgaggc gacctggcgg gtgcgcggcc acgagctgct cgacggcgca
       61 gctcccgacc tgatctcgag ggcgaccgag gccgccgccc gcgcgctggg ctggcaggac
      121 gccggcagca cccaggcatt cctcgatgcg ctcgcggccg ccccgtcgcg gatcatcggc
      181 gtgccgctgc ccgcgccgcc gccctaccac cgcgcgggga tgaccctgtc ggtcgagatc
      241 gatcgcggcg acgtctggcg cgacgccgcc ccgcgctggc acgacgcgcc ccggcgatcg
      301 gcgctgatcc tctatgcgct cgacggcgag cagcggatcc cgctggtgcg ctggccgacc
      361 acgatcggcg gctggcagtg gcagaaggtc gacggcgacg tcgaggagcg ctggaaggag
      421 tcgccggtcg gcccgcgggt gtggcgcgac ctgttcgtcg gcccgcgctg gctcccgccg
      481 gattccaccc cggaccgcga gctggtccgc ggctcggacg gccatgccgt gctcgcccgc
      541 gagcagctcg gcccgagcta ccgcgctgcg ttcggcctgg tcgcattcgt ccacctcgtg
      601 tcggagcagg agcatgggca ggtggtcctg gaggaccagg ggatccgcac gcacggcacc
      661 ggcaacctga cctcgctcgc gaacggcgtc agtcacggct gccaccgcct gctcggccgc
      721 gacgtcgtcc ggctggcgga cttcgtgctc gcccatcacg accatgtccg gcacggagaa
      781 gcgccgacct actatcgccg cgtcgtccgc tacggcgcca gcttcccgat cgcgatcgac
      841 tcgctcggat atcgcatcga gctcgtcccg ccgatcccgg tcgacgtgct cccggggcgg
      901 atccatcgct gacgcagcac gcaggtacgg ccgcgagggc atgcgccccg atcacgcagg
      961 atttgcagct tcatggacag ccctgcgcga gtccgctggt cctcgaaatg caggtcgagg
     1021 atcgcaagca accggagaat gaccatgaaa cgcgcacatt cgtcctggat tgtcgtcctg
     1081 ctcgcaatca ttgccggtac gtcggcggcc gatgtgaaca gcaggcgcga gccgactgcg
     1141 gtccgtcccc cgggcgtgat cgacatggcc agcgtgccgt tcgaatgcca gctgttcgcc
     1201 acgctcccgc gggactcgac cagcgagctt ctgccgtggg cgcagtcgct ctcgatggcg
     1261 gcgtgccggc agtcgatcac gcttgccccg gtcagcgatc cggagcagtt ccggcccatg
     1321 atcgcgaggc tcgagcaggc gatggcgccg tcgatcgcga tgtaccgcga cgctctggcg
     1381 cgcggcccga tgcagatcaa gatcctcgcc gcctacggcc ttggtatggt gaacgtgaac
     1441 atcatcgtct gggcgcgcag cgcggtccat atccccggag gcgacccgag cttcggcggc
     1501 gcgagctacg gcggcacgag ctacgtcgcc cgctctcagc ggctccatca gctcctcgag
     1561 gccctgctcg tgaaggagcg cgatgccgcg ctgtctgcgt tccacgacgt cgctcgcctg
     1621 gcccacgagg atccgcgcgc ggcccaggcc aaccaggtca tggccttcgt ggtcatgaat
     1681 gcgcagatgc aggctgcgct gcttcgttga tcgccacctg gtccgatctc tgcacagatg
     1741 tctgcactac ttcgaaggag agaagacaca tgcgagttca ggatctcatg agccatccag
     1801 cagtcacctg ccacgtcaac gacgatctca ccatagcggc caagctcatg tgggatcacg
     1861 actgcggcgt ggtcctggtc gtgcgcgacg acggcaagct ggcgggagtc atcaccgacc
     1921 gtgacatctg catggcggcg tacacccagg ggcgttcgct cgaccagatc ctggtcaact
     1981 cggtcatggc gaagcacgtc gtcctggcac atccggatca gaagctcggc gaggtcgagc
     2041 acatgatggc cgagcaccag gtgcggcgga tcccggtcgt cgacgacgat aaccgccccg
     2101 tcggcgtact gtcgctgaac gatctcgcgc tggagagcgt ggagccggac acccggatga
     2161 aggacggcgt gtccaaggtc gcccacacgc tggctgccgt gtgccggccg cgtgtcgaga
     2221 agcaggtcgc ggcatagcct gtatcggggc gcacccggga acgggctctg cacgtctacc
     2281 cgctcgcgat gatgccgcgc tggctccagg ccgtcgagca cgacgcggga tccggccacg
     2341 atgcgggcgc cgatctcgtc ctcttcgccg aagcggcctc gcacgaggca tgccacagcc
     2401 ggtaacagag acaccgaggg gaaacgcgcg agttctgcga atcgacgcgc tcgctccggt
     2461 tggccttgcg cgaagcgagc gtttgtcatc cagctggaac attcagcgcc gcgggcgccg
     2521 ggcgacgttc gccgcaggta cgccgtttgc tttgcaatgc gtcatgcagt cggggttgaa
     2581 gccaggcgat gtggtcgatg gcagatacgt gcttcgcgag cgcatcggtc aggggggcat
     2641 gggatgtgta ttcctggcgg accagccggc gctccggcgc agcgtcgcga tcaaggtgct
     2701 tcatcccgag ctcgccggca ttcccgccca tgctcgcagg ttccaggacg aggccgtgat
     2761 cgcgtgccat gtccggagtc cacactgtat cgaggtgttc gactccggtg tcttgctcga
     2821 tggcacgccg tacatcgtga tggagcacat ccccggcaga ccgctcggcc ggatcatcgc
     2881 ggacgaaccg atcccgctgt cccgggccat cgacctgttc gatcaggtcc tccgcgcgct
     2941 cgccgccacc catcgcagcg gcatcctcca tggcgacgtg aagagcgaca atttcttggt
     3001 cgagatcggc gacggcgtcg accacgtcac cctgatcgac ttcggtctcg ggcaggccat
     3061 cgcggcgccc gctcgcgtcg atcgggacca cggcgagatc ctgatctcgg gtacgcccga
     3121 gtacctggca cccgagcgga tcgacggcga cccggcgagc cctgcgtcgg acctgtacgg
     3181 ggcaggtgtc atcctctacg agctgttgac cggaacgacg ccgttcgggg gtggcacggc
     3241 catggagatc atgaaccggc aggtccgcga cccggtgatc ccgccgtctc agcgccggcc
     3301 cgatcgcgac atccccccgg tgatcgaccg cattgtcctg cgcgcgctcg acaagcgtcc
     3361 cggagcgcgg ttcgcggatg ccgaagcctt tgcgcgcgcg ctgcaggcgg ctgcgccgcg
     3421 ccacacgttg cgccagccgg ttgccttcgt cggtccgata cagcgggtgc ctggcgacga
     3481 cacgattgcg cggcgatcgc gccgccgcct cgcgcgcgga tccgattgcg ggggacgggg
     3541 cggcgaccag gcgaggacga tacgccagga gatcggcggc gcgctgcggc gcggtgatgt
     3601 cgccgcgatc gccgatggct atctccggct cgggaatgcg ctggtcggag agggccagtt
     3661 ctggtgcgcg atccacgagc tccaggaagg catcgacgtc ctcacggcag gcggcgaccc
     3721 gcgcgcaagc gatgcaccgc atggtgtcga ccggctcgcc gttgcgctgg cggcgctgta
     3781 cgagacggca ggcgatcggc aactcgcccg gcacgcggcc acgagcaccg atcacagccc
     3841 gacctggacg ctcgcgatcg gttgaatccg ccggcatcgc gtcgctcatg cagcgcggcc
     3901 tcggagcgcc cgcagcgctt gcgccggtgc gcaggccggt gcaggaagtt gcgcgaccgg
     3961 tcggccgctg cgatggccgc gcggttgcat caccagctac catgacgttc aggaagctgc
     4021 tgtgccccgt cgacttctct ccagggtcgc aacatgcgct gcaggttgcg gcgcgcatgg
     4081 cgcgcgaggc cgatgccgag ctggtgctcg tccacgcctg gtacgtgccg acgacggcgt
     4141 tcggcggcga ctacccgatc cccgtcgatc tcgctcagga ggtcgtggac ggcgcgcagc
     4201 gcgacctcgc cgctgcgcga accgacgcga ccaggctcgg tgctccacgc gtctcgtccc
     4261 gcgtactacg cggcgtgccc tgggaccaga tcgtccaggc cgcgcgcgaa gatcctgcga
     4321 tcgacctgat cgtcatggga acacatggcc gcagcgggct cgcccgggtg ctgctgggct
     4381 cggtcgccga gcaggtgatc cgccacgcgc catgctcggt gctcgccgca cgcgcgccgg
     4441 gcggtgtgca accgttccgc aaggtcctgt gcccgatcga cttctccgag gattcccgtc
     4501 atgcgctgga ccgcgccgcc gagctcgctg cgcgcactgg agccggtatc accctgctgc
     4561 acgtgatcga gcctgcggcc acgttcagca acttgccgat ggcggacaac aacctccagg
     4621 cgatcgatag acgcgcgacc catgagctcg cgcgctgggc atcggagctg gagaacaagg
     4681 tctccatccc ggtgaacacc gagatccgga tcggtcgccc cggtgtccag acgctcgccg
     4741 tgctcgatgc agatccggcc ttcgatctcg tcgtcatggg cagtcatggc aggacgggaa
     4801 tccggcgggt tctgctcggc tcggtggccg agaaggtgct gcgccacgcg gcctgcccgg
     4861 tgctcatcgt ccgttcgcga ggcgtgtagc ggggcctgca gcgcacgtcc gatcggaggc
     4921 cagccccgcg agcaacccgc acgcgatgcc agcgagcacg ccggcgacgt gcgcgagcgg
     4981 gacctgcacg acgtcatcgc cgagcgacat cgcgaacgcg ggcgtgccgg tcaccagctc
     5041 gtagatcggc ttgaccacgc tcaccgcgca cagcgcgagc acgatcgcgc gggcagtgcc
     5101 gcggcgccgg accagctcgt acgccagcgc cgccgcgagc agcgcgtgtg acagccccga
     5161 tagaccgcag taccactgcg catcaccggc ggcgagcacg gcgagcccgg gcagcacgag
     5221 cccgcccacg aacagcggtg tgcgcagcgc gcgcagcggc gcctcgtagg cggcccccgc
     5281 gatcgcgacc agcgcgaggt cgcgcaccag gtggccccag gtcgcgtgga ccagcggtcc
     5341 ggacagcgcg cgccacagct ggccgtgcgc gatccgcgga tcggcgacca ggaggtccgc
     5401 gatgcccgcc gacgcgaacg cgctcgccgt ggcagcgatc gccatccccg agactgcgag
     5461 cgtccacgga aagtcgcagc accggcgagc gcgtgcggtc acgcgcggat ccggcgcagc
     5521 gtccatgcca gcgggatcag gcagacggcg aggaccagcg tcaagaggtc gacgtcgccg
     5581 ccgccgccgt gaccgcccca gccaccgccc ccgctcgact cgctcccgcc cgacggcgac
     5641 cccgagcttg cgtacgaggc ggtctgcgcc gacgggccga tcgcggtctc gaggttgacg
     5701 ccggcaggtg ccagcgcggg ctgctcgatg atctggctcg cgccgttctg cgcgacgacg
     5761 cgcgtgcgat cgacggcgac gaacgaggtg tagtcggtga ccatgtggaa cccgagcccc
     5821 agctcggtga tctgcgagac cgtctcggcc gtgacatggt cgccggcgac ggacagcagc
     5881 tcgtcgacgc gcttgcgcgc ccacagcgcg gcgaccggct cggcgtcgac ccggtctggc
     5941 agggtgacct cgacggggat ctgcacgcgg cggccggcgg tggtcgcgct caccgtgatc
     6001 ctggcgcggc cgggccgggt atagaccccg gacaccacga gcggcatacc ggcgtagacg
     6061 tcggggatcg cgcgcggcgt gaccgcgccg accgcgaggc cattccagtc gatctggatg
     6121 tcggtgaggt acggaaatcc ggtgcgtcgc accaggtcct cggcgagatc tgccgccggg
     6181 tcggtgagat tcaggtagct cgcaaagccg cggccgaccg tggcgatctg gttcagcagc
     6241 gagcggttgg gcgccgagcc gatgccgacg ctgaagatcc ggttggcgcc gaggttgccc
     6301 cgcgccgcgc tgacgatcat gtcgtcgttg ccgacgaagc catcggtcag gaagaacacc
     6361 aggcggatgc ggtcgttgcc gggcgcagcc gccagggccc tggccatcgc ggggcccatc
     6421 tcggtgccgc cgcccgacgt cagcgacgcg acgaagccct tgccggtctg cttgccgatg
     6481 ccgtcgcccg cgatcgccga cggtgccatc gcgttggtgc tgccggagaa cgccatcgcg
     6541 ttgaacgtgt cgccgggccg gagcgcatcg atcagcgcgc tggccagcgc gcgggcctgc
     6601 gcgagcggcg ggccgtccat cgagcccgag gtgtcgatca cgatcatcac ctcgcgcggg
     6661 gtgatgtcgc cggtcctgta gctcgccttg ggctggacga cgagcgtgaa gtagcccttc
     6721 gcgtcgctgc ggtgcgcgag caggcccacc atggtctgct ccgacgccgt gcggtagcga
     6781 acgatgaggt cgcggttggg gagctcgccc gcgctcgcca gcgcgatctt gcgccgggtg
     6841 gtcgcgacgt cgtcgaccac gagctggtgc gaggggctcg acaccgacag caccggcacg
     6901 ccggcgtcga cgtcggcggt gaagctgacg gtcgaacccg cgaccctggc gtcggcgtac
     6961 gggatcgagg tcacgtccgc ccggccctgg cgcccggcgt gatgcgcgcc gaccggtgtg
     7021 ctgccgaccg cgccggccgg caggtagcgc ggaccgacga cgagcgggaa cgtgatctcg
     7081 tactgcccgt cctgatagcc gagcagctcg atgtactgca tgcgcacctt gatcgtctcg
     7141 cgcggtgcga tgttcgcgat ccgctgccgg aagatgttgc gcttctcctg ctcgagcagc
     7201 gcggcggtgt ggccctgcgc cctggcctcc tcgtaggtcg cgcgcgcctc ttccttggtc
     7261 ttgatctcgc cggcgatcgt gcgctcaccg atcacgatcg cgtagctggt gatggccgcc
     7321 tcgtcgccga gcgggaacac gtagaccgca tcgatcggct ggtcgtacgg gttctcgaac
     7381 gtctgcgtga cggtgtacat cgccgacatc cccgacacct tcgtctgcac gtcggtgtgg
     7441 cgcagcggga acatgatcgc ggtgacgatc gcgcccgaat cgtcctgggt ccggcgcggc
     7501 tcgacgagcc ggagctcgcc acctccgacc gggacctcgt ccatggcggc gaagttgtcg
     7561 ttgccggtgg cctcgacacc gctgctggag tggaccgtcg ggctggtgta cgtgccgacc
     7621 gacgagctgc cgggcgagcc cgtcgccagc ggcccacagc cggcgaccag cgagagcaag
     7681 gcgagaccga gacggacgcg agacatggtg cctcctggat ctggacccca caaagctgcg
     7741 tgtggtccga cctctgcgcc cgttcgatca cgcgttgttc acgaagcacc accggattcc
     7801 cgcgcgatgg ccgctcgctt cgcgtcaaac gtgatcggcg atggccgctc agccgggccc
     7861 agcggcgaac cgctcgtcct ggaagcacgc ggcgacgttc tggggataca tactgcgatg
     7921 gatgaattcg tcgaagaagc gaccgtgttt ggtctgtccg aagcggaagt ggacggagag
     7981 cgatgtctcg acgtattcga cgtagtgcca gaacagccag catgaagatg ggcagccacg
     8041 gatcccctcg agatgccccg gccatggtgc ggctctatct cgagatgaga cggccaaccc
     8101 tgcagcgcat gctgggtctg gtgcaggcac cggggcggca ggtacaagca gtgccgcaaa
     8161 gggtgagtcc gccgacggag agtggcagcg aacccgatga agagctatcg cctgacccgc
     8221 atcatcgggt cagtcgaatt catgcgttct ggcagatagg gtaggccgtc tgctggtcac
     8281 cccaggggtc actgccggcg tgtacgcgga gctgctggtc attcgtgagg accttgaggg
     8341 cttccttgcg aagacggagg ggcttcagct gcttgattgg cttcttcttt tgcatggcga
     8401 gacggtagtg gtttctcgta ttgaagtaaa ggcgtcctca tgaaggttca tcgcctacaa
     8461 cctgccggtc ggtaacccat gctggctatc gtgcgcatcc ggtcggaaac atggcgggag
     8521 tcggtcaact gccccgcggt agtcgacgtg acgaagcggg cattctcgag gacccgctcc
     8581 cgccggggcc agaacgcgcg tggccgcgct cttcacctct ggagccgaac gacagcaccg
     8641 gcttccggca aaagtggttg caaggcttga accgtcgcca ccttgcagcg acggcgccga
     8701 accgtctcgt cgctatggat tgttgtcttt gatgagtcgg tcgaggtcgt tgaggaagcg
     8761 atcgcgcacg ttgagcgacc agctcgcgtt cttggtcagg acaaccatca cgtagcggcg
     8821 ggagccgttt tcgatcagcg cggcgtcgtg ccgcacggag ctggtgaggc cgcacttcgt
     8881 tgcgcgcacg gtcgcgccgg ggatcgagat gaacctgcat cccttgcgca gcagcgcctc
     8941 gatccgcgtc gaggtcgtgg ggttgaccag gcgcccctgc gcgagcagcg tgaagtaggt
     9001 cgccgccgac aggccggtga gcgtgatgcc ggtggcgccc agcgggttct gcttccagga
     9061 caccgggttc gtgccgctat ggcaggtggg gctggtcttg gccgtgaacg acacgtccat
     9121 gaacaagttc ccgagccaga ggccggcgcg cgtgggatgg cggaggcccg attgccacat
     9181 cagcgaggcg atgtactcga agccgatgcg caggatgagc tcgctggccc gcgaggtgct
     9241 gacgtccgag aacgaggcgt cgaccatctc gtcgaggtga cgggtgaggt tggcgctggc
     9301 cgccaccccg accggcgtcg tgctggcatc gatcgtcacg agccagtcga catccggctt
     9361 gcacgagagt ccggaccagg cgctcgcggc agcgctcttg agggcagcgg cggtgctgat
     9421 gccacccgca cgggccatct ctgcgaggtc gaacaggagc tggtgcgcgg cgtacacgat
     9481 ggcgatcttc gccgtgctgc cgccgggcat gggccaggtc gatccccagc ccgcgtaccc
     9541 gggcctgcac agcttgttgc cggtgaggtc gaccagcgcc acgcggagct tgtcagcctc
     9601 gctggggtag cgcttgcgga cggcttcgct gctcaccatg acccggtagt cggcgttgtc
     9661 gacgatgagg gcgttgccgt atcgccgctg gatgaccctg ggcaagcacg tgtagcttga
     9721 cgcccacgcc ggcgggcttc cgctatggct tggcggcggg cgcccaccga tcgagcacgt
     9781 cctcgatcgc ggcgttgatg ctgccggccg ggaatcgccc gtcggccccg cgcgcgcccg
     9841 cgtcgcggcc gccgagcagg gcgagcgcct ggtccacgtc gtcgaccgcg tagatcgcga
     9901 actggccgcc gcgcgccgcg tcgacgacct cggcgcgcac catcaggtgc tggacgttcg
     9961 cgcgcggaat gatcgctccc tgggacccgg tcagaccgcg tcgcttgcac aggtcgaaga
    10021 agccctcgac cttgagcgtg acctcgccga ccgcaagcac ctcgccgagc tggctgacgg
    10081 cgccggtcac ggcgaggtcc tggcggagcg gtacgccgcc gatcgcggag agcagcgcgc
    10141 acagctcggc gagcgacgcg gagtcgccct cgatcacgcc gtgggtctgc tcggccgcga
    10201 tcgaggcctg cagcgcgagg gtgcgatcga gcacgaaccg cccggcgagg aaccccgaga
    10261 gcatcagcac acccttggca tggtgcgcgc cggcgagccc gatctggcgc tcgatgtcga
    10321 cgatcccggg acggccggcg cgcgcacgcg cggtcacgcg ctggacctgg ccgaacgcgc
    10381 gctcgccatc ggagatcacc gatagcgcat tggccgcgcc gaccgcggtg ccctcgctgc
    10441 gtgcgaccag ggtgccgtcg tcgaccagcc gcccgagcgt gcgctcgagc aagccgctgc
    10501 gatcgagccg ctcgcggacc gcgcgagcga cgtcgtcggc gactacctcc gtgcgaccgg
    10561 cgacccgcgt gtagtggccg gcctcgacca cgagatcgcg cagccggccg aggcgcgccg
    10621 acagccggcg ctggtggccg gcctcgcgcg acgcgtgatc gatgagcgcg gcgaccgcgc
    10681 cggcttgcaa cccgggcacg ccgagctggt gcgcggccgc cgcgatgaac cgggcgagcg
    10741 ccagctcgtg cgccggcgag cgctcgagcg tatcgtcgag ctcgaccttg accttgaaga
    10801 gatcgacgaa ctcctcgtcg gcttcgaaca gcgcgaggta aagctcgggc acgccgatca
    10861 ggagcagctt ggtgtcgagg ggtgacggct ccggctggag gctgccgacc accaggtgct
    10921 cgctgccctg ctcttcgatc cggacctcgc cgctgtgcag cgcgcgcttg agcgccgccc
    10981 aggtctcggc atcgtcgacc agcggcgcgg catcgagcag caggtagccg ccccgcgcac
    11041 gttgcagcgc gccggccttg agcccggcga cgctggtctc gagcacgccg agggtggcct
    11101 tgcgctcgat ccgtccgaac aggttcgcgt acgtcgggtt cgcctcctcg atcacgggcg
    11161 cacccgtctc gccggtgcga tcgacgatca cgtgcagcac gtagcgagca agtagcgccg
    11221 cacgttcggc gacgcggcgc gcgtgccgga cggcgggaga ctcgtcgtct tcgtgatcgc
    11281 cgtcgccgac cggctcgacg gcggcgaagc tcgccgcctc gctgaccaga tcatgctgga
    11341 tcgcgtcgag gtggctggcg agccgcgcgc ctgcatcgcc gatccgcgcg cgggcctcgt
    11401 ccgtgaccgc gcgcgcgatc gtgctcgcgg tctcggtggc cagctgctcg cgctcgagct
    11461 cggcgccgcg ctcgagctcg cgcagccgcg ctgcgaacgg ctcgagctcg gatcgtagcg
    11521 ctcggccacg ctcgtcgatc tcggcgcgct tcgcggccgg caacgccgcg aaggcctcct
    11581 cgctcatcac gcttccatcg ggtgcggcca cggcgacgtc gagtgcgccg ccggtggtct
    11641 cgagcttgaa cccggaggaa ccgagccgcg cctcgatctc cgcgagcaag cgatcgcgct
    11701 cgcgcgccgt ccgctccgtg atcgccgcgg cgcgctgtgc gaacgcctcg ccgtcgaatg
    11761 cgagcggtag ccggcgcgcg tagtcgctca ccaggtgctc gagctcgagc cggatcggtc
    11821 ggctgcggcc ggcaggcagc tcgagtacga gcgggcgatc ggggtcgtcg aagttgtgga
    11881 cgtagcagag atccggcgag gtcggctcgg ccgccgcgcg cgcgcggacc aaccggcgca
    11941 agatcgagcg cttgccggtc tggggggcgc cagcgacgaa caggttgtag ccgtccgcgc
    12001 ggacggtcaa cccgaggtcg agcgcgcgca gcgcctgagc ctggccgacg atctcgtcgc
    12061 gcgcttcgag ctcgaccgtg gacacgaacg gcagcgtcgc ggggtcgcac ggcgcggaca
    12121 gcgaggacgg ggacagcggc ttcatgcgca gctgccagtt accacgaccg ctctccggcc
    12181 gcgcacggtc agggcgtgat ccaggtgccg caacgcccgg cgagcgcgtc ggccaggcgc
    12241 ggcagcgcgg tgatcagcac gcgctccccg gtggcgagaa agtcgagcgc ggcgtcgatc
    12301 ttggggccca tgctgccggc cgggaactct ccggcagccg ccagccggcg cgcctcggcg
    12361 gcggtgagcg ccggcagcgc gacggccggt ccgcgaggaa gcgacggatc tcgtcggcca
    12421 gcgtgggtgt cccgtgatcg gcgagctcgt agcggacgcg agtcgccggg cggtcgagcc
    12481 gcagcacgtg gccgagatcc gcgggcgtcg cgatcacgac actgtcacat tcggtcgctc
    12541 ggatcgtggc ctccagatcg cggatctcct cgggcgagta gccgaccgcg ggcagcgccg
    12601 cgccgacgtg tgagtagcgt cgatagagct cggcgatccg accgaccgcg tgcggccgcg
    12661 gatcgacgat cgcggctgcg ccgagctggc gcgcggcgac gaaccccgcg ccgaacagct
    12721 gggcgagtgc acacgccgac aaacgagccg cccgcccgag ctggccaatg atcgtgcaga
    12781 ctatctctcc tgctgtgacg gcgcccagtc acatctcgat ctgctcgcgc tgcagcagct
    12841 cggcgagcac gcggcaggca tcgaccgacg tgaagatgcc ctcgacgccg tcgcggccga
    12901 ccacgatcgc cgagccgtac ttgtgatcgc tcatgatcga gacgacctca tcgagcgcgg
    12961 tgtcgctggt aacgatgaac ggtcgatcgg tcatcgcccg atcgaccggc gtggtcgcgg
    13021 catcgaccgg cgtcgtcgcc tccagcagcc ggaggtcacg gtcggtgacc agcccgacca
    13081 gacggccgcc ctccagcacc ggcaggtggc ggatctggtg ggcctgcatc agggcgtggg
    13141 cgtcgcgcag cgtggcgtcg cgttcgatcg tccagggctg cgcggtcatg acgtgcgaga
    13201 cggagggcac ggagtgcgtc gacatgcggg ttcaagcatg catcacgcgt gccgatgcgc
    13261 tgctcgggat cgaatggaag tgcgtcaccg gatcctcgat ccaggcgtct cagtcacgtt
    13321 ggcagagagg gcaatcacca tgcctctgat cgctgatcag gccagtgcgc gctgccagcc
    13381 caatgccgag cggctcgcgt ggtcaacctc gcgaatgcgt ctccccgatc gcggtcatga
    13441 catctcgtac agctacagca aacctcgtgc ctacctgttg gctcccacct ccggcaccac
    13501 ggattcgttc tcgtcagcgg tgaccacatc gcggacgcat agctcgccgg ctcgcatgcc
    13561 cctggacaat gcatagtccg ttccacgccg atgcacggga cgacgaacta tcgcggcggc
    13621 gatggcggcg cgccgcatgc tgttcggacg cttcgatgtg tgcatcgaaa accatcggct
    13681 tgtcgccacc gcgtggggcg aaccagtcga catctcccgc caccagccgg tcgtcgagcc
    13741 cgagtgcgcg acgatgaccg cattgcgagt cgcacgcgag gccacgtggc tcgcgcagac
    13801 cgtgatcgac gtgtgaggct gccatgaatc tcactgacct cgttcagcgc gtgcgggttc
    13861 cagaaattct gacctgatcg attccggaaa ttctggccac cccgcgaagc gctggcgcgg
    13921 tcgcgagctc atcgatgcgc tcggccaacg cgggatcctg atccgcagtc cgagcctccg
    13981 cggcgtcgca gaggaggcgc ccgaggcata caaggacgtc ggggccgtgg tcgacgctgc
    14041 gcaggctgcc gggctcgctc gcaaggtcgt tcgcctcgag ccgatgatcg tggtgaaggg
    14101 ctgaaggacg gctcgccgca agtaccgtcg gctcaccgtc caccgatcag cgccggagtc
    14161 tcgcgcgcaa cgtgcgcaag gatggcagcg catcggccgt cgctgcaccg gctcgcgcgg
    14221 tcgaaggtcc tcgacaggag cgggctcgca ggatgggggg ccgcggacac tcgatccacg
    14281 cggtcagcgc acccggcagc aggacaccgc taccatcgcg caggcattgc gcgcgctcct
    14341 cgatcggtgc atcgcatgcc gatatcacga agatggtcag gagccatctc ggcataccgg
    14401 gatgcgctgc aaagtggcgg ccatttgggt ccccggcttg cacggaccag ggccatgact
    14461 gtcgatctgc aagacatctc gattccgatt ggcgacatcg agctcgaggg aacgctcgca
    14521 atccccacgg cggtgaccgg cctggtgatg ttcgcacacg gcagcggcag cagtcggcac
    14581 agcccgcgca accggcgcgt cgcgtcgttg ctccacgagc gccacctggc gacgctgctg
    14641 ttcgatctgc tcaccgtcga cgaggagcgg atcgacgcgg tcgacgccac gctgcggttc
    14701 gatgtcgaga tgctcgccga gcgcctggtg gcggcgaccg atagcatcac cgccgcggga
    14761 cactctcgcg gc
//
LOCUS       DASVOR010000109         6010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410278,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000109 DASVOR010000000
VERSION     DASVOR010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410278"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1188
                     /locus_tag="VF516_02310"
     CDS             157..1188
                     /locus_tag="VF516_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106526.1"
                     /translation="MRQWISRATRLAHATATLAFALGMLAPIAAHGQASPQAEALFRD
                     GKQLMKEGKLAEACAAFEGSERAEHSIATVLSLADCREKNRQYASAWALFLQADSQTR
                     SDPTKAALNTTAKRRAHALEARLSYLTINVPDESRIPDLLVTRDGAPVDPAEWNRSIP
                     ADGGSHVIAGKAPGHEPWSTTVTLQPENDKQAVEVPLFKALPEIVNRPPRDEAAVQVR
                     RAPAPPEPNPLTRMRKIGIGVAAGGAVVGGVAIGLGVSASSLHNDAVASCPPAACSVK
                     NAQDATATNDRARSRARYANIGYGVAGAALVTGGVLWLVGKPESPRGQVVVAPQLGDA
                     SGIAVVGRF"
     gene            1190..3415
                     /locus_tag="VF516_02315"
     CDS             1190..3415
                     /locus_tag="VF516_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106527.1"
                     /translation="MRRTARPGRCTAPALALALGLTACSIPDTAFHATPDAGSGPGSM
                     SDVLAIVVSSPAIELDEGATTDFTVQLNHAPAAPVEIDLSTASQKIGLSIQKLFFDAT
                     NFAQAQTITVTGIRDDDTTTEIASIKLSGAGLADIMVGATVHDPDKVTVVTNVADNTK
                     VTEKTSLDIPVHLSHRPPGDVVVTATLSSGPMTVSPASRVFHMDDSYSTDVVFTVTAP
                     ADANASNEMQTITFGIAGGDQKILQLIDVDVDKLAIVPSPTSIAHLLEGDSVTLNLTL
                     SLPPSTDTTVMVATTSGKAQISATSVTFLAGGTDYKTNHPITITAPQDNNTTPETDTI
                     VLSVPNPPDGKAVDSINIPVQIDDDDVQAIQTTVVNSLTVSENATKDFGVTLKFAPDP
                     SNPVTVNLGTLDAGVATAATPAGANFLTFTATDYNDPTKHPVRVQGTHDNNLVTNSTT
                     VTLTSAAPQMETDVLVDVLDIDQQAFVLTPGTPLSIPEGTSATFTLALAFVPTTTVTA
                     QIASDSAALVIPASSSSFDFTPTSWNKPVTVTVSAPVDKNNVSESGTISIASSPTSPT
                     VPTATVAAKVMDSTTVISYGWPAPPAFTETAGLRASAVIAYKIHIDATNLDTYGIDVP
                     VAAGAYRMALYNDNGNKPDTLVGTVSSQFPATNLVNGVNIIDVPDVQLATGDYWLALR
                     VSATTNVAATTQTTGRRCNADFDIVGLANNWPATFGVSDPTTGCMQASLLNIWITTYH
                     Q"
     gene            3412..5994
                     /locus_tag="VF516_02320"
     CDS             3412..5994
                     /locus_tag="VF516_02320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106528.1"
                     /translation="MKYLLSLSFLLIGTVATAAPQNNVCVPRASGVPTREGPPKWLNW
                     TGSGTIPADTALDDPRWLGATGDTFALGSAKAPLQSRMLFSVDASGQEYLYLSFIVDL
                     DGFDTSAAHPSVTTPRDLFIGFHRPTVPAGATQHGYMFQFHLTGGGSGVVAPQYCGSY
                     ATCDESSPTPHDFWRLFADLGHDGSSVCTGNGGRLYEPLAGATPTTPPVTWVTTAAGD
                     QDAVRFWKVSSSDPTPLLQNRWAVQVRIPVIAPVAGTGSPIVTGVERGSTFFYEATAK
                     LAGPGNGIYAKVGWFPLSITTSVCPDNNTDTILHEELGDPSAGCSTCDPTKFSKLSDL
                     TGAAPADCDAGLDIDADHIGTLFNTPVPADPEVIGPMLGRQFGARDTTTPNHVIALPF
                     NTKPVPGGGGTANDITAPIQARFRLAGWGSAPWSDPTDLGRWKDIRGAASGICGTAPA
                     AGSTDLCGASTIPAQKHGIIKFDWTIGGDTGPGGIGSSEYCEFGLTPPAGSGETCATG
                     CSCADPTDCAAGTGVHASKAGAPGTFWPCVPSIYQFDQCMLVELNAPNGGAHFVHQSI
                     WSNMSFGEMSTLAHEALIDARHLPVAPGQQYQDIYFIAMPRNMPPSVPATSTSVQLAQ
                     NAAINVALRIAQPYLDDLNRTPPLVVERIARSLPRAAAGAASEGDERIRQILRARQIM
                     SVADARRVDGLLQIALRQGGQDNKPSASLVHASVAAVGSSTTAEIVPTLEIYPYYQKL
                     GKGRAYEPMTSFSVFLSHENTLAGIRYQIDGADQVGQNIYHLRIPVGNARKIQIRAQA
                     LTGNEAPLPPGNPVWPCAGGCAACGGVNRSCGLVAAIGSGAPGLLACVWVIGRRRKQP
                     KRRG"
BASE COUNT          992 a         2257 c         1987 g          774 t
ORIGIN      
        1 cgacgcgccg ggcaagcggc cgcaccgccc gcatccgccc gccgtcccgc acggcggctc
       61 cggcagcagc acgactccac ccggttgcga tcgctcgatc gacaccgact gcgatgggat
      121 tccagacgtc cgttaacgtt cgctgagggg taaaagatga ggcagtggat ctcgcgggcc
      181 acgcggctcg cgcacgctac agctacgctc gcgttcgccc tggggatgct cgcgccgatc
      241 gcggcgcacg ggcaggccag cccgcaggcc gaggcgctgt tccgcgacgg caagcagctg
      301 atgaaggagg gcaagctcgc cgaggcgtgc gccgcgttcg agggcagcga gcgcgccgag
      361 cacagcatcg ccaccgtgct cagcctcgcg gactgccgcg agaagaaccg ccagtacgcg
      421 tcggcgtggg cgctgttcct ccaggccgac agccagaccc gctccgaccc gaccaaggcg
      481 gcgctcaaca ccacggccaa gcgccgggcc cacgcgctgg aggccaggct gtcgtacctg
      541 acgatcaacg tgcccgacga gagccggatc cccgacctcc tcgtcacccg cgacggtgcg
      601 ccggtcgatc cggccgagtg gaaccgctcg atccccgccg acggcggcag ccacgtgatc
      661 gccggcaagg cgcccggcca cgagccgtgg tcgaccacgg tgacgctcca gcccgagaac
      721 gacaagcagg ccgtcgaggt cccgctgttc aaggcgctcc ccgagatcgt caacaggccg
      781 ccgcgcgacg aggcggcggt ccaggtccgg cgggcgcccg cgccgcccga gccgaacccg
      841 ctgacgcgca tgcgcaagat cgggatcggg gtcgccgccg gcggcgcggt ggtcggcggc
      901 gtcgcgatcg gcctgggggt cagcgcgagc agcctgcaca acgacgcggt cgcgagctgt
      961 ccgccggcgg cgtgctcggt gaagaacgcg caggacgcca ccgcgaccaa cgatcgcgcc
     1021 cgcagccgcg cgcgctacgc caacatcggc tacggcgtcg ccggcgccgc cctggtcacc
     1081 ggcggcgtgc tgtggctggt cggcaagccg gagtcgccgc gcggccaggt cgtggtcgcg
     1141 ccgcagctcg gcgatgccag cgggatcgcc gtcgtgggga ggttctgaga tgcgccgcac
     1201 cgctcgtccc ggtcgatgta ccgctccggc cctcgcgctg gccctcggcc tcaccgcgtg
     1261 cagcatcccc gacaccgcgt tccacgccac gcccgacgcc ggatccggcc ccggcagcat
     1321 gtccgacgtg ctggcgatcg tcgtgtccag ccccgcgatc gagctcgacg agggcgcgac
     1381 caccgacttc accgtgcagc tgaaccacgc gccggccgcg ccggtcgaga tcgacctgtc
     1441 gaccgcgtcg cagaagatcg gcctgtcgat ccagaagctg ttcttcgacg cgaccaactt
     1501 cgcgcaggcg cagaccatca cggtcaccgg gatccgcgac gacgacacca cgaccgagat
     1561 cgccagcatc aagctgagcg gtgccggcct cgcggacatc atggtcggcg cgacggtcca
     1621 cgatccggac aaggtgacgg tcgtcaccaa cgtcgccgac aacaccaagg tcaccgagaa
     1681 gacgtcgctg gacatcccgg tccacctctc gcaccggccg ccgggggacg tcgtggtcac
     1741 ggcgacgctg tcgagcggcc cgatgacggt ctcgccggcc agccgcgtgt tccacatgga
     1801 tgacagctac agcaccgacg tcgtgttcac ggtcaccgcg ccggccgacg cgaacgcctc
     1861 gaacgagatg cagacgatca cgttcggcat cgcgggcggc gaccagaaga tcctccagct
     1921 gatcgacgtc gacgtggaca agctcgcgat cgtgccgtcg ccgacctcga tcgcgcacct
     1981 gctggagggc gacagcgtca cgctcaacct cacgctgagc ctgccgccgt ccaccgacac
     2041 gacggtcatg gtcgccacca cctcgggcaa ggcgcagatc agcgcgacct cggtcacgtt
     2101 cctcgccggg ggcaccgact acaagaccaa ccacccgatc acgatcacgg cgccgcagga
     2161 caacaacacg accccggaga ccgacacgat cgtgctgtcg gtgcccaatc ccccggacgg
     2221 caaggcggtg gactcgatca acatcccggt gcagatcgac gacgacgacg tccaggcgat
     2281 ccagaccacg gtggtcaact cgctgaccgt gtccgagaac gcgaccaagg acttcggcgt
     2341 gacgctcaag ttcgcgccgg acccgagcaa cccggtcacg gtcaacctgg gcacgctgga
     2401 cgcgggggtc gcgaccgcgg cgacgccggc aggcgccaac ttcctgacct tcacggcgac
     2461 cgactacaac gaccccacga agcacccggt ccgcgtgcag ggcacccacg acaacaacct
     2521 ggtcaccaac tcgaccacgg tgacgctgac gtcggcggcg ccgcagatgg agaccgacgt
     2581 cctggtcgac gtgctcgaca tcgaccagca ggcgttcgtg ctcacgcccg gcacgccgct
     2641 gtcgatcccc gagggcacaa gcgccacgtt caccctcgcc ctggcgttcg tgccgacgac
     2701 cacggtcacc gcgcagatcg ccagcgacag cgcggcgctg gtgatcccgg cctcgtcgtc
     2761 gtcgttcgac ttcacgccga ccagctggaa caagccggtg acggtgacgg tcagcgcgcc
     2821 ggtcgacaag aacaacgtct ccgagagcgg gacgatctcg atcgcatcgt cgccgacctc
     2881 gcccacggtc ccgaccgcca ccgtggcggc caaggtgatg gacagcacga cggtgatcag
     2941 ctatggctgg ccggcgccgc cggcgttcac cgagaccgcc gggctgcggg ccagcgcggt
     3001 gatcgcctac aagatccaca tcgacgccac caacctcgac acctacggga tcgacgtgcc
     3061 ggtcgccgcc ggcgcctacc ggatggcgct gtacaacgac aacggcaaca agcccgacac
     3121 cctggtcggc accgtgtcgt cgcaattccc ggccaccaac ctggtcaacg gcgtgaacat
     3181 catcgatgtt ccggatgtgc agcttgccac gggggactac tggctcgcgc tgcgggtcag
     3241 cgcgacgacc aacgtcgcgg cgaccaccca gaccaccggc cggcgctgca acgcggactt
     3301 cgacatcgtc ggtcttgcca acaactggcc ggcgacgttc ggcgtctcgg atccgaccac
     3361 agggtgcatg caggccagcc tgctcaacat ctggatcacc acgtaccacc aatgaaatac
     3421 ctcctcagct tgtccttcct cctgatcgga accgtggcga ccgcggcccc gcagaacaac
     3481 gtctgcgtgc cgcgcgcgtc gggcgtgccg acccgcgagg gccctccgaa gtggctcaac
     3541 tggaccgggt cgggaacgat cccggccgac accgcgctcg acgatccgcg ctggctcggc
     3601 gcgaccggcg acacgttcgc gctcggcagc gcgaaggcgc cgctccagtc gcggatgctg
     3661 ttcagcgtcg acgccagcgg ccaggagtac ctctacctgt cgttcatcgt cgacctcgac
     3721 ggcttcgaca ccagcgcggc gcacccctcg gtcacgaccc cgcgcgatct gttcatcggc
     3781 tttcaccggc cgacggtgcc ggccggcgcg acccagcatg gctacatgtt ccagttccac
     3841 ctgaccggcg gaggcagcgg ggtggtggcg ccccagtact gtggctcgta cgcgacctgt
     3901 gacgagagct cgccgacgcc gcacgacttc tggcggctgt tcgccgacct cggccacgac
     3961 ggcagctcgg tgtgcaccgg caacggcggc cggctctacg agccgctcgc gggcgcgacc
     4021 ccgacgaccc cgccggtcac ctgggtgacc acggcggccg gcgatcagga cgccgtccgg
     4081 ttctggaagg tctcgtcgtc ggatccgacg ccgctgctgc agaaccgctg ggcggtccag
     4141 gtccggatcc cggtgatcgc gccggtcgcc ggcaccggca gcccgatcgt caccggcgtc
     4201 gagcgcggct cgacgttctt ctacgaggcg accgccaagc tcgccggacc gggcaacggc
     4261 atctacgcca aggtcgggtg gttcccgctg tcgatcacga cctcggtgtg ccccgacaac
     4321 aacaccgaca cgatcctcca cgaggagctc ggcgatccga gcgccggctg ctcgacctgc
     4381 gatccgacca agttctccaa gctgtccgat ctgaccggcg cggcgccggc cgattgcgac
     4441 gcgggcctcg acatcgacgc cgaccacatc ggcacgctgt tcaacacccc ggtcccggcc
     4501 gaccccgagg tgatcgggcc gatgctcggc aggcagttcg gcgcgcgcga caccaccacg
     4561 ccgaaccacg tgatcgcgct gccgttcaac accaagccgg tgccgggcgg cggcggcacc
     4621 gccaacgaca tcaccgcgcc gatccaggcc cggttccggc tcgcgggctg gggctcggcg
     4681 ccgtggtcgg atccgaccga cctcgggcgc tggaaggaca tccgcggcgc ggccagcggg
     4741 atctgcggca cggcgccggc ggccggctcg accgacctgt gcggcgcctc gacgatcccg
     4801 gcccagaagc acggcatcat caagttcgac tggacgatcg gcggcgacac cggcccgggc
     4861 ggcatcggct cgagcgagta ctgcgagttc ggcctgacgc cgccggcggg atccggcgag
     4921 acctgcgcga ccggctgcag ctgcgccgat cccaccgact gcgccgccgg gaccggcgtg
     4981 catgccagca aggcgggcgc tcccggtacc ttctggccgt gcgtgccgtc gatctaccag
     5041 ttcgatcagt gcatgctggt cgagctcaac gcgcccaacg gcggcgcgca cttcgtccac
     5101 cagagcatct ggagcaacat gtcgttcggc gagatgtcga ccttggccca cgaggccctg
     5161 atcgacgccc gccacctgcc ggtcgcgccc ggccagcagt accaggacat ctacttcatc
     5221 gcgatgccgc gcaacatgcc gccgagcgtg ccggcgacca gcaccagcgt gcagctggcg
     5281 cagaacgccg cgatcaacgt ggcgctgcgg atcgcgcagc cctacctcga cgatctgaac
     5341 cgcacgccgc ccctcgtggt cgagcggatc gccaggagcc tcccgcgcgc ggccgccggc
     5401 gcggcctccg agggcgacga gcgcatccgc cagatcctgc gcgcccgcca gatcatgagc
     5461 gtggccgacg cccgccgggt cgacgggctg ctccagatcg cgctgcgcca gggcggccag
     5521 gataacaagc cgagcgcgtc gctggtccac gcctcggtcg ccgcggtcgg ctcgtccacc
     5581 accgccgaga tcgtgccgac gctggagatc tatccgtact accagaagct cggcaagggc
     5641 cgcgcctacg agccgatgac gtcgttctcg gtgttcctca gccacgagaa cacgctggcc
     5701 ggcatccggt accagatcga cggcgccgac caggtcggcc agaacatcta ccacctgcgg
     5761 atcccggtcg ggaacgcccg caagatccag atccgcgccc aggcgctgac cggcaacgag
     5821 gccccgctgc cgccgggcaa tccggtgtgg ccgtgcgcag gcggctgcgc ggcgtgcggc
     5881 ggcgtgaacc ggagctgcgg cctggtcgcg gcgatcggca gcggcgcacc cggcttgctc
     5941 gcctgcgtgt gggtgatcgg gcgccggcgc aagcagccga agcggcgcgg ataggtgtct
     6001 gcagggcggg
//
LOCUS       DASVOR010000110         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410286,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000110 DASVOR010000000
VERSION     DASVOR010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410286"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VF516_02325"
     CDS             <1..319
                     /locus_tag="VF516_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106529.1"
                     /translation="LPTAIGAAAGTLLAGGSLWSVVAALVAAPLAALHPSLTTGMVVG
                     RVEAAWRRPTLTDRERLGDDLQSLAGFRRNPVTRILLVAIASSLGTALGFWFAVGWLV
                     RLL"
     gene            406..1797
                     /locus_tag="VF516_02330"
     CDS             406..1797
                     /locus_tag="VF516_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8106530.1"
                     /translation="MMKRRDALKTLGGLGAAAGMARFLPACGSDGPRGITTYVYLMME
                     NRSYDHLLGARAMAPENKGGDGLPTPTAFSNPDLNGMAVTSFTPDKDSLCALDPPHGW
                     DAARAQWNHGANDGFLRVHQMAHNNDPTARQPMQYLVRSDVPVSWALADAYATSDRWF
                     CAVMGPTFPNRFYWHAASSEGLMSNTLPPGGLMGPSIYHRLDAKGIPWKYYYGNIPLV
                     AAYQGLDVTRLQSFEQFLADARAGTLPPVVYIDPAFDANDDHPPVHPINGQELIASVY
                     NALATSPQWKNCLLLITYDENGGFYDHVSPPTTVDERAAQGFNQLGFRVPAIVAGPYV
                     KQGYLSSLQYEHCSALRELEVAFGLEPINQRMMAARDLGDFIDSERLARGDWAKPITL
                     PTVDPTDMAQWPMGASCKPGADPFPTTRHPAIEWADAHPAVLAGLDLRPQVAAYRRAI
                     RQSLAQPPGALVR"
     gene            complement(1806..2663)
                     /locus_tag="VF516_02335"
     CDS             complement(1806..2663)
                     /locus_tag="VF516_02335"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX8106531.1"
                     /translation="MGPSGSDLRTIPLFRGFSDEALAELGELFTRLIVDESVPLFDIG
                     EPATAMYLLTQGEVVLEQPNEVFRLSPPAIIGELGALATLPRSTRATVAPGSTVWALP
                     AARLQAYLAEHQERGVQFLVNLLGVVAEKMHRDQTRMADMRQNLIRTQKELKRLRELV
                     LETVETPLSAPVHDTLDQLIAHNRRVGYRVEPPASYAARLRVDGGTAEVISLSRTQLT
                     LRAPAPLPDGATWCSGVLDLGGTEIPVSGSVSRSDGKVTIVLDLLIDANAAVLEGYLT
                     RVQLHDILV"
     gene            2711..>2954
                     /locus_tag="VF516_02340"
     CDS             2711..>2954
                     /locus_tag="VF516_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106532.1"
                     /translation="MRIWLLTLAVCACSKDAPAPSRAPVDPAERAAQRTTDGSGAAAP
                     RASPDLVAALVATIPADARVIGSGELLPPAPPPAPPS"
BASE COUNT          398 a         1119 c         1009 g          428 t
ORIGIN      
        1 cctgccgacc gcgatcggcg ccgccgccgg cacgctgctc gccggcggct cgttgtggtc
       61 ggtggtcgcg gcgctcgtcg cggcgccgct ggccgcgctg catccctcgc tcacgaccgg
      121 catggtggtc ggccgggtcg aggccgcgtg gcgccgcccg accctcaccg accgcgagcg
      181 gctcggcgac gacctccaga gcctcgccgg gttccgccgc aacccggtga cccggatcct
      241 gctcgtcgcg atcgcgtcga gcctcggcac ggcgctcggg ttctggttcg cggtcggctg
      301 gctggtgcgg ctgctgtagc tcggcgcgtg gcggccgtca tggcgggccg cgtttcagcg
      361 agtttcatcg cgtcggcgca gcgcgttttt ccgtatgctc gccgcatgat gaagcgacgc
      421 gacgcgctca agacgctggg tgggctcggg gccgcggccg gcatggcgag gttcctcccc
      481 gcctgcggca gtgacggccc gcgcggcatc acgacgtacg tctacctgat gatggagaac
      541 cgcagctacg atcacctgct cggcgcgcgc gcgatggcgc cggagaacaa gggcggcgac
      601 ggcctgccga cgccgacggc gttctccaac cccgacctca acggcatggc cgtcacctcg
      661 ttcacccccg acaaggacag cctgtgcgcg ctcgatccgc cgcacggctg ggacgccgcg
      721 cgggcgcagt ggaaccacgg cgccaacgac ggcttcctgc gcgtccacca gatggctcac
      781 aacaacgatc cgacggcacg ccagccgatg cagtacctgg tccgcagcga cgtgccggtg
      841 tcgtgggcgc tcgccgatgc ctacgcgacc tccgatcgct ggttctgcgc ggtgatgggg
      901 ccgaccttcc ccaaccggtt ctactggcac gcggcgtcgt cggaaggctt gatgtccaac
      961 acgctgccgc ccggcggtct gatggggccg tcgatctacc accggctcga cgccaagggc
     1021 atcccgtgga agtactacta cggcaacatc ccgctggtcg cggcgtacca ggggctcgac
     1081 gtcacccgct tgcagtcgtt cgagcagttc ctggccgacg cccgggccgg caccctgccg
     1141 ccggtcgtgt acatcgaccc ggcgttcgac gccaacgacg accacccgcc ggtccacccg
     1201 atcaacggcc aggagctgat cgcctcggtc tacaacgcgc tcgcgacctc cccgcagtgg
     1261 aagaactgtc tgctgctgat cacctacgac gagaacggcg ggttctacga ccacgtctcg
     1321 ccgccgacga ccgtcgacga gcgcgccgcc cagggcttca accagctcgg cttccgggtc
     1381 ccggcgatcg tcgccgggcc gtatgtcaag cagggctacc tcagctcgct gcagtacgag
     1441 cactgctcgg ccctgcgcga gctcgaggtc gcgttcgggc tcgagccgat caaccagcgc
     1501 atgatggccg cccgcgacct cggcgacttc atcgacagcg agcgcctggc gcggggcgac
     1561 tgggccaagc cgatcacgct cccgacggtc gaccccaccg acatggccca gtggccgatg
     1621 ggcgcgagct gcaagcccgg cgcggacccg ttccccacca cccgtcaccc ggcgatcgag
     1681 tgggcggacg cgcacccggc ggtgctcgcc ggcctcgacc tccggccgca ggtcgcggcg
     1741 taccgccgcg cgatccgcca gtccctggcg cagccgcctg gcgccctggt ccgctagcgc
     1801 ctcggctaca ccaggatgtc gtgcagctgg acccgcgtga ggtagccctc gaggaccgcc
     1861 gcgttggcgt cgatcagcag gtcgagcacg atcgtcacct tgccgtcgct gcggctcacc
     1921 gagcccgaca ccgggatctc ggtcccgccg aggtcgagca ccccggagca ccaggtcgcg
     1981 ccgtcgggca gcggcgccgg cgcgcgcagc gtcagctggg tgcgcgacag gctgatgacc
     2041 tcggcggtgc cgccgtcgac ccggagccgg gccgcgtacg aagccggcgg ctcgacccgg
     2101 tagccgaccc ggcggttgtg ggcgatcagc tgatcgagcg tgtcgtggac cggcgccgac
     2161 agcggcgtct cgacggtctc cagcaccagc tcgcgcagcc gcttgagctc cttctgggtc
     2221 cggatcaggt tctgccgcat gtcggccatc cgggtctgat cgcggtgcat cttctcggcg
     2281 accacgccga gcaggttgac caggaactgc acgccgcgct cctggtgctc ggcgaggtag
     2341 gcctggagcc tggcggcagg cagcgcccac accgtcgagc ccggcgcgac ggtcgcgcgg
     2401 gtcgagcgtg gcagcgtcgc gagcgcgccg agctctccga tgatcgccgg cggcgacagc
     2461 ctgaacacct cgttgggctg ctcgagcacg acctcgccct gggtcagcag gtacatcgcg
     2521 gtcgccggct cgccgatgtc gaacagcggc accgactcgt cgacgatcag ccgggtgaac
     2581 agctcgccga gctccgcgag agcttcatcg ctgaatccgc ggaacagcgg gatcgtgcgc
     2641 agatctgacc cgctgggccc catatggcgc gacgatagcg cgccctgccc tgctgcggta
     2701 ccgtagggcg ttgcgcattt ggctcctcac gctcgcggtg tgcgcgtgca gcaaggacgc
     2761 acctgcgccc tcacgcgcgc cggtggatcc cgccgagcgc gccgcgcagc gtaccacgga
     2821 cggctccggc gccgcagcgc cgcgcgcctc tcccgacctc gtcgccgcgc tggtcgcgac
     2881 gattcccgcc gacgcccggg tgatcgggtc cggcgagctc ctccctcctg ccccgccgcc
     2941 cgcgccaccg tccg
//
LOCUS       DASVOR010000111         3801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_322646,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000111 DASVOR010000000
VERSION     DASVOR010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322646"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(478..2130)
                     /locus_tag="VF516_02345"
     CDS             complement(478..2130)
                     /locus_tag="VF516_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106533.1"
                     /translation="MSTQGAAEPAAGAEADKAAVRATVFTHLAGLVIAPTVSALYERG
                     ALDPLLDARGPVPFDDIVERTHANRGYLRVALRLLRSCGWLALADEPRGAYALTRRGR
                     IVMTLAPPLYAELTSYFLPRALYFEEALFGTADEPVVSLVRELVQRAKDGWRIAVSDD
                     AVTAGVRELVRGHLDGMLIGPAMVALARTGALARIAAGPIDPGELSGNDAMLRCLFDL
                     LAARGWLRRIGVAVELTPAGHYAAQIASAYGVTVSYLPTFAVLPALLFGNPRIPRVDP
                     TGFEILVNRAMNVWGSGGAHRTYFRRLDQVVIDLFNRPIADQPRGICDMGCGDGSLLA
                     HLYRVVADQTERGRMLDRHPLVIVGADFNKVARRATKQTLRQAGIPAFHVIPGDINRP
                     AQLAGDLEALDLDVHELLHIRSFLDHNRPYAGLAGYAAGSRTTRTSGAFAHLGAEIPP
                     DEQEENLVRHLRRWAPYIARYGLLVLELHTLPPELTAAHLDRTPAVAYDGTHGFSDQY
                     LVELPVFLACAREAGLRANDRFAAAFPPSELATVSLNYFTAS"
     gene            complement(2136..3134)
                     /locus_tag="VF516_02350"
     CDS             complement(2136..3134)
                     /locus_tag="VF516_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX8106534.1"
                     /translation="MPRIKTTVVGSYPVLPWMVGHPSRLVLRDAVMAVLKTQELAGLD
                     LVTDGELQRFDPNHPEANGMVDYFVAQLDGIRKAVSPSDFERWRADRAAGYQLPGATV
                     VGKIGPGTLNLPRDCDLTRSLTRSQLKFTCTGPHMLARMLDNRHYPDLPELAMDLAGV
                     LRQQLELVEADVVQLDEAAIAGSPQDARWAAEAINHVLDGVLCEKAVHICFGNYGGQP
                     MLRGFWRDLIPFLNALRADHLVLEFARRGYDEIEVFRDLDPRIGLGIGVIDIKDNEVE
                     SPELIASRLERIVDALGPGRLHYTHPDCGFWMLQRSVVDRKIQALVAGRDLYEGRL"
     gene            complement(3213..>3801)
                     /locus_tag="VF516_02355"
     CDS             complement(3213..>3801)
                     /locus_tag="VF516_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106535.1"
                     /translation="VIVGFGIWLRCIRSQSAVVEVAAHRWTRAVAIEEFNERTEEAWR
                     NEVPYEASFPICHERQRGARQVEDGEDCHMERRDKKDGTFEQVKRCKPKYRSEPVMDS
                     WCRFTARRWKQVDEVKATGTGLSPAWPANVPAGDTPAQLGARRQGRRTETLTLDFRDH
                     GSCDVNDPVWRKYTDGQKLQVEVRSSSGDVVCSSL"
BASE COUNT          559 a         1341 c         1369 g          532 t
ORIGIN      
        1 gtgggacatg cagcccgacg gcagctacgt ccagcgcgcc ggcatgggcg ccaagcacag
       61 ccagcagcag gcgatcgagc gcaccgagcg ccggctcaag gaagcgaccc ggctgcgccg
      121 ccgcaaggtc cagtcgaccg cccgccgcaa gcgctgagcg ggcgtgctcg aaacaatcaa
      181 gcggtgtcga gcatgggtca gggttgcctc gaatgctcga catcgacgaa cgtcgacgat
      241 gacgtcgacc ttgactccag cgttgagctt gacggcagcg aagcggcacg tggacgtctg
      301 accccaccgt cgacctccac cttgaccatc ggtcgcgatt cctcgatgag ggatcgctga
      361 ccatcagaag gtccacgtac aaggtcgaga tcgagtcgac gtttgcggcc gccgttcagg
      421 tcaatgtctt gcgacaacgt caacgtcgac ctcaacgtct tcgcaaggac gtcctcttca
      481 gctcgcggtg aagtagttga ggctgacggt cgcgagctcc gatggcggga acgccgcggc
      541 gaaccggtcg ttggcgcgca gcccggcctc gcgcgcgcag gcgaggaaca ccggcagctc
      601 gacgaggtac tggtcggaga acccgtgggt gccgtcgtag gcgaccgccg gggtgcggtc
      661 gaggtgagcc gccgtcagct cggggggcag ggtatggagc tcgagcacga gcaggccgta
      721 gcgcgcgatg tacggcgccc accgccgcag gtggcggacc aggttctcct cctgctcgtc
      781 gggcgggatc tcggcgccga ggtgggcgaa cgcgccggac gtccgggtcg tgcgcgagcc
      841 ggcggcgtag ccggcgaggc cggcgtacgg ccggttgtga tcgaggaacg accggatgtg
      901 gagcagctcg tggacgtcga ggtcgagcgc ctcgaggtcg ccggcgagct gggccgggcg
      961 gttgatgtcg ccggggatga cgtggaacgc cgggatgccg gcctggcgca gggtctgctt
     1021 ggtcgcgcgc cgcgcgacct tgttgaagtc ggcgccgacg atgaccagcg ggtggcggtc
     1081 gagcatccgg ccgcgctcgg tctggtcggc gaccacgcgg tagaggtgcg cgagcagcga
     1141 gccgtcgccg cagcccatgt cgcagatccc gcgcggctgg tccgcgatcg gccggttgaa
     1201 caggtcgatc accacctggt cgagccgccg gaagtacgtg cggtgcgccc cgccgctgcc
     1261 ccacacgttc atcgcgcggt tgaccaggat ctcgaacccg gtcggatcga cgcgcgggat
     1321 ccgcgggttg ccgaacaaga gcgccggcag caccgcgaac gtcggcaggt acgacacggt
     1381 cacgccgtac gcgctcgcga tctgcgcggc gtagtgaccc gcgggggtga gctcgacggc
     1441 gacgccgatg cggcgcagcc agccgcgcgc cgcgagcagg tcgaacaggc agcgcagcat
     1501 cgcgtcgttg ccggacagct cgccggggtc gatcgggccg gcggcgatcc gggcgagcgc
     1561 gccggtgcgg gcgagcgcga ccatcgccgg gccgatcagc atgccgtcga ggtggccgcg
     1621 caccagctcg cggaccccgg cggtcaccgc gtcgtcggac accgcgatcc gccagccgtc
     1681 cttcgcgcgc tggaccagct cgcggaccag gctgaccacc ggctcgtcgg cggtgccgaa
     1741 cagcgcctct tcgaaataca gcgccctggg caggaagtac gacgtcagct cggcgtacag
     1801 cggcggcgcc agggtcatga cgatccggcc gcgccgggtc agcgcgtacg cgccgcgcgg
     1861 ttcgtccgcc agcgcgagcc agccgcacga ccggagcagc cgcagcgcga cccgcaggta
     1921 gccgcggttg gcgtgggtgc gctcgacgat gtcgtcgaac ggcaccgggc cgcgcgcgtc
     1981 gagcagcggg tcgagcgcgc cgcgctcgta cagcgcgctg accgtcggcg cgatcaccag
     2041 ccccgcgagg tgagtgaaca cggtcgcccg caccgcggcc ttgtcggcct ctgcgccggc
     2101 ggcgggctcg gcggcaccct gcgtgctcat gctcctcata gccggccctc gtagagatcg
     2161 cgcccggcga ccagcgcctg gatcttgcgg tcgacgacgc tgcgctgcag catccagaac
     2221 ccgcagtcgg ggtgcgtgta gtgcagccgg cccgggccga gcgcgtcgac gatccgctcg
     2281 aggcgcgacg cgatcagctc cgggctctcg acctcgttgt ccttgatgtc gatcacgccg
     2341 atccccaggc cgatccgcgg atcgagatcg cggaacacct cgatctcgtc gtagccgcgc
     2401 cgcgcgaact ccagcaccag gtgatcggcc cggagcgcgt tgaggaacgg gatcaggtcg
     2461 cgccagaacc cgcgcagcat cggctgaccg ccgtagttgc cgaagcagat gtggaccgcc
     2521 ttctcgcaca gcacgccgtc gagcacgtgg ttgatcgcct cggccgccca ccgcgcgtcc
     2581 tgcggcgagc cggcgatcgc ggcctcgtcg agctgcacca cgtcggcctc gaccagctcg
     2641 agctgctggc gcagcacgcc ggcgaggtcc atcgcgagct cgggcaggtc cgggtagtgc
     2701 cggttgtcga gcatgcgcgc cagcatgtgc gggccggtgc acgtgaactt gagctgcgac
     2761 cgggtcagcg accgggtcag gtcacagtcg cgcggcaggt tgagcgtccc cgggccgatc
     2821 ttgccgacca cggtcgcgcc cggcagctgg tagcccgccg cgcggtcggc gcgccagcgc
     2881 tcgaagtccg acggcgacac cgccttgcgg atcccgtcga gctgggcgac gaagtagtcg
     2941 accatcccgt tggcctcggg gtggttgggg tcgaaccgct ggagctcgcc gtcggtgacc
     3001 aggtcgagcc cggcgagctc ctgggtcttg agcacggcca tcaccgcgtc gcgcagcacc
     3061 aggcgcgacg ggtgaccgac catccacggc agcaccgggt aactgccgac cacggtggtc
     3121 ttgatgcgcg gcacgccagc caactatatg ccggccgcgg cccgggcgcg ggcgatctcg
     3181 cgccggcctc cgcggcgata tcccacccgg cgctacagcg acgagcagac cacgtcgccc
     3241 gacgacgagc ggacctcgac ctggagcttc tggccgtccg tgtacttgcg ccacaccggg
     3301 tcgttgacgt cacagctgcc gtgatcgcga aagtccagcg tcagggtctc ggtgcggcgg
     3361 ccctggcggc gcgcgccgag ctgcgcggga gtgtcgcccg ccggcacgtt cgccggccac
     3421 gccggggaaa ggccggtgcc ggtcgccttg acctcgtcga cctgcttcca ccgccgcgcg
     3481 gtgaaccggc accagctgtc catcaccggc tcggagcggt acttcggctt gcacctcttg
     3541 acctgctcga acgtgccgtc cttcttgtcg cggcgctcca tgtggcagtc ctcgccgtcc
     3601 tcgacctggc gggccccgcg ctggcgctcg tggcagatcg ggaagctcgc ctcgtacggc
     3661 acctcgttgc gccacgcctc ctcggtgcgc tcgttgaact cctcgatcgc caccgcgcgg
     3721 gtccagcggt gggccgcgac ctcgaccacg gcgctctggc tgcggatgca gcgcaaccag
     3781 atcccgaacc cgacgatcac c
//
LOCUS       DASVOR010000112         3676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_527196,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000112 DASVOR010000000
VERSION     DASVOR010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527196"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VF516_02360"
     CDS             <1..909
                     /locus_tag="VF516_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX8106536.1"
                     /translation="LAAPDVAHRLRRIAVIARVAPADKLAVVNALRRHGEIVAMAGDG
                     INDAPALRAADVGIAVGARSSDVARQTADVVLERADLRSILAAVAEGRAVQDNLRRSI
                     RFQAAGNLGEVLLVLGASLTGRRLIPSLGLLRINLLTDTLPGLALALESSDDGLDALL
                     ARPPIRPGAPILDASDWRRVGRDGAVIAAASAVAAVVGGPLAAFGTIGATQFGYAAAC
                     RAPGQPIRPRFAAMIGGSAALHLLAVASAPARALLRIGGGTPVAIASSGLGMAVPLYL
                     AWRRHADREITRRGSAADHPSKESSA"
     gene            906..1154
                     /locus_tag="VF516_02365"
     CDS             906..1154
                     /locus_tag="VF516_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106537.1"
                     /translation="MRFHLPSFLLGVATGASGAALAPRLRPLVLEIATDCYRVIDAAM
                     LRLARSREDVSDLLAEARARARGRLRKNGAPRPIEVGV"
     gene            1156..1695
                     /locus_tag="VF516_02370"
     CDS             1156..1695
                     /locus_tag="VF516_02370"
                     /inference="COORDINATES: protein motif:HMM:NF040096.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106538.1"
                     /translation="MAWLAVVHHLPDRTRLRSPVLRKSATACTRLADALAAVPGVHEV
                     RVRPYTGSALILHDPGATVEALTEVASGALDGARVLAPGEAPPLPAAVPAFSTLRQKL
                     AEMGREIDREIRRSSDGSVDLGTLATLGLVGAGAAQVAASGELPMPPWFNLAWWAYRT
                     FMTTHNDAPPASPDPPASS"
     gene            complement(1697..2224)
                     /locus_tag="VF516_02375"
     CDS             complement(1697..2224)
                     /locus_tag="VF516_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8106539.1"
                     /translation="MTVQVPVPVLDTPRLVLRGHLVTDLDDSFAMWSSPEVMRITGKP
                     LSREEVWARLLRYIGHWAVHDYGLWSIRERATDRFVGEVGIADFRRDLAFSFDGAPEA
                     AWVLAPWAHGRGYATEAMTAVLAWSAASHPRTVCIIDPDNFASLRVAAKLGYREIGRA
                     DFKGHPILVFERLAG"
     gene            complement(2253..3185)
                     /locus_tag="VF516_02380"
     CDS             complement(2253..3185)
                     /locus_tag="VF516_02380"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8106540.1"
                     /translation="MRNPVLVVAALTPLAALTPLSIIASAGKSAWADDKQDVSKVQIK
                     ATKVAGQVYVIEDATAEFSGGNVGVSVGPDGIALIDDKFAPLAPKLEAALRTVSDKPV
                     RFVINTHYHGDHTDGNAVFGQKSTVIAHENTRKRMAAGAPGEPPAPAAALPVITFEDK
                     LSVHLNGEDIRAIHVPSGHTDTDVVIFFTRSNVVHMGDDFFNGMFPFIDVDGGGSVKG
                     MIANVQKLLEQIPADAKIIPGHGPIATTKELRAFLAMLQGTSAAVEAGIKAHKTADQL
                     KKEKALAKFDSWAHGFFKADDFIDLLYKDLSRKS"
     gene            complement(3242..>3676)
                     /locus_tag="VF516_02385"
     CDS             complement(3242..>3676)
                     /locus_tag="VF516_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106541.1"
                     /translation="RRGNLNTTRLAGELLVGSGGACDVAASVEEVVVLTRLSPGRLVD
                     AVDYVTSPGHAVGSVVTERGVLTRPVPRGGGGRGETWTIASLHAGEPGQIVERGVAAL
                     VRDCPWPLVSAVDLGFAAPITADEARLLDTLDPAGKYRQVQR"
BASE COUNT          467 a         1343 c         1353 g          513 t
ORIGIN      
        1 ctcgcggcac ccgacgtcgc gcaccggctg cggcggatcg cggtgatcgc gcgggtcgcg
       61 ccggccgaca agctcgcggt ggtcaacgcg ctgcgccgcc acggcgagat cgtcgcgatg
      121 gcgggcgacg gcatcaacga cgctcccgcg ctgcgcgccg ccgacgtcgg gatcgcggtc
      181 ggggcgcgat cgagcgacgt cgcccggcag accgccgacg tcgtgctgga gcgcgccgac
      241 ctgcgctcga tcctcgccgc ggtcgccgag ggtcgggccg tgcaggacaa cctgcggcgc
      301 tcgatccggt tccaggcggc gggcaacctc ggcgaggtgc tgctcgtgct cggcgcgtcg
      361 ctcaccggcc ggcggctgat cccgtcgctc ggcctgctgc ggatcaacct cctgaccgac
      421 accttgcccg gcctggcgct cgcgctggag tcgagcgacg acgggctcga tgcgctgctc
      481 gcccgcccgc cgatccggcc cggcgcgccg atcctcgacg cgagcgactg gcggcgggtc
      541 gggcgcgacg gcgccgtgat cgcggcggcc tcggcggtcg cagcggtcgt gggcggcccg
      601 ctcgccgcgt tcggcacgat cggcgcgacc cagttcggct acgctgcggc gtgccgcgcg
      661 cccggtcagc cgatccggcc ccggttcgcg gccatgatcg gaggctcggc cgcgctccac
      721 ctgctcgccg tggccagcgc accggcgcgc gcgctgctgc ggatcggcgg cggcacgccg
      781 gtggcgatcg cgagctccgg cctcgggatg gccgttccgc tgtacctcgc gtggcgccgc
      841 cacgccgatc gcgagatcac ccgccgcggc agcgcggcgg atcacccctc gaaggagagc
      901 tccgcatgag attccacctg ccctcgttcc tgctcggcgt cgcgaccggc gccagcggag
      961 ctgcgctcgc accgcggctc cggccgctgg tcctcgagat cgccaccgac tgctaccgcg
     1021 tgatcgacgc ggcgatgctg cggctcgccc gcagccgcga agacgtctcg gacctgctcg
     1081 ccgaggcccg cgcccgcgcc cgcggccggc tgcgcaagaa cggcgcgccg cggcccatcg
     1141 aggtgggggt gtgacgtggc ctggctcgca gttgtccacc acctccccga tcggacccgg
     1201 ctgcgcagcc cggtgctgcg caagagcgcg acggcatgca cccggctcgc cgatgcgctg
     1261 gccgccgtgc cgggcgtgca cgaggtccgg gtccgcccgt acaccggcag cgcgctgatc
     1321 ctgcatgacc ccggcgcaac ggtcgaggcc ctcaccgagg tcgcgtccgg cgcgctcgac
     1381 ggcgcccggg tgctcgcgcc cggcgaggca cccccgctgc ccgccgcggt gcccgcgttc
     1441 agcacgctcc gccagaagct ggccgagatg ggccgcgaga tcgaccgcga gatccggcgc
     1501 agcagcgacg gctcggtcga tctcggcacg ctcgcgacgc tcggcctcgt cggcgcgggc
     1561 gcggcgcagg tcgcggcgag cggggagctg ccgatgccgc cgtggttcaa cctggcgtgg
     1621 tgggcctacc ggacgttcat gacgacccac aacgacgcgc cgcccgcgtc gcccgacccg
     1681 cctgcgtcgt cgtgactcac ccggcgaggc gctcgaacac caggatcgga tgccccttga
     1741 agtcggcgcg gccgatctcg cgatagccga gcttggccgc gacccgcagc gaggcgaagt
     1801 tgtccgggtc gatgatgcac accgtgcgcg ggtggctcgc ggcgctccac gccagcaccg
     1861 cggtcatcgc ctcggtggcg tagccccggc cgtgcgccca cggcgcgagc acccaggcgg
     1921 cctcgggcgc gccgtcgaac gagaacgcga gatcgcgccg gaagtcggcg atcccgacct
     1981 cgccgacgaa ccggtcggtg gcgcgctcgc ggatgctcca caggccgtag tcgtggaccg
     2041 cccagtggcc gatgtagcgc aacaggcgcg cccagacctc ctcgcggctc agcggcttgc
     2101 cggtgatgcg catgacctcc ggcgagctcc acatcgcgaa cgagtcgtcg agatcggtga
     2161 cgaggtggcc gcgcagcacg aggcgcggcg tgtccagcac ggggacgggg acctgtacgg
     2221 tcatggcgac gcgccatggt agcgcggcga cgtcacgact tgcgtgacag atccttgtac
     2281 agcaggtcga tgaagtcgtc ggccttgaag aacccgtggg cccagctatc gaactttgcc
     2341 agtgccttct ccttcttgag ctggtcggcg gtcttgtggg ccttgatgcc ggcctcgacg
     2401 gccgccgagg tgccctgcag catggcgagg aacgcgcgca gctccttggt cgtggcgatc
     2461 gggccgtggc ccgggatgat cttggcgtcg gccgggatct gctcgagcag cttctggacg
     2521 ttggcgatca tgcccttgac gctgccgccg ccgtcgacgt cgatgaacgg aaacatcccg
     2581 ttgaagaagt cgtcgcccat gtgcacgacg ttggacctgg tgaagaagat caccacgtcg
     2641 gtgtcggtgt ggccgctcgg gacgtggatc gcgcggatgt cctcgccgtt gaggtggacc
     2701 gacagcttgt cctcgaacgt gatcaccggc agcgcggccg ccggggcggg cggctcgccc
     2761 ggggcaccgg ccgccatccg cttgcgcgtg ttctcgtggg cgatcaccgt ggacttctgg
     2821 ccgaacaccg cgttgccgtc ggtgtggtcg ccgtgatagt gggtgttgat cacgaaccgg
     2881 accggcttgt ccgagaccgt gcgcagcgcg gcctcgagct tgggcgcgag cggggcgaac
     2941 ttgtcgtcga tcagcgcgat gccgtcgggg ccgaccgaga ccccgacgtt gccgccgctg
     3001 aactcggcgg tggcatcctc gatcacgtag acctggccgg cgaccttggt cgccttgatc
     3061 tggaccttgc tgacgtcctg cttgtcgtcg gcccacgcgg acttgccggc cgaggcaatg
     3121 atcgacaggg gtgtgagcgc ggcgaggggt gtgagcgcgg cgacgacgag tacgggattg
     3181 cgcatggcgc gcacgctaac acgaggctgc gacatcggcg gcgcggccgc ggtccgccgg
     3241 ctcatcgctg cacctgccgg tacttgcccg cgggatccag ggtgtcgagc agccgcgcct
     3301 cgtcggcggt gatcggcgcg gcgaacccga ggtcgacggc ggacaccagc ggccacgggc
     3361 agtcgcggac gagcgccgcg acgccgcgct ccacgatctg accgggctcg ccggcgtgaa
     3421 ggctcgcgat cgtccaggtc tcaccgcggc cgcccccgcc gcgcggcacc ggccgggtca
     3481 gcacgccgcg ctcggtgacc accgagccga ccgcgtgtcc ggggctggtg acgtagtcga
     3541 ccgcgtcgac cagccgcccg ggcgaaaggc gggtcagcac gacgacctcc tcgaccgacg
     3601 ccgcgacgtc gcacgcgccg cccgagccga ccagcagctc gccggcgagc cgggtcgtgt
     3661 tgaggttgcc gcggcg
//
LOCUS       DASVOR010000113         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_59531,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000113 DASVOR010000000
VERSION     DASVOR010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59531"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..748
                     /locus_tag="VF516_02390"
     CDS             149..748
                     /locus_tag="VF516_02390"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX8106542.1"
                     /translation="MPRTTTPLTLLLILAASLAAPRARADRSPILAALFHNDGEGFTP
                     PAKNQALQALLAQHPALTFLEACGVGDTAEIARQLTRDPKLATTWTGFGWSALHLAAF
                     SGVPGAVQLLLERGAEIDARARSKFKNTPLQAALLGRRDLVDLLLGAGAEINSRSDDG
                     RTAVTEALRGKHAEIADYLRGKGGHGAEITANLEAEPKD"
     gene            complement(1038..>1682)
                     /locus_tag="VF516_02395"
     CDS             complement(1038..>1682)
                     /locus_tag="VF516_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8106543.1"
                     /translation="QSATGQDQDDTVMMPWTTAMKQIVGKDQTWLDDILCSARATDAI
                     APATVQIQELLRQRHHIEPGAEDDFNIRHPEELLQARIKTSRTLRLLLLGLAGIAMVV
                     GGIGVMNVMLASVSQRVAEIGLRCAIGARPAAIQVQFLGEAVALSLLGGALGVGLGEL
                     SGYIFEDKLGWRLAMSPEISAIAVAVSIVAGVVFGLYPAVRAARVDPIVALRTE"
BASE COUNT          263 a          638 c          543 g          238 t
ORIGIN      
        1 cccatcaggc tccggtgcgg cggcaagcgc acggtcaccc cgctatcttg gccgaatcgc
       61 cacggccctg gccataggcc ggaagtcaca cgccggcgcg ggtcgcccgg cacatgcggc
      121 ggcgatcgcg gatcgctagc gtggtcccat gccgagaaca acgaccccgc tcacgctcct
      181 cctgatcctc gccgcctcgc tggcggcgcc ccgcgcgcgc gccgatcgca gcccgatcct
      241 ggcggcgctg ttccacaacg acggcgaggg cttcacgccg ccggccaaga accaggcgct
      301 ccaggcgctg ctcgcccagc atccggcgct caccttcctc gaggcctgcg gggtcggcga
      361 caccgccgag atcgcccgcc agctcacgcg cgatccgaag ctggcgacga cgtggaccgg
      421 gttcggctgg agcgcgctcc acctcgccgc gttctcgggc gtccccggcg cggtccagct
      481 gctgctcgag cgcggcgccg agatcgacgc ccgcgcgcgc agcaagttca agaacacccc
      541 gctccaggcc gcgctgctcg gccgccgcga cctcgtcgac ctcctgctcg gcgccggcgc
      601 cgagatcaac tcgcgcagcg acgacggccg caccgcggtg accgaggcgc tgcgcggcaa
      661 gcacgccgag atcgccgact acctgcgcgg caagggcggg cacggcgcgg agatcaccgc
      721 gaacctcgag gccgagccca aggattgaag atgtgttgat gacagagcaa tgaagcagat
      781 ccggaagcgt cgaccttgtc cttgacgatg accttgaccc gaacctcgac ctcgacggca
      841 acggagacgt gtgaccccac cctcgacgtt gcaccttgat cccgaggtcg acatcgaggt
      901 cgacggtggt gtcgacgtct aagtcgccct cgacctcaac gttcggggtc aatgccgacg
      961 tcaaaccgtc gacgtcaacc accgcttgcg ttccggatgg cccgccttct tttctttcac
     1021 tcgaacctca tgccccgcta ctcggtgcgc agcgcgacga tcggatcgac ccgcgccgcg
     1081 cgcaccgccg ggtacagccc gaacacgacg cccgcgacga tcgagacggc gaccgcgatc
     1141 gcgctgatct cgggcgacat cgcgagccgc caccccagct tgtcctcgaa gatgtaaccg
     1201 ctgagctcgc cgagcccgac gccgagcgcg ccgccgagca gcgacagcgc gaccgcctcg
     1261 ccgaggaact ggacctggat cgccgccggc cgggcgccga tcgcgcagcg caggccgatc
     1321 tcggcgaccc gctgcgacac cgacgccagc atcacgttca tcacgccgat cccgccgacc
     1381 accatcgcga tcccggccag gccgagcagc agcaggcgca gcgtcctgct ggtcttgatc
     1441 cgggcctgga gcagctcctc ggggtggcgg atgttgaagt cgtcctcggc gccgggctcg
     1501 atgtggtggc gctggcgcaa tagctcctgg atctggaccg tcgccggcgc gatcgcgtcg
     1561 gtcgcgcggg ccgagcacag gatgtcgtcg agccaggtct ggtccttgcc gacgatctgc
     1621 ttcatcgccg tggtccacgg catcatcacg gtgtcgtcct ggtcctgccc ggtggccgac
     1681 tg
//
LOCUS       DASVOR010000114         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410336,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000114 DASVOR010000000
VERSION     DASVOR010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410336"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF516_02400"
     CDS             <1..307
                     /locus_tag="VF516_02400"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8106544.1"
                     /translation="TAAVPALDQLVLARDGKPTSARTRLAIEAATARKVLSVQRGAAA
                     LTGYFRSDDPRVREAACVAFTLFTNPGTKQARPALERLLADGDPRVVRAARRALAPR"
     gene            402..>1524
                     /locus_tag="VF516_02405"
     CDS             402..>1524
                     /locus_tag="VF516_02405"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2252 domain-containing protein"
                     /protein_id="HEX8106545.1"
                     /translation="MAVERVTTMTAPPLHRARAEREAFGRRLRDRVRRVDHNIWVAGT
                     RDVLGRLKANEEHRLPGVLPYRHGRMAASPFAFLRGGAAIMAPDLAALPHTGYTVQIC
                     GDAHVRNLGAYASPEGRIVFDVNDFDETCRAPWEWDVKRLAISIALVGREAGTSDRVA
                     RDAVTAMVRSWRETLHELADLPAVELGRYCVHRFAAGGPVGRVLRKAERMTAVQARDE
                     LTAPSHDGKPRFVANPPKLSRVSDAEAQAVLDSLTAYRETLAPNHQQVFDCYLPYDVA
                     FKVAGAGSIGGRNYAVLCVGNGVDDPLTLQVKQALPGCYEALGLVAPDPRVASHQGKR
                     IAEGQHRMQTWTDPLLGWTAIGGVPFYVRQLSDHKASIDP"
BASE COUNT          198 a          564 c          559 g          203 t
ORIGIN      
        1 caccgcggcc gtgcccgcgc tcgaccagct cgtgctcgcc cgcgacggca agccgaccag
       61 cgcccggacc cggctggcga tcgaagcggc gaccgcgagg aaggtgctgt ccgtccagcg
      121 cggcgccgcg gcgctcaccg gctacttccg ctcggacgac ccccgggtgc gcgaggcggc
      181 gtgcgtcgcg ttcacgctgt tcaccaaccc cgggaccaag caggcacggc cggccctcga
      241 gcgcctgctc gccgacggcg acccccgcgt ggtccgcgcc gcccgccgcg cgctcgcgcc
      301 ccgctgatcg tgtgattccc gcggctccgg agtttgcgct atcgtcttga gctcgtcttc
      361 atgtcccgca cgggacgggc gtgggaggat cggaggtcag gatggctgtc gagcgagtga
      421 ctacgatgac cgcgccgccg ctgcaccggg cgcgcgccga gcgcgaggcg tttggccggc
      481 ggctgcgcga ccgggtgcgc cgggtcgacc acaacatatg ggtcgccggg acccgcgatg
      541 tgctgggccg gctcaaggcc aacgaggagc accggctgcc cggcgtcctg ccgtaccgcc
      601 acggccggat ggcggcctcg ccgttcgcgt tcctgcgcgg cggcgcggcg atcatggccc
      661 cggatctggc ggcgctgccg cacaccggct acacggtgca gatctgcggc gacgcccacg
      721 tccgcaacct cggcgcgtac gccagcccag agggccggat cgtgttcgac gtcaacgact
      781 tcgacgagac ctgccgcgcg ccgtgggagt gggacgtcaa gcgcctcgcg atcagcatcg
      841 cgctggtcgg ccgcgaggcc gggacctcgg accgggtcgc gcgcgacgcg gtgacggcga
      901 tggtccggtc gtggcgcgag acgctgcacg agctcgccga tctgccggcg gtcgagctcg
      961 gccgctactg cgtgcaccgg ttcgcggccg gcggtccggt cggccgggtg ctgcgcaagg
     1021 ccgagcggat gaccgcggtc caggcgcgcg acgagctgac cgcgccgtcg catgacggca
     1081 agccccggtt cgtcgccaac ccgccgaagc tgtcgcgcgt gtcggacgcc gaggcccagg
     1141 cggtgctcga ttcgctgacc gcgtaccgcg agaccctggc gcccaaccac cagcaggtgt
     1201 tcgactgcta cctgccgtac gacgtcgcgt tcaaggtcgc cggcgcgggc agcatcggtg
     1261 ggcgcaacta cgccgtgctg tgcgtgggca acggcgtcga cgatccgctg accctgcagg
     1321 tcaagcaggc gctgccgggc tgctacgagg cgctcggcct ggtcgcgccc gatccgcggg
     1381 tcgccagcca ccagggcaag cggatcgccg agggccagca ccggatgcag acctggaccg
     1441 atccgctgct cggctggacg gcgatcggcg gcgtgccgtt ctacgtccgc cagctgtccg
     1501 accacaaggc gtcgatcgat cccg
//
LOCUS       DASVOR010000115        10767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_322656,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000115 DASVOR010000000
VERSION     DASVOR010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10767
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322656"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VF516_02410"
     CDS             <1..915
                     /locus_tag="VF516_02410"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX8106546.1"
                     /translation="ALARQVVEIYCQQIFTDGVYHADPHPGNLIVRPGADGQPPTIVF
                     LDFGAVAEIPGNVRQGMVELLQGALTRDTRRIVGAMKQMGFVARGAHEQTFEQVIEYF
                     HERFQENVSLDSLNLKDIKFDPQKGLESVADLRKMDISLRELSESFHIPKEIIVLERT
                     LLLLMGLCTELDPALNPMTVIRPYLERFVLGDEGDWSQLLVETSKDLVMTVTALPSEI
                     RKFIRLAHAGELQLKFKNFDAPVHLMYRLGHQFIYAAIGIAGAAIAVILEGRGDDPRA
                     AWGWWTARIAGVLLVWSWWTSRALLRKR"
     gene            complement(950..1876)
                     /locus_tag="VF516_02415"
     CDS             complement(950..1876)
                     /locus_tag="VF516_02415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8106547.1"
                     /translation="MTTSSLTIVTGAVPSLRGRQAIGRALAVIAVLIMAATLAISRAG
                     VTGHLGPADLLALRFGVGGLVMLPVLIAGRTRLSRSLAVAAVPLSFAHGWGMAGLSLI
                     GLQFAPASHGGALGPGCIPLWVALLGYALYRHRTSTKQGLGITSISMGVVALLAASYR
                     GLSTAHAVQGDLFFVASSVLAAGYFVYVERHGVPAKLGAALVMVLSGLVVVPAYLVFM
                     PSRIPSAPWREVALQVVVQGPLMSFAYLALHQAVLFIGSKPVSMLLALIPVTTIATGA
                     FIAGDPVSWIEALAVIAVSVGVAIGAAFRARE"
     gene            complement(1866..2663)
                     /locus_tag="VF516_02420"
     CDS             complement(1866..2663)
                     /locus_tag="VF516_02420"
                     /inference="COORDINATES: protein motif:HMM:NF039976.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6065 family protein"
                     /protein_id="HEX8106548.1"
                     /translation="MEKLDDVSTEAPLITFYRMIAEGNHPMRADRSALGVLPTAAFQY
                     CEAISIASAFGWYVFPPIDFHVQWDGTDLIWTHDGGATWSPVTKVHFPGFPDVFDEKA
                     PEDIRGYAPPFLSTVLNGLAPGILQIWTGYMVWTRPDWSLLVRPPANLPRSQGHEPYE
                     GIMETDRWFYPLFSNLRITATDRPIFFDSNKPLLQVQPLRRETYREADLRTAAFKQGL
                     DDFCEADWADYRKSVVDRTRAGVRPGRYASAVRKRAHSGERDDPDDD"
     gene            complement(2708..3010)
                     /locus_tag="VF516_02425"
     CDS             complement(2708..3010)
                     /locus_tag="VF516_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106549.1"
                     /translation="MKTTLGGGAAPKIVLAQQKPVKTSVGGANTPKILLGQQKLVKAS
                     VGGANAPKIVLARQKPVKASVGGGNAPKIVLAQQKPVKATVGGTRIPKITLAQQKK"
     gene            3497..3736
                     /locus_tag="VF516_02430"
     CDS             3497..3736
                     /locus_tag="VF516_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106550.1"
                     /translation="MKWTYTGGGGGASWFHPSATLLSSEGGNGPAPGGTMASGYQGNA
                     GQGGDPQTMPFGAAATPGKAGLMILKVRWCGFQKF"
     gene            3977..5104
                     /locus_tag="VF516_02435"
     CDS             3977..5104
                     /locus_tag="VF516_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106551.1"
                     /translation="MQTFGDHFLQFAQSVDIVPANIYDEALGIVDEHLKAELQASTFV
                     IAIASLSGTTPQLHVDWPKGGWWNHQPIMRHGSYQGQAAFAYATGKPLWIVAKKRQAL
                     SAAGKFEDLLGNVDGAQIPRYARIPRHRGTKTSIILPLRLNDHDLFGVMNIEIPKYLA
                     YSAAWHAELTKIATAIGILYRLTKINELQNRGTLNAKKELTRATFVRVAARRTMFVAS
                     SARADPNVMKILNETLATYAPHFEIVSWTDPQAGNILENIWSKISSCTFAACYFSEPT
                     HAPTSDARPSSRANRQVEYRDNPNVLFEAGMLYALLEIGRAPVKKLLLVREENSPDIP
                     FDLGAEFMILVRRLQNGNVNVSDLKKRVRATVKAILEDERA"
     gene            complement(5181..5429)
                     /locus_tag="VF516_02440"
     CDS             complement(5181..5429)
                     /locus_tag="VF516_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106552.1"
                     /translation="MQLGGVGCNAPSPTCHFICAGGEVWSNVDGAGNRRNVADCAGWT
                     TLGNATAGNDGKTCVTWTAGDCTPIAAGGTLPIFCVQQ"
     gene            5531..6796
                     /locus_tag="VF516_02445"
     CDS             5531..6796
                     /locus_tag="VF516_02445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8106553.1"
                     /translation="MTASYWQLDAGWRDRWVDTAIPRHVDYAIIGAGLAGLATAIRLR
                     QQNPTADIIVLEAERVGYGASGRNAGFLSPLAAPVWLLGAQRSPEQAWGAARINTEVH
                     AIARWLTEHVPDCELEAAKLALQAQSRISDAALREFARAVAFVGLPHQRLESRARSGH
                     RFLEMDAYTVHPYKLVRGLAEHADRQRVRIRERARARSVEALRAGGARVKLDGGGALE
                     AVKVVICTNAYTSAIDSGERVPAFVVHSFMTATAPVDAATLTRDGDFTVEINTTQTYH
                     RMHGGRLIYGGIDKLRAPGGDDFAVPVRERRRLEKAMRDSFPGVTLPIEHAWSGRFHA
                     TATGLPIIRMAADNHAIVFNVGYGGTGAALALVCARLAAAVASHGRFTNVDDTRLLAL
                     VHATRISVRDSMRTLARIARAATMPWLHA"
     gene            complement(6798..7442)
                     /locus_tag="VF516_02450"
     CDS             complement(6798..7442)
                     /locus_tag="VF516_02450"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8106554.1"
                     /translation="MKKAAYVPALGVHVLTAVYDPLIRCWTAATRVRRAMIEALDLRP
                     GMRILELGAGPGRLAIQIKRRHPQVDIVAIDIDARMVARAKRNAVTAGVDVAFRAADM
                     TCVVEGGAYDRVYSTMTFHHLLPEAKDAALSVARSALAPGGSFVVADFGRPRDPLQWA
                     LFSWIQQPLDGFRNTRPHRDGGFERALCGAFADVRSAAVWRTVAGTIEAFVCTP"
     gene            complement(7444..8034)
                     /locus_tag="VF516_02455"
     CDS             complement(7444..8034)
                     /locus_tag="VF516_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106555.1"
                     /translation="MLFDRSCRGRGLAPGLSHAWSAGGALYRALGRIDACFGAGSWAE
                     GLDWLLEASQDRPIAQVQFWGHGEWGGVWIDEELLTTAAFDPAHYLFDRLAKLKARLI
                     PGEALWWFRSCDVFGTTTGHAFARAWTRFFGCRAAGHTRQIMVFQSGLHVLAPGEEPS
                     WPADDGVVDGLWHAAESSMFVPRTVTCLHNTLPEQT"
     gene            complement(8052..8798)
                     /locus_tag="VF516_02460"
     CDS             complement(8052..8798)
                     /locus_tag="VF516_02460"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8106556.1"
                     /translation="MGVVDMIVIALFAMLGLLVAIEHVRPVRAFETVPGWRRKCVAFM
                     PMVIGIAAATPYAMAKVISDVKLLPGERLGIVGGTIFGVVFSELIVYWSHRLHHSVSF
                     LWRWIHQLHHSAERVDALGAAYFHPFEILEGTVVGVLLFNLVLGLAPEAAFLSAAWQA
                     FNGVFQHGNIKTPVWLGYFIQRPEAHAVHHERGVHHFNYANLPLWDIVFGTFRNPVAW
                     QGTAGFYAGASNETLRMLIGRDISGGPPRG"
     gene            8887..9918
                     /locus_tag="VF516_02465"
     CDS             8887..9918
                     /locus_tag="VF516_02465"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8106557.1"
                     /translation="MHLRTIASYADVVRPHLPAHAFEPARSRLLWLPFHISVIVVLAW
                     SITLHRLTPPLWPVASLVIGVSMSAIAFLGHEVLHGAIVRGRVAIRALGGICLLPFTV
                     SPTLWTNWHNRVHHNHCAKVGADPDMYPTLDEYRSQFFARIMADYFGLGGRRCRSLIG
                     LLAGFTGQSQQMLWEARRLGILTPALHRRALVEFMLGVAWWAAIALVVGGVPFVFIYA
                     LPLVVANVVVMMFILTNHSLSSLTPTVNDPLVNSLSVTLPRALEWLSLDFGFHVEHHI
                     FPSMSARHGREVRDALRAHFCERYQSMPITTAVRQLYRTGRVYLDDTTLIDPRTGQTS
                     QALPPRGSP"
     gene            10107..10283
                     /locus_tag="VF516_02470"
     CDS             10107..10283
                     /locus_tag="VF516_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106558.1"
                     /translation="MLDGLKTTGIDLDAVTRHVAQEGVQKFSASCDRLIAAITRRRDA
                     AHAHAHARADIGRR"
     gene            10367..>10767
                     /locus_tag="VF516_02475"
     CDS             10367..>10767
                     /locus_tag="VF516_02475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8106559.1"
                     /translation="MDPAQPQNPTQTGPDRLSPSALPTAPLAAGHKAPDFELRTTPDQ
                     RVALSSFIGRPIVLVFYPADWSPVCGDQLALYNELHDEWQRHRAQLLGISVDNTWCHI
                     AYAKDRRLRFPLLSDFEPKGDVSRRYGAYDY"
BASE COUNT         1927 a         3585 c         3432 g         1823 t
ORIGIN      
        1 gcgctcgccc gccaggtcgt cgagatctac tgccagcaga tcttcaccga cggcgtctac
       61 cacgccgatc cgcaccccgg gaacctgatc gtccggcccg gcgccgacgg tcagccgccg
      121 accatcgtgt tcctcgactt cggcgcggtc gccgagatcc cgggcaacgt ccgccagggc
      181 atggtcgagc tgctgcaggg cgcgctgacc cgtgacaccc ggcggatcgt cggcgcgatg
      241 aagcagatgg ggttcgtcgc gcgcggcgcc cacgagcaga ccttcgagca ggtcatcgag
      301 tacttccacg agcggttcca ggagaacgtc tcgctcgact cgctcaacct caaggacatc
      361 aagttcgacc cccagaaggg cctcgagagc gtcgccgacc tgcgcaagat ggacatctcg
      421 ctgcgcgagc tcagcgagag cttccacatc cccaaggaga tcatcgtcct cgagcgcacc
      481 ctgctcttgc tcatgggcct gtgcaccgag ctcgaccccg cgctcaaccc gatgacggtg
      541 atccggccgt acctcgagcg cttcgtgctc ggcgacgagg gcgactggtc gcagctcctg
      601 gtcgagacct cgaaggacct cgtgatgacg gtgaccgcgc tgccgtccga gatccgcaag
      661 ttcatccgcc tcgcccacgc cggcgagctc cagctcaagt tcaagaactt cgacgcgccg
      721 gtccacctga tgtaccggct cggccaccag ttcatctacg ccgcgatcgg catcgccggc
      781 gccgcgatcg ccgtgatcct cgagggccgc ggtgacgacc cgcgcgccgc gtggggctgg
      841 tggaccgcgc ggatcgccgg cgtcctgctg gtgtggagct ggtggacgtc gcgcgccctg
      901 ctgcgcaagc gctgagccga gcccggcccg gcgccggacg gcagcgatct cactcgcgcg
      961 ctcgaaatgc tgccccgatc gcgaccccca ccgagacggc tatcaccgcc aacgcctcga
     1021 tccacgacac cggatcgcct gcgatgaatg cccctgtcgc gatggtcgtc acggggatca
     1081 gcgcgagcag catcgacacg ggcttgctcc cgatgaacag caccgcttga tgtaaagcaa
     1141 ggtaggcaaa cgacatgagc ggcccttgca cgacgacctg cagcgccact tcacgccatg
     1201 gcgcggacgg aatccgcgat ggcatgaaca ccaggtacgc cgggacgacg acaaggcccg
     1261 agagcaccat gacgagtgcc gcgccgagct tcgccgggac accatgccgt tctacgtaga
     1321 cgaaataacc agcggccagt accgagctcg ccacgaagaa tagatcgcct tgcacagcat
     1381 gcgctgtcga gagcccgcgg taggacgcag cgagcagcgc gacgaccccc atcgagatcg
     1441 aggtgattcc caatccctgc ttggtactcg tgcgatggcg atacagggca taccccagta
     1501 gcgcgaccca caggggtatg catccagggc cgagtgcgcc gccgtggctc gcaggagcga
     1561 actgcagacc gatcagggag agtcccgcca ttccccagcc gtgcgcaaag gacagcggaa
     1621 cagctgcgac cgcaaggctt cggctcagcc tggtccggcc cgcgatgagg accggaagca
     1681 taacgagtcc gccgacgccg aacctcagcg ccaggagatc agccggtccg agatgtcccg
     1741 tgacacctgc gcgcgagatc gccagcgtcg cggccatgat caacaccgcg atcactgcca
     1801 gcgcgcgacc gatcgcctgc cggccgcgaa gcgacggtac ggccccggtg acgatggtga
     1861 gcgagctagt cgtcatccgg gtcgtcgcgc tcgccggaat gggcgcgctt tcgaacggcg
     1921 ctcgcataac gccccgggcg caccccggct cgtgtccgat caacgacgct cttccggtag
     1981 tccgcccagt ccgcttcgca gaaatcgtca agcccttgct tgaacgcagc cgtgcgaagg
     2041 tcggcctccc gataggtctc ccgccggagc ggctgcactt gaagcaacgg cttgttgctg
     2101 tcgaagaaga tcggtcgatc cgtcgccgtg atacgcagat tgctgaacag tggataaaac
     2161 caccgatcgg tttccatgat cccctcgtac ggctcgtgtc cctggctacg cggcaagttc
     2221 gcgggtgggc gtacgaggag gctccagtcg ggacgtgtcc agaccatgta gccggtccag
     2281 atctggagaa tgcccggtgc caggccgttc agaaccgtcg acaggaatgg tggcgcatat
     2341 ccccggatgt cctccggtgc tttctcgtcg aacacgtcgg ggaagccggg gaagtgcacc
     2401 ttggtgacgg gtgaccatgt ggccccgccg tcgtgcgtcc agatcagatc ggttccgtcc
     2461 cattggacgt ggaaatcgat cggtggaaag acgtaccagc cgaacgccga ggcgatgctg
     2521 atcgcctcgc aatactgaaa cgcagcggtc ggtagcacgc ccagtgcgga tcggtcggct
     2581 cgcatcggat gattgccttc agcaatcatt cgatagaatg tgatcagcgg cgcttcggtg
     2641 ctgacgtcat cgagcttttc catgagcgtt ccagaaacga cgttgccctg aagcgtccgc
     2701 gcgcagccta cttcttttgc tgtgcgagcg tgatcttcgg gatgcgcgtg ccgccgacgg
     2761 tcgccttgac gggcttctgc tgcgcgagca cgatcttcgg agcgttcccc ccaccgacgc
     2821 tggccttgac gggcttctgc cgcgcgagca cgatcttcgg agcgttcgca cccccgacgc
     2881 tggccttcac gagcttctgt tgcccgagca aaatcttcgg ggtgttcgca cccccgacgc
     2941 tggtcttcac gggcttctgt tgcgcgagca cgatcttcgg ggcagctccg cctccgagcg
     3001 tggtcttcac gagcttctgt tgcgtgagtg aaatgttctg ggacgtacgg ggggtcatcg
     3061 gcttgcctct cttggttcgc acctcgctct cgcgaagtga gtgcgctggc tgcgctggat
     3121 tcgcgtggca actatcatgg acgagttcgc tgcacaccgc gagacgaaac tcgtcctcac
     3181 cagggagcgc cacatcaacg caatgcgaca cccgtgctgg cgacccctac gtatccgagc
     3241 gagaagatgg cgggagtcag ggatcgccag cagctcgccc ggcgccccgt ttggtgctcc
     3301 cggccgtggg agaagtcact gtcaacgccg cggagcgacc tacccgagtc tcgggggaga
     3361 cgtcgtgaat gtggtttgat tcccggaccc tgaaagacca tcgtcggtga ctcgggccac
     3421 ctgggcggcg ggagcatcgg cgatggctcg agcggcggag gcggctccgg gcaggttcgg
     3481 cggcgggagc ggagccttga aatggacgta caccggagga ggaggcggcg cgtcgtggtt
     3541 tcacccgagc gccacgctcc tcagctccga gggcggtaat gggccggcgc ccggcggcac
     3601 gatggcgtcg ggttatcaag gcaacgccgg ccagggcggc gacccgcaga cgatgccgtt
     3661 tggagccgcg gcgacgcccg gaaaggcggg cctgatgatt ctcaaggtcc gctggtgcgg
     3721 gttccagaaa ttctgaccta accgagatcc gcgcaccgcg tggggctggt ggactgcgcg
     3781 gatcgccggc gtcctgctgg tgtggagctg gtggacgtcg cgcgccctgc tgcgcaagcg
     3841 ctgagctggc tcgcctgttt cacctcgggc acatcgagac atccgggggg ggtgccccga
     3901 gaccacgcct gtttgcccta gagcacaatt acacaagaag gtatgatgta ctagctcact
     3961 tcgggggatc gtgtacatgc aaaccttcgg cgaccatttc ctacaatttg cccagagcgt
     4021 cgacatcgta ccggcaaaca tttacgacga ggccctggga atcgttgacg agcatttgaa
     4081 ggcagaactg caagcctcga ccttcgtgat cgccattgca tcactctcgg ggaccactcc
     4141 gcagttgcac gtagactggc ccaaaggcgg atggtggaat caccaaccga tcatgcgaca
     4201 tggatcctac caggggcaag ctgcatttgc gtatgcaact ggaaagccac tctggattgt
     4261 tgccaagaag aggcaagcgt tgtcagccgc gggcaaattc gaagatctcc tcggcaatgt
     4321 cgacggagca cagattccac gttatgccag gatcccacgt cacagaggaa cgaagacatc
     4381 gatcatcttg ccactccggc taaatgacca tgatttattt ggtgtgatga atatcgaaat
     4441 acccaagtac ctcgcctatt cggccgcgtg gcacgcagag cttaccaaga tcgcgactgc
     4501 cattggaatt ctttatcggt tgaccaagat caatgagctt caaaaccgag gcaccctgaa
     4561 cgccaaaaag gagttgacgc gcgcgacctt cgtccgggtg gccgcgcgtc gcaccatgtt
     4621 cgtggcttcg tcggcacgag ccgacccgaa tgtgatgaag atcctgaacg aaacgttggc
     4681 cacgtacgct ccccatttcg agatcgtgtc gtggacggat cctcaagccg gcaacattct
     4741 cgagaatatc tggtcaaaga tttcgtcgtg tacgtttgct gcgtgctact tttccgagcc
     4801 gacgcacgct cccacgtctg acgcgcgacc gtccagccgt gccaacagac aagtcgaata
     4861 tcgcgacaac ccgaacgttc tgttcgaggc aggaatgctc tatgcgctcc tagaaattgg
     4921 ccgagccccc gtcaagaaac tactgctggt gcgcgaggag aattcaccgg acattccatt
     4981 cgatctcggc gctgaattta tgatcttagt acgacgcctc cagaatggga atgtgaacgt
     5041 gtcggacctg aaaaagcgtg tacgagctac cgtcaaggca attctggagg atgaacgtgc
     5101 gtgatcgcat gtcgaccact gaattcgttc cgagcgcccg cacgaccacg atcgatgcgt
     5161 ggaaagccga gcctcgtccc tcactgctgg acgcagaaga tcggcagcgt gccgcccgcg
     5221 gcgatcgggg tgcaatcccc cgcggtccag gtcacgcagg tcttgccgtc gttgcccgcg
     5281 gtggcgttgc ccagcgtggt ccatcccgcg cagtccgcga cgttccggcg gttgcccgcg
     5341 ccgtcgacgt tgctccacac ctcgccgcct gcgcagatga agtggcaggt cggcgaagga
     5401 gcattgcacc cgacgccgcc cagctgcacg ccgccctcgg tgccagcacg ggcggcgttt
     5461 cgtacgaacg gcgatccacc tctcgcacct cgcgaactac gccgccaccg gttacgatgg
     5521 ccatcgtcga atgacagcga gctactggca actcgacgcc ggctggcgcg accgatgggt
     5581 tgacacggcg attcctcgcc atgtcgacta cgccatcatc ggggctgggc ttgccgggct
     5641 cgccacggcg atccggctgc ggcagcaaaa cccgaccgcg gacatcatcg tgctcgaagc
     5701 ggagcgcgtc ggctatggcg cgagcggacg caacgccggg ttcctttcgc cgctcgctgc
     5761 tccggtgtgg ctgctcggcg cgcagcgttc gccggagcaa gcctggggcg cggcacgcat
     5821 caacaccgaa gtgcacgcga tcgcgcgctg gctcacggag cacgtaccgg actgcgagct
     5881 cgaggcggcg aagctcgccc tgcaggcgca gagcaggatc tccgacgccg cgttgcgtga
     5941 attcgcgagg gcggtggcat tcgtcggcct accgcatcag cgcctcgaaa gtcgagcgcg
     6001 atcgggtcac cggttcctgg agatggatgc gtacacggtc catccgtaca agctcgtccg
     6061 cgggctcgcc gaacatgcag accggcagcg agtgcgtatc cgcgaacgcg cgcgcgcgcg
     6121 cagcgtcgag gcgcttcgtg cagggggcgc gcgggtcaag ctcgacggcg gcggcgcgct
     6181 ggaagccgtg aaggtcgtga tctgcacgaa cgcatacacc tcagcgatcg actccggcga
     6241 gcgcgtgccg gcgttcgtcg tgcacagctt catgaccgcg acggcgcccg tcgacgcggc
     6301 gacgctcacg cgcgacggag acttcacggt cgagatcaac acgacgcaga cgtatcaccg
     6361 catgcacggg ggccggctca tctacggcgg catcgacaag ctgcgcgcgc ccggcggcga
     6421 cgatttcgca gtgccggtcc gggaacgtcg acggctcgag aaggcgatgc gcgacagctt
     6481 ccccggcgtg accctgccga tcgaacatgc ctggtcagga cggttccacg cgacggcgac
     6541 cggtctgccg atcatccgca tggccgccga caaccatgcg atcgtgttca acgttggcta
     6601 cggcggcacg ggtgcggcgc tcgcgctggt gtgtgcgcgc ctggcagccg cggtcgcttc
     6661 gcacggccgg ttcacgaatg tcgatgatac gcgcctgctg gcgctcgtgc acgcgacacg
     6721 gatctcggtt cgcgattcga tgcgaacgct cgcgcggatc gcgcgcgccg ccacgatgcc
     6781 atggcttcac gcctgaatca gggcgtgcag acgaacgcct cgatcgtgcc ggccaccgtg
     6841 cgccagacgg cagcagatcg cacgtcggcg aacgcgccgc atagcgcgcg ctcgaacccg
     6901 ccatcccggt gcgggcgagt attgcgaaag ccgtcgagcg gctgctggat ccagctgaac
     6961 agcgcccact gcaacggatc gcgcggccga ccgaagtctg ccaccacgaa gctgcctccc
     7021 ggggcgagcg cgctgcgcgc caccgagagc gctgcatcct tggcctcggg caacagatga
     7081 tgaaatgtca tcgtcgagta cacgcgatcg tacgcgccac cctcgacaac gcacgtcatg
     7141 tcggcggccc ggaacgcgac atcgacgccc gccgtgacgg cgttgcgctt tgcgcgggcg
     7201 accatgcgcg cgtcgatgtc gatcgcaacg atgtcgacct gcgggtgcct gcgcttgatc
     7261 tggatcgcga gccgcccggg accggcgccg agctcgagaa tgcgcatacc ggggcggaga
     7321 tcgagcgcct cgatcatggc tctgcgcacc cgcgtcgcgg cggtccagca acggatcaag
     7381 ggatcgtaaa ccgcggtcag cacgtgcacg ccgagcgccg gaacgtatgc ggctttcttc
     7441 acgtcacgtc tgctccggaa gcgtgttgtg caggcaggtg acggtgcgcg gcacgaacat
     7501 gctggactcc gcggcgtgcc acagaccatc gacgacaccg tcatcggccg gccagctcgg
     7561 ctcctcacct ggcgcgagca cgtggagccc gctctggaac accatgatct ggcgcgtgtg
     7621 tcccgcagcg cggcacccga agaaacgcgt ccacgcgcgc gcgaacgcgt gcccggtggt
     7681 cgtgccgaag acgtcgcaac tgcggaacca ccacagcgcc tcaccgggta tgagtcgcgc
     7741 cttgagcttc gccagacgat cgaagaggta gtgtgccgga tcgaatgctg ccgtcgtaag
     7801 cagctcctcg tcgatccaca cgccgcccca ctcgccgtgt ccccagaact ggacctgggc
     7861 gatcgggcgg tcttgcgacg cctccaggag ccagtccagg ccttctgccc acgaccccgc
     7921 accgaagcac gcatcgatcc ggccgagcgc gcggtagagg gcgccgccgg ccgaccacgc
     7981 gtgactgagc cccggcgcca gtccgcgtcc gcggcacgag cgatcgaaca gcatgaggcg
     8041 aagcggacga ctcatccgcg cggcggtcct cccgagatgt cacgcccgat caacatgcgc
     8101 agcgtctcgt tcgaggcgcc cgcatagaag ccggcggtac cttgccacgc gaccgggttg
     8161 cggaatgtgc cgaacacgat gtcccagagc gggaggttcg cgtagttgaa gtggtgaacg
     8221 ccgcgctcgt gatgtacggc atgcgcctcg gggcgctgga tgaagtagcc gagccacacc
     8281 ggcgtcttga tgttgccgtg ctggaacacg ccgttgaacg cctgccatgc cgcggacaag
     8341 aatgccgctt ccggcgcgag cccgagcacg aggttgaaca gcaagacgcc cacgaccgtc
     8401 ccttcgagga tctcgaacgg atggaagtac gcagcgccga gtgcgtcgac gcgctcggcg
     8461 ctgtggtgga gctgatgaat ccatcgccac agaaacgaga cgctgtggtg cagtcggtgc
     8521 gaccagtaga cgatgagctc cgagaacacg actccgaaga tggtcccgcc gacgatgccg
     8581 aggcgctcgc ccggcaggag cttcacatcg gagatcacct tcgccattgc gtacggcgtc
     8641 gctgcggcga tgcctatcac catcggcatg aacgcgacgc acttgcgccg ccagcccgga
     8701 acggtctcga aggcgcgtac gggtcggaca tgctcgatgg cgacgagcaa ccccagcatc
     8761 gcgaacagcg cgatgacgat catgtcgacg acccccatgg cgacccgacc agtgtaaccg
     8821 gcggtccgcc gccatggttc ggcaaccgct accctcgggc tcggaaagat gatgtgatgt
     8881 aggtcaatgc acctgcgaac catcgcgagt tacgcggacg ttgtgcgtcc gcacctgccg
     8941 gctcacgcgt tcgagccagc gcggtcgcga ttgctgtggc tccccttcca catatcggtc
     9001 atcgtggtgc tcgcgtggtc gatcacctta catcgactta cacctccact gtggcccgtc
     9061 gcgtcgctcg tcatcggcgt gtcgatgtcg gcgatcgcgt tcctcggtca cgaggttctg
     9121 cacggcgcca tcgtgcgcgg gcgtgttgcc attcgcgcgc tcggcgggat ctgcctctta
     9181 cccttcacgg tgtcaccgac gctgtggacc aactggcaca accgcgtcca tcacaaccac
     9241 tgcgcgaagg tcggcgccga tccggatatg tatccgacgc tcgacgagta ccgctcgcag
     9301 ttcttcgccc gaatcatggc agactacttc ggtctcggcg gtcggcgctg ccggagcttg
     9361 atcggcttgc tggctggctt caccggtcaa agccagcaga tgctctggga ggcccgaaga
     9421 cttggcattc tcacgcccgc gctgcaccgg cgcgccctgg tcgagttcat gctcggcgtg
     9481 gcgtggtggg ccgcgatcgc gctggtcgtc ggtggcgtcc cgttcgtgtt catctacgcg
     9541 ctgccgctcg tcgtcgcgaa cgtcgtcgtg atgatgttca tcctgacgaa tcacagcctc
     9601 agctcgctga ccccgaccgt caacgacccc ctcgtcaact cgctgtccgt cacgctaccg
     9661 cgtgcgctcg agtggctgtc gctcgacttc ggctttcacg tcgagcacca catctttccg
     9721 tcgatgagcg cgcgccacgg ccgtgaggtt cgcgacgcgt tgcgtgctca tttttgcgag
     9781 cgctaccaat cgatgccgat caccacggcg gttcgacagc tctatcgaac gggccgcgtc
     9841 tacctcgatg atacgacgct gatcgatccg cgcaccggac aaacatcgca agcactgcca
     9901 ccgaggggtt cgccatgagg gccgtgctgg ctaccatgcg catccaagcg gaaacctagg
     9961 gggagacccc tatggggtat cttccgctgc aggccgtgct ggtcggagga gacatcggcc
    10021 cgaggctgct gccggtcggc tcgctcgccg ggctctcgcg gcgtcgctcg cgatgccgtg
    10081 gctgctgcgg ccgtgactct cgcgcagtgc tcgatggcct gaagacgacc ggcatcgatc
    10141 tggatgcggt gacccggcac gtggcgcaag aaggcgtaca gaagttctcg gcatcctgcg
    10201 atcggctgat cgccgcgatc actcgccggc gagacgccgc gcacgcgcac gcgcacgcgc
    10261 gcgctgacat cgggcggcgc tgacatccga cggcgctgac atcgggcggc gcggcggtct
    10321 ggctcttgca ccctgatttc atcacatcac tcgaacagag gcagtcatgg acccagcgca
    10381 accccagaat ccgacccaga ccggccccga tcgcctgtcg ccgagcgcgt tgcccactgc
    10441 gccgctcgcg gccggacaca aggcccccga tttcgagctg cgtaccaccc ccgaccagcg
    10501 cgtcgcgctg tcgtccttca tcgggcggcc gatcgtgctg gtattttatc ccgcggactg
    10561 gagcccggtg tgcggggacc agctcgcgct ctacaatgag ctgcacgacg agtggcagag
    10621 gcatcgcgcg cagctgctgg gtatctccgt cgacaacacg tggtgccaca tcgcctatgc
    10681 gaaggatcgc cggctgcgat tcccgctgct atccgacttc gagccgaagg gcgacgtctc
    10741 acggcgctac ggtgcctacg actacga
//
LOCUS       DASVOR010000116         2730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_264067,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000116 DASVOR010000000
VERSION     DASVOR010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2730
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264067"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="VF516_02480"
     CDS             <1..1629
                     /locus_tag="VF516_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106560.1"
                     /translation="SHQAAIREALGPLLALRRAQATRRDDRRYRECVGPDAYRPGETK
                     QQFLTRMGAGTGPVDPGTFPYYVLLVASPEEIPYRMQYQLDVQYAVGRIHFDTVEEYA
                     RYAANVVEAETRPGPAVRTVALWGAENPGDRATRASARHLVAPLADYLERGQADRWQV
                     TRFIGEDATKARLAALLGSDAPSLLVTASHGVGFPAGDPRQRADQGALVCQDWAGPHS
                     GSLTADHYFAGDDIAADADLGGLVGFHFACFGAGTPRYDDFSRKGDAPALVDIAPHAF
                     VARLPQRMLGRARGALACIGHVDRAWASSFLQADPRHAGAVTAQLAVFESALQRLIDG
                     RCVGHAMDHFDLRYAELASDLATRIEDATKYDVAVDDHELAQLWIYANDARDYAVIGD
                     PAVRIGGRSAAAEDRAPEFPVEISAAAPVPAGGPPRPEAAPATLAELAGSTLETLRRA
                     VGDALSLEITTYAGDVATARAAAAGDASGQPRPAAYTCCKIDGGTVLSVPVDERGAVD
                     VELWRIHEAAVAQARQQRADTLRLILSLLPSAAP"
     gene            1715..>2730
                     /locus_tag="VF516_02485"
     CDS             1715..>2730
                     /locus_tag="VF516_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 factor interaction domain-containing
                     protein"
                     /protein_id="HEX8106561.1"
                     /translation="MVADPHQQSTEITQLGSEASTPALPRHPSIVPALTVVYHPVLRR
                     IGDRAILGELAAGREARISRGAPRFAPPRQPRSAPLDHVRISRAPFVLRPHPDRGIVL
                     DPADCRTRITVDGEPVEQPRVITPDQLQRGVILELSDRVALLLHLFAPHAERDTGDLG
                     LVGHSDHIGRVRDDIRRVADLDVPVLIRGETGTGKELVATAIHESGRRARGPLVCVNM
                     AALPPALAAAELFGAARGAYTGGHGQDGYFRRAGGGTLFLDEIGAAPAEIQVMLLRAL
                     ETSEIYPLGAQRPERVDVRIIAATDARLEALIASGAFGAPLLHRLAGYEIHLPPLRER
                     RDDIA"
BASE COUNT          339 a         1113 c          933 g          345 t
ORIGIN      
        1 agccaccagg cggcgatccg cgaggcgctc ggcccactgc tggcgctgcg gcgggcccag
       61 gcgacccgcc gcgacgaccg ccgctaccgc gagtgcgtcg gccccgacgc ctatcgaccc
      121 ggcgagacca agcagcagtt cctcacccgg atgggcgccg gcaccggtcc cgtcgatccg
      181 gggacgtttc cttattacgt gctgctcgtc gcgtcgcccg aggagatccc gtatcgcatg
      241 cagtaccagc tcgacgtcca gtacgccgtc ggccggatcc acttcgacac cgtcgaggag
      301 tatgcgcgct acgccgccaa cgtggtcgag gccgagaccc gacccggccc ggccgtgcgc
      361 acggtggcgc tgtggggggc cgagaacccc ggcgatcgcg ccacccgcgc cagcgcccgc
      421 cacctggtcg ccccgctggc cgactacctg gagcgcggcc aggccgatcg ctggcaggtg
      481 acccggttca tcggcgagga tgccaccaag gcgcgcctgg ccgccctgct cggcagcgac
      541 gcgccgtcgc tgctcgtcac cgccagccac ggcgtcgggt ttcccgccgg cgatccgcgc
      601 cagcgcgcgg atcagggcgc cctggtgtgc caggactggg ccggcccgca ctccgggtcg
      661 ctcaccgccg atcattactt cgccggcgac gacatcgccg ccgacgccga cctcggcggg
      721 ctggtcggat ttcacttcgc gtgcttcggc gccggcaccc cgcgatacga cgacttcagc
      781 cgcaagggcg acgcgcccgc gctggtcgac atcgcgccgc atgcgttcgt cgcccgcctg
      841 ccccagcgca tgctcggccg tgcgcgcggc gcgctcgcct gcatcggcca cgtcgaccgc
      901 gcgtgggcca gctcgttcct ccaggccgat ccgcgccacg ccggcgccgt caccgcgcag
      961 ctcgcggtgt tcgagtccgc gctgcagcgc ctgatcgacg ggcgctgcgt cggccacgcc
     1021 atggaccact tcgacctccg ctacgccgag ctggcgtccg acctggccac gcgcatcgag
     1081 gacgccacca agtacgacgt cgcggtcgac gaccacgagc tggcccagct gtggatctac
     1141 gccaacgacg cgcgcgacta cgccgtgatc ggggaccccg cggttcgcat cggcggccgc
     1201 tcggccgcgg ccgaggaccg cgccccggag ttccccgtcg agatcagcgc cgcggcgccg
     1261 gttcccgccg gcggaccgcc gcgccccgag gcagcgcccg ccacgctcgc cgagctcgcc
     1321 gggtcgaccc tcgagacgct gcgccgcgcg gtcggcgatg cgctgtccct ggagatcacg
     1381 acctacgccg gggatgtcgc gaccgcgcgg gccgccgctg ccggcgatgc ttccggacaa
     1441 ccgcgccctg ccgcctacac gtgctgcaag atcgacggtg gcaccgtgct gagcgtgccg
     1501 gtcgacgagc gcggcgcggt cgacgtcgag ctgtggcgga tccacgaggc cgcggtcgcc
     1561 caggcccgac agcagcgcgc cgacaccttg cggctcatcc tgtcgctgct gccaagcgct
     1621 gcaccgtgac tcccgggagc gccgcgcgat gcgtcccgac cctgaaacct ccggcacccg
     1681 aagcttcccc ggtggtacgg tcccgggtcg caggatggtg gccgatccac accagcagtc
     1741 gaccgagatc acgcagctgg gcagcgaggc ctcgacgccg gcgctgccgc ggcatccgtc
     1801 gatcgtcccc gcgctcaccg tcgtctacca ccccgtgctg cgccggatcg gcgatcgcgc
     1861 catcctcggc gagctcgccg ccgggcgaga ggcgcggatc tcgcgcggcg cgccccggtt
     1921 cgcgccgccg cgccagcccc gcagcgcccc gctcgaccac gtccggatca gccgcgcgcc
     1981 gttcgtgctg cggccgcacc ccgaccgcgg catcgtgctc gacccggcgg actgccggac
     2041 gcggatcacg gtcgatggcg agccggtcga gcagccgcgc gtgatcacgc ccgaccagct
     2101 ccagcgcggc gtgatcctcg agctgtccga ccgcgtggcg ctgctgctgc acctgttcgc
     2161 cccccacgcc gagcgcgaca ccggcgacct cggcctggtc ggccacagcg atcacatcgg
     2221 ccgcgtgcgc gacgacatcc ggcgggtcgc cgacctcgac gtcccggtgc tgatccgcgg
     2281 cgagaccggc accggcaagg agctcgtcgc caccgcgatc cacgagtcgg gccggcgggc
     2341 gcgcggcccg ctggtctgcg tcaacatggc cgcgctgccg cctgcgctcg ccgccgccga
     2401 gctgttcggc gcggcgcgcg gcgcttacac cggcgggcac ggccaggacg gctacttccg
     2461 gcgcgccggc ggcggcacgc tgttcctcga cgagatcggc gccgctccgg ccgagatcca
     2521 ggtcatgctg ctgcgcgcgc tcgagacctc cgagatctac ccgctcggcg cccagcggcc
     2581 cgagcgcgtc gacgtccgca tcatcgccgc caccgacgcc cgcctcgagg cgctgatcgc
     2641 gtccggggcg ttcggcgcgc cgctcctgca ccggctcgcc ggctacgaga tccacctccc
     2701 gccgctgcgc gagcgccgcg acgacatcgc
//
LOCUS       DASVOR010000117         8266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410356,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000117 DASVOR010000000
VERSION     DASVOR010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8266
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410356"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VF516_02490"
     CDS             complement(<1..890)
                     /locus_tag="VF516_02490"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106562.1"
                     /translation="MSCPKCGKDVLDIDVKCGHCGTPLGSTGAQRMLGTVMLGQYELV
                     DVLGQGGMSVVFKGRHNLTNQEVALKILPPELAAHSQVKSRFLEEAKALAALDHPNIV
                     HLYNFGQDGGYFVLAMQFVDGKTWERMIFQAQRLDWVTSCRIAIDVLRALEYAHGRGV
                     IHRDMKPSNVLVRARDGVATVMDFGIAKMTTSTRLTATGQTMGTVRYMSPEQVRGQEV
                     DLRTDIYSLGATLYESLVGDTPFGGSTHFEIMSKQLSDLPRPPSRIGAELPATVEDAV
                     MRSLAKRPEGPARDIERSEMG"
     gene            complement(1048..1959)
                     /locus_tag="VF516_02495"
     CDS             complement(1048..1959)
                     /locus_tag="VF516_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106563.1"
                     /translation="MVLGGVFGLAMLQGGTAEAGHGHFSGSVHVSGGFRGGFRAGASV
                     RFARPAVRPFFRPRVWVGGHWWWNTWWWPRPYYYYYPEYVPSYYGGNYYPVQPGVAAP
                     GAVAVAPRRPSLPTLGFGVFAGGTSVNGMKDASEVGLLGRLRLTPGLLLEAELAKDTF
                     SGAVAPACPPGAFCTDIAVANGDRVDRRIGGSLIWELGPHNSFAPYLLAGGGVQQAKV
                     TSGDFFGSDFTTTQDFGEIGVGLRWALSRSLHLTADIRAGRRQTIDSNQTVVTPVARG
                     TINPPSGGPNNDTEDYTRARIAAVINF"
     gene            2325..3560
                     /locus_tag="VF516_02500"
     CDS             2325..3560
                     /locus_tag="VF516_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106564.1"
                     /translation="MSDPKRGGSRDISELKQRLGLKKATTPTAGATAGRPNGPPVGGV
                     VPPPGLAPPPPPQPVIPNAAEDPFAAMNAMAAVGTVQRAPEIVIVNDGKPVENVGASS
                     HGAAIAKLVAPAVIALIVGVVIGQIAKGANLYNAGLKDARSILGDDKTASTVKNVKKT
                     LSELDGVLDEMKTKNNYRPDTAADKRLRELSARLEVKEAAVFRTAGAIEPELTSQIMS
                     FYAGSAEVKSMLDGHIKAAAADDLALSTGKNATGAAQVKDGENATLAAVGAVRYGALI
                     QAPTDTDRSDFGVKLVELGPPYCGGAQPVTTGKCENNEAPTAYAYRAEPGAAWIKGDL
                     QLQGSESVPAKKLVLLLPDGTRDALIKGSEPGASEVFYVKRLRQLNERTKKLIEEANR
                     LEQRLQAESSKSARFSFFL"
     gene            3762..4643
                     /locus_tag="VF516_02505"
     CDS             3762..4643
                     /locus_tag="VF516_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106565.1"
                     /translation="MRRSRRISIGISILALVGSLALGAPAAVHAGPRPQPAAKKPKRT
                     PMTADHKKALAELFGGFKFGMTKDEVIAVFSKQLDARYDDQIKATTDIAVQDRLRRDK
                     RNEVNRLQQSYISFDAGKPSPWDVSIVEDEFAHNTGESMLERWENQAGKNQRRFFFFF
                     EGKLWKMFVSLDVSILPEDKKNFATFESVMEAKYGPGEVAGGKITWRTDDFEARAIDK
                     LKTYDALALAVEDPRARRQIDAVREAKAPPRREGNTMIQSVIDTGSKDHPSAKADSST
                     VDAVIRAQGSSPPPPNK"
     gene            complement(5325..>8266)
                     /locus_tag="VF516_02510"
     CDS             complement(5325..>8266)
                     /locus_tag="VF516_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106566.1"
                     /translation="SPPGRTFYQRNIEPILQQKCTGNTSGCHSTNPDDPYQFAAGNFD
                     VTSFANVQKRRDVLAPFGAYAYPLLLIMAVPTGALPLFYDTKVPNIDPKVSPSRPLEV
                     QHSGGPILDVNSDAFIALRNWLDNGATENGLKPPTPPQTGNGSCSDSIPSGFSKATYV
                     TPATQASFDAFHNTVQPILTRHGCNAGSCHGAPQSDFYITCGTSDDELAFNFSQAWSF
                     VNTPVEDSQILRIPLAVAAGGRGHTGGDQFASTTDADYLAIRAWAMQAGVLDFAKGDA
                     ARQFFASYVQPILLARGCSFQACHSPAATNDFKLRSGTQGFFSAVALEKNYELLRNEF
                     MAIEFPDARRGRAVSKALISNGDAALGIAHRGGPVLEDPAHPGPCSFDPLKPGASSPL
                     CVIQEWINRERSSPALAGQITDMTTNKPITIVYVERPSGMLDGDRLSFDSFVGGATLR
                     RADTTFGASQQINPATASTAIDSCALGAVDIRTPDVAPDGDHVVFAARKTAGDALQVY
                     LVKLSDAATCVPVTMPAADSNGLKVHNFNPVFSPDGKWIVFASTRGKPSVGATRSRKR
                     FLPQSDLWRVAVNGDTVDQGSYEQITYLSNSEVTPHFMREGRITMTTEKASDGFYQLS
                     GRRINWDRTDYHPLLAQRAISPYASLTDLTQTKPSIGYASATDIREGSDGNFLVILSD
                     LNPDGTPVAPGGGGALGIFNRSIGPFEQGRSDIGYIPALRLVNGTTATGRAGSAASYR
                     APVSLPGGEIMVSYASAATTGNFQIVAVNPHSGAQTTLINTGGGIQLDAVLAYKYPPR
                     TLYDNRRQLVFGGNDAGDPAHAVLHMPDAPMVFTLLTGNLRRGRPVDAFRKAKFLAVY
                     SEGLCPAGACSANTNGIYQNRSLLGRASLADDGSVRVQLPSGTGVVLELQDDKQNSVV
                     QMTEEHQLGPGEQISMGIVQPLFDAVCAGCHGSVTGHEVDVRVTPDALTGASASISAT
                     KAPQSIP"
BASE COUNT         1299 a         2784 c         2935 g         1248 t
ORIGIN      
        1 cccatctcgc ttcgctcgat gtcgcgcgcc ggaccctcgg ggcgcttggc caggctgcgc
       61 atcaccgcgt cctcgaccgt cgccggcagc tccgcgccga tccggctcgg cggccgcggc
      121 agatccgaca gctgcttgct catgatctcg aagtgcgtgc tgccgccgaa cggcgtgtcg
      181 ccgaccagcg actcgtacag cgtggcgccg agcgagtaga tgtcggtccg gaggtcgacc
      241 tcctggccgc ggacctgctc cggcgacatg tagcgcaccg tgcccatggt ctggccggtc
      301 gcggtcagcc gggtcgaggt cgtcatcttc gcgatcccga agtccatcac ggtggcgacc
      361 ccgtcgcgcg cgcgcaccag cacgttcgac ggcttcatgt cgcggtggat cacgccgcgg
      421 ccgtgcgcgt actccagcgc gcgcagcacg tcgatcgcga tccggcacga cgtcacccag
      481 tcgagccgct gggcctggaa gatcatccgc tcccaggtct tgccgtcgac gaactgcatc
      541 gcgagcacga agtagccccc gtcctggccg aagttgtaga ggtggacgat gttggggtga
      601 tcgagcgccg cgagcgcctt ggcctcctcg aggaaccgcg acttgacctg gctgtgcgcc
      661 gccagctcgg gcggcaggat cttgagcgcg acctcctggt tggtcaggtt gtggcggccc
      721 ttgaacacca cgctcatccc gccctggccg agcacgtcga ccagctcgta ctgcccgagc
      781 atcacggtgc cgagcatgcg ctgcgcgccg gtcgagccca gcggggtgcc gcaatggccg
      841 cacttgacgt cgatgtcgag cacgtccttg ccgcacttcg gacagctcat gccgacctat
      901 tcgctcgctc gccgcgcgcg cgatggaaac cggtccgcga ccaccatccc ccgagcgtag
      961 ccgaaaacgc gaccggccgc agagcgcggc cggtggttgc gacgggtcgc tgcgaggaag
     1021 cagccgggtc cgccggatca gccgggatca gaagttgatc accgcggcga tccgggcgcg
     1081 ggtgtagtcc tcggtgtcgt tgttcgggcc gccggacggc gggttgatcg tgccgcgggc
     1141 gaccggggtc accacggtct ggttcgagtc gatcgtctgg cggcggccgg cccggatgtc
     1201 ggccgtcagg tggaggctgc gggacagcgc ccagcgcagc ccgacgccga tctcgccgaa
     1261 gtcctgcgtg gtggtgaagt cggaaccgaa gaagtcaccg ctggtgacct tggcctgctg
     1321 cacgccgccg ccggcgagca ggtacggcgc gaagctgttg tgcgggccga gctcccagat
     1381 cagcgagccg ccgatccggc ggtcgacgcg atcgccgttg gcgacggcga tgtcggtgca
     1441 aaacgccccc gggggacacg ccggtgccac ggcgcccgag aacgtgtcct tggcgagctc
     1501 ggcctccagc agcaggccgg gcgtcaggcg cagccggccg agcagtccga cctccgaggc
     1561 gtccttcatg ccgttgacgc tggtgccacc ggcgaacaca ccgaacccca aggtgggcag
     1621 cgacgggcgg cggggcgcga ccgcgacggc cccgggagcc gccacaccgg gctgcacggg
     1681 gtaataattc ccgccgtaat acgacggcac atactccggg tagtagtagt agtacggccg
     1741 cggccaccac caggtgttcc accaccagtg cccaccgacc cagacccgcg gccggaaaaa
     1801 tgggcggacc gccgggcgag cgaagcgcac cgatgcgccg gcgcggaagc cgccgcggaa
     1861 gccgccgctg acgtggacgc tgccgctgaa gtggccgtgc ccggcctccg cggttccgcc
     1921 ctggagcatc gcgagcccga acacaccgcc gaggaccaat gccgaggagg acacgctacg
     1981 gggcaaacgc atcatggggg ttcgctcttt cggagaagct agccagaagg ccggaaggag
     2041 gaatgaccgc gctaggacac tggagcgcgc cgctcggttt actgcacctg tcaggccagg
     2101 tcggatttcg cgcgatcgcg caagaaacgg cctcgattct gacatcttac gaagtgaatg
     2161 gggaatgaag gttttcttga acgtgtgacc gcattttcac ggccgcgtcg agtgtggtga
     2221 tgcagcgaca gcttcgcgtg gtgtgcaacc ccgccaagtc atgggcatcc ttgacagtag
     2281 ggacgctcgg tccctaaagt cacccttccc gaccggaggt cattttgagc gatccgaagc
     2341 gtggtggtag ccgtgacatc agcgagctca agcagcggct gggactgaag aaagccacca
     2401 ccccgaccgc cggtgccact gcgggacggc ccaacggccc tccggtcggc ggcgtcgtgc
     2461 caccgcccgg gctggccccc ccgccgccgc cccagccggt gatccccaac gcggccgagg
     2521 atccgttcgc cgcgatgaac gcgatggccg cggtcggcac ggtccagcgc gccccggaga
     2581 tcgtcatcgt caacgatggc aagccggtcg agaacgtcgg tgcgtcgtcg cacggcgcgg
     2641 cgatcgccaa gctcgtcgcc ccggcggtga tcgcgctgat cgtcggcgtg gtgatcggcc
     2701 agatcgccaa gggcgccaac ctctacaacg ccggcctcaa ggacgcgcgg tcgatcctcg
     2761 gcgacgacaa gaccgcgtcg acggtcaaga acgtcaagaa gacgctgtcc gagctcgacg
     2821 gcgtgctcga cgagatgaag accaagaaca actaccggcc ggacaccgcg gccgacaagc
     2881 gcctgcgcga gctgtcggcc cggctcgagg tcaaggaggc cgcggtgttc cgcaccgccg
     2941 gcgcgatcga gcccgagctc accagccaga tcatgtcgtt ctacgccggc tcggccgagg
     3001 tcaagagcat gctcgacggc cacatcaagg ccgccgccgc cgacgacctt gcgctgtcga
     3061 ccggcaagaa cgccaccggc gccgcccagg tcaaggacgg cgagaacgcc acgctcgccg
     3121 cggtcggcgc ggtccgctac ggcgcgctga tccaggcgcc gaccgacacc gaccggagcg
     3181 acttcggggt caagctcgtc gagctcggcc cgccgtactg cggcggcgcg cagccggtga
     3241 ccaccggcaa gtgcgagaac aacgaggcgc cgaccgccta cgcctaccgc gccgagcccg
     3301 gcgcggcgtg gatcaagggc gatctccagc tccagggcag cgagagcgtg ccggccaaga
     3361 agctcgtgct gctgctcccc gacggcaccc gcgacgcgct gatcaagggc agcgagcccg
     3421 gcgcctccga ggtgttctac gtcaagcggc tgcgccagct caacgagcgc accaagaagc
     3481 tgatcgagga ggccaaccgg ctcgagcagc ggctgcaggc cgagtccagc aagagcgcgc
     3541 ggttcagctt cttcctctag gagcatgtag acacagcgcc cggagaacgc gtgcacgctc
     3601 ggctcgccgt gggctctgcg cggatgggcg tcgtggccga gcccgccgaa tcacccatcg
     3661 ccaggccggt tgccgggtgg tcggagtgaa tccgcaggcg gatccatacg tttcacacga
     3721 tgcggtcgcg cggtacgatg cggccgctga tggaggcttg tatgcgtcgc tctcgccgga
     3781 tatcgatcgg gatctcgatc ctcgcgctgg tcggctcgct cgcgctcgga gcgcccgcgg
     3841 cggtgcacgc gggtccgcgg ccgcagcccg ccgccaagaa gccgaagcgg accccgatga
     3901 ccgccgacca caagaaggcg ctggccgagc tgttcggcgg cttcaagttc gggatgacca
     3961 aggacgaggt gatcgcggtg ttctcgaagc agctcgacgc gcgctacgac gaccagatca
     4021 aggcgaccac cgacatcgcc gtccaggacc ggctgcgccg cgacaagcgc aacgaggtga
     4081 accggctgca gcagagctac atcagcttcg acgccggcaa gccgtcgccg tgggacgtgt
     4141 cgatcgtcga ggacgagttc gcgcacaaca ccggcgagtc gatgctcgag cgctgggaga
     4201 accaggccgg caagaaccag cgccggttct tcttcttctt cgagggcaag ctgtggaaga
     4261 tgttcgtctc gctcgacgtc tcgatcctgc ccgaggacaa gaagaacttc gcgaccttcg
     4321 agtcggtgat ggaggccaag tacggcccgg gcgaggtcgc gggcggcaag atcacctggc
     4381 gcaccgacga cttcgaggcc cgcgcgatcg acaagctcaa gacctacgac gcgctcgccc
     4441 tcgcggtcga ggatcccagg gcgcgccgcc agatcgacgc cgtgcgcgag gccaaggccc
     4501 cgccgcgccg cgagggcaac acgatgatcc agtcggtgat cgacaccggg agcaaggacc
     4561 acccgagcgc gaaggccgac agctccacgg tcgacgccgt gatccgcgcg cagggcagca
     4621 gcccgccgcc gccaaacaag tgatccgcgg atccggacac ttcgacttgg gtcggcgcgt
     4681 tggcgcggta ccaggggtcg gggagtgtct gttcccgacg aggtcggtgt ctcaatcctt
     4741 gccctggtcc gtttccaacg gccgcccgtc acaccggaca cggagatttc caccatccgg
     4801 cgttgcccat cgagtcagtg cggggtttat gtggccgtgc ccctggagcc gctgcaccag
     4861 gccaccccgt agtcgtggaa gagcccccac gtctcgtaga tgtccttggc gctccacgtg
     4921 gtccaggagc gcttcccgcg tcgcttcttg gggcgttgcc ggctcgcaaa ccgtcggact
     4981 ttcaagtcga cctgccaggc gacgtggctg aggtcccgcc cggagtttcc ccatcggaag
     5041 tagttgaccc atcctcgtac gcgcgggttg acttcgacgg caacggagac gtctgacccc
     5101 acccttgacc ttgcaccttg actccggaag agggtcggca cccatgatca agcccctcga
     5161 cgagcgcggc atcgactgga tgaccagcac cgtcgcgatg tcgacgaagc ggtcaacatc
     5221 gaggtcgacg gtgggggcaa cgtcctaggt cgccgtcaac ctcgacgttc ggggtcaacc
     5281 tcgacgtcaa accgtcaacg ccaccaccgc ttgcatcccg gcggctacgg gatcgactgg
     5341 ggggccttgg tcgccgagat cgaggccgag gcgccggtca gcgcgtcggg ggtgacccgg
     5401 acgtcgacct cgtggccggt caccgagccg tggcagccgg cgcacaccgc gtcgaacagc
     5461 ggctgcacga tgcccatcga gatctgctcg ccggggccga gctggtgctc ctcggtcatc
     5521 tggaccacgc tgttctgctt gtcgtcctgg agctccagca cgacgccggt ccccgacggc
     5581 agctggaccc ggaccgagcc gtcgtcggcg agcgatgcac ggccgagcag cgagcggttc
     5641 tggtagatgc cgttggtgtt ggcgctgcag gcgccggccg ggcacagccc ctcgctgtag
     5701 accgcgagga acttcgcctt gcggaacgcg tcgaccggac ggccgcgccg caggttgccg
     5761 gtcagcagcg tgaacaccat cggcgcgtcc ggcatgtgga gcacggcgtg cgccggatcg
     5821 ccggcgtcgt tgccgccgaa cacgagctgg cggcggttgt cgtacagcgt gcgcggcggg
     5881 tacttgtagg cgagcacggc gtcgagctgg atgccgccgc cggtgttgat cagcgtcgtc
     5941 tgggcgccgc tgtgcgggtt gaccgcgacg atctggaagt tcccggtcgt ggccgcgctc
     6001 gcgtacgaca ccatgatctc gccaccgggc agcgacaccg gggcgcggta gctcgcggcc
     6061 gatccggcgc ggccggtcgc cgtcgtcccg ttgacgaggc gcagcgccgg gatgtacccg
     6121 atgtcgctgc ggccctgctc gaacggtccg atgctgcggt tgaagatgcc gagcgcgccg
     6181 ccgccgcccg gcgccaccgg cgtgccgtcg gggttcaggt cggacaggat gaccaggaag
     6241 ttgccgtcgc tgccctcgcg gatgtcggtc gccgaggcat agccgatcga cggcttggtc
     6301 tgggtcaggt cggtcaggct ggcgtatggc gagatcgcgc gctgcgcgag cagcgggtgg
     6361 tagtcggtgc ggtcccagtt gatgcggcgg ccggacagct ggtagaagcc gtcgctcgcc
     6421 ttctccgtgg tcatcgtgat ccgaccctcg cgcatgaagt gcggcgtgac ctcggagttc
     6481 gacaggtagg tgatctgctc gtacgagccc tggtcgacgg tgtcgccgtt gaccgcgacc
     6541 cgccacaggt ccgactgcgg caggaaccgc ttgcgcgacc gggtcgcgcc gacgctcggc
     6601 ttgccgcggg tcgacgcgaa cacgatccac ttgccgtcgg gcgagaacac cgggttgaag
     6661 ttgtggacct tgagcccgtt gctgtcggcg gcgggcatgg tgaccggcac gcaggtcgca
     6721 gcgtcgctga gcttgaccag gtagacctgg agcgcgtcgc cggcggtctt gcgcgccgcg
     6781 aacacgacgt gatcgccgtc gggcgcgacg tccggcgtgc ggatgtcgac cgccccgagc
     6841 gcgcagctgt cgatcgcggt cgacgcggtg gccgggttga tctgctggct ggcgccgaac
     6901 gtggtgtccg cgcggcgcag cgtggcgccg ccgacgaagc tgtcgaagct cagccggtcg
     6961 ccgtcgagca tgccgctcgg ccgctcgacg tagacgatcg tgatcggctt gttcgtggtc
     7021 atgtcggtga tctggccggc gagcgccggc gagctgcgct cgcggttgat ccactcctgg
     7081 atcacgcaca gcgggctcga cgcgcctggc ttgagcggat cgaagctgca cggcccgggg
     7141 tgggccggat cctcgagcac cgggccgccg cggtgggcga tcccgagcgc ggcgtcgccg
     7201 ttgctgatca gcgccttgga caccgcgcgg ccgcggcgcg cgtcggggaa ctcgatcgcc
     7261 atgaactcgt tcctgagcag ctcgtagttc ttctcgagcg cgaccgccga gaagaacccc
     7321 tgggtgccgc tgcgcagctt gaagtcgttg gtcgcggccg ggctgtggca ggcctggaac
     7381 gagcagccgc gcgccaggag gatcggctgg acgtagctgg cgaagaactg ccgcgcggcg
     7441 tcgcccttgg cgaagtcgag cacgccggcc tgcatcgccc aggcccggat cgcgaggtag
     7501 tcggcgtcgg tcgtgctcgc gaactgatcg ccgccggtgt ggccgcggcc gcccgcggcg
     7561 acggcgagcg ggatgcgcag gatctgcgag tcctcgaccg gcgtgttgac gaacgaccac
     7621 gcctggctga agttgaacgc gagctcgtcg tcgctggtgc cgcacgtgat gtagaagtcc
     7681 gactgcggcg cgccgtggca gctgcccgcg ttgcagccgt gccgggtcag gatcggctgc
     7741 acggtgttgt ggaacgcgtc gaagctggcc tgggtcgccg gcgtgacgta ggtcgccttg
     7801 gagaacccgg acgggataga gtccgagcac gagccgttgc cggtctgcgg cggggtcggc
     7861 ggcttgagcc cgttctcggt cgcgccgttg tcgagccagt tgcgcagcgc gatgaacgcg
     7921 tcggagttga cgtcgaggat cggcccgccg gagtgctgga cctcgagcgg ccgcgacggg
     7981 ctgaccttgg ggtcgatgtt cgggaccttg gtgtcgtaga acagcggcag cgcgccggtc
     8041 ggcaccgcca tgatcagcag cagcgggtag gcgtatgcgc cgaacggcgc cagcacgtcg
     8101 cgccgcttct gcacgttggc gaacgacgtg acgtcgaagt tgccggccgc gaactggtac
     8161 ggatcgtcgg ggttggtcga gtggcagccc gaggtgttgc cggtgcactt ctgctgcagg
     8221 atcggctcga tgttgcgctg gtagaacgtg cggccgggcg gcgagt
//
LOCUS       DASVOR010000118         2213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410372,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000118 DASVOR010000000
VERSION     DASVOR010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2213
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410372"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VF516_02515"
     CDS             complement(<1..930)
                     /locus_tag="VF516_02515"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX8106567.1"
                     /translation="MNVGADTYDSEAPGAPVESVDAFYDQLAPYGTWVDEPDVGRVYI
                     PDTPDYVPYRTGHWQYTNLGFVWVSSEPHAWATTHYGRWAYSSHYGRWYWLPDTTWGP
                     AWVAWRQTGTHYGWAPLAPEVVVRAGWQPPVESWHYCGAEHVLDPNVVRYYEPRERVV
                     VIHREARPMEHYATVSRARVVVGPPAASLRDHRIAIHPVRCEARTVGRWTPAEAHAQV
                     ERARDHRAAFEAQNQHRIDGNARIHAAHVKVIETHPQIKAEVNTRVEIGRRPGEEHGR
                     PGEEHRRPGEEHGRPGEEHGRPGEHAIVPRETKP"
     gene            complement(1140..>2213)
                     /locus_tag="VF516_02520"
     CDS             complement(1140..>2213)
                     /locus_tag="VF516_02520"
                     /inference="COORDINATES: protein motif:HMM:NF022812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HEX8106568.1"
                     /translation="DDLYSAWYAAIRALAVTPVGALPSFATSDAGADLRFNTMTAAYG
                     QLKHNYVLMAGQPYSEFGCEIPDGYVEPAPAAYDALIEYATRLGKIAALVDPGDRTGA
                     AAHAGRVIGTLRVLRAIADDELANRPLTAAEKHWLGMVTELTVDTSEDITGYPPVYSG
                     WYFDLFLEAEADGMRGAGFIADYFTSVEDGIAYAGATSPRLGIFVVDTGGAPRAFVGP
                     VARAYEAHTPLTTRLTDETAAQLTKVDEPWAASYTVADPGAAPGARPDLALHYDRETG
                     NVVVKADHAIGPATIKLLDHHRVPLATLRQPIQAGETVFEFHRKKQVGAVYVVVGAFR
                     DWAVGDSYGEIDDRWGKVEGSDD"
BASE COUNT          276 a          776 c          837 g          324 t
ORIGIN      
        1 cggcttggtc tcgcgcggca cgatcgcgtg ctcgccgggg cggccgtgct cctcgccagg
       61 gcggccgtgc tcctcgccgg gacggcggtg ctcctcgccg gggcggccgt gctcctcgcc
      121 gggacggcgg ccgatctcga cgcgcgtgtt gacctccgcc ttgatctgcg ggtgggtctc
      181 gatcaccttg acgtgcgccg cgtggatccg cgcgttgccg tcgatccgat gctggttctg
      241 ggcctcgaac gccgcgcggt ggtcgcgcgc gcgctcgacc tgcgcgtggg cctcggcggg
      301 cgtccaccgg ccgaccgtgc gcgcctcgca ccgcaccggg tggatcgcga tgcggtgatc
      361 gcgcagcgac gcggccggcg ggccgacgac gacccgcgcg cggctgaccg tcgcgtagtg
      421 ctccatcggc cgggcctcgc ggtggatcac gacgacgcgc tcgcgcggct cgtagtagcg
      481 cacgacgttg ggatcgagca cgtgctcggc gccgcagtag tgccaggact cgaccggcgg
      541 ttgccagccg gcgcggacga ccacctccgg cgcgagcggc gcccagccgt agtgggtgcc
      601 ggtctgccgc cacgcgaccc acgccggtcc ccacgtggtg tcgggcagcc agtaccagcg
      661 gccgtagtgg ctggagtacg cccagcggcc gtagtgcgtc gtcgcccacg cgtgcggctc
      721 ggacgacacc cagacgaagc cgaggttggt gtactgccag tggccggtgc ggtacgggac
      781 gtagtccggc gtatcgggga tgtacacgcg gccgacgtcg ggctcgtcga cccacgtgcc
      841 gtacggcgcg agctggtcgt agaacgcgtc gaccgactcg accggggccc ccggagcctc
      901 ggagtcatag gtatcggcgc cgacgttcac acctacatcg atgccgacgc cgaccgattg
      961 tgcggcggca accgctggga tcgcgccgag acacgctgcc aggcagagct tgccaagagt
     1021 catgggcgca cagtggagca agcaccgggc cacggcaact gtgcgtcgac gcgcgcgcgg
     1081 ggtgaggcgc tggcgccacg gcggggcgat ctgtcccgat cccggcccat cgggcgccgc
     1141 tagtcgtcgc tgccctcgac cttgccccag cggtcatcga tctcgccgta ggagtcgccg
     1201 accgcccagt cgcggaacgc accgaccacg acgtacaccg ctcccacctg tttcttgcgg
     1261 tggaactcga acacggtctc gccggcctgg atcggctggc gcagggtcgc gagcgggacg
     1321 cggtgatgat cgagcagctt gatggtcgcc gggccgatcg cgtggtcggc cttgacgacg
     1381 acgttgccgg tctcgcggtc gtagtgcagc gccaggtcgg gtcgcgctcc cggggcggcg
     1441 ccggggtcgg cgaccgtgta gctcgcggcc cacggctcgt cgaccttcgt gagctgggcc
     1501 gcggtctcgt cggtcagccg ggtggtgagc ggcgtgtggg cctcgtaggc gcgcgcgacc
     1561 gggccgacga acgcgcgcgg cgcgccgccg gtgtcgacca cgaagatgcc gagccgcggg
     1621 gaggtcgcgc cggcgtacgc gatgccgtcc tcgacggacg tgaagtagtc ggcgatgaac
     1681 ccggcgccgc gcatgccgtc ggcctcggcc tcgaggaaca ggtcgaagta ccagccggag
     1741 tagaccggcg ggtagccggt gatgtcctcg ctggtgtcga ccgtcagctc ggtcaccatg
     1801 ccgagccagt gcttctcggc cgcggtcagc ggccggttgg ccagctcgtc gtcggcgatc
     1861 gcgcgcagca cgcgcagcgt gccgatcacg cggccggcgt gcgcggcggc gccggtccgg
     1921 tcgcccggat cgaccagcgc cgcgatcttg cccaggcggg tcgcgtactc gatcagcgcg
     1981 tcgtacgccg cgggcgccgg ctcgacgtag ccgtccggga tctcgcagcc gaactcggag
     2041 tacggctggc cggccatcag gacgtagttg tgcttgagct ggccgtacgc tgcggtcatc
     2101 gtgttgaacc ggaggtcggc gccggcgtcg ctggtcgcga acgacggcag cgcgccgacc
     2161 ggcgtcaccg cgagcgcgcg gatcgcggcg taccacgcgc tgtacagatc gtc
//
LOCUS       DASVOR010000119         3772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_351904,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000119 DASVOR010000000
VERSION     DASVOR010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3772
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351904"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="VF516_02525"
     CDS             <1..313
                     /locus_tag="VF516_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106569.1"
                     /translation="KGSRPGTERARDEQRDEPTPRYGGGPWNVADERGDARFGHARND
                     DADPSELAPHEADNDDDESPSAADPELAAAEAAGEIESGGERAGMRRGEKPRKNKSRE
                     P"
     gene            complement(348..1124)
                     /locus_tag="VF516_02530"
     CDS             complement(348..1124)
                     /locus_tag="VF516_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8106570.1"
                     /translation="MSGKVATWNVNGIRARYTEVLRWIDREEPDVICLQETKASPEHV
                     PEPLLAVAGYHAHWHGHKGYSGVALLLRTAKFGDAPAFVHPHFDLEHRIVIATAGDVV
                     FGSVYVPNGGKDLPAKMTFLRELVAWAGQLQDEHRHLVLCGDFNVARTDIDVHPMLRK
                     PMIGQSSDERALIEALFGYGLVDVARRVDPDNDRLFTWWAPWRNQRQRNIGWRLDYVA
                     AADALVDETVHCATYRDVGTSDHAPVIAHLRDAPRTAAAP"
     gene            complement(1170..1886)
                     /locus_tag="VF516_02535"
     CDS             complement(1170..1886)
                     /locus_tag="VF516_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106571.1"
                     /translation="MAPLLVNGELIGPVTLVRNGTKTIGVADAELLRPHVGAALTVAT
                     MLDGSAVAAVGHWGLSRKSELGLIDLADPVEAGHDVRPIELGAVCGSLDTRGAPAALV
                     GIVRDGARFVRTHVPVVLDKDEGGDAADAVLHLASPLEDAHVGMRVEGSTLWAWFPPN
                     PALGRSGEVLVLGLAQAYRGFSKPRPHPVIAEIVGLDDLGRALLVAHKAEEREPELRQ
                     VAGEIVAGGAPGGRPGPKKR"
     gene            2099..2728
                     /locus_tag="VF516_02540"
     CDS             2099..2728
                     /locus_tag="VF516_02540"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8106572.1"
                     /translation="MTPQHQPTLLGVAPVVAHAPLTAVRRYRIVVGIDLSEYADIVIE
                     HALDQAARHDRPELHFLTVREKRRPSGEEVKRALWERVYPALEAFNRHGSDWRAQLHV
                     LRGKPEVEIAELAADLRADLLVIGQFGLHTPRARDNNLPNRVLQHAVCPTLVVSMPGE
                     VDAKQCPLCVAVREQTDGQSWFCPEHAGDRGRDHFVSPMTTWSGGSLMW"
     gene            complement(2738..3232)
                     /locus_tag="VF516_02545"
     CDS             complement(2738..3232)
                     /locus_tag="VF516_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106573.1"
                     /translation="MIGFQLEVPSEVELLEGPTLKCRELLQGGRTRGELEIGLFAAAL
                     VIDRDGILSEKACEVLAGEATLRRGPTAVAVALPGASGFRADAVINATLPYRFAFAIA
                     PPDLGVDGGISIVVRCASPDWPAADHMLRSLRILTRTGRVATQVEVDDGPLLPVVTPL
                     PKRS"
BASE COUNT          560 a         1331 c         1385 g          496 t
ORIGIN      
        1 caagggcagc cgccccggca ccgagcgcgc ccgcgacgag cagcgcgacg agcccacgcc
       61 gcgctacggc ggcgggccgt ggaacgtcgc cgacgagcgc ggcgatgcgc ggttcggaca
      121 cgcccgcaac gacgacgccg acccctcgga gctcgctccg cacgaggccg acaacgacga
      181 cgatgaatcg ccgagcgcgg cggatccgga gctcgccgcg gcggaagccg cgggcgagat
      241 cgagagcggc ggcgagcgcg ccggcatgcg ccgcggcgag aaaccgcgca agaacaagtc
      301 gcgcgagccg tgatccgtcc tgcgggcctg cgcgacaacg gcgcaggtca cggcgccgcc
      361 gcggtccgcg gcgcgtcgcg caggtgggcg atcaccggcg cgtggtcgct ggtgccgacg
      421 tcgcggtagg tcgcgcagtg cacggtctcg tcgaccagcg cgtcggcggc cgcgacgtaa
      481 tcgagccgcc agccgatgtt gcgctggcgc tggttgcgcc acggcgccca ccaggtgaac
      541 agccggtcgt tgtcggggtc gacccggcgc gcgacgtcga ccaggccgta gccgaacagc
      601 gcctcgatca gcgcgcgctc gtcgctggac tggccgatca tcggcttgcg cagcatcggg
      661 tggacgtcga tgtcggtgcg cgcgacgttg aagtcgccgc acaggacgag gtgccggtgc
      721 tcgtcctgga gctggccggc ccacgcgacc agctcgcgca ggaaggtcat cttggccggc
      781 aggtccttgc cgccgttggg cacgtagacc gagccgaaca cgacgtcgcc ggcggtggcg
      841 atcacgatgc ggtgctcgag gtcgaagtgg gggtggacga acgccggggc gtcgccgaac
      901 ttcgcggtgc gcagcagcag cgcgacgccg gagtagccct tgtggccgtg ccagtgcgca
      961 tggtagccgg cgaccgcgag cagcggctcg gggacgtgct cgggcgacgc cttggtctcc
     1021 tgcaggcaga tcacgtcggg ctcctcgcgg tcgatccagc gcaggacctc ggtgtagcgg
     1081 gcgcggatgc cgttgacgtt ccaggtcgcg accttcccgg acagcccagg agcacgaacg
     1141 gccgcggatc ggcggcgcgg ccgcgccggt cagcgcttct tggggcccgg ccgcccgccc
     1201 ggcgcgccac cggcgacgat ctcgccggcg acctggcgca gctcgggctc gcgctcctcg
     1261 gccttgtggg cgaccagcag cgcgcggccg aggtcgtcga gcccgacgat ctcggcgatc
     1321 accggatgcg ggcgcggctt ggagaacccg cggtaggcct gggcgaggcc gagcacgagg
     1381 acctcgccgc tgcggccgag cgcggggttg ggcgggaacc acgcccacag cgtcgagccc
     1441 tcgacccgca tgccgacgtg ggcgtcctcg agcgggctgg cgaggtggag cacggcgtcg
     1501 gctgcgtcgc cgccctcgtc cttgtcgagc acgaccggga cgtgggtgcg gacgaaccgc
     1561 gccccgtcgc gcacgatgcc gaccagcgcg gccggggcgc cgcgggtgtc gaggctgccg
     1621 cacaccgcgc cgagctcgat cggccggacg tcgtggccgg cctcgaccgg gtcggccaga
     1681 tcgatcaggc cgagctcgga cttgcggctg aggccccagt ggccgaccgc ggcgaccgcc
     1741 gagccgtcga gcatggtcgc gacggtgagc gcggcgccga catgcgggcg cagcagctcg
     1801 gcgtcggcca cgccgatggt cttggtgcca tttcgcacca gcgtgaccgg gccgatcagc
     1861 tcaccgttga ccaggagcgg cgccaccgag cgaagctcac gtgcatcacg atcgcggtcg
     1921 cgcaccctgc aagggttgca tgaccaccgc cgcgttctca acagatgtcg ccgtcaccgt
     1981 gcgccggcgc tcgatccaat cgctccggcc ggccctccgg acggccctcc ggccagccgg
     2041 tctcgtgcgg cctcggcaaa ccaccgatcg cggcatgccg gatgcaactt cacgcgacat
     2101 gaccccacaa catcaaccga ccctgctcgg ggtggcgccg gtcgtggcgc acgcgccgct
     2161 gaccgccgtg cggcgatacc ggatcgtcgt cgggatcgat ctgtcggagt acgcggacat
     2221 cgtgatcgag cacgcgctcg atcaggcggc gcggcacgat cgtccggagc tgcacttcct
     2281 gaccgtccgc gagaagcgcc ggccgagcgg cgaggaggtc aagcgcgcgc tgtgggagcg
     2341 ggtgtacccc gcgctggagg cgttcaaccg ccacggcagc gactggcggg cgcagctcca
     2401 tgtcctgcgc ggcaagcccg aggtcgagat cgccgagctc gcggccgacc tgcgcgccga
     2461 tctgctcgtg atcgggcagt tcgggctgca cacgccccgg gcccgcgaca acaacctgcc
     2521 caaccgggtg ctgcagcacg cggtgtgccc gacgctggtc gtcagcatgc ccggcgaggt
     2581 cgacgcgaag cagtgcccgc tgtgcgtggc ggtccgcgag cagaccgacg gccagagctg
     2641 gttctgcccc gagcacgccg gcgaccgcgg ccgcgatcac ttcgtgtcgc cgatgacgac
     2701 gtggtcgggc ggcagcctga tgtggtagcc gccggcgtca gctgcgcttc ggcagcggcg
     2761 tgaccaccgg cagcagcggc ccgtcgtcga cctcgacctg ggtcgcgacc cggccggtgc
     2821 gggtcaggat gcgcagcgac cgcagcatgt ggtcggcggc cggccagtcc gggctggcgc
     2881 agcgaacgac gatcgagatg ccgccgtcga ccccgagatc gggcggcgcg atcgcgaacg
     2941 caaagcggta gggcagcgtc gcgttgatga ccgcgtcggc gcgaaagccg ctggcgccgg
     3001 gcaacgcgac cgcgaccgcc gtgggtcccc ggcgcagcgt ggcctcgccg gcgagcacct
     3061 cgcaggcctt ctcggacagg atgccgtcgc gatcgatcac cagcgccgcc gcgaacaggc
     3121 cgatctccag ctcgcctcgc gtccgcccgc cctgcagcag ttcgcgacac ttcagcgtcg
     3181 ggccttcgag gagctcgacc tcactgggca cctcgagctg aaacccaatc atgggccgac
     3241 cccgcggcgg ccggcgatag gtcgtgggct gtgtcatgac ctagacacct gattctattg
     3301 cgggagtcac caagagcgca ccgggaaaga tcccgagcga tctcagtaag atctgcctac
     3361 ctacatgtcg ttgacgctcg atccgagcgc tggcgcacgg tcacggaggg cgctccggag
     3421 aaaagcgcgc tcgtggccgt tcccgtcgcg ctcgacgatg acgtcgacct tgactcgagc
     3481 gttgagcttg acggcagcga tgcgatacgt ggacgtctga ccccaccgtc gacctcgacc
     3541 tcgacctcgc cgtcgtcgcg ctccgtagag gctcgcagca caggatcggt ctaggtccaa
     3601 cgtcgatggt gggctcaacg tcgtacgtcg ccgtcgacct cgacgctctg ggtcaacgat
     3661 caggtcgacg tcgacgatca cgaacgccat atcgacaggg cgtcaggggt cgtgcggacg
     3721 ggctcggtta tgctgtcggg gatggccggt ggcgacgacg agacggcgag cg
//
LOCUS       DASVOR010000120         1719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_322700,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000120 DASVOR010000000
VERSION     DASVOR010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322700"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="VF516_02550"
     CDS             <1..1640
                     /locus_tag="VF516_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106574.1"
                     /translation="LAVLVAVSCAYPPAARAAPTPAAPPAAKPDEAKPGDATPGDAKP
                     DELKPEEKTSRGAVTVGGRRIDYTAIAGTLVVHPKGAEDNPPEPPPGERPDGGKGERP
                     VPPAAAMSYVAYFQTGADSTRPVMFLYNGGPGSATVWLHMGAFGPRRVVTAEDSHTPA
                     APYRLIDNAYSLLDVSDLVFVDAPGTGFGKMVGKDKEKAFWGVDPDADAFADFITQFL
                     SKYGRWNSPKYLFGESYGTTRSAVLARVLLTQKYVDLNGVILLAQILCFDADVDGPEG
                     NPGVDLPYQLALPTYAATAWYHKKLPDPPASLEALIADVEKFAMTEYAAALAAGNTLE
                     SGQRKAVLEKLHRYTGLPVAYLDKANLRVAGGEFEKNLQDDTNLTTGRLDTRFSGPTM
                     DPLSKSADYDPQSASIGAAYVSAYNDYARRTLGVPADKRYTVFAQTYKSWSWKHGDGF
                     GLPTTTNVMPDLAAAMKYNPNLVVQLEAGYFDLATPFYEGVYEFQHLPLPPRQQANLE
                     MKFYPSGHMIYANEPSLRALHDNVASLVRRTAGAKSK"
BASE COUNT          278 a          631 c          593 g          217 t
ORIGIN      
        1 ctctcgccgt gctggtcgcc gtctcgtgcg cctatccacc cgccgcccgg gcggcgccca
       61 cgccggccgc gccgccagcg gccaagcccg acgaggccaa gccgggcgac gccacgccgg
      121 gcgacgccaa gcccgacgag ctcaagccgg aggagaagac cagtcgcggc gcggtgaccg
      181 tcggcggccg ccgcatcgac tacaccgcga tcgccggcac gctggtcgtc caccccaagg
      241 gcgccgagga caacccgccc gagccgccgc cgggcgagcg gcccgacggt ggcaagggcg
      301 agcggccggt cccgcccgcc gcggcgatgt cgtacgtcgc gtacttccag accggcgcgg
      361 acagcacgcg gccggtgatg ttcctctaca acggcggccc gggctcggcg acggtgtggc
      421 tgcacatggg cgcgttcggc ccgcgccgcg tggtcaccgc cgaggactcg cacacgccgg
      481 ccgcgccgta ccggctgatc gacaacgcgt acagcctgct cgacgtcagc gacctggtgt
      541 tcgtcgacgc gcccggcacc gggttcggca agatggtggg caaggacaag gagaaggcgt
      601 tctggggcgt cgaccccgac gcggacgcgt tcgccgactt catcacccag ttcctgtcga
      661 agtacgggcg atggaactcg ccgaagtacc tgttcggcga gagctacggc acgacgcgct
      721 cggcggtgct cgcgcgggtg ctgctgaccc agaagtacgt cgacctcaac ggcgtgatcc
      781 tgctggcgca gatcctgtgc ttcgacgccg acgtcgatgg ccccgagggc aacccgggcg
      841 tcgatctgcc gtaccagctg gcgctgccga cctacgccgc gaccgcctgg taccacaaga
      901 agctgcccga tccgccggcg tcgctcgagg cgctgatcgc cgacgtcgag aagttcgcga
      961 tgaccgagta cgccgccgcg ctggcggccg gcaacacgct cgagtcgggg cagcgcaagg
     1021 cggtgctgga gaagctccac cgctacaccg gcctgccggt cgcgtacctc gacaaggcca
     1081 acctgcgggt cgccggcggc gagttcgaga agaacctgca ggacgacacc aacctgacca
     1141 ccggccggct cgacacccgg ttctcgggtc cgacgatgga cccgctcagc aagtccgccg
     1201 actacgatcc gcagtcggcg tcgatcggcg cggcgtacgt gtcggcatac aacgactacg
     1261 cgcgcaggac gctcggcgtg ccggccgaca agcggtacac ggtgttcgcc cagacctaca
     1321 agtcctggag ctggaagcac ggcgacggct tcgggctgcc gaccacgacc aacgtgatgc
     1381 cggatctcgc ggccgcgatg aagtacaacc cgaacctcgt ggtccagctc gaggccggct
     1441 acttcgatct cgcgacgccg ttctacgagg gcgtctacga gttccagcac ctgccgctgc
     1501 cgcccaggca gcaggccaac ctcgagatga agttctaccc gtccggccac atgatctacg
     1561 ccaacgagcc gtcgctgcgc gcgctgcacg acaacgtcgc gtcgctggtc cggcgcacgg
     1621 cgggcgcgaa gtcgaagtga cggcgccgcg tcacgggcag ctgccgaggc cggccgcgcc
     1681 ggtcgccggc aggtcggcgc tgcggccggg cagcggcag
//
LOCUS       DASVOR010000121         2084 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_1254,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000121 DASVOR010000000
VERSION     DASVOR010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF516_02555"
     CDS             complement(<1..209)
                     /locus_tag="VF516_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106575.1"
                     /translation="MSTLRRIALIWLAAGLGTAHADGLDGERFVPSVGAEGTFVDEHP
                     AVPSHLGWSAGLFFNYADDQIVLRDA"
     gene            808..>2084
                     /locus_tag="VF516_02560"
     CDS             808..>2084
                     /locus_tag="VF516_02560"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEX8106576.1"
                     /translation="MTSDACLAVVGMALRFPGAADPDAFWRNLAGGVESITRLPAPAG
                     AAHVPACGVLADPDCFDAELFGYAPRDALIIDPQHRVFLECAWEALEHAGYDPTRYPG
                     PIGIYAGSSQTGYAELLRARRDAELLAGIGDFALRVGSGLDFLTSRVAYKLGLRGPAV
                     TVQTACSTSLVAIHLACQALLAGDCEIALAGGVTVHVPACPGEYSADGILSADGRCRA
                     FDAAAGGTVGGDGAGVVVLKRLDAALADGDAIHAVVLASAINNDGAGKAGYTAPSVTG
                     QARAIATALQLADVPARTISLVEAHGTGTPLGDPIEIAALTAVYARDTDDRGWCGIGS
                     VKSNIGHLSQPSGIVSVIKTVLAMEHGLIPPTVNYETPNPAIDFADTPFYVANTLTKW
                     DTDGAPRRAGVSSFGIGGTNAHVVLEEAPAAYRT"
BASE COUNT          340 a          735 c          707 g          302 t
ORIGIN      
        1 gcgtcgcgca gcacgatctg atcgtcggcg tagttgaaga acagcccggc gctccagccc
       61 aggtgcgacg ggaccgccgg gtgctcgtcg acgaacgtgc cctcggcccc gaccgatggc
      121 acgaagcgct cgccgtcgag cccgtcggca tgcgccgtgc cgagccccgc cgcgagccag
      181 atcagcgcga tgcgacgcag ggttgacatc gccggcgatg atagcgccgt cgccccacgc
      241 gcactccccg cggagggcgt gcaaattttg aacttcgagc gcagcgagac ggggggtggg
      301 gcccccgacg gctccgccgg cggggcgggg cgggggcaga gcccccccgt gggtcgacgg
      361 aatgctgcaa ccgcgcttac cagccgtgac aacccgtagg gggtgagctc acagctcctc
      421 acggttcacc ctacatccgt atccgcacgc agcgcaatga gacgctgcgc acagcggcaa
      481 ataccaggtg cccgtgcgct cgaatcgctt gaagcgtatt ccggatgact tcgagtggtc
      541 attcaggaca gctgccacag caatatatat gcgaatgacg cactgcaccc ggatgtgact
      601 tccatgcaac aagccattgt gctccgagtc gcaatcaaaa catgatcccc caatattcag
      661 caaaatcgac tgtgcaaata tcgccatcga gtgcgaacgc agagaatcaa gatattgtgt
      721 caggactgca gcgttgtgct acatcgtact gcagatcttt tgcttgaccg gagactcgac
      781 acgtagatat ggtccgcgga cgcgccgatg accagcgacg cctgcctcgc ggtggtcggg
      841 atggcgctcc ggttccccgg cgccgcggat cccgacgcgt tctggcgaaa tctcgccggc
      901 ggggtcgagt cgatcacccg gctgccggcg cccgccggag ccgcgcacgt ccccgcgtgc
      961 ggcgtgctcg ccgacccgga ctgcttcgac gccgagctgt tcgggtacgc gccgcgcgac
     1021 gcgctgatca tcgaccccca gcaccgggtg ttcctcgaat gcgcatggga ggcgctggag
     1081 cacgccggct acgatccgac gcgctatccc ggaccgatcg gcatctacgc cgggtccagc
     1141 cagaccggct acgccgagct gctgcgcgct cgtcgcgacg ccgagttgct cgccgggatc
     1201 ggcgacttcg cgctgcgggt cggttccggc ctcgacttcc tgaccagccg ggtcgcgtac
     1261 aagctcggct tgcggggacc cgcggtcacc gtgcagaccg cctgctcgac ctcgctggtc
     1321 gcgatccacc tggcgtgcca ggcgctgctc gccggggact gcgagatcgc gctcgccggc
     1381 ggcgtcaccg tccacgtacc ggcgtgcccc ggggagtact cggcggacgg catcctgtcg
     1441 gccgacggcc gctgccgggc gttcgacgcg gcggcgggcg gcacggtcgg cggagacggc
     1501 gccggcgtgg tcgtgctcaa gcggctggac gcggcgctcg ccgacggcga cgcgatccac
     1561 gcggtcgtgc tcgcgtccgc gatcaacaac gacggcgccg gcaaggccgg ctacaccgcg
     1621 ccgagcgtca ccgggcaagc cagggcgatc gcgacggcgc tgcagctggc ggacgtcccg
     1681 gcgcgcacga tctcgttggt cgaggcgcac ggcaccggca ccccgctggg tgacccgatc
     1741 gagatcgccg ccctcaccgc cgtctacgcc agggacaccg acgaccgcgg ctggtgcggc
     1801 atcggttcgg tgaagtccaa catcggccac ctgagccagc cctccggcat cgtcagcgtg
     1861 atcaagacgg tgctggccat ggagcacggc ctgatcccgc cgaccgtcaa ctacgagacg
     1921 ccgaacccgg cgatcgactt cgccgacacc ccgttctacg tggcgaacac gctgaccaag
     1981 tgggacaccg acggcgcacc gcgccgggcc ggggtgagtt cgttcggcat cggcggcacc
     2041 aacgcgcacg tggtgctgga ggaggcgccg gcggcgtacc ggac
//
LOCUS       DASVOR010000122         4063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_468835,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000122 DASVOR010000000
VERSION     DASVOR010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_468835"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1532
                     /locus_tag="VF516_02565"
     CDS             153..1532
                     /locus_tag="VF516_02565"
                     /inference="COORDINATES: protein motif:HMM:NF014281.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8106577.1"
                     /translation="MAESGSSVSVSAARWLVAVIASVVLCFTVASAVGEYLESAIGER
                     ANDITADAMPSVQLLTTVQREVQQIDLDMDRYAAAPVEQRPEHRARIIATRKDVAASF
                     ASYAALPSLADEPLLQADLRTSLADLDDAVADYFSDGQPRSLARVHVQLDLAEEAIAR
                     SVSFNAAQGQRLGTEIQHIRSETRGIVELLDVASVVLAIGACYLAVRQLRRAARDQKA
                     EREARERRAAELAVQNEALGQFAGRVAHDVLSPLSTAMLALQLVQRACAQDPSVHRAT
                     ERGIAAVTRVRALVDGLLAFSRAGGQPEPGSSTEIAPVITDVVDALSGLASEQRIALC
                     ASAVPQGTIACTTGVLTSLCSNLVSNAIKYMADAPVRRIEVRTVDAGDRWRIEVADTG
                     PGIPLEDQERIFQPYVQLGGNRSGIGLGLATVDRLVRGHGGTLGVISAPRRGSLFWFE
                     LPKGPPRAM"
     gene            1927..2445
                     /locus_tag="VF516_02570"
     CDS             1927..2445
                     /locus_tag="VF516_02570"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HEX8106578.1"
                     /translation="MLNDAEKKLILNSWRLVVPIKDTAADLFYRRLFELKPAYQQLFK
                     GDMAAQKRKLIAMLAFIVKSLDWPDSAWRDNVAEEDDLFLVVLALGRRHADLYAVPDA
                     AYAAVGEALLWTLDYGLGKEFTPPVRAAWAKIYGLVATAMKMGRLSVQSQAQTEAMVR
                     RVVRTGAEDEPS"
     gene            2442..>4063
                     /locus_tag="VF516_02575"
     CDS             2442..>4063
                     /locus_tag="VF516_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106579.1"
                     /translation="MMLASGTLAELDVASIAATTSLGRSSLRLEVRAASGEPIGSLVL
                     KAGRIVSATAGAVHGRDALRVILSAGGDARFQLAREPLDFALSSALASVDELEKLARG
                     STGLARLSTHGGREATRSDLPSARRRTASLVAARVPMMEGQLAEFDLPRLLQTIGLGR
                     QLVEIELRDRHGVPRGAIQVKSSKIVSARAGEASGTAALSELIGARDCVAFAVFRIAG
                     DAGQLPVLASVSEAGVQLAPASSDPRDRTVVEGSLSEFDIPTILHTLGSSRQYSSLEV
                     HGEQATAGTIFLKSGIVQSAAAGSFTGLRAIQHLIASHPRDRFRLVQLADEIPEQVPL
                     GPVSHVLLSIDAPAPSPPVTRSPTAPVAPPRAATHAPEDRAAPPAGSLTAPSAAPELV
                     LMEGKLSDFDVRTVLEVLAATRQHARLQILDAGQPPLGEIALKAGQIISGQAGNLQGR
                     NALVFLLGASRQLWFRVVTGSPDLASREPLGSIHELLAGIEIGGVRRRETATTALRWA
                     IPLSFLVGGAIVFVVVRGGSGPSAGPPAAPAGR"
BASE COUNT          569 a         1452 c         1455 g          587 t
ORIGIN      
        1 aaaaacatga cacgggcggg cggcgcctgc aagtcgggcc tggatcgcca ggacgcggcg
       61 atccgagatt ccctggctcg tccgccttcg gcctgcaccg catctgcatc gccggtgctt
      121 gctttcgatc gcggctcaag caaggtccgg ccatggccga gtcaggctca tccgtatcgg
      181 tctcggcggc ccggtggctg gtcgcggtga tcgcgagtgt cgtgttgtgc ttcacggtcg
      241 cgagcgccgt cggagagtac ctggagagcg cgatcgggga gcgcgcgaac gacatcaccg
      301 ccgacgcgat gccgagcgtc cagctcctca ccaccgtcca gcgcgaggtc cagcagatcg
      361 atctcgacat ggaccgatac gcggcggcgc cggtcgagca gcgccccgag catcgcgccc
      421 ggatcatcgc cacgcgcaag gacgtcgccg cgtcgtttgc ctcgtacgcc gcgctcccga
      481 gccttgccga cgagcctctg ctccaggcgg acctccggac cagcctcgcc gacctcgacg
      541 acgccgtcgc ggactacttc tcggatggcc agccgcggtc gctcgcgcgc gtgcatgtcc
      601 agctcgatct cgccgaggaa gcgatcgcgc ggagcgtgtc gttcaatgcg gcacagggcc
      661 agcggctcgg gaccgagatc cagcacatcc gcagcgagac gcgaggcatc gtcgagctgc
      721 tcgatgtggc gagcgtcgtc ctggcgatcg gcgcctgcta cctcgcggtc cggcagctcc
      781 ggcgcgccgc gcgggaccag aaggccgagc gcgaggcccg cgagcgccgc gcggccgagc
      841 tcgcggtcca gaacgaggcg ctcgggcagt tcgccggccg cgtcgcccac gacgtcctga
      901 gcccgctgtc gaccgcgatg ctcgccttgc agctggtcca gcgtgcctgc gcgcaggatc
      961 cgtccgtgca tcgagcgacg gagcgcggga tcgccgcggt cacgcgcgtc cgcgcgctgg
     1021 tcgacggcct cctggcgttc tcccgtgcgg gtggccagcc cgaaccggga tcctcgacgg
     1081 agatcgcacc cgtgatcacc gatgtcgtcg acgccctgag cggtctggcg agcgagcagc
     1141 gcatcgcgct gtgcgcgagc gcggtcccgc aggggacgat cgcgtgcacc acgggtgtct
     1201 tgaccagctt gtgctcgaac ctggtcagca acgcgatcaa gtacatggcg gacgcgccgg
     1261 tgcgccggat cgaggttcgc accgtcgatg ccggcgatcg atggcgcatc gaggtggcgg
     1321 acacggggcc cggtatcccg ctcgaggatc aggagcggat ctttcagccc tacgtgcagc
     1381 tcggtggcaa ccggtccggc atcggcctgg gactggccac cgtcgaccgg ctggtccgcg
     1441 gacacggcgg gacgctcggc gtgatctcgg cgcctcggcg gggctcgctg ttctggttcg
     1501 agttacccaa ggggccaccg cgagcgatgt gaggggcacg ccccggtcgg cgctgacaga
     1561 gcgttggatc ggcgcggcgg tcacgcaggc tggactcgcc gcaggaagct cgggatctac
     1621 ggcacgtagc cgagtgtcta ccagcatcta ccgcggatgc tgcaaaacgc gacggtagcc
     1681 gcaacctccc ggtggggaaa cgatgcgtag gctatccctc cacggaggag gcccagccga
     1741 tggcagatcg caatctctcc gggggaacga aaaccctcgc cagaaccacg ggatcgcacg
     1801 atcaccggcc ggccgcggcc ctccccccgc gggcgcgggg aacacgcgtg ccgccgcaac
     1861 acgcggcgcg caaccgccgt cacgagcccc gcgggtttac cctcctttga ccaaggagca
     1921 aggcacgtgc tgaacgacgc ggagaagaag ctgatcctca acagctggcg cctggtcgtc
     1981 ccgatcaagg acaccgctgc cgatctgttc tatcggcggc tgttcgagct caaacctgct
     2041 tatcagcagc tgttcaaggg cgacatggcg gcccagaagc gcaagctcat cgcgatgctc
     2101 gcgttcatcg tcaagtcgct cgactggccg gactcggcgt ggcgcgacaa cgtggccgag
     2161 gaggacgacc tgttcctcgt cgtgctggcg ctcggccgcc gccacgcgga tctctacgcc
     2221 gtcccggacg cggcctacgc ggcggtcggc gaggcgctgc tgtggaccct ggactacggc
     2281 ctcggcaagg agttcacgcc gccggtgcgc gcggcctggg ccaagatcta cggcctggtc
     2341 gcgacggcga tgaagatggg gcggctcagc gtccagtcgc aggcccagac cgaggcgatg
     2401 gtccgcaggg tcgtgaggac cggagccgag gacgagccgt catgatgctc gcgtcgggca
     2461 ccctggcgga gctcgacgtg gcgagcatcg ccgcgacgac cagcctcggg cgctcgagct
     2521 tgcggctcga ggtccgcgcg gcgagcggcg agccgatcgg gagcctcgtc ctcaaggcgg
     2581 ggcggatcgt ctcggcgacc gcaggggccg tacacggccg cgacgcgctg cgcgtgatcc
     2641 tgagcgcggg gggcgatgcg cggttccagc tcgcgcgcga gccgctcgat ttcgcgctgt
     2701 ccagcgcgct ggcgtcggtc gatgagctcg agaagctggc gcgcggctcg accggcctgg
     2761 cccgcctgtc cacgcacggc ggccgcgagg ccacgcgctc cgatctgccg agcgctcgtc
     2821 gccgcaccgc atcgctggtc gcggcgcggg tcccgatgat ggaaggccag ctcgccgagt
     2881 tcgatctgcc gaggctgctc cagaccatcg ggctgggccg ccagctcgtg gagatcgagc
     2941 tccgcgatcg ccacggggtg ccgcggggag cgatccaggt caagagcagc aagatcgtgt
     3001 ccgcgcgcgc gggcgaggcc agcggcacgg cggcgctctc ggagctgatc ggcgcgcggg
     3061 actgcgtcgc gttcgccgtg ttccggatcg cgggcgatgc cggccagctc ccggtgctcg
     3121 ccagcgtatc cgaggccggt gttcagctcg caccggcgag ctcggacccg cgcgaccgca
     3181 ccgtggtgga ggggtcgctg tccgagttcg acatcccgac gatcctgcac acgctgggat
     3241 cgagccgcca gtactcctcg ctcgaggtcc acggcgagca ggccaccgcc gggacgatct
     3301 tcctcaagtc ggggatcgtg cagtcggcgg cggcgggctc gttcaccggg ctgcgcgcga
     3361 tccagcacct gatcgcgtcg cacccgcgcg atcgattccg tctcgtgcag ctggccgacg
     3421 agatccccga gcaggtcccg ctcgggccgg tcagccacgt gctgctcagc atcgacgcgc
     3481 ccgcgccgtc gccccccgtg acccggtcgc ccaccgctcc ggtcgcgccg ccccgcgctg
     3541 ccacgcacgc gcccgaggat cgcgccgcgc ccccggccgg ttccttgacg gctccctcgg
     3601 ccgcccccga gctggtgctg atggagggca agctctccga cttcgatgtc cggacggtcc
     3661 tcgaggtgct cgccgccacc cggcagcacg cgcgcctgca gatcctcgac gccggccaac
     3721 cgccgctcgg cgagatcgcg ctcaaggcgg ggcagatcat ctcgggccag gccggaaacc
     3781 tccagggccg aaacgcgctg gtgttcctgc tgggcgcctc gcggcagctg tggtttcgcg
     3841 tcgtgaccgg gtcgccggac ctcgcatcgc gggagccgct cggttcgatc cacgagctgc
     3901 tcgcggggat cgagatcggg ggcgtgcggc gccgcgagac cgcgacgacg gccctgcggt
     3961 gggcgattcc gctgtcgttc ctcgtcggcg gcgcgatcgt gttcgtcgtc gtccgcggcg
     4021 ggagcggccc ctccgccggg ccgcccgcgg cgccggccgg tcg
//
LOCUS       DASVOR010000123         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_381054,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000123 DASVOR010000000
VERSION     DASVOR010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381054"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VF516_02580"
     CDS             <1..273
                     /locus_tag="VF516_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106580.1"
                     /translation="AKVGGGASRVTARSTGNDQLTTMLTQITQSIQALAQNNNQSDPM
                     QMMMMMMMMGGMGGGGGGTVAAPPAAPAPPPAPVINITTTVRHHGW"
     gene            263..520
                     /locus_tag="VF516_02585"
     CDS             263..520
                     /locus_tag="VF516_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106581.1"
                     /translation="MAGKHAIEVIDLSELWYVCGGVDKQTQAAQDAIAQATSAVQNVA
                     DGLVPKNAGMTDMLMKVLSNRRSGSGPSSAPALPPTSTLSR"
     gene            580..771
                     /locus_tag="VF516_02590"
     CDS             580..771
                     /locus_tag="VF516_02590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106582.1"
                     /translation="MLRAVIGGRFTPRTTLDPVLLQGIQELSKAIASVGQNMAAAKQS
                     SSSQMVQMMQQMMQSRHGR"
     gene            1005..1316
                     /locus_tag="VF516_02595"
     CDS             1005..1316
                     /locus_tag="VF516_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106583.1"
                     /translation="MERIATMQDLNPTALTTVVGGTTTNDQITQQLSSLQTSLASLGN
                     NNNNTSSSSTMMMLAMCMAMKNQSTVIAGPSAAPPPMAAAPPPPVAAGPVVNINTRVR
                     H"
BASE COUNT          306 a          660 c          571 g          229 t
ORIGIN      
        1 gccaaggtcg gcggcggcgc ctcgcgcgtc accgcccggt cgaccggcaa cgatcagctg
       61 acgacgatgc tgacccagat cacccagtcg atccaggcgc tcgcccagaa caacaaccag
      121 agcgacccca tgcagatgat gatgatgatg atgatgatgg gcggcatggg cggcggcggt
      181 ggcggcacgg tcgccgcgcc tcccgcagcc ccggcgcctc cgccggcccc ggtgatcaac
      241 atcacgacca cggtgcgtca tcatggctgg taagcacgcc atcgaggtca tcgacctctc
      301 cgagctctgg tacgtctgcg gcggcgtcga caagcagacc caggcggcgc aggacgcgat
      361 cgcccaggcc accagcgcgg tccagaacgt cgccgacggc ctcgttccca agaacgccgg
      421 catgaccgac atgctgatga aggtgctgtc caaccggcgc tccggctccg gtcccagctc
      481 ggcgccggcg cttcccccca ccagcaccct ctcgaggtag ccatgagccg acgatccccc
      541 agccccgcgc cacgcgcgct ccagaccatc gacccgaggg tgctgcgcgc cgtgatcggc
      601 ggccgcttca cgccacgcac gaccctggat ccggtgctgc tgcaggggat ccaggagctg
      661 tcgaaggcga tcgccagcgt gggccagaac atggcggcag ccaagcagtc cagcagctcg
      721 cagatggtgc agatgatgca gcagatgatg cagtcgcggc acggccgctg acggcgacat
      781 cgaccgcgcg acgcgcgact tcggagctcg aggcttccgc ctcgggctct cgccgttttc
      841 ggcgcatcgc gcgccgcgcg tggcgccgcc cgccgccgcc gtcgtcggga agcaggccgt
      901 cactcgggat tgacaggatc gcgggcgccg cgatcgcgaa ccggctgatc tgattcggtg
      961 gcgcgcacgg cacggccgct gcacagaacg ccgatcaccc attcttggaa aggatcgcca
     1021 cgatgcagga cctcaacccg accgccctca cgaccgtcgt cggtggaacg acgaccaacg
     1081 accagatcac gcagcagctc tcgtcgctgc agacctcgct cgccagcctc ggcaacaaca
     1141 ataacaacac cagcagcagc agcacgatga tgatgctggc gatgtgcatg gcgatgaaga
     1201 accagtcgac cgtgatcgcc ggtccgtcgg cggccccgcc cccgatggcc gctgcgcccc
     1261 cgccgccggt cgccgccggc ccggtggtca acatcaacac ccgcgtccgc cactaggatc
     1321 cgctcgcctc acagacgccg ccgctgctcg aagagcacga tccacccggg tcgtgctctt
     1381 cggcgttttc ggcgccgaga tgctcgggct gctgggtggg gcgagatcgc ggatcaggag
     1441 cacgctcttc gtgcgcaggc agtctcgccg ctcgggcacc gtccgctcga ggcaacaacg
     1501 cgtctaccgc gatcggcgca cgcagccgcc ggggagcacg caacctcgca gaaccccgcg
     1561 cggcgagtgc cggcacgcac cctgcactac acgcggacat gcacggcatc gacaccatcg
     1621 agagcatcaa ccgcgagcaa ctcgccaccg cgagcgaccg gctgctgctg cgcggtcggg
     1681 tgtcgaggca gcgcccccgc ccctgggtcg cgccgcgcgg ctcgtggccc gagattccgc
     1741 acgggccgat cgcggagggt tcgcag
//
LOCUS       DASVOR010000124         2980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_176597,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000124 DASVOR010000000
VERSION     DASVOR010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176597"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VF516_02600"
     CDS             <1..1507
                     /locus_tag="VF516_02600"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8106584.1"
                     /translation="FRTALVSPRGVGGSSPARSGLSLHDYARDLAQVIEALAGQPAHA
                     IGHAFGNRVVRCLARDRPELVRSLVLLGAGGLVPPTCDAPAIWRQVVTGRRTDPARVA
                     AIKDGFFASSSDPTPWLDWCTEALASQAEAAQTPLGEWWDGGTAPMLVIQGREDKMAP
                     PDNALDLRARFGERVQLVEIPAAGHALLPEQPQLIAELTLHYLRTLRQLDVAPAAALP
                     ENGNPAQPRYALGYGAAMVRYLEARTGGSRARFFLDRVTSGMRILDCGCGPGTITLDL
                     AAAVAPTQVTGIDIEPSQIARARELAEHRNIANVRFEHADVYALPFADGSFEAAFLHT
                     VLMHLRDPVRALREVYRVLTPGGIIGVCDGDWGGDILTPANPILEQSRAITERMLNRR
                     GMSTRLGRHHRALLRDAGFVDVVASASVEAYGSPDANREFAAFNASMAEGYAAAAAAT
                     GSPAVAPGVTAQVIAAWQSWAEHPDGLFVRLRCEGLGRRPPATAVFHRLGE"
     gene            complement(1699..1989)
                     /locus_tag="VF516_02605"
     CDS             complement(1699..1989)
                     /locus_tag="VF516_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106585.1"
                     /translation="MGLDEVADPHAFGVVLHEGCPTLTAATFLPRTLRMYFWIVRLLT
                     LIPSLSSSPRMRVGNRLLTPGEPAVRPANGNAPVERRERLGGLLNFYYRPAA"
     gene            complement(2551..2799)
                     /locus_tag="VF516_02610"
     CDS             complement(2551..2799)
                     /locus_tag="VF516_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106586.1"
                     /translation="MIALAEHAPGGDPRVPEDVDLRVGGGSTETRPGDILTALSQRLW
                     LAQQSYPDLVGPIGEVGLRSRRTTPHRRRRPVPRGRPA"
     gene            complement(2796..>2980)
                     /locus_tag="VF516_02615"
     CDS             complement(2796..>2980)
                     /locus_tag="VF516_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX8106587.1"
                     /translation="RAAHDRNKVSFEWQRGDVLLIDNMLVCHGRDPFDGSRRVLVCMA
                     EPYSEVRRMCATLGPR"
BASE COUNT          441 a         1059 c         1028 g          452 t
ORIGIN      
        1 ctttcgtacc gcgctggtca gcccgcgcgg cgtcggcggc agctcgccgg cgcgctccgg
       61 gctgagcctc cacgactacg cgcgcgatct ggcgcaggtc atcgaggcgc tcgccggcca
      121 gccggcgcac gccatcggtc acgcattcgg caaccgcgtg gttcgctgtc tggcgcgcga
      181 tcgcccggag ctcgtgcgca gcctggtcct gctcggcgcc gggggcctcg tgccaccgac
      241 gtgcgacgcg ccggcgatct ggcggcaggt ggtgaccggc cggcggaccg atccggcacg
      301 cgtggccgcg atcaaggacg ggttcttcgc gtcgtcgtcg gatcccaccc cgtggctcga
      361 ctggtgcacc gaggcgctcg cgtcgcaggc agaggctgcg cagacgccac tcggcgagtg
      421 gtgggacggc ggcaccgcgc cgatgctggt gatccagggt cgcgaggaca agatggcccc
      481 gccggacaac gctctggatc tgcgcgcccg gttcggcgag cgcgtgcagc tcgtcgagat
      541 cccggccgcc gggcatgcgc tgctgccgga gcagccacag ctgatcgccg agctgaccct
      601 ccactacctg cgcaccctgc gccagctgga tgtcgccccc gccgccgcgc tccccgagaa
      661 tggcaacccg gcccagccgc gctacgccct cggctatggc gctgccatgg tgcgttatct
      721 cgaagcgcgc accggcggaa gtcgcgcccg gttcttcctc gaccgggtca ccagcggcat
      781 gcgcatcctc gactgcggct gcggtccagg aaccatcacc ttggatctcg ccgcggccgt
      841 ggcgccgacc caggtcaccg gcatcgacat cgagccgagt cagatcgcgc gcgccagaga
      901 gctcgcggag caccgcaaca tcgcaaacgt ccgcttcgag cacgcggacg tctatgcatt
      961 gccgtttgcg gacggctcgt tcgaagcagc cttcctgcac accgtgctca tgcatctccg
     1021 cgacccggta cgggcgctgc gcgaggtcta tcgcgtcctc acgccagggg gcatcatcgg
     1081 cgtgtgcgac ggcgactggg gcggcgacat cctcacgccc gccaacccga tcctggagca
     1141 gtcgcgtgcg atcaccgagc ggatgctgaa ccgccgcggg atgagcacgc gcctcggacg
     1201 gcaccatcgc gcgctgctac gcgacgccgg attcgtcgat gtcgtcgctt cggcgtcggt
     1261 cgaggcctac ggcagtccgg acgccaaccg cgagttcgcc gccttcaacg ccagcatggc
     1321 cgagggctat gccgcggcag cagctgcgac cgggagcccg gctgtcgcgc cgggcgtcac
     1381 cgcgcaggtc attgctgcat ggcagagctg ggccgaacac cccgatggat tgttcgtgcg
     1441 gctgcgctgc gaaggactgg gccgtcggcc accggctact gccgtgttcc accggctcgg
     1501 cgaatgacgg tcacgacggc cggagcaggc gcgccgtcgt gtgccgatta accggtcagt
     1561 ctggccgcgg agcacggcct atggtttcgg ggagctgagc ttccgaattt catgccgcag
     1621 ccgctcgatg cgtttgagca gagcgctcag gtcgcgccgg gacgggacgc cgtcgtgtgc
     1681 cagaagtcga tcgcgagctc atgcggcggg ccggtagtag aagttgagca gtccgcccag
     1741 ccgctcgcgg cgctcgaccg gcgcgttccc gttggctggt cgcaccgccg gttcgcctgg
     1801 cgtgagcagc cggttgccca cgcgcatccg cggcgagctg ctcaagctcg ggatcaaggt
     1861 cagcaagcgg acgatccaga agtacatgcg cagtgttcgc ggcagaaatg ttgcggcggt
     1921 cagcgttggg caaccttcgt gaagaaccac gccgaacgca tgtgggtctg cgacttcatc
     1981 cagacccacg acctctcgtc gtgtgccgaa gcccgccggc aggaatgcga tgcgtatcca
     2041 agcgttgccg tgctcgtcgc gggccccagg tcgatcaccg gcagcgctac catggtgccg
     2101 cgatgggcac cgaagctgtg cggttcttgg tcctctggat ggcgggctgg atccactccc
     2161 gtcagctcga ggtcatcgac ttcctgcgcg acgagaaccg cgtgctccgc gagcagctcg
     2221 gcggacggcg cctacgtctt caccgacgac cagcggcgac gcctggccgt caagggccgg
     2281 atcgtcggcc gtcgcaggct cggcgagttc gcggggctgg tcacgcccga cacgatcctg
     2341 cgctggtacc gcgagctgat cgcccggcaa gtacgacggc tcggcgcgcc gctggaccgg
     2401 gcgtcctact gtcgccgccg acgtggagca gctcgtcgtc aggatggcga cggagaatcc
     2461 gagttggggg tacacgcggc tggtcggcgc gctggacaac ctcggccacc aagtcggacg
     2521 caacacggtc gaatggaacg ccccacttcg tcatgccgga cggccgcgcg gcacgggccg
     2581 cctccgacgg tgtggtgtcg ttcgccgcga acgcaagccg acctcgccga tcggccccac
     2641 caggtccgga tagctctgct gcgcgagcca caaccgctgc gacagggcgg tcaggatgtc
     2701 tccgggccgg gtctccgtag aacctccgcc gacgcggagg tcgacgtcct cgggcacccg
     2761 cgggtcgccg ccaggggcat gctcggcgag cgcgatcacc gcggacccag cgtcgcgcac
     2821 atgcgccgga cctccgagta aggctccgcc atgcagacca gcacccgccg gctgccgtcg
     2881 aacggatctc ggccgtgaca gaccagcatg ttgtcgatca gcaacacgtc gcctcgctgc
     2941 cactcgaacg agaccttgtt gcggtcgtgc gccgcacgga
//
LOCUS       DASVOR010000125         7817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_59619,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000125 DASVOR010000000
VERSION     DASVOR010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7817
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59619"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF516_02620"
     CDS             complement(<1..206)
                     /locus_tag="VF516_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106588.1"
                     /translation="MAVDRAPLLDRRFLDALDALAAQGDRPPLDLDAPIRPGSRLTAR
                     LAIDLFDAQAASRHLDYAARALRAR"
     gene            348..1202
                     /gene="dat"
                     /locus_tag="VF516_02625"
     CDS             348..1202
                     /gene="dat"
                     /locus_tag="VF516_02625"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047810 - D-alanine:2-oxoglutarate
                     aminotransferase activity [Evidence IEA];
                     GO_process: GO:0019480 - L-alanine oxidation to pyruvate
                     via D-alanine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HEX8106589.1"
                     /translation="MTTVYLNGEYVPHERAVISVDDRGFVFGDGIYEGIRAIEGQIFE
                     WQAHADRMIDGLTGIRIEFGADRVAALRGVCERLLRDNQLTTGDAFLYLQVTRGSAPR
                     AHAFPPAGTPPSVYVSASRLARRVDQQQSGVKAVTYPDLRWARCDWKTINLLGSVLAR
                     QAAVDAGAFEAVMLRDGVVTEGASSTIFGVVDGVLRTHPLGHRILPSVTRKVVMALIA
                     ELRLPVEERAISEAELRRVDELFLCGTANNVTPIISLDGRPVGAGTPGPLTAQLREAL
                     DARLYGRR"
     gene            1628..3409
                     /locus_tag="VF516_02630"
     CDS             1628..3409
                     /locus_tag="VF516_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106590.1"
                     /translation="MCGVAAAVLGIAGAAQAAPCGLLDDGASHPPPTTGSFGYDTFIP
                     PAAGQSYQDPVTHCNVRRLTQSQSQFNHIPMYHEYAPIRAVNADNTRVLGVTDDGFWF
                     VLNVDGTVNVPPYGAVTLTRPRWNPQVANSLYDMTGSTLYRVDISNGAFVRTALHTFT
                     GYSQVIFPRHNDIAEDGDYLALEGLTSSGTADAFIYRVSTSWTSPTHAMGTYDPHGDE
                     PEVTPNHAVLIGGKVYDLNMNFVRDLGVSGGWHHAIGRDVDGQDIFVLPASPDPNTVC
                     SGFGGGNGDGVEKIRIATGAKTCLFGFPGWWDGSHVGVSSNGWVVFSATDYTDGTANI
                     DLPANWASQWSPAKNEIILIRADGSQVYHLAQHRSRPVGLPTDPGYWAMPRANINADG
                     SLVIFDSNMGLDHRLGYPDDYADVYLIATGASGGTTQGTQPVTWTGPVNASAFGSDGS
                     GLHKTSGCDGCDDAGAVSSQQIASGDGNIEVTASATPGTLYIGLNNVSNDTTAWEIPF
                     AFAIWSSGIAEIREYGVYQTETTFVAGDRLRVAYASGQVRYYKNGQLLYTSARTPLYP
                     AFADTSLWTYNPAAGQVATVDRVSITR"
     gene            3549..3758
                     /locus_tag="VF516_02635"
     CDS             3549..3758
                     /locus_tag="VF516_02635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106591.1"
                     /translation="MDFLWGLWNGITAWPLLILHAFGALGHYPIYDVARDGGWYQFGF
                     LLGAGSPFLGMLGNKKARSRPSDGS"
     gene            complement(3735..4673)
                     /locus_tag="VF516_02640"
     CDS             complement(3735..4673)
                     /locus_tag="VF516_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8106592.1"
                     /translation="MNRANRPVLERLDLNLLVTFEAVYRERNLTRAAKRLFVTQSAVS
                     HALARLREQLGDPLFVRRASGVEPTPAAVRLAPQVGEALRLLRRALETDEFDPARDLG
                     RVRIAIHEELEPAVLPAFERRLRAAVPGVELDCVRVDRTSLERDLGSSRLDLAVDAAQ
                     VAGADLRHASLAHDQFCVVSRRGTRIDVRRYLAARHVMVSSRRTGPALEDVLLSQLGV
                     QRTVVLRCQRYEAACRIVADSDLLLTAPRLRARAIAAHMRLVLLPVPFALPPFDMRLY
                     WHRQLDTDLRSQWLRAQLTAVAAELGIGRSRAIGRA"
     gene            4735..5976
                     /locus_tag="VF516_02645"
     CDS             4735..5976
                     /locus_tag="VF516_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8106593.1"
                     /translation="MDFTPSAKSTDHIQRVRRFIQDHIAPVEHRYWDEVAAMNPTGDW
                     RTWKIHPLVEELKAKARQAGLWNMFLPGAPGGLSVLDYAPVAEEMGRSFLAPEVFNCA
                     APDTGNMEVLWRYGSDEQKQRWLAPLLAGEIRSVFCMTEPDVASSDATNMRATAIVEG
                     DHVVLNGKKWWSSGIGDPRCKIAIFMAHTPSEAADRHHQHSQVLVPIDTPGVKIQRML
                     PVFGSYDAPHGHGEVHFDNVRVPRANIIAGPGMGFEIAQGRLGPGRIHHCMRCIGAAE
                     QTLDLMIHRGMSRTAFGKPLIDLGGNRERVAEARLAIDQARLLTLYAAWKLDQVGALG
                     AMTEISAIKVVAPNMLQKIVDDAIQIHGGAGVSEDTPLTSFFAQARTLRLADGPDEVH
                     KGVIARIELTKRGYARSRGKH"
     gene            6540..7520
                     /locus_tag="VF516_02650"
     CDS             6540..7520
                     /locus_tag="VF516_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106594.1"
                     /translation="MLNEHADRKGSVFERPTPRWDPRKEVNRWLGEITLMHSFATSEG
                     RSIPPETAKAMAELIRKNPTDPATEPAEVLPKILEIHGALAALVAPATPRSIRATEDE
                     EGGVVSWWDNKLIRYLILLAGLSLIAFFVSAIWTTFPARREVGWLAAAILGASFYNLF
                     TAYKYILDRTFDPQYKTIYAVRYVLGVVSGFVLANFGDQLGLSKVNGLSIGGPLAALI
                     GGYSSDAVNQILLRVSEILSAVVKGSGAAAVERRATSAAPLATSPVPSTDSTLAVAAS
                     TKRRAALAEVAPSAPSAASTGSTLAVVEQAAASTAGATGTTVVGPPSAAA"
BASE COUNT         1232 a         2731 c         2720 g         1134 t
ORIGIN      
        1 cgggcgcgca gcgcgcgggc cgcgtagtcg aggtggcggc tcgccgcctg ggcgtcgaac
       61 aggtcgatcg ccagccgcgc ggtcagccgc gagcccggcc ggatcggcgc gtcgagatcc
      121 agcggcggcc ggtcgccctg cgcggcgagc gcgtcgagtg catcgaggaa cctgcggtcg
      181 agaagcggag cgcggtccac ggccatgcgg cggttatact cggttcgcgc ggtgcgctgt
      241 cgcggcccgc gccgccggcg ccctgccccg gcggttcccg ccacgagctc gcaagaaccg
      301 cgctccggat gctggacatt cccgccggcc gtgattccct gacagtcatg accaccgtgt
      361 acctgaatgg cgagtacgtc ccccacgagc gcgccgtgat ctcggtcgac gaccgcggct
      421 tcgtgttcgg cgacggcatc tacgagggca tccgcgcgat cgagggccag atcttcgagt
      481 ggcaggccca cgccgaccgc atgatcgacg gcctgaccgg catccgcatc gagttcggcg
      541 ccgaccgcgt cgccgccctg cgcggcgtgt gcgagcgcct gctgcgcgac aaccagctca
      601 ccaccggcga cgccttcctg tacctccagg tcacacgcgg cagcgccccc cgcgcgcacg
      661 cgttcccgcc ggccggcacg ccgcccagcg tctacgtcag cgcgagccgg ctggcgcgcc
      721 gcgtcgatca gcagcagtcc ggcgtcaagg ccgtcaccta tcccgatctg cgctgggcgc
      781 ggtgcgactg gaagacgatc aacctgctcg gctcggtgct cgcccgccag gccgcggtcg
      841 acgccggcgc gttcgaggcc gtcatgctgc gcgacggcgt cgtcaccgag ggcgcctcgt
      901 cgacgatctt cggcgtggtc gacggcgtgc tgcgcaccca tccgctcggc caccgcatcc
      961 tgcccagcgt cacccgcaag gtcgtcatgg cgctgatcgc cgagctccgc ctgccggtcg
     1021 aggagcgcgc gatcagcgag gccgagctgc gccgcgtcga cgagctgttc ctgtgcggca
     1081 ccgccaacaa cgtcacgccg atcatcagcc tcgacggccg gcccgtcggc gccggcaccc
     1141 ccggcccgct caccgcccag ctccgcgaag ccctcgacgc ccggctctac ggccgccgct
     1201 gatcggcatc ctgcgtggta tcgccccgcc agcctccgca tcccggcccg atcgttccgg
     1261 cggcctcggg ggtcaggatg gctggtgcca agctcgcccg gggacggtag cgagcggcgg
     1321 aatgcttggg gcgggcgata tggtccaccg cggtggcgcc tgctcacgca gcgtggcagc
     1381 gcggggctcg tcatgcgctg cgacaagccg ttccagccgc tgcgaggagc caggtgccgg
     1441 cccgcggcga cccgtgccgg gctgcctcat cgggcgctcg tgcaatgtgg ccatatcgag
     1501 tgtcgcagtc ggcgggcgcg gcctcggcag aggcatgatc gggtgtccgc cgcgcaactg
     1561 cgcgacgcgc tccgggcatg tccagtgcac tgaccgtctg cggaggtaga tcatgcgcga
     1621 tgttctgatg tgtggagtcg ccgcggcggt cctcgggatc gccggcgcgg cgcaggcggc
     1681 cccgtgcgga ctgctcgatg acggcgcgag tcatccgccg ccgacgaccg gcagcttcgg
     1741 ctacgacacg ttcattcccc cggccgccgg gcagagctac caggacccgg tgactcactg
     1801 caatgtgcgg cggctgaccc agagtcagag tcaattcaat cacatcccga tgtatcacga
     1861 gtatgcgccg atccgcgcag tcaacgccga caacaccagg gtgctgggcg tgaccgacga
     1921 cgggttctgg ttcgtgctca acgtggatgg cacggtgaac gtcccgccct acggtgcggt
     1981 gacgctcacc cgcccgcggt ggaatcccca ggttgcgaat tccctgtatg acatgaccgg
     2041 cagcacgctg taccgcgtgg acatcagcaa cggggcgttc gtgcgcacgg cgctgcacac
     2101 gttcaccggg tacagccagg tgatcttccc gcgccacaac gacatcgccg aggacggtga
     2161 ctacctcgcg ctggagggcc tgacctcgag cggcaccgcc gacgcgttca tctaccgggt
     2221 cagcaccagc tggaccagcc cgactcacgc gatgggcacc tacgacccgc acggcgacga
     2281 gcccgaggtg acgccgaacc acgccgtgct gatcggcggc aaggtctacg acctcaacat
     2341 gaacttcgtg cgcgacctcg gggtcagcgg cggctggcac cacgcgatcg gccgggacgt
     2401 ggacggtcag gacatcttcg tgctgccggc ctcgccggat ccgaacaccg tgtgcagcgg
     2461 gttcggcggg ggcaacggcg acggcgtcga gaagatccgg atcgccaccg gggccaagac
     2521 ctgcctgttc ggctttcccg gctggtggga cggcagccac gtcggcgtca gcagcaacgg
     2581 ttgggtcgtg ttcagcgcga ccgactacac cgacgggacg gcgaacatcg acctgcccgc
     2641 gaactgggcg agccagtggt cgccggccaa gaacgagatc atcctgatcc gcgccgacgg
     2701 ctcgcaggtg taccacctcg cccagcaccg ctcgcggccg gtcgggttgc cgacggatcc
     2761 cggctactgg gcgatgccac gcgccaacat caacgccgac ggctcgctcg tgatcttcga
     2821 cagcaacatg gggctcgatc accggctcgg ctatcccgac gactacgccg acgtctacct
     2881 gatcgcgacc ggggcgtccg gcggcacgac ccagggcacg cagccggtga cgtggaccgg
     2941 cccggtcaac gcatcggcgt tcggctccga cggcagcggg ctgcacaaga cgtcgggctg
     3001 cgacggctgt gacgatgccg gcgcggtgtc gagccagcag atcgcgtcgg gcgacggcaa
     3061 catcgaggtc accgcgtccg ccacgccggg cacgctgtac atcgggctca acaacgtcag
     3121 taacgacacg accgcgtggg agatcccgtt cgcgttcgcg atctggtcga gcgggatcgc
     3181 cgagatccgc gagtacggcg tctaccagac cgagacgacg ttcgtggcgg gcgaccggct
     3241 gcgcgtggcc tacgcgtccg gccaggtccg gtattacaag aacggtcagc tgctctacac
     3301 cagcgcgcgg acaccgctct accccgcatt cgcggacacc tcgctgtgga cctacaaccc
     3361 ggctgccggc caggtcgcca ccgtcgatcg ggtctcgatc acacggtagc gacgatccgg
     3421 aggcgacgcg caacggctgc ggcccgccgc cggtccgctg caagcgttgc cggcggtgcc
     3481 ccaccgccct cgcgattgcg ccggcacgcc gcatgcgagc gcgctaccct ggaagccagg
     3541 agaaccatat ggacttcctc tggggcctct ggaacggcat cacggcatgg ccgctgctca
     3601 tcctccacgc gttcggcgcg ctcggccact acccgatcta cgacgtcgcc cgcgacggcg
     3661 gctggtacca gttcgggttc ctgctcggcg ctggatcgcc gttcctgggc atgctcggca
     3721 acaagaaggc gagatcacgc ccgtccgatg gctcgtgacc gaccgatgcc gagctcggcg
     3781 gcgacggccg tgagctgggc gcgcagccac tgactgcgca ggtcggtgtc gagctggcgg
     3841 tgccagtaca ggcgcatgtc gaacggcggc agcgcgaacg gcaccgggag cagcacgagg
     3901 cgcatgtggg cggcgatcgc gcgggcgcgc agccgcggcg cggtgagcaa gaggtcggag
     3961 tcggcgacga tccggcacgc ggcctcgtag cgctggcagc gcagcacgac ggtgcgctgg
     4021 accccgagct ggctgagcag gacgtcctcg agggcggggc cggtgcggcg cgacgacacc
     4081 atgacgtggc gggcggcgag gtagcggcgg acgtcgatcc gggtgccgcg ccggctgacc
     4141 acgcagaact ggtcgtgggc cagcgacgcg tggcggaggt cggcgccggc gacctgggcg
     4201 gcgtcgacgg cgaggtcgag ccggctcgac ccgaggtcgc gctcgaggct ggtgcgatcg
     4261 acccggacgc agtcgagctc gacgccgggg accgcggcgc gcagccggcg ctcgaacgcg
     4321 ggcagcaccg cgggctcgag ctcctcgtgg atcgcgatcc ggacccggcc gaggtcgcgc
     4381 gcggggtcga actcgtcggt ctccagcgcg cggcggagca ggcgcagcgc ctcgccgacc
     4441 tggggcgcga gccggaccgc ggccggggtc ggctcgacgc cgctggcccg gcgcacgaac
     4501 agcggatcgc cgagctgctc gcgcagccgc gccagcgcgt ggctgacggc ggactgcgtc
     4561 acgaacaggc gcttcgccgc ccgcgtcagg ttgcgctccc ggtacaccgc ctcgaacgtg
     4621 accagcaggt tgaggtcgag gcgctcgagc accgggcgat tcgcgcggtt catgatgcga
     4681 tcttccgaac attcagttcc ttcatgcaag ccgcggtcgt accttgtagc cgccatggac
     4741 ttcaccccga gcgccaagag caccgaccac atccagcgcg tgcgccgctt catccaggac
     4801 catatcgccc cggtcgagca ccgctactgg gacgaggtcg ccgcgatgaa cccgaccggc
     4861 gactggcgaa cctggaagat ccacccgctg gtcgaggagc tcaaggccaa ggcccgccag
     4921 gccgggctgt ggaacatgtt cctgcccggg gcgccgggcg ggctgtcggt gctggactac
     4981 gcgccggtcg ccgaggagat gggccgcagc ttcctcgccc ccgaggtgtt caactgcgcg
     5041 gcgcccgaca ccggcaacat ggaggtgctg tggcggtacg gctccgacga gcagaagcag
     5101 cgctggctcg cgccgctgct cgccggcgag atccgctcgg tgttctgcat gaccgagccc
     5161 gacgtcgcgt cgtcggacgc gaccaacatg cgggcgaccg cgatcgtcga gggcgaccac
     5221 gtcgtgctca acggcaagaa gtggtggtcg agcggcatcg gcgacccgcg ctgcaagatc
     5281 gcgatcttca tggcccacac cccgtcggag gccgccgacc gccaccacca gcactcgcag
     5341 gtgctggtgc cgatcgacac cccgggcgtg aagatccagc gcatgctgcc ggtgttcggc
     5401 tcgtacgacg cgccgcacgg ccacggcgag gtccacttcg acaacgtccg ggtgccgcgc
     5461 gccaacatca tcgccgggcc gggcatgggc ttcgagatcg cgcagggccg gctcggaccg
     5521 ggccggatcc accactgcat gcgctgcatc ggcgccgccg agcagacgct cgacctgatg
     5581 atccaccgcg ggatgtcgcg caccgcgttc ggcaagccgc tgatcgacct cggcggcaac
     5641 cgcgagcggg tcgccgaggc ccggctcgcg atcgaccagg cgcggctgct cacgctgtac
     5701 gccgcgtgga agctcgatca ggtcggcgcg ctcggcgcga tgaccgagat ctcggcgatc
     5761 aaggtggtcg cgcccaacat gctgcagaag atcgtcgacg acgcgatcca gatccacggc
     5821 ggcgccggcg tgtccgagga caccccgctg accagcttct tcgcccaggc ccgcacgctg
     5881 cggctcgccg atggccccga cgaggtccac aagggcgtga tcgcgcggat cgagctcacc
     5941 aagcgcggct acgcgcgatc gcgcggcaag cactagcaga tctgatttac tgtcggaacc
     6001 ccagcgcggc cactgcggac cagcgaatcg ccgcagcaat tcgccccggc gtccgacagg
     6061 attgcacccg cgttttcgga gcccggcgaa gatcggcgct ccgagaacgc gaatcgcgtg
     6121 cggcggtgtg acgcgtcatc cgagcaaatt gcgggcttgt gcacgggaga gaaaattaca
     6181 gtctcgccga cgatcacgaa ccacaaggcg gttgcgtcgt ttgcgatcac cgcgcgcggg
     6241 tcgccgcggc cacgcaggta gtgggccggg tcgtcgtgcg gacgagcgaa gtcgtcgttc
     6301 cgacgggcgg gctacgcggt ggctcgatct gagcaccgac accaaccgcc ggtcgatcgg
     6361 agatgagagc ggacaccggg cgttgcgcgg catgaaagct gcttcctccg atctccgggg
     6421 ggcgcactcc attcatcaag tgctcacgga ctccgtgcgc gtacgcaggc ccggacgtgt
     6481 gcgcaggccc gacgtgtgct cgcgtcattt cgtggcgccc acgaaccagg aggttccgga
     6541 tgctgaacga acatgcggac aggaaaggca gcgttttcga gcggcccacg ccgcgctggg
     6601 atccgcgaaa ggaggtcaat cggtggctcg gcgagatcac gctcatgcat tcgttcgcga
     6661 cctccgaagg aaggtcgatt ccacccgaga cggccaaggc catggccgag ctcatccgga
     6721 agaatccgac tgacccagca acggagcccg cagaggtcct gccgaagatc ctggaaatcc
     6781 atggggcgct cgcggcgctg gtcgcgcccg caactccgag gtcgattcgc gcaacggaag
     6841 atgaagaagg cggcgtcgtg tcgtggtggg acaacaaact catccgctac ctcatcctgc
     6901 tcgccggact ctcgctgatt gcgttcttcg tttcggccat ctggacaaca ttcccggcca
     6961 ggcgagaggt gggctggctc gccgcggcga tcctcggggc ctcattctac aatctcttca
     7021 ctgcatacaa gtacatcttg gaccgcacgt tcgatccgca atacaagact atctatgcgg
     7081 tgcgttacgt cctcggcgtg gtctctggtt tcgtgctcgc gaacttcggt gaccagctcg
     7141 gcctctcgaa ggtgaatggc ctgagcatag gcggcccgct cgcggcgctc atcggaggct
     7201 actcctctga cgcggtcaac cagatcctgc ttcgcgtgag cgagatcttg agcgccgtgg
     7261 tgaagggtag cggcgcggcc gctgtagaac ggcgcgcgac gtcggctgcg ccacttgcga
     7321 cgagtccggt cccgagcacg gattcgacgc tggcggtggc ggcctcgacg aaacggcgcg
     7381 cggcgttggc cgaggtagcg ccaagtgctc cgtcggcagc gagcacgggt tcaacgctgg
     7441 cggtggtgga gcaagccgcg gcgagtacag cgggggccac gggtacgacg gtggtggggc
     7501 cgccttcagc ggcggcctaa cccgggtgct ggccccagcg gtgcattttt cgcgctcttc
     7561 gcgcattcgg gtgacgcctc gtcgagcaac ctccgagcta cgtcggcgcc cgctggtagc
     7621 gctgccgagc agcggctcgc tcagggtttg acgatgcgga cgccggcgaa ctggatgacg
     7681 ccgccgctgt tgaccgcgct cgccgagctc gcgaccagcc gcaacgtggt cgacgtctga
     7741 agcgcgaacg agttcatcac cgtgtagatc gtcgtgtacg cactgccgtc cggggagatc
     7801 tcgaagtacc agacgcc
//
LOCUS       DASVOR010000126         7779 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_351952,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000126 DASVOR010000000
VERSION     DASVOR010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7779)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7779)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7779
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351952"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /locus_tag="VF516_02655"
     CDS             <1..1000
                     /locus_tag="VF516_02655"
                     /inference="COORDINATES: protein motif:HMM:NF024053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8106595.1"
                     /translation="GPGGPKARAGGDHEGSETRGAVEGSRRGSALGVEGGSEGGRFGG
                     EGKPGDDGVTMGGAIAGGVIAVPEAIKGAVDVLLIADAGDVSGAGSQLFGQLGKEIGK
                     MSAAALRQVVAREARAAVEHELRATIERMARTPRWQALTAEERERAVRIAYYELQRRF
                     FRGFGDTAKAADRSATKALRGATGARKIAAQESLDAARVGAEAAEIEPVAGRLPRNHR
                     YAGKEYPRELLPPKYREKGLRFKPSGHPDFEPYALTLPNGRKTVKIELTGSRRADEAL
                     ANKAAGFKGEPPSTHAWHHVEDEGTMMLVPKDLHREVPHTGGRATFKHRTGVDYGD"
     gene            990..1538
                     /locus_tag="VF516_02660"
     CDS             990..1538
                     /locus_tag="VF516_02660"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX8106596.1"
                     /translation="MGTSWAPFYETGPRVTEDDVRRFEREFGHELPADYRAFLVEVNG
                     GHVRRSHSVFTLRRGAHKDESVLRGLFSLNAPDDQDDLATAQKHYNPDVKLPEGFLEI
                     GYDGFGCPVVLPLVGQHRGEVWYLDIEDDGEDIEDDSEDRPSVRVEWFDRRDVWKLAD
                     SFAEFMAGLRPLEDDASASASP"
     gene            1730..4942
                     /locus_tag="VF516_02665"
     CDS             1730..4942
                     /locus_tag="VF516_02665"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106597.1"
                     /translation="MHPIRIALPLVLLGLLAAAAAADPVPEASYQQLHWRMVGPFRGG
                     RTRAVAGVPSQPSVFYIAQVNGGVWKTDDYGQTWRPIFDEQPTQSIGSIAVAPSDPKI
                     IYVGSGEGLHRPDLSVGNGVYKSTDAGKTWTHLGLDDAQQIPQIAVDPRNPDRVYAAV
                     LGHPYGPSEQRGVFRSLDGGKTWARVLYKDANTGASDLEIDPRHPDVLYAALWESRLG
                     PAEDGNEFRGTGGGLYKSTDGGTTWRKLEGGGLPANGVQFDLAIAPSQPSRLYCELST
                     TEPGAYGTSRGAGLYRSDDAGATWTRITHDDRPAMKIGGGDLMVPVVDPRNPDVVYVA
                     SIVAMKSTDGGKTWTWLRGAPGGDDYQNLWINPNDPKVFALVSDQGANITVNGGKTWS
                     SWYNQPTAQLYHVGVTADFPYRICSGQQESGSVCISSRGNDGSIGYREWRPVGIIEYG
                     YAAPDPRNPDIVYGAGRNEVSRYQWSTGRVQNVTPIPIRGSYRTERTQPIVFSPLRPS
                     VLYYASSVLFESADGGQSWRTISPDLGHPHPGVPPSVGALAASNKAAEDARGAIYALA
                     PSFKAPGTIWAGTDDGKLWVTRDGGGHWTDITPPELTPWSKVTQLEASHFDDVTAYAS
                     VSRFRIDDLAPYIYRTHDGGKTWTLITHGLPPGPVDAVREDPVRKGLLYASTENGVWV
                     SFDDGAAWQSLQRNLPHTSVRDLVVHDSDLIVATHGRGFWIMDDVSALRQIAADPRTA
                     MSDTLFKPAPAYRVPHSLYTDTPVPPDEPLAENPPTGAVIDYYLAQPAQGPVMLEISD
                     ARGQLVRRYASTDPPDLTAGEIARQLIPSYWVRPHRSLGTTAGAHRWVWDLRGARPLA
                     TSYSYPISAVPLDTVRTPLGPWVLPGGYTVKLTVHGKTLSAPLEVRLDPRIKLAPAAL
                     AQRHQLALRLSDLVSRSSQLVLHAQSTVDQLTRLAGKPPAAQKDPIEATLARVTAVLS
                     GPKGPPPAPGRERPPSLTGVNGKLVTLYRMVDVDAAPTAVQGTETAKAERELTQLAAA
                     WDGVRTGELAALNAALTAAGQPAIRPELAPQTQQDEGDEE"
     gene            5037..7592
                     /locus_tag="VF516_02670"
     CDS             5037..7592
                     /locus_tag="VF516_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX8106598.1"
                     /translation="MSARVVCFAVAVAIEIGAAQHAHALDGHRRVTQYAQTHFAARDG
                     MPHGVTTAIVQTDDGYLWTASHEGLSRFDGAAFTNFDHRGTEGVPSNTFTALAVDAAG
                     MLWAGTRDHGLLHLVGGEFQAVVWEPGPQNQQIRALAFDGNGDLWVGTRDRGVVRLHA
                     GVLTAALTTQDGLPSDDIRALYRTRDGAIWIGTFHGLVQWKAGQLVRGPAALDGTTVH
                     SIAQGPRGDLWCATDKGLAHLHDDVVDILDEKRLSATDIQSVLFDRDGNLWFGTGAGL
                     ARMTFGAAGVQGGIQIERLPSPDVLILALFEDSDGNLWIGSERGLDRLRDGDALPFGA
                     SEGLTDDVVLGFREDPTGARWLTTSSGLYRVEPGQTTATRLVADHVMYAILPDSHGDV
                     WFGGRNGDVGRWHDGQFTWLGKARWERVRAIRETVDGMWFGTDHGLFRMRGERLSDAE
                     VVVEGIAVRGIVPDSDGSLWLATENSGLLRWRAGARAEIPSGGPPRDIPTATITFDSD
                     GTMWVGTEGGGLWRLRDGRWVAFTTKDGMFDDVVWRILDDGLGNLWMSSNRGIWRVSR
                     DQLETRAVGLRPTIDSAVYGEADGMRDRECNGANDSAGWRTHDGWLWFPTGKGLVAID
                     PAHLRPGRPSRAVVETVRIDGQPQRVTDRLVLPADTGRLELGYTAPLLRGPQRLRFRY
                     QLESFDRGWNDAGPQRTAQYTNLPPGDYRFRVEAGLDGEWGKPATLRVTLPPRFYQNA
                     WFYVLAILSVALAIVAVPLLRVRQLRARARELDRRVQEAISELKVLSGLLPICAWCKK
                     VRDDHGYWNRIEAYLSARTDAQFTHGICPDCTKKVMAEDSALASQSVTSVKRP"
BASE COUNT         1190 a         2785 c         2797 g         1007 t
ORIGIN      
        1 cggtccgggc gggcccaagg cgcgcgccgg cggcgaccac gagggctcgg agacccgcgg
       61 cgcggtcgag ggcagccggc gtggctcggc gctcggcgtg gagggcggca gcgagggtgg
      121 ccggttcggc ggcgagggca aaccaggtga tgacggcgtg acgatgggcg gcgcaatcgc
      181 cggtggtgtg atcgcggtcc ccgaggcgat caagggcgcc gtcgacgtcc tgctcatcgc
      241 ggacgcgggc gacgtcagcg gtgcgggcag tcagttattc ggccagctcg gcaaggagat
      301 cggcaagatg tctgccgcgg cgctccggca ggtggtggcg cgcgaggcac gggccgcggt
      361 cgagcacgag ctgcgcgcga cgatcgagag gatggcgcgc acgccgcggt ggcaggcgct
      421 caccgccgag gagcgggagc gcgccgtgcg gatcgcatac tacgagctgc agcggcgatt
      481 cttccgtggc tttggcgaca ccgcgaaggc tgcagaccgc tcggcgacga aggcgctgcg
      541 cggcgcgacc ggcgcgcgga agatcgccgc gcaggagagc ctcgacgcag cgcgggtcgg
      601 tgcggaggcg gccgagatcg agccggtcgc gggccggtta ccgcgcaacc atcgatacgc
      661 cggcaaggag tatccgcgcg agctcttgcc gccgaagtac cgggagaagg gcctgcggtt
      721 caagccgtcg ggccaccccg acttcgagcc gtacgcgctc accttgccga acgggcgcaa
      781 gacggtgaag atcgagctca ccggatcgcg gcgagccgac gaggcgctgg cgaacaaggc
      841 ggcggggttc aagggagagc cgccgagcac ccacgcgtgg caccacgtcg aggacgaggg
      901 tacgatgatg ctcgtaccga aggatctgca ccgagaggtt ccgcacacgg gcggcagggc
      961 cacgttcaag caccgcacag gagttgacta tggggactag ctgggcaccg ttctacgaga
     1021 ccgggccgcg ggtcaccgag gacgacgtcc gccggttcga gcgcgaattc ggccacgaac
     1081 tgccggccga ctatcgcgcg ttcctggtcg aggtgaacgg cggccacgtg cggcgcagcc
     1141 acagcgtgtt cacgttgcgg cgcggtgcgc ataaggacga gagcgtcctg aggggcctgt
     1201 tcagcctcaa cgctcccgac gaccaagacg atctcgcgac ggcgcagaag cactacaacc
     1261 ccgacgtcaa gctgcccgag ggattcctcg agatcggcta cgacggcttc ggctgccccg
     1321 ttgtcctgcc gctggtcggt cagcaccgcg gagaggtctg gtacttggac atcgaggacg
     1381 acggcgagga catcgaggac gacagcgagg accggccgag cgttcgcgtc gagtggttcg
     1441 atcggcgcga cgtctggaag ctcgccgaca gcttcgccga gttcatggcc gggctgcgtc
     1501 cgctcgagga cgacgccagc gcgagcgcat cgccctgacg cgccgagcag cgcgacgagc
     1561 gcggcgctga cgacgtcgac cgcttcgccc tcgatctccg acgaccagct ccggagtgac
     1621 ctggcccccg cgctcgactt gcgacgcgtg cctgcgacgc acggaccgcg caggaagccg
     1681 gtcgctggcg gatgctcgcc gcgcgatcct gaggcaggat gcacgcgaca tgcacccgat
     1741 ccggatcgcc cttccgctcg tgctcctcgg attgctcgcc gccgccgcgg ccgccgatcc
     1801 ggtcccggag gcgtcgtacc agcagctgca ctggcgcatg gtgggcccgt tccgcggcgg
     1861 gcggacccgc gcggtcgccg gcgtcccgtc gcagccgagc gtgttctaca tcgcacaggt
     1921 caacggcggg gtgtggaaga ccgacgacta cggccagacc tggcggccga tcttcgacga
     1981 gcaaccgacc cagtcgatcg gatcgatcgc ggtcgcaccg tcggatccga agatcatcta
     2041 cgtcggcagc ggcgagggcc tgcaccggcc ggacctgtcg gtcggcaacg gcgtctacaa
     2101 gtcgacggac gcgggcaaga cctggaccca cctcggcctc gacgacgccc agcagatccc
     2161 gcagatcgcc gtcgatccgc gcaaccccga tcgggtgtac gcggcggtgc tcggccaccc
     2221 ctacggcccg agcgagcagc gcggcgtgtt ccgctcgctc gacggcggca agacctgggc
     2281 ccgcgtgctg tacaaggacg ccaacaccgg cgcgtcggac ctcgagatcg atcccaggca
     2341 ccccgacgtg ctgtacgcgg cgctgtggga gtcgcggctc ggccccgccg aggacggcaa
     2401 cgagttccgc ggcaccggcg gcgggctcta caagtcgacc gacggcggca cgacgtggcg
     2461 caagctcgag ggcggcggcc tgcccgccaa cggcgtgcag ttcgacctcg cgatcgcccc
     2521 gagccagccc agccggctgt actgcgagct gagcaccacc gagcccggcg cctacggcac
     2581 ctccaggggc gccggcctgt accgctccga cgacgcgggc gcgacctgga cccggatcac
     2641 ccatgacgac cggccggcga tgaagatcgg cggcggcgat ctgatggtgc cggtggtcga
     2701 ccccaggaac ccggacgtcg tctacgtcgc gagcatcgtc gcgatgaagt cgaccgacgg
     2761 cggcaagacc tggacctggc tgcgcggcgc gcccggcggc gacgactacc agaacctgtg
     2821 gatcaacccg aacgatccaa aggtgttcgc cctggtcagc gaccagggcg ccaacatcac
     2881 ggtcaacggc ggcaagacct ggagcagctg gtacaaccag ccgaccgcgc agctctacca
     2941 cgtcggggtc accgccgatt tcccgtaccg gatctgcagc ggccagcagg agagcggctc
     3001 ggtgtgcatc tcgagccgcg gcaacgacgg ctcgatcgga taccgcgagt ggcggccggt
     3061 cgggatcatc gagtacggct acgccgcgcc cgatccgcgc aaccccgaca tcgtgtacgg
     3121 cgccggccgc aacgaggtct cgcgctacca gtggtcgacc ggccgggtcc agaacgtcac
     3181 gccgatcccg atccgcggca gctaccgcac cgagcgcacc cagccgatcg tgttctcgcc
     3241 gctgcgcccc agcgtcctgt actacgcgtc gagcgtgctg ttcgagtccg ccgacggcgg
     3301 ccagagctgg cgcacgatca gcccggatct cggccatccc caccccggtg tcccgccgag
     3361 cgtcggcgcg ctggcggcga gcaacaaggc cgccgaggac gcgcgcggcg cgatctacgc
     3421 gctggcgccg tcgttcaagg cgccgggcac gatctgggcc ggcaccgacg acggcaagct
     3481 gtgggtcacg cgcgacggcg gcgggcactg gaccgacatc acgccgcccg agctcacgcc
     3541 gtggagcaag gtgacccagc tcgaggcgtc gcacttcgac gacgtcaccg cctacgcctc
     3601 ggtcagccgg ttccggatcg acgacctcgc gccgtacatc taccggaccc acgacggcgg
     3661 caagacctgg acgctgatca cgcacggcct gccgcccggt ccggtcgacg ccgtgcgcga
     3721 ggacccggtc cgcaaggggc tgctctacgc gagcaccgag aacggcgtgt gggtgtcgtt
     3781 cgacgacggc gcggcctggc agtcgctgca gcgcaacctg ccgcacacct cggtgcgcga
     3841 cctggtcgtc cacgacagcg atctgatcgt cgcgacccac ggccgcgggt tctggatcat
     3901 ggacgacgtc agcgcgctgc gccagatcgc ggccgatccc aggaccgcca tgtccgacac
     3961 gctgttcaag cccgcgccgg cctaccgcgt cccgcacagc ctctacaccg acacgccggt
     4021 gccgcccgac gagccgctcg ccgagaatcc accgaccggc gcggtgatcg actactacct
     4081 ggcgcagccg gcgcaggggc cggtgatgct ggagatcagc gacgcgcgcg gccagctcgt
     4141 gcgccgctac gccagcaccg atccgccgga cctgaccgcc ggcgagatcg cgcgccagct
     4201 gatcccgtcg tactgggtgc ggccgcaccg ctcgctcggc accaccgccg gcgcccaccg
     4261 ctgggtctgg gacctgcgcg gcgcgcgccc gctcgcgacc tcgtacagct acccgatcag
     4321 cgcggtgccg ctcgacacgg tgcgtacccc gctcgggccc tgggtcctgc ccggcggcta
     4381 caccgtcaag ctgaccgtcc acggcaagac gctgagcgcg ccgctcgagg tccggctcga
     4441 tccgcggatc aagctcgcgc cggcggcgct cgcccagcgg caccagctcg cgctgcggct
     4501 gtccgatctg gtgagccgca gctcgcagct ggtgctccat gcgcagtcga ccgtcgatca
     4561 gctcaccagg ctcgccggca agccgccggc cgcgcagaag gacccgatcg aggcgacgct
     4621 ggccagggtc acggcggtgc tgtcggggcc caagggcccg ccgcccgctc cgggtcgcga
     4681 gcgcccgccc agcctgaccg gcgtcaacgg caagctggtc acgctgtacc ggatggtcga
     4741 cgtcgacgcc gcgccgaccg cggttcaggg caccgagacc gcgaaggccg agcgcgagct
     4801 gacgcagctc gccgccgcct gggatggggt ccggaccggc gagctggccg cgctcaacgc
     4861 cgcgctgacc gcggcgggac agccggcgat ccggcccgag ctggcgcccc agacccagca
     4921 ggacgagggc gacgaagagt gagcccgcgt atgggccctc tcacgggcat cgcgcccacc
     4981 gatcatgggt ggaacgatgt gccgagggcc ggtacgctgg ggattgcagg acacggatgt
     5041 cagcgcgcgt cgtctgtttc gccgtcgccg tcgccatcga gatcggagct gcgcagcacg
     5101 cgcacgcgct cgacggccac cgccgcgtca cgcagtacgc ccagacccac ttcgccgcgc
     5161 gcgatggcat gccccacggc gtcaccaccg cgatcgtcca gaccgacgat ggctacctgt
     5221 ggacggcgag ccacgaaggc ctgtcgcggt tcgacggggc cgcgttcacc aacttcgatc
     5281 atcgcgggac cgagggagtc ccgagcaaca cgttcaccgc gctcgcggtc gacgccgcgg
     5341 gcatgctgtg ggcagggacc cgcgatcacg ggttgctgca cctggtcggc ggcgagttcc
     5401 aggcggtggt atgggagccg gggccccaga accagcagat ccgcgcgctg gcgttcgacg
     5461 gcaacggcga tctgtgggtc ggcacgcgcg atcgcggtgt ggtccggctg cacgccggtg
     5521 tgctgacggc tgcgctgacc acccaggatg gtctccccag cgacgacatc cgcgcgctgt
     5581 atcgcacccg ggatggcgcg atctggatcg gcacgtttca cgggctggtg cagtggaagg
     5641 ccggccagct ggtgcgcggc cccgccgcgc tcgacggcac caccgtccac tcgatcgcgc
     5701 agggcccgcg cggcgatctg tggtgcgcca ccgacaaggg gctcgcccac ctccacgacg
     5761 acgtcgtcga catcctcgac gagaagcgcc tgtcggcgac cgacatccag agcgtgctgt
     5821 tcgaccgcga tggcaacctg tggttcggga ccggggccgg gctcgcgcgg atgacgttcg
     5881 gcgctgcggg ggttcagggc gggatccaga tcgagcggct gccgagcccc gacgtgctga
     5941 tcctcgcgct gttcgaggac tccgacggca acctgtggat cggcagcgag cgcgggctgg
     6001 accggctgcg cgacggcgac gcgctcccgt tcggcgcgag cgagggcttg accgacgacg
     6061 tcgtcctcgg gttccgcgag gatcccaccg gcgcgcgctg gctcaccacc agcagcgggc
     6121 tgtaccgggt cgagcccggc cagaccacgg cgacccggct cgtcgcggat cacgtgatgt
     6181 acgcgatcct tcccgactcg cacggcgacg tgtggttcgg cggccgcaac ggcgacgtcg
     6241 ggcgctggca cgacggtcag ttcacctggc tgggcaaggc gcgctgggag cgcgtgcgcg
     6301 cgatccgcga gaccgtcgac ggcatgtggt tcggcaccga ccacggcctg ttccggatgc
     6361 gcggcgagcg actgagcgac gccgaggtcg tggtggaggg gattgcggtc cgcgggatcg
     6421 tccccgacag cgacgggtcg ctgtggctcg cgaccgagaa cagcggcctg ctgcgctggc
     6481 gcgcgggcgc gcgggccgag atcccgtcgg ggggaccgcc gcgcgacatc cccaccgcca
     6541 cgatcacgtt cgactccgac ggcacgatgt gggtcggcac cgagggcggc gggctgtggc
     6601 ggctgcgcga tggccggtgg gtggccttca ccaccaagga cggcatgttc gacgacgtgg
     6661 tgtggcggat cctggacgac ggcctcggca acctgtggat gtcgtcgaac cgcgggatct
     6721 ggcgggtcag ccgcgaccag ctcgagaccc gcgcggtggg gctgcgtccc acgatcgact
     6781 cggcggtcta cggcgaggcc gacgggatgc gcgaccgcga gtgcaacggc gcgaacgact
     6841 ccgcgggctg gcggacccac gacggctggc tgtggttccc gaccggcaag ggcctggtcg
     6901 cgatcgatcc ggcgcacctg cggccgggac gaccgtcgcg cgcggtggtc gagaccgtgc
     6961 gcatcgacgg ccagccgcag cgggtgaccg accggctggt gctccccgcc gacaccggcc
     7021 ggctcgagct cggctacacc gccccgctgc tgcgcgggcc gcagcggctc cggttccgct
     7081 accagctcga gagcttcgac cggggctgga acgacgccgg gccgcagcgg accgcccagt
     7141 acaccaacct acctccgggc gactaccggt tccgggtcga ggccgggctc gatggcgagt
     7201 ggggcaagcc ggcgacgctc cgcgtgacgc tgccgccgcg gttctatcag aacgcctggt
     7261 tctacgtgct ggcgatcctg tcggtcgcgc tggcgatcgt cgcggtgccc ctgctgcgcg
     7321 tccgccagct ccgggcccgc gcccgcgagc tcgaccgccg cgtccaggag gcgatcagcg
     7381 agctcaaggt gctgtccggc ctcttgccga tctgcgcgtg gtgcaagaag gtccgcgacg
     7441 accacggcta ctggaaccgg atcgaggcct acctcagcgc gcggaccgac gcgcagttca
     7501 cccatgggat ctgccccgac tgcacgaaga aggtgatggc ggaagacagc gctctcgcaa
     7561 gccagagcgt cacgtccgtc aagcgcccgt gacacactcg accaaccccg atagagtccg
     7621 gggccgtgaa gcttcgatcc tcgccggccg cgtccctcat cgcgtcgctg gtcgcgtcct
     7681 tcatcgtcct cggcgtgacg gcctcgtgcg gcggcgcggg caagccaccg ccgaacccgc
     7741 cgacctcgtc tgccgccgat gacacgccgg tggtcatcg
//
LOCUS       DASVOR010000127         6010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_118201,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000127 DASVOR010000000
VERSION     DASVOR010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118201"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..455)
                     /locus_tag="VF516_02675"
     CDS             complement(234..455)
                     /locus_tag="VF516_02675"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8106599.1"
                     /translation="MPRDAIHRHIAKQIRSLAKQQGWSGNRLADTAGVSRAQLSRLLT
                     LQTSPTIGLLKKIAAALDVDTRDLLPPAR"
     gene            complement(1063..>6010)
                     /locus_tag="VF516_02680"
     CDS             complement(1063..>6010)
                     /locus_tag="VF516_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8106600.1"
                     /translation="ARLVGTEGTLGVTADVKDVSGIWKELTDNVNQMAKNLTNQVRDI
                     AEVTTAVANGDLTRKITVEARGELVTLKNTINTMVDQLNAFASEVTRVAREVGTEGVL
                     GVQAQVRDVSGIWKELTDNVNSMASNLTNQVRNIADVARAVAAGDLSRKITVDARGEV
                     LDLKATLNTMVDQLNSFASEVTRVAREVGTEGRLGGQASVEGVRGVWKELTDNVNGMA
                     SNLTNQVRDIAEVTTSVARGDLTRKITVDAKGEILELKNTVNTMVDQLSSFADQVTRL
                     ARDVGIEGRLGSQADVRGVLGTWRNLTDAVNSMAANLTDQVRNIAKVATAIANGDLQQ
                     KIVVDARGEILELKNTINTMVDQLSAFADQVTRVAREVGTEGKLGGQASVEGVRGTWK
                     ELTDNVNQMASNLTNQVRDISAVATAIARGDMTRKISVEVRGELLDLKNVINTMVDTL
                     SAWTDEVTRVAREVGVEGKLGGQAAVRGVAGTWKELTDNVNQMASNLTNQVRDIAEVT
                     TAVANGDLTRKITVEVRGELLQLKNTINTMVDQLGSFASEVTRVAREVGLDGVLGGQA
                     QVRGVSGTWRELTDNVNVMARNLTEQVRGIATVVTAVANGDLERKLTLTARGELATLV
                     DTINSMIDTLSTFAEQVTGVARDVGVEGSLGGQADVPGAAGVWRDLTNNVNELAGNLT
                     SQVRAIRDVATAVTQGDLTRSITVEARGEMAQLKDTVNQMIRTLAETTKVNQEQDWLK
                     TNVARFTRMLQGQRDLLGVARQVLNELAPLVNAHHGAFYMTEPNDDGKQQQLRLFASY
                     AYQERKSVSNVWRFGEGLVGQAALEGKRIVLSKVPSDYIQISSALGEAPPHAIVVVPI
                     PFENEVKGVIELASFERFSGIQLAFLDQMLESLGIVIATIEATMRTDELLRQSQSLTD
                     ELRNQQKQLRQTNEELEEQAVQLSAQKAEVELKNREVELARQELAERAEQLAVTSKYK
                     SEFLANMSHELRTPLNSLLILSRQLADNGEGNLTDKQIRYADTIRQAGVDLMTLINEI
                     LDLARIESGKMTVEISQVRFSNLRDYVEQTFQQVAAEKGLALSVELDGGLPSSMDTDD
                     MRLRQVLRNLLSNAIKFTERGKVRLRIFRAGPEVIGFAVADTGIGISRDKHQLIFEAF
                     QQADGSTSRKYGGTGLGLAISREIAGLLGGELRVESTVGAGSTFTLLLPTVYEAKSRE
                     RRMTAELPVAPEVIDVSPLVVPGEPPPSSPEAAPVAALTVRSIPDDYEHLEPGDRVLL
                     VIEDDLTFAATLLEMGRQSGFKAVVATSGMKALELSRTVKPDAITLDLRLPDIDGWVL
                     LDRLKHDPATRHIPVHVISGLDEERRSLQYGALGFLQKPVSTESLQAGLSGIHDFVEK
                     RVKNLLVIEDDPVQSQAITELIGDSDVATTSVPSAENALISLQENSYDCVVMDLRLPG
                     MSGFELIERIKADPRHRRLPVIVYTGRELTDEDKQRLHGLAQTVIIKDVTTMERLLDE
                     TALFLHRVEANLPERKQRVLRRLAKSDPQLAERNVLVVDDDLRNIFALTSLLEGHKMH
                     VQYAENGRRALTKLEDNTNIDIVLMDIMMPEMDGYEALRKIRERAEWKNLPVIALTAK
                     AMQGDREKCLEAGASDYITKPVDADQLLSLLRVWLYQA"
BASE COUNT          824 a         2033 c         2074 g         1079 t
ORIGIN      
        1 cagactagta gatcgcctga gccgttctca ggccccctgg tatggcggcg gccgtggagg
       61 gcacctggcg ggtggagctg acctgtcgca aagtgcggca gtacagctgc gacagtgccc
      121 gtgctgcaag cgcgtaccag ccagcgccac gcctgaagcc aggcctgacg ccacgacgga
      181 cgctcggctg gctgcacggc ctcgtcagcg acccgccggg ccgctctcgc tcgctatctg
      241 gccggtggca acagatcgcg cgtgtcgaca tcgagcgcgg ccgcgatctt cttcaagagg
      301 ccgatcgtcg gcgacgtctg cagggtgagc agccgcgaca gctgcgcgcg gctcaccccc
      361 gcggtgtctg caagccggtt cccgctccag ccctgctgct tcgcgagcga ccgaatctgt
      421 ttggcgatgt gtcggtgaat ggcgtccctg ggcacagccc tacgatattg agttttggta
      481 ccgcttcaag tcctgtaacg cgattcctct tatggaacag tcagtgcagt agaactgcgc
      541 tcatcgaaga tttgaaccgc cgacgccgtc gaggctgctg agcgacgacg gtgatcgcat
      601 ccgccgccga gccgctgcgc acagcggtgg cgagatgatg cgcatagcgc aacacgctgg
      661 cagcttccac gccgccgcat attgctgcgg tcggcattaa ataattacag actcgtagat
      721 cgcctgagcc gttctcaggc agcctggtat gaccgaagcc ggcgagggca cctggcgggt
      781 ggagccctgt cgcaaattgc ggcagtacag ctgcgacact gcgcgtgctg caatgcagga
      841 ccgcattcca cttccggacg acgcgggtcg ccgcacagtt ttctgcgacg tgggcggcgg
      901 cgggggaagc ccggccggcc gggagcgcat cgggcggggc cgcggaattg agtacgccag
      961 agcacgttcg tggacggcgg cgtaccggac acgggacacc cccccggcgg cgagatgccc
     1021 cgcgcgggac acccgctcgc agtaggtgaa gatcaggtca ggtcaggcct ggtacagcca
     1081 gacccggagc agcgacagca gctggtcggc atcgaccggc ttggtgatgt agtccgacgc
     1141 gccggcctcc aggcacttct cgcgatcgcc ctgcatcgcc ttggcggtca gcgcgatcac
     1201 cgggaggttc ttccactcgg cgcgctcgcg gatcttgcgc agcgcctcgt agccgtccat
     1261 ctcgggcatc atgatgtcca tcaggacgat gtcgatgttg gtgttgtcct cgagcttggt
     1321 cagcgcgcgc cggccgttct cggcgtactg gacgtgcatc ttgtggccct cgagcagcga
     1381 ggtcagcgcg aagatgttgc gcaggtcgtc atcgacgacc agcacgttgc gctcggcgag
     1441 ctgaggatcg ctcttggcga gccggcgcag cacgcgctgc ttgcgctcgg gcaggttggc
     1501 ctcgacccgg tgcaggaaca gcgcggtctc gtcgagcagc cgctccatgg tcgtgacgtc
     1561 cttgatgatc acggtctggg ccaggccgtg cagccgctgc ttgtcctcgt cggtcagctc
     1621 gcggccggtg tagacgatca ccggcagccg gcggtgccgc gggtcggcct tgatccgctc
     1681 gatcagctcg aagccggaca tcccgggcag ccggaggtcc atcacgacgc agtcgtagct
     1741 gttctcctgc agcgagatca gcgcgttctc ggccgacggc acgctggtgg tcgcgacgtc
     1801 gctgtcgccg atcagctcgg tgatcgcctg gctctgcacc gggtcgtcct cgatcacgag
     1861 caggttcttg acccgcttct cgacgaagtc gtggatcccg gacagcccgg cctgcaggct
     1921 ctcggtgctg accggcttct gcaggaagcc gagcgcgccg tactgcaggc tgcggcgctc
     1981 ctcgtcgagc ccggagatca cgtggaccgg gatgtggcgg gtcgccggat cgtgcttgag
     2041 ccggtcgagc agcacccagc cgtcgatgtc gggcagccgg aggtcgagcg tgatcgcgtc
     2101 cggcttgacc gtgcgggaca gctcgagcgc cttcatcccg ctggtcgcga ccaccgcctt
     2161 gaacccggac tggcgcccca tctcgagcag cgtcgcggcg aacgtcaggt cgtcctcgat
     2221 caccagcagc acgcggtcgc cgggctcgag gtgctcgtag tcgtcgggga tcgatcgcac
     2281 ggtcagcgcg gccaccggcg ccgcctccgg gctggagggc ggcggctcgc cggggaccac
     2341 gagcggcgag acgtcgatca cctcgggcgc caccggcagc tcggcggtca tccgccgctc
     2401 gcgcgacttg gcctcgtaca cggtcggcag cagcagcgtg aacgtgctgc cggccccgac
     2461 cgtgctctcg acccgcagct cgccgccgag cagcccggcg atctcgcgcg agatcgccag
     2521 gccgagcccg gtgccgccgt acttgcggct ggtcgagccg tcggcctgct ggaaggcctc
     2581 gaagatcagc tggtgcttgt cgcgcgagat cccgatcccg gtgtccgcca ccgcgaaccc
     2641 gatcacctcg ggccccgcgc ggaagatccg cagcctgacc ttgccgcgct cggtgaactt
     2701 gatcgcgttg gacagcaggt tgcgcagcac ctggcgcagc cgcatgtcgt cggtgtccat
     2761 cgacgacggc agcccgccgt cgagctcgac cgacagcgcc aggcccttct ccgcggcgac
     2821 ctgctggaag gtctgctcga cgtagtcgcg caggttgctg aaccggacct gcgagatctc
     2881 gaccgtcatc ttgccggact cgatccgggc gaggtcgagg atctcgttga tcagcgtcat
     2941 caggtcgacg ccggcctggc ggatcgtgtc ggcgtatcgg atctgcttgt cggtcaggtt
     3001 gccctcgccg ttgtcggcga gctggcgcga caggatcagc agcgagttga gcggcgtgcg
     3061 cagctcgtgc gacatgttgg cgaggaactc ggacttgtac ttcgaggtca ccgcgagctg
     3121 ctcggcgcgc tcggcgagct cctggcgggc gagctcgacc tcgcggttct tgagctcgac
     3181 ctcggccttc tgcgccgaca gctggaccgc ctgctcctcg agctcctcgt tggtctggcg
     3241 cagctgcttc tgctggttgc gcagctcgtc ggtcaggctc tgcgactggc ggagcagctc
     3301 gtcggtccgc atcgtcgcct cgatcgtcgc gatcacgatg ccgagcgact cgagcatctg
     3361 gtcgaggaac gcgagctgga tcccggagaa ccgctcgaac gacgccagct cgatcacgcc
     3421 cttgacctcg ttctcgaacg ggatcggcac cacgacgatc gcgtgcggcg gggcctcgcc
     3481 gagcgccgac gagatctgga tgtagtccga cggcaccttg gacagcacga tccgcttgcc
     3541 ctcgagcgcg gcctggccga ccaggccctc gccgaaccgc cacacgttgg acaccgactt
     3601 gcgctcctgg taggcgtacg acgcgaacag ccggagctgc tgctgcttgc cgtcgtcgtt
     3661 gggctcggtc atgtagaacg cgccgtggtg ggcgttgacc agcggcgcga gctcgttgag
     3721 cacctggcgc gcgacgccga gcaggtcgcg ctggccctgg agcatccggg tgaaccgcgc
     3781 gacgttggtc ttgagccagt cctgctcctg gttgaccttg gtggtctccg ccagggtccg
     3841 gatcatctgg ttgacggtgt ccttgagctg ggccatctcg ccgcgcgcct cgaccgtgat
     3901 cgaccgcgtc aggtcgccct gggtcaccgc ggtcgcgacg tcgcggatcg cgcggacctg
     3961 gctggtcagg ttgccggcga gctcgttgac gttgttggtc agatcgcgcc acacgccggc
     4021 ggcgcccggc acgtcggcct ggccgccgag gctgccctcg acgccgacgt cgcgcgccac
     4081 gccggtgacc tgctcggcga acgtcgacag cgtgtcgatc atgctgttga tcgtgtcgac
     4141 cagggtcgcg agctcaccgc gcgcggtcag cgtcagcttg cgctcgaggt cgccgttggc
     4201 gaccgcggtg accacggtcg cgatgccgcg gacctgctcg gtcaggttgc gcgccatgac
     4261 gttgacgttg tcggtcagct cgcgccaggt cccggacacg ccgcggacct gggcctggcc
     4321 gccgagcacg ccgtcgaggc cgacctcgcg cgcgacccgg gtgacctcgg acgcgaacga
     4381 gccgagctgg tcgaccatcg tgttgatcgt gttcttgagc tggagcagct cgccgcggac
     4441 ctcgacggtg atcttgcggg tcagatcgcc gttggcgacc gcggtggtga cctcggcgat
     4501 gtcgcggacc tggttggtca ggttcgacgc catctggttg acgttgtcgg tcagctcctt
     4561 ccaggtgccg gcgacgccgc gcaccgcggc ctggccgccg agcttgccct cgacgccgac
     4621 ctcgcgcgcg acccgggtga cctcgtcggt ccacgccgac agcgtgtcga ccatcgtgtt
     4681 gatcacgttc ttgaggtcga gcagctcgcc gcggacctcg accgagatct tgcgggtcat
     4741 gtcgccgcgc gcgatcgcgg tcgcgaccgc cgagatgtcg cggacctggt tggtcaggtt
     4801 cgacgccatc tggttgacgt tgtcggtcag ctccttccag gtgccgcgca cgccctcgac
     4861 cgaggcctgg ccgccgagct tgccctcggt gccgacctcg cgggcgaccc gggtcacctg
     4921 gtcggcgaac gccgacagct gatcgaccat ggtgttgatc gtgttcttga gctcgaggat
     4981 ctcgcccctg gcgtcgacca cgatcttctg ctggaggtcg ccgttggcga tcgcggtcgc
     5041 gaccttggcg atgttgcgga cctgatcggt caggttggcg gccatcgagt tgaccgcgtc
     5101 ggtcaggttg cgccaggtgc cgagcacgcc gcggacgtcg gcctggctgc cgagccggcc
     5161 ctcgatcccg acgtcgcgcg ccagccgggt gacctggtcg gcgaacgagc tcagctggtc
     5221 gaccatggtg ttgaccgtgt tcttgagctc gaggatctcg cccttggcgt cgaccgtgat
     5281 cttgcgggtc aggtcgccgc gcgcgaccga cgtggtgacc tcggcgatgt cgcggacctg
     5341 gttggtcagg ttcgacgcca tgccgttgac gttgtcggtc agctccttcc acaccccgcg
     5401 cacgccctcg accgaggcct ggccgccgag ccggccctcg gtgccgacct cgcgggcgac
     5461 ccgggtgacc tcgctcgcga agctgttgag ctgatcgacc atcgtgttga gcgtggcctt
     5521 gaggtcgagg acttccccgc gcgcgtcgac cgtgatcttg cgcgacaggt cgccggccgc
     5581 gaccgcgcgc gcgacgtcgg cgatgttgcg gacctggttg gtcaggttcg acgccatcga
     5641 gttgacgttg tcggtcagct ccttccagat ccccgagacg tcgcggacct gggcctggac
     5701 gccgagcacg ccctcggtgc cgacctcgcg ggcgacgcgg gtcacctcgg acgcgaacgc
     5761 gttcagctga tcgaccatcg tgttgatcgt gttcttcagg gtgaccagct cgccacgtgc
     5821 ttcgaccgtg atcttgcggg tcagatcgcc gttcgcgacg gcggtcgtca cctcggcgat
     5881 gtcgcggacc tggttggtca ggttcttggc catctggttg acgttgtcgg tcagctcctt
     5941 ccagatcccc gacacgtcct tgacgtcggc ggtgacgccc agcgtgccct cggtgccgac
     6001 caggcgggcg
//
LOCUS       DASVOR010000128         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410424,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000128 DASVOR010000000
VERSION     DASVOR010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410424"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..829)
                     /locus_tag="VF516_02685"
     CDS             complement(14..829)
                     /locus_tag="VF516_02685"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8106601.1"
                     /translation="MTSRYLVCAVLVVAACKGSAQKEEGAKAKADPLEAVADRSLPGN
                     PFAKSPQAVVQGKKLYMKNGCPGCHGVGGGGGMGKPLIDDEWRFGSDDPTLFKLIRGE
                     IPNQTMPNTFGKVLTEDEVWKILTYVRSIYAGDPTKINWTVPPPVPPELAALADKPEG
                     DPIAAGKQTFLTICITCHGPEGKGDGPASATLNPKPRNLTDASYMSQINDRYLFELIS
                     RGGVAVGKSPLMPSQPTLTAQDLNNVIAYVRSLSGTAASGGNTAGTGIVRTGH"
     gene            complement(855..1715)
                     /locus_tag="VF516_02690"
     CDS             complement(855..1715)
                     /locus_tag="VF516_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8106602.1"
                     /translation="MTNHVLASSLAFLAIGSVLLCAPGRASADAATPARPAKELRVCG
                     DPNNLPFSNEKGEGIENKIAEVIAKDLGETVVYFWWPHQRGVVKRVLNAKHCDVMLGI
                     PKGYDPVLWTRPYYRTGYVMVYRKDRKLKLRSLDDPALKTLKVGVEVNTPPHDALGRR
                     GIADNIVGYQLMFDSNFHAEEYLGKEVEDLIAGNLDVAMVWGPIAGYFAKKRGAPLEI
                     VPIDDPSDRGARFAFDISMGVRKGDKELKEQLEAALARRHDEIEHVLADFGVPLLPAA
                     EEKAHDKGAR"
     gene            complement(1743..>2649)
                     /locus_tag="VF516_02695"
     CDS             complement(1743..>2649)
                     /locus_tag="VF516_02695"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8106603.1"
                     /translation="TIWARNPDTGKAKWGYQMTPHDAWDYDGVNESILADLTIGGKKV
                     PALVHFDRNGFGYTMDRTNGTLLVAQPFAAVNWAKSIDLKTGRPVEDSSKRTKQGQVT
                     KDICPNAMGGKDQQPAAFSPVTGLFYVPTNNMCMDYEATEVKYTAGAPYVGANVLMKP
                     GPGDGHLGDLIAWDATTGKKVWDIPEKQPCWGGVLTTKTGLVFYGTMDGWFKAVDAKN
                     GKLLWKHKVGSGIIGSPMTFQGPDKKQYVAVYSGVGGWYGAAVSLDLPPNDPYAALGE
                     VGIAYKSGLDKATTKGGAVYVFSLE"
BASE COUNT          387 a          918 c          850 g          494 t
ORIGIN      
        1 ggcgcaccgt gcctcagtgc ccggtgcgga cgatgccggt gccggcggtg ttgccgcccg
       61 aggcggcggt gcccgacagc gaccgcacgt acgcgatcac gttgttgagg tcctgcgcgg
      121 tcagcgtggg ctgcgacggc atcagcggcg acttgccgac cgccactccg ccccggctga
      181 tcagctcgaa caggtagcgg tcgttgatct gcgacatgta cgacgcgtcg gtcaggttcc
      241 gcggcttggg attgagcgtc gcgctggccg gtccgtcgcc cttgccctcc gggccatgac
      301 acgtgatgca gatcgtcagg aaggtctgct tgccggcggc gatcggatcg ccctccggct
      361 tgtccgccag tgcggccagc tccggcggca ccggcggcgg caccgtccag ttgatcttgg
      421 tcgggtcgcc cgcatagatc gagcggacat aggtcaggat cttccagacc tcgtcttcgg
      481 tcagcacctt tccgaaggtg ttcggcatgg tctggttcgg gatctcgccg cggatcagct
      541 tgaacagcgt cggatcgtcg ctgccgaacc gccactcgtc gtcgatcagc ggcttgccca
      601 tgccgccgcc cccgccgacc ccgtgacagc ccggacagcc gttcttcatg tacagcttct
      661 tgccctgcac cacggcctgg ggactcttgg cgaacgggtt gcccggcaac gaccggtcgg
      721 cgaccgcctc gagcgggtcc gccttggcct tcgcgccttc ctccttctgc gcggagccct
      781 tgcaggcggc gacgacaagc accgcgcaaa ccagatacct ggatgtcatg acctgaacct
      841 ctggccgccg gcgatcagcg cgcacccttg tcgtgagcct tctcctcggc ggcagggagg
      901 agcggcacac cgaagtccgc caggacgtgt tcgatctcgt cgtggcgtcg ggcgagcgca
      961 gcctcgagct gttccttgag ctccttgtcg ccctttcgca cgcccatcga gatgtcgaac
     1021 gcgaaccggg ccccgcggtc ggacggatcg tcgatcggca cgatctccag tggcgcgccc
     1081 ctcttcttcg cgaagtaacc ggcgatcgga ccccacacca tcgcgacatc gaggttcccg
     1141 gcgatcaggt cctcgacctc cttgccgaga tactcctccg cgtggaagtt ggaatcgaac
     1201 atcagctggt agccgacgat gttgtcggcg atgccgcgcc gtccgagcgc gtcgtgcggc
     1261 ggcgtgttga cctccacccc gaccttgagc gtcttgagcg ccggatcgtc gagcgagcgc
     1321 agcttgagct tgcgatcctt gcgatagacc atgacgtatc cggtccggta atacggcctg
     1381 gtccacagca ccggatcgta gcccttgggg atgccgagca tgacatcgca gtgcttggcg
     1441 ttcagcaccc gcttgacgac cccgcgctgg tgcggccacc agaagtagac cacggtctcc
     1501 ccgagatcct tcgcgatcac ctcggcgatc ttgttctcga taccttcgcc cttctcgttc
     1561 gagaacggca ggttgttcgg atcgccgcag acccggagct ccttggccgg cctggccggc
     1621 gtcgccgcat cggccgacgc gcggccaggc gcgcagagga gcaccgagcc aatggccaga
     1681 aacgccagcg agctcgcgag gacgtggttc gtcatcgggt caccgtggca cggcgccagc
     1741 ggtcattcca gcgagaacac gtagacggcg ccacccttcg tggtggcctt gtcgaggccg
     1801 ctcttgtacg cgatgccgac ctccccgagc gccgcgtacg gatcgttggg cggcaggtcc
     1861 agggacaccg ccgcgccata ccagccgccg acgccggagt acaccgcgac gtactgcttc
     1921 ttgtccgggc cctggaaggt catcggggag ccgatgatgc cggagccgac cttgtgcttc
     1981 cacaacagct tcccgttctt ggcgtcaacc gccttgaacc agccatccat cgttccgtag
     2041 aacaccaggc cggtcttggt ggtcagcacg ccgccccagc acggctgctt ctccgggatg
     2101 tcccacacct tcttgccggt cgtcgcgtcc cacgcgatca ggtcgccgag gtggccgtcg
     2161 cccgggcccg gcttcatcag cacgttcgcg ccgacgtacg gcgcgcccgc ggtgtacttg
     2221 acctcggtgg cctcgtaatc catgcacatg ttgttggtcg ggacgtagaa cagcccggtg
     2281 accggcgaga acgcggcggg ctgctgatcc ttaccgccca tggcgttcgg gcagatgtcc
     2341 ttggtcacct ggccctgctt ggtccgcttg gacgaatcct cgaccggccg accggtcttc
     2401 aggtcgatgc tcttggccca gttgacggcg gcgaacggct gggcgaccag cagcgtgccg
     2461 ttggtgcgat ccatggtgta gccgaacccg ttgcggtcga agtggaccag cgccggaacc
     2521 ttcttgccgc cgatcgtgag gtccgcgagg atgctctcgt tgacgccgtc gtagtcccac
     2581 gcgtcgtgcg gcgtcatctg gtagccccac ttcgccttgc cggtgtcggg attgcgcgcc
     2641 cagatcgtc
//
LOCUS       DASVOR010000129         3417 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439523,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000129 DASVOR010000000
VERSION     DASVOR010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439523"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..3283
                     /locus_tag="VF516_02700"
     CDS             113..3283
                     /locus_tag="VF516_02700"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8106604.1"
                     /translation="MLAALAAAQAQAPERQIDTSRGPTSELYIRKRPPAPEAPVLSAE
                     LKTLLASTEKKRDDKRLEAIGLLRAFLDSKPTGESRAEGVFKLAELLWEESRRLYLIK
                     MDEFSRAIEACAQKQNSCEQPKEPRIDLGQAEALYLDLHEHHPDFRRMDLVTYLIGFA
                     EKEAQHEDAALGRFNEVIQRFPGSPLFGDAWMMVGEHFFSAAEWPKAMDAYRHIPDDA
                     ATSDLATFKIAWCEWKLGNAIQAAKDFKRVLDKAVAAERSGTEAQRRRSASLRDEALQ
                     YMVVVFTEDRSITPKEVFDFLVSIDGEQYSRDVMVKVAESYAAQAEWDRSNSAYRFLV
                     KMDPESIKAADYQRSIIANWNSALDLDHAQEEIGVLLASYGPNSAWAKAQRNREALAR
                     SLDTTEDLVRVTATNIHGEAQRRERALKRPEQKQCVTRPELPGEVLGLYTRAADAYAG
                     YLTAFGGKPAAEKTAEKATEKTAEKTTDKATEIRYYRADILCFKLGKVEAAGDEYLAV
                     GRSAPVGKYHRDALLNAMNAFELARPKDTQGRRQLYPVDKKFGEAIDLYATLFPADKT
                     LVGVIFKNGQMFYDYGEYDEAIKRFGVIVTKYPDDENAGPAGDRILSALNKAADYENI
                     ESWARKLKKAKSFSDKAQQDRLDRLIVESIQKSGDKYAEAGKYIEAAGFYTRVPKETG
                     DPKVAAQALTNAGVMYEKAKQPEKAADLYLDLAEKYGDKAPEIAEKAAFSAGQVYEKV
                     IYYDRAAKAYELVWTKFRTGTKAADALYNAALLRQALGQNKEAIAHYGEYAKKFRERK
                     DAPDVAFNIGVVYENAGDEGPAYAAFSDYARVYRSTGKRIVEAHTRAGRTALRLGQLK
                     KAKDDFAAAQKAWKAATGKEKKEATTWAAEARYHEGELIFREYEKVSLDVKPTLLEKT
                     LKTKGKLLAEAEKVYTSVIDYQDLKWATAALFRVGQVYDGFAEALATAASKPPASLTP
                     DQVQAYQDGINAYVVDIQDKAVTLYTAGYQKAIQMQVYDEYTAKIREALGRIAADKFP
                     PEKESRQRERIGDRPPTPELITEVAR"
BASE COUNT          659 a         1204 c         1152 g          402 t
ORIGIN      
        1 tgtggaacgc gagcatgatc ggcgacgacg aggagttctg gccctggcaa ggtgagtact
       61 ggtccgacga atacgagggc tggcgatgat cgagccaaca cgtagccacc gcatgctggc
      121 ggcgctcgcc gccgcccagg cccaggctcc cgagcgccag atcgacacgt cgcgcggccc
      181 gacctcggag ctctacatcc gcaagcggcc gcccgcgccc gaggccccgg tgctgtcggc
      241 cgagctcaag acgctgctgg cgtcgaccga gaagaagcgc gacgacaagc ggctcgaggc
      301 gatcggcctt ctgcgcgcgt tcctcgacag caagccgacc ggcgagtcgc gcgccgaggg
      361 cgtgttcaag ctcgccgagc tgctgtggga ggagtcgcgc cggctctacc tgatcaagat
      421 ggacgagttc tcgcgcgcga tcgaggcctg cgcgcagaag cagaacagct gcgagcagcc
      481 caaggagccg cggatcgatc tcggccaggc cgaggcgctg tacctcgacc tccacgagca
      541 ccaccccgac ttccggcgga tggacctggt gacctacctg atcgggttcg ccgagaagga
      601 ggcccagcac gaggacgccg cgctcggccg gttcaacgag gtgatccagc ggttcccggg
      661 ctcgccgctg ttcggcgacg cgtggatgat ggtcggcgag cacttcttct ccgccgccga
      721 gtggcccaag gcgatggacg cctaccgcca catccccgac gacgccgcga ccagcgacct
      781 cgcgacgttc aagatcgcgt ggtgcgagtg gaagctcggc aacgcgatcc aggccgccaa
      841 ggacttcaag cgcgtgctcg acaaggcggt cgccgccgag cgcagcggca ccgaggccca
      901 gcgccggcgc agcgcgtcgc tgcgcgacga ggccctgcag tacatggtcg tggtgttcac
      961 cgaggaccgc tcgatcacgc cgaaagaagt cttcgacttc ctggtgtcga tcgacggcga
     1021 acagtactcg cgcgacgtca tggtcaaggt cgccgagagc tacgccgcgc aggccgagtg
     1081 ggaccgctcg aactcggcct accggttcct ggtcaagatg gacccggagt cgatcaaggc
     1141 cgccgactac cagcgcagca tcatcgcgaa ctggaacagc gcgctcgatc tcgaccacgc
     1201 ccaggaggag atcggcgtcc tgctggcgag ctacgggccg aacagcgcgt gggccaaggc
     1261 gcagcgcaac cgcgaggcgc tcgcgcgctc gctcgacacc accgaggacc tggtgcgggt
     1321 caccgcgacc aacatccacg gcgaggccca gcgccgcgag cgcgcgctca agcgccccga
     1381 gcagaagcag tgcgtgaccc ggccggagct gccgggcgag gtgctggggc tctacacccg
     1441 cgccgccgac gcctacgccg ggtacctcac cgcgttcggc ggcaagcccg ccgccgagaa
     1501 gaccgccgag aaggccaccg agaagaccgc cgagaagacc accgacaagg cgaccgagat
     1561 ccgctactac cgcgccgaca tcctgtgctt caagctcggc aaggtcgagg ccgccggcga
     1621 cgagtacctc gcggtcggcc ggagcgcgcc ggtcggcaag taccaccgcg acgcgctgct
     1681 caacgcgatg aacgcgttcg agctcgcgcg gcccaaggac acccagggcc gccgccagct
     1741 ctacccggtc gacaagaagt tcggcgaggc gatcgacctc tacgcgacgc tgttccccgc
     1801 cgacaagacc ctggtcggcg tgatcttcaa gaacggccag atgttctacg actacggcga
     1861 gtacgacgag gcgatcaagc ggttcggcgt gatcgtcacc aagtaccccg acgacgagaa
     1921 cgccgggccg gccggcgatc gcatcctgtc ggcgctcaac aaggcagccg actacgagaa
     1981 catcgagagc tgggcgcgca agctcaagaa ggccaagtcg ttctccgaca aggcgcagca
     2041 ggaccggctc gaccggctga tcgtcgagtc gatccagaag agcggcgaca agtacgccga
     2101 ggccggcaag tacatcgagg ccgccgggtt ctacacccgc gtccccaagg agaccggcga
     2161 tcccaaggtc gcggcccagg cgctgaccaa cgccggcgtg atgtacgaga aggccaagca
     2221 gcccgagaag gccgccgacc tctacctcga cctcgccgag aagtacggcg acaaggcgcc
     2281 cgagatcgcc gagaaggccg cgttctccgc cggccaggtc tacgagaagg tgatctatta
     2341 cgaccgcgcc gcgaaggcct acgagctggt gtggaccaag ttccgcaccg ggaccaaggc
     2401 ggccgacgcg ctgtacaacg ccgccctgct ccgccaggcg ctcggccaga acaaggaggc
     2461 gatcgcgcac tacggcgagt acgccaagaa gttccgcgag cgcaaggacg cgcccgacgt
     2521 cgcgttcaac atcggcgtgg tctacgagaa cgcgggcgac gaagggccgg cctacgccgc
     2581 gttctccgac tacgcgcggg tctaccgctc gaccggcaag cggatcgtcg aggcccacac
     2641 ccgggccggc cgcaccgcgc tccggctcgg ccagctcaag aaggccaagg acgacttcgc
     2701 cgccgcccag aaggcgtgga aggccgccac cggcaaggag aagaaggagg ccacgacgtg
     2761 ggccgccgag gcccgctacc acgagggcga gctgatcttc cgcgagtacg agaaggtgtc
     2821 gctcgacgtc aagccgaccc tgctcgagaa gacgctcaag accaagggca agctgctcgc
     2881 cgaggccgag aaggtctaca cctcggtgat cgactaccag gacctcaagt gggcgaccgc
     2941 cgcgctgttc cgcgtcggcc aggtctacga cggcttcgcc gaggcgctcg cgaccgcggc
     3001 cagcaagccg ccggcgagcc tgacccccga ccaggtccag gcctaccagg acggcatcaa
     3061 cgcctacgtg gtcgacatcc aggacaaggc cgtcacgctc tacaccgccg gctaccagaa
     3121 ggcgatccag atgcaggtct acgacgagta caccgcgaag atccgcgagg cgctcggccg
     3181 gatcgccgcc gacaagttcc cgcccgagaa ggagtcgcgg cagcgcgagc ggatcggcga
     3241 tcgcccgccg acccccgagc tgatcaccga ggtagcccga tgaccgaggg cgatctctgc
     3301 gggatgaccg ccacggcgat ggccacggcg gcgcggagct gcctcgcgct ggcgctcgcg
     3361 gcatgcgcgc tggtcgcctg cggcggcggc ggcggcggca ccaagggcac gaccccc
//
LOCUS       DASVOR010000130         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_322777,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000130 DASVOR010000000
VERSION     DASVOR010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322777"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1123
                     /locus_tag="VF516_02705"
     CDS             <1..1123
                     /locus_tag="VF516_02705"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8106605.1"
                     /translation="VRKQGLEHFIKFVRELPSPSPQAMFQALSDHGYRGQDAARRALC
                     LMAYRHIKRIKRIYIDGVDRTELPRKSNFLCVGPTGSGKTFLVETLFGKILKLPTALV
                     DITTYSETGYVGQDPSTILTRLLHTADENPMLASIGIVCLDEFDKIASGQNNAIFAGA
                     GTTKDVTGMGVQRELLKMLEASEVVVPLDLTHSSYADHVVMSTADIAFIAAGAFSGFQ
                     QIAHRRAMRDQIGFGRDQVPAGVDPDAIAVDFSAEQVENVANFQAYGFLPELVARFTR
                     VVPFRALDAATLKDILRSDVVQRMTREFRLEGFELTIDEAVLDHIVADALRRETGARG
                     LASTLTRQLEDVAFGAFGVEQGGAVTVKLSQGTIAVEIS"
     gene            1189..>1747
                     /locus_tag="VF516_02710"
     CDS             1189..>1747
                     /locus_tag="VF516_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8106606.1"
                     /translation="MDKIVVEGGARLTGRIPISGAKNAALPLLAAALLPGGASTYKNV
                     PRLADVATMAKLLRMLGWQVDGDGDSTELTIAPPTARSRLKLEAPYELVKTMRASVLV
                     LGPLVARYGRGRVSLPGGCAIGARPIDQHLKGLQAMGAKVTLDHGYVDVEARRLKGAT
                     IVLDVPTVTGCENLIMAAALAKGRTV"
BASE COUNT          268 a          627 c          595 g          257 t
ORIGIN      
        1 ggtccgcaag cagggcctag agcacttcat caagttcgtg cgcgagctgc cgtcgccgtc
       61 cccccaggcg atgttccagg cgctgtccga ccacggctac cgcggccagg acgcggcgcg
      121 ccgtgcgctg tgcctgatgg cgtaccgcca catcaagcgg atcaagcgga tctacatcga
      181 cggggtcgac cgcaccgagc tgccgcgcaa gtccaacttc ctgtgcgtcg gtccgaccgg
      241 gtcgggcaag acgttcctgg tcgagaccct gttcggcaag atcttgaagc tgccgaccgc
      301 gctggtcgac atcacgacgt actccgagac cggctatgtc ggccaggacc cctcgaccat
      361 cctgacccgc ctgctccaca ccgcggacga gaacccgatg ctggcgtcga tcggcatcgt
      421 ctgtctcgac gagttcgaca agatcgcgtc gggccagaac aacgcgatct tcgcaggcgc
      481 cggcaccacc aaggacgtca ccgggatggg cgtgcagcgc gagctgctca agatgctcga
      541 ggccagcgag gtcgtggtcc cgctcgatct gacccacagc tcgtacgccg accacgtcgt
      601 gatgtcgacc gccgacatcg cgttcatcgc cgccggcgcg ttctccgggt tccagcagat
      661 cgcccaccgc cgggcgatgc gcgaccagat cgggttcggc cgcgaccagg tgcccgccgg
      721 ggtcgatccc gacgcgatcg cggtcgactt ctccgccgag caggtcgaga acgtcgccaa
      781 cttccaggcc tacgggttct tgcccgagct cgtcgcgcgg ttcacccggg tcgtgccgtt
      841 ccgcgcgctc gacgccgcga cgctcaagga catcctgcgc agcgatgtcg tgcagcgcat
      901 gacccgcgag ttccggctcg agggcttcga gctgacgatc gacgaggccg tgctcgatca
      961 catcgtcgcg gacgcgctgc gccgcgagac cggcgcgcgc ggcctggcgt cgaccctgac
     1021 ccgccagctc gaggacgtcg cgttcggcgc gttcggcgtc gagcagggcg gcgcggtcac
     1081 cgtgaagctg tcgcagggca cgatcgccgt cgagatcagc tgaccggcgc aatagcggcc
     1141 cgccgcgcgg cgttctcccg gtggacgtgg tacgtccatc ggtgccgtat ggacaagatc
     1201 gtcgtcgagg gtggcgctcg cctgaccggc cggatcccga tcagcggggc caagaacgcc
     1261 gcgctgccgc tgctcgccgc cgccctgctg ccgggcgggg cgtcgaccta caagaacgtc
     1321 ccccggctcg ccgatgtcgc gaccatggcc aagctgctcc gcatgctcgg ctggcaggtc
     1381 gacggggacg gggacagcac ggagctgacg atcgcgccgc cgaccgcgag gtcccggctc
     1441 aagctcgagg ccccctacga gctggtcaag accatgcggg ccagcgtgct ggtgctcggc
     1501 ccgctggtcg cgcgctacgg ccgcggccgg gtgtcgctgc ccggcggctg cgcgatcggc
     1561 gctcggccga tcgaccagca cctcaagggc ctgcaggcga tgggcgccaa ggtgacactg
     1621 gaccacggct acgtcgacgt cgaggccaga cggctcaagg gcgcgacgat cgtgctcgac
     1681 gtcccgaccg tgaccggctg cgagaatctg atcatggccg ccgcgctcgc gaagggccgc
     1741 accgtga
//
LOCUS       DASVOR010000131         2753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205847,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000131 DASVOR010000000
VERSION     DASVOR010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205847"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2753)
                     /locus_tag="VF516_02715"
     CDS             complement(<1..>2753)
                     /locus_tag="VF516_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX8106607.1"
                     /translation="VGADVAARVEALGQATGSTLFMVLIAAFAVVLGRYAGGVDIPIG
                     THVSNRNRRELESLIGFFVNTLVLRIDLAGDPSFRTLVERVRRTTLDGLEHQDMPFDR
                     LVDRLGVERRPGVMPLFQVSFTLQQPPAPAPAESGLRFEAMVLDDEAARFDLDLQMWV
                     AGDALEGFWSYDRNLFDDGTARRMADHFLHLLGRLVREPDRPIAQLPILGSAERTLVE
                     RWNATDRDLPFVPLHDQVAAWARRTPEAPAIRHREQTWTYGQLDARSDRLARALRRRG
                     VGPETVVAIALRRCPEMALAVLAVGKSGGAFLPLDPDYPAERLRFMLEDSGARLLLTA
                     GELAAAPPVRDLEVLDLGRLADLDADGRASTADDPAVPDLRPDHLAYVMYTSGSTGRP
                     KGVACTHGGLSNIAQVGRDALQTGPGHRVLSFAPYSFDVSLWDLATAITAGATLCVAA
                     QEDLQPGAPLQTLLRAQRITHATLPPTALRHVSPDGLPDLEVMISGAEACPQELVDAW
                     AGRLRFGNVYGPTEAAIWATLKRFDAPEPVTIGRPLSNYRAYVVDARLEPVPIGVVGQ
                     LALGGLGLARGYHGQPSLTASRFVPSPFAGTPGERLYLTGDLARRNARGEIVCLGRID
                     NQVKLRGYRIELGEIEATLVDHEAVDDAVVVARTESGHPQLAAFLVASRPSADLVAAV
                     REHARRRLPAHMVPGLWKVLDRLPATPNGKIDRTALAAMRPDRGAVQREIVAPRNAVE
                     HRLAGAWARLLGVPQVGIHDNFFELGGDSILAVRLIAELQEAGLAIDAQQLIEHQTIA
                     ELAPLVAGAEAAIPARRLIVALRRGGSKPPLFTIPGGGGNPFYLHPLVRHLDGERPVL
                     ALRAPGLDGRESPSASVEDVAERYVAALRAELPQGPFLLAGHSFGAWVAFHVAWRLRQ
                     DGD"
BASE COUNT          382 a          949 c         1106 g          316 t
ORIGIN      
        1 gtcgccgtcc tggcgcagcc gccaggcgac gtggaacgcg acccaggcgc cgaacgagtg
       61 gccggcgagc aggaacgggc cctgcgggag ctcggcgcgc agcgcggcga cgtagcgctc
      121 ggccacgtcc tcgaccgagg cgctcggcga ctcgcggcca tcgaggccgg gcgcgcgcag
      181 cgcgaggacg gggcgctcgc cgtcgaggtg gcggaccagc gggtgcaggt agaacgggtt
      241 gccgcctccg cccgggatcg tgaacagagg cggcttcgag ccgccgcggc gcagcgccac
      301 gatcagccgg cgcgccggga tcgcagcctc ggcgccggcg accagcggcg ccagctcggc
      361 gatggtctgg tgctcgatca gctgctgcgc gtcgatcgcg aggccggcct cctgcagctc
      421 ggcgatcagg cggacggcga ggatcgagtc cccgccgagc tcgaagaagt tgtcatggat
      481 gccgacctga gggacgccga gcagccgcgc ccaggcgccg gcgaggcggt gctcgacggc
      541 gttgcgcggc gccacgatct cgcgctggac cgcgccgcga tccggacgca tcgccgccag
      601 ggccgtgcgg tcgatcttgc cgttgggcgt ggccggcagg cggtccagga ccttccacag
      661 cccgggcacc atgtgcgccg gcagccgccg gcgcgcatgc tcgcgcaccg ccgcgacgag
      721 gtcggcggac ggccggctcg cgacgaggaa ggccgcgagc tgcgggtggc cgctctcggt
      781 gcgggcgacg acgacggcgt cgtcgacggc ctcgtggtcg acgagggtgg cctcgatctc
      841 gccgagctcg atgcggtagc cgcgcagctt gacctggttg tcgatgcggc cgaggcagac
      901 gatctcgccg cgggcgttcc ggcgggcgag gtcgccggtg aggtagaggc gctcgccggg
      961 ggttccggca aacggcgaag gcacgaagcg cgacgccgtc agggacggct ggccatggta
     1021 gccgcgcgcc aggccgaggc cgcccagcgc gagctggccc acgacgccga tcggcaccgg
     1081 ctcgagccgc gcgtcgacga cgtaggcgcg atagttgctc agcggccggc cgatggtcac
     1141 cggctcgggc gcgtcgaacc gcttgagcgt cgcccagatc gcggcctcgg tggggccgta
     1201 gacattgccg aagcgcaggc gcccggccca ggcgtcgacg agctcctgcg gacaggcctc
     1261 ggcgcccgag atcatgacct cgagatcggg cagcccatcc ggcgagacgt ggcgcagcgc
     1321 cgtgggcggc agggtcgcgt gggtgatccg ctgcgcgcgc agcagcgtct ggagcggcgc
     1381 gccgggctgc agatcctcct gggcggcgac gcagagggtg gcaccggcgg tgatcgccgt
     1441 cgccagatcc cagagcgaga cgtcgaagct gtagggcgcg aacgacagca cgcggtggcc
     1501 cggcccggtc tggagggcgt cccggccgac ctgggcgatg ttgctgagcc cgccgtgcgt
     1561 gcacgccacg cccttcggcc gcccggtgga gcccgaggtg tacatgacgt aggccaggtg
     1621 atcgggccgc agatccggga cggccggatc gtccgcggtc gatgcgcggc cgtccgcgtc
     1681 caggtcggca agccgcccca ggtcgagcac ctcgaggtcg cgcaccggcg gcgccgccgc
     1741 gagctcgccg gcggtgagca gcaggcgagc gcccgagtcc tcgagcatga agcgcaggcg
     1801 ctcggcgggg tagtcggggt cgagcgggag gaacgcgccg ccggacttgc cgacggcgag
     1861 cacggcgagc gccatctcgg ggcaacgccg cagcgcgatc gcgaccacgg tctcggggcc
     1921 gacgccgcgg cggcgcagcg cgcgcgcgag gcgatcgctg cgggcgtcga gctggccgta
     1981 cgtccaggtc tgctcgcgat ggcggatcgc cggggcctcc ggcgtgcggc gcgcccacgc
     2041 agcgacctgg tcgtgcagcg ggacgaacgg aaggtcgcga tcggtcgcgt tccagcgctc
     2101 gaccagggtg cgctcggcgc tgccgaggat cggcagctgc gcgatcggcc ggtcgggctc
     2161 gcgcacgaga cgcccgagca ggtggaggaa gtgatcggcc atgcggcgcg cggtgccgtc
     2221 gtcgaacagg ttgcggtcgt agctccagaa gccctcgagc gcatcgccgg ccacccacat
     2281 ctggaggtcg aggtcgaacc gcgcggcctc gtcgtcgagg accatcgcct cgaagcgcag
     2341 cccgctctcg gccggcgccg gcgccggcgg ctgctggagg gtgaacgaca cctggaacag
     2401 gggcatgacg ccgggccggc gctcgacgcc gaggcgatcg accaggcggt cgaacggcat
     2461 gtcctggtgc tcgaggccgt cgagggtggt ccggcggacg cgctcgacca gggtgcggaa
     2521 gctgggatcg ccggcgaggt cgatgcgcag cacgagggtg ttgacgaaga acccgatcag
     2581 cgactcgagc tcgcgccggt tgcggttcga cacgtgcgtg ccgatcggga tgtcgacgcc
     2641 gccggcgtag cggccgagga cgaccgcgaa cgcggcgatc aggaccatga acagggtgga
     2701 gccggtcgcc tggcccagcg cctcgacgcg cgccgcgacg tcggcgccga cgc
//
LOCUS       DASVOR010000132        14961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_264204,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000132 DASVOR010000000
VERSION     DASVOR010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14961
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264204"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1747)
                     /locus_tag="VF516_02720"
     CDS             complement(<1..1747)
                     /locus_tag="VF516_02720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX8106608.1"
                     /translation="MNLFSLLARRVAEDPARLAYGFEADGGGEDSLTYGQLARRAGAI
                     AARLRETARPGDTALLLLPQGLDYIVAFWACVQANVIAVPLFPPRRNRGGDRVEAVIT
                     DSGARLALTDAATLSGLDRGVQESPYLRELRWVDVQAIDPEATSAPAVDVDVDRDPDT
                     IAFLQYTSGSTGTPRGVMVSHRNLLTNLRDIDAGIGHTPASVGISWLPFFHDMGLIYG
                     VLMPVYAGFPCYFLTPAAVMQRPGRWLETISRRRGTHSGGPNFIYDLCVRSIRPEQRA
                     GLDLRSWTMAHNGAEPVRHATLERFAAAFRPHGFDPAAFCPCYGLAEATLKVSAVPMW
                     DAPRTFFAREAGLQRHRVERAAGAGDGSVALVGCGASAIDTRILVVEPETRRPCEPGE
                     IGEIWVAGDTVARGYWRRPEETAATFQARLAGSEDGPFLRTGDLGTFIEGELVITGRC
                     KDLLILRGRNHYPHDIETTVGVCHDALEVDAAAAFSIEVDGEERLVVVQEVRRTALHA
                     LDADQVIAAISGAVTEHHDLQVHAVALLSPKRLPKTSSGKVQRQACRQAFLDGTLQAV
                     ATWTRHTPPAAGPEEP"
     gene            complement(1798..3450)
                     /locus_tag="VF516_02725"
     CDS             complement(1798..3450)
                     /locus_tag="VF516_02725"
                     /EC_number="5.4.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR04475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine aminomutase (D-beta-phenylalanine
                     forming)"
                     /protein_id="HEX8106609.1"
                     /translation="MIDSASAANITVGDSRPWVLRGDARMSLEELCDLSVPGHQIEVD
                     GDTLARIERCRAKLTEYVERGRVMYGVNTGVGGFVDRLVPVDHAAQLQNNIINAVATN
                     VGGYLDERATRAIMLARIKSLARANSAISVENFGKLVEMFNAGVAPCVPEKGSLGTSG
                     DLAQLASIALVGSGQWRARLDGCELPGAEALHRAGIEPMALSFKEGLALINGTSGMVG
                     LGALVLAEAKALLDTYIAVSALSCEGLAGKTKPFDPRVHALKPHRGQQRVAEMMWGRL
                     SDSKLALDEDSVEHILAARMRGDAAAGHGQMQIEDAYSIRCTPQILGPVLDSMAAIER
                     VLEDELNSSNDNPLLVPDQDDLFHNGHFHGQYIAMAMDHLAIALVTLCNLADRRIDRF
                     MTGANSNGLPPFLCENDAGLRLGLMGGQFMATSLTAENRSLCTPISIQTLPSTGDVQD
                     VVSFGFVAARRARTVLANTRYVVAFELLCACQAADIRGPHGLSASGRMLYEATRQIVP
                     YLDHDIVLTDYLEALARGLLDGRIARAIHTHGVAQQPALSLL"
     gene            complement(3550..6660)
                     /locus_tag="VF516_02730"
     CDS             complement(3550..6660)
                     /locus_tag="VF516_02730"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8106610.1"
                     /translation="MKIAEIAIRRPVFGVMAILVPIVFGLMAYPRIGIEQFPSVEFPI
                     VTVLAVYPGADPASMETKVTKPMEDALSSIGGIKKLQTFSLESTTEAVIEFQLDVDPD
                     KAVQNVRDRLAAIPNLPRELEPPKIQKFDFGAAPIMSLALSGGLPPRELTKLAEDVVK
                     QRLGQIKGVGDISIVGGRPREIHVVIEPARLAARGLTPADVTAALDGGNLELPAGRIL
                     EHGRDVVVTTKGAFTGLQEIADAPIAASAAGGASSAVRVGDVASVRDDMQEARSYASV
                     NGTSALGLVIQKESGANLVAVARAVRAEIAAMQHMLADRGAQLTITEDQAPFIEGSFR
                     DVQFDLLLGAILAIGIILGFLRDGRATFISALALPTSVVATVWFLSLLGFSFNQMTML
                     ALSLSIGLLIDDAIVVIENIHRHLEQGKTPLRAAAEATSEIGLAVLATTSSIVAVFVP
                     VATMHGIIGRFFFQFGITVSVAVLLSMLVSFTLTPMLSGRLLRPQRHRRGLASRAIER
                     GLVALERVYRRLLGAALRRRAVTTVLAGGVLLGSCALVTRVKTEFLPSDDRSSFSVGI
                     ELPTGTTLATTQDVADRLAADLRARGPGVISTFVTAGRGSASAHIAQIQVDLRPVRQR
                     AFSQQAAMSWVREHYAPLASGGIKITLAEVGAVGGGDPRPIQLNLRSRNMDELVKAAD
                     ALAAELRKTKGFVDVDTSYRGGKPEIEVVPDRAAAAELGVPVATIASTVRALVSRDKI
                     TSYKEDADLYDIRLTLTDPVQADFPALSNLMVRSNTGELVPLSSVVRLVRGTGPTQIS
                     REARMRQITVLAGLDGLALGEASARTIELARRVVPASVETEMAGTSQLMAESFGYMIV
                     ALILAVALVYMILAAQFESFIHPLTIMISLPLAVVGAFGGLFLAGQTLSIFGMIGLIM
                     LMGLVTKNAILLVDFAQKQKAQGASTRDALLVAGPIRLRPILMTTAAMILGMLPVALG
                     LGESGAGRSSMAVVVIGGLITSTMLTLVMVPVIFSLIEGLRERVRRRGPQAIPTATVV
                     DEPASP"
     gene            complement(6676..7635)
                     /locus_tag="VF516_02735"
     CDS             complement(6676..7635)
                     /locus_tag="VF516_02735"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8106611.1"
                     /translation="MTDSPELHLMQARRRSMGDFMLTQGSNALLAVAVMLAGCGKGSD
                     AGAALPPATGPGSPPLPALAGVSGPAETPSATIASTGRSTGTLLPHSEVAVVARSRGV
                     VVALTIDVGARIEQGKVLFRVDDREAGLRLAQAQTQLAAARQQASSAEVEYRRTQKLF
                     EQQAVSPQQWDQISAQHDAAKIGVAQAQNGVAIASKAVTDTTVRAPIGGVVVARRVAL
                     GDYVTDGAPVLVVQDQDRLDLRFRLPDRALLKVGVGDAITVSLPALGVSRKATISLVA
                     PSVDPRTRTIELTAVLDNRDGRLRPGLMAEVEFDAKLASASNH"
     gene            complement(7632..8960)
                     /locus_tag="VF516_02740"
     CDS             complement(7632..8960)
                     /locus_tag="VF516_02740"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8106612.1"
                     /translation="MLLVVTIATGSLRPAHAGDAAPPPGAARRLTMAEAIDAALAHNP
                     ELAVATETIAAATARTEADASLRWPLLNVRANALLWNRAIVADLGADIGKITIRDRVT
                     GSVDVSVTQPLTGALVTGKLVSRDRSATEASRAQRDGLRVDVAYRTAEAYLDALQAAT
                     LSKVAEATLLKLDADLQHAKTLLQAGTLQRVDVLRLEVERARVEQQRLQADTGALGAR
                     RRLALLLGYPDGSELELVEIDATPPLPWTEDEAVQRAHHDRADERVADANRRAADLGI
                     DISRARYFPAVSAVGVYSHAISTAVFGSAAESAYVGVSLDWNLWDWGGRSAELDGARA
                     LSRQAELTQHALADQIAVGARARWQAARTAQATLEVAARALTAAVEAQRLEAVRFAQG
                     AATTVEMIDTEAALATAQAQAAINRYQYLVAWMALSREVGALPAMPQDRR"
     gene            complement(9009..9230)
                     /locus_tag="VF516_02745"
     CDS             complement(9009..9230)
                     /locus_tag="VF516_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HEX8106613.1"
                     /translation="MRDDGNEDTTIYKVVLNHEEQYSIWPADRANPPGWRDAGKQGSK
                     AACLEFIDAVWTDMRPLSLRKAMEAAAAE"
     gene            9576..13001
                     /locus_tag="VF516_02750"
     CDS             9576..13001
                     /locus_tag="VF516_02750"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106614.1"
                     /translation="MDLSGRRLGEFTLREQIGEGGCAVVYRCEQPQLRRSAVVKVLHE
                     RGQRSDAALDRFRREAQLASQLDHPYAAHVYAFGDEDDGLFWIAMELVQGTALDDWLE
                     AHGPMPLAQFVPFFECVADVVHAAHERGIIHRDLKPSNVMVIDSNGRLFPKLLDFGIA
                     KLIGELALAVPEHPEGTEAVTTAPLRAAERRAQRTRTDPADQEHRLTRTSAKLGSTAY
                     MSPEQWTNPQAVGPASDIYSLGILAYEVLTGRKPFTAESTHEYYHRHVYAEAPPLGEG
                     FPPEDDRVIRRALAKAPEARHGNALELAAELRKALRTSRREQLRTSAQQWDDDRRPRG
                     LLWGADVLEETLRSVPQQTLSPLESMFLKDSQRWIRRARWVRRALAAIAAASVLAAVL
                     LQIRAARQVTEATITQAELEQGRSALLHEEPEAQLHLSRAYQRGDHSPSTAFMLARAL
                     QPRLAEQARLASIAGRMWSAAFSPDGRQIVTTDDRAAQVWDAQTYRRTSVLFHGDTVY
                     QALYSADGARIVTAGGDGTVKIWDAATGGLVRELRHDGARLRYFIAALSPDGRFVAAI
                     NTRGDLTHVWDTATGATVAEIRGEGLEFPGLAFSPDGRWLTATGGGDVCVIDARTWKE
                     VVAIRGPRVRSLAFDPTGARLVTGDATGEVAIWEIPSGARLRRLREAGESIYAVAYSP
                     DGRLVAAGSRDGVVQVWQGESGELQGQLNARHSKVLAVEFDRASKTVLAAGVDGAVVV
                     AEAGEATPVAVLDGPRSLMIAHFDPDARRVIGASLEGTAQIWDATPPYRRWRSPPVAD
                     DCGLAPGSTSGSTPDGRFVAIGCRDHPTRVWDTVHDQLLAELPNTTLVADDSPSASPA
                     VSSVGDRAAIARGNVVEVYEIPGGRLGRTIAHGAPVSALAFAPTGRDVVSGATDGSLL
                     VTRDGGARIALLASPGGVDAVGFLPDGRVVAADAQRRLRVFDPGGTVLADLEIPTRLA
                     LLRIDGARLVGVPYTESAAPSLLIDVERYRVVARLEGYTGQLFSARWTTDGQILTAGT
                     DGTARLWDGATGRLLQTYRGSSRFLADATLTADGLVVASGADGLLRFWERNSGCLLWA
                     LPAHISQVYGVHADGADIVTRGITGELARWRLSSPEQVIQACGDHERCAIVEP"
     gene            12998..13180
                     /locus_tag="VF516_02755"
     CDS             12998..13180
                     /locus_tag="VF516_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106615.1"
                     /translation="MKKTPRKLVLRNQTLRVLADLDLSRAAGGNGSGDAPCTGLAGTG
                     AAACPTTVAAVSAAAC"
     gene            13217..13603
                     /locus_tag="VF516_02760"
     CDS             13217..13603
                     /locus_tag="VF516_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106616.1"
                     /translation="MTSALASTAVSQRRWRLSTAALVLDVGVRRPLQTYRGSSRFLAD
                     VTRMDGGLVVAGGADGMLRFWDRDSGYLLWALPAHRSKIYGVHAEGDNLVIRGITGEL
                     TRWTLPAPHQVTEACGDHERCAILTR"
     gene            complement(13949..14350)
                     /locus_tag="VF516_02765"
     CDS             complement(13949..14350)
                     /locus_tag="VF516_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106617.1"
                     /translation="MEKVFSDKSGDADFFARVPEGVLWIDCVSPAPKDPQSDLPEFVA
                     DVIIDKWRKKFARTAASSALPSAVAVNLLKNQENLMPRLVFDEITKQQNYLAPARIWR
                     ECPSLQRAWIGLPSWSDRAQRPNVIASWSRP"
     gene            14570..>14961
                     /locus_tag="VF516_02770"
     CDS             14570..>14961
                     /locus_tag="VF516_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106618.1"
                     /translation="MKKTARKTGTTEATRPQLREVLGKLADDQATVQLVLGLQYLVRN
                     QLREFVIGAGTAALAAVLEEERTRLVGPKYAHLPDRQAHLPAFDQSAVFAPALPRDCL
                     WPRSQRATEGRLPAPSRSSWGRPSSRPQG"
BASE COUNT         2267 a         5217 c         5300 g         2177 t
ORIGIN      
        1 cgggctcctc gggaccggcg gccggcggcg tgtgccgcgt ccaggtggcg accgcctgga
       61 gcgtgccgtc gaggaacgcc tggcgacagg cctggcgctg gaccttgccg ctcgaggtct
      121 tcgggagccg cttcggggac aacagcgcca cggcgtgcac ctgcagatcg tggtgctcgg
      181 tgacggcgcc ggagatcgcg gcgatgacct ggtcggcgtc gagcgcgtgc agcgcggtgc
      241 gccggacctc ctgcaccacc acgaggcgct cctcgccgtc gacctcgatc gagaacgcgg
      301 ccgcggcgtc gacctcgagg gcatcgtggc agacgccgac ggtcgtctcg atgtcgtgcg
      361 ggtagtggtt cctgccgcgc aggatgagca ggtccttgca ccgcccggtg atcaccagct
      421 cgccctcgat gaaggtgccc agatcgccgg tgcgcaggaa cggaccgtcc tcgctcccgg
      481 cgagacgggc ctggaaggtc gccgcggtct cctccggtcg gcgccagtag cctcgtgcca
      541 cggtgtcgcc ggcgacccag atctcgccga tctcgcccgg ctcgcagggc ctgcgcgtct
      601 ccggctcgac cacgaggatc cgcgtgtcga tcgccgacgc cccgcagccg acgagcgcga
      661 cgctgccgtc gccggctccg gcggcacgct cgacgcggtg gcgctgcaag ccggcctcgc
      721 gcgcgaagaa cgtcctgggc gcgtcccaca tcgggaccgc ggagaccttc agcgtcgcct
      781 cggcgaggcc gtagcacggg cagaacgccg ccgggtcgaa gccgtggggg cggaacgccg
      841 cggcgaaccg ctcgagcgtc gcgtggcgca ccggctcggc gccgttgtgc gccatggtcc
      901 acgagcggag gtcgagcccg gcgcgctgct cgggccggat cgaccgcacg cacaggtcgt
      961 agatgaagtt ggggccgccg ctgtgggtgc cgcgccgccg ggagatcgtc tcgagccagc
     1021 gcccgggccg ctgcatgacc gcggcgggcg tcaggaagta acacgggaac ccggcgtaga
     1081 ccggcatcag cacgccgtag atgaggccca tgtcgtggaa gaacggcagc cacgagatgc
     1141 ccacgctcgc gggggtgtgg ccgatccccg cgtcgatgtc gcgcaggttg gtcagcaggt
     1201 tgcggtgcga gaccatgacg ccgcgcggag tgccggtcga acccgaggtg tactggagga
     1261 aggcaatggt gtcgggatcg cggtcgacat cgacatcgac ggcaggcgcg gaggtcgcct
     1321 ccggatcgat ggcctgcacg tcgacccagc ggagctcgcg gagatagggg gattcctgga
     1381 cgccgcggtc cagaccgctc agcgtggcgg cgtcggtgag cgcgaggcga gcgccggagt
     1441 cggtgatgac cgcctcgacc cggtcgccgc cgcggttgcg ccggggcggg aacagcggca
     1501 cggcgatgac gttcgcctgc acgcaggccc agaacgccac gatgtagtcc agcccctggg
     1561 gcaggagcag cagcgccgtg tcaccgggcc tggcggtctc gcgcagccgg gcggcgatcg
     1621 cgccggcgcg tcgggccagt tgcccgtagg tcagcgagtc ctcgccaccg ccgtcggcct
     1681 cgaagccgta ggcgagacgt gcaggatctt cagcgactcg tcgcgcgagc aaggagaaca
     1741 ggttcatggg atcccggtga cggaggatgg aaggtggccc gacgtgctcg cgcccggtca
     1801 cagcagcgac agggcgggct gctgcgcgac gccgtgcgtg tggatcgcgc gtgcgatgcg
     1861 accatcgagc agcccgcgcg cgagcgcctc gaggtagtcg gtcaggacga tatcgtgatc
     1921 gagatacggg acgatctgcc gcgtcgcctc gtacagcatc cggcccgacg cggagagccc
     1981 gtgtggcccc cggatatcgg ccgcctgaca ggcgcacagc agctcgaacg ccacgacgta
     2041 cctcgtgttg gcgagcaccg tgcgggcgcg ccgcgccgcg acgaacccga acgacacgac
     2101 gtcctggacg tcgccggtcg acggcagcgt ctggatcgag atcggcgtgc acagactgcg
     2161 gttctcggcg gtgagcgacg tcgccatgaa ctgtccgccc atcaggccga gccgcagccc
     2221 cgcgtcgttc tcgcacagaa acggcggcaa gccgttgctg ttcgcgccgg tcatgaagcg
     2281 gtcgatccgc cgatccgcca ggttgcacag cgtgaccagc gcgatcgcga gatgatccat
     2341 cgccatggcg atgtactggc catgaaagtg gccgttgtga aacaggtcgt cctggtccgg
     2401 aacgagcagc gggttgtcgt tggacgagtt cagctcgtcc tcgaggacgc gttcgatcgc
     2461 cgccatcgag tcgagcaccg ggccgaggat ctgcggcgtg cagcgaatcg agtacgcatc
     2521 ctcgatctgc atctgaccgt gtccggccgc tgcgtcaccg cgcatgcgcg cggccaggat
     2581 gtgctcgacg ctgtcctcgt cgagcgcgag cttcgagtcg ctcagccgcc cccacatcat
     2641 ctcggcgacc cgctgctggc cgcggtgcgg cttgagggcg tggacccggg ggtcgaacgg
     2701 cttggtcttg ccggccagcc cctcgcacga cagcgccgac acggcgatgt acgtgtcgag
     2761 gagcgccttg gcctcggcga gcacgagcgc gccgagcccc accatgccgg atgtaccgtt
     2821 gatcagcgcg aggccctcct tgaacgacag cgccatcggc tcgatcccgg cgcggtgcag
     2881 cgcctcggcg ccgggcagct cgcagccatc gagccgcgcc ctccactgcc cgctcccgac
     2941 cagcgcgatg ctggcgagct gggcgaggtc gccgctggtg ccgagcgagc ccttctccgg
     3001 cacgcacggc gcgactcccg cgttgaacat ctcgacgagc ttgccgaagt tctcgaccga
     3061 gatcgccgag ttcgcgcgcg ccagcgactt gatccgcgcg agcatgatcg cgcgcgtcgc
     3121 gcgctcatcg aggtagccgc cgacgttcgt ggccaccgcg ttgatgatgt tgttctgcag
     3181 ctgcgctgcg tggtcgaccg gaaccagccg gtcgacgaac ccgccgacac cggtgttcac
     3241 gccgtacatg acacggcccc gctcgacgta ctcggtcagc ttggcccggc agcgctcgat
     3301 gcgagcgagc gtgtcgccgt cgacctcgat ctggtgcccg ggtacggaca gatcgcagag
     3361 ctcctccagg gacatcctgg cgtcgccgcg caggacccag ggacgagagt cgccgacagt
     3421 gatattcgca gcagacgcag agtcgatcat ggtggcaccc ccgatgcact caagggaata
     3481 gagacagcgc ggcggttttg tgagcgcgcc acgtatattt ttcgccagcg aactggccgc
     3541 ggaccgggtt cacggcgacg ccggctcgtc gacgacggtg gcggtcggga tcgcctgggg
     3601 accgcggcga cgcacccgct cgcgcagccc ctcgatcagc gagaagatca ccgggaccat
     3661 gacgagggtg agcatcgtcg atgtgatcag gccgccgatc acgacgaccg ccatcgagga
     3721 gcgtcctgca ccgctctccc ccaggccgag cgccaccggc aacattccga ggatcatcgc
     3781 cgcggtggtc atgaggatcg gccgcaaccg gatcggcccg gccacgagca gcgcatcccg
     3841 ggtcgacgcg ccctgcgcct tctgcttctg cgcgaagtcg accagcaaga tcgcgttctt
     3901 ggtgaccagc cccatcaaca tgatgaggcc gatcatgccg aagatgctca gcgtctgacc
     3961 ggccaggaac aggccgccga acgcgccgac caccgccagg ggcagcgaga tcatgatcgt
     4021 gagcgggtgg atgaagctct cgaactgcgc cgcgaggatc atgtagacca gcgcgaccgc
     4081 caggatcagc gcgacgatca tgtagccgaa cgactcggcc atcagctggc tggtaccggc
     4141 catctcggtc tcgaccgatg caggcacgac gcgcctggca agctcgatgg tcctggccga
     4201 ggcctcgccg agcgcgagac cgtcgaggcc ggcgaggacc gtgatctgcc gcatgcgggc
     4261 ctcgcgcgag atctgcgtcg ggcccgtgcc gcgcacgagg cgtacgacgg acgacagcgg
     4321 caccagctcg ccggtgttcg accgcaccat gaggttcgac agcgcaggga agtcggcctg
     4381 caccgggtcg gtcagggtca ggcgaatgtc gtagaggtcg gcgtcttcct tgtaactggt
     4441 gatcttgtcc cgcgatacca gggcgcgcac cgtgctggcg atggtggcga ccggcactcc
     4501 gagctcggcc gcggcggcgc gatcgggcac gacctcgatc tccggcttgc cgccgcgata
     4561 gctcgtgtcg acatcgacga accccttggt cttgcggagc tccgcggcca gcgcgtccgc
     4621 ggccttgacc agctcgtcca tgttccggct gcgcaggttg agctggatcg gtcgaggatc
     4681 gccgccgccg accgcgccga cctcggcgag cgtgatcttg atgccgccgc tggccagcgg
     4741 cgcgtagtgc tcgcgcaccc agctcatcgc cgcctgctgg ctgaacgcgc gctggcgcac
     4801 gggccggagg tcgacctgga tctgcgcgat gtgggccgac gccgagccgc ggccggcggt
     4861 gacgaacgtc gagatcacgc ccgggccgcg ggcgcgcagg tcggcggcca gacggtctgc
     4921 gacgtcctgc gtcgtggcca gcgtggtgcc ggtcggcagc tcgatgccga ccgagaacga
     4981 cgagcgatca tcgctgggaa ggaactcggt cttgacccgg gtgaccagcg cgcacgatcc
     5041 gaggagcacg ccgccggcga gcaccgtggt caccgcgcga cggcgcagcg ccgcgccgag
     5101 cagccgccgg tacacccgct cgagcgccac caggccgcgc tcgatcgcgc gggacgccag
     5161 gccccgacga tggcgctgcg gccggagcag ccgccccgag agcatcggcg tcaaggtgaa
     5221 cgacaccagc atcgacagca gcaccgcgac gctgaccgtg atgccgaact ggaagaagaa
     5281 ccggccgatg atcccgtgca tcgtcgcgac cggcacgaac acggcgacga tcgaggacgt
     5341 cgtggcgagc acggccaggc cgatctcgga cgtggcctcg gcggcggccc gcagcggcgt
     5401 cttgccctgc tcgaggtggc gatggatgtt ctcgatcacc acgatggcat cgtcgatcaa
     5461 gaggccgatc gacaggctga gcgcgagcat cgtcatctgg ttgaacgaga agcccaggag
     5521 cgagaggaac cacacggtgg cgaccaccga cgtcggcagc gcgagcgcgc tgatgaacgt
     5581 ggcgcgaccg tcgcgcagga acccgaggat gatcccgatc gccaggatcg cgccgagcag
     5641 cagatcgaac tgcacgtcgc ggaacgatcc ctcgatgaac ggcgcctgat cctcggtgat
     5701 ggtcagctgg gccccccggt cggcgagcat gtgctgcatc gccgcgatct cggcgcgcac
     5761 ggcccgggcg acggcgacca ggttggcgcc ggactccttc tggatcacca ggccgagcgc
     5821 gctcgtgccg ttgaccgacg cgtacgatcg ggcctcctgc atgtcgtccc gcaccgacgc
     5881 gacgtcgccg acccggaccg cgctggacgc accgcccgcg gccgacgcgg cgatcggcgc
     5941 atcggcgatc tcctgcaggc cggtgaacgc acccttggtc gtcaccacca cgtcgcggcc
     6001 gtgctcgagg atccgcccgg ccggcagctc gaggttgccg ccgtcgagcg ccgcggtgac
     6061 atccgccggc gtcaggccgc gcgcggccag ccgcgccggt tcgatcacca cgtggatctc
     6121 gcgcggtcgg ccgccgacga tgctgatgtc gccgacgccc ttgatctgcc cgagccgctg
     6181 cttgacgacg tcctcggcca gcttggtgag ctcgcgcggc ggcaacccgc cggacagcgc
     6241 cagcgacatg atcggcgccg cgccgaagtc gaacttctgg atcttggggg gctcgagctc
     6301 cctggggagg ttcgggatcg ccgccaggcg gtcgcgcacg ttctgcaccg ccttgtcggg
     6361 gtcgacgtcg agctggaact cgatcacggc ctcggtcgtc gattccaggc tgaacgtctg
     6421 cagcttcttg atcccgccga tcgagctcag cgcgtcctcc atcggcttgg tgaccttggt
     6481 ctccatcgac gccggatcgg cgccggggta gacggcgagc acggtcacga tcggaaactc
     6541 gacgctgggg aactgctcga tcccgatgcg tgggtaggcc atgagcccga acacgatcgg
     6601 caccaggatg gccatgacgc cgaataccgg gcgccggatg gcgatttcgg cgatcttcat
     6661 gatgcgtcct cgggctcagt ggttggaggc actcgcgagc ttcgcgtcaa actcgacctc
     6721 ggccatcagg ccgggacgga gccggccgtc gcggttgtcg agcacggcgg tcagctcgat
     6781 ggtgcgcgtc cgtggatcga cgctgggtgc cacgagggag atcgtcgcct tgcgggagac
     6841 gccgagcgcc ggcagcgaga ccgtgatcgc gtcgccgacc cccaccttca gcagcgcgcg
     6901 atcgggcaac cggaaccgga gatcgagccg gtcctggtcc tgcaccacca ggaccggcgc
     6961 cccgtcggtg acgtagtcgc cgagcgccac gcggcgcgcg accacgacgc ccccgatcgg
     7021 cgcgcgcacc gtcgtgtcgg tcaccgcctt gctggcgatg gccacgccat tctgtgcctg
     7081 cgcgaccccg atcttggcgg cgtcgtgctg ggcggagatc tgatcccact gctgcgggga
     7141 gaccgcctgc tgctcgaaca gcttctgggt gcggcggtac tcgacctccg cgctgctcgc
     7201 ctgctggcgt gccgcggcga gctgggtctg cgcctgcgcg agccgcagcc cggcctcgcg
     7261 gtcgtcgacc cggaacagga ccttgccctg ctcgatgcgg gcgccgacgt cgatggtcag
     7321 cgcgaccacc acgccgcgcg atcgcgccac caccgcgacc tcggagtgcg gcagcaaggt
     7381 ccccgtcgat ctgcccgtcg aggcgatcgt cgcgctcggc gtctccgcag ggccgctgac
     7441 tccggccaga gccggcagcg gtggcgagcc tgggccggtc gcgggcggca aggccgcccc
     7501 ggcgtcgctg cccttgccgc agcctgcgag catgacagcc actgcgagca atgcattcga
     7561 tccctgcgtc agcatgaagt ctcccatcga gcgccggcgt gcctgcatga ggtgaagctc
     7621 cggtgaatcg gtcatcggcg atcctgcggc atcgcgggca gggctccgac ctcgcggctc
     7681 agcgccatcc aggccacgag gtactggtaa cgattgatcg ccgcctgcgc ctgtgcggtc
     7741 gcgagggcgg cctcggtgtc gatcatctcg accgtggtgg cggcaccctg agcgaaccgc
     7801 acggcctcga gccgctgcgc ttcgacggcc gcggtcagcg cccgcgccgc gacctccagc
     7861 gtggcctgcg cggtgcgcgc ggcctgccag cgggcccggg cgccgacggc gatctggtcc
     7921 gccagggcgt gctgggtcag ctcggcctgg cggctgagcg ctcgcgcgcc gtcgagctcg
     7981 gcagagcgcc cgccccagtc ccacaggttc cagtccagcg acactccgac gtacgcgctc
     8041 tcggcggcgc tgccgaacac cgcggtgctg atcgcatggc tgtacacgcc gaccgcggag
     8101 accgcaggga agtacctggc gcggctgatg tcgatcccca gatcggccgc gcggcggttg
     8161 gcgtcggcga cgcgctcgtc ggcgcggtcg tggtgggcgc gttgcacggc ctcgtcctcg
     8221 gtccacggca gcggcggcgt cgcgtcgatc tcgaccagct cgagctcgct gccgtccggg
     8281 tatccgagca gcagcgccag cctgcggcgc gccccgagcg ccccggtgtc cgcttgcaag
     8341 cgctgctgct cgacccgcgc gcgctcgacc tcgagccgca gcacgtcgac ccgctgcagc
     8401 gtcccggctt gcagcaaggt cttcgcgtgc tgcaggtcgg cgtcgagctt gagcaaggtc
     8461 gcctccgcca ccttgctcag ggtcgcggcc tgcagcgcat cgaggtaggc ctcggcggtc
     8521 cggtacgcga catcgacacg aagcccgtcg cgctgcgccc ggctcgcctc ggtggcgctg
     8581 cgatcgcggc tcaccagctt gccggtgacc agcgcgccgg tcagtggttg cgtgaccgag
     8641 acgtcgaccg agccggtcac ccgatcgcgg atggtgatct tgccgatatc cgcgccgaga
     8701 tccgccacga tcgctcggtt ccacagcagc gcgttggccc ggacgttgag cagcggccag
     8761 cgcagcgacg catccgcctc ggtccgcgcg gtcgctgcgg cgatggtctc ggtcgcgacc
     8821 gcgagctcgg ggttgtgggc gagcgccgcg tcgatcgcct cggccatcgt gagcctccgc
     8881 gcggcgcccg gtggtggggc cgcatcgccg gcatgcgccg ggcgcagcga gcccgtggcg
     8941 atggtgacca ccagcagcac ggatcgaaca ctgtgcttca tgagcttctc cgctgcgtcg
     9001 cgcacgagct actctgccgc cgcagcctcc atcgccttgc gcaggctcag cggccgcatg
     9061 tcggtccaca ccgcgtcgat gaactccagg caagctgcct tgctgccctg cttgccggcg
     9121 tcgcgccagc ccggcgggtt cgcgcgatcg gcaggccaga tcgagtattg ctcctcgtga
     9181 ttgaggacga ccttgtagat ggtcgtgtct tcattgccgt catcgcgcac gatcgacccc
     9241 ccggtgcgcc cagatggagc gcgcaaggat tggagacgcg ccggttgttt tgtgagcggc
     9301 aaggcgaaaa tgaagcggtg agctgagctc gaagcgaggg cgcgccggcc ctaagtgatg
     9361 aaaactcggg ccgtcctggc cacaggcgcc tggccgactg gcggcggcct acaccagcag
     9421 acagatcgag gaacggacgc tggtaaggaa acgtgagggt gcgagctgca gtcgcgagct
     9481 gcacctgacg cggatgctgc aaaatgcgac cgtagcacct actcccagta gatggccgac
     9541 gcttagtcta tccccccgcg gaggagtcac ggccgatgga tctttcagga cgcaggctgg
     9601 gcgagttcac cctgcgcgag cagatcgggg agggcggctg cgcggtggtg taccgctgcg
     9661 agcagccaca gctccggcgc agcgcggtcg tcaaggtgtt gcacgagcgc gggcagcgca
     9721 gcgacgctgc gctcgatcgg tttcggcgcg aggcgcagct cgcatcgcag ctcgatcatc
     9781 cctacgcggc gcacgtctac gcattcggtg acgaagacga cggcctgttc tggatcgcga
     9841 tggagctggt gcagggcacc gcccttgatg actggctgga ggcgcacggg ccgatgccgc
     9901 tcgcgcagtt cgtgccgttc ttcgagtgcg tcgccgacgt cgtgcacgct gcccacgagc
     9961 gcgggatcat ccaccgcgac ctgaagccgt cgaacgtgat ggtgatcgac agcaacgggc
    10021 gcctgttccc caagctgctc gacttcggga tcgccaagct gatcggcgag ctcgcgctcg
    10081 ccgtgccgga acaccccgag ggcaccgagg cggtgacgac cgcgccgctg cgagccgccg
    10141 agcggcgcgc ccagcgcacg cgcacggacc ccgcggacca ggaacaccgc ctgacccgca
    10201 cgagcgccaa gctcggctcg acggcgtaca tgtccccgga gcagtggacg aaccctcagg
    10261 ccgtcggccc ggccagcgac atctactcgc tcggcatcct cgcctacgag gtgctgactg
    10321 gacgcaagcc gttcaccgcc gagagcacgc acgagtatta ccaccggcac gtctatgccg
    10381 aggcaccgcc gctcggcgag ggctttccac ccgaggacga tcgcgtcatc cgtcgcgcgc
    10441 tcgccaaggc tcccgaagcc cgccacggca acgcgctgga gctggccgcg gagctgcgga
    10501 aggcgctgcg cacgagccgg cgcgagcagc tccggacctc ggcgcagcag tgggacgatg
    10561 accgccgccc gaggggcctg ttgtggggag ccgatgtgct ggaggaaacc ctgcgcagcg
    10621 ttccccagca gacgctcagc ccgctggaga gcatgttcct caaggacagc cagcgctgga
    10681 tccggcgcgc ccgctgggtc cggcgagcgc tcgctgcgat cgccgcggca agcgtactgg
    10741 ccgccgtgct cctgcagatc cgcgccgccc ggcaggtcac cgaggcgacg atcacgcagg
    10801 ctgagctcga gcagggccgc tcggcgctgc tccacgagga gcccgaggcg cagctccacc
    10861 tcagccgcgc ctaccagcgc ggagaccact cgccgagcac ggcgttcatg ctcgcacgag
    10921 cgctgcagcc gcgcctcgcg gagcaggcgc gcctcgcgag catcgccggc cgcatgtggt
    10981 cggccgcgtt ctcgcccgac ggccggcaga tcgtcacgac cgacgaccgg gccgcgcagg
    11041 tctgggacgc acagacctac cggcggacat ccgtgttgtt ccacggcgac accgtctacc
    11101 aggcgctgta cagcgccgac ggcgcgcgga tcgtgaccgc cggcggcgac ggcaccgtca
    11161 agatctggga cgcggcgacc ggggggctcg tgcgcgagtt gcggcacgac ggtgcgcggc
    11221 tgcgctactt cattgccgcg ctatcccccg atggccggtt cgtggccgcg atcaacaccc
    11281 gtggcgacct cacgcacgtc tgggacacgg cgaccggggc gacggtcgcc gagatccgtg
    11341 gcgaaggtct ggagttcccg ggtctcgcgt tcagccccga cggtcgctgg ctcaccgcga
    11401 ccgggggcgg cgacgtatgc gtcatcgacg cgcggacgtg gaaggaggtc gtcgcgatcc
    11461 gcgggccgcg cgtccgcagc ctcgccttcg acccgaccgg cgcgcgcctc gtcacgggag
    11521 acgcaaccgg tgaggtggcg atctgggaga tcccgagcgg cgcgcggctc cgccgtctgc
    11581 gcgaggccgg cgagtcgatc tacgcggtgg cgtactcgcc cgacggccgg cttgtcgccg
    11641 cggggagccg ggacggagta gtgcaggtct ggcagggcga gtcgggagaa ttgcagggcc
    11701 agctcaacgc gcgccatagc aaggtcctcg cggtcgagtt cgaccgagcc tccaagactg
    11761 tgctcgctgc cggcgtcgat ggggccgtcg tggtcgcgga ggccggcgag gcgacgccgg
    11821 tcgccgtgct cgacgggccg cggagcctca tgatcgcgca cttcgacccg gacgcacgcc
    11881 gcgtcatcgg ggcgtccctg gagggcacgg ctcagatctg ggacgcgacc ccgccgtacc
    11941 gtcgctggcg ctcgccgccg gtcgccgacg actgtggtct cgcgccgggc tccacgtcgg
    12001 gctccacgcc agatggccgt ttcgtcgcca tcggctgccg agatcacccg acgcgcgtat
    12061 gggacaccgt gcacgaccag ttgctcgccg agctgcccaa tacgacgctg gtcgccgacg
    12121 actcgccctc tgcgtccccc gcggtctcca gcgtagggga ccgcgcggcg atcgcccgcg
    12181 gcaacgtcgt ggaggtctac gagatcccgg gcgggcggct cgggcgcacg atcgcgcacg
    12241 gcgcgccggt cagcgcgctg gccttcgcgc caacggggcg ggatgtcgtc agcggcgcga
    12301 ccgatggctc gctgctcgtc acgcgcgacg gcggcgcgcg gatcgcgctc ctggcctcac
    12361 cgggcggcgt cgacgccgtg gggttcctgc ccgacggccg ggtcgtcgcg gcggacgcgc
    12421 agcggcggct gcgagtcttc gaccccggcg gcacagtcct tgccgacctc gagatcccga
    12481 cgcgcctcgc gttactgcgg atcgacggcg cccgcctcgt cggcgtcccg tacaccgaga
    12541 gcgcggcgcc gtctctgctg atcgacgtcg agcgctaccg cgtcgtcgct cggctcgaag
    12601 ggtacaccgg acaactgttc tcggcgcgct ggacgacgga cggccagatc ctcaccgcgg
    12661 gcaccgacgg caccgcccgg ctgtgggacg gggcgacggg tcggctcctc cagacctacc
    12721 gcgggagctc gcgattcctc gcggacgcga cgctcacggc cgatgggctg gtggtggcca
    12781 gtggtgccga tgggctcttg cggttctggg agaggaatag cggctgtctg ctgtgggcac
    12841 tccccgcgca catctcgcag gtctacgggg tacacgccga cggcgccgac atcgtgactc
    12901 gcgggatcac aggagagctc gcgcggtgga ggctatccag ccccgagcag gtgatccagg
    12961 catgcggcga ccacgagcgc tgcgctatcg tagaaccatg aaaaagacac cccgcaagct
    13021 cgtgctgcgc aaccagaccc tccgcgtgct ggccgacctc gacctttcgc gcgccgccgg
    13081 aggaaacggt tcgggagacg cgccgtgcac gggcctcgct ggcacgggag ccgcagcctg
    13141 cccgaccacg gtcgccgccg tctctgccgc ggcctgttag cgcagggcgc cgagggcggc
    13201 agcgacctcg acgccgatga cctcggcgct ggcgagcacg gccgtgtccc agcggcggtg
    13261 gcggctctcg accgcggcgc tcgtgctgga cgtcggggtg cgccgacccc tccagaccta
    13321 ccgcgggagc tcgcgattcc tcgcggacgt gacgcgcatg gacggcgggc tggtggtggc
    13381 cggcggagcc gacgggatgc tgcggttctg ggaccgggat agtggatacc tgctctgggc
    13441 gctccctgcc cacaggtcga agatctacgg agtccacgcc gagggcgaca acctcgtgat
    13501 ccgcgggatc acgggggaac tcacgcgctg gacgctgcct gcccctcacc aggtgaccga
    13561 ggcgtgcggc gaccacgagc gctgcgctat cctgacgcga tgaagaagac gcctcggaag
    13621 ctcgtactac gccaggagac tctccgagcg cttgccgccg tggagctcgc gcatcaggcc
    13681 gccggcgtgg tcaccagcgg cctgatgcgc gagctcggcg gtcgcctcgg cggcccggcg
    13741 gcggccagcc gctcgatcgc atgcgatgct tggggttccg ggttccagga ccaacggcga
    13801 gggagtttac ggtaggcggc cggacctccg agccgatgcc acgaagcagc aggaggggcc
    13861 acttcaggga ggcgatgggc ggctcatcac ccactaaacc aggtggagag catgaaaggg
    13921 ctcgcgtagc cacacaccct tgcgggttct agggtcggga ccaactggcg atcacgttgg
    13981 gacgctgcgc acgatcgctc cacgatggga gaccgatcca cgctcgctga aggcttggac
    14041 actcgcgcca aatcctggct ggtgcgagat agttctgttg cttcgtgatc tcgtcgaata
    14101 cgagtcgtgg catcagattt tcctgattct tgagcaggtt caccgcaacg gccgatggca
    14161 gcgctgagct agcagccgtg cgcgcaaact tcttgcgcca tttgtcgatg atgacgtcgg
    14221 caacgaactc aggcaggtcg gattgtggat cctttggcgc gggcgaaacg cagtcgatcc
    14281 agagcacacc ctccggcacc ctcgcgaaga aatcggcatc gccgctcttg tccgaaaaga
    14341 ccttttccac ttctaggtcc tcgcaccaat ccttcaacca gagcccccag caacactctg
    14401 cgagggtgtc gagcatctcg gtgcttggag ctgcgtctct gggctttgtc ggcgcctgca
    14461 gcttcttgcg caggaatgcc cggcgtagtc ccgatccctg ttgaaattga ggtggcggtc
    14521 caaccgaccc cgagaggatt ctcgaggcgc ttaccggaag gaaccgccga tgaagaagac
    14581 tgccaggaag acagggacaa cggaagccac ccgtcctcag ctccgcgagg tgctggggaa
    14641 gctcgccgac gaccaggcca ccgtgcagct cgtgctgggg ctgcagtacc tggtccgcaa
    14701 ccaactccgc gagttcgtga tcggggcggg gaccgcggcg ctggctgcgg tgctcgaaga
    14761 agagcgtacg cgattggtcg gaccgaagta cgcgcaccta ccggatcggc aagcccacct
    14821 accggcgttc gatcaatcgg cggtgttcgc accagcgctg ccgcgcgact gcttgtggcc
    14881 caggtcgcag cgcgcgactg agggccgcct gcccgccccc tcgcgcagct cgtggggtcg
    14941 gccgagctcg aggccccagg g
//
LOCUS       DASVOR010000133         3625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439560,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000133 DASVOR010000000
VERSION     DASVOR010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3625
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439560"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF516_02775"
     CDS             <1..475
                     /locus_tag="VF516_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106619.1"
                     /translation="DQDTTRPKVAHTWDGLWQWEQDGVQMPYVSIYDYLLSKANPYAG
                     KLNDTYRALELRMRQNNCQACHAPDNQGNSAQLEFFVYPNQALAGRHDIITQLTRNEM
                     PPKNNTLNLTAGIADPAERELLISLARDFESAGDQALGWEGDNKTEFQLPDPSAQ"
     gene            1110..2366
                     /locus_tag="VF516_02780"
     CDS             1110..2366
                     /locus_tag="VF516_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX8106620.1"
                     /translation="MKVVRAGTRVGAGACGTTSGSGNISSRFDVTPLTSDQPGEAPNI
                     DPQLKDVWGVTSLQDQFWAVAQATGQVIGLQPGGEPTVHIPLARESVRPGAPGGAPDT
                     TGTGIAVVSESDQILMDIGGNCAAASMIVGAQSGKIWAVNPNISQSTAFVLADRSNVG
                     AQFTGVAVIRLPAATTNPPTATATGGGSCGGGGTQPPLPTLQTQVLAVDFHNERVDIF
                     DVVDGALRRITPTGRQFVIPNLPTNFAPFGILATEDRVVLTAAQRQGPTTGEPNIDMQ
                     TPGDGLGVVAAFDLTGNLLWRTESNLFNIPWGMAMGDLRLCATSALLVAQHGNPTEID
                     GPNNQFGGAIIAMDIRTGKVIQPLLASDMQPVRVQGLWGLTFTTGLTTLNETRLHMGA
                     GPVTPENRGMREAEHGLFARLDTTTR"
     gene            2493..>3625
                     /gene="mutY"
                     /locus_tag="VF516_02785"
     CDS             2493..>3625
                     /gene="mutY"
                     /locus_tag="VF516_02785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEX8106621.1"
                     /translation="MRRTQAIAAAVVAHYAQVRRDLPWRRTRDPYAIWVSEIMLQQTR
                     VATVIPYWQRWMAKFPTVSALAEAPLDDVLAAWAGLGYYSRARNLHAGAQAVGDRFGG
                     ALPSCAAELREVPGIGPYTAGAIASIAFGERAPLVDGNVARVLARVFAIEHDIKSSAG
                     GKALWAAAGELMTTLPEAAAPGDLNQGLMELGATICAPTQPRCLACPLARDCAAARTG
                     RQDELPVVAARKKASELPVLERTVVWLEAAGEIVLVRRTPDGLFGGLWELPPLDVAVG
                     LGVTPDARPVAFHDQTLTHRRLRIAVVRGAMPAALVAPGDPGYDAIRRVTITGAAALG
                     IAASTTAILRTYEDTPWSSIPRHSPSSPKATPRSSRASRGSATT"
BASE COUNT          563 a         1284 c         1280 g          498 t
ORIGIN      
        1 cgatcaggac accacgcggc cgaaggtggc ccacacctgg gacggcctgt ggcagtggga
       61 gcaggacggc gtgcagatgc cgtacgtctc gatctacgat tacctgctgt cgaaggccaa
      121 cccctacgcc ggcaagctca atgacaccta ccgcgcgctc gagctccgga tgcggcagaa
      181 caactgccag gcctgtcacg cccccgacaa ccagggcaac tcggcgcagc tcgagttctt
      241 cgtctatccc aaccaggcgc tcgccggccg ccacgacatc atcacccagc tgaccaggaa
      301 cgagatgccg cccaagaaca acacgctcaa cctgaccgcc ggcatcgccg acccggccga
      361 gcgcgagctg ctgatcagcc tggcgcgcga cttcgagtcc gccggcgacc aggcgctggg
      421 ctgggaaggc gacaacaaga ccgagttcca gctccccgac ccctcggcgc agtagcggat
      481 cggagggatc ggccgggcgc cccccggaca cggcgaggac gggctggatg cgcacgcgct
      541 ggcgggcagc gctcgaaaca cggccggcca ggcgcgtcga gtggtgcgca gcgacgcgcg
      601 gaccgaacag atggagcgag gcgcgcccgc accacttcgc ccgcgcggac gtcaccgacg
      661 aggccccggt gagcgcccgc atccgcgcgg tgccgcccag ggattggccc cgaggtcatc
      721 ggggtgacag gtgcggtgcg gttgcatcgc acctgtcgtt atttgtgcgt tcccgatcga
      781 cccgccggcg cggagatcgt gcgaggtagt tgcgcccggt ctcgggagat ccgcacagcg
      841 ccgatccgca cgccagcgcg agctcgcacg ctggcggccg ctcgccctca tgtggcccat
      901 tcgcgccgtc gagtcgtgtc tcatatgcga tatacacata tgtgaagcac tcgtgtcgaa
      961 cactgctgca aggcatggct gctgtccgac gagggctcgt cgagcgccat cgacacgcgt
     1021 ttcaccgggc aggtatccag aaattggcgc tcatcggatg gcgcacgacc tgcagccccg
     1081 gtcagcgcgg agattccgca ggaggatgta tgaaggtagt cagggccgga actcgcgtag
     1141 gcgcgggcgc atgtgggacg acaagtggca gtggcaacat cagcagtcgc tttgacgtca
     1201 cgccgctgac gtctgatcag ccgggcgagg cgccgaacat cgatccgcag ctgaaggacg
     1261 tctggggggt cacctcgctc caggaccagt tctgggcggt cgcccaggcg accggccagg
     1321 tcatcgggtt acagcccggt ggcgaaccga ccgtccatat cccgctcgcc cgggagagcg
     1381 tgcgtcccgg cgctcccggc ggcgccccgg atacgaccgg caccgggatc gcggtcgtca
     1441 gtgagtcgga ccagatcctc atggacatcg gcggcaactg cgccgcggcc tcgatgatcg
     1501 tcggtgccca gtcgggaaag atctgggccg tgaaccccaa catcagccag agcacggcct
     1561 tcgtgctggc cgaccgctcg aacgtcggcg cgcagttcac cggcgtggcg gtcatccgcc
     1621 tgccggctgc gaccaccaac ccgccgactg cgaccgccac gggtggcggc agctgcggcg
     1681 gcggtggcac ccagcctccg ctgcccaccc tccagaccca ggtgctggcg gtcgacttcc
     1741 acaacgagcg cgtcgacatc ttcgatgtgg tcgacggcgc cctccggcgc atcacgccca
     1801 cgggccggca gttcgtgatc cccaacctgc cgacgaactt cgcgccgttc ggcatcctgg
     1861 cgaccgagga ccgcgtcgtc ctgacggccg cgcagcggca gggtcccacg accggcgagc
     1921 cgaatatcga catgcagacc ccgggtgatg gcctgggggt cgtggccgcc ttcgatctga
     1981 ccggcaatct gctgtggcgc accgagtcca acctgttcaa catcccgtgg ggcatggcga
     2041 tgggcgacct ccgcctgtgc gccacgagcg cgctgctggt ggcgcagcac ggcaacccca
     2101 ccgagatcga cgggccgaac aaccagttcg gcggcgccat catcgcgatg gacatccgca
     2161 cgggtaaggt gatccagccg ctgctcgcca gcgacatgca gcccgtccgg gtccaggggc
     2221 tgtggggcct gaccttcacc accggcctca cgaccctcaa cgagacccgg ctgcacatgg
     2281 gcgccgggcc ggtcacgccg gagaaccgcg ggatgaggga ggccgagcac ggtctgttcg
     2341 ctcgcctcga cacgaccacg cggtgatcgc ccgcgcctgc gcggcgcgac gcgatcgacg
     2401 ctgatcggac cgccgcagct cgggaccacc gagcgcggcg gcggcgtttc gggtggggtt
     2461 gcggccgcgc cgcgatcggg atacgaggag gcgtgcgacg cacccaggcg atcgcggctg
     2521 ccgtggtggc ccactacgcc caggtgcggc gcgacctgcc gtggcggcgg acccgcgatc
     2581 cgtatgcgat ctgggtcagc gagatcatgc tgcagcagac ccgggtcgcc acggtgatcc
     2641 cgtactggca gcgctggatg gcgaaattcc cgaccgtctc ggcgctggcc gaggcgccgc
     2701 tcgacgacgt gctcgcggcg tgggcgggcc tcggctacta ctcgcgcgcc cgcaacctgc
     2761 acgctggggc gcaggcggtc ggcgaccggt tcgggggggc cctgccgtcg tgcgcggcgg
     2821 agctgcgcga ggtgcccggg atcgggccct acaccgcggg cgcgatcgcg tcgatcgcgt
     2881 tcggcgagcg ggcgccgctg gtcgacggca acgtcgcgcg cgtgctggca cgggtgttcg
     2941 cgatcgagca cgacatcaag tcgtccgccg gcggcaaggc gctgtgggcg gcggccggcg
     3001 agctgatgac cacgctgccc gaggccgcgg cgccgggcga cctcaaccag gggctgatgg
     3061 agctcggcgc gacgatctgc gcgccgaccc agccgcgttg cctggcatgc ccgctcgcgc
     3121 gcgactgcgc ggccgcgcgg accggccggc aggacgagct gccggtggtc gccgcgcgca
     3181 agaaggcctc cgagctgccg gtgctggagc gcaccgtggt gtggctcgaa gcggccggcg
     3241 agatcgtgct ggtgcggcgc acgccggacg ggctgttcgg cgggctgtgg gagctgccgc
     3301 cgttagatgt tgcggtgggg ctcggcgtta cgccggacgc caggccggtc gcgtttcacg
     3361 accagacgct gactcaccgg cggctgcgaa tcgccgtcgt tcgcggcgcg atgcccgcgg
     3421 cgcttgtcgc gccgggcgat ccgggctacg acgcgatccg gcgggtgacc atcaccggcg
     3481 cagctgcgct cgggatcgcc gcctcgacca ccgcgatcct gcgcacctac gaggacaccc
     3541 catggagctc gatcccgagg cactcgccct cttcaccgaa ggctacacca agatcctcga
     3601 gggcatctcg cggctcggct acgac
//
LOCUS       DASVOR010000134         4380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_293597,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000134 DASVOR010000000
VERSION     DASVOR010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293597"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VF516_02790"
     CDS             <1..287
                     /locus_tag="VF516_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106622.1"
                     /translation="LRALDDRDVLADRQRHRHRSTSRAAGSASVRRSVPSSCRRHEKV
                     ITRAGAGVRGPAPPGLLRRGLAYSPNRIASHSPISSTPAMAPHDGDARHG"
     gene            complement(369..665)
                     /locus_tag="VF516_02795"
     CDS             complement(369..665)
                     /locus_tag="VF516_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8106623.1"
                     /translation="MPRVTFLVTNAPTRVIDVPPRATLLRAAVRARMPIGRSCRGVAV
                     CAACRVFIVEGSDAVEPMDELEARLASRVRLAEHERYACCARITGDVTVTTSYW"
     gene            complement(729..986)
                     /locus_tag="VF516_02800"
     CDS             complement(729..986)
                     /locus_tag="VF516_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106624.1"
                     /translation="MDEVLVQAAAFAHIQASPGCRGYTTWSSGRAGCGCCGWGMSARM
                     GDVGTPSSARFAEAAIARIGRAVWLGFLGVVVQALFEAVLP"
     gene            complement(1042..1197)
                     /locus_tag="VF516_02805"
     CDS             complement(1042..1197)
                     /locus_tag="VF516_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106625.1"
                     /translation="MSEIEGTNDIWGRPLKLLCGANLPPGAKGIGVLSLGEDGKEGTA
                     DDIKSWQ"
     gene            complement(1456..1596)
                     /locus_tag="VF516_02810"
     CDS             complement(1456..1596)
                     /locus_tag="VF516_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106626.1"
                     /translation="MAFTVVYDACVLFPAPLRDLLMRVAHTGLVRARWSNAILDECFR
                     NC"
     gene            complement(1596..2051)
                     /locus_tag="VF516_02815"
     CDS             complement(1596..2051)
                     /locus_tag="VF516_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family DNA-binding protein"
                     /protein_id="HEX8106627.1"
                     /translation="MAGPALKLPTQQTAQEAQAALRALSALPRKKPVRAIRVQPEGMA
                     DGVIVTVPREAFELFLEVLGQMANGNAVTIVPVHAELTTQQAADLLNVSRPYLIGLLD
                     SGKISFRLVGAHRRVKFSDLMAYKDADDRERQAALGELTDDAQKHGLGY"
     gene            complement(2071..3402)
                     /locus_tag="VF516_02820"
     CDS             complement(2071..3402)
                     /locus_tag="VF516_02820"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8106628.1"
                     /translation="MTTLVVVLGLVAVAGVPYLVWARMERRKQHRASIIHQDTLALGD
                     DIVPVSIHPEVDLDECIGSGACVRACPEKDILAITDGRARLLNPLSCIGHSACMQACP
                     VGAIKLVFGTATRGVELPRLTPSFETTQPGIFVIGELSGIGLIRNAMEQGRQAAATIA
                     RSSRRGRPGELDAIVVGAGPAGIGAALSLIAHGLKVQIVDQDRYGGTITHYPRSKVVM
                     TGSFELPGYGSVRRKTMTKEQLLTLWTDLRERIALDIREGIRVEAIHPDGPSWRLVGD
                     HVRGDGARWQDRAAAVVLALGRRGAPRELGVPGEQLAKVSYRLIEPEPFAHQHVLIVG
                     GGNAAADCAIALASAKLCASVSISYRRPELARLRASVRKDIERCFADGSIVPLLATEV
                     VSIDEKHVTLKTPDGVRQVINDRVIVQIGGQPPSQLLRTIGIELVEKRGEA"
     gene            complement(3399..4115)
                     /locus_tag="VF516_02825"
     CDS             complement(3399..4115)
                     /locus_tag="VF516_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106629.1"
                     /translation="MTRLVPALARVFALAWLAGCFQAHPPGQQELMGTDCYRCHQTDY
                     AATTAPVHRDTPQVFSTTCAGCHRTVNWKPALEGLHSDVFVIAAGKHANIACLACHDL
                     DSGQPSIKGANTNCLSCHPDDRALRDGHDGVTSATDAPYQYLAGVPNFCLQCHPAGTA
                     DIHPDNKFRRTGDHKVPCAQCHDRTAGPDTQGANVTCVEARCHHTVQAVTGGEGHTGS
                     AWTRALGDGASRNFCHQCHS"
     gene            complement(4112..>4380)
                     /locus_tag="VF516_02830"
     CDS             complement(4112..>4380)
                     /locus_tag="VF516_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106630.1"
                     /translation="QASFASWTAGELGAAYAPTRALDVALGYRPELLDYVASTGPVVL
                     HSAIADARLALSTALDLALSLTATTGSDRDALAVLATFAWRPLP"
BASE COUNT          634 a         1532 c         1569 g          645 t
ORIGIN      
        1 gcctgcgcgc gctggacgac cgcgatgtgc tcgccgaccg tcagcggcat cggcatcggt
       61 ccaccagccg cgcagcaggc agcgcctcgg tccgtcgcag cgtgcccagc agttgccggc
      121 gacacgagaa ggtgatcaca cgagccggcg cgggtgtccg cgggccggcg ccgcccggcc
      181 tgcttcgtcg aggcctcgcc tacagtccga accggatcgc cagccacagc ccgatcagca
      241 gcaccccggc gatggcaccc catgatggcg acgctcggca cgggtagcag accgcagccc
      301 tacctcccag ctggcaggta gggctgcggt cgcgccccgg cttgcgcgcg gtcgtcctgc
      361 cgcctgggtt accagtacga ggtcgtcacc gtgacatcgc ccgtgatgcg cgcgcagcat
      421 gcgtagcgct cgtgctcggc gagccggacg cgggacgcga gccgcgcctc gagctcgtcc
      481 atcggctcca ccgcgtcgga gccctccacg atgaacaccc ggcaggcggc gcacaccgcg
      541 acgccgcgac acgaccggcc gatcggcatc cgggcgcgca ccgcggcgcg cagcagtgtc
      601 gctcgcggcg gcacgtcgat cacccgcgtc ggggcgttgg tgaccaggaa cgtgacccgt
      661 ggcatctcgc ggtcacgcta gcgcggatct cggggcacgt ccggtggcgg ggatcacgcc
      721 acagcctcct acggcagcac cgcctcgaac agcgcctgca cgaccacgcc gagaaagcca
      781 agccagaccg cacggccaat gcgcgcgatc gccgcctcag cgaaccgcgc gctgctgggc
      841 gtgccgacgt cccccatccg cgccgacata ccccacccgc agcagccgca accggcgcgt
      901 cccgagctcc aggtcgtgta gccgcggcag ccgggcgagg cttggatgtg ggcgaacgcc
      961 gcggcctgca ccaggacctc gtccacaagg cgtgcgcacg agaatgtcgt agcggtccgg
     1021 ctactgcacg cgtttgcgca ctcactgcca ggacttgatg tcgtccgcgg tgccttcctt
     1081 gccgtcctcg cccaacgaga gcacgccgat acctttggcg ccgggcggca ggtttgcgcc
     1141 gcacaacaac ttgagcggtc gaccccagat gtcgttggtg ccctcgatct cgctcacggg
     1201 ggctgtacgg gccgaggtcg ctcatgctct ccgggcacgc tttgtcgggg tgcgccgagg
     1261 cccagcgcgg atagacctcg tacacatact tctccagctt cagtttgagc agttggaggc
     1321 caaacttgtc cagtttcctg ttgtctgagc gggctttcaa gaagccgacg gccacggcag
     1381 ccacgacgcc cacaacagcc acgactctga gccacatccg acaatcctac gccctcttcc
     1441 gggcgtgatc cactgctagc aattgcggaa acactcgtcc aggatcgcgt tggaccagcg
     1501 agcccgcacc agtccggtgt gcgcgacgcg catcaacagg tcgcgcaggg gcgccggaaa
     1561 taatacacag gcgtcgtaaa cgacggtgaa ggccatcagt acccgagtcc atgcttctgc
     1621 gcgtcgtcgg tgagttcgcc gagcgccgcc tgccgctcgc gatcgtcggc gtccttgtac
     1681 gccatcaggt ccgagaactt cacgcggcga tgagcgccta cgaggcggaa cgagatcttc
     1741 ccgctatcga gcaaaccgat cagatagggc ctcgacacgt tcaagagatc tgccgcttgt
     1801 tgcgtggtga gctcggcatg aaccggcacg atcgtgacgg cgttgccgtt cgccatctgt
     1861 cccagcactt ccaggaacag ctcgaaggcc tcgcgcggca cggtgacgat cacgccgtcc
     1921 gccatgccct ccggctgaac tcggatcgcg cgcacgggct tcttgcgcgg cagggccgaa
     1981 agcgcgcgca gggccgcctg cgcttcctgg gcggtctgct gagtgggcaa cttgagcgca
     2041 ggtcctgcca tgtggagagc gtgccatgcc tcacgcctcg ccgcgcttct cgaccagctc
     2101 gatgccgatc gtgcgcagca gctggctcgg cggctggccg ccgatctgga cgatcacgcg
     2161 gtcgttgatg acctggcgca cgccgtcggg cgtcttgagc gtgacgtgct tctcgtcgat
     2221 cgacacgacc tcggtggcga gcagcgggac gatgctgccg tcggcgaagc agcgctcgat
     2281 gtccttgcgc accgaggcgc gcagccgcgc cagctcgggg cggcggtagc tgatcgacac
     2341 gctcgcgcac agcttcgcgc tggccagcgc gatcgcgcag tcggccgccg cgttgccgcc
     2401 gccgacgatc aggacgtgct ggtgggcgaa cggctcgggc tcgatcaggc ggtacgacac
     2461 cttggcgagc tgctcgccgg ggacgccgag ctccctgggc gcgccgcgcc ggccgagcgc
     2521 gagcacgacc gcggcggcgc gatcctgcca gcgggcgccg tcgccgcgca cgtgatcgcc
     2581 gaccagccgc cagctcgggc cgtcggggtg gatcgcctcg acccggatgc cctcccggat
     2641 gtcgagcgcg atgcgctcgc ggaggtcggt ccacagcgtg agcagctgct ccttggtcat
     2701 ggtcttgcgc cgcacgctgc cgtagcccgg cagctcgaac gagccggtca tcacgacctt
     2761 ggacctcggg tagtgggtga tcgtgccgcc gtagcggtcc tgatcgacga tctggacctt
     2821 gagcccgtgg gcgatcaggg acagcgccgc gccgatcccc gcggggccgg cgccgacgac
     2881 gatcgcgtcg agctcgccgg ggcggccgcg ccggctggac ctcgcgatgg tcgcggcggc
     2941 ctgccggccc tgctccatcg cgttgcggat caggccgatc ccgctgagct cgccgatcac
     3001 gaagatgccg ggctgggtgg tctcgaagct cggggtcagc cgcggcagct cgacgccgcg
     3061 ggtcgcggtg ccgaacacca gcttgatcgc gccgaccggg caggcctgca tgcacgccga
     3121 gtggccgatg cacgacagcg ggttgagcag gcgggcccgg ccgtcggtga tcgccaggat
     3181 gtccttctcg gggcaggcgc gcacgcacgc gcccgagccg atgcactcgt cgagatcgac
     3241 ctcggggtgg atcgacaccg gcacgatgtc gtcgccgagc gccagcgtgt cctggtggat
     3301 gatgctggcg cggtgctgct tgcgccgctc catccgcgcc cagaccagat acgggacgcc
     3361 ggcgaccgcg accaggccga gcacgacgac cagcgtggtc acgagtggca ctgatggcag
     3421 aagttgcggc tcgcgccgtc gcccagcgcg cgggtccacg cgctgccggt gtggccctcg
     3481 ccgccggtga ccgcctgcac ggtgtggtgg caccgcgcct cgacgcaggt cacgttggcg
     3541 ccctgggtgt cggggcccgc cgtgcgatcg tggcactgtg cgcacggcac cttgtgatcg
     3601 ccggtgcgcc ggaacttgtt gtcggggtgg atgtcggcgg tgcccgccgg gtggcactgc
     3661 aggcagaagt tgggcacgcc ggcgaggtac tggtacggcg cgtcggtcgc cgaggtgacg
     3721 ccgtcgtggc cgtcgcgcag cgcccggtcg tcggggtggc acgacaggca gttggtgttg
     3781 gcgcccttga tcgacggctg tccgctgtcg aggtcgtggc acgccaggca ggcgatgttc
     3841 gcgtgcttgc cggccgcgat cacgaacacg tcgctgtgca ggccctcgag cgccggcttc
     3901 cagttgaccg tgcggtggca gccggcgcac gtcgtcgaga acacctgcgg cgtgtcgcga
     3961 tgcaccggcg cggtggtcgc ggcgtagtcg gtctggtggc agcggtagca gtcggtgccc
     4021 atcagctcct gctggccggg cgggtgagcc tggaagcacc cggcgagcca ggcgagcgcg
     4081 aacacccgcg cgagggcggg cacgagtcgg gtcatggcag gggcctccag gcgaaggtgg
     4141 cgagcaccgc gagcgcgtcg cgatcgctgc cggtggtggc ggtcagcgac agcgcgagat
     4201 cgagggcggt ggacagcgcg agccgggcgt cggcgatcgc cgagtgcagg accaccgggc
     4261 cggtgctggc gacgtagtcg agcagctcgg ggcggtagcc gagcgcgacg tcgagcgcac
     4321 gggtcggcgc gtaggcggcg ccgagctcgc ccgcggtcca gctcgcgaag ctggcctggc
//
LOCUS       DASVOR010000135         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_235000,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000135 DASVOR010000000
VERSION     DASVOR010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235000"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            421..>2079
                     /locus_tag="VF516_02835"
     CDS             421..>2079
                     /locus_tag="VF516_02835"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8106631.1"
                     /translation="MALAGTLAGSGCTGDTGPVGVKGDKGDPGAPGAQGSQGSQGNSA
                     PVPDTSDPAARLATRTAIKHVVILFGENVSFDHYFATYPDAQFPATRSNPPSTGVDGT
                     AFVALAGTPKPNGLKTPLDVNNNFMPLATNLLAPNNPNSAQPFRLNPSQASTQDQNHA
                     YGAEQAATNAGGMNLFPEKVGRGNPSFMSAPNDASLVMGYYDGNTVTALWSYAQHFAM
                     NDNSWTTQFGPSTPGALNLISGQTNGATVQTPGGSVVADGHGSFTVIGDPDPIGDVCS
                     NPAITQVQMSGKNIGDLLNAKNITWGSFMGGFDLTVTNPNGSYGCLRITNPTQGPFTG
                     ASVDYIPHHAWFQYYASTANLQHLRPSSLAAIGSSKTVDGKAEPANHNYDSHDFFEAL
                     AAGNLPAVSFVKAPAFQDGHGGYSNPIDEQTFIATTVNALQASKFWATTAVIIAYDDS
                     DGWYDHQAPPIVNPSSLDAIGNPASVTDAVDQLNNDGTHSPASGTGACTTTTAQQGQA
                     AATTALPGAAGMPVLGRCGYGTRMPLLVISPFAKKNFIDHTLTDQ"
BASE COUNT          363 a          787 c          642 g          287 t
ORIGIN      
        1 gcggcgcgat cgcggggacc tccccggcgg gcaacagcgc ggtggtctcg ccgctcggta
       61 gcgccgcctg gtgtgccggg tgtgccgggt gtgcgggctg ctcctcggcg gcgccggcca
      121 tcgccgacag tgtactcccg cggccggtgc agcgcggaat ccggcatgtc catgtggcgg
      181 cgcggtgacg gcagcatggc aatcccgcac gacgatctcc tcgcatcgtc acgggttgtc
      241 tcgatcgcgg ctcgatcatg ccgccgcact gtctctgtac cgtcaaacac gactcgtaca
      301 acggcctcct cgatccacac cgtcgcgcga cattgcgcac atctcccgct gccaccagga
      361 ggtccacgaa tgttcgacac tcgaagggtt gtgctccagg gaagcatcct ctcgggggcc
      421 ttggcgctcg ccggtacgct ggccggctcc ggatgcacgg gggacaccgg ccccgtcggc
      481 gtcaagggcg acaaggggga tccgggtgcg cccggcgcgc agggttccca gggctcccag
      541 ggcaattcgg ccccggttcc cgacacgagt gatccggccg ccaggctcgc gaccaggacg
      601 gcgatcaagc acgtggtgat cctcttcggc gagaacgtgt cgttcgatca ctacttcgcg
      661 acctaccccg acgcgcagtt ccccgcgacg cgcagcaacc cgcccagcac cggggtcgac
      721 ggcacggcgt tcgtcgcgct ggcaggcacc ccgaagccga acggcctcaa gacgccgctc
      781 gacgtcaaca acaacttcat gccgctcgcg accaatctgc tggcgccgaa caaccccaac
      841 tcggcgcagc cgttccggct caacccgtcg caggcctcga cccaggacca gaaccacgcc
      901 tacggcgccg agcaggccgc cacgaacgcc ggcggcatga acctgttccc cgagaaggtc
      961 ggccgcggca acccctcgtt catgagcgcg ccgaacgacg cgagcctggt catgggttac
     1021 tacgacggca acaccgtgac cgcgctgtgg agctacgccc agcacttcgc gatgaacgac
     1081 aactcgtgga ccacgcagtt cggtccgtcg acgcccggcg cgctcaacct gatctcgggc
     1141 cagaccaacg gcgccaccgt ccagacgccg ggtggcagcg tcgtggccga cgggcacggc
     1201 agcttcacgg tgatcggcga tccggatccg atcggcgacg tctgctccaa cccggcgatc
     1261 acccaggtcc agatgtcggg gaagaacatc ggcgatctgc tcaacgccaa gaacatcacc
     1321 tggggctcgt tcatgggggg cttcgacctg accgtcacca accccaacgg cagctatggc
     1381 tgcctccgga tcaccaaccc gacccagggg ccgttcaccg gcgcctcggt cgattacatc
     1441 ccgcaccacg cgtggttcca gtactacgcg tcgaccgcca acctgcagca cctgcggccg
     1501 agctcgctgg ccgcgatcgg ctccagcaag accgtcgacg gcaaggccga gcccgccaac
     1561 cacaactacg actcgcacga cttcttcgag gcgctcgccg ccggcaacct cccggcggtg
     1621 agcttcgtga aggcccccgc cttccaggac ggccacggtg gctactcgaa cccgatcgac
     1681 gagcagacct tcatcgccac cacggtcaac gcgctgcagg cgagcaagtt ctgggcgacg
     1741 acggccgtga tcatcgccta cgacgactcc gacggctggt acgaccatca ggcgccgccg
     1801 atcgtcaatc cgtcgtcgct cgacgcgatc ggcaacccgg cgagcgtgac cgacgccgtc
     1861 gatcagctca acaacgacgg cacccacagc ccggccagcg gcaccggcgc ctgcaccacg
     1921 accaccgccc agcagggcca ggccgccgcc acgacggcgc tgcccggcgc cgccggcatg
     1981 ccggtgctcg gtcgctgcgg ctacggcacg cgcatgccgc tgctcgtgat ctcgccgttc
     2041 gcaaagaaga acttcatcga ccacacgctg accgaccag
//
LOCUS       DASVOR010000136         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205879,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000136 DASVOR010000000
VERSION     DASVOR010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205879"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VF516_02840"
     CDS             complement(<1..389)
                     /locus_tag="VF516_02840"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8106632.1"
                     /translation="MDKLEHGEQTIEGPGITRRRFVGYLIAAPTLVAAADLVFENTAR
                     AALPTVQAVDAFDLTDALTAAAAPTSQLISVEIRSDGTAAFALPRAEVGQGITTAVAM
                     TIADEMGMPLDKVHVTLADARPELVWN"
     gene            925..1506
                     /locus_tag="VF516_02845"
     CDS             925..1506
                     /locus_tag="VF516_02845"
                     /inference="COORDINATES: protein motif:HMM:NF015858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajG family lipoprotein"
                     /protein_id="HEX8106633.1"
                     /translation="MRVPSIAFLVAAACVAGAACGGGDSNIVNLPLRWQGVEALPRAS
                     GSVGRALSAAPFALGLRDLRPDPTAVGTYQDTGFVVRTTDNVGQFCSDRLGEMLVRAG
                     ARLEPGAPVTVEADLLDYQVVEGKRFVGTVRMRTTVKRGASTVWSKTYTGSNERWGRT
                     HNPENFNEALSNSLADAAQQLLQDNEFAKSLGG"
     gene            1512..>1843
                     /locus_tag="VF516_02850"
     CDS             1512..>1843
                     /locus_tag="VF516_02850"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8106634.1"
                     /translation="MAGSGPDVLVIGAGPAGSVAAALLAQRGYQVLVQERQHFPRFSI
                     GESLLAYSTQVLQEAGLLDAVLAGGFQFKNGATFVCDELYSEFNFANKFTAGCSFTFQ
                     VLRSEFDHL"
BASE COUNT          297 a          623 c          636 g          287 t
ORIGIN      
        1 tggttccaca cgagctccgg ccgcgcatcg gccagcgtca cgtggacctt gtcgagcggc
       61 atgcccatct cgtcggcgat cgtcatcgcc accgcggtcg tgatgccctg cccgacctcg
      121 gcgcgcggca gcgcgaaggc cgcggtcccg tcggagcgga tctcgaccga gatcagctgc
      181 gacgtcggtg ccgccgcggc ggtcaacgcg tcggtcaagt cgaaggcgtc gacggcttgc
      241 acggtcggca gcgcagcccg tgccgtgttc tcgaagacca ggtcggccgc ggccacgagc
      301 gtcggcgcag cgatcaggta gccgacaaaa cgacgccgcg tgatgcccgg gccctcgatc
      361 gtctgttcgc catgttcgag cttgtccatc tggacctcct ctcggcggcg tccagcagcg
      421 acaccttcag gtcgatgatg cgaaaaggca gctcagagac cgcgttgcag atggtaagag
      481 aagaatgtct ggatcaaatc ggttttacgc gattctaacg ctgtgtgacg cgccgcatgc
      541 cgctcgccgg ttcgatcggc acaactccga cgggttcgac tgcgagatcg ctcacacaaa
      601 ctcgctcggg cgcaccatcc tgtccaccgc acacaaccaa tgtaaaacga gcggcacgca
      661 gatgcggtat ccgatcgact gtgcgagatt ggctccgttc gaccaggaca ccggctcgtc
      721 caggggtgcg gtgcccgcgg cgcgcccgga ggttcgagcc agagctcgag caacgaggta
      781 cagcgcaatc gccgggtcga gatctacatc gagcgcgacc gcgtgctgca gcgtccgatc
      841 gcgccagcac agaccgcaac gctccgcgac cgaagtggtc gaaccacttc atcccgccga
      901 cgagttgtgg tatggcttgg cgcggtgagg gttccatcca tcgcgttcct ggtggccgca
      961 gcctgcgtcg ccggcgcagc ttgcggcggc ggcgacagca acatcgtgaa cctccccctg
     1021 cgatggcagg gcgtcgaggc gctgcccagg gcgagcggat cggtggggcg cgcgctgtcg
     1081 gcggcgccgt tcgcgctcgg gctgcgcgac ctgcgcccgg atccgaccgc ggtcgggacg
     1141 taccaggaca ccgggttcgt ggtccggacc accgacaacg tcggccagtt ctgcagcgac
     1201 cggctcggcg agatgctggt ccgcgccggc gcgcgcctcg agccgggggc gcccgtcacc
     1261 gtggaggccg atctgctcga ctaccaggtc gtcgagggca agcggttcgt gggcaccgtg
     1321 cggatgcgca cgacggtcaa gcgcggcgcc agcacggtct ggtccaagac ctacaccggc
     1381 tcgaacgagc gctggggccg cacccacaac ccggagaact tcaacgaggc gctgtcgaac
     1441 tcgctcgccg atgcggcgca gcagctgctg caggacaacg agttcgcgaa gtcgctgggg
     1501 ggctagcgcg cgtggccggg agcgggccag acgtcctggt catcggcgcg ggaccggcgg
     1561 gctcggtggc cgccgcgctg ctggcacagc gcgggtacca ggtcctggtg caggagcggc
     1621 agcacttccc gcggttctcg atcggcgaga gcctgctcgc gtacagcacg caggtcctgc
     1681 aggaggccgg cctgctcgac gccgtgctcg ccggcgggtt ccagttcaag aacggcgcca
     1741 cgttcgtgtg cgacgagctg tactcggagt tcaacttcgc caacaagttc accgccgggt
     1801 gctcgttcac gttccaggtg ctgcgctcgg agttcgacca cct
//
LOCUS       DASVOR010000137         2607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_89022,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000137 DASVOR010000000
VERSION     DASVOR010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2607
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89022"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VF516_02855"
     CDS             <1..261
                     /locus_tag="VF516_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106635.1"
                     /translation="ARDEAVVLDLLQRQQWDQARTALHQLSARDPSSKRIRALESYAR
                     GREAQLDRRLDDARVELQDALELDPDLQAAKTALTELFTRRR"
     gene            280..1830
                     /gene="dnaK"
                     /locus_tag="VF516_02860"
     CDS             280..1830
                     /gene="dnaK"
                     /locus_tag="VF516_02860"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8106636.1"
                     /translation="MGRVIGIDLGTTNSCVAVLEGGEPTVIHNQEGGRTTPSMVSWNV
                     DGDVVVGAASKRQMITNPQRTVFGAKRLIGRKMHDREVQSLVARLPYKVVPAKNGDAW
                     VEVAGTPRSPQEISAHVLAKMKRVAEDYLGEPVFEAVVTVPAYFDDVQRQATKDAGAI
                     AGLTVRAILNEPTAAALAYGAQHQHNQRLAVFDLGGGTFDISILAIENGVFEVLATHG
                     DTSLGGDDFDRVIIDLLADEFAAEHGINLRDDSVALQRLKEAAERAKIELSSAMTTDV
                     NLPFIAAGPAGPVHLMRELERGQLERACRHLIDRLDAPCRRALADAHCTPADVDQVLL
                     VGGMSRMPAVQERAVQIFGKPASRGVNPDEIVAMGAAVHSGIMGGELQEVVLLDVTPH
                     DMGVKVAGDRMSVVITANTSIPTRAKKIFATTEDNQTYVAIEIYQGEHELASRNRRLG
                     RFILGDLRPAPRGQTKVEVSFTMDADGILEITAAEIGTGRAASVQIEASSGLTAEEIQ
                     RLGQRLGT"
     gene            1990..2130
                     /locus_tag="VF516_02865"
     CDS             1990..2130
                     /locus_tag="VF516_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106637.1"
                     /translation="MDKKPSESTTDEKPKETPPPPGPPKKKNLSLDITDVSEVLERKI
                     SP"
     gene            2252..>2607
                     /locus_tag="VF516_02870"
     CDS             2252..>2607
                     /locus_tag="VF516_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106638.1"
                     /translation="MRTAIASDAELYIAREDARDAADQVALARSVFLPRLSGEIAGTR
                     NAQPPSATSFAAIDAIAAATLGISGRIDTGMTYSVSAGITRQDRDDPFAKTYDVATTT
                     AVRAEIVQPLRRGAFAA"
BASE COUNT          431 a          962 c          869 g          345 t
ORIGIN      
        1 gcgcgtgacg aggccgtcgt cctcgatctt ctccagcgcc agcaatggga tcaggcgcgc
       61 accgcgctcc accagctgtc ggcgcgcgat ccgagctcga agcggatccg tgcgctggag
      121 tcctacgccc gcggccgaga ggcccagctc gatcgccggc tcgacgacgc gcgcgtcgag
      181 ctgcaggacg cgctcgagct cgatccggat ctccaggccg ccaagaccgc gctgaccgag
      241 ctgttcacac gccgaagata ggtatacttc cgacgaggca tgggccgcgt catcgggatc
      301 gatctcggca ccacgaattc gtgcgtggcg gtactggagg gcggggagcc gacggtgatt
      361 cacaaccagg agggcgggcg gaccacgccg tcgatggtgt cgtggaacgt cgacggcgac
      421 gtcgtggtcg gcgcggcgag caagcgccag atgatcacca acccgcagcg caccgtgttc
      481 ggcgccaagc gcctgatcgg ccgcaagatg cacgaccgcg aggtccagtc gctggtcgcc
      541 cggctgccct acaaggtcgt ccccgccaag aacggtgacg cctgggtcga ggtcgccggc
      601 accccgcgct cgccgcagga gatctcggcc cacgtgctcg ccaagatgaa gcgggtcgcc
      661 gaggactacc tgggcgagcc ggtgttcgag gccgtggtca ccgtgccggc gtacttcgac
      721 gacgtccagc gccaggccac caaggacgcc ggcgcgatcg ccgggctcac cgtccgcgcg
      781 atcctcaacg agccgaccgc cgcggcgctg gcctacggcg cccagcacca gcacaaccag
      841 cggctcgccg tgttcgacct cggcggcggc acgttcgaca tctcgatcct cgcgatcgag
      901 aacggcgtgt tcgaggtcct cgccacccac ggcgacacct cgctcggcgg cgacgacttc
      961 gatcgcgtga tcatcgacct gctcgccgac gagttcgcgg ccgagcacgg catcaacctg
     1021 cgcgatgact cggtcgcgct gcagcggctc aaggaggccg ccgagcgcgc caagatcgag
     1081 ctgtcgtcgg cgatgaccac cgacgtcaac ctgccgttca tcgccgccgg cccggccggc
     1141 ccggtccacc tgatgcgcga gctcgagcgc ggccagctcg agcgcgcctg ccgccacctg
     1201 atcgaccggc tcgacgcccc gtgccgccgc gccctggccg acgcccactg cacgccggcc
     1261 gacgtcgacc aggtcctgct cgtcggcggc atgtcccgga tgcccgcggt ccaggagcgc
     1321 gcggtccaga tcttcggcaa gccggccagc cggggcgtca accccgacga gatcgtcgcg
     1381 atgggcgccg ccgtgcactc cggcatcatg ggcggcgagc tgcaggaggt cgtgctgctc
     1441 gacgtcacgc cgcacgacat gggcgtcaag gtcgccggcg accgcatgtc ggtcgtgatc
     1501 accgccaaca ccagcatccc gacccgcgcc aagaagatct tcgcgaccac cgaggacaac
     1561 cagacctacg tcgcgatcga gatctaccag ggcgagcacg agctcgcgtc gcgcaaccgc
     1621 cggctcggcc ggttcatcct cggcgacctc cgccccgcgc cgcgcggcca gaccaaggtc
     1681 gaggtcagct tcacgatgga cgccgacggc atcctcgaga tcaccgccgc cgagatcggc
     1741 accggccgcg ccgccagcgt ccagatcgag gcctccagcg gcctcaccgc cgaggagatc
     1801 cagcgcctcg gccagcgcct gggcacatga cgcagcgcgc ggcagctccg aagaccggcc
     1861 atccgttttc acagatactc gacgagccgc atctcttgtg tgcgcgtccg ctcatgtgcc
     1921 catggtctgc attgacagcc tcgacgattg gtctagtctc gtctcgcgat ttccaaccga
     1981 ggattggcaa tggacaagaa accatcagag agcaccaccg acgaaaagcc caaggagacg
     2041 ccgccgccgc ccgggccgcc caagaagaag aacctgtcgc tggacatcac cgacgtgtcc
     2101 gaggttctcg agcgcaagat ctcgccatag cggtatgcgt cgcttgccag gccgcgccgc
     2161 agcgatactc ggaggcgttg cgctcgcgct gggagcggga tcggagcgtg cggcggcgca
     2221 ggaggcccac gagctctcgc tcgccgcagc cgtgcgaacc gcgatcgcga gcgacgccga
     2281 gctctacatc gcccgcgagg acgcgcgcga cgccgccgac caggtcgcgc tggcgcgctc
     2341 ggtgttcctg ccccggctgt cgggcgagat cgcggggacg cggaacgccc agccgccctc
     2401 ggcgacgtcg ttcgccgcga tcgacgcgat cgccgcggcg acgctcggga tctccggccg
     2461 gatcgacacc gggatgacgt acagcgtctc cgcagggatc acacggcagg accgcgacga
     2521 tccgttcgcg aagacctatg acgtggcgac gaccaccgcc gtgcgcgccg agatcgtcca
     2581 gccgctgcgc cggggggcgt tcgcggc
//
LOCUS       DASVOR010000138         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_556595,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000138 DASVOR010000000
VERSION     DASVOR010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556595"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1633
                     /locus_tag="VF516_02875"
     CDS             <1..>1633
                     /locus_tag="VF516_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106639.1"
                     /translation="MSEQMRKAWLDFLDAETAVQPVLLVLEDLHWGDFGTVRFLDTAL
                     RDRNQRPWMVLALARPEVFEVFPRLWAERQNVQEIRLQELSRKAGERLVRQVLGDRVD
                     PGTMERLVQQADGNAFYLEELIRAAAEGKDHALPDTVLAMVETRLGRLALEARRVLRA
                     ASVFGEVCWEGGVAVLLAGAMTAAEVAQWLAALVDQELLAVRPGSQFPHERELAFRHA
                     LLREGAYATLTGDVRRLGHRLAGEWLERHGEGDPMVLAGHFERGGDAAHAAAYYLRAS
                     EQAYHVLDADATMARANLGLACAPPPELRIALFGMRCEACGYRLDWVGSTMPDAEELM
                     RSAPPGSIHWTQGVVVHIQGSLISGRTPDLLATIGLLRDVDPTPEALGKMALALLVSI
                     FLLDSFGRVGEGTAMEQRFRAIAESAAHRDPTLDFWWHVALAMRASYAHEDLGTALDH
                     SDAIQPIFETTGGELKFINLHLYRGLNLWFLGASAAAQHHLEQIVALDDMLSLVGYLR
                     RFALAWLLADRGALDQAHALADQLSEYGRARQAALE"
BASE COUNT          205 a          603 c          596 g          229 t
ORIGIN      
        1 atgagcgagc agatgcgcaa ggcgtggctc gacttcctcg acgccgagac cgccgtccag
       61 cccgtcctgc tcgtgctcga ggacctgcac tggggcgact tcggcaccgt gcggttcctc
      121 gacaccgcgc tgcgcgatcg caaccagcgg ccgtggatgg tgctcgcgct cgcccgcccc
      181 gaggtgttcg aggtgttccc caggctgtgg gccgagcgcc agaacgtcca ggagatccgc
      241 ctccaggagc tgagccgcaa ggccggcgag cggctggtcc gccaggtgct cggcgaccgc
      301 gtcgaccccg ggacgatgga gcgcctggtc cagcaggccg acggcaacgc gttctacctc
      361 gaggagctga tccgcgccgc cgccgagggc aaggaccacg cgctgccaga caccgtgctg
      421 gcgatggtcg agacccggct cggccggctc gcgctcgagg cccgccgcgt gctgcgcgcc
      481 gccagcgtgt tcggcgaggt gtgctgggag ggcggcgtcg ccgtcctgct cgcgggcgcg
      541 atgaccgcgg cggaggtcgc gcagtggctc gcggcgctgg tcgatcagga gctcttggcg
      601 gtgcggcccg gcagccagtt cccccacgag cgcgagctcg cgttccgcca cgccctgctg
      661 cgcgagggcg cctacgcgac cttgaccggg gatgtccggc ggctcggcca ccggctcgcc
      721 ggcgagtggc tcgagcgcca cggcgagggc gacccgatgg tgctcgccgg ccacttcgag
      781 cgcggcgggg atgccgcgca cgccgcggcc tactacctgc gggcctccga gcaggcctat
      841 cacgtgctcg acgccgacgc cacgatggcc cgcgccaacc tcggtctggc gtgcgcgccg
      901 ccgcccgagc tccggatcgc gctgttcggc atgcgctgcg aggcctgcgg ctaccgcctc
      961 gactgggtcg gctcgacgat gcccgacgcg gaggagctca tgcgctcggc gccgccgggc
     1021 tcgatccact ggacccaggg cgtggtcgtc cacatccagg gctcgctgat ctccggccgc
     1081 acgcccgatc tgctggcgac gatcggcctt ctgcgcgacg tcgacccgac ccccgaggcg
     1141 ctgggcaaga tggcgctggc gctgctggtc tcgatcttcc tgctcgacag cttcgggcgg
     1201 gtcggcgaag gcaccgcgat ggagcagcgg ttccgcgcga tcgccgagtc ggccgcgcac
     1261 cgcgacccga cgctggactt ctggtggcac gtcgcgctcg cgatgcgggc gtcgtacgcg
     1321 cacgaggacc tggggaccgc gctggaccac agcgacgcga tccagccgat cttcgagacc
     1381 accggcggcg agctcaagtt catcaacctg cacctgtacc gcgggctcaa cctgtggttc
     1441 ctcggcgcgt ccgcggcggc ccagcaccac ctggagcaga tcgtggcgct cgacgacatg
     1501 ctgtcgctgg tcggctacct gcggcggttc gcgctggcct ggctgctcgc cgaccgcggc
     1561 gcgctcgatc aggcccacgc gctggccgac cagctcagcg agtacgggcg cgcgcggcag
     1621 gccgcgctgg agg
//
LOCUS       DASVOR010000139         2584 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439602,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000139 DASVOR010000000
VERSION     DASVOR010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439602"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            107..1366
                     /locus_tag="VF516_02880"
     CDS             107..1366
                     /locus_tag="VF516_02880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8106640.1"
                     /translation="MSRASHAAAFIAGAAVASAIVAHAVPRADVARFKSIDAFAQALA
                     TVEANYVDPIDERKLLYDAARGMLHNLDPHSTFLPPSRYQHLRQDTEGEFGGVGVTLA
                     PGEVDDARPSVPPYPTIDAIVHGSPADLAGLQLDDAVVAIDGEATAEVGREKRDAGAW
                     EARLRGPSGTRVVISVLRTGWRDPRPFTVVRAQVKQPTVRHDVLDKRIGYLAITRFSE
                     ATSADVLAGLTKLRQQGALDVLVLDLRNDPGGLVDQSIAVADQFLDSGTIVTIRGRHG
                     SVETQVAHKGGIAVGVPVIALVDQGTASAAEILAAALRDHGRAQLVGVPTYGKGTVQT
                     FYDLDDGSGLKLTTARYYTPAGKLLESNGITPDVLVEPFAPEEIVAGESNGSGASPGN
                     DATIKPPAEDPQLAAAVRLARQALQKR"
     gene            1467..>2584
                     /locus_tag="VF516_02885"
     CDS             1467..>2584
                     /locus_tag="VF516_02885"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8106641.1"
                     /translation="MFKLVIQDDEGKTTIVPLIRDEITIGRKEGNTIRLTERNVSRRH
                     ARILRNNGEVHIEDLGSYNGIRVNNARIAERVSLRVSDQVQIGDYKLYLKAEGLDQVD
                     DSRTTPIERVEAGPTDSMPVVAVPPSVITPAIATPVPTVPPMIGDLNRPNRTMVAMSD
                     TDPGRSVGPVTAAAVAALTAPGGYGKLVVLSSNFAGKEFELSRPQMIIGRTDENDIVI
                     NHRSISRNHAKLTREPETGRYTISDLQSSNGVRVNGSDYGKVELRRGDVIDLGHVRLR
                     FIEAGEDFVFARDAVITDVPEAGGRRGMVVALIAAIVVLVASVAVWTWRTHTDEPNRH
                     GSATGSSGMAGNLPADADETPAAAPAPADTGAPAVAAVL"
BASE COUNT          430 a          951 c          871 g          332 t
ORIGIN      
        1 ccatcgcgca gcaccccaga ccgcgcaccc cagaccgcgc accccacatc gcgcagcacc
       61 ccccggccgc gcagcaccgg gccgcgacat gctctagact gaccgcgtgt cgcgcgcatc
      121 gcatgccgcc gcgttcatcg cgggtgccgc agttgccagc gccatcgtcg cccacgccgt
      181 gccgcgtgcc gacgtggccc ggttcaagtc gatcgacgcg ttcgcccagg cgctcgcgac
      241 ggtcgaggcc aactacgtcg acccgatcga cgagcgcaag ctcctgtacg acgccgcccg
      301 cggcatgctc cacaacctcg acccgcactc gacgttcctg ccgccgagcc gctaccagca
      361 cctgcgccag gacaccgagg gcgagttcgg cggcgtcggc gtgacgctgg cgcccggcga
      421 ggtcgacgat gcgcggccca gcgtgccccc gtacccgacg atcgacgcga tcgtgcacgg
      481 ctcgccggcc gatctcgccg ggctgcagct cgacgacgcg gtggtcgcga tcgacggcga
      541 ggccaccgcc gaggtcggcc gcgagaagcg cgacgccggc gcctgggagg ccaggctgcg
      601 cggcccgtcc ggcacccggg tcgtgatcag cgtgctgcgc accggctggc gcgatccgcg
      661 gccgttcacc gtcgtccgcg cccaggtcaa gcagccgacc gtgcgccacg acgtgctcga
      721 caagcggatc ggctacctcg cgatcacccg gttctcggag gcgacctcgg ccgacgtgct
      781 cgccgggctg accaagctcc gccagcaggg cgcgctcgac gtgctcgtcc tcgacctgcg
      841 caacgacccc ggcggcctgg tcgaccagtc gatcgcggtc gccgaccagt tcctcgacag
      901 cgggacgatc gtcacgatcc gcggccggca cggcagcgtc gagacccagg tcgcgcacaa
      961 gggcgggatc gcggtcggcg tcccggtgat cgccctggtc gaccagggca ccgcgtccgc
     1021 ggcggagatc ctcgcggcgg cgctgcgcga ccacggccgc gcgcagctcg tgggggtccc
     1081 gacctacggc aagggcaccg tccagacgtt ctacgatctc gacgacggct ccggcctcaa
     1141 gctgaccact gcgcgatatt acacacccgc tggtaagttg cttgaatcca acgggataac
     1201 acccgatgtc ctggtcgagc cgttcgctcc ggaagagatc gtcgcaggcg agagcaacgg
     1261 tagtggcgcg agccccggga acgatgccac aatcaaacca cccgccgagg atccccagct
     1321 ggccgctgcg gtccggttgg cccggcaggc gcttcagaag cggtagcaag tagtcggtgt
     1381 gacagttgtt taccaagagc ttggactgat acaaccaacg aacgtgggcg tttaggcaac
     1441 gctaaccgcg gggacgcaat ccgacgatgt tcaagctggt gatccaggac gacgagggaa
     1501 agacgacgat cgtccctctg atccgcgatg agatcacgat cggccgcaag gaaggcaaca
     1561 cgatccgcct caccgagcgg aacgtatcgc ggcgccacgc gcgtatcctg cgcaacaacg
     1621 gcgaggtcca catcgaggac ctcggcagct acaacggcat ccgggtcaac aacgcgcgga
     1681 tcgccgagcg cgtgtcgctg cgggtcagcg accaggtcca gatcggcgac tacaagctgt
     1741 acctcaaggc cgagggcctc gaccaggtcg acgattcacg gaccacgccg atcgagcggg
     1801 tcgaggccgg gccgaccgat tcgatgcccg tggtcgccgt cccgccctcg gtgatcacgc
     1861 cggcgatcgc gaccccggtg ccgacggtgc cgccgatgat cggcgatctg aaccggccga
     1921 accgcacgat ggtcgcgatg tcggacaccg atccgggccg cagcgtcggg ccggtcaccg
     1981 cggcggcggt cgccgcgctg accgcgccgg gcggctacgg caagctggtg gtcctgtcgt
     2041 cgaacttcgc cggcaaggag ttcgagctgt cgcggccgca gatgatcatc ggccgcaccg
     2101 acgagaacga catcgtcatc aaccaccgct cgatcagccg caaccacgcc aagctgaccc
     2161 gcgagccaga gaccggccgc tacacgatca gcgacctgca gtcgtccaac ggcgtgcggg
     2221 tcaacggctc ggactacggc aaggtcgagc tgcggcgcgg cgacgtgatc gacctcggcc
     2281 acgtccggct gcgcttcatc gaggccggcg aggacttcgt gttcgcgcgc gacgcggtga
     2341 tcaccgacgt ccccgaggcc ggtggccggc gcggcatggt ggtcgcgctg atcgccgcga
     2401 tcgtcgtgct ggtcgccagc gtcgcggtct ggacctggcg cacgcacacg gacgaaccga
     2461 accgccacgg cagcgcgacc ggcagctcgg gcatggccgg taacctgccg gccgacgccg
     2521 acgagacccc cgcggcggcg ccggcgccgg ccgacactgg cgccccggcc gtcgcggccg
     2581 tgct
//
LOCUS       DASVOR010000140         7878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_205883,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000140 DASVOR010000000
VERSION     DASVOR010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7878
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205883"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2570
                     /locus_tag="VF516_02890"
     CDS             <1..2570
                     /locus_tag="VF516_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106642.1"
                     /translation="EGVPLQVSASSDDGFALFAATASKVSLPLDRLLKAHGPGVPAPS
                     ALTVNQLSARVSRSAGYDLTALLAGEPEPWVVAIGKAQLTFSNLSLSLDHPASGQVTG
                     AFCGKVRFVDDIVLEASYQVPGSFFIRGSLPPLRLSKVIDKLCNQLVKLPKDFDFGLE
                     YASIILQGQQGSYAFQLLTTVKDFGTLVFEARETSGSCGFAFGLDLQAPKLSRVPGLS
                     ALAALDDNFQLQKFLLVASSIVQPSFQLPDAAQFNNPRLATKKVALPAGTSGVVAGLN
                     IFAQWALDPANKQHKLLSSLLGLSGTQQVTIQIPEDPRNGTRLFFAHSGTLMKHPFSY
                     EVGVLLQNGLPSLFMTGTLIAEIQKQPQTFDVTTLFVAGGAFLSADMKGPKPIDCKLF
                     KLSNVGLEIGVDWEGIPSLGVTATIDVKSFESSVAVFFDSADPSKSLIAGAVSDLTLK
                     EVVDTLLGGPVKSSIDGVLASIAVRGTQQFSIPATLADDLAQLRLDSIAAAFQSAGKI
                     KIPASASQVLLSANVPGKVWHLTDLTTMRHYQLTKQDTSIVVSLEAQFYFAPQATSIG
                     TIVFPQGFFINGALTFLGFHAEATVNISAGKGISADAHMDKIEIGKGGVFALTSADGK
                     RGPELSISTMTQPNNPVAEFRTPHMYISGAAQLLGIRNQVLARFSTQGMLLALKGALA
                     PGAEFDLDIQCAASGVTVDGDVKAGIGTIDLGQLGKVKVNTDVEGTLGVRASAQQVAV
                     AAEASFSFLGENKEIAKFALDTKPDALVHFADTLKSKAEALIREEFKDSTRWANAVKS
                     GAVEGVTDTEKVLKNVYGKSDKEAKAVASDIAGGSKAAAKTVTKSASKFGKKLKKVF"
     gene            complement(2698..3384)
                     /locus_tag="VF516_02895"
     CDS             complement(2698..3384)
                     /locus_tag="VF516_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106643.1"
                     /translation="MRVTGARGSFATDRVTGAVLAVRTAMRPDQYPEPLSQSAEEHSR
                     VASDYLVRSGVPTSEVSGTHVTTTMAGGMSRDGKLAPWSQRLLWYSTHLERSVGGVPV
                     EGSFAFAALDTSGAAITEGVYWPSIPADVVARAQELSRRLGSPDTRASFFEPVLKAHP
                     EIDKAAGTVKIVHTSAGYPGRFEVQAVYSVIVRNPNRGKAKTLRFDGSGARVVLGDER
                     ATGADSPKTR"
     gene            complement(3798..4010)
                     /locus_tag="VF516_02900"
     CDS             complement(3798..4010)
                     /locus_tag="VF516_02900"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX8106644.1"
                     /translation="MAGFGFYLGGEKLRDLKCRASWLWPDALVLVATIRALTYQGSIP
                     VKELALSNADAIDRRHTWHSVPVSAI"
     gene            4195..5568
                     /locus_tag="VF516_02905"
     CDS             4195..5568
                     /locus_tag="VF516_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106645.1"
                     /translation="MRLFFMTTLLAACANASGSATAEGALSASIVCLAPIGGWNVKLQ
                     GSTWNDEADAIAADNSDCAIYLAGDTVNVLPGATSFGGQDAFLIRYNSSGSVVWTRQF
                     GSLLGSDEAEWATGVAVSANHEIYVTGCTTGQMTNAPETNAGGTDVFLAKYDHDGNQL
                     WIHQFGTTTDDCSTGVAVGVSGGVFVVGSTHAFLPGSLSAYFGGADAFLVKYSSAGVR
                     QFTQMLGSPVDDEASAVAIGPSGNVYVTGDTEEDLPRFANFPDEQGTRNGGADIFLAK
                     YDPTTGAPIWLRQRGTSGEDRANGVVVNDSNEVFLTGYTNGWFDGIPTHGLEDIVVIS
                     YDTAGTWRWTDQRGSLGDDRGTAITVDSSGTPFVTGYASSTVDGEPYAGGTSDVVVIK
                     YGRAGVHRWTADLGERDNERPTGIAAGVGDDLFVGGFTDGNLGNALGFGENDAFVARF
                     SQTGDLD"
     gene            complement(5582..7267)
                     /locus_tag="VF516_02910"
     CDS             complement(5582..7267)
                     /locus_tag="VF516_02910"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEX8106646.1"
                     /translation="MIPPHSEAVADPLATAKPRPIVEIAARLGLTADEIEPYGRIKAK
                     LPLARIDLARAAKSKLILVSAVTPTPAGEGKTTTSIGLADGLSRLGKRAVAVLREPSL
                     GPVFGMKGGATGGGRARLIPHADINLHFNGDFAAVEKAHNLLAAVVDNHLHSKKSTLD
                     LDPLTIRWKRVIDMNDRALRRIVIGLGGSGSGVPREAGFDITAASEVMAILCFAQSTA
                     DLKERLGNIFIGFSRGRRPVYARDLKVHGAMTALLREAIQPNLVQTLEGTPAILHGGP
                     FGNIAQGTNSVVATRLGLSLADWVVTEAGFGFDLGGEKFLDLKCRASGLWPDALVLVA
                     TIRALKYQGGIPVKELAVPNADAIRRGFPNLARHLDNARFFGLRPVVVINRFAADHDD
                     ELALVRELCAKHEGTVVASDHFAHGGEGAMDAAAAVVAAAESGTRAPKFAYPLDAPVE
                     AKIEAIARGLYGAGEIALAAKARTDLKTIAELGLTNLPICMAKTQYSFSEQANLLGRP
                     EGFTLAVREIEIAAGAGFLIPICGDIMRMPGLPDAPAAEHIDLDDAGEVTGLT"
     gene            7464..>7878
                     /locus_tag="VF516_02915"
     CDS             7464..>7878
                     /locus_tag="VF516_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106647.1"
                     /translation="MFGFSAVASAVYRVTTESNVYILGFHEERGRKYVVVRGLPGTDR
                     EHVVLRDSDPRIGEVSMFEVPPHQWIGKNMEIATMSSSEVTAVTLETDRAAISSVGGD
                     SPSARSAWSPATGETMPGFPSRPPGLPELPGVRAGL"
BASE COUNT         1307 a         2659 c         2674 g         1238 t
ORIGIN      
        1 tcgagggcgt accgctgcag gtctcggcga gcagcgacga cggcttcgcc ctgttcgcgg
       61 ccaccgcgag caaggtgagc ttgcccctgg accggttgct caaggcccac ggtccgggcg
      121 tcccggcgcc gagcgcgctc accgtcaacc agctcagcgc tcgggtctcg cgcagcgccg
      181 gctacgatct gacggcgctg ctcgccggcg agcccgagcc ctgggtggtc gcgatcggca
      241 aggcgcagct gacgttcagc aacctgtcgc tgtcgctcga ccaccccgcg tcgggtcagg
      301 tcaccggcgc gttctgcgga aaagtccgct tcgtggacga catcgtcctg gaagccagct
      361 accaggtccc cggcagcttc ttcatccgcg gcagcctgcc gccgctacgg ttgagcaagg
      421 tcatcgacaa gctgtgcaat cagctggtca agctgcccaa ggacttcgac ttcggactcg
      481 agtacgcctc catcatcctg caggggcagc agggcagcta cgccttccag ctcctgacca
      541 cggtgaagga tttcggcacg ctggtgttcg aagcccgcga gaccagcggc tcctgcggct
      601 tcgcgttcgg cctcgatctg caggccccca agctgtcccg cgtccccggg ctctcggcgc
      661 tggcggcgct ggacgacaac ttccagctgc agaagttcct cctcgtcgcg tcctcgatcg
      721 tgcagccgag cttccagctg cccgacgccg cgcagttcaa caacccacgg ctggcgacca
      781 agaaggtcgc gctgcccgcc ggcaccagcg gcgtggtcgc gggtctgaac atcttcgcgc
      841 agtgggcgct cgaccccgcc aacaagcagc acaagctgct gtcgtcgctg ctcgggctct
      901 cggggaccca gcaggtcacg atccagatcc ccgaggatcc gcgcaatggc acgcggctgt
      961 tcttcgcgca cagcgggacc ttgatgaagc acccgttcag ctacgaggtc ggcgtgctgc
     1021 tgcagaatgg cctgccaagc ctgttcatga ccgggaccct catcgcggag atccagaagc
     1081 agccccagac cttcgacgtc accacgctgt tcgtcgccgg cggcgccttc ctctcggcgg
     1141 acatgaaggg ccccaagccc atcgactgca agctattcaa gctgtccaac gtcggcctgg
     1201 agatcggcgt cgactgggag ggcatcccca gcctgggcgt caccgccacc atcgacgtca
     1261 agagcttcga gtcctcggtg gccgtgttct tcgactcggc agatccgagc aagagcctga
     1321 tcgcgggcgc ggtgagcgat ctcacgctga aggaggtagt cgacacgctc ctcggcggtc
     1381 cggtcaagtc ctcgatcgac ggcgtcctgg catcgatcgc cgtccgcggc acccagcagt
     1441 tctcgatccc ggccaccctg gccgacgacc tcgcgcagct gcgtctggac tcgatcgcag
     1501 ccgccttcca gagcgccggc aagatcaaga tccccgccag cgcgtcgcag gtcctgctga
     1561 gcgccaacgt cccgggaaag gtctggcatc tgaccgacct gaccaccatg cgccactacc
     1621 agctcacgaa gcaggatacg tcgatcgtgg tgtccctcga ggcgcagttc tacttcgcgc
     1681 cgcaagccac ctcgataggc accatcgtct tcccgcaggg cttcttcatc aacggcgccc
     1741 tcaccttcct gggcttccat gccgaggcaa cggtcaacat cagcgcgggc aaaggcatca
     1801 gcgcggacgc gcatatggac aagatcgaga tcggaaaggg gggtgtattc gctctgacct
     1861 cggcggatgg caagcgcgga ccggagctct cgatctcgac gatgacgcag cccaacaacc
     1921 cggtcgccga gttccgcacg ccgcacatgt acatcagcgg cgcggcgcag ctgctcggga
     1981 tccggaatca ggtgctggcc cgcttcagca cgcagggcat gctgctggct ctgaaaggcg
     2041 ccctggctcc gggcgccgag ttcgacctcg acatccagtg cgcagcctcg ggcgtcaccg
     2101 tcgatggtga cgtcaaggcc gggatcggta cgatcgacct gggccagctc ggcaaggtca
     2161 aggtcaacac cgacgtcgaa ggcaccctgg gtgtacgcgc cagcgcccag caggtcgccg
     2221 tcgccgccga agccagcttc agcttcctcg gcgagaacaa ggagatcgcc aagttcgcgc
     2281 tggacaccaa gcccgacgcg ctggtccact tcgccgatac gctgaagagc aaggcggagg
     2341 ccctgatccg agaggagttc aaggactcca cgcgctgggc caacgccgtc aagagcggcg
     2401 cggtcgaggg cgtcaccgac accgagaagg tcctcaagaa cgtctacggc aagagcgaca
     2461 aggaggccaa ggcggtggcg tcggacatcg ccggcggctc caaggcggcg gcgaagacgg
     2521 tgaccaagtc cgcgagcaaa ttcggcaaga agctcaagaa ggtattctga ccgtgtcgat
     2581 tctcgaagca gctgccgtcg tgttcaacag ccttcgcgat ccgtgatcga tgactttggc
     2641 gcgtcgtgtg ccaggcgacc tcaggtacat cgcagctcgc gctgatccga agcctgatca
     2701 acgggtcttg ggtgaatccg cgccggttgc gcgttcgtcg cccagcacga cccgagcgcc
     2761 ggagccgtcg aatcgcagcg tcttcgcctt gccgcgattc ggattgcgca cgatcaccga
     2821 gtagaccgcc tgcacctcga accgaccggg atagccggcc gaggtgtgta cgatcttcac
     2881 ggtgcccgcg gccttgtcga tctccgggtg ggccttgagc accggctcga agaagctggc
     2941 tcgtgtgtcc ggtgaaccga gccggcgcga gagctcctgt gcgcgcgcca cgacgtcggc
     3001 tgggatcgac ggccagtaca cgccctcggt gatcgccgcg ccgctcgtat cgagcgctgc
     3061 gaaggcaaac gaaccttcga cgggaacccc accgaccgag cgctcgaggt gcgtgctgta
     3121 ccagagcagc cgctgcgacc acggcgcgag cttgccgtcg cgcgacatgc cgccggccat
     3181 ggtggtggtc acgtgcgtgc cgctgacctc ggaggtcggc acaccggatc gaaccagata
     3241 gtcactcgca acacggctgt gctcctcggc gctctgggac agtggttctg gatactggtc
     3301 cggacgcatg gcggtacgga cggcaagcac tgctccggtg actcgatcgg tggcgaacga
     3361 gccgcgcgcg ccggtgaccc gcacgaccgg tccatcggtc tcctcatgtt cgccctcgcc
     3421 aacgtcgaat cgcgattgtg cgaggcggtg ctggtaggcc agaccctcgg tggcggcggc
     3481 gaggtcgccc gccggagcat cggacctggg tgagcagccg ccgaccacga ggagcaagac
     3541 aaggggaagc gatttcgttg ccatcagagt cctcatgtca cacctcaggg aagaacgaag
     3601 gggttggcgt tgcagtccta gttgcaggtc cagatggccc acatgaagtt atggccggtg
     3661 gagtcattgc cctcgttgcg gaagccggcc gagttctcgg acagcgtgct gaactgcgcc
     3721 cgccagagcg tggagtcaac ggagatggcg atgtaggcgc cgatgatctg tgccgatgat
     3781 gagtcacgta cgctcgttca aatcgctgac actggcacac tgtgccaggt gtgcctgcgg
     3841 tcgatcgcgt cggcattgga cagcgccagc tccttgaccg ggatgctgcc ctggtacgtg
     3901 agcgcgcgga tcgtcgcgac cagcaccagg gcgtcgggcc acagccacga cgcacggcac
     3961 ttgaggtcgc ggagcttctc gccgccgagg tagaagccga accctgccac cggcgcaggc
     4021 cgcggcagcc gctgtgccgt caggtgtcgg cgtctgccgg tgacccaccg agcggcacgc
     4081 tgaatgggcg cgccgatcct gcgcgctgct ttccacgcgg gagctaagtc tgcattctcc
     4141 gcgatggcgg tcgaggaacg tgcgttgcag gcgacctcgc ggaggctagc aatgatgaga
     4201 ctcttcttca tgacgacgct gctcgcggcg tgcgcgaacg cgtccgggtc cgcaaccgcc
     4261 gagggtgcgc tgtcggcatc gatcgtgtgc ctggcgccga tcggtggctg gaacgtgaag
     4321 ctccagggca gcacgtggaa cgacgaggcg gatgcgatcg ccgcggacaa cagcgattgc
     4381 gcgatctacc ttgccggtga cacggtcaac gtgctcccgg gcgccacgtc gttcggcggc
     4441 caggatgcgt tcctgatccg gtacaattcc agtggatccg tggtgtggac gcggcagttc
     4501 ggcagcttgc tgggcagcga cgaggcggag tgggcgaccg gcgtcgcggt ctcggccaac
     4561 cacgagatct atgtcaccgg gtgcaccacc ggccagatga cgaatgcacc ggagaccaac
     4621 gcgggaggca cggacgtctt cctcgccaag tacgatcacg atggcaacca gctgtggatc
     4681 caccagttcg gcaccaccac cgatgactgc agcacggggg tcgccgtcgg ggtcagcggc
     4741 ggcgtgtttg tcgtcggcag cacgcacgcg ttcctgcccg gatcgctgtc ggcctacttc
     4801 ggcggggcgg atgcattcct cgtcaagtac agcagcgccg gcgtacggca gttcacccag
     4861 atgctcggct cgccggtgga cgacgaggcc agcgccgtcg cgatcggccc gagcggcaat
     4921 gtctatgtca ccggtgatac cgaggaggat ctgccgcgct tcgccaactt ccccgacgag
     4981 cagggcacac gcaacggcgg tgcggacatc ttcctcgcca agtacgatcc gacgaccggg
     5041 gccccgatct ggttgcgcca gcgcgggacg agcggggaag atcgcgccaa cggtgtcgtg
     5101 gtcaacgact cgaacgaggt gttcttgacc ggctacacga atggctggtt cgatggcatc
     5161 ccgacccacg gcctcgagga catcgtcgtg atcagctacg ataccgccgg cacgtggcgg
     5221 tggaccgacc agcgcggctc gctcggcgac gaccgcggca cggcgatcac ggtcgactcc
     5281 agcgggacgc cgttcgtcac gggctacgcg agctcgacgg tcgacggcga gccctacgcc
     5341 ggtggcacgt ccgacgtggt cgtcatcaag tacggccgcg caggcgtcca ccgctggacc
     5401 gcggatctcg gcgagcgcga caacgagcgg ccgacgggca tcgccgccgg cgtgggagac
     5461 gacctcttcg tgggcggctt caccgacggc aacctcggca acgcgctcgg gttcggtgag
     5521 aacgacgctt tcgttgcccg gttctcgcag accggcgatc tggactgatc cgccgccgcg
     5581 atcaggtcag gccggtgacc tcgccggcgt cgtcgagatc gatgtgctcg gcggccggcg
     5641 cgtcgggcag gccgggcatc cgcatgatgt cgccgcagat cgggatcagg aacccggcgc
     5701 cggcggcgat ctcgatctcg cgcacggcga gcgtgaagcc ctcggggcgg ccgagcaggt
     5761 tggcctgctc ggagaacgag tactgggtct tggccatgca gatcggcagg ttcgtcagcc
     5821 cgagctcggc gatggtcttg aggtcggtcc tggccttggc cgcgagcgcg atctcgccgg
     5881 cgccgtacag gccgcgcgcg atcgcctcga tcttggcctc gaccggggcg tcgagcgggt
     5941 aggcgaactt cggcgcgcgc gtgccgctct cggccgcggc gacgaccgcg gccgccgcgt
     6001 ccatcgcgcc ctcgccgccg tgcgcgaagt gatcgctcgc gaccacggtc ccctcgtgct
     6061 tggcgcacag ctcgcggacc agggcgagct cgtcgtcgtg atcggctgcg aaccggttga
     6121 tgacgaccac cggccgcagg ccgaagaacc gggcgttgtc gaggtgccgc gcgaggttgg
     6181 ggaagccgcg gcggatcgcg tcggcattgg gcaccgccag ctccttgacc gggatgccgc
     6241 cctggtactt gagcgcccgg atcgtcgcga ccagcaccag cgcgtccggc cacagccccg
     6301 acgcgcggca cttgaggtcg aggaacttct cgccgccgag gtcgaagccg aacccggcct
     6361 cggtgaccac ccagtccgcc aggctcaagc ccaggcgggt cgcgaccacc gagttggtgc
     6421 cctgggcgat gttgccgaac ggtccaccgt gcaggatcgc cggcgtgccc tcgagggtct
     6481 gcaccaggtt gggctggatc gcctcgcgca gcagcgcggt catcgcgccg tggaccttga
     6541 gatcgcgggc gtacaccggc cgccggccgc ggctgaaccc gatgaagatg ttgccgagcc
     6601 gctccttcag atcggcggtc gactgcgcga agcacaggat cgccatcacc tcggaggcgg
     6661 cggtgatgtc gaacccggcc tcgcgcggca cgccgctgcc cgacccgccg agcccgatca
     6721 cgatccggcg cagcgcgcga tcgttcatgt cgatcacgcg cttccagcgg atggtcagcg
     6781 ggtcgagatc cagggtgctc ttcttgctgt gcaggtggtt gtcgaccacc gcggcgagca
     6841 ggttgtgggc cttctcgacc gctgcaaaat ctccattgaa gtgcagattg atatcggcgt
     6901 gcgggatcag gcgcgcgcgg ccgccgccgg tcgcgccgcc cttcatgccg aacaccggcc
     6961 cgagcgatgg ctcgcgcagc acggcgaccg cgcgcttgcc cagccgagac aggccatcgg
     7021 ccaggccgat cgacgtcgtg gtcttgccct cgccggccgg cgtcggcgtc accgccgata
     7081 ccaggatcag cttcgatttg gcggcgcgcg ccagatcgat ccgcgcgagc ggcagcttgg
     7141 ccttgatgcg tccatacggc tcgatctcgt cggcggtcag gccgagccgc gccgcgatct
     7201 cgacgatcgg gcgcggcttc gcggtcgcca gcgggtcggc aacagcctcg gaatgaggtg
     7261 gaatcatccg ggattcatcc catcccggcc ggtccggatg caaccgccac gcgatgacta
     7321 catcggacgc aacacatttc gcgacgcgac gcgacgcggg aagtgacccg tcgcgtcatg
     7381 ctgcaacgcg aggcgcacac gctacgatgg ccagacaggc ccggacacga ccggcgaggg
     7441 agcggacgat gcacaggaag acgatgttcg gattcagcgc ggtggccagc gcggtgtatc
     7501 gcgtgaccac cgagtcgaat gtctacatcc tcggctttca cgaggagcgc ggccgcaagt
     7561 acgtggtggt gcgcggcctg cccggcaccg accgcgagca cgtggtgctg cgcgacagcg
     7621 atccgcggat cggcgaggtg tcgatgttcg aggtgccgcc gcaccagtgg atcggcaaga
     7681 acatggagat cgcgacgatg tcgtccagcg aggtcaccgc ggtgacgctc gagaccgacc
     7741 gcgcggcgat ctcgtcggtg ggcggcgact cgccgtcggc gcgcagcgcg tggagtccgg
     7801 cgaccgggga gaccatgccg ggatttccgt cgcggccgcc ggggctcccg gagctgcccg
     7861 gcgtccgggc cgggctgg
//
LOCUS       DASVOR010000141         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_89024,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000141 DASVOR010000000
VERSION     DASVOR010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89024"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /gene="nth"
                     /locus_tag="VF516_02920"
     CDS             complement(<1..829)
                     /gene="nth"
                     /locus_tag="VF516_02920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX8106648.1"
                     /translation="MGSRTRNSTPKPLRGAPITPDLGAPDLGADLAAPRARTAAIADR
                     LAELWPDAAVELDHDSAYQLLVATIHAAQSTDKLINTVTPRLFARYPDPAALARADQA
                     ELESMIFSTGFFRMKARHLIGMAQAIVERHGGQVPDTMAELTGLPGVARKTANVVLGS
                     ALGKNEGIVVDTHVTRLAQRLGLSAHTDPVKIEQDLMQLVPRDQWSIFAHRLIWHGRR
                     VCHAKQPDCNHCALAPLCPSAFLVDTPHLMPPRNGRPALPSRAQRPATAKQRASQLAK
                     "
     gene            828..1754
                     /locus_tag="VF516_02925"
     CDS             828..1754
                     /locus_tag="VF516_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106649.1"
                     /translation="MQRHRITFLAAIICATGCTTHKTEVETAKKSLYDAEFTVVYNAA
                     LEATRETYPNIEDAPGRGAIKTAWHQVSYANNQDDMTNPRTLANAQGINPGAAGTPGA
                     ASAGMPTRLAYKRYFIRFDISVAGGRPWRLKVVGHAAEWDPGNALPTELHGPARPSWL
                     DGRTDSLVYAIYERIKKYAVPMPDNQAASDEIKHADPAAFGAVPPDAAKELASIKDVL
                     DARDYAGLRPHLADDVVWSLGGGSGPDGAMALWQADPEPLDAMAKALAAGCAGDTKKV
                     TCPGGAPAAGSYQLSLEPRGAAWRVTSYVKNE"
     gene            1901..3766
                     /locus_tag="VF516_02930"
     CDS             1901..3766
                     /locus_tag="VF516_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106650.1"
                     /translation="MRWSYLVLYLAGCGFHSSAGAPTPDAPLPIPDGGVREMRSLGSN
                     EFKAGQLVDMTLDAVRGSLTPNAYTYGGLIAHGLAGMKLWDHDHTTWMTPATANATVS
                     GLWRGDSFATADDLTYVGVSNDQTMTVWFEGEVWLDATSTETFGLTADDVAFIDLARP
                     GMTSFLRVMQNDVATAAVATPDTGWYPIRIGFANGDGTLGFGFTHSDSAAGPQVAWTR
                     DRLRARGSEVSGALRTVFAHQILGGGGPSKPPISHVDDGTLLQNTTFPNPPLQGASTD
                     NNSWSARYAAQVYVAQMGSYTLKVTSDDGNEMWFGGQAKNASWGFGSGNSNSMSTVTA
                     NLDVGWNDAIVDYNQVGGGRKLQVTLMGGGLNADIPRAMLRPVEPALDRLAFGSDDTS
                     HLVQDNGGPALPGTAILNVAAYGGSTAETVTSIDVTYEVNSPHWNELRIDLESPTKRI
                     NIANGGGVANGDSIDQTSIAAGTGGPLGMLLGGPAGGTWKLHVYDTQDSGASGDSTLK
                     SAKLTLHTTGGPDKIARTASWTSQVIDATSNVIAIDSLTWQARVPAGAGVQVRLRGCQ
                     QSDCSDGPAWSGPVTSGMPFAITPARYLQLQVAMTGDGSHEPELQSLALVFRRSM"
     gene            complement(3789..>4418)
                     /locus_tag="VF516_02935"
     CDS             complement(3789..>4418)
                     /locus_tag="VF516_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106651.1"
                     /translation="WRVIHGAAELLGELVEPPVDSVVFCSILHEIYSYTEPRFSLASV
                     QRVIHAAFSALAPGGRIVIRDGVQPPPGRRRLRLIAPDAPATFDLYVAQFEGRRIHVT
                     ELGGDRVELSTADAMEFLYTYTWGPASFPYEVRELYGILPYDDYVAQLVAWCGGPRAV
                     RVVELPPEIRSYLQPGYREHLAGRIALTDERDRPVDLPDSNCLIVLEKL"
BASE COUNT          696 a         1489 c         1619 g          614 t
ORIGIN      
        1 ccttggcaag ctggctggcg cgctgcttgg cggtcgcggg gcgctgggcg cgcgacggca
       61 gggccgggcg gccgttgcgc ggcggcatga ggtggggggt atcgaccagg aaggcgctgg
      121 ggcacagcgg cgccagggcg cagtggttgc agtcgggctg cttggcatgg cacacgcgcc
      181 ggccatgcca gatcagccgg tgtgcgaaga tcgaccactg atcgcgcggc accagctgca
      241 tcagatcttg ctcgatcttg accgggtcgg tgtgcgccga caggccgagc cgctgcgcca
      301 gccgggtgac gtgggtgtcg accacgatgc cctcgttctt gccgagcgca cttccgagca
      361 cgacgttggc ggtcttgcgc gccacgccgg gcagtccggt cagctcggcc atcgtgtcgg
      421 gcacctggcc gccgtggcgc tcgacgatcg cctgcgccat gccgatcagg tggcgggcct
      481 tcatccggaa gaatccggtc gagaagatca tcgactccag ctcggcctgg tcggcgcgcg
      541 ccagcgcggc ggggtcgggg tacctcgcga acagccgggg ggtcacggtg ttgatcagct
      601 tgtcggtcga ctgcgcggcg tggatcgtcg cgaccagcag ctggtaggcg ctgtcgtggt
      661 cgagctcgac cgccgcgtcc ggccacagct cggcgaggcg gtccgcgatc gccgcggtcc
      721 gcgcgcgcgg tgcggcgaga tcggcgccga gatcgggggc gccgagatcg ggggtgattg
      781 gggcacctcg caggggcttc ggtgtgctat tccgagtccg ggatcccatg cagcggcacc
      841 gtatcacctt cctcgcggcg atcatctgcg caaccggctg cacgacccac aagaccgagg
      901 tcgagaccgc gaagaaatcg ctctacgacg ccgagttcac cgtggtgtac aacgcggcgc
      961 tggaggcgac gcgcgagacc tatccgaaca tcgaggatgc gccggggcgc ggtgcgatca
     1021 agaccgcgtg gcatcaggtg tcgtacgcca acaaccagga cgacatgacc aacccccgga
     1081 cgctcgccaa cgcgcagggg atcaaccccg gggcggcggg gacgccggga gcggcgtcgg
     1141 cgggcatgcc gacccggctg gcgtacaagc gctacttcat ccggttcgac atctcggtcg
     1201 cgggcggccg gccgtggcgg ctcaaggtcg tgggacacgc cgccgagtgg gaccccggca
     1261 atgcgctgcc gaccgagctg catggtccgg cgcgaccgtc gtggctggac ggccgcaccg
     1321 attcgctggt ctatgcgatc tacgagcgga tcaagaagta cgcggtgccg atgccggaca
     1381 accaggccgc ctccgacgag atcaagcacg ccgatccggc ggcgttcggg gcggtgccgc
     1441 cggacgccgc caaggagctc gcctcgatca aggacgtgct cgacgcgcgc gactatgcgg
     1501 ggctgcggcc gcacctcgcc gatgacgtcg tgtggtcgct cggcggcgga tcgggccccg
     1561 acggcgcgat ggcgctgtgg caggccgatc ccgagccgct cgacgcgatg gccaaggcgc
     1621 tggccgcggg ctgcgccggt gatacgaaga aggtgacgtg ccccggcggg gcgccggcgg
     1681 ccggcagcta ccagctcagc ctggagccgc gcggcgcggc ctggcgggtc acgtcgtacg
     1741 tcaagaacga gtgaccgcct ggcgcgaaac gctgcaccgg tcccgggacg tctctttttg
     1801 acgttttgtg tgaccttttg gagtgtgact cttatcgcga acagtccgtc gtcatgccga
     1861 gaattgtcgg atgcgggcgg actctcggta ggattctgaa atgcggtgga gctacctcgt
     1921 cctgtacctg gcgggttgtg ggtttcattc gtccgccggt gcccccacac ccgatgcgcc
     1981 gctgccgatt cccgacggcg gtgtccggga gatgcggagc ctcgggtcga acgagttcaa
     2041 ggcggggcaa ctcgtcgaca tgacgctcga tgcggtgcgc ggctcgctga cgcccaacgc
     2101 gtacacctac ggcgggctga tcgcgcacgg cctggcgggc atgaagctgt gggaccacga
     2161 ccacacgacc tggatgaccc ccgcgaccgc gaacgcgacg gtctccgggc tgtggcgcgg
     2221 cgacagcttc gcgacggccg atgacctgac gtacgtcggg gtgtccaacg atcagacgat
     2281 gacggtgtgg ttcgagggcg aggtctggct cgacgccacg tcgaccgaga cgttcggcct
     2341 gaccgccgac gacgtcgcgt tcatcgacct cgcgcgcccc ggcatgacga gcttcctgcg
     2401 cgtgatgcag aacgatgtcg ccacggcagc ggtcgcgacg cccgacaccg gctggtatcc
     2461 gatccggatc gggttcgcca acggcgacgg cacgctcggc ttcgggttca cgcacagcga
     2521 cagcgcggcg ggcccgcagg tcgcgtggac gcgcgatcgg ttgcgcgcgc ggggcagcga
     2581 ggtgagcggc gcgctgcgca cggtgttcgc ccaccagatc ctcggcggcg gcggtcccag
     2641 caagccgccg atctcgcacg tcgacgacgg cacgctgctg cagaacacga cgttcccgaa
     2701 tccgccgctg cagggtgcct cgaccgacaa caacagctgg tcggcgcgct acgccgccca
     2761 ggtctacgtc gcgcagatgg gcagctacac gctcaaggtg accagtgacg atggcaacga
     2821 gatgtggttc ggcggtcagg ccaagaacgc cagctggggc ttcggcagtg gcaacagcaa
     2881 ctccatgtcg acggtgaccg ccaacctgga tgtcggctgg aacgacgcga tcgtcgacta
     2941 caaccaggtc ggcggcggcc gcaagctgca ggtcacgctc atgggcggcg ggctcaacgc
     3001 cgacatcccg cgcgccatgc tgcggccggt cgagcccgcg ctggatcggc tggcgttcgg
     3061 cagcgacgac acctcgcacc tggtccagga caacggtggg ccggccctgc ccgggaccgc
     3121 gatcctgaac gtcgccgcgt acggcggcag cacggcggag acggtgacgt cgatcgacgt
     3181 cacctacgag gtgaactcgc cgcactggaa cgagctccgg atcgatctcg agagcccgac
     3241 caagcggatc aacatcgcca acggcggcgg ggtcgccaac ggggattcga tcgaccagac
     3301 ctcgatcgcg gcgggcaccg gcggaccgct cggcatgctg ctgggcggac ccgccggcgg
     3361 aacgtggaag ctgcacgtct acgacaccca ggacagcggc gcctcgggcg acagcacgct
     3421 caagagcgcc aagctgacgc tgcacaccac cggcggaccc gacaagatcg cgcgcaccgc
     3481 gtcgtggacg tcgcaggtga tcgacgcgac gagcaacgtg atcgcgatcg acagcctgac
     3541 ctggcaggcg cgcgtccccg ccggcgccgg cgtccaggtc cggctgcgcg gctgccagca
     3601 gagcgactgc agcgacggcc ccgcgtggtc ggggccggtg accagcggca tgccgttcgc
     3661 gatcactccc gcgcgctacc tgcagctcca ggtcgcgatg accggcgacg gcagccacga
     3721 gcccgagctg cagtcgctgg cgctcgtgtt ccgccgcagc atgtgacgcc ggcggatcgg
     3781 cgggccggtc acagcttctc gaggacgatc aggcagttcg aatccggcag gtcgaccggg
     3841 cgatcgcgct cgtcggtcag cgcgatcctg ccggcgaggt gctcgcggta gccgggctgg
     3901 aggtacgagc ggatctccgg cggcagctcg accacgcgca ccgcgcgcgg cccgccgcac
     3961 cacgcgacga gctgggcgac gtagtcgtcg tagggcagga tgccgtagag ctcgcggacc
     4021 tcgtagggga agctcgcggg cccccaggtg tacgtgtaga ggaactccat cgcgtcggcg
     4081 gtcgacagct cgacgcgatc gccgccgagc tcggtgacgt ggatccgccg gccctcgaac
     4141 tgcgcgacgt agaggtcgaa cgtggcgggc gcgtcgggcg cgatcagccg gagccggcgg
     4201 cgtccgggcg gcggctgcac accgtcgcgg atcacgatcc ggccgccggg cgcgagcgcc
     4261 gagaacgccg cgtggatcac gcgctggacc gacgccagcg agaaccgcgg ctcggtgtac
     4321 gagtagatct cgtgcaggat cgagcagaac acgacgctgt cgaccggcgg ctcgaccagc
     4381 tcgccgagca gctccgccgc gccgtggatc acgcgcca
//
LOCUS       DASVOR010000142         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_59752,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000142 DASVOR010000000
VERSION     DASVOR010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59752"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="VF516_02940"
     CDS             <1..881
                     /locus_tag="VF516_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX8106652.1"
                     /translation="RRGASERARLTIEPAAHPFTHLAIENPLGDVKVEGYDGTAIQIE
                     SHKFAPDDEALDRLHISLVPNPDGTVSIKTTADGGKEVHPVLRRQVRIDLVIHAPRNA
                     RIEAAASSGALEVINMDAGSDLDTASGPISVRNVQGEVLTHSVSGLTRLVQVFGSVDA
                     QTLASDLDLDTIGGERVIASVHRGRIDGRRVRARDVELTATEGKILLEAEAALQGRLL
                     VSAFKGDVDVQLRRHGAVMVRAQGTKVDLGGPMLPRPNGWVESSFGQATTGDAAVVEI
                     RSPQGNVRFAIIESGP"
     gene            915..1586
                     /locus_tag="VF516_02945"
     CDS             915..1586
                     /locus_tag="VF516_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106653.1"
                     /translation="MLDSVPPRVREYADQAVSYVRRALGIQLEYDSNTLPVLDHYLRT
                     VPEDQPAALQLVVATSGAYFGEVVRQRLGGRWELADQEAEWRIVLPTGLNFSPVGFVA
                     AAIARADLEDLDSEIAAPPRMLPYVQRALARMGEVTIEDYYSLCGRLDTLEHVHEVLV
                     AVAAQMLGDDDLDEAAAGEPDGAPDADAEDDDEDTDDAADDDDPSDDAEPELVADDEI
                     SGPVN"
     gene            1616..>1912
                     /locus_tag="VF516_02950"
     CDS             1616..>1912
                     /locus_tag="VF516_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106654.1"
                     /translation="MTGPPYLGEIGKTRTSTVHVIDSRGALVAVEVYTGIDAVANPAL
                     AGQLRDDSLNVVQLGGGEMRIEVPVVYHDPAARLMVLVLGDAHRHREIEERIRLL"
BASE COUNT          283 a          685 c          690 g          254 t
ORIGIN      
        1 cgcgtcgcgg cgccagcgag cgcgcccggc tgacgatcga gcccgccgcc cacccgttca
       61 cccacctggc gatcgagaac ccgctgggcg acgtcaaggt cgagggctac gacggcacgg
      121 cgatccagat cgagagccac aagttcgcgc ccgacgacga ggcgctcgat cgcctgcaca
      181 tctcgctggt gcccaacccc gacggcacgg tctcgatcaa gaccaccgcg gacggcggca
      241 aggaggtcca cccggtgctg cgccgccagg tccggatcga cctggtgatc cacgcgccgc
      301 gcaatgcgcg gatcgaggcc gcggcgtcga gcggagcgct cgaggtcatc aacatggacg
      361 ccggcagcga tctcgacacc gcgtcgggcc cgatctcggt gcgcaacgtc cagggcgagg
      421 tgctgaccca cagcgtgtcg ggcctgaccc ggctggtcca ggtgttcggt tcggtcgacg
      481 cccagaccct ggcgtcggac ctcgacctcg acacgatcgg cggcgagcgg gtgatcgcct
      541 cggttcatcg cggccggatc gatggccgcc gggtccgcgc ccgcgacgtc gagctgaccg
      601 cgaccgaggg caagatcctg ctcgaggccg aggccgcgct acaggggcgc ctgctggtgt
      661 ccgcgttcaa gggcgacgtc gacgtccagc tccgccgcca cggcgccgtc atggtccggg
      721 cgcagggcac caaggtcgac ctcggcggcc cgatgctgcc ccggccgaac ggttgggtcg
      781 agtcgtcgtt cggccaggcc accaccggcg acgccgccgt cgtcgagatc cgctcgcccc
      841 agggcaacgt ccggttcgcg atcatcgaga gcgggccgta gcccggtccc gcgaaccgcg
      901 gtaggctggc cgacatgctc gattcggttc ccccgcgcgt tcgcgagtac gccgatcagg
      961 cggtctcgta cgtccggcgg gccctcggga tccagctgga gtacgacagc aatacgctgc
     1021 cggtgctcga ccactacctg cgcaccgtcc ccgaggacca gcccgccgcg ctccagctcg
     1081 tggtcgcgac ctcgggtgcg tacttcggcg aggtcgtccg ccagcggctc ggcgggcgct
     1141 gggagctcgc cgatcaggag gcggagtggc gcatcgtgct gccgaccggc ctcaacttct
     1201 cgccggtcgg gttcgtcgcc gcggcgatcg cgcgcgcgga tctcgaggat ctcgactccg
     1261 agatcgccgc gccgccgcgc atgctgccct acgtccagcg cgcgctggcg cggatgggcg
     1321 aggtgacgat cgaggactac tactcgctgt gcgggcggct cgacaccctg gagcacgtcc
     1381 acgaggtgct ggtcgcggtg gcggcacaga tgctcggcga cgacgatctc gacgaggccg
     1441 ccgcgggcga gcccgatggc gcgccggacg cggacgccga ggacgacgac gaggacaccg
     1501 acgacgccgc cgacgacgac gacccgagcg acgatgccga gccggagttg gtcgccgacg
     1561 atgaaatcag cggtccggtc aattagccga tggcgcgcag gccggggagc tgcaggtgac
     1621 cggtccgccg tacctcggcg agatcggcaa gacccggacg tccacggtcc acgtcatcga
     1681 ctcgcgcggc gcgctggtcg cggtcgaggt ctacaccggg atcgacgccg tcgcgaatcc
     1741 ggcgctcgcc gggcagctgc gcgatgacag cctgaacgtg gtgcagctcg gcggcggcga
     1801 gatgcggatc gaggtgccgg tggtctacca cgacccggcc gcccgcctga tggtgctggt
     1861 gctgggcgac gcgcatcgcc accgcgagat cgaggagcgg atccgcctgc tc
//
LOCUS       DASVOR010000143         2893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439621,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000143 DASVOR010000000
VERSION     DASVOR010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439621"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VF516_02955"
     CDS             <1..571
                     /locus_tag="VF516_02955"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8106655.1"
                     /translation="VPPPGLDHDLAAARREIALARAEGGVPDRPIEYRYNAGSEVHQQ
                     IAEYLQAAWAQIGVPVVLIAQEWNSLLDATARGEFEIARFGNVGTAIDTESEFLALLR
                     CGSPFNRGRYCSPVFERRMSEAHGMVDRRARNAVLREAEAVMLEDAPLIPLYVLTQKH
                     LIKPYVRDYAVNLLDLPPLWRIWIDPGAR"
     gene            568..1494
                     /locus_tag="VF516_02960"
     CDS             568..1494
                     /locus_tag="VF516_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8106656.1"
                     /translation="MIRFVAGRLAASVAVVFAVATASFLLLHAAPGGPFDTDQRRSPA
                     VQRAMEDRYGLHEPLWRQYGRAMAQLARGDLGVSMKRDVSVSRLIAEHGPISALLGLV
                     ALVSALAIGVALGAIAAWRRNTWVDALVMTGALIGISIPAFVLGPLLIAAFSLHLGWL
                     PPARIAGARSLILPAITLGGVYLAAIARLTRGGLLDVLDQDFVRTARAKGLAEGRVIA
                     RHALRLGIAPVVTYLGPAVAALISGSFVVEKIFQIPGLGTYFITSVAERDYPVVTGVF
                     VFYAALLAVCNLAVDLAYGALDPRLREGERAR"
     gene            1491..2384
                     /locus_tag="VF516_02965"
     CDS             1491..2384
                     /locus_tag="VF516_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8106657.1"
                     /translation="MTAPGASAWQRFRANRMAVVCGGVFLVIAAACYAGPLIAGAAGL
                     DATTIDVRLGATPPGAAHWLGTDTLGRDMLVRVMVGGRIAIAVALVAAAIAVVIGVSY
                     GAIAAYAGGAIDNAMMRLVDALYGFPTLVLVIVVRAVTGTRSLFALFALIGAISWLTL
                     ARITRGQVLALRRREFVEAARALGAPPARILVRHVLPNAAGPVIVYATLALPQVMLTE
                     ALLSFLGLGVQAPLASWGTLVTEGSSQIVVYPWLLVFPAIAMGTAILALNFIGDGIRD
                     AVDPGRAAPPRARPRGQTGSR"
     gene            complement(2366..>2893)
                     /locus_tag="VF516_02970"
     CDS             complement(2366..>2893)
                     /locus_tag="VF516_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106658.1"
                     /translation="PIGLGHGPVSLAPPVWDAYRALEADPGEPAVHRLLAALAGSGVV
                     SPDLAASVVAEEPERFARVWAVMQPLYQHLATSASLKQIASLAGLSLRQLGRDLADLT
                     RTFGLFGGGFRDAMRILRLRAAVLLLSAPHATPNDVARVVGYSSIDAMGRAFRDARLP
                     PPSLVHSAVRYRDPV"
BASE COUNT          334 a         1030 c         1114 g          415 t
ORIGIN      
        1 cgtgccgccg cccgggctcg accatgacct ggcggcggcg cgccgcgaga tcgcgctggc
       61 ccgcgccgag ggcggggtgc cggaccgccc gatcgagtac cgctacaacg ccggcagcga
      121 ggtccaccag cagatcgccg agtacctgca ggcggcctgg gcgcagatcg gcgtgccggt
      181 ggtgctgatc gcgcaggagt ggaactcgct gctcgacgcc acggcgcgcg gcgagttcga
      241 gatcgcgcgg ttcggcaacg tcggcaccgc gatcgacacc gagagcgagt tcctggcgct
      301 gctgcgctgc ggctcgccgt tcaaccgcgg ccggtactgc tcgccggtgt tcgagcgccg
      361 gatgagcgag gcccacggca tggtcgaccg gcgggcgcgc aatgccgtgc tgcgcgaggc
      421 ggaggcggtg atgctcgagg acgcgccgct gatcccgctg tacgtgctga cccagaagca
      481 cctgatcaag ccgtacgtcc gcgactacgc ggtcaacctg ctcgacctgc cgccgctgtg
      541 gcggatctgg atcgacccgg gagcgcggtg atccggttcg tcgccgggcg gctggccgcg
      601 agcgtcgcgg tcgtgttcgc ggtcgcgacc gcgagcttcc tcttgctcca cgccgcgccg
      661 ggcggaccgt tcgacaccga ccagcgccgg tcgcccgcgg tgcagcgcgc gatggaggac
      721 cgctacgggc tgcacgagcc gctgtggcgg cagtacggcc gggcgatggc gcagctcgcg
      781 cgcggcgacc tcggggtgtc gatgaagcgc gacgtctcgg tgagccggct gatcgccgag
      841 cacgggccga tcagcgcgct gctcgggctc gtcgcgctgg tctcggcgct ggcgatcggc
      901 gtcgcgctcg gggcgatcgc ggcgtggcgg cgcaacacct gggtcgacgc gctggtgatg
      961 accggcgcgc tgatcgggat ctcgatcccg gcgttcgtgc tcgggccgct gctgatcgcg
     1021 gcgttctcgc tccacctcgg ctggctgccg ccggcccgga tcgccggcgc gcggtcgctg
     1081 atcctgccgg cgatcacgct cggcggggtc tacctggcgg cgatcgcgcg gctgacccgc
     1141 ggcggcctgc tcgacgtgct cgaccaggac ttcgtgcgca ccgcgcgcgc caaggggctg
     1201 gccgaggggc gggtgatcgc tcgccacgcg ctgcgcctcg gcatcgcgcc ggtggtgacc
     1261 tacctgggcc cggcggtcgc cgcactgatc tcggggtcgt tcgtcgtcga gaagatcttc
     1321 cagatcccgg ggctcgggac ctacttcatc accagcgtcg ccgagcgcga ctacccggtg
     1381 gtgaccggcg tgttcgtgtt ctacgccgcg ctgctcgcgg tgtgtaacct cgcggtcgac
     1441 ctggcgtacg gcgcgctgga tccgcggctg cgcgaggggg agcgcgcgcg atgacggcgc
     1501 ccggggcatc ggcctggcag cggttccgcg ccaaccggat ggcggtggtg tgcggcggcg
     1561 tgttcctggt gatcgccgcc gcgtgctacg ccggcccgct gatcgccggc gcggcggggc
     1621 tcgacgcgac gacgatcgac gtgcggctcg gcgccacgcc gccgggggcc gcgcactggc
     1681 tgggcaccga cacgctgggc cgcgacatgc tggtccgggt gatggtcggc ggccggatcg
     1741 ccatcgccgt cgcgctggtc gccgccgcga tcgcggtcgt gatcggcgtc agctacggag
     1801 cgatcgcggc gtacgccggc ggcgcgatcg acaacgcgat gatgcggctg gtcgacgcgc
     1861 tctacgggtt cccgaccctg gtgctggtga tcgtggtgag ggcggtgacc gggacgcgct
     1921 cgctgttcgc gctgttcgcg ctgatcgggg cgatctcgtg gctgaccctg gcacggatca
     1981 cccgcggcca ggtcctggcg ctgcgccgcc gcgagttcgt cgaggctgcc cgcgcgctcg
     2041 gcgcgccgcc cgcccgcatc ctcgtccgcc acgtgctgcc caacgcggcc gggccggtga
     2101 tcgtctacgc gacgctggcg ctgccccagg tgatgctcac cgaggcgctg ctgtcgttcc
     2161 tcgggctcgg cgtccaggcg ccgctggcgt cgtggggcac gctggtcacc gagggcagct
     2221 cgcagatcgt cgtgtacccg tggctgctgg tgttcccggc gatcgcgatg ggcaccgcga
     2281 tcctcgcgct caacttcatc ggcgacggca tccgcgatgc agtcgatcca ggacgcgctg
     2341 caccccctcg cgcgcggcct cgcggtcaaa ccggatcgcg gtagcgcacc gcgctgtgga
     2401 ccaggctggg cggcggcagc cgggcgtcgc ggaacgcgcg gcccatcgcg tcgatgctgc
     2461 tgtagccgac gacgcgggcg acgtcgttcg gcgtggcgtg gggggccgac agcagcagca
     2521 ccgcggcgcg cagccggagg atccgcatcg cgtcgcgaaa cccgccgccg aacaggccga
     2581 acgtgcgggt cagatccgcg aggtcgcggc cgagctggcg cagcgacagc ccggcgagcg
     2641 acgcgatctg cttgagcgag gccgaggtcg cgaggtgctg gtacagcggc tgcatcaccg
     2701 cccacacccg cgcgaatcgc tcgggctcct cggcgaccac gctcgcggcc agatcggggc
     2761 tcaccacgcc gctgccggcg agcgcggcga gcaggcggtg caccgcgggc tcgccggggt
     2821 cggcctccag cgcgcggtac gcgtcccata ccggcggcgc gagcgagacc gggccatggc
     2881 cgaggccgat cgg
//
LOCUS       DASVOR010000144         3593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_352071,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000144 DASVOR010000000
VERSION     DASVOR010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352071"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1933
                     /locus_tag="VF516_02975"
     CDS             <1..1933
                     /locus_tag="VF516_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106659.1"
                     /translation="VQGAVEQGAAMRIGEGFAGAIAARNEPMTLRHPTAAQLRSPAMA
                     RAGVRVLHGVPIVDRGEVIGVAKIGSLTAEQFSLQDQRIFAAMVARASAAIVQHMLRD
                     QARRASEQLAEQERQFRALADNIPQLTWMADAAGQRYWYNRRWYEYTGNTHEEMQGSG
                     WQKLHHPDHSQRVYQRWMRAVELGEPWEDIFPLRGADGIYRWFLSRAVPIRDASGAIE
                     RWFGTNTDVTVRRFLDNATEVLSRSLDERETLEQLARLAVPDIADWCVVDLVVQDQLE
                     YVAIAHDDPDKLEAAWQYARTHRSDPAGHDASWQIVRTGKPGVAAEISDEMLAAASRD
                     PDHLRLLHELGFRSWIGAPLVARGTAIGVIHLCMNDSGRRYREPDLEIAVELGRRAGV
                     AIDNARLYHEAQTAIRVRDDLLAIVSHDLNNPLHAIDLGATLLHQQFGTDARARKHID
                     AIRRSAERMERLINDLLDMASINAGKLAIKPAPFDARELVNEVLDSHQPFAHERGITI
                     TRSCDLDDVTLYVDRDRIAQAFGNVLGNAIKFCAPGDVITVLAERIGEGVRFTFADTG
                     PGIAPPDLPHIFEPYWSGRSGRKKGTGLGLFITRAILEAHGGTIQVDSPPGGGASFVV
                     TLPIGNIGIAPATTDPQTP"
     gene            1933..2814
                     /locus_tag="VF516_02980"
     CDS             1933..2814
                     /locus_tag="VF516_02980"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8106660.1"
                     /translation="MLNYNHLYYFHVAASESSLSAAAVKLGVKQSTVSEQLRTLERAL
                     RQRLFERSAGGMRLTAAGEVAYEHTTLMFRAGDRLLQALGRDEAPAPRSLRVGISGAV
                     ARATSTDFLLPLFALDDCMPTISTSETLDLLRDLRANELDLVLCETEPTEATVQGLRR
                     ALIEHSPLLAIAAADLDPGPDWQDTGLVQYRSSSTFHFEVKAFLEQRGLKPRIVGEAD
                     DPSLLVEAAARGRYVVIVPRSVVRDALAAGRVRILAQVESHAGVHALYQDGTAAELAR
                     RAIEKLIATARANVAAN"
     gene            3031..>3593
                     /locus_tag="VF516_02985"
     CDS             3031..>3593
                     /locus_tag="VF516_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106661.1"
                     /translation="MSWPTKGDAHTSPSLQPDAPGAAIEVLEADELLGIGLGSDILMV
                     DDDAASLAAYEAALSPLGRRLVAVQSGVAALARLLEQDFALVLLDVSMPGMTGLETAR
                     RIRERPRNRGLPIVFITGVAGSTAVMLEAYDAGAFDFIVKPVLPEVLRAKARVYLQLQ
                     ERTQALRRHSAELREAHAELARAGEQLR"
BASE COUNT          538 a         1307 c         1255 g          493 t
ORIGIN      
        1 cgtgcagggc gcggtcgagc aaggcgcggc gatgcggatc ggcgagggct tcgccggcgc
       61 gatcgccgcg cgcaacgagc cgatgacgct ccgccacccg accgcggcgc agctgcgcag
      121 cccggcgatg gcgcgggccg gggtccgcgt gctgcacggc gtgccgatcg tcgatcgcgg
      181 cgaggtgatc ggcgtggcga agatcggctc gctgaccgcc gagcagttct cgctccagga
      241 ccagcggatc ttcgcggcga tggtcgcgcg cgccagcgcg gcgatcgtcc agcacatgct
      301 gcgcgaccag gcgcggcggg cgtccgagca gctcgccgag caggagcgcc agttccgcgc
      361 gctcgccgac aacatcccgc agctgacctg gatggccgac gccgcggggc agcgctactg
      421 gtacaaccgg cgctggtacg agtacaccgg caacacgcac gaggagatgc agggctcggg
      481 ctggcagaag ctccaccacc ccgaccacag ccagcgcgtg taccagcgct ggatgcgcgc
      541 ggtcgagctc ggcgagccgt gggaggacat cttcccgctg cgcggcgccg acggcatcta
      601 ccgctggttc ctgtcgcgcg cggtgccgat ccgcgatgcg tcgggcgcga tcgagcgctg
      661 gttcggcacc aacaccgacg tcaccgtgcg gcggttcctc gacaacgcga ccgaggtgct
      721 gagccgcagc ctggacgagc gcgagacgct cgagcagctc gctcgcctcg cggtgccgga
      781 catcgcggac tggtgcgtcg tcgacctggt ggtgcaggac cagctcgagt acgtggcgat
      841 cgcgcacgac gacccggaca agctggaggc ggcgtggcag tacgcgcgca cccaccggtc
      901 cgaccccgcg ggccatgacg cctcgtggca gatcgtgcgg accggcaagc ccggcgtggc
      961 ggcggagatc tccgacgaga tgctcgccgc ggcctcgcgc gaccccgacc acctgcgcct
     1021 gctgcacgag ctcggcttcc ggtcgtggat cggcgccccg ctggtcgcgc gcgggaccgc
     1081 gatcggcgtg atccacctgt gcatgaacga ctccggccgc cgctaccgcg agcccgatct
     1141 ggagatcgcg gtcgagctcg ggcgccgcgc cggggtcgcg atcgacaacg cccggctgta
     1201 ccacgaggcc cagaccgcga tccgggtgcg cgacgacctg ctcgccatcg tctcgcacga
     1261 cctcaacaac ccgctgcacg cgatcgatct cggcgccacc ctgctgcacc agcagttcgg
     1321 caccgacgcg cgcgcccgca agcacatcga cgcgatccgg cgctcggccg aacgcatgga
     1381 gcgcctgatc aacgacctgc tcgacatggc gagcatcaac gccggcaagc tggcgatcaa
     1441 gccggcgccg ttcgacgccc gcgagctggt gaacgaggtg ctcgacagcc accagccgtt
     1501 cgcccacgag cgcgggatca cgatcacgcg cagctgcgac ctcgatgacg tgaccctgta
     1561 cgtcgatcgc gaccggatcg cccaggcgtt cggcaacgtg ctcggcaacg cgatcaagtt
     1621 ctgcgcgccg ggcgacgtca tcacggtcct cgccgagcgg atcggcgagg gcgtccggtt
     1681 caccttcgcc gacaccggcc ccgggatcgc accgccggat ctgccgcaca tcttcgagcc
     1741 gtactggtcg ggtcgcagcg gccgcaagaa gggcaccggg ctcggtctgt tcatcacccg
     1801 cgcgatcctc gaggcgcacg gcgggacgat ccaggtcgac agcccaccgg gcggcggggc
     1861 gagcttcgtg gtcacactcc cgatcgggaa cattggcatt gcacctgcta ctaccgatcc
     1921 acaaacaccg tgatgttgaa ctacaaccat ttgtactact tccatgtcgc ggcgtcggag
     1981 agctcgctgt cggccgccgc cgtcaagctc ggtgtgaagc agtccaccgt gagcgagcag
     2041 ctccgcacgc tggagcgtgc gctgcgccag cggctgttcg agcgcagcgc gggaggcatg
     2101 cgtctcaccg cggcgggcga ggtcgcctac gagcacacca ccctgatgtt ccgcgccggc
     2161 gatcgcctgc tccaggcgct cgggcgcgac gaggctccgg cgccgcgctc gctgcgcgtc
     2221 gggatcagcg gcgcggtcgc gcgcgcgacc tcgaccgact tcctgctgcc gctgttcgcg
     2281 ctcgacgact gcatgccgac gatctcgacc agcgagaccc tcgacctgct gcgcgacctg
     2341 cgcgccaacg agctcgatct cgtgctgtgc gagaccgagc ccaccgaggc caccgtccag
     2401 ggcctgcgcc gcgcgttgat cgagcacagt ccgctgctcg cgatcgccgc ggccgacctc
     2461 gatccgggac cggactggca ggacaccggc ctggtgcagt accgctcgag ctcgaccttc
     2521 cacttcgagg tcaaggcgtt cctcgagcag cgcgggctca agccccggat cgtcggcgag
     2581 gccgatgatc catctctcct ggtcgaggcc gccgcccgcg gccgctacgt cgtgatcgtt
     2641 ccgcgctcgg tggtccgcga cgcgctggcc gccggccggg tccggatcct ggcgcaggtc
     2701 gagtcgcacg ccggggtcca cgcgctgtat caggacggca ccgccgccga gctcgcccgg
     2761 cgcgcgatcg agaagctgat cgccaccgcg cgggccaacg tggccgcgaa ctgagcctca
     2821 cgtgccgcga tgagcgtcga tcctcagagg gttgttgcag tttcggtccg cacgacgaac
     2881 cgtggcggcg ccgcccacgc gaggccgagc gaatcggcac agggtgagcg ctggcgccac
     2941 aggcgattcc gggaaaaccg aacattgcga tcctcgcgcg gagacgacac catcgctccg
     3001 ccggcgatgc tccagccgaa cgataaagcc gtgagctggc cgacgaaggg cgatgctcac
     3061 acctcgccga gcttgcagcc ggacgcgccg ggtgctgcca tcgaggtgct cgaggccgac
     3121 gagctgctcg gcatcggcct gggctccgac atcctgatgg tggatgacga cgccgcgagc
     3181 ctcgcggcct acgaggccgc gctctcgccg ctcggccgcc ggctggtcgc cgtgcaatcg
     3241 ggcgtcgcgg cgctggccag gctgctggag caggacttcg cgctggtcct gctcgacgtg
     3301 tcgatgccgg gcatgaccgg actggagacc gcgcgccgga tccgcgagcg gccgcgcaac
     3361 cgggggttgc cgatcgtgtt catcacgggc gtcgcggggt ccaccgccgt catgctcgag
     3421 gcttacgacg ccggtgcgtt cgacttcatc gtcaagccgg tcctgcccga ggtcctgcgc
     3481 gccaaggccc gggtctacct gcagctccag gagcgcaccc aggcgctgcg gcggcactcg
     3541 gcggagctgc gcgaggccca cgcggagctc gcgcgggccg gtgagcagct gcg
//
LOCUS       DASVOR010000145         2604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_381194,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000145 DASVOR010000000
VERSION     DASVOR010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381194"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VF516_02990"
     CDS             <1..980
                     /locus_tag="VF516_02990"
                     /inference="COORDINATES: protein motif:HMM:NF013959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HEX8106662.1"
                     /translation="LGSATPGAEDWNGTASARRIDLVGPETSLGFGSAVASAGDINGD
                     GYADFLVGADGTSAAAGAAYVYLGSAMPSASAWSGAVPERRIDLTDPDDPRASFGSSV
                     TSAGDVNGDGYADFLIGARTAGSVAGSAHLYLGSAMSSAVDWNGASPARRIELTDPDG
                     ASAVFGSSVAGVGDVNGDGYADFLVGAEGVDAGSGAAHLYLGAITPNATAWNGASVAS
                     RITLTNPAGAGARFGASVASAGDVNGDGYSDFLVGAHGAGALGGASQLYLGSAMPGES
                     SWDGASPTGRMDLANPAGASAGFGVSVASTGNVDSHDGLRVPGLRVGGG"
     gene            984..>2604
                     /locus_tag="VF516_02995"
     CDS             984..>2604
                     /locus_tag="VF516_02995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106663.1"
                     /translation="MLRSQIWIPVVLVASVAACGKGDVPSDANDNAHGDTNVLVIQSD
                     AHDVSPPLTELARRPVPFVSERVHEAEPWRAIPHKQLEASGPVVDPVVQTAFGGPDIP
                     TPLTTFEGMGAGLPGFTVQSAPPDTDGDIGPNHYVQIVNSGVAIFSRTGTVLLGPVNT
                     NTLWNGFSGACATTNDGDGVVRYDRIADRWVISQFSVNGGNGPFFQCVAVSTTPDPTG
                     SYNRYQFTFDGFNDYPKMGLWPDAYYFTFNMFPNNTFAGARLCAFDRAKMLTGAAATE
                     QCFNTANTIGGLLASDLDGVTLPLAGSPNFAIAINTTTTLAFCKTHIAFTTPANSTFT
                     GPTSVTVPSFAELCNGGTCVTQPGTTNKLDSLADRLMNRASYRRFSDHDAMLVSHSVT
                     AGTGGGVRWYELRNMATTPTVFQSGTYAPDASFRWMPSMAFDQAGNIGLGFSTSSATI
                     NPSIRYTGRLVGDAAGTMGQGEATIIAGTGAQTGNLTRWGDYSSMNIDPTDDCTFWFT
                     SEYMGASGAFNWHTRIGTFKLPSCPINQPPTV"
BASE COUNT          405 a          935 c          871 g          393 t
ORIGIN      
        1 atctcggctc agcgacgccc ggtgcggagg actggaatgg gaccgcgtcc gccagacgga
       61 tcgaccttgt cggtcccgag acgtccctgg ggttcggcag cgcggtggcc agcgcgggcg
      121 acatcaacgg cgatggctac gccgacttcc tcgtcggagc ggatggcacc agcgccgccg
      181 caggcgctgc ctacgtgtat ctcggctcgg cgatgccgag cgcgagcgcg tggagcggcg
      241 ccgtgcccga gcgaaggatc gacctgaccg atcccgacga cccgcgtgcg tcgttcggct
      301 cttcggtgac cagcgccgga gatgtcaacg gcgatggcta cgccgatttc ctcatcggcg
      361 cccggaccgc cggttcggtt gcagggtctg ctcacctcta cctggggtcc gcgatgtcgt
      421 cagcggtcga ctggaacgga gcctcccccg ctcgccggat cgagttgacc gaccccgacg
      481 gcgcgagcgc tgtcttcggc tcttccgtgg ccggcgtcgg ggacgtcaac ggcgatggtt
      541 atgccgactt cctcgtcggt gccgaaggcg ttgacgcggg atcgggagcg gcccatctct
      601 acctcggagc gatcacgccg aacgcgaccg cgtggaatgg agcttcggtc gcgagccgga
      661 tcaccctgac caaccccgcc ggcgcgggcg cccggttcgg tgcctccgtg gcgagcgccg
      721 gggacgtcaa cggggacggc tacagcgatt tcctcgtcgg ggcccatggc gccggcgctc
      781 tgggcggagc gtcccagctc tacctcggct cggcgatgcc tggcgagagc tcgtgggacg
      841 gagcatcgcc caccgggcgc atggacctgg ccaatccagc cggcgcaagc gccgggttcg
      901 gggtgtcggt ggcgagcacg gggaatgtcg acagccatga tggcctgcgg gttccaggcc
      961 tccgggtcgg tggaggctga gcgatgctgc gatcgcagat ctggattcct gttgttctgg
     1021 tcgcatccgt cgcggcatgt ggcaaaggcg acgtcccgag cgacgcgaac gacaacgcgc
     1081 acggtgacac gaacgtcctc gtcatccagt ccgacgcgca cgacgtgtca cctccgctga
     1141 ccgagcttgc caggcggccg gtgcccttcg tgtccgagcg cgtccacgag gccgagccgt
     1201 ggcgcgcgat cccgcacaag cagctcgagg cgtccggccc ggtggtcgat cccgtcgtgc
     1261 agaccgcgtt tggcgggccc gacatcccga ccccgctcac gacgttcgag ggcatgggcg
     1321 ccgggctgcc cggcttcacg gtgcagtcgg cgccgcccga caccgacggc gacatcggcc
     1381 ccaaccacta cgtccagatc gtcaactcgg gcgtcgcgat cttcagccgc accggcacgg
     1441 tgctgctcgg cccggtcaac accaacacgc tgtggaacgg cttcagcggc gcgtgcgcga
     1501 cgaccaacga cggcgacggc gtcgtgcgct acgaccggat cgcggaccgc tgggtgatct
     1561 cccagttctc ggtcaacggc ggcaacggcc cgttcttcca gtgcgtcgcg gtgtcgacca
     1621 cgccggatcc gaccggcagc tacaaccgct accagttcac gttcgacggc ttcaacgact
     1681 accccaagat gggtctgtgg ccggacgcgt actacttcac gttcaacatg ttcccgaaca
     1741 acacgttcgc gggcgcccgg ctgtgcgcgt tcgaccgcgc gaagatgctg accggcgcgg
     1801 cggcgaccga gcagtgcttc aacaccgcca acacgatcgg cggcctgctc gcgtccgacc
     1861 tcgacggcgt gaccctgccg ctggccggct cccccaactt cgcgatcgcg atcaacacga
     1921 ccacgacgct cgcgttctgc aagacgcaca tcgccttcac cacgccggcg aactcgacgt
     1981 tcaccgggcc gaccagcgtc acggtcccgt cgttcgccga gctgtgcaac ggcggcacct
     2041 gcgtcaccca gcccggcacg accaacaagc tcgactcgct ggccgaccgc ctgatgaacc
     2101 gcgcgtcgta ccggcggttc tcggatcacg acgcgatgct ggtctcgcac tcggtcaccg
     2161 cgggcaccgg cggcggcgtc cggtggtacg agctccgcaa catggcgacc acgccgacgg
     2221 tgttccagtc gggcacctac gcgccggacg cgtcgttccg ctggatgccg agcatggcgt
     2281 tcgaccaggc cggcaacatc ggcctcggat tctcgacctc gagcgcgacg atcaacccga
     2341 gcatccgcta caccggccgg ctggtcggcg acgccgccgg cacgatgggc cagggcgagg
     2401 ccacgatcat cgcgggcacc ggcgcgcaga ccggcaacct gacccggtgg ggcgactaca
     2461 gctcgatgaa catcgacccg accgacgact gcacgttctg gttcacgtcg gagtacatgg
     2521 gcgcgagcgg cgcgttcaac tggcacacgc ggatcggcac gttcaagctc cccagctgcc
     2581 cgatcaacca gccgccgacg gtct
//
LOCUS       DASVOR010000146         6517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_1441,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000146 DASVOR010000000
VERSION     DASVOR010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6517
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VF516_03000"
     CDS             complement(<1..266)
                     /locus_tag="VF516_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8106664.1"
                     /translation="MTEIRMPSLGADMESGTLLEWKVAIGDRVTRGTVVGLIETEKAT
                     MELESFVEGEIEALLVEPGAKVAVGTPLARVRAAGEPAALPRTV"
     gene            complement(263..1231)
                     /locus_tag="VF516_03005"
     CDS             complement(263..1231)
                     /locus_tag="VF516_03005"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8106665.1"
                     /translation="MKLTYRDAMREALRDALRDDPRVFLVGEDIGKYGGTYAVTHGLH
                     DEFGPERVRDAPLSESAFVGAGIGAAINGMRPIVEIMTVNFSLLALDQIVNSAATLRH
                     MSGGQIEVPLVIRMATGAGRQVAAQHSHSLENWYAHVPGLRVVAPATVRDARSMLATA
                     LRERDPVIVFEHVQLYGLEDELADTAPVDLDRAAIRRPGKDATIITYGGSLPRSLAAA
                     AALAADGVDAEVIDLRTLRPLDADALVASVTRTHRAVIVDEGWRTGSLAGEIAAILGE
                     RAFWELDAPIGRVCSAEVPMPYAKHMELAALPSAEAIVAAVKAVLA"
     gene            complement(1228..2184)
                     /gene="pdhA"
                     /locus_tag="VF516_03010"
     CDS             complement(1228..2184)
                     /gene="pdhA"
                     /locus_tag="VF516_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEX8106666.1"
                     /translation="MTPGRDQAIAMLRAMIRIRRLEERCAEDYAAGKIRGFLHLYIGE
                     EAIAAGVIPALAAGDAIVATYREHGHALLRGISADAIMAEMYGKATGCARGRGGSMHL
                     FSREHRFYGGHAIVGGGLPCAVGIALADKLRATRAVTCVFFGDGAAAEGEFAESLNLA
                     ALWKLPVLFVCENNRYGMGTALERAHAEPEIHKKAAVHRIGAEVVDGMDVVAVSEAAT
                     EAIERIRGTGAPWFLECMTYRFRAHSMFDPELYRDKAEVEAWRKRDPIAAFAARMDGV
                     VTAADLAALDREAAAEVASACAFAEASPLEPVSDLLRDVRAP"
     gene            complement(2181..3914)
                     /gene="acsA"
                     /locus_tag="VF516_03015"
     CDS             complement(2181..3914)
                     /gene="acsA"
                     /locus_tag="VF516_03015"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEX8106667.1"
                     /translation="MIHPSRRSGVAYNVTDYDTARTSFRWADAAAALTGPRGLNIAEV
                     AVERHAHSGLAGKVALRWRGKHGDARDITYAGLAADMHRFGNALRALGVQPGDVVFAL
                     CPRIPALYVAALGTLAIQAVFSPLFAAFGPDPIITRVRAASGKVLLTTASLYRRRIAP
                     VRDLLPTLRHVIVVDDGGGAPAGTLGWDDVVVPASPELEIPPTPLDTLALLHFTSGTT
                     GRPKAAMHVHGAVIAHHATARMVLDLHPDDVFWCTADPGWVTGTSYGLIAPLAHGVTS
                     VVDEADFLAERWYQLLERERVTVWYTAPTAIRMLMKAGPELVRGRDLSRLRVAASVGE
                     PLNPEAVVWGAEVLGLPFLDNWWQTETGGIMIANLPCMDVRPGSMGKPLPGIEAGVVA
                     VADDRGVTELPAGEVGQLALRPGWPAMFRGYLHEDERYARCFAGGWYLTGDLARRDAD
                     GYYWFVGRGDDVIKSAGHLIGPFEVESALLEHRAVAEAGAIGVPHPIAGEIVKAFVSL
                     KPGFAASDALRDELLGHARKRLGPAVAPREIEFVADVPRTRSGKIMRRLLRARELGLP
                     EGDLSTLEGAP"
     gene            complement(4004..4243)
                     /locus_tag="VF516_03020"
     CDS             complement(4004..4243)
                     /locus_tag="VF516_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106668.1"
                     /translation="MTEPRKDWDDLRRMADELELKIRLASMDARDRWQALRPRLVKLE
                     ETIKRTGGRAGRVLTEELSSLGKALRDLRDEIARS"
     gene            complement(4240..4818)
                     /locus_tag="VF516_03025"
     CDS             complement(4240..4818)
                     /locus_tag="VF516_03025"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8106669.1"
                     /translation="MQVSDLMSRPGITCHVHDNLNVPARLMWDHDCGAVAVVGDDGKL
                     VGMITDRDVCMAAYTQDRPLGELEVNIAMARHPISARPDQDVEVVERAMAEHQVRRVP
                     VVDDDNRPIGVLSLNDLAIEADRPDTRMTDASSRVVHTLAAVCAHRFAGGPAPRAADL
                     PSDEIAPPGWFTSFYPYSLPDRAADHTKAGAP"
     gene            complement(4860..5030)
                     /locus_tag="VF516_03030"
     CDS             complement(4860..5030)
                     /locus_tag="VF516_03030"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HEX8106670.1"
                     /translation="MGLYTYERQYGSFTLPEGADLEHATSELKDGVLTLVVPKLPGAQ
                     AKKIPISTGAKS"
     gene            5285..6235
                     /locus_tag="VF516_03035"
     CDS             5285..6235
                     /locus_tag="VF516_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8106671.1"
                     /translation="MTFQKILCPVDFSLGSQQAMLLAIRLANEAAAELVLAHVWYVPP
                     LVYAEEPPFPADTLQAMIDDERRGLAAAAAEASRLGARSVTTKFLMGVPWAELVEMLQ
                     GDAAFGLVVMGTHGRTGLDRVLLGSVAEKVVRHAPCPVLTVPARGQVSEFRRVLCPVD
                     FSESSRRAVALAAELVAGGGSGITLLHSIEVPVSYSGEPRVEGFAEALDKRGTDLLED
                     WARDLRTRVSVPVTTRSRIGSPGAQILAILDDDRIFDLVVMGSHGRTGLRRALLGSVA
                     EKVVRHAGCPVVVARTRPGDQAVVTDSQPRAAAADVACTD"
BASE COUNT          906 a         2341 c         2310 g          960 t
ORIGIN      
        1 tcgacggtcc gcggcagcgc tgccggctca cccgcagcgc ggacgcgtgc gagcggggtg
       61 cccacggcga ccttggcgcc cggctcgacc agcagggcct cgatctcgcc ctcgacgaag
      121 ctctccagct ccatcgtcgc cttctcggtc tcgatcagcc cgaccaccgt gccacgcgtg
      181 acgcgatcgc cgatcgcgac cttccactcc agcagcgtcc cgctctccat gtcggcgccc
      241 agcgaaggca tccggatctc ggtcatgcga gcaccgcctt gaccgcggcg acgatcgcct
      301 ccgcgctcgg cagggccgcg agctccatgt gcttggcgta cggcatcggc acctccgcgc
      361 tgcacacccg gccgatcggc gcgtcgagct cccagaacgc gcgctcgccg aggatcgccg
      421 cgatctcgcc ggcgaggctg ccggtgcgcc agccctcatc gacgatcacc gcccgatggg
      481 tgcgggtgac cgaggcgacc agcgcatcgg cgtcgagcgg ccgcagcgtg cgcaggtcga
      541 tcacctcggc gtcgacgccg tccgcagcga gcgcggcagc tgccgccagc gaccgcggca
      601 gcgagccgcc gtaggtgatg atcgtggcgt ccttgcccgg ccgccggatc gccgcgcggt
      661 cgaggtcgac cggcgccgtg tcggcgagct cgtcctccag gccgtagagc tggacgtgct
      721 cgaagacgat caccggatcg cgctcgcgca gcgcggtcgc cagcatgctg cgcgcgtcgc
      781 ggaccgtggc gggcgcgacc acgcggaggc ccggcacatg cgcgtaccag ttctccaggc
      841 tgtgcgagtg ctgggccgcg acctggcggc cggcgccggt cgccatgcgg atcacgagcg
      901 gaacctcgat ctggccgccc gacatgtggc gcagcgtcgc ggcgctgttc acgatctgat
      961 cgagcgcgag caggctgaag ttgaccgtca tgatctcgac gatcggccgc atgccgttga
     1021 tcgccgcgcc gatcccggcg ccgacgaacg ccgactccga cagcggcgcg tcgcgcacgc
     1081 gctcggggcc gaactcgtcg tggaggccat gggtcacggc gtaggtgccg ccgtacttgc
     1141 cgatgtcctc gccgaccagg aagacccgcg gatcatcgcg cagggcatcg cgcagcgcct
     1201 ctcgcatcgc atcgcggtag gtgagcttca tggcgccctc acatcgcgca gcaggtcgga
     1261 caccggctcg agcggcgatg cctcggcgaa cgcgcaggcg ctcgcgacct cggccgcggc
     1321 ctcgcggtcg agcgccgcga ggtcggcggc ggtcaccacg ccgtccatcc gcgccgcgaa
     1381 cgcggcgatc ggatcgcgct tgcgccaggc ctcgacctcg gccttgtcgc gatacagctc
     1441 gggatcgaac atcgagtgag cgcggaaccg gtaggtcatg cactcgagga accacggcgc
     1501 gccggtgccg cggatgcgct cgatcgcctc ggtcgccgcc tcgctcaccg cgacgacgtc
     1561 catgccgtcg accacctcgg cgccgatccg gtggaccgca gccttcttgt ggatctcggg
     1621 ctcggcgtgg gcccgctcga gcgcggtgcc catgccgtag cggttgttct cgcacacgaa
     1681 cagcaccggc agcttccaca gcgccgccag gttgagcgat tcggcgaact cgccctcggc
     1741 cgccgcgccg tcgccgaaga acacgcaggt cacggcgcgc gtggcgcgca gcttgtcggc
     1801 gagcgcgatg ccgaccgcgc acggcagccc gccgccgacg atcgcgtggc cgccgtagaa
     1861 ccggtgctcg cgcgagaaca ggtgcatcga gccgccgcgc ccccgcgcgc agccggtcgc
     1921 cttgccgtac atctcggcca tgatcgcgtc ggcggagatc ccgcgaagca gcgcgtggcc
     1981 gtgctcgcgg taggtcgcga cgatcgcgtc gccggcggcg agcgcgggga tcacgcccgc
     2041 cgcgatcgcc tcctcgccga tgtagaggtg aaggaagcct cggatcttgc cggcggcgta
     2101 gtcctcggcg cagcgctcct cgagccggcg gatccggatc atcgcgcgca gcatcgcgat
     2161 cgcctggtcg cggcccggcg tcatggcgcg ccctccagcg tcgagagatc gccctcgggc
     2221 aggccgagct cgcgcgcgcg cagcaggcgc cgcatgatct tgccgctgcg ggtgcgcggc
     2281 acgtcggcga cgaactcgat ctcgcgaggc gcgaccgccg gcccgagccg cttgcgggcg
     2341 tggccgagca gctcgtcgcg cagcgcgtcg ctcgcggcga accctggctt gagcgacacg
     2401 aacgccttga cgatctcgcc ggcgatcggg tgcggcacgc cgatcgcgcc ggcctcggcc
     2461 accgcgcggt gctcgagcag cgcgctctcg acctcgaacg gaccgatcag gtgacccgcc
     2521 gacttgatga cgtcgtcgcc gcggccgacg aaccagtagt agccgtcggc gtcgcggcgc
     2581 gcgagatcgc cggtcaggta ccagccgccc gcgaagcatc gggcatagcg ctcgtcctcg
     2641 tgcaggtagc cgcggaacat cgccggccag ccgggccgca gcgcgagctg gccgacctcg
     2701 cccgccggca gctcggtcac gccgcggtcg tcggcgaccg cgacgacgcc ggcctcgatc
     2761 ccgggcagcg gcttgcccat cgagcccggc cgcacgtcca tgcacggcag gttcgcgatc
     2821 atgatcccgc cggtctcggt ctgccaccag ttgtcgagga acggcaggcc gagcacctcg
     2881 gcgccccaca ccaccgcctc ggggttgagc ggctcgccga ccgacgcggc gacccgcagc
     2941 cgcgacagat cgcggccgcg caccagctcc ggcccggcct tcatcagcat gcggatcgcg
     3001 gtcggcgcgg tgtaccagac cgtgacccgc tcgcgctcca gcagctggta ccagcgctcg
     3061 gcgaggaagt cggcctcgtc gaccacgctg gtcacgccgt gtgcgagcgg cgcgatcaac
     3121 ccgtacgagg tcccggtgac ccagcccgga tcggcggtgc accagaacac gtcgtcgggg
     3181 tgcaggtcga gcaccatccg cgcggtcgcg tggtgggcga tcaccgcgcc gtggacgtgc
     3241 atcgcggcct tcggccggcc ggtcgtgccg ctcgtgaagt ggagcagcgc gagggtgtcg
     3301 agcggcgtcg gcgggatctc cagctcgggc gacgccggca cgaccacgtc gtcccagccg
     3361 agcgtgcccg cgggcgcgcc gcccccgtcg tcgaccacga tcacgtgccg cagcgtcggc
     3421 aacagatcgc ggaccggcgc gatcctgcgc cggtagagcg acgcggtggt cagcagcacc
     3481 ttgccgctcg ccgcgcgcac ccgggtgatg atcggatcgg ggccgaacgc cgcgaacagc
     3541 ggcgagaaca cggcctggat cgcgagcgtg ccgagcgcgg cgacatacag cgccgggatc
     3601 ctggggcaca gcgcgaacac gacgtcgccg ggctggacgc cgagcgcgcg cagcgcgttg
     3661 ccgaagcggt gcatgtctgc ggcgagcccg gcgtaggtga tgtcgcgcgc gtcgccgtgc
     3721 ttgccgcgcc agcgcagcgc gaccttgccg gcgaggccgc tgtgcgcgtg gcgctcgacc
     3781 gcgacctcgg cgatgttcag gccgcgcggt cccgtgagcg cggccgccgc gtccgcccag
     3841 cggaacgagg tgcgcgcggt gtcgtagtcg gtcacgttgt acgcgacgcc gctgcgacga
     3901 gatggatgga tcaccgctgc tgtcatcgag ttcggggact gcaagcgatg caccagcgac
     3961 cgggcagctc ctccgctcga tttgcgcgaa tcctgcgtcc gcgtcagctg cgcgcgatct
     4021 cgtcgcgcag atcgcgcagc gccttgccga gcgatgacag ctcctcggtc aggaccctgc
     4081 cggcgcgccc gccggtgcgc ttgatcgttt cctccagctt cacgagccgc ggccgcaggg
     4141 cctgccagcg gtcgcgggcg tccatgctgg cgagccggat cttgagctcc agctcgtcgg
     4201 ccatcctgcg gaggtcatcc cagtccttgc ggggttcggt catggcgcac ctgctttcgt
     4261 gtgatcggct gcccggtccg ggagcgagta cggatagaag ctcgtgaacc accctggcgg
     4321 ggcgatctcg tccgagggca gatcggctgc gcgaggcgcc ggcccgcccg cgaagcggtg
     4381 cgcacacacc gcggcgagcg tgtgcacgac ccgcgaggac gcgtcggtca tccgcgtgtc
     4441 cgggcggtcc gcctcgatcg cgaggtcgtt gagcgacagc acgccgatcg gccggttgtc
     4501 atcgtcgacc acggggacac gacgaacctg atgctcggcc atcgcgcgct cgaccacttc
     4561 gacgtcctgg tcgggacgcg ccgagatcgg atgccttgcc atggcgatgt tcacctcgag
     4621 ctcgccgagt gggcggtcct gggtgtacgc cgccatgcag acatcgcgat cggtgatcat
     4681 gccgaccagc ttgccgtcgt cgccaacgac cgcgaccgcc ccgcagtcgt gatcccacat
     4741 cagcctggcc ggcacgttga ggttgtcgtg gacgtgacag gtgatccccg gacggctcat
     4801 gagatcggaa acttgcatgg cggctcctcg cgatggacgc ggatgtacgg tgtggccggc
     4861 tacgacttgg caccggtcga gatcgggatc ttcttggcct gggcacccgg cagcttgggg
     4921 accaccaggg tgagcacacc atccttgagc tcgctcgtgg cgtgctccag gtcggccccc
     4981 tcgggcagcg tgaagctgcc gtactggcgc tcgtaggtat agagacccat ggttcgctct
     5041 cctctgagtt gtgccagcag ctggagcaag cgacgcgcca ggcgacgcac ctgcgcggag
     5101 ctgcggagat cgcgatccat tcgcgttcgc gcggcgagca cggcgcaaga tctgcgcgag
     5161 cccctcaccc gcgccgcgcg ctcctctgct cgggagcgtc gaaccaccgg catgtccatg
     5221 atctcgagtt cctgtaccga aagccgattc gtgatctggc agtccgattg cagtggtgga
     5281 tcgcatgacc ttccagaaga tcctgtgccc ggtcgacttt tcgctcggat cgcagcaggc
     5341 catgctcctg gcgatccggc tggcgaacga ggcggcggcc gagctcgtgc tcgcccatgt
     5401 ctggtacgtg cccccgctgg tgtatgcgga ggagccgccg ttcccggccg acacgctcca
     5461 ggcgatgatc gacgatgagc ggcgcggtct ggcggccgcc gcggccgagg cgtccaggct
     5521 cggcgccagg tcggtgacga cgaagttcct catgggggtg ccctgggccg agctcgtcga
     5581 gatgctgcag ggcgacgccg cgttcggcct cgtggtcatg gggacccatg gccgcaccgg
     5641 cctggaccgc gtgctgctcg gctcggtggc cgagaaggtg gtccggcacg caccgtgccc
     5701 cgtgctgacc gtgccggccc gaggccaggt ctcggaattt cgccgcgtgc tgtgtccggt
     5761 cgacttttcc gagagttccc gtcgggcggt cgcactcgcg gccgagctcg tcgcgggtgg
     5821 tggctcgggc atcacgctgc tgcactcgat cgaggtaccg gtgagctatt ccggggaacc
     5881 tcgcgtggag ggctttgcag aggcgctcga caagcgtggc acggatctgc tggaggactg
     5941 ggcgcgggac ctgagaacca gagtctcggt gccggtcacg acccggtcga ggatcggaag
     6001 cccgggcgca cagattctcg cgatcctgga cgatgacagg atattcgatc tcgtcgtgat
     6061 gggcagccat ggccggaccg gcctgcgccg ggcgctgctg ggctcggtgg ccgagaaggt
     6121 ggtccgccat gccggctgcc ctgtcgtcgt cgcgcgcaca cgccctggcg accaagcggt
     6181 ggtcacggac agccaaccgc gagccgccgc tgcggacgtt gcttgcacgg actgactccg
     6241 cagctcggag tccgctgatc cgccgtccag gacccgcatg cagtgcgtga gcaggttgaa
     6301 tctgcgcaag ccattccagt gatcaggact cgaagccgta cccggcgtcg gggcatgggc
     6361 attgcaccga ggccgtgcat ggccgaacag cacgcctccc tcggcgacga gccccctgtc
     6421 gctccgcggc cgaccgggtt ccggtcggtg ctggtctgcc tcgaccggtc ccgcgctgca
     6481 gaagctgcgc tgccgctggc cacgcacctg gcgaagg
//
LOCUS       DASVOR010000147         4784 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_59817,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000147 DASVOR010000000
VERSION     DASVOR010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59817"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /locus_tag="VF516_03040"
     CDS             complement(<1..2048)
                     /locus_tag="VF516_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8106672.1"
                     /translation="MADLPALLAVLQHDPDDAQALIGLIEAARGAAPDVRTRELAAAR
                     KLLAARGRPDAVLQLIEVELAATPEHEVDRRADLLLEQGMVLDGELLDVPAARAAFDR
                     VRELRPDDSMAAEALDEIDLAASNYRKFADKYVKEADASTDRSLATGLYVSAAEAFVR
                     FEPDAEDAERYLRKALEIDPKNAKAAFHLARLYRRAGRWADLARLLEERGELAAATDE
                     RVTALLGLADVLKNRLGDPHRADAAVRRVLQLDPTQPQALRSVADAYMAAQNWPALVG
                     LYQAALKARRDEDLGMLLQIAMVLWRHVGDLDRAEEYFRRVRKLDPAHPAAIDFYRVY
                     YPAKGENQKLLALLRQIEKSPRARGDGGGTRPITVEIAELAEQQNNLEKAIEAWKQHL
                     RSEPGSRDARTALARLYRRTEKWNALLDLMKDEIERLPEGDVEGRVARLHEIVEIYRD
                     KLRLDVMVINTYNAILKIDPDNRRAGDELAAKYRALGRWNDLIAILTRKAEGQGLADA
                     ERIALLREIADLWAERFGNFANAIKPLERIVELSPGDPDAVARLKEIYTKRRQWRALI
                     DVLGREASVLAGAERRAKQGEMARLAAERLGDTRLAIEIHNRVLAEAGDAADTLTALA
                     ALYEREKRYLALVEILHRQRAGAAGKEAIALLEKLGQIYADRLHAPQQAAAAWQGVLD
                     "
     gene            2232..2900
                     /locus_tag="VF516_03045"
     CDS             2232..2900
                     /locus_tag="VF516_03045"
                     /inference="COORDINATES: protein motif:HMM:NF039623.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5995 family protein"
                     /protein_id="HEX8106673.1"
                     /translation="MSGVFPDGTTLDAVVDAMARRRFDDGDGVRYFHQVYLNVSRAVL
                     RRLDAGGFEDPAFLVALDVEFAGLYFQALDAPATAPRAWRVMFDRRRDRIGPLRFALA
                     GMNAHINRDLAVALDTTWTRLGGSLDRNSPRCRDFAAINDILAAEMATAKRELFSRFD
                     RIADVALGRVDDLLEVWSITAARDNAWSHGVALHQLRPGEAKDAAVEAFDRTASLIGR
                     LLLL"
     gene            3020..3754
                     /locus_tag="VF516_03050"
     CDS             3020..3754
                     /locus_tag="VF516_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106674.1"
                     /translation="MSSTPQPPRYAVQLLLPEPVVLDSAVVHAQMLGWHNDVQLIGSA
                     GSHFGFAIPTQDLPLLAHVFQAAPDAYAAQLDDALQWSPTWRERYNAVARCRASLVIS
                     MIAHRAVNHATMLLAFLSVLDSVLGSIDEPRATVLHWLPAQRVMPFATYRLLRMELGP
                     CGPAINVRIAQVGGSDTVADTVGLADLGLPDLQTLATGRDPAALAFRLVHLARSMFVG
                     DKLDCVWVEESSFSPPRRDALTIQLD"
     gene            complement(3763..>4784)
                     /gene="plsX"
                     /locus_tag="VF516_03055"
     CDS             complement(3763..>4784)
                     /gene="plsX"
                     /locus_tag="VF516_03055"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX8106675.1"
                     /translation="VGSDRAPGPEIAGALRAVRSSRIGSGPPALEVVLVGDAARLDAE
                     LERLGRTPEDAIRVVHAGEVVAMDDHPAHAFRSKPDSSMRVAVDRVAEGDGDAVVSAG
                     NSGAMLAIALFVLGRLPGVDRPAIVTVLPTPTGPLVLCDAGGNVEPRPSQLAQFGVIA
                     AAYDRVVHGRERPRLGLLSNGAEPGKGTQLTRDAHALLAAAHGGFHYAGYVEGSDLFR
                     GVIDCIATDGFTGNVVLKTCEGIAEGMFGLVRQELEKTPLVRLGSALVAPALRGVAKR
                     IDYHEIGGALLAGVQRPAVIAHGRSDAQAIASAIRAAARFAERALPDQLAAALTALPV
                     LPVLR"
BASE COUNT          593 a         1774 c         1713 g          704 t
ORIGIN      
        1 atgtccagca cgccctgcca cgcggccgcg gcctgctgcg gcgcgtgcag ccggtcggcg
       61 tagatctggc cgagcttctc gagcagcgcg atcgcctcct tgccggccgc cccggcgcgc
      121 tggcggtgca ggatctcgac cagcgcgagg tagcgcttct cgcgctcgta cagcgccgcg
      181 agcgccgtca gggtgtcggc cgcgtcgccg gcctcggcga gcacccggtt gtggatctcg
      241 atcgcgagcc gggtgtcgcc gagccgctcg gccgccagcc gcgccatctc gccctgcttg
      301 gcgcgccgct cggccccggc gagcacgctg gcctcgcggc cgagcacgtc gatcagcgcg
      361 cgccactgcc gccgcttggt gtagatctcc ttgagccggg cgaccgcgtc gggatcgccg
      421 ggcgacagct cgacgatccg ctcgagcggc ttgatcgcgt tggcgaagtt gccgaaccgc
      481 tcggcccaca ggtccgcgat ctcgcgcagc agcgcgatcc gctcggcgtc ggccagcccc
      541 tgcccttccg ccttgcgggt caggatcgcg atcagatcgt tccaccggcc gagcgcgcgg
      601 tacttggcgg cgagctcgtc gccggcgcgc cggttgtccg gatcgatctt gaggatcgcg
      661 ttgtaggtgt tgatcaccat gacgtcgagc cgcagcttgt cgcggtagat ctcgacgatc
      721 tcgtggagcc gggcgacccg gccctcgacg tcgccctcgg gcagccgctc gatctcgtcc
      781 ttcatcagat cgagcagcgc gttccacttc tcggtccgcc ggtacagccg ggccagcgcg
      841 gtgcgcgcgt cgcgcgaccc cggctcgctg cgcaggtgct gcttccaggc ctcgatcgcc
      901 ttctcgaggt tgttctgctg ctcggcgagc tcggcgatct cgaccgtgat cggccgggtc
      961 ccgccgccgt cgccgcgcgc ccggggcgac ttctcgatct ggcggagcag cgcgagcagc
     1021 ttctggttct cgcccttggc cgggtagtac acccggtaga agtcgatcgc cgcggggtgc
     1081 gcggggtcga gctttctgac ccggcggaag tactcctcgg cgcgatcgag atcgccgacg
     1141 tggcgccaca gcaccatcgc gatctgcagc agcatgccga ggtcctcgtc gcgccgggcc
     1201 ttgagcgcgg cctggtacag cccgaccagc gccggccagt tctgcgccgc catgtaggcg
     1261 tcggcgacgc tgcgcagcgc ctggggctgg gtcggatcga gctgcagcac gcgccggacc
     1321 gcggcgtcgg cgcggtgggg gtcgccgagc cggttcttca gcacgtcggc cagcccgagc
     1381 agcgccgtga cccgctcgtc ggtcgccgcc gcgagctcgc cgcgctcctc gagcagccgc
     1441 gcgaggtcgg cccaccggcc ggcgcgccgg tagagccgcg cgaggtggaa cgccgccttg
     1501 gcgttcttgg gatcgatctc gagcgccttg cgcaggtagc gctcggcgtc ctcggcgtcg
     1561 ggctcgaacc gcacgaacgc ctcggccgcc gacacgtaga gcccggtcgc caggctgcga
     1621 tcggtcgacg cgtcggcctc cttgacgtac ttgtcggcga acttgcggta gttcgacgcc
     1681 gcgaggtcga tctcgtcgag cgcctcggcg gccatcgagt cgtcgggccg gagctcgcgg
     1741 acccgatcga acgccgcgcg cgccgccggc acgtcgagca gctcgccgtc gagcaccatg
     1801 ccctgctcga gcaggaggtc ggcccggcga tcgacctcgt gctcgggcgt cgccgccagc
     1861 tcgacctcga tcagctggag caccgcgtcg ggccggccgc gcgcggcgag cagcttgcgg
     1921 gcggcggcga gctcgcgcgt gcggacgtcg ggcgccgccc cgcgcgcggc ctcgatcagc
     1981 ccgatcaggg cctgggcgtc gtcggggtcg tgctggagga cggcgaggag cgcgggcagg
     2041 tcggccatcg cgagcgtccg gtgatgctac cggccggacc tccgactgac aacgccgaga
     2101 gccaattcct tcgcggggtt actgcacgag ttgcgcaagg aggcgcgatc gcggcccgcg
     2161 gcccgcggcg catgcacgcg ccgcggatcg cgtgtacgat cgccgcgcac ccagcggctg
     2221 cgaggtgaac gatgagcggc gtattccccg acggcacgac gctcgacgcc gtggtcgacg
     2281 cgatggcccg gcgcaggttc gacgacggcg acggcgtccg ctacttccac caagtctacc
     2341 tcaacgtgtc ccgcgcggtc ttgcgccggc tcgacgccgg cgggttcgag gacccggcgt
     2401 tcctggtcgc gctcgacgtc gagttcgccg ggctgtactt ccaggccctc gatgcgcccg
     2461 cgaccgcgcc gcgcgcctgg cgcgtgatgt tcgaccggcg gcgagaccgg atcgggccgc
     2521 tccggttcgc gctggccggg atgaacgcgc acatcaaccg cgacctcgcg gtcgcgctcg
     2581 atacaacgtg gacccggctc ggcggctcgc tggaccgcaa ctcgccgcgc tgccgtgact
     2641 tcgccgcgat caacgacatc ctggccgcgg agatggccac cgccaagcgc gagctgttct
     2701 cgcggttcga ccggatcgcg gacgtcgcgc tcgggcgggt cgatgacctg ctcgaggtgt
     2761 ggagcatcac cgccgcgcgg gataacgcat ggagtcatgg ggtggcgctg caccagctcc
     2821 gccccggcga ggccaaggac gcggcggtgg aagccttcga ccgcactgcc agcctgatcg
     2881 gacggttgct cctgttgtga ccgaccggtc cgtcggtccc gccccacggg agtgggatct
     2941 cgaacgcgat ttcacccggg aatacgaact ggttcagcca tcgtacctat cgctgtggtc
     3001 ttgtgatacg gagacccaca tgtcatcgac gccgcaaccg ccccgctatg ccgtgcagct
     3061 cctgctgccg gagccggtcg tgctcgactc cgcggtagtc cacgctcaga tgctcggctg
     3121 gcacaacgac gttcagctga tcggctcggc cggcagccac ttcggctttg cgatcccgac
     3181 ccaggacctc ccgctgctgg cccacgtgtt ccaggccgcg cccgacgcct acgcggcgca
     3241 gctcgacgat gcgctgcagt ggagcccgac ctggcgcgag cgctacaacg ccgtggcgcg
     3301 ctgccgggcc tcgctcgtga tcagcatgat cgcgcaccgc gcggtcaacc acgccacgat
     3361 gctgctcgcg ttcctctcgg tgctcgactc cgtgctcggc tcgatcgacg agccgcgcgc
     3421 caccgtgctg cactggctgc ccgcccagcg cgtgatgccg ttcgcgacct accggctgct
     3481 gcggatggag ctcggcccgt gcggcccggc gatcaatgtc cggatcgccc aggtcggcgg
     3541 gtccgatacc gtggccgaca ccgtcgggct cgccgatctc ggcctgcccg acctccagac
     3601 cctggcgacc ggccgcgatc cggcggcgct cgcgttccgc ctggtccacc tggcgcgcag
     3661 catgttcgtc ggcgacaagc tcgactgcgt gtgggtcgag gagtcgtcgt tctcgccgcc
     3721 gcgccgcgac gcgctgacga tccagctcga ctgagggact ctctatctca gcacgggcag
     3781 cacgggcagc gcggtcagcg ccgccgcgag ctggtcgggc agcgcgcgct cggcgaaccg
     3841 ggccgccgcg cggatcgcgc tggcgatcgc ctgggcgtcg ctgcggccgt gcgcgatcac
     3901 ggccggccgc tgcacgccgg ccagcagcgc gccgccgatc tcgtggtagt cgatccgctt
     3961 ggcgaccccg cgcagcgccg gggcgaccag cgccgagccg agccggacca gcggcgtctt
     4021 ctcgagctcc tggcggacca ggccgaacat gccctcggcg atgccctcgc aggtcttgag
     4081 cacgacgttg ccggtgaacc cgtcggtcgc gatgcagtcg atcacgccgc ggaacaggtc
     4141 gctgccctcg acgtagcccg cgtagtggaa cccgccgtgc gccgcggcca agagcgcgtg
     4201 ggcatcgcgg gtcagctgcg tgcccttgcc gggctcggcg ccgttggaca agaggcccag
     4261 gcgcggccgc tcgcggccgt ggaccacgcg gtcgtaggcc gcggcgatca cgccgaactg
     4321 ggcgagctgg gacggccggg gctcgacgtt gccgcccgcg tcgcacagca ccagcggccc
     4381 ggtcggggtc ggcagcacgg tgacgatcgc cgggcggtcc acgccgggca gccggccgag
     4441 cacgaacagc gcgatcgcga gcatcgcgcc gctgttgccg gccgacacca cggcgtcgcc
     4501 gtcgccctcg gcgacccggt cgaccgcgac gcgcatcgac gagtcgggct tgctgcggaa
     4561 cgcatgcgcc gggtggtcgt ccatcgccac gacctcgccg gcgtggacga cgcggatcgc
     4621 gtcctcgggc gtgcggccca ggcgctcgag ctcggcgtcg agccgcgccg cgtcgccgac
     4681 cagcacgacc tcgagcgccg gcgggccgga gccgatccgg ctcgaccgga cggcgcgcag
     4741 ggcaccggcg atctcggggc ccggcgcgcg atcgctgccg actg
//
LOCUS       DASVOR010000148        39468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_527415,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000148 DASVOR010000000
VERSION     DASVOR010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 39468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39468
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527415"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VF516_03060"
     CDS             <1..274
                     /locus_tag="VF516_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106676.1"
                     /translation="DKDGKWEVGYSEPDPTPVPTDKRGIVLTLEEAAPMMESWAFYGG
                     FGAPDCYAVRIWTNKRVIWVTQYDGATSLDSALRHPSPCVPDMPGG"
     gene            292..750
                     /locus_tag="VF516_03065"
     CDS             292..750
                     /locus_tag="VF516_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106677.1"
                     /translation="MDTENIPAVSFESLVGEHMLDAVDTETIQVKKWSDYLEDANVIR
                     FRLDGKVYTAVEDPSDGYRSCMDKLFESTDAMRNTFAPVKVLCRINERGEYHANDTLE
                     FIDCVTGKTVLRVGTDNSDDYYPWFVAEWMPENLASNARGGEQGEEQERG"
     gene            743..1156
                     /locus_tag="VF516_03070"
     CDS             743..1156
                     /locus_tag="VF516_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106678.1"
                     /translation="MDKPDDIVGHKTFSTGDPLHPFRHEPLTRADADALIALADAQQE
                     KRAKDMPTEQDAVNAMSSAFQRLRELGWKEACYAPTDTPLKLVEPGSSGIHEGTATGE
                     WPSKSFWIFEVGDQWPSRPCLFKPSVGGSKNEEDK"
     gene            1153..1356
                     /locus_tag="VF516_03075"
     CDS             1153..1356
                     /locus_tag="VF516_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106679.1"
                     /translation="MTENAMIAIAIASIAGCTAVGAYSSSKERAAEYPGTACVRAAWN
                     QADRLECVKAQVITIKPSSSSSN"
     gene            1360..1611
                     /locus_tag="VF516_03080"
     CDS             1360..1611
                     /locus_tag="VF516_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106680.1"
                     /translation="MDKLQERLDYWRNIMTAKSGWAQNVSGPCCDKCRMTKPKDPLQA
                     LCGCRDPFQIKCNCHIAHREAVRQLIIRELEAAASCGEH"
     gene            1639..1872
                     /locus_tag="VF516_03085"
     CDS             1639..1872
                     /locus_tag="VF516_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106681.1"
                     /translation="MKVIRVLDKSGDTCIEFDEATPDALAKAKEVFDAWMGKKLPAFL
                     TKRPGGKPDEKITSFDQIEDGAETVLVPAIVAG"
     gene            1873..2646
                     /locus_tag="VF516_03090"
     CDS             1873..2646
                     /locus_tag="VF516_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106682.1"
                     /translation="MNRRDIFESVCDYHVNTDRIRVGWLSRYYGERQMVELRGMWGDR
                     SQAEELRRFIDRLVGAVVFAHHEATNDARAAIHALMPIWEREIYRFMEHLERSARRRI
                     EPRYIRYDDRMDTVQWTMAMDPARWWTDNPVEDPKAKEKARQLLLRNLDAGQEKSFKK
                     DGSFRVTAKDGKVYTIKTARSFNVVAEDGTKYCGQTVDTPVEDQMLAQKLLLEHEPEK
                     FFKNANVSPAPGAGATAYDQMIDMVNLRPGAIIRYDRAV"
     gene            2679..2933
                     /locus_tag="VF516_03095"
     CDS             2679..2933
                     /locus_tag="VF516_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106683.1"
                     /translation="MSKKKEWKVPGELAALMEEAMGAEALRAVYVTEWFGFKKALKCS
                     TIAEQRKTEFWDKVKKLYPDLPEIYNYNRADRMLRPGPAK"
     gene            3010..3168
                     /locus_tag="VF516_03100"
     CDS             3010..3168
                     /locus_tag="VF516_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106684.1"
                     /translation="MRKRVLEIVEILPNGVAAKNQAGKVRKYLRRRIFTDNKERRYGF
                     NKLYFLRV"
     gene            3351..3935
                     /locus_tag="VF516_03105"
     CDS             3351..3935
                     /locus_tag="VF516_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106685.1"
                     /translation="MRLLVACEYSGAVRRAFRARGHDAWSCDLLQAEDGSEHHIQGNV
                     VSRRIIDQGWDGLIAFPDCTYLAVSGARWMSIPWRAAAQEWALAFVRTLWALPIKKKA
                     LENPIGRLSTLWRKPDQIIQPWQFGHGETKATCLWLDGFPPLTPTQIVEGRHGRVWLE
                     PPSDDRWKERSRTYEGVAQAMAEQWGGTILEKAA"
     gene            4069..4587
                     /locus_tag="VF516_03110"
     CDS             4069..4587
                     /locus_tag="VF516_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8106686.1"
                     /translation="MSSVLDACCGSKMFWFDKADSRALFVDKRRETHPIDIGTPGTKG
                     RSPIVVDPDILADFAELPFPADSFSLVVFDPPHIERLHAKGLLTKKYGILSGDWRDML
                     RRGFAECFRVLKPEGTLIFKWAESDHPVSAILALTDEKPLFGHKSGKLGHTHWLVFMK
                     QVATSTRNRETK"
     gene            4584..4973
                     /locus_tag="VF516_03115"
     CDS             4584..4973
                     /locus_tag="VF516_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106687.1"
                     /translation="MNRTFTDDEIDRFLAFVLEGDPRRAEYSNVYGMISQLKRERDEA
                     RSGGEADAPDAKRYRFLKSIYFGADFSWQNGDDKPCPVLVFKMYDGMRISANLDRSID
                     DNIALYVRETPAGDNPPADQDTRRNDA"
     gene            4975..5163
                     /locus_tag="VF516_03120"
     CDS             4975..5163
                     /locus_tag="VF516_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106688.1"
                     /translation="MPFGEWSINFNGDFSGDVHFVSPTNERYIIPYEVVQAVVAERVR
                     RAKVAFFEDADDDSLLLS"
     gene            5192..5383
                     /locus_tag="VF516_03125"
     CDS             5192..5383
                     /locus_tag="VF516_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106689.1"
                     /translation="MRREEISALAIDAFEKGERVRMLGMTNTPTDYEDRKKAAVEMAI
                     AEAEAADAKRTLDAAIKGD"
     gene            5556..5723
                     /locus_tag="VF516_03130"
     CDS             5556..5723
                     /locus_tag="VF516_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106690.1"
                     /translation="MGAETTYYVQVVKAHLEWVEVQAVTGIEAMKEAEAMQGVLSAVE
                     YSLEKPSDEQL"
     gene            5940..6125
                     /locus_tag="VF516_03135"
     CDS             5940..6125
                     /locus_tag="VF516_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106691.1"
                     /translation="MYFVKLSVFEQHTGYSPDAVHKKIAKGVWREGVHFRRAEDGNIL
                     MDLRAYHEWVESPQQAA"
     gene            6095..7288
                     /locus_tag="VF516_03140"
     CDS             6095..7288
                     /locus_tag="VF516_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HEX8106692.1"
                     /translation="MGRKSTTGGVRPAGDRIELYFRFQGQRCRPTVDRAPTEPNLKWA
                     KRYVADILERIRHGRFDLGKEFPNYKGLARFGVARAAAKTFREYGALWLGTKGKLSPS
                     TTAGYERVLEAHWYRWFGDRHIGAVLPSEVAMKLGELPGSRKTHNNVLDPGRQIFELA
                     RGDGAIQANPCAGIAFLDLPDPEPDPYSLAEVDLILPKIRERWGEEMVDYYEWAFFSG
                     MRPSEQIEHRWDDTDVRAGHTRIQRARVEGVVKDTKTYYNRTLEHHSRAQAALQRQYA
                     RTGLAGGYVWISPFSGNGRARGAPWLDEHRQGEMFRAAVRLLRMRPRPAKNTRHTYAT
                     VLLMAGCKPSWCASQLGHSVSMFERRYAKWIPAADRGAELAKADEFTGQFTGQSDGTR
                     RRKTV"
     gene            complement(7424..7633)
                     /locus_tag="VF516_03145"
     CDS             complement(7424..7633)
                     /locus_tag="VF516_03145"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8106693.1"
                     /translation="MARYSVELSPGDEALLEMLAGGSKDRQIAAAFGWHRESVTRKVG
                     KVMDKLGALTRCEAVAIYERTIRKP"
     gene            complement(7637..8116)
                     /locus_tag="VF516_03150"
     CDS             complement(7637..8116)
                     /locus_tag="VF516_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106694.1"
                     /translation="MNPLALLPAWAWRWIAIAGVGLACAAYGAFKMHEHDQIAYNELA
                     QQFGEFKGAVKATGEKAEKDRLAKEAADKENKEKADAKNAADLAALSGMLDRARRRPV
                     SAPAACPADPSGADRFRAESERAYRDLVEGLRGEGERGSKAVIGLNTAKRWAQAPPK"
     gene            complement(8113..8562)
                     /locus_tag="VF516_03155"
     CDS             complement(8113..8562)
                     /locus_tag="VF516_03155"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HEX8106695.1"
                     /translation="MTPETEALLERDLERDEGKRSKVYKDSRGILTIGIGRNLQDRGL
                     SDDEILYLLKNDIAEHCELLDRHLPWWRNMDEVRQRALANMAFNLGVGPSDEQPTGHL
                     LTFKNTLAAMARGDYEAAANGMSSSAWAQQVGIRATRLVEMIRTGQP"
     gene            complement(8559..8861)
                     /locus_tag="VF516_03160"
     CDS             complement(8559..8861)
                     /locus_tag="VF516_03160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106696.1"
                     /translation="MAEMIESRRATDQRGWRAVTCWLAMRASDAWEWIDKRDIDKHLV
                     SLAVLYGTVKITGWAMHFAEHGDRPGLEVAAIIGAVSAPYMALQAAAIAFYFRARQ"
     gene            complement(8849..9043)
                     /locus_tag="VF516_03165"
     CDS             complement(8849..9043)
                     /locus_tag="VF516_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106697.1"
                     /translation="MALRAGRIAGCVTLGLAFLAWAGMISADEPLRIHCVNGRCSVPQ
                     KMLVEMLQRVDAAAFCGWRK"
     gene            complement(9058..9327)
                     /locus_tag="VF516_03170"
     CDS             complement(9058..9327)
                     /locus_tag="VF516_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106698.1"
                     /translation="MDGLTPAFWLQVIATVGAVAVGFGVLNANVKNLGRSLDLEREAR
                     KDHAKEDDVSFHDIRNELQSQHGRLARIEGQHDLGDKIVAAMSRH"
     gene            complement(9455..10360)
                     /locus_tag="VF516_03175"
     CDS             complement(9455..10360)
                     /locus_tag="VF516_03175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106699.1"
                     /translation="MKAKIYSLGWFLAGAAVVGFAWISDSWIFADRLPSVRAKMKQAS
                     TAENPQVVILGSSRAAFGIAPKVLSRAWGLPDSAVVNVAFPGMTPSEIEEGWRIFEPK
                     LKSAKLVIYCTDDYFLSDPPNRRSESLANEIPPVRKLGNVYDNIWAKFEKSEADLYSF
                     DKNGLLHHPMNDVDRIHDERIFERVAASWKWKRDPEIELQIAAFGRRLEANGTKIVFV
                     HLPMHSRERIALGDNGYRAGMERIAEELGTRFYYLDRPQDLGLTDESFVADPIHLRPR
                     EASIQTYQLAEVLKEQAANRRRATK"
     gene            complement(10357..11052)
                     /locus_tag="VF516_03180"
     CDS             complement(10357..11052)
                     /locus_tag="VF516_03180"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEX8106700.1"
                     /translation="MSAQTIIDKANGIIADANALATGGAWPAAVNCLGGTVPTATAFT
                     STDFWNSMCTYYGRQQGQLRNGWVPWLGTSIGQGQDVAQIHPACVNMGIGGDTLRGVL
                     NRMAGITALSRAGAVIFEYGTNDAGYDTYENISLMLDRVYGWLTGPLVVISLIPQPGC
                     SVATCDAVNGLMQTKLSGRPKCVYVDITTPLRDTDGWMKSSLSIGDRMHPNASGYALI
                     RPSIAQALQTVTA"
     gene            complement(11049..12320)
                     /locus_tag="VF516_03185"
     CDS             complement(11049..12320)
                     /locus_tag="VF516_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106701.1"
                     /translation="MTTQVYLCPTFGVGYQGFTTGGLPLNAGLLYTYIAGGTTPQATY
                     TDSIGSTLNANPIVLDADGRTPSEVWLLAGQAYRFDLKDSLGNLIKSYDNVTGINDPA
                     VASPTGVLTTVAGTNTITAVASPVPTAYAKGQRWLLTPANSITAAATLNISGVGARNI
                     MKRSSGGIVATVANDLIAANTYLLEDDGTQLIIEGVRPYSQGADVTSATTINLNTATG
                     DYVHVTGTTTITAITLAQGEERTVEFTGALTLTNGASLILPGGASITTAAGDTAIFRG
                     EAAGVVRCITYVKATSGVLPAGFGMSPLTNSLGADVTFGDTNFHDGPSVAQGTTGTWL
                     AIANITLTDSTGSANFVGKLYDGTTTKDSRAVRSSAANGAAVISLCGVFTNPAGNIRA
                     AAKDTSSASGSMLFNSSGLSLDSTIVVIRIG"
     gene            complement(12438..14510)
                     /locus_tag="VF516_03190"
     CDS             complement(12438..14510)
                     /locus_tag="VF516_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106702.1"
                     /translation="MESALSAESAKPEVVKPRRVPAGETYAGAVTGRTAVPRSRAQAP
                     TDSLDPVATAANTVTGIGTSIVGGWRGMAELARGGTLEDAVQAVHGEDENALARTFEP
                     EGAGGKKGVAAMSSPANPLNWVGVGAKKLGEVSQDLGAGPGTATTIETGINAIPLLLG
                     GRTGRTVQVRAPLSEVGKLPAKAGGEPMPLQTVPAGREPVPVGGAAPAASKPALKLVP
                     TEEKPLVKNATTEAIETKPRLKGPAAELVEEKPPEFAEADEAASTKKLPLEQQQDRAA
                     LLRRVGVENVRKSALEGDKQAAADDYQQSKLNDAGGQVMKSTLEHEREALTRHAERIV
                     EDTGGTTGMDETARMARGNAIVAPLDSLKKWFDTRTSQLYKAADESAKGVPTELPKLH
                     EAVGGDQADFLGTTEGEALLKGVKARMKSLGMVDAEGNAQPVTVAQAEKLKQYLNDQW
                     QPRTGRIIRRLKDSIDDDVLSSAGEDIYKQARALRAMRAATLDDPNGISRLMDASGPE
                     GINRAVPVEKIADAVSAMPVEQLKHVVKTLRNVPKEMQPQAQQAIAEIQAHMVSKVLE
                     EGSKQSGQWNARGVAKVLNNNSEKIGAVFSKEQIAKQKDLNDAGQILAKDQSYPGAAV
                     QEHNLVQRGAMAAVRLGAIAAGAAIGGPIGAAVGEYLGGGAAKKMGEGASLRAAQKRV
                     VKLSDFPK"
     gene            complement(14591..16087)
                     /locus_tag="VF516_03195"
     CDS             complement(14591..16087)
                     /locus_tag="VF516_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106703.1"
                     /translation="MPLTVPDQTIATQIQTPDAMKSISGLLNAAGSMQQVRSQGIDLQ
                     KKEQANKERLAVQQFMSNPDNFQTNGRIDLDKVNKAIPSIAPLTGSEVIDKLSTLSQN
                     QTQALEAKQKLTQSQRAIVAGPIGVLGRAGINDPAAYARELQSLKETNPDNPDLHRLI
                     DAYSNTLKLVPAGEHVSKTAVKASQSLMSPDAQQTSLTPTVGLTNTGGALAETISTPS
                     VAGNAPSITMTGRQQPLTLSPGSQETMQTDQLGNPIVVTKSPQGTIVNTRPAPGAGVG
                     LQLRPGDAQAIPVLEAERTSARNILSSAPIAHTTNKGILDELNNVISTGQTGGFMAKV
                     ASITGASPVGGTDAERAASAYDLIGKYTERNALEAAKAMGPGTNAGLEAAIKANGSAA
                     YNPTALKKITKLNDAIVSGAEMYQPGLEKAISANPQRGVLAKREFDQAWAQNFDPLVM
                     QIHNAQQAGDKAELQDLIKSIGGKDSARAKDVIRRAKNLEKLSADGRL"
     gene            complement(16090..17031)
                     /locus_tag="VF516_03200"
     CDS             complement(16090..17031)
                     /locus_tag="VF516_03200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106704.1"
                     /translation="MTWVAAAVGGGAIVSGLLQSNAAKSAAGTQANAANQAAQTQLQM
                     FNTVNDQNAPYRGAGNVALADILYGFGQGPNPSAGGAPYTGTVPTGADQFKYISDPSY
                     RQAFDSLNEAHTKQYGIAIPASADPSMVAQQVQAYGYNPNTVTAAPGAGAVQTGSNDI
                     GGLFTHQFNAADLNSNLAPNYQFTLDQGLGATRNAANLQTGLVSGNALKGIADYTVNK
                     AGDAYQNAFNNFTTNQNNIFNRLSTVAGFGSGANQVTANAGTTTAGNVGSAQLAGGAA
                     QAAGDIGSANALAGGISNAASWYALPKILNNGGAGPG"
     gene            complement(17028..17465)
                     /locus_tag="VF516_03205"
     CDS             complement(17028..17465)
                     /locus_tag="VF516_03205"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8106705.1"
                     /translation="MSEAITESNQRAQVSTVLRLQEEMRKLPQVELATFHYFADGMYA
                     RVVPRAAGTTIVGKVHKREHFYIITKGSVKVWSDGETRTYVAGDVIVSRPGTKRAVYA
                     LEDSICMTVHRTNKKNLKKIERDLVEPDELALFDETNTVKRLS"
     gene            complement(17462..18331)
                     /locus_tag="VF516_03210"
     CDS             complement(17462..18331)
                     /locus_tag="VF516_03210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106706.1"
                     /translation="MTTNVQFPQGPLVGDGGFVTLEWQQWFQNPQFLTLNFANPITVP
                     SGGTGLAFGTSGGVLAFTAATTIASSAALGANQIVLGGGAGVVPSTPVSLGTTTTVLH
                     GNAAGAPTWGAVSLTADVSGILPVANGGSGVATASANTVFAGPTSGGAAAPGFRALTN
                     ADIALFAPITNSLSGDVSLNNTGTYFDGPSVAQGTTGTWYATGTVTLIDTAGAAAMNV
                     KLWDGTTVIASARTTCGAASNAMSLSLSGFIASPAANIRISVNDATSTSGAIKFNASG
                     NSKDSTITAIRIA"
     gene            complement(18331..19803)
                     /locus_tag="VF516_03215"
     CDS             complement(18331..19803)
                     /locus_tag="VF516_03215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106707.1"
                     /translation="MSEFTGFVGQAYEAASRTQNDQALINWYPETDPTKFGGSPSTGA
                     PEQRGVIALYPTPGLISRLQLQYGQVRGFHTIPGGQLLFAVCGNALYSITTAYVATNV
                     GTLNSNSGRVYITDNGVSAYITDGNNRYTYNWTTGAFAVVAATDGAFVGGNVCGVVDN
                     FIFYNRPGTNQFGCTNVGDVVSGNLNFGSKIGGPDNIVSVFADHRQVLLLGEKESERW
                     INVGSFPFPFAVIPGASMQHGLHAQDSVARLGEGVAFLAQDDRGTATVVMWGATLPSP
                     QRISTFAIESAIQSYAVTADAIGYSYAQGGHEFYVLTFPTADVTWCYDISTQMWHRRA
                     WRDSQNVYHRHRSNCAVSFNGETLVGDWQNGKVYALSLTKFTDDGDPLPCVRRAPHLT
                     NDLRRQFFSDLQIQFQPGIGLQTGQGSDPTCILRWSNDGGFTFGNDHTIKMGKAGEYR
                     RRAMKRRLGWARDRVYEIEVTDPVFRVVVSANITASAGAN"
     gene            complement(19800..20729)
                     /locus_tag="VF516_03220"
     CDS             complement(19800..20729)
                     /locus_tag="VF516_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106708.1"
                     /translation="MTQALDLVTRALRAVGAYAPGDPIDDEDANDAFDMLNDMLDTWS
                     NSTQMVSYITEIIFTLTSNVYQYTIGPGGTIGGTFTGSISGTTLTVSTISAGNIALGQ
                     YLTGSGVTAGTKITQFLTGAGGVGTYQVNTSQTVGSETLTTYYQRPLRINSAFVRVAT
                     LDYPVRPLNVEQYELIGLKTLSGPWPRYLYYQPSSPVGNITFWPVPGSGEMHMFAETV
                     LSQFVTLSDTVTLPQGYNMAIRWNLAELLMPEYGKNDPVITQMIMKNAADTRAWVKRT
                     NMQPPQAASFDDALLGMHRRNDAAWIFSGGFLP"
     gene            complement(20726..21172)
                     /locus_tag="VF516_03225"
     CDS             complement(20726..21172)
                     /locus_tag="VF516_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106709.1"
                     /translation="MLRASSVLNARVTIASKPDVTAATAWTTGNSPITIFTVTGTVLI
                     TCAGVVTTPLTSTGATGTLAIGVAGTTGGLIAATTVDGTKLHTAKFVWADTSASAAIV
                     ALPGTSGWFIVSGTNIILTVATNSMTAGGMDIYALWFPLSSGANVV"
     gene            complement(21175..21504)
                     /locus_tag="VF516_03230"
     CDS             complement(21175..21504)
                     /locus_tag="VF516_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106710.1"
                     /translation="MSAFQVQGKTITFTGATVAPTPVQCVSNSGLRTPQYVLTNLGTV
                     PVWIGWGLSTGEATANAVIPTSTATYGMWLMPGNQSTISGPPDAFFTGITSSSTAVVY
                     VTPGYGE"
     gene            complement(21515..22270)
                     /locus_tag="VF516_03235"
     CDS             complement(21515..22270)
                     /locus_tag="VF516_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HEX8106711.1"
                     /translation="MKIVFNGEPPVSAEGMARNVEHCRALGLRAISVQPANEKRLAIV
                     GGGPSVLQHVEELKGWDGDILAINGACAWLRERGIDSTFFSLDPDGIVAKWAVGATKA
                     LLCSRSDPKAFEVLKDADVQVFHLHIDEKGGIVCGTSTATAAFHVAAVLGYRKVTFFG
                     CESSYEGQTHLYFNEHLPHRMVVKCGENEYLTRPDFYVQAQELSEVIRLFPSHFEERC
                     GGLLGAMVKCQEHDVTQVSPELMKELTPLKAAA"
     gene            complement(22267..22569)
                     /locus_tag="VF516_03240"
     CDS             complement(22267..22569)
                     /locus_tag="VF516_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106712.1"
                     /translation="MANSKAVGVAYSDPQFDSVGVTGNVTISGNALLGDAATDLVGVH
                     GSTGTSQATFVATFSVVAVSVSGVIGFSSSGAFSNAMTLLNALQTLLVAHGFMASS"
     gene            complement(22580..22846)
                     /locus_tag="VF516_03245"
     CDS             complement(22580..22846)
                     /locus_tag="VF516_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106713.1"
                     /translation="MSNTVASSTTTIAVGGGSPEQIGNSGGLVGAFGTTPTSQATFVA
                     TMTITGLSVSGVIGFASSAQFSAALTLLNAVQQVLVNLGFMKSS"
     gene            complement(22856..24235)
                     /locus_tag="VF516_03250"
     CDS             complement(22856..24235)
                     /locus_tag="VF516_03250"
                     /inference="COORDINATES: protein motif:HMM:NF023081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P22 phage major capsid protein family protein"
                     /protein_id="HEX8106714.1"
                     /translation="MESIKNWLLRLFEPLDRLVYSYLLSIGGAFQATDTLLTISMITN
                     ESLMVLENELTFTSQVLRTYDDAFGVDGAKIGDTLNVRRPPRFVGTTGPNLSVEDYNQ
                     TSIPVVIGDTTKYGAQFHVDTSFTTKDLRLSLGAFSENVIKPSVAAVANKVDFDGLTM
                     AKNSTANIVGVAGTPPTSLLTYLTAGAYLDAEAAPRDGGRAVVIEPFTGATIVDALKG
                     LFMPEATISQQFKKGLMGRDSAGMNWKMDQNVNVQTSGAWSAASASTLTADTTSAANL
                     GSISTGWQTTSTLVLTFGATVTLKKGDIIQIANVMPVNPQNRQVYGTKARYFVVQSDF
                     SGTGSSTGNLTVAPAIITGGQFQNCSITATSATATVTPFSIGVSATGVNSPRNILFHK
                     NAYTCAMADLPLPAGVVFAGRASSKEAGISIRVVRQYTINNDQIPARFDVLYGWAPLY
                     QELGCQVAA"
     gene            complement(24458..25489)
                     /locus_tag="VF516_03255"
     CDS             complement(24458..25489)
                     /locus_tag="VF516_03255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106715.1"
                     /translation="MSEAAVVSNNEATTTVQTIIDKQREPGRVEVVTNDNFEAFVDKK
                     LGIVKEDKPADPEEAAKKELEKVESEKKARKGQETDVEAEAGVAEHLPKDKKGKLNER
                     FSELTNARKQAEERAEKAAAEARAARDAKEAAERLAAELKAKYEPVKTDPDPEPLPSQ
                     FTDINEYSKAVKEWAADNALREAAKKAEQDRRAKEQETAVNSFKERQEAAKKEIPDYE
                     ETIKNSAVKVSNEVRDAIIESEQGPRILHHLAKNPDVADKLAQMTVGRALKEIGRLEA
                     TLGGTKADGAPQKEVKTPVAEISKAPAPITPLRGANAPVVSLSGTDAVPASMTYEDWK
                     ALRRAGKIK"
     gene            complement(25501..27624)
                     /locus_tag="VF516_03260"
     CDS             complement(25501..27624)
                     /locus_tag="VF516_03260"
                     /inference="COORDINATES: protein motif:HMM:NF027825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEX8106716.1"
                     /translation="MADPAEAVAFLKEAEDAESENRQLGLTALKFSYGDQWPQYAVAS
                     RGLDRPQLTINETNTYIKRVTNAQRQRRPRGKASPIDNMADKKVAKVITGLGRHVEVN
                     SDADNAYDTAFDFAARIGWGYWRLRHDYIAEDSRIQDIFIDVIDNPFTVYYDPNSKLP
                     DGSDAERALITDMIRKDAFKKMYPGASEGGFSSRGTGDSDPNWNTNLEIRLAEYFYVE
                     RTRAKLVYLSNGMNVWEDQLERIAPMLAQGGITIQGDRDSFKRVVKWCKQTEGEILEE
                     KTLPGRFIPVVPVYWTNVVIDSRRMRAGMVKDAMDPARMNNFWKTAITEYLALAPKAK
                     WLIAEGQDEGHENEFRNANLSSTPVLRYKPTDVAGKPSPPPQRIQPDPPPAGFIEAAF
                     MATQDLSRVMGVFDPAVRGGAQHKSDKTLNAEQGQSESTNFDGYDNLTRSIKHTWRIM
                     LSWFPTVYDTQRVQRIIGEDGRDQLVTLNEKTQAQGPDGQAIEKVLNDVTVGTYDVVM
                     DTGPGYDTLRKEGVAATMELMGTPVGEKVAAVADDLIVRNMDFPGADAIADRLAAANP
                     MSQIDEKSEVPPQTQMQIKGLQQKVEQLTGALQAAHLEIKTRNSAIEIKEHGAILREK
                     MKQEGDKEEREVTRRQKQHDSEVFALTAQNVAEINGLVKLLTSKTEHGHRLREMIVEF
                     ENSARQQDAELSAKSQQNEETTTTQ"
     gene            complement(27617..27802)
                     /locus_tag="VF516_03265"
     CDS             complement(27617..27802)
                     /locus_tag="VF516_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106717.1"
                     /translation="MSDQHSDLRAKVWKALEDLPRPVTQEAALACAQDMLTGREQADF
                     SKWFEVNGAVVVARLNG"
     gene            complement(27799..28038)
                     /locus_tag="VF516_03270"
     CDS             complement(27799..28038)
                     /locus_tag="VF516_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106718.1"
                     /translation="MTDTLGKFQQMEEQSAFDLAQYEVLGKFYEAWEALHAIPPGKLR
                     QKEKEAAAEHLVELAHMLRRMRDDKPKLLVPRFMQ"
     gene            complement(28035..29402)
                     /locus_tag="VF516_03275"
     CDS             complement(28035..29402)
                     /locus_tag="VF516_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HEX8106719.1"
                     /translation="MARKSLPAFIDYLCFGYKFAAHHRLLMQELEMVESGKIARLMVL
                     MPPGSAKSTYASVLFPPWFMGRNPEQSVLGVSNTTDLAERFSRRVRNIVGMREFTNVF
                     EGNGLSEDSAAAGNWETLRGGEFFAAGMGSAIAGRRADLGLIDDPIKTRQEADSERIR
                     QTQWDWYINDFLTRLKPGARQILIQTRWHEDDLGGRILEREAEKWRVVKLPMLAVSGS
                     DPLGRQPGDRLWPEWFTDEMVATARLDTRAWNALYQQEPVPDEGDYFKKENFGEYDSY
                     PAGLHVYGASDYAVTEGGGDFTEHGIFGIDHSGNPYVLDWWRGQTKSDAWIETQCDLI
                     RQWKPLIWFGEAGPIRRAIEPFLNKRMVDRAAYCRLEWLPSISDKEIRARSIQALASV
                     KTISLPKVAPWKADVMKQLLQFPSGKYDDAVDVFSLFGRGLEHVVPARKPRVVVDNEP
                     VGWMG"
     gene            complement(29371..29712)
                     /locus_tag="VF516_03280"
     CDS             complement(29371..29712)
                     /locus_tag="VF516_03280"
                     /inference="COORDINATES: protein motif:HMM:NF039194.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5681 domain-containing protein"
                     /protein_id="HEX8106720.1"
                     /translation="MAWEKGKSGNPAGSPKRRLITSHIERELLQAAAEGNVTKARKIA
                     EQVVSMAEGGDKWAIQFVTERTEGRPDQHVSISRSVRELTDADLTAIATGSGEGAVGE
                     AGGAEVPSRVH"
     gene            complement(29752..30123)
                     /locus_tag="VF516_03285"
     CDS             complement(29752..30123)
                     /locus_tag="VF516_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012393.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106721.1"
                     /translation="MTRFNPTQDYILVKPVERKQSEILQVVSGEKYTQGVVVATGPGK
                     KNRRGVIHPLTVKPGDFITYGCIERGYDFYPTYIESGVIYKVLQEADVAFIADPDDEA
                     HGLSDKEIAELVANSRALEIA"
     gene            complement(30342..30749)
                     /locus_tag="VF516_03290"
     CDS             complement(30342..30749)
                     /locus_tag="VF516_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106722.1"
                     /translation="METEGKSRKEICLAIGCSPSAVTRHLGAVRQYKGSRVDIEAAMK
                     DAATANPLRSAVKGYLYRYFDGGGVLLYVGISLCAISRLSGHRSSDSWYGEISRVDIE
                     KFPSIDQAKDAERKAIRAEHPKYNIDGVVPAGI"
     gene            complement(30838..31014)
                     /locus_tag="VF516_03295"
     CDS             complement(30838..31014)
                     /locus_tag="VF516_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106723.1"
                     /translation="MVYLQAKLAENDWHGVRDAAADLEVMEAKHPELKNPAPRCRLPE
                     LYCQDPDCKTHGGR"
     gene            complement(31041..31307)
                     /locus_tag="VF516_03300"
     CDS             complement(31041..31307)
                     /locus_tag="VF516_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106724.1"
                     /translation="MDVNQADIVKALRKIGAFVQPLHRLGQGVPDLLVGWRQRWLVME
                     VKRDERAELTPDEKAWIEETAGRAPVVVVTSALEAVGYLQGVRP"
     gene            complement(31322..31684)
                     /locus_tag="VF516_03305"
     CDS             complement(31322..31684)
                     /locus_tag="VF516_03305"
                     /inference="COORDINATES: protein motif:HMM:NF017578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HEX8106725.1"
                     /translation="MKPTTLRIDKCPRCGCTLRERDTVKNAHLHAILGAISKQKKWAG
                     QYLDIEAWKRLIVASYERAHGRPAEVYQAIDGDGLDVVYRRTSLMSQAEIRELILYAE
                     SWAIDNGVDIVPPERAAA"
     gene            complement(31681..32004)
                     /locus_tag="VF516_03310"
     CDS             complement(31681..32004)
                     /locus_tag="VF516_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106726.1"
                     /translation="MSQLSIDFARAARDEGIQRAADHAGEDWMSRAVKDFVQFVQEHG
                     EATCEQWRFDWLSRGNPAPATHKAYGAVPSTAAKRKLVVNTRRYVQAVSEKTHAHPVP
                     IWRPA"
     gene            complement(32001..32594)
                     /locus_tag="VF516_03315"
     CDS             complement(32001..32594)
                     /locus_tag="VF516_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106727.1"
                     /translation="MEYLRVKNWSEFQHYKDRNPPWIKLHRELLDDYEFERLQDASKA
                     HLILIWLFASQREGRIPNDPAFLKKKLGLKSDPNLKSFIDQGFLIVEQDASTPLAERE
                     QVAPRGEDIKQQSRGEPAKLNGKHRLETSAPEAFEITEPMWSWAQEQGVPHARVEPET
                     AKFLDHHKAKGSRFKDWDAAWRTWMRNSVEFAQARKH"
     gene            complement(32594..33940)
                     /gene="dnaB"
                     /locus_tag="VF516_03320"
     CDS             complement(32594..33940)
                     /gene="dnaB"
                     /locus_tag="VF516_03320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEX8106728.1"
                     /translation="MHPDEVVLENLREPPHSAEAEQSTLGALLLDNQAFDKVAWLEAG
                     AFYQDRHRRLWSAIVRLLEAGKAADLTTVSDALGEDLEKVGGLAYIGALEQNTPSAVN
                     IVRYAELVQKNALLRSIAQRSMETAERAMAGTEEPLVIADDGAAALLALQAQADSGDA
                     TEFGAALVEAVEWADNPGRGLTTGYSAVDNILRGLMPGDFIVIAGRPSMGKTALAMNI
                     AEHVAAREHVAILSLEMTKRKIAGRALRYHDSILGRDGALDHLYGLKLHIDDSSAMTL
                     GQVRARLRRVKHKHGLALAVIDYLQLLTAKAEKRHEEVATISRGLKAIAKDLNIPIIA
                     VAQLNRAAEGRPDNRPQLADLRESGQIEQDADIVAFVYREDYYRADTPWRGIAELIVR
                     KHRDGPVGTAYLKFIPEFTRFLTMDGPLPQREEPEPEKPRRTVVRADFKRDAAADR"
     gene            complement(34025..34423)
                     /locus_tag="VF516_03325"
     CDS             complement(34025..34423)
                     /locus_tag="VF516_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106729.1"
                     /translation="MTQEQLFYEDVFDVARAAVQHAGGAKKVAEILWPSKAAQQATKE
                     LLDCLNRESPRKLCIEEFMKILKLAKDAGFHQAKHWIDAELGYQPTLPADPVVERDRL
                     AENMARLTDEVKNMTRAAERLLREDIKAVK"
     gene            complement(34485..34712)
                     /locus_tag="VF516_03330"
     CDS             complement(34485..34712)
                     /locus_tag="VF516_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106730.1"
                     /translation="MDAKTFLEKHGRETTRQVCEAAETSIEYFSQIAYGHRRPSVDLA
                     KKLTKASEEAIADKRARLDVVSLLGLKEKAA"
     gene            34801..35289
                     /locus_tag="VF516_03335"
     CDS             34801..35289
                     /locus_tag="VF516_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8106731.1"
                     /translation="MENKEVRRLRLLELAKQYGTLKALAEETGTDPAHLSQIKNSTRD
                     MGEDVARRIEQALKKPAGWMDAPPGAQGPLADDELEMLEKYRRASPRWRLALRYLADV
                     PDESQDEVSESVNVLMAKIFAKPVPNQRVEEAYGRPPTRVFSTRDRNKTSLPDAQKKR
                     RA"
     gene            35382..35627
                     /locus_tag="VF516_03340"
     CDS             35382..35627
                     /locus_tag="VF516_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106732.1"
                     /translation="MGKILTLAAPMISLETVATLVDLLTEAQAGEVIGIACVVIHRAS
                     QFSIDIAGEARKTPLLTRGMVAVMDDQLAVLMGSKRP"
     gene            35731..35913
                     /locus_tag="VF516_03345"
     CDS             35731..35913
                     /locus_tag="VF516_03345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106733.1"
                     /translation="MDPYNPIIGAEHVQPEPFWKFFAGIALAACAGVVAMWFATVLLF
                     SYDDVLLAVLASVGLL"
     gene            35913..36173
                     /locus_tag="VF516_03350"
     CDS             35913..36173
                     /locus_tag="VF516_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106734.1"
                     /translation="MGAVFERELDVDPDAYGDEKMLTEAFDAADALRAHEEAAAKRQP
                     APIPQPCHECGHHYAGISCQICKTERPAWTALKNMGAMRKVA"
     gene            36170..36331
                     /locus_tag="VF516_03355"
     CDS             36170..36331
                     /locus_tag="VF516_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106735.1"
                     /translation="MIQVFKDRDLTPCMAAYEKWWNEQGKNLGIPTDRAWIIFRNGWW
                     MAEESKETA"
     gene            36328..37080
                     /locus_tag="VF516_03360"
     CDS             36328..37080
                     /locus_tag="VF516_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HEX8106736.1"
                     /translation="MSAVPAVREQHALATADSSALMKIIERASTDKDFDVAKLEKLLE
                     VKERWEAAEAKKAFVAAMAAFKAEPTTILKQKAVNIPGGAQFNHATLAAVVDGVVANL
                     SKHGFSHRWEVIQQDKLIIVSCILTHELGHQERTTLSGAPDDSGKKNAIQQIGSTVTY
                     LQRYTLMAATGLAAKDMDNDGGGAGRGNGGQMDEKALADHLANIDAAVDREGLMKAFG
                     TAWNAAQDMKDKGAQRLLNEHKQARLKALGAR"
     gene            37077..37703
                     /locus_tag="VF516_03365"
     CDS             37077..37703
                     /locus_tag="VF516_03365"
                     /inference="COORDINATES: protein motif:HMM:NF021128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEX8106737.1"
                     /translation="MKIVDFPQGTPEWAAARAGKVTASCMDKVLAKIKVGEAAARRDY
                     RAQIVAEILTGAPQDSGYINAEMQWGIEQEPFARGAYEAAQCVLVDEVGFVIHPTLER
                     AGASPDGLVGTDGLIEIKCPKTATHLQYLLDGAVPKDYEPQMMWQLACTGRAWCDFVS
                     FDPRLPEKMQMLRVRLQRDDARIAELEAEVRGFLLDVDRMLETVRRFA"
     gene            37700..37852
                     /locus_tag="VF516_03370"
     CDS             37700..37852
                     /locus_tag="VF516_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106738.1"
                     /translation="MIGRLICLFKGHKRGKRVSEATSNGGAMFADFRCPRCKATWTRK
                     AKAVAA"
     gene            37849..38046
                     /locus_tag="VF516_03375"
     CDS             37849..38046
                     /locus_tag="VF516_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106739.1"
                     /translation="MIDVPQDTPEKPLTAIDYIAALQSCEDSLEVGIFREKVPLAILH
                     DKRFKAAVQSKLSDIDRRKAA"
     gene            38043..38339
                     /locus_tag="VF516_03380"
     CDS             38043..38339
                     /locus_tag="VF516_03380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106740.1"
                     /translation="MSAAAKRHLAEIHELACVVHWNCYGQRRTADEAHHLEFVRGEHS
                     DFATVPVCAPCHDELHESRRRAFYRAHKLDDVKLLAWTIQALQAHHELSRRFAA"
     gene            38566..38886
                     /locus_tag="VF516_03385"
     CDS             38566..38886
                     /locus_tag="VF516_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106741.1"
                     /translation="MDHSEVKHLPKVDHTPAVLDDASKLLLKMADVIETRGHCQHLAE
                     DSKGRVCIVGATNYTGTIKGWCEVIDRMERAMGQRFIEWNNTPGRTKEEVVAKLRAIA
                     FLRV"
     gene            38919..>39468
                     /locus_tag="VF516_03390"
     CDS             38919..>39468
                     /locus_tag="VF516_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106742.1"
                     /translation="MAQAKKKAAKKPQPKLTIATARKVLEVVDAGLVSGMGDPVPGKM
                     CVEAAVCFALGLPHGDNPQCVGSAVRAFKIRLNDSRWSSDQARAKGMREVAIAQLGSN
                     EIDQKEFKKYLAIGIVQKIVPIAMRAAAKMVPAHAEALEAAAVACELVLDLPSARAAA
                     RAAQEKALAARKAAAAYAAAYAA"
BASE COUNT         7075 a        12527 c        12185 g         7681 t
ORIGIN      
        1 ggacaaggac gggaagtggg aggtcggata ttccgagcct gacccgacgc cggttccaac
       61 tgacaagcgg ggcatcgtcc tgacgcttga ggaggccgcg ccaatgatgg agtcatgggc
      121 tttctacggc ggcttcggtg cgcctgactg ctacgccgtg cggatctgga cgaacaagcg
      181 agtcatctgg gtaacgcaat acgacggcgc gacctcgctg gacagcgcgt tgcggcatcc
      241 gtcgccgtgc gttcccgaca tgcccggtgg ctaacaagga gaggagtaaa catggacacc
      301 gagaacatcc cggcagtcag cttcgagtcc ctagtgggcg agcacatgct cgacgcggtg
      361 gacaccgaaa ccattcaggt aaagaagtgg agcgactacc tggaagacgc caatgtcatc
      421 cgcttccggc tggacgggaa ggtctatacc gccgtcgaag atccgagcga cggctatcgc
      481 tcctgcatgg acaagctgtt cgagtctacc gacgccatgc ggaacacctt cgctccggtc
      541 aaggtgctgt gtcgcatcaa cgaacgcggc gagtaccacg ccaacgacac cttggagttc
      601 atcgactgcg tgacggggaa gaccgtcctg cgtgtaggca cggacaacag cgacgactat
      661 tacccgtggt tcgtggcgga gtggatgccc gagaaccttg ccagcaacgc aagaggaggc
      721 gagcaagggg aggagcagga acgtggataa gccggacgac atcgtggggc acaagacctt
      781 cagcactggc gatccgctcc accctttccg acatgagccg ctgacaaggg ccgatgccga
      841 cgccctgatc gcgctagccg atgcgcagca ggagaagcgg gccaaggaca tgcccaccga
      901 acaggacgcc gtaaacgcga tgtcgagcgc cttccagcgc ctccgtgagt tggggtggaa
      961 ggaagcgtgc tacgcgccaa cagacacgcc cttgaagctc gtagagcccg gcagcagcgg
     1021 tatccacgaa ggaaccgcaa ccggcgaatg gccgagcaaa agcttctgga tcttcgaggt
     1081 tggcgaccag tggccgtcga ggccgtgctt gttcaagcct tcagttggcg gatcaaagaa
     1141 cgaggaggac aagtgaccga gaacgcaatg atcgcaatcg caatcgcttc catcgctggc
     1201 tgcactgcgg tcggcgcgta ctccagctcg aaagagcgag ccgccgaata tccgggaacc
     1261 gcctgcgtcc gggccgcgtg gaatcaggcc gatcgactcg aatgcgtgaa ggctcaggtc
     1321 atcacgatca agccgtcctc ctcctcctca aactagccaa tggacaaact acaggagcgg
     1381 ctggattact ggcgcaacat catgaccgcc aagagcgggt gggcgcagaa cgtcagcggc
     1441 ccatgttgcg ataagtgccg catgaccaag ccaaaagatc cgctgcaagc cctgtgcggc
     1501 tgccgtgatc cgttccagat caagtgcaat tgccatatcg cgcaccgtga ggctgtgcgg
     1561 cagctaatca ttcgagaact tgaagcggct gcctcctgcg gtgagcacta gcaacctctt
     1621 aacgaaagga gaaacaccat gaaagtgatt cgagttctgg acaagagcgg tgacacctgc
     1681 atcgagttcg acgaggcgac ccccgacgcg ctggcaaagg ccaaggaggt attcgacgcc
     1741 tggatgggca agaagctgcc ggccttcttg acgaagcgtc cgggcggcaa gcccgacgag
     1801 aagatcacca gcttcgacca gatcgaggac ggagcggaaa ccgttctggt gccagccatt
     1861 gttgcagggt aaatgaaccg gcgcgacatc ttcgagtcgg tctgtgacta tcacgtcaat
     1921 acagacagga ttcgcgttgg ctggctcagc cgctactacg gcgagcggca gatggtcgaa
     1981 cttcgcggta tgtggggcga tcgatcccaa gccgaggaat tgcgccgttt cattgatcga
     2041 ctcgtcgggg ccgtcgtttt cgcacaccac gaagcgacaa acgatgctcg cgcggcaatt
     2101 cacgccctca tgcccatatg ggagcgggaa atttaccgct tcatggagca cctggagagg
     2161 tctgcaagac gccgcatcga gccaaggtat atccggtatg acgaccggat ggatacggtg
     2221 caatggacta tggcgatgga cccggcccgt tggtggacgg ataaccccgt cgaagatcca
     2281 aaggccaagg agaaagcgcg gcagctccta ctacggaacc tcgatgccgg gcaggagaag
     2341 tccttcaaga aggacggctc tttccgcgtc accgccaagg acggtaaggt ctacaccatc
     2401 aagacggctc gctcgttcaa cgtggtggcc gaagatggaa cgaagtattg cgggcagacc
     2461 gtggacacgc cggtcgagga tcagatgctc gcgcaaaagc tgctgctgga gcatgagccg
     2521 gagaagttct tcaagaacgc caacgtgtca ccagcgcccg gagcgggcgc cacggcctac
     2581 gaccagatga ttgacatggt gaatctacgc cccggggcaa tcatccgata tgaccgtgcc
     2641 gtctaggctt tgctactgag cagacagaga ggaagacgat gagcaagaaa aaagaatgga
     2701 aagtgcccgg agagttggcg gctctgatgg aagaggcgat gggggctgaa gccctgcgcg
     2761 ccgtttatgt taccgagtgg ttcgggttca agaaggcgct caaatgctca actattgcag
     2821 agcagcgtaa gaccgagttc tgggacaagg tgaagaagct atatccagat ctccctgaga
     2881 tctacaacta caaccgcgcc gaccggatgc tacggcctgg cccggcgaag tgatggcgac
     2941 tagcaagaca gagaggaaga aatggacgac ctacaggtag gcgacaaatt gcaggacaac
     3001 gatccgagga tgaggaagcg agtccttgag atcgtggaaa tcctgcccaa tggcgtggcg
     3061 gcgaagaacc aagccggcaa agtgcgcaag tatttgcggc gccggatctt caccgacaac
     3121 aaggaacgca ggtatggctt caacaagctg tatttcctgc gcgtataggt aagaggaatc
     3181 ggacatgaga ttgctcgaga ttttgtggcc aagcctactg ttcctggcgt ttgtcggagt
     3241 gttgtaccta catgccgtgt ggccgaggcg cccatgctgg tactgcacgg agcgcggctt
     3301 tccacactgc acatgcggcg actgatggct accttcggga ggaaagccta gtgcggctgc
     3361 tggtcgcctg cgagtattcc ggggctgtcc gtcgagcttt cagagcccgc ggacacgacg
     3421 cctggagttg cgacctcctg caggccgagg atggctccga gcaccacatc caagggaacg
     3481 tcgtatcccg caggatcatc gaccaaggct gggacgggct gattgccttc ccggactgca
     3541 cctacctcgc tgtgagcggt gctcgctgga tgtcgatccc ttggcgagct gcagcccagg
     3601 aatgggcgct agccttcgtc aggacgctct gggcgctgcc gatcaagaag aaggcgctcg
     3661 agaaccccat cggccggctc tcgacgctct ggcgcaagcc tgaccagatc atccagccgt
     3721 ggcagttcgg gcatggggaa acgaaagcta cctgcctgtg gctggacggg tttcctcccc
     3781 ttacgcctac gcagatcgtt gaagggcgcc acggccgggt ctggttggag ccgccgagcg
     3841 acgaccgctg gaaagaacgc agccgcacct atgaaggcgt cgcccaggcg atggccgagc
     3901 agtggggcgg caccatcctg gagaaagcag catgacctct acacgcgacg cctcaccgca
     3961 acctgccaag tgccagttat cgctgcacta cttgtgggtc gcctccaaag ctggttggcc
     4021 cggagccttc gcgtcctgcg tggacgagga gccgcatctc cggacagcat gagctcggtt
     4081 ctcgacgcct gctgtggcag caagatgttc tggttcgata aggccgactc acgggccctg
     4141 ttcgtggaca agcgccgtga gacgcacccc atcgacatcg ggacgccagg cacgaaaggg
     4201 cgcagcccga tcgttgttga tcctgacatc ctcgccgact tcgccgagct cccgttcccc
     4261 gctgactcgt tctcgctggt ggtgttcgac ccgccgcata tcgagcgcct gcatgccaag
     4321 gggctgctga cgaagaagta cggcatcctt tccggcgatt ggcgggacat gcttcgccgt
     4381 ggctttgcgg agtgcttccg cgtcctgaag ccggaaggca cgctcatctt caagtgggcc
     4441 gagtccgatc atccagtgtc agcgatcttg gccctgaccg atgagaagcc gctgttcggc
     4501 cacaaatcag ggaagctcgg gcatacgcac tggctcgtat ttatgaagca ggttgctact
     4561 tccactcgca accgggagac gaaatgaacc gcacctttac cgacgacgag atcgaccgct
     4621 tcttggcctt cgtgctggag ggcgatccac gccgcgccga gtacagcaac gtctacggca
     4681 tgatctctca gctcaagcgg gagcgcgacg aagcgaggag tggcggtgaa gctgacgcac
     4741 cggacgcgaa gcggtatcgg ttcctgaagt cgatttactt cggggccgat ttcagttggc
     4801 agaacggcga tgacaagccg tgtccggtgc tggtgttcaa gatgtacgac ggcatgcgga
     4861 tcagcgccaa cttggaccgc tccatcgacg acaacatcgc gttatacgta agggaaaccc
     4921 ctgcggggga taatcctcct gcggaccaag atacgaggag gaacgatgca taacatgccg
     4981 tttggcgagt ggtcgatcaa cttcaacgga gacttttctg gcgacgtgca cttcgtcagc
     5041 cccaccaatg agcgttacat catcccgtat gaggtcgtgc aggccgtcgt ggcggagcga
     5101 gtgaggcggg cgaaggtcgc attcttcgag gatgccgacg acgactcgct gctgctctcc
     5161 taaaggcggc gacatagacg aggagtttgg aatgagacga gaggaaatat ctgcgctcgc
     5221 aattgatgct ttcgagaagg gcgagcgggt acgcatgctc ggcatgacga acacgcctac
     5281 cgactatgag gatcgcaaga aggccgcggt cgagatggca atagctgaag ccgaggcagc
     5341 cgacgcaaag cggacactgg acgctgccat caagggcgac taacaaagag gaaagcctcg
     5401 atgtggcgct actggcgatg cgtactggct caaagcttca tgaaagccct gctctggatt
     5461 gtcctgatcc tcgctgccgg gtatgccgcc tatatgacga acgagtgtcc aaaggatcaa
     5521 acgtgcgtat ccgctccgca gaaggctaaa cggtaatggg cgctgaaacc acctactacg
     5581 tgcaggtcgt caaggctcat ctcgaatggg tcgaagtgca ggccgtgacc gggatcgaag
     5641 ccatgaagga agctgaagca atgcagggtg ttttgtcggc ggtggaatac tcgctggaga
     5701 agccgagcga tgagcagctc tagctggcac cagaaacctc cttctgacgt tcgctcgctg
     5761 aaggacgcca tacgcgctgc tgagcgctta cgcggcgatc tgaagctcat cgttcaggtg
     5821 aacgcatccg attgggacaa ggtgatacta gtggacgaaa tctaccggct acggcgcagc
     5881 ggtggccggt cctcctcctc tggagattcg caaagctaat gctgccggcg ccgctccccg
     5941 tgtacttcgt caagctctcg gtcttcgagc agcacaccgg ctattcgcct gacgccgtgc
     6001 acaagaagat cgcgaaaggg gtttggcgcg aaggggtaca cttccggcgg gcggaggacg
     6061 ggaacatctt gatggacctt cgcgcgtacc acgaatgggt cgaaagtcca caacaggcgg
     6121 cgtaagaccg gcgggcgacc gcatcgagct ctacttccgc ttccaggggc agcgctgcag
     6181 gccgaccgtc gatcgagcgc cgacagagcc gaatctgaaa tgggcgaagc gctacgtcgc
     6241 cgacatcctc gagcgcatcc gccacggccg ctttgacctc ggcaaggagt tcccgaacta
     6301 caagggcctg gctcgcttcg gcgtagcgcg cgccgcggca aagaccttcc gcgagtacgg
     6361 ggccctatgg ctcggcacca agggcaagct gtcgccgtcg acaacggccg gctacgagcg
     6421 cgtcctcgag gcgcactggt atcgctggtt cggcgatcgg cacatcggtg ccgtgctgcc
     6481 ctccgaggtc gccatgaagc tcggggaact gcctggcagc cggaagacgc acaacaacgt
     6541 cctcgacccc gggcggcaga ttttcgagct cgcgcgcggc gacggcgcca tccaggcgaa
     6601 cccatgcgcc ggcatcgcct tcctcgacct acccgacccg gagcccgacc cctacagctt
     6661 ggccgaggtg gacctgatcc tgccgaagat ccgcgagcgc tggggcgagg agatggtcga
     6721 ctactacgaa tgggccttct tcagcggcat gcggccatcc gagcagatcg agcaccggtg
     6781 ggacgacacc gacgtccgcg ccggccacac gcgcatccag cgggcccggg tcgagggggt
     6841 ggtcaaggac acgaagacat actacaaccg cacgctcgag caccacagcc gcgcgcaggc
     6901 ggccctgcag cgccagtacg cccgcactgg cctggcgggc ggctacgtgt ggatctcgcc
     6961 gttcagcggc aacgggaggg cccgcggcgc gccatggctg gatgagcacc ggcagggaga
     7021 gatgttccgg gcggccgtca ggctgctcag gatgcgcccg cgcccggcga agaacacccg
     7081 gcacacctac gccaccgtgc tcctcatggc gggctgcaag ccgtcctggt gcgcctccca
     7141 gctcgggcac tcggtctcga tgttcgagcg caggtacgct aaatggatac cggcggcaga
     7201 ccgcggcgcc gagctcgcca aggccgacga gttcactgga caattcactg gacaatcgga
     7261 cggcacgagg cggcgaaaga cggtatgacg ccagccgttt tcactctgta ggcttagtgg
     7321 taagcgggtt cgattcccgc cgcctccacc acctgattcc attgcagaat caagcaactt
     7381 atcgccttgc ggcaagtcgg ctatcggact tcaactggac aaatcagggc ttcctgatcg
     7441 tgcgctcgta gatcgccacc gcctcgcagc gcgtgagcgc gccgagcttg tccatcacct
     7501 tcccgacctt gcgcgtcacc gattcccgat gccagccgaa agcggcggcg atctggcggt
     7561 ccttgctccc gccggcgagc atctcgagca gcgcctcgtc gcccggtgat agctccaccg
     7621 agtagcgcgc cacggcctac ttcggcggag cctgcgccca gcgcttcgcc gtgttgagac
     7681 cgatcacggc tttgctgccg cgttcaccct ctcctcgtaa gccttcaaca aggtctcgat
     7741 acgctcgctc ggactcggcg cggaatcgat cggctcctga aggatctgcg ggacaggcgg
     7801 cgggggcgga cactggacgg cgacgggcac ggtcgagcat cccgctaaga gcagcaagat
     7861 cggcagcatt cttcgcatcg gccttctcct tgttttcttt gtctgcggcc tccttggcga
     7921 ggcggtcctt ctccgccttc tcgccggttg ccttcaccgc tcccttgaac tcgccgaact
     7981 gctgcgcgag ctcgttgtag gcaatctggt cgtgctcgtg catcttgaac gcgccgtagg
     8041 ccgcgcaggc gagaccaacg ccagcgatgg cgatccacct ccaagcccac gcagggagaa
     8101 gcgcaagcgg gttcacggct gccccgtcct gatcatctcg accagccgag tcgcccggat
     8161 gcctacctgc tgcgcccaag ccgacgagct cataccgttg gctgccgcct cgtagtcgcc
     8221 tctggccatc gcggcgagcg tgttcttgaa agtgagcaga tgcccggtcg gctgctcgtc
     8281 gctcggcccg actcctaggt tgaaggccat gttcgcaaga gcgcgctggc gcacctcgtc
     8341 catgttcctc caccacggca aatgacggtc gagcagctcg caatgctcgg cgatgtcgtt
     8401 cttgagcagg tagaggatct cgtcgtcgga aagacctcgg tcttggaggt tcctaccgat
     8461 gccgatcgtg aggatgccgc ggctgtcctt atagaccttc gagcgcttgc cctcgtcgcg
     8521 ctcaagatca cgctctagaa gagcttcggt ctctggggtc attgccgcgc cctgaagtag
     8581 aaggcgatcg ccgcagcctg cagcgccatg taaggagccg aaacggcgcc gatgatggcc
     8641 gccacctcaa ggcccggtcg atccccatgc tcagcgaagt gcatagccca gccggtgatc
     8701 ttgaccgtgc cgtagagcac cgcgagcgaa accaggtgct tgtcgatgtc gcgcttgtct
     8761 atccattccc atgcatcgga tgcccgcatg gcgagccagc acgtcaccgc ccgccatccg
     8821 cgctgatccg tggcgcgtcg gctctcgatc atttccgcca cccgcaaaac gcggccgcat
     8881 cgacccgttg cagcatctcg accagcatct tctgcggaac ggagcatcgg ccgttgacgc
     8941 aatgaatccg cagcggctca tcggcagaaa tcatcccggc ccatgcgagg aacgccagtc
     9001 cgagagtcac gcacccggcg atccggccgg cgcgcagcgc catgcagcgc ggactcatca
     9061 gtgcctcgac atggccgcca cgatcttgtc gccgagatcg tgctgcccct cgatgcgggc
     9121 aagacgtccg tgctgagatt gcagttcgtt cctgatgtcg tgaaagctca cgtcgtcctc
     9181 tttcgcgtga tccttgcggg cttcgcgctc aaggtcgagg cttcgcccga ggttcttcac
     9241 gttcgcattg aggacgccga atccaaccgc caccgccccc acggtcgcga ttacttgcag
     9301 ccagaatgcc ggggtaagcc catccatttc aggactcctg gatagttcac gtcgggagcc
     9361 gttggtgtag gcttggcctc gcctgtacgg gcagggaggg cctgtcgcca atgctcggct
     9421 ccatcgagcg gaggggccag gtgatatttg cgtatcactt ggtcgccctc cttctattgg
     9481 cggcttgttc ttttaaaact tccgcgagtt ggtaagtctg tattgatgct tctctcggac
     9541 gcaggtggat aggatctgca acgaaggact cgtccgtaag ccccaaatct tgcgggcggt
     9601 ccaggtagta gaacctcgtg ccgagttctt cggcaatgcg ctccatcccg gctcgatagc
     9661 cgttgtcgcc tagcgcaatt ctttccctgg aatgcatcgg cagatggacg aatacgatct
     9721 tcgtcccgtt cgcttccaaa cgtctgccga aagccgcgat ctgcagttca atctccgggt
     9781 cacgcttcca cttccagctc gccgctaccc gttcaaaaat gcgctcgtcg tgaatgcggt
     9841 caacatcatt catggggtga tgcagcaggc cgttcttgtc gaagctgtat agatcggctt
     9901 ccgacttctc gaacttggcc cagatgttgt cgtagacgtt cccgagtttt cttaccgggg
     9961 ggatttcgtt tgccaagctc tcggaacgcc tgtttggcgg gtcgctgagg aagtagtcat
    10021 ccgtgcagta gatgaccagc tttgcggact tgagcttggg ctcgaatatc cgccagccct
    10081 cctcgatctc cgatggagtc atgcctggaa acgcgacatt cacgaccgcg ctatccggca
    10141 atccccaagc ccttgagagc actttcggag cgatgccgaa agctgccctc gaggagccga
    10201 ggatgacgac ctgcggattt tcagcggtac tggcctgctt catcttcgcg cgcaccgatg
    10261 ggagccggtc agcgaagatc cagctatccg agatccacgc gaatccgaca actgcggcgc
    10321 ccgccaggaa ccagccaagg ctgtagattt tcgctttcaa gcagtcaccg tctgaagcgc
    10381 ctgcgctatt gatggacgaa tcaaagcata gcccgaagcg ttggggtgca tccgatcacc
    10441 gatgctgagt gaagatttca tccagccgtc ggtatctcgt agtggcgtcg tgatgtccac
    10501 ataaacgcac ttcggacgac ctgataactt ggtctgcatg aggccgttca cggcgtcaca
    10561 ggtcgcaacg gaacaacctg gttgcggaat gagggagatg accaccagcg gcccagtcag
    10621 ccagccgtaa acgcggtcca gcatcaggct gatgttctcg taggtgtcat agccggcgtc
    10681 gttggtgccg tactcgaaaa tcacagcacc agctcggcta agagcggtga tacccgccat
    10741 gcgattgagg acgcctcgca gggtatctcc accgatcccc atgttcacgc aagccggatg
    10801 gatctgggcg acatcctgcc cctgcccgat tgaagtcccg agccacggca cccagccatt
    10861 gcgaagctga ccctgctgcc gcccgtagta tgtgcacatc gagttccaaa aatccgtcga
    10921 tgtgaaagcc gttgccgtag gcacggtccc gccgaggcaa ttcaccgccg cgggccatgc
    10981 gcctccggtc gccagcgcat tcgcgtcggc aatgatgccg ttcgctttgt cgatgatggt
    11041 ctgcgcgctc atccgatcct tatgacgacg atagtgctgt ccagcgaaag gccggaagaa
    11101 ttgaacagca tcgagccgct cgcgctgctg gtgtctttgg ctgcggctct aatgttcccg
    11161 gccggattcg taaagactcc gcacaacgag atgaccgccg ccccgttcgc ggccgagctc
    11221 cgcaccgccc ttgaatcctt ggtggtggtc ccgtcgtaca gcttcccgac gaaattcgcc
    11281 gatccggtgg aatcggtcaa agtgatattg gcgatagcaa gccatgtccc cgtcgttccc
    11341 tgcgcaacgc tcgggccgtc atggaaattc gtatccccga aggtgacatc cgcgccgagg
    11401 ctgttcgtga gcgggctcat gccgaaaccg gccgggagca cgccggaggt cgccttgaca
    11461 taggtgatgc agcgcactac gccagccgcc tcgcctctga agatcgccgt atccccggct
    11521 gcagtcgtga tgcttgcccc gcccggaaga atgagcgagg ctccattcgt cagagtcagg
    11581 gcgccggtga actcgaccgt tctttcctcg ccctgagcca aggtgatcgc cgtgatggtc
    11641 gtcgtgccgg tgacgtgaac atagtcgccg gtcgcggtgt tgaggttgat cgtggtcgca
    11701 gaggtaacgt ccgcgccttg cgaatacggc ctcacaccct cgatgatgag ttgcgtcccg
    11761 tcatcttcca gaagataggt attcgccgcg atgaggtcgt tcgccaccgt tgcgacgatc
    11821 ccgcccgagg agcgcttcat gatgttgcgc gcaccgaccc cggagatatt cagcgtcgcc
    11881 gcggcggtga tcgagttggc cggcgtcagc agccagcgct gccccttcgc ataggccgta
    11941 ggaaccggac tcgctactgc ggtaatcgtg ttcgtgcctg cgacggtcgt gagaacgccg
    12001 gtaggcgacg caacggccgg gtcgttgatc cccgtcacgt tgtcgtagga cttgatcagg
    12061 ttcccgaggc tgtccttgag gtcgaacctg tacgcctgcc cggccagcag ccacacctcg
    12121 ctcggcgttc tgccgtccgc gtccagaaca atcggattcg cgttcagcgt gctcccgatg
    12181 ctgtccgtgt aggtcgcctg cggggtggtt ccccctgcga tgtaggtgta aagtaggccg
    12241 gcgttgagcg gcaggccccc ggtggtgaag ccttggtagc cgacgccgaa cgtggggcac
    12301 agatatactt gcgtggtcat ttatggaccc tatttcgact tgggtttttc tcgcctggct
    12361 ctggaaaaag atcgatgacg ccaggctccc tccctggatg agcctcgaag aatttgagcg
    12421 gtggcgtcgt cgtcatgtca cttcggaaaa tccgagagtt tcactacccg cttctgagcc
    12481 gcgcgaagcg acgcgccttc ccccatcttc ttagcggcac cgccgccaag atattccccg
    12541 accgcagcac caatcggacc gccaatcgcg gcgccggcag caatagcacc taaacgaaca
    12601 gcagccatcg ccccgcgctg cacgaggttg tgctcctgca ccgcagcccc cggatagctc
    12661 tggtccttgg cgaggatctg ccccgcatcg ttcaagtcct tctgcttcgc gatctgctcc
    12721 ttggaaaaca ccgcgccgat cttctcggag ttgttattca gcaccttggc aacgccgcgc
    12781 gcgttccact ggccggattg cttcgagccc tcctccagca ccttgctcac catgtgcgcc
    12841 tggatctcgg cgatggcctg ctgcgcctgc ggctgcatct ctttcggcac gttgcgcagc
    12901 gtcttcacca catgcttgag ctgctcgacc ggcattgcgg ataccgcgtc cgcgatcttc
    12961 tcgacaggaa ccgcccggtt gataccctcc ggccccgagg cgtccatgag gcgggaaatg
    13021 ccgttcgggt cgtccaaggt cgcggcacgc atcgcccgaa gcgcgcgcgc ctgcttatag
    13081 atgtcctcgc cggccgagga gagaacatcg tcgtcaatcg agtccttcag cctgcggatg
    13141 atgcggccgg tgcgcggctg ccattggtca ttgaggtact gcttcagctt ctccgcctgc
    13201 gccacggtga cgggctgagc atttccttct gcgtccacca tcccgagcga cttcatgcga
    13261 gccttgacgc ccttcagcag cgcctcgccc tccgtggtcc cgaggaagtc tgcttgatct
    13321 ccaccgaccg cttcgtgcag ctttgggagc tcagtcggga cgcctttcgc gctctcatcg
    13381 gcggccttgt agagctggct cgttctcgtg tcgaaccact tcttcaggct gtcaagcggg
    13441 gcgacgatgg cgttgccgcg ggccatccgc gccgtttcgt ccatgccggt cgtgccgccg
    13501 gtgtcctcca cgatcctttc ggcgtgccta gtcagtgcct cgcgctcgtg ctcgagcgtg
    13561 gacttcatga cctggccgcc ggcgtcgttc agcttcgact gctggtagtc gtccgccgca
    13621 gcctgtttgt cgccctctag ggcgctcttc ctgacattct ccacgccaac tcggcggagg
    13681 agtgctgccc ggtcctgttg ctgctccagc ggcaatttct tggtgctagc agcctcgtca
    13741 gcttccgcga actccggcgg cttttcttcc accagctcgg cggccggccc cttgaggcgc
    13801 ggcttcgtct cgatcgcctc ggtggtggcg ttcttcacca gcggcttctc ttcggtcggg
    13861 acgagcttca gggctggctt ggaagccgca ggcgcggcgc cgccgaccgg tacaggttcg
    13921 cgcccagccg gcaccgtctg cagcggcata ggctcgccgc cggccttggc cggcagcttc
    13981 ccgacctcgg acagtggcgc ccttacctgt accgtcctcc cagtcctgcc gcccagcagt
    14041 agggggatgg cgttgatgcc tgtttcgatc gtggtagcgg ttccgggccc ggccccgaga
    14101 tcctgcgaga cctcgcccaa tttcttagcg ccgacgccga cccagttgag cgggttcgcg
    14161 ggggacgaca tcgccgccac acccttcttg ccgccggcgc cctccggctc gaaagtcctg
    14221 gcgagcgcgt tctcgtcctc gccgtggacg gcctggacgg catcttccag cgtgccgccg
    14281 cgggcgagct ccgccatgcc cctccagccg ccgacgatgc tggtgccgat gcccgtcacc
    14341 gtgttggctg cggtcgccac cgggtcgagc gagtccgtag gcgcctgtgc gcgcgagcgc
    14401 gggaccgccg tgcggccggt cacagcaccg gcataggtct cgcccgccgg cacccggcgc
    14461 ggtttcacga cctccggctt tgctgactcg gcactcagcg ctgattccac ggaatcggcc
    14521 aaccccgcag gggcaggcgc atagcggctc gagttcccga gcgcggactc gacggattca
    14581 gcgaggccca ttacagcctt ccatcggcgg agagtttttc aaggttcttc gcccggcgga
    14641 tcacgtcctt ggcgcgagcg gagtccttgc cgccgatcga cttgatgagg tcttgcagct
    14701 cggccttgtc gccagcctgc tgggcgttgt ggatctgcat cacgagcggg tcgaagttct
    14761 gcgcccatgc ctggtcgaac tcgcgcttgg cgagaacgcc cctttgcggg ttggccgaga
    14821 tcgccttctc gagaccgggc tggtacatct ccgccccgct cacgatggcg tcgttgagct
    14881 tcgtgatctt cttcagcgcg gtcgggttgt aggccgccga tccgttcgcc ttgatcgcgg
    14941 cttcgaggcc ggcgttcgtc cccggcccca ttgccttcgc agcttccagg gcattgcgct
    15001 cggtgtactt cccgatcagg tcgtaggccg aagctgcgcg ctccgcatcc gtaccgccga
    15061 caggagaggc cccggtaatg ctcgccacct tcgccatgaa cccgccggtc tgtccggtgg
    15121 aaatcacgtt gttgagctcg tccagaatcc ctttgttcgt cgtgtgcgcg atgggcgccg
    15181 aggagaggat gtttcgcgcg ctcgtgcgct cggcctccag cacggggatc gcctgcgcgt
    15241 ctccgggcct gagttgtagc ccgaccccag caccaggcgc tggccgcgta ttgacgatgg
    15301 tcccctgcgg gcttttcgta acgacgatcg ggttgccgag ctggtccgtc tgcatcgtct
    15361 cctggctgcc gggggagagc gtgagcggct gctggcgtcc ggtcatggtg atgctcggcg
    15421 catttcccgc caccgaaggc gtgctgatcg tctccgcgag cgccccaccg gtattcgtga
    15481 ggccaactgt cggcgtgagc gaggtctgtt gcgcatccgg cgacatgagc gactgcgaag
    15541 ccttcaccgc cgttttggag acgtgctcgc cggccgggac caatttgagc gtgttcgagt
    15601 aggcgtcgat cagccggtgc aggtccgggt tgtccgggtt cgtttccttg agactctgca
    15661 gttccctcgc gtaagctgcg gggtcgttga tgccggcgcg accaagaacc ccgatagggc
    15721 cagcgacgat cgcgcgctgg ctttgcgtga gcttctgctt cgcttcgagc gcctgcgtct
    15781 ggttctgcga gagcgtcgag agcttgtcga tcacctcgct ccccgtcagc ggcgcaatcg
    15841 acggaatggc cttgttgacc ttatcgaggt cgatccttcc gttggtctgg aagttgtccg
    15901 ggttcgacat gaactgctga accgcgagcc gctccttgtt ggcctgctcc ttcttctgaa
    15961 ggtcgattcc ctgcgaccgc acctgctgca tggagccggc agcattgagc agcccggaaa
    16021 tgctcttcat cgcatccggg gtctggatct gcgtcgcaat cgtttggtct ggaacggtga
    16081 gaggcatggt taacccggac ctgcgccacc gttattcaga attttcggga gcgcgtacca
    16141 gctcgcggcg ttggagattc ctcccgccaa ggcgttcgcg ctgccgatgt cacccgcagc
    16201 ctgcgcagcg ccgcctgcaa gctgagcact cccgacattg cctgcggtgg tcgtgccagc
    16261 gttcgccgtg acctgattcg cgcccgaacc gaagcccgcg accgtggaaa ggcggttgaa
    16321 gatgttgttc tggttcgtgg tgaagttgtt gaaggcgttc tggtaggcat ctccagcctt
    16381 attgaccgtg tagtcggcga tgcccttgag ggcatttccg ctgaccagtc cggtctgcag
    16441 gttcgccgcg ttccgcgtcg cgcccaaccc ctgatccaag gtgaactgat agttcggcgc
    16501 gaggttcgag ttgaggtcgg ccgcgttgaa ttggtgcgtg aagaggccgc cgatgtcgtt
    16561 cgagccagtc tgcacagcgc cggcaccagg cgcggccgta accgtgttcg ggttgtagcc
    16621 gtatgcctgc acttgctgcg ccaccatcga aggatcggcc gaggcaggaa tggcgatccc
    16681 gtactgcttc gtatgcgcct cgttcagcga atcgaaagcc tgccggtacg aagggtcgga
    16741 aatatatttg aactgatctg cgccagtcgg gacggttccg gtatagggag cgccgccggc
    16801 ggaaggattc ggcccttgac caaatccgta gaggatgtcc gcgagcgcca cattcccagc
    16861 cccgcgatat ggcgcgttct ggtcattgac cgtgttgaac atctgcagtt gcgtctgcgc
    16921 cgcctgattc gcggcgttcg cttgcgtacc tgcagcggat ttggcggcgt tggattgcag
    16981 gaggccgctg acaatggccc cgcctccaac ggctgcggct acccaggtca tgaaagcctc
    17041 ttcacggtgt tggtctcatc gaaaagcgca agctcatccg gctccacgag atcgcgctcg
    17101 atcttcttca ggttcttctt gttggtgcgg tggaccgtca tgcagatcga atcctcgagc
    17161 gcgtacaccg ctcgcttggt ccccggcctg gaaacgatca catctccggc gacgtaagtc
    17221 ctagtctcgc catcgctcca caccttcacg ctgcccttgg tgatgatgta gaagtgctcg
    17281 cgcttgtgga ccttgccaac gatggtggtc ccggccgcac gcggcacgac tcgcgcgtac
    17341 atcccatcgg cgaagtagtg gaacgtcgca agctcgacct gcggcagttt ccgcatctcc
    17401 tcctgcaaac gcagaacggt agagacctga gctcgctggt tcgattctgt gatggcttcg
    17461 ctcatgcgat ccgaatcgcg gtgatggtcg agtccttgga attgccggag gcgttgaact
    17521 tgatcgcccc ggaggtcgaa gtcgcatcgt taaccgaaat gcgaatattc gctgcagggc
    17581 ttgcgatgaa ccctgaaaga gatagggaca tggcgttact cgccgctccg caagtagtcc
    17641 tggcgctcgc aatgaccgtt gtcccatccc agagctttac attcatcgcc gcggcgccag
    17701 cggtatcaat caatgtcact gtcccggtcg cgtaccatgt tccggttgtc ccctgcgcaa
    17761 cgctagggcc gtcgaaatag gttccggtgt tgttcagtga tacatcgcca ctcagggaat
    17821 tggtgatcgg cgcgaatagc gctatatccg cattggtgag cgcccggaac ccaggcgcag
    17881 cagcgccgcc ggaagtcggc cctgcgaata ccgtattcgc ggacgccgta gctactcctg
    17941 aaccgccgtt ggcgacagga agaatcccgc ttacgtcggc ggtgaggctc acggctcccc
    18001 acgtcggagc gccggctgcg tttccgtgaa ggacggtagt ggtcgtcccg agacttacag
    18061 gcgtggacgg aacgactcct gcgccaccgc cgagaacgat ctgatttgcg ccaagcgcag
    18121 cagacgaggc aatagtggtc gccgcggtga acgcgagcac accacccgac gttccgaaag
    18181 cgagtcccgt cccaccactc ggaaccgtga tcggattggc gaagttgagc gtgaggaact
    18241 gcggattctg gaaccactgc tgccattcga gcgtgacgaa tccaccgtcg ccaacaagtg
    18301 ggccctgcgg gaactggacg ttggtggtca tcagttcgcc ccagccgagg ctgtgatgtt
    18361 ggccgagacc acgacccgga aaacggggtc cgtcacttcg atctcataca cgcggtcgcg
    18421 cgcccagccg agtcttcgct tcatcgcgcg gcggcgatac tctccggcct tccccatctt
    18481 gatcgtgtga tcgttcccga acgtaaagcc gccgtcgttc gaccagcgaa ggatgcaggt
    18541 agggtccgag ccctgcccgg tctggagccc gattccaggc tgaaactgga tctggaggtc
    18601 ggagaagaac tgccggcgca gatcattcgt gagatgcgga gcgcggcgca cgcacggcag
    18661 cgggtctccg tcgtcggtga atttcgtgag ggagagcgca tagactttcc cgttttgcca
    18721 atctccgacg agcgtctccc cgttgaagga aactgcgcag ttgctccggt gacggtggta
    18781 gacgttctgc gagtcgcgcc atgccctgcg gtgccacatc tgcgtcgaga tgtcgtaaca
    18841 ccaggtaacg tcagcggtcg ggaaggtgag gacatagaac tcatgccctc cttgcgcata
    18901 gctatagccg atcgcgtccg cagtaacggc atagctctgg atggccgact cgatcgcgaa
    18961 agtcgagatt ctctgaggac tcgggagggt cgccccccac atcacgaccg tagccgtgcc
    19021 gcggtcatct tgcgcgagga acgccacgcc ctcgccaagc ctcgcgactg aatcctgcgc
    19081 gtgcaggccg tgctgcatcg aggcgccggg gatcacggcg aaggggaaag gaaaggagcc
    19141 gacgttgatc caccgctcgc tctccttctc gccgaggagc agcacttgcc gatggtctgc
    19201 gaagaccgaa acgatgttgt ccgggccgcc gatcttcgag ccgaagttga gattgccgga
    19261 aaccacatct ccgacattcg tgcagccgaa ttgattcgtg ccaggccggt tgtagaagat
    19321 gaagttgtca acgactccgc agacattgcc cccaacaaaa gccccatccg tggccgccac
    19381 caccgcgaac gcgccggtgg tccagttgta ggtgtagcgg ttgttgccgt ccgtgatgta
    19441 ggcggaaacg ccgttatcgg tgatgtagac gcgcccggag ttgctgttga gcgtcccgac
    19501 attcgtcgcg acgtaggccg tcgtgatcga atagagcgca ttcccgcaga ccgcaaaaag
    19561 cagttggccg cctggaatgg tgtggaagcc cctgacttgc ccgtactgga gctgcagcct
    19621 cgaaatgagc cccggcgtag gatatagcgc gatcacgcct cgctgctccg gagctccagt
    19681 cgaaggcgac ccgccgaact tcgtggggtc ggtctcggga taccagttga tcagcgcttg
    19741 gtcgttctgc gtcctcgagg ccgcctcgta agcctgcccg acgaagccag tgaactcgct
    19801 cacggcagga agcctccgct gaagatccag gcggcatcgt tcctgcggtg catcccgagc
    19861 agcgcgtcat cgaaggacgc tgcttgcggc ggctgcatgt tggtccgctt cacccacgcg
    19921 cgcgtatccg cggcgttctt catgatcatc tgcgtgatga ccggatcgtt cttcccgtac
    19981 tccggcatca gcaactccgc gagattccag cggatcgcca tgttgtagcc ctgcggcagc
    20041 gtcaccgtgt cggagagcgt tacgaattgg gagagcaccg tctcggcgaa catgtgcatc
    20101 tcgccagagc ccggcaccgg ccagaacgtg atgttcccga ccgggctgga gggctggtag
    20161 tagaggtagc gcggccacgg ccccgatagc gtcttcaggc cgatcagctc gtactgctcg
    20221 acgttgagcg gtcgcaccgg gtaatcgagc gtcgccacgc gcacgaaggc gctgttgatc
    20281 cgcagaggac gctggtagta ggtcgtcagc gtctcgctcc cgaccgtctg cgaggtattg
    20341 acctggtagg tgccgactcc gccggcgccg gtcaggaatt gcgtgatctt cgtgccggcc
    20401 gtcacccccg agccggtgag gtactgccct agcgcgatgt tccccgcgct gatggtcgag
    20461 acggtgagcg tcgtgcctga aatcgacccg gtgaacgtgc cgccgatcgt cccgccgggc
    20521 ccgatcgtgt actggtagac gttgctcgtg agcgtgaaga tgatctccgt gatgtaggag
    20581 accatctgcg tcgagttcga ccaggtgtcg agcatgtcgt tgagcatgtc gaacgcatcg
    20641 ttcgcatcct catcgtcaat gggatcgcct ggggcatagg cgccgaccgc ccgcagggct
    20701 ctcgttacga gatcaagtgc ctgggtcaaa cgacattcgc cccggaggac agcgggaacc
    20761 agagggcgta gatgtccatg ccccccgcag tcatgctgtt ggtcgcgact gtcaggatga
    20821 tgttggtccc ggagacgatg aaccagccgg aggttccggg aagggcgacg atcgccgccg
    20881 aggcggatgt gtcggcccac acgaatttag ccgtgtgcag cttcgtgccg tccacggtgg
    20941 tcgcggcgat caagccgccg gtcgttccgg cgacgccaat ggcgagcgtg ccggtggcgc
    21001 cggtcgaggt cagcggcgtc gtcaccacgc cggcgcaggt gatcagcacc gtgccggtca
    21061 cggtgaagat cgtgatcggg gagttgccgg tggtccaggc cgtcgccgcc gtaacgtcgg
    21121 gcttggaggc gatcgtaacg cgcgcgttga ggaccgagct cgctctcagc atcgctattc
    21181 tccgtaccct ggggtcacat agacgaccgc ggtgctcgag gacgtgatgc cggtgaagaa
    21241 cgcatccggc ggcccgctga tcgtggattg gttccccggc atgagccaca tgccgtaggt
    21301 cgccgtcgag gtcgggatca ccgcgttggc cgtcgcctcg ccggtcgaga ggccccagcc
    21361 gatccacacc ggcaccgtgc cgaggttcgt gaggacgtac tgcggcgtcc tgagacctga
    21421 attgctcacg cactgcaccg gagtcggcgc cacggtcgcc ccggtaaagg tgatcgtctt
    21481 tccctgtacc tgaaacgcgc tcatctcaag ctccctatgc ggccgccttg agcggcgtca
    21541 gttccttcat cagctcaggc gaaacctgcg taacgtcgtg ctcctggcac ttcaccatcg
    21601 cccccagcaa cccgccgcag cgctcctcga agtgcgaggg gaaaaggcgg atcacttccg
    21661 acagttcttg cgcctggacg tagaaatccg gccgcgtgag gtattcgttt tcgccgcact
    21721 tcacgaccat ccggtgcggg aggtgttcgt tgaaatagag gtgcgtctgc ccctcgtagc
    21781 tcgactcgca accgaagaac gtcaccttgc ggtagccgag caccgcggcg acgtggaagg
    21841 ctgcggtagc ggtgctggtg ccgcaaacga tcccgccctt ctcatcgata tggaggtgga
    21901 acacctgcac atcggcgtcc ttcagcacct cgaacgcctt cgggtcgctc ctcgagcaga
    21961 gcagggcctt cgtcgcccct accgcccact tcgcaacgat cccgtccggg tcgagcgaaa
    22021 agaacgtgct atcgattccg cgctcgcgca gccaggcgca ggcgccgttg atcgccagga
    22081 tgtcgccatc ccagcccttc agctcctcga cgtgctgcag aaccgaaggc ccaccgccga
    22141 cgatggcgag ccttttctca ttcgccggct ggacagaaat ggcgcgcagg ccgagcgccc
    22201 tgcaatgctc gacgttgcgc gccatacctt cagccgatac tggcggctct ccgttgaaaa
    22261 cgatcttcac gaagacgcca tgaagccgtg cgcgacgagg agcgtctgca gggcattcag
    22321 gagcgtcatc gcgttggaga aggcgccgga gctcgaaaac ccaatcaccc cggacaccga
    22381 caccgccacg accgagaacg tggcgacgaa cgtggcctgc gaagtcccgg tgctgccgtg
    22441 gacccccacc agatccgtcg ccgcatcccc gagaagcgcg ttcccgctga tcgtgacgtt
    22501 gccggtgacg cccaccgagt cgaactgcgg gtcgctgtat gcgaccccta cggctttgct
    22561 gttcgccata ggggtctcct tacgaggact tcatgaagcc gaggttgacg agcacctgct
    22621 gcaccgcgtt cagcagcgtc agcgcggccg agaactgcgc cgagctggcg aagccgatca
    22681 ccccggaaac cgacagcccg gtgatcgtca tcgtcgctac gaacgtggct tgcgaggtgg
    22741 gggtcgtgcc gaaagccccg accaggccgc cggagttgcc gatctgttcc ggcgaacccc
    22801 cgcctacggc gatcgtggtg gtgctggatg cgacagtgtt cgacatgatc cctccttatg
    22861 ccgcgacctg gcagccgagc tcttggtaga gcggcgccca gccgtacaga acatcgaaac
    22921 gcgccgggat ctggtcgttg ttgatcgtgt actgccgaac aacgcggatt gagattccgg
    22981 cctcctttga agacgccctg cccgcgaaca ccacgccggc gggcagcgga aggtccgcca
    23041 tcgcgcaggt gtaggcgttc ttgtggaaga ggatgttgcg cggcgagttc acgccggtcg
    23101 ccgacacgcc gatcgagaac ggcgtcaccg tggcagtcgc agaggttgcg gtgatgctgc
    23161 agttctggaa ctggccgccg gtgatgatcg ccggcgccac cgtgaggttg cccgtcgagg
    23221 agcccgtacc gctgaagtcc gactgcacca cgaagtagcg agccttcgtc ccgtaaacct
    23281 ggcggttctg cgggttgacc ggcatcacgt tcgcgatctg gatgatgtcg cccttcttca
    23341 gcgtgaccgt ggcgccgaag gtgagaacca gcgtcgaggt cgtctgccag cccgtcgaga
    23401 tgctgccgag gttggccgcc gaggtcgtat ccgccgtcag ggtcgaggcc gaagccgccg
    23461 accaggcgcc ggaggtctgg acgttgacgt tctggtccat cttccagttc atgccggcgg
    23521 aatcgcggcc catcaggccc ttcttgaact gctggctgat cgtcgcctcg ggcatgaaca
    23581 ggcccttcag cgcgtccacg atcgtcgcgc cggtgaaggg ctcgatcacc accgccctgc
    23641 cgccgtcgcg cggagccgcc tcggcatcga ggtaggcgcc agcggtcagg taggtcagga
    23701 gcgaggtcgg cggcgtgccg gcgacgccga cgatgttggc ggtcgagttc ttcgccatcg
    23761 tcaggccgtc gaagtccacc ttgttcgcca ccgcggccac tgagggcttg atgacgttct
    23821 cggagaaggc cccgagagac agtcgcagat ccttcgtggt gaacgacgta tcgacgtgga
    23881 actgcgcgcc gtacttcgtg gtgtcaccga tcacgaccgg gatcgaggtc tggttgtagt
    23941 cctcgaccga caggttgggt ccggtggtgc cgacgaagcg cggggggcga cgcacgttca
    24001 gcgtgtcgcc gatcttcgcg ccatcgacgc cgaaggcatc gtcataggtg cggagaacct
    24061 gactcgtgaa agtcagctca ttctccagga ccatcaacga ctcgttggtg atcatggaga
    24121 tggtgagcag cgtatcggtc gcctggaagg cgccgccgat gctcaggagg tacgagtaaa
    24181 ccagccggtc cagcggctcg aagagccgga ggagccagtt cttgatgctt tccatttaca
    24241 gctcctagtt ggttgagaag gaaaacgctt gcttcgcgct ctgcttctcg aggagctgtt
    24301 ctcggatgta cccggaatcc gtcccggtct ccgtccttgg tgcgcccaag gctaagcggt
    24361 gatttctttt ggcgccgggt gcgcctactt ctccccggat acgacgctta atacaccgag
    24421 actatttttc ttagggaaaa tgtttttctt acttttgcta cttgatcttt cccgcccgac
    24481 gaagcgcctt ccagtcctcg taggtcatgc tcgccgggac cgcatcggtg ccactcaggc
    24541 tcaccacggg cgcgttcgcg ccgcgcagcg gcgtgatggg cgccggcgcc ttggaaatct
    24601 ccgccaccgg cgtcttgacc tccttctgcg gcgcgccatc ggccttcgtg cctccgaggg
    24661 tcgcctcgag ccggccgatc tccttcagcg cgcgtccgac cgtcatctgc gccagcttgt
    24721 cggccacgtc cgggttcttg gcgaggtggt gcaggatgcg cggcccctgc tcgctctcga
    24781 tgatcgcgtc ccgaacctcg ttcgagacct tgaccgcaga gttcttgatg gtctcctcgt
    24841 agtcgggaat ctccttcttg gccgcctcct ggcgctcctt gaaggaattg accgccgttt
    24901 cctgctcctt cgcccggcga tcctgctcgg cctttttcgc ggcctcacgc agcgcgttgt
    24961 cagcggccca ttccttgacc gccttggaat attcattgat gtcggtgaac tggctgggca
    25021 gcggctccgg gtccgggtcg gtcttcaccg gctcgtattt cgccttcaac tcggccgcca
    25081 agcgttccgc ggcctccttt gcgtcacgcg cggcgcgcgc ctccgccgcg gccttctccg
    25141 ccctttcctc ggcctgcttc cgggcgttgg tgagctcgga gaagcgctcg ttcagcttcc
    25201 ccttcttgtc ctttggcagg tgctcggcga cgccggcctc cgcctcgaca tcggtctcct
    25261 ggcccttgcg cgcctttttc tcggactcga ccttctcgag ctccttcttg gcggcttcct
    25321 cggggtccgc gggcttgtct tccttcacga tgccgagctt cttgtccacg aacgcctcga
    25381 aattgtcgtt cgtcaccacc tccacgcggc cgggctcgcg ctgcttgtcg atgatcgtct
    25441 ggacggtggt cgtcgcttcg ttgttgctca ctacggccgc ttcggacatg cctgctcctg
    25501 ttattgggtg gtggtggttt cttcgttttg ctgggacttc gccgaaagtt cagcgtcttg
    25561 ctggcgcgcg ctgttctcga actccacgat catctcgcgc aggcggtggc cgtgctcggt
    25621 cttgctcgtc aagagcttca ccaggccgtt gatctcggcg acgttctgcg cggtaagcgc
    25681 gaagacctcg gaatcgtgct gcttctgccg gcgcgtcact tcgcgctctt ccttgtcgcc
    25741 ctcctgcttc atcttctctc gcaggatcgc gccgtgctcc ttgatctcga tggcgctatt
    25801 gcgggtcttg atctcgaggt gcgccgcctg gagcgcaccg gtgagctgct cgaccttctg
    25861 ctgcaggccc ttgatctgca tctgcgtctg cggcggcacc tcggacttct catcgatctg
    25921 cgacatgggg ttggcggccg caagcctgtc ggcgatcgca tcggcgccgg ggaagtccat
    25981 gttccgcacg atcaggtcgt cggccaccgc ggcgaccttc tccccgaccg gcgtccccat
    26041 gagctccatc gtggccgcga cgccctcttt tctcagcgtg tcgtagcccg gccccgtgtc
    26101 catcaccacg tcgtaggtgc cgaccgtcac gtcgttcagc accttctcga tcgcctgccc
    26161 gtcaggccct tgcgcctgcg tcttctcgtt cagcgtgacg agctggtcgc gcccgtcctc
    26221 cccgatgatc cgctgcacgc gctgcgtgtc gtagaccgtg gggaaccacg acagcatgat
    26281 ccgccaggta tgcttgatcg agcgggtcag gttgtcgtag ccgtcgaaat tcgtactctc
    26341 ggactggccc tgctcggcgt tgagcgtctt gtccgacttg tgctgcgcgc cgcccctcac
    26401 cgccgggtcg aacaccccca tcacgcgcga gagatcctgc gtcgccataa aagcggcctc
    26461 gatgaacccg gccggcggcg ggtcgggctg gatgcgctgc ggcggcggag acggcttccc
    26521 tgccacgtcg gtcggcttgt agcgcagcac cggcgtcgag gagaggttgg cgttcctgaa
    26581 ctcgttttcg tgcccctcgt cctgcccctc ggcgatcagc cacttcgcct tgggcgcgag
    26641 ggcgaggtac tccgtgatcg ccgtcttcca gaagttgttc atgcgcgccg ggtccatcgc
    26701 gtccttcacc atccccgccc gcatccggcg cgagtcgatc accacgttcg tccagtacac
    26761 cggcacgacc gggatgaagc gcccaggcag cgtcttctcc tcaaggatct cgccctcggt
    26821 ctgcttgcac cacttcacga cgcgcttgaa gctgtcgcgg tcgccttgga ttgtgatgcc
    26881 gccttgggcc aacatcggcg cgatccgctc gagttggtcc tcccatacgt tcatgccgtt
    26941 gctgagatag accagcttcg ctcgcgtgcg ctcgacgtag aaatactcgg cgagccggat
    27001 ctcgaggttc gtgttccagt tcgggtcaga atccccggtg ccgcgggagc taaagccacc
    27061 ctcgctcgcc cccggataca tcttcttgaa ggcgtccttc ctgatcatgt ccgtaatcag
    27121 ggcgcgctcg gcgtccgacc catccggcaa ctttgagttc gggtcgtagt acaccgtgaa
    27181 cgggttgtcg atgacatcga tgaagatgtc ctggatgcgg gagtcctcgg cgatgtagtc
    27241 gtggcgcagg cgccagtacc cccagccgat ccgcgccgcg aaatcgaacg ccgtgtcgta
    27301 ggcgttgtcc gcgtccgaat tgacctcgac gtgccggccg agcccggtga tgaccttggc
    27361 gaccttcttg tccgccatgt tgtcgatcgg cgaagccttg cccctcggtc tgcgctgccg
    27421 ctgggcgttc gtgaccctct tgatgtaggt gttcgtctcg ttgattgtga gctgcgggcg
    27481 gtccaggcca cgagaggcga cggcgtattg cggccactgg tcgccatagc tgaatttcag
    27541 cgcggtcagc ccaagctgcc ggttctcgga ctcggcgtcc tcggcctcct tcaggaacgc
    27601 gacggcttca gcgggatcag ccattgagcc tcgccaccac cacggcgccg ttgacctcga
    27661 accatttcga gaagtccgcc tgctccctgc cggtcagcat gtcctgcgcg cacgcgagcg
    27721 ccgcttcctg cgtcaccggg cgcggcagat cctccagcgc cttccacacc ttcgcccgaa
    27781 gatcgctgtg ctggtcgctc attgcatgaa cctcggcacg agtagcttgg gcttgtcgtc
    27841 gcgcatccgg cgcagcatgt gggcgagctc gaccaggtgc tccgcggcgg cctccttctc
    27901 cttctgccgc agcttgccag gcgggatggc gtgcagcgcc tcccacgcct cgtagaactt
    27961 cccgagcacc tcgtattgcg cgaggtcgaa ggcgctttgc tcctccatct gctgaaactt
    28021 gccgagcgtg tcggtcatcc catccacccg accggctcgt tgtccaccac gacgcgcggc
    28081 ttgcgcgccg gcaccacatg ctcgagcccg cggccaaaga ggctgaacac gtccaccgcg
    28141 tcgtcgtact tccccgaggg gaactggagg agctgcttca tcacgtccgc cttccacggc
    28201 gcgaccttcg gcaggctgat cgtcttcacc gaggcaaggg cctggatgga gcgcgcgcgg
    28261 atctccttgt cgctgatgct cggcagccac tccaggcggc agtaggcggc gcggtccacc
    28321 atgcgcttgt tcaggaaggg ctcgatagcc cggcgaatcg gcccggcctc gccgaaccag
    28381 atgagcggct tccactgcct gatgaggtcg cactgtgtct cgatccaggc gtcggatttg
    28441 gtctgccccc tccaccaatc gaggacgtag gggttgccgc tgtggtcgat cccgaaaatg
    28501 ccgtgctcgg tgaagtcccc gccgccctcc gtcacagcgt agtcgctggc gccgtagacg
    28561 tgcagcccgg cgggatagga gtcgtactcg ccgaagtttt ccttcttgaa gtagtctccc
    28621 tcgtccggca cgggctcctg ctggtagagc gcgttccagg cccgagtatc gaggcgcgcg
    28681 gtcgctacca tctcgtcggt gaaccactca ggccaaagac ggtctccggg ttgccgcccg
    28741 agcgggtcac ttcctgacac agcaagcatt ggcagtttga cgacgcgcca cttctcagcc
    28801 tcgcgctcga ggatgcggcc accgagatcg tcctcgtgcc aacgggtctg gatgaggatc
    28861 tgccgagcgc cgggcttgag gcgcgtgagg aagtcgttga tgtaccaatc ccactgtgtt
    28921 tgccgaatac gctcagagtc agcttcttgc cgcgttttga tgggatcatc gataagtcca
    28981 agatcggctc gccgtccagc aatagccgac cccattccag cggcaaagaa ctcaccaccg
    29041 cgtagagttt cccaattgcc cgctgcagcc gagtcctcgc tgagcccgtt gccctcgaag
    29101 acgttcgtaa attcacgcat cccgacgatg ttccgcaccc ggcggctaaa gcgctccgcg
    29161 aggtccgtgg tgttcgagac gccgaggacc gactgttcag gattcctccc catgaaccag
    29221 ggcgggaaga gcacgctcgc gtaggtggat ttcgccgagc ccggcggcat cagcaccatc
    29281 agccgagcga tcttgccgct ctcgaccatc tccagctctt gcatcaagag ccggtggtgc
    29341 gccgcgaact tgtagccgaa gcatagatag tcaatgaacg cgggaaggga cttccgcgcc
    29401 acccgcctcg ccaacagctc cttcgccgct tcctgtcgcg atggctgtga ggtctgcgtc
    29461 ggtgagctct cggacgcttc ggctgattga gacatgctgg tccgggcgtc cctcagtgcg
    29521 ctcggtgacg aactggatcg cccacttgtc gccgccttcc gccatgctca cgacctgctc
    29581 ggcgatcttc cgcgccttcg tgacatttcc ttctgcggcc gcttgcagca gctcgcgctc
    29641 gatatgcgac gtgatcaggc gccgtttggg gctgccggct ggattccctg atttgccctt
    29701 ctcccatgcc atttgcgaac agcgcctatt tctttgattt cttagcgagc actacgcgat
    29761 ctccagcgct ctactgttgg ccacgagctc ggcgatttcc ttgtccgaga ggccgtgcgc
    29821 ttcgtcgtcc gggtcggcga tgaaggccac gtccgcctcc tggagcactt tgtagatcac
    29881 cccgctttcg atgtaagtag ggtagaagtc gtagccgcgc tcgatgcagc cgtaggtgat
    29941 gaaatcaccc ggcttgaccg ttaagggatg gatcacaccc ctccggttct tcttgccggg
    30001 tccggtggca acgaccacgc cttgcgtgta cttctcgcca gagacgacct gcaggatttc
    30061 ggactgcttg cgctcgacgg gcttgacgag gatgtagtcc tgcgtcgggt tgaagcgggt
    30121 catggaaaaa tctgcgcgat gtaatggccg cgcttggcga gaagaatccc accttcagcc
    30181 caatatccat tgcgcgacca gccgacaaaa gaaagcccag gcagaacgcc cgggcttcta
    30241 aatgcgcgga tacccgcgcg gcggaggaga tttccttgca cgttgaacgt ttgtaacgcg
    30301 caacgctaag aaatgcagca tcttaagaaa tctgtaaaaa attagattcc ggccggaact
    30361 actccatcga tgttgtactt gggatgttcg gcacgaatcg cttttctttc tgcatccttg
    30421 gcttgatcaa tggatggaaa tttctctatg tccacccgag atatttctcc ataccatgaa
    30481 tctgaagagc gatggccgga tagccttgaa attgcacaca aactgatgcc cacatagagc
    30541 aaaactccgc cgccatcaaa atacctgtac aggtatccct taacagccga ccgcaatgga
    30601 tttgcggtgg ccgcatcctt catagcggcc tcaatatcga cacgacttcc cttgtactgc
    30661 cgcacggcgc cgaggtgtcg agtcaccgcg gagggtgagc agccgatcgc aaggcaaatt
    30721 tccttgcgcg atttcccctc ggtctccatg cgcttcatcg tctcgacctc ttcctgggaa
    30781 atgtctcttc ttcttgttct catttcgtag cccctttgtt ccacgttgaa ccttttctta
    30841 gcgaccccca tgcgttttgc aatcaggatc ttggcaatag agttccggca gcctgcaccg
    30901 tggcgccgga ttcttcagct ctggatgctt cgcttccatg acttcgagat ccgccgcggc
    30961 gtcgcgcacg ccgtgccagt cgttctccgc cagcttggcc tgcaggtaaa ccacgagcgc
    31021 gctatggcga agttcgggag tcaagggcgc actccctgca aatagcccac agcctcaagt
    31081 gcgctcgtca ccacgacgac cggcgcgcgg ccggcggtct cctcgatcca cgccttctcg
    31141 tccggtgtga gttcggctcg ctcatcccgc ttgacctcca tcaccagcca gcgctggcgc
    31201 caacccacga gcaaatctgg gaccccttgg ccgaggcggt gtagcggctg cacgaatgcg
    31261 ccgatcttgc gcagagcctt cacgatatct gcctggttca cgtccactcg cgcagcacgc
    31321 ctcacgccgc agccctctcc ggcggcacga tgtccacccc gttgtcgatc gcccacgatt
    31381 cggcgtagag gatcaactcg cggatttccg cctgcgacat gagcgaagtg cggcggtaca
    31441 ccacgtcaag cccatccccg tcgatcgcct ggtagacctc cgccgggcgt ccgtgcgcgc
    31501 gctcgtagga cgcgacgatc agccgcttcc aggcttcgat gtcgaggtat tgcccggccc
    31561 acttcttctg cttcgagatc gccccgagga tcgcgtgaag gtgtgcgttc ttcaccgtgt
    31621 cgcgctcgcg cagcgtgcag ccgcagcgtg ggcatttgtc gatccgtagc gtcgtcggct
    31681 tcacgccggc ctccagatcg gcaccggatg cgcgtgggtt ttctcgctca ccgcttgaac
    31741 gtagcggcgc gtgttgacca ccagcttgcg cttcgcggcc gtggaaggga ccgcgccgta
    31801 agccttatgc gtcgctggcg caggattgcc gcgggagagc cagtcgaagc gccactgctc
    31861 gcatgtggcc tcgccatgct cctgcacgaa ctggacgaaa tccttgaccg ccctcgacat
    31921 ccagtcctcg ccggcgtggt cggcggcgcg ctgaataccc tcatctcgag cggcgcgcgc
    31981 aaagtcgatg gagagctggc tcaatgcttc ctcgcctgcg cgaactcgac cgagttccgc
    32041 atccaggtgc gccaagctgc atcccaatcc ttgaacctcg agcccttcgc cttgtggtga
    32101 tcgaggaact tggccgtctc cggctcgacc cgggcatgcg gaaccccctg ctcttgagcc
    32161 catgaccaca tcggctcggt aatctcgaac gcctccggcg ctgacgtttc gagtcgatgc
    32221 ttcccgttca gcttcgcggg ctctcctcta ctctgctgct ttatatcctc tcctctaggt
    32281 gcaacttgct cgcgttctgc tagcggagtg ctagcgtctt gctccacaat caggaaccct
    32341 tgatctataa aagatttcag gttcggatcg ctcttgaggc cgagcttctt cttgaggaac
    32401 gccgggtcgt tcggaatgcg cccttcccgc tggctggcga agagccaaat cagtatcagg
    32461 tgcgccttgc tagcatcctg caagcgctca aattcgtagt cgtcgagcaa ttcgcggtgc
    32521 agcttgatcc agggcggatt gcggtccttg tagtgctgga actcggacca gttcttgacg
    32581 cgcaggtatt ccatcagcga tccgccgccg cgtcgcgttt gaagtcagcg cgaacgacgg
    32641 tgcgccgcgg cttctctggt tcgggctcct cgcgctgcgg caatggtccg tccatcgtga
    32701 ggaagcgcgt gaactctgga atgaacttga gataagccgt gccgacgggc ccgtctcgat
    32761 gcttacgcac gatcagctcg gcgatgccgc gccatggagt gtctgctcgg tagtagtcct
    32821 cccggtacac gaaggccacg atgtcggcgt cctgctcgat ctggcccgac tcgcgaaggt
    32881 cggcgagctg cgggcggttg tccgggcgcc cctcggccgc gcggttgagc tgcgcgacgg
    32941 cgatgatggg gatgttcaga tccttggcga tcgccttcag gccgcgggag atcgtcgcga
    33001 cttcctcgtg gcgcttctcg gccttggccg tcaaaagctg cagatagtcg atcaccgcca
    33061 gcgcgaggcc gtgcttgtgc ttcacccggc gcagcctcgc cctcacctgt ccgagcgtca
    33121 tggcgctgga gtcgtcgata tgcagcttga ggccgtatag atgatcaagg gcgccgtcgc
    33181 gcccaaggat cgagtcgtgg tagcgcagcg cgcgcccggc gatcttccgc ttcgtcatct
    33241 cgagcgacag gatggcgacg tgctcccgcg ctgcgacatg ctccgcgatg ttcatcgcga
    33301 gggcagtctt ccccatgctc gggcggccgg cgatcacgat aaagtcgcct ggcatcaggc
    33361 cgcgcaggat attgtcgacc gccgagtatc cggttgtaag gcctcgtccc gggttgtcgg
    33421 cccattcgac cgcctcgacc agcgcggcgc cgaactcggt ggcatcccca gaatcagcct
    33481 gagcctgcag cgcgagcagc gcggctgcgc cgtcgtccgc gatgacgagc ggctcctcgg
    33541 tccctgccat ggcgcgctcg gcggtctcca tggagcgctg cgcgatggag cgcaggagcg
    33601 cattcttctg cacgagctcg gcgtatcgga cgatgttgac cgccgacggc gtgttctgct
    33661 cgagggcacc gatgtacgca agcccaccga ccttttccag atcttcgccg agcgcatcgc
    33721 tgaccgtggt gaggtcggcc gcttttccag cctcgagcaa gcgcacgatg gcgctccaca
    33781 gacgacgatg ccgatcctga tagaacgccc cggcctcaag ccacgccacc ttgtcgaagg
    33841 cctggttgtc gaggagcagc gcgccaagcg tcgactgttc cgcctcggcg gaatgcggcg
    33901 gctcccttag attttcgaga actacttcgt ccgggtgcat tttgtagacc gctctctacc
    33961 cgttgtcgct caatgcccta gtgcctcggg cgaaaaaaag cgcggcagcg ccgcgcgttg
    34021 ttccctactt gaccgctttg atgtcttccc gcagcagacg ctcggccgcg cgcgtcatgt
    34081 tcttcacctc gtcggtgagc cgcgccatgt tctcggcgag gcggtcgcgc tcgacaaccg
    34141 gatcggctgg cagcgtcggc tgatagccga gctcggcgtc tatccaatgc ttcgcctggt
    34201 ggaagccggc atccttcgcc agcttcaaga ttttcatgaa ctcctcgatg cacagcttcc
    34261 gcggactctc gcggttgagg cagtcgagca attccttggt ggcctgctgg gcggccttgc
    34321 taggccagag gatctccgca accttcttcg cgccgccggc gtgctgtaca gcagccctgg
    34381 caacgtcgaa tacgtcctcg tagaagagct gctcttgggt catgcgggga atctttccca
    34441 agcgttccca aggcgctttt caaggaaaaa aaatacgctg cgcgtcatgc agccttttct
    34501 ttcagcccga gaagcgacac cacgtcgagc cgcgcgcgct tgtcggcgat cgcctcttcg
    34561 gaggctttgg tgagtttctt cgcgagatcc acggaggggc gccgatgccc gtaggcgatc
    34621 tggctgaagt attcaatgct ggtctcggcg gcctcgcaga cctgcctggt ggtctcgcga
    34681 ccgtgttttt ctaggaacgt cttcgcatcc atgcgcgcaa caatagcaac atgctactct
    34741 tccgtcaata gcactttgca cgttccatcg cgtagcaatt tgctatgcaa tgggcgggcg
    34801 atggaaaaca aggaagtacg gcggctcagg ctgctggagc tcgccaagca gtacggcacc
    34861 ctcaaggccc tggccgagga gaccggcacc gacccggcgc acctgagcca gatcaagaac
    34921 agcacgcggg acatgggcga ggacgtggcc cgccggatcg agcaggcgct caagaagccg
    34981 gcaggctgga tggatgcccc gcccggtgct cagggcccgc tagccgacga cgagctcgag
    35041 atgctcgaga agtaccgccg agcgtcgccg cggtggcgcc tggcgctacg ctacctcgct
    35101 gacgtgcctg acgaaagcca ggatgaggta tcggagagcg tgaacgttct gatggctaag
    35161 attttcgcaa agccggtgcc gaaccagcgc gtcgaggaag cctacggccg gccgccgact
    35221 cgggtatttt ctacacggga tcggaataaa acgtcactgc cggacgcgca gaaaaagcgc
    35281 agggcgtaga aacgaaaccg ataccggagg gctgtgcgct gacgcacaac ttcttcagag
    35341 aaactccttg ccccacgaac gtgcgaaaca tattgtgcgg cgtggggaaa attctgacac
    35401 tcgccgcccc gatgatctcc ctagagacgg tggcgacgtt ggtggatctc cttacggaag
    35461 cccaggccgg agaggtcatc gggatcgcct gcgtagttat ccacagggcc agccagttct
    35521 cgatcgacat cgccggggaa gcccggaaga cccccctcct cacccgcggc atggtcgccg
    35581 tcatggacga ccagctcgcc gtcctcatgg gctcgaagag gccctagccg ggcggttcca
    35641 cgtggaacta gcattttgct attgacagtg tagtagcagg ttgctattct ccttccaaca
    35701 gctcggcccc gccgggcgaa ggagggagag atggacccct acaatccgat catcggcgcc
    35761 gagcatgtcc agccggagcc gttctggaag ttcttcgccg ggatcgcgct cgccgcctgt
    35821 gccggcgtcg tggcgatgtg gttcgccacc gtcctcctct tcagctacga cgacgttctg
    35881 ctcgccgtgc tcgcgtccgt ggggctgctc tgatgggcgc cgtcttcgaa cgcgagctgg
    35941 acgtggaccc cgacgcctac ggcgacgaga agatgctcac cgaggctttc gacgccgccg
    36001 atgcgctgcg cgcgcatgag gaagccgctg cgaagcgcca gcccgcaccg atcccccagc
    36061 cgtgccacga atgcggccac cactacgcag gcatctcctg ccagatctgc aagaccgagc
    36121 ggccggcgtg gacggcactc aagaacatgg gcgcgatgag gaaggtcgct tgatccaagt
    36181 tttcaaagat cgagacctga cgccctgcat ggccgcctac gaaaagtggt ggaacgagca
    36241 gggcaagaac ctcggcatcc cgaccgaccg agcctggatc attttccgca atggctggtg
    36301 gatggccgaa gaatccaagg agaccgcatg agcgcagttc ccgcagtacg agagcagcac
    36361 gcacttgcaa cggccgactc gtcggccctg atgaagatca ttgagcgcgc gtcgaccgac
    36421 aaggatttcg acgttgcgaa gctggagaag ctgctcgagg taaaggaacg atgggaggcc
    36481 gccgaggcga agaaggcctt cgtagctgcg atggcggctt tcaaggccga gccgaccacc
    36541 atcctgaagc agaaggcggt caacatcccc ggcggcgcgc agttcaatca cgcgacgctt
    36601 gccgcagtgg tcgatggcgt cgtcgccaat ctcagcaagc acggcttctc gcatcgctgg
    36661 gaggtgatcc agcaggacaa gctcatcatt gtgtcctgca tcctgaccca cgagctcggg
    36721 caccaggagc gcaccacctt gagcggtgca cccgatgact cagggaagaa aaacgccatc
    36781 cagcaaatcg gctcgacggt gacttacctg cagcgctata cgctgatggc ggcaacgggc
    36841 ctcgccgcga aggacatgga caacgacggc ggcggcgctg ggcgcggtaa cggcggacag
    36901 atggatgaga aagcgctcgc cgatcatctc gccaacatcg acgcagcggt cgatcgcgaa
    36961 ggcctgatga aggctttcgg caccgcgtgg aatgccgccc aggacatgaa ggacaagggc
    37021 gcgcagcggc tcctgaacga gcacaagcaa gcccgcctca aggcgctggg ggctcgatga
    37081 agatcgtcga cttcccgcaa ggcaccccgg aatgggcggc ggcgcgcgcc ggcaaggtga
    37141 cggcctcctg catggacaag gtgctcgcca agatcaaggt cggcgaggcg gcggcccgcc
    37201 gggactaccg ggcgcagatc gtcgccgaga tcctgactgg cgcgccccag gacagcggtt
    37261 acatcaacgc cgagatgcaa tggggcatcg agcaggaacc cttcgcccgg ggggcctacg
    37321 aagcggctca atgcgttttg gtggacgagg tcggcttcgt catccatccg accctcgagc
    37381 gcgccggcgc gtcccctgat ggccttgtgg ggaccgatgg cctgatcgag atcaagtgcc
    37441 cgaagacggc gacgcacctg cagtacctgc tcgatggcgc ggtgccgaag gactatgagc
    37501 cgcagatgat gtggcaactc gcctgcaccg gccgcgcctg gtgcgacttc gtgagtttcg
    37561 acccgcggct gcccgagaag atgcagatgc tgcgcgttcg cctgcagcgc gacgacgcgc
    37621 gcatcgccga gctcgaagcc gaggtccgtg ggttccttct cgacgtggac cgcatgctcg
    37681 agacggtgcg gaggttcgcg tgatcggccg gctcatctgc ctcttcaagg gccacaagcg
    37741 cggcaagcgc gtgagcgaag cgaccagcaa cggcggtgca atgttcgccg acttccgctg
    37801 cccgcgctgt aaggcgacgt ggacgcgcaa ggcgaaggcg gtggctgcgt gatcgacgtt
    37861 ccccaggaca cgcccgagaa gccgctgact gcgatcgact acatcgcggc gctccaatcc
    37921 tgcgaggact cgctcgaggt tggcatcttc cgagaaaagg tgccgctcgc catcctgcac
    37981 gacaagcgtt tcaaggcggc cgtgcagtcc aagctctctg acatcgatag gcgaaaggca
    38041 gcatgagcgc cgcggcgaaa cgtcacctcg ccgagatcca cgagctcgcc tgcgtggttc
    38101 actggaattg ctacgggcag cgccggactg ccgacgaagc gcatcacctc gaatttgtgc
    38161 gcggagagca ttccgacttc gccacggttc cggtctgcgc tccatgccac gacgaactgc
    38221 acgaaagccg gcgacgggcg ttctaccgcg cgcacaagct ggacgacgtg aagctactgg
    38281 cctggacgat ccaggctctg caggcgcacc acgaactgtc gcggagattc gccgcgtgaa
    38341 cctcaagccc taccagctcg aacacggaga gaaattcggg aaactgaagg cgctgaactc
    38401 ctgcggcgtg aaaagtgagt gctggtgggt cgggtgtgac tgcgggagcc gcttcaaggt
    38461 gaagaagcgc catctcatca agggcaaggt gactgcgtgt ctaaagtgcg cgcctcctcg
    38521 tccgtagaag acgcggaact cttgcggaaa acgataggag caaacatgga ccacagcgaa
    38581 gtaaagcacc tcccgaaagt cgatcacacg ccggccgtgt tggacgatgc cagcaagctt
    38641 ttgctgaaga tggccgacgt gatcgaaacg cgaggccatt gccaacacct tgccgaggat
    38701 agcaaggggc gagtttgcat tgtcggcgcc accaattata cgggcaccat taagggatgg
    38761 tgcgaggtta tcgacaggat ggagagagcc atggggcaaa gattcattga gtggaataac
    38821 actccaggtc gcaccaagga agaagtcgtc gccaagctac gcgccattgc tttcctgcgc
    38881 gtctagcaaa tagcaacctg acaaatcaag gagaggtcat ggcgcaagcc aagaagaaag
    38941 cagcaaagaa gccgcaaccg aaactcacca tcgccaccgc ccgcaaggtg ctggaggtgg
    39001 tagacgccgg gctcgtcagt ggcatgggcg atccggttcc cggcaagatg tgcgtcgagg
    39061 ctgccgtctg cttcgccctc ggcctgccgc atggggataa cccgcaatgc gtcggctcgg
    39121 cagttcgcgc cttcaagatc cggctgaacg actcgcgctg gtctagcgat caggctcgcg
    39181 ccaaaggcat gcgagaggtg gccatcgcgc aactcggatc gaacgagatc gaccagaagg
    39241 agttcaagaa atacctcgcc atcggcatcg tccagaagat cgttccgatt gccatgcggg
    39301 cagcggccaa gatggtccct gcgcatgccg aggccctaga ggccgcagcg gtcgcgtgcg
    39361 aattggttct cgaccttccc tctgctcgcg cggcagcaag agcagctcaa gaaaaagcgc
    39421 tggcggcaag aaaggccgcc gccgcctacg ccgccgccta cgccgccg
//
LOCUS       DASVOR010000149         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_176799,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000149 DASVOR010000000
VERSION     DASVOR010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176799"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1379)
                     /locus_tag="VF516_03395"
     CDS             complement(54..1379)
                     /locus_tag="VF516_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106743.1"
                     /translation="MAEPGGELETLWPREDTAVGGQRRSTLPRRARGPLFAGVVVVTI
                     GAVVQVLALAWFELLTFLFRGSAEVLVHTGGLDRRTFAACSIAAFVLDLVLLAIIATR
                     SVRRSLRRAPPSDERFVARHPFAAASAGLLLALAFVIAGGWQPSPYFPYPLATVVVLA
                     TAYWFGLVGVLAMARSADLAWRAIRAWGVAAQWRTGFLLASLVLAGAAGYGLIATRWY
                     AAPLHAVEAGLALGSPGRAGDLLTGELDALCLVADRLEPSLAHSSAAPACEFLTTDSQ
                     PLDDCVAALIRDWVPSLKQQLRRRGLGPYVLDDAVMKALLAACTRQPPPRVRQAYFLA
                     MAENQATQASVPRAAACDATHPLAATCTADEPSESRARKLARLWDDALCRIGDDAAQV
                     LRRRLQQDESFREIADHLGIPEADARETYHHAIQQLRTPALASCDDAQY"
BASE COUNT          221 a          547 c          662 g          178 t
ORIGIN      
        1 gaggtcgagc ggctgtacga gcgcggccgg acctggctcg ccggcggcgg cctctagtac
       61 tgcgcgtcgt cgcagctcgc gagcgcgggg gtgcgcagct gctggatcgc gtggtggtag
      121 gtctcgcggg cgtcggcctc ggggatgccg aggtggtcgg cgatctcgcg gaagctctcg
      181 tcctgctgga gccggcggcg gaggacctgg gccgcgtcgt cgccgatccg gcacaacgcg
      241 tcgtcccaca gccgggcgag cttgcgcgcg cgggactcgg agggctcgtc ggcggtgcag
      301 gtcgcggcga gcgggtgggt cgcgtcgcag gccgccgcgc gcggaacgga ggcctgcgtg
      361 gcctggttct ccgccatcgc gaggaagtag gcctgccgca cgcggggtgg gggctggcgc
      421 gtgcaggcgg cgagcagggc cttcatgacc gcgtcgtcga ggacgtacgg gccgaggccg
      481 cggcgccgca gctgctgctt gaggctcggg acccagtccc ggatcagcgc ggcgacgcag
      541 tcgtcgagcg gctggctgtc ggtggtgagg aactcgcacg ccggcgcggc cgagctgtgg
      601 gcgaggctgg gctcgagccg gtcggcgacc aggcacaggg cgtcgagctc gccggtgagc
      661 aggtcgccgg cgcggccggg ggatccgagc gccaggccgg cctcgaccgc gtggagcggc
      721 gccgcgtacc accgcgtggc gatcagcccg tagccggcgg cgccggcgag caccaggctc
      781 gcgaggagga agccggtgcg ccactgggcg gcgacgcccc acgcccggat cgcacgccag
      841 gcgaggtcgg cggaccgggc catcgcgagg acgccgacga ggccgaacca gtacgcggtg
      901 gcgagcacga cgaccgtggc gagcgggtac gggaagtacg gcgacggctg ccagccgccc
      961 gcgatgacga acgcgagcgc cagcagcagg ccggcgctgg cggcggcgaa cggatggcgc
     1021 gcgacgaacc gctcgtcgga cggcggagct cgccgcagcg agcggcggac cgagcgggtc
     1081 gcgatgatgg ccagcagcac cagatcgagg acgaaggcgg cgatcgagca cgcggcgaac
     1141 gtgcggcggt cgaggccgcc ggtgtggacc agcacctcgg ccgagccgcg aaacaggaac
     1201 gtcaggagct cgaaccacgc gagcgcgagg acctggacca ccgcgccgat cgtgacgacg
     1261 acgacgccgg cgaacagcgg gccccgggct cgcctgggca gcgtcgatct gcgctgcccg
     1321 ccgacggcgg tgtcctcccg cggccacagc gtttccagct caccgcccgg ctctgccacc
     1381 tcaccaaatt actgctggta gccgcactcc aggtgccgcc aattgcttca cacgcgaagg
     1441 ccgtacggtg ccggcggaaa ccgcggaccc ggactggaag cgacggtgtg cgtgagggcg
     1501 cgctgcgggc tcgccggcgc ggttgtgatc gcgtcggcgc gtgccggcgg ccccgatcgc
     1561 cgtcgtgagc gtctgcgcga gcgcgagccg ggcgcggtgc gccaggcc
//
LOCUS       DASVOR010000150         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_469037,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000150 DASVOR010000000
VERSION     DASVOR010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469037"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(221..1303)
                     /gene="aroC"
                     /locus_tag="VF516_03400"
     CDS             complement(221..1303)
                     /gene="aroC"
                     /locus_tag="VF516_03400"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEX8106744.1"
                     /translation="MGSSFGALFRITTFGESHGPALGVVVDGCPPRLRLDLAQIQAEL
                     DRRRPGQSRLVSQRKEADRVEILSGVQGGVTLGTPIAMVIHNQDARSKDYDEIARAYR
                     PSHADYTYDAKYGIRAVAGGGRASARETAGRVAAGAIAQQLLAARGVTVVAWVDEVAG
                     IAAAVDEAAVGRDAVDATDIRCPDPAAAAAMIERIEAARKAGDSVGGVIRAIARGVPP
                     GWGEPVFDKLDADLAKAMMSIPAVKGVEIGSGFAGTRLTGSQHNDVFYRRDDGTIGTR
                     TNRSGGIQGGISNGEPVTVRIAFKPTATILQPQPTVDPAGNPTVLEPRGRHDPCVLPR
                     AVPIVEAMLALVLADHCLRQDARRGA"
     gene            1402..>1932
                     /locus_tag="VF516_03405"
     CDS             1402..>1932
                     /locus_tag="VF516_03405"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8106745.1"
                     /translation="MVDPDRRPTAQEIEELIDLVRRDPASPAFIDLGEAYLALGRPRD
                     ALQVGNVGLAATPDNLEGRVMLARAHVAMHQWKEAQGELLRVVKVDRGSRHGFALLGE
                     VLLRRQDFERAVPVLQHAQNLDPTSPQILAMLKRARAGQPLDPPPPIPTPVPPRGETD
                     NGHPILRSRNQPAAAVA"
BASE COUNT          247 a          721 c          711 g          253 t
ORIGIN      
        1 gggaaccgat gcgcgcgggc agcatgcccc cgcgcggcag gtcgctcgcc agccgccgcc
       61 ccgaggcgcc ggccgccccg gacagctcgg gcaggccgtc gtccggatcg tagccgagct
      121 cgtcgagcag ctgggcggac agcgaggcca tggcgcgacg ctatgccgcg ggtctgacac
      181 cggttccggc gccgccggga gtggcgcgag ctcactgggg ttacgcgccg cggcgcgcgt
      241 cctggcgcaa gcagtggtcg gccaggacca gggcgagcat cgcctcgacg atcggcaccg
      301 cgcgcggcag gacgcagggg tcgtggcggc cgcgcggctc gagcaccgtc gggttgccgg
      361 ccggatcgac ggtcggctgg ggctgcagga tcgtcgcggt cggcttgaac gcgatccgca
      421 cggtgaccgg ctcgccgttg gagatgccgc cctggatccc gcccgagcgg ttggtccgcg
      481 tgccgatcgt gccgtcgtcg cgccggtaga acacgtcgtt gtgctggctg cccgtgaggc
      541 gggtgccggc gaagcccgag ccgatctcga cgcccttgac cgcggggatc gacatcatcg
      601 ccttggcgag gtcggcgtcg agcttgtcga acaccggctc gccccagccg ggcggcacgc
      661 cgcgggcgat cgcgcggatc acgccgccga ccgagtcgcc ggccttgcgg gcggcctcga
      721 tccgctcgat catcgccgcg gccgccgccg gatcggggca ccggatgtcg gtggcgtcga
      781 ccgcgtcgcg cccgaccgcg gcctcgtcga ccgccgctgc gatcccggcg acctcgtcga
      841 cccatgcgac gacggtcaca ccgcgcgccg cgagcagctg ctgcgcgatc gcaccggcgg
      901 cgacccggcc cgcggtctct cgcgcgctgg cccgaccgcc gcccgcgacc gcgcggatcc
      961 cgtacttggc gtcgtaggtg taatcggcgt ggctcgggcg ataggcccgc gcgatctcgt
     1021 cgtagtcctt gctgcgcgcg tcctggttgt gaatcaccat cgcgatcggc gtgccgagcg
     1081 tgacgccgcc ctgcacgccg gacaggatct cgacgcgatc ggcctccttg cgctggctga
     1141 ccagccggct ctgcccgggg cggcggcgat cgagctcggc ctggatctgc gcgagatcga
     1201 gccggagccg cggcgggcaa ccgtcgacca ccacgccgag cgcgggtcca tgcgattcac
     1261 cgaacgtggt gatccggaac agcgcgccga agctggagcc catcgcgcgc agcatacgcg
     1321 accgcgacgc gaccgggtcg tcgggttgtc accatgtagg tttgccgggc agggcggttt
     1381 ggaggtacgc tcgatgagct gatggtcgac ccggatcgcc ggcccacggc tcaagaaatc
     1441 gaggagctga tcgatctcgt gcgccgcgac cccgcgtcgc ccgcgttcat cgatctcggc
     1501 gaagcctacc tcgcgctcgg ccggccgcgc gacgccctgc aggtcggcaa cgtcgggctg
     1561 gcggccacgc ccgacaacct cgagggccgg gtcatgctgg cgcgcgccca cgtggcgatg
     1621 caccagtgga aggaggcgca gggcgagctg ctccgcgtgg tcaaggtcga ccgcggcagc
     1681 cgccatggct tcgcgctgct cggcgaggtc ctgctgcgcc gccaggactt cgagcgcgcg
     1741 gtgccggtgc tgcagcacgc ccagaacctc gacccgacca gcccgcagat cctcgcgatg
     1801 ctcaagcgcg cgcgggccgg ccagccgctc gatccgccgc cgccgatccc gacgccggtg
     1861 ccgccgcgcg gcgagaccga caacggccac ccgatcctcc gctcgcgcaa ccagcccgcg
     1921 gccgcggtcg cg
//
LOCUS       DASVOR010000151         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_118382,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000151 DASVOR010000000
VERSION     DASVOR010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118382"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(288..1031)
                     /locus_tag="VF516_03410"
     CDS             complement(288..1031)
                     /locus_tag="VF516_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106746.1"
                     /translation="MISGASTTLTSTGGVAVAHGEIFVSDPGANAIDVFPVSANGDVA
                     PIRRIAGGSTRLAVPTGLVVADDQLFVAQKQGPILMFPIDASGDVAPTRSYPVVDEAD
                     HALAVDSGELYVSNVGASIRVYPVVGADVTGPTRIIAGPSTGLIDSAGIVVHNGEIIV
                     TCPTANRILVFPEQADGDVPPLRTIIGPDTELGSPTGLALLHDELYVLSLFLRSVQVL
                     PVASAGDVPPTRTIENAGVLTRPIAVAVF"
     gene            1258..>1616
                     /locus_tag="VF516_03415"
     CDS             1258..>1616
                     /locus_tag="VF516_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8106747.1"
                     /translation="MPISGMNHFTVLTSDLDASKAFYVGLLGLTEGFRPDLGFPGAWL
                     YAGDQAVLHIVAGRGVPASPRGVIDHMAFSARDLRAVAARLTERGIAYDLKRLPSTGA
                     WQLFCLDPSGARVELDF"
BASE COUNT          242 a          595 c          556 g          223 t
ORIGIN      
        1 ccccagcgcg cgccgctggt ggcggcggac cagctcgtcg aacgctgcgc cgcggccgcc
       61 gcgggcaagc agcatgagct cgtcgtcgcc gagctcgctc aacgggcgct ccccggggtc
      121 ggttgtcccg gcgatcacga gcggaaccac ggttcccgcg gccatccccg ggagaatgcg
      181 cgacccgcac aaaggttcgg tcccgcgccg cacgctgcga tccgacctgg acatccgtgc
      241 agatccgccg gggcccgggg cccgaccgca gcgtacggcc acagcaccta gaacacggcg
      301 acggcgatcg gcctggtgag cacgcccgcg ttctcgatcg tccgggtcgg tggcacgtcg
      361 cccgcggacg cgacaggaag cacctggacc gagcggagga acaggctgag cacgtagagc
      421 tcgtcgtgca acagcgccag ccccgtgggc gagccgagct cggtgtcggg cccgatgatc
      481 gtgcgcagcg gcgggacatc gccgtccgcc tgctccggga acacgaggat ccggttggcg
      541 gtggggcacg tgacgatgat ctcgccgttg tggacgacga tccccgcgct gtcgatcagc
      601 ccggtcgacg gtccagcgat gatccgggtt ggcccggtca cgtcagcgcc gaccaccggg
      661 tagacccgga tcgacgcgcc gacgttggac acgtagagct cgccgctgtc gaccgccagg
      721 gcatggtctg cttcgtcgac cactggatag ctccgggtcg gcgcgacgtc gccggacgcg
      781 tcgatcggga acatcaggat cgggccctgc ttctgggcca cgaacagctg gtcatcggcc
      841 accaccagcc cggtcggcac cgcgagccgg gtcgaaccgc cggcgatcct gcggatcggc
      901 gcgacgtcgc cgttcgcgct gaccgggaac acgtcgatcg cgttggcgcc gggatcggag
      961 acgaagatct cgccgtgcgc caccgcgacg ccgcccgtgc tggtgagcgt ggtcgacgca
     1021 ccgctgatca cgctccgagc tgcgcccgta cgcagtaccc cttcagggcg agcagaccaa
     1081 gatactccac accatgatca cgcaactcac gactctcatg acatctcctt gatcgagcga
     1141 gcccgggcac cactgcccgc ggcgcttgcc gcacgagagt gcgtgccggc ccggaaagtt
     1201 cggccggcgc ggcgcgcacc agcgggcgac gccggcttcg cgtaagctgt cctcgccatg
     1261 ccgatcagcg ggatgaacca cttcacggtc ctgaccagcg acctcgacgc ctcgaaggcg
     1321 ttctacgtcg gactcctggg cctcaccgag ggcttccggc ccgacctcgg cttccccggc
     1381 gcgtggctgt acgccggcga ccaggccgtg ctgcacatcg tcgccgggcg cggcgtgccc
     1441 gccagcccgc gcggcgtgat cgatcacatg gcgttcagcg cgcgcgacct gcgcgccgtc
     1501 gccgcgcggc tgaccgagcg cggcatcgcc tacgacctga agcggctgcc gtcgaccggc
     1561 gcctggcagc tgttctgcct cgatccgagc ggcgcgcgcg tcgagctcga cttcga
//
LOCUS       DASVOR010000152         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_176808,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000152 DASVOR010000000
VERSION     DASVOR010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176808"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="VF516_03420"
     CDS             <1..274
                     /locus_tag="VF516_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106748.1"
                     /translation="DDESLGSYACVHIQPVGADDVKLFRLLDDDTRCILQIRGGPSNV
                     NNDSIAACAKVARDTIGPAQIKVNAAAGSAGRASGLRIRSSRSSGR"
     gene            complement(246..593)
                     /locus_tag="VF516_03425"
     CDS             complement(246..593)
                     /locus_tag="VF516_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106749.1"
                     /translation="MRFLPSLVVCALIGLALPARADTTATSSIAQLMVLEPGDTSYKL
                     FHGAVWLDHDKAQWNYRWGGLQCKGRELSDNSVQLLFAAFRSEYQVTLEYVVTDYKGK
                     PYRCITGLTISKT"
     gene            complement(685..>2001)
                     /locus_tag="VF516_03430"
     CDS             complement(685..>2001)
                     /locus_tag="VF516_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106750.1"
                     /translation="SGCVVTMGNNDARTQVSSIPNPSPARPGPTFTISAYQAAPGVPV
                     DMEWGQLVKCMQEVYSPYNLTVTDAQPPAGTPYNEAIIAGTPDQIGQDTNVLGVAPLA
                     SDCSAIGNVISFSFANAHGTIDRVNNICWTAAQESAHAYGLDHEYTFTTGNAANNHSA
                     CNDPMTYRNDCGGEKFFRNETANCGETANRACKCGGTQNSHLKVLAVFGASTAAPIVA
                     RPTAAIVSPAAADTMLSFSVVINAGAQRGVARVELYFNGFKWGEAPGAMFGQRGQPNP
                     SPYSIAVPTALPNSIVDVKAIAYDDLGASTESAVVTVTKGAPCTAATTCAKGQKCDAG
                     KCLWDPPSGEIGASCSYPQFCKSGLCTGTAEQQICTQSCILGTTDSCPPSFDCVANGP
                     STGVCFFSSTGGCCSVDRGGGVWWLPVGLGLAVLGLVARPRRRRRR"
BASE COUNT          288 a          680 c          724 g          309 t
ORIGIN      
        1 cgacgacgag tcgctcggca gctacgcctg cgtccacatc cagccggtcg gcgccgacga
       61 cgtcaagctg ttccggctgc tcgacgacga cacccgctgc atcctccaga tccgcggcgg
      121 cccgagcaac gtcaacaacg acagcatcgc cgcctgcgcc aaggtcgcgc gcgacacgat
      181 cggcccggcg cagatcaagg tcaacgccgc cgccggctcg gccggccgag cgtcgggtct
      241 ccggatcagg tcttcgagat cgtcaggccg gtgatgcagc ggtacggctt gcccttgtaa
      301 tcggtgacca cgtactccag ggtgacctgg tactcggagc ggaacgcggc gaacagcagc
      361 tggacgctgt tgtccgacag ctcgcggccc ttgcactgca gcccgcccca gcggtagttc
      421 cactgcgcct tgtcgtgatc gagccacacc gcgccgtgga acagcttgta gctggtgtcg
      481 cccggctcga gcaccatgag ctgggcgatc gacgaggtgg cggtggtgtc ggcgcgcgcc
      541 gggagcgcca ggccgatgag cgcgcagacc acgaggctcg gcagaaaacg catcgccgct
      601 atcgtcgtcc cgagcgggat cgcgatgcaa gcccgaccgc cgccccgaat cgacgcggcc
      661 cggatcggcg caaccggccc gggttcagcg ccgccggcgg cgccggggac gtgcgaccag
      721 cccgagcacg gccaggccga ggccgaccgg cagccaccat acgccgccgc cgcggtcgac
      781 gctgcagcag ccgccggtcg acgagaagaa gcacacgccg gtgctgggcc cgttcgccac
      841 gcagtcgaag ctgggcgggc agctgtcggt cgtgcccagg atgcagctct gcgtgcagat
      901 ctgctgctcg gcggtgcccg tgcagagccc cgacttgcag aactgggggt acgagcagga
      961 agcgccgatc tcgccgctcg gcggatccca caggcacttg ccggcgtcgc acttctggcc
     1021 cttggcgcag gtcgtcgcgg ccgtgcacgg cgcgcccttg gtgacggtga ccacggccga
     1081 ctcggtcgag gcgccgaggt cgtcgtaggc gatcgccttg acgtcgacga tgctgttggg
     1141 cagggcggtc gggaccgcga tgctgtaggg gctcgggttg ggctggccgc gctgcccgaa
     1201 catggcgccc ggcgcctcgc cccacttgaa gccgttgaag tagagctcga cgcgcgccac
     1261 gccgcgctgt gcgccggcat tgatcaccac cgagaagctg agcatggtgt cggccgcagc
     1321 cggcgagacg atcgccgccg tcggccgggc gacgatcggc gcggcggtgc tcgcgccgaa
     1381 caccgccagc accttgaggt gcgagttctg cgtcccgccg cacttgcacg cccggttggc
     1441 ggtctcgccg cagttggcgg tctcgttgcg gaagaacttc tccccgccgc agtcgttgcg
     1501 gtaggtcatc gggtcgttgc aggccgagtg gttgttggcc gcgttcccgg tggtgaacgt
     1561 gtactcgtga tccaggccgt aggcgtgcgc gctctcctgc gccgcggtcc agcagatgtt
     1621 gttgacgcga tcgatggtgc cgtgcgcgtt ggcgaacgag aacgagatca cgttgccgat
     1681 cgcgctgcag tcgctggcga gcggcgcgac gccgagcacg ttggtgtcct ggccgatctg
     1741 gtcgggggta cccgcgatga tcgcctcgtt gtagggggtg ccggcgggcg gctgggcgtc
     1801 ggtgacggtg aggttgtacg gcgagtacac ctcctgcatg cacttgacga gctgccccca
     1861 ctccatgtcg acgggcaccc cgggagcggc ctggtacgcg ctgatcgtga acgtcggacc
     1921 cggccgggcc ggcgacgggt tcgggatcga cgacacctgg gtgcgcgcgt cgttgttgcc
     1981 catggtgacg acgcagccgc t
//
LOCUS       DASVOR010000153         3773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_59843,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000153 DASVOR010000000
VERSION     DASVOR010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3773
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59843"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VF516_03435"
     CDS             <1..411
                     /locus_tag="VF516_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106751.1"
                     /translation="PDRLTVAALGDAAAGHLRDYLRAVPSFTLFFVPEGLAFIDHVAA
                     LPVPELVHSVGRFERAMWLARFEPQRPPTVIEFAAPPDLVLGAALQGQPLPAPSAELH
                     FIVVDPGVAGRWRIATADDVAALERSTTTWPPSP"
     gene            complement(389..832)
                     /locus_tag="VF516_03440"
     CDS             complement(389..832)
                     /locus_tag="VF516_03440"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8106752.1"
                     /translation="MSNVSQTIQRLEPTPPGHPGLPRIHQVVIHGDVVYLAGITLLQA
                     LPVAALLALAQPGGVAAANFGDVADQTRRICGRIDELLATAGTSKAKLLTAQVWLTDM
                     KHFAAHNDAWNEWVDPDNAPVRACVVSPQLVLPGLLVEIMVTAAR"
     gene            complement(1022..1420)
                     /locus_tag="VF516_03445"
     CDS             complement(1022..1420)
                     /locus_tag="VF516_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106753.1"
                     /translation="MADVQDAGRFGDRKVFIASLWTRMVTIEAQTGGTLTEGATIEHF
                     KAWLLRSRLLTCDGTEHGARLIVLCRADLVAAMDPALVAASETTTHGAVFHFALDRQV
                     ACTDYAPRKATVHSATTGKVGRVGASRRAA"
     gene            complement(2032..2217)
                     /locus_tag="VF516_03450"
     CDS             complement(2032..2217)
                     /locus_tag="VF516_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106754.1"
                     /translation="MTKTVQVGFRLPADLVKRIDAYAARIANENPGLEITRVVAVKAL
                     LTQALDAVEATPKHRGR"
     gene            2224..2400
                     /locus_tag="VF516_03455"
     CDS             2224..2400
                     /locus_tag="VF516_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106755.1"
                     /translation="MVILAIRVAAKVRRFSIRTPLVTGWIDGAVMDMLIYGNEIPIPE
                     EFASTGLEDDGSDG"
     gene            2397..2804
                     /locus_tag="VF516_03460"
     CDS             2397..2804
                     /locus_tag="VF516_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106756.1"
                     /translation="MIRVILYRVGVKPQVISVSPSDDGNHLAALQQTLGGPVACVPLH
                     DGIQLYCNRGGLLFGLALARRALALSLAEPWRFEVRLHFENSERDLGPKEWPANGDFV
                     LARVGACGELMDLTEDDIRLWMFWLGLDHILNR"
     gene            3091..3222
                     /locus_tag="VF516_03465"
     CDS             3091..3222
                     /locus_tag="VF516_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106757.1"
                     /translation="MVGIEPAAPTAVIRLPQGIVIELASASPQWVAALARELTRPAT"
     gene            complement(3412..>3773)
                     /locus_tag="VF516_03470"
     CDS             complement(3412..>3773)
                     /locus_tag="VF516_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106758.1"
                     /translation="WSRCDLAFEDFLWRGNLRPVVLLSSAAAATLAVRAMWIVAPAND
                     SRGIVELSVGEKEGLSRAGVLRVAVPQPDRILCDWLTTLDSAELVERAGALSREKLVQ
                     LDEMLRLAQLDPERWAP"
BASE COUNT          544 a         1299 c         1284 g          646 t
ORIGIN      
        1 cccgaccgcc tgaccgtcgc cgcgctcggc gacgccgccg ccggccacct ccgcgactac
       61 ctccgcgcgg tgccgagctt cacgctgttc ttcgtccccg agggcctcgc cttcatcgac
      121 cacgtcgcgg cgctcccggt cccggagctc gtccactcgg tcgggcgctt cgagcgcgcc
      181 atgtggctcg cgcggttcga gccgcagcgc ccgccgaccg tcatcgagtt cgccgcaccg
      241 ccggacctcg tgctcggtgc cgcgctccag ggccagccgc tgccggcgcc gagcgccgag
      301 ctgcacttca tcgtggtcga ccccggggtc gccgggcgct ggcgcatcgc gaccgccgac
      361 gacgtcgccg cgctggagcg gtcgacgact acctggccgc cgtcaccatg atctccacca
      421 gcaggccggg gagaaccagc tgcggcgaca cgacgcacgc gcgcaccggc gcgttgtctg
      481 gatcgaccca ttcgttccat gcatcgttgt gggccgcaaa gtgcttcatg tcggtcagcc
      541 acacctgtgc cgtcagcagc ttcgccttcg aggtgccggc ggtcgccagc agctcgtcga
      601 tccgcccgca gatccgccgc gtctgatcgg ccacgtcgcc gaagttggcg gcggcgacgc
      661 cgcccggctg ggcaagcgcc aacaacgccg cgacgggaag cgcctggagg agcgtgatgc
      721 ccgccagata gacgacgtcg ccgtggatga cgacctggtg gatccggggc aagccggggt
      781 gccccggcgg cgtcggttct aagcgctgga tcgtctggct gacgttcgac atgcgtgtct
      841 ccgagaagtt ctgacggatc caataccatg ggcgaccgcg cgagccatcc gagagcgcgg
      901 cacgatcgtc cgccggccgc tcgcggtccg ccgaccgctc tggccagcgg cgcgctcgcg
      961 gtcggcggcg cgctcgcggt ccgcctggca tacgcggtcg gccaggcgcg ctcgcggtcg
     1021 gctacgcggc tcgccgcgag gctcccaccc tccctacctt acctgtcgtg gcgctgtgca
     1081 ctgtcgcctt gcgaggcgcg tagtcggtgc acgccacctg ccgatcgagc gcgaagtgaa
     1141 acaccgctcc gtgtgtggtg gtctcgctcg cagcgaccag cgcaggatcc atcgcggcaa
     1201 cgagatcggc gcggcacagc acgatcagcc gcgcgccgtg ctcggtgcca tcgcacgtga
     1261 gcaaccggct gcggaggagc cacgccttga agtgctcgat cgtcgctccc tcggtcaggg
     1321 tgccgccggt ctgggcctcg atggtaacca tccgggtcca gagcgaggcg atgaacacct
     1381 tgcggtcacc gaagcggcct gcgtcctgga cgtcggccat agcgcggtgc aagaggcggg
     1441 cgatctcgag gtcgttgtgg gttggggttc ggtcgatacg agcggtggcc gtatgggtca
     1501 tgccctgcag tcaatgcgga ttatgtgcca gtgccaccga ttggctatct tcttatattg
     1561 tggataatcg gattatgtat ctctggcgga tgtgttgttg tgtggcaaca cctgtgtgtc
     1621 tgcccctcac ggtcgaagcg ccgcagtcgg cgcgttcgcg gtcggcgcct ttcgcggtcg
     1681 gtccacccgc ggtcggccag cgcgtccctt catgatctgg tcgtcggatc cggcgccgcc
     1741 tgcgtcgcgg ccctcgtcga ccgattcaac cagcactgcc acagctggtg atcacggccg
     1801 actcgtggcg cgcacgcggc ggtagcctcg tcgaccgtca gatcacgacg gaaactcgct
     1861 gccgtttaca gaccttgacc ttgcactcgc tgccgatggt ctcgtccccc gcgcttgatc
     1921 gcacccgccg gccccgcgga cgtgtacgcc gcgctgtggc tcggagtgct cctcgccgcg
     1981 agcgggaccg ttcgacgcag ccggtatctg ccccccgtcc cgccggggct cctacctgcc
     2041 gcggtgcttc ggcgtggctt cgaccgcgtc gagcgcctgg gtgagcagtg ccttcaccgc
     2101 gaccacgcgc gtgatctcga ggccggggtt ctcgttcgcg atccgcgccg cgtacgcatc
     2161 gatccgcttc acgagatccg ccgggagtcg gaagcctact tgcaccgtct tcgtcatgcg
     2221 gccttggtca tcctggcgat ccgggtcgcc gcgaaggttc gccggtttag catccgcacg
     2281 ccactggtga caggctggat cgacggggca gtcatggaca tgctgatcta cgggaacgag
     2341 ataccgatcc cggaggagtt tgcatcgacg gggctcgagg acgacgggag cgacggatga
     2401 tccgggtcat cctgtaccgg gttggggtaa agccccaagt gatctcggtc tcgccgagcg
     2461 acgacgggaa ccacctggcc gccctgcagc agacccttgg cgggccggtc gcgtgcgttc
     2521 cgctgcacga cggtatccag ctctactgca accgcggcgg gctgctgttc ggcctcgcgc
     2581 tcgctcgccg cgcgctggcg ctgtccctcg ccgagccgtg gcggttcgag gtgaggctcc
     2641 acttcgagaa ctcggagcgc gacctcggcc cgaaggagtg gcctgccaac ggagacttcg
     2701 tgctagcccg ggtcggcgct tgcggcgagc tgatggatct gaccgaggac gacatcaggc
     2761 tgtggatgtt ctggctcggg ctcgatcaca tcctgaatcg ctgaacggtc gctggggctc
     2821 gccggcgagc gcggggcggg cagcgctggc ggttaaaccg ggcacccggc tcgaaccgct
     2881 cgcgctcgca ttgaacgcgt acgcttgccg atgagcgagc cgctccgatc accggtcggg
     2941 cgaaggttcc gccgaggaga tctgtgttgc ggcgaaacac tcgaggcatt tgcccgatca
     3001 cgcggactgg ttccacagcg gctggcgtgg tggcggaaac ggctgagcgc gagtccggca
     3061 ggtccagcat tgacgttggt cccggcggcc gtggtcggca tcgagccggc ggcgccgacg
     3121 gcggtgatcc ggctgccaca ggggatcgtg atcgagctgg ccagtgcgtc gccacagtgg
     3181 gtcgcggcgc tggcgcgcga gctgacgagg ccggcgacgt gatcctcttg cctcgtgccg
     3241 tgcgggtgta cttccgatcg cgccgaggaa gaagcgctcg agatcgaccc gttcgggatc
     3301 ccacttgagc gttccgtcga gcgtcttgac gatcgctgct tccggccgct gcgcggcgtg
     3361 atgatgcttg acgcgccatc gacaccgtat cgaggacggc tcaggatgcg atcaaggggc
     3421 ccagcgctcg ggatccaact gagccagccg aagcatctcg tcgagctgca ctagtttctc
     3481 gcgtgagagc gcacccgcgc gttcaaccag ctcggcgctg tccaaggtcg taagccagtc
     3541 gcaaaggatg cggtcgggct gaggaaccgc aacgcgaagt acgcctgcac gcgagagccc
     3601 ctccttttcg cccaccgaca gttcgacgat gccgcggcta tcgttcgccg gagccacgat
     3661 ccacatcgct cgaaccgcga gcgttgctgc tgccgccgac gaaagcagca ccactgggcg
     3721 gaggttccct cgccagagga agtcctcgaa ggcgagatca catcgactcc acc
//
LOCUS       DASVOR010000154        11114 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_469041,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000154 DASVOR010000000
VERSION     DASVOR010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11114
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469041"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..2612
                     /gene="bamA"
                     /locus_tag="VF516_03475"
     CDS             279..2612
                     /gene="bamA"
                     /locus_tag="VF516_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX8106759.1"
                     /translation="MKRSWLVVVTCLWFASTAHAQDIERVEVRGNRRVEAETIRAAVA
                     TRPGQALEPARLRSDLRAIWKLGMFSDVRAVTDGGVLAFEVVERPAIRKILVAGNHEL
                     ALDKIDEVIELKRGAIADEVAIRKSRDKIADLYLEHGFMFATVDSAVLPVDSGEVDVR
                     FTVDEHAKVMVSDIAFTGNRAVASGELRKLMATSPPGALSFITGSGVFRRDELERDLA
                     ILTGYYLDHGFATVKIAPPVLDPSRDRKRLLVTIAIEEGPRFTYGAIDVKGDLLGTPA
                     QHLALIHSRPGATFVRSMVEADRKALEVHYQDLGFAHASAVPRPRVDFAHHRIAIDFE
                     IVRGKRVFVERIAVRGNSKTRDKVIRRELQIAEGELFSATNLERSRQRVLALGYFEAV
                     TIATRRGSSDDMIEVELEVRERSTGTFQVGAGFSSTESFLVQGQVAMDNVLGRGQSLS
                     GQVQLSSVRRLFALHFVEPYVLDSRWTLGTELYNQTSSLGAYARTSTGGSLTLGYPLA
                     DHLHGFLTYRLEDVGITTGFGGFAGLGATAVQLPALDTANLFRGGWTSSIRGALAYDT
                     RDNRLVPTHGAYATAYAEYAGRPTGSENEFVRYGGFARRYRPLGPFVLRLNAEFGVTR
                     SLDGRGVPLTERYLLGGISDIRGYQPRSLGPHLWSQHPGDVGQPLDPLAIGGNLELIG
                     NAELEFPLVKSIGLSGVAFFDIGNAYNLESRFCTRPGSDACTRGFDAITSLRKSVGLG
                     VRWRSPIGPLRLEWGLPLDLKRGEKPDGLEFSIGTSF"
     gene            2713..3045
                     /locus_tag="VF516_03480"
     CDS             2713..3045
                     /locus_tag="VF516_03480"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8106760.1"
                     /translation="MPERRDTDLLFKALADPSRRKLLDLLHAHDGRTLTELCEHLDMT
                     RQGVTQHLDLLEAANLVVTVRRGREKLHFLNPVPLQELYERWIAKFEKPRLKALEDLK
                     RRLEKANG"
     gene            3062..3493
                     /locus_tag="VF516_03485"
     CDS             3062..3493
                     /locus_tag="VF516_03485"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8106761.1"
                     /translation="MTYIRTTPEKLWSALTHDVEFMKQYWFGNHCDSQWTAGSPWKNV
                     SPQGVVLDAGEIIEADPPRRLVIRWHHQHKPELEAEGASRCTMELEPSGAAVKLSITH
                     TIEREPSKLIEAVSGGWPKVLSNLKSLLETGSIALQEPYPH"
     gene            3490..3861
                     /locus_tag="VF516_03490"
     CDS             3490..3861
                     /locus_tag="VF516_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106762.1"
                     /translation="MRAPGYSGRGIDATLRVTDATNCSAEPVACTASTTACVPVPLRL
                     GWLAVPGPFGFPSSFAITLSADMTALYPSGATPTITWTAGCPLSVATSNLSATMPAPG
                     CKVTVTLHTNNKTFAVSAVPN"
     gene            4343..4558
                     /locus_tag="VF516_03495"
     CDS             4343..4558
                     /locus_tag="VF516_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106763.1"
                     /translation="MDELLAWTGGGHQRYRHEGPDVREVRERDIQHDRERGELHDLDK
                     LPAWRLRERKWDIGFRSPVRAMRGLLR"
     gene            complement(4722..5912)
                     /gene="tgt"
                     /locus_tag="VF516_03500"
     CDS             complement(4722..5912)
                     /gene="tgt"
                     /locus_tag="VF516_03500"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEX8106764.1"
                     /translation="MRLEIHATATGSRARVATLVTSHDRVETPAFMPVGTRATVRTQT
                     LAQLEQLGAPILLANTYHLLVRPGLDVFERFGGLHRWMGWTHSILTDSGGFQAFSLPC
                     TISEAGAELHGLDGRSLLLSPEQSIAAQRAIGSDIMMALDHCIASTSSRAETEAAMHR
                     THRWANRSLAAHRAAPPAPDLDAPPAALFAIVQGACFPDLRRTSAEVLTNTDGFDGYA
                     IGGLAVGETRSQREDITELVTELLPADRPRYLMGVGTPLDLLEGVHRGVDMFDCILPT
                     ALAQQGVAFTSHGRLDLERGVYKLADQPLDRACACDACMRHSRSYLHHLMKCQEPLGW
                     QLLAQHNLVFYVRLMAAMRAHIRADTFAAFYREQRELLARADPDHPPGPRPRVKRRKA
                     QVQR"
     gene            6299..7228
                     /locus_tag="VF516_03505"
     CDS             6299..7228
                     /locus_tag="VF516_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106765.1"
                     /translation="MATTTTTTTGPGPEPAPPLRSELVEGVRTTSNARWGAIIGGAFM
                     AVASWIVLHLLGLGVGLTVIDPNQTSSLRAIGLGTGIWSLIAPIIAVFIGGLVVSRLA
                     PTPSRLTRVIHGGLVWAVTTLATITLLVALASSLVRGAVSTGGQVVSTAAGAVGGVAG
                     AVDRDALSSLGIKSDDLLAPINQRLRAEGKSEVTAQQLEAALKDTLRTAVRTGNLDRQ
                     TLVNALATNTPLTPRDADQIANSVEARWQQVQQRGSELANRASTAALEAAQATGKALL
                     GLAIALILGLVAAAGGSLLTGNIDRRRPRPASE"
     gene            7366..7929
                     /locus_tag="VF516_03510"
     CDS             7366..7929
                     /locus_tag="VF516_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106766.1"
                     /translation="MRMWLVAGVTVVGCGGCSSSKSLDCAYLAGNNCWKTTVAEATTC
                     LPAAGTTGKLSADNSSCAYASGQTITFTPALGLPLASGFKDWNFTVTTGGQPCLHYEE
                     SAGGFELTVRADAVSEMISGAAGIDLTCPDGTTYSNSNALTLLDCPGSAFGDLPGNTT
                     SSDSTSVTFGLINTGTPSSVTVFDCSR"
     gene            complement(7994..8839)
                     /locus_tag="VF516_03515"
     CDS             complement(7994..8839)
                     /locus_tag="VF516_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8106767.1"
                     /translation="MTGEPMEKKIEMLNVPGARLYTETRGSGPLLVCIVGGNGDAESF
                     GRLAALLARQFTVVAYDRRGYARSPLDAPVDDAARLALDADDAMRVIERHGGPAYVLG
                     SSSGAIVGLDLLARSPGCIRKLVAHEPPLVTLLPDAAHWLAMFEHVHATYLASGVEAA
                     MAEFGPAVGMPVLRPPPSMQLSPEIAAMLARLPENRAFWLARELRTYPRFAPDLAALR
                     AAAGQLVLAVGREPPDAPLARPARVLAASLGVRLVEFVGGHVGYAVDPVAFAAELAPL
                     LTASP"
     gene            complement(9226..9504)
                     /locus_tag="VF516_03520"
     CDS             complement(9226..9504)
                     /locus_tag="VF516_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106768.1"
                     /translation="MSPWLAAAEGPGGQLGRALDRPPFGIAPMMVDVVDQRHLESLAD
                     RQQILDGVHPIVVLQHDPRTRPRRQARQPRGQAFEARALAAVRAAPGA"
     gene            9921..>11114
                     /locus_tag="VF516_03525"
     CDS             9921..>11114
                     /locus_tag="VF516_03525"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8106769.1"
                     /translation="MPFPPAHPRLLSALADRQYLEPTPVQAAVLADEARDRDLLVSAQ
                     TGSGKTVAFGLALAHTLLGDAPRFSPGGAPRALIVTPTRELALQVQRELVWLYAETGA
                     RIASCVGGMDPRREQHVLRQGVQIVVGTPGRLCDHLDRGALVLSGLQAIVLDEADEML
                     DMGFREDLERLLEAAPTERRTLLFSATIPREIAALAKRYQKHAMRIATSTEAGRHHDI
                     EYHAIPIVPRERDLAVVNVLRYHEAAGALVFCATRDGVAHLAANLAERGFAVVALSGE
                     FSQRERNTALQALRDGRARVCVATDVAARGLDLPDLGLVIHADLPQNQQVLVHRSGRT
                     GRAGRHGVAILLVPDPARRYVERMLRAAHVDATWSRAPTADEILARDQELVIGEIESL
                     TEELSD"
BASE COUNT         1695 a         3897 c         3895 g         1627 t
ORIGIN      
        1 cagcgcgacg ccgctgacgc ccgtcgcgac gcgcgcgcgt ccgtgacgcg cgggacgggc
       61 cgcgcgcgcg cgttctccgg cggggcgtag catcctgcac aaaaaaacgg cacgcacgcg
      121 tcgtgccatg cgcgcgatcg cgaggcaacc gcgcgcgagg tcgccgggtc ggactcgaca
      181 acactgctgc cgccgagcgc tccggccgag gaggccgacg gctcggattc atgacacggg
      241 gtgctccgtc acgcgcgccg cgtcgaggag ctggacgttt gaagcgctcg tggctggtcg
      301 tcgtcacatg cctgtggttt gcaagtaccg cgcacgcaca ggacatcgaa cgcgtcgagg
      361 ttcgcggcaa ccgtcgcgtc gaggcggaga cgatccgcgc cgccgtcgcg accaggcccg
      421 ggcaggcgct cgagccggcg cggctgcgca gcgacctgcg cgcgatctgg aagctcggga
      481 tgttctccga cgtccgggcc gtcaccgacg gcggcgtcct cgcgttcgag gtcgtcgagc
      541 ggcccgcgat ccgcaagatc ctcgtcgcgg gcaaccacga gctcgcgctc gacaagatcg
      601 acgaggtcat cgagctcaag cgcggcgcca tcgccgacga ggtcgcgatc cgcaagtcgc
      661 gcgacaagat cgccgacctc tacctcgagc acggctttat gttcgcgacg gtcgacagcg
      721 cggtgctccc cgtcgacagc ggcgaggtcg acgtccggtt caccgtcgac gagcacgcga
      781 aggtcatggt ctccgacatc gcgttcaccg gcaatcgcgc ggtcgccagc ggcgagctgc
      841 gcaagctcat ggcgaccagc ccgcccggcg cgctgtcgtt catcaccggc tccggcgtgt
      901 tccgccgcga cgagctcgag cgcgacctcg cgatcctcac cggctactac ctcgaccacg
      961 gcttcgcgac cgtcaagatc gcgccacccg tgctggaccc gtcgcgcgat cgcaagcggc
     1021 tcctggtgac gatcgcgatc gaggagggac cgcggttcac ctacggcgcg atcgacgtca
     1081 agggcgacct gctcggcacc ccggcgcagc acctcgcgct gatccacagc cggcccggcg
     1141 cgacgttcgt gcgctcgatg gtcgaggccg atcgcaaggc gctcgaggtc cactaccagg
     1201 acctcgggtt cgcgcacgcg agcgcggtgc cgcggccccg cgtcgacttc gcgcaccacc
     1261 ggatcgcgat cgacttcgag atcgtgcgcg gcaagcgcgt gttcgtcgag cgcatcgccg
     1321 tccgcggcaa ctcgaagacg cgcgacaagg tgatccgccg cgagctgcag atcgccgagg
     1381 gcgagctgtt cagcgcgacc aacctcgagc gatcgcggca gcgcgtgctg gcgctcggct
     1441 acttcgaagc cgtcacgatc gcgacgcgtc gcgggagctc cgacgacatg atcgaggtcg
     1501 agctcgaggt ccgcgagcgc tcgaccggca cgttccaggt cggcgccggg ttctcgtcga
     1561 cggagtcgtt cctcgtgcag ggccaggtcg cgatggacaa cgtgctcggc cgcggccagt
     1621 cgctgtcggg ccaggtgcag ctgtcgagcg tgcgccggct gttcgcgctg cacttcgtcg
     1681 agccgtacgt cctcgacagc cggtggacgc tcggcaccga gctctacaac cagaccagca
     1741 gcctcggcgc gtacgcgcgc acgtcgacgg gcggctcgct gacgctcggc tatccgctgg
     1801 cggatcacct gcacggcttc ctcacgtacc ggctcgagga cgtgggcatc acgaccgggt
     1861 tcggtggctt tgcaggcctc ggcgcgaccg cggtgcagct gccggcgctc gacaccgcga
     1921 acctgtttcg cggcgggtgg acgtcgtcga tccgcggcgc gctggcctac gacacccgcg
     1981 acaaccggct ggtcccgacg cacggcgcgt acgccaccgc gtacgccgag tatgccggcc
     2041 ggccgaccgg atccgagaac gagttcgtgc gctacggcgg cttcgcgcgg cgctaccggc
     2101 cgctcggacc gttcgtgctg cgactcaacg ccgagttcgg cgtcacccgg tcgctcgacg
     2161 gccgcggcgt gcccctcacc gagcgctacc tgctcggcgg gatctcggat attcgcggct
     2221 accagccgcg atcgctcggc cctcacctgt ggtcgcagca cccgggcgat gtcggccagc
     2281 cgctcgatcc gctggcgatc ggcggcaacc tggagctgat cggcaacgcg gagctcgagt
     2341 tccccctcgt caagagcatc gggttgtccg gggtcgcgtt cttcgatatc ggcaacgcct
     2401 acaacctcga atcgcggttc tgcacccgtc cgggctccga tgcgtgcacc cgtggcttcg
     2461 acgccatcac gagcctccgc aagtcggtcg gcctcggggt gcgctggcgg tcgccgatcg
     2521 gcccgctccg cctggagtgg ggcctcccgc tcgatctcaa gcgcggcgag aagccggacg
     2581 ggctcgagtt ctcgatcggc acctcgttct gacgtcgaga ccgcgggcgg cgctggcgat
     2641 cgtggaccag ggcttgacat gcaaggcgtt gcttgctaat tgtcaggcaa gtaaatactt
     2701 gcctgtagcg acatgccgga gcgccgcgac accgatctgc tgttcaaggc cttggccgat
     2761 cccagccggc gcaagctgct ggacctgctg cacgcgcacg acggccggac cctgaccgag
     2821 ctatgcgagc acctggacat gacgcggcag ggcgtgacgc agcacctcga cctgctggag
     2881 gcggcgaacc tggtcgtgac cgtgcggcgt ggccgcgaga aactgcactt cctcaatccg
     2941 gtgccgctgc aggaactcta cgagcgctgg attgccaagt tcgaaaagcc acgcctgaag
     3001 gcgctcgagg atctcaaacg acgactggag aaagcgaatg gctagatcga cctttgtcta
     3061 tgtgacctac atccgcacga cgccggagaa gctgtggtca gccctgaccc acgatgtgga
     3121 gttcatgaag cagtactggt tcggcaatca ctgcgacagc cagtggacgg cgggctcccc
     3181 ctggaagaac gtgtcccccc agggtgtcgt cctcgacgcg ggcgagatca tcgaggccga
     3241 cccgccgcgg cggctggtga tccgctggca ccatcagcac aagcccgagc tcgaggccga
     3301 gggcgcgtcg cgctgcacga tggagctgga gcccagtgga gcggcggtca agctctcgat
     3361 cacccacacc atcgagcgcg agccctcgaa attgatcgag gccgtgtccg gcggctggcc
     3421 gaaggtcctc tccaacctga agtccctgct ggagaccggc tccatcgctc tgcaggagcc
     3481 ctacccccat tgagagcgcc cggctacagc gggcggggca tcgacgccac gctgcgcgtc
     3541 accgatgcga cgaactgcag cgccgagccg gtcgcgtgca cggcctccac gaccgcgtgc
     3601 gtgccggttc cgctgcgcct cggctggctc gcggtgcccg gacccttcgg gttccccagc
     3661 tcgttcgcga tcacgctgag cgccgacatg acggcactgt acccgagcgg cgccacgccg
     3721 acgatcacgt ggaccgcggg ctgcccgctg tcggtcgcga cctccaacct gagcgcgacc
     3781 atgcccgcgc ccggctgcaa ggtcacggtg accctccaca cgaacaacaa gacgttcgcg
     3841 gtcagcgccg tcccgaactg atcgccgcgt ctcgcggcgt gcgccatcct cgcgatggac
     3901 accggacagc ccgagcgacg gccccacgac tacatcggcg gcggctgtct ggtgcgcgcg
     3961 tcgtccacct cgcagagaac gtccaccgca cacctttcgt gacgtcctcg ccttgcgagt
     4021 cgtcgtcgtg cgagcctgga gatcgctctc gacatcggtt cctgtcctcg agcgttgtag
     4081 atagaatcgc cagcatgagg gagtgcttgg tcgtgctcgt gacgatggtg gcttgtgaat
     4141 atccaccact gccagacatg aataccgatg cgatctcctg cgcggcgggc acggcacaaa
     4201 tgccatcggg cacgccgacc tcgcggcccg tgtgcgcagt gtgcgcggtg gggacgtact
     4261 gcgccggcgg tacggcgccg agtcagccat gcgcggccgg gagctgggac cacgatggca
     4321 atccagcgac ggcgtgcgtt gcgtggacga gctgcttgcc tggacaggcg gtggccacca
     4381 gcggtaccgc cacgaaggac cggacgtgcg cgaagtgcgc gagcgggaca ttcagcacga
     4441 ccgcgaacgc ggcgagctgc acgacttgga caaactgcca gcctggcgcc ttcgtgagcg
     4501 caaatgggac atcggtttcc gatcgccagt gcgcgcaatg cgcggcctgc tccggtagca
     4561 ccgatgtcga ctcgccttcg tgcgcggtcg atccctgctg cccaagcggc tgcccctgcg
     4621 atcccccagg ctcttgcggt cgtccgtgaa gatgcgcagg tcgaccctcc cacccacaac
     4681 gactgtgacg acccgccggt cagcggctca ccgccgcacg ctcatcgctg cacctgggcc
     4741 ttgcgtcgct tgacgcgcgg ccggggcccc ggcggatggt ctggatcggc acgtgcgagc
     4801 agctcgcgct gttcgcggta gaacgccgcg aacgtatcgg cgcggatgtg cgcgcgcatc
     4861 gcggccatca ggcgcacgta gaagacgagg ttgtgctgcg cgagcagttg ccacccgagc
     4921 ggctcctggc acttcatcag gtgatgcagg tacgagcgcg agtgccgcat gcaggcatcg
     4981 caagcgcagg ctcgatcgag cggctgatcg gcaagcttgt acacaccgcg ttcgagatcg
     5041 agccgaccgt gcgacgtgaa cgcgacgcct tgctgcgcca gtgcggtcgg caggatgcaa
     5101 tcgaacatgt cgacaccgcg atgcacgcct tcgagcagat cgagcggcgt gccgacgccc
     5161 atcagatagc gaggacggtc cgccggcaag agctcggtga cgagctcggt gatgtcctcg
     5221 cgctgcgagc gcgtctcgcc gaccgccagg cctccgatcg cgtagccatc gaagccatcg
     5281 gtgttcgtca ggacctcggc gctcgttcgt cgcaggtctg gaaagcacgc gccctgcacg
     5341 atcgcgaaca gggccgcggg tggggcatcg aggtcggggg cgggaggcgc agcgcgatgt
     5401 gcggccagcg agcgattcgc ccagcgatgg gtccggtgca tcgcagcttc ggtctccgcg
     5461 cgcgagctcg tcgatgcgat gcagtgatcg agggccatca tgatatcgga gccgatcgca
     5521 cgctgcgccg cgatcgattg ctcgggtgac agcagcaggc tgcggccgtc gaggccgtgt
     5581 aactcggcgc ccgcttcgct gatcgtgcac ggtagcgaga acgcctgaaa cccacccgag
     5641 tcggtcagga tcgagtgcgt ccagcccatc cagcgatgca agccgccgaa gcgctcgaag
     5701 acgtcgaggc ccgggcgaac cagcaggtgg tacgtgttcg cgagcaggat cggcgcgccg
     5761 agctgctcga gctgcgcgag cgtctgggtg cgaaccgtgg cgcgcgtccc gaccggcatg
     5821 aaggccggcg tctcgacgcg atcgtgcgag gtgaccagcg tcgcgacccg cgcccgcgag
     5881 ccggtcgccg tcgcgtggat ctccagcctc atggtcgcga cgtgttagca tcgtgcgcgt
     5941 ggctgcgata gctcgactgc gaggtctcgc gccaccgggg acacgcacgt cggcgtcgtc
     6001 ctgtggtcgt cgatggcatg acgttgatca cggcgcagcc cttcaccgac aacctggccg
     6061 cagccatggg acttccgacg gtgttcctcg gctggggcgc cccggcacgc cagtgcggtc
     6121 tgccgaggtc agcgacgcac tccgactccg cgagaggcgc cgtgcgccgg ccgatccgtc
     6181 gagggtgacc ggaccaaacc ggcggtggtg gcgaggcgat gcgtggccag ttctcgcagg
     6241 ccacggcggt ctcgtcggct cgtcgccgcg ggcgcgcccg ttgcaaaagc gatggggcat
     6301 ggccaccacc accaccacca ccaccggacc tggtcccgaa cccgcacctc cgttgcgcag
     6361 tgagctcgtc gagggggtgc gaacgacgtc gaacgcgcga tggggcgcca ttatcggtgg
     6421 cgcgttcatg gcggtggcga gctggatcgt cctccacctg ctcggcctcg gtgtcgggct
     6481 gaccgtgatc gacccgaacc agacaagctc gttgcgcgcg atcgggctcg ggaccgggat
     6541 ctggagcctg atcgcgccga tcatcgcggt gttcatcggg gggctggtgg tcagccgtct
     6601 cgcgcccacg ccgagtcggc tcacgcgcgt gatccacggt gggctggtct gggcggtcac
     6661 cacgctcgcc accatcacgc tgctcgtggc gctggcgtcg agcctggtga ggggtgccgt
     6721 gtcgaccggg ggacaggtcg tgagcaccgc ggcaggtgcg gtggggggcg tcgccggtgc
     6781 ggtcgaccgc gacgcgctgt cctcgctcgg catcaagagc gacgatctgc tcgcgccgat
     6841 caaccagcga ctccgtgccg agggcaagtc cgaggtgacc gcgcagcagc tcgaggccgc
     6901 gctcaaggac acgctgcgca ccgcggttcg caccggaaac ctcgatcggc agaccctggt
     6961 caacgcgctc gccaccaaca caccattgac gccgcgcgat gcggaccaga tcgcgaacag
     7021 cgtcgaggct cgctggcaac aggtccagca gcgaggctcc gagctcgcga accgcgcctc
     7081 gaccgccgcg ctcgaggccg cccaggcgac cggcaaggcg ctcctgggcc tcgcgatcgc
     7141 gctgatcctg gggctggtcg cggccgccgg cggttcgctc ctgaccggga acatcgaccg
     7201 tcgtcgccct cgcccggcct cggagtgatc tgcgccctga aggggtactg cgtacgggcg
     7261 cgttccgcgg gccgcgcgcc ctctccgagg tgtcgaggtt gcccggcaac gggatggaac
     7321 gccggcgtgc tggtcgacag gcgccactga ctgtatgatg cagcgatgcg gatgtggctc
     7381 gtcgcgggtg tgaccgtcgt cggctgcggt ggctgcagct cgagcaagag cctcgactgt
     7441 gcctatctcg ccggcaacaa ttgctggaag accaccgttg ccgaggctac gacctgcctg
     7501 cccgcggcgg gcacgacggg gaagctgagc gccgacaact cgagctgcgc gtacgcgagc
     7561 gggcagacga tcacgttcac accggcgctc gggttaccgc tggccagcgg gttcaaggac
     7621 tggaacttca cggtcacgac cggcgggcaa ccctgcttgc actacgagga gagcgccggt
     7681 ggcttcgagc tgaccgtccg cgcggacgcc gtgagcgaga tgatcagcgg cgcggccggc
     7741 atcgacctga cgtgtcccga cggcacgacg tactccaact cgaacgcgct cacgctgctg
     7801 gactgcccgg gcagcgcgtt cggagacctg cccgggaaca cgacatcgtc ggactccacg
     7861 agcgtcacgt tcggcctcat caacaccggc acgccgagct cggtcacggt gttcgactgc
     7921 agccgctgac cggtttgcca gcacacgctt gcggacggca ccacgcgtcc ggcgagtaca
     7981 cggtcttcgc ggatcacggc gacgcggtca gcagcggcgc gagctcggcg gcaaacgcga
     8041 cgggatcgac ggcatagccg acgtggccgc cgacgaactc gaccagccgg acgccgagcg
     8101 acgccgcgag cacccgggcg ggccgtgcca gcggcgcatc gggcggctcg cggcctaccg
     8161 cgaggacgag ctggccggcc gcggcgcgta gcgcggcgag atccggggcg aaccgcggat
     8221 acgtccgcag ctcgcgcgcg agccagaacg cgcggttctc cgggagccgc gcgagcatcg
     8281 ccgcgatctc cggcgacagc tgcatgctcg gcggtggccg cagcaccggc atgccgaccg
     8341 cggggccgaa ctcggccatc gcggcctcga cgcccgacgc gaggtaggtg gcgtggacgt
     8401 gctcgaacat cgcgagccag tgcgcggcgt cgggcagcag cgtcaccagc ggcggctcat
     8461 gcgcgacgag cttgcgaatg cagccgggcg agcgcgcgag caagtcgagc ccgacgatcg
     8521 cccccgagct cgaaccgagc acgtacgccg ggccgccatg gcgctcgatg acgcgcatgg
     8581 catcgtcggc gtcgagcgcg agccgcgcgg cgtcatcgac cggcgcgtcg agcgggctac
     8641 gcgcgtagcc gcgccggtcg taggcgacca cggtgaactg ccgcgcgagt agcgcggcga
     8701 gccggccgaa cgactcggcg tcgccgttgc cgccgacgat gcacaccagc agcggcccgg
     8761 agccgcgggt ctccgtgtac agccgcgcgc ccggcacgtt tagcatctcg atcttcttct
     8821 ccataggctc tccggtcatc tctgcggcgg tcatcgctcg ccgatcacca gcgcagcatg
     8881 cgccgccgac gtttcacaac cggccgcggt gatgtgaaga aaccttgcgt cgcctgcgag
     8941 ctcttcgcgt cgcgccgttc cgacccccgg ttggggtgtc ggctcgccga ggggcgtccg
     9001 cgaggttccg agcgtgcttg gggctgccgg aggagctggc ggcgtgactc actgctgtgg
     9061 caggggaccc gagcgcggcg agcaggcgcg cgaactcgtg cggcggcacg ctgccaccgg
     9121 tcgcgcgtgg atggccgttc gcgaactcgc cctcgacacg accgaggccg aggccccgca
     9181 acagcgcgag cagatcgacc tgcgacgcgc tgcgcaccgc gaagttcacg cgcccggggc
     9241 ggcgcgcacg gcggcaagcg cgcgagcctc gaacgcctgg ccgcggggct ggcgcgcctg
     9301 tcgccgcggg cgcgtgcgcg ggtcgtgctg gagaacgacg atcgggtgta caccgtcgag
     9361 gatctgctgc cgatctgcga gcgactcgag gtgccgctgg tctacgacgt ccaccatcat
     9421 cggtgcaatc ccgaacggcg gtcgatcgag cgcgcgaccg agctggccgc cgggaccttc
     9481 ggcggccgcg agccatgggc tcacctgtcg agcccggcgc tgggctggaa cggcgccgat
     9541 ccgcgacccc cacgccgact acatccgccc gcgcgacgtg tccgcgtgct ggctcggacg
     9601 caccctgacg atcgacgtcg aggcgaagga gcgcgcggtg ctgcgcctgc ggcgatggct
     9661 gaaccgcgcg acgcgcgagg cgggggtcgc cgcggttcgc ccggagctgc caccgggggc
     9721 cgcaaagacg gccactcgca tcccaggtca ctccagtgcc aaaggagcgg atgcaacaaa
     9781 tgatctcggc gctgtccggg ggcttcctga ccattcgtgc aggctcgctc gacgcgaagc
     9841 aatccagcct ggggttcagg tcaccgccgc cgaagcaatt tgcaagcgtg gatccctggg
     9901 ccggggtata tgtcgggccc ttgccgtttc ctcctgccca tcctcgcctc ctctccgcgc
     9961 tcgcggaccg ccagtacctc gagccgacgc ccgtccaggc ggcggtgctg gccgacgagg
    10021 cgcgcgaccg cgacctgctg gtctcggcgc agaccgggtc cggcaagacg gtggcgttcg
    10081 gcctcgcgct cgcccacacg ctgctcgggg acgcgccgcg gttctcgccg ggcggagcgc
    10141 cgcgcgcgct gatcgtcacg ccgacccgcg agctggcgct ccaggtccag cgcgagctcg
    10201 tctggctgta cgccgagacc ggcgcgcgga tcgcgtcctg cgtcggcggc atggatccgc
    10261 ggcgcgagca gcacgtgctg cggcagggcg tacagatcgt ggtcggcacg ccgggtcggc
    10321 tgtgcgatca cctcgaccgc ggcgccctcg tgctctcggg actgcaggcg atcgtgctcg
    10381 acgaggccga cgagatgctc gacatggggt ttcgcgagga tctcgagcgg ttgctcgagg
    10441 ccgcacccac cgagcgccgc acgctgctgt tctcggcgac gatcccgagg gagatcgcag
    10501 cgctcgcgaa gcgctatcag aagcacgcga tgcggatcgc gacctcgacg gaggccgggc
    10561 gccatcacga catcgagtat cacgcgatcc cgatcgtgcc gcgcgagcgc gatctggcgg
    10621 tggtcaacgt gctacgatat catgaggccg ccggtgcgct tgtgttctgc gcgacgcgcg
    10681 acggcgtggc gcacctcgcg gccaacctcg ccgagcgcgg gttcgccgtc gtggcgctgt
    10741 cgggcgagtt cagccagcgc gaacgcaaca cggcgctcca ggcgctgcgc gatggccgcg
    10801 cgcgagtgtg cgtcgcgacc gacgtggcgg cgcgcggtct cgatctgccg gacctcggcc
    10861 tcgtgatcca cgccgacctg ccgcagaacc agcaggtgct cgtgcatcgt agcggacgca
    10921 ccgggcgcgc agggcggcac ggcgtcgcga tcctcctcgt cccggatccg gcgcgccggt
    10981 acgtcgagcg catgctgcgc gccgcgcacg tcgatgcgac gtggtcgcgt gcgccgacgg
    11041 ccgacgagat cctcgcccgc gaccaggagc tcgtgatcgg cgagatcgag agcctgaccg
    11101 aagagctctc cgac
//
LOCUS       DASVOR010000155         2712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_322939,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000155 DASVOR010000000
VERSION     DASVOR010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2712
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322939"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF516_03530"
     CDS             <1..204
                     /locus_tag="VF516_03530"
                     /inference="COORDINATES: protein motif:HMM:NF019013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HEX8106770.1"
                     /translation="KTVARVRVTGTHKGEFMGVAPTAARVDMQLIDIMRFDGAGLVCE
                     HWGVADMLSLLQQLGVVPAGPPA"
     gene            407..679
                     /locus_tag="VF516_03535"
     CDS             407..679
                     /locus_tag="VF516_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106771.1"
                     /translation="MATYIGIHEVDDIGHWLRSPKRQELFGPLGVTGHLFTDTAKSNR
                     VALILEIPDMEAFQKLLQSQAGADAMKFDGVRPETIAIFEKATDHV"
     gene            985..1278
                     /locus_tag="VF516_03540"
     CDS             985..1278
                     /locus_tag="VF516_03540"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HEX8106772.1"
                     /translation="MPVKVIIELQAKPGRRAELTSLLETMIATQGSGQHGFLGSTRYE
                     TLDDADTLVEIADWDSAEARTAHMQQAAATGAYTPLRELLAAPFRATVIRQPS"
     gene            2290..2358
                     /locus_tag="VF516_03545"
                     /pseudo
     CDS             2290..2358
                     /locus_tag="VF516_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689697.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2500..>2712
                     /locus_tag="VF516_03550"
     CDS             2500..>2712
                     /locus_tag="VF516_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106773.1"
                     /translation="MPDNTELIMRFHPVGGEDVSVLSKDFGGEQEALERIAHSMDERR
                     SLVLRRARYDREEAENGMVVNLANVVS"
BASE COUNT          463 a          918 c          898 g          433 t
ORIGIN      
        1 aagaccgtgg cgcgggtgag ggtcacggga acgcacaagg gcgagttcat gggggtggcg
       61 ccgaccgccg cgcgcgtgga tatgcagctc atcgacatca tgcggttcga cggcgccggc
      121 ctggtgtgcg agcactgggg cgtcgcggac atgctctcgc tcctgcagca actcggcgtg
      181 gtgcccgccg gcccgccggc atagcccccg ccgaccgttc ccgccgaacc ctgctggccc
      241 ccggcgtccg ttctggcctg cgcgggtcga ggcgcagccg ggcgtcagtc gatcgagtga
      301 ggtcttcgcc gctcaccgcc tgcactctca tttcctatcc tgccgcaaat gcggcgacaa
      361 gcagattcgc cgctagctca gtcctgcgac ctgaggagtt cgtcacatgg caacgtatat
      421 cggcatccac gaagtcgacg acatcggcca ctggcttcgc tcgccaaagc ggcaggagct
      481 cttcgggccc ctgggagtca ccggtcacct cttcaccgat accgcgaagt cgaatcgggt
      541 ggcgctgatc ctcgaaatac ctgacatgga agctttccag aaactcctgc agtcgcaagc
      601 cggggccgac gcgatgaagt tcgacggtgt ccgccccgag acaatcgcga tcttcgaaaa
      661 ggcaaccgat cacgtctgac ggcgtgcgct cgcgtgcgtc cgcgccctgc ggggtgatgc
      721 gagtcgggga cgcccctggc ggcgaagact ccatcatcgc ctcggcgccg gtgcgcaccc
      781 ccgtggtccg acaccccgcc gcacaccctc cgtccaccag tcagcgagcg catcagtgaa
      841 cgagcacccg aacgccgtga tcgtccgatc ggcctacgag gccatggaaa aaggagacgt
      901 agccgccttc gccgagctgc tcgacccaag ccgaggcaca cggggtcatc cagcccatga
      961 ccgtctactt cgaggcgtag gcagatgccg gtcaaagtga tcatcgaact gcaggccaag
     1021 cccggtcgac gggccgagtt gaccagcctg ctcgagacca tgatcgcaac acaaggatcc
     1081 ggccagcacg gattcctcgg cagcacccgg tacgagacgc tcgacgacgc cgacacgctg
     1141 gtcgagatcg ccgactggga ctccgccgag gcgcgcaccg cgcacatgca gcaagccgcg
     1201 gccaccggcg cctacacgcc cctgcgcgag ctactggccg cgcccttcag ggccaccgtc
     1261 ataagacaac cgtcctgacc gaagccgcca ctgccgccga cagacgctga gcagaacatt
     1321 tcggcactgc ggtgcaggtg ttctggtgtc gccgagaatc tccgtccccg ccggggcggt
     1381 atcgcctggt cgcgaccaag cccgacacct cgcaccggtg gcttgccggg tcagggatgc
     1441 cccagccgca gccgcgctgg ggtgcggcct cattggagtt ggtatcgccg cgatcttaac
     1501 cactgatcgg gatacggttc gccggtgctc gtcatcgcct ccctcggaga cccactcgag
     1561 agcctccggt acgcgatctt gagctagtcc tgcgactgaa ggcggatctt gacgtcgagc
     1621 agaaaaggta atgatcagag gtggtgcccc cggcaggatt cgaacctgcg acacccgctt
     1681 taggagagac ctcgtcttcg ctctcatgtc cgctgaccag cggtttcatt ggtgctccag
     1741 tgggactcag aggctccacg gggctctctt ggcacgagtt cgccccacga atggctccac
     1801 ggtcagggct ccctgcgccg cggctccctg gcagggggag ctcagctatg ggcgccgcaa
     1861 gaccagaggc ggcgtcaccg gccggtggca tggactcatg cgcttcgccg gctcgctgga
     1921 ctccagcgcc gcggcagaga gcctccggcg gtgagccagc tgccgagagc tcccacgagt
     1981 tcggtcattc ggaggatctc atgccggccc atccgctgtc cgatgccggc gtccgcgaca
     2041 ccagccatga ggtcgacgag accgagcagc tcgtcaagcg ccgcgtcggg gtccatcagc
     2101 gaccaccgga acgcagcacg aggcgagagc gggcagcccg gtgggccgcc cgccggtggg
     2161 ggttaccacc tgccctcgtc ggcggtgagt tcgtcggcga ggcggcgctg gtgctcgccg
     2221 cggtcatcgt cgtcgcgcca gtcggtggtt gtggtggtca tggaggtctc cgcagcattc
     2281 ataccgggta ccaacgcgat cgcgtccctg aacgcccgct accggcgcgc cgtccgcgcc
     2341 cgaggccact tcccgaccgg gctgacggca ggagtcgtac cgctgccgtc catgccccga
     2401 gctggaggag actacagagc gtccgatcgc ttgcccaacg gtctcggagg gagccggcgg
     2461 tcgggcggag gctggattct cggtcgggaa gggcttgccg tgcctgacaa taccgaattg
     2521 atcatgcggt ttcatccggt cggcggagaa gacgtctccg tgctgagcaa ggatttcggt
     2581 ggggaacagg aggcgctgga gaggatcgcg cattcgatgg acgagcggcg cagcctggtg
     2641 ttgcgccgcg cgaggtacga ccgcgaggag gccgagaacg gcatggtggt caacctggcc
     2701 aatgtggtgt cc
//
LOCUS       DASVOR010000156         2671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_59880,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000156 DASVOR010000000
VERSION     DASVOR010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59880"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF516_03555"
     CDS             <1..244
                     /locus_tag="VF516_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106774.1"
                     /translation="STLSMAQLIVRNIDQILVDLLKRRAAEHGRSAEAEHREILRAAL
                     QPRAEASLKDHLLAMPDVGNDADFVVKRSRARRVML"
     gene            241..651
                     /locus_tag="VF516_03560"
     CDS             241..651
                     /locus_tag="VF516_03560"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8106775.1"
                     /translation="MKFLVDTNVLSEVRKGPRANARVLGWFGSVDDAALYLSVLVIGE
                     LRQGVERLRRRDPPAAAKLDRWLDELIACHADRTLPVDAAVADRWGRLNVPDPIPAVD
                     GLLAATALVHSLTLVTRNTRDVRRTGVRHLDPFA"
     gene            complement(745..1755)
                     /locus_tag="VF516_03565"
     CDS             complement(745..1755)
                     /locus_tag="VF516_03565"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HEX8106776.1"
                     /translation="MRGCSCTAIRSIAGLDARLGTAHVSEPGDAAPAASRLALARNGM
                     IALNALFLGYNALDAVYLWGGHAPSGITHTEYAHAGTAWLTIAFVLSTIVLGALFRGP
                     IEHHARGRFARRLAYAWAAQNFLLAAGTFRRITMYIAYSGLTQLRIVGIFGTTLATLG
                     LAIVVYKLARRRTMLWVLHRQLDALALAVAVFIVAPTDDLAMRYNAARIAADQYRPLL
                     HLYEQPIRPEAVPALLPLLDHPDPVVREGVAVIAAVQRDRLRADDARAGWLDFEISRH
                     RALGALDDAAAQLAAVLPRDRQAATARLRGVAYGINDEVERAGDEGPFNWRTRRYAPR
                     DD"
     gene            1982..2635
                     /locus_tag="VF516_03570"
     CDS             1982..2635
                     /locus_tag="VF516_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106777.1"
                     /translation="MLGLALERQGARLARLQVLLEIDVAREPPAADPEQRHRHQRPGK
                     RGEQRAAHAEQPRQHAPDPEHQADARRRVRAGADQPRRARPVERKQVPLQGRRDEQER
                     GAHQHPDRQRHQVEAEAALQGEHRHHDDRDRKQPPGCRQPRHGLREHPRERQHVAGPG
                     GHGDEDTIAHRPASRDHDTARDEILELAGVRYDPELVAVFHGLDRAAWRAVAEQHPG"
BASE COUNT          412 a          952 c          968 g          339 t
ORIGIN      
        1 atcaacgcta tcaatggcac agctgatcgt tcgcaacatc gaccagatcc tcgtcgacct
       61 gctcaagcgc cgggcggccg agcacgggcg ctcggcggag gccgagcacc gcgagatcct
      121 gcgcgccgcg ctccagccac gagccgaggc gagcctcaag gatcacctgc tcgccatgcc
      181 cgacgtcggg aacgacgccg acttcgtggt caaacggtct cgcgcccgcc gggtgatgct
      241 gtgaagttcc tcgtcgacac caacgtgctc tcggaggtcc ggaagggccc gcgcgcgaat
      301 gcccgggtcc tgggctggtt cggatcggtc gacgatgccg cgctgtatct ctcggtgctc
      361 gtgatcggag agcttcgcca gggggtcgag cgtctacggc gccgcgatcc gcccgcggcc
      421 gcgaagctcg atcgctggct cgacgagctc atcgcgtgcc acgccgatcg gacgctcccg
      481 gtcgatgccg ccgtggcaga tcggtggggc cgcctcaatg tccccgatcc gatccctgcg
      541 gtggatgggc tgctcgcagc gaccgcgctg gttcattccc tgaccctggt cacacgcaac
      601 acgcgcgatg tccggcgcac cggcgtgcgg cacctcgatc cgtttgcctg acggtcgacc
      661 cgcaacgccg agaacccgag cgcggtcctg cgcaccacct gccggcgcga ccgcgggaac
      721 ggctgcgcgc ggccgtgcac atgctcagtc gtcgcgcggg gcgtagcgtc gcgtgcgcca
      781 gttgaacggg ccctcgtcgc ccgcacgctc gacctcgtcg ttgatgccgt aggcgacgcc
      841 gcggagccgg gcggtcgcag cctggcgatc gcggggcagg acggcggcga gctgtgcggc
      901 ggcatcgtcg agcgcgccca gcgcgcggtg gcgtgagatc tcgaagtcga gccagccggc
      961 gcgcgcatcg tcggcgcgca gccgatcgcg ctggacagcg gcgatcaccg cgacgccctc
     1021 gcgcacgacc ggatcgggat ggtcgagcag cggcagcagc gccggcaccg cctccgggcg
     1081 gatcggctgc tcgtagaggt ggagcagcgg gcggtactgg tcggccgcga tgcgcgccgc
     1141 gttgtagcgc atcgcgagat cgtcggtggg cgcgacgatg aacaccgcga ccgcgagcgc
     1201 gagcgcgtcg agctggcggt gcagcaccca cagcatggtg cgccggcgcg cgagcttgta
     1261 cacgacgatc gccaggccca gcgtggcgag cgtggtcccg aagatcccga cgatgcgcag
     1321 ctgggtcagc ccgctgtagg cgatgtacat cgtgatccgg cggaacgtgc ccgcggcgag
     1381 caggaagttc tgcgcggccc aggcataggc gagccggcgg gcgaaccggc cgcgcgcatg
     1441 gtgctcgatc ggtccgcgga acagcgcgcc caggacgatc gtgctgagca cgaacgcgat
     1501 cgtgagccag gccgtgccgg cgtgcgcgta ctcggtgtgg gtgatgccgc tcggcgcatg
     1561 gccgccccac aggtacacgg cgtcgagcgc gttgtagccg aggaacagcg cgttgagcgc
     1621 gatcatgccg ttgcgcgcga gcgccaggcg cgacgccgcc ggggccgcat cgccgggctc
     1681 ggagacgtgc gcggtgccca gacgcgcatc gaggcccgcg atcgagcgga tcgccgtaca
     1741 cgaacagccc cggatcgcgg atcaggtgca tccggaacag cgcgtcgacc agctcgatct
     1801 ccctggcgac cggccagtgg gtggtgcgga tcgcggtcat gatcgcatgc agcatcgaag
     1861 acagcgcgag gatcgcgcgc tcggcgacca cgccgtcctc gcggcaccac tcggcgatcg
     1921 cgttcagcgt gttgaacagg aagtgcgggt cgaagtggcc tcgcagcgcg agcagctggg
     1981 cgtgctcggc ctcgcgctcg agcgccaggg cgcgcgcctg gctcgcctgc aggtgctgct
     2041 cgagatcgat gtcgcgcgcg agcccccagc cgccgaccca gaacagcgcc accgacacca
     2101 gcgtcccggg aaacgtggtg agcagcgtgc tgcccatgcc gagcagcccc ggcaacacgc
     2161 gcccgatccc gaacatcagg ccgatgcccg ccgccgcgta cgcgccggcg ccgatcagcc
     2221 gcggcgcgcg cgcccggtgg agcggaaaca agtaccgcta cagggtcggc gcgacgagca
     2281 ggaacgcggc gcacatcagc atcccgatcg gcagcgccat caggtcgaag ccgaagctgc
     2341 gctgcaaggc gagcaccggc accacgacga ccgcgatcgg aaacagccgc cggggtgccg
     2401 ccagccccgc catggtctcc gcgaacaccc gcgagagcgg cagcatgtcg cgggccccgg
     2461 cggtcacggg gacgaagata ccatcgcaca ccgtccggcc tcgcgcgatc acgacaccgc
     2521 gcgtgacgag atcctggagc tcgccggcgt gcgctacgat cccgagctgg tcgcggtgtt
     2581 ccacggcctc gatcgcgcgg cgtggcgcgc ggtcgccgag cagcaccccg gctgagccgc
     2641 cgggccgcta tcccggccgg cgccggagcg t
//
LOCUS       DASVOR010000157         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_118414,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000157 DASVOR010000000
VERSION     DASVOR010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118414"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..772
                     /locus_tag="VF516_03575"
     CDS             <1..772
                     /locus_tag="VF516_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106778.1"
                     /translation="LPSIHGVVAAPAQHLVLATATAEKTLALIDDTTLQVKSRVSAGE
                     YPNGLAFDPASEKVFVSNNQGRGVAVVDVKTARALPGIDIGGGAGNTQWDAESGHVLA
                     AVHGSPSLVDIDPAKSQVAGRVTLHDVTTCHGLLVASPLRLAFAACRGAAPLLAVVDL
                     RTRRQTMILPLPGGIDVLAFDPGLQRLYAASETGMVAVFAVAADRSVTEMGRGFLGPN
                     AHSVAVDPTTHRVYFPLENVGGRPVLRVMDARAATGSP"
     gene            complement(979..2430)
                     /locus_tag="VF516_03580"
     CDS             complement(979..2430)
                     /locus_tag="VF516_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106779.1"
                     /translation="MSAQDLATHLSLITGAMLLITPALIRRGEIGGIGTTICMLGAAG
                     TAVLAVLAYLFARKRGATAAPRMTIREWIIKRGVIYALTQAPVVAIFAGFFFRIVPFG
                     VAAIGEIIAFFVLWIWVAHRKAVSRDPDEPVHHVTRYALYTLVPCVAFSLARIPTHLG
                     FGFAFWHPWYEFGSELTGLPLHHYPSLFAGAMLYSLDGVVLTLGYYVLFQKRSLVNAI
                     LYIGLYISSIYCFVFPAYARIGMQSPPMWHAVVFWAHIVMAVTAWYMPRFFERTWPRL
                     RSLGRSAAVIAIASALVGPYAYAAVQSRALQWPKQHAIDAALFARPDLLAASDHAAVT
                     VTGTEARYDLTMQLGSRSYKSWVNRTRMLDVRDLEITGRLLRDGAPIAWCTAHVSELP
                     RINEAASEPDFLELVRTHDVAQIPVRCMGSSAVLRSDDLGPVTLDWSASATLIGDRES
                     TQRTFTGSRPLRVIGREHLAAVDGTRSSHSALY"
BASE COUNT          526 a         1042 c         1098 g          464 t
ORIGIN      
        1 cctcccgagc atccacggcg tcgtcgccgc cccggcccag cacctcgtgc tcgccacggc
       61 gacggcggag aagaccctcg cgctcatcga cgacacgacc ctccaggtga agtcgcgggt
      121 gtcggcgggc gagtatccga acgggctcgc cttcgatccc gcgtcggaga aggtcttcgt
      181 ctctaacaac cagggccggg gcgtggcggt cgtcgacgtg aagaccgccc gggcgctgcc
      241 gggcatcgat atcggaggag gcgccggcaa cacgcagtgg gacgccgagt cgggccatgt
      301 ccttgcggcg gtccacggct caccgtcgct cgttgacatc gacccggcca agtcccaggt
      361 cgccggacgc gtcacgctcc acgacgtgac cacgtgtcat gggctgctcg tggcatcgcc
      421 gctgcggctc gcgttcgccg cgtgccgcgg agccgcgccg ctcctggcgg tcgtcgactt
      481 gcgcacgcgg cgccagacga tgatcctgcc gctgccgggc ggcatcgacg tgctcgcctt
      541 cgatccgggt cttcagcggc tctacgcggc ctccgagacc ggcatggtcg ccgtgttcgc
      601 cgtcgccgct gatcgctccg tcaccgagat gggccgcggc ttcctgggcc cgaacgcgca
      661 cagcgtggcg gtcgatccga ccacccaccg ggtctatttc ccgctggaga acgtgggcgg
      721 gcgccccgtc ctgcgcgtca tggacgcgcg cgccgcgacg ggctcccctt agcagaccgt
      781 ggtcgacgcg ttcgaggtcg cctgcctgtc ggcgcggatg gcgcgcaacg acggcaacca
      841 tcacgcgctc ggcgcaggag gccggcttga tccgtcacca cctgcgcgag ctgctcgagc
      901 gccacgggct catgggcggc gacgccgagt agcgagctgc ccacggtccg cgatgcccgg
      961 gtaccgtccg aggacggcct agtacagggc ggagtggctg gaccgagtac cgtcgaccgc
     1021 ggcgaggtgc tcgcggccga tgacgcgcag cgggcgcgag ccggtgaacg tgcgctgcgt
     1081 cgactcgcga tcgccgatca gggtcgcgct cgcggaccag tcgagcgtca cgggcccgag
     1141 atcgtcggag cgcaggacgg cgctcgaccc catgcagcgc accgggatct gggcgacgtc
     1201 gtgggtgcgc accagctcga ggaagtcggg ctcggacgcc gcctcgttga tccgcggcag
     1261 ctccgagacg tgggcggtgc accacgcgat cggcgcgccg tcgcggagca gccgcccggt
     1321 gatctcgagg tcccggacgt cgagcatgcg cgtgcggttg acccagctct tgtacgagcg
     1381 cgagccgagc tgcatcgtga gatcgtagcg tgcctcggtg ccggtcacgg tgaccgcggc
     1441 gtggtcggac gcggcgagca ggtcggggcg cgcgaacagg gcggcgtcga tcgcatgctg
     1501 cttcggccac tgcagcgcgc gggactgaac cgccgcgtac gcatagggcc cgaccagcgc
     1561 cgacgcgatc gcgatcacgg cggccgaccg cccgagcgag cgcaggcgcg gccaggtgcg
     1621 ctcgaagaac cgcggcatgt accacgcggt caccgccatc acgatgtgcg cccagaacac
     1681 gaccgcgtgc cacatcggcg gcgactgcat gccgatgcgg gcgtacgccg ggaacacgaa
     1741 gcagtagatg ctcgagatgt agagcccgat gtacaggatc gcgttgacca ggctgcgctt
     1801 ctggaacagg acgtaatagc cgagcgtcag cacgacaccg tcgagcgagt acagcatcgc
     1861 gcccgcgaac agcgacggat aatggtgcag aggcagcccg gtcagctcgc taccgaactc
     1921 gtaccagggg tgccagaacg cgaacccgaa cccgaggtgg gtcgggatgc gcgccaggct
     1981 gaacgcgacg cagggcacga gcgtgtagag cgcatagcgc gtgacgtggt ggaccggctc
     2041 gtcgggatcc cgcgagacgg ccttgcggtg tgccacccag atccacagga cgaagaacgc
     2101 gatgatctcg ccgatcgcag cgaccccgaa cggcacgatg cggaagaaga agcccgcgaa
     2161 gatcgcgacc accggcgcct gcgtgagcgc gtagatcacg ccacgcttga tgatccactc
     2221 gcggatggtc atccgcggcg ccgcggtcgc cccgcgcttg cgtgcgaaca ggtaggcaag
     2281 caccgcgagc acggcggtcc cggcggcgcc gagcatgcag atcgtcgtcc cgatgccccc
     2341 gatctcaccc cggcggatca gcgcgggcgt gatcagcagc atcgcgccgg tgatgaggct
     2401 caggtgggtg gccagatcct gggccgacat gccatacctc gacggtgagg ccgagcgtga
     2461 gagggcgggt gtgagtgcag gtgcggacgc cgattccaca ctgctggtag aattgtgctc
     2521 acggacacca agaatcgtgc cgatgatctg cggggtccac accgtgcccg atgtcccacc
     2581 agaatcatga gtgtcgctca tgggtggcgg tgtcgtgcca caggcaacga ccatctgacg
     2641 cgatgcacta gcaaagcgag tcgaggacag atcgcgctgc ggagaatccc ctgacggctg
     2701 gatcgcggtc ggtgaggagt cggtgtttgt ggcggttgga cagctactca tctcaaaaat
     2761 ccgttgttca gattacgtgc atttcgggac agtcgcgtcc aatgcacggg taatgccgga
     2821 gtcttggtcg ctggctcacc aggcggtggc cgtgggtaga tcgctattct tttcagtgac
     2881 ttctatgatc tcagaggtcc gcggctcgag ggtggacaag gcgagcacga cccgcacctg
     2941 gacgtaatcg cgcaggtccg cgacggcatg ttccggcgct cgatgcgacg cccacacgag
     3001 ctgcacggtc gctgaatgac gtgagtcaac tatcgttaaa ctcacacgca atgcccgcgc
     3061 ccgattgacc tatgcgggca aaggcccggc gatcagtggc tcggtccgcg cgcttaccac
     3121 gctcaccgcg
//
LOCUS       DASVOR010000158         6089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439748,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000158 DASVOR010000000
VERSION     DASVOR010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439748"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF516_03585"
     CDS             <1..204
                     /locus_tag="VF516_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106780.1"
                     /translation="GNVRHISLMDPAKPVVKMQLPGPERDGPAITSRPDLLGQWVRNS
                     YFDYAAGDQISAAAVTTQTFQAQ"
     gene            222..3326
                     /locus_tag="VF516_03590"
     CDS             222..3326
                     /locus_tag="VF516_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106781.1"
                     /translation="MESPMLRYLSAAALALAICACEPSLPQTTPTPTVTAIFDPTAAQ
                     IPLPNDLAFAAPINSVCPPPANALTAPTDPPQCAQAELLKALNPSGVPLAGAFPSDQE
                     VPLTIDFTESTYVNSRVKQVAPDLDLSTITPATLFVIGPAGPIALEPPTPDDYVVPPP
                     VNMVPADHGTLSVHRQGHQPWPPGAYVLVLRGGDQGVKTKATADTPAIPVTPSPVFDL
                     IEQGKDMTAPENLGLLKAQSGGSTADAIAQGQQLNLLIAIYKATAFPAADTVFPHEQL
                     AIASTFHIGPTVTNVEVDPARGLAPLPFDLLRDAGTGKLTPLAACTLAGSSLRKDGTC
                     PSPAAAGFQTLDGFSTTGAILAPTSDLIQASTVTKDTLMLFDLSDPKNPTLVPAGSLI
                     VEPCEFTSSCTGPALAPVIAFQPAGATAVDPTGSPPSVFRTKPLKDSTDYAVVMTTDI
                     KDKAGNAIGPGTVAKILQFKNPLTVGTHSALLGIDDATAASLDKMRKQLAPVFTALGA
                     NATKVAMAYTFHTQTVLGHAVDLAALPYSTPTVTAGTGPVTPPPVNPPDPAADDSPRN
                     AFTKFGVIPRTAPADTTPGHVPLADIDEVLETTITTFNALDPATGAFLPDPSKAAPET
                     IKVLIATPKATAAPDCPAALSAFNKCAPLVVFRHGLGRGRADMLLIANAFAAAGMVTV
                     AIDAAKHGDRTLCSSAATPPLSGCAGTCTPLSNAPDPIDPPSQGPPGTCDPTKLIKAP
                     VDKNATGATDGIAVASGNYLISANFFRTRDTLRQDLIDESQLIRVVALDPSKPPMTTL
                     LFERIKSRAMAKFGVTMIVDPTAVYFSGQSLGAIQGAMDVATNPRISRAAFNVGGGTL
                     VDVFATAPAFHAGFTQLISALGITPGTSQFLQFLTVAKTVLDPADPINFVGHLTDKDH
                     MLPNLLPPLGNPNGLVPQQPKAILSQIANCDDTVPNPFNFLYASNAGLSPLPPSGQPG
                     TVQQFVGAGFDPAKPTLCSPTTAIEHGFFTDFANPPVTDKAQADQASFLKTTTPVLSI
                     IKAP"
     gene            3340..4569
                     /locus_tag="VF516_03595"
     CDS             3340..4569
                     /locus_tag="VF516_03595"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX8106782.1"
                     /translation="MRTLILTATILVPTVASAGGYLVPSMNPRDLALGGSAVADETGP
                     EATFLNTAGLASQEGVGVGVAGALASNHTDWSDPSLGSASLSQNSTPPAVAVSYGEKL
                     PFDQAWGVGIGFGAPGGGSLIWPDNWAGQEAVRSVKTQVFGFGGGAAFQLLPYFRFGL
                     NYVRYQATEEIHQALNFLDHTSDVGLALSGGGNSLGVSTEILPPGVPLKIGITYQHSS
                     TIGLTGHVHFADVPPAFQPMLHDQPISEDFLMPDVVQAGAAYEVQPGLKVMASYVFEH
                     WSQYKSDHFTGDGGFDTTVLRNYHNAHQFGVAGEWRHLPFLPPLTVRAGVMRNLSDQP
                     TDTLSPSLTDASRWGLSIGAGYEVMPRLRVDVGYQHQILDSVTATGMDAFPGTYKTAI
                     DFLSVGINWRTDLARNK"
     gene            complement(4587..5351)
                     /locus_tag="VF516_03600"
     CDS             complement(4587..5351)
                     /locus_tag="VF516_03600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8106783.1"
                     /translation="MSAIRIHDVHKRYGRRDVLAGVSLDLAPGARLGLIGPAASGKTV
                     LCKLVCGLEQPDRGSVEVLGECLTGKREAELGAVRRRIGMLFQSPALFDFLSVADNVA
                     FPLEQRGTLPLEAIAARVSERLRAVGLAGSEAKLTAELSGGMKKRVGIARATVADAEL
                     VIYDEPTAGLDPVTTQKIYDLMAADHARTRASVLAVSSDVTALAGFVDEIAFLYRGRL
                     IYRGPARTIADAPDPLVRQFVRGELDGPLDDAGGAP"
     gene            complement(5351..>6089)
                     /locus_tag="VF516_03605"
     CDS             complement(5351..>6089)
                     /locus_tag="VF516_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8106784.1"
                     /translation="DDATTAATAVYRAGVLLLADHQVTPAWTALWRVVTDYPDEPIAG
                     DALRALVTDGRRRDARALSDQLARLLTPLAETEVADNLLWELADLAEHELGNAPAARA
                     YYDRIATDYPRSGMRDDARWHGARISRAMHDPEGAVTRLRALLATREVALLAGSYFSI
                     WLDDAQLELGKILRDDLHDLPGAAAAFRRLPEDYPASILRDDALYELAVTLAQMGDHA
                     AACAQIARLAREFTDSKYLARGRELCP"
BASE COUNT          945 a         2252 c         2038 g          854 t
ORIGIN      
        1 ggcaacgtcc gccacatctc gctgatggat ccggccaagc cggtggtgaa gatgcagctg
       61 cccgggccgg agcgcgatgg gccggccatc accagccggc ccgatctgct cggccagtgg
      121 gtgaggaact cgtacttcga ctacgccgcc ggcgaccaga tcagcgccgc ggcggtgaca
      181 acccagacct tccaggccca gtgaccaaga ctaacctcat cgtggagagc cccatgctcc
      241 ggtatctatc tgctgccgcg ctggcactgg cgatctgcgc ctgcgagccg agcctgccgc
      301 aaaccacccc gacgccgacc gtcaccgcga tcttcgatcc gaccgcggcc cagatcccgc
      361 tgcccaacga cctggcgttc gccgcgccga tcaacagcgt gtgtccgccg ccggccaacg
      421 ccttgacggc cccgaccgat ccaccgcagt gcgcgcaggc cgagctgctc aaggccctca
      481 atccgagcgg cgtcccgctc gcgggcgcgt tccccagcga tcaggaagtc ccgctgacga
      541 tcgacttcac cgagagcacc tacgtcaaca gccgcgtcaa gcaggtcgcg ccggatctcg
      601 atctctcgac gatcacgccg gcgacgctgt tcgtgatcgg ccccgccgga ccgatcgcgc
      661 tcgagccgcc cacgccggac gactacgtcg tcccccctcc ggtgaacatg gtcccggccg
      721 accacggcac gctgagcgtg caccgccagg gccaccagcc gtggccgccc ggcgcctacg
      781 tgctggtcct gcgcggcgga gaccaggggg tcaagaccaa ggcgaccgcc gacacgcccg
      841 cgatcccggt cacgccgtcg ccggtgttcg atctgatcga gcagggcaag gacatgacgg
      901 cccccgagaa cctcgggctg ctcaaggccc agagcggtgg ctcgaccgcg gatgcgatcg
      961 cccagggcca gcagctcaac ctcttgatcg cgatctacaa ggccacggcg ttccccgccg
     1021 cggatacggt gttcccgcac gagcagctgg cgatcgccag cacgttccac atcggcccga
     1081 cggtcaccaa cgtcgaggtc gatccggcgc gcggcctggc gccgcttccg ttcgacctgc
     1141 tgcgcgacgc gggcaccggc aagctcacgc cgctggcggc ctgcacgctg gcgggctcga
     1201 gcctccgcaa ggacgggacc tgccccagcc cggccgcggc ggggttccag acgctcgacg
     1261 ggttctcgac caccggcgcg atcctcgcgc cgacgtcgga tctgatccag gcgtcgaccg
     1321 tgaccaagga cacgctgatg ctgttcgacc tgagcgatcc caagaacccg accttggtcc
     1381 ccgcgggctc gctgatcgtc gagccgtgcg agttcacctc cagctgcacc ggacccgcgc
     1441 tggcgccggt gatcgcgttc cagccggcgg gcgccacggc ggtcgacccg acgggcagcc
     1501 cgccgtcggt gttccgcacc aagccgctca aggacagcac cgactacgcg gtggtgatga
     1561 ccaccgacat caaggacaag gccggtaacg cgatcggacc gggcacggtg gccaagatcc
     1621 tgcagttcaa gaacccgctg accgtcggga ctcactcggc gctgctgggc atcgacgatg
     1681 ccaccgcggc ctcgctcgac aagatgcgca agcagctggc cccggtgttc accgcgctcg
     1741 gggcgaacgc caccaaggtc gcgatggcgt acacgttcca cacccagacc gtgctgggtc
     1801 atgcggtcga tctcgccgcg ttgccgtaca gcacaccgac ggtcacagcc gggaccggtc
     1861 cggtgacccc tcctccagtg aacccgcccg atccggcggc ggacgactcg ccgcgcaacg
     1921 cgttcaccaa gttcggcgtc attccccgca cggctccggc ggacaccacg ccgggccacg
     1981 ttccgctcgc cgacatcgac gaggtcctgg agaccacgat caccacgttc aacgcgctcg
     2041 atcccgcgac cggcgcattc cttcccgatc cgagcaaggc ggccccggag acgatcaagg
     2101 tcctgatcgc cacccccaag gcgaccgcgg ctcctgactg cccggcagcc ctgtcggcgt
     2161 tcaacaagtg cgctccgctg gtcgtgttcc gccacggcct ggggcgtggg cgtgccgaca
     2221 tgctgctgat cgccaatgcg ttcgcggcag ccggcatggt gaccgtggcg atcgacgccg
     2281 cgaagcacgg cgatcgcacg ctgtgcagct cggccgcgac gccgccgctg agcggatgcg
     2341 ccgggacctg tacaccgctc tccaatgctc cagacccgat cgatccgccg tcgcaggggc
     2401 ctcccggaac gtgcgatccc acgaagctga tcaaggcgcc ggtcgacaag aatgcgacgg
     2461 gggcgaccga tggcatcgcc gtcgcgtccg gcaattacct gatcagcgcg aatttcttcc
     2521 ggacgcgcga cacgcttcgc caggatctga tcgacgagtc gcagctgatc cgcgtggtcg
     2581 cgctcgatcc cagcaagccg ccgatgacga cgctgctgtt cgagcggatc aagagccgtg
     2641 cgatggcgaa gttcggggtc accatgatcg tcgacccgac cgccgtgtac ttctccggcc
     2701 agtcgctcgg cgcgatccag ggagcgatgg acgtcgcgac caacccgcgg atctcccggg
     2761 cggcgttcaa cgtcggcggc gggaccctcg tcgacgtgtt cgcgaccgcc ccggcgttcc
     2821 acgctgggtt cacgcagctg atcagcgcgc tcggcatcac gccgggtacg tcgcagttcc
     2881 tccagttctt gaccgtcgcc aagaccgtcc tcgatccggc cgatccgatc aacttcgtgg
     2941 gtcacctgac ggacaaggac cacatgctgc ccaacctgct gccaccgctc ggcaacccga
     3001 acgggttggt gccgcagcag cccaaggcga tcctgagcca gatcgccaac tgcgacgaca
     3061 ccgtgccgaa cccgttcaac ttcctctatg cgagcaacgc aggcttgagc ccgctaccgc
     3121 ccagcggtca gccggggacg gtgcagcagt tcgtcggcgc agggtttgac ccggcgaagc
     3181 cgacgctctg ctcgccgacc accgccatcg agcatggctt cttcaccgac ttcgcgaatc
     3241 ccccggtcac agacaaggcg caagccgatc aggcgagctt cctgaagacc accacgcctg
     3301 tcctcagcat catcaaggca ccctgaacga ggccacccca tgcgcacact gatcctcaca
     3361 gccacgatcc tggtgcccac ggtggcctcc gccggcggct acctggttcc cagcatgaac
     3421 ccccgcgacc tcgcgctcgg cgggtcggcg gtcgccgacg agaccggccc ggaggcgacg
     3481 ttcctcaaca ccgccggcct ggcgtcccag gagggcgtcg gcgtcggcgt cgccggcgcg
     3541 ctggccagca atcacaccga ctggtcggat ccgtcgctgg gctcggcgtc gctgtcgcag
     3601 aacagcacgc cgccggcggt cgcggtctcg tacggcgaga agctgccgtt cgaccaggcg
     3661 tggggcgtcg gcatcggctt cggcgcgccc ggcggcggct cgctgatctg gccggacaac
     3721 tgggccggtc aggaagcggt tcgctcggtg aagacccagg tgttcgggtt cggcggaggc
     3781 gccgcgttcc agctgctgcc gtacttccgg ttcggcctca actacgtacg gtaccaggcg
     3841 accgaggaga tccaccaggc gctcaacttc ctcgatcaca ccagcgacgt cggcctcgcg
     3901 ctgtcgggcg gcggcaacag cctcggcgtc tcgaccgaga tcctgccgcc gggggtcccg
     3961 ctcaagatcg gcatcaccta ccagcactcg tcgacgatcg gcctcaccgg ccacgtgcac
     4021 ttcgccgacg tgccgcccgc gttccagccg atgctccacg atcagccgat cagcgaggac
     4081 ttcctgatgc ccgacgtggt gcaggccggc gcggcctacg aggtccagcc cggcctcaag
     4141 gtcatggcct cgtacgtctt cgagcactgg agccagtaca agagcgatca cttcacgggc
     4201 gacggcggat tcgacaccac cgtgctgcgc aactaccaca acgcgcatca gttcggcgtc
     4261 gccggcgagt ggcgccacct gccgttcctg cccccgctga ccgtccgggc cggcgtgatg
     4321 cgcaacctct cggatcagcc caccgacacg ctctcgccgt cgctcaccga cgcgagccgc
     4381 tgggggctct cgatcggcgc gggctacgaa gtcatgccgc ggctgcgggt cgacgtcggc
     4441 taccagcacc agatcctcga ctcggtgacc gcgaccggga tggacgcgtt ccccgggacc
     4501 tacaagaccg cgatcgactt cctgtcggtc ggcatcaact ggcggacgga cctggcgcgc
     4561 aacaagtagc cgggcggcgg gccggttcag ggcgcgccgc cggcgtcgtc gagcgggccg
     4621 tcgagctcgc cgcgcacgaa ctggcggacc agcggatcgg gggcgtcggc gatcgtgcgc
     4681 gccgggccgc ggtagatcag ccggccgcgg tagaggaacg cgatctcgtc gacgaagccc
     4741 gccagcgcgg tgacgtcgga cgacaccgcg agcacgctgg cgcgggtccg cgcgtggtcg
     4801 gcggccatca gatcgtagat cttctgggtc gtgaccggat cgaggccggc ggtcggctcg
     4861 tcgtagatca ccagctcggc gtcggcgacg gtggcgcgcg cgatgccgac ccgcttcttc
     4921 atgccgcccg acagctcggc ggtcagcttg gcctcgctgc cggccaggcc gaccgcgcgc
     4981 agccgctcgc tgacccgcgc ggcgatcgcc tcgagcggca gggtgccgcg ctgctcgagc
     5041 gggaacgcga cgttgtcggc gaccgacagg aagtcgaaca gcgccgggct ctggaacagc
     5101 atgccgatcc ggcggcgcac cgcgccgagc tcggcctcgc gcttgccggt caggcactcg
     5161 ccgagcacct cgacgctgcc gcgatcgggc tgctccagcc cgcacaccag cttgcacagc
     5221 acggtcttgc cggacgccgc cgggccgatc agcccgagcc gggcgccggg cgcgagatcg
     5281 agcgacacgc cggcgagcac gtcgcgccgc ccgtagcgct tgtggacgtc gtggatccgg
     5341 atcgcgctca tcacgggcac agctcccggc cgcgcgcgag gtacttggag tccgtgaact
     5401 cgcgggccag gcgcgcgatc tgcgcgcacg ccgcggcatg atcgcccatc tgcgccaggg
     5461 tgaccgcgag ctcgtacagc gcgtcgtcgc gcaggatcga cgccgggtag tcctcgggca
     5521 accggcggaa cgcggcggcg gcgcccggca ggtcgtgcag gtcgtcgcgc aggatcttgc
     5581 cgagctcgag ctgggcgtcg tcgagccaga tcgagaagta cgagccggcg agcagcgcga
     5641 cctcgcgggt cgcgagcagc gcgcgcagcc gggtcaccgc gccctcggga tcgtgcatcg
     5701 cccgcgagat ccgtgcgccg tgccagcgcg cgtcgtcgcg catgccgctg cgcggatagt
     5761 cggtcgcgat ccggtcgtag taggcgcggg cggccggcgc gttgccgagc tcgtgctcgg
     5821 cgaggtcggc gagctcccac agcaggttgt cggcgacctc ggtctcggcg agcggggtca
     5881 agaggcgggc gagctggtcg gacagcgcgc gggcgtcgcg gcggcggccg tcggtgacca
     5941 gcgcgcgcag cgcgtcgccg gcgatcggct cgtcggggta gtcggtcacg acccgccaca
     6001 gggcggtcca cgccggggtg acctggtggt cggcgagcag cagcacgccg gcgcggtaca
     6061 ccgcggtcgc ggcggtggtg gcgtcgtcg
//
LOCUS       DASVOR010000159         3174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_235196,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000159 DASVOR010000000
VERSION     DASVOR010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235196"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF516_03610"
     CDS             complement(<1..342)
                     /locus_tag="VF516_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8106785.1"
                     /translation="MSRFHRRILWLIGIGMFFDGFDIYIMSTVLGATVASKFSTLAEN
                     AQFISMTFVGMTVGSLATGFLGDRFGRRFTYQVNLMVFGLASLAAALAPNMGVLIACR
                     FVMGVGLGAENV"
     gene            complement(428..1177)
                     /locus_tag="VF516_03615"
     CDS             complement(428..1177)
                     /locus_tag="VF516_03615"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8106786.1"
                     /translation="MSGPASTTSHGPAPGVRIDHQGDVLVMTIAHPPRNEITGAMFEA
                     MLDALRREEAQPRARVLRIRAEGEVFCLGRERAGRDEAAIRAEVTRLIALKRAVMTSP
                     LITVAEVQGDAAGFGAGLAVLCDFTVVAGTAKLAFPEMRHGLPPAAIMAYLGRYAQPK
                     RIFPLILLAPEFSAAHARDIGLVTQVARPDALRDDVDEIVRRILAMDAEAVRQCKAYF
                     QAAHGGDLDDNFARATDLLTARTMQLLARRG"
     gene            1341..>3174
                     /locus_tag="VF516_03620"
     CDS             1341..>3174
                     /locus_tag="VF516_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8106787.1"
                     /translation="MVNAGGLLYNAIWARWPTSVSMILDRRPPGRGDRRHDGPTSTAG
                     TPGKRTLTEAIAPPAPGRSPSPDVARARVQLAADAGTQGSGGALPYRDAIQRSFGRHD
                     VSQIRAHSGADAEAAAGAIGAAAFATGHHVVFAGTPSLHTAAHEAAHVVQQRAGVHLL
                     GGVGQAGDAYEQHADAVADRVVRGESSEDLLDVHAGTGAAAGGAVIQLLPSTFSARLA
                     SDPLFNLPLLKQVTQSAWNAYVIQVKQYFQVEDDDEHRQRAAFMTTYQRATKLYSDCQ
                     EEFTEAQKVLAAEIQTMLETERRQLFGGGAVAMTDPSVAVPDSELTTKDEHGPFKSRF
                     DRDDPGAWPVFAGTPTIQQVQQGNLGDCYLMAAIASIVHTNPQHFFDHILDRLDSRVT
                     VKLYDHTGAAVNVTVEKSVVGDHCAHGSLWVALLEKAYVAAGFKGSRGKETQDPSKSY
                     AAIAGGDASTAMAHLTGQKTGGLAIGAERAAVSEKVTTELRAETRRIEDAMRELDLVI
                     KTKKEQSKDTTAEDQQMNQLLEDLSKLQTPQGELFSTLTLHYVSRDSIATFLARHADK
                     PLFVAAVKRATSLDAEQGVLPGVLGSGKYGKDEIRLFEVIKQQVD"
BASE COUNT          544 a         1084 c         1130 g          416 t
ORIGIN      
        1 gacgttctcg gcgccgagcc cgacgcccat cacgaaccgg caggcgatca ggacccccat
       61 gttcggggcc agcgccgcgg ccagcgacgc cagcccgaac accatcaggt tgacctggta
      121 ggtgaaccgc cggccgaacc ggtcgccgag gaagccggtg gcgagcgagc cgaccgtcat
      181 gccgacgaac gtcatcgaga tgaactgcgc gttctcggcc agggtcgaga acttgctggc
      241 gaccgtcgcg cccagcacgg tcgacatgat gtagatgtcg aacccgtcga agaacatccc
      301 gatcccgatc agccacagga tccggcgatg aaacctcgac atcggcaagc ggtccagtcg
      361 tgcgcctgca ttcacagtca tcgcattgcc tccgccgaaa gggaatggaa cgggagcgaa
      421 acggggccta gccgcgccgc gcgagcagct gcatggtgcg cgcggtgagc agatcggtgg
      481 ctcgcgcgaa gttgtcgtcg agatcgccgc cgtgcgcggc ctggaagtac gccttgcact
      541 gccgcaccgc ctcggcgtcc atcgcgagga tgcgccggac gatctcgtcg acgtcatcgc
      601 gcagcgcgtc gggccgcgcg acctgggtga ccagcccgat gtcgcgcgcg tgcgcggcgg
      661 agaactccgg cgcgagcagg atcagcggga agatccgctt gggctgcgcg tagcgcccga
      721 ggtaggccat gatcgccgcg ggcggcaggc cgtggcgcat ctcgggaaac gccagcttcg
      781 cggtcccggc gaccacggtg aagtcgcaca ggacggcgag cccggcgccg aaccccgccg
      841 cgtcgccctg gacctcggcc accgtgatca gcgggctggt catcaccgcg cgcttgagcg
      901 cgatcaaccg cgtgacctcg gcgcggatcg ccgcctcgtc gcggcccgcc cgctcgcggc
      961 cgaggcaaaa cacctcgccc tcggcccgga tccggagcac ccgcgcccgc ggctgggcct
     1021 cctcgcggcg gagcgcgtcg agcatcgcct cgaacatcgc gccggtgatc tcgttgcgcg
     1081 gcggatgggc gatcgtcatc accagcacgt cgccctggtg atcgatgcga acacccggcg
     1141 ccgggccatg gctggtcgtg ctcgcgggcc cgctcatcgc tgcggagtct atgcgtcgcg
     1201 cggtgcagag cgcaagactg catggtgcgg tgcgcccggc cggtgcagcc gcgtcagacg
     1261 gccgatctgc gctggtcggt ccacctggct tcgtgccggc gcggtggtgg cggcccgcgg
     1321 gcctgcggta atcggccgta atggtcaacg ccggcgggct gctctacaac gcgatctggg
     1381 cccggtggcc cacgagcgta tccatgattc ttgatcgacg cccgcccggc cgtggcgacc
     1441 gccgccacga tggcccgacg agcaccgccg gcacgcccgg caagcgtacg ctgaccgaag
     1501 cgatcgcacc gccggccccg ggccggtcgc cgtcccccga tgtcgcgcgc gcgcgcgtgc
     1561 agctcgcggc agatgccggg acccagggca gcggcggggc gctgccgtat cgcgatgcga
     1621 tccagaggtc gttcggcagg catgacgtgt cgcagatccg cgcgcacagc ggagcggacg
     1681 ccgaggccgc cgccggggcg atcggcgccg cggcattcgc gaccggacat catgtggtgt
     1741 tcgcagggac accgagcctg cacaccgcgg cgcacgaggc cgcgcacgtc gtgcagcagc
     1801 gcgccggtgt gcacctgctg ggcggcgtcg gccaggccgg cgacgcgtac gagcagcatg
     1861 cggacgcggt ggccgatcgg gtggtgagag gcgagtcgag tgaagacctg ctcgatgtcc
     1921 atgcagggac gggcgcggcg gcggggggcg ccgtcatcca gctcctcccc tcgacgttca
     1981 gcgcgcgcct ggccagcgac ccgctgttca acctgccgct gctcaagcag gtgacgcagt
     2041 cggcgtggaa tgcgtacgtc atccaggtca agcagtactt ccaggtcgag gacgacgacg
     2101 agcaccgcca gcgcgcggcg ttcatgacga cctaccagcg cgcgacgaag ctatactccg
     2161 attgccagga ggagttcacc gaggcgcaga aggtactggc ggcggagatc cagacgatgc
     2221 tggagaccga gcgcaggcag ctgttcggcg gcggcgccgt ggcgatgacg gacccgagcg
     2281 tcgcagtccc cgactcggag ctgacgacca aggacgaaca cggcccgttc aagagccggt
     2341 tcgacaggga cgatcccgga gcctggccgg tgttcgcagg cacgcccacg atccagcagg
     2401 tccagcaggg caacctgggc gattgctatc tcatggcggc gatcgcgagc atcgtccaca
     2461 ccaacccgca gcacttcttc gaccacatcc tcgatcgcct cgatagccgg gtcacggtca
     2521 agctgtacga ccataccggg gccgccgtga acgtcacggt cgagaagtcg gtggtcggcg
     2581 accactgcgc ccacggcagc ctgtgggtcg ccctgctgga gaaggcgtac gtcgcggcgg
     2641 gcttcaaggg gtccaggggc aaggagacgc aggatccgag caagagctat gccgcgatcg
     2701 cgggcggaga cgccagcacg gcgatggccc acttgaccgg gcagaagacc ggtggcctcg
     2761 cgatcggagc ggagcgcgcc gcggtgagcg agaaggtcac cacggagctc cgggccgaga
     2821 cgcgtcgcat cgaggacgcg atgcgcgagc tcgatctcgt gatcaagacc aagaaggagc
     2881 agtcgaagga caccacggcc gaggaccagc aaatgaatca gctgctcgag gacctgagca
     2941 agctgcagac cccgcagggc gagctgttct cgacgctgac gctgcactac gtcagccgcg
     3001 actcgatcgc gacattcctc gccaggcacg ccgacaagcc gctgttcgtc gcggcggtca
     3061 agcgagccac ctcgctcgac gcagagcagg gcgtgctgcc cggcgtgctg ggcagcggga
     3121 agtacggcaa ggacgagatc cggctgttcg aggtgatcaa gcagcaggtc gaca
//
LOCUS       DASVOR010000160         2624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_264411,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000160 DASVOR010000000
VERSION     DASVOR010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264411"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..855
                     /locus_tag="VF516_03625"
     CDS             118..855
                     /locus_tag="VF516_03625"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrsF family protein"
                     /protein_id="HEX8106788.1"
                     /translation="MSALEPGAEAGAEAGIEPGLEDLARRPAPRPPALSPALEAELAQ
                     LTPVATRRPLRQLALLIAISLIYGAGLVVMLTVRRDLHELPMGWLVSTGLAWLLGFVL
                     PITLATVPRAGSVMPRWRLAGIASVVAAIAMIGLGLVLHPAGEHSAALGWDGVVRGYG
                     CLAHGLETAIVPVVIGTIFLRRVLPVGSRWVAAGLGAGGGSLGGLVLHLHCPIADGLH
                     VGLVHGGVIAIAALLAAALMPRAIELR"
     gene            903..1598
                     /locus_tag="VF516_03630"
     CDS             903..1598
                     /locus_tag="VF516_03630"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEX8106789.1"
                     /translation="MIGLVLSCEHASWTLPPGVDLGVPLDVLRSQASWDHGALDIATR
                     LSEAVGLPVHASAFSRMFVDLNRPADHPNVIPQVSYGAVVPGNAGLAPGDRQARIDGF
                     HTPYWDAVRRDVTARIQDRGRVLHFCSHTFDPALDPAQRTYDVGVLYDPGHALEAQLA
                     ERLMFQIRRAGLDVRANQPYPGTGPAICTTLRHELGAPYAGIQLETSHAVTYRAGGCA
                     RVADVIAEFFETL"
     gene            complement(1617..2240)
                     /locus_tag="VF516_03635"
     CDS             complement(1617..2240)
                     /locus_tag="VF516_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106790.1"
                     /translation="MLRVLSLAAVLAMASPASADVLKLFVEANGGGVAGTATGGDPVV
                     KDAAFFAKAPHLAYGALVGAEFLFLDGWIQHHQFTDGSRIATWTQFGVGVHGTIDTGD
                     PKQQKAHEGGYVEFGVGGWFGLGTGQQVMPPLDNAQITDKAFLLEGRVGAGTHLSSVF
                     DLGVTVPVSWGYFLKNGNGAAANNTSTHYRSVQGEALVVLRANIRLL"
     gene            2311..>2624
                     /locus_tag="VF516_03640"
     CDS             2311..>2624
                     /locus_tag="VF516_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106791.1"
                     /translation="MRIRLAILVTLGACLGACLGACLGACKAHDVDRLTEIKRDVCAC
                     KDAKCADQAISGVPKASIRSTPRTQAIARDILDCRARLEAADRPSTDPDTEGGSDGSG
                     ELG"
BASE COUNT          323 a          975 c          956 g          370 t
ORIGIN      
        1 cacggtgggc gcgatgaagg tgcgcgcgca ccgcggctac gaggcgctgc gcaagctgct
       61 cggcaaggcg ccggccgtcc cgccggccaa gcccgccgcc ggggccggag gtggatcgtg
      121 agcgcgctcg aaccgggtgc cgaagcgggt gccgaagctg gaatcgagcc ggggctcgag
      181 gatctcgccc ggcggccggc gcccaggccg cctgcgctgt ccccggcgct ggaggccgag
      241 ctcgcacagc tgaccccggt cgcgacccgg cggccgctgc gccagctcgc gctgctgatc
      301 gcgatctcgc tgatctacgg cgccggcctc gtcgtgatgc tcacggtgcg ccgcgacctc
      361 cacgagctgc cgatgggctg gctggtgtcg accggcctgg cctggctgct cggcttcgtc
      421 ctgccgatca cgctggcgac cgtgccgcgc gccggctcgg tgatgccgcg ctggcggctg
      481 gcgggcatcg cgtcggtggt cgccgcgatc gcgatgatcg gcctggggct ggtgctgcat
      541 ccggccggcg agcacagcgc ggcgctcggc tgggacggcg tggtgcgcgg ctacggctgc
      601 ctggcccacg ggctggagac cgcgatcgtg ccggtcgtga tcgggaccat cttcctgcgc
      661 cgcgtgctgc cggtgggctc gcgctgggtc gcggcggggc tcggcgccgg cggcggcagc
      721 ctcggcggcc tcgtgctcca cctgcactgc ccgatcgccg acgggctgca cgtcgggctg
      781 gtccacggcg gcgtgatcgc gatcgccgcg ctgctcgccg ccgcgctgat gccgcgggcg
      841 atcgagctgc ggtagcgccg ccgcactggc acggcgcccg cttgcgtgta cactgcgtgc
      901 ccgtgatcgg cctggtgctg tcctgcgagc acgcatcgtg gacactgccg cccggggtcg
      961 atctgggggt cccgctcgac gtgctgcgct cgcaggcgag ctgggaccac ggcgcgctcg
     1021 acatcgcgac ccggctgtcc gaggcggtcg gcctgccggt ccacgccagc gcgttctcgc
     1081 ggatgttcgt cgacctcaac cgcccggccg accaccccaa cgtgatcccg caggtcagct
     1141 acggcgcggt ggtgcccggc aacgcgggcc tcgcacccgg cgaccgccag gcccggatcg
     1201 acggcttcca cacgccgtac tgggacgcgg tgcgccgcga cgtgaccgcg cggatccagg
     1261 accgcggccg cgtgctgcac ttctgctccc acaccttcga ccccgcgctc gacccggcgc
     1321 agcgcaccta cgacgtcggc gtgctctacg acccggggca cgcgctcgag gcccagctgg
     1381 ccgagcgcct gatgttccag atccgccgcg ccgggctcga cgtccgcgcc aaccagccct
     1441 accccggcac cggccccgcg atctgcacga cgctgcgcca cgagctcggc gcgccctacg
     1501 ccgggatcca gctcgagacc tcgcacgcgg tgacctaccg cgccggcggc tgcgcgcggg
     1561 tcgccgacgt gatcgccgag ttcttcgaga ccctgtagcg accgccggcg ctacagctag
     1621 agcaggcgga tgttggcgcg cagcacgacc agcgcctcac cctggacgct gcggtaatgg
     1681 gtcgaggtgt tgttggcggc cgccccgttg ccgttcttga ggaagtagcc ccacgacacc
     1741 ggcacggtga cgccgaggtc gaacacgctg ctcaggtgcg tgccggcgcc gacccggccc
     1801 tcgagcagga acgccttgtc ggtgatctgc gcgttgtcga gcggcggcat cacctgctgg
     1861 ccggtgccca ggccgaacca gccgccgacc ccgaactcga cgtagccgcc ctcgtgggcc
     1921 ttctgctgct tgggatcgcc ggtgtcgatc gtgccgtgga cgccgacccc gaactgggtc
     1981 caggtcgcga tccggctgcc gtcggtgaac tggtggtgct ggatccagcc gtcgaggaac
     2041 aggaactccg cgccgaccag cgcgccgtac gccaggtgcg gcgccttggc gaagaacgcc
     2101 gcgtccttca cgaccggatc gccgccggtc gccgtgccgg ccacgccgcc cccgttggcc
     2161 tccacgaaca gcttgagcac gtccgccgac gcgggagagg ccatggcgag gacggcggcg
     2221 aggctcagga cgcgcagcat cgcgctattg tcgccggttt cggcggtacg cgccaatggc
     2281 gtggcgcagc tcacaggcta tgctgcgacg gtgcgtatcc ggctggcgat cctggtcacg
     2341 ctcggtgctt gcctcggtgc ttgcctcggt gcctgcctcg gtgcctgcaa agctcacgac
     2401 gtcgaccgcc tgaccgagat caagcgcgac gtgtgcgcct gcaaggacgc gaagtgcgcc
     2461 gatcaggcga tcagcggggt gccgaaggcg tcgatccggt cgacgccgcg cacgcaggcg
     2521 atcgcgcgcg acatcctgga ctgccgcgcc cgcctcgagg ccgccgaccg gccgagcacc
     2581 gatccggaca ccgagggcgg cagcgacggc agcggcgagc tggg
//
LOCUS       DASVOR010000161         7583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_498370,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000161 DASVOR010000000
VERSION     DASVOR010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7583
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498370"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            495..3611
                     /locus_tag="VF516_03645"
     CDS             495..3611
                     /locus_tag="VF516_03645"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8106792.1"
                     /translation="MRAVRSEGEARDPVAVVGIGCRFPGARGPRQFWKLLRTGGDAIT
                     EVPGGRFDLERLYDPTPATPGKLSSRFGGFIDGVDEFDPEFFSISPREARHMDPQQRL
                     ALEVAWEALEDAGTVATALRGSAAGVFLGMQSNEYQELEHRFLPEVDLAVATGGSRNG
                     AAARISHMLGIEGPSIVLDTDRASSLVAVHFACQSLWSGESDLALAGGTNLVLSPEPS
                     LALSRAGMLARDGRCKFCDARADGIVRSDGVGIVVLKRLRDAVAARDRVYAVILGTAI
                     NHNGGRSGDLMTPSRDSQAAVVRSACRAAGVAGRDVQYVEAHGTGTVVGDPIEVEALA
                     LALRSGDDATAPPLRVGSVKTNIGHAEAAAGIAGLIKVALAVRHGALPASLHCQVPNP
                     AIRFDGKVLVQTETAGWPDPAPRLIAGVTSIGLTGTNAHAVLAAPPPPSAPLDVVPRH
                     EVLPLSAASRDSLIELARRYHEVLSAAAPGAPVIDLAYTAAVRRTHHPERLAFIGANR
                     DELLRAVETFIRTSDASAWTLGPAQRRVKIAFLFSGHGSQWIGMGRELLASEPVFRDT
                     LAAWDKAVQRLFGWSVLEELARPAPASRLDEIEITQPVLVAVQVALAAQWRAWGVVPD
                     WVAGVSMGEVAAAHVAGILSLDDAVRIICERARLMKQLAGKGTMALVELPLSEAERLI
                     RPRAHEVSIAGSYSAGATLLSGAPRVLEDVLQALHSKPVFFRNVKVEVASHSPQMDAI
                     RAPLIEAVAGIAPAPARMPMISTVTTAQLAGPDCDPAYWGRNLRDPVLFAAALRQLAE
                     SGCNVFVEMSPHPTLLGHVLDGLAGLKRQGLALGSLKRAEPERQTLLRSLGSLYTYGY
                     DVAWDRVFGDDGHMIDAPTYAWQRARYWFAEGAVANPPATAAALATVDGEWLARLARL
                     PTAERLAQAVELVCIEVAHVLSLEHEARVTADRPLRELGLGSALALELRHALRMRTGV
                     ELSATFVFDYPTPLAMASYLVQGAAPVNGAAHATRALEPETVSLAELPDRELAALAER
                     LIDGGDDR"
     gene            3622..5004
                     /locus_tag="VF516_03650"
     CDS             3622..5004
                     /locus_tag="VF516_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEX8106793.1"
                     /translation="MADSSDASLRPALEKSIREIQRLKGELGRLRGEPIAILSMAARL
                     PGGVRAPEDLWRLLDEGRDAIGPFPDSRGWDLEQLYSPEASGPGKTATNQGGFLYDAE
                     LFDPMFFDISPRETERIDPQQRLVLECAWEALERALIVPASLFQTATGVFVGCGYSDY
                     GGRMLPSPETFDGYVFTGSSGSTASGRVSYTLGLRGPAITIDTACSSSLVSIHLACAS
                     LRSGECDLALAGGVTVMATPMLFLDFSRQGGLAPDGRIRAFGAGANGTAWGEGCGILA
                     LKRLSDAQRDGDPIVAVIRGSAVNQDGRSQGFTAPNGPSQELVIRQALAAAGLTAADV
                     DVVEAHGTGTRLGDPIEAGALLATYGQEHTRERPLWLGSIKSNIAHTQWAAGVAGMMK
                     LVLAMQHQRIPKTLYADPPSPHVDWNRGHVVLAREPVPWPRGERRRRAAVSSFGISGT
                     NAHLILEQAP"
     gene            5001..>7583
                     /locus_tag="VF516_03655"
     CDS             5001..>7583
                     /locus_tag="VF516_03655"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8106794.1"
                     /translation="MTEPVERPLHVLPLAARSERALAELAGRYAGALGVSPVSFANIC
                     HTAGVARTAFEERLALVAADARDARDKLAAFAAGATPQAIVRGRADGAPKIAFMFTGQ
                     GSQWSGMGKALYDTHPAFRATLDECAGIVGDALARPLLDMMFDPTCGELLGQTANTQV
                     ALFALEFALWTLWRSWGITPAVLVGHSVGEYVAACAACVFSLEDGLRLMVERGRLMQA
                     CAEGEMASIAAPLAAVEAAVAPHAARASVAAHNGKEQVVSGHADAVRAICAAFETEGH
                     LSRKLAVTRAFHSPLTDPILGDLERLLAGMRLRAPELPIVANLTGEPVGAELATARYW
                     CDQARRAVHFAEAMRVLEDKQIDLFLELGPQPTLAAMGASCVKPGFGEWLPSLRRGRD
                     AWATMLGALGRLYVRGARIDWREVDRPYSRSRVAIPVYPWQRERYWMDEPRRRSPRKL
                     HPMLPEATPLATAEPAHVWEVSFDSPELAYLQDHVVNETVILPAAGFIELALAAARHC
                     LGDGAREIKELALHEALVLLPGQPCTVQVVMAREGSCYAFRVATHIDGTWKRHVTAKV
                     RMHAAPPPPDEPLAPPLRLGKLEAAAFYELLSTKVKLNSGPAFLGVRELWTAPGEVVA
                     AIQLAREAGAPRSYEVHPALLDACLQTAFALAPLAEGVTVPMSIDAVRFHASPGAAVR
                     VHARLRAGEPPTAHGGVADLVVSGDNGRVLVEVDGFRWRRFGTGAASDTSFLVTEWRP
                     TELAAPGAAPRGRWLLLSDRGSCGAALRARLEAGGADVAEVHVADVEVTSPAAFDAVL
                     QRHAPERIVYLWALDTPGDAMTVHSVQDACVREAAALLHLVKALGRRGGELPRLAFVT
                     RGAQA"
BASE COUNT          998 a         2743 c         2714 g         1128 t
ORIGIN      
        1 atgcccatcg ggccgcgggg catgccgggc ccgggagcca tgcccggcgg cgccatcagg
       61 ttggtcgcgc attgagagca gaacgcggcg ttgggcaggt tctgggcgcc gcaagtcgga
      121 cagttcatca cacaccccct ctctccatcc tgaccgcttg gtgcgccgaa atcaaccgcg
      181 accggcatgg cgatcgcgcg cgcgtgatgc gtgaagcagg gatggatcct gcagtgacca
      241 cacaggcttg cgccggagcc gcctcccggt tcgtcctccc agatacctcg cctgcccgcg
      301 aggccggacg acgagcttcg cgacatcgcg gtcgtgcgga tgcctccgcg agagcgacga
      361 cctcgatgcg ggctcgcccc atccgcgacc ggtccggctc tcgcttccac gccgtcgtac
      421 accagcacgg cgagcacctc gctggtcgat cactgcaggg actgacgaac gccgcatgtt
      481 aacatggtcg gacgatgcgg gcggttcgca gcgagggtga agctcgcgat ccggtcgccg
      541 tcgtgggcat cggctgccgg ttccccgggg cgcgtggccc ccggcaattc tggaagctgc
      601 tccgcaccgg cggcgacgcg atcaccgagg tgcccggcgg ccggttcgat ctcgagcggc
      661 tctacgatcc cacgcccgca acgccgggca agctgtcgtc gcgcttcggc ggcttcatcg
      721 acggcgtcga cgagttcgat cccgagttct tctcgatctc gccgcgcgag gcgcgccaca
      781 tggatcccca gcagaggctc gcgctcgagg tcgcgtggga ggcgctcgag gacgccggca
      841 cggtcgcgac cgcgctgcgc ggctcggcgg cgggcgtctt cctcgggatg cagtccaacg
      901 agtaccagga gctcgagcat cgcttcttgc ccgaggtcga tctcgcagtc gcgaccggcg
      961 gctcgcgcaa cggcgccgcg gcccgcatct cccacatgct cggcatcgaa ggcccgagca
     1021 tcgtgctcga caccgatcgc gcgtcctcgc tcgtcgccgt ccacttcgcc tgccagagcc
     1081 tgtggagcgg cgagtcggat ctcgcgctcg cgggcggcac gaatctcgtg ctgtcacccg
     1141 agcccagcct ggcgttgtcg cgcgcgggca tgctcgcgcg cgacggtcgc tgcaagttct
     1201 gcgacgcacg cgcggacggc atcgtgcgca gcgacggggt cggcatcgtc gtgctgaagc
     1261 gcctgcgcga cgccgtcgcc gcgcgcgatc gcgtctacgc cgtgatcctc gggaccgcga
     1321 tcaaccacaa cggcgggcgt agcggcgatc tcatgacccc gagccgcgac agccaggccg
     1381 ccgtggttcg cagcgcgtgc cgggccgccg gtgtcgcggg ccgcgacgtc cagtacgtcg
     1441 aggcgcacgg caccgggacg gtcgtcggcg atccgatcga ggtcgaggcg ctcgcgcttg
     1501 ccctgcgctc cggtgacgat gcgacggcgc cgcctctgcg cgtcgggtcg gtgaagacga
     1561 acatcggcca cgccgaggcc gccgccggga tcgccgggtt gatcaaggtc gcgctcgcgg
     1621 tccgtcacgg cgcgttgccg gcgagcttgc actgccaggt cccgaacccc gcgatccggt
     1681 tcgacggcaa ggtcctcgtg cagaccgaaa ccgccggctg gccggacccc gcgccgcggc
     1741 tgatcgctgg cgtgacctcg atcggactca ccggcacgaa cgcccacgcg gtgctcgccg
     1801 cgccaccacc gccgtccgct ccgttggacg tcgttccccg ccacgaggtg ttgccgctgt
     1861 cggcagcaag ccgcgactcg ttgatcgagc tcgcgcgccg ctaccacgag gtcctgtctg
     1921 ccgccgcgcc cggagcgccg gtgatcgatc tcgcgtacac cgcggcggtg cggcgcacgc
     1981 accatcccga acgcctcgcg ttcatcggcg ccaaccgcga cgagctcttg cgtgcggtcg
     2041 agaccttcat ccgcacgtcc gacgcgagcg cctggacgct cggtccggcg cagcgccgcg
     2101 tgaagatcgc gttcttgttc tccggccacg gctcgcagtg gatcggtatg ggccgcgagc
     2161 tgctcgcgag cgaaccggtg tttcgagata cgctcgcggc gtgggacaag gccgtgcagc
     2221 ggctgttcgg ctggtccgtg ctcgaggagc tcgcccggcc ggcgccggcg tcgcggctcg
     2281 acgagatcga gatcacgcag ccggtgctgg tcgccgtgca agtcgcgctc gccgcgcagt
     2341 ggcgcgcgtg gggggtcgtg cccgactggg tcgccggcgt cagcatgggc gaggtcgccg
     2401 ccgcgcacgt cgcggggatc ctgtcgctcg acgacgcggt ccggatcatc tgcgagcgtg
     2461 cgcgcctgat gaagcagctc gccggcaagg gcacgatggc gctcgtcgag ctgccgctct
     2521 ccgaggccga gcggttgatc cgcccgcgcg cgcacgaggt ctcgatcgcg ggcagctaca
     2581 gcgcaggcgc gacgctgctc tccggcgccc cgcgcgtgct cgaggacgtg ctgcaagcgc
     2641 tgcacagcaa accggtgttc ttccgcaacg tgaaggtcga ggtcgcgtcg cacagcccgc
     2701 agatggacgc gatccgcgcg ccgctgatcg aagcggtcgc gggcatcgcg ccggcaccgg
     2761 cgcgcatgcc gatgatctcg acggtgacga ccgcacagct cgccggtccc gattgcgacc
     2821 ccgcgtactg gggccgtaac ctgcgcgacc ccgtgctgtt cgccgccgcg ctgcgccagc
     2881 tcgccgagtc tggctgcaac gtgttcgtcg agatgagtcc gcacccgacg ctgctcgggc
     2941 acgtgctcga cggcctcgcc gggctgaagc gccagggcct cgcgctcggc tcgctgaagc
     3001 gcgcagagcc cgagcggcag accctgctgc gctcgctcgg ctcgctctac acgtacggct
     3061 acgacgtcgc gtgggaccgc gtgttcgggg acgacggtca catgatcgac gcaccgacct
     3121 acgcgtggca gcgcgcacgc tactggtttg ccgagggcgc ggtcgcgaac ccgccggcga
     3181 ccgccgccgc gctcgcgacc gtcgatggcg agtggcttgc ccggctcgca cggctaccca
     3241 ccgccgaacg cctcgcgcag gcggtcgagc tcgtgtgcat cgaggtcgcg cacgtgctgt
     3301 cgctcgagca cgaggcgagg gtcaccgcag atcgcccgct tcgcgagctt ggcctcggct
     3361 ccgcgctcgc gctcgagctg cgccacgcgc tgcggatgcg caccggcgtc gagctgtcgg
     3421 cgacgttcgt gttcgactac ccgacgccgc tcgcgatggc gtcgtacctg gtccagggcg
     3481 cggctccggt caacggcgct gcgcacgcga cacgcgcgct ggagcccgag acggtctcgc
     3541 tcgcggagct tcccgaccgc gagctcgccg cccttgccga gcggctgatc gatggcggcg
     3601 acgaccgctg aaaggactgc gatggccgac tccagcgacg ccagcttgcg tcccgcgctc
     3661 gagaagagca ttcgcgagat ccagcgcctc aagggcgagc tcggccgcct gcgcggcgag
     3721 ccgatcgcga tcctgtcgat ggcggcgcgc ctgcctggtg gcgtacgcgc gcccgaggat
     3781 ctgtggcgcc tgctcgacga gggccgcgat gcgatcgggc cgttccccga cagtagaggc
     3841 tgggacctcg agcagctgta ctcgccggag gccagcggcc ccggcaagac cgcgaccaac
     3901 cagggcggct tcctctacga cgccgagctg ttcgatccga tgttcttcga catcagcccg
     3961 cgcgagaccg agcgcatcga tccgcagcag cggctcgtgc tcgagtgcgc gtgggaggcg
     4021 ctcgagcggg cgctgatcgt acccgcgagc ctgttccaga cggcaaccgg cgtgttcgtc
     4081 ggctgcggct actcggacta cggaggccgc atgctgccga gccccgagac attcgacggc
     4141 tacgtgttca ctggcagctc ggggagcacc gcgtcggggc gggtgtcata cacgctcggc
     4201 ttgcgcgggc ccgcgatcac gatcgatacg gcgtgctcgt cgtcgctggt gtcgatccac
     4261 ctcgcgtgtg cgtcgctgcg ctcgggcgag tgcgacctcg cgctggccgg cggcgtgacc
     4321 gtcatggcga cgccgatgct gttcctcgac ttcagccgcc aaggtgggct cgcgcccgat
     4381 ggccgcatca gggcgttcgg cgccggcgcg aacgggacgg catggggcga aggctgcggg
     4441 atcctcgcgc tgaagcggtt gtccgatgcg cagcgcgacg gtgatccgat cgtcgcggtg
     4501 atccgcggtt cggccgtcaa ccaggacgga cgcagccagg ggttcaccgc gccgaatggt
     4561 ccgagccagg agctcgtgat ccggcaggcg ctcgcggccg ccgggctgac cgccgccgat
     4621 gtcgacgtcg tcgaggcgca tggcaccggc acccggctcg gcgacccgat cgaggccggc
     4681 gcgctgctcg cgacctatgg ccaggagcac acgcgcgagc ggccactgtg gctcgggtcg
     4741 atcaagtcga acatcgccca cacgcagtgg gccgccggag tcgccggcat gatgaagctc
     4801 gtgctggcca tgcaacacca gcgcatcccg aagacgctct acgccgatcc gccgagcccg
     4861 catgtcgact ggaaccgcgg ccacgtcgtg ctcgcccgcg agcccgtgcc gtggccgcga
     4921 ggcgagcggc ggcgccgcgc cgcggtgtcg tcgttcggca tcagcgggac gaatgcccac
     4981 ctgatcctcg agcaggcgcc atgacggagc ctgtcgagcg tccactccat gtcttgccgc
     5041 tcgccgcgcg ctccgagcgt gcgctcgccg agcttgccgg gcgctatgcg ggagccctcg
     5101 gcgtgtcgcc ggtatcgttc gcaaacatct gccacacggc gggagtcgcg cgcaccgcat
     5161 tcgaggagcg gctggccctc gtcgccgccg acgcgcgcga cgcgcgcgac aagctcgcgg
     5221 cgttcgccgc cggggcgacg ccgcaggcga tcgtgcgcgg acgggccgac ggcgcgccga
     5281 agatcgcgtt catgttcacc ggccagggct cgcagtggtc ggggatgggc aaggcgctct
     5341 acgacacgca cccggcgttc cgcgccacgc tcgacgaatg cgcgggcatc gtcggtgacg
     5401 cgctcgcgcg cccgctgctc gacatgatgt tcgaccccac ctgtggcgag ctgctcggcc
     5461 agaccgcgaa cacgcaggtc gcgctgttcg cgctcgagtt tgcgctgtgg acgctgtggc
     5521 gctcatgggg catcaccccc gcggtgctcg tcggtcacag cgtcggtgag tacgtcgccg
     5581 cgtgcgcggc ctgcgtgttc tcgctcgaag acggcctgcg cctcatggtc gagcgcggcc
     5641 ggctcatgca agcctgcgcg gaaggcgaga tggcatcgat cgcagcgccg ctcgccgcgg
     5701 tggaggcggc ggtcgcgccg cacgccgctc gcgcgtcggt cgccgcgcac aatggcaagg
     5761 agcaagtggt ctcggggcat gccgacgcgg tccgcgcgat ctgtgccgcg ttcgagaccg
     5821 agggtcacct gagccgcaag ctcgcggtga cgcgagcgtt ccactctccg ctgaccgatc
     5881 cgatcctcgg cgacctcgag cggttgctcg ccggcatgcg gctgcgcgcg cccgagctgc
     5941 cgatcgtcgc gaacctcacc ggcgagccgg tcggcgccga gctcgcgacc gcgcgctact
     6001 ggtgcgacca ggcgcgcaga gccgtgcact tcgccgaggc gatgcgagtg ctcgaggaca
     6061 agcagatcga tctgttcctc gagctcgggc cgcagcccac gctcgcggcg atgggtgcga
     6121 gttgcgtgaa gcccggcttc ggcgagtggt tgccgtcctt gcgccgcggg cgcgacgcgt
     6181 gggcgacgat gctcggcgcc ctcggccggc tgtacgtgag gggcgcgcgc atcgactggc
     6241 gcgaggtcga tcgcccgtac tcgcgatccc gcgtcgcgat tccggtctat ccgtggcagc
     6301 gggagcgcta ctggatggac gagccgcgcc ggcgttcgcc gcgcaagctc cacccgatgt
     6361 tgcccgaggc aacgccgctc gcgaccgcgg agcctgccca tgtctgggag gtgtcgttcg
     6421 actcgcccga gctggcgtac ctgcaggacc acgtggtcaa cgagacggtg atcctgccgg
     6481 cggcgggttt catcgagctc gcgctggccg ccgcgcgcca ttgcctcggc gatggcgcgc
     6541 gagagatcaa ggagctcgcg ctccacgagg ccctcgtgct gctccccggc cagccgtgca
     6601 cggtgcaggt cgtcatggcc cgcgagggct cgtgctacgc gttccgtgtc gcgacccaca
     6661 tcgacggaac gtggaagcgt cacgtcaccg cgaaggtgcg catgcacgcc gctccgccgc
     6721 cgcccgacga gccgctcgct ccgccgctgc ggctcggcaa gctcgaggcc gcggcgttct
     6781 acgagctctt gtccaccaag gtaaagctca actccggccc ggcgtttctc ggcgtgcgcg
     6841 agctgtggac cgcgcccggc gaggtggtcg cggccatcca gctcgcgcgt gaagccggcg
     6901 cgccgcgcag ctacgaggtt caccccgcgc tgctcgacgc gtgtctgcag accgcgttcg
     6961 cgctcgcacc gctcgccgaa ggcgtgacgg tgccgatgtc gatcgacgcc gtccgcttcc
     7021 acgcgagccc cggcgcggcg gtgcgcgtgc acgcgcgact gcgcgccggc gagccaccga
     7081 ccgcgcatgg cggagttgcc gacctcgtgg tcagcggcga caacgggcgc gtgctcgtcg
     7141 aggtcgacgg cttccgctgg cgccgtttcg gcacgggcgc cgcgagcgat acgtcgttcc
     7201 tcgtcaccga gtggcgcccg accgagctcg ccgcgccggg ggccgctccc cgcgggcgct
     7261 ggctcctgct ctccgaccgt ggctcgtgcg gtgcggcgct gcgggcgcgc ctcgaagccg
     7321 gcggagcgga cgttgccgag gtccatgtcg ctgacgtcga ggtgacctcg ccagcggcgt
     7381 tcgacgctgt cctccagcgt cacgcgccgg agcggatcgt gtacctgtgg gccctcgaca
     7441 cgccaggcga cgcgatgacc gtgcactcgg tgcaggacgc ctgcgtacgc gaagcggcgg
     7501 cgctgcttca cctcgtcaag gctctcggcc ggcgcggtgg cgagctgccg aggctggcgt
     7561 tcgtcacgcg tggcgcacag gcg
//
LOCUS       DASVOR010000162         2637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_264421,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000162 DASVOR010000000
VERSION     DASVOR010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264421"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VF516_03660"
     CDS             <1..253
                     /locus_tag="VF516_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106795.1"
                     /translation="GARVVSLGERTARAGAAPAAPSSASGAVELRIELAPQVVLSLSV
                     IDDSVAVSPADVRAIRAAAAPLLAELASRRLASHPGGDE"
     gene            274..>2637
                     /locus_tag="VF516_03665"
     CDS             274..>2637
                     /locus_tag="VF516_03665"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX8106796.1"
                     /translation="MSDEEVARYGRNLDAGFGVLQTARGTLPLVAMDVDARVAGVIAT
                     IELAQRFVNTTGGAIEATYIFPLPDRAAVHRFRMEVAGRVIDGVIDERGAARAQYDLA
                     IAAGHRAAITEEERPGVFTLRVGNLMPGEAATVRLSLVGPLPVDDGEVTFRFPLVVAP
                     RFIPGAELGGDQAGLGSAADTDLVPDASRISPPVRLPGCPNPVRLGLRVALEDGVMRD
                     VASSLHAVTMARRDAQVIEVQPGERLDRDFLLRWRFGIDGAALASSLVCADDPGGAAG
                     TFALTIVPPSTRAVGAKPRDVVFVIDRSGSMAGWKMVAARRAAARMIDTLTSRDRFCA
                     LAFDNTVDALPEPGLIDATDRQRFRAVEALARVEARGGTEIAQPLGRALEMLGGGTDD
                     RERVIVLVTDGQVGNEDDVLRTVAPSLRNIKVFTLGIDQAVNAAFLRRLAAAGGGLCE
                     LVESEDRLDAVMAKVHRRIGTPIATELALRATGLELERGTLAPGKLPDVYAGAPVVIF
                     GRYRGAAGSNAAIEVEGTSLGDPWKETVALRQPAQASTWLAASWARARLRDLEDRYAA
                     GARELEPEIVRVSKQFSVLSRFTAFVAVDRSAAVNPGGVLRQVVQPVEPPTGWGGPRG
                     GGPLAASAMPPPTGYGGVRGLSASGEIQAPERRMRVASASEVTLSEDLATTEMREEPW
                     FDSYGKPPADPYAVPPPMPRPVPGQVQRPSRPVPGMRPLHSPQPAVPVSEAAGVVDTS
                     AAVRAGYVPRLAALAIDLEAQVAGGCDAAAIRLVRQRLTQWIEDLRSV"
BASE COUNT          319 a          854 c         1103 g          361 t
ORIGIN      
        1 gggtgcgcgg gtggtgtcgc tcggcgagcg caccgcgcgg gccggagccg cgccggcagc
       61 gccgtcgtcc gcgtccggag ccgtcgagct gcgcatcgag ctcgcgcccc aggtcgtcct
      121 gtcgctgtcg gtcatcgacg acagcgttgc cgtatcccct gccgacgtgc gcgcgatccg
      181 tgcggccgcg gcgccacttc tggccgagct ggcatcgcgc cggctggcgt cacatcctgg
      241 aggagacgaa tgattgcacc gccgatggtc ttgatgagtg atgaggaggt cgccagatac
      301 ggccgcaacc tggatgcggg gttcggcgtg ctgcaaaccg cgcgggggac gctgccgctg
      361 gtcgcgatgg acgtcgacgc gcgggtcgcc ggggtgatcg cgacgatcga gctggcgcag
      421 cggttcgtca acacgaccgg cggggcgatc gaggcgacgt acatcttccc gctgccggat
      481 cgcgcggcgg tgcatcgctt ccggatggag gtcgccgggc gggtgatcga cggggtgatc
      541 gacgagcgcg gcgcggcgcg tgcgcagtac gacctggcga tcgcggcggg gcatcgcgcg
      601 gcgatcaccg aggaggagcg gccgggggtg ttcacgctgc gggtcggcaa cctgatgccc
      661 ggcgaggcgg cgacggtgcg gctgtcgctg gtcggcccgc tgccggtcga cgacggcgag
      721 gtgacgttcc ggttcccgct ggtggtggcg ccgcgcttca tcccgggggc cgagctcggc
      781 ggcgaccagg ccgggctggg cagcgcggcg gacaccgatc tggtgccgga tgcgtcgcgg
      841 atctcgccgc cggtgcggct gccaggctgc ccgaacccgg tgcggctcgg gctgcgggtc
      901 gcgctggagg acggggtcat gcgcgacgtg gcgtcgagcc tgcatgcggt gacgatggcc
      961 cggcgcgatg cgcaggtgat cgaggtccag cccggcgagc ggctcgatcg cgacttcctc
     1021 ctgcgctggc ggttcgggat cgacggggcc gcgctggcga gctcgctggt gtgcgccgat
     1081 gacccgggcg gcgcggcggg gacgttcgcg ctgacgatcg tgccgccgag cacgcgggcg
     1141 gtcggggcga agccgcgcga cgtggtgttc gtgatcgatc gctcggggag catggcgggg
     1201 tggaagatgg tggcggcgcg gcgagcagcg gcgcggatga tcgacacgct gacctcgcgc
     1261 gaccggttct gcgcgctggc gttcgacaac acggtcgatg cgctgccgga gcccggcttg
     1321 atcgacgcga ccgatcgcca gcggttccgc gcggtcgagg cgctcgcccg ggtcgaggcg
     1381 cggggcggga cggagatcgc acagccgctc ggtcgggcgc tcgagatgct cggcggtggc
     1441 accgacgatc gcgagcgcgt gatcgtgctg gtcaccgacg ggcaggtcgg caacgaggac
     1501 gacgtgctgc gcacggtcgc gcccagcctg cgcaacatca aggtgttcac gctcggcatc
     1561 gaccaggcgg tcaacgcggc gttcctgcgc cggctcgcgg cggcgggggg cgggctgtgc
     1621 gagctggtcg agtccgagga ccggctcgac gcggtgatgg ccaaggtcca ccgccggatc
     1681 ggcacgccga tcgcgaccga gctggcgctg cgcgcgaccg ggctggagct ggagcgcggc
     1741 acgctcgcac cgggcaagct gccggacgtg tacgccgggg cgccggtggt gatcttcggg
     1801 cgctaccgcg gcgcggcggg ctcgaatgcg gcgatcgagg tcgagggcac gagcctggga
     1861 gatccgtgga aggagacggt ggcgctgcgc cagccggcgc aggcgtcgac ctggctggcg
     1921 gcgagctggg cgcgcgcgcg cctgcgcgac ctcgaggacc gctacgcggc gggcgcgcgg
     1981 gagctggagc cggagatcgt gcgggtgtcg aagcagttca gcgtgctgtc gcggttcacc
     2041 gcgttcgtcg cggtcgatcg cagcgccgcg gtgaacccgg gcggtgtgct gcgccaggtg
     2101 gtgcagccgg tcgagccgcc gacgggctgg ggcggtccgc ggggtggtgg accgctcgcg
     2161 gcaagcgcga tgccgccgcc gaccgggtac gggggcgtgc gaggtctctc cgcgtccggg
     2221 gagatccagg cccccgagcg gaggatgcgg gtcgcctccg cgtccgaggt caccctgtcc
     2281 gaggacctcg ccacgacaga aatgcgagag gagccatggt ttgactcgta cggaaagccg
     2341 cccgcggatc cgtacgcggt cccgccgccc atgccgcgac cggtgccggg gcaggtgcag
     2401 cgaccgtcca ggcctgttcc ggggatgcgg ccgctgcaca gtccgcaacc ggcggtgccc
     2461 gtgtccgagg ccgcgggggt cgtggacacg tcggcggcgg tgagagcggg ctacgtcccg
     2521 cggctcgcgg cgctcgcgat cgacctcgag gcgcaggtgg cgggcggttg cgacgcggcg
     2581 gcgatccggc tggtgcggca gcggctgacc cagtggatcg aggacctgcg ctcggtg
//
LOCUS       DASVOR010000163         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_381370,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000163 DASVOR010000000
VERSION     DASVOR010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381370"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VF516_03670"
     CDS             <1..1271
                     /locus_tag="VF516_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106797.1"
                     /translation="REVVHLLGGTVNATSTVGRGSKFIVALPGAIDVAATPRGVSAPG
                     LPLPPGDGPFSVLIIDDDPMVQHLVRGQLEAEQFRVVSAADGVEGLTLAREVRPTVII
                     LDIHLPRLDGWTVLAELKSDLALSQIPVIMMSVEEQRARGFSFGACEYLVKPVEPDRL
                     VHVVRRAIVPEAGDVLIVDDDPQTREVVSRTLRRAGYSTVEARDGEEALLRARVLAPG
                     LMILDLLMPGVDGFEVLRTIRSEGMQMPVVVLTGKKLMPGEERTLRDGLARIVHKGGS
                     ALDSVVREAKQLLVARRVVERGRLPRILYVEDSPQNRDIVRRYLASDYEVIEAEDGEH
                     GLERAVRDTPDLILMDLSLPRLDGWEATRRIKANPAIKHIPVIALTAHAGREDQTRAT
                     EAGCVDYLTKPLERDLLLGAIRKHLPGGRS"
     gene            1268..1636
                     /locus_tag="VF516_03675"
     CDS             1268..1636
                     /locus_tag="VF516_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106798.1"
                     /translation="MTAALERVLVVDDDPMQVELVERGLSRDGFEVRGVTTIADMVVE
                     AQRFAPRIMLIDVNMPDAPGDRAVAVARGAAPGVRIVLYSSWEESRLRVLASQLGADG
                     FISKSDSVTAFGRRLRAMFA"
     gene            complement(1741..2109)
                     /locus_tag="VF516_03680"
     CDS             complement(1741..2109)
                     /locus_tag="VF516_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106799.1"
                     /translation="MVTSGKRILLVDDSPVIRASAAQALDGAGFDVTVRAAFDELLEH
                     GIDGFDLILMDVQMPELYGDDVAFTLRGQRGVTTPIYLFSSIDESELAGLAADAGVDG
                     YIPKSAGLDELVARVRQILG"
     gene            complement(2103..2495)
                     /locus_tag="VF516_03685"
     CDS             complement(2103..2495)
                     /locus_tag="VF516_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106800.1"
                     /translation="MEVTADSRRLLLIDDSEIALAYAESVLTDAGFEVRAVGSLRAFQ
                     TVLSSWRPHLIVTDLYMPEMNGADLCQWLRRQVATARIPIVLCSSAPDHELASVARAV
                     GADAYVSKVDGLDTLPERLNALCNEIVW"
BASE COUNT          421 a          987 c         1056 g          426 t
ORIGIN      
        1 tccgggaggt cgtccacctg ctcggcggca cggtcaacgc gaccagcacg gtcggccgcg
       61 gctcgaagtt catcgtggcg ctgcccggcg cgatcgacgt cgcggcgacg ccgcgcggcg
      121 tcagcgcgcc ggggctgccg ctgccgcccg gcgacgggcc gttctcggtg ctgatcatcg
      181 acgacgatcc gatggtgcag cacctggtgc gcggccagct cgaggccgag cagttccgcg
      241 tggtgtcggc ggccgacggg gtcgagggcc tgaccctggc gcgcgaggtc cggcccaccg
      301 tgatcatcct cgacatccac ctgccgcggc tcgacggctg gaccgtgctc gccgagctca
      361 agagcgatct ggcgctgtcg cagatcccgg tgatcatgat gtcggtcgag gagcagcgcg
      421 cgcgggggtt ctcgttcggc gcgtgcgagt acctggtcaa gccggtcgag cccgaccggc
      481 tggtccacgt ggtccgccgc gcgatcgtgc cggaggcggg cgacgtgctg atcgtcgacg
      541 acgacccgca gacccgcgag gtcgtgtcac gcacgctgcg ccgcgccggg tactcgacgg
      601 tcgaggcccg cgacggcgag gaggcgctgc tgcgcgcccg cgtgctcgcg cccggcctga
      661 tgatcctcga cctgctgatg cccggggtcg acggcttcga ggtgctgcgc acgatccgca
      721 gcgagggcat gcagatgccg gtcgtggtgc tgaccggcaa gaagctgatg cccggcgagg
      781 agcgcacgct gcgcgacggc ctggcccgga tcgtccacaa gggcggcagc gcgctcgaca
      841 gcgtggtgcg cgaggccaag cagctgctgg tcgcccgccg cgtggtcgag cgcggccggc
      901 tgccccggat cctctacgtc gaggactcgc cgcagaaccg cgacatcgtg cggcgctacc
      961 tcgccagcga ctacgaggtg atcgaggccg aggacggcga gcacggcctc gagcgcgcgg
     1021 tgcgcgacac cccggacctg atcctgatgg acctgtcgct gccccggctc gacggctggg
     1081 aggcgacccg ccggatcaag gccaaccccg cgatcaagca catcccggtg atcgcgctga
     1141 ccgctcacgc cgggcgcgag gaccagaccc gggcgaccga ggccggctgc gtcgactacc
     1201 tcaccaagcc gctcgagcgc gacctgttgc tcggcgcgat caggaagcac ctgccgggag
     1261 gccggtcgtg accgccgcgc tcgaacgggt gctggtggtc gatgacgatc cgatgcaggt
     1321 cgagctggtc gagcgcgggc tgtcgcgcga tggcttcgag gtccgcggcg tgaccacgat
     1381 cgccgacatg gtggtcgagg cccagcggtt cgccccgcgg atcatgctga tcgacgtcaa
     1441 catgccggac gcgccgggcg atcgcgcggt cgcggtcgcg cgcggcgcgg cgcccggggt
     1501 gcggatcgtg ctgtactcgt cgtgggagga gtcccggctg cgcgtgctcg cgagccagct
     1561 cggcgccgac ggcttcatct cgaagagcga ctcggtgacc gcgttcggcc ggcggctgcg
     1621 cgcgatgttc gcttaactca agaggcgtgg atggagaggg tagggggggg agaggtcgac
     1681 gtccagggta gagtcaaggt ccaggtccac gtccaggttc cgttcatcga cgcgtttcat
     1741 tcagcccagg atctggcgga cgcgggcgac cagctcgtcg aggccggcgc tcttggggat
     1801 gtagccgtcg acgccggcgt cggcggccag gccggcgagc tcgctctcgt cgatgctgga
     1861 gaacaggtag atcggcgtgg tgacgccgcg ctggccgcgc agggtgaacg cgacgtcgtc
     1921 gccgtacagc tcgggcatct ggacgtccat caggatcagg tcgaagccat cgatgccgtg
     1981 ctcgagcagc tcgtcgaacg cggcgcgcac cgtgacgtcg aagccggcgc cgtcgagcgc
     2041 ctgcgccgcc gatgcccgga tcaccgggct gtcgtcgacc agcaggatgc gcttgccgga
     2101 ggtcaccata cgatctcgtt gcacagcgcg ttgagccgct cgggcagcgt gtcgagccca
     2161 tcgaccttgg agacgtaggc gtcggcgccg accgcgcgcg cgacgctcgc gagctcgtga
     2221 tcgggcgcgc tcgagcacag cacgatcggg atccgcgcgg tcgcgacctg gcggcgcagc
     2281 cactggcaga ggtcggcgcc gttcatctcc ggcatgtaca gatcggtgac gatcaggtgg
     2341 ggcctccagc tcgagagcac ggtctggaac gctcgcaacg accctaccgc ccggacctcg
     2401 aatccggcat ccgtgagcac ggactccgcg taggccagcg cgatctcgct gtcatcgatc
     2461 agcagcaacc ggcggctgtc ggcggtgacc tccacgctcg acgcatggta gcgcgatcat
     2521 cggcgcagga tcgcgattcg ttccgtctat cacgctcctt gccatgccca tggtcccgcc
     2581 gaggtccact cctggtcccg ccgaggtcca cgcccctggc cacgcccctg gcagcggcct
     2641 gtccgcggcc gcgacttgac ctcgcagggc gcactcgatc gtacgcggtg tggtatggac
     2701 cggccatgtc ggagtccaag cgcaatgacc agaagtgggc cgcacaggcg atgtcgaaga
     2761 tcgtgtccgc gctgaatcgc gccgacgaga tcggcggcga gctgcgcgac taccttcagg
     2821 acaagatggc ccatgatccg cgggttgcgc aggcgcgcag tcagatcgcg cgacttctcg
     2881 gccgccggga
//
LOCUS       DASVOR010000164         3556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_1598,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000164 DASVOR010000000
VERSION     DASVOR010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3556
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1598"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..829
                     /locus_tag="VF516_03690"
     CDS             14..829
                     /locus_tag="VF516_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8106801.1"
                     /translation="MLERPAPRAAPVTAPRAGLIAGIGASAVAIAQEVGGIVLLTAEV
                     VRALVPPQIDRRELWKSLYKMGNRSVPIVVLTALFAGGLMSLQAAPFVKKFGATALAG
                     WAAGYAVLRELGPILIALMFSGRVGANNTAELSTMTVTEQLDGLRALAIDPVQFLVVP
                     RVLAMMTTLVALTVIGDLVALTGAAIIGRVMLDIEWSTMYHGFVDNLRPFDFLHGIYK
                     AIAFGAAIAVSSCYFGVTVRGGAVGVGRAVNNAVVAAAVSIMLLDFFLTYLTG"
     gene            826..1704
                     /locus_tag="VF516_03695"
     CDS             826..1704
                     /locus_tag="VF516_03695"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8106802.1"
                     /translation="MMDADPRSIPHRAAGGVQRPIAAVGAALLATGAAARELWLVYLA
                     TWAGVARRVSRRHLQHGPHGRQGVSHGEIVRQLYWIGNRSIVFIAVTLGFIGMVMAYE
                     ACLQLSRVTGDFSQVGGQLLKLDVSDFAATLTGLMLATRVGAGIAAEIGSMKVTEQID
                     ALRMSGVLPIDYLIVPRFIASLVMTLALSVLGGVVMYAAGGLTAKYSFAVNPGVFFDA
                     SMVRGRHVALLVVKAEAYGAAIPVVAGFCGLRARGSSEGVGWATTAAVIGGSFAVIVL
                     DFAISAAALVMFGGDL"
     gene            1701..2471
                     /locus_tag="VF516_03700"
     CDS             1701..2471
                     /locus_tag="VF516_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8106803.1"
                     /translation="MTEPAAFIEYRAVAKAFGGQPVLRDMSLAIRRGEVMYIIGTSGV
                     GKSVTIKLLIGLLRVDRGEIWFDGRRIDQLAERALVPLRKRIGMVFQSSTLFDSMTLA
                     ENVALPLRKHRRLAWPAAIDEARRRLAQVYMAEHAERYPAELSDGMRKRTAIARTLAL
                     DPEVVLFDEPTTGLDPVSARRIDRLIRELASSLGVTAVVVSHDPPSIFGTADRVALLY
                     QGAVHALATPAELRASPDPIVQQFITGQSSGPMETPGF"
     gene            2558..3115
                     /locus_tag="VF516_03705"
     CDS             2558..3115
                     /locus_tag="VF516_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106804.1"
                     /translation="MSTLSTAAWAAHDVGLATAIGGTLYGRAALEPALREIADPAERD
                     RVATSAWQRFTWLNLAAHGVFAATWFIGRSMLSGREVSCTARRMTQVKDGLVIASLVT
                     GIGTAVLGRMLGKCARRGEGPAQVREAGGTARGGERTVALQRAVGGLGIANLVANIAT
                     LGITAALAMEGSRSPRFSARSRFLP"
     gene            complement(3248..>3556)
                     /locus_tag="VF516_03710"
     CDS             complement(3248..>3556)
                     /locus_tag="VF516_03710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106805.1"
                     /translation="RAAIDLLAPRLAQTTAELARIRGHLVAGDPAGRAEQILATLRRA
                     LDQVDPLLAAAGELAQRIARGEGSLGRLLMDPEFPEDTKELGKIMKRQPWRIIQKPPN
                     "
BASE COUNT          435 a         1266 c         1310 g          545 t
ORIGIN      
        1 ggtatcgtag tgcgtgctcg agcgacccgc gccgcgggca gccccggtga ccgcgccgcg
       61 cgccgggctc atcgccggga tcggcgcgag cgcggtggcc atcgcccagg aggtcggcgg
      121 gatcgtcctg ctcaccgccg aggtcgtgcg cgcgctggtg ccgccgcaga tcgaccggcg
      181 cgagctgtgg aagagcctct acaagatggg caaccgctcg gtgcccatcg tcgtgctcac
      241 cgcgctgttc gcgggcggcc tgatgtcgct gcaggcggcg ccattcgtga aaaagttcgg
      301 cgccactgca ctggccggct gggcggccgg ctacgcggtg ctgcgcgagc tcggcccgat
      361 cctgatcgcg ctgatgttct ccggccgggt cggggccaac aacaccgccg agctgtcgac
      421 gatgacggtg accgagcagc tcgacgggct gcgcgcgctg gcgatcgatc cggtgcagtt
      481 cctcgtggtg ccgcgcgtgc tggcgatgat gacgacgctg gtcgcgctga ccgtgatcgg
      541 cgatctcgtc gcgctgaccg gcgccgcgat catcggccgg gtgatgctcg acatcgagtg
      601 gtcgacgatg taccacggct tcgtcgacaa cctgcggccg ttcgacttcc tgcacggcat
      661 ctacaaggcg atcgcgttcg gcgccgcgat cgcggtgtcg tcgtgctact tcggcgtcac
      721 cgtgcgcggc ggcgcggtcg gcgtcggtcg cgcggtcaac aacgcggtgg tcgccgcggc
      781 ggtgtcgatc atgctgctcg acttcttcct gacctacctg acgggatgat ggacgccgac
      841 ccgcgctcga ttccccaccg cgccgcgggc ggcgtccagc ggccgatcgc cgccgtcggc
      901 gccgcgctgc tcgccaccgg cgcggccgcc cgcgagctct ggctggtcta cctcgcgacc
      961 tgggcgggtg tcgcgcgccg ggtgtcgcgc cgccacctcc agcacggccc gcacggccgg
     1021 cagggcgtct cgcacggcga gatcgtccgc cagctgtact ggatcggcaa ccgctcgatc
     1081 gtgttcatcg cggtgaccct cggcttcatc ggcatggtga tggcctacga ggcctgcctg
     1141 cagctgtcgc gggtcaccgg cgacttctcc caggtcggcg gccagctgct caagctggac
     1201 gtgtccgact tcgcggcgac cttgaccggg ctgatgctcg ccacccgggt cggcgccggc
     1261 atcgccgccg agatcggctc gatgaaggtg accgagcaga tcgacgcgct gcggatgagc
     1321 ggcgtgctgc cgatcgacta cctgatcgtg ccgcggttca tcgcctcgct ggtcatgacc
     1381 ctcgcgctca gcgtgctcgg cggcgtcgtg atgtacgccg cgggcgggct gaccgcgaag
     1441 tactcgttcg cggtcaaccc gggcgtgttc ttcgacgcgt cgatggtgcg cggccgccac
     1501 gtcgcgctgc tggtcgtcaa ggccgaggcc tacggcgccg cgatcccggt ggtcgcgggg
     1561 ttctgcggcc tgcgcgcccg gggcagctcc gagggcgtcg gctgggcgac caccgccgcg
     1621 gtgatcggcg gctcgttcgc ggtcatcgtg ctcgacttcg cgatctcggc ggccgcgctg
     1681 gtgatgttcg gcggtgacct gtgaccgagc cggccgcgtt catcgagtac cgcgcggtcg
     1741 ccaaggcgtt cggcggccag ccggtgctgc gcgacatgag cctggcgatc cggcgcggcg
     1801 aggtcatgta catcatcgga acgtcgggcg tcggcaagtc ggtgacgatc aagctcctga
     1861 tcggcctgct ccgcgtcgat cgcggcgaga tctggttcga cggccggcgc atcgatcagc
     1921 tcgccgagcg cgcgctggtg ccgctgcgca agcggatcgg catggtgttc cagagctcga
     1981 ccctgttcga ctcgatgacg ctggccgaga acgtcgcgct gccgctgcgc aagcaccggc
     2041 gcctggcgtg gccggccgcg atcgacgagg cgcgccgccg gctcgcccag gtctacatgg
     2101 ccgagcacgc cgagcgctac ccggccgagc tgtccgacgg catgcgcaag cgcaccgcga
     2161 tcgcgcgcac cctggcgctc gatcccgagg tcgtgctgtt cgacgagccg accaccggcc
     2221 tcgacccggt cagcgcgcgc cggatcgatc gcctgatccg cgagctcgcc tcgagcctcg
     2281 gcgtcaccgc ggtcgtggtg tcccacgatc cgccgtcgat cttcggcacc gccgaccgcg
     2341 tcgccctgct ctaccagggc gccgtgcacg cgctcgccac gccggccgag ctgcgcgcgt
     2401 cgcccgatcc gatcgtccag cagttcatca ccggacagtc gtcggggccg atggagacgc
     2461 cggggttctg accggcgtgg gcgcgctccg cggtgcgcgg gtgcgcacgg ccgatcggga
     2521 ccgcggcgtg catgaccgcg gggcggaggt atgaatgatg agcacactct cgacagcagc
     2581 ctgggccgcg cacgatgtcg gactcgccac cgcgatcggc ggcacactct acggacgtgc
     2641 cgccctggaa ccggcgctcc gcgagatcgc ggatccggcc gagcgcgatc gggtcgcgac
     2701 cagcgcctgg cagcggttca cctggctcaa cctcgccgcg cacggcgtgt tcgccgcgac
     2761 ctggttcatc gggcgctcga tgctgagcgg ccgcgaggtc agctgcacgg cccgcaggat
     2821 gacccaggtc aaggacgggc tcgtgatcgc gagcctggtc accggcatcg ggaccgccgt
     2881 gctgggacgc atgctcggca agtgcgcccg gcgcggcgag ggcccggccc aggtgcgcga
     2941 ggcgggcggc accgcgcgcg gcggggagcg caccgtggcg cttcagcggg cggtaggcgg
     3001 cctcggcatc gccaacctgg tcgccaacat cgcgaccctc ggcatcaccg cggcgctcgc
     3061 gatggagggc agccgatcgc cccggttctc ggcgcgcagc cggttcctgc cgtgacccgc
     3121 cggccgcggg atcctcaccc cgcggccgac ctcgccggcg gcatcgtgcc gagctggcgg
     3181 agcatcgcga ggtcgtcgcg ttagcctcgc ggtggaccga gccgctggag atcaggcgat
     3241 cgcgatctca gttcggcggc ttctggatga tccgccacgg ctggcgcttc atgatcttgc
     3301 ccagctcctt ggtgtcctcg gggaactcgg ggtccatgag caggcggccg agcgagccct
     3361 cgccgcgggc gatccgctgg gcgagctcgc cggcggccgc gagcagcgga tcgacctgat
     3421 cgagcgcgcg gcgcagcgtc gcgaggatct gctcggcgcg gccggccgga tccccggcga
     3481 ccaggtggcc gcggatccgg gcgagctcgg cggtggtctg tgcgagccgg ggagcgagca
     3541 ggtcgatcgc ggcgcg
//
LOCUS       DASVOR010000165         5953 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_30708,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000165 DASVOR010000000
VERSION     DASVOR010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5953)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5953)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5953
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30708"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..1442)
                     /locus_tag="VF516_03715"
     CDS             complement(81..1442)
                     /locus_tag="VF516_03715"
                     /inference="COORDINATES: protein motif:HMM:NF011649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HEX8106806.1"
                     /translation="MQLASTIRGVRYAFGSVKEVLAKANPPKSGDDLAGVSARDSVER
                     VAARAVLADLTLRELREHPVVPYERDELTRHVEDELDAQAFAAVAHLTVGQFREWLLD
                     IATTGPTLRAIAPGLTPEMAAAACKLMSNFDLMTVGAKCQVVVRANNTLGLPGRLSSR
                     CQPNHPTDDVAGILASAREGLAYGCGDAVIGVNPATDTPASTAEILRAVDDLLRRNAI
                     PTQHCVLSHVTVQMKALELGCPMDLMFQSLAGTEAANTAFGISVALLDEAWETIKARG
                     TARGPNRMYFETGQGSALSSAAHHGADQVTIEARAYGLARRYRPFLVNTVVGFIGPEY
                     LEDGPQISRAALEDHFMGKLLGLPMGCDACFTYHARMTQDELESLKVLLGAAGCAYLM
                     ALPVGDDIMLNYQSTSYHDIPTLRRLLGKRPTPEFDAWLEARGIWRGDAPGPRFGDVG
                     SVR"
     gene            complement(1538..2221)
                     /locus_tag="VF516_03720"
     CDS             complement(1538..2221)
                     /locus_tag="VF516_03720"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX8106807.1"
                     /translation="MQTREGMYSKARIAGHPIHPMLVAFPLAFYTATVVTLLVYIGTR
                     EAFWYRFAMVASLAGIATAVIAAIPGAIDLRSLPRRSRARKAGLQHAGFNLLATGLFA
                     VTALVLYQTWIHRTMINGEYIFDATVPLALAVVAWVAMVIAGSLGWTLVQTHHVGVKP
                     ALVHPDRPSREPELEAPEVHAIHPVRDLREIPTAPEVPAAIDDLAARRAHRRATSSLL
                     QRFPDDHPA"
     gene            complement(2314..3438)
                     /locus_tag="VF516_03725"
     CDS             complement(2314..3438)
                     /locus_tag="VF516_03725"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8106808.1"
                     /translation="MTLYDELTAGRARFIRVDELCRRGAELRPDLLPSTAELAAEAAR
                     MQRDKLGVERKQGAFLADVLDDPEAGAHLCHAMLLPHPASAARLAEYAATGVLELPGA
                     SVTRVGKASVVTTRNPRFLNAEDESTLDGFEIAIDVATLDPHSEICVLRGGAVEHPKY
                     AGRRLLGAGINLTHLYQGRIRYLWFLIRDLGLVNKLYRGVALPDVPPEQGGVEKPWVA
                     VVEGFAIGGHCQILLTVDYTIAADDAYLTLPARKEGILPGAANLRLPRFVGDRIARQA
                     IMNERRIDCASPEGQLICDAIVAPGEIDRAIGAIIDRLTNAGVVSAAGNRRAIRIAQE
                     PLDVFRRYMSVYAREQAHCYLSPALVQNLERHWDAGSRRG"
     gene            3587..4807
                     /locus_tag="VF516_03730"
     CDS             3587..4807
                     /locus_tag="VF516_03730"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEX8106809.1"
                     /translation="MAPKIQVKNPIVEMDGDEMTRIIWRFIKDKLITPYLDLDIKYFD
                     LGIENRDRTNDQVTIESANATKQHGVAVKCATITPDEARVKEFGLKEMWKSPNGTIRN
                     IIGGTIFREPIVCRNVPRLIPGWTRPIVIGRHAFGDQYRATDFVVPGPGTLTMTFTPA
                     GGGKPTEMKIFDFPGGGVALGMYNLDESIIGFARSSFNYGLDRGWPVYMSTKNTILKR
                     YDGRFKDLFQKVFDEEFADKFKAKGLVYEHRLIDDMVASALKWEGGFVWACKNYDGDV
                     QSDSIAQGFGSLGLMTSVLMTPDGKTVEAEAAHGTVTRHYREHQKGRPTSTNPIASIY
                     AWTQGLKYRGKFDATPDVVAFADALEKVCVETVEAGKMTKDLALLIDASHPWLTTEQF
                     LDALDAALRAKLAG"
     gene            4824..5783
                     /locus_tag="VF516_03735"
     CDS             4824..5783
                     /locus_tag="VF516_03735"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8106810.1"
                     /translation="MSGITLVTGSAGHLGEALVRTLRAAGRPVRGLDRLASPFTDVVG
                     SITDAAVVERCMDGAGDVIHAATLHKPHVATHSRQQFVDTNITGTLTLLEAAVRARVR
                     AFVFTSTTSAFGDALTPPPGAPAAWITEDVVPVPKNIYGATKVAAEDLCQLAHRNQGL
                     ACLVLRTSRFFPEPDDDPAARDGYADANLKANEFLNRRLDIEDAVDAHLCALERAPGL
                     GFGRYIISATTPLRPEDAALLRRDAPAAVSCRVPSWEAIYARLGWRMAPSIDRVYDNA
                     RARAELGWQPRHDFAAVLARARDTGDVRSPLARAIGARGYHRD"
BASE COUNT          897 a         2137 c         2076 g          843 t
ORIGIN      
        1 gtcgggtccg gctcaccggg atagctcacc acgcgccgcg gcgccggcgc aaccggtcgc
       61 tgcgcggctc accggacggc tcatcgtacg gaccccacgt cgccgaaccg gggccccggg
      121 gcgtcgccgc gccagatgcc ccgggcctcg agccaggcgt cgaactccgg cgtggggcgc
      181 ttgccgagca gccgccgcag ggtcgggatg tcgtggtagc tcgtcgactg gtagttcagc
      241 atgatgtcgt cgccgaccgg gagcgccatc aggtaggcgc agcccgcggc gccgagcagc
      301 accttgaggc tctcgagctc gtcctgggtc atccgcgcgt ggtaggtgaa gcacgcgtcg
      361 cagcccatcg gcaggccgag cagcttgccc atgaagtggt cctcgagcgc cgcgcgcgag
      421 atctgcggac cgtcctcgag gtactcgggc ccgatgaagc cgaccacggt gttgaccagg
      481 aacggccggt agcgccgggc caggccgtac gcgcgggcct cgatcgtcac ctggtccgcg
      541 ccgtggtgcg ccgccgacga cagcgccgag ccctggccgg tctcgaagta catccggttg
      601 gggcccctgg cggtgcccct ggccttgatg gtctcccacg cctcgtcgag cagcgccacg
      661 ctgatcccga acgcggtgtt ggcggcctcg gtcccggcca gcgactggaa catcaggtcc
      721 atcgggcagc cgagctcgag cgccttcatc tgcaccgtga cgtgggacag cacgcagtgc
      781 tgggtcggga tcgcgttgcg gcgcagcagg tcgtcgaccg cgcgcaggat ctcggcggtc
      841 gacgcggggg tgtcggtcgc cgggttgaca ccgatcaccg cgtcgccgca gccgtaggcc
      901 aggccctcgc gcgccgacgc gaggatgccc gcgacgtcgt cggtcgggtg gttgggctgg
      961 cagcgcgacg acagccggcc gggcaggccg agcgtgttgt tggcccgcac caccacctgg
     1021 cacttggcgc cgaccgtcat caggtcgaag ttcgacatca gcttgcaggc cgccgccgcc
     1081 atctcggggg tcagcccggg cgcgatcgcg cgcagcgtcg gcccggtggt ggcgatgtcg
     1141 agcagccact cgcggaactg gccgaccgtg aggtgagcga ccgcggcgaa cgcctgggcg
     1201 tcgagctcgt cctcgacgtg ccgggtcagc tcgtcgcgct cgtacggcac caccgggtgc
     1261 tcgcgcagct cgcgcagcgt gaggtcggcg agcaccgcgc gcgccgcgac ccgctcgacc
     1321 gagtcgcgcg ccgacacccc ggcgaggtcg tcgccgctct tgggcgggtt cgccttggcg
     1381 agcacctcct tgaccgagcc gaacgcgtag cgcacgccgc ggatcgtcga tgccagctgc
     1441 acgggcccgg agcatacaaa gaaacaccgc ccgtggcctc ggatccaggg accctcggtc
     1501 acgggcggtg ggatgtaagc caactaggcg gtaccagcta ggcggggtga tcgtcaggga
     1561 aacgctggag caaggaagaa gtagcgcgcc ggtgcgcgcg gcgggcggcc aggtcgtcga
     1621 tcgccgcggg gacctcgggc gccgtgggaa tctcgcggag gtcgcggacg gggtggatgg
     1681 cgtgaacctc cggcgcctcg agctcgggct cgcgggacgg ccggtcgggg tggaccagcg
     1741 caggcttgac gccgacgtgg tgggtctgga ccagcgtcca gccgagcgag ccggcgatca
     1801 ccatcgccac ccacgcgacc acggccagcg cgagcggcac cgtcgcgtcg aagatgtact
     1861 cgccgttgat catcgtgcga tggatccagg tctggtacag caccagcgcg gtcaccgcga
     1921 acagcccggt ggccagcagg ttgaagccgg cgtgctggag gccggccttg cgggcgcgcg
     1981 agcggcgcgg cagcgaccgc agatcgatcg cccccgggat cgcggcgatc accgcggtgg
     2041 cgatcccggc gaggctcgcc accatcgcga agcggtacca gaacgcttcg cgcgtcccga
     2101 tgtacaccag cagcgtcacc accgtggcgg tgtagaacgc gagcggaaac gccacgagca
     2161 tcggatgaat cgggtgaccc gcgatgcgtg ccttgctgta catgccttcc cgcgtctgca
     2221 tcgtgtgtgc cggtgcaatc ccccgtttcc gctcgacggg gcgcggcggc tgtgtgcgcg
     2281 gttcgtgcgc gcgagcgcga gcgcaccaca ccgtcagccg cggcggctac ctgcgtccca
     2341 gtggcgctcc aggttctgta cgagcgccgg actgagatag cagtgggcct gctcgcgcgc
     2401 gtagacggac atgtagcggc ggaacacgtc gagcggttcc tgcgcgatcc ggatcgcgcg
     2461 gcggttgccc gcggcgctca ccacgccggc gttggtcagc cgatcgatga tcgcaccgat
     2521 cgctcggtcg atctctcccg gcgcgacgat cgcgtcacag atcagctggc cctcggggct
     2581 cgcgcagtcg atccggcgct cgttcatgat cgcctggcgc gcgatgcggt cgccgacgaa
     2641 gcgcggcagc cgcaggttgg cggcgccggg caggatgccc tccttgcgcg ccggcagggt
     2701 gaggtacgcg tcatcggcgg cgatcgtgta gtccacggtg agcaggatct ggcagtgccc
     2761 gccgatcgcg aagccctcga ccaccgcgac ccacggcttc tcgacgccgc cctgctcggg
     2821 cgggacgtcg ggcagcgcca cgccgcgata cagcttgttg accagcccga ggtcgcggat
     2881 caggaaccac aggtacctga tcctgccttg atacaggtgg gtgagattga tgccggcgcc
     2941 gagcagccgc ctgccggcat acttggggtg ctcgaccgcg ccgccgcgca gcacgcagat
     3001 ctcgctgtgc ggatcgagcg tcgccacgtc gatcgcgatc tcgaagccgt cgagcgtgga
     3061 ctcatcctcg gcgttgagga atcgcgggtt gcgcgtggtg accaccgacg ccttgccgac
     3121 ccgcgtcacg ctcgcgccgg gcagctccag cacgccggtc gccgcgtact cggcgagccg
     3181 cgcggccgac gccgggtgcg gcagcagcat cgcgtgacac aggtgcgcac cggcctcggg
     3241 gtcgtccagc acgtcggcga ggaatgcgcc ttgcttccgc tcgacgccga gcttgtcgcg
     3301 ctgcatgcgg gcggcctccg ccgcgagctc ggcggtcgag ggcaggaggt cggggcgcag
     3361 ctccgccccc cggcggcaca gctcgtcgac ccggatgaac cgtgcacggc cagcggtcag
     3421 ctcgtcgtaa agcgtcatgc gcggagtgta cattcgcgga tttccgcgtc gcggctgtgg
     3481 cgcggctcgc gatgcatgac gctccccgcc atgcggcgcg ttcaccccgg gcgggtccgc
     3541 ccgtgcgtct gccgcagatc gagacaaggt gatacaacac gccgccatgg ccccgaagat
     3601 ccaggtcaag aaccccatcg tcgagatgga cggcgacgag atgacgcgca tcatctggcg
     3661 cttcatcaag gacaagttga tcaccccgta cctcgacctc gacatcaagt acttcgacct
     3721 cgggatcgag aaccgcgatc gaaccaacga tcaggtcacc atcgagtcgg cgaacgcgac
     3781 caagcagcac ggcgtcgcgg tcaagtgcgc gaccatcacg ccggacgagg cgcgggtcaa
     3841 ggagttcggg ctcaaggaga tgtggaagtc gcccaacggc acgatccgca acatcatcgg
     3901 cggaacgatc ttccgcgagc cgatcgtgtg ccgcaacgtg ccgcgcctga tcccgggctg
     3961 gacccggccg atcgtgatcg gccgccacgc gttcggcgat cagtaccgcg cgaccgactt
     4021 cgtggtcccc ggcccgggca cgctgaccat gacgttcacg cccgccggcg gcggcaagcc
     4081 gaccgagatg aagatcttcg acttcccggg cggcggcgtc gcgctcggca tgtacaacct
     4141 cgacgagtcg atcatcgggt tcgcgcggtc gagcttcaac tacggcctcg accgcggctg
     4201 gccggtgtac atgtcgacca agaacacgat cctcaagcgc tacgacggcc ggttcaagga
     4261 cctgttccag aaggtgttcg acgaggagtt cgccgacaag ttcaaggcca agggcctggt
     4321 ctacgagcac cgcctgatcg acgacatggt cgcgtccgcg ctcaagtggg agggcggctt
     4381 cgtgtgggcg tgcaagaact acgacggcga cgtccagtcg gactcgatcg cccagggctt
     4441 cggctcgctc gggctcatga cctcggtgct gatgactccc gatggcaaga ccgtcgaggc
     4501 cgaggcggcg cacggcacgg tcacccgcca ctaccgcgag caccagaagg gccggccgac
     4561 ctcgaccaac ccgatcgcct cgatctacgc ctggacccag ggcctcaagt accgcggcaa
     4621 gttcgacgcc acgcccgacg tcgtcgcgtt cgccgacgcg ctcgagaagg tctgcgtcga
     4681 gaccgtcgag gccggcaaga tgaccaagga cctcgcgctg ctgatcgatg ccagccaccc
     4741 gtggctgacc accgagcagt tcctcgacgc gctcgacgcc gccctgcgcg ccaagctcgc
     4801 cggctgaccg gccggctcgc gcgatgtccg gcatcacgct ggtcaccggc tcggccggcc
     4861 acctcggcga ggccctggtc cgcacgctgc gcgccgccgg tcggccggtc cgcggcctcg
     4921 atcgcctggc gtcgccgttc accgacgtcg tcggatcgat caccgacgcc gcggtcgtcg
     4981 agcgctgcat ggacggggca ggggacgtga tccacgccgc gacgctgcac aagccgcacg
     5041 tcgcgaccca cagccgccag cagttcgtcg acaccaacat caccggcacg ctgaccctgc
     5101 tcgaggcggc ggtccgcgcc cgggtccgcg cgttcgtgtt caccagcacc accagcgcgt
     5161 tcggcgacgc gctgaccccg ccgccgggcg cgccggccgc gtggatcacc gaggacgtcg
     5221 tcccggtgcc caagaacatc tacggcgcca ccaaggtcgc cgccgaggat ctctgccagc
     5281 tcgcgcaccg caaccagggc ctggcctgcc tggtgctccg cacctcgcgg ttcttccccg
     5341 agcccgacga cgatcccgcg gcgcgcgacg gctatgccga cgccaacctc aaggccaacg
     5401 agttcctgaa ccgccggctc gacatcgagg acgcggtcga cgcccacctg tgtgcgctcg
     5461 agcgcgcccc gggcctgggc ttcggccgct acatcatctc cgccacgacc ccgctgcgcc
     5521 ccgaggacgc cgcgctactg cgccgcgacg cgcccgcggc cgtgagctgc cgcgtcccca
     5581 gctgggaggc gatctacgcc cggctcggct ggcggatggc gccgtcgatc gatcgggtct
     5641 acgacaacgc gcgcgcccgg gccgagctcg gctggcagcc gcgccacgac ttcgcggcgg
     5701 tgctcgcccg cgcccgcgac accggcgacg ttcgcagccc gctggcgcgc gcgatcggtg
     5761 ccaggggcta ccaccgcgac tagctcagga agccgcgcgc gatgttcatc agctcggtgt
     5821 tggcggcgac cgccttggtg aacgcttcct tctcggcagc cgacagctcg atctcgtaga
     5881 tccgctcggc gccgcgcgcc gacagcttga ccgggacgcc ggcgaacgcg tccttgaccc
     5941 cgaactcgcc gtg
//
LOCUS       DASVOR010000166         2931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_147722,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000166 DASVOR010000000
VERSION     DASVOR010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147722"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..1776)
                     /locus_tag="VF516_03740"
     CDS             complement(151..1776)
                     /locus_tag="VF516_03740"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8106811.1"
                     /translation="MKSVSAGVRVAVLFLLLAIAGYLVWKNLGQNPAGKDNYTLFAKF
                     RDASGLPKGTKVVIAGLPKGEVVGLEIEGRYAKVTFKLDNEITVWTSAVIMKKARSLL
                     GDNYLEIDPGEPVKQAPDGTRQTFTQLGPPCPAYRDPDPRKSEACRQIVNVIEATTPD
                     QLLHRIEQTLPNVDRVLESVRDLSEDVRRIVNGPLQSVATRVDGLVQREAPAVSDIIE
                     RADRSMARIEQITADLHSITKGADPKIARILANLDDASAEAKDLVSTARSELTQTGNA
                     IRGKLDKLDGVITSTESITRKIDEDKGTLGRLVNDPEIADNVEEITDQAKGFLGTLFG
                     LKTYVGLRSEYNVGAGLLRHYISVELHTRPDKFYLVELEKGPRGAYPDVTLTFDPTVD
                     PNHWIRRTVIQDSIRFTFQFAKRFSWLTLRYGLKESTGGVGADADLQWFDRDLRLSVD
                     VFDASWDQYPRVKLTAAYEVFRHVYVLGGVDELLNTPRNLPIVTGDTPVPIQFQTFRY
                     GRDFFFGGMLRFNDEDLSALLAVGGSALGAAAR"
     gene            complement(1900..>2931)
                     /locus_tag="VF516_03745"
     CDS             complement(1900..>2931)
                     /locus_tag="VF516_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8106812.1"
                     /translation="DEQTRAAHLCRRAAVSVPRDPDSEPTNVRDPDGRVPGRVLAGQE
                     AAGDTDMPAPESPPPSNGEAAAGPAGRDGKTVGGAVLEGIKEAGAPVMRLLENLGGHL
                     ILVGRALSWLPRRPFRWANYLEAAEYIGFGSLPIVVLVGFFTGAVTSLQSVAAFRQFG
                     LESFSGGTTGKALSTELGPVLTSLMLAGRAGAGIATELGTMRITEQIDALESMAVNPI
                     QYLVLPRLIAGAIVTPILSLLFFVVGMGGAWLVAVVLQGVDQGQWVANLRDIVDLIDV
                     AQGLIKSVFFGFMVALVGCYQGFNATGGGRGVGMGTTRAVVIASVVTLVGDYFQTDIL
                     LAIFRTGKT"
BASE COUNT          435 a         1072 c          974 g          450 t
ORIGIN      
        1 cccctcccgc caccggcgcc gcccgcgagc gcatcgtgcg cgaggccgcc gcggtcgacg
       61 acgccgagct ccgcgagctg atcgcccggg tccgcatcac ccacgatcgc tgacttcgat
      121 acgcgcttcg cgatccgctt ccatacgtgc tcagcgcgcc gccgcgccca gcgccgagcc
      181 gccgaccgcc agcagcgccg acaggtcctc gtcgttgaac cgcagcatgc cgccgaagaa
      241 gaagtcgcgg ccgtagcgaa acgtctggaa ctggatcggc accggcgtgt cgcccgtgac
      301 gatcggcaag ttccgcggcg tgttgagcag ctcgtcgacc ccgcccagca cgtagacgtg
      361 gcgaaacacc tcgtaggcgg cggtgagctt gacccgcggg tactggtccc acgaggcgtc
      421 gaacacgtcc accgacagcc ggagatcgcg atcgaaccac tgcaggtcgg cgtcggcgcc
      481 gaccccgccg gtcgactcct tgagcccgta gcgcagggtc agccacgaga accgcttcgc
      541 gaactggaac gtgaaccgga tcgagtcctg gatcaccgtg cggcggatcc agtggttcgg
      601 gtcgaccgtc ggatcgaacg tcagcgtgac gtcggggtac gcgccgcgcg gccccttctc
      661 gagctcgacc aggtagaact tgtccggccg ggtgtggagc tcgacgctga tgtagtggcg
      721 caacaggccg gccccgacgt tgtactcgct gcgcagcccg acgtacgtct tgagcccgaa
      781 cagcgtgccg aggaagccct tggcctggtc ggtgatctcc tcgacgttgt cggcgatctc
      841 cggatcgttg accagccggc cgagcgtgcc cttgtcctcg tcgatcttgc gggtgatcga
      901 ctcggtgctg gtgatcacgc cgtcgagctt gtcgagcttg ccgcggatcg cgttgccggt
      961 ctgggtcagc tcgctcctgg cggtcgacac caggtccttg gcctcggccg acgcgtcgtc
     1021 gaggttggcg aggatgcggg cgatcttggg gtcggcgccc ttggtgatcg agtgcaggtc
     1081 ggccgtgatc tgctcgatcc gcgccatcga gcggtcggcg cgctcgatga tgtcgctgac
     1141 cgcaggggcc tcgcgctgga ccagcccgtc gacccgggtc gcgaccgact gcagcggccc
     1201 gttgacgatc cggcgcacgt cctcggacag atcgcgcacg ctctccagca cgcgatcgac
     1261 gttgggcagc gtctgctcga tccggtgcag cagctggtcc ggcgtggtcg cctcgatcac
     1321 gttgacgatc tgccggcagg cctccgactt gcggggatcc gggtcgcggt acgccgggca
     1381 cggcgggccg agctgcgtga atgtctgacg cgtgccgtcc ggcgcctgct tgaccggctc
     1441 gcccggatcg atctccaggt agttgtcgcc gagcagcgag cgcgccttct tcatgatcac
     1501 cgcgctggtc cacaccgtga tctcgttgtc gagcttgaac gtgaccttgg cgtagcggcc
     1561 ctcgatctcc agcccgacga cctcgccctt gggcaggccc gcgatcacga ccttggtgcc
     1621 cttgggcagc ccggacgcat cccggaactt cgcaaacagc gtgtagttgt ccttgcccgc
     1681 cgggttctgc ccgaggttct tccagaccag gtagcccgcg atcgccagga gcaggaacag
     1741 gaccgcgacg cggacccctg cggagaccga cttcatgcgc cgcctccggc ggagaccgcg
     1801 cgcgcaccgg cggcgctgca gctcacggac tggtcgcgag tcaccacctc ggcgaaggta
     1861 taccgcgatc cgctgcgatg gggcgcgccg tgcgccgtat cacgtcttgc cggtccgaaa
     1921 gatcgccagc aggatgtcgg tctggaagta gtccccgacc agggtcacca ccgaggcgat
     1981 caccaccgcg cgcgtcgtcc ccatcccgac cccgcggcca ccgccggtcg cgttgaaccc
     2041 ctggtagcag ccgaccagcg ccaccatgaa gccgaagaac acgctcttga tcagcccctg
     2101 ggccacatcg atcaggtcga cgatgtcgcg caggttggcg acccactggc cctggtcgac
     2161 gccctggagc accaccgcga ccagccacgc gccgcccatg ccgaccacga agaacaggag
     2221 cgacaggatc ggcgtgacga tcgccccggc gatcagccgc ggcagcacga ggtactggat
     2281 cggattgacc gccatcgact ccagcgcgtc gatctgctcg gtgatccgca tcgtgccgag
     2341 ctcggtggcg atcccggccc cggcccggcc ggcgagcatc agcgacgtca gcaccggccc
     2401 gagctcggtc gacagcgcct tgccggtcgt gccgcccgag aacgactcca ggccgaactg
     2461 gcggaacgcc gccaccgact ggagcgacgt caccgcgccg gtgaagaacc cgaccagcac
     2521 gacgatcggc aggctgccga acccgatgta ctcggcggcc tcgaggtagt tcgcccagcg
     2581 gaacggccgg cgcggcagcc acgacagcgc gcggccgacc aggatcaggt gcccgccgag
     2641 gttctccagc aggcgcatca ccggcgcccc ggcttccttg atgccctcca gcaccgcgcc
     2701 gcccaccgtc tttccgtcgc ggcccgccgg cccggccgcg gcctcgccgt tgcttggcgg
     2761 tgggctctcc ggcgccggca tatccgtgtc cccggccgcc tcctggccgg cgagcacccg
     2821 ccccgggacc cgaccgtcgg gatcgcggac gttcgtcggc tcgctgtcgg gatcgcgcgg
     2881 cacgctcacg gcggctctcc gacataggtg cgcggcacgc gtttgctcat c
//
LOCUS       DASVOR010000167         4667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_264536,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000167 DASVOR010000000
VERSION     DASVOR010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4667
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264536"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VF516_03750"
     CDS             complement(<1..1183)
                     /locus_tag="VF516_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX8106813.1"
                     /translation="MAGRASIVGAAATHVGNVRDHNEDAHFLDEDLGVFLVCDGMGGH
                     AAGEVASELAIGAIRTAWGSPALHEAAEQWLERATAEAKKQLLDVVRGGVVSAHEAIL
                     AEADRDQTKSGMGTTLVGAVIVGGELVFAHAGDSRAYLVRDGISMQLTEDHTLLARLL
                     AAGIDVDVSGEGARFRSMLTNALGIGEEVKVSTFVVPLADGDRFLLCSDGISEYVPEH
                     EIGEVLTRQSNPARAAQRLIDLALERGGGDNATAVVVRVLEAGETPLPAEQRKKDDAA
                     ISACALWGKKLTPQQRLRALRIAIPRDHGLGEKLPPHALGDRVAWIILEGELVQDGET
                     FGPGALIYPESLVSTRESAPPPGRDALAAARSDVRALAIRANDFRELCDDDSELGEAL
                     LD"
     gene            complement(1198..1905)
                     /locus_tag="VF516_03755"
     CDS             complement(1198..1905)
                     /locus_tag="VF516_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8106814.1"
                     /translation="MRVIVICAEPERAERPDRPDHPDESGTVAGLLGELGCRVTLGRF
                     DLGGLDLDAVAEQPPSVVIVDAGEDVGRAHKSIKRMRDDGPLVEVPILIAVTVARLPA
                     LDFSIGFDDFVLTPIVPAELYARLRQLDWKTATFGSDEILKIDDLVIDIAGYEARLAG
                     RRIELTHQEFELLRFLGQHRGRVFTREALLERAWGYRYAGGTRTVDIHVRRVRSKLGE
                     AGSIIETVRNVGYKMRG"
     gene            2034..3242
                     /gene="clpX"
                     /locus_tag="VF516_03760"
     CDS             2034..3242
                     /gene="clpX"
                     /locus_tag="VF516_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX8106815.1"
                     /translation="MEEFHCSFCGKQRREVRKLISGPRVFICDQCVTLCNDILAKEEA
                     SERPKYPPPRAIVEELDRYVVGQKDAKRALSVAVSNHYKRIGLRGKQTDVELQKGNIL
                     LIGPTGSGKTLLAQTLAKKLDVPFAIADATTLTEAGYVGEDVESVVKALFRAAGGDVE
                     KTARGIICIDEIDKIARKGGTPSPTRDVSGEGVQQALLKSLEGKTATITPDGARNRPQ
                     QELIQIDTTDILFICCGAFNGLEDIVRRRTGQRGLGFGAQVSTAEDDKDALRRQARTE
                     DLIKFGMIPEFMGRLPVIVSCDALDVEALTEVLWKPKNALAKQYHRLFELEGVKLNFR
                     PDALTAIAEEAARRNSGARGLRAIVEEVMLDVMYEIPSLTGVKECVVTREVVERRARP
                     ELVLESKAAS"
     gene            complement(3328..>4667)
                     /locus_tag="VF516_03765"
     CDS             complement(3328..>4667)
                     /locus_tag="VF516_03765"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8106816.1"
                     /translation="FVSLVRVSGQTPFATGCGGGQQGTNFGSVEVEPWVAVDPTNPAH
                     LVGAWQQDRWSNGGANGIGTAATFDGGATWSTHTPRIGRCAGGTAGGDYQRASDPWVT
                     FAADGTVFLIALVFDATTPRNAVVASRSTDGGATWSDPAVLFADDDPDFFSDKEAITA
                     DPTDPGRVYAVWDRLTGQTHPTDPTNTGPTWFARTTDGAWEPARAIFEPGADAQTLGN
                     VIAVLPGGTLVDVFDLITQATSMAPMNMLGVIRSEDHGLTWSAATVIAPMRGVGVQDP
                     DSHVGIRSGTDLPQVAVDRTTGALYIVWQDAPAGGTVDAVMLVSSQDGGVTWSAPVAV
                     NGAPGAAAFTPSVAVAADGTVGVTYFDLRNAKPGDDAVRVTPWLATSRDRGATWSDEA
                     LSAPFDLHPALLQGFYFLGDYQGLVAVGNGFVPFFTAAVADGDDRTDVFVHLP"
BASE COUNT          684 a         1781 c         1546 g          656 t
ORIGIN      
        1 tgtcgagcag cgcctcgccg agctcgctgt cgtcgtcgca cagctcgcgg aagtcgttgg
       61 cgcggatcgc cagcgcgcgg acgtcgctgc gcgccgccgc cagggcatcg cgtcccggcg
      121 gcggcgccga ctcgcgcgtg ctgaccagcg actcgggata gatcaacgcg ccggggccaa
      181 acgtctcgcc gtcctggacc agctcgcctt ccaggatgat ccacgccacg cgatcgccga
      241 gcgcgtgcgg cggcagcttc tcgccgagcc cgtgatcgcg cgggatcgcg atccgcagcg
      301 cgcgcagccg ctgctgcggc gtcagcttct tgccccacag cgcgcacgcg ctgatcgcgg
      361 cgtcgtcctt cttgcgctgc tcggcgggca gcggggtctc gccggcctcg agcacccgga
      421 ccacgaccgc ggtcgcgttg tcgccgccgc cgcgctcgag cgcgaggtcg atcagccgct
      481 gcgccgcgcg cgccgggttc gactgcctgg tcagcacctc gccgatctcg tgctcgggca
      541 cgtactcgct gatcccgtcg ctgcacaaaa ggaagcgatc tccgtcggcc agcggcacca
      601 cgaaggtcga gaccttgacc tcctcgccga tcccgagcgc gttggtcagc atgctgcgga
      661 accgcgcgcc ctcgcccgac acgtcgacgt cgatccccgc ggcgagcagc cgggcgagca
      721 gcgtgtgatc ctcggtcagc tgcatcgaga tcccgtcgcg caccaggtag gcgcgcgagt
      781 cgccggcgtg cgcgaacacc agctcgccgc cgacgatcac cgcgccgacc agcgtggtgc
      841 ccatcccgct cttggtctgg tcgcggtcgg cctcggcgag gatcgcctcg tgcgcggaga
      901 ccacgccgcc gcgcaccacg tccagcagct gcttcttggc ctcggccgtg gcgcgctcca
      961 gccactgctc cgcggcctcg tgcagcgccg gactgcccca cgccgtgcgg atcgctccga
     1021 tcgccagctc gctcgcgacc tcgccggcgg cgtgccctcc catcccgtcg cataccagga
     1081 atacaccgag atcctcgtcg aggaaatgcg cgtcctcgtt gtgatcgcgc acgttcccca
     1141 catgggtcgc cgcagcgccg acgatcgagg ctcgcccggc cactggttca ctgtacacta
     1201 gccgcgcatc ttgtagccga cgttgcgcac cgtctcgatg atcgagccgg cctcccccag
     1261 cttggatcgg acccggcgaa cgtggatgtc gaccgtgcgc gtcccgccgg cgtagcggta
     1321 gccccaggcc cgctccagca gcgcctcgcg cgtgaacacc cggccgcggt gctggccgag
     1381 gaaccgcaac agctcgaact cctggtgggt cagctcgatc cgccggccgg ccagccgcgc
     1441 ctcgtagccc gcgatgtcga tcacgaggtc gtcgatcttc aggatctcgt cggagccgaa
     1501 cgtcgcggtc ttccagtcga gctggcgcag ccgggcgtag agctcggccg ggacgatcgg
     1561 cgtcagcacg aagtcgtcga acccgatcga gaagtcgagc gccggcagcc gcgccacggt
     1621 gacggcgatc aagatcggaa cttcgacgag cggcccgtcg tcgcgcatcc gcttgatcga
     1681 cttgtgcgcg cggccgacgt cctcgccggc gtccacgatc accacggagg gcggctgctc
     1741 ggcgaccgcg tccagatcga gcccgccgag gtcgaaccgg ccgagcgtca cgcggcaccc
     1801 cagctcgcct agcaatcctg cgaccgtgcc ggattcatcc gggtggtcgg ggcgatcggg
     1861 ccgttcggca cgctcgggct cggcacagat cacgataacg cgcacggtcg aagtgatccc
     1921 cttgccaccg ctcccgggtc aagagacagc agaggtgtac ttcacggtcg cacccgagac
     1981 gaggcgctcc ggtctattgc ctgggccccg aaacatgtaa gaattggagc tggatggaag
     2041 agttccactg ctccttctgc ggcaagcagc ggcgcgaggt gcgaaagctc atcagcggcc
     2101 cccgcgtgtt catctgcgac cagtgcgtga ctctttgcaa cgacatcctc gccaaggaag
     2161 aggcgtcgga gcggcccaag tatccgccac cacgcgcgat cgtggaggag ctcgaccgct
     2221 acgtcgtggg ccagaaggac gccaagcgcg ccctgtcggt cgcggtctcc aaccactaca
     2281 agcgcatcgg cctgcggggc aagcagaccg acgtcgagct ccagaagggc aacatcctcc
     2341 tgatcggccc caccggttcg gggaagaccc tgctcgccca gaccctggcc aagaagcttg
     2401 acgtcccgtt cgcgatcgcc gatgccacca cgctcaccga ggccggctac gtcggcgagg
     2461 acgtcgagag cgtggtcaag gccctgttcc gcgccgccgg cggcgacgtc gagaagaccg
     2521 cgcgcggcat catctgcatc gacgagatcg acaagatcgc ccgcaagggc ggcacgccgt
     2581 cgccgacccg cgacgtctcg ggcgagggcg tccagcaggc cctgctcaag agcctcgagg
     2641 gcaagaccgc gacgatcacc ccggacggcg cgcgcaaccg gccgcagcag gagctgatcc
     2701 agatcgacac cacggacatc ctgttcatct gctgcggcgc gttcaacggc ctggaggaca
     2761 tcgtccgccg ccgcaccggc cagcgcggcc tggggttcgg cgcccaggtg tcgaccgccg
     2821 aggacgacaa ggacgcgctg cgccgccagg cccgcaccga ggacctgatc aagttcggca
     2881 tgatccccga gttcatgggc cggctgccgg tgatcgtctc gtgcgacgcg ctcgacgtcg
     2941 aggcgctgac cgaggtgctg tggaagccca agaacgcgct cgccaagcag taccaccggc
     3001 tgttcgagct cgagggcgtc aagctcaact tccggcccga cgcgctgacc gcgatcgccg
     3061 aggaggccgc gcgccgcaac tccggtgcgc gcggcctgcg cgcgatcgtc gaggaggtca
     3121 tgctcgacgt catgtacgag atcccctcgc tcaccggcgt caaggagtgc gtcgtgaccc
     3181 gcgaggtcgt cgagcgccgc gcccgccccg agctcgtgct cgagagcaag gccgcgagct
     3241 gatccgcgcc gatcgcccgg tcttctcgtg ccggtcgggc cgggcgggcg tgcgctgccg
     3301 cccgggatcg ccgcgtgcgg ccgcgcgcta tggcaggtgc acgaacacgt cggtccggtc
     3361 atcgccatcg gcgaccgccg cggtgaagaa cgggacgaac ccgttgccca ccgcgaccag
     3421 cccctggtag tcgccgagga agtagaaccc ctgcagcagc gccgggtgga ggtcgaacgg
     3481 cgcgctcagc gcctcgtcgc tccacgtcgc cccgcgatcg cgtgaggtcg ccagccacgg
     3541 cgtcacccgc accgcgtcgt cgcccggctt cgcgttgcgc aggtcgaagt acgtcacccc
     3601 gaccgtgccg tcggccgcga ccgccacgct cggcgtgaac gccgccgcgc ccggcgcccc
     3661 gttgaccgcc accggcgccg accacgtcac cccgccgtcc tgcgagctca ccagcatcac
     3721 cgcgtcgacc gtgccgcccg ccggcgcgtc ctgccacacg atgtagagcg ccccggtcgt
     3781 gcgatccacc gcgacctgcg gcaggtcggt ccccgagcgg atccccacgt ggctgtccgg
     3841 atcctggacc ccgacccccc gcatcggcgc gatcaccgtc gccgccgacc aggtcagccc
     3901 gtggtcctcc gagcggatca cgccgagcat gttcatcggc gccatgctgg tggcctgcgt
     3961 gatcaggtcg aacacgtcga ccagcgtccc gccgggcagc accgcgatca cgttgccgag
     4021 cgtctgcgca tcggccccgg gttcgaagat cgcccgcgcc ggctcccacg ccccgtcggt
     4081 cgtgcgcgcg aaccacgtcg gcccggtgtt ggtcgggtcg gtcgggtggg tctggccggt
     4141 cagccggtcc cacaccgcgt agacccggcc cgggtccgtc ggatccgccg tgatcgcctc
     4201 cttgtcgctg aagaagtcgg gatcgtcgtc ggcgaacagc accgccggat cgctccaggt
     4261 cgcgccgccg tcggtcgacc ggctcgccac caccgcgttg cgcggcgtcg tcgcgtcgaa
     4321 caccagcgcg atcaggaaca ccgtcccgtc ggccgcgaac gtcacccacg gatcgctcgc
     4381 gcgctggtag tcgccgccgg ccgtcccccc ggcgcaccgg ccgatccgcg gcgtgtgggt
     4441 cgaccacgtc gccccgccgt cgaacgtcgc cgcggtcccg atcccgttcg cgccgccgtt
     4501 cgaccagcgg tcctgctgcc acgcgccgac caggtgcgcc gggttcgtcg gatccaccgc
     4561 gacccacggc tcgacctcga cgctgccgaa gttcgtcccc tgctgcccgc cgccgcagcc
     4621 ggtggcgaac ggcgtctggc ccgacacccg gaccagcgac acgaacg
//
LOCUS       DASVOR010000168         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439936,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000168 DASVOR010000000
VERSION     DASVOR010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439936"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VF516_03770"
     CDS             <1..1585
                     /locus_tag="VF516_03770"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8106817.1"
                     /translation="GVLYIASAKFYFMLAGLVIQVRLPAVLSRAAFGSFSLVSNIASL
                     VNNVLVTGTIQAVSRFAAQEPGKARRVQQAGLRMHVRLGLVIAVGFIAAAPLVAWLLH
                     DMTKTAPLMLAGLIVGGYSFYAVFVGTANGLHQFHKQAGLDMTFATLRVAGLLGMAMA
                     GFGVIGVIGGWVAAVGLILCAAVVWVGLPGPVAREDRLPVRPMIEFFLGVALYLVLFN
                     ALMFVDSILIKRLTTEHYAAHLGALDGALAGALPWAAAATGYRPDPSVLADVQNGYYA
                     AVQNLARLPYQAIIAATFVVFPLVSRSTFTEDRETTRRYIQITTRYSLIFAMALAVVM
                     AADPRDMLGLVYAPDYAELGAPALTALALGNVAFSIFAIAGTILNGAGRTLPAVVTAA
                     LTLALAAVGNYVAIPIAAEHGSVLLVAAVVTSGAMLVGALASGWALRRELGAFLPVAS
                     VVRVAIATAAAIAVDRGRLAERAIGAVLPIHGKPMTLIQAMVVGAAFLAVLVITRELG
                     RRDLEAIQAVRRSRARNEV"
     gene            1618..2091
                     /locus_tag="VF516_03775"
     CDS             1618..2091
                     /locus_tag="VF516_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106818.1"
                     /translation="MRAALLAILMLGCGASLHDQPDLSESVRMFNDGVRWERFTAAAA
                     ALPPRERSQFVDDMDQRSGDVKITDYEVMRIDPRGEQEARVQVKLSWYKTSEGTIHET
                     HAVQTWERHGKAWWMVDESRLRGAPMPGLSESPEPPATPVSVTPVAATPGSVMKD"
     gene            2150..>2587
                     /locus_tag="VF516_03780"
     CDS             2150..>2587
                     /locus_tag="VF516_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106819.1"
                     /translation="MSQSDFVTRGQALVSSGQYQEAVKVCRLGLLGRPTTVEGRVVLG
                     QALLALKRYDEVLAEMRVALELDHASHAAQVLKGEALLRKGDGHGAAEVLGKLRSQGL
                     ADTHVAELLAEAERLASRSPPGNANPSVGFLAQEAASFAAEAST"
BASE COUNT          333 a          920 c          944 g          390 t
ORIGIN      
        1 cggggtgctg tacatcgcgt ctgcaaagtt ctacttcatg ctcgccggcc tggtgatcca
       61 ggtccggctg ccggcggtgc tgtcgcgcgc ggcgttcggc agcttcagcc tggtgtcgaa
      121 catcgcgtcg ctggtcaaca acgtgctggt caccggcacg atccaggcgg tgtcgcggtt
      181 cgccgcccag gagccgggca aggcgcggcg ggtccagcag gccgggctgc gcatgcacgt
      241 ccggctcggc ctggtgatcg cggtcggctt catcgccgcg gcgccgctgg tcgcgtggct
      301 gctccacgac atgaccaaga ccgcgccgct gatgctcgcc ggcctgatcg tcggcggcta
      361 ctcgttctac gccgtgttcg tcggcaccgc gaacggcctg caccagttcc acaagcaggc
      421 cgggctcgac atgacgttcg ccacgctgcg ggtcgccggc ctgctcggca tggcgatggc
      481 cgggttcggc gtgatcggcg tgatcggcgg ctgggtcgcc gcggtcggcc tgatcctgtg
      541 cgccgcggtg gtgtgggtcg ggctgcccgg cccggtcgcg cgcgaggacc ggctgccggt
      601 gcggccgatg atcgagttct tcctcggcgt cgcgctctac ctcgtgctgt tcaacgcgct
      661 gatgttcgtc gacagcatcc tgatcaagcg gctgaccacc gagcactacg ccgctcacct
      721 cggggcgctg gacggcgcgc tcgccggcgc gctgccgtgg gcggccgccg cgaccggcta
      781 tcgccccgat cccagcgtgc tcgccgacgt ccagaacggc tactacgccg cggtccagaa
      841 cctggcgcgg ctgccgtacc aggcgatcat cgccgcgacg ttcgtcgtgt tcccgctggt
      901 gtcgcgctcg acgttcaccg aggaccgcga gacgacgcgg cgctacatcc agatcaccac
      961 gcggtactcg ctgatcttcg cgatggcgct cgcggtggtg atggccgccg acccccgcga
     1021 catgctcggc ctggtctacg cgcccgacta cgccgagctc ggcgcgccgg cgctgaccgc
     1081 gctcgcgctc ggcaacgtcg cgttctcgat cttcgcgatc gccggcacga tcctcaacgg
     1141 cgcgggccgg accctgccgg cggtggtcac cgccgcgctc acgctcgccc tggcggcggt
     1201 cggcaactac gtcgcgatcc cgatcgcggc cgagcacggc agcgtgctgc tggtcgccgc
     1261 cgtggtcacc agcggcgcga tgctcgtcgg cgcgctggcg tcgggctggg cgctgcgccg
     1321 cgagctcggc gcgttcctgc cggtcgccag cgtcgtccgg gtcgcgatcg cgaccgccgc
     1381 ggcgatcgcg gtcgaccgcg gtcggctcgc cgagcgcgcg atcggcgccg tgctgccgat
     1441 ccacggcaag ccgatgaccc tgatccaggc aatggtggtc ggtgcggcgt tcctcgccgt
     1501 gctggtgatc acgcgcgagc tcggccggcg cgatctggag gcgatccagg cggtgcggcg
     1561 aagccgcgcg cgcaacgagg tctagacgcc tggagaggcc caggcgagga gccaaccatg
     1621 cgagcggcac tacttgcgat cctgatgctg ggctgcggcg catcgctgca cgaccagccc
     1681 gatctgtccg agtcggtccg gatgttcaac gacggcgtgc gctgggagcg cttcaccgcg
     1741 gcggccgccg cgctgccgcc gcgcgagcgc tcgcagttcg tcgacgacat ggaccagcgg
     1801 tcgggcgacg tcaagatcac cgactacgag gtcatgcgga tcgacccgcg cggcgagcag
     1861 gaggcccggg tccaggtcaa gctcagctgg tacaagacct ccgagggcac gatccacgag
     1921 acccacgcgg tccagacctg ggagcgccac ggcaaggcct ggtggatggt ggacgagtcc
     1981 cggctgcgcg gcgcgccgat gccgggcctg tccgaatccc cggaaccgcc cgcgacgccg
     2041 gtttccgtga cgccggttgc ggcgacgcca ggttcagtga tgaaggatta ggaccacatc
     2101 cgccgcgatt tggccggcgc ggcgccggtc gaggtaagct tggcaccagg tgtcgcagag
     2161 cgacttcgtc acgcgcggcc aggcgctggt cagctccgga cagtaccagg aggccgtcaa
     2221 ggtctgccgg ctcggcctcc tggggcggcc gaccacggtg gagggccgcg tcgtgctcgg
     2281 ccaggcgctg ctcgcgctca agcgctacga cgaggtgctg gccgagatgc gggtcgcgct
     2341 cgagctcgat cacgcgtcgc acgccgccca ggtgctcaag ggcgaggcgc tgctgcgcaa
     2401 gggcgacggc cacggcgccg ccgaggtgct gggcaagctg cgcagccagg ggctcgccga
     2461 cacgcacgtc gccgagttgc tcgccgaggc cgagcggctg gcgagccggt cgccgccggg
     2521 aaacgcgaat ccgtcggtcg gcttcctcgc gcaggaggcc gcgtcgttcg ccgccgaggc
     2581 aagcacc
//
LOCUS       DASVOR010000169         4213 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_323143,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000169 DASVOR010000000
VERSION     DASVOR010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323143"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="VF516_03785"
     CDS             <1..230
                     /locus_tag="VF516_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106820.1"
                     /translation="AEAEARRQAAAAERERKQAELAAERERRRQAAEEARANRSSGGL
                     LCNDGTLSPTCTCAGSHRGCCSWHGGVAGCQ"
     gene            complement(481..996)
                     /locus_tag="VF516_03790"
     CDS             complement(481..996)
                     /locus_tag="VF516_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106821.1"
                     /translation="MTQLKRGADLVEVMTADGMLDTETTPGLLSMFGIITGSVANFHC
                     FMPWRTLWDAVVDEAQPSVPSPIEDAIFAASLRTSWGVLGLMAHDSQSWLIPASRHRP
                     LLDAALEVWPALDAVGERYAVGSMPQRLWSVPNNLHFVLANLGVPNDMLGAPLPPEGP
                     RALLRYVRPVN"
     gene            complement(1062..2936)
                     /locus_tag="VF516_03795"
     CDS             complement(1062..2936)
                     /locus_tag="VF516_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8106822.1"
                     /translation="MCPGARQLRARHPEPRCRSLPSASRGAHWARNRQSLCPSQHLDE
                     LAMGGGRDQRQGAGGQRGPHGASSSGFAPGKRTLTEQLPVQCSTGSVPALVDTEASAV
                     QHAATQGVASAGSSLPHADTIQRLFGRHDIRGVEAHMGGEAAVATHAIGAEAYATGHH
                     VAFASSPSLHTAAHEAAHVIQQRGGVQLKDGVGWSGDAHEQHANAVADLVVQGKSAEA
                     SLDGYASAGGGPAAVQRKPDDVPSLQRPEGARVDRLIQLLAAPAAAAAGRDDVYMLLS
                     SLGIPELLAVMEGVADRGYLPQLHARVSSWTNPISVARLLSALYAVELVRVPPDAVAT
                     GQLKAAGMALDLLPHDQQVQIVEYVLHHRGAGVSVTEVFEGALAMREGKDAARDPQVH
                     GGSAATAGPMATAATGSAPAPVEPDPWAPPGEQPIPFYIGNAAHIGIAASYVAAHGGE
                     VVRTNASPVGSMLTTLKRTLGAQGEKIDKGALSEDELALMPDITNLTRLHLYEIKPLA
                     AEAAGAAKAAMYASLFAKAGVAVALGPTTEPGVEGGIPAPAGVYMFWSPQPGVIVYQY
                     RKGRLVPVPVGEPEPAGERRWKFELRPMTPQQQAAVTTLTVGGMLLLIAMILLAPVGA
                     "
     gene            complement(3138..3359)
                     /locus_tag="VF516_03800"
     CDS             complement(3138..3359)
                     /locus_tag="VF516_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106823.1"
                     /translation="MFEIRVADPDDFLGWPTLLEIMPSDSVRREEVVPAVTSLMNALI
                     ARGRRVLAQCDYAEDLPGGGEVAGPTATP"
     gene            complement(3390..3512)
                     /locus_tag="VF516_03805"
     CDS             complement(3390..3512)
                     /locus_tag="VF516_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106824.1"
                     /translation="MNDALFCSIHVDSSLSRDAMASLVAELTGGVVVLGGVDCA"
BASE COUNT          616 a         1517 c         1461 g          619 t
ORIGIN      
        1 gcgccgaagc cgaggcgcgg cgccaagctg ccgccgcgga gcgcgagcgc aagcaggcag
       61 agctcgccgc cgagcgcgag cgtcgccgcc aggccgcgga ggaagcgcgc gccaaccgat
      121 cctcgggcgg cttgctctgc aacgacggaa ccctgtcgcc gacgtgcacg tgcgccggct
      181 cgcaccgggg ctgctgctcc tggcacggcg gcgtcgccgg ctgccagtga atcgccgcct
      241 cggcgtatgt ccaccaccac ggacggacag cgcgccgcac cgacccgagg ccactgggga
      301 ccgcgacggg cgggcggtgc gcggtcgatg acctgccggc ctgcgagctc gggtgctcgg
      361 cgatctagcg ccgccaaccg gccgcgctcc atgccgcgat ccggccggcg gcacggcgca
      421 gatcgagctg ccgatcggcg agccgcgcgt ggagacggag ccgggcatcg tgccgcgccg
      481 tcagttcacc ggccgcacgt accggagcag cgcgcggggg ccctccggcg gcagcggcgc
      541 gccgagcatg tcgtttggca cgcccaagtt cgcgagcacg aagtgtaggt tgttcggcac
      601 cgaccacagg cgctgaggca tgctgccaac ggcgtaccgc tcaccgacgg cgtcgagcgc
      661 cggccagacc tcgagagcgg cgtccaagag cggccgatgt cgagacgccg ggatcagcca
      721 ggactggctg tcgtgcgcca tgagcccgag cacgccccat gacgtgcgca gcgacgctgc
      781 gaagatcgca tcctcgatcg gcgacggtac gctcggctga gcctcgtcga cgacggcatc
      841 ccagagcgta cgccacggca tgaagcagtg gaagttcgcc accgaccccg tgatgatgcc
      901 gaacatcgac agcagccccg gcgtcgtctc ggtatcgagc attccgtccg cggtcatcac
      961 ctcgacgaga tccgcgccgc gcttgagctg cgtcacgacc cgcgccatgc gcttcttgac
     1021 ggtcttccag tcgccgtact ggatcgcggg cagctgcacg gtcaggctcc gacaggcgcg
     1081 aggaggatca tggcgatcag caggagcata cctccgacgg tgagggtcgt cactgcggcc
     1141 tgctgctgcg gcgtcatcgg ccggagttcg aacttccagc gtcgctctcc ggctggctcg
     1201 ggctcgccca cgggcaccgg cacgagcctg cccttccggt actggtagac gatgacgccc
     1261 ggctgcggag accagaacat gtacacccca gccggcgccg ggatgccacc ttcgacgccc
     1321 ggctcggtcg tcggtccgag cgcgacggcg acgcctgcct tggcgaacag gctggcgtac
     1381 atcgccgcct tggcggctcc cgcggcctcc gcggcgagcg gcttgatctc gtagaggtgc
     1441 agacgcgtca ggttcgtgat gtccggcatc agcgcgagct cgtcttcgct cagcgcaccc
     1501 ttgtcgatct tctcgccctg cgcaccgagc gttctcttga gggtggtcag catgctcccg
     1561 accggagacg cgttggtgcg caccacctcg ccgccgtgag ccgcgacata gcttgccgcg
     1621 atgccgatgt gcgccgcgtt gccgatgtag aacgggatcg gctgctcgcc gggcggcgcc
     1681 caggggtcag gctcaacagg cgccggcgcg ctgccggttg cggcggtcgc catcgggccg
     1741 gcagtcgccg cgctgccgcc gtgcacctgc ggatcccgcg cagcgtcctt gccctcgcgc
     1801 atcgccagcg caccctcgaa cacctcggtg acgctgacgc cggcgccgcg gtggtgcagc
     1861 acgtactcga cgatctggac ctgctggtcg tgtggcagca gatcgagcgc catgccggcc
     1921 gccttcagct gccccgtcgc caccgcgtcc ggcggcacgc ggacgagctc gacggcgtac
     1981 agcgccgaga gcagcctcgc cacggagatc gggttcgtcc agctgctcac acgagcgtgg
     2041 agctggggca gatacccgcg atcggcgacg ccctccatca ccgcgagcag ctcggggata
     2101 ccgagtgacg acagcagcat gtacacgtcg tcgcgaccag cggctgcggc cgccggcgcg
     2161 gctagcagct ggatcaagcg gtcgacgcga gcgccctccg gccgctgcag cgacggcaca
     2221 tcgtcgggct tgcgctgcac cgccgccggt ccgccgccgg cgctggcgta cccgtcgagc
     2281 gacgcctcgg ccgacttgcc ctgcacgacc aagtcggcga ctgcgttcgc gtgctgctca
     2341 tgcgcgtcgc cgctccagcc gacgccgtcc ttgagttgca cgccgccgcg ctgctggatc
     2401 acgtgcgccg cctcgtgcgc ggccgtgtga aggctcgggg acgaggcgaa cgcgacgtgg
     2461 tgcccggtgg cgtaggcctc ggcgccgatc gcgtgggtcg cgactgcggc ttcgccgccc
     2521 atgtgagcct ccacgcctct gatgtcgtgc cggccgaaca ggcgctggat cgtgtcggca
     2581 tgcggcagcg aactgcccgc gctggcgacg ccctgcgtcg cggcgtgctg gacggcgctg
     2641 gcctcggtgt cgacgagggc cggcaccgag ccagtcgagc attgcaccgg cagctgctcg
     2701 gtgagcgtcc gtttgccggg ggcgaaaccc gacgaactcg cgccgtgagg accgcgttgc
     2761 ccgccggcgc cttgtcgctg atcgcgtcca ccgcccatgg ccaattcgtc caagtgttgc
     2821 gatgggcaga gcgactgtcg attccgcgcc cagtgcgccc cgcgtgaggc gcttggcaga
     2881 gagcggcacc ggggctctgg atggcgcgcc cggagctgcc gcgcgcctgg gcacaccaag
     2941 ggctgtctcg tggcgatcgg ggctttcggc gaccgcggtg cgcgcgggtg aggttctacg
     3001 cagccggtgc ctgaccccgc cccgtcggga gcgcctacct acctcggtac ttcggcatgg
     3061 ctactatcgc gtcgagcgcc tgggtgaggt gatagccgct ggcgcgttca aggccgctga
     3121 acattggcat tgcggtccta tggcgtcgct gtaggaccag cgacctctcc accaccggga
     3181 agatcttcag cgtagtcgca ctgagcgagc acgcgcctcc cgcgggcgat cagcgcgttc
     3241 atcagggagg tcactgcagg tacgacctct tcgcggcgca cgctgtccga tggcatgatc
     3301 tcgaggagag ttggccagcc caggaaatcg tcagggtctg ctacccggat ctcgaacatg
     3361 ccatagtcgt cgtcgacggc aatgtgtccc tacgcgcagt cgacaccgcc gagcaccacc
     3421 acgccgccgg tcaactccgc gacgagcgac gccatggcat cgcgcgacag ggagctatcg
     3481 acatggatcg aacagaacag tgcgtcattc atggtgtggt gctcagggaa tagggaagta
     3541 aggtccgtga tcgcttgcct cccaccgtga cggtacccga tctgacgtcg cgtttcgcag
     3601 cgtctcgcgg aactcacgct tggcgatata caccgagcgg cgtcgagtga gctgcacata
     3661 cgtcgactac cgtagggcag cgtcatccgc gatgacctcg ctcgatgagc ttgctagcca
     3721 gcacggcgat cgccagacgg tgtccggtgc gacgctccgc gtctccgccc acggcgagtt
     3781 cggcgaggcc cgggtcggcg gcgtgaacat cgacgcagag cagctcgacc tcgtgctcgg
     3841 cgagcccgcc ggcgacgacg ccccggcgcg ctcaccgcag cgccaggctg ccgcttgcca
     3901 gctcgagcgt gccggttcaa cgcgcgagcc gcgcggcttg ttccacgcgc gcgtgtgggc
     3961 gcgcggacac gcggcgccgt gtcgatcctg gcgatcgcgc acgcaaccat gcggcggcac
     4021 gtgtggcacg ccgcgtgctg aagcgccgcg cgatgaacgc tcgcacctgg ctcgccaccc
     4081 tgctcgtcgc cggctgcggc agcgcggaca cgctgcgcac ccagcccgcc gagcttccac
     4141 aaccgatcgc gccaccgccg accgtcgtcg acggctacca tgcggtgacc acgcccgcgc
     4201 cgccgccgcc gag
//
LOCUS       DASVOR010000170         8971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_147745,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000170 DASVOR010000000
VERSION     DASVOR010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8971
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147745"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1936
                     /locus_tag="VF516_03810"
     CDS             <1..1936
                     /locus_tag="VF516_03810"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8106825.1"
                     /translation="APRAASDGDAPPSYVLACGGRIATTGTGVDAFEPGGHVVAFATA
                     AFGAHVAAASSAAALVRERGLIPALVAAAPPYVAAHLALFGSAVLTRGERIVVRATKQ
                     GIGVAAAAWARLRGAEVYVACAGADTASIIAAGGHPLAAGTTAELVGEVGRLTAGRGV
                     DIVLDEADDADLLEEAPKLLRQRGRFVHVSLGGPRIQARIPQGSRDLVFAFTSLATSI
                     RENPSELGPILRELVARVEAGDLLLPVPAVASLAAVEAAGDAASGGWTVVRLDEPGAT
                     VAARFRGAIRGDASYLITGGLGGLGLSVAAWMVARGARNLVLVGRRGATSDAQRAALA
                     ELERAGARVLVASADVCSRRDVDDLVRRIERELPPLRGVIHAAGLVPKIRPFVELELA
                     DLTEVLGPKVVGTWHVHEATRAATLDFFVLYSSISAWIGSKEFASYPAANAFLDAFGH
                     YRRGQGVAGTSIAWGPFAEAGMALPFAERWERVVGLRSMALTEGVEILEQILRADVTD
                     VCAVRLDVARVVEALPAFKTSKLWSDVVEGATAGKRTRSQQAGELVAALRAAAPDARL
                     GLVEQFVQQQVATVLRAEADEIKPNRSLRTQGMDSLTWLELRQRVESALEVTLPVELF
                     MKDPTPSSLAAELHARSSAR"
     gene            1946..3091
                     /locus_tag="VF516_03815"
     CDS             1946..3091
                     /locus_tag="VF516_03815"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8106826.1"
                     /translation="MTDHRKTAAAFVDKHLLGREEFFDSAPELPLAVHRDLQAAGLAT
                     WWLGSDGEPAMSLEDGVDVVEELAYGDAGVAFSAFVTILVTTPVYLYGSDSQRAAIGA
                     MCERGGVAATLGSERAAGSELFRISTTAHRHGDRIHLDGQKLFSTNAGFADLMMVIAS
                     APDEPQRYVAALIPARAKGVKIVQRWDLVGVRAAGTYEVELAGCNVPADSLLQISGIK
                     ALEIGLNPSRILIAATGVGIGRRMLDLCLEYAGGKELKGGVLRDNHVFAAKIGQMEME
                     LTAMHTACKAAARDFDALMHSPDPAAAFEQAGALKSALVAKMLCGQLGWKIASVASEM
                     FGGLGFTRQMLVHKLVRDMRYISILEGGDDVLRDLLYARYVRPRGRG"
     gene            3237..4511
                     /locus_tag="VF516_03820"
     CDS             3237..4511
                     /locus_tag="VF516_03820"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8106827.1"
                     /translation="MAIILCALVAACSHPSQPQDPRPSTVTAFVDVTVVAPRVGTAAA
                     HQTVLVDGERIVRVGPTAEVGVPAGARIVAAAGRFLVPGFADMHVHLPDTQPEIDRVL
                     DLSLAAGVTTIRSMQGKPGHLEARARLARDHRLAPELVLAGPPITEALTPDQVRELVR
                     DQKRAGYDLIKLLGGIDRATYDALVAEAAAQHIPVVGHVPAQIGLAAALDARQRTIEH
                     VQGYIAAAHDDPAQLAELARRTREAGVFNCPTLDFYAVALGPRDHLAERAGISGYTTA
                     DELATWTRALAERAVPADAAGRLAKIAAVVVALRDAGAPILVGSDTPDTFALPGFGYV
                     EELHALARVGLSPADVLRAATASAAAALGRPDDGAVAGARADLVLLERDPLASVENLA
                     HPAGVMVRGRWSTRADLDALLAAHRTSAAAPE"
     gene            4785..4976
                     /locus_tag="VF516_03825"
     CDS             4785..4976
                     /locus_tag="VF516_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106828.1"
                     /translation="MKKFSFKSAPRTLTAEKRAMREQMLRAVPEELLSQIRGGGLEDG
                     CHSAFCCCDDGCSLSILQG"
     gene            5026..6210
                     /locus_tag="VF516_03830"
     CDS             5026..6210
                     /locus_tag="VF516_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106829.1"
                     /translation="MSVDAFMGWLADATRDGQAHLADTLRALVYRADPELFDVLEDVD
                     QDVFLEPLLFAHFAVNQPSIPLPQIVVGYADAVMRPEAVEVRSDAAGTIYLPRIGYFL
                     TEARDRTLVLHWDAETGRASVLDGDTPIDFRFEELPTIPGTDIEVCRHGLPMLADVVA
                     AATTHGPEFWVVDDRPDGHLDHLATAMEIIRAQWPTYHAQILSTVRQVVLFRSATLNS
                     FASVSAHGVACLNVSARDDEVFFVEDLLHQCGHVMFNAATARRKEYVAVDPDLPIGPF
                     NGHPDDSRSVYVVLHGMMTEFAMVHGLRLCDERGTFGGRKAHELTGRLAFITHKALSD
                     MPSLANASLLTAKGRSLYETFAAAFEGIRRERSDLLTGYTMANQPYSFSYDVFTRLNP
                     MP"
     gene            6644..6808
                     /locus_tag="VF516_03835"
     CDS             6644..6808
                     /locus_tag="VF516_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106830.1"
                     /translation="MKKKNDKKSLPKFKAVTLGADELSRAVGGLDCTCGTVSVCHVDG
                     TDDGDGGLEI"
     gene            6922..7911
                     /gene="gwsG"
                     /locus_tag="VF516_03840"
     CDS             6922..7911
                     /gene="gwsG"
                     /locus_tag="VF516_03840"
                     /inference="COORDINATES: protein motif:HMM:TIGR04192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="grasp-with-spasm system ATP-grasp peptide
                     maturase"
                     /protein_id="HEX8106831.1"
                     /translation="MILIFSTAGDSSTLDVMRWIKHLSNAKVVRINADETYSVDLTFS
                     EDDYHIRVDDDAFCLADVSSAWLRKGDFWFRDLFHPVVAPWSSALSSHLSRKLQREDR
                     NLRDHFHYVLQKRCAVLGSAKGSSPNKLVVLEHAREVGLRTPPFSVSTSRQHAQRLLE
                     SDCTYVSKAMSDGLYLFDTSESKAGYFTYTEALEPHSLVGVGERMAPSFLQRYIEKKL
                     ELRIFFLDRMFLAVAIFSQADDQTRVDYRRYNMEKPNRVIPFRLPQEIAHKLSLLSDR
                     LGLNTGSIDMIVDKEDQFYFLEVNPVGQFAGHSETCNLSIERLIAERLLAYEQ"
     gene            7940..8101
                     /locus_tag="VF516_03845"
     CDS             7940..8101
                     /locus_tag="VF516_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106832.1"
                     /translation="MTERLPLGYRRILVSDDILDDEATERLTFEHSKYRHVSTHVRRP
                     LKPISKVEL"
     gene            8113..>8971
                     /locus_tag="VF516_03850"
     CDS             8113..>8971
                     /locus_tag="VF516_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106833.1"
                     /translation="MEKVLADSLGKHVLLFTDCVPVEGHTASAIYDLTRGTISTFPSE
                     YFPLFKLFRTHRLGQLLDTFEEDDRANLHDFLGFLLSNEYVAFVDDLAPFPEIPSAWD
                     APCSIENAIIDVNTQRHDYRKIVSELDALGCLHLQVRSYSPVFTVSDLVDLARLCRGT
                     SIQTLQAVIPHDPTTSDDDYVSVVTSNRILVGLVVHSASQDRRIRVDYGARGASAKLV
                     ALEIEMTTKKLESDLDCGSITKKQLLRPSTPTFNELHHFNGCLNRKISIDENGQVRNC
                     PAMGRSFGHH"
BASE COUNT         1450 a         3019 c         2983 g         1519 t
ORIGIN      
        1 agccccgcgc gccgcgagcg acggcgacgc tccaccctcg tacgtgctcg cgtgcggcgg
       61 ccggatcgcg acgaccggaa caggcgtcga tgcgttcgag ccaggtggcc acgtcgtcgc
      121 gttcgcgact gccgcgttcg gcgcgcacgt ggcggcggct tccagcgccg ctgcgctcgt
      181 acgcgagcgc ggcctgatcc ccgcgctcgt cgccgccgcg cccccgtatg tcgccgcgca
      241 tctcgcgctg ttcggtagcg ccgtgctgac acgcggcgaa cggatcgtcg tgcgcgcgac
      301 gaaacagggc atcggcgtcg cggccgcggc gtgggcgcgc ctgcgcgggg ccgaggtcta
      361 cgtcgcgtgc gccggtgccg acacggcttc catcatcgct gccggcggcc acccgctcgc
      421 cgctggcacg accgcagagc tcgtcggcga ggtcgggcgc ctgaccgccg gacgtggcgt
      481 cgacatcgtg ctggacgagg cggacgacgc cgacctcctg gaggaggcgc cgaagctgct
      541 gcgccagcgc ggccgcttcg tccacgtgag cctggggggg ccgcgcatcc aggcgcgcat
      601 tccgcagggt tcgcgcgacc tcgtgttcgc gttcacgagc ctggccacgt cgatccgcga
      661 gaacccctcg gagctcggac cgatcctgcg cgagctcgtc gcccgcgtcg aggccggaga
      721 cctcctgctg ccggtgccgg cggtcgcatc gctcgccgcg gtcgaggcgg caggcgacgc
      781 ggcttccgga ggctggacgg tggtccgtct ggacgagccc ggggccacgg tcgcggcacg
      841 ctttcgcggc gccatccgcg gcgatgcgag ctacctcatc acgggcgggc tcggcggtct
      901 cgggctttcg gtcgcggcct ggatggtcgc gcgtggcgcg cgtaacctcg tgctcgtcgg
      961 ccgccgcggc gcgacgagcg acgcccagcg cgcggcgctc gcggagctcg agcgagcggg
     1021 cgctcgggtg ctcgtcgcaa gcgccgacgt ttgctcgcgg cgagacgtcg atgacctcgt
     1081 gcgcaggatc gagcgcgagc ttccgccgct gcgtggcgtg atccacgcgg caggtctcgt
     1141 cccgaagatc cgcccgttcg tcgagctcga gctcgccgac ctgaccgagg tgctgggtcc
     1201 caaggtcgtc ggcacgtggc acgtccacga ggcgacgcgc gcggcgacgc tcgacttctt
     1261 cgtcctctac tcgtcgatat cggcgtggat cggctcgaag gagttcgcct cgtaccccgc
     1321 ggcgaacgcc ttcctcgatg cattcggcca ctatcgccgc gggcaaggcg tcgccggcac
     1381 cagcatcgcg tggggaccgt tcgcggaggc aggcatggcg ctcccgttcg cggagcgctg
     1441 ggagcgcgtc gtcggccttc ggtcgatggc tctcacggaa ggagtcgaga tcctcgaaca
     1501 gatcttgcgc gcggacgtca ccgacgtgtg tgcggtacgg ctcgacgtcg cgcgtgttgt
     1561 cgaggcactg cccgcgttca agacgtcgaa gctgtggtcg gatgtcgtcg agggcgcgac
     1621 cgcgggaaag cgcacgcggt cgcagcaggc gggtgagctc gtcgcggcgc tccgcgcagc
     1681 agcaccggat gcccggctcg ggctcgtcga gcagttcgtg cagcaacagg tcgcgacggt
     1741 gctgcgagca gaggccgacg agatcaagcc caaccgctcg ctgcgtacgc agggcatgga
     1801 ctcgctgacc tggctggagc tgcgacagcg cgtcgagtcc gcgctcgaag tgacgttgcc
     1861 cgtggagctg ttcatgaaag atccaacgcc gagcagcctc gccgccgagc tacacgcgcg
     1921 ttccagcgcg aggtagtgaa gtgacatgac cgaccatcgc aagaccgccg ccgccttcgt
     1981 cgacaagcac ctgctcggcc gcgaggagtt cttcgactcg gcgcccgagc tgccgcttgc
     2041 ggtccatcgc gatctgcagg ccgccggcct cgcgacgtgg tggctcggaa gcgatggcga
     2101 gcccgcgatg tcgctcgaag acggcgtcga tgttgtggag gagctcgcgt acggtgatgc
     2161 gggcgtcgcg ttctcggcgt tcgtcacgat cctcgtgacg acgcccgtct acctgtacgg
     2221 gtccgacagc cagcgcgcgg cgatcggcgc gatgtgcgag cgcggcggcg tagcggctac
     2281 gctcggcagc gaacgcgccg ccggaagcga gctgttccgc atctcgacca ccgcgcaccg
     2341 ccatggcgac cgcatccacc tcgacggaca gaagctgttc tcgacgaacg cgggattcgc
     2401 ggacctgatg atggtgatcg cgagcgcgcc cgacgagccg cagcgttacg tcgcggcgtt
     2461 gatcccggcg cgtgccaaag gggtgaagat cgtccagcgc tgggatctcg tcggcgtgcg
     2521 cgcggccggc acctacgagg tcgaactcgc tggttgtaac gtgccggcgg actcgcttct
     2581 ccagatcagc ggcatcaagg cgctcgagat cgggctcaac ccgagccgca tcctcatcgc
     2641 ggccaccggt gtcggcatcg gccgccgcat gctcgatctc tgcctcgaat atgccggtgg
     2701 caaggagctg aaagggggcg tattgcgcga caaccacgtg ttcgcagcga agatcggtca
     2761 gatggagatg gagctgaccg cgatgcacac cgcctgcaag gcggccgcgc gcgacttcga
     2821 cgcgctgatg cacagccccg acccggccgc tgccttcgag caggccggcg cgctcaagtc
     2881 ggcgctcgtc gccaagatgc tctgtggcca gctcggctgg aagatcgcga gcgtcgcctc
     2941 ggagatgttc ggcgggctcg gtttcacgcg ccagatgctc gtgcacaagc tcgtgcgcga
     3001 catgcgctac atctcgatcc tcgagggcgg cgacgacgtg ctgcgagatc tactgtatgc
     3061 gcgctacgtc cgtccgcgcg gccgcggcta ggtcagttgc caatgtcgac gacggcccag
     3121 cgatggccga gcccgggaca ccgacagcgg cgcgaccgtc gcgggaatga cctggaccga
     3181 gcgttgcccc gcgcgacacc agacggcaga tggtgcagga tccccgcgtg aggtccatgg
     3241 cgatcatcct ctgcgcgctg gtcgcggcgt gcagccaccc atcccagcct caggacccgc
     3301 gcccgtcgac cgtgacggcg ttcgtcgacg tcacggtcgt ggcgccacgc gtgggcaccg
     3361 cggccgcaca tcagaccgtc ctggtcgatg gtgagcggat cgtacgggtc ggccccacgg
     3421 ccgaggtcgg cgtgcccgcc ggcgcccgca tcgtggccgc ggccgggcgc ttcctcgtcc
     3481 ccggctttgc cgacatgcac gtccacctgc cggacaccca gcccgagatc gatcgcgtcc
     3541 tcgatctatc gctcgccgcg ggcgtcacca cgatccgcag catgcagggc aagccaggtc
     3601 acctcgaggc acgcgcccgc ctcgcgcgcg atcaccgcct ggcgcccgag ctcgtcctcg
     3661 ccgggccgcc gatcaccgag gccctgacgc ccgaccaggt gagggagctg gttcgcgacc
     3721 agaagcgcgc cggctacgat ctgatcaagc tgctcggcgg catcgatcgc gccacctacg
     3781 acgcgctcgt cgccgaggcg gcggcgcagc acatcccggt cgtcggccac gtgccggccc
     3841 agatcgggct cgcggccgcg ctcgacgcac ggcagcgcac gatcgagcat gtccagggct
     3901 acatcgccgc agcgcatgac gatccggccc agctcgcgga gctcgcgcgg cgtacccgcg
     3961 aggcgggcgt gttcaactgc ccgacgctcg acttttacgc cgttgcgctc ggccctcgcg
     4021 atcacctggc cgagcgcgcg gggatcagcg gctacacgac ggccgatgag ctcgccacct
     4081 ggacccgggc gctcgccgaa cgagcggtgc cggccgatgc cgcggggcgc ctcgcgaaga
     4141 tcgccgccgt ggtcgtggcg ttgcgcgatg cgggtgcgcc gatcctcgtc ggcagcgaca
     4201 cgccggacac gttcgcgctg cccgggttcg gctacgtcga ggagctgcat gcgctggccc
     4261 gggtcgggct gtctccggcc gacgtcctgc gcgcggcgac cgcgagcgcg gcggcggcgc
     4321 tcgggcggcc cgatgacggc gcggtcgcag gcgcgcgggc cgatctcgtc ttgctcgagc
     4381 gggatccgct cgcgagcgtg gagaatctcg ctcacccggc cggggtcatg gtccggggac
     4441 ggtggtcgac gcgcgccgac ctcgacgcgt tgctcgctgc gcaccgcacc agcgccgcgg
     4501 cgcccgagta gcgaccgcac atcgtcgccg agcggtcgag gattgcaggt gtgggtcgac
     4561 ctcctaccac ggcactcgat caggctcggc gctccccatc tggaattacc tggtcgataa
     4621 cgtgttagcg tccttacctg tcgcagtatc agattgtgct catgtccttg cgacaccggg
     4681 aacagggtgt tctcatgcga aaacttccga ccggtgaatg caggcgcgtg gacgcacgcg
     4741 ctatcgtgtt caccgataca actacgccac aggaggcaag caccatgaag aaattctcat
     4801 tcaagtcggc gccccggaca ctgacggccg agaagcgcgc catgcgtgag cagatgctgc
     4861 gggccgtgcc cgaggagttg ctgtcgcaga tccgcggcgg cggactggag gacggctgcc
     4921 attcggcctt ctgctgctgt gatgacggct gctcgctgag catcctccag ggttagcgta
     4981 tcgaccccgt cctggcttcc ggtgaacgga tcaggggcag aatccatgag cgtcgatgcg
     5041 ttcatgggct ggctcgcgga tgcgacccgc gatgggcaag ctcatctcgc agacacgctg
     5101 cgggcgctgg tctatcgagc cgacccggag ctgttcgacg tgctcgagga cgtggaccag
     5161 gacgtgttcc tcgagccatt gctgttcgct cacttcgcgg tgaaccagcc gagcatcccg
     5221 ctgccccaga tcgtcgttgg ctacgcggac gcggtgatgc gcccggaggc ggtcgaggtt
     5281 cgctcggacg ccgcggggac gatctacctg ccgcgcatcg gctatttcct caccgaggcg
     5341 cgtgaccgga cgctcgtcct gcactgggac gcggagaccg ggcgcgcctc ggtgctcgac
     5401 ggcgacacgc cgatcgactt ccgtttcgag gagctgccca cgatcccggg cacggacatc
     5461 gaggtctgtc gccatggcct gcccatgctc gcggacgtgg tcgcggcggc gacgacgcac
     5521 ggcccggagt tctgggtggt cgacgatcgc cccgacggac atctcgacca cctggccacc
     5581 gcgatggaga tcatccgcgc acaatggccc acgtatcacg cgcagatcct gtccacggtc
     5641 cggcaggtgg tgctgttccg tagcgcgacg ctgaactcgt ttgcctcggt gtccgcgcat
     5701 ggcgtcgcat gcctgaacgt gagcgcgcgc gatgacgagg tgttcttcgt cgaggatctg
     5761 ttgcatcaat gcggtcacgt gatgttcaac gcggccacgg cgcgccgcaa ggagtacgtg
     5821 gccgtcgacc ctgacctgcc gatcggaccg ttcaacggac acccggacga cagccgctcg
     5881 gtctacgtgg tgctccacgg catgatgacg gagttcgcca tggtgcacgg tctgcggctg
     5941 tgcgacgagc gcggcacgtt cgggggacgc aaggcgcacg agttgaccgg ccggctggcg
     6001 ttcatcacgc acaaggcgct gagcgacatg cccagcctcg cgaacgcatc gctcctcacc
     6061 gcgaagggcc gctcgctgta cgagacgttt gccgccgcct tcgagggcat ccggcgcgag
     6121 cggtccgatc tgctgaccgg ctacacgatg gcgaaccagc cctacagctt cagctacgac
     6181 gtgttcacgc gcctgaaccc gatgccctga tggctcggtt ggatcagcgc cctttgaacc
     6241 tgaactcgac gccaggcgcg accgtgggac tgggtttggc tgcttcctgg agcctggtag
     6301 aagctctgag ggccgacgcg cacgggcccc ggacagcccc acccctgtcg cccgggccga
     6361 tcccgtccgt atgcccggta gggacggcac ctcgcgggtc cggccagtcg atctggtccg
     6421 cgcatacgta gcctcgccgg tgacgctacg cggcgcgacg cgacggctca cacacctctg
     6481 ccgcaaggac actccggatc tacgaccgaa cgtggcggta tcacgcttgc tccagtccga
     6541 agtccctgat atcttgcgca agttcgagtg gcgcttgcag cgttcgacct ctcgtcgagg
     6601 tcatcagaac gtcgaccact cacaaccaag aaagtagaga accatgaaga agaagaatga
     6661 caagaagtcg cttccgaaat tcaaggccgt cacgctcggc gcggacgagc tgagcagggc
     6721 ggtcggcggg ctggattgca cctgcggcac tgtgtccgtc tgccatgtcg atggcacgga
     6781 cgacggcgac ggcggcctcg agatatagac gacggcgacg gccagtcgta tgcactgact
     6841 gaccaggcag acgccgtcca ccggctccgg gggctggaga tcctctcgcc cccggtgcca
     6901 accacaccga cgagctccct catgatcctg atcttcagca ccgccgggga ttcatcgacc
     6961 ctcgacgtga tgagatggat aaagcatctg agcaacgcca aggtggtgcg aatcaacgcg
     7021 gacgaaacgt acagcgtgga cctgacgttc tccgaggacg attatcatat ccgtgtcgac
     7081 gacgatgcgt tctgcctcgc ggacgtgtcg agcgcatggc tccggaaggg agacttctgg
     7141 ttcagggacc tgtttcaccc cgtggtggcg ccttggtcct cggctctcag cagtcatttg
     7201 agccgcaagc tccagcggga agaccgtaac cttcgagatc attttcacta cgtgctgcag
     7261 aaacgctgcg ccgtgctggg aagcgcaaaa gggtcgagcc cgaacaagct ggtcgtcctg
     7321 gagcatgccc gtgaggttgg ccttcgaacg ccgccgttca gcgtctcgac ttcgcgccag
     7381 cacgcgcagc gcctgctgga gtccgattgc acgtatgtct ccaaggcgat gtcggacggc
     7441 ctgtatctgt tcgacacgag cgagagcaag gctgggtact tcacgtacac ggaagcgctc
     7501 gagcctcaca gcctggtggg ggtcggcgag cggatggcgc cgtcgttcct tcagcgctat
     7561 atcgagaaga aactcgagct gaggatattc ttcctggatc gtatgttcct cgcagtcgcc
     7621 atcttctccc aggccgacga tcagacgagg gtcgactacc gaaggtacaa catggagaag
     7681 ccgaatcgag tgatcccttt tcgactcccc caggagatag cgcacaagct gtcacttctc
     7741 agcgacaggc ttggcctgaa tactggctcc atcgatatga tcgtcgacaa ggaagatcag
     7801 ttttactttc tcgaagtcaa ccctgtcggg cagttcgccg gtcactcgga aacttgcaac
     7861 ctgtcgatcg aacgactgat cgcggaaagg ctcctcgcgt atgagcagtg atctgagacc
     7921 gctgagcttg cctcccctca tgacggagag gctcccgctc ggataccgca ggatcctcgt
     7981 cagcgacgac attctggacg acgaggctac cgagcggctg acgttcgagc attcgaagta
     8041 tcgtcatgtt tcgacgcacg tccgtcgtcc gttgaaaccg atctccaagg tggagttgta
     8101 gtcaccatgg acatggagaa ggtcctggca gactcccttg gaaagcacgt cctgctgttc
     8161 acggactgcg ttccggtcga gggccacacc gcctccgcga tctacgatct cacgcggggt
     8221 acaatctcga cgtttccgtc ggagtacttt ccgctcttca agctgtttcg gacgcatcgg
     8281 ctcggtcaac tcctcgatac gttcgaggag gacgatcggg ccaatcttca tgatttcctc
     8341 ggcttcttgc tcagcaacga gtacgtggcc ttcgtcgatg atctggcgcc gttcccggag
     8401 atcccttctg cctgggatgc accttgctcg atcgagaacg ccatcatcga cgtcaacacg
     8461 cagcgccatg actaccgaaa gatcgtctcg gagctcgacg ctcttggatg cctgcatctt
     8521 caggtccgga gctattcgcc cgtcttcacc gtctcggacc tggtcgatct cgctcgtctg
     8581 tgccgcggca cctcgatcca gacgctacaa gcggtcatcc cgcacgatcc gacgacttcc
     8641 gacgacgact acgtctcggt ggtgacctcc aaccgtatcc tcgtcggtct ggtcgtgcat
     8701 tcggccagcc aggatcgacg gatccgggtc gactatggcg cgcggggagc ctcggccaag
     8761 cttgtcgcct tggagatcga gatgaccacc aagaagctgg agtccgacct cgactgcggc
     8821 tcgatcacga agaagcagct ccttcgacca tcgacaccga cgttcaacga gcttcaccat
     8881 ttcaacggat gcttgaaccg gaagatctcg atcgacgaga acgggcaggt gcggaattgc
     8941 cctgcgatgg gaagatcatt tggccaccac c
//
LOCUS       DASVOR010000171         5520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_206234,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000171 DASVOR010000000
VERSION     DASVOR010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206234"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /gene="pilB"
                     /locus_tag="VF516_03855"
     CDS             complement(<1..1460)
                     /gene="pilB"
                     /locus_tag="VF516_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEX8106834.1"
                     /translation="MSRIGELLVREKMLSLQQLQQAQEEAKRTGKRLGATLSRLGYVK
                     DQDLTQFVARQYSLPSINLGEIEIEQAVLKLVPREICEKHQVIPVRRSGPTLVVAMAD
                     PSNIYAIDELKFLTQYNIEPVVASEGALETALSRYYDKGPDLDLIVGEFDVDNVDFSG
                     TADEAINIVDLENQAGEAPVVKLCNAILLSAIKKKASDIHVEPYEKSFRVRFRIDGIL
                     QEEMRPPLRLRNAITSRLKIMASLDIAERRLPQDGRIKLKIGNNREMDFRVSVLPTLF
                     GEKIVLRLLDKSTLQLDMTKLGFDPRPLEDFKVAIKKPYGMVLVTGPTGSGKTTTLYS
                     ALSELNTIDRNISTAEDPVEFNLHGINQVQMHEDIGLNFATALRSFLRQDPNIIMVGE
                     IRDFETAEIGVKAALTGHLVLSTLHTNDAPSTVSRLLNMGIEPFLVTASVNLVLAQRL
                     ARRICADCKIPDEKHPEALAKMGMTEDQIRKVTIMKG"
     gene            1683..1862
                     /locus_tag="VF516_03860"
     CDS             1683..1862
                     /locus_tag="VF516_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106835.1"
                     /translation="MSYAEAKEIALARIELRESFRIGDRAAAGAALARLVEVAHDDDE
                     LSAEIRRWKIKLDAV"
     gene            complement(1970..4006)
                     /locus_tag="VF516_03865"
     CDS             complement(1970..4006)
                     /locus_tag="VF516_03865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX8106836.1"
                     /translation="MKRIDFCLLGCWLGTAALPLAACTQAAGPDGVFEAAGGAISDNT
                     PGFIKHAKDLGPVDPSTVIEVTAWLKLHNEAQLDQLVAQQQTKGHANFHKWITQGQFN
                     AQFAPTAQEVNAVQNWLNAHNLTTEAVAENNFFVKVQGTVGDVEKAFHVQIDQFDLNG
                     QILRANVSNPSINDSSGNHVAAITGLDDLGFQPLNVRPVGPDGVAPAPVPLASVTPDG
                     GFFEGACFRANETHTFTGGGATATYTGTRYGSDITNTTLGHLAPCGYSPADVRAAYGM
                     NALYAAGLDGTGQTIVITDGFGSPTIERDAAVFSQFYGLPPVDLTIVKAPGLDHNPGS
                     AVWAGEVTLDVEWAHAMAPGAKIALVVAPARSSLDEAINYAVVHHLGNTISNSWSTLE
                     GFGNPAQLIRVERILQMAAAQGIDVNFSSGDNGDELGNVGFVTVDYPGSSPFATSIGG
                     TSLALDAQRHLVFETGWGTNETRIAGTTAEGNAPQSPPLDLGFVFGAGGGTSLTFAKP
                     GFQAALPGSKRMVPDIGMLADPFTGVEIIETDTATGQLGVSVIGGTSLACPLFSGVMA
                     IASQKAGTGLGQAAPLLYGLGSGIRDIASASVNNVTGQITPAAGTTALSASALAHPQT
                     SAPFVSAMYNSPFSTRWFVLTFNTDSSLAAAPGWDNVTGLGAPDGAAFVNAIAP"
     gene            4388..>5520
                     /locus_tag="VF516_03870"
     CDS             4388..>5520
                     /locus_tag="VF516_03870"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEX8106837.1"
                     /translation="MAVPRRPGRRPARGLPIVRKNTVRLPGDVAHRIRAGHPWVYREA
                     LAPRPLVAEPGTVIDCVDEDGEFVGRGLYDGDSAIALRVFVRKPDVAIDRRLIGERVR
                     AAIALRRKLLDLDRLGATRMINAESDGLPGIVVERYGDYLVTQLFTGAVSGLRDDLYD
                     ALEAELGPKAIYEQRRFKSLGGEAPRQAAAELVRGSAAPVELEVKEDDLTFVVDVTAP
                     LSTGLFADLREGRRAVRHWAAGRRVLNLFSYTGAISVYAQAGGASEVCAVDVAAQAHA
                     RARRNFAASGFDPEKPEHIVGDAFKVLARFVERRRAFDLVVLDPPAFASAAARGGKPW
                     SAVRDFSELVTASLEVLVPGGLLIAASSTHKMSAAEFELALADG"
BASE COUNT          791 a         1878 c         1957 g          894 t
ORIGIN      
        1 cccttcatga tcgtgacctt gcggatctgg tcctcggtca tccccatctt ggcgagcgcc
       61 tcggggtgct tctcgtcggg gatcttgcag tcggcgcaga tcctgcgggc cagccgctgg
      121 gcgagcacga ggttgaccga ggcggtgacc aggaacggct cgatccccat gttgagcagg
      181 cgcgacacgg tcgacggcgc gtcgttggtg tgcagggtcg acagcacgag gtggccggtc
      241 agcgcggcct tgaccccgat ctcggcggtc tcgaagtcgc ggatctcgcc gaccatgatg
      301 atgttcgggt cctggcgcag gaacgatcgc agcgcggtcg cgaagttgag cccgatgtcc
      361 tcgtgcatct gcacctggtt gatcccgtgc agattgaact cgaccggatc ctccgccgtc
      421 gagatgttgc gatcgatggt gttgagctcg gacagcgccg agtacagcgt ggtggtcttc
      481 ccggagccgg tcggaccggt gaccagcacc atcccgtagg gcttcttgat cgcgaccttg
      541 aagtcctcga gcggccgcgg atcgaacccg agcttggtca tgtcgagctg cagggtggac
      601 ttgtccagca gccgcaggac gatcttctcg ccgaacaggg tcggcaggac cgacacgcgg
      661 aagtccatct cccggttgtt gccgatcttg agcttgatcc ggccgtcctg gggcaagcgg
      721 cgctcggcga tgtcgagcga ggccatgatc ttgagccgcg aggtgatcgc gttgcgcagc
      781 ctgagcggcg gccgcatctc ctcctggagg atgccgtcga tccggaaccg gacccggaag
      841 ctcttctcgt agggctcgac gtggatgtcc gacgccttct tcttgatcgc cgacagcagg
      901 atcgcgttgc acagcttgac caccggcgcc tcgccggcct ggttctcgag gtcgacgatg
      961 ttgatcgcct cgtccgccgt gcccgagaag tcgacgttgt cgacgtcgaa ctcccccacg
     1021 atcagatcga gatcggggcc cttgtcgtag tagcgcgaca gcgcggtctc gagcgcgccc
     1081 tccgacgcga ccaccggctc gatgttgtac tgggtgagga acttgagctc gtcgatcgcg
     1141 tagatgttcg acgggtcggc catcgcgacg accagcgtcg ggcccgaccg ccgcaccggg
     1201 atcacctggt gcttctcgca gatctcgcgc ggcaccagct tgagcaccgc ctgctcgatc
     1261 tcgatctcgc cgaggttgat cgacggcagg ctgtactgtc tggcgacgaa ctgcgtcagg
     1321 tcctggtcct tgacgtagcc gagccgcgac agcgtcgcgc cgagccgctt gccggtccgc
     1381 ttggcctctt cctgcgcctg ctgcagctgc tggagcgaga gcatcttctc gcgtaccagc
     1441 agctctccga tccggctcat tgcggcggtg tctcctgaaa agcctgcggt tttcacaggc
     1501 taccgcgacg gtcttggaga cgtcaaaccc cgctccccca gggttacccg acgcgtaccg
     1561 acgtgtaggt gtcgggcaag gtcagagcca tcctcgaata tgccgccgtc cggttcgtcc
     1621 gagaagttgc cggcgcctcg aaccgcgcgg aacgtggacg actggataga caggaccgat
     1681 cgatgagcta cgcagaggcc aaggagatcg cgctcgcgcg gatcgagctg cgcgagagct
     1741 tccgcatcgg cgaccgggcg gcggccggcg cggcgctcgc ccggctggtc gaggtcgcgc
     1801 acgacgacga cgagctgtcg gccgagatcc ggcgctggaa gatcaagctc gacgccgtct
     1861 gaccgccgcc tgtctcccgg ttccggccgg ttccggccgc ctggaaagcg gccacgagga
     1921 tccgggagac agcgcggcgc cggtcacacg gcccggccgc cggacgagat cacggcgcga
     1981 tggcgttgac gaacgcggcg ccgtccgggg cgcccaggcc ggtgacgttg tcccagccgg
     2041 gcgcggcggc gagcgagctg tcggtgttga acgtcagcac gaaccaccgg gtcgagaacg
     2101 ggctgttgta catcgcgctg acgaacgggg cggaggtctg tgggtgcgcc agcgcgctgg
     2161 ccgacagcgc ggtcgtaccg gcggcggggg tgatctggcc ggtcacgttg ttgaccgacg
     2221 cggacgcgat gtcgcggatg cccgagccga ggccgtacag cagcggcgcc gcctggccaa
     2281 gcccggtgcc cgccttctgc gacgcgatcg ccatcacccc ggagaacagc gggcaggcca
     2341 ggctggtgcc gccgatcacc gagacgccga gctgaccggt cgcggtgtcg gtctcgatga
     2401 tctcgacgcc ggtgaacgga tcggccagca tgccgatgtc ggggaccatg cgcttgctcc
     2461 ccggcagcgc ggcctggaag cccggcttgg cgaacgtcag gctggtgccg ccgccggccc
     2521 cgaacacgaa gccgaggtcg agcggcgggc tctgcggcgc gttgccctcg gcggtggtgc
     2581 cggcgatccg ggtctcgttg gtgccccacc cggtctcgaa caccaggtgg cgctgcgcgt
     2641 cgagcgcgag gctggtgccg ccgatgctgg tcgcgaacgg cgagctgccc gggtaatcga
     2701 cggtgacgaa cccgacgttg ccgagctcgt cgccgttgtc gccggacgag aagttgacgt
     2761 cgatgccctg cgcggcggcc atctgcagga tgcgctcgac ccggatcagc tgcgccgggt
     2821 tgccgaagcc ctccagcgtc gaccagctgt tcgagatcgt gttgccgagg tgatgcacga
     2881 cggcgtagtt gatcgcctcg tcgagcgacg atcgcgcggg cgcgaccacc agcgcgatct
     2941 tggcgcccgg cgccatcgcg tgcgcccact cgacgtcgag cgtgacctcg cccgcccaga
     3001 ccgcgctgcc cgggttgtgg tccaggccgg gggccttgac gatcgtgaga tcgaccggcg
     3061 gcaagccgta gaactgcgag aacaccgcgg catcgcgctc gatggtcggc gacccgaacc
     3121 cgtcggtgat cacgatggtc tggccggtcc cgtcgaggcc ggccgcgtac agcgcgttca
     3181 tgccgtacgc ggcgcggacg tcggccgggc tatagccgca cggcgcgagg tggccgagcg
     3241 tggtgttggt gatgtccgag ccgtagcgcg tgccggtgta ggtcgcggtc gcgccgccgc
     3301 cggtgaacgt gtgggtctcg ttggcgcgga agcacgcgcc ctcgaagaac ccgccatcgg
     3361 gggtcacgct ggccagcggg acgggcgccg gggcgacgcc gtcgggaccg accggccgca
     3421 cgttcagggg ctggaagccg agatcgtcga gcccggtgat cgcggcgacg tggttgcccg
     3481 agctgtcgtt gatcgacggg ttcgacacgt tggcgcgcag gatctggccg ttgagatcga
     3541 actgatcgat ctggacgtgg aacgccttct cgacgtcacc caccgtgccc tggaccttga
     3601 cgaagaagtt gttctcggcg accgcctccg tggtgaggtt gtgggcgttc agccagttct
     3661 gcaccgcgtt gacctcctgc gcggtcggcg cgaactgggc gttgaactgg ccctgggtga
     3721 tccacttgtg gaagttggcg tggcccttgg tctgctgctg ggcgaccagc tggtcgagct
     3781 gggcctcgtt gtgcagcttg agccacgcgg tcacctcgat caccgtcgac ggatcgaccg
     3841 gcccgaggtc ctttgcgtgc ttgatgaagc cgggtgtgtt gtccgagatc gcgcctcccg
     3901 cggcctcgaa caccccgtcg gggccggccg cctgggtaca tgccgccagc ggcagcgccg
     3961 cggtcccgag ccagcacccg agcagacaaa agtcgatgcg tttcacgctg tacctcgctg
     4021 aatgtttgta tccgcgaata gcaagtatcg cggtcatggt caatcagcga ccgcgcgcag
     4081 gctcgacgga agcgacccag attacagatc agcgaatcac ggagagtgac tcctatctat
     4141 cgctgcggcg tgtcgccagc tgcggtgtcg atcgtgtcag cggcggcgaa cgaaaaaccc
     4201 gcaaccgccc ggcccgccga tcccacagcc ggtgatgagc ggatccccgg gtgcgccgca
     4261 gccacggcgc ggcccgccat gtcaaggact tggcgcggcc ggcggcccga ccccgcccct
     4321 ggcgccgggc caattgccgc cggtccggct tggagattcc cggcacttgc gccataaagc
     4381 cggcgctatg gcagtgcccc gccgccccgg tcgccgcccc gcccgcggcc tgccgatcgt
     4441 ccgcaagaac accgtccggc tgccgggcga cgtcgcccat cgcatccggg ccgggcaccc
     4501 ctgggtgtac cgcgaggcgc tcgcgccgcg gccgctggtc gccgagccgg gcacggtgat
     4561 cgactgcgtc gacgaggacg gcgagttcgt cggccgcggc ctgtacgacg gcgacagcgc
     4621 gatcgcgctc cgcgtgttcg tgcgcaagcc cgacgtcgcg atcgaccgcc ggctgatcgg
     4681 cgagcgggtg cgcgccgcga tcgcgctgcg ccgcaagctg ctcgacctcg accggctcgg
     4741 cgcgacccgg atgatcaacg ccgagtccga cggcctgccc ggcatcgtgg tcgagcgcta
     4801 cggcgactac ctggtgaccc agctgttcac cggcgcggtc agcggcctgc gcgacgacct
     4861 gtacgacgcg ctggaggccg agctcggccc caaggcgatc tacgagcagc gccggttcaa
     4921 gtcgctcggc ggcgaggcgc cccgccaggc cgccgccgag ctggtccgcg gcagcgcggc
     4981 gccggtcgag ctcgaggtca aggaagacga tctgacgttc gtcgtcgacg tcaccgcgcc
     5041 gctgtcgacc gggctgttcg ccgacctgcg cgagggccgg cgcgcggtcc gccactgggc
     5101 ggcgggccgc cgggtgctca acctgttctc gtacaccggc gcgatctcgg tctacgccca
     5161 ggccggcggt gccagcgagg tctgcgcggt cgacgtcgcc gcccaggccc acgcgcgggc
     5221 gcgccgcaac ttcgccgcct ccggcttcga tcccgagaag ccggagcaca tcgtcggcga
     5281 tgcgttcaag gtgctggcgc ggttcgtcga gcgccgccgg gcgttcgacc tggtcgtgct
     5341 cgatccaccg gcgttcgcga gcgccgctgc gcgcggcggc aagccgtgga gcgcagtgcg
     5401 cgatttctcc gagctggtca ccgccagcct cgaggtcctg gtgccgggcg gcctgctcat
     5461 cgccgcatcg tcgacgcaca agatgtcggc cgccgagttc gagctcgccc tcgccgacgg
//
LOCUS       DASVOR010000172         4303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439963,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000172 DASVOR010000000
VERSION     DASVOR010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4303
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439963"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VF516_03875"
     CDS             complement(<1..960)
                     /locus_tag="VF516_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX8106838.1"
                     /translation="MTSSTTTLVSKPHARIDRGTTWTLCVALDCEHVNATPVRVSLAD
                     LTEVELGRSTARAFTRTRTRLRVDLANRGASQLHARMTAHADRWKIEDAGSKNGTLVN
                     GKRAKAAMLDDGDVIECGGTFLVIRQARGPVGGIEEPEARLGALRTLSPALQRELAIV
                     PKLARSKLPMLIRGESGTGKEVAAHTIHELSGRRGAMIAVNCGAIPATLIESELFGSR
                     RGAFSGAEDREGIVRGAEHGTLFLDEVAELPLPSQVALLRLLQNGEVLSLGASKVAAV
                     DVRVVAATHQPMEMLVESGRFRRDLYARLRGHELRLLPLAQRLE"
     gene            1061..4039
                     /locus_tag="VF516_03880"
     CDS             1061..4039
                     /locus_tag="VF516_03880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106839.1"
                     /translation="MGERTATQEHGELDSDVGHPSGLQSSAHGDRDDAPRSAANPAAV
                     VTVGSSLGRFTVTSSIGGGGMGEVFAAHDQELGRTVALKVIRPDRLAHSPTARLRLMR
                     EAQALARLHHPNVVTVYEIGTEGEHVFLAMELIEGEPLSAWLRKATHAWREVLARFLA
                     AGRGLAAAHEAGIIHRDFKPDNVVVGEDRVVVVDFGLARGGEDDGGENRESAPSLLET
                     SLTETGDRCGTLPYMAPEQLSGSRAVPQSDQYAFCVSLWEGLHGERPTDTALRPRNTE
                     VPDWANEALRRGLSTDPAIRWPSMQALLTELGRDRRAARNRALLVAGAAVLVATASWG
                     LLRPAALDPCAGGATRVAQAWGPARQQAVHAAFLATKAPGAEDTFHRTRAVLDDYATR
                     WAAMYGEACRATRVEGRQSDSMMDLRMECLERRRAVLGNLTALWGRGVDADTLEHAAD
                     AVSNLGSLRDCADTRVLGERTPVPTDPRVTARVNEVRRRLDEVQAQIAAHRLSEGRKA
                     AGEVRALAENIDWPPLAAEASFAEGDILAALQDPSAEAPLMTASRLATAGHDDRLAAR
                     ALIRLVDVLGAVKQNAARALLVADLTEGQVTRVGDDQLTAKLLYARGNAQLVDGKIDD
                     AGSTLQRAHDLSVAAFGAGDRETLLTMMALAEVARQKGDYELACKLGEQTLAATIERL
                     GPEHPEVIRALETLGSSSNDAADRVGAAAYFQRALALSEKIAGLESAQTANELFKLGT
                     SLQSIGKIEEAERVLERALAIRQKVLPPEDPNLGATLGVLGSLRAQQGRFDEAVLLQQ
                     RTVSIFTKAYGPNHPKLAIALHKLGATYEMKGDPTTALAYFQKALDLRIQILGPDHEM
                     TLFSTTLVGEALVDLHRCREARPLLEKTAGALAKKVGESHPDFLRTINAVAECDLAEG
                     HPAQAVARLEHAVELEAGANHSTAARGGHRFLLARALWGVGRRREAVLAARQAMDELA
                     TEPSGAPLAADIQRWLETHR"
     gene            complement(4055..>4303)
                     /locus_tag="VF516_03885"
     CDS             complement(4055..>4303)
                     /locus_tag="VF516_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8106840.1"
                     /translation="AMHRLRPGSQRPAAALALGLGAAAFVATSRVLAGQHFITDALGG
                     ALVGSSVGVAVSSLHGSPISVVPVMGEHQQGLALRTAF"
BASE COUNT          678 a         1539 c         1502 g          584 t
ORIGIN      
        1 ctcgagccgc tgcgcgagcg gcagcaggcg cagctcgtgg ccgcgcagcc gcgcgtagag
       61 gtcgcggcga aaccggccgc tctcgaccag catctccatg ggctgatgcg tcgccgcgac
      121 gacccggaca tcgaccgcgg ccaccttgct cgctccgagc gacaggacct cgccgttctg
      181 caacagccgc agcaaggcga cctgcgacgg caacggcagc tcggcgacct cgtcgagaaa
      241 cagcgtgcca tgctcggcgc cgcggacgat cccttcgcgg tcctcggcac cggagaacgc
      301 accacgtcgg ctgccgaaca gctcgctctc gatcagcgtg gccgggatcg caccgcagtt
      361 gaccgcgatc atcgcgccgc ggcgtcccga cagctcgtgg atcgtgtggg ccgcgacctc
      421 cttgccggtc cccgattcgc cgcggatgag catcggcagc ttcgaacgcg ccagcttggg
      481 cacgatcgcg agctcgcgct gcaacgccgg cgacaacgtg cgcagcgcgc cgagccgcgc
      541 ctcgggttcc tcgataccgc cgactggccc ccgcgcctgg cggattacca ggaacgtacc
      601 gccgcactcg atgacgtcgc cgtcgtccag catcgcggcc ttcgcacgct tgccgttgac
      661 gagcgtgccg ttcttcgaac cggcatcctc gatcttccag cgatccgcgt gcgcggtcat
      721 ccgcgcatgg agctgcgaag cgccgcggtt cgcgaggtcg acgcgcagcc gtgtcctcgt
      781 ccgcgtgaac gcgcgcgcgg tggaccggcc gagctcgacc tcggtgaggt cggcgagcga
      841 gacccggacc ggcgtggcgt tcacgtgctc gcagtcgagg gcaacgcaca gcgtccacgt
      901 ggtcccgcgg tcgatacgcg cgtgaggctt cgacaccagc gttgtcgtcg agctcgtcac
      961 gcgcctagca tagacattct ccaggaggta ggctattctt gttcctgccc cctcgatatg
     1021 cagtaccaat gctgagcggt gtacgttgag gatcgcgggg atgggggaac gcaccgcaac
     1081 ccaggagcac ggagagctcg actccgacgt cggccaccca tccgggctgc agtcgagcgc
     1141 gcacggcgac cgagacgacg cgccgcggtc ggccgcgaac cccgcggcgg tggtcaccgt
     1201 aggcagctcc ctggggcgct tcaccgtcac ctcatcgatc ggcggcgggg ggatgggcga
     1261 ggtgttcgcc gcgcacgacc aggagctcgg gcgcacggtc gcgctcaagg tgatccggcc
     1321 cgatcggctg gcgcactccc cgaccgctcg cctgcgcctg atgcgcgaag cccaggccct
     1381 cgcccgactc caccacccga acgtggtcac cgtctacgag atcggcaccg aaggcgagca
     1441 cgtgttcttg gcgatggagt tgatcgaagg cgagccgctg agcgcctggc tccgcaaagc
     1501 aacgcatgcc tggcgcgaag tcctcgctcg cttcctcgcc gccggccggg gcctcgcagc
     1561 ggcgcacgag gccgggatca ttcaccgcga cttcaagccc gacaacgtgg tcgtcggtga
     1621 ggatcgggtc gtcgtcgtcg acttcgggct cgctcgcgga ggggaggacg acggcggcga
     1681 gaaccgcgaa tccgcgccca gcctgctcga gaccagcctc accgagaccg gcgatcgctg
     1741 cggcaccttg ccctacatgg ctccggagca gctgagcgga tcccgcgccg ttcctcagtc
     1801 ggaccagtac gcgttctgcg tgtcgctctg ggaagggctc cacggcgaac gccccaccga
     1861 tacggctctc aggccgcgca acaccgaggt gcctgactgg gcaaacgagg ccctccgccg
     1921 cgggctgtcc accgatcccg ccatccgctg gccgtcgatg caagcgctct tgaccgagct
     1981 cgggcgtgac cggagagcgg ccaggaaccg cgcgctgctc gtcgccggtg cggcggtgct
     2041 cgtcgccact gcgagctggg gcctgcttcg cccggccgcg ctcgatccct gcgcgggcgg
     2101 cgcgacccgc gtggcgcagg cgtggggacc tgcccggcag caagcggtgc acgcggcgtt
     2161 cctcgcgacg aaggcacctg gcgcagaaga caccttccac cgcacgcgcg cggtcctcga
     2221 cgactacgcc acccggtggg ctgcgatgta cggcgaagct tgccgcgcga cccgggtgga
     2281 agggcgccag tcggactcga tgatggacct gcggatggag tgcctcgagc gacggcgcgc
     2341 ggtgctgggg aatctcaccg cgctatgggg tcgtggagtc gacgccgata cgctggagca
     2401 tgcggcggac gcggtgagca acctcggttc gctccgcgac tgcgccgaca cgcgcgtgct
     2461 cggcgagcgc acgccggttc cgaccgatcc tcgggtcacg gcccgcgtca acgaggtgcg
     2521 gcggcgactc gacgaggttc aggcccagat cgctgcccat cgcctgtcgg aggggcgcaa
     2581 ggccgccggc gaggtgcgtg ccctcgccga gaacattgac tggccgccgc tggccgcgga
     2641 ggcgagcttc gccgaaggcg acatcctggc cgccctccag gatccgagcg cggaggcgcc
     2701 gctcatgacc gcatcccgcc tggccaccgc cggccatgac gaccggctcg ctgcgcgggc
     2761 gctgatccgg ctggtcgacg tgctcggcgc tgtgaagcag aacgccgcac gggcgctcct
     2821 ggtcgccgat ctgaccgaag ggcaagtgac ccgggtaggc gacgatcagc tgacggccaa
     2881 gctgctgtac gcccgcggca acgcccagct cgtagacgga aagatcgacg acgccgggtc
     2941 gaccttgcag cgcgcccacg acctctcggt cgcagcgttc ggcgccgggg accgggagac
     3001 gttgctgacc atgatggcgc tcgccgaggt cgcacgccag aagggcgatt acgagcttgc
     3061 ctgcaagctc ggtgagcaga cgctcgcggc caccatcgag cggctcgggc ccgagcaccc
     3121 cgaggtcata cgagcgctcg agacgctcgg gagctcgtcg aacgacgccg ccgaccgcgt
     3181 gggcgccgcg gcgtacttcc agcgtgcgct cgcgctcagc gagaagatcg cggggctgga
     3241 gtcggcgcag acggccaatg agctgttcaa gctcggaacg tcgcttcaga gcatcggcaa
     3301 gattgaagag gccgagcggg ttctcgagcg cgcgctggcg attcgacaga aggtgctgcc
     3361 ccccgaagat ccgaacctcg gagctaccct gggcgtgctg ggatcgctcc gagcccagca
     3421 gggtcgcttc gacgaggccg tcctgctcca gcagcgcacc gtgtccatct tcaccaaggc
     3481 atacggcccg aaccacccga agctcgccat cgccctccac aagctgggcg ccacctatga
     3541 gatgaagggg gaccccacga ccgctctcgc gtatttccag aaggcgctgg acctgcgcat
     3601 ccagatcctc ggaccggatc acgagatgac gctgttctcg accacgctgg tcggcgaggc
     3661 gctggtcgat ctgcaccgct gccgcgaggc gcgtcccctg ctcgaaaaga ccgccggtgc
     3721 gctggcaaag aaggtcgggg agagccaccc ggacttcctc cgcacgatca acgccgtggc
     3781 cgaatgcgac ctggcagaag ggcatccggc gcaggcggtc gcgcgactgg aacacgccgt
     3841 ggagctcgag gcgggggcga accacagcac tgcggcccgg ggcggccatc gtttcttgct
     3901 cgcacgcgcg ctgtggggag tcggccggcg ccgcgaggcg gtcctggccg cgcgacaggc
     3961 gatggacgag ctggcgaccg aaccctcggg agcaccgctc gcggccgaca tccagcgctg
     4021 gctcgaaacc catcgctgag gtcagccgcc gcgatcagaa cgcggtgcgc agggcgagtc
     4081 cctgctggtg ctcgcccatc accgggacga ccgagatcgg cgagccgtgg agcgacgaca
     4141 ccgcgacgcc gaccgacgag ccgaccagtg cgccgcccag cgcgtcggtg atgaagtgct
     4201 ggcccgccag cacgcgcgag gtcgcgacga acgccgccgc gcccaggccg agcgcgagcg
     4261 ccgcggcggg ccgctgcgag cccgggcgca gccggtgcat cgc
//
LOCUS       DASVOR010000173         9805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_235389,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000173 DASVOR010000000
VERSION     DASVOR010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9805
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235389"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(437..1747)
                     /locus_tag="VF516_03890"
     CDS             complement(437..1747)
                     /locus_tag="VF516_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106841.1"
                     /translation="MIDLGLQLRSACGRRRVDVRKYDARAFVRGSRRDLARGMRCRRC
                     RRDVDVDRSMRVMRCMRICDVRGMRFRNVRGVRRGGLEDFGSGSVRRMRFAKRSAGCV
                     ARVRFAKLSIRAVTCMRLAKLSAGCVARVRFAKLSICAVTCMRLPELTVTCVCDMRPT
                     KLTIRFVTGMRLAKLSIGSVTCMRLARLSLSSVTGMRLAKLSIGSVTCMRLAELVVAR
                     TSSVRLTLAISSVRRMRVARLSIGSARRTRITMLTMRSMSRGRLAIRAGRSVVRVRLP
                     GSPRLLCAADDRRCVVRAYCSARFALCARRTTDVRSAASLCTKRAPNVAEPEPRQGLR
                     LGGTCRRRGRSRRSYFRLDRSVASRRRALGRRIRNHLHERLLANQPGRRRERTTIIIA
                     IRSCPTRLLSHHSSIVANAVDNFYAERAAAGECSGVLRAISTCA"
     gene            1793..3955
                     /locus_tag="VF516_03895"
     CDS             1793..3955
                     /locus_tag="VF516_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106842.1"
                     /translation="MTAAGAAEKAKLGATFAKAHNDVAASAAQAHGQIAAQSTAQGAA
                     LTNWHTGAVAAANASFARGQTRARKAGDDGAQKATTAANRAAATIDSKLKDHSQQAHA
                     IGAQKAGAGGAPEVADAKAKAASKISGDAAQKIGDLGHVTGELRAMAPSTASGLRQKG
                     EEAARAIATGGPQVTAQYGAVHASTAQAMQALTGKGAQSLDQMKAQTAAGLHAHHQRA
                     ITGLDQHTAKSAAEIKAAGSHAGAAYRKQGVETMHASEQALGALKQKVAAQPIDPQRA
                     AAAGTQAKAEIQRGFDGIAGKAHAPVDQMTSHISAAGEHAVGGLGKVHGQIQPQVAGV
                     VDHARGSGSKLASGVGSKLAGAMTGARGAGSQMQAQVAAGIDNQVGKVDKAVDKGVGD
                     LQTGLDAKVSEADQKAQQPLNQVGPKIDQAQAKIEADAHKSTLDKVLSWVGDQLKQLG
                     RMLLDPGFWVGLLVAVVLIALLPEELAGLALICAMAAIGAVAAGIGTLVSNIAAGRPP
                     LQNVLQNMLVGALFGGALTFVGAALGTGLVGIAAMMGTAGVLTVIANLATGKPWDEGL
                     LANVLLVGVFAGIAKLFGRPGALRSGKDPKPGDPVTPSDPVEPGKGAGGNGTASEFGD
                     LSNMTRAEADTFLRSRGATVKQTAGGYTEYKFPDKSVVWIRPDGEIVRTPAPKYGPDG
                     RRINKGARLDQNGNPTDSHNTGERVKGDSPSDQPPEDK"
     gene            4089..4277
                     /locus_tag="VF516_03900"
     CDS             4089..4277
                     /locus_tag="VF516_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106843.1"
                     /translation="MHFQNCRRIQWDVHDAEGAEDESAELIGLLLGEGGHGAPAILTT
                     DIFELQILYGSFHLRKDW"
     gene            complement(4353..4982)
                     /locus_tag="VF516_03905"
     CDS             complement(4353..4982)
                     /locus_tag="VF516_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8106844.1"
                     /translation="MTAGARAARLTPAPGTVPTRDRIVFAATQLFQRRGYHAVSTADI
                     LEQARAPKGSMYHHFPLGKEQIAIAAVERIRGDVLALLRKLQADGRSLEDTLRVMAAG
                     MARWLRDSAWREGTMLASTAVGAVPDLPKLHAAIREAFDEWREHIAGRLVNDGWDTAS
                     AQALAQTLLAGIEGAMMLARVDQNERIVTCVVSMLARLISSGDGDRASP"
     gene            complement(4979..5401)
                     /locus_tag="VF516_03910"
     CDS             complement(4979..5401)
                     /locus_tag="VF516_03910"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8106845.1"
                     /translation="MVTIPAEFEPHFRKSPVTAPWEPLYSRRRADGVDLAFEVQPAHC
                     NSRGLLHGGVLAALCDNVMGLSLATALRAEANVVTISLALDYLDSARLGDLVIIVPRV
                     IRAGASLGFCDALATSGDRPIARANASFSVRIVDANRS"
     gene            complement(5453..6037)
                     /locus_tag="VF516_03915"
     CDS             complement(5453..6037)
                     /locus_tag="VF516_03915"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8106846.1"
                     /translation="MRKLIVHGFVTLDGILQAPGGPDEDRDGGFTHGGWSRPYWHDDI
                     GTKFGAIMQDADAFLLGRRTYVTHAQAFEPMPAGDPFGDMMNAPAKYVVSRTLDKPIW
                     RNTTIIRDDAVGAVRRLKAAAGKAILLDGSSQLLHTLFEHDLVDELYLDVYPLALGRG
                     KRVFPATGIDRRFALAKATPYPTGVVGLHYTRAA"
     gene            6209..6901
                     /locus_tag="VF516_03920"
     CDS             6209..6901
                     /locus_tag="VF516_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEX8106847.1"
                     /translation="MRYTALLLDLDHTLLDSDASERLAFEHALAGAGVADPAGYFPAY
                     DAINRALWARVERGEIHATQVRVQRFAQLIAATGIAASPDALADAYASGLCAHGELYP
                     GARDVLDALDAEPAVSLALVTNGLSEVQRARIDRLGLGRYFDVIAISAELGTAKPASA
                     IFEFALAGLGAPARAAALMVGDSLTSDIQGGRNAGIATCWYNPHRRPRDQDGLVDHEI
                     ATLDALLALVRS"
     gene            complement(6933..8021)
                     /locus_tag="VF516_03925"
     CDS             complement(6933..8021)
                     /locus_tag="VF516_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106848.1"
                     /translation="MRLVALVALSLAGCSLYFGSDAPEHGGPPDGGRPGGSPPDGGRP
                     GGLPDGGRPGGGACDGGSPVPPDAGGSPPGSCGVPELHVIAMYETSGNHSVTGDASVV
                     IERPGEHILVLSAYVATNWHVRLATGATVRAVELLGYDPQTVDLGSVPVTRGTACGYS
                     YPYNGGGCDTNQLLALAEAQAGVGLTTFHGCYQASSWTLHADGTATSNCNTSAGYQQY
                     DRFGTCLGWERLDFSTLSTPGCTGDRFVRHDDHYGVWVGAILCGSSKSYKLYMSATRD
                     EPFLEIADYAGYGQDHCELVDPAFTIPDEDDIRSGGCTDCALGQLVDPIGVPVYARAR
                     FGEPFERVISQYWADLTTTSYSCGVAIP"
     gene            8155..>9805
                     /locus_tag="VF516_03930"
     CDS             8155..>9805
                     /locus_tag="VF516_03930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106849.1"
                     /translation="MAPDLEYCRVLLRVILCRRTGDVIEGDGGMPRFESRSGRFVELE
                     IEDTVLTQVEGTVDGSYVGKPSTRTFSTADEARYAAARWIARRRKRDGYQLVGQARHL
                     GGGPGTIPRPPGPVSVIAARACWTAADPQFLSELLRPAAAAHAAGLAGLAERWFRDDR
                     PWARDMLRAYIDDGCDRPWHKALVKRLFKLAEAAGDDELMTHFLVAFDRLSRRYLIKE
                     SKYAGWDRAARRAIVLESMRLVQDPSIPARLPEREATAGGKGKAGARNQPGRKRPAEE
                     PQFTRVTRRYLARRAYRYFRMLAHRDLARYGRAMRLALPRYTDESLSTATRLLDAWGL
                     MHALYARSPAIVRSPAGIRLADGKTLADLAPAPHFDAAWDGVFSELLALASDAGSRTV
                     RGWAVALLRARYAGDLAALAFPAIKRLVMSPHDEVVALGIERLTRMSGLETVALHDWL
                     DLLAVQNLDVLPAIAELAERSLSPARLPLAQCIDLACGKTAPVARLGLVWAKTRAIQT
                     ADDLRAIARLARAGVATVRDEGTAWALEVIAAHPATRPDELR"
BASE COUNT         1611 a         3294 c         3512 g         1388 t
ORIGIN      
        1 gagggccacg gcgtggcacc cgatgacgag gtcagcgacg accgtgcggc gatcgcgacc
       61 gggcacgatc gcgcatgcga ggctgcgatc gcgtgggcgt gccagcgcca gccggcgccg
      121 tgcccgtgag caaccggcgc acagggcttc gccggccgcg tgatcacact cgctccgtac
      181 gtcgcgccga gccggccgtc acgcatgaag tcgcgcatca tcgcgaagta gccggctgcg
      241 tagcgcatcg atacgcgctg gccggtccgc gatcgctcgt acgcggtcat gccatgctcg
      301 gcgtgcgcga acgaggtcga ccgccgtcgc ccgcgccgta ggcgcggaca gcgtaccacc
      361 gccgccgtca aggacggggc tcgcgcatcg attacggatc gggacggacg tgacgtttac
      421 agccggaatg aatgaatcat gcgcacgttg aaatggcccg caacactccg ctgcattcgc
      481 cagcagcagc gcgctctgcg tagaagttgt caaccgcgtt cgctacgata gaactgtggt
      541 gggaaagcaa tcgggtcgga cagctgcgga tcgcaatgat gatcgtcgtt cgctcgcgac
      601 gtcgaccggg ttggttggca agcaaacgct cgtggagatg attccgaatc cgccggccaa
      661 gagcgcgcct gcggctggcg acgctgcggt caagtcggaa gtagctccgc cgagaacggc
      721 cgcgccttcg gcacgtgcct ccgagccgca acccttgccg cggctccggc tcggcgacgt
      781 ttggcgcacg cttggtgcag agactcgcag cgctacggac gtcagtggtg cgtcgagcgc
      841 agagagcaaa gcgcgcgctg cagtacgcac ggacgacgca tcgtcggtcg tcggccgcgc
      901 agagcaggcg ggggctgccg gggagccgca cgcggaccac ggaacggccg gcccgtatgg
      961 cgagccgccc acgactcatg gagcgcatgg tcagcatggt gatccgcgtg cggcgcgcgg
     1021 agccgatgga cagcctggcg acccgcatgc ggcgcacgga gctgatggca agagtgagcc
     1081 gcacgctgct cgtgcgagcg acgaccagct cggcgagtcg catgcaggtc acggagccga
     1141 tggacagctt ggcgagccgc atgccggtca cggaggagag ggacagcctg gcgagtcgca
     1201 tgcaggtcac ggagccgatg gacagcttgg cgagccgcat gccggtcacg aagcggatgg
     1261 tcagcttggt ggggcgcatg tcgcacacac aggtgacggt gagctcgggg agccgcatgc
     1321 aggtcacggc gcagatggac agcttggcga accgcacgcg ggccacacag ccggcggaca
     1381 gcttggcgag ccgcatgcag gtcacggcgc ggatggacag cttggcgaac cgcacgcggg
     1441 ccacacagcc ggcggaccgc ttggcgaacc gcatgcggcg cacgcttccg ctcccgaagt
     1501 cttccaggcc gccgcgccgc acaccccgca cgttgcggaa gcgcatccct cgcacatcgc
     1561 agatgcgcat gcagcgcatg acgcgcatgg accggtccac gtcgacgtcc cgccggcatc
     1621 gccggcaacg cattccgcga gcgaggtccc gtcgggatcc gcgcacgaag gcacgcgcgt
     1681 cgtacttgcg cacatcgacg cggcgacgac cgcaggcaga gcgcaactgc aggcccagat
     1741 cgatcacggt accgggcaca tcgccactgc cgcggcggcg cagcacgccc gggtgactgc
     1801 tgccggcgca gccgagaagg caaagctcgg cgcgacattc gccaaggcac acaatgacgt
     1861 cgctgcaagc gcagcccagg cgcacggcca gatcgctgcg cagagcaccg cacagggcgc
     1921 cgcactcacg aactggcaca ccggcgcggt tgcagccgcg aacgcctcgt tcgcccgggg
     1981 acagacacgc gcgcgcaagg cgggagacga tggcgcgcag aaggcgacga ccgcggcgaa
     2041 ccgggctgcc gcaacgatcg atagcaagct caaagaccac tcgcaacagg cgcatgcgat
     2101 cggtgcgcag aaggcgggcg cgggtggtgc acccgaggtc gccgacgcca aggccaaagc
     2161 ggccagcaag atctccgggg atgccgcaca gaagatcgga gatctcggcc acgtcaccgg
     2221 cgagctccga gccatggcgc cgtcgacggc cagcggcttg cggcaaaaag gcgaggaggc
     2281 cgcacgcgcg atcgctaccg gcggcccgca ggtgactgcg cagtacggcg cggtgcatgc
     2341 gagcaccgca caggcgatgc aggcgctcac cggcaagggc gcgcagagcc tcgatcagat
     2401 gaaggcacag accgcggcgg gtctgcatgc gcatcatcag cgcgcgatca caggcctcga
     2461 tcagcacacc gcgaagtcgg cggccgagat caaggccgcg ggcagccacg ccggcgcggc
     2521 ctatcgcaag cagggcgtcg agaccatgca cgccagcgag caggcgctcg gtgcgctcaa
     2581 gcagaaggtc gcggcgcagc cgatcgatcc gcagcgggca gccgccgcag ggacgcaggc
     2641 caaggctgag atccagcgcg ggttcgacgg cattgcgggc aaggcgcacg cgccggtcga
     2701 tcagatgacg tcgcatatca gcgcggcagg cgagcacgcc gttggggggc tcggcaaggt
     2761 gcacggccag atccaaccgc aggtcgccgg tgtcgtcgac catgcgcgcg gcagcggcag
     2821 caagctcgcc agcggtgtcg gtagcaagct cgcaggcgcg atgaccggcg cacgcggggc
     2881 aggctcgcag atgcaagcgc aggtcgctgc cggcatcgac aaccaggtcg gaaaggtcga
     2941 caaggcagtc gacaagggcg tgggcgatct gcagaccggg ctcgatgcca aggtctccga
     3001 ggccgaccag aaggcgcaac agccgctcaa ccaggtcggc ccgaagatcg atcaggcaca
     3061 agccaagatc gaggccgacg cacacaagag cacgctcgac aaggtgctga gctgggtcgg
     3121 cgaccagctc aagcagctcg gacgtatgct cctcgatcct gggttctggg tcgggctgct
     3181 ggtcgccgtc gtgctgatcg cgctgctccc tgaggagctc gctggtctcg cgctgatctg
     3241 cgcgatggcc gcgatcggtg cggtcgccgc cggcatcggc acgctcgtgt cgaacatcgc
     3301 ggctggacga cctccgctgc agaacgtcct gcaaaacatg ctcgtcggag cgttgttcgg
     3361 gggagcgctg acattcgttg gggctgcgct cggaaccgga cttgtcggga tcgcggcgat
     3421 gatgggcacc gccggcgtgc tcaccgtgat cgccaacctg gccaccggca agccgtggga
     3481 cgagggtcta ctcgcgaatg tcctcctggt cggcgtgttc gccggcatcg cgaagctgtt
     3541 cggacggccc ggagcgctga ggtccggcaa agaccccaag cccggtgatc cggtcacccc
     3601 ctccgacccg gtggagcctg gaaagggtgc tggaggcaat ggaacagcct cggagttcgg
     3661 cgatctaagc aacatgacgc gagccgaggc cgacacgttt cttcggtctc gtggtgcgac
     3721 ggtgaagcag actgcggggg ggtatacgga gtacaagttc cccgacaagt cagtggtctg
     3781 gatcaggcca gatggggaga tcgtccgcac accagctccg aaatacggtc cggatggacg
     3841 gaggatcaac aaaggcgcaa ggctcgatca gaatggcaac ccgactgata gccacaatac
     3901 cggagaacga gtcaagggtg atagcccatc tgaccaacct cccgaagaca agtgaatata
     3961 tggactacac ttctcttggc ctgggtccag ccccttcgct gatcaccgcg cttcacgtgt
     4021 cgcggtgggg cacagatgta cgcgtagtct gcagctacga tccgcagaac tcgcggagac
     4081 catacgagtt gcactttcaa aactgccgtc gcatccagtg ggatgtgcac gatgccgagg
     4141 gagcggagga tgagtcggcg gagctgatcg gtctgctcct cggcgaagga gggcacggcg
     4201 caccggccat tcttaccacc gatatcttcg agttgcagat cctctatggg agcttccact
     4261 tgcggaagga ctggtgaaga ggcccggcga gtgaaagcga cggcgcagcg ggggagagac
     4321 cgaacgcttg ttctcgcgcg tcggggggac ggtcacggcg atgcccggtc gccgtcgccg
     4381 ctcgagatca gcctggcgag catggacacc acgcatgtca cgatgcgttc gttctgatcg
     4441 acccgggcga gcatcatcgc gccctcgatc ccggcgagga gggtctgcgc cagcgcctgc
     4501 gcggacgccg tgtcccagcc gtcgttcacc aggcgccccg cgatgtgctc gcgccactcg
     4561 tcgaacgcct cccggatcgc ggcgtgtagc ttcggtagat cggggaccgc gccgacggcg
     4621 gtcgacgcca gcatggtgcc ctcgcgccac gccgagtcgc gcagccagcg cgccatgccg
     4681 gcggccatga cgcggagggt gtcctcgagc gagcgcccgt cggcctgcag tttgcgcagc
     4741 agcgccagca cgtcgccgcg aatccgctcg accgccgcga tcgcgatctg ctccttgccc
     4801 agcgggaagt ggtggtacat cgagcccttc ggcgctcgtg cctgctccag gatgtcggcg
     4861 gtgctcaccg cgtggtaacc gcgccgctgg aacagctgcg tcgcggcgaa cacgatgcgg
     4921 tcgcgggtcg gcacggtgcc cggcgctggc gtcagccgcg cggcgcgtgc tccggcggtc
     4981 atgatcggtt cgcgtcgacg atgcgaacgc tgaagctggc gttggcccgc gcgatcggcc
     5041 ggtcaccgct ggtcgccagc gcatcgcaga acccgaggct cgcgccggcg cggatcacgc
     5101 gcggcacgat gatcaccaga tcgccgagcc gcgcgctgtc gagatagtcg agcgccaggc
     5161 tgatggtcac gacgttcgct tcggcgcgca gcgccgtggc cagcgacagc cccatcacgt
     5221 tgtcgcacag cgcggccagc acgccgccgt gcagcagccc gcgcgagttg cagtgcgcgg
     5281 gctggacctc gaacgccagg tctacgccgt ccgcccggcg gcgcgagtac agcggctccc
     5341 agggcgccgt gaccggactc ttgcggaagt ggggctcgaa ctcggcagga atggtcacca
     5401 tggcccgaga ttaccttaca tagaccgatc ggtctaggta atatggaagg tgctacgcgg
     5461 cgcgcgtgta gtgcaggccg acgacgccgg tcgggtaggg cgtcgccttc gcgagcgcga
     5521 accggcggtc gatgccggtc gccggaaaca cgcgcttgcc gcggccgagc gcgagcggat
     5581 agacgtccag gtagagctcg tcgacgaggt cgtgctcgaa cagcgtgtga agcagctggc
     5641 tgctcccgtc gagcaggatc gccttgccgg cggccgcctt gagcctgcgc actgcgccga
     5701 cggcgtcatc gcggatgatc gtcgtgttcc tccagatcgg cttgtcgagc gtcctcgaca
     5761 cgacgtactt cgccggcgcg ttcatcatgt cgccgaacgg atcgccggcc ggcatcggct
     5821 cgaacgcctg cgcgtgggtg acgtacgtgc gacgcccgag caggaacgcg tcggcgtcct
     5881 gcatgatggc gccgaacttc gtgccgatgt catcgtgcca gtacggccgg ctccagccgc
     5941 cgtgggtgaa gccgccgtcg cggtcctcgt cgggaccgcc gggcgcctgg aggatgccat
     6001 cgagggtgac gaacccgtga acgatcagct tgcgcatcgg taactccttt cggtttgatc
     6061 gggcgacgaa cgagcgcggc ccggatcgac acccggcgat gcgctttccg gatctcgacc
     6121 ggacgaccgg gccaggtatg gaaattcagg tagatgcgcg caggtcgacc gggcgcgccc
     6181 gcgcggtcca tgccttatgc tgcgcgcgat gcggtacacc gcgctgctgc tcgacctcga
     6241 tcacacgctg ctcgactccg acgcctcgga gcggctggcg ttcgagcacg cgctggccgg
     6301 cgccggggtg gccgacccgg cgggctactt tccggcgtac gacgcgatca accgtgcgct
     6361 gtgggcacgc gtcgagcgcg gcgagatcca cgcgacgcag gtccgggtcc agcggttcgc
     6421 gcagctcatc gcggccaccg gcatcgccgc ctcgccggac gcgctcgccg acgcgtatgc
     6481 cagcggcctg tgcgcccacg gcgagctcta tcccggcgcg cgcgacgtgc tcgacgcgct
     6541 cgacgccgag cccgcggtgt cgctcgcgct ggtcaccaac ggcctgtccg aggtccagcg
     6601 cgcgcggatc gatcggctcg gcctcggccg ctacttcgac gtgatcgcga tctccgccga
     6661 gctcgggacg gcgaagccgg cctccgcgat cttcgagttc gcgctcgccg ggctgggcgc
     6721 cccggccagg gccgcggcgc tgatggtcgg cgatagcctg acctcggaca tccagggcgg
     6781 ccgcaacgcc ggcatcgcga cctgctggta caacccgcat cgccggccgc gcgaccagga
     6841 cgggctcgtc gatcacgaga tcgccacgct cgatgcgcta ctggcactcg tacgctcgta
     6901 gcggatgcgg agcgccgcag atcatgtact cgctacggga tcgcgacgcc gcacgagtag
     6961 gacgtcgtcg tgagatccgc ccagtactgc gagatcactc gctcgaacgg ctcaccgaac
     7021 ctcgcgcgcg catacaccgg cacaccgatg gggtcgacga gctggccgag cgcgcagtcg
     7081 gtgcagccac cgctgcggat gtcgtcctcg tcggggatgg tgaacgccgg gtcgacgagc
     7141 tcgcagtggt cctggccata gccggcgtag tccgcgatct cgaggaatgg ctcgtcgcgc
     7201 gtcgcgctca tgtacagctt gtagctcttc gacgagccgc acaggatggc accgacccag
     7261 acaccgtagt gatcatcatg gcggacgaat cggtcgccgg tgcaccccgg cgtggacagc
     7321 gtggagaaat cgagcctctc ccagccgagg cacgtgccga atcgatcata ctgctggtag
     7381 ccggccgacg tgttgcagtt cgaggtcgcc gtgccgtcgg cgtggagcgt ccagctcgag
     7441 gcctggtagc agccgtggaa cgtcgtgagc cccacgccgg cctgagcctc cgcgagcgcg
     7501 aggagctggt tcgtatcgca cccgccgccg ttgtaggggt acgaataacc acacgcagtg
     7561 ccgcgcgtga ccgggacgga tcccaggtcc acggtttgcg ggtcgtagcc gaggagctcg
     7621 acggcgcgaa cggtcgcgcc cgtcgcgagg cggacgtgcc agttcgtcgc gacgtatgcc
     7681 gagaggacga ggatatgctc gcccgggcgt tcgatcacga cgctggcgtc tcctgtcaca
     7741 ctgtgattgc cgctggtctc gtacatggcg atcacgtgga gctccgggac gccgcagctt
     7801 cccggcggcg agccgccggc atccggcgga accggagaac cgccgtcgca cgcaccgccg
     7861 ccgggacggc cgccgtcggg aaggccgccg gggcgaccgc cgtcgggtgg gctgccgccg
     7921 ggacgaccac cgtcgggtgg gccaccgtgc tcgggagcat cggaaccgaa gtagagcgag
     7981 caacctgcga gtgacagggc gaccagcgct acgagcctca tctgcctcca gcagagccaa
     8041 tccgcgcgcc gctgtctacc aagaaccgct cgggcatcga ttcgcaccgt cagcgtcgtc
     8101 tgaccggccg acggaacacg gggcctggcg gtcggcccgt cgtgtcgtgt gcagatggca
     8161 cccgacctcg agtactgccg cgttttactg cgggtgatct tgtgccggcg gaccggcgat
     8221 gttatcgagg gtgacggggg catgccgcgt ttcgagtcca ggtccgggcg atttgtcgag
     8281 ctggagatcg aggacacggt gctgacgcag gtcgagggaa cggtcgacgg ctcgtatgtg
     8341 gggaagccgt cgacgcgcac attctcgacc gcggatgagg cgcgctatgc ggcggcacgg
     8401 tggatcgccc gacgccgcaa gcgcgacggc tatcagctgg tcggtcaagc gcgccacctc
     8461 ggcggcggtc cgggcacgat cccacgtccg ccggggcccg tgtcggtgat cgcggcccgt
     8521 gcctgctgga ctgccgcgga tccgcaattc ctgtccgagc tgttgcgccc ggcggccgcg
     8581 gcgcatgccg ccgggctggc cgggctcgcc gagcgctggt tccgggatga ccggccgtgg
     8641 gcgcgcgaca tgctgcgggc gtacatcgac gatggctgcg atcggccgtg gcacaaggcg
     8701 ctggtcaagc ggctgttcaa gctggccgag gcggccggcg acgacgagct gatgacgcac
     8761 ttcctggtgg cgttcgatcg gctgtctcgc cggtacctga tcaaggagtc gaagtacgcg
     8821 ggctgggatc gcgcggcgcg gcgcgcgatc gtgctcgagt cgatgcgcct ggtgcaggat
     8881 ccgtcgatcc cggcgcggct ccccgagcgc gaggccacgg ccggaggcaa gggcaaggcc
     8941 ggtgcgcgga accaacccgg gcgcaagcgc cccgcagagg agccgcagtt cacgcgggtg
     9001 acacggcgct acctggcgcg gcgcgcgtac cgctacttcc ggatgctcgc gcaccgcgac
     9061 ctcgcgcgct acggccgcgc gatgcggctc gcattgccgc gctacaccga cgagtcgctc
     9121 tcgaccgcga cccgcctgct cgacgcgtgg ggcctgatgc acgcgctgta cgcgcgctcg
     9181 ccggcgatcg tgcgctcgcc cgccgggatc cggctcgccg atggcaagac cctcgcggac
     9241 ctggcgccgg cgccgcactt cgatgcggcg tgggacggcg tgttctccga gctgctcgcg
     9301 ctcgcgagcg atgccggcag ccgcaccgtc cgggggtggg cggtggcgct gctccgcgcg
     9361 cgctacgccg gcgacctggc ggcgctggcg ttcccggcga tcaagcggct ggtgatgagc
     9421 ccgcacgacg aggtcgtggc gctggggatc gagcggttga cccggatgtc cgggctggag
     9481 acggtcgcgc tgcacgactg gctcgatctg ctcgcggtgc agaacctcga cgtgttgccc
     9541 gcgatcgccg agctcgccga gcggtcgctg tcccccgcgc ggcttccgct cgcgcagtgc
     9601 atcgacctcg cgtgcggcaa gaccgcgccg gtcgcgcgcc tcggcctggt gtgggcgaag
     9661 accagggcga tccagaccgc cgacgatctg cgcgcgatcg cgcggctcgc ccgcgccggc
     9721 gtggcgacgg tgcgcgacga aggcacggcc tgggcgctgg aggtgatcgc ggcccacccg
     9781 gcgacgcgac ccgacgagct gcgcg
//
LOCUS       DASVOR010000174         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_147769,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000174 DASVOR010000000
VERSION     DASVOR010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147769"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VF516_03935"
     CDS             <1..465
                     /locus_tag="VF516_03935"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8106850.1"
                     /translation="VPEARQLFPFMTVEENLELGAYNRRSRPRARDTRDEMLELFPRL
                     KERRRQLAGTLSGGEQQMVAIARGLMAAPRLLLLDEPSLGLSPKVTEEVFGRVQDIRK
                     RGVTVLIVEQNVVDALSIADRGYVVENGRIVMQGDAANLLRDEKVRAAYLGL"
     gene            520..837
                     /locus_tag="VF516_03940"
     CDS             520..837
                     /locus_tag="VF516_03940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106851.1"
                     /translation="MTFDFHTALVVCALIASVLLVLHGGQRLCALIALVACALEALSA
                     FGVLHLSSPKVRIDIILSAVLVVTGAICWMRSAGKTAITAATVVALVGLIQLLQSHVL
                     HGF"
     gene            863..1774
                     /locus_tag="VF516_03945"
     CDS             863..1774
                     /locus_tag="VF516_03945"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8106852.1"
                     /translation="MSLEILAEGLGFPEGPIAMADGSVILVEIKRQTLSRCWNGRTEV
                     IATLGGGPNGAAIGPDGAVYVCNNGGSVWIERHGITFPIGIAPDYAGGRIERVDLATG
                     KVDRLYDRCGDHPLRAPNDLVFDRAGGLWFTDFGHDLGRARDKSGIFYCAPDGSRIVE
                     VYFGSLGYNGIGLSPDDATLYAAETLTGRLVAFDVTGPGQIARRGNRFPGRVVATMSG
                     NPPEYFDSLAVTAAGNVCVATLLAPGITTIAPDGNYSKLETPDVFTTNLCFGGADLRD
                     AWITLSGTGKLARTRWPEPGLRLAFNA"
     gene            1786..3039
                     /locus_tag="VF516_03950"
     CDS             1786..3039
                     /locus_tag="VF516_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8106853.1"
                     /translation="MDFTIPPEIALYLAELDAFIEREIVPLEREHPQYFDHRREHART
                     DWDDDGKPRRAWEELLAEMRRRADRAGHLRFALPRELGGQDGSNLAMAIIREHLAHKG
                     LGLHNDLQNESSIVGNFPQVHLMHRFGTAAQKARFLEAMITGTEHIAFGLTEPNHGSD
                     ATWLETTAVKSGTDWVINGQKRFNTGLHLATTDLIFARTSGKPGEARGITAFLVPTRA
                     PGFEVPFMWWTFNMPTDHAEVRLTNVRVPDDAILGGEGEGLRVAQAFVHENRIRQAAS
                     SLGVAQYCIDQSVAYARQRITFGKPLAANQAIQFPLAELHTECAMVRSLVHRTAWHLD
                     RQDHMEVSDLVSMANYRANRLACDAADRAIQVHGGVGYSRHYPFEHIYRHHRRYRITE
                     GSDEIQLRKIAGHLFGFMTRGRSAE"
     gene            3036..>3878
                     /locus_tag="VF516_03955"
     CDS             3036..>3878
                     /locus_tag="VF516_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX8106854.1"
                     /translation="MTEHEVFQHTLEAAIRRRLGAPGEIRDLVRLTGGATKLTWSFDA
                     VVGADTLPLILQQTPPDRLAAASELGGSRVHGAQDAALLDAAASAGVPAPRVRLALEP
                     DDHLGEGVVTDRVAGETLCRKINTDPRLAAVRPRLAAQCGAILAAIHRIDPTDLPFLA
                     TQTAADQLAIYRGIADACDHPLPAVELGLRWVAEHLPAAPRTSVVHGDFRNGNLIVGD
                     DGVRCVLDWELAHLGDPMLDLGWICVKTWRFGGGPPVGGFGHRDDLFRAYEQAGGGPV
                     DPTHV"
BASE COUNT          558 a         1524 c         1263 g          533 t
ORIGIN      
        1 gtccccgagg cccgccagct gttcccgttc atgaccgtcg aggagaacct cgagctcggc
       61 gcctacaacc ggcgcagccg cccgcgggcg cgcgacaccc gcgacgagat gctcgagctg
      121 ttcccaaggc taaaggagcg ccgccgccag ctcgccggga cgctgtcggg cggcgagcag
      181 cagatggtcg cgatcgcccg cggcctgatg gccgccccgc ggctgctcct gctcgacgag
      241 ccgtcgctcg gcctgtcgcc caaggtcacc gaggaggtgt tcggccgggt ccaggacatc
      301 cgcaagcgcg gcgtcaccgt gctgatcgtc gagcagaacg tggtcgacgc gctcagcatc
      361 gccgaccgcg gctacgtggt cgagaacggc cggatcgtca tgcagggcga cgccgcgaac
      421 ctcttgcgcg acgagaaggt ccgggcggcg tacctcggcc tgtaacctac attggaatcg
      481 cggcccggtt cgcgcgatgc tcggcgccag gaggacctga tgaccttcga tttccacacc
      541 gccctcgtcg tgtgcgcact gatcgcctcg gtcttgctcg tcctgcacgg gggccagcgg
      601 ttgtgcgcgc tgatcgccct ggtcgcctgc gcgctggagg cgctgagcgc gttcggcgtg
      661 ctccatctgt cgtcgcccaa ggtccggatc gacatcatcc tgtcggccgt gctcgtggtg
      721 accggcgcca tctgctggat gcggtcggcg ggcaagacgg cgatcaccgc ggcgacggtg
      781 gtcgcgctgg tcggcctgat ccagctgctc cagtcgcacg tgctgcacgg gttctagcga
      841 cgccatgccg gaggcacctg ccatgagcct ggagatcctc gccgaggggc tcggcttccc
      901 cgagggtccg atcgccatgg ccgacggctc ggtgatcctg gtcgagatca agcgccagac
      961 cctgtcgcgg tgctggaacg gccggaccga ggtcatcgcg acgctgggcg gcggccccaa
     1021 cggcgcggcg atcggccccg acggcgcggt ctacgtgtgc aacaacggcg gctcggtgtg
     1081 gatcgagcgc cacggcatca cgttcccgat cggcatcgcg ccggactacg cgggcggccg
     1141 gatcgagcgc gtcgacctcg cgaccggcaa ggtcgaccgg ctgtacgacc gctgcggcga
     1201 tcacccgctg cgcgccccca acgacctcgt gttcgaccgc gccggcggcc tgtggttcac
     1261 cgacttcggc cacgacctcg gccgggcgcg cgacaagagc ggcatcttct actgcgcccc
     1321 cgacggctcg cgcatcgtcg aggtctactt cggctcgctc ggctacaacg gcatcgggct
     1381 gtcgcccgac gacgccacgc tgtacgccgc cgagaccctg accggccggc tggtggcgtt
     1441 cgacgtcacc ggcccgggcc agatcgcgcg ccgcggcaac cggttccccg gccgcgtggt
     1501 cgcgaccatg agcggcaacc cgccggagta cttcgacagc ctcgcggtca ccgccgccgg
     1561 caacgtgtgc gtcgcgaccc tgctcgcgcc cggcatcacc acgatcgcgc ccgacggcaa
     1621 ctacagcaag ctcgagactc ccgacgtgtt caccaccaac ctgtgcttcg gcggcgccga
     1681 cctgcgcgac gcatggatca cgctgtcggg caccggcaag ctcgcccgta cccggtggcc
     1741 cgagcccggg ctgcggctcg cgttcaacgc ataggacctc gcgccatgga cttcaccatc
     1801 cctcccgaga tcgccctgta cctcgccgag ctcgacgcgt tcatcgagcg cgagatcgtg
     1861 ccgctggagc gcgagcaccc gcagtacttc gatcaccgcc gcgagcacgc gcgcaccgac
     1921 tgggacgacg acggcaagcc gcgccgcgcg tgggaagagc tgctcgccga gatgcgccgc
     1981 cgcgccgacc gcgccggcca cctccggttc gcgctgcccc gcgagctcgg cggccaggac
     2041 ggctcgaacc tcgcgatggc gatcatccgc gagcacctcg cgcacaaggg cctcggcctg
     2101 cacaacgatc tgcagaacga gtcgtcgatc gtcggcaact tcccgcaggt ccacctgatg
     2161 catcggttcg ggaccgcggc ccagaaggcg cggttcctcg aggcgatgat caccggcacc
     2221 gagcacatcg cgttcggcct gaccgagccc aaccacggca gcgacgcgac ctggctcgag
     2281 accacggcgg tcaagagcgg caccgactgg gtgatcaacg gccagaagcg gttcaacacc
     2341 gggctccacc tcgccaccac cgacctgatc ttcgcgcgga cctccggcaa gcccggcgag
     2401 gcgcgcggca tcaccgcgtt cctcgtcccg acccgcgcgc ccggcttcga ggtcccgttc
     2461 atgtggtgga cgttcaacat gccgaccgac cacgccgagg tccggctgac caacgtccgc
     2521 gttccggacg acgcgatcct cggcggcgag ggcgagggcc tgcgcgtcgc ccaggcgttc
     2581 gtccacgaga accggatccg ccaggcggcg tccagcctcg gcgtcgccca gtactgcatc
     2641 gatcagagcg tcgcctacgc gcgccagcgc atcacgttcg gcaagccgct cgccgccaac
     2701 caggcgatcc agttcccgct cgccgagctc cacaccgagt gcgcgatggt ccgcagcctg
     2761 gtccaccgca ccgcctggca cctcgaccgc caggaccaca tggaggtcag cgacctggtg
     2821 tcgatggcca actaccgcgc caaccgcctc gcgtgcgacg ccgccgaccg ggcgatccag
     2881 gtccacggcg gcgtcggcta ctcgcggcac tacccgttcg agcacatcta tcgccaccac
     2941 cgccgctacc ggatcaccga gggctccgac gagatccagc tccgcaagat cgccggtcac
     3001 ctgttcggct tcatgacccg ggggcgcagc gcggaatgac cgagcacgag gtgttccagc
     3061 acacgctgga ggccgcgatc cgccgccgcc tcggcgcgcc cggcgagatc cgcgatctgg
     3121 tgcgcctcac cggcggcgcg accaagctca cctggtcgtt cgacgccgtg gtcggcgccg
     3181 acaccctgcc gctgatcctg cagcagaccc cgcccgaccg cctcgccgcc gcgtccgagc
     3241 tcggcggctc gcgcgtccac ggcgcccagg acgccgcgct gctcgacgcc gccgcctcgg
     3301 ccggcgtccc cgcgccccgg gtccggctcg cgctcgagcc cgacgatcac ctcggcgagg
     3361 gcgtcgtcac cgaccgcgtc gccggcgaga ccctctgccg caagatcaac accgacccgc
     3421 gactcgccgc cgtgcgcccc cggctcgccg cccagtgcgg cgcgatcctc gccgccatcc
     3481 accgcatcga ccccaccgac ctgcccttcc tcgccaccca gaccgccgcc gatcagctcg
     3541 cgatctaccg cggcatcgcc gacgcctgcg atcacccgct ccccgccgtc gagctcggcc
     3601 tgcgctgggt cgccgagcac ctgccggccg ccccgcggac ctcggtcgtc cacggcgact
     3661 tccgcaacgg caacctgatc gtcggcgacg acggggtgcg ctgcgtcctc gactgggagc
     3721 tcgcccacct cggcgacccg atgctcgacc tcggctggat ctgcgtcaag acctggcggt
     3781 tcggcggcgg cccgccggtc ggcggcttcg gccaccgcga cgacctgttc cgcgcctacg
     3841 agcaggccgg cggcggcccg gtcgatccca cccacgtc
//
LOCUS       DASVOR010000175         5918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_498563,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000175 DASVOR010000000
VERSION     DASVOR010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498563"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VF516_03960"
     CDS             <1..191
                     /locus_tag="VF516_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinyl-CoA--3-ketoacid-CoA transferase"
                     /protein_id="HEX8106855.1"
                     /translation="TKKNEHKLVRACDYPLTAARVVSLVITDLGVFQPTGDGFRVVDL
                     APGVTRDYAQARTGAPLR"
     gene            197..451
                     /locus_tag="VF516_03965"
     CDS             197..451
                     /locus_tag="VF516_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX8106856.1"
                     /translation="MKTKVSVSLPRDLLEETDKEAAQTSRSRNQILEIWLRLAGRRRA
                     EQKLEQDTLAHYASRTVGQEAEDEALSQASLRAATKRKID"
     gene            complement(544..1569)
                     /locus_tag="VF516_03970"
     CDS             complement(544..1569)
                     /locus_tag="VF516_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106857.1"
                     /translation="MCAEPSRSAEMGVVGHRAPRPPALRGTPRSVCRKLARRLRIGAD
                     GAVGAKRVIWHVGFERNLQRRGPRSFEIRSEVPLSDEPPRLDYLLLRKLAAEGAPDES
                     AETLRRLWALLPRVTVMEYKSPGRPYRPGNLDRLWGYVHTYFANERSLPRKRADGTTL
                     AAGMDGAPEVWTREDLCAVLVVPTRTPALTADAEAMGLAWNGLGSGYWRVRGGLFVLY
                     VAEIDVIGRAEGDDLLCSLGHGQLVTPETRRFWMELVGTQQAGISMQDMEGYDELMQK
                     LLATLPPEQRLAGLAPEQRLAGLDRDHQALALPIEVLRLLPNEYLRSLSPEVQAEIRR
                     RLTQNGH"
     gene            complement(2430..3935)
                     /locus_tag="VF516_03975"
     CDS             complement(2430..3935)
                     /locus_tag="VF516_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106858.1"
                     /translation="MNLPIEVDLLSRISVPLFVHEAHRETWMTACLIGPDVALTSASL
                     GAYGNQPFEIQVEGKSHTGRLEIFDDDVGIALVRFDPAVQSDLPQIFSTREQTVLPGA
                     GSLTLANLGHDRSISLSGTVLRSTTRNGKPFYVVGQSQTINDVGGISGAPVIIEGRVL
                     GLISGVVLPGRSGGLSTFLVAPFSALRGTPLAERLAALGLADHAADPESPPSPVLTPA
                     DPEDARLFAMLSPRARLALSHADAMRRAMQLESVHIEQLVAALQQQSSNLAADALAAA
                     GLDEQGLRDLVAQITGAALPEAVQSATLSTLPPLSVHARDAVVAASRLSSDGIIHLRD
                     LLAGAMSVTGSSFIRALIELGVRVPPVRAPTAADANAAPRAAPDPAGTTAPAPAATIS
                     TLATALAGFRSDGVSDQDDLLDIERDVAALCSVIAARDVEPPRSARRCAGERAATSPR
                     DRGVGAGVHEAVPRADRDAARGQAVREHLPAAQGTRPRRTGRRVPRGRVRR"
     gene            4108..5703
                     /locus_tag="VF516_03980"
     CDS             4108..5703
                     /locus_tag="VF516_03980"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8106859.1"
                     /translation="MRDFEQRSKGDSASREAGSGSPAAPGKSTLTEQIFRQASGAAVD
                     AEAASTPGALGGLGGSTGSALPAGPRARLETSLGADLSGVRVHTGAASQAAADQLGAR
                     AFTTGRDIHFAAGQYRVDDLFGMHLLAHEVAHTQQQGGQAGLQTKLRISEPGDAAEQA
                     ADAFADHIVHGGPAPSVGSAPAGVIHRVPAAPSYGGVTAVSTRGAITIDAVADFVAST
                     LTAARPVHAHTTDARVTHLSWELYNPSDTMIGGFCTLPGNASSLTEPFNLEPRYFSGT
                     GFAPGRYILRVSGLNAQHQPVIYTDRNVNVMAADQTTGTGTSTGHGTITFTRYDKTDG
                     THTNPAYNVSAEIRFTPDATAGLQDVVFMQATQTQDAQGNSQHNTTSVDQDARQTPLG
                     WSIDRIAGAPGPFYIEGYVVRRRGGRNQRVVEDIPGWGQAGRSNGAGHASTPATLIDT
                     PNWNHENFFKAEAVAMCRSGPNRGQIYGACTWGYTATSSGQVTLMPRSVHPLPSDQFT
                     EAKNAWNDWQSRQPAATAPARAP"
     gene            5711..>5918
                     /locus_tag="VF516_03985"
     CDS             5711..>5918
                     /locus_tag="VF516_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106860.1"
                     /translation="MSAAALVLLLVVAVAGCDHRATPAPSGGSQATMTQPSSTPMPPD
                     LAALERALADDTARDVPPFGADSVR"
BASE COUNT          916 a         2101 c         2075 g          826 t
ORIGIN      
        1 tgaccaagaa gaacgagcac aagctggtcc gcgcctgcga ctacccgctc accgcggcgc
       61 gcgtggtctc gctcgtgatc accgatctcg gcgtgttcca gccgaccggc gacggcttcc
      121 gcgtcgtcga tctcgccccc ggcgtgaccc gcgattacgc ccaggcccgg accggcgcgc
      181 cgctgcgctg atgggtatga aaacgaaggt cagcgtctcg ctccctcgtg acctcctgga
      241 ggagacagac aaggaagccg cccagacgtc gagatcacgt aatcagattc tcgagatctg
      301 gttgcggctg gccggccgcc gccgagctga acagaagctc gagcaagata cgcttgccca
      361 ttacgcgagc cgcacggtcg ggcaggaagc cgaggacgag gcgctgtcgc aggcgtcgct
      421 ccgcgcggcc acgaagcgga agatcgacta gcccatgctt caaccgctgc cgtggtggcg
      481 accgataaat gcaagcctgg tggacgggga gttcggccgt ctgactgatc gccggctcgt
      541 tcgtcagtga ccgttctgcg tgagccggcg gcgaatctcg gcctggacct ccggtgagag
      601 cgaacggagg tactcgttgg gcaagaggcg cagcacctcg atgggcagcg ccagcgcctg
      661 gtgatcgcgg tcgagcccgg cgaggcgctg ctcgggtgcg agcccggcga ggcgctgctc
      721 gggcggcagc gtggcgagca gcttctgcat cagctcgtcg tagccttcca tgtcttgcat
      781 gctgatcccc gcttgctgcg tgccaacgag ctccatccag aaccttcgcg tctcgggcgt
      841 gacgagttgc ccgtgcccca gagagcatag cagatcgtcg ccttccgcac ggccgatcac
      901 gtcgatctcg gccacgtaca gcacgaacag cccgccccgg accctccagt agccgctgcc
      961 caggccattc cacgccagcc ccattgcctc cgcatccgcg gtcagggccg gcgtgcgcgt
     1021 aggcaccacc agcaccgcgc agagatcctc gcgcgtccag acctccgggg ccccgtccat
     1081 gcccgccgcg agcgtcgtgc cgtcggcccg cttgcgaggc aggctgcgct cgttggcgaa
     1141 gtaggtatga acgtagcccc agagtcgatc gagattgccc ggacgatagg gacgaccagg
     1201 gctcttgtac tccatcaccg tcacgcgggg caacagcgcc cacagccggc gcagggtctc
     1261 ggcgctttcg tccggcgcgc cctcggcggc gagcttgcgc agcagcaggt aatcgaggcg
     1321 gggcggctcg tccgagagcg ggacctccga gcgaatctcg aatgaacgtg ggccgcgtcg
     1381 ctggaggttt ctctcgaagc cgacgtgcca gatgactcgc ttggcgccca cggcgccatc
     1441 tgcgccgatc ctgagtcgcc gggcaagctt tctacagacc gagcgaggcg ttcctcggag
     1501 cgcgggtggc ctcggcgcgc gatggccgac cactcccatc tcggcggaac gcgacggctc
     1561 ggcgcacacg gcgagtgccg ccgcgtgatc ggaggcagtg tcagctggtt agcgagaagc
     1621 cagcagcgtc agcgtcggct tggtcaccgg tgctgcagtg ccctcgccaa ctgggcggtc
     1681 cccggcggcg aggtcgtggg catcgggcag cgcgatggac ctcgcgtgcg gctgccgcca
     1741 cgtggtcgcg atgatgcagc acgtgaccaa gaagaacgag cacaagctgg tccgcgcctg
     1801 cgactacccg ctcaccgcgg cgcgcgtggt ctcgctcgtg atcaccgatc tcggcgtgtt
     1861 ccagccgacc ggcgacggct tccgcgtcgt cgatctcgcc ccccggcgtg acccgcgatt
     1921 acgcccaggc ccgcaccggc gcgccgctgg gctgatcggc ggctcagctg gcttccgggc
     1981 ggtcgcctgg ccgagcggcc ggccggggtg ccggaggctc cggcgggagg accgtggcgc
     2041 tgagccggcc ggcctggaac gagaatcgcg cgacgtacgg ggtccagaac cggaacacct
     2101 cggtcgacgg cagggcgaga tcctggtcgg cgaagcgccg gcgaagcagc gcgagcttgg
     2161 cggcgagctc gtccagccgc tcctcgtgcg ccgaccgcgc cgcggcttcg tccggccgtc
     2221 ccggtggcgc ggacctcgcg tccggctcgg gcggttgctc gtgctgcgcg gacctcgccg
     2281 ccgccttctg cttttcgctg gcgaggtacg tcaacagcca cggccaccag ccctcggccg
     2341 cgggctcctc ggtgaggtcg cgcatcatgt cggtggtctc cgacggatag ccgatcagca
     2401 cggcgagcag gaagatcgcc gcgcggtgct caccgccgga cgcgtccccg cggaacgcgg
     2461 cggcccgttc gtcggggacg cgtgccttga gcagccggta gatgttcacg aaccgcttgg
     2521 ccgcgcgcgg cgtcgcgatc agctcgtgga actgcttcat gaacgcctgc tcccacgcct
     2581 cgatcacgag gtgaggtggc agcacgttca ccggcgcacc ggcgggccga gcgcggaggt
     2641 tcgacgtcgc gcgccgcgat caccgagcac agcgcggcga cgtcgcgctc gatgtccagc
     2701 aggtcgtcct ggtcggacac gccgtcggag cgaaacccgg cgagcgcggt ggcgagcgtg
     2761 gagatcgtgg cggcgggcgc gggcgccgtg gtgccggcgg ggtccggcgc cgccctgggt
     2821 gcggcgttgg catcggcggc ggtcggcgcc cgtactggcg gaacccgcac tccgagctcg
     2881 atgagcgcgc ggatgaagct cgacccggtg accgacatcg cgccggccag cagatcgcgc
     2941 aggtggatga tgccatcgct gctcagcctg gatgctgcga ccaccgcgtc gcgcgcgtgc
     3001 accgatagcg gtggcagtgt ggagagcgtg gctgactgta cggcctcggg aagcgcagcc
     3061 ccggtgatct gcgcgaccag atcgcgcagc ccctgctcgt cgagccccgc agcggccagc
     3121 gcgtcggccg cgaggttgct cgactgctgc tgcagcgctg cgaccagctg ctcgatgtgc
     3181 acgctctcga gctgcatcgc gcggcgcatc gcgtcggcgt gtgatagcgc gagccgcgct
     3241 cgcggcgaca gcatcgcgaa cagccgggcg tcctcggggt ccgccggcgt gaggacaggg
     3301 ctggggggcg actccggatc ggcggcatga tcggcgagcc ccagcgcggc caagcgctcc
     3361 gcgaggggcg tgcctcgcag cgccgagaac ggggcgacga ggaacgtcga caggcccccg
     3421 ctgcggcccg ggagcacaac tccgctgatc agcccgagga cacgaccctc gatgatgacc
     3481 ggagcaccag agattcctcc tacatcgttg atcgtctggg actgtcccac gacgtagaac
     3541 ggcttgccgt tccgggtggt cgatctaagc accgtgccgg atagcgatat cgaccggtcg
     3601 tggccgaggt tcgcgagcgt gagggacccc gcaccaggta ggacggtctg ctcgcgcgtg
     3661 gagaatatct gaggcaggtc cgactggacc gccggatcaa atcgcaccag cgcgatgccg
     3721 acgtcgtcgt cgaagatctc gagccgaccc gtgtgactct tgccttcgac ctggatttcg
     3781 aacggctggt tgccgtacgc gccgagcgac gccgaggtca gtgcgacatc ggggccgatg
     3841 aggcacgccg tcatccaggt ctcgcgatgc gcttcgtgca cgaacagcgg caccgagatc
     3901 ctgctcagca gatcgacttc gataggcaga ttcatgtttc ccgaccacgc tcgcccgtgg
     3961 cgcacgcgcc aggctaccga agtcccggac cgcgattcaa gctgcaccgg cggtgcgccg
     4021 ccgaatggag cggccgcccg ctgcgcgatc agcaggtaga tccagctgcc tttcgtcgta
     4081 caatggtacg cagcccaagg cggtctcatg cgggatttcg agcaacggag caagggggac
     4141 tcggcgagcc gcgaggcggg ctcggggagc ccggctgccc ccggcaagtc gacgctgacc
     4201 gagcagatct tccggcaggc gagcggcgcg gccgtcgatg ccgaggccgc atcgacgccg
     4261 ggcgcgctgg gcgggctggg cggctcgacc ggcagcgcgc tgcccgcggg gcccagggcg
     4321 cggctcgaga ccagcctcgg cgccgacctg tccggggttc gcgtccacac cggcgcggcg
     4381 tcgcaggctg cggccgacca gctcggcgcg cgcgcgttca ccaccggccg cgacattcac
     4441 ttcgccgcgg ggcagtaccg ggtcgacgat ctgttcggca tgcacctgct cgcccacgag
     4501 gtcgcgcaca cccagcagca aggcgggcaa gccggattgc agaccaagct ccgcatcagc
     4561 gagcccggcg atgccgccga gcaggcggcg gatgcgttcg ccgatcacat cgtgcacggc
     4621 ggcccggcgc cgagcgttgg cagcgcgccc gccggcgtga tccaccgcgt gccggcagcc
     4681 ccgagctacg gcggcgtcac cgcggtctcg acccgcggcg cgatcacgat cgacgcggtc
     4741 gccgacttcg tcgccagcac gctgacggcg gcgcgcccgg tgcacgcgca cacgaccgac
     4801 gcgcgggtca cccacctgtc ctgggagctc tacaacccgt ccgacaccat gatcggcggg
     4861 ttctgcacgc tgccgggcaa cgccagctcg ctcaccgagc cgttcaacct cgagccgcgc
     4921 tacttcagcg gcaccggctt cgcgcccggc cgctacatcc tgcgggtctc ggggctcaac
     4981 gcccagcacc agccggtgat ctataccgac cgcaacgtca acgtgatggc ggcggatcag
     5041 acgaccggca ccgggacgtc gaccggtcac ggcacgatca cgttcacgcg ctacgacaag
     5101 accgacggca cgcataccaa cccggcgtac aacgtgagcg ccgagatccg gttcacgccg
     5161 gacgcgacgg ccggcctgca ggacgtcgtg ttcatgcagg cgacgcagac ccaggacgcg
     5221 cagggcaaca gccagcacaa caccaccagc gtcgatcagg acgcgcggca gacgccgctc
     5281 ggctggtcga tcgatcggat cgccggtgcg ccggggccgt tctacatcga gggctacgtg
     5341 gtgcggcggc gcggcggccg caaccagcgc gtcgtcgagg acatcccggg ctggggccag
     5401 gccgggcggt ccaacggggc gggccacgcg tcgacccccg cgacgctgat cgacacaccg
     5461 aactggaacc acgagaactt cttcaaggcc gaggcggtcg cgatgtgccg cagcggtccc
     5521 aaccgcggcc agatctacgg cgcctgcacg tggggttaca ccgcgacctc gagcggtcag
     5581 gtgaccctga tgccgcgctc ggtccatccg ttgccctcgg accagttcac cgaggccaag
     5641 aacgcgtgga acgactggca atccaggcag ccggcggcga ccgcaccggc ccgggcgccg
     5701 tgacgcgatc atgtccgccg cggccctggt cctgctcctg gtcgtcgccg tcgccggctg
     5761 cgatcaccgg gcgacgcccg caccatcggg cgggagtcaa gcaaccatga cccagccgtc
     5821 ctcgacaccc atgccccccg atctcgcagc gctggagcgc gcgctggccg acgataccgc
     5881 gcgcgacgtg ccgccgttcg gcgccgacag cgtgcggc
//
LOCUS       DASVOR010000176         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_439988,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000176 DASVOR010000000
VERSION     DASVOR010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439988"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="VF516_03990"
     CDS             complement(<1..287)
                     /locus_tag="VF516_03990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8106861.1"
                     /translation="MYNEEQVLPLLRQQLTQLINELLCRVEVILVDDGSSDRTYALMH
                     EWALVEPAIKLIALSRNFGHQIAITAGMDASSGDAVVIMDADLQDPPSLIP"
     gene            complement(546..1520)
                     /locus_tag="VF516_03995"
     CDS             complement(546..1520)
                     /locus_tag="VF516_03995"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8106862.1"
                     /translation="MSLRIGILGAAKIAGSFMVGAKASSRVAVVAIASRDRARAEAFA
                     RHHDIPRAYGYAELLADREVDAIYNPLPNALHAEWSIAAARAGKHVLCEKPLAGSEAE
                     AEAMFAAADAHRVVLVEAFPYMFQPQTLEIERLIASGAIGEVRTMFAAFGFTVAEPGN
                     IRLDARLGGGALMDAGCYPVSFARQVFRARPIRVTATARWLGSVDQTLAATLEYPGGG
                     IAQISCSFATAVHRRAIIAGTTGVIDTDYHNHTDRVAAPSYRVKRSADWQAEFETVPV
                     ASENGFRAELDAFAEIIERNDRAAVVARRIASLDNAWTLAAILTAARR"
     gene            1716..>1921
                     /locus_tag="VF516_04000"
     CDS             1716..>1921
                     /locus_tag="VF516_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106863.1"
                     /translation="MTHSLTLAQARALWWHKQALAGTGKQPLAALIGDSGWLRTLGGA
                     DAYLAARARRPGMTRAELDAAIAAG"
BASE COUNT          286 a          703 c          663 g          269 t
ORIGIN      
        1 tccgggatca gcgacggcgg gtcctgcagg tcggcgtcca tgatcaccac ggcatcgccc
       61 gacgacgcat ccatccccgc cgtgatcgcg atctggtgcc cgaagttgcg cgacagcgcg
      121 atcagcttga tcgccggctc cacgagcgcc cactcgtgca tcagcgcgta cgtccgatcg
      181 gagctgccat catcgaccag gatcacctcg acccggcaca gcagctcgtt gatcagctgg
      241 gtcagctgct gccgcagcag cggcaacacc tgctcctcgt tgtacatggg gataacgatc
      301 gacagcagcg ccggcgattc gcgtggcagc aacatctccc gggcggggag tatgcacggt
      361 tatcgccgcg gtgggagcgt cgagacgacg actatttgtc gccggtggac cgccgggccc
      421 tcgccaccct ggcaggaatc caccacccgg gtgttggcag ttccacagcg tgaaccccag
      481 atcgtgtcga tccagtcaca cgccccggca cggtcacatc cccccggcaa ccgccgccga
      541 acgcgtcacc ggcgcgcggc ggtgaggatc gcggcgagcg tccacgcatt gtcgaggctg
      601 gcgatgcggc gggccacgac cgcggcgcgg tcgttgcgct cgatgatctc ggcgaacgca
      661 tcgagctcgg cgcggaagcc gttctcgctc gccaccggca cggtctcgaa ctcggcctgc
      721 cagtcggcgc tgcgcttgac ccggtagctc ggggcggcga cccggtcggt gtggttgtgg
      781 taatcggtgt cgatcacgcc ggtcgtgccg gcgatgatcg cgcggcggtg gaccgcggtc
      841 gcgaagctgc agctgatctg cgcgatgccg ccccccgggt actccagcgt ggcagccagg
      901 gtctggtcga cgctgcccag ccagcgcgcg gtggcggtga cccggatcgg ccgggcgcgg
      961 aacacctggc gcgcgaagct caccgggtag cagccggcgt ccatcagcgc gccgccgccg
     1021 agccgggcgt cgagccggat gttgcccggt tcggcgacgg tgaagccgaa cgcggcgaac
     1081 atcgtgcgga cctcgccgat cgcgcccgac gcgatcagcc gctcgatctc gagggtctgg
     1141 ggctggaaca tgtacgggaa cgcctcgacc agcacgacgc ggtgggcgtc ggccgcggcg
     1201 aacatcgcct cggcctcggc ctcgctgccg gcgagcggct tctcgcacag cacgtgcttg
     1261 ccggcgcgcg ccgcggcgat cgaccactcg gcgtgcagcg cgttgggcag cgggttgtag
     1321 atcgcgtcga cctcgcggtc ggcgagcagc tcggcgtagc cgtacgcgcg cgggatgtcg
     1381 tggtggcgcg cgaacgcctc ggcccgcgcg cggtcgcggc tcgcgatcgc caccaccgcg
     1441 acgcgcgacg acgccttggc gccgaccatg aacgacccgg cgatcttcgc cgcaccgaga
     1501 ataccgatcc ggaggctcat cgcgcgcgag gctatcaggc cggcacgccg agcacgaatg
     1561 aagaagtcga ttcgcgcagg ccgtgcatcg atcggcctgg atcgctgctt cgtccccacc
     1621 cagcatcaga tcgacctgac gcgcccgagc tactccgggc gcacaccgcc gcttgacgat
     1681 tcgcgccgat cgcccgatca acaggagacc cgcccatgac ccattcgctc acgctcgcgc
     1741 aggcgcgcgc cctgtggtgg cacaagcaag ccctcgcggg caccggcaag cagccgctcg
     1801 ccgcgctgat cggcgactcc gggtggctgc gcacgctcgg cggcgccgac gcctacctcg
     1861 ctgcacgcgc ccgccggccc ggcatgacgc gcgccgagct cgacgccgcg atcgccgccg
     1921 g
//
LOCUS       DASVOR010000177         2010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_527726,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000177 DASVOR010000000
VERSION     DASVOR010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527726"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2010
                     /locus_tag="VF516_04005"
     CDS             <1..>2010
                     /locus_tag="VF516_04005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX8106864.1"
                     /translation="QIKDADYAPAFEAGMAEQRKEIDAIAHNPEPPTFDNTIVALERS
                     GQTLTRVENTFGNLNASNTDEAMEKVESEMAPRLAAHRDAILLDAALFARVDAVYQHR
                     GELGLDPESAQLVERYEDMFVRAGARLSDADKATLKKLNEELSSLSTRFRQDVLAATK
                     DGAVVVDDVQQLDGLSQEQIGAAAEAAKARGLDGKWVITLQNTTIQPPLAQMKNRALR
                     ERVFRASVMRGRGGATDNTGVVSRIVELRAKKAALLGYPTFAAYALADETAGTPAAVN
                     RILDQLAPAALAKAREEAAGIQAQIRADARAAHTQPFTLAPWDWAFYAQQVRKARFSF
                     DDAQVKPYFEMNRVLQDGVFFAAHELYGLTFTERHDLPVYQPDVRVFEVRDADGSTIG
                     LLLLDYFKRDNKQGGAWMDTFVDQSTLLGDKPVVINNLNVPKPAPGAPALLTFDDVTG
                     MFHEFGHALHGLLSAVKYPLLSGASVPPDFVEFPSQFNEMWSREPAVLAHFAKHYQTG
                     EPMPRALLDKILAAKDYGQGYATLEYLAASLLDQSWHQIPLASVPPAARVMAFEDAAL
                     ARHRVAYPPVPPRYHTTYFSHVFAGGYEAGYYAYIWSEVLARDAGAWFHAHGGLSRAA
                     GDTFRAKILSRGRTKEPSVLFEEFYGHPPDIKPLLEYRGLTLPGQP"
BASE COUNT          316 a          697 c          721 g          276 t
ORIGIN      
        1 ccagatcaag gacgccgact acgcgccggc gttcgaggcg gggatggcgg agcagcgcaa
       61 ggagatcgac gcgatcgcgc acaaccccga gccgccgacg ttcgacaaca cgatcgtcgc
      121 gctcgagcgc agcgggcaga cgctgacgcg ggtcgagaat acgttcggga acctcaacgc
      181 gtcgaacacc gacgaggcga tggagaaggt cgagtccgag atggcgccca ggctggcggc
      241 gcaccgcgac gcgatcctgc tcgacgcggc gctgttcgcg cgggtcgacg cggtgtatca
      301 gcaccgcggc gagctcgggc tcgatcccga gtcggcgcag ctggtcgagc gctacgagga
      361 catgttcgtg cgcgccggtg cgcggctgtc cgacgccgac aaggcgacgc tgaagaagct
      421 caacgaggag ctgtcgtcgc tgtcgacccg gttccgccag gacgtgctcg cggcgaccaa
      481 ggacggcgcg gtcgtggtcg acgatgtcca gcagctcgac gggctgtcgc aggagcagat
      541 cggggcggcg gccgaggcgg cgaaggcgcg cgggctcgat ggcaagtggg tgatcacgct
      601 gcagaacacg acgatccagc cgccgctcgc ccagatgaag aaccgcgcgc tccgcgagcg
      661 cgtgttccgc gcgtcggtca tgcgcggccg cggcggtgcc accgacaaca ccggcgtggt
      721 gtcgcggatc gtcgagctgc gggcgaagaa ggcggcgctg ctgggctacc cgaccttcgc
      781 ggcctacgcg ctggcggacg agaccgcggg gacgccggcc gcggtcaacc ggatcctcga
      841 ccagctggcg ccggccgcgc tggccaaggc gcgcgaggag gcggccggca tccaggccca
      901 gatccgcgcg gacgccaggg cggcgcatac ccagccgttc acgctcgcgc cgtgggactg
      961 ggcgttctac gcccagcagg tccgcaaggc gcggttcagc ttcgacgacg cgcaggtcaa
     1021 gccgtacttc gagatgaacc gcgtgctcca ggacggcgtg ttcttcgcgg cgcacgagct
     1081 ctacgggctc acgttcaccg agcgccacga tctgccggtc tatcagcccg acgtgcgggt
     1141 gttcgaggtc cgcgacgcgg acggctcgac gatcggcctg ctcctgctcg actacttcaa
     1201 gcgcgacaac aagcagggcg gcgcgtggat ggacacgttc gtcgatcagt cgacgctgct
     1261 cggcgacaag ccggtggtga tcaacaacct gaacgtgccc aagccggcgc ccggcgcgcc
     1321 cgcgctgctg acgttcgacg acgtgaccgg gatgttccac gagttcgggc acgcgctgca
     1381 cggccttctg tcggcggtca agtacccgct tttgtccggc gccagcgtcc cgcccgactt
     1441 cgtcgagttc ccgtcgcagt tcaacgagat gtggtcgcgc gagccggcgg tgctcgcgca
     1501 cttcgcgaag cactaccaga ccggcgagcc gatgcccagg gcgctgctcg acaagatcct
     1561 cgcggccaag gactacggcc agggctacgc cacgctggag tacctcgcgg cgtcgctgct
     1621 cgatcagtcg tggcaccaga tcccgctcgc gagcgtgccg cccgccgcgc gggtgatggc
     1681 gttcgaggac gccgcgctcg cccggcaccg cgtcgcgtac ccgccggtgc cgccgcgcta
     1741 tcacacgacg tacttctcgc acgtgttcgc cggcggctac gaggccggct actacgcgta
     1801 catctggagc gaggtgctcg cgcgcgacgc cggcgcgtgg ttccacgccc acggcgggct
     1861 gtcgcgggcc gcgggcgata cgttccgcgc caagatcctg tcgcgcgggc ggaccaagga
     1921 gccgagcgtg ctgttcgagg agttctacgg ccaccccccg gacatcaagc cgctgctgga
     1981 gtaccgcggg ctgacgctgc ccggccagcc
//
LOCUS       DASVOR010000178         4925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_498587,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000178 DASVOR010000000
VERSION     DASVOR010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4925
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498587"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VF516_04010"
     CDS             complement(<1..1008)
                     /locus_tag="VF516_04010"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106865.1"
                     /translation="MIEPHDPLIGTVLDQRFRIEHQLAAGGFGAIYRATDVLSDAQVA
                     LKVLLPRFTRDPMVVARFRREGQTLASLRDPHTITAYELGEAPDGTLYIVMELLLGES
                     LYTRFRNAGPMPWRRVLHIAAGVCSSLAEAHALGVVHRDLKPANIHLEFHDGDPDFVK
                     VLDFGIAKIVQGTDLEHTQLTQAGQMIGTVDYMSPEQMVGGELTPASDIYTLGVVIYE
                     MIAGRTPFADCQTATAILAAVLTRAPEPLSTYARVPVLLDRIVARCLERDPQSRFTDV
                     SDLVEALAEVTAGGAARQTPAAPVNEFAQPVLTTEATRIDVRFNESSQPVLDARGARR
                     RL"
     gene            1225..2385
                     /locus_tag="VF516_04015"
     CDS             1225..2385
                     /locus_tag="VF516_04015"
                     /inference="COORDINATES: protein motif:HMM:NF005071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8106866.1"
                     /translation="MKRLVVLALLAACRNTHDEPRPSPPPPAAPTPAPAGAGTTAAPA
                     APKPIGGVTPIAGDFQIKDFAFTSGEKLPELRIHYRTLGTPKRDAAGHVTNAVIVLHG
                     TTGSGQQFARKQFADELFGPGQPLDLTRTYVILPDDIGHGDSSKPSDGMHARFPHYGY
                     HDMVEAEHALVVDGLHVDHLRLVMGTSMGCMHAWMWAEKWPDAMDAVMPLACLPTQIA
                     GRNREWRKMVTDAIKQDPAWNGGEYTQQPRGALLDASHLLTLAGSSPIADQKRLATRD
                     AADQAVEKAEAEFVATHDANDVLYAVDSSRDYDPSADLEKITAQLLFVNSADDFINPP
                     ELGVAEQMIKRVKHGRFVLIPASEQTHGHGTHTWATFWKKDLQQLLDATAKK"
     gene            2477..3076
                     /locus_tag="VF516_04020"
     CDS             2477..3076
                     /locus_tag="VF516_04020"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HEX8106867.1"
                     /translation="MGEIDEKISDALDKARESRLNTVIAALVAVTATLMALNNVKDGN
                     IVQNMQQAQASSVDEWAYYQAKGTKQNFAVAAREQLEIMRDATANPTPELRAMFEKRI
                     AEYVAQEKKYEAEKEEIRRKAEGFQKQYDELNRHDDQFDAAEALMSVAIALFGVTALT
                     RKRWLLVVGILFAGTGEILGLAGFLGWSLHSDWLASVLS"
     gene            complement(3086..>4925)
                     /locus_tag="VF516_04025"
     CDS             complement(3086..>4925)
                     /locus_tag="VF516_04025"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8106868.1"
                     /translation="SRRWKAGEDAKDRLKDILEEVSAKRDWPKASVEQLIGDFYGGCM
                     DQARIDARGAEPIRPLLAEIDQLREPADVGRMIARLHKIGIFAPFGLFGNSDLHTPSN
                     VVAWATAGGLGLPDRDYYVKTETLFVERREKYLAHVAAMFKLAGRDDAAAHRAADTVM
                     AIELGLARASLDNVARRDPTARDHTMSAAELQALTPSFDWKAYFASAGIHPASLNVTE
                     PAFFTEVERQLGATPVADWKTYLAWHVINAAAPALSQAFVDEDYAFNSKYLAGTEEIK
                     PRWKRCVELTDPLLGEALGKKYTDKYFPPAAKARMQQLVGNLLAAMHDTIDQLDWMSA
                     ATRQRALDKLATFNAKIGYPDKWKDYSSIPVRRDQFWETVLAAQAWNVADNRATIDKP
                     VDRGRWLLTPQTSDAYYSASLNEIVFPAGILQPPAFSLDAVDAVNYGAIGVVIGHEIS
                     HGFDDQGAQFDAQGRLDNWWTPGDLDQFHARGQCVADQFEHYFIEPGIHHTGKLVLGE
                     SIGDLGGANIAYRAFQKAKQQTPAPVLDGFTPDQQFFIAWGQFRSDAIRPETQRLMVQ
                     GDPHPIAQYRVIGPLSNMTEFAAAFSCKAGAAMVRPAAERCVVWGK"
BASE COUNT          775 a         1742 c         1661 g          747 t
ORIGIN      
        1 cagccggcgg cgcgcgccgc gcgcgtcgag caccggctgc gacgactcgt tgaaccgcac
       61 gtcgatccgg gtcgcctcgg tcgtgagcac cggctgcgcg aactcgttca ccggcgcggc
      121 gggcgtctgc cgcgcggcgc cgccggccgt gacctcggcg agcgcctcga ccagatcgct
      181 gacgtcggtg aaccggctct gcggatcgcg ctccaggcag cgcgcgacga tccggtcgag
      241 cagcaccggg acccgggcgt acgtcgacag cggctcgggc gcccgggtca gcaccgccgc
      301 gaggatcgcc gtcgcggtct ggcaatcggc gaacggcgtc cgcccggcga tcatctcgta
      361 gatcacgacg ccgagcgtgt agatgtcgct cgccggcgtc agctcgccgc cgaccatctg
      421 ctcgggcgac atgtaatcga ccgtgccgat catctgtccg gcctgggtca gctgggtgtg
      481 ctcgaggtcg gtgccctgga cgatcttggc gatcccgaaa tcgagcacct tgacgaagtc
      541 cggatcgccg tcgtgaaact ccaggtggat gttggccggc ttgagatcgc ggtgcacgac
      601 gccgagcgcg tgggcctcgg cgagcgagct gcacaccccg gccgcgatgt ggagcacgcg
      661 gcgccacggc atcgggcccg cgttgcgaaa ccgcgtgtac aggctctcgc cgagcagcag
      721 ctccatcacg atgtacagcg tgccgtcggg cgcctcgccg agctcgtacg ccgtgatcgt
      781 gtgtggatcg cgcaggctcg ccagcgtctg gccctcgcgc cggaaccgcg ccacgaccat
      841 cggatcgcgc gtgaacctgg ggagcagcac cttgagcgcg acctgggcgt cggacagcac
      901 atccgtcgcg cggtagatcg cgccgaaccc acccgctgcc agctggtgct cgatccggaa
      961 ccgctgatcg aggaccgtcc cgatgagcgg atcgtgcggt tcgatcaccg tgcgatcgta
     1021 cgcttccccg tcttcggcgg tgcgaatcat acgtcccttt cggtatacgc aacggccgac
     1081 aacgtagctg tattcgtgac agacttttga acacgcagat gtaacattca tacgttcgac
     1141 gcagggaagt atgacggact gcgtcacgta gcccggagat ctggcatagc cagcagcgcg
     1201 gccgttcggc tagcttccgg ggccatgaag cgcctggtcg tcctcgcctt gctcgccgca
     1261 tgtcgaaaca cccacgatga accgcggccg tccccgccgc cgcccgctgc cccgacccct
     1321 gccccggcgg gcgcaggaac caccgccgct cccgcggccc ccaagccgat cggcggcgtg
     1381 acgccgatcg caggcgactt ccagatcaag gacttcgcgt tcaccagcgg cgagaagctg
     1441 cccgagctgc gcatccacta caggacgctg ggcaccccca agcgggacgc cgccggccac
     1501 gtcaccaacg ccgtgatcgt cctgcacggc acgaccggct cgggccagca gttcgcgcgc
     1561 aagcagttcg ccgacgagct gttcggcccc ggccagccgc tcgacctgac ccgcacctac
     1621 gtgatcctgc ccgacgacat cggccacggc gattcgtcca agccgtccga cggcatgcac
     1681 gcccggttcc cgcactacgg ctaccacgac atggtcgagg ccgagcacgc gctggtcgtc
     1741 gacggcctcc acgtcgatca cctgcggctg gtgatgggca cctcgatggg ctgcatgcac
     1801 gcctggatgt gggcggagaa gtggcccgac gcgatggatg cggtcatgcc gctcgcctgc
     1861 ctgccgaccc agatcgccgg ccgcaaccgc gagtggcgca agatggtgac cgacgcgatc
     1921 aagcaggacc cggcgtggaa cggcggcgag tacacccagc aaccgcgcgg cgcgctgctc
     1981 gacgccagcc acctcctgac gctcgccggc tcgtcgccga tcgccgacca gaagcggctg
     2041 gcgacccgcg acgccgcgga ccaggcggtc gagaaggccg aggccgagtt cgtcgcgacc
     2101 cacgacgcca acgacgtgct ctacgcggtc gactcgtcgc gcgactacga cccctcggcg
     2161 gacctcgaga agatcaccgc ccagctgctg ttcgtgaaca gcgccgacga cttcatcaat
     2221 ccgcccgagc tcggcgtcgc cgagcagatg atcaagcgtg tcaagcacgg ccggttcgtg
     2281 ctgatcccgg cgtcggagca gacccacggc cacggcacgc acacctgggc gacgttctgg
     2341 aagaaggacc tccagcagct gctcgacgcc accgccaaga aatgacattc ggggcaacct
     2401 gccccgacta ccgatctcgc agccgccgcc gcgtcgatcg gcttgggcgc aggcgcgagc
     2461 gcgctagcat gcggccatgg gtgaaatcga cgagaagatc agcgatgcgc tcgacaaggc
     2521 gcgcgagtcg cgactcaaca ccgtgatcgc cgcgctggtc gcggtcaccg ccacgctgat
     2581 ggcgctcaac aacgtcaagg acggcaacat cgtccagaac atgcagcagg cccaggccag
     2641 cagcgtcgac gagtgggcgt attaccaggc caagggcacc aagcagaact tcgcggtcgc
     2701 cgcgcgcgag cagctcgaga tcatgcgcga cgccaccgcc aacccgacgc ccgagctgcg
     2761 cgcgatgttc gaaaagcgca tcgccgagta cgtcgcccag gaaaagaagt acgaggccga
     2821 gaaggaagag atcaggcgca aggccgaggg cttccagaag cagtacgacg agctcaaccg
     2881 ccacgacgac cagttcgacg ccgccgaggc cctgatgtcg gtcgcgatcg ccctgttcgg
     2941 cgtcaccgcg ctgacccgca agcgctggct gctggtcgtc ggcatcctgt tcgcgggcac
     3001 cggcgagatc ctcggcctcg ccggcttcct cggctggagc ctgcactccg actggctggc
     3061 cagcgtgctc agctgaccgc ggcggtcact tgccccacac cacgcagcgc tctgcggctg
     3121 gccgcaccat cgccgcgccc gccttgcacg agaacgcggc ggcgaactcg gtcatgttgg
     3181 acagcgggcc gatcacccgg tactgcgcga tcgggtgcgg atcgccctgc accatcagcc
     3241 gctgggtctc gggccggatc gcgtcgctgc ggaactggcc ccacgcgatg aagaactgct
     3301 gatccggcgt gaagccgtcg agcaccggcg ccggggtctg ctgcttggcc ttctggaacg
     3361 cgcggtaggc gatgttggcg ccgccgagat cgccgatgct ctcgccgagc acgagcttgc
     3421 cggtgtggtg gatgccgggc tcgatgaagt agtgctcgaa ctgatcggcc acgcactggc
     3481 cgcgggcgtg gaactggtcg aggtcgcccg gggtccacca gttgtcgagc cggccctggg
     3541 cgtcgaactg cgcgccctga tcgtcgaacc cgtggctgat ctcgtggccg atcaccacgc
     3601 cgatcgcgcc gtagttcacc gcgtcgaccg cgtcgagcga gaacgccggc ggctgcagga
     3661 tgccggccgg gaacacgatc tcgttgagcg atgcgctgta ataggcgtcc gaggtctgcg
     3721 gcgtcagcag ccagcggccg cggtcgaccg gcttgtcgat cgtcgcgcgg ttgtcggcga
     3781 cgttccacgc ctgcgcggcc agcacggtct cccagaactg gtcgcggcgg accgggatgc
     3841 tggagtagtc cttccacttg tcggggtagc cgatcttggc gttgaacgtc gcgagcttgt
     3901 cgagcgcgcg ctgcctggtc gcggcgctca tccagtcgag ctggtcgatc gtgtcgtgca
     3961 tcgccgcgag caggtttccg accagctgct gcatccgcgc cttggcggcc ggcgggaagt
     4021 acttgtcggt gtacttcttg ccgagcgcct cgccgagcag cgggtcggtg agctcgacgc
     4081 agcgcttcca gcgcggcttg atctcctcgg tgcccgcgag gtacttgctg ttgaacgcgt
     4141 aatcctcgtc gacgaacgcc tgcgacagcg ccggcgccgc cgcgttgatc acgtgccacg
     4201 ccaggtaggt cttccagtcg gcgaccggcg tcgccccgag ctggcgctcg acctcggtga
     4261 agaacgccgg ctcggtgacg ttgaggctcg ccgggtggat cccggccgac gcgaagtacg
     4321 ccttccagtc gaagctcggg gtcagcgcct ggagctcggc cgccgacatc gtgtgatcgc
     4381 gcgcggtcgg atcgcgccgc gcgacgttgt cgagcgaggc cctggcgagc ccgagctcga
     4441 tcgccatcac ggtgtccgcc gcgcggtgcg ccgcggcgtc atcgcgcccg gcgagcttga
     4501 acatcgcggc gacgtgcgcg aggtacttct cgcgccgctc gacgaacagg gtctcggtct
     4561 tgacgtagta gtcgcggtcg ggcagcccga ggccgccggc ggtcgcccag gcgaccacgt
     4621 tgctcggcgt gtggaggtcc gagttcccga acaggccgaa cggcgcgaag atcccgatct
     4681 tgtgcagcct ggcgatcatc cggccgacgt cggcgggctc gcgcagctgg tcgatctcgg
     4741 cgagcagcgg ccggatcggc tcggcgccgc gcgcatcgat ccgtgcctgg tccatgcagc
     4801 cgccgtagaa gtcgccgatc agctgctcga cgctggcctt cggccagtcg cgcttcgccg
     4861 acacctcctc gaggatgtcc ttgagccggt ccttggcgtc ctcgcccgcc ttccagcgcc
     4921 ggctc
//
LOCUS       DASVOR010000179         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_30876,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000179 DASVOR010000000
VERSION     DASVOR010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30876"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VF516_04030"
     CDS             <1..982
                     /locus_tag="VF516_04030"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106869.1"
                     /translation="GGRRLDRAYFRELKKRLIERQAQGMLEFVEPRWDLATVVGHDAA
                     KRRLLDDAELIKRGELDTVPMGYLFCGPVGTGKSFLATCVAGSIGIPAVVLKNFRSKY
                     VGETEGNLERVLGVLRSMGPVVVIVDEADAMLGDRDQGGDSGVGARTFGMIAAQMGDT
                     AYRGRILWMLLTARPDLLPIDLKRQGRAEVHIPLFYPTDDAELRTMLMVLARKAGTEL
                     AESDAPAEIPHKGNLSGADIEGIVGRAYRASLLAGSRRITPGALAAALAGFMPSTQTL
                     EREAQELAAIIECTDVEFLPPAKRDKLDKLGGREKLQERLTAIKQILDGQ"
     gene            999..1289
                     /locus_tag="VF516_04035"
     CDS             999..1289
                     /locus_tag="VF516_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106870.1"
                     /translation="MARASWLNDDSHPDLDAHLASLDHFAASLADGVIDDHELATQET
                     NLVAAMKAVEGSLSDDQHAKVTKLLAELTAYSVMRTLHDMAQARVQQVVAPK"
     gene            1354..1869
                     /locus_tag="VF516_04040"
     CDS             1354..1869
                     /locus_tag="VF516_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106871.1"
                     /translation="MRAVFNQLERRIEDRWSVPVHIQDVPNPFTGDLDGEQIMVDYDL
                     DIEDAVFILIHLFGHTVQWNVSPGARAVGFLQPTTWTDDQLRAARDYEHEAARYSLQL
                     LHEAGIRDLDQWISDFAACDCAYLMHFYTTGEKRPFRSFWRDGTPLVAPLAIPEFQPT
                     QWLSRYQGTVV"
     gene            1898..>2327
                     /locus_tag="VF516_04045"
     CDS             1898..>2327
                     /locus_tag="VF516_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106872.1"
                     /translation="MLARRIIAVTPDKAFGKQLTVALKAAGGAVDTHLTLDELGHGEL
                     HAALLVVHLDGAMTGAAQVLLPRLTGETRVIAILPRSNLSAVVDIMQSSDRVAGMMVA
                     EDFDMKLLSAMATRVLAGDIFGLEKHVPWGAQIHSYLVGDY"
BASE COUNT          378 a          824 c          796 g          329 t
ORIGIN      
        1 gggcggccgc cggctcgacc gcgcctactt ccgcgagctc aagaagcggc tgatcgagcg
       61 ccaggcccag ggcatgctgg agttcgtcga gccgcgctgg gacctggcga ccgtggtcgg
      121 ccacgacgcc gccaagcgcc gcctgctcga cgacgccgag ctgatcaagc gcggcgagct
      181 cgacaccgtg ccgatgggct atctgttctg cgggccggtc ggcaccggca agagcttcct
      241 ggcgacctgc gtcgccggct cgatcggcat cccggcggtc gtgctcaaga acttccgcag
      301 caagtacgtc ggcgagaccg agggcaacct cgagcgggtg ctcggcgtgc tgcgctcgat
      361 gggcccggtc gtggtgatcg tcgacgaggc cgacgcgatg ctcggcgacc gcgatcaggg
      421 cggcgactcc ggggtcggcg cccgcacgtt cggcatgatc gccgcccaga tgggcgacac
      481 cgcgtaccgc ggccggatcc tgtggatgct gctgaccgcg cggcccgacc tgctaccgat
      541 cgacctcaag cgccagggcc gcgccgaggt ccacatcccg ctgttctacc cgaccgacga
      601 cgccgagctg cgcaccatgc tgatggtgct cgcgcgcaag gccggcaccg agctcgccga
      661 gtccgacgcg ccggccgaga tcccgcacaa gggcaacctg tcgggcgccg acatcgaggg
      721 catcgtcggc cgcgcctacc gcgcgtcgct gctcgccggc tcgcgccgga tcaccccggg
      781 cgcgctcgcc gcggcgctcg ccgggttcat gccctcgacc cagaccctgg agcgcgaggc
      841 ccaggagctc gcggcgatca tcgaatgcac cgacgtcgag ttcctgccgc cggccaagcg
      901 cgacaagctc gacaagctcg gcgggcgcga gaagctgcag gagcggctga ccgcgatcaa
      961 gcagatcctc gacggacagt agcaacaagg agcgaaccat ggcacgcgca agctggctca
     1021 acgacgacag ccaccccgac ctcgacgccc acctggcgag cctcgatcac ttcgcggcgt
     1081 cgctcgccga cggcgtgatc gacgaccacg agctcgcgac ccaggagacc aacctggtcg
     1141 cggcgatgaa ggcggtcgag ggctcgctga gcgacgacca gcacgccaag gtcaccaagc
     1201 tgctcgccga gctgaccgcg tacagcgtga tgcgcacgct gcacgacatg gcgcaggccc
     1261 gggtccagca ggtcgtcgcc ccgaagtgac cgcggccggg tcagggacca tgacgctccg
     1321 agggagccgg cggtgaagcc cgatcaggat cggttgcgag ccgtattcaa ccagctggag
     1381 cgccggatcg aggaccgctg gagcgtcccg gtgcacatcc aggacgtccc caacccgttc
     1441 accggcgacc tcgacggcga gcagatcatg gtcgactacg acctcgacat cgaggacgcg
     1501 gtgttcatcc tgatccacct gttcggtcac acggtgcagt ggaatgtctc gcccggggcg
     1561 cgggccgtcg ggttcctgca gccgaccacg tggacggatg atcagctccg cgccgcgcgg
     1621 gactacgagc acgaggccgc ccgctacagc ctgcagctcc tgcacgaggc cggcatccgc
     1681 gatctcgacc agtggatctc ggactttgcg gcctgcgact gcgcgtactt gatgcacttc
     1741 tatacgaccg gcgagaagcg gccgttccgg tcgttctggc gcgacggtac ccccctggtg
     1801 gcgccgctgg cgatcccgga gttccagccg acccagtggc tgagtcgtta ccaaggaact
     1861 gtcgtctgat cggcatttcg ggctacgatt tcccagggtg ctcgctcggc ggatcatcgc
     1921 tgtcacgcca gacaaggcgt tcggaaagca actgacggtc gcgctcaagg cggccggggg
     1981 cgcggtcgat acccacctca cgctcgacga gctcggccat ggcgagctgc acgcggcgct
     2041 gctggtggtc cacctcgacg gcgcgatgac gggcgccgcg caggtcctgc tcccccggct
     2101 gaccggcgag acccgcgtga tcgcgatcct gccgcgctcc aacctgtcgg cggtggtcga
     2161 catcatgcag agctcggacc gggtcgccgg gatgatggtc gccgaggact tcgacatgaa
     2221 gctgctctcg gcgatggcga cccgggtgct cgccggcgac atcttcgggc tggagaagca
     2281 cgtgccgtgg ggcgcgcaga tccacagcta cctggtcggc gactacc
//
LOCUS       DASVOR010000180         3923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_235471,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000180 DASVOR010000000
VERSION     DASVOR010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235471"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1334)
                     /locus_tag="VF516_04050"
     CDS             complement(<1..1334)
                     /locus_tag="VF516_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106873.1"
                     /translation="MTRPNRSTALFDVIHGLWETVAASVAEPQTVRSDDSVPSRSFRT
                     GRGEPVIGHSINEIFRIDGPPAFTFVEPPQFAEIRLALRTMGTGLIVEGPSKAGKSTA
                     IRKVMDDVAVPLGDQIWWHGHRMPPLAEFGRMLDRLLSATRDTWLFIEDVHRIANARY
                     RRELGSAMKVLADQSVRHAKITLIGNNPGGHSLLQWMPDLVGRLRVIRVDVAHDRGSN
                     IRIAELIGAGEAAANVRFRRRDEFVEAAAGSFFLAQYLCNVAALQSRVFEAQASVVKI
                     GIGPDDVIAALHDELAVHFRAPILAFARFDAALATPGAGLSLLWMLAHSSDGFAALRD
                     ARMRWPALGAAFDDLPANLGRCFQDHPELDGLLHYEGVTERLVVEDLALRLYLRGLRW
                     DDLVKATGHGRVRFHPVDGPEWPVSPAGAALPAGPASPAGQVAPAGQIAAAGR"
     gene            complement(2038..2916)
                     /locus_tag="VF516_04055"
     CDS             complement(2038..2916)
                     /locus_tag="VF516_04055"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEX8106874.1"
                     /translation="MALGNLAQRFLVAVVAVPILLALVYYHRPEPTWALIFGASLIAM
                     HEAFAMLLPAEDRRAALVLGGIATAAFYWLDPMTLAGYGVPPAVLVGAEGTGQAVPLA
                     LAVVAPGLYFLFRFRDIPSVAGRYAATVTGIVYAGYLLTYLAKLKLIERFQPGEGGDA
                     VLIVLIVAWVADTGAYFAGRFLGKARLYEAVSPKKTWAGAWGGLAGSLAGVVALKLIS
                     AHWLSWLDVVLLAIPGGVLGQLGDLTESLIKRSVGVKDSGALLPGHGGLLDRIDAVLF
                     IAPYAYAYLVIRHTIG"
     gene            complement(2999..3745)
                     /gene="uppS"
                     /locus_tag="VF516_04060"
     CDS             complement(2999..3745)
                     /gene="uppS"
                     /locus_tag="VF516_04060"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEX8106875.1"
                     /translation="MSTEPRKLPRHVGIIMDGNGRWAERRGLPRLDGHRVGADSVRDV
                     TRAARELGIEALTLYAFSSQNWQRPVDEVHGLMQLLRDYLLGERAEILDNAIRLEAIG
                     DVERLPPMVREPLDELRAASAANHGMTLTLALSYGGRESIARAVRAAVRDAVLGRLSP
                     DQVDVERFSGYLPTAELPPLDLLIRSSGEQRISNFMLWEIAYAELVFVDVLWPEFRRE
                     HLYACLDQYAARERRFGLTTGQLGSDVESS"
BASE COUNT          627 a         1378 c         1392 g          526 t
ORIGIN      
        1 cgaccggccg cggcgatctg gccggccggg gcgacctggc cggcggggct cgcagggccg
       61 gccggaagcg ctgcgccggc ggggctcacc ggccactcgg gcccatcgac gggatggaac
      121 cgcacccggc cgtggccggt cgccttgacc aggtcgtccc agcgcagccc gcgcagatag
      181 agccggagcg cgagatcctc cacgaccagc cgctcggtca cgccctcgta gtggagcagc
      241 ccgtcgagct ccgggtgatc ctggaagcac cggccgaggt tcgccggcag gtcgtcgaac
      301 gcggcgccga gcgcgggcca ccgcatgcgc gcgtcgcgga gcgcggcgaa gccatcgctg
      361 ctgtgggcga gcatccacaa caggctcagc ccggcgcccg gcgtcgcgag cgcggcatcg
      421 aaccgcgcga acgcgaggat cggcgcgcgg aagtgcaccg cgagctcgtc gtggagcgcc
      481 gcgatcacgt cgtccggtcc gatcccgatc ttgaccaccg acgcctgggc ctcgaacacc
      541 cgggactgca gcgccgcgac gttgcacagg tactgggcga ggaagaagct gccggccgcg
      601 gcctcgacga actcgtcgcg gcgccggaac cggacgttgg ccgccgcctc gccggccccg
      661 atcagctctg cgatccggat gttcgatccg cgatcgtgcg cgacgtcgac ccggatcacg
      721 cggagccggc cgaccaggtc cggcatccac tgcagcagcg agtggccgcc cgggttgttg
      781 ccgatcagcg tgatcttggc gtggcggacc gactgatcgg cgagcacctt catcgccgag
      841 ccgagctcgc gccggtagcg cgcgttcgcg atccggtgga cgtcctcgat gaacagccag
      901 gtgtcgcggg tcgcggacag caggcgatcg agcatgcggc cgaactcggc gagcggcggc
      961 atccggtgcc cgtgccacca gatctgatcg ccgagcggca ccgcgacgtc gtccatgacc
     1021 ttgcggatcg ccgtcgactt gcccgccttc gacggtccct cgacgatcag cccggtgccc
     1081 atcgtgcgca gcgcgagccg gatctcggcg aactgcggcg gctcgacgaa cgtgaacgcg
     1141 gggggtccgt cgatgcgaaa gatctcgttg atgctgtgac cgatcacggg ctcgccgcgg
     1201 ccggtccgga atgaccgact cggaacggaa tcgtccgatc gcactgtctg cggctcggcg
     1261 accgacgcgg cgacggtctc ccagaggcca tggatgacgt cgaacagcgc tgtacttcgg
     1321 ttcggcctgg tcatggtcct ttcggagcag cgcctggatg atgcgaggtc cgccgacgag
     1381 cgatccgatc aggcactgcg aggaacaact ggagcttgtc cacgataggc aaaaatcgca
     1441 agccaaagct tcgcacattg cgatctcacc acgtgattga cacaccgcga tctgcgacgt
     1501 gcagcaacgc ggcgcatctc tcccaatgta cgacccgcgc gatcgcgttt tctcgtcgag
     1561 caatacgccg catgtgagcg cgccggtggt gtgcgtcggg gatcgcagcg gcgcgcttcg
     1621 caccgaccgt gcggtcgtgc gcgggcgcgc gatcccgcgg tgttcctcat cggtaacctg
     1681 tgtagcggtg gactcgaaat cgtccaccgc acgcccgatc gacgccggag cgcgcggcgc
     1741 cccgcggatg cggccgacgc aggatcgcga cgatcacccg acatcccagg ctgcgcccgg
     1801 atggggcgat gcgcagcgga ccactgtgcc atgggcgcgc tgtcgtcgat cccacggggg
     1861 gctctgcccc ccgtctcgct tcgctcgagg cggcgcgctc ggccggccgc gctcgtcgcg
     1921 gtgaccccgc ggcgccggcg accgtgtcgg gaacgctcac tgtccggatc gcggacatcc
     1981 tggtcgagcg gatgccgatc cggcgcgccc cgcgtcgtgc cgcgccgcca cgacggctca
     2041 gccgatcgtg tggcggatca ccaggtaggc ataggcgtac ggtgcgatga acagcaccgc
     2101 gtcgatccgg tcgagcaggc cgccgtggcc ggggagcagc gcaccggagt ccttgacgcc
     2161 gaccgagcgc ttgatcagcg actcggtgag atcgcccagc tggccgagga caccgcccgg
     2221 gatcgcgagc agcacgacgt cgagccagct cagccagtgc gccgagatca gcttgagcgc
     2281 gacgacgccg gccagcgagc cggcgaggcc gccccacgcg ccggcccacg tcttcttggg
     2341 gctgaccgct tcgtagagcc gcgccttgcc gaggaaccgg ccggcgaagt aggcgccggt
     2401 gtcggcgacc caggcgacga tcagcacgat cagcaccgca tcgccgccct cgccgggctg
     2461 gaaccgctcg atcagcttga gcttggcgag gtaggtcagc aggtatccgg cgtagacgat
     2521 gccggtcacg gtcgcggcgt agcggccggc gaccgagggg atgtcgcgga accggaacag
     2581 gaagtacagc ccgggagcga ccacggcgag cgccagcggc accgcctggc cggtcccctc
     2641 ggcgccgacc aggacggcgg gtggcacgcc gtacccggcg agcgtcatcg gatcgagcca
     2701 gtagaacgcg gcggtggcga tcccgcccag caccagcgcg gcgcggcgat cctcggccgg
     2761 gagcagcatc gcgaacgcct cgtgcatcgc gatcagcgag gcgccgaaga tcagcgccca
     2821 ggtcggttcg ggccggtggt agtagaccag cgccagcagg atcgggacgg cgaccaccgc
     2881 aacgaggaat cgctgagcga ggttgccgag ggccacgaag agacggagct aaccgcagcg
     2941 cgcgatctcc gtcaagccca accccggcgc cgcgccggcg cacggcggat cgccgacgtc
     3001 acgacgactc gacgtccgat ccgagctggc cggtcgtgag gccgaaccgt cgctcgcggg
     3061 cggcgtactg gtccagacat gcgtagaggt gctcccggcg gaactccggc cacagcacgt
     3121 ccacgaacac cagctcggcg taggcgatct cccacagcat gaagttcgag atccgctgct
     3181 cgccgctcga tcggatcagc aggtcgagcg gcggcagctc cgcggtcggc aggtagccgc
     3241 tgaaccgctc gacgtcgacc tggtcgggcg acagccggcc gagcaccgca tcgcgcaccg
     3301 ccgcgcggac cgcccgcgcg atcgactccc ggccgccgta cgacagcgcg agcgtgaggg
     3361 tcatgccgtg atttgcggcg gacgctgccc ggagctcgtc gagcggctcc cggaccatcg
     3421 gcggcagccg ctcgacgtcg ccgatcgcct cgagccggat cgcgttgtcg aggatctcgg
     3481 cgcgctcgcc gagcaggtag tcgcgcagca gctgcatcag cccgtggacc tcgtcgaccg
     3541 ggcgctgcca gttctgcgac gagaacgcgt acagcgtgag cgcctcgatg ccgagctcgc
     3601 gcgccgcgcg ggtgacgtcg cggaccgagt cggcgccgac gcggtggccg tcgagccgcg
     3661 gcagtccgcg acgctcggcc caccggccgt tgccgtccat gatgatgccg acgtgacgcg
     3721 gcagcttgcg gggctcggtg gacatcgtgt ttcgcggatc gtgtggatcg cgaagcaccg
     3781 cgagtagcac gctcggagcg agcgttgcaa ccacaccttt gatcccgtac gctcggtgga
     3841 agccatgggg atcttttcca gactcgggac gctgatcaag tccaacatca acgatctcat
     3901 cacgaaggct gaggatcccg aga
//
LOCUS       DASVOR010000181         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_440039,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000181 DASVOR010000000
VERSION     DASVOR010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440039"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VF516_04065"
     CDS             <1..189
                     /locus_tag="VF516_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106876.1"
                     /translation="DDERCRDHARRIAVKEARRIAGCDLVAHSVDLESRARGRELHLS
                     LNVEAQRERRAQPPGTAR"
     gene            242..1177
                     /locus_tag="VF516_04070"
     CDS             242..1177
                     /locus_tag="VF516_04070"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8106877.1"
                     /translation="MIELYSIRDVSRILAVQESRLRYWMQTGFVGPTVRKGGRFYYTF
                     PDLVAVKAAKDLLATGMPLQRVRKNVEALKRALPGDTHPTTRLRVCCDGETVVALADD
                     VAFQPIGGQVVMAFSLPSFGEHVTATLAMPRAAAPEPAPPGVPASVEDGPTEAHGGST
                     AYRQFVEACGAEDRGDSDTAEHLFRLAIELEPRMAAALTNLGNLVYRQGELHEARRLY
                     ERALEHDPAQPEARYNLANLLEDLGETDLAIAELRRVCAAAPEFADAHYNLGIMLAQV
                     GGTTQARQCLERYLELDATSDWAHHARSYLDQIAV"
     gene            complement(1368..>2395)
                     /locus_tag="VF516_04075"
     CDS             complement(1368..>2395)
                     /locus_tag="VF516_04075"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8106878.1"
                     /translation="LHLSDLHFGANDQAIVSYSQLAADLHAQGVDRLDALIVSGDLVN
                     RADPAEYVAARRFLDLLMSGFALSPEQIVLVPGNHDVSWPVSEDAYRPWRRSRYPGTL
                     APGSYIDHEGGLIEVVDQDGHRQRFRPFADLYRATKGTEYSLAYEHQATVHQLANAGI
                     CIVGLNSAWEIDHHFRDRASIHPEALANALLEVGKPVAGQLRIAVFHHPLHGGEDSRI
                     RDTGFLQQLAVHGFRLVLHGHVHKADSEVYRYDRTEDGRRIEIVAAGTFGAATREWVP
                     GYPLEYNLLVVTPDKVTVETRCRREIHGAWEADARWRQGPGKDPLPRYVIDRNDMAAS
                     PRASSHP"
BASE COUNT          363 a          876 c          850 g          306 t
ORIGIN      
        1 gacgacgagc gctgccgcga ccacgcccgg cggatcgcgg tcaaggaagc gcgccggatc
       61 gccggctgcg acctggtcgc gcacagcgtc gacctggaga gccgggcgcg cggccgcgag
      121 ctccacctca gcctcaacgt cgaggcccag cgcgagcggc gcgcccagcc gcccggcacc
      181 gcgcgctgac ccccttcctc ccgagccggc ccagccccca cgaggagatc ccgaggagac
      241 catgatcgag ctgtactcca tccgcgacgt gtctcgcatc ctcgccgtcc aggagtcgcg
      301 cctgcgctac tggatgcaga ccggcttcgt cggcccgacc gtgcgcaagg gcggccggtt
      361 ctactacacg ttccccgacc tggtcgcggt caaggccgcc aaggacctgc tcgcgaccgg
      421 catgccgctg cagcgcgtgc gcaagaacgt cgaggcgctg aagcgcgccc tgcccggcga
      481 cacccacccg acgacccggc tgcgggtgtg ctgcgacggc gagaccgtgg tcgcgctcgc
      541 cgacgacgtc gcgttccagc cgatcggcgg ccaggtcgtg atggcgttca gcctgccgag
      601 cttcggcgag cacgtcaccg cgaccctggc gatgccgcgc gccgccgcgc ccgagcccgc
      661 gccgcccggc gtgccggcca gcgtcgagga cggcccgacc gaggcccacg gtggcagcac
      721 cgcctaccgg cagttcgtcg aggcgtgcgg cgccgaggac cgcggtgact cggacaccgc
      781 cgagcacctg ttccggctcg cgatcgagct cgagccccgg atggccgccg cgctcaccaa
      841 ccttggcaac ctggtgtatc gccagggcga gctccacgag gcgcggcggc tgtacgagcg
      901 tgcgctggag cacgacccgg cccagcccga ggcccgctac aacctcgcca acctgctcga
      961 ggacctcggc gagaccgacc tcgcgatcgc cgagctgcgc cgggtgtgcg ccgcggcccc
     1021 cgagttcgcc gacgcgcact acaacctcgg catcatgctc gcccaggtcg ggggcacgac
     1081 ccaggcccgg cagtgcctgg agcgctacct ggagctcgac gcgacgagcg actgggccca
     1141 ccacgcgcgc agctacctcg accagatcgc ggtgtgaacg ccgcggctag cccggataat
     1201 tcaccacgac gtcgagcgag accaagcggc gcgaggcaga tcggggcgca cccccggaag
     1261 cgcagcgagg atagtactgg acgtacttga cgagcgagaa ccggaggtac gccacgagat
     1321 gcccgcgccg cgacggtcgc agccggcggc gtggtgaatg atccgggcta ggggtgcgat
     1381 gacgcgcgcg gactggccgc catgtcgttg cggtcgatga cgtagcgcgg cagcgggtcc
     1441 ttgcccgggc cctggcgcca gcgcgcatcg gcctcccacg cgccgtggat ctcgcgccgg
     1501 cagcgcgtct cgacggtgac cttgtccggc gtgacgacca gcaggttgta ctccagtgga
     1561 tagcccggga cccactcgcg ggtcgccgca ccgaacgtgc cggcggcgac gatctcgatc
     1621 cgccggccgt cctcggtgcg gtcgtagcga tagacctcgg agtccgcctt gtggacgtgg
     1681 ccgtgcagca cgagccggaa cccgtgcacc gcgagctgct ggaggaaccc ggtgtcgcgg
     1741 atccgcgagt cctcgccgcc gtgcagcggg tggtgaaaca cggcgatccg cagctgaccg
     1801 gcgaccggct tgccgacctc gagcagcgcg ttggccagcg cctcgggatg gatgctcgcg
     1861 cgatcgcgga agtggtggtc gatctcccac gccgagttca ggccgacgat gcagatcccg
     1921 gcgttggcga gctggtgcac ggtggcctgg tgctcgtagg ccagcgagta ctcggtgccc
     1981 ttcgtggcgc ggtacagatc ggcgaacggg cggaaccgct ggcggtggcc gtcctgatcg
     2041 acgacctcga tcaggccgcc ctcgtggtcg atgtagctgc ccggcgcgag cgtgccgggg
     2101 tagcgcgacc ggcgccacgg ccggtacgcg tcctcggaca ccggccagct cacgtcgtgg
     2161 ttgccgggca ccagcacgat ctgctcgggc gacagcgcga accccgacat cagcaggtcg
     2221 aggaaccgcc gcgccgcgac gtactcggcc ggatcggcgc ggttgaccag gtcgcccgac
     2281 acgatcagcg cgtcgagccg atcgaccccc tgggcgtgga gatccgccgc gagctgggag
     2341 tacgagacga tcgcctgatc gttggccccg aagtgcagat ccgacaggtg cagca
//
LOCUS       DASVOR010000182         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_235475,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000182 DASVOR010000000
VERSION     DASVOR010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235475"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VF516_04080"
     CDS             complement(<1..242)
                     /locus_tag="VF516_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106879.1"
                     /translation="MRPGRSISASISAMWAARAAWGPRAAVAAALLAWSFWQRWQLLT
                     ASPFPLGVDGYFYPVQLRALLARGELAYPASPLAFYL"
     gene            758..1447
                     /gene="queC"
                     /locus_tag="VF516_04085"
     CDS             758..1447
                     /gene="queC"
                     /locus_tag="VF516_04085"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: protein motif:HMM:NF018242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEX8106880.1"
                     /translation="MSAAVVLLSGGLDSTTALAVARSRGFTCHALTVRYGQLHDVELA
                     AAARVAAALGAAAHHVVEVGLAPIAASALTTAGIAVPKDRSLDEIGAPGDVPATYVPA
                     RNTVLLALALAWAESLGARDLFIGVNVLDASGYPDCRPAFVHAFEALAQVATRAGGYT
                     VHAPLIDLTKAQIIALGQSLGVDYALTHSCYDPQGGAACGRCDACALRRKGFAEAGVP
                     DPTRYAPDAPR"
     gene            1516..2622
                     /locus_tag="VF516_04090"
     CDS             1516..2622
                     /locus_tag="VF516_04090"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106881.1"
                     /translation="MKLGTLLLRNAAIGLTQLEAALRNQVLYGGRLGTNLVELGYIDL
                     ELLSAYLAELSGLPIATPTLLDQADAGLIEQLGAEDAHRLRAIPLGYLGEGTGTVAVA
                     LVEPGDSRVVEQVAGKLGAQVTPYVVPELRALYYLETLFGLPRRARFVRAGRSGAGNG
                     DLPGERRRTQPAMGMVMPPALTLEPRRRRASSPAPLVGVVPSAISFAIACERIDTANN
                     RDQIAETFAAYAPGRCDALVVLLIRDGNALGWRAWVAPPGALRKPIEELSLPLGGASS
                     LQSAHDAGQPFVGTPPSAARPIEKQLWAALGTQPEPFAVLVVPVLVKQRAVNLVYAHI
                     VSSPPPNQLVTELGELAARAQTSYLRLIRQARGS"
BASE COUNT          426 a         1072 c         1073 g          435 t
ORIGIN      
        1 aggtagaacg ccagcggcga cgccgggtag gcgagctcgc cgcgcgccag cagcgcgcgc
       61 agctggaccg gatagaagta gccgtcgacg ccgagcggga acggcgacgc ggtgagcagc
      121 tgccagcgct gccagaacga ccacgcgagc agcgccgcgg cgacggccgc ccgggggccc
      181 cacgccgccc gcgctgccca catcgccgag atcgaggccg agatcgagcg cccgggacgc
      241 accgggtgga gtctactacc gacgccgcgg gcgttcgccg tccggagttc cgcgagggca
      301 cggcgacgag gcgatggggc ctgtggcgac ggtcgatccc cgagcgctcc cgacggtggg
      361 gtcaaggtcg acgtctgatg attcgtcaca agtcctcgac ggcgttcgtg atcgtcgacg
      421 tcgacctgat cgttgaccca gagcgttgag gtcgacggcg acgtacgacg ttgagcccac
      481 catcgacgtt ggacgtcgac cgatcctgtg ctgcgctcct cagcgagggc gacaacgggg
      541 gcgaggtcga ggtcgaggtc gaggtcgagg gtggcgtcag acgtctacgt atcgcttcgc
      601 tgccgtctac ctcaacggtg gggtcaaggt cgacgttcag gtcaacccca tgtcccccaa
      661 gttgtggcgg atcatcagag cgttcccgag cgctccgggc gcgggatccg gggccctgga
      721 tccgcgaaag cggtcgtccg ggcgctacag tggcggtgtg tcagctgcag ttgtcctgct
      781 ctccggcggc ctcgattcga cgaccgcgct cgcggtcgcg cgatcccgcg gcttcacgtg
      841 ccatgcgctg accgtgcgct acggccagct ccacgacgtc gagctcgccg cggcggcgcg
      901 ggtcgcggcg gcgctcgggg cggccgctca ccacgtggtc gaggtcgggc tcgctccgat
      961 cgcggcgtcg gcgctgacca ccgccgggat cgccgtgccc aaggaccgct cgctcgacga
     1021 gatcggcgcg ccgggcgacg tccccgcgac gtacgtgccg gcccgcaaca ccgtgctgct
     1081 cgcgctggcg ctcgcgtggg ccgagtcgct cggcgcccgc gatctgttca tcggggtcaa
     1141 cgtgctcgac gccagcgggt accccgactg ccggccggcg ttcgtccacg cgttcgaggc
     1201 gctggcccag gtggcgaccc gggccggcgg ctacaccgtg cacgccccgc tgatcgacct
     1261 gaccaaggcg cagatcatcg cgctcggtca gtcgctcggc gtcgactacg cgctgaccca
     1321 ctcgtgctac gacccgcagg gcggcgccgc ctgcgggcgg tgcgatgcgt gcgcgctgcg
     1381 gcgcaagggg ttcgccgagg ccggcgttcc ggatcccacg cgctacgcgc cggacgcgcc
     1441 acggtgacgc gccaggacgg tgacgccggg ctgcggcgat cgcgcgtccc gggtcaacca
     1501 ggttaagatg ccgtcgtgaa gctcggcacg ctgctcctgc gcaacgccgc gatcgggctg
     1561 acgcagctgg aggccgcgct gcgcaaccag gtgctgtacg gtgggcggct cgggaccaac
     1621 ctggtcgagc tcggctacat cgacctggag ctcctgtcgg cgtatctcgc agagctcagc
     1681 ggcctgccga tcgccacgcc gaccctgctc gaccaggccg acgcggggct gatcgagcag
     1741 ctcggcgccg aggacgcgca tcgcctgcgc gcgatcccgc tgggctacct cggggagggc
     1801 accggcacgg tggcggtggc gctggtcgag cccggcgatt cccgggtcgt cgagcaggtc
     1861 gccgggaagc tgggcgccca ggtcacaccg tacgtcgtcc ccgagctgcg agcgctgtat
     1921 tacctggaga cgctgttcgg gctgccgcgc cgggcccggt tcgtccgcgc cggccggtcc
     1981 ggggccggca acggcgacct gccgggcgag cgccggcgca cgcagcccgc gatgggcatg
     2041 gtgatgccac ccgccctcac gctcgagccc cggcgccggc gggcgtcgtc ccccgcgccg
     2101 ctggtcggcg tcgtgccgtc ggcgatcagc tttgccatcg cgtgtgagcg gatcgacacc
     2161 gcgaacaacc gcgaccagat cgccgagacg ttcgccgcct acgccccggg tcggtgtgac
     2221 gcgctggtcg tgctgctgat ccgcgacggc aacgccctgg gctggcgcgc atgggttgcg
     2281 ccgccgggcg cgctgcgcaa gccgatcgag gagctgtcgc tgccgctcgg gggcgcgtcc
     2341 tcgctgcagt cggcgcatga cgccggccag ccgttcgtcg ggacgccgcc gtccgcggcg
     2401 cgccccatcg agaagcagct gtgggcggcg ctcgggaccc agcccgagcc gttcgcggtg
     2461 ctcgtcgtcc cggtgctggt caagcagcgc gcggtcaacc tcgtgtacgc ccacatcgtc
     2521 agcagcccgc caccgaacca gctggtcacc gagctcggcg agctcgccgc gcgggcgcag
     2581 acgtcttact tgcgtctgat tcgccaggcg cgcggctcct gatcgccggc cgctgtgcca
     2641 cttccgccac agtcggagcg ggagctcggc ccggcacccc ccggcaatct tgcccgcggg
     2701 tcaagcgttc cagagcaaaa attaggacgc gcgatcagcc ctaacgattt gatatgaata
     2761 atgaagttac gaggatctca aggccgggcc cggaacttga ctccgcccgc caaagaggga
     2821 aagtgccatc tgtgccacaa caaatctcca tgccgaacca gggctgcaag gtcgggctcg
     2881 gcacgatcgc tgcggcgtta gccgctctct cgatggctgt gctgggacac ttcttcatct
     2941 agcgaactcg tagcgaaccc gacctcacga acctctcggg atacacccga ggccacggat
     3001 gccgcg
//
LOCUS       DASVOR010000183         4960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_557054,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000183 DASVOR010000000
VERSION     DASVOR010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557054"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..604)
                     /locus_tag="VF516_04095"
     CDS             complement(26..604)
                     /locus_tag="VF516_04095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106882.1"
                     /translation="MIDLQREVNRTVQGFVAQIVELVHHTAVETLQTAFVGAGAAERS
                     GERGPIAAGTSAKRTQADLQALSQRLAAFVQANPGLRIAQINQALGTKTKDLMLPIRK
                     LTASGVIRTTGEKRTTRYFAIPGPALEQELAPRPEPEADAPRIEPPRDPPTRPAGEPV
                     VVRAGASAPSEPDAGWQRCLADVFTLAQRLAK"
     gene            complement(645..1910)
                     /locus_tag="VF516_04100"
     CDS             complement(645..1910)
                     /locus_tag="VF516_04100"
                     /inference="COORDINATES: protein motif:HMM:NF033519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAzo13 family transposase"
                     /protein_id="HEX8106883.1"
                     /translation="MCGLTSRIRLGRMSRNNLFREMPIARIRAKFLALKPVLDERLTR
                     LWAGAEAEAAGDGGVTIVARATGMSRTTIRAGRDELRKGLTASDVVRVRRAGAGRRSI
                     EDKVPGIVGALEGLIEPTTRGDRESPLRWTLKSTRALSAELGRRGFSISPQKVGQLLH
                     ARGYSLRGGGALPVTTPPAVRHRQFELINRRVEAFQARNAPVIAVDTRYTALAGDVSH
                     GECAWQRGDEPAVAPRRGAIEAGLGRPVAHDASDGACHAGRIDADVDDKMPAFALLAI
                     TEWWEHMARRGGLHATELLVMIDRAGGGASAQRWRSELQRFADRSGLAVGVSHVPPGT
                     SKWHRVEHRLLCHVTESWHGHARAEREVAIQLIGSARTVHRPVALAAFRGQAREIEML
                     EDSSSLLITRDGVHGEWNYTLHPHRERPQ"
     gene            2383..3708
                     /locus_tag="VF516_04105"
     CDS             2383..3708
                     /locus_tag="VF516_04105"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8106884.1"
                     /translation="MKLRHLAPLSLIPASLVIASCSAPGSSAPDPAAQDPGVAVATQA
                     DDPAPGPHVNAVTEAPTGFDGLNNGNCTQQAMFDAHDTFSEVEAFEDGIGPTFNNTSC
                     ISCHEDPRGTAENATNLKAFGGTGSQITELRAGHFNGISFVDHPGGSLINDRANDRSI
                     QEHILSGNEVQTLRLTISLAGDGFVEAIDSNTLLAISNAQPAAQRGTLIQVPVLEAAG
                     QNRAGRFGWKDQHSSLLSFASDAYVNEMGVTNRFAQTENTSNGTVVQGGTFDGKADPA
                     GTGEDDAGDIDAFTTFMRCLKAPPRGPITSAVTAGQTTFNSIGCAVCHVGTITTAPAG
                     TVINGGALTVSTALGDKNIHPFGDFLLHDVGTGDGIVQNGGQGTRNQVRTAPLWGIGS
                     RTRFMHDGASLTATAAIQRHATQAATAKTNFNALSATDQSNVLAFIFSL"
     gene            complement(4086..4622)
                     /locus_tag="VF516_04110"
     CDS             complement(4086..4622)
                     /locus_tag="VF516_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106885.1"
                     /translation="MPIVEGGRTTHTTLECKSVVDSLRFYRDVMGLRVNQIAPGVGHV
                     FDSKGQYAAALQNANPGPQPFLNSYARPVADAAAVDAIHARISAIRDEHRIAEITAPA
                     REDPARFGVASYGFYQEGVLGDPPARVVLVLPAGSGHQLVGARDLARRDQPGRPGSGA
                     GAAVVGVTGRGALADEAR"
     gene            complement(4657..>4960)
                     /locus_tag="VF516_04115"
     CDS             complement(4657..>4960)
                     /locus_tag="VF516_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106886.1"
                     /translation="VGDTRFKTPLSWLFALRELDATIELPAGLTLALTYDHGLLRASG
                     AVFGATPVTVPVALPSAQPGDDARTEIARLAAFGKDVAQRLVLQRAADWLESQLSP"
BASE COUNT          789 a         1771 c         1593 g          807 t
ORIGIN      
        1 gtagagcagc tcgcgtcgcc cgccttcact tcgccagtcg ctgggccagc gtgaagacat
       61 ccgccaggca acgctgccag ccggcgtcgg gctcgctcgg tgcactcgca ccggcgcgga
      121 cgacgaccgg ctcgccggcg ggccgggtcg gtggatcacg cggaggctcg atacgcggcg
      181 cgtcggcctc gggctctggc ctcggtgcaa gctcctgttc gagcgccggg cccgggatcg
      241 cgaagtacct cgtcgtgcgc ttctcgcccg tggtgcggat cacgccgctg gccgtcagct
      301 tgcggatcgg cagcatgagg tccttggtct tcgtacccag ggcttgattg atctgcgcga
      361 tgcgcaggcc agggttcgcc tggacgaacg ctgcgaggcg ctgcgacagc gcctgcaagt
      421 ctgcctgggt gcgcttggcg ctcgtgccgg ccgcgatcgg tccgcgttca ccggatcgct
      481 ccgcggcgcc agcgccgacg aacgcggtct gcagcgtctc gacggcggtg tgatgtacga
      541 gctcgacgat ctgcgccacg aacccctgta cggtgcgatt cacctctctc tggaggtcga
      601 tcatgtgcgt ccttctggat ggccgatgag ctgcggtcgg ccggtcactg cggtcgctcg
      661 cgatgaggat ggagggtgta gttccactca ccgtgcaccc catcccgggt gatgaggaga
      721 ctgctgctgt cctcaagcat ttcgatttcg cgcgcttgcc cgcggaatgc agccagcgcc
      781 acgggacggt ggacggtgcg cgcgctgccg atcagctgga ttgcgacctc gcgctcggct
      841 cgagcgtggc cgtgccagct ctcggtcacg tggcagagca gccgatgctc gacgcggtgc
      901 cacttcgagg tgccgggagg cacgtgcgag acacccacgg cgagcccgct gcgatccgcg
      961 aaccgctgca gttcggaccg ccagcgttgc gcggacgcgc ctcctcccgc tctgtcgatc
     1021 atgaccagca gctccgttgc gtgcagcccc ccgcgccttg ccatgtgttc ccaccattcg
     1081 gtgatcgcga gcaacgcgaa tgcgggcatc ttgtcgtcga cgtcggcatc gatccggccg
     1141 gcgtggcaag ctccgtcgga tgcgtcgtgc gcgacgggcc taccgaggcc ggcctcgatc
     1201 gcgccacgcc tcggcgcgac cgccggttcg tctccccgct gccacgcgca ctcgccatga
     1261 gagacatcgc ctgcgagcgc cgtgtaccgg gtgtcgaccg cgatcaccgg cgcgttgcgc
     1321 gcctggaacg cctcgacccg ccggttgatc agctcgaact gccggtggcg gaccgccggc
     1381 ggagtggtca ccggcagcgc tccgccacca cgcaggctgt aaccgcgcgc gtgcaggagc
     1441 tggccgacct tctgcgggct gatcgagaac ccacgccgac ccagctcggc ggacagcgca
     1501 cgcgtgctct tcagggtcca gcgcagcggc gattctcggt cgcctcgcgt cgtcggctcg
     1561 atcagccctt cgagcgcacc cacgatcccc ggcaccttgt cctcgatcga tcgtcgccct
     1621 gcgccggcgc ggcgcactcg caccacgtcg ctcgcagtca gccccttgcg aagctcgtct
     1681 cgtccggcgc ggatcgtcgt ccgggacatt ccggtggctc gcgccacgat cgtgaccccg
     1741 ccgtcaccgg ccgcctcggc ctcggcccct gcccacagcc gcgtcaagcg ctcatccagc
     1801 accggcttga gcgccaggaa cttcgcccgt atgcgtgcta tcggcatctc cctgaacaag
     1861 ttatttctgc tcattcggcc caatcggatt ctcgacgtga gtccacacat agcatgggtt
     1921 tctgtggact agttattctt ggccagctcg accgattgtc cgatggattg cggacgaaca
     1981 aaggggcaaa atgcccccga tctcctcaga gaaagtgacg agaaactgag gcagcggtcc
     2041 ctccgatcag tctctcgact tcaaaaaact gactgcgcgg cacgagcaag caacgtgagc
     2101 cacaacaggc tcgggagtga gtttaaagat tctcttcacg atcaaaattg tgacctctca
     2161 tttaatcgaa tcgattcgaa attcgattga cgatcagccg tccagccgac gtagagtgtt
     2221 tcacataaag gcggggtcca agatgagcgg cgcgctcctg cgctgcagat cgaccaccag
     2281 aaacgaagac gacctcgcgt ccccgtcacc cgtgtccatg gggtgtaccc gcaccccaca
     2341 tgactctcgc tccgtttgct gaaccccagg agactcacag ccatgaaact tcgccatctc
     2401 gctcctctgt cattgatacc ggcgtcgctg gtcatcgcgt cgtgctccgc gccgggcagc
     2461 agtgcgccgg accccgcagc gcaggatcct ggcgtcgcgg tcgcgaccca ggccgacgac
     2521 cccgcgcccg gcccgcacgt caacgcggtc accgaggccc cgaccggctt cgacggcctc
     2581 aacaacggaa actgcaccca gcaagcgatg tttgatgcgc acgacacgtt ctcggaggtc
     2641 gaggcgttcg aggacggcat cggcccgacg ttcaacaaca cctcgtgcat cagctgccac
     2701 gaggacccgc gcgggaccgc ggaaaacgcc accaacctca aggcctttgg cggcacaggc
     2761 agccagatca ccgagcttcg cgccggtcac ttcaacggca tcagcttcgt cgaccacccg
     2821 ggcggctcgc tgatcaacga ccgcgcgaac gaccgctcga tccaggagca catcctgtcg
     2881 ggcaacgagg tccagacgct ccggctcacg atcagcctcg cgggtgatgg cttcgtcgag
     2941 gcgatcgaca gcaacacgct gctcgcgatc tccaacgccc agccggccgc ccagcgcggc
     3001 acgctgatcc aggtcccggt gctcgaggcg gcgggccaga accgcgccgg ccggttcggc
     3061 tggaaagacc agcactccag cctgctgtcg ttcgcctcgg acgcgtacgt gaacgagatg
     3121 ggtgtcacca acaggttcgc gcagaccgag aacacgtcca acggcaccgt ggtgcagggc
     3181 ggcacgttcg acggcaaggc cgatccggcc ggcaccggcg aggatgacgc gggcgacatc
     3241 gacgcgttca cgacgttcat gcgctgcttg aaggcgccgc cgcgcggccc gatcacctcc
     3301 gccgtgaccg cgggtcagac gacgttcaac agcatcggct gcgcggtctg ccacgtcggc
     3361 accatcacca cagctccggc gggcaccgtg atcaacggcg gcgcgctcac cgtgtccacc
     3421 gcgctcggcg acaagaacat ccacccgttc ggcgacttcc tgctccacga cgtcggcacc
     3481 ggcgacggca tcgtccagaa cggcggccag ggcacgcgca accaggtccg caccgcgccg
     3541 ctgtggggca tcgggtcgcg cacccggttc atgcacgacg gcgcgagcct gacggccacc
     3601 gcggcgatcc agcgccacgc cacccaggcc gcgaccgcga agaccaactt caacgcgctg
     3661 tccgcgaccg accagtccaa cgtcctggcg ttcatcttct cgctgtagcc agcgcccggg
     3721 ccggagtgct cgaccgatgt aatgccgcgt cgcatgtacg gtcgagccac cctcgaattc
     3781 tcgatgggcg ataccgcgct agatcgtgca attctgacgc acgacatgta tgcatgtgcg
     3841 tttgtttcca ggcgccccgg cgcttgtgcg ggtccgcggc gccgggcatg ttggtacgat
     3901 gcgtgcgcga cgcgcttcct gtcgtgagtc gaaaggtgca gtgacccacg cgtacgactc
     3961 gagctgggcg gcgatcgcca tcgtcgtgac ggccgccgtc gcgttcgcag tgggcgcatt
     4021 ctgctggttc atcgactgac gagcgactgc gcgcgtcgac tcgcgcggtc accgtgactt
     4081 cagcgtcacc tcgcctcgtc ggcgagcgcg cctcgccccg tcaccccgac gacggccgcg
     4141 cccgcgccag accctggtcg accgggctga tcgcgtcgtg ccagatctcg agctcccacc
     4201 agttggtgtc cggatcctgc aggtagaacg agtacgaccc gtgctggtgg gtcgccgaga
     4261 acaccttctt ggtagaaccc gtagctggcg accccgaacc gcgccggatc ctcgcgggcg
     4321 ggcgcggtga tctcggcgat gcggtgctcg tcgcggatcg ccgagatccg ggcgtggatc
     4381 gcgtccaccg cggcggcgtc cgcgaccggc cgcgcgtagg agttgaggaa cggctgcggg
     4441 ccgggattgg cattctgcag cgccgccgca tactggccct tgctgtcgaa cacgtgcccg
     4501 accccgggcg cgatctggtt gactcgcagg cccatcacat cgcggtagaa ccgcaggctg
     4561 tccacgacgc tcttgcactc cagcgtggtg tgcgtggtcc gtccgccctc gacgatcggc
     4621 atgccccacc gtagcaccgt tgcggccgtc tcgacttcac ggcgacagct gcgattccag
     4681 ccagtccgcg gcgcgctgca gcaccaggcg ctgcgcgacg tccttgccga acgcggccag
     4741 ccgggcgatc tcggtgcgtg cgtcatcgcc gggctgcgcg gacggcagcg cgaccggcac
     4801 ggtgaccggc gtcgcaccga acaccgcgcc ggacgcgcgc aagaggccgt ggtcgtacgt
     4861 gagcgccagc gtgagcccgg cgggcagctc gatcgtcgcg tcgagctcgc gcagcgcgaa
     4921 cagccacgac agcggcgtct tgaaccgcgt gtcgccgacc
//
LOCUS       DASVOR010000184         6892 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_1825,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000184 DASVOR010000000
VERSION     DASVOR010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6892
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1825"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VF516_04120"
     CDS             <1..280
                     /locus_tag="VF516_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106887.1"
                     /translation="ADPGGGGRLAPRLLVGAGAAALVAGGVLYAIDQDPGGPSPVSRN
                     TAPAGIAVGAVGLAAVSVGLWLWGARGGSSPMLAIGSSGGFIGWGGAL"
     gene            346..1368
                     /locus_tag="VF516_04125"
     CDS             346..1368
                     /locus_tag="VF516_04125"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8106888.1"
                     /translation="MRLREWGTDLIRVLPAPPVSGWMVGASTSCALRLDDPSGQVSRV
                     HASLIHDQARWRLRDAGSMNGMRLDGARRTEIILEPGIEIGIGGLTLIAESRRWIALR
                     GFLARLLGWRGDRTEVVDRALRSIRMAATHRTALVLCGDGDLVPTARSIHRHALGDDR
                     PFIVCDPRRRRSKATVRSAENYETGMQAFEAAHGGSLCVRNRRLPRDFEQVAAALRDS
                     HARVQLLVCAGRSDDCRSYLASPITVPSLAGREAELDRIIDEYAEDAVAELGAPRVGF
                     LTVDHAWVREHAASSLSEIEKATLRLVALRHSRNLSHAAARLGMAPVSLSRWIGRREM
                     PMPVVP"
     gene            1579..1797
                     /locus_tag="VF516_04130"
     CDS             1579..1797
                     /locus_tag="VF516_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106889.1"
                     /translation="MVRSDLTFNGVKVFSATMFAARDQLGETVTAWLAANPHLLVTDF
                     VVTQSSDSQFHCVAITVFYWEKLHSSRG"
     gene            2019..3692
                     /locus_tag="VF516_04135"
     CDS             2019..3692
                     /locus_tag="VF516_04135"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106890.1"
                     /translation="MGASDLLAGVTTRLASTARLDEIVDTVLNEIVGLGFGAVWVVRL
                     DEPSGELVTLKSVVDGVDITARVPRISVDSRHPVSYGFRERRMVNVTDPDSLHILERE
                     DEAVPPGRMALSRGVYDHLRGHPFACGPLFGSRGQPVGALGVSSYRGQQPIPDELLTQ
                     GVFRTFMDHLGLAMERAVHVEQLQASLVKAQAAIVNDARIKAVGELASAVAHDLNNLT
                     GIALLAASVGMRSPADAVDMLPRIERANRAIGDLVARLQRLARAPSGEPDAANLQQIL
                     EDILIIVKPVLRERSIEVEAVLPAAVPAVRCDPVLVHQVVLNLVMNAHDALDAVPPDQ
                     RRIRIELRDDDGVVRVTVRDTGPGIAPEVLAQLFQPFITTKRDAHLGLGLAAARAALQ
                     HFGANIEGRNAPSGGAVFEVSLVAAPPGTRTAKPLHPPRPKDQPSPRRARILAIDDDP
                     DVVYIICAYLEPHGHAVATATSSAEALEIARAQPFDLVLCDIGMPKQNGIDVCRSLRD
                     AGYRGKLVLMTGWENYELSAEQRAVSFESVIKKPFLGADLLAVIDNVLG"
     gene            complement(3689..4057)
                     /locus_tag="VF516_04140"
     CDS             complement(3689..4057)
                     /locus_tag="VF516_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106891.1"
                     /translation="MARCILVVDDDRDIRDSLVELLDEHGYPSIGAGNGVEALEILRT
                     SASPPSLILLDLMMPVMDGREFRERQLENPAWRAIPVIVISAYNDVDRQARALALDHL
                     RKPLAIRPLIDAIRRHVAVS"
     gene            complement(4059..6443)
                     /locus_tag="VF516_04145"
     CDS             complement(4059..6443)
                     /locus_tag="VF516_04145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106892.1"
                     /translation="MSPVTANPSYRVLPESTGDLAELLDDAPVAIHRIDAAGTIVWAN
                     RAELDLLGYGAEDYIGRPLSAFCVDPDVAAGLLDRLRRGETVRDCEVRLRAHDGAIRH
                     VLISESMRLDGRGCGVSRGFLRDVTADRRAERERTQLIEELTRTVRLNDMFAGIVGHD
                     LRGPLSTIVMAGQLLLGHVDQPKPVRTIERLLNSADRMQRMISQLLDFARARADGGIE
                     LDRQPLEVPDIARDVIEEVRLARPDWKIELVVHGDVCGHHDGNRLSQVFSNLIGNAVQ
                     HGSTDAPLTVLIDGRDRDAIRVEVKNRGTIPPEVLPILFSPFRGSQQKALRSQGLGLG
                     LFITAQIVQAHGGSIAAESVDGWTAFRFDLPRRTTGARIATFDAELPAGKSGPIPIVR
                     DAAAPAPTQASIDATPEATTGSTRDAAAGDAREATRQHEERFRVLVEAIKEYAIFMLD
                     ADGKVATWNVGAQRINGYAAHEIIGRHFSVFYSDDEVRAGKCEYELRAAARDGRFEDE
                     GWRLRKDGTRLWANVVITALRDPSGALIGYAKVTRDLTERRLLDEERVRIAHAEEAVR
                     LRDEFLSLASHELKTPLTVLRLQLEALRERITGDDRATLVKLERSDRAGQRLTELIDA
                     LLDVSRIATGRFTLHLEQADLADIVATAVDRLREVAANAGCTLSVTADHAIGTWDRSR
                     IDQIVTNLVSNAIRYAAGSPITVTVERRAGDAVIEVRDQGPGLPDGQIARMFERFERG
                     TSMRHYAGLGLGLYVVRQITEAHGGQVTAENSAGGGACFTVRLPLDSTSHDLVA"
     gene            6662..>6892
                     /locus_tag="VF516_04150"
     CDS             6662..>6892
                     /locus_tag="VF516_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106893.1"
                     /translation="MGITRRILESAQSGLSRLTSLVIVDDEPLSHVESAALQAELTVR
                     KAGRERAPRLAQDNPLAKLASSDPAARAARAAA"
BASE COUNT          940 a         2408 c         2531 g         1013 t
ORIGIN      
        1 ggccgatccg gggggcggcg gtcgcctggc gccgcggctg ctggtcggcg cgggtgccgc
       61 ggcgctggtc gccggcggcg tgctgtatgc aatcgaccag gacccggggg gaccgagccc
      121 ggtctctcgc aacaccgcgc cggccggaat cgccgtcggt gcggtcggac tcgcagcagt
      181 cagcgttggc ctgtggctgt ggggcgcgcg cggcggatcc tcgccgatgc tcgcgatcgg
      241 cagctcgggc ggcttcattg gctggggagg tgctctgtga tgatgaatgg acgttcgacg
      301 aacccggatg cggcggcgag cgagtggagt atcgacgatc acgtgatgcg gctccgcgag
      361 tggggcaccg acctgatccg cgtgctgccc gcgccgccgg tgtccggctg gatggtcggc
      421 gcgtcgacga gctgcgcgct ccggctcgac gatccgtcgg gccaggtgtc ccgggtccac
      481 gccagcctga tccacgacca ggcccggtgg cggctgcgcg atgccggcag catgaacggc
      541 atgcggctcg acggtgcgcg ccgcaccgag atcatcctgg agcccggcat cgagatcggc
      601 atcggcgggc tcaccctgat cgccgagagc cggcgctgga tcgcgctgcg cgggttcctc
      661 gcccggctgc tcggctggcg cggcgaccgc accgaggtcg tcgaccgcgc gctgcggtcg
      721 atccgcatgg ccgcgaccca ccgcaccgcg ctggtgctgt gcggcgacgg cgacctggtg
      781 ccgaccgcgc ggtcgatcca tcgccacgcg ctgggcgacg accggccgtt catcgtctgc
      841 gatccgcggc gccgccgcag caaggcgacc gtgcgctcgg cggagaacta cgagaccggc
      901 atgcaggcgt tcgaggcggc gcacggcggc tcgctgtgcg tgcgcaaccg caggctgccg
      961 cgcgatttcg agcaggtggc cgcggcgctg cgcgactcgc acgcccgggt ccagctcctg
     1021 gtgtgcgccg ggcggtccga cgactgccgg tcctacctcg cgtcgccgat caccgtgccg
     1081 tcgctcgccg gccgcgaggc agagctcgac cggatcatcg acgagtacgc cgaggacgcg
     1141 gtcgccgagc tcggcgcgcc gcgggtcggc ttcctgaccg tggaccacgc ctgggtgcgc
     1201 gaacacgctg cgtcgtcgct gtccgagatc gagaaggcga cgctccggct ggtcgcgctg
     1261 cgtcactcgc gcaacctcag ccatgccgcg gcgcgcctgg gcatggcgcc ggtgtcgctg
     1321 tcgcgctgga tcggacggcg cgagatgccc atgccggtgg tgccgtgacc ggcgtacccc
     1381 tcgatcgaac cccgcggggc accggcaccc cctggaagca ctcgcagcgc ccctgtcaag
     1441 ggccaaacgc cccggatccg gacgtcgaaa tcctgcgtca cggcgcggct gaacgagctt
     1501 tgttgatgat cgcgagcgcc acgcgcgggc tctgggagta gtctgcccgc gatggcgcgc
     1561 gatgcttcag ctgggctcgt ggttcgatcg gacctgacgt tcaacggggt caaggtgttc
     1621 tcggccacca tgtttgccgc ccgggaccag ctcggcgaga ccgtgaccgc gtggctggcg
     1681 gcgaacccac acttgctcgt gaccgacttc gtggtgacgc aatcgagcga cagccagttt
     1741 cactgcgtgg cgatcacggt gttctactgg gagaagctgc actcgtcgcg cggctgacat
     1801 cgccgacctc cagcagccgt tcaccgcccc cgcgctgcgc cgcgtgctcg gcttcccccg
     1861 gcgccggaca ctcgccggtg aattcctgat gtgcagcaaa acgcgtcacc aggtttccgg
     1921 gaggaggtcc agaggactcg tatagtcgtc cgacagcgcg atccgatgcg ggtggggtga
     1981 taattgccgt tggtagcaga catatcgagg acaccacgat gggggcatcg gatctcctag
     2041 ccggagtcac gacgcgactc gcgtcgacgg cccggctcga cgagatcgtc gatacggtgc
     2101 tgaatgagat cgtggggctc gggttcggcg cggtgtgggt ggtccggctg gacgagccgt
     2161 ccggcgagct ggtgaccctg aagtcggtgg tcgacggggt cgacatcacc gcccgggtgc
     2221 cgaggatctc ggtcgactcg cgccacccgg tctcctacgg gttccgcgag cgccggatgg
     2281 tcaacgtcac cgatccggac tcgctgcaca tcctcgagcg cgaggacgag gcggtgccgc
     2341 ccggccggat ggcgctatcg cgcggggtgt acgatcacct gcgcggccac ccgttcgcgt
     2401 gcggcccgct gttcggcagc cgcggccagc cggtcggcgc gctcggcgtg tcgtcgtacc
     2461 gcggccagca accgatcccg gacgagctgc tgacgcaggg cgtgttccgc acgttcatgg
     2521 accacctcgg gctcgcgatg gagcgcgcgg tgcacgtcga gcagctccag gccagcctgg
     2581 tcaaggcgca ggcggcgatc gtcaacgacg cgcggatcaa ggcggtcggc gagctcgcca
     2641 gcgcggtcgc gcacgatctc aacaacctga ccggcatcgc gctgctcgcc gccagcgtcg
     2701 gcatgcggtc gccggccgac gcggtcgaca tgttgccgcg gatcgagcgc gccaaccgcg
     2761 cgatcggcga tctggtggcg cggctgcagc ggctggcgcg cgccccgtcg ggcgagcccg
     2821 acgccgccaa cctgcagcag atcctcgagg acatcctgat catcgtcaag ccggtgctgc
     2881 gcgagcgctc gatcgaggtc gaggcggtgc tgccggcggc ggtgcccgcg gtgcgctgcg
     2941 atccggtgct ggtccatcag gtcgtgctca acctggtgat gaacgcgcac gatgcgctgg
     3001 acgcggtgcc gcccgaccag cgccggatcc ggatcgagct gcgcgacgac gacggcgtgg
     3061 tgcgggtgac cgtccgcgac accgggcccg ggatcgctcc cgaggtgctg gcccagctgt
     3121 ttcagccgtt catcacgacc aagcgcgacg cccatctcgg gctcgggctg gccgctgcgc
     3181 gggccgcgct ccagcacttc ggcgcgaaca tcgagggccg caacgcgccg tcgggcgggg
     3241 cggtgttcga ggtcagcctg gtggcggcgc cgcccgggac ccggaccgcc aagccgctcc
     3301 acccgccgcg tccgaaggac cagccgtcgc cgcgccgcgc gcggatcctc gcgatcgacg
     3361 atgatcccga cgtggtctac atcatctgcg cctacctcga gccgcacggc cacgcggtcg
     3421 cgaccgcgac ctcctcggcg gaggcgctcg agatcgccag ggcgcagccg ttcgacctcg
     3481 tgctgtgcga catcgggatg cccaagcaga acgggatcga cgtgtgccgc tcgctgcgcg
     3541 acgccggcta tcgcggcaag ctcgtgctga tgaccggctg ggagaactac gagctcagcg
     3601 ccgagcagcg cgcggtgtcg ttcgagagcg tcatcaagaa gccgttcctc ggcgccgacc
     3661 tgcttgccgt gatcgacaac gtgctcggct aagacaccgc cacgtgccgg cggatcgcgt
     3721 cgatcagggg acggatcgcc agcggcttgc gcaggtggtc gagcgcgagc gcgcgcgcct
     3781 gccgatcgac gtcgttgtac gccgagatca cgatcaccgg gatcgcgcgc cacgccgggt
     3841 tctcgagctg gcgctcgcga aactcccggc cgtccatcac cggcatcatc aggtcgagca
     3901 ggatcagcga cggcggcgac gcgctggtgc gcaggatctc gagcgcctcg acgccgttgc
     3961 ccgcaccgat cgacggatag ccgtgctcgt cgagcagctc gaccaggctg tcgcggatgt
     4021 cgcgatcgtc gtccaccacc aggatacagc gggccatgtc acgcgaccag atcgtgggaa
     4081 gtcgagtcga gcggcagccg caccgtgaag caggcgccgc cgccggcgga gttctcggcg
     4141 gtgacctgac cgccgtgtgc ctcggtgatc tggcgcacca cgtagagccc gaggccgagg
     4201 cccgcgtagt ggcgcatcga ggtcccccgc tcgaagcgct cgaacatccg cgcgatctgt
     4261 ccgtcgggca gccccggccc ctggtcgcgg acctcgatca ccgcgtcgcc ggcgcggcgc
     4321 tcgaccgtga ccgtgatcgg cgagccggcg gcgtagcgga tcgcgttcga taccaggttg
     4381 gtcacgatct ggtcgatccg cgagcggtcc caggtgccga tcgcgtggtc ggcggtgacc
     4441 gacagcgtgc accccgcgtt ggccgcgacc tcgcgcaggc ggtcgaccgc ggtcgcgacg
     4501 atgtcggcga ggtcggcctg ctcgaggtgg agggtgaacc ggccggtcgc gatccgcgac
     4561 acgtcgagca gcgcgtcgat cagctcggtc agccgctggc cggcgcggtc gctgcgctcg
     4621 agcttgacca gcgtcgcgcg gtcgtcgccg gtgatccgct cgcgcagcgc ctcgagctgc
     4681 agccgcagca cggtcagcgg ggtcttgagc tcgtgcgatg ccagcgacag gaactcgtcg
     4741 cgcagccgga ccgcctcctc ggcgtgggcg atccggacgc gctcctcgtc gaggaggcgg
     4801 cgctcggtga ggtcgcgggt gaccttggcg tagccgatca gcgcgcccga cggatcgcgc
     4861 agcgcggtga tcacgacgtt ggcccagagc cgggtgccgt ccttgcgcag ccgccagccc
     4921 tcgtcctcga accggccgtc gcgcgccgcg gcccgcagct cgtactcgca cttgccggcc
     4981 cggacctcgt catcggaata gaacaccgag aagtggcggc cgatgatctc gtgcgcggcg
     5041 tagccgttga tccgctgcgc gccgacgttc caggtcgcga ccttgccgtc ggcgtcgagc
     5101 atgaagatcg cgtattcctt gatggcctcg accaggacgc ggaaccgctc ctcgtgctgg
     5161 cgggtggcct cccgcgcgtc gcccgccgcg gcgtcccgcg tggacccggt ggtcgcctcg
     5221 ggggtcgcgt cgatggacgc ctgggtgggg gcgggcgcgg cggcgtcgcg cacgatcggg
     5281 atcgggcccg acttgcccgc cggcagctcg gcgtcgaacg tcgcgatccg cgcgccggtc
     5341 gtgcgccgcg gcaggtcgaa ccggaacgcg gtccagccgt ccaccgactc ggccgcgatc
     5401 gagccgccgt gggcctgcac gatctgcgcg gtgatgaaca gcccgagccc gaggccctgg
     5461 ctgcgcagcg ccttctgctg cgatccgcgg aacggcgaga acaggatcgg cagcacctcg
     5521 ggcgggatcg tgccgcggtt cttgacctcg acgcggatcg cgtcgcgatc gcggccgtcg
     5581 atcagcaccg tgagcggcgc gtcggtcgag ccgtgctgca ccgcgttgcc gatcaggttc
     5641 gagaacacct gcgacagccg gttgccgtcg tggtggccgc agacgtcgcc gtggaccacg
     5701 agctcgatct tccagtccgg ccgcgcgagc cggacctcct cgatcacgtc gcgcgcgatg
     5761 tcggggacct cgagcggctg gcggtcgagc tcgatgccgc cgtcggcgcg cgcccgcgcg
     5821 aagtcgagca gctggctgat catgcgctgc atgcggtcgg cgctgttgag caagcgctcg
     5881 atcgtgcgca ccggcttggg ctggtcgacg tggccgagca gcagctggcc ggccatcacg
     5941 atcgtgctga gcgggccgcg caggtcgtgg ccgacgatcc cggcgaacat gtcgttgagc
     6001 cggacggtgc gggtcagctc ctcgatcagc tgggtgcgct cgcgctcggc gcgcctgtcc
     6061 gcggtgacgt cgcgcaggaa gccgcgcgac acgccgcagc cgcggccgtc gagcctcatg
     6121 ctctcgctga tcagcacgtg gcggatcgcg ccgtcgtgcg cgcgcagccg gacctcgcag
     6181 tcgcgcacgg tctcgccgcg ccgcagccgg tcgagcaggc cggcggccac gtcgggatcg
     6241 acgcagaacg ccgacagcgg gcggccgatg tagtcctcgg cgccgtaccc gagcaagtcg
     6301 agctcggcgc gattggccca cacgatcgtc cccgccgcat cgatgcggtg gatcgcgacc
     6361 ggcgcgtcgt cgaggagctc cgccaagtcg ccggtcgact cgggcagaac gcggtaggac
     6421 gggttcgcgg tgaccgggga catacgtgcg agtgcgtatc ctgttgaggg gtctggggtt
     6481 cgaaccgggc agccgcccga acgtgcaggg cagagatgca ggttttgtgc accgagagat
     6541 cggcgtaaac gcggtaaccg ggcgcgccgg gatcacgacg acggaggcgc tctgtctcgc
     6601 gtccgcacgg cgtgcgcacc cgccacgctt gggcttgcac cggccatgct acggtttgcc
     6661 ggtgggaatt acccggcgca tcctggagag cgcacaatcc ggactgtctc gcctgacctc
     6721 gctcgtgatc gtcgatgacg aaccgctgag ccatgtcgag agcgccgcgc tgcaggccga
     6781 gctcacggtg cgcaaggccg ggcgcgagcg ggcgccccgg ctcgcccagg acaacccgct
     6841 ggcgaagctc gcgagctcgg atccggcggc gcgggcggcg cgggcggcgg cc
//
LOCUS       DASVOR010000185         3479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_264720,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000185 DASVOR010000000
VERSION     DASVOR010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264720"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF516_04155"
     CDS             complement(<1..203)
                     /locus_tag="VF516_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 family protein"
                     /protein_id="HEX8106894.1"
                     /translation="MSSEAVSNAVIEHFLEQAGWCEHYGSPFTARLLERLARDIEAGG
                     PTAELVAGWPRSPRADALSLRIAG"
     gene            296..1504
                     /locus_tag="VF516_04160"
     CDS             296..1504
                     /locus_tag="VF516_04160"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8106895.1"
                     /translation="MRVVFLSPRYPPEMRQFTRGLAEVGAEVLGVGDGSPEPELRRYL
                     ADYLEVPSLLDEDDVIARVHAWLRGRSIDRVLANWEPLVVVAARLRERFGLPGMSVDT
                     VRGFRDKQLMKDRVAAAGLRVPRAQRVRSVAEVWGAVEHTGFPAIIKPISGAGSADTY
                     KVESRADMERVLPRLHHVTEAICEEFIAGDEYTYDTVSIAGQPAYESVTRYLPNALEM
                     RSQEWVSPIMLTVRDLDQPHVRGGIELGRRVLTALGMGDGMTHMEWFRTASGEVVFGE
                     VACRPGGACVVDLMNYTSDIDLFREWARVATAGRFDASTERTYNVGIIFKRARGQGRI
                     TAIAGLTEYYDRYRAHIVEDTLLRPGTPRRDWKATLLSDGYLVVRHPDWDQAQQMAFA
                     AATDIQLYAS"
     gene            complement(1492..1866)
                     /locus_tag="VF516_04165"
     CDS             complement(1492..1866)
                     /locus_tag="VF516_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106896.1"
                     /translation="MEIEIACSASVAGLLIRRAAASAPAISVSSTGMAMENSELLDRP
                     DSQPIDVPINTTATKFPIAPSTANILAFICVAPDLWTSMASTPCARRSRRPRERLPLA
                     SLPDRLPPFDGASVTSAHSYDA"
     gene            1880..2287
                     /locus_tag="VF516_04170"
     CDS             1880..2287
                     /locus_tag="VF516_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106897.1"
                     /translation="MLGLAGGAALGTMARLGDWFAPDPERIIARWDRWRAILPPDDVV
                     RRVYERTLTDPYRVNRPAPPPTTQGSEVVLDGAPLCRPVVARADRLGLEDLKTALRDN
                     HVPWAAAVTPIQDVAMLRDVAKDLCTWEASLRR"
     gene            2313..2645
                     /locus_tag="VF516_04175"
     CDS             2313..2645
                     /locus_tag="VF516_04175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8106898.1"
                     /translation="MCRPRGPAAVHFHDIARAGTVGEVVGFPVASSLVADLAPVALRG
                     RYQGVNAMVSGMSMALSPILGGQVIDQLGAPALWMLCLGIGAAVAAGHLLAAPARRRR
                     LAALTARA"
     gene            complement(2629..>3479)
                     /locus_tag="VF516_04180"
     CDS             complement(2629..>3479)
                     /locus_tag="VF516_04180"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8106899.1"
                     /translation="VRGMASEVIARELVPPEFPERELVVPQPVTFRAADGLTIHGQLF
                     APAACKAGCRAVVFSHGGPIRQMLPAWHYMDYYANAYAENQFLAGQGIVVLSVNYRLG
                     IMYGHDFREAPGGSWRGSSEYQDVVAAAGYLASLPQVDKQRIGLWGGSYGGLLTALGL
                     ARNPELFKAGVDFHGVHDWSVLLGDGEDGFADAPDKKEAQELALRSSPLADVARWRSP
                     VLLIHGDDDRNVPFDQTTDLVQHLRRQGVRVEEIVYPDEIHDLLLWRSWIDAYRATAA
                     FFTREL"
BASE COUNT          485 a         1214 c         1266 g          514 t
ORIGIN      
        1 ccggcgatcc gcagcgacag cgcgtcggcg cgcggcgatc gcggccagcc ggcgaccagc
       61 tcggccgtcg gaccgccggc ctcgatgtcg cgcgcgagcc gctcgagcag gcgcgccgtg
      121 aacggcgagc cgtaatgctc gcaccagccg gcctgctcca ggaagtgctc gatcaccgca
      181 ttggacacag cttcggacga catggccatt gatcgcccca accggcgtgc cgcgcaagca
      241 ccgtgttgca cggcccggct tgtcgcggcc ggcgccggcg gataccgtcg gcgggatgcg
      301 cgtcgtgttc ctgtccccga ggtatccgcc cgagatgcgg cagttcacgc gtggcctcgc
      361 cgaggtcggc gccgaggtgc tcggcgtcgg cgacggcagc cccgagcccg agctgcggcg
      421 ctacctcgcc gactacctcg aggtcccgtc gctgctcgac gaggacgacg tgatcgcccg
      481 ggtccacgcc tggctgcgcg gccggtcgat cgatcgcgtg ctcgccaact gggagccgct
      541 ggtcgtcgtc gcggcgcggc tgcgcgagcg gttcgggctg cccgggatgt cggtcgacac
      601 cgtgcgcggg tttcgcgaca agcagctgat gaaggaccgg gtcgccgccg ccggcctgcg
      661 ggtgccgcgc gcgcagcgcg tgcggtcggt cgccgaggtg tggggcgcgg tcgagcacac
      721 cgggttcccg gcgatcatca agccgatcag cggcgccggc agcgccgaca cctacaaggt
      781 cgaatcgcgc gccgacatgg agcgcgtgct gccgcggctc caccacgtca ccgaggcgat
      841 ctgcgaggag ttcatcgccg gcgacgagta cacctacgac acggtgtcga tcgccggcca
      901 gccggcctac gagagcgtca cccggtacct ccccaacgcg ctcgagatgc ggtcgcagga
      961 gtgggtgtcg ccgatcatgc tgacggtgcg cgacctcgac cagccgcacg tgcgcggcgg
     1021 catcgagctc ggccgccggg tgctgaccgc gctcggcatg ggcgacggga tgacccacat
     1081 ggagtggttc cggaccgcga gcggcgaggt cgtgttcggc gaggtcgcgt gccggccggg
     1141 cggcgcgtgc gtggtcgacc tgatgaacta caccagcgac atcgatctgt tccgcgagtg
     1201 ggcgcgggtc gcgaccgccg ggcggttcga cgcgtcgacc gagcgcacgt acaacgtcgg
     1261 gatcatcttc aagcgcgccc gcggccaggg ccggatcacg gcgatcgcgg ggctcaccga
     1321 gtactacgat cgatatcgcg cgcacatcgt cgaggacacc ctgctccggc cgggcacgcc
     1381 gcggcgcgac tggaaggcga cgctcttgtc cgacggctat ctcgtggtcc gccacccgga
     1441 ctgggaccag gcccagcaga tggcgttcgc cgcggcgacc gatatccagc tctacgcgtc
     1501 gtagctgtgc gccgaggtca cgctcgcccc gtcgaacggt ggcaagcgat cgggaaggct
     1561 ggcaagcggc aggcgttcgc gtggacgacg cgagcgacgc gcgcaaggcg tgctagccat
     1621 cgaagtccag agatccgggg caacacatat gaatgcgagg atgttggcgg tgctcggggc
     1681 gatcgggaac ttcgtcgccg tggtgttgat cggcacgtcg atcggctggc tgtcgggcct
     1741 gtcgagcagc tcgctgttct ccatcgccat gccggtgctg ctcacgctga tcgccggtgc
     1801 gctggccgcg gcccgcctga tcagtaaacc agccacggac gcgctgcagg cgatctcgat
     1861 ctccacgatc acgatcttcg tgctcgggct cgccgggggc gccgcgctcg gtacgatggc
     1921 gcggctgggc gactggtttg cgcccgatcc ggagcgaatc atcgcgcggt gggaccgctg
     1981 gagagcgatc cttccacccg atgacgtggt gcgccgggtg tacgagcgga cgttgacgga
     2041 tccctatcgc gtgaaccgtc ctgctccgcc gccgacgacg cagggcagcg aggtcgtgct
     2101 cgatggcgcc cccctgtgtc gcccggtcgt tgcccgtgcc gatcgcctgg gcctcgagga
     2161 tctgaagacc gccctgcggg acaaccatgt cccgtgggcc gcggccgtca caccgatcca
     2221 ggatgtggcg atgctgcgcg atgtcgcgaa ggatctgtgc acctgggaag ccagcttgcg
     2281 ccgctgaggc tgcccgtccg cctgcgtacc gggtgtgccg tcctcgagga ccggcggctg
     2341 tacatttcca tgacatcgcc cgggccggga ccgtcggtga ggtcgtcggg ttcccggtcg
     2401 catcgtcgct ggtcgccgac ctggcgccgg tcgcgctgcg cggccgctac cagggcgtga
     2461 acgcgatggt gtcgggcatg agcatggcgc tgtcgccgat cctcggcggg caggtgatcg
     2521 atcagctcgg cgcgcccgcg ctgtggatgc tgtgcctcgg catcggcgcc gcggtcgccg
     2581 ccggtcacct gctcgcggcc ccggcgcggc gccgccggct cgccgcactc acagctcgcg
     2641 cgtgaagaac gccgcggtcg cgcggtacgc gtcgatccag ctccgccaca gcagcaggtc
     2701 gtggatctcg tcgggataga cgatctcctc gacccgcacg ccctggcgcc gaaggtgctg
     2761 gaccagatcg gtggtctggt cgaacgggac gttgcgatcg tcgtcgccgt ggatgagcag
     2821 caccggcgat cgccagcgcg cgacgtcggc cagcggcgag gacctgagcg ccagctcctg
     2881 cgcctccttc ttgtcgggcg cgtcggcgaa gccgtcctcg ccatcgccga gcagcaccga
     2941 ccagtcgtgc acgccgtgga agtcgacgcc ggccttgaac agctccgggt tgcgcgcgag
     3001 gccgagcgcg gtgagcaggc cgccgtacga gccgccccac aggccgatcc gctgcttgtc
     3061 gacctgcggc agcgacgcca ggtagccggc ggcggcgacg acgtcctggt actcggacga
     3121 gccgcgccag ctgccgccgg gcgcctcgcg gaagtcgtgg ccgtacatga tgccgagccg
     3181 gtagttcacc gacaagacga cgatgccctg gccggcgagg aactggttct cggcgtaggc
     3241 gttggcgtag tagtccatgt agtgccacgc gggcagcatc tggcggatcg ggccgccgtg
     3301 actgaacacg accgcgcggc agcccgcctt gcacgccgcc ggcgcgaaca gctggccgtg
     3361 gatcgtgagc ccgtcggcgg cgcggaacgt caccggctgc ggcacgacga gctcgcgctc
     3421 ggggaactcg ggcggcacca gctcgcgcgc gatcacctcg gacgccatgc cgcgcaccc
//
LOCUS       DASVOR010000186         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_177247,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000186 DASVOR010000000
VERSION     DASVOR010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177247"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(247..1239)
                     /locus_tag="VF516_04185"
     CDS             complement(247..1239)
                     /locus_tag="VF516_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8106900.1"
                     /translation="MKVSNLCLGAMTFGEADEKSFMHKAGSDEATAHAVLDRAVAAGI
                     NFVDTADVYGQDGLSERVLGNWLVARGSRDKIVLATKFRFTMGDGPNRSGASRYRIMK
                     CVDDSLRRLRTDRIDLYQIHMQDITVPEDETVRALDDLVRAGKVLYIGCSNYAAYRLM
                     RSLWLSRTHHRAAFVTLQAQYSLLVRELEREHVPLCRDEGLGILPWSPLAGGFLTGKF
                     ERGKPPQAGTRLGAKDRLARYDLERNWKILDAVRGVAAETGSTPSAVSIAWLLAKPQV
                     TSVIFGARTVEQLEANLAAAELALTPAQLAALDQASAFDLGYPYSFIQATQSSW"
     gene            1357..>2572
                     /locus_tag="VF516_04190"
     CDS             1357..>2572
                     /locus_tag="VF516_04190"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106901.1"
                     /translation="MTSAVWEEGTWRAVRSPVAVTHRVGPDRPCDPRTLAAAPAAAGD
                     LAEQALQDAARDTTEQPAVEVVGAPIDDAEEATEADALPADEETTTLRPPRISRDLML
                     WEAATVSAATRDPARSRGRIGKYELSARLARGAFGVVYTARDPSLERNVAIKVLRLSH
                     LANQEIVQRFLQEARATARIAHPGIVTIHDFGMVETRRGSTAFIAMELLSGESLSRRL
                     ARERRLAPAAACEIVRQVASALEAAHHVDVLHRDLKPDNIFLVPDPAMPSGERVKVLD
                     FGLAKLGASGHTQTQMVFGTPRYISPEQSRSATQIDHRSDIYSLGCILFELVTGRTPF
                     DGDPRQLIERHQRMTPPRAAALAPEVPPVLDELIADMLAKDPRDRPQTMAAVQRVLRF
                     AANGDAEAPPAAL"
BASE COUNT          399 a          914 c          901 g          358 t
ORIGIN      
        1 accgccgacg ccgcaagccg actgccgact gccgactgcc gactgccgac gccgtcagcc
       61 gacgccgcca gccgacgcca cgctccgacg accgacagga gcttctgcca gatcgccaat
      121 aaattcggtc atctgagccg atcggcaacg attccaaaac ggaaatagtt ggctccgccg
      181 aagatcagta gtttccattt tggaaactac tgatcttcga cgaccccgcg gtcgccgctg
      241 ggccggtcac cagctcgact gggtcgcctg gatgaacgag tacgggtagc cgaggtcgaa
      301 cgccgaggcc tgatcgagcg cggcgagctg ggccggggtc agggcgagct cggcggcggc
      361 gaggttggcc tcgagctgct cgacggtccg cgcgccgaag atcaccgagg tgacctgcgg
      421 cttggcgagc agccacgcga tgctgaccgc cgacggggtc gacccggtct cggcggccac
      481 gccgcgcacg gcgtcgagga tcttccagtt gcgctcgagg tcgtagcgcg ccaagcggtc
      541 cttcgcgccc agccgggtcc cggcctgcgg cggcttgccg cgctcgaact tgccggtgag
      601 gaagccgccg gcgagcggcg accacggcag gatgccgagg ccctcgtcgc gacacagcgg
      661 gacgtgctcg cgctcgagct cgcggaccag caggctgtac tgggcctgga gcgtgacgaa
      721 tgccgcccgg tgatgggtgc gcgacagcca caggctgcgc atcagccggt acgcggcgta
      781 gttcgagcag ccgatgtaga gcaccttgcc ggcgcgcacc aggtcgtcga gcgcgcgcac
      841 ggtctcgtcc tcgggcaccg tgatgtcctg catgtggatc tggtagaggt cgatccgatc
      901 ggtgcgcaat ctgcgcaatg aatcgtccac gcacttcatg atccggtaac gcgacgcgcc
      961 gctgcggttg gggccatcgc ccatcgtgaa ccggaacttg gtcgcaagga cgatcttgtc
     1021 gcgcgacccg cgcgccacga gccagttgcc gagcacgcgc tcggacagcc catcctggcc
     1081 gtagacgtcg gcggtgtcga cgaagttgat gccggcggcg accgcgcggt cgagcacggc
     1141 gtgcgcggtc gcctcgtccg agccggcctt gtgcatgaac gacttctcgt cggcctcgcc
     1201 gaacgtcatc gcgcccaggc agagattcga caccttcaca cccgatgatc cgagattgcg
     1261 atatttcatc tgcgttacct cgggccgacc gtaacaccgc cggtgcaccg cggtggcaac
     1321 tggggcctgg gctcctcacc acggacgcgc aacctcatga cgagtgccgt gtgggaggaa
     1381 ggcacgtggc gtgcagtgcg cagccccgtg gcagtcaccc accgcgtggg cccggatcgg
     1441 ccgtgtgatc cccgcacgct ggccgcggcg cccgccgccg ccggagacct ggccgagcag
     1501 gcgctgcagg atgcggcacg cgacaccacc gagcagccgg cggtggaggt cgtcggggcg
     1561 ccgatcgacg acgccgagga ggcgaccgag gccgacgcct tgccggcgga cgaggagacg
     1621 accacgttgc gcccaccgcg gatctcgcga gacctgatgc tgtgggaggc cgcgaccgtg
     1681 agcgccgcca cccgcgatcc ggcgcgctcg cgcggccgca tcggcaagta cgagctctcc
     1741 gcccggctgg cccgcggcgc gttcggcgtg gtgtacaccg cgcgcgatcc gagcctggag
     1801 cgcaacgtgg cgatcaaggt gctgcgtctg tcacacctgg ccaaccagga gatcgtccag
     1861 cggtttctcc aggaggcgcg cgccaccgcc cggatcgccc accccgggat cgtgacgatc
     1921 cacgacttcg ggatggtgga gacccggcgc ggctcgacgg cgttcatcgc gatggagctc
     1981 ctgtccggcg agagcctgtc ccgccggctg gcgcgcgagc gccggctggc cccggccgcc
     2041 gcgtgcgaga tcgtgcgcca ggtcgcgtcc gcgctggagg cggcgcacca cgtcgacgtg
     2101 ctgcaccgcg acctcaagcc cgacaacatc ttcctggtcc ccgatccggc gatgccgagc
     2161 ggcgagcggg tgaaggtgct cgacttcggg ctcgccaagc tcggcgcgag cggccacacc
     2221 cagacccaga tggtgttcgg cacgccgcgc tacatctctc cggagcagag ccgctcggcg
     2281 acccagatcg accaccgcag cgacatctac tcgctcggct gtatcctgtt cgagctggtg
     2341 accgggcgca caccgttcga cggcgacccg cgccagctga tcgagcgcca ccagcgcatg
     2401 acgccgccgc gcgccgccgc gctggcgccc gaggtgccgc cggtgctgga cgagctgatc
     2461 gccgacatgc tcgccaagga cccgagggac cggccgcaga ccatggctgc ggtgcagcgc
     2521 gtgctgcggt tcgccgcgaa cggcgacgcc gaggcgccgc cggcggcgct gg
//
LOCUS       DASVOR010000187         1588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_30985,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000187 DASVOR010000000
VERSION     DASVOR010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30985"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VF516_04195"
     CDS             <1..506
                     /locus_tag="VF516_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106902.1"
                     /translation="VFDVPINAVADYSNAERVNRVARSLAPDVETRLISIADDSFGAM
                     LAIRAAIDRGELVAMLGDRPGATSDAARVVMAPFLGADAAFPAGPWLLAHTLGCPVYF
                     VAGVFTSPNHYALHFELLADEVRLDRKDRAGSLAGYVRTYAAMLETHVRSAPMNWFNF
                     YDFWSPP"
     gene            503..>1588
                     /locus_tag="VF516_04200"
     CDS             503..>1588
                     /locus_tag="VF516_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HEX8106903.1"
                     /translation="MTPLCIGEARVTHGDVARVARGELTVALTEAASWHDRMRASRRA
                     VDARAQSGEPFYGVNTGFGASVVNTVAAEHGGNLAVNLFRYHGCGVGPPLAPHEVRAV
                     IVARLAQLAAGWSGIRVETLRAMVALLEHDLLPVIPELGSVGASGDLTPMSYVAAVLS
                     GEREVSVAGRTVPAAEALAAAGLPPITLGPKETLSIMNGTSVMTALVSLALDRAERFT
                     RAHARVTAWNSLLLRGQPAHFDERLFLAKPHPGSVRYAGWVRADLAAGPRPELRPGKV
                     QDPYSVRCAPHVVGVLLEVLDHAAATVAVELNGAGDNPLICEHTHAVLHGGNFYGSHI
                     THVADTVKLHLAHAAEVLERQLVLLCHS"
BASE COUNT          192 a          638 c          530 g          228 t
ORIGIN      
        1 gggtgttcga cgtgccgatc aacgccgtcg ccgactactc caacgccgag cgcgtcaacc
       61 gggtcgcccg ctcgctggcg cccgacgtcg agacccggct gatctcgatc gccgacgact
      121 cgttcggcgc gatgctcgcg atccgcgccg cgatcgaccg cggcgagctc gtcgcgatgc
      181 tcggcgaccg gccgggcgcg acgtccgacg cggcgcgcgt cgtgatggcc ccgttcctcg
      241 gcgccgacgc cgcgttcccg gccgggccgt ggctgctcgc ccacacgctc ggctgcccgg
      301 tgtacttcgt cgccggcgtg ttcacgtccc ccaaccacta cgcgttgcat ttcgagctgt
      361 tggccgacga ggtgaggctc gaccgcaagg accgcgccgg ctcgctcgca ggctatgttc
      421 ggacctacgc cgcgatgctg gagacccacg tgcgctcggc gccgatgaac tggttcaact
      481 tctacgactt ctggagcccg ccgtgacccc gctgtgcatc ggcgaggccc gcgtcaccca
      541 cggcgacgtc gcgcgggtcg cccgcggcga gctcaccgtc gcgctgaccg aggccgcgag
      601 ctggcacgac cggatgcgcg ccagccgccg ggcggtcgac gcgcgcgcgc agagcggcga
      661 gccgttctac ggcgtcaaca ccgggttcgg cgcctcggtc gttaacaccg tcgccgccga
      721 gcacggcggc aacctcgccg tcaacctgtt ccggtaccac ggctgcggcg tcggcccgcc
      781 gcttgcgccc cacgaggtcc gcgccgtgat cgtcgcgcgg ctcgcccagc tcgcggcggg
      841 ctggagcggc atccgggtcg agaccctgcg cgcgatggtc gccctgctcg agcacgacct
      901 cctgcccgtg atccccgagc tcggctcggt cggcgcgagc ggcgatctca cgccgatgtc
      961 gtacgtcgcc gcggtgctgt cgggcgagcg cgaggtctcg gtcgccggcc gcaccgtgcc
     1021 cgccgcggag gcgctcgccg ccgccggcct tccgccgatc accctcgggc ccaaggagac
     1081 cctgtcgatc atgaacggca cgagcgtgat gaccgcgctc gtcagcctgg cgctcgatcg
     1141 cgccgagcgg ttcacccgcg cccacgcgcg ggtcaccgcg tggaactcgc tgctcttgcg
     1201 cggccagccg gcgcacttcg acgagcgcct gttcctcgcc aagccgcacc caggctcggt
     1261 gcgctacgcc ggctgggtgc gcgccgatct cgccgcgggc ccgcggcccg agctccgccc
     1321 cggcaaggtc caggatccgt actcggtgcg ctgcgcgccg cacgtcgtcg gtgtgctgct
     1381 cgaggtcctc gaccacgccg ccgccaccgt cgcggtcgag ctcaacggcg ccggcgacaa
     1441 cccgctgatc tgcgagcaca cccacgccgt gctgcacggc ggcaacttct acggcagcca
     1501 catcacccac gtcgccgaca ccgtcaagct ccacctcgcc cacgccgccg aggtcctcga
     1561 gcgccagctc gtcctgctct gccactcg
//
LOCUS       DASVOR010000188         6229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_60301,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000188 DASVOR010000000
VERSION     DASVOR010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60301"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /locus_tag="VF516_04205"
     CDS             complement(<1..388)
                     /locus_tag="VF516_04205"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8106904.1"
                     /translation="MSRFLPSNGIRMHLIEEGSGPLVVLLHGFPELSTTWRHQLRALA
                     DAGYHAVAPDQRGYGQTDCPEREDAYTMLHLVGDVIGLLDALDEREAVVVGHDWGAPV
                     AWNAALLRPDRVRAVVGMSVPPRDRPP"
     gene            complement(485..775)
                     /locus_tag="VF516_04210"
     CDS             complement(485..775)
                     /locus_tag="VF516_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106905.1"
                     /translation="MRAVAQAYLDSDAFPDQVFDHSAAQSVIDQHFEHGQIRLEEVGM
                     LLTLATASHLLSGDLRTVPAEAQPDLSTTRTTGRRKSVRTGARFESSSHASP"
     gene            complement(946..2247)
                     /gene="nhaA"
                     /locus_tag="VF516_04215"
     CDS             complement(946..2247)
                     /gene="nhaA"
                     /locus_tag="VF516_04215"
                     /inference="COORDINATES: protein motif:HMM:NF018648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HEX8106906.1"
                     /translation="MRSLERFIHVQAMSGVVLLAAAAIALAWANSPWGGVYEAVLATR
                     FELQVGRWSVGTSLKFLVDDVLMALFFFVIGLELRRRMHGGELGTLREAALPIVAALG
                     GMVVPAAIYVMAVGLFGDASVGWGVPVSTDTAFALGVLALLGSRIAPNVRVLLLAIAI
                     IDDVVAVLVIAIFYSSRIDPRGICVAVAGISAVLILQRFGVRRVASYVIPAMAVWIGI
                     WWAGIHPTFAGIIVGLLTPARTWYGHEGLADAARQHIDVIDRPASNGGTRLRDLARSR
                     LELRRAHREAVSPVERIEAALHTWTAFAIMPVFVFANAGIAFGQVDVAAAPRLAAGVV
                     AGLVVGKLAGIVLASAGAVKLGIARLPIGVTWRGVAVVGAVAGIGFTMALFIANLAFA
                     ERPDLRDVARAAILCSSAVSVVLALLVGRAVPSKCGTDTRR"
     gene            complement(2360..2605)
                     /locus_tag="VF516_04220"
     CDS             complement(2360..2605)
                     /locus_tag="VF516_04220"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8106907.1"
                     /translation="MLVADDDRDLLEMVSRVLGRLGLEVGIASSGAELLEKLGEQGPF
                     DLVVTDVSMPWISGLQVMHSARTAGMRCPIVVIASSD"
     gene            3000..3260
                     /locus_tag="VF516_04225"
     CDS             3000..3260
                     /locus_tag="VF516_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEX8106908.1"
                     /translation="MALALDSVRVPRHVDVFGEAAMAEGTIKRLTDKGFGFIDNGSGK
                     DLFFHMSAVEGCRFEDLREGQKVTYNVGRGAKGPRAENVRVI"
     gene            3373..3693
                     /locus_tag="VF516_04230"
     CDS             3373..3693
                     /locus_tag="VF516_04230"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX8106909.1"
                     /translation="MSNRLYVGNLPFHATEDLISQRVAQCGEVRGVDLIIDRVTGRPR
                     GFAFVEMATAEGMRKAIAELDGVNFEGRPLTVKVAEDRRDGSGGPRGSGGPRGGDRGW
                     GRGR"
     gene            3683..3958
                     /locus_tag="VF516_04235"
     CDS             3683..3958
                     /locus_tag="VF516_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106910.1"
                     /translation="MAANVERAPGALANRASQIGEAWARDYVRELREQKRAPVGAWPG
                     TMREARMRVRSRLAIVLDPDRLEELAHVANLAARQGWRKLCEPDLEP"
     gene            complement(4207..4875)
                     /locus_tag="VF516_04240"
     CDS             complement(4207..4875)
                     /locus_tag="VF516_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8106911.1"
                     /translation="MGKTTGMAGGDPTGTDRRQHVRVVANGSAIVYGHATTRGRLSNI
                     SSGGMVMRPGEGEYRCGSGEYVEVELHLDRAGATWLRFHGEVVRADHSELAISFTAVP
                     LDFAHVVQDALVSALEGAGLAHVLLVDANTQRRGPFAALLRRAGCRVAEVATPLDAIA
                     HLGGSAIHSWIVAVASTVPASTAEELQRFLSESDPPIDVRMLGNKSPTSALAWFTATA
                     RRPD"
     gene            complement(5003..5794)
                     /locus_tag="VF516_04245"
     CDS             complement(5003..5794)
                     /locus_tag="VF516_04245"
                     /inference="COORDINATES: protein motif:HMM:NF004795.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8106912.1"
                     /translation="MISYVNVRHETHDRGRIAYVTVDHAAKLNSLSSEVMDQFIDRFR
                     GLAADLALRAVVLTGAGDKAFIGGANIDEMAALDGPQAARAFILKVHGCCHAVRQTPA
                     PVIAAVNGWCLGAGLEVAAACDLRLAADTAQFGMPEVKLGLPSVVEAALLPSLIGWGR
                     TRRILMTGETFGAAEALAWGLIEEAHPPALLFAAVEDLLDKLLAAEPRAVQLQKALIQ
                     AWEEAPISRAIAAGVDAFEDAWTTDEPAHAMARFLAERRAVKGDT"
     gene            complement(5944..>6229)
                     /locus_tag="VF516_04250"
     CDS             complement(5944..>6229)
                     /locus_tag="VF516_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106913.1"
                     /translation="ARRTPDMPLYTLRNKTTGEIVKVTDPGRALIDGKWSHIGRFKGP
                     MLRNLAPRAPYFHNGFAKDLWAVVNFYDERFHIGFTKQEKSDLVAFLRSL"
BASE COUNT         1054 a         2116 c         2133 g          926 t
ORIGIN      
        1 gcggcggccg gtcgcgcggc ggcacgctca tgccgaccac ggcgcggacc cgatccggcc
       61 gcagcagcgc ggcgttccac gccaccggcg ccccccagtc gtgcccgacg acgaccgcct
      121 cgcgctcgtc gagcgcgtcg agcaggccga tcacgtcacc gaccaggtgg agcatcgtgt
      181 acgcgtcctc gcgctcgggg cagtcggtct gcccgtagcc gcgctggtcg ggcgcgaccg
      241 cgtggtagcc ggcgtcggcg agcgcgcgca gctggtggcg ccacgtggtc gacagctcgg
      301 gaaagccatg gagcagcacg acgagcgggc cgctgccctc ctcgatcagg tgcatccgga
      361 tgccattgga cgggaggaag cgcgacattg tccgaagcgt actgcggcgc cgcccgcggg
      421 cacgtccgcg cacgccggtt tggaatcaga tcaccgcgac gccgtgcagg gaagggatca
      481 cacctcacgg tgatgcgtgg ctgctggatt caaaacgcgc cccggtgcgc acggacttgc
      541 ggcggccagt cgtgcgggtg gtcgacaggt ccggctgcgc ctcggccggg acggtgcgaa
      601 ggtcgccgga cagcaagtga gatgcggtgg ccagcgtgag cagcatgccg acttcctcga
      661 gccggatctg gccgtgctcg aagtgttgat cgatcaccga ctgcgcggcg ctgtgatcga
      721 acacctggtc gggaaatgcg tccgagtcga ggtacgcctg cgcaacggcc cgcaacgcgc
      781 tggatcgact gtccgcggga accattgcac cggctcgccc gtgccgagcc gaaacatgcg
      841 gcgatgaact cgatcgagag gcactcgggg aacgccagcg tgccctcggt gcgccatacc
      901 acccgcggca ggccgtaccg ggcagcgcag gcgacggacc cgccgtcacc ggcgagtgtc
      961 ggttccacac ttgctcggca cggcgcgccc gacgagcaac gcgagcacga ccgatacggc
     1021 ggacgagcac aggatggccg ccctcgccac gtcgcgcaga tccgggcgct cggcgaaagc
     1081 caggttggcg atgaacaagg ccatcgtgaa cccgatgccg gcgaccgcac cgacaacggc
     1141 gaccccgcgc cacgtcacgc cgatcggcag ccgcgcgatg ccgagcttca ccgcacctgc
     1201 gctcgcgagc acgatccccg cgagcttgcc cacgacgagg ccggccacca cgcccgcggc
     1261 aaggcgcggc gccgccgcca catcgacctg tccgaaagcg ataccggcgt tcgcgaacac
     1321 gaacaccggc atgatggcga atgcggtcca cgtgtgcagg gcggcctcga tccgttcgac
     1381 cggcgacacg gcctcgcgat gggcgcggcg cagctcgagc ctcgatcggg cgagatcgcg
     1441 aagcctcgtg cctccgttgc tggccgggcg gtcgatcaca tcgatgtgct gacgagctgc
     1501 gtcggcgagc ccttcgtgcc cgtaccacgt gcgtgccggc gtcaacagcc cgacgatgat
     1561 gccggcgaac gtcggatgga tgccggccca ccagatcccg atccagaccg ccatggcggg
     1621 aatgacgtag ctcgccacgc ggcgaacgcc gaaccgttgc aggatcaaga ccgcggagat
     1681 cccggccact gcgacacaga tgccgcgcgg gtcaatcctc gagctataga aaatcgcgat
     1741 cacgaggacc gccaccacat cgtcgatgat cgcgatcgcg agcaacagca cgcgcacgtt
     1801 cggcgcgatg cgcgaaccca gcagcgcgag cacgccgagc gcgaaggccg tgtccgtgga
     1861 gaccggcacg ccccagccca cgctcgcgtc gccgaacagg ccgaccgcca tcacatagat
     1921 cgccgcggga acgaccatgc cgccgagggc ggcgacgatc ggcagcgccg cctcgcgcaa
     1981 cgtgccgagc tcgccgccgt gcatccttcg ccggagctcc agcccgatga cgaagaagaa
     2041 gagcgccatc aggacgtcgt cgaccaggaa cttcagcgat gtgcccaccg accaccggcc
     2101 gacctgcagc tcgaaccgcg tcgccaggac tgcctcgtag acgccgcccc agggtgagtt
     2161 cgcccacgcc agcgcgatgg ccgccgcggc caggaggacc accccgctca tggcctgcac
     2221 gtggatgaag cgctcgagcg accgcatgac ggctcgcgac gcgcgttgcg cgccgggaga
     2281 ggcacccggg ggatgttccg aaacgacgtg atcgcgggac gacatgtggg acttcacgtc
     2341 gaaacattgc cggcgacctc taatcggacg acgcgatcac gacgatgggg caccgcattc
     2401 cagcggtgcg tgccgagtgc atgacctgga gtccggatat ccatggcatc gagacgtccg
     2461 tcacgacgag atcgaacgga ccttgctctc cgagcttctc cagtaactcg gcgccggagg
     2521 acgcgatgcc tacctcgaga ccgagccggc cgagcacgcg cgagaccatc tcgagcagat
     2581 cgcgatcgtc atcggcaacc agcacgcggc ttcgactcac gccatccgac ctgcgcatcg
     2641 agcagtagat cggtgcgagc ggacttgtac atcgcgcgtc ctcgtacacg ccctttcctc
     2701 cgagagcgtc tcaggaggtc gtctctcggc atggagttgg ccggccggtc atcgagcatt
     2761 cgcatttgct ttgcatttcg agtgccgcca acgcgcccgt ggcctgcgta ccgctcgaag
     2821 cctcgtgatc ggcgtcccag gctgcttcca aagcaccggg atcaaggggc caggcatcgc
     2881 gaaccatgcg ccgcggatcg gtgcggcggc ggcgctcatg gtcgctcggc gacctggtca
     2941 tccgctgagc acggcaaggc ccagccgcct cccgaattga ccgcgaagtc ctcgtttttg
     3001 tggcgttggc gctcgacagc gtgcgcgttc cgaggcatgt agatgttttt ggggaggcag
     3061 cgatggcgga aggtacgatc aagcgtctga ccgacaaggg cttcggattc atcgacaacg
     3121 gcagcggaaa ggatctgttc tttcacatgt cggctgtcga gggctgtcgg ttcgaggatc
     3181 tgcgcgaagg ccagaaggtg acctacaacg tgggtcgcgg cgcgaaagga ccgcgcgccg
     3241 agaacgtgcg cgtcatctag cgctagcgct tgtttcagcg aagacatccg gccgcggtgt
     3301 gcggccgttg cagattcccc gctcgcggct cgtggcatct gaccagttcc agccgccgag
     3361 agcgagtgac cgatgtcgaa ccgtctgtac gtgggcaacc ttccgttcca tgccaccgag
     3421 gatctgatca gccagcgcgt ggcgcaatgc ggggaggtgc gcggcgtcga cctcatcatc
     3481 gatcgcgtca ccggccgccc gcgcggcttc gcgttcgtcg agatggcgac cgccgagggc
     3541 atgcggaaag cgatcgccga gctcgacggc gtgaacttcg aggggcggcc gctgaccgtc
     3601 aaggtcgccg aggaccggcg ggacggcagc ggtggcccac gcggcagcgg tggcccacgc
     3661 ggtggcgatc gtggctgggg ccgtggccgc taacgtcgag cgtgcgccgg gggcgctcgc
     3721 gaatcgcgcg tcgcagatcg gcgaggcgtg ggcgcgcgac tacgtgcgcg agctgcgcga
     3781 gcagaagcgc gcgccggtcg gtgcgtggcc gggcacgatg cgcgaggcgc gcatgcgcgt
     3841 gaggtcgcgg cttgccatcg tgctcgatcc cgatcggctc gaggaactcg cgcacgtcgc
     3901 gaacctcgcc gcgcgccagg gctggcgcaa gctgtgcgag cccgatctcg agccctagcg
     3961 aggaagatcc gtcggtacgg gcgacgtcgc gggcgccggc atgtcaatgc tcctatcctc
     4021 gctggcggcc gacgcgcccc tctctagcgg cgctctgaaa gcttccatca ccatctgggc
     4081 ctatgcaacg cgcagccctt cggcaggcgc ggcactggcg agctcactct cgatcaccga
     4141 gaccagagtg cgcagggttg caacggtctt gtctccatga gccgatcgac atcgaagtct
     4201 ccgcgttcag tcgggtcttc gcgcggtggc cgtgaaccac gccagcgcac tagtcggcga
     4261 cttgttgccg agcattctga cgtcgatagg tggatcgctc tccgatagaa aacgctggag
     4321 ttcctctgcg gtgcttgccg gaaccgtgct ggcaaccgcg acgatccagc tatgaatcgc
     4381 tgaaccgcca aggtgcgcga tggcatcgag cggcgtggcc acttcggcca cgcgacagcc
     4441 ggctctgcgc agcagcgcgg cgaacggacc acgacgctgt gtgttcgcgt ccaccagcag
     4501 gacgtgcgca aggcccgcgc cttccagcgc cgagaccagg gcgtcttgca cgacgtgagc
     4561 gaaatcgagc ggtacggccg tgaagctgat cgcgagttcg ctgtggtcgg cgcgcacaac
     4621 ctcaccatgg aatcgcagcc acgtggcgcc cgcacgatcg aggtgcaact cgacttcgac
     4681 atattccccc gacccgcagc ggtactcacc ctcgccaggt cgcatgacca tgccgccgct
     4741 cgagatgttc gagaggcgac cccgcgtggt cgcatgacca tacacgatgg cgctgccgtt
     4801 ggcgacaacc cgtacgtgct ggcggcgatc ggtcccggtg gggtcaccac ctgccatgcc
     4861 tgtggttttc cccacgcgac aacatggccg caacgtaccc attcggcaag gccaatcgtg
     4921 tcacggtcac gtgcgttcgc gcccagccgg tacgtcggcg agcccgatcg gcacaagggc
     4981 tggggcgagg ccgcgcgacc gctcaggtgt ctcccttgac cgcgcggcgc tcggcgagga
     5041 accgcgccat ggcatgggcg ggctcatcgg tggtccatgc gtcttcgaaa gcgtccacgc
     5101 ccgcggcgat cgcccggctg atcggcgcct cttcccaggc ctggatcagc gccttctgca
     5161 gttgcacggc gcgcggctca gcagccagta gcttgtccag caggtcctcc accgcggcga
     5221 acagcagggc cggcggatgc gcttcctcga tcaggcccca ggccagcgcc tccgcggcgc
     5281 cgaaggtctc tccggtcatc aatatccgcc gcgtgcgccc ccagccgatc agggacggaa
     5341 gcaaggcggc ctcgacgacc gagggcagac ccagcttgac ttccggcatg ccgaactgcg
     5401 ccgtgtcggc cgccaggcgc aggtcgcagg ccgcggcgac ttccaggccg gcgccgagac
     5461 accagccgtt cacggcggcg atcaccggcg ccggggtctg gcggaccgcg tggcaacagc
     5521 cgtgcacctt gaggatgaag gcccgcgccg cctgcgggcc gtccagggcg gccatctcgt
     5581 cgatgttcgc gccgccgatg aaggctttgt cgcccgcgcc ggtgaggacc acggcgcgca
     5641 gagccaggtc cgcggccagg cctcggaacc ggtcgatgaa ctggtccatc acctcggacg
     5701 agaggctgtt cagcttggcc gcatggtcga cggtgacgta ggcgatgcgg ccccgatcat
     5761 gggtctcgtg gcggacgttc acgtaagaga tcacggttca gttatcggtt tggagctctc
     5821 ctgtcgtcaa tcagggagac atgcgcatcg gcggagcata acaatgtgag tttcgctgtc
     5881 cgaaaaggcc gagagccggc agggggaagg ccggctctcg gtggggggtg gacggaaact
     5941 tgtctagagc gagcggagga aggcgaccag gtccgacttc tcctgcttgg tgaacccgat
     6001 gtggaaccgc tcgtcgtaga agttgacgac ggcccacagg tccttggcga agccgttgtg
     6061 gaagtacggg gcgcgcgggg cgaggttgcg cagcatcggg cccttgaacc ggccgatgtg
     6121 gctccacttg ccgtcgatca gcgcgcggcc cgggtcggtc accttgacga tctcgcccgt
     6181 ggtcttgttg cgcagcgtgt acagcggcat gtccggcgtc cggcgcgcc
//
LOCUS       DASVOR010000189         4268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_352628,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000189 DASVOR010000000
VERSION     DASVOR010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352628"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1642)
                     /locus_tag="VF516_04255"
     CDS             complement(<1..1642)
                     /locus_tag="VF516_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106914.1"
                     /translation="MPGDPEHETDPGWEPPAELDGFRVVRQLGRGGMGTVYLGHDDTL
                     DRPVALKFLSSADPNAPARDRFLIEARAIARLQHPNVVGIYRVGEVLGRPYLAYELIA
                     GHSLDELAKPVAWPRALELVLGIARGLAAAHQRDVLHRDIKPANVMLSDAGEVKLLDF
                     GLAKLLDAGLRPARVPAHSGVSVIPDAIAATLGGDISLSERVTMAHGSGPPGAAADGS
                     LTAVGSILGTPLYMAPELWRGEPATMQSDVYALGLVLYELLSGRLPHHGLRGAELAEA
                     VQTRDPPPIRALCPTLPQALADVADRAIRRRREERFQSAQEVRDELEAIRALYQPFGA
                     PEATLAGDDDALLITRSFTRIAPRGDAFCARVYERLFAEHPELRSLFRIDMTAQRRKL
                     LGALMAVVDNLQAPGRLIPLLEDLGRRHAAYGVTPDSFDAVGAALLGAIADFDDQLDD
                     RCAAAWRRAYAQIAQAMQRGLATEQVTVPPGSAPPAVIAEPDPTPTRYARSGDVGLAY
                     QMVGSGAIDLVVVAGWLSHLEAAWQWPPLAGFLRRLAGL"
     gene            2083..2310
                     /locus_tag="VF516_04260"
     CDS             2083..2310
                     /locus_tag="VF516_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106915.1"
                     /translation="MVTRNLFDSATDPNECPPEFPNCTEPRVSSPASALVEPLLDTNT
                     ETAPNECPPEFPNCTEPHVGSPDPVLVERAA"
     gene            complement(2311..3561)
                     /locus_tag="VF516_04265"
     CDS             complement(2311..3561)
                     /locus_tag="VF516_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106916.1"
                     /translation="MQLAEIDFRQKRSPAESLDRAQDAIDSGALRADPEDASAYTTKS
                     YVLLRRFRIPSLTGQGDQRPLLEQIAQAAARAVELDPQDAHTWIALGNAHIYRGPYET
                     GHGGNGEVWLNQALDEFAKALAIEPSNVRAHNDLGIAHRWLATAFDKAGKDPLPEYKE
                     ALHSYERAADIDPRYLPACTNQVDLYVAIADHDNAIGVDPRTAVDSAQRVGARCLAID
                     RNFYKLHDTLAQAELALARYLVETENNPMTALTAAREALDRAEAGQHDLSLLSFHRLV
                     AANTEARFLLRSGTNPTSTIAAGRAALTKTTGLMPLTARLLVEAAHLDLAEARWAAHE
                     RQPPMPALEKARDEAEQAVTRDPGLASAYCTAAEVYLELATAQPSRASRERGIQLIDK
                     ALQLNSRLSEAKAIRAALQQLPIP"
     gene            3655..>4268
                     /locus_tag="VF516_04270"
     CDS             3655..>4268
                     /locus_tag="VF516_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106917.1"
                     /translation="MCVVVVSAVECPDRCAVDSVGGELERLGRSCRLIRDPARGTLGG
                     REVAAVQRDARLQQRGLVARGATLSKLVQRRREILPRKILLSLGEPRFVVALAAQVLE
                     GATIELAERAAARRMRLRRLLPAFGGVGQLIRRLAPLVEREVAAAERRVDERIAALAE
                     VVMGRGDRAHPVADARAIAPHIVQWMQVRILQRGLDLAGLAAEHL"
BASE COUNT          584 a         1482 c         1574 g          628 t
ORIGIN      
        1 cgaggccggc gagccggcgc aggaagccgg ccagcggcgg ccactgccag gcggcctcga
       61 ggtggctgag ccagccggcc accaccacga ggtcgatcgc gccgctgccg accatctggt
      121 aggccaggcc gacgtcgccg ctgcgcgcat agcgcgtcgg cgtgggatcg ggctccgcga
      181 tcaccgcggg cggcgcgctg ccgggcggta ccgtgacctg ctcggtggcg agcccgcgct
      241 gcatcgcctg ggcgatctgg gcgtacgcgc ggcgccaggc cgcggcgcag cggtcgtcga
      301 gctgatcgtc gaagtcggcg atcgcgccga gcagcgcggc gcccaccgcg tcgaagctgt
      361 ccggcgtcac cccgtaggcg gcgtggcgcc ggcccagatc ctcgagcagc gggatcagcc
      421 gcccgggggc ctgcaggttg tcgacgacgg ccatcagggc gcccagcagc ttgcggcgct
      481 gcgccgtcat gtcgatgcgg aacagcgagc ggagctcggg gtgctcggcg aacaggcgct
      541 cgtagacccg cgcgcagaac gcgtcgccgc gcggcgcgat ccgggtgaac gagcgcgtga
      601 tcagcagcgc gtcgtcgtcg ccggccagcg tcgcctcggg ggcgccgaac ggctggtaga
      661 gcgcccggat ggcctcgagc tcgtcgcgga cctcctgggc gctctggaac cgctcctcgc
      721 ggcggcgccg gatcgcgcga tcggccacgt cggcgagcgc ctgcggcagc gtcggacaca
      781 gcgcgcggat cggcggcgga tcgcgggtct gcacggcctc ggcgagctcc gcgccgcgca
      841 gcccgtggtg gggcagccgg cccgacagca gctcgtagag cacgaggccg agcgcgtaga
      901 catccgactg catcgtcgcc ggctcgccgc gccacagctc gggcgccatg tacagcggcg
      961 tgccgaggat cgaccccacc gccgtgagcg agccgtccgc ggccgccccg ggcggaccgg
     1021 agccatgggc catggtgacg cgctcggaca gcgagatgtc gccgcccagc gtcgccgcga
     1081 tcgcgtccgg gatcaccgag acaccgctgt gcgccggcac ccgggcgggc cgcaggccgg
     1141 cgtcgagcag cttggcgagc ccgaagtcga gcagcttgac ctcgccggcg tccgacagca
     1201 tcacgttggc gggcttgatg tcgcggtgga gcacgtcgcg ctggtgcgcc gcggccagcc
     1261 cgcgggcgat gccgagcacc agctccagcg cgcgcggcca tgccaccggc ttggccagct
     1321 cgtcgaggct gtggccggcg atcagctcgt aggcgaggta gggccggccg agcacctcgc
     1381 cgacccggta gatgccgacc acgttggggt gctggagccg ggcgatcgcg cgggcctcga
     1441 tcaggaaccg gtcccgcgcg ggcgcgttgg ggtccgccga ggacaggaac ttcagcgcca
     1501 cgggccgatc gagcgtgtca tcgtgtccga ggtacaccgt gcccatgccg ccgcggccga
     1561 gctggcggac cacacggaag ccatcgagct ccgcgggcgg ctcccagccg gggtccgtct
     1621 cgtgctccgg gtcgccgggc atctgcgcaa gctagcacgc gcgccggtcg ctcagcgatg
     1681 ctctcaccga gccgaaacag caagcattgc cagaacttcg acgtcgcacc ttcacccatc
     1741 gtcgcacggg tcgcggggcg acgtggcgcg accgcgtcgc gaccgccgac cggtcgcgtc
     1801 gaacgcgcgg gcgtgcggtg agtgtgcgcg cacggtgttc cgcggccgga tcgacccgcc
     1861 aagctcgcgc ggcgcgtgcg tacggtctcg tttcagcggc aggatcgccg agcgacagca
     1921 cactggagca tcccgccgaa tcagcaatgg gattcgacgg ctcgggcgct ggcgaaatca
     1981 tctcgtccca gcgggcaagg ccacgagctg cgcggcgccg acgcgaccga ggcagatggt
     2041 ccagcggttg ctgttgggtc tctccataga aggaggagag agatggtgac caggaacctg
     2101 ttcgattcgg caactgaccc caacgagtgc cccccggaat ttccgaactg caccgaaccg
     2161 cgtgtcagca gcccggcctc cgcactggtg gaaccgctgc tcgacacgaa caccgagacc
     2221 gcccccaacg agtgcccccc ggaatttccg aactgcaccg aaccgcatgt cggcagcccc
     2281 gacccggtgc tggtcgagcg cgcagcgtga tcaagggatg ggcagctgct gtagcgcggc
     2341 ccggatcgcc ttggcctcgg agagccggga gttgagctgc agcgctttgt cgatgagctg
     2401 gatcccgcgt tctcgggagg cgcgtgacgg ctgcgccgtc gcgagctcga ggtagacctc
     2461 ggcggccgtg cagtaggccg atgcgaggcc cggatcgcgc gtgaccgcct gctcggcctc
     2521 gtcgcgcgcc ttctcgaggg ccggcatcgg cggctgccgc tcatgtgccg cccatcgagc
     2581 ttcggcgagg tcgaggtgcg cggcctcgac gaggagacgg gcggtgaggg gcatcagccc
     2641 cgtggtcttg gtcagcgcgg cgcgtcccgc cgcgatggtg ctcgtggggt tggtgccgct
     2701 gcgcaggaga aacctcgcct cggtgttggc ggcgaccagg cggtggaacg agagcaagct
     2761 caggtcgtgc tggcccgcct cggcgcgatc gagcgcctcc cgcgcggcgg tcagcgccgt
     2821 catcggattg ttttcggttt cgacgaggta gcgggcgagc gccagctccg cttgcgccag
     2881 ggtatcgtgc aacttgtaga agttgcgatc gatcgccagg caacgcgcgc cgacgcgttg
     2941 cgcgctgtcg acggcggttc gcggatcgac tccgatcgcg ttgtcgtggt ccgcgatcgc
     3001 tacatagagg tcgacctggt tggtacaggc ggggaggtac cgcggatcga tgtccgcggc
     3061 gcgctcgtag ctgtgcagtg cctctttgta ctccggcagc gggtccttcc cggccttgtc
     3121 gaacgcggtg gcgagccagc gatgcgctat gccgaggtcg ttgtgggccc gcacgttact
     3181 gggttcgatc gccagcgcct tggcgaactc gtcgagcgcc tggttcagcc agacctcgcc
     3241 gttcccgcca tgaccggtct cgtaggggcc acggtagatg tgtgcgttgc cgagcgcgat
     3301 ccaggtatgc gcgtcctgcg gatcgagctc gaccgcgcgc gcggctgcct gggcgatctg
     3361 ttcgagcaga ggtcgctgat cgccctgccc ggtgagcgac gggattcgaa accgtcgcag
     3421 gagcacgtag gacttcgtcg tgtaggccga ggcgtcttcg ggatcggcgc gcagtgcgcc
     3481 agagtcgatg gcgtcctgcg cgcgatcgag cgactcggcg ggcgaccgct tctgccggaa
     3541 atcgatctcg gcgagctgca accaggcctc tgccgccgcc tggtacagcg atgcgtcgct
     3601 gcgcgcgatg tcgatcgcct tcgcatacaa ctccgctgcg ccctcgatcc ccttgtgtgc
     3661 gtcgtcgtag tctccgcggt cgaatgcccg gaccgatgcg cggtagacag cgtcggcggc
     3721 gagctcgagc gcctcggacg atcctgccgc ctgatccgcg accccgcgcg cggcacgctc
     3781 ggcggacgcg aagtcgccgc tgtacagcgc gatgcgcgcc tccagcagcg cggcctcgtc
     3841 gcccgaggcg cgactctgtc gaagctcgtc cagcgccggc gtgagatact gccgcgcaag
     3901 atcctgctgt cgctcggcga gccacgcttt gttgtcgccc tggctgcgca ggtcctcgag
     3961 ggcgcgacga tagagctcgc cgagcgcgcg gccgcgcgcc gcatgcgact ccggcgtctg
     4021 ctgcccgcct tcggcggcgt gggccagctc atccgccgcc tcgcgccact cgtggaacgc
     4081 gaggtggccg cggccgagcg ccgcgtggac gagcgcatcg ccgcactcgc cgaggtcgtg
     4141 atgggtcgcg gcgatcgcgc gcatccggtc gcggacgcgc gcgcgatcgc gccgcatatc
     4201 gtgcagtgga tgcaggtacg cattttgcag cgtggcctcg atctcgctgg tctggcggcc
     4261 gagcatct
//
LOCUS       DASVOR010000190         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_60325,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000190 DASVOR010000000
VERSION     DASVOR010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60325"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VF516_04275"
     CDS             complement(<1..546)
                     /locus_tag="VF516_04275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106918.1"
                     /translation="MAVCPTLPSAPCATVVSGGGAIATPRLRSQIGRYAIEGLLGAGG
                     MAVVYLARDPVLGRAVALKVMRVAGDEPGASRLVREGQALARISHPNVISVYEVGRET
                     DGLIYIAMERIVGATLAAWLETPRAPHEILQVFAAAGRGLSAAHAAGLVHRDFKPENV
                     RVGADGRVCVLDFGRARASRDA"
     gene            complement(731..1672)
                     /locus_tag="VF516_04280"
     CDS             complement(731..1672)
                     /locus_tag="VF516_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106919.1"
                     /translation="MSFITALSLAAAAPLAGCVVHEAAPEAPDTGTLTLPLTQTGPHG
                     EQFRLTNATFDIAGGSGFAQTVTSGLEAEIALAVPPGPTTIQLRDGWTLQQLASSGAF
                     VPVDAVLGSVNPAFVRMLASQPAVAEFDFLVRSVDGTLTIRLGVVNTPRELAGPMFVM
                     QATGGLAAYASARMDFAIFFDLAALDRVTLPDGSKQLAYTAGPAALELYNDALGTLAP
                     VLKADQTGGFLQYTVTAKPDGTIELAGSMSGQTSVLSFGPHAIDLAPPPLDADGFPAD
                     AFFLDFSLPFTLDATATDGAGGTLSGILRPRHLLPSP"
     gene            2016..2738
                     /locus_tag="VF516_04285"
     CDS             2016..2738
                     /locus_tag="VF516_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106920.1"
                     /translation="MLARGLLVHPPIVKARLLISAAAAGAIAVAGAAHADPESLNPDA
                     FDLSGAIDRPVSPTRGPFEVVVAGGYTQGAGGAGLIGNIEDVAGPGGTVELQLGYRVS
                     PWFSAGVYGAVARFRHGDAIGDGSRAYGATAGVQAVWHTSDTRSIDPWVSVGAGWRGL
                     WVNRVGAPASSMQGIELLRLQLGIDYRLSPRLAVAPVISASLSMFLVETAAMPDDFTS
                     VQDKRLNLYGFAGFLGRFDLGE"
     gene            complement(2760..3509)
                     /locus_tag="VF516_04290"
     CDS             complement(2760..3509)
                     /locus_tag="VF516_04290"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8106921.1"
                     /translation="MTKVAIITGASSGIGAASAVELARRGWSVVINYSRSEDNAKAVA
                     ERCPDALVVRADVADDAQCRALAQAALDKWGRIDALVNNAGTTKFVDHKNLDGLSADD
                     FLAIYRTNVVGAFQMTRACKPALQASRGAIVNVSSVASEVPVGSSIAYGASKAALNMM
                     TRSLARALGPEIRVNAVCPGYVDTPWHHNAHGSERANQIAAHYASMVPVGDFARSDDI
                     ADAIVWLIEGARQVTGEAIFVDGGLHISPPR"
BASE COUNT          475 a         1283 c         1380 g          523 t
ORIGIN      
        1 ggcgtcgcgc gacgcccgcg cccgcccgaa gtcgagcacg cagacccggc cgtcggcgcc
       61 gaccctcacg ttctcgggct tgaagtcgcg gtggaccagc ccggcggcgt gcgcggccga
      121 cagcccgcgg ccggccgcgg cgaacacctg gaggatctcg tgcggcgcgc gcggagtctc
      181 gagccaggcc gcgagcgtcg cgcccacgat ccgctccatc gcgatgtaga tcaacccgtc
      241 ggtctcgcgg ccgacctcgt agaccgagat cacgttgggg tgcgagatcc gcgcgagcgc
      301 ctggccctcg cgcaccaggc ggctcgcgcc gggctcgtcg ccagcgaccc gcatcacctt
      361 gagcgcgacc gcgcggccga gcaccggatc gcgcgcgagg tacaccaccg ccatcccgcc
      421 ggcgccgagc agcccctcga tcgcgtagcg cccgatctgg ctgcgcagcc tcggtgtcgc
      481 gatcgcgccg ccgccgctga ccacggtggc gcacggcgcg ctgggcaagg tgggacagac
      541 ggccatcggt gctgcgcggg tgacccgtcc atcgactccc cgccggcctc cccgtccgtc
      601 gatcatcctg gctagaagag tcctggtctc gggcccggtc gcacaggatc ggattccccg
      661 aggaggaacg cgacgatcgg caagcttggc cgggctgaat ggcatgcagc cggcggcatg
      721 gcgcgccgcg ctacggcgag ggcagcaggt gccgcggccg cagtatgccg ctcagcgtcc
      781 cgccggcgcc gtcggtggca gtcgcgtcga gcgtgaacgg cagggagaag tccaggaaga
      841 acgcgtcggc cgggaagccg tcggcgtcga gcggcggcgg ggcgagatcg atcgcgtggg
      901 ggccgaagct cagcacgctg gtctgacccg acatcgagcc ggcgagctcg atcgtcccgt
      961 cgggcttcgc ggtgaccgtg tactgcagga agcctcccgt ctggtcggcc ttgagcaccg
     1021 gcgccagcgt gccgagcgcg tcgttgtaga gctcgagcgc ggccgggccg gcggtgtacg
     1081 cgagctgctt gctcccgtcg gggagcgtca cccggtcgag cgcggcgagg tcgaagaaga
     1141 tcgcgaagtc catccgcgcg ctggcgtagg cggccaggcc tccggtcgcc tgcatgacga
     1201 acatcggccc ggcgagctcg cgcggcgtgt tgaccacgcc gagccggatc gtcagcgtgc
     1261 cgtcgacgct gcggaccagg aagtcgaact cggccaccgc gggctggctg gcgagcatgc
     1321 ggacgaatgc ggggttgacg ctgccgagca cggcatcgac cggcacgaac gcacccgacg
     1381 aggcgagctg ctggagcgtc cagccgtcgc gcagctggat cgtggtgggg ccgggcggca
     1441 cggccagcgc gatctcggcc tccaggccgc tggtcacggt ctgcgcgaag ccgctgccgc
     1501 cggcgatgtc gaacgtcgcg ttggtcagcc ggaactgctc gccgtgcggg ccggtctggg
     1561 tcagcggcag cgtgagcgtg ccggtgtcgg gtgcctcggg cgcggcctcg tggacgacgc
     1621 agccggcgag cggcgccgcg gcggcgagcg agagcgcggt gatgaatgac atgaatgtac
     1681 gcatggttcc ctcctccggc cgcggcgaat tgcaggcgat gtgccgccgc gtcaccggcg
     1741 gtgtgcagga cttcgcggcc cggatactgc tgcgggcagg acccggacac gctgtgtcgt
     1801 cacgtggcag tcaccgtcac tctgctgttg cagtcgcggc agatccggcg cggtcccgcg
     1861 gcgtgacgcg gatgccgcgg tccgcgcgct ccgtcgtgct cgaaccgcac gcctgccggc
     1921 gtcggtgggc cgacccctcg ctgtcagcga ttccggcagg tgccggtttt tgctcgaccg
     1981 gctgcgctcg atgtccgcga catcatgggc cgtgtatgct ggcgcgcggc ttgctcgtgc
     2041 atccgcccat cgtgaaggca cggctcttga tctccgctgc cgccgcgggc gcgatcgcgg
     2101 tcgccggcgc ggcgcacgcc gaccccgagt cgctcaaccc cgacgcgttc gatctcagcg
     2161 gggcgatcga ccggccggta tcgccgaccc gcggcccgtt cgaggtcgtg gtcgccggcg
     2221 gctacaccca gggcgccggc ggcgccgggc tgatcggcaa catcgaggac gtggcggggc
     2281 cgggcggcac cgtggagctc cagctcggct accgggtgag cccctggttc agcgccggcg
     2341 tgtacggcgc ggtcgcgcgg ttccgccacg gcgacgcgat cggcgacggc agccgggcgt
     2401 acggcgccac cgccggggtc caggcggtgt ggcacaccag cgatacccgc tcgatcgacc
     2461 cgtgggtcag cgtcggcgcc ggctggcgcg gcctgtgggt caaccgggtc ggcgccccgg
     2521 cgtcgtcgat gcagggcatc gagctgttgc gcctccagct cggcatcgat taccggctgt
     2581 cgccccggct cgcggtcgcg ccggtgatct cggccagcct gtcgatgttc ctggtcgaga
     2641 ccgcggcgat gccggacgac ttcacctcgg tccaggacaa gcgactcaac ctgtacggct
     2701 tcgccggctt cctgggccgg ttcgatctgg gcgagtaggc ccggcgagca ggccgccgat
     2761 cagcgcggcg ggctgatgtg gaggccgccg tcgacgaaga tcgcctcgcc ggtgacctgg
     2821 cgggcgccct cgatcagcca gacgatcgcg tcggcgatgt cgtcggaccg cgcgaagtcc
     2881 ccgaccggca ccatcgaggc gtagtgcgcc gcgatctggt tggcgcgctc gctgccgtgc
     2941 gcgttgtggt gccacggtgt gtcgacgtag cccgggcaca ccgcgttgac ccggatctcg
     3001 gggccgagcg cgcgggcgag cgagcgcgtc atcatgttga gcgccgcctt ggaggcgccg
     3061 tacgcgatcg acgagccgac cggcacctcg gacgcgaccg acgacacgtt gacgatcgcg
     3121 ccgcggctgg cctggagcgc gggcttgcac gcccgcgtca tctggaacgc gccgaccacg
     3181 ttggtgcggt agatcgcgag gaagtcgtcg gcgctgaggc cgtcgaggtt cttgtgatcg
     3241 acgaacttgg tcgtgccggc gttgttgacc agcgcgtcga tccggcccca cttgtcgagc
     3301 gcggcctggg ccagcgcgcg gcactgcgcg tcgtccgcga cgtcggcgcg gaccacgagg
     3361 gcgtcggggc agcgctcggc gacggccttg gcgttgtcct cgctgcgcga atagttgatg
     3421 acgaccgacc agccacggcg ggcgagctcg acggcgctgg ccgcgccgat acccgacgag
     3481 gctccggtga tgatggcgac cttggtcatg acagctccag gcagccgcag cagcgcgcgg
     3541 cggcgcgagc ctaccgcggc cccgggccgg ccggggagcc ggtcgcgtcg gtcctgtgcc
     3601 ggtcatgtgc cgacatgtga tcatcccggc gatgcgggtc gttcgattca ggacagcagc
     3661 t
//
LOCUS       DASVOR010000191         3198 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_177286,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000191 DASVOR010000000
VERSION     DASVOR010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177286"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3198)
                     /locus_tag="VF516_04295"
     CDS             complement(<1..>3198)
                     /locus_tag="VF516_04295"
                     /inference="COORDINATES: protein motif:HMM:TIGR04020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MupA/Atu3671 family FMN-dependent
                     luciferase-like monooxygenase"
                     /protein_id="HEX8106922.1"
                     /translation="AAAPEVAIAGDDVAYLMYTSGSTGRPKGTAVRHRNVVNFFAGMD
                     ERVGCGASDVVLAVTSVSFDISVLELLWPLTRGAKVVVAGEGLVNNLVPSAGGATRAC
                     SFSLFFFAAASSAARLGAGTPTALPVGRGAEPPVGSSCSNRAGYQLVLDAARFADRHG
                     FEAVWTPERHFHPFGGLYPNPSVMAAALATITSRVALRSGSVVAPLHDAVRLAEEWSV
                     VDNLSNGRVGLAFASGWNSNDFVLAPARFADRKQVMNEQLADFRRLWRGETVRRINGT
                     GQDIDVRIFPPPVQPEPPIWLTSVGSIDTFQRAGAVGANVLTHLLGQSPADLEPKIRA
                     YRETRAAHGHAGPGRVTLMIHAFMQADAGAAKQRARGPFRDYLRSSVELWRTLFAITG
                     QEFPTSGAPEQLEAVIELAIERYFETSGLFGSPETCADLVRELAGAGVDELACLIDFG
                     IEPGEALDSLVSVDELRRAHRDEVADRQHSFTELCARHGVTLLQGTPSLMTAITAEPL
                     ALDALRGARALLVGGEAFPVGLAERLAEALPATRIVNMYGPTETTIWSTTHELDRARD
                     LDGATISIGRPIANTVLQITDTHGRPVPVSVPGELWIGGDGVAVGYLGQPELTAERFV
                     ELAGQGRFYRTGDRTLWRGDGTVDFLGRMDRQVKILGHRVEPDEIESVLSRHPEIESV
                     AVVARSGASGTELVAYVSPARVVATDPGVEGSFVRRWGELWQDTYAGSQASGAEFAGW
                     TSSYDGLPIPPDQMREWLTHTVARIRGLRPRTVADIGVGVGLVLRELAAHTTRYYGVD
                     ISPAALAAAADCLGPDRSLPSHVHLEHSGPEYLASLPARSVDVVVLNSVVQYFPSVGY
                     LRKVVGDAVRVVAPGGAIYVGDVRALEMLPEFHTAVQLHRAQPLQTIEELRSAVARQL
                     QDERELCISPQFFRGLAAELPEIREVRIELKRGRADNELTQFRYDVSLIVGEPGGAPR
                     ALPRIDFVECRGGLDELRERLAASAAPLLVTGIPNRRLWRVARASQLLREMPHKATAW
                     DLDRGLWEIDDGATIHPEDVLGMASSVAREAQVIVP"
BASE COUNT          447 a         1159 c         1183 g          409 t
ORIGIN      
        1 ggcacgatca cctgggcctc gcgggccacg ctcgacgcca tgccgagcac gtcctcgggg
       61 tggatggtcg cgccgtcgtc gatctcccac agcccgcggt cgagatccca ggccgtggcc
      121 ttgtgcggca tctcgcggag cagctgcgac gcccgggcga cgcgccacag ccggcggttg
      181 ggaatgccgg tgaccagaag cggcgccgcg ctggccgcga gccgctcgcg cagctcgtca
      241 agcccgcccc ggcactcgac aaagtcgatg cgcggcaacg cgcgcggcgc cccgccgggc
      301 tcgccgacga tcagcgacac gtcgtagcgg aactgggtca gctcgttgtc ggcgcggccg
      361 cgcttgagct cgatccgcac ctcgcggatc tcgggcagct ccgccgccag gccgcggaag
      421 aactgcggcg agatgcacag ctcccgctcg tcctggagct ggcgcgcgac cgccgagcgg
      481 agctcctcga tggtctgcag cggctgggcg cggtggagct gcaccgcggt gtggaactcg
      541 ggaagcatct ccagcgcgcg cacgtcgccg acgtagatcg cgccgccggg ggccacaacc
      601 cggaccgcgt cgccgaccac cttgcgtagg tagccgacgc tcgggaagta ctggacgacc
      661 gagttgagca cgacgacgtc gacgctgcgc gccggcaggc tggcgaggta ctccgggccg
      721 gagtgctcca ggtggacgtg cgacggcagc gaacgatccg ggccgaggca gtccgccgcc
      781 gcggcgagcg ccgccggcga gatgtcgacg ccgtagtacc gcgtggtgtg cgcggcgagc
      841 tcgcgcagga ccaggccgac gccgaccccg atgtccgcga ccgtgcgcgg ccgcagcccg
      901 cggatccggg ccacggtgtg ggtgagccac tcgcgcatct ggtccggagg gatcggcagg
      961 ccgtcgtagc tgctggtcca cccggcgaac tcggcgccgc tcgcctgcga gccggcgtag
     1021 gtgtcctgcc acagctcacc ccagcgccgg acgaagctgc cctcgacacc cggatcggtg
     1081 gcgacgaccc gggccggcga gacgtaggcg accagctcgg tgccgctggc gccgcttcgc
     1141 gcgaccaccg ccaccgactc gatctcggga tggcgcgaca gtacgctctc gatctcgtcg
     1201 ggctcgaccc ggtggccgag gatcttgacc tggcggtcca tgcgcccgag gaagtcgacc
     1261 gtgccgtcgc cgcgccacag cgtgcggtcg ccggtgcggt agaaccgccc ctggccggcg
     1321 agctcgacga agcgctcggc ggtgagctcg ggctggccga ggtagccgac cgcgacaccg
     1381 tcgccgccga tccacagctc cccgggcacg ctgaccggca ccggccggcc gtgcgtgtcc
     1441 gtgatctgca gcacggtgtt ggcgatcggc cggccgatcg agatcgtcgc gccgtcgagg
     1501 tcgcgcgcgc gatcgagctc gtgggtggtc gaccagatcg tcgtctcggt cggaccgtac
     1561 atgttgacga tccgggtcgc cggcagcgcc tcggccagac gctcggcgag cccgaccggg
     1621 aacgcctcgc cgccgaccag cagcgcacgc gccccgcgca gtgcgtcgag cgcgagcggc
     1681 tcggcggtga tcgcggtcat cagcgatggc gtgccctgga gcagggtcac gccgtggcgc
     1741 gcgcacagct cggtgaacga gtgctggcgg tcggcgacct cgtcgcggtg ggcgcggcgg
     1801 agctcgtcga ccgaaaccag gctgtccagc gcctcgccgg gctcgatgcc gaagtcgatc
     1861 aggcacgcca gctcgtcgac ccccgcgccg gccagctctc gcaccaggtc ggcgcaggtc
     1921 tccggcgagc cgaacaggcc cgaggtctcg aagtagcgct cgatcgcgag ctcgatcacg
     1981 gcttcgagct gctccggcgc gccgctggtc gggaactcct ggccggtgat cgcgaacagc
     2041 gtgcgccaca gctcgaccga gctgcgcagg tagtcgcgga acgggccgcg cgcccgctgc
     2101 ttggccgcgc cggcgtcggc ctgcatgaac gcgtggatca tcagggtcac gcgcccggga
     2161 cccgcatggc cgtgagccgc gcgcgtctcg cggtacgcgc ggatcttggg ctccagatcg
     2221 gcggggctct ggccgagcag gtgggtcagc acgttggcgc cgaccgcgcc ggcgcgctgg
     2281 aacgtgtcga tcgatccgac cgaggtcagc cagatcggtg gctcgggctg gaccggcggg
     2341 gggaagatcc gcacgtcgat gtcctggccg gtgccgttga tccgccggac ggtctcgccg
     2401 cgccacagcc ggcggaagtc ggcgagctgc tcgttcatca cctgcttgcg gtcggcgaac
     2461 cgcgccggcg cgagcacgaa gtcgttggag ttccagcccg aggcgaacgc caggccgacc
     2521 cggccgttcg acaggttgtc gaccaccgac cactcctcgg cgaggcgcac cgcatcgtgg
     2581 agcggggcca cgacgctgcc gctgcgcagc gcgacgcggc tcgtgatcgt cgccagcgcg
     2641 gcggccatca ccgatgggtt ggggtacagc ccgccgaacg gatggaagtg gcgctcgggg
     2701 gtccacaccg cctcgaaccc gtggcgatcg gcgaaccgcg cggcgtcgag cacgagctgg
     2761 tagccggcgc ggttggagca gctcgatccc acggggggct ccgccccccg ccccaccggc
     2821 agagccgtcg gggtccccgc ccccagtctc gctgcgctcg acgcggccgc gaagaagaac
     2881 aggctgaagc tgcaggcgcg ggtggcgccg ccggcgctgg gcaccaggtt gttgaccagg
     2941 ccctcgccgg cgaccacgac cttggcgccg cgggtcagcg gccacagcag ctccaggacc
     3001 gagatgtcga acgacacgct ggtcaccgcg agcacgacgt cgctcgcgcc gcacccgacg
     3061 cgctcgtcca tcccggcgaa gaagttgacg acgttgcggt ggcgcaccgc ggtgcccttg
     3121 gggcgcccgg tcgagccaga ggtgtacatc aggtacgcga cgtcgtcgcc ggcgatcgcg
     3181 acctcgggcg cagccgcg
//
LOCUS       DASVOR010000192         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_31052,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000192 DASVOR010000000
VERSION     DASVOR010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31052"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..1684
                     /locus_tag="VF516_04300"
     CDS             101..1684
                     /locus_tag="VF516_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEX8106923.1"
                     /translation="MELPGLTVDRSRSPSVLHFAPGFNVAAPFIDRHVAEGRGGKIAI
                     RSQAGDVAYAELAEQVNRCGNALIELGVAPGQRMVMVLRDGPEFFYLFWGAIKAGIVP
                     VPLNAILRAADYRVILEDSGASAIVYSAELAGEVEPALAPGAPPIALPVDGGPRSLRA
                     LLAGASPRLDPVPAEATAPCFWLYTSGSTGRPKGAVHRHRDMVVTSELFGRGLLGITE
                     TDVIFSAAKLFFAYGLGNSMTFPLWAGATTTLLDARPTARNTLEHIARLRPTLYFGVP
                     TLYASQLQELETAEHDLGSLRLCVSAGEPLPAEVFRRWKQRTGLTILDGLGSTEALNT
                     FLSNRPDDVRPGTSGRPVPGYQVRIVGPDGGELPPGEPGRLQVKGESTAACYWNNPEK
                     TAQTMQGDWLDTGDTYVVDGDGYYRHCGRSDDMLKVGGLWCSPTEIESRLIDHPAVLE
                     AAVVGIPDASGNIKPEAWIVLRTGHAASDALADALMLHCKHHLAPYKFPRRVHFAAEL
                     PKTATGKIQRYLLRSSAGA"
     gene            complement(1685..>1947)
                     /locus_tag="VF516_04305"
     CDS             complement(1685..>1947)
                     /locus_tag="VF516_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106924.1"
                     /translation="ISRCVAMGGCGMFRGALVRLAHAEALHASGDVEAARRAIGHART
                     HLLAIADKIRDPAYRQGFLESAPENARTLALARAWLDDPASA"
BASE COUNT          260 a          742 c          676 g          269 t
ORIGIN      
        1 acgctgagct ggcgcaagtc ggtcgccagc gcctacgcgg cgcgcgcgct gcgcgagctg
       61 gtgggtgccc cggccagcgg cctggaggcg tcgtagccgc atggagctgc cgggcctgac
      121 cgtcgaccgc tcgcggtcac cctcggtctt gcacttcgcg ccgggattca acgtcgccgc
      181 gccgttcatc gatcgccacg tcgcggaggg gcgcggcggc aagatcgcga ttcgcagcca
      241 ggccggcgac gtcgcgtacg ccgagctcgc cgagcaggtc aaccgctgcg gcaacgcgct
      301 gatcgagctc ggtgtcgcgc ccggccagcg catggtgatg gtgctgcgcg acggcccgga
      361 gttcttctac ctgttctggg gcgcgatcaa ggccggcatc gtcccggtgc cgctcaacgc
      421 gatcctgcgc gcggccgact accgcgtcat cctcgaggat tcgggcgcat ccgccatcgt
      481 ctattccgcc gagctcgccg gcgaggtcga gcccgcgctg gcgcccggcg ctccgccgat
      541 cgcgctgccg gtcgatggcg gcccgcgcag cctgcgcgcg ctgctcgccg gtgcatcgcc
      601 gaggctcgat ccggtgccgg ccgaggccac cgcgccgtgc ttctggctct acacctcggg
      661 gtcgaccggc cggcccaagg gtgccgtcca ccgccaccgc gacatggtgg tcaccagcga
      721 gctgtttggc cgcggcctgc tcggcatcac cgagaccgac gtcatcttct ccgcggccaa
      781 gctgttcttc gcctacggcc tgggcaactc gatgacgttc ccgctgtggg ccggcgccac
      841 caccacgctg ctcgacgcgc ggccgaccgc gcgcaacacg ctcgagcaca tcgcgcggct
      901 gcgtccgacg ctgtacttcg gcgtgccgac gctgtacgcc tcgcagctcc aggagctcga
      961 gacggcagag cacgacctcg gctcgctgcg gctgtgcgtg tccgccggcg agccgctgcc
     1021 ggccgaggtg ttccggcgct ggaagcagcg caccgggctc acgatcctcg acggcctggg
     1081 ctcgaccgag gcgctcaaca cgttcctgtc caaccgcccc gatgacgtgc gcccgggcac
     1141 cagcggccgg ccggtccccg gctaccaggt ccggatcgtc ggccccgacg gcggcgagct
     1201 gccgccgggc gagccgggcc ggctccaggt caagggcgag tcgaccgcgg cctgctactg
     1261 gaacaacccc gagaagaccg cccagaccat gcagggcgac tggctcgaca ccggcgacac
     1321 ctacgtcgtc gacggcgacg gctactaccg ccactgcggc cgcagcgacg acatgctcaa
     1381 ggtcggcggc ctgtggtgct cgccgaccga gatcgagtcc cgcctgatcg accatcccgc
     1441 ggtgctcgag gccgcggtgg tcgggatccc cgacgcgagc ggcaacatca agcccgaggc
     1501 ctggatcgtg ctgcgcaccg gccacgcggc gtcggacgcg ctcgccgacg cgctgatgct
     1561 gcactgcaag caccacctcg cgccctacaa gttcccgcgc cgggtccact tcgccgccga
     1621 gctgcccaag accgccaccg gcaagatcca gcgctacctg ctgcgcagct ccgccggggc
     1681 gtagctatgc gctcgccgga tcgtccagcc aggcgcgcgc gagcgccagg gtcctggcat
     1741 tctcgggcgc gctctcgagg aagccctgcc ggtaggcggg gtcgcggatc ttgtcggcga
     1801 tcgcgagcag atgcgtccgc gcatggccga tggcgcgccg cgcggcctcg acgtcgccgc
     1861 tcgcgtggag cgcctcggcg tgagcgaggc ggaccagcgc accgcggaac atgccgcatc
     1921 cgcccatggc gacgcagcgc gagatcg
//
LOCUS       DASVOR010000193         3343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_352681,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000193 DASVOR010000000
VERSION     DASVOR010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3343
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352681"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VF516_04310"
     CDS             <1..444
                     /locus_tag="VF516_04310"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8106925.1"
                     /translation="LLQFVRTGTIQRAPVDPAALVSGAASSVAGDVTVDATGAPATWS
                     LDAGRIRQVVLNLIDNAVAAGPPVSVTVRRERGRLILEVADRGPGVPEPDRERIFEPF
                     FTGKTQGTGLGLAICRRVVELHGGTIAVYPNPGGGAMFRAEIPES"
     gene            complement(441..1493)
                     /locus_tag="VF516_04315"
     CDS             complement(441..1493)
                     /locus_tag="VF516_04315"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HEX8106926.1"
                     /translation="MSNILVTGGSGFIGSHCILQLLAAGHQVRTTVRSRKREADVRAM
                     LKEGGAEPGDRLSFAVADLEADAGWPEAVAGCEYVLHVASPFPAGVPRNEDELIVPAR
                     EGALRVLRASRDAGVKRVVLTSSFAAIGYGHDPQPAPFDETRWTDLGDHHVRPYAKSK
                     TLAERAAWEFIATEGKDLELSVVNPVGVFGPVLGADYSTSILLVQRLMDGALPGCPKL
                     CFGVVDVRDVADLHLRAMTNPAARGERFLAVAGDFMMVRDIARVLKSRMGAQARRVPT
                     RQLPNWLIRIAALRDPAVRQILPELGKVKNATHAKAQRVLGWTPRSNEDALVATAESL
                     VRLGLLKDSARTSTGF"
     gene            1581..2915
                     /locus_tag="VF516_04320"
     CDS             1581..2915
                     /locus_tag="VF516_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8106927.1"
                     /translation="MARILVADDEPGLREFITDSLELDAHTVVAAKDGREAARLLDER
                     GFDLVLTDLKMPGLDGMAILRKVRAEQPEVEVIMMTAHGTVDNAVEAMKLGAFEYLQK
                     PLSGPDELRLLVGRALERRGLRDRIDGDERRGERAGPPLTYGDPAMRPVVEAIEKVAR
                     TSATVLLLGESGTGKEVAAREIHTRSPRAARPFMAINCAALSEQLLESELFGHEKGAF
                     TGATERQRGRLELADGGTFFLDEVGELKPELQAKLLRVLQERRFERVGGTRTLEVDVR
                     WIAATNRDLRTMIDDGRFREDLYHRLAVVPIKLPPLRDRRLDLLPLARALLERIARDL
                     KRSVPRLTPSAASRLESASWRGNVRELANTLERAAILADGDAIDASHIWLEDGATRPI
                     PKTDEVRPLAELEREAILHALDVVGGNRKRAAELLGIGERTLYDRLKKYDDA"
     gene            complement(3124..>3343)
                     /locus_tag="VF516_04325"
     CDS             complement(3124..>3343)
                     /locus_tag="VF516_04325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106928.1"
                     /translation="GPAPPLAAAADDGAAVPFAAAAVVADHRSPVLDGHAVVAAVFAS
                     VMVGQGDTTREAESECGQGEKLEHHALG"
BASE COUNT          479 a         1256 c         1170 g          438 t
ORIGIN      
        1 ctcctgcagt tcgtccgcac cggcacgatc cagcgcgccc cggtcgaccc cgccgcgctc
       61 gtctcgggcg ccgcatcgag cgtggccggc gacgtgaccg tcgacgccac cggcgcgccc
      121 gccacctggt cgctcgacgc cggccggatc cgccaggtcg tgctcaacct gatcgacaac
      181 gcggtcgccg ccggcccgcc ggtcagcgtc accgtgcgcc gggagcgcgg ccggctgatc
      241 ctcgaggtcg ccgaccgcgg cccgggcgtc cccgagcccg atcgcgagcg gatcttcgag
      301 ccgttcttca ccggcaagac ccagggcacg ggcctcggcc tggcgatctg ccgccgcgtc
      361 gtcgagctcc acggcggaac gatcgccgtg tacccgaacc cgggtggcgg cgccatgttc
      421 cgcgccgaga tccccgagag ctagaagccc gtcgaggtcc ttgcgctgtc cttgagcagg
      481 ccgagccgca ccaggctctc ggcggtggcg accagcgcgt cctcgttcga gcgcggcgtc
      541 caccccagga cgcgctgggc ctttgcgtgg gtcgcgttct tcaccttgcc gagctcgggc
      601 aggatctgcc gcaccgccgg atcgcgcagc gcggcgatgc ggatcagcca gttcggcaac
      661 tgcctcgtcg gcacgcgccg ggcctgtgcg cccatgcgcg acttcagcac cctggcgatg
      721 tcgcgcacca tcatgaagtc gccggcgacg gcgaggaacc gctcgcccct ggcagcgggg
      781 ttcgtcatcg cgcgcaggtg caggtcggcg acatcgcgga catcgaccac gccgaaacac
      841 agcttcgggc agcccggcag ggcgccgtcc atcaggcgct gcaccagcag gatcgacgtc
      901 gagtagtcgg cgcccaggac cgggccgaac acacccaccg ggttgacgac cgagagctcg
      961 aggtccttgc cctcggtggc gatgaactcc cacgcggcgc gctcggccag cgtcttggac
     1021 ttcgcatagg gcctcacgtg gtggtcgccg aggtccgtcc agcgggtctc gtcgaacggc
     1081 gccggctgcg gatcgtggcc atagccgatc gccgcgaacg acgacgtcag caccacgcgc
     1141 ttgacgccgg cgtcccgcga ggcgcgcagc acgcgaagcg cgccctcgcg cgcgggcacg
     1201 atcagctcgt cctcgttcct ggggacgccc gcggggaacg gcgatgccac gtgcagcacg
     1261 tactcgcaac cggcgacggc ctcgggccag cctgcgtcgg cctcgaggtc ggcgacggcg
     1321 aacgacaggc gatccccggg ctcggcgccg ccttccttca acatggcgcg cacgtcggcc
     1381 tctcgcttcc ggctccgcac cgtggtgcgc acctgatggc ccgcggcgag aagctgcagg
     1441 atgcagtggc tgccgatgaa acccgacccg ccggtcacga ggatgttgct catcgggtct
     1501 gttctacccc gcttcgaccg ggagctacga gccgcaagcc gcgagtctcc accgaccgcg
     1561 tagggcatac ttctgccgcc atggcacgca tcctggtcgc cgacgacgaa cccggcctgc
     1621 gcgagttcat caccgattcg ctggagctcg acgctcacac cgtggtcgcc gccaaggacg
     1681 gccgcgaggc cgccaggctg ctcgacgagc gcggcttcga cctcgtgctc accgatctca
     1741 agatgccggg gctcgacggc atggcgatcc tgcgcaaggt ccgcgccgag cagcccgagg
     1801 tcgaggtcat catgatgacg gcgcacggca cggtcgacaa cgcggtcgag gcgatgaagc
     1861 tcggcgcgtt cgagtacctc cagaagccgc tgtcggggcc cgacgagctc cgcctgctgg
     1921 tcgggcgcgc gctggagcgc cgcggcctgc gcgaccggat cgacggcgac gagcgccgcg
     1981 gcgagcgcgc cggcccgccg ctgacctacg gcgatccggc gatgcggccg gtggtcgagg
     2041 cgatcgagaa ggtcgcccgg acctcggcga ccgtgctgct gctcggcgag agcggcaccg
     2101 gcaaggaggt cgcggcccgc gagatccaca cccgcagccc gcgcgccgcg cggccgttca
     2161 tggcgatcaa ctgcgccgcg ctcagcgagc agctgctgga gagcgagctg ttcggccacg
     2221 agaagggcgc gttcaccggc gcgaccgagc gccagcgcgg ccggctcgag ctcgccgacg
     2281 gcggcacgtt cttcctcgac gaggtcggcg agctcaagcc cgagctccag gccaagctgc
     2341 tccgcgtgct gcaggagcgc cggttcgagc gggtcggcgg cacccgcacg ctcgaggtcg
     2401 acgtccgctg gatcgcggcg accaaccgcg acctgcgcac gatgatcgac gacggccggt
     2461 tccgcgagga cctctaccac cggctcgcgg tggtcccgat caagctgccg ccgctgcgcg
     2521 accgccggct cgacctcctg ccgctcgccc gcgccctgct cgagcggatc gcgcgcgacc
     2581 tgaagcgcag cgtgccgcgg ctgaccccga gcgcggcgag ccggctggag agcgcgagct
     2641 ggcgcggcaa cgtgcgcgag ctggccaaca cgctggagcg cgccgcgatc ctcgccgatg
     2701 gcgacgcgat cgacgccagc cacatctggc tggaggacgg cgcgacccgg ccgatcccca
     2761 agaccgacga ggtccgcccg ctcgccgagc tggagcgcga ggcgatcctg cacgcgctcg
     2821 acgtcgtcgg cggcaaccgc aagcgcgccg ccgagctgct cgggatcggc gagcgcacgc
     2881 tgtacgaccg gctcaagaag tacgacgacg cgtgagccgg agcggccgcg ctgcgtcggt
     2941 ctccgtcgtc ggcgcagctt gcctgctcgc cctcacctcg cctccatgtc gcaccctggg
     3001 ccgaggcatt ccgccccgac tgcggaatcc gcaggaccgt gaactgcggg ttctgcagcc
     3061 gaccggccaa cggtcccgca acccgatgaa attgctgggc gcaaatgcgg cacggcgggt
     3121 gctttatccg agggcatgat gctcaagctt ctccccctga ccgcactcac tctcggcctc
     3181 tcgagtggtg tcgccctggc cgaccatcac ggaggcgaat accgcggcga ccaccgcgtg
     3241 gccgtcgagc accgggctcc ggtggtccgc gaccacggcc gcggcggcga atggcacggc
     3301 ggcgccgtcg tccgcggcgg cggcgagtgg cggggccgga cct
//
LOCUS       DASVOR010000194         7631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_118855,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000194 DASVOR010000000
VERSION     DASVOR010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7631
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118855"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VF516_04330"
     CDS             <1..434
                     /locus_tag="VF516_04330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106929.1"
                     /translation="AGVALVVLRSTPGAEDRVAIKGVGEVALELVRERAGAISYDARR
                     YAPGDRWKVVISCPPAAPAPNPTPPAAPMMAIDVSVADGVTVDHPLAATRIACGNRVV
                     VPGAFTVTGDRVNRVCARIATVAGPDRDAGTACVTLIPETQ"
     gene            637..4143
                     /locus_tag="VF516_04335"
     CDS             637..4143
                     /locus_tag="VF516_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8106930.1"
                     /translation="MRSRSDGDGSDGPDAARTLAGPTQTSDSRPSIVKRGETDHEPES
                     GSPPPALPIQFRDPRRYEFLGEHGRGGLGRVSRAHDRELGRDVAIKELLSRGTANEVR
                     FLREVLITARLEHPSIVPIHEAGRWSDGTPFYAMKLVAGRPLRELLAERTTVAERIGL
                     LHHVIAVADAIAYAHGRNIIHRDLKPANVIVGDFGETIVIDWGLAKDLSISEVSGGES
                     GSIRILAGAAGSIGPAGSTGSTGSIGLAGSAVAGDVTSAGSVMGTPAYMAPEQRRGEP
                     VDRRADVYAIGLMLWELVVPHRAPPARLPLRHRALRRAGIDEDLATIIEKALDPDPER
                     RYPDAGALAADLKAFKSGARIAARSYSLFAMLAHWTRRHRALALSAVAALAVAITGSL
                     VYVRNIAAERDRADLSNEAARRAQAVAETSFDELTLKHAELLLTTDPSAAIDALARYR
                     GSEVERADQIRAHAAGLGVALVRAVPHTDNVAWAHGAPDGSILSISLDGTIARTTRDG
                     ASSVVSRGASRQGISAYSPSRHLLAYVCNPSDLCLFDVEHGAAFGVAPVLRNASFEGV
                     SFSPDGMLLAVISHEAVLRILDITDPARPALRFTRTVEGGLDIEFAGDDMVVVGRHGG
                     IDFVRMDGAIERFSLPAILRWEVDTRRHELALGTSDGEGLVVSGFPLHVAAQAGLCHG
                     PTADLQFVPGRRSVAYACRDGAIGTWDLESNTVLQRARLEGHADLIAASAAGDYVIAA
                     GGNGAVTVLDLTTDLIATYKGHGFRLTTLTPPGPDQSFLISGDVRGGLRAWPLPRRLV
                     RVAATSSSSFHGAIFDDASATVTATTWQPALTVFSPATGVRSVEPHANENIFLARSIT
                     GRTFATYGLQGDTVEVWASPAMTRTRVIRTGHGSLSQLAFVAESEDFLTSGQDGRLVR
                     WTPAGDGTPVVQFGQPIDHFAHAAATDAIVFSTVAGALWRTSSGGPVAALRDGGPRVN
                     RLVALPDRQTVLAGTEDGDVIAIDTRSWQQHVLLHGGSVREIAFTADGRTIAVASNDG
                     TIHVGTRRGDAPLAAGMTWTALPLRARHITLVPDGLLVASCTDGTIWLYTPASRRWLC
                     LPTGTVDLGRTAATADGKVAIALDFEGRLLWIDLEAARQLLQGRAIEAIKTPHQGTRS
                     HEEVQEEVQDQGTTGSQARPPS"
     gene            4076..4276
                     /locus_tag="VF516_04340"
     CDS             4076..4276
                     /locus_tag="VF516_04340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106931.1"
                     /translation="MKKSKKKSKIKAPPARKLVLRRESIAQLTRAQLDHVAGADQVED
                     GCSYRPQSCAQSNALDVSCVTV"
     gene            4340..4510
                     /locus_tag="VF516_04345"
     CDS             4340..4510
                     /locus_tag="VF516_04345"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HEX8106932.1"
                     /translation="MKKSTPRRLPRKLVLRKEPIAVLTPPQLGNVPGGGSQLRACTVW
                     SNDEGVCGTTEA"
     gene            complement(4520..5110)
                     /locus_tag="VF516_04350"
     CDS             complement(4520..5110)
                     /locus_tag="VF516_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106933.1"
                     /translation="MKLVEAESTGPVEGDSALVTPPRPPRRRVSVSLVFTLTVLVGTV
                     VTIYAMFPARHNLLMTEAIAHHRDPGAAGAAWDLVSPARDALRAWMIGAIGKDAPLPG
                     ESAAVIGARRLDILNRTAALIRLRIGDDEVTSLVQRARGIAPEDAERVERSEMGGEAP
                     RGIDVTLRAVAWRRGRFTVVAVGADATAARWRAVLR"
     gene            complement(5107..5502)
                     /locus_tag="VF516_04355"
     CDS             complement(5107..5502)
                     /locus_tag="VF516_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106934.1"
                     /translation="MRFGPWYPLADARDLAPAAEGVLQLRLAAGLLDYPRGKSAMVHY
                     AHATDVRAAALAWAAAYGAEGIVCRHLIEDEGTAPAALYARLIDEFVRRFGAPPRRPG
                     SEPGSKPGSKGDSAPGAAGTGGGRGSGAP"
     gene            5524..6627
                     /locus_tag="VF516_04360"
     CDS             5524..6627
                     /locus_tag="VF516_04360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEX8106935.1"
                     /translation="MSSEREVRGPAAGAGAGDAQLALDLRPDLASDPDLASDLAPDLR
                     PPLTRRSRRSRRSGARRRRADPLIGASPAPDLIDFGRTGEPDRVVCGDALDELTALGD
                     GALAAIYIDPPFGTGSVRRGRRHAYADHADDPEGFVAWLSPYLEHSRRVLAATGSLFV
                     HLDYRSVHYVKVALDRLFSRNGFVNEIIWCYAVGGKSRRGFGRKHDTILWYARTRNWA
                     FYADAVRVPRRGGSHMRVVDGVQEKTDRRTGRIYRYPISAGKVPEDWWTDVETLNHSD
                     RERTGWPSQKPERLVERILRAVTVEGDRVADWFAGSGTTAAVAQRLGRGFVTVDRAAA
                     AVEVCVQRLTRQGRRLAAAGAPPAPIRVVRGNG"
     gene            6739..>7631
                     /locus_tag="VF516_04365"
     CDS             6739..>7631
                     /locus_tag="VF516_04365"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX8106936.1"
                     /translation="MKLEAWLVTDVGVVREHNEDSAYMEPANGFFIVADGMGGHAAGE
                     VASAMAVETVRKTLEAARKEIDSFKRSPTDAGRRGIVQLLQSAVLSAHQAVFQRGQHE
                     ADKAGMGTTLDVVLIAGPEAFVAHVGDSRTYLVRDGRSSQITTDHTVAEVLVIEGKLT
                     IEEAQVSPLRTILVNAIGVSADVGVEMAHVTLKRGDRLLLCSDGLHDYFPIEEEIAQK
                     LSADAPGDALKDMVELAKTRGGHDNITGVAVQVTDVIEAIPDRLDGEMTQPVDLPGSN
                     PFASDEPTETAVLDATGTPRVT"
BASE COUNT         1075 a         2877 c         2672 g         1007 t
ORIGIN      
        1 cggccggggt ggcgctggtc gtgctgcgat cgacgccggg cgccgaggac cgcgtcgcga
       61 tcaagggcgt cggcgaggtc gcgctggagc tggtgcgcga gcgcgccggc gcgatctcgt
      121 acgacgcgcg ccgctacgcg cccggcgatc gctggaaggt cgtgatcagc tgcccgcccg
      181 ccgccccggc gccgaacccg acgccgccgg cggcgccgat gatggcgatc gacgtctcgg
      241 tcgccgacgg cgtcaccgtc gaccatccgc tcgccgcgac ccggatcgcg tgcggcaacc
      301 gggtcgtggt gccgggcgcg ttcaccgtca ccggcgaccg ggtcaaccgg gtgtgcgcgc
      361 ggatcgcgac ggtcgccggc cccgatcgcg atgccggcac ggcgtgcgtg acgctgatcc
      421 ccgagacaca gtgacccacc cggtcacccc cctggagcat cctgcggcgc ggcacgacac
      481 gcacccaccc ggcatgcgct ccgttatgtc gaactatcgt tcagaatagg cgtctggata
      541 ctgcgctgtt cagacatgta ttgtacggca cagggaaagg ctgcggaggc cggttcgact
      601 agcaacggtg cacagtgctc cgatacgttg cccgtgatgc ggtcccgcag cgacggcgac
      661 ggttctgacg gtccggacgc cgcacggacg ctggccggcc cgacccagac cagcgattcg
      721 cggccgagca tcgtcaagcg cggcgagacg gaccacgagc cagaatcggg ctcaccaccg
      781 cccgcactgc cgatccagtt ccgcgatccg cggcgctacg agttcctggg cgagcacggc
      841 cgcggcggac tgggccgggt gtcgcgcgcc cacgatcgcg agctcggccg cgacgtcgcg
      901 atcaaggagc tgctgtcgcg cggcaccgcc aacgaggtcc gcttcctccg cgaggtgctg
      961 atcaccgcgc ggctggagca ccccagcatc gtgccgatcc acgaggccgg ccggtggtcc
     1021 gacggcaccc cgttctacgc gatgaagctg gtcgccgggc gtccgctgcg cgagctgctc
     1081 gccgagcgca ccaccgtggc cgagcgcatc ggcctgctcc accacgtgat cgccgtggcc
     1141 gacgcgatcg cctacgccca cggccgcaac atcatccacc gcgatctcaa gccggccaac
     1201 gtgatcgtcg gcgacttcgg cgagaccatc gtgatcgact ggggtctcgc caaggacctg
     1261 tcgatcagcg aggtgtcggg cggcgagagc ggatccatcc ggatcttggc cggcgcggct
     1321 ggctcgatcg gcccggccgg ctcgaccgga tcgaccggct cgatcggcct ggccggctcg
     1381 gcggtcgccg gcgacgtgac ctcggcgggc agcgtcatgg gaacgccggc gtacatggcc
     1441 cccgaacagc ggcgcggcga gccggtcgac cggcgcgccg acgtctacgc gatcggcctc
     1501 atgctgtggg agctcgtcgt cccccacagg gcgccgcccg cccggctgcc gctccgccac
     1561 cgcgcgctgc gccgcgccgg gatcgacgag gatctcgcga cgatcatcga gaaggcgctc
     1621 gaccccgatc ccgagcgccg ctaccccgac gccggggcgc tcgccgccga cctcaaggcg
     1681 ttcaagtcgg gcgcccggat cgcggcgcgc agctactcgc tgttcgcgat gctcgcgcac
     1741 tggacgcgcc ggcaccgcgc gctcgcgctg tcggcggtcg ccgcgctcgc ggtcgcgatc
     1801 accggcagcc tggtctacgt ccgcaacatc gcggccgagc gcgaccgcgc cgacctgtcc
     1861 aacgaggccg cgcggcgcgc ccaggcggtc gccgagacct cgttcgacga gctcacgctc
     1921 aagcacgccg agctgctgct gaccaccgac ccgtcggccg cgatcgacgc gctcgcccgc
     1981 taccggggca gcgaggtcga gcgcgccgac cagatccggg cgcacgccgc cgggctgggc
     2041 gtcgcgctgg tgcgcgcggt gccccacacc gacaacgtgg cgtgggcgca cggtgccccg
     2101 gacggctcga tcctgtcgat cagcctcgac ggcacgatcg cgcggaccac gcgcgacggg
     2161 gcgtcgagcg tggtgtcgcg cggggcatcg cgccagggca tctcggcgta ctcgccgtcg
     2221 cgccacctgc tggcgtacgt gtgcaacccg tcggacctgt gcctgttcga tgtcgagcac
     2281 ggcgcggcgt tcggcgtcgc ccccgtgctg cgcaacgcca gcttcgaggg cgtgtcgttc
     2341 tcgcccgacg ggatgctgct cgccgtgatc tcgcacgagg cggtgctgcg catcctggac
     2401 atcaccgacc cggcgcggcc ggcgctgcgg ttcacccgca ccgtcgaggg cggcctcgac
     2461 atcgagttcg ccggcgacga catggtcgtg gtcggccgcc acggcgggat cgacttcgtg
     2521 cggatggacg gcgcgatcga gcggttctcg ctgccggcca tcctgcgctg ggaggtcgac
     2581 acccgccggc acgagctcgc gctggggacc agcgacggcg agggactggt cgtcagcggc
     2641 ttcccgctcc acgtcgccgc gcaggccggg ctgtgccacg gcccgaccgc cgatctccag
     2701 ttcgtcccgg gccgccgcag cgtcgcctat gcctgccggg acggcgcgat cgggacctgg
     2761 gatctggaga gcaacacggt cctccagcgc gcccggctcg agggtcacgc cgacctgatc
     2821 gccgcgagcg cggccggcga ctacgtgatc gcggccggcg gcaacggcgc ggtcaccgtg
     2881 ctcgacctga cgaccgatct gatcgcgacc tacaagggcc acggcttccg gctgaccacg
     2941 ctcacgccgc cgggccccga ccagtcattc ctgatctccg gcgacgtccg cggcgggctg
     3001 cgcgcctggc cgctgccgcg tcgcctcgtg cgggtcgctg cgacctcgag ctcgtcgttc
     3061 catggcgcga tcttcgacga cgcgtccgcc acggtcacgg cgacgacctg gcagcccgcg
     3121 ctcacggtgt tctcgccggc gaccggcgtc cggtcggtcg agccgcacgc gaacgagaac
     3181 atcttcctgg cgcggtcgat caccggccgg acgttcgcga cctacggcct gcagggggac
     3241 acggtcgagg tctgggcgtc tcccgcgatg acgcggaccc gggtgatccg gaccgggcat
     3301 ggctcgctct cgcagctcgc gttcgtcgcc gagagcgagg acttcctcac gtcgggccag
     3361 gacggccggc tggtgcgctg gaccccggcc ggcgacggca cgccggtggt ccagttcggc
     3421 cagccgatcg accacttcgc gcacgccgcg gcgaccgacg cgatcgtgtt cagcaccgtg
     3481 gccggtgcgc tgtggcgaac gagctccggc ggcccggtgg ccgcgctgcg cgacggcggg
     3541 ccccgggtca accggctggt cgcgctcccc gaccggcaga ccgtgctggc cggaaccgag
     3601 gacggcgacg tgatcgcgat cgatacccgg tcgtggcagc agcacgtcct gctgcacggc
     3661 ggctcggtgc gcgagatcgc gttcaccgcc gacggccgga cgatcgcggt ggcgagcaac
     3721 gacggcacga tccacgtcgg cacgcggcgc ggcgacgcac cgctggctgc gggcatgacc
     3781 tggacggcgc tgccgctgcg cgcgcgccac atcacgctgg tgcccgacgg gctgctcgtc
     3841 gcgtcgtgca ccgacggcac gatctggctg tacacccccg cctcgcggcg ctggctgtgc
     3901 ctgccgaccg gcacggtcga cctcggccgg accgcggcga ccgccgacgg caaggtcgcc
     3961 atcgccctcg acttcgaggg ccgcctcctg tggatcgacc tcgaagccgc ccgccagctc
     4021 ctccagggca gggcgatcga ggccatcaag acacctcacc aaggaacccg ctcccatgaa
     4081 gaagtccaag aagaagtcca agatcaaggc accaccggct cgcaagctcg tcctccgtcg
     4141 tgagtcgatc gcgcagctga cccgcgcaca gctcgaccac gtcgcggggg ccgatcaggt
     4201 cgaagacggc tgctcgtacc gcccgcagag ctgcgcccag tcgaacgccc tcgacgtctc
     4261 gtgcgtcacc gtgtgaatcc ctgcacgccc acccgccaca ccgcgatccc accaccctca
     4321 cggaccacgg agcccgatca tgaagaagtc cacgcccagg cgcctgcccc gcaagctcgt
     4381 cctccgcaag gagccgatcg ccgtgctcac cccgccgcag ctcggcaacg tccccggggg
     4441 cggctcgcag ctgcgcgcct gcacggtctg gtcgaacgac gaaggggtgt gcggcaccac
     4501 ggaggcgtga cgcggccggt cagcgcagca cggcgcgcca gcgcgcggcc gtggcgtcgg
     4561 cccccaccgc gaccacggtg aaccggccgc gccgccacgc caccgcgcgc agcgtgacgt
     4621 cgatcccccg gggggcttcg ccccccatct cgctacgctc gacccgctcg gcgtcctcgg
     4681 gcgcaatgcc ccgcgcgcgc tgcaccaggg aggtcacctc gtcgtcgccg atccgcagcc
     4741 ggatcagcgc cgccgtgcgg ttcaggatgt cgagccggcg cgcaccgatc acggcggcgg
     4801 actcgcccgg cagcggcgcg tccttgccga tcgcaccgat catccaggcc cgcagggcgt
     4861 cgcgggccgg ggacaccaga tcccacgccg cgccggccgc gcccgggtcg cgatggtgcg
     4921 cgatcgcctc ggtcatcagc aggttgtggc gggcggggaa catcgcgtag atcgtcacca
     4981 ccgtgccgac cagcacggtg agcgtgaaca ccagcgagac cgagacccgg cggcgcggcg
     5041 ggcgcggcgg cgtcaccagg gcggagtcgc cctcgaccgg accggtggac tcggcctcga
     5101 ccagcttcat ggcgcaccgg acccacggcc gcccccggtg ccggccgctc ccggcgcgct
     5161 gtcacccttg ctccctggct tgctccctgg ctcgctccct ggccgccggg gcggggcgcc
     5221 aaaccggcga acgaactcgt cgatcaagcg ggcgtacagc gccgccgggg ccgtgccctc
     5281 gtcctcgatc aggtgccggc acacgatgcc ctcggcgccg tacgccgccg cccacgcgag
     5341 cgccgcggcg cgcacgtcgg tggcgtgggc atagtggacc atcgcgctct tgccgcgcgg
     5401 gtagtcgagc aggccggcgg cgagccggag ctgcagcacg ccctcggccg cgggcgcgag
     5461 atcccgggca tcggcgagcg ggtaccacgg accgaaccgc atgacccata gtagtgtgac
     5521 cctgtgtcga gcgaacgcga agtccgcggg ccggcggccg gcgccggcgc cggtgacgcc
     5581 cagctggcgc tcgatcttcg ccccgatctc gcctccgatc ccgatctcgc ctccgatctc
     5641 gcccccgatc tccgcccacc gctgacccgg cgctcccggc gctcccggcg ctccggggct
     5701 cggcgccgcc gtgcggatcc gctcatcggc gcctccccgg cgcccgacct gatcgacttc
     5761 gggcgcaccg gggagcctga ccgggtggtg tgcggggatg cgctcgatga gctcacggcg
     5821 ctcggcgacg gcgcgctcgc cgcgatctac atcgacccgc cgttcggcac cggcagcgtc
     5881 cggcgcggcc gccggcatgc atacgccgac cacgccgatg accccgaggg gttcgtggcc
     5941 tggctgtcgc cttacctgga gcacagccgc cgggtgctcg ccgccaccgg ctcgctgttc
     6001 gtccacctgg actaccgctc ggtccactac gtcaaggtcg cactggaccg actgttcagt
     6061 cgcaacggat tcgtcaatga aatcatatgg tgctacgcgg tcggaggcaa gagccggcgc
     6121 ggctttggcc gcaagcacga cacgatcctg tggtacgcac gcaccaggaa ctgggcgttt
     6181 tacgccgatg ccgtacgagt cccacgcaga ggtggctctc acatgcgcgt ggtcgacggc
     6241 gtgcaagaga agaccgatcg gaggaccggg cggatctacc gctacccgat ctccgccggc
     6301 aaggtgccgg aagactggtg gaccgatgtc gagacactca accactcgga ccgtgagcgc
     6361 acaggctggc cgtcgcagaa gcccgagcgg ctggtcgagc gtatcctgcg ggcggtcacg
     6421 gtcgaaggcg accgggtcgc ggactggttc gccgggtccg ggaccacggc ggcggtcgcc
     6481 cagcggctgg gccggggctt tgtcaccgtg gaccgcgccg ccgcggcggt cgaggtgtgc
     6541 gtccagcggc tcacccggca gggccggcgg ctggccgccg cgggcgcgcc acccgcgccg
     6601 atccgcgtgg tccgcggcaa cggctgaaga caagacgcat ttgggcccga tcgcttgccg
     6661 gtcggcaccc ggcgggtgag aatttccgca gctctaaggc gactgatggc ggccgctccc
     6721 caagacgaag ccacgcgtgt gaagctcgaa gcctggctcg tcaccgacgt cggagtcgtg
     6781 cgcgagcaca acgaggactc ggcctacatg gagccggcga acgggttctt catcgtcgcc
     6841 gacggtatgg ggggtcacgc tgccggggag gtcgcctcgg cgatggcggt cgagaccgtc
     6901 cgcaagaccc tggaggccgc ccgcaaggag atcgacagct tcaagcggtc cccgaccgac
     6961 gccggccggc gcggcatcgt ccagctgctg cagagcgccg tcctgtccgc ccaccaggcg
     7021 gtgttccagc gcggccagca cgaggccgac aaggctggca tgggcaccac gctggatgtc
     7081 gtgctgatcg ccggccccga ggcgttcgtc gcccacgtcg gtgacagccg gacctacctg
     7141 gtccgcgacg gccggtcatc gcagatcacc accgatcaca ccgtcgccga ggtcctggtg
     7201 atcgagggca agctcacgat cgaggaggcc caggtctccc cgctgcgcac catcctggtc
     7261 aacgcgatcg gggtgtcggc cgacgtcggc gtcgagatgg cgcacgtcac gctcaagcgc
     7321 ggcgaccggc tcctcttgtg ctccgacggc ctgcacgact acttcccgat cgaggaggag
     7381 atcgcgcaga agctgtcggc cgacgcgccc ggcgacgcgc tcaaggacat ggtcgagctc
     7441 gccaagaccc gcggcggcca cgacaacatc accggggtcg ccgtgcaggt caccgacgtc
     7501 atcgaggcca tccccgaccg gctcgacggc gagatgaccc agccggtcga cctgccgggg
     7561 tccaacccgt tcgcatccga cgagccgacc gagaccgcgg tgctcgatgc caccgggacg
     7621 ccccgggtga c
//
LOCUS       DASVOR010000195         5524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_469562,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000195 DASVOR010000000
VERSION     DASVOR010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5524
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469562"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1553)
                     /locus_tag="VF516_04370"
     CDS             complement(156..1553)
                     /locus_tag="VF516_04370"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8106937.1"
                     /translation="MRCSRWCLGFAMLAMMLIPAWVPALAGVAHAGPAAAPVILEGAR
                     LIDGTGKPPRDNAALVIEGDKITAVGVTGKLARPKGARVVDLHGRTIMPGLISAHSHA
                     GLVIGAANSADAYTREAVQAAARQYEQYGVTSFVTLGLNRDLVYELRDQQRQGTVPGA
                     SIFTAGRGIGAPGSVPPQPVQPDQVYRAATADEARAQVREMAGHHVDIVKLWVDDNFG
                     KFPKMQPEVYRAVIDECHKQKLRVAAHVFYLADAKALIAAGVDVLAHSVRDQPVDAEL
                     IRSLKAHGAFYIPTLNVDHSFFVFVDQPEVLSDPFFQRATSPEQLQMLQSQAYKDKVA
                     ANPVVPKEKAAFATATKNLKALHDAGVQIAFGTDSGANPERITGWAEHHELELMVAAG
                     LSPMDAIVAATRTSAALIKASDRGTLEPGKRADFVVLAADPTADIRNTRQLVMVWHGG
                     KEIEPRVPAASKPAR"
     gene            2264..2608
                     /locus_tag="VF516_04375"
     CDS             2264..2608
                     /locus_tag="VF516_04375"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX8106938.1"
                     /translation="MSERMAQAGLDPVAGYTPSVETGQRDTPLARDYPLALVSPANHY
                     FLNSIFANVAHQQRRAGPATLMIHPDDAAPRGIAAGDEVRVGNGIRRSGTPPGSLMFL
                     GLDAEISSCSRA"
     gene            2722..3177
                     /locus_tag="VF516_04380"
     CDS             2722..3177
                     /locus_tag="VF516_04380"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX8106939.1"
                     /translation="MPRSVRKVLRVTGFSLVALLVLAQFVPYGRAHTNRPVHAEPSWD
                     GPTTRELARRACFDCHSNETRWPWYSHVAPTSWLVQHDVDEGREILNFSEWNRPYEEA
                     GEAAETVLERTMPPRAYLLLHPEAKLTDVQRRQLADGLNATLGTSRERE"
     gene            complement(3178..3741)
                     /locus_tag="VF516_04385"
     CDS             complement(3178..3741)
                     /locus_tag="VF516_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HEX8106940.1"
                     /translation="MAALVLWPFTSFAQPAASPQRTIDNLNAAIQGEANASHRYKLFA
                     EQADREGNPQVAKLFRATALAESIHEKDDEAVLRAMGLEPRPPVIENVIVGTTRENLE
                     APIKAERNEEEKTYPAMIAEARREHVPAAAKSFAYAMNTEAEHARLFANALANLGHNP
                     PVDYYVGTVSGDTVTKPTGREPYTKVE"
     gene            4009..4158
                     /locus_tag="VF516_04390"
     CDS             4009..4158
                     /locus_tag="VF516_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106941.1"
                     /translation="MTKPMSMSDGIELELPAGRLLVIAPGLAHDVEAVADSAFLLTLA
                     RGAQG"
     gene            4193..5248
                     /locus_tag="VF516_04395"
     CDS             4193..5248
                     /locus_tag="VF516_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106942.1"
                     /translation="MLHPIAPFRLDLTAWALRRRANNEIDRWDGRTYARTIAVNDRAV
                     DLEVTQAGSATRPALHVVARGAAGHAVRATATAVLSRALGVDIDLADFYRMARGDARL
                     ALLVERFRGLKPPRFPTVFEAVVNGIACQQLSLTVGIILLGRLCNLCGPSSSSGAHAF
                     PRPKDIAALTPARLRALGFNRNKARGLLELACAVEGGFDLEGLADLDDEVVVERLVTL
                     RGVGRWTAEYALLRGLGRLAMFPGDDVGARTNLARWMKLRTPLDYDRVRRLAHRWQPY
                     PGFLYFHLLLQSLDETGTLTPAVPASRASSVLARSGPFGPDLGKRDRRVRERAAGTHL
                     GGDPDRFHQLVGRGPGT"
     gene            5294..>5524
                     /locus_tag="VF516_04400"
     CDS             5294..>5524
                     /locus_tag="VF516_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106943.1"
                     /translation="MRDRNCDDLLRLRGQRAVCEHLLRERPERGIRIGCELVPRDGSS
                     RARNWIMASLTVGSEPEARRVALGITRPCSPPG"
BASE COUNT          756 a         1956 c         1919 g          893 t
ORIGIN      
        1 atcgggatca cgacgaccac gatcaccggc accgtggggc tcagcctgat ggtcgcggcg
       61 cgccgggtcc gggtcgcacc caccttgggg ccgaagaccg ccgggctggc gatctccggc
      121 cgcctgtgat cgggcgcgcc atcaccgcct ggcaatcacc tggcgggctt gctcgccgcc
      181 ggcacgcgcg gctcgatctc cttgccgccg tgccacacca tgaccagctg acgggtgttg
      241 cggatgtcgg cggtcgggtc ggccgccagc acgacgaagt cggcccgctt gcccggctcc
      301 agcgtgccgc ggtcgctggc cttgatcagc gccgcgctgg tccgggtggc cgcgacgatc
      361 gcgtccatcg gcgacagccc ggcggccacc atcagctcga gctcgtggtg ctcggcccag
      421 ccggtgatgc gctcggggtt ggcgcctgaa tcggtgccga acgcgatctg cacgccggcg
      481 tcgtgcagcg ccttgaggtt cttcgtcgcg gtggcgaacg ccgccttctc cttgggcacg
      541 accgggttgg ccgcgacctt gtccttgtag gcctggctct gcagcatctg cagctgctcc
      601 gggctcgtcg cgcgctggaa gaacggatcg ctcagcacct cgggctggtc gacgaacacg
      661 aagaagctgt gatcgacgtt gagcgtcggg atgtagaacg cgccgtgcgc cttgagcgac
      721 cggatcagct cggcgtcgac cggctgatcg cgcacgctgt gggccagcac gtcgacgccg
      781 gcggcgatca gcgccttggc gtcggcgagg tagaacacgt gggcggcgac ccgcagcttc
      841 tgcttgtggc actcgtcgat caccgcgcgg tagacctcgg gctgcatctt ggggaacttg
      901 ccgaagttgt cgtcgaccca tagcttgacg atgtcgacgt ggtggccggc catctcgcgg
      961 acctgggcgc gcgcctcgtc ggcggtggcc gcgcggtaga cctggtcggg ctgcaccggc
     1021 tggggcggca cgctgccggg cgcgccgatg ccgcggccgg cggtgaagat gctggcgccc
     1081 gggacggtgc cctggcgctg ctgatcgcgc agctcgtaca ccagatcgcg attgagcccg
     1141 agcgtgacga acgacgtgac gccgtactgc tcgtactgcc gggcggcggc ctggaccgcc
     1201 tcgcgggtgt aggcatcggc gctgttggcg gcgccgatca ccagcccggc gtggctgtgc
     1261 gcgctgatca gcccgggcat gatcgtgcgg ccgtgcaggt cgacgacccg cgcgcccttg
     1321 ggccgggcca gcttgccggt gacgccgacg gcggtgatct tgtcgccctc gatcaccagc
     1381 gcggcgttgt cgcgcggcgg cttgccggtt ccgtcgatca gccgcgcgcc ctcgaggatc
     1441 accggcgccg ccgccgggcc ggcgtgggcc accccggcga gcgccgggac ccaggccggg
     1501 atcaacatca tcgcgagcat cgcgaacccg agacaccatc gtgagcagcg catcgtcgtc
     1561 ctcctggccc agcggaaatc gcccatctga ccctgggcgc ctggacgggc atacaatatc
     1621 ggaaagcgat gaccgtccca cccgaccagc gccagatccg gggcgcctgc gcgctcgatt
     1681 gcccggacac gtgcagctgg gtggtgaccg tcgagggcgg ccggccggtc gcgcttcgcg
     1741 gcgatcccaa ccacccctac acgcgcggcg cgctgtgcaa caaggtcgcg gattacctgg
     1801 cgtacacgcg ctcgcccgac cgcgtgctcc atccgatgcg gcgcgtcggc cccaagggca
     1861 gccaccagtt cacccggatc tcgtgggacg aggcgctcgc gcagatcgcc gaccggctcc
     1921 gcgacgtgat cgcgcgccat ggcgccgagg cgatctggcc gttcctgggc accggcaaca
     1981 tgggggtgct gcagggcgcc tacggcgccg ggcgccgggc gccggctgtg gaacgtgctc
     2041 ggcgcatcgc gccacatcca gacgatctgc atgatcgccg gcgggtacgg caccggctac
     2101 acgctgggcg acaaccgggt cgggatggat cccgagaccc tgcggctgtc ccggctgatc
     2161 gtgctgtggg gccacaacgc gctgagcacc aacgcccacc tgtggcgctc gatcctcgag
     2221 gcgaagagcc gcggcgcgac gcgggtcgcg caagctcgag ttcgtgtccg agcgcatggc
     2281 ccaggccggc ctcgatccgg tcgccggcta cacgccgtcc gtcgagaccg gccagcgcga
     2341 cacgccgctc gcccgcgact acccgctcgc gctggtctcg ccggccaacc actacttcct
     2401 gaactcgatc ttcgccaacg tcgcgcacca gcagcgccgg gcgggtcccg cgacgctgat
     2461 gatccacccc gacgacgccg cgccccgcgg catcgcggcc ggcgacgagg tccgggtcgg
     2521 caacgggatc cgccggtccg ggaccccgcc cggctccctg atgttcctcg gcctcgatgc
     2581 ggagatctcg agctgctcaa gagcctgacg acgcatcagc ccgagcgtcc gatcgcgccg
     2641 gccgcagtgg ctccctgtga cccgacgcgc agacgggtag agcctgagcg ccgtggtgtg
     2701 attgatgcac ctccggcgga catgccgcgc tcggttcgca aggtcctgcg tgtgacgggg
     2761 ttctcgctcg tcgcgcttct cgtgctcgcg caattcgtcc cctacgggcg ggcgcatacg
     2821 aatcgtcctg tccacgccga gccgagctgg gatggcccca cgacgcggga gctcgcgcgt
     2881 cgcgcgtgct ttgactgcca cagcaacgag acccgctggc cctggtactc ccacgtcgcg
     2941 ccgacgtcgt ggctggtcca gcacgatgtc gacgagggcc gcgagatcct gaacttctcg
     3001 gagtggaacc gcccctacga ggaagccggc gaggcagccg agacggtcct ggagcgtacg
     3061 atgcctccgc gggcctacct gctgttgcac cccgaggcaa aactcacgga cgtgcagcgg
     3121 cggcagctcg cggacgggct gaacgcgacc ctgggcacct cgcgcgaacg cgagtgacta
     3181 ctcgactttg gtgtacggct cgcggccggt aggctttgtt acggtgtctc ccgacaccgt
     3241 gccgacgtag tagtccacgg gcgggttgtg accgaggttg gcgagcgcgt tcgcgaacaa
     3301 cctggcgtgc tcggcctcgg tgttcatcgc gtaggcgaag ctcttcgccg cggccggcac
     3361 gtgctcgcgg cgcgcctccg cgatcatcgc cgggtacgtc ttctcctcct cgttgcgttc
     3421 ggccttgatc ggagcctcga ggttctcgcg cgtcgtcccg acgatcacgt tctcgatcac
     3481 cggcggcctg ggctcgagtc ccatggcgcg cagcaccgcc tcgtcgtcct tctcgtggat
     3541 cgactcggcg agcgcggtcg cgcgaaacag cttggcgacc tgcgggttgc cctccctgtc
     3601 ggcctgctcc gcgaacagct tgtagcgatg agacgcgttg gcttcgccct ggatcgccgc
     3661 gttcaggtta tcgatggtgc gctgcgggct cgctgcgggt tgcgcgaagc tcgtgaacgg
     3721 ccacagtacg agtgcagcca ctgccaggat catcttcagc atacaacctc cgctaccctg
     3781 ccaagcaccg cgcgtgccgc ggcggtgtgg atgacgcgtt ggccgcagga ggcttgctcg
     3841 ctggcggccg cgaccgagcc accgtgggct cgacgatgac cttgatcgag cagcgcgtcg
     3901 tgtcggcaag aaggtcgtga tggcggtcac cggtaccatc atgatgctgt tcctcatcgc
     3961 gcaccttgcc ggaacgtaac gttcgcacca cgagccgaag gagtctacat gaccaagccc
     4021 atgtcgatgt ccgacggaat cgagctcgaa ctcccggccg ggcggttgct cgtgatcgca
     4081 cccgggctcg cgcacgacgt ggaggccgtg gccgacagcg cgttcctgct cacgctcgcg
     4141 cgcggggcgc agggctgacg tcatgccggt ccgcgcgcga gagctttcgt tcgtgttgca
     4201 cccgatcgcg ccgttccgcc tcgatctcac cgcgtgggcg ctgcgacgcc gggccaacaa
     4261 cgagatcgat cgatgggacg ggcgtaccta cgcacggacg atcgccgtca acgaccgcgc
     4321 ggtcgatctc gaagtcacgc aggccggatc cgcgacccgg cccgcgctgc acgtcgtcgc
     4381 gcgcggagcg gcgggccacg cggtccgcgc gacggcaacg gccgtgctgt cgcgcgcgct
     4441 cggggtcgac atcgatcttg cagacttcta tcggatggcg cgcggagatg cgcggctcgc
     4501 gctgctcgtc gagcgatttc gcgggctcaa gccgccgcgc ttcccgaccg tgttcgaggc
     4561 ggtggtgaac ggcatcgcgt gccagcagct cagtctgacc gtgggcatca tcttgctggg
     4621 ccggctgtgc aacctgtgcg ggccgagctc gtccagcggc gcgcacgcgt ttccgcggcc
     4681 gaaggacatc gcggcgctga cgccggcgcg gctacgcgcg ctcggcttca atcgtaacaa
     4741 ggcgcgcggg ctgctcgagc tcgcgtgtgc ggtcgagggc ggcttcgatc tcgaagggct
     4801 cgccgacctc gacgacgagg tggtggtcga gcggctcgtc acgctgcgcg gggtcggccg
     4861 ctggaccgcc gagtatgcgc tgttacgtgg actcggccgg ctcgcgatgt tccccggcga
     4921 cgacgtcggc gcccgcacga acctcgcgcg ctggatgaag ctgcgcacgc cgctcgacta
     4981 cgatcgcgtg cgccggctcg cgcatcgctg gcagccctat ccgggcttcc tctacttcca
     5041 cctgctattg caatcgctcg acgagaccgg cacgctcacg ccggccgttc ccgccagccg
     5101 agcgtcatcc gtgctcgccc gatccggtcc gttcggcccc gatctcggaa agcgcgatcg
     5161 tcgcgtgcgc gaacgcgccg ccggcacgca tctcggcggc gacccagatc gcttccatca
     5221 gctcgttggc cgtggccccg gcacgtagcg ccgccttcgt gtggccgcga atgcagtagg
     5281 ggcattgtgt gacgtgcgcg accgcaactg cgatgatctg cttcgtcttc gcggacagcg
     5341 cgccgtctgc gaacacctgc ttcgagaacg cccggaacgc ggtatccgta tcgggtgcga
     5401 gctcgtgccg cgtgatggct cgtcgcgtgc acgcaactgg atcatggcgt ccctgacggt
     5461 aggatcagag cccgaggcca ggcgtgtcgc gctcggcatc acgcgccctt gctcgccgcc
     5521 cggg
//
LOCUS       DASVOR010000196         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_381846,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000196 DASVOR010000000
VERSION     DASVOR010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381846"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF516_04405"
     CDS             <1..300
                     /locus_tag="VF516_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106944.1"
                     /translation="RLARRAPPPGAGPSAEARARGHWIVRLVAEGDSGERLVYIASDR
                     ADPGYGSTSKMLGEAALCLARDPLSSPGGVLTPSVAMAQPLLDRLRRAGLSFAPA"
     gene            401..1027
                     /locus_tag="VF516_04410"
     CDS             401..1027
                     /locus_tag="VF516_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX8106945.1"
                     /translation="MFGRFLPRETSFFDFFEQHAALTIEGTKEFLSLVTTGTNLAAKC
                     RRISDIEHETDTITHRCVEALHKTFITPIDRDSIHRLITKMDDVMDFVEAAAERLDLY
                     ELTVMTADVRDLADVLHRSAQQVEVAVRGLRSLGDPQATLKLCIDINRLENEADAILR
                     RSVARLFKEEKDPILIIKWKEVYENLENATDRCEDVANIIEGVILEHA"
     gene            1034..>1990
                     /locus_tag="VF516_04415"
     CDS             1034..>1990
                     /locus_tag="VF516_04415"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX8106946.1"
                     /translation="MISVIVIVTIVAALIFDVINGFHDAANSIATVVSTRVLSPKIAV
                     VWAAVFNFIALFIFHQGVADTISKIITVDKGPSYVIVVVCGLTGAILWNLLTWLWGLP
                     SSSSHALIGGLSGAGIAFRGTSVLQWESLEKTLEWIVLAPVVGLVLGSLLMFAVFWLF
                     RGFRPQRVDRIFRVGQLLSAAAYSIGHGGNDAQKTIGVIWAVMIAGGMLNASAGSAPT
                     WVVIASYTAMAIGTAVGGWRIVKTMGMGLTHLKPVGGFCAEIAGAITLFTATAFKIPV
                     STTHTITGAIIGVGSVNTARGIRWSVATRIVYAWIFTIPAAGM"
BASE COUNT          295 a          689 c          679 g          327 t
ORIGIN      
        1 cggctcgccc gccgtgcgcc cccgcccggc gccgggccgt ccgccgaggc ccgcgcccgc
       61 ggccactgga tcgtccggct tgtcgccgag ggcgacagcg gtgagcgcct ggtgtacatc
      121 gcgtccgatc gtgccgatcc gggctacggc tcgacctcga agatgctggg tgaggcggcg
      181 ctatgcctgg cgcgagatcc gctgagctcg ccgggcggcg tgctcacgcc ctcggtcgcg
      241 atggcacagc cgctgctgga ccggctgcgc cgcgccggcc tgagctttgc cccggcctag
      301 gagccgcgtt catggcggga gcgccgccgg gagtggggaa aggcccctgc cgcgtgacgg
      361 cgcggtcgcg gccgtgttac acgctcgcgc aggagctcgc atgttcggtc ggttcttgcc
      421 ccgagagacc agcttcttcg acttcttcga gcagcacgcc gcgctgacca tcgagggcac
      481 gaaggagttc ctgtcgctgg tgaccaccgg caccaacctc gcggcaaagt gccggcggat
      541 ctcggacatc gagcacgaga ccgacacgat cacccaccgc tgcgtcgagg cgctgcacaa
      601 gacgttcatc acgccgatcg atcgcgactc gatccaccgg ctgatcacca agatggacga
      661 cgtgatggac ttcgtcgagg ccgccgccga gcggctcgat ctctacgagc tgaccgtgat
      721 gaccgccgac gtccgcgacc tcgccgacgt gctccaccgc tcggcccagc aggtcgaggt
      781 cgcggtccgc gggctgcgct cgctcggcga tccgcaggcc acgctcaagc tgtgcatcga
      841 catcaaccgg ctggagaacg aggccgacgc gatcctccgc cgctcggtcg cccggctgtt
      901 caaggaggag aaggacccga tcctcatcat caagtggaag gaggtctacg agaacctgga
      961 gaacgcgacc gaccgctgcg aggacgtcgc caacatcatc gagggcgtga tcctggagca
     1021 cgcgtagccg cgcatgatct ccgtcatcgt catcgtcacg atcgtcgcgg cgctgatctt
     1081 cgacgtgatc aacgggttcc acgacgccgc gaactcgatc gcgaccgtgg tgtcgacccg
     1141 cgtgctgtcg cccaagatcg ccgtggtgtg ggcggcggtg ttcaacttca tcgccctgtt
     1201 catcttccac cagggcgtcg ccgacacgat ctcgaagatc atcacggtcg acaagggccc
     1261 gtcgtacgtc atcgtcgtgg tgtgcgggct gaccggcgcg atcctgtgga acctgttgac
     1321 ctggctgtgg ggcctgccgt cgtcgagctc gcacgcgctg atcggcgggc tgtccggcgc
     1381 cggcatcgcg ttccgcggga cctccgtcct gcagtgggag agcctcgaaa agacgctgga
     1441 atggatcgtg ctggcgcctg tggtcggcct ggtgctcggc agcctgctga tgttcgcggt
     1501 gttctggctg ttccgcgggt tccggcccca gcgggtcgac cggatcttcc gggtcggcca
     1561 gctcctgtcg gccgccgcgt actcgatcgg ccacggcggc aacgacgcgc agaagacgat
     1621 cggcgtgatc tgggcggtga tgatcgccgg cggcatgctg aacgccagcg ccggcagcgc
     1681 gccgacctgg gtcgtgatcg catcgtacac cgcgatggcg atcggcaccg cggtcggcgg
     1741 ctggcggatc gtcaagacca tggggatggg gctcacgcac ctcaagccgg tcggcgggtt
     1801 ctgcgccgag atcgccggcg cgatcacgct gttcaccgcg accgcgttca agatcccggt
     1861 gtcgaccacc cacacgatca ccggcgcgat catcggggtc ggctcggtca acaccgcgcg
     1921 cggcatccgc tggtcggtcg cgacccgcat cgtgtacgcc tggatcttca cgatcccggc
     1981 cgccggcatg
//
LOCUS       DASVOR010000197         3346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_60406,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000197 DASVOR010000000
VERSION     DASVOR010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60406"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF516_04420"
     CDS             complement(<1..225)
                     /locus_tag="VF516_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106947.1"
                     /translation="MPWLRHRLRDADVWAKVDANAALIKDRDGRVEIVYKPAGGAKVY
                     RAGARNLTPAPDSEPVEFEAGEPAEPRSRDA"
     gene            294..1277
                     /locus_tag="VF516_04425"
     CDS             294..1277
                     /locus_tag="VF516_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106948.1"
                     /translation="MVRIRQRWSIVLLGLVLGVGFVACKKDSTGGAGDKSSEASVGAP
                     GVGDLALLPADSEVVFGMNLGQMQQSALWKQFVEPKLANAKSQRMMSEFKAKCGADPM
                     KMVSSFTFGAKDFSGDKPSVVAVAHGLDKAKILDCIEKSKADIAKDGGEVTRDGDVVL
                     FKGDRGEPAALQFINDSTAVLVAGPSGNAAGVKAVVGGGSSLKSSAPFLDMYKKVKTG
                     DSIWGLASGKVMESVPLDLKATAAYGSINITDGLSVDMRVRFATPDAATQAATMANAQ
                     TKQATQYVDKAEATADGNELHLAIVISGQKLQQLAPMLSMFAGGIPGMGGN"
     gene            1336..2367
                     /locus_tag="VF516_04430"
     CDS             1336..2367
                     /locus_tag="VF516_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106949.1"
                     /translation="MVVQRSALYLILALCAAVPGCGKKDVPSGPPPELTGLAVVPASV
                     EVVVGADLVKLTDAPVIDRALEQLLMRDATLSDRWQRLKDECKIDLGKQIKRVMLALG
                     PNRPGPAPGTGPVLMVAVGSLQETELQQCVTKLIGAGGGSVTGKSVAGRTVYLAKDGN
                     RTMYFAYTRPDTVVLSADEAYLTEALGPGKKAPDNPDLTAWRKLVNQSAPLWAVGRTD
                     PRIRDGLVQLTERKISEGPVAFALSADLADGARLQLSAVMNQPDQAKALESYVKGELA
                     LLTAAAQLRSLGSVVGKIAVASDGNVVQFHAALTVDDLNRLLSVLDGDSTPPQNRAPP
                     QPSPGSGAQ"
     gene            2407..2823
                     /locus_tag="VF516_04435"
     CDS             2407..2823
                     /locus_tag="VF516_04435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106950.1"
                     /translation="MKKPAAKAKPAAKKAPPKAKAKAPAAPAKILPRHPRARVAEAHG
                     SKAALAKAIAPLLARTDEDTDALEVRLKKASNAQLLRLQRATQTLKDKYGNREKLISA
                     IGQAQRKAKDKDYLAKLETYSLPYLLDLAASASARA"
     gene            2951..>3346
                     /locus_tag="VF516_04440"
     CDS             2951..>3346
                     /locus_tag="VF516_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106951.1"
                     /translation="MRAVFSGVLRSVFSVTAAPDPDTERRSRLLLLMAAALLVLLLLS
                     FATVDLVRGNAQTWVVVSPIGMFAMLVTIVLARRGRANAGGLVLSVFVCAAIVAIAWT
                     QGPGCVRMSVAVLSVVAVGSVLNTRLTAVV"
BASE COUNT          540 a         1174 c         1158 g          474 t
ORIGIN      
        1 cgcgtcgcgc gaacgcggct cggccggctc gccggcctcg aactccacgg gctcgctgtc
       61 cggcgccggt gtgaggttgc gcgcacccgc gcggtagacc ttggcaccgc ccgcgggctt
      121 gtacacgatc tcgacgcgcc cgtcgcgatc cttgatcagc gcggcgttgg cgtcgacctt
      181 ggcccacacg tcggcgtcgc gcaggcgatg gcgtaaccag ggcatggcgg accctatcac
      241 aggatgttat aaggcccggc ggcgtccaga gcggatgcta caggagacta ccaatggttc
      301 gcattcgcca gcgctggagc atcgtcctgc tcggtctcgt gctcggggtg gggttcgttg
      361 cgtgcaagaa ggacagcacg ggaggtgcgg gagacaagtc gagcgaggcg agcgtcgggg
      421 cgccgggcgt cggcgacctc gcgctgttgc cggccgactc cgaggtcgtg ttcggcatga
      481 acctgggaca gatgcagcag agcgcgctgt ggaagcagtt cgtcgagccc aagctcgcga
      541 acgccaagag ccagcgcatg atgagcgagt tcaaggccaa gtgcggcgcg gacccgatga
      601 agatggtctc gtcattcacg ttcggcgcca aggacttctc cggcgacaag ccgtcggtgg
      661 tcgccgtcgc ccacggcctg gacaaggcga agatcctcga ctgcatcgag aagagcaagg
      721 ccgacatcgc caaggacggc ggcgaggtca cccgcgacgg cgacgtcgtg ctgttcaagg
      781 gcgatcgcgg tgagccggcc gcgctgcagt tcatcaacga ctcgaccgcg gtcctcgtcg
      841 cgggccccag cggcaacgcc gccggcgtca aggcggtggt cggcggcggc tcgtcgctga
      901 agagctcggc gccgttcctc gacatgtaca agaaggtgaa gaccggcgac tcgatctggg
      961 ggctcgccag cggcaaggtg atggagagcg tcccgctcga tctcaaggcc accgccgcgt
     1021 acggctcgat caacatcacc gacggtctgt cggtcgacat gagggtccgg ttcgcgacgc
     1081 cggatgccgc gacccaggcc gcgaccatgg ccaacgcgca gaccaagcag gccacgcagt
     1141 acgtggacaa ggccgaggcc acggccgatg gcaacgagct ccacctcgcg atcgtgatct
     1201 cgggccagaa gctccagcag ctcgccccga tgctcagcat gttcgcgggc ggcatccccg
     1261 gcatgggcgg gaactagcgg tcggagcgag cggagccgcg agtcccggag cccacggagt
     1321 cacggagtcc aagcgatggt ggtccagcgc agcgcgctct acctgatcct cgcgctatgc
     1381 gcggcggtcc ccggttgcgg caagaaggac gtgccgagcg gaccgccccc ggagctcacc
     1441 gggctcgcgg tggtccccgc gtcggtcgag gtcgtggtcg gcgccgacct cgtgaagctg
     1501 accgacgccc cggtgatcga tcgcgccctc gagcagctgc tgatgcgcga cgccacgctg
     1561 tccgatcgct ggcagcggct caaggacgag tgcaagatcg atctcggcaa gcagatcaag
     1621 cgggtgatgc tcgcgctcgg cccgaaccgt cctggccccg cacccgggac cggtccggtc
     1681 ctcatggtcg ccgtcggctc gctgcaggag accgagctcc agcagtgcgt gaccaagctg
     1741 atcggcgccg gcggcggcag cgtgaccggc aagtcggtgg ccggccggac cgtgtacctc
     1801 gccaaggacg gcaaccgcac gatgtacttc gcgtacaccc ggcccgacac cgtcgtgctc
     1861 agcgccgacg aggcgtacct caccgaggcg ctcggccccg gcaagaaggc gcccgacaac
     1921 cccgatctca ccgcatggcg caagctggtc aaccagagcg cgccgctgtg ggcggtcggc
     1981 cgcaccgacc cgcggatccg cgacggactg gtccagctca ccgagcgcaa gatcagcgag
     2041 gggccggtcg cgttcgccct cagcgcggat ctggccgacg gcgccaggct ccagctcagc
     2101 gccgtgatga accagcctga ccaggccaag gccctggaat cctacgtgaa aggcgagctc
     2161 gcgttgctga ccgcggcggc gcagctccgg tcgctcggat cggtggtcgg caagatcgcc
     2221 gtggcttccg acggcaacgt cgtgcagttc cacgcggcgc tcaccgtcga cgacctgaat
     2281 cgcctgctat cggtgcttga cggcgacagc acgccccccc aaaatcgcgc gcccccgcag
     2341 ccgagtcccg gctccggcgc gcagtagcga agcgagacgc ccatgcccag caacaagacc
     2401 ccgtccgtga agaagcccgc cgccaaggcg aagccggctg ccaagaaggc cccgcccaag
     2461 gccaaggcca aggcgcccgc tgcgccggcc aagatcctcc cgcgccatcc cagggcccgc
     2521 gtcgccgagg cgcacggctc caaggccgcg ctcgccaagg cgatcgcccc gctgctcgcg
     2581 cgcaccgacg aggacaccga tgcgctcgag gtccggctca agaaggcctc gaacgcccag
     2641 ctgctccgcc tgcagcgcgc cacgcagacc ctcaaggaca agtacggcaa ccgcgagaag
     2701 ctgatctccg cgatcggcca ggcccagcgc aaggccaagg acaaggacta cctggccaag
     2761 ctcgagacct actcgctgcc gtacctgctc gatctcgccg cctcggcgtc ggcccgcgcc
     2821 tgaccgagtc ggttccggcg gcccgcggcg tgcgccgcct caccgccgga cagtgagaac
     2881 tgcggccacg ctggataccc cgcagctctc gtgctcgtga gacgccgcgt cgatcgagta
     2941 tcatctcggt gtgcgtgcgg tcttctcggg ggtgctccga tcggtgttct ccgtcacggc
     3001 tgcgcccgat ccggataccg agcgccggtc gcgactgctg ctcctgatgg cggcggccct
     3061 cttggtcctg ctgctgctgt cgttcgcgac ggtcgacctc gttcgcggca acgcgcagac
     3121 ctgggtcgtg gtctcgccga tcggcatgtt cgccatgctg gtcacgatcg tgctcgcgcg
     3181 gcgcgggcgc gccaacgcgg gcggtctggt gctgagcgtg ttcgtctgcg cggcgatcgt
     3241 ggcgatcgca tggacccagg ggcccggctg cgtccggatg agcgtggcgg tgctcagcgt
     3301 ggtggcggtg ggctcggtgc tcaacacgcg attgacggcc gtggtc
//
LOCUS       DASVOR010000198        14017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_381850,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000198 DASVOR010000000
VERSION     DASVOR010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14017
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381850"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            366..1079
                     /locus_tag="VF516_04445"
     CDS             366..1079
                     /locus_tag="VF516_04445"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8106952.1"
                     /translation="MRIAQLSDIHVGSLTPASHIRAAIAAANAAAPDLIVLTGDYVCW
                     RRHEVALAAEQLGGLTAPRVLAVLGNHDYYAWADGVASALERNGYEVLRNRATVARVR
                     GAPLAVVGVDDPVTRHDDLDASFAGAPVGTTRIVLCHLADRGPELARRGADLVLSGHT
                     HGGQIYVKGITDRLMKRIGLHYRRGLYDIGSHARRSTLYVTPGVGFSGVTRRHGEGTH
                     AEVAVLTLRMAPPRVARAA"
     gene            complement(1126..1989)
                     /locus_tag="VF516_04450"
     CDS             complement(1126..1989)
                     /locus_tag="VF516_04450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8106953.1"
                     /translation="MTDSAARRRHGLALAATSASLFSLNTVCARTAFDAGANAITGNA
                     ARSAFAAAVFGLLLLLRRGRTRLSGRQRLAAAALAVPVALSSFGYLGAVEYIPVSTAV
                     LVVYTYPIWVGLIARLTRGEPLGGARVVAIFLAFAGVAIALDVHASAMPAARGLALAA
                     LAASGMAAMVLGSSRLVRDTDPGAINFYLSSAATGIFAVLLLVGDPRLPHSALGHLGF
                     AGLLVTFPLAQLASLAAVDRAGPVVTAVAMNFEPLITIGLAVILLGEHVSAIQLGGAA
                     LVVAAIAIMRR"
     gene            complement(1982..3190)
                     /locus_tag="VF516_04455"
     CDS             complement(1982..3190)
                     /locus_tag="VF516_04455"
                     /inference="COORDINATES: protein motif:HMM:NF037663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="styrene monooxygenase/indole monooxygenase
                     family protein"
                     /protein_id="HEX8106954.1"
                     /translation="MALNAASVGIVGAGIGGLHLGLYLRQHGVAATLYTDRSADAVRT
                     GRLLNTASLGGDTRDRDRALGVAYWDTPDAQVSCIHFDLEQPALRFRGDFARPNLLVD
                     MRHYLPRLLEAFEARGGRVVVQEVDPAQLTEFARDHACLVVASGRSGLSELFPRIPEH
                     SPFREPQRRILAVLCTGIRPSAPPGLTIQVVPGRGELLDFPFLREDGRVGCINVQAIP
                     GGPFEPMARMRHDDDPAAFRAALLALFLAHAPLTRPRIDPGEFDVLGPSHVLQGGITP
                     TVRRGHAALGDGRFAIALGDAHITHDPIGAQGANAASRAAWILGELIVARLAAGRSFD
                     AEFHAQAEQTLWQATRAATEWSNVLLRPPSQRFVDLLVAASRDQTIANAYVDNFTDHP
                     ERQLDALRDD"
     gene            complement(3181..4353)
                     /locus_tag="VF516_04460"
     CDS             complement(3181..4353)
                     /locus_tag="VF516_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX8106955.1"
                     /translation="MLIDGSVKTIRRFRISAARRLYHPGWTAEQNARVFGNRTTELGY
                     GANFTMFVAVESRIDPTTGMTMHTDDIRAVVDPVLAKFDHRHMTIETDHFTRMPATSE
                     AIASVLFELIRPGLPEHVQLANVVVWESRSRWADRSPSAIRSGALHDVFWRTDETALP
                     TAHSTLTVVRAGDALGVEAQPIDDACEAFAREAKPRAGDARAAFSLALAALKERLAEV
                     CSAGSSQIDRVALSDAFLGELSLDAADVVRLSRAYDFEATHRMYNAQLSEAENYQLFG
                     KGTNPFGHGHSFHVRVSARVGGREDAPALARGMDDAARTVIGQLDRRRLELEVPFFQD
                     RVATSENVALWIWSELRERLGERLDGLTVWGTENNLYEIGNEMPRLSGDDRAQTWH"
     gene            4734..5684
                     /locus_tag="VF516_04465"
     CDS             4734..5684
                     /locus_tag="VF516_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HYR domain-containing protein"
                     /protein_id="HEX8106956.1"
                     /translation="MLFNISDRAKLGILEVQWQQSNQPFGIAFTPNGAFAYVANFVGS
                     SVSVIDTATTTVVASISDAQARTPAGVAITPDGAFAYTVNFNSNTVSAIDTATNTIVA
                     IIPGLVGPEGIAITPNGAPKASCHDVTVAAGPSCTATASVENGSVDPNHDPLTLSQAP
                     AAPYSLGATGVTLTATDNFGASGHCTATVTVVDNTPPAITCPADITTKGNIPNSPFAS
                     VDPGTPTATDDCSGLTVVGTRGDGQPLNAPYPFGTTTITQTATDAGGNQSSCQQTITV
                     VPNAPISKDQCKDGGWQSFTNPTFNNQGDCVSFVECLPPQ"
     gene            5721..8087
                     /locus_tag="VF516_04470"
     CDS             5721..8087
                     /locus_tag="VF516_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8106957.1"
                     /translation="MTDRATSLRAARAGSCGSLHEVSAATHSSHWGAFTVRTRDGEID
                     AIEPLHDPDPSPLLGNLRGSIRHRSRIARPVARRGWLERGPGPTGDRGRNEFVAIPWD
                     EALERAADELRRVIAAHGNAAIFGGSYGWASAGLFHQSQSQLRRFLNQIGGFTASRNS
                     YSIGASLVLLPHVVGDARAVFYKATPWTMIAKHTELLVAFGGIPLKNTFVAPGGMARH
                     GLRDHLADARRRGMQIAHLSPLRDDVLAEAEARWYPLAPLSDVAVMLALCHVLVTEGL
                     HDEAFLASCCHGADRFIAYVRGDSDGVAKTPEWAERISGLPAEDLRALARRMAASRTL
                     VTVSFSLQRAEHGEQPVWAGLALAALLGQIGLPGGGFGHGYGSMGELGSASAVIPLPF
                     VPEGQNPVKSFIPVARIADLLLHPGEPFDYDGARHVYPDVRLVYWAGGNPFHHHQDLN
                     RLRRALGRPDTVIVHEPYWTPMARHADLVFPATTSFERNDIGSGRADARIVAMRRAVE
                     PHAEARNDHDILAGLAERLGVARAFTEERDERAWLSSTWAKLRASLEAAGIAAPDFET
                     FWRDGSFAVPDTEAERVLFDAFRADPAGNPLKTPSGRIELYSETIAGFGYDDCPGHPA
                     WLDKSEALGSPRARRYPLVLVANNPATRLHSQLDHGEYSQSAKVNGREPVRIHPEDAA
                     ARGIANGDVVRLSNDRGSCLAGAVVTEDVRRGVVQLSTGAWFDPDDPAAEIATCVHGN
                     PNVLTRDAGTSRLSQGCSGQHALVEVERWNGPLPPVRAFEPPAVAERS"
     gene            complement(8099..9106)
                     /locus_tag="VF516_04475"
     CDS             complement(8099..9106)
                     /locus_tag="VF516_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106958.1"
                     /translation="MSARDRHNADLFYAELMGHAMPRGRASEDDLTEGTAAFPTLKQA
                     WDTDKRRILFSFKPISTTFTDASGIFNARFWVSSDAVKWKIPASERDLLAEWNRFKEA
                     DPIDQDGDMLCRLPCTARQNQAFANALKLHPDPRTPGAEDGLIDRTAPIRSDMAPEPC
                     LLLTPYLYARRYQQVSCKIDPVGRDPGAPIVGEAAKHNQYLNQKTTAFMRARNDSYRV
                     PSIGDPGKIWAITNQMYVSRARNGQPSAINYGWHGPNATLASVIAGLKCLQSVGTTHD
                     DRHVDYSQVCVLVAGWCEVTEPGQSAPAWVKTESVYTSARLCKLVTEDGKPLARVRYE
                     T"
     gene            complement(9696..11504)
                     /locus_tag="VF516_04480"
     CDS             complement(9696..11504)
                     /locus_tag="VF516_04480"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8106959.1"
                     /translation="MIDDDAPAGASQDPTLRASETPSDKASDRVDRVLDRRDLFVGGA
                     TLVAGAALTACGTREEPRPAQPPPPKPSPNAKAMAELEAKAKANASVTESLPAPELAE
                     LTFADLQARMAAGTDTAKSLVAKYRQRIEAIDQKGPTLRSVLELNPDADSIADQLDAE
                     RKAGKLRGPLHGLPILVKDNIDTGDKMTTTAGSLALEGSHAEKDAFVVARLRAAGAII
                     LGKTNLSEWANFRGKASLSGWSGRGGQCRNPYALDRTPSGSSSGSGVAAAASLCAAAI
                     GSETDGSIVSPSSSNGLVGVKPTIGLVSRAGVVPLSASQDTLGPMARTVTDAAQLLTV
                     IAGPDPDDPVTTAPDKARPAAPEDYTKYLDPKALAGARLGVPQKGFFGLSRGVDALMR
                     GALGDLKDAGAVLVEQAELIVPPELDAAETEVLLFELKAYLDKYLAARKADAHVHSLA
                     EAIAFNFQNRGAELALFGQEHFEEADKKPGLTDKAYRDARAKCLQITRTLLLDKVMAE
                     HKLDAFVAATSGPAWLIDPVNGDAGSGVSCSTLPAVSGYPHVTVPAGQVRGLPVGLSF
                     FGKPYTEGKLLGYAFAYEQKTKHRVPPRFLPSVEMA"
     gene            11652..12284
                     /locus_tag="VF516_04485"
     CDS             11652..12284
                     /locus_tag="VF516_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8106960.1"
                     /translation="MITLHSAPATAGMVVHWLLLELEVPHTLRMLDLEAREHKQPAYL
                     ALNPAGVVPTLVIDGQPICEAAAIALHLTDTYPARGFAPATGTLARAAYYRWMFFLAN
                     TIQPAFRAWFNPTELAGEDHVAVVKAQAEARIADGWNILDAHLAAHGPYVLGERVTTP
                     DFMLTMLMRWSRHFPRTALEWPALARLATAMKARPTFKELYAREGLTDWA"
     gene            complement(12310..12723)
                     /locus_tag="VF516_04490"
     CDS             complement(12310..12723)
                     /locus_tag="VF516_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106961.1"
                     /translation="MQNLRILACVAAALGSIAIAGCGGDGNDHGQLNVHNESDFAVVE
                     IRVTSVGSTTWGPNLISGDILAPGESLAIDVSCDQYDALLVDDSGAQCTVHDIDLCFN
                     TADWIIRNDTCPVFASARAAREAAQAAGSGSSAAH"
     gene            complement(13090..13488)
                     /locus_tag="VF516_04495"
     CDS             complement(13090..13488)
                     /locus_tag="VF516_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106962.1"
                     /translation="MVALGSTAIVGCGSDNDCDNDNNCVCDSNNNCVPAPPPPPHGAL
                     VVHNQCSVGIKEVHVAAVDTTSWGVNLISGTLTPGASLTVTVACGQYDVLLVDQAGDQ
                     SILHHVALCSSNADWIIGSDTCAAFTHKDP"
BASE COUNT         2064 a         4865 c         5013 g         2075 t
ORIGIN      
        1 gctgaggtcg gccttgcaat gcttgcaccg gccggcgatc ggatgaatca ggccgccgca
       61 cagcgggcag gggatgcggg cgtcacgggc ttcgcgtggg ccagaggctt cgaatgagcc
      121 agaggcgtcg gatgggccag atcgagccgc ggcagccatg cctcctcatg ctgccaggcc
      181 aacgcaggcg atgcaaagcc aaccaggcgc cgccacggtc gtgcgcgcca tcctggcaat
      241 ccaccgcgcg gcgttgccaa gcgaccgaga cgtcctacag tgccgcggat atggtggatc
      301 tcgttccacc tcgtgtttcg gaacacgagg tgcacgtcga gcggctggac ccgcgccatg
      361 atggcgtgcg gatcgcacag ctgtccgaca tccatgtcgg cagcctcact cccgcgagcc
      421 atatccgggc tgcgatcgcg gcggccaacg ccgcggcccc cgatctcatc gtgctgaccg
      481 gcgactacgt gtgctggcgc cgccacgagg tcgcgctcgc ggccgagcag ctcggcgggc
      541 tgaccgcgcc gcgcgtgctc gcggtgctcg gcaatcacga ctactacgca tgggccgacg
      601 gcgtggcgtc cgcgctggag cgcaacggct acgaggtcct gcgcaaccgc gccacggtcg
      661 cccgcgtgcg cggcgcgccg ctcgcggtgg tcggcgtcga cgatccggtc acccggcacg
      721 acgatctcga cgcgtcgttc gccggcgcgc cggttggcac cacccggatc gtcctgtgtc
      781 acctcgccga tcgcggcccc gagctcgccc ggcgcggcgc cgatctcgtg ctgtcgggtc
      841 acacccacgg cggccagatc tacgtcaagg gcatcaccga ccggctgatg aagcggatcg
      901 ggctgcacta ccggcgcggc ctctacgaca tcggctcgca cgcgcggcgc agcacgctgt
      961 acgtcacccc gggcgtcggg ttctccggcg tcacccgccg ccacggcgag ggcacccacg
     1021 ccgaggtcgc cgtgctgacc ctgcgcatgg ccccgccgcg cgtcgcgcgc gccgcgtagc
     1081 gccgggctcg cagccagaac gcctgcgggc cgcggttccc cctacttagc ggcgcatgat
     1141 cgcgatcgcg gccacgacca gcgcggcgcc gccgagctgg atcgcggaga cgtgctcgcc
     1201 gagcaggatc acggcaagcc cgatcgtgat cagcggctcg aagttcatcg ccaccgcggt
     1261 caccaccggc ccggctcggt cgaccgcggc gagcgaggcg agctgggcga gcgggaacgt
     1321 gacgagcagc cctgcaaagc ccaggtggcc gagcgcgctg tgcggcagac ggggatcgcc
     1381 gacgagcagc agcaccgcga agatgccggt cgccgccgac gacaggtaga agttgatcgc
     1441 gccggggtcg gtgtcgcgaa cgagcctgct gctgcccagc accatcgccg ccatcccgct
     1501 cgccgccagc gcggcgagcg cgagcccgcg cgcggcgggc atcgcgctgg cgtgaacgtc
     1561 gagcgcgatc gcgacgcccg cgaacgcgag gaagatcgcg acgacgcgcg ctccaccgag
     1621 cggctcgccg cgggtcagcc gggcgatcag gccgacccag atcgggtacg tgtagacgac
     1681 caggaccgcg gtgctgaccg ggatgtactc gaccgcgccg aggtagccga acgacgacag
     1741 cgcgacgggc acggcgagtg ccgccgcggc caggcgctgg cgcccggaca gcctggtcct
     1801 gccgcggcgc aggagcagca gcagcccgaa caccgcggcg gcgaacgccg accgcgcggc
     1861 gttgccggtg atcgcgttgg cgccggcgtc gaacgcggtc ctcgcacaca ccgtgttgag
     1921 gctgaacagc gatgcgctgg tcgccgcgag ggcgagaccg tggcggcggc gtgcggcgct
     1981 gtcagtcatc gcgcagcgcg tcgagctggc gctcgggatg atcggtgaaa ttgtcgacgt
     2041 aggcgttcgc gatcgtctgg tcgcgcgacg ctgcgaccag cagatcgacg aagcgctgcg
     2101 acggcggccg gagcagcacg ttggaccact cggtggcggc gcgcgtcgcc tgccacaggg
     2161 tctgctcggc ctgcgcgtga aactcggcgt cgaacgagcg cccagccgcg aggcgcgcga
     2221 cgatcagctc gcccaggatc cacgccgcgc gcgacgcggc gttggcgcct tgcgcgccga
     2281 tcgggtcgtg ggtgatgtgg gcatcgccga gcgcgatcgc gaaccggccg tcgccgagcg
     2341 cggcatgacc tcggcgcacc gtcggcgtga tcccaccttg cagcacatgg gaaggcccga
     2401 gcacgtcgaa ctcaccggga tcgatgcgcg gccgcgtgag cggtgcgtgg gcgaggaaca
     2461 gcgccaggag cgcggcgcga aacgcggccg gatcgtcgtc gtggcgcatg cgagccatcg
     2521 gttcgaacgg acctccgggg atcgcctgca cgttgatgca gccaacgcgt ccgtcctcgc
     2581 gaaggaacgg aaagtccagc agctctccgc ggccgggcac gacctggatg gtcaagccgg
     2641 gcggcgcgga cggccggatg cccgtgcaca gcaccgccag gatccggcgc tgcggctcgc
     2701 gaaacggcga atgctcgggg atccgcggaa acagctcgga cagccccgag cgacccgagg
     2761 cgacgaccag gcacgcgtga tcgcgagcga actcggtcag ctgcgccgga tcgacctcct
     2821 ggaccaccac gcgcccgccg cgcgcctcga acgcttcgag cagccgcggc aggtagtggc
     2881 gcatgtcgac caggaggttc ggcctggcga agtcgccgcg aaaccgcaat gcgggctgct
     2941 cgagatcgaa gtgaatgcag gacacctggg cgtccggggt atcccagtac gcgacgccca
     3001 gggcgcgatc gcggtcccgc gtgtcgccgc cgagcgatgc ggtgttgagc aggcggcccg
     3061 tgcgaaccgc atcggcggag cgatccgtgt acagggtcgc cgccacgccg tgctggcgga
     3121 gatacagacc gaggtgcagc ccgccgatgc cggcgccgac gatcccgacg ctcgccgcgt
     3181 tcagtgccac gtctgcgcgc gatcgtcgcc ggagagccgg ggcatctcgt tgccgatctc
     3241 gtagaggttg ttctccgtgc cccacacggt cagcccgtcg agccgctcgc ccaggcgctc
     3301 gcgcagctcc gaccagatcc acagcgcgac gttctccgac gtcgccacgc ggtcctggaa
     3361 gaatggcacc tcgagctcga ggcgccggcg atccagctgg ccgatgacgg tgcgcgcagc
     3421 gtcgtccatg cctcgcgcca gcgccggtgc atcctcccgg ccgccgaccc gcgccgagac
     3481 ccggacgtga aagctgtggc cgtgcccgaa cggattcgtg cccttgccga acagctggta
     3541 gttctccgct tccgagagct gcgcgttgta catgcgatgc gtcgcttcga agtcgtaggc
     3601 tcggctgagc ctgacgacgt cggcggcgtc gagcgagagc tcgccgagga atgcatcgga
     3661 cagcgcgacg cggtcgatct ggctcgagcc cgcgctgcag acctccgcga gccgctcctt
     3721 gagcgcggcg agcgcgaggc tgaatgcagc gcgcgcatcg ccggcgcgcg gcttcgcttc
     3781 gcgggcgaat gcctcgcacg cgtcgtcgat cggctgcgcc tcgacgccga gcgcgtctcc
     3841 cgcgcgcacc acggtcagcg tcgaatgagc cgtgggaagc gcagtctcgt cggtgcgcca
     3901 gaacacgtcg tgcagcgcgc ccgagcggat cgcgctcggc gatcgatcgg cccaccgcga
     3961 tcgcgactcc cacaccacca cgttcgccag ctgcacgtgc tccggcaggc ccggccggat
     4021 cagctcgaac agcacggacg cgatggcctc ggaggtcgcc ggcatgcgcg tgaagtggtc
     4081 ggtctcgatc gtcatgtgcc ggtgatcgaa cttcgcgagc accgggtcga cgaccgctcg
     4141 gatgtcgtcg gtgtgcatgg tcatgccggt ggtgggatcg atgcgagact ccaccgcgac
     4201 gaacatcgtg aagttcgccc cgtacccgag ctcggtggtg cggttcccga agaccctggc
     4261 attctgttcg gccgtccaac ccgggtggta caagcgacga gcagcgctga tgcgaaagcg
     4321 ccggatggtc ttgaccgagc catcgatcaa catggcgggc ggagccaact atctaccact
     4381 gccgctgcga aagcagccat gagcgccgag gaaaacgagc gccgtttcgc cggtggcgga
     4441 cggaggccgg ccccttgccc attctaacaa cttgataccc ctggagaatc tgctctcccc
     4501 agggaacgag agtttgggag cacttttggg gagcacttcg gggaccagct acctcggcag
     4561 cgtcgccgga tgcctgctcg gaccccatca cctccacccc agggcccgat cgacagcctc
     4621 ttgctcaccc agtggcatcc cgaccgtccg ctgccaggta gcgataccca tcgctcaccg
     4681 agatcgatcg gcgaagcgcc ttgatgcagt tctgccgcga tagatgattc tccttgctat
     4741 tcaatatttc tgatcgagca aagctcggca tcctcgaagt gcagtggcag caatcgaacc
     4801 agccgtttgg cattgccttc acccccaacg gggccttcgc gtacgtggcc aacttcgtcg
     4861 gcagctcggt ctcggtgatc gacacggcga ccaccaccgt cgtcgcctcc atcagcgacg
     4921 cacaggcccg cacccctgcc ggcgtggcca tcactccgga cggagccttc gcgtacactg
     4981 tcaacttcaa cagcaacacc gtctcggcga tcgatacagc aaccaacacc atcgtcgcta
     5041 tcatccccgg gctggtcggt ccggaaggca tcgcgatcac gccgaacggc gcgccgaaag
     5101 cctcgtgcca tgacgtgacg gtggccgccg gtccgtcctg caccgccacc gcgtccgtcg
     5161 aaaacgggtc cgtcgatccc aaccatgatc cgctgacgct cagccaggct ccggcggcac
     5221 cctactcgct cggcgccacc ggcgtcacgc tgaccgcaac cgacaacttc ggcgcgtccg
     5281 gccattgcac ggcgacggtg accgtggtcg acaacacgcc gccggcgatc acgtgtcccg
     5341 ccgatatcac gacgaagggc aacatcccga actctccgtt cgccagcgtg gatccgggga
     5401 cgcccacggc gaccgacgac tgctcgggcc tgacggtggt cgggacccgc ggtgatgggc
     5461 agccgctcaa cgccccctat ccgttcggga cgaccaccat cacccagacg gcgaccgatg
     5521 cgggagggaa tcagtcatct tgccagcaga ccatcacggt cgtccccaac gcccccatca
     5581 gcaaggatca gtgcaaggat ggtggctggc agagcttcac caatccgacc ttcaacaatc
     5641 agggcgattg tgtgtccttc gtggagtgct tgcctccaca atgaggttgc ggggcttcgc
     5701 gatcggcgat cctggggtcc gtgaccgatc gcgctacttc cctgcgtgcc gcccgcgcgg
     5761 gatcgtgtgg tagtctccac gaggtgtcgg ccgcgactca cagctcgcac tggggggcat
     5821 tcacggtgcg gacgcgcgac ggcgagatcg acgcgatcga gccgctgcac gatcccgacc
     5881 cgtcgccgct gctcggcaac ctgcgcggga gcatccgcca tcgctcgcgg atcgcgcggc
     5941 ccgtcgcgcg gcgcggctgg ctcgagcgcg gaccgggacc gacgggtgac cgcgggcgga
     6001 acgagttcgt cgcgatcccg tgggacgagg cgctcgagcg cgcggccgac gagctccgcc
     6061 gcgtgatcgc ggcgcacggc aacgcggcga tcttcggcgg gtcctacggg tgggcgagcg
     6121 cgggcctgtt ccatcagagc cagagccagc tccgtcggtt cctcaaccag atcggcggct
     6181 tcacggcctc gcgcaacagc tacagcatcg gcgcttcgct cgtcctgctg ccgcacgtcg
     6241 tcggggacgc ccgagcggtg ttctacaagg cgacgccgtg gacgatgatc gcgaagcaca
     6301 ccgagctcct ggtcgcgttc ggcgggatcc cgctcaagaa cacgttcgtg gcgccgggcg
     6361 ggatggcgcg ccacgggctg cgcgaccacc tcgccgacgc gcggcgccgc ggcatgcaga
     6421 tcgcgcacct gtcgccgctg cgcgacgacg tgctggcgga ggcagaggcg cgctggtacc
     6481 cgctcgctcc gctgtccgac gtcgcggtca tgctcgcgct ctgtcacgtc ctggtgacgg
     6541 agggcctgca cgacgaggcg ttcctcgcgt cctgctgcca cggagccgac cgcttcatcg
     6601 cctacgtccg cggcgacagc gacggcgtcg cgaagacgcc ggagtgggcg gagcgcatct
     6661 cggggctccc cgccgaggat ctccgcgcac tcgcgcggcg gatggccgcg tcgcggacgc
     6721 tcgtcacggt ctcgttctcg ctccagcgcg cggagcacgg cgagcagccg gtctgggccg
     6781 ggctcgcgct cgccgccctg ctcggccaga tcgggctgcc gggcggtggc ttcggccacg
     6841 gctacggctc gatgggcgag ctcggcagcg cgagcgccgt gatcccgctc cccttcgtgc
     6901 ccgagggcca gaacccggtg aagagcttca tcccggtcgc gcggatcgcg gacctcctgc
     6961 tgcacccggg cgagcccttc gattacgacg gcgcgcgcca cgtctacccc gacgtccgtc
     7021 tcgtgtactg ggccggcggc aacccgttcc atcaccatca ggacctgaac cgcctgcgcc
     7081 gcgcgctcgg ccgccccgat acggtcatcg tgcacgagcc gtactggacg ccgatggcgc
     7141 ggcacgccga cctcgtgttt ccggcgacca cgtcgttcga gcgcaacgac atcggctcgg
     7201 ggcgggccga cgcgcgcatc gtcgcgatgc gccgcgccgt cgagccccac gccgaggcgc
     7261 gcaacgacca cgacatcctc gccggcctcg ccgagcgcct cggcgtcgcc agggcgttca
     7321 ccgaggagcg cgacgagcgg gcgtggctgt cgtcgacgtg ggcgaagctg cgcgcatcgc
     7381 tcgaggcggc cggcatcgcc gcgccggact tcgagacgtt ctggcgggac ggctcgttcg
     7441 ccgttcccga cacggaggcg gagcgcgtgc tgttcgacgc gttccgcgcc gacccggcgg
     7501 gcaacccgct gaagacgccg agcggccgga tcgagctcta ctcggagacg atcgccggct
     7561 tcggctacga cgactgcccg ggacatccgg cgtggctcga caagtcggag gcgctcggct
     7621 cgccgcgcgc gcgccgttac ccgctcgtgc tcgtcgcgaa caacccggcg acgcgcctcc
     7681 acagccagct cgatcacggc gaatacagcc agtcggcgaa ggtgaacggc cgcgagcccg
     7741 tgcgcatcca tcccgaggac gccgcggcgc gcggcatcgc gaacggcgac gtggtgcggc
     7801 tctcgaacga ccgcgggagc tgcctcgcgg gcgcggtggt gacggaggac gtccggcgcg
     7861 gcgtcgtgca gctctcgacg ggggcctggt tcgaccccga cgatcccgcc gccgagatcg
     7921 cgacgtgcgt gcacggcaac ccgaacgtgc tgacgcgcga cgcgggcacg tcgcggctct
     7981 cgcagggctg ctcgggccag cacgcgctcg tcgaggtcga gcggtggaac ggcccgctcc
     8041 cgccggtgcg cgccttcgag ccgcccgcgg tcgccgagcg gagctgacgc cggccaggtc
     8101 aggtctcgta acgcacgcgc gccagtggct tgccgtcttc ggtgacgagc ttgcacagcc
     8161 gcgcggaggt gtagacggac tccgtcttca cccacgccgg tgcactctgt ccgggctcgg
     8221 tgacctcgca ccaaccggcg accagcacgc acacctgcga gtaatcgacg tggcggtcgt
     8281 cgtgggtggt gccgacggac tggaggcact tgagcccggc gatcaccgac gccagcgtcg
     8341 cattggggcc gtgccatccg tagttgatcg cggacggctg tccgttgcgc gcacgactca
     8401 cgtacatctg gttcgtgatc gcccagatct tgccgggatc gccgatgctc ggcacgcggt
     8461 agctgtcgtt gcgcgcccgc atgaacgcgg tcgtcttctg gttgaggtac tggttatgct
     8521 tcgccgcctc gccgacgatc ggcgcgccgg ggtcgcgccc gaccggatcg atcttgcacg
     8581 acacctgctg atagcgtcgc gcgtacagat acggcgtgag caacagacat ggctcgggtg
     8641 ccatgtcgct gcggatcgga gccgtgcgat cgatgaggcc gtcctcggca ccgggcgtgc
     8701 gcggatcggg gtggagcttc agcgcgttcg cgaaggcctg gttctgccgc gccgtgcacg
     8761 gcaggcgaca gagcatgtcg ccatcctgat cgatggggtc ggcttccttg aagcggttcc
     8821 actcggcgag cagatcgcgt tcgctcgcag ggatcttcca cttcaccgcg tcgctgctga
     8881 cccagaaccg ggcgttgaag atgccgctcg cgtccgtgaa cgtggtcgag atcggcttga
     8941 aggagaagag gatcctgcgc ttgtcggtgt cccacgcctg cttcagcgtc gggaacgcag
     9001 cggtcccctc ggtgaggtcg tcctccgacg cgcggccgcg cggcatcgcg tggcccatca
     9061 gctcggcgta gaacaggtcc gcgttgtggc ggtcgcgagc cgacatggtt cggctgcact
     9121 accggcgcgg cctctacgac atcggttcgc atgcacggcg cagcacgctg tacgtcaccc
     9181 cgggcgtcgg gttctccggc gtcacccgcc gccacggcga gagcctgcgc atggcaccgc
     9241 tgcggatggc gaaggccgcg cagcgccggt cacgccgggt cggatccaca gcgctgcgat
     9301 cgatttcatc atggcgtgtg ctctcgtggt cgcctgctcg cggttgtctc gccgtcccct
     9361 acttgtcgaa cttcaccgtg acattgtagc tcgcgccgct gccggtcgct gtgttggtcg
     9421 aggccctcgg cggtgggttc tggatcggtc tcggcggcga tgcgctcggg atcgaggtgg
     9481 gcgtcgatgg cggcctggcg aggtgtgcga ggcgtcgctc ggtcgaccag aacgcaccgg
     9541 tcgcgtcgcg gacaggccgt cacgctctcc ccgcgcgacc gcggcggcgt cgtcgagatc
     9601 gacggcgccg gtcgcgccgc ggaccgccgc ggcggcgagc gcgagatagt gcgactcgtc
     9661 ggtgatcacg gatcgcccag gccgcgcgtc gccgctcagg ccatctcgac gctgggcagg
     9721 aagcgcggcg ggacgcggtg cttggtcttc tgctcgtagg cgaacgcgta gccgagcagc
     9781 ttgccctcgg tgtagggctt gccgaagaac gacaggccga ccggcaggcc acggacctgg
     9841 ccggcgggga cggtgacgtg ggggtagccg gacacggcgg gcagggtcga gcagctgacg
     9901 ccgctgccgg cgtcgccgtt gaccgggtcg atcagccagg cggggccgct ggtcgccgcg
     9961 acgaacgcgt cgagcttgtg ctcggccatc accttgtcga gcagcagggt gcgggtgatc
    10021 tggaggcact tggcgcgcgc gtcgcggtag gccttgtcgg tcagcccggg cttcttgtcg
    10081 gcctcctcga agtgctcctg gccgaacagc gcgagctcgg cgccgcggtt ctggaagttg
    10141 aacgcgatcg cctcggcgag cgagtggacg tgggcatcgg ccttgcgcgc cgcgaggtac
    10201 ttgtcgagat aggccttgag ctcgaacagc agcacctcgg tctcggcagc gtcgagctcg
    10261 ggtgggacga tcagctcggc ctgctcgacc agcacggcgc cggcgtcctt gagatcgccg
    10321 agcgcgccgc gcatgagggc atcgacgccg cgcgagaggc cgaagaagcc cttctgcggc
    10381 acgccgagcc gggcgccggc gagcgccttg gggtcgaggt acttggtgta gtcctcgggg
    10441 gcggcggggc gggccttgtc gggcgccgtg gtgaccggat cgtcgggatc gggaccggcg
    10501 atgacggtga gcagctgggc ggcgtcggtc acggtgcgcg ccatcgggcc gagggtgtcc
    10561 tggctcgccg acagcgggac gacgccggcg cggctgacca ggccgatcgt cggcttgacg
    10621 ccgaccaggc cgttgctgct cgacggcgag acgatcgagc cgtcggtctc gctgccgatc
    10681 gccgcggcgc acaggctggc ggcggcggcc acgcccgagc cgctgctcga gcccgacggc
    10741 gtgcggtcga gcgcgtaggg gttgcggcac tggccgccgc gcccgctcca gcccgacagc
    10801 gacgccttgc cgcggaagtt ggcccactcc gacaggttgg tcttgccgag gatgatcgcg
    10861 ccggcggcgc gcagccgcgc gaccacgaac gcgtccttct cggcgtgcga gccctcgagc
    10921 gcgagcgagc ccgcggtggt ggtcatcttg tcgccggtgt cgatgttgtc cttgaccagg
    10981 atcgggaggc cgtgcagcgg gccgcgcagc ttgccggcct tgcgctcggc gtcgagctgg
    11041 tcggcgatcg agtcggcgtc ggggttgagc tccagcaccg agcgcagcgt cggacccttc
    11101 tggtcgatcg cctcgatgcg ctggcggtac ttggcgacca gcgacttcgc ggtgtcggtg
    11161 ccggcggcca tgcgcgcctg gagatcggcg aacgtgagct cggcgagctc gggggcgggc
    11221 agcgactcgg tcaccgaggc gttggccttg gccttggcct cgagctcggc catcgccttg
    11281 gcgttcgggc tcggcttggg cggcggcggc tgggcgggcc tgggctcctc gcgcgtgccg
    11341 catgcggtga gcgcggcccc ggcgaccagg gtcgcgccgc cgacgaacag atcgcggcga
    11401 tcgaggactc tgtcgactcg gtcgctcgcc ttgtcgctgg gcgtctcgct cgctcgcagg
    11461 gtcggatcct ggctcgcacc ggccggggcg tcgtcgtcga tcatggcggg catcatcgat
    11521 tccgcggtgc cagcgggtca acccaaacgg cgccacccgc gcaggcgacc gcgcggtcgg
    11581 tggtgcggat cccggcgcgg ccgcgactac ccgccggtgg cgcgatggcc tagtctcggg
    11641 accgagggtc catgatcaca ctgcattctg ccccggcgac cgcaggcatg gtggtgcact
    11701 ggctgctgct ggagctggag gtcccacaca cgctgcgcat gctcgacctg gaggcccgcg
    11761 agcacaagca gccggcgtac ctcgcgctca acccggccgg cgtggtgccg acgctggtga
    11821 tcgacggcca gccgatctgc gaggccgccg cgatcgcgct gcacctcacc gatacctacc
    11881 ccgcgcgcgg cttcgcaccg gcgaccggca cgctggcgcg cgcggcgtac taccgctgga
    11941 tgttcttcct cgccaacacg atccagccgg cgttccgcgc ctggttcaat ccgacggagc
    12001 tcgccggcga ggaccacgtc gcggtggtga aggcgcaggc ggaggcccgg atcgcggacg
    12061 gctggaatat cctcgacgcc catctcgcgg cgcacgggcc gtacgtgctc ggcgagcggg
    12121 tcacgacgcc cgacttcatg ctgacgatgc tcatgcggtg gtcgcgccac ttcccgagga
    12181 ccgcgctcga gtggccggcg ctcgcccggc tcgccaccgc gatgaaggcg cggccgacgt
    12241 tcaaggagct gtacgcccgc gaagggctca ccgactgggc ctgatccgcg tttgcgctgc
    12301 ggcgagcgct cagtgtgcgg cgctggaccc ggagccggcc gcttgcgcgg cctcgcgcgc
    12361 cgccctggcc gacgcgaaca ccggacaggt gtcgttccgg atgatccagt ccgcggtgtt
    12421 gaagcacaga tcgatgtcgt ggaccgtgca ctgcgcgccg gagtcgtcga ccagcaacgc
    12481 gtcgtactga tcgcagctca cgtcgatcgc gagcgactcg cccggcgcga ggatatcgcc
    12541 ggagatcagg ttgggtcccc acgtcgtgct accgaccgac gtgacgcgga tctcgacgac
    12601 cgcgaagtcg gactcgttgt ggacattgag ctgcccatgg tcgttgccat cgccgccgca
    12661 accggcgatc gcgatcgagc cgagcgctgc agccacgcaa gcaagaattc gtaggttctg
    12721 catggcagga acatcgagca atgaccgtgc cgttgaggca gattgccgca cgccaagttg
    12781 cacggcagtc ggatggcaaa ccgtggcgag tgcgcacgat ggcagcgcgt gctgtgcgag
    12841 gacagggcgg ctgctgccgg atcgcgccgc ggcgccgccg agatcgacag tggagatacg
    12901 ctgtacaaat acggggatgc gcccgcaaac gcgccgggac ggcgccgcgc aggcgcgccg
    12961 agatcgtgct gcagagacgc gccaatgagc cggaatgtat ctggaaccga agtgacgcgc
    13021 ctcgcccggc tgagcgttca gtgcgtggcg ccggacgcag gggccgcgcc ggcagcgctt
    13081 gggtccttgc tacgggtcct tgtgcgtgaa cgccgcgcag gtgtcgctgc cgatgatcca
    13141 gtccgcgttg ctggagcaca gggcgacgtg atggaggatg ctctgatcac cggcctggtc
    13201 gaccagcaac acgtcgtact gaccgcaggc gacggtgacc gtgagcgacg cgcctggcgt
    13261 gagcgtgccg gagatcaggt tgacccccca cgacgtggtg tcgaccgccg cgacgtgaac
    13321 ctctttgatg ccaacgctgc actggttgtg gaccacgagt gccccgtggg gagggggagg
    13381 gggggcgggg acgcagttgt tgttgctgtc gcagacgcag ttgttgtcgt tgtcgcagtc
    13441 gttgtcgctg ccgcatccaa cgattgccgt cgagccgagc gccaccacgc aagcaagaaa
    13501 tcgtaggttc tgcatggcag gacatcgagc aatggccgtg ccttcggggc agattgccgt
    13561 acgcgaactt gcgcggcagt cagctgccca accgtggtga gtgcgcacga cggcagcgcg
    13621 ttgccatgcg aggacaaggc ggctgctgcc ggatcgcgtc gcggcgctgt ggagatcggc
    13681 agtggagata tgctgtactg gcgcggagat gcgcccccca aaggcgccgg gacggcgccg
    13741 cgcaaacgcg tcgggacggc accgcgacag gggcgccgag cagtgcttca gagacgcgcc
    13801 aggcagccgg cgtgtctctg gaagcgcggt gacggcgctc gtccgcgtgc gcgaagcgct
    13861 ccgtgcagac gtgccagggt gatcgggcgc cacgggcatg tgccggcggc gctatcgacg
    13921 tgcgcccgca ggacgtttcc cccgcacgtg ctgcggcgat gcgccggcga ggtgccggac
    13981 cggcggcgcg ctgcgcgggg cggtggtgac cggcatg
//
LOCUS       DASVOR010000199         4418 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410460,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000199 DASVOR010000000
VERSION     DASVOR010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4418)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4418)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4418
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410460"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1868
                     /gene="accC"
                     /locus_tag="VF516_04500"
     CDS             309..1868
                     /gene="accC"
                     /locus_tag="VF516_04500"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8106963.1"
                     /translation="MSPQLRSIRRVLVANRGEIAVRVIRTCKERGIDTVAVFSEVDRR
                     APHVQLADHAVGIGPAPAAQSYLVIDKILAACRETGADAVHPGYGFLSENEDFAEACE
                     AAGIVFIGPSADAMRKMGSKTEARKTVSAAGTPVVPGDNGPTGDGFPTAEQAVAAARK
                     IGFPVLIKAAAGGGGKGMRLVTSEPEFAAAFDGARREATSAFGDGTVYLEKAIERPRH
                     IEIQVFGDHHGNLVHLGERDCSIQRRHQKVIEESPSPVVSPELRARMGAAAIAAARAV
                     AYVGAGTCEFLLGADGGFYFLEMNTRLQVEHPVTEMIYGLDLVSWQLDVAEGRPLPLT
                     QHELDARRRGHALECRVYAEDPVKFLPSPGKITQLRVPDGPYVRNDAGCYEGAEIPVH
                     YDPMISKLVVWGEDRATALARMRRALDEYRVGGIETNLPFHRRCVRHPAFAAGDFDTG
                     FIARHARELAPTAGEPEITAAVIAAALDTVTTAARGGASIDRAATDRAATPARTAETE
                     ISAWRRQLRVP"
     gene            2082..3689
                     /locus_tag="VF516_04505"
     CDS             2082..3689
                     /locus_tag="VF516_04505"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEX8106964.1"
                     /translation="MAQQTKFVFVTGGVVSSLGKGMVSASIGALMENRGLKVANMKLD
                     PYINVDPGTMNPTQHGEVFVTDDGAETDLDLGHYERFVSTKMSRLNNITTGQVYSSVI
                     GKERRGEYLGSTVQVIPHITDEIKARILRCAEGLDVLVVEVGGTVGDIESLPFLEAVR
                     QLRVELGAQNSVSVHLTLVPYIRAAGEFKTKPTQHSVQKLREIGVQCDVLVVRCEQPL
                     PEDSIKKMAMFCSVATDAVFQSIDVDTVYRLPIMLCEQGLDQKLAALLNIWSRASRLS
                     AWEEVVQRITQPRRKVTVGFVGKYVQLVESYKSLNEALLHGGIANDCRVEIKHIDSEE
                     LEKQGTGALAGYDGILVAPGFGARGTEGKIAAVRYAREGKVPYFGICYGMQMACIEFA
                     RHVCGLERANSTEIDPTTPHPVVDLMPDQRGVTDKGATMRLGSYPCVLKSGTRAAEAY
                     GTTEIAERHRHRWEINNNYRDALERHGMVLSGLSPDGRLVEMIELPQHPYFVGCQFHP
                     EFKSRPSAPHPLFARFIKAAIDRQMTR"
     gene            4011..>4418
                     /locus_tag="VF516_04510"
     CDS             4011..>4418
                     /locus_tag="VF516_04510"
                     /inference="COORDINATES: protein motif:HMM:NF012530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEX8106965.1"
                     /translation="MRQELKLSQQLVMTPQLQQAIRLLQLSRMELADLVHEEMLENPI
                     LDDEMDLDQERSRERDELGSEDQQLHQLEGAGSTETPLEGSVVDGIAQTEIKGDATAV
                     NEIDWENYLDNYSMAPAMPAFRGDGEELPSLEAT"
BASE COUNT          723 a         1497 c         1538 g          660 t
ORIGIN      
        1 gaacttcatc tgggtgccga cggcgagcgg ctccttggtc cggatgaaga ttccgccctg
       61 gctgacatcg accgcgtagc gctcgatgaa ttggtccagc gtctcgctct tgaacttgat
      121 cttgagcgtt accggcgttc gtttcccttg tcgggtgttt gggtcgacca agcgatattc
      181 tccgcactcg tcgcagtcgg ggcgtcggcg ctgcttcgca gcgagcgcaa gacgccgatt
      241 tggctcgatt ctagggcggg ggcgtgcgca atgcaacgcg agcatcgttt tgtgtattgt
      301 ccgcgacgat gtcgccgcag ctccggtcga tccgccgcgt cctggtcgct aaccgaggag
      361 agatcgccgt ccgcgtgatc cgcacctgca aggagcgggg gatcgacacg gtggccgtgt
      421 tcagcgaggt cgatcgccgt gcgccccacg tacagctcgc ggaccatgcc gtggggatcg
      481 ggcctgctcc cgccgcgcag agctacctgg tgatcgacaa gatcctggcc gcgtgccgcg
      541 agaccggggc cgacgcggtg cacccggggt acggattcct gtcggagaac gaagacttcg
      601 ccgaggcctg cgaggccgcg ggcatcgtgt tcatcggccc gtccgcagac gcgatgcgca
      661 agatggggag caagaccgag gcgcgcaaga cggtgtcggc ggctggcaca cccgtggtac
      721 ccggcgacaa tggaccgacc ggtgacggct tccctaccgc cgagcaggcc gtggcggcgg
      781 cgcgcaagat cggcttcccc gtgctgatca aggcggcggc gggcggcggc ggcaagggca
      841 tgcgcctggt caccagcgag cccgagtttg ccgccgcgtt cgatggcgca cggcgtgaag
      901 ccacctcggc cttcggggac gggaccgtgt acctggagaa ggcgatcgag cgcccgcggc
      961 acatcgagat ccaggtgttc ggcgaccacc acggcaacct ggtgcacctg ggggagcgcg
     1021 actgctcgat ccagcgccgg caccagaagg tgatcgagga gtcgccgtcg ccggtggtgt
     1081 cgcccgagct gcgcgcgcgg atgggcgccg cggcgatcgc ggcggcccgc gcggtggcgt
     1141 acgtcggcgc ggggacctgc gagttcctgc tcggcgccga cggcgggttc tacttcctcg
     1201 agatgaacac ccggctccag gtcgagcacc cggtcaccga gatgatctac gggctcgacc
     1261 tggtgagctg gcagctcgac gtcgccgagg gccggccgct gccgctgacc cagcacgagc
     1321 tcgacgcccg gcggcgcggc cacgcgctcg agtgccgggt ctacgccgag gacccggtga
     1381 agttcctgcc gtcgccgggg aagatcaccc agctgcgggt gcccgacggc ccgtacgtgc
     1441 gcaacgacgc cggctgctac gagggcgccg agatccccgt gcactacgac ccgatgatct
     1501 ccaagctcgt ggtgtggggc gaggaccgcg cgaccgcgct ggcccggatg cggcgtgcgc
     1561 tcgacgagta ccgcgtcggc gggatcgaga ccaacctgcc gttccaccgc cgctgcgtgc
     1621 gccaccccgc gttcgccgcc ggcgacttcg acaccggctt catcgcgcgc catgcccggg
     1681 agctggcgcc caccgccggc gagcccgaga tcaccgccgc ggtgatcgcc gccgcgctcg
     1741 acacggtgac cacggcggcg cggggcggcg catcgatcga tcgcgcggcg accgaccgcg
     1801 cggcgacccc tgcgcgcacc gcggagacgg agatctcggc ctggcgccgc cagctgcgcg
     1861 tgccctaggg cggaccggcg gccgttccct gagggtggga tctccgatca tacgcccgga
     1921 ccgccgctga tcaacggcga atcgtctgcg gtgaccgcgg atcaccgtcg attttcgcag
     1981 atcgccggtt cggtcgcgtg tccgacgcgt ccccgattgc gtgaacgggt cgcagtcggt
     2041 agaacgctcc gggcgcacgc ttgtcgcgcc atcgaggagc catggcgcag caaaccaagt
     2101 tcgtgttcgt caccggcggg gtcgtgtcgt cgctcggcaa gggcatggtg tcggcatcga
     2161 tcggcgcgct catggagaac cgcggcctca aggtcgcgaa catgaagctc gacccctaca
     2221 tcaacgtcga cccgggcacg atgaacccga cgcagcacgg cgaggtgttc gtcaccgacg
     2281 acggcgccga gaccgatctc gacctcgggc actacgagcg gttcgtgtcg accaagatgt
     2341 cgcggctcaa caacatcacg accggccagg tctactcgtc ggtgatcggc aaggagcgcc
     2401 gcggcgagta cctcggctcg accgtccagg tcatcccgca catcaccgac gagatcaagg
     2461 cgcgcatcct gcgctgcgcc gagggcctcg acgtgctggt ggtcgaggtc ggcggcacgg
     2521 tcggcgacat cgagtcgctg ccgttcctcg aggcggtgcg ccagctccgc gtcgagctcg
     2581 gcgcgcagaa ctcggtcagc gtccacctga cgctggtgcc gtacatccgc gccgccggcg
     2641 agttcaagac caagccgacc cagcactcgg tgcagaagct gcgcgagatc ggcgtgcagt
     2701 gcgacgtgct cgtggtgcgc tgcgagcagc cgctgcccga ggactcgatc aagaagatgg
     2761 cgatgttctg cagcgtcgcg accgacgcgg tgttccagtc gatcgacgtc gacaccgtgt
     2821 accggctgcc gatcatgctg tgcgagcagg ggctcgacca gaagctcgcc gcgctgctca
     2881 acatctggtc gcgcgcgtcg cggctgtcgg cctgggagga ggtcgtccag cggatcaccc
     2941 agcccaggcg caaggtcacg gtcggcttcg tcggcaagta cgtccagctc gtcgagtcgt
     3001 acaagagcct caacgaggcg ctgctgcacg gcgggatcgc caacgactgc cgggtcgaga
     3061 tcaagcacat cgacagcgag gagctggaga agcagggcac cggcgcgctc gccggctacg
     3121 acggcatcct ggtcgcgccg gggttcggcg cgcgcggcac cgagggcaag atcgcggcgg
     3181 tccgctacgc gcgcgagggc aaggtgccgt acttcgggat ctgctacggc atgcagatgg
     3241 cgtgcatcga gttcgcgcgc cacgtgtgcg gcctcgagcg cgccaactcg accgagatcg
     3301 atccgaccac gccgcacccg gtggtcgacc tgatgcccga ccagcgcggc gtgaccgaca
     3361 agggcgcgac gatgcggctc ggctcgtacc cgtgcgtgct caagtccggc acgcgcgccg
     3421 ccgaggccta cggcaccacc gagatcgccg agcgccaccg ccaccgctgg gagatcaaca
     3481 acaactaccg cgacgcgctg gagcgccacg gcatggtgct gtcggggctg tcgcccgatg
     3541 gccggctggt cgagatgatc gagctgccgc agcacccgta cttcgtcggc tgccagttcc
     3601 accccgagtt caagtcgcgg ccgagcgcgc cgcatccgct gttcgcgcgc ttcatcaagg
     3661 cggcgatcga ccgccagatg acgcgataga tgccgcgttg actggcgacc gaccccatcc
     3721 gctcgcggtc gcccccggcg acctcgcgcg ttgacgtccg gcaatcgacc cggcgcgtgc
     3781 cgctatgctg ctgccgggcc ttgccgtagt cggtgcttgt cctacacaag gagggccggc
     3841 cccgccgcca gccccggatt cgttagcctt tagcaagagc cgggccatta gccctttaca
     3901 agtgaacgct cgcgacgtag gctcttttca agcctcgtac cagcagcgaa ccaagtcttg
     3961 taaatatgct aactgagcga aagaaataag gagtctctat ggccatggag atgcgccagg
     4021 aactgaagct gtcgcagcag ctggtgatga cgccgcagct gcaacaggcg atccggctcc
     4081 tgcagctctc gcgcatggag ctggccgacc tcgtgcacga ggagatgctg gagaacccca
     4141 tcctcgatga cgagatggac ctcgaccagg agaggtcccg cgagcgggac gagctgggga
     4201 gcgaggacca gcagctccac cagctggagg gggcagggtc caccgagacg ccgctggagg
     4261 gctcggtggt ggacggtatc gcccagaccg agatcaaggg cgacgcgacc gcggtcaacg
     4321 agatcgactg ggagaactac ctcgacaact actcgatggc ccccgccatg ccggcgttcc
     4381 gcggcgacgg cgaggagctg ccgtcgctcg aagccacg
//
LOCUS       DASVOR010000200         5036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_235676,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000200 DASVOR010000000
VERSION     DASVOR010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5036
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235676"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..707)
                     /locus_tag="VF516_04515"
     CDS             complement(<1..707)
                     /locus_tag="VF516_04515"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8106966.1"
                     /translation="MYRSSRPCHLLLALVALHGVACKPKSKPGGERTEILIGGTLPLT
                     GAEARIGGFYKEGYDLALDEINRAGGVEVGGKKLPVKLTLLDDTTNQDTAASLADKLI
                     NSDKVDFFLGTYSTALVQRQSTVAEQNQMPYLSGGGAASEIYKRGYKWVFGALAPVEL
                     LGTTLMTWIDEQQKAGKLPKPAKIAVLWENTSHGKDFRKGISDFAAKSGGGYEITIDE
                     SFELNGKDYSALLGKVKA"
     gene            complement(901..1413)
                     /locus_tag="VF516_04520"
     CDS             complement(901..1413)
                     /locus_tag="VF516_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106967.1"
                     /translation="MSELGVIASRNPRDRNKIGSIGVALPGVELRIAPDGELQARGGM
                     TMRVLKANSLIGQVCVVGDNRAYLTALIVLDGQVAPVWAAARGIAYGSFAELTSHPEV
                     RAEVERSIAALNERVSRVENIRKWTILPAEWTAESEELTPTLKLKRRVILQKYAPAIT
                     EMYGEADPLR"
     gene            1382..2176
                     /locus_tag="VF516_04525"
     CDS             1382..2176
                     /locus_tag="VF516_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106968.1"
                     /translation="MRLAITPSSLIPHTSVIGDPDRVEELQHLARRRRAAGERRAHPI
                     EPDPGAERGKDRLGRRALVEQLGRHRLAALLHRHHGARDLQRPEHRAAALLVGVLADP
                     RISTLPRGAVAHRASGTAPGMPRSTAAGPDADQGGCSGSGAARTSKRRINSARYARSS
                     AGGTPAGSSMPSRILISDRRHASVAGRTAAVLGQRCRQRRLDRATGALQEHDVDQIAL
                     LCPADDRGELVRAGRLGAPFDASTPVHAITARRRQPGTRETAWTAL"
     gene            complement(2427..2723)
                     /locus_tag="VF516_04530"
     CDS             complement(2427..2723)
                     /locus_tag="VF516_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106969.1"
                     /translation="MRALTTDMTRRDLRPYFLWDEDVSIGELQQILAGPDGPRRDQLL
                     GKMLREARDIDVWQFVRPVEVAGALERLRRRLGRRYAFWSFLIEGWRRHGLLGA"
     gene            3147..3908
                     /locus_tag="VF516_04535"
     CDS             3147..3908
                     /locus_tag="VF516_04535"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8106970.1"
                     /translation="MKVETRSATRTKGRSEPEQLVLFNARGGKRRGAGRPPKGKRAGS
                     PHKERPFLHARYPVHVVLRVIGAVGNLRRRCVYQAIREATLTTARRDDFRIVQISIQR
                     THIHLLVEADDKLALARGMQGFQISGAKHLNVAISKGKPGPRRRGVVFPDRYHAEIIT
                     SPRQARHALSYVMNNWRKHQEDLRAPMSGWTVDWFSSAAMFPGWAEYGDDPFLWRGPP
                     TYDPLVVYQPKTWLLREGWKKAGVISCREVPSAKQ"
     gene            complement(4226..4543)
                     /locus_tag="VF516_04540"
     CDS             complement(4226..4543)
                     /locus_tag="VF516_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106971.1"
                     /translation="MAADVARLIWDAEPEQVDLRAHCDYVMERVMSRGSWIAMRWLRD
                     MYSSEEMADFLVRKGHRLAPRDLAYWSLIAGIELSSPRGGARAPWAGATHIELRASIL
                     DDA"
     gene            complement(4583..>5036)
                     /locus_tag="VF516_04545"
     CDS             complement(4583..>5036)
                     /locus_tag="VF516_04545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106972.1"
                     /translation="SLWAEAGLVTGSADGTMFQSLSTAIDELGLTAGLAARYHLHRRI
                     TAGARVALGAQRVRAAITDSTGTTAYDHAWGAMASAAAALDVFITTPPRFGLGFRLEA
                     GYVAAQGIALTLHTDRPDDAIPLAMTELSLGHLDLSGPTASLSLLGQF"
BASE COUNT          802 a         1729 c         1738 g          767 t
ORIGIN      
        1 gccttcacct tgccgagcag cgcgctgtag tccttgccgt tgagctcgaa cgactcgtcg
       61 atcgtgatct cgtagccgcc gcccgacttc gcggcgaagt ccgagatccc cttgcggaag
      121 tccttgccgt gcgacgtgtt ctcccacagc acggcgatct tggccggctt tggcagcttg
      181 cccgccttct gctgctcgtc gatccaggtc atcagcgtgg tgccgagcag ctcgaccggc
      241 gccagcgcgc cgaacaccca cttgtagccg cgcttgtaga tctcgctcgc cgcgccgccg
      301 ccgctgaggt acggcatctg gttctgctcg gcgaccgtgc tctggcgctg gaccagcgcg
      361 gtcgagtacg tgccgaggaa gaagtcgacc ttgtcgctgt tgatcagctt gtcggccagg
      421 ctcgccgcgg tgtcctggtt ggtggtgtcg tcgagcagcg tcagcttgac cggcagcttc
      481 ttgccgccga cctccacccc gccggcccgg ttgatctcgt cgagcgccag gtcgtagccc
      541 tccttgtaga acccgccgat ccgcgcctcg gcgccggtca gcggcagcgt gccgccgatc
      601 aggatctcgg tccgctcgcc gcccggcttc gacttgggct tgcaggccac gccatggagc
      661 gcgaccaggg cgagcaggag gtgacacggt cgggagctgc ggtacatggt gttcaccggt
      721 ggggcgacat gcgtttgccg aggaggaatc gcaggcggcg aacgctcgtc gctgcggcca
      781 cggccgtccg aacataagcc agccgctgct gcgatggcga gccggcgtga gccagtcctg
      841 acgacgcagt gcgtcgctgc gatccccggc ggatccggcc gtgaccggcg ccccgcgctg
      901 tcagcggagc ggatccgcct cgccgtacat ctcggtgatc gccggcgcgt acttctgcag
      961 gatgacgcgc cgcttgagct tgagcgtcgg cgtcagctcc tcgctctcgg cggtccactc
     1021 cgcgggcagg atcgtccact tgcggatgtt ctcgacccgc gacacccgct cgttgagcgc
     1081 ggcgatcgag cgctcgacct cggcccggac ctcggggtgc gacgtcagct cggcgaacga
     1141 gccgtacgcg atgccgcggg ctgcggccca caccggcgcg acctggccgt cgagcacgat
     1201 cagcgcggtc aggtacgcgc ggttgtcgcc gaccacgcag acctggccga tcagcgagtt
     1261 cgccttgagc acgcgcatcg tcatgccgcc gcgcgcctgg agctcgccgt cgggcgcgat
     1321 ccgcagctcg acgccgggca gcgcgacgcc gatcgagccg atcttgttcc ggtcccgcgg
     1381 gttgcggctc gcgatcacgc cgagctcgct catcccccac acctcggtga tcggggatcc
     1441 cgatcgcgta gaagaactcc agcacctcgc gcggcgtcgg cgcgccgccg gtgaacgtcg
     1501 tgcacacccg atcgagcccg acccgggcgc ggagcgcggc aaagatcggc tcggccgccg
     1561 cgcgctggtg gagcagctcg gccggcaccg gctcgccgcg ctgctccatc gccaccacgg
     1621 tgcgcgcgac ctccagcgcc ccgagcaccg tgctgcggcg ctgctcgtcg gggtccttgc
     1681 tgatccacgt atctccacgc tgccgcgtgg cgccgtcgcg caccgcgcat cgggcaccgc
     1741 ccccggcatg ccccgcagca cagctgcggg acccgacgcg gatcagggcg gctgcagcgg
     1801 ctccggcgct gccaggacca gcaagcggcg gatcaactcg gcgaggtatg cccgcagctc
     1861 cgccggggga acgccggccg gcagctcgat gccgtcgcgg atcttgatct ccgacaggcg
     1921 ccacgccagc gtcgccgggc gtacagccgc cgtccttggc cagcgctgcc ggcaacgccg
     1981 cctcgaccga gcaaccggcg cgctccagga gcatgacgtc gatcagatcg cgctcctctg
     2041 ccctgccgac gatcgcggtg agcttgttcg cgcggggcgc ctcggggcac cgttcgatgc
     2101 ttcgacaccc gtgcacgcga tcacggcacg gcggcggcag ccgggaactc gggagaccgc
     2161 gtggacggcc ctgtgactgg ctcgtcgagg ctgaccagga cgaccaggac gacccactca
     2221 cagtgcatcg ccccgcgcgg atgctgcctt gtcggattta ttcttccctt cgattcgaga
     2281 gagaacaatc tccgggggga aagcgctctc gcggaggagt caggtcgccg gctgcgtggg
     2341 caactcgaca agcacgacgg ccggatcatc gcgcagtcgg cgctggaaga acgtcgagca
     2401 cctcgtgctg gaacgcggtc agcttgctag gcgccgagga ggccatggcg acgccagcct
     2461 tcgatgagga agctccagaa cgcgtaccgg cggccaagcc ggcgccggag ccgctccagg
     2521 gcgcctgcca cctcgaccgg ccgcacgaac tgccagacat cgatgtcgcg agcctcgcgg
     2581 agcatcttgc caagcagctg atcgcgccgc ggaccgtcgg gccccgccag gatctgctgc
     2641 agctcgccga tcgagacgtc ctcgtcccac aggaagtagg ggcgaaggtc tcgccgggtc
     2701 atgtcggtcg tcagcgcccg catcgacccg cagtatacga tctgcggaca gggtacgtgg
     2761 tcaacagtgt tcgccgccgc ctgccggcag gtggaggagc gaggtcaccc gtcctgcagc
     2821 accgcgccgc tggcgcagac cggatcgcgg caccaattcc ggatcgatcg tcgggcaact
     2881 cgctgcgcca tcacggaaga tcccagcctg cgatcacgtc gtccgccgct gaaccagggt
     2941 atctgcaacc atcggggttg catctgtcag ggatgtcctt ggacagcccg taccctgtgt
     3001 cagggatgtc cttggatcaa agtgtcaagg atctatcagg gctgcacccc ctggccacga
     3061 tgatgtctcc cgatggttac atctctttac caaaaattac ggcgatttca attgactgaa
     3121 tgacgctcca ccttcatgtt cgggctgtga aggtcgagac gcgaagcgcc acccgcacga
     3181 aggggcgatc tgagccggag cagttggtac tgttcaacgc ccgcggcggc aagcggcgcg
     3241 gggcgggtcg cccgccgaag ggcaaacggg ccggctcgcc acacaaggag cgaccgttcc
     3301 tccacgcgag gtaccccgtc catgtcgtgc tgcgggtgat cggcgccgtc ggtaacttgc
     3361 gccgccgctg cgtataccag gcgatccgcg aggccacact gaccacggcg cggcgcgacg
     3421 acttccggat cgtgcagatc agcatccagc ggacgcacat ccacctgctg gtcgaagccg
     3481 atgacaagct cgcgctcgcc agggggatgc agggcttcca gatctcgggc gccaagcacc
     3541 tcaatgtggc gatcagcaag ggcaagccgg gtccgcgccg gcggggcgtg gtgttcccgg
     3601 accgctacca cgcggagatc atcacttcgc cccgacaggc gcggcacgcg ctgtcctacg
     3661 tgatgaacaa ctggaggaag caccaggaag acctgcgcgc gccgatgtcc ggctggacgg
     3721 tcgattggtt ctccagcgcg gcgatgttcc ccggctgggc agagtacggc gacgacccgt
     3781 tcctgtggcg cggccccccg acctacgatc cactcgtcgt gtaccaaccc aagacgtggt
     3841 tgttgagaga gggttggaag aaagctggag tgatctcctg ccgcgaggtg ccgtcggcga
     3901 agcagtgaca ctcggatctc gtacctccgg gaccaccgcg ggttccacgg cgcacgcgca
     3961 cctgaaccta gacctgcatc cgacaacgtc gcccgtgcag gcaccgagcg cctgcgaagg
     4021 ctcggtaagc cggtgctgca ccaggccaac cagccgctgc cgcccgcggc cgtgattcgg
     4081 tgcgacgtgg aaatccggat cagcgactcg gcaggatgga cgtagtttct gcggatgctc
     4141 catcgagcca gtcaaacctg ccattcggat gcaagcaagc gcggaagcat agcagcgtgc
     4201 agagagctcg aacctgaatc acgcttcagg catcatcgag gatggacgcc cgtagctcaa
     4261 tgtgtgtggc cccggcccac ggcgctcgcg cgccgccgcg cggcgaggac agctcgatgc
     4321 ctgcgatgag ggaccagtag gcgagatcgc gcggcgcgag gcggtgaccc ttgcgaacga
     4381 ggaaatcggc catctcctcc gaggagtaca tgtcgcgtag ccagcgcatg gcgatccagc
     4441 tgccgcggga catcacacgc tccatcacgt agtcacaatg tgcgcgaagg tcgacctgct
     4501 cgggctcggc atcccagatc aggcgagcga cgtcggcggc cacaccacag catagcagcg
     4561 gggcgtcggg gcagccccgc ggtcagaact ggccgagcag cgacaggctg gcggtcgggc
     4621 cgctgaggtc gaggtggccg agcgagagct cggtcatcgc gagcgggatg gcgtcgtcgg
     4681 ggcgatcggt gtgcagggtg agcgcgatgc cctgggcggc gacgtagccg gcctcgaggc
     4741 ggaagccgag gccgaagcgc ggcggggtgg tgatgaacac gtcgagcgcg gcggcggccg
     4801 aggccatcgc gccccaggcg tggtcgtagg ccgtggtgcc ggtggagtcg gtgatcgcgg
     4861 cgcggacgcg ctgggcgccg agcgcgacgc gggcgccggc ggtgatccgg cgatggaggt
     4921 ggtagcgcgc ggcgaggccg gcggtcaggc cgagctcgtc gatcgcggtc gacagcgact
     4981 ggaacatcgt gccgtcggcc gagccggtga cgaggccggc ctcggcccac aggctg
//
LOCUS       DASVOR010000201         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_31155,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000201 DASVOR010000000
VERSION     DASVOR010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31155"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            388..720
                     /locus_tag="VF516_04550"
     CDS             388..720
                     /locus_tag="VF516_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HEX8106973.1"
                     /translation="MAAHRLILPLGMRVLVQVIRIEERTDAGLYLPAGAKEAQDEAFY
                     GKVIEVARDRPTTSEVTENVSGVPHGAHVLFRKEAGVRVPWDDRLRLIDVKDILATVE
                     EVNAEEAH"
     gene            725..1354
                     /locus_tag="VF516_04555"
     CDS             725..1354
                     /locus_tag="VF516_04555"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8106974.1"
                     /translation="MSSPPPRSPIPRLDELDLVIATARLRLRPLEDRDVDDLWPLVSD
                     PELPRRMTWAAHTDRSQTLAFLQSTRDRLARGTGVNWAIEVDGRASGSVALGDIRFAV
                     AALRIDDAELGYWIAPALRGAGLVTEAAQAVVRFGFEAIGLHKIHVRCVDDNVASRRV
                     IEKLGFRMVGRLEDDVWRDGRWWSMLLYELTAPEWGDVSTTMRVKRPAP"
     gene            1457..>2230
                     /locus_tag="VF516_04560"
     CDS             1457..>2230
                     /locus_tag="VF516_04560"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX8106975.1"
                     /translation="MDVRCEKCQTEYELDEARLKPGGVTVKCTNCGHMFKIRKRAITN
                     VGITSPPGSPPGASGGPAGSTMPSGLVGHGAAAARARSSSSKPIPPVIRPRGDSLLDD
                     DARPTVVTGSDEAPTTVDRQWLIRLENGEQKSCRELAMLQQWIVSGVVTRESLISRTG
                     KTWKRIGDIADLDQYFAIADEARATRVKPASRPGPIASIKEIPGTIPGYTAAQAAGGT
                     ILPDDDEPEARTTRAYRQGRAMANTPPPVPVRGAARTPAV"
BASE COUNT          327 a          766 c          847 g          290 t
ORIGIN      
        1 accgagaacg tgtccggggt gccgcacggg gcccacgtgc tgttccgcaa ggaggccggg
       61 gtgcgcgtgc cgtgggacga ccggctgcgg ctgatcgacg tcaaggatat cctcgccacc
      121 gtcgaggaag tctcggccga cgaggcgcac tgatggcgtc gccgccgccg cgctccagct
      181 ggaggcgacc ctgcggcgcc agcggctgtg ggggcggacc gagatcgtcg gaccgcggat
      241 cgacgtgcgg tcggggtcct gcgccgaccc ggcgatgcca ccggcaatcg acggcgagcg
      301 cgcagtgctg cgcggcgccg gcttgacccg gctgcgctgc ctggcgcaga gtggcgccgt
      361 cgtcttcgag cgtgatctgt agctaacgtg gccgcccacc gtctgatcct cccactcggc
      421 atgcgcgtcc tggtccaggt gatccggatc gaggagcgca ccgacgccgg gctgtacctg
      481 cccgcgggcg ccaaggaggc ccaggacgag gcgttctacg gcaaggtgat cgaggtcgcc
      541 cgcgatcggc cgaccaccag cgaggtcacc gagaacgtgt ccggggtgcc gcacggggcc
      601 cacgtgctgt tccgcaagga ggccggggtg cgcgtgccgt gggacgaccg gctgcggctg
      661 atcgacgtca aggacatcct cgccacggtc gaggaggtca acgccgaaga ggcgcactag
      721 gcccatgtcc agcccgccgc cgaggagccc gatccccagg ctcgacgagc tcgacctggt
      781 gatcgccacc gcccggctac ggctccggcc gctggaggac cgcgacgtcg acgacctgtg
      841 gccgctggtg tcggatccgg agctgccgcg gaggatgacc tgggccgcgc acaccgatcg
      901 cagccagacc ctggcgttcc tccagtcgac gcgcgaccgg ctcgcccggg gcaccggcgt
      961 gaactgggcg atcgaggtcg acggccgcgc gtcggggtcg gtcgccctcg gcgacatccg
     1021 gttcgcggtc gccgcgctgc ggatcgatga cgccgagctc ggctactgga tcgccccggc
     1081 gctgcgcggc gcggggctgg tgaccgaggc ggcgcaggcc gtggtgcgct tcgggttcga
     1141 ggccatcggg ctgcacaaga tccacgtccg ctgtgtcgat gacaacgtcg cctcgcgccg
     1201 cgtgatcgag aagctcgggt tccggatggt cggccggctg gaggacgacg tgtggcgcga
     1261 cggccggtgg tggtcgatgc tgctctacga gctgaccgcg ccggagtggg gcgacgtgtc
     1321 gacgacgatg cgggtcaagc ggcccgcccc ctgatccgcg ggatcagcgt cgatcgcgcg
     1381 tggggcacga ggtgcacggc gcacaccgcc gtgctacgtt cgcaaggcaa gacacgcggt
     1441 tgcgtgggtc gcaagcatgg acgtccgctg cgagaagtgt cagaccgagt acgagctcga
     1501 cgaggcgcgt ctgaagcccg gtggtgtcac cgtgaagtgc accaactgcg gccacatgtt
     1561 caagatccgc aagcgggcga tcaccaacgt cgggatcacg tcgcctcctg ggtcgcctcc
     1621 gggcgcatcg ggcgggccgg cgggctcgac gatgccgtcg ggcctcgtcg ggcacggcgc
     1681 ggccgcggcg cgggcccgga gcagtagctc caagccgatc ccgccggtga tccggccgcg
     1741 cggtgactcg ctgctggacg acgatgcgcg gccgaccgtg gtgaccggca gcgacgaggc
     1801 accgaccacg gtcgaccggc agtggctgat ccggctcgag aacggcgagc agaagagctg
     1861 ccgcgagctc gcgatgctcc agcagtggat cgtctcgggc gtggtgacgc gcgaatcgct
     1921 gatctcgcgc accggcaaga cctggaagcg gatcggcgac atcgccgatc tcgaccagta
     1981 cttcgcgatc gccgacgagg cgcgcgcgac gcgcgtcaag ccggcgagcc ggccgggccc
     2041 gatcgcctcg atcaaggaga tcccgggcac gatcccgggc tacaccgccg cgcaggccgc
     2101 cggtgggacg atcctgcccg acgacgacga gccggaggcg cgcaccacgc gggcctatcg
     2161 gcagggacgg gcgatggcca acacgccgcc gccggtgccg gtgcgcgggg ccgcccggac
     2221 gccggccgtg
//
LOCUS       DASVOR010000202         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_557296,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000202 DASVOR010000000
VERSION     DASVOR010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557296"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VF516_04565"
     CDS             complement(<1..1150)
                     /locus_tag="VF516_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8106976.1"
                     /translation="MQPSLPPGPRAPSAIQTMGWWSRPSAYMERCRDKYGKRFTIKLM
                     MAPSFVMLSDPDDIKEVFQAPPDVLHPGEGAKVLEPFVGSHSVILLDEQEHLEQRKLM
                     LPAFHGKKMQELESLMTEVAEQEVERWPRDRPIEIHPLTQKLTLEVILRAVFGLDEGE
                     RLDRQRDLITQLANFATRPASLYAEAKNPLVRRLTGWDDYMKVSEESDSLLYDVIHER
                     RASNGDDRPDVMAMFLAARHEDGSPMSDEELRDELTTMVVAGHETTASELAWAFEALP
                     RNPQVLKKLTDEIDADQGDEYLTATINETLRRRPVLPNAEPRLTMQPVTIGDWHYPEG
                     VALVAHAYLLHHDPAIYPDPYAFRPERFLEEQPGTYTFIPFGGGRRRCI"
     gene            1270..1342
                     /locus_tag="VF516_04570"
     tRNA            1270..1342
                     /locus_tag="VF516_04570"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1303..1305,aa:Thr,seq:ggt)
     gene            1471..2505
                     /locus_tag="VF516_04575"
     CDS             1471..2505
                     /locus_tag="VF516_04575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106977.1"
                     /translation="MTQIRDRASALQPTELLAAFFSLAFAFLLAAADSALLYVAADWA
                     PGRWLALHLAFVGGISQLVLGAAQFFAGAFLATDPPPRRLVRVQLVAWNAGAVLVAAG
                     VSTGLSTLAAAGGALLAGGLAGFVVGLESMRRRSLPRAPWAVRWYEAAAVFLAGGVPL
                     GVLLAVGAPWTAGSLLGGHVVLNVGGWFGTAIVGTLHTFFPSLTGTRLAHPQLQRFVF
                     ASWTLGIIALADGYTFGLSAVVIAGWAALTVAAALLATNILASVRVTSEPLSLPARLV
                     AVAQGFLLLALALNLVDALGGQASSPPLHEPTASLILAGWLGLTVAGSLLHLVPVLLR
                     ARRIAPSLPA"
     gene            2502..2951
                     /locus_tag="VF516_04580"
     CDS             2502..2951
                     /locus_tag="VF516_04580"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8106978.1"
                     /translation="MKRDPALSPLSHDHHQALFVAQQLRRATAGSVTEARSAFLAFWN
                     GHGAAHFRLEEDILLPGYAPYGDPHHRLVALALCDHVEIRARAAAVAENSGISAAALN
                     ELGVRLAEHVRLEERELFPLIEAALPPDQLAALGTARAETERELDGG"
     gene            complement(2891..>3152)
                     /locus_tag="VF516_04585"
     CDS             complement(2891..>3152)
                     /locus_tag="VF516_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106979.1"
                     /translation="FKLVAAWFPDQVGGVTGVVGAAGGLGGFFPPLVMAIVKSATGSY
                     LIGFVLMAAVAVACLLVLSRLSTYPPSSSRSVSARAVPSAAS"
BASE COUNT          395 a         1092 c         1103 g          562 t
ORIGIN      
        1 cgatgcaccg acgccggccg ccgccgaacg ggatgaacgt gtaggtgccg ggctgctcct
       61 cgaggaagcg ctcgggccgg aacgcgtacg ggtccgggta gatcgccggg tcgtggtgca
      121 gcaggtacgc gtgcgcgacc agcgcgacgc cttccgggta gtgccagtcg ccgattgtga
      181 ccggttgcat cgtgagccgc ggctcggcgt tcggcagcac cgggcgccgg cgcagcgtct
      241 cgttgatcgt tgccgtgagg tactcgtcgc cctggtcggc gtcgatctca tctgtcagct
      301 tcttcagcac ctgcggattt cgcgggagcg cctcgaacgc ccaggccagc tccgacgccg
      361 tcgtctcgtg gccggccacc accatcgtgg tcagctcgtc gcgcagctcc tcgtccgaca
      421 tcggcgagcc gtcctcgtgt cgcgcggcca ggaacatcgc catcacgtcg ggccggtcgt
      481 cgccgttcga cgcgcggcgc tcgtggatca cgtcgtagag gagcgagtcg gactcctcgg
      541 agaccttcat gtagtcgtcc catcccgtca gccgtcgcac gagcggattc ttcgcctccg
      601 catacaggct cgccggccgg gtggcgaaat tggcgagctg agtgatgagg tcgcgctggc
      661 gatcgagtcg ctcgccctcg tcgagcccga agaccgcgcg caggatcacc tcgagcgtga
      721 gcttctgcgt gagcgggtgg atctcgatcg gccggtcgcg cggccagcgc tcgacctcct
      781 gctcggccac ctccgtcatc aggctttcca gctcctgcat cttcttgccg tggaacgccg
      841 gcagcatgag cttgcgctgc tccaggtgct cttgctcgtc aaggaggatc accgaatgcg
      901 agcccacgaa cggctcgagc accttcgccc cctcgccggg gtggagcacg tcgggcggcg
      961 cctggaagac ctccttgatg tcgtccgggt cggacagcat cacgaacgac ggcgccatca
     1021 tgagcttgat cgtgaagcgc ttgccgtatt tgtctcggca gcgctccatg tacgcgctcg
     1081 gccgcgacca ccagcccatg gtctggattg cgctcggcgc gcgtggcccc ggtggcagtg
     1141 acggctgcat gcggctctcc tccccctgcg ttcgccctgc agggttgata ctcgcgcacg
     1201 ggcccgcggt gtgtgccatt gggcacgaag gacgccgacg ccgttacggc ctagactgcc
     1261 cggtcgagcg ccctcttagc tcagttggta gagcacctcc atggtaagga gggggtcgcc
     1321 ggttcgagtc cggcagaggg cttggcttgg actcgctgct ttgccgggtt ggcggagttt
     1381 gccgctgaag acacatcagt gatttgccgc aagtgcccgg cggctgaggg cggatgccgg
     1441 tcgagcgccc ccagccgatc ctcgtctggc atgacgcaaa tacgcgacag ggcgagtgcc
     1501 ctgcagccga cggagctgct cgccgccttc ttctctctgg cgttcgcctt cctgctggcg
     1561 gcagcggaca gcgccctcct gtacgtcgcg gcggattggg cgcccggccg ctggctggcc
     1621 ttgcatctcg cgttcgtcgg cgggatttcc cagctcgtgc tgggcgccgc gcagtttttc
     1681 gccggcgcct tcctcgcgac ggatccgccg ccccgcaggc tcgttcgcgt gcagctcgtc
     1741 gcgtggaacg ctggtgccgt gctcgtggcc gccggcgttt ccacggggct gagcacgctg
     1801 gcggccgcgg gcggtgcgct gctcgcgggt ggcttggccg gcttcgtcgt cgggctggaa
     1861 tcgatgcgcc gccgttcgct cccgcgggcg ccgtgggcgg tccgctggta cgaggcggcc
     1921 gcggtgttcc ttgccggcgg ggttccactg ggtgtgctgc tcgccgtcgg tgcgccctgg
     1981 accgccggca gcctgctggg cgggcacgtg gtcctcaacg tcggcggctg gttcggcacc
     2041 gcgatcgtcg ggacgctgca cacgttcttc ccgtcgctga cggggactcg gctggcgcat
     2101 ccccagcttc agcgattcgt cttcgcctcg tggacgttgg gcatcatcgc gctggcagat
     2161 ggatacacat tcgggttgtc cgcggtggtg atcgccgggt gggccgcgct cacggtcgcg
     2221 gcggcgctgc tcgccacgaa catcttggcc tctgtgcgtg tgacgtcgga gccgctgtca
     2281 ctgccggcgc ggctcgtggc tgtcgcgcaa gggttcctgc tgctcgccct cgcgctcaac
     2341 ctcgtggacg cgctgggggg ccaagcgtcg tcgccgccgc tccatgagcc gaccgcgtcg
     2401 ctgatcctgg ccggctggct gggcctgacg gtggccggct cgcttcttca cctggtgccc
     2461 gtcctgctgc gcgcgcggcg aatcgcgccg tcccttcccg catgaagaga gatccggcgc
     2521 tcagcccgct ctcgcacgac caccaccagg cgctgttcgt cgcgcagcag ctccgtcgtg
     2581 ccaccgcagg gtccgtgacg gaggcgcgtt cggcgttcct cgccttctgg aacggtcatg
     2641 gggcggccca cttccggctc gaggaggaca tcctcctgcc cggatacgca ccgtatggcg
     2701 atccccatca ccggctggtt gccctcgcgc tgtgcgatca cgtcgagatc cgtgcgcgcg
     2761 ctgcggcggt ggccgaaaac agcggcatct ccgccgccgc gctgaacgag ctgggcgtac
     2821 ggctggcgga acacgtccgg ctcgaggagc gtgagctctt cccgctgatc gaggcggcgc
     2881 tgccccccga tcagcttgcc gcgctgggca cggcgcgggc ggagacggag cgcgagctcg
     2941 acggcggata ggtggacagc ctgctcagca cgagcagaca agcgaccgca accgcggcca
     3001 tcagcacgaa gccgatgaga tagctgccgg tcgccgactt gacgatggcc atgaccagcg
     3061 gcgggaagaa gccgccgagg cccccggccg cgccgaccac gccggtgaca ccgccgacct
     3121 ggtcggggaa ccatgcggcg acgagcttga ag
//
LOCUS       DASVOR010000203         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_381881,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000203 DASVOR010000000
VERSION     DASVOR010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381881"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(423..>1889)
                     /locus_tag="VF516_04590"
     CDS             complement(423..>1889)
                     /locus_tag="VF516_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106980.1"
                     /translation="AAAARPPPAPPRIAAGELPSLERWRHHVTDEILPFWDTPAALGT
                     PVGNFPTYRCNDGTLPRPEALCPELAQPPRWMAGDLDKDFARMKSRQTFLYGVAFHIT
                     GEERYLEYARAGVAWLREHAYERDTGSAVSYWIAGKPGPPPDQRTAQDLAYAQVGLAF
                     YYYLTRDPAVLADLVRLEHTLMQRYWQPSPEPPGGMLRWVLADGDTPGEARREELVSQ
                     LDQINAYMLLLAPLIEDAELAGAWRRDLVLLARVVKDRFFAPEHGLFWGALQDPESRR
                     LGSWHTDFGHSMKSMWMLYLTGQLTGERELVDFARPLVAPLLARAAQPSGCWASGLDA
                     SGALDRGSQWWIFAELDQAAATLAGREPSPAPYAGYLARSYDCWFTRFVDPEHHDVWP
                     GIPPDGTPVSAARGAPLKQHLWKNGYHVMEHALVALITTAGLTGQRLALYYAFAAAPP
                     RARIQPYYFTGAIAGERDHPLLHHPGLHGRRVEFEALH"
BASE COUNT          264 a          670 c          708 g          247 t
ORIGIN      
        1 cacgccgcca cgccggggag cgcggcggtc gccgtcggca gcgcgatcca ggtcaccggc
       61 ccgccgatct cccgcactgt cgccatgtcc accgcgaccc atgtcgagtc gatggtaatc
      121 ggcacgaacg aacgcacgta aagaattctg ccgaccacga actcacaaat ccagcgccgc
      181 cgccgggtta cagccggagc cggtgcgaat cacgccggcc tggtaggcgg cgctaccgat
      241 gcaggacgcg ccgtggctgc gtcgcggctt cctcgcacgc accggcgttc gcgtcgccac
      301 gccgcgcatg gaggatcacg tagcggcgaa acgacggcgg cgaccggccc gatcgccgcc
      361 gcgtcctaca tgggatcctc gggtgcgcgc gatgtcggcg cgctgcccac cccctcgatc
      421 tgtcagtgca gcgcctcgaa ctcgacgcgg cggccgtgca gccccgggtg gtgcagcagc
      481 gggtgatcgc gctcgccggc gatcgcgccg gtgaagtagt agggctggat ccgcgcgcgc
      541 ggcggtgctg cggcgaacgc gtagtacagc gccaggcgct ggccggtgag tccggcggtg
      601 gtgatcagcg cgaccagcgc gtgctccatc acgtggtagc cgttcttcca caggtgctgc
      661 ttgagcggcg ccccgcgggc cgcgctcacc ggcgtcccgt ccggcgggat ccccggccac
      721 acgtcgtggt gctcgggatc gacgaaccgg gtgaaccagc agtcgtagct ccgcgcgagg
      781 tagccggcgt acggcgcggg cgatggctcg cggccggcca gggtcgcggc ggcctgatcg
      841 agctcggcga agatccacca ctgcgagccg cggtcgagcg cgccgctggc atcgaggccc
      901 gaggcccagc agcccgacgg ctgcgccgcg cgtgccaaga gcggcgcgac cagcggccgc
      961 gcgaagtcga ccagctcgcg ctcgccggtg agctggccgg tcaggtacag catccacatc
     1021 gacttcatcg agtggccgaa gtcggtgtgc cagctgccga gccggcgcga ctccgggtcc
     1081 tgcagcgcgc cccagaacag gccgtgctcg ggagcgaaga accggtcctt gaccacgcgg
     1141 gcgagcagca ccagatcgcg gcgccaggcg ccggcgagct cggcgtcctc gatcagcggc
     1201 gcgagcagca gcatgtacgc gttgatctgg tcgagctggg acaccagctc ctcgcggcgg
     1261 gcctcgccgg gcgtgtcgcc gtcggccagc acccagcgca gcatgccgcc cggtggctcc
     1321 ggcgacggct gccagtaccg ctgcatgagg gtatgttcga gccggaccag atccgcgagc
     1381 acggccggat cgcgggtcag gtagtagtag aacgccaggc cgacctgggc gtaggcgaga
     1441 tcctgcgcgg tgcgctggtc cggcggcggg cccggcttgc cggcgatcca gtagctcacg
     1501 gcgctgccgg tgtcgcgctc gtacgcgtgc tcgcgcagcc acgcgacgcc ggcgcgggcg
     1561 tactccaggt agcgctcctc gccggtgatg tggaaggcca cgccgtagag gaacgtctgg
     1621 cgcgacttca tccgcgcgaa gtccttgtcg aggtcgccgg ccatccagcg cggcggctgc
     1681 gcgagctcgg gacacagcgc ctcggggcgc ggcagcgtcc cgtcgttgca gcggtaggtc
     1741 gggaagttgc cgaccggggt gccgagcgcc gccggcgtgt cccagaacgg caggatctcg
     1801 tcggtgacgt ggtgccgcca gcgctcgagg ctcggcagct cgccggccgc gatgcgcggc
     1861 ggggccggcg gtggccgggc cgcggcggc
//
LOCUS       DASVOR010000204         2014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_381885,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000204 DASVOR010000000
VERSION     DASVOR010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381885"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /locus_tag="VF516_04595"
     CDS             <1..548
                     /locus_tag="VF516_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106981.1"
                     /translation="GWRSFLRGVVPMAPFVFGFPAIELATLPGMLAEALGNAPMAYTG
                     LLVATMLGAGVIVQPVTRRLEPVASARLGLLVGAAGVLLGALAVAHQISALLLVVAPI
                     LGTAYGVTMTGGFQAVQRLARPDARGGITGLYYVVAYLGFAAPYLLALAARIAAPPIA
                     LVVTAGLAIASALALPRLPRA"
     gene            841..1392
                     /locus_tag="VF516_04600"
     CDS             841..1392
                     /locus_tag="VF516_04600"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061810 - NAD glycohydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8106982.1"
                     /translation="MARIFIGSSSRMIPLVDEIARCLCSHGHEPLPWRQIFQHGDITI
                     DRLFAQAREVDGAILVFSEDDRIEHRGNTRYQPGGNILIEFGLFLGCLGRRRAIICNH
                     GDNRMPSDLAGLTYLDVGSQTDSIRPEAWDRIGLWAKDLTGITGPFGEDGITQVFQSF
                     PVSDFCQALQRARRLHILQTFIP"
     gene            1489..1896
                     /locus_tag="VF516_04605"
     CDS             1489..1896
                     /locus_tag="VF516_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106983.1"
                     /translation="MAAGLIEAVRKTDDHDDDTYQISILSTLRLAGEILVAVDYEMFW
                     PHLQRLGLSANELPGKVESKFYYPHMTHDSRRQRIGIAPDLTSNRSHADLMTDRLRDR
                     LMLEPARGVEACIRAARRAQGIKRSFYDQWGTG"
BASE COUNT          333 a          657 c          673 g          351 t
ORIGIN      
        1 cgggctggcg cagcttcctg cgcggcgtgg tgccgatggc accgttcgtg ttcgggttcc
       61 ccgcgatcga gctcgcgacg ctgcccggca tgctggcgga ggcgctgggc aacgcgccga
      121 tggcgtacac cgggctactc gtggcgacga tgctgggcgc cggggtgatc gtccagccgg
      181 tgacacggcg gctcgagccg gtggccagcg cgcggctcgg cctgctggtc ggtgccgctg
      241 gcgtactgct cggcgcgctc gccgtcgcgc accagatctc ggcgctgctg ctggtcgtcg
      301 cgccgatcct tggcaccgcc tacggcgtga cgatgaccgg cggtttccag gcggtccagc
      361 ggctggctcg ccccgatgcg cgcggtggca tcaccgggct gtactacgtg gtggcctacc
      421 tcggcttcgc agcgccgtac ctgctcgcgc tcgcagcgcg catcgccgca ccaccgatcg
      481 cgctcgtggt caccgcgggc ctcgcgatcg cgtccgcact ggcgctcccg cgcttgccgc
      541 gcgcttgatg cagtgctgtg aaatcgcgcg gcacgcgcgg gaagcagatg tggatcgtca
      601 tggaagaagc cgacaccgcc cacgccaccc ggaccaacat cgctcgtccc atgccgacaa
      661 ggatgtgggg tgggatacgt ccgggtgtcc tgctcccaca tccgtgaccg atgtggtgct
      721 gccttgtgct gcagagtccc tctctgccgg atcgttaggc ttcgggggga ggattgtaat
      781 gcatggaaat ttgacttcgt cggttctcgt tgcagtgtag gaactggaga gagaggaaca
      841 gtggcaagga tcttcatcgg cagctccagc cggatgattc cgctggtgga tgagatcgcg
      901 agatgtctgt gctcgcatgg acacgagcct ctgccctgga ggcagatctt ccagcacggc
      961 gatatcacca tcgatcgcct gttcgcccag gcacgcgagg tcgacggcgc catcctggtg
     1021 ttctccgaag acgacaggat cgagcaccgg ggaaacaccc ggtatcagcc aggaggcaac
     1081 atcctgatcg agttcggtct gttcctggga tgcctcggca ggaggcgagc catcatctgc
     1141 aatcacggcg acaaccgaat gccctcggat ctcgccggcc tcacctatct ggacgtagga
     1201 agtcagacgg acagcatccg tccagaggct tgggacagga tcgggctgtg ggcgaaggac
     1261 ttgaccggta tcaccgggcc gttcggcgag gacggaatca cccaggtatt tcagagcttt
     1321 ccggtctcgg acttctgcca agcgcttcag cgagcgcgac gactgcacat cttgcagacg
     1381 ttcatcccat agctgacgca tcgcgaacgg ctcgtccgcg acctgctcgc gatcggcgcc
     1441 agccacgatc agatcgtccg ctgtcgcgcc tcggaccaga ccatgagcgt ggccgccggg
     1501 ctcatcgagg ctgtccgcaa gaccgacgac cacgacgacg acacgtacca gatctcgatc
     1561 ctgtcgacgc tgcggctcgc gggagagatc ctcgtcgccg tcgactacga gatgttctgg
     1621 ccccacctgc agcgcctcgg actgtctgcg aacgagctcc cgggcaaggt ggagtcaaaa
     1681 ttctactatc cacacatgac gcacgattct cgcaggcaga gaatcgggat tgccccggac
     1741 ctgaccagca atcgcagtca cgccgacctg atgaccgatc ggctccggga tcgactcatg
     1801 ctggaaccag cgaggggtgt ggaggcctgc atccgcgctg ccaggcgagc acagggcatc
     1861 aagcggagct tctacgacca gtgggggacc gggtagagcg actcctccgg gtcggccccg
     1921 ggtggcgctc cgagatccca ggctgttcac ggatctcgcg cgggcgcccg ccggaccgcc
     1981 gacggctacg ccgccgggcg ggtgtgggat gcgg
//
LOCUS       DASVOR010000205         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_498908,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000205 DASVOR010000000
VERSION     DASVOR010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498908"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1555)
                     /locus_tag="VF516_04610"
     CDS             complement(<1..1555)
                     /locus_tag="VF516_04610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106984.1"
                     /translation="MSSDDTSLVGKEFGNYRLAEVLGKGGTSIVYRGVHKSIGSEVAI
                     KVLRRSGSPAMVKRFFHEAVAASQIRHPGIATVFDYGQADDGVAYLVMELLQGENLGD
                     RLRRRGRLDPIEAARLMRQVCGALIAAHDADIVHRDIKPQNLFVVRDADVVGGERVKV
                     LDFGVAKLLAGDDPADPGDRKMLLGTPLYMAPEQFRGGQNGDRRTDVYQIGAVLYHLI
                     TGRPPFPDIRERMRAAAADAPEIRPSLDGILPGMDGVILRCLQRDPGRRYPDVRALED
                     DLRGCLWEPTNATDQSPTIVQRIPDLRMSEPRRDRKAEPVKVRGAHTLASASVASGFF
                     RAADLGRADLGRADLGRAELGPSRMFKESGEFFQQVRQTFEFYRKHLDEEYRQLSAQI
                     ESAHRLWIACVGVGFVILMAGLVAVLLGAVTQGVVTSSASTVMYFIVRVFQKREDYYR
                     QEKSLKIKYLQYGNDWLLLIQSIEAIPDAAERLEEQHRLARVLLDRIRGEQGPRAPDE
                     PPAAAPRRTE"
BASE COUNT          286 a          692 c          664 g          294 t
ORIGIN      
        1 gctcggtccg gcgcggcgcg gcggccggcg gttcgtcggg cgcgcggggg ccctgctcgc
       61 cccggatccg gtcgagcagg acccgcgcca ggcggtgctg ctcctccagc cgctcggccg
      121 cgtcggggat cgcctcgatg ctctggatca gcagcagcca gtcattgccg tactgcagat
      181 acttgatctt gagcgacttc tcctggcgat agtagtcctc gcgcttctgg aacacccgga
      241 cgatgaagta catcacggtc gaggccgacg aggtcaccac gccctgggtg acggcgccga
      301 gcagcaccgc gaccaggccc gccatcagga tcacgaagcc gacgccgacg cacgcgatcc
      361 acagccggtg cgcgctctcg atctgcgccg acagctggcg atactcctcg tcgaggtgct
      421 tgcggtagaa ctcgaaggtc tgccgcacct gctggaagaa ctcgccgctc tccttgaaca
      481 tgcgcgacgg tccgagctcg gctcgcccga gatccgctcg cccgagatcc gctcgcccga
      541 gatccgctgc acggaagaag ccggaggcga ccgacgccga ggcgagggta tgcgcacccc
      601 gcaccttgac gggctcggcc ttgcggtccc ggcgcggctc ggacatccgc agatccggaa
      661 tccgctggac gatcgtcggt gactggtcgg tcgcgttggt cggctcccac aggcagccgc
      721 ggaggtcgtc ctcgagcgcg cgcacgtcgg ggtagcgccg ccccgggtcg cgctgcaggc
      781 agcgcaggat cacgccgtcc atcccgggca ggatgccgtc gagcgacggc cggatctcgg
      841 gcgcgtcggc cgcggccgcc cgcatccgct cgcggatgtc cgggaacggc gggcggccgg
      901 tgatcaggtg atacagcacc gcgccgatct ggtagacgtc ggtccggcga tcgccgttct
      961 ggccgccgcg gaactgctcg ggcgccatgt agagcggggt cccgagcagc atcttgcgat
     1021 cgcccgggtc cgccgggtcg tcgcccgcga gcagcttggc gaccccgaag tcgagcacct
     1081 tgacgcgctc gccaccgacg acgtccgcgt cgcgcacgac gaacaggttc tgcggcttga
     1141 tgtcgcgatg cacgatgtcc gcgtcgtgcg ccgcgatcag cgcgccgcac acctggcgca
     1201 tcagccgcgc cgcctcgatc ggatcgagcc ggccgcggcg gcgcagccgg tcgcccaggt
     1261 tctcgccctg cagcagctcc atcaccaggt acgcgacgcc gtcgtcggcc tggccgtagt
     1321 cgaacacggt cgcgatgccg gggtggcgga tctggctcgc cgccactgcc tcgtggaaga
     1381 accgcttgac catggccggc gagccggacc gccgcaggac cttgatcgcg acctcgctgc
     1441 cgatcgactt gtggactccg cgatagacaa tgctcgtccc gcccttgccg agcacctcgg
     1501 cgaggcgata gttcccgaac tcctttccga cgagtgacgt gtcgtccgac gacatcggct
     1561 cccccatgat cgaggatacg tctcgatcga cacggcggcc agccggcggc ccgacgaggg
     1621 ctcgtcgagc gaggtggtcg gggcaacgac ataatcagca gttcaaaaat ctgtatgctg
     1681 gtttgctaaa tcggacggcc gagaaccagg caacagacac ttttgccgtc acgaccggat
     1741 gttggccgac tcgagggcgc tatcgccggg gcggctgccg ccgcgctcag ggcacgagct
     1801 cttgcaggaa cgccatgccg tcggccttgc tggcgatcag gccctgcacg agccgcgcgg
     1861 tctgctgcgc catcggcgcg ccgtccctgg cgagcttgcc gagcgcgggc cccgcggcgg
     1921 cgcggccctt gtcgct
//
LOCUS       DASVOR010000206         2060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_60449,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000206 DASVOR010000000
VERSION     DASVOR010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60449"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF516_04615"
     CDS             complement(<1..552)
                     /locus_tag="VF516_04615"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8106985.1"
                     /translation="MRAAAVLALLASLSACKKPGSETDAKAPPPPPEKLDLVTAQEAP
                     SPDVLTLTGVITADQRSEVTADTQGKVVAVLVERGQRVKMGQAVVQLDVRNAALSARE
                     AFANLEAARAQKQLAEEECKREQVLFDKGAITRSDYDRQMTQCTSTLQQVSAAQARAD
                     MINKSVSDGLVRAPFDGQVTEKSV"
     gene            complement(689..>2060)
                     /locus_tag="VF516_04620"
     CDS             complement(689..>2060)
                     /locus_tag="VF516_04620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8106986.1"
                     /translation="PPAPRPAAAPAAPAAVPDDLAAFDHDLDTLFSGGGLTSDQAASR
                     ARAASPTVRRRVAELDAAIAQRQAAELARVPQVRGTASYTRLSKITVAPISFEIAGMP
                     VSFAFPSPLTNAYLAEAQLVVPLSDYLLRFPALIDTAKLGVEAARIDKRSSEVGAGQD
                     ARLAYYEWLRARLQVLIAQRQLVQVQRTLDQVRALAEVQRLSRADLMRVEAQEAEAEQ
                     TVDQLQNLAQLREEQLRILIGAAPGEPLALGEDIRSDVQVAGAAPLGDLVDTARRQRL
                     EFKALDTGIAAKQRQRDAELANSLPHLSAIGIADYADPNQRVFPQVDQFKFTWQVGAQ
                     LSWTINDALVAETTKRRLSAEANELRADRQNLENGTRIEVQSAQQAVSLAQHALATTQ
                     KGLAAAEESYRVRRELLNAERATAVELVDAETELTRARIAALNARVDLRVAQAQLNHA
                     IGNDAK"
BASE COUNT          257 a          731 c          756 g          316 t
ORIGIN      
        1 caccgacttc tcggtgacct ggccgtcgaa cggcgcgcgg accaggccgt cgctcaccga
       61 cttgttgatc atgtcggcgc gggcctgcgc ggcgctgacc tgctgcaggg tcgacgtgca
      121 ctgcgtcatc tggcggtcgt agtcgctccg cgtgatcgcg cccttgtcga acagcacctg
      181 ctcgcgcttg cactcctcct cggcgagctg cttctgggcc cgcgccgcct cgaggttggc
      241 gaacgcctcg cgcgccgaca gcgcggcgtt gcgcacgtcg agctggacca ccgcctggcc
      301 catcttgacc cgctggccgc gctcgaccag caccgcgacc accttgccct gggtgtccgc
      361 cgtgacctcg gagcgctggt ccgcggtgat gacgccggtc agggtcagca cgtccggcga
      421 cggcgcctcc tgggcggtca ccagatcgag cttttccgga ggcgggggag gagctttggc
      481 gtcggtctca ctccctggct tcttgcaggc ggacagcgac gcgaggagcg caagcacggc
      541 ggcggcacgc atgggcggcc tacgatgcac ggcctttgcc acgcggtcca gtgcggggtc
      601 aacgggttag ccacgcgact ttgtacaagt tcccgacagg tgtcggttgc agcctgcctg
      661 gcaagcccag ctgccggcgc gcccgcgctc acttcgcgtc gttgccgatc gcgtggttca
      721 gctgggcctg cgcgacgcgc aggtcgaccc gggcgttgag cgcggcgatc cgggcgcggg
      781 tcagctcggt ctcggcgtcg accagctcga ccgcggtcgc gcgctcggcg ttgagcagct
      841 cgcggcggac ccggtagctc tcctcggcgg ccgccagccc cttctgggtc gtggccagcg
      901 cgtgctgggc gagcgagacc gcctgctgcg cgctctgcac ctcgatccgc gtgccgttct
      961 cgaggttctg gcggtcggcg cgcagctcgt tggcctcggc ggagagccgg cgcttggtgg
     1021 tctcggcgac cagcgcatca ttgatcgtcc agctcagctg cgcgccgacc tgccaggtga
     1081 acttgaactg atcgacctgg gggaacacgc gctggttcgg atcagcgtag tccgcgatcc
     1141 cgatcgccga caggtgcggc agcgagttgg cgagctcggc gtcgcgctgg cgctgcttgg
     1201 ccgcgatccc ggtgtcgagc gccttgaact ccaggcgctg gcgccgggcg gtatccacca
     1261 gatcacccag cggcgcggcc ccggcgacct ggacgtcgga ccggatatcc tcgccgagcg
     1321 cgagcggctc gccgggcgcc gccccgatca ggatgcggag ctgctcctcg cgcagctgcg
     1381 ccaggttctg gagctgatcg acggtctgct cggcctcggc ctcctgggcc tcgacccgca
     1441 tcagatcggc gcgcgacagc cgctggacct cggcgagcgc gcggacctgg tcgagcgtgc
     1501 gctggacctg gaccagctgg cgctgcgcga tcagcacctg gagccgggcg cgcagccact
     1561 cgtagtacgc cagccgcgcg tcctgcccgg cgccgacctc gctcgagcgc ttgtcgatcc
     1621 gggcggcctc gacgccgagc ttcgcggtgt cgatcagcgc cggaaaccgc agcaggtagt
     1681 ccgacagcgg caccaccagc tgggcctcgg cgaggtaggc gttggtcagc ggcgagggga
     1741 acgcgaagct gaccggcatg cccgcgatct cgaatgagat cggcgccacc gtgatcttcg
     1801 agagccgggt atagctggcc gtaccacgga cctgtgggac gcgcgccagc tcggccgcct
     1861 ggcgctgggc gatcgccgcg tcgagctcgg ccacccggcg gcgcaccgtc ggggatgccg
     1921 cccgagcgcg cgacgcggcc tggtcggagg tcagcccgcc gccggagaac agcgtgtcga
     1981 gatcgtggtc gaacgcggcc agatcatcgg gaaccgcggc cggggcggcg ggggcggcgg
     2041 ccggccgggg agccggcggt
//
LOCUS       DASVOR010000207         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148141,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000207 DASVOR010000000
VERSION     DASVOR010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148141"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            330..1235
                     /locus_tag="VF516_04625"
     CDS             330..1235
                     /locus_tag="VF516_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106987.1"
                     /translation="MRAPLLVACTLGCCLLGAASGCEKLLSIQNPVAGDGPGQDGGGS
                     DSSDAGGPPPSSPLLLSEVVLTTVPGTPDPGEMIEIVNTSPDDVDLSTYYLSDSGNYY
                     RLPVTAMVDSTDFIVKFPDSAVIHGHQVITIAIATPTEFLNSYSVLPNYSLRDGSMLT
                     ITINGAPQLTNGGEPIILFQWDGRSDLVRDVDILLAGAPSGSANAFPNKSNVTQDGPD
                     PDMVPSKYAVDLGTIHAQPATPGNGFSTKRIALEDGHEVHNGAGNGPSGDDETSEDTA
                     VTWDGTTANPFTAPTPGQVPAALLR"
BASE COUNT          264 a          581 c          489 g          226 t
ORIGIN      
        1 gagcacctcg gcgaccagcg cgtcggcctc ggcccgcgcg atcacccggc cgccgaccag
       61 gcacacgccg agctcgccca ggcggcggcg cagcagcccc gcgaacgcct cgatcggctc
      121 ggtctccagc gcggtcacca gctcgtggat cacgggccca atgtagctcg atcgtccgga
      181 atcgtccggc ctgggtcgac ggcggcggac gctgcggccg ggcgctgcgt gcgatccctg
      241 cgcaccagca gtgcgaggtc gctcacgccg gaccgcgatt gccggagcgc cgtgcgcgcc
      301 gcgccatcgg ctctgctacc atccggagga tgcgggcgcc gctgctcgtg gcctgtacgc
      361 tcggttgctg cctgctcggc gcggcgtccg gctgcgagaa gctgctgtcg atccagaatc
      421 cggtcgccgg cgatggcccg ggacaggacg gcggggggtc cgacagcagc gacgccgggg
      481 gcccgccgcc gagctcgccg ctgctgctgt ccgaggtggt gctgacgacg gtcccgggga
      541 cgccggaccc gggggagatg atcgagatcg tcaacacatc gcccgacgac gtcgacctct
      601 cgacgtacta cctgtccgac agcggcaact actaccggct gccggtcacc gcgatggtcg
      661 attccaccga cttcatcgtc aagttcccgg acagcgccgt gatccacggc caccaggtga
      721 tcaccatcgc gatcgccacg ccgacggagt ttctcaacag ctacagcgtc ttgccgaact
      781 actcgctccg tgacggctcg atgctgacca tcacgatcaa cggcgcgccc cagctgacga
      841 acggcggcga gccaatcatc ctgttccagt gggacggccg cagcgacctg gtgcgcgacg
      901 tcgacatcct gctcgccggc gcgcccagcg ggagcgccaa cgcgttcccc aacaagtcca
      961 acgtcaccca ggacggcccc gatccggaca tggtccccag caagtacgcc gtcgacctcg
     1021 ggacgatcca cgcccagccg gcgacgccgg gcaacggatt ctcgaccaag cggatcgcgc
     1081 tcgaggacgg ccacgaggtc cacaacggcg ccggcaacgg accgtccggc gacgacgaga
     1141 ccagcgagga caccgcggtg acctgggacg gcaccaccgc caacccgttc accgcaccga
     1201 cgcccggcca ggtgcccgcg gcgctgctgc gatagccacc ggctcaagat gtgtcgatca
     1261 aagcgtttcg atctgggtct cccacctcga ggttgacctc gtcgttgact ccatcgtggg
     1321 ccttgacggc gacgtaggac gtcggcccca ccgtcgacct cgaccttgcc catctcccgt
     1381 cattctcgag gacgattcct cgcaaatgcc ccgggttcac gtccaaggtc gacgacgggc
     1441 tcaacgacta cgtcgccctc caggtcaacg ccctgcgaca acgtcaaggt caagctcaac
     1501 ggcctcggac tcagcgcgag ctcaattttc tacacatctt cagaccttga tctcggggac
//
LOCUS       DASVOR010000208         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_206645,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000208 DASVOR010000000
VERSION     DASVOR010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206645"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF516_04630"
     CDS             complement(<1..481)
                     /locus_tag="VF516_04630"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX8106988.1"
                     /translation="MTRLAQFGDRPAVLAEFASGAALLAGIRVLRDAGVSRFDAFTPY
                     EVAGLHELLGVRRSRLTWIALAAGLSFGGGAYLLQWWINVVDYPLNIGGRPYHSAPAF
                     IPITFEMTVLFAAAAALISAIVLGGLPRLWHPVFEVDGFERASIDRFWLAIGAGDAAL
                     "
     gene            complement(478..1866)
                     /gene="nrfD"
                     /locus_tag="VF516_04635"
     CDS             complement(478..1866)
                     /gene="nrfD"
                     /locus_tag="VF516_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX8106989.1"
                     /translation="MTAQGGALLDELLPGRPGDAELSAQLLSTTWRPVTPGWKLAFAI
                     CGALTVLLFAMIALTVLRGIGVWGNNIPVAWGFGIINFVWWIGIGHAGTFISAILLLL
                     EQPWRTSINRFTEGMTLFAVIQAGLFPLLHLGRPWFAYWLFPYPAEMDTWPQVKSALP
                     WDAAAVSTYFTVSLMFWYLGLVPDLAALRDRAPTRARRIAYGILALGWRGSVQQWRHH
                     KVAYYLLAGLATPLVLSVHSVVSLDFAITGLPGWHSTIFPPFFVAGAILSGFAMVLVL
                     ILPARRVFGLHDVITPRHLDAMAKMVLITSWIVLYSYVTEIFVGWYSGDRYEMYQHLV
                     NRPSGWYAWSFWLMLFCNCLATQVFWSRRMRRSTAALFVVALLVNLGMWLERFEIIVV
                     SLSRDFLPSAWHFFKPTWVDLGLLTGTLGFFGLLFLAFLRLVPFVPVAELKQMRSELA
                     HEHAHVHAEARP"
     gene            complement(1863..>2737)
                     /locus_tag="VF516_04640"
     CDS             complement(1863..>2737)
                     /locus_tag="VF516_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8106990.1"
                     /translation="ITQAHHRLEHRPIVLGGTLPQLRDDGAFRDELARHRGAPASLLG
                     PLAQPLPGPAPALAQWAMSIDLTTCTGCAACVMACAAENNTPVVGRAQVLNKREMHWL
                     RIDRYLEGPADEPVAVVQPMTCQHCERAPCEYVCPVEATTHSPDGLNEMTYNRCIGTR
                     FCSNNCPYKVRRFNWFDYKTRGLHLLASNPDVTVRDRGVMEKCTYCVQRIRRAEIDAR
                     IADRRIGTHEVQTACQAACPAQAIAFGSLTDPASTVSEQRRRPHSYAVLHDQGTEPRT
                     RYLARIRNPGGGRS"
BASE COUNT          466 a          950 c          988 g          333 t
ORIGIN      
        1 cgagcgcggc gtcgccggcc ccgatggcga gccagaaccg gtcgatcgac gcgcgctcga
       61 agccgtcgac ctcgaacacc gggtgccata gccggggcag cccgccgagc acgatcgcgc
      121 tgatcagcgc cgccgccgcc gcgaacagca cggtcatctc gaacgtgatc gggatgaacg
      181 ccggcgccga gtgatacggc cggccgccga tgttcagcgg atagtcgacc acgttgatcc
      241 accactgcag caggtaggcg ccgccgccga acgagagccc ggccgccagc gcgatccagg
      301 tcagccgtga ccggcgaacc ccgagcagct cgtgcaggcc cgcgacctcg tagggcgtga
      361 acgcgtcgaa ccgcgacacg ccggcgtcgc gcaatacgcg gatcccggcg agcagggcgg
      421 cgcccgacgc gaactcggcc agcaccgccg gccggtcgcc gaactgcgcg agccgcgtca
      481 cggccgcgcc tccgcgtgaa cgtgggcatg ctcatgggcg agctcgctgc gcatctgctt
      541 gagctcggcg accggcacga acggcaccag ccggaggaac gcgaggaaca acaggccgaa
      601 gaacccgagc gtgccggtca ggagcccgag atcgacccag gtcggcttga agaagtgcca
      661 cgccgacggc aggaagtcgc gcgacagcga caccacgatg atctcgaacc gctcgagcca
      721 catcccgagg ttgaccagca gcgcgacgac gaacagcgcg gccgtgctgc ggcgcatgcg
      781 gcgcgaccag aacacctggg tcgcgaggca gttgcagaac agcatcagcc agaacgacca
      841 cgcgtaccag ccggacggcc ggttcacgag gtgctggtac atctcgtagc gatcgccgga
      901 gtaccagccg acgaagatct cggtgacgta cgagtacagg acgatccagc tcgtgatcag
      961 caccatcttg gccatcgcgt cgaggtggcg cggcgtgatc acgtcgtgga ggccgaacac
     1021 ccggcgcgcc gggaggatca gcaccagcac catcgcgaac ccggacagga tcgcgccggc
     1081 gacgaagaac ggcgggaaga tcgtcgagtg ccagcccggc aggccggtga tcgcgaagtc
     1141 gaggctgacc accgagtgga ccgacagcac gagcggcgtc gccagcccgg cgagcaggta
     1201 gtaggcgacc ttgtggtgcc gccactgctg caccgagccg cgccagccca gcgcgaggat
     1261 gccgtaggcg atccggcgcg cccgggtcgg cgcgcgatcg cgcagcgccg cgaggtccgg
     1321 gaccagcccg aggtaccaga acatcagcga gaccgtgaag taggtcgaca ccgcggcggc
     1381 gtcccacggc agcgcgctct tgacctgcgg ccaggtatcc atctcggcgg ggtaggggaa
     1441 cagccagtag gcgaaccacg gccggccgag gtggagcagc gggaacagcc cggcctggat
     1501 caccgcgaac agcgtcatgc cctcggtgaa gcggttgatc gacgtccgcc agggctgctc
     1561 gagcagcagc aggatcgccg agatgaacgt gccggcgtgg ccgatgccga tccaccacac
     1621 gaagttgatg atcccgaacc cccacgcgac cgggatgttg ttgccccaca cgccgatgcc
     1681 gcgcagcacg gtcagcgcga tcatcgcgaa cagcagcacg gtcagcgcgc cgcagatcgc
     1741 gaacgcgagc ttccagccgg gcgtgaccgg ccgccaggtc gtcgacagga gctgcgccga
     1801 cagctcggcg tcgccgggcc ggccgggcag gagctcgtcg agcagtgcgc cgccctgcgc
     1861 ggtcatgacc ggcctccgcc cgggttgcgg atccgcgcca ggtagcgcgt gcgcggctcg
     1921 gtgccctgat cgtggagcac cgcgtagctg tgcggccgcc ggcgctgctc ggacacggtc
     1981 gacgccggat cggtgagcga gccgaacgcg atcgcctgcg ccggacacgc ggcctgacac
     2041 gcggtctgga cctcgtgcgt gccgatccgg cgatcggcga tccgcgcgtc gatctcggcg
     2101 cgccggatcc gctgcacgca gtacgtgcac ttctccatca cgccgcgatc gcgcaccgtg
     2161 acgtcggggt tgctcgccag caggtggagg ccgcgggtct tgtagtcgaa ccagttgaac
     2221 cggcggacct tgtacgggca gttgttggag cagaaccgcg tgccgatgca gcggttgtag
     2281 gtcatctcgt tgagcccgtc ggggctgtgg gtcgtggcct cgaccgggca cacgtactcg
     2341 cacggcgccc gctcgcagtg ctggcaggtc atcggctgga ccaccgcgac cggctcgtcg
     2401 gcggggccct cgaggtagcg gtcgatccgc agccagtgca tctcgcgctt gttgagcacc
     2461 tgggcgcggc cgaccaccgg cgtgttgttc tctgccgcgc acgccatcac gcacgccgcg
     2521 cagccggtgc aggtcgtcag gtcgatcgac atcgcccact gcgcgagcgc cggagccggc
     2581 ccggggagcg gctgggccag cggaccgagc agcgaggccg gcgcgccgcg gtggcgggcg
     2641 agctcgtcgc ggaacgcgcc gtcgtcgcgc agctgcggca gcgtgccgcc gagcacgatc
     2701 ggccggtgct cgagccggtg gtgggcctgg gtgatcg
//
LOCUS       DASVOR010000209         3425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_264941,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000209 DASVOR010000000
VERSION     DASVOR010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264941"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VF516_04645"
     CDS             <1..251
                     /locus_tag="VF516_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106991.1"
                     /translation="VRAALGWLAATDRAADRIGLVIAGDLASAVRVLERERERGQAGD
                     GGRILDLVWASVTEEVLGVRSRVERWASRPTATPDLPA"
     gene            385..1350
                     /locus_tag="VF516_04650"
     CDS             385..1350
                     /locus_tag="VF516_04650"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8106992.1"
                     /translation="MNRLACVITSSLAFAGCALDNPDPAEPTAEAPAAVSVIQHVFVI
                     AMENHGTSSIYGNTTHAPYINNTLIPMGGRATNLIDTITSLPSEPHYVWMEAGTNAFS
                     DHTFTNDNNPSSSNSTSSTAHLSTQITAAGSTWLSYQEGLNSTTGACPIAGSGSYAPK
                     HDPFIFFRDVSGSTPSKTNSFCAAHHKALTALATDLTNNTVAAYNFITPNLCNDMHDS
                     CSPVNDPIKQGDNWLSSNMPALINYVNAHQGVVFVLWDEPEGSTGNIPLLVIGPNVKA
                     NFASTVRYTQGSLVRSVEAILALPVLSKVSAENTFTDFFNAGFFP"
     gene            complement(1405..2790)
                     /locus_tag="VF516_04655"
     CDS             complement(1405..2790)
                     /locus_tag="VF516_04655"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8106993.1"
                     /translation="MRTPPHCVIPVLLLLIGAAGCQKASKDQAAAGGSGQGSGQTVAQ
                     AAGSGSGSGAGMAVVGKGSGSAAPPVEAKDIDSKDILARTETAPEVQVKHVLLAWNGM
                     AMPRGQLDPRAAKRSNAETAALAQDIATKLKANPDAIEDLAKQYSEDPGSATNGEAYT
                     VKASSQFVPEFKNLALRLKEKEVGIVKSRFGYHVIERVLPPPPDPLESADVLARPAET
                     GPVFVQQVLIGWKDTMLAKSGQADKRANDRTKVDADKLVKEVVDKARAPKADMAKLMK
                     QYSEDPTAKDSGRVDQITADMQVNELFDNFKKLVLRLKVDEIGVVKSPLGWHVVKRVP
                     PPPPDPLESAAILKREPQTQSAKVKHILLGWTGAHPPDDEKGAKRDRATLDKLVKDTV
                     AKLTKGAKIEPLMAQLSEDPGSAQDGKSYDVTPTAGLVPPFKNLSLRLKVGEVGVVKS
                     DYGIHIIQRVE"
     gene            3100..>3425
                     /locus_tag="VF516_04660"
     CDS             3100..>3425
                     /locus_tag="VF516_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106994.1"
                     /translation="MPPKLPPATSRLGYLRGAVASPVCVAVTLFAACLGIGYAGVLGA
                     IIAAVLVVVLGANASRYRRVRRYLDEQNRAQVRAKRHCRRLKQVRRTSPARMRHYDEL
                     RVMVDE"
BASE COUNT          505 a         1244 c         1116 g          560 t
ORIGIN      
        1 cggtgcgcgc ggcgctcggc tggctggccg cgaccgatcg cgccgccgac cggatcggcc
       61 tggtgatcgc cggcgatctg gcgagcgcgg tccgcgtgct ggagcgcgag cgcgagcgcg
      121 gtcaggccgg cgacggcggc cggatcctcg atctggtgtg ggcaagtgtc accgaggagg
      181 tgctcggcgt gcgcagccgg gtcgagcgct gggcatctcg ccccaccgcg actcccgatc
      241 tgcccgcgtg accgatctcg gcgcacggat ttcgtggcgc cgactacgca gctgagcact
      301 cgcatctgtg tcttctaaca aatgacacac acgtgggacg catgcgccgc gcgcgacatg
      361 ataatgcgag cgcgggagga actcatgaac cgactcgcct gtgtcatcac cagcagcctc
      421 gcgttcgccg gctgcgcgct cgacaatccc gatcccgccg aaccgaccgc cgaggcgccc
      481 gccgccgtgt ccgtgatcca gcacgtgttc gtgatcgcga tggagaacca cggcacgtcc
      541 agcatctacg gcaacacgac ccacgcgccg tacatcaaca acacgctgat cccgatgggc
      601 ggccgcgcca ccaacctgat cgacacgatc acctcgctcc cgagcgagcc ccactacgtg
      661 tggatggagg ccggcaccaa cgcgttctcc gaccacacgt tcaccaacga caacaacccg
      721 tcgtcgtcca acagcacgtc cagcacggcc cacctgtcga cccagatcac cgccgcgggc
      781 tcgacctggc tgagctacca ggagggcctc aacagcacca ccggcgcctg tccgatcgcc
      841 ggcagcggca gctacgcgcc caagcacgac ccgttcatct tcttcaggga cgtgtcgggc
      901 tcgacgccgt cgaagaccaa ctcgttctgc gccgcgcacc acaaggcgct gaccgcgctc
      961 gcgaccgacc tgaccaacaa cacggtcgcc gcctacaact tcatcacgcc caacctgtgc
     1021 aacgacatgc acgacagctg ctcgccggtg aacgatccga tcaagcaggg cgataactgg
     1081 ctgtcgtcga acatgccggc gctgatcaac tacgtcaacg cacaccaggg cgtggtgttc
     1141 gtgctgtggg acgagccgga gggctcgacc ggcaacatcc cgctgctcgt catcggaccc
     1201 aacgtcaagg ccaacttcgc cagcacggtc cgctacaccc agggctcgct ggtccggtcg
     1261 gtcgaggcga tcctcgccct gccggtcctg tccaaggtca gcgccgagaa cacgttcacc
     1321 gacttcttca acgccgggtt ctttccctga ccggccctga ccggccctga ccggcccctg
     1381 atcggccccc gatctggacg gtgcctactc gacgcgctgg atgatgtgga tgccgtagtc
     1441 gctcttgacc acgccgacct cgccgacctt gagccgcagt gacaggttct tgaacggcgg
     1501 caccagcccg gcggtcggcg tcacgtcgta ggacttgccg tcctgggccg agcccgggtc
     1561 ctcggacagc tgggccatca gcggctcgat cttggcgccc ttggtcagct tggccacggt
     1621 gtccttgacc agcttgtcca gcgtcgcgcg gtcgcgcttg gcgcccttct cgtcgtcggg
     1681 cgggtgcgcc ccggtccagc cgagcaggat gtgcttgacc ttggcggact gggtctgcgg
     1741 ctcgcgcttg aggatcgcgg ccgactccag cggatcgggc ggcggcggcg gcacccgctt
     1801 gacgacgtgc cagccgagcg gcgacttgac cacgccgatc tcgtcgacct tgagccgcag
     1861 cacgagcttc ttgaagttgt cgaacagctc gttgacctgc atgtcggcgg tgatctggtc
     1921 gacccggccg ctgtccttgg cggtcggatc ctcggagtac tgcttcatca gcttggccat
     1981 gtcggccttg ggggcgcgcg ccttgtccac cacctccttg accagcttgt cggcgtcgac
     2041 cttggtccgg tcgttggcgc gcttgtcggc ctgcccgctc ttggccagca tggtgtcctt
     2101 ccagccgatc agcacctgct ggacgaacac cggaccggtc tcggcgggcc gcgccaggac
     2161 gtcggcggac tccagcggat cgggcggcgg cggcagcacc cgctcgatca cgtggtaccc
     2221 gaaccggctc ttgacgatgc cgacctcctt ctccttgagc cggagcgcga ggttcttgaa
     2281 ctcgggcacg aactgcgagc tggccttcac cgtgtaggcc tcgccgttgg tcgccgagcc
     2341 cgggtcctcg gagtactgct tggccagatc ctcgatcgcg tccgggttgg ccttgagctt
     2401 ggtggcgatg tcctgggcca gcgccgcggt ctcggcgttg ctgcgcttgg cggcgcgcgg
     2461 atcgagctgg ccgcgcggca tcgccatgcc gttccatgca agcaacacgt gcttgacctg
     2521 gacctccggc gcggtctcgg tccgcgccag gatgtccttg ctgtcgatat ccttggcctc
     2581 gaccggcggc gccgccgaac ccgagccctt gccgaccacc gccatgcccg cgccggagcc
     2641 ggacccggac ccggccgcct gggccacggt ctggcccgag ccctggccgg agccgcccgc
     2701 ggcagcctga tccttggagg ccttctggca gccggcggcg ccgatcagaa ggaggagcac
     2761 gggaatgaca cagtgaggag gggtccgcat ccgggcggca tcctagtccc aagtgcgggc
     2821 cgggcgggcg aaccgggtgt gaattccacg gaccggctgc cgattccacc agaccggccg
     2881 gccgctgccc atgagttgcg gccgcggtca cgcgcggtcc cccaccagcc gaccgccgcc
     2941 gccccgcagc caccgcacgg cgcgcaactg ccacatcgtg gcgcgggcga ccagtgcgag
     3001 cggccgcaac cgtgccgtcg gtgaatgact gaactcgcgc acagcgtggc acgcgggttg
     3061 tagctgctcc ctttcatgcg ccagcctcac cttgtcgtcg tgcctccgaa gctccctcca
     3121 gccacgtccc ggctgggtta ccttcgcggc gcggtcgcca gtccggtgtg cgtcgcggtg
     3181 acgctgttcg cggcctgcct gggcatcggc tatgccggtg tgctcggggc gatcatcgcg
     3241 gccgtgctcg tggtcgtgct cggtgcgaac gcgtcgcgtt accgccgggt ccggcgctac
     3301 ctcgacgagc agaaccgcgc ccaggtccgg gccaagcgcc attgccggcg gctcaagcag
     3361 gtccggcgga ccagcccggc gcggatgcgg cactacgacg agctccgcgt gatggtcgac
     3421 gagat
//
LOCUS       DASVOR010000210         5534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_557360,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000210 DASVOR010000000
VERSION     DASVOR010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5534
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557360"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VF516_04665"
     CDS             <1..590
                     /locus_tag="VF516_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106995.1"
                     /translation="PGQERGGCRPDRTCDVGLLCLSNLCVRPPPADCQDVGEQLASIE
                     LGNYAEPEARAPVVARYKAACETARLSREEQQCLDRSRDRWSAAQCAPRMFPDLASSS
                     TGDCGAIVDRVRAAMQKQASYLGDPRMKTWFDRTMAVMQESCEQDHWPDALKKCMLAG
                     DGLAAMTQTCNQQTPPALQQRLQERLSQAMQDTLR"
     gene            1187..3103
                     /locus_tag="VF516_04670"
     CDS             1187..3103
                     /locus_tag="VF516_04670"
                     /inference="COORDINATES: protein motif:HMM:NF016071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HEX8106996.1"
                     /translation="MKESRYMHARSLPLIATLSALTVVGCMAEATSPDPNNDVTITDH
                     VHQMPFRDSDSHDGATAPPAPVAPAGAQLAYHGGKVIQNVNITKVLYGSGTYIPELTA
                     TTGVNMVSAYTQMVTSGVFDWLTEYNTTSPAQTIGRGTVGPSVQITPAASRNGTTIDD
                     SAIQAEIAAQINSGALPPPTNNQIYMVHFPSGKTITQGGTSSCVSGGFCAYHGTFTIG
                     SQNVYYGVLPALTGGCATGCGGSTTFQNTQSVSSHELIEAVTDAEVGLATVIGPPLAW
                     YDPNNGEIGDICNGQQGTFTGTDGNTYTIQKEFSNQQNDCITTRTVNTNPDFSVSLSP
                     ASTTVAAGGTASVTVSTAKINNSAQSITLSVSGLPSGVTGSFNPATVTAGGNSTLTLT
                     ASSSATGSATYTVTGTSGTTSHTASGSVTVSTGGGGGGGTLTNGVPVSNLSGASGAQQ
                     QFSIVVPAGQALLTVKISGGTGDADLYVRAGSPPTLQTFDCRPFITGNSETCTFNNPA
                     ANTYFVMLNGFTAYSGVTLVATFGADTTTALGNNVPVTGLSGASGSQTFFKLTVPSGQ
                     TRVVFSISGGTGDADLYVKAGAKPTTSTFDCRPFLTGNTETCTFNNPAAGDYFVMIRG
                     FAAYSGVTLVGHFP"
     gene            3702..5474
                     /locus_tag="VF516_04675"
     CDS             3702..5474
                     /locus_tag="VF516_04675"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8106997.1"
                     /translation="MTKLQDGRWDELSEPEISIGSIIQQAHSEDLTGNIIGERFGKYL
                     LVGEIAVGGMAEVFLAVHKGVESFIKVVVIKRVLPHLSNNPEFVRMFVDEARLAARIE
                     HPNIVRTYEFGEVNGQYFTAMEYLPGEDLFKALNNLSMSRQLMPLHIAAGIGVQVCNG
                     LHFAHQFTDTAGKPLALVHRDINPANIIITYGGEVKIIDFGVAKTNANVQTINGVIKG
                     KVAYMPPEQVLGREVDQRADIFSAGVVLWEILTGRPLFLRSNEAATLYAIMNAPIPPP
                     SKLRPDVPPELDKIVTRALARAPGDRYGTAEEMAGALDDVLTRLPRFDARVVASKLEE
                     LFGSTRAEAKRSIAQTRSLTRNISLVMKLRSEVRAELAERLDAVVGDQARQSQPGASG
                     APTARGSGIPVQAVGPSRSQRGLAIALTVVLLAAIASGLAYTMSSRQDRPPAPKQVAA
                     SALLVESTPPGAAVFVSGEPTGLKTPTTLTGITRPQLEVRLELSGYATVIRSVTIPAG
                     TNASAKVTLLPVQGRLVISDLPANASVVVDGQDYDAGEVIPVAAGKHDIRIMLDGKTV
                     AQQSIETASGDQGWKLVRGKLVRN"
BASE COUNT          965 a         2000 c         1774 g          795 t
ORIGIN      
        1 agccgggcca ggagcgcggt ggctgccggc ccgaccggac ctgcgacgtg ggcctgctct
       61 gcctgtcgaa cctgtgcgtg cggccgcccc cggcggattg ccaggacgtc ggcgagcagc
      121 tcgcgtcgat cgagctcggc aactacgccg agcccgaggc ccgggcgccg gtcgtcgccc
      181 gctacaaggc ggcgtgcgag accgcgaggc tgtcgaggga ggagcagcag tgcctcgacc
      241 ggagccgcga ccgctggagc gccgcccagt gcgcgccgcg gatgttcccc gacctggcgt
      301 cgagctcgac cggcgactgc ggcgcgatcg tcgaccgggt ccgcgccgcg atgcagaagc
      361 aggcgtcgta cctcggcgac ccccggatga agacctggtt cgaccgcacg atggccgtca
      421 tgcaggagtc ctgcgagcag gaccactggc cggatgcgct caagaagtgc atgctggccg
      481 gcgacgggct ggcggcgatg acccagacgt gcaaccagca gacgcctccg gcgctgcaac
      541 agcgcttgca ggagcgcctg agccaggcga tgcaggacac cctgcgctga cattccagaa
      601 ttcggccctg gcgcgaagca ccaggcccgg ccgcgaagcg ccacggggga cctgcgaagc
      661 agctcaggac gaaccgagcg accgcgagga atctgatttt cagttatcaa atcggtcaga
      721 tacctgcacc gatgtaggct atcgctcacc ggcggcgtac ctctgactag cggcaaatcc
      781 acccgccacc tccccgtcgc gaccgcgggg gatgcgacag gctgtcactg ctttctgcaa
      841 tcgtcttgca aatagcatgg acgccggcat cgcatcgcgt ccgtgtcgca gatcgcggcc
      901 gtctcgcgtc gggtcccacg cggcgccctg ttcctgaaac tgaatgcagc aaacgtgggt
      961 tggattaccc gctgttctcc gggggaacgg tgggtacacg gctgccgtcg acggtttccg
     1021 gaggcgcggg tcgcagctcg cctcagcgat ccatcgcgtc cgacggacga cctcgcgttg
     1081 cgcatgttgt gcatcgccac ctggcaccca gcatgaagac ccggcgctgc gtcgcgaccg
     1141 gtgtcccgac gagcgacgag ccaacgcgct cggacgctgc ccacccgtga aggagtcccg
     1201 ttacatgcac gcgcgctcgc tccccttgat cgccaccctc agtgccctga ccgtggtcgg
     1261 gtgcatggcg gaggcaacct cgcccgaccc gaacaacgac gtgaccatca ccgatcacgt
     1321 gcaccagatg ccattccgcg acagcgacag ccacgacggg gccacggcgc cgcccgcgcc
     1381 ggtcgccccc gcgggcgcgc agctggccta ccacggcggc aaggtgatcc agaacgtcaa
     1441 catcaccaag gtgctctacg gctcgggcac gtacatcccg gagctcacgg cgaccaccgg
     1501 cgtgaacatg gtgagcgcgt acacccagat ggtgaccagc ggcgtgttcg actggctcac
     1561 cgagtacaac accacgtcgc ccgcccagac catcggccgc ggcacggtcg gcccgtcggt
     1621 ccagatcacg ccggccgcgt cgcgcaacgg caccacgatc gatgactccg cgatccaggc
     1681 cgagatcgcg gcgcagatca actccggcgc gctgccgccg cccaccaaca accagatcta
     1741 catggtgcac ttcccgtcgg gcaagaccat cacccagggc ggcacgtcgt cgtgcgtctc
     1801 gggcgggttc tgcgcgtacc acggcacgtt cacgatcggc tcgcagaacg tgtactacgg
     1861 cgtgttgccc gcgctgaccg gcggctgcgc caccggctgc ggcggcagca ccacgttcca
     1921 gaacacccag tcggtgtcgt cgcacgagct gatcgaggcg gtcaccgacg ccgaggtcgg
     1981 cctcgccacc gtgatcggcc cgccgctcgc ctggtacgac ccgaacaacg gcgagatcgg
     2041 cgacatctgc aacggccagc agggcacgtt caccggcacc gacggcaaca cctacacgat
     2101 ccagaaggag ttctcgaacc agcagaacga ctgcatcacc acccgcaccg tcaacaccaa
     2161 cccggacttc agcgtctcgc tgtcgcccgc gagcacgacg gtcgcggccg gcggcacggc
     2221 gagcgtgacg gtgtcgaccg ccaagatcaa caacagcgcc cagtcgatca cgctgtcggt
     2281 cagcggcctg ccgagcggcg tcaccggctc gttcaacccg gcgaccgtca ccgcgggcgg
     2341 caactcgacc ctgacgctga ccgcgtcgtc gagcgcgacc ggctcggcga cctacacggt
     2401 caccggcacg tcgggcacga cctcgcacac cgccagcggc tcggtgacgg tgtcgaccgg
     2461 cggtggcggc ggaggcggca cgctgaccaa cggcgtcccg gtcagcaacc tgtccggcgc
     2521 cagcggcgcg cagcagcagt tctcgatcgt ggtgcccgcc ggccaggccc tgctgaccgt
     2581 caagatcagc ggcggcaccg gcgacgccga cctctacgtc cgcgccggct cgcccccgac
     2641 cctgcagacc ttcgactgcc gcccgttcat caccggcaac agcgagacct gcacgttcaa
     2701 caacccggcg gcgaacacct acttcgtcat gctcaacggc ttcaccgcgt actcgggcgt
     2761 gaccctggtc gccacgttcg gcgccgacac caccacggcg ctcggcaaca acgtcccggt
     2821 caccggcctg tccggcgcgt cgggctcgca gaccttcttc aagctgaccg tgccgtcggg
     2881 ccagacccgc gtggtgttct cgatcagcgg cggcacgggc gacgccgatc tctacgtcaa
     2941 ggccggcgcc aagccgacca cgtcgacgtt cgactgccgc ccgttcctca ccggcaacac
     3001 cgagacctgc acgttcaaca acccggccgc cggcgactac ttcgtgatga tccgaggctt
     3061 cgcggcgtac tcgggcgtca ccctggtcgg tcacttcccg tgatcgtgac gctgggctga
     3121 ccagcgccgg cgccaccggc gcctgccccg gaatatcgat ctagaacgat ctccgatcga
     3181 cgtcgatcta ttccacaatt ccttataggg aacctggtaa ccgaggcacc ggaggcgcgg
     3241 acacgaccag ccccgtcgcg cgcaggtcga tgcggcgccc gcgcgcggaa gctcgggctg
     3301 caggcgcgcg gcgccgcgtg tgatcgaccg cgcgccggat ggcagcgtcg cctcggtgcc
     3361 gaccggggag cttgctctca cgaaggcacc cgggagcata cacctgacga cgcatgctcc
     3421 cggggacgca tacacctggc gacgctgtcc gacgaggcgg agctcctacc cgatcgctac
     3481 ccgatcggtg ttccattgac ggtccaggaa gcgaaatttc ctccggggct ggacgcgttt
     3541 tccaccgaac accacgagat cagcgctcag cggatcgcat gaggtgcgtt tactgcccac
     3601 gcgcgacgat gtcaggagct gtacggccac cgtcgacgct gagatcctcg atagatcggg
     3661 cgtgcgcttc cgagacacgt gttaggctca ggcggtttcc gatgacgaaa ctacaagacg
     3721 gccggtggga tgagctgtcg gagcccgaaa tttctattgg ctcgatcatc cagcaggccc
     3781 attcggaaga cctcacgggc aacatcatcg gcgagcggtt cggaaagtac ctgctcgtcg
     3841 gtgaaattgc cgtgggcggc atggccgagg tgttcctcgc cgtccacaag ggcgtcgaga
     3901 gcttcatcaa ggtcgtcgtc atcaagcgcg tcctcccgca cctgagcaac aaccccgagt
     3961 tcgtgcggat gttcgtcgac gaagcccggc tcgcggcgcg catcgagcac ccgaacatcg
     4021 tccggaccta cgagttcggc gaggtcaacg gacagtactt caccgcgatg gagtacctcc
     4081 ccggtgaaga cctgttcaag gcgctcaaca atctctcgat gtcgcggcag ctgatgccgc
     4141 tccacatcgc ggccggaatc ggcgttcagg tgtgcaacgg cctccacttc gcacaccagt
     4201 tcacggatac cgccggcaaa ccactcgccc tcgtacaccg cgatatcaat ccggccaaca
     4261 tcatcatcac gtatggcggc gaggtgaaga tcatcgactt cggcgtcgcc aagaccaacg
     4321 cgaacgtgca gacgatcaac ggcgtgatca agggcaaggt cgcgtacatg ccgcccgagc
     4381 aggtgctcgg ccgcgaggtc gaccagcggg cagacatctt ctcggccggc gtggtgctgt
     4441 gggagatcct gaccggccgg ccgctgttct tgcgcagcaa cgaggccgcc acgctgtacg
     4501 cgatcatgaa cgccccgatc ccaccgccga gcaagctgcg ccccgacgtg ccgcccgagc
     4561 tcgacaagat cgtgacgcgc gccctcgccc gcgcccccgg ggatcgctac ggcaccgcgg
     4621 aggagatggc cggcgcgctc gacgacgtgc tgacccgctt gccgcggttc gacgcgcgcg
     4681 tggtcgcgag caagctcgag gagctgttcg gctcgacccg ggccgaggcc aagcgctcga
     4741 tcgcgcagac ccgctcgctg acccggaaca tctcgctggt catgaagctg cgcagcgagg
     4801 tccgcgccga gctggcggag cggctcgacg cggtggtcgg ggaccaggcc cgccagtccc
     4861 agcccggcgc gagcggcgcc ccgaccgccc gcggatcggg gatcccggtc caggcggtcg
     4921 gtccgtcgcg atcgcagcgc gggctcgcca tcgcgctcac cgtggtcctg ctggccgcga
     4981 tcgcgtccgg cctggcctac acgatgtcgt cgcgccagga ccgcccgccg gcgcccaagc
     5041 aggtcgcggc atcggcgctg ctggtcgaga gcaccccgcc cggggccgcg gtgttcgtca
     5101 gcggcgagcc gaccggcctc aagaccccga ccacgctgac cggcatcacc aggccacagc
     5161 tcgaggtccg cctggagctg tccggctacg ccacggtgat ccggagtgtc accatccccg
     5221 cgggaacaaa cgcctcggcc aaggtgacgc tcctgcccgt gcagggtcgg ctggtgatct
     5281 cggacctccc ggccaacgcc agcgtcgtgg tggacggcca ggactacgac gccggcgagg
     5341 tcatcccggt ggcggcgggc aagcacgata tccgcatcat gctcgacggc aagaccgtgg
     5401 cccagcagtc gatcgagacc gcctccggcg atcagggctg gaagctcgtc cgcggcaagc
     5461 tcgtccgcaa ctgacccact cgactacaga tacggatata cggcgagcat gtatacagac
     5521 cttcttctag tagg
//
LOCUS       DASVOR010000211         1702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_264980,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000211 DASVOR010000000
VERSION     DASVOR010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1702
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264980"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF516_04680"
     CDS             <1..703
                     /locus_tag="VF516_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8106998.1"
                     /translation="LFEAANNGTLLLDEVGEVSPAMQVKLLRVLQEREIRRVGENRSR
                     RVDVRIVAATNRDLAHGVFGGAFRQDLYYRLKVVELHVPPLRERRDDILPLARVLLAD
                     AALRMKRKVSGLVPNAADQLLRYGWPGNVRELENAMERAVALARGGRVELEDLPEEIR
                     RTPPETVPSGTVRPLDDIEKEYIVAALALNEGNQTRTAQQLHIGSATLYRKLKSYGLI
                     GGARTPSSGRRRDSV"
     gene            complement(726..992)
                     /locus_tag="VF516_04685"
     CDS             complement(726..992)
                     /locus_tag="VF516_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8106999.1"
                     /translation="MSISYGDKSVATLRSLVATIEQQFAVVPPPEPLRAAWAELVDVL
                     ALGPPPELRTCPTCGASGMRAATRCMHCWSSLAPLPASQAVGSS"
     gene            1259..1645
                     /locus_tag="VF516_04690"
     CDS             1259..1645
                     /locus_tag="VF516_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107000.1"
                     /translation="MTTSTIRDPSRETQADAVIEPPGARNAQLEPAALRASSEPDLES
                     RIRRRREELIGKLRELRSEASLEVVQARDRLQARLPELAHILKEGVVDGWASLGDAVK
                     HKLERWLAESERSLSSHGVPTKNGES"
BASE COUNT          287 a          586 c          573 g          256 t
ORIGIN      
        1 gctgttcgag gctgccaaca acgggacgct gctgctcgac gaggtcggcg aggtgtcgcc
       61 cgcgatgcag gtcaagctgc tgcgcgtgct ccaggagcgc gagatccggc gcgtcggcga
      121 gaaccggagc cggcgtgtcg acgtgcgcat cgtggctgcg acaaaccggg acctcgcaca
      181 tggcgtcttc ggtggtgcat tccggcagga tctttactat cggctcaagg tcgtcgagct
      241 ccatgtgccg ccgctccgcg agcgcagaga cgacatcctg ccgctcgccc gcgtgctgct
      301 cgccgacgcg gccctgcgga tgaagcgcaa ggtctcgggg ctggtcccga atgccgccga
      361 tcagctgctg cgctacggct ggccggggaa cgtgcgcgaa ctcgagaacg cgatggagcg
      421 ggcggtcgcg ctcgcgcgcg gaggccgcgt ggagctcgag gatctgccgg aggaaatccg
      481 ccgcacgcct cccgagaccg tccccagtgg gaccgtgcgc ccgctcgacg acatcgagaa
      541 ggagtacatc gtcgcggcgc tcgcgctcaa cgaaggcaac cagacccgca ccgcccagca
      601 gctccacatc gggtccgcga ccctgtaccg caagctgaag agctacggcc tgatcggagg
      661 cgcccggact ccgagcagcg gtcggcgtcg cgattctgtg tgacgtgtac cgacgtcgcc
      721 acggatcagc tgcttcctac cgcctggctc gccggcagcg gtgccagaga cgaccagcag
      781 tgcatgcacc gcgtcgctgc gcgcatgcca ctcgcgccac aggtcgggca ggtccgtagc
      841 tcggggggcg gcccgagagc gagcacgtcg acgagctcgg cccacgccgc acgcagcggt
      901 tcgggcggtg ggaccaccgc gaactgctgt tcgatcgtcg cgaccaggga acgcagcgtc
      961 gcgacggact tatctccata gctgatcgac atccagaact ccttgctcag ttcgccgggt
     1021 tactggtcgc ctgcagcttg catgcgacgg gccactgcgc ggtcacctgg ctcgttcgat
     1081 cgcgcgccct tccgggtgca actcctcgag cagcaatcct cggctgccaa gctcgccggc
     1141 gcgcgcgtcg ctctccttca aactgatgga cagttcttca ttgtgagcga gcaggagccc
     1201 gcgcggtgac caggagcgcg gcttgctcgc ggcttgctac ttgcagcgac gatggcgcat
     1261 gacgacgagc acgatcagag atccttctcg cgagacccag gccgacgcag tgatcgaacc
     1321 acccggcgcg cgcaacgccc agctcgagcc tgcagccctg cgcgccagct cggagcctga
     1381 tctcgagtcg cgcatcaggc ggcgccgcga ggagctgatc gggaagctcc gcgagctccg
     1441 gtcggaggcg agcctcgagg tggtccaggc ccgcgacaga ttgcaggcca ggctgcccga
     1501 gctcgcgcac atcctcaagg agggcgttgt cgacggctgg gcgagcctcg gtgacgccgt
     1561 gaagcacaag ctcgagcggt ggctcgccga atccgaacgt tcactctcca gccacggcgt
     1621 gcctaccaag aacggggaat catgatgatc cgtccactcc acgatcgtat cctcgcgcaa
     1681 cgcctcgacg agcaggaccg ga
//
LOCUS       DASVOR010000212         8126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_498982,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000212 DASVOR010000000
VERSION     DASVOR010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8126
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_498982"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF516_04695"
     CDS             <1..220
                     /locus_tag="VF516_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107001.1"
                     /translation="ESLRLDYDVPAIETVFVTPAGVAATGAGETSITLVPAAIGNAVF
                     DATGARLRDVPFTPARVLAALRAAGSAG"
     gene            complement(1056..1397)
                     /locus_tag="VF516_04700"
     CDS             complement(1056..1397)
                     /locus_tag="VF516_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107002.1"
                     /translation="MAMRSSNVTDCWVVAVPSSWAAVPSWLAHPAAASKPIKTKPSHD
                     FMTLLSFVGPAIEVIAKRVTVLWRIGILGESQFCMNIAILSTRVSVQDGPSCSWPAGH
                     ASSSSAAADRA"
     gene            1396..2805
                     /locus_tag="VF516_04705"
     CDS             1396..2805
                     /locus_tag="VF516_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107003.1"
                     /translation="MCSADDRVVAGVVSLDTCVGADLFLRETFNGNGRSCATCHPVAH
                     NFVIDPTFISKLPSTDPLFVAETNTALAHLEIPAVMRQFGLILENVDGFAPDPTTHFV
                     LRSVPHTLSLATSITNPPNSANPPANRTGWSGDGAPGSGALRDFQTGAITQHYTKSLS
                     RVVGTDFRLATDGELDRIDHFMRQLGRLNELDLTTIVMTDAGANAGRQRFLTVGCNGC
                     HGNAGANASFGGGGNRNFNTGVESARNSALAAFPHDGGFGATAQPDGSFGDGTFNAPP
                     LIEAADTGPFFHTAVTISGASAHNTDVARTIEEAVAFYDSPAFNNSPSGQAGHIDLTA
                     TEIDNIGRFLRVLNASFNIALATKKIQAVGILREAFPGISDIELQLDQLAAGDAQDAL
                     NVLSAVPNLNLTAQSKLQHFIQLAQNQFGGNKTGGTTTDGTGDPSLGVSNMLSDLSQA
                     LSSLGTNMNHTIGDSVLMF"
     gene            3245..4297
                     /locus_tag="VF516_04710"
     CDS             3245..4297
                     /locus_tag="VF516_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107004.1"
                     /translation="MTDLAFHPPPKVLFICGNRNHTTTMHAIARELPDCERWFTPYYC
                     DDGTLLDYLRRAHLLEFVALGHDFRRRCLAYLQQHELATDVGGKRGGYDLVVTCSDLI
                     VPSNVAGIPLVGVQEGMIDPKLFWWRVMELCPWLKLPRWAAGTACTGLSHAYDAYCVA
                     SDGYRRDFIERGAPPERLVVTGLPNFDDFAGYVRPGHWIEGCVLACTSDGRETFRRDD
                     RKRFIQWALEIAGDRPLVFKFHPNERMNRAVEEVRRWAPRARALTSGSGEVLAANCHT
                     LITEWSTLAYVGLALGKPTYSYRDLETHRAMLPDQNGRAAKNIAAVCRDVMVRPGVRG
                     ARGVAAHADPTLQGAA"
     gene            4294..5082
                     /locus_tag="VF516_04715"
     CDS             4294..5082
                     /locus_tag="VF516_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HEX8107005.1"
                     /translation="MKVTVVIQARTGSTRLPGKVLLRAAGATLLERMVERVCAARTPS
                     EVVIATTESAIDDPIVGIARRAGVPCVRGHATDCLDRHVAAARATSADAVVKIPSDCP
                     LIDPDTIDRVIGAFLAEDDVDYASNLHPPTWPDGFDVEIMTRGALETAWREATRPLDR
                     EHTTPFLWDNPERFTTLDVRWQTGLDYSQSHRLVLDYPEDYAVIRAIHDELWSAGRTF
                     PLSDILALLDARPALAAINAMHRGTSWIQNHLGELRTLAHGGMP"
     gene            5079..5924
                     /locus_tag="VF516_04720"
     CDS             5079..5924
                     /locus_tag="VF516_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEX8107006.1"
                     /translation="MSFTRPGLALKVREHVIRMTRGGGCFLGASLSCADLLVHLYTKV
                     LRVTPEALDDPARDFLLLSKGHDVPALYATLAELGFMPTARLARHLDPDDVLYWHPNR
                     GVPGVEFHSGSLGHLLSVGAGIATDAKLRGAPSRVFVILGDGECDEGSVWEAALVAAA
                     RGLDNLVAIVDRNSYQANVATESLIPLEPFAPKWEAFGWRATRINGHDFDQLDRAFAE
                     LPAQRGKPTVIIADTVRNKGLPSLEGKPERWFVKLSPGEVEQLIAELHGAAAVPLTSS
                     PQVVR"
     gene            5930..>8126
                     /locus_tag="VF516_04725"
     CDS             5930..>8126
                     /locus_tag="VF516_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8107007.1"
                     /translation="MVLQPYEAQLAQLAAADPAVVVVTAENRAAIRNLPAVLGERFID
                     VGVAEQTMIGLAAGLALRGRKPIVHALAAFLTMRAYEFIRTDVGIGRLPVKLVGGVPG
                     FLSDANGPTHQAIEDIALMRGIPGMKVFCPADEADLVASLPALLADPAPCYIRYTAAP
                     AVCPHPLPGQPHRIETVADGDDVAILSFGLLVGEALDARARLAADGISARVLSVRMPK
                     PLDEAAVLAAAADTRLVVTVEDHLQTGGLYSAVCELLVRAGTRAELLPIALAERWFKP
                     MLLPDLLAREGFTGAQIAARILERLDHPRGGNRVMRNPADPVITRSRSLWARAKQLIP
                     GGTQTLAKGPGQHVQGVAPIYLARGRGARVWDVDGNEYLDMTMAVGPLSLGYADPVID
                     EAIRTQLDDGITFSLMHPLEVEVAELIREVVPHAERVRFSKTGADATSAAIRVARAFT
                     GRERVVCCGYHGWHDWYIATTDRGAGIPTATRELVSTFDYNDLDSVRAAVDVDTACVI
                     FEPMVVEPPQSGFLEGVRAICDQHGALLVFDEMWTGFRIALGGAQQAFGVRADLATYS
                     KAIANGMPLSVLAGRADVMQKLERDVFFYTTFGGEALSLAAAAATIPELRRRQVPETL
                     AARGRVLRQGFARICAERGIGWTRCVGMDARTMVAFDAASLDPLLAKSFVQQELIRRG
                     VLWSGFHNLSWAHRDADLEYLLGCYHEILPALADHVAAGTLAGALRGTPV"
BASE COUNT         1248 a         2947 c         2706 g         1225 t
ORIGIN      
        1 cgagagcctg cgcctcgact acgacgtgcc cgcgatcgag accgtgttcg tgacgccggc
       61 aggcgtagcc gcgaccggcg caggggaaac atcgatcacg ctcgtcccgg cggcgatcgg
      121 caacgccgtg ttcgacgcga ccggtgcgcg gttgcgcgac gtaccgttca cgccggcgcg
      181 cgtgctggcc gccctgcgtg ccgcgggctc ggccggatag ccggttccga caggacccga
      241 acctgggaac ggacgcgcgc atgtcccacg gaccgtaagt cgcgacggcc accgcgacaa
      301 gtcagcacgc agcgcccggg acagcgtgta cagcgcgctc cactggtgac agaacgcgcg
      361 caggtagaag aacaaggtcc tgctcggttc cgacgatccg gtgctcggcc ccgagcgctg
      421 gatcgaggag ttcgagaggc agctgatcaa gcccgtacct ggtgagcgca atccacccgc
      481 gagtcactgg agcctgctcg aagctggatc aggaacggct cacgtcgcat gcgatcgaat
      541 gccagtgacc tattccgagg actccacaca gagtgcttcg gcgaggctct cgacaagctg
      601 catcacagct gctcgatcgc gcatcccccg gacttatatg cagcgctctg ccgtgaggta
      661 gcgcgtagac agctctctcg cactcgcgtg atgcctgcac acttgctgtg accacacctc
      721 tccgagacta gtcacgtcga cggttccgtg cccagatatt cccttcaccg tgacgtgggc
      781 ggctaagccg gacagctcta cctgcacacc gctcggccgc ccggcactgt gggcatcgca
      841 agaaaatcgc cagcctgcag attccccgac tgcctaacgc tgaatttcgc gaaaaactgt
      901 tgaggcattt taaccatccc tgtaaaactc tcgctagctg tagtgagttt cacggtggga
      961 ttgcgcagcg cgcggtctca cgagcgaggg aaatgaagtt gtttgttggt tggcctccgc
     1021 gggtcgatcc cgtccagata tcgacgctgc agtggtcatg cgcgatccgc cgcggcggac
     1081 gagctgcttg cgtggcccgc cggccagctg cagctggggc catcttgcac actgacacgt
     1141 gtcgataata tcgcaatatt catgcaaaat tgcgattcac cgagaatacc gattcgccaa
     1201 agaactgtga ctcgtttggc gatcacttcg atcgccggac caacgaagga gagtaaggtc
     1261 atgaaatcat gtgaaggctt tgtcttgatc ggtttgctag cggcggccgg atgcgccagc
     1321 caggaaggca ccgccgccca cgaggaaggc accgcgacca cccagcaatc ggtcacgttg
     1381 gacgaccgca tcgccatgtg ctcggctgat gatcgcgtcg tcgccggcgt cgtcagcctc
     1441 gacacctgcg tgggcgccga cctgttcctg cgcgagacgt tcaacggcaa cggcaggtcg
     1501 tgcgctacct gccacccggt ggcccacaac ttcgtgatcg atccgacgtt catctcgaag
     1561 ctgccgtcca ccgatccgct gttcgtcgcc gagacgaaca cggccctcgc ccatctcgag
     1621 attcccgcgg tgatgcggca gttcgggctg atcctggaga acgtcgatgg cttcgcgccg
     1681 gatccgacca cccacttcgt gctccgcagc gtgccgcaca cgctgtcgct cgccaccagc
     1741 atcacgaacc cgcccaattc ggcgaatccc ccggccaacc ggaccgggtg gtcaggtgat
     1801 ggtgcgccgg gcagcggtgc gctgcgagac ttccagaccg gcgcgatcac gcagcactac
     1861 accaagtcgc tgtcgcgcgt cgtgggcacg gacttccggc tggcgaccga cggcgagctc
     1921 gaccggatcg atcacttcat gcgccagctc ggccggctca atgagctcga cctcaccacc
     1981 atcgtgatga ccgacgcggg agccaacgcc gggcggcaga ggttcctcac cgtgggatgc
     2041 aacgggtgcc atggcaacgc cggcgccaac gccagcttcg gcggcggcgg caaccgcaac
     2101 ttcaacaccg gcgtcgagtc ggcgcgcaac tcggcgctcg cggcgttccc ccacgatggc
     2161 ggattcggcg ccactgccca gcccgacggt tcgttcggcg atggcacgtt caacgcgccg
     2221 ccgctgatcg aggccgcgga caccggcccg ttcttccaca ccgcggtgac gatcagcggt
     2281 gcgtcagccc acaacaccga tgtggcccgg acgatcgagg aggcggtcgc gttctatgac
     2341 tcgcccgcgt tcaacaactc gccgtctggc caggccgggc acatcgacct gacggccacc
     2401 gagatcgaca acatcggccg gttcctgcgc gttctgaacg cgtcgttcaa catcgcgctc
     2461 gccacgaaga agatccaagc tgtcgggatc ttgcgggagg ctttccccgg tatcagcgac
     2521 atcgagctcc agctggacca gctcgccgcg ggggatgccc aggatgcctt gaacgtcctc
     2581 tcggcggtcc cgaacctcaa cctgaccgcg cagagcaagc tgcagcactt catccagctt
     2641 gcgcagaatc agttcggcgg caacaagacc ggggggacga ccaccgacgg caccggcgac
     2701 cccagcctgg gcgtcagcaa catgctctcc gatctgtcgc aggcgctcag ctcgctcggc
     2761 accaacatga accacacgat cggcgatagt gtcctgatgt tctgagccgt cacgcgccgc
     2821 gtcgcgtgca cgtacgacgc ggaggtggtg atcgagccac tgctggatca ccagctccgc
     2881 ccctcacacc agtccgatga acggcctgtg gatcccaccg gcccggccgc cgcggcgtcc
     2941 ggggcgcgcg acggcaggtg accggaatcg gacaccgcgg ctcgatccag tgtcgattct
     3001 cgaacacgcg gcagcaagcg gcggacggcg gtatcagacg tttttcgcag caacgtgagg
     3061 gcgcggtcgc gcgtcctagc gttcacatga cagcacatct cgcccgttcg cgacgctgcc
     3121 gtcgagagcc atcgcagagt catcatcgcg acaccgatct gcgatcggga gcggcgcgat
     3181 tcgcttccac cacgtcacga tcgcgtggca gacgcgtcgc gatctgcgat caggctggtg
     3241 cggcgtgaca gacctagcgt tccacccacc gccgaaggtg ctgttcatct gcggcaatcg
     3301 caatcacacc accacgatgc acgcgatcgc gcgggagctg cccgactgcg agcgctggtt
     3361 cacaccgtat tactgcgatg acggcacgct gctcgattac ctgcggcgcg cccacctgct
     3421 cgagttcgtc gcgctcggcc acgacttccg ccggcgctgc ctggcctacc tgcagcagca
     3481 cgagctcgcg actgatgtcg gcggcaagcg cggcggctac gacctcgtcg tcacctgctc
     3541 ggatctgatc gtgccgtcga acgtcgccgg catcccgctg gtcggggtgc aggagggcat
     3601 gatcgatccc aagctgttct ggtggcgggt gatggagctg tgcccgtggc tcaagctgcc
     3661 gcgctgggcg gccggcacgg cgtgcaccgg gctgtcgcac gcctacgacg cgtactgcgt
     3721 ggcgagcgac ggctaccgcc gcgacttcat cgagcgcggc gcgccacccg agcgactggt
     3781 ggtcaccgga ctgcccaact tcgacgactt cgccggctac gtccgccccg ggcactggat
     3841 cgagggctgc gtgctggcgt gcacctcgga cggccgcgag acgttccggc gcgacgatcg
     3901 caagcggttc atccagtggg cactcgagat cgcgggcgac cggccgctgg tgttcaagtt
     3961 ccaccccaac gagcggatga accgcgcggt cgaggaggtc cggcgctggg cgccccgcgc
     4021 gcgggcgctg accagcggct cgggcgaggt gctggcggcg aactgtcaca cgctgatcac
     4081 cgagtggtcg acgctggcgt acgtcgggct cgcgctcggc aagccgacct acagctaccg
     4141 cgacctcgag acccatcgcg cgatgctgcc cgaccagaac ggccgcgccg ccaagaacat
     4201 cgccgcggtg tgccgcgacg tgatggtgcg gccgggtgtg cgcggcgcgc gcggcgtggc
     4261 ggcgcacgcg gatccgacgc tgcagggagc ggcatgaagg tcaccgtcgt gatccaggcc
     4321 cggaccggct cgacccggct gcccggcaag gtcctgctcc gcgccgccgg cgcgacgctg
     4381 ctcgagcgca tggtcgagcg ggtgtgcgcc gcgcggacgc cgagcgaggt ggtgatcgcg
     4441 accaccgagt cggccatcga tgacccgatc gtcgggatcg cccggcgcgc cggcgtcccc
     4501 tgcgtccgcg gccacgcgac cgactgcctc gatcgccacg tcgcagcggc gcgcgcgacc
     4561 tcggccgacg ccgtggtcaa gatcccgtcg gactgcccgc tgatcgatcc ggacaccatc
     4621 gaccgcgtga tcggcgcgtt cctcgccgag gacgacgtcg actacgcgtc caacctgcac
     4681 ccgccgacct ggcccgacgg gttcgacgtc gagatcatga cccgcggcgc gctcgagacc
     4741 gcgtggcgcg aggcgacccg gccgctcgac cgcgagcaca ccacgccgtt cctgtgggac
     4801 aaccccgagc ggttcaccac cctcgacgtg cggtggcaga ccgggctcga ctacagccag
     4861 tcgcaccgcc tggtgctcga ctatccggag gactacgcgg tgatccgcgc gatccacgac
     4921 gagctgtggt ccgccggccg gacgttcccg ctgagcgaca tcctggcgct gctcgacgcc
     4981 cgccccgcgc tcgccgcgat caacgcgatg caccgcggga ccagctggat ccagaaccac
     5041 ctcggcgagc tgaggacgct cgcccacgga ggcatgccat gagcttcacc cgacccggcc
     5101 tcgcgctgaa ggtccgcgag cacgtgatcc ggatgacccg cggcggcggg tgcttcctcg
     5161 gcgcgtcgct gtcgtgcgcc gatctgctgg tccacctgta caccaaggtg ctgcgggtga
     5221 ccccggaggc gctcgacgat ccggcgcgcg acttcctgct cctgtccaag ggccacgacg
     5281 tgcccgcgct gtacgccacg ctggccgagc tcgggttcat gccgaccgcg cggctggccc
     5341 gccacctcga tcccgacgac gtgctgtact ggcaccccaa ccgcggcgtg ccgggcgtcg
     5401 agttccactc cggctcgctc ggccacctcc tgtcggtcgg cgccgggatc gcgaccgacg
     5461 ccaagctgcg cggcgcgccg agccgcgtgt tcgtgatcct cggcgacggc gagtgcgacg
     5521 agggctcggt gtgggaggcc gcgctggtcg ccgccgcccg cgggctcgac aacctggtcg
     5581 cgatcgtcga ccgcaacagc taccaggcca acgtcgcgac cgagtcgctg atcccgctcg
     5641 agccgttcgc gcccaagtgg gaggcgttcg gctggcgggc gacgcggatc aacggccacg
     5701 acttcgacca gctcgaccgc gcgttcgccg agctgcccgc ccagcgcggc aagccgaccg
     5761 tgatcatcgc cgacaccgtg cgcaacaagg gcctgccgtc gctcgagggc aagcccgagc
     5821 gctggttcgt caagctgtcg cccggtgagg tcgagcagct gatcgccgag ctccacggcg
     5881 ccgccgccgt cccgctgacc tccagcccgc aggtggtgcg atgacgtcca tggtccttca
     5941 gccctacgag gcccagctcg cccagctcgc cgccgccgac cccgccgtcg tggtggtcac
     6001 cgcggagaac cgcgccgcga tccgcaacct gccggcggtg ctcggcgagc ggttcatcga
     6061 cgtcggcgtc gccgagcaga ccatgatcgg cctcgccgcc gggctcgcgc tgcgcggccg
     6121 gaagccgatc gtccacgcgc tcgccgcgtt cctcaccatg cgggcctacg agttcatccg
     6181 caccgacgtc ggcatcggcc ggctgccggt caagctggtc ggcggcgtgc cgggcttcct
     6241 gtccgacgcc aacgggccga cccaccaggc gatcgaggac atcgcgctga tgcgcggcat
     6301 cccgggcatg aaggtcttct gcccggccga cgaggccgac ctggtcgcgt cgttgcccgc
     6361 gctgctcgcc gatccggcgc cgtgctacat ccgctacacc gcggcgccgg cggtgtgccc
     6421 gcacccgctg cccggccagc cccaccggat cgagaccgtc gccgacggcg acgacgtggc
     6481 gatcctgagc ttcggcctgc tggtcggcga ggcgctcgac gcccgcgccc ggctcgccgc
     6541 cgacggcatc tcggcgcgcg tgctcagcgt ccggatgccc aagccgctcg acgaggcggc
     6601 ggtgctggcc gcggccgccg acacccggct ggtggtcacc gtcgaggacc acctgcagac
     6661 cggcgggctg tactcggcgg tgtgcgagct cctggtccgc gccgggacgc gcgccgagct
     6721 gctgccgatc gcgctggccg agcgctggtt caagccgatg ctgctgcccg acctgctcgc
     6781 gcgcgagggc ttcaccggcg cgcagatcgc ggcgcggatc ctcgagcggc tcgaccatcc
     6841 gcgcggcggc aaccgggtca tgcgcaaccc cgccgatccg gtgatcacgc gctcgaggtc
     6901 gctgtgggcg cgtgccaagc agctgatccc cggcggcacc cagaccctcg ccaagggccc
     6961 cggccagcac gtccagggcg tcgcgccgat ctacctggcg cgcggccgcg gcgcgcgggt
     7021 gtgggacgtc gacggcaacg agtacctcga catgaccatg gcggtgggcc ccttgtcgct
     7081 cggctacgcc gacccggtga tcgacgaggc gatccggacc cagctcgacg acggcatcac
     7141 gttctcgctg atgcacccgc tcgaggtcga ggtcgccgag ctgatccgcg aggtcgttcc
     7201 ccatgcggag cgcgtgcggt tctccaagac cggcgccgac gcgacgtccg cggcgatccg
     7261 ggtcgcgcgc gcgttcaccg ggcgcgagcg cgtggtgtgc tgcggctacc acggctggca
     7321 cgactggtac atcgcgacca ccgaccgcgg cgccgggatc ccgaccgcga cccgcgagct
     7381 ggtatcgacc ttcgactaca acgacctcga cagcgtgcgc gcggcggtcg acgtcgacac
     7441 cgcgtgcgtg atcttcgagc cgatggtggt cgagccgccg cagtccggct tcctcgaggg
     7501 cgtgcgcgcg atctgcgacc agcacggcgc gctgctggtg ttcgacgaga tgtggacggg
     7561 ctttaggatc gcgctcggcg gcgcgcagca ggcgttcggc gtccgcgccg acctcgcgac
     7621 ctactccaag gcgatcgcca acggcatgcc gctgtcggtg ctcgccggcc gcgccgacgt
     7681 gatgcagaag ctcgagcgcg acgtgttctt ctacaccacg ttcggcggcg aggcgctgtc
     7741 gctcgccgcc gccgcggcga cgatccccga gctgcgccgg cgccaggtgc ccgagaccct
     7801 cgcggcccgc ggccgcgtgc tgcgccaggg cttcgcgcgg atctgcgccg agcgcggcat
     7861 cggctggacc cggtgcgtcg ggatggacgc ccgcacgatg gtcgcgttcg acgcggcgtc
     7921 gctcgatccg ctgctcgcca agtcgttcgt ccagcaggag ctgatccggc gcggcgtgct
     7981 gtggagcggg ttccacaacc tgtcgtgggc gcaccgcgac gccgacctcg agtacctgct
     8041 cggctgctac cacgagatcc tccccgcgct cgccgaccac gtcgccgccg gcacgctcgc
     8101 cggcgcgctg cgcggcacgc cggttg
//
LOCUS       DASVOR010000213        10608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_235776,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000213 DASVOR010000000
VERSION     DASVOR010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10608
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235776"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VF516_04730"
     CDS             complement(<1..1717)
                     /locus_tag="VF516_04730"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HEX8107008.1"
                     /translation="MVIEKSQLQPGAMGESVNHLHQRLALLGFEVPADERQDDRYGSG
                     TEAAVRRFQAANGLPVVNGRVDEDTARGLGLGGYPTSVAGVVCRPDGTPLPSVAVRLF
                     QHSAGGEKVVAESRSDAGGKFSLPWPQGISGGLSIRAEGSAARTLSWKAALSAGAIWV
                     RLSVGGEYRGATRFAVLTRALAPLVEAGAFHAVGGAGRAAELTALAEAARVPGPDVSR
                     YILSHKLAAKTGLDPCVCFALLTHGVTPETLASLATTAGDVEALADAHVDQVLDAALA
                     QHRDELRAGLAAAVADNLVSDLDVDAAVHQLHALRVEHFAHKPITWASPQLCSEEHTC
                     CAVASAPVTAGAGRNPTEDLMDAARPLVVHHVPLRDVIATSLPDAAAQRQVLEAFATH
                     GNHARFGTVVDAAAGLSPDQRSHLRFTLEAAALLGNHLPLVSYVQGLRAAKVIATVGD
                     LARFDEADWADFLHQADPGGSKIALPADAGASAGDRIALFARALARRLEARYPTTALA
                     GRLAKAAGSLPLARSGAVQQFLARTPSFCVRHTHVDRFFHEMGHEMGHEMGPEMGHEM
                     GHEMGS"
     gene            complement(2146..2484)
                     /locus_tag="VF516_04735"
     CDS             complement(2146..2484)
                     /locus_tag="VF516_04735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107009.1"
                     /translation="MPLLPAAQEYASPPVTVNVGEPTAPTAPIALTSALTAGGSAPGS
                     AFGAQAVMAIAEHESAVAIKYLFLMTIDLLVQVNIERGVARNQHPPPRDRPRHNKSLD
                     RMRGQAITSS"
     gene            2438..4492
                     /locus_tag="VF516_04740"
     CDS             2438..4492
                     /locus_tag="VF516_04740"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107010.1"
                     /translation="MTGGLAYSCAAGSSGIRCWGSDTNGQLGDGSTGQRIQPVAVTGI
                     QFGTAMVSAGGLHTCAVVNGDARCWGANNFGQLGNGQPGNDTTGVPDSHVPVDVTNLS
                     TGAQAIAAGDSHACAVVNGAARCWGLNSFGQLGDGSVTLRRSPVAVSNLGSGVQAITA
                     GGAHSCAIVNGAAMCWGLNNSGQLGNATPGNPSPGDSHAPVPVTGLGSGVIAIAAGQN
                     HTCAIVTGGTVKCWGSNSSGQLGNSATGGISGSPVAVTGLDSGVIAIAAGQNHTCAIA
                     NGGAAVCWGSNAGGQLGDGSPDPNIPLPGDSNAPVQVTGLNSGVQSIAAGAQHSCATT
                     TAGGVFCWGLNSTAQIAVDPDTASLFGAPRQISGLVLGGCADGSDEQVFNNGMVGCAG
                     AVAFASRGTLCAAGYRPATSLQWTTNRGAGIPAHHYWTNDPLLYGGSGPSACFVSLSV
                     GTNCGADTPMRVCAANNTDPEGNFCNWTQCGLYANAPNQYFGGCFSNTTAGTLCIPNI
                     DPPSGCSQFNSCPKWENGFFTSSSTEGTGIAGPWVSCTQFCADAGQCNPASCDTMWAP
                     VDSCNSQATTRLGQLQGNVASLVGSEANFDSPQALQQQSTAAQGALRVTSSFHVETAN
                     FHQTCSASRPLGGFIVGGGGGGGGGGGGCQLFWNQRYVCDLNLTNVNPTTAPNDACGC
                     AQ"
     gene            complement(4547..5938)
                     /locus_tag="VF516_04745"
     CDS             complement(4547..5938)
                     /locus_tag="VF516_04745"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEX8107011.1"
                     /translation="MIFTRLPGHALAAVLGAAWLSACSLDVPDLNNPGIDTLQVHPDA
                     VSIGAACTGLLIGNRSQIANEFGYVDELGIVGRESYNFDAADPRFVNELLAGPLSQAS
                     PLGGNFWQVPYANIRLANVILDALDKVPAGDLDDSGKAGVRGFVHTMIALELLQVIVT
                     RDTIGAVIDTDRDLSQPLGQIVSKDMVYARIASLLDSALPDLDAAGKAFPFLLSSGYA
                     GFDTPAAFRKFNRALRARAAAYTKDYAAVNDALSLSFLDDSAKADFTVGVYYTYSTKT
                     GDTTNGLINPNIYVHPSVQADAQKKADGKTLDDRYTNKVTTTDNPGSVTGTPLASSLV
                     FTPLYGSPSARVGLIRNEELILLKAEQLFFTGDTAKAIDELNIVRTRSGGLEPLSPTT
                     DPTTFTSELLYERRYSLLFEWGHRWIDVRRFDRATDLPLDDPTYVRNMRFPIPLQECN
                     ARPNEPACKLGSL"
     gene            complement(5950..9408)
                     /locus_tag="VF516_04750"
     CDS             complement(5950..9408)
                     /locus_tag="VF516_04750"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX8107012.1"
                     /translation="MLSPLAAQAQNAQPAKGAPAAKGRDVTGTVVDVDGNPVAGATVA
                     VAGGGPTTTAAADGSFKLTGVATTNVMVEITADGFTAKQVPVLGAATPLQLQVVIVRP
                     APAAPPPVETRRLGGVVGDAGHAPIAGATVTVHGTSIQAVTAADGSFALPGAPLGEVT
                     LDVAAPNQPPTSVAVPGDKPIVAITAGASAQPAAPARRKITGKVIEPATKEPIAAAQV
                     EVVGTDAVVFTGADGTFELDNLPPGPVKLDITAPEHESHVLEVGPDQSTVDVPLALSK
                     GEQIVIEGRAPVIVKTNLANGASVIDDKDLNRVSAQTLDAAMTAKLSGSNIQSNSGAP
                     GGGAQLRLRGISTINGQSTPLYVIDGVVISNIAVSNGSNAVTAAAAGGNPSAQDNPVN
                     RLADLNPNDIENIEVLKGASAAALYGSKAANGVVVITTKRGRNGENHAEVTQRFGFSQ
                     VSKELGARTFTSIDDVPKRLQAAYMAAGGKTFDHEAEIEQTALAMETLGSASGGTENG
                     NYYGSLLIADDPGVVKGTFYQKQTGRIALGYKFGDRVRFGVTANLIHSLTDRGLTNND
                     NTGTSNWVVLSSTPNFVDLRPVNGIYPSNPGVGAHTNPLQTVALFQNREDVWRLIAGS
                     TISIDAYRSPDGNSTVKLLGNFGADDFTQKNNINSPNQLIFEPSDGLPGTLVDGTTKN
                     LNWNTGAGAVWSYRPEDKAFRSVLSGGLTYESVDLNSVYVIAANLSAGQTNVNAATSV
                     NTNENNLRTKDTGLYAQEEVAVLDDRLSFLGGLLAERSSLNGDTNKYYLFPKAAAVYS
                     FIGKGDALSAMFDTLRGRIAYGEAGNRPTYNNKFTPLVVSNNIDGNAGIVILGNFGDA
                     KIEPERQREIELGVDMATKDQRVVAEVTAYQRNISNLILQRALPFSTGFTTEFSNGGG
                     LRNRGLEAAITVRPLPPKTAVDWTSRGTLTLNRSMVTSTPDHLPYDINAAGFGTGLGS
                     FRIEEGHSATEIVGQVDPAGTVQHLGDGEPSFRVGWSNLVTAGDFTFSALLDWQHGSN
                     VINLTRLLYDASGNSPDVAAADARITAFGVNKDIRPYIEDASFVKLREVSVAYTLPRR
                     LIGQLAPLRSLQVSVSGRNLLTFTGYSGLDPEVSNFGNQPIGRNYDVAPYPPSRSYWL
                     SVTAGI"
     gene            9987..>10608
                     /locus_tag="VF516_04755"
     CDS             9987..>10608
                     /locus_tag="VF516_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8107013.1"
                     /translation="MTTASSERARVAIANRRDGGVVFWLVRMYAFAALVLVAGVVFSA
                     LAVYSYFSLHAPPVPDLRIYARVTPAVSRMYAADGTMLGEFAKEWREIVPFERMPQRL
                     IDAFLAVEDHDFYHHGGLYWKGIGRAVWANLTAGDFAQGGSTITQQVAKQFLGGEKSL
                     SRKGKEAIMARRLEARYSKNAILAVYMNHIYLGAGAWGVAAAARRYF"
BASE COUNT         1567 a         3613 c         3756 g         1672 t
ORIGIN      
        1 cgctgcccat ctcgtgcccc atctcgtgcc ccatctcggg ccccatctcg tgccccatct
       61 cgtgccccat ctcgtggaag aagcggtcga cgtgggtgtg ccgcacgcag aacgacggtg
      121 tgcgcgcgag gaactgctgc acggcgccgg accgggccag cggcagcgag cccgcggcct
      181 tggcgagccg gccggccagc gcggtggtcg ggtagcgggc ctcgaggcgc ctggccagcg
      241 cccgcgcgaa cagcgcgatg cgatcgccgg cggaggcgcc ggcgtccgcc ggaagcgcga
      301 tcttcgagcc gcccggatcc gcctggtgca agaagtccgc ccagtccgcc tcgtcaaacc
      361 gcgccagatc gccgacggtc gcgatcacct tggcggcgcg caggccctgg acgtaactca
      421 cgagcggcag gtggttcccg agcagcgccg cggcctcgag cgtgaagcgg agatggctgc
      481 gctggtccgg ggacagtccg gcggcggcat cgaccacggt cccgaaccgg gcatggttgc
      541 cgtgggtggc gaacgcctcg agcacctggc gctgggcggc cgcatcgggc agcgaggtcg
      601 cgatgacgtc gcgcagcggc acgtggtgga cgaccagcgg ccgggccgcg tccatcagat
      661 cctcggtggg gttgcggccg gcgccggcgg tcaccggcgc cgacgcgacc gcgcagcacg
      721 tatgctcctc ggagcacagc tgcggagagg cccacgtgat cggcttgtgc gcgaagtgct
      781 cgacgcgcag cgcgtgcagc tggtggaccg cggcgtcgac atcgaggtcg gagaccaggt
      841 tgtctgcgac cgccgccgcc aggcctgcgc gcagctcatc gcgatgctgc gccagcgccg
      901 catccagcac ctggtcgacg tgggcgtcgg cgagcgcctc cacgtcgccc gcggtggtgg
      961 cgagcgacgc cagcgtctcg ggggtcacgc cgtgggtcag cagcgcgaag cacacgcagg
     1021 gatcgagccc ggtcttcgcg gccagcttgt gcgagagaat gtagcgcgag acgtcgggtc
     1081 ccgggacgcg ggccgcctcg gcgagcgccg tcagctcggc ggcccgcccg gcgccgccga
     1141 cggcatggaa ggcgccggct tcgaccagcg gtgccagcgc ccgggtcagc accgcgaacc
     1201 gcgtcgcgcc gcggtactcg ccgccgacgc tcaggcggac ccagatcgcc ccggcggaca
     1261 gcgccgcctt ccaggacagc gtcctcgcgg cgctgccctc ggcgcggatg ctcagcccgc
     1321 cgctgatgcc ctgcggccac ggcaaggaga acttgccgcc ggcatccgac ctggactcgg
     1381 ccacgacctt ctcgccaccg gcgctgtgct ggaacagccg cacggcgacg ctggggagcg
     1441 gtgtcccgtc gggccggcac acgacgcccg cgaccgacgt ggggtagccg ccgaggccga
     1501 gcccgcgcgc ggtgtcttcg tccacccgcc cgttcaccac cggcagcccg ttcgccgcct
     1561 ggaaccgccg gaccgcggcc tcggtgccgc tgccgtagcg atcgtcctgc cgctcgtcgg
     1621 ccgggacttc gaaccccagc aacgcgagtc gctggtgaag atgattgacg gactcgccca
     1681 tcgcaccggg ctgcaactgc gacttctcaa tgaccatgtt tacctctcga gcctgttcgc
     1741 ggagggggca aagatccgcg gtgacgtctc gtacgtccga cgaacgcctg gcgctgcctc
     1801 gagcgagagc caccagcgcc gaccgaccgc ggcactcact tcactaccac cacaatgata
     1861 gattccctgg atactccgcg cacgcgacac tgtcaagcag cgctgaagaa gttcggtgtc
     1921 cattgcgcag tcgactcgcg atctccacgc gacaacgtcg cgggacagcc gtcgcagctc
     1981 acgtgcaccg tctcatatcg gtacaagcag aacaccgcgg aacacgctgt atcggggccg
     2041 cagaacgtcg cagaacgtcg cagaacgcct gtatcgaacc ggcggaacac cctgatacag
     2101 cggaacagtc ctcgtcgaga accggcaaac tgcggaaccg gcaagtcagc tcgacgtgat
     2161 tgcttgaccg cgcatgcggt cgaggctctt attgtgccgt ggacgatcgc gcggaggggg
     2221 atgctgattt ctggcaacgc cgcgctcgat gttcacttgc accaggaggt cgattgtcat
     2281 gaggaatagg tatttgatcg ccacggcact ctcgtgttcc gcgatcgcca tcacggcttg
     2341 tgcgccgaac gcggatcccg gagctgaccc gccggccgtg agcgccgagg tcagcgcgat
     2401 cggcgcggtc ggcgcggtcg gctcgccgac gttgaccgtg accggcgggc tcgcgtattc
     2461 ctgcgcggcc ggcagcagcg gcatccggtg ctggggttcc gacaccaacg gacagctcgg
     2521 cgacggctcc accggtcagc gcatccagcc cgtcgcggtc acgggcatcc agttcgggac
     2581 cgccatggtc tccgccggcg gcctgcacac ctgcgcggtc gtcaacggcg atgccaggtg
     2641 ctggggcgcc aacaactttg gccagctcgg caacggccag cccggcaacg acacgaccgg
     2701 cgtccccgat agccacgtgc cggtcgacgt caccaatcta tcgaccggcg cgcaggcgat
     2761 cgccgcgggc gactcgcacg cgtgcgcggt ggtcaacggt gccgcccggt gctggggcct
     2821 caacagcttt ggccagctcg gcgacggctc ggtgacgctc cgccgctcgc cggtcgcggt
     2881 cagcaacctg ggcagcggcg tccaggcgat caccgccggt ggcgcgcaca gctgcgcgat
     2941 cgtcaacggc gctgccatgt gctggggcct caacaacagc ggccagctcg gcaacgccac
     3001 gccgggaaat ccttcgcccg gagacagcca cgcaccggtc ccggtcaccg gcctcggcag
     3061 cggcgtgatc gcgatcgccg ccggtcagaa ccacacctgc gcgatcgtga ccggcggtac
     3121 ggtgaagtgc tgggggagca actcgtccgg gcagctcggg aacagcgcga ccggcgggat
     3181 ctccggttcc ccggtcgcgg tcaccggcct cgacagcggc gtgatcgcga tcgccgccgg
     3241 ccagaaccac acctgcgcga tcgcgaacgg cggtgcggcg gtgtgctggg gctccaacgc
     3301 cggcggccag ctcggcgacg gctcgccgga cccgaacatc cctctgccgg gggacagcaa
     3361 cgcgccggtc caggtcaccg gcctgaacag cggtgtccag agcatcgccg cgggcgctca
     3421 gcacagctgc gcgacgacga ccgccggcgg cgtgttctgc tggggcctca acagcaccgc
     3481 ccagatcgcg gtcgaccccg acacggcgtc cctgttcggc gcgccgcgcc agatctccgg
     3541 cctggtcctg ggcggctgcg cggacggcag cgacgagcag gtgttcaaca atggcatggt
     3601 cggatgcgcc ggcgcggtgg cgttcgccag ccgcggcacg ctgtgcgcgg ccgggtaccg
     3661 accggcgacc agcttgcagt ggaccaccaa ccggggcgcc gggatcccgg ctcaccacta
     3721 ctggacgaac gacccgctgc tgtacggcgg atccggtccg tcggcgtgct tcgtgtcgct
     3781 gtcggtcggc accaactgtg gggccgacac gccgatgcgc gtgtgcgccg cgaacaacac
     3841 cgatccggag ggcaacttct gcaactggac gcaatgcggg ctgtatgcga acgctccgaa
     3901 ccagtacttc gggggatgct tcagcaacac gaccgccggc acgctgtgta tcccgaacat
     3961 cgatcccccg agcggatgct cgcagttcaa ctcgtgcccg aagtgggaga acgggttctt
     4021 cacgtcgtcg agcacggagg gcaccgggat cgccggtcca tgggtgagct gcacgcagtt
     4081 ctgtgcggac gccggccagt gcaatccggc gtcttgcgat acgatgtggg cgcccgtcga
     4141 ttcgtgcaac agccaggcga ccacgcgcct cggccagctc caggggaacg tggccagcct
     4201 cgtcgggagt gaggccaact tcgacagccc gcaggccctg cagcagcaga gtacggccgc
     4261 gcagggagcg ctccgcgtga cgtcgagctt tcacgtggag acggccaatt tccaccagac
     4321 ttgcagcgcc agccgtccgc tggggggatt catcgtgggc ggcggcggcg gcggcggcgg
     4381 cggcggcggc ggatgtcagc tgttctggaa ccagcgctac gtgtgcgacc tcaatctcac
     4441 gaacgtcaat ccgaccaccg cccccaacga cgcgtgcggc tgcgcgcaat gaccgcgccc
     4501 cggccggctg ccgcgccggc cggcaccgca gcacgcgacc gggggctcag aggctgccca
     4561 gcttgcacgc cggctcgttc ggccgcgcgt tgcactcctg gagcgggatc gggaaccgca
     4621 tgttgcggac atacgtcgga tcgtcgagcg gcaggtcggt ggcccggtcg aaccggcgga
     4681 cgtcgatcca gcgatggccc cattcgaaca gcagcgagta gcgccgctcg tagagcagct
     4741 cgctggtgaa cgtcgtgggg tccgtggtcg gcgagagcgg ttccagcccg cccgagcggg
     4801 tccggacgat gttgagctcg tcgatcgcct tcgcggtgtc cccggtgaag aacagctgtt
     4861 cggccttgag caggatcagc tcctcgttgc ggatcaaccc cacccgcgcc gacggactgc
     4921 catacagcgg ggtgaacacc agcgacgagg ccagcggcgt gccggtcacg ctgcccgggt
     4981 tgtcggtcgt cgtcaccttg ttcgtgtatc gatcatcgag tgtcttcccg tcggccttct
     5041 tctgcgcgtc ggcctgcacc gacgggtgga cgtagatgtt cgggttgatc agcccgttgg
     5101 tggtatcgcc ggtcttggtc gagtacgtgt agtacacgcc gacggtgaag tccgccttcg
     5161 cggagtcgtc gaggaacgac agcgagagcg cgtcgttcac cgccgcgtag tccttggtgt
     5221 acgccgccgc ccgcgcgcgg agcgcgcggt tgaacttgcg gaacgccgcc ggcgtgtcga
     5281 acccggcgta tcccgacgac agcaggaacg ggaacgcctt gcccgccgcg tcgagatcgg
     5341 ggagcgcgct gtcgagcagg ctcgcgatcc tggcgtagac catgtccttg ctgacgatct
     5401 ggccgagcgg ctgcgagagg tcgcggtcgg tgtcgatcac cgctccgatg gtgtcgcggg
     5461 tcacgatgac ctggagcagc tccagcgcga tcatcgtgtg gacgaagccg cgaacccccg
     5521 ccttgccgct gtcgtccaga tcacccgccg ggaccttgtc gagcgcgtcc aggatcacgt
     5581 tggcgagccg gatgttggcg tacggcacct gccagaagtt gccgccgagc gggctggcct
     5641 ggctgagcgg gccggcgagc agctcgttga cgaaccgtgg atcggcggcg tcgaagttgt
     5701 aggactcgcg gccgacgatc ccgagctcgt cgacgtagcc gaactcgttc gcgatctggc
     5761 tgcggttgcc gatcagaagg ccggtgcagg cagcgccgat cgacaccgcg tcggggtgga
     5821 cctgaagcgt gtcgatcccc gggttgttga ggtcggggac gtcgaggctg cacgccgaga
     5881 gccaggcggc gccgagcacg gcggcgagcg catgaccggg aagtcgtgtg aagatcatgg
     5941 acggtctcct cagatccccg ccgtgaccga cagccagtag ctgcggctgg gcggataggg
     6001 tgcgacgtcg tagttgcggc cgatcggctg gttgccgaag ttcgagacct cgggatccag
     6061 gccgctgtag ccggtgaacg tcagcaggtt gcggccgctg accgagacct gcagcgaccg
     6121 cagcggcgcg agctggccga tcagccgcct gggcagcgtg taggccaccg agacctcgcg
     6181 cagcttcacg aacgacgcgt cctcgatgta cggccggatg tccttgttga ccccgaacgc
     6241 cgtgatccgc gcatccgcgg cggcgacgtc cggcgagttt ccggaggcgt cgtacagcag
     6301 gcgggtcagg ttgatcacgt tcgagccgtg ctgccagtcg agcagcgcgc tgaacgtgaa
     6361 gtcaccggcg gtgaccaggt tcgaccagcc gacgcggaac gacggctcgc cgtcgccgag
     6421 gtgctggacc gtgccggccg ggtcgacctg tccgacgatc tcggtcgcgc tgtgaccctc
     6481 ctcgatccgg aacgagccca gcccggtgcc gaaccctgcc gcgttgatgt cgtacggcag
     6541 gtggtcgggc gtgctggtga ccatcgagcg gttcagcgtc agcgtgccgc ggctcgtcca
     6601 gtcgaccgcc gtcttgggcg gcagcggccg gaccgtgatc gcggcctcga ggccgcggtt
     6661 gcgcagcccg ccgccgttgg agaactcggt ggtgaacccg gtcgaaaacg gcagcgcgcg
     6721 ctgcaggatc aggttcgaga tgttgcgctg gtacgcggtg acctcggcga ccacgcgctg
     6781 gtccttggtc gccatgtcga cgccgagctc gatctcgcgc tgccgctcgg gctcgatctt
     6841 ggcgtcgccg aagttgccga ggatcacgat gccggcgttg ccgtcgatgt tgttgctcac
     6901 gaccagcggg gtgaacttgt tgttgtaggt cggccggttg ccggcctcgc cgtacgcgat
     6961 gcggccgcgc agcgtgtcga acatcgccga cagcgcgtcg cccttgccga tgaacgagta
     7021 caccgcggcg gccttgggga acaggtagta cttgttggtg tcgccgttga ggctcgagcg
     7081 ctcggcgagc aggccgccga ggaacgacag ccggtcgtcg agcacggcga cctcctcctg
     7141 cgcgtagagc ccggtgtcct tggtccgcag gttgttctcg ttggtgttga ccgaggtcgc
     7201 cgcgttgacg ttggtctggc cggcgctgag gttggccgcg atcacgtaga ccgagttgag
     7261 gtcgacgctc tcgtaggtca ggccgccgga cagcacgctg cggaacgcct tgtcctcggg
     7321 cctgtacgac cacacggcgc cggcgccggt gttccagttc aggttcttgg tcgtgccgtc
     7381 gaccagcgtg ccgggcaggc cgtcggacgg ctcgaagatc agctggttgg gcgagttgat
     7441 gttgttcttc tgcgtgaagt cgtcggcgcc gaagttgccg agcagcttga cggtgctgtt
     7501 gccgtccggg ctgcggtagg catcgatcga gatcgtcgac ccggcgatca gccgccacac
     7561 gtcctcccgg ttctggaaca gcgcgacggt ctgcagcggg ttggtgtggg cgccgacccc
     7621 tggattggac ggatagatcc cgttgaccgg ccgcaggtcg acgaagttcg gcgtgctcga
     7681 cagcacgacc cagttcgagg tcccggtgtt gtcgttgttg gtcagcccgc gatcggtcag
     7741 cgagtggatc aggttggcgg tgacgccgaa ccggacccga tcgccgaact tgtagccgag
     7801 cgcgatccgc ccggtctgct tctggtagaa cgtgcccttc acgacgccgg ggtcgtcggc
     7861 gatcagcagc gagccgtagt agttgccgtt ctcggtcccg cccgacgcgc tgcccagggt
     7921 ctccatcgcg agcgcggtct gctcgatctc ggcctcgtga tcgaacgtct tgccgccggc
     7981 ggccatgtac gccgcctgga ggcgcttcgg cacgtcgtcg atcgacgtga acgtccgcgc
     8041 gccgagctcc ttggacacct gcgagaaccc gaaccgctgg gtcacctcgg cgtggttctc
     8101 gccgttgcgc ccgcgcttgg tcgtgatcac cacgacgccg ttggcggcct tggagccgta
     8161 cagcgcggcg gccgatgcgc ccttgagcac ctcgatgttc tcgatgtcgt tggggttgag
     8221 gtcggcgagc cggttgaccg ggttgtcctg ggccgacggg ttgccgccgg ccgccgccgc
     8281 ggtgaccgcg ttcgatccgt tcgacaccgc gatgttcgag atcaccacac catcgatcac
     8341 gtagagcggc gtcgactgac cgttgatcgt cgagatgccg cgcagccgga gctgggcgcc
     8401 gccgcccggc gcccccgagt tggactggat gttcgagccc gagagcttgg cggtcatcgc
     8461 ggcgtcgagc gtctgggcgc tgacccggtt gaggtccttg tcgtcgatca ccgacgcgcc
     8521 gttggcgagg ttggtcttga cgatcaccgg cgcgcggccc tcgatcacga tctgctcgcc
     8581 cttggacagc gcgagcggca cgtcgacggt gctctggtcg gggccgacct ccagcacgtg
     8641 gctctcgtgc tcgggcgcgg tgatgtcgag cttgaccggg ccgggcggca ggttgtcgag
     8701 ctcgaacgtg ccgtcggcgc cggtgaacac cacggcgtcg gtgccgacca cctcgacctg
     8761 cgcggcggcg atcggctcct tggtcgccgg ctcgatcacc ttgccggtga tcttgcgcct
     8821 ggccggcgcg gcgggctgcg cggacgcgcc cgcggtgatc gcgacgatcg gcttgtcgcc
     8881 ggggacggcg acgctggtcg gcggctggtt cggagcggcg acgtcgagcg tgacctcgcc
     8941 gagcggggcg ccgggcagcg cgaagctacc gtcggccgcg gtcaccgcct ggatcgaggt
     9001 gccgtgcacg gtcaccgtcg cgccggcgat cggggcgtgg ccggcgtcgc cgaccacgcc
     9061 gccgagcctg cgggtctcga cgggcggcgg cgcggcgggc gccggcctca cgatcacgac
     9121 ctggagctgg agcggcgtcg ccgcaccgag caccgggacc tgcttcgccg tgaagccgtc
     9181 ggcggtgatc tcgaccatca cgttggtcgt cgcgacgccg gtcagcttga agctgccgtc
     9241 ggccgcggcg gtggtcgtcg gaccgccgcc ggcgaccgcc accgtcgcgc cggccaccgg
     9301 gttgccgtcg acatcgacca ccgttccggt gacgtcgcgg cccttggcag ccggtgcgcc
     9361 cttcgcgggc tgtgcgttct gcgcctgggc ggcgagcgga gacagcaaga atgcaagtac
     9421 gcacgttgct gctcccaacg gatgtgcaaa ccatctcgta ggcatgaggt cctcctgaag
     9481 gcaaaatcgc tcggtgaagc cggggcaatc taagcgcttg tgaccgaatc ttcacaagaa
     9541 aatgccctga ccttggcttg gccagaccgc ctggattgcg gcccaggcag tgtcgcagcc
     9601 cataaatttg gttagttata ttactctact tgcacgtttg taagcagccc tgcgaacgcg
     9661 gcacaacgcc cagaaacagg acgaggtgcc gtgtcctcga cgcaccacga gcgctcttgt
     9721 tccggatatg gagcgactcg tggtcgagga agctcgaccg agcggtgccg gcgcggggcc
     9781 ggtctgcaga aggccggtgc cggcggtcga accgctcggg cgaagacccc tcatccgggc
     9841 gcgggagcac gccccggcga gccctcccga gcatcaccgc acatcgcggc ggtgccgagc
     9901 gcacgcgccg acatccgtcg acagcgtagg acttgcccct ccgcggcgcg ccatggctag
     9961 atcacgatcg agtgcacgaa ttcgctgtga ccacggccag ctccgaacga gcgcgggtcg
    10021 cgatcgcgaa ccgccgcgat ggcggcgtcg tgttctggct cgtgcggatg tacgcgttcg
    10081 ccgcgctggt gctcgtcgcc ggtgtcgtgt tctccgcgct cgcggtctac agctacttct
    10141 cgctgcacgc gccccccgtc ccggacctcc ggatctacgc gcgggtcacg ccggcggtgt
    10201 cgcggatgta cgcggccgac ggcacgatgc tcggcgagtt cgccaaggag tggcgcgaga
    10261 tcgtgccgtt cgagcgcatg ccgcagcggc tgatcgacgc gttcctcgcg gtcgaggacc
    10321 acgacttcta ccaccatggc gggctgtact ggaaaggcat cggccgcgcg gtgtgggcca
    10381 acctcaccgc gggcgatttc gcccagggtg gctcgacgat cacccagcag gtcgccaagc
    10441 agttcctcgg cggcgagaag tcgctgtcgc gcaagggcaa ggaagcgatc atggcgcgcc
    10501 ggctcgaggc ccggtactcc aagaacgcga tcctcgcggt ctacatgaac cacatctacc
    10561 tcggcgccgg cgcgtggggc gtggcggccg cggcgcgccg ctacttcc
//
LOCUS       DASVOR010000214         2960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410601,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000214 DASVOR010000000
VERSION     DASVOR010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410601"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF516_04760"
     CDS             <1..404
                     /locus_tag="VF516_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107014.1"
                     /translation="TREEARASLEGLTLAELRQVHSRLLAEQPATPFNQGGLTTRNKA
                     DAITQVVELAVGRNLDSQAIGRAVGGRPEVTSLERARKELESLQGRLGLAEDRRRKSG
                     ARVASLAESRLRGQISDKRREISRLESEGKA"
     gene            518..2596
                     /locus_tag="VF516_04765"
     CDS             518..2596
                     /locus_tag="VF516_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107015.1"
                     /translation="MLNPSMVDIARGNVVRDPGRDAARAAAMAQSAGQASGAVRTPAA
                     ERRRLSQERAARLDRDRSAGEALAHLVEHIENTDGQPDRMDLIRERGDALQAQGLPAE
                     VARAVATAHTSQEALSVLRTAAAGHHIELIGKPGRSGGGFDRARHAPVGGDITPGEQV
                     TVIRPEVVWTDPKTGERLQIARAVVVPSGGTLDNSRVPLQASSMTETTSHIGPVPKVT
                     GYVKGREIRPGMVINAADLLPMHEKQGATTGENPNPRGHQRWIRVGMIGNSNTPGFKE
                     YEGQGTLTGGMYIVVDQNGRAVGRMSANTIAETSTDVPIEPKAQINQVRMPIKIKVRN
                     KVTGEDEIRDSVTVVGVPSKHAQEELGLPEPQFLREGVLDPKDPNYGLNGSRPKGIKQ
                     PFGPRVEGARSREEAAADRTRRREIYASGEEERARRLAEEKAAKKAEAAAEEAKRREE
                     SDRKMADYREEQRRKFLRTRHQAMTEIIAAGGREEHDWETRQDTFNTDVNGLIRKELK
                     RNRGAINDLARAYGVKWRGKSEDEKIDAVIAAVRAGKTPNEEIDAPQVKAPVSETAKA
                     SAAEQRRNAGIKKNFERLRDLLVKGDHREARFHLDFSMSDNATAQLAESFGLQGLSGS
                     ALREALFEAIREGQTPNIDLGALELLSTDELKARAKRLVDVPLSGRSRQELIDLIRGN
                     TPRGRMPGGA"
     gene            2644..>2960
                     /locus_tag="VF516_04770"
     CDS             2644..>2960
                     /locus_tag="VF516_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107016.1"
                     /translation="MVGDLRAGLAALETVVSAVYIGAAGDTRHKLPEAAQATLRDALT
                     QALEDLERRDYRLTRAALLGGARTAMAGGAADLDTRVDFRLPLDVRDAIAGTTRNIRA
                     HLA"
BASE COUNT          534 a         1049 c         1050 g          327 t
ORIGIN      
        1 acacccgcga ggaggcccgc gcgagcctgg aagggctgac cctcgcggag ctgcgccagg
       61 tgcactcccg gctgctcgcc gaacagccgg ccaccccgtt caatcagggc ggcctgacca
      121 cccgcaacaa ggccgacgcg atcacgcagg tggtcgagct ggccgtcggg cgcaacctcg
      181 attctcaggc gatcgggcgc gccgtcggtg gtcggcccga ggtcacttcc ctggagcggg
      241 cgcgaaagga gctggagtcg ctgcaaggcc ggctcgggct cgccgaggac aggcggcgca
      301 agagcggcgc ccgggtggcc agcctcgcgg agagcaggct ccggggccag atctccgaca
      361 agcggcgcga gatcagccgc ctggagagcg agggaaaagc gtaggcccgc agggcgggca
      421 gccatcgagc caggccggcg ggctccagcg gacggccacg aaggtcggct ccgacctgcg
      481 caccgtcggc tggaaggacg ccaggacggg ccggccggtg ctcaacccgt ccatggtgga
      541 catcgcccgg ggcaacgtgg tccgcgaccc cgggcgcgac gccgcgcggg ccgccgccat
      601 ggcgcagtct gccggccagg cttccggcgc tgtccggaca ccggcggccg agcggcgtcg
      661 actcagccag gagcgggcgg cgcgcctcga ccgcgaccgg tccgccggtg aagcgctcgc
      721 ccacctggtc gagcacatcg agaacaccga cgggcagccc gaccggatgg acctgatccg
      781 cgagcggggc gacgccctcc aggcacaggg actgcccgcc gaggtggccc gagctgtcgc
      841 tacggcacac acctcacaag aggcgttgag tgtcctgcgc accgccgccg ccggccatca
      901 cattgagctg atcggtaagc cgggacgctc gggcggaggc ttcgaccgcg cccgccatgc
      961 cccggttggt ggagacatca cccccggcga gcaggttacg gtcattcgcc ccgaggtggt
     1021 atggacggac ccgaagaccg gcgagcggct ccagatcgcc cgagcggtgg tcgtcccttc
     1081 cgggggcaca cttgacaact cgcgtgttcc cttgcaagct agttccatga ccgagacaac
     1141 ttcccacatc ggccccgtac cgaaggtcac cggctacgtg aagggccggg agatccgacc
     1201 cggaatggtg atcaacgcgg ccgatctgct ccccatgcac gagaagcaag gggcgaccac
     1261 tggcgagaac ccgaacccgc gcggccatca gcggtggatt cgggtgggca tgatcggcaa
     1321 ctcgaacacg cccggtttca aggagtacga ggggcaaggc acgctcaccg gcggcatgta
     1381 cattgtcgtc gaccagaacg gccgcgctgt cggccggatg tccgccaaca ccatcgccga
     1441 gaccagcacc gacgtgccca tcgagcccaa ggcgcagatc aaccaggtgc ggatgccgat
     1501 caagatcaag gttcgtaaca aggtcaccgg cgaggacgag atccgcgaca gcgtcaccgt
     1561 cgtcggcgtg ccgtcgaagc acgcgcagga ggagttgggg ctcccggagc cgcaattcct
     1621 gcgcgagggt gtcctggacc cgaaagaccc gaactacggc ctcaacggca gccgccccaa
     1681 gggcatcaag cagccgttcg gcccgcgcgt cgaaggtgcc cgcagccgcg aggaggccgc
     1741 cgccgaccgc acccgccgcc gcgagatcta cgcctcgggc gaggaggagc gggcgcgccg
     1801 gctcgccgag gagaaggccg ccaagaaggc cgaggccgcc gccgaggaag cgaagcggcg
     1861 cgaagaatcg gaccgcaaga tggccgacta tcgcgaggag cagcgccgca agttcctccg
     1921 gactcgacac caggccatga cggagatcat cgccgcaggt gggcgcgagg agcacgactg
     1981 ggagacccgc caggacacct tcaacaccga cgtcaacggc ctgatccgca aggagctgaa
     2041 gcgcaaccgg ggcgccatca acgacctggc acgcgcctac ggcgtgaagt ggcgcggcaa
     2101 gtccgaggac gagaagatcg acgcggtgat tgccgccgtg cgggccggca agactccgaa
     2161 cgaggagatc gacgcccccc aggtcaaggc gccggtcagc gagaccgcca aggcgagcgc
     2221 tgccgagcag cgccgcaacg cggggatcaa gaagaacttc gagcggctcc gggatctgct
     2281 cgtcaagggg gaccaccgcg aggcccggtt ccacctcgac ttctccatgt ccgacaacgc
     2341 gaccgcccag ctcgccgagt cgttcggcct ccaggggctg tccggcagtg cgctgcgcga
     2401 ggcgctgttc gaggcgatcc gcgagggtca gaccccgaac atcgacctgg gcgcccttga
     2461 gctgctcagc acggatgagc tgaaggccag ggcgaagcgg ctggttgacg tgccactgtc
     2521 cggcaggagc cggcaggagc tgatcgacct catccggggg aacaccccgc gcggccggat
     2581 gcccggcggg gcgtagtgga tgagcgcgag cttgccgacc acgaacgccg cgtacgggcg
     2641 tccgtggtcg gcgacctgcg cgccggcctc gccgcgctcg aaacggtcgt cagcgccgtc
     2701 tacatcggcg cggccgggga cacccgccac aagctgcccg aggcggcgca ggcgaccctg
     2761 cgcgacgccc tcacacaggc cctggaggac ctggagcgcc gcgactaccg gctgacccgg
     2821 gccgcgctgc tcggcggcgc acgcaccgcc atggccggcg gcgcggccga cctggacacc
     2881 cgggtcgact tccggctacc cctcgacgtg cgcgacgcga tcgcgggcac cacccgcaac
     2941 atccgggcgc acctcgccga
//
LOCUS       DASVOR010000215         2983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_206721,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000215 DASVOR010000000
VERSION     DASVOR010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206721"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..970
                     /gene="pqqB"
                     /locus_tag="VF516_04775"
     CDS             <1..970
                     /gene="pqqB"
                     /locus_tag="VF516_04775"
                     /inference="COORDINATES: protein motif:HMM:NF003523.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqB"
                     /protein_id="HEX8107017.1"
                     /translation="LAVAATYRPRRRRRRRASLYVRVLGSAAGGGSPQWNCGCANCTG
                     VRAGHASLRARTQASAAVSADGRQWFLLNASPDVRAQIEAFPALHPRRLRDTPIGGIA
                     LNNGDVDACLGLFILREDQPLVLYASEAVERGLREHNALTRTLERRPGQLIWNRLRAG
                     EVVPLVGAAGPSGLTIEAVPVPGTVPLHLKGLAAPSPEDNLAFVLRDEHGRSAMYAPT
                     AASLTPLLDERARAADVLLFDGTFFRDDELTSLGLTTRSARDMAHWPLGGPEGSLRWL
                     AQLPCRRKILTHINNTNPILREGAPERDELIAAGVEPAYDGLEVEL"
     gene            967..1674
                     /gene="pqqC"
                     /locus_tag="VF516_04780"
     CDS             967..1674
                     /gene="pqqC"
                     /locus_tag="VF516_04780"
                     /EC_number="1.3.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline-quinone synthase PqqC"
                     /protein_id="HEX8107018.1"
                     /translation="MTASPASEASFLDRLRTEGARRYHDEHPFHRRMHDGALARADLQ
                     RWVANRYYYQTRIPIKDALILAKSEDPGFRRMWIQRIHDHDGTTADEGGLVRWQRLGE
                     AVGLERGALIACASVLPGVRFACDAYVHFVRDASLVEAVASSLTEFFAPDLMVKRIAA
                     WEKHYAWVDRAGLDYFRSRVTQGRRDSEQAIAFVIEHARTAELQDRCVAALVRKTEIL
                     WHLLDCVQSAGAGPGAP"
     gene            1674..1928
                     /locus_tag="VF516_04785"
     CDS             1674..1928
                     /locus_tag="VF516_04785"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HEX8107019.1"
                     /translation="MPAALSADSRPQLARKARLRTDRLTGETLLLYPEHGLVLNASAA
                     AILRLCDGRPAGDIATELGAPLADVLEFLATLSERGLVHT"
     gene            1925..>2983
                     /gene="pqqE"
                     /locus_tag="VF516_04790"
     CDS             1925..>2983
                     /gene="pqqE"
                     /locus_tag="VF516_04790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018149 - peptide cross-linking [Evidence
                     IEA];
                     GO_process: GO:0018189 - pyrroloquinoline quinone
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone biosynthesis protein
                     PqqE"
                     /protein_id="HEX8107020.1"
                     /translation="MSPNPRPYVLVAELTYRCPLRCAYCSNPTQVDDRPALATADWLR
                     VLDEASELGVVQVHFTGGEPLLRDDLEPLVARAAQLELYSNLITSGLPLTRERLAGLK
                     AAGLCAVQLSFQAARPGPSAAIAGIDAFEAKRRVAAWVRELDLPLTVNVVLHRGNLDE
                     VDRIIALAEELGAERLELANTQYLGWAHQNRDALMPSLAQLDAARVVVAAARDRLRGR
                     MELLFVLPDYHGDRPRTCMNGWGERYIVVTPDGRAMPCHAAHILPLAFPDLRTQSLVT
                     AWRDSEAVQRYRGTDYLREPCRSCERREIDHGGCRCQAFLLTGDATATDPVCELSPRH
                     DLVVAARAAAAEPAPPLTL"
BASE COUNT          382 a         1139 c         1071 g          391 t
ORIGIN      
        1 tttggcggtc gcggcaacgt accgcccgcg ccggcgaaga agaaggcgcg ctagcttgta
       61 cgtgcgcgtg ctcggctcgg cggccggcgg tggctcgccg cagtggaact gcggctgcgc
      121 caactgcacg ggtgtgcgcg ccgggcacgc gtcgctccgc gcgcgcaccc aggcctcggc
      181 ggccgtcagc gccgacggcc ggcagtggtt cctgctcaac gcctcacccg acgtgcgcgc
      241 gcagatcgag gcgtttcccg ccctgcaccc gcgccgcctg cgcgacacgc cgatcggcgg
      301 gatcgcgctc aacaacggcg acgtcgacgc gtgcctcggc ctgttcatcc tgcgcgagga
      361 ccagccgctc gtgctctacg cgagcgaggc ggtcgagcgc gggctgcgcg agcacaacgc
      421 gctcacccgc acgctggagc gccggcccgg acagctgatc tggaaccggc tgcgcgccgg
      481 cgaggtcgtg ccgctggtcg gcgcggccgg gccgagcggg ctgacgatcg aggccgtgcc
      541 cgtgccgggc accgtgccgc tgcacctcaa gggcctcgcg gcgccgtcgc ccgaggacaa
      601 cctcgcgttc gtgctgcgcg acgagcacgg ccgcagcgcg atgtacgcgc cgaccgccgc
      661 gtcgctcacc ccgctgctcg acgaacgcgc gcgcgcggcc gacgtgctgc tgttcgacgg
      721 cacgttcttc cgcgacgacg agctgacctc gctcggcctg accacgcgca gcgcgcgcga
      781 catggcgcac tggccgctcg gcgggcccga gggcagcctg cgctggctgg cccagctgcc
      841 gtgccggcgc aagatcctga cccacatcaa caacaccaac ccgatcctgc gcgagggcgc
      901 gcccgagcgc gacgagctga tcgccgcggg ggtcgagccg gcgtacgacg ggctggaggt
      961 cgagctgtga ccgcttcgcc ggcgtccgag gccagcttcc tggaccggct gcgcaccgag
     1021 ggcgcgcggc gctaccacga cgagcacccg ttccaccgcc gcatgcacga cggcgcgctg
     1081 gcccgcgccg atctgcagcg ctgggtcgcg aaccgctact actaccagac gcggattccg
     1141 atcaaggacg cgctgatcct cgccaagtcc gaggatcccg ggtttcgccg gatgtggatc
     1201 cagcggatcc acgaccacga cggcaccacc gccgacgagg gcggcctggt ccgctggcag
     1261 cggctgggcg aagcggtcgg cctggagcgc ggcgcgctga tcgcgtgcgc cagcgtgctg
     1321 ccgggcgtgc ggttcgcgtg cgacgcctac gtccacttcg tgcgcgacgc gtcgctggtc
     1381 gaggcggtgg cgtcgtcgct gaccgagttc ttcgcgcccg atctcatggt caagcgcatc
     1441 gccgcgtggg agaagcacta cgcgtgggtc gaccgcgccg gcctggacta cttccgcagc
     1501 cgggtcaccc agggccggcg cgactccgag caggcgatcg cgttcgtgat cgagcacgcg
     1561 cgcaccgccg agctgcagga ccgctgcgtg gccgcgctgg tgcgcaagac cgagatcctg
     1621 tggcacctgc tcgactgcgt gcagagcgcc ggcgcgggtc caggagcgcc gtgatgcccg
     1681 cggcgctgtc cgcggacagc cggccgcagc tcgcccgcaa ggcgcgcctg cgcaccgatc
     1741 ggctgaccgg cgagaccctc ctgctctacc ccgagcacgg cctggtgctc aacgcctcgg
     1801 cggccgcgat cctgcggctg tgcgacgggc ggcccgcggg cgacatcgcc accgagctcg
     1861 gcgcgccgct cgccgacgtg ctcgagttcc tggcgacgct gagcgagcgc ggtctggtgc
     1921 acacgtgagc ccgaacccgc gcccgtacgt cctggtcgcc gagctgacct accgctgtcc
     1981 gctgcgctgc gcctactgct cgaacccgac ccaggtcgac gaccgcccgg cgctcgccac
     2041 cgccgactgg ctgcgcgtgc tcgacgaggc cagcgagctc ggcgtggtgc aggtccactt
     2101 caccggcggc gagccgctgc tgcgcgacga cctcgagccg ctggtcgcac gcgccgcgca
     2161 gctcgagctg tactcgaacc tgatcaccag cggcctgccg ctcacccgcg agcgcctggc
     2221 cgggctgaag gcggccgggc tgtgcgcggt ccagctgtcg ttccaggccg cgcggcccgg
     2281 cccgtccgcc gcgatcgccg ggatcgatgc gttcgaggcc aagcggcggg tcgccgcgtg
     2341 ggtccgcgag ctcgacctgc cgctgaccgt gaacgtcgtg ctgcaccggg gaaacctcga
     2401 cgaggtcgat cggatcatcg cgctcgccga ggagctgggc gccgagcggc tcgagctcgc
     2461 caacacgcag tacctcgggt gggcccacca gaaccgcgac gcgctgatgc ccagcctggc
     2521 ccagctcgac gccgcgcgtg tggtggtcgc cgcggcgcgc gatcgcctgc gcggccgcat
     2581 ggagctgttg ttcgtgctgc ccgactacca cggcgaccgg ccgcgcacgt gcatgaacgg
     2641 ctggggcgag cgctacatcg tggtcacgcc cgacggacgc gccatgccgt gccacgccgc
     2701 gcacatcctg ccgctcgcgt tccccgacct gcgcacgcaa tcgctggtga ccgcgtggcg
     2761 cgactcggag gcggtccagc gctaccgcgg caccgattac ctgcgcgagc cctgccggtc
     2821 gtgcgagcgg cgcgagatcg accacggcgg ctgccgctgc caggcgttcc tgttgaccgg
     2881 cgacgcgacc gccaccgatc cggtgtgcga gctgtcaccg cgccacgatc tggtcgtcgc
     2941 ggcgcgcgcg gccgcggccg agccggcccc gccgctgacc ctg
//
LOCUS       DASVOR010000216         2479 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148218,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000216 DASVOR010000000
VERSION     DASVOR010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148218"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VF516_04795"
     CDS             complement(<1..756)
                     /locus_tag="VF516_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107021.1"
                     /translation="MNHLYLIGALLAGAAFGCGDNTGGFTPWELEDLHGGSGFSLRVP
                     LFEVPAGHESQNCYFVKVPDINGNGQDVWIDRVLTAINPGSHHVNVFRVKTVIGLDPA
                     AGSPTMLGDYPATVIVGADDYQHSPCWGSANWADWPLIANSQHANAGELETDWKLPEN
                     VAIRLTPGEMLMLQTHYVNSTDQPTLYGAKVGINFYQRQQATPPVEMGSLFATQQNIR
                     ICASQPNVTFSGTCRFPNAVTVAAANGHFHKRGK"
     gene            complement(753..1736)
                     /locus_tag="VF516_04800"
     CDS             complement(753..1736)
                     /locus_tag="VF516_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107022.1"
                     /translation="MLSRCLLPLAFLVACGGGEQTPSWQTLSSGEPAALLAVWGSSAR
                     DVWVVGGRGELMGSPTILHFDGKAWSRVDSGQRGLDLWWVFGFAGGDVFFTGAGGTIL
                     RYRNTRFERMQTPRSGTLFGLWGAAPDDLWAVGTGDDSKGIVWRYDGSQWSDVTPPDA
                     PNLVLKVHGQRTDDVWMSCASGVTLHWDGAALTSVATGVTSSLFSVVTTPEVAIAVGG
                     YTGQGAIVEHTAARAGWTVQPSLAPVTWRGVAAGASRVFVVGESGVVGQRTSAGWTLT
                     DRFVTPLTFHAAWVDPAGGLWGVGGMFDTLPLTSDGFLAYYGVGAPPEISP"
     gene            complement(2005..>2479)
                     /locus_tag="VF516_04805"
     CDS             complement(2005..>2479)
                     /locus_tag="VF516_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107023.1"
                     /translation="DELPRRLLVSMADHVAFGMMPKNQPLDPPVRDAIVDLLIDTLWT
                     APSERSEARRYYLGRARGLPAHQVDNELHEIDRIAGASSDVTWGALERGIWSDQSTVT
                     PGFLALSGLEALRACSHAVQARGGSLESCLSQATAPSVWSRWPPAVRAAPARRRP"
BASE COUNT          402 a          861 c          844 g          372 t
ORIGIN      
        1 cttgccgcgc ttgtggaagt gaccgttggc ggccgcgacc gtcaccgcgt tggggaaccg
       61 gcacgtcccc gagaaggtca cgttcggctg gctggcgcag atgcggatgt tctgctgggt
      121 cgcgaacagc gagcccatct cgaccggcgg ggtggcctgc tggcgctggt agaagttgat
      181 cccgaccttg gcgccgtaca gcgtgggctg gtcggtgctg ttcacgtagt gcgtctgcag
      241 catcagcatc tcgccgggcg tgaggcgaat ggcgacgttc tcgggcagct tccagtcggt
      301 ctcgagctcg ccggcgttgg cgtgctgcga gttggcgatg agcggccagt cggcccagtt
      361 cgcgctgccc cagcacgggc tgtgctggta gtcgtcggca ccgacgatca ccgtggccgg
      421 gtagtcgccc agcatggtcg gcgagcccgc ggcgggatcg aggccgatga ccgtcttgac
      481 ccggaacacg ttgacgtggt ggctgccggg gttgatcgcg gtcaagaccc gatcgatcca
      541 gacgtcctgg ccattgccat tgatatcagg caccttcacg aagtagcagt tctggctttc
      601 gtggcctgcc gggacctcga acagcggcac ccgcagggaa aaaccgctgc ctccgtggag
      661 gtcctccagc tcccagggag tgaatccgcc cgtgttgtca ccgcagccga acgcggcacc
      721 cgccagcaac gcaccgatca ggtaaaggtg attcatggcg agatctccgg aggcgctccg
      781 acgccatagt aggccaggaa tccgtcggat gttagcggca acgtatcaaa cattccaccg
      841 actccccaca gccctcccgc cggatccacc caggccgcgt gaaaggtgag tggggtcacg
      901 aaccgatcgg tcagtgtcca tcctgccgag gtccgctggc cgacgacgcc ggactctccg
      961 accacgaata cccggctggc tcccgccgcg actccgcgcc acgtgaccgg tgccagcgac
     1021 ggctgaacgg tccatccagc acgtgcggcg gtgtgctcga cgatcgcccc ctgaccggtg
     1081 tagccgccga ccgcgatcgc gacctccggt gtggtcacga ccgagaacag cgaggacgtg
     1141 accccggtcg ccacgctggt gagcgcggcg ccgtcccagt gcagcgtcac cccgctggcg
     1201 cacgacatcc agacgtcgtc ggtgcgctgg ccgtggacct tcaagaccag gttcggagca
     1261 tcgggcggcg tcacgtcgct ccactgcgac ccgtcgtagc gccacacgat gcccttgctg
     1321 tcgtcgcccg tgccgaccgc ccagagatca tcgggtgcgg cgccccacag gccaaagagg
     1381 gtgccgctgc gcggcgtctg catgcgctcg aagcgggtgt tgcgatagcg gaggatcgtc
     1441 ccgccggcgc cggtgaagaa cacatcgcca cctgcgaatc cgaacaccca ccacagatcc
     1501 aggccgcgct ggccgctgtc gacgcgactc caggccttgc cgtcgaaatg aagaatcgtc
     1561 ggtgatccca tgagctcgcc ccgaccgccg accacccaga cgtcccgcgc gctcgagccc
     1621 cagaccgcga gcagcgcggc cggctccccg gacgacagcg tctgccagct cggcgtctgc
     1681 tcgcctccac cgcacgccac gaggaacgcg agcggtagca gacagcgcga cagcatcact
     1741 gaagggtaac gcaagccagg gggccggggc gggtgctgca tgtctccctg cgtggcagtc
     1801 gaagacgtac cagccgtgcg caagcctgtc caggacggct ccgccggcct cgctaaaatg
     1861 tgatcacgtc gcagactccg cgccacgtgg gtcgctcccc ctgccggcaa acgttgcagc
     1921 gagatcgatc acggcccggc gatcgcgcgc gaacgtcgag ggcagatcaa tgattccggt
     1981 ggcttggatc gctgcgccgg acggtcatgg tcgacggcga gccggcgcag cccggaccgc
     2041 gggcggccac cgcgaccaca cgctcggcgc ggtcgcctgc gagaggcagc tctcgagcga
     2101 gccgccccgc gcctgtaccg catgtgagca ggcccggagc gcctcgaggc cggacagcgc
     2161 caggaagccg ggcgtgaccg tcgactgatc ggaccagatg ccgcgctcga gcgcgcccca
     2221 ggtgacgtcc gacgaggccc cggcgatccg atcgatctcg tggagctcgt tgtcgacctg
     2281 gtgggcgggc aggccgcgcg cgcgcccgag gtagtagcgt ctcgcttcgc tgcgctccga
     2341 cggcgccgtc cacagcgtgt cgatcaggag gtccacgatc gcgtcgcgca ccggcggatc
     2401 gaggggctgg ttcttgggca tcatgccgaa cgcgacgtgg tcggccatgc tgaccaggag
     2461 ccggcgcggc agctcgtcg
//
LOCUS       DASVOR010000217         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265026,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000217 DASVOR010000000
VERSION     DASVOR010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265026"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            376..1095
                     /locus_tag="VF516_04810"
     CDS             376..1095
                     /locus_tag="VF516_04810"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8107024.1"
                     /translation="MTAAAPAVASAPASAAYGFHAPRIARVLDRLHRDARRDMFRLAL
                     RLPLLVPDMLGRHKADPARQAHHMRDIYIPVSREQGQFLYLMARSIGAQRIVEFGTSF
                     GISTLYLAAAVRDNGGGAVIGSELEPSKHDRASEHLREAGLDDIASVRLGDAQATLAD
                     VAAPIDLVLLDGWKDLYLPILHLLTPKLRAGALVLADNIYTFKRALRPYVEYVQSGQY
                     GFVSTTLDLADGFELSVYTGP"
     gene            1416..>1785
                     /locus_tag="VF516_04815"
     CDS             1416..>1785
                     /locus_tag="VF516_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107025.1"
                     /translation="MFAALDPEGFLRWRAREGDPCLVRLPFVGEGLVTGSPEGARELF
                     TADPAIFMSLPANPFEDLLGPGSLLLKSGAAHTRERKLLLPAFHGERMRAYAEVIEDA
                     TAAQVARLQPGETFVVQEVAR"
BASE COUNT          249 a          642 c          590 g          304 t
ORIGIN      
        1 cggcaccctg ccggccagct ggaccgtcca gacgttctgg cagaccgccg gcagcggcgt
       61 gttccccggc gaccagaaca agttcaacgg cgggatcatc cggctctttg cgttcgccct
      121 cggattgccg ttgccggtgg cgtgcggagc ggatgggatg cctgactgat tgcccagttg
      181 ccgatctcgt gtcgatgggg ctcgggatgc tgccaggcgg gacccggctc aggcgacaac
      241 ttccctgaga catggggggc ctcgggccgc gactgggcag tcgccggtgc ggtccccatg
      301 ctcgccaagc cgcgtaatca gggtggtttc aaaccgatcg gtgctctcgt agtcaatcgc
      361 tcctagactt acgccatgac cgccgccgcc cctgccgttg ccagcgcccc cgcatccgcc
      421 gcctacggct ttcatgcccc gcggatcgcc cgcgtcctcg accgccttca tcgcgacgcc
      481 cgccgcgaca tgttccgcct cgcgctccgg ttgccgctcc tcgtccccga catgctcggc
      541 cggcacaagg cggatccggc gcgtcaggcc caccacatgc gcgacatcta catcccggtg
      601 tcgcgcgagc agggccagtt cctgtatctc atggcccgca gcattggggc ccagcgcatc
      661 gtcgagttcg gcacctcgtt cggcatctcg accctctacc tcgccgccgc cgtccgcgac
      721 aacggcggcg gcgccgtgat cggcagcgag ctcgagccat ccaagcacga ccgcgccagc
      781 gagcatctgc gcgaggccgg gctcgacgac atcgccagcg tccgcctggg cgacgcccag
      841 gcgaccttgg ccgatgtcgc cgcccccatc gacctcgtcc tgctcgacgg ctggaaggac
      901 ctctacctgc ccatcctcca cctgctcacg ccgaagctac gggcgggcgc gctggtcctg
      961 gccgacaaca tctacacctt caagcgcgcg ctccggccct acgtcgagta cgtgcagtcg
     1021 ggccagtacg gcttcgtgtc gaccacgctc gatctcgcgg acggcttcga gctatcggtc
     1081 tacactggcc cctagacgcg cgcggtcact ggcacatgcc ggcctgggcg ccgattgtct
     1141 ccggcgccag cacgaccgtg ttgccgttgg cgacgtacgg gatgtgcggc cggatctgga
     1201 ccgaggtgtt ccactggcgc gaggtagtcg tacatccaga atcggccctc gtccggatcc
     1261 gcttgtatgc cggtccggga cctcgcgcta cttcacgccg cggcagatca ggaaccggtg
     1321 gggaacttct cggtcacgtc gctcggaagc atagcggggc gggatcacga gggtatggtt
     1381 cgcgtatcgt gagatggcct agagtcgtgc agggggtgtt tgccgccctg gatcccgaag
     1441 gattcctccg ctggcgcgcg cgcgagggcg atccctgcct ggtgcgcctg cccttcgtgg
     1501 gcgagggcct ggtgaccggc agccccgagg gcgcgcgcga gctcttcacc gcggatcccg
     1561 cgatcttcat gagcctgccg gcgaaccctt tcgaggactt gctcggcccg ggatcgcttc
     1621 tcctgaagtc cggcgcggcg cacacgcgcg agcgcaaact gctcttgccc gccttccacg
     1681 gcgagcgcat gcgggcgtac gcggaggtca tcgaggacgc gacggccgcg caggtggcga
     1741 ggctgcagcc gggcgagacg ttcgtggtgc aggaggtcgc gcgcg
//
LOCUS       DASVOR010000218         5924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_31294,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000218 DASVOR010000000
VERSION     DASVOR010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31294"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF516_04820"
     CDS             <1..473
                     /locus_tag="VF516_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HEX8107026.1"
                     /translation="KPLFVAAVKRATSLDAEQGVLPGVLGSGKYGKDEIRLFEVIKQQ
                     VDKGAAITAFTRGQIEDAENVAEVEKGNAGETKVNGLAAAHEYSVLDYKPRNPKSGDP
                     ILVQLRNPWGKYGRKYVEERGAKKGVAVQGGDGVFWIDIADMVSFFGSLTFLKI"
     gene            complement(483..1103)
                     /locus_tag="VF516_04825"
     CDS             complement(483..1103)
                     /locus_tag="VF516_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107027.1"
                     /translation="MDSIPDEANALHQEGRKAGGAGDPARALELFARAHQLAPRWAYP
                     PYDMAFTYLLSDQIELAETWYALVDELEPRGFFTAKTSLDLIRRERRGELPRGFVKGF
                     IVLEWLPEDQQRAALQQIVTRLPGVAPAWKQLAGLTPDDDAKLVALDRGLAAHPDDET
                     YGVLVLNKALVLRRRGQQDAARRMLEDLLADRRCTRGVEAMARASL"
     gene            complement(1227..5372)
                     /locus_tag="VF516_04830"
     CDS             complement(1227..5372)
                     /locus_tag="VF516_04830"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8107028.1"
                     /translation="MSRTGCRDARSPGRRSPEVFIAYATADRAWALALRARLGRFHGV
                     FLDVIDIQAGDRWPEKIRSAEHHAVMTVVLVSRRSRHAYYQYDEIVYAVDAYRRQHLT
                     LVPVYLERWPGLDDVPYGLRQLQGILWTDKRRADDIAGLLSRHMLAWRSRTESGGGPE
                     LQTLEAPPWLPRATDYFLSDPELPPLLHGLAALSTNAALLIQNFLAWYLGTSAVKVPF
                     GGRGTQLGEFDRWLQGEDGPACRLLTAPAGQGKSAFLVHWAASVQASGAADVAFVPIS
                     ARFQTNVWSVFLPALTARLAAIHRIRLPPVDRDAAAEWKAAIARLVRQPPADGRRLLV
                     VLDGLDEAADWQPGPDIFPLDAPPHVRVVVSARTRPAKVPASVWLRLLGWDRTASAEA
                     VDLPPLRRADIGDVLRSMGAPLDELVHREAVIAELHRLSGGDPLVLRLYVDHLWSLRG
                     RFPTITPQFLRAMKPGIDGYFEDWWSQQEALWRSQGRPVDVRAVSDVLAALGCAFGPL
                     RRDDLARLVGQPDGDVLWRALAALGRFIVGDGDEHGYVFGHPRFASYVFEQRLLEHER
                     RAWDERFVAWGERFLDPAHADAANHEPVPAYLLRYLGAHLRRLEVPPEQTLRLLQPAW
                     CSAFEILDGHPAGFLAECAQVRNDLMRGNTAALDAGAAPPHLAALAAVEVRAGSVVAR
                     HVRISGALALDLVRAGVWTELTALAHARQPRREADKLVILEFLFDRMVEPWRSEIARE
                     FLDVLGGSRASVDMVQHAVQMWRKQGLSAEIRGRLEAFIRHCVAIARSNFEAALRAAL
                     EEPSEQLTLPTRSTTLDYPQARLCSALAALAHVLPHATGALVDEAADLVLRIAYPVAR
                     HRAIAAVAPLIVERPALQHKLVRQELAWTAAMPVLFRNLATYAEHVSEDVAAEMFEGV
                     DRMGSWKLAIDVCAALIPRLRAGLRDRAIDRLVDTLREGAEPLSPGYLRQVVAVAPQW
                     RQTLLDAIPAQVPSWFEESDRLRMAAELSPPAEACRLLRQVMEVQSHIDDTDVLVAAI
                     ASFPRIPTDLLAELEARYGQPGGDSARDNDLDELDQEHAHALAALATHVDRQTAIAYA
                     SQAFSSMGHVAERMSQTALASLVALLPDATRQELLDGEINTWRMMSDHQYFVGNATLV
                     AELLHRLPTQEADGHIAAIQEAASGAPHSLASILAILARRLEEPLATRLRETALGLAQ
                     RPGQQIWRGLALIAVADVLRPRDIDLLSDRLRRIHEDDDAHIILWMAALALAASRQRT
                     RLPLGILPDEGLVRTFALQRFLPHLSPAEQRIAVHELVETLSRDGEDPRTRIAIEAMI
                     PRWGQPQRYDLFCQLLRLVARGRLGIALIAPLLGQVTHALGGDELCEQVVTEIWRAGA
                     WWP"
     gene            complement(5451..>5924)
                     /locus_tag="VF516_04835"
     CDS             complement(5451..>5924)
                     /locus_tag="VF516_04835"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8107029.1"
                     /translation="PGRDGRGRGLAFARYKNQKAWCAVAVELTVDGGAAVRLARAAIA
                     ADAGQIGDPDGIVNQLEGGFVQAASWTLREAVTFDRTRVTSTDWQSYPILGFGDVPPI
                     ETVLLDRPGEPWLGAGEASQGPTAAAIANAIFDATGLRVRDLPLTAERLREAAWA"
BASE COUNT          970 a         2111 c         2018 g          825 t
ORIGIN      
        1 acaagccgct gttcgtcgcg gcggtcaagc gagccacctc gctcgacgca gagcagggcg
       61 tgctgcccgg cgtgctgggc agcgggaagt acggcaagga cgagatccgg ctgttcgagg
      121 tgatcaagca gcaggtcgac aagggcgcag cgatcacggc cttcacccgg gggcagatcg
      181 aggatgccga gaacgtggcc gaggtcgaga agggcaacgc gggcgagacc aaggtcaacg
      241 gcctcgcggc cgcccacgag tacagcgtgc tcgactacaa acccaggaac cccaagtcgg
      301 gcgacccgat cctggtgcag ctccgcaatc catgggggaa gtacggccgc aagtacgtcg
      361 aggagcgcgg cgcgaagaag ggtgtcgccg tgcagggcgg cgacggggtg ttctggatcg
      421 acatcgccga catggtgtcg ttcttcggaa gcctcacgtt cttgaagatc tgacggcctc
      481 gatcaaagcg acgccctggc catcgcctcc accccccggg tgcagcgccg gtccgcgagc
      541 aagtcctcga gcatccgccg agctgcatcc tgctggccgc gtcgacggag cacgagcgcc
      601 ttgttcaaca cgagcacgcc gtaggtctcg tcatcgggat gggccgccag gccgcggtcg
      661 agcgccacga gcttggcatc gtcatccggg gtcaatcccg ccagctgttt ccacgcgggt
      721 gcgacgccgg ggaggcgcgt cacgatctgt tgcaacgccg cgcgctgctg gtcctcggga
      781 agccattcga gcacgatgaa tcctttcacg aacccgcggg gcagctcccc gcggcgctcg
      841 cggcggatca ggtcgagcga ggtcttcgcc gtgaagaacc cgcgcggctc cagctcgtcg
      901 accagcgcat accaggtctc ggccagctcg atctgatcgc tcaacaggta cgtgaacgcc
      961 atgtcatagg gcggataggc ccatcgcggg gccagctgat gggcgcgcgc aaacagctcg
     1021 agtgcgcgcg ccggatcccc tgcaccgccc gccttgcgac cttcctgatg cagcgcgttc
     1081 gcctcgtccg ggatcgagtc catcgcggta tgctacgcga tgcgcgccgg gaaaccaccc
     1141 ggctcgcgct gtgcatgcca tgagcaccgc gacgagattc ggtatcgtcg agcccgtgct
     1201 tgcgcgcgtt gtacgtcgaa cgtcacctac ggccaccacg cgcccgcgcg ccagatctcg
     1261 gtgacgacct gctcgcatag ctcgtcgcca ccgagggcgt gcgtcacctg gcccagcagc
     1321 ggcgcgatga gtgcgatccc caggcggccg cgtgcgacca ggcgcaggag ctggcagaac
     1381 agatcgtagc gctgcggctg cccccagcga ggaatcatgg cctcgatggc gatccgtgtg
     1441 cgcggatcct cgccgtcgcg agacagcgtc tcgaccagct cgtgcaccgc gatccgctgc
     1501 tcggcgggcg acaggtgcgg caggaaccgc tgaagcgcga acgtccgcac gaggccctcg
     1561 tccggcagga ttcccagcgg caacctcgtc cgctgccggg acgccgccag cgccaatgcc
     1621 gccatccaca gaatgatgtg cgcgtcgtcg tcctcatgga tccggcgcag gcgatccgac
     1681 agcaggtcga tgtcgcgggg tcgcaacaca tccgccacgg cgatcagcgc gagcccgcgc
     1741 cagatctgct gcccgggccg ctgcgccagc ccgagcgcgg tctcgcgcag tctcgtcgcc
     1801 agcggctcct cgagccgccg agccaggatc gccaggatgc tggcgagcga gtgcggcgcg
     1861 ccgctggccg cttcctggat cgcggcgatg tgcccatcgg cctcctgcgt cggcagccgg
     1921 tgcagcagct ctgcgaccag cgtagcgttg ccgacgaagt actggtgatc actcatcatg
     1981 cgccacgtat tgatctcgcc gtccaggagc tcctggcgcg tggcgtcggg cagcagcgcg
     2041 accagcgaag ccagcgcggt ctgcgacatg cgctccgcga cgtggcccat cgagctgaac
     2101 gcctggctcg cgtacgcgat cgcggtctgg cgatcgacgt gcgtcgccag ggcagccagc
     2161 gcatgcgcat gttcctggtc gagctcatcg aggtcgttgt cccgagcgga gtctcctccg
     2221 ggctgtccgt agcgggcctc gagctccgcg aggaggtcgg tcgggatccg gggaaacgac
     2281 gcgatcgcgg cgaccagcac atccgtatcg tcgatatgcg actgcacttc catgacctgc
     2341 cgcagaaggc ggcacgcctc ggccggcggc gatagctcgg ccgccatgcg gagccggtcg
     2401 gactcctcga accagctcgg gacctgtgcg gggatcgcat cgagcagcgt ctgcctccac
     2461 tgcggggcga ccgcgacgac ctgccgaagg taccccggag acagcggctc ggcaccctcg
     2521 cgaagcgtgt cgacgagccg gtcgatggcc cggtctcgga gccccgctcg caaccgaggg
     2581 atcagcgcag cacatacgtc gatcgccagc ttccagctac ccatgcgatc taccccctcg
     2641 aacatctccg cggcaacgtc ctccgacaca tgctctgcgt aggtggcgag gttccggaac
     2701 aatacgggca tcgcggcggt ccatgcgagc tcctggcgca ccagcttgtg ctgcagagcg
     2761 ggtcgttcga cgatcagcgg cgcgaccgcc gcgatggcgc ggtgacgcgc caccggatac
     2821 gcaatgcgga gcaccagatc cgccgcctca tcgaccagcg cgcccgtcgc gtgcggcagc
     2881 acgtgtgcga gcgcggcgag cgcggagcac aaccgagcct gcgggtagtc cagcgtggtg
     2941 gaacgagtcg gcagcgtgag ctgctcggaa ggttcttcga gcgccgcgcg caatgccgcc
     3001 tcgaagttgg accgcgcgat cgccacgcag tgacggatga aggcttccag ccggccacgg
     3061 atctccgccg acagtccctg cttccgccac atctgcacgg cgtgctgcac catgtcgacg
     3121 gaggcacgcg acccacccag gacgtcgagg aattcccgcg cgatctctga ccgccagggc
     3181 tcgaccatgc ggtcaaacag gaattcgagg atgacgagct tgtccgcctc tctgcgcggc
     3241 tgccgcgcat gggccagcgc ggtcagctcg gtccagacgc cagcgcgcac caggtcgaga
     3301 gccagcgcgc cggaaatccg tacatggcgc gcgaccacgc tgcccgcgcg gacctcgacg
     3361 gctgccagcg ctgcgaggtg cggcggcgct gccccggcgt cgagcgcggc cgtgttacca
     3421 cgcatgaggt cgttgcgaac ctgcgcgcac tcggcgagga agccggcggg atggccatcg
     3481 aggatctcga acgcgctgca ccaggcgggc tggagtagcc gcagcgtctg ttcgggcgga
     3541 acctcgagcc tgcgcaggtg cgccccgaga tagcgcagga ggtacgccgg caccggctcg
     3601 tggttcgccg cgtccgcgtg cgccgggtcg agaaaccgtt ccccccacgc cacgaagcgc
     3661 tcgtcccagg cgcgccgctc gtgctcgagc agccgctgct cgaacacgta ggacgcgaac
     3721 cggggatggc cgaacacgta gccgtgctcg tcgccgtcac cgacgatgaa ccggccaagc
     3781 gccgcgagcg cacgccacag cacgtcgccg tcgggctgac cgacgagccg cgcgaggtcg
     3841 tcccggcgca gcggaccgaa cgcgcagccg agcgcggcca gcacatcgga gacggcgcga
     3901 acatcgaccg gccggccctg agaccgccac agcgcttctt gctggctcca ccagtcctcg
     3961 aagtagccgt cgatgcccgg cttcatcgcg cgaaggaact ggggggtgat cgtcgggaat
     4021 cggccgcgca ggctccacag gtgatcgacg tacaatcgca ggaccagtgg atcaccgccg
     4081 ctcagccgat ggagctcggc gatgaccgcc tcgcggtgca ccagctcgtc cagcggcgct
     4141 cccatcgagc gcagcacgtc gccgatatcg gcccgacgca acggaggcag atcgacggct
     4201 tccgcactgg ccgtccggtc ccatccgagc aagcgcagcc agacggacgc cggcaccttc
     4261 gccggccggg ttctcgccga caccacgacc cggacgtgcg gcggtgcgtc gagcgggaag
     4321 atgtccgggc ccggttgcca gtcggccgcc tcgtcgagcc cgtcgagcac gaccagcaac
     4381 ctccgtccat cggccggggg ctggcggacc agccgcgcga tcgcggcctt ccattctgcc
     4441 gcggcgtccc ggtccaccgg cggaagcctg atccgatgaa tcgcggcgag ccgcgccgtg
     4501 agcgccggca ggaagacgct ccagacgttg gtctgaaaac gcgcgctgat cggcacgaag
     4561 gccacgtccg ctgcgccgct ggcctggacc gacgctgccc agtgcaccag aaacgcggac
     4621 ttgccttgac cagcgggtgc ggtcagcaat cgacaggcgg gaccgtcctc cccttgcagc
     4681 cagcgatcga actccccgag ctgggtaccg cgaccgccga acggcacctt caccgccgag
     4741 gtcccgagat accacgccag gaagttctgg atcagcagcg ctgcgttggt cgacagcgca
     4801 gccagcccgt gcaagagcgg cggcagctct ggatccgaga ggaagtagtc ggtcgcgcgc
     4861 ggcagccagg gtggcgcctc gagcgtctgc agctcgggtc caccaccgct ttcggtccgt
     4921 gatcgccacg cgagcatgtg gcgggacagc aggccggcga tgtcgtcggc cctgcgcttg
     4981 tcggtccaca ggatgccctg aagctggcgc agaccgtagg gcacgtcatc gaggcccggc
     5041 caccgttcca ggtacaccgg gacgagcgtg aggtgctgtc ggcgatatgc gtcgaccgca
     5101 tagacgatct catcgtactg gtagtacgcg tggcgcgatc ggcgagacac cagcaccacg
     5161 gtcatcaccg cgtggtgctc tgcactccgg atcttctccg gccaccggtc gcctgcctgg
     5221 atatcgatga catcgagaaa cacaccgtgg aaacgaccga ggcgagcacg cagcgccagc
     5281 gcccatgcgc ggtccgcggt cgcatacgcg atgaacacct ccggcgagcg ccgacccggc
     5341 gatcgagcgt cgcggcatcc cgtcctcgac atgcgccgcc cgagcttacg acatcgcgca
     5401 ccacgaggac gcgatcgcag ccgtgtgctc ggtcgcggcc ggctcgaccg ctatgcccac
     5461 gcggcttcgc gcagccgctc cgcggtcagc ggcaggtcgc gcacccgcag gccggtcgcg
     5521 tcgaagatgg cgttggcgat cgccgccgcc gtcggcccct gcgacgcctc gccggcgccc
     5581 agccagggct cgcccggccg gtcgagcagc acggtctcga tcgggggaac atcgccgaac
     5641 ccgaggatcg ggtagctctg ccagtcggtg ctggtgacgc gcgtccgatc gaacgtcacc
     5701 gcctcgcgca gggtccagct cgcggcctgg acgaagccgc cctcgagctg gttcacgatc
     5761 ccgtcgggat cgccgatctg gccggcgtcg gcggcgatcg ccgcgcgcgc gagccggacc
     5821 gccgcgccgc cgtcgaccgt gagctcgacg gccaccgcgc accaggcctt ctggttcttg
     5881 tagcgcgcga acgcgagccc ccgaccgcgg ccgtcgcgcc ccgg
//
LOCUS       DASVOR010000219         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382010,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000219 DASVOR010000000
VERSION     DASVOR010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382010"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1270
                     /locus_tag="VF516_04840"
     CDS             <1..1270
                     /locus_tag="VF516_04840"
                     /inference="COORDINATES: protein motif:HMM:NF018449.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SBBP repeat-containing protein"
                     /protein_id="HEX8107030.1"
                     /translation="APGGDSYAVGYFDGETDLGPAGRHRAADNDKDPKHPGTDAFVVR
                     LDPDGKIVWGKTFGAGRDDVANGVAVRGDRVVVVGQFLDRLELGELKHTAAGSDDMFV
                     AAFDTRGEPLWLWTAGGVDSDGANAVAATPDGGWVVGGSFSQSVELQGAKLVSRGGTD
                     AMLIKLAATGDLEWVKQFGGRYNDTISHVAVDARGNVYLQGVFRDTADWGGKPLTAHG
                     GSDNDVVLAKYDANGDPLWSQGFGNAFNDVAGGLTVDPAGNVIMVGSFDRSVSFGPGD
                     DHASAGEADAFIARFTGDGKLTWAHSFGADREDIAFGVAADAAGNTVTTGWFQGSVDF
                     GTGAIASKGNKDVFALKLDPAGGLLWVRTWGDRDHDQGRAVAIDDKGCALVAGIYRFT
                     LALTSPPLESVRAEGDRIPKPDVFVIKLDR"
     gene            1385..1609
                     /locus_tag="VF516_04845"
     CDS             1385..1609
                     /locus_tag="VF516_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107031.1"
                     /translation="MAKAKKGKGGASRPTEMLLVASKVRQHIRDAGCNVAGDALDGLN
                     AYVAWLIDQAAKRASENGRKTVRTHDFIIM"
     gene            1690..>2231
                     /locus_tag="VF516_04850"
     CDS             1690..>2231
                     /locus_tag="VF516_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107032.1"
                     /translation="MGAPIYLVSACASGEEFVAAFRRYADKNGLFIPIGDPLPPGGRS
                     RFAVTLRDGGVMIEGEAEIVSSARAPSVLHGRVGMTLRFVAPDEPSRTTLTELEKARL
                     AMKPAPPSVPPRPADSPAEPRPVPPPVQGRIDAVNALAECVAIGDVSVLGPPLPSVTL
                     PPPKTGPKFVMPAIPAGRAAP"
BASE COUNT          324 a          801 c          798 g          308 t
ORIGIN      
        1 ggcgccgggc ggcgacagct acgcggtcgg ctacttcgac ggcgagaccg acctcggccc
       61 ggccggccgg caccgcgcgg ccgacaacga caaggacccc aagcaccccg gcaccgacgc
      121 gttcgtggtc cggctcgatc ccgacggcaa gatcgtctgg ggcaagacgt tcggcgccgg
      181 ccgcgacgac gtggccaacg gggtcgcggt gcgcggcgat cgcgtcgtgg tggtcggcca
      241 gttcctcgac cggctcgagc tcggcgagct caagcacacc gcggcgggct ccgacgacat
      301 gttcgtcgcc gcgttcgaca cccgcggcga gccgctgtgg ctgtggaccg ccggtggggt
      361 cgactcggac ggcgccaacg cggtcgccgc cacgccggac ggcggctggg tcgtcggcgg
      421 ctcgttcagc cagtcggtcg agctccaggg cgccaagctg gtgtcgcgcg gcggcaccga
      481 cgcgatgctg atcaagctcg ccgcgaccgg cgacctcgag tgggtcaagc agttcggcgg
      541 ccgctacaac gacacgatct cgcacgtcgc ggtcgacgcg cgcggcaacg tctacctcca
      601 gggcgtgttc cgcgacaccg ccgactgggg cggcaagccg ctgaccgcgc acggcggctc
      661 ggacaacgac gtcgtgctcg ccaagtacga cgccaacggc gaccccctgt ggtctcaggg
      721 cttcggcaac gcgttcaacg acgtcgccgg cgggctcacc gtcgatccgg ccggcaacgt
      781 catcatggtc ggctcgttcg accgcagcgt gtcgttcggt cccggcgacg accacgcctc
      841 ggccggcgag gccgatgcgt tcatcgcgcg gttcaccggc gacggcaagc tgacgtgggc
      901 gcacagcttc ggcgccgacc gcgaggacat cgcgttcggc gtcgcggccg acgccgccgg
      961 caacacggtg accaccggct ggttccaggg ctcggtcgat ttcggaaccg gcgcgatcgc
     1021 cagcaagggc aacaaggacg tgttcgcgct caagctcgat cccgccggcg gcctgctctg
     1081 ggtccggacc tggggcgatc gcgatcacga tcagggccgc gcggtcgcga tcgacgacaa
     1141 gggctgcgcg ctcgtcgccg ggatctaccg tttcacgctc gcgctgacct cgccgccgct
     1201 ggagtcggtg cgcgccgaag gcgatcgcat cccgaagccg gacgtgttcg tgatcaagct
     1261 cgaccgctag tacgtggatc agcgtagcgc agcgcgcgcg tcaaaacacc ccgtccgcca
     1321 gagccgcgca ccgccgggag ggtgtacaac caggcgacgc cactgccact cacagggaga
     1381 acacatggcc aaagcgaaga agggtaaggg cggcgccagc cgtccgacgg agatgctgct
     1441 cgtcgcctcg aaggtccgcc agcacatccg cgatgccggc tgcaacgtcg ccggcgatgc
     1501 gctcgacggc ctcaacgcct acgtcgcctg gctgatcgac caggccgcga agcgcgccag
     1561 cgagaacggg cgcaagaccg tccggacgca cgatttcatc atcatgtagg ccccgggctg
     1621 tcccggctcg ccgggcggtt gcgcccgtcg cattttctgg cgggtttgcc ttagaatgct
     1681 ggcccggtga tgggggctcc gatttatctg gtctccgcct gcgcgtccgg cgaggagttc
     1741 gtcgccgcgt tccggcggta tgcggacaag aacgggctgt tcatcccgat cggagatccg
     1801 ctgccgccgg gtgggcgaag ccggttcgcg gtgaccctgc gcgacggcgg cgtgatgatc
     1861 gagggcgagg cggagatcgt gagctcggct cgcgcgccgt cggtcctgca tggtcgggtc
     1921 ggcatgacct tgcgcttcgt cgcccccgac gagccgagcc ggacgacgct gaccgagctg
     1981 gagaaggcgc ggctggcgat gaagccggcg ccgccgagcg tgccgccgcg ccccgctgac
     2041 agtcccgccg agccccggcc ggtcccgccg ccggtccagg gccggatcga cgcggtgaac
     2101 gcgctcgccg agtgcgtcgc gatcggcgac gtctcggtgc tcggcccgcc gctgccctcg
     2161 gtgaccctcc caccgcccaa gaccggcccg aagttcgtga tgccggcgat cccggccggc
     2221 cgcgcggcgc c
//
LOCUS       DASVOR010000220         6831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_294448,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000220 DASVOR010000000
VERSION     DASVOR010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6831
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294448"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VF516_04855"
     CDS             <1..779
                     /locus_tag="VF516_04855"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX8107033.1"
                     /translation="RSRAGAQNQLVYWMYDTLGEQLPDPALSAIWLICLRNAVLRRPG
                     VLRVLGDRWRWKGPFAIADALFDRLLEHPEGTEIAHVDIASNLADHVRYRDGKIRLAP
                     AAMLRELQRALDTPLAVDREFPLVLSAGLRSLWTANAIHRDPAWRKGKGPHCAISIST
                     ADAADLGLVHDEPAVLRTRHGAVTLPVTIDPRLSAGHVSLPNGFGLTYGGARDGANLN
                     ELTSAEDRDPFTACPHHKWLPCRIERLPRTEPERATPRSP"
     gene            908..1504
                     /locus_tag="VF516_04860"
     CDS             908..1504
                     /locus_tag="VF516_04860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8107034.1"
                     /translation="MSEHDPHSMNPDADVRIAVQAGDRRRAAAVLVARYGDVVYRYVL
                     AMTGNRHLAEDIHQQVFLEAYRDLECFGGRSSVRVWLLGIARHRCIDALVVTRRWNDR
                     FKNEFSAETEADDCAADRKIDRCRMQRILAGCLTRLTPASREAVMLRYQEELSYEEAA
                     AVAGVCAATLQKRVTRALPLLRRYIRAELCSGAAARAG"
     gene            complement(1461..5252)
                     /locus_tag="VF516_04865"
     CDS             complement(1461..5252)
                     /locus_tag="VF516_04865"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8107035.1"
                     /translation="MFTGNSRFRLIRKIGSGGMGVVYEAEDLLRKTRVALKTLYQTDA
                     DLLYRLKREFRSLRDVVHRNLISLDELFEEAGDWFFTMELLDGENLNAYLQRTAMSPL
                     APPDGDTPAWRLPGMDEDVATETIDRPPPGDAAPTPPTKRELRNAPPLDFERIRAVFA
                     QITEGLLTIHDAGKIHRDIKPSNVFVTRDGRVVILDFGLVTEDWEMARSREGEIVGTV
                     MYMAPEQAAGMAVGPQADWYSLGVMLYEVIAGRRPFDGPFGVVLAAKQYSAPRDPKTI
                     NPHCPDDLVQLCLQLMRVEPLQRPSGLDVLRQLGAGKHEAAAQIAPVRPSQRGIFIGR
                     EPELAELHRAQGDVAGGAAVAVFVHGESGIGKSTLIHHFISTVSSEGAELVVLMGQCR
                     EHEAVPFKAMDGIIDALSRFLCRVSEVAAAELVPKNAALLPGAFPVLGRVAAIAKAPR
                     PAQVAAEPFQQRKRVFSALREMLCRLTERYRLICVIDDMQWADSDSLALLEELLRPPD
                     EPRMLFLASLRSEDEAPPLPPLPGEVRRLRLSRLNPEESVRLAGLLLDRVAPEQKLQT
                     ARIVDETAGHPLFIGELVRYAAADHQAAIRQLRLDEAIWARVSQLPPAALDVLKILAV
                     AVAPLSEELIYVAAQLEPSDFQRCVATLRVSHLIRSAVSAHTSLEIYHDRVRQAVSRR
                     LDDPERVALNQRCALALETSSAAIQPELLIYHLEGAREFDQAARKAVEAADRAQAGLA
                     FEQAARLYEAALRLARWSDDERRQILIKLGGTLASAGRGPEAAKAYHEAAVGADPTTQ
                     LTCRIQGADQLVQSGQLASGASLLFSLFEEHGHAVPRSQMQITLGIAWYRLRLALHGL
                     RWTDRSRNEIQPRDLALLALYKAAGRGLTLVDPFRAAYFVIRGLNLAIAIGDRDSIMD
                     FLLLEAGFRGGEGGKEAKFLKRADAVMREHPDPAFQTSYRLSLGTRRYTSMDREFKNT
                     FEMLDRSDQELSQTANAAWYLSAGRFFLIHSLRKMGDFARLKTYSERFIREAEERGNV
                     YGRTTLSRLCNILWLVDDDPQGAREELKKHSWMPYSEGYHSQHWLELMALVEIAIYEG
                     SSVDEDFFFQHIRGLKKSVHGRVLAYRCDAAWLVGRRALSELARDPSQARAVRRSIAS
                     LVSYNTQYSRVLAGMLRATLAAHDDDGDAAIAQFRDVMTLAELTHVFFIAAVARRRLG
                     LLIDGDEGKELVIAAEHWMRAAGIQNVERMTTLVSPIGRRAPARRVTRPERLLPSKAP
                     L"
     gene            5627..>6831
                     /locus_tag="VF516_04870"
     CDS             5627..>6831
                     /locus_tag="VF516_04870"
                     /inference="COORDINATES: protein motif:HMM:NF024306.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like protein"
                     /protein_id="HEX8107036.1"
                     /translation="MSAPKIRVEHRESLAFALSEAAELEHGLLCCYLYTAYSIGPRRE
                     RRHGPEQAAAIARWRAEITDIARDEMIHLGLVSNLLNAIGAAPHFMRPNFPVASGYHP
                     AGIVVSLAPFCLPTIQHFVYLERPEDVIEREGEGFARRPYTRIGHAGRLVPSGQDYTT
                     QGDLYRWIRDGFAHLVELHGEAQVLCGDPRLQVTSSQLGLPGITAVTDLASAEGALDA
                     IVIQGEGAPRCPGHSHYSRFKAIRDELQAVLAADPAFAPAAGVAYNPVMRQPANPEGL
                     VWVDDEPAASVLDVANAIYAFTLRALGVLYSPVALADEPRALALEAVHAGMRGLTPIG
                     ELLTELPARRSDPSVRAGVSFAMSRSLHPLPEPRAALRTLYEAISAVAGGIRSHVVPL
                     DAGQLVTAER"
BASE COUNT          954 a         2406 c         2477 g          994 t
ORIGIN      
        1 tgcgctcgcg cgccggggcg cagaaccagc tcgtgtactg gatgtacgac acgctcggcg
       61 agcagctgcc cgatcccgcg ctgtcggcga tctggctgat ctgcctgcgc aacgccgtgc
      121 tgcggcggcc cggcgtgctg cgggtgctgg gggaccgctg gcggtggaag ggcccgttcg
      181 cgatcgccga cgcgctgttc gaccggctgc tcgagcaccc cgagggcacc gagatcgcgc
      241 acgtcgacat cgcgtccaac ctggccgacc acgtgcggta ccgcgacggc aagatccggc
      301 tcgcgccggc cgcgatgctg cgcgagctgc agcgcgcgct ggacacgccg ctggcggtgg
      361 accgggagtt tcccctcgtg ctgtccgcgg ggctgcgcag cttgtggacg gccaacgcga
      421 tccatcgcga tccggcgtgg cgcaagggca aggggccgca ctgcgcgatc tcgatcagca
      481 cggccgacgc cgcggacctc ggcctggtgc acgacgagcc cgcggtgctg cgaacccgtc
      541 acggcgcggt caccctgccg gtgacgatcg acccccggct gtccgccggc cacgtctcgc
      601 tgcccaacgg cttcggcctg acctacggcg gcgcgcgcga cggcgcgaac ctcaacgagc
      661 tcacctcggc ggaggatcgc gatccgttca ccgcgtgccc gcaccacaag tggctgccgt
      721 gccggatcga gcgactgccg cgcaccgagc cggagcgagc gaccccccgt tcaccctgac
      781 ccagatcctt tcatcccgag atccgtacaa gcgatcgatg ccgcgcaccg ggccggcggt
      841 cctgccgcgc gccatcgcga cgcgcaaatc gaggtgataa cgtatgatga atggccatga
      901 tcatcagttg tcagagcatg atccgcactc gatgaacccc gacgccgacg tgaggatcgc
      961 ggtgcaggcc ggcgaccggc ggcgcgccgc ggcggtcctg gtggcgcgct acggagatgt
     1021 ggtgtaccgc tacgtgctcg cgatgaccgg aaatcgccat ctcgcggagg acatccatca
     1081 gcaggtgttc ctggaagcgt atcgcgatct ggagtgcttc ggcggccgat cgtcggtgcg
     1141 ggtgtggctg ctcgggatcg cgcgccaccg ctgcatcgac gcgctggtgg tgacccggcg
     1201 gtggaacgac cggttcaaga acgagttctc cgccgagacc gaggccgacg attgcgcggc
     1261 cgaccgcaag atcgatcgct gccggatgca gcgcatcctc gccgggtgcc tgacccggtt
     1321 gacgccggcg tcgcgggagg cggtgatgct gcgctatcag gaggagctgt cgtacgagga
     1381 ggccgcggcg gtcgccgggg tgtgcgccgc cacgctgcag aagcgcgtga cccgggcgct
     1441 tccgctgctg cgccggtaca tcagagcgga gctttgctcg ggagcagccg ctcgggccgg
     1501 gtgacgcggc gcgccggtgc gcgccggccg atcggcgaca ccagggtcgt catgcgctcg
     1561 acgttctgga tccccgcggc gcgcatccag tgctcggccg cgatgacgag ctccttgccc
     1621 tcgtcaccgt cgatcagcag gccgagccgg cggcgggcga ccgcggcgat gaagaacaca
     1681 tgggtcagct cggcgagcgt catcacgtcg cgaaactgcg cgatcgccgc gtcgccgtcg
     1741 tcgtcgtgcg cggcgagcgt ggcgcgcagc atgccggcga gcacccggga gtactgggtg
     1801 ttgtaggaca ccaggctcgc gatcgacctg cgcacggcgc gggcctgcga cgggtccctg
     1861 gcgagctcgg acagcgccct gcgcccgacc agccaggcgg cgtcgcagcg ataggcgagg
     1921 accctgccgt ggaccgactt cttgaggccg cggatgtgct ggaagaagaa atcctcgtcg
     1981 accgacgagc cctcgtagat cgcgatctcg accagcgcca tgagctcgag ccagtgctgg
     2041 gagtgatagc cctcgctgta cggcatccag gagtgcttct tgagctcctc gcgcgcgccc
     2101 tgggggtcgt cgtcgaccag ccacaggatg ttgcacagcc ggctcagcgt cgtccggccg
     2161 tacacgttgc cgcgctcctc ggcctcccgg atgaaccgct ccgagtaggt cttcagccgc
     2221 gcgaagtcgc ccatcttgcg caggctgtgg atcaggaaga accgcccggc cgacaggtac
     2281 cacgcggcgt tcgcggtctg gctgagctcc tggtcgctgc gatcgagcat ctcgaacgtg
     2341 ttcttgaact cgcgatccat cgacgtgtac cggcgggtac ccaggctcaa ccggtagctg
     2401 gtctggaacg ccggatcggg gtgctcgcgc atgaccgcgt ccgcgcgctt gaggaacttg
     2461 gcttccttgc ccccctcgcc gccacggaat ccggcctcga gcaggaggaa gtccatgatc
     2521 gagtcgcgat cgccgatcgc gatcgccagg ttgagcccgc ggatcacgaa gtaggcggcg
     2581 cggaatggat cgaccagggt cagcccgcgg cccgccgcct tgtacagcgc gagcagcgcc
     2641 aggtcgcggg gctggatctc gttccgggag cggtcggtcc agcgcagccc gtggagcgcc
     2701 aggcgcagcc ggtaccatgc gatcccgagc gtgatctgca tctgcgaccg cgggaccgca
     2761 tggccgtgct cctcgaacag cgagaacagc aggctggccc cgctcgccag ctggccgctc
     2821 tggacgagct ggtcggcgcc ctggatccgg caggtcagct gggtggtcgg atcggcgccc
     2881 accgcggcct cgtggtaggc cttcgcagcc tcggggccgc gtcccgccga ggcgagggta
     2941 ccgccgagct tgatcaggat ctgccggcgc tcgtcgtccg accaccgggc gaggcgcagc
     3001 gcggcctcgt acagccgcgc ggcctgctcg aacgcgaggc cggcctgtgc gcggtccgcg
     3061 gcctcgaccg ccttgcgcgc ggcctggtcg aactcgcgcg cgccctcgag gtggtagatc
     3121 agcagctcgg gctggatcgc ggcgctcgag gtctccagcg cgagggcgca gcgctggttg
     3181 agcgccacgc gctcggggtc gtcgaggcgg cggctcaccg cctggcgcac tcgatcgtga
     3241 tagatctcga gcgaggtgtg cgcggacacc gcgctgcgga tcaggtggct cacgcgcagc
     3301 gtggcgacgc agcgctggaa gtcgctgggc tcgagctgcg ccgcgacgta gatcagctcc
     3361 tccgagagcg gcgcgacggc gacggcgagg atcttgagca cgtcgagcgc ggcgggcggg
     3421 agctgcgaca cgcgggccca gatcgcctcg tccaggcgca gctggcggat cgcggcctgg
     3481 tggtcggcgg cggcgtagcg cacgagctcg ccgatgaaca gcgggtggcc ggcggtctcg
     3541 tcgacgatgc gcgcggtctg cagcttctgc tcgggcgcga cgcggtcgag cagcagcccc
     3601 gcgagccgga cggactcctc ggggttcagc cgcgaaagcc ggaggcgccg gacctcaccc
     3661 ggcagcggcg gcaacggcgg cgcctcgtcc tcggagcgca gcgacgccag gaacagcatg
     3721 cggggctcgt cgggcggccg cagcagctcc tcgagcaggg ccaggctgtc gctgtcggcc
     3781 cactgcatgt cgtcgatgac gcagatcagg cggtagcgct cggtcagccg gcacagcatc
     3841 tcgcgcagcg cgctgaacac ccgcttgcgc tgctggaacg gctcggcggc gacctgggcc
     3901 gggcgcggcg ccttcgcgat cgcggcgacc cggccgagca ccgggaacgc gccgggcagc
     3961 agcgccgcgt tcttgggcac cagctcggcc gccgcgacct ccgacacgcg acacaggaag
     4021 cgcgacagcg cgtcgatgat gccgtccatc gccttgaacg gaacggcctc gtgctcgcgg
     4081 cactggccca tcagcacgac cagctccgcg ccctcgctgc tcaccgtcga gatgaagtgg
     4141 tggatcagcg tgctcttgcc gatcccggac tcgccgtgga cgaacaccgc gacggcggcg
     4201 ccgccggcga cgtcgccctg cgcgcggtgg agctcggcga gctcgggctc gcggccgatg
     4261 aagatcccgc gctgcgacgg ccggaccggc gcgatctggg cggcggcctc gtgcttgccc
     4321 gcgccgagct ggcgcaggac gtcgaggccc gacgggcgct gcagcggctc gacccgcatg
     4381 agctgcaggc acagctgcac gaggtcgtcg gggcagtgcg gattgatggt cttgggatcg
     4441 cggggcgcgc tgtactgctt ggcggccagc accacgccga acggtccatc gaacggccgc
     4501 cgcccggcga tgacctcgta cagcatgacg ccgaggctgt accagtcggc ctgcggcccg
     4561 accgccatgc cggcggcttg ctccggcgcc atgtacatca cggtgccgac gatctcgccc
     4621 tcgcgcgacc gcgccatctc ccagtcctcg gtgaccagcc cgaagtcgag gatcacgacc
     4681 cggccgtccc gggtgacgaa cacgttcgag ggcttgatgt cgcggtggat cttgccggcg
     4741 tcgtggatcg tgagcagacc ctcggtgatc tgcgcgaaca ccgcgcggat ccgttcgaag
     4801 tcgagcggcg gcgcgttgcg cagttcgcgc ttggtcggcg gcgtgggcgc ggcatcgccg
     4861 ggcggcgggc ggtcgatggt ctcggtggcc acgtcttcgt ccatgccggg caggcgccac
     4921 gccggtgtgt cgccgtcggg cggcgcgagc ggcgacatcg cggtgcgctg caggtaggca
     4981 ttcaggttct cgccgtcgag cagctccatc gtgaagaacc agtcgccggc ctcctcgaac
     5041 agctcgtcga ggctgatcag gttgcgatgg acgacgtcgc gcaggctgcg gaactcgcgc
     5101 ttgagccggt acagcaggtc cgcgtcggtc tggtacagcg tcttgagcgc gacccgggtc
     5161 ttgcgcagca ggtcctccgc ctcgtagacc acgcccatcc caccggagcc gatcttgcgg
     5221 atgagccgga agcggctatt gccggtgaac atcgctgtct cccgcgcacg accatcgctg
     5281 cgtgttcagc ggtcccaccc gggtcgagtc tggcggatgg aggtcgaagc gcaaccgcgc
     5341 ggaagtccca ccgtatgcga ccgtgacccc gacacccgcg gggagactcg gcgggacacc
     5401 gccggcttgc ggagagccgt tctcggtggc agagctcacc ggcggccatc ttacgctcgg
     5461 tcctcgccct ggtgccatgt atgtccaggt aaatgcagga gctatggggt acggccgggg
     5521 atgcgtgggc ggtgcggaca ggggcgatct gcaccggtgg ggaacgcacc ccgatcgcgt
     5581 cgggtccgcg tgcatccggg cgccgagctg ccatgatgtc gcccgcgtga gcgcgccgaa
     5641 gatccgggtc gagcaccgcg agagcctggc ctttgcgctg agcgaggccg ccgagctcga
     5701 gcacggcctg ctgtgctgct acctgtacac cgcgtacagc atcggcccgc gccgcgagcg
     5761 ccgccacggc cccgagcagg ccgcggcgat cgcgcgctgg cgcgccgaga tcaccgacat
     5821 cgcgcgcgac gagatgatcc acctcggcct ggtgtcgaat ctgctcaacg cgatcggcgc
     5881 cgcgccgcac ttcatgcgcc cgaacttccc ggtcgcgtcg ggctatcacc cggccggcat
     5941 cgtggtgtcg ctcgcgccgt tctgcctgcc gacgatccag cacttcgtct acctggagcg
     6001 gcccgaggat gtgatcgagc gcgagggcga gggcttcgcg cgccggccct acacgcggat
     6061 cggccacgcc ggtcggctgg tgccgagcgg gcaggactac acgacccagg gcgacctcta
     6121 ccgctggatc cgcgacggct tcgcccacct ggtcgagctc cacggcgagg cccaggtgct
     6181 gtgcggcgat ccgcggctgc aggtgacgtc gtcgcagctc ggcctgcccg gcatcaccgc
     6241 ggtgaccgat ctggcgtccg ccgagggcgc gctcgacgcc atcgtgatcc agggcgaggg
     6301 tgcgccgcgc tgcccggggc attcgcacta cagccggttc aaggcgatcc gcgacgagct
     6361 gcaggcggtg ctcgccgccg acccggcgtt cgcgccggcg gcgggcgtcg cctacaaccc
     6421 ggtgatgcgg cagcccgcca accccgaggg gctggtgtgg gtcgacgacg agccggcggc
     6481 cagcgtgctc gacgtcgcca acgcgatcta cgcgttcacg ctgcgcgccc tgggtgtgct
     6541 gtactcgccg gtcgcgctcg ccgacgagcc gcgcgcgctc gcgctcgagg cggtccatgc
     6601 cgggatgcgc ggcctgaccc cgatcggcga gctcctgacc gagctgccgg cgcggcgctc
     6661 ggatccgtcg gtgcgcgccg gcgtcagctt cgcgatgtcg cgctcgctcc acccgctgcc
     6721 cgagccgcgc gccgcgctgc gtacgctgta cgaggcgatc tccgcggtcg ccggcgggat
     6781 ccgcagccac gtcgtgccgc tcgacgccgg ccagctcgtc accgccgagc g
//
LOCUS       DASVOR010000221         4308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_2228,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000221 DASVOR010000000
VERSION     DASVOR010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2228"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1087)
                     /locus_tag="VF516_04875"
     CDS             complement(104..1087)
                     /locus_tag="VF516_04875"
                     /inference="COORDINATES: protein motif:HMM:NF021640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide-binding protein"
                     /protein_id="HEX8107037.1"
                     /translation="MDRPRRLVRTRGVGRKQTAATRRDQIARRIYVSMPADPWLTDNL
                     NHLKWGVVDEIARLGYIPEIFTNPRGMPGLASSKVWSPGIADEIARRCTGAVILGMPR
                     WKFQDELGEPAFLPTEFNHYEGALARTLGLPTLVLVQKDLRRRVVFDASFGGYVGEFD
                     AEADARWLHTDEFRVPFQYWTELLEQRRDVFLGYCSRSEQTAAAIKDYLICLGTKVLD
                     WQTDFLPGRTILDQIEQAAARSVGGIFLFTNDDDLAEVPGQEPQAVPRDNVVFEAGYF
                     IGLKGKRNVLIVREHGSKMPADLGGDIYAPLLDKTNIKPIKKTLAAFLSTF"
     gene            complement(1178..2134)
                     /locus_tag="VF516_04880"
     CDS             complement(1178..2134)
                     /locus_tag="VF516_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107038.1"
                     /translation="MRRSLAALALCGLAGVAHADRADQLFKKGQKLLGEKRYAEACAA
                     FEDSDRLDPEIGAKLNVARCYQEWGKLATAWRWYTDAEQMASKTGDPRTPKIHARIEE
                     LDRDVPRLTLVLPPDAATDHLAIQLDGGAVALATLGDERRIDPGPHEITILVDGKPRT
                     QTVPVQRGDRAKVKLDIPARPRARRTSKPEVVADPGSTRRLIGLGVSGAGVVAVGIAG
                     IATLNARSDYQHALHGYCNDAPDMCDATGLSRTHSARHRANIATAVTLLGTAAVGGGL
                     YLYFTAPGTAAAAERPERASRALYLAPAIGGDGAGVVLGGAF"
     gene            complement(2131..3654)
                     /locus_tag="VF516_04885"
     CDS             complement(2131..3654)
                     /locus_tag="VF516_04885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107039.1"
                     /translation="MVRLSSVLYRVLAAAAVAAAVGCAQLAGIQDTNGNARPGASVAV
                     TRMSVGSKIVLAPLDPGQLPATYFVASSDPSGFDRIPAVPDPVHGGWTTKLRAAAPVE
                     FTLPDVPTPIPRLFAFPSPQLSVLYGVLEHPNPQAAPMGATFAVSATLDAPVAAGQSF
                     QTYVVGAWLARGFSAAELPVPPSQQLGPLTIAFTAANSIPGRGQIDQVTPDDAFLILR
                     YTGAALTGVAEAPAFAQTDMATTVPPATMTAVPQDQKLDVKITPATIAARYTKVAPAV
                     ATLQMSWSLVAAPGSSIASNTGPALLSGGVMMTDPGVSVSYGNPFVARGWNTIFTLAT
                     SESRVYAPMGPMGMPTPITLFAGMNQFFDPAHATDLELPAGLPQSITFGDVLLTSDIV
                     TVKAPTKFVDVSFTVDTPMLSTGPAPSPTIYELQVFDLVVNSTATGLDRRFVLDAVSD
                     APKFALPPEVFQAGHSYSLRAIADLGGFPTVDQGNFLNRQLPLAQGYLDGGVVTVMP"
     gene            complement(3772..>4308)
                     /locus_tag="VF516_04890"
     CDS             complement(3772..>4308)
                     /locus_tag="VF516_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107040.1"
                     /translation="TIRHRLERLVPELVRKTFAAGLGAVFSTEEGIRKIARESLPEAV
                     GYIASSADGAKDKVFEVIARETREFLSNLNVSEEIAKILTTLSFEIKTEIRFIPNSDR
                     FVGAEPDVKASVRLKRQDEKPERGDKSEKDKSEKKDDEREGRSRLRFWQRDKDSDAGD
                     GDAERSDGRSEGSRDRKS"
BASE COUNT          613 a         1501 c         1515 g          679 t
ORIGIN      
        1 gccgaggccg ccgagtccgc cgcggtcaac cgcgccatcc tcgcccggct cgacgacatc
       61 gtccgcctgc tcggcgaggg caagccgtga gcgtcccgcg cgttcagaac gtgctcagga
      121 acgctgccag cgtcttcttg atcggcttga tgttcgtctt gtccaaaagc ggcgcataga
      181 tatcaccgcc cagatcggcg ggcatcttcg acccatgctc gcggacgatg aggacgttcc
      241 gcttgccctt gaggccgatg aagtacccgg cctcgaagac gacattgtca cgaggcactg
      301 cctggggctc ctgcccgggc acctcggcaa gatcgtcatc gttggtgaac aggaagatgc
      361 cgccgaccga ccgggccgcg gcctgctcga tctggtcgag gatcgtccgc cccggaagga
      421 agtcggtttg ccagtccagc accttggtgc cgaggcagat caggtagtcc ttgatggcag
      481 ccgcggtctg ttccgagcgg ctgcaatagc cgaggaatac gtcccggcgc tgttccagaa
      541 gctccgtcca gtactggaac ggcacacgga actcgtcggt atgcagccac ctggcgtcgg
      601 cctcggcatc gaactcgccg acgtatcctc cgaagctcgc gtcgaacacc acccgtcggc
      661 gcagatcctt ctgcacgagc accagggtcg gaagacccag cgtgcgggcc agcgccccct
      721 cgtagtggtt gaactcggtc ggcaggaagg ccggctcccc gagctcgtcc tgaaacttcc
      781 agcgcggcat tcccaggatc acagctcccg tgcagcgcct cgcgatctcg tccgcaatgc
      841 ccggactcca gaccttggag gatgccaggc ccggcattcc ccggggattc gtgaagatct
      901 cggggatgta gccgagcctg gcgatctcgt ccacgacgcc ccacttgagg tgattgaggt
      961 tgtcggtgag ccaggggtct gccggcatgc tcacgtaaat gcggcgcgcg atctggtctc
     1021 gccgggtcgc tgcggtctgc ttgcgaccga cgccgcgggt acggaccagc cgccggggac
     1081 ggtccacggt cctgcgtcgc ggagttgtct tcgaacgagc gatggcgcac ctccgatcgg
     1141 cgagcctacc atcttgacgc gatcgccgaa tgtagaatca gaacgcgccg ccgagcacga
     1201 cgccggcacc gtcgccgccg atcgccggcg cgagatacag cgcgcggctg gcgcgctcgg
     1261 ggcgctcggc ggcagcagcg gtcccgggcg ccgtgaagta cagatagagc ccgccgccga
     1321 ccgccgccgt gccgagcaag gtcaccgcgg tcgcgatgtt ggcgcggtgg cgtgcgctgt
     1381 gggtgcggga caggccggtg gcatcgcaca tgtcgggcgc gtcgttgcag tagccgtgca
     1441 gcgcgtgctg gtagtcgctg cgggcgttga gggtcgcgat gccggcgatg ccgaccgcga
     1501 ccacgccggc ccccgacacg ccgagcccga tcaggcggcg ggtgctgccg gggtccgcga
     1561 ccacctcggg cttcgaggtg cgccgcgccc gcggccgggc gggaatgtcg agcttgacct
     1621 tggcgcggtc gccgcgctgc accgggaccg tctgggtccg cggcttgcca tcgacgagga
     1681 tcgtgatctc gtgcgggccg ggatcgatcc ggcgctcgtc gcccagcgtc gcgagcgcca
     1741 ccgcgccacc gtcgagctgg atcgccaggt ggtcggtggc cgcgtcgggc ggcagcacca
     1801 gggtcaggcg cggcacgtcg cggtcgagct cctcgatccg ggcgtggatc ttgggcgtgc
     1861 gcggatcgcc ggtcttgctc gccatctgct cggcgtcggt gtaccagcgc caggcggtcg
     1921 cgagcttgcc ccactcctgg tagcagcgcg cgacgttgag cttggcgccg atctcgggat
     1981 cgaggcgatc gctgtcctcg aacgccgcgc acgcctcggc gtagcgcttc tcgccgagca
     2041 gcttctggcc cttcttgaac agctggtcgg cgcgatcggc gtgggccacg ccggccaggc
     2101 cgcacagcgc gagcgcggcg agcgaccgcc tcatggcatc accgtgacca cgccgccgtc
     2161 gaggtagccc tgcgcgagcg gcagctggcg gttgaggaag ttgccctggt cgacggtcgg
     2221 gaagccgccg agatccgcga tcgcgcgcag cgagtagctg tgaccggcct ggaacacctc
     2281 gggcggcagc gcgaacttcg gtgcgtcgct caccgcgtcg agcacgaagc ggcggtcgag
     2341 ccccgtcgcc gtcgagttga ccaccaggtc gaagacctgg agctcgtaga tcgtcgggct
     2401 cggggccggc ccggtggaca gcatcggcgt gtcgacggtg aagctcacgt cgacgaactt
     2461 cgtcggcgcc ttcacggtca cgatgtcgga cgtcagcagc acgtcgccaa acgtgatcga
     2521 ctgcggcagc ccggccggca gctcgaggtc agtggcgtgc gcggggtcga agaactggtt
     2581 catcccggcg aacagcgtga tcggcgtcgg catgcccatc ggccccatcg gggcgtacac
     2641 ccggctctcg gacgtcgcca gcgtgaagat cgtgttccag ccccgggcga cgaacgggtt
     2701 gccgtacgac accgagaccc caggatcggt catcatcacg ccaccgctca gcagtgccgg
     2761 tcccgtgttc gacgcgatcg aggatccggg cgccgcgacc aggctccagc tcatctgcag
     2821 cgtcgcgacc gcgggggcga ccttggtgta gcgcgcggcg atcgtggcgg gcgtgatctt
     2881 cacgtcgagc ttctggtcct ggggcaccgc ggtcatcgtc gcgggcggca ccgtggtggc
     2941 catgtcggtc tgcgcgaacg ccggcgcctc ggcgacgccg gtgagggcgg ccccggtata
     3001 gcgcaggatc aggaacgcat cgtcgggcgt gacctgatcg atctgcccgc ggcccgggat
     3061 gctgttcgcc gccgtgaacg cgatcgtcag cggcccgagc tgctgcgaag ggggcaccgg
     3121 aagctcggcg gccgagaacc cgcgcgcaag ccatgcgccg accacgtagg tctggaagct
     3181 ctggcccgcg gcgaccgggg catcgagcgt cgcgctgacc gcgaacgtcg cgcccatcgg
     3241 cgcggcctgc gggttggggt gctccagcac gccgtacagc accgacagct gcgggctggg
     3301 gaacgcgaac agccgcggga tcggcgtggg cacgtcgggc agcgtgaact cgaccggcgc
     3361 cgcggcgcgc agcttcgtgg tccatccgcc gtggaccgga tcgggtaccg ccgggatgcg
     3421 atcgaacccg ctcggatcgc tgctcgcgac gaagtacgtc gccggcagct ggcccggatc
     3481 gagcggcgcc aacacgatct tgctgccgac cgacatccgc gtcaccgcaa ccgaggcgcc
     3541 cggccgtgcg ttgccgttgg tgtcctggat cccggcgagc tgtgcgcagc cgaccgcggc
     3601 cgccaccgcg gcggcagcga gcacccggta caggacactg gacagccgca ccatggcgcg
     3661 atgctatcat cgcaccgcca gcgcgaaggg cgccggcgcg tctgctgtcg cggcggtcac
     3721 gttccggcac gttccggcac gccccggtgg gcgcgccgcg tttcgccggt gtcaggactt
     3781 gcggtcgcgg ctcccctcgg agcgcccgtc ggagcgctcc gcgtcgccat cgccggcgtc
     3841 gctgtccttg tcgcgctgcc agaaccgcag gcgcgaccgg ccctcgcgct cgtcgtcctt
     3901 cttctcgctc ttgtccttct cgctcttgtc ccccctctcc ggcttctcgt cctggcgctt
     3961 gagccgcacc gacgccttga cgtcgggctc ggcgccgacg aaccgatcgc tgttggggat
     4021 gaaccggatc tcggtcttga tctcgaacga cagggtcgtc aggatcttgg cgatctcctc
     4081 gctgacgttg aggttggaca ggaactcgcg ggtctcgcgc gcgatcacct cgaacacctt
     4141 gtccttggcg ccgtcggccg acgaggcgat gtagcccacg gcctcgggca ggctctcccg
     4201 cgcgatcttg cggatgccct cctcggtcga gaacaccgcg ccgagcccgg cggcgaacgt
     4261 cttgcgcacc agctcgggca ccagccgctc cagccggtgg cggatggt
//
LOCUS       DASVOR010000222         4170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410624,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000222 DASVOR010000000
VERSION     DASVOR010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410624"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2078)
                     /locus_tag="VF516_04895"
     CDS             complement(<1..2078)
                     /locus_tag="VF516_04895"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8107041.1"
                     /translation="MEAALGVSFAAVRVHQSAYAQANGAQAFTRGADIFFAPGRFDPI
                     SPQGLELIGHELTHVKQQAEGRVPVTAQIDGAPANDDPALEREADDLGAKAARGTPAQ
                     GAAPQAGAGPQPASAPTAAGDHAPRQAKADADLVDAAPPDARPRVEELAGPEAAAALD
                     VGAGSAPPIQKAPAGQPQHGAKGKKEFIPFRIAVGKPMTREEFEAAANLQVFGTTAIP
                     SQWHNVKDAYTPADSPVEVLFEASLVRRVRGAASAARGIDTDATGNVAGADARAKDFQ
                     AQPASGQKAALLAEIDRRYHAASGTAPGTQIQPNESGKAELWRSIRDEVLFQHQYLAN
                     LPDKVKALIRISIQGRDLTPADYDQLFRIAKKIEALPPGAAADYTSKITGSTADLTTF
                     EAAIDGYRGELAAREQADADRTTVQNKLLGLEEVYRLYREYRTTPIEAAPPGMREQLE
                     QQLARYGFPSIDEFAAYIARFEQAFEDGAVRITLDVLARYAGKLYQESQRYQDAAVVK
                     ELHARLGGFRTQFQEFDQNARIWNDYAAQANRDAEQGRLPGNGHIHARPPTPEQAQAG
                     EKAKAARASAEAQIKDLSKDYPIFAEDDLPVDKRLDKTALAQASESQLAGVLQSHLAS
                     RAAVVAEARGQLEGKHELIYKMDKLMPTFYAEMDIQPGSIHDQIIQDKIHDDAIAKLV
                     GGILLAIVAIA"
     gene            complement(2825..3730)
                     /locus_tag="VF516_04900"
     CDS             complement(2825..3730)
                     /locus_tag="VF516_04900"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX8107042.1"
                     /translation="MPSRNGPCPCGSTLKYKKCCLAKDEAAQHALAPPPRPAPRISHL
                     MPTGPAKDRHLLVTMTNDPFQPVRLYYAVVDRALVLEKLAALRCVVEAPSERCWQWLF
                     HAEAASLRFPGGGYDDVPENKRPIVLGRIRFPEHGTMTLETNSIERAVAGARFFAPRL
                     GPAVVAMRCRLVNRCFAAHEGQPDQLMATLDQDVTVIDPRVAEAALKRRLAGVRSTQD
                     AERAAAESLEQRLKSKDDVPMVEDFPLAPEEETPDFTHLATALQFRFARALEHWRGNT
                     HLTLTAIIVRTVEQSMRDHAGREGS"
     gene            3951..>4170
                     /locus_tag="VF516_04905"
     CDS             3951..>4170
                     /locus_tag="VF516_04905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107043.1"
                     /translation="MSTCTHCGQRKGKRSCPALAGAICSRCCGQHRLVEIACPTDCVH
                     LGGLAVVRDPSRAVPFTRTDHLSAWDKLQ"
BASE COUNT          539 a         1408 c         1487 g          736 t
ORIGIN      
        1 gcgatggcga cgatcgcgag caggatgcca ccgacgagct tggcgatcgc atcgtcgtgg
       61 atcttgtcct ggatgatctg gtcgtgaatg gacccgggct ggatgtccat ctccgcgtag
      121 aaggtgggca tgagcttgtc catcttgtag atgagctcgt gcttgccttc gagctggccg
      181 cgggcctcgg cgacgacggc cgcacggctt gcgagatggg actgcagcac accggcgagc
      241 tggctctcgc tggcctgagc gagcgcggtc ttgtcgagtc gcttgtcgac cggcaggtcg
      301 tcctcggcga agatcggata gtccttcgag aggtccttga tctgcgcctc ggcgctggcc
      361 ctggcggcct tcgccttctc gccggcctgg gcttgctccg gcgtcggcgg ccgggcatgg
      421 atgtgcccgt tgccggggag ccgaccctgc tcggcgtccc ggttcgcttg cgcggcgtag
      481 tcgttccaga tcctcgcgtt ctggtcgaac tcctggaact gtgtgcggaa gccgccgagc
      541 ctggcatgca gctccttgac cacggcggca tcctggtagc gctgcgattc ctggtaaagc
      601 ttgccggcgt atctggcgag gacgtcgagg gtgatgcgga cagcgccgtc ctcgaacgcc
      661 tgctcgaacc gtgcgatgta cgccgcgaac tcgtcgatcg atgggaagcc gtagcgcgcc
      721 agctgctgct cgagctgttc gcgcatgccc ggtggtgcgg cctcgatcgg cgtggtgcgg
      781 tactcgcgat agagccggta cacctcctcg aggccgagca gcttgttctg caccgtcgtc
      841 cggtccgcgt cggcctgctc tcgcgcagca agctcgccgc gatagccgtc gatcgcggcc
      901 tcgaaggttg tgaggtccgc ggtgctgccg gtgatcttgc tcgtgtagtc cgcagcggcg
      961 ccggggggca gggcttcgat cttcttcgcg atgcgaaaca gctgatcgta gtccgcggga
     1021 gtcaggtccc ggccctggat gctgatgcgg atcagtgcct tgaccttgtc cggcaggttg
     1081 gcgaggtact gatgctggaa caggacctcg tcgcggatcg accgccacag ctcggccttg
     1141 ccggattcgt tcggctggat ctgggtcccg ggcgcggtgc cgctcgcggc gtggtacctg
     1201 cgatcgatct cggcgagcag ggcggccttc tggccggaag caggctgcgc ctggaagtcc
     1261 ttggcgcgcg catccgcgcc ggcgacgttg ccggtcgcgt cggtgtcgat gcccctggcc
     1321 gcgctggcgg cgccgcgcac gcgccggacg agcgatgcct cgaacagcac ctcgacgggg
     1381 ctgtcggcgg gcgtgtaggc gtccttgacg ttgtgccact gcgagggaat cgcggtggtg
     1441 ccgaacacct ggaggttcgc ggcggcctcg aactcctcgc gggtcatcgg ctttccgacg
     1501 gcgatccgga acggaatgaa ctccttcttg cccttcgcgc cgtgctgcgg ctggccggcc
     1561 ggcgccttct ggatcggcgg cgcagatccc gcacctacgt ccagcgcagc ggcggcttcg
     1621 ggaccggcga gctcctcgac gcgaggccgg gcgtccggcg gcgcggcgtc caccaggtct
     1681 gcatcggcct ttgcctgcct gggagcgtgg tcgccggctg cggtgggcgc gctcgcgggc
     1741 tgtggcccgg cacccgcctg cggggcagcc ccttgcgccg gcgtcccgcg cgccgccttc
     1801 gcacccagat cgtctgcttc gcgctcgagc gctgggtcgt cgttggccgg cgcgccatcg
     1861 atctgcgcgg tgaccggcac gcggccctcg gcctgctgct tgacgtgcgt gagctcgtgg
     1921 ccgatcagct ccaggccctg cgggctgatc gggtcaaacc gtcccggcgc gaagaagatg
     1981 tcggcgccgc gcgtgaacgc ctgcgcgccg tttgcctgtg cgtaggcgct ctggtggacg
     2041 cgcaccgcgg cgaagctgac gccgagcgcg gcctccatgc gcgcccgcac gtcgggcggc
     2101 agcggcgtcc caccttctcc gacggggggc ggtccagcgg catctgctgc gccggccccc
     2161 gtcgccttgc gctgtgcggg cccggcgccg agagagaatg cttcgagctc cgcgtggacg
     2221 cgcgtttgct ttccgggatc ctgcgcagcg cccctggccc cctcgagctc cgcggccacg
     2281 agcgactgct tcccggcccc ctgcgcgaga tcacccggct gcgtcgccga gcgcgcaccg
     2341 ggatcttggc gtcgatcgaa ccccatcccc ggacgcgagg ctagggaacg tggcacaacg
     2401 cgtcaagcgc gtacgtgcac ggttcgaggt ttccatgcca tgccggcgcc gctcgccccc
     2461 ggcgggagca gccgcaccga cggtccagcg cggaacgagg atcgcgtcgt tgacccatca
     2521 cgcgtcatcc tacggtccgc cctcgatgga tgcggcgggt gcccggtcga tatgacgggt
     2581 cggttgtcat tttcgcaaaa accgtcacga ctccatgtac gccgatggac gcgcggggcc
     2641 acatcaggcc ggcaaggacg tcgtcgaggg gccggctgtg gtcgtgtcgc tgaccaggcg
     2701 ggtcggccat ggcgggcatc cttccggcaa ggtgcgcgaa gaccgttgtg cgcgttgata
     2761 gcccatctgc ccggagtggc cttgccggtg cccggcggcc cgagaagacg atgatgaggg
     2821 atcgtcacga cccctcgcga ccggcgtgat ctcgcatgct ctgctcgacc gtgcggacga
     2881 tgatcgccgt caaggtgagg tgtgtgttgc ctcgccaatg ctcgagcgct cgggcgaagc
     2941 gaaactgcag cgcggtcgcc aggtgggtga aatcgggcgt ctcctcctcg ggagccagcg
     3001 ggaagtcctc gaccatcggg acgtcgtcct tgctcttgag ccgctgctcg aggctctcgg
     3061 cagccgcgcg ctccgcgtcc tgcgtggacc gcacgcccgc gagccggcgc ttcagggcgg
     3121 cctcggcaac gcgcgggtcg atcacggtga catcctggtc gagcgtggcc atcagctggt
     3181 ccggctggcc ttcgtgtgcg gcaaagcatc ggttcaccaa tcggcaccgc atggccacga
     3241 ccgcgggccc gagccgggga gcgaagaacc tggctcccgc gaccgctcgc tcgatcgagt
     3301 tggtctccag cgtcatggtt ccgtgttccg ggaagcggat gcgcccgagg acgatgggcc
     3361 gcttgttctc cggtacatcg tcatagccgc ctcccgggaa tcgcagcgac gcggcctcgg
     3421 cgtgaaacag ccactgccag cagcgctcgg agggagcctc gaccacgcag cggagcgccg
     3481 cgagcttttc gagcaccagg gcgcggtcca cgacggcata gtagagccgc accggctgga
     3541 acgggtcgtt ggtcatcgtc acgaggaggt gtcggtcctt ggcgggcccg gtcggcatga
     3601 gatggctgat ccgcggcgcc ggccgtggtg gcggcgcgag cgcatgctgt gctgcttcgt
     3661 ccttcgcgag gcagcatttc ttgtacttga gggtcgagcc acaggggcag ggtccgttcc
     3721 ggctgggcac gctccgatga tagccgccgg gtctgacgtc gcggggaacg atgacgttgc
     3781 gcgcaacgat cctcgatgga aacgaccagt tcccgcatct ggcagcgccg ccgcttcgcc
     3841 gtgtcgcgag ggctcgccgc cgtccaccag ttctgcggtc accgagccgt tcgcacggtg
     3901 agctggcatg ggacctggca ccggggatcg ccctgaggta cgtacgctcg gtgtcgacgt
     3961 gcactcactg tggtcagcgc aaaggcaagc gcagctgtcc ggcgctcgcc ggtgcgatct
     4021 gctcgcgctg ctgtggccag caccgactcg tcgagatcgc ttgcccgacc gactgcgtcc
     4081 atctcggcgg cctggcggtc gtccgcgatc cctcccgcgc ggttccgttc acccgcacgg
     4141 accacctctc cgcgtgggac aagctgcaag
//
LOCUS       DASVOR010000223         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_89883,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000223 DASVOR010000000
VERSION     DASVOR010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89883"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1663
                     /locus_tag="VF516_04910"
     CDS             <1..>1663
                     /locus_tag="VF516_04910"
                     /inference="COORDINATES: protein motif:HMM:NF018757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEX8107044.1"
                     /translation="GANAWRRALMIGLRAGAAVTALVVFLEPAVELRQVAREPNRIAV
                     LIDDSKSMALGEDPRGPSRIERARRLLAASGDALAAWERDHKIDYYTFAETVSATTLA
                     GLRKDPAQGKATLIRKALEYIRGRYEGHDLAGIVLISDGAATGGFDEDSGDGAVRDFL
                     RSLDTRVHTVWAARRGLRDVAVAKVMADEFAFVRTVVRIDAVIRTTGLPARQVPVTLS
                     TDGQPLRQKLVELPAGDHDVTVTFEVTPPRVGRYVYEVSVPVAPGEAVTTNNARSFVV
                     RVIRDKIRVLQVAGQPSWDVRALRQMLKTNPNVDLISFFILRTQDSISLVPNDEMSLI
                     PFPTRELFEQQLPSFDLIVLQDFEYLPYNIGDYLENIRSYVEGGGGLAMLGGAASFTS
                     GGYYGTPVAAALPVELYGPFDAGPILDTQKFVPQLTEAGLVHPVTSLRYSPDDNARVW
                     KQLPLLEGVNLIAGAKPDATVLAVHPRLKTRSGKPMPVIVAGEYGKGRSLAVTTDTLW
                     RWGFVAAARPGDDGRQYTKFWENSMRWLIQDPDLRNLHVDSDAVEY"
BASE COUNT          237 a          633 c          564 g          229 t
ORIGIN      
        1 ggcgcgaacg cgtggcggcg cgcgctgatg atcggcctgc gcgccggcgc cgcggtcacc
       61 gcgctggtcg tgttcctcga gcccgcggtc gagctccgcc aggtcgcgcg cgagcccaac
      121 cggatcgcgg tgctgatcga cgactcgaag tcgatggcgc tcggcgagga cccgcgcggc
      181 ccgtcgcgga tcgagcgcgc ccgccgcctg ctcgccgcgt ccggcgacgc gctcgccgcg
      241 tgggagcgcg accacaagat cgactactac acgttcgccg agaccgtcag cgcgaccacg
      301 ctcgccggcc tccgcaagga cccggcccag ggcaaggcga cgctgatccg caaggcgctc
      361 gagtacatcc gcggccgcta cgagggccac gacctcgccg gcatcgtgct gatctccgac
      421 ggcgcggcga ccggcgggtt cgacgaggac tccggcgacg gcgcggtgcg cgacttcctg
      481 cgctcgctcg acacccgggt ccacaccgtg tgggcggcgc gccgcgggct gcgcgacgtc
      541 gcggtcgcca aggtcatggc cgacgagttc gcgttcgtgc gcaccgtggt ccggatcgac
      601 gcggtgatcc gcaccaccgg cctgccggcg cgccaggtcc cggtcacgct gtcgaccgac
      661 ggccagccgc tgcgccagaa gctggtcgag ctgccggccg gcgatcacga cgtcaccgtg
      721 accttcgagg tcaccccgcc gcgggtcggc cgctacgtct acgaggtctc ggtgccggtc
      781 gcccccggcg aggccgtcac caccaacaac gcgcgcagct tcgtggtccg cgtgatccgc
      841 gacaagatcc gcgtgctgca ggtcgcaggc cagccgtcgt gggacgtccg cgcgctgcgc
      901 cagatgctca agaccaaccc caacgtcgac ctgatctcgt tcttcatcct gcgcacccag
      961 gacagcatct cgctggtgcc caacgacgag atgtcgctga tcccgttccc gacccgcgag
     1021 ctgttcgagc agcagctgcc gtcgttcgac ctcatcgtgc tgcaggactt cgagtacctg
     1081 ccgtacaaca tcggcgacta cctcgagaac atccgcagct acgtcgaggg cggcggcggc
     1141 ctggcgatgc tcggcggcgc ggcgtcgttc acctcgggcg gctactacgg cacgccggtc
     1201 gccgcggcgc tgccggtcga gctctacggc ccgttcgacg ccggcccgat cctcgacacc
     1261 cagaagttcg tgccccagct gaccgaggcc ggcctggtcc acccggtgac ctcgctgcgg
     1321 tactcgcccg acgacaacgc ccgggtgtgg aagcagctgc cgctgctcga gggcgtcaac
     1381 ctgatcgccg gggccaagcc cgacgccacg gtgctcgccg tccacccccg gctcaagacc
     1441 cggtccggca agccgatgcc ggtgatcgtc gccggcgagt acggcaaggg ccgctcgctc
     1501 gcggtcacca ccgacacgct gtggcggtgg ggcttcgtcg ccgcggcgcg cccgggcgac
     1561 gacggccgcc agtacaccaa gttctgggag aactcgatgc gctggctgat ccaggacccc
     1621 gacctgcgca acctccacgt cgacagcgac gcggtcgagt acg
//
LOCUS       DASVOR010000224         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_469785,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000224 DASVOR010000000
VERSION     DASVOR010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469785"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VF516_04915"
     CDS             <1..569
                     /locus_tag="VF516_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX8107045.1"
                     /translation="CERSAQCPETQQCVLGSCSLHAPAPADAPAPPADARTDAAIDAM
                     PLPCSTSGLSCAGGTATLFLCGVNCWVRCSASVGRETARAACEGWMGALGEIDNQTDQ
                     DCVNMHVGNTTIWVGMIQNNDPANTPSTGWTWNGIHPVTYTHWAAGQPDDGGGGEQGR
                     EQCMTLRNDGTWADDPCTNSRGFFCERP"
     gene            670..846
                     /locus_tag="VF516_04920"
     CDS             670..846
                     /locus_tag="VF516_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107046.1"
                     /translation="MDVLDMCSGAPVCMAARGVPRGTVCTYGTTTFVPEPDYTCEWTG
                     TACVRTPKPPKAPP"
     gene            843..1376
                     /locus_tag="VF516_04925"
     CDS             843..1376
                     /locus_tag="VF516_04925"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8107047.1"
                     /translation="MTRTVAEAVSDLSSPDGIVRDQALGLLVSYGTRATAALLPLLDD
                     DALRPRAARALGYIADPTTADRLAALLDDADPFVRSHAAYGLHQMKDPRALEALIQTI
                     DDFPTTTMFPATQSTGALRTLGAAVLPRVADLLTAPSADTRARARLVILQIASGLPPD
                     EAAAWRARVAATEGQSA"
     gene            1408..>1731
                     /locus_tag="VF516_04930"
     CDS             1408..>1731
                     /locus_tag="VF516_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8107048.1"
                     /translation="MTDQPSAHARLARFPITQKWPARHPERLQLYGLPTPNGAKVSIM
                     LEEIGLPYEAHLVSFDTQDQMSPEFGSLNPYNKIPAILDPDGPGGEPLPLFESGAILI
                     YLAEKT"
BASE COUNT          257 a          647 c          601 g          226 t
ORIGIN      
        1 cgtgcgagcg ctcggcgcag tgtccggaga cccagcagtg tgtgctcggc agctgctcgc
       61 tgcatgcgcc ggccccggcg gacgcgccgg ctccgccggc cgatgcgcgg accgacgcgg
      121 cgatcgacgc gatgccgctg ccgtgcagca cctccgggct gagctgcgcc ggggggacgg
      181 cgaccctgtt cctgtgcggc gtgaactgct gggtgcggtg ctccgcctcc gtcgggcgcg
      241 agacggcgcg cgcggcgtgc gaaggctgga tgggcgcgct cggcgagatc gacaaccaga
      301 ccgatcagga ctgcgtcaac atgcacgtgg ggaacaccac gatctgggtc gggatgatcc
      361 agaacaacga cccggcgaac acgccgagca ccggatggac ctggaacggc atccacccgg
      421 tgacgtacac ccactgggcg gcgggtcagc cggacgacgg gggcggtggt gagcagggca
      481 gggagcagtg catgaccctg cggaacgacg ggacgtgggc cgacgacccc tgcaccaatt
      541 cgaggggctt cttctgcgag cggccgtgac cgcgcgccgg atgcaatcgg ccctgcgagc
      601 cggtcagatg caaggccgct gcatgtatgg cgaggctgcg ccgaccgcgg cctcgcgccc
      661 ggcgatctgg tggacgtgct cgacatgtgc tccggcgcgc cggtgtgcat ggcggcccgc
      721 ggcgtgccca ggggcacggt gtgtacgtac ggcacgacca ccttcgtccc cgagccggac
      781 tacacttgcg agtggaccgg cacggcctgc gtccgcacac ccaagccgcc gaaggcccca
      841 ccatgacccg caccgtcgcc gaagccgtct cggacctgtc gtcgcccgac ggcatcgtcc
      901 gcgatcaggc gctcgggctc ctggtctcgt acgggacgcg cgcgaccgcg gcgctgctgc
      961 cgctgctgga cgacgacgcg ctccgccccc gcgcggcgcg cgcgctgggc tacatcgccg
     1021 accccaccac cgccgatcgc ctcgccgcgc tgctcgacga cgccgacccg ttcgtccgct
     1081 cgcacgcggc ctacggcctg caccagatga aggatccgcg tgcgctcgag gcgctgatcc
     1141 agaccatcga cgacttcccc accacgacca tgttcccggc cacgcagtcg accggcgcgc
     1201 tgcgcaccct gggcgcggcg gtcctgccgc gggtcgccga cctcttgacc gcgccgtcgg
     1261 ccgacacccg ggcccgcgcg cggctggtga tcctgcagat cgccagtggc ctgccgcccg
     1321 acgaggccgc cgcctggcgc gcgcgtgtgg ccgccaccga agggcagagc gcatgacacc
     1381 gccccgcggg atcggctaag catcggcatg accgatcagc cgtccgccca cgcgcgcctg
     1441 gcgcgctttc cgatcaccca gaagtggccc gcgcggcacc ccgagcggct gcagctctac
     1501 ggcctgccaa cacccaatgg tgcaaaggtg tcgatcatgc tcgaggagat cggcctgccc
     1561 tacgaggccc acctggtgag cttcgacacg caggaccaga tgtcgcccga gttcgggtcg
     1621 ttgaacccgt acaacaagat cccggcgatc ctcgatcccg acgggcccgg cggcgagccg
     1681 ctgccgctgt tcgagtccgg cgcgatcctg atctacctgg ccgagaagac c
//
LOCUS       DASVOR010000225         7427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_2236,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000225 DASVOR010000000
VERSION     DASVOR010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7427
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2236"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VF516_04935"
     CDS             complement(<1..1194)
                     /locus_tag="VF516_04935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX8107049.1"
                     /translation="MFQPAHRWFEEQVDRTPDAPAVVFDGQMFTYHELETRSNRLAHR
                     LLRVTSARDTCVGVVLRRGVDHLVALLALHKAGLTLVPVSSVSSNEPQERMAYIVENA
                     RVQLILTHRDLGNPWPHIEHLFFEDQADVPVSRPPVSVNPDDLAYVIHTSGSTGRPKG
                     CAMAHRGLANLVAWQLDYSRGTSEQRTLQFNGLSFDLCFQEIFPTWAVGGCLVVMPES
                     ARREPSALLDLMSRERVSRMILPFVVLRQLADAAELTNRTLPDLREVYVGGEALQLTP
                     AIRALFRRNPEAALINVYGPTEAHIVTAYKVPGSPDDWPAVAPIGTPIRGTRIYIVNQ
                     AGQKVPPGLPGEVMIGGDAIARGYLHNPRETAERFIPDRFANQPGARVYRTGDLARRL
                     PSGVLE"
     gene            1327..2163
                     /locus_tag="VF516_04940"
     CDS             1327..2163
                     /locus_tag="VF516_04940"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEX8107050.1"
                     /translation="MKLRLGALDELNARPLWFSLGTDPDVELIRDTRGGTTELLERDR
                     VDVSVVSVVKAAEEGWHRVPGIGIAASRTLGSVLLLSDVKPYEASVIYAAQDSRTSTM
                     LLLALLRRMGRNPSIIRLPAAEARARAKLDRGSAALLIGDEAMKARSSFTASWDLLEA
                     WNQRTGLPMVFGVWASRRPITADGSQRLIDAAAQGERQYAQIADRMADEGWITRDEAA
                     AYLHRLHYTLKPADEIAIELLLTILQSYELIRLPAGATTLERTHPDVLSWNQNDDAVH
                     HR"
     gene            2138..3388
                     /locus_tag="VF516_04945"
     CDS             2138..3388
                     /locus_tag="VF516_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX8107051.1"
                     /translation="MTTPFIIDDRELGSFKVNRRVFTDPEILRQERVKIFERTWIYAA
                     HESELKATGAFCARTVAGRPVIVVRGDDGNVRVFLNTCTHRGVPVCRTASGCAPTFRC
                     PYHGWTFSNQGRLIGLPQADAYSDAFNRDDLALASPPRVEIYRGFIFISFNPDIEPLV
                     AYLKDAKDYLDLVCDQSESGMEVIAGTHFYCTRANWKLVIENNIDLYHVATVHKRYFD
                     YAISAGGNSNTLRRIGRGLDLHNGHAAVESTPPAGGRLVAHWGPPFPESMRSDIELRR
                     RRIEERFGRERADQMTQYNRNLILFPNLIINDAISVVIRSSFPISEDYVEITAWALAG
                     ADESAEDRRMRIDSFLTFLGPGGFGTPDDNELLESCQRAFANKEVVWSDVSKGAKREH
                     PRHDDELQIRAFWRRWNELMTVGG"
     gene            3381..3878
                     /locus_tag="VF516_04950"
     CDS             3381..3878
                     /locus_tag="VF516_04950"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX8107052.1"
                     /translation="MGKTVLRQEVEDFLFAEAALLDEWRLDEWLALLTDDVTYIVPAT
                     DARDADPRTTLSIISDDAKRLRARAEQLLGKHAWAENPPSRTRRLITNVRIRRAVTTT
                     AGTTVEVTANFAVYRFRGDVAETFVGRYEYTLVRRADGLKIQIRKAILDLEALRPHGK
                     LSIIL"
     gene            3875..5209
                     /locus_tag="VF516_04955"
     CDS             3875..5209
                     /locus_tag="VF516_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8107053.1"
                     /translation="MSTKTITEPARDLPVVDEADVLVLGGGPSGISAAVAAARMGART
                     ILVERYGFLGGMGTAAMVTNFCGLFADVGGVVTQVVHGIGDDILARIRALGGLNRPQS
                     KGATTVISYDNAAYKHAADQLLIEAGVRLRLHTLAVGAVMDRSRIVGLIVESKSGRAA
                     LCAHTFIDCSGDADLAAWAGAPYEKGDSSGYLAYPTMMFRMGGVDTEVAEREGLPLIR
                     RHAAEARARGESSFMRASPIAAPQAHPGEWRMNMTQVSLAGRSLDGTDVEHMTRGEIL
                     GREQVFQADRYLRDQVPGFKHSYVLEIAPQLGIRETRRIIGQYVLTASDVAEARDFPD
                     AIGCSGWPMEKHVLGGVEWTFIGGRGYHQVPYRALVPRGVDNLLVAGRCFSATQDAQA
                     SARVSGPCFVMGQAAGTASALSVQRGCDVAAIEADRLQASLRAQNVFLGHER"
     gene            5206..6375
                     /locus_tag="VF516_04960"
     CDS             5206..6375
                     /locus_tag="VF516_04960"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8107054.1"
                     /translation="MNRSPRHVVSFDGLRGLGALCVLCTHYALHLFQDAPGYPSVAGT
                     MIAVDHTPLHVIFVGYPWLNMFFVLSGFALSAMVSGPGNTPRTYLPYAINRVFRIYPA
                     YFFVVLATFLLQHFLYKGPISAFGDWFNSCWTTPPTRKDLLDHAIMIGRFKTNRFVFV
                     IWSLVHEMRISLLFPLLYAVVARLGVRGNLYVGIGLLALDFTWATLADNDYLSPNNYY
                     FTLHYMLFFWAGSVIFRFRGALKQWWGERPNGQIAILGVVGFILFTYGDSVDKFLTPD
                     AMRTVVIADWLSGPGVVLLIIFALYSAPLSGFLSTQTMRFFGAISYSLYLLHGVVLIA
                     ALQAFYGVVPLGILLPSCVAVSVLLSFMSLTIVEKPFMSFGKRIASSVARKMTRS"
     gene            complement(6398..>7427)
                     /locus_tag="VF516_04965"
     CDS             complement(6398..>7427)
                     /locus_tag="VF516_04965"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX8107055.1"
                     /translation="VGISFFTFVNVAYVVDVYRRTIGAQHAVFDCLAPLTFFPHLLSG
                     PIVRMPQLVPQFERLAGIQASTYRFGLLLLFLGLAKKTLAGLLDPVAASVFADPPSSL
                     AAWTSVLAYAAQIYADFCGYTDMALGAALLIGIELPHNFELPYIATSPIDFWRRWHKT
                     LSSWIHDYVFMPLARYSRRTYASLLITWLLVGVWHGPQWTFVAYGLYHGLAIIATRFI
                     STRAPDAWHDQAARWPLATLCRAITFLIVAMSFILFRAPDLGAAAGLARSLFVADAES
                     VFSYAACLTLALVTVALVVPHLVDAVIWKRRDAVQRGLVFWPASVLAVTFTLVFGTNA
                     GSFIYFKF"
BASE COUNT         1341 a         2362 c         2404 g         1320 t
ORIGIN      
        1 ctccaggaca ccgctgggga gccggcgcgc caggtcgccg gtccgataga cccgggctcc
       61 gggttggttg gcgaaccgat ccggaatgaa ccgctccgcg gtctcccgcg ggttgtggag
      121 atacccccgc gcgatggcgt cgcccccgat catgacctca cccggcaagc ctggcggcac
      181 cttctgcccg gcctggttga cgatgtagat cctcgtcccg cggatcggcg tcccgatcgg
      241 cgcaactgca ggccagtcgt ccggggatcc aggaaccttg taggccgtga cgatgtgggc
      301 ctccgtcggg ccatagacgt tgatcagcgc agcctcgggg ttgcgccgga acagcgcgcg
      361 gatcgctggc gtgagctgca gtgcttcgcc accgacgtat acctcccgca gatcgggcaa
      421 cgtgcgattg gtcagctcgg ccgcgtcggc cagctgtcgc aagacgacga acggcaggat
      481 catgcgcgac acacgctcgc gactcatcag gtcgagcagc gcgctgggct cgcggcgcgc
      541 cgactctggc atgaccacga ggcaaccacc gaccgcccag gtcggaaaga tctcctggaa
      601 acagagatcg aagctgagcc cattgaactg caatgtccgc tgctcggacg tgccgcgcga
      661 gtagtcgagt tgccaggcga ccaggttggc caggccccgg tgcgccatcg cgcagccctt
      721 gggtcggccg gtcgaccccg acgtatggat gacgtaggcg agatcatcgg gattcaccga
      781 aaccgggggc cgagatacgg gcacgtccgc ctggtcttcg aagaagaggt gctcgatatg
      841 cggccacggg ttgccgaggt cgcggtgcgt caggatcagc tggacccgcg cgttctcgac
      901 gatgtacgcc atgcgctcct gcggctcgtt cgacgagacg ctcgataccg gcaccagcgt
      961 caagccggcc ttgtggagcg cgagcaacgc gacgaggtga tccacgccac gacggagcac
     1021 cacgccgaca cacgtgtcgc gagcgctggt aacgcgcagc aggcgatgcg cgaggcgatt
     1081 ggaccgggtc tctagctcgt ggtacgtgaa catctggccg tcgaacacga ccgccggagc
     1141 gtctggcgtt cgatctacct gttcctcgaa ccatcgatgc gcgggttgga acatcaccgt
     1201 gatcgtacac gatgaggggc tccgcagagg cgtgctcggg ctggaatcga actgattgac
     1261 ggcctcgccc ttgtacgcta gcttcactcc ccgtcgggcc cttgtcgcgg ctcggaggcc
     1321 gtctatatga agcttcgact gggcgcgctc gatgagctca atgctcgccc gctgtggttc
     1381 tcgctcggga cggacccgga cgtcgagcta atcagggaca cgcgaggggg cacgaccgag
     1441 ctcctcgaac gagatcgcgt cgacgtatcg gtcgtgtccg tcgtgaaggc ggccgaggag
     1501 ggctggcatc gcgtcccggg catcggaatc gcggcatcga gaaccctggg cagcgtcctg
     1561 ctgctctccg acgtgaagcc gtacgaggcg tccgtcatct atgccgcaca ggattcgcgc
     1621 acgtccacca tgcttctcct ggcgctgttg cgtcgcatgg gacgcaatcc atccatcatc
     1681 aggctgccgg cggccgaagc gcgagcgaga gcgaagctgg accgcggcag cgcggcgctg
     1741 ctcatcggtg atgaggcgat gaaggctcgc tcgtcgttca ccgcgtcgtg ggatctgctg
     1801 gaggcctgga accagcgtac cgggctcccg atggtgtttg gcgtctgggc gagccgtcgg
     1861 ccgatcacgg ccgacggatc ccagcgcctg atcgacgccg ccgcgcaggg cgagcggcag
     1921 tacgctcaga tcgccgatcg gatggcggac gaggggtgga tcactcgaga cgaggcagcg
     1981 gcatatctcc accgtctcca ttacaccttg aagcccgccg acgagatcgc gatcgagctg
     2041 ctgctgacca tcctgcaatc gtatgagttg atcaggttac ctgccggtgc caccacactg
     2101 gagagaacac accccgacgt gttgtcctgg aaccagaatg acgacgccgt tcatcatcga
     2161 tgacagagag ctcggctcct tcaaggtcaa tcgacgggtc ttcaccgacc ccgagatcct
     2221 ccgccaggag cgagtgaaga tcttcgagcg cacctggatc tacgccgcac acgaatcgga
     2281 gctcaaggca accggtgcgt tctgcgctcg gacggtcgca ggtcggcccg tgatcgtggt
     2341 gagaggtgac gacggcaacg tccgcgtctt cctcaacacg tgcacccatc ggggcgttcc
     2401 cgtctgtcgc actgcctcgg ggtgcgcgcc cacgtttcgc tgcccgtatc acggctggac
     2461 gttcagcaac cagggccgcc tcatcggtct gccacaagca gacgcctaca gtgacgcatt
     2521 caaccgggac gacctcgcgc tcgccagccc gccgcgggtc gagatctatc gaggattcat
     2581 ttttatcagc ttcaatcccg atatcgagcc gctggtcgcg tatctcaagg acgccaagga
     2641 ctacctcgac ctggtctgcg atcagtccga gagcggcatg gaggtgatcg ccgggacgca
     2701 cttctactgt acacgcgcaa actggaagct cgtcatcgag aacaatatcg acctgtacca
     2761 tgtcgccacg gtgcacaagc ggtacttcga ctacgccatc agcgccgggg gtaatagcaa
     2821 cacgctgcgg cgcatcggca ggggcctcga tctgcacaac ggccatgccg ccgtcgagag
     2881 caccccgccg gcagggggac gtctggtcgc gcactgggga ccaccgttcc ccgagtcgat
     2941 gcggagcgac atcgagctgc gacgccgccg gatcgaggag cggttcggcc gcgagcgggc
     3001 ggatcagatg acgcagtaca accgcaacct gatactcttt ccgaacctca tcatcaacga
     3061 tgcgatctcc gtggtcattc gatcctcctt cccgatctcc gaagactacg tggagatcac
     3121 cgcgtgggcg ctcgccggtg cggacgagag cgccgaggat cgccggatgc ggatcgacag
     3181 ctttctcacg ttcctcgggc ccggcggatt cggcacaccc gatgacaacg aactcctcga
     3241 atcgtgccag cgcgccttcg cgaacaagga ggtcgtgtgg tcagacgttt cgaagggggc
     3301 caagcgtgaa catccgcgac atgacgacga gctgcagatc cgcgcgttct ggagaaggtg
     3361 gaacgagctc atgaccgtgg gtgggtaaga ccgtgctacg gcaggaggtc gaggactttc
     3421 tgttcgccga agcagcgctg ctcgatgaat ggcgcctcga cgaatggctc gcgctgttga
     3481 cggatgacgt gacgtacatc gtgcccgcca cggacgcacg agatgccgat ccgcgcacca
     3541 ccctgtcgat catctcggac gacgcgaagc gcctgcgggc tcgagccgaa cagctgctcg
     3601 gcaaacatgc ctgggccgag aatccaccgt cgcgcacgcg ccggctgatc acgaacgtgc
     3661 gcattcgccg cgcggtcacc accaccgcgg gcaccaccgt cgaggtgacc gcgaactttg
     3721 ccgtctaccg ttttcgcggc gacgtcgcag agaccttcgt gggtcgatac gaatacacgc
     3781 tcgtccgacg cgcggacggt ctgaagatcc agatccggaa ggccatcctg gacctggaag
     3841 cactccgccc gcatggcaag ctgagcatca tcctttgagc acgaagacga tcacggagcc
     3901 ggcacgcgat ctaccggtcg tcgacgaggc cgacgtgctc gtcctcggtg gaggaccgtc
     3961 cgggatctcc gccgccgtgg cagcggcgcg catgggcgcg aggaccatcc tggtcgaacg
     4021 ctacgggttt cttggcggga tgggaaccgc cgcgatggtg accaacttct gcgggttgtt
     4081 cgccgacgtg ggcggcgtcg tcacgcaggt cgtccatgga atcggcgacg acatcctcgc
     4141 tcgtattcgt gcgctcggcg gattgaatcg gccgcagagc aaaggcgcga cgacggtcat
     4201 ctcgtacgac aatgcggcct acaagcatgc tgccgatcag ctgctcatcg aggccggcgt
     4261 tcgtctccgc ctgcatacgc tcgcggtggg cgcggtgatg gaccggtcac gtatcgtggg
     4321 cctgatcgtg gagagcaagt cgggccgtgc tgcgctgtgc gctcacacct tcatcgattg
     4381 ttccggggat gcggacctcg ccgcctgggc cggggccccg tacgagaagg gcgacagcag
     4441 cggatatctg gcgtatccga cgatgatgtt ccggatgggc ggcgtcgaca cggaggttgc
     4501 cgaacgagag ggattgccat tgatccgccg ccacgctgcc gaagcgaggg cccggggcga
     4561 gagcagcttc atgcgagcct ctccgatcgc cgctccgcag gcccacccag gcgagtggcg
     4621 catgaacatg acgcaggtga gtctggcggg ccgatcactc gacgggaccg acgtggagca
     4681 catgacacgc ggggagatct tggggcgcga acaggtgttc caggcggacc gctatctgcg
     4741 tgaccaggtt ccgggcttca agcacagcta cgtgctcgag atcgccccgc agctcggtat
     4801 ccgcgagacc cgacggatca tcggccagta tgtcttgacc gcctcggacg tcgcggaggc
     4861 ccgggacttt cctgatgcga tcggttgttc tggttggccg atggagaagc acgtcctcgg
     4921 cggcgtcgag tggacgttca tcggcggccg gggctatcac caggtcccct accgcgcgct
     4981 cgtgccgcgc ggcgtcgaca acctgctcgt ggcgggccgc tgtttctcgg cgacccagga
     5041 cgcgcaagcc tcggcgcgcg tcagcggtcc ctgcttcgtc atgggccagg cagcggggac
     5101 cgcgagcgcg ctcagcgtcc agcgcggatg tgacgttgca gcgatcgagg cggatcggct
     5161 acaggcatcg ttgcgggccc agaacgtctt tctcgggcac gaacgatgaa caggtccccg
     5221 cgccacgtcg tctcgttcga tggactccgg gggctcgggg cgctgtgcgt tctatgcact
     5281 cactacgccc tccatctgtt ccaggacgcg cctggttacc catccgtggc cggcacaatg
     5341 atcgcggtgg atcacacgcc attgcacgtc atcttcgtcg gctatccctg gctcaatatg
     5401 tttttcgtgc tcagcgggtt cgcgctgtca gcgatggtgt ccggtcccgg aaatacgccc
     5461 aggacgtatc tgccgtacgc gatcaatcgc gtgtttcgga tctaccccgc ttacttcttc
     5521 gtcgtgcttg ccacgttcct tctgcagcat ttcctttaca agggacccat ttctgccttt
     5581 ggtgactggt tcaatagctg ctggacgacg ccgcccacgc gaaaagattt gctagatcac
     5641 gcgatcatga tcggccggtt caagacgaac aggttcgtct tcgtgatctg gtcgctggtt
     5701 catgagatgc gaatttccct cctgtttccg ctcctctatg cagtcgtcgc caggctcggc
     5761 gtgagaggaa acctctacgt tggcatcggg ctgctcgcgc tcgatttcac gtgggccacg
     5821 ctggccgaca acgattacct gtcgcctaat aattattatt tcacactaca ctacatgctc
     5881 ttcttctggg caggcagcgt gatcttcagg tttcgtggag cgctcaaaca atggtggggg
     5941 gaacgtccca acgggcagat cgcgatcctg ggggtagttg gcttcatcct cttcacctac
     6001 ggcgacagcg tcgacaagtt cctgacgccg gacgccatgc gtaccgtggt catcgccgat
     6061 tggctgagcg gtcccggcgt cgtgctcctc atcatctttg cgctgtactc ggcaccacta
     6121 tctggattct tgtcgaccca gaccatgagg ttctttggcg cgatctccta cagcctttat
     6181 ctcctgcacg gggtcgtatt gatcgcggcc ctccaagcgt tctacggcgt cgtcccgctc
     6241 gggatcctgc tgccgtcctg tgtggcggta agcgtcttgc tctcgttcat gtccttgacc
     6301 atcgtcgaga agccgttcat gagcttcggc aagcggatcg cctcatccgt tgctcggaag
     6361 atgacgcgat cgtgagtcta gtgtctggtg agctacttta gaacttgaag tagatgaagc
     6421 tgccggcgtt cgtgccgaat acgagcgtga acgtcaccgc gagcacgctc gccggccaga
     6481 acaccaggcc gcgctggacg gcatcgcgac gcttccagat cacggcgtcc acgagatgtg
     6541 gcacgaccag cgccaccgtg acaagcgcga gcgtcaagca ggctgcgtag ctgaacacgg
     6601 actcggcgtc cgcgacgaac aacgacctcg cgaggccagc cgcagcgccg agatcgggcg
     6661 cgcgaaacaa gatgaagctc atcgcgacga tgaggaaggt gatcgcgcgg cacaacgtcg
     6721 cgagcggcca gcgcgccgcc tggtcgtgcc aggcatcggg agctcgcgtc gagatgaagc
     6781 gcgtcgcgat gatcgcgagg ccgtgataca gaccgtaggc aacgaacgtc cactgcggac
     6841 cgtgccacac gccgacgagg agccacgtga tcaagagcga tgcgtacgtc cgccgcgagt
     6901 agcgcgcgag cggcatgaac acgtagtcgt ggatccagga cgacagcgtc ttgtgccagc
     6961 gcctccagaa gtcgatcggg gaggtcgcga tgtatggaag ctcgaagttg tgcggcagct
     7021 cgatgccgat gagcaacgcc gcgccgagcg ccatgtcggt gtagccgcag aagtctgcat
     7081 agatctgggc ggcgtatgcg agcacgctgg tccatgccgc gagcgagctc ggcggatctg
     7141 cgaacaccga cgccgcgacc ggatcgagca gcccggcgag cgtcttcttc gcgaggccga
     7201 ggaacagcag caacagcccg aaccggtacg tgctcgcctg gatcccggcg aggcgctcga
     7261 actgcggcac cagctgcggc atgcggacga tcggccccga cagcaggtgc ggaaagaacg
     7321 tgagcggcgc gaggcagtcg aacacggcgt gctgcgcacc gatcgtccgc cggtacacgt
     7381 cgaccacgta ggcgacgttg acgaacgtga agaacgagat gccgacc
//
LOCUS       DASVOR010000226         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_206757,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000226 DASVOR010000000
VERSION     DASVOR010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206757"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF516_04970"
     CDS             complement(<1..401)
                     /locus_tag="VF516_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107056.1"
                     /translation="MSSRVTGAGLALFAVALLAVSLLTPAVLPAQLSLFAGHPTVAEH
                     TRTTQDVYVGLYSAELCNSGGDGTCKSGDATGGFRAFGYAELGLTGVLAAAALGLGLL
                     TLGRSERRKAAARIVWVTGGLAIACAAALVIL"
     gene            536..1165
                     /locus_tag="VF516_04975"
     CDS             536..1165
                     /locus_tag="VF516_04975"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEX8107057.1"
                     /translation="MRMILVGPPGAGKGTQAARLVDSYRIPHISSGDMLRAAVKEGTA
                     LGIEADRFMKAGKLVPDEVVIGMILERIARPDCAAGFMLDGFPRTAPQAEALDAAMRA
                     AGVELDAVVLIEVPDALLEERAVGRRSDPETGAIYHLKYNPPPPQIAPRLIHRKDDTI
                     EAVQTRIQKYHSETAPIIPFYRAKGILRQVDGVGDPDVITQRITAVLSS"
     gene            1172..>1501
                     /locus_tag="VF516_04980"
     CDS             1172..>1501
                     /locus_tag="VF516_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8107058.1"
                     /translation="MAGFDHKLIDAKWQAYWEREQTFRTPTDRTRPKYYVLDMFPYPS
                     GDGLHVGHPKGYTATDVVARAKRMMGFNVLHTMGWDSFGLPAERRAERTGEHPSVITA
                     RNIATFKR"
BASE COUNT          247 a          545 c          524 g          185 t
ORIGIN      
        1 aggatgacca gcgcggccgc gcaggcgatc gcgagcccgc cggtcaccca cacgatccgc
       61 gcggcggcct tgcggcgctc gctccggccc agcgtcagca ggccgagccc gagggcggcg
      121 gccgcgagca cgccggtcag cccgagctcg gcgtagccga acgcgcggaa cccgccggtg
      181 gcgtcgcccg acttgcacgt gccgtcgccg ccgctgttgc acagctcggc cgagtacagc
      241 cccacgtaga cgtcctgcgt cgtccgggta tgctcggcga ccgtcggatg cccggcgaac
      301 agcgagagct gcgcagggag caccgccgga gtgaggagcg acaccgcgag cagcgccacc
      361 gcgaacagcg cgagacccgc gccggtgacg cgagaggaca tgtccgaaaa ctaccacacg
      421 gcgcaccggg cccgggcgca cgcaggtcgc gcgatgtcgg gtgggacgtg ctgcggcatc
      481 gccgcctggc ccgggcaatt gcgcgccgtc ggtccgcatg cttggatgcc gatctatgcg
      541 catgattctc gtcgggcctc ccggcgccgg aaagggcaca caggccgcca ggctggtcga
      601 ctcctatcgc atcccgcaca tctccagcgg cgacatgctg cgcgccgcgg tcaaggaggg
      661 caccgccctg gggatcgagg ccgaccggtt catgaaggcg ggcaagctgg tgcccgacga
      721 ggtcgtgatc ggcatgatcc tggagcggat cgccaggccc gactgcgccg ccgggttcat
      781 gctcgacggc ttcccgcgca ccgcgccgca ggccgaggcg ctcgacgccg cgatgcgcgc
      841 cgccggggtc gagctcgacg ccgtggtgct gatcgaggtc cccgatgcgc tgctcgagga
      901 gcgcgcggtc ggccggcgca gcgacccgga gaccggcgcg atctaccacc tcaagtacaa
      961 cccgccgccg ccgcagatcg cgccgcgcct gatccatcgc aaggacgaca cgatcgaggc
     1021 ggtccagacc cggatccaga agtaccactc cgagaccgcg ccgatcatcc cgttctaccg
     1081 cgcgaagggc atcttgcggc aagtcgacgg cgtcggcgac ccggatgtca tcacccagcg
     1141 gatcaccgcg gtattgagca gctgaggggc catggcgggt ttcgatcaca agctcatcga
     1201 tgccaagtgg caggcgtact gggagcgcga gcagacgttc cggacgccga ccgatcgcac
     1261 gcggcccaag tactacgtgc tcgacatgtt cccctacccg tcgggcgacg gcctgcacgt
     1321 cggccacccc aagggctaca ccgcgaccga cgtggtcgcc cgcgccaagc ggatgatggg
     1381 gttcaacgtg ctgcacacga tggggtggga cagcttcggg ctgccggccg agcgccgcgc
     1441 cgagcgcacc ggcgagcacc cgtcggtgat caccgcgcgc aacatcgcca cgttcaagcg
     1501 c
//
LOCUS       DASVOR010000227         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_177586,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000227 DASVOR010000000
VERSION     DASVOR010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177586"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..>2610)
                     /locus_tag="VF516_04985"
     CDS             complement(109..>2610)
                     /locus_tag="VF516_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8107059.1"
                     /translation="REWVRLGRGKSALMDEIELREVQRWLDGAAGLGLRPSDDLLALI
                     ARSERAVASRARRYRFMLARLIVSTIALAVMLVITWVQYRAAQRAHEHELAIEKQRQL
                     DNERLLGRASMEQGRELLLDGLVTHPMRALAYLVEARTLGIETPTLRMLFAQAARSTP
                     LVTLIGHAAAVNQAVFSPDGARVVTASDDRTARVWDARTGALITPPLVHQDAVAIAAF
                     SPDGKRVVTASKSREVRVWDATTGATLLSWLVPDGSIEAAMFSPDGAQVITVSGHTVL
                     AWDAASAAPIARLVPRDRIAATRVVTVGKERTARVWDARTGQPLTALLSHQSEITAVS
                     FSPDGTRVVTASDDRTARVWDVRTGEPVTNSLEHEGRVTSAAFNADGTRVVTASFDNT
                     ARVWDAQAGRPITAPLEHQGRIRSAAFSPDGTRVVTAGEDSCARIWDARTGALVSVVL
                     RTGDGGPAATTRPDRDDAAARARWITSGGQPVVMFDRASPAQEPAAPAAADDAPAGKP
                     HEPSLSAAFSPDGTRVVMEDGDAARVWDAATGAPVTTPLPHHGQVLAAAFSPDGARVV
                     TASVDGTARVWNAATGVPVTGPLPHHGPVLAAMFSPDGSRVVTASDDGTARIWDAVTG
                     KPAIALDHHAAVLAAAYSRDGRRVVTASDDDTAQIWDAVTGKPVTGPLSHRGRVTAAA
                     FSPDGSRVVTASDDSTARIWDAATGKPLTAPLEHQGGVTAAGFSTDGTRVVTASEDRT
                     ARVWDAVSGRPVTAPLQHRGRVFAAAFSPDGTRVVTASGSFHGRIWELPIDDGSLDDW
                     QQIARCSPFAIDGNVFGANPALRAVCEHRRRSEPR"
BASE COUNT          308 a         1031 c          918 g          353 t
ORIGIN      
        1 gcccggtcgg ggccgcggtg cgatcgagcg cgtcgccggc gaggccgtcg gcgagcgcgg
       61 gccggcggtc atcggcgtcg gcgagcaaca ggtgcagcgg cggcgccgtc atcgcggctc
      121 cgatcgccgg cgatgctcgc acaccgcgcg cagcgccggg ttggcgccga acacgttgcc
      181 gtcgatggcg aacggcgagc agcgcgcgat ctgctgccag tcgtcgagcg atccgtcgtc
      241 gatcggcagc tcccagatcc ggccgtggaa ggacccgctc gcggtgacca cccgggtgcc
      301 atccgggctg aacgccgcgg cgaacacccg gccgcgatgc tggaggggcg cagtcacggg
      361 cctgccgctc acggcgtccc acacccgcgc cgtgcggtcc tcgctcgcgg tcaccacgcg
      421 ggtgccgtcg gtgctgaacc cggcggccgt gacgccaccc tggtgctcga gcggcgcggt
      481 cagcggcttg ccggtcgccg cgtcccagat ccgcgccgtg ctgtcatcgc tcgcggtcac
      541 cacccgcgac ccgtcggggc tgaacgccgc ggccgtgacg cgaccgcgat gcgacagcgg
      601 cccggtcacc ggcttgccgg tcaccgcatc ccagatctgc gccgtgtcat cgtcgctcgc
      661 ggtcaccacc cgccggccat cccggctgta ggcggcggcg aggaccgcgg catgatggtc
      721 gagtgcgatc gccggcttgc cggtcaccgc gtcccagatc cgcgccgtgc cgtcgtcgct
      781 cgcggtgacc acccgcgacc cgtcggggct gaacatcgcg gcgaggaccg gcccgtgatg
      841 cggcagtggc ccggtcaccg ggaccccggt cgccgcgttc catacccgcg ccgtgccgtc
      901 gacactcgcg gtgaccaccc gcgccccgtc ggggctgaac gccgcggcga ggacctgccc
      961 gtgatgcggc agcggcgtgg tcaccggggc cccggtcgcc gcgtcccaca cgcgcgcggc
     1021 gtcgccatcc tccatgacca cgcgcgtgcc gtcgggactg aacgctgcgg acagcgacgg
     1081 ctcatgcggc ttgcccgcag gtgcgtcgtc ggccgcggcc ggcgcggcgg gctcctgcgc
     1141 cggactcgcg cggtcgaaca tgaccaccgg ctgcccgccg gacgtgatcc atcgcgcccg
     1201 cgccgcggcg tcgtcgcgat cgggacgcgt cgtcgccgcc gggccgccgt ccccggtgcg
     1261 gagcacgacc gacaccagcg ctccggtccg tgcgtcccag atccgcgcgc agctgtcctc
     1321 tcccgccgtg accacgcgcg taccgtccgg gctgaacgcg gccgaccgga tccgcccctg
     1381 gtgctccagc ggtgcggtga tcggtcggcc ggcctgggcg tcccagaccc gcgcggtgtt
     1441 gtcgaagctc gcggtgacca cccgtgtgcc atccgcgttg aacgcggcgc tcgtgacccg
     1501 gccctcgtgc tccagcgagt tggtcaccgg ctctccggtc ctgacatccc acacccgcgc
     1561 cgtccggtcg tcgctcgcgg tgaccacccg cgtcccatcc gggctgaacg atacggccgt
     1621 gatctcgctc tgatgcgaga gcagcgcggt cagcggctgt cccgtccggg cgtcccacac
     1681 ccgcgcggtc cgttccttgc ccacggtgac cacccgcgtg gccgcgatcc ggtcgcgcgg
     1741 aacgagccgc gcgatcggcg ctgcggaggc cgcgtcccac gccagcaccg tgtggccgct
     1801 caccgtgatg acctgcgcgc catccgggct gaacatcgca gcctcgatcg agccgtccgg
     1861 cacgagccac gacagcagcg tcgcgcctgt cgtggcgtcc cacacccgca cctcccggct
     1921 cttgctcgcg gtgaccaccc gcttgccgtc cgggctgaac gccgcgatcg cgacggcatc
     1981 ctgatgcacg agcggcgggg tgatcagcgc tccggtgcgg gcgtcccaca cccgcgcggt
     2041 ccggtcatcg ctcgcggtga ccacgcgcgc gccgtccggg ctgaacaccg cctggttgac
     2101 cgcggccgcg tggccgatca gcgtcaccag cggtgtgctc cgggccgcct gcgcgaacag
     2161 catgcggagc gtgggcgtct cgatccccag cgtccgggcc tcgaccaggt aggccagcgc
     2221 gcgcatcggg tgggtgacca gaccatcgag cagcagctcg cggccctgct ccatgctggc
     2281 cctgcccagc aggcgctcgt tgtcgagctg gcgctgcttc tcgatcgcga gctcgtgctc
     2341 gtgcgcgcgc tgcgccgccc ggtactgcac ccaggtgatc accagcatca cggccagcgc
     2401 gatcgtcgag acgatcagcc gggcgagcat gaagcggtac cgccgcgccc gggacgcgac
     2461 ggctcgctcg ctgcgcgcga tcagcgcgag caggtcgtcg ctcggccgca gcccgaggcc
     2521 cgccgcgccg tcgagccagc gctggacctc gcgcagctcg atctcgtcca tcagcgcgct
     2581 cttgccgcgg ccgagccgga cccactcgcg
//
LOCUS       DASVOR010000228         3821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_323639,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000228 DASVOR010000000
VERSION     DASVOR010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323639"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..542)
                     /locus_tag="VF516_04990"
     CDS             complement(360..542)
                     /locus_tag="VF516_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107060.1"
                     /translation="MNFSVTGSVSGTARWRIDASSREILGSERSIATASDDEIAMSSA
                     IEAILSAHRLASAERD"
     gene            701..>3821
                     /locus_tag="VF516_04995"
     CDS             701..>3821
                     /locus_tag="VF516_04995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107061.1"
                     /translation="MSWTSREEVARSLLERLFPRFASQSGLTAMERGRVWSLALAVEL
                     APLELAPLLLQIAIDPDLPDPDLEEGERCTVLCRLRRLGVAVPPSALRQLLEDAVGPA
                     PQMSVIWELLHHTDSLDESTLAVLDALPPEQRNSLFHDFADGDDVPCKAVRHALWRRW
                     AGDDIDLAIAAGPSYSGATETVAAWLNAAPASEDAMAALARFPSLAIEHPGAAEALVL
                     PPTLLLERLGVARLYTLVHQAVVLQGSEPRSPTATEFQDARRYEAALSVLRKIPEVRG
                     ELARLLDSVVLPPRVHLDLVRIALDIDRGATLTYLARYPNLWRDVARTLEESPRPGDR
                     AFAEAAARSTDPFTAYHAIGVLDQLGGPLPPLPVAVLPRVRMLGAHARRGDRGAVAAL
                     IEATSAPEVLVRAEAERWLGRLDDAREHLAIFERALADRERCMWRCPPRQPVAEQAAI
                     AFARAGGSRTLLLRTYFAVGSRCADTIEDLLCDRPPTLESESLPPFDPLDLPRPSWGR
                     KPAYGKPSVAYCVCCPHTYDDTENYNWLSWTSREEVARSLLEQLFPTATQSELTAIAP
                     AHKPLQRWRTLSLATALAPLTLAPLLLRIALDPDLRHGELCEVLSRLRELGVAVPPDT
                     LRPLLHRLLEGAVGPVGPHSEVTWTAELTATSELLQYVDSLDESTLAVLDVHPPAQRK
                     LLLRRWTEGDDVPCKAVRDALWRRWAGDDVDLAIAAGPSYPGTTETVAAWLSAAPESE
                     DTMDALARFPSLAVEHPGAAEALALAPTLLRERLGVAQLRELVHQAVVLQSVQERSIP
                     EAEFQRPRRYQAALSVLREVPEVRGEVARLLGSVVLTPRVHIDLVQIALGIDRGATLT
                     YVARYPDLWCEVARTLEESPQPGDRAFAEAAARSTDPGTGYHAIGVLDQLGGPLEPLP
                     LAALPQVRMLGARAQRGDRDAVAALIEATSAPEVAVRAEAVRWLGRLDDAREHLAIFE
                     RALSDRVRCGKFWPGYAVADQAAIALDRAGGSRTWLLRTYFSLPYAGGCASDTIVKLL
                     CDRPPISPRD"
BASE COUNT          555 a         1335 c         1318 g          613 t
ORIGIN      
        1 cgagaacgcg ccggtgcgga cgccgaccag cgcggtgccg ccggccgcca cggtcccggc
       61 gcacgcctgc ttgcacgcct cgaggttggc cggttggccg ccgcacccgc gatcaggcaa
      121 gcgggaatcg agatgatacg gctggcaagg tgctcgttca tcgtgaaacc tcctgtggtg
      181 tacgtttgca gggaaggaat tcacttcggc cggccggcct cgaagctcgc ggcacgccct
      241 gccaggttcg tgcccgtcgc cgccgacgtc attcgcggcg cttggccgcg cggcgccggc
      301 gccggtgatg gctcgcgcag gcgtctctgc aggcccgggc ccgcatcgtg tgatccgcgt
      361 cagtctcgct cggcggacgc gaggcgatgc gccgacagga tcgcctcgat cgccgacgac
      421 atggcgatct cgtcgtcgga ggcggtggcg atcgaacgct cgctgcccaa gatctcgcgc
      481 gagctcgcgt cgatcctcca gcgagccgtc cccgacaccg agccggtcac cgagaaattc
      541 acgcgacgat tgaccggcag cggcgactgt gtcaggctcg ccgtgagccc ggaacctacc
      601 taggagctca cggcgtggcg agccggaata ctcaacgcct ttcgtgacgg attatgactg
      661 ctgtccgcgg aatgacgatg accctgagaa ttacaattgg ttgagctgga catcgcggga
      721 agaagtcgcc cgctccctgc tcgaacggct gttcccgaga tttgcctcac agtcggggct
      781 caccgcaatg gagcgcggcc gggtgtggag cttggcgctc gccgtcgaac tcgcgccgct
      841 cgagcttgcg ccgctgctac tgcagatcgc aatcgatcct gatctgccag atcctgatct
      901 ggaggaaggc gagcggtgca cggtgctgtg caggctgcgg cggctgggcg tggcggtgcc
      961 tccgagcgcg ctgcgtcagc tgctcgagga tgcggttggg ccagcgccgc agatgtccgt
     1021 gatatgggag ctgctgcacc atacagactc gctcgacgag tccacgctcg cagtgctgga
     1081 tgcgctcccg ccggagcaac gcaattcgct cttccacgac tttgccgatg gcgacgacgt
     1141 gccgtgcaag gcggtccgcc atgcgctgtg gcgccgctgg gccggggacg acattgacct
     1201 cgcgatcgcc gccggaccga gttactccgg cgcgaccgag accgtcgcgg cctggctgaa
     1261 cgcggcgccg gcgagcgagg acgcaatggc cgccctcgcc cggtttccca gtctcgccat
     1321 cgagcacccc ggagccgcgg aggcactcgt gctgccgccg accctgctgc tggagcgact
     1381 cggcgtcgcg cggctgtaca cgctggtgca ccaggcggtc gtcctgcagg gctcggagcc
     1441 gcgatctccc acggcgaccg agttccagga cgctcgccgg tacgaggccg cgctctccgt
     1501 gctacgcaag atcccggagg ttcggggcga gctcgcacgg ttgctcgact ccgtcgtcct
     1561 cccgcctcgc gtgcacctgg atctggtccg gatcgcgctc gacatcgacc gcggcgcaac
     1621 actgacctac ctcgcccgat acccgaactt gtggcgcgac gtcgcgcgca cgctcgagga
     1681 gtcgccccgg cccggcgatc gtgcgttcgc ggaagcggcg gcacgctcga cggatccctt
     1741 cacggcgtat cacgcgatcg gcgtgctcga tcagctgggt gggccgctac cgccgctgcc
     1801 cgtcgcagtg ctgccgcggg ttcgcatgct gggagcgcac gcgcgacgcg gtgatcgagg
     1861 tgcggttgcc gcgttgatcg aagcaacgtc cgcacccgag gtcctggtgc gcgcggaggc
     1921 ggagcggtgg ctcggacggc tcgacgatgc acgggagcac ctcgccatct tcgagcgcgc
     1981 actggccgac cgcgaacggt gtatgtggcg ctgcccgccc cgccagcccg tggccgagca
     2041 ggctgcgatc gcattcgctc gtgcgggtgg ttctcgaacc ttgttgctgc gcacctactt
     2101 tgccgtgggc tcgaggtgcg ccgataccat cgaggatctc ctgtgtgacc ggccacccac
     2161 gctggagtca gagtcgttgc cgccgttcga tccgctcgat ctacccaggc cgtcatgggg
     2221 tcgcaagccg gcatacggaa agccttccgt agcgtattgt gtatgctgcc cgcatacgta
     2281 cgatgacaca gagaattaca actggttgag ctggacatcg cgtgaagaag tcgcccgctc
     2341 cctgctcgaa cagctgttcc cgactgccac gcagtccgag ctgaccgcga tcgcacccgc
     2401 gcacaagcca ctccagcggt ggcggacctt gagtctcgca accgcactcg cgccgctcac
     2461 gcttgcaccg ctgctcctgc ggatcgcgct cgatcctgat ctcaggcacg gggagctgtg
     2521 cgaggtgctg agccggctgc gggagctggg cgtggcggta cctccggata cgctgcgccc
     2581 gctgctccat cggctgctcg aaggcgcggt cgggcctgtc gggccacact ccgaggtcac
     2641 atggacggcc gaactcacag cgacgagcga actgctgcag tacgtggact cgctcgacga
     2701 gtccacgctc gccgtgctgg atgtgcaccc gccggcgcaa cgcaagctgc tgctccgccg
     2761 ctggaccgaa ggcgacgacg tgccgtgcaa ggcggtccgc gacgcactgt ggcgccgctg
     2821 ggccggcgac gacgttgacc tcgcgatcgc cgccggaccg agctaccccg gcacgacgga
     2881 gaccgtcgcg gcctggctga gcgcggcgcc ggagagcgag gacaccatgg acgccctcgc
     2941 ccggtttccg agcctcgccg tcgagcaccc cggggccgca gaggcgctcg cgctggcgcc
     3001 aaccctgctg cgagagcgac tcggcgtcgc gcagctgcgc gagctggtgc accaggcggt
     3061 cgtcctgcag agcgtgcagg agcgatctat accggaggcc gagttccagc gccctcgccg
     3121 gtaccaggcc gcgctctccg tgctgcgcga ggtcccggag gttcggggcg aggtcgcacg
     3181 cctgctcggc tccgtcgtcc tcaccccgcg cgtgcacatc gatctggtcc agatcgcgct
     3241 cggcatcgac cgcggtgcaa ccctgacgta cgtcgcccga tatccggacc tctggtgcga
     3301 ggtcgcgcgc acgctcgagg agtcgccgca gcccggcgat cgtgcgttcg ccgaggcggc
     3361 cgcgcgctcg acggatcccg gcacggggta tcacgcgatc ggcgtgctcg atcagctggg
     3421 cgggccgctg gagccgctac cgctcgcggc actgccacag gttcgcatgc tgggagcgcg
     3481 cgcgcagcgc ggtgaccgag acgcggtggc cgcgttgatc gaagcaacgt ccgcgcccga
     3541 ggtagcggtg cgcgcggagg cggtgcggtg gctcggacgg ctcgacgatg cacgggagca
     3601 cctcgccatc ttcgagcgcg cactgtccga ccgcgtacgg tgtggaaagt tctggccggg
     3661 ctacgcggtg gccgaccagg ccgcgatcgc actcgatcgc gcaggtggct ctcgaacctg
     3721 gttgctgcgc acctacttct cgctgcccta cgctggcggg tgcgcctccg acaccatcgt
     3781 gaagctcctg tgtgaccggc cacccatctc gcccagagat g
//
LOCUS       DASVOR010000229         9868 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148251,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000229 DASVOR010000000
VERSION     DASVOR010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148251"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VF516_05000"
     CDS             <1..225
                     /locus_tag="VF516_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107062.1"
                     /translation="RPEHAIAQYRAALPGLDEHDTHLLAHAARDRLGRLIGGDEGAAL
                     RAETRAWLDTEAVREPDTMLEMLLPGPITP"
     gene            complement(308..2068)
                     /locus_tag="VF516_05005"
     CDS             complement(308..2068)
                     /locus_tag="VF516_05005"
                     /inference="COORDINATES: protein motif:HMM:NF014869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M4 family metallopeptidase"
                     /protein_id="HEX8107063.1"
                     /translation="MVGGDFVLVMDGAGQILSKSTALEHPIDVPSITPTLSQGAAETI
                     ASQRLRSLTRVEGTQLVVHALGPTPRLAWESTVDGFGANGISRLTVHVDAITGAVLGE
                     QEHVLYGSGTSAWNGPNPVTLNTTQSGGTFSMRDPTITNLSCQDAANNTTFSGADDLW
                     GNGNATSRETGCVDALFGAQTEARMLSQWLGRNAMDGAGGAWPIRVGLAQVNAFYDGT
                     QVQVGHNNANQWIGSIDVIAHEMGHGVDDHTPGGISGSGTQEFVADTFGAATEWFANE
                     PAPFDVPDFTVGEQINLVGSGPIRNMANPSAVGDPNCFSSSIPTAEVHAAAGPGDHWF
                     YLVAEGSNPTNGQPTSPTCNGSTVVGLGPQTAIKIMYNAMLMKTTGSSYLRYRTWTLQ
                     AAINLFPGSCTQFNTVKAAWDAVSVPAQSTDPTCGGGGGGGGGGTNLLLNPGFESGAV
                     NWTATAGVINNTAGIARTGTFAGWLDGFGTTHTDTLFQTVAIPSTSTAAQLCYWLRIT
                     TAETTTTTQFDKLTVTIRNTANTVLSTLATFSNLNAGAYRQVCHNVGSFAGQTVRVFF
                     TGTEDSSLQTSFFIDDTSLQ"
     gene            2746..4275
                     /gene="argJ"
                     /locus_tag="VF516_05010"
     CDS             2746..4275
                     /gene="argJ"
                     /locus_tag="VF516_05010"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX8107064.1"
                     /translation="MRMTDRLVGRNTVKRVFRRHGLDPAPAVRARAGLRRCQDSSCGN
                     SAAAFRLISTRLGRCNLRSVDAAGARRLDRAPPAQLATGADRRDRGPLQAGRCEVQGH
                     DRARRRTRHGRSGRLPRVTKLVCSGFRFAGVAAGIKDDHRLDVGLIAADGPVSAAAVF
                     TRNQVAAAPVELSRTILARTRGRIRGVVVNSGNANACTGSNGTRDARQMAALAREACG
                     GHALVASTGVIGRPLAMDRLAGGIAAAGAALARDGFGRFAEAIMTTDKRPKVAARDVT
                     MGRRAVRLVGTTKGAGMIAPNMATTLTFVVTDAAVPVAALRSLTAAAVKPTFNAIAVD
                     GDTSTNDTLAVLAGGSGQPSSRELRTFGAALTDLLDDLAHQLIGDGEGVHHVVTIEVR
                     GARNTRDAEAVARRIAISPLVKTAIAGGDPNWGRILCAVGNAGAELRPDRIALAIGGV
                     PVVRAGAAIAGWDPAAVAAVMKTPAYAMTIDLGVGRAAARYLACDLSHDYVTINADYT
                     T"
     gene            complement(4288..9441)
                     /locus_tag="VF516_05015"
     CDS             complement(4288..9441)
                     /locus_tag="VF516_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8107065.1"
                     /translation="MLSPTYLTRPECAREWAGFEWRRASYGQPPELLLPVIWIPIPDG
                     QLPPAIQQRQYKHQALGPDYATHGLRHLVKRGGAPYRNFVEALAADVCAVMSQPAPPS
                     ASLPVPDQLKDPFAYLTDTEGLIDLLWDFNAAAMEPVSPAERAKGWHEPYADLSAYTV
                     AIDWPFPEPGRLDLVPSGKQGPSYSGNVNSFNWKRFYDDLGGGVFLESVKDRMRPEYD
                     YVLIDSRTGVSDSAGVCTVQMPDALVVCFTGNNQSIDGCASVVESVRAQWSSDGRWPD
                     RNRILPLLTRIDDSEKEKLDARRALVRATFQPYVAAIPASTDAYWRDTEVPYKPWYSY
                     EEVLAVFGDRYKENSSLLASAEALTRILTRGLVTEVVPPGDADREGVIAQFAATEKVR
                     EPVVAQGGVEFRPGSPRGAPAPLIDFTVERQRHARFVGREDVLARLDEWLLGPDEVGW
                     VVVTGGPGMGKSAILSAWLARREAAGAVVPHHFIRRQVASWDQPEVIAASLAAQIEAA
                     FPDLRDPAARPEGRLIELLGRVSKQLGSSERLVVVVDGLDEVAAEPGDNPLPRFLPHV
                     VPPGIRFLCAMRPTYPHLSWIEARASIQRLDLDDRPWAASSEAVVRGFWEAAAAEHEP
                     RLSAEMIDTAVERAEGNVLYAVMLHDALQGSPAPERRVDPIPRGIQGLIGEIWDRAAV
                     HEGVRAGLGLLCAAQEALPLDVIAELAEWGPDPYEERARFLREARQLLLEEPGSWSGG
                     VAYRLRHNWVRELIGERLGGATVRAHHGELWRELATWPAPVEPAERRYALRHGLIHRV
                     EASAWADAWRLAADTSFLEAKCRELGAAEAVADVARAAERCRVSGDEALRGRFVDLAR
                     ALGLESHWLRAAPEATTALVWNRLRQSGWSADDIDQQLQLPAGATFLRVHDVATRESS
                     ARMGDHVAHADRVNGCAVTPDGRRAVSASWDKTLKVWDLDSGQPLATLQGHASRVLAC
                     AVTPDGRRVVSVSWDSTLKVWDLDSGRLLATLEGHADGVNACAVTPDGRRVVSASSDR
                     TLKVWDLDSGQALATLQGHANRVRACAVTPDGRRVVSASEDRTLKVWDLDSGQALATL
                     QGHAERVLACVVTPDGRRVVSASWDHTLKVWDLASGECLLTHHGNSSFSAVAATTTAI
                     VAGDAGGAVWFLDWPWSSRSTRALHTVSAPEPPRRSHFNNEVFHEDFRPRSPIQKHTI
                     LFLPANPSEAGRLALDREARAIHNELNRSGSRESFSLETRWASEPLDLLRNLRKLKPT
                     VVHFSGHGGESVTGLFFQGSDGRGQRASARAIEEVFGAAGGSVRIVVLNGCYDDGLAE
                     ALLAHIDCVVGVSEAIRDDAARSFAIGFYGGLGDGASVAEAYKYGIAAIHLGGLHDSA
                     LPQLQVRAGVDASQLVLAADLQTIGGIESFAAASPGMRSIESGASASSSVDIGIITIR
                     DDEFRALLGVFSSRAGTFQGASREYTLRHADAGNGEQYTVAILRQVEQGNAEAQDAAR
                     DLIEDLAPRLVLVVGIAGGAPSDDLKLGDVILSTRIEGFTVDARGFEQSLMYAATSPV
                     DKALAASIANLAAREDEMGDWTADLPPQPPVPSTRKGQLYGPPEWQRSLRATLEHHYG
                     KGATPRAPVYAVGPIASSDRLVKDPKQLIPWLTTARNVLAVEMESGGVYRAAQERCPM
                     LAIRGISEIVGLKRADAWTKYACAVAAAFTRAFLRTRPIPARRSMPGPG"
     gene            complement(9632..>9868)
                     /locus_tag="VF516_05020"
     CDS             complement(9632..>9868)
                     /locus_tag="VF516_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107066.1"
                     /translation="ACAAAARSAGGARSPRRSPAVMAVLAAAYLIIARAHLLDLVAET
                     WDHGAAPRQADHGRRDSMRSCATWRRHPRCPTSS"
BASE COUNT         1521 a         3442 c         3381 g         1524 t
ORIGIN      
        1 cgccccgagc acgcgatcgc gcagtaccgc gccgcgctgc ccgggctcga cgagcacgac
       61 acccacctgc tcgcccacgc cgcgcgcgac cggctcggcc ggctgatcgg cggcgacgag
      121 ggcgccgcgc tgcgcgccga gacgcgggcc tggctcgaca ccgaggcggt gcgcgagccg
      181 gacaccatgc tcgagatgct gctgcccgga ccgatcaccc cgtgatcgcc agggcccgcc
      241 ggaggcccgt accccgcttg tctgtcctac ccgcttcctt gtcctaccgg cgaggctggc
      301 cgcgagctca ctgcaagctg gtgtcgtcga tgaagaagct cgtctggagc gagctgtcct
      361 cggtgccggt gaagaacacg cgcaccgtct ggcccgcgaa gctgccgacg ttgtggcaga
      421 cctgcctgta cgcgccggcg ttcaggttgc tgaacgtggc gagcgtgctg agcaccgtgt
      481 tcgccgtgtt gcggatcgtg acggtcagct tgtcgaactg cgtggtcgtg gtggtctcgg
      541 ccgtggtgat gcgcagccag tagcagagct gcgccgccgt cgaggtcgac gggatcgcca
      601 ccgtctggaa cagcgtgtcg gtgtgggtgg tgccgaagcc atccagccag cccgcgaagg
      661 tgccggtgcg ggcgatgccg gccgtgttgt tgatgacgcc ggcggtcgcc gtccagttca
      721 cggcgccgct ctcgaagccc gggttgagca acaggttcgt cccgccgcca ccgccgccac
      781 cgccgccgcc gcaggtcggg tcggtggact gagcagggac gctgaccgcg tcccaggcgg
      841 ccttgacggt gttgaactgc gtgcagctgc cgggaaacag gttgatcgcg gcctgcagcg
      901 tccaggtccg gtatctcaag tacgagcttc ccgtggtctt catcagcatc gcgttgtaca
      961 tgatcttgat cgcggtctgc gggcccaggc cgacgaccgt ggaaccgttg caggtcgggc
     1021 tggtcggctg cccgttggtc ggattcgagc cctcggcgac caggtagaac cagtggtcgc
     1081 cgggaccggc cgcggcatgg acctcggccg tgggaatcga ggacgagaag cagttcgggt
     1141 cgccgaccgc ggatggattg gccatgttcc ggatcgggcc gctgccgacc aggttgatct
     1201 gctcgcccac ggtgaagtcg ggaacgtcga acggcgcggg ctcgttggcg aaccactcgg
     1261 tcgccgcgcc gaacgtgtcg gcgacgaact cctgcgtgcc gctgcccgag atccctccgg
     1321 gggtgtgatc gtcgacgccg tgacccatct cgtgggcgat cacgtcgatc gagccgatcc
     1381 actggttggc gttgttgtgt ccgacctgga cctgggtgcc gtcgtagaat gcgttcacct
     1441 gggcgaggcc gacccggatc ggccaggcgc cgcccgcgcc atccatcgcg ttgcgaccga
     1501 gccactgact cagcatccgg gcctcggtct gcgcgccgaa cagcgcgtcc acacagccgg
     1561 tctcccggct ggtggcgttg ccattccccc acagatcgtc cgcgccgctg aaggtcgtgt
     1621 tgttggctgc gtcctgacag ctcaggttgg tgatggtcgg gtcgcgcatc gagaacgtgc
     1681 ctcccgactg cgtcgtgttg agcgtgaccg ggttgggccc gttccatgcc gaggtcccgc
     1741 tgccgtacag gacgtgctcc tgctcgccca gcacggcgcc ggtgatcgca tcgacgtgca
     1801 cggtcagccg gctgatgcca ttggcgccga agccgtccac ggtggactcc cacgcaagcc
     1861 gcggggtggg gccgagggcg tgaaccacga gctgggtacc ctccacgcgg gtcaggctac
     1921 gcagccgctg gctcgcgatc gtctctgcgg cgccctggct cagcgtgggc gtgatcgaag
     1981 gaacgtcgat cgggtgctcc agcgcggtcg acttggagag gatctggccc gcgccgtcca
     2041 tcaccagcac gaagtcgcca cccaccacgg gaagcccagc gtgcgtgcgc tcgtacgctg
     2101 cataaaagag tccactggac gactccatgc cgcgctgcac gaatgcgtcg ttggggctga
     2161 cgtggagaaa cgccggccgg ctggccacga gcgcggctgc actgccgctg gcgacggcgt
     2221 aggccgcgct aacgccgggt gtcgtcgggg ctccgatgac ctttgctggc gtgtcatccg
     2281 gcgtcgctgc tgcgcccggg gcgtccagat caagggcgca gcctgcgaag agagctgccg
     2341 tcgttaacgc cgtaagaagg gatccaatct ttctgttcat caactgcctc caggacggga
     2401 cgagacgaga cgagacgcac ggcgatcgca tcggcagacc gttgccgagc acccgccgcg
     2461 attccgcatg tgaactatca aaaacgattt gaaccgtcta ttcaaatgga gtgcttctct
     2521 caaaattctg ccggtaccca tctgccagga ggtgcgctga tgtgtgtctg gccaaggtgg
     2581 gatcgcaacg acggcagcac ctcgaactct cgcaactcga gtagccacct gtccagtggg
     2641 cggccacgcc gacgtgcaac aacgcggcaa gtcatctcga cgcgtggaac accagagttc
     2701 catccgtgct cgacccatcg atctggtttc gcggagttga cgcgggtgcg catgacagac
     2761 cgactagtcg gccgcaacac ggtgaagcgc gtctttcggc ggcacggcct tgacccggca
     2821 cctgcggtgc gtgctcgtgc tggcttgcgc cgctgccagg acagctcctg cgggaactcc
     2881 gctgcggcgt tccggctgat cagcacgcgg ctcggcaggt gcaaccttcg ttcggtggat
     2941 gcagcgggcg cccgacggct cgatcgagct ccacctgcgc agcttgccac cggcgccgat
     3001 cgccgagacc gtggtccgct acaagccggg cgatgcgaag tacaaggaca tgatcgcgca
     3061 cgtcggcgga ctcgccacgg gcgatcgggt aggctgccgc gcgtgaccaa gctggtgtgc
     3121 tccgggtttc ggttcgcagg cgtcgccgcc ggaatcaagg atgaccatcg tctcgacgtt
     3181 ggcctgatcg cggccgatgg gccggtgagc gcagcggcgg tgttcacccg caatcaggtc
     3241 gccgcagcgc ctgtcgagct atcgcgtacg atcctcgcgc gcacccgggg ccggatccgc
     3301 ggcgtggtgg tcaacagcgg caacgccaac gcgtgcaccg gctcgaacgg cacgcgggat
     3361 gctcgccaga tggcggcgct ggcgcgcgag gcatgcggcg ggcatgcgct cgtcgcatcg
     3421 accggtgtga tcggccggcc cctggcgatg gaccgactgg cgggcgggat cgcggccgca
     3481 ggtgcggcac tcgctcgcga cgggttcggc cgcttcgccg aggcgatcat gaccaccgac
     3541 aagcggccca aggtcgctgc tcgagacgtc acgatggggc gccgcgcggt gcgactggtc
     3601 ggcacgacca agggcgcggg catgatcgcg cccaacatgg ccacgacgct gacgttcgtg
     3661 gtcaccgatg ccgcggtacc ggtcgccgcg ctgcgctcgc tgaccgccgc tgccgtgaag
     3721 cccacgttca acgcgatcgc ggtcgatggc gacacctcga ccaacgatac gctggcggtg
     3781 ctggccggcg gtagcggaca gccgtcgtcg cgcgagctgc gcacgttcgg cgcagcgctg
     3841 accgatctgc tcgacgacct ggcgcaccag ctgatcggcg acggagaggg tgtccatcat
     3901 gtggtcacga tcgaggtgcg cggcgcacgc aacacgcgcg acgcggaagc agtggctcga
     3961 cggatcgcga tctcgccgct ggtcaagacc gcgatcgccg gcggcgatcc caactgggga
     4021 cggatcttgt gcgcggtggg caacgccggc gcggagctcc ggcccgaccg gatcgccctc
     4081 gccattggcg gcgtgccggt cgttcgcgct ggcgctgcca tcgcgggctg ggacccggcg
     4141 gcggtcgcgg ccgtgatgaa gacccccgcg tacgcgatga cgatcgatct cggagtcggc
     4201 cgcgcggctg cgcgctacct ggcatgcgat ctcagccacg actacgtgac gatcaacgcg
     4261 gattacacga cctgatacgg tgggacgtca accaggtccg ggcatacttc gtcgagcagg
     4321 aatgggtcgc gtgcgcagga aggcgcgggt gaaggcggcg gccactgcac aggcatactt
     4381 cgtccaggcg tctgcgcgct tcagcccgac gatctcgctg atgccccgga tcgccagcat
     4441 cggacatcgc tcctgggcgg cgcggtagac tccgccggat tccatctcga cggcgaggac
     4501 gttgcgagcg gtcgtcagcc aagggatcag ctgcttggga tccttcacca gccggtcgct
     4561 cgacgcgatc ggccccactg cgtagacggg cgcgcgcggc gtggcccctt taccgtagtg
     4621 gtgctccagc gtagcccgca gcgatcgctg ccactccggt ggcccgtaga gctgtccctt
     4681 gcgcgtcgat ggcacgggcg gttgtggcgg tagatcggcg gtccagtccc ccatctcgtc
     4741 ttctcgcgct gcgaggtttg cgatggaagc cgccagggcc ttgtcgacgg ggctcgtggc
     4801 tgcatacatg aggctctgtt cgaaccctcg ggcatcgacc gtgaagccct cgatcctcgt
     4861 cgagaggatc acatcgccga gcttcaggtc gtccgaagga gctccgccgg cgatccccac
     4921 caccagcacg agcctgggag cgagatcctc gatgaggtcc ctggcggcgt cctgggcctc
     4981 ggcattgccc tgttcgacct ggcgcaagat ggcgaccgtg tattgctcac cgttgcctgc
     5041 gtccgcgtgg cgcagcgtgt actcgcggct ggccccctgg aaggtgccgg ccctgctcga
     5101 aaacacgccc agcaaggcgc ggaactcgtc gtcacggatc gtgatgatgc cgatgtccac
     5161 gctcgaggag gcagacgcac cactctcgat gctccgcatc ccaggagagg ctgcggcgaa
     5221 gctctcgatg cccccgatcg tctgaaggtc cgcggccagg acgagctggc tggcgtcgac
     5281 gccggcgcgg acctggagct gcgggagagc gctgtcgtgc aggcccccca ggtggatcgc
     5341 agcaattcca tacttgtacg cctccgcaac cgacgcgccg tcgcccaggc caccatagaa
     5401 gccgatcgcg aagctccggg ccgcatcatc gcggatcgcc tcactcaccc ccacgacaca
     5461 gtcgatgtgt gccagcaacg cttcggccag gccatcgtca tagcagccat tgaggaccac
     5521 gatccggacg gagcccccgg ccgcgccgaa cacctcctcg atcgcccggg ccgatgcacg
     5581 ttgaccgcgg ccgtcggagc cctggaagaa caggcccgtc acactctcgc cgccgtgacc
     5641 gctgaagtgg accactgtcg gcttcagctt gcgcaagttg cggagtagat cgagcggctc
     5701 cgaggcccat cgtgtctcga ggctgaagga ctcccgggat ccgctgcgat tcagctcgtt
     5761 gtggatcgcg cgtgcctctc gatcgagcgc gagcctaccg gcttcactcg ggttggctgg
     5821 cagaaacagg atcgtgtgct tctggatcgg cgacctcggc cgaaagtcct cgtgaaagac
     5881 ctcgttgttg aaatgagatc ggcgtggggg ttcaggagca gagacggtgt ggagagcgcg
     5941 cgtgctgcga ctcgaccacg gccagtccag aaaccagacg gcgccgccgg cgtcgccggc
     6001 gacgatcgcg gtcgtggttg ctgcgacagc ggaaaaggag gagttgccat ggtgggtgag
     6061 aaggcactcg cccgacgcca ggtcccagac cttgagcgta tggtcccacg acgcggagac
     6121 cacgcgccga ccgtccggcg tcaccacaca cgcgagcacc ctttcggcgt ggccttgcag
     6181 ggtggccagc gcttgcccgg agtcgaggtc ccacaccttg agcgttcggt cttccgaggc
     6241 cgagaccacg cgccggccgt ctggcgtcac cgcgcacgcg cgcaccctgt tggcgtggcc
     6301 ctgcagggtg gccagcgctt gcccggagtc gagatcccac accttgagcg tcctgtcaga
     6361 agaggccgag accacgcgcc ggccgtccgg cgtcaccgcg cacgcgttca caccgtcggc
     6421 atggccttcc aacgtggcca ggagtcgccc cgagtcgagg tcccacacct tcagcgtgct
     6481 gtcccaggag accgagacca cgcgccggcc atccggcgtc accgcgcacg cgagcacccg
     6541 ggaggcgtga ccttgcaggg tggccagggg ttgccccgag tcgaggtccc acaccttgag
     6601 cgtcttgtcc caggaggccg agaccgcgcg ccggccatcc ggcgtcaccg cgcatccgtt
     6661 caccctgtcg gcgtgggcta cgtgatcgcc catgcgcgcc gagctctccc gggtcgccac
     6721 gtcatgcacg cggaggaagg tggctcctgc cggaagctgc aactgctgat cgatgtcgtc
     6781 tgcgctccac cccgactgcc gcagccggtt ccacaccagc gccgtggtcg cttccggtgc
     6841 agctcgcagc cagtgcgatt cgagcccgag cgcccgtgcc agatcaacga accgaccgcg
     6901 gagcgcctcg tcaccgctga cccggcaacg ctcggccgca cgcgccacgt cggccacggc
     6961 ctcggccgcg cccagctcgc ggcacttcgc ctccaggaag ctcgtatcag cggccaggcg
     7021 ccacgcgtct gcccacgcgc ttgcctcgac gcgatggatc agcccgtggc gcagcgcgta
     7081 ccgccgctcc gccggttcga ccggggccgg ccaggtcgcc agctctcgcc agagctcgcc
     7141 gtgatgggcg cggacggtcg cgccgccgag ccgctccccg atcagctcac gcacccagtt
     7201 gtggcgcagg cgatacgcca caccgccgga ccacgacccg ggctcctcga gcagcagctg
     7261 ccgcgcctcg cgcaggaagc gggcccgctc ctcgtagggg tcggggcccc actcggcgag
     7321 ctcggcgatc acgtcgagcg ggagcgcctc ctgcgccgcg cacaggagcc ccagccccgc
     7381 acgtacgccc tcgtgcaccg ccgcccgatc ccagatctcg ccgatcagcc cctggatccc
     7441 acgtgggatc ggatccacgc ggcgctccgg cgcaggcgag ccttgcagcg cgtcgtggag
     7501 catcacggcg tacagcacgt tgccctcggc acgctcgacg gcggtgtcga tcatctcggc
     7561 cgagagtcgc ggctcgtgct cggcggcggc ggcctcccag aacccgcgca ccaccgcctc
     7621 gctggacgcc gcccacgggc gatcgtcgag atcgaggcgc tggatcgacg ctcgagcctc
     7681 gatccagctc aggtgcggat aggtcggacg catcgcgcac aggaaccgga tccccggcgg
     7741 caccacgtgc ggcaggaagc gcggcagggg gttgtcgccg ggctccgccg cgacctcgtc
     7801 gaggccgtcg accaccacca ccaggcgctc ggacgagccc agttgcttcg agacccggcc
     7861 cagcagctcg atcagccggc cctcgggcct cgccgcggga tcccggagat cgggaaacgc
     7921 cgcctcgatc tgggcggcca gcgacgcggc gatcacctcg ggctggtccc agctggcgac
     7981 ctggcggcgg atgaagtgat gcggcaccac ggcgccggcg gcctcgcgcc gcgccagcca
     8041 cgccgacagg atcgcgctct tgcccatccc cggacccccg gtgaccacga cccagccgac
     8101 ctcgtcgggt ccgagcagcc actcgtcgag gcgcgccagg acgtcctcgc gcccgacgaa
     8161 ccgggcgtgt cgttgccgct ccaccgtgaa gtcgatcagc ggcgcgggcg caccccgcgg
     8221 ggaccctgga cggaactcga caccaccttg cgcgacgacc ggttcgcgca ccttctcggt
     8281 agccgcgaac tgcgcgatca ccccctcgcg atcagcgtcg ccgggaggca cgacctccgt
     8341 gaccaagccg cgggtgagga tccgggtcag cgcctcggcc gaagcgagga gggagctgtt
     8401 ctccttgtac ctgtccccga agacggcgag cacctcctcg tacgagtacc agggcttgta
     8461 cggcacctcg gtatcccgcc agtaggcgtc ggtcgacgcg gggatcgctg cgacgtaggg
     8521 ctggaacgtc gcgcgcacga gcgcccggcg tgcatcgagc ttctccttct cggagtcgtc
     8581 gatccgggtc aagagcggca ggatgcggtt gcgatcgggc cagcgtccat cgctgctcca
     8641 ctgtgcacgc accgactcca cgaccgaggc acagccatcg atgctctggt tgttgcccgt
     8701 gaagcaaacg acgagcgcat cgggcatctg caccgtgcag acccccgcgc tgtcgctcac
     8761 cccggtgcgg ctatcgatga gcacgtagtc gtactccggc cgcatccggt ccttcaccga
     8821 ttccaggaac acgccgccgc cgagatcgtc gtagaagcgc ttccagttga acgagttcac
     8881 gttgcccgag tagctcgggc cctgctttcc ggacggcacc aggtcgaggc gtcccggctc
     8941 cggaaacggc cagtcgatcg cgacggtgta cgccgagagg tcggcgtacg gctcgtgcca
     9001 gcccttcgcg cgctcggccg gggagaccgg ctccatcgcg gcggcgttga agtcccacag
     9061 caggtcgatc agcccctcgg tgtcggtcag gtacgcaaat ggatccttca gctgatcggg
     9121 taccggcagc gaggccgatg gcggcgcggg ctggctcatc accgcacaca cgtccgcggc
     9181 gagcgcctcc acgaagttgc gatacggggc gcccccgcgt ttcaccaggt gacgcaggcc
     9241 gtgggtcgcg tagtcgggcc cgagcgcttg atgcttgtac tgccgttgct ggatcgccgg
     9301 tggcagctgg ccatccggga tcgggatcca gatgaccggc aacagcagct cgggaggctg
     9361 gccgtacgac gcgcgacgcc actcgaagcc ggcccactcc ctggcgcact ccggccgggt
     9421 gaggtaggtc ggcgacagca tcgcaggaag gtccggctgt cgagcaacgc tcgttcgatc
     9481 tgcggcttcc acagcgcgcc gagcgagatg gcctcggtgt cacggaaggc cagaccgtcg
     9541 agggtggtcc ggccgagctt gccgcgcagc tcgtcccgga agtccttgta gaagcgctcg
     9601 aagaacttgt ccacatctgc ccgggcatag ctcaggaaga agtagggcac ctcgggtggc
     9661 gtcgccacgt cgcgcagctt cgcatggaat cacggcgacc gtggtcggcc tggcgcggcg
     9721 ccgcgccatg gtcccaggtc tcggcgacca gatcgagcag gtgcgcgcga gcgatgatca
     9781 ggtacgccgc cgccagcacc gccatcacgg cgggcgagcg cctgggcgag cgagcgccac
     9841 cagccgagcg ggccgcggcc gcgcacgc
//
LOCUS       DASVOR010000230         8252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265079,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000230 DASVOR010000000
VERSION     DASVOR010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8252
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265079"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF516_05025"
     CDS             <1..459
                     /locus_tag="VF516_05025"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8107067.1"
                     /translation="WLRCAPRVAPVVDPQAWVTVVLRRLVIDRLRRRRAAAEVPAEGV
                     VLAVVEPEPVPWWCDLELGVVRRELGRLPPALRETFELFSFQARSYQQIACELNIAMG
                     TVGTRISRARALLRRRLSDHWAAGCGAAADSARMVGGGSRSQAAGVLASR"
     gene            416..1060
                     /locus_tag="VF516_05030"
     CDS             416..1060
                     /locus_tag="VF516_05030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8107068.1"
                     /translation="MDPDLRLLESWRAGDGRAGQDLFNRHFADIYRFFEYKVGADADD
                     LAQRTFMACVDARDRFRGGSSFRTYLFAIARNQLYSFLRRLPRAEHVDFEHTSIADLV
                     PSLTSQLGRARDIERLRLALTTLPAEQQLLLELHYWHELDAEALGEVFEAAPGTIRVR
                     LLRARRALRQRMEQIDADRQRAGAASEPGSGEASEDSLLSALSQPEPAELGGEP"
     gene            1104..1979
                     /locus_tag="VF516_05035"
     CDS             1104..1979
                     /locus_tag="VF516_05035"
                     /inference="COORDINATES: protein motif:HMM:NF044908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8107069.1"
                     /translation="MYADLAADTAVRRDPDTPGRYHIALPDHWDFLMPSGGVVMTCAL
                     RAAAAELADPSLRFGSATTIFCTPIRNGPLVIDVVVLRRGGGAAQVRAALRDGSGEPG
                     IEVLATFLRERKGPDVKGVAFPEVRSLDEALPVDNSSRSSPHVLLRFHRQLEYRIAEG
                     EPFWQPSDVAGPARYARWVRYRTPQRDAEGRLDRLALPPLIDMMPGALHRAIGPGSYR
                     FFAPSLDLTTYVIDDTRREWLLFAVTARRARAGWAIADTEVWDDEGRYIGHGSQGMFI
                     RGAAGEPPVVDASNR"
     gene            complement(2041..2229)
                     /locus_tag="VF516_05040"
     CDS             complement(2041..2229)
                     /locus_tag="VF516_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107070.1"
                     /translation="MDPFSSGISFPDWKELQDQAWMWPVRETYDPLIVRRPTTWLLAK
                     GWKLAGEISAREVPSRRR"
     gene            2336..2530
                     /locus_tag="VF516_05045"
     CDS             2336..2530
                     /locus_tag="VF516_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107071.1"
                     /translation="MIAIREHLATTAPRSIPERGVDVPRRRDRETLHPACERSLVIGL
                     DDQVDVRALQGDMHDPEPLA"
     gene            3340..4305
                     /locus_tag="VF516_05050"
     CDS             3340..4305
                     /locus_tag="VF516_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8107072.1"
                     /translation="MRTSQLTLPLIAMWLAACHAGAPAAPVAEPAEGQVPGDGAALFY
                     RLVGSGPDVLVMIHGGPGMDMGYMVPDFAPLAERHRLLFYDQRGGGRSERLRDDPALF
                     TMAHHVADLEALRVRFGLARMTLVAHSFGPAIAAAYAIAHPDHVARMIFLGPVPPRQG
                     EFEKRYGATLDGRLTDAERAEMARLEDAMVHGADPVAACRAYWAIAVKPRVARPELVS
                     RVTGDFCSAGAEAIRAGYSIAGPHTTESLGAWDFRPDLARVAAPTLIVHGEDDAIPMD
                     LVAEWTSALPHARLIKVPGASHFPYVERPDLVWPAIEDFLRATPP"
     gene            complement(4308..4997)
                     /locus_tag="VF516_05055"
     CDS             complement(4308..4997)
                     /locus_tag="VF516_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107073.1"
                     /translation="MAACAPRAVTPPTRTFVMDSPSAPAVGHSDVQLDAASIIGEMLG
                     PRLVGGDARVRHTVEPGVALEADGGVLHVTNNGQGGDRNGYTGRLGVLLHSQDRHWGL
                     GAGVGGGLSPTAGTWGAADVHGVVAGTNHYVRPMLGAGLGYSGPLGHHPFTVTLPGES
                     PPGTTLQLPHNAIAQLHAGLELGPPDTAVILGLSLSRFWLREDSRVDNPSSHRQDNFA
                     ALGIGLRIAID"
     gene            complement(5173..6162)
                     /locus_tag="VF516_05060"
     CDS             complement(5173..6162)
                     /locus_tag="VF516_05060"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HEX8107074.1"
                     /translation="MSTQAPEPTGLAHAYLLDGRGGCEHLDWNGVVRWTPADGPLWLN
                     LDYTVADVQHWLAERSAIDPVVRDALLDDDPRPRAAAHGDALLLIVRAINLNEGAAPE
                     DMLSIRAWIEPHRMVTLRHRRSRSIKAIAGELDRGRGPAGVADLTASLVERVLEYAAT
                     RVDTLSDAIAACEDQVLTETRGELRAQLADHRRRAIALRRFLAPQREAFAKLAQIQLP
                     WLDATARARIVESADRMTRTVEELDAARDRAAVTQEELQSKIAEATNQRLYVLAIITA
                     VFLPLGFVCSLLGVNVGGVPLQHEDWAFWALCGVFVVTVALQIWFFRRRGWFG"
     gene            6280..7692
                     /locus_tag="VF516_05065"
     CDS             6280..7692
                     /locus_tag="VF516_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8107075.1"
                     /translation="MSPIDSTIPYYPVNDFGPLMKGLVIGGVGILHVFLAQFAIGGGM
                     LMWYFERLGQRGLEDARTFVDGYFKVLVLVSFVVGALTGVAMWFTTIQVGARTIGLMI
                     DEFHWLWATEWVWFAVEITAGYAFYRCGPRLAGRARLRLLELYALASWMSLFWINGIL
                     AWQLTPGRWLDGGGVWAGFFNPSFWPSLLFRTAVSTTLGALVGCIVINTMDLPRERRA
                     ALIRRAARFAAPIAAMPVLALWFLAVLPGDSRSWLLGGSMAMTMFVGIAAAASFLIGG
                     YAIVGLIVQRLYINGATATLLLGLAFGATAAGEFVREGARKPYTVRGVLYSTSIRPAE
                     VAALRAAGATAGDPYPLRDHYPTEQLARGARVERALCDACHTMRGANALVELTRTWTD
                     DQLRLNLAKLQRTKGFMPPFAGNADDVEALVQLLRWERDGRPKVAAAPPTSPETLAQI
                     ARWLDEAGTASAGEASGEVP"
     gene            7692..>8252
                     /locus_tag="VF516_05070"
     CDS             7692..>8252
                     /locus_tag="VF516_05070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107076.1"
                     /translation="MWPFDGSIGSPVESLWLALYLVTFALHAVLVSYVAAGAGYALVA
                     AARRSADPIAARTRDWLPFMLGAGITAGVGPLLFLQLLYQRRFYTANLLMGPRWGAVV
                     PALIVGFYALYLAKASERRRRLGLGVATACFAFVAWSWTELHQVMLDDAGWRAMYAAG
                     ERFHGGAAILPRLATWLGAMLALFATV"
BASE COUNT         1056 a         2955 c         2992 g         1249 t
ORIGIN      
        1 tggctgcgct gcgcgccgcg cgtggctccg gtggtcgacc cgcaggcgtg ggtcaccgtc
       61 gtgctgcggc gcctggtgat cgaccggctg cgccggcggc gggcggccgc cgaggtgccc
      121 gccgagggcg tggtgctggc cgtcgtcgag cccgagccgg tgccgtggtg gtgcgacctc
      181 gagctcggcg tggtgcgccg cgagctcggc cggctgccgc ccgcgctgcg cgagacgttc
      241 gagctgttca gcttccaggc ccggagctac cagcagatcg cctgcgagct gaacatcgcg
      301 atggggaccg tcgggacgcg gatcagccgc gcccgtgcgc tgctgcggcg ccggctgagc
      361 gatcactggg cggcgggctg cggggcggcg gccgacagtg ctaggatggt cggcggtgga
      421 tcccgatctc aggctgctgg agtcctggcg agccggtgac ggtcgcgccg gccaggatct
      481 gttcaaccgc cacttcgcgg acatctaccg gttcttcgag tacaaggttg gcgcggacgc
      541 cgacgacctg gcacagcgga cgttcatggc ctgcgtcgac gcgcgcgacc ggttccgcgg
      601 cggatcgagc ttccggacct atctgttcgc gatcgcgcgc aaccagctgt actcgttcct
      661 gcgccggctg ccccgcgccg agcacgtcga cttcgagcac acgtcgatcg cggatctggt
      721 cccgtcgctg accagccagc tcggccgggc gcgcgacatc gagcgactgc ggctcgcgct
      781 gaccacgctg cccgccgagc agcagctgct gctcgagctg cactactggc acgagctcga
      841 cgccgaggcg ctcggcgagg tcttcgaggc tgcgcccggg acgatccggg tccggctgct
      901 gcgcgcccgg cgcgcgctgc gccagcggat ggagcagatc gacgccgacc ggcagcgcgc
      961 gggggcggcg agcgagcccg ggtccggtga ggccagcgag gacagcctgc tcagcgcgct
     1021 gagccagccc gagcccgccg agctcggcgg cgaaccgtag atcgggccgc ggcgacgcgg
     1081 cgcattgcgt taaggtagcc gcgatgtacg ccgacctcgc cgccgatact gcggtgcgcc
     1141 gcgatccgga cacccccggg cgctaccaca ttgcgttgcc ggaccactgg gatttcctga
     1201 tgccgtcggg cggcgtcgtg atgacctgcg cgctgcgcgc ggcggccgcc gagctcgccg
     1261 atccgagcct gcggttcggg tcggcgacga cgatcttctg cacgccgatc cggaacggcc
     1321 cgctggtgat cgacgtcgtg gtgctgcggc ggggcggcgg cgcggcccag gtccgcgcgg
     1381 ccctgcgcga cggctcgggc gagcccggga tcgaggtcct cgcgacgttc ctgcgcgagc
     1441 gcaaggggcc cgacgtcaag ggcgtcgcgt tccccgaggt gcgctcgctc gacgaggcgc
     1501 tgccggtcga caacagctcg cggtccagcc cgcacgtgct gctgcggttc caccgccagc
     1561 tcgagtaccg gatcgccgag ggtgagccgt tctggcagcc gagcgacgtc gccggcccgg
     1621 cgcgctacgc ccgctgggtg cgctaccgca ccccgcagcg cgacgccgag ggccggctcg
     1681 atcggctcgc gctgccgccg ctgatcgaca tgatgccggg cgcgctgcac cgcgcgatcg
     1741 gcccgggcag ctaccggttc ttcgcgccga gcctcgatct cacgacctac gtcatcgacg
     1801 acacccggcg cgagtggctg ctgttcgcgg tgaccgcgcg gcgcgcccgc gccggctggg
     1861 cgatcgccga caccgaggtg tgggacgacg agggccgcta catcggccat ggctcgcaag
     1921 gcatgttcat ccgcggcgcc gctggcgagc cgccggtggt cgacgcctcg aaccgctgag
     1981 cggcgggctc ctttcacgtg catccctggc gaccgccatc ccgatcgaat gcgcctcggg
     2041 tcaccgccgc cggctcggca cctcgcgggc gctgatctcc ccggcgagct tccagccctt
     2101 cgccaggagc cacgtggtcg gtctccggac gatcagcgga tcgtaggtct cgcggaccgg
     2161 ccacatccag gcctggtcct gcagttcctt ccagtcgggg aaggagatac cggaggagaa
     2221 cgggtccacg agccacgtgc tcggcaatcc ctcgcgatct tctccatgct ttcgccagtt
     2281 cgagaggcag tacgagatcg catggcgcgc gggtcggcgt ggtgatgacc tccacatgat
     2341 agcgatccgt gaacaccttg ccacgacggc gccgcgttcc atccccgagc gcggtgttga
     2401 tgttcctcgc cgccgagatc gtgaaaccct gcatcccgcg tgcgagcgat cgcttgtgat
     2461 cggcctcgac gatcaggtgg acgtgcgtgc gctgcaggga gacatgcacg atccggaacc
     2521 gctcgcgtag cgcgacggtg atcgtggcct cgcggatcgc cttgtacatc gcgcgccggc
     2581 gcaggctgcc gacctccggc gcgacccgca tcacgacgtg cagcgggtga tacggcttga
     2641 actccggccg cgccgcatgg cgctcaccgg cgcgcgtacc cctgggcttg cgccctgcac
     2701 cgcgccgctt gccaccccgc cggaacagct cctgctgacc tttccgcttc ggctggcgct
     2761 tcgacctcgg cgacctcatg cgcgcatcgt taccacgcat gcaccacctg tcaatgattg
     2821 agcagtactg attgttattt attttcgtca cttatcggtc gttgaatgat gatcaagaaa
     2881 gcatctccat aacgacactt gtcctgaaaa ggacacattc catcatgact cgtacacgac
     2941 atagtgatcc cggaaacgaa tcaagaattc tctggaaggg agcgcccgcg cggggcgatg
     3001 cactgtgagt gggccgccct ggtcagcctg gtgcgcttca acgggccagt cacagggcgg
     3061 tccacgcggt ctcccgtgtt cctggccgca gccggcggcg ccgatgatgg tggtccctcg
     3121 ttgactgttc gacgcggagc aaccgtgcgc ctggtgcgag atcgtcgtcg caaccctggg
     3181 ggtggcgcgg cgagtgctcc gggcgcaggt gctgcagcag ctgcaccgtg atcgcggccg
     3241 ggaacttcgg ggggcgcgcg gacggagtgc tgcgaggatc gccacctggc ggatcgcgca
     3301 gcgcaggcag cccggcgcca gctgtgcgat ggtccgccgg tgcgaacctc tcagctgacg
     3361 ctacctttga tcgcgatgtg gctcgccgcg tgtcatgccg gcgcgccggc ggctccggtc
     3421 gccgagcccg ccgagggcca ggtccccggc gacggcgcgg cgctgttcta tcgcctggtc
     3481 ggcagcggcc ccgacgtgct ggtgatgatc cacggtgggc cggggatgga catgggctac
     3541 atggtgccgg atttcgcccc gctggccgag cggcaccgcc tgctgttcta cgatcagcgc
     3601 gggggcggcc ggtccgagcg gctgcgcgac gatccggcgc tgttcaccat ggcgcaccac
     3661 gtggccgacc tcgaggcgct gcgggtccgg ttcggcctcg cgcggatgac gctggtcgcg
     3721 cactcgttcg ggccggcgat cgcggcggcc tacgcgatcg cgcaccccga ccacgtcgcg
     3781 cggatgatct tcctcgggcc ggtgccgccg cgccagggcg agttcgagaa gcgctacggc
     3841 gcgacgctcg acggccggct gaccgacgcc gagcgcgccg agatggccag gctcgaggac
     3901 gcgatggtcc acggcgccga cccggtggcg gcgtgccgcg cgtactgggc gatcgcggtc
     3961 aagccgcggg tcgcgcggcc ggagctggtg agccgggtca ccggcgactt ctgcagcgcg
     4021 ggcgccgagg cgatccgggc cggctacagc atcgccggcc cgcacaccac cgagtcgctc
     4081 ggcgcgtggg acttccggcc cgacctcgcg cgggtcgccg ccccgacgct gatcgtccac
     4141 ggcgaggacg acgcgatccc gatggacctg gtcgcggagt ggacctcggc gctgccccac
     4201 gcccggctga tcaaggtgcc cggcgcgtcg cattttccgt atgtggagcg gcctgacctg
     4261 gtgtggcccg cgatcgagga cttcctgcgc gcgacgccgc cgtgagctca atcgatggcg
     4321 atccgcaacc cgatcccgag cgcggcaaag ttgtcttgcc ggtgactgct ggggttgtcg
     4381 acccggctgt cctcccgcag ccagaagcgc gacagcgaga ggccgaggat gaccgcggtg
     4441 tcgggcggcc ccagctcgag gccggcgtgg agctgggcga tcgcgttgtg cgggagctgc
     4501 agcgtggttc ccggcggtga ctcgcccggc agggtgacgg tgaacgggtg gtggccgagc
     4561 ggcccgctgt agccgaggcc ggcgcccagc atcggacgga cgtagtggtt cgtcccggcg
     4621 accacgccgt ggacgtcggc cgcgccccag gtgcctgccg ttggcgacag gcccccgccg
     4681 acaccggcgc cgagccccca gtggcggtcc tgcgagtgca gcagcacacc gagccggccg
     4741 gtgtagccgt ttcgatcgcc gccctggccg ttgttggtca catggagtac cccgccatcg
     4801 gcctccaggg cgacgccggg ctcgacggtg tggcgcacgc gcgcatcgcc gccgaccagc
     4861 ctgggcccca gcatctcgcc gatgattgac gcggcatcga gctgcacgtc agagtggccg
     4921 accgccggtg cgctcggcga gtccatcacg aaggtccggg tcggcggggt caccgctcgc
     4981 ggtgcgcacg ccgccacaca gagaatccac aagaatgccc gcatgaccgc gccgcgtcgc
     5041 aagcgccatg ccggcgccta agcggcgaga acgcctggcg ggcgcgccgc gtacgccgtc
     5101 gagcttccgg cggcgatggt gattcgacga cggcgggcgt cgggaatcga ccatgccaag
     5161 agctgccggc ggctacccga accagccgcg gcggcggaag aaccagatct gcagcgcgac
     5221 ggtcacgacg aacacgccgc acagcgccca gaacgcccag tcctcgtgct gcagcggcac
     5281 gccgccgacg ttgacgccga gcaggctgca cacgaagccg agcggcagga acaccgcggt
     5341 gatgatcgcc agcacgtaga gccgctggtt ggtcgcctcg gcgatcttgg actgcagctc
     5401 ctcctgggtg accgccgcgc ggtcgcgcgc cgcgtcgagc tcctcgaccg tgcgggtcat
     5461 ccggtcggcg gactcgacga tccgggcgcg cgcggtggcg tcgagccacg gcagctggat
     5521 ctgtgcgagc ttggcgaacg cctcgcgctg cggcgcgagg aaccggcgca gcgcgatcgc
     5581 acgccggcgg tggtcggcga gctgggcgcg cagctcgccg cgggtctcgg tcagcacctg
     5641 gtcctcgcag gccgcgatcg cgtcgctcag ggtgtcgacc cgggtcgcgg cgtactccag
     5701 cacgcgctcg accagcgacg cggtcagatc ggcgaccccg gccggtccgc ggccgcgatc
     5761 gagctcgccg gcgatcgcct tgatcgatcg cgagcggcgg tggcgcaggg tcaccatgcg
     5821 gtgcggctcg atccaggcgc ggatcgacag catgtcctcg ggcgcggcgc cctcgttgag
     5881 gttgatcgcc cggacgatca gcagcagcgc gtcgccgtgg gccgccgcgc gcggccgcgg
     5941 atcgtcgtcg agcagcgcgt cgcggaccac cggatcgatc gccgagcgct cggcgagcca
     6001 gtgctggacg tccgccaccg tgtagtcgag gttgagccac agcgggccgt cggccggggt
     6061 ccagcgcacg acaccgttcc agtcgaggtg ctcgcagccg ccgcggccgt cgagcaggta
     6121 ggcatgcgcg aggccggtcg gctccggggc ctgggtggac atgccggggg tgtactacgc
     6181 gcgcgccccg gtccgcgcgg gggtggggcc acgggtcggc gattcgacgc agcgacaccc
     6241 tgtccgcggc gcaccggacc gctgtagcat ctcaccgtcg tgagtccgat cgactcgacg
     6301 attccctact acccggtcaa cgacttcggg ccgctcatga agggcctggt gatcggcggc
     6361 gtcggcatcc tccacgtgtt cctcgcccag ttcgcgatcg gcggcggcat gctgatgtgg
     6421 tacttcgagc ggctcggcca gcgcggcctc gaggacgcgc ggacgttcgt cgacggctac
     6481 ttcaaggtcc tcgtgctggt cagcttcgtg gtcggcgcgc tgaccggcgt ggcgatgtgg
     6541 ttcacgacga tccaggtcgg cgcccggacg atcggcctca tgatcgacga gttccactgg
     6601 ctgtgggcca ccgagtgggt gtggttcgcg gtcgagatca ccgcgggcta cgcgttctac
     6661 cgctgcggcc cgcggctcgc cggccgcgcg cggctgcgcc tgctcgagct ctatgcgctg
     6721 gcgtcgtgga tgagcctgtt ctggatcaac ggcatcctgg cctggcagct cacgccgggg
     6781 cgctggctcg acggcggcgg cgtgtgggcc gggttcttca acccgagctt ctggccgtcg
     6841 ctcttgttcc gcaccgcggt gtcgaccacg ctcggcgcgc tggtcggctg catcgtgatc
     6901 aacacgatgg acctgccgcg cgagcgccgc gccgcgctga tccgccgggc cgcgcggttc
     6961 gccgcgccga tcgccgcgat gccggtgctc gcgctgtggt tcctcgcggt gctgcccggc
     7021 gacagccggt cgtggctgct cggcggcagc atggcgatga cgatgttcgt cggcatcgcc
     7081 gccgccgcct cgttcctgat cggcggctac gcgatcgtcg gattgatcgt ccagcggctg
     7141 tacatcaacg gcgcgaccgc gaccctgctg ctcggcctgg cgttcggcgc gaccgcggcc
     7201 ggcgagttcg tgcgcgaggg cgcgcgcaag ccatacaccg tgcgcggcgt gctgtactcg
     7261 acgtcgatcc ggcccgccga ggtcgcggcg ctgcgcgcag ccggcgcgac cgccggcgat
     7321 ccgtacccgc tgcgcgacca ctaccccacc gagcagctcg cgcgcggcgc cagggtcgag
     7381 cgcgcgctgt gcgacgcgtg ccacacgatg cgcggcgcca acgccctcgt cgagctgacc
     7441 cggacctgga ccgacgacca gctccgcctc aacctcgcca agctgcagcg caccaagggc
     7501 ttcatgccgc cgttcgcggg caacgccgat gacgtcgagg cgctggtcca gctgctgcgc
     7561 tgggagcgcg atggccggcc gaaggtcgcc gcggcgccgc cgacgtcgcc cgagaccctg
     7621 gcgcagatcg cgcgctggct cgacgaggcc ggcaccgcct ccgccgggga ggcgtccggg
     7681 gaggttccgt gatgtggccg ttcgacggat cgatcggcag cccggtcgag tcgctgtggc
     7741 tcgcgctcta cctggtcacc tttgccctcc acgccgtgct ggtcagctac gtcgccgccg
     7801 gcgccggcta cgcgctggtc gccgccgcgc gccggtccgc cgatccgatc gccgcgcgca
     7861 cccgcgactg gctgccgttc atgctcggcg ccggcatcac cgccggggtc ggtccgctgc
     7921 tgttcctcca gctgctctac cagcgccggt tctacaccgc caacctgctg atgggcccgc
     7981 ggtggggcgc cgtggtgccc gccctgatcg tcgggttcta cgcgctgtac ctggccaagg
     8041 cgtccgagcg gcgccgccgg ctcgggctcg gcgtcgccac cgcgtgcttc gcgttcgtcg
     8101 cctggtcgtg gaccgagctc caccaggtca tgctcgacga cgccggctgg cgcgcgatgt
     8161 acgccgccgg cgagcggttc cacggcggcg ccgcgatcct gccccggctc gctacctggc
     8221 tcggcgcgat gctcgcgctg ttcgccaccg tc
//
LOCUS       DASVOR010000231         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_177598,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000231 DASVOR010000000
VERSION     DASVOR010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177598"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            343..1098
                     /locus_tag="VF516_05075"
     CDS             343..1098
                     /locus_tag="VF516_05075"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8107077.1"
                     /translation="MDGILAGKLALVTGGGRGIGAAIARALAGAGARVVVCGRTKPEL
                     DAVAREIGGVAMRLDLTNRRETDDMTASVGPVDVLVNNAGAAESAPLDRTTDEIWDRI
                     LELDVTAAFRVTRALVPSMIARKWGRVINVASNAGVSGYGYSTAYCAAKHAMVGMTRA
                     LAIDLARTGVTINALCPGWVETQMADEAVTRIAAKTGRSADEARTQLAAMSPQRRMIT
                     PEEVAHAALMLCGEGARGIHGQALVIDGGAVLK"
     gene            1121..1534
                     /locus_tag="VF516_05080"
     CDS             1121..1534
                     /locus_tag="VF516_05080"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8107078.1"
                     /translation="MSSEEIRVPGWPAPRGYANGRTGRGRAVHVAGQIGWDEHGQLVS
                     AEFVPQFIQALDNVIAVVRAAGGVPADIATMTAFVTDVAAYRSGARDIGAAWRARLGR
                     HFPAMTLVGVTGLVEPGAMVEIQATAYIAEPGDPR"
     gene            1531..2343
                     /locus_tag="VF516_05085"
     CDS             1531..2343
                     /locus_tag="VF516_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEX8107079.1"
                     /translation="MIEPRTFAFELARGAATITLDRPERLNALTFDVYRELAESFEQL
                     SREDDVRAIVITGRGRGFCSGGDQDDIIHHLLGRDTPALLAFTRATGRLIHAMRACRR
                     PIIAAINGTAVGAGAVIACASDLRIAAAAAKFGFIFPQVGLCGADMGAMYLLPRIVGL
                     GHATELLLFGDIIPASRALEIGLVNRVMSDADAAVAMAQDWAERLARGPAFAHAMTKQ
                     MLESEHTMALAAAIEAEAQAQALCMQHPDFAEAHAAFKAKRPAKFRGAPDEE"
BASE COUNT          384 a          985 c          963 g          342 t
ORIGIN      
        1 accctgtaca acgcgcggcg cgacgcgctc gcccgcggcc gcgaggacct ggtcgcgcgc
       61 tacaccgccg agatggccgc cgtggtcgag agcacgagcc aggccgaggt gatcgcgaac
      121 atccgcaagg agctcggcta gagcgtgtag cccgtagcgc ccgggagagc gcccacacgc
      181 tgctcgcggt cgcccccggc aagccgcgtt gacgtctcgc gatcgcgcgc ccccacctgc
      241 tgccaccctc gtcgagcgag tggctgctcg agggacacct gggatcagac agctgcctgg
      301 cgggcgagca taaacgcaag gacgccggta taaccccagg ccatggatgg aatcctcgcc
      361 gggaagctcg cgctggtcac cggaggcggc cgcggcatcg gtgcggcgat cgcgcgcgcg
      421 ctcgcgggcg ccggggcccg cgtcgtcgtg tgcggtcgga ccaagcccga gctcgacgcc
      481 gtggcccgtg agatcggcgg cgtcgcgatg cggctcgatc tcaccaaccg gcgcgagacc
      541 gatgacatga ccgcctcggt cggcccggtc gatgtgctgg tcaacaacgc cggggcggcc
      601 gagagtgcgc cgctcgatcg caccaccgac gagatctggg accgcatcct cgagctcgac
      661 gtcaccgcgg cgttccgggt gacccgcgcg ctggtgccgt cgatgatcgc gcgcaagtgg
      721 ggccgggtga tcaacgtcgc gtccaacgcc ggggtgtccg gctacggcta ctcgacggcg
      781 tactgcgccg ccaagcacgc gatggtcggc atgacccgcg cgctcgcgat cgatctcgcg
      841 cgcaccggcg tcacgatcaa cgcgctgtgc ccgggctggg tcgagaccca gatggccgac
      901 gaggcggtga cccggatcgc ggccaagacc gggcgtagcg cggacgaggc ccggacccag
      961 ctcgccgcga tgagcccgca gcgccggatg atcacccccg aggaggtcgc ccacgcggcg
     1021 ctgatgctgt gcggcgaggg cgcgcgcggc atccacggcc aggccctggt gatcgacggc
     1081 ggcgcggtgc tgaaatgatc gagtcaacga aaggaacacc atgagcagcg aggagatccg
     1141 cgtcccgggg tggccggcgc cgcgcggcta cgccaacgga cgcaccggcc gcggccgcgc
     1201 ggtgcacgtc gcgggacaga tcggctggga cgagcacggc cagctggtct cggccgagtt
     1261 cgtgccgcag ttcatccagg cgctcgacaa cgtgatcgcc gtggtccgcg ccgccggcgg
     1321 cgtgccggcc gacatcgcca cgatgaccgc gttcgtcacc gacgtcgccg cctatcgcag
     1381 cggcgccagg gacatcggcg cggcgtggcg cgcccggctc ggccgccact ttccggcgat
     1441 gacgctggtc ggcgtcaccg gcctggtcga gcccggcgcg atggtcgaga tccaggccac
     1501 cgcgtacatc gccgagcccg gagacccgcg atgatcgagc cgcgcacgtt cgcgttcgag
     1561 ctcgcgcgcg gggccgcgac gatcacgctc gatcgccccg agcggctcaa cgcgctgacc
     1621 ttcgacgtct accgcgagct cgccgagagc ttcgagcagc tgtcgcgcga ggacgacgtc
     1681 cgcgcgatcg tgatcaccgg ccgcggccgc gggttctgct ccggcggcga tcaggacgac
     1741 atcatccacc acctgctcgg ccgcgacacg ccggcgctgc tcgcgttcac ccgcgcgacc
     1801 ggccggctga tccacgcgat gcgcgcgtgc cgccggccga tcatcgccgc gatcaacggc
     1861 accgcggtcg gcgccggcgc cgtgatcgcg tgcgccagcg atctgcggat cgccgccgcc
     1921 gccgccaagt tcggcttcat cttcccgcag gtcggcctgt gcggcgccga catgggcgcg
     1981 atgtacctcc tgccgcgcat cgtgggcctc ggccacgcga ccgagctctt gctgttcggc
     2041 gacatcatcc cggcgagccg cgcgctcgag atcggcctgg tcaaccgggt gatgtccgac
     2101 gccgacgccg cggtcgccat ggcgcaggac tgggccgagc ggctcgcccg cggcccggcg
     2161 ttcgcccacg cgatgaccaa gcagatgctc gagagcgagc acaccatggc gctcgccgcg
     2221 gcgatcgagg ccgaggccca ggcccaggcg ctgtgcatgc agcaccccga cttcgccgag
     2281 gcccacgccg cgttcaaggc caagcgcccg gcgaagtttc gcggcgcccc ggacgaggag
     2341 tagcttcggc tgccggtacc gctactgtac ggcgccggtg agtacgaccc gcgccactcg
     2401 cgacgcggtc gaccatgggc aggccagcaa cgaggccggg ttgcccgtcg ttcgtaccat
     2461 aggcgcagcg ccgcgaggac gccgtgccct tcgtggtgga tactctgggg acggatgcag
     2521 ctgtcgagcg atgacctccc gatgttcttc accgaggccc acgtccggct ggccgagcgg
     2581 ctgcgcgcag cggccccggc gttcgccgcg atcgagttgc ccggcgcgca ccccgatgac
     2641 gccgcgcgcg accgggcggc ggtggcggcg ctgg
//
LOCUS       DASVOR010000232         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_323646,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000232 DASVOR010000000
VERSION     DASVOR010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323646"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..773)
                     /locus_tag="VF516_05090"
     CDS             complement(63..773)
                     /locus_tag="VF516_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107080.1"
                     /translation="MPGSPTTSYLVVALDGAADPHQRTTCSTSSSAGRIVVYSRQAMM
                     TALASVLFTLAGAGPPASPAPEPPAPASPAPEPPPATPPQSFTQEVRFDLGIASAVGL
                     AGVTYSRSVAAGLLVELGIGGGVSGLQLSVMPKLVLGASTSRFVVGVGASVGIPGVEI
                     VTDDGPFGSNENKRVITPWLNAEIGYDFRSTSGASFLVAGGITVALAHGCATGIDSCS
                     ELYGLVLPQIRVGYGHWF"
     gene            1179..1475
                     /locus_tag="VF516_05095"
     CDS             1179..1475
                     /locus_tag="VF516_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107081.1"
                     /translation="MTHSIGTSFAPYHAVMSARAPRWIVEQVKQWVGATKKVPAGFAM
                     FPKDLSHPPKEWAERFFHVTRWTEMPRGGHFAAHEEPELLAEDIRAFFRPLRNA"
     gene            complement(1566..2363)
                     /locus_tag="VF516_05100"
     CDS             complement(1566..2363)
                     /locus_tag="VF516_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107082.1"
                     /translation="MTTASTVDPIGAPEGRVGHLAYGAAVAGLGVLCLLSGDLASVFQ
                     PVPRWMPLRPWLAYACGAVLFAGGLALLARKTTRLAALAVTAIFSVWLLVLNLPNTLA
                     KPAVVGNWEGCGLNMTVVAGGWILLALSSRLPAGRAARLLGGSGVKLARCVYALGIPL
                     VGLAHFLNAREATVYVPAWFPLRIDWVYLTGAGHIAAGLAILFGVLPRLAAVLEAAQI
                     TAFVILAHIPSVYGAPGDRVEWAMLVYAVAIAASAWLAAATLRVPAI"
     gene            complement(2721..>3152)
                     /locus_tag="VF516_05105"
     CDS             complement(2721..>3152)
                     /locus_tag="VF516_05105"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX8107083.1"
                     /translation="DAPAPERATTTVTPRLRAQVFARDHHRCTVPGCRSARNLDIHHI
                     VPQAQGGQHELWNLTLMCSGHHAALHDGLLELHGQAPYDIEVRWVYGPPLPLGLDPEA
                     RQAMIRERIAAIFEGLSVAAGPPRAADPARPSWDGTPAAVP"
BASE COUNT          522 a         1099 c         1108 g          423 t
ORIGIN      
        1 cgccgccagg atcccgagta ccagtatcga tcgacgcatc atcctgcgca tcatcgaagt
       61 catcagaacc agtgcccgta gccgacccgg atctggggca ggaccagccc gtagagctcg
      121 gagcaggagt cgatgcctgt cgcacatccg tgggcgaggg ccacggtgat gccgccggcg
      181 acgaggaacg acgcgccgct cgtggagcgg aagtcgtaac cgatctccgc gttgagccag
      241 ggcgtgatca cgcgcttgtt ctcgttgctc ccgaaggggc cgtcatcggt cacgatctcc
      301 acgcccggga taccgaccga cgcaccgacg cccaccacga agcggctggt cgatgcgccg
      361 agcacgagct tgggcatcac cgaaagctgc agtcccgaga cgccgccccc gatgccgagc
      421 tcgacaagca atccagcggc caccgaccgc gagtaggtga cgccagcgag accgaccgcc
      481 gaggcgatcc ccaggtcgaa gcgcacctcc tgggtgaagc tctgcggcgg cgtggccggg
      541 ggcggctcgg gggccggcga ggcgggggcc ggcggctcgg gggccggcga cgcggggggc
      601 ccggcgccag cgagtgtgaa cagcaccgat gcgagcgcgg tcatcatggc ctggcgcgag
      661 tagaccacga tgcggccagc gcttgaggaa gtcgagcacg tcgtcctttg gtgcgggtcc
      721 gccgcgccat cgagcgcgac gacgagatag ctcgtcgtcg gcgagccggg cacgacgcgg
      781 atccagcccg ccgcttcgcg gtggcatggt gcgtgctctt ggccattcta tgacccgaca
      841 actcgcgacg aagatcgatg aggtcgcacg accatttcgc atcgcgcttg acgagcacgt
      901 catcgaggac ctgaaggccc gcctggagcg cacgcgctgg ccggtgagcc ccgatgggct
      961 ggcgaccacg tgggaagcag gcacgagcca cgcctacctg cgcgagctgg tcgactactg
     1021 gcgcacgacg ttcgactggc gtgcgcgcga gcgcgagctg gacaggttcg cgcatcatac
     1081 gatctcgctc ggtgccggca ccgtgcactt catccacgcg gtcgggcgtg gccctgcacc
     1141 gctgccgctc gtgctgacgc acgggttccc tgtactgggt gacgcactcg atcggcacgt
     1201 cgttcgcgcc gtaccacgcc gtcatgtccg cgcgcgcgcc gcgctggatc gtcgaacaag
     1261 tcaagcagtg ggtcggcgcg acgaagaaag tgcccgccgg cttcgcgatg ttccccaagg
     1321 acctctcgca tcctccgaag gaatgggcgg agcggttctt tcacgtcacg cgctggaccg
     1381 agatgccgcg cggtggtcac ttcgccgcac acgaggagcc ggagttgctc gcggaagaca
     1441 tccgcgcgtt tttccgaccg ctgcggaacg cgtgagtcac cgacgtgggc gctgcggggc
     1501 cgccggggcc agtggcgcag ctcgatgtga gtcccagcca gacagcccag gccttcctat
     1561 ctggactata tcgccggtac gcgcagggtc gccgcggcga gccaggcaga tgcggcgatc
     1621 gccacggcgt agacgagcat cgcccactcc acccggtctc ccggcgcacc gtagacggac
     1681 gggatgtgcg cgaggatcac gaacgccgtg atctgagcgg cctcgagcac cgccgcgagc
     1741 cgcggcaaga cgccgaacaa gatggccagc cccgccgcga tgtgccccgc gccggtcaag
     1801 tacacccagt cgatccgcag cgggaaccac gccggcacgt agacggtcgc ctcgcgagcg
     1861 ttcaggaagt gcgcgaggcc gacgagcggg ataccgagag cgtagacgca ccgcgcgagc
     1921 ttcaccccgc tccctccgag caggcgcgcg gcgcggcccg ccgggaggcg actcgacagg
     1981 gcgaggagga tccagccccc ggcgacgacg gtcatgttca agccgcagcc ctcccagttg
     2041 cccaccaccg ctggcttcgc aagggtgttc ggtaggttca agacgagcag ccagaccgag
     2101 aagatggccg tgaccgccag ggccgcgagc cgcgtcgtct tccgggcgag cagcgcgagg
     2161 ccgccggcga acaggacggc tccgcatgcg tacgcgagcc atggccgcag cggcatccac
     2221 cggggaacgg gctgaaagac agaggcgagg tcaccggaga gcaggcacag cacgcccagc
     2281 ccggccacgg ccgcgccata cgccaggtga ccgacgcgcc cttcgggcgc accgatcggg
     2341 tcgactgtgc tcgcggtggt cacgcgttgc ctcgacccgg aagctacatt ccgggcggga
     2401 gtgggtccac tggcacagga tctcccgggg gctcgatcgg cactgcctcg cgcatcatgt
     2461 gcgacgggtc gtgcacggac gccggtgcag tttcggacgc gccggcccac gcctcacttc
     2521 caggccgacg atgcacccga acggggccgg ccgtcaggct gcgatcgcgc ggcgccacga
     2581 tacacgcgac gacatctcac tccaccatcg cccagctgac gaaggccgcc gagcaagtgc
     2641 gctcgcggat catcgcctat cgtgccaggc ggcctgcctg gctgaaggcg ccgtgacggg
     2701 cgtcttgccg agagacggtc tcaggggaca gctgctggcg taccgtccca gctgggacgc
     2761 gcgggatccg ccgcccgcgg gggcccggcg gccactgaca gcccttcgaa gatcgccgcg
     2821 atgcgctcgc ggatcatcgc ctgccgcgcc tcgggatcga gcccgagcgg cagcggcggc
     2881 ccgtacaccc accggacctc gatgtcgtac ggtgcctgcc cgtgcagctc cagcagacca
     2941 tcgtggagtg ccgcgtggtg gcccgagcac atgagtgtga ggttccacag ctcgtgttgg
     3001 ccaccctggg cctgcggcac gatgtggtga atgtccaggt tgcgggctga acggcagccg
     3061 ggcaccgtgc agcggtgatg gtcgcgcgcg aacacctgcg cgcgcagccg cggcgtcacc
     3121 gtggtcgtcg cgcgctccgg cgccggcgca tc
//
LOCUS       DASVOR010000233         5178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_469802,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000233 DASVOR010000000
VERSION     DASVOR010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469802"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VF516_05110"
     CDS             <1..386
                     /locus_tag="VF516_05110"
                     /inference="COORDINATES: protein motif:HMM:NF040046.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6285 domain-containing protein"
                     /protein_id="HEX8107084.1"
                     /translation="PAIQPDAPALLDAAIDYLERELLPTLAGYHRFQCRVTINVLAQV
                     RRELALAPHQADVERARLVALLGHPGERDDLSRELAARIRAGDIALDDPALLDHLRQS
                     LVEALRINNPKWIGEPDTPGAPERG"
     gene            complement(395..835)
                     /locus_tag="VF516_05115"
     CDS             complement(395..835)
                     /locus_tag="VF516_05115"
                     /inference="COORDINATES: protein motif:HMM:NF037830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107085.1"
                     /translation="MPRRPPDPLVYYLDANLDGPELVRRLRDAGMPCEPHRDHFAPDA
                     EDEDWIPTIASRGWIIVTRDFAIKRRPNEREAWTVANATVVMVRGERLSAEDMSRLLL
                     TAHAQGRLDNYIAKRTPPMILYLNPDGQLHTHLGGERRGGRKKI"
     gene            complement(816..1220)
                     /locus_tag="VF516_05120"
     CDS             complement(816..1220)
                     /locus_tag="VF516_05120"
                     /inference="COORDINATES: protein motif:HMM:NF016167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX8107086.1"
                     /translation="MGGDRPLLATPFRTDGRGIFIEQMGKLVDAAHGGQAAIREVVES
                     SLERVELDERRLPLRLYPWRREPTEPRIIALDPRRSFGKPTVVGSAVQSETIIDRHRA
                     GESVAQLAADYNLPGEVIEGVLRWGLDAAKAA"
     gene            complement(2922..3332)
                     /locus_tag="VF516_05125"
     CDS             complement(2922..3332)
                     /locus_tag="VF516_05125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107087.1"
                     /translation="MPILIEEVEPFNDIDWTELGIVGVEPSLEIATLAAQRRAGLSFH
                     DHLCLLLARDNGWTCVTNDGRLRRECAAEGVSTLWGLEVVALLVEDAVLAVSAAEDIG
                     RAIQAANPKFITGPDPRLLPEADRRSRVATEALI"
     gene            complement(3458..3994)
                     /locus_tag="VF516_05130"
     CDS             complement(3458..3994)
                     /locus_tag="VF516_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107088.1"
                     /translation="MAPRLFALDHNFPQPVLAALADALPQVELVPIRDIDPVFADLDD
                     WELLRELNRHERPWDGMITNDDAMLWLPKEMTVLSQTGLTLVVAKGEGHNPIRAVGTL
                     LCHLTHICHHTTPGIPQIWRLRVAQKNAEPVHDYLETIAERSSTAVDKQMISVGRAAE
                     ILGVDLRTMHEHVASWVD"
     gene            complement(4005..4667)
                     /locus_tag="VF516_05135"
     CDS             complement(4005..4667)
                     /locus_tag="VF516_05135"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HEX8107089.1"
                     /translation="MDAVGAYTADRAAALSGVPKSTIRWWARHDMLVPSVSSTRVMLW
                     SYADLMGLRVIYWLRQRKTTQLGVEIPRTSMPAIRRALNELRKLELSLWDQDRPTLLV
                     DGNGHVYLKRSERVQDLTGQVVGEPLLDLISPFTTAEGTRGPDLIKPRVDLRIVPGKL
                     SGAPHIVGTRLETRALAALATDGFDVTAIHRLYPYVSAPQIEQALELEDQLADNLSKR
                     AA"
     gene            4733..>5178
                     /locus_tag="VF516_05140"
     CDS             4733..>5178
                     /locus_tag="VF516_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107090.1"
                     /translation="MVTRYPLAPMDSLLRVLTWICVAIPLVVGLAAIRAPAPARAILF
                     GVAGAMLAIYAFIWLWMRPTAFVVVPDGLVIDWPVHRRRIETAAVTAARILGRDQLCR
                     ELGRIVRIGAGGLGGGFGLARTARGLVELWVSRTDWMVLVECTGRRS"
BASE COUNT          839 a         1817 c         1644 g          878 t
ORIGIN      
        1 tgccggcgat ccagcccgac gcccccgcgc tgctcgacgc cgcgatcgac tacctcgagc
       61 gcgagctgct accgaccctc gccggctatc accgcttcca gtgccgggtc acgatcaacg
      121 tcctggccca ggtccgccgc gagctcgccc tggcgcccca ccaggccgac gtcgagcgcg
      181 cccggctcgt cgcgctgctc ggccaccccg gcgagcgcga cgacctgagc cgcgagctcg
      241 cggcccggat ccgcgccggc gacatcgccc tcgacgatcc ggcgctgctc gatcacctgc
      301 gccagtcgct cgtcgaggcg ctgcggatca acaaccccaa gtggatcggc gagccggaca
      361 cccccggcgc gcccgagcgg ggctgatcaa gccgctatat cttcttccgg ccgcctcgcc
      421 gttctccacc aaggtgcgtg tgcagctgac catctggatt caggtagagg atcatcggtg
      481 gcgttcgctt cgcgatgtag ttgtcgagtc gtccctgggc atgggcggtg agtagcaacc
      541 tcgacatgtc ctccgcgctc agccgctcgc cgcgtaccat gacaaccgtc gcattcgcca
      601 ccgtccatgc ttcgcgttca ttcggtctgc gcttgatcgc gaagtcgcgg gtgacgatga
      661 tccagcctcg cgacgcgatc gtcgggatcc agtcctcgtc ctcggcgtcg ggcgcgaagt
      721 gatcgcgatg cggctcgcac ggcatgcccg catcgcgcag tcgccggacg agctctggac
      781 cgtcgaggtt cgcgtccaga tagtaaacca gcgggtcagg cggccttcgc ggcatcgaga
      841 ccccaccgca acacaccctc gatcacctct cccgggaggt tgtagtccgc cgccagctgg
      901 gccacggatt caccggcccg gtgacgatcg atgatcgtct cgctctgcac cgcgctgccg
      961 accaccgtcg gttttccaaa cgaccggcgc ggatcgagcg caatgatcct cggctcggtc
     1021 ggctctcgcc gccacggata gagccgaagc ggaagccgac gctcgtcgag ctcgacgcgc
     1081 tccaggctgc tctccaccac ctcgcggatc gcggcctggc cgccatgcgc ggcgtcgacc
     1141 agcttcccca tctgctcgat gaagataccg cggccgtcgg tgcgaaacgg cgtggcgagg
     1201 agtggacggt cgccgcccac gaaccgcagc gcgcttcgca ctcgctggag cggcagatcg
     1261 aagcgccgtg tcaacgacgc caggacgtac gcctccatca ggttgttgaa cgtgaggagc
     1321 cctgtcgcac ggtcgggctt gatcagacca tgcatccgtg tccgcccgcg gacacgctgg
     1381 cctcgaaccc aggtcgccag ggtcgacctg ggcacgccca ggtacaccgc cgcgtcgccg
     1441 atcccgtaca tcggcagctc gcgcggatcc ttcccgcgat agaggctgac gacctttccc
     1501 ataccccaca cgtaccgcca tgtggaggtc gacgcaacta gcgagccgag tggagatctc
     1561 ctcgatccaa gtccgcggaa agatcaatgt ctgcccgccg cggaacgcca gcccggcgac
     1621 gccgtcgctt ttcgccggcg cacgcccgcc gccccggcga tcgcgccgcc ctcggcactg
     1681 gtgagcgcgg agggtgtggg ccgccgaagc acgggacatc catcggtggt cagaccgcac
     1741 cgccgcctcc ccggatcgtc cgcggactcg ggcttgccaa tcacttcgac aggcaaggga
     1801 tgaagcaccg ccgtcccacc acgtccacga cgccccgccc gtccgctcat gcccgccgag
     1861 ctccgcgccg gcagcgacgc gatcttctcg tcgatgtgcc ggccgcgacg cagcgccggc
     1921 cgcgccagga acccgccgtc gtgcctcgcc gccgtcgccc ggcggcagca gcaccgacag
     1981 gcccatcgac gccggcagca ccgaccgacg gcgagacccc gcgccagccg gccggacatc
     2041 ctccgcgccg gcgtcttcct cctcccctcg gcatcctcgg tttccgtgtc gtcctccggc
     2101 tcgatgatcg attcctcgct ccgcccctct cgcggcacga ggaacccggt caggtagaac
     2161 cgcgacggcg cccgatcgag ctcctcctcg gggtcttcgg gcttgctcga tctcccggtc
     2221 acgtatcgga tcaggttcca tgggggccag atcgcagagc tccatggcag cacggaacga
     2281 cgactcgggc gagatcggac cttcatggcg catcaactcg aggttgcgac gctcagcagc
     2341 gcggtgtcca gcgatccagc gacggacggc ttctttgtcc acgacgcgac ctagcatcct
     2401 gctggagcag ttgacgatgc ttcgtcggtc aagcgtggtt tgagatcatc atgagggtac
     2461 ggtgaccgag attctggccg gtgcacggcg agtcacttga cggacttaca caacgactcg
     2521 gacgatggac gagacacgaa aaatgccagc atggccttga tcctcatctt cgctttcgcg
     2581 gcgcgctccc tggccaactg tgctcctgcc agtggcgcac gcatgtccag ctgggctagc
     2641 aacggaacgc agggagacgc cggtgccgtc cacatcgtga acttgtcctg gtccggtatc
     2701 ttcgccaccg ccacgtggat accaggtgca aggatcgcct tgcaacctca tcttgcatga
     2761 gcctggctgg actgcaggcg acctgcgcaa cgtcacccga gatgaaccgt catggctcat
     2821 gacagtagac ttatcatcgt aatggaaatc gttaacctgt aacctcggac gtctccaggt
     2881 gcgagcgcca gtcggcggga tcgcgtgggt cttgtcggca gctaaatcag cgcttcggta
     2941 gcgacccggg agcgccgatc cgcttcagga agcagtcgag gatccggccc ggtgataaac
     3001 ttcggattcg ccgcctggat cgccctaccg atgtcttcgg cagccgagac tgcgagcacg
     3061 gcgtcctcga ccagcagcgc gacaacttcc agcccccaca gggtcgagac accctccgcc
     3121 gcgcactcgc gccgcaggcg accgtcattg gtgacgcatg tccatccgtt gtcgcgcgcg
     3181 aggagcagac acaggtgatc gtggaacgac agcccggcgc gccgttgtgc cgccagcgtc
     3241 gcgatctcga gcgatggctc gacaccgacg atccccagct cggtccagtc gatgtcgttg
     3301 aacggctcga cttcctcgat gaggatcggc aagggtcgtg gacgcgcccg atgtgcttcg
     3361 agatcagcgg gatgacggtg cgatcggagt cgcagaagtc gatcaggacg tttgcgtcca
     3421 tcaggagcag cgtgcgacgc gagcttgcca tggcacgtca atcgacccag gatgccacgt
     3481 gctcgtgcat cgtacgcaga tcgacgccga ggatctccgc cgcgcgaccc accgagatca
     3541 tctgcttgtc gaccgcggtg ctggatctct ccgcgatcgt ctcgaggtaa tcatgcaccg
     3601 gctctgcgtt cttctgtgcg acccgcagcc tccagatctg cggtatcccc ggggtcgtgt
     3661 gatggcagat gtgagtcagg tggcagagca gcgtcccgac cgcgcggatc gggttatggc
     3721 cttccccttt ggcaaccacg agcgtcagcc cggtctgtga cagcaccgtc atctccttcg
     3781 gtaaccacag catcgcatcg tcgttcgtga tcatcccgtc ccacggtcgc tcatgtcgat
     3841 tcagctcgcg caagagctcc cagtcgtcga gatcggcgaa caccggatcg atgtcgcgaa
     3901 tcgggacgag ctcgacctgc ggcaacgcat cagccagcgc cgcgagcacg ggctgaggaa
     3961 agttatggtc gagcgcgaac aggcgaggtg ccacgccgtc gacctcaagc cgcgcgcttg
     4021 ctcaggttgt cggcgagctg gtcttcgagt tccagcgctt gctcgatctg cggcgcgctc
     4081 acgtacggat acaatcgatg gattgccgtc acgtcgaagc catcggttgc cagcgccgcg
     4141 agcgcgcgcg tctcgagccg cgtgcccacg atgtgagggg cgccgctgag cttgcccggg
     4201 acgattcgca gatcgacacg cggcttgatg agatccggtc cgcgggtgcc ctccgcggtg
     4261 gtgaacggcg agatcagatc gagcaacggc tcgcccacga cctgcccggt gaggtcctgc
     4321 acccgctccg agcgcttcag gtagacatga ccattcccgt ccacgagcag cgtcgggcgg
     4381 tcttggtccc agagcgacag ctccagcttc cgcagctcat tcaaagcccg tcgaatggcc
     4441 ggcatgctgg tgcgcgggat ctcgaccccg agctgggtgg tctttcgctg ccgtaaccag
     4501 tagatgactc ggagccccat gaggtcggcg taggaccaga gcatcacccg cgtggaggat
     4561 acgctcggga cgagcatgtc gtgccgtgcc caccagcgga tcgtggactt gggcacgccc
     4621 gacagcgccg ccgccctgtc tgccgtgtat gcgcctaccg catccatacg agggagccta
     4681 gcacggcggc gatgcacgga ctcggcgcct ggcgccatgg cacgctggag agatggtgac
     4741 acgatacccg ctcgcgccga tggacagcct gctccgcgtg ctgacctgga tctgcgtcgc
     4801 gatcccgctg gtcgtcgggc ttgcggcgat ccgtgccccg gcgccggcac gcgcgatcct
     4861 gttcggcgta gccggcgcga tgctcgcgat ctatgcgttc atctggctgt ggatgcgccc
     4921 gaccgccttc gtggtagtgc ccgatggcct cgtcatcgac tggccggttc accggcgccg
     4981 gatcgagacc gccgccgtca ccgccgcccg gatcctcggt cgggatcagc tgtgccgcga
     5041 gctgggccgg atcgtcagga tcggggcggg aggcctcggc ggtgggttcg ggctggccag
     5101 gaccgcgcgc ggtctcgtcg agctgtgggt ctcgcgcacg gactggatgg tgcttgtcga
     5161 atgcaccgga cgacgatc
//
LOCUS       DASVOR010000234         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_352924,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000234 DASVOR010000000
VERSION     DASVOR010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_352924"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..743)
                     /locus_tag="VF516_05145"
     CDS             complement(234..743)
                     /locus_tag="VF516_05145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107091.1"
                     /translation="MTTNIANPADNLRAALKQAGFNARKVSVRQHHSTLCVTLRDASA
                     SLSRVTEIAGQFEQVRRDHATGEILCGGNTFVRVEYADALVDPIKAEILAVLDPAPND
                     EYIALPGGFRACKVSSQHGATAYVREVRMEGPTFHDRNSIAVGVSWAAERLAVARLDA
                     SALAQGAAS"
     gene            complement(1232..1723)
                     /locus_tag="VF516_05150"
     CDS             complement(1232..1723)
                     /locus_tag="VF516_05150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107092.1"
                     /translation="MAAIIGATAGLVPVGVYEVGHHELTSWSPIECPKAMIVYAGLLF
                     SALTVLGWSTNMFGARAKALGFTLLLEGIMMTSSAGWLTAIALTYLVLINAVANGCRL
                     ALRDGEPAGIDWPSNLAQAIAVAAGATAAQSKKPTRARRSRRTGSRKTQPRLAVAAPV
                     YWS"
BASE COUNT          312 a          605 c          618 g          290 t
ORIGIN      
        1 gtcaagctct cgtcgacacc gcagcctcac cacgtcgcaa ctgcacgtaa ccgggcgctt
       61 cacataaaag aacctcgatc ggcgcgccgg tagctcgccg cacacgacac actccccgat
      121 cccatggtcc tccaactcgg cgatcagcgc gaccgaacgg gtctgaccga cgtacgccat
      181 catcgccacg cctcccccgc tcaccgtgcc agatggcgag gtcgacgctg cgctcacgac
      241 gcggcaccct gcgccagcgc gctcgcgtcc aggcgcgcga cggcgagccg ctcggcggcc
      301 cagctgacac ctacagcgat gctgttgcgg tcgtggaacg tcggcccctc catgcgaacc
      361 tcccgcacgt aggcggtcgc gccgtgctgg ctcgacacct tgcacgcgcg gaagccgccg
      421 gggagcgcga tgtactcgtc gttgggggcg ggatcgagca ccgcgaggat ctcggccttg
      481 atggggtcga cgagcgcatc ggcgtactcg acgcgcacga atgtgttccc tccgcagagg
      541 atctcaccgg tcgcgtgatc gcgcctgacc tgctcgaact gaccggcgat ctccgtgact
      601 cgggacagcg acgcggacgc atcgcggagg gtgacgcaga gcgtgctgtg gtgctggcga
      661 acgctcacct tgcgtgcatt gaaaccagct tgcttgagcg cggcgcggag gttgtcggcg
      721 gggttggcga tgttggtggt catatgcaga atggcattgc gcaacaagtg ccagttttgc
      781 gagctccaaa catcgccaca ttgctgcgat ggtctctcgg tagctcgccg acctgtggcg
      841 caaggacaac gatcctggcc aggtgagttg ccacaacgac tcacgaacta tccattgcgc
      901 aacttgacca ggcagagcag tgaagcgagc cagattgaat gccgatgagc tcgataggca
      961 accatgagct caccgagacc ttgtgagctc ggcggcacac catcgcgggg actgttgcac
     1021 tgatcatctc cgaaggatgc tatcccgggc tcaggcgccg ggcctggcgg ggatcggaac
     1081 cgcgtcgcca tttgactcca tgggtagcgg cgcagtggtg agctccggcg gagcatggcg
     1141 cacgatgcgt caagtcgcgc cgagctcttg gctggcgacg cgcgctcggc gcgtccggcg
     1201 gcgtcggggc gcttggaccg cgccccgcga ctcaagacca gtacaccggc gcggcgaccg
     1261 cgagccgagg ctgcgtcttg cgggatccgg tgcgccgcga gcggcgtgcc cgggttggct
     1321 tcttggactg cgctgccgtc gcgcccgccg caacggcgat agcctgcgcc aggttcgacg
     1381 gccagtcgat cccggcgggt tccccatcgc gcagcgcgag ccgacagccg ttcgccaccg
     1441 cgttgatcag caccaggtag gtgagcgcga tcgcggtgag ccatccggcg ctcgacgtca
     1501 tcatgatgcc ctccaggagc agcgtgaagc cgagcgcctt ggcccgcgcg ccaaacatgt
     1561 tcgtggacca accgagcacg gtgagcgccg agaacaggag cccggcgtac acgatcatgg
     1621 ccttggggca ttcgatgggt gaccacgacg tcagctcgtg atggccgacc tcgtacacgc
     1681 ccacaggaac gagcccagca gtcgcgccga tgatcgcggc caccggcttg cgccgggcag
     1741 cgcgcagctg ggcgagcggg ttggtggtgg cgggatgttc ggaagcagag gtgcagatcg
     1801 tcgtcatgtg ccgagtggca ctgcg
//
LOCUS       DASVOR010000235         3322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_177604,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000235 DASVOR010000000
VERSION     DASVOR010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177604"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VF516_05155"
     CDS             <1..1345
                     /locus_tag="VF516_05155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX8107093.1"
                     /translation="CVVNRCGDGVLDMNGTHTEECESAIVQPANSRALTSPESQTCNN
                     DCTTPSCGDGKVNRKFTPTGAAGPEQCDVVDATGNSLDSDDRDCTAHCQINVCGDGLH
                     DTKGPNHIEPCDDGNKIDADGCSNACTLPSCGNGIVDQGEECDDGNTTNGDACENNCT
                     LPRCGNGIKDVGEDCDDGNSLNSDDCTNACKFATCGDGFIRTVLTPPPVGTPTVVEQC
                     DFALGPTACVYDPVSKPCSICDTTCQLVPTVAPFCGDGHTDTPFETCDSGNPTCGACT
                     SDCQVVTSQKATGLIFAAAGKDLRANDTFKLSDGFTTPVTFEFTQTTVNGAGNVAIIF
                     DPGDPTDPTKPGDSNSDMAAKIRDAINGARAGGLEISAVRLGSIVQLTNDRASSLGNG
                     TLGDPRIDPHVQTSNFGVVDMSGGLGGNCATGTPCAINEDCASGSCSTTSPHVCQ"
     gene            complement(1366..2889)
                     /locus_tag="VF516_05160"
     CDS             complement(1366..2889)
                     /locus_tag="VF516_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HEX8107094.1"
                     /translation="MGIRLAISLALLMWHSVAHAVSFTVTNTEDAGPGSLRQALINAS
                     NGGTNTILISATGTIALASPLPMASGTLTISGPGADKLTIQGSPGPAPVLSSTATLQV
                     SGVTITGGANAAGNGGGIQVMGGSLTLLNSTITGNTAALGGGIYSTVGSLTILSSTIS
                     GNTGTGAIYSGNDISVFDSTIADNQGTAIISTTAGSILSIDRSTISGNTDAAGIGGIQ
                     LQGGTANIRNTTFSGNSGPQGGDFWTSSDGVSLKLINVTAIGGSAPALLFDHAATVML
                     RNTLLAGTGTRCSAGHLPTSQGHNLSSDTTCNLTQTTDKPGVDPLLGPLAANGGPSKT
                     HAPLAGSPVLNAGDGAGLETTDQRGVPRIQFAAVDIGAVEVSEPMVVTQPMPQTVAEG
                     DMFTLSVAAMNQNSTTPLKYQWRKDGTAIAGATGATFTNPAAAPEDAGMYDVLVINDG
                     GGLPSMAVAVNVTPAPKPEPASGGCCSSTADGAAWTDAVLGLGLVALLGVPRRRRAR"
     gene            3168..>3322
                     /locus_tag="VF516_05165"
     CDS             3168..>3322
                     /locus_tag="VF516_05165"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8107095.1"
                     /translation="MQLSSLKVIITGAAQGMGAHFAHRLAEAGAQVAAGDVKEDGLAA
                     LAESTRGL"
BASE COUNT          554 a         1136 c         1113 g          519 t
ORIGIN      
        1 ctgcgtggtc aaccgctgcg gcgacggcgt gctcgacatg aacggcacgc acacggagga
       61 gtgcgagtcg gccatcgtgc agcccgccaa ctcgcgcgcc ctgacctccc ccgagtcgca
      121 gacctgcaac aacgactgca cgaccccgag ctgcggcgac ggcaaggtca accgcaagtt
      181 caccccgacc ggcgcggccg gacccgagca gtgcgacgtc gtcgacgcca ccggcaactc
      241 gctcgacagc gacgacaggg actgcaccgc gcactgccag atcaacgtgt gcggcgacgg
      301 actgcacgac accaagggcc cgaaccacat cgagccgtgc gacgacggca acaagatcga
      361 cgccgacggc tgctcgaacg cgtgcacgct gccgagctgc ggcaacggca tcgtcgacca
      421 gggcgaggag tgcgacgacg gcaacaccac caatggcgac gcctgcgaga acaactgcac
      481 cttgccgagg tgcggaaacg gcatcaagga tgtcggcgag gactgcgacg atggcaactc
      541 gctcaactcg gacgactgca cgaacgcgtg caagttcgcg acgtgcggcg atgggttcat
      601 tcgaaccgtg ctgaccccac cgcccgtggg gacaccgacg gtggtcgagc agtgcgattt
      661 cgcccttggc ccgaccgcct gcgtctatga tccggtctcg aagccatgca gcatctgcga
      721 cacgacctgc cagctcgtcc cgacggtcgc tccgttctgc ggcgacggcc acacggatac
      781 cccgttcgag acctgcgact cgggcaaccc cacctgtggc gcatgcacct cggattgcca
      841 ggtcgtgacc tcgcaaaaag ccacgggctt gatcttcgcc gcggccggga aggatctccg
      901 cgccaacgac acgttcaagc tgtccgacgg cttcaccacc ccggtgacgt tcgagttcac
      961 ccagaccaca gtgaacggtg cggggaatgt cgcgatcatc ttcgatcccg gcgatcccac
     1021 cgatcccacc aagcccggcg acagcaacag cgacatggcc gccaagatca gggacgcgat
     1081 caatggtgca cgggccggtg ggctcgagat ctccgcggtc cgcctcggca gcatcgtgca
     1141 gctgacgaat gatcgagcca gctcactcgg gaacgggacc ctcggcgacc cgcggatcga
     1201 tccgcatgtt cagacctcca acttcggcgt ggtcgacatg tccggcggcc tgggcgggaa
     1261 ctgtgcgacc ggcaccccgt gcgcgatcaa cgaggattgc gcatcgggga gctgctccac
     1321 gaccagcccc cacgtctgcc agtagaccgc gtcaccggcg ccggttcagc gggcgcgtcg
     1381 ccggcgcgga acgccgagca atgccaccag ccccagcccc agcacggcat cggtccatgc
     1441 ggcgccgtcg gcggttgacg agcaacagcc gcccgaggcc ggctcgggct tgggcgcggg
     1501 cgtcacgttg accgcgactg ccatgctggg aagtcccccg ccatcgttga tcacgagcac
     1561 gtcgtacatg ccggcgtcct cgggcgctgc ggcggggttg gtgaaggtag cgccggtggc
     1621 accggcgatg gccgtgccat ccttgcgcca ctggtacttg aggggcgtcg tgctgttctg
     1681 gttcatcgcg gcgacgctga gcgtgaacat gtcgccctcg gcgacggtct gtggcatggg
     1741 ctgcgtcacg accatcggct cggagacctc gacggcgccg atgtcgaccg ccgcaaactg
     1801 gatccgcggc acgccgcgtt gatcggtcgt ctcgagtccc gcgccgtccc cggcgttgag
     1861 cacgggactg ccggcgagcg gcgcgtgcgt cttgctcggg ccgccgttcg cggcgagtgg
     1921 accgagcaag gggtcgacgc ccggcttgtc ggtggtctgg gtgagattgc acgtcgtgtc
     1981 gctcgacagg ttgtggccct gcgaggtggg gagatggccc gccgagcagc gcgtccccgt
     2041 tcctgccagc agcgtgttgc gcagcatgac cgtcgcggcg tggtcgaaca gcagcgccgg
     2101 tgcgctgcca ccgatcgcgg tgacgttgat cagcttcagc gagacgccgt cgctggaggt
     2161 ccagaagtcc cctccctgcg gtcccgagtt cccggagaac gtggtgttgc ggatattcgc
     2221 ggtgccgcct tgcagctgga tccctccgat cccggccgcg tccgtgttgc ccgagatcgt
     2281 gctgcggtcg atcgagagga tcgagcccgc ggtcgtcgat atgatcgcgg tgccctggtt
     2341 atccgcgatc gtcgaatcga acaccgagat gtcgttgccg ctgtagatcg ccccggttcc
     2401 cgtgttgccc gagatcgtgc tgctcaggat cgtgagcgat ccgacggtag agtagatgcc
     2461 gccgccgagg gctgcggtgt ttcccgtgat cgtgctgttg agcaacgtga gcgagccgcc
     2521 catcacctgg atgccgccgc cgttgccagc cgcgttggct ccgcccgtga tcgtcacgcc
     2581 cgacacctgc agggtagcgg tgctcgacag cacgggggcc ggccccggac tgccctggat
     2641 cgtgagcttg tccgcgccgg gaccgctgat cgtcaacgtg ccgctcgcca tcggcagcgg
     2701 cgatgcgagc gcgatcgtcc cggtcgcgct gatgaggatc gtgttggtgc ccccgttgct
     2761 cgcgttgatc agggcctggc gcaacgaacc cggtcccgcg tcctccgtgt tcgtgacggt
     2821 gaacgacacc gcatgcgcga cggaatgcca catcagcaat gcgaggctga tcgccagacg
     2881 aattcccatg ccgctacgct agaccaggct cgtgcaacat gcatccatcg ccgagggccg
     2941 cgcgaccgag gaagccgcgc gatctgcagg tcgtgcgaag tgtaccaatg gccgagcccg
     3001 gctgatatat ccgacactgc gcgcctccgc tcgaggccga cgaccacccc ggaatggccg
     3061 gaatggccgg aatggccgga atggtgtgcc gagaaatttc tgcgcgcccg gttcgaaggc
     3121 ggctggtgaa ttctaccccg gaaggcaagg aaggataatt tccccccatg cagctgtctt
     3181 cgctgaaggt catcatcacc ggggccgccc agggcatggg cgcccacttc gcgcaccggc
     3241 tcgccgaggc cggcgcgcag gtcgccgccg gcgacgtcaa ggaggacggc ctggccgcgc
     3301 tcgcggagtc gacccgtggg ct
//
LOCUS       DASVOR010000236         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_235877,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000236 DASVOR010000000
VERSION     DASVOR010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_235877"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VF516_05170"
     CDS             complement(<1..1356)
                     /locus_tag="VF516_05170"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEX8107096.1"
                     /translation="MSSSEQVGRQTRRGISWSFAGAVATNGMRLVVVTVLGRALTSTD
                     FGIVVAAVSVNVVVYGIRDVGIGLALVQRKQLEPGHLTTAFAVSVYLGVALTALLWLA
                     APLIGRFYGIPESVDVIRVLAVVFFGLRSLATTSRMLCQRAMNFRRIAIVDAAAFLTG
                     SVVSIACALAGLGPWALVAGYLIEELIATALYLRLSPPRYSLAVDRARLRELLRFGAG
                     QSAIQSTNVVATYGDNFVVGHVLGATHLGYYSRAYDLIKFPSMVFDAIVGNVLFPAFA
                     RLQDDRRALASHLRRTAFVNALVLLPASAAIIVLAPEAIRLLMGPNWGEMVLPFRILS
                     VTILLRTNLKLGGILAQAAGAVNTVAAAYVVYMVVVVGGALITVRWGIAGVAVSTALA
                     ITTVSLHCCLLAMRCSELPPGSFLAAHVPGAVLAAAVTAVGWPLAQALRAAGLPFALV
                     LA"
     gene            1460..>2166
                     /locus_tag="VF516_05175"
     CDS             1460..>2166
                     /locus_tag="VF516_05175"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8107097.1"
                     /translation="MTERRIRILFAIPALDKGGPDRVFFELLRALDRARFSPVLVTSE
                     PTGHYLSRLPGDVELHHLGREVGIATRYPMLPLARLVRRLRPAVVLATLRMGLTAGLA
                     RPLFPRATRLIVRPANHLSQNHGELIRGAPLKHRISYALSRLALTRADHLICQGEDLA
                     GDLARLGVTTPMTVIGNPIDVDDVARLAAAPATLPGSPALLAVGRLSRQKGFDLLVAA
                     FARLLATAPAAHLTIAGT"
BASE COUNT          327 a          790 c          793 g          256 t
ORIGIN      
        1 ggccagcacg agcgcgaacg gcaggcccgc cgcgcgcagc gcctgggcga gcggccagcc
       61 gaccgcggtc acggcggcgg cgagcacggc gcccgggacg tgagcggcca ggaagctccc
      121 gggcggcagc tccgagcacc gcatcgcgag caggcagcag tgcaggctga cggtggtgat
      181 cgccagcgcg gtcgacaccg cgaccccggc gatcccccag cgcaccgtga tcagcgcgcc
      241 gccgacgacc acgaccatgt acacgacgta ggcggcggcg accgtgttca ccgcgcccgc
      301 ggcctgggcc aggatgccgc cgagcttgag gttggtgcgg agcaggatcg tgaccgacag
      361 gatgcggaac ggcagcacca tctcgcccca gttcgggccc atcagcagcc ggatcgcctc
      421 cggggcgagc acgatgatcg cggcgctcgc cggcagcagc acgagcgcgt tgacgaacgc
      481 ggtccgccgg aggtggctcg ccagggcgcg gcggtcgtcc tgcagccgcg cgaacgccgg
      541 gaacagcacg ttgccgacga tcgcgtcgaa caccatcgac gggaacttga tcaggtcgta
      601 ggcccgcgag tagtagccga ggtgggtcgc gccgagcacg tgaccgacga cgaagttgtc
      661 gccgtaggtg gcgaccacgt tggtgctctg gatcgccgac tggccggccc cgaagcgcag
      721 cagctcgcgc agccgggcgc ggtcgaccgc gagcgagtag cgcggcgggc tgagtcgcag
      781 gtacagcgcg gtcgcgatca gctcctcgat caggtagccg gcgaccagcg cccacgggcc
      841 gaggccggcg agcgcgcacg cgatgctgac gaccgagccg gtgaggaacg ccgcggcgtc
      901 gacgatcgcg atccgccgga agttcatcgc gcgctggcac agcatgcgcg acgtggtcgc
      961 caggctgcgc agcccgaaga acaccacggc gagcacgcgg atcacgtcga ccgactcggg
     1021 gatgccgtag aacctcccga tcagcggcgc ggcgagccac agcagcgccg tcagcgcgac
     1081 gccgaggtag accgagaccg cgaacgcggt ggtcaggtgg ccgggctcga gctgcttgcg
     1141 ctggaccagc gccaggccga tgccgacgtc gcggatgccg tagacgacca cgttgaccga
     1201 caccgcggcg acgacgatcc cgaagtccgt cgaggtcagc gcgcggccga gcacggtgac
     1261 cacgacgagc cgcatgccgt tggtcgcgac cgcgccggcg aagctccacg agatcccgcg
     1321 ccgggtctgg cgaccgacct gctccgagga cgacacgctc cgacgagcgt agcgggcctt
     1381 cgccacccgc tcaagcgatc cgcgccggcg cgtgaacgca gccgccgccg gcggtcacca
     1441 atcggtgagg ccgtctgccg tgaccgagcg ccggatcagg atcctgttcg cgattcccgc
     1501 gctcgacaag ggcggccccg atcgcgtgtt cttcgagctg ctgcgcgcgc tcgaccgcgc
     1561 acggttctcg ccggtgctgg tcaccagcga gccgaccggt cactacctgt cgcgcttgcc
     1621 cggcgacgtc gagctccatc acctcgggcg cgaggtcggg atcgcgacgc gctatcccat
     1681 gctgccgctc gcgcgcctgg tgcgccggct gcgtccggcg gtcgtgctgg cgacgctgcg
     1741 gatggggctg accgcggggc tggcgcggcc gctgttcccg cgcgcgacgc gcctgatcgt
     1801 gcggccggcc aaccacctgt cgcagaacca cggcgagctg atccgcggcg cgccgctcaa
     1861 gcatcgcatc agctatgcgc tcagccggct cgccctgacc cgcgccgatc acctgatctg
     1921 ccagggcgag gacctcgccg gcgacctggc gcggctcggg gtcacgacgc cgatgacggt
     1981 gatcggcaac ccgatcgacg tcgacgatgt cgcgcgcctg gcggccgccc cggcgacgct
     2041 gccgggcagc ccggcgctgc tcgcggtcgg ccggctcagc cggcagaagg gcttcgacct
     2101 gctggtggcg gcgtttgcgc ggctgctggc caccgcgccc gccgcgcacc tgacgatcgc
     2161 cggtac
//
LOCUS       DASVOR010000237         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_528200,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000237 DASVOR010000000
VERSION     DASVOR010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528200"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VF516_05180"
     CDS             complement(<1..202)
                     /locus_tag="VF516_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107098.1"
                     /translation="MAEPIARQAFVAGRAPGGLARALADRDAGIARIRTVDDHVVQRL
                     LPGDAERHADVALSIGIDQQAVV"
     gene            261..1274
                     /locus_tag="VF516_05185"
     CDS             261..1274
                     /locus_tag="VF516_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8107099.1"
                     /translation="MEKTCSSCGQVFRPAFVFQIAVTADGEPNHFCSLECRTARLGAE
                     GYRGKRARRIAILNQKGGTGKTTTAVNLAAGLAERENDCLLIDTDAQGNVGVSLGVAG
                     EQSLYHVIVDGADPCDASVPVRQHLDVITSDSTLAAAEIWLARQHPDQRWKIMTKRLN
                     LMQVSRRYDYVIIDCGPSLNLLNQNALSYADEVIIPVTCDYLALVGVKQVLRTIKDVE
                     RHLGHAVRVSAVLPTFYDGRTRLAREVLETLQGHFKHKCLEPIRHNTRLAEAPSHKKT
                     IFEYAPASHGATDYSRVCDWVITTTSQVEATLFSGPGPGPGPSPAPRPRPDIARPEVR
                     EVP"
     gene            1271..1591
                     /locus_tag="VF516_05190"
     CDS             1271..1591
                     /locus_tag="VF516_05190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107100.1"
                     /translation="MKDDKLMAHNPLSDADAILSEEYYGRRARTDDAAPGSGARRRAA
                     SGQVKPTHYKVICISMYTRDIEELEAKVAELKRRGWTKASKSQLIRLALAQVDLDKLA
                     TPRQ"
     gene            complement(1615..>2861)
                     /locus_tag="VF516_05195"
     CDS             complement(1615..>2861)
                     /locus_tag="VF516_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107101.1"
                     /translation="NTRRRPFVDGRGTTRGFGGEAGPDDRIGADATGDGDDAPRPSGK
                     VARSDDGDRPARKKSRGDDGDDRPARKSRGDDDDARPGKVGDDGDDAKEASRPTAHVA
                     KTTDIYNEPNAGSDASFTAGPRTALYVVEEKGKWTFVENDEGDAGYVLTSKLDIDAPA
                     GGPRALRIELAGGLGLAVIGQSVSTAGGQAGVPDNYSAGSSSIGLALGGEALYPYQKR
                     YWLGGELGYAYTTTLFGGIENKGATVGFSFHDLDLRALGGYDLQDRYGMVVFGRLGYH
                     YESFQLSNVSDFNQNTAKLPNQIIQGPTLGAGLAISRLTSSLALQTSLDVMLIGASLQ
                     QTQNLEDGTGPGAKAVYVGAALSYHWKPKFDLRAAVDVSHTSISFDGPAPTTSTRGHM
                     STTPSSGSDTNYALSLGVVYPL"
BASE COUNT          417 a         1000 c          995 g          449 t
ORIGIN      
        1 gaacgactgc ctgctgatcg ataccgatgc tcagggcaac gtcggcgtgt cgctcggcgt
       61 cgccggggag cagtcgctgt accacgtgat cgtcgacggt gcggatccgt gcgatgccag
      121 cgtcccggtc cgccagcgcg cgcgcgagcc cccccggcgc gcggccggcc acgaacgcct
      181 gacgtgcgat cggctccgcc acgccaccac tatagcgccg aaaacttgcc acggctccga
      241 cgatgccgct tcgataaggc atggagaaga cgtgcagctc gtgcggccag gtgttccggc
      301 cggcgttcgt attccagatc gcggtgactg cggacggcga gcctaaccac ttttgctcgc
      361 tcgagtgcag aactgcacgt ctcggtgccg agggctaccg tggcaagcgc gcacgccgga
      421 tcgccattct caaccagaag ggcggcacag gcaagaccac gacggcggtc aatctcgccg
      481 cgggcctggc cgagcgtgag aacgactgcc tgctgatcga taccgatgct cagggcaacg
      541 tcggcgtgtc gctcggcgtc gccggggagc agtcgctgta ccacgtgatc gtcgacggtg
      601 cggatccgtg cgatgccagc gtcccggtcc gccagcacct cgacgtgatc acgtcggact
      661 cgacgctggc cgccgccgag atctggctgg cgcggcagca tccggaccag cgctggaaga
      721 tcatgaccaa gcgcctgaat ctcatgcagg tatcgcggcg ctacgattac gtgatcatcg
      781 attgcggccc gtcgctcaac ctgctcaatc agaatgcgct gtcctacgcc gacgaagtga
      841 tcattccggt gacatgcgac tacctggcgc tggtcggcgt gaagcaggtc ttgcgcacga
      901 tcaaggacgt cgagcgccac ctcggccacg cggtgcgggt gtccgccgtg ctcccgacgt
      961 tctacgacgg gcggacccgg ctcgcgcgcg aggtgctgga gaccttgcag ggccacttca
     1021 agcacaagtg cctggagccg atccgccaca acacgcggct cgccgaggcg ccgagccaca
     1081 agaagacgat cttcgagtac gcgccggcgt cgcacggcgc caccgactac agccgggtct
     1141 gcgactgggt gatcaccacc acatcgcagg tcgaggccac gctgttctcc ggccccggcc
     1201 ccggccccgg cccgagcccg gcgccccggc cgcggcccga catcgccagg cccgaggtcc
     1261 gcgaggttcc atgaaggacg acaagctgat ggctcacaat ccgctgagcg acgccgacgc
     1321 gatcctgtcc gaggagtact acggccggcg ggcgcgcacc gacgacgccg cgccgggctc
     1381 cggagcgcgg cgacgcgccg cgtccggcca ggtcaagccg acccactaca aggtgatctg
     1441 catctcgatg tacacccgcg acatcgagga gctggaggcc aaggtcgccg agctcaagcg
     1501 ccgcggctgg accaaggcga gcaagtcgca gctgatccgg ctcgcgctgg cgcaggtcga
     1561 tctcgacaag ctggcgacgc cgcggcagta ggccacgggt cgcggggccg ctcgctacag
     1621 cgggtacacc acgccgagcg agagggcgta gttggtgtcc gatcccgagc tcggcgtggt
     1681 gctcatgtgc ccgcgcgtcg acgtcgtcgg cgccgggccg tcgaagctga tcgacgtgtg
     1741 gctcacgtcg accgcggcgc gcagatcgaa cttcggcttc cagtggtagc tgagcgcggc
     1801 gccgacgtac accgccttgg cgcccggacc ggtcccgtcc tccaggttct gggtctgctg
     1861 gaggctggcg ccgatcagca tgacgtccag gctggtctgg agcgccaggc tgctggtcag
     1921 ccgagagatc gccaggccgg cgccgagcgt cgggccctgg atgatctggt tgggcagctt
     1981 ggcggtgttc tggttgaagt cgctgacgtt ggagagctgg aacgactcgt agtggtagcc
     2041 gagccgcccg aacacgacca tgccgtacct gtcctggagg tcgtagccac cgagcgcgcg
     2101 gaggtcgagg tcgtggaacg agaacccgac ggtcgcgccc ttgttctcga tcccgccgaa
     2161 cagcgtcgtg gtgtaggcgt agccgagctc gccgccgagc cagtagcgct tctggtacgg
     2221 gtacagcgcc tcgccgccga gcgcgagccc gatcgacgag ctgcccgcgc tgtagttgtc
     2281 cggcaccccc gcctggccgc cggcggtgct caccgactgg ccgatcaccg cgaggcccag
     2341 tccgccggcg agctcgatcc ggagcgcgcg cgggccgccg gccggtgcgt cgatgtcgag
     2401 cttggaggtc agcacgtagc cggcgtcgcc ctcgtcgttc tcgacgaacg tccacttgcc
     2461 cttctcctcg acgacgtaca gcgcggtcct gggcccggcg gtgaacgagg cgtcgctgcc
     2521 ggcgttcggc tcgttgtaga tgtcggtggt cttggcgacg tgggcggtcg gtcgcgacgc
     2581 ttccttggcg tcgtcgccgt cgtcgccgac cttgcccggc ctggcgtcgt cgtcgtcgcc
     2641 ccgggacttt ctggccggcc ggtcgtcgcc gtcgtcgccg cgggacttct tgcgcgccgg
     2701 ccggtcgccg tcgtcgctcc gcgcgacctt gccgctcggc ctgggcgcgt cgtcgccgtc
     2761 gccggtcgcg tcggcgccga tccggtcgtc cgggccggcc tcgccgccga acccgcgcgt
     2821 ggtgcctcga ccatcgacga acggtcgccg gcgggtgttg c
//
LOCUS       DASVOR010000238         5094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_469829,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000238 DASVOR010000000
VERSION     DASVOR010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5094
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_469829"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VF516_05200"
     CDS             complement(<1..1109)
                     /locus_tag="VF516_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8107102.1"
                     /translation="MNRRTTAQAVRRPVATATPVEFKLNGKTVIGRSDEPIIETAQNH
                     GIAIPRLCYREGMRPDGNCRACVVEIKGERVLAPSCCRFPTSGMEVTTDSPRALASQK
                     IVIELLLADMPEQRYTPSNEVDAYAKQFQIGRSRFPARGQPKADLSHPAIAVHLDACI
                     QCTRCVRACREEQVNDVIGYAFRGEHSKIVFDLDDPMGASTCVACGECVQVCPTGALM
                     PARDVGLAKADRTVDSVCPYCGVGCLLTYHIRDNKILHVQGRDGPANQSRLCVKGRYG
                     FDYVHHKQRLTTPLVRKPGVPKTKDFTVHPEHWHEAFREATWEEALELAASGLRNIRD
                     RHGKKALAGFGSAKCTNEEAYLFQKLVRTGFGSNNV"
     gene            complement(1114..3045)
                     /locus_tag="VF516_05205"
     CDS             complement(1114..3045)
                     /locus_tag="VF516_05205"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEX8107103.1"
                     /translation="MTHRATTGPRPAIAVAGRIDRRPCRDGVRACYPSVPCPGRRARC
                     GSVGARPEMSDRPRPNSPIRVGKLRSTPKGRTVDSQALQEVQQLLRDAPRRRDLLIEH
                     LHKIQDRFGCLADRHLVALAHEMKLAMTEVYEVATFYHHFDVVKDGEPAPPPVTVRVC
                     DSLACELAGAQELLERLPSLLGKGVRVVPAPCIGRCEQAPAATVGQHPVPSADLIAIE
                     ALVKRGDAKHPAARDRAQFAPDSLIGAGRSVGVARDVNPDYVGYDAYRQAGGYRLIAE
                     CLAGKHDPEAIIQKMEHSGLRGLGGAGFPSGRKWRIVRGEPQPKVVAVNIDEGEPGTF
                     KDRVYLERDPHRFLEGMLIGAWAVQAGACYVYLRDEYHGCRAILENELARLTADPPCG
                     LPPIDLRRGAGAYICGEESAMIESIEGKRGMPRLRPPYVAQVGLFGHPTLEHNMETLY
                     WVRDILERGPEWFAGFGRNGRKGLRSFSVSGRVKNPGMHLAPAGITVQQLIDEYCGGM
                     LDGHVFYAYLPGGASGGILPASLGDIPLDFDTLNKYGCFIGSAAVVVLSDHDRASDAA
                     RNVLRFFRDESCGQCTPCRVGTAKALALMERPLWDEPLLEELSQAMMDASICGLGQAA
                     PNPLLCVMKYFRHELQQGA"
     gene            complement(3077..4708)
                     /locus_tag="VF516_05210"
     CDS             complement(3077..4708)
                     /locus_tag="VF516_05210"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEX8107104.1"
                     /translation="MDAFDPSPLNLLDRERIVARPGFNRWLVPPAALAIHLCIGMAYG
                     FSVFWLPLSRALGISKSVVCKDMSLIDALTTTSCDWRVSDVTWMYTLFFVFLGSSAAV
                     FGGWLERAGPRKAGVAAAVCWCVGLLISAVGVITHQLWLMWLGSGVIGGIGLGLGYIS
                     PVSTLIKWFPDRRGMATGMAIMGFGGGAMIGAPLADKLMNTFRGADSVGVWQTFAVLA
                     AIHFVFMISGAFGYRVPPTGWVPDGWTPPPTKAMITAGHVHLKDAHKTKQFWLIWWVL
                     CLNVSAGIGVIAMASPMLQEIFGGKLIGAASLGFTQLDDSQKAKIATIAAAFTGLLSL
                     FNIAGRFFWASISDKIGRKLTYATFFVLGITLYALAPSAARAGIVVLFVVMMCVILSM
                     YGGGFSTVPAYLADIFGTQFVGAIHGRILTAWSAAGIFGPVLINKIRDAQIDAGVPRD
                     RVYDRTMYILAGFLVVGFIANFLIKPLAAKWFMSKAEVAELQSKSKNAAAPSGSHGIG
                     LGSFTPQVALAWLAVGIPLAWGVWVTIDKARPLFS"
BASE COUNT          854 a         1753 c         1694 g          793 t
ORIGIN      
        1 acattgttcg agccaaaacc ggttcgcacg agcttctgga acagatacgc ttcctcgttg
       61 gtgcacttgg cggacccgaa gccggcgagc gccttcttgc cgtggcggtc gcggatgttg
      121 cgcaacccgg aagccgcaag ctcgagcgcc tcttcccagg tcgcctcgcg aaatgcttcg
      181 tgccagtgct ccggatgaac ggtgaaatcc ttggtcttcg gcacgccggg cttgcgcacc
      241 agcggcgtgg tgagccgctg cttgtgatgg acgtagtcga acccgtagcg ccccttcacg
      301 cacagccggc tctggttcgc cggaccgtcc cggccctgca cgtgcaggat cttgttgtcc
      361 cggatgtgat aggtcagcag acagcccacg ccgcagtacg ggcataccga gtcgacggtc
      421 cggtccgcct tcgcgagccc gacgtcacgc gcgggcatga gcgcgccggt cgggcacacc
      481 tgcacgcatt cgccgcacgc gacgcaggtg gacgcgccca tcgggtcgtc gagatcgaac
      541 acgatcttcg agtgctcgcc gcggaacgcg tacccgatga catcgttcac ctgctcctcg
      601 cggcacgcgc gcacgcagcg cgtgcactgg atgcacgcgt cgagatggac cgcgatcgcc
      661 gggtgcgaga gatcggcctt gggctggccg cgtgccggga agcgcgaccg accgatctgg
      721 aactgcttcg cgtaggcgtc gacctcgttg ctgggcgtgt aacgctgctc cggcatgtcc
      781 gcgagcagca gctcgatcac gatcttctgc gatgcgagcg cgcgcgggct gtcggtcgtc
      841 acctccatgc cgctcgtcgg gaaccggcag caggatggcg cgagcacgcg ctcgcccttg
      901 atctcgacga cgcaggcgcg gcagttgccg tcgggccgca ttccctcgcg gtagcacagt
      961 cgcggaatcg cgatgccgtg gttttgcgcc gtctcgatga tcggctcgtc cgagcggccg
     1021 atgaccgtct tgccgttgag cttgaactcg accggcgtcg cggtcgcgac cggcctcctg
     1081 accgcttgag cggtggtccg tcgattcatg ccatcacgct ccttgctgca actcgtgcct
     1141 gaagtacttc atcacgcaca gcagcgggtt cggcgccgcc tgccccaggc cgcagatcga
     1201 tgcgtccatc atcgcctgcg acaactcctc cagcagcggc tcgtcccaga gcggccgttc
     1261 catgagcgcc aatgccttgg ccgtgccgac ccggcacggc gtgcactgcc cacaggattc
     1321 gtcgcggaag aaccgcaaca cgttccgtgc cgcgtcgctc gcgcggtcgt ggtccgagag
     1381 aacgacgacc gccgccgagc cgatgaaaca gccgtacttg ttcagcgtgt cgaagtcgag
     1441 cgggatgtcg cccagcgacg ccggcaggat gccgcccgac gcgccgccgg gcagataggc
     1501 gtaaaacacg tgtccatcca gcattccgcc gcagtactcg tcgatcaact gctgcaccgt
     1561 gatgccggcg ggcgcgaggt gcatgcccgg gttcttgacg cggcctgaga ccgagaacga
     1621 gcgcagaccc ttgcgtccgt tgcgcccgaa acccgcgaac cactcgggcc cgcgctcgag
     1681 gatgtcgcgc acccagtaga gcgtctccat gttgtgctcg agcgtgggat ggccgaacag
     1741 gcccacctgc gccacgtacg gtgggcgcag ccgcggcatg ccgcgcttgc cctcgatcga
     1801 ctcgatcatg gccgactctt cgccgcagat ataggctccc gccccgcgac gcagatcgat
     1861 cggcggcaac ccacagggtg gatccgccgt caaccgcgcg agctcgttct ccaggatcgc
     1921 gcggcagccg tgatactcgt cgcggagata cacgtagcat gcgccggcct ggaccgccca
     1981 cgcgccgatc agcatgccct cgaggaagcg gtgcggatcg cgctcgagat agacccggtc
     2041 cttgaacgtg ccgggctcgc cttcgtcgat gttgacggcg accaccttgg gctgcggctc
     2101 gccgcggacg atgcgccact tgcggccgct ggggaacccc gcgcccccga gcccgcgcaa
     2161 gccggagtgc tccatcttct ggatgatcgc ctccgggtcg tgcttgccgg cgaggcactc
     2221 ggcgatcagg cgatagccgc ccgcctggcg gtaggcgtcg tagccgacgt agtccggatt
     2281 cacgtcgcgc gcgacgccca ccgatctgcc ggctccgatc agcgaatcgg gtgcgaactg
     2341 cgcgcgatct cgcgcggcgg gatgcttcgc gtcaccgcgc ttcaccagcg cttcgatcgc
     2401 gatcagatct gcggagggaa ccggatgctg ccccacggtc gcggcgggtg cctgctcaca
     2461 acggccgatg cacggcgcgg gcacgacgcg aacacccttg ccgagcagcg aggggagccg
     2521 ctcgagcagc tcctgggcac cggcaagctc gcacgcgagc gagtcgcaca cccgcaccgt
     2581 gaccggggga ggtgccggct ctccatcctt cacgacgtcg aagtgatgat agaaggtcgc
     2641 gacctcgtac acctcggtca tggcgagttt catttcgtgc gcgagcgcca ccagatgacg
     2701 gtcggcgagg caaccaaacc gatcctggat cttgtgcaaa tgctcgatca gcagatctcg
     2761 ccggcgagga gcatcgcgga ggagttgctg gacctcctgc agcgcctgcg aatcgaccgt
     2821 ccggcccttg ggggtggacc gcagttttcc aacgcggatc gggctgttag ggcggggtcg
     2881 gtcgctcatc tcgggtcttg ctcccacgct gccgcaccgc gcgcggcggc cgggacaggg
     2941 gacgctaggg tagcacgcac ggacgccgtc ccgacatgga cggcgatcga tcctgcctgc
     3001 cacggcgatc gccggccgcg gtccggtcgt ggctcgatgc gtcatccatg ggatcccatg
     3061 gtttttgtcc gggcggtcag ctgaacagcg ggcgcgcctt gtcgatcgtg acccacacgc
     3121 cccacgccag cgggatgccg accgcgagcc aggcgagggc gacctgcggc gtgaacgagc
     3181 ccagcccgat cccgtgcgag cccgacggcg cggcggcgtt cttgctcttg ctctggagct
     3241 ccgcgacctc ggccttggac atgaaccact tcgccgccag cggcttgatc aggaagttgg
     3301 cgatgaagcc gaccacgagg aacccggcga ggatgtacat cgtgcggtcg tagacccggt
     3361 cgcgcgggac gccggcgtcg atctgcgcgt cgcggatctt gttgatcagc accggaccga
     3421 agatgcccgc cgccgaccac gcggtgagga tccggccgtg gatcgcgccg acgaactggg
     3481 tgccgaagat gtcggcgagg taggccggga ccgtggagaa gccgccgccg tacatcgaca
     3541 ggatgacgca catcatcacg acgaacagca cgacgatgcc ggcgcgcgcg gccgatgggg
     3601 ccagcgcgta cagcgtgatg cccagcacga agaacgtggc gtaggtcagc ttgcgcccga
     3661 tcttgtccga gatcgacgcc cagaagaacc ggccggcgat gttgaacagc gacaagaggc
     3721 cggtgaacgc ggcggcgatg gtggcgatct tcgccttctg gctgtcatcg agctgggtga
     3781 agcccagact cgcggcaccg atcagcttgc cgccgaagat ctcctgcagc atcggcgacg
     3841 ccatggcgat cacgccgatc ccggcgctga cgttgaggca cagcacccac cagatcagcc
     3901 agaactgctt ggtcttgtgc gcatccttca ggtggacgtg gccggcggtg atcatcgcct
     3961 tggtcggcgg cggcgtccat ccgtcgggca cccagccggt gggcggcacg cggtagccga
     4021 acgcgccgct gatcatgaac acgaagtgga tcgcggcgag caccgcgaac gtctgccaca
     4081 cgccgacgct gtcagcgccg cggaacgtat tcatcagctt gtcggccagc ggtgcgccga
     4141 tcatcgcacc acccccgaag cccatgatcg ccatgccggt ggccatgccg cggcgatccg
     4201 ggaaccactt gatcagcgtc gataccggcg agatgtagcc caggccgagg ccgatgcctc
     4261 cgatcacgcc cgagccgagc cacatcagcc acagctggtg ggtgatgacg cccaccgcgg
     4321 agatcagcag gccgacgcac cagcacaccg ccgccgccac gccggccttg cgcggcccgg
     4381 cgcgctccag ccacccgccg aacaccgcgg ccgacgagcc gaggaacacg aagaacagcg
     4441 tgtacatcca ggtgacgtcg ctgacccgcc agtcgcagct ggtcgtggtc agcgcgtcga
     4501 tcagcgacat gtccttgcac accaccgact tggagatgcc aagggcgcgc gacagcggca
     4561 gccagaacac gctgaagccg tacgccatgc cgatgcacag atggatcgcg agcgcggcgg
     4621 gcggcaccag ccagcggttg aacccgggcc gggcgacaat gcgttcacga tcgaggaggt
     4681 tgagcggact cggatcgaag gcgtccatgc aagaccttcc tgcccggggg atgcgtcccg
     4741 catcctccgc gcgtcccgca tcctaacaag cgcgtcgcgc gagaaacaag cgacgaagca
     4801 tgggaaccca tgtttctcga ttgtgaagca tgggttccca tgctttgcgg accgagccct
     4861 ccctacccct gactcgcgcg gacgctccgg gtcagggacg cgcctggacc gtgattagcg
     4921 gtccatcacc gcgcgatcgg aactgcggcg ctcacggcac acggggccgc gctactcgat
     4981 gtagcgcatg ccgagctcgt gcatcaggaa cgcgtagctg tcggcggcct cgtcgatcac
     5041 ggcgccgagc gggctgccga tgccgtggcc gacgtcgtcg ctggcgcgca gcag
//
LOCUS       DASVOR010000239         3325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265162,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000239 DASVOR010000000
VERSION     DASVOR010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3325
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265162"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1453
                     /gene="ftsH"
                     /locus_tag="VF516_05215"
     CDS             <1..1453
                     /gene="ftsH"
                     /locus_tag="VF516_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEX8107105.1"
                     /translation="AKDEVEEIIAFLKDPKKFTRLGGRIPKGVLMMGPPGTGKTLLAR
                     AIAGEAGVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRG
                     AGLGGGHDEREQTLNQLLVEMDGFESNDGVIIIAATNRPDVLDPALLRPGRFDRRIVV
                     PRPDLRGRMGILQVHTRKVPLGGVDLEVLARGTPGFSGADLEFLVNEAALIAARRDKD
                     KVEMEDFEEAKDKVLMGAERRSMIISEKEKRTTAVHEAGHALVARFIDTLGGDSVDPV
                     HKVTIIPRGRALGVTQQLPHEDRLNMTREFALNQIAILMGGRVAEEIVFGQKTTGAGN
                     DIERATELARSMVTEWGMSDEFGPLNFSAGKQEVFLGRDFSSSDRHSEDTSQRIDAEI
                     RRIVTDQYTRAKDILTLHRKELEQIAEALLEYETLDGDDIDTLLRGERLDRPVSAAAR
                     KRADAGAEKDETERKRPSILPPLGKKDPSPEPA"
     gene            1517..2401
                     /locus_tag="VF516_05220"
     CDS             1517..2401
                     /locus_tag="VF516_05220"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEX8107106.1"
                     /translation="MAEVPDPTARVLVMGVVNVTPDSFSDGGRFFAADAAVAHGLQLV
                     REGADIVDVGGEATHPRATPITVDEELRRALPVVERLAAAGAVVSIDTTKAVVARAAV
                     AAGARIINDVSGGLFDPDMAHAIGEATYIAGHLRGRSLSEVFSAEARGCSAWGCSAWG
                     CSAWGCSAWRDAPLDWRTVADELAERLAALPVAAQRRAWVDPGLGFGKGPDPEVNLAL
                     LRHSGELGARLGRPVVVGPSRKRFVRRAFGVGDDDLPVLDAASVTASLAGIAGGANVV
                     RVHNVALLRAAIALYTKD"
     gene            2456..>3325
                     /gene="cdaA"
                     /locus_tag="VF516_05225"
     CDS             2456..>3325
                     /gene="cdaA"
                     /locus_tag="VF516_05225"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEX8107107.1"
                     /translation="MTASLREFLDGLTATNVATTVVDVALVYYLIYRLLLTIRGTRAA
                     QMVVGIVLVGAAFFVAERLDLSTVSWLLDNFISYFIILIIVVFQQDIRRALGRIGQNV
                     MPLGRRQETTHTVDEVVAATAQLARARMGAIIVFERDAALEEFVDDATRIDAVLSRQL
                     LVSLFVPARDNELHDGAVVIGKSHRIELARALLPLSRATDLGPEFGTRHRAALGITED
                     TDAVSVVVSEERGEISLCFKGSIARDLEPVLLRRALVGLFAGGRATEDMAEEAEAAAE
                     VGKALATLGEPRTE"
BASE COUNT          486 a         1138 c         1232 g          469 t
ORIGIN      
        1 agccaaggac gaggtcgagg agatcatcgc cttcctcaag gatcccaaga agttcacccg
       61 gctcggcggc cggatcccca agggcgtcct gatgatgggc ccgccgggca ccggcaagac
      121 gctgctcgcg cgcgcgatcg cgggcgaggc cggggtgccg ttcttctcga tcagcggctc
      181 ggacttcgtc gagatgttcg tcggcgtcgg cgccagccgc gtccgcgacc tgttcgagca
      241 gggcaagaag aacgcgccct gcatcatctt catcgacgag atcgacgcgg tcggccgcca
      301 ccgcggcgcc ggcctgggcg gcggtcacga cgagcgcgag cagacgctca accagctgct
      361 ggtcgagatg gacggcttcg agtccaacga cggcgtgatc atcatcgccg cgaccaaccg
      421 ccccgacgtg ctcgatccgg ccctgctgcg gccgggccgc ttcgatcgcc ggatcgtcgt
      481 gccgcgcccc gatctgcggg gccggatggg catcctgcag gtccacaccc gcaaggtccc
      541 gctcggcggg gtcgacctcg aggtcctcgc ccgcggcacg ccggggttct ccggcgccga
      601 cctggagttc ctggtcaacg aggcggcgct gatcgccgcg cgtcgcgaca aggacaaggt
      661 cgagatggag gacttcgagg aggccaagga caaggtcctg atgggggccg agcggcgctc
      721 gatgatcatc tccgagaagg agaagcgcac gaccgcggtc cacgaggccg gccacgcgct
      781 ggtcgcccgg ttcatcgaca cgctgggcgg cgactcggtc gacccggtcc acaaggtcac
      841 gatcatcccg cgcggtcgcg cgctcggcgt cacccagcag ctgccgcacg aggaccggct
      901 caacatgacc cgcgagttcg cgctcaacca gatcgcgatc ctgatgggcg ggcgggtcgc
      961 cgaggagatc gtgttcggcc agaagaccac cggcgccggc aacgacatcg agcgggcgac
     1021 cgagctggcg cgctcgatgg tcaccgagtg gggcatgagc gacgagttcg gcccgctgaa
     1081 cttctcggcc ggcaagcagg aggtgttcct gggccgcgac ttctcgtcga gcgaccgcca
     1141 cagcgaggac acgtcgcagc ggatcgacgc cgagatccgt cggatcgtca ccgatcagta
     1201 cacccgcgcg aaggacatcc tgaccctcca ccgcaaggag ctcgagcaga tcgccgaggc
     1261 gctgctcgag tacgagacgc tggacggcga cgacatcgat acgctgttgc gcggcgagcg
     1321 cctggaccgc ccggtgtcgg cggcggcgcg caagcgcgcg gatgcgggtg cggaaaagga
     1381 tgagaccgag cgcaagcgcc cgtcgatcct gccgccgctg ggcaagaaag acccgtcgcc
     1441 cgagccggcg tgacccggca aggtccggca acacggctac tcgcggcccg gccgcaccgc
     1501 tgacgtgagg tccgcgatgg cggaggtccc tgacccgacg gcgcgcgtgc tggtgatggg
     1561 cgtcgtcaac gtcacgccgg actcgttctc cgacggcggg cggttcttcg ccgccgatgc
     1621 ggcggttgcg cacgggctgc aactggtgcg cgagggcgcg gacatcgtgg atgtcggggg
     1681 cgaggcgacc cacccgcgtg cgacgccgat cacagtggac gaggagctgc ggcgcgccct
     1741 gccggtggtg gagaggctcg cggcggccgg cgccgtggtc tcgatcgaca ccaccaaggc
     1801 ggtcgtggcg cgcgccgcgg tcgcggccgg cgcccggatc atcaacgatg tgtccggcgg
     1861 tctgttcgat ccggacatgg cacacgcgat cggcgaggcc acctacatcg ccggccacct
     1921 gcgcggccgc tcgctatccg aggtgttctc ggccgaggcg cgggggtgca gcgcgtgggg
     1981 gtgcagcgcg tgggggtgca gcgcgtgggg gtgcagcgcg tggagggatg caccgctcga
     2041 ctggcgcacc gtcgccgacg agctcgccga gcggctcgcg gcgctgccgg tcgccgcaca
     2101 gcgtcgagcg tgggtcgatc cggggctggg gttcggcaag gggccggacc cggaggtcaa
     2161 cctcgccctg ctgcgccaca gcggggagct cggcgcccgg ctcggccggc ccgtggtggt
     2221 ggggccgagc cgcaagcgat tcgtccgccg tgcgttcggt gtgggtgatg acgatctgcc
     2281 ggtgctggac gccgcctcgg tcaccgcgag tctcgctggc atcgcgggcg gcgcaaatgt
     2341 ggtgcgggtc cacaacgtcg cattgctcag ggccgcgatc gcgttgtaca ccaaagacta
     2401 gggcgcgccg cgcggcgacc gcgcggggcc gcacgcccga ccgaaacgtg ctcccgtgac
     2461 cgcgtcgctc cgtgaatttc tcgacggact caccgccacc aacgtcgcga cgacggtggt
     2521 ggacgtcgcg ctggtctatt acctgatcta tcgcctgctg ctgacgatcc gcggcacgcg
     2581 cgcggcccag atggtggtcg gcatcgtgct ggtcggcgcg gcgttcttcg tcgccgagcg
     2641 gctcgatctg tcgacggtgt cgtggctgct cgacaacttc atctcgtact tcatcatcct
     2701 gatcatcgtc gtgttccagc aggacatccg gcgcgcgctc ggccggatcg gccagaacgt
     2761 gatgccgctc ggccgccgcc aggagaccac ccacaccgtc gacgaggtcg tcgccgccac
     2821 cgcgcagctc gcgcgcgccc ggatgggggc gatcatcgtg ttcgagcgcg acgcggcgct
     2881 cgaggagttc gtcgacgacg cgacccggat cgacgcggtg ctgtcgcgcc agctgctggt
     2941 gtcgctgttc gtgccggcgc gcgacaacga gctgcacgac ggcgccgtgg tgatcggcaa
     3001 gagccaccgg atcgagctgg cgcgcgcgct gttgccgctg tcgcgcgcca ccgacctggg
     3061 ccccgagttc ggcacccgcc accgcgcggc gctcggcatc accgaggaca ccgacgcggt
     3121 gagtgtggtg gtgtcggagg agcgcggcga gatctcgctg tgcttcaagg gctcgatcgc
     3181 gcgcgacctc gagccggtgc tgctgcggcg cgcgctggtc ggcctgttcg cgggcggtcg
     3241 cgccaccgag gacatggccg aggaggccga ggccgccgcc gaggtcggca aggcgctggc
     3301 cacgctgggc gagccgcgaa cggag
//
LOCUS       DASVOR010000240         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_557580,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000240 DASVOR010000000
VERSION     DASVOR010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557580"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VF516_05230"
     CDS             complement(<1..331)
                     /locus_tag="VF516_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107108.1"
                     /translation="MLADRYRLVRCLGRGASCEVHEAVDLALGSTVALKRFRAPSLDA
                     RTQIKAEFRALADLHAPGLVRFFDLTVDGDAAFFTMELVRGVRLDEYARGAGGDALRG
                     VLGRVAAA"
     gene            complement(648..914)
                     /locus_tag="VF516_05235"
     CDS             complement(648..914)
                     /locus_tag="VF516_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107109.1"
                     /translation="MQPAIAPSPAAGWLWLDGTAVTGGWGGVEPNDGDGRENKSEQFA
                     RIEKSKPYLQDFTGSGNDSALCECDGKPIAPAAAAAIAANRPPS"
     gene            complement(1034..1543)
                     /locus_tag="VF516_05240"
     CDS             complement(1034..1543)
                     /locus_tag="VF516_05240"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX8107110.1"
                     /translation="MNLYDATVPVFTKLLSNVEKWIDKAAAHADAKKFDVDVLASARL
                     APDQYPFVRQIQAACDQAKYTVAKLTGKEPPSHPDTEKTMAEIRQRIRTVVAYLGSFK
                     REEFQGAEERACSHSWMGGKSLRGGDYLDHFALPNFHFHLTTAYDILRHNGVDVGKMD
                     YIVDLPFRS"
BASE COUNT          249 a          558 c          570 g          265 t
ORIGIN      
        1 cggcggccgc gacccggccg agcacgccgc ggagcgcatc gccgccggcg ccgcgcgcgt
       61 actcgtccag gcgcacgccg cgcaccagct ccatcgtgaa gaacgccgcg tcgccgtcga
      121 ccgtgaggtc gaagaaccgg accagcccgg gcgcgtggag gtcggcgagc gcgcggaact
      181 ccgccttgat ctgggtccgc gcgtcgagcg agggcgcgcg gaaccgcttg agcgccacgg
      241 tcgatccgag cgcgaggtcg accgcctcgt ggacctcgca gctcgccccg cgtcccaggc
      301 accgcacgag gcggtaccga tccgcgagca cttccggatc gctcggcgtc gccactcctc
      361 gaagtccaga cacgcgttcg acgatccagt gtagcgatgc ggctcgactg gatgcgcgca
      421 tgacggcggc gcgaggtcac agcttctatc agaagtgctg tctcgctccg gccattcgcg
      481 aatatccggg acaccgggcg tgcgcaaatc gtgcgtgttg tgcgatccaa gctgacccgg
      541 tcagaaggac tcggagggat cgcgcgaggc cgaggaggac cgctgacagc gcgactcccg
      601 cggtcaggag gaccgctgac agcgcgactc cggaccagcc tgcgcggtca gctcggcggc
      661 cggttggcgg cgatcgcggc ggcggcggcg ggcgcgatcg gcttgccatc gcactcgcac
      721 agcgcgctgt cgttgccgct gccggtgaag tcctgcagat acggcttgct cttctcgatc
      781 cgcgcgaact gctcgctctt gttctcgcgg ccgtcgccgt cgttgggctc gaccccgccc
      841 cagccgccgg tcaccgcggt gccgtcgagc cacagccacc cggcggcggg cgagggcgcg
      901 atcgccggct gcaccgcccc gacccagatc gcgttgccgg cgagcgtcgg ggtcgtggtg
      961 acgcatggaa atatcttaca tatgtctgag tgcgaatctt ccttacaaga tccaaccgct
     1021 gggaacaatg ggatcacgat cggaacggta gatccacgat gtaatccatc ttgccaacat
     1081 ctacgccatt gtgtcggagg atgtcgtagg ctgtagtcag atggaagtgg aagttgggca
     1141 gggcgaagtg atcgaggtag tcgccgccgc gcagcgactt gccgcccatc cacgagtggc
     1201 tacaagcgcg ctcctcggcg ccctggaact cctcgcgctt gaagctgccg aggtaggcga
     1261 ccacggtgcg gatccgctgc cggatctcgg ccatcgtctt ctcggtgtcg gggtgcgacg
     1321 gcggctcctt gccggtcagc ttggcgaccg tgtacttcgc ctgatcgcac gcggcctgga
     1381 tctggcggac gaacgggtac tgatcgggcg cgagccgggc gctcgcgagc acgtcgacgt
     1441 cgaacttctt ggcgtcggcg tgcgcggctg ccttgtcgat ccacttctcg acgttggaca
     1501 ggagcttggt gaacaccgga acggttgcgt cgtacaggtt catggccccg acgctaccgc
     1561 aaacctctcg cagccgccgg cgctcccgat cccccgatcg ggtgggtcga gccggggcgc
     1621 ggcggcgcgc cgctcctgcg gg
//
LOCUS       DASVOR010000241         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_90040,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000241 DASVOR010000000
VERSION     DASVOR010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90040"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..716
                     /locus_tag="VF516_05245"
     CDS             30..716
                     /locus_tag="VF516_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107111.1"
                     /translation="MPPPDGSSGGARARARAAFDEATLDRRTFRHVLVGALRYPPKRL
                     TWVLFRGAARARLQVFCQTGTPPSQQGLSLNGKENDEALWGAPVLTEYAGARSSENPL
                     SYRLPATAAVAGDSGCEPLPGVVNLVCRNEHVSVLAAGAALIPGKKLPDDRMSPARWD
                     PPARERVAALSCDATADGDAGQRLRLVLSSSPLVFAPGKDGGPGIEWAHENSDQAAQD
                     GAYRWMPMRP"
     gene            1012..2100
                     /locus_tag="VF516_05250"
     CDS             1012..2100
                     /locus_tag="VF516_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107112.1"
                     /translation="MSAEWAFEAAPTMPAFPSAYPDPLTPNSKLQTCLDRAIAAGPGT
                     SWRTPVTIVSLNPDGSRPVASFKGNEVHFSASLVKVAAMYAAFELRATLRDIAAELGT
                     STTKATLLKDAAAYLDPKILAKAAAIPALKGITKAHALPQYTTAFEVVPVPPSPLVPS
                     PGFTVNFSSTFQDHLEKMIAISVNQSAAQCIHGSGYGYLNGALASAGFFDPATNNGIW
                     LAGDYIGNYPYFRINSVNDGLVAQAVTTRRLAQMYTLLHDGTLVGGISSSEMRDLLAK
                     AVAVPEVFINRASGLNFTVTHTKVGLGPLKPKNGVIDNVYSEASILQHTPSGRKFVAV
                     WQNFVSGSDGFDPIGHVVRDTIDAFLKP"
     gene            2207..>2890
                     /locus_tag="VF516_05255"
     CDS             2207..>2890
                     /locus_tag="VF516_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX8107113.1"
                     /translation="MEPSGGGTRAPWLDARFDLTARGTTVRREILAGATTFVTMAYVL
                     FVNPQILAAAAGPERLGALLTSTALVAAVMTVAMGVVANLPLALAPGMGLNAVVAFQL
                     VGAAHLSFAEAMGVIVAEGVVITLLVVTGLRQAMVRSVPLPLKRAIGIGIGLFLALIG
                     FKNAGFVSAGGGLLTLGDAGRLHGFPVALFAVTLVFTAWLIARGVRGALLIGMAASTL
                     AAVIARAAFG"
BASE COUNT          397 a         1038 c          995 g          460 t
ORIGIN      
        1 cgtccgccag cgtcgctccg gccggatccg tgcctccgcc cgacggaagc tctggaggtg
       61 cgcgcgcgcg cgcgcgcgcc gccttcgacg aggcgacgct ggatcgccgg accttccggc
      121 acgtcctcgt gggagcgctg cgctatcctc cgaagcggct gacatgggtg ctgtttcgcg
      181 gggccgcgcg ggctcgtttg caggtgttct gtcagaccgg caccccccct tcgcaacaag
      241 ggctgtcgct gaacggaaag gagaacgacg aggccctctg gggcgcgccc gtcctcaccg
      301 agtacgccgg ggcgcgcagc agcgaaaacc cactctccta tcgtctccct gcgaccgcgg
      361 cggtggccgg cgactccgga tgtgagccgc tgccgggtgt cgtcaacctg gtatgtcgca
      421 acgagcatgt ctccgtgctg gccgccggcg ccgcgctgat ccccggcaag aagctgcccg
      481 acgaccgcat gtcccccgcg cgctgggatc cgccggcgcg cgagcgcgtg gcggcgctga
      541 gctgcgacgc cacggcggac ggcgacgcgg gccagcgcct ccgtctcgtg ctgtcctcct
      601 ctccgctcgt cttcgccccg ggcaaggacg gagggcccgg gatcgagtgg gcccacgaga
      661 actcggacca ggcggcgcag gacggcgcct atcgctggat gcccatgcgc ccatgatccc
      721 gtcgacgggc ggaatccatc gtacgagcgc cgagagcgcg tgagcggttg acaagccgac
      781 ggcgatatga tcccttgagt gaacatgctt cgtcagctcc cggggcgccg tcgtttgggt
      841 cggtttcacc gggccgacgc agaggccttc ttccaggatc tgctcggatc cccgggccgc
      901 tcgctcgcat cggaggccga tgagagcgcg tggtcgggcc gagagcgccg gatggctgat
      961 ctgttcctcg ccgatctgat gggagagtct gtccccgtcc ggcgaggatc catgagcgcc
     1021 gagtgggcct tcgaggctgc gccgacgatg ccggccttcc cgagcgcata tccggacccg
     1081 ctgacgccga acagcaagct ccagacgtgc ctcgatcgcg ccatcgccgc cgggcctggg
     1141 accagctggc ggacgccggt gacgatcgtg tcactcaacc ccgacggctc gcgccccgtc
     1201 gcgagcttca agggcaacga ggtccatttc tccgcgagcc tggtcaaggt ggcggcgatg
     1261 tacgcggcct tcgagctgcg cgcgacgctg cgggacatcg ccgccgagct gggcacgagc
     1321 acgaccaagg ccacgctgct gaaggacgcg gcggcctacc tcgaccccaa gatcctggcc
     1381 aaggccgcgg cgatcccggc cctcaagggc atcaccaagg cccacgccct gccgcagtac
     1441 acgacggcgt tcgaggtcgt cccggtgccc ccgtctccgc tggtcccgtc tccggggttc
     1501 acggtgaact tctcgagcac cttccaggac cacctcgaga agatgatcgc gatcagcgtc
     1561 aaccagagcg ccgcgcagtg catccacggc tcggggtatg gctacctcaa cggcgcgctc
     1621 gcctccgcgg ggttcttcga cccggccacc aacaacggta tctggctggc gggcgactac
     1681 atcggcaact atccctattt ccgcatcaac tcggtcaacg acgggctggt cgcccaggcg
     1741 gtgacgacgc ggcgcctcgc gcagatgtac acgttgctcc atgacgggac gctggtcggt
     1801 gggatcagca gctcggagat gcgggatctg ctggccaagg cggtcgcggt gccggaggtc
     1861 ttcatcaacc gtgcctcggg cctgaatttc accgtgaccc acacgaaggt gggcctcggc
     1921 cccctgaagc cgaagaacgg cgtcatcgac aacgtctatt ccgaggcatc gatcctccag
     1981 cacacgccgt cggggcggaa gttcgtggcc gtgtggcaga acttcgtctc cgggtccgat
     2041 ggattcgacc cgatcgggca cgtggtgcgc gacacgatcg acgcgttcct gaagccctga
     2101 gaccgcgagc tcgccacgcg cgccgcgatg tccgccggcc atcgagacgg cattcctgtc
     2161 ggtctgtcgc ggctcgcgtg gcccgcgccg gtatgatgcg ggagccatgg aaccctccgg
     2221 aggtggcacg cgcgcgccct ggctcgacgc gcggttcgat ctgacggcgc gcggcacgac
     2281 cgtccgccgc gagatcctcg ccggcgcgac cacgttcgtg accatggcgt acgtgctgtt
     2341 cgtcaacccg cagatcctcg ccgccgcggc cgggcccgag cgcctcggcg cgctgctgac
     2401 ctcgaccgcg ctggtcgcgg cggtgatgac cgtggccatg ggcgtggtcg ccaacctgcc
     2461 gctcgcgctc gcgcccggca tgggcctcaa cgcggtcgtc gcgttccagc tggtcggcgc
     2521 ggcgcacctg tcgttcgccg aggcgatggg cgtgatcgtc gccgagggcg tggtcatcac
     2581 gctgctcgtg gtcaccggcc tgcgccaggc gatggtgcgc tcggtgccgc tgccgctcaa
     2641 gcgcgcgatc ggcatcggca tcggcctgtt cctggcgctg atcggcttca agaacgccgg
     2701 cttcgtctcc gccggcggcg ggctgctcac cctgggcgac gccggccggc tgcacggctt
     2761 cccggtcgcg ctgttcgccg tgaccctggt gttcaccgcg tggctgatcg cccgcggcgt
     2821 gcgcggcgcg ctgctgatcg gcatggccgc gagcacgctc gccgccgtga tcgcgcgcgc
     2881 cgcgttcggc
//
LOCUS       DASVOR010000242         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353008,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000242 DASVOR010000000
VERSION     DASVOR010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353008"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VF516_05260"
     CDS             <1..876
                     /locus_tag="VF516_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107114.1"
                     /translation="IGALAVPAAHRIAYVARPRRKARELGVGVVDLEEVKTWRPIPLG
                     TARPISVAYSARPPLGLWIGTGAPRATLWRQLGDDYRLHPLAARTARPAGPWLEVTAR
                     AAHLHALPPDVTADWDEQSLASAIRIGTSNRVVSVPSPGLIDGNTAAWSPDRVRVAFV
                     AQLDDHCAAGAVNTAAFIADAAIGRTRELERAAGGIAIAWLTERTLAIAGDRGVSIHS
                     LDDGVAPIPIHGATDLIIPRERPRCTAPEPRDDAAEDAEPATESGTEAGTETAEIGEP
                     APGDEPPDAGVIDAR"
     gene            966..1361
                     /locus_tag="VF516_05265"
     CDS             966..1361
                     /locus_tag="VF516_05265"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEX8107115.1"
                     /translation="MRNTRELLASGFGGAVGTAVDVFVLVLMVQHGVAIAAAAFSGAA
                     AGAAMNFALNKYVAFRDRSPITSRQIVRFGIVAVATATLMAAAMHLVTVKLGVPYLLA
                     KLICAAVVFAAWTYPAQRRIVFRRPAHQV"
     gene            1377..>1737
                     /locus_tag="VF516_05270"
     CDS             1377..>1737
                     /locus_tag="VF516_05270"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8107116.1"
                     /translation="MSTSVPFVDLSIVVPAYNEEHRIAPTLARLSAFLTNQPMRWEIV
                     VVDDGSRDATCAVVEAARAHIPNLRLVRQTPNRGKGAAVRLGMREARGQIRVMSDADG
                     SMPPEQLPRLLAPILACQ"
BASE COUNT          235 a          694 c          572 g          236 t
ORIGIN      
        1 atcggcgcgc tcgccgttcc cgccgcgcat cgcatcgcct acgtcgcccg cccccggcgc
       61 aaggcgcgcg agctcggcgt cggcgtggtc gacctcgagg aggtcaagac ctggcggccg
      121 atcccgctcg gcaccgcccg gccgatctcc gtcgcgtaca gcgcccgacc gccgctcggc
      181 ctgtggatcg gcaccggcgc accgcgcgcg accctgtggc gccagctcgg cgacgactac
      241 cggctccatc cgctggcggc gcggaccgcg cgcccggccg gcccgtggct ggaggtcacc
      301 gcgcgcgcgg cccacctcca cgcgctgccg cccgacgtca ccgccgactg ggacgagcag
      361 tcgctcgcca gcgcgatccg gatcggcacc tccaaccgcg tggtctcggt gcccagcccc
      421 ggcctgatcg acggcaacac cgcggcgtgg tcgcccgacc gcgtccgggt cgcgttcgtc
      481 gcccagctcg acgaccactg cgccgccggc gcggtcaaca ccgccgcgtt catcgccgac
      541 gccgcgatcg gccgcacccg cgagctcgag cgcgccgccg gcggcatcgc gatcgcctgg
      601 ctcaccgagc gcaccctcgc gatcgccggc gaccgcggcg tgtcgatcca cagcctcgac
      661 gacggcgtcg ccccgatccc gatccacggc gccaccgacc tgatcattcc gcgcgagcgg
      721 ccgcgctgca ccgcccccga gccgcgcgac gacgcggccg aggacgccga gccggccacc
      781 gagtctggaa ccgaggcagg aaccgagacc gccgagatcg gggaacccgc gccgggtgat
      841 gaaccgccgg acgccggtgt catcgacgca cgctagtgcg gtgaagaact gacacgattt
      901 ccacggcaat tcagtcactt ggcattggtc ggacctctgg cacagcggct gctgtacctc
      961 gcagcgtgag aaacacccga gagctgcttg cctccggttt tggaggtgcg gtcggcaccg
     1021 cagtcgacgt cttcgtgctc gtcctgatgg tccagcacgg cgtcgcgatc gccgccgccg
     1081 ccttcagtgg agcggccgcg ggtgcggcga tgaacttcgc gttgaacaag tacgtcgcct
     1141 tccgggatcg ctcgccgatc accagccgtc agatcgtgcg cttcggcatc gtcgccgtgg
     1201 cgaccgcgac cctgatggcg gccgcgatgc acctcgtcac cgtcaagctc ggcgtgccgt
     1261 acctgctcgc caagctgatc tgcgccgccg tggtgttcgc ggcgtggacc taccccgcgc
     1321 agcgccggat cgtgttccgg cgcccggcgc accaagtctg agccaaggag ctgcccatgt
     1381 cgacctcggt cccgttcgtg gatctctcga tcgtcgtgcc cgcgtacaac gaggagcacc
     1441 ggatcgcgcc caccctcgcg cggctgtccg ccttcctcac caaccagccg atgcgctggg
     1501 agatcgtcgt cgtcgacgac ggctcgcgcg acgccacgtg cgccgtcgtc gaggccgcga
     1561 gggcccacat cccgaacctc cgcctggtcc gccagacccc caaccgcggc aagggcgccg
     1621 ccgtccgcct cggcatgcgc gaggcgcgcg gccagatccg cgtgatgagc gacgccgatg
     1681 gctcgatgcc gcccgagcag ctgccgcgcc tgctcgcgcc gatcctcgcc tgccagg
//
LOCUS       DASVOR010000243         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_528265,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000243 DASVOR010000000
VERSION     DASVOR010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528265"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF516_05275"
     CDS             <1..475
                     /locus_tag="VF516_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8107117.1"
                     /translation="LAPDSGLTAPTDDALARLIARACDGAIGFDELRKLGLLDLLDAQ
                     LGEHRALVDRLAPTHLRLPRRGRAPIHYELDQPPWIASRMQDFFGLARAPAVGDGRVP
                     LVLHLLAPNQRPVQVTTDLPGFWVKHYPALRKQLMRRYPKHAWPEDPTALITGDA"
     gene            complement(508..1248)
                     /locus_tag="VF516_05280"
     CDS             complement(508..1248)
                     /locus_tag="VF516_05280"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8107118.1"
                     /translation="MRNRVAPFVVVVASLAGCAENQGTPSAPETPTIGADRATVTGHV
                     LDTTGAPVVGATVTIRSSGEHATVDSTGAFTLDVPANTTLTLSATAPSMAPTLLQQFL
                     VSTGTAAQVEIPMITSDHFKSLAAMGMNPSGGVVAVAVKSLSGAGSAAGATVQLTPSL
                     GRVMYAPAGLGMADPDPAMAAVVPSVVQGDESYAWALGVQPHVSIMQFALHGVAEIEP
                     PYAIDDVIWPGTFTVDAGALTLVTLFTK"
     gene            complement(1813..2424)
                     /locus_tag="VF516_05285"
     CDS             complement(1813..2424)
                     /locus_tag="VF516_05285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8107119.1"
                     /translation="MTGHVLDTSGAPVAGALVAVRTSGEHATADATGAFTLDVPANTT
                     LTLSATAPNMAPTLLQQFLVAPGASGAVELPMVTSDHFKTLVGMGSNAMGGVVAVALK
                     SMSGAGSLAGATVELTPSLGRVMYAATGAGMSDPDPAMAMVVQGNDPYVWAVGVQPHV
                     SIMQLALRGVSQVEPPYSLDDVMWPGTFTVDAGALTLVTLFTK"
BASE COUNT          494 a         1127 c         1054 g          477 t
ORIGIN      
        1 gctcgccccc gacagcgggc tcaccgcgcc gaccgacgac gcactcgccc ggctgatcgc
       61 gcgcgcgtgc gacggtgcga tcggcttcga cgagctacgc aagctcggcc tgctcgacct
      121 gctcgacgcc cagctcggcg agcaccgcgc gctggtcgac cggctcgcgc cgacccacct
      181 gcggctgccg cgccgcggcc gcgcgccgat ccactacgag ctcgaccagc cgccgtggat
      241 cgcgtcacgc atgcaggact tcttcggcct ggcccgcgcg cccgccgtcg gcgacggccg
      301 cgtcccgctg gtcctccacc tgctcgcccc caaccagcgc ccggtccagg tcaccaccga
      361 cctgcccggg ttctgggtca aacactaccc cgcgctgcgc aagcagctga tgcggcgcta
      421 ccccaagcac gcctggcccg aggaccccac cgcgctgatc accggcgatg cctgaatgcg
      481 ggcgatctcg agccgcagcc cggagcccta cttcgtgaac agcgtgacca gggtgagcgc
      541 gccggcgtcg acggtgaacg tgcccggcca gatgacgtcg tcgatggcat acggcggctc
      601 gatctcggcg acgccgtgga gcgcgaactg catgatggag acgtggggct ggacgccgag
      661 cgcccaggcg taggactcgt cgccctgcac gaccgacggc acgaccgccg ccatcgccgg
      721 gtccgggtcg gccatcccga ggccggccgg cgcgtacatc acgcggccca ggctcggggt
      781 gagctggacg gtggcacccg cggcgctgcc ggcgcccgac agcgacttga ccgcgacggc
      841 gaccacgccg cccgaggggt tcatgcccat cgcggccagg ctcttgaagt ggtcgctggt
      901 gatcatcggg atctccacct gggcggcggt gccggtcgag accaggaact gctgcaggag
      961 ggtgggcgcc atgctgggcg cggtcgccga gagggtcagc gtggtgttgg cggggacgtc
     1021 gagggtgaac gcgcccgtgc tgtccacggt ggcgtgctcc ccggagctgc ggatggtcac
     1081 ggtcgctccc accaccgggg cgccggtggt gtcgaggacg tggccggtca ccgtcgcgcg
     1141 gtccgcgccg atggtgggcg tctcgggggc ggagggcgta ccctggttct ccgcgcaacc
     1201 cgcgagcgag gccaccacga cgacgaacgg agcgactcga ttcctcatga cggacaggcc
     1261 tcagtgcacg ggcaatgccg aagtcagggc gggcggcctg cgcgcgggac tgcgcccgaa
     1321 gatcgcgagt cttctcgaga gcttcttcga gctccggacg gtcggcggct cgatcgagag
     1381 cgaggcgagg gactcgcggc accgaccatc ctcgcggttc agcggcggca cgttcgcggc
     1441 gatcgatgcg acacctacct cgctgcgcgg tcgccgatga cgccctcgac cgcatggata
     1501 actgctcgca gtcccaagta attttcatcg agggcgcggc actcccgatt cgacgcttcg
     1561 tccccggacc gcgtttgcag cgcggttccc ctcgcatgtc cggggggcac ctcgaacgtc
     1621 cacccctggg cggttccatc cagcgagaac cacgcggcaa gtcatggagg atccgggcag
     1681 gtctcgctgc cggagctcgt gctcgggcgc gcagcaccgg cggcgctcgg ccccgcgcgc
     1741 ggccccggat cgggagctca ggagcggttc accccgcgcg ctggatcgcg cggaggaacc
     1801 tgctcgtgat tctcacttcg tgaacagcgt gaccagggtg agggcgccgg cgtcgacggt
     1861 gaacgtgccg ggccacatga cgtcgtcgag cgagtacggc ggctcgacct gcgatacgcc
     1921 gcgcagcgcg agctgcatga tcgacacgtg cggctgcacg ccgaccgccc agacgtacgg
     1981 atcgttgccc tgcacgacca tcgccatcgc cgggtccggg tcggacatcc cggcgcccgt
     2041 cgccgcgtac atcacccggc ccaggctcgg cgtgagctcg acggtggcgc ccgcgaggct
     2101 gccggccccc gacatcgact tcagcgcgac ggcgaccacg ccgcccatcg cgttgctgcc
     2161 catcccgacc agggtcttga agtggtcgct ggtgaccatc gggagctcga ccgccccgct
     2221 cgcgcccggc gcgaccagga actgctgcag cagcgtgggc gccatgttcg gcgcggtcgc
     2281 cgagagcgtc agcgtcgtgt tcgccgggac gtcgagcgtg aacgcgcccg tggcgtctgc
     2341 cgtggcgtgc tcaccggacg tgcgcacggc caccagggct cccgccacgg gcgcgccgct
     2401 ggtgtccaag acatggccgg tcacggtcgc tcgatcgttc ccggtggtag gtgtttcagg
     2461 ggcagtgtga ggtgccaggg ggtccgcgca accggtgagc gaggccacaa gcacgacaac
     2521 ccatgtgact cgttgatagt tactcatcta aaaatcccgt gtccttgatt acgatacagt
     2581 catcctagaa aaaactcgcc tccactgtca acgtcaactt actggtgact acacccattg
     2641 ctaagtgatt tttgtcacaa aacgagttcg caactagaat taatctcagg tggtacccat
     2701 agttaggcgc tgacgatcca cacagaaagc acttccgtat gggtgaaaaa aagcgcgaaa
     2761 aatacgtccg gaccccggga tcgcccgccc tgaacggctt cccgaccgct ggcatcgctt
     2821 cacgaatttt caatcactta cagcgatcgc gcctcgcgct cgatgcgatc taccgctgca
     2881 ggaaccacag ggccgcttac agctctcttc cacctcactc tcgactaccg gtgcggcagt
     2941 tccatatatg agacgttccc cgcccgcgtc gaccgtccgc ccggacggca cgactctcac
     3001 gatcgcctca cccgatcgat cgttgtcagg ccgcgcgcac ggcgcggcgc ccgcacggcc
     3061 gctcgctgcg accgacctcg accggcgatg cgcctggttc gttcccggtg agagctcgat
     3121 gccgccggcg tgcagcgcga agggcgcgac gg
//
LOCUS       DASVOR010000244         2630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_60716,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000244 DASVOR010000000
VERSION     DASVOR010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60716"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VF516_05290"
     CDS             complement(<1..1002)
                     /locus_tag="VF516_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8107120.1"
                     /translation="MVTRADPEMNAVGRVELAVPALRIEAVDAARLVALLEEPMCSLA
                     QLRHALHAHRLAGAESFAELLSMARMVGVERHGYQIETVHRVLRTLRGRALLSDEVGL
                     GKTIEALMVLLEYRVRGMANRVLVLAPPALVPQWVGELAAKAGIAARTTEREGDAVWR
                     GDGVVVASLATARMQRNAAAVQAEPWDLVIVDEAHRVKRRGSSSWKLVDGLRSRFLLL
                     LTATPIETELEELYQLVTLLKPGQFATPAAFRRRFVDPASPTSPKNREALRELLGEVM
                     VRNTRASCGLKLPPRYVSTVVVEPDDAERALYAAAMAAVRSAGEPGVRRTAGLLLLEA
                     "
     gene            1500..>2630
                     /locus_tag="VF516_05295"
     CDS             1500..>2630
                     /locus_tag="VF516_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107121.1"
                     /translation="MIVKHRQAPRSIASRFAHIAIATPHLLLAASACVEPPEEATAVL
                     SATVPNDQQYANSLGTVATYDTAGGVDLSGPFFQSLGSNGRSCASCHQLDQGMSIAPP
                     AIQARFTATRGLDPLFATVDGSNSPSADVSTLPARRAAFSMLLTKGLIRVGLAIPASA
                     EFTLSAVDDPYHFASASQLSLFRRPLPTTNLGFLATVMWDGRETIPGNTIDADLLDQA
                     NAATLTHAQAAVPLTAAQARAIVDFEKTLYTGQYRDGVAQELAAKGSNGGAAAPSDFL
                     ATQPFYPGINDPLGDPRTGAAFDPNVFHLYDAWASTPAGMGPKNDARRSILRGQTVFN
                     TRPIAITNVAGLNDNPAFGSPRVLRGTCTTCHDTPNVGNHSVG"
BASE COUNT          429 a          988 c          853 g          360 t
ORIGIN      
        1 ggcctcgagc aacagcagcc ccgccgtgcg acgcacgcct ggctcgccag cgctgcgcac
       61 ggccgccatc gccgcggcat acagcgcgcg ctcggcgtcg tccggctcga cgacgacggt
      121 cgatacgtag cgcggtggga gcttgaggcc gcacgaggcc cgcgtgttgc gcaccatcac
      181 ctcgccgagc aactcgcgca gcgcctcgcg gttcttcggc gaggtcgggc tcgccggatc
      241 gacgaaccgc cggcggaagg cggccggggt ggcaaactgc ccgggcttga gcagcgtcac
      301 gagctggtac agctcctcga gctcggtctc gatcggcgtc gcggtcagga gcagcaggaa
      361 ccggctgcgc agcccatcga cgagcttcca gctcgacgag ccccgccgct tgacccggtg
      421 ggcctcatcg acgatcacca ggtcccacgg ctcggcctgc acggcggcag cattgcgctg
      481 catccgcgcg gtcgcgagcg atgccacgac cacgccgtcg ccgcgccaca cggcgtcccc
      541 ttcgcgctcg gtggtgcgcg ccgcgatccc ggccttcgcg gcgagctcac ccacccattg
      601 tgggaccagc gccggcggcg cgagcaccag gacgcggttc gccatgccgc gcacccggta
      661 ctccaggagc accatcagcg cctcgatcgt cttgcccagg ccgacctcgt ccgacagcag
      721 cgcccgcccg cgcaacgttc gaagcacgcg atggaccgtc tcgatctggt agccgtgccg
      781 ctcgacgccg accatgcgtg ccatcgacag caactcggcg aagctctccg caccggcgag
      841 gcgatgagca tggagcgcat gacgcagctg cgcgagcgag cacatgggct cctcgagcaa
      901 cgcgaccaga cgcgccgcat cgacggcctc gatccgcagc gccggtacgg cgagctcgac
      961 tcgcccgacc gcgttcatct ccggatccgc tcgcgtgacc atcgccattc atctaccaca
     1021 cgcctctgat catcgcggcg ggagcctccg cgcccgacgg cgacgaggtg cgcagcgctg
     1081 cacgagtcac ccgagcagcg gccggacgcg ccatgcgagc gggcccgagg tgaagcccgg
     1141 ccggacgcca cttccccaca gctcgctgtg ctccggcgtc gcggcttcct ggctgcctga
     1201 caagatcgtg ccggccacac gacgaaaccc gctgttccat gacggacatc cgagctgccg
     1261 gtgcgctccg gggctaccgc ggcgctcgat ccacgatgcg agtgcaggcg cgggcgaggt
     1321 gcggcgcgcg gccgcgatcc gcgacccgag ctccctcgtc tcatgtggcc gacgaccgga
     1381 cagcagaaat gagagtgcat gactcgcagt gatgaggcat tcacgatcga acaacgaaaa
     1441 atcggagtgc gtgactcatg gcgatggagt aatccgcggc gatgaggcat tcattgacca
     1501 tgatcgtgaa acaccggcag gcgccccgat cgatcgcgtc ccgcttcgcg cacatcgcga
     1561 tcgcgacgcc gcacctgctg ctcgcggcga gcgcgtgcgt cgagccaccc gaggaggcca
     1621 cggcggtgct cagcgccacg gtgccgaacg accagcagta cgcgaactcg ctcggcacgg
     1681 tcgcgaccta cgacaccgcc ggcggggtcg atctgtccgg cccgtttttc cagtcgctcg
     1741 gcagcaacgg gcgaagctgt gccagctgcc atcagctcga ccagggcatg tcgatcgcgc
     1801 cgccggcgat ccaggcgcgg ttcaccgcga cccgaggtct cgatccgctg ttcgcgacgg
     1861 tcgatggctc caactcaccc agcgcggacg tctcgaccct ccccgcccgg cgggccgcct
     1921 tcagcatgct gttgaccaag ggcttgatcc gcgtcggcct ggccatcccc gccagcgccg
     1981 agttcacgct cagcgccgtc gacgacccgt atcacttcgc gagcgcgagc cagctgtcgc
     2041 tgtttcgccg cccgctgccg acgaccaacc tcggattcct ggcgaccgtg atgtgggacg
     2101 gccgcgagac gatccccggg aacaccatcg acgccgacct gctggatcag gccaacgccg
     2161 ccacgctgac ccatgcccag gcggccgtgc cgctgaccgc cgcgcaggcg cgcgcgatcg
     2221 tcgacttcga gaagacgctg tacaccggac agtaccgcga cggcgttgcg caggagctgg
     2281 cggcgaaggg ctcgaacggc ggcgcggcgg ccccctcgga cttcctcgcc acacagccgt
     2341 tctatcccgg catcaacgat ccgctgggag acccgcggac cggcgcagcg ttcgacccga
     2401 acgtgtttca cctctacgac gcgtgggcga gcacgcccgc cggcatgggc ccgaagaacg
     2461 atgcgcggcg atcgatcctg cgtggccaga cggtgttcaa caccaggccg atcgcgatca
     2521 ccaacgtcgc ggggctcaat gacaaccccg cgttcggctc accgcgggtc ttgcgtggca
     2581 cctgcacgac ctgccacgac acccccaacg tgggcaatca ctcggtcggc
//
LOCUS       DASVOR010000245         4271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_499171,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000245 DASVOR010000000
VERSION     DASVOR010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499171"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="VF516_05300"
     CDS             complement(<1..244)
                     /locus_tag="VF516_05300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107122.1"
                     /translation="MAEPAAAVDPAARGLGPKIKNRWRAWLRAIHRDIGYLAVGFTLI
                     YALSGIAMNHIDDWDPSFHSSERVLKIKPVADDASDD"
     gene            complement(237..659)
                     /locus_tag="VF516_05305"
     CDS             complement(237..659)
                     /locus_tag="VF516_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8107123.1"
                     /translation="MPERAIDPAGADPSIAASSPAPASATARRAARSGGIAAAIRKRW
                     RAWLRAVHRDIGYLAVGFTVIYALSGIAMNHIDDWDPNFHATEHGAPVRGLIPDLYFW
                     KSAKWITGVRFVKDDKPGYWEVRGYHNHADPWKEERYG"
     gene            complement(652..1386)
                     /locus_tag="VF516_05310"
     CDS             complement(652..1386)
                     /locus_tag="VF516_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8107124.1"
                     /translation="MRDPRRPDLDRYPELKKAWDRRQFIRSAAVGTAFMAFGGALVRI
                     AGDDLSKEARAEKRTDGKDRLPPGQRALQALRPMGGAEGDGDARNFRLRVHGAVKSPF
                     EIDYAGLLQLPQVEKEADVHCVTGWSLLGGLWKGVQIATLAEKAQVKSDARFVIFEAA
                     HGYTANAPLKEATADTAMVTYRLNGKPLALQHGAPVRGLVPDLYFWKSAKWITGVRFV
                     RNDEPGYWEVRGYNNHADPWKEERYA"
     gene            complement(1506..2138)
                     /locus_tag="VF516_05315"
     CDS             complement(1506..2138)
                     /locus_tag="VF516_05315"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8107125.1"
                     /translation="MQGRHVVVTGGKGALGGAVVAWLEARGAIVHVPDIATVDLSNEA
                     QATAYYAALPPLWASIQLAGGFAMAPIADTALADFEQQWRMNTVTCFLSCREAVRSIR
                     RAGGGGRIVNVAARPVVAPVPGMTAYVASKAGVAAITQALATEVAADRILVNAVLPSI
                     IDTPANRAAMPSADPAAWPKPAEIAETIGFLASPGNALTSGSLVPVYGRA"
     gene            2379..4049
                     /locus_tag="VF516_05320"
     CDS             2379..4049
                     /locus_tag="VF516_05320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107126.1"
                     /translation="MLSDATQADPSTATEVGTQAASPHAIAEVASGSRHPEQDARPSG
                     LGTASRQVATAAEALDRTDLRRMRSFHIFGIIASIGAIALSTFLGGDPTYRHLFWIGV
                     SVLGACNVVLLYLTTSPDLYRPVPVGILWLLSNAGFIPAILYFGPYSAAVMVHMLALL
                     FISLGRLRWPAIATAVIGIGGHLAITLPIMLGWIRDRGILTSRFTEHHQLWVAEILVC
                     GFMASGYGLGRWARSLNAKALAELQQAMRVIGDKEQALAEVVDQARRANHANEGRWTH
                     QVMGSFKLGLVLGRGAMGEVYEATRSDGTPAAVKLLNARSTASSTLVERFHREMAVAA
                     RLESPHIVRVFELSRADAPVPYLAMERLHGTDLATRLRAETRLPSDELVAMIDQVARG
                     LEVARIAGVVHRDLKPHNLFFHGGATWKILDFGVSKVLGSEGTLTGEGIVGTPQYMAP
                     EQASGGQVTHAADIYALGAIAYRCLTGRSPFKGRDLSELVYQVVHAAPMRPSALGRVS
                     PVIEDVLAIAMAKDPRRRFPSAHAFAAAFIAARRGRPAPIEPPSDAWS"
     gene            4073..>4271
                     /locus_tag="VF516_05325"
     CDS             4073..>4271
                     /locus_tag="VF516_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX8107127.1"
                     /translation="MFRMPAEWELHDAVWTAWPHDPDQWLEGLEAPQRALMAMVAAIA
                     DLDGGRPRGERVELLVRDARDE"
BASE COUNT          597 a         1578 c         1459 g          637 t
ORIGIN      
        1 cgtcatcgga ggcatcgtcg gcgaccggct tgatcttcaa gacgcgctcg ctcgagtgaa
       61 agctcggatc ccaatcgtcg atgtggttca tcgcgattcc ggagagcgcg tagatcagcg
      121 tgaagccgac cgcgagatag ccgatgtctc gatggatcgc gcgcagccat gcgcgccagc
      181 ggttcttgat cttcgggccg aggcctcgag cggccgggtc gacggccgcc gcgggctcag
      241 ccatagcgct cttccttcca cgggtcggcg tggttgtgat agccgcgaac ttcccagtag
      301 ccgggcttgt cgtccttgac gaagcgcacg ccggtgatcc acttcgcgct cttccagaag
      361 tagaggtcgg ggatcaggcc gcgcaccggt gcaccgtgct cggtggcgtg gaagttggga
      421 tcccagtcgt cgatgtggtt catcgcgatc cccgacagcg cgtagatcac ggtgaacccg
      481 acggcgaggt agccgatatc gcgatggacc gcgcgcaacc atgcccgcca gcgcttgcgg
      541 atcgccgccg cgatgccgcc gctgcgcgca gcgcgcctgg cggtggcaga ggctggagcg
      601 gggctggaag cggcgatcga cgggtcggca ccggccgggt cgatcgcccg ctcaggcata
      661 gcgctcttcc ttccacgggt cggcgtggtt gttgtagccg cggacctccc agtagcccgg
      721 ctcgtcgttg cggacgaacc gcacgccggt gatccacttg gcgctcttcc agaagtacag
      781 atccggcacc aggccgcgca ccggcgcgcc gtgctggagc gccagcggct tgccgttgag
      841 ccggtaggtc accatcgcgg tgtcggcggt cgcctccttg agcggcgcgt tcgcggtgta
      901 gccgtgcgcg gcctcgaaga tcacgaaccg cgcgtccgac ttgacctgcg ccttctcggc
      961 gagcgtggcg atctgcacgc ccttccacag cccgccgagc agcgaccagc cggtcacgca
     1021 gtggacgtcg gcctccttct cgacctgcgg cagctggagc aggccggcgt agtcgatctc
     1081 gaacggcgac ttgaccgcgc cgtgcacgcg cagccggaaa ttgcgggcgt cgccgtcgcc
     1141 ctccgcgccg cccatcggcc gcagcgcctg cagcgcgcgc tgcccgggcg gcagccggtc
     1201 cttgccgtcg gtccgcttct cggcgcgcgc ctccttcgag aggtcgtcgc cggcgatccg
     1261 gacgagcgcg ccgccgaacg ccatgaacgc cgtcccgacc gcggcggacc ggatgaactg
     1321 cctgcggtcc caggccttct tcagctcggg ataacgatcc aggtcgggtc ggcgggggtc
     1381 gcgcacaggt cctccttgcg ccggcagcgt agccgatcag gcatccgcag ccggtgctct
     1441 atgtacgttg taccgagcgc gccagttacg cgcttccgga ccgcgtaagt ccccgaaaga
     1501 tgcggtcagg cccggccgta caccggcacc agcgaccccg aggtcagcgc attgcccggc
     1561 gacgccagaa acccgatggt ctcggcgatc tccgccggct tgggccacgc cgcagggtcg
     1621 gccgacggca tcgccgcccg gttggccggc gtgtcgatga tcgatggcag caccgcgttg
     1681 accaggatgc gatcggcggc gacctcggtg gccagcgcct gcgtgatcgc cgcaaccccg
     1741 gccttcgacg cgacatacgc cgtcatgccc ggcaccggcg cgaccaccgg gcgcgcggcg
     1801 acgttgacga tccgcccacc gccgcccgcg cggcggatcg agcgcaccgc ctcgcggcac
     1861 gacaggaagc aggtcaccgt gttcatccgc cattgctgct cgaagtcggc cagcgcggtg
     1921 tcggcgatcg gcgccatcgc gaacccgccg gcgagctgga tcgatgccca cagcggcggg
     1981 agcgcggcgt agtacgcggt ggcctgcgcc tcgttcgaaa gatccacggt ggcgatgtcc
     2041 ggcacgtgga cgatcgcgcc cctggcctcc agccaggcga ccacggcgcc tccgagcgcc
     2101 cccttcccgc cggtgacaac gacatgacgg ccttgcatcg cgcgcgacca taccccaggc
     2161 ccgcggatcc ccaaggctcg cgaccggttc catcccgacg cgctctgcga cggtttcgct
     2221 acgctcgatc ccacgggggc tccgccccca ccccgccggc gaagccgtcg ggggtcccca
     2281 tccccgtctc gctacgctcg aagcgtaggt ttacacgtac gtttgcacac tgcagatcat
     2341 tccagcccca gcggcaagac gcgctactat tcgcggcggt gctgtccgac gccacacagg
     2401 ccgacccgtc gacggcgacg gaggtcggca cgcaggcagc ttccccgcac gcgatcgccg
     2461 aggtcgcctc cggatcgcgc catccggagc aggacgcccg gccatccgga ctgggcaccg
     2521 catcgcgcca ggtcgcgacc gccgccgagg ccctcgatcg caccgatctg cggcggatgc
     2581 ggtcgttcca tatcttcggg atcatcgcct cgatcggcgc gatcgcgctg tcgaccttcc
     2641 tcggcggcga tccgacctac cgccacctgt tctggatcgg cgtcagcgtg ctcggcgcgt
     2701 gcaacgtcgt gctgctctac ctgaccacga gccccgacct ctaccgcccg gtcccggtcg
     2761 ggatcctgtg gctcctgtcc aacgccggct tcatccccgc gatcctgtac ttcggcccgt
     2821 actcggccgc ggtcatggtc cacatgctcg ccctgctgtt catctcgctg gggcggctgc
     2881 gctggccggc gatcgcgacc gcagtgatcg gcatcggcgg ccacctcgcg atcacgctgc
     2941 cgatcatgct gggctggatc cgcgatcgcg gcatcctgac cagccggttc accgagcacc
     3001 accagctgtg ggtcgccgag atcctggtct gcggcttcat ggcctcgggc tacggcctgg
     3061 gccggtgggc gcgcagcctc aacgccaagg cgctcgccga gctccagcag gcgatgcgcg
     3121 tgatcggcga caaggagcag gcgctcgccg aggtcgtcga ccaggcgcgg cgcgccaacc
     3181 acgccaacga gggccggtgg acccaccagg tcatgggcag cttcaagctc ggcctcgtgc
     3241 tcggccgggg cgcgatgggc gaggtctacg aggcgacgcg cagcgacggc acgcccgccg
     3301 cggtcaagct gctcaacgcg cggtcgaccg cgagcagcac cctggtcgag cggttccacc
     3361 gcgagatggc ggtcgcggcg cgcctggagt cgccccacat cgtccgcgtg ttcgagctgt
     3421 cgcgggccga cgcgccggtg ccgtacctcg cgatggagcg cctgcacggc accgacctcg
     3481 cgacccggct gcgcgccgag acccggctgc cgtccgacga gctggtcgcg atgatcgacc
     3541 aggtcgcgcg cggcctcgag gtcgcccgga tcgccggcgt cgtccaccgc gatctcaagc
     3601 cgcacaacct gttcttccac ggcggcgcga cctggaagat cctcgacttc ggcgtgtcca
     3661 aggtgctcgg cagcgagggc acgctgaccg gcgagggcat cgtcggcacg ccgcagtaca
     3721 tggcgcccga gcaggcgtcc ggcggccagg tcacccacgc cgccgacatc tacgcgctcg
     3781 gcgcgatcgc ctatcgctgc ctgaccggcc ggtcgccgtt caagggccgc gacctgtccg
     3841 agctggtcta ccaggtcgtc cacgccgcgc cgatgcggcc cagcgcgctc ggccgggtct
     3901 cgccggtgat cgaggacgtg ctcgcgatcg ccatggccaa ggacccgcgc cggcggttcc
     3961 cgtcggccca cgcgttcgcc gccgcgttca tcgccgcccg ccgcggccgc cccgcgccga
     4021 tcgagccgcc gtccgacgcc tggagctgag catctggtaa gacaccggcc tgatgttccg
     4081 aatgccggca gaatgggagc tgcacgacgc ggtgtggacc gcgtggccgc acgatccgga
     4141 ccagtggctg gagggcctcg aggcgccgca gcgcgcgctg atggcgatgg tcgcggcgat
     4201 cgccgacctc gacggcgggc gaccgcgcgg cgagcgggtc gagctgctgg tgcgcgacgc
     4261 ccgcgacgag g
//
LOCUS       DASVOR010000246         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148317,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000246 DASVOR010000000
VERSION     DASVOR010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148317"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1230)
                     /locus_tag="VF516_05330"
     CDS             complement(<1..1230)
                     /locus_tag="VF516_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107128.1"
                     /translation="MAHDPRPSSSTAAADDEPSDIGIGDDILIVDDTPANLVAFEAAL
                     APLGRHLVVAHSGTEALARLLDQDFAVIVLDVAMPDMTGIDTARLIRARGRSRGTPII
                     FVTGMAWEDAAIDEAYGAGGFDFVIKPVRPELLRAKVQVFLQLQERTRALRRQADQLR
                     ASQALLYEHELIEQRKRFESELLDSKLQQLSEGDRRQHELAALIGNELINPLHNLRVA
                     FDLLREHPHADRGERIYTRIEHWLEHVTRLVEGLIDVARLAAGQLAITPESVHLTELV
                     RQALDGCAGAIAARHLAVRIDADPAPALIVLGDPVRLLQAIAVVIDHAVRSAEDGGEL
                     AIAAAVASGDVVLRIRYAGRGFGPGVLRRLFDMYAPRDEDDDEGADAGRLRLEFALAR
                     RLIELHDGSIRASSDGPA"
     gene            1633..2649
                     /locus_tag="VF516_05335"
     CDS             1633..2649
                     /locus_tag="VF516_05335"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX8107129.1"
                     /translation="MTRTVLTGIKPTGAPHLGNLLGAIRPALRLAGDPALRAFYFIAD
                     YHALNSVHDRRELTRLTREVAATWLAFGFDSVLGSDRVLFYRQSDVPEVFELTWILAC
                     FTAKGWMNKAHAYKAKTAENERAGNPDLDAGIGMGLYLYPILMAADILLFEADLVPVG
                     EDQKQHIEIARDIAQRVNHTYGAELLKLPSALIDAGTAVIPGRDGRKMSKSYDNTIPL
                     FAPPAALRKLVNSIVTDSTPPEAPKDPASSVVFKIFEQFAPPDAVLALRRRYQDGISW
                     GEAKAALLAVLEPQLAEPRERYQTLIADPRRIDDLLALGRDRARALARPVIDRLRAAT
                     GIAG"
     gene            2886..3230
                     /locus_tag="VF516_05340"
     CDS             2886..3230
                     /locus_tag="VF516_05340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107130.1"
                     /translation="MDDEVDVLEMIELGLRSAGYNLLTADSGERALELFEQHRADLVI
                     CDLKMPRMNGIATISRLREQAPGLPIVMLTGYLTPETIEECTELGGVELLRKPFLFRE
                     LSKAVKVALKRG"
     gene            3390..>3820
                     /locus_tag="VF516_05345"
     CDS             3390..>3820
                     /locus_tag="VF516_05345"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8107131.1"
                     /translation="MTVSRHDRFGAVIAAGQRITQARLADEVRTALSDSARALLGAER
                     CSIIDHADGEADAPLVSAAFRSGHLVVWPADDATIQAPASGPPGVRCALCAPIPVSGG
                     LAAVLYATHSVEAAFGDDARAIAAFLVGLAGAALDSAAARSR"
BASE COUNT          561 a         1339 c         1364 g          556 t
ORIGIN      
        1 cgcggggccg tcgctcgatg cgcggatcga gccgtcgtgc agctcgatca gccgccgggc
       61 gagcgcgaac tcgagccgga gccgcccggc gtccgcgcct tcgtcgtcgt cctcgtcgcg
      121 cggcgcgtac atgtcgaaca gccgccgcag cacgccgggg ccgaagccgc ggccggcgta
      181 gcggatccgc agcaccacgt cgccgctggc gaccgcggcg gcgatcgcca gctcgccgcc
      241 gtcctcggcc gagcgcacgg cgtggtcgat cacgaccgcg atcgcctgga gcagccggac
      301 cggatcgccg agcacgatca gcgcgggcgc ggggtccgcg tcgatccgca cggcgaggtg
      361 gcgggcggcg atcgccccgg cgcagccgtc gagcgcctgg cggaccagct cggtcaggtg
      421 gaccgactcg ggcgtgatcg cgagctggcc ggcggcgagc cgcgccacgt cgatcaggcc
      481 ctcgaccagc cgggtgacgt gctcgagcca gtgctcgatc cgggtgtaga tccgctcgcc
      541 gcggtcggcg tggggatgct cgcgcagcag atcgaacgcc acgcgcaggt tgtgcagcgg
      601 gttgatcagc tcgttgccga tcagcgccgc gagctcgtgc tggcgccgat cgccctcgct
      661 cagctgctgg agcttggagt ccagcagctc ggactcgaac cgcttgcgct gctcgatcag
      721 ctcgtgctcg tagagcagcg cctgcgacgc gcgcagctga tcggcctggc ggcgcagcgc
      781 ccgggtccgc tcctggagct ggaggaacac ctggaccttg gcgcgcagca gctcggggcg
      841 gaccggcttg atcacgaagt cgaagccgcc cgcgccgtac gcctcgtcga tcgccgcgtc
      901 ctcccaggcc atgccggtca cgaagatgat cggcgtcccg cggctgcgcc cgcgcgcccg
      961 gatcagccgc gcggtgtcga tcccggtcat gtccggcatc gcgacgtcga gcacgatcac
     1021 cgcgaagtcc tggtcgagca gccgggcgag cgcctcggtg cccgagtggg ccacgaccag
     1081 gtggcggccg agcggcgcca gggcggcctc gaacgcgacc aggttggccg gtgtatcgtc
     1141 gacgatcagg atgtcgtctc cgataccaat atcggacggt tcgtcgtccg cggcggcggt
     1201 tgacgacgaa ggccgggggt cgtgcgccat cacgaaggcc agcccgggag agggggcgag
     1261 cgacggcgag aaaggccgct tgtcattgcg tcgccgacgc gcaaaagcaa gcgccgtgcg
     1321 cgcgcgcgga cgcgcggtcc cgcgaagcta gccaggccgg gctgtagcgc cgcggcacat
     1381 cgcgacccag cgcacgtccg attttttcac ggggtggcgc ggggagcggc cgccccgggc
     1441 gctcgctgcg gggggttaga tccgcgtgat cagaggcgct cgctccggcg agcgcttgct
     1501 tccgggggaa ccaccgagga atctcgatgt gatcgatgtc gcaccagcgg gcagcggcgc
     1561 acggcgtttg ccggccacgg agccgccggc ccggtcccgg gctcgctgcg gtgcggtacg
     1621 acttccggac gcatgaccag gaccgtgctc accgggatca agccgaccgg ggcgccgcac
     1681 ctcggcaacc tgctcggcgc gatccggccg gcgctgcggc tggccggcga ccctgcgctg
     1741 cgcgcgttct acttcatcgc cgactaccac gccctcaact ccgtgcacga ccgccgggag
     1801 ctgacccggc tgacccgcga ggtcgcggcg acctggctgg cgttcggctt cgattccgtt
     1861 ctcggctcgg accgcgtgct gttctaccgc cagtccgacg tccccgaggt gttcgagctg
     1921 acctggatcc tggcctgctt caccgcgaag ggctggatga acaaggccca cgcctacaag
     1981 gcgaagaccg cggagaacga gcgcgccggc aacccggatc tcgacgccgg tatcgggatg
     2041 gggctctacc tgtatccgat cctgatggcc gccgacatcc tgctgttcga ggccgacctg
     2101 gtcccggtcg gcgaggacca gaagcagcac atcgagatcg cgcgcgacat cgcacagcgg
     2161 gtcaaccaca cctacggcgc ggagctgctc aagctgccga gcgcgctgat cgacgccggc
     2221 accgcggtca ttcccggccg cgacggccgc aagatgagca agagctacga caacacgatc
     2281 ccgctgttcg cgccgccggc ggcgctgcgc aagctggtca actcgatcgt caccgactcg
     2341 accccgccgg aggcgcccaa ggaccccgca tcgtcggtgg tgttcaagat cttcgagcag
     2401 ttcgcgccgc ccgacgccgt cctcgcgctg cgccggcgct accaggacgg catctcgtgg
     2461 ggcgaggcca aggccgcgct gctcgcggtc ctggagcctc agctcgccga gccgcgcgag
     2521 cgctaccaga cgctgatcgc cgacccgcgg cggatcgacg acctgctggc gctgggccgc
     2581 gatcgggcac gcgcgctggc ccggccggtg atcgaccgcc tgcgcgccgc gaccggcatc
     2641 gcggggtgag ggtccggaac cggcagtgat gcctcacgaa tgagacagca tgttggcgcg
     2701 gatgttcttg tgccgcgcac cggatccgcg gtacctcaga aagtacggca cgcttccatg
     2761 cagcaaaatg ctgcgtggag gtcatgcctc cccattccgt atgctgtttg catgtctgtc
     2821 gaggtcctga tgaccagagg ggcccgggtg cattgatggc cgagccacgc ggtacgatcc
     2881 tggtcgtgga cgatgaggtc gacgtcctgg agatgatcga gctcggactc cgatccgcgg
     2941 gctacaacct gctcaccgcc gacagcggcg agcgcgcgct cgagctgttc gagcagcatc
     3001 gcgccgacct ggtgatctgc gatctcaaga tgcctcgcat gaacgggatc gcgacgatct
     3061 cgcggctgcg cgagcaggcc ccgggacttc cgatcgtcat gctcaccggg tatctgaccc
     3121 cggagaccat cgaggaatgc accgagctcg gcggcgtcga gctgctgcgc aaaccattcc
     3181 tgttccgtga actatccaag gcggtgaagg tcgcgttgaa gcgcggttaa gccgggttag
     3241 gggaaccaca cctgcgatgc tagtttcatg gtccacatgg ctgtgggggc gctcgggtcg
     3301 acgcgccgat ccaatcgtcg ctgcgtttgc ggcgtacatg aaaccacacc ggcacgccgg
     3361 gatgggggcc acgtgaacga ggggagcggg tgacggtgtc acgccacgat cgattcggcg
     3421 ccgtcatcgc ggccggccag cgcatcacgc aggcccggct cgcagacgag gtccgcacgg
     3481 cgctctccga cagcgcgcgc gcgctgctcg gagcggagcg ctgctcgatc atcgatcatg
     3541 ccgatggcga ggccgatgcg ccgctggtgt ccgccgcgtt ccggtcaggc cacctggtgg
     3601 tctggccagc cgatgacgcc acgatccagg ccccggcctc gggtccaccc ggggtccggt
     3661 gtgcgctgtg cgcaccgatc ccggtgagcg gcggcctggc cgcggtgctc tacgcgaccc
     3721 actcggtcga ggcggcgttc ggcgacgatg cgcgcgcgat cgcggcgttc ctggtcgggc
     3781 tcgccggcgc cgcgctggac agcgccgcgg cccgcagccg
//
LOCUS       DASVOR010000247         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265179,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000247 DASVOR010000000
VERSION     DASVOR010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265179"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(255..998)
                     /locus_tag="VF516_05350"
     CDS             complement(255..998)
                     /locus_tag="VF516_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8107132.1"
                     /translation="MRSSRSNRKPRRCAQQELFRRGGKRRGAGRKPKGARAGERHGAR
                     PEFKSYHPLHVVMRMAPEVGSLRRRTMYKALRDATITAALRERIRIVHISLQRTHVHM
                     LVEADHKAALARGMQGFTIAAARNINTALGNGARRRRGKVFADRYHVEVITTPTRARH
                     AISYILSNWRKHGEDRDGLPSTWLVDPFSSGISFPDWQELEGQPWMWPVRETYDPLVV
                     RRPRSWLLREGWKLVGGPISARDVPSKPR"
     gene            1716..1931
                     /locus_tag="VF516_05355"
     CDS             1716..1931
                     /locus_tag="VF516_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107133.1"
                     /translation="MIDIESLARAAEAAADALPSPANDRQRLLFGRIQSLVTKTSEQA
                     GASLRFANAQVTALTAQLEARRGAAGR"
     gene            complement(2703..>3108)
                     /locus_tag="VF516_05360"
     CDS             complement(2703..>3108)
                     /locus_tag="VF516_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107134.1"
                     /translation="GLGTVYYARAHTAEITGVLALAPYLGEPALIREIDDQGGLAGWQ
                     APPRVSVMTEDNYQRELWRWLQAVTRGQEPGPDLYLGFGMSDKLAFEDELLAQALPDD
                     HTFLIPGAHDWKTWRRLFDQFLDDSDFARRCR"
BASE COUNT          519 a         1100 c          987 g          502 t
ORIGIN      
        1 gatctcgcgg atcagcgccg gctcgccgag gtagggcgcg agcgcgagca cgccggtgat
       61 ctcggcggtg tgggcgcgcg cgtagtacac cgtgcccagc ccgcccatcg agttgccgac
      121 cagccagatc tcggcgtagc cgcgcgcgcg cgccggatcg acgacgtcgg ccgcgagccg
      181 cgggaggaac gtgccgcgcg cgtagtagcc gaccgtggca tgggccgcag tccacgccgg
      241 ggggagcgta cccttcagcg cggtttgctc gggacatcgc gtgcgctgat cggacctccg
      301 acgagcttcc agccctcgcg gagcaaccag ctccggggcc tccgcaccac gagcggatcg
      361 tacgtctcgc ggaccggcca catccaaggc tggccttcca gctcctgcca gtcggggaac
      421 gagatcccgc tggagaacgg gtccacgagc cacgtgctcg gcagtccatc gcggtcttcc
      481 ccgtgctttc tccagttcga aaggatgtaa gaaattgcgt ggcgcgcgcg ggtcggcgtc
      541 gtgatcacct cgacgtgata gcgatcggca aacaccttgc cacggcgccg gcgcgccccg
      601 tttccgagcg cggtattgat gttcctcgcc gccgcgatcg tgaagccctg catcccacgg
      661 gcaagcgccg ccttgtgatc ggcctcgacc agcatgtgaa cgtgcgtgcg ctgcaggctg
      721 atgtggacga tccggatccg ctcgcgcagt gccgccgtga tcgtggcatc gcgcagcgcc
      781 ttgtacatcg tgcgccgccg caagctcccc acctccggcg ccatccgcat cacgacgtgc
      841 agcggatgat acgacttgaa ctccggccgc gctccgtgcc gctcgccggc acgtgccccc
      901 ttgggcttcc gcccggcacc gcggcgcttg ccaccgcggc gaaacagctc ctgctgagcg
      961 cagcgccttg gcttgcggtt tgacctcgac gacctcatgg cgcgttcata accatgaacg
     1021 acgatcctgt caatgattgg gcacgactaa ttgccattca tacctgatgg ttaccaatcc
     1081 ttgaacagat cgcagaccaa gtgtccgaag caatacaact gttctcaaac agagacaggt
     1141 ccatcacgga gcgcgtacag caccgtgaac gcgtcgctcg ttgcgagcag accgaggcga
     1201 cgccaagcga cggatccccg gtgggtcatg acctgcctga tcgtgccagc gttcgacgaa
     1261 tcctcgaacg agaccttccg cagaacatga ggacggtctg tggagaacag ggacgcctga
     1321 tgacccgcgc cgcggcatga cgggtcgacc atggcgcggc tcacagtcgt ccttctgttc
     1381 cccacagccc ttgccctcga ctccaggcct ctaatttgca attagatatt attgtccttt
     1441 attgagaaag gaggatcgag catcagctca accacatctt gcaattcgcg gttgactcat
     1501 cgatctggcc ctgcgataca gaagcgttcc tatctggaac aaggccgcgc cgccaccggc
     1561 gcaagacatg tccatagcgg cccgccggtg ttagcgcacc gacgagccca ggagccgcaa
     1621 tgtccaagca tcgcagcacc caggtcaggg tatcgaacgc cccccagaac agcaacttcg
     1681 aagccggcac cgcgctggag aacctccgcg cggagatgat cgacatcgag tccctcgcgc
     1741 gcgccgcgga ggccgccgcg gatgcgctgc catcgccggc gaacgatcgc cagcgcctgc
     1801 tgttcggccg gatccagtcg ctcgtcacga agacttcgga gcaggctggc gcatcgctcc
     1861 gcttcgcgaa cgcgcaggtc accgcgctga cggcgcaact cgaggcgcgc cggggtgcgg
     1921 caggtcgttg aagtcccagc caggaccgcg aagacccggc tgagaccact cattgccgcg
     1981 gatgatgatc gtcccggttg ggaccgcaca ctgaccggag aacatcagtc ccagctggga
     2041 ccatgcacta tccggtgcga cccagtccca gctgggacca tgcacgggtc cgacgaacgt
     2101 cccttccagc cggaccgctc actgcgcggg tgaatccagc ctcctggacc cgccggtcga
     2161 cggctcgagc ctcggccact cggcgcgaag cccatctgtg ccgagcgcgc cttctccgac
     2221 gatccggaga taaaatacat ccggaaatgg ggcgtccgcg cggggcgatg cactgtgagt
     2281 gggccgtcct ggtccgcctg atccgctttg acgggccagt cacagggccg tccacgcggt
     2341 ctcccgtcgt ccctgggctg cagctgccgt gcggcgatgg cggtgattca acgctgccct
     2401 gcggagcgcg atccaggatc gccatcccgc tccctgacgg cagaccgtcc acgcgatctc
     2461 ccgtcgcccc tggccgcagc tgccgtgcgg cgatggcggt gattcaacgc tgccgcctgg
     2521 ctgccctgcg gagcgcgatc caggatcgct atccagttca cccgacggca gatcgtccac
     2581 gccgtcccct cattccctgg ctgcagcggc cgtgcggcga ctgcggcgat ccaacgccgc
     2641 cgccttgcct agcccgcccc cgcgctcgcc gcccccacgt gggggcgcgt cctcctgcac
     2701 ttctaccggc agcgccgggc gaagtccgag tcgtcgagga actgatcgaa caatcggcgc
     2761 caggtcttcc agtcatgcgc gccggggatc aggaacgtgt ggtcgtccgg cagcgcctgg
     2821 gcgagcagct cgtcctcgaa cgcgagcttg tcggacatgc cgaagccgag gtagaggtcc
     2881 gggcccggct cctggccgcg ggtcaccgct tgcaaccagc gccacagctc gcgctggtag
     2941 ttgtcctcgg tcatcacgga gaccctgggc ggcgcctgcc agccggcgag cccgccctgg
     3001 tcgtcgatct cgcggatcag cgccggctcg ccgaggtagg gcgcgagcgc gagcacgccg
     3061 gtgatctcgg cggtgtgggc gcgcgcgtag tacaccgtgc ccagcccg
//
LOCUS       DASVOR010000248         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_119222,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000248 DASVOR010000000
VERSION     DASVOR010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119222"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VF516_05365"
     CDS             complement(<1..1352)
                     /locus_tag="VF516_05365"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107135.1"
                     /translation="MNRRSRCLPVVILAAAVVSSSAAAQRGPGGGDRAPERPRFQLLG
                     PSSGGRISAAAGIPGDTMTYYFGAASGGVWKTTDGGNTSVPVSDSMPVQAIGALAVSP
                     SAPQIVWAGTGEAWAIRDADVMGDGIYKSSDAGKTWVNMGLRETGRIGKIIVHPTNPD
                     IVFACALGRSTGPQQERGVYRTGDGGKTWSRVLFVNENTGCSGIAIDAKNPDVLYAGT
                     WEMVMHTWAMFSGGPGSGVYKSTDGGLTWQKLTHEGLPKSPVGKIDVAVAPSNSNRVY
                     ALIQTANQGSLWRSDDAGATWAVSSWDRRLIGRAGYYIRIAVNPANENEVLVANSSFL
                     RSMDGGKTFPIAQGGGCGDCHDIWMDPKNPNHWIATGDAGAGITRNHGRAFTQIALPI
                     AQMYHVAIDDQAPYWVYGNRQDNGTMRGPSTAPEATTSTRGIAAGAGRGRGGGRGAAD
                     S"
BASE COUNT          242 a          563 c          565 g          283 t
ORIGIN      
        1 gaatcagccg cgccgcgtcc acccccgcga ccacgcccgg cgccggcggc aatgccgcgc
       61 gtcgacgtcg tcgcttcggg cgcggtgctc ggcccgcgca tcgtgccgtt gtcctggcgg
      121 ttgccataca cccagtacgg cgcctggtcg tcaatcgcga cgtgatacat ctgcgcgatg
      181 gggagcgcga tctgcgtgaa cgcgcggccg tggttgcgcg tgatgcccgc tccggcgtcg
      241 ccggtggcga tccagtgatt cggattcttc ggatccatcc agatgtcgtg gcagtcgccg
      301 cacccgccgc cctgcgcgat cgggaacgtc ttgccgccgt ccattgaccg caggaacgag
      361 ctgttcgcta cgaggacttc gttctcgttc gccgggttca ccgcgatgcg gatgtagtag
      421 cccgctcgac cgatgaggcg ccgatcccag ctggacacgg cccacgtcgc gcccgcgtca
      481 tccgaacgcc acagcgagcc ctgattcgcc gtctggatca acgcatacac gcggttgctg
      541 ttggacggcg cgaccgcgac gtcaatcttg cccaccggag acttgggcaa tccttcatgc
      601 gtgagctttt gccacgtgag gccgccgtcg gtggacttgt agacgccgct gccgggtcca
      661 ccgctgaaca tcgcccacgt gtgcatcacc atttcccagg tgccggcgta gagcacgtcg
      721 gggttcttgg catcaatcgc gatgcctgag cagccggtgt tctcgttgac gaacagcact
      781 cggctccacg tcttgccgcc gtcgcccgtg cggtagacgc cgcgttcctg ctgcggaccc
      841 gtcgagcggc ccagcgcgca cgcgaacacg atgtccgggt tggtggggtg cacgatgatc
      901 ttgccgatgc ggcccgtctc acgcaggccc atgttgaccc acgtcttgcc cgcgtcgctg
      961 gacttgtaga tgccatcgcc catcacgtcg gcgtcgcgga tggcccacgc ttctcccgtg
     1021 ccggcccaca cgatctgcgg cgcactcggc gacaccgcca gcgcgccgat ggcctggact
     1081 ggcatgctgt cggacaccgg gacggacgtg ttgccgccgt cggtggtctt ccagacgccg
     1141 cccgacgccg cgccgaagta gtaagtcatc gtgtcgccgg gtattccggc cgccgcggag
     1201 atgcgtccgc cgctggaggg cccgagcagc tggaagcggg ggcgctcggg cgcgcggtca
     1261 ccgccgccgg gcccgcgctg cgcggcagcg gacgacgaaa caacggcggc tgcaaggatg
     1321 acgaccggga ggcaacggga gcgacggttc atggcgggga ttcggcgggg gccgggttga
     1381 cgacgaggtt ctccatctac tctatcttct ggtgcggcga cggccagcgg tggccctcgg
     1441 caccgacttc agtgcgcgat acaaaatggc acttcgagcc gcggtgtgtg ggttggtcgt
     1501 gatggcgctg accggatgct acaggtatgt gccgacgccc atcgggaacg tcaacccggg
     1561 tgaaagcgta cgtctcgcga tcgctgacac gagcatccgt gccacggacg gcgggccgga
     1621 gccggaactg gccaggcgcc ttggcgccgg gac
//
LOCUS       DASVOR010000249         8250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410789,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000249 DASVOR010000000
VERSION     DASVOR010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410789"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            615..1931
                     /locus_tag="VF516_05370"
     CDS             615..1931
                     /locus_tag="VF516_05370"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8107136.1"
                     /translation="MTTTAVGGLLAGCASQSVEEPAPATTSTQQMVVGDQLAGISTTD
                     FAAAKANFMQAEGVNDGLGPIFNERACGNCHTQGAIGGAGVQIERRFGKFVNGIFNTL
                     SNEGGSLRQLMTLGTFTNVNGQTCTVPLEHEPADATVHNVGRLTTPLFGLGLVDSLPD
                     SAFDSVVANEPAAVRGTANRVRVLLPDPHDPSQSINSTRVGRFGWKAGIANLVQFAAD
                     AYLNEMGITTQHCFKGTSVLSFATESKPNGVAQPAGCDDNGPGGPGIPAGTDDGVGSC
                     AGGLTEIQEDVDNFNKFMTFLAPPPPDSTIDPTVAQNAFNLGVQIGCTGCHFPGPYTT
                     PAAPANGVPGNMQFFPGSDFALHDMGSLGDLIGNDGDTVARTRMMRTAPLWGLRFRTK
                     FLHDGRTSDLTTAILAHDGQALAARNAFNALSAADKSTLLTGLKAL"
     gene            complement(1962..2597)
                     /locus_tag="VF516_05375"
     CDS             complement(1962..2597)
                     /locus_tag="VF516_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8107137.1"
                     /translation="MTPIIYGTEFSTYVRSVRMAFEEKGAEYKLHDVSVLKGEQKQAA
                     HLSRNPFGTVPAFEHDGLEVYETSPILRYIDQVWPAPALTPEDPKQRARMNQIISIID
                     YHGYASMIGQIVVQRLFTALLANGTDENIVQAGIPRAKLCLQEFERIKGHHTFLAGDR
                     VSLADLYLVPIIFYLKLTPEGELLASHKSLAAWWEAMSDRKSVKATAPNLG"
     gene            3011..3637
                     /locus_tag="VF516_05380"
     CDS             3011..3637
                     /locus_tag="VF516_05380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEX8107138.1"
                     /translation="MSEATLHNLREARAIAILRANDHALARDALAAAVRGGFRVLEVT
                     LSTPGAIEIIADLSRDKQLLVGAGTVLTVEQARAAVHAGARFLVSPVIDTEVVVEASR
                     LGAVAIPGCGTATELWTAHRAGAPLQKLFPAPAGGPAWLRAVLAPMPFLRLIPTNGVD
                     ADNASEWLAAGAFAVGCVRSLFPPEELAARAWDSIERRARALLAAVLD"
     gene            complement(3650..4900)
                     /locus_tag="VF516_05385"
     CDS             complement(3650..4900)
                     /locus_tag="VF516_05385"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEX8107139.1"
                     /translation="MVDGTTEPGWQPLIVDPERRAAIAGVIAEIVAAIDSWRRDRPTT
                     SDDDADYATLRIYTATDDTVPDPDDATGQALASAITKLGDNHAPGLYGGAARVAFAVG
                     HLSAGEDADLACEMIERSLLRFLGQPTASYDLISGLVGIAVPVLQRIADGKPSESSEP
                     LARNILDQLERLARPMDHGLAWHTPPELLPHWQRLRAPEGYTNLGLAHGIPGVVAILA
                     RYLTAGVDAPRARVLLEGGVDYLRSVAAPTAGNRYAAWLPAQEGGTCRVAWCYGDLGV
                     AVALLSAAAATGRDDWRRDALELAHGMAARPFESSHVVDAGLCHGAAGVAHLFNRLAQ
                     ATGDTAFARTADTWFGHTLAIRRPDGLAGFPRALYNDGNQIFEPSADLINGATGVALA
                     LHAAISPIEPAWDQLMLADLSPVT"
     gene            4990..7446
                     /locus_tag="VF516_05390"
     CDS             4990..7446
                     /locus_tag="VF516_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8107140.1"
                     /translation="MTVPSQARVVVIGGGVIGCSVAYHLAHLGWTDVVLLERDRLTSG
                     TTWHAAGLIVTFGSTSETSTEMRKYTRDLYARLEAETGQSTGLRQCGFIEVAADPDRL
                     EEYRRVAAFNRYCGVDVHEISPRQVAELFPLAQVDDVLAGFYVAGDGRADPVGVTMAL
                     AKGARQLGVRILEGVPCTGVTRRRGAVTGVTTPAGPIACEYVVNCAGMWARQLGAAAG
                     VTIPLQAAEHYYLITERIPELSASWPVLEDPASYGYFREEVGGLMIGLFEPVCAPWQV
                     GGVPDDFSFGEIQPDWDRMAGYIETAMRRVPIAMDVGVRKLFCGPESFTPDLQPCVGE
                     APELKNYFVAAGLNSIGILTGGGLGRVLAHWIVTGRPDVDVTGFHIDRLQPYQATPDY
                     RRTRTVESLGMVYKCHYPSYVMQTARGARRSPIHDRLAARGALFRDVSGWEGADWYAP
                     PGVEPRVELSWGRPSWFAYWAAEHRAARDGVIAMDMSFMSKFLVEGRDAGRLLDWISA
                     NRVDGPAGVITYTQWLDAAGKLQADLTVAKLDDEQFWVVASDTAHRHALTWLRRHVAD
                     HHAFVADLTAAYGQLNLQGPRSRELLQALTSADVSDAALPFRGVRDIDIGFAQVRCIR
                     ITYLGELGYELYIPAEQMVHVYDRIVAAGERVSLVHAGLKALASLRMEKAYRDYGHDI
                     DNTDTVLEAGLGFAVDLHKPGGFLGKDAVLAQKAAGPLHKRLVQVLVTDPEPLLFHAE
                     VVRCDDQPCGYIRAASYGHTLGGAVGLAMIEADRVPGGMIDQRALDAARWDVEIAGTR
                     YPARVSLRPLYDPENRRTKG"
     gene            7713..8024
                     /locus_tag="VF516_05395"
     CDS             7713..8024
                     /locus_tag="VF516_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107141.1"
                     /translation="MKRHRRPRPKVPKQQLALNLDPEILTTISLVYDGLVEVEALAIT
                     ADEAVTNLPVVPTGKYKRTLKRLYTLVSKTSERAQGVLERGEELTAQIAAARAGKRPA
                     R"
BASE COUNT         1224 a         2858 c         2879 g         1289 t
ORIGIN      
        1 tgggagagtt cgatcgatgc ccggccgatg accggcgatg gggttgcgga gccatccttc
       61 ggcggcagct cgtgacgcac attttgcttt gatatcctcg acggatgtcc ctcttacgct
      121 gaccaagaac gtaatgttga caacatcaat aacttggtct gttgagggtg cggcttcgct
      181 gagaccgcgt catgatggcc gctccgccgt cgcgagtacg tgacatctcg tacatcgcag
      241 tttttcgata ctcaggattc ctgtatatcc aattcatcct cagattcttg tcggttgggg
      301 cgaatttgtg tctgtctcca tactgctgag cttcggttaa ctcgaattgt actcgtgacg
      361 ccgaaaaacc agattgacat gtgcggacac acagccgtag tgttcgtgcc gtcgaacggg
      421 tgaaccatca ataaactggt atcccgattg gcagtcgtca gcggtcttcc ctgatcgtgc
      481 ggggcgcatc tcgccatgat cgtcggggga tcgttcgagt tggctcacga catggtgcgt
      541 cgtgcgcagt gtccatcttg tagccggaga aacatgaaat gaatcaaatg acatggatcc
      601 gcagccgagt gctcgtgaca accaccgcag tcggcggctt gctcgctgga tgcgcgagcc
      661 agtcggtcga ggagccggcg ccggccacca cgagcacgca gcagatggtg gtcggcgatc
      721 agctcgccgg gatctcgacg acggacttcg cggccgccaa ggccaatttc atgcaggcgg
      781 aaggcgtcaa cgacggcctg ggcccgatct tcaacgagcg cgcgtgcggc aactgccaca
      841 cgcagggcgc cattggcggg gccggcgtgc agatcgagcg ccggttcggc aagttcgtca
      901 acggcatctt caacacgctg tcgaacgagg gcggctcgct gcgccagctg atgacgctgg
      961 gcacgttcac caacgtgaac ggccagacct gcaccgtgcc gctcgagcac gagccggccg
     1021 atgccacggt gcacaacgtc ggccgcctga ccacgccgct gttcggcctc ggcctggtcg
     1081 actcgctgcc cgactcggca ttcgacagcg tggtcgccaa cgagcccgcc gcggtgcgcg
     1141 gcacggccaa ccgcgtcagg gtcctgctgc cggatccgca cgatccgagc cagtcgatca
     1201 actcgacgcg ggtcggccgg ttcggctgga aggcgggcat cgcgaacctg gtgcagttcg
     1261 cggccgacgc ctatctcaac gagatgggca tcacgacgca gcactgcttc aagggcacgt
     1321 cggtcctgtc gtttgcgacc gagtccaagc ccaacggagt ggcgcagccg gcgggctgcg
     1381 atgacaacgg gcccggcggg ccgggcatcc cggcgggcac cgacgacggc gtcggcagct
     1441 gcgcgggcgg cctgaccgag atccaggagg atgtcgacaa tttcaacaag ttcatgacgt
     1501 tcctcgcgcc gccgccgccc gactcgacga tcgatcccac ggtcgcgcag aacgcgttca
     1561 accttggcgt gcagatcggc tgcaccggct gccacttccc cggcccgtac acgacgccgg
     1621 ccgcgccggc caacggcgtc cccggcaaca tgcagttctt cccgggctcg gacttcgcgc
     1681 tgcacgacat gggctcgctc ggcgacttga tcggcaacga cggcgacacg gtcgcgcgga
     1741 cccggatgat gcggaccgcg ccgctgtggg gcctgcggtt ccgcaccaag ttcctgcacg
     1801 acggccgcac gtcggatctc accacggcga tcctcgcgca cgacggccag gccctggcgg
     1861 cgcgcaacgc gttcaacgcg ctgagcgcgg cggacaagag cacgctgctc accggcctca
     1921 aggcgctgtg accggcgcgc cgggggtgag cccggcggtg gtcagccgag gttcggcgcc
     1981 gtcgccttga cgctcttgcg atcgctcatc gcctcccacc acgcggccag gctcttgtgg
     2041 gaggcgagca gctcgccctc gggggtgagc ttgaggtaga agatgatcgg aaccaggtag
     2101 aggtcggcga ggctgacgcg gtctccggcg aggaacgtgt ggtggccctt gatgcgctcg
     2161 aactcctgca ggcacagctt ggcccgcggg atgccggcct ggacgatgtt ctcgtcggtg
     2221 ccgttggcga gcagcgccgt gaacaggcgc tgcacgacga tctggccgat catcgacgcg
     2281 tacccgtgat agtcgatgat actgatgatc tgattcattc gcgcgcgctg cttcggatcc
     2341 tcgggagtca acgcaggcgc cggccacacc tggtcgatgt agcgcaggat cggtgaggtc
     2401 tcgtacacct ccaggccgtc gtgctcgaac gccggcaccg tgccgaaggg attgcggctc
     2461 agatgcgcgg cttgcttttg ctcgcccttg agcacgctga cgtcatgcag cttgtactcg
     2521 gcaccctttt cctcgaaggc cattcgcacg ctgcggacgt aggtactgaa ctcggtgcca
     2581 tagatgatgg gcgtcatgcg ggtcctccgt tgcggtgagc tggacgtggc cggcgccacg
     2641 cgcgcgcgag gatacgctcg aaccgcggcg cgcgacgtcc ggagcgaggc tcacgcgcac
     2701 gggtacgctc gggtactccg cacgtggatc cacgtgcgcc aataggaacc taactgctcg
     2761 tatgtatgaa gttattccgg ctgcatgcga cagaacaccg cgtggcgcgg ccgagggtcg
     2821 gcggccatct tccggccgcc atgtcgacca tcagccgcat tccagagatc gtcctccgcc
     2881 agcgcgcgat cgcgcgctca ctgccagcgt tcgggctcgc gctcgcgggc gccgccgtcg
     2941 ggttgctcat gctcgctgtc cgcgcctgaa cggctcgact ggtgccggct ccagtcgtac
     3001 gctgcggggc atgtcagagg cgacgctcca caacctccgc gaggcgcgcg cgatcgcgat
     3061 cctccgggcc aacgaccacg cgctcgcgcg cgatgcactc gccgcggcgg tgcgcggcgg
     3121 atttcgcgtc ctggaggtca cgctgtcgac ccccggggcg atcgagatca tcgcggacct
     3181 ctcgcgcgac aagcagctgc tggtcggcgc cgggacggtg ctcaccgtcg agcaggcgcg
     3241 cgccgcggtc cacgccgggg cgcggttcct ggtctcgccg gtgatcgaca ccgaggtcgt
     3301 ggtcgaggcg tcgcggctcg gcgccgtcgc gatcccgggc tgcggcaccg ccaccgagct
     3361 gtggaccgcc caccgtgccg gcgcgccgct ccagaagctg ttcccggctc ccgccggcgg
     3421 cccggcgtgg ctgcgcgcgg tgctggcgcc gatgccgttc ctgcgcctga tcccgaccaa
     3481 cggggtcgac gccgacaacg cctccgagtg gctggccgcc ggagcgttcg ctgtcggctg
     3541 tgtgcgctcg ctgttcccgc ccgaggagct cgccgcgcgg gcctgggact cgatcgagcg
     3601 ccgcgcccgc gcgctgctcg ccgccgtgct cgactagcgt cgcgagcggt caggtcaccg
     3661 gcgacaggtc ggcgagcatc agctgatccc acgccggctc gatcggcgag atcgccgcgt
     3721 gcagcgcgag cgcgacgccg gtcgcgccgt tgatcaggtc ggccgacggc tcgaagatct
     3781 ggttgccgtc gttgtacagc gcgcgcggga acccggcgag gccgtccggg cgccggatcg
     3841 cgagcgtgtg gccgaaccag gtgtcggcgg tcctggcgaa cgcggtgtcg ccggtggcct
     3901 gggcgagccg gttgaacagg tgagcgacgc cagcggcgcc gtggcacagc ccggcgtcga
     3961 cgacgtgcga cgactcgaac gggcgggccg ccatgccgtg ggcgagctcg agcgcgtcgc
     4021 ggcgccagtc gtcgcggccg gtcgccgcgg cggcggatag cagcgcgacc gcgacaccga
     4081 ggtcgccgta gcaccaggcg acccggcagg tcccgccctc ctgcgccggc agccacgccg
     4141 cgtagcggtt gccggcggtc ggcgccgcga ccgagcgcag gtagtcgacc ccgccctcga
     4201 gcagcacgcg cgcccgcggg gcgtcgaccc cggcggtgag gtagcgcgcg aggatcgcga
     4261 ccacgcccgg gatgccgtgc gccaggccga ggttggtgta gccctcgggc gcccgcaggc
     4321 gctgccagtg tgggagcagc tcgggcgggg tgtgccaggc gaggccgtgg tccatcggcc
     4381 gggcgaggcg ctcgagctga tcgaggatgt tgcgcgcgag cggctcgctc gactccgacg
     4441 gcttgccgtc ggcgatccgc tgcagcaccg gcacggcgat cccgaccagc ccgctgatca
     4501 ggtcgtagct cgcggtgggc tggccgagga accgcagcag cgagcgctcg atcatctcgc
     4561 acgccaggtc ggcgtcctcg ccggcgctca ggtgaccgac cgcgaacgcg acgcgggccg
     4621 cgccgccgta cagccccggc gcgtggttgt cgccgagctt ggtgatcgcc gacgccagcg
     4681 cctggccggt cgcgtcgtcc ggatcgggca ccgtatcgtc ggtcgcggtg tagatccgca
     4741 gggtcgcgta gtcggcgtcg tcatcgctgg tcgtcgggcg gtcgcgccgc cacgaatcga
     4801 tcgccgcgac gatctcggcg atcacccccg cgatcgcggc gcggcgctcg gggtcgacga
     4861 tcaggggttg ccagccaggc tcggtcgtgc cgtccaccac ctggctagcc taccaggccc
     4921 cggcgccggc tggccggtat gattcggccg gcggtcgagc cagcgggatc gcgttattct
     4981 gtgtggccca tgaccgtacc ctcgcaggcg cgcgtcgtcg tgatcggcgg cggcgtgatc
     5041 ggatgctcgg tggcctacca cctcgcccac ctgggctgga ccgacgtggt gctgctcgag
     5101 cgcgatcgcc tgacctcggg caccacgtgg cacgcagccg gcctgatcgt gacgttcggc
     5161 tcgacctcgg agacctcgac ggagatgcgc aagtacaccc gcgatctcta cgcgcggctc
     5221 gaggccgaga ccggccagag cacgggcctc cggcagtgcg gcttcatcga ggtcgccgcc
     5281 gacccggatc gcctcgagga gtaccgccgg gtcgccgcgt tcaaccgcta ctgcggggtc
     5341 gacgtccacg agatctcgcc gcgccaggtc gccgagctgt tcccgctcgc gcaggtcgac
     5401 gacgtgctcg ccgggttcta cgtcgccggc gacggccgcg ccgatccggt cggggtcacg
     5461 atggcgctcg ccaagggcgc ccgccagctc ggcgtgcgga tcctcgaggg cgtgccgtgc
     5521 accggggtga cccggcggcg cggcgcggtg accggggtga ccacgccggc cgggccgatc
     5581 gcctgcgagt acgtcgtcaa ctgcgccggg atgtgggcgc gccagctcgg cgcggcggcc
     5641 ggcgtgacca tcccgctcca ggccgccgag cactactacc tgatcaccga gcggatcccc
     5701 gagctgtcgg cgagctggcc ggtgctcgag gacccggcgt catacggcta cttccgcgag
     5761 gaggtcggcg gcctcatgat cggcctgttc gagccggtgt gcgcgccgtg gcaggtcggc
     5821 ggcgtgcccg acgacttctc gttcggcgag atccagcccg actgggaccg gatggcgggc
     5881 tacatcgaga ccgcgatgcg gcgggtgccg atcgcgatgg acgtcggcgt ccgcaagctg
     5941 ttctgcggtc ccgagagctt cacgccggac ctccagccgt gcgtcggcga ggcccccgag
     6001 ctcaagaact acttcgtcgc cgccgggctc aactcgatcg gcatcctgac cggcggcggc
     6061 ctgggccgcg tgctcgcgca ctggatcgtg accggccggc ccgacgtcga cgtcaccggc
     6121 ttccacatcg accggctgca gccctaccag gcgacgccgg actatcgccg gacccgcacc
     6181 gtcgagtcgc tcggcatggt ctacaagtgc cactacccga gctacgtgat gcagaccgcg
     6241 cgcggcgccc ggcgctcgcc gatccacgac cggctcgccg cgcgcggcgc gctgttccgc
     6301 gacgtcagcg gctgggaggg agccgactgg tacgccccgc cgggtgtcga gccgcgcgtc
     6361 gagctgtcgt ggggccggcc gagctggttt gcgtactggg ccgccgagca ccgcgcggcg
     6421 cgcgatggcg tgatcgcgat ggacatgtcg ttcatgtcca agttcctcgt cgagggccgc
     6481 gacgccgggc ggctgctcga ctggatcagc gccaaccggg tcgacggccc ggccggcgtc
     6541 atcacctaca cccagtggct cgacgccgcc ggcaagctgc aggccgacct gaccgtggcc
     6601 aagctcgacg acgagcagtt ctgggtggtg gcgtccgaca ccgcccaccg ccacgcgctg
     6661 acctggctgc gccgccacgt cgccgatcac cacgcgttcg tcgccgacct caccgccgcc
     6721 tacggccagc tcaacctgca gggcccccgg tcgcgcgagc tgctccaggc gctgaccagc
     6781 gccgatgtgt ccgacgcggc gctgccgttc cgcggcgtgc gcgacatcga catcgggttc
     6841 gcgcaggtcc gctgcatccg gatcacctac ctcggcgagc tcggctacga gctgtacatc
     6901 cccgccgagc agatggtgca cgtctacgac cggatcgtcg ctgccggcga gcgtgtcagc
     6961 ctggtccacg ccgggctcaa ggcgctggcc agcctgcgga tggagaaggc ctaccgcgac
     7021 tacgggcacg acatcgacaa caccgacacc gtgctcgagg ccggcctggg cttcgcggtc
     7081 gatctccaca agccgggcgg cttcctcggc aaggacgccg tgctggccca gaaggcggcc
     7141 ggcccgctcc acaagcggct ggtccaggtg ctggtcaccg accccgagcc gctgctgttc
     7201 cacgccgagg tcgtgcgctg cgatgaccag ccctgcggtt acatccgcgc cgcgtcgtac
     7261 ggccacacgc tcggcggcgc cgtcggtttg gcgatgatcg aggccgatcg cgtccccggc
     7321 ggcatgatcg accagcgcgc cctcgatgct gcgcgctggg acgtcgagat cgccggcacg
     7381 cggtacccgg cgcgggtgtc gctgcgcccg ctgtacgacc cggagaaccg gcggaccaag
     7441 ggatagagca ccgacctcga tccgccggcg aggccagttg tcggttgtcg gttgtcggtt
     7501 gtcggttgtc ggctttgcac cttttctcgt cggaaaccgc ttcctgcacc tttttgattt
     7561 cggctgcacc tttagtcagt gacgcgccgt tctccgtcga ggattgagcg acgcgtcatg
     7621 cgacgtcgga tgtcgtagcc gcttcgtaac gtgggcgcga acccatagct ggcccgacgg
     7681 tgttgaagca ccggcggacc aaggagccca cgatgaagcg tcatcgcaga ccccgaccga
     7741 aggtaccgaa gcagcaactc gccctcaacc tggaccccga gatcctgacc acgatctcgc
     7801 tggtctacga tggcctcgtc gaggtcgagg cgctggccat caccgccgac gaggcggtca
     7861 cgaatctgcc ggtggtcccg accggcaagt acaagcggac cctgaagcgg ctgtacacgc
     7921 tcgtgtccaa gacctccgag cgcgcacagg gcgtgctgga gcggggcgag gagttgaccg
     7981 cgcagatcgc agcggctcgc gctggcaagc gcccggcacg ctgacgtcag cgccacccgt
     8041 cgaccatgca agcatcgggg gcgatccgat gcttgcattc cggagtggtc gcatgcaagc
     8101 atcagatcca ggagatgctt gcatgacaga cgacaagaag atcacgggca aggccaaggg
     8161 aggcatcgcg cgcaaagaag cgctgtcgcc tgaggagcgc aggagcatcg cggtcaaggc
     8221 ggccgaggca cgctgggggg tgaagccact
//
LOCUS       DASVOR010000250         3046 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382201,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000250 DASVOR010000000
VERSION     DASVOR010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382201"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VF516_05400"
     CDS             complement(<1..917)
                     /locus_tag="VF516_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX8107142.1"
                     /translation="MRGALIHGVLLAVMLVYGYRTWTRDTTIQPDFGSVVLWDRSEND
                     LVSLEFKTEKMINKIERRGQGKDAYWWGVETTIEKKPKPAAAPKPGEGSAAGSDAGSG
                     SSAGSGSSAGSGSNAGSGSAAPAVAEEEIRKNREYPLGEPGEKLVKDFAAARALRDLG
                     VPNDEQKKDYKLNDAKSVLVVTFKDGSRSFLVGGTVYGSSDRYVADQKTNHAYVLSKD
                     LISNLEIGQSTLHLLDPHGFDSQKVEEVTIEAGGKSKTVARVKTGVEGQQVKTWGDPQ
                     TKKADQTIANFVDNTNNLRPSEFLPDLKM"
     gene            complement(914..2875)
                     /locus_tag="VF516_05405"
     CDS             complement(914..2875)
                     /locus_tag="VF516_05405"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX8107143.1"
                     /translation="MRTASPWWASLAFGAGLLLFFLGERLFGHLSGARFVLTGLGVLL
                     ILGVTAARAWTMSGSSGARRRVERTLLLCHLGTVLALVLYVLTTDWGLAKLNLTDTAA
                     THFHGAVTVLYLAVLTASIVPLLIAELSLGIALRTAFDVQRAAGDEAAGVEYFRVREL
                     GWSGLSVGLALSFLMVTCQVADQRNVQRDVSYFKTSSPGDSTVKMVASSSDPLRVLLF
                     FPDANEVKSQVVDYFQALAQASGRLSVEVHDRLADAELAGKYKVVKDGTVVLLRGTGD
                     KEKSQTIDIDPDITRARTSSSKLRNLDREVNSAMLKVLRDKRKAYLVVGHGEINDPDS
                     VPAEIKARVGQHATTQFRKRLADLNYEIKNLSLIDLAKDVPDDATVVVLLSPAVPLQP
                     AEWASFGRYLERGGHMLIALDPNGFTSMGLLEGKLAIKMLPGHLTDEEKFVPLRRTLS
                     DRRAVLTTQFSAHASTTTLSRNPDHPVIFNDAGALDDAPYTGKPEQAPKKTFTIHSQD
                     SSFLDLNDNFAFDAATEKKQRWNLAAAIEGPKDGTKDGYRAAVFSDVDLFADILARDP
                     LGRPVMLMLADAAGGGVVEDIVHWLGGEEVFSGEIVSEDDKPIQHTKNQDVVWFMLTI
                     IGFPLLVLTLGLLGTTRRRRGSKKAEVTL"
BASE COUNT          445 a         1045 c         1014 g          542 t
ORIGIN      
        1 tccatcttga ggtcggggag gaactcgctg ggccgcaggt tgttggtgtt gtcgacgaag
       61 ttcgcgatcg tctggtcggc cttcttggtc tgcggatccc cccaggtctt gacctgctgg
      121 ccctcgacgc cggtcttgac ccgcgcgacg gtcttggact tgccacccgc ctcgatggtc
      181 acctcctcga ccttctggga atcgaagccg tgcgggtcga gcaggtgcag cgtcgactgg
      241 ccgatctcca ggttggagat caggtccttc gacagcacgt aggcgtggtt ggtcttctga
      301 tcggcgacgt agcgatcgct gctgccatag accgtgccgc cgaccaggaa gctgcgcgat
      361 ccgtccttga acgtcacgac caggacgctc ttggcgtcgt tcagcttgta gtccttcttc
      421 tgctcgtcgt tcggcacgcc gagatcgcgc agcgcccggg ccgcggcgaa gtccttgacc
      481 agcttctcgc cgggctcgcc cagcgggtac tcgcggttct tgcggatctc ctcctcggcg
      541 acggcggggg ccgcgctgcc gctgcccgcg ttgctgccgc tccccgcact gctgccgctc
      601 ccggcgctgc tgccgctgcc tgcgtcgctg ccggccgcgc tgccctcgcc gggcttgggc
      661 gccgccgccg gcttgggctt cttctcgatc gtcgtctcga cgccccacca gtaggcgtcc
      721 ttgccctggc cgcggcgctc gatcttgttg atcatcttct cggtcttgaa ctcgagcgac
      781 accagatcgt tctcggaccg gtcccacagc acgaccgagc cgaagtccgg ctggatcgtc
      841 gtgtcgcggg tccacgtccg gtatccgtac acgagcatca cggcgagcag gacgccgtgg
      901 atcagtgcgc ctctcatagc gtcacctcgg ccttcttgga gcctcggcgc cggcgggtgg
      961 tcccgagcag gccgagcgtc agtacgagca aggggaatcc gatgatcgtc agcatgaacc
     1021 acacgacgtc ctggttcttg gtgtgctgga tcggcttgtc gtcctccgag acgatctcgc
     1081 ccgagaacac ctcctcgccg ccgagccagt ggacgatgtc ctcgaccacc ccgccgcccg
     1141 cggcgtcggc cagcatcagc atcaccgggc ggccgagcgg gtcgcgggcg aggatgtcgg
     1201 cgaacagatc gacgtcggag aacaccgccg cgcggtagcc gtccttggtg ccgtccttgg
     1261 gcccctcgat cgccgcggcg aggttccagc gctgcttctt ctcggtcgcg gcgtcgaacg
     1321 cgaagttgtc gttgaggtcg aggaagctcg agtcctgcga gtggatcgtg aacgtcttct
     1381 tcggcgcctg ctccggcttg ccggtgtacg gcgcgtcgtc gagcgcaccg gcgtcgttga
     1441 agatcaccgg gtggtcgggg ttgcgcgaca gcgtcgtggt cgaggcgtgg gccgagaact
     1501 gggtggtcag caccgcgcgg cggtcgctca gcgtccggcg cagcggcacg aacttctcct
     1561 cgtcggtcag gtggccgggc agcatcttga tcgccagctt gccctcgagc aggcccatcg
     1621 aggtgaagcc gttgggatcg agcgcgatca gcatgtgccc gcctcgttcg aggtagcgcc
     1681 cgaacgaggc ccactccgcc ggctgcagcg gcaccgccgg cgacagcaag acgacgaccg
     1741 tcgcgtcgtc cggcacgtcc ttggccaggt cgatcaagct gaggttcttg atctcgtagt
     1801 tgaggtcggc caggcgcttc ctgaactgcg tggtcgcgtg ctggccgacc cgggccttga
     1861 tctccgccgg caccgagtcc gggtcgttga tctcgccgtg gccgaccacc aggtacgcct
     1921 tgcgcttgtc gcgcagcacc ttgagcatcg ccgagttgac ctcgcggtcg aggttgcgca
     1981 gcttgctgct cgaggtccgc gcccgcgtga tgtcgggatc gatgtcgatg gtctgggact
     2041 tctccttgtc gccggtgccg cgcaacagca ccacggtgcc gtccttgacc accttgtact
     2101 tgcccgcgag ctcggcgtcg gccaggcggt cgtggacctc gaccgagagc cggccgctcg
     2161 cttgcgccag cgcctggaag tagtccacga cctgcgactt gacctcgttg gcgtcgggga
     2221 agaacagcag cacgcgcagc ggatcgctcg agctcgcgac catcttcacg gtcgagtccc
     2281 cgggcgacga cgtcttgaag tagctgacgt cgcgctgcac gttgcgctga tcggcgacct
     2341 ggcaggtcac catcaggaac gacagcgcga ggccgaccga cagccccgac cagccgagct
     2401 cgcggacccg gaagtactcg accccggcag cctcgtcccc ggcggcgcgc tggacgtcga
     2461 acgcggtgcg cagcgcgatc ccgagcgaca gctcggcgat cagcagcggc acgatcgacg
     2521 cggtcagcac cgcgaggtac agcacggtga ccgcgccgtg gaagtgggtc gccgcggtgt
     2581 cggtcaggtt gagcttggcc agcccccagt cggtggtcag cacgtagagc accagcgcca
     2641 gcacggtgcc gaggtggcag agcagcagcg tgcgctcgac ccggcgccgc gctccgctcg
     2701 agcccgacat cgtccacgcg cgcgccgcgg tgaccccgag gatcagcagc acgcccaggc
     2761 cggtcagcac gaaccgcgcg cccgacaggt ggccgaacag ccgctcaccc aggaagaaca
     2821 acagcaggcc tgctccgaag gcgagcgagg cccaccacgg agacgcagtt ctcattgcca
     2881 tcgcttcgcc tccagcgtct tgatcgacag cagcaggaag aagtaggtcg ccgctacgta
     2941 gtagatgacg tccttgaggt tcaggattcc cttcatgaat ccgtactggt agtggaccca
     3001 ccacaggtcg atctgctcca gcacgtcctt gagcggcggg tcgacc
//
LOCUS       DASVOR010000251         1734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_528322,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000251 DASVOR010000000
VERSION     DASVOR010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528322"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VF516_05410"
     CDS             <1..661
                     /locus_tag="VF516_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8107144.1"
                     /translation="LAPKGQSATGQDQDDTVMMPWTTAMKRIVGKDQTWLDDILCSAR
                     STDAIAPATAQIQELLRQRHHIEPGAEDDFNIRHPEELLQARIKTSKTLRLLLLGLAG
                     IAMVVGGIGVMNVMLASVSQRVTEIGLRCAIGARPAAIQLQFLGEAVALSLLGGALGV
                     GLGELSGYIFEDKLGWRLAMSPEISAIAVGVSIVAGVVFGLYPAVRASRVDPIVALRT
                     E"
     gene            complement(666..1370)
                     /locus_tag="VF516_05415"
     CDS             complement(666..1370)
                     /locus_tag="VF516_05415"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX8107145.1"
                     /translation="MSIQTITLTLLLLLAGSLAAAPPAHADRSPILKALLHNDGEGFT
                     PPAENKALQALLAQHPALTFFEACGVGDTAEIARQLKRDPKLATAWTEFGASALHLAA
                     FSGVPAAVQLLLDRGAEINARARSKFKNTPLQAALLAGQFATAKLLLERGADPLVRQA
                     LGFTPLQEAALLGRRDLVDLFLGAGAEINSRTDDGRTAVTEALRGKHPELADYLRSKG
                     GHGAEITADLMAPPKE"
     gene            1481..>1734
                     /locus_tag="VF516_05420"
     CDS             1481..>1734
                     /locus_tag="VF516_05420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107146.1"
                     /translation="MTVRVRPHPSLLLAALAAWLAVSLPTWLTAQASPRFPLWAIAWA
                     VCGAAMVAASLRPRLDAIAAVALAVQGAAVATMVGLLCNG"
BASE COUNT          212 a          591 c          673 g          258 t
ORIGIN      
        1 gctcgccccc aagggccagt cggccaccgg gcaggaccag gacgacaccg tgatgatgcc
       61 gtggaccacc gcgatgaagc ggatcgtcgg caaggatcag acctggctcg acgacatcct
      121 gtgctcggcg cgctcgaccg acgcgatcgc gccggcgacc gcccagatcc aggagctgct
      181 gcgccagcgc catcacatcg agcccggcgc cgaggacgac ttcaacatcc gccaccccga
      241 ggagctgctc caggcccgga tcaagaccag caagacgctg cgcctgctgc tgctcggcct
      301 ggccgggatc gcgatggtcg tcggcgggat cggcgtgatg aacgtgatgc tggcgtcggt
      361 ctcgcagcgg gtcaccgaga tcggcctgcg ctgcgcgatc ggcgcccggc cggcggcgat
      421 ccagctccag ttcctcggcg aggccgtcgc gctgtcgctg ctcggcggcg cgctcggcgt
      481 cgggctcggc gagctcagcg gctacatctt cgaggacaag ctggggtggc ggctcgcgat
      541 gtcgccggag atcagcgcga tcgcggtggg cgtgtcgatc gtcgcgggcg tcgtgttcgg
      601 gctgtacccc gcggtgcgcg cgtcgcgggt cgatccgatc gtcgcgctgc gcaccgagta
      661 gcgggtcact ccttgggcgg ggccatgagg tccgcggtga tctccgcgcc gtgcccgccc
      721 ttgcttcgca ggtagtcggc gagctcgggg tgcttgccgc gcagcgcctc ggtcaccgcg
      781 gtccggccgt cgtcggtgcg cgagttgatc tcggcgccgg cgccgaggaa caggtcgacc
      841 aggtcgcggc ggccgagcag cgcggcctcc tggagcggcg tgaagcccag ggcctggcgg
      901 accagcggat ctgcgccgcg ctcgagcagc agcttggcgg tcgcgaactg gccggcgagc
      961 agcgcggcct gcagcggggt gttcttgaac ttgctgcgcg cgcgggcgtt gatctcggcg
     1021 ccgcgatcga gcagcagctg gaccgcggcg gggacgcccg agaacgcggc gaggtggagc
     1081 gcgctcgcgc cgaactcggt ccacgccgtc gccagcttcg gatcgcgctt gagctgccgc
     1141 gcgatctccg cggtatcgcc gaccccgcag gcctcgaaga aggtgagcgc cgggtgctgg
     1201 gcgagcagcg cctggagcgc cttgttctcg gccggcgggg tgaagccctc gccgtcgttg
     1261 tgcaacagcg ccttcaggat cggggagcga tcggcgtgcg cggggggcgc cgccgccagc
     1321 gagccggcga gcagcaggag gagggtgagc gtgatcgtct ggatcgacat gagatcacgc
     1381 tagcgatccg cgatcggcgg cgcatgtgcc gggcggcccg cggctgcgta tgacttccgg
     1441 cctatggcca gggccgcgcg gatccggcca agataacggg gtgaccgtgc gcgtccgtcc
     1501 gcacccgagc ttgctgctgg cggcgctcgc cgcgtggctg gcggtgtcgc tgccgacctg
     1561 gctcaccgcc caggcctcgc cgcggtttcc gctgtgggcg atcgcgtggg cggtgtgcgg
     1621 cgcggcgatg gtcgcggcgt cgctccggcc gcggctcgat gcgatcgcgg ccgtcgcgct
     1681 cgccgtgcag ggcgccgcgg tcgcgaccat ggtgggcctt ctgtgcaacg ggtt
//
LOCUS       DASVOR010000252         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353066,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000252 DASVOR010000000
VERSION     DASVOR010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353066"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF516_05425"
     CDS             complement(<1..342)
                     /locus_tag="VF516_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107147.1"
                     /translation="MAALALVLIADPLEPEQRRRLPLAARRVDALGDGARPRLVHVAK
                     QVRGEAAWRKDLSVEKPARFRAQPARRMMSEQEGGVAARGPSIEVAGNGGLRLTGMDR
                     EPLLEVGAGELE"
     gene            816..1889
                     /locus_tag="VF516_05430"
     CDS             816..1889
                     /locus_tag="VF516_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107148.1"
                     /translation="MTRSGAASTARWRCSIGSANSRDAVRLDDDLWVTTNSDGLLLAD
                     GIAAEEALPHLILNLRRERRPRLSVDRKKAIAWSRQWVGETLRSQWSKLVDWSPDFTT
                     LWWLDVAYPDVALRLTEALVQRGTAHRLPYGESPVRLDDVGCCAGDARLFEWLAEWPA
                     AYRRSGRVGIPESTDRWISSLPFPREGALGFAAQRLELWTRGGVQIPSAALQAVRIDM
                     HGQVPPLATVARCIPQAGDWFVLTQTQGGKYPAGLGPLLAAVLEEEDADLFATARRLG
                     LLPAACSDDIIAGLAIDEVDACILSRDGDGQDPWIDRKVPRSHIGHVAREQNLDEQDL
                     VRRVQRMAPLGVELLEKGKLQVL"
     gene            complement(2097..>2333)
                     /locus_tag="VF516_05435"
     CDS             complement(2097..>2333)
                     /locus_tag="VF516_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107149.1"
                     /translation="HAEIITSPRQARHALSYVLSNWRRHQEDLRAPMSGWTIDWFSSA
                     VMFSGWAEYGDGRFCGAALQPTTHSWCTNPEPGC"
BASE COUNT          360 a          810 c          790 g          373 t
ORIGIN      
        1 ctccagctca ccggcgccga cttcaaggag cggctcgcga tccatccccg tcaggcgcag
       61 cccgccgttg ccggcgacct cgatcgatgg gcctcgagct gccacgccgc cttcctgctc
      121 actcatcatc cgccgagctg gctgtgcccg gaagcgcgcg ggcttctcga cggagagatc
      181 cttccgccag gccgcttcac cgcgcacctg tttggccaca tgcacgaggc gcgggcgagc
      241 accgtctccg agggcatcga cgcgcctcgc cgcgagtggc aagcgacgtc gctgttcggg
      301 ctcgagtgga tcggcgatca gaacaagcgc gagcgccgcc acggctatac cgccggccgg
      361 atcacgctcg acggcgcggc cgcctcgatc cgccagtggc cgcgccgcgc ggagctcggg
      421 cagtcgcggg cgcgccacat cggccccgac acgtcgtacc gggctctgga tgatgcacca
      481 ctgcctggcc cgcgacccgg cgctgtggga gctctcgcga tgtctgttcg ccgatcccga
      541 gcggatcgac cgcgacgacc gcgacgaccg cctgcgcgac gaggagcgaa tcgctgaagg
      601 cgtctcggtg cgcgagaagg tgctcggcta cgcgctctgc ctcgccggct ggatggcgat
      661 ggacgcgcgc tgcctgcccg gggccatcat cgagcacatc ggcctgtccg acgcgatgac
      721 gccggccgac gtccatcagg cgcgtcgcgt cgcgtcgtgg cagccacggc tggctgggct
      781 cgcgctgacc gtgtcgtgcg cgcacccggc gatcgatgac gcgttccggc gcagcgtcga
      841 ccgcgcgatg gcggtgctcg atcggatccg cgaactcccg cgacgccgtg cgtctggacg
      901 acgacttgtg ggtcaccacg aactcggacg ggctcctcct cgccgacggc atcgcggcgg
      961 aagaggcact gccgcacctg atcctgaacc tgcggagaga gcggcgcccc cggctctcgg
     1021 tggatcggaa gaaagccatc gcctggagcc ggcagtgggt cggcgagacc ctccgcagcc
     1081 agtggtcgaa gctggtcgac tggtcaccgg acttcacgac cctctggtgg ctggacgttg
     1141 cctatcccga cgtggccctc cggctcaccg aggccctggt gcagcggggg acggctcaca
     1201 ggctgcctta cggggagtca ccggtccgac tcgacgacgt gggctgctgc gccggagacg
     1261 cccggctgtt cgagtggctc gcggagtggc ccgcggcgta tcggcgcagc ggacgagtcg
     1321 ggatccccga gtcgacggac agatggatca gttctctgcc tttccctcgc gagggggccc
     1381 ttgggttcgc agcgcaacgg ttggagctgt ggacgagggg tggcgtccag atcccctctg
     1441 ccgcgctgca agccgtccgc atcgacatgc atggtcaggt tccaccactg gcgaccgtgg
     1501 cccggtgcat cccgcaagcc ggagactggt tcgtcctcac acagacacag gggggcaagt
     1561 accccgcagg actcggaccg ctgctcgccg ccgtcctgga ggaagaggac gcagatttgt
     1621 tcgccaccgc tcgacggctc ggcctgctgc ccgccgcctg ctccgacgac atcatcgccg
     1681 gattggcgat cgatgaggtc gatgcgtgca tcctgtcgcg cgacggcgat gggcaggatc
     1741 cgtggatcga ccggaaggtg ccacggtctc acatcggcca cgtcgcgagg gagcaaaacc
     1801 tcgacgaaca ggacctggtg cggcgggtgc aacggatggc gccgctcggg gtcgagttgc
     1861 tcgaaaaggg taagctccag gtgctgtaga ccgcggcgac ggggcatccc tctgaccgag
     1921 catctctttc atgtctcccc cggggggcca atcccgccga catcgcacgc cgccgacatc
     1981 cggattctca tgcaggctcc cttctggcga tcctatcgtg ctggcgagtc atgatcaccg
     2041 ctttgctcct gggacctcat gacaggagat tgctccagcc ttcttccaac cttctctcag
     2101 cagccaggtt ctgggttggt acaccacgaa tgggtcgtag gttgcagggc cgcgccacag
     2161 aaacggccgt cgccatactc cgcccagccc gaaaacatca ccgcgctgga gaaccagtcg
     2221 atcgtccagc cggacatcgg cgcccggagg tcctcctggt ggcggcgcca attcgaaagg
     2281 acgtaggaca gagcgtgtcg cgcttgccgc ggtgaggtga tgatctccgc gtg
//
LOCUS       DASVOR010000253         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_90105,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000253 DASVOR010000000
VERSION     DASVOR010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90105"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="VF516_05440"
     CDS             complement(<1..245)
                     /locus_tag="VF516_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107150.1"
                     /translation="MATTDRFERNARAWQRPGLVLFAVAWGANHFVPLLLVYRARLAL
                     DAAQLALLFGMYALGLVPGLLLAGPVSDRRGRRAVALP"
     gene            complement(349..>1524)
                     /gene="hflX"
                     /locus_tag="VF516_05445"
     CDS             complement(349..>1524)
                     /gene="hflX"
                     /locus_tag="VF516_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEX8107151.1"
                     /translation="SFEAEYGRGRRAAATDRGLARALVVHVAVGRGVQSETRIAEVRE
                     LCRTAGVKVLDVMIQNRPEADPKFLVGRGKLDEILLRAMQLGAELVIFDPDLTPGQAR
                     AISAATELKVIDRTMLILDIFAQHATSRDGKLQVELAQLRYALPRLVEKNTMMSRLTA
                     GPGGKAGIGGRGPGETKLEINRRRARERINLLEKRLEELADDRRLRRQRRTDRDVPII
                     AICGYTNAGKSTLLNGLTEGDALAADQLFATLDPISRRLRFPHEREVVITDTVGFIRD
                     LPAELTRAFRATLEEMADADLLVHVVDASDPDREQQIRTVEGILADLGLTDKPRILVW
                     NKADRLAADEAEHLANGDGGFVVSALDRATFGPLLLALERTLWQHGKDTSLARVAHG"
BASE COUNT          208 a          530 c          578 g          208 t
ORIGIN      
        1 ggcagcgcca ccgcgcgccg gccgcggcga tccgacaccg gcccggcgag cagcagcccc
       61 gggaccaggc cgagcgcgta catcccgaac agcagcgcga gctgcgcggc atcgagcgcc
      121 agccgcgcgc ggtagaccag cagcagcggc acgaagtggt tggcgcccca ggcgaccgcg
      181 aacagcacga ggccaggccg ctgccacgcc cgcgcgttgc gctcgaaccg gtcggtggtg
      241 gccatcacgt ccggtggcag catctcattt cgatgccggg gcgcaagccg cggtcgcgtg
      301 ccacaccgca ggagatcgga catcgtggtg ctcgccgagt gacggccgct agccgtgcgc
      361 gacgcgggcg agcgaggtgt ccttgccgtg ctgccacagg gtgcgctcga gcgcgagcag
      421 cagcggaccg aacgtggcgc ggtcgagcgc ggacaccacg aagccgccgt cgccgttggc
      481 caggtgctcg gcctcgtcgg cggcgagccg gtcggccttg ttccacacca ggatgcgcgg
      541 cttgtcggtg aggccgaggt cggcgaggat gccctcgacg gtgcggatct gctgctcgcg
      601 atcggggtcc gaggcatcga cgacgtggac cagcaggtcg gcgtcggcca tctcctcgag
      661 cgtggcgcgg aacgcgcggg tcagctcggc cggcaggtcg cggatgaacc cgacggtgtc
      721 ggtgatcacg acctcgcgct cgtgggggaa ccgcagccgg cgcgagatcg ggtcgagcgt
      781 cgcgaacagc tgatcggcgg cgagcgcgtc gccctcggtc aggccgttga gcagggtcga
      841 cttgccggcg ttggtgtagc cgcagatcgc gatgatcggc acgtcgcgat cggtgcggcg
      901 ctggcggcgc agccggcgat catcggcgag ctcctcgagc cgcttctcga gcaggttgat
      961 ccgctcgcgc gcgcggcggc ggttgatctc gagcttggtc tcgcccgggc cgcgcccgcc
     1021 gatcccggcc ttgccgccgg ggccggcggt cagccgcgac atcatggtgt tcttctcgac
     1081 cagccggggc agcgcgtagc gcagctgggc gagctcgacc tggagcttgc cgtcgcggct
     1141 ggtcgcgtgc tgggcgaaga tgtcgaggat cagcatcgtg cggtcgatca ccttgagctc
     1201 ggtggcggcg ctgatcgcgc gggcctgacc gggcgtcagg tccgggtcga agatcacgag
     1261 ctcggcgccg agctgcatcg cgcgcagcag gatctcgtcg agcttgccgc ggccgaccag
     1321 gaacttcgga tcggcctcgg gccggttctg gatcatcacg tcgaggacct tcacgccggc
     1381 ggtgcggcac agctcgcgca cctcggcgat gcgggtctcc gactgcacgc cacggccgac
     1441 cgcgacgtgc accaccagcg cccgcgccag gccgcgatcg gtcgccgcgg cgcgccggcc
     1501 acggccgtac tcggcctcga acga
//
LOCUS       DASVOR010000254         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_60777,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000254 DASVOR010000000
VERSION     DASVOR010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60777"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF516_05450"
     CDS             complement(<1..617)
                     /locus_tag="VF516_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107152.1"
                     /translation="MRFLRATTLAALLGAPLGLAACGNSERAGAEEAQRQAEQEQKAR
                     QAASPPAQRIAPPVPGQAKIPCSQLIDAAAFQTALGEKLPLTVKDVTRSEPDSVASCS
                     LVRGGKPLTAAEQKALLQKSGRLGVLPGDELCNVTAFCSTIEDPDRFRATCKQHKDRD
                     DESLGSYACVHIQPVGADDVKLFRLLDDDTRCILQIRGGPSNVNN"
     gene            852..925
                     /locus_tag="VF516_05455"
     tRNA            852..925
                     /locus_tag="VF516_05455"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:886..888,aa:Arg,seq:tct)
     gene            956..>1517
                     /locus_tag="VF516_05460"
     CDS             956..>1517
                     /locus_tag="VF516_05460"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8107153.1"
                     /translation="MTDHVVDKHPITESLGGRPRASVVKPHTGSKVKRFFVHLYHELA
                     NDNIVDIGAMMAYYAILALFPMLVFVVTLTLLVLPGDTLVQGLAMVTRAMPEAPREVI
                     TTQVESLMKQAHAGFAIGTFALALWGASRGASSLMGALNAMFNKRETRPWWKRQVIAI
                     TVTACVALLAVVALGLLVVGPIAGHWL"
BASE COUNT          211 a          495 c          567 g          244 t
ORIGIN      
        1 tcgttgttga cgttgctcgg gccgccgcgg atctggagga tgcagcgggt gtcgtcgtcg
       61 agcagccgga acagcttgac gtcgtcggcg ccgaccggct ggatgtggac gcaggcgtag
      121 ctgccgagcg actcgtcgtc gcgatccttg tgctgcttgc aggtggcgcg gaaccgatcc
      181 gggtcctcga tcgtggagca gaacgcggtc acgttgcaca gctcgtcgcc cggcaggacg
      241 ccgagccggc cggacttctg gagcagcgcc ttctgctcgg cggcggtcag cggcttgccg
      301 ccgcggacca gcgagcacga cgccacggaa tcgggttcgc tccgggtgac gtccttgacg
      361 gtcagcggca gcttctcgcc gagcgcggtc tggaacgccg ccgcgtcgat cagctggctg
      421 cacgggatct tggcctggcc gggcaccggg ggggcgatcc gctgggccgg tgggctggcg
      481 gcctgccggg ccttctgctc ctgctcggcc tggcgctgag cctcctccgc gccggcgcgc
      541 tcgctgttgc cgcaggcggc caggcccagg ggcgctccga ggagggcagc gagggtcgtc
      601 gcgcgcaaga aacgcatgcg aagcgaagct aacacggcgg cgggacgccg caccggggcc
      661 gtggcgctcg gcacatcggc acggtcagcc cggacggtga accgcatccg agcgggtggg
      721 cacccaggct gcgggctggt ggaccgtccg gggggccggg cctcggcggc gggcgttgca
      781 gaggggttgc cggggttgag agatccgcca acctggggca aagtgcgaga ccggctctcg
      841 gatccgcatc cgcgcccata gttcagccgg atagaacaac cgccttctaa gcggtaggtc
      901 gcaggttcga ttcctgctgg gcgcgctggc acaatcgctg ctccacccgg atggtgtgac
      961 cgaccacgtc gtggacaagc atccgatcac cgagagcctc ggcgggcgtc ctcgcgcgtc
     1021 cgtggtgaag ccgcacaccg gtagcaaggt gaagcggttc ttcgtgcacc tctaccacga
     1081 gctggccaac gacaacatcg tcgacatcgg ggcgatgatg gcctactacg cgatcctggc
     1141 gctgttcccg atgctggtgt tcgtcgtgac gctcacgctg ctggtactgc ccggcgatac
     1201 gttggtgcag ggcctggcga tggtgacccg cgcgatgccg gaggcgccgc gcgaggtgat
     1261 cacgacccag gtcgagtcgc tgatgaagca agcgcacgcc ggattcgcga tcggcacgtt
     1321 cgcgctcgcg ctgtggggcg cgtcgcgcgg ggcgagctcg ctgatgggcg cgctcaacgc
     1381 gatgttcaac aagcgcgaga cccggccgtg gtggaagcgc caggtcatcg cgatcaccgt
     1441 gacggcctgc gtcgcgctcc tcgccgtcgt ggcgctcggc ttgctcgtgg tcggtccgat
     1501 cgccggacac tggctcg
//
LOCUS       DASVOR010000255         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382218,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000255 DASVOR010000000
VERSION     DASVOR010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382218"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..824)
                     /locus_tag="VF516_05465"
     CDS             complement(81..824)
                     /locus_tag="VF516_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107154.1"
                     /translation="MNELLRRVLHLPAQSSSVARDIDALHYTVILATMAGVTLAAVIA
                     GVFLVRYRRRTPVAPLTPRITVPLWFEVVVYGGLLILFCAWWVVGFAQYRDIETAPAD
                     AMPIYVTAKQWMWKFAYPDGPTSQDALIVPVGRPVKLILTSRDVIHSFYVPEFRLKQD
                     VVPGRAETLWFEATAPGSYDVLCAQYCGLRHSLMRCFRYDPANRRYGLFVARFMQLGD
                     AVVLAIVLAMAWVFARHKLRRARSREVLP"
     gene            complement(821..>1526)
                     /locus_tag="VF516_05470"
     CDS             complement(821..>1526)
                     /locus_tag="VF516_05470"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX8107155.1"
                     /translation="GARLPPELGFVDARGRRVPLGSLFDGAHPVLLVLAYYECPQLCS
                     LVLDGATRAMRQLEAQGFALGRQYRAVTISFDATERIDQAARKQASVLAGLGRRDPAD
                     WPFLVGDDASVRALTARLGFEFLRDPQTGAIAHPAVAFVVTPDGAIARYLYGIDYPAR
                     DLRLALLDASDGKTGSFGDRVLMRCFRYDPATRRYGLFVARFMQLGGAVILAIVLAMV
                     WVFTRHELRRAREVVP"
BASE COUNT          253 a          521 c          578 g          174 t
ORIGIN      
        1 ggtgtagtgc agcgcatcga tgtcgcgcgc gaccgacgag ctctgcgccg gcaggtggag
       61 gatgcggcgc aacagctcgt tcatggcagg acctcccgcg accgcgcccg gcgcagcttg
      121 tggcgcgcaa acacccaggc catcgcgagg acgatcgcga ggacgacggc gtcgccgagc
      181 tgcatgaacc gcgcgacgaa cagcccgtag cgccggttcg ccggatcgta gcggaagcag
      241 cgcatcagcg agtgccgcag gccgcagtac tgcgcgcaca gcacgtcgta gctgccgggc
      301 gcggtggcct cgaaccacag cgtctcggcc cggccgggga ccacgtcctg cttgaggcgg
      361 aactcgggga cgtagaagct gtggatcacg tcgcgcgagg tcaggatcag cttgaccggc
      421 cgcccgaccg ggacgatcag cgcgtcctgc gaggtcgggc cgtcgggata ggcgaacttc
      481 cacatccact gcttggcggt gacgtagatc ggcatcgcgt cggccggcgc ggtctcgatg
      541 tcgcggtact gggcgaagcc gaccacccac cacgcgcaga acaggatcag cagcccgccg
      601 tacaccacga cctcgaacca cagcggcacg gtgatccgcg gcgtcagcgg ggccaccggc
      661 gtgcggcggc gatagcggac gaggaacacg ccggcgatca cggcggcgag cgtgacgccg
      721 gccatggtcg cgaggatcac ggtgtagtgc agcgcatcga tgtcgcgcgc gaccgacgag
      781 ctctgcgccg gcaggtggag gacgcggcgc agcagctcgt tcatggcacc acctcccgcg
      841 ccctgcgcag ctcgtggcgc gtgaacaccc agaccatcgc gaggacgatc gcgaggatga
      901 cggcgccgcc gagctgcatg aaccgcgcga cgaacagccc gtagcgccgg gtcgccggat
      961 cgtagcggaa gcagcgcatc agcacccggt cgccgaacga gccggtcttg ccgtcggagg
     1021 cgtcgagcag cgcgagccgg agatcgcgcg cggggtagtc gatgccgtag aggtagcgcg
     1081 cgatcgcgcc gtcgggggtc accacgaacg cgaccgcggg atgggcgatc gcgccggtct
     1141 ggggatcgcg caggaactcg aagccgagcc gggcggtcag cgcgcgcacg ctggcgtcgt
     1201 cgccgaccag gaacggccag tcggccggat cgcggcggcc gagcccggcg agcacgctgg
     1261 cctgcttgcg cgccgcctga tcgatccgct cggtggcgtc gaagctgatc gtgaccgcgc
     1321 ggtactggcg gccgagcgcg aagccctggg cctcgagctg gcgcatcgcg cgggtcgcgc
     1381 cgtcgagcac caggctgcac agctgcgggc actcgtagta ggcgagcacg agcagcaccg
     1441 ggtgcgcgcc gtcgaacagc gagccgagcg ggacgcgccg gccgcgcgcg tcgacgaagc
     1501 cgagctcggg cggcagccgg gcgccg
//
LOCUS       DASVOR010000256         2592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_294662,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000256 DASVOR010000000
VERSION     DASVOR010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294662"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF516_05475"
     CDS             <1..190
                     /locus_tag="VF516_05475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107156.1"
                     /translation="GLPAVQQVLDQRAQAVRANAAYLGPSAVEALDRAQAQTIDGFAR
                     APAAAKKAASVTAHDLAR"
     gene            complement(214..1062)
                     /locus_tag="VF516_05480"
     CDS             complement(214..1062)
                     /locus_tag="VF516_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107157.1"
                     /translation="MKLFACICNQPQRLPIALAPVRAALIAQPPVSRWGLGYVQGGDV
                     LLVRTPKASAVPVDLAGPLTAEIKTDCVIAQAVRDDGPGLGGTDNTPPFRFRRWMYAQ
                     SGLATQLFSGVVGAAPTDGAAAGIEEVMPRLLEHIPEYLRRNLKGRTPAELIFHVFLA
                     MLHDEGNIDDANLPPAASRRALAATLKLVAAELARAGKTEAALGNVALTNGRSMVVAH
                     LDDPLRLRRLWVTGERGERDESFRGFLLVSGGDGDAKDGFEDVPAHRSVLISRDLQVS
                     FADLAP"
     gene            complement(1416..2273)
                     /locus_tag="VF516_05485"
     CDS             complement(1416..2273)
                     /locus_tag="VF516_05485"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8107158.1"
                     /translation="MTALPGPIAPSSTRTVGGLYTESFVPAGAPRGVVLINHGYAEHC
                     GRYHEVAHVLVNAGWAAFSYDVRGHGKSPGERGYIERFAIYLDDLAAMLDAARALAAA
                     DAPLVLLGHSHGGLIALRALADDRPPRVAGAILSSPFLALRLAVPGFQLLLARVASRL
                     APKLAQPNALRVEDLTQDKAKQAERIADKLCFDVATARWFTEARAAQDHVFAHADRIG
                     VPTTWLVGGDDPIADPARSRAVAAKIAGASYHDLVGMRHEVFNEIERGKVFSELSQAL
                     DAAATAARR"
     gene            2272..>2592
                     /locus_tag="VF516_05490"
     CDS             2272..>2592
                     /locus_tag="VF516_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107159.1"
                     /translation="MKNFYIATGTATRPPGWLDPHPGQAVRKLPGGRRSAYRDAMVRN
                     TLGLLAIFAVAGCAMHDERDPAKSATRLDLAKDFLRKHQLEAADSECSRALAFNPAND
                     EAYNV"
BASE COUNT          412 a          912 c          920 g          348 t
ORIGIN      
        1 cggactgccg gcggtgcagc aggtgctcga ccagcgcgcg caggcggtgc gcgccaacgc
       61 ggcgtacctc ggcccgagcg cggtcgaggc gctcgaccgg gcgcaggccc agacgatcga
      121 cgggttcgcc cgcgcgccgg cggcggccaa gaaggccgcg agcgtgacgg cgcacgatct
      181 ggcgcgctga gccgcgctac gcccggcaga cgcctacggg gcgagatcgg cgaacgagac
      241 ctggagatcg cggctgatca gcaccgagcg gtgtgcggga acgtcctcga agccgtcctt
      301 ggcatcgcca tcgccgcccg acaccagcag gaagccgcgg aacgactcgt cgcgctcgcc
      361 gcgctcgccg gtcacccaca gccggcgcag ccgcagcgga tcgtcgaggt gggcgaccac
      421 catcgaccgc ccgttggtca gcgcgacgtt gcccagcgcg gcctcggtct tgccggcgcg
      481 cgcgagctcg gcggcgacca gcttcagcgt cgccgcgagc gcacggcgcg acgccgccgg
      541 tggcaggttg gcgtcgtcga tgttgccctc gtcgtgcagc atcgcgagga acacgtggaa
      601 gatcagctcc gccggggtcc ggcccttgag gttgcgccgc aggtactccg gaatgtgctc
      661 caggaggcgc ggcatcacct cttcgatccc ggcggccgct ccatcggtgg gggcggcccc
      721 caccacaccg ctgaacagct gggtggcgag tccgctctgc gcgtacatcc agcgccggaa
      781 ccggaacggc ggcgtgttgt cggtcccgcc caggcccggg ccgtcatcgc gcacggcctg
      841 ggcgatcacg cagtcggtct tgatctcggc ggtcagcggc ccggcgagat cgaccgggac
      901 cgcgctcgcc ttcggcgtgc gcaccagcag gacatcaccg ccctggacat accccaggcc
      961 ccagcggctc acgggcggct gtgcgatcaa cgccgcacgc accggtgcga gcgcgatcgg
     1021 cagtcgctga ggttggttgc aaatacaggc gaagagtttc attgcgggag actccgcagc
     1081 cgggacgaac aggccgccgg ctgctgatga tcaacgatcg cgaagcccag aggggcaaag
     1141 aacgacgtca cgacttccac cccaaggaac gtcacctctc gaggatacca ggagtatagg
     1201 ggtccggtcg gacctcgcaa tctccgcggc gtaacgtcgc gccgcccctg cgtccgccca
     1261 tgccaagcgt gtgcgacctc gcggcacgct aaccgatcga tgcgacaaat tttcggggca
     1321 acgctcgcac gccgggtagg ccgggcggcc accgtgagcc gggtgcgccc gtcgcgcccc
     1381 gcgagcgagc cagtgcggag ccgtccggag atcgatcagc gccgcgcggc cgtcgctgca
     1441 gcatcgagcg cctgcgacag ctcagagaat accttgccac gctcgatctc gttgaacact
     1501 tcgtgccgca ttccgacgag gtcgtggtag ctcgcaccgg cgatcttcgc ggcgaccgca
     1561 cgcgatcgcg ccggatccgc gatcggatcg tcaccgccga ccaaccatgt ggtcggtacg
     1621 ccgatccggt ccgcgtgtgc gaacacgtgg tcctgcgcgg cgcgcgcctc ggtgaaccag
     1681 cgcgcagtcg cgacatcgaa gcacagcttg tcggcgatcc gctcggcctg cttggccttg
     1741 tcctgcgtga gatcctcgac gcgcagcgcg ttgggctgcg ccagcttggg agcgagccgg
     1801 ctcgcgacgc gggcgagcag cagctggaag cccggcaccg cgagccgcag ggcgaggaac
     1861 ggcgacgaca ggatcgcgcc ggcgacccgg ggcggacggt cgtcggcgag cgcgcgcagc
     1921 gcgatcaggc caccgtgcga gtggccgagc aggaccagcg gcgcatcggc ggcggccagc
     1981 gcgcgggcgg cgtccagcat cgcggcgagg tcgtcgaggt agatcgcgaa ccgctcgatg
     2041 tagccgcgct cgccgggcga cttgccgtgg ccgcgcacgt cgtacgagaa cgcggcccag
     2101 ccggcgttca cgagcacgtg ggcgacctcg tggtagcggc cgcagtgctc ggcgtagccg
     2161 tggttgatca gcaccacgcc gcgcggcgct ccggccggta cgaagctctc cgtgtacagc
     2221 ccaccgaccg ttcgggtcga gctgggcgcg atcggtcctg gcaaggcggt catgaagaac
     2281 ttctatatcg caaccgggac ggcgacccgg ccccccggct ggctcgaccc gcatccgggc
     2341 caggctgtcc ggaagttgcc cggcggccga agatctgcgt accgtgacgc catggtgcgt
     2401 aacacgcttg gattgctggc gatttttgcg gtggccggat gcgcgatgca tgacgagcgc
     2461 gatcccgcga agagcgccac ccgcctcgac ctggccaagg acttcctgcg caagcaccag
     2521 ctggaggccg ccgatagcga atgcagccgc gcattggcgt tcaatccggc caacgacgag
     2581 gcatacaatg tc
//
LOCUS       DASVOR010000257         3995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148364,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000257 DASVOR010000000
VERSION     DASVOR010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148364"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..789)
                     /locus_tag="VF516_05495"
     CDS             complement(163..789)
                     /locus_tag="VF516_05495"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX8107160.1"
                     /translation="MRHLIATLCLSLATFAVGQAWSAPTKPAAPAKPAPAPAPDQPPP
                     KLTVAQILDRQLTNLEKEFVPAAEAMPDDKFGFAPTQGEFKDVRTFALQIKHVAHTNF
                     LMFAMLLGEKLPANVDPKEDNGPDKMTSKADILKYLKESFAMGHRAMATLSDANLTER
                     VAGPDGKPGPTRLGAASLTVWHSFDHYGQMVEYLRMNGIVPPASRTPQ"
     gene            1233..1682
                     /locus_tag="VF516_05500"
     CDS             1233..1682
                     /locus_tag="VF516_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107161.1"
                     /translation="MVATPSPLKVDPELRHQVFGYWFGPDEDIPDRPIGPPTGAVLPH
                     PGSSFREDAELLCLDLIPIDNYWIVLSELGRYTYGHTSGLLYDEEWVFVPVRDAVDGK
                     WPVRFLATYAESVEPSELAQYGEEVPTRARHDAPAVQLRLRRIANGG"
     gene            1621..2292
                     /locus_tag="VF516_05505"
     CDS             1621..2292
                     /locus_tag="VF516_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107162.1"
                     /translation="MRGTTRRRSSCACAASRMADSWSEKLAQSWLASPEARLAMDAKL
                     AQLMAVHWGRRGAGVTAERLLPVRCETTAASLITIGKQPDAAMSCTLLLVMLGELLLC
                     AEHGSILRDDVMVERFAGRSDERWLAAEALRRLRNAACHPAAVAASAKGEVAIVSFAE
                     FLIMNFKEYTWAQGLRTQPGLLAHRAVSLFALRMVDSIGRSRAGHWGVPFPGLKPPGP
                     LRSRS"
     gene            complement(2333..3529)
                     /locus_tag="VF516_05510"
     CDS             complement(2333..3529)
                     /locus_tag="VF516_05510"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEX8107163.1"
                     /translation="MTVSPLADALQGVRVLDMSRLLPGPFLTMILADMGADVVKIEDP
                     HAGDYMRDVPPPIGDIPMHQLVPGKGGISGRFLAVNRGKRSTVLDLKKPSGRAAMLRL
                     VAGADVVVESFRPGVMDKLGLGYARLAAANPGIVLCSISGFGQTGPYVHRAGHDIGYL
                     AVAGVLAMGGEHGGAPMMPGVQIADLAGGALWGATAILGALVGRHRTGKGAHLDISMT
                     EGALALLAAELGNLDCGLRATRGMARLNGGFACYGIYRTKDSRYLAVGALEPKFWIAL
                     NQGVGRRPDVAELLAADQAAIRNELAAIFAAKTAAEWTAILGQHDCCVELVTELDELP
                     DHPLHRAREVFFTIDGGAGVGPIQQTRTPLGIPKAPRPPPRHGEHTREVLAEYGFSDA
                     DIAQVT"
     gene            complement(3540..3893)
                     /locus_tag="VF516_05515"
     CDS             complement(3540..3893)
                     /locus_tag="VF516_05515"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8107164.1"
                     /translation="MATLPKDAQELFDNLVPEGLRQFPDKARELNAIYCFKITGEGGG
                     EWTVDLTANPPTCTKGDSGKAQCTVEVSNDDFKTMLGDPNAGMQLYFQGKLRVSGDPM
                     LAMKLQQLFEIARPK"
BASE COUNT          558 a         1349 c         1449 g          639 t
ORIGIN      
        1 caccgcgagc agcacaccgc gcgagatcgc cgagccggcc atgcctgact gtcgcacgcc
       61 gagcggctca ccgcagcgat gcggcggatc ctgcgagggc tccggggaac cgaacgagac
      121 ccgctcgcca tcgcgatcgc gggcggcgcc ggcccaggtc cgctactgcg gggtgcggct
      181 cgccggcggg acgatgccgt tcatccgcag gtactcgacc atctggccgt agtggtcgaa
      241 gctgtgccac acggtgaggc tggcggcgcc caggcgggtc ggccccggct tgccgtccgg
      301 cccggcgacg cgctcggtga ggttcgcgtc ggacagcgtg gccatcgcgc ggtggcccat
      361 cgcgaacgat tccttgaggt acttgaggat gtcggccttc gacgtcatct tgtccggccc
      421 gttgtcctcc ttggggtcga cgttggccgg cagcttctcg ccgagcagca tcgcgaacat
      481 caggaagttg gtgtgcgcga cgtgcttgat ctggagcgcg aacgtgcgca cgtccttgaa
      541 ctcgccctgg gtcggcgcga agccgaactt gtcgtcgggc atcgcctcgg ccgccggcac
      601 gaactccttc tcgaggttgg tcagctggcg atccaggatc tgcgcgaccg tcagtttcgg
      661 cggcggctga tcgggcgccg gcgccggcgc cggcttggcg ggtgcggcgg gcttggtcgg
      721 ggcggaccag gcctgtccga cggcgaaggt ggcgaggctg aggcacaagg tggcgatcag
      781 gtggcgcatc ggattcctcc agggagcaga caccgcgttc gcgatctgcc tagcacggct
      841 ccgcgccggc actcgatcac gcgtgctggc cgcatcgcga accggccgct gcgcgcgaca
      901 ccgaaggccg cgccgcgctg tcgcagcgcc ccggcgagcg gtcgcaccag acgcagccgc
      961 gcgtggccgt ggcagcagcc ttgagtatca tctcctcctc gatgccttcg gtcgctccac
     1021 cagcggacga tccgcagccg ccgtgctgcg gcaggcaacc tccagcgcgg cctgcccgcg
     1081 aacggcatct gctggttctt gagctcgtgg atcgcgatcc aagtggaagg tgcagccggg
     1141 aactcatcac tggagctcac agctgccact caccctcgcg actccgcggt gagtgagtgt
     1201 tcactgagga gccgcgaccc tatagttatc atgtggtcgc gactccatcc ccgttgaagg
     1261 tcgatcccga gctgcggcat caggtcttcg ggtactggtt cggccccgac gaggacattc
     1321 cggatcggcc gatcggaccg ccgaccgggg cggtcctgcc gcatcccgga tcgagctttc
     1381 gcgaggatgc ggagctcctg tgcctcgacc tcatccccat cgacaactac tggatcgtgc
     1441 tgtccgagct gggtcgttac acgtacggcc acacgtccgg cctgctgtac gacgaggagt
     1501 gggtcttcgt tccggtgcgc gacgcggtgg acggcaagtg gcccgtacgc ttcctcgcca
     1561 cgtacgcgga gtccgtggaa ccctcggagc tcgctcaata cggcgaagag gttccgaccc
     1621 gtgcgcggca cgacgcgccg gcggtccagc tgcgcctgcg ccgcatcgcg aatggcggat
     1681 agctggtccg agaagctcgc gcagagctgg ctggcttccc cggaggcacg cctcgcgatg
     1741 gacgcgaagc tggcacagct gatggcggtg cactgggggc ggcgcggcgc cggtgtgacc
     1801 gccgagcggc tgctcccggt gcgctgcgag accacggcgg cttcgctgat cacgatcggc
     1861 aagcagcccg atgcggcgat gtcctgcacg ctcctgctgg tcatgctggg cgagctattg
     1921 ctgtgtgccg agcatggcag catcctgcgc gatgacgtga tggtagagcg gttcgcgggc
     1981 aggagcgacg agcgctggct cgccgccgag gcgctgcgcc ggcttcgcaa tgcagcgtgc
     2041 catcccgcag cggtggcggc ctctgcgaag ggcgaggtcg ccattgtctc gttcgcagag
     2101 ttcttgatca tgaacttcaa ggaatacact tgggcgcaag ggctgcggac gcaacccggt
     2161 cttctcgccc accgcgcggt ctccctgttc gcgcttcgca tggtcgactc gatcggccgg
     2221 tcgcgagccg gtcattgggg cgtgccattc ccagggctca agccgcccgg acccctgcgc
     2281 tcgcgcagct gacgggtacg gccctgcaga ccgccacggc atgcagcggc ggtcaggtga
     2341 cctgcgcgat gtcggcgtcg gagaacccgt actcggcgag gacctcgcgg gtgtgctcgc
     2401 cgtggcgcgg cggcggccgc ggcgccttcg ggatgccgag cggcgtgcgg gtctgctgga
     2461 tcggcccgac gccggcgccg ccgtcgatgg tgaagaacac ctcgcgggcg cggtgcagcg
     2521 ggtggtcggg cagctcgtcg agctcggtga ccagctcgac gcagcagtcg tgctggccga
     2581 ggatcgcggt ccactcggcg gcggtcttcg cggcgaagat cgcggcgagc tcgttgcgga
     2641 tcgcggcctg gtcggcggcg agcagctcgg cgacgtccgg ccgccggccg acgccctggt
     2701 tgagcgcgat ccagaacttg ggctccagcg cgccgaccgc gaggtagcgg ctgtccttcg
     2761 tgcggtagat cccgtagcag gcaaagccgc cgttgagccg cgccatgccg cgggtggcgc
     2821 gcaggccgca gtcgaggttg ccgagctcgg cggcgagcag ggcgagcgcg ccctcggtca
     2881 tcgagatgtc gaggtgggcg cccttgccgg tgcggtggcg gccgaccagc gcgccgagga
     2941 tcgccgtggc accccacagc gcgccgccgg cgaggtcggc gatctggacg ccgggcatca
     3001 tcggcgcgcc gccgtgctcg ccgcccatcg cgagcacgcc ggcgaccgcg agatagccga
     3061 tgtcgtggcc cgcgcggtgg acgtacgggc cggtctggcc gaacccgctg atcgagcaca
     3121 ggacgatccc ggggttggcg gcggcgagcc gggcgtagcc gaggccgagc ttgtccatca
     3181 cgccggggcg gaagctctcg acgacgacgt cggccccggc gaccaggcgc agcatcgccg
     3241 cgcgcccgga tggcttcttg aggtcgagca cggtcgagcg cttgccgcgg ttgaccgcga
     3301 ggaaccggcc cgagatgccg cccttgcccg gcaccagctg gtgcatcgga atgtcgccga
     3361 tcggcggcgg gacatcccgc atgtagtcgc cggcgtgcgg atcctcgatc ttgaccacgt
     3421 ccgcgcccat gtcggcgagg atcatggtca ggaacggccc cggcaggagc cgcgacatgt
     3481 cgagcacgcg cacgccttgc agcgcgtcag ccagcggaga gaccgtcacg cggcgcggct
     3541 cacttgggac gggcgatctc gaacagctgc tggagcttca tcgccagcat cggatcgccg
     3601 gacacccgga gcttgccctg gaagtacagc tgcatgccgg cgttggggtc gccgagcatg
     3661 gtcttgaagt cgtcgttgga gacctcgacg gtgcactgcg ccttgccgct gtcgcccttg
     3721 gtgcaggtcg gcgggttggc ggtgaggtcg accgtccact cgccgccgcc ttcgcccgtg
     3781 atcttgaagc agtagatggc gttcagctcg cgcgccttgt ccgggaactg cctgaggcct
     3841 tccgggacca ggttatcgaa cagctcctgc gcatccttgg gcaacgtagc catgtgtgtc
     3901 ctccgccggc gtgttaccgc gggcgaagtt ttcagtcaac gctgactgcc gaaaacgcgg
     3961 gcggccccgc agggggccgt gcgcgtcggt gacac
//
LOCUS       DASVOR010000258         1625 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_557672,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000258 DASVOR010000000
VERSION     DASVOR010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1625
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557672"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VF516_05520"
     CDS             <1..405
                     /locus_tag="VF516_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107165.1"
                     /translation="AAPAPPPRDIARLTRRAATVLLAEARAAVAAIGDATTDAGAIDR
                     IADIAQDPPPGTTLVIAGDDDHTRGRYDGTARGLLHDLEPLGAIRSWGRFDRAGDGSW
                     QHRITVPVHRARPARELITRWLALTRIDAALE"
     gene            1046..1225
                     /locus_tag="VF516_05525"
     CDS             1046..1225
                     /locus_tag="VF516_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107166.1"
                     /translation="MLVTADPVVAELEAALALRRSSADPGALRRALRRIEELLDEWRA
                     ASRNRMASLPIRRSR"
     gene            1415..>1625
                     /locus_tag="VF516_05530"
     CDS             1415..>1625
                     /locus_tag="VF516_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107167.1"
                     /translation="MGRAITGCRRAPVVIRRRWARPAAARQAARALHRIGGHKEASMT
                     NARSWLRQLLIPILMVATLVGTVRIA"
BASE COUNT          249 a          560 c          573 g          243 t
ORIGIN      
        1 gccgcgcccg cgccgccacc ccgcgatatc gcgcgactga cccggcgcgc ggcgaccgtg
       61 ctgctcgccg aggcgcgagc cgcagtggcc gcgatcggcg acgccacgac cgacgccggt
      121 gcgatcgacc gcatcgccga catcgcccag gatccgccgc cgggcacgac gctggtgatc
      181 gccggcgacg acgaccacac gcgcgggcgc tacgacggca ctgcgcgcgg cctgctacac
      241 gatctcgaac cgctcggcgc gattcgctcg tggggccggt tcgaccgggc cggcgacggc
      301 agctggcagc accggatcac cgtacccgtc caccgcgctc gacccgcgcg cgagctgatc
      361 acccgctggc tggcgctgac ccggatcgat gcagcgctcg aatgatggag ccgcgcggac
      421 gccggtgatc gggtgctccg gcgcgccatg gtcgtcgagc tcgcgcatat cctacatcgg
      481 tcattcttgc gttgttgcgg cagcttccgt accatctatt cgtctcgccg acatatccag
      541 agaccacgag aggagcccca tgcgaacacg gagtcaactc gtcatcggtt cgtgcatcgc
      601 tatcgcagcg attgccctcg ttgcgggcag ccagggagac cccggatacg gcacgctccg
      661 gcggagcgat gcactcgtcc agcgtactcg cgtggccgac agagacctcg tcggcacgct
      721 cggagacggc agcaccgtcg gtatttcgtt cgctggtacc cacgccaacc cggtgcagaa
      781 tctgttgcag ctcagggatg aacaagtcga tctccagggg gggtgtgggg cgcagtgtgg
      841 gcgtatcgag ttttgctgtg gcggctgcca ggccggggag tgcctggagt gctgtccgct
      901 ctcgtcccca ccggtccctg accgcaccga atagagctgc cggtatcacc gacgagtgcc
      961 tgggtcagga gagcggcgcc gaaggggcgg acgcggcgca cggtcccgct gacggatccg
     1021 ctgctcgcga catggagcgc catcggtgct cgtgacagcg gatcccgtgg tcgccgagct
     1081 cgaggccgcg ctcgcgctgc gccggtccag cgccgatccg ggggccctgc gccgcgcgct
     1141 gcgccggatc gaggagctgc tggacgagtg gcgggcggcg agcaggaatc gcatggcgtc
     1201 gctacccatc aggcgctcga ggtagcgcag ccggtgaccg agatgggccc gcgacgagca
     1261 acgacaacgc ctggataccc atcgcatttc ccgctggcgg ggctccggca caggacgcgg
     1321 tcgagctcag catcgtgatc cgcgaccaca aggtcgtcct cgaggccaag ccgcgctgac
     1381 ggcgcgcggc tgctgcgtgc tccagcctgc agtgatgggc cgcgccatca ccggatgcag
     1441 gcgcgcgcct gtggtgattc gtcgacggtg ggcgcggccc gcagctgcgc gccaggcggc
     1501 acgcgcgctg catcgcatcg gcggccacaa ggaggcatcg atgaccaatg ctcgatcttg
     1561 gttacggcag ctgttgatac cgatactgat ggtggcgacg ctggtgggga cggtccgaat
     1621 cgccg
//
LOCUS       DASVOR010000259         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_440637,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000259 DASVOR010000000
VERSION     DASVOR010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440637"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1247)
                     /locus_tag="VF516_05535"
     CDS             complement(135..1247)
                     /locus_tag="VF516_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107168.1"
                     /translation="MRRAALPAIGILGVARIAAADPWTVRMESGAEADSNVTRTEDAP
                     IAAAVGRLGARITSRGPVLGGAYIVDVSGFARMVASSNPQIRDESVMLYVGELRWLHP
                     IGEGSLAAGVHLTAADSFGVLLPIGARTYRNLGGDALLVLGHDEDHHLTLGVGGRDFW
                     YKPHGPDLPPHIFDWRGPVANARLDAMLWHTPDRTRSLELAVALGFEARSYDSTATFN
                     CAPVDSGNPCPMDTTLQRRDRYQRAGLELTWTGDVVATGTYQVTVVDSNSYGQSLIRQ
                     RITAAATMEVVSALFISATATLQIDQYPEGVPNLVHQELTSLEDENRSSLQVLVTRAL
                     STAWSVEARGALWRDFGTDGASFGRELVYAGVLYQR"
     gene            1344..1673
                     /locus_tag="VF516_05540"
     CDS             1344..1673
                     /locus_tag="VF516_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107169.1"
                     /translation="MIASIFASIFVLGVGLAAGAGCRVDAQQCDQACRNAMSLAYWKA
                     ADQEIAAAAADQRDALRKQKASEFTQKVEAGIDGCVLRCQSARNQDAVDCLIAARTAD
                     QAQACTR"
     gene            1704..2048
                     /locus_tag="VF516_05545"
     CDS             1704..2048
                     /locus_tag="VF516_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107170.1"
                     /translation="MSSQPPGLLFHRRVDQLAARVRWLDRYRRFMAISAAALIAPVLI
                     AQVAGVLGADWPQLHATVLSVMLGVIVWWVIEVGLVYVTALWETEHDRLLRDRGLPRA
                     VLRRPRIRRSRS"
     gene            complement(2131..>3054)
                     /gene="groEL"
                     /locus_tag="VF516_05550"
     CDS             complement(2131..>3054)
                     /gene="groEL"
                     /locus_tag="VF516_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX8107171.1"
                     /translation="GKPLLIVAEDVDGEALATLVVNKLRGTLHVCAVKAPGFGDRRKE
                     MLKDLAVLTGGQAVTEDLGLKLENLTLQDLGQAKRITVDKDNTTVVDGAGKKSDIEAR
                     VKTIRKQIEETTSDYDREKLQERLAKLVGGVAIIRVGAATEVEMKEKKARVEDALHAT
                     RAAVEEGVVPGGGVALIRAQSSLDGIKLSQEQAFGVSIVRRAIEEPLRQIANNAGVDG
                     SIVVSKVKEAQGAFGFNAATLEYGDLVKAGVIDPTKVVRTALQNAASVAALLLTTEAM
                     IAERPKKEAPAPAPGGGGGMGGMGGMGGMDF"
BASE COUNT          394 a         1107 c         1085 g          468 t
ORIGIN      
        1 ggtgctttgc cccccgcccc gccggcggag ccgtcagggt ccccaccccc catctcgctt
       61 cgctcgattg gccacgatga ccagtcgctg gccacgctgc gggccagcgc gtcgcacgtg
      121 gcgaggcgag agcgtcagcg ctggtagagc acaccggcgt agaccagctc ccggccaaac
      181 gatgcgccgt cggtcccgaa gtcgcgccag agcgccccgc gcgcctcgac cgaccaggcg
      241 gtcgacaggg cgcgggtgac caggacctgc agcgagctcc ggttctcgtc ctccaggctg
      301 gtcagctcct ggtggaccag attcgggacg ccttcggggt actggtcgat ctgcagcgtc
      361 gcggtggcgc tgatgaacag cgcgctcacc acctccatcg tcgccgccgc ggtgatccgc
      421 tggcggatca gcgactggcc gtagctgttc gaatcgacga ccgtgacctg gtaggtgccc
      481 gtggcgacca cgtcgccggt ccaggtcagc tccaggccgg cgcgctggta gcgatcgcgg
      541 cgctgcagcg tggtgtccat cggacacggg ttgcccgagt cgaccggcgc gcagttgaac
      601 gtcgccgtgc tgtcgtagct ccgcgcctcg aagcccagcg cgacggcgag ctccaggctg
      661 cgggtgcggt ccggcgtgtg ccacagcatg gcgtcgagcc gcgcgttggc gaccggcccg
      721 cgccagtcga agatgtgcgg cgggagatcg ggcccgtgcg gcttgtacca gaagtcgcgg
      781 ccgccgaccc cgagcgtcag gtggtgatcc tcgtcgtgac cgagcacgag cagcgcgtcg
      841 ccgccgaggt tgcggtaggt ccgggcgccg atcgggagca gcacgccgaa cgaatcggcc
      901 gcggtgaggt ggacgccggc ggcgagcgag ccctcgccga tcggatgcag ccagcgcagc
      961 tcgccgacgt acagcatcac gctctcgtcc cggatctggg ggttcgagct cgcgaccatc
     1021 cgggcgaacc cggacacgtc cacgatgtag gcgccgccga gcaccgggcc gcggctggtg
     1081 atccgcgctc cgagccggcc gaccgcggcg gcgatcggtg cgtcctcggt gcgcgtgacg
     1141 ttggagtcgg cctcggctcc ggactccatc cggaccgtcc acggatccgc ggcggcgatc
     1201 cgggccaccc cgaggatccc gatcgccggc agcgccgcgc gacgcatgac cgcagcctac
     1261 gcgaagccgt cgcggcccgc catcgttcat ggtagccacg ccaggtgcgc tggatcatgt
     1321 cttcgagccg gaccgtcggg cggatgatcg cgtcgatctt cgcgtcgatc ttcgttctgg
     1381 gcgtcggcct ggccgctggc gcgggctgcc gggtcgacgc gcagcagtgc gaccaggcct
     1441 gccgcaacgc catgagcctg gcctactgga aggccgcgga tcaggagatc gccgcggcgg
     1501 cggccgacca gcgcgacgcg ctgcgcaagc agaaggcgag cgaattcacc cagaaggtcg
     1561 aggccggcat cgacggctgc gtcctgcgct gccagtcggc gcgcaaccag gacgccgtgg
     1621 attgtctgat cgccgcgcgg accgccgacc aggcccaggc gtgcaccagg tagcgcgggc
     1681 cgcgccggtg ctacagtagc cggatgtcca gccagcctcc ggggctgctg ttccaccgcc
     1741 gggtcgacca gctcgccgcg cgcgtgcgct ggctcgatcg ctatcgccgg ttcatggcga
     1801 tctcggcggc cgcgctgatc gcgccggtcc tgatcgcgca ggtcgccggc gtgctcggcg
     1861 ccgactggcc ccagctccac gccaccgtgc tgtcggtgat gctcggcgtg atcgtgtggt
     1921 gggtcatcga ggttggcctg gtctacgtca ccgcgctgtg ggagaccgag cacgaccgcc
     1981 tgctccgcga ccgcggcctg ccccgcgcgg tgctgcgccg gccgcggatc cgccgaagcc
     2041 ggagctgaag cgcaagccgg tcgatcgaat cccgggactg cagcggcagc cggagccgcc
     2101 gctgccgcgg ggtcggggga gccggctgga ctagaagtcc atgccgccca taccgcccat
     2161 gccacccatg ccgccgccac cgccgggggc gggcgccggg gcttccttct tcgggcgctc
     2221 ggcgatcatc gcctcggtgg tcagcagcag cgccgcgacc gacgcggcgt tctgcagcgc
     2281 ggtgcgcacg accttggtcg ggtcgatcac gccggccttg accaggtcac cgtactccag
     2341 ggtcgcggcg ttgaagccga acgcgccctg ggcctccttg accttgctga cgacgatcga
     2401 gccgtcgacg ccggcgttgt tggcgatctg gcgcagcggc tcctcgatcg cgcggcgcac
     2461 gatcgacacg ccgaacgcct gctcctggct gagcttgatg ccgtcgagcg aggactgggc
     2521 gcggatcagc gcgacgccgc cgcccgggac cacgccttcc tcgaccgccg cgcgcgtggc
     2581 gtgcagcgcg tcctcgacgc gggccttctt ctccttcatc tcgacctcgg tcgcggcgcc
     2641 gacccggatg atcgcgacgc cgccgaccag cttggcgagc cgctcctgca gcttctcgcg
     2701 gtcgtagtcc gaggtggtct cctcgatctg cttgcgtatc gtcttgaccc gggcctcgat
     2761 gtcgctcttc ttgcccgcgc cgtcgacgac ggtggtgttg tccttgtcga ccgtgatccg
     2821 cttggcctgg ccgaggtcct gcagcgtcag gttctcgagc ttgaggccga ggtcctcggt
     2881 gaccgcctgg ccgccggtca gcaccgcgag gtccttcagc atctccttgc ggcgatcgcc
     2941 gaacccgggc gccttgaccg cgcagacgtg cagcgtgccg cgcagcttgt tgaccaccag
     3001 ggtggcgagc gcctcgccgt cgacgtcctc ggcgacgatc agcagcggct tgcc
//
LOCUS       DASVOR010000260         2881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148386,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000260 DASVOR010000000
VERSION     DASVOR010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148386"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(409..1275)
                     /locus_tag="VF516_05555"
     CDS             complement(409..1275)
                     /locus_tag="VF516_05555"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8107172.1"
                     /translation="MGDLLHEQNRKSWNHATIAHNSHKGDQAAFLRGGGSTLFPEELA
                     LLGDLVGQRLLHLQCNAGQDTLSLVARGAIATGVDISDEAIAFANRLSMDSGLPATFD
                     RADVYDWLESAPRAAFDRVFSSYGAIAWLSDLPTWARGIAQVLAPGGRLVVLEFHPEL
                     FLLAETVDALGLAAGPTVGRIDCPEGVGDYVADSREGLLHGAPYQEGVVEFQNPESSH
                     EFGHTLGSIIDAIARAGLVIEQLSEYPYSNGCKFFTAMIDLGQRRWALPPGAPSVPLM
                     FGLCARRPVVPR"
     gene            complement(1360..2667)
                     /locus_tag="VF516_05560"
     CDS             complement(1360..2667)
                     /locus_tag="VF516_05560"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8107173.1"
                     /translation="MSGARRRIQPEHPFSADLDRRVSIRRAPARRGIMTTPITTHDGS
                     TARRSRIHDALAGHVARGEVPGLIALVARRGVAEVDVIGATAAGGSEPLRRDSIFRIS
                     SMTKPITAAAAMAAIDDGQLTLDEPVDRLLPELAGRRVLRRIDGPLDDTVPASRPITV
                     RDLLTFTLGMGIPFAPPGSMPIQRALDELRLGQGVPQPQAAPALDEWLRRLGTLPLMH
                     QPGERWMYNTGSDVLGALIARASGRPLDQLLRERIFEPLGMTDTGFSVSPAQLDRLVT
                     SYAVDPATGALRRFDPPDGQWSRPPAFPAGSAGLVSTADDFLAFAEMLRNGGSHRGTR
                     VLSAAAVAAMTRDQLSPAQKAASQWVPGFFDRFGWGLGMAVVTGQDDSAAPGSYGWDG
                     GLGTVWRTDPRAEVVTILLTQRAWVRPEPPPVCRDFWRAARAA"
     gene            2697..>2881
                     /locus_tag="VF516_05565"
     CDS             2697..>2881
                     /locus_tag="VF516_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107174.1"
                     /translation="MPNPEKLSEPRKQASPRPASPQQASIERGGLGQSGYTAGRREGD
                     LSLEDVVDTRYVAPPGS"
BASE COUNT          458 a          964 c         1050 g          409 t
ORIGIN      
        1 ccgcgcttct tccacagctc cagctgatcg ggctcggggc gccgccgcct cggttgcgcg
       61 ttgcgttgct cggggggcat gaaagcctga ttagcttctg agcttgagtc aggcaatcga
      121 tttcgccgat atatattcag aaaattatct gttatttgtg aatatgacat gatctcagtg
      181 ttcgggagcc gccccaccca gagatgacac gagtatcagg aagcagcacc acgtggacgc
      241 cgacgccgtg ttgcggcttg tcagtgcatg ggtcgcagcc acggagaagc agcagcacca
      301 cgcgtcagca agcatgtgtg gtggcgtcgt ccgtcaaggc gtttcgacgc gagacgttcg
      361 gtcctgagcc gagcatcttg cgaggcgaac gcttcgccat cccgacgatc agcgtggcac
      421 gaccggtctg cgagcgcaca gcccgaacat gagcggtacg ctcggcgcgc cgggaggtaa
      481 ggcccagcgg cgctggccga gatcgatcat cgctgtgaag aacttgcagc cgttcgagta
      541 cggatactcg gacagctgct cgatgacgag gcccgctcga gcgatcgcgt cgatgatcga
      601 gcccagcgtg tggccgaact cgtgggagct ctccgggttt tgaaactcga cgacgccttc
      661 ctggtagggc gcgccgtgca gcaagccttc gcgcgagtcc gcgacatagt caccgacgcc
      721 ctcgggacag tcgatccggc cgacggtggg gccggcggcg aggccgagcg cgtcgacggt
      781 ctctgccagc aagaacagct cgggatgaaa ctccagcacc accagccgtc caccaggcgc
      841 caggacctgt gcgatcccgc gcgcccaggt gggaagatca gacagccagg cgatcgcgcc
      901 gtaggacgag aacacccgat cgaatgcggc gcggggcgct gactcgagcc agtcgtagac
      961 atcggcgcga tcgaaggtcg cgggaaggcc gctgtccatc gacagacggt tcgcaaacgc
     1021 gattgcctcg tcgctgatgt caacgcccgt ggcgatggcg cctcgtgcga cgagcgacag
     1081 cgtgtcctgt cccgcgttgc actggaggtg gagcaggcgc tggccgacga gatctccgag
     1141 cagcgcgagc tcctccggga acaaggtcga gccaccgccg cggaggaatg cggcctggtc
     1201 tcccttgtgg ctgttgtgtg cgatggtggc gtggttccag ctcttgcgat tttgctcgtg
     1261 gagaagatcg cccacgaagg cattgtagac cctgcgtggt cggcgacccg caggcgtgac
     1321 ccggaacatg agccggtggg gccgggagct gagctgccat caggccgcgc gcgcggcgcg
     1381 ccagaagtcg cggcacaccg gcggcggctc cggccggacc cacgcgcgct gggtcagcag
     1441 gatcgtcacg acctcggcgc gcggatcggt gcgccacacc gtgcccaggc cgccgtccca
     1501 gccgtagctg cccggggcgg ccgagtcgtc ctggccggtc acgaccgcca tcccgaggcc
     1561 ccagccgaag cggtcgaaga agccgggcac ccactgcgag gcggccttct gcgccggcga
     1621 cagctgatcg cgcgtcatcg ccgcgaccgc tgcggccgac agcacgcggg tcccgcggtg
     1681 gctgccgccg ttgcgcagca tctcggcgaa cgcgaggaag tcgtcggcgg tcgacaccag
     1741 cccggcgctg cccgccggga acgccggagg ccggctccac tggccgtcgg gcggatcgaa
     1801 ccgccggagc gcgccggtgg ccggatcgac ggcgtagctc gtgaccagcc ggtcgagctg
     1861 ggccggcgag acgctgaagc cggtgtcggt catgccgagc ggctcgaaga tgcgctcgcg
     1921 caggagctga tcgagcggcc ggcccgacgc ccgcgcgatc agggcgccga gcacgtcgct
     1981 gccggtgttg tacatccagc gctcgccggg ctggtgcatc agcgggagcg tgccgagccg
     2041 gcgcagccac tcgtcgagcg ccggcgcggc ctgcggctgc gggacgccct ggccgagccg
     2101 gagctcgtcg agcgcgcgct ggatcggcat gctgccgggc ggggcgaacg ggatgcccat
     2161 ccccagggtg aaggtcagga gatcgcggac ggtgatcggc cggctggccg gcacggtgtc
     2221 gtcgagcggg ccgtcgatcc gccgcagcac gcgacggccg gcgagctcgg gcagcaggcg
     2281 gtcgaccggc tcgtcgaggg tcagctggcc gtcgtcgatc gcggccatcg ccgcggcggc
     2341 ggtgatcggc ttggtcatcg acgagatccg gaagatcgag tcgcggcgca gcggctcgct
     2401 gccaccagcg gcggtcgcgc cgatcacgtc gacctcggcg acgccgcgcc gggcgaccag
     2461 cgcgatcagg ccggggacct cgccgcgggc gacgtggccc gccagggcgt cgtggatgcg
     2521 gctgcgccgc gccgtcgaac catcgtgggt cgtgatcggg gtcgtcatga tccctcgacg
     2581 agcgggcgct cgccggatcg acacgcgccg atcgagatcg gcgctgaacg gatgctcagg
     2641 ctggatccgg cgccgggcac cggacatgca cctctcgtag atccgaggtg cacgtcatgc
     2701 cgaacccgga gaagctctca gagccacgca agcaggcgtc gcccagaccg gcgtcgcccc
     2761 agcaggcgtc gatcgagcgc ggcggcctcg gtcagagcgg ttacaccgcc ggccgccgcg
     2821 agggcgacct ctcgctcgag gacgtcgtcg atacgcggta cgtcgcgccg ccgggcagca
     2881 g
//
LOCUS       DASVOR010000261         6032 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_2474,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000261 DASVOR010000000
VERSION     DASVOR010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6032
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2474"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2036)
                     /locus_tag="VF516_05570"
     CDS             complement(<1..2036)
                     /locus_tag="VF516_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX8107175.1"
                     /translation="MVRFLRSSPWMPAQDETPWHALDVVSAVERLATDPQRGLDVSAL
                     GELRMRYGPNELPAARQRPLWMVFLNQFRSPLIYLLFGAAAVALLLRETKDAIVIFIV
                     VLLNALIGAYQEGRAEQALAALRRLASQKARVIRGGREEMIDARDLVPGDLLVLVAGD
                     AVTADARVTDHASLQIAEAALTGESVPVSKTTGVIAPESALADRTNMVYAGTHVTAGR
                     ARAIVVATGTATEIGHIAALAEGGTPHKTPLERRVETFGRYLMVVALITFAVFIAVGM
                     AARIRLADIVMVGISQVVGLIPEGLPVAMTVALAIGVQRMARRRAIVRRLSAVETLGS
                     TTVICSDKTGTLTRNEMTVTALWLPGRGLLSVTGGGYAPEGTLLQAGAEVDLEAADDV
                     RRFAEACVLCNDAQVVGPDDRDSRWRAVGDPTEAALVTFAMKVGLVPDAVREGQPRRA
                     EIPFDPAIKLMATQHDTAAGTRIVLKGAAEAILPLCALDEAIRTEAQAMVETMAAQAL
                     RVLAVAELLGAQIDGRLGAAAFRGRARLLGLVGQIDPPRSEAATAVATCLAAGIRPVM
                     VTGDHKVTGLAVARTLGIARGGDIAMDGVELEGMDDAALASGIDRIRVFARVHPAQKL
                     RIVEAYQRRGEVVAMTGDGVNDAPALVRADVGVAMGITGTDVAKEAAKIVVTDD"
     gene            2318..4012
                     /locus_tag="VF516_05575"
     CDS             2318..4012
                     /locus_tag="VF516_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8107176.1"
                     /translation="MHLPSVVAIIALLAGCHPKRLEKVGELELHGEHVELRKRAKGSP
                     PNDPDTPAMLVLALDGVSRQLLYDMLHRGELPNLAMLLGGEHFDHAYFDHSLLSTLPS
                     TTMAAWATAFTGVPPGQHGVTGNEYFVRETRTFVCPAPVSFADATPTLEIYTDDSLGK
                     VIDAPTVYERLREQAPDALVWVAMNHVYRGADLLLLARRTAIVGAIDGYLESFAAEHL
                     TDSSSRKLYENLDNSALGSVLARLDKGPVPDVLTFYVSGTDLYAHTAKEGPDPARRTY
                     LAEVVEPAIGQLLDKLRARDAGANRWIIVTADHGHTEVIKDRQHALATAPADVLRGAG
                     FRPRPFKRTVDKSDPFSAVLAYGGAMAYVYLADRSKCPGDHDVCAWDQPPRYAEDVLA
                     AAEAFARANAGDPAVPAMTGTLDMILVRRPRPVSDIDLPFEVYVGGGRTVAIDDYLAG
                     HPHPSYVAFAARMHDLAVGPHGEHAGDLLLLAHNGDRSDPEQRFYFASPYRSWHGSPS
                     RADSELPFIVAHPMLRAAAIGARVRRVLGGRPFQQKVTDVILELRRSPAPRPAEAH"
     gene            complement(4056..>6032)
                     /locus_tag="VF516_05580"
     CDS             complement(4056..>6032)
                     /locus_tag="VF516_05580"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEX8107177.1"
                     /translation="ALVVFPRAVQRAPERSPVDRPMARRAVTRLALTGFLGIGYEVLV
                     VRVLSQVAEDTVYTFALLLAVYLVGTAAGAAGYQRWLACDRGRDHLGDRLLAALASAC
                     LVGTASLWAAERVKAWALGVLGAGYATALAAEALLAVLAFGLPTIAMGAVFSHLSRSA
                     SQAGVGFGRAVGVNLLAAAAAPAVCGVVAIPALGPKAVLLLIAAGYLAAATRRAWTRP
                     ALWVPAMAALALAVVAPPLRFVELPAGGRVVRYRDGVMAAVSVVEDADGVARLRIDNR
                     QQEGSSATRRVDSRQAWLPLLLHPAPRRALFLGLGTGVTASSAAEDPTLDVDVVELLP
                     EVIAASTHFVRSDARSRLHVIAADARRYVRASDRRYDVIVSDNFHPARSGSAALYTVE
                     HFEAVRRRLDPGGVFCQWLPVHQLDLGTLRSIARSFVTVYPTSWAVIASNSLETPVLG
                     LVARRDQGRFGLPRLVERLARLAPRDRLTELGLEDELAVLGSAIAGPAALSRFAAGAR
                     TNTDDHPIVAYTAPRATYAPDSAPRDRLIALLRELSIEPDELVLPVADPAWRDRLAAY
                     WAARNRFVESGRDVRPSPSVQDMLAQVREPLLSVLRISPDFRPAYDPLLAMAMALARS
                     DVSGARALLGELSRLQPARSEAIQLLAGLGPEPR"
BASE COUNT          954 a         2224 c         2037 g          817 t
ORIGIN      
        1 ttgtcatcgg tgacgacgat cttggcggcc tccttggcga cgtcggtacc ggtgatcccc
       61 atggcgacgc cgacatccgc gcggaccagg gcaggcgcat cgttgacgcc gtctccggtc
      121 atcgcgacga cctcaccgcg cctctggtat gcctcgacga tccggagctt ctgggccggg
      181 tggacgcggg cgaatacccg gatgcgatcg atcccggacg cgagcgccgc atcgtccatg
      241 ccctcgagct cgaccccgtc catggcaata tcgccgcctc gcgcgatgcc gagagtgcgc
      301 gcgaccgcga gaccggtcac cttgtggtct ccggtcacca tgaccgggcg gatcccggcc
      361 gcaaggcagg tcgccaccgc ggtagcggcc tcgctgcgcg gcggatcgat ctgacctacg
      421 agaccgagca gcctggcacg tccacggaag gccgcggctc cgagccgtcc gtcgatctgt
      481 gcaccgagca actcggcaac ggcgaggacg cgcagcgcct gcgccgccat cgtctcgacc
      541 atcgcctgcg cctcggtgcg tatcgcctcg tccagcgcgc acagcggcag gatggcctcg
      601 gcggcgccct tgagcacgat acgggtaccc gcggccgtgt cgtgctgcgt cgccatcagc
      661 ttgatcgccg gatcgaacgg gatctccgcc cgccgcggct ggccttcgcg caccgcatcc
      721 ggaacgagcc caaccttcat tgcgaaggta acgagcgctg cctcggttgg atcgccgacc
      781 gcccgccacc gcgaatcgcg gtcatcaggt cccaccacct gtgcgtcgtt gcaaagcacg
      841 caagcctcgg cgaaccggcg aacatcgtcc gccgcttcca ggtcgacctc ggcgccggcc
      901 tgcagcagcg ttccttcggg cgcgtagccc ccaccggtga cggacagcag gccgcggccg
      961 gggagccata gcgcggtgac ggtcatctcg ttgcgggtca gcgttccggt cttgtcgctg
     1021 cagatcaccg tggtcgagcc gagggtctcg accgccgata gccggcgcac gatcgcgcgc
     1081 cgcctcgcca tccgctgcac gccgatggcg agcgccacgg tcatcgcgac gggtagtccc
     1141 tcggggatca ggccgacgac ctggctgatc ccgaccatca cgatgtcggc tagccgaatt
     1201 cgcgccgcca tcccgacggc gatgaacacg gcgaacgtga tgagcgcgac gaccatcagg
     1261 taccggccaa acgtctcgac ccgacgctcg aggggcgtct tgtgcggagt accgccttcc
     1321 gcgagcgccg cgatgtgacc gatctcggtc gctgtaccgg tcgcgaccac gatcgcgcgc
     1381 gcccggccgg cggtcacgtg tgtcccagcg taaaccatgt tcgtgcgatc ggcgagcgcc
     1441 gactcgggcg cgatcacgcc ggtggtcttc gagaccggca ccgattcacc ggtcagcgcc
     1501 gcctccgcga tctgtagtga cgcatggtcc gtcacgcgcg catccgcggt gacggcgtcc
     1561 ccggcgacga gcaccagcag atccccagga acgaggtccc gcgcgtcgat catctcctcc
     1621 cggccaccac gtatcacgcg cgctttctgg gaggccaggc gccgcagcgc agcaagcgcc
     1681 tgctctgcgc ggccttcctg gtacgcgccg atcagcgcat tcagcaggac cacgatgaag
     1741 atgacgatcg cgtccttggt ctcgcgcagc agaagtgcga ccgcagcagc cccgaatagc
     1801 aggtagatca gcgggctccg gaactggttc aggaacacca tccacagagg acgctggcgg
     1861 gcggccggca gctcgttcgg cccgtaccgc atacgaagct cgccgagtgc gctcacgtcc
     1921 aggccgcgtt gcggatccgt ggcgagccgc tcgacggctg aaaccacgtc gagcgcatgc
     1981 catggagtct cgtcctgcgc cggcatccat ggtgacgatc gcaggaaccg gaccacgctc
     2041 gacccatcat tctgccgtgc agtggacgca gagcttgcac gcaagccagg ttgccgcgca
     2101 ctcgttgcgc gtctgcgagg agtccacgac catacgacgg acgccctggt gcccagcctg
     2161 ccagcgtacg cgagcgtcgt cgggtcgccg aaggccactc cgtgcacccg cggccatgcc
     2221 gcatgtccaa gttgcccctg acgtaggaac gcaggcgcgt ggctgccggc ccgctcggcg
     2281 cacccgccgg cgagcggttc gtgcgacgct cttgatcgtg cacctaccaa gcgtcgtggc
     2341 gatcatcgcg ctcctcgccg gctgccaccc gaagcggctg gagaaggtcg gagagctcga
     2401 gctgcacggc gagcacgtcg agctgcgcaa gcgggcaaag ggctcgcccc cgaacgatcc
     2461 ggatacgccg gccatgctgg tcctcgcact cgacggagtc tcgcgccagc tgctctacga
     2521 catgctgcat cgcggcgagc tgcccaatct cgcgatgctg ctcggcggcg agcacttcga
     2581 tcacgcgtac ttcgaccatt cactgctgtc gacgctaccg tcgacgacga tggcggcgtg
     2641 ggccaccgcg ttcaccggcg tccccccggg gcagcacggc gtgacgggca acgagtactt
     2701 cgtgcgcgag acccggacgt tcgtgtgtcc cgcgccggtg tcgttcgccg atgccacccc
     2761 gaccctcgag atctacaccg acgacagcct cggcaaggtg atcgacgctc cgaccgtgta
     2821 cgagcgcttg cgtgagcagg cgcccgacgc gctcgtctgg gtcgcgatga accacgtcta
     2881 ccgcggcgcg gatctgttgc tgctcgccag gcgaacggcg atcgtcggcg cgatcgatgg
     2941 ctacctcgag tcgttcgccg ccgagcacct gaccgacagc tcgtcgcgca agctctacga
     3001 gaacctcgac aactcggcgc tgggctccgt gctcgcgcgg ctcgacaagg gcccggtgcc
     3061 cgacgtcttg acgttctatg tcagcggcac cgacctgtac gctcacacgg ccaaggaggg
     3121 gcccgacccc gcgcgacgca cctacctcgc cgaggtggtc gagcccgcga tcggccagct
     3181 gctcgacaag ctccgcgcgc gcgacgcggg cgcgaaccgc tggatcatcg tcaccgcaga
     3241 tcatggccat accgaggtga tcaaggaccg gcagcacgcg ctcgccaccg cgcccgcgga
     3301 cgtgctgcgc ggcgccgggt ttcgccctcg cccgttcaag cgcaccgtcg acaagtccga
     3361 tccgttcagc gcggtgctcg cgtacggcgg cgcgatggcc tacgtctacc tcgccgatcg
     3421 ctcgaagtgc cccggcgatc acgacgtctg cgcgtgggac cagccgccgc gctacgcgga
     3481 agacgtgctc gccgcggccg aggcgttcgc gcgcgccaac gccggcgatc cggccgtgcc
     3541 ggcgatgacc ggcacactcg atatgatcct ggtgcggcgg ccgcgcccgg tgagcgatat
     3601 cgatcttccg ttcgaggtct acgtcggcgg tggaaggacc gtggcgatcg acgactacct
     3661 ggctggccac ccgcatccga gctacgtcgc gttcgccgcg cgcatgcacg acctcgcggt
     3721 cgggccccac ggcgaacacg ccggcgatct gttgttgctc gcccacaacg gcgaccgcag
     3781 cgatccggaa cagcggttct acttcgcatc gccgtaccgc tcgtggcacg gcagcccgtc
     3841 gcgcgccgac tccgagctgc cgttcatcgt cgctcacccg atgctgcgcg ccgcggcgat
     3901 cggcgcgcgg gttcgccggg tgctcggcgg ccgcccgttt cagcagaagg tcaccgacgt
     3961 gatcctcgag ctgcgccggt cacccgcgcc gcgacctgcc gaggcacact gaggcggcaa
     4021 ctggcggacc gcaccgcccg cgcatggaaa ggcgatcacc gaggctccgg gccgaggcca
     4081 gcgagcagtt ggatcgcctc ggagcgcgcc ggctgcagcc ggctcagctc gccgagcagg
     4141 gcacgcgcgc cggacacatc cgaccgggca agcgccatcg ccatcgcgag caacggatcg
     4201 taggcgggcc gaaaatccgg gctgatccgc agcacggaca gcagcggctc gcgcacctgc
     4261 gcgagcatgt cctgcacgct cggcgagggc cggacgtccc ggcccgactc gacgaaccgg
     4321 ttgcgagcag cccagtaggc ggccaggcga tctcgccacg ccggatcggc gaccggaagc
     4381 acgagctcgt cgggctcgat cgagagctcg cgcaacagcg cgatcagccg gtcgcgcggg
     4441 gccgaatcgg gcgcgtaggt cgcgcgcggg gcggtgtacg ccacgatcgg atgatcgtcg
     4501 gtattcgtgc gggcgcccgc ggcgaaccgc gacagggccg cggggcctgc gatcgcgctg
     4561 ccgagcaccg ccagctcgtc ttccagcccg agctcggtga gccggtcccg gggcgccagg
     4621 cgcgcgagac gctcgacgag ccggggcaga ccgaaccgac cctgatcgcg ccgcgcgacg
     4681 agaccgagca cgggggtctc gaggctgttg ctcgcgatca ccgcccagct cgtcgggtac
     4741 acggtcacga acgagcgcgc gatgctgcgc agggttccca gatcgagctg atgaaccgga
     4801 agccactggc agaacacgcc gcccggatcg agccgccggc ggacggcctc gaagtgctcc
     4861 accgtgtaca gcgccgccga tccgctcctc gccggatgaa agttgtccga cacgatgacg
     4921 tcgtatcgtc gatcgctcgc gcgcacgtag cggcgcgcat cggcggcgat cacgtgaagc
     4981 cgggatcgcg cgtcactccg cacgaagtgg gtcgatgccg cgatcacctc cggcaacagc
     5041 tcgaccacgt cgacgtcgag tgtcgggtcc tccgcagcgg acgacgcggt gaccccggtc
     5101 ccgaggccga ggaacagcgc acggcgcggc gcggggtgga ggagcaaggg cagccacgct
     5161 tgccggctat ccacgcgacg cgtcgcgctg ctaccttcct gctgccggtt gtcgatgcgc
     5221 aggcgcgcga ccccgtcggc gtcctcgacc acgctcacgg cggccatcac gccgtcccgg
     5281 tagcggacga cccgaccgcc ggcaggtagc tcgacgaacc gcagcggcgg cgcgacgacc
     5341 gcgagcgcca gcgcggccat cgccggcacc cagagcgcgg gccgggtcca cgcgcgacgg
     5401 gtggccgccg caagataacc cgctgcgatc agcagcagca cggccttcgg tccgagcgcg
     5461 gggatggcga cgacgccgca caccgccggc gccgcggccg cggcgagcag gttcacaccg
     5521 acggcgcgac cgaagccgac gccggcctgg ctcgccgagc ggctcaggtg gctgaacacg
     5581 gcgcccatcg cgatcgtcgg cagcccgaag gccagcaccg cgagcagcgc ctcggccgcg
     5641 agcgcggtcg cgtagccggc tccgagcacg cccagcgccc aggccttgac gcgctccgct
     5701 gcccacagcg acgccgtccc cacgagacag gcggacgcca gcgcggcgag cagccgatcg
     5761 ccgaggtggt cgcggccgcg atcgcatgcg agccatcgct ggtaaccggc tgcacccgcc
     5821 gcggtgccca cgagatacac cgcgagcagc agcgcgaacg tgtagaccgt atcctccgcc
     5881 acctggctga ggacgcgaac gacgagcacc tcgtagccga tgcccagaaa gccggtgagc
     5941 gcgagccgcg tcaccgcgcg gcgcgccatg ggtcggtcca ccggagaccg ctccggtgcg
     6001 cgctggaccg ctcgcggaaa cacgaccagc gc
//
LOCUS       DASVOR010000262         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265275,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000262 DASVOR010000000
VERSION     DASVOR010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265275"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..821
                     /locus_tag="VF516_05585"
     CDS             111..821
                     /locus_tag="VF516_05585"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8107178.1"
                     /translation="MEVSEQSYRELAADTAHEIRTLLTPFEGYLVALRRHLAAQGRVD
                     DRAEHYLTAALARLQQIQAFTEDLCTYSSAAEAGFAAVSVAEVVNDAVEISFDHMANR
                     SGLPDIVREIEVSPGLVVEAMPERLTRAVANVVTNALQAMPDGGRLSIAARHVGHDVE
                     LTVADTGCGMSPEVVEQARLRFRTTRRDQGGTGLGLPISDRIVVGDHGGELIIDSRVG
                     EGTTVTIRLPVRQRTGER"
     gene            844..1644
                     /locus_tag="VF516_05590"
     CDS             844..1644
                     /locus_tag="VF516_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107179.1"
                     /translation="MIVEDDRAIRELLADRLRPYELTFSVCQLECFERLDAAEFDLVL
                     LDLRLPRTSSDMAASNEVGLDILRQIRKRGLRKRGSTMVLPVVVMTAYGSEAITAQVL
                     VECGANDYIPKPFRQGDDLERKIERALHGAGALVPAANASTNTICVYFDPKERALRIE
                     SIPYADHYDLMKVLIEAHARDLGDLRAWEDYAAIPGRDLAQRLNIGEKALRQRVSRFR
                     ETVTSDLREQLGRVVGPNDIIENQRRWKGYRLNPRTVRVFRHPASGAR"
BASE COUNT          316 a          599 c          648 g          299 t
ORIGIN      
        1 tctggacgct gaccgcgccc gtacaggatc cgatcctcaa gcacatcgcg aagcggaccc
       61 gcgagatcgg cctgcggtcg ctgacgcccg cgctgatcta cgatatcgcg atggaggtca
      121 gcgagcagtc gtaccgcgag ctggccgcgg acaccgcgca cgagatccgg accctgctga
      181 cgcccttcga gggatatctc gtggcgctga gacgtcacct cgccgcccag gggcgagtcg
      241 acgacagggc ggaacactac ctcacggcgg cgctggcgcg cctccagcag attcaagcgt
      301 tcaccgagga cctttgcacc tacagctccg ccgcggaggc cggcttcgct gcggtatccg
      361 tggccgaggt ggtgaacgac gcggtcgaga tcagcttcga ccacatggcg aatcgctccg
      421 ggcttccgga catcgttcgc gagatcgagg tgtcgccggg gctcgtggtc gaggccatgc
      481 cagagcggtt gacgcgcgcg gtcgcgaacg tggtcacgaa tgctctgcaa gcgatgccgg
      541 acggtggcag actttccatc gcggcgaggc acgtgggcca cgacgtcgag ctcaccgtgg
      601 cggacaccgg gtgcggaatg tcaccggagg tcgtcgagca ggcgcgtctg cgattccgga
      661 ccactcgacg agatcagggc ggaacggggc tgggcctacc gatctcggac cgcatcgtgg
      721 tgggcgacca tggcggtgag ctgatcatcg actcgcgggt cggggagggt accacggtca
      781 ccatcagact acccgtgagg caacgaaccg gtgagcgctg atgagcccag gaacctgcgg
      841 ctgttgatcg tcgaggacga cagggcgatc cgcgagctgc ttgccgacag gctgcgtccc
      901 tacgagctga cattcagcgt ctgccagctg gaatgcttcg agcggctcga cgccgccgag
      961 ttcgacctcg tcctcctgga tctccggctc ccgaggacgt cgagcgacat ggccgccagc
     1021 aacgaggtcg gcctcgacat tcttcggcag atccggaagc gcggtctgcg aaaacgtgga
     1081 tccacgatgg tgctgccggt ggtcgtgatg acggcgtacg ggtccgaggc gatcacggcc
     1141 caggtcctcg tcgaatgcgg tgcgaacgac tacattccaa agccgtttcg acagggcgat
     1201 gacctcgagc gcaagatcga gcgcgcgctc cacggcgccg gcgcgctggt tccggccgcg
     1261 aacgcttcca cgaacacgat ctgcgtctac ttcgatccga aggaacgagc cttgcgcatc
     1321 gaatcgattc cctatgccga tcactacgac ttgatgaagg tgctgatcga ggcccacgcc
     1381 agggatctgg gcgatctgcg ggcatgggaa gactacgccg cgattcccgg ccgggacctg
     1441 gcccagagac tgaacatcgg agagaaggcg ctgcgtcagc gggtctcccg atttcgcgag
     1501 acggtcacga gcgatcttcg cgaacagctc ggtcgcgtcg tcggtccgaa cgacatcatc
     1561 gagaaccagc ggcgctggaa gggatatcga ctgaatccgc ggaccgttcg cgtgttccgg
     1621 caccctgctt ccggcgcccg gtgaacggcg cgcgttgccg cccaccgcgg ctcttcccgg
     1681 ggttcgagga cgcgtcgagc gttcgcggcc gcggcatgga acaatcgggg cgtgcgcgcg
     1741 ttgctgatct gtgccgcgct ggccgcctgt ggccggcccg agagggcacc cggtgcgccg
     1801 gcgcccggag ctgtgctcgc cggtgcgggt ggggccgccg atgccgggcg cgaatcgtct
     1861 gg
//
LOCUS       DASVOR010000263         8015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_177824,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000263 DASVOR010000000
VERSION     DASVOR010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177824"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..1847
                     /locus_tag="VF516_05595"
     CDS             360..1847
                     /locus_tag="VF516_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107180.1"
                     /translation="MPHADVRLERSPLVGARVFDRREAELVWEAMAAVAAHSPADALI
                     LQSLIAELKATSDLLERQRPLRWPISLAGETRDATTLIDHLCTLDGLSGDLVLPLKAS
                     QSRTYLLTKINLLRGFVRATSMLGDAPDSARMAHDLREELAQSIYTLLAEELLLAVLR
                     MPDVGRQTKQRAADQLITVWDDAALEIDDFAPLLESAWYARNRINSAYGNLLGARETF
                     RLVGENCRPEVLEFFGREGMSADEIAAFEEFLFNMTSEELATLRREMQSQRVPAASPA
                     WAAAVLGRQIEELEHAREIDPMALYRSYQRRQLAADFRVKANAPGPRRTAEAYLLCFL
                     LDQGAGPSVRDAEPARSTREWAIQPAGDSDEAFVTRYADISCPARVARSCDRFPLTVA
                     LVPHPPESSAIDASFEVRRGLRMEVRLGALGLHVLGPPQARIFPQGDRCVFFLRPDEL
                     GRHSVTLDFAQDGKALGSVAIQIEVVEHDVPYDATRSPATGPGAR"
     gene            complement(1883..2776)
                     /locus_tag="VF516_05600"
     CDS             complement(1883..2776)
                     /locus_tag="VF516_05600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8107181.1"
                     /translation="MAWTVDQPGATLAAFVKARTGVPWSVAKRQIATGKVFVDGACAT
                     AVDLRLAAGQQVELRAGAPRPRDPMREGVLVHDDAHVVVLDKPSGVNSVPYEDRETGT
                     AMDLVRGAWRRQGKPATQVALHVVHRIDRATSGLLMFAKTKQAELGLAAQLRAHTVTR
                     SYLCVAHGAVAAGRIESYLVRDRGDGLRGSTTRRDQGKRAITHVEIVRPLRGATLCRV
                     RLETGKTHQIRIHLAEAGHPLVGEQVYVRDHRGPVIASPRLLLHAATLGFDHPVTGAR
                     IELSSPLPPDFTAVLDALTPA"
     gene            2862..4007
                     /locus_tag="VF516_05605"
     CDS             2862..4007
                     /locus_tag="VF516_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8107182.1"
                     /translation="MDRTLFSADHDTFRQAFRQFIDREIRPHQERWREQGQVDRETWR
                     KAGAKGFLCPWLDEKHGGPGADFLCSVIVMEELARAYESGFAMSLHSDIVVPYLYSFG
                     TDEQKQRWLAGCASGELVTALGMTEPGTGSDLAAIATTAVRDGDDYVINGQKTFISNG
                     QLCDIVVVAAKTDPDPANAHKGISMFVVEAGRKGFIKGKKLHKMGMASQDTSELFFED
                     CRVPAANRLGPEGGGFMMMMQKLQQERLCVAVGAVAGAEQVLEDTIAYTKERKAFGKP
                     ISKFQNTQFKLVECLAKVEVGRAYVDKVIAGHVAGKYLVKECSIAKFWTTDMGQEVID
                     ACLQLFGGYGYMIEYPVTRAFMDARVARIFAGTNEIMKVIVAKQLGL"
     gene            4328..5248
                     /locus_tag="VF516_05610"
     CDS             4328..5248
                     /locus_tag="VF516_05610"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107183.1"
                     /translation="MPIAVIDIGTNTLLLLIVDQAIHPLVDLCRFGRLGKGLDATGRL
                     AADAIATSLEICREYRQVMDDHGVARPIVVATQAAREAANADDFLGPAEQILGAPIQV
                     IAGEREAELAALAVARTFPELAATRYLVVDVGGGSTEVIAVDAGRVVSQTSVPIGAVR
                     LTERHLHHDPPIVPEIVALSADIDRHLAALDLPRSVPVIGTAGTATTLAALKLGLERY
                     DPEAVTGLRISRDAVNILHARLLALSVAERKTLPGIEPQRADVIAAGAAIFGRILQRI
                     DAPVLIICDRGIRWGLAYEAMSTGGPAGAS"
     gene            complement(5325..5723)
                     /locus_tag="VF516_05615"
     CDS             complement(5325..5723)
                     /locus_tag="VF516_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107184.1"
                     /translation="MGDLLIVDDDVDTAELLGEALALGGHRVRIAHDGMVGLRELSVR
                     MPDAVLLDVEMPNLDGPGMALQMFVRNCGLERIPIILLSGVLDLERVAARVGTPYFLS
                     KPYALGAVRALCDRALAERTPPAPVLDGST"
     gene            complement(5723..7159)
                     /locus_tag="VF516_05620"
     CDS             complement(5723..7159)
                     /locus_tag="VF516_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8107185.1"
                     /translation="MSLRRSFLVFAAVLAVIGTGVAIALILLTTNLRRAAEEVGIEVE
                     SLRTGEELELELIALREETDPLGRAAREGAIRHQLSEAGKYITLRREREILAELTGRV
                     DQYIAALDRAAATGATPAQVRSDTHAAFEPVFALARQWMQLNVEQGRAAERTAARWSQ
                     LADVIGVAAILLLVVGLGGLAWWLQRRTFRPALQLSGAIERYTRGDRSARASEQEPEE
                     FRTIARGFNDMAQTLDRQRADQLAFLAGVAHEIRNPLGALKLATGMIPPDQPLPPEPA
                     VRQLVARIDRQIGRLERMAYDFLDVSRIESGHLELKVEDCDARELVRATLDLFGPAAG
                     THPLAMSAPDEPVRVRCDPMRIEQVLTNLVSNALKYSPPGGSVRVTVAQQPELVAIAV
                     ADEGVGMSADEIEHVFDPFRRGAGATRAIPGAGLGLFVARRIVEAHGGEIAVESVPAA
                     GTTFTVRLPVGASPEPAAAGPRGAAEPA"
     gene            complement(7426..>8015)
                     /locus_tag="VF516_05625"
     CDS             complement(7426..>8015)
                     /locus_tag="VF516_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107186.1"
                     /translation="SRGMREMIELEYGITDRWDVALYNMLDMIRSGDTESGYAGFKVE
                     TRYRPADRGEWFVDPVLYLELQQLFRGDAHQRVEGKLILAKDIGNLNIAGNLALEEER
                     MTDATWNTEVEYAAGTSYALSPAWMIGAEVFGKAEKGEMGGVENRSWAGPALSWAGGG
                     RGVLRGVWVTLAGGAGLTSDSDAYYARLIVGFQFH"
BASE COUNT         1159 a         2910 c         2809 g         1137 t
ORIGIN      
        1 ccgcgcgagc gcccggcgcc gctgcccgcg cgcgtcgagc cgccggcgcc ggtgcaggtg
       61 cccgagccgg tgtcggtgca ggcgcccgag ccggtgtcgg tgcaggcgcc cgagcaaccg
      121 accccgctcg gtgagccgcg tcttctgcgc taaatgctga cggcgctcgt cgggcgccgc
      181 gggagcccgc cgccgctgcc tcttgaacac cccgctcggt gcgggcacaa tccggctgtg
      241 cttgggaggt tgccgggcac tctcgcgtgt cagacttcga tcgcgccggt gtccgtcctc
      301 ccgcaggtgt gcgcaacacg cccgtcgggc gtccaccggg tgtacccgag gtcgaggctg
      361 tgccacacgc cgacgtccgc ctcgaacgca gtccgctggt cggcgcccgc gtgttcgatc
      421 ggcgtgaagc cgagctggtc tgggaggcga tggcggcggt cgccgcacac tcacctgccg
      481 acgcgctgat cctccagagc ctgatcgccg agctcaaggc cacctcggac ctgctcgaac
      541 gccagcgccc gctgcgctgg ccgatctcgc tcgccggcga gacccgcgac gcgaccaccc
      601 tgatcgacca tctgtgcacg ctggatggcc tgtcgggcga cctcgtgctc ccgctcaagg
      661 cgtcgcagtc gcgcacgtac ctcctgacca agatcaactt gctgcgcggc ttcgtgaggg
      721 cgacctcgat gctcggtgac gcgcccgatt cggcgcggat ggcccacgat ctacgagaag
      781 agctcgcgca gtcgatctac accctgctcg ccgaggagct gctgctcgcc gtgctgcgca
      841 tgcccgacgt cggccgccag accaagcagc gcgccgcgga tcagctgatc acggtgtggg
      901 acgacgcggc gctcgagatc gacgacttcg cgccgctgct cgagtcggcg tggtacgccc
      961 gcaaccggat caacagcgcc tacggcaacc tgctcggcgc ccgcgagacg ttccggctgg
     1021 tcggcgagaa ctgccgcccc gaggtcctgg agttcttcgg ccgcgagggc atgagcgccg
     1081 acgagatcgc cgcgttcgag gagttcctgt tcaacatgac cagcgaggag ctggccacgc
     1141 tgcgccgcga gatgcagagc cagcgggtgc cggccgcgag cccggcgtgg gccgccgcgg
     1201 tgctgggccg gcagatcgag gagctggagc acgcccgcga gatcgatccg atggcgctct
     1261 accgcagcta ccagcgccgc cagctcgccg ccgacttccg cgtcaaggcc aacgcccccg
     1321 ggccgcgccg caccgccgag gcctacctgc tgtgcttcct gctcgatcag ggcgccgggc
     1381 cgtcggtccg cgatgcggag ccggcccgct cgaccaggga gtgggcgatc cagccggccg
     1441 gcgatagcga cgaggcgttc gtgacccggt acgccgacat cagctgtccg gcccgggtcg
     1501 cgcgctcgtg cgatcggttc ccgttgaccg tcgcgctcgt gccgcacccg ccggagagca
     1561 gcgcgatcga cgcctcgttc gaggttcgcc gcggcctgcg catggaggtc cggctgggcg
     1621 cgctcggact tcacgtgctc ggtcctcccc aggcacggat cttcccgcag ggcgaccgct
     1681 gcgtgttctt cctgcgcccc gacgagctcg ggcgacacag cgtcacgctc gacttcgcac
     1741 aggacggcaa ggcgctcggc agcgtcgcga tccagatcga ggtcgtcgag cacgacgtcc
     1801 cgtacgacgc cacccggagc ccggccaccg gccccggcgc gcgctgagcc cgatccgggc
     1861 accggccggc cgctcgtcgc cgtcacgcgg gggtcagcgc gtccagcacc gcggtgaagt
     1921 cgggcggcag cggcgacgac agctcgatgc gcgcgccggt caccgggtgg tcgaagccga
     1981 gcgtcgcggc gtgcaggagc aggcgcggcg acgcgatgac cggcccgcgg tggtcgcgga
     2041 cgtagacctg ctcgccgacc agcgggtggc cggcctcggc gaggtggatc cggatctggt
     2101 gggtcttgcc ggtctccagg cggacgcggc acagcgtggc gccgcgcagc ggccgcacga
     2161 tctcgacgtg ggtgatcgcg cgcttgccct gatcgcgccg cgtggtcgag ccgcgcaggc
     2221 cgtcgccgcg gtcgcgcacc aggtacgatt cgattcgccc cgctgcgacc gcgccgtgcg
     2281 cgacgcacag gtagctgcgg gtgacggtgt gggcgcggag ctgcgcggcg aggccgagct
     2341 cggcctgctt ggtcttggcg aacatgagca ggccgctggt cgcgcgatcg atccggtgga
     2401 cgacgtggag cgcgacctgg gtcgccggct tgccctggcg ccgccacgcg ccgcgcacga
     2461 ggtccatcgc cgtcccggtc tcgcggtcct cgtagggcac gctgttgacc ccgctcggct
     2521 tgtcgagcac gaccacgtgg gcgtcgtcgt ggaccagcac gccctcgcgc atcggatcgc
     2581 gcggccgcgg cgcgccggcg cgcagctcga cctgctggcc ggcggcgagc cggaggtcga
     2641 ccgcggtcgc gcacgcgccg tcgacgaaca ccttgccggt ggcgatctgg cgcttggcga
     2701 ccgaccacgg cacgccggtc ctcgccttga cgaacgccgc gagcgtcgcg ccgggctgat
     2761 cgacggtcca tgccatgcgg gcggcagcct agctcaaatg gaccacgtgc cccggcggcg
     2821 attgtcgcgg ccgatcggcg tccgcgtgat atgaccggag gatggatcgc acgctgttct
     2881 cggccgacca cgatacgttc cgtcaggcgt ttcgccagtt catcgaccgc gagatccgcc
     2941 cccaccagga gcgctggcgc gagcagggcc aggtcgaccg cgagacctgg cgcaaggccg
     3001 gcgccaaggg cttcctgtgc ccgtggctcg acgagaagca cggcggaccg ggcgcggact
     3061 tcctgtgctc ggtgatcgtg atggaggagc tggcgcgggc ctacgagagc gggttcgcga
     3121 tgtcgctgca ctccgacatc gtggtcccgt acctctactc gttcggcacc gacgagcaga
     3181 agcagcgctg gctggccggc tgcgcgtccg gcgagctggt caccgcgctc ggcatgaccg
     3241 agcccggcac cggctcggac ctcgcggcga tcgcgaccac cgcggtgcgc gacggcgacg
     3301 actacgtgat caacggccag aagacgttca tctcgaacgg ccagctgtgc gacatcgtcg
     3361 tggtcgcggc caagaccgac cccgatccgg ccaacgcgca caaggggatc tcgatgttcg
     3421 tcgtcgaggc cggccgcaag ggcttcatca agggcaagaa gctgcacaag atggggatgg
     3481 cgtcgcagga cacctccgag ctgttcttcg aggactgccg ggtgccggcc gccaaccggc
     3541 tcggccccga gggcggcggc ttcatgatga tgatgcagaa gctccagcag gagcggctgt
     3601 gcgtcgcggt cggcgcggtc gccggcgccg agcaggtgct ggaggacacc atcgcctaca
     3661 ccaaggagcg caaggcgttc ggcaagccga tcagcaagtt ccagaacacc cagttcaagc
     3721 tggtcgagtg cctggccaag gtcgaggtcg gccgcgccta cgtcgacaag gtgatcgccg
     3781 ggcacgtcgc cggcaagtac ctggtcaagg agtgctcgat cgccaagttc tggaccaccg
     3841 acatgggcca ggaggtgatc gacgcctgcc tgcagctgtt cggcggctac ggctacatga
     3901 tcgagtaccc ggtgacccgc gcgttcatgg atgcccgggt cgcccggatc ttcgccggca
     3961 ccaacgagat catgaaggtg atcgtcgcga agcagctcgg actgtgaccg gcgaggcggc
     4021 gcagcgagac cctcctcacg ggccagcctc gtcgaggatc gcatcaccca tgctgcgagg
     4081 cgtggtggag atgacaactc gcgatctgca attctgaacg aatacacatc cggtgaaatc
     4141 accggttgcg cgtgtgcgat gacagtcacg ctgtcgcgcg cgcgacggcg atcacctcgg
     4201 tgtcggcgct ggcgagcctg cccgcaccgg cgagcgactc cgccgcacac agcgcacacg
     4261 tcaccggtgt tcgatctgcg gaacacatga ctggctcgcc ggaccgcact ggcgttatac
     4321 ccgcagcatg ccgatcgcgg tgatcgatat cgggaccaac acgctgctgc tgttgatcgt
     4381 cgaccaggcg atccacccgc tcgtcgacct gtgccggttc ggccggctgg gcaaggggct
     4441 cgacgcgacc ggccggcttg ccgccgacgc gatcgcgacc agcctggaga tctgccgcga
     4501 gtatcgccag gtgatggacg accacggcgt cgcgcggccg atcgtggtcg cgacccaggc
     4561 ggcgcgcgag gcggccaacg cggacgactt cctcggcccc gccgagcaga tcctcggcgc
     4621 gccgatccag gtgatcgccg gcgagcgcga ggccgagctc gcggcgctgg cggtcgcgcg
     4681 cacgttcccc gagctggcgg cgacgcgcta cctggtggtc gatgtcggcg gcggctcgac
     4741 cgaggtcatc gcggtcgacg ccgggcgggt cgtcagccag accagcgtcc cgatcggcgc
     4801 ggtccggctg accgagcgcc acctccacca cgatccgccg atcgttccgg agatcgtcgc
     4861 gctgagcgcc gacatcgacc gtcacctcgc cgcgctggac ctgccgcgct cggtcccggt
     4921 gatcggcacc gcgggcaccg cgaccacgct ggcggcgctc aagctcggcc tcgagcgcta
     4981 cgaccccgag gcggtgaccg gcctgcggat ctcgcgcgac gcggtgaaca tcctgcacgc
     5041 gcggctgctg gcgctcagcg tcgccgagcg caagacgctg cccggcatcg agccgcagcg
     5101 cgccgacgtg atcgcggccg gcgcggcgat cttcggccgg atcctgcagc ggatcgacgc
     5161 cccggtgctg atcatctgcg atcgcggcat ccgctggggc ctcgcctacg aggcgatgtc
     5221 aaccggcggg ccggccgggg catcctgaga ccggcctggg cagcgtgcac atcgagtgac
     5281 gccggccgtc ccgatcggat tcctcggcgc gcactcgtcg ctcatcaggt cgaaccatcg
     5341 agcacgggag ccggaggcgt ccgctccgcc agcgctcgat cgcacagcgc cctgaccgcg
     5401 ccgagcgcgt acggcttgct caggaagtac ggcgttccga cgcgagccgc gacgcgctcg
     5461 agatcgagga ctccggacag caggatgatc gggatgcgct cgagaccgca gttgcgtacg
     5521 aacatctgaa gggccatccc ggggccgtcg aggttcggca tctccacgtc gagcagcacg
     5581 gcgtccggca tccggacgga cagctcgcgc agcccgacca tgccgtcgtg tgcgatccgg
     5641 acccggtgcc cgccaagcgc gagcgcctcg ccgagcagct cggccgtgtc cacgtcgtcg
     5701 tcgacgatca acagatcgcc catcacgctg gctccgcggc gccgcgcggc cccgccgccg
     5761 cgggttcggg ggacgccccg accgggagac gaaccgtgaa cgtcgtcccc gccgccggca
     5821 cgctctcgac cgcgatctcg ccgccatggg cctccacgat ccggcgcgcc acgaacaggc
     5881 cgagcccggc ccccgggatg gcgcgtgtcg ccccggcgcc gcgccggaac ggatcgaaga
     5941 cgtgctcgat ctcgtcggcg gacatgccga ccccctcatc ggccacggcg atcgcgacga
     6001 gctccggctg ctgtgccacg gtgacccgga ccgatccgcc gggcggcgag tacttgagcg
     6061 cgttgctcac caggttggtg agcacctgct cgatgcgcat cggatcgcac cgcacgcgga
     6121 ccggttcgtc gggcgcgctc atggccagcg gatgggtccc cgcggccggc ccgaacaggt
     6181 cgagcgtggc gcgcacgagc tcccgcgcgt cgcagtcctc gaccttgagc tcgaggtgac
     6241 ccgactcgat gcgagacacg tcgaggaagt cgtacgccat tcgctcgagg cgaccgatct
     6301 gtcggtcgat gcgcgcgacc agttgccgca cggccggctc gggcggtagc ggctggtcgg
     6361 gtgggatcat gccggtcgcc agcttcagcg ctcccagcgg attgcggatc tcatgggcca
     6421 ccccggccag gaacgcgagc tgatccgcgc gctggcgatc gagcgtctgc gccatgtcgt
     6481 tgaagccgcg cgcgatcgtg cggaactcct cgggctcctg ctcgctcgcg cgcgccgatc
     6541 ggtcgccgcg cgtgtagcgc tcgatcgcgc ccgagagctg gagcgccggc cggaacgtcc
     6601 tgcgttgcag ccaccaggca agtcccccga gccccaccac gagcaacagg atcgcggcca
     6661 cgccgatgac gtccgcgagc tgggaccagc gcgccgcggt gcgctccgcg gcacgcccct
     6721 gctcgacgtt gagctgcatc cactgccgcg ccagcgcaaa gaccggctcg aacgccgcgt
     6781 gcgtgtcgct ccgcacctgc gccggcgtgg ccccggtggc cgccgcgcgg tcgagcgctg
     6841 cgatgtactg gtcgacccgt cctgtcagtt cggcgaggat ctcccgttcc cgccggaggg
     6901 tgatgtactt gccggcctcc gacagctggt gtcgaatcgc gccttctctc gccgcgcgcc
     6961 cgagcgggtc ggtctcctcg cgcagcgcga tcagctccag ctcgagctcc tcgccggtgc
     7021 gcaggctctc gacctcgatg ccgacctctt ccgccgcgcg gcgcaggttc gtcgtgagga
     7081 ggatcaacgc gatcgcgacc ccggtgccga tgaccgccag gaccgcggca aagaccagaa
     7141 acgatctccg caggctcatg acagtccata cgacacggcc ccccggggca gcacggcgaa
     7201 acgaaccgcc ggcgagcgca gcaagccaag ccccgtcgag aatttctcgc gctgcgacga
     7261 tgtcgccgga ctctgacgga tcgcacgagc gagaccctcg ctggtcgcgc ggtccgccgg
     7321 tcacgatgag cgcccaggaa cgagcgccca ggagcggcgg ccaggcccag ggtcgtgagc
     7381 aggctgcgcg cgagcgttcg tcgaccgcga acgccgtgac cgtggctagt ggaactgaaa
     7441 gccgacgatc aggcgggcgt agtacgcgtc ggagtcgctg gtcaggcccg cgccgccggc
     7501 gagcgtgacc cacacgccgc gcagcacgcc ccggccgccg ccggcccacg acagcgccgg
     7561 accggcccag ctgcggttct cgacgccgcc catctcgccc ttctcggcct tgccgaacac
     7621 ctcggcgccg atcatccacg ccggcgacag cgcgtacgac gtgcccgcgg cgtactcgac
     7681 ctcggtgttc cacgtcgcgt cggtcatgcg ctcctcctcg agcgcgaggt tgccggcgat
     7741 gttcaggttg ccgatgtcct tggcgaggat cagcttgccc tcgaccctct ggtgcgcgtc
     7801 gccgcggaac agctgctgga gctcgaggta gagcaccggg tcgacgaacc actcgccgcg
     7861 gtccgccggg cggtaccggg tctcgacctt gaagccggcg tagccgctct cggtgtcgcc
     7921 cgaccggatc atgtcgagca tgttgtagag cgcgacgtcc cagcgatcgg tgatgccgta
     7981 ctcgagctcg atcatctcgc gcatgccgcg cgacg
//
LOCUS       DASVOR010000264         4072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_528405,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000264 DASVOR010000000
VERSION     DASVOR010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528405"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1018)
                     /gene="dapA"
                     /locus_tag="VF516_05630"
     CDS             complement(131..1018)
                     /gene="dapA"
                     /locus_tag="VF516_05630"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEX8107187.1"
                     /translation="MAKQATIEGCLTALVTPFRDGKVDFDGLAKLVDWQIEQGVDGIV
                     SVGTTGESATLDVEDHVAVIAATVKAARGRVPVIAGAGGNSTSEALELTRASEGAGAN
                     ALLHVTPYYNRPSQEGLFRHFEAVARVTKLPILLYNVPSRTACDLLTETVVRLAEIDN
                     IVGIKDATGSLPRGSELIARLPERMVVLSGDDSTAFPLYAVGARGVISVVSNVAPRAM
                     SDMWDAVKAGDWARGRQRHYELRVLNQLLFAEPSPAPTKAALALLGRCSVDVRLPLVQ
                     ATQGLVDQLRAEMRAQGLL"
     gene            complement(1131..1586)
                     /locus_tag="VF516_05635"
     CDS             complement(1131..1586)
                     /locus_tag="VF516_05635"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8107188.1"
                     /translation="MSGGMQALASATVGELSPRPHARVGVDDAMWKVVAEMTAKGRGA
                     VLVEDGGALVGIFTERDLLNRVDYSDALWSHVVVRDVMTPHPMVIRPDDSLSEALRRL
                     IQGHRRHLPIVDDRGHVLGLISIRDILTYIAERFPEDIMNLPPHPDHES"
     gene            complement(1583..2068)
                     /locus_tag="VF516_05640"
     CDS             complement(1583..2068)
                     /locus_tag="VF516_05640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8107189.1"
                     /translation="MRRLEKTIKEIGWTHAVPRLAVTDRVSRAFDQMSREVYDCVLVM
                     RGDELAGIFTSRDFLNRVAAVRADPEALVLGDVMTPTPRTLRPRDPVAFAINWMAVEG
                     FRNVPIVDDEGAVLGVLTVWDVMRHLEAIFDDIDATPRHVAPIEDTSAVISMIDLGGG
                     S"
     gene            complement(2191..3945)
                     /locus_tag="VF516_05645"
     CDS             complement(2191..3945)
                     /locus_tag="VF516_05645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8107190.1"
                     /translation="MKLRGRFTLALALAALVPISIAAVVTRQVIAERYRTDYAERRQA
                     AQDLVKREVDDLKKQVNQVATRLVSHDDRMIGRLLLDLAKAPRAGLDYEQRRELEQNC
                     ASIMYELALDVLTIAETGRVLCAPHARGKVGEGNHPELRDRALAGHGESFFVREDITT
                     DDGGRTGSQQVLVAEAARIAQAGAIEVAVAVGRKVDRRLLEPVRAKHRVDARIVSATG
                     DILVASDEPQWPRPSPDSPPPIRLVDASGAPVAAIEIAMSDNGMERLLRNITLNSVVV
                     SLLVLIGVVLIGLLVARRTARDLDQLVAGSLAAARGDLDHRVPVRADDEIGAVAKAFN
                     FMMEDLKTSKERLVIAERIAAWQEIARRLAHEIKNPLTPIQMAMDTLRKTWRKQHPSF
                     GEILEESTTTVMEEADRLKKIVSEFSGFARMPKPEFQRLDLNELVRSALALYQGAAPI
                     DVQLAPHLPRIDADKNQLNQVVLNLVENARDAIGQRGDGRIAVSTRLGEASDRVMLVV
                     EDNGPGIPGDLKDKVFAPYFTTKHSKGGTGLGLAIVHRIVSDHGGRIAVADTPGGGAR
                     FAIELPLRNGTALLTSRI"
BASE COUNT          578 a         1461 c         1425 g          608 t
ORIGIN      
        1 accgcggcgg tgatccgcag gtccgcccgg tcgcgcgcgg cctcgagcac cgccttgccc
       61 atccggcccg acgggccgag cacgcagacg cccggagtcc ccggagtccc cggagacgaa
      121 tgtcccgggc tcacagcagg ccctgggccc gcatctcggc gcgcagctgg tcgaccagcc
      181 cctgcgtcgc ttgcaccagc ggcaggcgga cgtcgaccga gcagcggccg agcagcgcca
      241 gggccgcctt ggtcggcgcc gggctcggct cggcgaacag cagctggttc agcacgcgga
      301 gctcgtagtg gcgctgccgg ccgcgggccc agtcgcccgc cttcaccgcg tcccacatgt
      361 cgctcatcgc gcgcggggcg acgttggaca ccaccgagat cacgccgcgc gcgccgaccg
      421 cgtagagcgg gaacgcggtg gagtcatcgc ccgacagcac gaccatgcgc tcggggagcc
      481 gcgcgatcag ctcgctgccg cgcggcaagc tgccggtggc gtccttgatg ccgacgatgt
      541 tgtcgatctc ggcgagccgc acgacggtct cggtgagcag atcgcacgcc gtccggctcg
      601 gcacgttgta caggaggatg ggcagcttgg tcacccgcgc gaccgcctcg aagtgacgga
      661 acaggccctc ctggctgggc cggttgtagt acggcgtgac gtggagcagg gcgttggccc
      721 cggccccctc cgacgcccgg gtcagctcca gcgcctcgct ggtcgagttg ccgccggcgc
      781 ccgcgatcac cgggacccgg ccgcgcgccg ccttgacggt cgcggcgatc accgcgacgt
      841 gatcctcgac gtcgagcgtc gccgattcgc cggtcgttcc gaccgacacg atgccgtcga
      901 cgccctgctc gatctgccag tcgaccagct tggccagccc gtcgaagtcg accttcccgt
      961 cgcggaaggg cgtgaccagc gcggtgagac agccttcgat cgtcgcttgc ttcgccatgg
     1021 cgtcctcgtg gccccgttcg ggcgactcca tagctacaac gtctgcgcga agaaagccat
     1081 tccggcgttg ccctgcgcgc gatgcgcagg cagccaccgg atcgccgcgg tcaggactcg
     1141 tggtcggggt gcggcggcag gttcatgatg tcctcgggga accgctccgc gatgtaggtc
     1201 aggatatcgc ggatcgagat cagcccgagc acgtggccgc ggtcgtcgac gatcggcagg
     1261 tggcggcggt ggccctggat cagccggcgc agcgcctcgc tcagcgagtc gtcgggccgg
     1321 atcaccatcg ggtgcggcgt catcacgtcg cgcaccacga cgtgggacca cagcgcgtcg
     1381 ctgtagtcca cgcggttgag cagatcgcgc tcggtgaaga tcccgaccag cgcgccgccg
     1441 tcctcgacca gcaccgcgcc gcggcccttg gccgtcatct cggcgaccac cttccacatc
     1501 gcgtcgtcga cgccgacccg cgcgtgcgga cggggcgaca gctcgccgac cgtggcgctg
     1561 gcgagcgcct gcatgccgcc gctcacgagc cacctcccag atcgatcatc gagatcaccg
     1621 ccgaggtgtc ttcgatcggg gcgacgtgcc gcggcgtcgc gtcgatgtcg tcgaagatcg
     1681 cctcgaggtg ccgcatcacg tcccacaccg tcagcacgcc gagcaccgcc ccctcatcgt
     1741 cgacgatcgg cacgttgcgg aagccctcga ccgccatcca gttgatcgcg aacgcgaccg
     1801 gatcgcgcgg ccggagcgtg cgcggcgtcg gcgtcatcac gtcgccgagc accagcgcct
     1861 ccgggtcggc gcgcaccgcc gcgacccggt tgagaaagtc gcggctggtg aagatgccgg
     1921 cgagctcgtc gccgcgcatc accagcacgc agtcgtacac ctcgcggctc atctggtcga
     1981 acgcccgcga cacccggtcg gtgaccgcca gacgcggtac cgcgtgcgtc cagccgatct
     2041 ctttgatggt cttttcgagt cgacgcatgg gacctccacg gacaggataa ccgcgtgacg
     2101 cgacgcgcgg aggggggaat ttcacggctt tgcgcgacct cccgcccccg gccccggagg
     2161 gtcaggggaa gccccctcgg gggcaatcac ctagatccgt gaggtgagga gcgccgttcc
     2221 attacggaga ggtagctcga tcgcaaaccg tgcgcctccg cccggcgtgt cggcgacggc
     2281 gatccgtccg ccgtggtccg agacgatgcg gtggacgatc gccaggccca gcccggtccc
     2341 gcccttgctg tgcttggtcg tgaagtacgg tgcgaagacc ttgtccttga ggtcccccgg
     2401 aatgcccggg ccgttgtcct cgacgaccag catcacccgg tcgctggcct cgccgagccg
     2461 ggtcgacacc gcgatccggc cgtcgccgcg ctgaccgatc gcgtcacgcg cgttctcgac
     2521 caggttgagc acgacctggt tgagctggtt cttgtcggcg tcgatccggg gcaggtgggg
     2581 cgcgagctgg acatcgatcg gcgccgcgcc ctggtacagc gccagcgccg agcgcaccag
     2641 ctcgttgagg tcgagccgct ggaactcggg cttgggcatc cgggcgaacc ccgagaactc
     2701 ggagacgatc ttcttgagcc ggtcggcctc ctccatcacc gtcgtggtcg actcctcgag
     2761 gatctcgccg aacgacgggt gctgcttcct ccaggtcttg cgcagcgtgt ccatcgccat
     2821 ctggatcggg gtcagcggat tcttgatctc gtgggcgagc cggcgcgcga tctcctgcca
     2881 cgccgcgatc cgctccgcga tcaccaggcg ctccttggag gtcttgaggt cctccatcat
     2941 gaagttgaag gccttggcga ccgcgccgat ctcgtcgtcc gcgcgcaccg gcacgcggtg
     3001 gtcgaggtcg ccgcgcgcgg cggcgagcga gccggcgacc agctgatcga gatcgcgggc
     3061 ggtgcggcgc gcgaccagca ggccgatcag cacgacgccg atcagcacga gcagcgacac
     3121 caccaccgag ttcagcgtga tgttgcgcag caggcgctcc atgccgttgt ccgacatcgc
     3181 gatctcgatc gcggcgaccg gcgcgccgct cgcatcgacc agccggatcg ggggcggcga
     3241 gtccggcgat ggccgcggcc actgcggctc gtcggaggca accaggatgt cgccggtggc
     3301 ggacacgatc cgcgcgtcga cccggtgctt ggcgcgcacc ggctcgagca gccgccggtc
     3361 gaccttgcgg ccgaccgcga ccgcgacctc gatcgcgccg gcctgcgcga tccgggcggc
     3421 ctcggcgacc agcacctgct gcgacccggt cctgccgccg tcgtcggtcg tgatgtcctc
     3481 gcggacgaag aacgactcgc cgtggccggc gagcgcgcga tcgcggagct cggggtgatt
     3541 gccctcgccg accttgccgc gcgcgtgggg cgcgcacagc acgcggccgg tctcggcgat
     3601 cgtcagcacg tcgagcgcga gctcgtacat gatcgaggcg cagttctgct cgagctcgcg
     3661 gcgctgctcg tagtcgaggc cggcccgggg cgccttggcg agatcgagca gcaggcggcc
     3721 gatcatgcga tcgtcgtggc tgaccagccg ggtcgcgacc tggttgacct gcttcttgag
     3781 gtcgtcgacc tcgcgcttga ccagatcctg cgcggcctgc ctgcgctcgg cgtagtcggt
     3841 gcggtagcgc tcggcgatca cctggcgggt gaccaccgcg gcgatgctga tcgggaccag
     3901 cgcggcgagc gcgagcgcga gcgtgaaccg gccgcggagc ttcatggcgg cgccttgaac
     3961 cagtacaggt cggcgagcga cggccggccg ctcgcgtcga acccgaggcc gtgcacctcg
     4021 ttgtggtgcc agatcaagag cgagcggaac atcagcggca cgatcggcag cg
//
LOCUS       DASVOR010000265         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265298,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000265 DASVOR010000000
VERSION     DASVOR010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265298"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..1018
                     /locus_tag="VF516_05650"
     CDS             50..1018
                     /locus_tag="VF516_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX8107191.1"
                     /translation="MGNVLAFCEFNGAQLRSSALANLSFARQAAQAHGGEVIALLVGT
                     GAAAAGPAAAKYAPKVVVVDAPPLANYLAETFAPIVAEVARAQGATVISATATAVGKD
                     LAPRVAGLLDAGMASDIAKLAGKDTFVRPILAGNAYSTVQVATPIIVVTPRQSEFEAA
                     AELGSPGTVTTAAPGNVDALGAEFVRLDAQKSDRPDLSDAKVVVSGGRGMKNGENFKV
                     LEQLADLLGAAMGASRAATDAGMVPADWQVGQTGKIVAPNLYFAVAISGAIQHLAGMK
                     GSKTIVAINKNKDEPIFQVADYGVVAEWEKAVPELIAEIKKLKASR"
     gene            1128..>1659
                     /locus_tag="VF516_05655"
     CDS             1128..>1659
                     /locus_tag="VF516_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEX8107192.1"
                     /translation="MAFAQTADRRPIAGAVATLGVSDRVEFLRKTYGLLGVALVGFAV
                     LTAGMMRFATETSLRFSAWALTGRLNWLLVIGLFMVVGYIARRLAVSETSRGLQLLGL
                     GLYIFVQSALLQPILWILIFRFGDRAMLGSGVLLSGQAGSILMQAVVITLAIFIGLTL
                     TVFITKKDFSFLGGILT"
BASE COUNT          249 a          569 c          581 g          260 t
ORIGIN      
        1 ccctcgtgaa gaagctcgcc gatgaggcga aggtcctgta ggagccgcca tgggtaacgt
       61 cctcgcgttc tgtgaattca acggcgcgca gctgcgctcg agcgcgctgg ccaacctgtc
      121 gttcgcccgg caagccgcac aggcccacgg cggcgaggtg atcgcgctgc tggtcggcac
      181 cggcgcggcc gcggccgggc ccgccgcggc gaagtacgcc cccaaggtgg tcgtcgtcga
      241 cgcgcccccg ctggcgaact acctcgccga gacgttcgcg ccgatcgtcg ccgaggtggc
      301 gcgcgcgcag ggcgccacgg tgatctcggc gaccgcgacc gcggtcggca aggatctcgc
      361 gccgcgggtc gccgggctgc tcgacgccgg gatggcgtcg gacatcgcca agctcgccgg
      421 caaggacacg ttcgtccggc cgatcctggc cggcaacgcg tactccaccg tgcaggtcgc
      481 gacaccgatc atcgtcgtga cgccgcgcca gtccgagttc gaggccgcgg ccgagctggg
      541 gtcgcccggc acggtgacca ccgcggcgcc cggcaacgtc gacgcgctcg gcgccgagtt
      601 cgtccggctc gatgcccaga agagcgaccg gccggacctg tcggacgcca aggtcgtggt
      661 gtccggcggc cgcggcatga agaacggcga gaacttcaag gtcctggagc agctcgccga
      721 cctgctcggc gcggcgatgg gcgcgtcgcg cgccgcgacc gacgccggca tggtcccggc
      781 ggactggcag gtcgggcaga ccggcaagat cgtcgcgccg aacctgtact tcgcggtcgc
      841 gatctcgggg gcgatccagc acctggccgg gatgaagggc tccaagacga tcgtcgcgat
      901 caacaagaac aaggacgagc cgatcttcca ggtcgccgac tacggcgtgg tcgcggaatg
      961 ggagaaggcg gtcccggagt tgatcgcgga gatcaagaag ctcaaggcca gccgctagcc
     1021 cgccggcccg ccgatcgggc agtcccggcg ggcgtacgag taggtgcgac acccgcggct
     1081 tgcctcgaca cgaaccgtgc tacaagtagc tgaaaggcag gctctcgatg gcattcgcac
     1141 aaaccgctga tcgtcgtccc atcgcaggcg ccgtcgcgac cctcggtgtc agcgaccgcg
     1201 tcgagttctt gcgcaagacc tacggactgc tcggcgtcgc cctggtcggg ttcgccgtgc
     1261 tcaccgccgg catgatgcgg ttcgcgaccg agaccagtct ccggttctcg gcatgggcgc
     1321 tcacgggccg cctgaactgg ctgctcgtca tcggcctgtt catggtggtg ggatacatcg
     1381 cccgccgcct cgccgtctcg gagacctcgc ggggtctgca gctgctcggg ctcggcctgt
     1441 acatcttcgt gcaatcggcg ctgctccagc cgatcctgtg gatcctgatc ttccggttcg
     1501 gcgaccgtgc gatgctgggc tcgggcgtgc tgctcagcgg ccaggccggg tcgatcctga
     1561 tgcaggcggt ggtgatcacg ctggcgatct tcatcggcct gaccctcacg gtgttcatca
     1621 ccaagaagga cttctcgttc ctgggcggga tcttgacga
//
LOCUS       DASVOR010000266         2232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382308,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000266 DASVOR010000000
VERSION     DASVOR010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2232
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382308"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2194)
                     /locus_tag="VF516_05660"
     CDS             complement(<1..2194)
                     /locus_tag="VF516_05660"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX8107193.1"
                     /translation="MTVSPATATVVVAGTQTFTAAAFDAAQQPISGVTFTWTSSDPTI
                     ATVNAGGVATGVAAGQATITAAAPNGVSGSAALQVTPAPQPGIPDTRFSELHYDNTGV
                     DTGEAIEIEGPAGMNVAGWTIVLYDGTNRTAYSTQTLSSAIPATCGARGVVVVNYPVN
                     GIQNGSPDGMALVDAAGHVVEFLSYEGTFTATGGPANGVVSTDIIAQEPSTTPIGQSL
                     QRNRDGTWTGPVDNSFGLCNPDRTIQHNNLTFSGRAPSDPALPVGFQSQLFATERTAG
                     NATVNTTFTWSSDTPDIATVDQFGVITALAAGTAVFRATAADGTTGTWPLPTTVGVAS
                     TTASYVGNTEFGEPADSDPSDDFIVRHAEYTTDYNSNRGEPNWVSYDLNATDFGPQDR
                     CNCFTFDPDLPASFTHLTTQDYTGAGAAAGFGIDRGHMTRSFDRTTSNLDNAHTFLLS
                     NVVPQASDLNQGPWAIFEDFLGDLAMNQNKEVRIITGPAGSQGTVKNEGKIVIPASTW
                     KVAVIQDRGHGLADIRDYRDVEVIAVNMPNVAGIRNVDWHTYLTTVDAIEALTGYDLL
                     ARLPDKVENIVEAGIKPPIAAIDGPYAGAEGGGIAMSAAASVDPNGTVVSYAWDFGDG
                     TTGIGPSLAHTYARGGVYTVSVTVTDNDGLTDNIQAIATVAIVPPIAALDGPYAGAEG
                     GAVAMSAAASRDPNGMVVSYAWDFGDGTKGTGPTALHTYAQDGVYTVTV"
BASE COUNT          277 a          733 c          836 g          386 t
ORIGIN      
        1 tgacggtgac cgtgtagacg ccgtcctgcg cgtaggtgtg cagcgcggtc gggccggtgc
       61 ccttcgtgcc gtcgccgaag tcccacgcgt agctcaccac catgccgttg ggatcgcgcg
      121 acgctgccgc gctcatcgcg accgcgccgc cctcggcccc ggcgtacggc ccgtcgagcg
      181 ctgcgatcgg cggcacgatg gccacggtgg cgatcgcctg gatgttgtcg gtgagcccgt
      241 cgttgtcggt gaccgtgacg ctgaccgtgt agacgccgcc cctcgcgtag gtgtgcgcaa
      301 ggctcggacc gatccccgtc gtgccgtcgc cgaagtccca cgcgtagctg accaccgtgc
      361 cgttgggatc gaccgacgcc gccgcgctca tcgcgatgcc gccgccctcg gcgccggcgt
      421 acgggccgtc gatcgccgcg atcggcggct tgatgccggc ctcgacgatg ttctcgacct
      481 tgtcgggcag cctggcgagc aggtcgtagc cggtcagcgc ctcgatcgcg tcgaccgtgg
      541 tgaggtaggt gtgccagtcc acgttccgga tcccggccac gttgggcatg ttgaccgcga
      601 tgacctcgac gtcgcggtag tcgcggatgt cggcgagccc gtggccgcga tcctggatca
      661 ccgcgacctt ccacgtgctg gccgggatca cgatcttgcc ctcgttcttg accgtgccct
      721 ggctgcccgc ggggccggtg atgatgcgga cctccttgtt ctggttcatc gccagatcgc
      781 cgaggaagtc ctcgaagatc gcccacgggc cctggttgag gtcgctggcc tgcggcacga
      841 cgttggacag caggaacgtg tgcgcgttgt cgaggttcga cgtcgtgcga tcgaacgacc
      901 gggtcatgtg gccgcggtcg atgccgaacc cggcggccgc gccggcgccg gtgtagtcct
      961 gggtcgtgag gtgggtgaac gacgccggga gatcggggtc gaacgtgaag cagttgcagc
     1021 ggtcctgggg gccgaaatcc gttgcgttga ggtcgtagct cacccagttg ggctcgccgc
     1081 gattggagtt gtagtccgtg gtgtactcgg cgtggcggac gatgaaatca tcgctcggat
     1141 cggagtcggc cggctcgccg aactcggtgt tgccgacgta cgacgcggtc gtgctcgcaa
     1201 ccccgacggt ggtcggcagc ggccaggtcc cggtggtgcc gtccgcggcg gtcgcacgga
     1261 acaccgcggt gcccgcggcg agcgcggtga tcacgccgaa ctggtcgacg gtcgcgatgt
     1321 ccggcgtgtc ggacgaccag gtgaacgtcg tgttcaccgt ggcgttgccg gcggtgcgct
     1381 cggtggcgaa cagctgggac tggaacccga cgggcagcgc cggatcgctc ggggcgcggc
     1441 cgctgaaggt gagattgttg tgctggatcg tccgatcggg gttgcacagg ccgaacgaat
     1501 tgtcgaccgg gccggtccag gtcccgtcgc ggttgcgctg cagcgactgg ccgatcggcg
     1561 tcgtcgaggg ctcctgggcg atgatgtcgg tcgacaccac cccgttcgcc ggcccgccgg
     1621 tcgcggtgaa cgtgccctcg taggacagga actccacgac gtgaccggcg gcgtcgacca
     1681 gcgccatgcc gtcggggcta ccattctgga tcccgttgac cgggtagttg acgacgacca
     1741 cgccgcgggc gccgcaggtc gcggggatcg cgctgctcag cgtctgggtg ctgtaggccg
     1801 tgcggttggt gccgtcgtag agcacgatgg tccagccggc gacgttcatg ccggccgggc
     1861 cctcgatctc gatcgcctcg ccggtgtcga cgccggtgtt gtcgtagtgc agctcgctga
     1921 accgggtgtc ggggatcccg ggctggggcg cgggggtgac ctgcagcgcc gccgagcccg
     1981 acacgccgtt gggtgccgcg gccgtgatcg tcgcctggcc ggccgccacg ccggtcgcca
     2041 cgccgccggc gttgaccgtc gcgatcgtcg gatcgctgga cgtccaggtg aacgtcacgc
     2101 cggagatcgg ctgctgggcg gcgtcgaacg cggccgccgt gaaggtctgg gtgccggcga
     2161 ccacgaccgt cgcggtcgct ggcgacaccg tcacgctcgt gaccaccggc gggggcggcg
     2221 tcgcgtcgtt gt
//
LOCUS       DASVOR010000267         4743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_410905,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000267 DASVOR010000000
VERSION     DASVOR010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4743
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_410905"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..800)
                     /locus_tag="VF516_05665"
     CDS             complement(78..800)
                     /locus_tag="VF516_05665"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX8107194.1"
                     /translation="MFLEDMTWSEAERALTRDTVVVVPIGAASKEHGPHLRLANDWIM
                     ADYLRRRLAERTAVVVAPTVGLSFYPAFVEYPGSISLELDTARDVIVQVVRSLARHGP
                     RRFYALNTGISTLRALRPAAELLAADGIVLRYTDLSKALAPVEKQVAQQEGGTHADEL
                     ETSMMLYIAPDTVDMARAVKDYHPGTGPLTRKSDGPGTYSPTGSWGDPTLATRAKGER
                     LGRALLDALVAEVEALRAAPLP"
     gene            complement(964..2907)
                     /locus_tag="VF516_05670"
     CDS             complement(964..2907)
                     /locus_tag="VF516_05670"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEX8107195.1"
                     /translation="MAQTKYTGEEIQVLEGLDPVRKRPGMYIGGTGKDGYHHLLWEVV
                     DNSIDEVINKHASRVEVTLHKDGKSATVEDNGRGIPVDVMPKFKKSAVEVIFTTLHSG
                     GKFERGKSYAVSGGLHGVGAAVVNALSSELVVTVKRDGERHEITFERGDATSKLKRLG
                     AARGTGTTVRFRPDEDVFGAKLGFDAELIAERLEAKSYLHGGLEIVFTDETKSPVQTR
                     SFVHPQGIAEYLPKLVAERGKPPVPPSGAVFYVEKRDDTARLAIELALQWTEGTDDLI
                     KTYVNSVPTPDGGTHDAGFRAAVVKAIRNYIATHKLDPKGVTLTAEDIREGVVAVLST
                     YVLDPQFQSQTKNRLNNPEVAGQVEGLVRPALENYLNSNPNWAQAVVARVIIAARARE
                     ASRAAHQAVTRKTAVSHRLNLPGKLADCSSTDPGDSELFIVEGESAGGSAKQGRDRRT
                     QAILPLRGKVLNAEQANNQKLLANKELQDIVSALGCGMGESIDLGKLRYGKIFLLMDA
                     DADGHHIATLLLTFFFRYMKPLIDQGHLYLAQPPLYRIDIGKQTLWALDDEHKEQLIK
                     ANARGNAKPNITRFKGLGEMNPDTLKMTTLDPKTRQPLRVTVKDGEQLRTDQMISDLM
                     GKDVAARFKMITENAADIGELDV"
     gene            complement(2959..>4743)
                     /locus_tag="VF516_05675"
     CDS             complement(2959..>4743)
                     /locus_tag="VF516_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8107196.1"
                     /translation="PHNLGEITNALIALADNRDLDHVQLMKHIKGPDFPTGGQLLNSK
                     TELREIYKLGQGTIRVRGEYKREERKRGGTDIVITSIPYALTKRDVVEKIAEVIVSRK
                     LPYLLDVRDESTTDVRIVLEIKKDADPELVMAYLFKHTPLQSNFHVNMTCLVPPGFLD
                     DESGRKLPADAPPQPRKMGIKEMLGHFLDFRLEVVERRFKYQLAQLEARIHILEGFVA
                     IFDALDQLIKLIRTSDGKEDAAAKIIKQFKLDEAQTDAILELKLYKLAKLEIHVIREE
                     LAQKTGEAKKIRAILKSEDKLWTVIKDELRATAAELDTKRRTDPTGAGEEVTFDADAF
                     IVDEDAHIVVTRDGWIKRVRELKDPNQTRVREGDEVTHVLAGSTKEKVIFFTNRGSGY
                     VIKINDIAATTGYGDPAQKYFKFADGERIVSAMTLDPRAMVAPTLLALSRNGFGLRFT
                     TSAHAEITTKAGRRYAKPGEGDEIIGVVACNDSDVVVAATRDGHVLLCKADEIAKLEG
                     PGRGVTVIKTEDDDLVIGFIAGAKGDQLVLETEKGGKRFEIAADPKQVRARGGRGHQI
                     IKRAQLVVVPPPVKVQPLANAEGGQGVN"
BASE COUNT          614 a         1601 c         1657 g          871 t
ORIGIN      
        1 gctgcgagtc tggcacatcc cgcgccggcc acgccgggac gagagccagg tggctaccct
       61 ggtctgggtc gcgatcgcta cggcagcggc gccgcgcgca gcgcctcgac ctcggcgacc
      121 agcgcatcga gcagcgcgcg ccccagccgc tcgcccttgg cacgggtcgc cagcgtcgga
      181 tcgccccagc tgccggtcgg cgagtaggtc cccgggccgt ccgacttgcg ggtcaagggg
      241 ccggtcccgg ggtggtagtc cttgaccgcg cgcgccatgt cgacggtgtc gggagcgatg
      301 tacagcatca tcgaggtctc gagctcgtcg gcgtgcgtgc cgccctcctg ctgcgcgacc
      361 tgcttctcca ccggcgccag cgccttcgac aggtcggtgt agcgcagcac gatgccgtcg
      421 gccgccagca gctcggccgc gggccggagc gcgcgcagcg tcgagatccc ggtgttgagc
      481 gcgtagaacc ggcgcgggcc gtggcgcgcc aggctgcgca cgacctgcac gatcacgtcg
      541 cgcgcggtgt cgagctccag gctgatcgac cccggatact cgacgaacgc cgggtagaac
      601 gacagcccga cggtcggcgc gaccaccacg gccgtgcgct ccgcgagccg gcgccggagg
      661 tagtcggcca tgatccagtc gttggccagc cgcaggtgcg gcccgtgctc cttggatgcc
      721 gcgccgatcg gcacgacgac gacggtgtcg cgggtcagcg cccgctcggc ctcggaccag
      781 gtcatgtctt cgaggaacat ggtgggtatc ctgcgagccg ccgcgggcgg ccgccccgga
      841 gcctgccccg cacatgcggc caggacaagg accaggacgg cggcggagcc ggggcgtgat
      901 gacatcgacc gagcgtatcg cacgcagcca ccgcgctccc gcgcacgtga agccggccga
      961 gccttacacg tcgagctcgc cgatgtcggc cgcgttctcg gtgatcatct tgaaccgcgc
     1021 cgcgacgtcc ttgcccatca ggtcggagat catctggtcg gtgcgcagct gctcgccgtc
     1081 cttgacggtg acgcgcagcg gctggcgcgt cttcgggtcg agcgtggtca tcttgagcgt
     1141 gtcggggttc atctcgccga ggcccttgaa ccgggtgatg ttcggcttgg cgttgcccct
     1201 ggcgttggcc ttgatcagct gctccttgtg ctcgtcgtcg agcgcccaca gcgtctgctt
     1261 gccgatgtcg atccggtaca gcggcggctg ggcgaggtag aggtggccct gatcgatcag
     1321 cggcttcatg tagcggaaga agaacgtgag caagagcgtc gcgatgtggt ggccatcggc
     1381 gtcggcgtcc atcagcagga agatcttgcc gtagcgcagc ttgccgaggt cgatcgactc
     1441 gcccatgccg cagccgagcg ccgagacgat gtcctggagc tccttgttgg cgagcagctt
     1501 ctggttgttg gcctgctcgg cgttgagcac cttgccgcgc agcgggagga tcgcctgggt
     1561 ccggcggtcg cggccctgct tggccgagcc gccggccgac tcgccctcga cgatgaacag
     1621 ctcgctgtcg cccggatcgg tcgagctgca gtcggcgagc ttgccgggca ggttgagccg
     1681 gtgcgacacc gcggtcttgc gggtgaccgc ctggtgcgcc gcgcgcgacg cctcgcgcgc
     1741 ccgggccgcg atgatcacgc gcgcgaccac cgcctgggcc cagttggggt tgctgttgag
     1801 gtagttctcc agcgcggggc ggaccaggcc ctcgacctgg ccggcgacct cggggttgtt
     1861 gagccggttc ttggtctggc tctggaactg cggatcgagg acgtaggtcg acagcaccgc
     1921 gaccacgccc tcgcggatgt cctcggcggt cagcgtcacg cccttgggat cgagcttgtg
     1981 ggtcgcgatg tagttgcgga tcgccttgac caccgcggcg cggaacccgg cgtcgtgggt
     2041 gccgccgtcg ggggtcggca ccgagttgac gtaggtcttg atcaggtcgt cggtcccctc
     2101 ggtccactgc agcgcgagct cgatcgccag ccgcgccgtg tcgtcgcgct tctcgacgta
     2161 gaacaccgcg ccgctgggcg gcaccggcgg cttgccgcgc tcggcgacca gcttgggcag
     2221 gtactcggcg atgccctggg ggtggacgaa gctccgggtc tggaccggcg acttggtctc
     2281 gtcggtgaac acgatctcga ggccgccgtg caggtagctc ttggcctcga gccgctcggc
     2341 gatcagctcg gcgtcgaacc cgagctttgc gccgaacacg tcctcgtcgg ggcggaaccg
     2401 cacggtggtg ccggtgccgc gcgccgcgcc cagccgcttg agcttgctgg tcgcgtcgcc
     2461 gcgctcgaac gtgatctcgt ggcgctcgcc gtcgcgcttg accgtgacga ccagctcgct
     2521 cgacagcgcg ttgaccaccg cggcgccgac gccgtgcagg ccgccggaca ccgcgtagct
     2581 cttgccgcgc tcgaacttgc cgccggagtg cagcgtggtg aagatgacct cgaccgccga
     2641 cttcttgaac ttgggcatca cgtcgaccgg gatgccgcgg ccgttgtcct cgacggtggc
     2701 gctcttgccg tccttgtgca gggtgacctc gacccgcgag gcgtgcttgt tgatcacctc
     2761 gtcgatcgag ttgtcgacca cctcccacag caggtggtgg tagccgtcct tgccggtgcc
     2821 gccgatgtac atgcccggcc gcttgcggac cgggtcgagg ccctcgagga cctggatctc
     2881 ttcaccggtg tacttggtct gcgccatctt catacctcgc tggcatgcct cgctggcatg
     2941 cctcgctgga cgtacggcct agttgactcc ctgtccaccc tcggcgttgg cgagcggctg
     3001 gaccttgacc ggcggcggca ccacgacgag ctgggcccgc ttgatgatct ggtggcccct
     3061 gcccccgcgg gcccggacct gcttgggatc ggcggcgatc tcgaaccgct tgccgccctt
     3121 ctcggtctcg agcacgagct ggtcgccctt ggcgccggcg atgaagccga tcaccaggtc
     3181 gtcgtcctcg gtcttgatca cggtgacgcc gcggcccggg ccctcgagct tggcgatctc
     3241 gtcggccttg cacagcagca cgtgaccgtc gcgggtcgcg gcgaccacga cgtcgctgtc
     3301 gttgcacgcg accacgccga tgatctcgtc gccctcgccc ggcttggcgt agcggcggcc
     3361 ggccttggtg gtgatctcgg cgtgcgccga ggtggtgaac cgcaggccga agccgttgcg
     3421 cgagagcgcg agcagggtcg gcgcgaccat cgcgcgcgga tcgagcgtca tcgccgacac
     3481 gatgcgctcg ccgtcggcaa acttgaagta tttctgcgcc gggtcgccgt acccggtggt
     3541 cgcggcgatg tcgttgatct tgatgacgta gcccgagccc cggttggtga agaagatcac
     3601 cttctccttg gtcgagccgg ccagcacgtg ggtgacctcg tcgccctcgc ggacccgggt
     3661 ctggttcgga tccttgagct cgcgcacccg cttgatccag ccgtcgcggg tcaccacgat
     3721 gtgggcgtcc tcgtcgacga tgaacgcgtc ggcgtcgaac gtgacctcct cgcccgcgcc
     3781 cgtggggtcc gtcctccgct tggtatcgag ctcggcggcg gtggcccgga gctcgtcctt
     3841 gatcaccgtc cacagcttgt cctcgctctt caggatcgcc cggatcttct tggcctcgcc
     3901 ggtcttctgg gcgagctcct cgcggatcac gtggatctcc agcttggcga gcttgtagag
     3961 cttgagctcg aggatcgcgt cggtctgcgc ctcgtcgagc ttgaactgct tgatgatctt
     4021 ggccgcggcg tcctccttgc cgtcggacgt gcggatcagc ttgatcagct ggtcgagcgc
     4081 gtcgaagatc gcaacgaatc cttctagtat gtgaatccgg gcctcgagct gggcgagctg
     4141 gtacttgaac cggcgctcga cgacctcgag ccggaagtcg aggaagtggc ccagcatctc
     4201 cttgatcccc atcttgcgcg gctggggcgg cgcatcggcg ggcagcttcc ggccggactc
     4261 gtcgtcgagg aacccgggtg gcaccaggca cgtcatgttg acgtggaagt tggactgcag
     4321 cggcgtgtgc ttgaacaggt acgccatcac cagctcgggg tcggcgtcct tcttgatctc
     4381 gagcacgatg cgcacgtcgg tcgtcgactc gtcgcgcaca tcgagcaagt acggcagctt
     4441 gcgcgacacg atgacctcgg cgatcttctc gaccacgtcg cgcttggtca gcgcgtaggg
     4501 gatcgaggtg atcacgatgt cggtgccgcc gcgcttcctc tcctcgcgct tgtactcgcc
     4561 gcgcacccgg atcgtgccct gaccgagctt gtagatctcg cgcagctcgg tcttgctgtt
     4621 gagcagctgg ccgccggtcg ggaagtccgg ccccttgatg tgcttcatca gctggacgtg
     4681 gtcgaggtcg cggttgtcgg ccagggcgat caacgcgttg gtgatctcgc cgaggttgtg
     4741 agg
//
LOCUS       DASVOR010000268         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_440750,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000268 DASVOR010000000
VERSION     DASVOR010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440750"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1071)
                     /locus_tag="VF516_05680"
     CDS             complement(148..1071)
                     /locus_tag="VF516_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107197.1"
                     /translation="MPRLGLGDLIGAGPRAVSRYTGTLLAVFVAQSLIAASCIVAIAV
                     VLAHTFSHLPMFDEAVDGDFVALIYSVRYGKDSMVATAGIVFGAVMLWQLASWFVIGG
                     ACSVLAQRPEGRGDTARCFGAGGASTYLAYARLALCAMPGWLVVLFLLAFGAGLVKGR
                     IDHALTLGELFGATAPVVLLAALLLHILWTVEDYARVELTLRQDTHDPGVVATYLRSL
                     SYVLRRPVTLVHAALGWLVFALVSLAYFYLSLGHPMYGAEGAITLFIIRQGVSLLRMA
                     IRFGVLAGQVELGRTRPLPPRRVEVKVDPKP"
     gene            complement(1074..>1960)
                     /locus_tag="VF516_05685"
     CDS             complement(1074..>1960)
                     /locus_tag="VF516_05685"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX8107198.1"
                     /translation="GHNWFQGMLASNEAEEAWLDEGVNEWADAHAMAEIYGPRGSLID
                     WMGWQAEISAVTRAIDVSRQPVPSPIATAAYAFVDSGAYGDASYPMTARALATLEHTV
                     GSQRFMAAMKTYAKTFAFRHPTGRDLFATLAHELDQDLGWYFGPVFHEVGQLELELRS
                     AKCRPAHEPRGVFGDGSARKTRTELEAPATGNWTCEVVVQNTGVIHVPVDIELKFADG
                     STKQLRWDDRGNGNWERFVVERSSRLTEVWLDPEAKIALDNPTKHHYRIDGDGSAALR
                     ASAWFGWFTQTVLQIVGP"
BASE COUNT          301 a          675 c          729 g          255 t
ORIGIN      
        1 ggcgatggtg gcgcggccgg cgagggccgc cgccggagca gccgtccgtg ccgcgcgact
       61 tgccccagtc gacgctggtc gaccgctgat ccgccgaggc gagctcgcga agcgcaacct
      121 gtaagtcgta cttacaagtt cgccggctca cggcttcgga tcgaccttga cctcgacgcg
      181 gcggggcggc agcggccggg tccggccgag ctcgacctgg ccggcgagca cgccgaaccg
      241 gatcgccatg cggagcagcg acacgccctg gcggatgatg aacagcgtga tcgcgccctc
      301 ggcgccgtac atcgggtggc cgagcgagag gtagaagtac gcgaggctga ccagggcgaa
      361 caccagccac ccgagcgcgg cgtggaccag cgtgaccggc cggcgcagga cgtagctcag
      421 cgagcgcagg taggtcgcga cgacgccggg atcgtgggtg tcctggcgca gggtcagctc
      481 gacgcgcgcg tagtcctcga cggtccacag gatgtggagc agcagcgcgg cgagcaggac
      541 cacgggcgcg gtcgcgccga acagctcgcc cagggtcagc gcgtgatcga tccggccctt
      601 gaccaggccg gcgccgaacg cgagcaggaa cagcacgacg agccagccgg gcatcgcgca
      661 cagcgcgagc cgggcgtagg cgaggtaggt cgacgcgccg ccggcgccga agcagcgcgc
      721 ggtgtcgccg cggccctcgg ggcgctgggc gagcacgctg caggcgccgc cgatcacgaa
      781 ccacgacgcg agctgccaca gcatgaccgc gccgaacacg atgccggcgg tcgcgaccat
      841 gctgtccttg ccgtagcgca cggagtagat gagcgcgacg aagtcgccgt cgaccgcctc
      901 gtcgaacatc ggcaggtgcg agaacgtatg ggcgaggacg acggcgatcg cgacgatgca
      961 gctcgcggcg atcaggctct gggcgacgaa caccgcgagc agcgtgccgg tgtagcgcga
     1021 caccgcgcgc gggccggcgc cgatcaggtc tccgagtccg agccgcggca tggtcagggc
     1081 cccacgatct gcaggacggt ctgggtgaac cagccgaacc acgccgaggc gcgcagcgcg
     1141 gccgagccgt cgccgtcgat ccggtagtgg tgcttggtcg ggttgtcgag cgcgatcttg
     1201 gcctcggggt cgagccagac ctcggtcagc ctggagctgc gctcgaccac gaagcgctcc
     1261 cagttgccgt tgccgcggtc gtcccagcgc agctgcttgg tcgagccgtc ggcgaacttg
     1321 agctcgatgt cgaccggcac gtggatcacg ccggtgttct ggaccacgac ctcgcaggtc
     1381 cagttgcccg tcgccggggc ctcgagctcg gtccgggtct tgcgagccga gccgtcgccg
     1441 aacacgccgc gcggctcgtg ggcgggccgg cacttcgcgc tgcgcagctc gagctcgagc
     1501 tggccgacct cgtggaacac cgggccgaag taccagccga ggtcctgatc gagctcgtgg
     1561 gcgagcgtgg cgaacaggtc gcggccggtc gggtggcgaa acgcgaacgt cttggcgtag
     1621 gtcttcatcg cggccatgaa ccgctggctg ccgacggtgt gctccagcgt ggccagcgcc
     1681 cgcgcggtca tcgggtagct cgcgtcgccg tacgcgccgg agtcgacgaa cgcgtaggcc
     1741 gcggtcgcga tcggcgacgg gaccggctgc cggctgacgt cgatcgcgcg cgtcaccgcc
     1801 gagatctcgg cctgccagcc catccagtcg atcagcgagc cgcgcgggcc gtagatctcg
     1861 gccatcgcgt gggcgtcggc ccactcgttg acgccctcgt cgagccaggc ctcctcggcc
     1921 tcgttcgacg ccagcatgcc ctggaaccag ttgtggccga
//
LOCUS       DASVOR010000269         5973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353218,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000269 DASVOR010000000
VERSION     DASVOR010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353218"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF516_05690"
     CDS             complement(<1..219)
                     /locus_tag="VF516_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX8107199.1"
                     /translation="MAPERASHQGDDLDVIIVGAGLSGVGAGYRLQTECPTKRFAILE
                     GRDAIGGTWDLFRYPGIRSDSDMFTLGYP"
     gene            489..2348
                     /locus_tag="VF516_05695"
     CDS             489..2348
                     /locus_tag="VF516_05695"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8107200.1"
                     /translation="MAPLGSRIPILGPVLGLLLAVPACSSSPAHPPASAAAPASGEPS
                     SPPAGSPQGATPPAAQPVDPAASPDQAFAAFAQHFLDEMLRRSPTFATHAGDHRHDDR
                     WPDVSVQGQATDRSFLEETRAALARLPRERLSEQNQIDAAILDERLRATLFSLEELKP
                     YDTDPMAYVGLISNGIDPLVTREFGTRESRMASLAGRLDGIPAIVAVARQRLAHAAKV
                     FTETAIQQNKGLIALVETQLPARFAEVPAQKDRLTAAAARAATALHDLQRFLETDLLP
                     RSDGSFRLGRERFARKLAFALGEDVDIDTIAADARALLTRIQAEMVDTARQIWADDKL
                     GKLPPLDTAEQKKAFVKRVLDHVAADRPTNQTILADARRWLDKATAFVRDQNLVRVPD
                     EPVAVIEMPEYKRGVAVAYCDSSGALEATPQTFFAISPTPSDWPRARAESFYREYNQA
                     MLADLTIHEAMPGHYLQLMHNNQFASKLRAVFASGPFVEGWAVYAEWMMAEHGFGGPR
                     VKLQRQKMMLRTCANAILDHDIHAGTMDEQAALALMKNEAFQEDGEAVGKWKRARLSS
                     AQLTTYFYGFSELLKLRRAVESQPGFSERAYHDRLLSWGSPPMRYVRQLVARH"
     gene            3391..>5973
                     /locus_tag="VF516_05700"
     CDS             3391..>5973
                     /locus_tag="VF516_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107201.1"
                     /translation="MSNIQVVQVLYGTGSYIPQVTSTSTPSMATFYQGVLNSPYVDWL
                     TEYNTVGLPPPSSNQVLGRGSFSQQVQITPNPANNGTLIDDLQIQNELSAQIAAGTLP
                     APTHDPAGNNNTYYALFFPHGKVISLGGSLSCQVFCAYHGTIASAGGQGEIEYGVHPD
                     FQPGSGCENGCGAAATQFGNYTQVASHELIETMTDPEVGLATVFGPPLAWADTVFSEI
                     GDICNDQNGQVAGADGVTYDVQTEFSNSVNDCIVINPAATPVTATSAGEACRGTTALA
                     TVTVLGGAGRFTSPVTLSVTGVSPTPPPGGEITATFDPDPVPTPPVNGATSTMRISST
                     PLTPPGTYTLTIQAASTGLTRTTTTQVVIRSEVPNGPTLTSPVNGADGVAQAATFTWS
                     PVDQATQYELDVFAGSDCTGTPVRTFTTGNTNFTIPQAQALDTFQNFSWHVSAANSCG
                     SSATSACFNFRTASCSDPIEAVTNGGFENGLPGWTTELAVPPPVVSSAHVHTGNSAVL
                     LGTVTSGSGEPLGDGQISQTLTLSAGSTPTLSFWEWPLTTDSVTFDQQYVRVQPINPP
                     GPVVVLMNEARNDQTFLHRTFSLSQFAGSTVKLIFGVHQDGFGDITGMFVDDISVTSQ
                     NCGPPDFKVSVTPVTGTEVCAGQSLQFSVAVNSVNGPNFTSPVTLSAGNLPPGATATF
                     ASNPVSPGGSTIMTLTTARPTTGSQFTINVNGVAVTPPPTGTRTASTPVLVDPNAPNA
                     PEIISPAIGAVNLPRRPTLSWTSPFVPDGSATSGSSTSGSSASSPGSVSSSGAAPAPT
                     GSQPGMFAWQLAGTMAPLGSASAVTPFAFGASSYHLQIARDAAFSNIVVDTTTSTTSF
                     TVGFD"
BASE COUNT          966 a         2200 c         1917 g          890 t
ORIGIN      
        1 cggatagccg agcgtgaaca tgtcggagtc cgaccggatc cccggatagc gaaacagatc
       61 ccaggtcccg ccgatcgcgt cgcgcccctc caggatcgcg aaccgcttgg tcggacactc
      121 ggtctgcagg cggtacccgg caccgacgcc ggacagccca gcgcccacga tgatgacatc
      181 gagatcatcg ccctgatgag aagcacgctc cggagccatg cgcgtagcct actgcagcgc
      241 ggcgagcgcg tcaggattga cctgcggtca attggcttcg gtggttcgac ctgcgccgct
      301 ggcaccttga ggtgatcgcg agctttcctc caggcgccac gcggggaacg acgaagatga
      361 tccgaggtcg acggcgacgt cgaaccgtcg acgtcaacca ctgcttgcgt ccggatcgcc
      421 cctctctctt ccttcgacac ctgctgaacg ctcgggcgtg tgggggccag ggtgtatggt
      481 gcctcgccat ggcacccctc ggatcgcgca tccccatcct cgggccagtc ctcggcctgc
      541 tcctcgccgt gcccgcctgc tcgtcgtccc cggcgcatcc gccggcgagc gcggcggccc
      601 cggcgagtgg tgagccttca tcgccgccgg ccggctcgcc ccagggcgcc accccgccgg
      661 cggcccagcc ggtcgacccg gcggcgtcgc ccgaccaggc gttcgcggcg ttcgcccagc
      721 acttcctcga cgagatgctg cggcgatcgc cgaccttcgc gacccacgcc ggcgaccacc
      781 gccacgacga tcgctggccc gacgtctcgg tccagggcca ggccaccgac cgctcgttcc
      841 tcgaagagac ccgggccgcg ctcgcccggc tgccgcgcga gcggctgtcc gagcagaacc
      901 agatcgacgc cgcgatcctc gacgagcggc tgcgcgccac gctgttctcg ctcgaagagc
      961 tcaagccgta cgacaccgat ccgatggcct acgtcggcct gatcagcaac gggatcgatc
     1021 cgctggtcac ccgcgagttc ggcacccgcg agtcgcggat ggccagcctc gcgggacggc
     1081 tcgacggcat ccccgcgatc gtggcggtcg ccaggcagcg cctcgcccac gccgcgaagg
     1141 tgttcaccga gaccgcgatc cagcagaaca agggcctgat cgcgctggtc gagacccagc
     1201 tgccggcgcg gttcgccgag gtccccgccc agaaggaccg gctcacggcc gccgcggccc
     1261 gcgccgcgac cgcgctccat gatctgcagc gcttcctcga gaccgacctc ctgccgcgct
     1321 ccgacggctc gttccggctc ggccgcgagc ggttcgccag gaagctcgcg ttcgcgctcg
     1381 gcgaggacgt cgacatcgac accatcgccg ccgacgcccg cgcgctgctg acccggatcc
     1441 aggccgagat ggtcgacacc gcccggcaga tctgggccga cgacaagctc ggcaagctgc
     1501 cgccgctcga caccgccgag cagaagaagg cgttcgtcaa gcgcgtgctc gaccacgtcg
     1561 cggccgaccg cccgaccaac cagacgatcc tcgccgacgc gcggcgctgg ctcgacaagg
     1621 cgaccgcgtt cgtccgcgat cagaacctcg tgcgcgtccc cgacgagccg gtcgcggtga
     1681 tcgagatgcc cgagtacaag cgcggcgtcg cggtcgccta ctgcgactcg tccggcgcgc
     1741 tcgaggccac gccgcagacg ttcttcgcga tctcgccgac gccgtcggac tggcccaggg
     1801 cgcgcgccga gtcgttctat cgcgagtaca accaggcgat gctcgccgat ctgacgatcc
     1861 acgaggcgat gcccggccac tacctccagc tcatgcacaa taaccagttc gcgtccaagc
     1921 tgcgcgcggt gttcgccagc ggcccgttcg tcgagggctg ggcggtctac gccgagtgga
     1981 tgatggccga gcacggcttc ggcggcccac gggtcaagct ccagcgccag aagatgatgc
     2041 tccggacgtg cgccaacgcg atcctcgatc acgacatcca cgccggcacg atggacgagc
     2101 aggccgccct ggcgctgatg aagaacgagg cgttccagga ggacggcgag gcggtcggca
     2161 agtggaagcg cgcccggctg tcgagcgcgc agctcaccac gtacttctac ggcttctccg
     2221 agctgctcaa gctgcgccgc gccgtcgaat cccagcccgg gttctccgag cgcgcgtatc
     2281 acgaccgcct gctgtcctgg ggctcgccgc cgatgaggta tgttcgtcag ctggtcgccc
     2341 gacactgacc ccgggagggg cgggagcggt gtcgcgtttg ccggtaaatg ccacttttgc
     2401 cgggacgctg gcgcccggtc gatgagcgcc ggcgctcggt gcccgaacgg tgtgcgcctg
     2461 tacacgcgtc tgaggatacc tgcgcggcca tcttgagaat gaaaatcaat cccagccccc
     2521 actcccgtct cgccggttcg gagcgacgac accccccgcg gcgccgtcga ggcgagacga
     2581 tgccgaccca tgtctcgcaa cggcaacatc acaatgaggg tcccgcgagc atcccgatct
     2641 ccattggatt ggtttgtaac ggcagctacg cgaaaaattc gggagttcag ctcgagcgtt
     2701 ccgcggatcg tgctggagat ccggagtccc gcgaagtgag gaatcgccgc gactcgctca
     2761 ctggattgcg gccgtcgagt cgctcacagc ttcgtgaggg tgatctcggc gctgctcccg
     2821 gcaagggcga gttcacggga tcgggctcgc gccgcccgtg cctgggcttg ccaccggccg
     2881 gtgcgacctg cgacgcaaca gaaattcacg acgagatcgg ccgcgagatc gatggcgcgg
     2941 cacaaccgtt cggtataagg tgcaggtctc gcactcggca gtcatcggct gagaccggca
     3001 gtgccctgcg tgtatgggcg ttatttgctc ctggtaacca ctcttggcaa tttggttgta
     3061 ggctctctat tagccattga ttaagccttc cacgagtgat attgctaccc gggttccccg
     3121 cacgccctga agtccagggc tgcagacatt cggtcgcagg cgcactcgag gagggcatcc
     3181 atggtatttt cgtcagcatc gcgcacgatg gtcgccgcat tcgcgacgct cggcgcagtc
     3241 ggttgcgcga cgccgcaatc cgacacatcc acagtcgaga gtaaggagtc ccaggccggg
     3301 atgttcttac acggacaggc acggcaggat aagccgagtc cgttcaccca tccggcggga
     3361 gcacatctcc agtatttcgg cggccggatc atgtccaaca tccaggtcgt ccaggtgctg
     3421 tacggcaccg gcagctacat cccgcaggtc acgagcacgt cgacgcccag catggcgacg
     3481 ttctaccagg gcgtgctcaa cagcccctac gtcgactggc tgacggagta caacacggtc
     3541 gggctgcccc cgcccagcag caaccaggtg ctgggccggg gctcgttctc ccagcaggtc
     3601 cagatcaccc cgaacccggc caacaacggc acgctgatcg acgacctcca gatccagaac
     3661 gagctgtccg cgcagatcgc cgccggtacg ctccccgctc ccacgcatga cccggcgggc
     3721 aacaacaaca cgtactacgc gctcttcttc ccgcacggga aggtgatctc gctgggcggc
     3781 tcgctgtcgt gccaggtgtt ctgcgcctat cacggcacga tcgccagcgc cggcggacag
     3841 ggcgagatcg agtacggcgt gcaccccgac ttccagcccg gctcgggttg cgagaacggc
     3901 tgcggcgcgg ccgcgaccca gttcggcaac tacacccagg tcgcctcgca cgagctgatc
     3961 gagaccatga ccgatcccga ggtcggcctg gcgaccgtgt tcggtccgcc gctggcgtgg
     4021 gccgacaccg tgttcagcga gatcggcgac atctgcaacg accagaacgg tcaggtggcc
     4081 ggtgcggacg gcgtcaccta cgacgtccag accgagttct cgaactcggt caatgactgc
     4141 atcgtgatca accccgccgc cacgccggtc accgcgacca gcgcgggcga ggcctgccgc
     4201 ggcaccaccg cgctggcgac ggtcacggtg ctcggtggcg ccggccggtt caccagcccc
     4261 gtgacgctca gcgtcaccgg cgtgagtccg acgccgccgc ccggcggcga gatcaccgcg
     4321 acgttcgatc ccgacccggt gccgacgccg ccggtcaacg gcgcgacgtc gacgatgcgg
     4381 atctcgagca cgccgctgac cccgcccggc acgtacacgc tgacgatcca ggcggcgagc
     4441 acgggcctga cccggacgac cacgacgcag gtcgtgatcc gctcggaggt gccgaacggc
     4501 ccgacgctga cctcgccggt caacggcgcc gacggcgtgg cgcaggccgc gaccttcacg
     4561 tggtcgccgg tcgaccaggc cacgcagtac gagctcgacg tgttcgcggg cagcgactgc
     4621 accggcacgc cggtccggac cttcaccacc ggcaacacca acttcacgat tccccaggcc
     4681 caggcgctgg acacgttcca gaacttctcg tggcacgtgt cggccgccaa ctcgtgcggc
     4741 agcagcgcga cctcggcgtg cttcaacttc cgcaccgcct cgtgctccga tccgatcgag
     4801 gcggtcacca acggcgggtt cgagaacggc ctgccgggct ggacgaccga gctcgcggtc
     4861 ccgccgccgg tcgtgagctc cgcgcacgtc cacaccggaa acagcgccgt gctgctcggc
     4921 accgtgacct cgggcagcgg cgagccgctc ggcgacggcc agatctcgca gaccctgacc
     4981 ctgtcggccg gcagcacccc gacgctgtcg ttctgggagt ggccgctgac caccgactcg
     5041 gtcacgttcg accagcagta cgtccgcgtc cagccgatca acccgcccgg cccggtcgtc
     5101 gtgctgatga acgaggcgcg caacgatcag accttcctcc accggacgtt cagcctgtcg
     5161 cagttcgccg gatcgacggt caagctgatc ttcggcgtcc accaggacgg cttcggcgac
     5221 atcacgggca tgttcgtcga cgacatcagc gtgacgtcgc agaactgcgg tccgccggac
     5281 ttcaaggtct cggtcacccc cgtcaccggg accgaggtgt gcgccggcca gagcctgcag
     5341 ttctcggtcg cggtcaactc ggtcaacggc ccgaacttca cctcgccggt gacgctcagc
     5401 gccggcaacc tgccgccggg tgccaccgcc acgttcgcga gcaacccggt cagcccgggc
     5461 ggctcgacga tcatgacgct gaccacggcg cggccgacca ccggcagcca gttcacgatc
     5521 aacgtcaacg gcgtggcggt caccccgccg ccgaccggga cgcgcaccgc ctcgacgccg
     5581 gtgctggtcg atcccaacgc gcccaacgcg cccgagatca tcagcccggc gatcggcgcg
     5641 gtcaacctgc cacggcggcc gacgctgtcg tggacctcgc cgttcgtgcc cgacggcagc
     5701 gcgaccagcg gctcgtcgac ctcgggctcg tcggcatcgt cgcccggctc ggtctcgtcc
     5761 tccggtgcgg cacccgcccc gacggggtcg cagccgggca tgttcgcctg gcagctcgcg
     5821 gggaccatgg cgccgctggg cagcgcgagc gccgtgacgc cgttcgcgtt cggcgcgtcg
     5881 tcctaccacc tgcagatcgc gcgtgacgcg gcgttcagca acatcgtggt cgacaccacg
     5941 acctcgacga ccagcttcac cgtcgggttc gac
//
LOCUS       DASVOR010000270         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_470127,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000270 DASVOR010000000
VERSION     DASVOR010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470127"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2822)
                     /locus_tag="VF516_05705"
     CDS             complement(<1..2822)
                     /locus_tag="VF516_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8107202.1"
                     /translation="MKLIKLGTFVATGLLVGFLAKQPEPVVGDLPNRMPEAAPYITDE
                     ARIFPPPYEEYWKTQNHPGQCQTCHQKIFDEWNGSMMSNSWRDPVWRGAYLLLARTTS
                     TNGECDTPTPPDGTEKSHHNPFAKPGECASVFDIGAERVTLSRPGSLLDAFCSRCHMP
                     TNYLDNIPLRNITMDKFGLEHAPADLHFNPTSDNHTGIAFATVEEQFRNTESGKTGIF
                     CAICHTFAATRDTPFHNYTRSGTEYTPAVGTESRADLVRDTQDLFKVADITKPNLGYS
                     IGAGAYRLSPHAIVFPERFGPMAATAAPEPSDTNTSKVFGENIAYQQVDSSKHKAYHS
                     SMFVRSEMCAACHDVTNALPIKNSLGRYAGGFPIERTYTEWRNSRYAERPDNKFYEPA
                     HKRDCQSCHMQQDYGQPGTAQTLYDPAGHPLPILKEPVATDGKERPFFTHHFVGGNTF
                     VPRLIGKDVDNSGNVSPYPELSVYSYSSDDHNSPYSRAVWTHTERKGVYAQQARLAWD
                     RLRHAVELQSSGPATAAAGTTAPISLTVQNTGCGHDFPSGFPEGRIAWIAVHAYDLAT
                     GKELMIEDSEPAFKDKDGKPRRSLGVGNLTTEDVVDPNYPKCTAAGKPWILPPGSPDP
                     WSIQFKAVASLGDGCPTLDLPFATPLNLKVNDQNLPVDASGKVIDSTHNPTGLEQFSD
                     VDGDGEFFDDSFLVDTRLRPLPHPGATKKADRYSVVIPPGTRGPIAVSTAIYYQSMEA
                     IVGQKFLGNMTDSNNNMVIEPCVLGGRCDGRKPRTEPAVVEGAPPVPVAVKNFTIAIA
                     GAAPDATPPRVAVYPSPGAKRAHLDSVPKVFFSRPVTGVTAATFTVTDASGAVQPAWV
                     DQIGDGTWGLFANQIQYKPGATYTAHLAAGICAADAPTACTTEASEWSFTIAADADTS
                     DGDSSVPMGFSGPKVAPAPMRAAAAPA"
     gene            complement(2836..>3469)
                     /locus_tag="VF516_05710"
     CDS             complement(2836..>3469)
                     /locus_tag="VF516_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107203.1"
                     /translation="LSSYDWLISLKPKWFSTMFSVNIFAGAFVETIAAIALATVVLKR
                     RQLWGKDGALIDMNLIRTLGTMMLAFSTFWGYTWICQYLLVWYSNIPDEAAWYVPRSS
                     GGWLPLLIASFVVCWIIPFFTLLPFKSKSSLKVMTVMAILILAGRWFDLYMQVMPSHW
                     DSPRFGWIELGMAAGTAGLIWLVFVRVLSRAPLIPPHEPVLEARRGQAHH"
BASE COUNT          509 a         1088 c         1283 g          589 t
ORIGIN      
        1 gccggagccg ccgcggcgcg catcggagcc ggcgcgacct tcgggccgga gaatcccatc
       61 gggacgctgg agtcgccgtc cgaggtgtcg gcgtcggcgg cgatcgtgaa gctccactcg
      121 gacgcctcgg tcgtgcaggc ggtcggggcg tccgccgcgc agatcccggc ggcgaggtgc
      181 gccgtgtagg tcgcgcccgg cttgtactgg atctggttgg cgaacaggcc ccaggtgcca
      241 tcgccgatct ggtcaaccca cgccggctgc accgcgccgc tggcgtcggt gacggtgaag
      301 gtggcggcgg tgacgcccgt gaccgggcgc gagaagaaca ccttgggcac gctgtcgagg
      361 tgggcgcgct tggcgccggg ggacgggtag accgcgacgc gcggcggggt ggcgtcgggc
      421 gcggcgcccg cgatcgcgat cgtgaagttc ttgaccgcca ccggcaccgg cggggcgccc
      481 tcgacgacgg ccggctcggt ccgcggcttg cggccgtcgc agcggccgcc cagcacgcac
      541 ggctcgatca ccatgttgtt gttgctgtcg gtcatgttgc cgaggaactt ctggccgacg
      601 atcgcctcca tcgactggta gtagatcgcg gtcgacactg cgatcgggcc acgcgtgccg
      661 ggcgggatga cgaccgagta gcgatcggcc ttcttggtcg cgccggggtg cggcagcggc
      721 cgcagccggg tgtcgaccag gaacgagtcg tcaaagaact caccatcgcc gtcgacatcg
      781 gagaactgct cgaggccggt cgggttgtgg gtcgagtcga tcaccttgcc gctggcgtcg
      841 accggcaggt tctggtcgtt gaccttgagg ttgagcgggg tggcgaacgg cagatccagg
      901 gtcgggcagc cgtcgccgag cgacgcgacc gccttgaact ggatcgacca cgggtcgggc
      961 gagccgggcg gcaggatcca cggcttgccc gccgccgtgc acttgggata gttcgggtcg
     1021 acgacgtcct cggtggtgag gttcccgacg ccgagcgacc tccgcggctt gccgtccttg
     1081 tccttgaacg ccggctccga gtcctcgatc atcagctcct tgccggtggc gaggtcgtag
     1141 gcgtggaccg cgatccaggc gatccggccc tcggggaagc cgctcgggaa gtcgtggccg
     1201 cagccggtgt tctggacggt gagcgagatc ggggcggtgg tgccggcggc cgcggtggcc
     1261 gggccgctcg actgcagctc gaccgcgtgg cgcaggcggt cccacgccag gcgcgcctgc
     1321 tgggcgtaga cgcccttgcg ctcggtatgg gtccacaccg cgcgcgagta cgggctgttg
     1381 tggtcgtccg acgagtagct gtagaccgac agctcggggt agggcgacac gttgccgctg
     1441 ttgtcgacgt ccttgccgat caggcgcggc acgaacgtgt tgccgccgac gaagtgatgc
     1501 gtgaagaacg gccgctcctt gccgtcggtc gcgaccggct ccttgaggat cggcagcggg
     1561 tggccggccg ggtcgtacag ggtctgcgcc gtgccgggct gaccgtagtc ctgctgcatg
     1621 tggcacgact ggcaatcgcg cttgtgcgcc ggttcgtaga acttgttgtc cgggcgctcg
     1681 gcgtagcggc tgttgcgcca ctcggtgtag gtgcgctcga tcgggaaccc gccggcgtag
     1741 cggccgagcg agttcttgat cggcagcgcg ttggtcacgt cgtggcacgc cgcgcacatc
     1801 tcggatcgca cgaacatcga cgagtggtag gccttgtgct tggacgagtc gacctgctgg
     1861 taggcgatgt tctcgccgaa caccttgctg gtgttggtgt cgctcggctc cggcgcggcg
     1921 gtcgcggcca tcgggccgaa ccgctcgggg aacacgatcg cgtgcggcga caggcggtac
     1981 gcgccggcgc cgatgctgta gccgaggttg ggcttggtga tatcggcgac cttgaacagg
     2041 tcctgcgtgt cgcgcaccag gtcggcgcgc gactcggtgc cgaccgccgg ggtgtactcg
     2101 gttccgctcc gcgtgtagtt gtggaacggg gtgtcgcggg tcgcggcgaa ggtgtggcag
     2161 atcgcgcaga agatgccggt cttgccggac tcggtgttgc ggaactgctc ctcgacggtc
     2221 gcgaacgcga tgccggtgtg gttgtccgac gtcgggttga aatgcaggtc ggccggtgcg
     2281 tgctccaggc cgaacttgtc catcgtgatg ttccgcagcg gaatgttgtc gaggtagttg
     2341 gtcggcatgt ggcagcgcga gcagaacgcg tcgagcagcg agccggggcg cgacagcgtc
     2401 acgcgctcgg cgccgatgtc gaacaccgac gcgcactcgc ccggcttggc gaacgggttg
     2461 tggtgcgact tctcggtgcc gtcgggcggc gtcggggtgt cgcactcgcc gttggtcgac
     2521 gtcgtgcgcg cgagcagcag gtacgcgccg cgccacaccg gatcgcgcca cgagttcgac
     2581 atcatggagc cgttccactc gtcgaagatc ttctggtggc aggtctggca ctggcccggg
     2641 tgattctggg tcttccagta ctcctcgtag ggcggcggga agatcctggc ctcgtctgtg
     2701 atgtacggcg cggcctcggg catccggttc ggcaggtcgc cgaccactgg ctcgggctgc
     2761 ttggcgagga atccgaccag gagccccgtg gcgacgaacg ttcccaactt gatgagcttc
     2821 acgacgctcc tcctttcagt gatgcgcttg accgcggcgc gcctcgagca ccggctcgtg
     2881 gggcgggatc agcggcgcgc gcgagagaac tcgaacgaag accagccaga tcagcccggc
     2941 ggtgcccgcc gccatcccga gctcgatcca cccgaaccgc ggcgagtccc agtgcgacgg
     3001 catcacctgc atgtagagat cgaaccaccg gccggcgagg atcaggatcg ccatcacggt
     3061 catcaccttc aggctgctct tggacttgaa cggcaggagg gtgaagaacg ggatgatcca
     3121 gcaaaccacg aagctggcga tcaagagcgg cagccagccg ccggagctgc gcgggacgta
     3181 ccacgccgcc tcgtccggga tgttgctgta ccagaccagg aggtactgac agatccacgt
     3241 gtagccccag aacgtcgaga acgcgagcat catcgtgccc agggtccgga tcaggttcat
     3301 gtcgatcagc gcgccgtcct tgccccacag ctgcctgcgc ttgagcacca cggtggcaag
     3361 cgcgatcgcg gcgatggtct cgacgaacgc gccggcgaag atgttgaccg agaacatcgt
     3421 gctgaaccac ttgggcttga gcgagatgag ccagtcatag gacgacagc
//
LOCUS       DASVOR010000271         2593 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_323958,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000271 DASVOR010000000
VERSION     DASVOR010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2593
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323958"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VF516_05715"
     CDS             complement(<1..799)
                     /locus_tag="VF516_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX8107204.1"
                     /translation="MEAVVSPLPDDIEALKALLASATQRANEAEAKLANAHARESATE
                     AVIAHLKLQIAKLRREQYGASAERTRRLLDQMELQLEELEADAAEDDLAAEVAAEKTA
                     TVTAFERKRPARKPFPEHLPRERVVVPAPCSCPACGSSRLSKLGEDVTETLEVIPRAW
                     KIIQTVREKFSCRDCETITQPPAPFHVVPRGWAGPSFLAMLLFEKYGQHQPLNRQAER
                     FAREGVPLSTSTLADQVGAATFALMPLYRLIEAPVLAAERLHGDDTTV"
     gene            complement(872..1213)
                     /gene="tnpB"
                     /locus_tag="VF516_05720"
     CDS             complement(872..1213)
                     /gene="tnpB"
                     /locus_tag="VF516_05720"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX8107205.1"
                     /translation="MIPSGARVWIAMGHTDMRKGMQGLALLVQQGLKRDPHGGDLFVF
                     RGRAGSLVKLIWHDGIGMSLYAKRLEKGRFVWPSAKDGMVSLTASQLACLLDGIDWRN
                     PQYSWRPQSAG"
     gene            complement(1210..1578)
                     /locus_tag="VF516_05725"
     CDS             complement(1210..1578)
                     /locus_tag="VF516_05725"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8107206.1"
                     /translation="MGQITVFSGPERRRRWSDEERLEILSEAFAPGACVAEVARRRDV
                     STALVYTWRRKLRDAHAEPEPDDLAAPDFAAAVMIEDVEAAHPGLQPAIVIDLARGKR
                     ISIFASASPALVASALKALR"
     gene            1714..>2593
                     /locus_tag="VF516_05730"
     CDS             1714..>2593
                     /locus_tag="VF516_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX8107207.1"
                     /translation="MEHSTEVFVGMDVAKTRNAIAVADGERGGEVRFLGEVDASPEAM
                     RRVVQRITAKHERVHFCYEAGPTGYGLHRLITSMGYPCDVVAPSLIPRRPGDRVKTNR
                     RDAIGLAKLLRAGELTSVWVPDEGHEAMRDLVRARAAAVETQRVHRQQVNAFMLKHGR
                     IFPRKTTWGARHLRWLQEQRFDHPAHQIALQELVDAVRTSRERIVRIEAAIEEFLPSW
                     SLAPVVRALQALRGVELVVAVTFVTEIGDVRRFDSPRQLMGYLGLVPSERSTGDAIRR
                     GGITKAGNGRVRHMLVE"
BASE COUNT          461 a          824 c          865 g          443 t
ORIGIN      
        1 gcacggtcgt atcgtcgcca tgcaatcgct ccgcggcgag gacaggggcc tcgatcaagc
       61 ggtagagcgg catcagcgca aaggtggcgg caccgacctg gtcggcaagc gtcgaggtgc
      121 tcagcggcac gccctcgcga gcgaagcgct cggcctggcg gttgagcggc tggtgctgtc
      181 cgtacttctc gaacagcagc atggcgagga agctgggacc agcccagcca cgcggcacga
      241 cgtggaaggg cgccggcggc tgtgtgatcg tctcgcagtc ccggcaggag aacttctcgc
      301 gcacggtctg gatgatcttc caggcgcgcg ggatcacctc gagcgtctcg gtaacgtcct
      361 cgcccagctt ggacagccgg ctgctgccac aagccgggca agagcacggc gccggtacga
      421 cgacccgctc gcgcggcagg tgctcgggaa acggcttcct ggccggccgc ttgcgttcga
      481 aggcggtgac ggtagcggtc ttctcggccg cgacctcggc agcaagatcg tcctcggcgg
      541 cgtcggcctc gagctcttcg agctgcagct ccatctggtc gagcagtcgg cgggtgcgct
      601 cggcgctggc gccatattgc tcacgcctga gcttggcgat ctgcagcttg aggtgcgcga
      661 ttaccgcctc ggtggcgctc tctcgggcat gggcattggc gagcttggcc tcggcctcat
      721 tggcccgctg ggtcgcgctc gccagcagcg ccttgagcgc ttcgatatcg tcaggaaggg
      781 gcgaaacgac ggcttccatg accgcgatgg aatcacagaa acccgtgcga atgaagcgca
      841 aaatgctccg ctggtgaaga aagcgacgcc cctatccggc gctctgcgga cgccaggaat
      901 actgcgggtt ccgccagtcg atcccgtcga gcaggcaagc cagttgcgag gctgtcagcg
      961 acaccatccc gtccttcgcc gatggccaca cgaaccgccc cttctcgagc cgcttggcat
     1021 agagcgacat gccgatcccg tcgtgccaga tcagcttcac tagcgatccg gcgcgcccgc
     1081 ggaatacgaa cagatcgccg ccgtggggat cgcgcttgag gccctgctgg accagcaaag
     1141 ccaggccctg catccccttg cgcatatcgg tgtggcccat cgcgatccat acccgggcgc
     1201 ccgaagggat caccgcaggg ccttcagtgc cgaggccacc agcgccggcg atgccgaggc
     1261 aaagatgctg atccgcttcc cgcgcgccag gtcgatgacg atcgcgggct gtagaccggg
     1321 gtgagcggct tccacatcct cgatcatcac ggcagcggcg aaatcgggag cggccaaatc
     1381 gtccggttcg ggctcggcgt gagcatcgcg cagcttacgc cgccacgtgt agaccagggc
     1441 cgtcgataca tcgcgccgcc gtgcgacctc cgcgacgcag gccccgggcg caaaggcctc
     1501 gctcaggatc tccagccgct cctcgtcgct ccagcgtcga cgtcgctccg gcccggaaaa
     1561 caccgtgatc tgccccatcg atcgctccta agcgcactct taagagcgta agaccgtatg
     1621 tccgctcttt ggctgagggt ggctcgccct acactgtggt ggcgagggtt gctcggagtg
     1681 gccgagccga tcctcattgc aggaggacga gccgtggagc attctacaga agtcttcgtc
     1741 ggaatggatg tggcaaagac gcggaacgcg attgcagttg cggatggcga gcgcgggggc
     1801 gaggtgcggt tcctgggaga agtcgacgcc tctcctgagg caatgcgacg ggtggtccag
     1861 cggatcaccg caaagcacga acgtgtccat ttctgctacg aagccgggcc gaccggctat
     1921 ggcctccacc gattgatcac atccatgggc tatccttgcg acgtcgtcgc accttcactg
     1981 ataccgaggc gaccgggcga cagggtgaag accaacaggc gcgacgcaat cggcttggcg
     2041 aagctcttgc gcgccgggga gttgacgtcg gtgtgggtcc ccgatgaagg acatgaagcc
     2101 atgcgcgatc tggtccgggc acgcgctgcg gcggtcgaga cccagcgcgt gcaccgacaa
     2161 caggtgaacg cgttcatgct gaagcacggg cgcatctttc cgcgcaaaac gacatggggc
     2221 gcacgccatc tccggtggtt gcaagaacag cggttcgatc atcccgctca ccaaatcgca
     2281 ctgcaggaac ttgtggatgc ggttcgcacg tccagggagc gtattgttcg catcgaagcg
     2341 gcgatcgaag agttcctgcc cagttggtca ctggcaccgg tcgttcgcgc gctccaggcc
     2401 ctaagaggcg tagagcttgt cgtcgctgtc accttcgtca ccgagattgg cgatgtgcgt
     2461 cggttcgaca gtccgcgcca gttgatggga tatttgggct tggtgccgag cgagcgttcg
     2521 actggcgacg caatccggcg tggcgggatc accaaggccg gcaatggaag agtccgacac
     2581 atgctggtgg aaa
//
LOCUS       DASVOR010000272         4833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_323959,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000272 DASVOR010000000
VERSION     DASVOR010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4833
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323959"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..667)
                     /locus_tag="VF516_05735"
     CDS             complement(98..667)
                     /locus_tag="VF516_05735"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX8107208.1"
                     /translation="MHGREAGSTLEAGFTLVEMLVALAVISILATIALPSFFSETRKT
                     RARAEVTPMFNDLRVRMEQYLQERGVYPDTLGETGLYPTSSPDATLRPLQPLPATWTA
                     LNIRISGPDQVRCGYTWVTSRSGNGTVGPTAANAGAKAMARPFSFAPPDNDWYYLLAK
                     CRMDPGHSGYSWYFTSSLDSAIKSDNEGQ"
     gene            complement(708..1238)
                     /locus_tag="VF516_05740"
     CDS             complement(708..1238)
                     /locus_tag="VF516_05740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8107209.1"
                     /translation="MTPSRQAQRGFTLIELMIVVAIIGILAAVAIPAFMDYMKRSKKT
                     EASLQLNKIGKNSKRTYMETSKYVTGVAPTTPNRPGRGGCCDGLGASGSNTNHCAPDV
                     SPAGFGHPVWTSLDFSIEEDSLFYYDYNGTATDYTAMATGDLDCDGTEITYVLTGTAI
                     NGQPAYSLSEPPSSDD"
     gene            complement(1440..1979)
                     /locus_tag="VF516_05745"
     CDS             complement(1440..1979)
                     /locus_tag="VF516_05745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8107210.1"
                     /translation="MKLTKQAKRGFTLIELMIVVAIIGILAAVAIPAFMDYMKRSKKT
                     EASLQLNKIGKNSKRAYMETSSYVTGSVGTTPAKPGGGVGGGCCGGSGTSGKNTNHCA
                     AAPTSFTGDPVWTALDFEIDEDTLFVYDYNGSSTTTYVAHAVGDLDCDKTEITYVLTG
                     TAVNGQPAYELSEPPSSAD"
     gene            complement(2274..2786)
                     /locus_tag="VF516_05750"
     CDS             complement(2274..2786)
                     /locus_tag="VF516_05750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8107211.1"
                     /translation="MNKIQRGFTLIELMIVVAIIGILATVAIPAFMDYVKRSKKSEAT
                     LQLDKIGKNAKRTYVETGSFPVGSAGPTPARPGTGGCCGGVGTSGKNINHCAANPAAW
                     TGVWTQLEFEIDEDSLFYYGYTGAAQSFTATGIGDLDCDGREVTYTLNGTTVNGNPIA
                     TISEPPPNSD"
     gene            complement(2946..4442)
                     /locus_tag="VF516_05755"
     CDS             complement(2946..4442)
                     /locus_tag="VF516_05755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8107212.1"
                     /translation="MGKILVVDDEQSMREFLGICLRRAGHEVTLAQTGGEAIAQLRSQ
                     PIDIVVSDLKMPGEIDGLNLLQQIKAGAIRRAAVPGANPAPIDPEVILVTAFATTDTA
                     IAAMKQGAYDYLTKPFQVDEINAVIGRALEKRALVEDNLALRDQLAGRVRLAQLLGKS
                     RAMQKVFELITKIHSVRTSVLITGESGTGKELVARALHSEGSRARAPFIAVNCGAIPE
                     ELMESELFGHKRGAFTGAIADKIGMFQEADGGTLFLDEIGELSLGLQVKLLRALQERR
                     VKPVGATEELEVDVRVIAATNRDLETEVSRGAFRADLYYRLNVIEIRLPPLRHRREDI
                     PLLAEHFLRRFSAEHHRASKLSAEAMRRLESYEFPGNVRELENIIERAVALSSSALIG
                     ASDLPEVRTPRIAAPQPPSALPPEGVDLDQLVSDYERTWVLRALEQTGGVRKRAAALL
                     GISFRSLRYRLEKLGIDRGDDRGDDRGGAGGDAGGDDRGDDKPEDDLE"
     gene            4553..>4833
                     /locus_tag="VF516_05760"
     CDS             4553..>4833
                     /locus_tag="VF516_05760"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEX8107213.1"
                     /translation="MGRLTPRVLLIDRGGLIALAALVGYVWLAPTYIVDGDNAEFATL
                     GTVGGVAHPTGYPAYLLWLRATAWLPGAAPAHTAAIATAILAAIQLAVL"
BASE COUNT          679 a         1630 c         1693 g          831 t
ORIGIN      
        1 catgcgccgc gggccgcggg ccgccgggcc ggcgtccgcg gtcacgggcc ctcgatcacg
       61 ggccctcgat cacgggccct cgatcacggg ccctcgatca ctggccctcg ttgtccgact
      121 tgatcgcgga gtcgaggctg ctcgtgaagt accagctgta gccggagtgg cccggatcca
      181 tgcggcactt cgccagcagg tagtaccagt cgttgtccgg cggcgcgaag ctgaacggcc
      241 tcgccatcgc cttggcgccc gcgttcgccg cggtggggcc gacggtgccg ttgcccgatc
      301 ggctggtgac ccaggtgtag ccgcagcgga cctggtcggg gccgctgatc cggatgttga
      361 gcgccgtcca ggtcgccggc agcggctgga gcgggcgcag cgtcgcgtcg gggctgctcg
      421 tcgggtacag cccggtctcg cccagcgtgt cgggatacac gccgcgctcc tggaggtact
      481 gctccatccg gacccggaga tcgttgaaca tcggcgtgac ctcggcgcgc gcgcgcgtct
      541 tgcgggtctc gctgaagaag ctcgggaggg cgatcgtggc gaggatcgag atcacggcga
      601 gcgccaccag catctcgacc agcgtgaatc ccgcttccag cgtggaccct gcttcgcgac
      661 cgtgcatgcc cgcagtgtag cgcaccggcc cgtcgtccgg aacgcgatca gtcgtcgctc
      721 gagggcggct cgctcagcga gtaggcgggc tggccgttga tcgccgtgcc ggtcagcacg
      781 taggtgatct cggtgccgtc gcaatcgagg tcaccggtgg ccatcgccgt gtagtcggtc
      841 gcggtgccgt tgtagtcgta gtagaacagc gagtcctcct cgatcgagaa gtcgagcgag
      901 gtccacaccg ggtgcccgaa gcccgccggg ctgacgtcgg gggcacaatg gttcgtgttg
      961 ctgccggacg cgcccaggcc gtcacagcag ccaccccggc cgggccggtt cggcgtggtc
     1021 ggggcgacgc cggtcacgta cttcgaggtc tccatgtacg tgcgcttgga gttcttgccg
     1081 atcttgttga gctggagcga ggcctcggtc ttcttcgagc gcttcatgta gtccatgaac
     1141 gcggggatcg cgacggccgc gaggatgccg atgatcgcga cgacgatcat cagttcgatg
     1201 agggtgaacc cccgctgggc ctgcctgctt ggtgtcatat gtggaacact gagcaacggg
     1261 tatgccgcgg cgaactgtcg agatctgcgc ttgcccgata ccaggctgtg tcgaagaatg
     1321 tcactcgcac gggtaaacag acgaggagac cactgtccca caggtcgtgc ggtgaacgcc
     1381 agcaaaaaaa cgccgccgga ccccagcccg gcggcgttca gacttcgcaa gcgaagtgat
     1441 cagtccgcgc tcgacggcgg ctcgctcagc tcgtaggccg gctggccgtt gaccgccgtt
     1501 ccggtcagca cgtacgtgat ctcggtcttg tcgcagtcga ggtcgccgac ggcgtgcgcg
     1561 acgtaggtcg tggtcgagct accgttgtag tcgtagacga acagcgtgtc ctcgtcgatc
     1621 tcgaagtcga gcgcggtcca caccgggtcc ccggtgaacg acgtcggggc cgccgcgcag
     1681 tggttggtgt tcttgcccga cgtgccgctg ccgccacagc agccaccgcc gacgccaccg
     1741 ccgggcttgg cgggcgtcgt cccgacgctg ccggtcacgt acgaggacgt ctccatgtac
     1801 gcgcgcttgg agttcttgcc gatcttgttg agctgcagcg aagcctccgt cttcttcgac
     1861 cgcttcatgt agtccatgaa ggcggggatc gcgacggcgg cgaggatgcc gatgatcgcc
     1921 acgacgatca tcaattcaat gagggtgaac ccccgcttgg cttgcttggt gagcttcatc
     1981 tcgtgtgcgc agagcaacgg atgtgccgcg tctaactatg taagatctgc gggcgtgaga
     2041 tgccgctacg tgccgaggaa tgtcactcgc gcggcgaaaa atggcctgcg gcgcggtctg
     2101 cggcctcaga aaatccacgg ttctgctcgg cgctctccgc gaggcggatc gcccagcgaa
     2161 cggcatgcca gcatacgagc gcagatatgg aacatgtcag acggtggacg agacctgcac
     2221 agcccggatg cccggcgagg cgcggtggtc cggccaccgg cgcgcgatcg cgttcagtcg
     2281 gagttcggcg gcggctcgga gatcgtggcg atcgggttac cgttcaccgt ggtgccgttg
     2341 agcgtgtacg tgacctcgcg tccgtcgcag tcgaggtcgc cgatccccgt tgcggtgaag
     2401 ctctgcgcgg ccccggtata gccgtagtag aacagcgagt cctcgtcgat ctcgaactcg
     2461 agctgcgtcc acacgccggt ccaggcggcc gggttggccg cgcagtggtt gatgttcttg
     2521 cccgacgtgc ccacgccgcc gcagcagccg ccggtgcccg gcctggccgg cgtcgggccg
     2581 gcgctgccga ccgggaagct gccggtctcg acgtacgtgc gcttggcgtt cttgccgatc
     2641 ttgtcgagct ggagcgtcgc ctcggacttc ttggaccgct tcacgtagtc catgaaggct
     2701 gggatcgcca cggtggcgag gatcccgatg atggcgacca cgatcatcag ctcgatcagg
     2761 gtgaatccgc gctggatctt gttcatgctc tgcatcgcca cgcctgctgg cggatcccgg
     2821 tgcagcaagc gccgcgccac cgagcgccga ggcccggggc gcacaacact gctgaagtgc
     2881 tcgttacccc agcgaatccg gagccgagaa ttgtcacccg gcgaccgccg aggtcgcgca
     2941 gaacgtcact cgaggtcgtc ttcgggcttg tcgtcgcccc tatcgtcgcc cccggcgtcg
     3001 cccccggcgc cgccccggtc gtcgccccgg tcgtcgcccc ggtcgatgcc gagcttctcc
     3061 agccggtagc gcagcgagcg aaagctgatg cccagcagcg ccgcggcgcg cttgcgaacc
     3121 ccgccggtct gttccagtgc gcgcagtacc cacgtccgct catagtcact taccagttgg
     3181 tccagatcta caccttccgg aggcagcgcg ctcgggggtt ggggtgccgc aattcgcggc
     3241 gtccggacct cgggcagatc gctcgcgccg atcagcgcgc tcgacgacag cgcgaccgcg
     3301 cgctcgatga tgttctcgag ctcgcgcacg ttgccgggga actcgtagct ctcgagccgc
     3361 cgcatcgcct cggccgacag cttcgacgcc cggtggtgct cggcggagaa ccggcgcagg
     3421 aagtgctcgg cgagcagcgg gatgtcctcg cggcggtggc gcaggggcgg cagccggatc
     3481 tcgatcacgt tgagccggta gtacaggtcg gcgcggaacg cgccgcgcga gacctcggtc
     3541 tcgaggtcgc ggttggtcgc ggcgatcacc cggacgtcga cctcgagctc ctcggtggcg
     3601 ccgaccggct tgacccggcg ctcctgcagc gcgcgcagca gcttgacctg caggccgagc
     3661 gacagctcgc cgatctcgtc gaggaacagc gtgccgccgt cggcctcctg gaacatgccg
     3721 atcttgtcgg cgatcgcgcc ggtgaacgcg ccgcgcttgt ggccgaacag ctcggactcc
     3781 atcagctcct cggggatcgc gccgcagttg accgcgatga acggcgcccg ggcccggctg
     3841 ccctcgctgt gcagcgcgcg cgcgaccagc tccttgccgg tgccggactc gccggtgatc
     3901 agcacgctgg tccggaccga gtggatcttg gtgatcagct cgaacacctt ctgcatcgcc
     3961 cggctcttgc cgagcagctg ggcgagccgg acccggccgg cgagctggtc gcgcagggcg
     4021 aggttgtcct cgaccagcgc gcgcttctcg agggcgcggc cgatcaccgc gttgatctcg
     4081 tcgacctgga acggcttggt caggtagtcg tacgcgccct gcttcatcgc ggcgatcgcg
     4141 gtgtcggtgg tcgcgaacgc ggtgaccagg atgacctcgg ggtcgatcgg cgccgggttc
     4201 gcgccgggga ccgcggcgcg ccggatcgcg ccggccttga tctgctgcag caggttcagc
     4261 ccgtcgatct cgcccggcat cttgaggtcg ctgaccacga tgtcgatcgg ctggctgcgc
     4321 agctgcgcga tcgcctcgcc gccggtctgc gccagcgtga cctcgtggcc cgcgcggcgc
     4381 aggcagatcc cgaggaactc gcgcatcgac tgctcgtcgt cgactacgag gatctttccc
     4441 acgcttccac tatggcacgg ctgccgcggc caccgcagta cgcgtggggc cgtacacggg
     4501 tgtcgcccgc ggcccgcggc ggcggtccgg gcggctgggg tatcgtagcc gtatgggtcg
     4561 gctcacgccg cgggtgctcc tgatcgatcg cggcgggctg atcgcgctgg ccgcgctggt
     4621 cgggtacgtg tggctcgcgc cgacctacat cgtcgatggg gacaacgccg agttcgcgac
     4681 gctcgggacg gtcggcggcg tcgcgcaccc gaccggctac ccggcgtacc tgttgtggct
     4741 gcgcgcgacg gcgtggctgc cgggtgccgc gccggcccac accgcggcga tcgcgaccgc
     4801 gatcctcgcg gcgatccagc tcgccgtgct gca
//
LOCUS       DASVOR010000273         6966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382358,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000273 DASVOR010000000
VERSION     DASVOR010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6966
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382358"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..3908
                     /locus_tag="VF516_05765"
     CDS             480..3908
                     /locus_tag="VF516_05765"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8107214.1"
                     /translation="MSNIQVTFDSSLNNARSESALVINPINPLQIVAASKKFNNIHTY
                     DFTLATAYSIDGGRSWQDSAPFTLPPGATVMTDPTLAWDDAGNVFSVGLVGNNPPEWD
                     TIGIAIYKSTDGGQTWGSPNVIHTSTGDDKQWMAGDGNQASPYHGRVYAVWDDGSVMR
                     FARTLDHGGSWIGTGTNSVASTSLANDSFAPEINVSANGDVYIVWIAGSTIKMLVSTD
                     GGDSFHGTTPPATGVTTLSSSLPNVGGFPVLPGGNFRVLTLPTACVYGKTVAVAWDDF
                     REGSSRIYRALSTDGGTTWSTGPSGQPLLTSAIDPALHHILPQIVADPAGVFGCAFYE
                     FGPKPTTPKIDVIMAQSFDGGATFDHFTVTDQPWDPVVDAPWSHGDSSVTFIGDYFGL
                     DASALGFYPLWTDTRTGIQELFTAIVPEKKCVFVVNRSTLGQDEVDARRALPGGAVIP
                     DAFRVVVDGFAATELGITGTGSTLPVASPAAGMTIVPRGNVSATLGYGPAVQRFTFFY
                     DIDFGATDLAFGFPGATRLVTLHVDAGPTSAEAQLELIKQPDPFLLHGDTFWLSIDLR
                     VFVVRAGESKFGVPGVADAGDAPRFIQQLISTVTPAQFDSLSAAEGASKLYLQPRDEN
                     GQLVFNFALAKVHYIGLIGATNVRVFFRLFQAQTTTGVFDYPPGAQYRRATGNPHGQP
                     IALPGIIGNEYVTIPCFAQPRVDSTVVSLNRQTDDHNVRPITAHGDGSEVDTFFGCWL
                     DINQPFAPDGVTPNNRLPVVVPGTHVDGPFTDPSNPPLPIQQAILRSLHQCLIAEIAF
                     DPVPIPVGKDPSNWDKLAQRNLAWSDVGSSEAVSTFEIRPTPLGQPASFLPDELMIDW
                     GDLPRGGIASIHVPAVSADEILAMAGRMYTLHGLVRADDHTLQCKTAGVTYLPIPPGS
                     GSAHAGVLSVELPDVLRREHVYKFVVRQVTNAYSRGVPPRQIESGRPEKEGVGSSATR
                     NFKLWRRVLGAFQLSIPVRSKQVLRVVEERDFAVLRWINQAIPSANRWHPVFRRYLEI
                     VSHRVRDFGGNPLQIPPSPTGDGAPRPPHPGGHGGITGMVEGLIFDHHGEFEGFILGD
                     PPHARRFYSREQKIEFIAERAWRERLRITVWAEHHEPHRPGTIVVHPPGHPLHR"
     gene            4138..5664
                     /locus_tag="VF516_05770"
     CDS             4138..5664
                     /locus_tag="VF516_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107215.1"
                     /translation="MADPIDVRLTALEARIAQLEGHLQVVTVAPPPAPPGDGAAALEA
                     RLGAYWLSRIGIVSLISGAALAILTYFAELGSVVRIALGYAAAGAIAWLGLRLARAHP
                     TFGRILFGGGLAVAYFVTYALHFVAALRVIDSEPLGIALVAAAIAAIVAIAHRMQSET
                     VAGIALFLGFHTGLLSEVTALSLITTTLLAAGAAFFLVANRWVIVPLSAVAAVYATHA
                     TLAFGNAAVGPGLRIAFVAIDFALFATALLIRSDTATRPRRLLALLNWLGALLLGGHA
                     AAAFSRDTLFLGLCGFAGAQLAIAAIARLRRAPGSAVTLELGLALVTLALALPVQLSA
                     GPLLAGWLALALAATAIARRAAPSFAVLALLLVLAGYGVVQVSHLGALAQLACVLAMI
                     AVERGHAPAARAAPLRTLAIAGVAAGLLQLAQITAPPGYHTLAWVGAGFVLFALGFAL
                     RTRAYRWSGFAALALAASRLIAVDVRRFSPDQRILTFVLAGIALLVVSFVYARREARG
                     "
     gene            5934..>6966
                     /locus_tag="VF516_05775"
     CDS             5934..>6966
                     /locus_tag="VF516_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107216.1"
                     /translation="MADTLSVGPGKTYAGIADAMPNAHPGDVIEVQGGNTYPGMIWLR
                     DGYGGTKDKPVTIRGIKVGGKRPVLSGVGSGQYENIILLLNSNHVVMESFEVIGDGGD
                     TNYCVVHKADDVILRDFVIHNCLHQGGHVGNDDGSGSLTLEYSELYHNGKGTDSHQIY
                     MATDETMYPGSVFRIQFCYVHDGLGGNNVRSRAERNEIYYNWVEGAFYHELDLVGPIG
                     GDSLPFREDSDIVGNVLIKKNSEWRIARLGGDGDGNTGGRYRFSNNTMVMTDSSSTVI
                     SLQNTVQTLEMYNNVIYGSKAGFQVYDVNEQIGPDTVHFGANNWVLAGTTSIPKAWIG
                     TLSGTDPGFV"
BASE COUNT         1076 a         2438 c         2283 g         1169 t
ORIGIN      
        1 ggcgccgggc cggccgcggc ctctccgatc gtcccgccgc tcgacgaccg ttcacctttg
       61 cgctggtagc tcatctcgcc attgtacgac agcggcccgc gaatccttct cgccagacgc
      121 cggcggcgcg aggtcggacc ctcgcaggtc ggcgggcggc aagagctgcc gcaaggaccg
      181 gctggttcat cgccggccgc gcggcgatcg gccccgcccg cggcggctga ccgtgcgccg
      241 cttcggagca ccatgccggc cggccctgct ggaatttgac caggcgcgtg gaaacgcgcc
      301 acgcatcgac gctgcactcc cgccggggca attctgcgag cttgcaatcg agagcgcgaa
      361 gcggaagata tcaacagggc gacgacgttg ctcgctcgag attgccagcc agcggcgatc
      421 gtcagctggc cgaaccttgc agctgcacgg tattgcgccg aacagggagg gagtccgaca
      481 tgtcgaatat ccaggtgacg ttcgactcga gtctgaacaa tgctcgcagt gagagcgcat
      541 tggttatcaa tcccattaat ccgctccaga tcgtggcggc gtcgaagaag ttcaacaata
      601 ttcacacata cgatttcacg cttgcaactg cctattcgat cgacggcggt cggagctggc
      661 aggactcggc gccatttaca ctgcctcccg gtgccaccgt catgaccgac ccgacgctgg
      721 cctgggacga tgccgggaac gtgttctccg tggggctggt gggcaataat ccgcctgagt
      781 gggatacgat cggaatcgcg atctacaagt cgaccgacgg cggccagacg tggggcagtc
      841 cgaacgtcat ccacacgagc acgggagacg acaagcagtg gatggccggt gacggcaacc
      901 aggcgagccc gtaccacggc agggtctatg cggtgtggga cgacggctcg gtgatgcggt
      961 tcgcgcgcac gctcgatcac ggcggcagct ggatcggcac gggcacaaac agcgtcgcca
     1021 gcacgagcct ggccaacgac tcgttcgcgc cggagatcaa cgtctctgcg aacggcgatg
     1081 tttacatcgt ctggatcgcc ggaagtacga tcaagatgct ggtctcgacc gacgggggcg
     1141 acagctttca cggtacgacg ccgccggcca ccggggtgac gacgttgagc tcgtccctgc
     1201 ccaacgtggg cgggttcccg gtgctccccg gcggcaactt ccgcgtcctc acgctcccga
     1261 ctgcctgcgt gtacggcaag accgtggcgg tcgcctggga tgacttccgg gagggcagct
     1321 cgcggatcta ccgtgcgctg tcgacggacg gcggcaccac ctggagcacg gggccgtcgg
     1381 gccagccgct gctcacgtcg gcgatcgatc cggccttgca ccacatcctt ccccagatcg
     1441 tcgccgaccc cgcgggcgtg ttcgggtgcg cgttctacga gttcggtccc aagcccacga
     1501 cgccgaagat cgacgtcatc atggcgcaat cgttcgacgg tggcgcgacg ttcgatcact
     1561 tcacggtgac cgatcagccg tgggatccgg tggtcgacgc gccgtggtcg catggcgatt
     1621 cgagcgtgac cttcatcggc gactacttcg ggctcgacgc cagcgccctg ggattctacc
     1681 cgctgtggac cgacacgcgg accggcattc aggagctgtt cacggcgatc gttcctgaaa
     1741 agaaatgtgt gttcgtggtg aaccgcagca cgctcggcca ggacgaggtc gacgcgcggc
     1801 gggccttgcc cggcggcgcg gtgatccccg acgcatttcg cgtcgtggtc gatggcttcg
     1861 cggcgaccga gctcggcatc accgggacgg ggtccacgct gccggtcgca tcgccggccg
     1921 ccggcatgac gatcgtcccg cgcggcaacg tctcggcgac gctcggctac gggcccgccg
     1981 tacagcggtt cacgttcttc tacgacatcg atttcggcgc gaccgacctg gcattcggct
     2041 tcccgggcgc gacgcggctg gtcacgctcc acgtcgacgc cggaccgacc agcgccgagg
     2101 cgcagctcga gctgatcaag cagcccgatc cattcctgct tcacggcgac acgttctggc
     2161 tcagcatcga cctgcgggtg ttcgtcgtgc gggccgggga gagcaagttc ggcgtgccgg
     2221 gcgtcgccga cgccggggat gcgccgcgct tcatccagca gctgatctcc acggtgacgc
     2281 cggcccagtt cgattcgctg tccgcggccg agggcgcttc gaagctctac ctccagccac
     2341 gcgacgagaa cggacagctg gtgttcaact tcgcactcgc gaaggtgcac tacatcgggc
     2401 tgatcggggc cacgaacgtg cgcgtgttct tccgcctgtt ccaggcccag accacgaccg
     2461 gcgtgttcga ctacccgccg ggcgcgcagt accggcgagc caccggcaat ccacacggcc
     2521 agccgatcgc cctgcccggc atcatcggca acgagtacgt cacgattccg tgcttcgccc
     2581 agccgcgcgt cgactccacc gtggtgtcgc tgaaccggca gaccgacgac cacaacgtcc
     2641 ggcccatcac tgctcatggc gatggctcgg aggtcgacac gttcttcggg tgctggctcg
     2701 acatcaacca gccgttcgca cccgacgggg tcacgcccaa caaccgcttg ccggtcgtcg
     2761 ttccgggcac ccatgtcgat ggcccgttca ccgatccgtc caatcctccg ctgccgatcc
     2821 agcaggcgat cttgcgcagc ctgcatcagt gcctgatcgc ggagatcgca ttcgatccgg
     2881 tcccgatccc ggtcggcaag gacccatcga actgggacaa gctcgcccag cgcaacctcg
     2941 cgtggagcga cgtcgggtcg tccgaggcgg tcagcacctt cgagatccga ccgacgccgc
     3001 tcgggcagcc tgcgagcttc ttaccggacg agctgatgat cgactggggc gatctcccga
     3061 gggggggcat cgcatcgatc catgtgccgg cagtatccgc cgacgagatc ctggcgatgg
     3121 ccggccggat gtacacgttg cacggcctgg tgcgcgcgga cgaccacacg ctgcaatgca
     3181 agacagccgg tgtcacgtac ctcccgatcc cgcccggcag cggttccgcc cacgccgggg
     3241 tgctgtcggt ggagctgcca gacgtcctga gacgggagca tgtctacaag ttcgtggtcc
     3301 ggcaggtgac caacgcgtac tctcgcggcg taccgccccg ccagatcgag tccgggcgcc
     3361 cggagaagga gggcgtcggg tcatcggcga cgcggaactt caagctgtgg cggcgcgtgc
     3421 tgggagcttt ccagctcagc attccggtca ggtcgaagca ggtgctgcgg gtcgtggagg
     3481 agcgcgactt cgccgtgctg cgctggatca accaggcgat tccgagcgcg aatcgctggc
     3541 accctgtctt ccggcgatac ctcgagatcg tgagccaccg ggtgcgggac ttcggcggca
     3601 accccctcca gatcccgccg tcgccgactg gggacggcgc gccaaggccc ccgcatcccg
     3661 gcgggcacgg cggcatcact ggcatggtcg agggactgat cttcgatcac cacggagagt
     3721 tcgaggggtt cattctcggc gatccgcccc acgcccgccg gttctacagt cgcgagcaga
     3781 agatcgagtt catcgccgaa cgggcctggc gtgagcggct gcggatcacg gtatgggcgg
     3841 agcaccacga gccccaccga cccgggacga tcgtcgttca cccgccgggg cacccgctgc
     3901 atcgctgatc ggctcgacga tattgctaca aaccgatctc gtattcgacg atctcgaggc
     3961 gctgcgcctc ggatcgccga gcgccacacg ctcaccagca actgagccac gccatcgtgg
     4021 cgccgcggac actgcgaaat cctggtagcg tgacgcgcac agcaggcgcg cggcgcgtcg
     4081 tggcgtcgta acacctcgac atcgcgtggt ggcgcatggc gtgcagtcat tcccggcgtg
     4141 gcagatccca tcgacgtccg cctgaccgcg ctcgaggcgc ggatcgcgca gctggaaggc
     4201 cacctgcagg tcgtgaccgt cgcgccgccg ccggccccgc ccggcgacgg tgccgcggcc
     4261 ctcgaggcaa ggctcggtgc gtactggctc agtcgcatcg ggatcgtgtc gttgatcagc
     4321 ggcgccgcgc ttgcgatcct cacctacttc gccgagctcg gatcggtcgt gcggatcgcg
     4381 ctcggctacg cggcggccgg cgcgatcgcg tggctcgggc tgcgcctcgc gcgcgcccac
     4441 ccgacgttcg gccgcatcct gttcggcggc ggtctcgccg tcgcctactt cgtgacgtac
     4501 gcgctgcact tcgtggccgc cctgcgcgtc atcgacagcg agccgctcgg catcgcgctc
     4561 gtggccgcgg cgatcgccgc catcgtcgcg atcgcgcacc gcatgcagtc cgagaccgtc
     4621 gccggcatcg cgctgttcct cggcttccac accggccttc tgtcggaggt caccgcgctg
     4681 tccttgatca ccaccacgct gctcgccgcc ggcgccgcgt tcttcctggt cgccaatcgc
     4741 tgggtgatcg tgccgctgtc cgcggtggcc gcggtctacg cgacccacgc aaccctcgcg
     4801 ttcggcaacg ccgcggtcgg cccggggctg cggatcgcgt tcgtcgcgat cgacttcgcg
     4861 ctgttcgcca ccgccctcct gatccggtcc gacaccgcca cgcgcccgcg ccgcctgctc
     4921 gcgctgctca actggctcgg cgcgctgctg ctcggcgggc acgccgcggc ggcgttctcg
     4981 cgcgatacct tgttcctcgg cctgtgcgga ttcgccggcg cgcagctcgc gatcgccgcg
     5041 atcgccaggc tgcgccgggc gccgggcagc gcagtcactc tcgagctcgg cctcgcgctc
     5101 gtcacgctcg cgctcgcgct cccggtccag ctgtccgccg gcccgctgct cgccggctgg
     5161 ctggcgctcg cgctcgccgc caccgcgatc gcgcgccgcg ccgcgccgtc gttcgccgtc
     5221 ctcgcgctgc tgctcgtgct cgccgggtac ggcgtggtcc aggtcagtca cctcggcgcc
     5281 ctcgcgcagc tcgcctgcgt gctcgcgatg atcgcggtcg agcgcggcca cgcgcccgcg
     5341 gctcgtgctg cgccgctgcg caccctcgcg atcgccggcg tcgcggccgg cctcctgcag
     5401 ctcgcccaga tcacggctcc gcccggctat cacacgctcg cgtgggtcgg cgccgggttc
     5461 gtgctgttcg cgctggggtt cgcgctgcgg acacgcgcct atcgctggtc gggcttcgcc
     5521 gcgctcgcgc tcgccgcgtc ccgcctgatc gccgtcgatg tgcgccggtt ctcgccggac
     5581 cagcggatcc tgacgttcgt gctcgccggc atcgcgttgc tcgtggtgtc gttcgtgtac
     5641 gcgcggcgcg aagcccgggg ctgatcgcga cgtgtcgacc gcgagcgcgc gcccgcgagg
     5701 tgctcggcga cgatgtgtcg tagcccgacc gaccaaggac ctgatcggcg acaacctcgt
     5761 acgctgccgc cgatcgaacg tgcgccgcct tgcgcttcag gcgtcgcgga gtcgcccgtt
     5821 tcgagcgcgc gcttgatgcc ccgggcaatc cgtgttgcga tccgccctcg atgaagatgc
     5881 gccttggtct gtgctggttg ggagtcggct cggtcctgtg ggccgctccc gcggtggccg
     5941 acacgctgag cgtgggcccg gggaagacgt atgcgggcat cgcggacgcc atgccgaacg
     6001 cgcatcccgg cgacgtgatc gaggtgcagg gaggcaacac ctatccgggg atgatctggc
     6061 tccgggacgg ctacggcgga acgaaggaca agccggtcac catccgaggg atcaaggtcg
     6121 gtggcaagcg gccggtgctg agcggcgtgg gctcaggcca gtacgagaac atcatcctcc
     6181 ttctcaactc caaccacgtc gtcatggaga gcttcgaggt catcggagac ggcggcgaca
     6241 ccaactactg tgtcgtacac aaggcagacg atgtcattct ccgtgacttc gtgatccaca
     6301 actgccttca ccagggcggg cacgtcggca acgacgacgg ctctggatcg ctcacactcg
     6361 agtacagcga gctctatcac aacggcaaag gtacggactc gcaccagatc tacatggcga
     6421 cggacgagac gatgtatcca ggctccgtct tcaggatcca gttctgctac gtacacgacg
     6481 ggctcggcgg caacaacgta cggtcgcgcg ccgagcgcaa cgagatctat tacaactggg
     6541 tcgaaggcgc gttctatcac gagctggatc tcgtcggtcc catcggcggt gattcgcttc
     6601 cgtttcgcga ggactcggac atcgtgggca acgtcctcat caagaaaaac tccgaatggc
     6661 gcatcgctcg cctcggcggc gacggagatg gcaatacggg agggcgctac cgcttctcca
     6721 ataacaccat ggtgatgaca gattcctcat cgaccgtcat cagcctccag aacacggtcc
     6781 agaccctcga gatgtacaat aacgtgatct acggctcgaa ggccggcttc caggtctacg
     6841 acgtcaacga gcagatcggc ccggacacgg ttcacttcgg ggcgaacaac tgggtcctcg
     6901 ccggcacgac cagcatcccc aaggcatgga tcggcacgtt gagtggcacc gacccaggct
     6961 tcgtcg
//
LOCUS       DASVOR010000274         2766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_90277,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000274 DASVOR010000000
VERSION     DASVOR010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90277"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            618..797
                     /locus_tag="VF516_05780"
     CDS             618..797
                     /locus_tag="VF516_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107217.1"
                     /translation="MLDPWIIEEILKKEEEQRRDSERGRVEVPVDRHRPDLGDRPAVT
                     PPREESERGVVIIDI"
     gene            972..>2766
                     /locus_tag="VF516_05785"
     CDS             972..>2766
                     /locus_tag="VF516_05785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX8107218.1"
                     /translation="MTRSGGHLFTIALGLAGWVVVLGLPALVQLPRTTATPMLWIALF
                     IVVILAARALAFRLVEGSVLSLDSAYYVAATLCVGAFGAGRIVAAALTLDAAARLYYA
                     RRRSGSDLAVSWTELGYVLYFGGMSGGLVALSGWIWHADALVGRHAETVEVAIRVVAI
                     DALLLAGHYALQGLRLRLLGRPWRAYLDELALPGIVAEASLMPIGVVLVLLYDPEQPL
                     GFVLLSLTYLLINLVFSRLSRTRKQLEERVSDLEILNATARRFAASLQLEELVEAVAR
                     ETCKAIPEAEAVALVHRRAGDRAGLVIDGFDRLTDRFLRRPMQEGEGAAGWVMTHGTV
                     RRIGDLSADPDIASRGGAHGIRSWLGVPLFMYGGCQGVIAVQSSHPRAFRADHQRLLE
                     SLALQIAAALQNAHLYELAMVDGLTGLFMRRYFDARVEEEIERSKRYGSSFSVVMVDV
                     DDFKHLNDTHGHLIGDRVLRSIANVVKSQMRGVDTAARYGGEELALILPRTEMVNAYN
                     VGERIRGAIAEMRVTTDGDPSRTLRVTASFGIASYPETKARDGEDLVRKADRALYRAK
                     KTGKNRVELYWSDESGPARPPTEDPVDTGRRE"
BASE COUNT          428 a          924 c          992 g          422 t
ORIGIN      
        1 ctcgaggcca acgtcgacga gccgatcacc gagcgcggca acgacgcgcg catgagctat
       61 ggtgatcgct ataccgtgca gttcctcaag gaggacggcg cctggaagct caaggatctc
      121 gactgaagcg tgtgtgcagg gggcgatccg atccgggccg ggggaatcgc aggccgaggg
      181 cgttgaaggc acgcacggag cgcctggatc gatcgaacca acgaacgtga acgagcaacc
      241 tgaaacctgg tcaacgcggt gggccgactg gaacgcgggg ggtccactcc ccgtggacac
      301 tcccggcgca ggtccgcaaa aaagtcagag cagcggcgat cgattcaacg gcgcgcccgg
      361 gctgaagaaa ccgcaacggc cgtttgcgtt atggggtgcg cagcacccgg cgcacgtccg
      421 gattctccgg cgatctcggc gggtacggat ttcgtacctg ggatgtggaa cgcccgaatt
      481 tcgatgcgtt agaataatta cgcgagaaac aacgtcccaa ggcccatggg ttgcaccatc
      541 tggtcacagg cgctacggat agtagagcgt gcgttcgagt agttagcccg cacatcagcc
      601 ccgaaaggcg gtccgctatg ttggatccct ggatcatcga agaaatcctc aagaaagaag
      661 aggagcaacg ccgcgatagc gagcgtgggc gggtggaggt gccggtcgat cgtcatcgtc
      721 cggatctcgg ggatcgtccg gccgtcacgc cgccccgcga ggagagcgag cgaggggtag
      781 tcatcatcga catctaacgc tgcaggcgcc ggcggcgccc caggacgccc cgggcgccgc
      841 gaggcggcct tccggcgggc gaagcggcgg cacacccctc ggcctcggca cttttgggac
      901 gtcatcgaac ggccctccta cacggagggc cgttcgcact ttcggcgagc gtagagtgat
      961 aggtagccgg tgtgacacgc tccgggggtc atctcttcac gatcgcgctc ggcctggccg
     1021 gctgggtggt cgtgctcggg cttcctgcgc tggtccagct acctcggacc accgcgacgc
     1081 ccatgctgtg gatcgcgctg ttcatcgtcg tgatcctggc ggcgcgcgcg ctggcgttcc
     1141 gcctggtcga gggcagcgtg ctgtcgctcg actcggcgta ctacgtcgcg gccacgctgt
     1201 gcgtcggggc gttcggcgcc ggacggatcg tcgccgcggc gctcacgctc gacgcggcgg
     1261 cccggctgta ctacgcccgc cggcgcagcg gcagcgacct cgcggtgagc tggaccgagc
     1321 tcggctacgt gctgtacttc ggcgggatga gcggcggcct ggtcgcgctg agcggctgga
     1381 tctggcatgc cgacgcgctg gtcggccggc acgccgagac cgtcgaggtc gcgatccgcg
     1441 tggtcgcgat cgacgcgctg ctgctcgccg ggcactacgc gctgcagggt ctgcggctgc
     1501 gcctgctcgg ccggccgtgg cgggcctacc tcgacgagct cgcgctgccc gggatcgtcg
     1561 ccgaggcgtc gctgatgccg atcggcgtgg tgctcgtgct gctctacgat cccgagcagc
     1621 cgctgggctt cgtgctgctg tcgctgacct acctgctgat caacctggtg ttctcgcggc
     1681 tgtcgcggac ccgcaagcag ctcgaggagc gggtgtccga cctcgagatc ctcaacgcca
     1741 ccgcccggcg gttcgccgcc tcgctccagc tcgaggagct ggtcgaggcg gtcgcgcgcg
     1801 agacctgcaa ggcgatcccc gaggccgagg ccgtggcgct ggtccaccgc cgcgccggcg
     1861 atcgcgccgg cctggtgatc gacggcttcg accggctgac cgatcgcttc ctccgccggc
     1921 cgatgcagga gggcgagggc gcggctggct gggtgatgac ccatggcacg gtccggcgga
     1981 tcggcgatct cagcgccgac cccgacatcg cgtcgcgcgg cggggcccac ggcatccggt
     2041 cgtggctcgg cgtcccgctg ttcatgtacg gcggctgcca gggcgtgatc gcggtgcaga
     2101 gcagccaccc gcgggcgttc cgcgccgacc accagcgcct gctcgagtcg ctggcgctgc
     2161 agatcgccgc ggcgctgcag aacgcccacc tctacgagct cgcgatggtc gacggcctca
     2221 ccgggctgtt catgcggcgc tacttcgacg cccgggtcga ggaggagatc gagcgctcca
     2281 agcgctacgg cagctcgttc tcggtggtga tggtcgacgt cgacgatttc aagcacctga
     2341 acgacaccca cgggcatctg atcggcgatc gcgtgctgcg gagcatcgcc aacgtcgtca
     2401 agtcacagat gcggggcgtc gacaccgcgg cgcgctacgg cggggaggag ctcgccctga
     2461 tcctgccgcg caccgagatg gtcaacgcct acaacgtcgg cgagcggatc cgcggcgcga
     2521 tcgccgagat gcgggtcacg accgacggcg atccgtcgcg cacgctgcgg gtcaccgcgt
     2581 cgttcggcat cgcgtcgtac cccgagacca aggcgcgcga cggcgaggac ctggtgcgca
     2641 aggcggatcg cgcgctgtac cgcgccaaga agaccggcaa gaaccgcgtc gagctgtact
     2701 ggagcgacga gtccgggccg gcccgcccgc cgaccgagga tccggtggac accggccggc
     2761 gcgagg
//
LOCUS       DASVOR010000275        10122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148504,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000275 DASVOR010000000
VERSION     DASVOR010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148504"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VF516_05790"
     CDS             complement(<1..820)
                     /locus_tag="VF516_05790"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8107219.1"
                     /translation="MTRRAVVLAAVVLALALFPLLQLAIGGLNYWLHMGLVVFMYVAM
                     ASSWNLLGGYAGYISLGHSAFYGIGGYVTGALMTFCGVSPLWSAPLAGVACFAIGLPV
                     GLITLRTRGPAFIISTIALLMLARLALDNTESLGGADGMSLPLPGLPADVQKLPFYYG
                     MLAVAVAAVVTSHRIRHAKLGLGLRAIAEDEVKAEAAGIPTALYKIVAFALSALFIAI
                     AGGIWGYYLTYLKPTIFFQILTSAQIVLMAILGGRGTVAGPVIGAIVLVAADELV"
     gene            complement(817..1692)
                     /locus_tag="VF516_05795"
     CDS             complement(817..1692)
                     /locus_tag="VF516_05795"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8107220.1"
                     /translation="MIDQLQVVILGATLGGVYALMASGLTLIFGVMRIVNIAHAAFMV
                     LAAYLSFWAFTLVHVDPIVSVAIDMPVLFGLGAAVYRGLFLRIADGRRYSEMTVLLTF
                     ALALVIEGVLGFLFTGVYRTTTPDYASDAFDLGPFYVPKGQLYATVLSLALLGVLLAF
                     LRLTRTGRAIRATMQNRAAAQLVGVNVRRISTLTFGIGTALAGASGALFSFLFPFFPS
                     KHWQLIASLLALIVLGGMGSLLGTFVGAMLLSIASAVVSDRFGPTWSPATFFLALFVI
                     LLVRPHGLFGKKAEL"
     gene            complement(1689..2456)
                     /locus_tag="VF516_05800"
     CDS             complement(1689..2456)
                     /locus_tag="VF516_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8107221.1"
                     /translation="MTVLEVHQVSAGYPGGPAILDGVSLAVERGQTCCVIGPNGAGKS
                     TLLKTICGLLRPRTGAIRFCGEVISGLRPDQILARGIAFVPQDRSLFPDMSVLENLQM
                     GGYTLRDRKKLAERIAAVFAMFPVLDQRRTQRARTLSGGQQQMLAMGRALLLDPAVIL
                     LDEPSIGLAPQIVQQVFDAVARLRAAGKTIVMVEQNARKGLECADRGCVLDLGQTRLE
                     GPAASLLADPRIEELYLGKRRRSAPGAPSRPAAEPPA"
     gene            complement(2453..3184)
                     /locus_tag="VF516_05805"
     CDS             complement(2453..3184)
                     /locus_tag="VF516_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8107222.1"
                     /translation="MTLLEVTDVTRRFGGVTAVDRCSFAIRAGSLTGLIGPNGAGKTT
                     MFNLIAGALRPDAGQIRLAGRRLDRLRPHQIARLGVSRTFQLTRELADLTVLENVVVH
                     APVRAIDLLRKSIGDAERARALSLLELVGLTRLADAPAGSLSYGQKKLLELAAALMPA
                     PRLVLLDEPAAGVNPALLDAIVGYIEHVHRTGVTFLIVEHNMDLVMSLCDPIIVMAHG
                     KVLAQGPRDEIQGDPRVLDAYLGAA"
     gene            complement(3197..4528)
                     /locus_tag="VF516_05810"
     CDS             complement(3197..4528)
                     /locus_tag="VF516_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8107223.1"
                     /translation="MNGKRLGGKLTRRAFVHGATGVAALSVFSGLWPGLGSGLARAQA
                     TKPIRIGVSLPLTGVFAIAGQKHRDGYQFWANLVNKDKKRQLLGRPVELVISDNRSDT
                     ETTVAQLERFISENQVDLLFGTFSSKLTFPASAAAEKAKMVYPVPAGGALKIWERGFQ
                     YIFYFQQNAAETIGQAPIDVLAHARDGKRIKPAEFPASAGIVNADDFFSNAIAAGLAG
                     DGTLAPGVLKTAGIHLAFRQKWPEGFTDWITLANSLKGKKPDLVMACTASPDEAIQLV
                     RALRTVRFNPPGLYVSQGSQAEFKQAVGDAANGILTHAAWHAAAPYQGLLAGEKYSNQ
                     QFQKDFQAEFKREADEDEAIPFAVCQGMEQAVRAVGSTDNQKLRAWLAARTKADPVRT
                     VLGDFYWDKRGLPVNRPFLVLQWQNNKLEFVWPQGEFAGMKDVQWPKPKWG"
     gene            4926..6257
                     /locus_tag="VF516_05815"
     CDS             4926..6257
                     /locus_tag="VF516_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX8107224.1"
                     /translation="MAHELREPSFTPSVITRDKVLAPEALKPGYNWNRPISAPGHSAV
                     DFEQRVDFRRLHDYRLARARQALAGSGLGAVLCFDNNNIRYLTSTVIGEWSRDKMCRF
                     ALLTGNGEPYLWDFGSAAVHHRLYAPWLPHDHCLAGMVGLRGSVRPEVGLFKRAAEEI
                     KAILVEQGVAGMPIGLDITELPMLFALQDAGLEVRDGQQTMLDARQIKNVDEITLLNM
                     SAAMVDGVYQVIAEHLKPGVRECDIVALANKTLYEMGSDDVEGINAIAGERCSPHPHN
                     FTDRIIRPGDQAFFDIIQSFTGYRTCYYRTFNVGRATDAQRDAYKKAREWMDAAIALV
                     KPGVSTDRIAAVWPRAEEFGFASEMEAFALQFGHGLGLALHERPIISRLVSFDSPMEI
                     QEGMVFALETYCPARDGHSAARIEEEVVVTRDGCRVITLFPAEELFIANRY"
     gene            6271..7269
                     /locus_tag="VF516_05820"
     CDS             6271..7269
                     /locus_tag="VF516_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX8107225.1"
                     /translation="MTSSPDGIDGATRFALYRSLLEIRVVEKRAYDLFLQNLVKGTSH
                     LALGQEAVAAGFAVAMRPDDYTFCTYRGHHHTLARGAPMVGVLGELMGRKCGLMAGKG
                     GSMHLTSVRHGVMGSYAIVGAHLPIAAGAAWSAQYRGTEQVAVCFFGDGTTNIGAFHE
                     ALNLAVVWKLPVIFVCENNLYMEYTPIASVTAVEHPAADRAAAYGLPRVIVDGNDADA
                     VYRLARETIAAARAGRGPALIEAKTYRHSGHSRADPAKYRPPGELEDWLRRDPVTLYR
                     TRLLEAGVPEAALREIEVATQGAVDAATEAAKASPPAPIEVVEQDVWSDGGSAWRN"
     gene            7257..8234
                     /locus_tag="VF516_05825"
     CDS             7257..8234
                     /locus_tag="VF516_05825"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8107226.1"
                     /translation="MAELTYREAVAAGIAQEMARDERVVMLGEDIAGAGGVFKTTVGL
                     LERFGPKRVRDTPISEQAIVGAAMGAAMTGLVPIAEIMFSDFFAVCWDMIANEIAKAR
                     YMTAGQMALPLVIRTANGSGSRFGAQHSQSVENWAMAVPGLKVVAPATPADVKGLMAA
                     AVRDPDPVIFFEHKGLFATKGEVPDGEHVDRLGTAKVLRQGSDITLVALAAMVPRALE
                     AAERLDREHQISATVVDVRSLVPLDTQTILAEVTRTGRLYTVEENPRLCGWGAELVSI
                     AAEERFYDLDGPIIRITTPHVPLPAADALEDHAVPSVQRIIDTVVRTAG"
     gene            8246..9514
                     /locus_tag="VF516_05830"
     CDS             8246..9514
                     /locus_tag="VF516_05830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX8107227.1"
                     /translation="MPQLGETVREGTISAWRKQVGDPVSPDEPLFEVETDKVTVDIPA
                     PAAGVLVEIVVPVGERVEVGARLAVIRVAGEAMFSMTASAKVVTLSSARPTVPAMPVA
                     IAAVSPDPEAAGASGRPTMPAIPTAIAIRAAGAELRLSPVVKRLLAEHQIDDPSVIPG
                     TGADGRITRDDVLAYVASRGGAPADTLVPMSRIRQRTAEHMVRSVATSPHVLQAVEVD
                     FHAVERLRGAAGEAWRAREGFSLSYLPFVARAVCQAIPEFPHVNASVRGDALVVHHRV
                     HLGIAVDLDRQGLVVPVVKDAGGKTVRALAVEIHRLADRARRGQLVPDDLTEGTYTIS
                     NSGPFGTLITAPIIHQPQVAILSTDGVRKRPVVIERPEAQGGDAIEIRPVGVLAQCFD
                     HRAFDGAYSAAFLRRVREILEQANFEAELT"
     gene            9541..>10122
                     /locus_tag="VF516_05835"
     CDS             9541..>10122
                     /locus_tag="VF516_05835"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8107228.1"
                     /translation="MTSLTQAQWKQRKVGWIGAGRMGMAMATRLAKAGCDLTIHNRTR
                     AKAEPLAQHGAKIADRLQDLAGCDLVFAIVSTGKDLESVLVGPEGLFAGGRGPRIVVD
                     CTTIAIEESAVLREALAARGAQFLAAPVSGNAKVARAGKLSFVASGPRAVFDEVEPYL
                     AALGQGASYVGEGELSRMVKICHNVFLGVVIQSL"
BASE COUNT         1512 a         3527 c         3687 g         1396 t
ORIGIN      
        1 cgaccagctc gtcggcggcg accagcacga tcgcgccgat caccgggccg gcgaccgtgc
       61 cgcggccgcc gaggatcgcc atcaggacga tctgcgccga ggtcaggatc tggaagaaga
      121 tggtgggctt gaggtacgtg aggtagtagc cccagatccc gcccgcgatc gcgatgaaca
      181 gcgcgctcag cgcgaacgcg acgatcttgt acagcgcggt cgggatgccg gcggcctcgg
      241 ccttgacctc gtcctcggcg atcgcgcgca gccccaggcc gagcttggcg tgccggatcc
      301 ggtgcgacgt gaccaccgcg gcgaccgcga ccgcgagcat gccgtagtag aacggcagct
      361 tctggacgtc ggcggggagc ccgggcagcg gcaggctcat gccgtcggcg ccgcccagcg
      421 actcggtgtt gtcgagcgcg agccgggcga gcatgagcag cgcgatcgtc gagatgatga
      481 acgccgggcc gcgggtccgc agcgtgatca ggccgaccgg cagcccgatc gcgaagcacg
      541 cgacgccggc gagcggcgcc gaccacagcg gcgacacgcc gcagaacgtc atcagcgcgc
      601 cggtgacgta gccgccgatg ccgtagaacg cgctgtggcc gagcgagatg tacccggcgt
      661 agccaccgag caggttccag ctcgacgcca tcgcgacgta catgaacacg acgaggccca
      721 tgtgcagcca gtagttgagg ccgccgatcg cgagctggag cagcgggaac agcgcgagcg
      781 cgagcacgac cgccgcgagc accaccgcgc gccgggtcac agctccgcct tcttgccgaa
      841 caggccgtgc ggccgcacca gcaggatcac gaacagcgcc aggaagaacg tcgccggcga
      901 ccaggtcggc ccgaaccggt cgctgaccac cgccgaggcg atcgacagca gcatggcgcc
      961 gacgaaggtc ccgagcaggc tgcccatgcc gccgagcacg atcagcgcga gcaggctggc
     1021 gatcagctgc cagtgcttgg acggaaagaa cgggaacaaa aagctgaaca gcgcgcccga
     1081 cgcgccggcc agcgcggtgc cgatgccgaa cgtcagcgtc gagatccgcc ggacgttgac
     1141 gccgaccagc tgcgcggcgg cgcggttctg catggtcgcg cggatcgccc ggccggtgcg
     1201 ggtcagccgc aggaacgcga gtaatacgcc aagcagggcg aggctgagca cggtggcgta
     1261 gagctggccc ttggggacgt agaacggccc gaggtcgaac gcatcgctgg cgtagtcggg
     1321 cgtcgtcgtg cggtacacgc cggtgaacag gaagccgagg acgccctcga tgaccagcgc
     1381 gagcgcgaag gtcaggagca cggtcatctc gctgtaccgg cggccgtcgg cgatccgcag
     1441 gaacagcccg cggtacaccg ccgcgcccag cccgaacagg accggcatgt cgatcgcgac
     1501 cgagacgatc ggatcgacgt ggaccagcgt gaacgcccag aacgacaggt acgccgcgag
     1561 caccatgaac gcggcgtgcg cgatgttgac gatccgcatc acgccgaaga tcagggtcag
     1621 ccccgacgcc atcagggcgt acacgccgcc cagcgtggcg ccgaggatca cgacctggag
     1681 ctgatcgatc acgcgggcgg ctccgccgcg ggccgcgaag gggcgccggg cgcgctgcgg
     1741 cgccgcttgc ccaggtagag ctcctcgatc cgagggtcgg ccaggaggct cgccgccggg
     1801 ccttcgagcc gggtctggcc gaggtcgagc acgcagccgc ggtcggcgca ctccaggccc
     1861 ttgcgcgcgt tctgctcgac catgacgatg gtcttgccgg ccgcgcgcag ccgggccacg
     1921 gcgtcgaaca cctgctggac gatctgcggc gccagcccga tcgacggctc gtcgagcagg
     1981 atcaccgcgg ggtcgagcag cagcgcgcgg cccatcgcca gcatctgctg ctggccgccg
     2041 ctcagcgtgc gggcgcgctg cgtccggcgc tggtcgagca ccgggaacat cgcgaacacc
     2101 gcggcgatcc gctcggcgag cttcttgcgg tcgcgcagcg tgtagccgcc catctggagg
     2161 ttctcgagca cggacatgtc ggggaacagg ctgcgatcct ggggcacgaa cgcgatgccg
     2221 cgggcgagga tctggtcggg gcgcagcccg ctgatcacct cgccgcaaaa ccggatcgcg
     2281 ccggtgcgcg gccggagcag gccgcagatc gtcttgagca gggtcgactt gccggcgccg
     2341 ttggggccga tgacgcagca ggtctggccg cgctcgaccg cgaggctgac gccgtcgagg
     2401 atggcgggtc cgccggggta gcccgccgac acctggtgga cctcgagcac cgtcacgcgg
     2461 cccccaggta ggcgtccagc acgcgggggt cgccctggat ctcgtcgcgc gggccctgcg
     2521 ccagcacctt gccgtgcgcc atgacgatga tcgggtcgca caggctcatc accaggtcca
     2581 tgttgtgctc gacgatcagg aacgtcacgc cggtgcgatg gacgtgctcg atgtagccga
     2641 cgatcgcgtc gagcagggcg gggttgacgc cggcggccgg ctcgtcgagc aggaccagcc
     2701 tgggcgcggg catcagcgcg gccgcgagct cgagcagctt cttctggccg tacgacaggc
     2761 tgccggccgg cgcgtcggcc agccgggtca ggccgaccag ctccaggagg ctcagcgcgc
     2821 gggcgcgctc ggcgtcgccg atgctcttgc gcagcagatc gatcgcgcgg accggcgcgt
     2881 ggaccacgac gttctcgagc acggtcagat cggcgagctc gcgggtgagc tggaacgtcc
     2941 ggctcacgcc gagccgggcg atctggtgcg ggcggagccg atcaagccgc cggccggcca
     3001 gccggatctg gccggcgtcg ggccggagcg ccccggcgat caggttgaac atcgtggtct
     3061 tgccggcgcc gttggggccg atcagcccgg tcaggctgcc ggcgcggatc gcgaacgagc
     3121 agcgatcgac cgcggtgacc ccgccgaacc gccgcgtgac gtctgtgacc tccagcagcg
     3181 tcatgccggg cgcgcctcac ccccatttcg gcttgggcca ctgcacatct ttcatcccgg
     3241 cgaactcgcc ctggggccag acgaactcca gcttgttgtt ctgccactgc aggacgagga
     3301 agggccggtt caccggcagt ccgcgcttgt cccagtagaa gtcgccgagc acggtgcgca
     3361 ccggatcggc cttggtccgc gcggcgagcc acgcgcgcag cttctggttg tcggtcgagc
     3421 cgaccgcgcg caccgcctgc tccatgccct ggcagaccgc gaacgggatc gcctcgtcct
     3481 cgtcggcctc gcgcttgaat tcggcctgga agtccttctg gaactgctga ttgctgtact
     3541 tctcgccggc gagcaggccc tggtacgggg ccgcggcgtg ccacgcggcg tgggtcagga
     3601 tgccgttggc cgcgtcgccg accgcctgct tgaactcggc ctgcgagccc tgcgagacgt
     3661 acagaccggg cggattgaac cggacggtgc gcagcgcgcg gaccagctgg atcgcctcgt
     3721 cgggcgacgc cgtgcacgcc atcacgaggt cgggcttctt gcccttgagc gagttggcga
     3781 gcgtgatcca gtcggtgaag ccctcgggcc acttctggcg gaacgcgagg tggatcccgg
     3841 cggtcttgag cacgccgggc gcgagcgtgc cgtcgccggc gagcccggcc gcgatcgcgt
     3901 tggagaagaa gtcatcggcg ttcacgatcc cggccgacgc cgggaactcc gccggcttga
     3961 tccgcttgcc gtcgcgcgcg tgggcgagca cgtcgatcgg cgcctggccg atggtctcgg
     4021 ccgcgttctg ctggaagtag aagatgtact ggaagccgcg ctcccagatc ttgagcgcac
     4081 cgccggccgg caccgggtag accatcttcg ccttctcggc cgccgccgag gccgggaacg
     4141 tgagcttgct cgagaacgtg ccgaacagca gatcgacctg gttctcgctg atgaaccgct
     4201 cgagctgcgc gaccgtggtc tcggtgtcgg accggttgtc actgatcacc agctcgacag
     4261 gccggccgag cagctggcgc ttcttgtcct tgttgaccag gttggcccag aactggtagc
     4321 cgtcgcggtg cttctggcct gcgatcgcga acacaccggt gagcggcagc gatacgccga
     4381 tccggatcgg cttggtcgcc tgggccctgg cgaggccgga gccgaggccg ggccagaggc
     4441 cggaaaagac actcagcgcc gcaacgccgg ttgcgccgtg gacgaatgcc ctccttgtaa
     4501 gcttaccgcc gagccgcttg ccgttcatcg gggagacctc cgtggctggt agtccgcgcc
     4561 ggcccgcgat accgtgctgc aggcgctgca tcattgtccc gggagtgcag gcaagtcaac
     4621 cgccgaacac ctccggtcgc ggggcaatct ggcgtgtcag atatccattg tccaggttgc
     4681 gcgcagcggc atggcgagtc gctgcaaccg ccgggtggtc gaagggtgcg cagctgccca
     4741 ctcgcgtcgc gccgcccgcc gcgccgcccg gtgctccggc ggtgccgggc tttgactcgc
     4801 gcgcggggtc gcggtaggtt caccgcgggt tctcgtctgt ccagcatcgt ctgccgcggg
     4861 cattgaccgg caccgggccg ggcggtgcgc gatcctggtc ggcgcgggcc gaaaggtttt
     4921 ccagcatggc ccacgaactg cgcgagccgt ctttcacgcc ctcggtgatc acccgcgaca
     4981 aggtgcttgc gcccgaggcg ctcaagcccg gctacaactg gaaccgcccg atctccgctc
     5041 ccgggcactc ggcggtcgac ttcgagcagc gggtcgactt ccgccgcctg cacgactacc
     5101 ggctcgcccg cgctcgccag gcgctcgccg gctcggggct cggcgcggtg ctgtgcttcg
     5161 acaacaacaa catccgctac ctgaccagca cggtgatcgg cgagtggtcg cgcgacaaga
     5221 tgtgccggtt cgcgctgctg accggcaacg gcgagccgta cctgtgggac ttcggctcgg
     5281 cggcggtcca ccaccggctg tacgcgccgt ggctgcccca cgatcactgc ctcgcgggca
     5341 tggtcgggct gcgcggctcg gtgcggcccg aggtcgggct gttcaagcgc gccgccgagg
     5401 agatcaaggc gatcctggtc gagcagggcg tcgccgggat gccgatcggc ctcgacatca
     5461 ccgagctgcc gatgctgttc gcgctgcagg acgccgggct cgaggtccgc gacggccagc
     5521 agaccatgct cgacgcccgc cagatcaaga acgtcgacga gatcaccctg ctcaacatgt
     5581 cggcggcgat ggtcgacggc gtctaccagg tgatcgccga gcacctgaag cccggcgtcc
     5641 gcgagtgcga catcgtggcg ctcgccaaca agacgctgta cgagatgggc tcggacgacg
     5701 tcgagggcat caacgcgatc gccggcgagc gctgcagccc gcacccccac aacttcaccg
     5761 accggatcat ccggccgggc gaccaggcgt tcttcgacat catccagtcg ttcaccgggt
     5821 accgcacctg ctactaccgg acgttcaacg tcgggcgcgc caccgacgcc cagcgcgacg
     5881 cctacaagaa ggcgcgcgag tggatggacg cggcgatcgc gctggtcaag ccgggcgtct
     5941 ccaccgaccg gatcgcggcg gtatggccca gggccgagga gttcgggttc gcgagcgaga
     6001 tggaggcgtt cgcgctccag ttcggccacg gcctgggcct ggcgctccac gagcggccga
     6061 tcatcagccg cctggtgtcg ttcgacagcc cgatggagat ccaggagggc atggtgttcg
     6121 cgctcgagac ctactgcccg gcgcgcgacg gccactccgc ggcgcggatc gaggaggagg
     6181 tcgtggtgac ccgggacggc tgccgggtga tcacgctgtt ccccgccgag gaactgttca
     6241 tcgccaatcg ctactgagga tggaggccct gtgacgagca gcccggacgg catcgacggc
     6301 gccacgcggt tcgcgctcta tcgctcgctg ctcgagatcc gcgtggtcga gaagcgcgcc
     6361 tacgacctgt tcctccagaa cctggtcaag ggcaccagcc acctcgcgct cggccaggag
     6421 gcggtcgcgg ccgggttcgc ggtggcgatg cggccggacg actacacgtt ctgcacctac
     6481 cgcggccacc accacaccct ggcgcgcggc gcgccgatgg tcggcgtgct cggcgagctg
     6541 atggggcgca agtgcggcct gatggccggc aagggcggct cgatgcacct gaccagcgtc
     6601 cgccacggcg tgatgggctc gtacgcgatc gtcggcgcgc acctgccgat cgcggccggc
     6661 gcggcgtggt cggcgcagta ccgcggcacg gagcaggtcg cggtgtgctt cttcggcgac
     6721 ggcaccacca acatcggcgc gttccacgag gcgctcaacc tcgccgtggt gtggaagctg
     6781 cccgtgatct tcgtgtgcga gaacaacctc tacatggagt acacgccgat cgccagcgtc
     6841 accgcggtcg agcacccggc ggccgaccgc gcggcggcgt acggcctgcc gcgggtgatc
     6901 gtcgacggta acgacgcgga cgcggtgtac cggctggcgc gcgagaccat cgccgcggcg
     6961 cgcgccggcc gcggccccgc gctgatcgag gccaagacct atcgccacag cggccactcg
     7021 cgggccgacc cggcgaagta ccggccgccc ggcgagctcg aggactggct gcgccgcgat
     7081 ccggtgacgc tgtatcgcac ccggctgctc gaggccgggg tccccgaggc cgcgctgcgc
     7141 gagatcgagg tcgcgaccca gggcgcggtc gacgccgcga ccgaggccgc caaggcctcg
     7201 ccgccggcgc cgatcgaggt cgtcgagcag gacgtgtggt ccgacggagg ttcggcatgg
     7261 cggaactgac ctatcgcgag gcggtcgcgg ccggcatcgc gcaggagatg gcgcgcgacg
     7321 agcgcgtggt gatgctcggc gaggacatcg cgggggcggg cggcgtgttc aagaccacgg
     7381 tcgggctgct cgagcggttc ggccccaagc gggtccgcga cacgccgatc tccgagcagg
     7441 cgatcgtcgg cgcggcgatg ggcgcggcga tgaccggcct cgtcccgatc gccgagatca
     7501 tgttctcgga cttcttcgcc gtgtgctggg acatgatcgc caacgagatc gccaaggcgc
     7561 gctacatgac cgcgggccag atggcgctgc cgctggtgat ccggaccgcc aacggctcgg
     7621 gctcgcggtt cggcgcccag cactcgcaga gcgtcgagaa ctgggcgatg gcggtgcccg
     7681 ggctcaaggt cgtggcgccg gcgacgccgg ccgacgtcaa gggcctgatg gcggccgcgg
     7741 tgcgcgatcc ggatccggtg atcttcttcg agcacaaggg gctgttcgcg accaagggcg
     7801 aggtgccgga cggcgagcac gtcgaccggc tcggcaccgc caaggtgctg cgccagggca
     7861 gcgacatcac gctggtcgcg ctggcggcca tggtgccgcg cgcgctcgag gccgccgagc
     7921 ggctcgaccg cgagcaccag atctcggcga ccgtggtcga cgtgcggtcg ctggtgccgc
     7981 tcgacaccca gaccatcctc gccgaggtga cccggaccgg ccggctgtac accgtcgagg
     8041 agaacccgcg gctgtgcggc tggggcgccg agctggtgtc gatcgccgcc gaggagcggt
     8101 tctacgacct cgacggcccg atcatccgga tcaccacgcc gcacgtgccg ctgccggcgg
     8161 ccgacgcgct ggaggaccac gcggttccct ccgtgcagcg gatcatcgac accgtggtga
     8221 ggacggcggg ctgatggacg tcgtcatgcc gcagctgggc gagaccgtgc gcgagggcac
     8281 gatctcggcg tggcgcaagc aggtcggcga cccggtcagc cccgacgagc cgctgttcga
     8341 ggtcgagacc gacaaggtca cggtcgacat cccggcgccc gccgccggcg tgctcgtcga
     8401 gatcgtcgtg ccggtcggcg agcgggtcga ggtcggggcg cgtctggcgg tgatccgggt
     8461 cgccggcgag gcgatgttct cgatgaccgc gagcgcgaag gtcgtgacgc tgtccagcgc
     8521 ccggcccacg gtgcccgcga tgccggtggc gatcgcggcg gtgagcccgg accccgaggc
     8581 cgcgggcgcg tccgggcggc ccacgatgcc cgcgatcccg accgcgatcg cgatccgggc
     8641 cgcgggtgcc gagctccggt tgtcgccggt ggtcaagcgg ctgctcgccg agcaccagat
     8701 cgacgatccg tcggtgatcc cgggcaccgg cgccgacggc cggatcaccc gcgacgacgt
     8761 cctggcctac gtcgcgagcc gcggcggcgc gccggccgac accctcgttc cgatgagccg
     8821 gatccgccag cgcaccgccg agcacatggt tcgctcggtg gcgaccagcc cgcacgtgct
     8881 gcaggcggtc gaggtcgact tccacgcggt cgagcggctg cgcggcgccg ccggcgaggc
     8941 gtggcgggcg cgcgaggggt tctcgctcag ctacctgccg ttcgtcgcgc gcgcggtgtg
     9001 ccaggcgatc cccgagttcc cgcacgtcaa cgcgagcgtg cgcggcgacg cgctggtcgt
     9061 gcaccaccgc gtgcacctcg ggatcgcggt ggatctcgat cgccagggcc tggtcgtgcc
     9121 ggtggtgaag gacgccggcg gcaagaccgt ccgcgcgctc gccgtcgaga tccaccggct
     9181 cgccgaccgc gcgcggcgcg gccagctcgt gcccgacgac ctgaccgagg gcacctacac
     9241 gatctcgaac tccggcccgt tcggcacgct gatcaccgcg ccgatcatcc accagccgca
     9301 ggtcgcgatc ctgtcgaccg acggggtgcg caagcggccg gtggtgatcg agcgtcccga
     9361 ggcacagggc ggcgacgcga tcgagatccg gccggtcggc gtgctcgccc agtgcttcga
     9421 tcaccgcgcg ttcgacggcg cgtactcggc ggcgttcctg cgccgcgtgc gcgagatcct
     9481 ggagcaagcc aacttcgagg ccgagctgac ctgacagctc gcagacaaca aggagtgccc
     9541 atgacgtccc tgacacaggc ccagtggaag cagcgcaagg tggggtggat cggcgcgggg
     9601 cggatgggca tggcgatggc gacccgcctg gcgaaggccg gctgcgacct caccatccac
     9661 aaccggaccc gcgccaaggc cgagccgctc gcgcagcacg gcgcgaagat cgccgaccgg
     9721 ctgcaggatc tcgccggctg cgatctcgtg ttcgccatcg tgtcgaccgg caaggacctc
     9781 gagagcgtgc tggtcggccc cgaggggctg tttgcgggcg ggcgcggccc gcggatcgtc
     9841 gtcgactgca ccacgatcgc gatcgaggag tcggcggtgc tgcgcgaggc gctcgccgcc
     9901 cgcggcgcgc agttcctcgc cgcgccggtc agcggcaacg ccaaggtcgc gcgcgccggc
     9961 aagctcagct tcgtcgcctc cgggccgcgg gccgtgttcg acgaggtcga gccgtacctc
    10021 gccgcgctcg gccagggcgc gagctacgtc ggcgagggcg agctgtcgcg catggtcaag
    10081 atctgccaca acgtgttcct cggcgtggtg atccagtcgc tc
//
LOCUS       DASVOR010000276         3050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_119384,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000276 DASVOR010000000
VERSION     DASVOR010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3050
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119384"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF516_05840"
     CDS             <1..197
                     /locus_tag="VF516_05840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107229.1"
                     /translation="ARDATAAEHAWREAGRLWERAADRETDARRRAAYTANADRARKA
                     ADEPQAADPASDDSLSSPTN"
     gene            404..1582
                     /locus_tag="VF516_05845"
     CDS             404..1582
                     /locus_tag="VF516_05845"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEX8107230.1"
                     /translation="MTASPAPRVRIESVDLTRGIIMIVMALDHVRDFLAVPANPTNLA
                     TTTPALFFTRWITHVCAPVFCLLTGVGARLSLGRKSRAELARFLWTRGLWLIALDTVV
                     ARCLVYQFNVDFQLTVLDVLWSLGWSMIALSALIYLPTAAIAVLGAALVAGHNLLDGV
                     RPASFGALAPVWNILHAPGPIGAGTAHSVFVAYPLIPWVGVMALGWCLGGIYAWPVER
                     RRRTLSWLGLGLTGAFVVLRAMNGYGDPAPWAPRPTLAVTVLSFLNTTKYPPSLAFLL
                     MTLGPALILLRVAEAPARWLEPVRVIGKVPLFYFLVHFAVIHAIAVVISAVSFGTVGG
                     MFDSPTIDRYPVTFPPGWGYSLPGVYAAWIGVVIAVYPLCRWYAAVKQRRRDWWLGYL
                     "
     gene            complement(1692..>3050)
                     /locus_tag="VF516_05850"
     CDS             complement(1692..>3050)
                     /locus_tag="VF516_05850"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF006614.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX8107231.1"
                     /translation="DVLRNIMLRDPHHVYGRVYVVSAYAGVTNQLLEHKKTGEPGIYA
                     RFAAGTDYAAALDALTARLVAINHELAPVGLPLDVANGFIERRMATLRGYLDSMRHVM
                     ASGYVHRDSLLLAAREMLAAIGEAHSAFNSVEILKAKGVRAYLMDLTGFHDDDPLTID
                     DRIRASFQAIDPREQVVVATGYTKGVEGIMREFDRGYSEVTFSKIAVELKVAEAVIHK
                     EFHLSSADPEIVGAGNTVVVGNTNYDVADQLADVGMEAIHPKAAKPMELSGIPIRLKN
                     TFEPEHPGTLITKDFIGKEARIEVIAGSAKVVIVEIHDPLMVGTVGFDLGVMQILANH
                     RISYIVKATNANSISHILWEKAATPALIEELQARYDKVTMVRAAVVCVIGSNLAIPGV
                     LARAAQVLADNQINIICVSQSVRQSNMQFVIDRGDYKKAIIALNQALCLDGSQRNGSN
                     TA"
BASE COUNT          399 a         1027 c         1091 g          533 t
ORIGIN      
        1 cggcgcgcga tgcgaccgcg gccgagcacg cgtggcgcga ggccgggagg ctgtgggagc
       61 gcgccgccga ccgcgagacc gacgccaggc gccgggccgc atacacggcc aatgccgacc
      121 gcgcccgcaa ggccgccgac gagccgcagg ccgccgaccc ggccagcgac gattcgctgt
      181 ccagcccgac caactgaccg ggtgagcgcg tggtcgggct ctctcgaccc ggcgccgcgc
      241 tcgcgacgag cgccacggcg catcgggatg ggcaagcacg gcacaccctg gcgcaggaac
      301 atccgatctc ggtaagctac ggcatgctcg gggcgccgat cgactccggg gcgatcgacc
      361 aggtcgctgc gcccgcggat atcccgggct ggccgaggtt cgcatgacgg cgtcgccagc
      421 cccacgcgtt cggatcgaat cggtcgacct cacgcgcggc atcatcatga tcgtgatggc
      481 gctcgatcac gtgcgtgact tcctggcggt gccggcgaac ccgaccaacc tggcgaccac
      541 cacgccggcg ctgttcttca cacgctggat cacccacgtt tgcgcgccgg tgttctgcct
      601 gctcaccggc gtcggtgcgc ggctgtcgct cggccgcaag tcgcgggccg agctggcgcg
      661 ctttctgtgg acgcgcgggc tgtggctgat cgccctcgac accgtggtgg cccgctgcct
      721 ggtgtaccag ttcaatgtcg acttccagct caccgtgctc gatgtcctgt ggtcgctcgg
      781 ctggtcgatg atcgcgctgt cggcgctgat ctacctgccg acggcggcga tcgcggtgct
      841 cggcgccgcg ctggtcgccg gccacaacct gctcgacggc gtccgcccgg ccagcttcgg
      901 tgcgctggct ccggtctgga acatcctgca cgcgccgggg ccgatcggcg ccggaaccgc
      961 gcacagcgtg ttcgtggcct atccgctgat cccgtgggtc ggcgtgatgg cgctcggctg
     1021 gtgcctcggc gggatctacg cctggcccgt cgagcgccgc cgccgcacgc tgtcgtggct
     1081 cggcctcggg ctgaccggcg cgttcgtggt gctgcgcgcc atgaacggct acggcgatcc
     1141 cgcgccctgg gcgccgcggc ccacgctcgc ggtcaccgtg ctgtcgttcc tcaacaccac
     1201 gaagtatccg ccgtcgctgg cgtttctgct gatgacgctc ggcccggcgc tgatcttgct
     1261 gcgcgtcgcc gaggctccgg cgcgctggct cgagccggtc cgggtcatcg gcaaggtgcc
     1321 gctgttctac ttcctcgttc acttcgcggt gatccacgcg atcgcggtcg tgatcagcgc
     1381 ggtctcgttc ggcacggtgg gtgggatgtt cgactcgccg acgatcgatc gctacccggt
     1441 cacctttcca ccgggctggg gctactcgct gcccggggtg tacgcggcgt ggatcggcgt
     1501 cgtgatcgcg gtctacccgc tgtgccggtg gtacgcggcg gtgaagcagc gccggcgcga
     1561 ctggtggctc ggctacctgt gactgcgccg gcggcgcctc gtacgcgaac accttctcca
     1621 ggtggacggc agcgcccgct ggacatccag ggccagctcc acgcggctcc gacgggcgct
     1681 gtggtcaccg atcacgcggt gttcgagccg ttgcgctgcg agccatcgag gcagagcgcc
     1741 tggttgagcg cgatgatcgc cttcttgtag tcgccgcggt cgatgacgaa ctgcatgttg
     1801 ctctggcgca ccgattgcga gacacagatg atgttgatct ggttgtcggc gaggacctgg
     1861 gcggcgcggg cgaggacgcc ggggatggcg aggttggagc cgatgacgca gaccaccgcg
     1921 gcgcggacca tggtgacctt gtcgtagcgc gcctgcagct cctcgatcag cgccggggtg
     1981 gcggccttct cccagaggat gtggctgatc gagttcgcgt tggtggcctt gacgatgtag
     2041 gagatgcgat ggttggccag gatctgcatg acgccgaggt cgaagccgac ggtgccaacc
     2101 atgagcgggt cgtggatctc gacgatgacg accttggccg agccggcgat gacctcgatg
     2161 cgcgcttcct tgccgatgaa gtccttggtg atcaaggtgc cggggtgctc gggctcgaag
     2221 gtgttcttga gccggatcgg gatgcccgac agctccatcg gcttggccgc cttgggatgg
     2281 atcgcttcca tgccgacgtc ggcgagctgg tcggcgacgt cgtagttggt gttgccgacg
     2341 acgacggtgt tgccggcccc gacgatctcg gggtcggccg acgacaggtg gaactccttg
     2401 tggatgaccg cctcggcgac cttgagctcg accgcgatct tgctgaacgt gacctcggag
     2461 tagccgcggt cgaactcgcg catgatgccc tcgacgccct tggtgtagcc ggtcgccacc
     2521 acgacctgtt cgcgcggatc gatggcctgg aacgacgcgc ggatccggtc gtcgatcgtg
     2581 agcggatcgt cgtcgtggaa gccggtcagg tccatcaggt aagcgcgcac gcccttggcc
     2641 ttgaggatct cgacgctgtt gaacgcgctg tgcgcctcgc cgatcgcggc gagcatctcg
     2701 cgcgcggcca gcagcaggct gtcgcggtgg acgtacccgc tcgccatcac gtggcgcatc
     2761 gagtcgaggt agccgcgcag cgtcgccatc cggcgctcga tgaagccgtt ggccacgtcg
     2821 agcggcaggc cgaccggcgc cagctcgtgg ttgatcgcga ccagccgcgc cgtgagcgcg
     2881 tccagcgccg ccgcgtagtc ggtgccggcc gcgaaccggg cgtagatccc cggctcgccg
     2941 gtcttcttgt gctcgaggag ctggttggtc acgccggcgt acgccgagac gacgtacacg
     3001 cggccgtaca cgtggtgggg gtcgcgcagc atgatgttgc gcagcacgtc
//
LOCUS       DASVOR010000277         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_440799,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000277 DASVOR010000000
VERSION     DASVOR010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440799"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VF516_05855"
     CDS             <1..287
                     /locus_tag="VF516_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX8107232.1"
                     /translation="CATIHRFQGRECDVVILDLVDAAPMRPGLLLSGGLRSDAAQLLN
                     VSLSRARGKLILVADVGYFEAQAPGSAVTAILGEASRSGARVDLAARWDR"
     gene            complement(330..695)
                     /locus_tag="VF516_05860"
     CDS             complement(330..695)
                     /locus_tag="VF516_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107233.1"
                     /translation="MIGRSDVEQALLEDEAVLEQVARLLEARAEFASWPDDLRTALVL
                     ALADAAMSRAESWKAVVLRRHLFGPADRIPDELTVQREPSSRDRRRAERLRSGVPAVV
                     RYRAGVHLLKLDGASRSVP"
     gene            complement(695..1162)
                     /locus_tag="VF516_05865"
     CDS             complement(695..1162)
                     /locus_tag="VF516_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107234.1"
                     /translation="MPEISADAVEIVEQELRARHGPSFRLDVTTGENVWGTILVRDIA
                     TSPVLGTISGLELRALRIEDLFLLKLAAGRAQDLEDLELLALKTTAEALVDRWNQLIK
                     WHGDRHAILGFADALVAQLQQRYGCEPVAIVGRLDVTVGQRELLLESYHGNGS"
     gene            complement(1443..1886)
                     /locus_tag="VF516_05870"
     CDS             complement(1443..1886)
                     /locus_tag="VF516_05870"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HEX8107235.1"
                     /translation="MVALFILLIGAVAVVASLTLMSASRKARGWPVVTGRIVERSIGP
                     STTTGASRPGRYFEPRVTYDYTVDGKAYQGHRIGMTAEAYDEGKARRVVDELPDTVEV
                     HYDPSNPSDAVLQPSAIGMSLLILIFGAIAVVVGGGMLFASFTSK"
     gene            2196..>2858
                     /locus_tag="VF516_05875"
     CDS             2196..>2858
                     /locus_tag="VF516_05875"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8107236.1"
                     /translation="MNTAIQNNDLHGSDSAGRSLGGTDSAPGKRTLTESLDGSSSSSS
                     LEGAAAPVQRKAAPYASPALSAFSVGAVASSFASSGADVAGQVGGSLPSVQRTSAADV
                     AEGGFAGGGHEVPHRAQMEKSFGMDFGHVRAHSDSPATKANDTLGSHAYAMGNQVAFK
                     SASPSPSLVAHELTHVVQQTQSPSFKADGAVDTSGEAEANAVESAVAAGKPASSALRS
                     VTP"
BASE COUNT          433 a         1061 c          965 g          399 t
ORIGIN      
        1 agtgcgccac gatccatcgc ttccagggcc gcgagtgcga cgtcgtgatc ctcgacctcg
       61 tcgacgccgc gccgatgcgc cccggcctgc tgctgtccgg cggcctgcgg tccgacgccg
      121 cgcagctgct caacgtcagc ctgtcgcgcg cccgcggcaa gctgatcctc gtcgccgacg
      181 ttggctactt cgaggcccag gcgcccggca gcgcggtcac cgcgatcctc ggcgaggcca
      241 gccgcagcgg cgcccgcgtc gacctggcgg cgcgctggga ccggtagggc atggcgccga
      301 aggccgatca ccctatgagc tacgagctgc tagggtacgc tgcggctcgc cccgtccagc
      361 ttcagcaggt gcacgcccgc gcggtaccga acaacagcgg gcacaccaga gcgcagccgc
      421 tcggcacgcc tgcggtcccg ggaggacggt tcccgctgca cggtgagctc gtcgggaatg
      481 cggtccgccg gaccgaacag gtgccgtcgc agtaccaccg ccttccagct ctccgcgcgc
      541 gacatggccg catcggcaag cgccaagacg agcgccgtgc gcagatcgtc aggccagctc
      601 gcaaactctg cgcgggcttc gagcaggcgg gcgacctgtt cgagcaccgc ttcgtcttcg
      661 agcagcgctt gttcgacgtc ggagcgcccg atcatcagga tccattgcca tgatagctct
      721 cgagcagcaa ctcgcgctgc cccaccgtca cgtcgagccg gccgacgatg gcgaccggct
      781 cgcagccgta ccgctgttgc aactgcgcca ccagcgcgtc ggcgaagcca agaatggcat
      841 gtcggtcacc gtgccacttg atgagctgat tccagcgatc caccagcgcc tccgcggtcg
      901 tcttcagcgc gagcagctcc agatcctcca ggtcctgcgc ccgcccggca gccagcttca
      961 gcaggaacag atcctcgatg cgaagcgccc ggagctccag gcccgagatg gttccgagca
     1021 cgggcgacgt tgcgatgtcg cgcacgagga tggtccccca cacgttctcg cctgtggtca
     1081 cgtcgagccg gaagctcgga ccgtggcgcg cccgcagctc ttgctcgacg atctccaccg
     1141 cgtctgccga gatctccggc atgtacagat cgacgtcgcg cgagtacgcg cggatctgcc
     1201 atcccgccag cgcggtccct ccgaccagca cgatgggcga gcgcagcact tccccgcgag
     1261 accgcgcgac ttcgacaagg cgcgcaatgg cggcgagcgc gtcgtcgtag gagaggagct
     1321 tgcccggatc cgtgatgtaa ccatcgtcgg ttcgtcgagg ggaccggtca aggtcccggc
     1381 gctgctggtt tcgccgagat cgtggccgca gccgagactc gcgaagcccg cgcgccgctg
     1441 cgctacttgc tggtgaaact cgcgaacaac atcccgccac cgaccacgac ggcgatcgcg
     1501 ccgaagatca ggatcaggag gctcatgccg atcgccgacg gctgcagcac agcgtccgag
     1561 ggattgctcg ggtcgtagtg gacctcgacg gtgtcgggca gctcgtcgac cacgcggcgc
     1621 gccttgccct cgtcgtaggc ctcggccgtc atgccgatcc ggtggccctg gtaggccttg
     1681 ccgtcgaccg tgtagtcgta ggtgacgcgc ggctcgaagt agcgccccgg ccgcgacgcg
     1741 ccggtcgtcg tcgacgggcc gatcgagcgc tcgacgatcc ggccggtcac cacgggccag
     1801 ccgcgcgcct tccggctggc cgacatcagc gtcaggctcg ccacgaccgc caccgcaccg
     1861 atcagcagga tgaagagggc caccatgcgt cgtctcactt tccacctccg ctgaccccgg
     1921 cgcgcggcgc gcgcggcaga agctcgggcg ggctcggcat cggctaggta tatcgcaacg
     1981 cccggcaggc cggtcgaccg ctcggccacc ggtcggacca cgggtcaagt cgccacgttc
     2041 cgtgaccgat ccggcacctc cccgcggcgc ggcggccaat tttgccgtcg cgccatgccc
     2101 agccggggat ggaaacgacg gccacccacg tagtacgccc gagcgccagg tagggggcgg
     2161 ggccggagac gctcgaccct cggacgaggc ccacgatgaa cactgctatc cagaacaatg
     2221 acttgcacgg ctcggacagc gccggtcggt cgctcggggg cacggactcc gctcccggca
     2281 agcggacgct gaccgagtcg ctggacggct cttcgtcctc ttcgtccctg gagggcgcgg
     2341 cggcgccggt gcagcgcaag gccgccccgt acgcgtcgcc cgcgctgagc gcgttctcgg
     2401 tcggcgcggt cgccagctcg ttcgcgtcgt cgggcgccga cgtcgcgggc caggtcggcg
     2461 gcagcctgcc gagcgtgcag cgcacgtcgg ccgccgacgt cgccgagggc ggcttcgcgg
     2521 gcggcggcca cgaggtcccg caccgcgcgc agatggagaa gtcgttcggc atggacttcg
     2581 gccacgtccg cgcccacagc gactcgccgg cgaccaaggc caacgacacg ctcggctcgc
     2641 acgcctacgc gatgggcaac caggtcgcgt tcaagtcggc gtcgccgtcg ccatcgctgg
     2701 tcgcccacga gctgacccac gtcgtccagc agacccagag cccgtcgttc aaggccgacg
     2761 gcgccgtcga cacctccggc gaggccgagg ccaacgccgt cgagtcggcg gtcgccgcgg
     2821 gcaagccggc gtcgagcgcg ctgcgcagcg tcaccccg
//
LOCUS       DASVOR010000278         2717 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353279,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000278 DASVOR010000000
VERSION     DASVOR010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353279"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="VF516_05880"
     CDS             <1..530
                     /locus_tag="VF516_05880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107237.1"
                     /translation="TQVESPWDPSSEADTVRGGPAPKGFTRPMTPPASARPRRSSTPV
                     PGILPIEGSAHRRTDPEIETYLELEADLPPAPGAERDLESEAALNALGADLEEHESVS
                     ITIEEEGRRVIETTTETETHTLTVTVTEAVNSESQHPITAEDGAEVSGSISIPEDPPP
                     SRRQRARRQSDGWDD"
     gene            651..1604
                     /locus_tag="VF516_05885"
     CDS             651..1604
                     /locus_tag="VF516_05885"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8107238.1"
                     /translation="MATQVILFLAANPRGSDPRKLDRECAELQRELKMAPHRNDFRFE
                     SRWAVGIDELMRHLNELDPTVIHFSGHGSGSTGLMLEDERGEPQPVLARALTDMVGST
                     VSKTRAVLLNACYTTEQAEALRDRVDCVVGMDGAIGDDAARAFAIRFYGALGNRRSIG
                     NAVDQGKAALAARQLPDEVLPRCLTRDGIDAHQVFLDPRWPRPCRQGRRGRGAHPMGR
                     RHPPWRRPAASTPRRRVDGAATGGVILRSSRAPGAAPLAMSALPTRHLGHDPAPSTPR
                     AAFVPRLQMPGACLDLTTRCGPRRCFRRCMAGCSPAMPPMR"
     gene            1514..>2717
                     /locus_tag="VF516_05890"
     CDS             1514..>2717
                     /locus_tag="VF516_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107239.1"
                     /translation="MPGPDDAVRAQALLQEMHGWLLAGYAADAVIRAAAVVELALELL
                     VAMPPGAAAGLGALIDELRRSDRTELLVEASWLYQHRITVEPLAGLVRAECDADGRRA
                     CAIASRIAQTAGLVTASQAAACEVAAAHEASASVSSALLRLDRASHRTELDDWLQRPP
                     RVLVALIHGEVGQGHDHFAEIMACQLRSTSRGGWRELTVAWPPPSRSLGTRLAMLLDA
                     LAAGLGVPLGVPADDPATPDGARAWRPVLDRIAAAIDAAREPVLLRHTMSWLATRAGT
                     DDALVDAYLRVIWATVAQRSGERVVVGLDVRRAERTGRPLTRTWWAARRELAVARAIA
                     RVLDHHAMPHGGVCFALPELTSIPIADLVQWLRAEGGRDRDTAHAEAAQLVASTRGGR
                     FDLVIQRLT"
BASE COUNT          361 a          986 c         1014 g          356 t
ORIGIN      
        1 gcacgcaggt cgagtcgccg tgggatccca gcagcgaggc cgacacggtg cgcggcggac
       61 cggcccccaa gggcttcacc cgcccgatga cgccgccggc cagcgcccgg ccgcggcgct
      121 cgtcgacgcc ggtcccgggg atcctcccga tcgagggctc ggcacaccgg aggaccgatc
      181 cggagatcga gacctacctc gagctcgagg ccgatctgcc gcccgcgccc ggcgcggagc
      241 gcgatctcga gtccgaggcc gcgctgaacg cgctgggcgc cgacctcgag gagcacgagt
      301 cggtgtcgat cacgatcgag gaggagggcc ggcgggtgat cgagaccacc accgagaccg
      361 agacccacac gctgaccgtg acggtgaccg aggcggtgaa cagcgagtcc cagcacccga
      421 tcaccgccga ggatggcgcg gaggtctcgg gatcgatctc gatccccgag gacccgccgc
      481 cgagccgccg ccagcgcgcc cggcgccaga gcgacggctg ggacgactga gccggagact
      541 cacgacggcg tcgagcaagc catcggaatg gctggcttcg gggaccccgg cgaatcgctt
      601 ccggagcttg tcgtgatcat ggaggaccgg tcgcctatcg agcgggtggc atggcgacgc
      661 aggtgatcct ctttctggcc gcgaacccgc gcggcagcga tccgcgcaag ctggaccggg
      721 agtgcgccga gctccagcgc gagctcaaga tggcgccgca ccgcaacgac ttccggttcg
      781 agtcgcgctg ggcggtcggc atcgacgagc tgatgcggca cctgaacgag ctggatccga
      841 cagtgatcca cttcagcggc cacggtagcg gaagcaccgg gctgatgctg gaggacgagc
      901 gcggcgagcc gcagccggtg ctggcgcgtg cgctcacgga catggtcggc agcacggtga
      961 gcaagacgcg ggccgtgctg ctcaacgcct gctacacgac cgagcaggcc gaggcgctgc
     1021 gcgaccgggt cgactgcgtg gtgggcatgg acggcgcgat cggtgacgac gcggcgcgcg
     1081 cgttcgcgat ccggttctac ggcgcgctgg gcaaccggcg atcgatcggc aacgcggtgg
     1141 atcaggggaa ggcggcgctc gcggcccggc agctgcccga cgaggtgctg ccgcgatgct
     1201 tgacccggga cggcatcgac gcccatcagg tattcctgga ccctcggtgg ccccgtcctt
     1261 gccgccaggg gcggcgggga cggggagctc atccgatggg caggcggcat cccccatggc
     1321 gccgcccggc cgcgtcgacg cctcgtcgcc gcgtggacgg cgcagccact gggggcgtca
     1381 tcctgcgctc gtctcgcgca ccgggcgccg ctccgctcgc gatgtccgcc ttgccgacac
     1441 ggcatctcgg tcacgacccg gcgccgagca cgccacgcgc ggccttcgtc ccgcggctgc
     1501 agatgcccgg cgcatgcctg gacctgacga cgcggtgcgg gcccaggcgc tgcttcagga
     1561 gatgcatggc tggctgctcg ccggctatgc cgccgatgcg gtgatccgcg ccgccgcggt
     1621 ggtcgagctc gcgctcgagc tgctcgtcgc gatgccgccg ggtgctgccg cagggctcgg
     1681 cgcgctgatc gatgagctgc ggcggtccga ccgcaccgag ctgctcgtgg aagcgagctg
     1741 gctgtatcag caccggatca cggtcgagcc gctcgccggg cttgtccggg cggagtgcga
     1801 cgccgacggc cgccgcgcgt gcgcgatcgc gagccggatc gcccagaccg ccggtctggt
     1861 caccgcgtcg caggccgcgg catgcgaggt cgccgctgcg cacgaggcgt cggcttccgt
     1921 gtcgagcgcg ttgctccggc tcgatcgcgc cagccatcgc acggagctcg acgactggct
     1981 ccagcggccg ccgcgcgttc tggtggccct gatccacggc gaggtcggcc agggccacga
     2041 tcacttcgcc gagatcatgg cctgccagct gcgctcgacg tcgagaggcg gctggcgcga
     2101 gctcaccgtc gcgtggccgc cgccatcgcg ctcgctcggc acgcggctcg cgatgctgct
     2161 cgacgcgctg gccgccggcc tgggggtccc gctcggtgta cccgccgatg atccggccac
     2221 ccccgacggt gcgcgggcgt ggcggccggt gctagatcgg atcgccgcgg cgatcgatgc
     2281 ggcccgcgag cccgtgctgc tccgccacac gatgagctgg ctcgcgaccc gtgcggggac
     2341 cgacgacgca ctggtcgatg cctaccttcg cgtgatctgg gcgaccgtcg cgcagcggtc
     2401 cggcgagcgg gttgtcgtcg gcctcgacgt gcgtcgggcc gagcgcaccg gccggccgct
     2461 gacccggacg tggtgggcgg cgcgccgcga gctcgccgtg gcgcgggcga tcgcacgggt
     2521 cctcgaccat cacgcgatgc cgcacggcgg cgtgtgcttc gcgctcccgg agctgacctc
     2581 gatcccgatc gcggatctcg tccagtggct gcgggccgag ggcggccgcg accgcgacac
     2641 cgcccacgcc gaggccgccc agctcgtggc gtccacccgc ggcggccggt tcgacctcgt
     2701 catccagcgc ctgaccg
//
LOCUS       DASVOR010000279         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_324004,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000279 DASVOR010000000
VERSION     DASVOR010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324004"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VF516_05895"
     CDS             complement(<1..636)
                     /locus_tag="VF516_05895"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8107240.1"
                     /translation="MTDAPRPEARAVRTPPDPRRARARHFVILLGWVSLFADLCYEGM
                     RGAIGGYLAALGASAVAVGMVAGTGEAIGYGLRYVSGALADRTQRYWALTIAGYATNL
                     VAVPLLALAGSWPTVALLVGLERLGKAVRSPAKSTLTSFAASDLGAGKAFAINEAMDQ
                     IGGLAGPLAVTGVLLWRGETLSGYAWAFSLLAIPAAITIAVLLRARRLYPDP"
     gene            complement(633..1571)
                     /locus_tag="VF516_05900"
     CDS             complement(633..1571)
                     /locus_tag="VF516_05900"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8107241.1"
                     /translation="MTGTILRRAGWTVVVVWFAVTATFLVTIAIPADPARALVGPHGT
                     AEAIERIHAHYCLDRGVVGQYGCWIARLAHGDLGESYRSKRAVTEIIADRVWPTAQLA
                     LAAIALQLVLGVPLGIVAARRHGSWQDHGVRLVSSIAQSVPAFVAGTVLLYVVAYRWG
                     WFPIGGYGAGGWDRIAHLALPAMTLASVGVAYYARLVRTELIDVLREDYVRTARAKGL
                     PERVVIGRHALRNALGPLATLVGLDLGVLLGGAVVIELIFAWPGLGRELLQAILELDI
                     PLILGVVMVSACAIAIANLIVDLVQLWLDPRLRDPA"
     gene            complement(1568..>2663)
                     /locus_tag="VF516_05905"
     CDS             complement(1568..>2663)
                     /locus_tag="VF516_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8107242.1"
                     /translation="ILTMPFTTPQRPEHVAAAGDQLRRRPDATGPFELDSWDEGNRIV
                     LRRNPHYVRPERAHLDAIVMLENIPRDTQFQMFERGELDATEKLAAPDYLFVMSEPAW
                     QPCVHQTPVMNVFGSRMNVQVKPFDDRRVRQALNYALDKRHTARLLNGTTVPSHGILP
                     PGMLGRDPGLAPYPHDAAKARALLAEAGYPHGFDVEYLTVHDEETEKVAGSLQGDLAE
                     VGVRVHITVMSLVAYQTAVGQPTGPAFSFTSWTADYPDPTNFFDPRFHSRSIKPTNST
                     NDSFYGNPELDAVLDAARAELDPARRDALYRRAERILYDDAPWIWDYHRLITEVTQPY
                     VRGYEPHPIWNRDFTSAWLDLGPGGAPVPR"
BASE COUNT          382 a          919 c         1013 g          349 t
ORIGIN      
        1 cggatcgggg tacagccggc gggcgcgcag cagcacggcg atcgtgatcg ccgccgggat
       61 tgcgagcagg ctgaacgccc acgcgtagcc ggacaaggtc tcgccgcgcc atagcaggac
      121 gccggtgacc gcgagcgggc cggcgagccc gccgatctgg tccatcgcct cgttgatcgc
      181 gaacgccttg ccggcgccga ggtccgacgc ggcgaacgag gtcagcgtcg actttgccgg
      241 cgagcgcacc gccttgccga gccgctccag gccgaccagc agcgcgacgg tcggccagct
      301 gccggcgagc gcgagcagcg gcacggcgac caggttggtc gcgtagccgg cgatggtcag
      361 cgcccagtag cgctgggtgc ggtcggcgag cgcgccgctg acgtagcgca ggccgtagcc
      421 gatcgcctcg ccggtgccgg cgaccatgcc gaccgcgacg gcgctggcgc cgagcgcggc
      481 gaggtagccg ccgatcgcgc cgcgcatgcc ctcgtagcac aggtcggcga acaggctgac
      541 ccagccgagc aggatcacga agtggcgggc gcgggcccgc cgcggatcgg gcggagtgcg
      601 tacggcgcgc gcctcgggcc gtggtgcatc ggtcatgccg gatctcgcaa tctcgggtcc
      661 agccagagct ggaccaggtc gacgatcagg ttggcgatcg cgatcgcgca cgcgctcacc
      721 atcacgacgc cgaggatcag cgggatgtcg agctcgagga tcgcctggag gagctcgcgg
      781 ccgagccccg gccacgcgaa gatcagctcg atcacgaccg cgccgccgag caacaccccg
      841 aggtcgaggc cgaccagcgt cgcgagcggg cccagcgcgt tgcgcagcgc gtggcggccg
      901 atcacgaccc gctcgggcag gcccttggcg cgcgcggtgc gcacgtagtc ctcgcgcagc
      961 acgtcgatca gctcggtgcg gaccagccgc gcgtagtacg ccacgccgac cgaggccagc
     1021 gtcatcgcgg gcagcgcgag gtgggcgatc cggtcccagc cgccggcgcc gtagccgccg
     1081 atcgggaacc agccccagcg ataggcaacg acgtagagca gcacggtccc ggcgacgaac
     1141 gccggtacgc tctgcgcgat cgagctcacc aggcggacgc catgatcctg ccagctcccg
     1201 tgccggcgcg cggcgacgat gccgagcggg acgccgagca cgagctggag cgcgatcgcg
     1261 gcgagcgcga gctgcgcggt cggccacacc cggtcggcga tgatctcggt gaccgcgcgc
     1321 ttgctgcggt agctctcgcc gaggtcgccg tgcgcgagcc gggcgatcca gcacccgtac
     1381 tggccgacca cgccgcgatc gaggcagtag tgcgcgtgga tccgctcgat cgcctcggcg
     1441 gtgccgtgcg ggccgaccag cgcgcgggcc ggatcggccg ggatcgcgat cgtcaccagg
     1501 aacgtcgcgg tgaccgcgaa ccagaccacg accaccgtcc agccggcgcg gcgcaggatc
     1561 gttccggtca tcgcggcacc ggcgcgccgc ctggcccgag atcgagccac gcggacgtga
     1621 agtcgcggtt ccagatcggg tggggctcgt agccgcgcac gtagggctgg gtgacctcgg
     1681 tgatcagccg gtggtagtcc cagatccacg gcgcgtcgtc gtacaggatc cgctcggcgc
     1741 ggcggtacag cgcgtcgcgc cgggccgggt cgagctcggc ccgcgcggcg tcgagcaccg
     1801 cgtcgagctc ggggtttccg tagaacgagt cgttggtcga gttggtcggc ttgatcgacc
     1861 gcgagtggaa ccgcggatcg aagaagttgg tgggatccgg gtagtcggcg gtccagctcg
     1921 tgaacgagaa cgccggcccg gtcggctggc cgaccgcggt ctggtaggcc accagcgaca
     1981 tcaccgtgat gtggacccgg acgccgacct cggcgagatc gccctgcagc gagccggcga
     2041 ccttctcggt ctcctcgtcg tggaccgtca ggtactcgac gtcgaacccg tgcgggtagc
     2101 cggcctcggc gagcagcgcg cgcgccttcg cggcgtcgtg cgggtagggc gcgaggccgg
     2161 gatcgcggcc gagcatcccg ggtggcagga tgccgtgcga cggcacggtc gtgccgttga
     2221 gcagccgcgc ggtgtggcgc ttgtcgagcg cgtagttgag cgcctggcgg acccgtcgat
     2281 cgtcgaacgg cttgacctgc acgttcatcc gcgagccgaa cacgttcatc accggggtct
     2341 ggtggacgca gggctgccac gccggctcgc tcatcacgaa caggtagtcg ggcgccgcga
     2401 gcttctcggt ggcgtcgagc tcgccgcgct cgaacatctg gaactgggtg tcgcgcggga
     2461 tgttctccag catcacgatc gcgtcgaggt gcgcgcgctc gggccggacg tagtgcgggt
     2521 tgcggcgcag cacgatccgg ttgccctcgt cccagctgtc gagctcgaac ggcccggtgg
     2581 cgtcgggccg gcgccgcagc tggtcgcccg cggcggcgac gtgctcgggg cgctgcgggg
     2641 tcgtgaacgg catcgtcagg atg
//
LOCUS       DASVOR010000280         3051 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_294850,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000280 DASVOR010000000
VERSION     DASVOR010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294850"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..243)
                     /locus_tag="VF516_05910"
     CDS             complement(<1..243)
                     /locus_tag="VF516_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107243.1"
                     /translation="MTGAPAARPESRGGAQEVVRRPGIARRTGQNPAVAELQEHCVNK
                     IFLIALCAGLVACMNRKKEEEGREPVQTAPAPGPAGG"
     gene            complement(240..1013)
                     /locus_tag="VF516_05915"
     CDS             complement(240..1013)
                     /locus_tag="VF516_05915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107244.1"
                     /translation="MRVLLVVWAVAAVGCGGHPPIPQRGVVESDLGSWKFRRFQGPLL
                     DVEVWVEGNKAEAYSASYITADAEKRGRIEDKDLVNVFVTRYESPDGVVRATVKLARR
                     LAADNGYQVEEDKIAGGRALSITGRGEAWVMWPSSRYVVKVGGRGRSDVPASMVEPYV
                     DRYPSQLPGGSLEGPLPPGRDAPPATHKLDKPAYDPSNPRPDAEHYDPNKVKLPDRSD
                     RGDPGNPAPGADSKPAADDADAARPAKPADTKKKKPRSR"
     gene            1083..2318
                     /gene="metK"
                     /locus_tag="VF516_05920"
     CDS             1083..2318
                     /gene="metK"
                     /locus_tag="VF516_05920"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEX8107245.1"
                     /translation="MASYLFSSESVTEGHPDKLCDQVSDAVLDACLAVDPQSRVACES
                     VAKTGFVLVAGEITTKAQLDFPRIVRGVVRDIGFTSSQIGFDADTCAVIVAVEQQSPD
                     IAQGVDRGDPAKQGAGDQGLMFGYACDETKELMPLPIQMAHQLAMKLAEVRKKKTRGI
                     DFLRPDGKTQVSVRYEDGEPTGVEAIVVSTQHDDKVKQKTIEEAIREHVIKPIVPARL
                     VSNKTKIHVNPTGRFVIGGPKGDTGLTGRKIIVDSYGGYARHGGGAFSGKDPSKVDRS
                     AAYYARYIAKHIVAAGLARRCEVQFAYAIGVAEPVSMLVDTFGTGVVADDKLTRAVLA
                     TFDARPGMLIKELDLRRPIFRKTAAYGHFGRDDADFTWEKTPLLDKLKDAAHSGNGVA
                     NGKPAAKKPRAKPGAAARV"
     gene            complement(2315..2779)
                     /locus_tag="VF516_05925"
     CDS             complement(2315..2779)
                     /locus_tag="VF516_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107246.1"
                     /translation="MRTLLVSVLVLAAAACSSKSHDETLSASDSRDVLINRNWIDRMP
                     ERTTDRLHVYRFVPTMGGGVYQDRTLFKGTFELFMFKVEGGDHIVFQLPETHEKVTSQ
                     FQIERVAGPRPFDLKLTIFSDPRGPHVYYGIRAETDSDGHLLEQRLAALKGN"
BASE COUNT          451 a         1080 c         1055 g          465 t
ORIGIN      
        1 gccccccgcc ggcccgggcg ccggcgcggt ctgcaccggc tccctgccct cctcctcctt
       61 cttccgattc atgcaggcca cgaggccggc acacaacgcg atcagaaaga tcttgttcac
      121 gcagtgctcc tggagttccg cgacggctgg attctggccc gtccgcctcg cgatgccagg
      181 gcggcgcacc acttcttgcg ctccgccgcg cgattccggc cgggcggccg gggcgccggt
      241 cacctggagc gaggcttctt cttcttggtg tcggccggct tggcgggcct ggccgcgtcg
      301 gcgtcgtccg cggccggctt gctgtcggcg cccggtgccg ggttgcccgg gtcgccgcgg
      361 tcgctgcgat ccgggagctt caccttgttc gggtcgtagt gctcggcgtc gggccgcggg
      421 ttgctcggat cataggccgg cttgtccagc ttgtgggtcg cgggcggcgc gtcgcggccc
      481 ggcggcagcg gcccctcgag cgatccgccg ggcagctgcg acgggtagcg gtcgacgtag
      541 ggctcgacca tcgacgccgg gacgtccgag cgcccgcgcc cgccgacctt cacgacgtag
      601 cggctcgacg gccacatcac ccaggcctcg ccccggccgg tgatgctgag cgcgcgaccc
      661 ccggcgatct tgtcctcctc gacctgatag ccgttgtcgg ctgcgagcct gcgcgccagc
      721 ttcacggtgg cgcgcaccac gccgtcgggc gactcgtagc gggtcacgaa cacgttcacc
      781 aggtccttgt cctcgatccg cccgcgcttc tcggcgtcgg ccgtgatgta gctcgcgctg
      841 taggcctcgg ccttgttgcc ctcgacccag acctcgacgt cgagcagcgg gccctggaac
      901 cgccggaact tccacgatcc caggtcggac tcgaccacgc cgcgctgggg gatcggggga
      961 tgaccgccgc agccaaccgc cgccacggcc cagacaacga gcagcacacg catggtcggc
     1021 cccatcgtac cccctttgca gcgttgacag cggagtcccg tcactgaata gtcccgccac
     1081 ccatggccag ctatctgttc tcgtccgaat ccgtcaccga agggcacccc gacaagctgt
     1141 gcgaccaggt gagcgacgcg gtcctcgatg cttgcctcgc ggtcgatccg cagagtcgcg
     1201 tcgcgtgcga gtcggtggcc aagaccggct tcgtgctggt cgccggcgag atcaccacca
     1261 aggcgcagct cgacttcccg cgcatcgtgc gcggcgtggt ccgcgacatc ggcttcacga
     1321 gctcgcagat cggcttcgac gccgacacct gcgccgtgat cgtcgcggtc gagcagcagt
     1381 cgccggacat cgcgcagggc gtcgaccgcg gcgatccggc caagcagggc gccggcgacc
     1441 agggcctgat gttcggctat gcgtgcgacg agaccaagga gctgatgccg ctgccgatcc
     1501 agatggcgca ccagctcgcg atgaagctcg ccgaggtccg caagaagaag acccggggca
     1561 tcgacttcct gcgccccgac ggcaagaccc aggtctcggt ccgctacgag gacggcgagc
     1621 ccaccggcgt cgaggcgatc gtcgtgtcga cccagcacga cgacaaggtc aagcagaaga
     1681 cgatcgagga ggcgatccgc gagcacgtga tcaagccgat cgtcccggcc cggctggtga
     1741 gcaacaagac caagatccac gtcaacccca ccggccggtt cgtgatcggc ggcccgaagg
     1801 gcgacaccgg cctgaccggc cgcaagatca tcgtcgacag ctacggcggc tacgcccgcc
     1861 acggcggcgg cgcgttctcc ggcaaggacc cgagcaaggt cgaccgctcg gccgcgtact
     1921 acgcgcgcta catcgccaag cacatcgtcg cggccggcct ggcccggcgc tgcgaggtcc
     1981 agttcgccta cgcgatcggg gtcgccgagc cggtctcgat gctggtcgac acgttcggca
     2041 ccggcgtggt cgccgacgac aagctgaccc gggcggtgct cgcgacgttc gacgcccgcc
     2101 ccggcatgct gatcaaggag ctcgacctgc gccggccgat cttccgcaag accgcggcct
     2161 atggccactt cggccgcgac gacgccgact tcacgtggga gaagaccccg ctgctcgaca
     2221 agctcaagga cgccgcacac agcggcaacg gcgtcgccaa cggcaagccc gccgccaaga
     2281 agcccagggc caagccgggc gccgcggccc gggtctagtt gcccttcagc gcggccagcc
     2341 gctgctcgag caggtggccg tcgctgtcgg tctcggcgcg gatgccgtag tagacgtgcg
     2401 ggccgcgcgg atcgctaaag atcgtgagct tgaggtcgaa cggccggggg ccggcgacgc
     2461 gctcgatctg gaactggctg gtgaccttct cgtgggtctc gggcagctgg aacacgatgt
     2521 gatcgccgcc ctcgaccttg aacatgaaca gctcgaacgt gcccttgaac agcgtgcggt
     2581 cctggtagac gccgccgccc atggtgggca cgaagcggta gacgtgcagc cggtcggtgg
     2641 tccgctccgg catgcggtcg atccagttgc ggttgatcag gacgtcgcgg gagtcgctcg
     2701 ccgacagcgt ctcatcgtgg gacttcgacg agcaggcagc ggcggcgagc acgaggaccg
     2761 acacgagcaa ggtgcgcatg gtcacatggt agctgcaagt cgcgcgctcg gcagctggcc
     2821 ggcgcacggt aggtgcagga cagggcaggc gcccgggaga acgcgtgcac gctcggctcg
     2881 ccggggctgc tgcgcggacg ggtgtcggag ccgcgcccgc cgaatcacca cccgtcctgc
     2941 gcgggcggcg cgactatgtt gtggcggtcg tgtcaagcgc agatctctct tgtccggatg
     3001 gttctcctcc ggtctgtcgg gttggctgcg gttccaggag tggctggcgt g
//
LOCUS       DASVOR010000281         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265435,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000281 DASVOR010000000
VERSION     DASVOR010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265435"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF516_05930"
     CDS             complement(<1..313)
                     /locus_tag="VF516_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX8107247.1"
                     /translation="MGTKLYVGNLNYNTNEESLREAFSANGREVTSVSIIMDRETGRS
                     RGFAFVEMSTAEFAQQALQELDGQELDGRMLRINEAREREPRGPGGPPRSYGPRPGGG
                     GG"
     gene            499..1161
                     /locus_tag="VF516_05935"
     CDS             499..1161
                     /locus_tag="VF516_05935"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX8107248.1"
                     /translation="MAAVLAGRGQAADRAAAAPAASPMADITDVYAWMAGSNLNLVMD
                     VSPQDNGTNAFGPDILYAFHLTSKSGLGAPGGTETQVICRFASSTSAQCWVVSGGATK
                     AYVTGDPSNTAGVTSPSGKVRVFAGRRSDPAFFNLSGFNTAMAGFAPQLGVPNITDAA
                     GCPTGVPQDRFGTLRTQLGTGSDSFAASNVMAIVVQIDKALVNTANNTVIAVWGSTHA
                     GA"
     gene            1192..1884
                     /locus_tag="VF516_05940"
     CDS             1192..1884
                     /locus_tag="VF516_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107249.1"
                     /translation="MTGMIVLAGCGGQSGDAVHHDGGNLDSAPIPPPPVLGAQIDRMG
                     RPLIGTALIGTFKSPSDQAMIRDAYNHASDPATWATTTISTNVTIETEMETNLAVFDA
                     FDIADTDIAGAGCGNAVRYQVGPGMSPYRLAADLFADDELYVDTAFTTCSIYLALEMQ
                     FATANGRLHTTCGGRMPSHDVVDITYSMLAAGRDGIDPTSSDGAPKIHDGVAAHTDIK
                     ETVFPFLGPPHP"
     gene            1916..>2269
                     /locus_tag="VF516_05945"
     CDS             1916..>2269
                     /locus_tag="VF516_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107250.1"
                     /translation="MGPLPYPHRATFLATTLTVAPDQDALAPYAARLIAVILFDHLVR
                     HPVVTLGDHDDERLTDDTGRLLDATHPQLEDSIDWFFRVSRRHEVLWFELAIDPEQPG
                     PARPRPAVLRSRRPRG"
BASE COUNT          366 a          817 c          728 g          358 t
ORIGIN      
        1 cgccaccacc cccgccgggg cgcggaccgt acgagcgcgg cggaccaccg gggccgcgcg
       61 gctcgcgctc gcgcgcctcg ttgatccgga gcatccggcc gtcgagctcc tggccgtcga
      121 gctcctggag cgcctgctgg gcgaactccg ccgtcgacat ctcgacgaac gcgaacccgc
      181 gagagcgtcc ggtctcacgg tccatgatga tggacacgct cgtcacctca cgtccgttcg
      241 cgctgaacgc ctctctaagt gattcttcgt tcgtgttgta gttcagattg cccacgtaga
      301 gcttcgttcc cacggacggt ctcctgaaga tgcagccgaa aagtccttga gccgtcccaa
      361 gcctatcacg gcggctggcg ctcagatcac atcccccggt taggaactgc ctcgcccgca
      421 cgtttggggc tagattcgat ggtctagggg gttaccttga agaattttgc ctccctcgga
      481 ggggtagcag ggttagccgt ggcggccgtg ctcgccggcc gaggccaggc cgccgaccgt
      541 gccgcagcag cgccggccgc gagcccgatg gcggacatca ccgatgtcta cgcctggatg
      601 gccggctcga acctgaacct ggtgatggac gtatcaccgc aggacaacgg cacgaacgcg
      661 ttcggcccgg acatcctgta cgcctttcac ctgaccagca agtccggcct cggcgcgccg
      721 ggggggaccg agacccaggt gatctgccgg ttcgcctcga gcaccagcgc gcagtgctgg
      781 gtcgtgtccg gcggcgctac gaaggcctac gtgaccggcg atccgagcaa caccgcgggc
      841 gtgacctcgc cgtccggcaa ggtgcgggtg ttcgccggcc ggcggtcgga tcccgcgttc
      901 ttcaacctgt ccggcttcaa cacggcgatg gcaggcttcg cgccgcagct cggcgtcccc
      961 aacatcactg atgcagccgg ctgtccaacc ggggtccccc aggacagatt tgggaccctg
     1021 cgtacgcaac tgggcaccgg ctccgatagc tttgcagcat ccaacgtcat ggcgatcgtg
     1081 gtccagatcg acaaggcgct ggtcaacacc gcgaacaaca ccgtcatcgc agtctggggc
     1141 tccacccacg cgggggcatg aaccatgcga tccaccacgg cgagatacct cgtgaccggt
     1201 atgatcgtgc tcgctggttg cggcggccag tccggcgacg cggtccacca cgacggcggc
     1261 aacctcgata gcgcaccgat cccaccccca ccagtcctag gcgcgcagat cgaccgcatg
     1321 gggcgcccac tgatcggcac cgcgctgatc ggcacgttca agtccccctc ggatcaggcc
     1381 atgatccgcg acgcctacaa ccacgcctcg gatccggcga cgtgggcgac cacgacgatc
     1441 tccaccaacg tgacgatcga gaccgagatg gagaccaacc tcgccgtgtt cgatgcgttc
     1501 gacatcgcgg acacggacat cgccggcgcg gggtgcggta atgccgtgcg ctaccaagtg
     1561 gggccaggga tgtcgccgta ccgcctggcg gccgacctgt tcgccgacga cgagctctac
     1621 gtcgacaccg ctttcacgac ctgctcgatc tacctggcgt tggagatgca gttcgcaacc
     1681 gcgaacggcc ggttgcacac cacgtgcggc ggccggatgc cgagccacga cgtcgtcgac
     1741 atcacctatt cgatgctcgc ggccggccgc gacgggatcg accccacgag cagcgacggt
     1801 gcgccgaaga tccacgacgg cgtggcggcg cacaccgata tcaaggagac ggtgttcccg
     1861 ttcctcggac cgccgcaccc ctagccccgg tcttgccgta tcctgtgtgc ggtccatggg
     1921 gccgctgccg tatccgcacc gcgcgacgtt cctcgcgacc acgctgaccg tggctcccga
     1981 ccaggacgcg ctggcgccgt acgccgcgcg gctgatcgcg gtgatcctgt tcgaccacct
     2041 ggtccgccac ccggtggtca cgctcggcga tcacgacgac gagcggctga ccgacgacac
     2101 cgggcggctg ctcgacgcga cccaccccca gctcgaggac tcgatcgact ggttcttccg
     2161 ggtgtcgcgc cgccacgagg tgctgtggtt cgagctcgcc atcgatccgg agcagcccgg
     2221 gccggcgcgg ccgcggccgg cggtgctgcg atcgcgccgg ccgcgcggc
//
LOCUS       DASVOR010000282         3583 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207162,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000282 DASVOR010000000
VERSION     DASVOR010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3583
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207162"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..575)
                     /locus_tag="VF516_05950"
     CDS             complement(189..575)
                     /locus_tag="VF516_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107251.1"
                     /translation="MSRYDWFRRRIAPVAFFLAIGLIARDSCEKSHRTHTTVELQLGD
                     ARSRVRAVDVDVVTGDQTVARFHRAALPDTMIGPCRFELALPADDGQLRIDVDLGAEH
                     RRLTRPFHAVEGATMFVPIEGELAPR"
     gene            complement(883..1785)
                     /locus_tag="VF516_05955"
     CDS             complement(883..1785)
                     /locus_tag="VF516_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107252.1"
                     /translation="MSKPGVLLLSLTVFVSLGGSASAQIVLPGLPEVQAVNASAQLGT
                     IWPTRSPSVPSTNVADFDLWGWGVETEFHLPFQDGSKWEASLAVGYNQLSLSADLKGR
                     HLRGTLRDLPALSLYFGRAKRCSLEFRDRFDPSAAVGDQLVKTCSQGGWYWGLTIAES
                     ELINGRLIRTPDPGTVDPGPIKISAGSFSAGVAVGYENGPVFIELGYVGRYFPALAYD
                     GIPAATGFPADLDQRMYAGGFVLRVGATLDLQKPAVPKVAISCAGASTEIKVGSEMDL
                     KHECDGDQLNVHVRKKPAATPPHL"
     gene            2106..>3583
                     /locus_tag="VF516_05960"
     CDS             2106..>3583
                     /locus_tag="VF516_05960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107253.1"
                     /translation="MDTVRARVEQALFGATSQTRLGRYIITERIAGGGMGIVYRAEDP
                     ELHRKVALKVLHPRQLGDARARARMLAEARALARLDHPSVVKIHDVLTIDDQIVLVMA
                     LVEGVTLAAWESAAPRSWREVVRAYAQAGDGLAAAHALDIIHRDFKPANAILGDDGQV
                     RVLDFGLARLADAGDPGAPLGRPDPAASGADDTTRSPSAAGSGPTIHGLGGTTLGALG
                     GPADPIVRGLTATDEVVGTLNYISPEQLAGQPASHASDQFSFCVALHRAVEGVPPFAG
                     ADVASRLRCIRSGAIQRATDRRVPAWLRVVLARGLSSDPVTRFPSMAALLAALRRPRG
                     LRRWRVPLAAAAMAGAFAIAFTGRSSLAALESPCDGGVREIAVIWGAAQRARLDAALR
                     AIDTPDLRAMQDRVMSRLDRYRDRWTELHRDACTRHRRGIQSAALLDRRMACLGRRLD
                     DLGAAVDVLDHTDRATAGHVLDVVAHLPELDTCADAARLEADA"
BASE COUNT          495 a         1404 c         1160 g          524 t
ORIGIN      
        1 cgatctacgt cagctgtggg ccggaggcgc tcggccgcga cctcgtcacc ctggcccgcc
       61 acggctacac gcccgacgcg atcgagccgt tcgacctgat gcccgggacc gcccaggtcg
      121 agaccctggt ccggctgcgc cggccggcgc gcgcgatccg ccgcgcccgc tgaccgccag
      181 cggccgcctc agcgcggcgc gagctctcct tcgatcggca cgaacatcgt cgcgccctcg
      241 acggcgtgga acggccgggt cagccggcgg tgctcggcgc cgaggtcgac gtcgatccgc
      301 agctggccat cgtcggcggg cagcgcgagc tcgaaccggc acgggccgat catcgtgtcc
      361 ggcagcgccg cgcggtggaa ccgggcgacg gtctggtcgc cggtcacgac atcgacatcg
      421 accgcgcgca cccgggaccg cgcgtcgccg agctggagct cgaccgtggt gtgggtgcgg
      481 tggctcttct cgcagctgtc ccgcgcgatc aggccgatcg cgaggaagaa cgcaaccggc
      541 gcgatccggc gccggaacca gtcgtagcgc gacacgccgg ggttctagca caccgccggc
      601 ggcgccgttc cagttcgcgc cctgcggcgg cgatcacgac gcgcgcccgg tggcgtcaga
      661 gtcgctcgct gcagcgttcg gcgccccgcg tcgggaagga cgaccttccg ctacgtcccc
      721 gacgagcacg tcgtaccctc gccgaccttc tggacgacgc gcgcaacgat gtccgcgacc
      781 atgtcgccac ctagcccgca actcggcgtt cttcgcacag tcgcctgccg ccgagcgccg
      841 gacctcgcca cgcgcgccgt ccgccccatc ccgccgccat cgtcacaggt gcggtggtgt
      901 ggccgctggc ttcttgcgca cgtgcacgtt cagctgatcg ccgtcgcact catgcttgag
      961 atccatctcg gagcccacct tgatctccgt gctggcgccg gcacacgaga tcgcgacctt
     1021 cggcacggcc ggcttctgca gatccagcgt ggcgccgaca cgcagcacga acccgcccgc
     1081 gtacatgcgc tgatcgaggt cggcgggaaa cccggtggcg gccgggatcc cgtcgtacgc
     1141 gagggccggg aaataccgtc cgacgtatcc gagttcgatg aacactggac cattctcgta
     1201 tcccaccgcc acgccggcgg agaacgagcc cgccgagatc ttgatgggcc ccggatcaac
     1261 cgtacccgga tcgggcgtgc gaatgagccg cccattgatc agctcggatt cggcgatcgt
     1321 caaaccccag taccatccac cttgactgca cgtcttcacg agctggtcgc ccactgcagc
     1381 cgacgggtcg aagcgatcgc gaaattctag agagcagcgc tttgcacgcc cgaagtacaa
     1441 ggagagtgcg ggcaggtcac gcagcgtgcc acgcaggtgt cggcccttga ggtccgcact
     1501 caggctcagc tggttgtagc ccaccgcgag cgacgcttcc cacttcgagc cgtcctggaa
     1561 tggcagatgg aactcggtct ccacccccca tccccacaaa tcgaagtccg cgacgttcgt
     1621 actgggcacg gacggcgatc gcgtcggcca gatcgtacct agctgcgcag atgcgttcac
     1681 ggcctgcact tccgggagcc ccggcaagac gatctgcgcg gaggccgagc ctcccagcga
     1741 cacgaacacg gtgagcgaga gcagcagcac gcccggcttc gacacggaga cctcctgacc
     1801 ttcccgcaag ctcgcggggg acatctccgt agtgccgtcg ccccgcgacg cattcccgag
     1861 tttctgcagc gccgcttgtg tccgccgctc gggacggctc ccggcttctg cgcatgcccg
     1921 gggcatcttg caccggtcgc gcactcgggt ttctgttgcg cgcgccacgc gatgcgggct
     1981 agcttggtcg caaaggggtc atggcggagc tgcgtcggag cgccgatctg gatgtcgagg
     2041 ttcctcccgc cgatctgccc tcgggcgtcg tccgtggcgc ccgcccggca agcgacgccg
     2101 tcgcgatgga caccgtccgt gcgcgcgtcg agcaggcgct gttcggcgcc accagccaga
     2161 cccggctcgg ccgctacatc atcaccgagc gcatcgccgg cggcggcatg ggcatcgtct
     2221 accgcgccga ggaccccgag ctccaccgca aggtcgcgct caaggtcctc cacccgcgcc
     2281 agctcggaga cgcgcgcgcc cgggcgcgca tgctcgccga ggcccgcgcc ctcgcccggc
     2341 tcgatcaccc gagcgtcgtc aagatccacg acgtcctcac catcgatgac cagatcgtcc
     2401 tggtgatggc gctggtcgag ggcgtcacgc tcgccgcctg ggagtctgcc gcgccgcgat
     2461 cctggcgcga ggtcgtgcgc gcctatgccc aggctggcga tggtctcgcc gccgcccacg
     2521 ccctcgacat catccaccgc gacttcaagc cggccaacgc catcctcggc gatgacggcc
     2581 aggtccgcgt gctcgacttc ggcctggcac gcctcgccga cgctggcgat cccggcgccc
     2641 cgctcggccg cccggatccg gccgccagtg gcgccgacga caccacccgc agccccagcg
     2701 ccgctgggag cggcccgacg atccacggcc tcggcggtac aaccctcggc gctctgggcg
     2761 gccctgccga cccgatcgtc cgcggtctga ccgccaccga cgaggtcgtt ggcacgctca
     2821 actacatctc tcccgagcag ctcgccggcc agcccgcgag ccatgcctcc gaccagttca
     2881 gcttctgcgt tgcgctccac cgcgcggtcg aaggcgtgcc gccgttcgca ggcgccgatg
     2941 tcgcctcgcg cctccgctgc atccgcagcg gcgcgatcca gcgcgccacc gaccgccgcg
     3001 tccctgcctg gctgcgcgtc gtcctggccc gcggcctgtc cagcgatccg gtcacccgct
     3061 tcccctccat ggcggcgctc ctggcagcgc tgcggcgccc acgtgggttg cggcgctggc
     3121 gggttcccct cgccgctgcg gccatggccg gcgcgtttgc gatcgccttc accggccgct
     3181 cctccctggc cgcgctcgag tccccgtgcg acggcggcgt tcgcgagatc gcagtcatct
     3241 ggggcgccgc gcagcgcgct cggctcgatg cagcgctacg cgccatcgac actcccgacc
     3301 tgcgcgccat gcaagatcgc gtgatgtccc ggctcgacag gtaccgcgat cgctggaccg
     3361 agcttcaccg cgacgcctgc acccgccacc gccgcggcat tcagtccgcg gcgctgctcg
     3421 accgccgcat ggcgtgcctc ggccgccggc tcgacgacct cggcgccgcc gtcgacgtcc
     3481 tggaccacac cgatcgcgcc accgcgggcc acgtgctcga cgtcgtggcg cacctccccg
     3541 agctcgacac ctgcgccgac gcggcgcgcc tcgaggccga cgc
//
LOCUS       DASVOR010000283         2508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_2704,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000283 DASVOR010000000
VERSION     DASVOR010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2704"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VF516_05965"
     CDS             complement(<1..988)
                     /locus_tag="VF516_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107254.1"
                     /translation="MDEPINLLLVDDETRNLDALEAVLDDPSYHLLRADNADAALRQL
                     LQNQVAAIVLDIKMPGVSGFELANLIKGTKKFRQIPILFLTAYHVDDQDILAGYSVGA
                     VDYLTKPVKPQILRHKIAVFADLFRKTRALAELNDTLELRVKQRTEELESSEAALRDA
                     NRQKDQFLATLAHELRNPLAPLRTGLDLLMQRPDAPVSASSRVLGAMNRQLDHMVRLI
                     DDLLDVSRISRGVLELRREPADLAVVIGNAIESARPFIANKQQTVVFDNPAAVRVEVD
                     PTRIAQIIGNLLHNASKFSPVGAEIRIGLTADHGGATIRVIDRGIGIPPAEIE"
     gene            complement(1012..>2508)
                     /locus_tag="VF516_05970"
     CDS             complement(1012..>2508)
                     /locus_tag="VF516_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107255.1"
                     /translation="IAPEKQKLIFEAFQQADAGTSRKYGGTGLGLAISRELASLLGGE
                     IKLVSKPGSGSTFTLYLPISYTGPAQRVFTPSTQSPQAAMAMPVLSIAKTEETVPDDR
                     ETIQPGDTVLLICEDDPHYARILLGIARGRGFKGLVANRGQQALTLAREFIPTAITLD
                     VFLPDMLGWTVLNNLKLDPRTRHIPVQMLSVEEERQHGLSHGAFSYLVKPATTGELET
                     ALDRLKTYVAPHTKRLLVVEDNDVERDSIVELLGAGDIEIVGAATGAEALAVLGREPV
                     DCCVVDLRLPDMSGFELLERVQADEAMRNIPVVVFTGKQLSAEEEARLRTVAKSIVLK
                     DVQSPERLLDETSLFLHRVIEDLPDGKRKMIERLHGSTEALRGRKVLVVDDDARNIFA
                     LATVLENQEMEVISAMNGRQAIELIETTPDLSVVLMDIMMPEMDGYETMREIRKDPRF
                     RTLPILALTAKAMKGDREKCLQAGASDYIAKPVNTDQLLSLLRVWLYR"
BASE COUNT          352 a          825 c          886 g          445 t
ORIGIN      
        1 gctcgatctc ggccggtggg atgccgatgc cgcgatcgat cacgcggatc gtcgcgccgc
       61 cgtggtcggc ggtgagcccg atgcggatct cggcgccgac cggcgagaac ttggacgcgt
      121 tgtgcagcag gttgccgatg atctgcgcga tccgggtcgg gtcgacctcg acgcgcaccg
      181 cggcggggtt gtcgaacacc acggtctgct gcttgttcgc gatgaacggc cgggccgact
      241 cgatcgcgtt gccgatcacc accgccagat cggccggctc gcgccgtagc tccagcacgc
      301 cgcggctgat ccgggacacg tcgagcaggt cgtcgatcag ccggaccatg tgatcgagct
      361 ggcggttcat cgcgccgagc acgcggctgg acgccgagac cggagcgtcg ggccgctgca
      421 tgagcagatc gagcccggtg cgcagcggtg cgagcgggtt gcgcagctcg tgggccaggg
      481 tggccaggaa ctggtccttc tggcggttgg cgtcgcgcag cgcggcctcc gagctctcca
      541 gctcctcggt gcgctgcttg acccgcagct ccagcgtgtc gttgagctcg gccagcgcgc
      601 gggtcttgcg gaacagatcg gcgaacacgg cgatcttgtg gcggaggatc tgcggcttga
      661 ccggcttggt caggtagtcg accgcgccca cgctgtagcc ggccaggatg tcctggtcgt
      721 cgacgtggta cgcggtgagg aacaggatcg ggatctggcg gaacttcttg gtgcccttga
      781 tcaggttggc cagctcgaac ccgctgaccc cgggcatctt gatgtcgagc acgatcgccg
      841 cgacctggtt ctggagcagc tggcgcagcg cggcgtcggc gttgtcggcc cgcagcaggt
      901 ggtagctcgg atcgtcgagg accgcctcga gcgcgtcgag gttgcgggtc tcgtcgtcga
      961 cgagtaacag attgatgggt tcgtccatcc gatgtaccga tcttaccgat ctcagcgata
     1021 caaccatacc cggagcagcg acaacaactg atccgtgttg acgggcttgg cgatgtagtc
     1081 ggaggcgcct gcctgcagac acttctcgcg gtcgcccttc atcgccttgg cggtcagcgc
     1141 caggatcggc agtgtccgga accgggggtc cttgcggatc tctcgcatgg tctcgtagcc
     1201 gtccatctcg ggcatcatga tgtccatcag gaccacgctg aggtcggggg tggtctcgat
     1261 cagctcgatc gcctggcggc cgttcatcgc gctgatcacc tccatctcct ggttctccag
     1321 cacggtggcg agcgcgaaga tgttgcgggc gtcgtcgtcg accaccagca ccttgcggcc
     1381 gcgcagcgcc tcggtcgagc cgtgcaaccg ctcgatcatc ttgcgcttgc cgtccggcag
     1441 gtcctcgatg acgcggtgca ggaacagcga ggtctcgtcg agcagccgct cgggggactg
     1501 gacgtccttg agcacgatgc tcttggccac cgtgcgcagc cgggcctcct cctcggcgct
     1561 gagctgcttg ccggtgaaca ccacgaccgg gatgttgcgc atcgcctcgt cggcctgcac
     1621 ccgctcgagt agctcgaagc cggacatgtc gggcaaccgc aggtcgacca cgcagcagtc
     1681 gaccggctcg cgcccgagca cggccagcgc ctcggcgccg gtcgcggccc cgacgatctc
     1741 gatgtcgccc gcaccgagca gctcgacgat gctgtcgcgc tccacgtcgt tgtcctcgac
     1801 cacgagcagc cgcttggtgt gcggcgccac gtaggtcttg agccggtcga gggcggtctc
     1861 gagctcgccg gtggtcgccg gcttgaccag gtacgagaac gcgccgtgcg acagcccgtg
     1921 ctggcgctcc tcctcgaccg acagcatctg gaccgggatg tggcgggtcc ggggatcgag
     1981 cttgaggttg ttgagcacgg tccagcccag catgtcgggc aggaacacgt cgagcgtgat
     2041 cgcggtcggt atgaactcgc gggcgagcgt gagcgcctgc tggccgcggt tggcgaccag
     2101 gcccttgaac ccgcggccgc gcgcgatccc gagcaggatc cgggcgtagt gcgggtcgtc
     2161 ctcgcagatc agcagcaccg tgtcgccggg ctggatggtc tcgcggtcgt ccggaacggt
     2221 ctcctcggtc ttggcgatcg acagcaccgg catcgccatc gccgcctgcg gcgactgggt
     2281 gctcggcgtg aacacgcgct gcgccgggcc ggtgtacgag atcggcaggt acagcgtgaa
     2341 cgtgctgccg ctgcccggct tgctgaccag cttgatctcg ccgccgagca ggctggcgag
     2401 ctcgcggctg atcgccaggc ccaggccggt gccgccgtac ttgcggctgg tgccggcgtc
     2461 ggcctgctgg aacgcctcga agatcagctt ctgcttctcg ggcgcgat
//
LOCUS       DASVOR010000284         6019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265491,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000284 DASVOR010000000
VERSION     DASVOR010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265491"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2736
                     /locus_tag="VF516_05975"
     CDS             <1..2736
                     /locus_tag="VF516_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107256.1"
                     /translation="SPSFIEFESGPVRPIAISPDGTKLFAANTPNATLDIFNITASGL
                     TAAGRVPVGLEPVAVAARSNTEVWVVNHLSDSVSVVTLSGTPHVTRTLLVGDEPRDIV
                     FAGTASRAFITTAHRGQQRTDPSIASVSGAGDPQLTTPSVPRADVWVFDPANLGNTLG
                     GTPVKIMSFFTDTPRALAVSPDHNTVYVAGFHSGNQTSIVNEDFICTGFNPTTPCTNS
                     DGTTSPGGNLGPKTDASGEQAPEVAMIVKFNNTTGHWVDPAGRVWDHTFRFSLPDRDV
                     FSVNANTLSQTAAFSHVGTTLFNMVTNPVSGELYVSNTESQNLTRFEGPGIFGKSTVQ
                     GHLAEVRVTVISGGSVTPIHLNKHIDYTKLAGQPGFDPTAAASSLSTPTEMVVSSDGK
                     TLFLAAFGSSKIGVFDTTALRNNTFDPRQISASYIPVSGGGPSGLVLDEANHRIFVAT
                     RFDNSIKVIDTLSKAQIQSVSMPNPEPASVVQGRPMLYDATHNSGNGEAACASCHTFG
                     DLDDLAWDLGNPDDVVKSNPITINLGSQLIINLNPFGFRGQFNGTGQVGVFHPMKGPM
                     PTQTLRGLVNAGAMHWRGDRANGQFGTNATDASLNFNNFIVAFTGLIGAAQQPSTADM
                     QRFTNFQLQVTEPPNPVRALDNSLNAAQQAGANFFGGTRAADGVNLGFSIGMPTSFTC
                     NGCHELDAAEGEFGTSKNASFEGITSQTFKIPHLRNMYTKVGMFGNAAVPGFDAADSG
                     QVGDQIRGFGFTNEGASPTIFHFVTAKVFHPQINSGFPLINPDTTRRQVEQFLLAFDS
                     DLAPITGQQVTLTSSNGSAVGPRITLMEQRANASFTSKVLGGTVKEIELVARVVQNGA
                     SKGFLFNPGNSTFVPSGGGTALTDAQIRALASTAGQEVTFTAVPTGSGARLAGTL"
     gene            2901..3041
                     /locus_tag="VF516_05980"
     CDS             2901..3041
                     /locus_tag="VF516_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107257.1"
                     /translation="MKKTKQTKLRLVRDAVRALQTHELQQVEGANARGDWTGDSKNVC
                     CA"
     gene            3046..3216
                     /locus_tag="VF516_05985"
     CDS             3046..3216
                     /locus_tag="VF516_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107258.1"
                     /translation="MKKHHKTRLRLERDTLRTLHAHELRQVDGGGPIPVSIFDKCPPP
                     PLTGDSKIECCA"
     gene            complement(3311..3589)
                     /locus_tag="VF516_05990"
     CDS             complement(3311..3589)
                     /locus_tag="VF516_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107259.1"
                     /translation="MALDVDIDDDVDLDSSVELDGSEAIRKRLTPPSTSTSSLPSRSV
                     EARAQDRSTSNVDGGLNVVRRRTSPSTSTLWVNHQVDVDDHERHLEDL"
     gene            complement(3876..5456)
                     /locus_tag="VF516_05995"
     CDS             complement(3876..5456)
                     /locus_tag="VF516_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HEX8107260.1"
                     /translation="MVASSQRPATEVGLSILRAGGNAADAAVAVAAALNVTEPICTGI
                     GGDCFALYYDAKTRAITALNGSGRAPARLTIDRVQAMMREHQLAEWPPYHALTITVPG
                     ACAGWFDLIARHGSLPMSTILAPAIELAENGFPVGEICAYYWQWGLDRQGRFTDMSQL
                     MIDGRAPHAGERFHNPNLARTLRAIAEGGPDAFYRGEIAQATVRAIEAAGGVMAMSDL
                     AVHRSTWDEPISTTYRGVRVWECPPNGQGITALMALNLLEGWELRGQDPLGPDRMHLV
                     AEALRVAFADTRWYVADPRVTQVPVAELLSKDYAARRRGLIDPARAIADVRHGAPVAG
                     SDTVYFCVVDAAGNACSCSNSNYLGFGTGIMPAGWGFTLHNRGHNFRLDPKHPNALVP
                     GKRPYHTIIPGMMTYDDGSLYAPFGVMGGFMQPQGHVQVVVGLIDDGVAPQAALDRPR
                     LCLEPVDGAVVVALEDGVPATTAEALRARGHHIHPGISGFDRALFGRGQVIRRERDGR
                     LVGGCDPRSDGCARSTDD"
     gene            5615..>6019
                     /locus_tag="VF516_06000"
     CDS             5615..>6019
                     /locus_tag="VF516_06000"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX8107261.1"
                     /translation="MVTLLPEPPRPRPRLTDLAELAEPADPVADRPHGNAAEVVTADG
                     RSLPLVSATLRGTARGGIARLVLEQRFDNRHAETLHVTYRMPLPPDGAVSGYAFAIGD
                     RTIVGRVVRTQEARERFEEAVAAGHTAALLEQE"
BASE COUNT          996 a         2199 c         1965 g          859 t
ORIGIN      
        1 tcgccgtcgt tcatcgagtt cgagagcggc ccggtccggc cgatcgcgat ctcgcccgac
       61 ggcacgaagc tgttcgcggc caacacgccg aacgccacac tcgacatctt caacatcacc
      121 gcctccgggc tgacggccgc cgggcgcgtc ccggtcggcc tcgagccggt cgcggtcgcc
      181 gcgcgcagca acaccgaggt ctgggtggtc aaccacctgt cggacagcgt cagcgtggtg
      241 accctgagcg gcacgccgca cgtcacccgc accctgctgg tcggcgacga gccgcgcgac
      301 atcgtgttcg ccggcacggc gagccgcgcg ttcatcacca cggcgcaccg cggccagcag
      361 cgcaccgatc cgtcgatcgc cagcgtctcc ggcgccggcg acccgcagct gaccacgccg
      421 tcggtgccgc gcgccgacgt ctgggtgttc gaccccgcga acctcggcaa cacgctgggc
      481 ggcacgccgg tgaagatcat gagcttcttc accgacacgc cgcgggcact ggcggtcagc
      541 ccggaccaca acaccgtcta tgtcgccggg ttccactccg gcaaccagac ctcgatcgtc
      601 aacgaggact tcatctgcac cggcttcaat ccgaccacgc cgtgcaccaa ctccgacggc
      661 acgacctcgc cgggcggcaa cctcggcccc aagaccgacg cgtcgggcga gcaggcgccc
      721 gaggtcgcga tgatcgtcaa gttcaacaac acgaccggcc actgggtgga cccggccggc
      781 cgggtctggg accacacgtt ccggttcagc ctgcccgacc gcgacgtgtt ctcggtcaac
      841 gccaacacgc tgagccagac cgcggcgttc tcgcacgtcg gcaccacgct gttcaacatg
      901 gtcaccaacc cggtgagcgg tgagctctac gtgtcgaaca ccgagtcgca gaacctgacc
      961 cgcttcgagg gccccggcat cttcggcaag tccaccgtgc agggccacct cgccgaggtc
     1021 cgggtcacgg tgatctccgg cggctcggtc accccgatcc acctcaacaa gcacatcgac
     1081 tacaccaagc tcgccggcca gccgggcttc gatccgaccg cggcggcgag cagcctgtcg
     1141 accccgaccg agatggtggt gtcgagcgac ggcaagacgc tgttcctcgc cgcgttcggc
     1201 tcgagcaaga tcggcgtgtt cgacaccacg gcgctgcgca acaacacgtt cgacccgcgc
     1261 cagatcagcg ccagctacat cccggtgtcg ggcggcggcc cgagcggcct cgtgctcgac
     1321 gaggccaacc accggatctt cgtcgcgacc cggttcgaca actcgatcaa ggtgatcgac
     1381 accctcagca aggcccagat ccagtcggtg tcgatgccca accccgagcc cgcctcggtg
     1441 gtccagggcc ggccgatgct gtacgacgcg acccacaact cgggcaacgg cgaggccgcg
     1501 tgcgcgagct gccacacctt cggcgacctc gatgacctgg cgtgggacct cggcaacccg
     1561 gacgacgtcg tcaagtccaa tccgatcacg atcaacctcg gctcgcagct gatcatcaac
     1621 ttgaacccgt tcgggttccg cggccagttc aacggcaccg gccaggtcgg cgtgttccac
     1681 ccgatgaagg gaccgatgcc cacccagacc ctgcgcggcc tggtcaacgc cggcgcgatg
     1741 cactggcgcg gcgaccgcgc caacggccag ttcggcacca acgcgaccga cgccagcctg
     1801 aacttcaaca acttcatcgt cgcgttcacc ggcctgatcg gcgccgcgca gcaaccgtcg
     1861 accgccgaca tgcagcggtt caccaacttc cagctccagg tcaccgagcc gcccaacccg
     1921 gtccgcgcgc tcgacaactc gctcaacgcg gcgcagcagg ccggcgcgaa cttcttcggc
     1981 ggcacccggg cggccgacgg tgtcaacctc ggcttcagca tcggcatgcc gacctcgttc
     2041 acctgcaacg gctgccacga gctcgatgcg gccgagggcg agttcggcac cagcaagaac
     2101 gcgtcgttcg aggggatcac gtcgcagacc ttcaagatcc cccacctgcg caacatgtac
     2161 acgaaggtgg gcatgttcgg aaacgcggcg gtcccggggt tcgacgcggc cgacagcggc
     2221 caggtcggtg atcagatccg cggcttcggg ttcaccaacg agggcgcgtc gccgacgatc
     2281 ttccacttcg tgaccgcgaa ggtcttccac ccgcagatca actccggatt cccgctgatc
     2341 aaccccgaca cgacgcggcg ccaggtcgag cagttcctgc tcgcgttcga cagcgatctc
     2401 gcgccgatca ccggtcagca ggtcacgctg accagcagca acggcagcgc ggtcgggccg
     2461 cggatcaccc tgatggagca gcgcgccaac gcgtcgttca cgtcgaaggt gctcggcggc
     2521 acggtcaagg agatcgagct ggtcgcccgc gtggtccaga acggcgcgtc caagggcttc
     2581 ctgttcaacc cgggcaacag cacgttcgtg ccctcgggcg gtggcacggc gctgaccgac
     2641 gcgcagatcc gcgcgctggc cagcaccgcg ggtcaggaag tgaccttcac cgcggtgccg
     2701 accggctcgg gcgcccgcct cgccggtacg ctgtagtctc gactccagag gtgccctgac
     2761 tgggggcacc cgcactctgc cgtaaaatcc ggcacaccgg ctcccgtgtc gcacgtcgac
     2821 acgggagctt ttgcgttgtt accgtctcgc cgtcgcgatc gaccgaacat ccccatccac
     2881 agcgacggag aaacccagcc atgaagaaga ccaagcagac caagttgcgc ctggtgcgcg
     2941 acgccgtgcg cgcactgcag acccacgagc tccagcaggt cgagggcgcc aacgcccgcg
     3001 gagactggac cggcgactcc aagaacgtgt gctgcgcgtg acgccatgaa gaagcaccac
     3061 aagaccaggc tgcgcctcga gcgcgacacc ctgcgcacgc tgcacgccca cgaactccgc
     3121 caggtcgacg gcggcggccc gatcccggtg agtatcttcg acaagtgccc gccgcctccg
     3181 ctcaccggag actcgaagat cgagtgctgc gcgtaacgcg ctgacccgcg ccggcgcgct
     3241 caagatgtgt cgatccaagc gttcgatctg ggtccccacc ccgaggttga cctcgtcgtc
     3301 tgatgattcg tcacaagtcc tcgagatgac gttcgtgatc gtcgacgtcg acctgatggt
     3361 tgacccagag cgttgaggtc gacggcgacg tacggcgacg tacgacgttg agcccaccat
     3421 cgacgttgga cgtcgaccga tcctgtgcgc gagcctcgac ggagcgcgac ggcagggacg
     3481 aggtcgaggt cgagggtggg gtcagacgtt tacgtatcgc ttcgctgccg tcaagctcaa
     3541 cgctggagtc aaggtcgacg tcatcgtcga tgtcgacgtc gagcgccacg ggaaccaccc
     3601 cgaacgcgca acttgtgatg aatcatcaga cctcgtcgtt gactccatcg tgggccttga
     3661 cggcggcgta gggcgtcgac cccaccgtcg acctcgacct tgcccatatc ccgtcattct
     3721 cgaggacgat tcctcgcaaa ctgacccggg gttcacgtcc aaggtcgacg acgggctcaa
     3781 cgactacgtc gccctcaagg tcaacgccct gcggcaacgt cgagatcaac ctcaacggcc
     3841 tcggactcag cgcgagctca attttctaca catcttcagt catcggtgct gcgtgcgcag
     3901 ccgtcggaac gcggatcgca gccgccgacc agccggccgt cgcgctcgcg ccggatgacc
     3961 tggccgcgcc cgaacagcgc gcgatcgaac cccgagatcc cggggtggat gtggtggccg
     4021 cgcgcgcgca gcgcctcggc ggtggtcgcc ggcacgccgt cctccagcgc gaccacgacc
     4081 gcgccgtcga ccggctccag gcacagccgc ggccggtcga gcgcggcctg cggcgcgacg
     4141 ccgtcgtcga tcagcccgac cacgacctgg acgtgcccct gcggctgcat gaagccgccc
     4201 atcacgccga acggggcgta cagcgagccg tcgtcgtacg tcatcatgcc cgggatgatc
     4261 gtgtggtacg gccgcttgcc gggcacgagc gcgttggggt gcttcggatc gagccggaag
     4321 ttgtggccgc ggttgtgcag cgtgaagccc cagcccgccg gcatgatccc ggtgccgaac
     4381 ccgaggtagt tgctgttgct gcacgagcac gcgttgccgg cggcgtcgac cacgcagaag
     4441 tacacggtgt cgctgccggc gaccggcgca ccgtggcgga cgtcggcgat cgcgcgcgcc
     4501 gggtcgatca gcccgcggcg ccgcgcggcg tagtccttgg acaggagctc ggcgaccggc
     4561 acctgggtca cccgcggatc ggcgacgtac cagcgggtgt cggcgaacgc cacgcgcagc
     4621 gcctcggcga ccaggtgcat gcggtcgggg ccgagcgggt cctggccgcg cagctcccag
     4681 ccctcgagca ggttgagcgc catcagcgcg gtgatgccct ggccgttggg cgggcactcc
     4741 cagacccgca cgccgcggta cgtcgtcgag atcggctcgt cccaggtcga gcgatgcacc
     4801 gcgaggtccg acatcgccat cacgccgccg gccgcctcga tcgcgcggac ggtcgcctga
     4861 gcgatctcgc cgcggtagaa cgcgtcgggg ccgccctcgg cgatcgcgcg cagcgtgcgc
     4921 gccaggttcg ggttgtggaa tcgctcgccg gcgtgcggcg cgcggccgtc gatcatcagc
     4981 tggctcatgt cggtgaaccg gccctggcga tcgagccccc actgccagta gtacgcgcag
     5041 atctcgccga ccgggaagcc gttctcggcg agctcgatcg ccggcgcgag gatggtcgac
     5101 atcggcagcg agccatggcg cgcgatcagg tcgaaccagc cggcgcaggc cccgggcacg
     5161 gtgatcgtga gcgcgtggta cggcggccac tcggcgagct ggtgctcgcg catcatcgcc
     5221 tggacccgat cgatggtcag ccgggcgggt gcgcggcccg agccgttgag cgcggtgatc
     5281 gcgcgggtct tcgcgtcgta gtacagcgcg aagcagtccc cgccgatccc ggtgcagatc
     5341 ggctcggtga cgttgagcgc cgcggcgacc gcgaccgccg cgtcggccgc gttgccgccg
     5401 gcgcgcagga tcgagagccc gacctcggtc gccgggcgct gactcgaagc gaccatgcct
     5461 ggcatcgccg ggtaccctac cccagagcct cgtgcgcgcg ccatcggcgc aactgccgga
     5521 atttgcatgt cggtagaacc tgcgtttttg gctgtcacca ggaccacggc tcccgcgttg
     5581 gtcgggcaga agcgccccga aaggaacgac tacgatggtg acgctgctgc ccgaaccacc
     5641 gcgccccagg ccccgcttga ccgacctcgc cgagctcgcc gaacccgccg atccggtcgc
     5701 tgaccgcccg cacggcaacg cggccgaggt cgtgaccgcg gacggccggt cgctcccgct
     5761 ggtgtcggcg accttgcgcg gcaccgcgcg cggcggcatc gcgcggctgg tgctggagca
     5821 gcggttcgac aaccgccacg ccgagacgct gcacgtcacc taccgcatgc cgctgccacc
     5881 cgacggcgcg gtcagcggct acgcgttcgc gatcggcgat cgcacgatcg tcggccgggt
     5941 cgtccgcacg caggaagcgc gcgagcggtt cgaggaagcg gtcgccgccg gccacaccgc
     6001 ggcgctgctg gagcaggag
//
LOCUS       DASVOR010000285         3532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_557965,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000285 DASVOR010000000
VERSION     DASVOR010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557965"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VF516_06005"
     CDS             <1..355
                     /locus_tag="VF516_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107262.1"
                     /translation="VPSRIGAPAARIGATAAAPLVPAIQRAAGFDQMVARAAATQRQV
                     ANQFMVDLRRPAASAAVAAPAAAAQPTARGLALDGEPLLDAADRDLGALFGEATETPA
                     DEEAELFFADLSSVT"
     gene            complement(404..766)
                     /locus_tag="VF516_06010"
     CDS             complement(404..766)
                     /locus_tag="VF516_06010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107263.1"
                     /translation="MLNIDVQLVSYDAPKIFFVGAERREVRQAVEITVQTAAPIPARA
                     LSPVLFVGNAEVVEMEALGRNRYKFYALEPARLEQGAAIAFGWPGQAAGSRDDGTALA
                     QRTVTSFRYRLAGGRGVA"
     gene            complement(830..2884)
                     /locus_tag="VF516_06015"
     CDS             complement(830..2884)
                     /locus_tag="VF516_06015"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8107264.1"
                     /translation="MGKRPFGSFERFEASAFRSFGPVSERERRNADLFFADLMGWPAP
                     ARRTRRRRIDGGREAEAVGTLNAARFSGNADLRRVINRTRLLQLATPRMTGDDIRIVQ
                     HALIDLGYTLPVRGVDGIYGPETAAAVRKFQTEQRRLDPAFLIDGIVGDQTLGRMDDI
                     LAILDRPVGARAAAPVDPTAAVIAQPGRLPYDAGTATVLGVTLHVRGTIFYPAQTAGT
                     GTPFSAAVTGKAPIVFLAHGNHATFHNPADRTDEACPGDPGWLSTWPRINNHDGYVYL
                     QEALAQLGFISVSVDCNDTNCSGLSATNIRRRSGLIVESVRHLISLAAAGSGSVLSGR
                     VEFGQTGLLGHSRGAEAVLVVPSDIAAQGLSSVHVGGIISLAPTDTGTASPLPTGLPY
                     MVILPAADGDVRANSGAHFYDRIAPAPFKTQLYVYRTNHNRFNREWVNDDFPSPLISR
                     AAHERLLTVYAAAFFRHALGGVNFLDILLGRTQVAGVSNDDIRISAERSGVVTIDDHE
                     DVPTPPATRTTTNTLGGATTLSGATAVEIPFSQPGVPPDNTFFGDTRGMVASSAASYG
                     TFRSALPAPTNITGREVWIRACEVFNAPAIPPGATQFQIGVEDAAGEVAFVPSGLVAR
                     PFDRTAVDLVTKSMPETFRFAHTSFALARPGVDLTRIRAVVIRLSGIPARKMAFDQLQ
                     LV"
BASE COUNT          538 a         1214 c         1242 g          538 t
ORIGIN      
        1 cgtcccctcg cgcatcggtg cacccgccgc tcgcatcggc gcgaccgccg cggcaccgct
       61 cgttccggcg atccagcggg cggctggttt cgatcagatg gtcgcgcgtg cggcggcgac
      121 ccagcgccag gtcgcgaacc agttcatggt cgacctcagg cgccccgccg cgtcggcggc
      181 ggtggctgct ccggcggctg ccgcgcagcc caccgcgcgc ggcctcgcgc tcgacggcga
      241 accgctgctc gatgcagcgg atcgcgatct cggcgcgctg ttcggcgagg ccacagagac
      301 gcccgccgac gaggaggccg agctgttctt cgccgatctt tcctccgtga cgtgatcccg
      361 atgttgttca gcgtcaccgg gtgagagggc gcatgcgcgc cgctcaggcg accccacggc
      421 cgccggccaa ccggtaccgg aaactggtca cggtccgctg cgcgagcgcc gttccgtcgt
      481 cgcggctccc cgccgcctgg ccgggccaac caaacgcgat cgcggcgccc tgctcgagcc
      541 gcgccggctc gagcgcgtag aacttgtatc gattgcggcc gagcgcctcc atctccacga
      601 cctccgcgtt gcccacgaac agcaccggtg agagggcacg cgccgggatc ggtgccgcgg
      661 tttgcaccgt gatctcgacg gcctgccgga cttcccgtcg ctccgcgccg acgaagaaga
      721 tcttcggtgc gtcgtagctc accagctgaa cgtcgatgtt cagcacgcgg ggcaggtccc
      781 acagcgtgcc ggtcgttctg cgcgtcgtca tcgcttggag ctcctcgggt cacaccaact
      841 gtagctgatc gaacgccatc ttgcgggccg ggatcccgct cagtcgaatg accacggcgc
      901 ggatgcgcgt gaggtcgacg cccggccgcg cgagcgcgaa cgacgtgtgc gcgaaccgga
      961 atgtctccgg catgctcttc gtgaccaggt cgacggccgt ccgatcgaag ggacgagcga
     1021 ccagacccga cggcacgaag gcgacctcgc ccgccgcgtc ttccaccccg atctgaaact
     1081 gcgtcgctcc cggcgggatc gccggcgcat tgaacacctc gcaagcccgg atccagacct
     1141 cgcgcccggt gatgttcgtc ggcgccggca gcgcggaccg aaacgtcccg tacgaggccg
     1201 cgctcgaagc caccatcccg cgcgtgtcgc caaagaacgt gttgtccggc gggacgccag
     1261 gttggctgaa tggaatctcc accgcggtcg cgccggacag cgtggtcgca ccgcccagcg
     1321 tgttcgtcgt cgttcgtgtc gccggcggag tcggcacgtc ttcgtgatcg tcgatcgtga
     1381 ccacgcccga acgctcggcg gagatgcgaa tgtcgtcgtt ggagacgcca gcgacctgcg
     1441 ttcgcccgag gaggatatcg aggaagttca cgccgccgag cgcatgtcga aagaacgctg
     1501 cggcatacac ggtcagcaag cgttcgtgtg ctgcgcgcga gatgagcggc gatggaaaat
     1561 cgtcgttcac ccattcgcgg ttgaaccggt tgtgattcgt gcggtagacg tagagctgcg
     1621 tcttgaacgg ggccggcgcg atgcgatcgt agaagtgcgc gccgctgttc gcccggacat
     1681 cgccgtcggc ggcgggcagg atcaccatgt acggaagccc cgtggggagc ggcgaggccg
     1741 tccccgtgtc cgtcggcgcg agtgagatga taccgcccac gtgaacgctc gatagaccct
     1801 gcgcggcgat gtcgctcggg acgaccagca ccgcttcggc gccgcgcgaa tgaccgagca
     1861 ggccggtctg cccgaactcc acacgacccg agagcaccga tccgctgcct gccgccgcga
     1921 gcgagatcaa atggcgcacc gattcgacga tgagcccgga tcggcgccgg atgttggttg
     1981 cggacaggcc cgaacaattg gtgtcgttgc aatcgacgga gaccgagatg aaaccgagct
     2041 gcgccagcgc ctcctgcagg tacacgtatc cgtcgtggtt gttgatgcgg ggccaggtcg
     2101 acagccagcc ggggtcgccg gggcaggcct cgtcggtgcg gtcggccgga ttgtggaagg
     2161 tggcgtggtt tccgtgcgcg agaaagacga tgggcgcctt gccggtgacg gcggcgctga
     2221 acggcgtgcc tgtgccggcg gtttgcgccg gatagaagat ggttccccgc acgtgcagcg
     2281 tgacaccgag caccgtggcc gtcccggcat cgtaggggag gcggccaggc tgcgcgatga
     2341 ccgcggcggt cggatcgacg ggcgccgcag cgcgcgcacc gaccggtcga tcgaggatcg
     2401 cgaggatgtc gtccatgcgg ccgagcgttt ggtcccccac gatgccatcg atgaggaacg
     2461 cgggatcgag gcggcgctgc tcggtctgga atttccgcac ggccgctgcc gtctcggggc
     2521 cgtagatgcc gtcgaccccg cgcaccggga gcgtgtagcc gagatcgatc agcgcatgct
     2581 gcacgatgcg gatgtcgtcg ccggtcatcc ggggggtggc gagctggagc aggcgcgtgc
     2641 ggttgatgac cctccgcaga tcggcgtttc cgctgaagcg cgctgcgttg agcgtgccca
     2701 ccgcctcggc ttcgcgaccg ccatcgatgc gccggcggcg cgtccgacgc gcgggcgcgg
     2761 gccaccccat caggtcggcg aagaacaggt cggcgttgcg ccgctcgcgc tccgacacgg
     2821 ggccgaagct gcggaaggcg gacgcttcga accgctcgaa cgagccgaat ggtcgcttcc
     2881 ccatgtgacc tccggcgcga actgtacgct gaagcacgtt cggggcgcaa tgcctcgatc
     2941 tcacattccg ggtacaatgg cgcccacccg atgaacaagc gcgcgatgat cccgacgctg
     3001 gtgctcgcag cgcttgcggg ctgcggaacc ggcggcaaga gcccgagccc ttcctcgcaa
     3061 ccgccttcga gtgccagcac ggcgagcccg ccggccggcg atccagcgtg cgagagcgcg
     3121 tgcgtgatca tcgaggcgtg caccggtcgg ccccagacct ggtgtcgccc ggactgcgcg
     3181 acggacatcg ctcctcgggg cgcgggcgca gaatacctga cctgcgtgaa ggcgctctcg
     3241 tgcggtgata tcaaggcatc gctgggaatg aacgaaggtc ccctcgggcg gtgttatcca
     3301 agggcccaca ggtagcgcga tcgaggaccc gccgtacgac ccgagatgcc cacgcgcaac
     3361 ggtcgcagcc ggcgacgtgg tgcacgatcc gcgctaggct cgcgtgcgcc gatgcccgcc
     3421 gcccgccgct tcccgctcgc gctggtgctg gcgatcgtcg cgatctcgac cggcgcgccg
     3481 ttcgcgcgct gggcccagcc ggcgccggcg atcgcgatcg ccgcgctgcg gg
//
LOCUS       DASVOR010000286         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353357,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000286 DASVOR010000000
VERSION     DASVOR010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353357"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(538..768)
                     /locus_tag="VF516_06020"
     CDS             complement(538..768)
                     /locus_tag="VF516_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107265.1"
                     /translation="MSIRAKASSGRIIVDEPTLLPERTTLNLVVDDEGDDLDDNERDA
                     LNAHLLAGWQSAQAGRVRPASELLAELRSKHR"
     gene            complement(843..2579)
                     /locus_tag="VF516_06025"
     CDS             complement(843..2579)
                     /locus_tag="VF516_06025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8/S53 family peptidase"
                     /protein_id="HEX8107266.1"
                     /translation="MARAQGVPPLFNHGLRWIGSAATCQAPGGWTASRLFRSGQLGAA
                     LDRLCVYRWNQAGTLPTQSDIADLFTVSGSDDLTEDVPVVYQSAAFSPPEIAFLTGLR
                     TALSAQVGNATLLPHAPGPQTPAARVVVVDTAPDAPHGQIHPGVSRHGDTLAHLIEDL
                     VCLPATPTGWICAAEVTTALALDGGVGTLSDLASAIERAVVLWQSGRRDAPATTPAHL
                     ILNLSLGWENQPGIADCSTQLSESAAPPVRAVAGILQYAAAQGALIIAAAGNDSGGPK
                     PRTGLVCPGRYQAVPRDADPKQSLVVAVSGVDYQDRPLATARPLGITGIAGLGFGGVA
                     WNPADPVPPPLTGSSVSTAVVSAVSALVWSQQATWNAAQITRAVYNGGVAVGPANECP
                     LAIPGCASHRASLCGALHAAGIAVPCAPAAPYPGSNPALPTEIAEIDLAFGSLPPTAG
                     TAAPTAAPLPRDTLPGLQIQPWIYPMPIAETCPVCVVENNQLLMPALTRPIDDTVLVV
                     RYADGTEQEVALAPPLPAPTTLASGIPYWFALPGPPSSTSGPIVAAYLTAYAIPPALP
                     PYSISEPLFVEP"
BASE COUNT          429 a          909 c         1134 g          379 t
ORIGIN      
        1 cggagtcggg gcaggcgccg cggcctgcgg cgagggcggg gtcgtgggcg gcgcggtggc
       61 cgcaccgcag ccacaggcga gcaggacaat gcaaaggcaa cgcatccccg gaagacgcac
      121 cagggcgcgc ggcggttgac agcggaccgg cagcgccatt gcttgcgttg cattcacgag
      181 ccgggcttct tggccgagct gtcggaggca gcgagcactc ggcgggtgct cgctgctcga
      241 tctccgaatg gtggcgtcgt ccctcgggtt gcaccccaga tggcaatgac caccagatcg
      301 tcggtgtata cgaagtactg gtgatagcgc gtcgaccgaa gcaggtatcg ccggagcccc
      361 ggaatggcag agtgcgagaa ccggactccg acgccggctt ccattccgag gagtgcgatt
      421 gcgtcggcaa gctcctcgac gaagaggttc ggggctgcgc cgcgatgctg gatccacccc
      481 tcgtcaatcg ttgcgcgtcg gcgtctgctt cgggcgtcgt gacgacgcgc cggtgcttca
      541 tcggtgcttg ctgcggagct cggccagcag ctcgctggca ggacgaaccc ggccggcctg
      601 ggcgctctgc cagccagcga ggaggtgcgc gttgagtgcg tcgcgctcgt tgtcgtcgag
      661 atcgtcgcct tcgtcatcga cgacgaggtt caacgtggtg cgctcgggca ggagcgtagg
      721 ctcatccacg atgatgcggc cgctcgacgc cttggcgcgg atcgacatgc tgaccagcat
      781 agcatctcgc gtgcgccatg ggcgacccgc cgaggccggg ttgctgcatc ggtcctcact
      841 gactacggct cgacgaacag cggctcgctg atcgagtagg gcggcagcgc cggtgggatc
      901 gcatacgcgg tgagataggc cgcgacgatc ggcccggagg tagagctcgg cggacccggc
      961 agtgcgaacc agtacgggat gccgctcgcg agggtcgtcg gggcgggcag cggcggagcc
     1021 agcgccacct cctgctcggt gccgtcggcg tagcgcacga ccaggacggt atcgtcgatc
     1081 gggcgggtga gcgccggcat gagtagctgg ttgttctcga ccacgcacac cggacaggtc
     1141 tcggcgatcg gcatcgggta gatccacggc tggatctgga ggcccggaag ggtgtcgcgg
     1201 ggcagcggag ctgcggtcgg cgcggcggtg ccggcggtgg gcggcaagct gccgaacgca
     1261 aggtcgatct cggcgatctc ggtgggcaag gccgggttgc tgccggggta cggcgcggcg
     1321 ggggcgcagg gtacggcgat gccggcagcg tggagcgcgc cgcacagcga ggcgcggtgc
     1381 gaggcgcacc cggggatcgc cagcgggcac tcgttggcgg ggccgaccgc gacgccgccg
     1441 ttgtagacgg cgcgggtgat ctgggcggca ttccaggtgg cttgctggga ccacaccagg
     1501 gcgctgacgg cggagaccac ggccgtggac accgacgagc cggtgagcgg tggcgggacc
     1561 ggatcggccg gattccacgc gacaccgccg aagccaagac ccgcgatgcc ggtgatgccg
     1621 agcggccggg cggtggcgag cgggcggtcc tggtagtcga cgccggagac ggccacgacg
     1681 agcgactgct tcggatcggc gtcgcggggc acggcctggt agcggcccgg gcacaccagc
     1741 cccgtccgtg gcttgggacc gcccgagtcg tttcccgcgg cggcgatgat cagcgcgccc
     1801 tgcgccgccg cgtactgcag gatgccggcg accgcgcgca ccggcggtgc ggccgactcg
     1861 gagagctggg tggagcagtc ggcgatgccg ggctggttct cccagcccag gctcaggttg
     1921 aggatcaggt gcgccggggt ggtggccgga gcatcgcggc ggccgctctg ccagagcacc
     1981 acggcgcgct cgatcgcgct ggcgagatcg gagagcgtgc cgacgccgcc atcgagagcg
     2041 agcgcggtgg tgacctcggc ggcgcatatc cagccggtgg gggtggcggg caggcagacc
     2101 agatcttcga tcaggtgtgc gagggtgtct ccgtggcggc tgacgccggg gtggatctgg
     2161 ccgtggggcg cgtcgggcgc cgtgtcgacg acgacgacgc gggccgcggg ggtctgcgga
     2221 ccgggcgcat ggggcagcag cgtggcattg ccgacctgcg ccgacagcgc cgtgcgcagg
     2281 ccggtgagga atgcgatctc cggaggagag aacgcagcac tctggtacac gaccggcacg
     2341 tcctcggtca gatcgtccga tccgctgacc gtgaacaggt cggcgatgtc ggactgggtc
     2401 ggcagggtgc cggcctggtt ccagcggtag acgcagagcc ggtcgagcgc ggcgccgagc
     2461 tgcccggagc ggaacaggcg gctcgccgtc catccgccgg gagcctgaca ggtcgctgcg
     2521 gacccgatcc agcgcaggcc gtggttgaag agcggcggta cgccctgggc gcgagccacg
     2581 cccgccgcac acaggccgag gaccaacaac gcggggccga acagcgcagc gccggaccgg
     2641 tggtgagacc gatggcgagc gcggcgaacg aacgcacgga acatgctatc tcctgccgcc
     2701 aacgaacgcg gcgaatctta gtacgcggcc ggtggccggg attggacagc gcggcgccgg
     2761 acccgcagca cccggacgtg cagcacgccg cgggggcaac gacggccgca cggggcggac
     2821 ccgcggctcg aggccctgcg cgctagcgct c
//
LOCUS       DASVOR010000287         1924 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207221,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000287 DASVOR010000000
VERSION     DASVOR010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207221"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VF516_06030"
     CDS             complement(<1..745)
                     /locus_tag="VF516_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8107267.1"
                     /translation="MIPRLATRGKLVLGTALLFLAVGALHGAPPLVGLAGVIVTLLCA
                     LYLGFYPTAILLRRKKIELSWWVPPGDQPGGALAADRPFQLHLAFRNHGSRKLRILST
                     QILAASPLSIDDRPSATVKRGQQVEVTTMVKPLAAGYQVLHGAALTFGDALGLFDIEA
                     FFPNPIAVKVFPRTLAVRGQPVRAVGGALHEQVGQHHVRRRGLSGELRELREHSHGDP
                     FKFIAWKATARRGRLMVRDLENEIVTTHIA"
     gene            complement(753..1730)
                     /locus_tag="VF516_06035"
     CDS             complement(753..1730)
                     /locus_tag="VF516_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8107268.1"
                     /translation="MPVPALTPDRVSRVNQIARAIADEVGRAFIGNPMITEALLVALM
                     ARGHVLIEGYPGVAKTTLVKAFSGTLGCHFRRIQFTPDLLPSDITGTYILDMRTNTFV
                     LREGPVFTNVLLGDEINRAPAKTQSALLEAMQEHQVTIEGETRSLAEPFIVLATQNPI
                     EQEGTYPLPEAQVDRFLIKLKMGYPAPHDEKRMLGTYDRPPPPVRTVVGPNDVLDMQA
                     LTQEVFVAEELLDYVLGLVAFTRSHAKVYLGASPRAGLALVHASKGLALLRGRDYVLP
                     DDVRHLAPLVLGHRILMSPEAELEGATGTAVITEAIDKVGYKMPKGGMR"
BASE COUNT          277 a          645 c          730 g          272 t
ORIGIN      
        1 tcgcgatgtg ggtcgtgacg atctcgttct cgaggtcgcg caccatcagc cggccgcgcc
       61 gcgcggtcgc cttccacgcg atgaacttga acggatcgcc atggctgtgc tcgcgcagct
      121 cgcgcagctc gccggacagc ccgcgccgcc gcacgtggtg ctggccgacc tgctcgtgca
      181 gcgcgccgcc gaccgcgcgc accggctggc cgcgcaccgc gagcgtccgc gggaagacct
      241 tgaccgcgat cgggttgggg aagaatgcct cgatgtcgaa cagcccgagc gcgtcgccga
      301 acgtcagcgc ggcgccgtgc agcacctggt agccggcggc gagcggcttg accatcgtgg
      361 tgacctcgac ctgctggccg cgcttgaccg tcgcgcttgg ccggtcgtcg atcgagagcg
      421 gtgacgccgc gaggatctgg gtcgacagga tccgcagctt gcggctgccg tggttgcgga
      481 acgcgaggtg gagctggaac ggccggtcgg cggcgagcgc gccgccgggc tgatcgccgg
      541 gcgggaccca ccacgacagc tcgatcttct tgcggcggag caggatcgcg gtcgggtaga
      601 acccgaggta cagcgcgcac aggagggtga cgatcacgcc ggcgagcccg accagcggcg
      661 gcgcgccgtg cagcgccccg accgcgagga acaggagcgc ggtgccgagc acgagcttgc
      721 cgcgggtcgc caggcggggg atcatcgcgg gctcaccgca tgccaccctt gggcatcttg
      781 tagccgacct tgtcgatcgc ctcggtgatc accgcggtgc cggtcgcacc ctcgagctcg
      841 gcctccggcg acatcaggat ccggtggccg agcacgagcg gcgcgaggtg gcgcacgtcg
      901 tcgggcagga cgtagtcgcg gccgcgcagc agcgcgaggc ccttggacgc gtggaccagc
      961 gcgagcccgg cgcgcggcga cgcgccgagg tagaccttgg cgtgcgaccg ggtgaacgcg
     1021 accaggccga gcacgtagtc gagcagctcc tcggcgacga acacctcctg ggtgagcgcc
     1081 tgcatgtcga gcacgtcgtt cgggccgacc acggtgcgga ccggcggcgg cggccggtcg
     1141 taggtgccga gcatgcgctt ctcgtcgtgc ggcgccgggt agcccatctt gagcttgatc
     1201 aggaagcggt cgacctgggc ctcgggcaac gggtaggtgc cctcctgctc gatcgggttc
     1261 tgggtcgcca gcacgatgaa cggctcggcg agcgagcggg tctcgccctc gatcgtgacc
     1321 tggtgctcct gcatcgcctc gagcagcgcg gactgggtct tggccggggc gcggttgatc
     1381 tcgtcgccga gcagcacgtt ggtgaacacc gggccctcgc gcagcacgaa cgtgttggtc
     1441 cgcatgtcga ggatgtaggt gccggtgatg tcggacggca gcaggtcggg cgtgaactgg
     1501 atccgccgga agtggcagcc cagcgtcccg gagaacgcct tgaccagcgt ggtcttggcg
     1561 accccggggt agccctcgat caggacgtgg ccgcgcgcca tcagcgcgac cagcagggcc
     1621 tcggtgatca tcgggttgcc gatgaacgcc cggccgacct cgtcggcgat cgcgcgggcg
     1681 atctggttga cgcgggacac ccggtcgggc gtgagggcgg ggactggcat cggcatcgct
     1741 aggcttccag atccggcaac gcggcgccga gagtacgaca cagctcggcc acgtcccggt
     1801 acagggtgtc gaaatcgcgc cgcggcaagt gggcggcact ccacggtgcg gcgacctggc
     1861 cgcggccggg cagcgcgcgc agccggcgct agaccctggg cacatcgcgc cggtacacgg
     1921 tgat
//
LOCUS       DASVOR010000288         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_324097,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000288 DASVOR010000000
VERSION     DASVOR010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324097"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..406
                     /locus_tag="VF516_06040"
     CDS             206..406
                     /locus_tag="VF516_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107269.1"
                     /translation="MEVDKPMHSTEMPTTTQPIEDAPAEGELAELLGAPSALAETDVS
                     APDDREAHDGAPMLACIPGGAL"
     gene            403..669
                     /locus_tag="VF516_06045"
     CDS             403..669
                     /locus_tag="VF516_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107270.1"
                     /translation="MSRRGAQLTPPADLDDLFCLLRDNLGKAEAFISSAEYQMEQAPS
                     GDNEEDQRRQSNVEHLVEAGKFAVRAATYTTQEIEAALARQRRA"
     gene            666..968
                     /locus_tag="VF516_06050"
     CDS             666..968
                     /locus_tag="VF516_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107271.1"
                     /translation="MTGVAEPLDDLRVALGEVDVLANVTAAAYDSADWMEPDPAVIDS
                     VASLLGLIKKSATAAVAAFHRLHGAIADAAPAPAGERWDTEGRGTAREPSDHGGHR"
     gene            1067..1387
                     /locus_tag="VF516_06055"
     CDS             1067..1387
                     /locus_tag="VF516_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107272.1"
                     /translation="MELIGGPVESVPLDGGVHLWCSQGGLYPGLPLNRCVPRDPQLPP
                     GFKDADPARRAAVMALSAHLQVHGEFVLARGNAEAGLEDLTDDDIKRWTRWFEVDAQV
                     RRRK"
BASE COUNT          234 a          549 c          539 g          216 t
ORIGIN      
        1 cgtcaccagc aagcgaggcc ggcgcccgcg ggctccgacg tgagccagag ttccactcac
       61 gcagtcagtt cggcaggcga ctacgtgtag acgcgggtgc atcccccgcc ggtggtccag
      121 cggaatcgca cggttgtcgt gctcgatgtt cgttacatcg ctcgcgacgt cgaagcgttt
      181 gtctcggcct gcctacgttg ctggcatgga ggtcgacaag cccatgcaca gcaccgagat
      241 gccgacgaca acgcaaccga tagaggacgc gcccgcggag ggcgagctgg ccgagctcct
      301 cggcgccccc agcgcgctcg ccgagactga tgtttccgcg cctgacgatc gcgaggcgca
      361 cgacggcgcg ccgatgctcg cgtgcatccc gggaggtgcc ctgtgagccg ccgcggagcg
      421 cagctgacgc cacccgccga cctcgacgac ctgttctgcc tcttgcgcga caacctcggc
      481 aaggccgagg cgttcatcag cagcgcggag taccagatgg agcaggcccc gagcggcgac
      541 aacgaggagg accagcgccg ccagagcaac gtcgagcacc tcgtcgaggc cggcaagttc
      601 gcggtccgcg cggcgaccta cacaacccag gagatcgagg ccgcgctcgc tcgccagagg
      661 cgcgcatgac cggcgtcgca gagcctctcg acgatctgcg ggtcgcgctc ggcgaggttg
      721 acgtcctcgc gaacgtcacc gcagcggcct acgacagcgc ggactggatg gagcccgatc
      781 cggccgtcat cgatagcgtg gccagcctgc tcggcttgat caagaagtcg gcgaccgctg
      841 ccgtggcggc gtttcatcgg ctgcacggcg ccatcgccga cgcagcgccc gctccggctg
      901 gcgagcgctg ggacaccgag ggccgcggta ccgcccggga gccctcggac cacgggggcc
      961 atcgatgagc cgctcggcac gtgtgctggt gtgcttcctc gggatgccgc cgcaggagct
     1021 ccggatgccc tcggaccctg ggctcccggg gcacctcgga ggctcgatgg aactcatcgg
     1081 tggtccggtg gagagcgtgc ccctcgacgg cggggtgcac ctctggtgca gccagggcgg
     1141 gctgtatccc gggctcccgt tgaatcgctg cgtccctcgg gacccgcagc tgccgcctgg
     1201 gttcaaggac gctgacccgg cccggcgcgc tgcggtgatg gccctctctg cacatctgca
     1261 ggtgcacggc gagttcgtgc tcgcccgggg caacgcggag gcgggcttgg aggatttgac
     1321 cgacgacgac atcaagcgct ggacgcgctg gttcgaggtc gacgcccagg tgcgccgccg
     1381 caagtagact gccgcctacg cccccacctc gacggcctcg gccgggcgcg gcggcgacaa
     1441 cctcggctcg aacctcacca gttcggccac cggcatgacc tcggcgcgcg gcgggccggc
     1501 tgtcggccac caggagcgcg cacctacgtg ccgatgcc
//
LOCUS       DASVOR010000289         5379 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148655,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000289 DASVOR010000000
VERSION     DASVOR010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148655"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            790..1125
                     /locus_tag="VF516_06060"
     CDS             790..1125
                     /locus_tag="VF516_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107273.1"
                     /translation="MKESQRSDPFDFNGTLCVLRDNPGQLTIPRSTLENLLHHAAFAA
                     HLVGHLEDKAQPELIRQIGDRVDTIAHELAELLGAPSALAEPDAAAPDDRKARDGVPM
                     LTSIAGGAP"
     gene            1220..1540
                     /locus_tag="VF516_06065"
     CDS             1220..1540
                     /locus_tag="VF516_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107274.1"
                     /translation="MRVPADQAPLFHIPGPHGGWFASLLYRHRSSVDVPMRELRELIA
                     GSRGEVIVARRLTHEEAGAIESRRVNCDAAVHAGIIATEEERFRRPRRRDKHGRKRSH
                     ADQR"
     gene            1524..2255
                     /locus_tag="VF516_06070"
     CDS             1524..2255
                     /locus_tag="VF516_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107275.1"
                     /translation="MPISDDATLRGFIERLLGWSEERTVELALRSLALALDHRATLVL
                     CGKGDLVPIARALHRRTLGADRPFIVCDPRRRTTTASVRSPGSRASGVAAFEAEIGGS
                     LCVRARRLPDDFQALVARLRSADDVLCAICAGQLADAHTPLILPAPLAVPPLTGRAAE
                     LDRIIASLAYSAIAELAAPTSSFTADDHAWVRDHAAASLTEIEAATLRLVAFYTSRNL
                     SHAAARLGMAPVSLSRWLGRRELPR"
     gene            complement(2180..2911)
                     /locus_tag="VF516_06075"
     CDS             complement(2180..2911)
                     /locus_tag="VF516_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8107276.1"
                     /translation="MNWSDPDEKAEAIPMLLEQLTSLEQWVAERLPEELRRPPLQDHV
                     ATLHQIIEQDLEPDPDDPGGAPSRIRRGVAEDRRVSIEDEEMRHGRKTKSKRFNGYKR
                     HLAIDLDTVLVLAVAVLPANRPEQDAAPSLVEDIEAQGRTIDALYIDRGYINAPIVDE
                     IRARRGLVVCRPWIARNGEHFSKSAFAINMRDRTITCPVQDDPGRDLAPVDLALVVRG
                     HHRGNSRRPSHRLSDTGAIPSRAAA"
     gene            3027..3686
                     /locus_tag="VF516_06080"
     CDS             3027..3686
                     /locus_tag="VF516_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107277.1"
                     /translation="MRQEVDGVLHATGALQRAGVHRDPQGLGQLLGIERLGLTCQLDG
                     ALQESTIEVGLDESLPEREQGTLREQRFRRPQAAEHHLPAQIQERQLDRRCVGGARVR
                     LQEQHHRHERRWVSVLAGAGISIHPFELGLKCLVEQLVALQSKEGVELAVSLEPTKDE
                     LLLAADRLVRAPALDRHRVSAAILRGGSRGSRSGARRRSRTEATSQVINFLRASNRSV
                     L"
     gene            complement(3765..>5379)
                     /locus_tag="VF516_06085"
     CDS             complement(3765..>5379)
                     /locus_tag="VF516_06085"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8107278.1"
                     /translation="AGVATTLISPEHARSIGPGRPLEPATRRTMELFFEADFSSVRVH
                     EGPAPSALGALAFTIGDQLHFAKGRYAPTSPAGAELLGHELAHVVQQRSGRVANPYGR
                     AIAIVQDPALEREAHRLGREVAALITPAAARRSASVQRSTKLVNVSFRPYVPRYPSES
                     SLVARLRGIDSLLRLRNGELTDLILAELEDLTEQITDNAWLEATALILTIRDRWHIDQ
                     FPPPLIPADTPHLVEGGVVLEHSNVIYLPRHLASPLVLAPGSNMIELGAGPMDSACAL
                     WSQCEYTAFTEFRSREQLVHDYGEPFQQNLLTIQSLSQSRHNTCVYGIDATQVGPYRN
                     LPLTRILRFTNPNIGHQDIRVHIESMINQAVDDWPESDLVYTCMGMRFTLSLDRLNTY
                     GTLKSWTGNYITTLVASTTNSISTCRNIKLIYLFLLNAPAKLIPGVGVVQIIVSTSYL
                     TRWPLEAMARRMGYGWHVDDGYELGFENIKTQEDVHVSHKAPKKCLITLTPPWHLGFL
                     TVFSVVDCCADLTVYTTQNPSLVSSVKEL"
BASE COUNT          798 a         1804 c         1850 g          927 t
ORIGIN      
        1 tggccgacgg ttgcgctcgc cggccgcgga ctcgcggcgc gtgccgccgc tgagcggcac
       61 gcggggtgct gcgccaccgc gtgccggctc tgacgaccca tatcgtgggt ggccgacggt
      121 tgcgctcgcg ggccgcgtgc actgcggcgt gacccgctga catcggctcc acatggcgcg
      181 gtgcgatccg cttcatctgg gcgggtgttg ccgccgctgg cggtcgtgct tggcgaccgc
      241 tcgctcaacg tatggacgat ccagtccgac gaactgcgtt cgtatctcgc gcggctcttg
      301 gcccttcgtc acgacgcgac gaagcccggc cggagatcca gacctcgctg ctggcctggg
      361 gcgccctgac cagcgatcgg gacagcggcg accgcgggcg tccggactac gtgctccgcg
      421 gggttcgagg tagcgatcgc ctcgtgcggg ttcgtgtgga tccggccagc acgtgatcgg
      481 cggccttcag ggcaacctgc cgcagccacg tcgcgagttg taggtgctcg cgctccgcgg
      541 cctgttccag cttcgccttc tcgctgtccg tgaaccgcac ccccagcacg tgacgtgctg
      601 cgtcgccagc gcggggcttg cgtcctcgga ccatggcgca cgttcgcata ttcgttgacg
      661 aaaaggcaac gtcgtaacgt tcattactat ttagttgttc tttatacaac ggaattgtcc
      721 gatgctgcgc cggtcagtcc tggaatcact atcgtttatt aatgaacaac gatattagag
      781 gatggccgga tgaaggaatc ccagcgaagc gacccgttcg acttcaacgg cacgctgtgc
      841 gtgctacgtg acaaccccgg gcagctgacc atccccagat ccaccctcga gaacctcctc
      901 caccatgccg ctttcgcggc ccatctcgtc ggtcacctcg aggacaaggc acagcccgag
      961 ctcatccgcc agatcggcga ccgcgtcgac acgatcgccc acgagctggc cgagctcctc
     1021 ggcgccccca gcgcgctcgc cgagcctgat gctgccgcgc ccgacgatcg caaggcgcgc
     1081 gacggtgtgc cgatgctcac gtccatcgcg ggaggtgccc cgtgacccgt cgccgcgatg
     1141 acctcgagat cgacgacacg ctccacgccc tccgtgggag cgcgtcgtga ggccccggcg
     1201 atcgcgggat gtcgtgcgcg tgcgcgtgcc cgccgaccag gcgccgctgt tccacatccc
     1261 cggcccgcac ggcgggtggt ttgcctcgct cctctacagg caccgatcca gcgtcgacgt
     1321 cccgatgcgg gagctccgcg agctgatcgc gggctcgcgc ggcgaggtga tcgtcgcgcg
     1381 gcgacttacc cacgaggagg ccggtgcgat cgagagcagg cgggtgaact gcgacgcggc
     1441 agtgcacgca ggcatcatcg ccacggagga ggagcggttt cggcggcccc ggcgcaggga
     1501 caagcacggg aggaagcgca gccatgccga tcagcgatga cgccacgctg cgtgggttca
     1561 tcgagcgcct gctcgggtgg tccgaggagc gcacggtaga gctcgcgctg cgctcgctcg
     1621 cgctcgcctt ggaccaccgc gcgacactcg tgctgtgcgg caagggcgac ttggtgccga
     1681 tcgcgcgggc gctgcaccgg cgcacgctgg gcgcggacag gccgttcatc gtctgcgatc
     1741 cgcggcgccg tacgacgacc gcgtccgtga gatcgccagg gagtcgtgcg agcggcgttg
     1801 ctgcgttcga ggcggagatc ggcggctcgc tgtgtgtgcg cgcccgccgg ttgccggacg
     1861 acttccaggc gctcgtggcg aggctccgga gcgcggatga cgtgctgtgc gcgatctgcg
     1921 ccggccagct cgccgacgcg cacacaccgc tcatcctgcc ggcgccgctc gccgtgccgc
     1981 cgctcaccgg acgcgcggcc gagctggacc ggatcatcgc gtcgctggcg tactccgcga
     2041 tcgccgagct ggccgcaccg acgtcgagct tcaccgccga tgatcacgcg tgggtgcggg
     2101 accacgctgc ggcctcgctc accgagatcg aggcggccac gctgcggctg gtcgcgttct
     2161 acacgtctcg caacctgtct cacgcggcgg cacggctcgg gatggcgccg gtgtcgctga
     2221 gccggtggct cggccgacgc gagttgcccc gatgatgccc tcggaccacg agagccaggt
     2281 cgacgggagc caggtcgcga cccgggtcat cctgtaccgg gcacgtgatt gttcgatcgc
     2341 gcatgttgat cgcgaaggcg ctcttgctga agtgttctcc gttcctcgcg atccacggcc
     2401 ggcagacgac cagccctcgg cgtgcgcgaa tttcgtcgac gatgggtgcg ttgatgtagc
     2461 cgcggtcgat gtagagcgcg tcgatcgtgc gtccctgcgc ctcgatatcc tcgacgaggg
     2521 agggcgcggc gtcttgttca ggacgattgg caggcagcac cgcgactgcc aagacgagga
     2581 cggtatcgag gtcgatcgcg aggtggcgct tgtagccatt gaagcgcttg gacttggtct
     2641 tgcggccgtg ccgcatctcc tcgtcctcga tcgacacgcg gcgatcctcg gcaacgcctc
     2701 ggcgaatccg cgatggcgct ccgccagggt cgtcgggatc tggctcgaga tcctgctcga
     2761 tgatctgatg cagcgtcgcg acatggtctt gcagcggagg gcggcggagc tcctcgggga
     2821 ggcgctcggc gacccactgt tcgaggctcg tgagttgctc gagcagcatc gggatcgctt
     2881 cggctttctc gtcgggatcg ctccagttca tcgaggccct tcttgacact ggactcgagt
     2941 aacagcggcg cgccggcttc ctggcccacg cgctcctcgg tccaccccag gagcttcgcg
     3001 gcgcatacca cgatcttacg gccggcatgc gccaggaggt tgatggtgtc ctccacgcga
     3061 ccggcgccct ccagcgggct ggagtccatc gcgacccgca gggtcttggg cagcttcttg
     3121 ggatcgaacg ccttggtctc acgtgccagc tcgacggtgc gctccaggag tcgacgatcg
     3181 aggttggcct cgatgagtcg ctcccggaac gcgaacaggg caccctgcga gaacaacggt
     3241 tccgtcgccc ccaggcagcc gagcaccatc tgccagcgca gatccaagag cgacagctcg
     3301 accgccgttg cgtcggaggc gcccgcgtac gcttgcagga gcagcaccat cgccatgagc
     3361 gccggtgggt tagcgtcctt gccggcgccg gtatctcgat acatccgttc gagctcggcc
     3421 tgaaatgcct cgtcgaacag ctggtggcgc tgcagtcgaa ggaaggcgta gagcttgccg
     3481 tttcgcttga gccgacgaag gatgaattgc tcctggcggc tgatcgactc gtcagggctc
     3541 cagcgctcga tcgtcatcgt gtctccgccg caatactccg cggcggctcc agaggatcac
     3601 gatcgggggc tcgccgtcga tcccggaccg aggcgacctc gcaagtgatc aatttccttc
     3661 gagcttcaaa ccgatcggtg ctctagggat ccttggaccc gcgaccagcg ccgccgcaac
     3721 tacctcgagc gcgccctcgc cagcgcggcc tcgagggcga ctgctcagag ctccttcacc
     3781 gagctcacca gggacgggtt ctgggtggtg tacaccgtca ggtccgcgca gcagtccacg
     3841 acgctgaaga ccgtgaggaa gccgagatgc cagggaggcg tcagggtgat caggcacttc
     3901 ttcggcgcct tgtgcgagac gtggacgtcc tcctgggtct tgatgttctc gaagccgagc
     3961 tcgtagccgt cgtcgacgtg ccagccatag cccatcctcc tggccatcgc ctccagcggc
     4021 catcgcgtca ggtagctggt cgacacgatg atctgcacga cccccacgcc cgggatgagc
     4081 ttggccggcg cgttcagcag gaagaggtag atgagcttga tgttccggca ggtgctgatc
     4141 gagttggtcg tgctcgccac gagggtcgtg atgtagttgc cggtccagct cttcagcgtc
     4201 ccgtaggtgt tgagccggtc caggctcagg gtgaagcgca tccccatgca ggtgtacacg
     4261 aggtcggact ccggccagtc gtcgacggcc tggttgatca tgctctcgat gtgcaccctg
     4321 atgtcctggt gcccgatgtt cggattggtg aagcggagga tcctcgtgag cgggaggttt
     4381 cggtaggggc ccacctgcgt cgcgtcgatg ccgtacacgc aggtgttgtg ccggctctgc
     4441 gacaggctct ggatcgtcag caggttctgc tggaacggtt cgccgtagtc gtgcaccagc
     4501 tgctcacgcg agcggaactc ggtgaaggcc gtgtactcgc actgggacca gagcgcgcac
     4561 gccgagtcca tcgggcccgc gcctagctcg atcatgttgg acccaggcgc gaggacgagc
     4621 ggcgacgcca ggtgcctcgg caggtagatg acgttcgagt gctcgagcac gacgccgccc
     4681 tccaccaggt gcggcgtgtc ggccgggatg agcgggggcg ggaactgatc gatgtgccat
     4741 cgatcgcgga tggtgaggat gagcgccgtc gcctcgagcc acgcgttgtc ggtgatctgt
     4801 tcggtcaggt cctccagctc ggccaggatg agatcagtca gctcgccgtt gcggagcctc
     4861 agcaggctgt cgatcccccg cagtcgggcg accagcgagc tctcgctggg gtagcggggg
     4921 acgtacggac ggaagctcac gttcacgagc ttcgtgctgc gctgcacgct cgcgctccgc
     4981 cgcgccgccg ccggcgtgat cagcgcggcg acctcccggc ccaggcggtg ggcctcgcgc
     5041 tccagcgcag gatcctgcac gatcgcgatc gcgcgcccgt agggattggc cacccgcccg
     5101 ctccgctgct ggaccacatg ggcgagctcg tggccgagta gctcggcgcc ggccggcgac
     5161 gtcggagcgt accggccctt cgcgaagtgg agctggtcgc cgatcgtgaa ggccagcgcg
     5221 cccagcgccg acggcgccgg cccctcgtgg acgcggaccg acgagaagtc ggcctcgaag
     5281 aacagctcca tggtgcggcg ggtcgccggc tcgagcggcc gcccggggcc gatcgaccgc
     5341 gcgtgctcgg gcgagatcaa cgtcgtcgcg acgccggcg
//
LOCUS       DASVOR010000290         4194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_90434,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000290 DASVOR010000000
VERSION     DASVOR010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90434"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1957)
                     /locus_tag="VF516_06090"
     CDS             complement(146..1957)
                     /locus_tag="VF516_06090"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX8107279.1"
                     /translation="MIGSECVILSGLANISHRDVMCSWRPAGAVNSGPGRFTATERRD
                     AESDAIGESTRLACSIRNDGLATRTPGGVMSVYVPLIGSKRELLPNSHTAGPVDLSEI
                     VSLTIRVRSVGEPMDLVQEAHELGRMPVAERRYLTHAELAQRHGAAEADLDAIAEFAR
                     RRNLGVVRRSALERQVVVKGPLRDLIAAFHADVGMYHHSTGAYRGRQGEITIPDELHG
                     IVTGVFGFDTRLRHRTHHPAGGRAHGRGGHEGVIATEYAARYRFPTESNGMKLDGAGQ
                     TIAIIELGGGYRESDLQAFFSDAGVAMPHVTAVLIDHADNHPTSPSSADAEVMMDIEI
                     AGVVAPGACIAVYFGPNSDQGFLDTLSAAVHDAQRKPGVISISWGAPESATDQQGVQA
                     FHEICAAAASLGITVCAASGDHGCADRGAGDWDGKVHVDHPACDELVLGCGGTQIDGG
                     VDVAWNDGTPFDGSGGGWATGGGISQVFGVPAYQQGLKLPEALHGGKPGRGVPDIAMS
                     ATNYFLRVDGVEGAAGGTSAVAPLMAGLIARLNQAKHKNLGFINPLLYKNPTMLTDVT
                     KGSNAIEHGPPGYQAADGWDACTGLGTPDGIAMLEKL"
     gene            complement(2082..2237)
                     /locus_tag="VF516_06095"
     CDS             complement(2082..2237)
                     /locus_tag="VF516_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107280.1"
                     /translation="MSYVVVKAHDGVNDEVHLEVGDPDRPLDRHISSAGRRLQDRPGV
                     ERPVTAL"
     gene            complement(2436..3836)
                     /locus_tag="VF516_06100"
     CDS             complement(2436..3836)
                     /locus_tag="VF516_06100"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8107281.1"
                     /translation="MVVEDDPEVARAIARKLGGDGLEVELADDPRPVLARLDAGEADG
                     WDVVLLDVGLPGMSGIDVLRRFREANSLASIVMLTGDNTANTATACMRAGAFYYLTKP
                     FRPYELSTMVESAASYSRLRRELARVRPAIDDASDQLLVGTSPAMRKLRAALDRLAGQ
                     DVSILICGESGTGKELVARALHERGARRKHRFVALNCGAIPDALIDSELFGHARGAFT
                     GASTDRPGVFVEANCGTLFLDEIGDMPTAVQARLLRVLQEGEVRPVGSSGVRTVDVRV
                     IAATHVDLTAAVEHGRFRQDLFYRLNVVVLGVPPLRDRLDDLPILAAHFLRKHGGQAP
                     LSLSPDALDVMTRYSWPGNVRELENALMHAIALRQGEVILPESLPATIAARSNGKITT
                     TIPVTSGDGGGDQLPPLTEAKRRASAAFEKSYLTLAMERAKGSISEAARLAGLDRTNF
                     RRLLQRHGIDASQFKG"
BASE COUNT          577 a         1515 c         1472 g          630 t
ORIGIN      
        1 ggcatggcgc aggggtgcgg gcagatctcg tgtgtcctgg cacgcggcga gccaggcgag
       61 cagagcgacg gcggcggtgg cgagcttcat gccgcgacga tatcgcaccg ccgccgcggt
      121 gatccgggaa ccgccgccgt ccggatcaca gcttctcgag catcgcgatc ccgtccgggg
      181 tccccaggcc ggtgcacgcg tcccagccgt cggcggcctg gtagccgggc ggcccgtgct
      241 cgatcgcgtt gctccccttc gtgacgtcgg tcagcatggt cgggttcttg tagagcagcg
      301 ggttgatgaa gccgaggttc ttgtgcttgg cctggttcag ccgggcgatc aggccggcca
      361 tcagcggcgc caccgcgctg gtgccgccgg cggcgccctc taccccgtcg acgcgcagga
      421 agtagttggt cgcgctcatc gcgatgtcgg ggaccccgcg ccccggcttg ccgccgtgca
      481 gggcctccgg cagcttcagg ccctgctggt acgcgggcac gccgaacacc tggctgatcc
      541 cgccgccggt cgcccagccg ccgccggacc cgtcgaacgg cgtgccgtcg ttccacgcca
      601 cgtcgacgcc gccgtcgatc tgcgtcccgc cgcagccgag cacgagctcg tcgcacgccg
      661 ggtgatcgac gtggaccttg ccgtcccagt cgcccgcgcc gcggtcggcg cagccgtggt
      721 cgccggacgc ggcgcacacc gtgatcccga ggctggcggc cgcggcgcag atctcgtgga
      781 acgcctggac gccctgctgg tcggtcgcgc tctcgggggc accccagctg atcgagatca
      841 ccccgggctt gcgctgcgcg tcgtggaccg cggcgctgag cgtgtcgagg aagccctggt
      901 cgctgttggg cccgaagtac acggcgatgc aggcgcccgg cgcgaccacc ccggcgatct
      961 cgatgtccat catcacctcg gcgtcggcgg agctggggct ggtcggatgg ttgtcggcgt
     1021 gatcgatcag gaccgcggtg acgtgcggca tcgcgacccc ggcatcggag aagaacgcct
     1081 ggaggtcgct ctcgcgatag cctccgccga gctcgatgat cgcgatggtc tggccggcgc
     1141 cgtcgagctt catgccgttg ctctcggtcg ggaaccggta gcgcgccgcg tactcggtcg
     1201 cgatcacccc ctcgtggccc ccgcgaccgt gcgcgcggcc gccggccggg tggtgcgtcc
     1261 ggtggcggag ccgggtatcg aagccgaaca cgccggtcac gatgccgtgg agctcgtcgg
     1321 ggatcgtgat ctcgccctgg cggccgcggt acgcgccggt cgagtggtga tacatgccga
     1381 cgtcggcgtg aaacgccgcg atcaggtcgc gcaggggtcc cttgaccacc acctggcgct
     1441 cgagcgcgct ccggcgcacg acccccaggt tgcggcggcg cgcgaactcc gcgatcgcgt
     1501 cgagatcggc ctccgccgcg ccgtggcgct gggccagctc ggcgtgcgtg aggtagcgcc
     1561 gctcggcgac cggcatccgg ccgagctcgt gggcctcctg caccaggtcc atcggctcac
     1621 cgaccgatcg cacgcggatc gtcaggctca cgatctccga gagatcgacg ggaccggctg
     1681 tgtgcgaatt cggcagtaac tctcgcttgc ttccaatcaa cggaacatac acggacatga
     1741 cgcctccagg tgttcgggtc gctaggccat cattgcgaat cgagcaggcc agtcgcgtcg
     1801 actcgccgat cgcgtcggac tcggcatcgc gccgctccgt cgctgtgaac cggcctggac
     1861 ccgagtttac cgcccctgca ggccgccagc tgcacatcac gtctctgtga ctgatatttg
     1921 ccagccccga cagaatcaca cactcactac caatcatccg atatcgcctt ttgaaccgag
     1981 cgagggcgct cgccggcgcc gcgcgatccc cctccgccgc agctcgcctc gcgcggcggt
     2041 gtgccagggc gcgcacggtc gcgacgccgc cgagagcgag ctcacagcgc tgtcaccggc
     2101 cgctcgacgc ccggacgatc ctgcaggcgc cggcctgcgc tggagatgtg ccgatcaagc
     2161 ggtcgatctg ggtcccccac ctcgaggtgg acctcgtcgt tgactccatc atgggccttg
     2221 acgacgacgt aggacatcgg ccccaccgtc gacctcgacc ttgcccatat cccgtcattc
     2281 tcgaggacga gtcctcgcaa atgacccgtg gttcacgtcc agggtcggcg acgggctcaa
     2341 cgactacgtc gccctcaagg tcaacgccct gcgaccacgt cgaggtcaac ctcgacggcc
     2401 tcggactcag cgcgagctca attttctaca catcttcagc ccttgaactg gctcgcgtcg
     2461 atgccgtggc gctgcagcag gcgccggaag ttggtgcggt cgagcccggc cagccgcgcg
     2521 gcctcggaga tcgagccctt ggcgcgctcc atcgcgaggg tgaggtagct cttctcgaac
     2581 gccgcgctcg cccgccgctt ggcctcggtc agcggcggca gctgatcgcc gccaccgtcc
     2641 ccgctggtca ccgggatggt cgtggtgatc ttgccgttgc tgcgcgccgc gatcgtggcc
     2701 ggcagcgact cgggcaggat cacctcgccc tggcgcagcg cgatcgcgtg catcagcgcg
     2761 ttctccagct cgcgcacgtt gcccggccag ctgtagcggg tcatcacgtc gagcgcgtcg
     2821 ggcgacaggc tgagcggggc ctggccgccg tgcttgcgca ggaagtgcgc cgcgaggatc
     2881 ggaaggtcgt cgagccggtc gcgcagcggc ggcacgccga gcacgacgac gttgagccgg
     2941 tagaacaggt cctggcggaa ccggccgtgc tcgaccgccg cggtgaggtc gacgtgggtc
     3001 gccgcgatca cgcggacgtc gaccgtgcgc accccgctgc tgccgaccgg gcggacctcg
     3061 ccctcctgga gcacgcggag cagccgggcc tggaccgccg tcggcatgtc gccgatctcg
     3121 tcgaggaaca gcgtgccgca gttggcctcg acgaacacgc ccgggcgatc ggtgctcgcg
     3181 ccggtgaacg cgcccctggc gtggccgaac agctcgctgt cgatcagcgc atcggggatc
     3241 gcgccgcagt tgagcgcgac gaaccggtgc ttgcggcgcg cgccgcgctc gtggagcgcg
     3301 cgggcgacca gctccttgcc ggtgccgctc tcgccgcaga tcaggatcga gacgtcctgg
     3361 ccggcgagcc ggtcgagcgc ggcgcgcagc ttgcgcatcg ccggagaggt gccgaccagg
     3421 agctggtccg acgcgtcgtc gatcgccggg cggacccgcg cgagctcgcg gcgcagcctg
     3481 gagtagctcg ccgccgactc gaccatggtc gacagctcgt acggccggaa cggcttggtc
     3541 aggtagtaga acgcaccggc ccgcatgcag gccgtcgcgg tgttcgcggt gttgtcgccg
     3601 gtcagcatga cgatcgaggc cagcgagttg gcctcgcgga accggcgcag cacgtcgatc
     3661 ccgctcatcc ccggcagccc gacgtcgagc agcacgacgt cccagccgtc ggcctcgccg
     3721 gcgtcgagcc gcgccagcac cggccgcgga tcgtcggcga gctcgacctc caggccatcg
     3781 ccgccgagct tgcgcgcgat cgcccgggcg acctccggat catcctcgac caccatcacc
     3841 tttgcccgtg tggcagtgtc gtccattcgg caacgaacag gctcgcagag cccaccggga
     3901 aattcaatcg gctacgcgct tcgctcaccc gcagctgacg cgcgcagcgc gagggcgtcc
     3961 tcggtccgca gggtcaccct gcacgtgccg cactcgccgg cgtcggcgag gtggcgaatg
     4021 gggatccggt ggaccgcacg acagtcggtg cagacgcgcc gcatgcggtg gtggtagcgt
     4081 gcagtgcgtg gcgaagacga agggtgccgc gaccggcgcg cggccgggtg ccatccgcgc
     4141 agccacgggc gggacaccgg accggcgcgc cgcggccgcc aggccagcgc ctgg
//
LOCUS       DASVOR010000291         5891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353403,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000291 DASVOR010000000
VERSION     DASVOR010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5891
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353403"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF516_06105"
     CDS             <1..247
                     /locus_tag="VF516_06105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107282.1"
                     /translation="SLLHCMPVYVGTSRGNWVVDDDEISLLEAAPAQEPPPQQQPTLP
                     RTPPPSQSVQDANADAPLIPQRHGSAARGRASRGGFW"
     gene            complement(275..1522)
                     /locus_tag="VF516_06110"
     CDS             complement(275..1522)
                     /locus_tag="VF516_06110"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX8107283.1"
                     /translation="MSGRAAEVLLDEVEVGELVEDDHGYIEFRISERYRAMTRRPVVG
                     QWFEDHRHGVQRGERPGDLPSFFANMIPEGDLRVRIGSRLGVASDDDFAFLCAVGADL
                     PGAIVVHAQGGERPDYTPPPYEDDANDAGWRFSLAGVQLKFSMKRDGNRLTIPGRDDR
                     GNWIVKFAFDSYPELCANEWVTMQWAQRAGFDVPEIELRALRDLEGVLPEADPATPVF
                     VVRRFDRVSGSDSRESARAKRTRVHQEDFQQVVGRQPSKKYDDITYEGVALLAARIVG
                     EDAYDEVLRRLVFVVASGNNDAHMKNWSILYPDGIEAQLSPLYDQVFTAQWPAFSVQM
                     ALKLGGTKEFAAVTLARFRELARRLGQSPEVAEAIVTEATEQIVAAWTEIREDPTVTD
                     AYRAALRRHWQKVPLLQPYASRL"
     gene            complement(1519..3171)
                     /locus_tag="VF516_06115"
     CDS             complement(1519..3171)
                     /locus_tag="VF516_06115"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX8107284.1"
                     /translation="MTAHSIELVRRPEARTESVEDLVAAVLRGEVRIPVFQRGLEWQT
                     HHVLELFDSIYRGFPIGSLLLRRAPATAGPVQIGPVAVFGTETRNALWVIDGQQRLTS
                     LVAGLGRPQTLPTTPDDPFVVYFDPVEQQFHAPPRSGDLPSSWVPAPQLLDGARLTEW
                     VFAWSHGREDELRRVVFEAGRRLREYRVPLYVIESDDEDAVRAIFHRVNTAGKPLKWT
                     IVYDALYGHKSGPPSTLSALADQLEELGMGALDEETQLLPCLVAMRGLDVTRPLGEHL
                     QRDPTALDGAAAEAVAVLRRVLGFLRVDAEIPHLRLLPSSAPLVVLARFFTLHPEPNE
                     RCTTLLVRWVWRWFLLKDSKDMDERTLKRRGVAGIDDDPEASMQRLLELVHGGPSLNF
                     VVANTFDARAAQSRLALLGMASRKPRRLGLTAMADTSEIDVAELIRQADLAAFRPLLP
                     LSGDFTSGPENRLLLPGSGAAFEELRSFIRTHGVDHPVLTSHLIRPRAAQAILEDDIG
                     IALHDRADAIGGSVDDLEERLAEWDRSDRPSLDYLLAQAAGE"
     gene            complement(3268..3885)
                     /locus_tag="VF516_06120"
     CDS             complement(3268..3885)
                     /locus_tag="VF516_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107285.1"
                     /translation="MLVPLALALVARTIVRAHRRRRARRAPDVIQRLRPDQRLRPDFT
                     DPLQRFDEVPELEVTPLSVDAVSAEDVAAADDLAILEDDLDETADRDTAVIAQVEADE
                     TAVISEAVACDLAEPAHERDAGDLYGAHTPPAQDREHPDNDRAFDTGENWLEALEASA
                     AENGPEPERGLDDIVDDEDVLQPPHPSDTRDRPVADYGSGGRSGL"
     gene            complement(3986..4993)
                     /locus_tag="VF516_06125"
     CDS             complement(3986..4993)
                     /locus_tag="VF516_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8107286.1"
                     /translation="MADSDILSVAPLGFQWPTLDPFLFCVHHDDAYPAGNEQLGPAAS
                     LDGRDLGMDFAGKDGWRMYHGTTVPGVPQHPHRGFETVTIVRRGLIDHSDSLGATARF
                     GRGDVQWLTAGKGISHCEMFPLLDPAGANPVELFQIWLNLPRRDKLVAPYFTMLWDRT
                     IPRIEAAGVRVRAIAGKLGDATPPSPPPSSWASRDEADVAIWTIHLQPGASWTVPPAR
                     PGTNRTLYLFRGNATIAGQAFASNHGVQVRPDVAIPIIAGPEGGNEAELLVLQGTPIG
                     EPVVQHGPFVMNTRQEIQQAFVDYQRTRFGGWPWSSDDPVHPRAEGRFARHADGRIER
                     A"
     gene            complement(5097..5435)
                     /locus_tag="VF516_06130"
     CDS             complement(5097..5435)
                     /locus_tag="VF516_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107287.1"
                     /translation="MEERSACAQLKRRFEAAGFHIKENQRFDQDGVWFEIDGFDAARR
                     VGYEYLTKEAGDGWDVDGNVIAYLAERYDRGDLYVLVIDEADAPDAASLDRAIDAFFG
                     DLKAGGVLPR"
     gene            complement(5484..>5891)
                     /locus_tag="VF516_06135"
     CDS             complement(5484..>5891)
                     /locus_tag="VF516_06135"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX8107288.1"
                     /translation="LALAMVFVAQAAHVEMSLRQQLVAMLFLMLSSKGVAGVRGAAIV
                     VLAGALKAFDLPDGGVTLVFGIDAVMDMGRTCVNVVGNCVATVVVAVWEKAIPPEAPI
                     FGPRAARAAERSEAAAYAIATGEIPVREPTPGD"
BASE COUNT          894 a         2030 c         2066 g          901 t
ORIGIN      
        1 cagcctgctg cactgcatgc cggtgtacgt cggcacctcg cgcggcaact gggtcgtcga
       61 cgacgacgag atctcgctgc tcgaggccgc gcccgcgcag gagccgccgc cacagcagca
      121 gccgacactg cctcggacgc cgccgccatc gcagagcgtg caggacgcca acgccgatgc
      181 gccgttgatc ccccagcgcc acggttcagc ggcacgcggt cgcgcaagcc gtggtggctt
      241 ctggtgaggt gactgccgag cctcactccg ctcctcagag gcgggatgcg tacggttgaa
      301 ggagcgggac cttctgccag tgccgtcgca gcgccgcgcg gtacgcgtcg gtcacggtcg
      361 ggtcctcgcg aatctcggtc caggctgcca cgatctgctc cgtcgcttcg gtcacgatcg
      421 cctcggccac ttcgggtgac tgtcccaggc gtcgggcgag ctcccggaag cgcgcgagcg
      481 tgaccgcggc gaactccttc gtgcctccga gcttgagcgc catctggacc gagaacgcgg
      541 gccactgggc agtgaagacc tgatcgtaca gcgggctcag ctgtgcctcg atcccatccg
      601 ggtagaggat cgaccagttc ttcatgtggg cgtcgttgtt ccccgaggcc acgacgaaca
      661 ccaggcgtcg cagcacctcg tcatacgcat cctcgccaac gatccgcgcg gcgagcaggg
      721 cgactccttc gtaggtgata tcgtcgtact ttttcgacgg ttgccgtccg acgacctgct
      781 ggaagtcctc ctggtggacc cgtgtcctct tggctcgagc tgattcacgg ctgtcgctcc
      841 cggacacgcg atcgaaccgg cgaaccacga acacaggcgt ggccggatcg gcctcgggga
      901 gcactccctc caggtcgcgt agcgcgcgga gttcgatctc cggtacatcg aagcccgcgc
      961 gctgggccca ctgcatcgtg acccactcgt tggcgcacag ctccgggtag ctatcgaagg
     1021 cgaacttgac gatccagttg ccccggtcgt cccgtccagg gatggtcagg cgattcccat
     1081 cgcgcttcat cgagaacttc agctgcaccc cggccagaga gaaccgccag ccagcgtcgt
     1141 tggcgtcgtc ctcgtacggc ggtggcgtgt aatccggacg ttcgccgccc tgtgcgtgca
     1201 cgacgatcgc gcccggcagg tcggcgccga cggcgcacaa aaaggcgaag tcgtcgtcgg
     1261 atgccactcc gaggcggctg ccgatccgga ctcgcagatc gccctcggga atcatgttgg
     1321 caaagaacga cggcaggtcg ccgggccgct ctccgcgctg gacgccgtgg cggtgatctt
     1381 cgaaccactg gccgaccacc ggccggcgag tcatggcacg gtagcgctcc gagatccgga
     1441 actcgatgta tccgtggtcg tcctcgacca gctctccgac ctcgacctcg tcgagcaaaa
     1501 cctccgcggc gcgcccgctc attcgccggc tgcctgggcc aggaggtaat cgagggacgg
     1561 gcgatccgag cgatcccact cggcgaggcg ttcttccagg tcgtcgaccg aaccgccgat
     1621 agcatctgct cggtcatgca gcgctatccc tatgtcgtcc tcgaggattg cttgcgctgc
     1681 acgcggccgg atgaggtgcg acgtcaggac aggatgatcg acaccatgcg tgcggatgaa
     1741 cgatcgcagc tcctcgaagg ctgcaccgct gccgggcagc agcaggcgat tttccgggcc
     1801 tgacgtgaag tccccgctca gcggaagcag cggacggaat gccgcgagat cggcctgacg
     1861 gatcagctcg gccacatcga tctcgcttgt atcggccatc gcggtgagtc cgagtcgtct
     1921 cggtttgcga gacgccatgc cgagcagcgc tagccgggac tgtgcagctc gcgcatcgaa
     1981 cgtgttcgcc acgacgaagt tgagagatgg gccgccatgg acgagctcca gtaaacgctg
     2041 catgcttgct tccggatcgt cgtcgatacc tgcgacgccc cggcgcttca aggtgcgctc
     2101 gtccatgtcc ttcgagtcct tcaacaagaa ccagcgccag acccagcgca ccagaagggt
     2161 cgtgcatcgc tcgttgggtt ccggatgcag cgtgaaaaag cgcgcgagca cgacgagcgg
     2221 agccgagctg ggcagcagcc ggagatgcgg gatctctgcg tccacgcgca ggaagccgag
     2281 caccctgcgg agcacggcca ctgcttctgc ggcggcgccg tccagggccg tgggatcgcg
     2341 ctgcaggtgc tctccgagcg ggcgagtgac gtcgagccca cgcatcgcga ccaggcaggg
     2401 cagtagctgg gtctcctcgt cgagcgcgcc catgcccagc tcctcgagtt ggtcggccag
     2461 tgcactcagc gtcgacggtg gcccgctctt gtgaccgtac agcgcgtcgt acacgatggt
     2521 ccacttcagc ggcttgcccg ccgtgttgac gcggtgaaag atcgcgcgca ccgcatcttc
     2581 atcgtcgctc tcgatcacgt agagcgggac gcggtactcg cgcagacgtc ggcccgcctc
     2641 gaacacgacg cggcgcagct cgtcctcgcg gccgtgactc catgcaaaca cccattcggt
     2701 gagcctggca ccgtcgagca gctggggtgc ggggacccag ctactcggga ggtcgccgct
     2761 gcgcggcggt gcatggaact gctgttcgac gggatcgaag tacacgacga acggatcgtc
     2821 cggtgtcgtc ggcagcgtct gcgggcggcc aaggcccgcg accagcgagg tcaggcgttg
     2881 ctgcccatcg atcacccaga gcgcgttcct ggtctcggta ccgaacaccg cgacagggcc
     2941 gatctgcacg ggaccggcag tcgccggcgc gcggcgcagc agcagcgaac cgatgggaaa
     3001 cccccggtag atactgtcga acagctcgag gacatggtgc gtctgccatt cgagcccgcg
     3061 ctggaacacc ggaatgcgaa cctcgccgcg cagcaccgcc gcgacgagat cctcgaccga
     3121 ttcggttcgc gcctcggggc gacgcacgag ctcgatcgag tgggctgtca tggacgcctc
     3181 gatcttacct cggcgcccgg acaagcgcgc tgagatcgcc tgccggtgac agtcggtggc
     3241 cgagacacag ccaggtcggg gcgccgtcta caggccggag cggccgccgg agccgtagtc
     3301 ggcgaccggg cggtcgcgcg tgtccgacgg gtgcggcggc tgcagcacgt cctcgtcgtc
     3361 gacgatgtcg tcgagccctc gctcgggctc ggggccgttc tccgccgcgc tggcctcgag
     3421 cgcctccagc cagttctcgc cggtatcgaa ggctcgatcg ttgtcggggt gttcccgatc
     3481 ctgggccggc ggcgtgtgcg cgccgtacag gtcaccggcg tcacgctcgt gcgccggctc
     3541 cgcaagatcg cacgccaccg cctccgagat caccgcggtc tcgtccgcct ccacctgcgc
     3601 gatcaccgcg gtgtcgcggt cggcggtctc gtcgaggtcg tcctcgagga tcgcgaggtc
     3661 gtcggccgcg gcgacgtcct ccgccgacac ggcgtcgacg gacagcggcg tgacctcgag
     3721 ctcggggacc tcgtcgaacc gctgcagcgg atcggtgaaa tcgggccgga ggcgctgatc
     3781 gggccggagg cgctggatga cgtcgggggc gcgtctcgcc cgccggcggc ggtgggcacg
     3841 gacgatggtg cgcgcgacga gggcgagcgc aaggggtacg agcacgtggc gtggtcgcat
     3901 ggcggatcga ggagcacgcg gcgtgccagg gggcgcggct ccggcagtct ccgagggcgc
     3961 cggcgcgctg cgccttgccg tgaggtcagg cgcgctcgat gcggccgtcg gcgtggcggg
     4021 cgaaccggcc ctcggcgcgc gggtgcaccg ggtcgtcgga cgaccatggc cagccgccga
     4081 agcgggtgcg ctggtagtcg acgaacgcct gctggatctc ctggcgcgtg ttcatcacga
     4141 acgggccgtg ctggacgacc ggctcgccga tcggcgtgcc ctgcagcacg agcagctcgg
     4201 cctcgttgcc gccctcgggg ccggcgatga tcgggatcgc gacgtcgggg cggacctgga
     4261 cgccgtggtt ggaggcgaac gcctggccgg cgatcgtcgc gttgccgcgg aacaggtaca
     4321 gcgtgcggtt ggtgccgggg cgcgccggcg gcaccgtcca cgacgcgccg ggctggaggt
     4381 ggatcgtcca gatcgcgacg tcggcctcgt cgcgcgacgc ccacgagctg ggcggcggtg
     4441 acggcggggt cgcgtcgccc agcttgccgg cgatcgcgcg gacccgcacg cccgcggcct
     4501 cgatccgggg gatcgtccgg tcccacagca tggtgaagta cggcgcgacc agcttgtcgc
     4561 ggcgcggcag gttgagccag atctggaaca gctcgaccgg gttggcgccg gcgggatcga
     4621 gcagcgggaa catctcgcag tgcgagatgc ccttgccggc ggtcagccac tggacgtcgc
     4681 cgcggccgaa ccgcgcggtc gcgcccagcg agtcggagtg atcgatcagg ccgcggcgga
     4741 cgatcgtgac ggtctcgaag ccgcggtggg ggtgctgcgg gacgcccggg acggtggtgc
     4801 cgtggtacat ccgccagccg tccttgccgg cgaagtccat gccgaggtcg cggccgtcga
     4861 gcgacgccgc cggtccgagt tgctcgttgc cggccggata cgcgtcgtcg tggtggacgc
     4921 agaacaggaa cgggtcgagc gtcggccact gaaagccgag cggcgcgacc gagaggatgt
     4981 cggaatcggc catggctggc agcgtgggca tcgatccggg ctgccgcaat cgcgccatgt
     5041 ggcgaggact gttccgccgc accgtgcgcc ggtgacagcg accacggccg ccccggctac
     5101 ctggggagga cgccgccggc cttgagatcg ccgaagaatg cgtcgatcgc ccggtcgagc
     5161 gatgcggcgt ccggcgcgtc ggcctcgtcg atgaccagca cgtagagatc gccgcgatcg
     5221 tagcgctccg cgagataggc gatcacgttg ccgtcgacgt cccagccgtc gcccgcctcc
     5281 ttggtgaggt actcgtagcc gacgcggcgc gcggcgtcga acccgtcgat ctcgaaccac
     5341 acgccgtcct ggtcgaaccg ctggttctcc ttgatgtgaa acccggccgc ttcgaaccgc
     5401 cgcttcagct gtgcacatgc ggagcgctct tccacgcctg catcgtacgc cagcgcggcg
     5461 gcgactgcac gcccggccgc ccgtcagtcg cccggcgtgg gctccctcac cggaatctcg
     5521 ccggtcgcga tcgcgtaggc cgcggcctcc gaccgctcgg ctgcgcgcgc ggcgcgtggc
     5581 ccgaagatcg gcgcctcggg cgggatcgcc ttctcccaca ccgcgaccac gacggtcgcg
     5641 acgcagttac cgacgacgtt gacgcaggtc cgccccatgt ccatcaccgc gtcgatgccg
     5701 aacaccagcg tgacgccgcc gtcgggcagg tcgaacgcct tgagcgcacc ggcgagcacg
     5761 acgatcgcgg cgccccgcac gccggccacg cccttgctcg acagcatgag gaacagcatc
     5821 gcgacgagct gctggcgcaa cgacatctcg acgtgggcgg cctgggcgac gaacaccatc
     5881 gcgagcgcca g
//
LOCUS       DASVOR010000292         5623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_558044,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000292 DASVOR010000000
VERSION     DASVOR010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558044"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1468
                     /locus_tag="VF516_06140"
     CDS             <1..1468
                     /locus_tag="VF516_06140"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8107289.1"
                     /translation="MPAYPARHAMYDRRMVKRLSPSDAFFLYHESREQMAHDASLMPF
                     TPAPDSPPDLLRGLMDELRGAPPVCSPWNLRLRTPDLRWNPLHSWVEEPGVDLEYHVR
                     RSALPSPGDEHELGILMSRLHECRVDLHRPPWEAHCIEGLERGRFAWYIKTHHSLVDG
                     YSAIQAFTHGLSSDPDERDRPLFFSTPPRARSKPPPASAAGEAGEPEAGSRGIHLREL
                     LGAVREQYGASKAVIRALVDIVQASRAGDHDLVSPLEAPRCVLNARISKSRRFATQSL
                     SIARMRAIARAANGTLNDVALALWAASLRTYLLEQGALPTAPLVAMVPVSIRAKDELG
                     GGNSVGAILATLATDVADPAERLARIVASTTRAKQQLRGMSKAAILQYSALLAAPSML
                     QAIPGAAGHLRPSFNVVISNIPGPDRPLYFRGARLEAWYPMPSPFHGQALMICFISYA
                     GQVCFGLTGCRDTVPHLQRLAVYCGEALSELEHAVLHA"
     gene            complement(1862..2623)
                     /locus_tag="VF516_06145"
     CDS             complement(1862..2623)
                     /locus_tag="VF516_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107290.1"
                     /translation="MNAELEQYEIMIAAERQRFADRMTPFHNETRPTVADLFLIHFCS
                     RGVGMTEPVEDWIRRAGENTCKIGYEELGSGLIRHAEHEAGHHELMIADTRSLVTRWN
                     ASHDTKLDPEAFLSQPLSRNVVRYRELHEEYIRGESPYCQIAIEYEIERISVTYGALF
                     FARIIQLVGSDVLGCLSFIHEHVALDVGHTNYNMRLMERFLEKVPAALEPLVAAGARA
                     LDIYASHLEDCMQFAELMDGQHRMSEVANVAQSLT"
     gene            complement(2613..3551)
                     /locus_tag="VF516_06150"
     CDS             complement(2613..3551)
                     /locus_tag="VF516_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107291.1"
                     /translation="MPDSSDIEALTPRQKRELLARLLNSKPAPEYPDLNLAPPSNLTF
                     KVAETRQELESAFRILHDAYVSMGFMRPSPSGMRITIFNALPGTHTMIALWDGEAIGT
                     MSIIQDSPMGFPIERVYRIDRLRAENVPLGEISAFAVKKTYRGDWNMILFPLIKFAYE
                     YCSRHLGIERILMLTHPKRSEFHERILFCRRISAQVIDECEYVNGAPAVGFYLDIREF
                     LEMCHARHGKEVSAHVATSLTNFVFPARTEVPSPPSMDPEAIDYFFNQKTDLLSKLSD
                     GDRGILMSLYKDDRYRDILSRAQDKQKHSRQGQDER"
     gene            complement(3535..>5623)
                     /locus_tag="VF516_06155"
     CDS             complement(3535..>5623)
                     /locus_tag="VF516_06155"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8107292.1"
                     /translation="GYSLGMLGLECSGRITAVGDGVHGLREGDAVLASGSGCLASQLT
                     VDARFVVRNPGHLSFEECATIPIAFVTAYYSLHKLARIQRGERVLIHAGTGGVGLAAI
                     QLAQRAGAEVFATVGNPLKRDLLTALGVRHVMNSRTVAFADEVLEITGGEGVDIVLNS
                     LSGEAIPKGLSILRHGGRFVELGKRDIYENSRISLRPFGDNVSFFSVDLDRVGARHAE
                     TMRAMFGELMAAFENKELHPLPYRLFSLDRTAAAFRHLAQAQHVGKVVISFEDTGDAL
                     VARPTEPFRARADGSYLITGGLGGLGLAVAHYLVDRGARHLVLAGRRGQATPDIADDM
                     ESLERKGARVTLAALDVTSERQVGDLLASIRTSGAPLCGIIHAAMVPDDTLVQNMTQD
                     QMWRVLLPKALGAWHLHRLTKEDPLDLFVMFSSFTSLIGNPGQANYVAGNAFLDELAH
                     YRRFRGLPALTINWGAVTGTGYVARNRVVEEHVERLGMKGQPVQQILAAFGQLLESGA
                     VQAGVALIDWRRVRQQLYGSRIPPRLSTLMTAAGADESSTDHVALHAILDAAPTERPA
                     LIEDYICSHLARVLGTVASKIEVDRSLVSLGLDSLMAVEIRKRVLTEFGVDIPPMKFM
                     EGISISGAAAFMAERLAAMHPAAANSTRHVPIAAEQPPTEVAVNVDELSDDEVDRRLR
                     TLIDAGARDAGLV"
BASE COUNT         1027 a         1836 c         1737 g         1023 t
ORIGIN      
        1 tatgccagcg tacccggccc gccacgccat gtacgatcgc cgcatggtca agcgcctgtc
       61 gccgagcgac gcgttcttcc tgtaccacga gtcgcgcgag cagatggcgc acgacgcctc
      121 gctgatgccg ttcaccccgg cccccgacag cccgcccgac ctgctccgcg ggctgatgga
      181 cgagctgcgc ggcgcgccgc cggtgtgctc gccgtggaac ctcaggctgc gcacccccga
      241 cctgcggtgg aacccgctgc actcgtgggt cgaggagccc ggggtcgatc tcgagtacca
      301 cgtccggcgc tcggcgctgc cgtcgcccgg cgacgagcac gagctcggca tcctgatgtc
      361 gcggctgcac gaatgccggg tcgacctgca tcgcccgccg tgggaggccc actgcatcga
      421 gggcctcgag cgcggccggt tcgcctggta catcaagacc caccactcgc tggtcgacgg
      481 ctacagcgcg atacaggcct tcacccacgg gctgtcaagc gatcccgacg agcgcgaccg
      541 cccgctgttc ttctcgaccc cgccgcgcgc ccggtcgaag ccgccgccgg cgagcgccgc
      601 cggcgaggcc ggcgagcccg aggccggctc gcgcggcatc cacctccgcg agctgctcgg
      661 cgcggtgcgc gagcagtacg gcgcgtccaa ggcggtgatc cgcgcgctgg tcgacatcgt
      721 ccaggcgtcg cgcgcgggcg accacgacct ggtgtcgccg ctcgaggcgc cgcgctgcgt
      781 gctcaacgcg cggatcagca agagccgccg gttcgcgacc cagagcctgt cgatcgcccg
      841 gatgcgcgcg atcgcgcgcg cggccaacgg cacgctcaac gacgtcgcgc tcgcgctgtg
      901 ggctgcgagc ctgcgcacct acctcctgga gcagggcgcg ctgccgacgg cgccgctggt
      961 cgcgatggtg ccggtctcga tccgcgcgaa ggacgagctc ggcggcggca actcggtcgg
     1021 cgcgatcctc gcgacgctcg cgaccgacgt cgccgacccc gccgagcggc tggcgcggat
     1081 cgtcgcgtcg accacgcgcg ccaagcagca gctgcggggc atgtccaagg cggcgatcct
     1141 gcagtacagc gcgctgctcg ccgcgccgtc gatgctgcag gcgatcccgg gcgccgccgg
     1201 tcatctccgc ccatcgttca acgtggtcat ctccaacatt ccggggccgg atcgcccgct
     1261 gtacttccgc ggcgcgcggc tcgaggcctg gtatccaatg ccgagcccgt tccacggcca
     1321 ggcgctcatg atctgcttca tcagctacgc cggccaggtg tgcttcggcc tcaccggctg
     1381 tcgcgatacc gtgccacact tgcagcgcct cgcggtgtac tgcggcgagg cgctgtccga
     1441 gctcgaacac gcggtgcttc atgcatgatc gccggttcac cgcggcgcgg tcagcgcggt
     1501 gaacacgccg tccgggatca accggtggaa gtgaacgtat aggttgacca gcccaccgaa
     1561 gcgctggacc aacgtgatcg ccccggtccg cggcgctgcg aaactgcgag cacgcgcgac
     1621 acgccgcagc cacgcagcca ccgcgcggac gatgagtcca ccgaccagct ggccacacgg
     1681 aatcgcgggc cacgcgcaga cggaggagct gtgcgacgtc accgtgctgc gccagcgcgc
     1741 ggcgcagatg aagccgtagc gcccccccgc tacgccaata gagcaacgcg aagccattga
     1801 acttggtttc gtgctgttgt ctttcccgaa tccggcgacg ccaccccgtg ggtgacactg
     1861 ttcatgtcag cgattgcgca acattggcaa cttcgctcat gcgatgctgt ccatccatca
     1921 gctcggcaaa ttgcatgcag tcctcgagat gagacgcgta aatatccaag gcacgggcac
     1981 cggcggcgac gagaggctcg agcgcagctg gaactttctc caaaaatcgc tccatgagtc
     2041 gcatattata attcgtatgg ccgacatcaa gggcgacatg ctcatggatg aagctcaagc
     2101 agccgagaac atctgacccg acgagttgga ttatgcgtgc aaaaaacaga gcgccgtagg
     2161 tcacggagat gcgttctatc tcatattcga tggcgatctg acaatagggc gactctccac
     2221 ggatgtactc ttcgtgaagt tcacggtaac ggacgacgtt acgagacaac ggctgtgaca
     2281 ggaacgcctc cggatcaagt ttcgtatcat gtgatgcgtt ccatcgagtc accagggatc
     2341 gcgtgtcagc gatcatcaac tcgtgatggc cagcttcgtg ctcggcgtga cggatcaacc
     2401 cggagcccag ctcttcgtat ccaatcttgc acgtgttctc gccggcgcga cggatccagt
     2461 cctccaccgg ttcggtcatt ccgactcctc gtgaacagaa gtggatcaga aacaagtcag
     2521 ccaccgtcgg tcgcgtttcg ttatggaatg gagtcattcg gtcagcaaaa cgctgcctct
     2581 cggcggctat catgatttca tactgttcga gctcagcgtt catcttgccc ttgccttgaa
     2641 tgcttttgtt tgtcctgtgc acgactcaag atgtcgcgat atctgtcgtc cttgtagaga
     2701 ctcatgagta tgcctcgatc gccatcagag agtttcgata acaagtccgt cttttgattg
     2761 aagaagtaat cgatcgcctc gggatccatc gatggaggag agggcacctc tgtccgagcc
     2821 ggaaatacaa agttcgtcaa cgacgtggcg acgtgcgcag agacttcttt cccatggcga
     2881 gcatgacaca tttcgaggaa ttcccgaata tcaagataga accccaccgc aggcgccccg
     2941 tttacatatt cgcactcgtc aatgacttgt gccgagatcc gccgacaaaa gaggatgcgt
     3001 tcgtggaact cggatctctt cgggtgggtc agcatgagaa tcctctcgat tcccagatgt
     3061 ctactgcaat attcgtacgc gaacttgatc agcggaaaca ggatcatgtt ccaatcacca
     3121 cgatatgtct tcttgaccgc gaaagcagat atttctccca atggaacatt ctctgctctg
     3181 agtcgatcaa tccgatagac acgttcgatc gggaatccca tcgggctatc ttgaatgatc
     3241 gacatggtgc cgatcgcttc accatcccac agcgcaatca ttgtgtgcgt gccggggagc
     3301 gcattgaaga tggtgatgcg cattcccgat gggctgggcc tcatgaaacc catcgagaca
     3361 taagcatcat gaagaatgcg aaaggctgat tctagttcct gtcgtgtttc ggccaccttg
     3421 aaggtgaggt tgctgggtgg cgccaggttc aaatctggat attccggcgc cggttttgag
     3481 ttcaacaagc gcgcgaggag ttcgcgtttc tggcgcggag tgagcgcctc gatgtcagac
     3541 gagtccggca tcccgcgctc cggcgtcgat gagtgtgcgc aaacgtcgat ccacctcgtc
     3601 gtccgacagt tcgtcgacgt tcaccgcgac ctcggtgggt ggctgttccg ctgcgatcgg
     3661 cacgtggcgt gtgctgttcg ccgccgccgg gtgcatggcg gcgagccgct cggccatgaa
     3721 cgccgccgcg ccgctgatgc tgatcccttc catgaacttc atcggcggga tgtccacgcc
     3781 gaattccgtg aggacgcgtt tgcgtatttc gaccgccatc agcgagtcga gaccaagcga
     3841 caccagcgaa cggtcgacct cgatctttga cgcgactgtc ccgagcacac gcgccaggtg
     3901 actacagatg taatcttcga tgagcgcagg acgctcggtg ggagcggcat cgagaatcgc
     3961 gtgcagggca acgtggtctg tcgaggactc gtcggcaccg gccgcagtca tgagcgtgct
     4021 gaggcgcgga gggattcgcg acccgtagag ctgctgtcgc actcgcctcc agtcgatcag
     4081 agcgacgcct gcctggaccg cgccgctctc caggagttga ccgaacgctg caaggatctg
     4141 ctgcaccggc tgacccttca tgccgagccg ctcgacatgc tcctcgacta cgcggttccg
     4201 cgcgacatac ccggtgccgg tgactgcgcc ccagttgatc gtcagtgcgg ggagtccgcg
     4261 gaaacggcga tagtgcgcaa gttcatcgag aaacgcgttg cccgcgacgt agttcgcctg
     4321 tcctggattt ccgatcagtg aggtgaagga cgagaacatg acgaagagat cgagcggatc
     4381 ttccttcgtc aggcgatgga gatgccacgc gcccaatgcc ttgggcagca gcacccgcca
     4441 catctggtcc tgggtcatgt tctggaccag cgtgtcgtcc ggcaccatgg ccgcgtggat
     4501 gatgccgcac agcggagctc cgctggtccg gatcgaggca aggagatcgc cgacctgacg
     4561 ctcgctggtg acatccagcg cagccagcgt cacgcgcgcg cccttgcgtt cgagcgactc
     4621 catatcgtcg gcaatgtccg gcgtcgcctg gccgcgtctc cccgccagca cgaggtgacg
     4681 tgcgccacgg tccaccaggt agtgagcgac tgcgagaccg agaccgccga gcccccccgt
     4741 gatgagatag ctcccatccg cacgggcgcg gaacggttcg gtcggtcgcg cgaccagcgc
     4801 gtctccggtg tcctcgaacg agatcaccac cttgccgacg tgttgggctt gcgcgaggtg
     4861 ccgaaacgcg gcggcggtgc ggtcgagcga aaacaagcgg tacggtaatg gatgcagttc
     4921 tttgttctcg aacgccgcca tcagctcgcc gaacatcgcg cgcatggtct cagcgtgccg
     4981 cgcgccgacg cgatccagat ccaccgagaa gaacgacacg ttgtcgccga acggtctgag
     5041 gctgatgcgg ctgttctcgt agatgtctcg tttgccgagc tccacgaacc gtccgccgtg
     5101 acggagaatg gacaggccct tgggaatcgc ttcgcccgat agtgaattca ggacgatatc
     5161 gactccttcg ccaccggtga tctcgagcac ctcgtcggcg aacgccacgg tgcgtgaatt
     5221 catcacgtgt cgcacgccga gcgccgtcag caggtcgcgc ttcaacggat tgcccaccgt
     5281 ggcgaagacc tcggcacccg cccgctgcgc cagttgaatc gcggccagcc ccacgcctcc
     5341 agtgcccgcg tgaatcaaga ctcgttcgcc gcgctggatg cgcgcaagct tgtgcagcga
     5401 gtagtacgcc gtgacaaacg cgatcggtat cgtcgcgcat tcctcgaagc tcagatgtcc
     5461 cgggttccgg acgacgaatc gcgcatccac ggtcagctgg ctggcgaggc agcccgatcc
     5521 ggacgccagc acggcgtcgc cctcgcgaag cccgtggaca ccgtcgccta cggcggtgat
     5581 tcgccccgag cattcgaggc cgagcatgcc gagcgagtag ccg
//
LOCUS       DASVOR010000293         2360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_294983,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000293 DASVOR010000000
VERSION     DASVOR010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294983"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF516_06160"
     CDS             <1..243
                     /locus_tag="VF516_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107293.1"
                     /translation="FLAARDNEVRKLHSNLSTEFDVLQIESRLTAAQLSAVRARASVL
                     QALTQLRFETGTLIDGKDSGALSSEGLRTIPVAPSR"
     gene            632..796
                     /locus_tag="VF516_06165"
     CDS             632..796
                     /locus_tag="VF516_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107294.1"
                     /translation="MQPEDFKKFEETKGEAKKVIQELGPDELAAVESISAGEGDVVIC
                     NSGCARAPST"
     gene            1089..2159
                     /locus_tag="VF516_06170"
     CDS             1089..2159
                     /locus_tag="VF516_06170"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8107295.1"
                     /translation="MTDSLGPVLRPRMVAALAKLARAGAIEIDDADPEDATLAGAKPR
                     YRLRRLHLELTHRCNFSCRACYLGGRLAAAGDGPGGEVPIERWIALVHEAAALGCTSA
                     VVTGGEPLLRKGVTDLFAALSEHDIRFEVNTNGSCVTPAVVEALRRSTVWSVEVSLYG
                     YDADSHAGYSGVRGGYAATLRGVRLLAAAGVPVQVKYFATAPTAHGLDAVRAELEPLG
                     VPVVCKGHAIHGDIFEGRLPDAAILPELDKSTLVQENELPCGPSLTTLAIAPDGEVRA
                     CPKLGIHFGNVFTEGLTAIWERSTELDAFRSFWADYCHAKGYVRGARTSSLCPAAAAL
                     SQRDGLVQLRTAWNARRDGARS"
     gene            2156..>2360
                     /locus_tag="VF516_06175"
     CDS             2156..>2360
                     /locus_tag="VF516_06175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107296.1"
                     /translation="MTSTVTIYRSPDLWIRFFGEDAALLSRRDGRRRAVDGLTARTLR
                     RLVESLGLSGLERLARDGAGFAEG"
BASE COUNT          362 a          856 c          774 g          368 t
ORIGIN      
        1 ttcctcgccg cgcgcgacaa cgaggtgcgc aagctgcaca gcaacctgtc caccgagttc
       61 gacgtcctcc agatcgagag ccggctcacc gcggcccagc tctcggccgt gcgcgcccgc
      121 gccagcgtcc tccaggcgct cacccagctg cggttcgaga ccggtacgct gatcgacggc
      181 aaggacagcg gcgcgctgtc cagcgagggt ctacggacga tcccggtcgc gccctcgcgc
      241 tagagagcgc ctgcttcgag atccacaggc tcgacgagga acggtcgctc cgtatcacat
      301 gcggaggcgc cgattttcgt tcgatgcgtt acatgcgcat tctgcaccgt gcgcctggcc
      361 cggatctacg cacggttttc gacctgtaga aggcggcgac gcagaagctt cgcgatctgc
      421 ggagtcacgg actcccgatt cggcgccgag ctcctcggaa tcctccaatt ctcttcgtca
      481 tttggtctct gacccgtgca atctccgcca gaactataac ggcatgtcgg ctgcatgggc
      541 ttccgggcat tgcagcgagg gtcgctgcaa ttgcaccgag agcgcgggcc catcgtgggc
      601 gcgctccatt caccttcacg aggagtcttt tatgcaaccc gaagatttca agaaattcga
      661 agagaccaag ggcgaggcca agaaggtgat ccaggagctg gggccggacg agctggccgc
      721 cgtcgagagc atctcggcgg gtgaaggcga cgtcgtgatt tgcaacagcg ggtgcgccag
      781 ggcgccatcg acctagggcg gcagatcgca gatcggacac cgccgctgcg cgtgaggact
      841 gggcccccac gcgcagcgcg cgatccgacc cttcgacata ggcaatccag tcatcgaacg
      901 ggagtcgtat catgatcatc cgcctacgat gcgatcaggc acgcaacgaa gatggatctt
      961 atcagatctc gccgcatccg cttcatcaat cctatgtatt gaatccttac cagctcgcgg
     1021 gcactgccgc gcggctgtgg gaggtggtcg tggcgaccgc tgcgctgccc gcgaacgaac
     1081 agcgcggcgt gaccgactcc ctgggtcccg tgctgcggcc ccgcatggtc gcggcgctgg
     1141 ccaagctggc ccgggcgggc gcgatcgaga tcgacgatgc cgatcccgag gacgccaccc
     1201 tcgccggcgc gaagccgcgc taccgcctgc gcaggctcca cctcgagctg acccaccgct
     1261 gcaacttctc gtgccgcgcg tgctacctcg gcggccgcct cgccgcggcg ggcgacggcc
     1321 ccggcggcga ggtgccgatc gagcgctgga tcgcgctggt ccacgaggcc gccgcgctgg
     1381 gctgcacctc cgccgtcgtc accggagggg agccgctcct gcgcaaggga gtcaccgacc
     1441 tgttcgccgc gctcagcgag cacgatatcc ggttcgaggt caacaccaac ggctcgtgcg
     1501 tcacgccggc cgtggtcgag gcgctgcgcc gctcgacggt gtggagcgtc gaggtctcgc
     1561 tgtacggcta cgacgccgac tcgcacgcgg gctacagcgg cgtgcgcggc ggctacgccg
     1621 ccaccctccg cggcgtgcgg ctgctcgccg ccgcgggtgt ccccgtccag gtcaagtact
     1681 tcgccaccgc acccaccgcg cacggcctcg acgccgtgcg cgccgagctc gagccgctcg
     1741 gcgtgcccgt cgtctgcaag ggccacgcga ttcacggaga catcttcgag ggccgcctcc
     1801 ccgacgccgc catcctgccc gagctggaca agtccacgct ggtccaggag aacgagctgc
     1861 cctgcggccc atcgttgacc acgctcgcga tcgcccccga cggcgaggtc cgcgcctgcc
     1921 ccaagctcgg gatccacttc ggcaacgtgt tcaccgaggg cctgaccgcg atctgggagc
     1981 gatcgaccga gctcgacgcg ttccgctcgt tctgggccga ctactgccac gccaagggct
     2041 acgtgcgcgg cgcccgcacc agctcgctgt gccccgccgc cgcggcgctg tcgcagcgcg
     2101 atggcctcgt ccagctgcgc acggcatgga acgcccggcg cgacggagcg cggtcatgac
     2161 cagcaccgtc acgatctacc ggtcgcccga tctctggatc cggttctttg gcgaagacgc
     2221 cgccctgcta tcgcgccgcg atggccggcg ccgcgcggtc gatggcctga ccgcgcgtac
     2281 gctgcggcgc ctggtcgagt cgctcggcct gagcggcctc gagcgcctcg cccgcgacgg
     2341 tgccggcttc gccgagggcg
//
LOCUS       DASVOR010000294         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353405,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000294 DASVOR010000000
VERSION     DASVOR010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353405"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..749
                     /locus_tag="VF516_06180"
     CDS             378..749
                     /locus_tag="VF516_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107297.1"
                     /translation="MRCGRCNTTCCEAHALAPGERCHRCERDWQEDRVIRCAAKVIFA
                     PPLAIFAGGLLFALFLPISLGGAIGAAVMCALACSMAVATGAGVCRLVDHSARAMFLR
                     EHTAGLPPARLLPAPRGSGHR"
     gene            complement(1119..1883)
                     /locus_tag="VF516_06185"
     CDS             complement(1119..1883)
                     /locus_tag="VF516_06185"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX8107298.1"
                     /translation="MTMPEKFLARHTTASLRALLDDKKPVVALVPVGSTEPHGPHLGL
                     GTDVVISAAACVRATEMFAKKGPLTAVIAPAVSYGVTECARGFAGAVSVPAAVLTAYL
                     AAICDGLLAQGIRHVCLVNNHLEPEHDAAVRAVLPGRDKRVSVACPLTRKWARTLDAE
                     FKSGACHAGLYETSIMMAAAPEMVDDAVRGVLPPVSISLSQQLAAGVTTFAEMGMELA
                     YAGDPASATVEEGERMIQRLAEMVVGEIREALGLPT"
     gene            complement(1908..>3078)
                     /locus_tag="VF516_06190"
     CDS             complement(1908..>3078)
                     /locus_tag="VF516_06190"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8107299.1"
                     /translation="DSLDAAIRAADPAVQLPVVRSDDPAIWLFTSGSTGRPKAAMHSH
                     RDFAFNTEVYARRTVGYTASDVAISVPRLFFGYATGTNLWFPFAVGATVGLFSERPTA
                     ESVADAIARYRPTIVTNVPTMLGKLLDLDDERRAAGGAGLDLSSVRFHLSAGEALPEP
                     LLRRFVERFGGAVYDGIGSAEMFHIYCSNRPDDVVPGSLGRAVDGYTLKILPEDAEGA
                     GAPALPPGEIGVLWVKGDSVAHGYFQDRDKSWKTFHGHWCRTGDLFHIDERGYLWFSG
                     RADDLFKVGGIFVAPREVEDCLLAHPAVSVAAVIPADDQGLVKPKAVIVLRPEARGRD
                     RRALADELKAHVQGRLSKHKYPRWVVFVDDLPKNDRGKVDKKLLIERDRRGDLPE"
BASE COUNT          447 a         1103 c         1068 g          460 t
ORIGIN      
        1 gatcttgcgc cgcaagatca aggacatctg caagaagcac atgcagctcg acgaggcgat
       61 cgatcaggag gtccgcgacc ggatcaagaa cctcgaggag ggcacgtcgg cgtgggaggt
      121 cgagtacgcc aaggttctcg aacagatcaa gcacaagcac ggcgtcaagg agtgacgccg
      181 cgcgctggcg cccgcgcgag cgcgcgcgcc ctggcggggt cgaccggtca actggctctg
      241 gctcgcaccg tctagcagct ggcgcggcct gcgccgcggg tccccacagt atttcgagct
      301 aggcatgcgg ccgcgatggg agcagagtga aagccatggg cgctcccggc tgtacgtcct
      361 gcgtcacgcg cccggtgatg cgctgcgggc gctgcaacac cacctgctgc gaggcacacg
      421 cgctcgcccc cggtgagcgc tgccaccgct gcgagcgcga ctggcaggag gaccgggtca
      481 tccggtgcgc cgccaaggtc atttttgccc caccgctcgc gatcttcgcc ggcggcctgc
      541 tgttcgcgct gttcctgccg atctcgctcg gcggtgcgat cggcgccgcg gtgatgtgcg
      601 cgctggcctg ctcgatggca gtcgcgaccg gcgccggcgt gtgccgcctg gtcgatcaca
      661 gcgcccgcgc catgttcctg cgcgagcaca cggccggcct gccgcccgcc cggctgttac
      721 ctgccccgcg ggggagcggg caccggtaga aacgaccaag cttccccgga gcaggcgccg
      781 agatcgttgg ttccccaagg tgtcgaaagg gggggggctt cgtgcctgcc tcacgcgcat
      841 cgcttgcttg acgtcgacca gggccttgac gttgacgtcc gccgttgagg tcgacgtggc
      901 cgtggacgtt gagcccatcg tcgacctcga cgtggacctc cgtgatcggc acgggtccca
      961 ggcggtcgca tcgcacgacg gtcaaggtgc aaggtcgacg gtggagtcga cgtcgaacgt
     1021 tgccgtcgac gtccaggttc gggtcaacgt catcgtcatc gccaaggaca agaccggaaa
     1081 ttctgtgacg gcttcggtcg tcgtggaccg gcaccgcttc aggtcggcag cccgagcgct
     1141 tcccggatct ctccgaccac catctccgcc agccgctgga tcatccgctc gccctcctcg
     1201 accgtggcag acgccgggtc gccggcgtag gccagctcca tccccatctc ggcgaacgtc
     1261 gtgaccccgg cggcgagctg ctgggacagg ctgatcgaca cgggcggcag cacgccgcgc
     1321 accgcgtcgt cgaccatctc cggcgccgcc gccatcatga tcgaggtctc gtacagcccg
     1381 gcatggcacg cgccgctctt gaactcggcg tcgagcgtgc gcgcccactt cctggtcagc
     1441 gggcacgcga cgctgacccg cttgtcgcgg ccagggagca ccgcccggac cgcggcgtcg
     1501 tgctcgggct cgaggtggtt gttgaccagg cagacgtggc ggatgccctg cgccaggagg
     1561 ccgtcgcaga tcgctgcgag gtacgcggtg agcaccgcgg ccggcaccga caccgccccc
     1621 gcgaagccgc gcgcgcactc ggtgaccccg tagctcaccg ccggcgcgat caccgccgtg
     1681 agcgggccct tcttcgcgaa catttcggtg gcgcggacgc acgccgccgc cgagatcacg
     1741 acgtcggtgc cgagccccag gtgcggcccg tggggctcgg tcgagccgac cggcaccagc
     1801 gcgacgaccg gcttcttgtc gtcgagcagc gcgcgcaggc tcgcggtggt gtgccgggca
     1861 aggaacttct cgggcatcgt cattcgggca gcgttcttcg ggcagcgtta ctcgggcagg
     1921 tcgccgcgac ggtctcgttc gatcaatagc ttcttgtcga ccttcccgcg atcgtttttc
     1981 ggcaggtcgt cgacgaacac cacccatcgc gggtacttgt gcttggacag ccggccctgg
     2041 acgtgggcct tgagctcgtc ggcgagcgcc cggcggtcgc ggccgcgcgc ctcgggacgc
     2101 agcacgatca cggccttggg cttgaccagg ccctggtcat cggccgggat caccgcggcg
     2161 accgacaccg ccgggtgggc gagcaggcag tcctcgacct cgcgcggcgc gacgaagatc
     2221 ccgccgacct tgaacagatc gtcggcccgg cccgagaacc acaggtagcc gcgctcgtcg
     2281 atgtggaaca ggtctccggt ccggcaccag tggccgtgga acgtcttcca gctcttgtcg
     2341 cggtcctgga agtagccgtg cgcgacgctg tcgcccttga cccacaagac gccgatctcg
     2401 cccggcggca gcgccggcgc ccccgcgccc tcggcgtcct cgggcaggat cttcagcgtg
     2461 tagccgtcga ccgcgcggcc gagcgagccg ggcaccacgt cgtccggccg gttcgagcag
     2521 tagatgtgga acatctcggc cgagccgatg ccgtcgtaga ccgcgccgcc gaaccgctcg
     2581 acgaaccggc gcagcagcgg ctcgggcagc gcctcgccgg ccgacaggtg gaaccgcacc
     2641 gacgacagat cgagccccgc cccgcccgcg gcccggcgct cgtcgtcgag gtcgagcagc
     2701 ttcccgagca tggtcggcac gttggtgacg atcgtcggcc ggtagcgcgc gatcgcgtcg
     2761 gccacgctct cggcggtcgg ccgctcgctg aacagcccga ccgtcgcgcc gaccgcgaac
     2821 gggaaccaca ggttggtgcc ggtcgcgtag ccgaagaaca gccgcggcac gctgatcgcg
     2881 acgtcggacg cggtgtagcc cacggtgcgc ctggcgtaga cctcggtgtt gaacgcgaag
     2941 tcccggtggc tgtgcatcgc cgccttgggc cgcccggtcg agcccgacgt gaacagccag
     3001 atcgccggat cgtcgctccg caccaccggc agctgcaccg ccggatccgc cgcgcggatc
     3061 gcggcgtcga gcgagtcc
//
LOCUS       DASVOR010000295         2579 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353408,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000295 DASVOR010000000
VERSION     DASVOR010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2579)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2579)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2579
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353408"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF516_06195"
     CDS             complement(<1..303)
                     /locus_tag="VF516_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8107300.1"
                     /translation="MSTSEYPAQIARRRTFAIIAHPDAGKTTLTEKLLLFGGAIQLAG
                     AVKARGDRRRARSDWMKVERERGISVTTSVMTFDYSDCTFNLLDTPGHEDFSEDTYR"
     gene            491..1024
                     /locus_tag="VF516_06200"
     CDS             491..1024
                     /locus_tag="VF516_06200"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX8107301.1"
                     /translation="MELQPGWHESLHAWKRHGVRFLLVGAHALAAHGRPRFTQDLDVL
                     VDPTPANARRVSAAIAEFGFKETARDWRWFARPYHITMIGRVPMRIDVLTSISGVSFR
                     TAWRNRSVASTTFGEVPVLGLTELRANKLASGRPKDLADVALLDELALAGKPARTKRR
                     KRSAAKGSRRTPRARRQ"
     gene            1328..1684
                     /locus_tag="VF516_06205"
     CDS             1328..1684
                     /locus_tag="VF516_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107302.1"
                     /translation="MRHLVIRAATVLLLFASAAPVAADTIVASTSAVYDTVDAVELHS
                     YRLTITGIVHGTSNATTLTYQILDLNTNFSTEAASRCDRLALLMMAKPGKYQLAMQRP
                     FSSFQGFSCKLTVVAP"
     gene            1699..>2579
                     /locus_tag="VF516_06210"
     CDS             1699..>2579
                     /locus_tag="VF516_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107303.1"
                     /translation="MRTARLVWVKAAVCLVAWVAACGDAPEVPHDRPLRPDAGVPPDA
                     APPPDASPDVPPDAAPVIDIDHDGHLAGEDCDDSDPDVWQNLPYSFRDADGDGHTVAL
                     AGTICSGASLPKGYSRVAGDPDCDDADPNAFTAVTGFLDVDGDGFGDGMAMTFCTAGT
                     LPANYAATGGDCAPADPAAWQDLVYSFRDVDGDGAVVPEAGTVCSGAALPPTYLTSAP
                     AGRPFDCNDGDATVSIPLTIFADADHDGFGTGAGQLTCTNGTPPDGFATTGTDCDDGD
                     PTVWMSLVYTAVDFDGD"
BASE COUNT          400 a          856 c          927 g          396 t
ORIGIN      
        1 gcggtaggtg tcctcgctga agtcctcgtg gccgggggtg tcgagcaggt tgaacgtgca
       61 gtcgctgtag tcgaaggtca tgaccgacgt ggtcaccgag atgccgcgct cgcgctcgac
      121 cttcatccag tcgctgcgcg cgcgccgccg gtcgccgcgc gccttgaccg cgccggcgag
      181 ctggatcgcg ccgccgaaca ggagcagctt ctcggtgagc gtggtcttgc cggcgtcggg
      241 gtgggcgatg atcgcgaacg tgcgtcgccg ggcgatctgc gctgggtact cggacgtcga
      301 catgacggcg caatctacgg tgcgcgcggc ggccgcgcaa cttgctatga tcggcccgtg
      361 agacggatcg cgaagcggac gaccttcgag gacgccgtgg cggacgaggt ccgctactgg
      421 ctcggccgca cgatcgagga gcggatctct gccgtcgagg cgatccggcg tgccagcttc
      481 cggctgtacg gtggagctcc aacccggatg gcacgagtct ctgcacgcgt ggaagcgcca
      541 tggcgtccgg ttcctgctcg tcggcgctca cgcgctggcc gcacacggtc ggccgcggtt
      601 cacgcaggac ctcgacgtcc tcgtcgaccc gacgccggcc aacgcgcgac gcgtgtccgc
      661 ggcgatcgct gagtttggct tcaaggagac cgcgcgagac tggcgctggt tcgccaggcc
      721 gtaccacatc acgatgatcg gtcgcgtgcc gatgcggatc gacgtgctca ccagcatctc
      781 cggggtgagc ttccggaccg cgtggcgcaa ccggagcgtg gcgtcgacga cgtttggcga
      841 ggtgccggtg cttggactca cggagctgcg agccaacaag ctggcgagcg ggcggccgaa
      901 ggacctcgcg gatgtggcct tgctcgacga gctggcgctc gccgggaagc ccgccaggac
      961 gaagcgcagg aaacgctccg cggcgaaagg ttcccgtcga acgccgaggg ctcgccgcca
     1021 atgaacgggg ccatgggtgg gggccacgcc aggacggggt ggcgatcgtg gcgatcgtcg
     1081 tttgccaccg cgcccggacc ggcgggtcgg ctcgatcctg accaggagga ttgctggcca
     1141 cagcggctcc agatatgatt cacaccctgt cgaacgtgga ggcagagatg atgcgaggtg
     1201 gtctgggagg cgatcctgcg tgcggtcggg accggcggtc gacacggcgc cgatcctgga
     1261 gcgcggagca tcgagctcgc tgtgtcactc gtgagacccg acagaacccc tgtctggaga
     1321 agccatgatg cgtcacctgg tcattcgcgc tgccaccgtc ctgctgttgt tcgcgtccgc
     1381 cgcacctgtg gctgcagaca ccattgttgc ctccacgagc gcggtgtatg acaccgtgga
     1441 cgcggtcgag ctgcacagct acagactcac gatcaccggc atcgtccatg gcaccagcaa
     1501 cgccacgacg ttgacctacc agatcttgga cctgaacacg aacttcagta ccgaggctgc
     1561 atcgcgctgc gatcggctcg cgctgctcat gatggccaag cctggcaagt atcagctcgc
     1621 catgcagagg ccgttctcga gcttccaggg gttcagctgc aagctcaccg tcgtcgcgcc
     1681 gtgagctggc cggacgccat gaggacagcg cgtctggtct gggtgaaggc ggcggtctgc
     1741 ctggtcgcgt gggtcgcggc gtgcggtgac gcgcccgagg tgccgcacga taggccgctg
     1801 cggcccgatg caggtgtgcc gccggacgca gcaccgccgc ccgacgcatc tcccgatgtc
     1861 ccgccggacg ccgcgccggt catcgacatc gatcacgacg gccacctggc cggcgaagac
     1921 tgcgacgaca gcgatccgga cgtctggcag aacctgccgt acagcttccg cgacgccgac
     1981 ggcgacgggc acacggtcgc gctggccggg acgatctgca gcggagcgag cctgcccaag
     2041 ggctactcgc gggtcgccgg tgacccggac tgcgacgatg ctgatccgaa cgcgttcacg
     2101 gcggtgaccg gcttcctcga cgtcgacggc gacgggttcg gcgacggcat ggcgatgacg
     2161 ttctgcacgg ccggcaccct gccggcgaac tacgcggcga ccggcgggga ctgcgcgccg
     2221 gcggatccgg cggcgtggca agacctggtg tacagcttcc gcgacgtcga tggtgacggc
     2281 gcggtggttc cagaagccgg cacggtgtgc agcggcgcgg cgctgccgcc cacgtacctg
     2341 acgtcggcgc cggcgggccg gccgttcgac tgcaacgacg gcgacgccac cgtatcgatc
     2401 ccgctgacga tcttcgccga cgcggatcac gacggcttcg ggaccggcgc ggggcagctg
     2461 acgtgcacga acggcacgcc gccggacggg ttcgccacca ccggcaccga ctgcgacgac
     2521 ggcgacccca cggtgtggat gtcgctggtg tacacggcgg tcgacttcga cggcgataa
//
LOCUS       DASVOR010000296         4804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_440992,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000296 DASVOR010000000
VERSION     DASVOR010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440992"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..2091
                     /gene="ligA"
                     /locus_tag="VF516_06215"
     CDS             43..2091
                     /gene="ligA"
                     /locus_tag="VF516_06215"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEX8107304.1"
                     /translation="MSSDPHAEYLALVDELTEHDRRYYVEANPTISDVEYDRLSQRLR
                     AIEAEHPDWVVAWSPSQRIGHAPVSDFPKVHRPVAMLSLDNTYDEDELRAFFDRVVKG
                     LDGDVPVFSVEPKIDGFGIELTYKAGTLVLGATRGDGRIGEDVTANVRMVRGVALRLR
                     EPVDIVVRGEIYMTKQEFAAINAARIEAGEEPFKNPRNTAAGSIKQLDPREVAKRPMR
                     TILYEVVDGERYAPGHLASLARLGRLGLPVSAHNTQAASWDELIAGVHSWRDRRDELA
                     YEVDGLVIKVDDFGLRAALGTTAKFPRWAIAYKFPARQVTTVLRDIHSYVGRTGAVTP
                     VAILDPVELSGTTVGRASVHNWDIVAKLGLGKGDRVLLEKAGEIIPQILAVTEKGEGP
                     AFPAPTECPSCHTPLIREEGKVALVCPNRLACPAQQLAAIEFFASRGQMNIDGLGEKV
                     VAQLVDTGLVRDVSDLFVLTAEQLENLERFAEQSARNLVAAIARARADATFARLLAAL
                     GIPNLGGVLARPIAEKYGKLSALVVRAEQLDSDALVAELCEIDGIGETIAQSIDRFLR
                     DPHTAVVLRKLAERGVDPEQPARVVADGPLSGKTLVVTGTLAAPRAEVQRRIEAAGGK
                     VAGSVSKKTSYLVAGADTGKSKLEAAQKHGVQVISEQELEQLLAGAADTDAPAPPSES
                     "
     gene            complement(2092..2889)
                     /locus_tag="VF516_06220"
     CDS             complement(2092..2889)
                     /locus_tag="VF516_06220"
                     /inference="COORDINATES: protein motif:HMM:NF028004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P460 family protein"
                     /protein_id="HEX8107305.1"
                     /translation="MLTRQTLHIVGVLAGSLSLWLGLPAAAHAGGDATAAAAKSQACE
                     ACHLATTGDTPQLAGQRETYIVKQLKAFKAGDRKHPVMNAITSVLSEADMDNLAAAWN
                     KRSPTDTTVPPAVAAAQKTKMTFPKDFPKGFVLYRTGAKPDGATVAKQYINNAGFAAV
                     KAGKDKLPDGTVILVENFSKKPGAAKDSLETDALKGYEGMEARAGWDKGFPELLMNHD
                     WNYAVFAPDKSVRNEVNQAICLSCHIPAAGKDYVFTLDHIQDKAKGK"
     gene            complement(3260..3997)
                     /locus_tag="VF516_06225"
     CDS             complement(3260..3997)
                     /locus_tag="VF516_06225"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8107306.1"
                     /translation="MMLRKFAILAALVPALASAAPADTPPTNTPTNNAPTNNTPNNHP
                     ADPTPDKSPPKGTTSSSDTSSNKTAGNAEKGAKLSDADIKVIAHLHHVNQMEITLAKE
                     AQRQGTAKVKDYANMMITDHQQVDKDLTAFAKAHHLTTIPADKPTTDAERQDDKDMTQ
                     AMANLKKLKGADFDKAYLNMMVSGHDKELTKIDVAISSTSDPDLKSMLQNVKPVLQRH
                     ADQARDLLKSPQASADQRAPHPASSSR"
     gene            complement(4142..4309)
                     /locus_tag="VF516_06230"
     CDS             complement(4142..4309)
                     /locus_tag="VF516_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107307.1"
                     /translation="MAAQLERDLGVSTKLVPGNTGELSVWVDDAQVIAKQGAKFPDPG
                     DVVAAVRAHRT"
     gene            4487..>4804
                     /locus_tag="VF516_06235"
     CDS             4487..>4804
                     /locus_tag="VF516_06235"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8107308.1"
                     /translation="MWLAHALTLLRIPLAAALWWTWGDPVWSVAIVAVAALTDTADGR
                     VARWAQRRGARGPDIGGWLDPAVDKLFVAIVLAAIWVHTWIPHKTAVIALTGARELVL
                     VPMV"
BASE COUNT          651 a         1622 c         1748 g          783 t
ORIGIN      
        1 cgagccggct ggcgttgtga tcgcgggtat gcttcggcgg ccatgtcctc cgatccgcac
       61 gccgagtatc tggcgctggt cgacgagctg accgagcacg accggcgcta ctacgtcgag
      121 gccaacccca cgatcagcga cgtcgagtac gaccggctga gccagcggct gcgcgcgatc
      181 gaggccgagc accccgactg ggtggtcgcg tggtcgccga gccagcggat cggccacgcg
      241 ccggtctcgg acttccccaa ggtccaccgg ccggtcgcga tgctgtcgct cgacaacacg
      301 tacgacgagg acgagctccg ggcgttcttc gaccgcgtgg tcaaggggct cgacggcgac
      361 gtgccggtgt tctcggtcga gcccaagatc gacgggttcg ggatcgagct gacctacaag
      421 gccgggacgc tggtgctcgg cgcgacccgc ggcgacggcc ggatcggcga ggacgtgacc
      481 gccaacgtcc ggatggtgcg cggcgtcgcg ctccggctgc gcgagccggt cgacatcgtc
      541 gtgcgcggcg agatctacat gaccaagcag gagttcgcgg cgatcaacgc ggcgcggatc
      601 gaggccggcg aggagccgtt caagaacccg cgcaacaccg cggccggctc gatcaagcag
      661 ctcgatccgc gcgaggtcgc caagcggccg atgcgcacca tcctctacga ggtggtcgac
      721 ggcgagcgct acgcgcccgg ccacctggcg tcgctcgcgc ggctcggccg gctcggcctg
      781 ccggtgtcgg cgcacaacac ccaggccgcg agctgggacg agctgatcgc cggcgtgcac
      841 agctggcgcg atcgccgcga cgagctggcg tacgaggtcg acggcctggt gatcaaggtc
      901 gacgatttcg ggctgcgcgc cgcgctcggc accaccgcga agttcccgcg ctgggcgatc
      961 gcgtacaagt tcccggcccg ccaggtcacc accgtgctgc gcgacatcca cagctacgtc
     1021 ggccggaccg gcgcggtcac cccggtcgcc atcctcgatc cggtcgagct gtccggcacc
     1081 accgtcgggc gcgcctcggt ccacaactgg gacatcgtcg cgaagctcgg cctgggcaag
     1141 ggcgatcgcg tgctgctcga gaaggccggc gagatcatcc cgcagatcct cgcggtcacc
     1201 gagaagggcg agggccccgc gttccccgcg cccaccgagt gcccgtcgtg ccacacgccg
     1261 ctgatccgcg aggagggcaa ggtcgcgctg gtctgcccca accggctcgc ctgcccggcg
     1321 cagcagctcg ccgcgatcga gttcttcgcg tcgcgcggcc agatgaacat cgacggcctg
     1381 ggcgagaagg tcgtcgccca gctggtcgac accggcctgg tccgcgacgt gtccgacctg
     1441 ttcgtgctga ccgccgagca gctcgagaac ctcgagcggt tcgccgagca gtcggcgcgc
     1501 aacctggtcg cggcgatcgc cagggcccgg gccgacgcca cgttcgcccg gctgctcgcg
     1561 gcgctcggca ttcccaacct cggcggcgtg ctcgcccggc cgatcgccga gaagtacggc
     1621 aagctcagcg cgctggtcgt gcgggccgag cagctcgact ccgacgcgct ggtcgccgag
     1681 ctgtgcgaga tcgacggcat cggcgagacc atcgcgcaga gcatcgaccg cttcctgcgc
     1741 gatccgcaca ccgccgtggt gctgcgcaag ctcgccgagc gcggcgtcga tcccgagcag
     1801 ccggcgcggg tcgtcgccga tggcccgctg tcgggcaaga cgctggtggt caccggcacg
     1861 ctggccgcgc cgcgcgccga ggtccagagg cggatcgagg ccgccggcgg caaggtcgcc
     1921 ggctcggtca gcaagaagac cagctacctg gtggccgggg ccgacaccgg caagtccaag
     1981 ctcgaggccg cccagaagca cggcgtccag gtgatcagcg agcaggagct cgagcagctg
     2041 ctcgccggcg ccgccgacac cgatgctccg gcgccgccga gcgagtcctg actacttgcc
     2101 cttggccttg tcctggatgt ggtcgagcgt gaacacgtag tccttcccgg ccgccgggat
     2161 gtggcaggac aggcagatcg cctggttgac ctcgttgcgc accgacttgt cgggggcgaa
     2221 cacggcgtag ttccagtcgt ggttcatcag cagctcgggg aagcccttgt cccagccggc
     2281 gcgcgcttcc atgccctcgt agcccttgag cgcatcggtc tcgaggctat ccttggccgc
     2341 gcccggcttc ttgctgaagt tctcgacgag gatgaccgtg ccgtcgggca gcttgtcctt
     2401 gcccgccttg accgccgcga acccggcgtt gttgatgtac tgcttggcga cggtggcccc
     2461 gtcgggcttg gccccggtgc ggtagagcac gaagcccttg gggaagtcct tggggaacgt
     2521 catcttggtc ttctgcgcgg cggcgaccgc gggcgggacg gtggtgtcgg tcggcgagcg
     2581 cttgttccag gccgccgcga ggttgtccat gtccgcctcg ctgagcacgc tggtgatcgc
     2641 gttcatcacc gggtgcttgc ggtcgccggc cttgaacgcc ttgagctgct tgacgatgta
     2701 agtctcgcgc tggccggcga gctgcggcgt gtcgccggtg gtggcgaggt ggcaggcctc
     2761 gcaggcctgg ctcttggccg cggccgcggt ggcatcgcct ccggcgtggg ccgccgcggg
     2821 aagaccgagc cagagagaga ggctaccggc gaggacgccg acgatgtgga gggtctgacg
     2881 cgtgagcatg acggactcct gacaagggaa gttcgatgaa cgcgcgcagt gtgccaccac
     2941 ttgcggccgg aatgcgagaa tcatggaacg atcgtcacgg tgtggcgggc aaacgcgtgc
     3001 cgccgcgaaa cgcggcgcag cgagagcccg ccagcgagca tgacgccgcc ggccatggga
     3061 acgatggcga tcgcgggggc gcgggtcggc gctcgacgac agcccggtcg acgtctcggc
     3121 gtcgccggtt tctggcgcgc gaccgacttc ggcgacacca cgctgacgcc cggcgaaggg
     3181 cgccagctcg ccgccgatcc cggcttcctg cgcgcgcagt cccagcgccc gcagccgtgc
     3241 gtcgccgatc cggtcggcgc tatcgactcg acgacgccgg gtgcggcgcc cgctggtcgg
     3301 ccgaggcctg cgggctcttc agcagatcgc gcgcctggtc ggcgtggcgc tgcagcaccg
     3361 gcttgacgtt ctgcagcatg ctcttgaggt ccggatcgct ggtcgagctg atcgcaacat
     3421 cgatcttggt cagctccttg tcgtgacccg acaccatcat gttgaggtag gccttgtcga
     3481 agtcggcgcc cttgagcttc ttcaggttcg ccatcgcctg ggtcatgtcc ttgtcgtcct
     3541 ggcgctcggc gtcggtcgtc ggcttgtcgg ccgggatcgt cgtcaggtga tgcgccttgg
     3601 cgaacgccgt caggtccttg tccacctgct gatggtccgt gatcatcatg ttggcgtagt
     3661 ccttgacctt cgccgtgccc tggcgctgcg cctccttcgc gagcgtgatc tccatctggt
     3721 tgacgtgatg gaggtgggcg atgaccttga tgtcggcgtc gctgagcttc gcgcccttct
     3781 cggcgttgcc ggcggtcttg ttcgacgagg tgtcgctcga gctcgtcgtg cccttgggcg
     3841 gcgacttgtc gggagtcgga tcggccggat ggttgttcgg cgtgttgttg gtcggcgcgt
     3901 tgttggtcgg cgtgttggtc ggcggagtat ccgccggcgc ggcgctcgcg agcgcgggca
     3961 cgagcgcggc gaggatggcg aatttgcgaa gcatcatgac agtccccttg gttaaatccg
     4021 cagtgggccg ttgcaagtgg ggtaccgtcg ggcgcgaccg cgattccagc agacttgtcg
     4081 cctgcgccgc ccgctcatcc gttggcgtgg acggggcgcg ccggcacgtc gcgcgggggt
     4141 gtcacgtccg gtgcgcgcgg acggccgcca ccacgtcgcc gggatcgggg aatttcgcgc
     4201 cctgcttggc aatgacctgt gcgtcgtcga cccagacgct gagctcgccg gtgttgccgg
     4261 gaacgagctt cgtcgagact cccagatcgc gctcgagctg cgctgccacc cgggcagcgt
     4321 acggccgata gcctcaggac gtgcaatagc ggatcgtgat caccatgccg ggagcgtagc
     4381 acgctgcgat ccagcgccgg aaccgccgcg ccgggggctc gccgagaccg cggcgctgcg
     4441 ggaagccgcg atgccggacc gcgccggtcg atgctagatg cagggcgtgt ggctcgcaca
     4501 cgcgttgacg ctgttgcgga tcccgctcgc ggccgcgctg tggtggacat ggggcgatcc
     4561 ggtgtggtcg gtggcgatcg tcgcggtcgc ggcgctgacc gacaccgccg acggccgggt
     4621 cgcgcggtgg gcgcagcggc gcggcgcgcg cggtccggat atcggcggct ggctcgatcc
     4681 ggcggtcgac aagctgttcg tcgcgatcgt gctggcggcg atctgggtcc acacctggat
     4741 cccgcacaag accgcggtga tcgcactgac cggggcgcgg gagctcgtgc tggtcccgat
     4801 ggtc
//
LOCUS       DASVOR010000297         5193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_178186,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000297 DASVOR010000000
VERSION     DASVOR010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178186"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3276)
                     /locus_tag="VF516_06240"
     CDS             complement(<1..3276)
                     /locus_tag="VF516_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8107309.1"
                     /translation="MDTPAVVGGRFAIRRVAGTGGMGRVFEALDLLTGANVALKLMQD
                     PSAPRELERFTRESETLAGLRHPGIVRYVAHGQTETGEGYLAMEWLDGEDLRQRMEER
                     GLSVSETMLLARQVADALAAAHKAGVIHRDLKPSNLFLVGGDVGNIKIIDFGVARRSA
                     RAQALTQTGAQVGTPQYMAPEQARGERDIKPAADVFSLGCILYECLAGQPPFFADHIA
                     ALLAKILFEEARPLDTVCPHVPRALCDLIGRMLAKEPGARPSDAAALRDELSALSPIS
                     PELDVAPSRASRAAAPAITAREQYLVSVVVARPLDETSALSPTLDPSSEREAHSQREM
                     LEPLLRQFGVRVEGLADGTLVATSALAQVGNAADLALQIARCAILIHERWPEAVVALA
                     TGRAQVDVFGHIPVGEAIDRAVRLTRRNTPTVHLKAGEVERLVDGVLIDEASAHLLRP
                     RFLTTTTQNEIVLQGERVGVDDTRLLLGKPTPCVGREPELALLELHLQRCIDESKAHA
                     LVVIAPPGVGKSRLRHEFLRRIEGRDCQVLIGRGDPVSAGSPYGLLAQALRLLCEIQL
                     GDAADAAQGKLRARVAVHVPAADVDRVTEFLGELCGLPFPDEGRTLLRAARADPKVMS
                     DQVGRAFVDLIGAECAVRPLLLVLEDLHWSDGLTVKLVEAALRELELPLFVLALARPD
                     ALDMFPNLWADHHDVLSLREMSARACERLVRQVLGKTAPDELVTRLVKRSAGNALFLE
                     ELIRAAAEGKGERLPETVLAILQTRLTRLDAPTRRLLRAGAVFGTTFWRDGVAELCGL
                     DRASPELDANLAVAVDQEMIERAKESRFASEIQYAFRHALMRDAAYGLLTDQDRREGH
                     RHAAQYLERIGEPDPGVLADHYREGDDPAAAAPLYCRAADQAVAGHDLATALRRVELG
                     LKCGPEGKLLGAMRGLQCYAHFWNDQWETAYPFGSEALELLPAGSYRWCKTMGTMLTI
                     TSLSGKLYHFGRLVEVFAAVEVDPEARTAYVEAGALLAVMFAYLGRRTQSRLFVERIE
                     AIGGLQGDDDATTAGWVHFARVYYSRLLGSDPWHTLAISRQGSDAFEKAGSTRNYVFL
                     NNFT"
     gene            complement(3539..>5193)
                     /locus_tag="VF516_06245"
     CDS             complement(3539..>5193)
                     /locus_tag="VF516_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HEX8107310.1"
                     /translation="PRAVAPTPGAWHTPAIAGASSDALKALVWACSTLDDESLAGAIG
                     DLAVRCFTKIRGVGALSMKVGNACIYALCQLPGLRAVAQLSRLSARVRYKQALALIDR
                     AKLACARRAGMDPIDLEELAVPTFGIPGRIELGAHSAELAIDGDAAELVFHAGDRRLR
                     TVPAEVKQGHAEELEELRASHKELAALLPALRARLERWMLEPRRWALPDLRARYLDHP
                     VVGALARRLIFTTGSTSVLFGEDGALDATGARVALPDDAVLALWHPLGRPVAEVRAWR
                     ERLEALAITQPFKQAHREIYLLTDAERQTEIYSNRFAAHLVRQHQFAALCRERGWTYT
                     LQGHWDSHSTPRRHVAAYDLDVEFWVEAGDEAPVSDAAVYLYVATDQVRFLRGGRPLR
                     LDDVPPRCFSELMRDVDLFVGVCSIGADPTWRDHGPRGTFGYWEQYAFGDLGASGETR
                     KAALERLLPRLKIRDRVRIDGRFVVVRGQLRTYKIHLGSGNILMEPNDHYLCIVPDRA
                     RPIDVKLPFEGDHLLSVILSKALLLAADDQIQDPSIARQIAS"
BASE COUNT          718 a         1831 c         1952 g          692 t
ORIGIN      
        1 ggtgaagttg ttgaggaaga cgtagttgcg ggtcgagccg gccttctcga acgcatcgct
       61 gccctgccgc gagatcgcca gggtgtgcca cgggtcgctg ccgagcagcc gcgagtagta
      121 gacgcgcgcg aagtggaccc agccggcggt ggtggcgtcg tcgtcgccct gcaggccgcc
      181 gatcgcctcg atgcgctcga cgaacagccg ggactgggtg cgccggccga ggtaggcgaa
      241 catcaccgcg agcagcgcgc cggcctcgac gtacgcggtg cgggcctcgg ggtcgacctc
      301 gacggcggcg aacacctcga ccagccgccc gaagtggtag agcttgccgg acaggctggt
      361 gatcgtgagc atcgtgccca tcgtcttgca ccagcggtac gagccggcgg gcagcagctc
      421 cagcgcctcg ctgccgaacg gatacgcggt ctcccactga tcgttccaga agtgcgcgta
      481 gcactgcagg ccgcgcatcg cgccgagcag cttgccctcg gggccgcact tgaggccgag
      541 ctcgacccgc cggagcgcgg tcgcgaggtc gtggccggcg accgcctggt cggcggcgcg
      601 gcagtacagc ggcgcggcgg cggccggatc gtcgccctcg cggtagtgat cggcgagcac
      661 gccgggatcg ggctcgccga tccgctcgag gtactgcgcg gcgtggcggt ggccctcgcg
      721 gcggtcctgg tcggtgagca ggccgtaggc ggcgtcgcgc atcagcgcat ggcggaacgc
      781 gtactggatc tcgctggcga accggctctc cttggcgcgc tcgatcatct cctggtccac
      841 cgcgacggcg aggtttgcgt cgagctcggg gctggcgcgg tcgagcccgc acagctcggc
      901 gacgccatcg cgccagaacg tggtgccgaa caccgcgccg gcgcgcagca ggcgccgggt
      961 cggcgcgtcg agccgggtca gccgggtctg caggatcgcg agcacggtct cgggcagccg
     1021 ctcgcccttg ccctcggcgg cggcgcggat cagctcctcg aggaacagcg cgttgccggc
     1081 cgagcgcttg accagccggg tgacgagctc gtcgggcgcg gtcttgccga gcacctggcg
     1141 gaccagccgc tcgcacgccc gggcgctcat ctcgcgcagc gacagcacgt cgtggtggtc
     1201 ggcccacagg ttggggaaca tgtcgagcgc gtcggggcgc gccagcgcga gcacgaacag
     1261 cggcagctcg agctcgcgca gcgcggcctc gaccagcttg acggtcaggc cgtcgctcca
     1321 gtgcaggtcc tcgagcacga gcagcagcgg acggaccgcg cactcggcgc cgatcaggtc
     1381 gacgaacgcg cggccgacct ggtcgctcat caccttggga tcggcgcgcg cggcgcgcag
     1441 cagggtgcgg ccctcgtcgg gaaacggcag gccgcacagc tcgccgagga actcggtgac
     1501 ccggtcgacg tcggcggcgg ggacgtgcac ggcgacccgc gcgcgcagct tgccctgcgc
     1561 cgcgtcggcg gcgtcgccga gctggatctc gcacagaagg cgcagcgcct gggcgagcag
     1621 gccgtagggc gagcccgcgc tgaccggatc gccgcgcccg atcaggacct ggcagtcgcg
     1681 cccttcgatc cggcgcagga actcgtggcg cagccgcgac ttgccgacgc cgggcggcgc
     1741 gatcaccacg agggcgtggg ccttggactc gtcgatgcag cgctggaggt ggagctcgag
     1801 cagcgcgagc tcgggctcgc ggccgacgca cggggtcggc ttgccgagca ggaggcgggt
     1861 gtcgtcgacg ccgacccgct cgccctgcag cacgatctcg ttctgggtcg tggtggtgag
     1921 gaaccgcggg cggagcaggt gcgcgctggc ctcgtcgatc agcacgccgt cgaccaggcg
     1981 ctcgacctcg ccggccttga ggtggacggt cggcgtgttg cgccgggtca gccggaccgc
     2041 gcgatcgatc gcctcgccga ccgggatgtg gccgaacacg tcgacctggg cgcggccggt
     2101 cgcgagcgcg accaccgcct cgggccagcg ctcgtggatc aggatcgcgc agcgcgcgat
     2161 ctgcagcgcg aggtcggcgg cgttgccgac ctgggcgagc gccgaggtcg cgaccagcgt
     2221 gccgtcggcg aggccctcga ctcggacgcc gaactggcgg agcagcggct cgagcatctc
     2281 gcgctggctg tgcgcctcgc gctccgacga cggatcgagc gtgggcgaca gcgcgctggt
     2341 ctcgtccagc gggcgcgcga ccacgacgga caccaggtat tgctcgcgcg cggtgatcgc
     2401 cggcgcggcg gcgcgcgacg cgcgggacgg cgcgacgtcg agctcggggc tgatcgggga
     2461 cagcgcgctc agctcgtcgc gcagcgccgc ggcgtcgctc ggccgcgcgc cgggttcctt
     2521 ggcgagcatc cggccgatca ggtcgcacag cgcgcgcggc acgtgcgggc acacggtgtc
     2581 gagcgggcgc gcctcctcga acaggatctt ggcgagcagc gccgcgatgt ggtcggcgaa
     2641 gaacggcggc tggccggcca ggcactcgta gaggatgcag ccgagcgaga acacgtcggc
     2701 ggcgggcttg atgtcgcgct cgccgcgcgc ctgctcgggc gccatgtact gcggtgtgcc
     2761 gacctgcgcg ccggtctggg tcagcgcctg ggcgcgcgcc gagcggcggg cgacgccgaa
     2821 gtcgatgatc ttgatgttgc cgacgtcgcc gccgaccagg aacaggttcg acggcttgag
     2881 gtcgcggtgg atgacgccgg ccttgtgcgc cgcggccagc gcgtcggcga cctggcgggc
     2941 gagcagcatg gtctcggaca ccgacaggcc gcgctcctcc atccgctgtc gcagatcctc
     3001 gccgtccagc cactccatcg ccagataacc ctcaccggtc tcggtctggc cgtgggcgac
     3061 gtagcggacg atcccggggt ggcggagccc ggcgagcgtc tcggactcgc gggtgaagcg
     3121 ctcgagctcg cgcggagcgc tcggatcctg catcagcttg agggcgacgt tggcgccggt
     3181 cagcaggtcg agcgcctcga agacacggcc catcccgccg gttccggcga cccgccggat
     3241 cgcgaagcga ccaccgacca ccgcaggcgt atccattccg gaacgaatct accaggttgg
     3301 tggatcgctg ccgagtgatt cccctgtggt gtgacgtggg cgcgcacggt tacacccgga
     3361 tcgaagctgg gtccgaccgg tgccggagga agaacgtcgt tccagcgccg gccagcgccg
     3421 gccagcgccg gccagcgccg gccagcgccg gatcgagtca gcggtcgggc tgtcgggtga
     3481 cccgcgactc gtcctacgca tggcgagatc cacgggcacg cctcacgcta gcacgcggtc
     3541 acgaggcgat ctggcgcgcg atgctggggt cctggatctg gtcgtccgct gcgagcagga
     3601 gcgccttgga caggatcacc gacagcaggt gatcgccctc gaacggcagc ttcacgtcga
     3661 tgggccgcgc ccgatccggg acgatgcaca ggtagtggtc gttgggctcc atcaagatgt
     3721 tgccggagcc gaggtggatc ttgtaggtgc gtagctggcc gcgcacgacg acgaaacgcc
     3781 cgtcgatccg cacgcggtcc cggatcttga gcctgggcag cagccgctcg agcgcggcct
     3841 tgcgggtctc gccggatgcg ccgagatcgc cgaacgcgta ctgctcccag tacccgaacg
     3901 tgccccgcgg tccgtggtcg cgccaggtcg ggtcggcgcc gatgctgcac acgccgacga
     3961 acagatcgac gtcgcgcatc agctcggaga agcagcgcgg cgggacgtcg tcgagccgca
     4021 gcggcctgcc gccgcgcagg aaccggacct ggtcggtcgc gacgtagagg tagactgccg
     4081 cgtcactgac cggagcctcg tcgccggctt ccacccagaa ctcgacgtcg agatcgtacg
     4141 cggcgacgtg gcgccgcggc gtgctgtgcg agtcccagtg cccctgcagg gtgtacgtcc
     4201 agccccgctc gcggcaaagc gcggcgaact ggtgctggcg gaccaggtgc gccgcgaacc
     4261 ggttcgagta gatctcggtc tgccgctcgg cgtcggtgag caggtagatc tcgcgatgcg
     4321 cctgcttgaa cggctgcgtg atcgccaggg cctcgagccg ctcgcgccac gcgcggacct
     4381 cggcgaccgg gcggccgagc ggatgccaca gcgcgagcac cgcgtcgtcg ggcagcgcga
     4441 cccgcgcgcc ggtggcatcg agcgcgccgt cctcgccgaa cagcacgctg gtcgatccgg
     4501 tcgtgaagat cagccgccgc gcgagcgcgc cgaccaccgg atggtcgagg tagcgcgccc
     4561 gtagatccgg cagcgcccag cgccgcggct cgagcatcca gcgctccagc cgcgcgcgca
     4621 gcgccggcag cagcgcggcg agctccttgt gcgacgcgcg cagctcctcg agctcctccg
     4681 cgtggccctg cttgacctcg gcggggaccg tccggagccg gcgatcgccg gcgtggaaca
     4741 ccagctcggc ggcatcgccg tcgatcgcga gctccgcgct gtgcgcgccg agctcgatgc
     4801 gccccgggat cccgaacgtc ggcaccgcca gctcctcgag atcgatcggg tccatgccgg
     4861 cgcgccgcgc gcacgcgagc ttggccctgt cgatcagcgc gagcgcctgc ttgtagcgca
     4921 cgcgcgccga cagccgcgac agctgcgcga ccgcgcgcag gccgggcagc tggcacagcg
     4981 cgtagatgca cgcgttgccg accttcatcg acagcgcgcc gacgccgcgg atcttcgtga
     5041 agcagcgcac cgcgagatcg ccgatcgccc cggccagcga ttcgtcgtcg agcgtgctgc
     5101 acgcccacac cagcgccttg agcgcgtcgc tgctggcgcc ggcgatcgcc ggcgtgtgcc
     5161 acgcacccgg ggtcggcgcg acggcgcgcg gcg
//
LOCUS       DASVOR010000298         4600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382577,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000298 DASVOR010000000
VERSION     DASVOR010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382577"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..679)
                     /locus_tag="VF516_06250"
     CDS             complement(224..679)
                     /locus_tag="VF516_06250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107311.1"
                     /translation="MKRREPLNLAHVLEDRSPSAIPVPIIEKRIHTRFDKAFLVVIGS
                     ELYGDTTAVARNISAGGILVEMTYAPPLGTVVTVHFQHVRYDDPLERSETGGRAPNGI
                     DELVVRAEVKHHHYLNFTGGNDAASARAIGLRFLEFIDPDERIPPDRWH"
     gene            805..1629
                     /locus_tag="VF516_06255"
     CDS             805..1629
                     /locus_tag="VF516_06255"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8107312.1"
                     /translation="MLCVVASTAVASIAVASPRASNPAFLGIQMIDASGGRGPCVIEG
                     ATRDGPAEAAGLRSGDRVLTVDGKRIANCSALLDEITAHAPGETVEVKVERLGRALVV
                     KAQLTTRDALLHHMIGKPMIATDLVGVEDGETYDLSALHGQMAIVGLYNPACVDCASL
                     FGKFLDWASDRSRREQPPLVLAVVPSAEPTHDLRALQKSLDVPLAVGEFSPGGFGGSP
                     FARDMLISDRDRLGVIVIDGRGTVQYLGPVAPNSDDTEAVLDEVFAAAELAWRRSR"
     gene            1637..2176
                     /locus_tag="VF516_06260"
     CDS             1637..2176
                     /locus_tag="VF516_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEX8107313.1"
                     /translation="MTAVIRSFGGKRPQIGRGVFLAETCALIGDVVIGDESSIWYGVV
                     LRGDMYPIRVGARTNLQDGTIVHITGGQFATTIGDDCTVGHGAIVHGCTIEDHCLIGM
                     GATILDGARVGRGSLVGAGALVTPGTDIPPNSLVLGAPAKVVRSVHPKETEQIEYGAV
                     HYVELARRYLAGDADSSVP"
     gene            2250..3191
                     /locus_tag="VF516_06265"
     CDS             2250..3191
                     /locus_tag="VF516_06265"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8107314.1"
                     /translation="MTSSRVFPIARAAVRVVSIAVALVVAGRATPARADNVDKLVHQL
                     GDDDSDKVRLSAALNLTKLGDARVSVISALTKALGNDTDEGVRGASAVGLGKLVTDKT
                     APKVRNAAVDALTQASQSDPSAFVRGEAGKALKAIGVTAQVQTPQAGTAIYVNIGPMA
                     AKTGDPAVDARLKALMVKVANQTMTQVASTMAITWPGGAPPTQAALAAKSTIGFYVDG
                     TVNELKMKPAGSSTTVSCKINMLLADYPNKSIFGLLTGSASVEASGSASSLALAGEDC
                     VSKLVENLIATKIVPTINSKVGTPPPPSTSSKVGKTP"
     gene            3188..4030
                     /locus_tag="VF516_06270"
     CDS             3188..4030
                     /locus_tag="VF516_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107315.1"
                     /translation="MTKPGDRGYKRSWKNLLINKRYQLRFTLFMVGLSALLMVGLGAW
                     VMWVADETTAVSITSVRGDACPKLPAVNATGATSEDDPAVHAPSMKLDEADGAAAPAP
                     GSAAGSAADNEEPRHVKVQIDESSMTLTPTLPPDFADRVTAHWACELRQAATFDRLER
                     GRLRILWAMIATGIALVIGLALYGIKMTHRVAGPLFKVGLYLSKMRDGRFDKVYNLRK
                     GDQLVDFYDQFKAAHGGIVAMQQDDIARIAQVIAAADAAGLGQHPVVAELRALSARKE
                     KSIE"
     gene            4023..>4600
                     /locus_tag="VF516_06275"
     CDS             4023..>4600
                     /locus_tag="VF516_06275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107316.1"
                     /translation="MSDPTAATGLPSPVPRSKHQRKLSNYLLDKNLQLRYILLVTLLS
                     AAISGALGYMIYDQSRLASESIERDLQVLTVGDTSRQEFTSILAGKDQMLIYEMVALG
                     LGLVVILSAYLVIMTHKVAGPLFKTSIYFDRMAEGRLSAVTPLRRGDMLQDFFGNFKA
                     MHESLRVRAEGDVEIMERAAAAMRKAQDEADYR"
BASE COUNT          749 a         1534 c         1637 g          680 t
ORIGIN      
        1 gccagcgctc gtcgccgccg gctcggcggc cgcgcgcttg tgacgggccg cgcggggcgc
       61 cgcggcgacg ctccccgagg agaccacgcc ggcgagcagg accaggacca gggcgccgcg
      121 catcgcggag gacttagcca atcgagccac ggcgcgcaag atagcgttgc aacgtcgtgc
      181 ggcaggcggt gattccccgg gctccgccgg cgatcccgcc gccctagtgc cagcggtcgg
      241 gcgggatccg ctcgtcgggg tcgatgaact ccaggaagcg caggccgatc gcccgcgcgc
      301 tcgcggcgtc gttgccgccg gtgaagttga ggtaatggtg gtgcttgacc tcggcgcgga
      361 ccaccagctc gtcgatccca ttgggggctc tgccccccgt ctcgctgcgc tcgagcgggt
      421 cgtcgtagcg cacgtgctgg aagtgcacgg tgaccacggt gcccagcggc ggggcatagg
      481 tcatctcgac caggatcccg ccggccgaga tgttgcgggc gaccgcggtc gtgtcgccgt
      541 agagctcgct gccgatcacg accaggaacg ccttatcgaa gcgggtatgg atgcgcttct
      601 cgatgatcgg gaccgggatc gcggaagggg agcggtcctc caggacatgg gcgaggttca
      661 agggctcacg acgcttcacc gcatcatcat gaaaggcggc gccgatggag tcaaagaact
      721 taccttatcc tacataaagc acaaacgggt ctggctcccc ggccggcgat caggcagatt
      781 ctcggcgtgc gcagggtctg cctcatgctg tgcgttgtcg cgtcgaccgc ggtggcgtcg
      841 atcgcggtcg cgtcgccgcg tgcgagcaat ccggcgttcc tcgggatcca gatgatcgac
      901 gcctcgggcg gccgcgggcc gtgcgtgatc gagggcgcga cccgcgacgg tcccgccgag
      961 gcggccgggc tgcgcagcgg cgaccgcgtg ctcaccgtcg acggcaagcg gatcgccaac
     1021 tgcagcgcgc tgctcgacga gatcacggcg cacgcgccgg gtgagaccgt cgaggtcaag
     1081 gtcgagcggc tcggccgggc gctggtggtc aaggcccagc tgaccacgcg cgatgcgctg
     1141 ctccaccaca tgatcggcaa gccgatgatc gcgaccgacc tggtcggcgt cgaggacggc
     1201 gagacctacg acctgtcggc gctgcacggc cagatggcga tcgtcgggct gtacaacccg
     1261 gcgtgcgtcg actgcgcgtc gctgttcggc aagttcctcg actgggcgag cgaccggtcg
     1321 cggcgcgagc agccgccgct ggtgctcgcg gtcgttccgt cggccgagcc gacccacgac
     1381 ctgcgcgcgc tgcagaagtc gctcgacgtc ccgctcgcgg tcggcgagtt ctcgcccggc
     1441 ggcttcggcg gctcgccgtt cgcgcgcgac atgctgatct cggatcgcga ccggctcggc
     1501 gtgatcgtga tcgacggccg cggcaccgtg cagtacctcg ggccggtcgc ccccaacagc
     1561 gacgacaccg aggcggtact ggatgaggtg ttcgccgcgg ccgagctggc gtggcggcgc
     1621 tcgcggtaga tcgcgcatga cggcggtcat ccgcagcttc ggcggcaagc gtccgcagat
     1681 cggacgcggc gtgttcctcg cagagacctg tgcgctgatc ggcgacgtgg tgatcggcga
     1741 cgagtcgtcg atctggtacg gcgtggtgct gcgcggcgac atgtatccga tccgggtcgg
     1801 cgcgcgcacg aacctgcagg acggcaccat cgtccacatc accggcgggc agttcgcgac
     1861 cacgatcggc gacgactgca cggtgggaca cggcgcgatc gtgcacggat gtacgatcga
     1921 agatcactgc ctgatcggga tgggcgcgac gatcctcgac ggagcgcgcg tcggccgcgg
     1981 cagcctcgtg ggtgcaggtg cgctggtcac gccgggaacc gacatcccgc cgaacagcct
     2041 ggtgctggga gctcccgcca aggtcgtgag gtcggtccat ccgaaggaga cagagcagat
     2101 cgagtacggc gctgtgcact atgtcgagct ggcccgcagg taccttgcgg gcgacgccga
     2161 ttcctcggta ccgtagatcg tcgagtgaag cacggccgcg attcgacgtc tgactggacg
     2221 ggccaggccg gccggaggca acccggggga tgacttcgtc acgtgtgttc ccgatcgcgc
     2281 gcgccgcggt ccgcgtcgtc tcgatcgcag tggcgctcgt ggtggccggt cgcgccaccc
     2341 cggcgcgcgc cgacaacgtc gacaagctgg tccaccagct cggcgatgac gactccgaca
     2401 aggtccggct gtcggcggcg ctcaacctga ccaagctcgg cgatgcgcgc gtgagcgtga
     2461 tctcggcgct gaccaaggcg ctgggcaacg acaccgacga gggggtgcgc ggcgcatcgg
     2521 cggtcgggct cggcaagctg gtcaccgaca agaccgctcc gaaggtccgc aacgcggcgg
     2581 tcgacgccct cacccaggcc agccagtccg atcccagcgc cttcgtgcgc ggcgaggccg
     2641 gcaaggcgct caaggcgatc ggggtcacag cgcaggtcca gactccgcag gcggggaccg
     2701 cgatctacgt aaatatcggg ccgatggccg ccaagaccgg cgacccggcg gtcgatgccc
     2761 ggctcaaggc cttgatggtc aaggtcgcga accagacgat gacccaggtc gcgagtacca
     2821 tggcgatcac gtggccgggt ggggcgccgc ccacgcaggc ggcgctggcg gcgaagtcga
     2881 ccatcgggtt ctatgtcgat ggcacggtca atgagctcaa gatgaaacca gccggatcat
     2941 cgacgaccgt ttcttgtaag atcaacatgt tgctcgcaga ctacccgaac aagagcatct
     3001 tcgggctgct caccggcagc gcctcagtgg aagccagcgg ctccgcgagc agtctggcgc
     3061 tggcgggcga ggattgtgtg tccaagctgg tcgagaactt gatcgcgacg aagattgttc
     3121 cgacgatcaa cagcaaggtg ggaactccgc cgccgccgtc gaccagcagc aaggtgggaa
     3181 aaacgccgtg acgaaaccag gcgaccgcgg ctacaagcgg agctggaaga acctcttgat
     3241 caacaagcgg taccagctgc gcttcacctt gttcatggtc gggctcagcg cgctgctcat
     3301 ggtcggcctc ggggcgtggg tgatgtgggt cgccgacgag accacggcgg tgtcgatcac
     3361 cagcgtgcgg ggcgacgcct gccccaagct gccggcggtc aacgcgaccg gcgcgacctc
     3421 ggaggacgac ccggcggtgc acgcgccgtc gatgaagctc gacgaggccg atggggcggc
     3481 cgcgccggct cccggcagcg cggcgggctc ggccgcggac aacgaggagc cgcgccacgt
     3541 caaggtccag atcgacgaga gctcgatgac gctgaccccg acgctgccgc ccgacttcgc
     3601 cgatcgcgtc accgcgcact gggcctgcga gctgcgccag gccgcgacgt tcgatcggct
     3661 ggagcgcggc cggctgcgca tcctgtgggc gatgatcgcg accggcatcg cgctggtgat
     3721 cgggctggcg ctgtacggca tcaagatgac ccaccgggtc gccggtccgc tgttcaaggt
     3781 cggcctctac ctcagcaaga tgcgcgacgg ccggttcgac aaggtctaca acctgcgcaa
     3841 gggcgaccag ctggtcgact tctacgatca gttcaaggcc gcgcacggcg ggatcgtcgc
     3901 gatgcagcag gacgacatcg cgcggatcgc gcaggtgatc gcggcggccg acgccgccgg
     3961 cctcggccag caccccgtgg tcgccgagct ccgcgcgctg tccgcgcgga aggagaagtc
     4021 cattgagtga tccgaccgcc gccacggggc tcccatcgcc ggtgccgcgc tccaagcacc
     4081 agcgcaagct gtcgaactac ctgctcgaca agaacctgca gcttcgttac atcctgctgg
     4141 tcacgctgct gtcggcggcg atctcgggcg cgctcggcta catgatctac gaccagagcc
     4201 ggctggcgtc ggagtcgatc gagcgcgatc tgcaggtgct gaccgtcggc gacaccagcc
     4261 gccaggagtt caccagcatc ctcgccggca aggaccagat gctgatctac gagatggtcg
     4321 cgctcggcct cgggctggtc gtcatcctgt cggcctacct cgtgatcatg acccacaagg
     4381 tcgccgggcc gctgttcaag acgtcgatct acttcgatcg gatggccgag ggccggctca
     4441 gcgcggtcac gccgctgcgc cgcggcgaca tgctgcagga cttcttcggc aacttcaaag
     4501 cgatgcacga atcgctgcgc gtgcgcgccg agggcgacgt cgagatcatg gagcgcgcgg
     4561 cggcggcgat gcgcaaggcc caggacgagg ccgactaccg
//
LOCUS       DASVOR010000299         4203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_470385,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000299 DASVOR010000000
VERSION     DASVOR010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470385"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1223)
                     /locus_tag="VF516_06280"
     CDS             complement(165..1223)
                     /locus_tag="VF516_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8107317.1"
                     /translation="MLPAEIAQAVKRIQFVTGRQVSDVMAGAYLSVFKGRGMEFDEVR
                     PYVPGDDVRAIDWNVTARTGEPHVKRFVEERELTVMLLVDVSASQDFGSGRRSKLEAA
                     VELSALLAMSAVENGDKVGLLLFHGGADLYIPPRKGGKHALRVVREVLARGEARAEPI
                     DSGPRRSEDRNHLTARAPLVGGAPSGPSRRALFELPGAVRRWFARLAAKTRDEPRRST
                     SIAAALEFCRQVLPRRAVLFLISDFLDDGYLQVLRHANRKHDVVAALITDPREAEMPP
                     AGLVTLEDAETGQLRLVDTRSASFRSELAARARNRQDALRDQLRATGMDLVVFDASGS
                     MVDPLLRFFRERERRLRR"
     gene            complement(1243..2226)
                     /locus_tag="VF516_06285"
     CDS             complement(1243..2226)
                     /locus_tag="VF516_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8107318.1"
                     /translation="MSDRLTEDVTQFQGRFAALKAEVGKVLVGQDDMLHRLVLGLLAG
                     GHILLEGVPGLAKTLVIRTLADAVDGTFSRIQFTPDMLPGDVVGTQIFNPREGTYTVR
                     KGPVFGNLILADEINRAPAKVQSALLEAMQEKQVTLGDQTFRLAEPFLVLATQNPIEQ
                     EGTYPLPEAQLDRFMLKVKVGYPSRDDEVKIIDRMAGAGTEPTASKVMTTDEILAARE
                     VTRQVFVDDKVKRYAVDLVGATRDPRAANLASLAPLIDNGASVRASIALIKVAKAQAL
                     LSGRSYISPHDVKTIAHDVLRHRILVSYEAEAQGKTTDHVIAQILENVPVP"
     gene            2548..2859
                     /locus_tag="VF516_06290"
     CDS             2548..2859
                     /locus_tag="VF516_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8107319.1"
                     /translation="MVASEVGLARTFPRAKDPQLPSADQMSRRIRDEVGDVASAEVRL
                     CVGPDGRVQRVQVLRGSSSWAFDEALVRDMSDWQFPEAPGATTWQREKCEIAKVSYRV
                     H"
     gene            3190..>4203
                     /locus_tag="VF516_06295"
     CDS             3190..>4203
                     /locus_tag="VF516_06295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8107320.1"
                     /translation="MTWIERPGRELLRLAWPITVSMVSFSMMTLTSTAFVAHIGADEL
                     AGVGLAGVVGFALVCFGIGLLRGAKTLVSQAVGADRRDRIPELLAAALALAVALGAAA
                     MVLGHLIAPLLVSLSASPRAGRFAAQYLTIRSAGAPLVLVFAALREGRYGQGDTRAPM
                     RASLAGNAVNFALDATLILGLGWGVRGAAVATICGNLTELALLAWPMRSSLRGLTWRR
                     AAVREVWAQGLPNGAQFIMEVGSFLILTVLVASMSAIDGAAHQMVLHLVNVSFLPAHA
                     LAEAAAVLVGQAVGANRDALILRVARRTFLIGAGYAVVCGVVYAVLGGLITRALASGN
                     AALA"
BASE COUNT          545 a         1474 c         1524 g          660 t
ORIGIN      
        1 gaccggcgcc gcgctggccg cgggatcgcc ggcgtcgtcg tcgtggcagc cgcccgccgc
       61 ggcgagcgtg gccgcgaagc cggccaggca agccaggcag gccaggcgag ccgagcgaat
      121 gagatgcccg agccgcgcgt gggctcgcag cacagagcga tcggtcatcg gcgcagccgc
      181 ctttcgcgtt cgcggaagaa ccgcagcagg gggtcgacca tcgagccgga cgcgtcgaac
      241 acgaccagat ccatgccggt cgcgcgcagc tggtcgcgga gggcgtcctg gcggttgcgc
      301 gcgcgcgccg cgagctcgct gcggaagctc gccgaccggg tgtcgaccag ccggagctgg
      361 ccggtctccg cgtcctcgag cgtgaccagc ccggcgggcg gcatctcggc ctcgcgcgga
      421 tcggtgatca gcgccgcgac cacgtcgtgc ttgcggttgg cgtggcgcag cacctgcagg
      481 tagccgtcgt cgaggaagtc cgagatcagg aatagcaccg cgcggcgcgg caggacctgg
      541 cggcagaact ccagcgccgc ggcgatgctg gtcgaccgcc gcggctcgtc gcgggtcttc
      601 gcggcgagcc gggcgaacca ccggcgcacc gcgccgggca attcgaacag cgccctgcgc
      661 gacggccccg acggtgcgcc tcccacaagg ggggccctcg ccgtgaggtg attccgatcc
      721 tcgcttcgcc gcggaccgga atcgatcggt tcggctcggg cctcgccgcg cgcgagcacc
      781 tcgcggacca cgcgcagcgc gtgcttgccg cccttgcgcg gcgggatgta gaggtcggcg
      841 ccgccgtgga acagcagcag gccgaccttg tcgccgttct cgaccgcgct catcgcgagc
      901 agcgccgaca gctcgaccgc ggcctcgagc ttgctgcgcc ggcccgagcc gaagtcctgc
      961 gacgcgctga cgtcgaccag cagcatcacg gtcagctcgc gctcctcgac gaaccgcttg
     1021 acgtgcggct cgccggtccg cgcggtgacg ttccagtcga tcgcgcgcac gtcgtcgccg
     1081 gggacgtacg ggcggacctc gtcgaactcc atgccgcggc ccttgaacac cgacaggtac
     1141 gcgccggcca tgacgtcgga cacctggcgg ccggtgacga actggatgcg cttgacggct
     1201 tgcgcgatct ctgcgggcaa catggcgaac cttcgggcgc gcttacggga ccggcacgtt
     1261 ctcgaggatc tgcgcgatca cgtgatccgt ggtcttgccc tgggcctcgg cctcgtacga
     1321 caccaggatg cggtggcgca gcacgtcgtg ggcgatggtc ttgacgtcgt gcggggagat
     1381 gtagctccgg cccgacagca gcgcctgggc cttggcgacc ttgatcagcg cgatcgacgc
     1441 gcgcaccgac gcgccgttgt cgatcagcgg cgccaggctg gccaggttcg cggccctggg
     1501 atcgcgggtc gcgccgacca ggtcgaccgc gtagcgcttg accttgtcgt cgacgaacac
     1561 ctggcgcgtc acctcgcgcg ccgcgaggat ctcgtcggtg gtcatcacct tgctcgcggt
     1621 cggctcggtg cccgcgccgg ccatccggtc gatgatcttg acctcgtcgt cgcgcgacgg
     1681 gtagccgacc ttgaccttga gcatgaagcg atcgagctgg gcctcgggca aggggtaggt
     1741 gccctcctgc tcgatcgggt tctgggtcgc gagcacgagg aacggctcgg cgagccggaa
     1801 cgtctggtcg ccgagcgtga cctgcttctc ctgcatcgcc tcgagcagcg ccgactggac
     1861 cttggccggc gcgcggttga tctcgtcggc gaggatcagg ttgccgaaca ccgggccctt
     1921 gcggaccgtg taggtgccct cgcgcgggtt gaagatctgc gtgccgacca cgtcgcccgg
     1981 cagcatgtcg ggcgtgaact ggatccgcga gaacgtgccg tcgacggcgt cggccagggt
     2041 gcggatcacc agcgtcttgg cgaggccggg cacaccttcg agcaggatgt ggccgccggc
     2101 gagcagcccg agcacgaggc gatgcagcat gtcgtcctgg ccgaccagga ccttgccgac
     2161 ctcggccttg agcgctgcga accggccctg gaactgggtg acgtcttcag tgaggcgatc
     2221 ggacatggtg tggaccttgc gggggcggag caaaccgccc ggctagcggg ctgtcaacca
     2281 cccggacgcc ggctgcattc cccggggctg cgacatcgcg cgacgcctgc atgggacgtc
     2341 gcaaccgtgc tcgggcgcac gcaatttcgg cggcttggcg cgcgatcgcg aggccgaatc
     2401 gttggcacgc cgcgtggaac gcgcagactc acgatgggga ggacggggaa gaccatgatg
     2461 tcgaagtttg cggctgcgtc gtgtctggcg cttgccgcgt gtgccacggg caccgcggtg
     2521 cgcggggatc gggagggtgt ggcgccggtg gtggccagcg aggtcggcct cgcgcggacg
     2581 tttccgcggg ccaaggatcc gcagctgccg agcgccgatc agatgtcgcg ccggatccgc
     2641 gacgaggtcg gcgacgtcgc ctccgccgag gtccggctgt gcgtcggccc cgatggccgc
     2701 gtccagcgcg tccaggtgct gcgcggatcg tcgtcgtggg cgttcgacga ggcgctcgtg
     2761 cgcgatatgt ccgactggca atttccggaa gctcccggtg cgactacatg gcaacgggag
     2821 aagtgcgaga ttgccaaggt cagctatcga gttcactagc gagcacgaca gaagatcttg
     2881 acgtcgcgcc ggtcctgcgc gatggtgact gacacggaga tactcccatg agcagcgccc
     2941 tcttgccgag caagtgccag ccgacgcgcg accgtgtggt cggcgtcgac gatggcgctg
     3001 cccgcggcgg gcgaggctcg tgtagctgac acgagcgcaa ccgtgaccgc cgcgggctcc
     3061 ccggtcgact gaccccggag cccgtttcgt tttcagcgac gaccggactc cacggcatct
     3121 ctgttgcagc cataccgcgg gtatggcgtc gaggcctgta tgtccttgcc tgctagatct
     3181 acttcgtcgt tgacctggat cgaacgcccc ggccgcgagc tgctccgcct cgcctggccg
     3241 atcacggtgt cgatggtctc gttctcgatg atgacgctga cctcgaccgc gttcgtcgcg
     3301 cacatcggcg ccgacgagct cgccggcgtc gggctcgccg gcgtcgtcgg gttcgcgctg
     3361 gtgtgcttcg ggatcggcct tctgcgcggg gccaagaccc tggtgtcgca agcggtcggc
     3421 gcggatcgcc gcgatcgcat ccccgagctg ctcgccgccg cgctcgccct cgccgtcgcg
     3481 ctcggcgcgg ccgcgatggt gctcggccac ctgatcgcgc cgctgctcgt gtccctgtcc
     3541 gcgtcgcccc gcgccggccg gttcgccgcc cagtacctca cgatccgctc ggccggtgcg
     3601 ccgctcgtgc tggtgttcgc cgcgctgcgc gaggggcgct acggccaggg cgacacccgg
     3661 gcgccgatgc gcgcgtcgct cgccggcaat gccgtcaact tcgcgctcga cgcgacgctg
     3721 atcctcggcc tgggctgggg cgtccgcggc gcggcggtcg ccacgatctg cggcaacctc
     3781 accgagctcg cgctgctcgc gtggccgatg cgatcctcgc tgcgcggcct gacctggcgc
     3841 cgcgccgcgg tgcgcgaggt ctgggcccag ggcctgccca acggggcgca gttcatcatg
     3901 gaggtcggct cgttcctgat cctcaccgtg ctggtcgcat cgatgtcggc gatcgacggc
     3961 gccgcgcacc agatggtcct ccacctggtc aacgtgtcgt tcctccccgc gcacgcgctc
     4021 gccgaggcgg cggccgtgct cgtcggccag gcggtcggcg ccaaccggga cgcgctgatc
     4081 ctgcgcgtcg cgcgccgcac gttcctgatc ggcgccggct acgcggtcgt gtgcggcgtg
     4141 gtctacgccg tgctcggcgg cctgatcacc cgggcgctcg cgtcgggcaa cgccgcgctc
     4201 gcc
//
LOCUS       DASVOR010000300         2724 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_236426,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000300 DASVOR010000000
VERSION     DASVOR010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236426"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1638
                     /locus_tag="VF516_06300"
     CDS             <1..1638
                     /locus_tag="VF516_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8107321.1"
                     /translation="TFTTLPAAAQPRGRSQANSASAPAAPAAPADDDGDGPEAKAVAT
                     FRLNDIINVAVRLSPELARAVTSRDAARQDAIAATKDQSWRLTASANVEVDALGADTP
                     SDAIAPLTPLSTQKISGSLGLGRKLPTGGEIGVGVSLTGQHQELNVTGEALAQQPAPM
                     QLQSQCGEKADVFCQVQASASVTFKQPLLKGLGSDVALAGEHKAEIAAAKATVESQLA
                     AEKMILDLVTAYWELANASYEVDVRAEALDLARKQEQLTRQEIRSGTSEANSLDAVTY
                     EIAVREEALLTAKLQFEQKSLDLRRKAGLEIGRRDIVVRPADPLELDAQEDWSIDPIL
                     AQSHKINRQLTTIVLDKKTADIEVDVTRNATLPQVDLSLQGALLATGDTTADAFDGLS
                     GNSNSGGFGYQVMAGLTMSFELSGAAKAAHAAAVSRRHLLDIQRVDKERELDAQVVSQ
                     VKAVMSDRTRVALADKAIAIAENNVKAERAKFLAHAPNSTNFQVMQVQTKLIEARLRR
                     GQAVAAYRTAVAQLQFLSGTLLDTYRIHVRARGGAAE"
     gene            1755..>2724
                     /locus_tag="VF516_06305"
     CDS             1755..>2724
                     /locus_tag="VF516_06305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107322.1"
                     /translation="MRLTTLSLVVSLGVLSSLAGACLGTTGDPRKPRGAGSGSDQTAA
                     DRPCTKVESDVTIRSPADMSSLPRTGCYDLYGKLTLQGQTITSLAGLGEINSVNGLDL
                     DHTAIATIDTRRPLGIYGNLTVTGNAKLTNLKQLSFETASTGILIDSNPALTSLDALG
                     LADPQLQEVDGDIVITGNTALTAIPLGNLTKLTGALTVSGNSALTSIALDKLAGAGHV
                     EISDNAKLTTLGAMNALYRVTGSVTIDGNPALTGLAAFTTSLKFIDQTLTISNNAALT
                     DLGALKHVALIGAISITNNTSLVSCRATEVDKCTQHPIASVISNNKT"
BASE COUNT          481 a         1044 c          868 g          331 t
ORIGIN      
        1 accttcacca cgctcccggc ggccgcccag ccgcgtggtc ggtcgcaggc gaactcggcg
       61 tccgcgccgg ccgcgccggc cgcgcccgcg gacgatgacg gcgatggccc cgaggccaag
      121 gcggtcgcga cgttcaggct caacgacatc atcaatgttg cggtccggct gtctccggag
      181 ctcgcccgcg cggtgaccag ccgcgacgcc gcgcggcagg acgcgatcgc cgccaccaag
      241 gatcagtcgt ggcggctgac cgccagcgcc aacgtcgagg tcgacgcgct cggcgccgac
      301 acccccagcg acgcgatcgc gccgctgacg ccgctgtcga cccagaagat cagcggctcg
      361 ctcggcctgg gccgcaagct gccgaccggc ggcgagatcg gcgtcggggt cagcctcacc
      421 ggacagcacc aggagctcaa cgtgaccggc gaggcgctgg cccagcagcc ggcgccgatg
      481 cagctgcaga gccagtgcgg cgagaaggcc gacgtgttct gccaggtcca ggccagcgcg
      541 agcgtgacct tcaagcagcc gctgctcaag ggcctcggct cggacgtcgc gctcgccggc
      601 gagcacaagg ccgagatcgc cgccgccaag gcgaccgtgg agtcccagct cgccgccgag
      661 aagatgatcc tcgatctggt caccgcgtac tgggagctcg ccaacgcgtc ctacgaggtg
      721 gacgtccgcg ccgaggcgct cgacctcgcc cgcaagcagg agcagctgac ccgccaggag
      781 atccgctcgg gtacgtcgga ggccaactcg ctcgacgcgg tgacctacga gatcgcggtg
      841 cgcgaggagg cgctgctgac cgccaagctg cagttcgagc agaagtcgct ggatctgcgc
      901 cgcaaggccg gcctcgagat cggccggcgc gacatcgtgg tccggcccgc cgatccgctc
      961 gagctcgacg cccaggagga ctggagcatc gacccgatcc tcgcgcagag ccacaagatc
     1021 aaccgccagc tcacgacgat cgtgctcgac aagaagaccg ccgacatcga ggtcgacgtc
     1081 acccgcaacg cgacgctgcc ccaggtcgat ctcagcctgc agggcgcgct gctcgcgacc
     1141 ggcgacacca ccgccgacgc gttcgacggc ctgagcggca acagcaacag cggcgggttc
     1201 ggctaccagg tgatggccgg cctgacgatg tcgttcgagc tgtcgggcgc ggcgaaggcg
     1261 gcgcacgcgg ccgcggtctc ccgccgccac ctgctcgaca tccagcgcgt cgacaaggag
     1321 cgcgagctcg acgcccaggt cgtgtcgcag gtcaaggcgg tgatgagcga tcggacccgg
     1381 gtcgcgctgg ccgacaaggc gatcgcgatc gccgagaaca acgtcaaggc cgagcgcgcc
     1441 aagttcctcg cccacgcccc gaattcgacc aacttccagg tcatgcaggt ccagaccaag
     1501 ctgatcgagg cccggctgcg ccgcggccag gcggtcgccg cctaccgcac cgcggtcgcc
     1561 cagctgcagt tcctgagcgg cacgctgctc gatacctaca ggattcacgt ccgcgcgcgc
     1621 ggcggggccg cggagtagcg atgcgtcccc gggttgacat cgtgcggacc ccgaccccga
     1681 tccgatcgac tgcaaccgcg ggtagtcacg caacgagccc tctggcacgc accctgctcc
     1741 ctctggagat gcccatgcga ctcaccaccc tcagcctcgt tgtcagcctc ggtgtcctgt
     1801 ccagcctcgc cggggcctgc ctcggcacga ccggcgaccc ccgcaagccg cgcggcgcgg
     1861 gctcgggctc ggaccagacc gccgccgacc gcccgtgcac caaggtcgag agcgacgtga
     1921 ccatccgcag ccccgccgac atgtcctcgc tgccccgcac cggctgctac gacctctacg
     1981 gcaagctgac gctgcagggc cagacgatca ccagcctggc cgggctgggc gagatcaact
     2041 cggtcaacgg gctcgacctc gaccacaccg cgatcgcgac gatcgacacc aggcggccgc
     2101 tcggcatcta cggcaacctg acggtgaccg gcaacgccaa gctgaccaac ctcaagcagc
     2161 tgtcgttcga gaccgcctcg accggcatcc tgatcgacag caaccccgcg ctgacctcgc
     2221 tcgacgcgct cgggctcgcc gacccccagc tccaggaggt cgacggcgac atcgtgatca
     2281 ccggcaacac cgcgctgacc gcgatcccgc tgggcaacct gaccaagctg accggcgcgc
     2341 tcaccgtgtc gggcaactcg gcgctgacct cgatcgcgct cgacaagctc gccggcgccg
     2401 gccacgtcga gatctccgac aacgccaagc tcaccacgct cggcgcgatg aacgcgctgt
     2461 accgggtgac cggcagcgtg acgatcgacg gcaaccccgc gctcaccggc ctcgcggcgt
     2521 tcaccaccag cctcaagttc atcgaccaga ccctgacgat cagcaacaac gcggcgctca
     2581 ccgacctcgg cgcgctcaag cacgtcgcgc tgatcggcgc gatcagcatc accaacaaca
     2641 ccagcctggt gagctgccgc gccaccgagg tcgacaagtg cacccagcac ccgatcgcct
     2701 cggtcatctc caacaacaag acca
//
LOCUS       DASVOR010000301         2979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_2852,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000301 DASVOR010000000
VERSION     DASVOR010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2979
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2852"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF516_06310"
     CDS             complement(<1..321)
                     /locus_tag="VF516_06310"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8107323.1"
                     /translation="MLRPTLKADDFLVAASPAMRAVTAAVERYADGDSPVLICGEHGT
                     GRELVARVLHRRGPDRCSTRAISSRPVPCSPQIRTGTSPSAYAPTRLTTCFIAGLSAT
                     RNVLA"
     gene            complement(322..1500)
                     /gene="dusB"
                     /locus_tag="VF516_06315"
     CDS             complement(322..1500)
                     /gene="dusB"
                     /locus_tag="VF516_06315"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEX8107324.1"
                     /translation="MRIGDLGVDVPVLLAPMAAVTDLPFRTVCEEFGVGLTITEFLSA
                     HALSIGDPRTCGKLTASLDGRRFGVQIFGREPAAMDAAARLAVAIGASLVDINMGCPA
                     KRVVAGECGSALMREPAIAQELVRAVVAAVPPDVPVTVKHRAGWSQDHRNAPEFACAM
                     VEAGAKMITVHGRTRTQGFSGKSDPDIIQQVRDAVPSHIPVVGNGDVVDVAGYLRLRE
                     HTGCDAVMIGRGALGNPWLFARLREVTAGRPDPGPPGLDERLAVFRRHVDLIEALKRG
                     PRMLHEVRKSCAWYARGLYGCNALRLRVWEVPDVAAARALVEDYFAQLIERQRRLGLA
                     PEGERGDMDGAFAGTETATETATETATETATETTTETTTETTTETTTETTTETTDEAA
                     "
     gene            complement(1781..>2979)
                     /locus_tag="VF516_06320"
     CDS             complement(1781..>2979)
                     /locus_tag="VF516_06320"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEX8107325.1"
                     /translation="PIPRRVGLADVPAVQGLLAVQRLDGWLLFDRDGENPIAERLVAP
                     DGHPTRSWFYMIPARGTPIALVHSTELRSFDHLPGTKLTYQGYRDLDKQLRAMLKGIK
                     AVAVEYSAKAAVPAVSRVDAGTLEVIRAAGVQVRSSDTLVQFTKAIWGDAGRTAHYVA
                     VHHVVELRKEALAFLAKRLQSGEPVTEHDVQKEILRGMTMRGLVGPPPVVAAGANTAD
                     PYYVPSADKAAPIKRGDPIVISLAAKLDQPEGIYAAQTWCAVADQAVPDPIARAFETV
                     SLARDQAMALISDRARKHRPVTGAEVDDATRAFLRKAGLADRVMHRTGHSIDNDLQGG
                     GADLDDFEVKDSRILTPGTGFTIGPGVYFTGQFGVRSEVSVYLAPGGPEVTTPAQDEV
                     EALLKP"
BASE COUNT          409 a         1104 c         1045 g          421 t
ORIGIN      
        1 cgccaagacg ttcctggtcg ccgacagccc cgcgatgaag caggtcgtga gccgggtcgg
       61 cgcgtacgcc gacggcgacg tgccggtcct gatctgcggc gagcacggca ccggccgcga
      121 gctgatcgcg cgcgtgctgc acctgtcggg gccgcgccgg tgcaggaccc gcgcgaccag
      181 ctcgcggccg gtgccgtgct cgccgcagat cagcaccggg gaatcgccgt cggcgtagcg
      241 ctcgaccgcg gcggtcaccg cgcgcatcgc cgggctcgcg gcgaccagga agtcgtccgc
      301 cttgagcgtg gggcggagca tctacgcggc ctcgtccgtc gtctcggtcg tcgtctcggt
      361 cgtcgtctcg gtcgtcgtct cggtcgtcgt ctcggtcgtc gtctcggtcg ccgtctcggt
      421 cgccgtctcg gtcgccgtct cggtcgccgt ctcggtgccg gcgaacgcgc cgtccatgtc
      481 gccgcgctcg ccctcgggcg cgaggccgag ccggcgctgg cgctcgatca gctgcgcgaa
      541 gtagtcctcg accagcgcgc gcgccgccgc gacgtccggc acctcccaca cccgcagccg
      601 gagcgcgttg cagccgtaca ggccgcgcgc gtaccacgcg cacgacttgc ggacctcgtg
      661 cagcatccgc ggtccgcgct tgagcgcctc gatcagatcg acgtggcggc ggaacaccgc
      721 gagccgctcg tcgagcccgg gcggtccggg atccggccgg cccgcggtga cctcgcgcag
      781 ccgcgcgaac agccacgggt tgccgagcgc gccgcggccg atcatcaccg cgtcgcagcc
      841 ggtgtgctcg cgcagccgca ggtagcccgc gacgtcgacg acgtcgccgt tgccgaccac
      901 cgggatgtgg gacggcacgg cgtcgcgcac ctgctggatg atgtccggat cactcttgcc
      961 ggagaacccc tgggtgcggg tccggccgtg caccgtgatc atctttgcgc cggcctcgac
     1021 catcgcgcac gcgaactcgg gcgcgttgcg gtgatcctgg ctccatccgg cgcgatgctt
     1081 gacggtgacc ggcacgtccg gcggcaccgc ggcgaccacc gcgcgcacca gctcctgcgc
     1141 gatcgcgggc tcccgcatca gcgccgagcc acactcaccg gcgaccacgc gcttggccgg
     1201 gcagcccatg ttgatgtcga ccagcgacgc gccgatcgcg accgcaagac gcgccgcggc
     1261 gtccatcgcc gccggctccc ggccgaagat ctggaccccg aaccgccggc cgtcgagcga
     1321 ggccgtgagc ttgccgcagg tcctgggatc gccgatcgac agcgcatgtg ccgacagaaa
     1381 ctcggtgatc gtgagcccga cgccgaactc ctcgcacacc gtccgaaatg gcagatccgt
     1441 gaccgccgcc atcggtgcca ggagcaccgg gacgtcgacg ccgagatcac cgatacgcat
     1501 caccggcacc tatacaggaa tcgggttggc atgagaaccc ggaaaaccgc ggcgaacgtg
     1561 gccgggaacg tgatcgacca ccgcgagccg cggcgcctcg cgtgtgatca cgcagtgtca
     1621 cggtgacatg tcctccggcg catggacgtt gtgcagcgcg cgcaggcccg gatcgaccgc
     1681 gcgcagctcg tcctcctcga tccaacgtac ccgcagcggc gagcctcccg accaccaccg
     1741 cgcgccacgc tgcagtgcgc atcacggtgc acgacctgga tcaaggcttg agcagcgcct
     1801 cgacctcgtc ctgcgccggc gtcgtgacct ccggaccgcc gggcgctaga tagaccgaga
     1861 cctcgctgcg cacgccgaac tggccggtga agtacacgcc cggtccgatc gtgaagccgg
     1921 tgcccggcgt gaggatccgc gagtccttga cctcgaagtc gtcgagatcc gcgccgccgc
     1981 cctgcagatc gttgtcgatc gagtgccccg tgcggtgcat cacccggtcg gcgagcccgg
     2041 ccttgcggag aaacgcgcgg gtggcgtcgt cgacctcggc gccggtgacc gggcgatgct
     2101 tgcgcgcgcg gtcgctgatc agcgccatcg cctgatcgcg cgccaggctc acggtctcga
     2161 acgcccgcgc gatcggatcg ggcaccgcct ggtcggcgac cgcgcaccag gtctgcgccg
     2221 cgtagatgcc ctcgggctgg tcgagcttgg cggcgagcga gatcacgatc ggatcgccgc
     2281 gcttgatcgg cgccgccttg tcggccgacg gcacgtagta cgggtcggcg gtgttggcgc
     2341 cggcggcgac caccggcggc gggccgacca gcccgcgcat cgtcatcccg cgcaggatct
     2401 ccttctggac gtcgtgctcg gtgaccggct cgccgctctg cagccgcttg gccaggaacg
     2461 ccagcgcctc cttgcgcagc tcgaccacgt ggtggaccgc gacgtagtgc gcggtgcgcc
     2521 cggcgtcgcc ccagatcgcc ttggtgaact ggaccagcgt gtcggacgac cggacctgta
     2581 cgccggcggc gcggatcacc tcgagcgtac cggcatcgac ccgcgacacg gcgggcaccg
     2641 ccgccttggc cgagtactcg accgcgaccg ccttgatgcc cttgagcatc gcgcgcagct
     2701 gcttgtcgag atcgcggtag ccctggtagg tcagcttggt gccggggagg tgatcgaagc
     2761 tgcgcagctc ggtggagtgg accagcgcga tcggcgtgcc gcgcgccggg atcatgtaga
     2821 accacgaccg cgtcgggtgg ccgtcgggcg cgaccagccg ctcggcgatc gggttctcgc
     2881 cgtcgcgatc gaacagcagc cagccgtcga gccgctggac cgcgagcagg ccctgcacgg
     2941 cgggaacgtc ggcgagcccg acccgccgcg ggatcggcg
//
LOCUS       DASVOR010000302         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_31897,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000302 DASVOR010000000
VERSION     DASVOR010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31897"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..609)
                     /locus_tag="VF516_06325"
     CDS             complement(136..609)
                     /locus_tag="VF516_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107326.1"
                     /translation="MSTAPTLARSYEALRPRVLERVLELRATLDPSLGRLEDATARAQ
                     IGAVLDHLGNFLATGDLGLHRGFLHTFLAMRAAEAQSPASVLAMLVAIGDTAAQVAQE
                     DATGSAGSDGAELTLLLTRVTASTARAMNDLLAEELGRRAAQRAELARRAHEVEP"
     gene            677..1453
                     /locus_tag="VF516_06330"
     CDS             677..1453
                     /locus_tag="VF516_06330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107327.1"
                     /translation="MKEGRVLVVDDEPMVRDTLGQVLSEEGYVVDLAVDGETALERVR
                     AARPDAILLDLMMPGMNGRQFLQALRNDDAYKNVPVMIMTAVHGLELSATLGASEVVE
                     KPFNPDDLLNKVALAVYRSREAATPTQPGLPVLTVPPESTPEKERGVVLLCEHDRSRL
                     QQLDLLLSARGYTVVSMTRALTQLSRLARALRPRAILLDEVSEGVDDVVRELADSSVL
                     EQIPILVFSRAGASRIPGTLEQATDVDLVGFVEGQRPPTR"
BASE COUNT          206 a          570 c          562 g          212 t
ORIGIN      
        1 cgttggcccc gcccatgccg tcgagcacgg cgtacagccc gagccgcggc tcgaggatca
       61 gcgtgtcctc gttggtcgtc cggaccttgc cgatgtggct gtcgccggcg ctccgcatcg
      121 ccggcccagc gtgggtcacg gctcgacctc gtgcgcgcgg cgggcgagct cggcgcgctg
      181 cgcggcccgc cggccgagct cctccgcgag caggtcgttc atcgcgcgcg ccgtgctcgc
      241 cgtcacgcgg gtgagcagca gcgtcagctc ggcgccgtca ctcccggccg acccggtcgc
      301 gtcctcctgt gcgacctgcg ccgcggtgtc cccgatcgcg accagcatgg cgagcaccga
      361 ggccgggctc tgggcctcgg ccgcgcgcat cgccaggaag gtatgcagaa acccacgatg
      421 caggccgagg tcgccggtcg cgaggaagtt gccgaggtgg tcgagcaccg cgccgatctg
      481 tgcccgggcg gtcgcatcct cgaggcggcc gagcgacgga tccagcgtgg cgcgcagctc
      541 caggacccgc tccagcaccc gcgggcgcag cgcctcgtag gacctggcca gcgtcggcgc
      601 cgtcgacatt cgccgaatcg tacaccgcac ggtgcgccgg gtgatacgct tgccccgtgg
      661 aaaccggatg agtggaatga aagaagggcg tgtcctcgtc gtcgacgacg aacccatggt
      721 gcgcgacacc ctcggccagg tcctgagcga ggagggctac gtggtcgatc tcgcggtcga
      781 cggcgagacc gcgctggagc gggtccgcgc ggcccggccc gacgcgatcc tgctcgacct
      841 gatgatgccg ggcatgaacg gccgccagtt cctccaggcg ctgcgcaacg acgacgcgta
      901 caagaacgtg ccggtcatga tcatgaccgc ggtccacggc ctggagctca gcgcgacgct
      961 cggcgccagc gaggtggtcg agaagccgtt caaccccgac gacctgctca acaaggtcgc
     1021 gctcgcggtg tatcgctccc gcgaggccgc gaccccgacc cagcccgggc tgccggtgct
     1081 gacggtgcct ccggagtcca ctcccgagaa ggagcgcggc gtcgtgctgc tgtgcgagca
     1141 cgaccgcagc cggctccagc agctcgactt gctgctgtcg gcgcgcggct acaccgtggt
     1201 gtcgatgacc cgcgcgctga cccagctgtc gcggctggcc cgcgcgctcc ggccccgcgc
     1261 gatcctgctc gacgaggtca gcgagggcgt cgacgacgtc gtccgcgagc tcgccgacag
     1321 cagcgtgctg gagcagatcc cgatcctcgt gttctcgcgc gccggcgcgt cgcgcatccc
     1381 cggcacgctg gagcaggcga ccgacgtcga cctggtcggc ttcgtcgagg gccagcgccc
     1441 cccgacccgg tgatgccgag ggggttggcg cgtcatgcgc cggccgggcc ggggggcacc
     1501 ggctcgccga gccacgccgc cgccagggcg agcgtgcggg tgttctcggg
//
LOCUS       DASVOR010000303         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_324202,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000303 DASVOR010000000
VERSION     DASVOR010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324202"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2070
                     /gene="nuoL"
                     /locus_tag="VF516_06335"
     CDS             <1..2070
                     /gene="nuoL"
                     /locus_tag="VF516_06335"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEX8107328.1"
                     /translation="YMAHDPRYSAFFGYLNLFTGAMLILVLAANLPVMFIGWEGVGLC
                     SYLLIGFWFENESYATAGRKAFVVNRIGDFAFLLGMFLLFWATKNLNAGDGGSLDFAM
                     LRSAAAGPTYVQGFWGGERLAATAGILLFIGACGKSAQLPLFVWLPDAMAGPTPVSAL
                     IHAATMVTAGVYMVCRLSLLYASSTTALIVVSTVGLLTALAAAFMAFAQTDLKKVLAY
                     STVSQLGFMFVAAGTGNWTAAIFHLGTHAFFKACLFLDAGSVMHAMEHAGSETPGDIT
                     TMGGLRSRMKITHATFAISCLAIAGIPLFSGFFSKDEILAGAFAVHPPGWPALYGKIL
                     WAGLLIAALGTAFYMWRLYFLVFGSDARSEPARHAHESPSAMTLPLVVLAVGALLGGV
                     VGLPHFEHLHLPQWLGEWLKPSLTEEWYASGLPGPQGIEAHASDGTIWGLLSVASVIA
                     VVGVALAWLFYSRGPSKTVERWTSRRGVFADVYAASKAKLWVDEAYDVVIVGPFKVVA
                     RFLLEFVDRFVVDTVAVNGSAFVVGIFGRISRWFQNGNVQRYLVGVVVGGALVFVITD
                     CHHKATFDYKIVGNQIQLHADPGSGILGSTARVYWDLDGDGQKDHDPAHPDELLHDRD
                     VTVTGYDGPITMWVADPVTGREIKVTRTINLNEGRTFWPPPEPAADQHQPGAGRPATS
                     PLGQGGK"
     gene            2073..>3802
                     /locus_tag="VF516_06340"
     CDS             2073..>3802
                     /locus_tag="VF516_06340"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEX8107329.1"
                     /translation="MQDFTRGDFLIPLVLAVPVLGAIFVMCTPKTEAPLHRGIGLTFT
                     VITFFISLLMIGNFDPKFDGFQLKLDVEWIPVLGAHFKTGVDGMSIFLVILTTFLMPL
                     TLLGTGKAIEKNVREFIAAMLVLETGMVGAFVALDVFLFYVFWEVMLIPMYLLIGIWG
                     GQRRLYASIKFVIYTMVGSLLMLVAIFYVYVKAGAVLGSYTTDLEKLLTVSLPYGAQT
                     WCFAAFALAFAIKIPLFPLHTWLPDAHVEAPTAGSVILAGVLLKFGVYGFLRYAMPLF
                     PHGAVVWAPTIAVLSVIGIIYGAFVAFAQDDVKKLVAYSSVSHLGFCALGVVALTPYG
                     ISGSIFAMLSHGLTTGGLFLGIGVLYERRHTRRLAEYGGIWKQMPVFAGLFLIVTMGS
                     AGLPALSGFVGEFLTILGTFIAGDTLPQGYSIPSPRLLGALAATGVVLGAIYLLWMFQ
                     KVFFGKLDKARNGRLPDLNPRERFVFVVLAIAIFALGVKPRPLLAAMEPSVNKFIKDF
                     GRRVGECDGPPHTFGSGVCDNNPAIQKTLDSVARGAAGVQKTISGAGAGSAAGPGSAA
                     PGNPGAGTAAP"
BASE COUNT          497 a         1302 c         1305 g          698 t
ORIGIN      
        1 tacatggccc acgacccgcg ctacagcgcg ttcttcggct acctgaacct gttcaccggc
       61 gcgatgctga tcctggtgct ggccgccaac ctgccggtga tgttcatcgg ctgggagggc
      121 gtcggcctgt gctcgtacct cttgatcggg ttctggttcg agaacgagtc ctacgcgacc
      181 gcgggccgca aggcgttcgt ggtcaaccgg atcggcgact tcgcgttcct gctcggcatg
      241 ttcctcctgt tctgggcgac caagaacctc aacgccgggg acggcgggag cctggacttc
      301 gcgatgctgc gcagcgccgc ggccggcccg acctacgtcc aggggttctg gggcggtgag
      361 cggctggcgg cgaccgccgg gatcctgctg ttcatcggcg cgtgcggcaa gtcggcccag
      421 ctgccgctgt tcgtgtggtt gcccgacgcg atggccggtc cgacgccggt ctccgcgctg
      481 atccacgccg cgaccatggt gaccgccggc gtctacatgg tgtgccggct gtcgctgctc
      541 tacgcgtcgt cgaccacggc gctgatcgtg gtctcgacgg tcggtctgct gaccgcgctg
      601 gccgcggcgt tcatggcgtt cgcccagacc gatctcaaga aggtgctggc gtactccacg
      661 gtgtcgcagc tcggcttcat gttcgtcgcc gccggcaccg gcaactggac cgcggcgatc
      721 ttccacctcg gcacccacgc gttcttcaag gcctgcctgt tcctcgacgc cggctcggtg
      781 atgcacgcga tggagcacgc cggctcggag acgccgggcg acatcacgac gatgggcggg
      841 ctgcgcagcc ggatgaagat cacccacgcg acgttcgcga tctcgtgcct ggcgatcgcc
      901 ggcatcccgc tgttctccgg gttcttctcc aaggacgaga tcctcgccgg cgcgttcgcg
      961 gtccacccgc cgggctggcc ggcgctctac ggcaagatcc tgtgggcggg cctcctgatc
     1021 gccgcgctcg gcacggcatt ctacatgtgg aggctgtact tcctggtgtt cggcagcgat
     1081 gcgcgctccg agcccgcgcg ccacgcccac gagtcgccgt cggcgatgac gctgccgctg
     1141 gtcgtgctcg cggtcggcgc gctcctcggc ggcgtggtcg gcctgccgca cttcgagcac
     1201 ctccacctgc cgcagtggct cggcgagtgg ctgaagccga gcctgaccga ggagtggtac
     1261 gcgtccgggc tgcccgggcc gcagggcatc gaggcccatg cctcggacgg cacgatctgg
     1321 gggctgctgt cggtcgcgtc ggtgatcgcg gtggtcggcg tcgcgctggc ctggctgttc
     1381 tacagccgcg gcccgtcgaa gacggtcgag cgctggacct cccggcgggg cgtgttcgcc
     1441 gacgtctacg ccgcgtcgaa ggccaagctg tgggtcgacg aggcctacga cgtggtcatc
     1501 gtggggccgt tcaaggtggt cgcgcggttc ctgctcgagt tcgtcgaccg cttcgtggtc
     1561 gacaccgtcg cggtcaacgg ctcggcgttc gtggtcggca tcttcggccg gatctcgcgg
     1621 tggttccaga acggcaacgt ccagcgctac ctggtcggcg tggtggtcgg cggcgcgctg
     1681 gtgttcgtga tcaccgactg ccaccacaag gcgacgttcg actacaagat cgtcggcaac
     1741 cagatccagc tccacgcgga tcccgggtcc ggcatcctcg gctcgaccgc gcgggtctac
     1801 tgggacctcg acggcgacgg tcagaaggac cacgatccgg cgcacccgga cgagctgctc
     1861 cacgaccgcg acgtgacggt caccggctac gacggaccga tcacgatgtg ggtcgcggat
     1921 ccggtcaccg gccgcgagat caaggtcacc cggacgatca acctcaacga gggccgcacg
     1981 ttctggccgc ctcccgagcc cgcggctgat caacatcaac ccggcgccgg ccggccggcg
     2041 acgtctcctc tcgggcaggg ggggaaataa ccatgcagga cttcacacgc ggtgacttcc
     2101 tgatcccgct ggtcctcgcc gtgccggtgc tcggcgcgat cttcgtgatg tgcacgccca
     2161 agaccgaggc gccgctccac cgcggcatcg gcctgacgtt cacggtgatc acgttcttca
     2221 tctcgctgct gatgatcggc aacttcgatc cgaagttcga cggcttccag ctcaagctcg
     2281 acgtcgaatg gatcccggtg ctcggcgcgc acttcaagac cggcgtcgac gggatgtcga
     2341 tcttcctggt gatcctgacc accttcctga tgccgctgac cctgctcggc accggcaagg
     2401 cgatcgagaa gaacgtccgc gagttcatcg ccgcgatgct ggtgctcgag accggcatgg
     2461 tcggcgcgtt cgtcgcgctc gacgtgttcc tgttctacgt gttctgggag gtgatgctga
     2521 tcccgatgta cctcctgatc gggatctggg gcggtcagcg ccggctgtac gcgtcgatca
     2581 agttcgtgat ctacacgatg gtcggctcgc tgctgatgct ggtggcgatc ttctacgtct
     2641 acgtcaaggc cggcgcggtg ctcggcagct acaccacgga tctggagaag ctgctgaccg
     2701 tgtcgctgcc gtacggcgcg cagacctggt gcttcgcggc gttcgcgctc gcgttcgcga
     2761 tcaagatccc gctgttcccg ctccacacct ggttgcccga cgcgcacgtc gaggccccga
     2821 ccgccggctc ggtgatcctg gccggcgtgc tgctcaagtt cggggtctac ggcttcctgc
     2881 gctacgcgat gccgctgttc ccgcacggcg cggtggtatg ggcgcccacg atcgcggtgc
     2941 tctcggtgat cggcatcatc tacggcgcgt tcgtcgcgtt cgcccaggac gacgtcaaga
     3001 agctggtcgc gtactcgtcg gtttcgcacc tcgggttctg cgcgctcggc gtggtcgcgc
     3061 tgaccccgta cgggatctcg ggatcgatct tcgccatgct gtcgcacggc ctcaccaccg
     3121 gcgggctgtt cctcgggatc ggcgtcctgt acgagcgccg ccacacccgg cggctcgccg
     3181 agtacggcgg gatctggaag cagatgccgg tgttcgcggg gctgttcctg atcgtcacga
     3241 tgggctcggc ggggctcccg gcgctgtcgg gcttcgtcgg cgagttcctg accatcctgg
     3301 ggacgttcat cgccggcgac acgctgccgc agggctactc gatcccgtcg ccgcggctgc
     3361 tcggcgcgct ggccgcgacc ggcgtcgtgc tcggcgcgat ctacctgctc tggatgttcc
     3421 agaaggtgtt cttcggcaag ctcgacaagg cgcgcaacgg ccggctgccg gatctcaacc
     3481 cgcgcgagcg cttcgtgttc gtcgtgctgg cgatcgccat cttcgcgctc ggcgtgaagc
     3541 cccggccgct gctcgccgcg atggagccgt cggtgaacaa gttcatcaag gacttcggcc
     3601 gccgggtcgg cgagtgcgat ggcccgccgc acaccttcgg gtcgggcgtg tgcgacaaca
     3661 acccggcgat ccagaagacg ctcgacagcg tcgccagggg ggccgccggg gtgcagaaga
     3721 cgatctcggg cgccggggcg ggctcggccg cgggcccggg cagcgctgcg ccggggaacc
     3781 cgggtgcggg aaccgccgcg cc
//
LOCUS       DASVOR010000304         2284 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_2863,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000304 DASVOR010000000
VERSION     DASVOR010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2284
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2863"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..1876)
                     /locus_tag="VF516_06345"
     CDS             complement(269..1876)
                     /locus_tag="VF516_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107330.1"
                     /translation="MRRVAGVGWLLLMGCNRIFGIAATQPWDAGPDVTIDMPHVVLTW
                     QLATTSPSGAPSPQLEYPPFAAGVAPQIRIATLDGPFMPPLASYSSDAATPGWILVPR
                     SYFEPSGSPPAIRPWRLEYTLPGGIPHEVQWAPDDKTSHLVVPMVGRLDRVAVPAGSG
                     YTVTWTSSFTFTGMDTPRVLTTGLWTTGLTGMPLAGAKMVDYDFGNATSLSGPKGSPD
                     PAQGDRGLVVDYLPDPDNKGFQCNVAVASTPLTQLALSGTGHTAETAVWDTARPKVNS
                     DLPLLPIEGRLTNALGPLNTVVSRLDSQLAFGVVPSTSFPGLIGSSKDFKLPVPVMQT
                     LLQCPVGPNAPGPMDMASIPGTARPTQLGDFPTAVHVQLVDGRPVPPLTDPLFSGMET
                     VITASAGGGFQLSFPAAIPTGILLTTPAGGKVDLINSPEHVAVGAPTGAFLLDITPET
                     GVGLRADYYDVYLRRITGTALTVERIYTVTAPEVRIDGARLAPGADYVFEIRSYAGHV
                     MAPRGDFAPVDYPYGSAVVFTRTFKTS"
     gene            2036..>2284
                     /locus_tag="VF516_06350"
     CDS             2036..>2284
                     /locus_tag="VF516_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107331.1"
                     /translation="MSEPREHRAGPWLVALGAGLWGTESAWRIPLNDVFSPNVLVFWE
                     HVILVLCALPLILPRLGELRRVSGRTLAWLVFSGAAGSA"
BASE COUNT          315 a          728 c          901 g          340 t
ORIGIN      
        1 gatgacctcg accggctgga cctcgctgcc gtcgagccgc atgatcgagc ccgagccgaa
       61 ctgcttgtgg atggcgcgga tggcctctgc gatgctcttg ttcttcttgt cgtgaatcgt
      121 cttgtcctgg ttcgacatga aaacctccgc gcggggggat tccacgtcgt gtgccatcgc
      181 aaccatgcga gatcgcgagg gcggctgtcc gaaacgtcca gccggcggac gggtcgtccg
      241 gaatccggac gaaacccgga cagcggcgct acgaggtctt gaacgtgcgg gtgaacacga
      301 cggcgctgcc gtagggatag tcgaccggcg cgaagtcgcc gcgcggcgcc atcacgtggc
      361 cggcgtagga ccggatctcg aacacgtagt cggcgccggg ggcgagcctc gcgccatcga
      421 tccgcacctc gggcgcggtg acggtgtaga tccgctcgac ggtgagcgcg gtgccggtga
      481 tccggcgcag gtagacgtcg tagtagtcgg cgcgcaggcc gacgccggtc tcgggggtga
      541 tgtcgaggag gaacgcgccg gtcggggcgc cgacggcgac atgctcgggg ctgttgatga
      601 ggtcgacctt gccgccggcc ggcgtggtga gcaggatccc ggtcgggatc gccgccggga
      661 acgacagctg gaagccgccg ccggcggacg cggtgatcac ggtctccatc ccggagaaca
      721 ggggatcggt gagcggaggg acggggcggc cgtcgaccag ctggacgtgg accgcggtgg
      781 ggaagtcgcc gagctgcgtg ggccgggcgg tgccagggat cgacgccatg tccatcggac
      841 ccggcgcgtt gggaccgacc gggcactgca gcagggtctg catcaccggt accggcagct
      901 tgaagtcctt ggagctgccg atcaggccgg ggaagctggt gctcgggacg acgccgaacg
      961 cgagctgcga gtccaggcgg ctgaccacgg tgttgagcgg gccgagagcg ttggtgagcc
     1021 gcccctcgat cgggaggagg gggaggtcgc tgttcacctt cggccgcgcc gtgtcccaca
     1081 ccgcggtctc tgcggtgtgc ccggtcccgc tcagcgcgag ctgggtcagc ggggtcgagg
     1141 cgaccgcgac gttgcactgg aagcccttgt tgtcggggtc gggcaggtag tcgacgacga
     1201 gcccgcggtc gccctgcgcg gggtcggggc tgcccttggg gccgctgagc gacgtggcgt
     1261 tgccgaagtc gtagtcgacc atcttggcgc ccgccagcgg catgccggtg aggccggtgg
     1321 tccacagccc ggtggtgagc acgcgggggg tgtccatgcc agtgaacgtg aagctgctgg
     1381 tccacgtgac cgtgtagccg ctgccggccg ggacggccac gcggtcgagc cggccgacca
     1441 tcggcacgac gaggtggctg gtcttgtcgt cgggcgccca ctggacctcg tgtgggatgc
     1501 cgccgggcag cgtgtactcg agccgccacg gccggatcgc gggcgggctg ccggacggct
     1561 cgaagtagct gcgcgggacc aggatccagc ccggcgtggc ggcgtcgctg gagtagctcg
     1621 cgaggggtgg catgaacggc ccgtcgagcg tggcgatccg gatctgcggc gcgacgccgg
     1681 ccgcgaacgg cgggtactcg agctgcgggg agggcgcgcc ggagggcgag gtcgtcgcga
     1741 gctgccaggt cagcacgacg tgcggcatgt cgatcgtgac gtcgggaccc gcgtcccacg
     1801 gctgggtcgc cgcgatcccg aagatccggt tgcaacccat gagcaggagc cagccgacgc
     1861 cggccacgcg ccgcatgacg gaatcctggc acaccgccaa gcgtccgggc aatcgccgtc
     1921 aagagggcgc cggggcacgc gcgccggccg ggcggccgcg ccccgccgcg cggtcgaccc
     1981 gatcgctgga tgacgatcct cacatcatcg ggcaggcgat ggtatgttcg cggccttgag
     2041 cgagccccgc gaacatcgcg ccggtccctg gctggtcgcg ctcggcgccg gcctgtgggg
     2101 cacggagagc gcatggcgga tcccgctcaa cgacgtgttt tcccccaacg tgctggtgtt
     2161 ctgggagcac gtgatcctgg tcctctgcgc gctgccgctg atcctgccgc ggctcggcga
     2221 gctgcgccgg gtgagcggtc ggacgctcgc gtggctggtg ttctcggggg ccgcgggatc
     2281 ggcg
//
LOCUS       DASVOR010000305         6363 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_295045,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000305 DASVOR010000000
VERSION     DASVOR010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6363
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295045"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..810
                     /locus_tag="VF516_06355"
     CDS             97..810
                     /locus_tag="VF516_06355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107332.1"
                     /translation="MRCAIWAAAVGVLTAAGCSKKSQDGLPPASEWQSAPAAASAPSA
                     PDPADVIPHFTPPPGMIAKESNPHAGLDMGDMGDPSNPHAGLDMNGDGEPDNPHAGGG
                     PDVTRLGLAAPDPDRPIDPSHRIAGTLGIDPRAKDKVKPGTSVFLIVKRAGPDGAPSG
                     PPLAVDKLTWTGDGMAFELTEAQAMVAGTDLSGDVVVMARYDQDSDALSRQPGDVTGQ
                     IRVKIPAEHVKLMLDTVLP"
     gene            complement(822..1832)
                     /locus_tag="VF516_06360"
     CDS             complement(822..1832)
                     /locus_tag="VF516_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107333.1"
                     /translation="MLRGSRRIAVLLASALLAGPARPALADPRPASAKDQKLASELVK
                     KAIALAEAGDHAGAIQLYNDAYKLVPNVLLLPSIGAQYQELGQWDDALEYFCRYLKED
                     PAGVNVAFARSSAKHAQQELGRKNIDSRNPCAAGSRDDGDGGGKRKIDDGDGGGRRKI
                     DDGDGGGKRKIDDGDGKRKIDDGDGGGKRKIDDSDGGGKRKIDNGNGNGMTVDPPPAP
                     ARSNGNPVLMYTGLAAGIAGIAAGGISVYYGFQGKSISDEINSHQPGTPWPDDIQTKM
                     KEGEHDNRLQVGYMVASGVLVATGIVLYVVGRPDANERTGEKTAVHVAPTANGVTVFG
                     RF"
     gene            complement(1864..2676)
                     /locus_tag="VF516_06365"
     CDS             complement(1864..2676)
                     /locus_tag="VF516_06365"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEX8107334.1"
                     /translation="MSFERELARAIELARQAGAEAARMQRGELQVEMKPGDEPVTVAD
                     RRASELIVAGLAASFPGDPIISEELAPAPGALGAPRYWLVDPIDGTKDFIRGEDGYSV
                     MIGLVCDGVPTVGVVYQPVIDRLFHGTPEGAAMVVEGQTRRLAVSSVAAAAEVRLVAS
                     KSHRSQELDRVKRELGIDNELNVGSVGVKLCMIAAGLRDLYVNPSTRTKAWDTCAPEA
                     ILVPAGGRLSDLFGNRIDYTRELAHHRGLVASNGHVHDEVIAKLAPLFANLA"
     gene            complement(2673..3533)
                     /locus_tag="VF516_06370"
     CDS             complement(2673..3533)
                     /locus_tag="VF516_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HEX8107335.1"
                     /translation="MTAADKVIVSCALTGAVTTKKHCPAIPYTPIEIAEEARRAYEAG
                     AAIVHIHARNDDGSPTWSKDTFARIMAETKQRCPVLINWSTGGAGPMSERVSHLALRP
                     HIAALNMGSMNYAKWSEHKKQFAFNFVFANPFDDILGFARAMKEHGAKPEMECFDVGH
                     TASHRVLVELGLLEAPYHFSFIMGVLGGIPATARHLAFQAEQVPAGARWKVIGISREQ
                     WPLAMAALALGGDVRIGLEDNFYLPSGEMAKSNGELVAAAVELVRLSGRSAATVDEAR
                     QLLWPHGGSN"
     gene            3743..4252
                     /locus_tag="VF516_06375"
     CDS             3743..4252
                     /locus_tag="VF516_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107336.1"
                     /translation="MCEFASLERCAAYVAARAVLTAVQRAAPRWPEPLGHRAHRGALD
                     TLELTAQAAGHGHDSAGRRHCLREAITAALGVAATIDAVRAMGHGGDDLADLQRVTGR
                     TIALLGMFLHAGTALAGAPERARSEKNSEKTKSREPTSRSRRPTGSRDMTTPLQRSPR
                     SPSEPPVTS"
     gene            complement(4748..5113)
                     /locus_tag="VF516_06380"
     CDS             complement(4748..5113)
                     /locus_tag="VF516_06380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107337.1"
                     /translation="MARILVVEDDLWSRRLVFELLVLRGHDVLCAAGVGDGRAQLDSV
                     KFDAVLLDINIPGGGGEVLLREIREYNSVMPVIAFTASAMAGDRERFLAEGFTGHISK
                     PIDVKSFGETVEAFLLPPP"
     gene            complement(5113..>6363)
                     /locus_tag="VF516_06385"
     CDS             complement(5113..>6363)
                     /locus_tag="VF516_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8107338.1"
                     /translation="SLETWRELLARISQMYHETDQDRDELESSIESSSREMQRLYQEL
                     KQRAEAERAEQDAILRATLDSANEGILVTDNQHGVIAVNRRFVEMGQIAEEVVATRDR
                     RKTIAAALKKVKMADAMLARTDKLYESEATIRDEVELVDGSLMDWFSTPVRTPDGTKV
                     GRVAFVRDVTAERHAQQHLQRARDAAEAASQAKSQFLANMSHELRTPLTAVIGLSDLL
                     LMERDPLVPRQKEYLEGIASSGRHLLALVNDVLDLAKIEAGKTELKLESVPIHDAIEE
                     GLSTFSPLATTRGVILEPATIVAVPNVRADRVRLRQILYNLISNAVKFTDRGGRVQVR
                     ARRDDDRVSIAVMDTGVGIATRDLTRLYRTFEQLTLPSGDRPGGTGLGLALTKRLVEM
                     HGGTIDVESELGVGTTFTVRIPVA"
BASE COUNT          906 a         2455 c         1997 g         1005 t
ORIGIN      
        1 ccacgtctgg ctgggggagc cccacccagg tgcgcagggg ctggccaccg tcacgacgac
       61 ggcgcgctgg gtgtgaccct gctacactac cccgcgatgc gatgcgcgat ctgggccgcg
      121 gcggtcggcg tcctgaccgc cgcgggctgc agcaagaaga gccaggacgg cctgccgccg
      181 gcctccgagt ggcagtctgc gccggcggcg gcgagcgcgc cctccgcccc ggacccggcc
      241 gacgtcatcc cgcacttcac gccgccgccc ggcatgatcg ccaaggagag caacccgcac
      301 gccggcctcg acatgggcga catgggcgac ccgagcaacc cgcacgccgg cctcgacatg
      361 aacggcgacg gcgagcccga caacccgcac gccggcggcg gccccgacgt caccaggctc
      421 ggcctcgcgg cgcccgatcc ggaccgtccg atcgacccga gccaccggat cgccggcacg
      481 ctcggcatcg atcccagggc caaggacaag gtcaagcccg gcacctcggt gttcctgatc
      541 gtcaagcggg cggggcccga tggcgcgccc tcggggccgc cgctcgccgt cgacaagctg
      601 acctggacgg gcgacggcat ggcgttcgag ctcaccgagg cccaggccat ggtcgccggc
      661 accgacctca gcggcgacgt cgtcgtgatg gcccgttacg atcaggacag cgacgcgctg
      721 tccaggcagc cgggcgacgt caccggccag atccgcgtca agatccccgc cgagcacgtc
      781 aagctgatgc tcgacaccgt gctgccgtag ccgcgcgccg ctcagaaccg gccgaacacg
      841 gtgacgccgt tcgccgtcgg cgcgacgtgg accgccgtct tctcgccggt gcgctcgttc
      901 gcgtcgggcc ggccgaccac gtacagcacg atgccggtgg ccaccagcac gccgctcgcg
      961 accatgtagc cgacctggag ccggttgtcg tgctcgccct ctttcatctt ggtctggatg
     1021 tcgtccggcc agggcgtgcc cggctgatgg ctgttgatct catcgctgat gctcttgccc
     1081 tggaacccgt aatagacgct gatcccgccc gcggcgatgc cggcgatccc cgcggccagc
     1141 ccggtgtaca tcagcacggg attgccgttt gacctcgcag gcgccggtgg cggatcgacc
     1201 gtcatgccgt tgccgttgcc gttgtcgatc ttgcgcttgc cgccgccgtc gctgtcgtcg
     1261 atcttgcgct tgccgccgcc gtcgccgtcg tcgatcttgc gcttgccgtc gccgtcgtcg
     1321 atcttgcgct tgccgccgcc gtcaccgtcg tcgatcttgc gcctgccgcc gccgtcgccg
     1381 tcgtcgatct tgcgcttgcc gccgccgtcg ccgtcgtcgc ggctccccgc cgcgcaggga
     1441 ttgcggctgt cgatgttctt ccggccgagc tcctgctgcg cgtgcttggc cgacgaccgc
     1501 gcgaacgcca cgttgacgcc ggccggatcc tctttcaggt acctgcagaa gtactccagc
     1561 gcatcgtccc actgcccgag ctcctgatac tgcgcgccga tgctcggcag cagcaggacg
     1621 ttggggacca gcttgtacgc gtcgttgtag agctggatcg ccccggcatg atcgccggcc
     1681 tcggcgagcg cgatggcctt cttcaccagc tcgctggcca gcttctggtc cttcgcgctg
     1741 gcgggcctgg ggtccgccag ggcagggcga gccgggccgg cgagcagggc cgatgccagc
     1801 agcaccgcga tgcgtcgcga accacgcagc atgatgtagc ctatcatgcg tgatgtacgc
     1861 cggtcatgcc agattcgcga acagcggtgc cagcttcgca atcacctcgt catggacgtg
     1921 accgttcgac gcgaccaggc cgcgatggtg tgcaagctcg cgggtatagt cgatccggtt
     1981 gccaaataga tcactgagcc gtccgcccgc cgggaccagg atcgcttccg gcgcgcaggt
     2041 gtcccaggcc ttggtccgcg tcgacgggtt gacgtagagg tcgcgcagcc ccgcggcgat
     2101 catgcacagc ttcacgccga ccgagccgac gttgagctcg ttgtcgatgc cgagctcgcg
     2161 cttgactcga tcgagctcct gcgagcggtg ggacttcgag gcgaccaggc ggacctcggc
     2221 cgccgccgcg acgctcgaca ccgccagccg ccgggtctgt ccctcgacga ccatcgccgc
     2281 gccctccggc gtgccgtgga acagccgatc gatcaccggc tggtacacca cgccgaccgt
     2341 cggcacgccg tcgcacacca ggccgatcat caccgagtaa ccgtcctcgc cgcggatgaa
     2401 gtccttggtg ccgtcgatcg gatcgaccag ccagtagcgc ggcgcaccga gcgcgcccgg
     2461 cgccggggcg agctcctccg agatgatcgg atcgcccgga aagctcgccg cgagcccggc
     2521 gacgatcagc tccgacgccc ggcggtcggc caccgtcacc ggctcgtccc cgggcttcat
     2581 ctcgacctgc agctcgccgc gctgcatccg cgcggcttcc gcgccggcct gccgcgccag
     2641 ctcgatcgcc cgcgccagct cgcgctcgaa gctcaattgc tgcccccgtg cggccacagc
     2701 agctgccggg cctcgtcgac cgtggccgcg ctgcgccccg acagccggac cagctcgacc
     2761 gcggccgcga ccagctcgcc gttggacttc gccatctcgc cgctgggcag gtagaagttg
     2821 tcctcgaggc cgatccggac gtcgccgccg agcgccagcg ccgccatcgc gagcggccac
     2881 tgctcgcggc tgatcccgat caccttccag cgcgcccccg ccggcacctg ctcggcctgg
     2941 aacgccaggt ggcgcgcggt cgccgggatc ccgccgagca cgcccatgat gaacgagaag
     3001 tggtacggcg cttccagcag cccgagctcg accagcaccc ggtgcgaggc cgtgtgcccg
     3061 acgtcgaagc actccatctc gggcttggcc ccgtgctcct tcatcgcgcg ggcaaacccg
     3121 aggatgtcgt cgaacgggtt cgcgaacacg aagttgaacg cgaactgctt cttgtgctcg
     3181 ctccacttgg cgtagttcat cgagcccatg ttcagcgccg cgatgtgcgg ccgcagcgcg
     3241 aggtggctca cccgctcgga catcggtccg gcgccgcccg tcgaccagtt gatcagcacc
     3301 gggcagcgct gcttggtctc ggccatgatc cgcgcgaacg tgtccttcga ccacgtcggc
     3361 gagccgtcgt cgttgcgggc gtggatgtgc acgatcgccg cgccggcctc gtacgcccgc
     3421 cgcgcctcct cggcgatctc gatcggcgtg tacgggatcg cggggcagtg cttcttggtg
     3481 gtcacggcgc cggtcagggc gcacgacacg atgactttgt cggcggcggt cacgccgtga
     3541 cagttgcggc aagccgggtg aacggtcaag tccctcctgc gagtcggtcc ggccgcgcca
     3601 tcgcccgaga gctccggcgc cacgcacgcc atgccggcgt ccggcgttgc cacccagctg
     3661 tccgcccagc ggacgttccg gacagcgccg cacgcgatct cagctagtta cgcaggcgcg
     3721 gggcatgctg gggcgcccgt ccatgtgcga gttcgcgagc ctggagcgct gcgccgccta
     3781 cgtggccgcg cgcgctgtgc tgaccgccgt tcagcgcgcc gctccgcggt ggccggagcc
     3841 cctgggccac cgtgcccacc gcggcgcgct cgacaccctg gagctcaccg cgcaggccgc
     3901 cggtcacggt catgactcgg ccggccgccg gcactgcctg cgcgaggcga tcaccgccgc
     3961 gctgggcgtc gccgcgacca tcgacgccgt gcgcgcgatg ggccatggcg gcgacgacct
     4021 tgccgatctg cagcgcgtca ccggccgcac catcgcgctg ctcggcatgt tcctccacgc
     4081 cggcaccgcg ctcgccggag cgccggagcg cgctcgatcc gaaaaaaact ccgaaaaaac
     4141 caagtcgcgt gagccgactt cccgctcccg acgcccgacc ggctcacgcg acatgaccac
     4201 cccattgcaa cgcagtcccc gatcccccag cgaaccccct gtaacctcgt gatccaccag
     4261 cctccgaccc cgctgctccc gccacccgca ctcgccccgg ccacctcgcc gccgctcccc
     4321 cgacccgccc agcctccgcc tgcctccgcc tgcctccgcc cgacccactc cctcccgcgt
     4381 ccacgcgtct ctatgccccc cgcaactccg cgattcccaa ccgacctcca gcaccaccgt
     4441 cgccaccgct ctctcagccc ctacctcgca ggacgggctt ctcccagtgc gtgatacgtg
     4501 ccaaggctta caggccgaag ttgcgctggt tccgtagcgc acagtgtcca gcccaatgga
     4561 caaccgacct gtaggaccgt ccactgcagt ggacactctg tttcagcgtc cgggcgccgc
     4621 gcgatcgggg catgcctcgg agcgaatcga cctcaagatc acctcaaccg tattccagcc
     4681 aacgatccgt gggtgacgga tttgtcccgc gcgtccgtcc ccctgtcaac tgtaaaacgg
     4741 gtgtgtttca tggcggtggt aacaagaacg cctccaccgt ctcgccgaac gacttcacgt
     4801 cgatcggctt gctgatgtgc ccggtgaagc cctcggcgag gaaccgctcg cggtcgcctg
     4861 ccatcgccga cgccgtgaac gcgatcaccg gcatcaccga gttgtactca cggatctcgc
     4921 gcagcaagac ctcgccaccg ccgccgggga tgttgatgtc gagcagcacg gcatcgaact
     4981 tcaccgagtc gagctgggcc cggccatcgc cgaccccggc tgcgcacagc acatcgtgcc
     5041 cgcgcagcac cagcagctcg aacacgagcc ggcggctcca cagatcgtct tcgacaacga
     5101 gaatgcgcgc catcaggcca ccggaatcct cacggtgaag gtcgtaccga cgccgagctc
     5161 gctctcgacg tcgatggttc caccgtgcat ctccacgagt cgtttggtca gtgcgagccc
     5221 cagtcccgtc ccacccggac gatcgcccga cggcagcgtc agctgctcga acgtgcggta
     5281 cagccgcgtc agatctcgcg tcgcgatccc gactccggta tccatcaccg cgatcgacac
     5341 ccggtcgtcg tcgcgccggg cgcggacctg cacccggccg ccgcgatcgg tgaacttcac
     5401 cgcgttcgag atcaggttgt agaggatctg gcgcagccgc acgcggtcgg cgcgcacgtt
     5461 gggcaccgcc acgatcgtcg ccggctcgag gatcaccccg cgcgtcgtcg ccagcggcga
     5521 gaacgtcgac aggccttcct cgatcgcgtc gtggatcggg accgactcca gcttcagctc
     5581 ggtcttgccg gcctcgatct tggcgaggtc gagcacgtcg ttgaccagcg cgagcaggtg
     5641 ccggccgctc gacgcgatcc cctccaggta ctccttctgc cgcggcacca gcggatcgcg
     5701 ctccatcagc aacaggtccg acagcccgat caccgcggtc agcggcgtcc gcagctcgtg
     5761 cgacatgttc gcgaggaact ggctcttggc ctggctggcc gcctccgccg catcgcgcgc
     5821 tcgctgcagg tgctgctggg cgtgccgctc ggcggtcacg tcacggacga acgccacccg
     5881 gcccaccttc gtgccgtccg gcgtccggac cggcgtgctg aaccagtcca tcagcgagcc
     5941 gtccaccagc tcgacctcgt cgcggatcgt cgcctcgctc tcgtacagct tgtcggttcg
     6001 cgccagcatg gcgtcggcca tcttcacctt cttcagcgcc gccgcgatcg tcttgcgccg
     6061 gtcgcgcgtc gccaccacct cctcggcgat ctgccccatc tccacgaacc gccggttcac
     6121 cgcgatcacg ccgtgctggt tgtcggtcac caagatcccc tcgttcgccg agtccagcgt
     6181 cgcgcgcagg atcgcgtcct gctcggcgcg ctcggcctcc gcccgctgct tcagctcctg
     6241 atacagccgc tgcatctcgc gcgacgaact ctcgatcgag ctctccagct catcgcgatc
     6301 ctggtcggtc tcgtgataca tctggctgat ccgggccagc agctcgcgcc aggtctccag
     6361 cga
//
LOCUS       DASVOR010000306         3056 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_178214,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000306 DASVOR010000000
VERSION     DASVOR010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178214"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /gene="hybB"
                     /locus_tag="VF516_06390"
     CDS             <1..991
                     /gene="hybB"
                     /locus_tag="VF516_06390"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HEX8107339.1"
                     /translation="YDDVIGTALGASGFVVAFATYVLNRGAYHPLVRPALVTALFGYV
                     MASASVFVDIGRYWQAWHIFVPGYAQFNSVLLLVALCIIACTGILFVELLPAVLERFG
                     MHAIRRRLDRVMFLFIALGLVLAAVQQSSFGSLLIVFGPQIDPVYQTRLLPVLFLTST
                     IGMGLAAVVIEGSVSALALRRPLERELLARLMKVGQASVQIFLVARFADLAIRGALPR
                     VVEPTAVAVMFWIETVLFVAPIAFLGGPRGNRPARLFLAAMAMAIAGMLYRIDAYLVA
                     YHTGAGWHYFPSLGELAVTVGLIAAEILAFILIIRLFPVLPRLHRSNPGSGPA"
     gene            1006..2709
                     /locus_tag="VF516_06395"
     CDS             1006..2709
                     /locus_tag="VF516_06395"
                     /inference="COORDINATES: protein motif:HMM:NF012592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HEX8107340.1"
                     /translation="MAKRIVIDPITRIEGHLRIDCEVNAGKVTNAWASGTMWRGVEQI
                     LLGRDPRDAWAITQRFCGVCTTVHAIASVRAVENALQMAVPRNAQYIRNLMIAAHAIH
                     DEIVHFYHLSALDWVDITSAAKADPRATADLAESLSGWPMNGAKVHAATKERLGSIVA
                     SGQLGIFANGYWGHPAMKLPPEVNLLAVTHYLQALEVQRKANKIVSILGSKTPHIQNV
                     AVGGVSNPIALDSPSVLSLERLLAVKQAIDELKQFITEVYTIDVAAVAAFYPEWTKIG
                     RGVVNYLAAPDFPLDEKGTAFVTPGGYIPAGDLAAMKPITTFGDALLRDNVAESVRHA
                     WYQGGKGPLHPYRGETVAEYTGFETTGKYSFVKSPTFAGKPAQVGPLARVLVAAAARD
                     EAILGHLNRVIGTVEAIAKIKLPLDALHSTLGRHAARAVMCARNVEILEHQWDLLMAN
                     IAKGDTATFEKPVFPDHEVSGFGFHEAPRGLLSHWVVIDKGVIKNYQAVVPTTWNAAP
                     RNEDDQLGPYEASLLGTPLADPEQPLEVLRTIHSFDPCLACAVHLVDPARRDIIQVRA
                     L"
     gene            2710..>3056
                     /locus_tag="VF516_06400"
     CDS             2710..>3056
                     /locus_tag="VF516_06400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HEX8107341.1"
                     /translation="MAARAVTGRAVVLGVGNVIMADEGVGVRCVERLEVTGALPAGAT
                     AIDGGTSTHELLGELEDLDLLVIVDAVASGGAPGSLVRLEGDRIPSAFSNRLSPHQHG
                     INDLLATLRLIDRA"
BASE COUNT          440 a         1088 c         1039 g          489 t
ORIGIN      
        1 ctacgacgac gtcatcggca ctgccctggg cgccagcggc ttcgtcgtcg cgttcgcgac
       61 ctacgtcctg aaccgcggcg cctaccatcc gctggtccgg cccgcgctgg tgaccgcgct
      121 gttcggctac gtcatggcga gcgcgtcggt gttcgtcgac atcggccgct actggcaagc
      181 gtggcacatc ttcgtgccgg gctacgcgca gttcaactcg gtcctgttgc tggtggcgct
      241 gtgcatcatc gcctgcaccg ggatcctgtt cgtcgagctc ctgccggccg tcctcgagcg
      301 gttcggcatg cacgcgatcc gccgccggct cgaccgcgtg atgttcctgt tcatcgcgct
      361 cggactggtc ctggcggcgg tacagcagtc gtcgttcggc tcgctgctga tcgtgttcgg
      421 tccgcagatc gatccggtct accagacgcg gttgctgccg gtgctgttcc tgacctcgac
      481 gatcggcatg ggcctcgccg ccgtggtgat cgagggcagc gtgtccgcgc tggcgctgcg
      541 acgcccactg gagcgcgagc tgctcgccag gctcatgaag gtcggccagg cgtccgtgca
      601 gatcttcctg gtcgcgcggt tcgccgatct cgcgatccgc ggcgcgctcc cccgggtggt
      661 cgagccgacc gcggtcgcgg tcatgttctg gatcgagacc gtgctgttcg tggcaccgat
      721 cgcgttcctc ggcggtccgc ggggcaaccg cccggcgcgg ctgttcctcg ccgcgatggc
      781 gatggcgatc gccggcatgc tgtaccgcat cgacgcctac ctcgtcgcgt atcacaccgg
      841 cgccggctgg cactacttcc cgtcgctggg cgagctggcg gtcacggtcg gcctgatcgc
      901 cgccgagatc ctcgccttca tcctcatcat ccggctgttc ccggtgctgc cgcggctgca
      961 ccgctcgaac cccggctcgg gtccggcctg agcgaaggac gcaccatggc aaagcgcatc
     1021 gtcatcgacc ccatcacccg catcgagggt cacctccgga tcgattgcga ggtcaacgcc
     1081 ggcaaggtga ccaacgcctg ggcgtcgggc acgatgtggc gcggcgtcga gcagatcctg
     1141 ctcggccgcg atccgcgcga cgcgtgggcg atcacccagc gattctgcgg cgtgtgcacc
     1201 accgtgcacg cgatcgcgtc ggtccgcgcg gtcgagaacg cgctgcagat ggcggtgccc
     1261 aggaacgccc agtacatccg caacctgatg atcgcggcgc acgcgatcca cgacgagatc
     1321 gtgcacttct accacctgtc ggcgctcgac tgggtcgaca tcacctcggc cgccaaggcc
     1381 gatcccaggg cgaccgccga tctcgccgag agcctgtcgg gctggccgat gaacggcgcc
     1441 aaggtgcacg ccgcgaccaa ggagcggctg ggcagcatcg tcgcgagcgg ccagctcggg
     1501 atcttcgcca acggctactg gggccacccc gcgatgaagc tgccgccgga ggtcaacctg
     1561 ctcgccgtca cccactacct gcaggcgctc gaggtccagc gcaaggccaa caagatcgtc
     1621 agcatcctcg gcagcaagac cccgcacatc cagaacgtcg cggtcggcgg cgtgtccaac
     1681 ccgatcgcgc tcgacagccc gtcggtgctc tcgctggagc ggctgctcgc ggtcaagcag
     1741 gcgatcgacg agctcaagca gttcatcacc gaggtctaca cgatcgacgt cgccgcggtc
     1801 gcggcgttct acccggagtg gaccaagatc ggcaggggcg tcgtgaatta cctggccgcg
     1861 cccgacttcc cgctcgacga gaagggcacg gcgttcgtga ccccgggcgg gtacatcccg
     1921 gccggcgacc tcgcggcgat gaagccgatc accacgttcg gcgacgcgct gctgcgcgac
     1981 aacgtcgccg agagcgtccg gcatgcctgg tatcagggcg gcaaggggcc gctccatccg
     2041 taccgcggcg agaccgtggc cgagtacacc gggttcgaga ccaccgggaa gtactcgttc
     2101 gtcaaatctc cgacgttcgc cggcaagccc gcgcaggtcg gcccgctcgc ccgcgtgctg
     2161 gtcgcggccg cggccaggga cgaggcgatc ctcggccacc tgaaccgcgt gatcggcacc
     2221 gtcgaggcga tcgcgaagat caagctgccg ctcgacgcgc tgcactccac gctcggccgc
     2281 cacgccgcgc gcgccgtgat gtgcgcccgc aacgtcgaga tcctcgagca ccagtgggat
     2341 ctgctgatgg ccaacatcgc caagggcgac accgccacct tcgagaagcc ggtattcccc
     2401 gatcacgagg tctccggttt cgggttccac gaggcgccgc gcgggctgtt gtcgcactgg
     2461 gtggtcatcg acaagggagt gatcaagaat taccaggccg tggtcccgac cacctggaac
     2521 gccgcgccgc gcaacgagga cgatcagctc ggtccgtacg aggcgtcgct gctgggcacg
     2581 ccgctcgccg accccgagca gccgctcgag gtgctgcgca cgatccactc gttcgatccg
     2641 tgcctggcgt gcgcggtcca cctggtcgat ccggcgcgcc gcgacatcat ccaggtcagg
     2701 gccctgtaga tggcggcgcg cgctgtcacc ggccgcgccg tggtgctggg cgtcggcaac
     2761 gtgatcatgg ccgacgaggg ggtgggggtg cgctgcgtcg agcggctgga ggtgaccggc
     2821 gcgctgccgg cgggggcgac ggcgatcgac ggcgggacct cgacgcacga gctgctcggc
     2881 gagctcgagg acctcgacct gctggtgatc gtcgacgcgg tcgcatccgg cggcgcgccg
     2941 ggcagcctgg tgcgcctcga gggcgaccgg atcccgtcgg cgttctcgaa ccggctgtcg
     3001 ccgcaccagc acggcatcaa cgacctgctc gcgacgctgc ggctgatcga ccgggc
//
LOCUS       DASVOR010000307         4547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382632,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000307 DASVOR010000000
VERSION     DASVOR010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382632"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VF516_06405"
     CDS             <1..499
                     /locus_tag="VF516_06405"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEX8107342.1"
                     /translation="HHAGLVLLYPYLPRFFESTQVKPAGAPALLASELPRAAALLHLV
                     ATGDPEPFELEIDVIKLLIGLPLEAALPVSDGLVRPADREEVDGLLGAVVEHWSALKR
                     TSVQGLRVSFLQRHGLLREDEHGFRLQVEPAPFDMLLGQLPWGISVVKLPWMTRPLFT
                     DWPAP"
     gene            496..1833
                     /locus_tag="VF516_06410"
     CDS             496..1833
                     /locus_tag="VF516_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8107343.1"
                     /translation="MKDNAHDLERDLAWFAEVLEARLGAYFATEGPRRDARQIAPPSL
                     DGRQSDYARFLRTHDVPPSIRLILLLALIPHVRPQMLDVLWTRNEATQRGFTEFGGVH
                     GTSHGGFIPTGETAMFLLAGDDLAARFEAMRLFEGDHFLARHGVVHLAAAGHGEPQLS
                     GVLTISREHLHRFTTGLDRKPTYSSEFPARLISTERAWKDLVLPGSTLAQLEEIKHWV
                     LHGQSLLHDWEMTDKLRPGFTTLFHGPSGTGKTLSACLLGKHCGCDVYKIDLSMIVSK
                     YIGETEKNLARVFDMAEHKRWMLFFDEADALFGKRTRIEDARDRFANQEISFLLQRIE
                     DFDGVVILASNLKSNIDDAFLRRFHSIVHFPVPKQAERRRIWSEAFPRKAQLESGIDL
                     GRIAERYDVTGGMIMNVVRYASLMALSRGGNTVVLDDIEEGIRRELLKEGRMV"
     gene            1834..>4547
                     /locus_tag="VF516_06415"
     CDS             1834..>4547
                     /locus_tag="VF516_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107344.1"
                     /translation="MGDTAPHKTVARPAVARPAPAAVAAWAAPARTASVAGPAPLAPR
                     LESPYIARFAGSIRQMQGRGAPAAEPGDGDRFFRTRDGQVVELPDGVTAADAAKLEAE
                     ARAAEKKLGQGPPPRPVPDVKKQLGKPARKEAVKPPAPGKRAARPGKAPSSAAAAATA
                     AAGLLRAVGTSKVAQYLAQRGAPALCTGISLLHQLRRNEQTHDSAADKRQQSENAVVI
                     PPSEGHSKSNSAQVTAVDARPAPVADEKKAKATLVSTLADSVPRSIEDVDNFKRDQKA
                     EHVGAGVLKVVQGDSNAVTSTFQDVTEAPPPAPPEHSPEDLPPEELAPATAQMNLGQG
                     AVAPLQPEHTDVSNFTKDADGKLAAEGVTQQQLDMVDSGELAEANKEKKGLETRAKTE
                     PAAVQKFAAQQAQAVDQQLRQDEHAGRDAMRATRKAALRNTAQKQKGTKTALEKKREE
                     VAAKINGIYEAAQAKVKKKLADLEAESMKRFDAGNAAASKAFEDNVHRELDAYKDDRY
                     SGWFGWARKAKDWLLGMDELPGVKAIFDRNRQIFVDTVNALVEGITADQKRVIQECKD
                     ELAAARKAIKEYVDSLGPGLKDIGQKAAEDMTAKLAALDELVARKDEELQAKLKDKQA
                     AAIKAIDQKIEKMKEEMSGALAKLGKLLLLAAKKFFTWALEKVGFSLSTIEDIINKGA
                     AVLKAIFTQPIQFVKNLVRAASTGFENFGKNFLTHLKNAVFEWLTGSLEGLVLPSTWD
                     FKGILGVVFQMLGITYANFRAHLVRLIPEPVVKALETTFTLVKTLVTEGPMAAWEQLK
                     EIASEMKDAFIEAVKDWIKWKIVQKAIETVLAMFIPGAGIIRAIIGIYDTIVFFIQRA
                     KDILQMIGNFLGSIAEIAAGNIGAAAAALEAGLARGLVLVIDFLARFLR"
BASE COUNT          800 a         1591 c         1555 g          601 t
ORIGIN      
        1 ccatcacgcg ggcctcgtgc tcctgtaccc ctacctcccg cggttcttcg agagcacgca
       61 ggtcaagcca gccggcgcgc ccgcgctgct cgccagcgag ctgccgcgcg ccgcggcgct
      121 gctgcacctc gtcgccaccg gcgatccgga gccgttcgag ctcgagatcg acgtcatcaa
      181 gctgctgatc ggcctgccgc tcgaggcagc gctgccggtc tccgacgggc tcgtccgtcc
      241 cgccgaccgc gaggaggtcg acggcttgct cggcgcggtc gtcgagcact ggagcgcgct
      301 caagcgcacc tcggtgcagg gccttcgcgt ctcgttcttg caacgccacg ggctgctgcg
      361 cgaggacgag cacggcttcc ggctccaggt cgagccggcg ccgttcgaca tgctgctcgg
      421 ccagctgccc tgggggatct ccgtcgtcaa gctgccctgg atgaccagac ccctcttcac
      481 ggactggccg gcgccatgaa ggacaacgcg cacgacctgg agcgcgatct cgcctggttc
      541 gccgaggtcc tcgaggcgcg cctcggtgcg tacttcgcca ccgaggggcc ccggcgcgat
      601 gcccggcaga tcgcgccgcc gtcgctcgac ggcaggcagt ccgactatgc gcgcttcctg
      661 cgcacccacg acgtcccgcc gtcgatccgg ctgatcctcc tgctcgcgct gatcccgcac
      721 gtgcggccgc agatgctcga cgtgctgtgg acgcgcaacg aggccaccca gcgcggcttc
      781 accgagttcg gcggcgttca cggcacgagt cacggcggct tcatccccac gggcgagacc
      841 gcgatgttcc tgctcgcggg cgacgacctc gcggcgcggt tcgaggcgat gcggctgttc
      901 gagggcgatc acttcctcgc gcgccacggc gtggtccacc tcgcggcggc gggccacggc
      961 gagcctcagc tcagcggcgt gctgacgatc tcgcgcgagc acctgcaccg cttcacgacc
     1021 gggctcgacc gcaagccgac gtacagcagc gagttcccgg cgcgcctgat ctcgaccgag
     1081 cgcgcgtgga aggacctggt gctgcccgga tccacgctgg cccagctcga ggagatcaag
     1141 cactgggtgc tgcacggcca gtcgctgctg catgactggg agatgaccga caagctgcgc
     1201 ccggggttca ccacgctgtt ccacggcccg tccgggaccg gcaagacgct gtcggcgtgc
     1261 ctgctcggca agcactgcgg ctgcgacgtc tacaagatcg atctcagcat gatcgtgtcc
     1321 aagtacatcg gcgagaccga gaagaacctg gcgcgcgtgt tcgacatggc cgagcacaag
     1381 cgctggatgc tgttcttcga cgaggccgac gcgctgttcg gcaagcgcac gcggatcgag
     1441 gacgcgcgcg atcggttcgc gaaccaggag atcagcttct tgctccagcg catcgaggac
     1501 ttcgacggcg tggtgatcct ggcctccaac ctcaagagca acatcgacga cgcgttcctc
     1561 cgcaggttcc actcgatcgt ccacttcccg gtgcccaagc aggccgagcg ccggcggatc
     1621 tggagcgagg cgttcccgcg caaggcgcag ctggagtcgg gcatcgacct cggccggatc
     1681 gccgagcgct acgacgtcac cggcgggatg atcatgaacg tcgtgcgata cgcctcgctg
     1741 atggcgctga gccgcggcgg caacaccgtc gtgctcgacg acatcgagga gggaatccgc
     1801 cgcgagctgc tcaaggaggg gcgcatggtc tagatggggg acaccgcgcc gcacaagacg
     1861 gtggctcgcc ccgcggtggc acgacctgcg ccggccgccg tcgcggcgtg ggctgcaccc
     1921 gcgcgcacgg cgtccgtcgc cggtccagcg ccgctggcac cccggctcga atcgccgtac
     1981 atcgcgcggt ttgccggctc gatcaggcag atgcagggcc gcggcgcgcc cgcggccgag
     2041 cccggcgacg gcgatcgctt cttccgcacc cgcgacggcc aggtggtcga gctgccggac
     2101 ggcgtgaccg ccgccgacgc cgccaagctc gaggccgagg ccagggccgc cgagaagaag
     2161 ctcggccagg gcccgccgcc ccggcccgtc cccgacgtca agaagcagct cggcaagccg
     2221 gccaggaagg aggccgtcaa gccgccggcg ccgggcaaga gagccgccag gcccggcaag
     2281 gcaccgtcga gcgcggcagc ggcggccacc gcggcggccg ggctgctccg ggcggtgggc
     2341 accagcaagg tggcgcagta cctcgcccag cggggcgcac ccgcgctgtg caccggcatc
     2401 agcctgctgc accagctgag gcgcaacgag cagacccacg acagcgccgc ggacaagcgg
     2461 cagcagtccg agaacgcggt ggtcatcccg cccagcgagg gccactcgaa gagcaactcg
     2521 gcgcaggtga ccgcggtcga cgcgcgaccc gcgccggtcg ccgacgagaa gaaggccaag
     2581 gccacgctgg tgtccacgct ggccgacagc gtcccgcgca gcatcgagga cgtcgacaac
     2641 ttcaagcgcg atcagaaggc cgagcacgtc ggcgccggcg tgctcaaggt cgtgcagggg
     2701 gacagcaacg cggtgacctc cacgttccag gacgtgaccg aggcgccgcc gccggcgcct
     2761 cccgagcaca gccccgaaga tctgcccccc gaagagctcg cgccggcgac cgcccagatg
     2821 aacctcggcc agggcgccgt ggcgccgctc cagccggagc acaccgacgt cagcaacttc
     2881 acgaaggacg ccgacggcaa gctcgcggcg gagggcgtca cccagcagca gctcgacatg
     2941 gtcgacagcg gcgagcttgc cgaggccaac aaggagaaga agggcctcga gaccagggcc
     3001 aaaaccgagc cggccgccgt gcagaagttc gccgcgcagc aggcccaggc ggtcgaccag
     3061 cagctccgcc aggacgagca cgccggccgc gacgcgatgc gcgccacgcg caaggcggcg
     3121 ctgcgcaaca ccgcgcagaa gcagaagggc accaagaccg cgctcgagaa gaagcgcgag
     3181 gaggtcgccg ccaagatcaa cggcatctac gaggccgcgc aggccaaggt caagaagaag
     3241 ctcgccgatc tcgaggccga atcgatgaag cgattcgacg ccggcaacgc ggctgcttcg
     3301 aaggcgttcg aggacaacgt ccaccgcgag ctcgatgcct acaaggacga tcgctactcc
     3361 ggctggttcg gctgggcgcg aaaggccaag gactggctgc tcggcatgga cgagctgccc
     3421 ggggtcaagg cgatcttcga tcgcaaccgc cagatcttcg tcgacacggt caacgccctc
     3481 gtcgagggca tcaccgccga ccagaagcgg gtgatccagg agtgcaagga cgagctcgcc
     3541 gccgcgcgca aggcgatcaa ggaatacgtc gattcgctcg gccccggcct caaggacatc
     3601 ggccagaagg ccgccgagga catgaccgcg aagctcgccg cgctcgacga gctcgtcgcc
     3661 cgcaaggacg aggagctcca ggccaagctc aaggacaagc aggccgcggc gatcaaggcg
     3721 atcgatcaga agatcgagaa gatgaaggag gagatgtcgg gcgcgctggc caagctcggc
     3781 aagctgctgc tgctggccgc caagaagttc ttcacctggg cgctcgagaa ggtcgggttc
     3841 tcgctgtcga cgatcgagga catcatcaac aagggcgcgg cggtgctcaa ggcgatcttc
     3901 acgcagccga tccagttcgt gaagaacctg gtgcgcgccg cgagcaccgg gttcgagaac
     3961 ttcggcaaga acttcctgac ccacctcaag aacgccgtgt tcgagtggct gaccggctcg
     4021 ctcgagggcc tggtcctgcc gtcgacctgg gacttcaagg ggatcctcgg cgtcgtgttc
     4081 cagatgctcg gaatcaccta cgcgaacttc cgcgctcacc tggtccggct gatccccgag
     4141 ccggtggtga aggcgctcga gacgaccttc acgctggtca agaccctcgt caccgagggc
     4201 ccgatggcgg cgtgggagca gctcaaggag atcgccagcg agatgaagga cgccttcatc
     4261 gaggcggtga aggactggat caagtggaag atcgtgcaga aggcgatcga gaccgtgctc
     4321 gcgatgttca tcccgggcgc cggcatcatc cgtgcaatca tcggcatcta cgacacgatc
     4381 gtgttcttca tccagcgggc gaaggacatc ctgcagatga tcggcaactt cctgggctcg
     4441 atcgccgaga tcgcggccgg caacatcggc gcggcggcgg cggcgctcga ggccggcctg
     4501 gcccgcggcc tggtgctggt gatcgacttc ctcgcccggt tcctgcg
//
LOCUS       DASVOR010000308         3736 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_236489,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000308 DASVOR010000000
VERSION     DASVOR010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236489"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1132
                     /locus_tag="VF516_06420"
     CDS             200..1132
                     /locus_tag="VF516_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107345.1"
                     /translation="MGKLVVAHAERRAQRTLQRLAGATLSPVEVVADGHALMAAVDPD
                     TIAIVDLALVRAHPGLHDRPARAWIAVPGEGAEPAEAEIVERLLATGWWHIISHPMPV
                     LAEELLATVQKLRRREVFGLEKYVAWGAETRSYTLGDADERDDAVGMVTRDVLGVGLP
                     DRIGSLVSVITDELIANALYLAPLDERKRRYRAGEERARSRPLRGRDVVTVRWATDAR
                     YLAIEVRDRWGTLEPRSVASRLAAGRVKTTGAPGDTGMGLALAYACCNQFVIDLEPEV
                     MTEVIALLDVRHRPIELGRCASFHTFLAEPPHES"
     gene            1182..>3736
                     /gene="gcvP"
                     /locus_tag="VF516_06425"
     CDS             1182..>3736
                     /gene="gcvP"
                     /locus_tag="VF516_06425"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEX8107346.1"
                     /translation="MNPDILAPSDTFARRHISPSQAEIDQMLAALGYHSLDELATAVV
                     PGAIRLARPLALGERLPLGEHELLEEMKNLAADNVVLRSFIGQGYHDTITPPVILRNV
                     LENPGWYTQYTPYQPEISQGRLEALINFQTLVEDLTALPMANASLLDEATAAAEAMTM
                     CVAHAAERGDRDARAARHTFWVQAETHPQTIAVMHTRAEPLGVALRVGTAAEIAAGLG
                     PEVAGVLLSYPTTDGRIDDHRALIAQLHAAGAAVVMATDLLALTLLVPPGELGADVAI
                     GSAQRFGVPMGFGGPHAAFLAARDDFRRQMPGRIIGVSRDAKGKTAYRLSLQTREQHI
                     RREKATSNICTAQVLLAVMASMYAVYHGPDGLVRIAERVRGFTAVIAAGLARLGYRVR
                     AGAYFDTLRIDLDAQRRAEVLARALDRGLNLRRYDDGVGISCDETTSQADVHDLLEAF
                     ARGDELPFEVAALAAETELPPLALRRSSAFLTHPTFHRYHSEHEMLRYLNRLTTKDLS
                     LTTSMIPLGSCTMKLNATAEMLPVTWAEFGRLHPFAPPEQWGGYRAMFEQLEAWLAEI
                     TGFAAVSLQPNSGAQGEYAGLLAIRSYHRARGEGHRTVCLIPQSAHGTNPASAVLAGM
                     QVVVVASAADGTIDMADLRAKAEKHAGQLGALMVTYPSTHGVFEGTIREVCEVVHRHG
                     GQVYMDGANMNAQVGLTRPADIGADVCHLNLHKTFCIPHGGGGPGMGPIAVARHLAAY
                     LPGHPLAEPGSQPTGPVSAAPWGSASILPISFAYIAMMGEPGLRRATQVAILGANYIG
                     HRLNPHFPVLYTGQNGRVAHELILDCRGFKKTAGIEAEDIAKRLMDYGFHAPT"
BASE COUNT          508 a         1389 c         1337 g          502 t
ORIGIN      
        1 ggtgctggtg atcgcgcacc cggccgcgac ccggctgcgc gcgatcggcg tggcgatcgc
       61 cgtcgccgcc gcaggctcgg gcgcggtcgc cctgctggtg gttgcgcggt agccgtcggg
      121 ccgacatcgt tgtcatcgga cgctgcatcc gcgtgccgcg tccggccacg gcgtccggcg
      181 gtgctgatac attcgggtcg tgggcaagct ggtggtcgca cacgccgagc gcagagcgca
      241 gcgcaccctc cagcggctgg cgggcgccac gctgtcgccg gtcgaggtcg tcgccgacgg
      301 ccacgcgctg atggcggcgg tcgaccccga cacgatcgcg atcgtcgacc tcgccctcgt
      361 gcgggcccac ccggggctgc acgaccgtcc agcgcgggcg tggatcgcgg tgccgggcga
      421 gggcgccgag cccgcggagg cggagatcgt cgagcggctg ctcgcgaccg gctggtggca
      481 catcatctca catccgatgc cggtcctcgc cgaggagctg ctcgccacgg tgcagaagct
      541 gcgccggcgc gaggtgttcg ggctcgagaa gtacgtcgcg tggggcgccg agacccggag
      601 ctacacgctc ggcgacgcgg acgagcgcga tgacgcggtc ggcatggtga cccgcgacgt
      661 gctcggggtc gggttgcccg atcgcatcgg atcgctggtc agcgtgatca ccgacgagct
      721 gatcgccaac gcgctgtacc tcgccccgct cgacgagcgc aagcggcgct atcgcgcggg
      781 cgaggagcgc gcgcgcagcc gccccctgcg cggccgcgac gtggtcaccg tgcggtgggc
      841 gaccgacgcg cgctacctgg cgatcgaggt gcgcgatcgc tggggcacgc tggagccgcg
      901 cagcgtggcg agccggctgg cggccggccg ggtcaagacg accggtgcgc cgggggacac
      961 cggcatgggc ctcgcgctgg cctacgcgtg ctgcaaccag ttcgtgatcg acctggagcc
     1021 cgaggtgatg accgaggtga tcgccttgct cgacgtccgc caccggccga tcgagctcgg
     1081 ccgctgtgcg tcgttccaca ccttcctcgc cgagccgccg cacgagtcgt gacaacggtc
     1141 cgggtatttt catcgccggg ccggcgcggt agggtgcgcc ggtgaatcct gacatcctgg
     1201 ctccgtccga cacgttcgcg cgccgccaca tctcgcccag ccaggccgag atcgaccaga
     1261 tgctcgccgc gctcggctac cacagcctcg acgagctggc gaccgcggtg gtccccggcg
     1321 cgatccggct cgcccggccg ctcgcgctcg gcgagcgcct gccgctgggc gagcacgagc
     1381 tcctcgagga gatgaagaac ctcgcggccg acaacgtcgt gctgcgctcg ttcatcggcc
     1441 agggctacca cgacaccatc acgccgccgg tgatcctgcg caacgtgctc gagaacccgg
     1501 gctggtacac gcagtacacg ccgtaccagc ccgagatctc gcagggccgg ctcgaggccc
     1561 tgatcaactt ccagaccctg gtcgaggatc tcaccgcgct gccgatggcc aacgcctcgc
     1621 tgctcgacga ggcgaccgcc gccgccgagg cgatgacgat gtgcgtcgcg cacgccgcgg
     1681 agcgaggcga ccgcgacgcg cgagccgcgc gccacacgtt ctgggtccag gccgagaccc
     1741 atccgcagac gatcgccgtg atgcacaccc gcgccgagcc gctcggcgtt gcgctccggg
     1801 tcggcacggc ggccgagatc gccgcgggcc tgggccccga ggtcgccggc gtcctcctgt
     1861 cgtacccgac caccgacggc cggatcgacg atcaccgcgc gctgatcgcc cagctccacg
     1921 ccgccggggc cgcggtggtg atggcgaccg atctgctcgc gctgaccctg ctggtcccgc
     1981 cgggcgagct cggcgccgac gtcgcgatcg gctcggcgca gcggttcggc gtgccgatgg
     2041 ggttcggcgg cccgcacgcc gcgttcctcg ccgcgcgcga tgacttccgg cgccagatgc
     2101 cgggccggat catcggcgtg tcgcgcgacg ccaagggcaa gaccgcgtac cggctctcgc
     2161 tgcagacccg cgagcagcac atccgccgcg agaaggcgac ctcgaacatc tgcaccgcgc
     2221 aggtcctgct cgcggtgatg gcgtcgatgt acgcggtcta ccacggcccc gacggcctgg
     2281 tccggatcgc cgagcgcgtc cgcgggttca ccgcggtgat cgccgccggc ctggcccggc
     2341 tcggctaccg ggtccgcgcc ggggcgtact tcgacaccct gcggatcgat ctcgacgcgc
     2401 agcgccgcgc cgaggtgctc gcgcgcgcgc tcgaccgcgg gctcaacctg cggcgctacg
     2461 acgacggcgt cgggatctcg tgcgacgaga ccacgagcca ggccgacgtc cacgacctgc
     2521 tggaggcgtt cgcgcgcggc gacgagctgc cgttcgaggt cgcggcgctc gccgccgaga
     2581 ccgagctgcc gccgctcgcg ctgcgccgga gctcggcgtt cctcacccac ccgacgttcc
     2641 accgctacca ctcggagcac gagatgctcc gctacctgaa ccggctgacc accaaggacc
     2701 tgtcgctgac gacgtcgatg atcccgctgg ggtcgtgcac gatgaagctc aacgccacgg
     2761 ccgagatgct gccggtgacc tgggccgagt tcggccggct gcacccgttc gcgccgcccg
     2821 agcagtgggg cggctaccgc gcgatgttcg agcagctcga ggcctggctc gccgagatca
     2881 ccgggttcgc cgcggtgtcg ctgcagccca actccggcgc ccagggcgag tacgccgggc
     2941 tgctggcgat ccggtcgtac caccgggcgc gcggcgaggg gcatcgcacg gtctgcttga
     3001 tcccgcaatc ggcgcacggc accaacccgg cgtcggcggt gctcgcgggg atgcaggtcg
     3061 tggtcgtggc gtcggccgcc gacggcacga tcgacatggc cgacctgcgg gccaaggccg
     3121 agaagcacgc cggccagctc ggcgcgctga tggtgaccta cccgtcgacc cacggcgtgt
     3181 tcgagggcac gatccgcgag gtctgcgagg tcgtgcaccg ccacggcggc caggtctaca
     3241 tggacggcgc caacatgaac gcgcaggtcg ggctgacccg cccggcggac atcggcgccg
     3301 acgtctgtca cctcaacctg cacaagacgt tctgcatccc gcacggcggc ggcgggccgg
     3361 gcatggggcc gatcgcggtc gccaggcacc tcgcggcgta cctgccgggc cacccgctcg
     3421 ccgaacccgg gagccagccg accgggccgg tgtcggcggc gccgtggggc tcggcgtcga
     3481 tcctgccgat ctcgttcgcg tacatcgcga tgatgggcga gcccggcctg cgccgcgcga
     3541 cccaggtcgc gatcctgggc gccaactaca tcggccaccg cctgaacccg cacttcccgg
     3601 tgctgtacac cggccagaac ggccgggtcg cccacgagct gatcctcgac tgccgcggct
     3661 tcaagaagac cgccgggatc gaggccgagg acatcgccaa gcgcctgatg gactacggct
     3721 tccacgcgcc gaccat
//
LOCUS       DASVOR010000309         2460 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207429,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000309 DASVOR010000000
VERSION     DASVOR010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207429"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..643
                     /locus_tag="VF516_06430"
     CDS             203..643
                     /locus_tag="VF516_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEX8107347.1"
                     /translation="MATIPDGRLREILTTSPTVAVLGIHREQEKAAYYVPEYLHDEGY
                     RILGVNPRFRGEPLFGEPVRATLAEIGEPVDLIDVFRRPTAIPDHIEDILAMKPRPKV
                     VWLQLGIKNDEAARILEAAGITVVQNRCMLADHQRLALGAPARK"
     gene            737..988
                     /locus_tag="VF516_06435"
     CDS             737..988
                     /locus_tag="VF516_06435"
                     /inference="COORDINATES: protein motif:HMM:NF021458.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2171 domain-containing protein"
                     /protein_id="HEX8107348.1"
                     /translation="MLDSETAKGLIKPHMPVVCSNNGQFAVVDHLEGRDFIKLAKDAS
                     GQHHYIPLIWVTSVDDKVHVDRPGDQAMREWKTQPPPAA"
     gene            1002..1262
                     /locus_tag="VF516_06440"
     CDS             1002..1262
                     /locus_tag="VF516_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107349.1"
                     /translation="MSAVLQWKIENTTDATRGQPLAARVLARKAELEDALADCGRHEV
                     LRRTALETALAGVYALITGDLAHPPDVIARALNNWLERNKHL"
     gene            1377..2246
                     /locus_tag="VF516_06445"
     CDS             1377..2246
                     /locus_tag="VF516_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107350.1"
                     /translation="MLAKNTTIAGVVLGMLLAVRAHAQPDPDSETKPLDAAAAAAGGE
                     GGEQLTLQKGRLVLNAYLGINLSSGLTFKPVSLSPDLWYGVSDDLTVGLVHSFVGTTG
                     FLGGFGLSGQSLCLTGTSSGCANLYSNVGAEARYKLKMSGIAFAVDGGVYALRIQDPM
                     LLDVKLGAVGRWRKGPLAVEAAPNLLVGLTNRSVTTAGVDVTTNPDILSLPGSVLYTL
                     NPMITVAAQVGLVLPLENTGDTYTLPLSVGAFYHVNDQLDVTAAFSLPRLIAASGGGI
                     DARSLTLGGSYAF"
     gene            2236..>2460
                     /locus_tag="VF516_06450"
     CDS             2236..>2460
                     /locus_tag="VF516_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107351.1"
                     /translation="MRSRALIAVALLSSAVAGTLAGCATAEMKSACAQSFSWAAPVFR
                     CGGASGGTTPEVAVKPPGPEPEPTSGPTSGP"
BASE COUNT          417 a          848 c          850 g          345 t
ORIGIN      
        1 tgagcgcgga gcgatggcac gccgcgccga gggcggcggc cacggcgacg gcgacggcct
       61 tcgagggggc caccgagggg agcatcgagg ggagcaccga ggggagcatc gatggctcgc
      121 ctgccatgag caaaacctaa cgcgtcgctc cccggaaagc gaaccgggct ccgtcacggg
      181 ccgagcgcgg tatggtcgcg ccatggcgac gatccctgac ggccggctcc gcgagatctt
      241 gaccacgtcg ccgacggtcg cggtgctggg gatccaccgc gagcaggaga aggccgcgta
      301 ctacgtcccc gagtacctgc acgacgaggg ctaccggatc ctcggggtca acccgcggtt
      361 ccgcggcgag ccgctgttcg gcgagcccgt gcgcgccacg ctcgccgaga tcggcgagcc
      421 cgtggatctg atcgatgtat tccggcggcc caccgccatc ccggaccaca tcgaggacat
      481 cctggccatg aaaccgcgtc cgaaggtggt ctggctccag ctcgggatca agaacgacga
      541 ggccgcgcgg atcctggagg ctgcggggat caccgtggtg cagaaccggt gcatgctcgc
      601 agaccatcag cggctggcac tcggagcgcc cgctcgcaaa tagcgcgcgg tccgcgcggc
      661 ggcgcacgca gatcggccag gttctccgca attccgctcg gttgggcttg gcacgagcga
      721 tgctgcaccg gagatcatgc tggattccga gacagccaag ggcctgatca agccacacat
      781 gcccgtcgtg tgctccaaca acggccagtt cgcggtggtc gaccacctcg agggccgaga
      841 cttcatcaag ctggccaagg acgcgagtgg ccagcaccac tacatcccac tcatctgggt
      901 gacctccgtc gacgacaagg tgcacgtcga ccggccgggc gaccaggcga tgcgcgagtg
      961 gaagacccag ccgccgcctg cggcgtgatc ggagagccgc cgtgagcgcc gtcctgcaat
     1021 ggaagatcga gaacacgacc gatgcaacgc gcggtcagcc actcgccgcg cgcgtgctgg
     1081 cgcgcaaggc cgagctcgag gatgcgctcg cggactgcgg gcggcacgag gtgctgcggc
     1141 gaaccgcgct ggagaccgcg ctggccgggg tctacgccct gatcaccggc gacctcgcgc
     1201 acccgcccga cgtcatcgcc cgggccctga ataactggct ggagcgcaac aagcacctgt
     1261 agcgctccgc cagataggcc tacgggagtg gcgggtgcgt gaggccgcgc acagcgagaa
     1321 cgtcaccgcc aaaaagatgg cccggatcaa catgtgcgat attccatcgc actgtaatgc
     1381 tcgccaagaa caccacgatc gcgggggtcg tgctgggcat gttgctcgcg gtgcgcgcac
     1441 acgcccagcc cgaccctgat tccgaaacca agccactcga cgccgcggcg gccgccgccg
     1501 gtggcgaggg tggcgaacag ctgacgctgc agaaagggcg gctggtcctc aacgcatatc
     1561 tcggcatcaa cctgtccagc ggactgacgt tcaagccggt gtcgctcagc cccgacctct
     1621 ggtacggcgt cagcgacgac ctcaccgtcg gcctcgtgca cagcttcgtc ggcacgaccg
     1681 gattcctcgg tggcttcggc ctgtcgggcc agtcgctgtg cctgaccggc acgagcagcg
     1741 ggtgcgcgaa cctctacagc aacgtcgggg ccgaggctcg ctacaagctc aagatgagcg
     1801 ggatcgcgtt cgcggtcgac ggcggggtgt acgccctgcg gatccaggat ccgatgctgc
     1861 tcgacgtcaa gctcggcgcg gtcggccggt ggcgcaaggg accgctcgcg gtcgaagcgg
     1921 cgcccaacct gctcgtcggg ctcaccaacc gatcggtgac caccgccggg gtcgatgtca
     1981 cgaccaaccc cgacatcctc agcctcccgg ggagcgtgct gtacacgctc aacccgatga
     2041 tcacggtcgc cgcgcaggtc ggcctcgtgc tgccgctgga gaacaccggg gacacgtaca
     2101 cgctgccgct gtcggtcggc gcgttctatc acgtcaatga ccagctcgac gtcacggcgg
     2161 cgttcagcct accgcggctg atcgcggcca gcggaggcgg aatcgatgcg cggtcgctga
     2221 ccctcggagg aagctatgcg ttctagagcg ctgatcgctg tcgcgctgct gagcagcgcc
     2281 gtcgccggga cgctcgccgg ttgtgcgacc gccgagatga agtctgcgtg cgcgcagtcg
     2341 ttcagctggg ctgccccggt gttccgctgc ggcggcgcgt cgggcgggac cacgcccgag
     2401 gtcgcggtca agccgcccgg gcccgagccc gagccgacct cgggtccgac ctcgggcccg
//
LOCUS       DASVOR010000310         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_499748,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000310 DASVOR010000000
VERSION     DASVOR010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499748"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VF516_06455"
     CDS             <1..215
                     /locus_tag="VF516_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8107352.1"
                     /translation="ERALELAVDYAKQREQFGRPIASFQMVQAKLAEIYVRVETMRAF
                     TYRVLAACNELDASAGGRGEIHALTA"
     gene            complement(270..2528)
                     /locus_tag="VF516_06460"
     CDS             complement(270..2528)
                     /locus_tag="VF516_06460"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX8107353.1"
                     /translation="MSGSTSPNDPAVTTRLAELEATVMAQQAALEAEHAALEAERELR
                     AAAEAERDRLREAYEELKVQVELAHRRLVIAKAERVDTTQLELEFAATLAELDKLAGL
                     VEPETDATGGEGGATGDKPKRRRGRRCELHKVIGEERIEIPDPICEGKFVRIGADEEV
                     VKLKWRRGGLVRVVIARVKYRTTPPAVRTVDETPSAADVSSATSATEDAVASATMTAS
                     EDAMGSTVEVTDIDATDSANASPVDVPPAQLASSTATSDATPPEVAAAVPAAPASADP
                     TSDSIPARPTAPEDATSSAVSAASTDAAASTNASPVDVPLTQLAISTATPEAAAAVAV
                     APASVDPADDAIATRPTIVTARMPPQLLPGSYATGSLLAHIASDKFCDGLPLHRQEDR
                     FARLGVTIHRSTMARWLEEVGAIMGATIVEAMRKEALAIAFCIMTDATGVRVLPEPRP
                     DKKRQPCRHAHFFVQIADADHVFFEYTPVETSAVVRELFRGFSGFVQADAKSVYDVLY
                     RLPHERDLVDDGPPDLAVRHEVGCWSHARTKLWEAAVATQDPVAREGLARIMRMFQLE
                     RRWKDHSPDERRALRDRHLRPHVVAFFAFAEEAYERVKHQRGLLRSALGYCVRQKDAL
                     MRFLEDGRLEMTNNHSERQLRKVACGRKAWLFVGSDDHGQAAGNLLTLIASARLHKLD
                     PELYLRDVFRVLPHWPRDRYLELAPRYWSATRARLDPNELEQELGPLKVPETPLPTAT
                     SAPVPADMGPRA"
     gene            complement(2661..3110)
                     /locus_tag="VF516_06465"
     CDS             complement(2661..3110)
                     /locus_tag="VF516_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107354.1"
                     /translation="MNREQVPDQHDVARRARGDRSWRSRPRSKSEQAALEVERELRAA
                     AETERDRLREAYEALKVQVELARRRLVIAKAERVDTAQLELKFAATLLSFDGRDVRRL
                     QAARCRGIRIPEPVVTGDAARLINERVFEDPLADIDLEPVSRRHRVQ"
     gene            complement(3215..3505)
                     /locus_tag="VF516_06470"
     CDS             complement(3215..3505)
                     /locus_tag="VF516_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107355.1"
                     /translation="MVAQQAALEAERELRAAAEAERDRLQEAYEALKVQVELAHRRLV
                     IAKAERVATAQLELKFAATLLSFDGRDVRRLQAARCRGIRTRSRWSPAMRLV"
BASE COUNT          562 a         1282 c         1297 g          622 t
ORIGIN      
        1 ccgagcgcgc gctcgagctc gcggtcgact acgccaagca gcgcgagcag ttcggccggc
       61 cgatcgcgag cttccagatg gtgcaggcca agctcgccga gatctacgtg cgggtcgaga
      121 ccatgcgcgc gttcacctac cgggtgctcg cggcgtgcaa cgagctcgat gccagcgcgg
      181 gcggacgcgg cgagatccac gcgctgaccg cgtaaccggt gcaagatccc cgtcctggag
      241 ggccttgggg gggatatggt aaatggggcc tacgctctgg ggcccatgtc cgcgggcacg
      301 ggggccgatg tcgccgtagg caacggcgtt tcggggacct tcagcggacc gagctcctgc
      361 tcgagctcgt tgggatcgag gcgagcgcgt gtggcgctcc agtagcgcgg cgcgagctcg
      421 agatagcgat cgcgcggcca gtgcggcagc acgcggaaga cgtcgcgcag gtagagctcg
      481 ggatcgagct tgtgcaggcg cgcggaggcg atcaacgtga gcaggttgcc ggcagcctgt
      541 ccgtggtcat cgctaccgac aaacagccag gcttttcgac cgcaggcaac tttcctcaat
      601 tgacgctcgg aatggttgtt cgtcatctcg agacgaccat cctcgagaaa gcgcatgagc
      661 gcgtccttct ggcgcacgca gtagccgagc gccgagcgca agaggccacg ctggtgcttc
      721 acgcgctcgt atgcctcctc ggcgaacgcg aagaacgcga cgacgtgggg gcggagatgg
      781 cgatcacgca gcgccctgcg ctcgtcgggg ctgtggtcct tccagcgccg ttcgagctgg
      841 aacatgcgca tgatgcgggc gagaccctcg cgcgcgaccg gatcttgcgt cgcgacagcg
      901 gcttcccaca gcttggtcct cgcgtggctc cagcacccga cctcgtggcg aaccgcgagg
      961 tcaggtggac catcgtcgac gaggtcgcgc tcgtggggaa gtcgatacag gacgtcgtag
     1021 acgctcttgg cgtcggcctg gacgaagccg gagaagccgc ggaacagctc gcggacgacc
     1081 gcgctggtct cgacgggcgt gtactcgaag aacacgtgat cggcatcggc gatctggacg
     1141 aagaagtggg cgtggcggca tggctgacgc ttcttgtcgg gccgtggttc cggcaggacg
     1201 cgcacgccgg tggcgtcggt catgatgcag aacgcgatcg ccagcgcctc cttgcgcatc
     1261 gcctcgacga tcgtggcgcc catgatcgcg ccgacctcct cgagccagcg cgccatcgtg
     1321 ctgcggtgga tggtgacgcc gagccgtgcg aaccggtctt cctggcggtg cagcgggagg
     1381 ccatcgcaga acttgtcgct ggcaatgtgg gcgagcaacg acccagtggc gtacgagccg
     1441 ggtagcaact gcggcggcat ccgcgcggtc acgatcgtcg gtcgcgtggc aatcgcgtca
     1501 tcggccggat ccacgctcgc cggtgccacg gcaaccgccg cggcggcctc gggcgtggcc
     1561 gtcgagatcg cgagctgcgt cagcggaaca tcgaccggcg atgcgttggt cgacgccgcc
     1621 gcatcggtgc ttgctgctga gacggccgag ctggtcgcgt cctctggtgc ggtcggccgc
     1681 gcggggatcg agtcactggt cggatccgcg ctcgccggtg ctgctggaac cgccgcggcg
     1741 acctcgggcg gcgtggcatc ggacgtggcc gtcgagctcg caagctgcgc cggcggaaca
     1801 tcgaccggcg atgcgttggc cgaatccgtc gcgtcgatgt ctgtcacctc gacggtcgag
     1861 cccatcgcgt cctcagatgc cgtcatggtc gccgaagcta ccgcgtcttc ggtggcgctg
     1921 gtggctgagg acacatcggc ggccgatgga gtctcatcga ccgtgcgcac cgccggcggt
     1981 gtcgttcggt acttcacacg agcgatcacc acccggacca ggccgccgcg tcgccacttg
     2041 agcttgacga cttcctcgtc ggcgccgatg cgcacgaact tgccctcgca gatcggatcc
     2101 gggatctcga tccgctcttc gccgatcacc ttgtgcagct cgcaacgccg gccacggcgc
     2161 cgcttcggct tgtcgcccgt ggcgcctccc tcgccgccgg tcgcatcggt ctccggctcg
     2221 accaggcccg cgagcttgtc gagctcggcg agcgtagcgg cgaactccag ctccagctgg
     2281 gtcgtgtcga cgcgctcggc cttcgcgatc accagacgcc ggtgcgcgag ctcgacctgg
     2341 accttgagct cctcgtaggc ttcgcgcagt cgatcccgct cggcctcggc agcggcgcgc
     2401 agctcacgct cggcttcgag cgcggcatgc tcggcttcga gcgcggcctg ctgcgccatc
     2461 accgtcgcct cgagctcggc gagccgcgtc gtgaccgccg gatcgttcgg actcgtcgag
     2521 ccgctcacgc ttgtgcatca tgatcctgcg atcgacgcgc gcaagatgca cgagatcgga
     2581 atcgatcacc cgcgcgcgag attcctggtc cggaacttgc tcgcgatttc gtcgacggtg
     2641 gcgacgagta gcgtcgtcgg tcactgcacg cgatgacgtc gcgacaccgg ctccaggtcg
     2701 atgtccgcga gcgggtcctc gaacacgcgc tcgttgatca gacgagccgc atcgccggtg
     2761 accaccggct ccgggatccg aatgccccgg catcgagccg cttgtagacg acgcacatcc
     2821 cggccgtcga aggacagaag cgtggcggcg aacttcagct ccagctgagc cgtgtcgacg
     2881 cgctcggcct tcgcgatcac caggcgccgg cgtgcgagct cgacctggac cttgagcgcc
     2941 tcgtaggctt cccgcagtcg atcccgctcg gtctcggcgg cggcgcgcag ctcacgctcg
     3001 acttcgagcg cggcctgctc ggacttcgag cgcggcctgc tgcgccacga cctgtcgcct
     3061 cgagctcggc gagctacgtc gtgctggtcc ggaacttgct cgcgattcat cgaccgtggc
     3121 gacgagcagt gcgttggtca gtgcacgcga tgacgtcgcg ccaccggccc caggtcgatg
     3181 cccgcgagca ggtcctcgaa cgcgcgctcg ttgatcagac gagccgcatc gccggtgacc
     3241 accggctccg ggtccgaatg ccccggcatc gagctgcttg tagacgacgc acatcccggc
     3301 cgtcgaagga cagaagcgtg gcggcgaact tcagctccag ctgggccgtg gcgacgcgct
     3361 cggccttcgc gatcaccagg cgccggtgcg cgagctcgac ctggaccttg agcgcctcgt
     3421 aggcttcctg cagtcgatca cgctcggcct cggcggcggc gcgcagctca cgctcggctt
     3481 cgagcgcggc ctgctgcgcc accacccgtc gcttcaagct cggcgagctg cgtcgtgctg
     3541 gtccggaact tgctcgcgat tcatcgacgg tggcgacgag cagtgtcgtt ggtcagtgca
     3601 cgcgatgacg tcgcgacacc ggctccaggt cgatgcccgc gagcagatcc tcgaacgcgc
     3661 gctcgttgat cagacgagcc gcatcgccgg tgaccaccgg ctccgggatc cgaaatgccc
     3721 cggcatcgag ccgcttgtag atgatgcaca tcccggtgcc gtc
//
LOCUS       DASVOR010000311         4353 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207432,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000311 DASVOR010000000
VERSION     DASVOR010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4353
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207432"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..549
                     /locus_tag="VF516_06475"
     CDS             <1..549
                     /locus_tag="VF516_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107356.1"
                     /translation="GHFLSYTLSPKATFVFEKVRNASKARGAPAVDVNWPHGQRVGDP
                     VPIDLEVVADPEQFLHRATVFVRTRGEPGWRAADIALTRSTDRRIDRRILLPPIAAAG
                     PVSLELYLRAYDERGNEVLSWADPGRPREIPLRYDPPAPWYQRGWFYAAAGLALTAAV
                     GITVYELAIAPPDKITGGVSVH"
     gene            complement(577..1902)
                     /locus_tag="VF516_06480"
     CDS             complement(577..1902)
                     /locus_tag="VF516_06480"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX8107357.1"
                     /translation="MSAPTPPLSPARTGDHIRRVAIVFSGGPAPAANAVISAAATSFL
                     EDGREVIGFFHGYSNLQDYHPVTHRLLPDEHYRIFAERDVNGLRNSRGIVIGTARANP
                     GKGIERPEHLLDPERTAQLRNVYNALVDLEIHALISIGGDDTLKTANFLYEFQRRLPD
                     GARRVQIVHLPKTIDNDYRGIDFTFGFFTAVDVMAKELQNLRADAIATSSYFIVETMG
                     RKAGWLSYGVAIAGEANLVLAVEDVTGGLAVTDDGEPRLSLDALVERIVDLIITRERR
                     GKHYGTVVLAEGLAEMLPPAQTKDLPRDEHGHLSLGRIDLGKLVAQKVMKRYQARTGR
                     KKKATGLQLGYESRCAPPHAFDVMLGSQLGIGAYRALVEENLDGHMVSIEGQLDLRYV
                     PFKELINPQTLKTEVRFMPAGGDYHRLARFLETRTDKLVEWSPGRRVER"
     gene            1992..3386
                     /locus_tag="VF516_06485"
     CDS             1992..3386
                     /locus_tag="VF516_06485"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8107358.1"
                     /translation="MAMTLRRIATLGLMMFGPAGGHAAPATQADVKTMLRGISDKRIE
                     ATVRKLAGLGTRHTLSDTASDARGVGAARRWIKAQLDACAQQSGGRLKVELDSFTAQP
                     GRRIPKPTELVNVIATLPGDQAESVDRIYVVSGHYDSMPSNVMDPAADAPGADDDASG
                     TAAVIEIACAMSKLHFDATLVFMTVAGEEQGLVGSTHVADEARKANKNIAGMFTNDIV
                     GNSRAEDGRVDRGRVRLFAEGVPDVKEMSDELRTLVRTGGENDTSTRELARFIKEHAE
                     RDVAGIKVDLVWRRDRYLRGGDHAPFLNAGYAAVRFTEPAENYKHQHQTPRVEGGVQY
                     GDLPEFVDFAYIAQVARVNAAALAALALAPAAPRGVQMETVKLENDTTLRWDANPEPD
                     LAGYRIVWRDTTAPLWQNHKDVGLVTHATLPLSKDNVVFGVQAIDKDGNVSVASYPTP
                     LQPPRPAPAPPAKP"
     gene            3715..4311
                     /locus_tag="VF516_06490"
     CDS             3715..4311
                     /locus_tag="VF516_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107359.1"
                     /translation="MFYIADRKTLTSKVQAPFVLAEIRALRSSIFKNTMEVQQALSVA
                     AVVEQKYDLTRTDLFLPVLDTFCAILDPLSGTLLRGTLRRVGNEIFPALISVLGIPPS
                     NVKVAMGLKEPPDLIRAICGYYSQCVIGPEAGSLAPMITHGGVSVTDTSMMPCQLQMG
                     VFVGAGTMTGMFRDSSLVEKRCRAKGDPSCVYEFTFSY"
BASE COUNT          638 a         1500 c         1565 g          650 t
ORIGIN      
        1 gggcatttcc tgagctacac gctgtcgccc aaggcgacgt tcgtgttcga gaaggtgcgc
       61 aacgcgagca aggcgcgcgg cgcgccggcg gtcgacgtca actggccgca cggccagcgg
      121 gtcggcgatc cggtgccgat cgacctcgag gtcgtcgccg atcccgagca gttcctgcac
      181 cgcgcgacgg tgttcgtccg cacccgcggc gagcccggct ggcgcgccgc cgacatcgcg
      241 ctgacccgga gcaccgaccg ccggatcgat cgccggatcc tgctgccgcc gatcgcggcc
      301 gcggggccgg tgtcgctcga gctctacctg cgcgcctacg acgagcgcgg caacgaggtg
      361 ctgagctggg ccgacccggg gcggccgcgc gagatcccgc tgcgctacga tccgccggcg
      421 ccgtggtacc agcgcggctg gttctacgcc gcggctggcc tcgcgctcac cgcggccgtc
      481 gggatcaccg tgtacgagct cgcgatcgcg ccgcccgaca agatcaccgg cggcgtgtcg
      541 gttcattgaa gcgccgaacg tcggccgaac gtgggatcaa cgctcgaccc ggcggcccgg
      601 gctccactcg accagcttgt cggtgcgggt ctcgaggaac cgggcgagcc ggtggtagtc
      661 gccgcccgcg ggcatgaacc ggacctcggt cttgagggtc tgggggttga tcagctcctt
      721 gaacggcacg tagcgcaggt cgagctggcc ctcgatgctg accatgtggc cgtcgaggtt
      781 ctcctcgacc agcgcgcggt aggcgccgat gccgagctgg ctgccgagca tgacgtcgaa
      841 cgcgtgcggc ggcgcgcagc gcgactcgta gccgagctgc aggccggtcg ccttcttctt
      901 gcgcccggtc ctggcctggt agcgcttcat caccttctgg gcgaccagct tgccgaggtc
      961 gatccggccg agcgacaggt ggccgtgctc gtcgcgcggc aggtccttgg tctgggcggg
     1021 gggcagcatc tcggcgaggc cctcggcgag cacgaccgtg ccgtagtgct tgccgcggcg
     1081 ctcgcgggtg atgatgagat cgacgatgcg ctcgaccagc gcgtcgagcg acagccgggg
     1141 ctcgccgtcg tcggtcaccg ccagcccgcc ggtgacgtcc tcgaccgcga gcacgaggtt
     1201 ggcctcgccg gcgatcgcca cgccgtacga cagccagccg gccttgcggc ccatggtctc
     1261 gacgatgaag tagctcgacg tggcgatcgc gtcggcgcgc aggttctgca gctccttggc
     1321 catcacgtcg actgcggtga agaacccgaa cgtgaagtcg atgccgcggt agtcgttgtc
     1381 gatcgtcttg ggcaggtgga cgatctggac ccggcgcgcg ccgtcgggca gccggcgctg
     1441 gaactcgtag aggaagttgg cggtcttgag cgtgtcgtcg ccgccgatcg agatcagcgc
     1501 gtggatctcg aggtcgacca gcgcgttgta gacgttgcgc agctgcgcgg tgcgctcggg
     1561 gtcgagcagg tgctccgggc gctcgatgcc cttgcccggg ttggcccgcg cggtgccgat
     1621 cacgatgccg cgcgagttgc gcagcccgtt gacgtcgcgc tcggcgaaga tccggtagtg
     1681 ctcgtcgggc aacagccggt gggtcaccgg gtggtagtcc tggaggttcg agtagccgtg
     1741 gaagaacccg atcacctcgc ggccatcctc caggaacgag gtcgccgccg ccgagatcac
     1801 ggcgttggcc gcgggggcgg gcccgcccga gaacacgatc gcgacgcgcc ggatgtgatc
     1861 gcccgtgcgg gcagggctga ggggcggggt gggggcggac atctggggcg cagcgtactc
     1921 cgcgccgggg tcgcggcaac accgcgccag cccgcgcgcc ggctgtcccg gtgatgtacc
     1981 atcgccgcgc catggcgatg accctccgac ggatcgcgac gctcggcctg atgatgttcg
     2041 gcccggcggg tggccacgcc gcgcctgcga cgcaggccga cgtcaagacc atgctgcgag
     2101 ggatctcgga caagcggatc gaggcgacgg tgcgcaagct cgccgggctc ggcacgcggc
     2161 acacgctgtc ggacaccgcg agcgacgcgc gcggggtcgg cgcggcgcgg cgctggatca
     2221 aggcgcagct cgacgcctgc gcccagcagt ccggcggccg gctcaaggtc gagctcgaca
     2281 gcttcaccgc gcagcccggc cggcggatcc ccaagccgac cgagctggtc aacgtgatcg
     2341 ccacgctgcc cggcgaccag gccgagtcgg tcgaccggat ctacgtggtc agcggccact
     2401 acgactcgat gccgtcgaac gtgatggacc cggcggccga cgcgcccggc gccgacgacg
     2461 acgcctcggg caccgccgcg gtgatcgaga tcgcgtgcgc gatgtcgaag ctgcacttcg
     2521 acgccacgct ggtgttcatg acggtcgccg gcgaggagca gggcctggtc ggctcgaccc
     2581 acgtcgccga cgaggcccgc aaggcgaaca agaacatcgc cgggatgttc accaacgaca
     2641 tcgtcggcaa ctcgcgggcc gaggacggcc gggtcgaccg cggccgggtc cggctgttcg
     2701 ccgagggcgt gcccgacgtc aaggagatgt ccgacgagct gcgcacgctg gtccggaccg
     2761 gcggcgagaa cgacacctcg acccgcgagc tcgcgcgctt catcaaggag cacgccgagc
     2821 gcgacgtcgc cggcatcaag gtcgacctgg tgtggcgccg cgatcgctac ctgcgcggcg
     2881 gcgaccacgc gccgttcctc aacgccggct acgccgcggt gcggttcacc gagccggcgg
     2941 agaactacaa gcaccagcac cagaccccgc gcgtcgaggg cggcgtccag tacggcgacc
     3001 tgccggagtt cgtcgacttc gcgtacatcg cccaggtcgc gcgggtcaac gccgcggcgc
     3061 tcgcggcgct cgcgctggcc ccggccgcgc cgcgcggcgt ccagatggag accgtcaagc
     3121 tggagaacga caccacgctg cgctgggacg ccaaccccga gcccgatctg gccgggtatc
     3181 gcatcgtctg gcgcgacacc acggcgccgc tgtggcagaa ccacaaggac gtcggcctgg
     3241 tcacccacgc cacgctgccg ctgtccaagg acaacgtcgt gttcggcgtg caggcgatcg
     3301 acaaggacgg caacgtcagc gtcgcgagct accccacgcc gctgcagccg ccgaggcccg
     3361 ccccggcgcc gccggccaag ccgtgatttg cgacgcgacc ggtcatgtcc gggatccgga
     3421 caggtcgacg acccgcggct cgggccaccg ccgcctgccg tctcggagag aacgccctct
     3481 cgcttcgatg cggtccgagt tccgtttcct ggacaacagt ctgcggccag ggtgttccgc
     3541 gtgcgggttg tgcgccggct gaatattcca gtggcaacgc aagcgacgtg accgcgggcc
     3601 gccgcggtcc aacggtggat cggcgtgcgt gttttccgtc gatcgaagcg cccgggtccg
     3661 gccttccgtc ccgctaccgg atcaggtaca aggacttcag aacggagcgc tcccatgttc
     3721 tacattgcag atcggaagac gctgacctcg aaggtgcaag cgcccttcgt gctggcggag
     3781 atccgggcgc tgcgctcgtc gatcttcaag aacacgatgg aggtccagca ggcgctctcc
     3841 gtcgcggcgg tcgtcgagca gaagtacgat ctcacgcgga cggacctgtt tctcccggtg
     3901 ctcgatacgt tctgcgcgat cctcgatccg ctgtcgggga cgctgctgcg cggcacgctg
     3961 cggcgggtcg gcaacgagat cttcccggcg ctgatctccg tcctggggat tccgccgtcg
     4021 aacgtgaagg tcgcgatggg gctcaaggag ccgccggacc tgatccgggc gatctgtggc
     4081 tactactcac agtgcgtgat cgggcccgag gccggctctc tggcaccgat gatcacgcat
     4141 ggcggggtca gcgtgaccga tacgtcgatg atgccctgcc agctccagat gggcgtgttc
     4201 gtcggcgcgg gcacgatgac cggcatgttc cgggatagct cgctggtcga gaagcgctgc
     4261 cgggccaagg gcgatccgtc ctgcgtgtac gagttcacgt tctcgtactg agcacggcca
     4321 ccggctcggc cctgatccgt gcatgatgcg ggc
//
LOCUS       DASVOR010000312         7711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207449,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000312 DASVOR010000000
VERSION     DASVOR010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207449"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /gene="tnpB"
                     /locus_tag="VF516_06495"
     CDS             <1..268
                     /gene="tnpB"
                     /locus_tag="VF516_06495"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX8107360.1"
                     /translation="ALIRERMRAEPRSRALFVFVGKRGHSMKVLTWDGTGTIVVHKKL
                     DAGKFELPRPTRSGEQHLLVSDAIFEAIHKGVAITPRAAPRRVH"
     gene            324..2030
                     /locus_tag="VF516_06500"
     CDS             324..2030
                     /locus_tag="VF516_06500"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX8107361.1"
                     /translation="MASPPSDSSPVTMAAEDLATQLAALQAELVAIKAESTAKVASLE
                     ETIGNLAHENALLKRRLYGNKTERSRTSELQLALGEMLAGEAQLQGELDAAVGKAKDA
                     IEPEPKPAPGPRATPKGRRDLSVSKLPSVLVEILDEEREASGCRRIGFEDSRQLMFRR
                     GGFAILVKRVAKYEVIRDGETTVEAVPSPETLFPRALLHTSAIAHVVTSKFALGVPHY
                     RLEQDLADQGVSLDRGTMSRYVEHVGSTLGATIVHAMWQDALANAQVISTDATGALIQ
                     PAKTKDGRPQACKKGHFFTAVVDCDAVLFAYAEQHTSAFVQSLFGTFRGCLQADASSV
                     YNILEHGPPKDSEDGVRLLGCFAHLRRYFFEAAICRYPVGLQGLLRIRAIYAADHVVQ
                     RAPAAERKALRDTHVRPLVDDFFTWVHAARAVTPGRNLATKALGYAANQEAELRRIFD
                     EADLPLDNTRAERALRKIVVGRKSWMFYGSDTHAEAAAAIFSVIASCRLHGLDPFQYL
                     EEILRLLPYWPRNRYLELAPKYWRATRGRLRPDELASPISAFEVPPPLDETVDLVAAP
                     NA"
     gene            2223..4142
                     /locus_tag="VF516_06505"
     CDS             2223..4142
                     /locus_tag="VF516_06505"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8107362.1"
                     /translation="MRSIVLAGIAGLIACASPAKLARPAVIALGDATVAAPPRWYVEQ
                     RADRVTFMDPDRTLHVTIVANAGADAATAIATAWQLAEPGFALTPGEPDEMPDSGDWD
                     AVTTIEYRPPTSEHRTVIARWRKSGTHRFVVLVDGDRAAIDRREADVKVLEGSLHVPG
                     MREQTLGARHPFDARQLDAFVQRALTELVVPGAAIAVIDHGKVVYERTLGVRSLGDPA
                     PVTVGTRFLIASITKPMTTLMEAALVDAGLVDWETRVVSVLPTFAIGDPEVTRELRLW
                     HMSCACTGMPRQDLEDLFEWDGVTPEARLAAMRTMKPTTKLGETFQYSNLMVAAGGFI
                     AAHAFAPERPLAEAYAAAMQAKVFGPIGMTSTTLDYTAVAGGDHAVPHALAIDGTTRA
                     MPLAIERGVEPIAPAGGVWTTVRDMERYVATELGDGVTPEGVRVVSATNMRERLRVRV
                     RSSATGGYGLGIGVGTYSGTRVIAHDGGSMGFGTTMFTLPTEHIGIIIFTNIRNAKEE
                     LPFNAAVTRRIVELLFDAAVPLANKKLAFYVKLRHKKPHVASPDHGWVAPLVGTYHDA
                     ALGDVVIRATAAGADLDAGEWRVAIDQEVEPDGTVKLVVLDPPFAGSAWVVGPGPSLL
                     IPGYPGYRFTRKPPS"
     gene            complement(4700..5473)
                     /locus_tag="VF516_06510"
     CDS             complement(4700..5473)
                     /locus_tag="VF516_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8107363.1"
                     /translation="MVWGLSEQEIFDEVIVTPRRAFPPLPDLSVIERPGWMQIITPSI
                     KTGSLNEVLFSALDADGADVVIDAAIAEYRRLGLKFRWSVGPGSAPADLGERLARRGL
                     VASWGRGMAWSTSALPQADDPAIRIVEVDAMTIDAFTQVTARGWDLELTPTAELHAQI
                     LAAPGRKQHLFLAYHRGEPAATASYFAFPRSAYLLGGVVLPQHRGRGLYRALVAARLG
                     HARARGISLATTHARESSSAPILERLGFATICRFPRYFS"
     gene            complement(5636..5764)
                     /locus_tag="VF516_06515"
     CDS             complement(5636..5764)
                     /locus_tag="VF516_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107364.1"
                     /translation="MWGDTAGTKQLDAEVGWLTIPELPLPTMLPSDALAAPPSPAP"
     gene            complement(5899..7218)
                     /locus_tag="VF516_06520"
     CDS             complement(5899..7218)
                     /locus_tag="VF516_06520"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX8107365.1"
                     /translation="MVEILDEEREANGCRRIGFEDSQQLMFRRGGFAILVKRVAKYEV
                     IRDGETTVEAVPSPETLFPRALLHTSAIAHVVTSKFALGVPHYRLEQDLGDQGVSLDR
                     GTMSRYVEHAGGTLGATIVHAMWQDALTNAQVISTDATGALIQPAKTKDGRPQACKKG
                     HFFTAVVDCDAVLFAYSEQHTSAFVQSLFGTFRGCLQADASSVYNILEHGPPKDSEDG
                     VRLLGCWAHCRRYFFEAAICRYPVGLQGLLRIRAIYAADHVVQRAPAAERKALRDTHV
                     RPLVDDFFTWVHAARAITPGRNLATKALGYAANQEVELRRIFDEGDLPLDNTRAERAL
                     RKIVVGRKSWMFYGSDTHAEAAAAIFSVIASCRLHGLDPFQYLEEILRILPYWPRDRY
                     LELAPKYWRATRGRLGPDELASPISAFEVPPPLRESVEVAAAPAPIA"
     gene            complement(7143..7610)
                     /locus_tag="VF516_06525"
     CDS             complement(7143..7610)
                     /locus_tag="VF516_06525"
                     /inference="COORDINATES: protein motif:HMM:NF024408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8107366.1"
                     /translation="MASPPSDSSPATMAAEDLATQLAALQAELVAIKAESTAKVASLE
                     ETIGNLAHENALLKRRLYGNKTERSRTSELQLALGELLAGETQLQGELDAAVGKAKDA
                     IEPEPKPAPGPRATPKVAATSASASCRASWSRSSTKSAKRTGAGGSGSRTASS"
BASE COUNT         1186 a         2689 c         2669 g         1167 t
ORIGIN      
        1 ggcgctgatc cgcgagcgga tgcgggccga gccgcgctct cgcgcgctgt tcgtgttcgt
       61 gggcaagcgc ggccacagca tgaaggtcct gacctgggat ggcaccggga cgatcgtcgt
      121 tcacaagaag ctcgacgccg ggaagttcga gctaccgcgg ccgacgaggt ccggcgagca
      181 acacctcctg gtgagcgatg cgatcttcga ggcgatccac aagggcgtcg cgatcacccc
      241 gcgcgctgca ccacgacgcg tccactgatc gacgacgtcg ttctcgcttg cgcgatcgat
      301 ccgcgctgcg tataggtgga ttcgtggcga gcccaccgtc ggattcgagc ccggtcacga
      361 tggccgcgga ggatctcgcg acgcagctcg ccgcgctcca ggctgagctg gtggcgatca
      421 aggccgagtc gacggcgaag gtcgcctcgc tggaggagac gatcggcaac ctggcgcacg
      481 aaaacgcgct gctcaagcgg cggctgtacg gcaacaagac cgagcgcagc cggaccagcg
      541 agctgcagct cgcactgggc gagatgctcg ctggtgaggc gcagctgcag ggcgagctcg
      601 acgccgcggt cggcaaggcc aaggacgcga tcgagcccga gcccaagccg gcgccagggc
      661 cgcgcgccac gcccaagggt cgccgcgatc tcagcgtcag caagctgccg agcgtcctgg
      721 tcgaaatcct cgacgaagag cgcgaagcga gcggctgccg gcggatcggg ttcgaggaca
      781 gccggcagct gatgttccgg cgcggcggct tcgcgatcct ggtcaagcgg gtcgccaagt
      841 acgaggtgat ccgcgacggg gaaacgacgg tggaggccgt gcccagcccc gagaccctgt
      901 ttccgcgggc gctgcttcac acgtcggcga tcgcgcacgt cgtcacgtcg aagttcgcgc
      961 tcggcgtgcc gcactatcga ctcgagcagg acctggccga ccaaggcgtc tcgctcgacc
     1021 gcgggacgat gagccgttac gtcgagcacg tcggcagcac gctcggcgcg acgatcgtcc
     1081 acgcgatgtg gcaggacgcg ctggccaacg cgcaggtgat ctcgaccgac gcgaccggcg
     1141 cgctgatcca gccggcgaag accaaggacg gtcgcccgca ggcgtgcaag aagggccact
     1201 tcttcaccgc cgtcgtcgac tgcgacgcgg ttctgttcgc gtacgccgag cagcacacca
     1261 gcgcgttcgt gcagagcctg ttcggcacgt tccgcggctg cctgcaggcc gatgcgagca
     1321 gcgtctacaa catcctcgag cacggcccgc cgaaggacag cgaagacggt gtgcggctcc
     1381 taggctgctt cgcgcacctc cgccggtact tcttcgaggc ggccatctgt cgctacccgg
     1441 tcggcctgca gggcctgttg cggatccgtg cgatctacgc cgcggatcac gtcgtacagc
     1501 gcgcgccggc cgccgagcgc aaggcgctcc gcgacacgca cgtccgtccg ctcgtcgacg
     1561 atttcttcac ctgggtgcac gcggcgagag ccgtcacgcc cgggcgcaat ctggcgacca
     1621 aggcgctcgg ctacgcggcg aaccaagaag ctgagctgcg acgcatcttc gacgaggctg
     1681 acctaccgct cgacaacaca cgagcggagc gcgcgcttcg aaagatcgtc gtcggtcgta
     1741 agagctggat gttttacggc agcgacacgc acgccgaggc cgccgcggcg atcttcagcg
     1801 tgatcgcctc gtgccggctg catggcctcg atccattcca gtacctcgag gagatcctgc
     1861 gcctcttgcc atactggcct cgaaaccgct acctcgagct cgcacccaag tactggcgcg
     1921 cgacgcgcgg ccggctcaga cccgacgagc tcgcctcgcc gatctccgcg ttcgaggtgc
     1981 cgccgccgct cgacgagacc gtcgacttgg tcgcggcgcc gaacgcgtag ccgcgaccgc
     2041 agtcgtgtgg ttggtccagc tgcgcccgtc ggcagtgccc gagctcggcg acccggtgag
     2101 tcgggtcgag ccgcgctgtg tcgatctggc ccgcgcgctc gacgcgacgc ccaatgcgca
     2161 acggtagact gcgagctgtg tcggctgccg caaccgagct agcgatcatg tgtctctcgc
     2221 gcgtgagatc gatcgtcctc gccggcatcg ccggcctcat cgcatgcgcc tcccccgcga
     2281 aactcgcgcg accggccgtg atcgcgctcg gcgacgccac ggtagcggca ccgccgcgct
     2341 ggtacgtcga acagcgcgcc gatcgcgtga cgttcatgga tccggatcgc acgctgcacg
     2401 tgacgatcgt cgcgaacgcg ggcgcggatg cggcaaccgc gatcgcgacc gcatggcagc
     2461 tcgccgagcc aggcttcgcg ctcacgccgg gcgagcccga cgagatgccc gacagcggcg
     2521 actgggacgc ggtgacgacg atcgagtaca ggccgccgac gtcggagcat cgcaccgtga
     2581 tcgcgcggtg gcgcaagtca ggcacgcatc gcttcgtcgt cctcgtggat ggcgaccgcg
     2641 cggccatcga ccggcgcgag gcagatgtca aggtgctcga gggcagcctc cacgtgcccg
     2701 gcatgcgcga gcagacgctc ggcgcgcgcc acccgttcga cgcgcgccag ctcgacgcct
     2761 tcgtccagcg ggcgctgacc gagctcgtgg tgcccggcgc cgcgatcgcg gtgatcgatc
     2821 acggcaaggt tgtctacgag cgtacgctcg gcgtgcgctc actgggcgat ccggcgccgg
     2881 tcacggtcgg tacgcgcttc ctcatcgcat cgatcaccaa gcccatgacc acgctcatgg
     2941 aggccgcgct cgtcgatgcg ggacttgtgg actgggaaac gcgggtcgtg tccgtgctgc
     3001 ccacgttcgc gatcggcgat ccggaggtca cgcgcgagct gcggctgtgg cacatgtcgt
     3061 gcgcgtgcac gggcatgccg cgtcaagatc tcgaagatct gttcgagtgg gacggtgtga
     3121 cgcccgaggc ccggctcgcc gcgatgcgga cgatgaagcc gaccacgaag ctcggcgaga
     3181 ccttccagta ctcgaacctg atggtcgccg ccggcggctt catcgcggcg catgcgttcg
     3241 cgcctgagcg cccactcgcc gaggcgtatg cggctgcgat gcaggccaag gtgttcggtc
     3301 cgatcggcat gacgtcgacc acgctcgatt acaccgcggt ggcgggcggc gatcacgctg
     3361 tgccgcacgc gctcgcgatc gacggcacga cgcgcgcgat gccactcgcg atcgagcgcg
     3421 gcgtcgagcc gatcgcaccc gccggcggcg tgtggacgac cgtacgcgac atggagcgct
     3481 acgtcgccac cgagctcggc gatggcgtga cccccgaagg cgtgcgcgtg gtatcggcga
     3541 cgaacatgcg cgagcgcctg cgggtgcgcg tccgctcgag cgcgaccggc ggctacgggc
     3601 tcggcatcgg ggtcggtacg tactccggca cccgcgtgat cgcgcacgac ggcggctcga
     3661 tgggcttcgg cacgacgatg ttcacgctgc ccacggagca catcggcatc atcatcttca
     3721 cgaacatccg caacgccaag gaggagctgc cgttcaatgc ggccgtgacc cgccgcatcg
     3781 tcgagcttct gttcgacgca gcggtaccgc tcgcgaacaa gaagctcgcg ttctacgtca
     3841 agcttcgcca caagaagccg cacgtggcct cgccggacca cggctgggtg gcgccgctcg
     3901 tcggcacgta ccacgacgcc gcactcggtg acgtcgtgat tcgcgccacc gcggccggcg
     3961 cagacctcga cgcgggcgag tggcgcgtcg caatcgacca ggaggttgag ccagacggta
     4021 cggtgaagct cgtcgtgctc gatccaccgt tcgccggcag cgcgtgggtc gtcggccccg
     4081 gcccgtcgct gctgattccg ggatatccgg gctacaggtt cacgaggaag ccgccgagtt
     4141 gatcgccacg gcacggttct ctttcgtgtc cgacatgaac ccgcgatcgt cgggctcgcg
     4201 tgtcaggcca caagggtggg gtttgcgaag cgcctacgga ctgccgatct acgtggcgct
     4261 cgagcgcaac gcgaggccgg ctcagacccg acgagctcac ctcgccgatc tccgcgttcg
     4321 aggtgccgcc gccgcttcgc gagtccggtc gagttgacca ctgcgccagc gccgaccgcg
     4381 tagccgcgca cgcccgtcac gctcggcggt gcgagtgatc gcgcgcggcc gacgtggccc
     4441 ccgcgcgctc ggccagcgga gccaagtggg caggctccgg cacgtttgca tgatctgcgt
     4501 gccagcggga gccttcaatg tgggagcgat gcagcgggta cgaacgtacc ggagcctgcc
     4561 gcctcgaaag agggcgcgcg aacgacgcgc ggcgggccca gcacagcgac tcggctgccg
     4621 ccgagcggcg ttggttccgc cagctccggg cgagttctta tgcgcatatc gtcttcctcg
     4681 cggccacgct cgagttcgat cagctgaagt acctcgggaa ccggcagatc gtcgcgaagc
     4741 cgaggcgctc gaggatcggc gccgagctcg actcgcgggc gtgcgtggtc gcgagcgaga
     4801 tgccgcgcgc ccgcgcgtga ccgagccgcg cggcgaccag cgcgcggtac aggccgcggc
     4861 cgcggtgctg cggcagcacc accccgccga gcagatacgc cgagcgcgga aacgcgaagt
     4921 agctcgccgt cgccgccggc tcgccgcggt ggtacgcgag gaacaggtgc tgcttccgcc
     4981 cgggcgccgc gaggatctgc gcatgcagct cggcggtcgg cgtcagctcc aagtcccagc
     5041 cgcgcgcggt cacctgcgtg aacgcatcga tcgtcatcgc gtcgacctcg acgatccgga
     5101 tcgccggatc gtccgcctgc ggtagcgcgc tggtcgacca cgccatcccg cgcccccagc
     5161 tcgcgacgag cccgcgccgc gcgagccgct cgccgagatc cgccggcgcg ctgcccgggc
     5221 cgacgctcca ccggaacttg agcccgagcc ggcggtactc ggcgatcgcc gcgtcgatga
     5281 ccacatcggc cccgtccgca tcgagcgccg agaacagcac ctcgttgagg ctgccggtct
     5341 tgatcgacgg cgtgatgatc tgcatccagc ccggccgctc gatcaccgag aggtcgggca
     5401 gtggtggaaa cgcgcgcctc ggcgtcacga tcacctcatc gaagatctcc tgctcggaca
     5461 gcccccacac catccccgca gcgtagcagc ttcgcgccac gcgacatcgc ggtccgccgg
     5521 ctcgtggacg ccggcgtcga gccaatcggg tggccaaggg ctgctggcgc tcgggtgatc
     5581 cgtaagcgct tcgcgagccc cgtccttgac ggcggcggtg gtaggctgtc cgcgcctacg
     5641 gcgcgggtga gggcggcgcc gcgagcgcgt cgctcggcag catcgtcggt agcggcagct
     5701 cggggatcgt gagccagccg acctcggcgt cgagttgctt ggtacccgct gtatcgcccc
     5761 acattgaagg ctccccctgg cactcagacc atgcaaacgt gccggaggct gcctgcttgg
     5821 ctccgctggc cggagcgcgc gggagccacg ggcgctgcgc gcgatcacct gcatcgcgag
     5881 cgcggcgggc tcgcgcggct acgcgatcgg cgcgggcgca gcagccacct cgacggactc
     5941 gcgaagcggc ggcggcacct cgaacgcgga gatcggcgag gcgagctcgt cgggtccgag
     6001 ccggccgcgc gtcgcgcgcc agtacttggg tgcgagctcg aggtaacgat ctcgaggcca
     6061 gtacggcaag atgcggagga tctcctcgag gtactggaag ggatcgaggc catgcagccg
     6121 acacgaggcg atcacgctga agatcgccgc ggccgcctcg gcgtgcgtgt cgctgccgta
     6181 aaacatccag ctcttacgac cgacgacgat ctttcgtagc gcgcgctccg ctcgcgtgtt
     6241 gtcgagtggt aggtcaccct cgtcgaagat gcgtcgcagc tcgacctcct ggttcgccgc
     6301 gtagccgagc gccttggtcg ccaggttgcg accgggcgtg atggctctcg ccgcgtgcac
     6361 ccaggtgaag aagtcgtcga cgagtggacg gacgtgcgtg tcgcggagcg ccttgcgctc
     6421 ggcggccggt gcgcgttgca cgacgtggtc cgcggcgtag atcgcacgga tccgcaacag
     6481 gccctgcagg ccgacgggat agcgacagat ggccgcctcg aagaaatacc gtcggcaatg
     6541 cgcccaacaa cccagaagcc gcacgccatc ctcgctgtcc tttggcgggc cgtgctcgag
     6601 gatgttgtag acgctgctcg cgtcagcctg caggcagcca cggaacgtgc cgaacaggct
     6661 ctgcacgaac gcgctggtgt gctgctcgga gtacgcgaac agaaccgcgt cgcagtcgac
     6721 gaccgcggtg aagaagtggc ccttcttgca cgcctgcggg cgaccgtcct tggtcttcgc
     6781 cggctggatc agtgccccgg ttgcgtcggt cgagatcacc tgcgcgttgg tcagcgcgtc
     6841 ctgccacatc gcgtggacga tcgtcgcgcc gagcgtaccg ccggcgtgtt cgacgtaacg
     6901 gctcatcgtc ccgcggtcga gggagactcc ttggtcgccc aggtcctgct cgagtcgata
     6961 gtgcggcacg ccgagcgcga acttcgacgt gacgacgtgc gcgatcgccg acgtgtgcag
     7021 cagcgctcgc ggaaacaggg tctcggggct gggcaccgcc tccaccgtcg tctccccgtc
     7081 gcggatcacc tcgtacttgg cgactcgctt gaccaggatc gcgaagccgc cgcgccggaa
     7141 catcagctgc tggctgtcct cgaacccgat ccgccggcac ccgttcgctt cgcgctcttc
     7201 gtcgaggatc tcgaccaaga cgcgcggcag cttgctgacg ctgaggtcgc ggcgaccttg
     7261 ggcgtggcgc gcggtcctgg cgcaggcttg ggctcgggct cgatcgcgtc cttggccttg
     7321 ccgaccgcgg cgtcgagctc gccctgcagc tgcgtctcgc cggcgagcag ctcacccagt
     7381 gcgagctgca gctcgctggt ccggctgcgc tcggtcttgt tgccgtacag ccgccgcttg
     7441 agcagcgcgt tctcgtgcgc cagattgccg atcgtctcct ccagcgaggc gaccttcgcc
     7501 gtcgactcgg ccttgatcgc caccagctcg gcctggagtg cggcgagctg cgttgcgaga
     7561 tcctccgcgg ccatcgtggc cgggctcgaa tccgacggtg ggctcgccac gaatccacct
     7621 atacgcagcg cggatcgatc gcgcaagcga gaacgtcgtc gtccatcaat ggacgcgacg
     7681 tggcgaggca cgcggcgtga tggcgacgcc c
//
LOCUS       DASVOR010000313         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_178311,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000313 DASVOR010000000
VERSION     DASVOR010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178311"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..437
                     /locus_tag="VF516_06530"
     CDS             <1..437
                     /locus_tag="VF516_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107367.1"
                     /translation="FGLVVAARATPARADVGLGVFLGEPTGLDLKIGLDHRSGLDIVL
                     GFTRLSSNADGYGHVTYLVTPVVAQGDAVTVPLRVGIGGALFGTRNDLEVAIRAPLEV
                     GFRFRRTPLELYGEIALAFVFVDPANELELDVQGGGGFRVYF"
     gene            complement(441..1517)
                     /locus_tag="VF516_06535"
     CDS             complement(441..1517)
                     /locus_tag="VF516_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107368.1"
                     /translation="MVVGVVVGVVAAILSLTIGCSSEPDPPAGSMTMDLAIAPAVTID
                     TVSWTISNAAAGFSRTGSVAVRFSNVIAFQAGALPAASGYTIALSAASVDGAFSCSGS
                     AGFSISAFAITPVGLQLNCSAMAPGQGTVVVTGTTQICATLTALEASPLETSVNAPIA
                     LSATGSAGTLPVSYSWTATAGTLDNPQSETPTFTCPATPGPVKISVTVSPSAPACSTV
                     TSQSVTVTCSALAPTFTNVYTTIIGQRCIDCHHPGGVGVRVGMLDMSSQAVAFANLVG
                     VNAQGTGAGTSGNTCASVTPALVRVVPGDPNSSLVFNKVHGKLAGTLAPCGSPMPLPA
                     AAAALTQAEVDLISAWITAGAPNN"
BASE COUNT          260 a          651 c          618 g          269 t
ORIGIN      
        1 ttttcgggct ggtggtcgcg gcgcgggcaa ctccggcgcg ggccgacgtc ggtctcggtg
       61 tttttctggg ggagccgacc gggctcgacc tcaagatcgg cctcgaccac cgctcggggc
      121 tcgacatcgt cctcggcttc acccggctca gcagcaacgc cgacggctac ggccacgtga
      181 cctacctggt cacgccggtg gtcgcccagg gcgacgccgt caccgtgccg ctccgcgtcg
      241 gcatcggcgg agccctgttc ggcacccgca acgatctcga ggtcgcgatc cgcgcgccgc
      301 tcgaggtcgg cttccgattt cgccgcacgc cgctcgagct ctacggcgag atcgcgctcg
      361 ccttcgtgtt cgtcgacccc gccaacgagc tcgagctcga cgtccagggc ggcggcgggt
      421 tccgcgtgta cttctagcga tcagttgttc ggcgcgccgg cggtgatcca cgccgagatc
      481 agatcgacct cggcctgggt cagcgccgcc gcggcggcgg gcagcggcat cggcgagccg
      541 cacggcgcga gcgtcccggc gagcttgccg tggaccttgt tgaacaccag gctgctgttc
      601 gggtcgccgg gcacgacccg gaccagcgcc ggcgtgaccg acgcacacgt gttgccggac
      661 gttccggccc cggtgccctg cgcgttgacg ccgaccaggt tcgcgaacgc cacggcctgc
      721 gagctcatgt cgagcatgcc gacccggacg ccgacgccgc ccgggtggtg gcaatcgatg
      781 cagcgctggc cgatgatcgt ggtgtagacg ttggtgaacg tcggagcgag cgcgctgcag
      841 gtcacggtca cgctctggct cgtgaccgtg ctgcacgccg gcgcgctggg agacaccgtg
      901 accgagatct tcaccggacc gggcgtcgcc ggacaggtga acgtcggcgt ctcgctctgc
      961 gggttgtcga gcgtcccggc cgtcgcggtc cagctgtacg agaccggcag cgtgccggcc
     1021 gagccggtcg ccgacagcgc gatcggcgcg ttgaccgacg tctccagcgg cgatgcctcg
     1081 agcgcggtga gcgtggcgca gatctgcgtc gtgccggtca cgacgaccgt gccctgcccg
     1141 ggagccatcg ccgagcagtt gagctggagg ccgaccggcg tgatcgcgaa cgccgagatc
     1201 gagaacccgg ccgagcccga gcagctgaac gcgccgtcga ccgatgccgc cgacagcgcg
     1261 atcgtgtagc cgctcgccgc cgggagcgcg ccggcctgga acgcgatcac gttgctgaac
     1321 cggaccgcga cgctgccggt ccggctgaag ccggccgccg cgttgctgat cgtccagctc
     1381 accgtatcga tcgtgacggc gggcgcgatg gccagatcca tggtcatgct gcccgccggc
     1441 ggatcgggct cggacgagca gccgatcgtc aggctcagga tcgccgccac cacgcccacc
     1501 acgaccccca ccaccatcgc tcgcacgcac ctcatgacag ctcctttgtt cggccttgtt
     1561 cggtccagag gcgcagtcta cggacgagcg catcgcgaac aagaacaacc acgagccgct
     1621 gcggggcaga atctggaacc gcttgtccac gctggaactc cagtacggga cacagccttt
     1681 ccaacacggg atctcgaccg tgcgtcgacg tcacgtggcg tcagcgcacc ttcgcgcctc
     1741 gcagcggtgc gcgccgcagc acgttcaccg acacgcggcc cacagccgcg tcgcgccg
//
LOCUS       DASVOR010000314         2488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_178338,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000314 DASVOR010000000
VERSION     DASVOR010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178338"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..828)
                     /locus_tag="VF516_06540"
     CDS             complement(100..828)
                     /locus_tag="VF516_06540"
                     /inference="COORDINATES: protein motif:HMM:NF025559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-amino-thiazoline-4-carboxylic acid
                     hydrolase"
                     /protein_id="HEX8107369.1"
                     /translation="MAVCYRRGSTAGQTMRQLHPYYEAHRPAMEATMRQRLDLIDDLL
                     RERLGLADAAALKQDIMAEFEIVLTQMPYVGGAESRMSDFFMRLLGFMAIGRVLKRRG
                     MSDDLIQEIELESFKRHLLTIPEAERMEAGRQFMSDDNRELIRRQAEQSHQKVYPGDF
                     VYDFVDPVPGDTFEFGVNYRSCGFCQFAVRHGDRDILPNICGLDFAAYELRGIHLERT
                     QTLAGGGPHCDFRFSRKKAAAPRE"
     gene            1509..1892
                     /locus_tag="VF516_06545"
     CDS             1509..1892
                     /locus_tag="VF516_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107370.1"
                     /translation="MEAVTLPDGVIFHQIALTWDRRHPEAEGICYLDPNLCGLDEFGD
                     PSFCTLIAIMERDMKLAPYAEKPGYHAYTMLWRAPGSGAEYQEVPLRVVTIAQRGEDL
                     RVRLLVLKDDGSIARVIALHEEQPT"
     gene            complement(1951..>2488)
                     /locus_tag="VF516_06550"
     CDS             complement(1951..>2488)
                     /locus_tag="VF516_06550"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8107371.1"
                     /translation="MAAVLRGRRVAALVWIAAIGAAAGVALLSFDGGAPNAGDAWCAL
                     CAVIWAIYITRLEHFAPQFPARTLTAVHVCAVVPWTALVAAMDGALTAPVASFPWPHV
                     LYLGIVGSAATAWLQVIGQAEVPAPTAAIIYTLEPVWASLLAMIVFGDSFGWRGWLGA
                     ALILCAAVASTRAPPPDS"
BASE COUNT          436 a          847 c          833 g          372 t
ORIGIN      
        1 cgagctcggc gatcgcggcg gggcggcgag ctggctggcg gcggcgtcgg cggagcgcgg
       61 cgagctgccg atcgaggacg atcagccgag gggctgcact cactcgcgcg gcgcggccgc
      121 cttcttccgg ctgaaccgga aatcgcaatg cgggccgccg ccagcgagcg tctgcgttcg
      181 ctccagatgg atgccgcgca actcataggc cgcgaaatcg agtccgcaga tgttcggcaa
      241 gatgtcgcgg tcgccgtggc gcaccgcgaa ctggcagaac ccgcacgaac gataattcac
      301 gccgaactca aacgtgtcgc ccggcaccgg atcgacaaag tcatagacga aatcacccgg
      361 ataaaccttc tggtgactct gctcggcctg gcggcggatg agttcccgat tgtcgtccga
      421 catgaactgc cgccccgctt ccatccgctc cgcctccggg atggtgagga gatgccgctt
      481 gaagctctcc agctcgatct cctggatcag atcatcgctc atgccgcgcc gcttcagcac
      541 acgcccgatc gccatgaagc cgagcagccg catgaagaag tcggacatcc ggctctcggc
      601 tccgccgacg taaggcatct gcgtcaggac aatctcgaac tcagccatga tgtcctgctt
      661 gagcgcggca gcgtccgcca gccccaagcg ctcgcgcagc aggtcgtcga tgaggtccag
      721 gcgctggcgc atggtcgcct ccatcgccgg ccgatgggcc tcgtagtaag gatgaagctg
      781 cctcatggtc tgccccgcgg tcgagccgcg acgatagcaa accgccacga tagcgtgcgg
      841 cgctatccgc ggcgtcggtc gatttgctct caccagcgcc gcgcccgcgt cacgggctcg
      901 gcctcgccga ccggatcgat atccgcccag tcggtcgcca gcacggcctg ccagcacgtg
      961 ttttgacgtg cgtactcgat gtggttttct tcggcaaatc gacgaccatc cgacagggcc
     1021 gccggcgatg ccgtgaatgc tgaccggatc gttgctgaca gtgccacccg gacccagccg
     1081 agccagagcc gaggaaaaca agcgccgttt cgccggtggt gggcggagac gacctacggg
     1141 agtccgcagc cgcggagcga cgcggcgtgg ggtggagggg acgcttgccg cgcaggactg
     1201 gcgaggatct cctacgtccg cgcaaattgc cgtactccat tcctcggacg gatacatgtc
     1261 gtgtacgagc tgttgccacc gacccgccct gcgtgaacat gctccaccgc accacggcag
     1321 gctgtgccgt cttcacgagt gacacgcacg ccccccttgc ggagtcgatt cccgcaactc
     1381 attcacattc tagaaagagg ggagcgaggc gacgcgaatg aaccatggca catcgcctgc
     1441 agtcctcagg aaacagagga ctacacgcca tgagccaacg agccgtccga tatgcatccg
     1501 attatctcgt ggaggcggtg acgcttcccg atggagtcat cttccaccag atcgcgctga
     1561 cctgggatcg acgccatccc gaggcggaag gcatctgcta cctcgatccg aacctctgcg
     1621 ggctggacga gttcggggat ccgagctttt gcacgttgat cgcaatcatg gagcgcgaca
     1681 tgaagctcgc gccgtacgcc gagaagccgg gatatcacgc gtacacgatg ctgtggcgcg
     1741 cgcccggcag cggcgccgag taccaggagg tgccgctgcg cgtggtcacg atcgcccagc
     1801 gcggcgagga tctgcgggtc cgcctgctgg tcttgaagga cgatggctcg atcgcgcgcg
     1861 tcatcgcgct gcacgaggag caaccgacct gatcacggcc ggggcgcggg cggctttgtg
     1921 tggtggtaat ggcggtcggg tagagggtgt ctagctatcc ggcggcgggg cccgggtcga
     1981 cgccaccgcg gcgcacagga tcagcgcggc gccgagccag ccgcgccagc cgaacgagtc
     2041 gccgaacacg atcatcgcga gcaggctcgc ccacaccggc tcgagcgtgt agatgatcgc
     2101 ggcggtcggg gccggcacct cggcttgccc gatcacctgc agccacgcgg tcgccgccga
     2161 gccgacgatg ccgaggtaca gcacgtgcgg ccacgggaag ctcgcgaccg gcgcggtcag
     2221 cgcgccgtcc atcgccgcga ccagcgccgt ccacggcacg accgcgcaga cgtggacggc
     2281 ggtgagcgtg cgcgccggga actgcggcgc gaagtgctcc agccgcgtga tgtagatcgc
     2341 ccagatcacg gcgcacagcg cgcaccacgc gtcgccggcg ttgggcgcgc cgccgtcgaa
     2401 gctgagcagg gcgacgccgg ccgcggcgcc gatcgcggcg atccacacca gcgcggcgac
     2461 ccggcgcccg cggagcaccg cggccatc
//
LOCUS       DASVOR010000315         6599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_236603,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000315 DASVOR010000000
VERSION     DASVOR010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236603"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF516_06555"
     CDS             <1..292
                     /locus_tag="VF516_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107372.1"
                     /translation="EPTAALGMAQTHEVLELIKRLRAQGLGVVLISHNLANVYEVADR
                     ITVLRLGRYVGTYDAKATSREQLIAFMTGAAPGGEAPAVSEGAIGHREAPAP"
     gene            289..1512
                     /locus_tag="VF516_06560"
     CDS             289..1512
                     /locus_tag="VF516_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107373.1"
                     /translation="MSSTLERDPRVIAESTGVRGVLAEYGRRLRSGDIGQLPVMVSLV
                     VIAAIFQVLSDGRFLTPFNVVNLTLQMAAGGTISIGLVLVLLLGEIDLSAGVVSGMCA
                     AIMAVRSVEAGLPGPAAVGIALAAGAVIGAVQGGWATQFGIPSFIVTLAGLSAWGGGV
                     LLVLGRTGTVNVRDPFITALAGTFLTGPAAYGCAALFVLYVALGPLVTHRRRSAAGLT
                     VPPLAFAVIRAVAIAAFALVAVVVLAADRGISLSLVFLLAAVIVMDFVLRRVRFGRMI
                     YATGGNPEAARRAGIPVQRVRIAVFALCSMFAAWGGILGASRLLAVNQSAGQGPVLLN
                     AIAAAVIGGVSLFGGRGSMWAALLGTMVIQSISNGMDLLSLSSGIKFMVTGGVLLITV
                     TIDSVVRRSRAAAAR"
     gene            complement(1694..2224)
                     /locus_tag="VF516_06565"
     CDS             complement(1694..2224)
                     /locus_tag="VF516_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107374.1"
                     /translation="MIDLQRKVNRTVQGFVAQVIELVRHTALETLQTAFTDSAATSPA
                     AARSDRGPAAGSPGRRRTPDDLDALARRLAMVVRTHPGLRIAELAERLGTRRRALSVP
                     IRKLIAEGVIEARGRRRATTYVAAAAPEAPDAEPKAAAPVPVGPAPPVAPSAGFDADW
                     YRCLTGVYMLAHKLQK"
     gene            complement(2264..2773)
                     /locus_tag="VF516_06570"
     CDS             complement(2264..2773)
                     /locus_tag="VF516_06570"
                     /inference="COORDINATES: protein motif:HMM:NF019228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107375.1"
                     /translation="MAMPVTLIRAKFLALKPVMDERMTRLWAGAEAEAIGAGGITLVA
                     AATGMSRTTIRAGRDELLVVAETGGSGALARRWKAELQRFADRAGLTIAVSYFPPATS
                     KWTRVEHRVIHRVTESECGRPVIHRETAVQLIAGPRPAAGPGALPPLDARGLPAEWNY
                     TLHPRRDRW"
     gene            3268..4608
                     /locus_tag="VF516_06575"
     CDS             3268..4608
                     /locus_tag="VF516_06575"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8107376.1"
                     /translation="MKPSRHLATVSLILPASLLAACSPSSDATASDPAAQDPGVAVAT
                     QEDDPAAGPHVNAVTEALTGFDGLPNGNCTADVFREAGLTFREVEAFEDGIGPVFNNT
                     SCVSCHESPRGTAEAQEDNTNTKVFGGTASQITELRAGHFNGLTFVNHPGGSLINDRA
                     NDRSIQEHILSGNEVQTLRLTISLAGDGYVEAINSNTLLAIANAQPAAQRGTLIQVPV
                     LEAAGQNRTGRFGWKNQHSSLLSFASDAYINEMGVTNRFAQTENTSNGTVVQGGAFDG
                     KADPAGTGEDDAGDIDAFTTFMRCLKAPPRGPQNSSTANGQIQFNNAGCAVCHVGTIT
                     TAAAGTVINGGALTVSTALGDKNIHPFSDFLLHDVGTGDGIVQNGGQGTRNQVRTAAL
                     WGIGARTRFMHDGASLTVTAAIQRHGNQAAAAKNAFNALSATNQTDLVNFIFSL"
     gene            complement(4681..>6599)
                     /locus_tag="VF516_06580"
     CDS             complement(4681..>6599)
                     /locus_tag="VF516_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HEX8107377.1"
                     /translation="CHHTWRTRAGLAACGLVAAAGCAAGHEILPGHGPPDAVALDAPR
                     TAFVQLFEWRWTDIARECETFLGPKGFAAVQISPPGEHAWYTTGDGAPYPWWMRYQPV
                     SYSLDSSRSGSRAQFIDMVNRCNAVGVGIYVDAVFNHMTSGSGSTSSAGNDPWGVESY
                     PRVPYGPGDFHPTCTVNNDQDASNVQTCQLVGLQDLNTGSSYVQGKIEDYLTDLLHIG
                     VKGFRVDAAKHISPGDLGPIVNAVNARAASQPYWFFEVIGAAGEAVQPSQYFGLDGNL
                     ASVTEFAYGQQIFGKFAGGGKLADLRTFGESWGLMPSNRGVAFIDNHDTQRGHAGGGS
                     VLTYHNGPTYTNAVVFMLAWPYGYPVIMSSYAFNTSSAFDTSYGPPFDANTGATRGPW
                     DGGVSQPACFNQSIGGWVCEHRFRPIGNMVGFRNATVGNWFVSDWWDDGNNQIAFGRG
                     NQGFVVINQEGNALTRSFHTSLPAGNYCEIISGDFTPGTGGGAPTCSGTVVAVDGSGN
                     VTLTAPAFGGAAIHVGAMIPNGPTPPPPTTVTVTFNEAADTFFGQNIFVTGSINELSN
                     WSTSNPVPLAWISGQGTRGNWRATVTLPASTHVEYKYIKKDGSNNVIWESGANRVLQT
                     GAGGTSQTTSDAWK"
BASE COUNT          957 a         2249 c         2368 g         1025 t
ORIGIN      
        1 cgagccgacc gccgcgctcg gcatggccca gacccacgag gtgctcgagc tgatcaagcg
       61 gctgcgcgcc cagggcctgg gcgtggtgct gatcagccac aacctcgcca acgtgtacga
      121 ggtcgccgac cggatcaccg tgctgcggct cgggcgctac gtcgggacct acgacgccaa
      181 ggcgaccagc cgcgagcagc tgatcgcgtt catgaccggc gcggcgccgg gcggcgaggc
      241 cccggcggtg tccgaggggg cgatcggcca ccgcgaggcg ccggcgccat gagcagcacg
      301 ctggagcgcg atccgcgggt gatcgccgag tccaccgggg tgcgcggcgt gctggccgag
      361 tacggccggc ggctgcggtc cggcgacatc gggcagctgc cggtgatggt gagcctggtc
      421 gtcatcgcgg cgatcttcca ggtgctgtcc gacggccggt tcctcacgcc gttcaacgtg
      481 gtcaacctga ccctgcagat ggcggccggc gggacgatct cgatcggcct ggtgctggtc
      541 ctgctgctcg gcgagatcga tctgtccgcc ggcgtggtca gcgggatgtg cgcggcgatc
      601 atggccgtgc gctcggtgga agcgggtctg cccgggccgg cggcggtcgg catcgcgctg
      661 gcggccgggg cggtgatcgg ggcggtgcag ggcggctggg ccacccagtt cggcatcccg
      721 tcgttcatcg tcacgctcgc cgggctcagc gcgtggggcg gcggcgtgct gctggtgctc
      781 ggtcggaccg ggacggtcaa cgtccgcgat ccgttcatca ccgcgctcgc cgggacgttc
      841 ctgaccggcc cggcggcgta cggctgcgcc gcgctgttcg tgctctacgt ggcgctcggt
      901 ccgctggtga cccaccgccg ccgcagcgcc gccgggctca ccgtgccgcc gctcgcgttc
      961 gcggtgatcc gagccgtcgc gatcgccgcg ttcgcgctcg tggcggtcgt cgtgctcgcc
     1021 gccgatcgcg ggatcagctt gagcctggtg ttcctgctcg ccgccgtgat cgtgatggac
     1081 ttcgtgctgc gccgggtccg gttcggccgg atgatctacg cgaccggcgg taaccccgag
     1141 gcggcgcggc gcgccggcat cccggtccag cgggtgagga tcgcggtgtt cgcgctgtgc
     1201 tcgatgttcg cggcgtgggg cggcatcctc ggcgcgtcgc ggctgctcgc ggtcaaccag
     1261 tcggccgggc agggcccggt gctgctcaac gcgatcgcgg ccgccgtgat cggcggcgtc
     1321 agcctgttcg gcggccgcgg ctcgatgtgg gccgcgctgc tcggcacgat ggtgatccag
     1381 tcgatctcca acggcatgga cctcttgtcg ctgagctcgg ggatcaagtt catggtcacc
     1441 ggcggtgttc tgctgatcac cgtcaccatc gactcggtgg tccgccgcag ccgcgccgcg
     1501 gccgcgcggt agctctgcgc gtggggccgc gaatcggcgg cgcagcgctg gactccgcat
     1561 gtccgtgatc atgcggaatc atgacattcg atctgcgatc acacgcgcgg tgcagcgcgg
     1621 tgcgtgatgg cagcgcacga tgacgatcgg agcgcatgcc gtgctcgcgc gcagccatcg
     1681 cggtggcgac gcgtcacttc tggagcttgt gggcgagcat gtagacgccg gtgaggcagc
     1741 ggtaccagtc ggcgtcgaac cctgccgaag gggcgaccgg cggagccggg ccgaccggga
     1801 ccggcgcagc ggctttaggc tcggcatccg gcgcttccgg cgcggccgcg gcgacgtacg
     1861 tggtcgcgcg ccggcggccc cgggcctcga tcacgccctc ggcgatcagc ttgcggatcg
     1921 gcaccgagag ggcgcggcgc cgggtaccga gccgctcggc gagctctgcg atccgcagcc
     1981 cggggtgggt ccggacgacc atcgcgagtc gcctcgccag ggcatcgagg tcgtccggcg
     2041 tgcgccgccg gccggggcta cccgcggcgg gcccgcgatc gctgcgcgcg gccgccgggg
     2101 aggtcgccgc ggagtcggtg aacgcggtct gcagggtctc gagcgcggtg tggcgcacga
     2161 gctcgatgac ctgcgccacg aagccttgca cggtgcgatt taccttgcgc tggaggtcga
     2221 tcatggatga ctcatcggtc ctcttcgctg ccgggccgga cgatcaccag cggtcacggc
     2281 gaggatgaag ggtgtagttc cactcggcgg gaagcccgcg cgcgtcgagc gggggcaggg
     2341 cgccaggacc cgccgccgga cggggcccgg cgatcaactg caccgcggtc tcgcgatgga
     2401 tcacggggcg gccgcactcg ctctcggtga cgcgatggat cacgcggtgc tcgacccgcg
     2461 tccacttcga ggtggcgggc gggaagtacg acaccgcgat cgtcaggccg gcgcggtcgg
     2521 cgaaccgctg cagctcggcc ttccaccggc gggcgagcgc gccgctaccg ccggtctcgg
     2581 cgaccacgag cagctcgtcc cgtccggccc ggatcgtggt tcgcgacatc ccggtcgccg
     2641 ccgcgaccag cgtgatcccg ccggcgccga tcgcctcggc ctcggcgccc gcccacagcc
     2701 gggtcatgcg ttcatccatc accggcttca gggcgaggaa cttcgctcgg atcagcgtga
     2761 cgggcattgc catggacagg taattctcga gcagggactg gcccgctgcg atgccgggag
     2821 tgcataccac gagcaccggt ggacaggtta atcttgagca gtgatagacg atcggtttta
     2881 cgtattatgc gtctcacgat cgaattgcgc tgatgactcg tcggtggccc gccagccgaa
     2941 aaactgaggc gattccccct cggggcagaa cgcccccttc ggaaaactga aagtctggcc
     3001 tgtgtaatat gggagtgagg ggtggtggtc tcacaaacga ctcctcctga gaactctgta
     3061 ttagtgagat cgcgattgac gtggagttgt gcgatcgaca gagtgctgca cgtcaagaca
     3121 cggccaagca cgaacgcggt gccgacatgt gcttcggaat caccttgcaa ggaggacgac
     3181 cgcggtccgt cgacgcgctg cgcccgtcgt tgttcacggg tgcccaccac ccgtcattgc
     3241 attgccatcc tgaggagaat cacagtcatg aaaccatctc gccatctcgc taccgtctcg
     3301 ttgatcctgc cggcgtcact gctcgcggcg tgctcgccgt cgagcgacgc cacggcctcg
     3361 gatcccgcgg cgcaggatcc cggcgtcgcg gtcgcgaccc aggaggacga tcccgccgcc
     3421 ggcccgcacg tcaacgcggt caccgaggcg ctgaccggct tcgacggcct gcccaacggc
     3481 aactgcaccg cggacgtttt cagagaggcg ggcctcacgt tcagggaggt cgaggccttc
     3541 gaggacggca tcggcccggt gttcaacaac acctcgtgcg tcagctgcca cgagagcccg
     3601 cgcggaaccg cggaggcgca ggaggacaac accaacacaa aggtcttcgg cggcaccgcc
     3661 agccagatca ccgagctccg cgccggtcac ttcaacggcc tcaccttcgt caaccacccg
     3721 ggcggctcgc tgatcaacga tcgcgcgaac gaccgctcga tccaggagca catcctgtcg
     3781 ggcaacgagg tccagacgct ccggctcacg atcagcctgg cgggtgacgg ctacgtcgag
     3841 gcgatcaaca gcaacacgct gctggcgatc gccaacgccc agccggccgc gcagcgcggc
     3901 acgctgatcc aggtcccggt gctggaggcc gcgggccaga accgcaccgg ccggttcggc
     3961 tggaagaacc agcactccag cctgctgtcg ttcgcctcgg acgcctacat caacgagatg
     4021 ggcgtcacca acaggttcgc gcagaccgag aacacgtcca acggcaccgt ggtgcagggc
     4081 ggcgcgttcg acggcaaggc cgatccggcc ggcacaggcg aggatgacgc gggcgacatc
     4141 gacgcgttca ccacgttcat gcgctgcctg aaggcgccgc cgcgcggccc gcagaacagc
     4201 agcaccgcca acggccagat ccagttcaac aacgccggct gcgcggtctg ccacgtcggc
     4261 accatcacca ctgccgcggc gggcaccgtg atcaacggcg gcgcgctcac cgtgtcgacc
     4321 gcgctcggcg acaagaacat ccatccgttc agcgacttcc tgctccacga tgtcggcacc
     4381 ggcgacggca tcgtgcagaa cggcggtcag ggcacgcgca accaggtccg caccgcggcg
     4441 ctgtggggca tcggggcgcg cacccggttc atgcacgacg gcgcgagcct gacggtgacg
     4501 gcggcgatcc agcgccacgg caaccaggcc gcggccgcga agaacgcctt caacgccctc
     4561 agcgcgacga accagaccga cctggtgaac ttcatcttct cgctgtaagc gggcatccga
     4621 ggcgacagac cgcggccacc tgtcgcgggc gcccggctgc gagggcgggc atccgcgcca
     4681 ctacttccag gcgtcgctgg tggtctggct ggtcccgccg gcgccggtct gcagcacgcg
     4741 gttggcgccg gactcccaga tcacgttgtt ggagccgtcc ttcttgatgt acttgtactc
     4801 gacgtgggtc gacgccggca gggtcaccgt ggcccgccag ttgccgcgcg tgccctgtcc
     4861 cgagatccac gccagcggca ccggattgct ggtgctccag ttcgacagct cgttgatgct
     4921 gccggtcacg aagatgttct gaccgaagaa ggtgtcagcg gcctcgttga acgtcaccgt
     4981 cacggtggtg ggcggcggcg gcgtgggccc gttcgggatc atggcaccca cgtggatcgc
     5041 cgcgccgccg aaggcaggtg ccgtgagcgt gacgttgccg gagccatcga ccgcgaccac
     5101 cgtgcccgag caggtcggcg cgccgccgcc tgtgccgggc gtgaagtccc ccgagatgat
     5161 ctcgcagtag ttcccggccg gcaggctggt gtggaagctg cgggtcagcg cgttgccctc
     5221 ctggttgatc acgacgaagc cctggttgcc gcggccgaac gcgatctggt tgttgccgtc
     5281 gtcccaccag tcggacacga accagttgcc caccgtcgcg ttgcggaacc ccaccatgtt
     5341 gccgatcggg cggaaccggt gctcgcagac ccagccgccg atcgactggt tgaagcacgc
     5401 cggctggctg accccgccgt cccacgggcc gcgggtcgcg ccggtgttcg cgtcgaacgg
     5461 cgggccgtag ctggtgtcga acgcgctgct ggtgttgaac gcgtagctcg acatgatgac
     5521 cgggtagccg tacggccacg ccagcatgaa cacgaccgcg ttggtgtagg tcgggccgtt
     5581 gtggtaggtc agcacgctgc cgccgccggc gtggccgcgc tgggtgtcgt ggttgtcgat
     5641 gaacgcgacg ccgcggttgc tcggcatcag gccccacgac tcgccgaacg tgcgcagatc
     5701 ggcgagcttg ccgccgccgg cgaacttgcc gaagatctgc tggccgtagg cgaactcggt
     5761 gacgctggcg aggttaccgt cgaggccgaa gtactggctg ggctggaccg cctcgccggc
     5821 ggcgccgatc acctcgaaga accagtacgg ctggctcgcc gcccgcgcgt tgaccgcgtt
     5881 gacgatcggc ccgaggtcgc cggggctgat gtgcttggcg gcgtcgaccc ggaagccctt
     5941 gacgccgatg tggagcaggt cggtcaggta gtcctcgatc ttgccctgga cgtaggacga
     6001 gccggtgttg aggtcctgca ggccgaccag ctggcaggtc tggacgttac tggcgtcctg
     6061 gtcgttgttg acggtgcacg tcgggtggaa gtcgccgggg ccgtagggca cgcgcgggta
     6121 gctctcgacg ccccacggat cattgccggc gctcgaggtg gagcccgagc cgctggtcat
     6181 gtggttgaac accgcgtcga cgtagatccc gacgccgacc gcgttgcagc ggttcaccat
     6241 gtcgatgaac tgggcgcggc tgccgctccg gctggagtcg aggctgtagc tgaccggctg
     6301 gtagcgcatc caccacgggt acggcgcacc gtcgccggtg gtgtaccagg cgtgctcgcc
     6361 gggcggcgag atctggaccg cggcgaaccc cttgggcccg aggaaggtct cgcactcgcg
     6421 cgcgatgtcg gtccacctcc attcgaacag ctggacgaac gcggtgcgcg gggcgtcgag
     6481 cgcgaccgcg tcgggcggtc catggccggg caggatctcg tggccggccg cacagccggc
     6541 ggcggcgacg aggccgcacg ccgcaagccc tgcgcgcgtc ctccacgtgt ggtggcagg
//
LOCUS       DASVOR010000316         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_295206,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000316 DASVOR010000000
VERSION     DASVOR010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295206"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="VF516_06585"
     CDS             <1..822
                     /locus_tag="VF516_06585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107378.1"
                     /translation="RDSARTARTGGDGRFEIADAVDDAVVIAELGDRRAAPAVAGDNL
                     RLTLAPTSRLEGKVDLAGEVPDNVVIVVRDVEWPVAPRYSIRAPVAADGSFSLDGVPR
                     GQVRVYAAIDGLIGRTTSSATVTVLAPVVRGVALSVARSSRVVHVLVRNTVNTRLANA
                     EVVVLPGRVPSTSLLEMNRQFRGGSVRWARQLEGEHAPKEIVAAAQPGDLFATMTEVP
                     DGTASACALGLPELSDEELEHKLYVHLDRIQVICTPIPDGASFVMIQVPPFPRLD"
     gene            complement(838..1779)
                     /locus_tag="VF516_06590"
     CDS             complement(838..1779)
                     /locus_tag="VF516_06590"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107379.1"
                     /translation="MAKPLEYNATLTERVDITDALSLFRITPDQPPAQRPWFVPGQYC
                     VLGLNNTEKPELGSVRRSMSIASAPEDGGPIEFYIRWVAKPESDNPLTHLLWKARSGD
                     RMYMRAVAAGVFTVPDTVGVDDPRLRIMVAAGTGSAPFLSMVRSELRRNPSADLSRWV
                     LLHGASYPADLGYRDELLQMVERNHLRYWGTVSRPKEAPDWKGDTGRVEGFFEGARLD
                     DLERRLGLPPGGFTPKSAVILICGLTGTITGTILPLLDRGFVPEVKRIREALGVPAER
                     HASVFWEQYDTEPIINIKDPNVVEPLRARMQAALARL"
     gene            complement(1895..>2081)
                     /locus_tag="VF516_06595"
     CDS             complement(1895..>2081)
                     /locus_tag="VF516_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107380.1"
                     /translation="ALAEASGVDLAKVGYTPELLAQAFGDKLDVTQIGELRWDKIEDR
                     LMGLVPHEFKALICTRP"
BASE COUNT          276 a          744 c          755 g          306 t
ORIGIN      
        1 cgcgactcgg cgcgcaccgc gcggaccggc ggcgacggcc ggttcgagat cgccgacgcg
       61 gtcgatgacg ccgtggtgat cgccgagctc ggcgatcgcc gcgccgcgcc ggccgtcgcc
      121 ggcgacaacc tccggctcac gctcgcgccg accagccggc tcgaaggcaa ggtcgatctc
      181 gccggcgagg tcccggacaa cgtcgtgatc gtcgtgcgcg acgtcgagtg gccggtcgcg
      241 ccgcgttatt cgatccgcgc gccggtcgcc gccgatggct cgttcagcct cgacggcgtg
      301 ccgcgcggcc aggtccgggt gtacgccgcg atcgacggcc tgatcgggcg gacgaccagc
      361 agcgcgacgg tcaccgtgct cgccccggtg gtgcgcggcg tcgcgctgtc ggtcgcgcgg
      421 tcgagccgcg tcgtccacgt cctggtccgc aacaccgtca acacccggct cgccaacgcc
      481 gaggtcgtgg tcctgccggg ccgggtgccg tcgaccagcc tgctcgagat gaaccgccag
      541 ttccgcggcg gcagcgtgcg ctgggcgcgc cagctcgaag gcgagcacgc gccgaaggag
      601 atcgtggcgg ccgcgcagcc gggcgatctg ttcgcgacga tgaccgaggt gcccgacggc
      661 acggccagcg cctgtgcgct cggcctgccc gagctgtccg acgaggagct cgagcacaag
      721 ctgtacgtcc acctcgaccg gatccaggtg atctgcacgc cgatccccga cggcgccagc
      781 ttcgtgatga tccaggtgcc gccgttcccg cgcctcgact agcctgctat gtcccggtca
      841 cagccgggcg agcgcggcct gcatgcgggc gcgcagcggc tcgacgacgt tcggatcctt
      901 gatgttgatg atcggctcgg tgtcgtactg ctcccagaac accgaggcgt gccgctcggc
      961 cgggaccccg agcgcctcgc ggatccgctt gacctccggg acgaagccgc ggtcgagcag
     1021 cgggaggatc gtgccggtga tcgtgccggt caggccgcag atcaggatca cggcgctctt
     1081 cggggtgaag ccgccgggcg gcaggccgag ccggcgctcg aggtcgtcga gccgcgcgcc
     1141 ctcgaagaac ccctcgacgc ggccggtgtc gcccttccag tccggcgcct ccttgggccg
     1201 gctgaccgtg ccccagtacc gcagatggtt gcgctcgacc atctggagca gctcgtcgcg
     1261 gtagccgagg tcggcggggt aggacgcgcc gtggagcagc acccagcgcg acaggtcggc
     1321 gctcgggttg cggcgcagct cgctgcgcac catgctgagg aacggcgccg agccggtgcc
     1381 ggcggcgacc atgatgcgca gccgcggatc gtcgacgccg accgtgtccg ggaccgtgaa
     1441 cacgccggcc gcgaccgcgc gcatgtacat ccggtcgccg gacctggcct tccacaggag
     1501 gtgggtcagc gggttgtcgg actcgggctt ggcgacccac cggatgtaga actcgatcgg
     1561 cccgccgtcc tcgggcgccg acgcgatcga catcgagcgc cggaccgagc cgagctcggg
     1621 cttctcggtg ttgttgaggc cgagcacgca gtactgtccg gggacgaacc acggccgctg
     1681 cgccggcggc tgatcgggcg tgatgcggaa caggctgagc gcgtcggtga tgtcgacccg
     1741 ctcggtcagc gtcgcgttgt actccagggg cttcgccatc ggcgcgacgc tatcacggcg
     1801 gtcgatcgat ggggcgttcc catgaccccc ctttggggga tgccgcggca aagccgcggc
     1861 attcacccct ccgcgcgtcg cgtcgcgcgg tcttctacgg ccgcgtgcag atcagcgcct
     1921 tgaactcgtg cggcaccagc cccatgagcc gatcttcgat cttgtcccag cgcagctcgc
     1981 cgatctgcgt gacgtcgagc ttgtcgccga acgcctgggc caggagctcg ggcgtgtagc
     2041 cgaccttggc gaggtcgacg ccgctggcct cggcgagcgc g
//
LOCUS       DASVOR010000317         3399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_3011,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000317 DASVOR010000000
VERSION     DASVOR010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3011"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="VF516_06600"
     CDS             complement(<1..854)
                     /locus_tag="VF516_06600"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX8107381.1"
                     /translation="MTFLSIDRPMTQAQRASKIRGVQLAIVVDNKDGGDNPGYRVKVK
                     FPWLNDQETTFWARIAVPMSGNDRGTYVLPEIDDQLLVVFEHGDVDRPIVIGALWSKK
                     QEPVEVNDSGKNNTKLIKSRCGHRIIFDDKQGAEKITIVDSTKKNKVILDSVNKIVKL
                     ECDGDIEIKAKTNVIMHSNALKVGTKEGITGKGQSLLTHSQKTFGVKASSGITIGGGN
                     TTINVSNSAATSVSGSGAGELGGAAEEQPKDQVQAQQAGGGGGGGGGGGGGGGGGGGG
                     GGGGSSTSA"
     gene            complement(858..1547)
                     /locus_tag="VF516_06605"
     CDS             complement(858..1547)
                     /locus_tag="VF516_06605"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX8107382.1"
                     /translation="MDAVQYGLHYAIVCQNQDPDKLDRIKVRLPWLDAGDTDQTHWAQ
                     LLTPMEGNKFGWYVLPDVDDVVVVMFIGGDISQPVIVGGVWSTPDFSPEPNEDGANNF
                     RGYRSRVGHRMVFDDTKSGTKVWFADKTTKLMVGMGKFAKDGAGPNICAVWKPPMSGE
                     NGVSISSMEGKMEITAKTKLTIKGENIKINAKTTIDIKAGTDFQMEGSSSAKLTSSSE
                     SNYDAPKIDIE"
     gene            complement(1578..2636)
                     /locus_tag="VF516_06610"
     CDS             complement(1578..2636)
                     /locus_tag="VF516_06610"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEX8107383.1"
                     /translation="MGDSSAASPNVDILIDGAEASDAHFQSYIVDRDMYQPDMAAIVL
                     ANQGDVYSSKKVGASIEIKVGNDKTSIFKGEIVGLEPVYKGGDKTIITIRAMNKLHRL
                     LRKRKSITFTDKSDQQILNQVVGDAGLTLDWKHETSITYKHVYQHNQSDLEFLRTRAA
                     RMGCHVWCVDTTVNVKQPDLQSGPIATLNVDQEADAILHRLAPRLNSSAVINKVTVKG
                     WNPETKELITGDATVQGSKLGSQTSVAGSGSLGNEETFTVDHPIWSAEEAKALAKARL
                     QDASLSYITGEAEVAGNPVFDLGKVVKVVANAMKGDDPFNGNYYIMGLTHRHIAAKAK
                     DGGGYVTILRFARDAQAQ"
     gene            complement(2640..3173)
                     /locus_tag="VF516_06615"
     CDS             complement(2640..3173)
                     /locus_tag="VF516_06615"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107384.1"
                     /translation="MSNFLHPSSKSGVVSIASLDTSIQVDAQYNPKELQVDKTVPWQK
                     KSQANKTQETGIQLEFTGAEGRSLTLELLFDGFETGSSVAGKVETLNTMASVLEPGSP
                     DETKRRPHLCVVTWGTTVPTFKCVIESLSVKYTMFSDQGVPLRATCTVKLKEADTVTG
                     QAEGGSGGSGGGSGGGT"
     gene            complement(3170..>3399)
                     /locus_tag="VF516_06620"
     CDS             complement(3170..>3399)
                     /locus_tag="VF516_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107385.1"
                     /translation="FAELTLVTAAPSSQIAASTRSAPSAVSPTPGPGAAAQQSGSPAP
                     QIDIEKLANEVYRNILVMMDVARARNGDPYL"
BASE COUNT          457 a         1123 c         1116 g          703 t
ORIGIN      
        1 gcgctggtcg agctgccgcc gccgccacct ccaccaccac cgccgccgcc tccgccacca
       61 ccgccgcctc cgccaccgcc gccaccggcc tgctgcgcct ggacctgatc cttgggctgc
      121 tcctcggccg cgccgccgag ctcacccgcg cccgaaccgg acacgctggt cgccgccgag
      181 ttcgagacgt tgatggtcgt gttgccgccg ccgatcgtga tgccgctgga cgccttgacg
      241 ccgaacgtct tctgcgagtg ggtgagcagc gactggccct tgcccgtgat gccctccttc
      301 gtgcccacct tgagcgcgtt ggagtgcatg atcacgttgg tcttggcctt gatctcgatg
      361 tcgccgtcgc actcgagctt gacgatcttg ttgaccgaat cgaggatgac cttgttcttc
      421 ttggtgctat cgacgatcgt gatcttctcg gcgccctgct tgtcgtcgaa gatgatccgg
      481 tggccgcagc gcgacttgat cagcttggtg ttgttcttgc ccgaatcgtt gacctcgacc
      541 ggctcctgct tcttgctcca cagcgcgccg atcacgatcg gccggtcgac gtcgccgtgc
      601 tcgaacacga cgagcagctg atcgtcgatc tccggcagca cgtacgtgcc gcggtcgttg
      661 ccgctcatcg gcaccgcgat ccgcgcccag aacgtggtct cctggtcgtt gagccagggg
      721 aacttcacct tgacgcggta gccggggttg tcgcccccgt ccttgttgtc gacgacgatg
      781 gcgagctgga cgcctcggat cttcgaggcg cgctgcgcct gcgtcatcgg ccggtcgatc
      841 gataggaagg tcacgggtta ctcgatgtcg atcttgggag cgtcgtagtt gctctcgctg
      901 ctggacgtca gcttggccga gctgctgcct tccatctgga agtcggtgcc ggccttgatg
      961 tcgatcgtgg tcttggcgtt gatcttgatg ttctcgccct tgatcgtcag cttggtcttc
     1021 gccgtgatct ccatcttgcc ttccatcgac gagatcgaga cgccgttctc gccggacatc
     1081 ggcggcttcc acaccgcgca gatgttgggg ccggcgccgt ccttggcgaa cttgcccatg
     1141 ccgaccatga gcttggtggt cttgtcggcg aaccagacct tggtgccgct cttggtgtcg
     1201 tcgaacacca tgcggtggcc gacccggctg cggtagccgc ggaagttgtt ggcgccgtcc
     1261 tcgttgggct ccggcgagaa gtccggcgtg ctccacacgc cgccgacgat gaccggctgg
     1321 ctgatgtcgc cgccgatgaa catcaccacg acgacgtcgt cgacgtcggg cagcacgtac
     1381 cagccgaact tgttgccctc catcggcgtc agcagctgcg cccagtgggt ctggtcggtg
     1441 tcgccggcgt cgagccatgg cagccggacc ttgatgcggt cgagcttgtc ggggtcctgg
     1501 ttctggcaca cgatcgcgta gtgcagcccg tactggactg catccaacga ctcgatccgg
     1561 gggccgccgc tccgcatcta ttgcgcctgg gcgtcgcgcg cgaagcgcag gatggtgacg
     1621 tagccgccgc cgtccttggc cttggcggcg atgtgacggt gggtgagacc catgatgtag
     1681 tagttgccgt tgaacggatc gtcgcccttc atcgcgttgg cgacgacctt gacgaccttg
     1741 ccgaggtcga acaccgggtt gcccgcgacc tcggcctcgc cggtgatgta gctcagcgag
     1801 gcgtcctgca gccgcgcctt ggccagcgcc ttggcctcct cggcggacca gatcggatgg
     1861 tcgacggtga acgtctcctc gttgccgagc gagcccgagc cggcgaccga ggtctgcgag
     1921 ccgagcttgg agccctgcac cgtggcgtcg ccggtgatca gctccttggt ctcggggttc
     1981 cagcccttga ccgtgacctt gttgatcacc gccgacgagt tcagccgcgg cgcgagccgg
     2041 tgcaggatgg cgtcggcctc ctggtcgacg ttgagcgtgg cgatcggccc gctctgcaga
     2101 tcgggctgct tgacgttgac cgtggtgtcg acgcaccaca cgtggcagcc catgcgagcc
     2161 gcgcgggtgc gcaggaactc caggtcggac tggttgtgct ggtagacgtg cttgtaggtg
     2221 atcgaggtct cgtgcttcca gtcgagcgtc agcccggcat cgccgacgac ctggttcagg
     2281 atctgctgat cgctcttgtc ggtgaacgtg atcgacttgc gcttgcgcag caggcgatgg
     2341 agcttgttca tcgcccggat cgtgatgatg gtcttgtcgc cgcccttgta gaccggctcg
     2401 aggccgacga tctcgccctt gaagatcgac gtcttgtcgt tgccgacctt gatctcgatc
     2461 gacgcgccga ccttcttcga cgagtagacg tcgccctggt tggccagcac gatcgcggcc
     2521 atgtccggct ggtacatgtc gcggtcgacg atgtacgact ggaaatgcgc gtcgctggcc
     2581 tcggcgccgt cgatcaggat gtcgacgttg ggtgatgctg cggatgagtc gcccacggcc
     2641 taggtacccc cgccggagcc gccgccggaa ccgccgctgc cgccctcggc ctggcccgtg
     2701 acggtatcgg cttccttcag cttcacggtg caggtcgcgc gcagcggcac gccctggtcc
     2761 gagaacatcg tgtacttgac cgacagcgac tcgatgacgc acttgaacgt cggcaccgtg
     2821 gtgccccagg tgaccacgca caggtgcggc cgtcgcttgg tctcgtcggg gctgcccggc
     2881 tcgagcaccg aggccatcgt gttgagcgtc tccaccttgc cggccacgct ggatccggtc
     2941 tcgaagccgt cgaacagcag ctccagcgtc agcgagcgtc cctcggcgcc cgtgaactcc
     3001 agctggatcc cggtctcctg ggtcttgttc gcctggctct tcttctgcca cggaaccgtc
     3061 ttgtcgacct ggagctcctt gggattgtac tgcgcgtcga cctggatgct ggtgtccagg
     3121 ctggcgatcg agacgacgcc ggatttcgag ctcggatgca ggaagttgct cataggtagg
     3181 gatccccgtt tcgggcccgg gcgacatcca tcatgacgag gatgttccga tagacctcat
     3241 tagcgagctt ctcgatgtcg atctgcggcg cggggctgcc ggattgctgc gcggccgcgc
     3301 ccgggcccgg cgtcggcgac accgcgctgg gcgccgagcg ggtcgaggcc gcgatctggc
     3361 tggacggcgc cgcggtcacc agcgtgagct cggcgaacg
//
LOCUS       DASVOR010000318         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_528926,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000318 DASVOR010000000
VERSION     DASVOR010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528926"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VF516_06625"
     CDS             <1..895
                     /locus_tag="VF516_06625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8107386.1"
                     /translation="LNALDYARERKQGSSIKQWKDATAPRVPIIDHPDVRRMLLDMKA
                     RVEGLRALAVKLTMHIDHARAIEKTGGDKALAEYHTGQLDLLVPLIKAYGSDQAFQIC
                     ATAIQVYGGAGYLKDWPVEQYCRDSKIFSIYEGTNHIQAMDLVGRKLMQRGGANVQAF
                     GKDVGAFVAAHKQHPTLKDAIAVLAQAMEALTGTGGKFMQWFGGGRMEMVPAVANRFL
                     EMMAESTVGWLLLDAAVIAEAAAAGLPADHPDRAFYDGKKFAALYFAHNVLPGVAAKA
                     QLIAREDRTVLDIPAAAFGPV"
     gene            complement(945..1250)
                     /locus_tag="VF516_06630"
     CDS             complement(945..1250)
                     /locus_tag="VF516_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107387.1"
                     /translation="MVQGVCSCPRDNLCDNCLARSIEWLGGIAASRGYGWATSVARRK
                     PGLLARPWPALEGRAAELAAAKVIDLADDRRLFARLLEKLNAAARRRWDQLRAEPGP"
     gene            1732..2193
                     /locus_tag="VF516_06635"
     CDS             1732..2193
                     /locus_tag="VF516_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8107388.1"
                     /translation="MRPELARKGTKRAAVERGSAVELVARAASSMYGPPQGVDPADQL
                     RHRLGARIRALRDARGLTQEVIADRIEVTPKFVSQLECGQRSPSWETLVAIAHHGFQI
                     RLAALMFGIDEDIGAEIQDLNDMLAGRPTETRRDLLQAMELMLRAGAEPKL"
     gene            complement(2237..2530)
                     /locus_tag="VF516_06640"
     CDS             complement(2237..2530)
                     /locus_tag="VF516_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HEX8107389.1"
                     /translation="MNTEHDPHLYTARRVEPLSGGCLECLQLGLTWVHLRICLSCGHV
                     GCCDSSPGRHATAHFHTTGHPIIESFEPDEDWGWCYIHGEFIELPDELRVARP"
     gene            complement(2527..>2721)
                     /locus_tag="VF516_06645"
     CDS             complement(2527..>2721)
                     /locus_tag="VF516_06645"
                     /inference="COORDINATES: protein motif:HMM:NF039315.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5996 family protein"
                     /protein_id="HEX8107390.1"
                     /translation="EQDPHAKILEFCQSTYEAGANLAGWNRAEFERSETGGGDPDGSA
                     GGAGGSVPRGTERTGSGGPT"
BASE COUNT          408 a          957 c          962 g          394 t
ORIGIN      
        1 cctcaacgcg ctcgactacg cccgcgagcg caagcagggc agctcgatca agcagtggaa
       61 ggacgcgacc gcgccgcgcg tcccgatcat cgaccacccc gacgtccgcc gcatgctgct
      121 cgacatgaag gcgcgggtcg aaggcctgcg cgcgctcgcc gtcaagctga ccatgcacat
      181 cgatcacgcg cgcgcgatcg agaagaccgg cggcgacaag gcgctcgccg agtaccacac
      241 cggccagctc gacctgctgg tcccgctgat caaggcctac ggcagcgacc aggcattcca
      301 gatctgcgcc acggcgatcc aggtctacgg cggcgccggc tacctgaagg actggccggt
      361 cgagcagtac tgccgcgact ccaagatctt ctcgatctac gagggcacca accacatcca
      421 ggcgatggac ctggtcggcc gcaagctgat gcagcgcggc ggcgccaacg tccaggcgtt
      481 cggcaaggac gtcggcgcgt tcgtcgccgc gcacaagcag cacccgacgc tcaaggacgc
      541 gatcgcggtg ctcgcccagg cgatggaggc gctgaccggc accggcggca agttcatgca
      601 gtggttcggc ggtggccgga tggagatggt gccggcggtc gccaaccggt tcctggagat
      661 gatggccgag tccacggtcg gctggctgct gctcgacgcc gcggtgatcg ccgaggccgc
      721 ggcggccggg ctgcctgccg atcacccgga tcgcgcgttc tacgacggca agaagttcgc
      781 ggcgctgtac ttcgcgcaca acgtgctgcc cggcgtcgcg gccaaggcgc agctgatcgc
      841 gcgcgaggac cgcaccgtgc tcgacatccc ggcggccgcg ttcggcccgg tgtgacctcg
      901 gagcgcgccg gtccctcgca ccgctcgcgt tcgcgccccg gcggtcacgg gccgggctcc
      961 gcccgcagct gatcccaccg cctccgtgcg gccgcgttca gcttctcgag cagccgcgcg
     1021 aacagccggc ggtcgtcggc gaggtcgatg accttggcgg cggcgagctc ggcggctcgg
     1081 ccctccagcg ccggccacgg ccgggccagc agcccgggct ttcgccgcgc gacggaggtc
     1141 gcccagccat agccgcgcga tgcggcgatg ccgccgagcc actcgatcga ccgcgcgagg
     1201 cagttgtcgc agaggttgtc gcgcgggcag ctgcagacgc cctgcaccat gatgccagcg
     1261 tagggaggcg gttcttcccc gaacaccgac gggccgcgga gttgcggacg tgaattccgg
     1321 ctgtgtgttc cgcgcgcgcg gcgtcgatcg gaccgatcgc gtcgcgcgcg tcgaacgccg
     1381 tgtcacgggt ccggagcggg tttcgcacgg cagccgggat ctgcccggtg cagatctccc
     1441 aatcggaatg gatccacggc gttctgcgat cggttggaca acgtcgcgac cggccgcgac
     1501 cgcagcatcg tcatcgtgtc agtgcactgt gctaatgttc agtgacgcga gggacacctg
     1561 gaaccaacgg aaaatgctgg atattacaat atcttgccat cgatcccgtg gccgccaccg
     1621 ctccgggacg ttcgagctca tcgaatcgct cccgcgagct cggtgcacgg cctctgcgcg
     1681 gaagacaagc tcggcgcgtg gcgctgcgag cgcacggggc gtctcaggaa gttgcgtccc
     1741 gagctcgcgc gaaagggcac caagcgtgcg gcggtcgagc gcggcagcgc ggtcgagctc
     1801 gtcgcgcgcg ctgcgagctc gatgtacggg ccgccgcagg gcgtcgatcc tgcggatcag
     1861 ctccggcatc gcctcggtgc gcggatccgg gcgctccgtg acgcgcgcgg gctcacgcaa
     1921 gaggtcatcg cagaccgcat cgaggtcacg ccgaagttcg tgagccagct cgagtgcggc
     1981 cagcgctcgc cgagctggga gaccctggtg gcgatcgcgc atcatggctt ccagatccgg
     2041 ctcgccgcgc tgatgttcgg gatcgacgag gacatcgggg ccgagatcca ggatctcaac
     2101 gacatgctcg ccggccggcc gacggagacg cgccgcgacc tgctgcaggc gatggagctg
     2161 atgctccggg cgggcgccga accgaagctg tgagcgcgga ccgtcgtgga ccgtcgtgga
     2221 ccgggctgct cgcccgtcag ggccgcgcga cgcgcagctc gtcggggagc tcgatgaact
     2281 cgccgtggat gtagcaccag ccccagtcct cgtcgggctc gaacgactcg atgatcgggt
     2341 gcccggtggt gtggaagtgc gccgtcgcgt gccggcccgg cgaggagtcg cagcagccga
     2401 cgtggccgca cgacaggcag atccgtagat ggacccaggt caggccgagc tgcaggcact
     2461 ccaggcagcc gccgctcagc ggctcgacgc gccgcgcggt gtagaggtgc ggatcgtgct
     2521 cggtgttcat gtcggtcctc cggatccggt ccgctcggtc ccacgtggga cgctgccccc
     2581 cgccccgccg gcggagccgt cggggtcccc accccccgtc tcgctgcgct cgaactcggc
     2641 ccggttccag ccggccaggt tcgcgccggc ctcgtacgtg ctctggcaga actccaggat
     2701 cttcgcgtgc gggtcctgct c
//
LOCUS       DASVOR010000319         1790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148963,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000319 DASVOR010000000
VERSION     DASVOR010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1790
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148963"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VF516_06650"
     CDS             complement(<1..1435)
                     /locus_tag="VF516_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107391.1"
                     /translation="MLLVVLAAGGVAHAQMGLPNGATIVFEHRQIFEGGKFVEPTAGT
                     QDVLHYFNLAHCNCAQANIGNANSAGWFRYNVHEIPASGLTVPFSLYAGTSCDNAANR
                     PGGSAPTCNLLVTLNDLDARLNRTGGEFEPFNLFQVVTANSLTPPDTCPTVDGVTNSI
                     YVLVDTAGGTNFNYSASQLAGGAPGDATTGAIDTKPPPLPTTKLKATPNEGRIDISWT
                     PPTNNNTDIAYYQALCAVDAPNTPPARAQTNSPLYVTTQATCPGASIPAAILTVEQPM
                     PTPLDNGEAEVAMPGGAFGALDPKYICGEVDSGTASSLAITGLENGTAYQVILVSIDR
                     HGNFSATYFDHTITPHLVTDFWEDLHDHGSKANGGLCLLAETYGNDSALTRALRAFRD
                     DTLGGSRIGRWLGRAYYATLGRLGGLVHGSIAARVVAGIALAPLVALALLWHWLGLPV
                     VLGLLAAAWWFRRRRLAATGAPRWRLPR"
     gene            complement(1588..>1790)
                     /locus_tag="VF516_06655"
     CDS             complement(1588..>1790)
                     /locus_tag="VF516_06655"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEX8107392.1"
                     /translation="AAVTPRELATQLVARGSGVAPQVGELTELYYAAEWGRRSDPAAE
                     HRAGALASEIRATLRAIRHAPR"
BASE COUNT          253 a          589 c          672 g          276 t
ORIGIN      
        1 agcggggcag tcgccagcgc ggggccccgg tcgccgcgag gcgccgccgg cggaaccacc
       61 acgcagcggc gagcagaccg agcacgacgg gcaggccgag ccagtgccag agcagcgcga
      121 gcgcgaccag cggcgcgagc gcgatgccgg cgaccacgcg cgccgcgatc gagccgtgga
      181 cgaggccgcc gagccgtccc agcgtcgcgt agtacgcgcg gccgagccag cgcccgatcc
      241 gcgagccgcc cagcgtgtcg tcgcggaacg cgcgcagggc gcgggtcagc gcgctgtcgt
      301 tgccgtaggt ctcggcgagc aggcacagcc cgccgttggc cttgctgccg tggtcgtgca
      361 ggtcctccca gaagtcggtg accaggtgcg gcgtgatcgt gtggtcgaag taggtggccg
      421 agaagttccc gtggcgatcg atcgacacca ggatcacctg gtacgcggtc ccgttctcca
      481 gccccgtgat cgcgagcgag ctcgcggttc cgctgtcgac ctcgccgcag atgtacttgg
      541 ggtcgagcgc gccgaacgcg ccgccgggca tcgcgacctc cgcctcgccg ttgtcgagcg
      601 gggtcggcat cggctgctcg acggtcagga tggcggccgg gatgctggcg cccggacagg
      661 tcgcctgggt cgtgacgtac agcggactgt tggtctgcgc gcgcgccggc ggggtgttgg
      721 gcgcgtcgac cgcgcacagc gcctggtagt acgcgatgtc ggtgttgttg ttggtcggcg
      781 gggtccacga gatgtcgatc cggccctcat tgggcgtcgc cttgagcttc gtggtcggca
      841 gcggaggagg tttggtgtcg atcgccccgg tggtcgcgtc gccgggcgca cctccggcga
      901 gctggctcgc ggagtagttg aagttcgtgc cacccgcggt atcgaccagc acgtagatcg
      961 agttggtcac gccgtcgacc gtcgggcaag tgtccggcgg ggtgaggctg ttggcggtca
     1021 cgacctggaa caggttgaac ggctcgaact cgcccccggt tctattcagc ctggcatcga
     1081 ggtcattcaa ggtcacgagc aagttgcagg tcggagcgct gccgccgggg cggttcgcgg
     1141 cgttgtcgca gctggtaccg gcatacagcg agaacgggac ggtcaggccc gacgctggga
     1201 tctcgtgaac gttgtagcgg aaccacccgg ccgagttcgc gttgccgatg ttggcctggg
     1261 cgcagttgca gtgcgccagg ttgaagtagt ggagcacgtc ctgggtcccg gccgtcggct
     1321 cgacgaactt gccgccttcg aagatctggc ggtgctcgaa cacgatggtg gccccgttgg
     1381 gcaggcccat ctgggcgtgt gcgacgccgc ctgcagcaag caccaccagg agcacggcca
     1441 caggaacgaa tcgcgtcatc gctgctgctc tttgcaaggg aaaggccgtc ggggaaccga
     1501 cgatcatcta gctatcacgc ggggatgggc gcatcaacca aaggcgctcc ggctgccaaa
     1561 ctggcacgtg tccggcgcgc cgggatgtca gcgaggcgcg tggcggatcg cccggagcgt
     1621 ggcgcggatc tcgctggcca gcgccccggc gcgatgctcg gcggccgggt cgctccggcg
     1681 gccccactcg gcggcgtagt acagctcggt cagctcgccg acctgcggcg ccaccccgct
     1741 gccacgcgcg accagctggg tcgccagctc gcgcggcgtc accgccgcct
//
LOCUS       DASVOR010000320         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_441234,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000320 DASVOR010000000
VERSION     DASVOR010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441234"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF516_06660"
     CDS             complement(<1..424)
                     /locus_tag="VF516_06660"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8107393.1"
                     /translation="MTEYRNPKPTVDIVIELADAPGQIVFIVRRNEPRGLALPGGFVD
                     EGEWVADAAVREAREETGLDVELTELFHVYSDPRRDPRQHTISAVFLARAAGRPVGGD
                     DAAECIVCAPDAPPQPLVFDHATIVADYLAYRRRGVRPP"
     gene            complement(421..>1693)
                     /locus_tag="VF516_06665"
     CDS             complement(421..>1693)
                     /locus_tag="VF516_06665"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX8107394.1"
                     /translation="PPDPGAPRAAAAAPAVSGGKNVLILFVDTLRFDRLGIAGYRRDG
                     KSLTPRLDALAAQSVLFTHAYAQAPNTPRSVPSFWTSRYSSQVKVDKKFKDYATVLDD
                     NDTLFEALSAGGFTTIGETSHFYFCDRKRYPKTCPDVVSWMKSNITQGATEWDNTGAL
                     NIPESNHDTAGPRVVKKTIGRFGELAKADTRFAMLVHLFEPHSTYMEHDGFRYTERGT
                     AGLIQKYDYEIAFEDGLIGQLLDALDATGLAKTTTVVVMADHGEAFGIHQSGGKPAFF
                     HGDTLYRELIHVPLMFRVPGAKPCRRDDVVQLIDMAPTITALFGVAAPASWQGRSLVP
                     ALACQPLEPRPAFAELLPAPEWDHEAKSMVSADGKHHVYYRISESKWEIYDLDRDPDE
                     KSSLADSPGARELEQQLAAWIEGPLAAGGGK"
BASE COUNT          226 a          590 c          611 g          266 t
ORIGIN      
        1 gcggcggccg gaccccacgc cgccggtacg cgaggtagtc ggcgacgatc gtcgcgtggt
       61 cgaacaccag cggctggggc ggggcgtccg gcgcgcacac gatgcactcg gccgcgtcgt
      121 cgccgccgac cggccggccc gccgcgcgcg cgaggaacac cgccgagatc gtgtgctgcc
      181 gcggatcgcg ccgcggatcg gagtagacgt ggaacagctc ggtgagctcg acgtcgagcc
      241 cggtctcctc cctggcctcg cggaccgcgg cgtctgccac ccactcgccc tcgtcgacga
      301 acccgccggg cagggcgagc ccgcgcggct cgttgcggcg cacgatgaac acgatctggc
      361 ccggcgcatc ggcgagctcg atcacgatgt cgacggtcgg cttggggttc ctgtactcgg
      421 tcacttgcca cctcccgcag cgagcgggcc ctcgatccac gccgcgagct gctgctcgag
      481 ctcgcgcgcg ccgggcgagt cggcgaggct ggacttctcg tcgggatcgc ggtcgagatc
      541 gtagatctcc cacttcgact cgctgatccg gtagtagacg tggtgcttgc cgtcggcgga
      601 caccatcgac ttggcctcgt gatcccactc gggcgccggc agcagctccg cgaacgcggg
      661 ccgcggctcg agcggctggc acgccagcgc cggcaccagg ctccggccct gccagctcgc
      721 cggtgcggcc acgccgaaca gcgccgtgat cgtcggcgcc atgtcgatca gctggaccac
      781 gtcgtcgcgc cggcacggct tggcgcccgg cacccggaac atcagcggca cgtggatcag
      841 ctcgcggtac agcgtgtcgc cgtggaagaa cgccggcttg ccgccggact ggtggatgcc
      901 gaacgcctcg ccgtgatcgg ccatcacgac gaccgtcgtg gtcttggcca gcccggtggc
      961 gtcgagcgcg tcgagcagct ggccgatcag cccgtcctcg aacgcgatct cgtagtcgta
     1021 cttctggatc agccccgcgg tgccgcgctc ggtgtagcgg aagccgtcgt gctccatgta
     1081 cgtcgagtgc ggctcgaaca ggtggacgag catcgcgaac ctggtgtcgg ccttggcgag
     1141 ctcgccgaac cgcccgatcg tcttcttgac gacccgcggg ccggcggtgt cgtggttgga
     1201 ctcggggatg ttgagcgcgc cggtgttgtc ccactcggtg gcgccctgcg tgatgttgct
     1261 cttcatccag ctcaccacgt cggggcaggt cttggggtag cgcttgcggt cgcagaagta
     1321 gaagtgcgag gtctcgccga tcgtggtgaa gccgcccgcc gacagcgcct cgaacagcgt
     1381 gtcgttgtcg tcgagcacgg tcgcgtagtc cttgaacttc ttgtcgacct tgacctgcga
     1441 cgagtagcgc gaggtccaga acgatggcac cgagcgcggc gtgttgggcg cctgggcgta
     1501 ggcgtgggtg aacagcaccg actgcgcggc gagcgcgtcg agccgcggcg tgagcgactt
     1561 gccgtcgcgc cggtaccccg cgatcccgag ccggtcgaac cgcagcgtgt cgacgaagag
     1621 gatcagcacg ttcttgccgc cgctcaccgc gggcgcggcc gcggcggccc tgggcgcccc
     1681 ggggtcgggc ggc
//
LOCUS       DASVOR010000321         5143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382825,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000321 DASVOR010000000
VERSION     DASVOR010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382825"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1567)
                     /locus_tag="VF516_06670"
     CDS             complement(<1..1567)
                     /locus_tag="VF516_06670"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEX8107395.1"
                     /translation="MERNMTHHVRIFDTTLRDGEQSPGATMTASEKLEVARALARLGV
                     DVIEAGFPAASPDDLAAVRAIAAEVGAVGAVGAVGGAGGAGGPPIICALARAARPDID
                     AAWQGVRAAARPRIHTFLATSDLHLEHKLRMSRADVLARVADMVAYARSLCGDVEFSA
                     EDAGRSDPGFLHAVLEVAIRAGATTLNIPDTVGYTTPDEFGALIARIRAEVPGIDGVV
                     LSVHCHDDLGLATANTLAGLIAGARQAEVTINGIGERAGNSSLEEIVMAIHTRAAKLG
                     LATGIDNTQLTAVSRLVSTATGFVVPPNKAIVGANAFAHESGIHQDGMLKHQATYEIM
                     RPETVGAAQTRLVLGKHSGRAALAARLAELGTPLSGVALDRVFARFKALTDRKKQVTD
                     ADLEALVSDEATAAGEHDELALDGLQVSCGTMGMPTATLRLRRPDGGLAVQAAIGTGP
                     VDAAYKAIDAIVARWDRGGAAARGELVEFAIHAVTEGIDALGHVTVRVREGAERGVAP
                     HGEARSRVFHGAEI"
     gene            complement(1887..2900)
                     /gene="ilvC"
                     /locus_tag="VF516_06675"
     CDS             complement(1887..2900)
                     /gene="ilvC"
                     /locus_tag="VF516_06675"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEX8107396.1"
                     /translation="MAKIFYDKDADLGLIRGSKVAIIGYGSQGHAHALNLQDSGVAVK
                     VGLRPTSQGVARARAAGLEVGAVAEVSAWADVVMLLAPDTAQPRIYRDAVAPHLAPGK
                     TQMFAHGFNIRYGTIAPRGDVDVSMIAPKGPGHRVRETFQAGDGVPALLAVHQDASGR
                     ARALALSYAAAIGAGRAGVLETTFTEETETDLFGEQAVLCGGVSALVKAGFETLVGAG
                     YQPEVAYFECLHELKLIVDLMYRGGLDYMRYSVSDTAEYGDYVAGPHIVDGHTRAAMK
                     RLLAEIQDGSFARRWIEENETGRAAFLAERARQRAHPIEQVGAALRAMMPFLDPVTVD
                     PGE"
     gene            complement(2937..3479)
                     /gene="ilvN"
                     /locus_tag="VF516_06680"
     CDS             complement(2937..3479)
                     /gene="ilvN"
                     /locus_tag="VF516_06680"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF008864.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEX8107397.1"
                     /translation="MTESRLRTFIAYVEDLPGVLNRITSLFRRRSYNIESLAVGRTHE
                     PGVSRMTLVLEADDDGARRIEANLYKLVNVLRVEDTAQRATVARELALVKVRANAGSR
                     PSVMQICEVFRARVIDVGPEALICEITGQAAKIDGFVDVLQPFGVLEMVRTGAVAMVR
                     SAEASQRALLDEPPGARDAA"
     gene            complement(3521..>5143)
                     /gene="ilvB"
                     /locus_tag="VF516_06685"
     CDS             complement(3521..>5143)
                     /gene="ilvB"
                     /locus_tag="VF516_06685"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEX8107398.1"
                     /translation="MADGYARASGKVGVAIATSGPGATNLVTGIATAMLDSVPIVCIT
                     GQVPSHVIGTDAFQETDITGVTLPITKHNYLVSRADQIAPTIREAFYIARSGRPGPVL
                     VDITKDAQQRPAEVAWSETPVSVSLRGYRPEHTAGDADLARAVAMIDAAKRPVILAGH
                     GIVKAGASALLGELVERTGIPVAATLLGLGGFPASHPLALGLMGMHGEAWVNHAIQEA
                     DLLLALGMRFDDRVTGNPATFAVAARKIHCELDPAEINKNVRADVALVGDVGDTLRAL
                     LPRVARVEHRACAERRACAERRACAERRACAERRAWIDRIAELKGDSAVRDIQRMPHH
                     GRLFAAHVLHDLWRITEGKALVVTDVGQHQMWEAQYYKHDHPRKLVTSGGLGTMGFAL
                     PAAIGARFADPAAEIWVVAGDGGFQMTACELSTAAQEGIKLCVAIINNGYLGMVRQWQ
                     EFFYDGRYSATPLRSPDFVKLAEAHGLTGLRVTRREEVEAAVQAARAAPGTVVIEFRV
                     EQEDAVYPMVPAGADLRDMIRRPSPIVETADD"
BASE COUNT          674 a         1827 c         1877 g          765 t
ORIGIN      
        1 cgatctcggc gccgtggaac acgcgcgagc gcgcctcgcc gtgcggcgcc acgccgcgct
       61 ctgcaccctc gcggacccgc acggtgacgt ggccgagcgc gtcgatgccc tcggtcaccg
      121 catggatcgc gaactcgacc agctcgccgc gcgccgccgc gccgccgcga tcccagcgcg
      181 cgacgatcgc gtcgatcgcc ttgtacgcgg cgtcgaccgg gccggtgccg atcgcggcct
      241 gcaccgcgag cccaccgtcg ggccggcgca gccgcagggt cgcggtcggc atgcccatcg
      301 tgccgcagct gacctgcagg ccgtcgagcg cgagctcgtc gtgctcgccg gcggcggtcg
      361 cctcgtcgct gaccagcgcc tcgaggtcgg cgtcggtgac ctgcttcttg cgatcggtca
      421 gcgccttgaa ccgcgcgaac acgcggtcga gcgcgacgcc ggacagcggc gtgccgagct
      481 cggccaggcg ggccgccagc gccgcgcggc ccgagtgctt gccgagcacg agccgggtct
      541 gggccgcgcc gacggtctcc ggccgcatga tctcgtaggt cgcctggtgc ttgagcatgc
      601 cgtcctgatg gatcccggac tcgtgggcga acgcgttggc gccgacgatc gccttgttgg
      661 gtgggacgac gaacccggtg gcggtgctga ccagccggct gaccgcggtg agctgggtgt
      721 tgtcgatccc ggtggcgagt ccgagcttcg ccgcgcgggt gtggatcgcc atgacgatct
      781 cctcgagcga gctgttgccg gcgcgctcgc cgatgccgtt gatcgtgacc tcggcctggc
      841 gcgctccggc gatcagaccg gccagcgtgt tggcggtggc gagcccgagg tcgtcgtggc
      901 agtggaccga cagcacgacg ccgtcgatcc cggggacctc ggcgcggatc cgcgcgatca
      961 gcgcgccgaa ctcgtccggc gtggtgtagc cgacggtgtc ggggatgttg agcgtggtcg
     1021 cgccggcgcg gatcgcgacc tcgagcaccg cgtgcaggaa gccgggatcg ctgcgcccgg
     1081 cgtcctcggc gctgaactcg acgtcgccgc acagcgagcg cgcgtacgcg accatgtcgg
     1141 cgacccgcgc cagcacgtcg gcgcgcgaca tccgcagctt gtgctcgagg tggagatccg
     1201 acgtcgcgag gaacgtgtgg atccgcggcc gcgccgccgc ccgcacgccc tgccacgcgg
     1261 cgtcgatgtc gggccgcgcc gcgcgggcga gcgcgcagat gatcggcggg ccgccggccc
     1321 cgccggcccc accgaccgct ccgaccgctc cgaccgctcc gacctccgcg gcgatcgcgc
     1381 ggaccgcggc gaggtcgtcg ggcgacgccg ccgggaaccc ggcctcgatg acatcgaccc
     1441 cgagccgggc cagcgcgcgg gcgacctcga gcttctcgga cgcggtcatg gtggcgccgg
     1501 gcgattgctc gccgtcgcgc agcgtggtgt cgaagatccg aacgtggtgg gtcatgttgc
     1561 gctccacggc tcgatcaaaa ggtatcgatc acaggggttg agacttgcct cgagtgtgcc
     1621 tgcgtccggt cgacgtcgac ctgggccttg accttgacgg ccatcgttga ggttgacgtg
     1681 gccgtggacg ttgagcccat cgtcgacctc caccttgacc atcggtcgcg attcttcgac
     1741 gagaattcgc agacaccaga aggtccacgt acaaggtcga aggtggagtc gacgtcgccg
     1801 tcgccgttca ggtcaacgtc ttgcgacaac gtcaaggtcg acctcaacgt cttcgcaagg
     1861 acattcccgg ccttcatcga ctctcttcac tcgccggggt cgacggtgac gggatcgagg
     1921 aacggcatca tggcgcgcag cgcggcgccg acctgctcga tcgggtgcgc gcgctggcgg
     1981 gcgcgctcgg ccaggaacgc ggcgcgcccg gtctcgttct cctcgatcca gcgccgcgcg
     2041 aacgagccgt cctggatctc ggcgagcagc cgcttcatcg ccgcgcgggt gtggccgtcg
     2101 acgatgtgcg ggcccgcgac gtagtcgccg tactccgcgg tatcgctgac cgagtagcgc
     2161 atgtagtcca ggccgccgcg gtacatcagg tcgacgatca gcttgagctc gtgcaggcac
     2221 tcgaagtacg cgacctcggg ctggtagccg gcgccgacca gggtctcgaa cccggccttg
     2281 accagcgcgc tgaccccgcc gcacagcacc gcctgctcgc cgaacagatc ggtctcggtc
     2341 tcctcggtga acgtggtctc caggacgccg gcgcgcccgg cgccgatcgc cgcggcgtac
     2401 gacagcgcga gcgccctggc ccggccgctg gcatcctggt gcaccgcgag cagcgccggc
     2461 acgccgtcgc cggcctggaa ggtctcgcgg acgcgatggc cggggccctt gggcgcgatc
     2521 atcgacacgt cgacgtcgcc gcgcggcgcg atcgtgccgt agcggatgtt gaagccgtgc
     2581 gcgaacatct gcgtcttgcc gggcgcgagg tggggcgcga ccgcgtcgcg gtagatccgc
     2641 ggctgcgcgg tgtcgggcgc gagcagcatc acgacgtcgg cccacgcgct gacctcggcg
     2701 accgccccga cctcgaggcc ggcggcgcgc gcccgggcga cgccctggct ggtcggccgc
     2761 agcccgacct tgacggcgac gccgctgtcc tggaggttga gcgcgtgggc gtggccctgc
     2821 gagccgtagc cgatgatcgc gaccttgctg ccgcggatca ggccgaggtc ggcgtccttg
     2881 tcatagaaga tctttgccat gggttgattc ctgttctgga agcctgaagt tggggttcac
     2941 gccgcgtcgc gcgcgccggg tggctcgtcg agcagcgcgc gctgcgaggc ctcggcgctg
     3001 cgcaccatcg cgaccgcgcc ggtccgcacc atctcgagca cgccgaacgg ctggagcacg
     3061 tcgacgaagc catcgatctt ggcggcctgc ccggtgatct cgcagatcag cgcctcgggg
     3121 ccgacgtcga tcacgcgggc gcggaacacc tcgcagatct gcatgacgct cggccgcgat
     3181 ccggcgttgg cccggacctt gaccagcgcg agctcgcgcg ccacggtcgc gcgctgcgcg
     3241 gtgtcctcga cccgcagcac gttgaccagc ttgtacaggt tggcctcgat ccggcgcgcg
     3301 ccgtcgtcgt cggcctccag caccagcgtc atgcgcgaca cgccgggctc gtgggtccgg
     3361 ccgaccgcga gcgactcgat gttgtagctg cggcggcgga acagcgaggt gatccggttg
     3421 aggacaccgg gcagatcttc gacataggcg atgaacgtcc gtagacggct ctcggtcatg
     3481 acgggctccg tggctggggg cggtgacagc gaacagcggt tcagtcgtcg gcggtctcga
     3541 cgatcgggct cgggcggcgg atcatgtcgc ggaggtcggc gccggcgggg accatcgggt
     3601 agaccgcgtc ctcctgctcg acgcgaaact cgatgacgac ggtgccgggg gcggcgcgcg
     3661 ccgcctggac cgcggcctcg acctcctcgc ggcgggtcac gcgcaggccg gtcaggccgt
     3721 gggcctcggc gagcttgacg aagtccgggc tgcgcagcgg cgtcgccgag tagcggccgt
     3781 cgtagaagaa ctcctgccac tgccggacca tgccgaggta gccgttgttg atgatcgcga
     3841 cgcacagctt gatgccctcc tgggccgcgg tcgacagctc gcacgcggtc atctggaatc
     3901 cgccgtcgcc ggcgaccacc cagatctcgg cggcggggtc ggcgaaccgc gcgccgatcg
     3961 cggcgggcag cgcgaacccc atcgtgccga gcccgcccga ggtcacgagc ttgcgcgggt
     4021 gatcgtgctt gtagtactgg gcctcccaca tctggtgctg gccgacgtcg gtgacgacca
     4081 gcgccttgcc ctcggtgatc cgccacagat cgtgcagcac gtgggcggcg aacagccggc
     4141 cgtggtgcgg catgcgctgg atgtcgcgca ccgcggagtc gcccttgagc tcggcgatcc
     4201 ggtcgatcca ggcccgacgc tcggcacagg cccgacgctc ggcacaggcc cgacgctcgg
     4261 cacaggcccg gcgctcggca caggctcggt gctcgacgcg cgcgacccgc ggcagcagcg
     4321 cgcgcagcgt gtcgccgacg tcgccgacca gcgcgacgtc ggcccggacg ttcttgttga
     4381 tctccgccgg atcgagctcg cagtggatct tcctggcggc caccgcgaac gtcgccggat
     4441 tgccggtgac gcggtcgtcg aaccgcatgc cgagcgcgag caggaggtcg gcctcctgga
     4501 tcgcgtggtt gacccaggcc tcgccgtgca tgcccatcag gccgagcgcc agcgggtgcg
     4561 acgcggggaa cccgccgagg ccgagcagcg tcgcggcgac cgggatcccg gtgcgctcga
     4621 cgagctcgcc gagcagcgcg ctcgcccccg ccttgacgat gccgtggccg gcgaggatca
     4681 ccgggcgctt cgcggcgtcg atcatcgcca ccgcgcgcgc gaggtcggcg tcacccgcgg
     4741 tgtgctcggg ccggtagccg cgcaggctga cgctgaccgg tgtttcgctc cacgcgacct
     4801 cggccgggcg ctgctgcgcg tccttggtga tgtcgaccag caccggcccg gggcgtcccg
     4861 accgggcgat gtagaacgcc tcgcggatcg tcggcgcgat ctggtcggcg cggctgacca
     4921 ggtagttgtg cttggtgatc ggcagcgtga cgccggtgat gtcggtctcc tggaacgcgt
     4981 cggtgccgat cacgtgcgac ggcacctggc cggtgatgca gacgatcggc accgagtcga
     5041 gcatcgcggt cgcgatcccg gtcaccaggt tggtcgcgcc cgggcccgag gtcgcgatcg
     5101 cgaccccgac cttgcccgac gcgcgcgcgt agccgtcggc cat
//
LOCUS       DASVOR010000322         2601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_148997,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000322 DASVOR010000000
VERSION     DASVOR010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2601
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148997"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF516_06690"
     CDS             complement(<1..213)
                     /locus_tag="VF516_06690"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX8107399.1"
                     /translation="MRRLAVLCVYAVVASVTLAHAGARRTLEPVALRKKPGEKEAVVA
                     RLPAGAEVTVLAIDGRWLRVRAGGVEG"
     gene            417..923
                     /locus_tag="VF516_06695"
     CDS             417..923
                     /locus_tag="VF516_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8107400.1"
                     /translation="MSFAILVTASPWVAGCDGSLREKVGQSDIDASVLMTIQPPKCDP
                     PATAPDSGACTGGAQPGADCLMCHHQGGPAAPYTFAGTLYDAAGAQPVAGATIYVQDS
                     AGNIATAITRPNGNFFTADGFVQYPAKAFVSRCPDVLEMVGPVDQRTGANCNTSGCHT
                     AGFRIHLP"
     gene            931..2592
                     /locus_tag="VF516_06700"
     CDS             931..2592
                     /locus_tag="VF516_06700"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8107401.1"
                     /translation="METTVPILIGLGTVLLLGLGAILDDRRARLRRYDARDEPALAVP
                     LLHSINDDRCIGCEACVDVCPTEVLDLVDHKVRVARFSDCVQCEQCANACPTQALVMY
                     RAGAAPRMLTVPEIDEHFQTGVPGQYLIGEVAGKPLVKNAANLGRAVIEHIARDLAGK
                     PGAGADTLDVAIVGSGPGGLSAGLACIKHRLSCLVLEKEHVIASTVARYPKGKHFMAE
                     PCSAQNVSYLPVFDGTKEQLVAAWTQLVQAVQLPIRLGEAVETVQRGDDGVFTIQTTV
                     ARYRARAVVLATGLRGKPRLLAVPGANLEKVQSLLDDPAEFAGEHALVVGGGDSAVEG
                     AIALAEAGATVTLSYRGDGFKRCKQGNQKRLAEMIAGQRLTVLLESNVTRFTPDAVTI
                     ACKDRDGSREVTIPNQRAFVLIGADTPIAWLEANQVRFVERPHLYALGSTEDVVRRFA
                     PGATECPRSPEEALAVLQDRPAPRQPVRRMKSVAHHLREEIWDVVTSVSAAFRLSDLE
                     AEHDARRAATRAPARGVTSLDDAPTEEMVSLRTLEATAARKRLAR"
BASE COUNT          377 a          978 c          890 g          356 t
ORIGIN      
        1 gccctccacg cctcccgccc gtacccgcag ccagcggccg tcgatcgcga gcacggtgac
       61 ctcggcgccg gccggcagcc gcgcgaccac cgcctccttc tctcccggct tcttgcgcag
      121 cgcgaccggc tccagcgtgc ggcgcgcgcc ggcgtgggcc agcgtcaccg acgccacgac
      181 ggcgtacaca cacagcacgg cgagccttcg catgtggagc gaaggtctct agcagctcgc
      241 gcaccaggtc tttcgcattc gcaaccacgc ggacttgcct gtcctgatcg tgcacaggcg
      301 aggtcgtttg tacgagcttg gtactttttg gcgatttcga gcgaccaaaa cacgcacact
      361 tcgcacggcg gatccgggaa tacgcgttgc agccatgtcg gcgaatgcac cgtggaatgt
      421 cgttcgcgat cctcgtcacg gcgagtccgt gggtcgccgg gtgcgatggc tcgctgcgcg
      481 agaaggtcgg ccagtccgac atcgacgcgt cggtgctgat gacgatccag ccgccgaagt
      541 gcgatccacc ggcgaccgcc cccgacagcg gcgcctgcac cggcggcgcc cagcccggcg
      601 ccgactgcct gatgtgccac caccagggcg gccccgcggc gccctacacg ttcgccggca
      661 cgctgtacga cgccgcgggc gcacagccgg tcgccggcgc gacgatctac gtccaggact
      721 cggccggcaa catcgccacg gcgatcaccc gccccaacgg caacttcttc accgccgacg
      781 gcttcgtcca gtacccggcc aaggcgttcg tctcgcggtg tccggacgtg ctcgagatgg
      841 tcggcccggt cgatcagagg accggcgcca actgcaacac ctcgggctgc cacaccgcgg
      901 gcttccgcat ccacctcccg tgagcccctg atggagacca ccgtaccgat cctgatcggc
      961 ctgggcaccg tgctcttgct cggcctgggc gccatactcg acgaccgccg cgcccggctg
     1021 cggcggtacg acgcgcgcga cgagccggcg ctcgccgtgc cgctcctgca ctcgatcaac
     1081 gacgaccgct gcatcggctg cgaggcgtgc gtcgacgtgt gcccgaccga ggtgctcgac
     1141 ctggtcgatc acaaggtccg ggtcgcgcgg ttctccgact gcgtgcagtg cgagcagtgc
     1201 gccaacgcct gtccgaccca ggcgctggtg atgtaccgcg caggcgctgc gccgcgcatg
     1261 ctgaccgtgc cggagatcga cgagcacttc cagaccggcg tgcccggcca gtacctgatc
     1321 ggcgaggtcg ccggcaagcc gctggtcaag aacgccgcca acctcggccg cgccgtgatc
     1381 gagcacatcg cgcgcgacct cgcgggcaag cccggcgccg gcgctgacac gctcgatgtt
     1441 gcgatcgtcg gctcggggcc gggcggcctg tcggcgggcc tggcctgcat caagcacagg
     1501 ctgtcgtgcc tggtgctcga gaaggagcac gtgatcgcgt cgaccgtggc gcgctacccc
     1561 aagggcaagc acttcatggc cgagccatgc tcggcgcaga acgtgtcgta cctgccggtg
     1621 ttcgacggca ccaaggagca gctggtcgcg gcgtggaccc agctggtcca ggccgtccag
     1681 ctgccgatcc ggctcggcga ggcggtcgag accgtgcagc gcggcgacga cggcgtgttc
     1741 acgatccaga ccacggtcgc ccgctaccgc gcccgcgccg tcgtgctcgc caccggcctg
     1801 cgcggcaagc cgcggctgct cgccgtgccg ggcgccaacc tcgagaaggt ccagtcgctg
     1861 ctcgacgatc cggccgagtt cgccggcgag cacgcgctgg tggtcggcgg cggcgactcg
     1921 gcggtcgagg gcgcgatcgc gctggccgag gccggcgcca cggtgacgct gtcgtaccgc
     1981 ggcgacggct tcaagcgctg caagcagggc aaccagaagc ggctcgccga gatgatcgcc
     2041 ggccagcggc tcaccgtgct gctcgaatcc aacgtcacgc gcttcacccc ggacgcggtg
     2101 accatcgcgt gcaaggaccg cgacgggagc cgcgaggtca cgatccccaa ccagcgcgcg
     2161 ttcgtgctga tcggcgccga cacgccgatc gcgtggctgg aggccaacca ggtccggttc
     2221 gtcgagcgcc cccacctcta cgcgctgggc tcgaccgagg acgtcgtgcg gcggttcgcc
     2281 cccggcgcca ccgagtgccc gcgctcgccc gaggaggcgc tcgccgtcct gcaggaccgc
     2341 ccggcgccgc gccagccggt gcgccgcatg aagtcggtcg cccaccacct ccgcgaggag
     2401 atctgggacg tggtgaccag cgtcagcgcg gcgttccggc tgtccgacct ggaggccgag
     2461 cacgacgcgc ggcgcgccgc gacccgcgcg cccgcgcgcg gcgtgacgtc cctcgacgac
     2521 gccccgaccg aggagatggt gtcgctgcgc acgctcgagg cgaccgcagc gcgcaagagg
     2581 ctcgcccgat gatggcggtc c
//
LOCUS       DASVOR010000323         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_61471,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000323 DASVOR010000000
VERSION     DASVOR010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61471"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VF516_06705"
     CDS             complement(<1..492)
                     /locus_tag="VF516_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8107402.1"
                     /translation="MRRSLAAVAVLGLAGCGPPDGDTFGPVAPPDRIDATHFTWCSQG
                     EPDHLDPTLAWSTASIQLVVLLFDGLTTYGPAGQPVPGLATDWEIAGDLRRFTFHLRR
                     DARWSSGRPVTAYDVAHSAFRVAHPLTGSSNADWLAPIRGATAYLEQRALVLRRAVGP
                     YRAG"
     gene            693..>1520
                     /locus_tag="VF516_06710"
     CDS             693..>1520
                     /locus_tag="VF516_06710"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX8107403.1"
                     /translation="MADKHKSKHIKLTSHPGAGSAKPIPIHWGAADPHTRGPIIATLT
                     TPEHRNVIGTHSGSYAVYRALAIAAKALDPQHVPDLTNTTPTDPIGPYPAWHDPEKIV
                     SIDPYGAVVAEAFRDYLQKGYDVRPTIAVTKAHIDLPEIVDAIEARRLTVDGKIVKSR
                     REIAVTKAATEPVWYLPGVAKRFDCGESELRRTLFEQTGGMFPELVTRGDLDVFLPPI
                     GGQTIYMFGDPHKLADPAVTLTARVHDECNGSDVFGSDICTCRPYLAHAIEECIRGAQ
                     "
BASE COUNT          243 a          577 c          510 g          190 t
ORIGIN      
        1 accggcgcgg tacggcccca ccgcgcgccg cagcaccagc gcgcgctgct cgaggtacgc
       61 ggtcgcgccg cggatcggcg ccagccagtc cgcgttcgac gacccggtca gcgggtgcgc
      121 cacccggaac gcgctgtgcg ccacgtcgta cgcggtgacc ggccggccgc tcgaccaccg
      181 cgcgtcgcgg cgcaggtgga acgtgaaccg gcgcaggtcg ccggcgatct cccagtcggt
      241 cgccagcccc ggcaccggct ggcccgccgg cccgtaggtc gtcagcccgt cgaacagcag
      301 caccaccagc tggatcgacg ccgtgctcca cgccagcgtc ggatcgaggt ggtccggctc
      361 gccctggctg caccacgtga aatgcgtcgc atcgatccgg tccggcggcg ccaccgggcc
      421 gaaggtgtcg ccgtcgggcg ggccgcatcc ggcgagcccg agcacagcga ccgcggcgag
      481 cgagcgacgc accgccctca gtgtaccgcg acgagccgct gctcctccgc ggccggcgcg
      541 atcgggaaca gggactgcgc agccaccatg acgcaagcgt accagctccc ggcaggtcgt
      601 cctcgggcgg aaggccccct cggtgcggtt cctttaccag catcgggctt cgtgccactg
      661 ttgatgcttt cccgcacagg accacctgag cgatggccga caagcacaag tccaagcaca
      721 tcaagctgac gtcgcacccc ggcgcgggca gcgcgaagcc gatcccgatc cactggggcg
      781 cggccgaccc gcacacccgc gggccgatca tcgcgacgct gaccacgccc gagcaccgca
      841 acgtgatcgg cacccactcg ggtagctacg ccgtgtaccg cgcgctcgcg atcgccgcca
      901 aggcgctcga tccgcagcac gtcccggacc tgaccaacac caccccgacc gatccgatcg
      961 gcccgtaccc ggcgtggcac gatccggaga agatcgtgtc gatcgacccg tacggcgcgg
     1021 tcgtcgccga ggcatttcgc gactacctgc agaagggcta cgacgtccgg ccgaccatcg
     1081 cggtgaccaa ggcgcacatc gacctgcccg agatcgtcga cgcgatcgag gcgcggcggc
     1141 tcaccgtcga cggcaagatc gtcaagtcgc ggcgcgagat cgcggtgacc aaggcggcga
     1201 ccgagccggt gtggtatctg cccggcgtcg ccaagcggtt cgactgcggc gagagcgagc
     1261 tgcgccgcac gctgttcgag cagaccggcg gcatgttccc cgagctggtc acccgcggcg
     1321 atctcgacgt gttcctgccg ccgatcggcg gccagacgat ctacatgttc ggcgatccgc
     1381 acaagctcgc cgacccggcg gtcacgctga ccgcgcgggt ccacgacgag tgcaacggct
     1441 cggacgtgtt cggctcggac atctgcacct gccggccgta cctggcgcac gcgatcgagg
     1501 agtgcatccg cggcgcacag
//
LOCUS       DASVOR010000324         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_3086,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000324 DASVOR010000000
VERSION     DASVOR010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3086"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1550)
                     /locus_tag="VF516_06715"
     CDS             complement(78..1550)
                     /locus_tag="VF516_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8107404.1"
                     /translation="MQPTKILVADDEQNLRRVLVAMLRRDGHDVVQAASGVEAIERLA
                     DVDVVITDLRMPGADGMEVLRTAARSYPQVPVIMITAYGSVGQAVEAIKAGAFDYIEK
                     PFEQDSIRAIVEKAIGQAVANRMAPRQTLDPVSDPDAKGRFGLVGQSSEIQNIFAVIE
                     KVADTPSTVLITGESGTGKELVAKALHENSSRKTGPFIKINCAAIPKTLMESELFGYE
                     KGAFTGATSSKPGRFELADGGTLFLDEIGEIPVEMQVKLLRAIQESEFERVGGLKTIK
                     VDVRLVTATNRDLEQEIARGNFREDLYYRLNVVPLQIPPLRKRAGDIPLLVSHIIKKF
                     NERLRKTISGIADDALAMLEAHSWPGNIRELENVLERTILFTKGDRIERTDLQLAGAS
                     EPAHAHPPAAPSSSAPSGPIALPDLDDDEPVGGEHIGSPSGSLKDIVRAETSRVEREL
                     IVKALDETGGNVTQAARLLKISRKSLQMKMKEFGLRDKEG"
BASE COUNT          222 a          624 c          637 g          306 t
ORIGIN      
        1 ccgacgggcg gcgcagtgtg cgaggcatcg cagccgctgg cagctcgcgc ggcggggctg
       61 ccgcggccgg ggccgcctca gccctccttg tcgcgcaggc cgaactcctt catcttcatc
      121 tgcaggctct tgcggctgat cttgagcagc cgcgccgcct gggtgacgtt gccgccggtc
      181 tcgtcgagcg ccttgacgat cagctcgcgc tcgacccgcg aggtctcggc gcggacgatg
      241 tccttgagcg agcccgacgg cgagccgatg tgctcgccgc cgaccggctc gtcgtcatcg
      301 aggtcgggga gcgcgatcgg tccgctcggc gccgacgagg acggcgcggc cggcgggtgg
      361 gcgtgcgcgg gctcgctggc gccggcgagc tggaggtcgg tgcgctcgat ccggtcgccc
      421 ttggtgaaca ggatcgtccg ctcgaggacg ttctccagct cgcggatgtt gccgggccag
      481 ctgtgggcct cgagcatggc gagcgcgtcg tcggcgatcc cggagatcgt cttgcgcagc
      541 cgctcgttga acttcttgat gatgtgcgag accagcagcg ggatgtcgcc ggcgcgcttg
      601 cgcagcggtg ggatctgcag cggcacgacg ttgagccggt agtacagatc ctcgcggaag
      661 ttgccgcgcg cgatctcctg ctcgaggtcg cggttggtcg cggtgaccag gcggacgtcg
      721 accttgatcg tcttgagccc gccgacccgc tcgaactcgc tctcctggat cgcgcgcagg
      781 agcttgacct gcatctcgac ggggatctcg ccgatctcgt cgaggaacag cgtgccgccg
      841 tcggcgagct cgaaccgccc cggcttgctc gaggtcgcgc cggtgaacgc gcctttctcg
      901 tagccgaaca gctcggactc catcagcgtc ttggggatcg ccgcgcagtt gatcttgatg
      961 aacggcccgg tcttgcggct cgagttctcg tgcagcgcct tggcgaccag ctccttgccg
     1021 gtgccgctct cgccggtgat cagcacggtc gacggcgtgt cggcgacctt ctcgatcacc
     1081 gcgaagatgt tctggatctc cgagctctgg ccgaccaggc cgaaccggcc cttggcgtcg
     1141 ggatcgctca ccgggtccag ggtctggcgc ggcgccatcc ggttggcgac ggcctggccg
     1201 atcgccttct cgacgatcgc gcggatcgag tcctgctcga acggcttctc gatgtagtcg
     1261 aacgcgccgg ccttgatcgc ctcgaccgcc tggccgaccg agccgtacgc ggtgatcatg
     1321 atgaccggga cctggggata cgaccgggct gccgtgcgca gcacctccat gccgtcggcc
     1381 cccggcatcc gcaggtcggt gatcacgacg tcgacgtcgg ccagccgctc gatcgcctcg
     1441 accccgctgg ccgcctgcac cacatcgtgg ccgtcgcgtc gcagcatcgc gaccagcacg
     1501 cggcgaaggt tctgctcgtc gtcggcgacc aggatcttcg tcggttgcat ggccaattac
     1561 cctaccgcat cccgcgctcg cgtgcgccgc ggggcccccg gcccaccggc cgccggcgct
     1621 actggccgcc gggggagccg gtcgcgggcg cggtggtgag ccggggcgcc gggcgcggct
     1681 tggcgttctt gacgtacgtg tcgagccagc gaacctgctc ccacagcacg tggagcgcgg
     1741 actcgcgaga gcggtagccg tgcgcctcgg cgggcagcga cacgtagcg
//
LOCUS       DASVOR010000325         3618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_178443,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000325 DASVOR010000000
VERSION     DASVOR010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3618
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178443"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..843)
                     /locus_tag="VF516_06720"
     CDS             complement(64..843)
                     /locus_tag="VF516_06720"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEX8107405.1"
                     /translation="MAIITLTTDFGTADGYVGAMKGVIARIAGSYPAPMVVDVAHDIP
                     PGDIAHAAWVVATATTEFPSGSIHVVVVDPGVGGARREVVVRARSRWYVGPDNGVFAY
                     VADRTSEAYAIENERFRAEHVSRTFHGRDVFAPTAAAIASGEDPTTAGPRVELIGRLP
                     WGLRERDVGRIVHIDRFGNLISDLPEAEAGRAVAVAGQVLPLVGTYEDVAPGQLLAYI
                     GSAGTVEIAVRDGRADRQLAVARGALVHPFTGELPARGPYR"
     gene            870..1610
                     /locus_tag="VF516_06725"
     CDS             870..1610
                     /locus_tag="VF516_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011553803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX8107406.1"
                     /translation="MNKVYPDAQAALHDVGDGAVILAGGFGLSGNPENCIRELARKQV
                     KNLTIVSNNCGTTDKGLGLLLAQDQVKRMVASYVGENKIFEQQFLSGQLEVELCPQGT
                     LAERLRAGGAGIEAFYTPTGYGTQVADGKETREINGRRCVLEHAIRGDFAIVKAWRGD
                     RWGNLVFRKTARNFNPLCAQAGKVTIVEVEQLVEVGELDPDQVHLPSIYVQRIFQGTG
                     YDKPIEQRTVRPRPGQGQGEGQAQGKRS"
     gene            1652..2287
                     /locus_tag="VF516_06730"
     CDS             1652..2287
                     /locus_tag="VF516_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HEX8107407.1"
                     /translation="MARRVAAELCDGFYVNLGIGMPTLVANYIPDGVDVVLQSENGLL
                     GIGPFPYEGDEDPDLINAGKQTVTTIRGSAFFDSAQSFAMIRGGHVDVSVLGAMQVSA
                     RGDLANWMIPGKMIKGMGGAMDLVAGSRRVIVMMEHTAKDGEPKILRDCTLPLTGVAC
                     VHTIVSDLAVIDVTGEGLVLRERAPGASAEDVANATGAPLIWTASVPEIKV"
     gene            complement(2539..2958)
                     /locus_tag="VF516_06735"
     CDS             complement(2539..2958)
                     /locus_tag="VF516_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107408.1"
                     /translation="MIFARRGRRADGERRAGSEPLTIHRARYQDVTAPVDLGRDFEQT
                     VALAPLPEPGTAKASGGGASSSAGGSTGAERDGRSSGKSSSADHDSRTVRPTSRDGGS
                     KRSGLPPKEDCQPPDKFNPYEAACHGHVCKPCPVATP"
     gene            3217..3612
                     /locus_tag="VF516_06740"
     CDS             3217..3612
                     /locus_tag="VF516_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107409.1"
                     /translation="MPRINQQALKTELAKRGFESISIKRELPGGRVEVDANKLHPVHD
                     EGGEAIYAPVPVSLSVELDGRGKVKSIAGDTPKPAAVADAARYMKTLRDSGQLAIAGG
                     GTPARGATHQIERDAQGRQVLRRKRFSII"
BASE COUNT          593 a         1237 c         1276 g          512 t
ORIGIN      
        1 ggcccacgcc atcgccaccc gccgcgagcg ccgtgatggc gagcgcgagg gcctccgctt
       61 cgctcatcga tacggccccc gcgcgggcag ctcgccggtg aacgggtgca ccagggcgcc
      121 gcgggcgacc gcgagctggc ggtcggcgcg gccatcgcgc accgcgatct cgacggtgcc
      181 ggccgacccg atgtaggcga gcagctggcc gggcgcgaca tcctcgtagg tcccgaccag
      241 cggcaggacc tggccggcga ccgcaaccgc ccggccggcc tcggcctccg ggagatcgct
      301 gatcaggttg ccgaaccgat cgatgtggac gatgcggccg acgtcgcgct cgcgcaggcc
      361 ccacggcaac cggccgatga gctcgacccg cggcccggcc gtcgtgggat cctcgccgga
      421 cgcgatcgcc gcggcggtcg gggcgaacac gtcgcggccg tggaaggtgc ggctgacgtg
      481 ctcggcgcga aaccgctcgt tctcgatcgc gtaggcctcg ctggtgcggt cggcgacgta
      541 ggcgaacacg ccgttgtccg ggccgacgta ccaccggctg cgcgcgcgca ccacgacctc
      601 gcggcgcgcc ccgccgacgc cgggatcgac gaccacgacg tggatcgagc cgctggggaa
      661 ctccgtggtc gcggtcgcca ccacccaggc cgcgtgggcg atgtcgcccg gcgggatgtc
      721 atgggcgaca tcgacgacca tcggcgcggg gtagctaccc gcgatccggg cgatcacgcc
      781 cttcatcgcc ccgacgtagc cgtcggcggt gccgaaatcc gtggtcaacg tgatgatcgc
      841 catcgccgcg agcggtaagc atatatccga tgaacaaggt ctacccggat gcgcaggcag
      901 cgctgcacga cgtcggcgac ggcgcggtga tcctcgccgg tggcttcggc ctgtcgggca
      961 accccgagaa ttgcatacgc gagctggcgc gcaagcaggt caagaacctc acgatcgtgt
     1021 cgaacaactg cggcacgacc gacaagggcc tcggcctcct gctcgcgcag gaccaagtca
     1081 agcgcatggt ggcgtcgtac gtcggcgaga acaagatctt cgagcagcag ttcctgtccg
     1141 ggcagctcga ggtcgagctg tgtccgcagg gcacgctcgc cgagcgcctg cgcgcgggcg
     1201 gcgccgggat cgaggcgttc tacacgccga ccgggtacgg cacgcaggtc gccgacggca
     1261 aggagacccg cgagatcaac ggccggcgct gcgtgctcga gcacgcgatc cgcggcgact
     1321 tcgcgatcgt caaggcgtgg cgcggcgacc gctggggcaa cctggtgttc cgcaagaccg
     1381 cgcgcaactt caacccgctg tgcgcccagg ccggcaaggt cacgatcgtc gaggtcgagc
     1441 agctggtcga ggtcggcgag ctcgaccccg atcaggtgca cctgccgtcg atctacgtcc
     1501 agcggatctt ccagggcacc ggctacgaca agccgatcga gcaacgcacg gtccggccgc
     1561 gacccggcca gggccagggc gaaggccagg cccaaggaaa gaggagctag cgatgcctcg
     1621 tccatccccc cagagattgg ccagagagca gatggcccgc cgggtcgccg ccgagctgtg
     1681 cgacgggttt tacgtcaacc tcggcatcgg catgccgacc ctggtcgcca actacatccc
     1741 cgacggcgtc gacgtcgtgc tgcagtccga gaacggcctg ctcggcatcg gcccgttccc
     1801 ctacgagggc gacgaggacc ccgacctgat caacgccggc aagcagaccg tcacgacgat
     1861 ccggggctcg gcgttcttcg actcggcgca gtcgttcgcg atgatccgcg gcggccacgt
     1921 cgacgtcagc gtgctcggcg cgatgcaggt ctccgcgcgc ggcgatctcg cgaactggat
     1981 gatccccggc aagatgatca aggggatggg cggcgcgatg gacctggtcg ccggcagccg
     2041 ccgcgtgatc gtgatgatgg agcacacggc caaggacggc gagcccaaga tcctgcgcga
     2101 ctgcacgctg ccgctgaccg gggtcgcgtg cgtccacacg atcgtcagcg acctcgccgt
     2161 gatcgacgtg accggcgaag gcctcgtgct gcgcgagcgc gcgcccggtg catccgccga
     2221 ggacgtcgcc aacgcgaccg gcgcgccgct gatctggacc gccagcgtcc ccgagatcaa
     2281 ggtctgaaga tgtgtagaaa attgagctcg cgctgagtcc gaggccgttg agcttgacct
     2341 tgacgttgtc gcagggcgtt gaccttgagg gcgatgtagt cgttgatcct gtcgtcgacc
     2401 ttggacgtga accccgggtc atttgcgaga aatcgtcctc gagatggagt caacgacgag
     2461 gtcaacctca gggtggggac ccagttcgaa cgctttgatc gacacatctg agcgccgcgc
     2521 gcaccgcact caccgcgctc agggcgtcgc caccgggcac ggcttgcaca cgtggccgtg
     2581 gcacgcggcc tcgtacggat tgaacttgtc gggcggctgg cagtcctcct tgggcggcaa
     2641 gcccgagcgc ttgctaccgc cgtcgcggct cgtggggcgg acggtcctgg agtcgtggtc
     2701 cgcactcgac gacttgccgg aggaccggcc gtcgcgttcg gcgccggtgg agccgcccgc
     2761 gctcgaggac gccccgccgc ccgacgcctt ggccgtgccc ggctcgggga gtggcgcgag
     2821 tgccacggtc tgctcgaagt cgcggccgag gtcgaccggc gcggtgacgt cctggtagcg
     2881 cgcgcggtgg atcgtcagcg gctcgctgcc ggcgcggcgc tcgccgtcgg cgcggcgccc
     2941 tcgccgggcg aagatcaagc ggccaagtcc ttcttcgagg acttgctgcg gcgaggccgg
     3001 ctcaagatcc ctcgcgccgg caagggagcg gccgaaaccg tacgcgcggc ggcgccgcag
     3061 acccacgaga cgcataccca cgaattgcgg cgggaaggaa aacacatggt gctgcgccgc
     3121 gtgcgtatcg actgcgggtt cgctcaccat tgagcgccgg ggttatgcgc ctgtatgatg
     3181 cgcggcggaa attcgggggc cacacatggc tgaccaatgc cgcgcatcaa ccaacaggcg
     3241 ctgaagacgg agcttgccaa gcgcggcttc gagtcgatct cgatcaaacg cgagttgccg
     3301 ggcggccgcg tcgaggtcga cgccaacaag ctccaccccg tgcatgacga aggcggcgaa
     3361 gccatttacg cgcccgtgcc ggtctcgtta tccgtcgagc ttgacgggcg cggcaaggtg
     3421 aagtcgatcg cgggcgacac tccgaaaccg gctgcagtcg ccgatgccgc ccgctacatg
     3481 aagacgctgc gcgacagcgg gcaactcgct atcgccggag ggggcacgcc ggcgcgcggg
     3541 gccacgcatc agatcgagcg cgatgcacaa ggacggcaag tcctgcggcg caagcgtttt
     3601 tcgataattt aatggcgt
//
LOCUS       DASVOR010000326         5293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382848,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000326 DASVOR010000000
VERSION     DASVOR010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5293
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382848"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF516_06745"
     CDS             <1..473
                     /locus_tag="VF516_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107410.1"
                     /translation="RRLVTASRRDAGTWGNLVSELGSIAELGRAVVSAIGDRLGGGAL
                     PAPSLALLASLARDALALDVPMVFLKQFRDAADGTAACYQAIVEAPNRMTRDPVEAGF
                     LHGEWELAIEQFPTVRLIDRLGLRARDGKLTALLHIWVKFGFTAELGQVIWRAV"
     gene            complement(483..1019)
                     /locus_tag="VF516_06750"
     CDS             complement(483..1019)
                     /locus_tag="VF516_06750"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEX8107411.1"
                     /translation="MRLLGICGSLRKASLNRALLAAVRDALPPDVQMTLHDALDLPIF
                     NNDLEDPPGVVRLKAAIAAADGVVFSVPEYNYSIPGGLKNALDWVSRPPDQSPLRGKP
                     IGMVGAASGMSGTIRAQTHLRQVMVYSDSPCLSQPEVLIPRAKERFDADGRLTDPSTR
                     VLLEKFGAAMAVFVRRFR"
     gene            1089..1754
                     /gene="msrA"
                     /locus_tag="VF516_06755"
     CDS             1089..1754
                     /gene="msrA"
                     /locus_tag="VF516_06755"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8107412.1"
                     /translation="MGMFFSSKKLRMPRPDEALPGRQTPMRVGDKHFVTGHRIVPPFP
                     DGMRRAMFGMGCFWGAERKFWQTRGVYATAVGYAGGLTPNPTYEEVCSGLTGHNEVVL
                     VIYDPREISYEQLLSVFWENHDPTQGMRQGNDSGTQYRSGIYAFDADQRTAAEASKAT
                     YAPRLAAAGYGPITTEIVDAPPFYYAEDYHQQYLGKNPDGYCGLGGTGVSCPVGLGAA
                     AAR"
     gene            1992..3377
                     /locus_tag="VF516_06760"
     CDS             1992..3377
                     /locus_tag="VF516_06760"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8107413.1"
                     /translation="MTERNPPERCRRRTQVQRYLFLVLEGARLEAGGLRVPLGDAAWL
                     QIGRGPARALVAGDDGAQVLEVPDPRMSGTHARIMRIAGTFTLQDAGSTNGTLVNGEP
                     IATHVLRDGDVLELGQTIFLYRELEAETLGRARSLDSRTAAVPAPGFATLDPDLALRL
                     ELLSRVAPSSLSILLLGETGTGKEVLAHGLHLLSRRPGPFVAVNCGAIPQNLVESQLF
                     GHVRGAFSGALRDEPGLVRSAQHGTLLLDEIGDLPLGSQAALLRVLQEGEVRPVGSTH
                     TVKVDVRIVAATHMPLAGLIERGAFRRDLYARLAGYTFLPAPLRERRMDLGLLVAALL
                     SAGKLGARRGIQLQGDAGRALVRHDWPMNVRELEQCLRAACMLSDDGVITAEDLRSAL
                     FAAGVAATPPPPGALACTVVPSFRVDQRARLVALLAEHHGNLSAVARAMQTSRTQVYR
                     LMERYKVSDGP"
     gene            3667..3984
                     /locus_tag="VF516_06765"
     CDS             3667..3984
                     /locus_tag="VF516_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107414.1"
                     /translation="MRPSTASDEKITDPLQAQLSCGPIVVYMNGFAHGVHGGFGSFCP
                     DTPVARSILHDNHKTGYLPGFCSTCLGVPQGWIFVIWQETKGPLCPDGCAPGTVGQSA
                     PLL"
     gene            complement(4004..>5293)
                     /locus_tag="VF516_06770"
     CDS             complement(4004..>5293)
                     /locus_tag="VF516_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107415.1"
                     /translation="FGTMFPRTRPDAAFGQYETNAVELARRDFAQMMSSATGTRARPF
                     GVIACDDAAAFHRAARHLVDVGVPAVIGFFKTPEAIELTTSVFLPRRMLTIASLNTNP
                     LVTRVPHPDGVPRLVWRTTYNNTSAAAALSAWIRSDVEPAMRARGEHGRRDMRVALLR
                     QSGLAGDALSAAFLSTLRFTGKAAVDNANFRELTIEAAAPGTDPGYGRMLDELLRLQP
                     DVILYAASVPIIEAVFAPLEARFPRGAPRPRYASIAVFPPELLAFIGTDPERRSRFFA
                     VTPVGSTEANARFVTHFNETFSDSDPITRTYSPNASYDAFYLLAYATYAIPSHEVVTG
                     ERLARAFGKLLPPGRPIDVGLAGIYDAYDALSKGASIDLTGATGSLDFDPSTGEYALD
                     QAILCVGVDPHGRPEGIESGRVYSARTNALVGTLHCP"
BASE COUNT          738 a         1849 c         1915 g          791 t
ORIGIN      
        1 ggcgccggct ggtcaccgcg tcgcggcgcg atgccggcac gtggggcaac ctggtcagcg
       61 agctgggctc gatcgccgag ctcggccgcg ccgtggtgtc ggcgatcggg gatcggctcg
      121 gcggcggcgc gctgcccgcc cccagcctcg cgctgctggc aagcctcgcc cgcgacgcgc
      181 tcgcgctcga cgtgccgatg gtgttcctca agcagttccg cgacgccgcc gacggcacgg
      241 ccgcgtgcta ccaggcgatc gtcgaggcgc ccaaccggat gacccgcgat ccggtcgagg
      301 ccggcttcct gcacggcgag tgggagctcg cgatcgagca gttcccgacc gtgcggctga
      361 tcgaccggct cgggctgcga gcgcgcgacg gcaagctgac ggccctgctc catatctggg
      421 tcaagttcgg gttcaccgcc gagctcggcc aggtgatctg gcgcgccgtg tgacccgcgc
      481 gatcagcgga accggcggac gaacacggcc atcgcggcgc cgaacttctc gagcagcacg
      541 cgggtcgacg gatcggtcag ccggccgtcg gcatcgaacc gctccttggc gcgcgggatc
      601 agcacctcgg gctggctcag gcacggcgag tcgctgtaga ccatgacctg gcgcaggtgg
      661 gtctgcgcgc ggatcgtgcc gctcatcccc gaggcggcgc cgaccatgcc gatcggcttg
      721 ccgcgcagcg gcgactgatc cggcggccgc gacacccagt cgagcgcgtt cttgagcccg
      781 cccgggatgc tgtagttgta ctccggcacg ctgaacacca cgccatcggc cgcggcgatc
      841 gccgccttga gccggaccac tcccggcgga tcctcgagat cgttgttgaa gatcggcaga
      901 tcgagcgcgt cgtgcagcgt catctggacg tccggaggca gcgcatcgcg tacggctgcg
      961 agcaatgcgc ggttgagcga cgccttgcgc aggctaccgc agatccccag gagcctcatc
     1021 tcggcgaacc tagcactgtt accaggtgtc ggagccgtta ctttcgccgc cgtcgccgta
     1081 tggtacgcat gggtatgttc ttcagctcga agaagctccg catgccgagg ccggacgagg
     1141 cgctgccggg ccggcagacg ccgatgcggg tcggcgacaa gcacttcgtg accggccacc
     1201 ggatcgtgcc gccgtttccc gacggaatgc ggcgcgcgat gttcggcatg ggctgcttct
     1261 ggggcgccga gcgcaagttc tggcagaccc gcggggtgta cgcgaccgcc gtcggctacg
     1321 ccggtgggct gacgccgaac ccgacctacg aggaggtctg ctcggggctg accgggcaca
     1381 acgaggtcgt cctggtgatc tacgacccgc gcgagatcag ctacgagcag ctgctctccg
     1441 tgttctggga gaaccacgat ccgacgcagg gcatgcggca gggcaacgac tccggcacgc
     1501 agtatcgcag cgggatctac gcgttcgacg ccgaccagcg caccgcggcc gaggcgtcga
     1561 aggcgaccta cgcgccgcgc ctggcggcgg ccggctacgg cccgatcacc accgagatcg
     1621 tcgacgcgcc gccgttctac tacgccgagg actaccacca gcagtacctc ggcaagaacc
     1681 ccgatggcta ctgcggtctc ggcggcaccg gcgtgtcgtg cccggtcggc ctgggcgcgg
     1741 ccgcggcccg gtagtcggcg tcctgttcga agttcgattc ggggtgtgcg atcgacgtcg
     1801 tgcacctcgc gcgtcacgaa gcgcgcttcg ccttgccgcg aaatgcggcg tcatggtgcg
     1861 aagctcgctt cggtggccgg cgcgactccg catcgcgccc actgcgtgat atgctggcgc
     1921 gacaaggatg ggggaggggt gagatggagc gggatggcgt ggggtgtgct gtgagttggg
     1981 tggggagcca ggtgaccgag cgaaatccgc cggagcgatg ccgccgcagg acacaggtcc
     2041 agcgctacct gtttctcgtc ctcgagggcg cgcggctcga ggccggtggg ctgcgcgtcc
     2101 cgctcggcga cgcggcgtgg ctccagatcg gccgcggccc ggcgcgggcg ctggtcgcag
     2161 gggacgacgg ggcgcaggtg ctcgaggtcc ccgatccgcg gatgtccgga acccacgcgc
     2221 ggatcatgcg gatcgccggc acgttcacgc tccaggacgc gggctcgacc aacggcaccc
     2281 tggtcaacgg cgagccgatc gccacgcacg tgctgcgcga cggcgacgtc ctcgagctcg
     2341 gccagacgat ctttctctac cgcgagctcg aggcggagac cctgggccgg gcccgcagcc
     2401 tggactcgcg caccgcggcc gtcccggctc ccgggttcgc gacgctcgat cccgacctcg
     2461 cgctccggct cgagctgctg tcgcgcgtgg cgccgtcgtc gctgtcgatc ctgctgctcg
     2521 gcgagaccgg caccggcaag gaggtcctcg cgcacgggct gcacctgctg tcgcgccgcc
     2581 ccggtccgtt cgtcgcggtc aactgcggcg ccatcccgca gaacctggtc gagagccagc
     2641 tgttcggcca cgtccgcggg gcgttctcgg gggcgctgcg ggacgagccc gggctcgtcc
     2701 gctcggcgca gcacgggacg ctgctgctcg acgagatcgg cgacctgccg ctcggctcac
     2761 aggcagcgct gctccgcgtg ctccaggagg gcgaggtgcg gccggtcggc agcacgcaca
     2821 ccgtgaaggt cgacgtccgg atcgtcgcgg cgacgcacat gccgctggcc ggcctcatcg
     2881 agcgcggggc gtttcgccgc gatctgtacg cgcgcctcgc cggctacacg ttcctgcccg
     2941 cgccgctgcg cgagcgccgg atggacctcg gcctgctggt cgccgcgctg ctgtccgccg
     3001 gcaagctcgg cgcacggcgc ggcatccagc tgcagggcga cgccgggcgt gcgctggttc
     3061 gccatgactg gccgatgaac gtgcgcgagc tcgagcagtg cctgcgcgcc gcctgcatgc
     3121 tcagcgacga cggcgtgatc accgccgagg acctgcggtc cgctctgttc gcggccggcg
     3181 ttgcggcgac cccgccgccc ccgggcgcgc tcgcttgcac cgtcgtgccg tcgttccggg
     3241 tcgatcagcg cgcgcggctg gtcgcgctgc tcgccgagca tcacggcaac ctgagcgcgg
     3301 tcgcccgggc gatgcagacc tcgcgcacgc aggtctatcg cctgatggag cgttacaagg
     3361 tcagtgatgg accgtaggcg gtcgcccggc cgatcgcacc ggttcgatcg tacccggtac
     3421 acgccggtac acggccggtg cacgccgccc tcgataaccc acggaatgcg cgatcgcgcg
     3481 ccgtggtgca ccagatgcta gtgcggaagc ccggacagag aggagagcgc gatgaggtgg
     3541 agagcgatga ggtggacatc aggacttcga ttcgttgcca gtgcgttgct gtgggtcgcg
     3601 tgcgggccgc tgccgccgcc ggcgcccccg tgccagggga gctgtgtggc gcagcccggt
     3661 tgctcgatgc gaccgagcac cgccagtgat gaaaagatca ccgacccgct gcaggcccag
     3721 ctgagctgcg ggccgatcgt cgtgtacatg aacggcttcg cccacggcgt gcacggtggc
     3781 ttcgggtcgt tctgcccgga cacgccggtc gcccgaagca ttcttcacga caaccacaag
     3841 accggctacc tgccgggctt ctgctcgacc tgcctcggcg tgccgcaggg ctggatcttc
     3901 gtgatctggc aggagaccaa gggcccgctc tgccccgacg gctgcgcccc cggaacggtc
     3961 ggacaatcgg ctccgctgct gtgagtgcgg gcgcagctcg cactcacggg cagtgcaggg
     4021 tgccgaccag cgcgttcgtg cgcgcggagt agacccggcc ggactcgatc ccctccggtc
     4081 ggccgtgcgg gtcgacgccg acgcacagga tcgcctggtc gagcgcgtac tcgccggtcg
     4141 atggatcgaa gtcgaggctg ccggtcgcgc cggtcaggtc gatcgacgcg cccttgctga
     4201 gcgcgtcgta cgcgtcgtag atcccggcaa gtccgacgtc gatcggacgc ccgggcggca
     4261 gcagcttgcc gaacgcacgc gcgaggcgct cgccggtcac gacctcgtgg ctcgggatcg
     4321 catacgtcgc gtaggcgagc aggtagaacg catcgtacga cgcgttcggg ctgtacgtgc
     4381 gcgtgatcgg gtcggagtcg gagaacgtct cgttgaagtg cgtgacgaac cgggcgttcg
     4441 cctcggtcga tcccaccggg gtgaccgcga agaaccgact ccgtcgctcc gggtcggtcc
     4501 cgatgaacgc gagcagctcc ggcgggaaca ccgcgatgct ggcgtagcgc ggccgtggcg
     4561 cgccgcgcgg gaagcgcgcc tccagcggag cgaacaccgc ctcgatgatc gggacgctgg
     4621 ccgcgtagag gatgacgtcg ggctgcaggc gcaggagttc gtcgagcatc cggccgtatc
     4681 ccgggtcggt cccgggcgcc gcggcctcga tcgtgagctc gcggaagttc gcgttgtcga
     4741 ccgcggcctt gccggtgaac cgcagcgtgc tcaggaacgc ggcgctcagc gcatcgccgg
     4801 ccagaccgct ctgacgcagc agcgcgacgc gcatgtcccg gcgtccgtgc tcaccgcggg
     4861 cgcgcatcgc cggctcgaca tcgctgcgga tccacgccga cagcgccgcg gccgcgctgg
     4921 tgttgttgta cgtcgtgcgc cacaccaggc gtggcacgcc gtcggggtgc ggcacgcgcg
     4981 tgaccagcgg gttcgtgttg agcgacgcga tcgtcagcat ccgcctgggc agaaacaccg
     5041 aggtcgtcag ctcgatcgcc tccggggtct tgaagaaccc gatcaccgcc ggcacgccga
     5101 cgtccaccag gtggcgcgcc gcgcggtgga acgcggcggc atcatcgcac gcgatcactc
     5161 cgaacggtcg cgcgcgcgtg cccgtcgcgc tgctcatcat ctgtgcgaag tcgcgccgcg
     5221 cgagctcgac cgcgttggtc tcgtactggc cgaacgccgc gtcgggtcgg gtccgcggga
     5281 acatcgtgcc gaa
//
LOCUS       DASVOR010000327         4148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_149014,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000327 DASVOR010000000
VERSION     DASVOR010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4148
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149014"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1711)
                     /locus_tag="VF516_06775"
     CDS             complement(308..1711)
                     /locus_tag="VF516_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107416.1"
                     /translation="MKTGVRSTVGWVAWAAALCIVVHAGCDRRSSALRLGVARSNLGA
                     LSGESQMATWTCTGDPCPWGDELSNPALAWPAEAQPVTTRLGYTVSPGVYLPASSANG
                     LTVTIDSGSVRVYKGQPQESSHHRLALLEEGDSFDVSGLDPDEVLSVQSDDPFTYHVA
                     PTEPGDPGDQPDAGTDDAATPDAAAPDAATPDAPPPPDGPASASQAITWTCTGRQCPF
                     GPTLTGQALVWQVERGAIHTHLGYTTSDTIYLFGSRANGVDLWIDTGTANLLAGLPGD
                     IWPRTLATLHAGDTFHVTGLSVSEVLSAQSDQPFTYRVSVPPASDPPGDPSSGGPGDL
                     MQAPPALWRCNVPECWSDDWTGAVVPWPSWAAYTSNARPGNLSRSVFAWDDTPLYPYM
                     GAWADGCEITGESGIARVIEWQRGSDHWRDTWLYPGQTHVIHLVPPEDGALIEGYDAS
                     PGFSVTVRNCTPQPLQP"
     gene            complement(1862..>4148)
                     /locus_tag="VF516_06780"
     CDS             complement(1862..>4148)
                     /locus_tag="VF516_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8107417.1"
                     /translation="APPEPAPPEPAPPEPAPDESVPISEVIVVTSTTPLHGSHLPKDH
                     VPANVQTISAEDLADHKSLDLSTYAGEAIGSVHLNDVQGNPLQSDVQYRGFLASPLLG
                     APQGVSMYLDGVRLNEPFGDTINWDLIPSNAIRSVNVIPGSNPIFGLNTLGGALSLET
                     KTGFSDPGLDGTLLYGSWGRKLARVNAGVHGERFGIFAAAQVFDEDGWRDLAPTRSEH
                     GFVSASYQHAGTTADLSLIGANTRLTGNGPLPEQQLAMDRTVFFTAPDITENRMFMAA
                     LRGEHPLSAHLRLSGMVYGRTNRTRTVNGDQHDWAACMATPGALCSTDDQGNETPVLD
                     SQGMPVAFSDSYDAANNRTDTRQTSYGAAAQLAVDAPLAARENHLFVGVDASQSRIRF
                     RSQTTVGTLDADRVVSDAGFVDPASPIAVDSTVDNLGVYATDTFSIRPDLFVTLSGRF
                     NLTRLSLDDRLGDALSGDHAFHRINPAAGISYQPRRWLGGYASYSESNRAPTAVELTC
                     ASPTDPCRLPNAFLSDPPLAQVVARTVELGLRGTARPSGVTLDYDLAAFHTVNSSDIL
                     FISSGMVANQGYFANVGDTRRQGIEADLTARKRLDGGLRIDGSVHYTFLAATFQTPFT
                     APSALHPAAVNGLIDVPAGAHIPSVPRHIVKLALSGSAFGWSGGVSVVGNTGQYLRGD
                     EANLLAPVSGYVVVNARVAYRVGAHASVFVLASNLFDAGYSTFGVLGDATGVLGPTYD
                     SPRFLGVGAPRAAWLGLDLND"
BASE COUNT          594 a         1411 c         1520 g          623 t
ORIGIN      
        1 ctcgttggcc ggcacccagc gccaggcgtc gaagccgcgc cgcgcggcgg ccagcgcgtc
       61 gtccaggtcg gcggtcgacg cgtgcgccac gcgaccgatg cgctagccgg tggccgggtt
      121 cagcacgtcg agcgtcttgc cgcccgcggc gtcgcgccac tggttgtcga tcagcaggcg
      181 ggtatcggga tacggagagt cagggggcat gggcaacctc gggtcgagat gtcggcgcgg
      241 cgcggtgcga cggatcaccg ccgcggcatc ttatgccggg ttgccgtccg gctgaacgtg
      301 cgccgcgtca gggttgcagc ggctgcggcg tgcagttgcg cacggtcacg ctgaatcccg
      361 gtgacgcgtc gtagccttcg atcagcgccc cgtcctccgg cgggaccagg tggatgacgt
      421 gggtctgtcc cggatacagc caggtatccc gccagtggtc ggacccgcgc tgccactcga
      481 tgacccgcgc gatgccggac tcgccggtga tctcgcagcc gtcggcccag gcgcccatgt
      541 acggatacag cggtgtatcg tcccacgcga acaccgagcg cgacaggttg ccgggccggg
      601 cattgctcgt gtacgccgcc caggacggcc acgggacgac cgcgcccgtc cagtcgtcgc
      661 tccagcactc ggggacattg cagcgccaca gcgccggggg cgcctgcatc aggtcgccgg
      721 ggccgccgga ggatggatcg cccggggggt cgctcgccgg cggcacgctg acgcgatagg
      781 tgaacggctg gtcggattgc gcgctcagga cctcactgac gctcagcccg gtgacatgaa
      841 acgtatctcc ggcgtgcagg gtcgccaggg tgcggggcca gatgtcgccc gggagtcccg
      901 ccagcaggtt cgccgtgccg gtgtcgatcc agaggtccac gccgttcgcc cggctcccga
      961 acaggtagat ggtgtccgag gtggtgtagc cgaggtgcgt gtggatcgcg cctcgctcga
     1021 cctgccagac cagcgcctgg ccggtcagcg tcgggccgaa cgggcactgg cggccggtgc
     1081 aggtccacgt gatcgcctgc gatgcgctgg cgggaccgtc gggcggcggg ggtgcatcgg
     1141 gcgtcgcagc atcgggcgcc gcggcatccg gtgtcgctgc atcgtccgtc ccggcatcgg
     1201 gctggtcacc cgggtcgccc ggttccgtcg gcgccacgtg ataggtgaac gggtcatccg
     1261 actgcacgct cagcacctcg tcggggtcca gaccggacac gtcgaagctg tcgccttcct
     1321 cgaggagcgc cagccggtgg tgcgaggact cctgcggttg gcccttgtac acgcggacgc
     1381 tgccggagtc gatcgtcacg gtcaggccgt tggcgctgct cgccgggaga tagacccccg
     1441 gtgacaccgt gtagcccagc cgcgtggtga ccggctgggc ctctgccggc caggcgagcg
     1501 cgggattgct cagctcgtcg ccccacgggc acgggtctcc cgtgcaggtc caggtcgcca
     1561 tctgcgattc cccggacagc gcgccgaggt tgctccgggc cacgccgagg cgcagcgcgc
     1621 tcgaccgccg atcgcagccg gcgtgtacca cgatgcagag tgccgccgcc cacgccaccc
     1681 agccgaccgt cgatcgtact cctgttttca cgtgtacctc cgtatcggtt ctgcaggtca
     1741 atgcgttcga ccatcgtgcc tgctccgggc cggacccgga tccggcgaag gccgggctca
     1801 cgatcgatcg ccggctgacg accatgtgcg gatcaccgcg acacgcgtcc ttgcgcggca
     1861 ctcagtcgtt caggtcgagg cccagccacg ccgcgcgcgg ggcgccgacg cccaggaacc
     1921 gcgggctgtc gtacgtcggg ccgagcacgc ccgtggcatc cccgagcacg ccgaacgtcg
     1981 agtacccggc gtcgaacagg ttgctggcca ggacgaagac cgacgcgtgc gcccccacgc
     2041 ggtacgcgac ccgcgcgttg acgaccacat acccggagac cggcgccagc aggttcgcct
     2101 cgtcgccgcg caggtactgg ccggtgttgc cgacgacgct caccccgccg gaccacccga
     2161 aggcggaccc gctcaacgcc agcttgacga tgtggcgcgg gaccgacggg atgtgcgcgc
     2221 cggccgggac gtcgatgagc ccgttgaccg cggcggggtg gagcgcgctc ggcgccgtga
     2281 acggcgtctg gaaggtcgcg gcgaggaacg tgtagtgcac ggacccgtcg atgcgcagac
     2341 cgccgtcgag ccgcttgcgg gcggtcagat cggcctcgat cccctggcgc cgggtatcgc
     2401 ccacgttcgc gaagtagccc tggttggcca ccatccccga gctgatgaag aggatgtcgc
     2461 tggagttgac cgtgtggaag gccgcgaggt cgtagtccag ggtgacgccg gacggccgcg
     2521 ccgtgccacg caggccgagc tcgacggtgc gcgcgacgac ctgggcgagc ggcggatcgc
     2581 tcaggaaggc gttcggcagc cggcacggat cggtcgggct cgcgcaggtc agctcgacgg
     2641 ccgtcggcgc ccggttggat tcgctgtagc tggcgtagcc gccgagccag cggcggggct
     2701 gatagctgat cccggcggcc ggattgatcc gatggaacgc gtgatcgccg gacagcgcgt
     2761 cgccgagccg gtcgtcgagg gacagccggg tcaggttgaa tcgccccgac agcgtgacga
     2821 acagatcggg gcggatcgag aacgtatcgg tcgcatagac accgaggtta tcgacggtgc
     2881 tgtcgaccgc gatcggggac gccggatcca cgaagccggc gtcgctcacc acgcgatcgg
     2941 cgtcgagcgt cccgaccgtc gtctgcgagc ggaaccggat ccggctctgg ctcgcatcga
     3001 caccgacgaa caggtggttc tcgcgcgcgg cgagcggtgc gtcgacggcg agctgcgcgg
     3061 cggcgccgta gctggtctgc cgggtgtccg tccggttgtt cgcggcatcg taggagtcgc
     3121 tgaacgcgac cggcatcccc tggctgtcga ggaccggcgt ctcgttgccc tggtcgtcgg
     3181 tcgagcacag cgcgccgggc gttgccatgc acgccgccca gtcgtgctga tcgccgttga
     3241 ccgtccgggt ccggttcgtc ctcccgtaga ccatgccgga cagccggagg tgggccgaca
     3301 gcggatgctc tccgcgcagg gccgccatga acatccggtt ctcggtgata tccggtgcgg
     3361 tgaagaacac ggtgcggtcc atggcgagct gctgctcggg caatggcccg ttccccgtca
     3421 agcgggtatt ggcaccgatc agcgagagat cggccgtcgt gcccgcgtgc tggtacgacg
     3481 cggagacgaa gccgtgctcc gaccgcgtcg gcgccaggtc gcgccagccg tcctcgtcga
     3541 acacctgcgc cgcggcgaag atcccgaacc gctcgccgtg cacgccggcg ttcacgcggg
     3601 ccagcttgcg gccccacgag ccgtacagca gcgtcccatc cagccccgga tcggagaatc
     3661 cggtcttggt ctccagcgac agcgccccac ccagcgtgtt gagaccgaag atcgggttgg
     3721 acccgggaat cacgttgacc gatcggatcg cgttcgaggg gatgagatcc cagttgatcg
     3781 tgtcaccgaa cggctcgttc aggcggacgc catcgaggta catcgacacg ccctggggag
     3841 cgccgagcag cggagacgcg aggaagccgc ggtactggac gtccgactgc agcgggttgc
     3901 cttgcacgtc gttgaggtgg acgctgccga tggcctcgcc cgcgtacgtc gacaggtcga
     3961 ggctcttgtg atcggcgaga tcctcggccg agatggtctg gacgttcgcc gggacgtgat
     4021 ccttgggaag gtgcgagcca tgcagcggcg tcgtcgacgt gacgacgatg acctcggaga
     4081 tcgggaccga ctcgtccggg gccggctccg gcggggccgg ctccggcggg gccggctccg
     4141 gcggggcc
//
LOCUS       DASVOR010000328         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265841,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000328 DASVOR010000000
VERSION     DASVOR010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265841"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VF516_06785"
     CDS             complement(<1..510)
                     /locus_tag="VF516_06785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX8107418.1"
                     /translation="MNPIVPRDLDIDFRPVPRHWLAHNATVTAIANGVNLLFPHGERF
                     FVRSVKHFLDQIDDPELRAAIKGFFGQEGRHARAHDQFNAILRAQGYEIDRFLTGYQR
                     LSSWLEAHNPPKLNLAITAAAEHFTAILAEGAFTENVLDAAAPEMRQLLAWHAAEELE
                     HKAVAFDVLR"
     gene            633..1358
                     /locus_tag="VF516_06790"
     CDS             633..1358
                     /locus_tag="VF516_06790"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8107419.1"
                     /translation="MPPGRAVRSSRGAARPRRRIRLDNDQRRAQLLELAKRAFSDRSY
                     DEVSIDDLARGAGISKGLLYHYFPTKRHLYVAGLREIADDLVDAVLRVATDLPLADPT
                     GGSAPRPVGDAVAAPTLRLALIDRVRASVDAYLEHIARHSRAFVALMRGGIGSDPEVA
                     AVVDSVRTRLFDKLLSGSALASLVTSDARLETAMRGWIGFVEAASIDWCANPRLSQGE
                     LRDLMTAVLLEVIRVAAPPVTAR"
     gene            complement(1380..>2385)
                     /locus_tag="VF516_06795"
     CDS             complement(1380..>2385)
                     /locus_tag="VF516_06795"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8107420.1"
                     /translation="GAPWPTVFQTVQIVAAADASLAQVYGFQHVLLATCQLFGTPEQA
                     RRLLEATAAQRWFWGNALNPLDRRLALVATPSGPVLRGDKSFSTGAIDSDMLMVSALE
                     DGTGKLIVAAIPTARAGIELRDDWDNMGQRQTDSGSARFHDVAVSGDEILAAPGGPLG
                     STLATLRACIAQLAFTHIFLGIADAALAAARDYVRTRTRPWYLTKLESNTQDPYVLAH
                     FGEMVAALAGAQAVADRAAQALERAWQRGDALTVDERGGVAVEIATAKLLASRAVLDI
                     TSQIFETTGPGATSTAWGFDRFWRNARTLTLHDRLDYKVHDLGQWYLNEQWPPPSFYS
                     "
BASE COUNT          327 a          823 c          874 g          361 t
ORIGIN      
        1 gcgcagcacg tcgaacgcga ccgccttgtg ctcgagctcc tcggcggcgt gccacgccag
       61 cagctgccgc atctcgggcg ccgcggcgtc gagcacgttc tcggtgaacg caccctcggc
      121 gaggatcgcc gtgaagtgct cggccgccgc ggtgatcgcg aggttgagct tgggcgggtt
      181 gtgggcctcc agccacgagc tcagccgctg gtagccggtg aggaagcggt cgatctcgta
      241 gccctgggcg cgcaggatcg cgttgaactg gtcgtgggcg cgggcgtggc ggccctcctg
      301 gccgaagaag cccttgatcg ccgcgcgcag ctccgggtcg tcgatctgat cgaggaagtg
      361 cttgaccgag cgcacgaaga accgctcgcc atgcggaaac agcaagttga cgccattggc
      421 gatcgccgtc accgtggcgt tgtgggcgag ccagtgtcgc ggcacgggcc ggaaatcgat
      481 atcgagatcg cggggaacga tcgggttcac gcggagcctc caggagatgg tgcgctcatc
      541 gtaaagccac tattgaccaa aagtcaatag tgacctgggt tcgcgggacg gccggcgggc
      601 tgccgcgcgc gattgccgtt atgatgctcg cagtgcctcc cggtcgggcc gttcgcagtt
      661 ctcgtggcgc cgctcgcccg cggcggcgga tccgcctgga caacgatcag cgccgggccc
      721 agctcctcga gctcgccaag cgcgcattct cggatcgctc gtacgacgag gtctcgatcg
      781 acgacctcgc gcggggcgcg ggcatctcca agggcctact ctaccactac ttcccgacca
      841 agcggcatct ctacgtcgcg gggctgcgcg agatcgccga cgatctggtc gacgcggtgc
      901 tgcgcgtcgc cacggacttg ccgctggccg atcccacggg gggctctgct ccccgccccg
      961 tcggcgacgc cgtcgcggct cccaccctcc gtctcgcttt gatcgaccgg gtacgggcga
     1021 gcgtcgacgc ctatctggag cacatcgcgc gccactcgcg cgcgttcgtc gcgctgatgc
     1081 gcggtgggat cggcagcgac cccgaggtcg cggcggtggt cgacagcgtg cggacgcggc
     1141 tgttcgacaa gctgctgtcg ggctccgcgc tcgccagcct ggtcaccagc gacgcgcggc
     1201 tggagaccgc gatgcgcggc tggatcggct tcgtcgaggc cgcgtcgatc gactggtgcg
     1261 ccaacccgcg gttgtcgcaa ggcgagctgc gcgacctgat gaccgcggtg ctgctcgagg
     1321 tcatccgggt cgcggcgccg ccggtcaccg cgcggtgacc tccacccggc gcccgggcat
     1381 cacgagtaga aggacggggg cggccactgc tcgttgaggt accactggcc gagatcgtgg
     1441 accttgtagt cgagtcgatc gtgcagcgtc agggtgcgcg cgttgcgcca gaaccggtcg
     1501 aagccccacg cggtgctggt cgcgccgggc ccggtggtct cgaagatctg cgaggtgatg
     1561 tcgaggacgg cgcggctcgc gagcagcttg gcggtggcga tctcgaccgc gacgccgccg
     1621 cgctcgtcga ccgtcagcgc gtcgccgcgc tgccacgcgc gttcgagggc ctgcgccgca
     1681 cggtcggcga cggcctgcgc gcccgcgagc gccgcgacca tctcgccgaa gtgcgcgagc
     1741 acgtagggat cctgggtgtt ggactcgagc ttggtcaggt accacggccg ggtccgggtc
     1801 cgcacgtagt cccgggccgc agccagcgcg gcgtcggcga tcccgaggaa gatgtgggtg
     1861 aacgccagct gcgcgatgca cgcccgcagg gtcgcgagcg tgctgccgag cggccccccc
     1921 ggcgctgcga ggatctcgtc gcccgagacc gccacgtcgt ggaaccgcgc gctgccgctg
     1981 tcggtctggc gctggcccat gttgtcccag tcgtcgcgga gctcgatgcc ggcgcgcgcg
     2041 gtcgggatcg ccgccacgat cagcttcccg gtgccgtcct cgagcgccga gaccatcagc
     2101 atgtcggagt cgatcgcgcc ggtcgagaac gacttgtcgc cgcgcagcac cggtccggac
     2161 ggcgtcgcga ccagcgcgag ccggcggtcc agcgggttca gcgcgttgcc ccagaaccag
     2221 cgctgcgccg cggtggcctc cagcagccgg cgcgcctgct cgggcgtccc gaacagctga
     2281 caggtggcga gcaggacgtg ctggaagccg tagacctggg cgagcgacgc gtcggccgcg
     2341 gcgacgatct ggacggtctg gaacacggtc ggccacggcg caccg
//
LOCUS       DASVOR010000329         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_499942,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000329 DASVOR010000000
VERSION     DASVOR010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499942"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VF516_06800"
     CDS             <1..479
                     /locus_tag="VF516_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107421.1"
                     /translation="IAIAALLRDDDDRVRVAAAQALDQLGDDAVVAAAPYVVSALRGD
                     ARLAEVCRAVLVARKSRVEAALIAGLETSDQVHGMRIADVICALPNARELLFAAFDGE
                     AQNVQINAAFGIAQLGVKRAGREGRQRLLNGLPGPPTRRRDAMVKALAMLGESPAR"
     gene            complement(775..1824)
                     /locus_tag="VF516_06805"
     CDS             complement(775..1824)
                     /locus_tag="VF516_06805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107422.1"
                     /translation="MTNITEAPVESAPALRTWLRDFVYEARGALSEQLLPVVRWLAEK
                     QRLRCILGAVCADSELVQRAGAQSYIHINGFDKLVLLSEPDVFKLRLHIWWGDNAALS
                     KETIHNHRWDFAAHIVTGAYRAEEFERADAGAPFREYRYYSPEADGQFGYEECGPARL
                     RTASDRLVAAGTGYFLSHEVLHRVSQDMPTLTASLVLQGPTMKRYTNSLLDRPVRLDG
                     HAVPSPRRLSGAEMADRCGRFLNRLDGKNDAPERAPQARMSLARDVLRGYAGQYRHEG
                     SHPFALEIEAVEEHLRVCADLLPRPFDLYPSSPVDFFFDNIDVRLHFVMGQGGAVQGV
                     NVRFPSFESFAKRVG"
     gene            complement(1895..2917)
                     /locus_tag="VF516_06810"
     CDS             complement(1895..2917)
                     /locus_tag="VF516_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107423.1"
                     /translation="MTSPSPPPTAAGLKERLERLAHGTPIDVAAILRHAPETVSPEAA
                     RRAQALLGKFLRASDRDAVGIVALNQCWEWTAAAAPSERPSDPEANLRTHGLRLAAPV
                     AAVDIYLAEPGGLTSPDSHALRLTHAHGATFLLPISKRDTGERALLETDGGTLAARGE
                     EVMLVYSFAHPPVVAAFLEAMSRSSERVSSVIFKRTRSIEPVCLPKTLSSHAPAVSSQ
                     EDQPLAFLVATTPFVQGSFEEGRWLRPVRQPPLAAFAAAIGRALLAREPVSSAALEQA
                     LGESSCRLFRCVEVEWRGETARAESTRLASAVHAAFPDLETTLARKNDPEFPAGPCHE
                     ALPANP"
     gene            complement(2914..4053)
                     /locus_tag="VF516_06815"
     CDS             complement(2914..4053)
                     /locus_tag="VF516_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107424.1"
                     /translation="MKLKDTGRIVLSTVRTIAPDDLMAPDDLVEAAGLPGIRLYRRDD
                     YALLLDWSRILPAFYDGHEDGAPALLDQLIGHEQGDPLGLHTTHKLILVCEGVGRPTG
                     ALCLNHKRGGSTKLGPLLIDRGAPHKRVVATRLFEAALTIARLTGRRKLYGTFPRRNA
                     GMAAVLLGVAPEAGLKEEAAFPDHFRTGVTETVWGMHLDKPPLAVASVSSSLESGQGQ
                     AFRIGLYEDEDWGYFQSAYKVCRQWHDDADDELPRAQVAAHWRGLAGGDHLYQRKSTR
                     ILVARGHDGHRSGLLILTPKRGGPAKLYPLIGTALAQEELLQTAIAEVAALGGRKLYT
                     FAHERDERQLAFLEAQGFVRRGLLESPYRPGHNLVPMDRPLETRP"
BASE COUNT          745 a         1414 c         1482 g          702 t
ORIGIN      
        1 cgatcgcgat cgcggcgctg ctccgcgacg acgacgaccg cgtccgggtc gcggcagcgc
       61 aggcgctcga ccagctcggc gacgacgcgg tggtcgccgc cgcgccgtac gtggtcagcg
      121 ccctccgcgg cgatgctcgg ctggccgagg tctgccgcgc ggtgctcgtc gcccgcaaga
      181 gccgggtcga agccgcgctg atcgccgggc tcgagacctc ggaccaggtc cacggcatgc
      241 ggatcgccga cgtgatctgc gcgctgccga acgcgcgcga gctgctgttc gccgcgttcg
      301 acggcgaggc gcagaacgtc cagatcaacg cggcgttcgg catcgcccag ctgggcgtca
      361 agcgcgccgg ccgggaggga cgccagcgct tgctgaacgg cctcccgggc ccacccacgc
      421 gccggcgcga cgcgatggtc aaggcgctgg cgatgctggg cgaatcgccg gctcgctaga
      481 gggcgaaaca tgtactgacg cgatgacaga acgctggagc tgcatatgcc tgtgagcgcg
      541 caccacgcgc gagtccgtct ctcgcccctc cggagacggc ggcggcgacg ggggacagaa
      601 gctcccacag gagcggcgcg cggacaccct tcaagcgacg caacgtcccg ccggcgagat
      661 tcatgagctg gaaccgcagg ggcaatcggg ctcgctccag ggtcagatcg tgagaagcca
      721 tgacattctc ccgaggttcg cgcggtagcg gcgggcgctg gaagcaggcg tcggctagcc
      781 cacgcgcttc gcgaagctct cgaatgatgg gaatcgaaca ttgactccct gaaccgctcc
      841 tccttggccc atcacgaaat ggagacgaac atcgatgttg tcgaagaaga aatcgaccgg
      901 agagctcgga tacaggtcga acggcctcgg caacaggtcc gcgcagacgc gcagatgctc
      961 ctccacagct tcgatctcca gcgcaaacgg atgactcccc tcgtgccggt attgcccggc
     1021 gtaaccgcgc aggacgtcgc gtgccagcga catccgcgct tgcggcgcac gctcgggcgc
     1081 atcgttcttt ccatccaggc ggttcaggaa gcggccacac cgatctgcca tctccgcgcc
     1141 cgatagtcgc cgcggcgaag gcacggcatg gccgtcgagc cggactggcc ggtccaggag
     1201 agaattcgtg taccgcttca tcgtgggccc ctgcaggacc aacgaggccg tcaaagtggg
     1261 catgtcctga ctgactcgat gcaggacctc gtggctcaga aaatagccag tcccggcggc
     1321 cacgagtcga tcggaggcgg tccgcaacct cgccggcccg cattcttcgt acccaaactg
     1381 cccgtctgcc tcgggcgagt agtaccggta ctctcgaaac ggcgccccag cgtcggcgcg
     1441 ttcgaactcc tccgccctgt atgccccagt gacgatgtgt gccgcaaaat cccagcggtg
     1501 attgtgaatt gtctccttcg aaagggcggc attgtcaccc caccagatgt ggagcctgag
     1561 cttgaacaca tccggctccg aaagaaggac caatttgtcg aatccgttta tgtgtatgta
     1621 cgattgcgcg cccgcgcgtt gcacgagctc cgagtctgcg cagaccgccc cgagtatgca
     1681 tcggagccgc tgcttttccg cgagccatcg cacgaccggg agcagctgtt cgctcaaggc
     1741 tccgcgcgcc tcatacacga agtcgcgcaa ccaggttcgc agtgccggcg ccgattcgac
     1801 cggtgcctcc gtgatgttcg tcatgctgat ccctcggtca tagatgtccg tatgacggtg
     1861 acgccggcga gccgccgaaa cgatcgcgtc gcgttcatgg attggcaggc agggcctcgt
     1921 gacagggccc ggcggggaac tcggggtcgt tcttcctcgc taacgtggtc tcgagatccg
     1981 gaaaggcagc atgaaccgcc gaggccaatc gcgtcgattc ggcgcgtgcg gtctcgcccc
     2041 tccattcgac ctcgacgcag cgaaacagac ggcaggatga ctctcccagc gcttgctcca
     2101 gcgcagccga ggagaccggc tcacgcgcga gcagggctct gccaatcgct gcggcgaagg
     2161 cggcaagagg cggctggcgg acggggcgca gccagcgacc ttcctcgaag ctcccctgga
     2221 cgaacggggt cgtcgcgacg aggaaagcca gcggctggtc ttcctgcgac gagaccgcgg
     2281 gcgcatggct cgagagagtc ttgggcaggc aaactggctc gatgctgcgg gtgcgtttga
     2341 atatcaccga ggacacacgc tccgaagacc ggctcatggc ttcgagaaaa gccgcgacga
     2401 ccggagggtg cgcgaagctg tagaccagca tgacctcctc gccgcgagcg gccagcgtcc
     2461 caccatcggt ctccagaagg gctctctcgc ccgtgtctcg cttggagatc ggaagcagga
     2521 acgtggcgcc gtgcgcatgc gtgagacgca gggcgtgcga gtcgggcgaa gtgaggcccc
     2581 cgggttccgc cagatagata tcgacggcgg ccacgggcgc ggcgaggcgg agaccgtgtg
     2641 ttcgcaggtt ggcctcggga tctgacggac gctccgacgg tgccgcggcc gcggtccatt
     2701 cccagcactg gttgagagcg acgataccga ccgcgtcccg atccgatgcg cgcaggaact
     2761 tgccgagcag cgcctgggcg cgccgggcgg cttccggcga tacggtctcc ggggcatggc
     2821 gcaggatcgc cgcgacgtcg atgggcgtgc cgtgggcgag tcgctctagc cgctccttga
     2881 gaccggccgc ggtcgggggc ggcgacggcg acgtcatggc cgcgtctcga gcgggcggtc
     2941 catggggacg aggttgtgcc cgggccggta aggtgattcg agcagtcccc ggcggacgaa
     3001 cccctgtgcc tcgagaaatg cgagctggcg ttcgtcgcgc tcgtgggcga acgtatagag
     3061 cttccggccg ccgagcgcgg cgacctcggc gatcgctgtc tggagcaact cctcctgggc
     3121 gagcgccgtg ccgatgaggg gatagagctt ggcgggcccc ccacgcttgg gcgtgagaat
     3181 gagaaggccg gatcggtggc cgtcatgtcc gcgggcaacc aggatgcgcg tcgacttacg
     3241 ctgatagagg tggtcgcccc cggccaaccc gcgccagtgc gccgcgactt gagctcgggg
     3301 aagctcgtcg tccgcatcat cgtgccattg gcggcagact ttgtaggctg actggaagta
     3361 gccccagtcc tcgtcttcat aaaggccgat ccggaacgcc tggccctgcc cggattcgag
     3421 cgaggacgac acggaggcca ccgcgagcgg cggcttgtcg aggtgcatgc cccagaccgt
     3481 ctccgtcacg ccggtacgga aatggtcggg aaaagcggct tcctccttga gaccagcttc
     3541 aggggcgacg ccgaggagga ccgccgccat tccggcgttc cggcgcggga acgtaccgta
     3601 gagcttgcgg cggccggtca atcgcgcgat cgtcagggcc gcttcaaaaa gccgcgtcgc
     3661 caccacgcgc ttgtgcggcg cgccgcggtc gatgaggagg gggccgagct tcgtggaccc
     3721 accgcgcttg tggttcaggc agagcgcgcc cgtggggcgg cccacgcctt cgcaaacgag
     3781 gatgagcttg tgagtcgtgt gcagccccaa cggatcgccc tgctcgtgac cgatcagctg
     3841 atccaacaac gcgggtgcgc cgtcctcatg tccgtcgtag aaggccggca agatgcggga
     3901 ccagtcgaga aggagcgcgt aatcgtcccg ccggtagagc cggatgccgg ggaggcccgc
     3961 cgcttctact agatcgtcgg gggccatcag atcgtcgggg gctatcgttc tcactgtgga
     4021 gagcactata cgtccggtgt ctttcaattt catgtgatca acgacacata cgaggttgtg
     4081 ccacgagtgt gctcgtgttc cccaatatgg tccaatcagc gcacggggcc ttgatgtccg
     4141 atattcgaat atggtcgtag tacggcggac ggacctcgcg cttgctcgcg cagggccacg
     4201 gcgaagacgc tcgggatgac gatgccgagg cgcgcacctt cctcgccagc tcttcgaccc
     4261 tcttccggtg gcgctgctgc cgcgcgtcga ttccccacat cgggcgtgcc tcgtgatcat
     4321 gatgtcgatc gacgaggtgc cga
//
LOCUS       DASVOR010000330         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207643,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000330 DASVOR010000000
VERSION     DASVOR010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207643"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..937
                     /locus_tag="VF516_06820"
     CDS             209..937
                     /locus_tag="VF516_06820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8107425.1"
                     /translation="MRDLHFAYRGDPREVLAGVNLQVAAGRRVLVIGANGAGKTTLLR
                     ILAGKHMVDPERVRVLGASAFHEPSLSGRIEFLGGRFPFDVDLRVDEILARQVGVDPA
                     RRDRLIEVLGVDVGWHMHAVSDGQRRRVQLLLGLLRPREVLLLDEITTDLDLIARQDL
                     LAFLREETERPGQPTTILYATHIFDTLDRWATDVVYVAAGKVVIDAPIGAVPELAADP
                     LVTVVERWLRRDLGRSAVAGRREP"
     gene            970..1554
                     /locus_tag="VF516_06825"
     CDS             970..1554
                     /locus_tag="VF516_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107426.1"
                     /translation="MGTRTSELKVPTMVVTVRLAIVGASPSAVEMFVPDVPRRGRGQL
                     LDDLATQLDGAARFVPVRTTGRVRLVAKHAIAWISIERHETGGGDPGFAGGAGGGAPR
                     GIDRRDPEETPVEPAEIPEELTLFDHQHFVEVELAHGTKLIGTLLDSAPADHARVADH
                     LNHLGRWLRLWTTDQHYLINVQQVVAVTELGEMW"
     gene            1554..>2256
                     /locus_tag="VF516_06830"
     CDS             1554..>2256
                     /locus_tag="VF516_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107427.1"
                     /translation="MSRLDVYLRSIKQLGALGAVLTSGQAVTLRFPTGDRHATQITAH
                     DQLVSLVREIAPPAALEQIDRGRPASFELEASGGRWAIQVVPRPGAWHVAIDPIDPSP
                     SSSSSSSSSSSPSSGGAAGPPVRPTPRPGTMGPGAGEASEMANERGPAGVHTTSGGSA
                     LLDHLTAGGRAVYASDIYLNAGVPAFQRIGGELAATADRAVIDGDTLARELGQVAPSE
                     ARSAWAERGLAVFAYG"
BASE COUNT          280 a          766 c          905 g          305 t
ORIGIN      
        1 gggtcggagc aggcgcggcg gtggtcgggg gcgcggcggc gcgggggtcg agcgccgtgc
       61 gcagcgcgcg gttggcgagg tcgagccaca gcgcgagcga cgcgctggcc agcgacggcg
      121 cgccgagcag ccaggtggcg cgtgccatgg cgcaaagcta gcatgcgcgg tgatgacggc
      181 tgtcgcggca tctgctcctg tgatcgaggt gcgtgatctg catttcgcgt atcgcgggga
      241 tccgcgcgag gtgctggccg gcgtgaacct gcaggtggcg gccggacggc gcgtgctggt
      301 gatcggggcc aacggcgccg gcaagaccac gctcttgcgc atcctcgccg gcaagcacat
      361 ggtggatccc gagcgcgtgc gggtgctcgg ggcgtcggcg ttccacgagc cgtcgctgtc
      421 gggccggatc gagtttctcg gcgggcggtt cccgttcgac gtcgacctgc gggtcgacga
      481 gatcctggcg cgccaggtcg gcgtcgatcc ggcgcggcgc gaccggctga tcgaggtgct
      541 cggggtcgat gtcggctggc acatgcacgc ggtgtccgac gggcagcgcc ggcgggtcca
      601 gctgctgctc gggctgctcc ggccgcgcga ggtgctgctg ctcgacgaga tcacgaccga
      661 tctcgatctg atcgcgcgcc aggacctgct cgcgttcctg cgcgaggaga ccgagcggcc
      721 gggccagccg acgacgatcc tgtacgccac ccacatcttc gacacgctcg atcgctgggc
      781 gaccgacgtg gtctacgtcg ccgccggcaa ggtggtgatc gacgcgccga tcggggcggt
      841 gcccgagctc gcggcggacc cgctcgtgac cgtggtggag cgctggctcc ggcgcgacct
      901 ggggcgcagc gcggtcgccg ggcgccgcga gccgtagccg ccgcgtgccg gggcgagtac
      961 gatgcgcgcg tggggacgcg cacgagcgag ctcaaggtcc cgacgatggt ggtcaccgtg
     1021 cgactcgcga tcgtgggcgc gtcgccgtcg gcggtcgaga tgttcgtgcc cgacgtgccg
     1081 cggcgcgggc gcggccagct gctcgacgac ctggcgacgc agctcgacgg cgcggcgcgg
     1141 ttcgtgccgg tgcgcacgac gggccgggtc cggctggtcg ccaagcacgc gatcgcgtgg
     1201 atctcgatcg agcgccacga gacggggggt ggggatcccg gcttcgccgg cggggcaggg
     1261 ggcggagccc cccgtgggat cgaccgccgg gatcccgagg agacgcccgt cgagccggcc
     1321 gagatcccgg aggagctgac gctgttcgac caccagcact tcgtcgaggt cgagctcgcg
     1381 cacggcacca agctgatcgg cacgctgctc gacagcgcgc cggccgacca cgcgcgggtc
     1441 gccgatcacc tcaaccacct ggggcgctgg ctgcggctgt ggacgaccga tcagcactac
     1501 ctgatcaacg tgcagcaggt ggtcgcggtg accgagctcg gggagatgtg gtgatgagcc
     1561 ggctcgacgt ctacctgcgc agcatcaagc agctcggcgc gctgggggcg gtgctgacgt
     1621 cgggccaggc ggtcacgctg cggttcccga ccggcgatcg ccacgccacg cagatcacgg
     1681 cgcacgacca gctggtgtcg ctggtccgcg agatcgcgcc cccggcggcg ctcgagcaga
     1741 tcgaccgcgg gcggccggcg agcttcgaac tcgaggccag cggcgggcgg tgggcgatcc
     1801 aggtcgtgcc gcggcccggc gcctggcacg ttgccatcga cccgatcgat ccgtcgccgt
     1861 cgtcctcgtc ctcgtcctcg tcctcgtcct cgccgtcgtc cgggggcgcg gccggtccgc
     1921 cggtgcggcc gacgccgcgg ccggggacga tggggccggg ggccggcgag gcgagcgaga
     1981 tggcgaacga gcgcgggccg gcgggtgtgc ataccaccag cggtggcagt gcgttgctcg
     2041 accacctgac agccggtggg cgcgcggtct acgccagcga catctacctg aacgccgggg
     2101 tgcccgcgtt ccagcgcatc ggcggcgagc tggcggcgac ggcggaccgc gcggtgatcg
     2161 acggcgacac gctggcgcgc gagctcggcc aggtcgctcc cagcgaggcg cgcagcgcgt
     2221 gggccgagcg cggcctggcg gtgttcgcct atggcg
//
LOCUS       DASVOR010000331         2001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265874,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000331 DASVOR010000000
VERSION     DASVOR010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265874"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1649)
                     /gene="ligD"
                     /locus_tag="VF516_06835"
     CDS             complement(<1..1649)
                     /gene="ligD"
                     /locus_tag="VF516_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8107428.1"
                     /translation="MAHDDGLDTYRAKRTPGRTPEPMASTAAAGRGPRMFVVHQHDAT
                     RMHWDLRLEIDGVLCSWAVPKEPSMDPEDKRLAVKVENHPLDYVHFEAVIPDGNYGAG
                     PMIAWDRGLFRPLVDPAQGLVDGEIKFELYGYKLRGAFTLVHTGKGRRGRSSGRGSND
                     WLLIKKRDEPAQAFLATGQPLPDTSVLSGLTIDELAAGAPRHQAVIAELAELGAPRRA
                     IDPDRCELMLCHTADAPFSSDDWVFELKYDGYRLAAFGGAGQAALRYRSGQDPTLRYP
                     ELGSALRALPIPGLVLDGEVVVLDAEGKPDFQRLAIRAQTHRASEIQRAALAGPVTYM
                     VFDLLGAAGYDLRGLPLLVRKSLLARILPALGPLKLAEHIPSQGEALLAQVAARGLEG
                     VVAKRADSPYRPTRSRDWLKIKREPEGDFAVCGYTAPRRSRLGLGALHLCVRQDGAWR
                     WAGKVGSGLDDQLLTQLRAELDARPAWQPTFPRPEGTADVRWVEPELVVQVRYGEWPD
                     DGPLRFPVFVRLRRDKTAADCTARPRPARGAGRIGDGDGDG"
BASE COUNT          257 a          738 c          732 g          274 t
ORIGIN      
        1 tcgccgtcgc cgtcaccgtc gccgatccgg ccggcaccgc gcgcggggcg cggcctcgcc
       61 gtgcagtccg ccgcggtctt gtcgcggcgc agccgcacga acaccgggaa ccgcagcggc
      121 ccgtcgtcgg gccactcgcc gtagcggacc tggaccacca gctcgggctc gacccagcgc
      181 acgtcggcgg tgccctcggg acgcgggaac gtcggctgcc acgccggccg cgcgtcgagc
      241 tcggcgcgca gctgggtcag gagctgatcg tcgagccccg agccgacctt gccggcccac
      301 cgccacgcgc cgtcctggcg cacgcacagg tgcagcgcgc cgagccccag ccgcgagcgc
      361 ctgggcgcgg tgtagccgca caccgcgaag tcgccctcgg gctcgcgctt gatcttgagc
      421 cagtcgcgcg atcgggtcgg gcggtacggc gagtccgcgc gcttggcgac cacgccctcg
      481 aggccgcgcg ccgcgacctg ggcgagcagc gcctcgccct gcgacgggat gtgctcggcg
      541 agcttgagcg ggccgagcgc cggcaggatc cgggcgagca ggctcttgcg gaccaggagc
      601 ggcaggccgc gcaggtcgta ccccgcggcg ccgagcaggt cgaacaccat gtacgtcacc
      661 ggtccggcga gcgcggcgcg ctggatctcg gaggcgcggt gggtctgggc gcggatcgcg
      721 agccgctgga agtccggctt gccctcggcg tcgagcacca cgacctcgcc gtcgagcacc
      781 agcccgggaa tcggcagcgc gcgcagcgcc gagccgagct cggggtagcg cagcgtcgga
      841 tcctgccccg agcggtagcg cagcgcggcc tggccggcgc cgccgaacgc cgcgaggcga
      901 tagccgtcgt acttgagctc gaacacccag tcgtccgacg agaacggcgc gtccgcggtg
      961 tggcacagca tcagctcgca gcggtcgggg tcgatcgcgc ggcgcggcgc cccgagctcg
     1021 gcgagctcgg cgatcaccgc ctggtggcgc ggcgcaccgg cggcgagctc gtcgatggtc
     1081 agccccgaca gcacgctggt gtccggcagc ggctggccgg tggcgaggaa cgcctgggcg
     1141 ggctcgtcgc gcttcttgat cagcagccag tcgttcgagc cgcggcccga gctgcggccg
     1201 cgccggccct tgccggtgtg gaccagcgtg aacgcgccgc gcagcttgta gccgtagagc
     1261 tcgaacttga tctcgccgtc gaccaggccc tgcgcggggt cgacgagcgg ccggaacagc
     1321 ccgcggtccc acgcgatcat cgggccggcg ccgtagttgc cgtcggggat caccgcctcg
     1381 aagtggacgt agtcgagcgg gtggttctcg accttgaccg cgagccgctt gtcctcgggg
     1441 tccatcgacg gctccttggg caccgcccac gagcacagca cgccgtcgat ctcgagccgg
     1501 aggtcccagt gcatccgggt cgcgtcgtgc tggtggacga cgaacatccg cggcccgcgc
     1561 ccggccgcgg cggtgctcgc catcggctcg ggtgtgcggc cgggggtgcg cttggcgcgg
     1621 taggtgtcga gtccgtcgtc gtgtgccatg ggaaaatcgc tgccccgatc tcgccaagtc
     1681 ttccacagga tctcgcagct ggcgcgggcc gcgtgcgtcg ccgccccggc cggcttgcca
     1741 ggtcgcgctg cccaagggtt cagtgccggt tcaggtgccg gtccgggtgc cagagcgtgc
     1801 cgccccggcg cccgccggac tccgcattcg agaattcgtc gcttcgggcg tcagtcggta
     1861 caattatttt cccatgcccg cccgctcgat cagcacggcc acgatctcgt tcggtctggt
     1921 gtcgatcccg accaagctct acacgaccaa cgaggccgcc ggcgacatcc acttcaacat
     1981 gctgcacgac gccgacggcg c
//
LOCUS       DASVOR010000332         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_382895,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000332 DASVOR010000000
VERSION     DASVOR010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382895"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="VF516_06840"
     CDS             complement(<1..1115)
                     /locus_tag="VF516_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8107429.1"
                     /translation="MLLLLLGLGGRAWAGKPPIAILGLEVYDNGGGIDPETTKAAKEL
                     TAALRDRAKAGTGPFTPVQGEKELIDEKLLNNCDSEAPTCMATIGTGLGAEVLMYGKI
                     EKATQSGQGVYRVSIKLLSVTRKQLTASTVETLPVTDATGVRAQMYAKTWYAKLAGAG
                     SGGTLVIRANIDRGTVLVDDDMKGTLASGTLTLTGIAEGRHTLAVEASTRDYQRYEAQ
                     VTVRPGETTTQNVTMVAMPRKLAELQPKETITREGTVGQPQPKTNVWKPVFFGTAAIT
                     AAGAGFTVYEWVQGRNNASAITKDGAFNTTVGVGSPMKQVGNSQCNSSQVTDFASTHP
                     GDPDVKHFNDACNNYKYQKIGWVVTGVIGAAAIGSFI"
     gene            1241..>2191
                     /locus_tag="VF516_06845"
     CDS             1241..>2191
                     /locus_tag="VF516_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8107430.1"
                     /translation="MRTLSARILLGFIALTITFGVFAATIIVNLREVEDQARLILNGY
                     VPLTMASSDLERQQGYLKTYLDQGIMDATRAREVVVTIDHLRSNREFPLNEIVNKVGS
                     LDRLATSDGQAAAEGAAQFSRTGPVVDELKQAVGDVAPLYKTLLAAPPLRVPSRTDPS
                     FQAAYDALDADHRAAYDTLLQLRDKEKQVSSLVATIKSNFGTRTTLTRNWLANNERTI
                     LVRALYLGIVTVLLGLVITAWVAVTLRPLRRLREGARRIAAGDYGKRISETGPTEIAD
                     LAREFNSMGRAVQEREEEKLRAARIAIAGKMAGQVAHEVRN"
BASE COUNT          321 a          778 c          721 g          371 t
ORIGIN      
        1 atgatgaacg agccgatcgc cgcggccccg atgacaccgg tcacgaccca gccgatcttc
       61 tggtacttgt agttgttgca ggcgtcgttg aagtgcttca cgtcggggtc gcccgggtgg
      121 gtgctcgcga agtcggtcac ctgcgagctg ttgcactggc tgttgccgac ctgcttcatc
      181 ggcgagccga caccgaccgt cgtgttgaac gcgccgtctt tggtgatcgc gctggcgttg
      241 ttcctgccct gcacccactc gtagaccgtg aacccggcgc ctgccgccgt gatggccgcc
      301 gtcccgaaga acaccggctt ccagacgttg gtcttcggct gcggctggcc caccgtgccc
      361 tcgcgcgtga tcgtctcctt cggctgcagc tcggccagct tcctgggcat cgcgaccatc
      421 gtcacgttct gcgtcgtggt ctcgccgggg cggaccgtga cctgggcctc gtagcgctgg
      481 tagtcccggg tcgatgcctc gaccgccagc gtgtgcctgc cctcggcgat gccggtgagc
      541 gtgagcgtgc cgctcgccag ggtgcccttc atgtcgtcgt cgaccaggac ggtgccgcgg
      601 tcgatgttcg cgcggatcac gagcgtgccg ccggagcccg cgccggcgag cttggcgtac
      661 caggtcttgg cgtacatctg ggcccggacg ccggtcgcgt cggtcaccgg cagggtctcg
      721 accgtcgacg ccgtgagctg cttgcgggtg acgctgagca gcttgatcga gacccggtag
      781 accccctggc cgctctgcgt cgccttctcg atcttgccgt acatcagcac ctcggccccg
      841 agcccggtgc cgatcgtcgc catgcaggtc ggcgcctcgg agtcgcagtt gttgagcagc
      901 ttctcgtcga tcagctcctt ctcgccctgc accggggtga acgggccggt gccggccttc
      961 gcgcgatcgc gcagcgcggc cgtgagctcc ttggccgcct tcgtggtctc ggggtcgatc
     1021 ccgccgccgt tgtcgtagac ttccagaccg aggatcgcga tcggcggctt gccggcccac
     1081 gccctgcctc ccagacccag cagcagcagg agcacacaag cagaggaaag aactcgggtc
     1141 atcgggggtt cctcgcgggg tagtatacac atccccgccc ccgaaaacgc gggtctggct
     1201 cgcccgactc ccccgagcta ccgttgaggt gacgcaggtc atgaggacgc tatcagcccg
     1261 gatcctcctc ggttttatcg ccctgacgat cacgtttggc gtgtttgccg cgacgatcat
     1321 cgtcaacctc cgcgaggtgg aagatcaggc gcgactgatc ctcaacggct acgtcccgct
     1381 gaccatggcg tcgagcgatc tggagcggca gcaggggtac ctcaagacct acctcgacca
     1441 gggcatcatg gatgcgacac gggcgcgcga ggtcgtcgtc acgatcgacc acctgcgctc
     1501 caaccgcgag ttcccgctca acgagatcgt caacaaggtc ggcagcctcg atcggctcgc
     1561 cacctcggat ggccaggccg ccgcggaggg cgcagcgcag ttctccagga ccggcccggt
     1621 cgtcgacgag ctcaagcagg cggtcggcga cgtcgcgccg ctgtacaaga ccctgctggc
     1681 cgcgcccccg ctgcgcgtcc cgagcaggac cgatcccagc ttccaggccg cctacgacgc
     1741 gctcgacgcc gatcaccgcg ctgcgtacga cacgctgctg cagctgcgcg acaaggagaa
     1801 gcaggtctcg tccctggtcg ccacgatcaa gagcaacttc ggcacccgca ccacgctcac
     1861 caggaactgg ctggccaaca acgagcgcac gatcctggtg cgcgcgctct acctcggcat
     1921 cgtcaccgtg ctgctcggcc tggtgatcac cgcctgggtc gcggtcacgc tgcggccgct
     1981 gcgccggctg cgcgagggcg cgcggcggat cgccgccggc gactacggca agcggatctc
     2041 cgagaccggc ccgaccgaga tcgccgatct cgcccgcgag ttcaactcga tgggccgcgc
     2101 ggtgcaggag cgcgaggagg agaagctgcg cgcggcgcgg atcgcgatcg ccggcaagat
     2161 ggccggccag gtcgcccacg aggtccgcaa c
//
LOCUS       DASVOR010000333         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207648,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000333 DASVOR010000000
VERSION     DASVOR010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207648"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VF516_06850"
     CDS             complement(<1..301)
                     /locus_tag="VF516_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107431.1"
                     /translation="MVALEVLDRLVVRGDRLLEVGALELGAAVHRGDAELERHERVAH
                     VVGPVLGEVRDALVERRVIEVLRDLELLAHDLHELDPAARVVAGEERHHHALAVGV"
     gene            684..1229
                     /locus_tag="VF516_06855"
     CDS             684..1229
                     /locus_tag="VF516_06855"
                     /inference="COORDINATES: protein motif:HMM:NF018813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HEX8107432.1"
                     /translation="MALPATLRRFEIALADADRELYEQLDWRVAQHPSESERYLVARV
                     IARALEHGDGVEFSRGGVSDDDEPAIVQRDLRGDLRAWIEVGSPSPDRLHKATKLAPR
                     VAVYAWKNAEQLAEAIAERGVHRAGEIALRALPIELLDGVVATLDRVNRWDLSVTGGV
                     LYLAVGAAQFEGAVRPVAIAG"
     gene            1284..2000
                     /locus_tag="VF516_06860"
     CDS             1284..2000
                     /locus_tag="VF516_06860"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8107433.1"
                     /translation="MRIYFVGSHATGKTTLCRHVSRRYGLPMITEVARAVLAEMETGL
                     DALRTDMDLVAEYQERVFARQVAVERQHADRFVSDRAFDNLAYVAEHTTNAAAMMNDQ
                     RFRDYMRWVSEGVVFFLRPHQSLLKDDGVRAGVSWDSVLRIDGMVKLMLEQHGIAYLP
                     IESVSMQERVRAVEFVLARCGLQLPARPAARSLARSTAGPAARMGADLPATAPPPAEV
                     LRSGVESWPHALVDHGDHEA"
BASE COUNT          330 a          785 c          797 g          304 t
ORIGIN      
        1 ggacgccgac cgccagggca tggtgatggc gttcctcgcc ggctaccacc cgcgcggcgg
       61 gatcgagttc atggagatca tgggcaagga gctcgagatc ccgcagcacc tcgatcaccc
      121 gacgttcgac gagcgcgtcg cgtacctcac cgagtactgg accaacgacg tgcgctacgc
      181 gttcgtgtcg ttcaagctcg gcgtcgccgc gatggaccgc ggcgccaagc tcgagggcac
      241 cgacctcaag caggcggtcg ccgcgtacga cgaggcggtc gaggacttca agcgctacca
      301 cgcgatgctg ccatcgctca aggaggcatc caacgacctc ggcgtcgcct acaccaagct
      361 cggcgtgctc gcgatggccg ccgactcgcc gctcggccgc tggcagaccc ggttctcgct
      421 cgagcgcagc tcggcggtga gctacgtcgg cctggcgcgc gacgaggagt cgaggacccg
      481 cggcggcgac aaggcccgcc tgccgcagca gctgcgcgag gcgatcgccg cgttcaagga
      541 ggcgctcgcg ctcgacgaga actacagcaa ggcgcggctc aacctcgccg ccgcctacaa
      601 gctctacccc ggcggcccgt gggccaaggc cgccgaggcg ccgccggcaa gctgtagcgg
      661 cggaagcggc gtaccatggt ggcatggcgc tgcccgccac gcttcgcagg ttcgagatcg
      721 ccctcgccga cgccgatcgc gagctctacg agcagctcga ctggcgggtc gcgcagcacc
      781 cgtcggagag cgagcgctac ctggtcgccc gggtgatcgc gcgcgcgctc gagcacggcg
      841 acggcgtcga gttcagccgc ggcggggtgt ccgacgacga cgagccggcg atcgtgcagc
      901 gcgacctgcg cggcgacctc cgggcgtgga tcgaggtcgg ctcgccgtcg ccggatcgcc
      961 tgcacaaggc gaccaagctc gccccgcgcg tcgcggtgta cgcctggaag aacgccgagc
     1021 agctcgccga ggcgatcgcg gagcgcggcg tgcaccgcgc cggcgagatc gcgctgcgcg
     1081 cgctgccgat cgagctgctc gatggcgtcg tggcgaccct cgaccgggtc aaccgctggg
     1141 acctgtcggt caccggcggc gtgctgtatc tcgcggtcgg cgcggcgcaa ttcgaaggcg
     1201 cggtgcgccc ggtcgcgatc gcgggctgag cgcggcgggg ccaccgtcga accgctgtca
     1261 gatcgatcgg gtatggctgg cgggtgcgga tctacttcgt cggcagccac gcgaccggga
     1321 agaccacgct gtgccggcac gtcagccggc gctacggcct gccgatgatc accgaggtcg
     1381 cgcgcgccgt gctcgccgag atggagaccg ggctcgacgc gctgcgcacc gacatggacc
     1441 tcgtcgccga gtaccaggag cgcgtgttcg cccgccaggt cgcggtcgag cgccagcacg
     1501 cggaccgctt cgtgtcggac cgcgcgttcg acaacctggc gtacgtcgcc gagcacacca
     1561 cgaacgcggc cgcgatgatg aacgaccagc ggttccgcga ctacatgcgg tgggtcagcg
     1621 agggcgtcgt gttcttcctc cgcccgcacc agtcgctgct caaggacgac ggcgtccgcg
     1681 ccggcgtgtc gtgggactcg gtgctgcgga tcgacggcat ggtcaagctg atgctcgagc
     1741 agcacggcat cgcgtacctg ccgatcgaga gcgtgtcgat gcaggagcgg gtgcgcgcgg
     1801 tcgagttcgt gctcgcgcgc tgcgggctgc agctgccggc ccggcccgct gcgcgatccc
     1861 tggcccgatc cacggccggg cccgccgcgc ggatgggcgc cgacctgccc gcgaccgcgc
     1921 cgcctcccgc cgaggtgctc cggtccggtg tcgagtcgtg gccgcacgcg ctggtcgacc
     1981 acggcgacca cgaggcgtga tcggaccccg gcgcccggct tgacatcgaa tatttcctgg
     2041 atatattgcg gatatgtcga agaagacacc gccggaggcg gcgctctatg tccggctacc
     2101 cgcgaccgcg gtcgacaagc tcgatcgcac cgccgaggcg ctcggcgtgc acaagaagga
     2161 tctggtcgcc ggcctggtct cgaagtacgt cgatcccgac acccggcagg ggctga
//
LOCUS       DASVOR010000334         3475 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_119883,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000334 DASVOR010000000
VERSION     DASVOR010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3475
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119883"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF516_06865"
     CDS             complement(<1..253)
                     /locus_tag="VF516_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107434.1"
                     /translation="MQDRVIVLVEDNANDEELTLRAFRKSNIGNRVVVLRDGAEALDY
                     FFCRGAHAGRPASEIVQVVLLDLKLPKVDGLDVLRALRAD"
     gene            complement(238..1944)
                     /locus_tag="VF516_06870"
     CDS             complement(238..1944)
                     /locus_tag="VF516_06870"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8107435.1"
                     /translation="MADDGTSTPRLERLRDGLRLVSTITQAFAESTSDYHRLLDAIAR
                     HIAEAIPDTCIVNLVSGDTITPVAIHERVPDGAPRLRYARDQPYPMRIAGVSAEVLAH
                     GPLFMPRIDLETLAAQMTPDGVDQLRRVDTTGLLVVPLATRGERLGVLWILRRGHRHP
                     PLDELDREIVQDLANHAALAILNARLIQRLEHSERLRAAEDRAVQASSLLDAIVENIP
                     DMVFVKDAATLSFVRLNRAGEQLLGLSRDQLIGKTDFDFFPASEAEFFVAKDRETLAA
                     NRVVEIAEEPIQTPRGTRWLHTKKVPLLDAAGRPQYLLGISHDITERKQAIAELSAAK
                     STAEDANRELESFSYSVAHDLRTPLRAIDGFSQALAEDYGDRLDAEGRRYLARVRESA
                     QRMAELIDDLLTLSRVTRSELRRDRVDLSALAHTVLATLQRLDPERRVEVVIAPGLVV
                     DADPHLAAIMLDNLLGNAWKFTAKRGDARIEVGQTICDGIVAYYVRDNGAGFDMAYRD
                     KLFGVFQRLHPEAEFPGTGIGLATVARIAQRHRGRIWAEGTPGAGATFYFTLTEQPCK
                     TA"
     gene            2267..>3475
                     /locus_tag="VF516_06875"
     CDS             2267..>3475
                     /locus_tag="VF516_06875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107436.1"
                     /translation="MTALGDPGSLPPKLGRYELITRIGQGGMAEVQLALQRGPAGFEK
                     LVVVKLVHENLSSQKPFVDMLLDEARVAALVKHPNVVDIYDLGEADGRYFIAMEYLEG
                     EPLLAVLRAGRDGQRLDVLSTARLIADTAEGLDAAHELRSMAGDPIELVHHDVSLGNI
                     VVLYNGQVKLVDFGVAKATQSATARTKVQGKFGYMAPEKLRGGPGDRRSDIWSLGCVL
                     WEALTLRRLFKGGNDPDTMQQVLDIPILAPSKVNGDVPADFDPIALRALERDPGKRYA
                     TAKAMAAELEEVLRRRGYAAKNDVIARYMQVTFAAHIAARKKLLQEVSSKGRPSPDVL
                     EAAFNLGALASGSPVLPTSESWPRTPSIAKPARPDEPAEPVQTAAPAAGSAAGDEATR
                     VERPMVDVRRA"
BASE COUNT          494 a         1193 c         1282 g          506 t
ORIGIN      
        1 cgtcggcgcg cagcgcgcgc agcacgtcga gcccgtcgac cttggggagc ttgaggtcga
       61 gcagcacgac ctggacgatc tcgctcgccg ggcggccggc gtgggcgccg cggcagaaga
      121 agtagtcgag cgcctcggcg ccatcgcgca gcacgacgac gcggttcccg atgttgctct
      181 tgcggaacgc gcgaagcgtg agctcctcgt cgttcgcgtt gtcctcgacg aggacgatca
      241 cgcggtcttg catggctgct ccgtcagcgt gaagtagaac gtcgcgcccg cccccggcgt
      301 accctcggcc cagatccggc cgcggtggcg ctgcgcgatc cgcgcgaccg tggcgagccc
      361 gatcccggtg ccggggaact cggcctcggg gtgcaggcgc tggaacacgc cgaacagctt
      421 gtcgcggtac gccatgtcga acccggcgcc gttatcgcgc acgtagtacg cgacgatgcc
      481 gtcgcagatc gtctggccga cctcgatccg ggcgtcgccg cgcttcgcgg tgaacttcca
      541 cgcgttgccc agcaggttgt cgagcatgat cgccgcgagg tgggggtcgg cgtcgacgac
      601 caggccgggc gcgatcacga cctcgacccg gcgctcgggg tcgagccgct gcagcgtggc
      661 gagcacggtg tgggccagcg cgctgaggtc gacccggtcg cggcgcagct cgctgcgggt
      721 gacccgcgac agcgtcagca ggtcgtcgat cagctcggcc atccgctgcg cggactcgcg
      781 gacccgcgcc aggtagcgcc ggccctcggc gtcgagccgg tcgccgtagt cctcggccag
      841 cgcctggctg aacccgtcga tcgcgcgcag cggggtgcgc aggtcgtgcg cgaccgagta
      901 gctgaacgac tcgagctcgc ggttggcgtc ctcggcggtc gacttggcgg ccgacagctc
      961 ggcgatcgcc tgcttgcgct cggtgatgtc gtgcgagatc ccgagcaggt actgcggccg
     1021 gccggcggcg tcgagcagcg ggaccttctt ggtgtgcagc cagcgcgtgc cgcgcggggt
     1081 ctggatcggc tcctcggcga tctcgaccac gcggttggcc gccaaggtct cgcggtcctt
     1141 ggcgacgaag aactcggcct cgctggccgg gaagaagtcg aagtcggtct tgccgatcag
     1201 ctgatcgcgg gacagcccga gcagctgctc gccggcgcgg ttgagccgca cgaacgacag
     1261 cgtcgcggcg tccttgacga acaccatgtc ggggatgttc tcgacgatcg cgtcgagcag
     1321 gctggacgcc tggaccgcgc gatcctcggc ggcgcgcagc cgctcgctgt gctcgagccg
     1381 ctggatcagc cgcgcgttca ggatcgcgag cgcggcgtgg ttggcgaggt cctggacgat
     1441 ctcccgatcg agctcgtcga gcggcgggtg gcggtggccg cggcgcagga tccacagcac
     1501 gccgagccgc tcgccgcggg tcgcgagcgg gacgaccagc aggccggtgg tgtcgacccg
     1561 gcgcagctga tcgaccccgt cgggggtcat ctgcgccgcg agcgtctcga ggtcgatccg
     1621 gggcatgaac agcgggccgt gggcgagcac ctcggccgac accccggcga tgcgcatcgg
     1681 gtacggctga tcgcgggcgt agcgcagccg cggagcgccg tcgggcaccc tctcgtggat
     1741 cgcgaccggg gtgatcgtgt cgcccgacac caggttgacg atgcaggtgt cggggatcgc
     1801 ctcggcgatg tggcgggcga tcgcgtcgag cagccggtga tagtccgagg tgctctcggc
     1861 gaacgcctgc gtgatggtcg acaccagccg cagcccgtcg cggagccgct cgagccgcgg
     1921 cgtggacgtc ccatcgtcgg ccatccggtt cgactataca caacctggac cgcgttcacc
     1981 ggcgctgcgg gtccgccgtc gacgccggtc accgggaacc gcgcgttcct ccgacgagct
     2041 cagcgatcca ctggcgcgtc gtgcggcagg ccccggcggc gggaggtctt cgggcctgcg
     2101 cgccggcccg tacagtgcgg tccgcgaccc gggggctggc ttacgagcca accggacgat
     2161 gaccgcggcc tcgatcacct ttcgtggcga acgggtgagc cggatcctgc ccaacgttta
     2221 gcgacggaga gacttgggaa actctcgttg cgttacaata tttttcgtga ctgcactggg
     2281 ggatccaggc tctctgccgc ccaagctcgg gcggtatgag ctgatcaccc gcatcggtca
     2341 gggcgggatg gccgaggttc agctcgccct gcagcgcggc ccggccgggt tcgagaagct
     2401 cgtggtggtc aagctggtgc acgagaatct ctcatcgcag aagccgttcg tcgacatgct
     2461 gctcgacgag gcgcgcgtcg ccgcgctggt caagcacccc aacgtggtcg acatctacga
     2521 cctcggggag gccgacggcc gctacttcat cgcgatggag tatctcgagg gcgagccgct
     2581 gctcgcggtg ctccgggcgg gccgcgacgg ccagcgcctc gacgtgctat cgaccgcccg
     2641 gctgatcgcc gacaccgccg aggggctcga cgccgcgcac gagctgcgct cgatggccgg
     2701 cgacccgatc gagctggtgc atcacgacgt gtcgctcggc aacatcgtcg tcttgtacaa
     2761 cggccaggtc aagctggtcg acttcggggt cgccaaggcg acgcagtcgg cgaccgcgcg
     2821 caccaaggtc cagggcaagt tcggctacat ggcgccggag aagctgcgcg gcggtcccgg
     2881 cgatcgccgc agcgacatct ggtcgctcgg ctgtgtgctg tgggaggcgc tgaccctgcg
     2941 ccggctgttc aagggcggca acgaccccga cacgatgcag caggtgctcg acatcccgat
     3001 cctcgcgccg tcgaaggtca acggcgacgt ccccgccgac ttcgacccga tcgcgctgcg
     3061 cgcgctggag cgcgatccgg gcaagcgcta cgcgacggcg aaggcgatgg cggccgagct
     3121 cgaggaggtg ctgcgcaggc gcggctacgc ggcgaagaac gacgtgatcg cccggtacat
     3181 gcaggtgacg ttcgccgcac acatcgcggc gcgcaagaag ctgctgcagg aggtgtcgag
     3241 caagggccgg ccgagcccgg acgtcctcga ggccgcgttc aacctcggcg cgctggcgtc
     3301 gggctcgccg gtgctgccca ccagcgagtc ctggccgcgc accccatcga tcgcaaagcc
     3361 ggcgcgcccc gacgagccgg ccgagccggt ccagaccgcc gcccccgccg cggggagcgc
     3421 ggcgggagac gaggcgaccc gggtcgagcg cccgatggtc gacgtccggc gggcg
//
LOCUS       DASVOR010000335         2075 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_558431,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000335 DASVOR010000000
VERSION     DASVOR010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558431"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VF516_06880"
     CDS             complement(<1..1119)
                     /locus_tag="VF516_06880"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEX8107437.1"
                     /translation="MKVLWSWLLELCDLDRQPTAEEGARALTRGGLEIEGMTNLGAGF
                     AGVVVAEVVAKRPHPHAEKLTLVDVITERGGAATQVVCGASNVPAPGRKVLWAQVGAP
                     LPGPDGATNTLGVKPVKGVVSPGMLCAEDELGLGDDHDGIIVLDEDDRTPLGAPAQRA
                     LGVDDWLLEVNAPANRGDVLGHLGVARELVAMLRGKLVLPDADLSELRGGDRPALDVA
                     IADAAACPRYTARVISGLRIAPSPRRIAQRLRNVGVRPISNLVDATNYVMFELGQPLH
                     AFDADTLTQGVIGVSPAVDGEKFVTLDGAERTLVPDDIVIRDGVRAVALAGVMGGLDT
                     EVTERTTRVLLEAASFAARSVRRTARRLGLHSEASHRFE"
     gene            complement(1164..>2075)
                     /gene="pheS"
                     /locus_tag="VF516_06885"
     CDS             complement(1164..>2075)
                     /gene="pheS"
                     /locus_tag="VF516_06885"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX8107438.1"
                     /translation="KKGKLSEVMKELGRLPAGDRPAVGAAANKVKQAIEDRVAARLGE
                     LADVAGKLDLARTVDVTLPARPVGGGHLHILTQVRLEAIQIFAELGFVVADGPQIETD
                     WHTFEALAIPKDHPARDMQDTFYVSEDIVLRTHTSPVQVRTMLTQPPPIRIVAPGHVY
                     RRDDDLTHSPMFTQLEGLAVDEGISFADLKGSLLHFVTRFFARDLGIRLRPSYFPFVE
                     PGAEVDMQCTFCMGSGCRLCKGTGWVEIGGAGMVDPEVFGHVQIDAEKYTGFAFGMGF
                     ERMAMLRHGVNDIKFYYEGDVRFLEQF"
BASE COUNT          292 a          752 c          736 g          295 t
ORIGIN      
        1 ctcgaagcgg tgcgacgcct cggagtgcag gccgagccgg cgcgcggtcc gccgcaccga
       61 gcgcgccgcg aagctcgccg cctcgagcag gacccgcgtg gtgcgctcgg tgacctcggt
      121 gtccagaccg cccatcacgc cggcgagcgc gaccgcacgc acgccgtcgc ggatcacgat
      181 gtcgtcgggc accagcgtgc gctcggcgcc gtcgagcgtg acgaacttct cgccgtcgac
      241 cgcgggcgac acgccgatca cgccctgggt cagtgtgtcc gcgtcgaacg cgtggagcgg
      301 ctggccgagc tcgaacatca cgtagttggt cgcgtcgacc aggttggaga tcggccgcac
      361 gccgacgttg cgcaggcgct gggcgatccg gcggggcgat ggcgcgatgc gcagtcccga
      421 gatcacgcgc gcggtgtagc gcgggcacgc cgcggcatcg gcgatcgcga cgtccagcgc
      481 cgggcgatcg ccaccgcgca gctcgctcag atcggcgtcg ggcaggacca gcttgccgcg
      541 cagcatcgcg accagctcgc gcgccacgcc gaggtggccg agcacgtcgc cgcggttggc
      601 gggcgcgttg acctcgagca gccagtcgtc gacgccgagc gcgcgctgcg ccggcgcccc
      661 gagcggcgtg cgatcgtcct cgtcgagcac gatgatcccg tcgtggtcgt cgccgagccc
      721 gagctcgtcc tcggcgcaca gcatgccggg cgacacgacg cccttgaccg gcttgacgcc
      781 gagcgtgttc gtggcgccgt ccggccccgg cagcggcgcg ccgacctgcg cccacagcac
      841 cttgcggccc ggcgccggga cgttgctcgc gccgcatacc acctgggtcg ccgccccgcc
      901 gcgctcggtg atgacgtcga ccagggtcag cttctcggca tgggggtgcg gccgctttgc
      961 gacgacctcg gcgaccacga cgccggcgaa cccggcgccg aggttcgtca tgccctcgat
     1021 ctccaggccg ccgcgggtca gcgcccgcgc gccctcctcg gcggtcggct ggcgatcgag
     1081 gtcgcacagc tccaggagcc agctccacag aaccttcatg gcgactcctt ccggcagttc
     1141 gatatccgca tcgatatccg catctagaac tgctccagga accgcacgtc gccctcgtag
     1201 tagaacttga tgtcgttgac accgtgccgc agcatcgcca tgcgctcgaa gcccatgccg
     1261 aacgcgaacc cggtgtactt ctcggcgtcg atctggacgt ggccgaacac ctcggggtcg
     1321 accatgcccg cgccgccgat ctcgacccag ccggtgccct tgcacagccg gcagccgctg
     1381 cccatgcaga acgtgcactg catgtcgacc tcggcgcccg gctcgacgaa cgggaagtag
     1441 ctcggccgga gccggatccc gaggtcgcgg gcgaagaacc gggtcacgaa gtggagcagc
     1501 gagcccttga ggtcggcgaa cgagatgccc tcgtcgaccg cgagcccctc gagctgggtg
     1561 aacatcggcg agtgggtcag gtcgtcgtcg cggcggtaca cgtggcccgg cgcgacgatc
     1621 cggatcggcg gcggctgggt cagcatggtg cggacctgga ccggcgaggt gtgggtccgc
     1681 agcacgatgt cctcggacac gtagaacgtg tcctgcatgt cgcgcgcggg gtggtccttg
     1741 gggatggcga gcgcctcgaa ggtgtgccag tcggtctcga tctgcgggcc gtcggcaacg
     1801 acgaacccga gctcggcgaa gatctggatc gcctccaggc ggacctgggt caagatgtgg
     1861 aggtggccgc cgccgaccgg gcgcgccggc agggtgacgt cgaccgtgcg cgcgagatcg
     1921 agcttgccgg ccacatcggc gagctcgccg agccgcgccg cgacccggtc ctcgatcgcc
     1981 tgcttgacct tgttggccgc ggcgccgacc gccggccgat cgcccgccgg cagccggccg
     2041 agctccttca tgacctcgga cagcttgccc ttctt
//
LOCUS       DASVOR010000336        10260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353739,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000336 DASVOR010000000
VERSION     DASVOR010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10260
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353739"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF516_06890"
     CDS             complement(<1..249)
                     /locus_tag="VF516_06890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107439.1"
                     /translation="MSSDRSAGPEPGSAIDHPGGAAPRLAAFERRALESPPPRAEKWL
                     FGPLFGGFLAALVLLGVSHAAAFFVWAGASAATVVIGIR"
     gene            complement(850..2052)
                     /locus_tag="VF516_06895"
     CDS             complement(850..2052)
                     /locus_tag="VF516_06895"
                     /inference="COORDINATES: protein motif:HMM:NF023218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEX8107440.1"
                     /translation="MTLLAESRNAFAITDGNYTIHTAVSGKCLEVPGSNTADGAPLQE
                     WSCNDTQAQIFEVTQLGGGFYKLVNVNSKKSLDIRNASTQENAPLQQWGYGGGENQQF
                     QIVDRGGGKFSLHPRHTGMALDVFFGRADDGTPIVQYPWSGRNNQLWTFQRRDIIPPP
                     AVFCKRGIASNVAPGAAFFPAVAWWYNWSFQPEGTNVGISFVPMIWGRDFLGKAIPAD
                     SKFLLGFNEPNFHEQSNLTAKDAAARWPSVQAQARTTGAAIVSPAVNFCAPAARCNGT
                     DPYQYLKDFFAACQGCKVDYIAVHWYNCDLPSLRDYLEPGGPLQGFEQFGKPIWLTEF
                     SCERDASIAEQERFMRAAIPYLESNPHVFRYSWFSADPIPNARLVNSNGSPTALGRVY
                     INLPRNCP"
     gene            complement(2202..3713)
                     /locus_tag="VF516_06900"
     CDS             complement(2202..3713)
                     /locus_tag="VF516_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8107441.1"
                     /translation="MIILTDSLKSDAPRCAIAFLILTAISCGASAPDLPSRSAGDPLQ
                     FGAPFAGVPDRMDANIYQLNLREYSSSRDIAGARQKLQRIRDLGINVIYLMPIYPLGV
                     MNNDAGSPYSIRDLKSVAGDLGTLSDLRGLVEDAHNLGMSIILDMVVNQTSWDHSWIT
                     EHPSWYVHDNSGGIVSPCPAPNFCFTDVAAMNLDNSEAAAAMIEAMRYWIFAANVDGY
                     RFDWADMAPQAFWTAAIANLKGIGSHRVLLLAEGSNEGTTSGCTTCGQNQPGAHYAQG
                     FDYIFGTNFYWNVMKKIWNTGDPVTNLDKVTAGEYSGASPTQLVARYLSNHDDYNSDG
                     SPFSFLNGGRNAVMAAFVVATYQRGVPFIYNGIEVGNTSPLGFPWNSGNINWTQDLSV
                     YTEMQRILGVRDTSVALRRGTPVSYIDPANTNPDVIAFTKSADTEKVVVLVNVRNGPR
                     SFTIPVEMAGSYVDAYNGASITLIPGARQDLSAFQYLVLTNTNAPVVRSLEYR"
     gene            complement(3870..4436)
                     /locus_tag="VF516_06905"
     CDS             complement(3870..4436)
                     /locus_tag="VF516_06905"
                     /inference="COORDINATES: protein motif:HMM:NF037816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase A-like domain-containing protein"
                     /protein_id="HEX8107442.1"
                     /translation="MRQKSILLQVRDQLNRSTLLLSASALALLTVPAHAQALTKLDRP
                     LQVITLESQEAAGGHTIARHVGKDAAFIKRRVIDQQLDAASTFRDKATAQKVCMAALD
                     AHRAEINAIAFARQSGPANVAFTYDAYRDIGSYLAESNVQDTKKRMKKNKALKTSAHL
                     VTAVVHFNWSVGGRPPHSFFLLTCYPNP"
     gene            complement(4679..5914)
                     /locus_tag="VF516_06910"
     CDS             complement(4679..5914)
                     /locus_tag="VF516_06910"
                     /inference="COORDINATES: protein motif:HMM:NF012409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitinase"
                     /protein_id="HEX8107443.1"
                     /translation="MHRKSTLVSSLQSPVFRFSTFTFVLMIAIMGCHRADAAPSSTRT
                     VGSEDRGGDGNPAQAHPEKPHLRLAQAQAQRGGGDCNAKTWHKGAQYRTNDVVRFTNG
                     QYYVATHDNPGYDPTISTWFWSPTTCSGGGGGNGGGGCNTKTWQKGAQYRTNDVVRFT
                     NGQYYVATHDNPGYDPTISTWFWSAKTCGGSGGGGGGGGGGTRDAGGGAGLAALLSES
                     TFNSMFPGRNPAYTYQGLVQAASSYPAFATSGDTTVRKREVAAFLANVAHETGNLVYI
                     EEINKSDYCSPSPECPCAPGHRYFGRGPLQLSWNYNYCAAGKALHLPLQSQPELVATD
                     AKVAWQTGLWFWMTSTGAGSQTGHDASVSGRFGETIRTINGALECNGKKPETVQSRVQ
                     HYQHFCQLLGVDPGNNLGC"
     gene            7202..>10260
                     /locus_tag="VF516_06915"
     CDS             7202..>10260
                     /locus_tag="VF516_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8107444.1"
                     /translation="MSEVYLARDLRLGRLVAVKLLTTQLPDLDKLLLAEARATARCHH
                     ENIVVIHEVGVQGDQPYMVLEYLEGQSLRQWMIEHAAAAGPRARVSPARAVELMLPVV
                     RALVYAHDQGIVHRDLKPENVLLSRAGTIKVLDFGLAKRVAAPVSDPDSEGGNVHVTK
                     NSRLVGTIPYMSPEQLTLGTVDHYTDVWAVGIMLFELVAGAHPLQGERALLALLMAIA
                     DETIPMPSASELVPELGPLAQIIDRCLIKNPAHRTPSARVLLAELEALAGSRRPVPVS
                     HDGSPFAGLAAFQEADAGRFFGRDRDIEQVVAELRSRPLVAVIGPSGAGKSSLIRAGV
                     IPALKRSGEGWDAHIVRPGREPLAALAALLTHLDSSSGGDASGALSDEAPLRPIVQQL
                     RAEPGTLGARLRARAASKRRRIAVFVDQLEELYTLRAPPAECAAFRACLAAVADDATS
                     PLRVLVSMRSDFLDRLAEDRRLGVEITRGLVLLSPMDRESLREALLRPVEASEHRFEP
                     PALIDRMIDAVAATPGALPLLQFTAARLWERRDRDRRLLTEASYEQLGGVAGALAMHA
                     DAVLNGMSAAQQALARAVLERLITPERTRAPVSVVELHALHRVPALVDEIVQHLAAMR
                     LVVIERGAEGTDPTVELVHESLIDRWPTLVRWLDDHQDDTAMLARLRAAARDWERSGH
                     AAGLLWTSEIAREAGAWRQRYRGELAPIEQRYLAAVLAAAERARRIRRWLFGGILATA
                     VLGVLAATAMAWLAWKQTEAHNEAIMLAGKAAREAMRARDATRMAAMGALAGDPTAQL
                     ALLREIEDVRAPPPEAAQVAKRLLHAGIATVVLRSPEGVFSAAFSPDGRRIVFAWADQ
                     TVRVWNADGSGEPLILRGHDATVWSAVFSPDGRRIVSASSDRTVRVWNADGSGEPLIL
                     RGHDGEVRSAAFSPDGRRIVSASDDQTVRVWNADGSGELLVLRGHSHSTTAAFSPDGR
                     RIVSTWADKTVRVWIADGSGEPLVLRGHDATVGSASFSPDGQRIVSAS"
BASE COUNT         1721 a         3289 c         3409 g         1841 t
ORIGIN      
        1 ccggatcccg atcacgaccg tcgcggcgct cgccccggcc cacacgaaga acgccgcggc
       61 gtgcgagacg ccgagcagga cgagcgcggc gaggaacccg ccgaacagcg gcccgaacag
      121 ccacttctcc gcgcggggcg gcggcgactc cagcgctctg cgctcgaacg cagcgagacg
      181 cggggcagcg ccccccgggt gatcgatcgc cgacccgggc tccggtccgg ccgatcgatc
      241 actgctcatg caacaagctt tgcacacccg gccctggaac gcatcgaccg tccaccgaga
      301 ggggcgtggg tccggtcccg caccttgacc tcgacgttgg ccttgacgcg gaacgtcgag
      361 tttgacggcg acgtggaggt tgcccccacc gtcgacccag aggttgaccg aacgtccagg
      421 tacaaggtcg aggatggagt cgagcccgcg gtcgcgttcg aggtatgcac gaacgagatc
      481 gatctctggt ggcggcgcgg catcgcatat cgagtcgctg gccgcaagcc aggcacgctc
      541 gtgctcgagg gcaaggtcgg cggccggctg ttcgagcagt acgagggccc tgacggcacg
      601 cgcacgcatc aggccggcac gatcaccgcg tgggaccgcc gcgccacctc gcgttcgcca
      661 gccgagccag cgcgtcggat tcgacgaacc gctcgcgggc tcgcgagacg tttcggatcc
      721 ggtcgagccg gtcgatctgc gtcagcaccg cccccttcgc cacgtgggtc tccggaccga
      781 tgcggaacat cctccgcacg aaccccgtag gggcttcccg gtgccacggc ccagcggata
      841 ccagcggctt caggggcagt tccgcggaag attgatgtac actcgaccga gcgcggtggg
      901 agagccgttg ctattgacca gccgggcgtt gggaatagga tcagcgctga accaagagta
      961 gcggaacacg tgcgggttgc tctccagata agggatcgca gctctcatga acctctcctg
     1021 ttcggcgatc gacgcgtctc tctcgcagga gaattcggtc agccagatag gcttgccgaa
     1081 ctgctcgaac ccttggagag ggccgccagg ctccaggtag tctcgcaagg acggaagatc
     1141 gcagttgtac caatgcacgg cgatatagtc gaccttgcag ccctggcacg cagcgaaaaa
     1201 gtctttgagg tactggtagg gatcggtgcc gttgcatcga gccgcgggcg cgcagaagtt
     1261 caccgcgggg gatacgatcg ccgcgccagt tgttctcgct tgagcctgta cgctcggcca
     1321 tctggccgcg gcgtccttcg ccgtgagatt ggactgctca tggaagttcg gttcgttgaa
     1381 gccgagcagg aacttggaat ctgcggggat ggccttcccc aagaagtcgc gcccccagat
     1441 catgggaacg aacgagatac ccacgttggt gccctcgggc tgaaaactcc aattatacca
     1501 ccaggcgacc gccggaaaaa atgcggcacc aggcgccacg ttggaggcga tcccccgttt
     1561 gcagaacacc gctggagggg gaatgatgtc cctgcgctgg aaggtccaga gctggttgtt
     1621 ccggcccgac caggggtact ggacgatggg tgtcccgtcg tcggcgcggc cgaagaatac
     1681 atcgagcgcc atacccgtgt ggcgcgggtg caggctgaac ttcccgccgc cccgatcgac
     1741 gatctgaaat tgctggttct cgcccccgcc gtatccccac tgctgcagag gagcgttttc
     1801 ctgggtgctg gcattgcgga tatcgagaga cttcttgctg ttgacgttga cgagcttgta
     1861 gaaaccacca cctagctggg ttacctcgaa gatctgcgcc tgggtgtcgt tgcagctcca
     1921 ctcttggagc ggggcgccat cggcggtatt cgagccgggg acctccaggc acttgccact
     1981 gacggcagtg tggatggtgt aattgccatc ggtgatcgcg aatgcgttgc ggctctccgc
     2041 gagcagggtc atcgccaacg cgagcatata caggaaacgg gcatgcatcg tatgttcctt
     2101 ggtcgttgga ccacatccac ggtggtacgt caggtgattg gtgccaacta tttgcggctc
     2161 tttacgtcgg ggctcgacgg gaggaaaacg ctgcccaggg ctcaccgata ctccagtgac
     2221 ctcacaaccg gcgcgttcgt gttggtcagc accagatact gaaacgctga aaggtcttgc
     2281 ctggcgccgg ggatcagcgt gatgctggcg ccgttatacg catcgacata actccccgcc
     2341 atttctactg gaatcgtgaa ggatcggggg ccgttcctga cattcaccag caccacgacc
     2401 ttctccgtat cggctgactt cgtgaatgcg atcacatccg gattggtatt ggccgggtcg
     2461 atgtaggaaa ccggtgttcc gcgcctcagc gcgacgctcg tgtctcgcac accgagaatc
     2521 ctttgcatct ccgtatagac cgacagatct tgtgtccagt tgatgttccc tgaattccac
     2581 gggaagccga gtgggctggt attcccgact tcaatgccat tgtagatgaa cgggacgccc
     2641 cgctgataag tagcgaccac aaatgcggcc atcaccgcat ttcgaccgcc attcaagaac
     2701 gaaaacggcg agccatctga attgtaatca tcatgattgc ttagataccg tgcaacgagt
     2761 tgcgtcgggc ttgctccgga atattccccg gcggttacct tgtcgaggtt agtcactgga
     2821 tcgccggtgt tccagatctt cttcatcaca ttccagtaga agttcgtacc gaagatatag
     2881 tcgaatccct gggcataatg cgcccccggc tgattctggc cgcacgtggt gcaaccagac
     2941 gtggtgccct cgttggaacc ctcagcgagc agcaagacgc gatgagaccc gatccccttc
     3001 aagttggcga tcgcagcggt ccagaaggcc tgcggcgcca tatcggccca gtcaaaacga
     3061 tatccgtcca cattggcagc gaatatccaa tatcgcatag cctcgatcat cgcggcggcg
     3121 gcctcgctgt tgtccagatt cattgcagct acatccgtga agcaaaagtt aggcgctgga
     3181 cacggagaca cgataccgcc gctgttatca tgtacatacc aggaaggatg ctctgtgatc
     3241 catgagtgat cccaggaggt ctggttcacc accatgtcta gaatgatcga catgccgagg
     3301 ttatgggcgt cctccacgag gcctcgcaag tccgataacg tgccgagatc accagcgacg
     3361 cttttgagat ctcggatgct gtaaggcgag cctgcgtcgt tgttcatgac gcccagcggg
     3421 tagatcggca tcaggtagat cacgttgatg cccagatctc tgatccgctg gagtttctgt
     3481 ctggcgcccg caatatctcg cgatgaactg tattcccgca agttgagctg atagatattg
     3541 gcatccatcc gatcagggac ccccgcaaaa ggggccccga actggagcgg gtccccggct
     3601 gacctcgaag gcaggtccgg cgccgacgcg ccgcaggaga tcgcggtcag tatgagaaat
     3661 gcgattgcac atcggggagc gtccgatttc aggctgtcgg tgagaatgat catgatgtac
     3721 gttccttctg aggtctacat gagtgatcgg cgagccaaga gtgcggcact gggttttcgt
     3781 gggcgagggg acgggtgcgc gccggcgaca cgcggccagc gcgcactcgt cccctcatcg
     3841 gtcatcgctc cgcccccagg ccgaggccgc tacgggttgg ggtagcaggt gagcaggaag
     3901 aagctgtgcg gcgggcggcc gcccacgctc cagttgaagt gcaccacggc ggtgaccaag
     3961 tgggcgctgg tcttgagcgc cttgttcttc ttcatccgct tcttggtgtc ctgcacattg
     4021 ctctcggcga ggtaggagcc gatgtccctg tacgcgtcgt aggtgaaggc cacgttggct
     4081 gggccgctct ggcgcgcgaa ggctatggcg ttgatctcgg cgcggtgggc gtcgagcgct
     4141 gccatgcaaa ctttctgggc ggtggccttg tcgcggaagg tggaggcagc gtcgagctgc
     4201 tggtcgatca cgcgccgctt gatgaaggcc gcgtccttgc cgacgtggcg ggcgatggtg
     4261 tggccgcccg cggcctcctg gctctcgagc gtgatcactt gcagcggcct gtcgagcttc
     4321 gtgagcgcct gggcatgggc cggcacggtg agcagcgcaa gggcggaggc ggagagcaac
     4381 agtgtggagc ggttcagctg atcgcgcacc tggagcagaa ttgatttctg acgcatattc
     4441 cttcatcctg tattcgaggt tgagctcggc atcgagcggt cgtgtgcctg cgcgaattca
     4501 ggatccgatg accaccaccg tgcgctggac tcggcggctc cgatgtgccg cgcgaggtac
     4561 acatcggcca tttcacctcg gcccaggtgg ccagtgagac agtaatcgcc gagctctgcc
     4621 agcatccggg gtcctgagcg cccccgcgtg acgatccaac ccacgctcgc ccggtacgtc
     4681 agcagccgag attgttgccg ggatcgacac cgagcagctg gcagaagtgc tggtagtgct
     4741 gcacgcggga ctgcaccgtt tcgggcttct ttccgttgca ctcgagggcg ccgttgatcg
     4801 tccggatcgt ctcaccgaac cgaccgctca cgctcgcgtc atggcccgtc tgcgacccgg
     4861 cgccggtcga cgtcatccag aaccacaggc cggtctgcca cgccactttg gcatcggtgg
     4921 ccacgagctc gggctgtgac tgcagcggga ggtggagcgc cttaccggcg gcgcagtagt
     4981 tgtagttcca gctcagctgc agcggcccac ggccaaagta tcggtgtccg ggcgcacacg
     5041 ggcactcggg ggagggcgag cagtagtcgg acttgttgat ctcctcgatg tagacgaggt
     5101 tgccggtctc gtgggcgacg ttggcgagga aggccgcgac ctcgcgcttg cgcacggtgg
     5161 tgtcgccgct ggtggcgaaa gcggggtagc tcgacgcggc ctggacgagg ccctggtagg
     5221 tgtaggccgg attgcggccg gggaacatgg agttgaatgt cgactcactg aggagcgccg
     5281 ccaggccagc gccaccgccg gcatcacggg tgccgccgcc gccgccacca ccgccgccgc
     5341 cggagccgcc gcaggtcttt gcgctccaga accaggtgct gatcgtcgga tcgtagccag
     5401 ggttgtcgtg tgtcgcgacg tagtactgtc cgttggtgaa ccgtacgacg tcgttggtgc
     5461 ggtactgcgc tcccttttgc cacgtcttgg tgttgcaacc accgccgccg ttgccgccgc
     5521 cgccgccgct gcaggtcgtg gggctccaga accaggtgct gatcgtcgga tcgtagccag
     5581 ggttgtcgtg cgtcgcgacg tagtactgtc cgttggtgaa ccgtacgacg tcgttggtgc
     5641 ggtactgcgc tcccttgtgc cacgtcttgg cgttgcaatc gccaccgccc cgctgagcct
     5701 gcgcttgggc cagtcggagg tggggctttt ccgggtgagc ttgcgcaggg ttaccgtcgc
     5761 caccacggtc ttcggagccg accgtgcgcg tactgctggg tgccgcgtcg gcgcgatgac
     5821 agcccatgat ggcgatcatg agaacgaagg tgaacgtgga gaaccgaaag accggcgact
     5881 gcagagacga aacgagagtg gatttcctgt gcatgttctt tcatcctttg tgtacggtat
     5941 tgtgcatggc gcgcgcggcc gcgagtagtg cgccagattg atcgcactcc gcgacatgcc
     6001 tgaatcctgg ttactatgag ctacgttccg gctccgtgcg ttgcgaagcc tggagaagac
     6061 cgatggcctg aatcagcaca ctacgttgtt ccatgtcggc ggtcgccacg atctcatcgg
     6121 tcatcgcaac tcggcgccgg agtggaccgg cccgccgagc acctcgtcct tgggggaggg
     6181 tcaacctgag ccgtaacacc ttgctggcac cggtgacggc gacatcgcca cgatcgcatc
     6241 agacatcgcg aagccctagg gagcgccgcg gactcagtca tcgagctgca cgctggcacg
     6301 ctcctggtga atggtgagct ccgcctcgcg aacaaccgcc acctcgacgg ctaccgggta
     6361 ctcgtggttc acaatatctc tgggagagca agacaggggc cagcgttcgc gcctggagtg
     6421 cgcgtgcaga tgcgagcgtc gagtgctcga tggccggctc gtgatcagga aatccagtca
     6481 ttttcggaac gctacagagt ccgcagaatc agacagtgtc cgctggcgcg gatcctgaag
     6541 tccagtttag cggaccgcga tagctgccac gacttgcctg ccacagcctg cctgccatga
     6601 cttgccaggc gggatgccat gattgtagca gcgcacgctc gcgacagcga tgcctctggg
     6661 cgttcgttga tcatcggaac ctcgcaacat gagctgcgat cacctcgggc cagcgctgga
     6721 agacattgga tttttacaat gtacgttcat gcgtataacg ctggtaatgt cgcgcatccg
     6781 gtgttcggtc cgatcaccca cctggtgcga accaggtgta tcctggctca ggcccatagc
     6841 atcgtgctat ctcgtcgagc tctgatcatt gggcactacg aggtgggctt tccagtgaga
     6901 tgaaaagaac gacctgaggt cacagtggaa gactctactt attattcgga gtctcctcga
     6961 acactacgcg gccatacatc agatacgtgg cactccgggg caagtaacag cgcacttttg
     7021 tcgtgcggat cggtctctgg cgcaccagca cctggtggcc tatcttgcgc tttacgtgat
     7081 gcccaccatg cctgccacgg tccatgcagg aggatcggaa gtcggatcgc catcggtcca
     7141 gttcccccca ggcagccgga ttggtcagta cgagctcatt cgcctgctgg gctgcggcgg
     7201 catgagtgag gtgtatctgg cccgcgacct ccgcctgggc cgcctggtcg cggtcaagct
     7261 gttgaccacg cagctgcccg acctcgacaa gctgctcctg gccgaggccc gcgccaccgc
     7321 gcgctgccat cacgagaaca tcgtcgtgat tcacgaggtc ggcgtgcagg gggaccagcc
     7381 ctacatggtg ctcgagtacc tcgagggaca gagcctgcga cagtggatga tcgagcatgc
     7441 ggccgcagcg ggaccgcgcg cccgggtatc gccggcccgt gccgtggagc tgatgctccc
     7501 cgtcgtgcgc gcgctggtgt acgcacatga tcagggcatc gtccatcgcg acctcaagcc
     7561 cgagaacgtc ctgctcagcc gggccgggac catcaaggtg ctcgactttg gcctcgccaa
     7621 gcgggtggca gcgccggtat ccgatccgga ctccgagggt ggcaacgtgc acgtcaccaa
     7681 gaacagcagg ttggttggca cgatccctta catgtcgccc gagcagttga ccctcggtac
     7741 agtcgaccac tacaccgatg tgtgggccgt gggcatcatg ctgttcgagc tggtcgcggg
     7801 tgcgcatccg ctgcagggtg agcgggcgtt attggccctg ctgatggcca tcgccgacga
     7861 gaccatcccg atgccgagcg cgagcgagct cgttcccgag cttggcccgc tcgcccagat
     7921 catcgatcgc tgcctgatca agaacccggc gcatcgcacc cccagcgcac gcgtgctcct
     7981 ggccgagctc gaggcgctgg cgggaagccg ccgccccgtg ccggtgagcc atgacgggag
     8041 cccgtttgcc ggactggccg cgttccagga ggccgacgcc ggccggttct tcggccgcga
     8101 ccgcgacatc gaacaggtgg tcgcggagct gcgcagccga ccgctggtgg ccgtcatcgg
     8161 cccctcgggc gccggcaagt cgtcgctgat ccgcgcaggc gtcatcccgg cgctcaagcg
     8221 ctcgggcgag ggctgggacg cccacatcgt acgtccgggc cgcgaacccc tggccgcctt
     8281 ggccgccctc ctcacccacc tggacagctc ctccggcggt gacgcctccg gggcgctctc
     8341 ggacgaagcg ccgctccgtc cgatcgtcca gcagctgcgc gccgagcccg gtacgctggg
     8401 cgctcggctg cgcgcgcgcg ccgcgagcaa gcgccgccgg atcgccgtct tcgtcgacca
     8461 gctcgaggag ctctacaccc tccgcgcgcc ccccgccgag tgcgccgcct ttcgcgcctg
     8521 cctggctgcg gtggccgacg acgcgacctc gcccctgcgc gtgctggtgt cgatgcgctc
     8581 cgacttcctg gaccgcctgg ccgaggaccg ccggctgggc gtcgagatca cccgcgggct
     8641 ggtcctgctg tcgcccatgg accgcgagag cctgcgcgag gcgctgctcc ggccggtcga
     8701 ggccagcgag caccgcttcg aaccgcccgc gctgatcgac cgcatgatcg atgccgtcgc
     8761 ggccacgccc ggcgcgttgc ccctcttgca gttcaccgcc gcgcggctgt gggagcgccg
     8821 cgaccgcgac cggcgcctgc tgaccgaggc cagctacgag cagctcggcg gcgtcgccgg
     8881 cgccctggcc atgcatgccg acgccgtgct gaacggcatg tccgcggccc agcaggcgct
     8941 cgcgcgcgcc gtgctcgagc gcctgatcac tcccgagcgc acccgggccc cggtcagcgt
     9001 ggtagagctg cacgcgctgc atcgcgtgcc cgcactcgtc gacgagatcg tccagcacct
     9061 ggcggcgatg cgcctggtcg tcatcgagcg cggcgccgag ggtaccgacc ccaccgtgga
     9121 gctggtgcac gagtcgctca tcgatcgctg gcccaccctg gtgcgctggc tcgacgacca
     9181 ccaggacgac acggcgatgc tcgcccggct gcgcgcggca gccagggact gggagcgcag
     9241 cggccacgcc gcggggctgc tgtggaccag cgagatcgcg cgcgaggccg gcgcctggcg
     9301 gcagcgctac cggggtgagc tggcgccgat cgagcagcgc tacctcgcgg ccgtgctcgc
     9361 cgcggccgaa cgcgcgcgtc ggatacggcg gtggctgttc ggcgggatct tggcgacggc
     9421 cgtgctaggc gtgctggccg cgaccgccat ggcctggctg gcctggaagc agaccgaggc
     9481 ccataacgag gcgatcatgc tcgctggcaa agcggctcgg gaggcgatgc gagcccggga
     9541 tgccacccgg atggccgcga tgggcgcact ggccggagat cccacggccc agctcgcgct
     9601 gctgcgcgag atcgaggatg ttcgcgcgcc cccgccggag gcagcccagg tggccaaacg
     9661 cctgctgcat gccggcatcg cgaccgtcgt cttgaggagc cccgagggcg tcttctcggc
     9721 ggcgttcagc ccggacggcc ggcgcatcgt gttcgcgtgg gcggaccaga cggtgcgggt
     9781 gtggaacgcc gatggcagcg gggagcccct catcctgcgc ggccacgacg ccacggtctg
     9841 gtcggcggtg ttcagtccgg acggccggcg catcgtatcc gcgtcctctg accggacggt
     9901 gcgggtgtgg aacgccgatg gcagcgggga gcccctcatc ctgcgcggcc acgacggcga
     9961 ggtcaggtcg gcggcgttca gcccagacgg ccggcgcatc gtgtccgcgt ccgacgacca
    10021 gacggtgcgg gtgtggaacg ccgatggcag cggggagctc ctggtcctgc gcggccacag
    10081 tcacagcacc acggcggcgt tcagcccgga cggccggcgc atcgtgtcca cgtgggcgga
    10141 caagacggtg cgggtgtgga tcgccgatgg cagcggggag cccctggtcc tgcgcggcca
    10201 cgacgccacg gtcgggtcgg cgtcgttcag cccggacggt cagcgcatcg tgtccgcgtc
//
LOCUS       DASVOR010000337         4325 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_119889,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000337 DASVOR010000000
VERSION     DASVOR010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4325)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4325)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4325
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119889"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..1433
                     /locus_tag="VF516_06920"
     CDS             42..1433
                     /locus_tag="VF516_06920"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8107445.1"
                     /translation="MPLPFVDRRAELGRLCRALTARTSSLVCVYGRRRVGKSRLVTTA
                     LEGLAAVYYLADERDEHLQRHSLAREIERLIPGFARVEYPDWDALLDRWAEAAPAGAV
                     LAIDELPYLVRAAPALPSILQRRIDRGPGRQHWVICGSSQRMMHGIVLDAAAPLYGRA
                     REILKVEPLGFPYLSEALRLRRAEDAVEAYATWGGIPRYWVLADGAASLQAAQRDLIL
                     DPLGVLHAEPQRLLLDEVSETMQAHSILALIGQGCHRLSQIAGRLRRPATSLSRPLAL
                     LVDIGLVKRELPFGEHPRRSKRTRYRIADPFLRYWYRFVDPNRSLLEARHLDVQTQTA
                     WLSHLGESWEEIVRASIPDVSIDTARWQPAQPWWGRAGDGPDAEIDVVAAHRDDRNRL
                     LVGEVKLSLDAADVPRLLHDLEAKARACDWARNKQLTLRLWTLRWQARSRRPPAVIDA
                     RELQARWREASAA"
     gene            complement(2226..2678)
                     /locus_tag="VF516_06925"
     CDS             complement(2226..2678)
                     /locus_tag="VF516_06925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107446.1"
                     /translation="MHGATVFGDIEKSSPRARLANSLGYVPAKVQFGHHVPSDFFGYF
                     VSMLLVRIDSKSMISPVIDTRPVETTASRRHIVASRCHDADTVLPHHRSDLSGKISGA
                     QRRRVCQEHRVLIGRGSFGDCTEDEPKEAFELFGGLRADCVLADVYVG"
     gene            complement(2762..3007)
                     /locus_tag="VF516_06930"
     CDS             complement(2762..3007)
                     /locus_tag="VF516_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107447.1"
                     /translation="MVDHHGDVLVMSPVRQLVDTDLREAIESIGTTVAGNDALDDRSG
                     AYPSGIHPQNLEESLFSYGSIYTTRRRPAATMCLAQL"
     gene            2996..3729
                     /locus_tag="VF516_06935"
                     /pseudo
     CDS             2996..3729
                     /locus_tag="VF516_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013753.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4076..>4325)
                     /locus_tag="VF516_06940"
     CDS             complement(4076..>4325)
                     /locus_tag="VF516_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107448.1"
                     /translation="VALWLVNLELFARIVFPWLLAVPQPVQVFLHAVCFGLPLGLRYA
                     AAARDAPQAPQLPVAIAYRRAASFRRARRTLRRARRPS"
BASE COUNT          738 a         1438 c         1448 g          701 t
ORIGIN      
        1 aattatgctt cggaaacata atgtcgcggt agcataatgt tgtgccgctc cccttcgtcg
       61 accgccgcgc cgaactgggt cgactgtgcc gcgcgttgac ggcccggacc agctcactgg
      121 tgtgcgtcta cggccggcgc cgggtgggca agtcgcggct cgtcaccacg gcgctcgaag
      181 ggctcgcggc ggtctactac ctggccgacg agcgagacga gcatctgcag cgccactcgc
      241 tcgcccgcga gatcgagcgc ctgatccccg gcttcgcgcg cgtcgagtac cccgactggg
      301 acgcgctgct cgatcgctgg gccgaggccg cgccggcagg tgccgtgctc gccatcgacg
      361 agctgccgta ccttgtccgt gccgcgcccg cgcttcccag catcctccag cgccggatcg
      421 accgtggccc cggtcgccag cactgggtga tctgcggctc ctcgcagcgc atgatgcacg
      481 gcatcgtcct cgacgccgcg gcgccgctct acggacgcgc ccgcgagatc ctcaaggtcg
      541 agccgctggg gttcccctat ctctccgagg cgctccgtct tcgccgcgcg gaggacgccg
      601 tcgaggcgta tgcgacctgg ggcggcatcc cgcgctactg ggtgctcgcc gacggcgccg
      661 catcgcttca ggccgcacag cgcgatctca tcctcgaccc cctcggcgtc ctgcacgccg
      721 agccccagcg cctgctgctc gacgaggtca gcgagaccat gcaggcgcac tcgatcctcg
      781 cgctgatcgg ccagggctgt caccggctct cgcagatagc cggccgcctc cgccgcccgg
      841 ccaccagcct gagccgccca ctcgcgctgc tcgtcgacat cggcctcgtc aagcgcgagc
      901 tgccgttcgg cgagcatccg cgccgcagca agcgcacccg ctaccggatc gccgatccgt
      961 tcctgcgcta ctggtaccgc ttcgtcgacc ccaaccgctc gctgctcgaa gctcgccacc
     1021 tcgatgtcca gacccagacc gcgtggctct cgcacctcgg cgagagctgg gaggagatcg
     1081 tccgggccag catccccgat gtctcgatcg acactgcgcg ctggcagccg gcgcagccct
     1141 ggtggggacg ggcgggcgac ggcccggacg ccgagatcga cgtcgtggcg gcccaccgcg
     1201 acgaccgcaa tcgcctcctg gtcggcgagg tcaagctctc gctcgacgcc gccgacgtgc
     1261 cccgcttgct ccacgatctc gaagccaagg cccgcgcgtg cgactgggcg cgcaacaagc
     1321 agctcacgct ccggctgtgg acgctgcgct ggcaggcccg cagccgccga ccgccggcgg
     1381 tgatcgacgc tcgagagctg caggccaggt ggcgggaggc gagtgcggcg tgatgccagt
     1441 gctgatcacg cgagccagga gatcggttcg cgggggggga tcgcacgtcg tccggcagca
     1501 gcgggatgaa caacatccgg accgctggac cgggcggggg ggccccatgt ctcgggagcg
     1561 atccctatcg gcctttcctt gcgacgccgg atccgctctg gaaaactgag acctgtcagc
     1621 cccctccggt aaggttcgtt gcccatgcta tcggaaaccg acaagttgtt gcgcgatccc
     1681 ggttcggagc tggtgttcgg cttggtagcc ccggtcggag ccgacctcga tgcactggag
     1741 gaggatctct cgaatcaact ccggcagtat ggggtacacg ccgaacccga tccgcctgag
     1801 ccggcttctg aagggcgctc gcaacctcgg catcgagctg aaggagaccg gcgaattcga
     1861 gcggctcatg agcttcatgg atggcggaga ccgaattcgt gagaagtcgg gctccggcga
     1921 gatcctcgcg ctgtgggcga tggcatcgat caagaacaag cccccgccag aaagtcccgt
     1981 gcgtcgaagg accgcttaca tcctccgatc gatcaagcac ccggatgaag ctcgcgcgtt
     2041 gcgggccgta tacggaggcg gattcttcct gatcggcctg tcagcctcga cagcacagag
     2101 gaagtggtcg ctgacacaga agggcctcac cgaggatcag gcgaagacgg tcatcgagcg
     2161 cgatgagagt gaagagaggg tgctgggcca caagctgggt cagcagacgc gcgaagccct
     2221 tcgagctagc cgacgtatac atccgccagc acgcaatccg cacgaagacc accgaacaac
     2281 tcgaacgctt ccttcggctc gtcttcggtg cagtcgccga aactcccacg cccgatgagc
     2341 acgcgatgtt cctggcatac gcgtcttctt tgcgctccgc tgatctttcc cgacaggtcg
     2401 gagcggtggt gtggaaggac ggtgtcggcg tcgtggcaac gggatgcaac gatgtgcctg
     2461 cggctggcgg tggtctctac tggccgggtg tcgatgacag gcgagatcat gctcttggag
     2521 tcgatgcgaa cgagcaacat agagacgaaa tagccgaaga aatcgctcgg cacgtggtgg
     2581 ccgaattgaa ccttggcggg gacatatccg agagaattcg ccaggcgtgc acgcggactc
     2641 gacttctcga tatcaccgaa tacggtcgcc ccgtgcatgc ggagatggat tcgttgctca
     2701 gctgtgggcg tgcagggatc ccgacggtgg gggcgacgct gttctcgacg acctttccat
     2761 gtcacaactg cgccaaacac atcgtcgccg ccggcctgcg gcgtgtcgtg tatatcgagc
     2821 cgtacgaaaa gaggctctcc tctaggtttt gtgggtggat cccggatgga tatgcaccgg
     2881 atcgatcatc cagcgcgtcg ttgcccgcca cggtcgtccc gatgctctcg atggcctcac
     2941 gcagatcggt gtcgacgagc tgtcttaccg gcgacatcac gagtacgtca ccgtggtggt
     3001 cgaccaccga cgcggccacg ttgtgtgggc tcagccgggg aagaacgccg acacgctcaa
     3061 ggcgttcttc gacgagctcg gaccggagcg ttgtgccaag ctggaggcgg tcaccatcga
     3121 catgtccgcc gcgtacatca aggcggtcac cgaatgctcg ccgcaggcca agctcatctt
     3181 gaccggttcc atgtcctgcg gctggcacag gatgccgtcg atgaggtccg ccgagacgaa
     3241 gttcgtgccg ccgcgaccgt cgagcaaggc aagcagctca aggggacacg ctggagcttg
     3301 ctcaagagct tctggaacct gtcgctgttc gattcggccc ggctctcgca gctgcaacgg
     3361 gacaacaagc gcctctaccg agcctacttg ctcaaggatg ccctggtccg cgttctcgac
     3421 tgccgagatg agtggctcgc ccgggtcaag ctcgcccagt ggatccgctg ggcgcgacgc
     3481 tcgcggctcg aacccttcca gcgcatcgcg gtcacaatcc gcgagcacgc ggagggcatc
     3541 atcgcctacg tcacctcgga actctccaac ggccgaaccg agggcctcaa cggcaaggct
     3601 cgtacgatca cgcgccgcgc ctatggcttt cacagtgccc atgggctcat cgccctgctc
     3661 aagctctgct gttcaggcat tcatctcgat ccggtgcata tccatccggg atccacccac
     3721 aaaacctaga ggagagcctc aatatgggcc acggcacgca gcccgcggct cggccggggt
     3781 tgggagtaca cagcctcaga tcggcggtcg agatcgacgg aggatgcggt gccatggcga
     3841 tcatggcggt ccgagcgccc aggaagaaac cggcatggac gaacgggtgg tcgcggagac
     3901 tacagctcca acgagccggc gccgctgggg cgctgcgcca tgctgtgggg cgggggcgct
     3961 ggaaggtggc gtttggttgg cgtgtgccta tcggctaccc agacgcacgt cgtgaagcgg
     4021 cttcgcgctg cccaggtcgg ggacgcgcga gtgggtgcgc ccgttcacga gctcgtcagg
     4081 atggccggcg ggctcggcga agcgtacggc gggctcggcg gaacgaggcg gcgcggcgat
     4141 aggcgatcgc gaccggcagc tgcggggcct ggggcgcgtc gcgtgcggcg gcggcgtagc
     4201 gcaggcccag ggggaggccg aagcacaccg cgtggaggaa gacctggacc ggctggggca
     4261 cggcgagcag ccaggggaac acgatgcggg cgaacagctc caggttgacc agccacagcg
     4321 cgacg
//
LOCUS       DASVOR010000338         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_265906,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000338 DASVOR010000000
VERSION     DASVOR010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265906"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF516_06945"
     CDS             complement(<1..180)
                     /locus_tag="VF516_06945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107449.1"
                     /translation="MKYTVVMVPSATRARVLVSHGTDELLRAILPPPSAIRYQEAALA
                     FLQGLSLWLDEKLRVV"
     gene            888..1052
                     /locus_tag="VF516_06950"
     CDS             888..1052
                     /locus_tag="VF516_06950"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEX8107450.1"
                     /translation="MQRLPIAYTILGSCRLAGVDPREYLADVLPQLTGRIRLMDLPAL
                     LPSRWGAHRR"
BASE COUNT          234 a          607 c          509 g          272 t
ORIGIN      
        1 tacaacgcgg agcttctcgt cgagccacag cgacaatccc tgcagaaacg ccaacgctgc
       61 ttcctggtaa cgtatcgccg agggtggcgg caagatcgcc cgcaagagct cgtcggtccc
      121 gtggctcacg agcacgcggg cccgtgtcgc cgacgggacc atcacgaccg tgtatttcat
      181 ctgcgtccgc ttcgccgtcg gccgcggcct tgctcgtcgt gagcatcggc gggtcatcgg
      241 gcgcggacca ttcagcacga ggcgcaactt tttggcgact cacatccgtc accgcctgtg
      301 cttccgctcg cttccgcgtc accagcgccc gctgatgctc ggcgtggtct cgtcggcctt
      361 cttcgctccg ctgatgcttg gcattcgcga ggcggcgcag ggcggactgc tcaagctcgc
      421 gacagacgtc cttgcagtag ctttggcctc ggtagtcagg gcgacagacg acgaacccct
      481 tcgcgcagta gctgcaggtc aactcgaaga caacgagctc gtccatccat cgcggacggg
      541 cgagaactgg acaactgctt cacggactgc gacagtacga gagtactggc gaggtccatc
      601 caccgcttgt acatcgaggg ctcaggcgag aatctgagcc ctcactcttt ccatccgacg
      661 gtccgaatta tcggagccga ccctacagca ccgccacttc ctgagcccag gcgtggtccc
      721 cgggcccccc tgatctcctg gtccgatccc ctggtccccg tccctggtcc cctggtcccc
      781 ggtgattccg gtggaagtgg tcccctgcga ggccggtggt gacacggccc gtgatgtgat
      841 gatgcgcacg ccgcgcttgt cgcaccacgg cgggaccgat ggggtccgtg cagcggttac
      901 cgatcgcata cacgatcctc gggtcctgcc gccttgccgg cgtcgacccg cgcgagtacc
      961 tcgccgacgt cctgccgcag ctgaccggcc gcatccgcct catggacctg ccggcgctgc
     1021 tgccgtcgcg ctggggtgcg caccggcggt gacggcggtg accgtcgcga ccgtggctgc
     1081 cgcgcaggac tgatctggcg ctgccacagg ctggcgccgc gctcaagcac ggcccgggat
     1141 ttcggctgga ttcggccgga cgctcacgct cggccagatc ctccacgact accgccgcca
     1201 cgtccttgcc cggctcgccc tcgagcgccg ggccgaccca ttctgagctg acctacccgc
     1261 gagccgaggc cgctgccacg gcgcgctcca ccgtccgctc cggacgcggc tcactgccaa
     1321 cgtgtctgca acgagcccac ctcagccaag ccggtcactg ccagccgatc tgcaaccagg
     1381 ctgcctcagc cccgccgacc tgctgccaac cgatctgcaa cgagccctgt ctctgggcgt
     1441 gcaacgcaac atggcgcctc aggccagcgg cgcgcccacg accggcctgg caatcgaccg
     1501 acgctcgcgg tcggtctggc gcccaggcgc ccggtgcgct ttcgctcggc gcgcttgcgg
     1561 tcggtgcgct cggcccggcg caggtggggc gcctcgcggt cagcggcgac gccctgccag
     1621 ag
//
LOCUS       DASVOR010000339         4304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_411602,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000339 DASVOR010000000
VERSION     DASVOR010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4304
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411602"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..3013)
                     /locus_tag="VF516_06955"
     CDS             complement(23..3013)
                     /locus_tag="VF516_06955"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX8107451.1"
                     /translation="MIFTVACATGPSTSAPPGSGNPGDPGNPNPGAPPSAVSTDQLAT
                     SFGVAILASEPSGAPRLLRTIVPRPSAAGLTAAAAALDHVATLSALWVRNTQPMALAD
                     NGVQQLRNGATMIKLVQNVDGVPVHGGEIHVLLGADGGLAAISGTLLPAVAAPSFASS
                     ATDALTHALDQHFGPARPQMSITETPASGDTGGWQPLQVASTSALQVDEARARRTLAQ
                     VGDQLVPAYEVEVQGNAAPDPLADPSIPTFSAHSYLVRDADGKILDDENLIANDAFVY
                     RVYMETTGNRRPLDGALASFAPHPTGVPDSSAPSLLPANLVVMDSFNKNFDPWLPTNA
                     TTTSGNNAEAFADLDGNRSFTPGDFRPVVKSGRVLNYVYDHTLEPLANTTQSSAAAVN
                     TFFLVNWMHDWYYDSGFTEATANAQLDNFGRGGVANDPLRIDAQAGANVGSRNNANMS
                     TPADGARPTMNMFLWTAGTLTGLSGPTGTPRTEPMAAGPRNFDLVGDLAFATDGTAPN
                     DDACQTVTSNVAGKIAVVNFSGITGCGSLNVVNNVKAAGAIGIVLVDPALDDPRAFAG
                     SAAANIPSVAMGKTDGAALEAALAAGPVTVEITSAVQGPERDGDLDNTVIAHEWGHYL
                     HHRLENCGGAGSQQCAGMSEGWGDFNSLMMMAREGDNRDGVYAEGYYALADGVTPNFA
                     YFGIRRFPYSRDRTKNDLSFRHISNGVALPTTTPGFPGDGSNNSEVHNTGEIWASMMW
                     EVFNVVADAHGVEVARRRMADYIVAGFLMSPRNPSFTEQRDAILVAASAMDSDDMLVM
                     AGAFAGRGIGSCAISPDKASAANAGVVESGTLAAKLAVGGLSVDDNGPKGDHDHILDP
                     GESGTLSLTLANNGIIAAENVTVTATSPGAGVKFGAPLVIAAQPKFSSSTLSIPISLA
                     ANAPLNTMITINVHVAGQFTCDRNGIDATLTVLTGANPMMVNASRLTSEDAATDQSPV
                     VSTIVAPSTSLRAFDAACSASAAQ"
     gene            complement(3466..>4304)
                     /locus_tag="VF516_06960"
     CDS             complement(3466..>4304)
                     /locus_tag="VF516_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEX8107452.1"
                     /translation="WLRHRVPAQVVEVDEPLAALERLRSHLDVLEARLARQEHDLRGA
                     SEDVARGIDVQLRRAAGQIRRLNQHLDGIQFGSVRGIRIELRRIDRMEQILRALRLGE
                     AQELLFLSAIPIEEALDEIFRRYGGGRAGGERLLDYREYLELAVQIRRQAAADWETAS
                     PTRLSTGEAIGVGAAVMMVVLTEWERDANLLRARRTTGSLRFLFLDEANRLSRDNLGL
                     LFDLCKTLDLQLLIAAPEVARAEGNTTYRLVRHVTDDGREEVIVSGRRAAGAAQPPSE
                     AS"
BASE COUNT          587 a         1453 c         1605 g          659 t
ORIGIN      
        1 agccgcgccg gcgatcaggt cgctactgcg ccgcgctcgc gctgcacgcc gcgtcgaacg
       61 cgcgcaggct cgtggacggg gccacgatgg tcgagaccac ggggctctga tcggtcgccg
      121 cgtcctcgga ggtcaggcgc gaggcgttga ccatcatcgg gttggcgccg gtcaggaccg
      181 tgagcgtggc atcgatcccg ttccggtcgc acgtgaactg gccggcgacg tggacgttga
      241 tcgtgatcat ggtgttgagc ggcgcgttcg cggccagcga gatcgggatc gacagcgtcg
      301 agctcgagaa cttcggctgc gcggcgatca ccagcggcgc gccgaacttg acaccggcgc
      361 ccgggctcgt cgccgtgacg gtcacgttct ccgcggcgat gatgccgttg ttggccaggg
      421 tcaagctcag cgtccccgac tcgccggggt ctaggatgtg gtcgtggtcg cccttggggc
      481 cattgtcgtc gacgctgagc ccacccaccg cgagcttggc ggccagcgtg ccgctctcga
      541 ccacgccggc gttggccgcc gaggccttgt cgggcgagat cgcgcacgag ccgatgccgc
      601 ggccggcgaa cgcgccggcc atcaccagca tgtcgtcgct gtccatcgcg ctcgcggcga
      661 ccaggatcgc atcgcgctgc tcggtgaacg acgggttgcg cggcgacatc aggaagccgg
      721 ccacgatgta gtcggccatc cggcggcggg cgacctcgac gccgtgcgcg tcggcgacca
      781 cgttgaatac ctcccacatc atgctcgccc agatctcgcc ggtgttgtgg acctcgctgt
      841 tgttggagcc gtcgccgggg aagcctggcg tcgtggtcgg gagcgcgacg ccgttgctga
      901 tgtggcggaa gctgaggtcg ttcttggtcc ggtcgcggct gtacgggaac ctccggatgc
      961 cgaagtacgc gaagttcggg gtcacgccgt cggcgagcgc gtagtagccc tcggcgtaga
     1021 cgccgtcccg gttgtcgccc tcgcgcgcca tcatcatcag cgagttgaag tcgccccagc
     1081 cctcgctcat gccggcgcac tgctgcgagc ccgcgccgcc gcagttttca agccggtggt
     1141 gcaggtagtg gccccattcg tgcgcgatca cggtgttgtc gagatcgccg tcgcgctccg
     1201 ggccctggac ggcgctggtg atctcgacgg tcaccgggcc cgccgcgagc gcggcctcga
     1261 gcgcggcgcc gtcggtcttg cccatcgcca cggacgggat gttggcggcg gcgctgccgg
     1321 cgaacgcgcg cggatcatcc agcgccgggt cgaccagcac gataccgatc gcgccggcgg
     1381 ccttgacgtt gttgacgacg ttcagcgagc cgcatccggt gatgccggag aagttgacca
     1441 ctgcgatctt gccggcgaca ttgctggtga ccgtctggca ggcgtcgtcg ttgggggcgg
     1501 tgccgtcggt ggcaaacgcc agatcgccga cgaggtcgaa gttgcggggg cccgcggcca
     1561 tcggctcggt gcgcggcgtg ccggtcgggc cggacaggcc ggtgagcgtg cccgcggtcc
     1621 acaggaacat gttcatggtc ggccgcgcgc cgtcggccgg cgtcgacatg ttggcgttgt
     1681 tgcgcgagcc gacgttcgcg ccggcttgcg cgtcgatccg cagcgggtca ttcgcgacgc
     1741 cgccgcgccc gaagttgtcg agctgggcgt tggcggtcgc ctcggtgaag cccgagtcgt
     1801 agtaccagtc gtgcatccag ttgaccagga agaacgtgtt gaccgccgcg gccgacgact
     1861 gcgtcgtgtt ggcgagcggc tccagcgtgt ggtcgtagac gtagttgagg acgcggcccg
     1921 acttgacgac cgggcggaaa tcgccggggg tgaacgagcg gttgccgtcg agatcggcga
     1981 acgcctcggc gttgttgccc gaggtggtcg tcgcgttggt cggcagccac gggtcgaagt
     2041 tcttgttgaa cgagtccatc acgaccaggt tggcggggag caagctgggc gccgagctgt
     2101 cgggcacgcc ggtcgggtgc ggcgcgaagc tcgccagcgc gccgtcgagc ggccggcggt
     2161 tgccggtggt ctccatgtac acgcggtaga cgaacgcgtc gttcgcgatg agattctcgt
     2221 cgtcgaggat cttcccgtcg gcgtcgcgga ccaggtagct gtgcgccgag aacgtcggga
     2281 tgctcgggtc ggccagggga tcgggggccg cgttgccctg gacctcgacc tcgtacgccg
     2341 ggaccagctg atcgccgacc tgcgcgaggg tgcgccgggc gcgcgcctcg tcgacctgga
     2401 gcgcggacgt cgaggcgacc tgcagcggct gccagccgcc ggtgtcaccg gaggccgggg
     2461 tctccgtgat cgacatctgc ggccgggccg ggccgaagtg ctggtcgagc gcgtgcgtca
     2521 gcgcatcggt cgccgacgac gcgaagctcg gcgcggccac ggcgggcagc agcgtgcccg
     2581 agatcgccgc gagcccgcca tcggcgccca gcaggacgtg gatctcgccg ccgtgcaccg
     2641 gcacgccatc gacgttctgg accagcttga tcatcgtggc cccgttgcga agctgctgca
     2701 cgccgttgtc ggccagcgcc atcggctgcg tgttgcgcac ccacagcgcc gacagcgtcg
     2761 cgacgtgatc gagcgcggcg gccgccgccg tcagcccggc cgcgctgggc cgcggcacga
     2821 tcgtccgcag cagacgcggc gctccggatg gctcgctcgc caggatcgcc acgccgaacg
     2881 aggtggcgag ctggtcggtc gagacagcgg acggcggcgc gccggggttc ggattccccg
     2941 ggtcgcccgg attcccgctg cccggcggcg cggacgtcga tgggccggtg gcacacgcaa
     3001 cggtgaatat caacggtgag acgagtgctc gagcacgaag catgatggga cctccctcgc
     3061 gacacgcagg ctacgtccat ccgcgatccc gttgcgcccc cggcggccga ctcggtgcta
     3121 acaccacatg cgcagtgtgc taacggatgg cgccgaggcg cctcgcagag ttcgcacgcc
     3181 ctgcgatccc tcccggcgtg tggcgcaagc gcccgggccg aacgcctacc gactgaaagg
     3241 tacgccgacg gaaaaccgag gcggactccc gcagcgcggc ccagtcggat cgagcggatg
     3301 gaatgcggct ccgtttctgg agcgctggac tttgtcatca cgcatacgcg agctggagac
     3361 cactggcgag cgcgaccggc gttgcccgtg caccgtaccg cgcgccggcc tggcggaaca
     3421 gatcgttcgc cagatcgaga tccagcgtga gctcgacctc gggagtcagc tcgcctcgct
     3481 cggcggctgc gcggcaccgg cggcgcgccg gccgctgacg atcacctcct cgcggccgtc
     3541 gtcggtcacg tggcgcacca gccggtaggt ggtgttgccc tcggcgcgcg ccacctcggg
     3601 ggcggcgatc aggagctgga ggtcgagggt cttgcacaga tcgaacagca aaccgaggtt
     3661 gtcgcgcgac agccgattgg cctcgtcgag gaacaggaac cgcaggctgc cggtcgtgcg
     3721 ccgggcgcgc agcaggttgg cgtcgcgctc ccactcggtg agcacgacca tcatgaccgc
     3781 ggcgccgacg ccgatcgcct cgccggtcga cagccgggtc gggctggcgg tctcccagtc
     3841 ggcggcggcc tggcggcgga tctggacggc gagctcgagg tactcgcggt agtcgagcag
     3901 gcgctcaccg ccggcgcggc cgccgccgta gcgccggaag atctcatcga gcgcctcctc
     3961 gatcgggatc gccgacagga acagcagctc ctgggcctcg ccgaggcgca gcgcgcgcag
     4021 gatctgctcc atgcggtcga tccggcgcag ctcgatccgg atgccgcgca cgctgccgaa
     4081 ctggatgccg tcgaggtgct ggttgagccg gcggatctgg ccggcggcgc ggcggagctg
     4141 gacgtcgatg ccgcgggcga cgtcctcgga ggcgccgcgc aggtcgtgct cctggcgagc
     4201 gagccgggcc tcgagcacgt cgaggtggct gcgcaggcgc tcgagcgccg cgagcggctc
     4261 gtcgacctcc acgacctggg ccggcacgcg gtggcgcagc cact
//
LOCUS       DASVOR010000340         8957 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_324549,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000340 DASVOR010000000
VERSION     DASVOR010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8957
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324549"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VF516_06965"
     CDS             <1..363
                     /locus_tag="VF516_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107453.1"
                     /translation="PQGAPEGAADPAPAADPRTPIERRRDAACDALGPRLTACAVADA
                     RADLAAGKIDQRQFERDTAPAVQRKNTEEFEARCKAARTSSRQVRVLEVCFRAEAQCA
                     PLLDCLAHLHDRAPAGAP"
     gene            complement(378..1616)
                     /locus_tag="VF516_06970"
     CDS             complement(378..1616)
                     /locus_tag="VF516_06970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107454.1"
                     /translation="MDVRTVGGRYAIDGRLGEGGMGQVYRARHLQLGKAFALKIISPT
                     FALDGAARARFNEEAKLASEISHPNIVSVVDFGEDAQFGAYMVMELIEGEPLTHEQAA
                     PMAVRRACDVLGQIADALDHIHRRGIVHGDVKADNIMVTAETTGARQRRLARLLDFGL
                     ARRSGGDGDDGLNGSPHYLAPERAAGGPPSVAADIYALGVLGYLMLTGTLPFDGSVIE
                     ILMAHINREVTPPSARRGESIEPVVEQLILRALAKDPAKRHGSAAAFRAELNAAMDAL
                     DQGRRPPSGPTVSVDRRDAILIAAFERSRLPQALVSLDGDIVLANKGFARLLGVERGL
                     EGMNISETTLATVVPGFSRAVRAAHHSGQTCERRARVFRGPGAPALELTLWFSPLGAP
                     GLEVHLLVRVEEADPRRRDE"
     gene            1765..1839
                     /locus_tag="VF516_06975"
     tRNA            1765..1839
                     /locus_tag="VF516_06975"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1800..1802,aa:Arg,seq:cct)
     gene            2354..2590
                     /locus_tag="VF516_06980"
     CDS             2354..2590
                     /locus_tag="VF516_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107455.1"
                     /translation="MKKLVFEPFMGIGPRRFFDLFSMKLSSGYPLKRKQKPDRAKWKV
                     GDEALVRLPMPRTTYLEREARVVQLVTTLLQETP"
     gene            2587..2763
                     /locus_tag="VF516_06985"
     CDS             2587..2763
                     /locus_tag="VF516_06985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107456.1"
                     /translation="MKLMIRIDDDETRALWETAKRAKAEVESWPAWKHNEVPRASSDA
                     AASDASTETSRDEG"
     gene            complement(2887..3099)
                     /locus_tag="VF516_06990"
     CDS             complement(2887..3099)
                     /locus_tag="VF516_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107457.1"
                     /translation="MSRVALDTGELTPVAQLPKMPLHPRFDTRLMFAADGVLLAPDVE
                     LGMDIWEAVPDEERKAVPPVEPSLNL"
     gene            complement(3466..3678)
                     /locus_tag="VF516_06995"
     CDS             complement(3466..3678)
                     /locus_tag="VF516_06995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107458.1"
                     /translation="MDDVAVGQSRLSNLRADLARCQASDRARLAFGAARDALGGALLL
                     ILLALSLVRRGFLIGWHELALRFRAP"
     gene            3763..4026
                     /locus_tag="VF516_07000"
     CDS             3763..4026
                     /locus_tag="VF516_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107459.1"
                     /translation="MGNLSYHMDANSIASLFAQHGEVADVHIVLDQATRRPRGFAFVT
                     MGSPAEAQAAIVELDGRNVDGRTLRVNQAEARESGPSMGRGRY"
     gene            complement(4210..5304)
                     /locus_tag="VF516_07005"
     CDS             complement(4210..5304)
                     /locus_tag="VF516_07005"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8107460.1"
                     /translation="MSAIAGPARFDESDDVWLGHRSWDEYVHDAAEFRGHGIVVLTAP
                     HPAASPGARYGINMISRGWNVGWVLDGDPEHGYWLAIDENGNGVLGDDPVRKLAHGPA
                     DWRVEVTTPPRVAGGPPLRTIVIFDGKMVRSYASTLRTGELVIDGRPLRFTISCELAD
                     CSDPRAVHLGFDLDGDGAVDLGPGSYEHYRPGDRTVVAFGRSYDFDIAPDGSRLTLRR
                     SQVAHAPRPTLRPGSPAPAFEARDDPARFSLAAARGHVVLLDFFSEGCHFCIEDLPWV
                     ASVHDRYAGAGLELVTIAAATPPPGAHPWPVIVEHDAAPVAALFRVEAYPTYFVIDRD
                     GTIACARCRREQVDRALAQLLADRSTTGAP"
     gene            complement(5587..8262)
                     /locus_tag="VF516_07010"
     CDS             complement(5587..8262)
                     /locus_tag="VF516_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107461.1"
                     /translation="MLFESGQVRPLALSPSGKLLFAVNTPDNRLEVFRVGSSGLVHRA
                     SVPVGLEPVAVAAPSDDEAWVVNHLSDSVSVVRFSDDGRGGAVVRTLLVGDEPRDVVF
                     AGPGKTRAFITTAHRGQNAPFDPQLTTPGVGRADVWVFDARHLGNSLGGDPLTIVTLF
                     SDTPRALAVTPDGSRVFAAAFHSGNRTTGINESVVPDGGQAAGGVPGPSTNAAGVPAP
                     EVGLIVKHDGQHWVDSLGRPWDDQVKFSLPDKDVFVIDATANPPAQVAGPAGWFAGVG
                     TILFNMAVNPVNGHVYVTNTEARNDLRFEGPGTFVGTSLRGHLHESRITVLSSAGAAP
                     RHLNKHIDYSAANQNGVGDKSLAQPLGMAVTSDGAKLYAAAFGSSRIGIYDTAALESD
                     SFVPGTDNQIAVTGGGPTGLVLDEARGRMYVLTRFDNAISVIRTATRQEIAHVPMYNP
                     EPPSVTAGRRFLYDARLSSSNGEAACGSCHVFGDFDSLAWDLGNPDAAVLTNPGPFVP
                     PLPGFGTDPLFGLPASFHPMKGPMVTQSLRGMANHGPMHWRGDRTGGNDAPSAQPGSG
                     AFDEQAAFKKFNPAFVNLLGRTAELSPDQMQVFTDFILQITYPPNPIRNLDNSLTPAQ
                     QAGRDFFFGVTVSLVGGTCESCHRIDPDANPSEGPFAGLFGTDGRSSFDGEPQMVKVP
                     HLRNMYQKVGMFGTSAVVGSLGPDPFLGDQVRGFGFSHDGTIATMFRFNSGFDLSPFN
                     PVGLPITPESAQAKRNMEQFMLAFDTNLAPIVGQQVTLTAATRQAASPRIDLLMVRAD
                     AGECDLVAKGRIQDEVGFLYLGGGRFQADRQALPPILDRDLRRLVTATGAALTYTCVP
                     PGSGRRIGIDRDLDGALDGDERAAGSDPADPHSTP"
BASE COUNT         1356 a         3092 c         3207 g         1302 t
ORIGIN      
        1 ccccagggcg ctcctgaggg cgcggccgac ccggcccccg ccgccgatcc gcgcaccccg
       61 atcgagcgcc gccgcgacgc tgcctgcgat gccctcggcc ccaggctcac cgcgtgcgcc
      121 gtggccgatg cccgcgccga cctcgccgcc ggcaagatcg accagcgcca gttcgagcgc
      181 gacaccgctc ccgccgtcca gcgcaagaac accgaggagt tcgaggcccg gtgcaaggcc
      241 gcgcgcacct cgtcgcgcca ggtccgcgtg ctcgaggtgt gcttccgcgc ggaggcccag
      301 tgcgcgccgc tgctcgactg cctcgcccat ctccacgatc gcgcccccgc cggcgccccg
      361 tagcccgtcg ctcgtcgtca ctcgtcccgg cggcgcgggt cggcttcctc gacccgcacc
      421 agcaggtgga cctcgagccc gggcgcgccg agcggcgaga accacagcgt cagctcgagc
      481 gccggcgcgc cggggccgcg gaacacccgg gcccgccgct cgcatgtctg gccgctgtgg
      541 tgcgcggcgc gcaccgcgcg ggagaacccc ggcaccaccg tcgccagggt cgtctcgctg
      601 atgttcatcc cctcgagccc gcgctcgact ccgagcagcc gcgcgaaccc cttgttcgcc
      661 agcacgatgt caccatcgag cgacaccagc gcctgcggca gccgcgaccg ctcgaacgcc
      721 gcgatcagga tcgcgtcgcg ccggtcgacc gacaccgtcg gcccgctcgg cggccgccgg
      781 ccctgatcca gcgcgtccat cgccgcgttg agctcggcgc ggaacgccgc cgccgacccg
      841 tgccgcttcg ccgggtcctt ggcgagcgcc cgcaggatca gctgctcgac caccggctcg
      901 atcgactcgc cgcggcgcgc cgacggcggc gtcacctcgc ggttgatgtg cgccatcagg
      961 atctcgatca cgctgccatc gaacggcagc gtcccggtca gcatcaggta gccgagcacc
     1021 ccgagcgcat agatatcggc cgcgacgctc ggcggcccgc ccgccgcccg ctccggcgcc
     1081 aggtagtgcg ggctgccgtt cagcccgtcg tcgccgtcgc cacccgaccg ccgggccagc
     1141 ccgaagtcga gcagccgcgc cagccgccgc tgccgcgctc cggtcgtctc ggccgtcacc
     1201 atgatgttgt cggccttcac gtccccgtgc acgatgccgc ggcggtggat gtgatccagc
     1261 gcatcggcga tctgccccag cacatcgcac gcccgccgca ccgccatcgg cgccgcctgc
     1321 tcgtgggtca gcggctcgcc ctcgatcagc tccatcacca tgtacgcccc gaactgcgcg
     1381 tcctcgccga agtcgaccac cgagacgatg ttggggtgcg agatctcgct cgcgagcttg
     1441 gcctcctcgt tgaaccgggc tcgcgccgcc ccgtcgagcg cgaacgtcgg cgagatgatc
     1501 ttcagcgcga acgccttgcc gagctgcagg tggcgggcgc gatagacctg gcccatccct
     1561 ccctcgccca gccgaccatc gatcgcgtac cgcccgccca cggtacgtac atccatgggc
     1621 ctgactgtag atcagtcggg gcaccgtacc gggttgaagt tgtccatttc cgtgcgaatt
     1681 cgcggctaac tcagacgaac cattgggctc gggggtcgga gacttgcaca ccgccgcccc
     1741 ctcgggcaga tttccggcgg tccaggtccc atagctcagc tggatcagag cggcgctttc
     1801 ctaaagcgta ggtcgcaggt tcgagtcctg ctgggatcac cggaatcatt caggttggtg
     1861 gcctggcgcg gctaggacac ggccgctagg gcacaactag gacacgagaa ctgcgatcac
     1921 ctgtagcgct gcccatgcga gcggatcgag gcgtccggcg gcacgccgcg ggcatcccgt
     1981 cgacggccga catgcgcgag gcgtcccacg cctcgacatc cgcgcgtcgg tactcgcgtg
     2041 aggccacaga cgtcgcagca catgagcacg gccccgcgga gcgtgcgggg gctgaactgg
     2101 gcgccagtcg tctgctcggt ggtgaagtgg cggcgggaac gcttggaact cgacatcggt
     2161 ctcctggcag ccgggtgcgt accacggcgg tctgaccgtc acgttccagc cgaggcagaa
     2221 catcgggtgc tcggtcctgc gcgcacgaac cgacgagcga caccgtcttg cagtcaggcc
     2281 cgcagtacac ttggcccgtg gtcgcagcac gggcagatgt ggaacaagcc caggcaccat
     2341 cgacggcgct gccatgaaga agctcgtgtt cgagcccttc atgggcatcg gtccgcgaag
     2401 gttcttcgac ctgttctcga tgaagctgag cagcggatac ccgttgaagc gaaagcagaa
     2461 acccgacagg gccaaatgga aggtcggcga cgaggcgctc gtgcgccttc cgatgccacg
     2521 aaccacgtac cttgaacgcg aagcccgcgt tgtgcagctc gtgaccacgc tcttacagga
     2581 gacaccatga agctcatgat ccggatcgac gacgacgaga cccgggcact gtgggagacc
     2641 gccaagaggg ccaaagccga ggtcgagagc tggccggcct ggaagcacaa cgaggtgcca
     2701 cgcgcatcct ccgatgccgc tgcctcggat gcgagcacag agacgtcgag agacgagggc
     2761 taagctaccg ggcgcgggtt acctcagaag gaatgccgtc gtcgtgccct gtccgcgctc
     2821 cgagttcgca gcgacgctga cccggcagat cagctcggct cactggccgc acgcgatgcg
     2881 tccgcgctac aggttgaggc tgggctcgac cggcggcact gccttgcgct cctcgtcggg
     2941 gacggcctcc cagatgtcca tgccgagctc cacgtcgggc gcgaggagga cgccatcggc
     3001 cgcgaacatg aggcgggtgt cgaaccgcgg gtgaagtggc atcttcggca actgcgcgac
     3061 cggggtcagc tcgccggtgt cgagtgcgac gcgcgacacc accggcagct ttcgcggaag
     3121 cgcccggacg cgacagccgt gataggtcca caggtcggcg ccagctcggt gccggcccgt
     3181 cgaaggcctc catctcctcg gccttgtcca gtgcaggacc tgcccatccg tgctacggat
     3241 ccagtccttc acagcgcgtt cgactgcccg cgacgttccg cacgggtgat gcgtagctgg
     3301 ggggaacccg aacggcaccg attgtctcgc tggaaccacg ctgcgcaccc gctcgatcct
     3361 gcaaccgagc tccttggacc gctcgatcgg ggtggacacg gtttccgagc ctgcgcggtc
     3421 cgccgagcgt gccgccgacg gcatggtcca cgagctgcgc cggcgtcacg gagccctgaa
     3481 ccggagagct aattcgtgcc aaccaatcag aaaaccacgt cgaacaagcg acagcgcgag
     3541 caggatcaga aggagcgcgc cgccgagcgc atcgcgcgcc gcaccgaacg caaggcgcgc
     3601 gcggtcgctc gcctggcatc gggccaggtc ggcccgcaaa ttgctgagcc tgctctgccc
     3661 gaccgcgacg tcgtccacga cgaccccgaa gcctcgacga caccgtccgc ggtggcaggc
     3721 cacaggcgcc ccaggcagtc accgacggcg tgcaggctct atgtgggcaa tctgtcctac
     3781 cacatggacg cgaattccat cgcgtcgctg ttcgcgcagc acggtgaggt cgccgacgtg
     3841 cacatcgtgc tcgatcaggc cactcggcgc ccgcgcggat tcgcgttcgt gaccatgggc
     3901 tcgccggcgg aggcgcaggc tgcgatcgtc gagctcgacg gccggaacgt cgacggccgc
     3961 acgctgcgcg tgaaccaggc ggaggcccgc gagtcgggtc cgagcatggg ccgcggacgt
     4021 tactgaccgg ccgaggtgac gctcacgggt gaccggcggt cggccgcgcg gcaacggcgg
     4081 cccacgccgc ggtggcgatc gtggctgggg ccgtggccgc taacgccgag ccagcgcgcg
     4141 acgcgctcgc gggatcgcgc atcgcggatt cgtgttcggt gcgcgagcag cccaccacgc
     4201 gctgagcggt cagggcgcgc cggttgtgga tcgatcggcg agcagctgcg cgagcgcgcg
     4261 atcgacctgc tcgcgccggc agcgcgcgca ggcgatcgtg ccgtcccgat cgatcacgaa
     4321 gtaggtcggg taggcctcga cgcggaacag cgccgccacc ggggcggcgt cgtgctcgac
     4381 gatgaccggc cagggatgcg cgccgggcgg cggggtggcg gcggcgatcg tgacgagctc
     4441 gagcccggca ccggcgtagc ggtcgtgcac gctggcgacc cacggcaggt cctcgatgca
     4501 gaagtggcag ccctcgctga agaagtcgag caggacgacg tggccgcggg cagccgcgag
     4561 cgagaaccgc gcgggatcgt cccgtgcctc gaaggccggc gcggggctgc cggggcgcag
     4621 cgtcggccgc ggcgcgtggg cgacctgcga gcgccgcagc gtcaggcggc taccgtcggg
     4681 ggcgatgtcg aagtcgtagc tccgcccgaa cgcgaccacg gtccgatcgc cgggccggta
     4741 gtgctcgtag gaaccggggc cgagatcgac ggcgccgtcg ccgtcgaggt cgaagccgag
     4801 gtgcaccgcc cgtgggtcgc tgcagtcggc gagctcgcag ctgatcgtga accgcagcgg
     4861 acgaccgtcg atcacgagct cgccggtgcg gagggtgctc gcgtaggagc gcaccatctt
     4921 gccgtcgaag atcacgatgg ttcgcaacgg cgggccaccg gcgacgcgcg gcggggtcgt
     4981 cacctcgacg cgccagtcgg ccgggccgtg cgcgagcttg cggaccgggt cgtcgccgag
     5041 cacgccgttg ccgttctcgt cgatcgcgag ccagtagccg tgctccggat cgccgtcgag
     5101 cacccagccg acgttccagc cgcggctgat catgttgatg ccgtatcgcg cgccgggcga
     5161 cgcggccggg tgaggcgcgg tgagcaccac gatcccatgg ccgcggaact cggcggcgtc
     5221 gtggacatac tcgtcccagg accggtggcc gagccacacg tcatccgact cgtcgaaccg
     5281 tgccgggccg gcgatggcgc tcacgaccga tggatccgga gcggcggacg gcgccggcgc
     5341 gtggcaagcg gccacgaccg cgccccatgc gcccagcagc gccaccgcgc cgcgttgcat
     5401 ccctcgcaca ccatgccagg aacgatccag catcctgcgg gccgccatgc gggtgatata
     5461 ccgctccggg ggcgcacgga cgatgcgtcc agatccgcgg cacgcctcga tcgtgggccg
     5521 cggcgccgcg ccggggcgtg ccagactggc ggcgtcctgg ccccgccgcg cgtcagcctg
     5581 acaaggctac ggcgtgctgt ggggatctgc cggatcgctg ccggcggcgc gctcgtcgcc
     5641 atcgagagcc ccgtcgaggt cgcgatcgat gccgatgcgc ctgccggatc ccggcgggac
     5701 acaggtgtag gtcagcgcgg cgccggtcgc ggtgacgagc cggcgcaggt cgcgatcgag
     5761 gatcgggggc agggcttgcc ggtctgcctg gaaccgacca ccgccgaggt agaggaaccc
     5821 gacctcgtcc tggatgcggc ccttggccac gagatcgcac tcgccagcgt ccgcgcggac
     5881 catgagcagg tcgatgcggg gcgaggccgc ctgccgggtc gccgcggtga gcgtcacctg
     5941 ctggccgacg atcggcgcga ggttggtgtc gaacgccagc atgaactgct ccatgttgcg
     6001 cttggcctgc gcgctctccg gcgtgatcgg cagaccgacc gggttgaacg ggctcaggtc
     6061 gaacccactg ttgaaccgga acatcgtcgc gatggtccca tcgtggctga agccgaagcc
     6121 gcggacctgg tcgccgagga acggatcggg gcccagcgag ccgaccacgg cgctcgtgcc
     6181 gaacatgccg accttctggt acatgttccg gaggtgcggg accttgacca tctgcggctc
     6241 gccgtcgaag gacgagcggc cgtcggtccc gaacaagccg gcgaacgggc cctcgctcgg
     6301 gttggcgtcc ggatcgatcc ggtggcacga ctcgcaggtc ccgccgacca ggctgaccgt
     6361 gacgccgaag aagaagtcgc gacccgcctg ctgggccggc gtgagcgagt tgtcgaggtt
     6421 gcggatcggg ttgggtggat acgtgatctg gaggatgaag tcggtgaaca cctgcatctg
     6481 gtcgggtgac agctccgcgg tccggccgag caggttcacg aacgccgggt tgaacttctt
     6541 gaacgcggcc tgctcgtcga acgccccgga gccgggctgc gcgctgggcg cgtcgttgcc
     6601 gccggtccgg tcgccgcgcc agtgcatcgg gccgtggttg gccatgccgc gcaggctctg
     6661 cgtgaccatc ggtcccttca tcgggtggaa gcttgcaggc aggccgaaca gcggatccgt
     6721 gccgaacccg gggagcggtg ggacgaacgg cccggggttg gtgaggaccg ccgcgtccgg
     6781 gttgccgaga tcccacgcca ggctgtcgaa gtcgccgaat acgtggcagc tgccgcaggc
     6841 cgcctctccg ttgctcgagc tcagccgggc atcgtagagg aaccgccgcc cggcggtgac
     6901 gctcggcggc tcggggttgt acatcgggac gtgggcgatc tcctggcgcg tggccgtgcg
     6961 gatgacggag atcgcgttgt cgaaccgggt gaggacgtac atgcgcccgc gggcctcgtc
     7021 gaggacgagc ccggtcgggc cgccgccggt caccgcgatc tggttgtccg tgccgggcac
     7081 gaacgagtcg ctctcgagcg cggcggtgtc gtagatcccg atccgcgacg agccgaacgc
     7141 ggcggcgtag agcttcgcgc cgtcgctggt cacggccatg ccgaggggct gcgcgaggga
     7201 cttgtcgccg acaccgttct ggttcgcggc gctgtagtcg atgtgcttgt tgaggtggcg
     7261 cggcgccgcg cccgcggagc tcagcacggt gatccggctc tcgtgcaggt ggccgcggag
     7321 gctggtcccg acaaacgtgc cggggccctc gaaccgcagg tcgttgcggg cctcggtgtt
     7381 ggtcacgtag acgtgtccgt tcaccggatt gacggccatg ttgaacagga tggtgccgac
     7441 gccggcgaac cagcctgccg ggccggcgac ctgggccggc gggttggccg tggcgtcgat
     7501 gacgaacacg tccttgtcgg gcagcgagaa cttgacctga tcgtcccagg ggcggccgag
     7561 gctgtcgacc cagtgctggc cgtcgtgctt gacgatcagc ccgacctcgg gggccgggac
     7621 cccggcggcg ttggtgctgg ggccggggac accgccggcc gcctgtccgc cgtcgggcac
     7681 cacggactcg ttgatccccg tggtgcggtt gccggagtgg aacgcggcgg cgaagacgcg
     7741 cgagccgtcg ggggtgaccg cgagcgcgcg gggggtatcg ctgaacagcg tgacgatggt
     7801 gagcggatcg ccgccgagcg agttgccgag gtggcgggcg tcgaacaccc agacgtcggc
     7861 gcggccgacc ccgggcgtgg tgagctgggg atcgaacggg gcgttctgcc cgcgatgggc
     7921 ggtggtgatg aaggcgcggg ttttccccgg ccctgcgaac acgacgtcgc ggggctcgtc
     7981 gccgaccagc agtgtgcgga cgacggcgcc accgcggccg tcgtcggaga acctgaccac
     8041 actgacgctg tcggagaggt ggttcacgac ccaggcctcg tcgtcgctcg gggccgcgac
     8101 cgcgaccggc tcgagcccga cgggcaccga ggcgcgatgg accaggccgc tcgatcccac
     8161 cctgaacacc tcgagacggt tgtccggggt gttgacggcg aacaggagct taccgctcgg
     8221 ggacagggcc agggggcgga cctgaccgct ctcgaacagc atgaacggcg atcgggtaga
     8281 acctgcggca cgggcgatcg gttcgatgcg tggccgtggt tcggcgggcg cacgggcgat
     8341 ctgatcgggt ggtggctcca gatcgccggg ttgcgtgcag gcgatcacgt cgaccagcag
     8401 cgcagtgaat aatgccttcg atatcgagga tcgagtcagc gcgggaatca gtgcggcttt
     8461 catggccatc tccatttccg tcctgcggtg cagccgatcc accttcgcat aggacgagca
     8521 tcgacgctcg cccacctcgg cggtcgctgt gctcgaggag gatgaccgct cccgagacga
     8581 tgcgagcgat ttttggggta cgtcaacggt gcagcgcgat gcgacgccac acgatgcaac
     8641 agctccgtat cggtgatagc gccgtcgatg tcatacagca catcagatat ctgtggtcgg
     8701 gggaggcgct gtgaactcgg gacggaattt tcgagtgcga acatgtcgcc gagatcggta
     8761 tacgaggctg tgccgtcatg tgccagaagt ggatctggac tcatgcggcg cgccggggcg
     8821 gtcggcgggg atcagaccgc agcgctcccc gacggatcgc ggccgttcac agtccggcct
     8881 tggcgcgctt cgccttgatg cgctcgaagc tcttggcctt gtcggcgatg aagcgcacgt
     8941 gcctgccacg gccgcat
//
LOCUS       DASVOR010000341         6383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_470793,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000341 DASVOR010000000
VERSION     DASVOR010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470793"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(42..497)
                     /gene="fabZ"
                     /locus_tag="VF516_07015"
     CDS             complement(42..497)
                     /gene="fabZ"
                     /locus_tag="VF516_07015"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019171 -
                     (3R)-hydroxyacyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEX8107462.1"
                     /translation="MAPDVAMKVEQILGLLPHRYPFLLIDRVLEVSADRVRALKNVTF
                     NEPYVQGHFPGAPVMPGVLQIEAMAQAGGVLAVQTVAFDPATHVMLFMAIDAVKFRKA
                     VVPGDQLIIEVVPLRKGKIFKMKGEIQVDGQVVSSAEFLAGLVEKSKVA"
     gene            complement(527..1015)
                     /locus_tag="VF516_07020"
     CDS             complement(527..1015)
                     /locus_tag="VF516_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEX8107463.1"
                     /translation="MVSPLLGGIGSFARGAVPANPKIGIIDIENTLSTTPAGKRANEA
                     FEKTRKAKQATLDKEQEELKKADADLVKQKAVLKPEVFDAKRQELEKKFVDLQQTYVK
                     LERELAQDRTKLIQDLLKQAEPKIAALAKAEGVSLILDQSATVWADPSVDLTQKLNAE
                     MK"
     gene            complement(1200..3662)
                     /gene="bamA"
                     /locus_tag="VF516_07025"
     CDS             complement(1200..3662)
                     /gene="bamA"
                     /locus_tag="VF516_07025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEX8107464.1"
                     /translation="MKRSLLVFLACAATIGLSGGPVRRAFAQPSSQAAAGGPVTPGPA
                     VQGRPIEKVQFRGNRKVEDDAIRVQLLSKPGTLFDAAKLREDIRAMWKMGFFADVNVE
                     AEIGPAGGVTLTFAVKEKPSIRKVLIAGNHELGLDKINEVIDLELDTIVDIAKIKKNR
                     EKIADQYVQKGFYLATVDYELKPVNEAEVDVWFKIDEKAKVKIRDVQFIGNRSIPDDE
                     LRLAIQTRREDALSFLNDSGVYSQEAFERDLLLVSAHYWDKGYANVKVGTPQLRLSRD
                     KEYMYLSIPIDEGPVFTIGQVNFKGDLVGTPADNLAKIKMRPGTTFARTLIAEDREKL
                     SAFYQDLGYAYANVSPLTKVDLPNRKISLTYEVARGKRAYFERINIRGNSKTRDKVIR
                     REMKISEGDLFNNTNLEVSKRRITALGYFENVTVSTKRGSSDEFVEVNVEVSERPTGT
                     FQIGAGFSSVENFIAQAQISQNNLFGRGQTLSVQAQLSSLRQLFLLRFVEPYFLDTLW
                     TFGFDLYNQSIGFGTFFRNSSGGQLTWGYPLSYEARAFLVYKLEDVGITTGTGGITSL
                     GASQVPIAAASVANLFRGGWTSSLRASMQWDSRNNRLFPTNGWYATTFLELANKYTGS
                     ENKFVRWGGFLRHYRELWGPFVFHGNAEIGVTTSTDPLGVPISERYLVGGIFDIRGYA
                     PRSLGPQLLTQTPGDVGQTLGRLPLGGNLQLIFNGEVEFPLFKKIGISGVVFFDAGNA
                     YNLEDRYCSGLQRKNAAVSIKFDPCFRPLDSLTSGLRRSVGFGFRWFSPIGPLRFEWG
                     IPLDAQPGEDPLVFEFTIGNFL"
     gene            complement(3659..4564)
                     /locus_tag="VF516_07030"
     CDS             complement(3659..4564)
                     /locus_tag="VF516_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8107465.1"
                     /translation="MTDAPEPALPTPPHPAADPAIDAPSAAANPPAATAPDASPVPAR
                     GRGAHIVVEKLGKAYFHGGKALPILWDVNLTLQPGDMVAVVGASGVGKSTFLQILGTL
                     DLPTSGSIQFDGEELTTMSPSRLAEFRNRRIGFVFQFHHLLPDFTALENVMMPALIQR
                     LATERARRKARDILGRVGLSHRLTHRPGELSGGEQQRVALARAMVLEPSLLLADEPTG
                     NLDRSTGEAIHQLFLDLNRERGSTLLVVTHNPDLAVLMPRRLRMVDGGRLIEEHGDLP
                     YRAPGAPASEPAPDPIPPPDAGAVP"
     gene            complement(4698..5234)
                     /locus_tag="VF516_07035"
     CDS             complement(4698..5234)
                     /locus_tag="VF516_07035"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8107466.1"
                     /translation="MKTALVLTSACFAISSTASADTYLGVALGSQPGINDAFVQAVGQ
                     PSGRSLRGLVGMRFGRLSLEGALNGFSVFTSRFGEQTAYQASGALKLNLPLGNNFEAF
                     ARGGIERTWLNQSSDGFDLSGTGYLVGGGFEYRLDAMIANASIFVDYNIHHAGLDAAR
                     FSVDATEQIWGLGILVGL"
     gene            complement(5333..>6383)
                     /locus_tag="VF516_07040"
     CDS             complement(5333..>6383)
                     /locus_tag="VF516_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8107467.1"
                     /translation="TPAARPGRRWLRRAGRERRFRCRVGQRLQRPGGVPGAVIDPPPP
                     DLVTSDGPPTDYSQPDYLQDAVKVIDPPFDTPSISRRTQSLPGILVGRELVKQIHLYT
                     GEEIRVVSPLSDPANPDANGTPIPFNRDYRVAGTFFTGMYEYDLKYVYVTLDSLQEFL
                     DRGDAIDGIEVRVVNPYDSDAFVAEFQAVFGPAYRVQDWKELNRSLFSALKLEKIAMF
                     LVLGIVILVASFSILGNLIMVVVEKAREIALLKTLGASNAGVMQLFAIQGLMIGLIGT
                     ALGVVTGLGGCWALSEYGFPLNENVYYIDRLPVHVDPWSVAAVAAAGVVISIGATVYP
                     AIVAARVRPAAGMRH"
BASE COUNT         1032 a         2108 c         2202 g         1041 t
ORIGIN      
        1 cgcgagcgcg agcgcgtgcc gggccatggc gcgcagatcc gtcacgcgac cttcgacttc
       61 tcgacgagcc cggcgaggaa ctcggccgac gacacgacct ggccgtcgac ctggatctcg
      121 cccttcatct tgaagatctt gcccttgcgc aacggcacga cctcgatgat cagctggtcg
      181 ccggggacca ccgccttgcg gaacttcacc gcgtcgatcg ccatgaacag catgacgtgg
      241 gtcgccggat cgaacgccac ggtctgcacc gcgaggacgc cgccggcctg ggccatcgcc
      301 tcgatctgca gcacgccagg catcaccggc gcgcccggga agtggccctg gacgtacggc
      361 tcgttgaacg tgacgttctt gagcgcccgc acgcgatcgg cgctgacctc gaggacgcga
      421 tcgatgagca ggaacgggta gcgatggggc agaaggccga ggatctgctc gaccttcatc
      481 gcgacgtcag gcgccatatg cgccaggttg tatcacccga tggcggtcac ttcatctcgg
      541 cgttgagctt ctgggtgagg tcgaccgacg ggtcggccca caccgtggcg ctctgatcga
      601 ggatcagcga aacgccctcg gccttggcga gcgcggcgat cttcggctcg gcctgcttca
      661 gcagatcctg gatcagcttg gtgcgatcct gggcgagctc gcgctcgagc ttgacgtagg
      721 tctgctgcag atcgacgaac ttcttctcga gctcctggcg cttggcgtcg aacacctcgg
      781 gcttgagcac cgccttctgc ttgaccagat cggcgtcggc cttcttgagc tcctcctgct
      841 ccttgtcgag cgtcgcctgc ttggccttgc gcgtcttctc gaacgcctcg ttggcgcgct
      901 tgccggccgg cgtggtcgac agcgtgttct cgatatcgat gatcccgatc ttggggttcg
      961 ccggcacggc gcctcttgcg aacgacccga tcccgccgag cagcgggctc accatcagac
     1021 caaggccaag acaacccaga gcgacgtagc gattcatggc agacctcgac gcttcatacg
     1081 acgattggac cggggttcca agacggaatt cacacccatc gtagcatcgc cgcatcgcgc
     1141 cagatcgcgg cgagccggag gccgaccgcg gggctgaccg gggctgaccg ggagctgaat
     1201 cagaggaagt tcccgatcgt gaactcgaac accagcgggt cctcaccggg ctgcgcatcc
     1261 agcgggatgc cccattcgaa ccgcagcggg ccgatcggcg agaaccaccg gaacccgaag
     1321 ccgaccgacc tgcgcaggcc ggaggtcagc gagtccagcg gacggaagca cgggtcgaac
     1381 ttgatcgaga ccgccgcgtt cttgcgctgc aggccggagc agtagcggtc ctcgaggttg
     1441 taggcgttgc cggcgtcgaa gaacacgacg cccgagatcc cgatcttctt gaacagcggg
     1501 aactcgacct cgccgttgaa gatcagctgc aggttgccgc cgagcggcag ccggccgagg
     1561 gtctgcccga cgtcgcccgg ggtctgggtc agcagctgcg ggccgagcga gcgcggcgcg
     1621 tagccgcgga tgtcgaagat gccgccgacc aggtagcgct cgctgatcgg cacgccgagc
     1681 ggatcggtcg acgtggtgac gccgatctcg gcgttgccgt ggaacacgaa cgggccccac
     1741 agctcgcggt agtggcgcag gaagccgccc cagcgcacga acttgttctc cgagcccgtg
     1801 tacttgttgg cgagctcgag gaacgtggtc gcgtaccagc cgttggtcgg gaacagccgg
     1861 ttgttgcgcg agtcccactg catcgaggcg cgcagcgacg acgtccagcc gccgcggaac
     1921 aggttggcca cgctcgccgc ggcgatcggt acctggctcg cgccgaggct ggtgatcccg
     1981 cccgtgccgg tggtgatgcc gacgtcctcg agcttgtaga ccaggaacgc gcgggcctcg
     2041 tacgacagcg ggtagcccca ggtcagctgg ccgcccgacg agttgcggaa gaacgtgccg
     2101 aacccgatgc tctggttgta gaggtcgaag ccgaacgtcc acagcgtgtc gaggaagtac
     2161 ggctcgacga accgcagcag gaacagctgg cgcaggctcg acagctgggc ctggaccgac
     2221 agggtctggc cgcggccgaa caggttgttc tgcgagatct gggcctgggc gatgaagttc
     2281 tcgaccgacg agaacccggc gccgatctgg aacgtgccgg tcggccgctc ggagacctcg
     2341 acgttgacct cgacgaactc gtccgagctg ccgcgcttgg tcgacaccgt gacgttctcg
     2401 aagtagccga gcgcggtgat ccggcgcttg gacacctcga ggttggtgtt gttgaacaga
     2461 tcgccctcgg agatcttcat ctcgcggcgg atcaccttgt cgcgggtctt ggagttgccg
     2521 cggatgttga tccgctcgaa gtacgcgcgc ttgccgcggg cgacctcgta ggtcaggctg
     2581 atcttgcggt tcggcagatc gaccttggtc agcggcgaga cgttggcgta ggcgtagccc
     2641 aggtcctggt agaacgccga cagcttctcg cggtcctcgg cgatcagcgt gcgcgcgaac
     2701 gtggtgcccg gccgcatctt gatcttggcg aggttgtcgg ccggtgtccc gaccaggtcg
     2761 cccttgaagt tgacctggcc gatcgtgaac accgggccct cgtcgatcgg gatcgacagg
     2821 tacatgtact ccttgtcgcg cgacagccgg agctggggcg tgccgacctt gacgttggcg
     2881 tagcccttgt cccagtagtg cgccgacacc agcaggagat cgcgctcgaa cgcctcctgg
     2941 ctgtacacgc cggagtcgtt gaggaacgac agcgcgtcct cgcgccgggt ctggatcgcg
     3001 agccggagct cgtcgtcggg gatcgaccgg ttgccgatga actggacgtc gcggatcttg
     3061 accttggcct tctcgtcgat cttgaaccac acgtcgacct cggcctcgtt gaccggcttg
     3121 agctcgtagt cgacggtggc gaggtagaac cccttctgga cgtactgatc ggcgatcttc
     3181 tcgcggttct tcttgatctt ggcgatgtcc acgatggtgt cgagctcgag gtcgatcacc
     3241 tcgttgatct tgtcgagccc gagctcgtgg ttgccggcga tcaggacctt gcggatcgac
     3301 ggcttctcct tgaccgcgaa ggtcagcgtc accccgccgg cgggcccgat ctcggcctcg
     3361 acgttgacgt cggcgaagaa ccccatcttc cacatcgcgc ggatgtcctc gcgcagcttc
     3421 gcggcgtcga acagcgtgcc cggcttggac agcagctgga cgcggatcgc gtcgtcctcg
     3481 accttgcggt tgccgcggaa ctgcaccttc tcgatcggcc ggccctgcac ggcaggtccg
     3541 ggtgtcaccg ggccgccggc ggcagcctgc gagctcggtt gtgcgaacgc gcggcggacc
     3601 gggccaccgg agaggccgat cgtggccgcg catgccagaa acaccaacag ggaccgcttc
     3661 acgggaccgc tcctgcgtcg ggtggcggga tgggatcggg ggccggctcg gacgccgggg
     3721 cacccggggc acgatacgga aggtcgccgt gctcttcgat caggcgcccc ccgtcgacca
     3781 tgcgtagcct gcgcggcatg agcaccgcca gatcggggtt gtgggtgacg accagcagcg
     3841 tcgagccgcg ctcgcggttg aggtcgagga acagctggtg gatcgcctcg ccggtcgagc
     3901 ggtcgaggtt gccggtcggc tcgtcggcca gcagcagcga cggctcgagc accatcgcgc
     3961 gggccagcgc gacgcgctgc tgctcgccgc cggacagctc gcccggccgg tgggtgaggc
     4021 gatgcgacag cccgacgcgc ccgaggatgt cgcgcgcctt gcgccgcgcg cgctcggtgg
     4081 ccagccgctg gatcagcgcc ggcatcatca cgttctcgag cgcggtgaag tccggcagca
     4141 ggtgatggaa ctggaacacg aagccgatcc ggcggttgcg aaactcggcg agccgggacg
     4201 gcgacatcgt cgtcagctcc tcgccgtcga actggatcga gcccgaggtc ggcaggtcga
     4261 gcgtgccgag gatctgcagg aacgtcgact tgccgacgcc cgacgcgccg accacggcga
     4321 ccatgtcgcc gggctggagc gtgaggttga cgtcccacag gatcggcagg gccttgccgc
     4381 cgtggaaata cgccttgccg agcttctcga ccacgatgtg ggcgccgcgc ccgcgcgccg
     4441 gaaccgggct cgcgtcggga gcggtcgcgg ccggcgggtt cgcagccgcg ctcggggcat
     4501 cgatcgcagg atcggcggcc ggatggggag gcgtcggcag cgctggctcg ggggcgtccg
     4561 tcacgaggga atctctaaag agcgcaggac cttactcatg tggcgctgcg ggtgtcaacg
     4621 cgcgatggga ccaatgccga gccgcaatgg ttgcctgcac ctgcgcgcca cggccgcgcc
     4681 gccgtctacc cccggcgtca cagcccgacg aggatgccga gcccccagat ctgctcggtg
     4741 gcatcgacgc tgaagcgcgc ggcatccagg ccggcgtgat ggatgttgta gtccacgaag
     4801 atcgaggcgt tggcgatcat ggcatcgagc cggtactcga acccgccgcc gaccagatag
     4861 ccggtgccgg acaggtcgaa gccatcgctg ctctggttga gccaggttcg ttcgatcccg
     4921 ccgcgggcga atgcctcgaa gttgttgccc agcggcaggt tgagcttcag ggcgcccgag
     4981 gcctggtacg ccgtctgctc cccgaagcgg ctggtgaaga cgctgaatcc gttgagcgct
     5041 ccctccagcg acagcctgcc aaaccgcatg ccgacgagcc cgcgcaggct ccggccggaa
     5101 ggctgtccca ccgcctgcac gaacgcatcg ttgatccccg gctgcgatcc gagcgcgacg
     5161 cccaggtagg tatccgccga agccgtggag gagatcgcga agcacgccga ggtcaagaca
     5221 agcgcggtct tcatggcgag cttctgaagc actcctcgtg ccaccgccaa cccagtggat
     5281 tcacgggctc acgaggtagg tgctgttacc agctcttcac gcatccgggc acttagtggc
     5341 gcatccccgc agcggggcgc acccgcgcag ccacgatggc cggatacacc gtggcaccga
     5401 tgctgatcac gacgcccgcg gcggcgaccg ctgccaccga ccatgggtcg acgtgaaccg
     5461 gcagccggtc gatgtagtac acgttctcgt tgagcgggaa gccgtactcg ctcagcgccc
     5521 agcagccgcc cagaccggtg accacgccga gcgccgtgcc gatcaggccg atcatcaggc
     5581 cctggatcgc gaacagctgc atcacgccgg cgttgctcgc ccccagcgtc ttgagcagcg
     5641 cgatctcgcg cgccttctcg accacgacca tgatcaggtt gccgaggatc gagaacgacg
     5701 cgaccaggat cacgatgccg agcaccagga acatcgcgat cttctcgagc ttgagggccg
     5761 agaacaggct gcggttgagc tccttccagt cctggacccg gtaggcgggg ccaaacaccg
     5821 cctggaactc ggcgacgaac gcgtcgctgt catacgggtt gaccacccgg acctcgatgc
     5881 cgtcgatcgc gtcgccgcgg tcgaggaact cctgcagcga gtcgagcgtg acgtagacgt
     5941 acttgaggtc gtactcgtac attccggtga agaacgtgcc ggcgacccgg tagtcacggt
     6001 tgaaggggat cggcgtgccg ttcgcgtcgg ggttcgccgg atcggacagc ggcgacacca
     6061 cgcggatctc ctcgccggtg tagagatgga tctgcttgac cagctcgcgg ccgaccagga
     6121 tgccgggcag cgactgggtg cgccgcgaga tcgacggcgt gtcgaacggc gggtcgatca
     6181 ccttgaccgc gtcctgcagg tagtcgggct ggctgtaatc ggtcggcggc ccgtcggagg
     6241 tgaccaggtc gggcggtggc ggatcgatca cggcacccgg cacaccgccc gggcgctgga
     6301 gccgctgccc gaccctgcac cggaaccggc gctcgcggcc ggcgcgccgg agccatcgcc
     6361 ggccgggtcg cgcggccggg gtg
//
LOCUS       DASVOR010000342         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207794,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000342 DASVOR010000000
VERSION     DASVOR010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207794"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF516_07045"
     CDS             complement(<1..447)
                     /locus_tag="VF516_07045"
                     /inference="COORDINATES: protein motif:HMM:NF014816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8107468.1"
                     /translation="MKLYFAPGACSLSPHIVAQEAGIPLTLVKVDTQTKKLADGGDFW
                     TVNPKGYVPALQLDSGEVLTEGPAIVQYLADQKPAAKLVPPAGTFERSRVQEMLGYIN
                     SEIHKTYSPLFRPTTPAETRKEREEYLRKRYGLVEKQLAKGPYLFGD"
     gene            595..978
                     /locus_tag="VF516_07050"
     CDS             595..978
                     /locus_tag="VF516_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8107469.1"
                     /translation="MALPVRRNKVSSPLPACPVTTCMALLSGAWAPNVIWYLSAQPRR
                     FGELRVDIPRISARVLSARLRELESKGVVARRVMSTSPPSVEYELTELGRELLPAIDA
                     IIAVGTKLAMREGHERPRRRGREGG"
     gene            1009..>1856
                     /locus_tag="VF516_07055"
     CDS             1009..>1856
                     /locus_tag="VF516_07055"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEX8107470.1"
                     /translation="MTHRRFDRTARLLGDDGVARLASATVTVFGVGGVGASAAEALVR
                     SGVGRVILVDYDRICVTNVNRQLHAMKGTLGKPKAQVMAERLRLVNPDAVIEARAEFY
                     TADSSARLLVPEPDVVIDAIDHMAAKLHLIATCLRDRLRCVSAMGAAARLDPTAVRIA
                     DLSETRVDPFARDLRRNLRRKHGIDCTRPVGVWAVFSDEPPIAPRALAYDDGGFRCVC
                     PGGDNGLNDCEHRNRVEGSVAFVPSAFGAAAASLAVKLLCGLRLPRPARPLTETEAPR
                     PRRPTRL"
BASE COUNT          245 a          638 c          691 g          282 t
ORIGIN      
        1 gtcgccgaac aggtacgggc ccttggcgag ctgcttctcg accagcccgt agcgcttgcg
       61 caggtactcc tcgcgctcct tgcgggtctc ggccggcgtg gtcggccgga acagcgggct
      121 gtacgtcttg tggatctcgg agttgatgta gccgagcatc tcctggacgc gggagcgctc
      181 gaacgtgccg gcggggggca ccagcttggc cgcgggcttc tggtcggcga ggtactggac
      241 gatcgcgggg ccctcggtga gcacctcgcc gctgtcgagc tgcagcgcgg gcacgtagcc
      301 cttggggttg accgtccaga agtcaccgcc gtcggcgagc ttcttggtct gggtgtcgac
      361 cttgaccagc gtgagcggga tccccgcctc ctgcgcgacg atgtgaggag acagcgaaca
      421 ggctccggga gcgaagtaca gtttcatggg ttctctcttg gttctgcgtc ggttggactg
      481 cgacggccgc ggcatcgtcc gcgccgccgt gccgcacaac ctagcgcgcc gtgtatcggt
      541 tggatatatg ctaaccgtca gttactgaag attgtggtaa cctgggagta accaatggcg
      601 ctaccggtcc gccgcaacaa ggtgagctcg ccactcccgg cgtgcccggt cacgacctgc
      661 atggcgctgc tcagcggcgc gtgggccccg aacgtgatct ggtacctcag cgcccagccg
      721 cgccggttcg gcgagctgcg ggtcgacatc ccgcggatct cggcgcgcgt gctgagcgcg
      781 cggctgcgcg agctcgagag caagggggtg gtggcccgcc gggtgatgtc gacgtcgccg
      841 ccgtcggtcg agtacgagct caccgagctc gggcgcgagc tcttgccggc gatcgacgcg
      901 atcatcgcgg tcggcaccaa gctcgcgatg cgcgagggcc acgagcggcc gcgccggcgg
      961 gggcgcgagg gtggttgaca gcgccgcggc atcggcaaca atccggcggt gacgcaccgc
     1021 aggttcgacc gcaccgcccg cctgctcggg gacgacgggg tcgcgcggct ggcgagcgcg
     1081 acggtcaccg tgttcggggt cggcggggtc ggcgcgtcgg ccgccgaggc gctggtccgc
     1141 agcggcgtcg gccgcgtgat cctggtcgac tacgaccgga tctgcgtgac caacgtcaac
     1201 cgccagctcc acgcgatgaa gggcacgctg ggcaagccca aggcgcaggt catggccgag
     1261 cggctgcgcc tggtcaaccc ggacgcggtg atcgaggccc gcgccgagtt ctacaccgcg
     1321 gacagctcgg cgcgcctgct ggtccccgag cccgacgtgg tgatcgacgc gatcgaccac
     1381 atggcggcca agctgcacct gatcgcgacc tgcctgcgcg accggctgcg ctgcgtatcg
     1441 gcgatgggcg cggcggcgcg gctcgatccg accgcggtcc ggatcgcgga cctgtcggag
     1501 acccgggtcg acccgttcgc ccgcgacctg cgccggaacc tgcgccgcaa gcacgggatc
     1561 gactgcaccc gcccggtcgg ggtgtgggcg gtgttctccg acgagccgcc gatcgcgccg
     1621 cgcgcgctgg cctacgacga cggcgggttt cgctgcgtgt gcccgggcgg cgacaacggc
     1681 ctcaacgact gcgagcacag gaaccgcgtc gagggctcgg tcgcgttcgt gccgtcggcg
     1741 ttcggcgccg ccgcggcgtc gctggcggtc aagctcctgt gcggactgcg tctcccgcga
     1801 ccggcccggc cgctcaccga gaccgaggcg ccccggccgc gccggccgac gcggct
//
LOCUS       DASVOR010000343        10182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_3280,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000343 DASVOR010000000
VERSION     DASVOR010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10182
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3280"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VF516_07060"
     CDS             <1..651
                     /locus_tag="VF516_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107471.1"
                     /translation="TEASAPGPAGMPMPPPAPATPTPQLQGILTPTAPTPVAPGQWFA
                     FRALAMPGWDPKSVDVDAFLAWAIQVAKQTIADAELIRFDASGVYPDGHADLTLSRSG
                     VLMVQFISPSRARRDPSVPVGAQPDWHCMFHVMATVQTGPFIAPANGSSCEDVRPQRI
                     PRCSARGVWRQMIERKAPSGNAVAQIDYFAPDKARPAEWHGNITGAFSASFPDACP"
     gene            683..1264
                     /locus_tag="VF516_07065"
     CDS             683..1264
                     /locus_tag="VF516_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107472.1"
                     /translation="MLPRLGSSKLISTWIWVTVLISIAAALSGGLAGWLALAPARIWR
                     GEVWRLGTWVFVEPGALALILTCASIYKFGGELAPRWGERRLRRFMIEILGGAAVATT
                     LLALVSPEVWHRGYFGGWAACDVLVIAWARQYPDSELVLYGLLRLNGRNLIAVVIAVT
                     CVYALFAGPLAMAPELVACAAAYGYPGAWLARR"
     gene            complement(1312..2295)
                     /locus_tag="VF516_07070"
     CDS             complement(1312..2295)
                     /locus_tag="VF516_07070"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8107473.1"
                     /translation="MRDPSRSTSGVLHLPAPRLRRERDRTLKVWELASGRALAALEGH
                     TGEVNACAVTPDGRHVVSTSWDQTLKVWELASGRALTTLAGHTDVVTACTVTPDGQHV
                     ISASWDQTLKVWELATGRARATLEGFTTPTVEVDVVALKADAHPCLIAGAGIEAKAEH
                     RIMTPHLLAVGKGRQLEQVLEEHTAGGGLSLEGHAGWVTACAVTPDGRHVVSASQDKT
                     LKVWELASGRALATLEGHTGVVTACAVMPDGRHMVSVSWDQTLKVWAFDTYVCRLTHR
                     GDAGYLAVAATTTMIIAGDSAGSVWFLDARVLANGRPNEATSIKGIKKNPK"
     gene            3531..3707
                     /locus_tag="VF516_07075"
     CDS             3531..3707
                     /locus_tag="VF516_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107474.1"
                     /translation="MRKSIPKLALRKETVRTLGTMDLVRVLGGLDTDAAPVADSNPKA
                     CGATAAAVVAAPGA"
     gene            complement(3759..4241)
                     /locus_tag="VF516_07080"
     CDS             complement(3759..4241)
                     /locus_tag="VF516_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107475.1"
                     /translation="MRWMSYRGIVPDFVVVPDIVAGGPASLEWSAFWRDTVPDEFRAY
                     LAVQDGMTAADVVPHLPRYHGIFVGGSLAWKLATGGAWAELARRHGLGCHIGRVGTAV
                     RVHWARSIGATSVDSSLPLRAREHLDAFLAAVGPIRHAVACLDAIASSEGRPRAARSA
                     "
     gene            complement(4699..7056)
                     /locus_tag="VF516_07085"
     CDS             complement(4699..7056)
                     /locus_tag="VF516_07085"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8107476.1"
                     /translation="MKSDTWFRPVSARPLAGLRVGLPLLLLFHLLWVSDDILSLHGSR
                     GIIPWELTDLLRDPWVPGLPTLAKAFLPLGIGEHTAVLLLLSVYAASLLSLALGLHTR
                     LAALLAWGLHLSLMTSGSASYYGVDQLANTFLLYLLVFPSGRAWTFASRRACPQQEET
                     IPVGCLRVMQVHLGVIYLAAGLDKAMGSQWWNGEAIWQTVSQPIFSTFDLSWLARHPW
                     IPMVAGWSTLVVEIGYAFLIWPRRTRKVWSIATIGFHLGTCLFMGLVFFSSVMILLTS
                     CLFLIPEQVMERAVGPRRVLVPAALVVVIGVLSLGLAPRAEPDGDETGLSRDFARLVH
                     RVMVRDHIPGVAVGVVERGHIVYARGFGYRDVGKHLPVTTGTLFPLGSCSKAFTATAI
                     GLLVDEGRIALDAPVRTYLPDFSLEDPVASATLTTRDILTHRSGLPRHDLFWYQAPFS
                     RDELYQRLRFLEPSGPPRAQWRYNSLMFVVAGRIVEKVSGESWESFVQSRILVPLNMR
                     RTLLSAELMETDSDHAVPYALRDGSVQEIPMVTRLSAIAPAGGVQTSVNDLARWLTFH
                     ATRSPALLGEGMWRELHRPQAEMPAPDEPEVQHPYYALGWIHESYRGHPLVVHNGAID
                     GFTVHLGFLPETGQGLMIFMNRDLATTAVMAIAYSAYDRLLGLEPLDWEGRLKEMPEP
                     LQDVPDVALDFPIETVAGTYEHPAYGMLTIRANRDMLVMEFRTLRLTLVYQGERRFLS
                     RDPIATGAPQLSVRFSKPEIGEPLKLFVPLNFDEGDPVEVFTRVR"
     gene            complement(7053..7688)
                     /locus_tag="VF516_07090"
     CDS             complement(7053..7688)
                     /locus_tag="VF516_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107477.1"
                     /translation="MITTEAPRGRGATTEHTRRYATEEKRPPRGWIGRERDRFAHSRP
                     LGRLLVVGVLGCHLALVVAGAAHLTSRLHVPFGRGLRYYDALSGAGDSYSFFAPAVGP
                     QLRARFSLSTPHGDHSEETLETGKSREVGFRLGNLAGTVYVVAKRTDVRRAFLGALAA
                     SRLGAHPEVNRVQVTIEEWEMPTMAEYRVGARPRWRSLHEATFVRTSRTHP"
     gene            complement(7763..8014)
                     /locus_tag="VF516_07095"
     CDS             complement(7763..8014)
                     /locus_tag="VF516_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107478.1"
                     /translation="MKRKITMGLSILAVAAGVGWKLASHHETSAGLQDIRLIQGSEHS
                     HPGPTDALAGIRIRLSPSDFDDAVRATDHEVPQIAIAAE"
     gene            8343..8942
                     /locus_tag="VF516_07100"
     CDS             8343..8942
                     /locus_tag="VF516_07100"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8107479.1"
                     /translation="MICKKLAAMCIPLFISISAVVFSISAVVFADPPPSGRADTSERA
                     AKLSEAFSVLHAVSQWSTKMSEMADKKAKSDLVKDYARAMAAANANADTKLRSIAEKK
                     GVTIAPLNPQTEEGKSLLDRIKAETVLLGSLEGDAFDKEYMTLVTNTQQSVIHLLETT
                     KASAEDPEIKQVLDEMTTTVQNRLKLAQDIMAKIYGDKV"
     gene            complement(9615..>10182)
                     /locus_tag="VF516_07105"
     CDS             complement(9615..>10182)
                     /locus_tag="VF516_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107480.1"
                     /translation="CDRMPMTLDPVLRLARRCGVPPAAAAVRLMGVTVRFCAAVLSQD
                     GVIQWASPSLRLLGFMDVSRFPEGKPIGASALARRFVDTGRVRRDPDLVPASAWIDGV
                     SDDTWIQEHSMPTGQPGTVLTMLWAPNEPDVPRDLTLPPRLVPGVRDMYLSDEAGRIA
                     YHRAMISPCAIAPSWLDVLLGVNLRAAG"
BASE COUNT         1861 a         3427 c         3316 g         1578 t
ORIGIN      
        1 accgaggcca gcgcaccggg ccccgccggg atgccgatgc cgccccccgc gccggcgacg
       61 ccgacgccgc agctgcaggg cattctcacc ccgaccgcgc cgacgccggt cgccccgggg
      121 cagtggttcg cgttccgcgc gctggcgatg ccgggctggg atccaaagag cgtcgacgtc
      181 gacgcgttcc tcgcctgggc gatccaggtc gcgaagcaga cgatcgcgga cgccgagctg
      241 atccggttcg acgccagcgg cgtgtacccc gacggccacg ccgatctgac gctgtcgcgc
      301 agcggcgtgc tcatggtgca gttcatctcg ccgtcgcgcg cccggcgcga tccgagcgtg
      361 ccggtcggcg cgcaacccga ctggcactgc atgttccatg tgatggccac cgtgcagacc
      421 ggcccgttca tcgcgccggc gaacggcagc tcgtgcgagg acgtgcgccc gcagcggatc
      481 ccgcgctgca gcgcgcgcgg cgtgtggcgc cagatgatcg agcgcaaggc gccgagcggc
      541 aacgcggtcg cccagatcga ctacttcgcc cccgacaagg ccaggccggc ggagtggcac
      601 ggcaacatca ccggcgcgtt cagcgcgtcg ttccccgacg cgtgcccgtg atcgcgagcg
      661 acctcgcgct atgcttgcgc cgatgctccc ccgcctcggg tccagcaagc tgatctcgac
      721 ctggatctgg gtcacggtcc tgatatcgat cgccgccgcg ctgagcggcg ggctcgcggg
      781 ctggctggcg ctcgcgccgg cgcggatctg gcgcggcgag gtgtggcgcc tggggacctg
      841 ggtgttcgtc gagcccggcg cgctggcgct gatcctgacc tgcgcgagca tctacaagtt
      901 cggcggcgag ctcgccccgc gctggggcga acgccggctg cggcggttca tgatcgagat
      961 cctcggcggt gcggcggtcg ccacgacgct gctcgcgctg gtcagtcccg aggtctggca
     1021 ccgggggtac ttcggcggct gggcggcctg cgatgtgctg gtgatcgcgt gggcgcgcca
     1081 gtatccggac agcgagctcg tgctctatgg cctgctccgg ctcaacggcc ggaacctcat
     1141 cgccgtggtc atcgcggtca cctgcgtgta cgcgctgttc gccggcccgc tcgcgatggc
     1201 gcccgagctc gtggcctgcg ccgcggcgta cggatacccg ggcgcgtggc tggcgcgccg
     1261 ctgatcgacg gtcgaaatca ggggcgtgat atctacgctt aacaaccgaa attacttagg
     1321 attcttcttt attcccttga ttgaggtcgc ttcattaggg cggccgttag ccaggacgcg
     1381 cgcgtccaag aaccagacgc tgccggcgct gtctccggcg atgatcatgg tcgtggtcgc
     1441 cgccactgcg aggtagccag cgtcgccgcg atgcgtgagg cggcagacgt atgtgtcgaa
     1501 cgcccagacc ttgagcgtct ggtcccagga caccgagacc atgtgccggc cgtccggcat
     1561 caccgcgcac gcagtcacca cgccggtgtg gccttcaagc gtggcgagcg cgcgcccgct
     1621 cgccagctcc cagaccttga gcgtcttgtc ctgggacgcc gagaccacgt gccggccgtc
     1681 cggcgtcacc gcgcatgcgg tcacccaacc ggcgtgacct tcgagggaca gtccaccgcc
     1741 ggccgtgtgc tcctcgagta cctgctccag ttggcggccc ttcccaacgg cgaggagatg
     1801 cggtgtcatg atccggtgtt cggccttcgc ctcgatccca gcaccggcga tgaggcacgg
     1861 atgagcatct gctttcagtg caacaacatc tacctcgacg gtgggggttg taaagccttc
     1921 gagagtggcc cgcgcgcgtc cggttgccag ctcccagacc ttgagcgtct gatcccagga
     1981 cgccgagatc acgtgctggc cgtccggcgt caccgtgcac gcggtcacca cgtcagtgtg
     2041 gcctgcgagc gtggtcagcg cgcgcccgct cgccagctcc cagactttga gcgtctgatc
     2101 ccaggacgtc gagaccacgt ggcggccgtc cggcgtcact gcacacgcgt ttacctcgcc
     2161 ggtgtggcct tcgagcgcgg ccagcgcgcg cccgctcgcc agctcccaga ccttgagcgt
     2221 ccggtcccgc tcgcgccgga ggcgtggagc cggcaggtgc aggacgcctg acgtcgacct
     2281 ggacgggtca cgcatagtcg gcgaacagct tgtgctgctg cgccttcggc gcgggtgagg
     2341 atggcgcggc gagcgcagcg ctcggccgca tcgtcggcaa tggcagctcg ggcaccgtga
     2401 gccacccgac ctcgcgatcg agctcctcgc gatcgagccg agcgcgcgtg atgcgccagt
     2461 agcgcagagc gagctcgcgc ccaagtattg gcgcgccacg cgcggccgcc tcagacccga
     2521 ggagcccgcg cccccgctct ccgccttcga agtgctaccg cgagcgaccg acgagatggc
     2581 tgcgacgcct tccgccagcg ccgacgcgaa cccgtagccc gccggcctcg cgacgccagg
     2641 cgatgcgtcc cctgcgcccc gatccctaac agcaggctcc ggcacattgg ctccatcgaa
     2701 ggctcggcga ggacgaagcg acgaagccac tcacgggctc gctgtgtcgt ctcagctcgg
     2761 gcgtcaaagt cgagaacctc gatgcgcaca agagatagca attcgcggat gtcatcgggc
     2821 gtgggatccg tgcccatcgc aacgcgccat gacgtcgtga cgtgagactt cagcgtgcgt
     2881 agagcgttcg tctcgcccgc attggtcggg gcatcgtcga taggacggcc ggcgagtccc
     2941 ctgactttgg tcagaacgga agggacgtcc tcgatgaccg tatcaggcgt cctggcgccg
     3001 acgacgacaa caagtcgatc gcggttggga tcgagccgcc gttgccatgg cacagctgac
     3061 gaggtcgaca cacggtttgc caggaactga cgaacgaact gatcgatagc agaggctagc
     3121 cctgaagtaa gcgatcccag cgtgagtgca tagcgggaat ccagaccctc tgacggtggt
     3181 ttcgaggttg ctgacaccga gaagccccca ccggagaggt ccggaagtga aacctaccat
     3241 agaggtacga cgagcgagcc acgtgaacgc cggcaccgcg gtgcgccctt ccgagctacg
     3301 ttggtgacgc ggccccgccg ctcgtcattg acctcgaccg ctaccgcatc gtcgcgcagc
     3361 tcgcaggcaa tgccggccgg gtgttctcgg cgtgctgggt cgccgggacg cgcgtcctca
     3421 ccgcaggcgc cgacggcacg gcccggcgaa atctcacgct ggcgactgcc caaccccgcg
     3481 caggtcatcg ctgcctgcag tggccatcct ccctgcgcta tcttaccgtg atgcggaaat
     3541 caatccccaa gctcgcattg cgcaaggaaa cggttcggac gctgggcacg atggatctcg
     3601 tgcgcgtcct gggcggcctg gacacggatg ctgctccggt cgcggatagc aaccccaaag
     3661 cgtgcggagc aacagccgcg gccgtcgtcg ccgcaccggg cgcctgacgc gaagaaggtg
     3721 ctagcccttt cgcgatcgcg cacgcggcgt gcaccagctc acgcggaccg cgccgctcgc
     3781 ggccgcccct cgctggaggc gatcgcgtcc aggcacgcca ccgcgtgccg gatcggtcca
     3841 accgcggcaa gaaacgcatc caggtgctcg cgagcgcgca acggcagaga agaatcaacg
     3901 ctggtcgcgc cgatcgagcg cgcccagtgg acgcggaccg cggtcccaac gcggccgatg
     3961 tgacacccca ggccatgccg acgtgccagc tcggcccaag cgccgccggt cgccagcttc
     4021 caggccagtg atccgccgac gaagatcccg tgatagcgcg gtaggtgcgg aaccacgtcc
     4081 gccgccgtca tcccgtcttg caccgcgagg taggcgcgaa actcgtcggg cacggtgtcg
     4141 cgccagaacg ccgaccattc gagcgacgcg ggcccgccgg caacgatgtc cgggacgacc
     4201 acgaagtccg gcacgatgcc ccgataggac atccacctca tgtcgcgatc ccaacggcag
     4261 acgttgaacg gctgtcccgc tcgccagccc ccccgcgtag cgcaccagat ggcgacgcgg
     4321 gggcggtatg atcccgcaca gccggcgcag gaacgccacc ggcggtagca ccagctcggt
     4381 gcggccgtcg ggccacggcc gcttgagccg gtagccacgc gtcctgcaag ccgaggtcgc
     4441 ggtcaactac atcagccagg cgcctgggga tgcccaagtc gacggttgcg cattgggtca
     4501 agacggggaa gcttcccgcc ccgcgcttcc cggaagtccg agatcctgca cttgcccttt
     4561 ggcgacacgg atcgccatca cgaggatcac ggcgcagaca agtgcttcac ggttcactct
     4621 agcgttcgcg accggtgagt accatcgcgc gcacgcgctc gccacgacac gggcgctcgc
     4681 tgggctacat cgcgtcgcct acctcacgcg agtgaagacc tccacgggat cgccctcatc
     4741 gaagttgagc ggaacgaaga gcttcaacgg ctcgccgatc tccggtttcg agaaccgaac
     4801 cgagagctgg ggtgcgccgg tggcaatggg gtctcggctg agaaaccggc gttcgccctg
     4861 atacaccagc gtcaaacgaa gagtccggaa ctccatcacg agcatgtccc ggttggcccg
     4921 gatcgtgagc atcccgtagg cgggatgttc atatgtcccc gccaccgttt cgatcggaaa
     4981 gtcgagcgcg acatccggaa cgtcctgcaa cggttccggc atctccttga gtcttccctc
     5041 ccagtccagc ggttcgagcc cgagcaggcg atcataggcg ctgtaggcaa tcgccatcac
     5101 agccgtagtc gcgagatcgc ggttcatgaa gatcatgagc ccttgcccgg tctcgggaag
     5161 aaagcccaga tgcaccgtga atccgtcgat ggcaccgttg tggaccacca ggggatggcc
     5221 gcggtagctt tcgtgaatcc agcccaacgc gtagtagggg tgctggacct cgggttcgtc
     5281 cggcgccggc atttccgctt gcgggcgatg cagctcacgc cacatgcctt cccccagtag
     5341 cgcgggcgat ctcgtcgcgt gaaatgtgag ccagcgggcg agatcgttca cgctcgtctg
     5401 cacgcctccc gcaggcgcga tcgcgctcaa gcgcgtcacc atggggatct cttgtacgct
     5461 gccatcacgc agcgcgtatg gaacggcatg atcggagtcg gtctccatca gttctgccga
     5521 aagcagggtg cggcgcatgt tgagaggcac gaggatccgg gactgcacga agctctccca
     5581 gctctctccc gagaccttct cgacgattcg accggccacg acgaacatca ggctgttgta
     5641 tcgccactgc gcgcggggtg gaccgctggg ctcgaggaac cgaaggcgct ggtagagctc
     5701 gtcgcgcgag aatggggcct ggtaccagaa gagatcgtgc cggggtaacc cgctcctgtg
     5761 ggtgagaatg tcccgcgtcg tcaaggtcgc cgatgcgacc gggtcttcga gggaaaaatc
     5821 gggaaggtag gtgcggacgg gagcgtcgag cgcgatccgt ccctcgtcca cgagcagacc
     5881 gatcgctgtt gccgtgaatg ccttcgagca ggacccgagg gggaacagcg tgccggtcgt
     5941 taccggtaaa tgcttgccca catcgcggta accgaagccg cgggcgtaga cgatgtgccc
     6001 gcgttcgacg actcccaccg ccacgcccgg aatgtgatcg cgtaccatca cacggtggac
     6061 aaggcgcgcg aaatcgcggg aaagcccggt ttcgtcgcca tccggttcag ctctgggcgc
     6121 gagcccgagc gacagcacgc cgatcacgac gacgagcgcc gccgggacca gcacgcgcct
     6181 cggcccgacg gcacgctcca tgacttgttc gggaatcaga aagagacagc tcgtgaggag
     6241 aatcatcacg ctcgagaaga agacgagccc catgaacagg cacgtaccca ggtggaatcc
     6301 gatcgtcgcg atggaccaca ccttccgcgt gcgacgaggc cagatgagaa acgcgtagcc
     6361 gatctccacg accaatgtgc tccatcctgc aaccattgga atccagggat gccgcgccag
     6421 ccagctgaga tcgaacgtgc tgaagatggg ctgagagact gtctgccaga tggcctcacc
     6481 attccaccac tgactcccca ttgctttgtc tagaccggca gcgaggtaga tcacgccaag
     6541 gtggacttgc atgaccctca gacaaccgac gggaatcgtc tcctcttgtt gggggcaggc
     6601 ccgacgagac gcgaacgtcc aagcgcgtcc cgacggaaag acaagcagat acaagaggaa
     6661 cgtgttcgct agctgatcga ctccataata cgaagcagat ccgcttgtca tgaggctgag
     6721 atgaaggccc cacgccagca acgccgcgag tcgggtgtga aggccgagcg cgagcgagag
     6781 taggcttgcc gcatagacgg acagcagaag gagaactgcg gtgtgctcac cgatcccgag
     6841 gggcagaaag gccttcgcca gggttgggag gccaggcacc cagggatcgc gcagcagatc
     6901 cgtaagctcc cagggaatga tcccccgact gccatgcagt gaaaggatat cgtcggagac
     6961 ccagagcaga tgaaagagca gtagcaacgg gagccccacc cggagtcccg caagcggccg
     7021 agccgacacc ggccgaaacc aggtgtcgct cttcatggat gcgttctcga cgtgcgcacg
     7081 aaggtggcct cgtgtaagga acgccaccga ggccgcgcgc cgacgcggta ctcggccatc
     7141 gtgggcatct cccactcctc gatggtcacc tgcacgcggt tcacttcggg atgtgcgccg
     7201 aggcgactcg ccgcgagtgc gccaagaaac gcccgtcgca cgtcggtgcg cttggccacg
     7261 acgtacaccg ttccggcgag gttcccgagg cggaagccga cctcgcgact cttgccggtt
     7321 tccagtgtct cctcggagtg atctccatgc ggcgtagaaa ggctaaaccg tgcccgcaac
     7381 tggggcccta ctgccggagc aaagaacgaa taggagtcgc cagctcccga cagcgcatca
     7441 taatagcgca atcccctccc gaaaggcaca tgcaggcgcg acgtgagatg agcggcccct
     7501 gcgaccacga gtgcaaggtg gcaaccgaga actcccacca cgagcaaccg tcccaggggc
     7561 cgcgaatggg caaatcgatc gcgctcgcga ccgatccatc cccgcggggg tcgtttctcc
     7621 tcggtcgcgt accgacgggt gtgctccgtc gtcgcgcctc gcccgcgggg cgcctcggtc
     7681 gtgatcatcg atcgatttgc ccactcgcgg gcccttagcg ttcgcatgcg tcgctcctgg
     7741 cactgggccc agcatctcgg gcctattcag cagcgatggc gatttgcgga acctcgtgat
     7801 ccgtcgcccg gaccgcatcg tcgaaatcgc tgggactcaa gcggatccga atccctgcca
     7861 gtgcgtcggt cgggccaggg tgcgagtgct cggaaccctg gatgagcctg atgtcctgca
     7921 gccctgcgga ggtctcgtga tggctcgcga gcttccatcc cacaccggcc gccaccgcga
     7981 gaatggaaag acccatcgta atcttccgtt tcatgatgag ctcctcttgt tgcgacctgg
     8041 gaaagcaagt cccgatcccg ttcggcgcga gtcagcgcct gccgcagcga ccggacgtat
     8101 cgaagcgagc acactgatct ccgcggtctg gtagcagcta cgagccaacc agaagcagcg
     8161 acgaaaccgt gcgccccggc aaccgtgacc aagctcgcga gcgccctggc acgatctacg
     8221 acggcgatgt catcggaatc gcccatggaa cggaccgtgc aacaacgcgg tgaggcagga
     8281 tcgcggaaag gaaacacgag cagggaagct caacaggccg cgaacctcaa aaggagagac
     8341 tcatgatttg caagaaactc gcagccatgt gcatccccct cttcatcagc atcagcgcag
     8401 tggtgttcag catcagcgca gtggtgttcg ccgacccccc accctcgggg cgcgccgaca
     8461 cttctgagag agcggccaag ctgagcgagg ctttctcggt actgcacgcg gtcagtcagt
     8521 ggtccaccaa gatgagtgag atggccgaca agaaggcaaa gtcggatctc gtgaaagact
     8581 atgctcgcgc gatggcggct gcgaatgcga atgcggacac aaagctccgc agcatcgccg
     8641 aaaaaaaagg agtcaccatc gcaccgctga acccgcagac cgaggagggc aagagcctgc
     8701 tcgaccgcat caaggccgaa acggtgctgc tcggctcact cgagggggat gccttcgaca
     8761 aagagtacat gaccctcgtc acgaacactc agcagagtgt cattcatctc ctcgagacga
     8821 ccaaagcgtc ggccgaggat ccggagatca agcaggtcct cgatgagatg acgaccactg
     8881 ttcagaatcg cctgaagttg gcacaagata tcatggcgaa gatctatgga gataaagtct
     8941 aatcgacaag gcaccgacgt cgaggccagg acggcagcgc gtaaccgcgc ggggaacgat
     9001 cctgccgctc ggatctgatt tcagcgatcg ttcgcgccct tcgatcgagc ggttcgcgaa
     9061 gccgcacaaa gtaaacgtgt tcactattct tttccgtcca aaagtaaata cgttcacttg
     9121 tgatgaccca gggcgagcag acgcgacaac gcatcgtcga ccgagcgctc gagctgttcg
     9181 accccggatc gaagctgctc gacctccgcg cggcgcgcgt cgtcaatagc tcggtgaggt
     9241 ggagcggcgc gccctgtagc gcgttgtgca tcttctttgt gagcacgagc gtaccaactc
     9301 gcctgcgtac tcgtcgtggg ccgagcctcc aaccagcgcg tgatgaagcg atacggcaag
     9361 agctgatgcg cgctgttcga cgagctcgat agcagcccgc caagccgttc gcggcgcgac
     9421 cggcacgttg cggttggccg gtcgcatcgg atgctcggct ggcgtgagca gccggttgcc
     9481 cacgcccgcg tccctgacca gccggccgca tgccgtggcc gagcgccggc gcgccgcagc
     9541 acctcgcggc aaacgccagc ccccggccgg ctgccccggc gcggtgccag cgatcacgga
     9601 gggccatcga gcgactaccc ggcagcccgc aggttgacgc cgagcagcac atcgagccag
     9661 ctcggcgcga tcgcgcacgg gctgatcatg gcccggtggt acgcgatccg cccggcctcg
     9721 tcgctcaagt acatgtcgcg gacgcccggc accaggcgcg gcggcagcgt gagatcgcgc
     9781 gggacgtccg gctcgttggg cgcccacagc atcgtcagca ccgtcccggg ctgacccgtc
     9841 ggcatggagt gctcctggat ccacgtgtcg tcgctcacgc cgtcgatcca tgcgctggcg
     9901 ggcacgaggt ccggatcgcg gcggactcgt ccggtgtcca cgaaccgacg cgcgagggca
     9961 ctcgcgccga tcggcttgcc ttcggggaag cgcgatacgt ccatgaaccc cagcaagcgc
    10021 agcgacgggg aggcccactg aatcacgcca tcctgggaca gcacggcggc gcagaaccgg
    10081 accgtcacgc ccatcaggcg aaccgcggcg gcggccggcg ggaccccaca gcggcgggcg
    10141 agccggagca cggggtcgag cgtcatcggc atccgatcgc ag
//
LOCUS       DASVOR010000344         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_353879,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000344 DASVOR010000000
VERSION     DASVOR010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353879"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VF516_07110"
     CDS             complement(<1..1466)
                     /locus_tag="VF516_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEX8107481.1"
                     /translation="MEDPNGQLARALPLIRALQHKAEQLEAERIEPIAVVAMACRLPG
                     DVASPAELWRLLAEGRDAVTELPRRWDEWDLRGTGVDEPGTSYCRHGGFLHDVERFDA
                     GFFGITPREAEAMDPQQRLALEVCWEALERAGITMQAARGSAIGVFLGAMNTDYAMAT
                     RSDLAALDGYHGTGSASSVLSGRVSYALGLEGPALTVDTACSSSLVAIHLACAALRRG
                     ECRAALAGGVTVMSTPATFVEFSRIKALARDGRCKSFAAEADGVGWADGAATLLLKRL
                     STAVEDGDRVLAVIRGSAVNQDGKSQGLTAPNGPAQERVIRAALDACRLAPDAIDAIE
                     AHGTGTPLGDPIEARALARVLGRDRAAHRPVWLGSVKSNLGHTQAAAGAVGMIKLVLA
                     LDHELLPPTLHAGTPSPHIDWPDSGLALLVNAVGWPRGNRVRRAGVSSFGISGTNAHV
                     IVEEPSRAEPPTATVRATTAYPVLISARTRAALARQADR"
     gene            complement(1469..>1831)
                     /locus_tag="VF516_07115"
     CDS             complement(1469..>1831)
                     /locus_tag="VF516_07115"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8107482.1"
                     /translation="LGMAPHELALEQTLSEVGLDSMLAVELRRRLAAATAVALPATLA
                     FDYPSASAIAGLLLEELGLDDRRANPSGTVAAGRIALGAGGMLPAGAPRDGLATAASV
                     HELSAELDALLGVVGLES"
BASE COUNT          226 a          685 c          692 g          228 t
ORIGIN      
        1 cggtccgcct ggcgcgcgag cgcggcgcgg gtccgcgcgg agatcaggac cgggtacgcg
       61 gtggtcgcgc gcacggtggc ggtgggcggc tcggcgcggg agggctcctc cacgatcacg
      121 tgcgcgttgg tgccgctgat cccgaacgac gacacgccgg cgcggcggac gcggttgccg
      181 cgcggccagc cgaccgcgtt cacgagcagc gcgaggccgc tgtccggcca gtcgatgtgg
      241 gggctcggcg tcccggcgtg cagcgtcggc ggcagcagct cgtggtcgag ggcgaggacg
      301 agcttgatca tgccgacggc gcccgccgcg gcctgggtgt ggccgaggtt cgacttcacg
      361 cttcccagcc acaccgggcg gtgcgccgcg cggtcgcggc cgagcacgcg ggcaagggcg
      421 cgcgcctcga tcggatcgcc gagcggcgtg cccgtgccgt gcgcctcgat cgcgtcgatc
      481 gcgtcgggcg cgagccggca tgcgtcgagg gcggcgcgga tcacgcgctc ctgggccggg
      541 ccgttcggcg cggtcaggcc ctggctcttg ccgtcctggt tgaccgcgga gccgcggatc
      601 accgcgagca cgcgatcgcc gtcctcgacc gcggtcgaca accgcttgag caggagggtc
      661 gccgcgccgt cggcccagcc gacgccgtcc gcctcggccg cgaagctctt gcaccggcca
      721 tcgcgggcga gcgccttgat ccggctgaac tccacgaacg tcgccggcgt gctcatcacg
      781 gtgacaccac cggcaagcgc ggcgcggcac tcgccgcggc gcagcgcagc gcacgcgagg
      841 tggatcgcga ccagcgacga cgagcatgcg gtgtcgacgg tcagcgccgg gccctcgagc
      901 ccgagcgcat acgacacccg gccggacagc acgctcgagg cgctgccggt gccgtggtag
      961 ccgtcgagcg ccgcgaggtc gctgcgggtg gccatcgcgt agtccgtgtt catcgcgccc
     1021 aggaacacgc cgatcgcgct tcctcgcgcg gcctgcatcg tgatcccggc gcgctcgagc
     1081 gcctcccagc acacctcgag ggcgaggcgc tgctgggggt ccatcgcctc ggcctcgcgc
     1141 ggcgtgatcc cgaagaagcc tgcgtcgaag cgctcgacgt cgtggaggaa gccgccgtgc
     1201 cggcagtagc tcgtgccggg ctcgtccacc ccggtgcccc ggaggtccca ctcgtcccac
     1261 cgccgcggca gctcggtcac cgcgtcgcgg ccctcggcga gcaggcgcca gagctccgcg
     1321 ggactcgcga cgtcgcccgg cagccggcac gccatcgcga ccaccgcgat cggctcgatg
     1381 cgctcggcct cgagttgctc cgccttgtgc tggagggcgc ggatgagcgg gagcgcccgc
     1441 gcgagctgac cgttcggatc ttccatcgtc atgactccag ccccacgacc ccgaggaggg
     1501 cgtcgagctc cgcgctgagc tcgtgcacgg acgcggcggt cgccaggccg tcgcggggcg
     1561 caccggcggg cagcatgccc ccggctccga gagcgatccg ccccgccgcg accgtgccgc
     1621 tggggttcgc gcggcgatcg tcgagaccga gctcctcgag cagcaacccg gcgatcgccg
     1681 aggcgctcgg atagtcgaac gcgagcgtcg cgggcagcgc gaccgcggtc gcggcggcaa
     1741 gccgccgtcg cagctcgacc gcgagcatcg agtcgagccc gacctcgctg agcgtctgct
     1801 ccagggcgag ctcgtgcggc gccatgccga g
//
LOCUS       DASVOR010000345         6189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_383035,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000345 DASVOR010000000
VERSION     DASVOR010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6189
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383035"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VF516_07120"
     CDS             <1..999
                     /locus_tag="VF516_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107483.1"
                     /translation="RILAISAYPFLTHDDTGVDELGKIAADNNADAGLRQAAATSFAL
                     LAHDAKDITILQGLAQKYLDASAKKRAEADGKPKAVADAADKEFAKAKKLYDDAKARV
                     DRVSHDNTKTAADIREATDAASKASDAFKAAKKAHGDAIAPYKAADIAAKTYKSFARL
                     FQSHIARIEVAIRCRQDINCYAGTLQQKPADAAKSSASYIRDIKDWTADEQLSLVEGS
                     VERAMLEVGKRGQKASALTDKLLDSAKSDNRIVRQSVLLALPKIAAVPCANCEAKLQD
                     AIRAGEGKTTLGDLNLETTMMKNYFGWAGGKTPSSAPVEKDDLPAPSAPAPPPRKK"
     gene            1039..1115
                     /locus_tag="VF516_07125"
     tRNA            1039..1115
                     /locus_tag="VF516_07125"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1073..1075,aa:Pro,seq:tgg)
     gene            2009..2635
                     /locus_tag="VF516_07130"
     CDS             2009..2635
                     /locus_tag="VF516_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107484.1"
                     /translation="MATAGGLAAEILPPADRSRYGKARILVLGERYIGQRRSRSDVVL
                     ARDIFTSAVRTSNGVARRRCHVFCSDLRKQMMTTLRFMERYRVASTRAWIVCAAIATS
                     GLGCGGEVEPAVAASPPAATTATAGGPWTNQPFTEQTQIFHPVFAATPSASSIDAVVG
                     LSDGPAADFTDLAAIVRFNPDGTIDVRSGPARARLPVPHRAGGRGAPE"
     gene            2954..4156
                     /locus_tag="VF516_07135"
     CDS             2954..4156
                     /locus_tag="VF516_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107485.1"
                     /translation="MYPELGSNLVHVIADVTSHTYSVLTEGFVRIARQYQFRTQQLGV
                     THLQQLSAIVDGPEGSVAVCGLSDWASSDLVYSREDVVYKAIVPLANNEALLDDGETV
                     SRVDAGGRTVAQIPSGGKIAADAAGNVFVADVADTTLTIDKYDPGLAPRWRTTQAVPA
                     GTAVQAIASDAYGAVVIALTAPEDPNQAVLRFTADGAFASQASVQRGAVALDGDQPVV
                     AWSESDQVRVARFSATGEVVWSRSFAGRAAVEVIAVDPFHNVVFGGELATEVDFGGGP
                     LPLLEDDLHALHTFVVKLSASGDHVFSKANGLWRQLTAIASNGARVVVTGRSPGGAAP
                     EPRLQAFDAAGNQIFLGGSVAKFEQGFGGVDALAIGASGRIWWNLHFRDMSPSFFAEL
                     PYLLVLHE"
     gene            4383..5183
                     /locus_tag="VF516_07140"
     CDS             4383..5183
                     /locus_tag="VF516_07140"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HEX8107486.1"
                     /translation="MKQNGNRAEEETDDDLALLLAPGTSLGGARPKASVRDPGGRLAI
                     AKFPSPQDEWPITRWEAATLAMAESAGIEVPRRQLATVLKKPVLILERFDRRGSERIP
                     YMSALTAMAARDGDTRSYLEIAEVLQSSGGAPARDLDQLWRRVVFNVLVSNVDDHLRN
                     HGFLHEPGGWRLAPAFDLNPVPTDVRPRVHALAIDEDDPGAPLLRVLAIAPQFNVKAA
                     PARAIVREVAAVTRRWRRFASDCGLTQRQLDRMATAFEHDDADAARAL"
     gene            complement(5180..>6189)
                     /locus_tag="VF516_07145"
     CDS             complement(5180..>6189)
                     /locus_tag="VF516_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107487.1"
                     /translation="TVGELADQYRFFHWHLQFPQVFAKGGFDVVLGNPPWDTLSPDAK
                     EFFSIYEPGIRFQSKGDQDKTISRLLEDAGVAAAWERNRREIFASAFFLKESGRFTLF
                     APGHLGKGDFNLYRLFVELALRSVQNRGFAAQIVPDGLYAGANSMAIRKQLFESFRLD
                     LLLGFENAKEVWFPGVDSRAKFSLYAAQNGETTDVFRAAFCIRSPDTPIGTKLVESAL
                     RLTCTGPEMNGLWDEFANLGTVPPRAQGQVPGAIDATERLNLRADIEARLALLYGLSV
                     EEVGHVLDTFPIVQKADEQACGEFRTKVLVLHRYEELSRPSGGGAVPLRHLAKPEIVE
                     R"
BASE COUNT         1015 a         2150 c         2107 g          917 t
ORIGIN      
        1 cggatcctcg cgatcagcgc gtacccgttc ctgacccacg acgacaccgg ggtcgacgag
       61 ctgggcaaga tcgcggccga caacaacgcc gacgccgggc tgcgccaggc cgccgcgacc
      121 tcgttcgcgc tgctcgccca cgacgccaag gacatcacca tcctgcaagg cctggcccag
      181 aagtacctcg acgcctcggc caagaagcgg gcggaggccg acggcaagcc caaggcggtc
      241 gccgacgccg ccgacaagga gttcgccaag gccaagaagc tgtacgacga cgccaaggcc
      301 cgggtcgacc gggtgtcgca cgacaacacc aagaccgcgg ccgacatccg ggaagcgacc
      361 gacgccgcca gcaaggcctc ggacgcgttc aaggccgcca agaaggcgca cggcgacgcg
      421 atcgcgccct acaaggcggc cgacatcgcg gcgaagacct acaagagctt cgcgcggctg
      481 ttccagagcc acatcgcgcg gatcgaggtc gcgatccgct gccggcagga catcaactgc
      541 tacgcgggca cgctgcagca gaagcccgcc gacgcggcga agtccagcgc gagctacatc
      601 agggacatca aggactggac cgccgacgag cagctctcgc tggtcgaggg cagcgtcgag
      661 cgcgcgatgc tcgaggtcgg caagcgcggc cagaaggcgt cggcgctgac cgacaagctg
      721 ctcgacagcg ccaagagcga caaccggatc gtccgccaga gcgtgctgct cgcgctgccc
      781 aagatcgccg cggtaccgtg cgccaactgc gaggccaagc tccaggacgc gatccgcgcc
      841 ggtgagggca agaccacgct gggcgatctg aacctcgaga ccacgatgat gaagaactac
      901 ttcggctggg ccggcggcaa gacgccgtcg agcgccccgg tcgagaagga cgacctgccg
      961 gcgccgtccg cgccggcgcc gccgccgcgg aagaagtagt ggcggatcgc cgcgcggtgg
     1021 tgtaagcctc gcaggcatcg gggcgtagcg cagcctggta gcgcacctgg tttgggacca
     1081 gggtgtcgga ggttcgaatc ctctcgcccc gaccacggtt tctcgacgag gattcgggga
     1141 tcctcggcct gcctgggcaa cgtgttgccc acctgttgcc caaacgcacg ctcgatggcg
     1201 ttccgcttgt cggcggacgt caccgtgacg tagcgctgag tcgtcttcat gtcctcgtgc
     1261 cccatgaggt cgcgcagggt catgaccggg atgccccggc gagcgcactc ggaaccgaac
     1321 gtgtgccgca ggctgtggaa cggcatcgtc cgccccgtct cggacctggg cacgaccaca
     1381 ccggcgcgct tgtacaggcg ccggatggcc tggagcatcg tccggtagac caggaacgat
     1441 ccatcgtccg agctgagacg atgatgggcg acagaatcat gtgccgcagg ctcttctcgc
     1501 cgaagccgcg ccccgtagcg tgcctctaat tgtctcccca ccgcagagac aatctgggcc
     1561 ccgtactccg ctcggcgctc ttcgagcacc accgtgtgga cgcgctggcc gatctgccag
     1621 tacagcccgg tcagcgcggc gttcgcggta accgacacct gcttgcgcgc ggcgtcgatc
     1681 atcgcgccga tgtcgcgcac gagcgtcgac acctcggcct ggacgtgggc gccggacaca
     1741 cgtgcggcct tctccgcagc ggcgcccggc aactgtcgga gcgccgccgc gtcccccctt
     1801 gagctgctgg cttcggggtt gcgggttcca gaaattgaac gtcctcggcg cgaagggacc
     1861 gggaaggaga ggacaccctc atgccgccgg acccgcgata cctgcctcgg tgccgcgagc
     1921 cacccgcggc ggacgaggcg gtacgtcgcc gcacgtactg accacgaccg ccctgctgcc
     1981 agcgccgacg gcggccatgc cggcgctcat ggcgaccgcc ggcgggctcg cagccgagat
     2041 cctgccgccg gccgaccgct cgagatacgg aaaggcacgt attctcgttc tcggcgaacg
     2101 atacatcggc cagagaagat cacgcagcga cgttgtgctc gcacgcgata tttttacgag
     2161 cgctgtccgc accagcaacg gagtcgcgcg ccggcgctgc catgtttttt gctccgatct
     2221 gcgcaaacaa atgatgacaa cactgcgctt catggaacgc tatcgagttg cgagtacccg
     2281 ggcatggatc gtgtgcgccg cgatcgcgac atccgggctc ggctgtggcg gcgaggtcga
     2341 gccggcggtc gccgcgtcgc cgccggccgc gaccaccgcg accgccggcg ggccctggac
     2401 caatcagccg ttcaccgaac agacccagat cttccacccc gtgttcgccg ccacaccgtc
     2461 ggcgagctcg atcgacgcgg tggtcgggct cagcgacggc cccgccgccg actttaccga
     2521 tctcgccgcg atcgtccggt tcaaccccga cggaaccatc gatgtgcgct cgggtcccgc
     2581 tcgcgcacgg ctacccgttc cgcaccgagc aggcgggcgc ggcgcgcctg agtaacctgg
     2641 cgagcaaggt cgattcgccg accggcacgc tcgacgttcc cggggtcggg atccacacgg
     2701 atcccacgac catgaccgga tgtctggtcg cccgggccgg cgacgggttc gtgaccaggg
     2761 aggtggccga cacaaccgcg ctgagaatca ccagcttcaa cctcttcgcg agcgcgccgg
     2821 acctcgacgc cgtgatcggg ctgtccgcgg gtcggcctgc gagcttctcg gccctggcta
     2881 ccgggtccgc ctggcgcccg gcagcttcat cgacgtacgc gacggcgacg tgtaccgggc
     2941 cgaccgcacg gtcatgtacc cagagctcgg ctccaacctc gtccacgtga tcgcggacgt
     3001 cacgtctcac acctactcgg tgctcaccga ggggttcgta cggattgctc gccagtacca
     3061 gttccgcacc cagcagctcg gggtgaccca cctccagcag ctgtccgcga tcgtcgatgg
     3121 ccccgagggc agcgtcgcgg tctgcgggct ctctgactgg gcctcgagcg acctcgtgta
     3181 cagccgggag gatgtggtgt acaaggcgat cgtaccgctg gcgaacaacg aggcgctgct
     3241 cgacgatggc gagacggtga gccgcgtcga cgctggcggc cggaccgtcg cgcagatccc
     3301 cagcggggga aagatcgcgg cggacgccgc cggcaacgtc ttcgtcgccg acgtggcgga
     3361 cacgacactg accatcgaca agtacgatcc gggcctcgcg ccacgctggc gcaccaccca
     3421 ggctgtgccc gcgggcaccg cggtccaggc gatcgcgagc gacgcgtacg gcgccgtggt
     3481 gatcgcgctc accgcgccgg aggacccgaa ccaggcggtg ctgcgcttca ccgccgacgg
     3541 cgcgttcgcg tcgcaggcgt cggtccaacg cggcgccgtt gcgctcgacg gcgaccagcc
     3601 ggtcgtggcg tggagcgaat ccgaccaggt ccgggtcgcc cggttctcgg cgaccggcga
     3661 ggtcgtctgg agccggtcgt tcgccgggcg ggcggcggtc gaggtgatcg ccgtggaccc
     3721 gttccacaac gtggtgttcg gaggcgagct cgcgaccgag gtcgatttcg gcggcggacc
     3781 gctgccgttg ctcgaggacg acctccacgc tttgcacacc ttcgtggtca agctgtcggc
     3841 cagcggagac catgtgttct cgaaggcgaa cggcctgtgg aggcagctca ccgcgatcgc
     3901 gtcgaacggg gcgcgcgtgg tggtgacggg caggagtccg ggcggagcgg ctcccgagcc
     3961 gcgcctgcag gcgttcgacg ccgccgggaa ccagattttt ttgggggggt cagtcgccaa
     4021 gttcgagcag ggcttcggcg gcgtcgatgc cctggccatc ggcgcctcgg ggcgcatctg
     4081 gtggaacctg catttcaggg acatgtcacc gtcgtttttt gcagagctgc cctacctgct
     4141 ggtgctgcac gaatgagcca cggcatcgct ccgagcgaga catcgacgtg aacgccgttg
     4201 cttgcaacga ggaggagcgt cctgtgtcgc agcgcgaacg agcgcgcatc ggccagccgg
     4261 ccggctaacg ccccgagcaa cacgtcgata tgccgctcct cgtcgccgac ccggtgatca
     4321 cggtgaggtc gatatcggca gtcatcctgg gttgtcccca gcgttgcacg gcgatgcccc
     4381 cgatgaaaca gaacgggaac cgggccgagg aggagaccga cgacgacctc gcgctgctcc
     4441 tggctcccgg gacctcgctc ggcggcgcgc gccccaaggc atccgtgcgc gatcccgggg
     4501 gcaggcttgc gatcgcgaag ttcccgtcgc cccaggacga gtggccgatc acccgatggg
     4561 aggccgcgac cctcgcgatg gccgagtccg ccgggatcga ggtcccccgg cgccagcttg
     4621 cgaccgtgct gaagaaaccg gtcctgatcc tcgagcgctt cgaccggcgt ggctccgagc
     4681 gcatcccgta catgtcggcg ctcacggcga tggccgctcg cgatggagac acccgtagct
     4741 acctcgagat cgcggaggtc ctgcagagct ccggcggggc gcccgcgcgc gatctcgatc
     4801 agctgtggcg gcgcgtcgtg ttcaacgtgc tggtctcgaa cgtcgatgat cacctgcgta
     4861 accacggctt cctgcacgag cccggcggat ggcggctggc acccgcgttc gatctcaacc
     4921 ccgtcccgac cgacgtccgg ccgcgcgtcc acgcgctcgc gatcgacgag gacgacccgg
     4981 gcgcgccgct tctgcgcgtg ctcgcgatcg caccgcagtt caacgtcaag gcggcgccgg
     5041 cgcgcgcgat cgtccgcgag gtcgccgccg tgacccgccg ctggcgccgg ttcgcgagcg
     5101 actgcggcct cacgcagcgc cagctcgacc ggatggcgac cgcattcgag cacgacgacg
     5161 ccgacgcggc gcgtgcgctc tagcgctcga cgatctccgg cttcgccagg tggcgcagcg
     5221 ggaccgcccc gccaccactc ggccgggaca gctcctcgta ccgatggagg acgaggacct
     5281 tggttcgaaa ctcgccgcag gcttgctcgt cggccttctg gacgatcggg aacgtgtcga
     5341 gcacatggcc gacttcctcg acgctcaacc cgtagaggag cgcgagtcgt gcctcgatgt
     5401 cggcgcgcag gtttaggcgc tcggtcgcgt cgatcgcgcc aggtacttgc ccctgggctc
     5461 gcggtgggac ggttccgagg ttggcgaact cgtcccagag cccgttcatc tcggggcccg
     5521 tgcacgtgag ccgcagcgcg gactccacca gcttggttcc aatcggagta tcgggcgacc
     5581 ggatgcagaa cgcggcgcgg aagacgtcgg tcgtttcgcc gttctgcgca gcgtagagcg
     5641 agaacttcgc tcgtgagtcg acgccgggga accacacctc cttggcgttc tcgaacccga
     5701 ggagaaggtc gaggcggaag ctctcgaaca gctgctttcg gatcgccatg ctgttggcgc
     5761 cggcgtagag gccgtccggt acgatctgcg cggcgaagcc acggttctgg accgaccgga
     5821 gcgcgagctc gacgaacagc cggtacaggt tgaagtcgcc cttgccgagg tggccgggcg
     5881 cgaacagggt gaaccgaccg gattccttca ggaagaaggc ggacgcgaag atttcacgac
     5941 gattccgctc ccatgcggcc gcaacgccgg cgtcctcgag cagccgggag atggtcttgt
     6001 cctgatcgcc cttggattga aagcggatcc ccggctcgta gatcgagaag aactccttgg
     6061 cgtcggggct cagcgtgtcc cacggcgggt tgccaaggac gacgtcgaag ccgcccttcg
     6121 cgaacacctg cgggaactgc aggtgccagt ggaagaaccg atactggtcc gcgagctcgc
     6181 cgaccgtct
//
LOCUS       DASVOR010000346         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_470851,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000346 DASVOR010000000
VERSION     DASVOR010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_470851"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VF516_07150"
     CDS             <1..278
                     /locus_tag="VF516_07150"
                     /inference="COORDINATES: protein motif:HMM:NF023180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HEX8107488.1"
                     /translation="APARWDGSARRAARAPPSAAPAAPGGSPDGPMAVGIGIAISENM
                     LLTILIILLLLAALGGGFGYSRYGYGSWSPAGIIVVILIVMLLTGRL"
     gene            complement(468..1439)
                     /locus_tag="VF516_07155"
     CDS             complement(468..1439)
                     /locus_tag="VF516_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107489.1"
                     /translation="MTTAPPKPKTFAASSLALPGAAPYVLMDYIVFNPRTKTVWAPAG
                     NTAAVDVVDVATRHVAQVTGFATREIERNGQKRTIGPSSAAIGAPGTVYVGNRGDSSV
                     CAVDEAKLALGTCGKLDSSPDGLIYVAKTHEVWVTTPRDNSIRILDGTTLAQKAKLTL
                     EGNPEGFAVDATRNRFYTNLEDKDQSLAIDLTSHAVVATWKTACGEGGPHGIRLAEPE
                     GFLLLACPAKLEVLDVGHDGAIVGTADTGEGVDDIDYVPASHLVYVGAGRAGKLTIVW
                     FDAKGALTPIAVVPTKQGARNPAAASDGSIYLQNGQAGELVVVSPTS"
BASE COUNT          243 a          578 c          737 g          277 t
ORIGIN      
        1 gggcgccggc gcgctgggac ggatctgcac gacgcgcggc gcgtgcgccg ccgtccgccg
       61 cgccggcggc gcctggtggt agtcccgacg ggccgatggc cgtgggaatc ggaattgcaa
      121 tctcggaaaa catgctgctc accatcctca tcattcttct cctcctcgcg gcgctcggcg
      181 gtggcttcgg atactcgcgc tatggatacg gaagctggtc gcctgcgggg atcatcgtcg
      241 tgatcctgat cgtgatgctg ctgaccggcc ggttgtaacg gcgtcggtcc tgggcgaaga
      301 tcgccgcgga tgccgagccc gcggccgagc ggctgacgcg ctgttcgccg aggcgatcgc
      361 gctgacgccc aacggccgcg ccgcgctgct cacggcggac gcgacggcgg tcatccgttc
      421 ctgatcatgg aatccatcga cagcctcgcc gtggcgggcg aggctgctca gctcgtcggg
      481 ctgacgacca cgagctcgcc ggcctggccg ttctgcaggt agatgctgcc gtcggacgcc
      541 gcggcggggt tgcgggcgcc ctgcttggtc gggacgaccg cgatcggggt gagcgcgccc
      601 ttggcgtcga accagacgat cgtgagcttg ccggcgcggc cagcgccgac gtagacgagg
      661 tggctggccg ggacgtagtc gatgtcgtcg acgccctcgc cggtgtcggc ggtgccgacg
      721 atcgcaccgt cgtggccgac gtcgaggacc tcgagcttgg cggggcaggc gagcagcagg
      781 aagccctcgg gctcggcgag ccggatgccg tgcgggccgc cctcgccgca tgcggtcttc
      841 caggtcgcga cgacggcgtg gctggtgagg tcgatcgcga ggctctggtc cttgtcctcg
      901 aggttggtgt agaaccggtt gcgggtggcg tcgaccgcga agccctcggg gttgccctcg
      961 agcgtcagct tggccttctg tgccagcgtg gtgccgtcga ggatgcggat cgagttgtcg
     1021 cgcggcgtgg tgacccagac ctcgtgggtc ttggcgacgt agatcaggcc gtcgggcgac
     1081 gagtcgagct tgccgcaggt gccgagcgcg agcttggcct cgtcgaccgc gcacaccgag
     1141 gagtcgccgc ggttgccgac gtagacggtg cccggggcgc cgatcgcggc cgagctcgga
     1201 ccgatcgtgc gcttctggcc gttgcgctcg atctcgcggg tggcgaaccc ggtgacctgc
     1261 gcgacgtggc gggtggcgac gtcgacgacg tcgaccgcgg cggtgttgcc ggcgggtgcc
     1321 cacacggtct tggtccgcgg gttgaagacg atgtaatcca tgagcacgta cggcgcggcg
     1381 ccgggcagcg cgagcgagct ggccgcgaac gtcttcggct tggggggcgc cgtggtcacc
     1441 gccgggaccg ggcccgatac gggcggtgca gaaggcgcgc tgtggcagcc tgaggcggcg
     1501 aggcagaggg tcagaccgag accgagaccg agaccgagac cgagaccgag atcgagatcg
     1561 agatcgagat cgagatcgat gcgaggaggc atgtcgagct ccgtgggaag gggcggtcgg
     1621 gaaccgcgcc gccgggccgg ttatacgcgc gggcgcacgg tgcgggatag agggcgggcc
     1681 ggggcggaac gcccctgtgc ggtgcggcta cggcggggcg agcgggaggc ggagcgagaa
     1741 ccgcgcgcca ccggtcggtc ggttggacgc ggcgatcacg ccgccgtggc gctcggcgat
     1801 ccggcgtgcg atcggcaggc cgaggccgag gccgg
//
LOCUS       DASVOR010000347         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_178681,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000347 DASVOR010000000
VERSION     DASVOR010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178681"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1986)
                     /locus_tag="VF516_07160"
     CDS             complement(<1..1986)
                     /locus_tag="VF516_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107490.1"
                     /translation="MSWPPAYDYRLSYLDLANEVPALGAGLHAELEALRGAIDAEWKR
                     CHALGGNPAARMQDAATIARCLRRIAELLPGEADRRALGLRADAVERGHDNDTLAALA
                     AVDEDITIVAGKPVTWYGKPAGGLPTAFGCVRDTASQASVTAARARLGDVTAYLLGLH
                     EYLRLGEIPAFTATRLFFMAGEGDRHPKHIAYFIPEDEGMERCPFLKTYYFANTHRAL
                     IDAVSLPLGRRLLNLGLRLPASAASFGAIPVLGVLAHQFGRSVLRPGAFAALDTADRW
                     ASYVLQETAAGVFGTLVLAELLAPRLGLEPSDVVAYHLAECLRYVDRGLGNFPDSDGA
                     YLQLNYLASSGALVLDGGMPRLTGDPAAVILGFRSMARVLADTLLAGQIHDALAFYRA
                     YGPAGSHALHPLLVALREEPPKTIEYLQESRITARAREASQPTAPATNPARDTMRDFY
                     CRPVGDTALNRYEVWERGEAVGDSVTPSSYCPEYRMHMALKILSLPRPHERVFSIGCG
                     NAFVEADLQARGLPVQAIDCIEEAVALAAAKGVDAFTADYHTLPPGHLARFGVVYADG
                     LIGHLYHPETGLTGFFETLLALKPPPGSWLVLSNDAPAEPGAGVTPHGKVPGFWLFSP
                     EYLRGVAQRFGYTVLEAYSFPYERPLSGLRNRTICVAR"
BASE COUNT          314 a          713 c          767 g          294 t
ORIGIN      
        1 gcgcgcgacg cagatcgtcc ggttgcgcag gccgctcagc ggccgctcgt aaggaaagct
       61 gtacgcttcg aggaccgtgt agccgaagcg ctgcgcgacg ccgcgcaggt actcgggcga
      121 gaacagccag aagcccggca ccttgccgtg cggtgtcacc ccggcgcccg gctcggccgg
      181 cgcgtcgttc gacagcacca gccagctccc cggcggcggc ttgagcgcga gcagggtctc
      241 gaagaagccg gtgagcccgg tctccggatg gtacaggtgg ccgatcaggc cgtcggcgta
      301 gaccacgccg aagcgggcga ggtggcccgg cggtagcgtg tggtagtcgg cggtgaacgc
      361 gtcgacgccc ttcgctgccg ccagcgcgac ggcctcctcg atgcagtcga tggcctggac
      421 cggcagcccg cgggcctgca ggtcggcctc cacgaaggcg ttgccgcagc cgatcgagaa
      481 cacgcgctcg tgcggccgcg gcagcgacag gatcttgagc gccatgtgca tccggtattc
      541 cgggcagtag ctcgacggcg tcaccgaatc gccgaccgcc tcgccgcgct cccagacctc
      601 gtagcggttg agcgccgtgt cgccgaccgg acggcagtag aagtcgcgca tcgtgtccct
      661 cgccgggttc gtggcgggcg cggtcggctg cgacgcttcc cgcgcccgtg cggtgatccg
      721 cgactcctgg aggtactcga tggtcttggg cggttcctcg cgcagcgcca cgagcaacgg
      781 gtgaagcgcg tggctgccgg ccggcccgta ggcgcgatag aacgcgagcg cgtcgtggat
      841 ctgtcctgcg agcagcgtgt cggcgagcac ccgcgccatc gagcggaagc cgaggatcac
      901 ggccgcggga tcgccggtga gccgcggcat gcctccgtcg agcacgagcg cgccggacga
      961 cgccaggtag ttgagctgca ggtacgcccc atcggagtcg ggaaagttgc cgagcccgcg
     1021 atcgacgtag cgcagacact cggcgaggtg atacgcgacc acgtcggacg gctcgaggcc
     1081 gagccgcggc gccagcagct cggcgagcac gagcgtgccg aacacgcccg cggcggtctc
     1141 ctgcaggacg tacgacgccc agcgatcggc ggtgtcgagc gccgcgaacg cgccggggcg
     1201 gagcaccgag cggccgaatt gatgtgccag cacgccgagc acggggatcg cgccgaagct
     1261 cgcggcgctc gccggcagcc gcaggccgag gttgagcaag cggcgcccga gcggcagcga
     1321 caccgcatcg atcagcgcgc ggtgggtgtt ggcgaagtag tacgtcttca ggaatggaca
     1381 gcgttccatc ccttcgtcct cggggatgaa atacgcgatg tgcttggggt ggcggtcgcc
     1441 ctcgccggcc atgaagaaca accgcgtcgc ggtgaacgcg gggatctcac cgagccgcag
     1501 gtactcgtgc agcccgagca ggtacgcggt gacgtcgccg agccgtgcac gtgccgcggt
     1561 gaccgatgcc tgggacgcgg tgtcccggac gcagccgaag gcggtcggca atccgccggc
     1621 cggcttgccg taccacgtca ccggcttgcc ggcgacgatc gtgatgtcct cgtcgacggc
     1681 cgccagcgcc gccagcgtgt cgttgtcgtg gccgcgctcg accgcgtcgg ctctcagccc
     1741 gagcgcgcgc cgatcggcct cacccggtag cagctcggcg atccggcgca agcaacgggc
     1801 gatggtcgcc gcgtcctgca tgcgcgcggc ggggttgccg ccgagagcgt ggcagcgctt
     1861 ccactctgca tcgatcgcac cgcgcagcgc ctccagctcg gcgtgcagcc ccgcgccgag
     1921 cgcggggacc tcgttcgcca gatcgagata ggacaggcgg tagtcgtacg ccggtggcca
     1981 tgacacatgg ttccgctcgc ggtagctggt cgcacgttcg agattggtgc catacgcaaa
     2041 gcggtcgagt ttcacgctgt tccccccggt gttaatgcgc cgtggtgc
//
LOCUS       DASVOR010000348         2744 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_149279,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000348 DASVOR010000000
VERSION     DASVOR010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149279"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2291)
                     /gene="ppk1"
                     /locus_tag="VF516_07165"
     CDS             complement(<1..2291)
                     /gene="ppk1"
                     /locus_tag="VF516_07165"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEX8107491.1"
                     /translation="MPDRSVLASDMSGAHRLEVVTEALDLREPRDGREPREPDARDAR
                     AGLRSVDAPGRTPPAPALGDTVPTDLGTPELYLNRELTYLNFCWRVLHEAEDERVPLL
                     ERVKFVGIVSSNIDEFFQKRIGGLKQQVGAQLHRVTPDGRTPQQQISDCLELVTRLEA
                     RKQVILEKLIGELRAAGVWLAPFKDLDAQQQAWVREYYLTNIFPLVTPQAMDPAHPFP
                     FVSNLSLNLLVSLRYENDSEPLLARVKVPSSGGTPRFVRIGTSRTFVDLADVMANNLD
                     LLFPGMEIEACSKFRVTRNANTERDEEEAEDLLEMIEIELRERKFAPVVRLQVDKTMQ
                     PTLRGRLAAELGLDEQADVFEADGMLGQRDLMEIASLDAPELRDPPHAPVPPIDLLTD
                     GNIFHAIRDAKHLMVHHPFESFQHSVERFLREASDDPKVRAIKMTLYRTSKDSDVIKH
                     LVRAARNGKQVAVVLELKARFDEEANIKWASRMERAGIHVTYGVVGLKTHTKITLVVR
                     QDYDGLRRYAHLATGNYHAGTARLYTDLALFTCDDSIGRDLTELFNYLTTGYKPRRKY
                     QKLLVAPKQLKQALLEKIEREIALQGSGGRGHIQWKLNALEDVDIVRALYRASQRGVT
                     IDLIVRDSCRLRPGLDGVSSTIKVVSILGRFLEHERIYYFHNGGRPEYYIGSADAMQR
                     NLEKRVEVLVPIEDPRLQAELRHILDTQLTDQRGAWDMQPDGSYVQRAGMGAKHSQQQ
                     AIERTERRLKEATRLRRRKVQSTA"
     gene            complement(2358..>2744)
                     /locus_tag="VF516_07170"
     CDS             complement(2358..>2744)
                     /locus_tag="VF516_07170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107492.1"
                     /translation="VIRDGHAELFALSMHDAPKLGGMRLAPDHGPIAEPTAGPVTLTD
                     HVRSYEVVSGSDWHQLVAIDLTSGHALWARELGPAPVRAGGVAGGVAAGVVWIDQGAG
                     PHAFAALTGKDGGAPGPALDETRNHL"
BASE COUNT          368 a          926 c          988 g          462 t
ORIGIN      
        1 gcggtcgact ggaccttgcg gcggcgcagc cgggtcgctt ccttgagccg gcgctcggtg
       61 cgctcgatcg cctgctgctg gctgtgcttg gcgcccatgc cggcgcgctg gacgtagctg
      121 ccgtcgggct gcatgtccca cgcgccgcgc tggtcggtga gctgggtgtc gaggatgtgg
      181 cgcagctcgg cctgcaggcg cgggtcctcg atcggcacca ggacctcgac ccgcttctcg
      241 aggttgcgct gcatggcgtc ggccgagccg atgtagtact cgggccggcc gccgttgtgg
      301 aagtagtaga tccgctcgtg ctcgaggaac cggccgagga tgctgacgac cttgatcgtg
      361 ctcgacaccc cgtcgagccc gggccgcaac cggcagctgt cgcggacgat caggtcgatc
      421 gtcacgccgc gctgcgacgc gcggtacagc gcgcggacga tgtcgacgtc ctcgagcgcg
      481 ttgagcttcc actggatgtg gccgcggccg cccgagccct gcagcgcgat ctcgcgctcg
      541 atcttctcga gcagcgcctg cttgagctgc ttgggcgcga ccagcagctt ctggtacttg
      601 cggcgcggct tgtagccggt ggtcaggtag ttgaacagct cggtcaggtc gcggccgatc
      661 gagtcgtcgc aggtgaacag cgcgaggtcg gtgtacagcc gcgccgtgcc ggcgtggtag
      721 ttgccggtcg cgaggtgggc gtagcggcgc agcccgtcgt agtcctggcg cacgaccagc
      781 gtgatcttgg tgtgggtctt gagcccgacc acgccgtagg tgacgtggat gccggcgcgc
      841 tccatccggc tcgcccactt gatgttggcc tcctcgtcga accgcgcctt gagctcgagc
      901 acgacggcga cctgcttgcc gttgcgcgcg gcgcgcacca ggtgcttgat gacgtcgctg
      961 tccttcgacg tccggtacag cgtcatcttg atcgcgcgga ccttggggtc gtcggacgcc
     1021 tcgcgcagga accgctcgac gctgtgctgg aacgactcga acgggtggtg caccatgagg
     1081 tgcttggcgt cgcggatcgc gtggaagatg ttgccgtcgg tgagcaggtc gatcggcggc
     1141 accggcgcgt gcggtggatc gcgcagctcg ggggcgtcga gcgaggcgat ctccatcaga
     1201 tcgcgctggc cgagcatgcc gtcggcctcg aacacgtcgg cctgctcgtc gaggccgagc
     1261 tcggcggcga gccggccgcg cagggtcggc tgcatggtct tgtcgacctg gagccggacc
     1321 accggggcga acttgcgctc gcgcagctcg atctcgatca tctcgagcag gtcctcggcc
     1381 tcctcctcgt cgcgctcggt gttcgcgttg cgggtgaccc ggaacttcga gcacgcctcg
     1441 atctccatcc cggggaacag gaggtcgagg ttgttggcca tcacgtcggc gaggtcgacg
     1501 aacgtgcgcg acgtgccgat ccggacgaac cgcggggtgc cgcccgacga cggcaccttg
     1561 acccgggcga gcagcggctc gctgtcgttc tcgtagcgca gcgagaccag gaggttcagc
     1621 gacaggttgg agacgaacgg gaacgggtgc gccgggtcca tcgcctgcgg cgtgaccagc
     1681 gggaagatgt tggtcaggta atactcgcgg acccaggcct gctgctgggc gtcgaggtcc
     1741 ttgaacggcg ccagccacac cccggccgcg cgcagctcgc cgatcagctt ctcgaggatg
     1801 acctgcttgc gggcctcgag ccgcgtgacc agctccaggc aatcgctgat ctgctgctgc
     1861 ggcgtgcggc cgtccggtgt cacccggtgg agctgggcgc cgacctgctg cttgaggccg
     1921 ccgatccgct tctggaagaa ctcgtcgatg ttcgagctga cgatgccgac gaacttgacc
     1981 cgctccagga gcggcacccg ctcgtcctcg gcctcgtgca gcacccgcca gcagaagttg
     2041 aggtaggtca gctcgcggtt gaggtagagc tccggggtgc cgaggtcggt cggcacggtg
     2101 tccccgagcg cgggcgccgg gggggtccgc cccggcgcgt cgaccgaccg gagccccgcc
     2161 cgcgcgtcgc gcgcgtcggg ctcccgtggc tcgcggccgt cccggggctc ccgcagatcg
     2221 agcgcctccg tcacgacctc caggcggtgt gcccccgaca tgtcgctcgc gagaaccgat
     2281 cgatcgggca tcaccggaaa agtatacctc ctcggtgacg agtctgtcgc tggagttgac
     2341 ccgggattcc cttgttatta caaatgattg cgcgtttcat ccagggcggg gcccggggca
     2401 cctccgtcct tgccggtgag cgccgcgaac gcgtgaggcc cggctccctg atcgatccag
     2461 accactccgg cggccactcc gccggccaca ccgccggcgc ggaccggcgc aggaccgagc
     2521 tcccgcgccc acagggcatg gcccgaggtc aggtcgatgg cgaccagctg gtgccagtcc
     2581 gaccccgaca cgacctcgta cgaccgcacg tggtcggtca gcgtgaccgg cccggcggtc
     2641 ggctcggcga tcgggccgtg gtccggtgcc agccgcatgc cgcccagctt gggggcgtcg
     2701 tgcatcgaca gcgcgaacag ctcggcgtgg ccatcgcgga tcac
//
LOCUS       DASVOR010000349        18931 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266061,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000349 DASVOR010000000
VERSION     DASVOR010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18931
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266061"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VF516_07175"
     CDS             <1..673
                     /locus_tag="VF516_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HEX8107493.1"
                     /translation="EHRIRLPREVVAAVRAQVAGRGVVGCRYLGDDVIDGGSHLPDAT
                     WFGVELARAGLDFLSISKGGKFEDAKQPKVGWAAYPYTGRSGYECMPTIYSDAAGPFR
                     RNVPLAAAIRAAVREAGFATPIVAAGGICEFAQAEAILAAGEADLIASARQSLADPDW
                     FLKIREGRGGEIRRCEFTNYCEALDQQHKQVTCKLWDRDALDAPDVTLAADGKRRLIA
                     PRWQR"
     gene            955..4470
                     /locus_tag="VF516_07180"
     CDS             955..4470
                     /locus_tag="VF516_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8107494.1"
                     /translation="MTDLDLSGRKLGEFVLRERIGAGGYGTVYRAEQPALQREAVVKV
                     LRPRWRNDAAPERFLREAQLASRLDHPYAAHVYSFGVEDDDDALRWIAMELVHGITLA
                     EWLRSRGSMPFARFVPFFECVAQVVQAAHARGIVHRDLKPSNIMVLESGGQLFPKLLD
                     FGVAKLLEDPDQPGEDPEDDGARSPADAVATVRNRAELSPERTITGPDCGPGRLTPSH
                     GAVIGSVPYMSPEQWDDPRNVGPATDLYSLGCVAYEALTGHPPFMPETSGGYYELHRR
                     AEPPPLGDGFPPGVDQALRRALAKSSDARHANALELAAELRAVLRAEPREQLRVSAQQ
                     WHDRGRPAAMLWHDDVLAALEQWMQRSARSGALTVLELEFVEASKGLAAAQSEVRRRR
                     NARLHLATAALAFAGAAAGLGALQWRTVERARNSQQVSEAQRAAAEVTAELEQGRAAL
                     LHGEMDEAQRHLGEAWRRGERSSSTAFMLARAMQPFRAELARFKSTSGPMWSATFSPD
                     GRQTATTDDRSAQIWNAENYQQLFPLPHEDIVYHAVYTSDGARLITAAGDGAVRIWNA
                     ATGSLVRELRLHRHSPRYYRAAVSPDSKLVAAIDTLGRSAVVWNTATGAVLAELANDG
                     SERPSIAFSPDGRWLATGGGSYVCVFDTRRWRQVLAIPGPKIQAISWDPTGPRLLTGS
                     VAGDASIWAIPGGARLHHMREIGEPVDAVVFSPDGRLVAAASRDGLEEVFDPSSDRMV
                     SRANYLHSKILSIEFDPASRLVVAAGERGTTVVGDAMQGISVAALEGPKQRIRAVHFE
                     PGARRVIGASWEGTAYVWDVSAPYRRWSSLAIADSCDLFGGVVPDGRFLAIGCVGHPT
                     RVWDTARDQLLAELPAVVAPSNDFAAAFPAVSVAGDLAAVARGNAVELYGLPGGRPAR
                     TIDHGAPVTAVAFGEGHDVVSGAADGSIRATHDAGDPVPLPPASAGIDAVACLRDGRV
                     VVADAAGHIRIVDRGVVVAELHAPARVRSLRLSPDGARLVAIASYTGNVAPPTLWALD
                     RPRLVAELDGHAGQVLSARFVEDGRGILTAANDGVVRLWDAATGRLRQTFQGTSRFLA
                     DATLDTAGKMLVAGDGDGSVRWWDVATGRPLWTLQVHKSIVVGLHFEGDDLLTRGFAG
                     DVSRWTIPPAKNVIDRCDACGIVAP"
     gene            4892..5950
                     /locus_tag="VF516_07185"
     CDS             4892..5950
                     /locus_tag="VF516_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8107495.1"
                     /translation="MAAITSVEIENFRCFQKLRVDGLAQVNLIVGANNAGKTVLLEAI
                     EAVVSRDSPFVLYRASLERNEYRRHVGPDEDLVEIDLRHWFHGHALTDGASFSIRAAG
                     EQQFAMSRSLEIVPSDAPSHPFAPEGLWLTTRRPGARAMPSLPLTADGLLGAGPLSRF
                     NNFGTRLTPPVGFVSTNRLSPRQLARLWTNVVLTPGEQRTIDALRLIEPKIERIAISE
                     ADSTIAKVLLRGADAPVPLGTLGEGVSRILTLALQLALTPRGFLMIDEIENGLHWSVM
                     PKVWRFLIETALAGQTQVFAATHSKDWLEGLADLHRSNPQLAAHVAVHRLEAGRPTSV
                     RFTAERIAEYVEMEMEAR"
     gene            6185..6598
                     /locus_tag="VF516_07190"
     CDS             6185..6598
                     /locus_tag="VF516_07190"
                     /inference="COORDINATES: protein motif:HMM:NF022971.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3226 domain-containing protein"
                     /protein_id="HEX8107496.1"
                     /translation="MLKPTTSGLPYPRSCRSSAWIRVAGPDSAGALVPGRYGIWMWPD
                     NVSHGDLESFVAGILPQTPIFAYAAEACRIAKDDHAAEYEPRHARKAALKVRSVWRDA
                     SAAGGYGHLVRNLPLTQTPACDAFMVWFRTLFLTD"
     gene            complement(6618..7085)
                     /locus_tag="VF516_07195"
     CDS             complement(6618..7085)
                     /locus_tag="VF516_07195"
                     /inference="COORDINATES: protein motif:HMM:NF013401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEX8107497.1"
                     /translation="MHPPFEPSCGVCQSLSGDRREPALYQDDLWVVRPASPPGVPGWM
                     MMITRRHVGGPAHFDDREARAFGPALRHFERILERVTGALRIYTAALGESHPHFHAHM
                     VPRYAAMPRDARAWSVFDLQRAVAAGEVAIDANAHAEICAAYQRALAGEPPPT"
     gene            7201..7557
                     /locus_tag="VF516_07200"
     CDS             7201..7557
                     /locus_tag="VF516_07200"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8107498.1"
                     /translation="MTKFRRTLGATSLIQDKVVNLAGQDIGRIEELMIDVTTGRVAYA
                     VMSFGGFMGIGNKLFALPWSAVTVDEAKKRFVVNVTRESLDQMPGFDKDHWPDLGDLE
                     YAIGVYRQWGATPYWT"
     gene            complement(7714..7971)
                     /locus_tag="VF516_07205"
     CDS             complement(7714..7971)
                     /locus_tag="VF516_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107499.1"
                     /translation="MKTCECCGNSYDKVFELRIGGTTHIFDSFQCAIHVLAPRCFHCK
                     VPIIGHGLEARGQYFCCAHCARQEGIEDVKDRAPDKGVAPP"
     gene            8053..9162
                     /locus_tag="VF516_07210"
     CDS             8053..9162
                     /locus_tag="VF516_07210"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8107500.1"
                     /translation="MSRSTSRIRFAVIGLGHFAQSAVLPAIEQLLDVELTALVSGSRH
                     KLDELGRRYGVRALCSYDRLDQLLASDAVDAVYVAVPNDLHAEMTLVAARHGVHVLCE
                     KPMAPTEAECMQMIRACEQRRVKLMIAYRLHFEAANLVAIELARGGDIGDPRLFSSVF
                     SMQVRSGNVRTQPRRGAGPLYDIGIYCLNAARYLFRTEPVEVSAMRFAGRDPRFAAVD
                     EAYAATLRFPVDRVAQFTASFGAHDRAHYQVIGTEGFLTLDNAYEYAAEGMTLQVVGK
                     HGAKTRTFPRRDQIAAEIEYFARCIRDDHDPEPSGWEGLADVRILQALQSSARFGRAV
                     PIDPVPRPRRPDLGQEIRIPAHEMPPLVDVEQPAK"
     gene            complement(9175..10062)
                     /locus_tag="VF516_07215"
     CDS             complement(9175..10062)
                     /locus_tag="VF516_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107501.1"
                     /translation="MRTPLCLFALAALASGCHGDKPAPPAQGSGSTAASIIPGSGSGP
                     GSSVAATVVPGGVELFVNDLTIGSVQPSLIANWPRLDALVPGNARKLGTWDAVTLQGG
                     KPTPTELTHPSTNFPDMVPAVFPGDGGAPAFGMFDPVELARKGKPAMREDHVTAIRIK
                     LAQGGNRGQNDDSGGTTADPTQLVIAVKTAGGMSSLTGPQVLALPREGTPGSPDQKGW
                     RLAALLEATGVKTFEHLALRDASGNSLTLDKADVSDTSVPFVKLNKQGMLRLKVYKKT
                     GDGWNAAGDLRGLTAIEVK"
     gene            10102..11574
                     /locus_tag="VF516_07220"
     CDS             10102..11574
                     /locus_tag="VF516_07220"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8107502.1"
                     /translation="MEKLAQEIVPILVLVAVIAVVVARLPRVDVSHTPAFRRRRTLNW
                     VPLGLTYAFLYMGRYNLAVLKNVGGISQHDYGNIDGWGSVVYGVSFLVNGPLADRLGG
                     RRTILIAAGGSLAANALIGVLWKTGAIDKDAITALTVLFVLNMYFQSFGAVSIVKVNA
                     SWFHLRERGTFGGIFGILISLGYYFSLDWGRRIADAACPACGPYANGEPDAPAPTLAG
                     SLGLAWLFLVPAAILAVFWALSFLWVRDKPSDAGLSDFDVADASSGQRETREPAAQVI
                     WRMLKHPVILTIAIIELCSGFLRQAITKWGPDFARGVGAGQSYVNQHWGMVTCIAGIT
                     GGMFAGVISDHLFQSRRPPMSTVLYLIMLVGALAILPLLGAPVVVGWIVAFMAMAIIG
                     VHGMLSGVASQDFGGRLNTGVAVGLIDGFVYLGSAIQDQVYGGALSLLPEKGDKAARL
                     IESWRAWPVAMVPAAAIGLALSIRIWNARVVRPAAAAASR"
     gene            11584..12414
                     /locus_tag="VF516_07225"
     CDS             11584..12414
                     /locus_tag="VF516_07225"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX8107503.1"
                     /translation="MARAKSLSRAGVERERGCEREKIDAVGTLLTQVSSALFITGPGL
                     SIESGLPHYRGIPGLVRRTAQDARMIESALSIDTLHKKPKLTWRTLLEIDRRVCEAEP
                     GRGHEVLVALERRLERATIMTVNVDRLHQRAGSRSVIEMHGALHDLRCARCEISTRHD
                     RYAGLPLPPLCAACGSVLRPDMALFGEPLPPDPFTRLQAELEIGFDIVFAIGVVAVFP
                     YLARPVLLAKQEGIPTVEIAHAATEISEIVDFGLRGSPGRILELIWEVFAQLAGRRPG
                     "
     gene            12653..14689
                     /locus_tag="VF516_07230"
     CDS             12653..14689
                     /locus_tag="VF516_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107504.1"
                     /translation="MALQNKLTTAWILSFLIGCGTSPATGSGDGQDPMGTEGGSPDGS
                     GTPGGGMTTGSTTPVFPTAHPRIYLTPNRARLAAALSAGTPAATRFKAIVDQWVGGAD
                     LWGFSAWNAALIGQLTGNATYCTKAVAAIETQVSTAEGKIAAGQAPEVAADSYLGVGD
                     MIGDLALVYDWCFDKVTAAQRTRWLAYADQAVWNVWHASAAKWGSATIPWTSWSTDNP
                     MNNYYYSFMRATMLLGLAAKGEDSQADAWITEFHDTKLMNQLMPRFNADLAGGGSREG
                     TGYGVAMRRLFELYDLWQSTTGENIGAATPHTRQSMLSAVHQLVPTRDRFAPTGDQSR
                     DSTASFFDYQRNYLQELIQAFPGDKLSARAKTQLQGSNLPTMSMGFMAVYDFLYDDAA
                     VTAAPVDLNTTYYASGIGELYARSGWDTHATWVNLIAGPYTEDHAHQDQGSLMIYKDG
                     WLAYDSVIDSHSGLPQDTTAHSLVRITSGGAPVRQVVGTKPQLSSLHQGTGYTYAAAD
                     LTAAYNGNSAVQKVQREVVYLQPDVVVVFDRVQSAAGTTQTWQLASPAAASISGNLAS
                     IASSGHTLKVQQIAGGSMSVTPFTSVSSDYAGGFRLDETMSGGDNRYLHVLSIDGAAS
                     SVTPAGDASNPGVTVAMSNGHTATVTFVRDNTGATLVLDGATKSLAVGLDRLPE"
     gene            14755..16710
                     /locus_tag="VF516_07235"
     CDS             14755..16710
                     /locus_tag="VF516_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107505.1"
                     /translation="MASACGVDGPEFSADHPRIYMKSRKDALVAALQAGQPEAKRFKD
                     MVDGWVGGGDVYNFFSWNAALLGQLTGDPKYCAAAIKDVEQQVNDATSQVNGGQLPFV
                     AHDSYLDVGNLIGNLALVYDWCAESLPADRRGAWLSYADQAVYNVWNPDVAKWGNLPD
                     ATWSGFAIDDPSDNYFYSFLRATMLLGLAAHDETPRAKGWLSEFRDTKLAGELVPVFD
                     MQLVGGGSREGTGSGVSQRDLFELYDLWKASTGEPIADLTPHTRASMLAMIHQIVPTL
                     DRIAPTGDQSRDSLAQFFDYHRSYLQDLIALYSTDPVAPHAQALLASSSLPQMSQQFM
                     YASDFLYANRNVTATALDGLGTAYFAPGIGEVYARSGWDKHATWVNLIAGPYSQSHAH
                     QDQGSLMIYKDGWLAYDVVIDSHSGVNPPVQATSAHSLVRIVDASGKTVAQQAKNLLN
                     PDDQSKPDSKVVALHRPKGAGFLHVAADLTAAYGGQSTVQQVQREIVYLEPSTVIVYD
                     RLATKVGTQVVFQLVSPVSPPTATGTTHTFTTAGHTLTLEDVGPVLGMPAIFDFAKSD
                     SSEFMGGFRLDEPAPGGNNVRFLHVLTIDGAATGIVQGDTPGDSVKLTVGGQDVVMTF
                     NHDAIGGTLTIGTQSFPLGASLDPLPE"
     gene            complement(16834..>18931)
                     /locus_tag="VF516_07240"
     CDS             complement(16834..>18931)
                     /locus_tag="VF516_07240"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8107506.1"
                     /translation="VAHMGSLTASEFSMQDKRLLSAMANRATSAIFQHLLREAAERAS
                     AELSAILESMPAAVFIGNERAVTRANRAGLDLLGYDDSDQLARQPVVSLVEALDMRDA
                     GSGARLDVGNGPVRTAVRGVTYQRDVLIHDHTAAKDAIVHAVAAPIVRDGNVIGAVAV
                     AVDVTAHKRIEQALRERELEFRTLAENIPQLVWIADTTGSAYWFNQRWFDYTGTKLAD
                     VEGWGWQRVQHPEHVERVTETIRRAIAGEEPWEDTFPIRGQDNIYRWFLSRAVPVRDE
                     SGQVIRWFSTATDVTDQRFLSNATKLLASSLDMMATLEQLANLAVPELADWCVVDVLK
                     DGQIERVAIAHSDRTKVTVVREWSRRYPSDLQASGGIGEVIRTGRAMIYPEFSDAQLV
                     HIARDAAHLRLMRELGISSVIVAPLVAHGETLGAVTLIRAESGRSYGPSKLETAMELG
                     RRAGYAIENARLYRDAQDATRAREEILAIVSHDLRNPLATIDLGATVALESSDIGPKA
                     SKHVEAIHRAASRMGHLLGDLLDAASLRAGRLPMAMKNEDVAALVTRVIEAHEELARK
                     QGIDLVCDCPVQDARICGDRERLMQAFGNLIGNALKFCGRGDVVTVRARVADRQVAFE
                     VADGGPGIADDELPHIFEPYWSAKQHAKKGIGLGLYICKAIIEAHSGTISVASKLGRG
                     TTFSMAIPLADPGSAS"
BASE COUNT         2864 a         6486 c         6628 g         2953 t
ORIGIN      
        1 cgagcaccgc atccggctgc cgcgcgaggt cgtcgccgcg gtgcgcgctc aggtcgccgg
       61 ccgcggcgtc gtcggctgcc gctacctcgg cgacgacgtc atcgacggcg gcagccacct
      121 gcccgacgcg acctggttcg gcgtcgagct ggcgcgcgcc ggcctcgact tcctgtcgat
      181 ctccaagggc ggcaagttcg aggacgccaa gcagcccaag gtcggctggg ccgcctatcc
      241 gtacaccggc cggtccggct acgagtgcat gcccaccatc tacagcgacg ccgccggccc
      301 gttccgccgc aacgtcccgc tcgccgccgc gatccgcgcc gcggtgcgcg aggccggctt
      361 cgccaccccg atcgtcgccg ccggcgggat ctgcgagttc gcgcaggccg aggcgatcct
      421 cgccgccggc gaggccgacc tcatcgcctc cgcccgccag agcctggccg accccgactg
      481 gttcctcaag atccgcgagg gccgcggcgg cgagatccgg cgctgcgagt tcaccaacta
      541 ctgcgaggcg ctcgaccagc agcacaagca ggtcacctgc aagctgtggg atcgcgacgc
      601 gctcgacgcc cccgacgtca cgctcgccgc cgacggcaag cgccgcctga tcgccccgcg
      661 ctggcagcgt tgagcggccg aggttggcgt cgatctccgg cgggagcacg ccgccctgcc
      721 tcgcggtttg agcttctcac cgatttcgca tccgacactc cgctcgagag cggcattcgc
      781 tcgaatgaag agctgatgct tccatcgtag cgcgcgcgga gctcgccaca gctcgggaga
      841 cgtgcgcggt ggtatcacct cggcgcacta gcactatcga agaaggcagc atattgctgc
      901 agtcggatgg agggacgccg aagggtacag atgcatagcc ttgcggctca gccgatgacg
      961 gatctcgatc tctcgggccg gaagctgggc gagttcgtgc tgcgcgagcg gatcggcgcc
     1021 ggcggatacg gcactgtgta ccgcgccgag cagccggcac tccagcgcga ggcagtcgtc
     1081 aaggtgctgc gtcctcgatg gcgcaacgat gccgcgccgg agcgcttcct gcgcgaggcg
     1141 cagctggcct cgcggctcga tcacccgtac gccgcgcacg tgtactcgtt cggtgtcgag
     1201 gacgatgacg acgcgttgcg atggatcgcc atggagctcg tacacggcat cacgctcgcc
     1261 gagtggctcc ggtcgcgcgg gtcgatgccc ttcgcgcggt tcgtcccgtt cttcgagtgc
     1321 gtcgcccagg tcgtccaggc cgcccatgcg cgcggcatcg tgcaccgtga cctgaagccg
     1381 tcgaacatca tggtgctcga gagcggaggg cagctgttcc ccaagttgct cgacttcggg
     1441 gtcgccaagc tgctcgaaga tccggatcag cccggtgagg atcccgaaga cgacggcgcg
     1501 cgcagccccg ccgacgccgt cgcgacggtg agaaaccggg ccgagttgtc tcccgagcgc
     1561 acgatcacgg ggccggactg cgggcctggc cgcctgacgc cctcccatgg cgcggtgatc
     1621 ggttcggtgc cgtacatgtc ccccgagcaa tgggacgacc cgcgcaacgt cggtcccgcg
     1681 accgacctct attccctcgg ctgcgtcgcg tacgaggcgc tcaccggaca cccgccgttc
     1741 atgccggaga cttccggggg ctattacgag ctccaccgcc gcgccgagcc tcccccgctc
     1801 ggcgacggct ttccgccggg cgtcgaccaa gcgctccgtc gcgccctggc taagtcctct
     1861 gacgcgcgcc acgccaatgc gctggagctc gccgcggagc tccgcgccgt gctgcgcgcg
     1921 gagccgcggg agcagctccg cgtctcggcg cagcagtggc acgaccgagg caggcccgcg
     1981 gcgatgctgt ggcacgacga cgtgctcgcc gcgctcgagc agtggatgca gcgctcggcg
     2041 cgctccggcg cgctcaccgt cctcgagctc gagttcgtcg aagcgagcaa agggctcgcg
     2101 gccgcacagt ccgaggtacg tcgccgccga aacgcgcggc ttcacctcgc cacagcggct
     2161 ctcgcctttg ccggtgccgc ggcggggctc ggggcgctgc agtggcgcac cgtggagagg
     2221 gcgcgcaact cgcaacaggt ctcggaggcg cagcgagcgg ccgccgaggt cactgccgag
     2281 ctggaacagg gccgcgcggc cctgctccat ggcgagatgg acgaggcgca gcggcacctt
     2341 ggcgaggcgt ggcggcgcgg cgagcgatcg tcgagcacag cattcatgct cgcacgcgca
     2401 atgcagccgt tccgcgccga gctcgcccgg ttcaagtcga cgtcaggccc gatgtggtct
     2461 gcaacattct cgccggacgg ccggcagacc gccacgaccg acgacaggag cgcgcagatc
     2521 tggaacgcgg agaactacca gcagttgttc ccgcttcccc acgaggacat cgtctatcac
     2581 gccgtgtaca cgagtgatgg cgcacggctc atcaccgccg ccggcgatgg cgcggtgcgg
     2641 atctggaacg cggcgaccgg atcgctcgtg cgcgagctgc ggcttcaccg tcattcgccg
     2701 cgctactacc gtgcagcggt gtcgccggac agcaagctcg tcgccgcgat cgacacgctc
     2761 ggcaggagcg ccgtggtctg gaatacggcg accggcgcgg tgctcgccga gctggccaac
     2821 gacggatcgg agcggccgtc gatcgcgttc agccccgacg gccgctggct cgcgaccggc
     2881 ggcggcagct atgtttgcgt gttcgatacg cggcgctggc gccaggtact cgcgatccca
     2941 ggacccaaga tccaggcgat cagctgggat ccgaccgggc cgcgtctcct cactggcagt
     3001 gtcgccggcg acgcctcgat ctgggcaatt cccggcggcg cgcggttgca ccacatgcgc
     3061 gagatcggcg agcccgtgga cgccgtggta ttttcgccag acggccggct cgtggcagcg
     3121 gcgagccgcg atggcctcga ggaggtgttc gacccgagct cggatcgcat ggtgagccgg
     3181 gccaattacc tccacagcaa gatcctgtcg atcgagttcg atcccgcgtc gcggctcgtg
     3241 gtggcggccg gtgaacgtgg aacgaccgtc gtcggcgatg cgatgcaggg gatctcggtc
     3301 gccgcgctgg aaggacccaa gcaacgtatc cgggcagttc actttgaacc tggcgcacgt
     3361 cgggtcatcg gggcctcatg ggagggcacg gcatacgtct gggacgtgag cgcgccgtac
     3421 cgccgctggt cctcgctggc gatcgccgac agctgcgatc tgttcggcgg cgttgtgccc
     3481 gatggtcggt tcctggcgat cggctgcgtc ggccacccca cgcgggtatg ggacaccgcg
     3541 cgcgaccagc tgctggccga gctgccggcg gtcgtcgcgc cgagcaacga cttcgcggct
     3601 gcctttcccg cggtgtccgt cgcaggcgac cttgccgcgg tcgcacgcgg caacgcggtg
     3661 gagctctacg ggctcccggg cggtcggccg gcgcgtacga tcgaccacgg cgctcccgtc
     3721 accgcggtcg cgttcggcga ggggcatgac gtggtcagcg gcgccgccga tggatcgatc
     3781 cgcgcgaccc acgacgccgg agatccagtc ccgctgccgc cggcgtccgc cgggatcgat
     3841 gcggtcgcgt gccttcgtga tggtcgcgtc gtcgtggccg acgcggctgg tcacatccgc
     3901 atcgtcgatc gcggcgtcgt cgtcgcagag ctccacgccc cggcgcgcgt ccgctcgctc
     3961 cggttgtcac cggacggcgc gcggctggtc gcgatcgcga gctacaccgg gaatgtcgca
     4021 ccaccaacgc tatgggcgct ggaccggccg cgtctcgtgg cggagctcga tggccacgcc
     4081 gggcaagtgc tgtccgctcg cttcgtcgag gacggtcgcg ggatcctcac tgcagccaac
     4141 gacggggtcg tgcggctctg ggacgcagcc acggggcggc tgcggcagac ctttcagggc
     4201 acgtcgcgat ttctcgccga cgctacgctg gataccgcgg ggaagatgct ggtggccggc
     4261 gacggtgacg ggtcggtgcg gtggtgggac gtggcgaccg gacgcccttt gtggacgctt
     4321 caggtgcaca agtccatcgt ggtagggctt cacttcgagg gcgacgacct cctcacccgt
     4381 ggcttcgcgg gtgacgtctc acggtggacg atcccacccg cgaagaacgt gatcgaccgg
     4441 tgcgacgcct gcggtatcgt ggctccgtga agaagcccac ccgtacccta cagcttcgcc
     4501 gcgaaacgct ccgggccctc gcggtgctgg aactcgcgca cgcagtcggc ggccaacccc
     4561 cgctgcttct tgactcgggc gtggcgcaga cctgcccgaa cgtaaacgct tcagcgctcc
     4621 cgccgcctcc gcgcggctga tccgaccgcc gcgccgtctg gccgagggac cgcagcagac
     4681 ctgcctcgaa gggaagctag tgcaaggcgc tcgaccagca gcacgagcag gtcacctgca
     4741 agctgtggga ccgcgacacg ctcgatgtcc ccgatgtcac gctcgccgcc gacggcaagc
     4801 gccgcccgat cgcccgcgct ggcggctgag cgttccgcca ctgggccctg gcgcgcggtg
     4861 cacaaccggc gggttcccag taggatgcgg tatggccgcg atcacatcgg tcgagatcga
     4921 gaacttccgc tgcttccaga agctgcgcgt cgacggcctc gcgcaggtca acctgatcgt
     4981 cggggccaac aacgccggca agacagtgct gctggaagcg atcgaggcgg tggtgtcacg
     5041 cgacagcccg ttcgtgctct accgtgcgtc gttggagcga aacgagtatc gccggcacgt
     5101 ggggccagat gaagatctcg tggagattga tctgcggcac tggtttcacg gccacgccct
     5161 caccgacggc gcaagcttca gcattcgtgc ggctggggag cagcagttcg cgatgtctcg
     5221 gagtctggag atcgtaccgt cagacgctcc gagtcatccg tttgctccag aaggattgtg
     5281 gctgacaact cgacgtccag gcgcgcgtgc tatgccctcg ctaccactca ccgcagatgg
     5341 cctcctcggt gcggggccgc tgtccaggtt caacaacttc ggcacgcgat tgactccgcc
     5401 ggtcgggttc gtatcgacga atcggctgtc tcccaggcag cttgcacggc tctggacaaa
     5461 tgtcgtcctg acccctgggg aacaacgtac gatcgacgca cttcgcctca tcgaacccaa
     5521 gatcgagagg atcgcgatct ccgaggccga tagcacgatc gcgaaggtgc tgttgcgtgg
     5581 agcggatgct ccggttcccc tcggcacgct cggcgagggc gtgtcgcgga tcctcacact
     5641 cgcgctccag ctcgcgctca cgccacgtgg gttcctgatg atcgacgaga tcgaaaacgg
     5701 cctccattgg tccgtcatgc ccaaggtttg gcgcttcctg atcgagaccg cgcttgccgg
     5761 gcagacgcag gtcttcgcgg ccacgcactc gaaggactgg ctggaggggc tggccgatct
     5821 gcatcggagc aacccgcaac tcgcggcgca tgtcgctgtt caccggctgg aagcggggcg
     5881 gccgacctcg gtccggttca ctgccgagcg gatcgccgag tacgtcgaga tggagatgga
     5941 ggcccgctga tggtgcggga gatcaacacc aagatcgacg tgctgttcgt cgagggcccg
     6001 gatgacggtg cgctggtgaa tgctctcgtg aaccggctca tcggcatcga cctggctcgg
     6061 gagccgcata aagtcgtgaa ggctcggccc gacggcggcg gtgacacctg ggcgctccgt
     6121 cagttcaaga agcgcatcga ggaaggccgc acggaggcgc gtatcggctt gatcgtcgac
     6181 cgcgatgctg aaaccaacga caagtggcct gccgtatccg cgatcttgca ggagctcggc
     6241 ctggatacga gtagcgggcc ccgacagcgc cggagcgctc gtcccgggtc ggtatggcat
     6301 ctggatgtgg cccgacaacg tcagccacgg cgatctggag agcttcgtcg ccggtatcct
     6361 cccgcagacc ccgatcttcg cttacgccgc cgaggcttgc cggatcgcca aggacgacca
     6421 tgcggccgag tacgagcctc gccacgcgcg caaggccgcg ctgaaagtcc gcagcgtgtg
     6481 gcgcgacgcc agcgcagccg gtggctacgg ccacctcgtt cgcaacctac cgctcacgca
     6541 aacgcccgcg tgcgatgcgt tcatggtgtg gttccgcacg ctgtttctga ccgactgatt
     6601 gcgcaggcga gggtcgatca ggtcggcggg ggctcgccgg ccagggcgcg ctggtacgcg
     6661 gcgcagatct cggcgtgggc gttcgcgtcg atggcgacct cgccggcggc gaccgcgcgc
     6721 tggaggtcga acacgctcca cgcccgggcg tcgcgcggca tcgcggcgta gcgcgggacc
     6781 atgtgggcgt ggaagtgcgg gtggctctcg ccgagcgcgg cggtgtagat ccgcagggcg
     6841 ccggtcacgc gctcgaggat gcgctcgaag tggcgcagcg cggggccgaa ggcgcgggcc
     6901 tcgcggtcgt cgaagtgcgc cgggccgccg acgtggcgcc gggtgatcat catcatccac
     6961 ccggggacgc cgggcggcga ggcgggacgg acgacccaga ggtcgtcctg gtacagcgcg
     7021 ggctctcggc ggtcgccgga cagcgactgg cagacgccgc agctgggctc gaagggcgga
     7081 tgcacgaggc gagcatggcg gcgccgcgcg atgcgcaggt gacgcgcgcg tggctggtcc
     7141 gcaacacgac ggcggccgct ggaacgcggc ttgctgcgct ggggaagaag gagaccagcc
     7201 atgacgaaat ttcggagaac gctcggggcg acgtccctga tccaggacaa ggtcgtcaac
     7261 cttgccggcc aggacatcgg ccggatcgag gagctgatga tcgacgtgac gaccggacgc
     7321 gtggcctatg cggtgatgtc gttcggcggc ttcatgggga tcggcaacaa gctgttcgcg
     7381 ttgccgtggt ctgcggtcac ggtggacgag gcgaagaagc gcttcgtcgt caacgtcacc
     7441 cgagagtcgc tggaccagat gccggggttc gacaaggacc actggccgga tctcggcgat
     7501 ctggagtacg cgatcggcgt gtaccgccag tggggcgcca cgccgtactg gacataggtg
     7561 ggtggcgggc accgccgcac gtgcgcgctg cggcactgtg cgcggcggcg cgatcgaagc
     7621 gccgtcgggc gccgtcggag cgcggccggc gcgggaagga cggtcggcag ggggcgcggt
     7681 gcgctcgcga tccgcgcaac ggtcgccggg cgctcagggc ggcgcgacgc ccttgtcggg
     7741 cgcacggtcc ttgacgtcct cgatgccctc ctggcgcgcg cagtgcgcgc agcagaagta
     7801 ctggccgcgc gcctccagcc cgtggccgat gatcggcacc ttgcagtgga agcagcgcgg
     7861 cgcgaggacg tgaatcgcac actggaatga atcgaagatg tgcgtcgtgc ccccgatcct
     7921 gagctcgaac accttgtcgt aggagttgcc gcaacactca caggtcttca tggccattgt
     7981 tgtctcgtgt gcaagcctcg cgccgagggg gcgctcgcgc ggctggaatg cggcttgctg
     8041 ccgatggaga gcatgagtcg ctcgacatcg cggatccgtt ttgccgtgat cgggctgggg
     8101 cacttcgcgc agtccgcggt gcttccagcg atcgaacagc tgctcgacgt cgagctgacc
     8161 gcgctggtgt cgggatcgcg gcacaagctc gacgagctcg gccggcgcta cggcgtgcgc
     8221 gcgctgtgca gctacgaccg gctcgaccag ctgctcgcca gcgacgcggt cgacgcggtg
     8281 tacgtcgcgg tgcccaacga cctgcacgcg gagatgacgc tggtcgcggc gcgccacggg
     8341 gtgcacgtgc tgtgcgagaa accgatggcg ccgaccgagg ccgagtgcat gcagatgatc
     8401 cgcgcgtgcg agcagcgccg ggtcaagctg atgatcgcgt accggctgca cttcgaggcc
     8461 gcgaacctgg tggcgatcga gctcgcgcgc ggcggcgaca tcggcgatcc gcggctgttc
     8521 agctcggtgt tctcgatgca ggtccgcagc ggcaacgtgc gcacccagcc gcggcgcggc
     8581 gccggaccgc tctacgacat cgggatctac tgcctcaacg ccgcgcgcta cctgttccgc
     8641 accgagccgg tcgaggtgtc ggcgatgcgg ttcgccggcc gcgacccgcg gttcgcggcg
     8701 gtcgacgagg cgtacgccgc gacgctgcgg ttcccggtcg accgggtcgc gcagttcacc
     8761 gccagcttcg gtgcccacga tcgcgcgcac taccaggtga tcgggaccga ggggttcctg
     8821 accctggaca acgcgtacga gtacgccgcc gaggggatga cgctgcaggt ggtgggcaag
     8881 cacggcgcca agacccggac gttcccgcgg cgcgatcaga tcgccgccga gatcgagtac
     8941 ttcgcgcgct gcatccgcga cgaccacgac cccgagccga gcggctggga gggcctggcc
     9001 gacgtccgca tcctgcaggc gctgcagtcc tcggcgcggt tcggccgcgc ggtgccgatc
     9061 gatccggtgc cgcggcccag gcggcccgac ctcggccagg agatccggat ccctgcgcac
     9121 gagatgccgc cgctggtcga cgtcgagcag ccggcgaagt gaacgagccc ggcgctactt
     9181 gacctcgatc gcggtcagcc cgcgcaggtc gccggccgcg ttccagccgt cgccggtctt
     9241 cttgtagacc ttgagccgca gcatgccctg cttgttgagc ttgacgaacg gcacgctggt
     9301 gtcgctgacg tcggccttgt cgagcgtcag gctgttgccg ctggcgtcgc ggagcgccag
     9361 gtgctcgaac gtcttcacgc cggtggcctc gagcagcgcg gcgagccgcc agcccttctg
     9421 atcggggctg ccgggcgtgc cctcgcgcgg cagggcgagc acctgcgggc cggtcagcga
     9481 gctcatgccg cccgcggtct tgaccgcgat gaccagctgg gtcggatcgg cggtggtccc
     9541 gccgctgtcg tcgttctggc cccggttgcc gccctgcgcg agcttgatcc ggatcgcggt
     9601 gacgtggtcc tcgcgcatcg ccggcttgcc cttgcgcgcg agctcgaccg ggtcgaacat
     9661 gccgaacgcc ggggcgccgc cgtcgccggg gaacaccgcg gggaccatgt cggggaagtt
     9721 ggtcgacggg tgggtgagct cggtcggcgt gggcttgccg ccctgcagcg tcaccgcgtc
     9781 ccaggtcccg agcttgcggg cgttgccggg gaccagcgcg tcgagccgcg gccagttcgc
     9841 gatcagcgac ggctggaccg acccgatggt caggtcgttg acgaacagct cgacgccgcc
     9901 agggacgacg gtcgcggcca ccgacgagcc ggggccggag ccggagccag ggatgatcga
     9961 ggcggcggtc gagcccgagc cctgggccgg cggcgcgggc ttgtcgccgt ggcaaccgga
    10021 ggcgagcgcg gcgagcgcga acaaacacag cggggtgcgc atcgcgcggg agggtatcac
    10081 gtcgtgtacg atggcccggc gatggagaag ctggcgcagg agatcgtccc catcctcgtc
    10141 ctggtcgcgg tcatcgcggt cgtggtcgcg cgcctgccgc gcgtcgacgt cagccacacg
    10201 ccggcgttcc ggcgccgccg cacgctcaac tgggtgccgc tcgggttgac ctacgcgttc
    10261 ctctacatgg gccgctacaa cctcgcggtg ctgaagaacg tgggcggcat cagccagcac
    10321 gactacggca acatcgacgg ctggggctcg gtggtctacg gcgtgtcgtt cctggtcaac
    10381 ggcccgctcg ccgaccggct cggcgggcgg cggacgatcc tgatcgcggc gggcgggtcg
    10441 ctggcggcca atgcgctgat cggcgtgctg tggaagaccg gcgcgatcga caaggacgcg
    10501 atcacggcgc tgaccgtgct gttcgtgctc aacatgtact tccagagctt cggcgcggtc
    10561 tcgatcgtca aggtcaacgc gtcgtggttc cacctgcgcg agcgcgggac gttcggcggg
    10621 atcttcggga tcctgatctc gctcggctac tacttctcgc tcgactgggg gcgccggatc
    10681 gccgacgcgg cgtgccccgc gtgcggtccg tacgccaacg gcgaacccga cgcgcccgct
    10741 ccgacgctgg ccggcagcct gggactggcc tggttgttcc tggtcccggc ggcgatcctc
    10801 gcggtgttct gggcgctgtc gttcctgtgg gtgcgcgaca agccgtcgga cgcggggttg
    10861 tcggatttcg acgtcgccga cgcgtcgagc ggacagcgcg agacccgcga gccggcggcg
    10921 caggtgatct ggcggatgct gaagcatccg gtgatcctga cgatcgcgat catcgagctg
    10981 tgctcggggt tcctgcgcca ggcgatcacc aagtggggcc cggacttcgc ccgtggcgtc
    11041 ggcgccggtc agagctacgt caaccagcac tggggcatgg tgacctgcat cgccgggatc
    11101 accggcggga tgttcgccgg cgtgatctcg gaccacctgt tccagtcgcg gcggccgccg
    11161 atgtcgaccg tgctgtacct gatcatgctg gtcggggcgc tggcgatcct cccgctgctc
    11221 ggcgcgccgg tggtggtcgg ctggatcgtc gcgttcatgg cgatggcgat catcggcgtg
    11281 cacggcatgc tgtcgggcgt ggcgagccag gacttcggcg gccggctcaa caccggcgtc
    11341 gcggtcgggc tgatcgacgg cttcgtctac ctcggcagcg cgatccagga ccaggtctac
    11401 ggcggggcct tgagcctgtt gcccgagaag ggcgacaagg cggccaggct gatcgagagc
    11461 tggcgggcct ggccggtcgc gatggtgccg gccgcggcga tcgggctcgc gctgtcgatc
    11521 cggatctgga acgcgcgggt ggtgcggccg gcggcggccg cggcgagccg gtgaccgccg
    11581 gcgatggcgc gcgcgaagtc gctgtcacgg gccggggtgg agcgcgagcg tgggtgtgag
    11641 cgcgaaaaga tcgatgccgt cggcacgctg ctcacccagg tctcgagcgc gctgttcatc
    11701 accgggcccg ggctgtcgat cgagtccggg ctgccgcact accgcgggat ccccggcctg
    11761 gtgcgccgga ccgcccagga cgcgcggatg atcgagtcgg cgctgtcgat cgacacgctg
    11821 cacaagaagc cgaagctgac ctggcgcacc ctgctcgaga tcgatcgccg ggtgtgcgag
    11881 gccgagcccg gccgcggcca cgaggtgctg gtcgcgctcg agcgccggct ggagcgcgcg
    11941 acgatcatga cggtcaacgt cgaccggctg caccagcgcg ccggctcgcg cagcgtgatc
    12001 gagatgcacg gcgcgctgca cgacctgcgc tgcgcgcgct gcgagatctc gacccgccac
    12061 gaccgctatg cgggcctgcc gctgccgccg ctgtgcgcgg cgtgtggctc ggtgctgcgg
    12121 ccggacatgg cgctgttcgg cgagccgctg ccgcccgacc cgttcacccg gctgcaggcc
    12181 gagctcgaga tcggcttcga catcgtgttc gcgatcggcg tcgtcgcggt gttcccctac
    12241 ctcgcccggc cggtgctgct cgccaagcag gaggggatcc cgaccgtcga gatcgcccac
    12301 gcggcgaccg agatctcgga gatcgtggac ttcgggctgc gcggctcgcc gggccggatc
    12361 ctcgagctga tctgggaggt gttcgcgcag ctggccgggc ggaggccggg ctgatccgca
    12421 cgatccggcc tgtggtaccc gcgctcagcg gccgaatgac actcgcgttc ataaaatatt
    12481 cgatgcctct cgatctcgaa gattggtaat ccagatcgcg gcgagtcggt agcaagaagc
    12541 accttaccga ggagaaaatg ctcccagaga tttactgacg ccctaagtgt gccgatctgt
    12601 aggcggaaaa aaacgaagcg aaagccgggc ggctttgata cgtcgtgcgg tgatggcact
    12661 acaaaacaag ctcacgaccg cgtggatcct gagttttctg atcggctgtg ggacgagccc
    12721 cgcgaccggg agtggcgatg gacaggatcc gatgggcacc gagggcgggt cgccggacgg
    12781 ctccgggact ccgggcggcg gcatgacaac cggcagcacg acgccggtgt tcccgaccgc
    12841 gcacccccgg atctacctga cgccgaaccg cgcgcggctc gcggcggcgc tgtcggcggg
    12901 cacgccggcg gcgacccggt tcaaggcgat cgtcgaccag tgggtcggcg gcgccgacct
    12961 gtgggggttc tcggcgtgga acgcggcgct gatcggccag ctcaccggca acgcgacgta
    13021 ctgcaccaag gcggtggccg cgatcgagac ccaggtcagc accgcggagg gcaagatcgc
    13081 ggcgggccag gcgcccgagg tcgccgccga cagctatctc ggcgtcggcg acatgatcgg
    13141 cgacctcgcg ctggtctacg actggtgctt cgacaaggtc acggcggcgc agcgcacgcg
    13201 ctggctggcg tacgccgatc aggcggtgtg gaacgtgtgg cacgcgtcgg ccgcgaagtg
    13261 gggcagcgcg accatcccgt ggaccagctg gtcgaccgac aatccgatga acaactacta
    13321 ctactcgttc atgcgcgcga ccatgctgct cgggctcgcg gccaagggcg aggacagcca
    13381 ggccgacgcg tggatcaccg agttccacga caccaagctg atgaaccagc tgatgccgag
    13441 gttcaacgcc gacctggccg gcggcggctc gcgcgagggc accggctacg gcgtcgcgat
    13501 gcgccggctg ttcgagctct acgacctgtg gcagtcgacc accggcgaga acatcggggc
    13561 cgcgacgccg cacacccggc agtcgatgct cagcgcggtg caccagctgg tgccgacccg
    13621 cgaccggttc gcgccgaccg gcgaccagtc acgcgactcg accgcgtcgt tcttcgacta
    13681 ccagcgcaac tacctccagg agctgatcca ggcgttcccc ggtgacaagc tctcggcgcg
    13741 cgccaagacc cagctccagg ggtcgaacct gccgaccatg agcatggggt tcatggcggt
    13801 gtacgacttc ctgtacgacg acgccgcggt gaccgcggcc ccggtcgacc tcaacaccac
    13861 gtactacgcg tccggcatcg gcgagctcta cgcgcgctcg ggctgggaca cccacgcgac
    13921 ctgggtcaac ctgatcgccg gcccgtacac cgaggaccac gcgcaccagg accagggctc
    13981 gctgatgatc tacaaggacg gctggctggc ctacgacagc gtgatcgact cgcactccgg
    14041 cctgccccag gacaccaccg cgcacagcct ggtccggatc accagcggtg gggccccggt
    14101 gcgccaggtc gtgggcacca aaccccagct gtcgtcgctg caccagggga cggggtacac
    14161 gtacgccgca gccgacctga ccgcggccta caacggcaac agcgcggtgc agaaggttca
    14221 gcgtgaggtc gtctacctgc agcccgacgt cgtggtcgtg ttcgaccgcg tccagagcgc
    14281 cgctggcacg acgcagacgt ggcagctcgc ctcgccggcc gcggcatcga tcagcgggaa
    14341 cctggcatcg attgccagct ccggacacac gctgaaggtc cagcagatcg ccggcggctc
    14401 gatgagcgtg acgccgttca ccagcgtgtc gtcggattat gccggcggat tccgcctcga
    14461 cgagacgatg tccggcggcg acaaccgcta tctgcacgtg ctgtcgatcg acggcgccgc
    14521 gtcgtcggtg actcccgcgg gcgacgcgtc caacccgggt gtgacggttg ccatgtcgaa
    14581 tggccatacg gccacggtca cgttcgtgcg cgacaacaca ggcgcgacgc tcgttctcga
    14641 cggcgcgacg aagtcgctcg cggtgggcct cgaccgcctt ccggaatagg tccggttcgt
    14701 gctacccctg gctgtctcat gaaggcaatc tggggtgctg tggtggttct cgggatggcg
    14761 tcggcgtgtg gggttgacgg gccggagttc tcggccgacc acccgcggat ctacatgaag
    14821 agccggaagg acgcgctcgt cgccgccctc caggcggggc agccggaggc gaagcggttc
    14881 aaggacatgg tggacggctg ggtcggcggc ggggacgtct acaacttctt ctcgtggaac
    14941 gccgcgctgc tcggtcagct gaccggcgat cccaagtact gcgccgcggc gatcaaggac
    15001 gtcgagcagc aggtcaacga cgccaccagc caggtcaacg gcggccagct gcccttcgtc
    15061 gcgcacgaca gctacctcga cgtcggcaac ctgatcggca acctcgcgct ggtctacgac
    15121 tggtgcgcgg agtcgctgcc ggccgaccgc cgcggcgcct ggctgtcgta cgccgaccag
    15181 gcggtctaca acgtgtggaa ccccgacgtc gcgaagtggg gcaacctccc cgacgcgacg
    15241 tggtcgggct ttgcgatcga cgacccgtcc gacaactact tctactcgtt cctgcgcgcg
    15301 accatgctgc tcggcctggc cgcgcatgac gagacgccgc gggccaaggg ctggctgagc
    15361 gagttccggg acaccaagct cgccggcgag ctggtcccgg tgttcgacat gcagctcgtc
    15421 ggcggcggct cgcgcgaggg caccggctcc ggcgtgtcgc agcgcgacct gttcgagctc
    15481 tacgacctgt ggaaggcctc gaccggcgag ccgatcgccg atctcacgcc gcacacccgg
    15541 gcgtcgatgc tggcgatgat ccaccagatc gtgccgacgc tcgaccggat cgcgccgacc
    15601 ggcgatcagt cgcgcgactc gctcgcgcag ttcttcgact accaccgcag ctacctgcag
    15661 gacctgatcg cgctgtattc caccgacccc gtggcgccgc acgcccaggc cctgctggcg
    15721 agctcctcgc tgccgcagat gagccagcag ttcatgtacg cgtccgactt cctgtacgcc
    15781 aaccggaacg tcaccgcgac cgcgctcgac ggcctcggca ccgcgtactt cgcgcccggg
    15841 atcggcgagg tctacgcgcg ctcgggctgg gacaagcacg cgacctgggt caacctgatc
    15901 gccgggccgt acagccagag ccacgcccac caggatcagg gctcgctgat gatctacaag
    15961 gacggctggc tggcctacga cgtggtgatc gactcgcact cgggggtgaa cccgccggtc
    16021 caggcgacca gcgcgcacag cctggtgcgg atcgtcgacg cgagcggcaa gaccgtcgcg
    16081 cagcaggcca agaacctgtt gaacccggac gaccagtcga agcccgactc caaggtcgtc
    16141 gcgctgcatc gccccaaggg agccggcttc ctgcacgtcg ccgccgacct gaccgcggcg
    16201 tacggcgggc agtcgacggt ccagcaggtc cagcgcgaga tcgtctatct cgagccgagc
    16261 accgtcatcg tctacgaccg gctcgcgacc aaggtcggca cccaggtggt gttccagctg
    16321 gtcagtccgg tctcgccgcc gaccgccacc ggcaccaccc acacgttcac gacggccggc
    16381 cacacgctca cgctggagga cgtcggcccc gtcctgggca tgccggccat cttcgatttc
    16441 gcgaagagcg acagcagcga gttcatgggt gggttccggc tcgacgagcc cgcccccggc
    16501 ggcaacaatg tccggttcct ccacgttttg acgatcgacg gcgcggcgac cggcatcgtg
    16561 cagggcgata ccccggggga cagcgtgaag ttgacggtcg gcggccagga cgtggtcatg
    16621 acgttcaacc acgacgccat cggcggcacg ctgacgatcg ggacccagtc gttcccgctg
    16681 ggcgcgagcc tcgacccgct gcccgagtaa cgctcgtcat tcgctccgac gagggcggcg
    16741 ggatcgtgag gcggcggtgg cgggccggcc cgctcgcgat cagcgcgact gccctgccga
    16801 gcctcgcgcg cgacgatctg tatcacggtc caatcatgac gcactgccag gatctgcgag
    16861 cgggatcgcc atcgagaatg tcgtgcctcg cccgagcttg ctggcgacgg agatcgttcc
    16921 gctgtgcgcc tcgatgatgg ccttgcagat gtagagtccg agtccaatgc ccttcttcgc
    16981 atgttgcttc gcggaccagt agggttcgaa gatgtggggc agctcgtcgt cggcgattcc
    17041 gggtcccccg tccgccacct cgaacgccac ctggcgatct gccacgcggg cgcggacggt
    17101 gacgacatcg cctcgaccac agaacttgag cgcgtttccg atgaggtttc cgaacgcttg
    17161 catcaggcgc tcgcgatcgc cgcagatccg ggcgtcttgc acgggacagt cgcagacgag
    17221 atcgatcccc tgcttcctgg ccagctcctc gtgcgcctcg atcaccctcg tgaccagcgc
    17281 cgcgacatct tcgttcttca tcgccatggg caggcggccc gcccgcaggc tggcggcgtc
    17341 gagcaagtcg ccgagcaagt gccccatgcg gctcgccgcg cgatggatcg cttccacgtg
    17401 cttgctcgcc ttcggtccga tgtcgctgct ctcgagcgcc acggtggctc cgagatcgat
    17461 cgtggcaagc ggattccgca gatcgtgaga cacgatcgcg aggatctctt cccgtgcgcg
    17521 cgtcgcgtcc tgcgcatccc ggtagagacg cgcgttctcg atcgcatacc cggcgcgacg
    17581 tcccagctcc atcgcggtct cgagcttgga tggcccgtag ctgcggccgg actccgcacg
    17641 gatgagcgtg accgctccca acgtctcgcc gtgcgcgacg agcggcgcga cgatgaccga
    17701 cgagattccg agctcgcgca tcagccggag atgcgcggca tcgcgcgcga tgtgcacgag
    17761 ctgtgcgtcc gagaactccg ggtagatcat cgctcggccg gtccggatga cctcaccgat
    17821 cccacccgac gcctgaaggt cggacggata tcgccgcgac cactcacgga caaccgtaac
    17881 cttcgttcga tccgagtgcg cgatcgcgac ccgctcgatc tggccatcct tgagcacgtc
    17941 gacgacacac cagtcggcga gctcgggaac cgcgaggttg gcgagctgct ccagcgtcgc
    18001 catcatgtcg agcgagctcg cgagcagctt cgtggcgttc gacaggaacc gctgatccgt
    18061 gacgtccgtg gcggtgctga accagcggat gacctggccg ctctcgtccc gcacgggcac
    18121 cgcccgcgac aggaaccagc gatagatgtt gtcctggcca cgaatcggaa acgtgtcttc
    18181 ccacggctcc tcgcccgcga tcgcacgtcg gatggtctcg gtgacgcgct ccacatgctc
    18241 tggatgctga acacgctgcc agccccagcc ctcgacatcg gcgagctttg tgcccgtgta
    18301 atcgaaccag cgctggttga accagtacgc cgatccggtc gtgtcggcga tccacaccag
    18361 ctgcggaatg ttttcggcca gcgtgcggaa ctccagctcg cgctcgcgca atgcctgttc
    18421 gatgcgcttg tgtgccgtga cgtcgacggc caccgcgacc gcgccgatca cgttgccgtc
    18481 gcgcacgatc ggcgccgcca cggcatgcac gatcgcgtcc ttcgcggctg tgtgatcgtg
    18541 gatgagcacg tctcgctgat aggtgacgcc tcgcaccgcg gtgcgtaccg gaccgttccc
    18601 gacgtccagc cgcgcgccgc tacccgcgtc gcgcatgtcg agcgcctcga cgaggctcac
    18661 caccggttgc cgcgcgagct ggtcggagtc gtcgtaaccc agcaggtcga gccctgcgcg
    18721 attggctcgc gtgaccgctc gctcgtttcc gatgaagacc gccgcgggca tgctctcgag
    18781 gatcgccgac agctcggccg aggctctctc ggcagcttcc cggagcaagt gctggaagat
    18841 cgcactcgtc gcgcgattgg ccatcgcgct gagcaggcgc ttgtcctgca tcgagaactc
    18901 gctggcagtc agcgagccca tgtgcgcgac g
//
LOCUS       DASVOR010000350         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_500159,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000350 DASVOR010000000
VERSION     DASVOR010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500159"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VF516_07245"
     CDS             complement(<1..271)
                     /locus_tag="VF516_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107507.1"
                     /translation="MTRDLPARSRRPTGPTTLSSLAHSGQVSIAAPRSRLGLIVGASA
                     IVVGSAIGSALALRVQPSSPPPAAHSAETTAASPRAGAPIAAAAPG"
     gene            538..1422
                     /locus_tag="VF516_07250"
     CDS             538..1422
                     /locus_tag="VF516_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107508.1"
                     /translation="MRNRFDQFGKQMVRAVLEDFCTIETDAEVPAETRRIDLWFMRRE
                     ARASLPEHLGLLGTIAGRAGTLELFHNTPSIDELVACLIKQGEFRHCLPRRTAAPPLP
                     LPIQWIISAGRPGRGIEGLAFRPMTGWPSGVYEGPPLLWTRLVVVNELPVARSTLMLR
                     LLGAGAVLQRAIAELTALPPDAPERMLVLPILQRTRLAMSNPQQRTPDDEEFLMVTED
                     IYETWRRQTVQEGVQQGLEQSLEREQKLLLRLLRQRFGDQVDGEIERRLAAASAEQIE
                     VWHERVLSAGTLADLLAD"
     gene            complement(1438..2055)
                     /locus_tag="VF516_07255"
     CDS             complement(1438..2055)
                     /locus_tag="VF516_07255"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8107509.1"
                     /translation="MRFVLRSGVVACVAGLLASAAARADAVPPALAREFQAGVDAYRL
                     GRYDDARAHLEKAQAIDPRLPGPHRFLAAVAQAQRRWADCIAEARRALQLNPRSQELG
                     DTRRLHDSCRESDGRPAYHGDLGEAAAIAVTANVSGATVRIGGLRYGGTPVAPRLIKP
                     GVLRVDLEKSGWKPAHVTIDALPAIVTDIAVELEPLAPAARPATP"
     gene            2218..>2468
                     /locus_tag="VF516_07260"
     CDS             2218..>2468
                     /locus_tag="VF516_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107510.1"
                     /translation="MSLATRAPSSLSRGAAIALAAAGPVAIGGILAIRAHDPRPMVAA
                     PAIVFGVVAATAPALYIATAAAGHAPPLAAMVRAFGVALG"
BASE COUNT          338 a          889 c          902 g          339 t
ORIGIN      
        1 cgccgggcgc ggccgcggcg atcggcgcgc cggctcgcgg cgacgcggcg gtggtctctg
       61 cggaatgcgc agcaggcggt ggactcgacg gctggacgcg cagcgccagc gcgctgccga
      121 tcgcactgcc cacgacgatc gccgatgctc cgacgatcag gccgagccgc gaccgcggcg
      181 ccgcgatcga gacctgcccg ctgtgcgcca gactggacag cgtcgtcggc ccggtgggtc
      241 ggcgcgacct cgcggggaga tcgcgtgtca tcgcgggccc tcgcagcttc tcgatcggac
      301 gtcgtgcatg gtgtgttccg ccgccgcact ccccggccag cgcacacacc atatcaaagc
      361 gcgagaccgc cgtatcgtcc ggagctcgcc tcggcgccgc gtccctggtg gcaaacctcg
      421 gatcctgcac aattcttcga gcgcgctgat ggtttgtcgc gatttcgcac cagacccgtc
      481 aattcgaccg gtcggcggtt ccgcggcacc aacccggtgc ccacttcggt cttgccgatg
      541 cgcaaccgct tcgatcagtt cggcaagcag atggtccgcg ccgtgctcga agacttctgc
      601 accatcgaga ccgacgcgga ggtccccgcc gagacccgcc gcatcgacct ctggttcatg
      661 cgccgcgaag cgcgcgcgtc gctccccgag cacctcggcc tgctgggcac catcgccggc
      721 cgcgcgggca cgctcgagct gttccacaac acgcccagca tcgacgagct cgttgcatgc
      781 ctgatcaagc agggcgagtt ccggcactgc ctgccgcgcc gcacggccgc gccgccgctc
      841 ccgcttccga tccagtggat catctcggcg ggccggccgg gccgcggcat cgagggtctc
      901 gcgttccgcc cgatgaccgg ctggccgtcg ggcgtctacg agggaccacc gctgctgtgg
      961 acgcgcctcg tggtggtgaa cgagctaccg gtcgcgcgga gcacgctgat gctgcggctg
     1021 ctcggcgctg gcgcggtgct gcagcgagcg atcgccgagc tcacggcgtt gccacccgat
     1081 gccccggagc gcatgctggt gctacccatc ctgcaaagaa cgaggttagc gatgagcaac
     1141 ccacagcagc gaacgcccga cgacgaggag tttctgatgg tcaccgagga tatctacgag
     1201 acgtggcgcc ggcagacagt ccaggagggc gtccagcagg ggctcgagca gagcctcgag
     1261 cgcgagcaga agctgctgct ccggctgctg cgacaacgct tcggagatca ggtggacggc
     1321 gagatcgagc ggcgcctggc cgcagcatcg gccgagcaga tcgaagtctg gcacgagcgc
     1381 gtgctgtccg cgggcacgct tgccgatctg ctcgccgact gaacgacgcg acgcgagcta
     1441 cggtgtcgcg gggcgagcgg ccggtgcgag cggctcgagc tcgacggcga tgtcggtgac
     1501 gatcgcgggc agcgcgtcga tcgtgacgtg ggccggcttc cagcccgact tctcgaggtc
     1561 gacgcgcagg acgcccggct tgatcaggcg cggcgcgacc ggcgtgccgc cgtagcgcag
     1621 gccgccgatc cggaccgtcg cgccggacac gttggcggtg acggcgatgg cggcggcctc
     1681 gccgaggtcg ccgtggtacg ccggccggcc gtcggactcg cggcagctgt cgtggagccg
     1741 gcgggtgtcg ccgagctctt gcgaccgggg gttgagctgc agggcgcggc gggcctcggc
     1801 gatgcagtcc gcccagcggc gctgagcctg ggcgacggcg gcgaggaagc gatgcgggcc
     1861 ggggagccgg ggatcgatcg cctgggcctt ctcgaggtga gcgcgggcgt cgtcgtacct
     1921 gccgagccgg taggcgtcga caccggcctg gaactcgcgg gccagggcgg gcggcacggc
     1981 gtcggcgcgc gctgcggcgc tggcgagcaa gccggcaacg cacgccacga caccggaccg
     2041 gaggacgaaa cgcaccgtgc accgcacggt agcgcacccc ggccgggatc tccacgatgg
     2101 cacatgcaac gcccgcggcg cgtctgcgca agatctccac gaacgctcga cacggcctcc
     2161 cgatcgcctg cgccgcgggt gcaccgcggc cgggttgatc ggggcaggag gcctgcaatg
     2221 tccctggcaa ctcgagctcc gtcttctctc tcgcgcggcg cggcgatcgc gctcgccgcc
     2281 gccggtccgg tcgcgatcgg cggcatcctc gccatccgcg cgcacgaccc gcgcccgatg
     2341 gtcgccgcgc cggcgatcgt gttcggcgtg gtcgcggcga ccgccccggc gctgtacatc
     2401 gcgaccgcgg ccgccggcca cgcgccgccg ctggcggcca tggtgcgcgc gttcggcgtc
     2461 gcgctcgg
//
LOCUS       DASVOR010000351         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_120083,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000351 DASVOR010000000
VERSION     DASVOR010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120083"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF516_07265"
     CDS             complement(<1..250)
                     /locus_tag="VF516_07265"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEX8107511.1"
                     /translation="MAPLFLIGFMAAGKTSVGRAIASATGRRFVDLDDVIAAAGTPVA
                     TLVASDEPEFRRREAQALTRAIATGDGAVIATGGGAAGY"
     gene            complement(268..>1855)
                     /gene="pilQ"
                     /locus_tag="VF516_07270"
     CDS             complement(268..>1855)
                     /gene="pilQ"
                     /locus_tag="VF516_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEX8107512.1"
                     /translation="DVTRFGGPVRAVSSFRDRRTPHRVRVVAELASPATPSFDREAGV
                     IRWHFVGADIARRPVTVRPGIAQAGAPVGGFGAASTPIAQQSVAQLPPQGTRGRRIYH
                     GAPMDLRFKDAPIHDLLRLIADWGKVNIVVPDTINATVTVDLKRVPWDQALDVILSSH
                     QLWYRREGNIYRIASRKELDTEDEEEAKRREAAFKAEAPQPQLIPLNYAAAADLRAKL
                     EPLLSSHGKIAVDERTNQLIVTDVAGNRAQIARLAVSLDTQTPQISIEARIVEANSTF
                     LRQIGIQWGGSALAGVAGGNATGLIWPSTVAVTGGNTDGQTPAAGVANPSNFAINLPA
                     ATGTGEGGAIGLTLGSVAGNLNINLRLSALEDTGSVRIISAPKITVLNNKLASIASGV
                     SVPISTVSATGTQTQFVQADLRLDVTPTVSQNDCAISMNLVVTKNAPDFSNTGARGDP
                     TIQRREARTSMLVADGETSVLGGIYTRNTSLNYKKVPLLGDLPVLGWLFKNRREQDDR
                     TELLVFITPKITNRALLRCK"
BASE COUNT          243 a          631 c          698 g          283 t
ORIGIN      
        1 cgtaccccgc cgcgccgccg ccggtcgcga tcacggcacc gtcgccggtc gcgatcgccc
       61 gggtcaacgc ctgggcctcg cggcgccgga actccggctc gtcgctcgcg accagggtgg
      121 cgaccggggt gccggccgcg gcgatcacgt cgtcgaggtc gacgaaccgc cggccggtcg
      181 ccgacgcgat cgcccggccg accgaggtct tgccggcggc catgaagccg atcaggaaca
      241 gcggcgccac gacctgggta ctatgactca cttgcagcgc agcagcgcgc ggttcgtgat
      301 cttgggcgtg atgaacacga gcagctcggt gcgatcgtcc tgctcgcggc ggttcttgaa
      361 cagccagccg agcaccggca ggtcgccgag caaggggact ttcttgtagt tcaacgaggt
      421 attgcgggtg tagatcccgc ccagcacgct ggtctcgcca tcggcgacca gcatcgaggt
      481 gcgggcctcg cggcgctgga tcgtcggatc gccgcgcgcg ccggtgttgc tgaagtcggg
      541 cgcgttcttg gtgaccacca ggttcatcga gatcgcgcag tcgttctgcg acaccgtcgg
      601 cgtgacgtcg agccggaggt cggcctggac gaactgggtc tgggtcccgg tcgccgacac
      661 cgtcgagatc gggaccgaca cgccggacgc gatgctcgcc agcttgttgt tgagcacggt
      721 gatcttgggc gccgagatga tccggaccga gccggtgtcc tcgagcgccg acagccggag
      781 gttgatgttg aggttgccgg cgaccgagcc gagcgtcagg ccgatcgcgc cgccctcgcc
      841 ggtgccggtc gccgccggca ggttgatcgc gaagttcgat ggattcgcga cgccggcggc
      901 cggggtctgg ccgtcggtgt tgccgccggt gaccgcgacc gtcgacggcc agatcaggcc
      961 ggtcgcgttg ccgccggcca cgccggcgag cgcgctgccg ccccactgga tgccgatctg
     1021 gcgcaggaac gtcgagttgg cctcgacgat gcgggcctcg atcgagatct gcggggtctg
     1081 ggtgtcgagc gacaccgcga gccgggcgat ctgcgcgcgg ttgccggcga cgtcggtgac
     1141 gatcagctgg ttggtgcgct cgtcgacggc gatcttgccg tgcgacgaca gcagcggctc
     1201 gagcttggcc cggaggtcgg cggccgcggc gtagttgagc gggatcagct ggggctgcgg
     1261 cgcctcggcc ttgaacgcgg cctcgcggcg cttggcctcc tcctcgtcct cggtgtcgag
     1321 ctccttgcgc gacgcgatcc ggtagatgtt gccctcgcgg cggtaccaca gctggtgcga
     1381 cgacaggatc acgtcgagcg cctggtccca cggcacgcgc ttgaggtcga ccgtgaccgt
     1441 ggcgttgatc gtgtcgggca cgacgatgtt gaccttgccc cagtcggcga tcagccggag
     1501 caggtcgtgg atcggcgcgt ccttgaagcg caggtccatc ggcgcgccgt ggtagatccg
     1561 ccgcccccgg gtgccctgcg gcgggagctg cgcgaccgac tgctgcgcga tcggcgtcga
     1621 cgccgcgccg aacccgccga ccggcgcgcc ggcctgcgcg atcccgggac ggaccgtgac
     1681 cgggcgcctg gcgatgtcgg cgccgacgaa gtgccagcgg atcacgccgg cctcgcggtc
     1741 gaagctcgga gtcgccggcg acgccagctc ggcgaccacg cggacccggt ggggcgtgcg
     1801 gcggtcgcgg aacgacgaca ccgcgcggac cggaccgccg aaccgggtga cgtcg
//
LOCUS       DASVOR010000352         3809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_295579,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000352 DASVOR010000000
VERSION     DASVOR010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295579"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1099)
                     /locus_tag="VF516_07275"
     CDS             complement(230..1099)
                     /locus_tag="VF516_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107513.1"
                     /translation="MKTAEQRVARLAVKREVLAKRYQDELEQIDRLKNQRASWRRDRE
                     LRDSLSESLETANQLSAATRDLGQARAELESARRAYLVAIDAELAAGAPPARAQQLTR
                     ARAQLAPQIKDIPRRIVIPDFEVDPLADPEELEQRAAELRASEEDLGRQLAGLKAQAT
                     ELDHLALLRKQHERAGDLFNRDDDEPHHNTARPGNDPGTAPDDGGGGAGKPSAPGGVS
                     GNFEAFVPIVLADVINASTLLSLEAAHRSGDPAQRADAARRAGDEVARRISEVHKRRL
                     EIEARARQLRAKS"
     gene            complement(1315..1695)
                     /locus_tag="VF516_07280"
     CDS             complement(1315..1695)
                     /locus_tag="VF516_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107514.1"
                     /translation="MLLLDERIKADGSHTRTWATCEGDRVRMIDDDGVVGELSVVAVD
                     QVMRRYGRPLDPEIALEGDSLTCGTCKLRRLRHRAQVDAEGRDYLVWERSDDEPLVCV
                     AATVTAALRFLVARWREQSIRNLR"
     gene            1961..3682
                     /locus_tag="VF516_07285"
     CDS             1961..3682
                     /locus_tag="VF516_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107515.1"
                     /translation="MRFLPSQIETTWKFQGDARSAKIRIYADAEVRALPHWKEDIGDQ
                     IDYANQLLEPMIGLRLTVDSYKDWARTGTPEDALRELTTLDKGDGVMWVIGYVAPATA
                     SSTVMSVLGDARPLGHHLTVRAWAEKPETEALAGRLPDPKDPDRVEVINAHHRHKQTV
                     VLLHMLAATLGAIAENDPSWIQAVSYSPQQSTFANRTRDLLQLAINSRLSDDTDEQLA
                     RKLDDDIEKTPWGGWVKPSYDEVVATIKNVIDAGKAGKTAAGIPTAAYDEYRRISELA
                     KRGQTADALIELDNLLTAYPGNATMHELKCEVMLTKPGVADPATRAACNRVAELAAGD
                     PTVHMAVGEALIRAGDIAGAHAELVLAEDKIGNLPTGAGEAWSRVIAAYTGLGALTWT
                     EQALTKAKLEASPAAAQVAQTRARYGIPRGARFVAPDQEAALVSAIRNALKLVYSSKF
                     GDADRAIAAAAKQWPNAPGLIAARCDLDFRMGQIETARATCARALAADPEDSWALYLL
                     GTLLLRDAGTTPAGIDKLKHAITVDPELGQAWRTLATASARGNDKAALDQLSKNYQAK
                     FGQALPP"
BASE COUNT          573 a         1441 c         1247 g          548 t
ORIGIN      
        1 cccccccccc ctgggatcga ctggccggtc cgtgtcctgg ccgcggccgc gcgctggcca
       61 gcgaatggcc accgtgacca acctccgacc caccgcgatc ggagcgttga tccaacgggc
      121 cttcgccccg ccccaccggc aacgccgtcg gggtccccac ccctttggcc cacggggctt
      181 cgccccgcgc cagcgatgcc gtcgggggcc ccacccccca tcgcgccgtt caggacttgg
      241 cgcggagctg gcgcgcccgc gcctcgatct ccaggcggcg cttgtggacc tcgctgatcc
      301 gccgggcaac ttcgtcgccg gcccggcgcg cagcgtccgc ccgctgcgcc gggtccccgg
      361 agcgatgtgc cgcctccagc gagagcagcg tcgaggcatt gatcacatcg gccagcacga
      421 tgggcacgaa ggcctcgaaa ttcccggaca ccccgcccgg cgcactcggc ttgccggccc
      481 caccgccgcc gtcgtccggc gcggtgccgg ggtcgttgcc cggccgcgcc gtgttgtggt
      541 gcggctcatc atcatcgcgg ttgaacagat ctccggcgcg ctcgtgctgc ttgcgcagca
      601 gcgcgaggtg gtcgagctcg gtcgcttgtg ccttgagccc cgcgagctgc cgcccgagat
      661 cttcctcgct ggcgcgcagc tcggccgccc gttgctccag ctcctccgga tcggccagcg
      721 gatcgacctc gaaatccgga atcacgatgc gccgcgggat atccttgatc tggggggcca
      781 gctgcgccct ggcacgggtc agctgctgcg cccgcgccgg cggtgcaccg gcggcgagct
      841 cggcgtcgat cgccaccagg taggcgcggc gcgcactctc cagctccgcg cgcgcttgcc
      901 ccagatcccg ggtcgccgca ctcagctgat tggcggtctc cagcgactca gaaaggctat
      961 cccgcaattc tctgtcgcgc cgccacgagg cgcgttgatt ttttaaacga tcgatctgct
     1021 cgagctcatc ctggtagcgc ttggcgagga cctcgcgctt caccgccagc cgcgccacac
     1081 gttgctcggc ggttttcacc gtcgtttcgg tcgtggaagt caccgcgacc ggcgccgatg
     1141 gcgggcgagg ttccgccgat gcggtggagc tcgccaccgc gatcgccagg accagcaacc
     1201 atcgactcat gacttcttcc attttgtagc agcctgcatg ccacgctcaa gcacggatgg
     1261 acggcggggt ggagtggcag aagttcgcgc tgaaggtgcg ttccgacgct gtcgtcatct
     1321 cagattcctg atgctctgct cgcgccatcg cgccacgagg aagcgcaatg ctgcggtgac
     1381 cgtggcagcc acgcacacca gcggttcgtc gtcggaccgc tcccacacca ggtagtcccg
     1441 gccctcggca tcgacctgcg cgcgatggcg caaccgccgc aatttgcagg ttccacatgt
     1501 gagtgagtcg ccttcgagtg caatctcggg gtcgagcggg cgaccgtagc gccgcatcac
     1561 ctggtcgacc gcgaccacgg acagctcgcc gaccacgcca tcgtcgtcga tcatccgcac
     1621 gcgatccccc tcgcaggtgg cccaggtccg cgtatgcgac ccatccgcct tgattcgctc
     1681 gtcgagcaac agcacgcgcg cgcacctcct ccctcctagg aggtcggtcg cccgccccgg
     1741 tcgcacgcaa atcgactgtg agacgctgca cgtcgtgtac gtcgcgtgcc gcacgaagtt
     1801 tgcgctccct ggccgctggc tgcacggtcg gtggcttgcg cgagccgggt tcgccgggga
     1861 aagtcccccc atgcgctccg ccgcggtccc cctgcgaccg ctcctcctgc tcgccctggc
     1921 cgcctgcggt gccagccagg cagagcgtat tgccgcgaag atgcggtttc tgccctcaca
     1981 gatcgaaacc acctggaagt tccaggggga tgcgcgcagc gccaagatcc ggatctatgc
     2041 cgacgccgag gttcgtgcgc tgccccactg gaaagaggac atcggcgacc agatcgacta
     2101 tgccaaccag ctgctcgagc cgatgatcgg gttgcgcctc acggtcgact catacaagga
     2161 ctgggcgcgc accggcaccc cggaagacgc gctgcgcgag ctgacgacgc tcgacaaggg
     2221 cgacggcgtg atgtgggtga tcggctacgt ggccccggcg acggcatcct cgaccgtgat
     2281 gtccgtgctc ggcgacgccc ggccgctggg ccaccacctg accgtgcgcg cgtgggccga
     2341 gaagcccgag accgaggcgc tggccggccg gttgcccgac cccaaggacc ccgatcgcgt
     2401 cgaggtgatc aacgcgcacc accgccacaa gcagaccgtg gtgctgctcc acatgctcgc
     2461 ggccacgctc ggcgcgatcg ccgagaacga tccgtcgtgg atccaggcgg tgtcgtactc
     2521 gccgcagcag agcacgttcg ccaaccggac ccgcgacctg ctccagctcg cgatcaacag
     2581 ccggctgtcc gacgacaccg acgagcagct cgcccgcaag ctcgacgacg acatcgagaa
     2641 gacgccgtgg ggcggctggg tcaagccgtc gtacgacgag gtcgtcgcga cgatcaagaa
     2701 cgtcatcgac gccggcaagg ccggcaagac cgccgccggc atcccgaccg ccgcctacga
     2761 cgagtaccgg cggatctccg agctcgccaa gcgcggccag accgccgatg ccctgatcga
     2821 gctcgacaac ctgctcaccg cctatcccgg caacgcgacg atgcacgagc tcaagtgcga
     2881 ggtgatgctc accaagcccg gggtcgccga cccggcgacg cgcgcggcgt gcaaccgggt
     2941 cgccgagctc gcggccggcg atccgaccgt ccacatggcg gtcggcgagg cgctgatccg
     3001 ggccggggac atcgccggcg cgcacgccga gctggtcctc gccgaggaca agatcggcaa
     3061 cctgccgacc ggcgccggcg aggcgtggag ccgcgtgatc gcggcctaca ccgggctcgg
     3121 cgcgctgacc tggaccgagc aggcgctcac caaggccaag ctcgaggcca gcccggcggc
     3181 ggcgcaggtc gcgcagaccc gcgcgcggta cggcatcccg cgcggcgcca ggttcgtcgc
     3241 gcccgaccag gaggccgcgc tggtcagcgc gatccgcaac gcgctcaagc tggtctactc
     3301 cagcaagttc ggcgacgccg atcgcgcgat cgccgcggcc gccaagcagt ggccgaacgc
     3361 gcccggcctg atcgcggcgc gctgcgatct cgatttccgc atgggccaga tcgagaccgc
     3421 gcgcgccact tgtgcgcggg cgctggccgc cgaccccgag gactcgtggg cgctctacct
     3481 gctcggcacc ctgctcctgc gcgacgccgg caccactccc gcggggatcg acaagctcaa
     3541 gcacgcgatc accgtcgacc ccgagctggg ccaggcctgg cgcacgctcg ccacggcctc
     3601 cgcgcgcggc aacgacaagg ccgcgctcga ccagctctcc aagaactacc aggccaagtt
     3661 cggacaggcg ctgccgccct gaccggacgg ccgggcacga agcgctctcg ctgcgccgcc
     3721 tgcgccgtat actcgtcacg tgagcttcat gcgccggatg ttctccgccg actaccgcgc
     3781 cgcggtggcc gccgaggcgg ccggcgacg
//
LOCUS       DASVOR010000353         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_207921,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000353 DASVOR010000000
VERSION     DASVOR010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207921"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF516_07290"
     CDS             complement(<1..234)
                     /locus_tag="VF516_07290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEX8107516.1"
                     /translation="MKFLCDRCKTRYSIGDDRVRGKILKIRCKNCANVITVREGMSPE
                     GDGAPGDAAERARKAANAATEALDGHAVASRAAN"
     gene            complement(368..1573)
                     /locus_tag="VF516_07295"
     CDS             complement(368..1573)
                     /locus_tag="VF516_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107517.1"
                     /translation="MRPAAAIAMIAVLGVAGGAACGNRQRENREPPRDARRAPRVIEP
                     STDRVGPLPPYAIRAEGVGPYKLGEKLSVLLEQLPSGPQIALFEIPGVVHRSVIRAED
                     DTILVGGEQASTASFIAIVGPDVARAEGKIHVGSTRDELVAALGPPVEDPERAHDPRL
                     LTPSALPNLRVVLGGDRVTAIVVTGVSAASRQVYDCPRPATTERGVGSCLTGAGELIE
                     TSGDEVMIRAADGERTLATSPKFPGLVFAAPLRNAAEGRDELVVVTRVDEAAARSWWV
                     AAYRLEGTRLLRTVDPAQVYQLSNANARWIGSDLHDVELYLELFSRAEGIEVGGLLAT
                     HSASRGKQIADVVVISPAQVARHRGKTAAAEASDAGAPGASPAGRVTQGAAVRTAEPA
                     AADSESSRR"
     gene            1621..2022
                     /locus_tag="VF516_07300"
     CDS             1621..2022
                     /locus_tag="VF516_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107518.1"
                     /translation="MTDNPRLFKGLHSTMTIDRPRPHIVTMVIIGRDVGEHGDAPLRT
                     LDGELRAGPFALYIDARDTRGASIDVSNVWAQWLRRNRDQLLEVHMLTGSRFVQLTAD
                     FVRRFAELGDTMRIYTDGAAFDEALQLATQR"
     gene            complement(2492..2629)
                     /locus_tag="VF516_07305"
     CDS             complement(2492..2629)
                     /locus_tag="VF516_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107519.1"
                     /translation="MRRSDARDKDVVLGAAGCAVMAAWFAAARSAARFDQATSHLATP
                     E"
     gene            3146..3907
                     /locus_tag="VF516_07310"
     CDS             3146..3907
                     /locus_tag="VF516_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8107520.1"
                     /translation="MKIEKRTAARAKERSESEQLVLFKPRGGKRRGAGRPPKGKRAGS
                     PHKERPFLHARYPVHVTLRVVGAVGNLRRRCVYQAIREATLTTARRENFRIVQLSIQR
                     THVHLLVEADDKRALARGMQGFQISAAKHLNAAISKGKLGPRRQGVVFPDRYHAEIIT
                     SPRQARHALNYVANNWRKHGEDLRAPMSSWTIDWFSSAAMFTGWAEYGDEPFLWRGPP
                     TYDPLIVYQPRTWLLREGWKKSGSISCREVPSAKQ"
     gene            3996..4127
                     /locus_tag="VF516_07315"
     CDS             3996..4127
                     /locus_tag="VF516_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107521.1"
                     /translation="MLVEPERRLLLRVSSSSERCAGPDLEALPYGFVLADRDEIREA"
     gene            complement(4166..>4443)
                     /locus_tag="VF516_07320"
     CDS             complement(4166..>4443)
                     /locus_tag="VF516_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HEX8107522.1"
                     /translation="AMELYRGLRGWISGMCVPELVLDAPGGGGKVPIVPSYLEHLDHE
                     RVVVRTYRGIRVDYPQPRERDCTVAYDAIYFAGVPDDDDREGSAEAG"
BASE COUNT          692 a         1631 c         1485 g          635 t
ORIGIN      
        1 gttcgccgcc cggctcgcca ccgcgtgccc atccagcgcc tccgtcgcgg cgttcgcggc
       61 cttgcgagcg cgctccgcgg cgtcgcccgg cgcaccatcg ccctcgggcg acatcccttc
      121 gcggaccgtg atcacgttcg cgcagttctt gcagcggatc ttgaggatct tgccgcggac
      181 ccggtcatcg ccgatcgaat accgggtctt gcaccggtcg cacagaaact tcacgccgtg
      241 ctccgtcccc ccgcgccgcg gacgcgaccg gctcggacga ggcggatttc accgggcaac
      301 acgcggcaca attgtgccga aaaaaacggg gtcaggcaaa ggcctggccg acgacagcgc
      361 ccgcggctca gcgccgggat gactcggaat cggcggcggc gggctcggcc gtgcgaacgg
      421 ctgcgccctg cgtcacacgt ccggccggcg aggcccccgg cgcccccgca tcgctcgcct
      481 ccgcggccgc cgtcttgccg cggtgccgcg cgacctgtgc gggcgagatc acgacgacgt
      541 cggcgatctg cttgccgcgc gacgcgctgt gcgtcgccag caggcccccg acctcgatcc
      601 cctcggcccg gctaaacagc tccaggtaga gctcgacgtc gtgcaggtcc gatccgatcc
      661 agcgcgcgtt cgcgttcgac agctgataga cctgcgcggg atcgaccgtg cgcaacagcc
      721 gcgtgccttc cagccggtac gccgcgaccc accagctgcg cgccgccgcc tcgtcgaccc
      781 gcgtcaccac gaccagctcg tcgcggccct ccgccgcgtt gcgcagcggc gccgcgaata
      841 ccaggcccgg gaacttcggg gacgtcgcca gcgtgcgctc gccgtccgcg gcgcggatca
      901 tcacctcgtc gccgctggtc tcgatcagct cgccggcgcc ggtcaggcac gacccgaccc
      961 cgcgctcggt cgtcgccggc cgcgggcagt cgtagacctg ccgcgacgcc gccgacaccc
     1021 cggtcaccac gatcgccgtc acccggtcgc cgccgagcac gacccgcagg ttgggcagcg
     1081 cgctcggcgt cagcagccgc ggatcgtgcg cccgctcggg gtcctcgacc ggcgggccga
     1141 gcgccgcgac cagctcgtcg cgggtcgacc cgacgtggat cttgccctcg gcccgcgcga
     1201 cgtcgggccc gacgatcgcg atgaacgacg cggtcgacgc ctgctcgccg ccgaccagga
     1261 tcgtgtcgtc ctcggcccgg atcacgctgc ggtgcaccac gcccgggatc tcgaacagcg
     1321 cgatctgcgg ccccgacggc agctgttcga gcagaaccga cagcttttct ccgagcttgt
     1381 acggccccac cccctccgcg cggatcgcgt acggcggcag cggcccgacc cggtccgtcg
     1441 acggctcgat cacccgcggc gcgcgccggg catcgcgcgg cggctcgcgg ttctcgcgct
     1501 gccggttgcc gcacgccgcc cccccggcca cgccgagcac ggcgatcatc gcgatggctg
     1561 cagcaggtct caccacacct ccatatagac caagaccgcg tccgcggcca tgctcccgtc
     1621 gtgaccgaca acccgcgctt gttcaagggc ctgcactcga cgatgacgat cgaccgcccc
     1681 cggccgcaca tcgtcaccat ggtcatcatc ggccgcgacg tcggcgagca cggcgacgcg
     1741 ccgctgcgca ccctcgacgg cgagctgcgc gccggcccgt tcgccctgta catcgacgcc
     1801 cgcgacaccc gcggcgcctc gatcgacgtc tccaacgtct gggcccagtg gctgcgcagg
     1861 aaccgcgacc agctgctcga ggtccacatg ctgaccggct cgcggttcgt ccagctcacc
     1921 gccgacttcg tgcgccggtt cgccgagctc ggcgacacca tgcggatcta caccgacggc
     1981 gccgcgttcg acgaggcgct gcagctcgcg acccagcgct gacccggggg gcgcacactc
     2041 ggcgcatcgc cccaccaaac ggcgccgagc cggcatgggt gtaggccggc aagcgatgac
     2101 gacctcgcac cccgtaggac gtggccatct ggcatcggcg aaccagactg ccacgacgac
     2161 gcagcccgcg gcgccaagcg tggcctgttc gaaccaggtg gccgatccgg cattcgcgaa
     2221 caaggctgcc acgaccgcgc agctcgcggc gccgagcacg gtgtccttgc gtctgacatg
     2281 gccgccgagc ctcggaagat cacatgctgc ctgtggatgg tggctcggcc cgtgacctgc
     2341 gcgccgcagc cgcaccggtc gacctcacgc gacagctcac cggcctttgc gccaccaccc
     2401 acaggcccga cgcccgcgcg caggcctccg ggcaggagat ttttctctct ttgtccggaa
     2461 gagaggaggc aaaggaagcc agccacgccc gctactccgg cgtcgcgaga tggctggtgg
     2521 cctggtcgaa ccgtgcggcc gatctggcag cggcgaacca ggctgccatg accgcgcagc
     2581 ccgcggcgcc gagcacgacg tccttgtctc tagcatcgct gcgccgcacc tggagatcac
     2641 atattccggg cgggagattc tctctcttcc ggaagcgcgg tggaagagac ccacgcgtag
     2701 cattcgcgat cgagattcgg cggctttgca aggaagccct tcttcggtcc gaaaacacaa
     2761 ggaatcaaaa aatccaacca gggagccctc gcgcgggcgt cagcgctgtc agacctgtgg
     2821 gcagggcgcc tggtcgaccg tgtcggctgc ggcgcgctgt ccacaggtgt gacagcgggg
     2881 tcacgcgttc tgcagaacct cgtcgccggt gcggactgga tcgcggtccc gagatctcgt
     2941 ccccccacgt cgttggcctg cgcccgactc accacttccc ggacactctc gttttgtgtc
     3001 cagggcgtcc ttggaccgag atgtcaatca tctgtccgaa ctgcacccat gcctgacaat
     3061 gatgtccacg atcgatgaca attctctact ataaattacg gagaaatcaa ttgactgaat
     3121 aacgatctgc gtctacacaa gagacgtgaa gatcgagaag cgaaccgccg cccgcgccaa
     3181 ggaacgctcc gagtccgagc agctggtgct gttcaagccg cgcgggggca agcggcgcgg
     3241 ggccggtcgc ccgccgaagg gcaaacgggc cggctcgccg cacaaggagc ggccgttcct
     3301 tcacgcgagg taccccgtcc acgtcacgct gcgggtggtc ggcgccgtcg gcaacctgcg
     3361 gcggcgctgc gtgtaccagg cgatccgcga ggccacgctg accacggcgc ggcgcgagaa
     3421 cttccgcatc gtccagctca gtatccagcg gacgcacgtc cacctgctcg tggaagccga
     3481 cgacaagcgg gcgctcgcca ggggaatgca gggcttccaa atctcggcgg ccaagcacct
     3541 caatgcagcg atcagcaagg gcaagctggg tccgcgccgg cagggcgtgg tgttccccga
     3601 tcgctaccac gccgagatca tcacatcgcc gcggcaagcg cggcatgcgc tgaactacgt
     3661 cgccaacaac tggaggaaac acggggaaga cctccgcgcg ccgatgtcga gctggacgat
     3721 cgactggttc tccagcgcgg cgatgttcac cggctgggcg gagtacggcg acgaaccgtt
     3781 cctgtggcgc ggcccaccga cctacgaccc gctgatcgtg taccagccga ggacctggct
     3841 gctccgcgag ggctggaaga aatccggatc gatctcctgc cgcgaggtgc cgtcggcgaa
     3901 gcagtgagca ctcgacggct ggtagcggtg tctgggcggc ggcgcgcccc gcgtcggctg
     3961 gatggagacg gactggcgag ccaatcttcc cggcgatgct ggtcgagcca gagaggcggc
     4021 tgttgctccg ggtctccagc tcgagcgagc gctgcgcggg gccggacctc gaggccctgc
     4081 cgtacgggtt cgtcctcgcg gatcgagacg agatccgcga ggcgtgacga ggacggccgg
     4141 cgcggcgtgg cgcggcgcgg tcgggttatc cggcttcggc cgagccctcg cggtcatcgt
     4201 cgtcggggac gccggcgaag tagatcgcgt cgtaggcgac ggtgcagtcg cgctcgcgcg
     4261 gctgggggta gtcgacgcga atgccgcggt aggtccggac gacgacgcgc tcgtggtcga
     4321 ggtgctcgag gtagctcggg acgatgggga ccttgccgcc gccgccgggg gcgtcgagca
     4381 cgagctcggg gacgcacatg ccgctgatcc agccgcgcag accgcggtag agctccatcg
     4441 cgg
//
LOCUS       DASVOR010000354         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_324733,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000354 DASVOR010000000
VERSION     DASVOR010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324733"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF516_07325"
     CDS             complement(<1..303)
                     /locus_tag="VF516_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107523.1"
                     /translation="MPGIPGLRHRWLLVAGSLAAAAGFALSVQGGRWWSVSDVAVGPL
                     GARSPFGGLGGFSWAGGSAQWERFGVATYAAGMIAMLVLIFVAGALAARRVPRLAAK"
     gene            616..1338
                     /locus_tag="VF516_07330"
     CDS             616..1338
                     /locus_tag="VF516_07330"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8107524.1"
                     /translation="MLAVARMAGTLRVPLADCVIDQLVRWQHDLRDRLHGLEAGLVAF
                     ADQAGMAVPDLTVLSTEPVARTESSGFGWRDDPIRHTRAFHAGTDFRGQPGTPVMAAG
                     DGVVTFCGRMGGYGNVVDVDHGGGVVTRYAHLRRIATKKAAAVTAGDVIGQVGSTGRT
                     TGPHLHFEVRIDGAPVSPIAAMAVAQIQREVPIAGRLAALALSPELQANAESDVDPPK
                     QHARPAPRPERAGRSKRQQVLW"
     gene            1362..>3344
                     /locus_tag="VF516_07335"
     CDS             1362..>3344
                     /locus_tag="VF516_07335"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8107525.1"
                     /translation="MSRSQAARRYVVWVRNHTFAIILVHLALLAAALDLIGFHLPLYA
                     DFSYLLPQDAPAVRDLRRLEARVKANDIVLALVTAPSPDARAAAARELAEQVRTLPPD
                     LVESVISDDSEARAYFKAHRHLFVPLADLEKASAALERRIKAAKLAANPLYIDLDDPA
                     PDAERDRRELDELRAKRREAEARLDRSTTVSADGLIGMIQIGTSFRATDAGPGADLVA
                     RLDAIGARVTAHHPGVTIGFTGGAITAVAEHDAIFKGMVLSSVITTLLVALVLALYFR
                     SATLLVLLLGTLGIATTVAFGVAAITVGHLNAATAFLGAIIAGNGVNYGILLIARYLE
                     ERRTRDIDSAMAVAIRGTLRPTAVASLGAAIAYGSLAATSFRGFADFAAIGAIGMILC
                     WLASYVLLPALVLRWGAGKRSAALHPLIGSLLVRVFGFRRPRVVCGVALVGAVLATAV
                     VARYVANDPFEYDITQLRSEGADAITARAWMSLSDRTFGRGYSGRTYIAADRLDQVPL
                     IVRALRARDANVPPEHQTVGSVASILDAVPDDQPRKLAILGHIHALLDDEALDALPES
                     ERAELAELRPPADLGMITAQNLPKIVRDQLEEKDGRIGYIVSIRPAPRLDEWNGHDLI
                     RFADAVRSIHLGDGETVTTSGASVIFADIVTSISGDG"
BASE COUNT          456 a         1267 c         1162 g          459 t
ORIGIN      
        1 cttggcggcc agccggggga cgcgccgggc cgcgagcgcg ccggcgacga agatcagcac
       61 cagcatcgcg atcatgcccg cggcgtaggt cgcgacgccg aaccgctccc actgtgcgct
      121 gccgcccgcc cacgagaacc cgcccagccc accgaacggg ctgcgcgccc cgagcggacc
      181 gaccgcgaca tcgctgaccg accaccagcg gccgccctgc acagacagcg cgaagcccgc
      241 cgccgcggcg agcgacccgg cgacgagcag ccagcggtgc cggagacctg ggataccggg
      301 catgtcgagg gggggagttt gatcggcaaa ccccgcgatc gcaatgccat tctggcaatc
      361 gcccggcggg tcgacgaaat cggtctgcct acattgccag cgcgcgccag atcacaaaat
      421 agtcggcctg ctttgggacc gaccgtgcat cgtgtgtgcc gccttgccgc cggctgggtg
      481 gccgtctggg ccaccggggc gccgctgggg agcgccaccg cggcgccggc cggccacgac
      541 acggcggtgc agccggaggt agcggcatcg gtttcggcat cggccgcggc gccggccatc
      601 gaggataccc aggcgatgct ggcggtcgcc cggatggccg gcacgctgcg ggtgccgctc
      661 gccgactgtg tgatcgacca gctggtgcgc tggcagcacg acctgcgcga ccggctgcac
      721 gggctcgaag ccgggctggt ggcgttcgcc gatcaggccg gcatggcggt ccccgacctc
      781 accgtgctgt cgaccgagcc ggtggcgcgc accgagtcct cggggttcgg ctggcgcgac
      841 gatccgatcc ggcacacgcg ggcgttccac gcgggcaccg actttcgcgg ccagcccggc
      901 acgccggtga tggcagccgg cgacggtgtt gtcacattct gcggccggat ggggggatac
      961 ggcaacgtgg tcgacgtcga tcacggcggc ggcgtcgtga cccgctacgc gcacctgcgc
     1021 cggatcgcca ccaagaaggc cgccgcggtg accgcgggcg acgtcatcgg ccaggtcggc
     1081 tcgaccgggc gtaccaccgg gccgcacctt cacttcgagg tccggatcga tggcgcgccg
     1141 gtcagcccga tcgcggcgat ggcggtcgcg cagattcaac gcgaggtccc gatcgcgggg
     1201 cggctggccg cgctcgcgct gtcacccgag ctccaggcga acgccgagtc cgacgtcgat
     1261 ccaccgaagc agcatgccag gccggcaccg cgtcccgaac gcgccggccg atcgaagcgc
     1321 cagcaggttc tctggtagct ctcgtgccat agttggagtc cgtgtctcgc agccaggcag
     1381 ctcgtcgcta cgtcgtgtgg gtacgcaacc acacgttcgc gatcatcctg gtccacctcg
     1441 cgctgctcgc cgccgcgctc gatctgatcg ggttccacct cccgctgtac gccgacttct
     1501 cgtacctcct gccgcaggat gcccccgcgg tccgcgacct gcggcggctc gaggcgcggg
     1561 tcaaggccaa tgacatcgtg ctcgcgctgg tcaccgcgcc gagccccgac gcgcgcgccg
     1621 cggcggcccg cgagctcgcc gagcaggtcc gcacgctgcc gcccgatctg gtcgagagcg
     1681 tgatctccga cgactccgag gcccgcgcgt acttcaaggc gcaccgccac ctgttcgtgc
     1741 cgctcgccga cctggagaag gcgtcggccg cgctggagcg ccggatcaag gcggccaagc
     1801 tcgccgccaa cccgctgtac atcgatctcg acgacccggc gcccgatgcc gagcgcgacc
     1861 ggcgcgagct cgacgagctg cgcgcgaagc ggcgcgaggc cgaagcccgg ctcgatcgct
     1921 cgaccaccgt gtcggccgac ggcctgatcg gcatgatcca gatcgggacc agcttccgcg
     1981 ccacggacgc cggccccggc gcggacctgg tcgcgcggct cgacgcgatc ggcgcccggg
     2041 tcaccgccca ccaccccggc gtcacgatcg ggttcaccgg cggcgcgatc accgcggtcg
     2101 ccgagcacga cgcgatcttc aagggcatgg tgctgtcgag cgtgatcacc acgctgctgg
     2161 tcgcgctggt cctcgcgctg tacttccgca gcgcgaccct gctcgtcctg ctgctcggca
     2221 cgctgggcat cgcgaccacc gtggcgttcg gcgtcgcggc gatcacggtc ggccacctca
     2281 acgccgcgac cgcgttcctc ggcgcgatca tcgccggcaa cggcgtcaac tacggcatcc
     2341 tcttgatcgc gcgctacctc gaggagcgcc ggacccgcga catcgacagc gcgatggcgg
     2401 tcgcgatccg cggcacgctg cgaccgaccg cggtggcctc gctcggcgcc gcgatcgcct
     2461 acggctcgct cgccgcgacc agcttccggg ggttcgccga cttcgccgcg atcggcgcga
     2521 tcggcatgat cctgtgctgg ctggcgagct acgtgctgct gcccgcgctg gtgctgcgct
     2581 ggggcgcggg caagcgctcg gcggcgctcc acccgctgat cggctcgctg ctggtccgcg
     2641 tgttcggctt tcgccggccg cgcgtggtgt gcggcgtcgc gctggtcggc gccgtgctcg
     2701 cgaccgcggt ggtcgcgcgc tacgtggcca acgatccgtt cgagtacgac atcacgcagc
     2761 tgcgctcgga gggcgccgac gcgatcaccg cgcgggcctg gatgtcgctg tccgaccgca
     2821 cgttcggccg cggctactcg ggccgcacgt acatcgccgc cgaccgcctc gatcaggtcc
     2881 cgctgatcgt ccgcgcgctg cgcgcccgcg acgccaacgt gccgccggag caccagaccg
     2941 tgggctcggt cgcctcgatc ctcgacgccg tccccgacga tcagcccagg aagctcgcga
     3001 tcctcggcca catccacgcc ctgctcgacg acgaggcgct cgacgcgctg cccgagtccg
     3061 agcgcgccga gctcgccgag ctccggccgc ccgccgacct cggcatgatc accgcgcaga
     3121 acctgccgaa gatcgtgcgc gatcagctcg aggagaagga cggccggatc ggctacatcg
     3181 tgtcgatccg gccggcgccg cggctcgacg agtggaacgg ccacgacctg atccggttcg
     3241 ccgacgcggt gcgctcgatc cacctcggcg acggcgagac cgtcacgacc tcgggcgcca
     3301 gcgtgatctt cgccgacatc gtcacgtcga tctcgggcga cggg
//
LOCUS       DASVOR010000355         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_149373,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000355 DASVOR010000000
VERSION     DASVOR010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149373"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..221)
                     /locus_tag="VF516_07340"
     CDS             complement(<1..221)
                     /locus_tag="VF516_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107526.1"
                     /translation="MRISRTAFLIGIAMSGGCAAPDEAEAPTDPMVAQASRHGDEGCD
                     WPQWGSDPAHTGQACKGTRLKRIIAHRTF"
     gene            complement(1392..1826)
                     /locus_tag="VF516_07345"
     CDS             complement(1392..1826)
                     /locus_tag="VF516_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107527.1"
                     /translation="MTLPRAGRTGATPRQSANFTIVGTPSTRAERWYEFEIIHDGVSL
                     GNLGGQVDQIFKDQMLGVLKNEHGRKLGSIPRAVWKDLVGGDDAEYLGYAVLSSYNTM
                     YFFRDGPRLLHVVLANAHGVKLAHSQFDAEVFDCWRPQLRDF"
     gene            complement(2054..>2277)
                     /locus_tag="VF516_07350"
     CDS             complement(2054..>2277)
                     /locus_tag="VF516_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107528.1"
                     /translation="EFQLIQDKLAKMEVARMNLQNLVFRQIELAGAGKSLDLAEASAC
                     KLLRGTRRDGGRARGGTRPAGSIRPKEGA"
BASE COUNT          405 a          699 c          735 g          438 t
ORIGIN      
        1 tcgaacgtgc ggtgtgcgat gatgcgtttg agacgcgtgc ccttgcacgc ctggccggtg
       61 tgtgcgggat ccgatcccca ctgcggccag tcgcaacctt cgtcaccgtg ccggctcgcc
      121 tgagcgacca tcgggtcggt aggtgcttcc gcttcgtcgg gtgcggcgca gcctccggac
      181 atggcaatac cgatcaaaaa cgcggttctc gagatcctca tccttccctc cttgtccaca
      241 atggacacac caacctgcgc cgcggtagcc ggaacgctgg atcgggatcg ctggcgtccg
      301 atcacgattc gacgcagcga ggactccatt gcatctgatt cgagtcactg ccagcaacga
      361 gcgcagaact cgcgatagcc gcactctcga acatcgcgag tgcggcccgg ccgggcgaag
      421 tcccatgact ctccatcttc ctcaccgagg caccatgctt catgtatagc cgggcgcgcc
      481 gtgtggtgcg acttcatata taaccgggcg tgccgtgtgg tgcaacccca acttgattat
      541 tacttctgcc tgataagaga agcaaccatc tcgattcagc accggcctgt catttttgcg
      601 gtttccgggt ttcggtggtc gccggcgata cagtctctct ctcgcttggt ggcggcaaag
      661 gtgggaacgg atctgttgta gaacgtctcg agccagaatg ccttggcatt gcggcgatgg
      721 tggccagtgg cgcgagagct cgggcgacca gcacggagcg acagcgcaca gaccggtggg
      781 catgatcgat cgacgacgtt cgaaggaggc tagatcatgg cggagatgga tcgcgcggtg
      841 gtctaagcgc atgggacgat cagcatcgag cgacagcgca tgggtctaca tctgatgcgc
      901 cagttgtccg gccagctctc gattcatcga aggtgtaatg cggtcagtgg gggtgcaatc
      961 agctcgatga cgttgtagag agggccagat cgaggtgcgg gatagcgcag tgcgggatag
     1021 cgcagtgcgg gatagcgcag tgcgggatag cgcagctggt gggctggaga ggctgggacg
     1081 atcggcatgg agcgatcgcg cacagtccca ggtccgctcg tccggccgga gctcgggcga
     1141 cttcagctcg cctcgaaact gttgggtcta ttcgttggcg agcgcggcct cgagctccgc
     1201 gaccacgcgc agcgtcaggc atcggcgacg gctggcacat cgccagcctg gcacatcgcc
     1261 aacgcggcac tgagctcgta ggcaaagtcc ggcagcggcg gacctcgccg gcgcgaacga
     1321 ggtccgagcg ccacgccgcc gcgggcgacg accggctgcg cgacgaggcg ctgcgcgtgc
     1381 gtggcgagat ctcagaaatc tctcagctgc ggacgccagc agtcgaacac ctccgcatcg
     1441 aactgtgaat gcgcaagctt gactccatgg gcgttcgcca gcacgacatg gagcaatcga
     1501 ggcccatccc tgaagaagta catggtattg taactggata gcaccgcgta accgaggtac
     1561 tcggcgtcat cgccgccgac gagatccttc catactgcgc gaggtatgga gcccagcttt
     1621 cttccgtgtt cgtttttcag aactccgagc atctggtcct tgaatatttg atctacctgt
     1681 ccgcccagat ttccaagact cacgccgtcg tggatgatct cgaattcata ccaccgttct
     1741 gcccttgtcg acggggtgcc gacgatggtg aaattcgcag attgtcgggg tgtcgcacca
     1801 gttcttccag ctcgcggtag ggtcatcgcg gctcttgaga ggcgcgtgtt ccccgggggg
     1861 ctcgttcggt gggatgcaga gagcaccgcg ctgacgtgcg tacgagcaga ggtcctcgca
     1921 cttgccacag cgacgctcgc gtgttgcacc ggctgacagg tcacaggggt cgccgcgctc
     1981 gaggtcgccg cgctcagggt cgccgtcctc ggcgcatggg accggcgagg atctcctact
     2041 cccgagcgag ccgtcatgcg ccctccttcg gacggatact tcccgctgga cgggtaccgc
     2101 ctcgagcgcg acctccatcg cggcgcgtgc cgcgtaagag cttgcacgcc gacgcctcgg
     2161 cgagatccag cgatttgccc gcgcccgcga gctcgatctg gcggaagacg aggttctgga
     2221 ggttcatccg cgcgacctcc atcttcgcca gcttgtcctg gatgagctgg aactcgc
//
LOCUS       DASVOR010000356         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266143,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000356 DASVOR010000000
VERSION     DASVOR010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266143"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2091)
                     /locus_tag="VF516_07355"
     CDS             complement(<1..>2091)
                     /locus_tag="VF516_07355"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8107529.1"
                     /translation="PLTPPLRHPGPVTDAKIRGDGTRVLTVGGDVVRIWEVTANPPPP
                     IEIRHAARINAAVFSADGARIATASDDRTARLWSVVTGQPVIPPLVHDAKVLSVELSR
                     DGARLATIVSESHIASMRQSDAGGEITVREPRGAALWNARTGARIADLEVGPVPAAPG
                     AGGHISVATSAAFSADSRRLVTTGTDRVALVWDATTGKTILPSLPHDAYVSSAAFSAD
                     GARIVTVDVLGCARLWDAATGAAAKLATDATAVARVTYSRGGTRMLVLCTDKRVRIWD
                     AASTRELAVPELESSLSDAAITADGARVATASADGVARIWDVRPAMRVLQVVPGAFDA
                     AYSPDGSRIATTAADGGLWLWTAAGKLVAQRKSFTLGAASPVFDRSGTQIAVIAVDGT
                     IEVGSAATATLRGLGDVQGKNPTGATDDPDDIYGHLPPRGAFSPDGRRFATLAGAHQA
                     TIWDIAAGEPVTPPLQHAGPVLMVRWSPDGRRVITASEDATARLWDAATGAAIGAPLA
                     HPADRWVTAALFTPDGKRIVTLCTDNNARLWDAASGQLQAVLSGHSAYIIAAEISRDG
                     TRLLTHSTDHSARLWNLATGKLAIPPLEHDDWVLDAGLSPDGARVVTASDHLVQVWDA
                     RSGLPLAPPFVHAEVARTAMFSPDGASVLSVEAAAIEIWDAGLDTGSLEDWHRLARDG
                     SFPQLGQIVGRARS"
BASE COUNT          238 a          793 c          775 g          285 t
ORIGIN      
        1 tccgatcgcg cgcggcccac gatctgcccg agctgcggga acgagccgtc gcgcgcgagc
       61 cggtgccagt cctcgagcga gccggtgtcg agcccggcgt cccagatctc gatcgccgcg
      121 gcctcgacgc tgagcaccga ggcgccgtcc gggctgaaca tcgccgtgcg cgcgacctcg
      181 gcatgcacga acggcggcgc cagcggcagg ccggaccggg cgtcccagac ctgcacgagg
      241 tgatcgctcg cggtgacgac ccgtgcgccg tcggggctga gcccggcgtc gaggacccag
      301 tcgtcgtgct ccagcggcgg gatcgcgagc ttgccggtcg ccaggttcca cagccgcgcc
      361 gagtgatcgg tgctgtgggt gagcagccgc gtgccgtccc gcgagatctc ggcggcgatg
      421 atgtacgccg agtggccgga cagcaccgcc tggagctggc ccgacgccgc gtcccacagc
      481 cgcgcgttgt tgtcggtgca cagcgtgacg atgcgcttgc cgtcgggcgt gaacagcgcc
      541 gcggtcaccc agcggtccgc ggggtgagcg agcggtgcgc cgatcgccgc gccggtcgcc
      601 gcgtcccaca gccgggcggt ggcgtcctcg ctcgcggtga tcacacggcg gccgtcgggg
      661 ctccaccgga ccatcagcac cggcccggca tgctgtagcg gcggcgtcac cggctcgccg
      721 gctgcgatgt cccagatcgt ggcctggtgc gcgcccgcca gcgtcgcgaa ccggcggccg
      781 tcggggctga acgcgccgcg cggcggcagg tggccgtaga tgtcgtcggg gtcgtccgtc
      841 gcgcccgtcg ggttcttgcc ctgcacgtcg cccagtcccc gcagcgtcgc ggtggcggcg
      901 ctgccgactt cgatcgttcc gtcgactgcg atcacggcga tctgcgtgcc gctgcgatcg
      961 aacaccggcg aggcagcacc gagcgtgaac gacttgcgct gcgccacgag cttccccgcc
     1021 gcggtccaca gccacagccc gccgtcggcc gccgtcgtcg cgatccgtga tccgtcgggg
     1081 ctgtacgcgg cgtcgaacgc gcccgggacg acctggagca cccgcatcgc cggccggacg
     1141 tcccagatcc gggccacacc gtcggcgctc gccgtggcga cccgcgcgcc gtcggcggtg
     1201 atcgcggcgt cgctcaggct ggactccagc tccggcaccg cgagctctct cgtgctcgcc
     1261 gcatcccaga tccgcacccg cttgtcggtg cacaggacga gcatccgcgt gccgccccgg
     1321 ctgtaggtga cgcgggcgac cgccgtggcg tcggtcgcga gcttcgcggc ggcgccggtc
     1381 gccgcatccc acagccgcgc gcagcccagg acgtcgacgg tgacgatccg cgcgccgtcc
     1441 gcgctgaacg cggcggacga tacgtaggcg tcgtgcggga gcgacggcag gatcgtcttg
     1501 ccggtggtcg cgtcccacac gagcgcgacg cggtcggtcc cggtggtgac caggcgccgg
     1561 ctgtccgcgc tgaacgcggc gctggtcgcc acgctgatgt gtccccccgc gccgggtgcc
     1621 gccgggaccg gacccacctc cagatcggcg atccgcgcgc cggtccgggc gttccacagc
     1681 gcggcgccgc gcggctcgcg caccgtgatc tcgccccccg cgtcgctctg gcgcatcgag
     1741 gcgatgtggc tctcgctcac gatggtcgcg agccgcgcgc cgtcgcggct gagctcgacg
     1801 ctgagcacct tggcgtcgtg aacgagcggc gggatcaccg gctggccggt gacgacgctc
     1861 cacagccggg cggtccggtc gtcgctcgcg gtcgcgatcc gcgcgccgtc ggcgctgaac
     1921 accgccgcgt tgatccgggc cgcgtggcgg atctcgatcg gcggcggcgg gttcgcggtc
     1981 acctcccaga tccgcacgac gtcgccgccg accgtcagga cccgcgtccc gtcgccgcgg
     2041 atcttcgcgt cggtcaccgg cccggggtgg cgcagcggcg gcgtcagggg t
//
LOCUS       DASVOR010000357         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_120127,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000357 DASVOR010000000
VERSION     DASVOR010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120127"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF516_07360"
     CDS             <1..542
                     /locus_tag="VF516_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX8107530.1"
                     /translation="VVWRGPMVHRLLQQFLGDVDWGELDYLVCDLPPGTGDVQLSLSQ
                     LIPIAGAVMVTTPQEVAIIDVVKGIAMFEKVEIPILGIIENMSYYKCPACGHSDEIFS
                     HGGGKRLAQEVGAEFFGEIPIDTRIRFGGDAGVPIVIASPDSDNAQRFMDLASKAALK
                     IAGNVLSKPKRSPRLAVIK"
     gene            complement(565..1080)
                     /locus_tag="VF516_07365"
     CDS             complement(565..1080)
                     /locus_tag="VF516_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107531.1"
                     /translation="MTQPIPELEAQVLRAGWDLTGMEHASLTGMMRFPFLVSLVTAVA
                     ALGAGCVVATAPARPAEEHAMRNGFYYLGERMVNGGVDHDVIHVGRRVGRFHEIMIVV
                     ERAPIELFDLVVTFGNGERYEPRTRLVFGADSTSRNIDLPGGARIIQRVDFRYGNLVA
                     GAQAKVELWGR"
     gene            1271..>1859
                     /locus_tag="VF516_07370"
     CDS             1271..>1859
                     /locus_tag="VF516_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107532.1"
                     /translation="MLLVAAACGHPGNGARSAVAPPADPGQPTRDAEIAARAAPFVDA
                     YSNYGAVLAPDGQVVFVSNRDGLPQLYVGALGEPDRPPRRLPVPRERVAAPRLLPDGK
                     TLVFLSDVGSDEKYHVYRIGLDGSGLTDLTPDGDLRRMTPQIARRSGAMLYAAHVLDD
                     QATRVFAHTVDDPPREIYRDPKVGFIADVSADGARA"
BASE COUNT          282 a          616 c          676 g          285 t
ORIGIN      
        1 ccgtggtgtg gcgcgggccg atggtccatc gcctgctcca gcagttcctg ggcgacgtcg
       61 actggggcga gctcgactac ctggtgtgtg acctgccgcc gggcaccggc gacgtccagc
      121 tctcgctgtc gcagctgatc ccgatcgccg gcgcggtcat ggtcaccacg ccgcaggagg
      181 tcgcgatcat cgacgtggtc aagggcatcg cgatgttcga gaaggtcgag atcccgatcc
      241 tcgggatcat cgagaacatg agctactaca agtgccccgc gtgcggccac tccgacgaga
      301 tcttcagcca cggcggcggc aagcggctcg cccaggaggt cggcgccgag ttcttcggcg
      361 agatcccgat cgacacccgg atccggttcg gcggggacgc cggcgtgccg atcgtgatcg
      421 cgagcccgga ctcggacaac gcgcagcggt tcatggacct ggcgtcgaag gccgcgctga
      481 agatcgccgg caacgtgctg tccaagccca agcgctcgcc gcggctggcg gtgatcaagt
      541 agcgccgcgc cggtcaccgg ccggctatct accccagagc tcgaccttgg cctgcgcacc
      601 ggcgaccagg ttgccgtagc ggaagtcgac gcgctggatg atgcgcgcgc cgcccggcag
      661 gtcgatgttg cgcgacgtcg agtccgcgcc gaacacgagg cgggtgcgcg gctcgtagcg
      721 ctcgccgttg ccgaacgtga ccacgaggtc gaacagctcg atcggcgcgc gctcgacaac
      781 gatcatgatc tcgtggaacc ggccgacgcg gcgcccgacg tggatgacgt cgtggtccac
      841 gccgccgttg accatgcgct cgccgaggta atagaagccg ttgcgcatcg cgtgctcctc
      901 ggcggggcgc gccggcgcgg tcgcgacgac gcagcccgcg cccagcgcgg cgacggcggt
      961 gacgagggaa acgaggaatg ggaatctcat catgccggtg agactcgcat gttccatgcc
     1021 agttaaatcc cagccggccc gcagaacctg cgcttccaac tccggaatag gctgcgtcat
     1081 atttacctca cgattcaggt aatcgtgctt gcgatcgctg gcggcctcga acgcgaagta
     1141 ttcggcatct catatggccc attgcgcccc gggactgtgt gagacgggca gcactgtgca
     1201 gcgggagtcg tcggaggatg gagcgtgagg cgcgcgggtt cgggtagtgt cggggccgat
     1261 gacgcgctgg atgttgcttg tcgcggcggc gtgcgggcac ccggggaacg gtgccaggtc
     1321 tgccgttgcg ccgcccgcgg atccggggca gccgacgcgc gacgccgaga tcgcggcgcg
     1381 cgcggcgccg ttcgtcgacg cgtacagcaa ctacggcgcg gtgctggcgc ccgacgggca
     1441 ggtggtgttc gtgtcgaacc gcgacgggct gccgcagctg tacgtcggcg cgctcggcga
     1501 gccggatcgg ccgccgcgcc ggctgccggt gccgcgcgag cgcgtggccg caccgcggct
     1561 gttgcccgac ggcaagacgc tggtgttcct gtccgacgtc ggttcggacg agaagtatca
     1621 cgtgtaccgg atcgggctcg atggcagcgg gctgaccgac ctgacgccgg acggcgatct
     1681 gcgccgcatg acgccgcaga tcgcgcgacg gtcgggcgcg atgctgtacg ccgcgcacgt
     1741 gctcgacgat caggcgacgc gggtgttcgc gcacacggtc gacgacccgc cgcgcgagat
     1801 ctaccgcgat cccaaggtcg ggttcatcgc cgacgtgtcg gccgatggcg cgcgcgcgc
//
LOCUS       DASVOR010000358         2891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_500251,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000358 DASVOR010000000
VERSION     DASVOR010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2891
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500251"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VF516_07375"
     CDS             complement(<1..194)
                     /locus_tag="VF516_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107533.1"
                     /translation="MDLTGTYEASTPVGVVTVMLHASREGRLQGEVRTGSIVLRLAGS
                     TDGVRGEGTATNELGANAVF"
     gene            complement(296..>2891)
                     /locus_tag="VF516_07380"
     CDS             complement(296..>2891)
                     /locus_tag="VF516_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8107534.1"
                     /translation="VIERDDGLMLCGERALLQGARTLLGRPTPCVGRDWELDALTAIL
                     DGGIDEPKVRAVMLIGAAGIGKSRLGAEFVSRARERHGELVIWGGRGDSLRAGSTLDL
                     LAQALCGAIGIRGSELLAERWDKIRTRVAEHVPLADQERVSGFLGELVGAPFPDEGEG
                     GAALRAARRDAQLMSEQMYKAWLDFLRAETSAHPVLLLLEDLHWSDAGTVRFIGTALR
                     ELPDRSWMVLALARPELHEVFPRLWAERQVQEIRLNGLGRRASERLVRQVLGADVGPT
                     TIEGLTKQADGNALYLEELIRAVAEGKDKDAPFPGTVLAMVETRLARLPRDARRTLRV
                     ASVFGEVCWEGGVVALLEGAMGEKVSELLATLVEQEVLVARPESRFPGEREFAFRHAL
                     LREGAYATLTAADQGLVHRLAGEWLEQRGERDPMVLAGHFERGGQGARAAEFYLCAAE
                     QALHVLDLDAAIVRADLGLGCAPPVEQRLALLGIRCEVARRLQRLAINEAEELIHAAP
                     RGSVPWARGMLSYHLRALMERRVEDLGRSLALLQDVTPTPGAAGAMSLIFLTRVFILD
                     TLGQLSQSTALEEPFRALVRAWAEQEPFVSVWWHIALGLRSSHAYDDPWNGLQHGDAI
                     RPVFEAIGGQLTFVNMQLVRGLNQWYLGALASAAHTLEGIPAADTVIGMASSLRRFSL
                     SWLCADCGALDQACALASELAESGRARDSRQEEARGRWALAEVLRRRGDLAAAEREVG
                     VALGMTMPLEYPGVLATLGGLRLAQGRAAEALAAAEEAMACGAAMGGCGMFRGAFVRL
                     AHAEALHATGAHDAAIRAIAQARARLLAIADKIADPGYRASFLSHVPENARTLALADA
                     WLGDPDRG"
BASE COUNT          428 a         1120 c          960 g          383 t
ORIGIN      
        1 tgaaacaccg cgttggcgcc gagctcgttg gtcgccgtcc cttcgccccg gacaccgtcg
       61 gtggagccag cgaggcgcag cacgatcgat cccgtccgga cctcgccctg taaccggccc
      121 tcgcgcgagg catggagcat caccgtcacc acgccaaccg gcgtgctcgc ctcatacgtc
      181 cccgtcaggt ccatgccagc tcccccggcc ctcatcatgt gcgccagctg cgtcgaagct
      241 caagccctga ctgcaggcag ctgctgccgt acccgtggac gaccgtggtg cgtcatcacc
      301 cccggtcggg atcgcccagc cacgcgtccg ccagggccaa cgtccgggcg ttctcaggca
      361 cgtgcgagag gaagctcgcc ctgtagcctg gatcggcgat cttgtcggcg atggcaagga
      421 ggcgcgcccg cgcctgggcg atggcgcgga tggcggcatc gtgggcgccg gtggcgtgga
      481 gcgcctcggc gtgggcgagg cgcacgaacg cgccgcggaa catgccacac ccgcccatgg
      541 cagcgccgca cgccatggcc tcctcggcgg cggcgagcgc ctccgcagcg cggccctggg
      601 cgaggcggag tcccccgagc gtcgcgagga cccccggata ctcgagaggc atcgtcatgc
      661 cgagcgcgac cccgacctcg cgctcggccg ctgccagatc gcccctccgg cggagcacct
      721 ccgcgagcgc ccagcgacca cgcgcctcct cctgccggct gtcgcgcgcg cgaccggact
      781 cggcgagctc gctggccagg gcgcacgcct ggtcgagcgc accgcagtcg gcgcacagcc
      841 acgatagcga gaaccggcgc agcgagctcg ccatgccgat caccgtgtcc gccgccggaa
      901 tcccctccag cgtgtgcgcc gcggacgcga gcgcgccgag gtaccactgg ttcagtcctc
      961 gaaccagctg catgttcacg aaggtgagct gaccgccgat ggcctcgaag accggccgga
     1021 tcgcgtcgcc gtgctgcaag ccgttccatg ggtcgtcgta cgcatgggag cttctgaggc
     1081 cgagggcgat atgccaccac acgctcacga acggctcctg ctccgcccac gcgcggacga
     1141 gcgcacgaaa cggctcctcc agcgcggtgc tctgcgagag ctgtccgagg gtatcgagga
     1201 tgaagaccct ggtgaggaag atgagcgaca tcgcccccgc cgccccgggg gtcggggtga
     1261 catcctggag cagcgccagc gatctcccga gatcctcgac ccggcgctcc atcagcgcgc
     1321 gcaggtgata ggaaagcatc ccccgtgccc aggggacgga gcctcgcggc gccgcgtgga
     1381 tcagctcctc ggcctcgttg atcgcgagcc tctgcaagcg ccgggcaact tcgcagcgga
     1441 tgccgagcag ggcgagtcgc tgctccaccg gaggcgcgca gccaaggccg aggtccgcgc
     1501 gaacgatcgc agcgtcgagg tcgaggacgt gaagcgcctg ctccgccgcg cacaggtaaa
     1561 attcggcggc gcgggcgccc tgtcctccgc gctcgaagtg tccggccagg accatcggat
     1621 cgcgctctcc gcgctgctcg agccactccc cggcgaggcg gtgcacgagc ccctgatcgg
     1681 cggcggtcag ggtggcgtag gcgccctcgc ggagcagcgc gtggcggaac gcgaactcgc
     1741 gctcgccggg gaaccggctc tcgggccgcg cgaccagcac ctcttgctcg acgagcgttg
     1801 cgagcaactc gctgaccttc tcgcccatgg ccccctcgag cagcgcgacc acaccaccct
     1861 cccagcacac ctcgccgaac acgctggcga cacgtagcgt ccgccgcgca tcgcgcggga
     1921 gccgcgcgag ccgggtctcg accatggcca gcaccgtccc ggggaagggg gcgtccttgt
     1981 ccttgccctc ggccacggcg cggatcagct cctccaggta gagcgcgttg ccatcggcct
     2041 gtttcgtcag gccctcgatc gtagtcggcc ccacgtcagc gcccaggacc tgccgcacca
     2101 gccgctcgct cgccctgcgc ccgaggccgt tgagccggat ctcctgcacc tggcgctccg
     2161 cccacagccg cgggaagacc tcgtgtagct cgggccgcgc cagcgcaagc accatccacg
     2221 accggtcggg gagctctcgc agcgccgtgc cgatgaaccg cacggtcccg gcatcgctcc
     2281 agtgcaggtc ctcgagcagg agcaggacag gatgcgcgga ggtctcggcc cggaggaagt
     2341 ccagccatgc cttgtacatc tgctcgctca tgagctgggc gtcccgtcgc gccgcccgga
     2401 gcgcggcgcc gccctcgccc tcgtcgggga acggcgcgcc gacaagctcc cccaggaacc
     2461 cgctcacccg ctcctgatcg gcgaggggca cgtgctcggc gacccgggtc cggatcttgt
     2521 cccatcgctc ggcgagcagc tcgcttcccc ggatcccgat ggccccgcac agcgcctggg
     2581 cgagcagatc gagcgtcgag cccgcgcgca gggagtcacc gcgtccgccc cagatcacga
     2641 gctcgccgtg acgctcccgg gcgcggctca cgaactcggc gccgagccgc gacttgccga
     2701 tccccgctgc tccgatcagc atcacggctc gcaccttcgg ctcgtcgatc cccccatcga
     2761 ggatcgcggt cagcgcgtcg agctcccagt cgcgcccgac gcacggcgtc ggccggccca
     2821 agagcgtcct cgctccctgg agcagcgcgc gctcgccgca cagcatcaac ccgtcatcgc
     2881 gctcgatcac c
//
LOCUS       DASVOR010000359         1891 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_558757,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000359 DASVOR010000000
VERSION     DASVOR010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_558757"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF516_07385"
     CDS             complement(<1..387)
                     /locus_tag="VF516_07385"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HEX8107535.1"
                     /translation="MRPVVTTAEMRALDRATIDDVGLPAVTLMETAGRAVAEAALRML
                     GSERGHVAVVCGPGNNGGDGFVAARVLRDRGIDAVVYLAAPRDAVVGDARVHLEILER
                     AGGIVRLLATPEQLAALDARVIDAALV"
     gene            complement(688..>1891)
                     /gene="glmM"
                     /locus_tag="VF516_07390"
     CDS             complement(688..>1891)
                     /gene="glmM"
                     /locus_tag="VF516_07390"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEX8107536.1"
                     /translation="GYMFESALASGIVAMGADVWLTGPLPTPGIAFITASMRCDAGVV
                     ISASHNPFEDNGIKLFARDGYKLPDHIEAEIEALMGSPELDGQRAAPADVGYSRKIED
                     ARGRYVVYCKATFPTELTLDGIHVVVDGAHGAAYRVGPAVFEELGARVTAIHTKPDGK
                     NINAAAGALHPEAMCETVRRHEAHIGIALDGDADRVVLCDEHGNVVDGDAVMALCATR
                     MIRENTLAKSTLVATVMSNIGLERAIRSAGGSVVRTQVGDRYVVEEMRKHGYNLGGEQ
                     SGHLVFLDHATTGDGIVAALRVLAIMAREGRPLSELARVMTRTPQVLINTAVERKIPL
                     DQLPDVQRMIAEIERQLGEDGRVLVRYSGTESKARVMIEGIDETRIRGWAEEIAGALA
                     KGCRAATT"
BASE COUNT          237 a          719 c          650 g          285 t
ORIGIN      
        1 caccagcgcg gcgtcgatca cccgggcgtc gagcgccgcc agctgctcgg gcgtcgccag
       61 cagccggacg atgccgccgg cgcgctccag gatctcgagg tggacgcggg cgtcgccgac
      121 caccgcgtcg cgcggcgcgg ccaggtacac caccgcgtcg atcccgcggt cgcgcagcac
      181 ccgggcggcc acgaacccgt cgccgccgtt gttgcccggg ccgcacacca cggccacgtg
      241 cccgcgctcg ctgcccagca tgcgcagcgc cgcctcggcg accgcgcgcc ccgcggtctc
      301 catcagcgtc accgcgggca ggccgacatc gtcgatcgtc gcgcgatcga gggcgcgcat
      361 ctccgccgtg gttaccaccg gtctcatcgg ccacagccta gcgccattcg cggcgccggc
      421 cgtgcccgcg gtgcgtcgat cgagaggggg ctttcccgtc ccctctgtcg cggcaaagcc
      481 gcgacattca cccctccgcg cgtcgcgtcg cgcggccctg cacctgtcag cacttgcctg
      541 cgcgccgtcg ccacgtcgcc gctcgatgtt cgcgcgcaat cctcttttgc cgagcccgat
      601 gacctactcg gccgactcat cgatcgagag ggctcatcgc gtcacgccaa tcaccgcggc
      661 catgcgccgg cgcacggtgg gaacacgcta cgtcgtcgct gcccggcagc ccttggccag
      721 cgctcccgcg atctcctcgg cccagccgcg aatgcgggtc tcgtcgatgc cctcgatcat
      781 cacgcgcgcc ttgctctcgg tgccgctgta gcgcaccagc acgcggccat cctcgccgag
      841 ctggcgctcg atctcggcga tcatccgctg cacgtcgggc agctggtcga gcgggatctt
      901 ccgctcgacc gcggtgttga tcaggacctg cggggtccgg gtcatcaccc gggcgagctc
      961 ggacagcggc cggccctcgc gcgccatgat cgcgagcacg cggagcgcgg cgacgatgcc
     1021 gtcgccggtg gtcgcgtggt cgaggaacac caggtggccg gactgctcgc cgccgaggtt
     1081 gtagccgtgc ttgcgcatct cctcgacgac gtagcgatcg ccgacctggg tgcgaaccac
     1141 gctgccgccg gcgctgcgga tcgcgcgctc gaggccgatg ttggacatca ccgtcgcgac
     1201 cagcgtcgac ttggccagcg tgttctcccg gatcatccgg gtcgcgcaca gcgccatcac
     1261 cgcgtcgccg tcgaccacgt tgccgtgctc gtcgcacagc accacgcggt cggcgtcgcc
     1321 gtcgagcgcg atcccgatgt gggcctcgtg gcggcgcacg gtctcgcaca tcgcctcggg
     1381 gtgcagcgcg ccggccgcgg cgttgatgtt cttgccgtcg ggcttggtgt ggatcgcggt
     1441 gacccgggcg ccgagctcct cgaacaccgc cgggccgacc cggtaggccg cgccgtgcgc
     1501 gccgtcgacc acgacgtgga tgccgtcgag cgtgagctcg gtggggaagg tcgccttgca
     1561 gtacacgacg tagcggccgc gcgcgtcctc gatcttgcgg ctgtagccga cgtcggcggg
     1621 cgcggcgcgc tggccgtcga gctcggggga gcccatcagc gcctcgatct cggcctcgat
     1681 gtggtccggc agcttgtagc cgtcgcgcgc gaacagcttg atcccgttgt cctcgaacgg
     1741 gttgtgcgac gccgagatca cgacgccggc gtcgcaccgc atcgacgcgg tgatgaacgc
     1801 gatgcccggg gtcggcagcg gcccggtgag ccagacgtcg gcgcccatcg ccacgatgcc
     1861 ggacgccagc gcggactcga acatgtagcc g
//
LOCUS       DASVOR010000360         4563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_324772,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000360 DASVOR010000000
VERSION     DASVOR010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4563
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324772"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VF516_07395"
     CDS             complement(<1..874)
                     /locus_tag="VF516_07395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107537.1"
                     /translation="MIASGQSIGNYRILNKIGTGGMGAVYLAEHPLIGKRVALKVIHR
                     ELAGNREVVSRFFQEARAVNKIGNEHIVEIHDFGVTPEGDHFYIMEYLEGRTLASVLA
                     REKALEVMRSLHIGAQIANALAAAHAAGIIHRDLKPDNIMLMARLGEQDFVKVLDFGL
                     AKVFSAENAVKTAAGVLLGTPQYMSPEACESKRDIDHRTDIYALGVLLFQMMTGALPF
                     EGETMGEVLVKQVTMLPPAPRAMNPAIPPSVEQILLRCLAKPVDARFPTMLQLREALL
                     DPEAYLQASPPIARA"
     gene            complement(935..2110)
                     /gene="moeB"
                     /locus_tag="VF516_07400"
     CDS             complement(935..2110)
                     /gene="moeB"
                     /locus_tag="VF516_07400"
                     /inference="COORDINATES: protein motif:HMM:NF006444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEX8107538.1"
                     /translation="MVTFDTLLRELRGKTPEIEPTTLDAALRGAARPALIDVREPDEH
                     AQGMIPGTLHIPRGFLELRIERNVPDRGTPVVIYCASGTRSVLAARSLGELGYTNVRS
                     LAGGFTGWKRAGLPWEQPISLRPDQESRYSRHTLLPEVGVAGQIKLLGAKALCIGAGG
                     LGSPSSMYLAAAGIGTIGMIDDDVVDASNLQRQILHGTDRVGVAKVESAETTLRGLNP
                     DVKVDKHRTRITSDNALALIAPYDVIIDGADNFATRYLVNDAALRLGKPVIHASIFRF
                     EGQITVFPAHGSPCYRCLYPQPPPAEEAPSCAEAGVLGVLPGIMGVLQATEAIKLILG
                     LGDTLAGRLLCYDAIKTRFRELRLRRDPACPTCGDGVDRSKIPLIDYEQFCAGPARH"
     gene            2137..2580
                     /gene="erpA"
                     /locus_tag="VF516_07405"
     CDS             2137..2580
                     /gene="erpA"
                     /locus_tag="VF516_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEX8107539.1"
                     /translation="MRVRCLVPGDFQELSKMSDLASAITPAIDNVPPEAPGPESMVLI
                     TSVAASKVNEIRDAEAIEAHMALRLRVVGGGCAGFSYDLYFDEPTEVDRQFVVQDVKV
                     VVDEMSLMYLVGTEIDYVEGLQGAGFKFNNPNVKTTCGCGSSFSV"
     gene            2631..4040
                     /locus_tag="VF516_07410"
     CDS             2631..4040
                     /locus_tag="VF516_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8107540.1"
                     /translation="MHDIVGVLQRELGSAAVIGPGHERLDDYGRDESPLPDFWPPDCA
                     VLVESTEQAAAVLRICLERKVPVTPRGAGSGMCGGALPIHGGVVLSTERMTRIKEIDA
                     GDLICVTEPGVVTGRLQEAVEAQQLFYPPDPASLEFCSIGGNASTNAGGLRAFKYGVT
                     REYILGLEVALMGGEVLRCGRRTSKGVTGYDLVAGFVGTEGTFGVITELTLKLVPLPM
                     AAAAVLGVFADVATAGAAISALLRGGLRPRVLELADRASIAAIRDKSRYRFPASAGAI
                     VLLEVDGEPEAMPMMMEKIGLRCDELGALDVLAATEPAERRAVWEARRLISPALKAAY
                     RIKLNEDLCVPRSRIVEMLARVDRVAADTGVECAVFGHAGDGNLHVNLLSNDDPADPA
                     VRKHLWGAARQLFAHTVELGGTLSGEHGVGITKRDYVAMEYSEQVLEWQRRWKAMWDP
                     SGLLNPGKRLPPRPRGCSE"
     gene            4244..>4563
                     /locus_tag="VF516_07415"
     CDS             4244..>4563
                     /locus_tag="VF516_07415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107541.1"
                     /translation="MIALVRDRPAFAASLLRDVLSVEVPQFTEARLTEATLNQLVPVE
                     YHADAVVLFIDLVDHENKPVFGTICEVQLQPDARKLFTWPLYSVAARARHKCPFVVTV
                     VTADP"
BASE COUNT          653 a         1618 c         1581 g          711 t
ORIGIN      
        1 gcgcccgcgc gatcggcggc gatgcctgca gatacgcttc gggatcgagc agcgcctcgc
       61 gcagctgcag catcgtcggg aaccgcgcgt cgaccggctt ggccaggcag cgcagcagga
      121 tctgctcgac gctcggcggg atcgccgggt tcatcgcgcg cggcgccggc ggcagcatcg
      181 tgacctgctt gaccagcacc tcgcccatgg tctcgccctc gaacggcagc gccccggtca
      241 tcatctggaa cagcagcacg ccgagcgcgt agatgtcggt gcggtgatcg atgtcgcgct
      301 tgctctcgca cgcctccggc gacatgtact gcggcgtgcc gagcagcacc ccggcggccg
      361 tcttcaccgc gttctccgcc gagaacacct tcgccagccc gaagtcgagc accttgacga
      421 agtcctgctc gcccagccgc gccatcagca tgatgttgtc gggcttgaga tcgcggtgga
      481 tgatcccggc ggcgtgcgcc gcggcgagcg cgttggcgat ctgcgctccg atgtgcagcg
      541 accgcatcac ctcgagcgcc ttctcgcgcg ccagcaccga cgccagcgtc cggccctcga
      601 ggtactccat gatgtagaag tgatcgccct cgggcgtcac cccgaagtca tggatctcga
      661 cgatgtgctc gttgccgatc ttgttgaccg cgcgcgcctc ctggaagaac cgcgacacca
      721 cctcgcggtt gccggcgagc tcgcggtgga tcaccttgag cgcgacccgc ttgccgatca
      781 gcgggtgctc ggccaggtac accgcaccca tcccacccgt cccgatcttg ttcaggatcc
      841 gatagttccc gatcgactga ccggacgcga tcacacgttc ggcaagtgta tcagctgcgt
      901 gcgccgcctg cccatcagcg tctcgacggc gccctcagtg ccgggccggg ccggcgcaga
      961 actgctcgta gtcgatcagc gggatcttgc tgcggtcgac gccgtcgccg caggtcgggc
     1021 acgccggatc gcggcgcagc cggagctcgc ggaaccgggt cttgatcgcg tcgtagcaca
     1081 gcagccggcc ggcgagcgtg tcgcccagcc cgaggatcag cttgatcgcc tcggtcgcct
     1141 gcaggacgcc catgatgccg ggcagcacgc cgagcacgcc ggcctcggcg cacgacggcg
     1201 cctcctcggc aggcggcggc tgcgggtaga ggcagcggta gcacggcgag ccgtgcgccg
     1261 ggaacacggt gatctggccc tcgaaccgga agatcgaggc gtggatcacc ggcttgccca
     1321 gccggagcgc ggcgtcgttg accaggtagc gggtcgcgaa gttgtcggcg ccgtcgatga
     1381 tgacgtcgta cggcgcgatc agcgcgagcg cgttgtccga ggtgatccgg gtccggtgct
     1441 tgtcgacctt gacgtcgggg ttgagcccgc gcagcgtggt ctcggcgctc tcgaccttgg
     1501 cgacgccgac gcggtcggtg ccgtgcagga tctggcgctg caggttggac gcgtcgacca
     1561 cgtcgtcgtc gatcatgccg atcgtgccga tcccggcggc ggcgaggtac atgctcgacg
     1621 gcgagcccag cccgccggcg ccgatgcaca gcgccttggc gccgagcagc ttgatctgcc
     1681 cggccacgcc gacctcgggg agcagggtgt gccgcgagta gcggctctcc tgatcggggc
     1741 gcagcgagat cggctgctcc cagggcagcc cggcgcgctt ccagccggtg aacccgccgg
     1801 cgagcgagcg gacgttggtg tagccgagct cgccgaggct gcgcgccgcg agcaccgagc
     1861 gcgtgccgct cgcgcagtag atcacgaccg gcgtgccgcg gtcgggcacg ttgcgctcga
     1921 tgcgcagctc gaggaagccg cgcgggatgt gcagcgtgcc ggggatcatg ccctgggcgt
     1981 gctcgtccgg ctcgcgcacg tcgatcagcg ccgggcgtgc ggcgccgcgc agcgcggcat
     2041 cgagcgtggt cggttcgatc tcgggggtct ttccgcgaag ctcgcggagc agcgtgtcaa
     2101 aggtcaccac ggcgggtaca ttgccatgac cctcacgtga gggtaaggtg tctcgtcccc
     2161 ggagatttcc aggagctctc gaagatgtcc gatcttgcca gtgcgatcac ccccgccatc
     2221 gacaatgtgc cacccgaggc ccccggcccc gagtcgatgg tgttgatcac gtctgtggcc
     2281 gcctccaagg tcaacgagat ccgcgacgcc gaggcgatcg aggctcacat ggcgcttcgg
     2341 ctgcgcgtgg tcggcggcgg ctgcgcgggc ttctcgtacg acctgtactt cgacgagccg
     2401 accgaggtcg accgccagtt cgtggtccag gacgtcaagg tcgtcgtcga cgagatgagc
     2461 ctgatgtacc tggtcggcac cgagatcgac tatgtcgagg gcctgcaggg ggcgggcttc
     2521 aagttcaaca accccaacgt caagaccacc tgcggctgcg gctcgtcgtt cagcgtgtga
     2581 cgatctcggc cccggcccgc gccgggcgac gaaggcgcta ggctgtcgcc gtgcatgaca
     2641 tcgtaggcgt gctccagcgc gagctcgggt ccgcggccgt gatcggcccc ggccacgagc
     2701 gcctcgacga ctacggccgc gacgagagcc cgctgcccga cttctggccg ccggactgcg
     2761 cggtgctcgt cgagtcgacc gagcaggccg ccgcggtgct gcggatctgc ctcgagcgca
     2821 aggtcccggt caccccgcgc ggcgcgggca gcgggatgtg cggcggcgcg ctgccgatcc
     2881 acggcggcgt cgtgctgtcg accgagcgga tgacgcggat caaggagatc gacgccggcg
     2941 atctgatctg cgtgaccgag cccggcgtgg tcaccggccg gctgcaggag gcggtcgagg
     3001 cccagcagct gttctacccg cccgaccccg cgtcgctcga gttctgctcg atcggcggca
     3061 acgcctcgac caacgccggc ggcctgcgcg ccttcaagta cggcgtcacc cgcgagtaca
     3121 tcctcggcct cgaggtcgcg ctgatgggcg gcgaggtgct gcgctgcggc cggcgcacct
     3181 ccaagggcgt caccggctac gacctggtcg ccggcttcgt cggcaccgag ggcacgttcg
     3241 gcgtgatcac cgagctcacc ctcaagctcg tcccgctgcc gatggccgcc gccgccgtgc
     3301 tcggcgtgtt cgccgacgtc gccaccgccg gcgccgcgat ctcggcgctg ctgcgcggcg
     3361 gcctgcgccc gcgcgtgctc gagctcgccg atcgcgcgtc gatcgccgcg atccgcgaca
     3421 agagccggta ccgcttcccc gccagcgccg gcgcgatcgt cctgctcgag gtcgacggcg
     3481 agcccgaggc gatgccgatg atgatggaga agatcggcct gcgctgcgac gagctcggcg
     3541 cgctcgacgt gctggccgcg accgagccgg ccgagcgccg cgcggtgtgg gaggcgcgcc
     3601 ggctgatctc gccggcgctc aaggccgcct accggatcaa gctcaacgag gacctgtgcg
     3661 taccgcgcag ccggatcgtc gagatgctgg cccgggtcga ccgcgtcgcc gccgacaccg
     3721 gcgtcgagtg cgccgtgttc ggccacgccg gcgacggcaa cctccacgtc aacctgctgt
     3781 ccaacgacga ccccgccgac cccgccgtgc gcaagcacct gtggggcgcc gcccgccagc
     3841 tgttcgccca caccgtcgag ctcggcggca cgctgtccgg cgagcacggc gtcggcatca
     3901 ccaagcgcga ctacgtcgcg atggagtact ccgagcaggt gctcgagtgg cagcggcggt
     3961 ggaaggcgat gtgggatcca tcgggcctgc tcaaccccgg caagcgcctc ccaccgcggc
     4021 cccgcggctg cagcgagtga ccaccgcggc gtcgagttgc agctcgagac ccacgggatg
     4081 gcgcgacgct gtccgcgttc gcagcgtcgg tccacgattg acaatcgacg ctcatcaaaa
     4141 gagctcgcca ttcccttcga agcgcggcat tccccttttc gaggatccga tgtcctgcat
     4201 agagtcgcgg tatctcatgc cgccgtctct gaatcacgag ggcgtgatcg cgttggtccg
     4261 ggaccggccg gcatttgccg ccagcttgct ccgcgacgtg ttgagcgtcg aggttccgca
     4321 gttcaccgag gcacggctca cggaagcgac gctcaatcag ctcgtgccgg tcgagtacca
     4381 cgccgacgcg gtcgtgctgt tcatcgatct cgtggaccac gagaacaagc ctgtgttcgg
     4441 gaccatctgc gaggtccagc tccagcccga cgcgcgcaag ctgttcacct ggccgctcta
     4501 ttccgtcgcc gcgcgcgccc gccacaagtg tccgttcgtc gtgacggtcg tgacggccga
     4561 ccc
//
LOCUS       DASVOR010000361         1694 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_61842,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000361 DASVOR010000000
VERSION     DASVOR010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1694
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61842"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..933)
                     /locus_tag="VF516_07420"
     CDS             complement(127..933)
                     /locus_tag="VF516_07420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8107542.1"
                     /translation="MPARLYHERIARSDTAPVRWLALTHGIYGAGSNWRSVARKLTER
                     RPEWGFVLVDLRQHGRSEPGAPPHTIAACADDLHAQFAELGGVAAVAGHSFGGKVALA
                     TRPLGALRQTWVLDASPSARPGAAVDPANTVMQVLELMERAPRGWARRDDFVASVVQA
                     GHDEGLARWLAMSLAPEPGGTLALRFDFAALREMLASYYATDLWDALEAPGGDVEVVV
                     AERSPAVSAADRGRLAAAPPHVHVHPVAAGHWLHIEAPAAVVDLLASRLP"
     gene            959..1597
                     /locus_tag="VF516_07425"
     CDS             959..1597
                     /locus_tag="VF516_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107543.1"
                     /translation="MDALAALESLASAGFDLAHTFDAAAAAREPGLAVLAGPERLGIL
                     IGNTRALWPRFTDALRDPALAAERDPLDRYTERAIDAAFPGAAFPGARIYYAHRQYDG
                     AFVPLQHLAVATGLGALAPSRLVIHPIYGPWFALRAALVVAGEPPARRPIPKPCTCDA
                     ACTTVLARAQRSTDWRDWVALRDTCSLRTWRYSDDQVRYHYATVWGLRRQEV"
BASE COUNT          209 a          660 c          602 g          223 t
ORIGIN      
        1 ggcgtcgccc ggctcggcgc ggaccgccgg cgcgccgacc cccagcgcca ccgcacacgc
       61 gaccacagcg cggaccatca ccgctggaca cctcccacgc cccgcggttg catcatgccc
      121 acgcgatcat ggtagccgcg atgcgagcag atcgaccacc gcggccggcg cctcgatgtg
      181 caaccagtgc ccggcggcca ccgggtggac gtggacatgc ggcggcgcgg cggccagccg
      241 gccgcgatcg gcggcgctca ccgcgggcga gcgttcggcc acgacgacct cgacgtcgcc
      301 gccgggagcc tccagcgcgt cccacagatc ggtggcgtag taggacgcca gcatctcgcg
      361 cagcgccgcg aagtcgaacc gcagcgccag cgtgcccccc ggctcgggcg caaggctcat
      421 cgcgagccag cgcgccaggc cctcgtcgtg gccggcctgg accaccgagg cgacgaagtc
      481 gtcgcgccgg gcccagccgc gcggcgcgcg ctccatgagc tccagcacct gcatcaccgt
      541 gttcgcagga tcaacggccg cgccgggccg cgcgctcggg ctggcgtcga gcacccaggt
      601 ctggcgcagc gcacccagcg gccgggtcgc gagcgcgacc ttgcccccga agctgtggcc
      661 cgcgaccgcg gcgacgccgc cgagctcggc gaactgcgcg tgcaggtcgt cggcgcacgc
      721 ggcgatcgtg tgcggcggcg cgccgggctc cgagcgaccg tgctggcgca ggtcgaccag
      781 cacgaagccc cactccggcc gccgctcggt cagcttgcgc gcgacgctgc gccagttgct
      841 gcccgcgccg tagatcccgt gggtcagcgc gagccagcgc accggcgcgg tgtcgctccg
      901 ggcgatgcgc tcgtggtaca gtcgcgcggg cacgcccgga gtgtgccatg gtcgccgtgt
      961 ggatgcgctc gctgcgctcg agtcactcgc ctcggccggg ttcgatctcg cccacacgtt
     1021 cgacgccgcg gccgccgcgc gcgagcccgg cctcgcggtg ctcgccggcc ccgagcggct
     1081 cggcatcctg atcggcaaca cccgcgcgct gtggccgcgg ttcaccgacg cgctgcgcga
     1141 tccggcgctc gccgcggagc gcgatccgct cgaccgctac accgagcgcg cgatcgatgc
     1201 ggcatttccc ggggcggcgt ttcccggggc gcggatctac tacgcccacc gccagtacga
     1261 cggcgcgttc gtccccctcc agcacctcgc cgtcgccacc gggctcggag cgctcgcgcc
     1321 gagccgcctg gtgatccatc cgatctatgg cccgtggttc gcgctgcgcg ccgcgctggt
     1381 ggtcgccggg gagcctcccg cgcgccggcc gatcccgaag ccgtgcacgt gcgacgcggc
     1441 gtgcacaact gtgctggcgc gtgcacagcg atccaccgac tggcgggact gggttgcact
     1501 tcgcgatacc tgcagcttgc gaacctggcg gtacagcgac gatcaggtcc gctatcacta
     1561 cgcgaccgtc tggggcctcc ggcgtcagga ggtttgagcg aagtcgcgcg cgcggcgtgg
     1621 gaagctcggc ccatgcgcta cgcatgcctc gtgtggttga tcgcgggttg tgggttctcc
     1681 tctccggcca gctc
//
LOCUS       DASVOR010000362         6116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_178861,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000362 DASVOR010000000
VERSION     DASVOR010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178861"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            481..1869
                     /locus_tag="VF516_07430"
     CDS             481..1869
                     /locus_tag="VF516_07430"
                     /inference="COORDINATES: protein motif:HMM:NF024140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8107544.1"
                     /translation="MQQWKDERHTRRMLRAAFGGIAALLLVSQILGYFRWKAAQDTVA
                     EIEGDALTSVRLIGRMSYDFQRERILIDRHIFEHDAAQMAAIERQIAAVKDDGAAVAR
                     AYAPLTNYDGEAAAWFDLNRDVAISQRQAAAALELSRGNHDSEAAQVMIAAEPAFEAV
                     SRGVQRLVDVNQASAAAARVHAATLEADVLYVLLALAAAILLITLVVGRQLTWLISDN
                     ERRLRQYMVELQDKNRELDAFAGRVAHDLRGPLHTIHLAASLIAERVPQESARTAILQ
                     RGVTQMTGLVDDLLALSRAGGPTDGAVARPEAVAASIEADLRPRVSEAGGTLRVDVQP
                     AAIRCSDGLLRQALWNLGENAVKYRRPDVPPVIAVVGRATSTGYELRVSDNGVGMSDE
                     DTRRAFEPFFRARRTQAITGTGLGLAIVRRIVEASGGTVAADSHPDHGTAITLRFLLA
                     PVPDGASLPGAA"
     gene            complement(1908..3266)
                     /locus_tag="VF516_07435"
     CDS             complement(1908..3266)
                     /locus_tag="VF516_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8107545.1"
                     /translation="MTCVFVVDDEREMVDLIALGLKKRGFQIIPFGNGADALTAISNH
                     DVDVIVTDLNMKGMTGLELCQRVVADRPDIPVLMLTAFGSFETAVGAIRAGAYDFVTK
                     PVEIEALAIAVRRAAEHRALRGEVKRLREVVASTRRHGELVGASPAMQQIYQLIDQVS
                     ATDATVLITGESGTGKEVVAREIHNRSRRKAAPFVVVNCAAVPEALFESELFGHAKGA
                     FTDAKHSRQGLFQQANGGTLFLDEIGEMALALQPKLLRALQERKVRPVGAEAEISVDV
                     RLVTATNCELEDMVEDKRFREDMYYRINVIHIPLPPLRARGGDVLLLAQHLLRHYAAV
                     FDKKVMGLSAAAAERMMAYDWPGNVRELGNCLERAVALAHFEEIQVEDLPDKIRNSRR
                     ATAMSGGELTELLTLEEVERRHVLRVLEACHGNRTDAAKILGLDRKTLYRKLLRWGVG
                     DE"
     gene            complement(3263..4810)
                     /locus_tag="VF516_07440"
     CDS             complement(3263..4810)
                     /locus_tag="VF516_07440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8107546.1"
                     /translation="MRLTRKFIAALVIGVALVALVQNYLDYQREREVFDRQMQGEADA
                     LGRALGLGLADVWHRDGEAAAREFLIDANNANKSGRLRVRWVWLDTKGHDAPLEPRDQ
                     LEPMKQDSVVVIRDRARRRLVTYIPVKVPEPRDGGLEIQQSLEPFDTYVRDSLRNQII
                     GSAAAVLIAAVLALALGVVFIGRPISKLASKARRVGTGDLSDPLRLRQRDELGELANE
                     INLMCDRLAEEQAARARATEQLRHADRLTTVGKLASGLAHELGTPLNVVSGRARLIAD
                     HEVEGNDVVDSARIVAEQADRMTALIRQLLDFARPRAPRPAPLNVTTLASRVCQLVAT
                     IARKANVKLIPPAPDESLRLEADDGQLTQVLTNLVVNAIQSIGDDGTVEIVTRTVDQV
                     PPPYVGGAVQTWMAIEVRDTGAGMDDATRERIFEPFFTTKQVGEGTGLGLSVSWGIVR
                     EHGGWIDVQSSRNKGSTFTVYLPMTRRVGRTTGERNALVNERGAERGAERGTERGTER
                     GSDRGAA"
     gene            5046..5513
                     /locus_tag="VF516_07445"
     CDS             5046..5513
                     /locus_tag="VF516_07445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8107547.1"
                     /translation="MLPKHILVPTDLSEGAEQALDYACELARTLDAELHLLNVVGIPA
                     LGVPELGVALASTVIDQLVVENQTALQELARTRCSTSRVGQILIKAGDPRDTINQTAR
                     DLGIDLIIMGTHGRRGITRALLGSVAETVVRTAPCAVLTVRLREASDSDRHAA"
     gene            5652..>6116
                     /locus_tag="VF516_07450"
     CDS             5652..>6116
                     /locus_tag="VF516_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEX8107548.1"
                     /translation="MIRALPTHESRRQRMVSEQLMRRDIVDPRVLAAFSSVPREQFVP
                     GDLAAQAYDDAPLPIGSGQTISQPYVVAVTVQALQLQGHERVLEIGTGSGYAAAILGT
                     LAREVTTLERIAELAAAAADRLARLGFSSIDVQHGDGSLGWPPGAPYEAIAVA"
BASE COUNT          888 a         2169 c         2164 g          895 t
ORIGIN      
        1 ccagcgccat gccaaccagg ccgcgacggc ccggagcaac ttcaacgcgc tcagcgcgac
       61 caaccaggcc aacgtgctgg cgttcatcaa ctcgctgtag ccgcgtgcgc tgaccgacgg
      121 cgacgcgatc cccggcggtg agccgccgcc cgggcagcga cccggcgcgc tcgtcattcg
      181 tcgtagcccg aggcgcgatg gtacaccggc gcgcgacgag cgtcacgcca ccgtggcacg
      241 ccatcgttca cgcgaaccgt tcacgcgaac cgttcacgcg aaccatgtgc gcggacccgt
      301 gcacgcggac cggcgaccgc gtcacggtgg ggccatccgc cgcatcccga gcggcgccgg
      361 gggtcgagag accttgcgca agcgcgacgg cccgcgcacg cttggtcaca gcggccctgt
      421 gcgctgcggc cggcgacgag catcccggtc gccgcgagcg ccgccgccga gcaggttgcc
      481 atgcagcagt ggaaagacga gcgacacacc cggaggatgc tgcgggctgc gttcggaggg
      541 atcgcggcgc tgctgctcgt gtcgcagatc ctggggtact tccggtggaa ggcggcgcag
      601 gacacggtgg ccgagatcga gggcgacgcg ctgaccagcg tccgtctgat cggacggatg
      661 agctacgact tccagcgcga gcggatcctg atcgatcgcc acatcttcga gcacgatgcc
      721 gcacagatgg ccgcgatcga gcggcagatc gccgcggtca aggacgacgg ggccgcggta
      781 gcgcgcgcgt acgctccgct cacgaactac gatggcgagg cggcggcgtg gttcgacctg
      841 aaccgcgacg tggcgatctc ccagcggcag gccgcggcgg cgctcgagct gtcgcgcggc
      901 aaccacgaca gcgaggccgc gcaggtgatg atcgcggccg agccggcgtt cgaggcggtg
      961 tcgcgcggcg tccagaggct ggtcgatgtc aaccaggcct ccgccgccgc cgcgcgggtg
     1021 catgcggcca cgctggaggc cgacgtcctc tatgtcctgc tcgccctggc tgcggcgatc
     1081 ctgctgatca cgctggtggt cggccggcag ttgacctggc tcatctcgga caacgagcgg
     1141 cggctccggc agtacatggt cgagctccag gacaagaacc gcgagctcga cgcgttcgcc
     1201 ggccgggtcg cccatgacct gcgcggcccg ctccacacga tccacctggc ggcctcgctg
     1261 atcgccgagc gcgtcccgca ggagtccgcc aggacggcga tcctgcagcg cggcgtcacc
     1321 cagatgaccg gcctggtcga cgatctgctc gcgctgtccc gcgccggggg cccgaccgac
     1381 ggcgccgtcg ctcggcccga ggcagtcgcg gcctcgatcg aggccgatct ccgcccgagg
     1441 gtgagcgagg ccggcggaac gctgcgggtc gacgtccagc cggccgcgat ccggtgcagc
     1501 gacgggctgc tccgccaggc gctgtggaac ctgggcgaga acgccgtgaa gtatcgccgg
     1561 cccgacgtgc cgccggtgat cgcggtggtc ggccgcgcca cgagcacggg ctacgagctc
     1621 cgggtgtcgg acaacggcgt cgggatgtcg gacgaggaca cccgccgcgc gttcgagccg
     1681 ttctttcgcg ccaggcggac gcaggcgatc acggggaccg ggctcgggct cgcgatcgtc
     1741 cgccgcatcg tcgaggcgag cggcgggacg gtggcggccg actcccatcc cgatcacggg
     1801 accgcgatca cgctgcgctt tctgctcgcg ccggtccccg acggcgcaag cctgcccggc
     1861 gcggcgtgac ctgcgcccga tcgacagctc tcggtcgttg ccgcgcgcta ctcgtccccg
     1921 acgccccacc gcaacagctt gcggtagagc gtcttgcgat cgagccccag gatcttggcg
     1981 gcgtcggtgc ggttgccgtg acaggcctcc agcacgcgca gcacgtggcg gcgctcgacc
     2041 tcctcgagcg tgagcagttc ggtcagctcg ccgcccgaca tcgcggtggc acgacgagaa
     2101 ttacggatct tgtcgggtag gtcttccact tggatctcct cgaagtgagc cagcgccacg
     2161 gcacgctcca gacagttacc gagctcgcga acgttgccgg gccagtcgta cgccatcatc
     2221 ctctcggcgg cggccgccga gaggcccatc actttcttgt cgaacaccgc cgcgtagtga
     2281 cgcagcaggt gctgggcgag taacagcacg tcgccgccgc gggcgcgcag cggcggcaac
     2341 gggatgtgga tcacgttgat gcggtaatac atgtcctcgc ggaaccgctt gtcctcgacc
     2401 atgtcctcga gctcgcagtt ggtcgcggtg acgagccgga cgtcgaccga gatctcggcc
     2461 tcggcgccga ccgggcggac cttgcgctcc tgcagcgcgc gcaacagctt gggctgcagc
     2521 gcgagcgcca tctcgccgat ctcgtcgagg aacagcgtgc cgccgttggc ctgctggaac
     2581 agcccctgcc ggctgtgctt ggcgtcggtg aacgcgccct tggcgtggcc gaacagctcg
     2641 ctctcgaaca gcgcctcggg gaccgccgcg cagttgacca cgacgaacgg cgccgccttg
     2701 cgccgcgatc ggttgtggat ctcccgggcg acgacctcct tgccggtgcc gctctcgccg
     2761 gtgatcagca cggtggcgtc ggtcgcgctg acctggtcga tcagctggta gatctgctgc
     2821 atcgccgggc tggcgccgac cagctcgccg tggcgccggg tgctggcgac cacctcgcgc
     2881 agccgcttga cctcgccgcg caacgcgcgg tgctctgccg cccgccggac cgcgatcgcg
     2941 agcgcctcga tctcgaccgg cttggtgacg aagtcgtagg ccccggcgcg gatcgcgccg
     3001 accgcggtct cgaagctgcc gaacgcggtc agcatcagca ccgggatgtc ggggcggtcg
     3061 gcgaccacgc gctggcacag ctcgaggccg gtcatcccct tcatgttcag atcggtgacg
     3121 atcacgtcga cgtcgtggtt ggagatcgcc gtcagcgcgt cggcgccgtt gccgaacgga
     3181 atgatctgga acccgcgctt cttgaggccg agcgcgatca ggtcgaccat ctcgcgctcg
     3241 tcatcgacca cgaacacgca ggtcacgcgg cacctcgatc cgagcctcgt tccgtgcctc
     3301 gttccgtgcc tcgttccgcg cctcgttccg cgcctcgttc gttgaccagc gcgttgcgct
     3361 cgccggtggt gcgtccgacg cgccgggtca tcgggagata gaccgtgaag gtcgacccct
     3421 tgttacgtga agattgcaca tcgatccagc cgccgtgctc gcgcacgatg ccccagctga
     3481 ccgacaggcc cagcccggtg ccctcgccga cctgcttggt ggtgaagaac ggctcgaaga
     3541 tgcgttcgcg ggtcgcgtca tccatgccgg cgcccgtatc acgaacctcg atcgccatcc
     3601 atgtctggac ggcgccgccg acgtacggcg gggggacctg gtcgacggtg cgggtgacga
     3661 tctccaccgt gccgtcgtcg ccgatcgact ggatcgcgtt caccaccagg ttggtcagca
     3721 cctgggtgag ctggccgtcg tcggcctcca gccggagcga ctcgtcgggc gccggcggga
     3781 tcagcttgac gttggccttg cgcgcgatgg tcgcgaccag ctggcacacc cgcgacgcca
     3841 gcgtggtgac gttgagcggc gccggccggg gcgcgcgggg ccgggcgaag tcgagcagct
     3901 ggcggatcag cgcggtcatc cgatcggcct gctcggcgac gatccgcgcc gaatcgacga
     3961 cgtcgttgcc ctcgacctcg tggtcggcga tcagccgggc gcggccggac acgacgttga
     4021 gcggcgtgcc gagctcgtgg gccaggccgg acgcgagctt gccgacggtg gtgagccggt
     4081 cggcgtggcg cagctgctcg gtcgcgcgcg cgcgggcggc ctgctcctcg gcgagccggt
     4141 cgcacatcag gttgatctcg ttggcgagct cgccgagctc gtcgcgctgg cggagcctca
     4201 gcggatcgct gaggtcgccg gtcccgaccc tgcgcgcctt gctggccagc ttggagatcg
     4261 gccggccgat gaacacgacg ccgagcgcga gcgccaggac cgccgcgatc aggaccgcgg
     4321 cggccgagcc gatgatctgg ttgcgcagcg agtcgcggac gtaggtgtcg aacggctcca
     4381 gcgattgctg gatctccagc ccgccgtcgc gcggctcggg gaccttgacc gggatatagg
     4441 tgaccagccg gcgccgggcg cgatcgcgga tcaccacgac gctgtcctgc ttcatcggct
     4501 cgagctggtc gcgcggctcg agcggggcgt cgtgcccctt ggtgtcgagc cacacccacc
     4561 ggacgcgcaa ccgcccgctc ttgttggcgt tgttggcgtc gatcaggaac tcccgggccg
     4621 ccgcctcgcc gtcgcgatgc cagacgtcgg ccaggcccag accgagcgcg cgcccgagcg
     4681 cgtccgcctc gccctgcatc tggcgatcga acacctcacg ttcgcgctgg tagtcgagat
     4741 agttctggac cagggcgacg agcgcgacgc cgatcaccag cgccgctatg aacttgcgtg
     4801 tgagtcgcat gccgcacagc agttctaccc cggcgtccgc agccccgatc acgccggtcc
     4861 tttcacgcat ccgttccggg gcataatgcc ccgaaaacac agggccaaag tcccccgcga
     4921 gatcgagcgc ggtggtcgca gcgtgcgcga aaacgacgac gcagcgacat tcattgcgcg
     4981 gcgatcccac catttcggga tggcccgatt tgtgaagaag tgattaggaa acaatcaggt
     5041 acgccatgct cccgaaacac atcctggtcc ccacggatct cagcgaaggc gcggagcaag
     5101 cgctggatta tgcgtgtgag ctggcgcgga ccctggacgc cgagctgcac ctcctgaacg
     5161 tggtcggcat cccggcgctg ggtgtgcccg agctcggcgt tgcgctggcc agcacggtga
     5221 tcgaccagct ggtggtcgag aaccagaccg cgctgcagga gctcgcccgg acgcggtgca
     5281 gcacgtcccg ggtcgggcag atcctgatca aggccggcga cccgcgcgac acgatcaatc
     5341 agaccgcgcg ggacctcggc atcgacctga tcatcatggg gacccacggc cgccggggga
     5401 tcacccgcgc gctgctcggc agcgtcgccg agaccgtcgt gcgcacggcc ccgtgcgccg
     5461 tgctgaccgt gcggctgcgc gaggcgagcg acagcgaccg ccacgcggca tgaccgcggg
     5521 ggggcgcttc cgcacgcgcg ctcgccgacg accgtgcgcc ggcgctgcgt acgacgcagc
     5581 gcagcgtgag atcgggccgg tgatgacgtg cgagcgttgc ccgggtctgg gaacgagcgc
     5641 atggtgagca catgatcagg gcgctcccga cgcacgagtc ccggcggcag cgcatggtca
     5701 gcgagcagct gatgcgccgc gacatcgtcg acccgcgcgt gctggccgcg ttcagcagcg
     5761 tgccgcgcga gcagttcgtg ccgggcgacc tcgccgcgca ggcctacgac gacgcgccgc
     5821 tgccgatcgg cagcggacag acgatctcgc agccctacgt cgtcgcggtg accgtgcagg
     5881 cgctccagct ccagggccac gagcgcgtgc tggagatcgg caccggctcg ggctacgccg
     5941 cggcgatcct cggcacgctg gcgcgcgagg tgacgaccct cgagcggatc gcggagctcg
     6001 cggcggccgc cgccgaccgc ctggcgcggc tcggcttctc cagcatcgac gtccagcacg
     6061 gcgacggctc gctgggctgg ccgccgggcg cgccgtacga ggcgatcgcc gtcgcg
//
LOCUS       DASVOR010000363         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266208,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000363 DASVOR010000000
VERSION     DASVOR010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266208"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF516_07455"
     CDS             complement(<1..407)
                     /locus_tag="VF516_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8107549.1"
                     /translation="MAKELREYERKRDFQDTPEPAPQHPRPAAGAPRFVIQEHSATRL
                     HWDLRLEHDGALASWAIPNGIPYDPKENRKAVHTEDHPLEYLTWEGEIPKGEYGAGTM
                     RVWDSGTYELEKWTEAKVMVEFHGERLRGRYAL"
     gene            453..1406
                     /gene="gluQRS"
                     /locus_tag="VF516_07460"
     CDS             453..1406
                     /gene="gluQRS"
                     /locus_tag="VF516_07460"
                     /inference="COORDINATES: protein motif:HMM:NF004315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEX8107550.1"
                     /translation="MKTGRFAPSPTGDLHLGNLRTALLAWLFARSAGGAFVLRVEDLD
                     RPRVRPGAGAQMLADLHWLGLDWDEGPDVGGPLGPYTQSIRQALYAGALARLRDRDLL
                     YPCYCTRAELQALAGIASAPQGGDDTPRYPGTCRDLSERQRRQREREGRRPALRFRVP
                     DAEIAFDDHIAGPRREQVAASVGDFVVRRSDGIVAYQLAVVVDDALMGITQVVRGADL
                     LTSTARQLALFDALGYARPQSFAHVPLALDPNGVRMAKRDASSGIGEARSSGLDHRVV
                     LGALAASAGIWPPGETATPDQLVAAFDPRLITSTPTPVSLV"
     gene            complement(1443..>1994)
                     /locus_tag="VF516_07465"
     CDS             complement(1443..>1994)
                     /locus_tag="VF516_07465"
                     /inference="COORDINATES: protein motif:HMM:NF025135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4166 domain-containing protein"
                     /protein_id="HEX8107551.1"
                     /translation="IWHGAFYTLPFLYIGSWRRIMFPEHGRNVPFTIENYAFVDDVGR
                     ETVTWVRTFKTRKTRRFDAYMSYNQRRGRIVDYLGSHEHLAVDLDLAVDERGGLRLRS
                     GAQRLYEGVVGFSFPLFFSGIADVCEWYDDTDQRFHIDVNVHNRTWGPLFGYRGAFDV
                     EWRPVEPNTLPARLRPMRTERRD"
BASE COUNT          307 a          710 c          633 g          344 t
ORIGIN      
        1 aacagtgcgt agcggccgcg caagcgctcg ccgtggaact cgaccatcac tttcgcctcg
       61 gtccacttct ccagctcgta ggtgccgctg tcccagacgc gcatcgtccc ggccccgtac
      121 tcgcccttcg ggatctcccc ctcccaggtc aggtactcga gcgggtggtc ctcggtgtgg
      181 accgccttgc ggttctcctt ggggtcgtac gggatcccgt tggggatcgc ccaggaagcc
      241 agagcgccgt cgtgctcgag tcgaaggtcc cagtgcagcc gggtggcgga gtgctcctgg
      301 atcacgaagc ggggagcgcc ggccgcgggc cgcgggtgtt ggggcgcagg ctcgggcgtg
      361 tcctgaaaat cgcgcttgcg ctcgtactct cgaagttcct tggccactcg atccaccgta
      421 cccgcgaccc gcggtccgcg gcccgcgacc ccgtgaaaac cggacgcttc gcaccctctc
      481 cgacaggtga ccttcatcta gggaacctcc gcaccgccct gctggcgtgg ctcttcgcgc
      541 ggtcggcggg cggcgcattc gtgctgcgcg tggaggacct cgaccgcccg cgcgtgcgac
      601 ccggcgcagg cgcacagatg ctggccgatc tccactggct cggcctcgat tgggacgaag
      661 ggccggatgt cggcgggccg cttggtccct acacccagag catacgccag gcgctctatg
      721 ctggcgcgct ggcccgcctg cgcgaccgcg acctgctcta cccctgctat tgcacacgcg
      781 ccgagctcca ggctctcgcc ggcatcgcca gcgcgcccca gggcggcgac gataccccgc
      841 gttaccccgg cacctgccgc gacctgagcg agcgccagcg ccgccagcgc gagcgggagg
      901 gacgccgccc cgcgctacgt ttccgtgtcc ccgacgccga gatcgccttt gacgatcaca
      961 tcgccgggcc gcgtcgggaa caggtcgccg cctcggttgg cgatttcgtg gtgcgacgca
     1021 gcgatggtat cgtcgcctac cagctcgccg tggtggtgga cgacgccctg atgggcatca
     1081 cccaggtggt gcgcggcgcc gacctgctca cctcaaccgc gcgtcagctt gccctcttcg
     1141 acgcgctcgg ctacgctcgg ccccagtcgt ttgcgcacgt gcccctggcg ctcgacccca
     1201 acggcgtccg tatggcaaag cgcgacgcct catccggcat cggcgaggcc cgcagcagcg
     1261 gcctagatca ccgcgttgtg ctcggcgcgc tggccgccag cgccggcatc tggccgcccg
     1321 gcgagaccgc cacccccgat caactcgttg ccgccttcga cccgcgcctg atcacatcca
     1381 cgccgacgcc tgtgagcctc gtctgagaga gtgccattcc ctttagtcgc gatttagttg
     1441 tgttagtcgc ggcgctcggt gcgcatcggc cggagacgcg caggcaatgt gtttggctcg
     1501 acgggacgcc attcgacatc gaacgcgccg cgatagccaa agagcggccc ccacgtgcgg
     1561 ttatgtacgt tcacgtcgat atgaaatcgc tggtcagtgt cgtcatacca ctcgcagaca
     1621 tcggcgattc ccgagaagaa cagcgggaat gagaacccga ctacgccttc gtagaggcgt
     1681 tgcgcccccg agcgaagacg caatccgccg cgctcgtcca ccgcgagatc cagatctacc
     1741 gccaggtgct cgtggctccc caggtaatcg acgatacgac cgcgccgctg gttatagctc
     1801 atgtaagcgt cgaaacgccg tgtcttgcgt gtcttgaacg tgcgcaccca ggtcacggtc
     1861 tcgcgcccaa cgtcgtccac aaaggcgtag ttttcaatcg tgaaggggac gttgcgcccg
     1921 tgttcgggaa acatgatgcg gcgccaactg ccgatataca gaaagggtag tgtgtagaac
     1981 gcgccgtgcc agat
//
LOCUS       DASVOR010000364         1629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208015,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000364 DASVOR010000000
VERSION     DASVOR010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1629
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208015"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..700)
                     /locus_tag="VF516_07470"
     CDS             complement(101..700)
                     /locus_tag="VF516_07470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8107552.1"
                     /translation="MAEPRDLMQRYCDGDAGAFRELYALVAPRLLGYLLKMTRARALA
                     DDLLQQTFLKVHRARAAYVRGADPVPWIYSIAHRTFIDETRRHRRAVVRVGEVEELPE
                     VPAGLTGESDDRRDEVRGDPELMTAALDALAELPAQQREAVVLTKLDGKSVAEAAEIA
                     GTTVGAMKVRAHRGYEALRKLLGRSAAPAKPAAGAGGGS"
     gene            791..958
                     /locus_tag="VF516_07475"
     CDS             791..958
                     /locus_tag="VF516_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107553.1"
                     /translation="MASGRRDKKRLKNRPKFKQERRLKGRRTKARKKAQHKRGLGRNR
                     ARRSRRGQKKK"
     gene            complement(1005..>1629)
                     /locus_tag="VF516_07480"
     CDS             complement(1005..>1629)
                     /locus_tag="VF516_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HEX8107554.1"
                     /translation="ERAAAGKTLDVYFDVASPFAYLGLTQLPALAAMGVRPRLVPILL
                     GALFRDIGQANVPLLAMPAAKQRYIGLDLARWASWWGEPFDFPRKFPQRTITAQRLCL
                     LAAAEGWEVGVRLATALGRAMWAEQRDLEDEQVLRAIVGAAGLPEDWVARTQEPAIKA
                     QLADHTTAARTAGVFGVPTYIVDGKHLFWGQDRLDLVARALAGWTPP"
BASE COUNT          242 a          596 c          580 g          211 t
ORIGIN      
        1 gacagcgccg gcggcttcgg cggcggcagc cgggacaggt catcgatcct ggcctcggcc
       61 tccggttcga gtcccgcgtc ggcgcccggt tcgatcgcgc tcacgatcca cctccggcgc
      121 cggcggcggg cttggccggc gcggccgacc tgccgagcag cttgcgcagc gcctcgtagc
      181 cgcggtgcgc ccgcaccttc atggcaccga cggtggtccc ggcgatctcg gcggcctccg
      241 ccaccgactt gccgtcgagc ttggtcagca cgaccgcctc gcgctgctgc gccggcagct
      301 cggcgagcgc gtcgagcgcg gcggtcatca gctcggggtc gccgcggacc tcgtcgcgcc
      361 ggtcatcgga ctcgccggtc aggccggccg gcacctcggg cagctcctcg acctcaccga
      421 cccggaccac cgcgcggcgg tgccggcggg tctcgtcgat gaacgtgcgg tgcgcgatcg
      481 agtagatcca cggcaccgga tcggcgccgc gcacgtaggc cgcgcgggcc cggtgcacct
      541 tcaggaacgt ctgctgcagc agatcgtcgg cgagcgcgcg cgcgcgcgtc atcttgagca
      601 gatacccgag cagccggggt gcgaccagcg catagagctc gcgaaatgct cctgcatcgc
      661 cgtcgcagta acgctgcatc aggtccctgg gctcggccat gcgaccgatg gcacgtatac
      721 gacagatcaa cccggtggtt acagttcttc gccccttgcc cggggcggcg gttttgtcga
      781 taactcgcgc atggcatcag gacgccgcga caagaaacgg ctcaagaacc gtcccaagtt
      841 caagcaggag cgacggctca agggtcgccg gaccaaggcg cgcaagaagg cgcagcacaa
      901 gcgcggcctg ggccgcaacc gggcccgccg gagccgccgc ggccagaaga agaagtagcc
      961 gaccgggcga tcggtcgggc gtgacgaggc gctgacgaaa accgtcaggg cggcgtccag
     1021 ccggcgagcg cccgggcgac caggtcgagg cggtcctggc cccagaacag gtgcttgccg
     1081 tcgacgatgt aggtcggcac gccgaacacg ccggcggtgc gcgcggccgt cgtgtgatcg
     1141 gcgagctgcg ccttgatcgc cggctcctgg gtccgggcga cccagtcctc gggcaggccg
     1201 gccgcgccga cgatcgcgcg cagcacctgc tcgtcctcca gatcgcgctg ctcggcccac
     1261 atcgcgcggc cgagcgcggt cgccaggcgc acgccgacct cccatccctc ggccgcggcc
     1321 agcaggcaca gccgctgtgc ggtgatcgtg cgctgcggaa atttccgcgg gaaatcgaag
     1381 ggctcgcccc accagctcgc ccagcgcgcg agatcgagcc cgatgtagcg ctgcttggcc
     1441 gccggcatcg cgagcagcgg cacgttggcc tgaccgatgt cgcgaaacag cgcgccgagc
     1501 aggatcggga ccagccgtgg tcggaccccc atcgcggcga gcgccgggag ctgggtcagc
     1561 ccgaggtagg cgaacgggct cgccacgtcg aagtacacgt ccagcgtctt gccggccgcc
     1621 gcgcgctcc
//
LOCUS       DASVOR010000365         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_441698,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000365 DASVOR010000000
VERSION     DASVOR010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441698"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF516_07485"
     CDS             <1..406
                     /locus_tag="VF516_07485"
                     /inference="COORDINATES: protein motif:HMM:NF020672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SelB C-terminal domain-containing protein"
                     /protein_id="HEX8107555.1"
                     /translation="DDRVRKASAPVRGATLSPAEAKLADQFRAWALEPPRPKDIPAEL
                     RLPEPQVKTMLDRLIAGKRLIRIKPDLYMHADVVADLRSRLVAFLDAHKTIDAQRWKE
                     LTGASRKFSIPLAEYFDAERVTLRVGDVRRKR"
     gene            complement(782..1627)
                     /locus_tag="VF516_07490"
     CDS             complement(782..1627)
                     /locus_tag="VF516_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107556.1"
                     /translation="MAVVRPAVAGPALDDNKAKPATAPVPAKEPSAPSPGAESSEPEP
                     DCSKPGDPRCAAVTGAVAGGEIEYGVGVRLRSVWLPRPVLELFVTRAAGGAHNYGLGV
                     DLTRRRGATELQLGFEFEHVNVGQGVWINKGDNVAMGDEADYVLGPDSSTGSGNQFGW
                     FTMEFSFFNHAEITPWLSFRYGAGLGIGVLIGELDHYNIICAMGATNASPSPGCVPSR
                     FNGTGMYTEGQETLVKYDLGTPVFPVVNAVLGLQLKPTDHTTINLEGGIRTMPFVGVS
                     SSMFF"
     gene            1872..>2204
                     /locus_tag="VF516_07495"
     CDS             1872..>2204
                     /locus_tag="VF516_07495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107557.1"
                     /translation="MNDICEPRCDRAPDCGDGYACRSGLCVGADGQRGDDCQGESDCA
                     AGLACQLKGAEVDPMTNRLVARCFAENSRGHPAGKPCGGDSDCRNGTCALGRCVDLCR
                     DTTDCAGGT"
BASE COUNT          349 a          804 c          728 g          323 t
ORIGIN      
        1 ggacgaccgc gtgcgcaagg cgagcgcccc ggtccgcggc gccaccctgt ccccggccga
       61 ggccaagctc gccgaccagt tccgcgcctg ggcgctcgag ccgccgcggc ccaaggacat
      121 ccccgccgag ctccgcctcc ccgagcccca ggtcaagacc atgctcgacc gcctgatcgc
      181 cggcaagcgc ctgatccgca tcaagcccga cctctacatg cacgccgacg tcgtcgccga
      241 cctgcgcagc cgcctggtcg ccttcctcga cgcccacaag acgatcgacg cccagcggtg
      301 gaaggagctc accggcgcca gccgaaagtt ctccatcccg ctcgccgagt acttcgacgc
      361 cgagcgcgtc accctccgcg tcggcgacgt ccgccgcaag cggtaaggta agacgagtag
      421 agaaaagaac agggaagaga gcatcggatc cgtaccgcgc ctcgagttcc agggccgcgc
      481 gacggacgcc gattcgacct tgtcctcgac gttgaccttg accaccaacg ttgcggttga
      541 cggcgacgat gacgtctgac cccacccttg accttgcacc ttgactccgg agtgggccat
      601 ggtcgcaatg tggacgacgg tcgaggtcga gtcgacgtcc tacgtcgctg tcaaccgcaa
      661 cgttcgtggt caacgtcgag gtcaaaccgt cgacctcgac ctccatctgc atcctgaatc
      721 gccactcgct tcttccttca acgcgcctga ttgatccggc cagctctcgt cgaccgcgag
      781 gctagaagaa catcgacgac gacacgccga cgaacggcat ggtgcggatc ccgccctcga
      841 ggttgatcgt cgtgtggtcg gtcggcttga gctgcaggcc gagcaccgcg ttgaccaccg
      901 ggaacaccgg cgtgccgagg tcgtacttga ccagggtctc ctggccctcg gtgtacatgc
      961 cggtgccgtt gaacctcgag gggacgcagc ccggcgaggg cgacgcgttg gtcgccccca
     1021 tcgcgcagat gatgttgtag tggtcgagct cgccgatcag gaccccgatg ccgaggccgg
     1081 cgccatagcg gaacgacagc cacggcgtga tctcggcgtg gttgaagaag ctgaactcca
     1141 tcgtgaacca gccgaactgg ttgccggagc cggtcgacga gtccgggccg agcacgtagt
     1201 cggcctcgtc gcccatcgcg acgttgtcgc ccttgttgat ccacacgccc tggccgacgt
     1261 tgacgtgctc gaactcgaag ccgagctgca gctcggtcgc gccccgccgc cgggtgagat
     1321 ccacgccgag cccgtagttg tgggccccgc cggccgcgcg ggtgacgaac agctcgagca
     1381 ccggcctggg gagccacacg ctgcgcagcc gcacgccgac gccgtactcg atctcgccgc
     1441 cggcgaccgc gccggtgacg gccgcgcagc gcggatcgcc gggcttgctg cagtcgggct
     1501 cgggctcgga gctctccgca cccgggctcg gggcgctcgg ctccttcgcc gggaccggcg
     1561 cggtcgccgg cttggccttg ttgtcatcga gcgccgggcc ggccaccgcc ggccgaacca
     1621 cggccatcag cgtgagcaag accaggcaaa ccacaggcga agcgaacggc atgggatccc
     1681 ccgagctgag ccgcattata tactcgcagc tctcgcctga ctcggtgccg ccctcgccga
     1741 tgcgacgcga tcccctctcc gcgctgatgt cgttcgccgc cctcggcgcc gcccttgtcg
     1801 ggtgtcgcgg gggggcgggg gtcggagaac cctgtggtag caacgacgac tgcgacggct
     1861 tgttgcagtg cttgaacgac atctgcgagc cgcgctgcga ccgcgcaccc gactgcggcg
     1921 acggctacgc ctgccgcagc ggcctgtgcg tgggcgcgga tggccagcgc ggcgatgact
     1981 gccagggaga gtccgactgc gccgccggcc tggcgtgcca gctcaagggc gccgaggtcg
     2041 accccatgac caaccgcctg gtcgcgcgct gcttcgccga gaactccagg gggcacccgg
     2101 ccgggaagcc gtgcggcggc gacagcgact gccgcaacgg gacctgcgcg ctcggccgct
     2161 gcgtcgatct gtgccgcgac accacggact gcgccggcgg cacc
//
LOCUS       DASVOR010000366         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_324849,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000366 DASVOR010000000
VERSION     DASVOR010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324849"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            393..1463
                     /gene="recA"
                     /locus_tag="VF516_07500"
     CDS             393..1463
                     /gene="recA"
                     /locus_tag="VF516_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEX8107558.1"
                     /translation="MSNQDKTIHDKKNKSIAEAIRAIHKQFGSGSIMRLDGSEVQPVE
                     VIPTGSVALDVALGCGGLPRGRIIEIYGPESSGKTTLTLHAIAEAQRAGGVCAFIDAE
                     HALDTDYARRLGVDLEDLLVSQPDCGEQALEITDTLVRTGAIDLIVVDSVAALTPRAE
                     IEGEMGDAHMGLQARLMSQALRKLTAQISRTRTVVVFINQLRQKIGVVYGNPETTTGG
                     NALKFYCSVRLDIRRKKAIKRGDETIGSECKVKVVKNKLAPPFREADFEILYGTGVNK
                     LGELVDTAEKLGVMEKSGTWYSYDGAKLGQGRDKVLAHLDEHPAMQQQLRDQLIKQAR
                     LAATTPPSAVAAAAIASQGAVS"
     gene            1460..>1834
                     /locus_tag="VF516_07505"
     CDS             1460..>1834
                     /locus_tag="VF516_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107559.1"
                     /translation="MTRDATPPTPPIPGTPPALAADEAAVLLDRLIYEQVVACIDLEE
                     LHALEQAITLLAAELDGVGPDHAADLATAMIDAALRRLPDDVRRYLRGARWLFDDRCE
                     LCEDEARADRRDSSHPHRGTRPR"
BASE COUNT          293 a          714 c          578 g          249 t
ORIGIN      
        1 gcggcgcgct tgtggtcgcc ctggcgcccc ggccggcggc gcgctcgcgg tcgccctggt
       61 gcccccggcc ggcggcgcgt tcgcggtcgc tctaattcat catcgaatcg tgcggggcgc
      121 gccggggctc ggatcgcttc cgccatttct tcgccagaca ccaggggatc cagcggcacc
      181 gaggggatcc cggcgggttt cgacccggct ccaagtacgc gagagagctg cagagcgtcg
      241 acgacagacg ggtttctctc tcgtgactgt ccgggtttcg tccggattcc ggacgacccc
      301 gtccgccggc tggacatttc ggacagccgc cctcgcgatc tcgcagggtt gcgatggcac
      361 acgacgtgga atccccccgc gcggaggttt tcatgtcgaa ccaggacaag acgattcacg
      421 acaagaagaa caagagcatc gccgaggcga tccgcgccat ccacaagcag ttcggctcgg
      481 gctcgatcat gcggctcgac ggcagcgagg tccagccggt cgaggtcatc ccgaccggct
      541 cggtcgccct cgacgtcgcg ctcggctgcg gcggcctgcc gcgcggccgg atcatcgaga
      601 tctacggccc ggagtcgtcg ggcaagacca ccttgaccct gcacgcgatc gccgaggccc
      661 agcgcgccgg cggcgtgtgc gcgttcatcg acgccgagca cgcgctcgac accgactatg
      721 cgcgccggct cggcgtcgac ctcgaggacc tgctggtctc gcagcccgac tgcggcgagc
      781 aggccctgga gatcaccgac acgctggtcc gcaccggcgc gatcgacctc atcgtcgtcg
      841 actcggtcgc cgccctgacc ccgcgcgccg agatcgaggg cgagatgggc gacgcccaca
      901 tgggcctgca ggcccgcctg atgagccagg cgctgcgcaa gctcaccgcg cagatctctc
      961 gcacccgcac ggtcgtcgtg ttcatcaacc agctgcggca gaagatcggc gtggtctacg
     1021 gcaaccccga gaccaccacc ggcggcaacg ccctcaagtt ctactgctcc gtccgcctcg
     1081 atatccgccg caaaaaagcc atcaagcgcg gcgacgagac catcggcagc gagtgcaagg
     1141 tcaaggtcgt caagaacaag ctcgctccgc cgttccgcga ggccgacttc gagatcctct
     1201 atggcaccgg cgtcaacaag ctcggcgagc tcgtcgacac cgccgagaag ctcggcgtca
     1261 tggagaagtc cggcacctgg tactcctacg acggcgccaa gctcggccag ggccgcgaca
     1321 aggtcctcgc ccacctcgac gagcaccccg ccatgcagca gcagctccgc gatcagctga
     1381 tcaagcaggc ccggctcgcc gctactaccc caccttcggc cgtcgccgcc gccgccatcg
     1441 ccagccaggg ggccgtgtcg tgacccgcga tgccaccccg cccacaccac ccatcccggg
     1501 caccccgcct gcgctcgccg ccgacgaggc cgccgtcctg ctcgaccgcc tgatctacga
     1561 gcaggtcgtc gcctgcatcg acctcgagga gctccacgcc ctcgagcagg cgatcaccct
     1621 gctcgccgcc gagctcgacg gcgtcggccc cgaccacgcc gccgacctcg ccacggccat
     1681 gatcgacgcc gccctgcgcc gcctgcccga cgacgtgcgc cgctacctgc gcggcgcccg
     1741 ctggctgttc gacgaccgct gcgagctctg cgaggacgaa gcgcgcgccg accgccgcga
     1801 cagcagccac ccgcaccgcg ggacccggcc cagg
//
LOCUS       DASVOR010000367         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_120222,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000367 DASVOR010000000
VERSION     DASVOR010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120222"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VF516_07510"
     CDS             complement(<1..925)
                     /locus_tag="VF516_07510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107560.1"
                     /translation="MTDSSSRSAPVKTGDVLDGRYRIVRTLGGGSMGTVYLAEHVLIK
                     RQVAIKILRPDIGDGAVLDAFMAEARAAGTLGHPNIVACTDQGFTGGGAPYLVLEYLE
                     GAVLTDEIYRLGGLPVRRALRIAEQIASALRVAHEVGIVHRALTSDNVFLVDKSHARD
                     AVKVLDFGVARCFEAQGSTRRGALLSAPEFMAPEQLTSPDTVDPRADVYALGVILYEM
                     LTARRPFSDDDRRSLLHRVVSEQPPPLLRPDVLPELEDMIVSRLLAKDPAQRHASMKE
                     VQSALDAFVAAARPSGGSSGEIQLPPPDLLPA"
     gene            1312..2088
                     /locus_tag="VF516_07515"
     CDS             1312..2088
                     /locus_tag="VF516_07515"
                     /inference="COORDINATES: protein motif:HMM:NF042851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HEX8107561.1"
                     /translation="MRTVRAIRYLTPLREGGSVPAIVEADDDGTYVAKFRGAAQGPRA
                     LVAELIAGELGRLLGLPVPQLVLIDVDAVLARSEPDPEIQGLLRNSAGLNVALDYLPG
                     ALNWERGLAPPPDRELAAAVVWFDALITNVDRTPRNPNLLRWHRALYLIDHGAALYFH
                     HDWPKTAADRLARSRTPFAMIRNHALLPLAGDLAAADAALAPRITEAALAAIVAEVPD
                     DWLTGDEPPEAIRRGYVEHLWSRLAVPRLFVEEATRAKLV"
     gene            2072..2467
                     /locus_tag="VF516_07520"
     CDS             2072..2467
                     /locus_tag="VF516_07520"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEX8107562.1"
                     /translation="MPSSFDHALIRVVPRVERGELINAGAVVSCPTQGYLAARIALDA
                     ARLVALWPAVDVAEVEVALALFPLIAAGDPRGGPIAALPRGERFHWLVAPRSAVIQTS
                     PVHTGLCDDPGAALDHLIQRLVAVHRDVR"
     gene            2790..3395
                     /locus_tag="VF516_07525"
     CDS             2790..3395
                     /locus_tag="VF516_07525"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX8107563.1"
                     /translation="MTLNSCLAHAPFRDHYHVCAFVMGPSEERALIDPFFVEGLRRGE
                     KAVYYVDPAQRDEHEARLRTSAPSPDMVEVTTWHDAYFKGGSFDPDRMMAALEDMIRD
                     NAASGNPPVRLVGQMGWMFSSLPGIEQLVAYEASVNQVLDRGKTPTVCVYDVRRLSGS
                     MMMDLLRAHPLTVVNGVLHENPFYTPADEMLRDLQRRRAMS"
     gene            3400..>3693
                     /locus_tag="VF516_07530"
     CDS             3400..>3693
                     /locus_tag="VF516_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107564.1"
                     /translation="MAGLSPSDSGEDESDRRLDRCIRDLAALNALPSMCIGRSPGEAL
                     GIVVDALPTALSCDLVYLALPGSPPAERAIVRGAQVPEAELPALAAAIGRGAGD"
BASE COUNT          509 a         1290 c         1328 g          566 t
ORIGIN      
        1 ccgcgggcag gagatcgggt ggcggcagct ggatctcgcc cgagctcccc ccgctcggcc
       61 gggcggcggc cacgaacgcg tcgagcgcgc tctgcacctc cttcatcgag gcgtggcgct
      121 gcgccggatc cttggcgagc aggcgggaga cgatcatgtc ctcgagctcg ggcagcacgt
      181 cgggccgcag cagcggcggc ggctgctcgc tcaccacgcg gtgcagcagc gagcggcgat
      241 cgtcgtcgct gaacggccgg cgcgcggtca gcatctcgta gaggatcacg ccgagcgcat
      301 agacgtcggc gcgggggtcg acggtgtcgg ggctggtcag ctgctcgggc gccatgaact
      361 cgggggcgct gagcagcgcg ccgcgccggg tgctgccctg ggcctcgaag cagcgcgcga
      421 cgccgaagtc gagcaccttc acggcgtcgc gcgcgtggct cttgtcgacc aggaacacgt
      481 tgtccgaggt cagcgcgcgg tggacgatgc cgacctcgtg cgcgacccgc agcgccgacg
      541 cgatctgctc ggcgatccgc agcgcgcgcc ggaccggcag gccgccgagt cggtagatct
      601 cgtcggtgag gacggcgcct tcgaggtact cgaggacgag gtacggcgcg ccgccgccgg
      661 tgaacccctg gtcggtgcac gcgacgatgt tggggtgacc cagcgtcccg gcggcgcggg
      721 cctcggccat gaaggcgtcg agcaccgcac cgtcgccgat gtcgggtcgc aggatcttga
      781 tcgcgacctg gcgcttgatg agcacgtgct cggccagata cacggtcccc atcgagcccc
      841 cgccgagcgt gcgcacgatg cgatagcgcc cgtcgaggac gtcgccggtc ttgacgggag
      901 cacttcgact agaagaatcc gtcaccacaa gaatctcgat gatccgatgt cggccgcggc
      961 gagtaaagcg ggaacctacg agaggcgctg agggatgctg ctgtgcgaag tcatcgcacg
     1021 aaatcgagcc gcggagactc cctttgcgca cctgcggcca aaagccccac gtcctggatg
     1081 gcgcaccgcg tgaccgtcag ggcgcctcac gcaggacgcg gcagcccccg ggctccagcg
     1141 cacagctgcg cgacctggca cccccaccgt cttgcgttcg tggatcgcct gcgaccgatc
     1201 ggtcacgccc aggctggcac ccaggctggc acccaggctg gcaccgagcg cggcgagcgg
     1261 gtcgcgccac gacgcgaggt ggtcggccgg gctcggggta gattcgcccg gatgcgcacg
     1321 gtccgcgcga tccggtacct gacgccgctg cgcgagggcg gctccgtgcc ggcgatcgtc
     1381 gaggccgatg acgacggcac ctacgtcgcc aagttccgcg gtgcggcgca ggggccgcgc
     1441 gccctggtcg ccgagctgat cgccggcgag ctcggccggc tgctcggcct gccggtcccg
     1501 cagctcgtgc tgatcgacgt cgacgccgtg ctcgcgcgct ccgagcccga tccggagatc
     1561 cagggcctct tgcgcaacag cgccgggctc aacgtcgcgc tcgattacct gccgggtgcg
     1621 ctcaactggg agcgcggcct cgccccgccg cccgatcgcg agctcgccgc cgccgtggtg
     1681 tggttcgacg cgctgatcac caacgtcgat cgcacgccgc gcaaccccaa cctcctgcgc
     1741 tggcaccgcg cgctgtacct gatcgatcac ggcgcggcgc tgtacttcca ccacgactgg
     1801 ccgaagaccg ccgccgatcg cctggcgcgc agccggacgc cgttcgcgat gatccgcaac
     1861 cacgcgctgc tgccgctcgc cggcgacctc gcggccgccg atgccgcgct cgcgccgcgg
     1921 atcaccgagg ccgcgctggc ggcgatcgtc gccgaggtcc ccgacgactg gctgaccggc
     1981 gacgagccgc ccgaggcgat ccggcgcggc tacgtcgagc acctgtggtc gcggctcgcg
     2041 gtgccccggc tgttcgttga ggaggcgacg cgtgccaagc tcgtttgacc acgcgctgat
     2101 ccgggtcgtg ccgcgggtcg agcgcggcga gctgatcaac gcgggcgcgg tcgtgtcgtg
     2161 cccgacccag ggctacctcg cggcgcggat cgcgctcgac gccgcgcggc tggtcgcgct
     2221 gtggcccgcg gtcgacgtcg ccgaggtcga ggtcgcgctc gcgctgtttc cgctgatcgc
     2281 ggcgggcgat ccgcgcggtg gcccgatcgc ggcgctgccg cgcggcgagc ggttccactg
     2341 gctggtcgcc ccgcgcagcg cggtgatcca gacctctccg gtgcacaccg ggctgtgcga
     2401 cgatcccggc gctgcgctcg accacttgat ccagcggctg gtcgcggttc atcgcgacgt
     2461 ccgatgaccg ccgacagcga aagccgcgat cgacggcttc ggctcgtatc caggcgcgca
     2521 gtggagaatc gctgtcgagt gcgcgattcc actcctggcg atgatgatgg ggtatcggtg
     2581 ccacgtcgat gtcggcctcg ccgtgatgtg gagtcaacga gaactcccgg gattggtact
     2641 tgctggacgc gattgcgtgg tgcggtgtgg ctccggcgca cggtcgatgc cagcctgcat
     2701 ggattctcgc acgagaccgt ctcgctccct ggcgacgcat tcgccggcat cgccgtcccg
     2761 tgcagttgcg tgcgtgctac gttgccgacg tgacgctgaa tagctgtctg gcccacgcgc
     2821 catttcgcga tcattatcac gtctgcgcct tcgtcatggg gccatccgag gagcgggcgc
     2881 tgatcgatcc gttcttcgtc gagggactgc ggcgcggtga aaaggcggtg tactatgtcg
     2941 atccggcgca gcgggacgag cacgaggcgc gcctccggac gtccgcacca tcgcccgaca
     3001 tggtcgaggt gacgacctgg cacgacgcat acttcaaggg ggggtcgttc gaccccgatc
     3061 ggatgatggc ggcgctcgag gacatgatcc gcgacaacgc tgcgagcggg aacccgccgg
     3121 tccggctggt cggccagatg ggctggatgt tctcctcgct gcccggcatc gagcagctcg
     3181 tcgcgtacga ggcgagcgtc aaccaggtcc tcgatcgcgg caagaccccg acggtctgtg
     3241 tctacgacgt gcggcgcttg agcggttcga tgatgatgga tctgctgcgc gcccatcccc
     3301 tcaccgtggt caacggcgtc ttgcacgaga acccgttcta cacccccgcc gacgagatgc
     3361 ttcgcgatct ccagcggcgg cgcgcgatga gctgagtcga tggctggatt gtcgccgagc
     3421 gactcggggg aggacgagag cgatcgtcga ctggaccgct gtatccggga cctggcagcg
     3481 ctcaacgcgt tgccgtcgat gtgcatcggg cggtcccccg gggaggcgct cggcatcgtg
     3541 gtcgatgcgc tgccgaccgc gctgagctgc gatctggtgt acctggcgct gccgggctca
     3601 cctcccgccg agcgcgccat cgtgcgcgga gcgcaggtcc ccgaggcgga gctcccggcg
     3661 ctcgcggcgg cgatcggccg cggtgccggt gac
//
LOCUS       DASVOR010000368         2639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_411965,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000368 DASVOR010000000
VERSION     DASVOR010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2639
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411965"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1953
                     /locus_tag="VF516_07535"
     CDS             <1..1953
                     /locus_tag="VF516_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8107565.1"
                     /translation="HERPADRSGPPGADAVRQAMATLWSATPASASGGVPLPADVQEP
                     MSRSFGVDFSDVRIHENSASATALNALAYTRGVEIHFAPGQYAPATAAGRELLGHELV
                     HVVQQSHGRTGATAQRRGETISADHALEHEADDLGARAARGESLGLRRGSGGAQNVQL
                     KPASALKPADKGRRFRVQLLPGRNETRIGMYLYSDLSGHTFREERDDEIREFIVTSPS
                     VEVTPLDDNDHQTTSGGPSSGPGGPGGLSGVKAPPLLGLGCDGLTAEQVLTGIRCGYR
                     MFDLAVSYRNHKQVTAAIRAALEQRLVARSELFLIFKFLPFNDGDVAQQVFAVIEQAL
                     GDLGVNSLDCVMLHAPASKNLAAHLKALKHLVGIGKVLQLGLSNVDVSQLQAIHHSAD
                     APKISFVENEFSPLKLDVQTLEACVQSGIRFIGYSVLGGRLRKDQILNNEALLELAEK
                     FHVDVAQLVLAWAISLGVHQIPKSTDVEHMTANLTALELAGSGPLMAMVAHVIKLEFA
                     DKKHDHDAPVLELHDRPEDDKESEKESEKESDSSKKRKSEEREGEKPTVNPVQTLCEL
                     MMELANNRKDDDRVNGCLKVIQEHLETPPMMKLLKNWRRSSTRDPSLARILKLIEFLA
                     SPDPSLGTCDIVKAIDELLPLLAQFL"
     gene            complement(2030..>2639)
                     /locus_tag="VF516_07540"
     CDS             complement(2030..>2639)
                     /locus_tag="VF516_07540"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8107566.1"
                     /translation="MALADLAGVLRHAVGDREVCLTHVSLSWHGALWDFGHPLDYIGS
                     DGGAGMGAGPGISVGAALALDGSGRLPIAICGDGDFMMGCTAIWTAAHYRIPLLIVVA
                     NNRSFYNDEVHQERMAIARNRPVENKWIGQRIADPDIDIAQIARAQGAHGFGPAHTAS
                     QLEAALNEAIAAVERGGVAVVDARIIASYSAASFANLARRTP"
BASE COUNT          460 a          906 c          887 g          386 t
ORIGIN      
        1 cacgagcgcc ccgccgatcg cagcgggcca cccggcgccg acgccgtgag gcaggcgatg
       61 gccacgctgt ggtcggccac gcccgcctcg gcgtccggcg gcgtcccgct cccggcggat
      121 gtgcaagagc ccatgtcccg ctcgttcggc gtcgacttct ccgatgtccg gatccacgag
      181 aactcggcca gcgcgacggc gctgaacgcg ctggcctaca cgcgcggagt cgagatccac
      241 ttcgcgccgg gccagtacgc acccgccacc gcagccggac gggagcttct gggacacgag
      301 ctggtccacg tcgtccagca atcgcacggc cggaccggcg ccaccgcgca gcggcgaggc
      361 gagacgatca gcgccgatca cgcgctcgag cacgaggccg acgacctggg cgcgcgggcg
      421 gcgcgcggcg agtcgctggg gctgcgccgg gggtcggggg gtgcgcagaa cgtacagctc
      481 aagccggcgt ccgcgctgaa gcctgccgac aagggccggc gctttcgcgt gcagctcctg
      541 ccggggcgca acgagacgcg catcggcatg tacctttaca gcgacctcag cggccacacg
      601 ttccgcgagg agcgcgacga cgagatccgc gagttcatcg tgacatcgcc cagcgtcgag
      661 gtcactccgc tcgacgacaa cgaccaccag accacgagcg gtggaccgag ctccgggccc
      721 ggcggaccgg gtggcctgag cggagtgaag gctccgccgc tcctgggcct cggttgcgac
      781 ggcctcactg ccgagcaggt gctcaccggg attcgctgcg gataccgcat gttcgatctg
      841 gcggtgtcct acaggaatca caagcaggtg accgcggcga tccgcgcggc gctcgagcag
      901 cggctcgtgg cacgcagcga gctgttcctg atcttcaagt tcctgccatt caatgacggc
      961 gacgtggcac agcaggtctt cgcggtgatc gagcaggcgc tcggtgacct cggggtgaac
     1021 agcctcgact gcgtcatgct gcacgcgccg gcgtccaaga acctggccgc gcatctcaag
     1081 gcgctgaagc acctggtcgg gatcggcaag gtgctgcagc tcggcctcag caacgtcgat
     1141 gtgtcgcagc tccaggccat ccaccactcc gcggacgcgc cgaagatctc gttcgtggag
     1201 aacgagttct cgccgctcaa gctcgacgta cagacgctcg aggcctgtgt ccagtcgggc
     1261 atccggttca tcggctacag cgtcctcggc gggcggctgc gcaaggacca gatcctgaac
     1321 aacgaggcgc tcctcgagct ggccgagaaa ttccacgtcg acgtggcgca gctcgtcctc
     1381 gcgtgggcga tctccctcgg ggtgcaccag atccccaaga gcaccgatgt cgagcacatg
     1441 accgccaacc tgaccgcgct ggagctcgcc ggcagcggtc cgctcatggc gatggtcgca
     1501 cacgtgatca agctcgagtt tgcggacaag aagcacgacc acgacgcgcc tgttctcgag
     1561 ctgcacgacc gccccgagga cgacaaggag agcgagaagg agagcgagaa ggagagcgac
     1621 agcagcaaga agcgcaagag cgaggagcgc gagggcgaga agcccacggt gaacccggtg
     1681 cagacgctct gcgagctgat gatggagctc gcgaacaatc gcaaggatga cgatcgggtg
     1741 aatggttgct tgaaggtcat ccaggagcat ctcgaaaccc cgccgatgat gaagctcctg
     1801 aaaaactggc gacgctcgag cacgcgggac ccctcgcttg cacggatcct caagctcatc
     1861 gagttcctcg ccagccccga tccatccctg ggcacctgcg atatcgtgaa ggcgatcgac
     1921 gagctgctgc cactccttgc gcagttcctg tagccaatcc gaccggcgat cgatccgcgc
     1981 ccccggcgat gctcgatggc gcgagtcttc cggtcgtcgc ggtgctccgc tacggcgtcc
     2041 tgcgcgccag gttcgcgaag ctcgccgcgc tgtagctcgc gatgatccgc gcgtcgacca
     2101 cggccacccc accgcgctcg accgcggcga tcgcctcgtt cagcgcggcc tcgagctgcg
     2161 aggccgtgtg cgccgggccg aagccgtgtg cgccctgcgc ccgtgcgatc tgtgcgatgt
     2221 cgatgtccgg atcggcgatg cgctggccga tccacttgtt ctcgaccggg cggttgcgtg
     2281 cgatcgccat ccgctcctgg tggacctcgt cgttgtagaa cgagcggttg ttggcaacga
     2341 cgatcagcaa tgggatcctg taatgtgcgg cagtccagat cgccgtacac cccatcatga
     2401 aatcaccatc gccgcagatg gcgatcggca ggcggccgga gccgtcgagc gccagcgctg
     2461 cgccgaccga gattccgggg ccggcgccca tgcccgcgcc gccgtccgag ccgatgtagt
     2521 cgagtggatg cccgaagtcc cagagcgcgc catgccagga cagcgacacg tgggtgagac
     2581 agacttcgcg atcgccgacc gcgtggcgca gcacgccggc caggtcggcc agggccatc
//
LOCUS       DASVOR010000369         4805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_500366,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000369 DASVOR010000000
VERSION     DASVOR010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4805
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500366"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(829..1788)
                     /locus_tag="VF516_07545"
     CDS             complement(829..1788)
                     /locus_tag="VF516_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107567.1"
                     /translation="MKPVYTIALLALVACDSKPPDQALATAIANGLAAACPAGADPSD
                     EKARNDCADKLTNLAVLRDAQREPFIWGGQQPGKGYALDTKTNKFNARVWRRMYLSTF
                     MFGTDYSIEQVGDTTVLHVPIMFRGAMSTGAYPYPFWHSEKKWNAYNYATTAHFIIQN
                     GLVIGALRGTDQDPTRPKVAHAWDGLWQWKQDGVQMPYVSFYDYLLSKGNPHARELND
                     AYRAMELRMRQNACQACHAPDDQGKSDQLEFFTYPNQALAGRHDLVTQLEANAMPPED
                     NTLKLPAGIADPAEREALLKLARDFEIAGDQALAWEGENKTQF"
     gene            complement(1797..2504)
                     /locus_tag="VF516_07550"
     CDS             complement(1797..2504)
                     /locus_tag="VF516_07550"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8107568.1"
                     /translation="MASLAGCAENQVTHTAPETPTTGTDRATVIGHVLDTTGAPVVGA
                     TVTVRSSGEHATVDSTGAFTLDVPANTTLTLSATAPDMAPTLLQQFLVSTGTTAQVEI
                     PLLTSDHFKSLVAMGMNPSGGVVAVAVKSLSGAGSAAGATVQLTPSLGRMMYAPAGAG
                     MADPDPAMAAVVPSVAQGDESYAWALGVQPHVSIMQFALHGVAEIEPPYAIDDVIWPG
                     TFTVDAGALTLVTLFTK"
     gene            complement(2987..4105)
                     /locus_tag="VF516_07555"
     CDS             complement(2987..4105)
                     /locus_tag="VF516_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX8107569.1"
                     /translation="MDRNAFLKLLAFGVERGVSDIHFEVGYPPHYRLHGELHGALKVP
                     PLAAQDTEAIARMILEDHGITVDFTRRFAEIDVAYALAQRGRFRASIFRQRGAVGIVM
                     RLVPIQALSIAQLNLPPVLAEIADARRGLVLVTGATGNGKTTTMAAMLRHINETRHAH
                     IVTIEDPIEFLHEPQKCMIIQREVGTDTDSFSGALTAALRQDPDVLLVGEIRDRETAA
                     TCLKAAETGHLVLGAIHTPDAVATVQLYLGLFATGAMDAIRERLGDGLQAIISLRLLP
                     AKDGGRRLPAVEILRVTRAIRDCLRGGRMSDLPELIRKGRDLYSMQLFDQHLLDLVNA
                     GLVSMEAAIDASSNPEELGARCASSEGTSLDPGAEPER"
     gene            complement(4113..>4805)
                     /locus_tag="VF516_07560"
     CDS             complement(4113..>4805)
                     /locus_tag="VF516_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8107570.1"
                     /translation="VAIDDVGTRHSNLETVMSLRPHFIKISDVLIRGIARSTVKREML
                     RSLRHIAETIDAVMIAEGIERLDDVVALRDLGLRYGQGYYMARPAQGFPTVPDDVRGE
                     LRNVTSAVPDVALVATDDEDDDDDRDGPLAIGSSRRMLADALVRNAFGRSEDEITKDF
                     QIPHEDPSPESSGMHPVRGEPVAAAWRPLTDDDLGATPEAEPLLDSLRRAQTVPDTVP
                     DDEGGGGRPGLN"
BASE COUNT          674 a         1640 c         1729 g          762 t
ORIGIN      
        1 cctcgtcgag cagcgcgcac ccggcccacc cgcgatagcg cgagctcatc gctgcggggg
       61 cgcggccgcg gctaccgccg ctacccgcta ccgcttcggc tgccgctgcc gctgccggct
      121 gccgctgccg ctcgccggta ccgctcgccg ctcgccgctg ccgcttcgcc tccggctact
      181 gctgccgctc cgtcggtgag catgcaccgc gacggtatgg ccctcctgcg agtgccctca
      241 cgccgcgccg ctcgatgcgc tcacagcgag cctccgtgat cgcccggcgc accgggggcg
      301 agttcacagg aggttccatg ttcgatacgc tcgagatgtc actcaccgtg ctggagcagc
      361 tcgctcccgt cgagaccaag atccgccagc gcgcaagtcg ctcgccgacg aggtcggccg
      421 cgccgacgaa tcggtcgcgc tcaacgtccg cgaagcacgg cagcgcgccg gcctcgatcg
      481 cgccgacctg ttccgccggg ccgccggctc ggccagcgag ctcaccaccg cgctgcgcat
      541 cgcgcgcgcc cgcggctaca tcaccgcggc cgagcacgcc gcggtcgaag ccgcgctgga
      601 tcgggttcgc gcgatgctgt ggcggctcac ccactgagca tcgactcggg ctacctcggg
      661 cccgcgaggc gcgagccccg tggatcgaca agcgacaacg tgaagagagg acttcgaatg
      721 aagtgcggcc gggccagctc gatgggagag ctggcccggc ccgggtcgta tcttgctcac
      781 agactgtgac gggtgcttgc agccgtatcc actcgaacgt tcacgtatct agaactgagt
      841 cttgttctcg ccctcccatg cgagtgcctg gtcaccagcg atctcgaagt cacgcgcgag
      901 cttgagcagc gcctcgcgct ccgccggatc cgcgatcccg gccggcagct tgagcgtgtt
      961 gtcctccggc ggcatcgcgt tcgcctcgag ctgcgtgacg aggtcgtggc ggccggcgag
     1021 cgcctggttg gggtaggtga agaactcgag ctgatccgat ttgccctggt catccggcgc
     1081 gtggcaggcc tggcaggcgt tctgccgcat ccgcagctcc atcgcgcggt acgcgtcgtt
     1141 gagctcgcgg gcatgcgggt tgcccttcga cagcaggtag tcgtagaacg acacgtacgg
     1201 catctgcacg ccgtcctgct tccactgcca caggccgtcc caggcatgcg cgaccttggg
     1261 ccgcgtgggg tcctgatccg tgccgcgcag cgcgccgatc acgaggccgt tctggatgat
     1321 gaagtgggcg gtcgtcgcgt agttgtaggc gttccacttc ttctcgctgt gccagaacgg
     1381 atacgggtag gcgccggtcg acatcgcacc gcggaacatg atcgggacgt ggagcaccgt
     1441 ggtgtcgccg acctgctcga tcgagtagtc ggtaccgaac atgaacgtcg acaggtacat
     1501 ccggcgccac acgcgggcgt tgaacttgtt ggtcttggtg tcgagcgcgt agcccttgcc
     1561 cggttgctgg ccgccccaga tgaacggctc gcgctgcgcg tcgcgcagca cggcaaggtt
     1621 ggtgagcttg tcggcgcagt cgttgcgcgc cttctcgtcg gacgggtcgg cgcccgcggg
     1681 gcacgccgct gccagtccgt tcgcgatggc cgtcgcgagc gcctgatccg gtggcttgga
     1741 atcacacgcg acgagcgcga ggagagcgat ggtgtagacg ggcttcatcg ggatcctcac
     1801 ttggtgaaca gcgtgaccag ggtgagcgcg ccggcgtcga cggtgaacgt gcccggccag
     1861 atgacgtcgt cgatcgcgta cggcggctcg atctcggcga cgccgtgcag cgcgaactgc
     1921 atgatggaga cgtggggctg gacgccgagc gcccaggcgt aggactcgtc gccctgcgcg
     1981 accgacggca cgaccgctgc catcgcgggg tccgggtcgg ccatcccggc gccggccggc
     2041 gcgtacatca tgcggcccag gctcggggtg agctggacgg tggcacccgc ggcgctgccg
     2101 gcgcccgaca gcgacttcac cgcgacggcg accacgccgc ccgaggggtt catgcccatc
     2161 gcgaccaggc tcttgaagtg gtcgctggtg agcaggggga tctccacctg ggcggtggtg
     2221 ccggtcgaga ccaggaactg ctggaggagg gtgggcgcca tgtcgggcgc ggtcgccgag
     2281 agggtcagcg tggtgttggc ggggacgtcg agggtgaacg cgcccgtgct gtccacggtg
     2341 gcgtgctccc cggagctgcg gacggtcacc gtcgcgccca ccacgggggc gccggtggtg
     2401 tcgaggacgt ggccgatgac ggtcgcgcgg tccgtgccgg tggtgggcgt ctcgggggcg
     2461 gtgtgggtca cctggttctc tgcgcaaccc gcgagcgagg ccatcacgac aacgaacgga
     2521 gcaactcgat tcctcatgac ggacaggcct cattgcacgg gcaatgccgg agtcatcgcc
     2581 gtcgcgcagc ttgcggtgac gcgccggcgg atctcgatgg ctttcgaaag cttcctgaaa
     2641 ctccggacgc tcggcggtgc gatcgcgagc ggctcctcgc cccggacatc ctcacgcgga
     2701 ccccgagggc cattcgggag cgctcgacgc ggcacggcag gagcgactcg aacgcggaca
     2761 gctctctcga cctcacgctc aactgccctc aacttcatgc acaaattccc ctgacgcggt
     2821 tgacagctcg atccgtggct cgcgatcggc gggccggcta actgcgttca ccgagggcgc
     2881 cacgccgaaa gttcgggggg aacaacggat gtccaggggg tgcccccggc gagttccatc
     2941 agcccgtgcg ccttgtcctc ggatccgcgc tcagtggaat tgtgcttcag cgctcgggtt
     3001 cagcgccggg gtccagcgag gtcccctcac tcgacgcgca gcgtgccccg agctcctcgg
     3061 ggttcgagct cgcgtcgatc gcggcctcca tcgacaccag gccggcgttg accaggtcga
     3121 gcaggtgctg gtcgaacagc tgcatcgagt acagatcgcg gcccttgcgg atcagctcgg
     3181 ggaggtcgct catccggccg ccgcgcaggc agtcgcggat cgcgcgggtg acccgcagga
     3241 tctcgaccgc gggcagccgg cgcccgccgt ccttggcggg caacaggcgc agcgagatga
     3301 tcgcctgcag accatcgccg agccgctcgc ggatcgcgtc catcgcgccg gtggcgaaca
     3361 ggccgaggta gagctgcacg gtcgcgaccg cgtccggcgt gtggatcgcg cccagcacca
     3421 ggtgaccggt ctcggcggcc ttgaggcagg tcgccgcggt ctcccggtcg cggatctcgc
     3481 cgaccaggag gacgtcgggg tcctggcgca gcgccgcggt cagcgcgccc gagaagctgt
     3541 cggtgtcggt gccgacctcg cgctggatga tcatgcactt ctggggctcg tggaggaact
     3601 cgatcggatc ctcgatcgtc acgatgtggg catggcgggt ctcgttgatg tggcgcagca
     3661 tcgccgccat cgtcgtggtc ttgccgttgc cggtcgcgcc ggtgaccagc accaggccgc
     3721 ggcgcgcgtc ggcgatctcg gcgagcaccg gcggcaggtt gagctgcgcg atcgaaagcg
     3781 cctggatcgg aacgaggcgc atcacgatgc cgaccgcgcc gcgctggcgg aagatcgacg
     3841 cgcggaaccg tccgcgctgg gccagcgcgt acgccacgtc gatctcggcg aaccgccggg
     3901 tgaagtcgac ggtgatgccg tggtcctcga ggatcatccg ggcgatcgcc tcggtgtcct
     3961 gcgcggcgag cggcgggacc ttgagcgcgc cgtgcagctc gccgtgcagc cggtagtgcg
     4021 gcgggtagcc gacctcgaag tggatgtccg agaccccgcg ctcgacgccg aacgcgagca
     4081 gcttgaggaa ggcgttgcgg tccatgaacc gatcagttca ggcccggccg tccgccaccg
     4141 ccttcatcgt cgggcacggt atcggggacg gtctgggcgc gccgcaggct gtcgagcagc
     4201 ggctcggcct ccggggtcgc gccgaggtcg tcgtcggtca gcggccgcca ggcggccgcg
     4261 accggctcgc cgcgcaccgg gtgcatgccg gagctctcgg gagacggatc ctcgtgcggg
     4321 atctggaagt ccttggtgat ctcgtcctcg gaccggccga acgcgttgcg gaccagcgcg
     4381 tcggccagca tgcgccggct cgagccgatc gcgagcggcc cgtcgcggtc gtcgtcgtcg
     4441 tcctcgtcgt cggtcgcgac cagcgcgacg tcgggcaccg ccgaggtcac gttgcgcagc
     4501 tcgccgcgca cgtcatcggg cacggtcggg aagccctggg ccggccgcgc catgtagtaa
     4561 ccctggccgt agcgcaggcc gaggtcgcgc agcgcgacga cgtcgtcgag ccgctcgatg
     4621 ccctcggcga tcatcaccgc gtcgatggtc tcggcgatgt ggcgcagcga gcgcagcatc
     4681 tcgcgcttga ccgtcgagcg cgcgatgccg cggatcagca cgtcgctgat cttgatgaag
     4741 tgcgggcgca gcgacatcac ggtctcgagg ttggagtgcc gggtgccgac gtcgtcgatc
     4801 gcgac
//
LOCUS       DASVOR010000370         5233 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_411989,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000370 DASVOR010000000
VERSION     DASVOR010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5233
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411989"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(630..1067)
                     /locus_tag="VF516_07565"
     CDS             complement(630..1067)
                     /locus_tag="VF516_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107571.1"
                     /translation="MPADASRRPPPRTPPRPDRIRRIAGGFAFVPNEFLHQGFFASLS
                     HAERSLYFFLVLASDRNGVSFYAHDRICDVLELTLDDYVLVRNRLIDNDLIAFDGRRF
                     QVLSLPPAPIAPARRPLVTQDDFEDHDPATIRHLIRSSLDRRR"
     gene            complement(1069..1839)
                     /gene="istB"
                     /locus_tag="VF516_07570"
     CDS             complement(1069..1839)
                     /gene="istB"
                     /locus_tag="VF516_07570"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX8107572.1"
                     /translation="MTRTLDQLDELLAWAAHERPGATALFEHVLGLEAAHKLEQRITR
                     RIDTSGLVERKALEGFDWEFQPTLDKALVLELARLDFVRRHDDLVITGDNGTGKSHLL
                     KAFALRACEQGIPMRYARCVDLLEDLHAGLADGTYARRLKGWARPAFLIIDDVGLGQV
                     KRRADEPTAAHTLYNLVDRRHGHVSTAVTSNIALSDWGKYLGDATLTAAILDRLAMHA
                     IRIDIAGPSYRQHVAHERAAGRGPKRSEHARPSAADEG"
     gene            complement(1836..3506)
                     /gene="istA"
                     /locus_tag="VF516_07575"
     CDS             complement(1836..3506)
                     /gene="istA"
                     /locus_tag="VF516_07575"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX8107573.1"
                     /translation="MTATWRSREELAHQVALLAKQGESRRAIARAVGVSRNTVRTLLA
                     AHAQDRNTEHVAIAPRPARAPRATKLDRWRPRVAALMAKFADITAQRVFEILRAEGFD
                     GGYTGVKRHVRAMRPPPKPTPSLTTPDYGPGEMSESDWSPYEIQFTTGKTCVIQALSY
                     VLVVSKRKYFGLYESNDLHALMDGTDRAFARFGGCAHESKYDSQKPVVLRWECNQPIY
                     NPRFLAFSSHYEFRPVAVRRGHPNDKPRVERSFWEVERSFLNGREFRDLDDMRAQLAH
                     WLEHIVDHRKLEKRTALERFAEEREHLVALPRHPYDTARVIYRVCSIDGFVAWAGNHY
                     AVPYDHVTGILPVRITQRELFVYAADLTCVARHELGPRGAGLRLDPAGLHPAPQRRSP
                     VDLDQLKIAFGNMGEHAAEFFGLMSLGPPRVWSAQARQILLLRERYATDEIDRALGHA
                     AAFGARDHATVERILAARALPRTLDEYVAEDTARRLEATFGHVRTVPRDLTEYDRLPL
                     TTASRSRAQETPAWPSEPTRPDPNSETAEPATTTPSSRDCDDTSTSSD"
     gene            complement(3503..4411)
                     /locus_tag="VF516_07580"
     CDS             complement(3503..4411)
                     /locus_tag="VF516_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX8107574.1"
                     /translation="MTTHHEPPQTASIAELGERLAALRLCEAAALEAMRQSLDRHGQL
                     MPIVAFAEGDHIETLDGFKRLRAARALGWATVAITLVEVADDVTAKVRLFELHHRRGL
                     TELEEAWLIRSLYRDDRLPAPEIARRMNRHRSWVWRRLMLVEALDPGVQTDVRLGLIA
                     PRAAIAVSRLPRGNQQAASAVVVRRGLTFRQAEALVDDALGLPDDAARAALLMRRLDG
                     PAISTPPGPRPFRATRSDADWMTLDIVRVRDIAARLEARLLSTPLDGFAPAVAELLRD
                     ELARLSPVLRALDGVITKVVGQAEVL"
     gene            complement(5050..>5233)
                     /locus_tag="VF516_07585"
     CDS             complement(5050..>5233)
                     /locus_tag="VF516_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107575.1"
                     /translation="LSKLESKKIRLQVLELVNALEREALDVFRLARRLERKLWNDPAR
                     LKSHGRAEIIQPSAAG"
BASE COUNT          750 a         1751 c         1903 g          829 t
ORIGIN      
        1 gcacgcgcgg cgtctaccgc gtgaacctgc gccacggcgt cagccgggtg cgctcgggac
       61 agcgccacac gctcggcgtc atcttccacg acgccgcgtg atcgacctcg cgaatggcct
      121 cgtcaggacg gccctgcatt ccggcagcta cgcgcgagca gtccctcgag cgagccgcat
      181 cgatcttgca caccctcgac acagaaggcc gccttgcacc ggcggatcgc gcgaagatcg
      241 atggggtcac aggatgctcg ctgccctgcc atgatccgcg gctctcacgg cgccacggcg
      301 gctccaggca gcggatgtcc aaaggccaga ggtgcgtgac gggcgttcgg ggagacctgt
      361 taaccgccgc cccgatggtt catagctccc ggtctgaccg acccaatggt taacgggacc
      421 tgggctttgg cggcgccttt ggattgatcc aatttcggcg gcggcatgga tccaccggac
      481 gagggtggtc gcggcgagat cgtgatccac tggatccgga tccgtgtgga tcccggtcgg
      541 gtataacccg ttcgtctgga tccctgattg tggatcggat ggatcttctg gatcgccgtg
      601 agggccgtag cgcgacgggc gcgttgcggt cagcgacggc gatcgagcga tgaccggatg
      661 aggtgccgga tggtcgctgg gtcgtggtcc tcgaagtcgt cctgagtgac cagcggccgc
      721 cgcgccggcg cgatgggcgc cggtgggagc gataggacct gaaaccggcg gccgtcgaag
      781 gcgatgaggt cgttgtcgat caagcggttg cgcacgagga catagtcgtc cagggtgagc
      841 tcgagcacgt cgcagatccg gtcgtgggcg taaaagctga cgccattgcg gtcactggcc
      901 aggacgagga agaagtagag cgagcgctcg gcgtggctca gggacgcgaa gaagccttgg
      961 tgcaagaact cgttggggac gaaggcaaag ccacccgcga tgcggcggat ccggtctggc
     1021 cgcggtgggg tcctgggtgg cggccttcgg ctggcgtcgg cgggcatgtc agccttcgtc
     1081 ggcagcgctg ggccgggcgt gctcgctccg cttggggcca cggccggcgg cgcgctcgtg
     1141 ggcgacgtgc tggcgatagc tcgggcccgc gatgtcgatc cggatggcgt gcatggcgag
     1201 gcgatcgagg atcgcggcgg tgagcgtggc gtcgccgagg tacttgcccc agtcgctgag
     1261 cgcgatgttc gaggtgaccg cggtggagac gtggccgtgc cggcgatcga cgaggttata
     1321 caacgtgtgc gcggcggtcg gctcgtcggc acggcgcttg acctggccaa ggccgacgtc
     1381 atcgatgatc aagaatgcgg gacgcgccca gcccttgagg cgccgggcat aggtgccgtc
     1441 ggcgaggccg gcgtggaggt cctcgaggag gtcgacgcag cgtgcgtagc gcatggggat
     1501 cccctgctcg caagcgcgca gggcgaaggc cttgaggagg tgactcttgc cggtgccgtt
     1561 gtcgccggtg atcacgaggt catcgtggcg gcgcacgaag tcgaggcgtg cgagctcaag
     1621 cacgagcgcc ttgtcgaggg tgggctggaa ctcccaatcg aacccctcga gtgctttgcg
     1681 ctcgacgagg ccgctggtgt cgatgcggcg tgtgattcgc tgctcgagct tgtgggcagc
     1741 ctcgaggccc aggacgtgct cgaacagcgc cgtggcaccg gggcgctcgt gcgctgccca
     1801 cgcgagcaac tcgtcgagct ggtcgagcgt ccgggtcaat ccgaggaagt cgaggtgtcg
     1861 tcgcagtcgc gcgatgacgg ggtcgtcgtc gctggctccg cggtctcgct gtttgggtct
     1921 ggtcgggtcg gttcgcttgg ccatgctggg gtttcctggg cgcgtgaccg cgacgcggtg
     1981 gtgaggggga ggcggtcgta ctcggtgagg tcacgcggca ccgtgcggac gtgaccgaag
     2041 gtcgcctcga gccggcgggc ggtatcctcg gcgacgtact cgtcgagcgt gcgcggcagg
     2101 gcgcgtgccg cgaggatgcg ctcgacggtg gcgtggtcgc gcgcgccgaa cgccgcagcg
     2161 tgaccgagcg cgcgatcgat ctcgtcagtg gcatagcgct cgcgcaggag caagatctgg
     2221 cgcgcctgcg cgctccagac ccgcggcggg ccgaggctca tcagtccgaa gaactcggca
     2281 gcgtgctcgc ccatgttgcc aaaggcgatc ttgagctggt cgagatcgac ggggctccgc
     2341 cgctgcggcg cggggtgcag cccggcgggg tcgagccgga ggcccgcgcc gcgcgggccg
     2401 agctcgtggc gcgcgacgca cgtcaggtcg gcggcgtaca cgaacagctc gcgctgcgtg
     2461 atgcgcacgg gcaggatgcc cgtgacgtgg tcatagggga cggcgtagtg attgccggcc
     2521 cacgcgacga agccgtcgat gctgcacacg cgatagatca cgcgcgcggt gtcgtacgga
     2581 tgccgcggca gcgcgaccag gtgctcgcgc tcctcggcga agcgctcgag cgcggtgcgc
     2641 ttttccagct tgcggtggtc gacgatatgc tcgagccagt gcgcgagttg cgcgcgcatg
     2701 tcgtcgaggt cgcgaaactc gcggccgttg aggaacgacc gctcgacttc ccagaacgac
     2761 cgctccaccc gtggtttgtc gttggggtga ccgcgccgaa cggccaccgg ccgaaattcg
     2821 tagtggctcg agaacgcgag gaaccgcgga ttgtagatcg gctgattgca ctcccagcgc
     2881 agcacgactg gcttctggct gtcgtacttg ctctcgtgcg cgcagccgcc gaatcgtgcg
     2941 aacgcgcggt cggtcccatc catcagcgcg tgcaggtcat tggactcgta gagcccgaag
     3001 tacttccgct tgctcacgac cagcacgtac gacagggcct ggatcacaca ggtcttgccg
     3061 gtggtgaact ggatctcgta gggcgaccag tcgctctccg acatctcgcc cggcccgtag
     3121 tcgggcgtgg tcaggctcgg ggtcggcttc ggcggcgggc gcatcgcccg cacgtgccgc
     3181 ttcacgccgg tgtagccacc gtcgaagccc tcggcgcgca ggatctcgaa cacgcgctgc
     3241 gcggtgatgt cggcgaactt cgccatcagc gcggcgaccc gcggtcgcca ccggtcgagc
     3301 ttcgtggcgc gcggcgcgcg ggccgggcgt ggcgcgatcg cgacgtgctc ggtgttgcgg
     3361 tcctgggcgt gggccgcgag cagcgtgcgc acggtgttgc ggctcacgcc gaccgcgcgg
     3421 gcgatcgcac ggcgcgactc gccctgcttg gcaaggagcg cgacctgatg cgcgagctcc
     3481 tcgcggctgc gccatgtcgc ggtcacaaga cctccgcctg cccgacgacc ttggtgatga
     3541 cgccgtcgag cgcgcggagc accggcgaca ggcgcgcgag ctcgtcgcgc aatagctcgg
     3601 ccaccgccgg cgcgaacccg tcgagcggcg tcgacaacag gcgcgcttcg agccgcgctg
     3661 cgatgtcacg cacgcgcacg atgtccaggg tcatccagtc ggcgtcgcta cgcgtcgcgc
     3721 ggaacggacg cggcccgggc ggcgtgctga tcgcggggcc gtcgagccgg cgcatgagca
     3781 gtgccgcgcg cgcggcgtcg tctggcagcc cgagggcgtc gtcgaccagc gcctcggcct
     3841 gccggaatgt caggccgcgg cgcaccacca cggcactcgc tgcctgctgg ttgccacgtg
     3901 gcaaccggct caccgcgatc gccgcgcgcg gcgcgatcag cccgagccgc acatcggtct
     3961 gcacccctgg atcgagcgcc tcgaccagca tcagccggcg ccagacccag ctccggtgcc
     4021 gattcatccg ccgcgcaatc tcgggcgcgg gcagtcgatc gtcccggtac agcgaacgga
     4081 tcagccatgc ctcctcgagc tcggtcagcc cgcgccgatg gtgcagctcg aacagtcgca
     4141 ccttggcggt gacatcgtcg gcgacctcga cgagcgtgat cgccaccgtc gcccagccca
     4201 gcgcgcgcgc cgcgcgcagg cgcttgaagc cgtcgagcgt ctcgatgtga tcgccctcgg
     4261 cgaacgcgac gatcggcatg agctgcccgt ggcgatcgag cgactggcgc atcgcctcga
     4321 gcgcggccgc ctcgcacaga cggagcgccg cgagtcgctc acccagctcg gcgatgctcg
     4381 ccgtctgcgg tggctcgtgg tgcgtggtca cggccgtcat ccgagcacgc gaggccctcg
     4441 cgacccgaag ccggcgcgtg atgcgcgccc aatttcggca cgctttccat cgtgattacg
     4501 gtggcttggg atgccaagcc ggcgcgtgac agccacccgt gtccgcccag atttcgccca
     4561 attccctcag gatctcgcgc actccgcggt cgaagccggc gcgtgacacg cgcgcggccc
     4621 tgtccacgat ctggcgcact ttgtcctgca actccgccga gatcggcgcc gaagccggcg
     4681 cgtgacatcc ggacgcctca ccccgccgct ggtcgcggtg cacctcctgc cgcgcgcgcg
     4741 cagcggcgcg gtagcccgct gggtcctggc gtcgtcgcac ccgcgcctgc ctccgtcgcc
     4801 tcgccagccg gcacgccggc ccgcacgtct tctgtcgatg gcccgtcgtc gccgcgggag
     4861 tgaaccgctt ccgacattcc gtgcatcgcg accgcttcgc catggggccg cagggtcacc
     4921 gaccgggtcc acctactggt gggtggacgg cgcatccaac cccgctgcca ggtggatcac
     4981 gaaagcgccg cggcggccgc cgccaaagcg gcgcactgga tcacgaaccc gccgcgaagt
     5041 ggatctgcct tagcccgccg ctgaaggctg gatgatctcg gctcgcccgt gactcttgag
     5101 ccgcgccgga tcgttccaca gcttgcgctc aaggcgccgg gcgaggcgga agacgtccag
     5161 tgcctcgcgc tctagggcgt tgacgagctc gagcacctgc agccggatct tcttgctctc
     5221 cagcttggac agc
//
LOCUS       DASVOR010000371         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_354203,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000371 DASVOR010000000
VERSION     DASVOR010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354203"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VF516_07590"
     CDS             <1..341
                     /locus_tag="VF516_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107576.1"
                     /translation="VTLSTSTALTAVESGKVALACAYTGQVSEATADSVVLVTARLPD
                     DELYLDLLARKERGELISARAIGDAWAPGTIAAAVWSGRRFAEEFDADLPDDVVPFRR
                     EITELYSDPA"
     gene            390..1856
                     /locus_tag="VF516_07595"
     CDS             390..1856
                     /locus_tag="VF516_07595"
                     /inference="COORDINATES: protein motif:HMM:NF023222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase family
                     protein"
                     /protein_id="HEX8107577.1"
                     /translation="MIRTGKEYKDSLADGREVWIDGERVQDVTSHPAFKPIVDVRARI
                     YDMAHDPATAATMTYTDDETGETCAIGGKPPRTKQDWHAKHAAVETVLDDVAGVVTRV
                     GDETVGEMWSLYDGQDVLNEIDPSFSANIRDHVRRAALLDPFHVSANTDPKGDRSRHP
                     RDQDPDVLLHVVRETDNGIVVRGAKFETAAAYANQAFVKPTIGDWGNDALSEYAVGFI
                     ADMGASGIRHICRSGFAGRLPAADYPLSNRFDEIDTMIVFDDVEIPWENVFFYRHTRA
                     AAFIRATLHRYSMYAFVHRHLRLADLLLGVAYANSKQTGVTMHQGVREKLAQLACYRE
                     GIAAHLAASIEMAQESPGGLLMPNQALLFTGRVHACSQLPDMMHLVRDICGCQFSLVP
                     SAAAFEAEGPGEWLAKFFRVGDIEAEDRRKVLVYARDLLNSDYAGHRLTFQLFAQSPP
                     FSHLLAVYNNYDFEPAVQRMAKSAGIEDPLGVRKESLA"
     gene            1853..2281
                     /locus_tag="VF516_07600"
     CDS             1853..2281
                     /locus_tag="VF516_07600"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX8107578.1"
                     /translation="MSESIIVGGHRRIRPFNTADTYPEQRISNDLCQAVVAGNTIYLR
                     GQVAQDLTTRESLAIGDAAGQAGKVMDNIELLLGEAGAGLEHIVTCDVYLTDIRHREQ
                     VYQALGERMRGVYPCFTGVVVQALARPEWLVEVTAVAVKP"
     gene            2278..>2942
                     /locus_tag="VF516_07605"
     CDS             2278..>2942
                     /locus_tag="VF516_07605"
                     /inference="COORDINATES: protein motif:HMM:NF018024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HEX8107579.1"
                     /translation="MTFSLAGRCARTGMLGVVVTSSSPAVAARCAWARAGVGAVATQN
                     ITDPSLGRQVLDRIAAGRSAAEALDEVMAATPHAAFRQLGVIDSCGRTAAYTGTRTLG
                     RHASEAGENCVAAGNLLADERVPAAMVSAFERSTQDHLVSRLLEALATGEAAGGEEGP
                     VRSCGLVVVGNVSWPITDLRVDWHTDPNGELALLWEIWRPLEHDYVLRATDPAAAPTY
                     GVPG"
BASE COUNT          493 a         1017 c          997 g          435 t
ORIGIN      
        1 gggtcacgct cagcaccagc acggcgctga ccgcggtcga gtccggcaag gtcgctctgg
       61 cctgcgccta caccggccaa gtcagcgagg ccactgccga cagcgtggtc ctggtgacgg
      121 ccaggctgcc cgacgacgag ctgtacctcg atctgctcgc caggaaagaa cggggcgagc
      181 tcatctccgc gcgcgcgatc ggcgacgcct gggcccccgg caccatcgcc gcggcggtct
      241 ggtcaggtcg ccgcttcgcc gaggagttcg acgccgacct gcccgatgac gtcgtgccgt
      301 tccggcgcga gatcaccgag ctctactctg acccggcatg aacactgaac cggcatgagc
      361 acggctacca ctaaccagaa ggacacgcag tgatcaggac aggcaaggag tacaaggact
      421 ccctggccga cggacgcgag gtgtggatcg acggcgagcg ggtccaggac gtcacgtccc
      481 atcccgcgtt caagccgatc gtcgacgtgc gagcccggat ctacgacatg gcgcacgacc
      541 cggcgaccgc tgcaaccatg acctacaccg acgacgagac cggcgagacc tgcgccatcg
      601 gcggcaagcc gccgcgcacc aagcaggact ggcatgccaa gcacgcagcg gtcgagacgg
      661 tcctcgatga cgtcgcgggc gtggtcaccc gggtcggcga cgagactgtc ggcgagatgt
      721 ggtccctgta tgacggccag gacgtgctga acgagatcga cccgagcttc tccgccaaca
      781 tccgcgacca tgtccgccgc gctgccttgc tagacccgtt ccacgtgtca gctaataccg
      841 accccaaggg tgatcggagc aggcacccgc gcgatcagga ccccgacgtg ctgctgcacg
      901 tcgtccgcga gacggacaac ggcatcgtgg tccgcggcgc gaaattcgag accgcggccg
      961 cctacgccaa ccaggccttc gtcaagccca cgatcggcga ctggggcaac gacgccctct
     1021 cggagtacgc cgtcggcttc atcgccgaca tgggagcgtc cgggatccgg cacatctgcc
     1081 ggtccggctt cgccggccgg ctgcccgcgg cggactaccc gctgagcaac cgcttcgacg
     1141 agatcgacac gatgatcgtc ttcgacgacg tcgagatccc ctgggagaac gtgtttttct
     1201 accggcacac cagggccgcc gccttcatcc gggccacgct gcaccggtac agcatgtatg
     1261 ccttcgtgca ccggcacctg cgcctggcgg acctcctgct cggcgtcgcg tacgccaact
     1321 ccaagcagac cggcgtgacg atgcaccagg gggtgcgcga gaagctcgcg cagctggcgt
     1381 gttaccgcga gggcatcgcc gcgcacctgg ccgcctcgat tgagatggcg caggagagcc
     1441 cgggcggctt gctgatgccg aaccaggcgc tgctcttcac cggccgggtg cacgcctgct
     1501 cccagctgcc ggacatgatg cacctggtca gggacatctg cggctgccag ttcagcctgg
     1561 tgccgagcgc tgccgctttc gaggcggaag ggccaggcga atggctggcg aagttcttca
     1621 gggtcggtga catcgaggcg gaggaccggc gcaaggtcct cgtctacgcc cgcgacctgc
     1681 tgaactccga ctacgccggg caccggctga cgttccagct gttcgctcag tcgccgccct
     1741 tctcgcacct gctggccgtc tacaacaact acgacttcga acctgccgtg cagcggatgg
     1801 cgaagtcggc aggcatcgag gatccgctcg gcgttcggaa ggagtcgctg gcgtgagcga
     1861 gtcgatcatc gttggcgggc accgcaggat ccggccgttc aacaccgcgg acacctatcc
     1921 cgagcagcgc atctccaacg acctctgcca ggcggtggtg gctggcaaca cgatctatct
     1981 gcgcgggcag gtggcgcagg atctgaccac gcgggagtcg ctcgcgatcg gcgatgcggc
     2041 tggccaggca ggcaaggtga tggacaacat cgagctgctc ctcggcgaag ccggcgccgg
     2101 gctggaacac atcgtcacct gcgacgtgta cctgaccgac atccggcacc gcgagcaggt
     2161 ctaccaggcg ctcggcgagc ggatgcgcgg cgtgtatccg tgcttcaccg gcgtcgtcgt
     2221 ccaggctctc gcgcggccgg aatggctggt ggaagtcacc gcggtcgcgg tcaagccatg
     2281 acgttctcgc tcgcgggcag gtgcgccagg accgggatgc tcggcgtggt ggtgacctcc
     2341 tcaagtcccg ccgtggccgc tcggtgcgcc tgggcgcgcg caggggtggg cgccgtggcc
     2401 acccagaaca tcaccgatcc ctcgctgggg cgccaagtcc tggaccgaat cgcggcgggg
     2461 cgatcggcgg ccgaagcgct cgatgaggtg atggcggcca cgccgcatgc cgcgttccgc
     2521 cagctaggcg tcatcgactc ctgcggcaga acggccgcat acacgggcac ccgcaccctc
     2581 ggccggcacg ccagcgaggc tggcgagaac tgcgtcgccg ccggcaacct gctggccgac
     2641 gagagggtgc ccgcggcgat ggtcagcgcc ttcgagcgga gcactcagga ccacctcgtt
     2701 agccggctgc tcgaggcgct tgcgaccggc gaggcggccg ggggcgagga aggcccggtg
     2761 cgctcttgtg gcttggtggt cgtcggcaac gtgagctggc ccatcaccga cctgcgggtc
     2821 gactggcata cggacccgaa cggcgagctc gccctgttgt gggaaatatg gcgcccgctg
     2881 gaacacgact acgttcttcg cgccaccgat ccggcggccg cgcccactta cggagtgcca
     2941 gg
//
LOCUS       DASVOR010000372         2618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266316,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000372 DASVOR010000000
VERSION     DASVOR010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266316"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF516_07610"
     CDS             complement(<1..219)
                     /locus_tag="VF516_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107580.1"
                     /translation="MRAQYGSEFDRLLDRARTLLGEADDAVLGEAHTAVNQALTLCPQ
                     SVDGWLVKCQVASAAGDDIAALAAAEMAL"
     gene            complement(279..1400)
                     /locus_tag="VF516_07615"
     CDS             complement(279..1400)
                     /locus_tag="VF516_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8107581.1"
                     /translation="MTDDSDPPDPTGDDDWYGDADPRVPDASAWPDRDLRWLGDDVYL
                     GEQAQIAAEHPEPPAEATRDPAAEIRELERRLEQQRTPVFPIDLRRRLPLEGVWRRWR
                     ALAMWGRSDVVDDFGRDPRATARWEWLFEFLYSRWFRVEASGLDHIPSRGRALLVANH
                     AGSLPYDSAMVMHAVRRDHPARRDVRPLVEDTVFHLPFLGPVMNRIGGVRADPENAER
                     LLMKDELVAVFPEGEKGMGKLWKDRYRLQRFGRGGFVKLALRTGAPIIPVAVVGAEEA
                     APMLGKVTWFAKNIGIPWIPVTPIFPWLGPAGLLPLPSKWYVAFGPPIDLGKAHGPAA
                     AEDRLLVNKLADQIRTQIQDMIEQLLGQRRSPVLPAFLG"
     gene            complement(1441..2445)
                     /locus_tag="VF516_07620"
     CDS             complement(1441..2445)
                     /locus_tag="VF516_07620"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8107582.1"
                     /translation="MASSSTGRVVAVTGACTYLGGELLRRLEEDPRYARILALDIRPP
                     SLVTGGKTEFVKIDLTQPTIDGELATLLQRHAIDTFVHGAFLSHPTHASDWAHELEDV
                     GTMHVLNACAGVEPRRLVLISTTLVYGAHPKNPNFLTEDSELRGHRDSRFVNDKVRAE
                     RQVHRFAKEHPGVEVAILRFAPILGPTVSNMYTRFLSRPIAPVMMGHDPLMQFVHEQD
                     AAYALQRAVESHATGPFNIVGKGVLPYTTVLALLGRVPLPMPQLIARQLTKVLWTTQL
                     VGSPPSFLDFLLYLCVADGARARRELGFSPRLSIKRTILDFLGIAAEDGATDVATAQA
                     "
BASE COUNT          383 a          890 c          986 g          359 t
ORIGIN      
        1 cagcgccatc tcggcggcgg ccagcgcggc gatgtcgtcg ccggcggccg acgcgacctg
       61 gcacttgacc agccagccgt cgaccgactg cgggcagagg gtcagggcct ggttgacggc
      121 ggtgtgcgcc tcgccgagca ccgcgtcgtc ggcctcgccg agcagggtgc gcgcgcgatc
      181 gagcaggcgg tcgaactccg agccgtactg cgcacgcacg gcgtccacag taacacggcg
      241 cccgcggccg ggctaacgtg agccggcgct ggcggtggct accccaggaa cgcgggcagg
      301 accggcgagc ggcgctggcc gagcagctgc tcgatcatgt cctggatctg ggtccggatc
      361 tggtcggcga gcttgttgac cagcaggcga tcctcggcgg cggcggggcc gtgcgccttg
      421 ccgagatcga tcggcgggcc gaacgcgacg taccacttgc tcggcagggg caagaggccc
      481 gccgggccca gccacgggaa gatcggtgtc accgggatcc acgggatgcc gatgttcttg
      541 gcgaaccagg tcaccttgcc gagcatcggc gcggcctcct cggcgccgac caccgcgacc
      601 gggatgatcg gcgcgccggt gcgcagcgcg agcttgacga acccaccgcg gccgaaccgc
      661 tgcagccggt agcggtcctt ccacagcttg cccatgccct tctcgccctc ggggaacacc
      721 gcgaccagct cgtccttcat cagcaggcgc tcggcgttct cgggatcggc gcgcacgccg
      781 ccgatccggt tcatcaccgg cccgaggaac ggcaggtgga acacggtgtc ctcgaccagc
      841 gggcggacgt cgcggcgcgc ggggtgatcg cggcggaccg cgtgcatcac catcgcgctg
      901 tcgtagggca acgagcccgc gtggttggcg accagcagcg cgcggccccg ggacgggatg
      961 tgatcgaggc cggaggcctc gacccggaac caccgcgagt acaggaactc gaacagccac
     1021 tcccagcgcg cggtggcgcg cggatcgcgg ccgaagtcgt cgaccacgtc gctgcggccc
     1081 cacatcgcga gcgcgcgcca gcgccgccac acgccctcga gcggcagccg ccggcgcagg
     1141 tcgatcggga acaccggggt gcgctgctgc tccagccggc gctcgagctc gcggatctcg
     1201 gccgcggggt cgcgcgtcgc ctcggcgggc ggctcggggt gctcggcggc gatctgggcc
     1261 tgctcgccga ggtagacgtc gtcgccgagc caccgcaggt cgcgatccgg ccacgccgag
     1321 gcgtccggca cgcgcggatc ggcgtcgccg taccagtcgt cgtcgccggt ggggtccggt
     1381 gggtcggagt cgtcggtcat cggctcaccg tcgggatcat gcctgggaaa cccgcgtccg
     1441 tcacgcctgc gccgtcgcga cgtcggtcgc gccgtcctcg gcggcgatgc cgaggaaatc
     1501 gaggatcgtt cgcttgatcg acaggcgcgg cgagaacccg agctcgcggc gcgcccgggc
     1561 gccgtcggcg acgcacaggt agagcaggaa atcgaggaag ctcggcggcg acccgacgag
     1621 ctgcgtggtc cacagcacct tggtcagctg acgcgcgatc agctggggca tcggcagcgg
     1681 cacgcggccg agcagcgcga gcacggtggt gtagggcaag acgcccttgc cgacgatgtt
     1741 gaacggcccg gtggcgtgcg actcgacggc gcgctgcagc gcgtacgccg cgtcctgctc
     1801 gtggacgaac tgcatcagcg ggtcgtggcc catcatcacc ggggcgatcg gccgggacag
     1861 gaaccgggtg tacatgttcg acacggtcgg cccgaggatc ggcgcgaacc gcaggatcgc
     1921 gacctcgacg cccgggtgct ccttggcgaa ccgatggacc tggcgctcgg cgcggacctt
     1981 gtcgttgacg aaccgcgagt cgcggtggcc gcgcagctcg gagtcttcgg tcaggaagtt
     2041 ggggttcttg gggtgcgcgc cgtagaccag cgtggtcgag atcaggacga gccggcgcgg
     2101 ctcgacgccg gcgcacgcgt tgagcacgtg catcgtgccg acgtcctcga gctcgtgggc
     2161 ccagtccgag gcgtgggtcg ggtgagacag gaacgcgccg tggacgaacg tgtcgatcgc
     2221 gtggcgctgc agcagcgtgg cgagctcgcc gtcgatcgtc ggctgggtca ggtcgatctt
     2281 gacgaactcg gtctttcccc ccgtgaccag ggacggcggc cggatgtcga gcgccaggat
     2341 ccgcgcatat cggggatcct cttcgagccg gcgcaggagc tccccaccca ggtaggtgca
     2401 cgcacctgtg accgccacga cgcggccggt ggaggagctc gccatgcgcg ccttatagcg
     2461 cacccccggg gcagatcaga ggggtgtggt agcgatccgg tcgtggaacc gatgatcgcg
     2521 gtgttagccc cgcacgtgat cgatcagatc gcggccggcg aggtgatcga gcggccggcg
     2581 tcggtggtca aggagctggt cgacaacgcg atcgacgc
//
LOCUS       DASVOR010000373         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_383348,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000373 DASVOR010000000
VERSION     DASVOR010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383348"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..534)
                     /locus_tag="VF516_07625"
     CDS             complement(136..534)
                     /locus_tag="VF516_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8107583.1"
                     /translation="MIAVLDTHIMLWWFDRSRRLTRNQTRMLARNDADHPLGVADISL
                     WEVAMLVELGRVRLGLPIDEWLARATAAPRVEVQPVTPAIASEVIALGSTRDWDPADR
                     IIVATARVLGVPLVTSDTRISDSKLVPTIW"
     gene            complement(531..845)
                     /locus_tag="VF516_07630"
     CDS             complement(531..845)
                     /locus_tag="VF516_07630"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX8107584.1"
                     /translation="MKSVAISTFKARCLALLEDVARTGKPLLVTKRGKPLARLTAADH
                     SRSSSPQDTLRHSVETLGDVIAPTIPATAWDATRGVLLTRDGGSTVRTRRGRQTDRRS
                     RR"
     gene            1598..3112
                     /locus_tag="VF516_07635"
     CDS             1598..3112
                     /locus_tag="VF516_07635"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX8107585.1"
                     /translation="MPGDGSSREESLASERDFYRRLLDLGGKEEIEPLLDEALALIVE
                     VTGASTAYLELYDDEANRPRFWKGYRCTDRDVASIRASISRGIIARAIGEGRTIETPS
                     ASLDARFSELGSVRQNLIQAVLCAPVGVQPPIGVIYLQGRTRPGSFTAIDRERAELFA
                     RQLAPLADRLVRRDHHDHVDHTREVRERLRCPEIIGRSRALSQLLAAASHVAPLDVDV
                     LITGPSGTGKSMLARAIHLNSPRASGPFVELNCAAIPETLIENELFGAERGAHSTAMR
                     KVTGKVAAAEGGTLFLDEVAELPAGAQAKLLQLLETREYHSLGSTTSIRADVRILSAT
                     NAQLENRVATKQFREDLYYRIHVVPLEVPGLGERRDDIPELVEHFCVESCKRHRLPYL
                     AVSRRALAACRDSPWPGHTRQLAHAIEAAVIRASGERSTTLHEHHIFPKVSRDETEWV
                     TLQQATREFQKRHVRAALENNDWNVTETARQLDLARSHLYNLINDFELRREKKI"
     gene            3134..>3721
                     /locus_tag="VF516_07640"
     CDS             3134..>3721
                     /locus_tag="VF516_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107586.1"
                     /translation="MSKVTFYGLPAWVGAIHGPTVCDWWNHDPGQQQWDRSLLHRVDL
                     AKTPSAALAGQIPVSQVEADDHATKGGRTTIGPLFPGGTMIGALWVSESYLAANQLTA
                     PGHEFQLIQYFDGEQQNRRFYGFQASVLQTQNHLSLVQLWHPGTGAGTAPPAGTWWLD
                     LGASADPSAPPPSPGQAAPLYLDSKAASQLVTIGPK"
BASE COUNT          595 a         1278 c         1341 g          507 t
ORIGIN      
        1 cgcgccggcg gcccggctac cacgccggtg gcttgtggaa cggtcccttg gcgtcggtca
       61 gggtgtctgg aaccggctcg ccgagagcgc accgagccgg accggcttga gatctgcccc
      121 ccggccagtc cgtcactacc agatcgtcgg aacgagcttc gagtcgctga tccgggtatc
      181 ggacgtgacc aggggcactc cgagcacgcg cgcggtggcg acgatgatcc ggtcggcggg
      241 gtcccagtcc cgcgtcgagc cgagcgcgat cacctcgctt gcaatggcgg gcgtgactgg
      301 ctggacctcg acgcgcggag cagcggtcgc gcgcgcaagc cactcgtcga tcggaagccc
      361 gagccgtacc cggccgagct cgacgagcat cgcaacctcc cacagcgaga tatccgcgac
      421 cccgagcgga tgatcggcgt cgtttcgggc gagcatgcgc gtctggttgc gcgtcagacg
      481 ccgggaacgg tcgaaccacc agagcatgat gtgcgtgtcg agtaccgcga tcatcggcgg
      541 gatcggcgat ccgtctgtcg tccgcgccgc gtgcgcaccg tgctaccgcc gtcccgggtc
      601 agcagcactc ctcgggtggc atcccatgcg gtcgccggga tcgtcggagc gatgacgtca
      661 ccgagcgtct cgaccgagtg tcgcaaggtg tcctgggggg agctcgaccg gctgtggtcc
      721 gcagccgtga ggcgagcgag cggcttgccg cgcttggtga ccagcagcgg cttgcccgtg
      781 cgtgcgacgt cctccagcag cgcgagacac cgcgccttga aggtcgagat cgctaccgac
      841 ttcatggtca cctgagttga ccatatggtg cacgccggga caaggccgag ctacccgggg
      901 acgagctccc ctgaaaaagc tgcggcctcc cgagagaggc cgcgcaccac gccaccgaag
      961 tgacgtggga tgcgcctgaa ggtcatgggc tccggatcga cgacgcaagg atcgaccggc
     1021 ggcgccggcg gaccgcgccg cgcggtcagc gcgccgcgcc gcccgaggta ggcacggtgg
     1081 agcggagtgg aggcatcgga acgagcagcc accacgcgat cgcggcgagg aggacgatcg
     1141 acatgccctc gggtacagca cgggccgtgc ccgggtaacc tcgcgcggcg cggcgccgcc
     1201 acgcgcactc gatgcgccgg gccggcggac acctgcgcgc attgccggca ggttcgcgac
     1261 cggcccgcgc gcaagcgcgc cggacgagca ccgcgccgcg catgcgcatc gctcgcgtgc
     1321 gcggcgctat gtacccgttc accgcagcaa actgctgcgg catgatgcgg cagcgtcccg
     1381 tggatgccgc atgacacggc agctcagcag gccattccac cgttgggggc cgtacaagac
     1441 atcgggacag ccgctgcgag ggccgttcag ccgagcggac aatttgcggt gggaatgtgt
     1501 agcgcggaag gcgatgagcg ccaatggttg gaccaggtac ggtccgttgc catccaagcg
     1561 tcgatttcga ctcgatgtcg atcgatactg ccggggcatg cctggagacg gttcttcacg
     1621 tgaggagagc ctcgcgagcg agcgagactt ctaccggagg ctcctggacc tcggcggcaa
     1681 ggaggagatc gagccgctgc tcgacgaggc gctggcgctg atcgtggaag tcaccggggc
     1741 cagcacggcg tacctggagc tgtacgacga cgaggccaac cggccgcgat tctggaaagg
     1801 ctaccgctgc accgaccgcg acgtggcgtc gatccgggcg tcgatctcgc gcggcatcat
     1861 cgcccgcgcg atcggtgagg ggcggacgat cgagacgccg tcggcgtcgc tcgacgcgcg
     1921 gttctcggag ctcggcagcg tccggcagaa cctgatccag gcggtgctgt gcgcgccggt
     1981 cggggtgcag ccgccgatcg gggtgatcta tctgcagggc cggacgcggc cggggtcgtt
     2041 cacggcgatc gaccgcgagc gcgcggagct gttcgcgcgc cagctcgcgc cgctggccga
     2101 ccggctggtg cgccgcgatc accacgacca cgtcgaccac acgcgcgagg tgcgcgagcg
     2161 gctgcgctgc ccggagatca tcgggcgctc gcgggcgctg tcgcagctcc tggccgcggc
     2221 gtcgcacgtc gcgccgctcg acgtcgacgt gctgatcacc gggccgtccg ggaccggcaa
     2281 gtcgatgctg gcgcgcgcga tccacctgaa cagcccgcgg gcgtcggggc cgttcgtcga
     2341 gctcaactgc gcggcgatcc ccgagacgct gatcgagaac gagctgttcg gcgccgagcg
     2401 cggcgcgcac tcgacggcga tgcgcaaggt gaccggcaag gtggcggcgg ccgagggcgg
     2461 cacgctgttc ctcgacgagg tcgccgagct gccggccggc gcgcaggcca agctgctgca
     2521 gctgctggag acccgcgagt accactcgct gggctcgacg acgtcgatcc gcgccgacgt
     2581 ccggatcctg agcgcgacca acgcgcagct cgagaaccgg gtcgcgacca agcagttccg
     2641 cgaggacctg tactaccgga tccacgtcgt gccgctcgag gtgcccgggc tgggcgagcg
     2701 gcgcgacgac atccccgagc tggtcgagca cttctgcgtc gagtcgtgca agcggcaccg
     2761 gctgccgtac ctcgcggtgt cgcgccgcgc gctggcggcg tgccgcgact cgccgtggcc
     2821 cggacacacc cggcagctcg cgcacgcgat cgaggccgcg gtgatccgcg cgagcggcga
     2881 gcgctcgacg acgctgcacg agcaccacat cttccccaag gtgtcgcgcg acgagaccga
     2941 gtgggtgacg ctgcagcagg cgacgcgcga gttccagaag cgccacgtcc gcgccgcgct
     3001 ggagaacaac gactggaacg tgaccgagac cgcgcgccag ctcgacctcg cgcgctcgca
     3061 tctctataac ctgatcaacg acttcgagct tcgacgcgaa aagaaaatct gattcctgaa
     3121 ggggggcgta ctgatgagca aggtgacatt ctatggattg cccgcctggg tcggcgcgat
     3181 ccacggcccg acggtgtgtg actggtggaa ccacgatccg ggccagcagc agtgggaccg
     3241 ctcgctgctc catcgcgtgg acctcgcgaa gacaccgtcg gccgcgctcg ccgggcagat
     3301 cccggtctcg caggtcgagg ccgacgacca cgcgaccaag ggcgggcgga cgacgatcgg
     3361 gccactgttt ccgggaggga cgatgatcgg cgcgctctgg gtgtccgaga gctacctggc
     3421 ggccaaccag ctgaccgcgc ccggccacga gttccagctg atccagtact tcgacggcga
     3481 gcagcagaac cgccggttct acgggttcca ggccagcgtg ctgcagaccc agaaccacct
     3541 gtcgctggtc cagctctggc acccggggac cggcgccggc accgcgccgc cggccgggac
     3601 gtggtggctc gatctgggcg ccagcgccga tccgagcgcg ccgccgccca gcccgggcca
     3661 ggcggcgcca ctgtacctcg actccaaggc ggcgagccag ctggtcacga tcggcccgaa
     3721 g
//
LOCUS       DASVOR010000374         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_354226,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000374 DASVOR010000000
VERSION     DASVOR010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354226"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF516_07645"
     CDS             complement(<1..325)
                     /locus_tag="VF516_07645"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEX8107587.1"
                     /translation="MDTGDTGDTGDAGDAANAGAGANGSQGPALDPAARQSRRMYEGL
                     SASAAGLELGISVAIGALFGAWLDGKLGTAPWLLIGFLIIGVIAGFRGVLRAVARAER
                     ADRAER"
     gene            complement(434..1708)
                     /gene="hemL"
                     /locus_tag="VF516_07650"
     CDS             complement(434..1708)
                     /gene="hemL"
                     /locus_tag="VF516_07650"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX8107588.1"
                     /translation="MPIQRSQSIFERARAVIPGGVNSPVRACRSVGADPVFIASGRGG
                     RVTDADGNEYLDLIGSWGPLILGHCHPEIMAAIQGAMAHGTTFGAPTEIEVAFAEALR
                     AAVPTMEMVRAVSSGTEATMSALRLARGFTGRPRIIKADGGYHGHADCLLVAAGSGAA
                     TLGIPGSAGVPDGAARDTIVVPYNDLGSVERALAAGDVAAVIIEPVAGNMGLVAPAPG
                     YLEGLRALTARHGTVLIFDEVMTGFRVAYGGAQARYGVAPDLTTIGKIIGGGLPAAAF
                     GGRADIMQKLAPLGPVYQAGTLSGNPLAMAAGLKAMEILGRPGTYERLEQLGQRLGDG
                     LLAAARAAGVPGVMNRVGSMLTLFFCNGPVTDYASAKTADTARFGAFFRNMRDRGVFL
                     PPSQFEAMFVSLAHSDEDIDQIVAAARAALAN"
BASE COUNT          252 a          713 c          667 g          251 t
ORIGIN      
        1 tccgctcggc ccggtccgcg cgctcggccc gcgcgaccgc gcgcagcacg ccgcggaacc
       61 cggcgatcac gccgatgatc aggaacccga tcaggagcca cggcgccgtg ccgagcttgc
      121 cgtcgagcca ggccccgaac agcgcaccga tggccaccga gatcccgagc tccaggccgg
      181 ccgcgctcgc cgacagcccc tcgtacatcc gccgggactg ccgcgccgcg ggatccaggg
      241 cagggccctg cgaaccgttc gcgccagcgc ccgcgttggc ggcatcgccg gcatccccgg
      301 tatccccggt atccccggta tccatgggcg ttcgatgcac gcgctcgtgc gccgaatcct
      361 gcgacatttc gccgctatga cggggcccgg actgcatcga cgcggcccct catagcaccc
      421 ggtttttgca aggtcaattg gccagcgccg ctcgtgcggc ggcgacgatc tgatcgatgt
      481 cctcgtcgct gtgcgccagg ctgacgaaca tcgcctcgaa ctgcgagggt ggcagaaaca
      541 ccccgcgatc ccgcatgttg cggaagaacg cgccgaaccg cgcggtgtcg gcggtcttcg
      601 cactcgcgta gtcggtgacc ggaccgttgc aaaagaacag cgtgagcatc gagccaacgc
      661 ggttcatcac ccccggcacc ccggccgccc gggccgctgc gagcaggccg tcgcccaggc
      721 gctggccgag ctgctccagt cgctcgtagg tccccggccg gcccaggatc tccatcgcct
      781 tgagcccggc cgccatcgcc agcggattgc cgctcagcgt gccggcctgg tagaccggcc
      841 cgagcggcgc gagcttctgc atgatgtcgg cgcggccgcc gaacgccgcg gccggcagcc
      901 cgccgccgat gatcttgccg atcgtcgtga ggtccggcgc gacgccgtag cgcgcctggg
      961 cgccgccgta ggcgacccgg aacccggtca tcacctcgtc gaagatcagc acggtgccgt
     1021 gcctggcggt cagcgcgcgc aggccctcga ggtagccggg cgcgggcgcg accagcccca
     1081 tgttgccggc gaccggctcg atgatgaccg ccgcgacgtc gccggcggcc agcgcgcgct
     1141 cgacgctgcc caggtcgttg tacggcacga cgatcgtgtc gcgggccgca ccgtcgggga
     1201 cgcccgccga gcccgggatg cccagggtgg cggcgcccga gccggcggcg accagcaggc
     1261 agtcggcgtg gccgtggtag ccgccgtcgg ccttgatgat ccgcggccgg ccggtgaacc
     1321 cgcgggcgag ccgcagcgcg ctcatcgtcg cctcggtgcc cgacgacacc gcgcgcacca
     1381 tctccatggt cggcaccgcg gcgcgcagcg cctcggcgaa cgcgacctcg atctcggtcg
     1441 gcgccccgaa cgtcgtgccg tgcgccatcg cgccctggat cgccgccatg atctcggggt
     1501 ggcagtggcc gaggatcagc ggcccccagc tgccgatcag gtcgaggtac tcgttgccgt
     1561 cggcgtcggt gacccggccg ccgcggccgc tggcgatgaa caccgggtcc gccccgaccg
     1621 agcggcacgc ccggaccggc gagttgacgc ctcccgggat gacggcgcgg gctcgctcga
     1681 agatcgactg gctgcgttgg atcggcatcg cgcggaacgt accgcggatc gcacaggcgg
     1741 tggcgccggt gctccatcgg tcacgccagg tggcacgcgc ggagcgcggg tggtgcgacc
     1801 ctgtggtgtt ccggcgtcgc aacccggccc ggtcgcgtcg aggcctcgcg cgccggcacc
     1861 ggagagtgta ctttggctgc atg
//
LOCUS       DASVOR010000375         3867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_61966,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000375 DASVOR010000000
VERSION     DASVOR010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61966"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..739
                     /locus_tag="VF516_07655"
     CDS             <1..739
                     /locus_tag="VF516_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107589.1"
                     /translation="ASPGYPSPGYPPPGYPPPGYPMPAAPGYPMPAPPGYPPPFGAGA
                     MGQPQAGFDALGALWQALRQGQIPPLPGQLPGGMGAGQLAPPPRATMPGARPPADATA
                     LLGMFLGGLPQLSGALRSAAVLGPDGPRTMALSIPAAGAPGGVRNVQIPLGAVMNTLA
                     SLASRSMEELNASTQESDPEIPDYLVDEEGRFLVDPASAAARAGLVTHIVRVHQAAHR
                     AGWFAEAEEGLDEAEAWARDAGFERWE"
     gene            742..1248
                     /locus_tag="VF516_07660"
     CDS             742..1248
                     /locus_tag="VF516_07660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107590.1"
                     /translation="MKADTTKAMQDALDAAVRSLNNGSATEAPSRSSDTFGAIMTLLP
                     RLLRSNETGEDLVEKLDKLQKGDIAALKDQVLTLRKQCHRLLRTQELVMAQLAEIDKR
                     QSMMIDAILDLRDQMARVVLVEDPPDGDGVETRQPAADQGTQGRGEPQSGPRKRPQHK
                     AAKNRART"
     gene            1272..2639
                     /locus_tag="VF516_07665"
     CDS             1272..2639
                     /locus_tag="VF516_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107591.1"
                     /translation="MSESANFEIDEDAEDESFAAEGDGEDTEDTEDTEDTEDTEDAEA
                     FAAEADGEDTEDTEDTEDTEDAEDAEAMSASDRLSARIAADRDRERRARWQAQLARDR
                     QREAQAAGAVQQSLTSRLQGIRGSGRTGTVTLGNINGVRRIEMTLANGRKAVVQVVPP
                     LASANEVNRLRAVVNTNEKRRANSDAANSRAISRLASAQVAFVKRQTAQQVKSDRELG
                     KRIVEGYSRLDKRITTEIANQKANVGKGDKRIFRRLKRVRYQLLMNDVLLASALPFFA
                     AYGKQGSPFDKRNLALTGVLAGWLLGDELIGLATGMNKSSKAMNTLQTTWATVAPIGN
                     GLTDFLLYRNTQNSRFLTGTTTIPVPTAGSPTPNQVTVTVPIAVSSASTFNTLKNPPV
                     VATVVSGHVQTGSPLGPITPTIQASIDTGKLTLVVGEGTITEAIVVAWVIDTDPGVNP
                     THVAV"
     gene            2677..3852
                     /locus_tag="VF516_07670"
     CDS             2677..3852
                     /locus_tag="VF516_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107592.1"
                     /translation="MPIRPKLRRTAREAVAEDYLDVASRRPGGDPVGRAPPVRGTPLV
                     TGFLANGRVVPLSVREPLAPLGDLDQLVGTLRRMGDDADAAIRARSQAIVRLSRTVGS
                     DAAQLGRESGAARRKLERQALAASARLDRRIDKELIPLREDIAKAGARHERLVRRLAR
                     RALWDDLVVLSALPLFAAFGQRDNPLHSSNLALTLSLLVWLLGDDLTDLISGAETVFG
                     IRDTDIWSYIAPLANVLTGWFLLRNAQNERFITGFASDFRLVFVTADALLSAKAGVGK
                     PAGGTADTGLSERLFKFRQQIDLSLFVAPDHFDAFLGFEHIPAVASIAQITWGPGLDD
                     PAARRIVGLTAVAEDGVLTITITITVAVSYLKQLPRPEAVLSALRAAWIVDTAQPKA"
BASE COUNT          644 a         1342 c         1388 g          493 t
ORIGIN      
        1 ggcgtcgccg gggtatccgt cgccggggta tccgccgccg gggtatccgc cgccggggta
       61 tccgatgccg gcggcgccgg ggtatccgat gccggcgccg ccggggtatc cgccgccgtt
      121 cggcgccgga gcgatgggtc agccgcaggc cggcttcgat gcgctgggag cgctgtggca
      181 ggcgctgcgc cagggacaga tcccgccatt gccgggccag ctgccgggtg gcatgggggc
      241 aggccagctg gcgccaccgc cgagggcgac gatgccgggg gcgcgccctc ccgcggacgc
      301 gaccgcgctg ctggggatgt tcctgggggg gctgccgcag ctttcgggcg cgctgcggtc
      361 ggcggccgtg ctcggtccgg atgggccgcg cacgatggcg ctgtccatcc cggccgccgg
      421 cgcgcccggt ggtgtgcgca acgtgcagat cccgctcggc gcggtgatga acaccctcgc
      481 ctccctggcc agtcgctcga tggaggagct gaacgccagc acgcaggaga gcgaccccga
      541 gataccggac tacctggtcg acgaggaggg aaggttcctc gtcgatccgg ccagcgccgc
      601 ggcgcgggcg ggtctggtga cccacatcgt tcgcgtgcac caggccgcgc accgcgccgg
      661 gtggttcgcc gaggcagaag aaggcctgga cgaggcggag gcctgggccc gcgacgcggg
      721 gttcgaacgc tgggagtaac cgtgaaagca gacacgacca aagccatgca ggacgcgctg
      781 gacgcggccg tgcgaagcct gaacaacggc agcgcaacgg aggcgccgtc gcgatcctcc
      841 gacacgttcg gagccatcat gacgctgctt cccagactgc tgcgcagcaa cgagacgggc
      901 gaggatctgg tggagaagct ggacaagctg caaaagggcg acatcgcggc cctcaaggat
      961 caggtgctga ccctgcgcaa gcagtgtcat cgcctgctca ggacgcagga gctggtgatg
     1021 gcccagctgg cggagatcga caagcgccag agcatgatga tcgacgctat cctggacctg
     1081 cgggaccaga tggcgcgcgt cgtcctcgtg gaggatccgc cggacggaga cggcgtggag
     1141 acgcgccagc cggcggcgga tcaaggcact cagggccgcg gcgagccgca gagcggcccg
     1201 aggaaacgcc cgcagcacaa ggcggccaag aacagggcaa ggacttagaa ccgatcaggg
     1261 aggtcacgat gatgagcgag agcgcgaatt tcgagatcga cgaggacgcc gaggacgagt
     1321 ccttcgccgc cgagggcgac ggggaggaca ccgaggatac cgaggacacc gaggacaccg
     1381 aggacaccga ggacgccgag gcgttcgccg ccgaggccga cggggaggac accgaggaca
     1441 ccgaggacac cgaggacacc gaggacgccg aggacgccga ggccatgagc gccagcgacc
     1501 gcctgtcggc ccgcatcgcc gcggaccggg accgcgagcg ccgtgcgagg tggcaggccc
     1561 agctggcgcg agatcgccag cgggaagcgc aggccgccgg cgccgtccag cagagcctga
     1621 ccagccgcct gcaaggcatc cgcggctcgg gccgcaccgg caccgtgacg ctgggcaaca
     1681 tcaacggcgt gcgccgcatc gagatgaccc tggccaacgg gcgcaaggcc gtggtgcagg
     1741 tcgtgccgcc gctcgcctcg gcgaacgagg tcaaccggct gcgcgcggtc gtgaacacca
     1801 acgagaagcg ccgcgccaac tccgacgccg ccaactcgcg cgcgatcagc cggctggcct
     1861 ccgcccaggt cgccttcgtc aagcgccaaa ccgcgcagca ggtcaagagc gaccgcgagc
     1921 tgggcaagcg catcgtcgag ggctacagcc gcctggacaa gcgcatcacc accgagatcg
     1981 ccaaccagaa ggcgaacgtc gggaaggggg acaagcggat cttccggcgc ctgaagcggg
     2041 tccgctacca gctgctgatg aacgacgtgc tgctggccag cgcgctgccg ttcttcgcgg
     2101 cgtacggcaa gcaaggcagc ccgttcgaca agcgcaacct ggccctgacc ggcgtgctgg
     2161 ccggctggct cctgggcgac gagctcatcg gcctggccac cgggatgaac aagtccagca
     2221 aggccatgaa cacgctgcag acgacgtggg cgaccgtcgc gccgatcggc aacggcctca
     2281 ccgacttcct gctgtaccgg aacacgcaga actcgcggtt cctcacgggc accacgacca
     2341 ttcccgttcc gaccgcggga agtcccactc ccaaccaggt gaccgtgacc gtgccgatcg
     2401 cggtgagctc ggcctctacc ttcaataccc tgaaaaatcc accggtggtg gccacggtcg
     2461 tgagcggcca cgttcaaacg ggatcgcccc tgggcccgat cacacccacc atccaggcct
     2521 cgatcgatac gggtaagctg actctggtcg tgggcgaggg gaccatcacc gaagcgatcg
     2581 tggttgcctg ggtgatcgac accgaccccg gtgtcaaccc gacccacgtc gccgtctagc
     2641 cgaagctcgt cctccgacgg ttcgagggac ccgcggatgc cgatcaggcc caagctgcgc
     2701 cgcacggccc gcgaggcggt ggccgaggac tacctcgacg tcgcctcgcg gcgtccgggt
     2761 ggggaccccg tgggacgggc accgccggtg cgcggcaccc cgctggtgac cgggttcctc
     2821 gcgaacgggc gcgtggtccc gctgagcgtt cgcgagcccc tggcgccgct gggcgatctc
     2881 gaccagctgg tcgggaccct gcgccgcatg ggagacgacg ccgacgccgc gatccgggcg
     2941 cgttcccagg ccatcgtgcg gctgtcgcgc accgtcgggt ccgacgcggc gcaactgggc
     3001 cgcgagagcg gggcggcccg ccggaagctg gagcggcagg cgctggcggc gagcgccagg
     3061 ctggatcggc ggatcgacaa ggagctgatc ccgctgcgcg aggacatcgc gaaggccggc
     3121 gcgcgccacg agcggctggt gcggcggctc gctcgccgcg cgctgtggga cgacctggtg
     3181 gtgctgtcgg cgctgccgct gttcgcagcg ttcggccagc gcgacaaccc acttcactcc
     3241 agcaacctgg ccctgacgct gtcgctgctg gtgtggctgc tgggcgatga tctgaccgac
     3301 ctgatctccg gcgcggagac ggtgttcggt atccgggaca ccgacatctg gtcgtacatc
     3361 gctccgctcg ccaacgtgct caccggctgg ttcctgctgc gcaacgccca gaacgagcgc
     3421 ttcatcaccg ggttcgccag cgacttccgc ctggtcttcg tgaccgccga cgcgctgctg
     3481 agcgcaaaag ccggcgtggg gaagccggcc ggcgggacgg ccgacaccgg gctgtcggag
     3541 cgcttgttca agtttcgcca gcagatcgat ctgtcgctgt tcgtcgcgcc ggaccacttc
     3601 gacgccttcc tgggcttcga gcacatcccc gccgtggcca gcatcgcgca gatcacgtgg
     3661 ggccccgggc tggacgatcc ggccgcccgc cggatcgtcg ggctcacggc cgtcgcggag
     3721 gacggagtct tgaccatcac catcaccatc accgtcgccg tctcctacct caagcagctg
     3781 ccgcgccccg aggcggtcct gtcggcgctc cgcgcggcgt ggatcgtgga caccgcgcag
     3841 ccgaaggcat gaccatgccc acatccg
//
LOCUS       DASVOR010000376         3101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_324925,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000376 DASVOR010000000
VERSION     DASVOR010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3101
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324925"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            557..1027
                     /locus_tag="VF516_07675"
     CDS             557..1027
                     /locus_tag="VF516_07675"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HEX8107593.1"
                     /translation="MDQTPIVSLAAGHPLIDIDGTVIVQFVLFLVVFAVANRFLFQPY
                     LRLRERRKAGIEGARAEADRMTAQADAKLADYEKQLAVAREGANDEARKIRGEATRHE
                     REVTDQARAKAQQAIDDAQATVRRETEAARGQLLPQADALARSIASKLLGREVA"
     gene            1028..1954
                     /locus_tag="VF516_07680"
     CDS             1028..1954
                     /locus_tag="VF516_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107594.1"
                     /translation="MLAMHRMSWMSAMSAMNAMRGSIVAALAAGGLALAAAPAHAQPA
                     ENAGGSGAASEAATAAKQATAPFETTEGKNEGEKKLHAAGEEEADPSRHFNYVGIQPG
                     HLFDYSGKDELGGPLGDGKMTDPATGQTVHEEEPASPPFVFVLINFAILLGILAKWGA
                     PLARKTAQERHDLIKTALDEAARLRQQAADKLADYEARLLAADAEIKLLVEGMRADAD
                     KEKQRILAAAEAQAAQMARDAEQRIAAEIELARAQLTREVTAAAAAATEKLLREKLTA
                     GDQQQLVAGFIAGVQDSGQGGGRAPRGRGEVQ"
     gene            1954..2493
                     /gene="atpH"
                     /locus_tag="VF516_07685"
     CDS             1954..2493
                     /gene="atpH"
                     /locus_tag="VF516_07685"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HEX8107595.1"
                     /translation="MVTGSLARRYARAVIEIGTVHGNLDKIGADLRSLAHAMHASSEL
                     VTVLTNPAIRRADRRKVIDGLLQRIDAAPHTRNLVYLLLDGERMGSLEAISREVDAMI
                     EARAGRLAAEVTSARPLDAAQLSQITAALEQLSGKKVAVTRREDPSLLGGVVAKLGDT
                     VYDGSLRTQLRTLRDELTK"
     gene            2502..>3101
                     /locus_tag="VF516_07690"
     CDS             2502..>3101
                     /locus_tag="VF516_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAS-barrel domain-containing protein"
                     /protein_id="HEX8107596.1"
                     /translation="MDIRAAEISDIIRQQIENYDKKVSVTETGTVLTAGDGIARVYGL
                     SGAMAGELLEFEGAGGDKISGMVLNLEEDNVGVALLGKFEAVREGDVVKRTGRIVEVP
                     VGEELLGRVVNAIGMPVDGGKPIVGKQRRQVEIKAPGIIQRKSVHEPMQTGIKAIDSM
                     IPIGRGQRELIIGDRGVGKTAIAVDTIINQKGQNMHCFYV"
BASE COUNT          516 a         1041 c         1150 g          394 t
ORIGIN      
        1 gggtcggccg gccggtcggg ctggcgggtc cgcgtcgggt ccgcgggctg ggtctcggca
       61 aggcccccgt cgccgtcggg cttcatagtg gcccgcacga tcgtcgaagg gcccgcagcc
      121 cgatcgatgc ccctcgacat ccagcgcacg cgggccagca gagcaagcgc cgggccgagc
      181 ctcgaacagc gccaagtgtc cgtcgctgtt tcacccgaac cggaacgagc cgcccgaaat
      241 gttccggtgg cggttctggt ctgttccgat cgtccgcaag ctgcgcgaga ggaggaggtt
      301 cctccggagc agcggtcggt gttccggatg ttccggccgc tggtaagctg tcgatgttcc
      361 gggcgtagcg cggaacacgc ggtcgggacg gcgagatcct ggcagggcag cggttcgacc
      421 ggggcgtttg cgcggcgcat tgtcgcagtt ccgtcgacaa agctcgggca tcccaccgtt
      481 gacacggtgg tcggccccga ctataagcag ccggcgccaa cccaaggatt cttcataggt
      541 tgcgcaggta aagaccatgg atcagacgcc catcgtttcg ctggctgcag ggcatccact
      601 gatcgacatc gacggcacgg tgatcgttca gttcgtgctg ttcctggtgg tgttcgcggt
      661 cgcgaaccgc tttctgttcc agccgtacct gcggctgcgc gagcggcgca aggcggggat
      721 cgagggggcg cgcgccgagg cggatcggat gacggcgcag gccgacgcga agctggccga
      781 ctacgagaag cagctcgcgg tcgcgcgcga gggcgccaac gacgaggcgc gcaagatccg
      841 cggggaggcc acccgccatg agcgcgaggt gaccgaccag gcgcgggcca aggcgcagca
      901 ggcgatcgac gacgcgcagg ccaccgtgcg gcgcgagacc gaggccgcgc gcggccagct
      961 gctgccacag gccgacgcgc tggcgcgctc gatcgcgtcg aagctgctcg gacgggaggt
     1021 tgcctagatg ctggcgatgc accggatgag ctggatgagc gcgatgagcg cgatgaatgc
     1081 gatgcgtgga tcgatcgttg cggcgctcgc ggccggcggg ctcgcgctgg cggcagcgcc
     1141 ggcgcacgcg cagcccgccg agaatgcggg cggctccggg gccgcgtccg aggccgcgac
     1201 cgcggcgaag caggcgacgg cgccgttcga gaccaccgag ggcaagaacg agggcgagaa
     1261 gaagctgcac gcggccggcg aggaagaggc cgacccgtcg aggcacttca actacgtcgg
     1321 catccagccc ggccacctgt tcgactacag cggcaaggac gagctcggcg ggccgctcgg
     1381 cgacggcaag atgacggacc cggcgaccgg ccagaccgtg cacgaggagg agccggcgtc
     1441 gccgccgttc gtgttcgtgc tgatcaactt cgcgatcctg ctcgggatcc ttgccaagtg
     1501 gggggcgccg ctggcccgca agacggccca ggagcgccac gatctgatca agacggcgct
     1561 cgatgaggcg gcgcggctgc gccagcaggc tgccgacaag ctggcggact acgaggcgcg
     1621 gcttttggcg gccgacgccg agatcaagct gctggtcgaa gggatgcggg ccgacgccga
     1681 caaggagaag cagcggatcc tcgccgcggc cgaggcccag gcggcgcaga tggcgcgcga
     1741 cgccgagcag cggatcgccg ccgagatcga gctggcgcgc gcgcagctga cccgcgaggt
     1801 caccgccgcc gccgccgccg cgacggagaa gctgctgcgc gagaagctga ccgccggcga
     1861 tcagcagcag ctggtcgccg ggttcatcgc gggcgtccag gattccggcc agggcggcgg
     1921 ccgggcgccg cgcggccgcg gggaggtgca gtgatggtga ccggcagcct ggcccgccgc
     1981 tacgcgcgcg ccgtgatcga gatcggcacg gtccacggca acctcgacaa gatcggcgcc
     2041 gacctgcggt cgctcgcgca cgcgatgcac gcgtcgtccg agctggtcac ggtgctgacc
     2101 aacccggcga tccgccgcgc cgaccgccgc aaggtgatcg acgggttgct ccagcggatc
     2161 gacgccgcgc cccacacccg caacctggtg tacctgctgc tcgacggcga gcggatgggc
     2221 tcgctcgagg cgatctcgcg cgaggtcgac gcgatgatcg aggccagggc cgggcggctt
     2281 gccgccgagg tcacgtcggc ccggccgctc gacgccgccc agctgtccca gatcaccgcc
     2341 gcgctggagc agctgtccgg aaagaaggtc gcggtcaccc ggcgcgagga ccccagcctg
     2401 ctcggcgggg tcgtcgccaa gctcggtgac acggtctacg acggctccct tcgcacccag
     2461 ctccgcacgc tccgcgacga gctcaccaag taggaaccac catggacatc cgcgctgcag
     2521 agatctccga catcatccgc cagcagatcg agaactacga caagaaggtc tcggtcaccg
     2581 agaccggcac ggtgctgacc gccggcgacg gcatcgcccg ggtctacggc ctgtcgggcg
     2641 cgatggccgg cgagctgctc gagttcgagg gcgccggcgg cgacaagatc agcggcatgg
     2701 tgctcaacct cgaggaggac aacgtcggcg tcgcgctgct cggcaagttc gaggcggtgc
     2761 gcgagggcga cgtcgtcaag cgcaccggcc gcatcgtcga ggtgccggtc ggcgaggagc
     2821 tgctcggccg ggtggtcaac gcgatcggca tgccggtcga cggcggcaag ccgatcgtcg
     2881 gcaagcagcg ccgccaggtc gagatcaagg cgccgggcat catccagcgc aagagcgtgc
     2941 acgagccgat gcagaccggg atcaaggcga tcgactcgat gatcccgatc ggccgcggcc
     3001 agcgcgagct gatcatcggc gaccgcggcg tcggcaagac cgcgatcgcg gtcgacacga
     3061 tcatcaacca gaagggccag aacatgcact gcttctacgt g
//
LOCUS       DASVOR010000377         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_178989,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000377 DASVOR010000000
VERSION     DASVOR010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178989"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1141)
                     /locus_tag="VF516_07695"
     CDS             complement(<1..1141)
                     /locus_tag="VF516_07695"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEX8107597.1"
                     /translation="MSWWSKIFGKEPPKPREAPRAVWVPADQNRHGVPVLDLFAVTGQ
                     LLSTTSDPKNAATAISWASKLVADLELTLTPAESLACELRYPAEHDLPDGWLYVPSQM
                     EEKWAIAYRGAAIYLIRSWTGDVKAAGRVRRDGDEIVVECVELSDGMLRMFGDPIETF
                     DWILRAHALGQQVPLPIASDTAPMLEAAPLSVFSPFGHVAKCAATSWSPPPPQRPLRS
                     TGDVVTAVREEDEARLRALVAGGASLGARSAVLGYTALHVAVVKGSVALTRLLLELGA
                     DPNVLADRDACVLITAVVHRAPIEVLELLAKHGAIATPNADGFGLLHAIAETDRPEYL
                     EWGLARGLDLEARTKHGHTPLQVAAGLGHVAALRALLAAGADRAAK"
     gene            complement(1184..2092)
                     /locus_tag="VF516_07700"
     CDS             complement(1184..2092)
                     /locus_tag="VF516_07700"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX8107598.1"
                     /translation="MHPPRTRSPAETLAWVEWTIGSGARVVGWRRLTGGITSSVHRLT
                     VERNGRRGSFVLRRWVPDREHQEYGMRAVASETAVLTALERSDLPAPRLIGSTTDAAH
                     AGPAVLMTRVPGHVQLMPRDRERWLQQMAQTLTRIHALDIDAKPFQSWLDRSRLSPLP
                     DASRPDLWREAIALVAEERRPTQTRFIHRDYQHFNLFWSRERLTGVIDWVEASVGPPD
                     VDVGHCRLNLTVLFSADVADRFRDIYEAEAGRKVDPWWDVHSLLSYGPAWKRFLPIQI
                     DGRAPLDVDGMTRRMEDVLERTLRRS"
     gene            complement(2129..>2410)
                     /locus_tag="VF516_07705"
     CDS             complement(2129..>2410)
                     /locus_tag="VF516_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107599.1"
                     /translation="GHVMIMRSVPVPDGSLPSGPQGSTGYTVEVIDSTSTPHGATDWR
                     ALSAGQGLGHGTFVLYADATGTIVASRWSPSDSTDYDTTIHPLAIGGLQ"
BASE COUNT          362 a          877 c          835 g          336 t
ORIGIN      
        1 ccttcgccgc gcgatcggcg ccggccgcga gcagcgcgcg cagcgccgca acgtgcccca
       61 gccccgcggc aacctggagc ggcgtgtggc cgtgcttggt gcgcgcttcg aggtccagtc
      121 cgcgcgccag cccccactcc aggtactcgg ggcggtcggt ctccgcgatc gcatgcaaca
      181 gcccgaagcc atcggcgttc ggcgtcgcga tcgcgccgtg ctttgccagc agctcgagca
      241 cctcgatcgg cgcccggtgg accaccgcgg tgatcagcac gcacgcgtcg cgatccgcga
      301 gcacgttggg atcggcgccc agctcgagca acagccgggt gagcgcgacg ctgcccttga
      361 ccaccgcgac gtgaagagcc gtgtacccga ggactgcgct ccgcgcaccg agcgaggcgc
      421 cacctgccac cagcgcacgg aggcgcgcct catcctcttc gcggacggcg gtcacgacat
      481 ccccggtcga ccgcagcggc cgctgcggcg gcggcggcga ccagctcgtc gcagcgcact
      541 tcgcgacatg gccgaacggg ctgaacacgg acagcggcgc tgcctcgagc atcggagcag
      601 tatcgctggc gatcggaagc ggcacctgct ggcccagcgc atgcgcgcgc aggatccagt
      661 cgaacgtctc gatcgggtcg ccgaacatcc gcagcatgcc gtccgatagc tcgacgcact
      721 cgacgacgat ctcgtcgcca tcgcgccgca cccggccggc cgccttcacg tcgccggtcc
      781 agctacggat caggtagatc gccgcgccgc gatatgcgat cgcccacttc tcctccatct
      841 gcgagggcac gtacagccag ccatccggca gatcgtgctc cgcgggatac cgcagctcgc
      901 acgccagcga ttccgcaggg gtcaacgtca gctccagatc cgcgacgagc ttcgatgccc
      961 acgagatcgc cgtggccgca ttcttgggat cgctggtcgt cgagagcagc tgcccggtga
     1021 cggcgaacag atcgagcacg ggtacgccgt ggcggttctg gtcggccggt acccacaccg
     1081 cgcgcggcgc ctcgcgcggc ttcggcggct ccttcccgaa gatcttcgac caccacgaca
     1141 tggccgcaag ctaacacgcc tccatgggtc gcccgcccga cgctcacgac cggcgcagcg
     1201 tccgctcgag cacgtcctcc atccggcgcg tcatcccgtc gacgtcgagc ggcgcgcggc
     1261 cgtcgatctg gatcgggagg aatcgcttcc aggcgggtcc gtaggacagc agcgagtgga
     1321 cgtcccacca cgggtcgacc ttgcgcccgg cctcggcttc gtagatgtcc cggaagcggt
     1381 cggcgacgtc ggccgagaac aggacggtga ggttgaggcg gcaatggccg acgtcgacgt
     1441 ccggcggtcc gacggacgcc tcgacccagt cgatcactcc ggtcagccgc tcgcgcgacc
     1501 agaacaggtt gaaatgctgg tagtcgcgat gaatgaagcg cgtctgcgtc ggccggcgct
     1561 cctcggcgac cagcgcgatc gcctcccgcc agaggtcggg gcgcgacgcg tccgggagcg
     1621 gcgacagccg gctgcgatcg agccacgatt ggaatggttt ggcgtcgatg tcgagggcgt
     1681 ggatccgcgt gagcgtctgc gccatctgct gcagccagcg ctctcgatcg cgaggcatca
     1741 gctgcacgtg ccccggcacc cgcgtcatga ggacggccgg accggcgtgg gcagcgtcgg
     1801 tggtcgagcc gatcaggcgc ggcgccggga gatcgctgcg ctcgagcgcg gtgaggaccg
     1861 ccgtctccga tgccacggcg cgcatgccgt actcctggtg ctcgcggtcg ggcacccacc
     1921 ggcgcaggac gaacgacccg cgtcggccgt tccgctccac cgtgagccga tggacggacg
     1981 acgtgatccc gccggtgagc cgccgccatc cgaccacccg ggcgccggac ccgatggtcc
     2041 actcgaccca cgccagcgtc tcggccggcg atcgcgtccg gggcggatgc acccggccat
     2101 tgtcatccga gcgcgcgccg gtgtcacgct actgcaggcc gccgatcgca agcgggtgta
     2161 tcgtcgtgtc gtagtcagtc gaatccgacg ggctccagcg cgacgcgacg atggtcccgg
     2221 tggcatcggc gtacagcacg aacgtcccgt ggccgagccc ctgcccggcg ctcagtgcgc
     2281 gccagtcggt cgcgccgtgc ggtgtcgagg tcgagtcgat gacctcgacc gtgtagccgg
     2341 tcgagccctg ggggcccgac gggagcgagc cgtcggggac cgggacggag cgcatgatca
     2401 tgacgtggcc
//
LOCUS       DASVOR010000378         1618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_32716,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000378 DASVOR010000000
VERSION     DASVOR010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1618
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32716"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VF516_07710"
     CDS             complement(<1..967)
                     /locus_tag="VF516_07710"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8107600.1"
                     /translation="MAAISRIPRARARAIADRLGLLGRFSVLSLVSLAVLGLVLAHFF
                     SSRIHDRAVATAAQEAELMVNFGITPQLAGADLNQKMSPEAINTLDSLLQAGYTSHPV
                     KEIRIYNASRHVVYSDNRRVMGTVERAGGGLDSALRGRTWADESAGNDVDHAIEVYVP
                     LRYGDAGTPAVGAFEVYLDYAPVEAGIRADTNRVLLLLALGLMLVWGVLYRIVAGASG
                     RLRRQAAENEHQARHDGLTGLPNRGSFYDRVTAALESSRATGSLVSVMIVDLDRFKEV
                     NDTLGHHSGDELLREAGERLTTALRSGDVLSRLGGDEFAVLLPQVD"
     gene            complement(1031..>1618)
                     /locus_tag="VF516_07715"
     CDS             complement(1031..>1618)
                     /locus_tag="VF516_07715"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEX8107601.1"
                     /translation="RVIVSTYQSTSGTGRRAVEELHGQAQAVLGERPVERSVYPHQIA
                     FNVIPQVETFKDGDDYTTEERKMMAETRKILGDQDIRISATCARVPVYTGHSESVNVE
                     TRDPLEPDECRELLAKAPGVIVVDDPANGVYPQAIDAAGRDEVLVGRIRRDPSHERCL
                     NMWVVGDNLRKGAATNAVQLAELLHERDLLGTAAR"
BASE COUNT          226 a          558 c          584 g          250 t
ORIGIN      
        1 agtcgacctg cgggaggagc acggcgaact cgtcgccgcc gaggcgcgaa agcacgtcgc
       61 ccgaacgcag cgccgtggtc aggcgctcgc cggcctcgcg caggagctcg tcgccagagt
      121 ggtggccgag cgtgtcgttg acctccttga agcggtcgag gtccacgatc atcaccgaca
      181 cgagcgagcc ggtggccctt gagctctcga gagccgcggt cacgcggtcg tagaacgacc
      241 cgcggttcgg cagcccggtg agaccgtcgt ggcgagcctg gtgctcgttc tcggcggcct
      301 ggcgccgcag ccggcccgac gcgcccgcca cgatccggta caggacgccc catacgagca
      361 tgagcccgag ggcgagcagg aggaggacgc ggttggtgtc ggcgcggatg cccgcctcga
      421 ccggcgcgta gtcgaggtag acctcgaacg ctccgacggc cggggtgccg gcgtcgccgt
      481 agcgcagcgg cacgtagacc tcgatcgcgt gatcgacgtc gttgccggcg ctctcgtcgg
      541 cccatgtccg gccgcgcagc gccgagtcga gcccaccgcc ggcccgctcg acggtaccca
      601 tcaccctgcg gttgtccgag tacacgacgt gacggctcgc gttgtagatc cggatctcct
      661 tgacggggtg gctcgtgtag ccggcctgga gcagggagtc gagcgtgttg atcgcctccg
      721 ggctcatctt ctggttgagg tcggcgccgg ccagctgagg cgtgatgccg aagttcacca
      781 tcagctccgc ctcctgcgcg gccgtcgcca cggcgcggtc gtggatgcgg ctggagaaga
      841 agtgcgcgag gaccaggccg agcaccgcga gggacaccag gctgagcacg ctgaagcggc
      901 cgagcagccc gagccggtcg gcaatagcgc gtgcgcgggc gcgcgggatg cgcgaaatcg
      961 ccgccatctc tcgttgtgat cgtcctcagg agggtgaacc ttgaccccgg cctgggggta
     1021 ccgccgctcg ctagcgggcg gcggtgccga gcagatcgcg ctcgtggagc agctcggcga
     1081 gctgcaccgc gttggtggcg gcccccttgc ggaggttgtc gcccaccacc cacatgttca
     1141 ggcagcgctc gtgcgacggg tcgcggcgga tgcgcccgac gagcacctcg tcgcggcccg
     1201 cggcgtcgat cgcctgcggg tagacgccgt ttgccgggtc gtccaccacg atcacgccgg
     1261 gcgccttcgc cagcagctcg cggcattcgt ccggctcgag cgggtcacgc gtctcgacgt
     1321 tcaccgactc ggagtggccc gtgtacacgg gcacgcgcgc gcacgtggcc gagatccgga
     1381 tgtcctgatc gccgaggatc ttgcgcgtct cggccatcat cttgcgctct tcggtcgtgt
     1441 agtcgtcgcc gtccttgaac gtctccacct gcgggatcac gttgaacgcg atctggtgcg
     1501 ggtacaccga gcgctccacc gggcgttcgc cgagcaccgc ctgcgcctgg ccgtgaagct
     1561 cctcgaccgc gcgccggccc gtgcccgagg tggactgata ggtggagacg atcacgcg
//
LOCUS       DASVOR010000379         1681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_3626,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000379 DASVOR010000000
VERSION     DASVOR010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3626"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..842)
                     /locus_tag="VF516_07720"
     CDS             complement(<1..842)
                     /locus_tag="VF516_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HEX8107602.1"
                     /translation="MTPNELNDYVKKHGAKMIDAKFVDFLGQWKHLTYPIERLSEGLE
                     EGFGFDGSSISGWKAINSSDMLMKPDSTTAVIDPFLKTPTLSILCDVIDPITHESYSR
                     DPRSLAKRAVAYLKSTGIADTAYFGPEAEFFVFDSIQYSEDPNHSFFRVDSDEGGWNN
                     KREEGGGNKGSKPATKGGYCVVGPIDQISDLRTQMVMTMQAVGIVIETHHHEVASAGQ
                     CEIDMKYDTLVKMADQILWYKHIVKNVARAHGKTATFMPKPLFGDNGSGMHTHQSLWK
                     GGKPL"
     gene            complement(888..1226)
                     /locus_tag="VF516_07725"
     CDS             complement(888..1226)
                     /locus_tag="VF516_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEX8107603.1"
                     /translation="MKKVEAIIKPFKLDEVKERLAEIGVHGMTVTEVKGFGRTGGKKE
                     VYRGSAYVVDFVPKVKVEIIVPNDSVQQVVTVITEAARTGKIGDGKIFITSIDEVIRI
                     RTGETGEDAI"
     gene            complement(1313..>1681)
                     /locus_tag="VF516_07730"
     CDS             complement(1313..>1681)
                     /locus_tag="VF516_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107604.1"
                     /translation="SGGSGAGDEIEMNPDDPRDAKSGADPGAGRHAPARDIAHRPAHR
                     DVRPGQVAPPTPPPTREQLGQKFQQVRREYDAYKAKFGSRLEKEWGDLAMFIQYASED
                     DAWRRDAAHRLEELRAHMRE"
BASE COUNT          244 a          547 c          542 g          348 t
ORIGIN      
        1 agcggcttgc cgcccttcca cagcgactgg tgcgtgtgca tgccgctgcc gttgtcgccg
       61 aacagcggct tgggcatgaa cgtcgcggtc ttgccgtgcg cgcgggcgac gttcttgacg
      121 atgtgcttgt accagaggat ctggtcggcc atcttgacca gcgtgtcgta cttcatgtcg
      181 atctcgcact gacccgccga cgccacctcg tggtggtggg tctcgatgac gatgccgacc
      241 gcctgcatgg tcatcaccat ctgggtgcgc agatcggaga tctggtcgat cgggccgacc
      301 acgcagtagc cgcccttggt cgccggcttg gagcccttgt tgccgccgcc ttcttcgcgc
      361 ttgttgttcc agccgccctc gtcggagtcg acccggaaga acgagtggtt cggatcctcg
      421 gagtactgga tcgagtcgaa cacgaagaac tcggcctcgg ggccgaagta cgcggtgtcg
      481 gcgatcccgg tcgacttgag gtacgcgacc gcgcgcttgg cgaggctgcg cggatcgcgc
      541 gagtacgact cgtgggtgat cggatcgatc acgtcgcaca ggatcgacag cgtcggcgtc
      601 ttgaggaacg gatcgatcac cgcggtggtc gagtccggct tcatcagcat gtcggacgag
      661 ttgatcgcct tccagcccga gatcgacgag ccatcgaacc cgaagccctc ctccaggccc
      721 tcgctgagcc gctcgatcgg ataggtcagg tgtttccact ggccgaggaa gtcgacgaac
      781 ttcgcgtcga tcatcttcgc cccgtgcttc ttgacgtagt cgttgagctc gttcggcgtc
      841 acggttgtct cctgttatca gcctggaaca gacccctgga accagaatca gatcgcatcc
      901 tcgccggtct cgccggtgcg gatgcggatg acctcgtcga tcgacgtgat gaagatcttg
      961 ccgtcgccga tcttgccggt gcgcgcggcc tcggtgatca cggtgaccac ctgctgcacg
     1021 ctgtcgttgg gtacgatgat ctcgaccttg accttgggca cgaagtcgac gacgtaggcc
     1081 gagccgcggt acacctcttt cttgccgccg gtccgaccga agcccttgac ctcggtgacc
     1141 gtcatgccat gcaccccgat ctcggcgagg cgctccttga cctcgtcgag cttgaacggc
     1201 ttgatgatgg cctcgacctt cttcatctcg ccatggttag cgcagcgaat gtttcctgtg
     1261 ggtttcgctg cacgaaaatc gccggcacgc gagtggttcc gcgaagctgg actcactctc
     1321 gcatgtgggc ccggagctcc tcgagccggt gggcggcatc ccggcgccac gcatcatcct
     1381 cgctggcgta ctggatgaac atggcgagat ctccccactc cttttccagc cgggatccga
     1441 actttgcctt gtaggcgtcg tactcgcggc ggacctgctg gaacttctgg ccgagctgct
     1501 cgcgggtcgg cggcggggtc ggcggcgcga cctgccccgg ccgcacgtcg cggtgcgccg
     1561 ggcgatgcgc gatatcgcgc gccggtgcgt ggcggcccgc cccgggatcg gccccggact
     1621 tcgcgtcgcg ggggtcgtcc gggttcatct cgatttcatc tccggctccg gagccgccgg
     1681 a
//
LOCUS       DASVOR010000380         1889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_295857,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000380 DASVOR010000000
VERSION     DASVOR010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295857"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..393
                     /locus_tag="VF516_07735"
     CDS             151..393
                     /locus_tag="VF516_07735"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX8107605.1"
                     /translation="MKVKASVTLSEDLLRAVDELARSSSRSEVIERALRDFFAARARA
                     ERDARDLESLNAHADAYNVETADLTTYQTWIDDLAE"
     gene            383..778
                     /locus_tag="VF516_07740"
     CDS             383..778
                     /locus_tag="VF516_07740"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX8107606.1"
                     /translation="MPSKPRRPLPSRAAESAGQPWGAQLRRGELYRVRHPSGDTRRSR
                     VVVVVSRPAHLASRFSTAICAPVYSQRGGLSTEVEVGPTEGLKHPSAILCDVLTSFPR
                     GQLTDYVGTLGPAKLAALRAALRIAVDVE"
     gene            complement(782..>1889)
                     /locus_tag="VF516_07745"
     CDS             complement(782..>1889)
                     /locus_tag="VF516_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8107607.1"
                     /translation="WAGSCWYYLRFLSPQRDDVAWDADEERYWMPVDLYVGGNEHAVL
                     HLLYARFWHKVLHDAGLVSTREPFQRLFHQGMIHKPSFRDARGKYRAADEVEARGAPP
                     GGPELDPAKVNFAQASAATWYLKGSDEVVETKLDKMSKSRYNVVNPDDMAGEYGADAL
                     RLYELFMGPLEDGVEWETSGVAGTRRFLDRVWRVLVDADTGALSGKVSEDAPTGNREL
                     ERALHAAIKKVTQAITDLRFNTAIADMMVFVNEATKAQAVPRDWFEMFVKILSPFAPH
                     LGEELWQRLGHTTSVAHAPWPAHDEAKLARDTMVIAVQVGGKLRGQIEVAPDAAEPDI
                     LAAAQADPKVQSFVAGKPIKKAIYVKGRLVNLVV"
BASE COUNT          259 a          688 c          657 g          285 t
ORIGIN      
        1 gctcggcggc gcggtcaagt ccgcgcgcga gacgaccacc gcggccgcgc agctcgcgac
       61 caccgcggtc aacggcgtcg ccgcgccggc ctgagccgag caccgcaccc tggttgacat
      121 tcatcctgtg cccggtagga tgccggtagg atgaaagtga aggcgtcggt cacactgtcg
      181 gaagacctgc tccgggcggt cgatgagctc gcccggtcgt cgagccggtc cgaggtgatc
      241 gagcgcgcgc tgcgcgactt cttcgccgcc cgggcgcggg ccgagcgcga cgcgcgcgat
      301 ctggagagcc tgaacgcgca cgctgacgcc tacaatgtcg agaccgcgga tctgacgacc
      361 taccagacct ggatcgacga ccttgccgag taagccgcgg cgtccgctgc cgtctcgcgc
      421 ggccgagagc gcagggcagc cctggggcgc gcagctccgg cgcggcgagc tgtaccgcgt
      481 ccggcatccc agcggcgaca ccaggcgcag ccgcgtcgtc gtggtggtca gccgcccggc
      541 gcacctggcg tcgcggttct cgaccgcgat ctgcgcgccg gtctactcgc agcgcggcgg
      601 tctgtcgacc gaggtcgagg tcggccccac cgaggggctc aagcacccgt cggcgatcct
      661 gtgcgacgtg ctcaccagct tcccgcgcgg ccagctcacc gactacgtcg gcacgctcgg
      721 gcccgccaag ctcgccgcgc tgcgcgccgc gctacggatc gcggtcgacg tggagtgagc
      781 gtcacaccac caggttgacc agccggccct tgacgtagat cgccttcttg atcggcttgc
      841 cggcgacgaa cgactggacc ttggggtcgg cctgcgccgc cgccaggatg tccggctcgg
      901 ccgcgtcggg cgcgacctcg atctgaccgc gcagcttgcc gccgacctgg accgcgatga
      961 ccatcgtgtc gcgcgcgagc ttggcctcgt cgtgggccgg ccacggcgcg tgagcgaccg
     1021 aggtggtgtg gccgagccgc tgccacagct cctcgccgag gtgcggcgcg aacggcgaca
     1081 ggatcttgac gaacatctcg aaccagtcgc gcggcaccgc ctgcgccttg gtcgcctcgt
     1141 tgacgaacac catcatgtcg gcgatcgcgg tgttgaaccg caggtcggtg atcgcctggg
     1201 tgaccttctt gatcgccgcg tgcagcgcgc gctcgagctc gcggttgccg gtgggcgcgt
     1261 cctcgctgac cttgccggac agcgcgccgg tgtcggcgtc gaccagcacc cgccagaccc
     1321 ggtcgaggaa ccgccgcgtg cccgcgaccc cggaggtctc ccactcgacg ccgtcctcca
     1381 gcgggcccat gaacagctcg tagagccgca gcgcatcggc gccgtactcg ccggccatgt
     1441 cgtcggggtt gaccacgttg tatcggctct tggacatctt gtcgagcttg gtctcgacga
     1501 cctcgtcgct gcccttgagg taccaggtcg ccgcgctggc ctgcgcgaag ttgaccttcg
     1561 ccggatcgag ctccgggccg cccggtgggg cgccgcgggc ctcgacctcg tcggccgcgc
     1621 ggtacttgcc gcgcgcgtcg cggaacgacg gcttgtggat catgccctgg tggaacaggc
     1681 gctggaacgg ctccctggtc gagaccagcc cggcgtcgtg cagcaccttg tgccagaacc
     1741 gcgcatacag caggtggagc accgcgtgct cgttgccgcc gacgtagagg tcgaccggca
     1801 tccagtagcg ctcctcgtcg gcgtcccacg cgacgtcgtc gcgctgcggc gacaggaacc
     1861 gcaggtagta ccagcacgag cccgcccac
//
LOCUS       DASVOR010000381         1810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_559002,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000381 DASVOR010000000
VERSION     DASVOR010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1810
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559002"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..883
                     /locus_tag="VF516_07750"
     CDS             323..883
                     /locus_tag="VF516_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEX8107608.1"
                     /translation="MAKQDLHGLRVAILATDGFEQVELQEPRKALDEAGAKTMVVSPK
                     NDRIRGWKFTDWGDFVPVDVSLDQAKPNDFDALLQPGGVINPDKLRMIPRAVDFVKAF
                     FQAGKPVAAICHGPWMIIEAEAARGRRIASWPSLKTDLRNAGAEWVDQEVVVDGKLVS
                     SRKPDDIPAFNREMLSLFGKAVGKAA"
     gene            1197..1808
                     /gene="rpsD"
                     /locus_tag="VF516_07755"
     CDS             1197..1808
                     /gene="rpsD"
                     /locus_tag="VF516_07755"
                     /inference="COORDINATES: protein motif:HMM:NF003717.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX8107609.1"
                     /translation="MARYTGPRRRVLRRLGTELSGLTRKTAEARPHPPGQHGRKPRRR
                     RESDYAVRLREKQKVRYHYGVTEKQLRNYMRRAARSGEPTVELLRLLERRLDNVVFRI
                     GLAPTIPAARQLVRHRHILVNGRRVSAPGYQVDVGDEVVARAPSRAHPRIVEGASFGP
                     ELIVPRWLERARDGFGGRMIGQPERSEIPLDVNERLIVEFYAR"
BASE COUNT          298 a          599 c          655 g          258 t
ORIGIN      
        1 ctcgcgcggg cgggggggcg gcggaccggc cggcggtcac ggccgcggca ccgacccgac
       61 ggcgagctgc tcgcgctggc ggagtcgccg gcgccgacct gatggctgtc acgcacgcgg
      121 tggcgacggc ccggccagcc ggcggaacgg gtggttccag tcgccaacaa cgccgcgatc
      181 acatcattca ggccagcccg gcagcgctcc cgggtccggt tcgtgcggcg agtgagctgc
      241 ggggagccgt cgatcttctg aagcgccgtc ggcgtgggat ctgcagggtg atcgacatga
      301 aaccactaag gagtgatgtg tcatggcgaa gcaagatctt cacggactca gggtcgccat
      361 tctcgcgacc gacggcttcg agcaggtcga gctgcaggag cctcgcaagg cgctggacga
      421 ggccggcgcg aagacgatgg tcgtctcacc caagaacgat cggatccgcg gctggaagtt
      481 caccgactgg ggagacttcg ttcccgtcga cgtcagcctt gatcaggcaa aaccgaacga
      541 cttcgacgcg ctgctacagc ccggaggtgt gatcaatccc gacaagctgc ggatgattcc
      601 gcgcgccgtg gatttcgtga aggcattctt ccaggcaggc aagccggtgg ccgcgatctg
      661 tcacggaccg tggatgatca tcgaggcaga ggcggcacgc gggcggcgga tcgcgtcgtg
      721 gccgtcgctc aagacggacc tccgcaacgc gggtgccgag tgggtcgatc aggaggtggt
      781 cgtggacggc aagctggtgt cgagccgcaa gccggatgat atcccggcgt tcaaccgcga
      841 gatgctgtcg ctgttcggga aggcggtcgg gaaggcggcg tgacgccggc gccgtgggca
      901 gtgcggcggc atggacctcg gtgatcaccg cccgtaggac cccacgtacg gcgtcgaaga
      961 gcgccgcagg cacactgggc cgggcagcct cccatgagcg gacgtggacc tcgatgcagt
     1021 cggcagcgtc tcgacacccg tggttcgcga cccgctcatc gatcttgcga tgctccaggc
     1081 gatgcgcggc gccgcgatgc ggtgctctgg cgtcccatga gcgctcggtt ccggcacgtc
     1141 gcgtgcacca tgacaccatg catgattgac caccgtgacc atggcccgag gtgaacatgg
     1201 cgcgatacac gggaccacgg cggcgcgtgc tgcgccggct cggcacggag ctgagcgggc
     1261 tgacgcggaa gaccgccgag gcgcgccccc atccaccggg ccagcacggc cgcaagccga
     1321 gacggcgccg cgagagcgac tacgcggtcc ggctacgcga gaagcagaag gttcgctatc
     1381 actacggcgt caccgagaag cagctgcgca actacatgcg ccgcgcggcg cgcagcggcg
     1441 agccgaccgt ggagctgctg cgcttgctcg agcgccgtct cgataacgtg gtgttccgga
     1501 tcggcctggc gccgacgatc ccggccgcgc gacagctcgt gcgccaccgc cacatcctgg
     1561 tcaacggccg ccgggtctcg gcgcccggct accaggtcga cgtcggcgac gaggtcgtcg
     1621 cccgcgcgcc aagtcgtgcg cacccgcgga tcgtcgaggg cgcgagcttc ggcccggagc
     1681 tcatcgtacc ccggtggctc gagcgcgcac gcgacggctt cggcggccgg atgatcggcc
     1741 agccggagcg atccgagatc ccgctcgacg tcaacgagcg gctgatcgtc gagttctacg
     1801 cgcgctgatc
//
LOCUS       DASVOR010000382         4421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_354299,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000382 DASVOR010000000
VERSION     DASVOR010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354299"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1907
                     /locus_tag="VF516_07760"
     CDS             <1..1907
                     /locus_tag="VF516_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX8107610.1"
                     /translation="YDMVLSHGVEEYIDNKKKLVRYKFFRDEAHGGRDAVFGLDVPLM
                     RLMNVLKSAAQGYGTERRIILLHGPVGSAKSTIARQLKKGLEDYSRMPEGALYTYYWT
                     LPGALSDLAGGQESFPSPMHDEPLRLIPREWREQTIQRLRLGTDDHKVKIEGDVNPAC
                     RLIFKELMRHYDGYFDKVMNHIRVKRLVLSEQDRIGIGTFQPKDEKNQDSTELTGDIN
                     YRKIATFGSDSDPRAFNFDGEFNIANRGILEFVEILKLDVAFLYDLLGATQERKIKPK
                     KFAQTDIDEVILGHTNEAEYKKLLNNEFMEALRDRTIKVDIPYITKVSEEVKIYTKDF
                     TSQKVGKHIAPHTLHVAALWAVLTRLEDPKKHSLSLIQKAKLYDGKTLPGFTQDNIKE
                     LRKEANREGLDGISPRYIQDKIANSLVSDKADGAINPFMVMNELESGLRSHSLINSDE
                     QRKRYAEMLSVVKQEYEDVVKNEVQRAISADEDMIAKLCANYIDNIKAYTQKEKVKNP
                     YTGQDEEPDERLMRSIEEKIDIPESRKDDFRREIMNYIGALAIEGRTFDYKTNERLHK
                     ALEAKLFEDQKDSIKLTSLVSSVVDRDTQAKIDVVKQRLIKNFGYDEVSATDVLNFVA
                     SIFARGDVKS"
     gene            1919..2155
                     /locus_tag="VF516_07765"
     CDS             1919..2155
                     /locus_tag="VF516_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107611.1"
                     /translation="MGKGPVTIELNHTLAELLARLCVELSTSTSTDTGGGGDDPTGVL
                     SRALGLLEMAQRTRRHGGRLVFINERGEMADVVF"
     gene            2165..3274
                     /locus_tag="VF516_07770"
     CDS             2165..3274
                     /locus_tag="VF516_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF444 family protein"
                     /protein_id="HEX8107612.1"
                     /translation="MSQKIDLDHGRFRQIIRGKIKQNLRKYISQGEMIAKKGKDRVAI
                     PLPSVDIPHFKHGDKQQGGVGQGEGNVGDSLGGDGDQAPGKGEAGDRPGDHLLDIEVS
                     LQELAEIMGEELELPRIQPKGTEKIVAWKDKYSGIRTTGPESLRHFRRTYRQAIRRQI
                     AMGTYDAKNPIIVPIRDDKRYRSWRSEPLPQSNAVIIYMMDVSGSMGDEQKEIVRIES
                     FWIDTWLQSQYHGVISRYIIHDAMAKEVDRDTFFRTRESGGTMISSAYKLCAKMIADE
                     YPSELWNIYPFHFSDGDNWSVDDTHTCVDLLKKDVLPHVNLFCYGQVESPYGSGQFIK
                     DLTEHFDDDERVVTSEIKGKDAIMDSIKEFLGKGR"
     gene            3277..>4421
                     /locus_tag="VF516_07775"
     CDS             3277..>4421
                     /locus_tag="VF516_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX8107613.1"
                     /translation="MNAIKRFPAYLRDVQEDVESHAKNFGLDFFPIVYEVLDYKTMNE
                     VAAYGGFPTRYPHWRFGMDYEQLSKSYEWGMSKIYEMVINTNPAYAYLLEGNSLVDQK
                     LVMAHVCAHVDFFKHNYFFSKTNRKMIDGMANHAARVRRHMARHGQDTVEDFIDTCLS
                     LENLIDPMSAYIVRASKPKADDDEEADPRDKLGRLRAKGYMESFINPPEYLEAQRKKR
                     EEEAKRQKRRFPEQPQRDVMQFLIEHAPLDNWQTDVLEIVRDEAYYFAPQAMTKIMNE
                     GWASYWHSRILTEKTLTAAEVIDYADANSGVMATAPGRLNPYKLGVELFRNIEDRWNR
                     GQFGKEWDECDSMDQKRHWDRRTGLGRKRLFEVRKLYNDITFIDEFFT"
BASE COUNT          942 a         1424 c         1413 g          642 t
ORIGIN      
        1 tgtacgacat ggtgctctcg cacggcgtcg aggagtacat cgacaacaag aagaagctgg
       61 tccgctacaa gttcttccgc gacgaggcgc acggcgggcg cgacgcggtg ttcgggctcg
      121 acgtgccgct gatgcggctg atgaacgtgc tcaagagcgc cgcgcagggc tacggcaccg
      181 agcgccggat catcctcttg cacggcccgg tcggctcggc caagtcgacg atcgcgcgcc
      241 agctcaagaa gggcctcgag gactactcgc ggatgcccga gggcgcgctc tacacgtact
      301 actggacgct gcccggcgcg ctgtccgatc tcgccggcgg ccaggagagc ttcccgtcgc
      361 cgatgcacga cgagccgctc cggctgatcc cgcgcgagtg gcgcgagcag acgatccagc
      421 ggctccggct cggcaccgac gaccacaagg tcaagatcga gggcgatgtc aacccggcgt
      481 gccgcctgat cttcaaggag ctgatgcggc actacgacgg ctacttcgac aaggtgatga
      541 accacatccg ggtcaagcgc ctggtgctca gcgagcagga ccggatcggg atcggcacgt
      601 tccagcccaa ggatgagaag aaccaggact cgaccgagct gaccggcgac atcaactacc
      661 gcaagatcgc cacgttcggc tcggactccg acccgcgcgc gttcaacttc gacggcgagt
      721 tcaacatcgc caaccgcggc atcctcgagt tcgtcgagat cttgaagctc gacgtcgcgt
      781 tcctctacga cctgctcggt gcgacgcagg agcgcaagat caagcccaag aagttcgcgc
      841 agaccgacat cgacgaggtc atcctcggcc acaccaacga ggccgagtac aagaagctgc
      901 tcaacaacga gttcatggag gcgctgcgcg accgcacgat caaggtcgac atcccgtaca
      961 tcaccaaggt cagcgaggag gtgaagatct acaccaagga cttcacgtcc cagaaggtcg
     1021 gcaagcacat cgcgccgcac acgctgcacg tcgccgcgct gtgggcggtg ctgacccggc
     1081 tcgaggaccc caagaagcac tcgctgtcgc tgatccagaa ggccaagctg tacgacggca
     1141 agaccctgcc cgggttcacc caggacaaca tcaaggagct gcgcaaggag gccaaccgcg
     1201 agggcctcga cgggatctcg ccgcgctaca tccaggacaa gatcgccaac tcgctggtca
     1261 gcgacaaggc cgacggcgcg atcaacccgt tcatggtgat gaacgagctc gagagcgggc
     1321 tgcgcagcca ctcgctgatc aactccgacg agcagcgcaa gcgctacgcc gagatgctgt
     1381 cggtggtcaa gcaggagtac gaggacgtcg tcaagaacga ggtccagcgc gcgatcagcg
     1441 ccgacgagga catgatcgcc aagctgtgcg cgaactacat cgacaacatc aaggcctaca
     1501 cccagaagga gaaggtcaag aacccgtaca ccggccagga cgaggagccc gacgagcggc
     1561 tgatgcgctc gatcgaggag aagatcgaca tcccggagag ccgcaaggac gacttccggc
     1621 gcgagatcat gaactacatc ggcgcgctcg cgatcgaggg ccggacgttc gactacaaga
     1681 ccaacgagcg gctgcacaag gcgctcgagg ccaagctgtt cgaggaccag aaggactcga
     1741 tcaagctgac ctcgctggtg tcgagcgtgg tcgaccgtga cacccaggcc aagatcgacg
     1801 tcgtcaagca gcggctgatc aagaacttcg gctacgacga ggtgtcggcg accgacgtgc
     1861 tgaacttcgt ggcgtcgatc ttcgcgcgcg gagacgtcaa gtcgtaatga gcgacccgat
     1921 gggtaagggg ccggtgacga tcgagctcaa ccacacgctg gccgagttgc tcgcccggct
     1981 gtgcgtcgag ctgtccacgt ctacgtctac tgatactggt ggcggcggcg acgatccgac
     2041 cggcgtgttg tcgcgtgcgc tcggcctgct cgagatggcg cagcgcaccc ggcgccacgg
     2101 cggccggctc gtgttcatca acgagcgcgg cgagatggcc gacgtcgtgt tctaggaggc
     2161 ccgcatgtcg cagaagatcg atctcgatca cggtcggttc cgccagatca tccgcggcaa
     2221 gatcaagcag aacctccgca agtacatctc gcagggcgag atgatcgcca agaagggcaa
     2281 ggaccgggtc gcgatcccgc tgccgtcggt cgacatcccg cacttcaagc acggcgacaa
     2341 gcagcagggc ggggtcgggc agggcgaggg caacgtcggc gactcgctgg gcggcgacgg
     2401 cgaccaggcg ccgggcaagg gcgaggccgg cgaccgcccc ggcgatcacc tgctcgacat
     2461 cgaggtctcg ctgcaggagc tcgccgagat catgggcgag gagctcgagc tgccgcggat
     2521 ccagcccaag ggcaccgaga agatcgtcgc gtggaaggac aagtactccg ggatccgcac
     2581 caccggcccc gagtcgctgc ggcacttccg gcggacctat cggcaagcga tccggcgcca
     2641 gatcgcgatg gggacgtacg acgccaagaa cccgatcatc gtgccgatcc gcgacgacaa
     2701 gcgctaccgg tcgtggcgca gcgagccgct gccgcagtcc aacgcggtca tcatctacat
     2761 gatggacgtg tcgggctcga tgggcgacga gcagaaggag atcgtccgga tcgagagctt
     2821 ctggatcgat acgtggctgc agtcgcagta ccacggcgtg atcagccgct acatcatcca
     2881 cgacgcgatg gccaaggagg tcgatcgcga cacgttcttc cggacccgcg agtccggcgg
     2941 cacgatgatc agctcggcct acaagctgtg cgcgaagatg atcgccgacg agtacccctc
     3001 ggagctgtgg aacatctacc cgttccactt ctccgacggt gacaactggt cggtcgacga
     3061 cacccacacc tgtgtcgacc tgctcaagaa ggacgtgctg ccgcacgtca atctgttctg
     3121 ctacggccag gtcgagtcgc cctacggctc gggccagttc atcaaggacc tgaccgagca
     3181 cttcgatgac gacgagcggg tcgtgaccag cgagatcaag ggcaaggacg cgatcatgga
     3241 ctcgatcaaa gagttcctcg gaaagggccg ctagcgatga acgcgatcaa gcggttcccg
     3301 gcctacctgc gcgacgtcca ggaggacgtc gagtcccacg ccaagaactt cgggctcgac
     3361 ttcttcccga tcgtctacga ggtcctcgac tacaagacaa tgaacgaggt cgccgcgtac
     3421 ggcggcttcc cgacccgcta tccgcactgg cggttcggca tggactacga gcagctgtcc
     3481 aagagctacg agtggggcat gtcgaagatc tacgagatgg tgatcaacac caatcccgcc
     3541 tatgcctacc tgctcgaggg caactcgctg gtcgaccaga agctggtgat ggcgcacgtg
     3601 tgcgcccacg tcgacttctt caagcacaac tacttcttct cgaagaccaa ccgcaagatg
     3661 atcgacggga tggccaatca cgccgcgcgg gtgcgccgcc acatggcgcg ccacggccag
     3721 gacaccgtcg aggacttcat cgacacctgc ctgtcgctgg agaacctgat cgacccgatg
     3781 tcggcgtaca tcgtgcgcgc gtccaagccc aaggccgacg acgacgagga ggccgatccg
     3841 cgcgacaagc tcggacggct gcgcgccaag ggctacatgg agtcgttcat caacccgccg
     3901 gagtacctcg aggcccagcg caagaagcgc gaggaggagg ccaagcgcca gaagcggcgg
     3961 ttccccgagc agccgcagcg cgacgtcatg cagttcctga tcgagcacgc gccgctcgac
     4021 aactggcaga ccgacgtgct cgagatcgtg cgcgacgagg cgtactactt cgcgccccag
     4081 gcgatgacca agatcatgaa cgagggctgg gcctcgtact ggcactcgag gatcctcacc
     4141 gagaagacgc tgaccgccgc cgaggtcatc gactacgccg acgccaactc gggggtgatg
     4201 gcgaccgcgc cgggccggct caacccgtac aagctcggcg tcgagctgtt ccgcaacatc
     4261 gaggaccgct ggaaccgcgg ccagttcggc aaggagtggg acgagtgcga ctcgatggac
     4321 cagaagcggc actgggatcg ccgcaccggc ctggggcgca agcggctgtt cgaggtccgc
     4381 aagctctaca acgacatcac gttcatcgac gagttcttca c
//
LOCUS       DASVOR010000383         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_179038,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000383 DASVOR010000000
VERSION     DASVOR010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179038"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            627..1826
                     /locus_tag="VF516_07780"
     CDS             627..1826
                     /locus_tag="VF516_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107614.1"
                     /translation="MRAAFAAIVLTAALLGAARAEPSGGTDFGAEARVMFRVAACGGD
                     DAVPERFSARAIAAHCRDMAEIYGSYKKAWAEPAQAFIAGLRPKDLPRVVVYPFGGGD
                     LSSALSVYPDATELTTISLEAAGDVRVIDTIKSAKLDEEVDRIGNEIHRLYYAAHSTT
                     KSLQAASHAELPGTIMFALAGLAVHGMEPVALRYFDIEPDGSLSYLTNAQLDERAAEF
                     AAKKLGAAPPRKITHYWYEQVSAFANVEIQFRPRGDARAPLRTYRHIVANLDDSHMAA
                     DDRVLRHLRGKGKVAVMTKAASFLLWYDDFSKIRDYLLHHAAWMISDASGIPPSFAEP
                     AGFEEITYGEFTGPYFVIDDKNVRAEFVKLWKAQPHRDLPFRFGYPDAAKHNHLMIMR
                     PRADALR"
     gene            1879..>2059
                     /locus_tag="VF516_07785"
     CDS             1879..>2059
                     /locus_tag="VF516_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107615.1"
                     /translation="MLRRMDRLALAHGACVIVELDDEAGALARLAPAEQDRAAQLGPI
                     RRRDFIAGRTALHLAL"
BASE COUNT          329 a          684 c          764 g          282 t
ORIGIN      
        1 atcgacgccg acacgatcgc cggaccgtgg gacggcacgc cggtcgacta cggacggatc
       61 gtggggcacc tcgcggcggc cgaaggcctg gcatgactcc ctgcgaggct catcgacgcg
      121 tgggcaacat ccgggcagcg cagggcgaag cgcaccggcg gagaccacgc gaagagctcg
      181 ccccggtgcc gcagggcggc tcggccgcgg aggaggggcg atccgttgag ggcacaaatc
      241 tgtcgtggat atcgaagcta tcaggatgct gagcacacca tctgtcagct gaaggcaagt
      301 agtccaagca cgacggcgat gtcgagagag gtcgccggtg tggcgcagtc gcccacgccg
      361 caggtggccc atgccgcagg tggccggacc acgcccagat caggcgggcg cagatgtgat
      421 cgacgacgac aggacaaacg cggagccctc cacgacgtga accagatgac cagccagtga
      481 ccggctgtgt cagcggtgct cgcatgtcga ggcagggcgc ctcacgcgcg tctccggatg
      541 gcgcatcgtc tcgggcggct tgggtgcggc cgttccaccg aaccgacggc gatcgtgcta
      601 cgtcacctgc agccctggga gccctgatgc gagctgcgtt tgctgcgatc gtcctcaccg
      661 ccgcgctgct gggcgcggcg cgcgccgagc cgtccggcgg caccgacttc ggggcggagg
      721 cgcgggtgat gttccgggtc gcggcgtgcg gcggcgacga tgcggttccg gagcggttct
      781 cggcccgggc gatcgcggcg cactgccggg acatggcgga gatctacggg tcgtacaaga
      841 aggcgtgggc cgagccggcc caggcgttca tcgcggggct gcggcccaag gatctgccgc
      901 gggtcgtggt gtatccgttc ggcggcggcg acctgtcgtc ggcgctgtcg gtgtatcccg
      961 acgcgaccga gctgacgacg atctcgctcg aggccgcggg cgatgtgcgc gtgatcgaca
     1021 cgatcaagag cgccaagctc gacgaggagg tcgaccggat cggcaacgag atccaccggc
     1081 tgtactacgc ggcgcactcg acgacgaaga gcctgcaggc ggcgtcgcac gccgagctgc
     1141 ccgggacgat catgttcgcg ctcgccgggc tggcggtgca cgggatggag ccggtggcgc
     1201 tgcgctactt cgacatcgag cccgacgggt cgctgagcta cctgacgaac gcccagctcg
     1261 acgagcgcgc ggccgagttc gcggccaaga agctcggggc cgcgccgccc aggaagatca
     1321 cgcactactg gtacgagcag gtgtcggcgt tcgccaacgt cgagatccag ttccggccgc
     1381 gcggcgatgc ccgggcgccg ctgcgaacgt accgccacat cgtggccaac ctcgacgaca
     1441 gccacatggc ggccgacgac cgtgtgctcc gccacctgcg cggcaagggc aaggtggcgg
     1501 tgatgaccaa ggcagcgagc ttcctgctgt ggtacgacga cttctcgaag atccgggact
     1561 acctgctgca ccacgcggcg tggatgatct cggatgcgtc ggggatcccg ccgagcttcg
     1621 cggagccggc cggattcgag gagatcacgt acggcgagtt caccgggccg tacttcgtga
     1681 tcgacgacaa gaacgtccgc gccgagttcg tcaagctgtg gaaggcgcag ccgcaccgcg
     1741 atctgccgtt ccggttcggc taccccgacg cggccaagca caaccacctg atgatcatgc
     1801 ggccgagggc cgacgcgctc cggtaggccg gctcgcgggc gggtggatcg cgcgagccgc
     1861 cgcggggata cttgcggggt gctgcgacgc atggaccggc tcgcgctggc gcacggcgcg
     1921 tgcgtgatcg tcgagctcga cgacgaggcc ggggcgctgg ccaggctggc gcccgccgag
     1981 caggaccgcg cggcgcagct cggcccgatc cgccgccgcg acttcatcgc cggccgcacg
     2041 gcgctgcacc tggcgctgg
//
LOCUS       DASVOR010000384         3530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412092,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000384 DASVOR010000000
VERSION     DASVOR010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412092"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VF516_07790"
     CDS             <1..552
                     /locus_tag="VF516_07790"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEX8107616.1"
                     /translation="LTITAADSAEQRRRIAGRAALATWVTLSVFALAGGLIFRAFGIS
                     FSAFKVAGGLMLLLMSIDMMRAQPSPTRTTAEEQGEASTRDDIAIFPLAIPMLAGPGA
                     IATVTVLMSRAAWDPVRTAAVFGAVTVSCVAAWLLMRWATRAERFLPKTLLRALERIM
                     GLLLAAVAVEFIASGARELLHAP"
     gene            622..1158
                     /locus_tag="VF516_07795"
     CDS             622..1158
                     /locus_tag="VF516_07795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107617.1"
                     /translation="MILPIPYLALIAVALGVVVLWWWLSRTPVTAPSAPIPMIPQRIA
                     FPGGLRLRDPERSLTALDKPDEIVIPFAHAVLVIDYPLTTPASVPIEAPLPLGFTRAA
                     LAKTICDEYAHIYDAEEGTAATKTIPIEERGGTRGRNRTDGAYGIWGHDLQDLVVTAA
                     RWTRQSDGTVQIELHVES"
     gene            complement(1165..2229)
                     /locus_tag="VF516_07800"
     CDS             complement(1165..2229)
                     /locus_tag="VF516_07800"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8107618.1"
                     /translation="MTEQVFIVGTGRCGSTLLSSVLRLHPEVTSLSEFLSFATDLGSQ
                     IAATFPDGEVDGMWLWQLIASPWPRQNLMIRHDVAMDEVLYPWRDRRHRFDRDTGVPA
                     ISQVTLPHLTDDPDGWLDALEPVVKAFERAPVGVQLRRLFDWLAARDGGAIWVERSGG
                     SLRVVHRLIEHFPDARFVHLVRDGRDTALSMSRHRGFKMVLACFQMLEMLGCDPFQSD
                     DRRWEADLPDDLAALLPERFTRQAFCDFEVPPPLCGHYWSGEIVEGLAVLDRLPPERL
                     LTLRFEDLFDDPEAVLRRLLTYLLGTDHVDAGFLARAATLVHRPASRWQDLEARDRGW
                     LEATCQAGFAALAARGYTWR"
     gene            complement(2232..3362)
                     /locus_tag="VF516_07805"
     CDS             complement(2232..3362)
                     /locus_tag="VF516_07805"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8107619.1"
                     /translation="MIATHFVTGATGLVGSAIVLELLQRTTDDVFCLVRPGSEDVTAR
                     LRRVLVRAAESYAAPPEVKRAIAERCHAVAGDVIEPCKLPPAPDRRFTQFWHSAASLH
                     YEARYADEIMAVNCQGTARALELAAQLGVSQFNHLSTAYVAGARQGEIAEAACPRDTR
                     PSNLYEKSKIEAEALVERDPRFARCILRPSIVIGHSRTLETINPTGLYAFLRKLRAYR
                     GLLARTQASLVHEKSLRLRVDAGTELDLIPIDRVACNAVSIALAHDPPPIRYFHLTNP
                     FAPQADMATEVMFETSGLARPTFVPDSSAFDWLDEKFNTQIEFYRTYLTGIRTFLRHN
                     TVRAVGPEPAEPYRIPRDAMRRYCAWYVARLDRARVDLPPTR"
BASE COUNT          531 a         1210 c         1271 g          518 t
ORIGIN      
        1 ctcaccatca ccgcggcgga ctcggccgag cagcgccgcc ggatcgccgg gcgcgccgcg
       61 ctcgcgacct gggtgacgct gtcggtgttc gcgctcgccg gcgggctgat cttccgcgcg
      121 ttcggcatct cgttcagcgc gttcaaggtc gccggcgggc tgatgctgct cctgatgtcg
      181 atcgacatga tgcgcgccca gccctcgccg acccggacca ccgccgagga gcagggggag
      241 gccagcacgc gcgacgacat cgcgatcttc ccgctcgcga tcccgatgct cgccggcccc
      301 ggcgcgatcg ccaccgtgac ggtgctgatg tcgcgcgcgg cctgggaccc ggtgcgcacc
      361 gccgcggtgt tcggcgcggt caccgtcagc tgtgtcgccg cctggctcct catgcggtgg
      421 gcgacccgcg ccgagcggtt cctgcccaag acgctgctgc gcgcgctgga gcgcatcatg
      481 ggtcttctgc tcgcggccgt cgcggtcgag ttcatcgcga gcggcgcccg ggagctcctg
      541 cacgcgccgt gaccgtgtgc tcacgcgccc gccgagcgcc gggcggctgc gcgacgggcg
      601 cggcggtgat agcttggcgc ggtgatcctg ccgatcccct acctcgcgct gatcgcggtg
      661 gcgctcggcg tcgtggtgct gtggtggtgg ctgtcgcgca cgccggtgac cgcaccgtcc
      721 gcgccgatcc cgatgatccc gcaacgaatc gcattcccgg gcggtctccg cctgcgcgat
      781 cccgagcgct cgctcaccgc gctcgacaag ccggacgaga tcgtcatccc gttcgcccac
      841 gccgtgctgg tcatcgacta cccgctgacc acgccggcct cggtcccgat cgaggccccg
      901 ctgccgctcg gcttcacccg cgcggcgctg gccaagacga tctgcgacga gtacgcccac
      961 atctacgacg ccgaggaggg caccgcggcg accaagacga tcccgatcga ggagcgcggc
     1021 gggacgcgcg gccgcaaccg caccgacggg gcctacggga tctggggcca cgacctccag
     1081 gatctcgtgg tcaccgccgc gcgctggacc cgccagtccg acggcacggt gcagatcgag
     1141 ctccacgtcg agagctgagc cggatcatcg ccaggtgtac ccgcgcgcgg cgagcgccgc
     1201 gaagcccgcc tggcaggtcg cttcgagcca cccgcgatcc cgggcctcga ggtcctgcca
     1261 ccgcgatgcc ggccggtgga cgagcgtcgc ggcgcgcgcc aggaacccgg cgtccacgtg
     1321 gtcggtcccg aggaggtagg tcaggaggcg tcgcagcacg gcctccgggt cgtcgaacag
     1381 gtcctcgaag cggagcgtga gcagccgctc cggcgggagc cggtcgagca ccgccagccc
     1441 ctcgacgatc tcgccggacc agtagtggcc gcacagcggc ggtggcacct cgaagtcaca
     1501 gaacgcctgg cgcgtgaacc gctccgggag cagcgcggcg agatcgtccg gcaggtccgc
     1561 ttcccagcgg cggtcgtcgg actggaacgg atcacagccg agcatctcca gcatctggaa
     1621 gcaggcgagg accatcttga agccgcgatg ccggctcatc gacagcgcgg tgtcgcgacc
     1681 atcgcgcacg aggtggacga accgggcgtc cgggaagtgc tcgatcagcc ggtgcaccac
     1741 gcgcaggctg ccgcccgagc gttcgaccca gatcgcgccg ccgtcgcgcg ccgcgagcca
     1801 gtcgaacaac cgccgcagct ggacgcccac cggcgcacgc tcgaacgcct tcaccaccgg
     1861 ctcgagcgca tcgagccagc cgtcggggtc gtcggtgagg tgcgggagcg tcacctgcga
     1921 gatcgcgggg acgccggtgt cgcggtcgaa gcgatgcctc cgatcgcgcc acgggtagag
     1981 cacctcgtcc atcgcgacgt cgtgccggat catcaggttc tggcgcggcc agggcgaggc
     2041 gatgagctgc cacagccaca tgccatcgac ctcgccgtcg gggaacgtcg cggcgatctg
     2101 gctgccgagg tcggtcgcga acgacaggaa ctccgagagg ctggtcacct cgggatgcag
     2161 ccggaggacg ctggacagga gcgtcgaccc gcaccggccg gttccgacga tgaacacctg
     2221 ctccgtcatg gtcagcgggt gggcgggaga tccacccgtg cccggtcgag tcgcgcgacg
     2281 taccacgcgc agtatctccg catcgcgtcg cgtggaatcc ggtacggctc ggcgggctcc
     2341 ggcccgacgg cacgcaccgt gttgtggcga aggaaggtcc ggattccggt caggtaggtt
     2401 cgatagaact cgatctgggt attgaatttc tcgtccagcc agtcgaaggc ggaggagtcc
     2461 gggacgaacg tggggcgagc gaggcccgag gtctcgaaca tgacctcggt ggccatgtcg
     2521 gcctgcggcg cgaaggggtt cgtgagatga aagtagcgga tcggcggtgg atcatgggcc
     2581 agggcgatcg agacggcatt gcatgccacg cgatcgatcg ggatcaggtc gagctcggtg
     2641 ccggcatcga cccgcaggcg gagcgatttc tcgtgaacca ggctggcctg ggtgcgggcg
     2701 agcagtcccc ggtacgcgcg cagcttgcgc aggaacgcgt agagtccggt cgggttgatc
     2761 gtttccagcg tgcggctgtg accgatgacg atgctcggcc ggaggatgca gcgcgcgaag
     2821 cgcggatcgc gctcgacgag cgcctccgcc tcgatcttgc tcttctcgta caggttgctg
     2881 ggccgcgtgt cgcgcgggca cgcggcctcc gcgatctcgc cctgacgagc gccggcgacg
     2941 tacgcggtgc tcaggtggtt gaactgcgat acgccgagct gcgccgcgag ctcgagggcg
     3001 cgggcggtgc cctgacagtt gacagccatg atctcgtccg catatcgcgc ttcgtagtga
     3061 agcgatgcgg cgctgtgcca gaactgggtg aagcggcgat cgggagccgg cgggagcttg
     3121 cagggctcga tgacgtcgcc ggcgaccgcg tggcagcgct cggcgatcgc gcgcttgacc
     3181 tccggtggtg ccgcgtagga ctcggcggcg cgcaccagca cgcgccggag ccgcgcggtc
     3241 acatcctcgg agccgggacg gaccaggcag aacacgtcgt cggtcgtgcg ctgcaggagc
     3301 tcgaggacga tggcagagcc gaccaggcct gtcgcgccgg ttacgaaatg ggtggcgatc
     3361 atgcagtctc ccgggataca ccggattcca ccacccggag acgtcggcgt gttcagctgc
     3421 aggtgtgttt cacgcaccgt tcgtgaacga gccgatgttt acgcgggata catttgtcca
     3481 cgcgtgcggt aaacctggga gatcgagcag ctccacgacc acaggtgacg
//
LOCUS       DASVOR010000385         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_354308,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000385 DASVOR010000000
VERSION     DASVOR010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354308"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..1673)
                     /locus_tag="VF516_07810"
     CDS             complement(120..1673)
                     /locus_tag="VF516_07810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107620.1"
                     /translation="MMGCDRGEEESPPVAAPPLTPQQEHGRDVWLHSTFGGEKFFSLI
                     LPEAPFNLRLGFDALLATPRSQRFTQWGAINDPDCTDGDGTTGFDRCTDPHATGIVGV
                     RKFANPLPVGPRVLFGVACASCHAGLDPARPPDDPNHPSWANIHLTTGNQYFDIAKIF
                     NAHLSPSDPRWQVFHTWARGTVDTTVLETDHINNPSIITQIFNLPDRPYFHLHDDGMP
                     TTVHRMGFGGEDDVGCQKAALRVYFNIGMCAAECMVGHLANGPNGSQTPIDLAQCRRD
                     CPEFRDAERNIPDLCAFLDTARPPKLPARYIDHAVVDRGKQVFLRACSHCHSNGRPIG
                     RRNVLSNDLLHRAPGFGLEPPGAVGTNSCRARTTNWQAGHIWATFSSDEKKALGPGYY
                     RNVPLLAVWATAPFLHNNRLGDFNGDPSIAGRLAAYDDAMDQLLNPGRRDFLGSIQRT
                     TQAIVLPTPLGGVPLPAGTPVAAFANLDPANPLDNLCPDLVENEGHWFGASLSASDKH
                     ALKEFLKTQ"
     gene            complement(2108..>2819)
                     /locus_tag="VF516_07815"
     CDS             complement(2108..>2819)
                     /locus_tag="VF516_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8107621.1"
                     /translation="ARNGFRLNGRPHELFVLRHLTSELHRQEVRTWKKVIRVISHELN
                     NSLAPIASLAHSGGELLKRGKHDQLDKVFATIAERARHLEQFILGYARFAKLPAPRPE
                     PTAWADLVERLRGQVAFQVDGALPDTRGHFDPAQLEQALVNLLRNAHESGSPPAEVQL
                     RVRKLPGAVAIEVSDRGTGMTEAVLSHALLPFYSTKRGGTGLGLALVREIAEAHGGRV
                     ALANRPGGGVSVTLTLPQ"
BASE COUNT          456 a          916 c         1000 g          447 t
ORIGIN      
        1 gccaccgtcg tccacatcgc ccgcgacccc gcctcggacg cgccccgcgt gatcggcatc
       61 tggcgcgagt agaccgtcgg gtacctggga tcgtgcgacg agctcacgac ctcggccgac
      121 tactgcgtct tcaagaactc cttgagcgca tgcttgtccg acgccgacag cgatgccccg
      181 aaccagtgtc cctcgttctc gacgagatcc ggacacaggt tgtccagagg attggcgggg
      241 tcgaggttcg cgaacgcggc gaccggcgtg cccgcaggca gcggaacacc gccgagcggc
      301 gtcgggagca cgatcgcctg cgtcgtgcgc tggatcgagc ccaggaagtc gcgccgcccc
      361 gggttgagca gctggtccat ggcgtcgtcg tacgcggcga ggcgccctgc gatcgacgga
      421 tcgccgttga agtcaccgag ccggttgttg tgaaggaacg gcgccgtcgc ccacaccgcg
      481 agcaacggga cgttgcggta gtagccggga ccgagcgcct tcttctcgtc cgatgagaac
      541 gtcgcccaga tgtgacccgc ttgccagttg gtggtgcgag cgcgacagct gttcgtaccg
      601 accgccccag ggggttcgag cccgaagccg ggcgcgcgat gcagcagatc attggatagc
      661 acgttacggc ggccgatcgg tcggccgttc gagtggcagt ggctacaggc gcggaggaat
      721 acctgcttgc cgcgatcgac caccgcgtga tcgatgtagc gcgcgggcag cttcggcggc
      781 ctggccgtgt ccaggaacgc gcacaggtcg gggatgttac gctcggcgtc ccggaactcc
      841 gggcagtccc ggcggcactg ggcgagatcg atcggggtct gcgagccatt cgggccattc
      901 gcgaggtgac caaccatgca ctcggcggcg cacatgccga tgttgaagta cacgcgcagg
      961 gcagccttct gacagccgac gtcatcctct ccgccaaacc ccatgcgatg cacggtggtc
     1021 ggcatgccgt cgtcgtggag atggaaatat gggcgatcgg ggaggttgaa gatctgcgtg
     1081 atgatgctcg ggttgttgat gtgatcggtc tcgagcaccg tcgtgtcgac ggtgccgcgc
     1141 gcccaggtgt ggaacacctg ccacctcgga tcgctcggcg acaggtgcgc gttgaatatc
     1201 ttcgcgatgt cgaagtactg gttacccgtc gtcaggtgga tgttcgccca gctcgggtgg
     1261 ttggggtcgt ccggcggccg agcggggtcg agacccgcat gacagctggc gcaggcgaca
     1321 ccgaacagca cgcgcgggcc gaccggcagc gggttggcga acttgcggac gccgacgatg
     1381 ccggtggcgt gcgggtcggt gcagcgatcg aacccggtcg ttccgtcgcc atcggtgcag
     1441 tcgggatcat tgatggcccc ccactgcgtg aaccgctgcg agcgcggcgt cgccagcaag
     1501 gcatcgaagc cgagccggag attgaacgga gcttccggca agatcaggct gaagaacttc
     1561 tcgccgccga acgtgctgtg tagccagacg tccctgccgt gctcctgctg cggtgtcagc
     1621 ggcggtgccg cgaccggcgg cgattcctcc tcgccccggt cacagcccat caacagcgac
     1681 aacgtgaata ccagaccaaa cgcccatctc atacccaacc tccgacctcc atgacaatcc
     1741 gcatggagaa agcacgaacc gtgccggcaa cgcgacgacg cacgcgccgg tgctgagggc
     1801 cgcgatgacc gaatggatgg ctcgtcgttc ggagcactca cgcgcgtcgt ggtgcagggg
     1861 tggactcgat cacgcgcggc cattgcccgc gctccggcgc gcggtgtgcc gcgatgcaca
     1921 agccggcctg ctgtcacatt caagtacgga tacgtcgtcg agagacatac cagcagcgct
     1981 gcaagccatc cctcctgcgg tgacctgacg cagccaaggg aacgtctgta ccggctcggg
     2041 caccggtcga gccgagggcg gtctcatcgt gagcgggtcg ggatgcgccg gcctgatctc
     2101 cgagctctca ctgcggtagc gtgagcgtca cgctgacccc gccgcccggt cgattggcga
     2161 gcgcgacgcg gccgccgtgg gcctcggcga tctcgcggac cagcgcgagc cccaggccgg
     2221 tgccgccgcg cttggtcgag tagaacggca ggagcgcgtg ggacagcacc gcctcggtca
     2281 tgccggtgcc gcgatcgctg acctcgatcg cgacggcgcc gggcagcttg cggacgcgga
     2341 gctggacctc ggcgggcggc gatccggact cgtgggcgtt gcgcagcagg ttgacgagcg
     2401 cctgctcgag ctgcgcggga tcaaagtggc cgcgggtatc ggggagcgcg ccgtcgacct
     2461 ggaacgcgac ctggccgcgc agccgctcga cgagatcggc ccatgcggtg ggctcgggcc
     2521 gcggcgccgg cagcttggcg aaccgggcgt agcccaggat gaactgctcc aggtgacggg
     2581 cgcgctcggc gatcgtcgcg aacaccttgt cgagctggtc gtgcttgccg cgcttcagca
     2641 gctcgccacc ggaatgggcg agtgaggcga tcggcgccag cgagttgttg agctcgtggc
     2701 tgatcacgcg gatcaccttc ttccaggtcc ggacctcctg gcgatggagc tccgaggtca
     2761 ggtggcggag cacgaacagc tcgtgcggcc ggccgttgag gcgaaacccg ttgcgcgcc
//
LOCUS       DASVOR010000386         3881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_500479,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000386 DASVOR010000000
VERSION     DASVOR010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3881
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500479"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1148
                     /locus_tag="VF516_07820"
     CDS             141..1148
                     /locus_tag="VF516_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107622.1"
                     /translation="MHDVKHRKRRYLFTAALGLAMLLSSLGLADSPVRPRSSGTFHKG
                     SLTMPLRRGGATPFEAFLPALPSGCEAEMTITLTWDEQANQVHARLQGRHALVPHPTI
                     RRTLGVDFFPNPIWPEQKDFTDGRYLFWILSPAQMITFYYDSATHDIMGSEFEHPTPP
                     PGAASLTVPGLKLVPSPFFQPDAEGNVDVEWSYAYNNVVRGDLPQFAHSFYTAPPANL
                     CMANPFRYDQSTTIGYTSPARPAAEALSFSDYLRNGLVFDITVEPPQYVTFPPVAGQT
                     SFYSGATVVGGGIPKGWALDLDAVFMNNAPPIKPFAAAHTCRDYFVPRHNPNINFCAQ
                     R"
     gene            1160..2530
                     /locus_tag="VF516_07825"
     CDS             1160..2530
                     /locus_tag="VF516_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107623.1"
                     /translation="MSLIKTFSYFPGSLSLLVPVALAACTNTPRSEPIGIALGESRVT
                     EGGTYNLHLEDTVRFNPIQPGSDPVRGRRLFGLAADLETSDPSQALFQGPSRAFGGTV
                     VSDGRSCFTCHRGVQATALGLPPPPLSATVPLTDPLFTGLDADAQNDPDGMHNLDELG
                     LIKYRPHRFNLARPQSDPYRQCFAWRKAIHLVNLAFASGFLSDARGRAMFEVDRGAVF
                     SHTQDSDDRFDDLFTVQDGNDLQAFQFGVLSDPRLAALRDPDDPMYTVLVNDPFYTVP
                     IATPAQRRGSEVFRKNCMPCHNTPNVFNNLSNVEALGDDTRPPNFPSFAPAVGRTFDV
                     GVAERNRHGLRFTHDDGDGQFSPIVIPLANEDGSTNLLTVTFDLGLGAITGRTEDVGR
                     FKVPQLRGVKHNAPYFHDNSAFSLEEVVDYFNSDDYNSSKDGSKFPIHLNAHERADLL
                     AFLDIL"
     gene            2613..3755
                     /locus_tag="VF516_07830"
     CDS             2613..3755
                     /locus_tag="VF516_07830"
                     /inference="COORDINATES: protein motif:HMM:NF005757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8107624.1"
                     /translation="MSIQVRSASRGSLALLVLPLALCAWLASVARAAEPEKKPGPAPG
                     SFTIENFPLESGATLPRAIVVYGTYGTLNAAKDNAVLLPSHYMAQSDGYDWLIGDGRA
                     LDPKKHFLIMTELFGNGRSSSPSNTPEPFHGPRFPVTTIRDNVEAVRRLLEDGLGIHH
                     LKAVVGFSMGAQQAFQWAVSHPKFADKIVATAGTARCWPHGVVRLDGQIRAITLDPAF
                     KGGDYTAQPVRGIETTGIVWLGWLFSQEWWKRELWKKSEPAAAGMTLTEYIEKQAKEF
                     FAGLDANDLILQMRTWQAHDVGTTPGFGGSTENALRSISVPVLYMPSETDLYFPLGDA
                     RQEARSIRKVTLAPIPSLWGHPAGVGASPEDLAFIERKLRAFLATP"
BASE COUNT          624 a         1423 c         1219 g          615 t
ORIGIN      
        1 gccggcatgt attgctccgg cgtgatcgtc gcgatctatt gatgtcgatg ttgctgtagc
       61 tgcgtggagc tttccggcgc ggcggggcag tcttgcccgc aggcagcgct gcgatgggtc
      121 gcgacgggaa ggatagctcc atgcacgacg tcaagcaccg caagcgcaga tacctgttca
      181 ctgcagcgct cggtctcgcg atgctgctca gctcgctggg actggccgac tcgcccgtgc
      241 ggcccagatc atcgggcacg ttccacaagg gctcgctgac catgccgctg cgccgcggcg
      301 gcgcgacgcc gttcgaggcc ttcctccccg cgctgccgtc cggctgcgag gcagagatga
      361 cgatcacgct gacgtgggac gagcaggcca accaggtcca cgccaggctc cagggcaggc
      421 acgcgctggt cccccacccg acgatccggc gcacgctggg cgtcgatttc ttcccgaatc
      481 cgatctggcc cgaacagaag gatttcaccg acggccgtta cctgttctgg atcctgtctc
      541 ccgcacagat gatcaccttc tattacgaca gcgccacaca cgacatcatg gggagcgagt
      601 tcgagcaccc cacgccgccg cccggcgcgg cctcgctcac cgtgcccggc ctcaagctgg
      661 tgccgtctcc gttcttccag cccgacgccg agggcaacgt cgatgtcgag tggtcgtacg
      721 cctacaacaa cgtggtgcgc ggcgacctcc cgcagttcgc gcactcgttc tacaccgcgc
      781 ctcccgcgaa cctgtgcatg gccaacccgt tccgctacga tcagtcgacg acgatcggct
      841 acaccagccc ggcgcgcccc gccgcggaag cactgtcgtt ctcggactac ctccgcaacg
      901 gcctggtgtt cgacatcacg gtcgagccgc cgcagtacgt cacgttcccg ccggtcgcgg
      961 gtcagacctc gttctacagc ggcgcgacgg tcgtcggcgg cgggatcccc aagggctggg
     1021 cgctcgatct cgacgcggtg ttcatgaaca acgcaccccc gatcaagccg ttcgcggcgg
     1081 cgcatacctg ccgcgactac ttcgtgcccc ggcacaaccc gaacatcaac ttctgcgcgc
     1141 agcgctgacg agcagtatca tgagcctaat caagacgttc agctatttcc cgggctccct
     1201 gagcctcctc gtccccgtgg cgctcgcggc ctgcacgaac acgccgcgca gcgagccgat
     1261 cggcatcgcg ctcggcgagt cacgggtcac cgagggcggc acctacaacc tccacctcga
     1321 ggacaccgtc cggttcaacc cgatccagcc cggctccgat ccggtgcgcg gccggcggct
     1381 gttcggtctc gcggccgatc tcgagacctc cgaccccagc caggcgctgt tccaggggcc
     1441 atcgcgggcg ttcggcggta cggtcgtctc cgacggtcgc agctgcttca cctgccaccg
     1501 cggcgtgcag gccaccgcgc tcggcctgcc gccgccgccg ctgtcggcga cggtcccgct
     1561 cacggatccg ctgttcaccg gcctcgacgc cgacgcccag aacgacccgg acgggatgca
     1621 caacctcgac gagctcgggc tcatcaagta ccggccccat cggttcaacc tggcccggcc
     1681 ccagagcgat ccgtaccggc agtgcttcgc gtggcgaaag gcgatccatc tggtcaacct
     1741 ggcgttcgca tcgggctttc tcagcgacgc gcgcggccgg gccatgttcg aggtcgaccg
     1801 cggcgcggtg ttcagccaca cccaggactc cgacgaccgc ttcgacgacc tgttcacggt
     1861 gcaggacggc aacgacctgc aggcgttcca gttcggcgtc ctgtccgacc cgcggctcgc
     1921 cgcgctgcgc gatccggacg atccgatgta caccgtgctg gtcaacgacc cgttctacac
     1981 cgtgccgatc gcgactccgg cccagcggcg cgggagcgag gtattccgca agaactgcat
     2041 gccgtgccac aacacaccca acgtgttcaa caacctgtcc aacgtcgagg cgctcggcga
     2101 cgacacccgg ccgcccaact tcccgtcgtt cgcgccggcg gtcgggcgga cgttcgacgt
     2161 cggcgtcgcc gagcgcaacc ggcacggcct gcggttcacc cacgacgacg gcgacgggca
     2221 gttctcgccg atcgtgatcc cgctcgccaa cgaggacggc tccaccaacc tgctcaccgt
     2281 gacgttcgac ctcggcctcg gcgcgatcac cgggcggacg gaggacgtcg ggcggttcaa
     2341 ggtgccgcag ctccgcggcg tcaagcacaa cgcgccctac ttccacgaca actcggcgtt
     2401 ctcgctcgag gaggtcgtgg actacttcaa cagcgatgac tacaactcct cgaaggacgg
     2461 gagcaagttc ccgattcacc tcaacgcgca cgagcgcgcc gatctgctcg cgttcctcga
     2521 catcctgtga cggcgtccgc aacgggatcc gccggtcgtt tcggtcgcgc gcccccaccc
     2581 gggacgcagc gcgtgtgaaa agctctgccc ccatgagcat ccaggtcagg tccgcttcac
     2641 gggggagcct cgctctcctt gtccttccgc tcgcgctgtg cgcgtggctc gcctcggtcg
     2701 cgcgcgccgc cgagccggaa aagaagcccg ggcccgcccc cggctcgttc accatcgaga
     2761 acttcccgct cgagagtggc gccacgctcc ccagggcgat cgtggtctat ggcacctacg
     2821 gcacgctcaa cgccgcgaag gacaacgccg tgctgctgcc gtcgcactac atggcccaga
     2881 gcgacggcta cgactggctg atcggagacg gccgcgcgct ggaccccaag aagcactttc
     2941 tgatcatgac cgagctgttc ggcaacgggc ggtcgtcgag cccgagcaac actcccgagc
     3001 cgttccacgg tccgcgcttc ccggtgacca ccatccgcga caacgtcgag gcggtgcggc
     3061 ggctgctcga ggacggcctg ggcatccatc acctcaaggc cgtggtgggc ttctcgatgg
     3121 gagcgcagca ggccttccag tgggcggtga gccacccgaa gttcgccgac aagatcgtcg
     3181 ccaccgccgg gaccgcccgc tgctggccgc acggcgtcgt ccgcctcgac ggccagatcc
     3241 gcgccatcac cctcgacccg gcgttcaagg gaggcgacta caccgcgcag cccgtgcgcg
     3301 gcatcgagac caccggcatc gtgtggctgg ggtggctgtt ttcccaggaa tggtggaagc
     3361 gcgagctgtg gaagaagtcc gagccggcgg ccgccggcat gaccctgacg gaatacatcg
     3421 agaaacaggc caaggagttc tttgccggac tggacgcgaa cgacctcatc ctgcagatgc
     3481 ggacctggca ggctcacgac gtggggacca cgccggggtt cggcggaagc accgagaacg
     3541 ccctccgttc catctccgtc ccggtgctct acatgccctc ggagaccgac ctctactttc
     3601 cgctggggga cgcgcgccag gaggccaggt cgatccggaa ggtcacgctc gcaccgatcc
     3661 cgtcgctgtg ggggcacccc gcgggcgtgg gtgcaagccc cgaggacctg gccttcatcg
     3721 agcgcaagct gcgcgcgttt ctcgcgacac cctgagcgag tctccaccat gttcgagcgg
     3781 atgcaggatc tggagatcga ccgcgtggtg ctctggcgcg atgccgcgtc ggggctcgag
     3841 gcgatcctgg cgatcgatga cgtcacgctc ggcccggcgg c
//
LOCUS       DASVOR010000387         4090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_383420,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000387 DASVOR010000000
VERSION     DASVOR010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4090
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383420"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /gene="uvrB"
                     /locus_tag="VF516_07835"
     CDS             <1..1504
                     /gene="uvrB"
                     /locus_tag="VF516_07835"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEX8107625.1"
                     /translation="LRRLIELQYERNDVDFHRGTFRVRGDTVEVFPAYEADTAIRIEW
                     WGDQVEAMCEIDPLRGGVKRKLDRAVIFAASHYATPEETMRRAVQTIREELKQQLELL
                     SQQGKLLERQRLEQRTMYDLESIEQMGFCSGIENYSRHLTGRVEGEPPPTLMDYFDDD
                     YLLIVDESHQTVPQIGAMYNGDRSRKETLVEFGFRLPSALDNRPLRFDEWRTRARQTI
                     YVSATPAEWELAQARGVVVEQIIRPTGLLDPEVAVRPVAHQVDDLLAEIRDRVALGDK
                     VLVTTLTKRMAEDLTEYYREIGVRVRYLHSDVDTLERIQIVRDLRRGEFDVLVGINLL
                     REGLDIPEVSLVGILDADKEGFLRSERSLIQTIGRAARNLRGKVVLYADKETDSIKLA
                     VGETNRRRAVQQAFNQEHGITPRSASRAIMDIQVAEPLPPKGRRGAQAGLAAIAGVDF
                     KKIDDLSSLRMAIDKLRGEMKQAAADLEFERAAALRDKARELEQLELQMR"
     gene            1515..2132
                     /gene="thiE"
                     /locus_tag="VF516_07840"
     CDS             1515..2132
                     /gene="thiE"
                     /locus_tag="VF516_07840"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEX8107626.1"
                     /translation="MSVRDAICGFYAVRDRDDPVLARALVGEARVLQLRIKPAGGAPD
                     AGELVRIARMARRICDDAGAALIVNDRIDVALAADADGVHLGQTDLPLAEARRIAPDL
                     WIGVSTHNLAQVRAACEAGADYLGFGPVFATTTKANPDPVQGLDGLRAAVAAAAGRPV
                     VAIGGITAARAGDVYRTGAHAICAISAVNGARDPRDAARQLARPG"
     gene            2347..2643
                     /locus_tag="VF516_07845"
     CDS             2347..2643
                     /locus_tag="VF516_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107627.1"
                     /translation="MSKLGLIASILSIAGVLTFATINASAEDAPTPCIHTDFKTELVK
                     QACTKGGQLEAKTAMKAFMKEKKIKTCNQCHEKLAPKYELKKDGLEQFQKAGGK"
     gene            complement(2697..3485)
                     /locus_tag="VF516_07850"
     CDS             complement(2697..3485)
                     /locus_tag="VF516_07850"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8107628.1"
                     /translation="MLASAALLSGCFWTTTKSEGDAMRKDITLVQSRLDTKEKALDEQ
                     IAQLHQVLEDATKLLKRNSADIGADVESLRGEVRTANGLVTAINNSIADLKQAFDTYR
                     KGNDARLDALEQRVAQIESGKPTASSSPEELWKLGTEAFKAARYNDAIDIFKRLAQTY
                     PTHDKASEAVYFRGQAYSNLKDWDHAIGVYQQLYDKYPDSQLADDGLYFAAVAAQQLK
                     NCTEARTYLDLVKKKYPKSNVTKQVNELDAALKRDLKNKAKCSS"
     gene            complement(3607..>4090)
                     /locus_tag="VF516_07855"
     CDS             complement(3607..>4090)
                     /locus_tag="VF516_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8107629.1"
                     /translation="CKACAADSECGPGGACQAGACRQTKSCTKDDQCADDEDCVNGFC
                     RKAGTTSAGDATCTLATVYFAFDDASVQPSERDRLAANVACIEKTKGKNVYLIGHTDT
                     SGTEEYNIALSERRAQSVADYMARLGTDPARMQIVPKGETEPTGLGDDKDRRVEFQWH
                     "
BASE COUNT          613 a         1411 c         1439 g          627 t
ORIGIN      
        1 gctgcgccgg ctgatcgagc tgcagtacga gcgcaacgac gtcgacttcc accgcggcac
       61 gttccgggtc cggggcgaca ccgtcgaggt gttcccggcc tacgaggccg acaccgcgat
      121 ccgcatcgag tggtggggcg atcaggtcga ggcgatgtgc gagatcgatc cgctgcgcgg
      181 cggggtcaag cgcaagctgg accgcgcggt gatcttcgcg gcgtcgcact acgccacgcc
      241 cgaagagacc atgcggcgcg cggtccagac catccgcgag gagctcaagc agcagctcga
      301 gctgctgtcg cagcagggca agctgctcga gcgccagcgg ctggagcagc gcacgatgta
      361 cgacctcgag tcgatcgagc agatggggtt ctgctcgggg atcgagaact actcgcgcca
      421 cctgaccggc cgcgtcgagg gcgagccgcc gccgaccctg atggactact tcgacgacga
      481 ctacctgctg atcgtcgacg aatcacacca gaccgtgccg cagatcggcg cgatgtacaa
      541 cggcgaccgc tcgcgcaagg agaccctggt cgagttcggg ttccggctgc cgagcgcgct
      601 cgacaaccgg ccgctgcggt tcgacgagtg gcgcacccgg gctcgccaga cgatctacgt
      661 gtcggcgacg ccggccgagt gggagctcgc ccaggccagg ggcgtggtgg tcgagcagat
      721 catccggccg accggcctgc tcgaccccga ggtcgcggtc cgtccggtcg cgcaccaggt
      781 cgacgacctg ctcgccgaga tccgcgaccg ggtcgcgctc ggcgacaagg tcctggtcac
      841 cacgctgacc aagcggatgg ccgaggacct caccgagtac taccgcgaga tcggcgtccg
      901 cgtccgctac ctgcactccg acgtcgacac gctggagcgg atccagatcg tccgcgatct
      961 gcggcgcggc gagttcgacg tgctggtcgg catcaacctg ctgcgcgagg ggctcgacat
     1021 ccccgaggtc tcgctggtcg gcatcctcga cgccgacaag gagggcttcc tgcgcagcga
     1081 gcggtcgctg atccagacca tcggccgcgc ggcgcgcaac ctgcgcggca aggtcgtgct
     1141 gtacgccgac aaggagaccg actcgatcaa gctcgcggtc ggcgagacca accggcggcg
     1201 ggcggtgcag caggcgttca accaggagca cggcatcacg ccgcgctcgg cgagccgcgc
     1261 gatcatggac atccaggtcg ccgagccgct gccgcccaag ggccggcgcg gcgcccaggc
     1321 cgggctcgcg gcgatcgccg gggtcgactt caagaagatc gacgatctca gctcgctgcg
     1381 gatggcgatc gacaagctgc gcggcgagat gaagcaggcc gccgccgacc tggagttcga
     1441 gcgcgcggcg gcgctgcgcg acaaggcgcg cgagctcgag cagctcgagc tgcagatgcg
     1501 gtaggacgct cgcgatgagc gtgcgcgacg cgatctgtgg gttctacgcg gtgcgcgatc
     1561 gcgacgaccc ggtgctcgcc cgcgcgctgg tcggcgaggc ccgcgtgctg cagctccgga
     1621 tcaagccggc cggcggcgcg cccgatgccg gcgagctggt gcggatcgcg cggatggcgc
     1681 ggcggatctg cgacgacgcc ggcgcggcgc tgatcgtcaa cgatcggatc gacgtcgcgc
     1741 tcgcggccga cgccgacggc gtgcacctcg gccagaccga cctgccgctc gcggaggccc
     1801 gccggatcgc ccccgacctg tggatcggcg tgtcgaccca caacctcgcc caggtccggg
     1861 ccgcgtgcga ggccggcgcc gattacctcg gcttcgggcc ggtgttcgcg accacgacca
     1921 aggccaaccc ggatccggtc caggggctcg acgggctgcg cgccgcggtc gccgcggccg
     1981 cgggccggcc ggtcgtcgcg atcggcggca tcaccgcggc acgggccggc gatgtctatc
     2041 gcaccggcgc gcacgcgatc tgcgcgatca gcgcggtcaa cggcgcgcgc gatccgcggg
     2101 acgccgcccg gcagctcgcg cggccgggct gagctcgcca cacttccgca cggccccgca
     2161 ctgcgacgtc gcacggcggc tccgccgaac cgtggggcat ttcgccacgc gaggcgggcc
     2221 ggcggccggc gcgggttgcc cgtggcgtcg agctagttca cgatgcccgg cacgggtccg
     2281 cccactgaat agtcgctgct gggcaccgtc gaagccagta cccgcaaccc acaaggatcg
     2341 acatcgatgt ccaagctcgg attgattgcc agcatcctgt ccatcgccgg cgtgctcacg
     2401 ttcgcaacga tcaatgcgtc ggccgaggac gcgccgacgc cgtgtatcca taccgacttc
     2461 aagaccgagc tggtcaagca ggcgtgcacg aagggcggcc agctcgaggc caagaccgcg
     2521 atgaaggcct tcatgaagga gaagaagatc aagacctgca accagtgcca cgagaagctc
     2581 gcgcccaagt acgagctcaa gaaggacggg ctggagcagt tccagaaggc cggcggcaag
     2641 tgaccgccgc gcgcggcgtc gagcgctgac cgccgcgccg gcgcgctacg tgcgcgtcac
     2701 gagctgcact tcgccttgtt cttgaggtcg cgcttgagcg cggcgtcgag ctcgttgacc
     2761 tgcttggtca cgttcgactt gggatacttc ttcttgacca ggtcgaggta ggtccgggcc
     2821 tcggtgcagt tcttgagctg ctgcgccgcg accgccgcga agtacagccc gtcgtcggcg
     2881 agctggctgt cggggtactt gtcgtagagc tgctggtaga cgccgatcgc gtggtcccag
     2941 tccttgaggt tgctgtaggc ctggccgcgg aagtacaccg cctcactggc cttgtcgtgg
     3001 gtcggatagg tctgggccag ccgcttgaag atgtcgatcg catcgttgta gcgcgcggcc
     3061 ttgaacgcct cggtgccgag cttccacagc tcctcgggcg acgagctcgc ggtcggcttg
     3121 ccgctctcga tctgggcgac ccgctgctcg agcgcgtcga gccgcgcgtc gttgcccttg
     3181 cggtaggtgt cgaacgcctg cttgaggtcg gcgatcgagt tgttgatcgc ggtgaccagc
     3241 ccgttggcgg tccggacctc gccgcgcagc gactcgacgt cggcgccgat gtcggcgctg
     3301 ttgcgcttga gcagcttggt cgcgtcctcg agcacctggt ggagctgggc gatctgctcg
     3361 tcgagcgcct tctccttggt gtcgagccgg ctctggacca gcgtgatgtc cttgcgcatc
     3421 gcatcgccct cgctcttggt cgtggtccag aagcagcccg acagcagcgc cgccgacgcg
     3481 agcaaggacc cgagccttga cccgaacgac gaacgaatca accgaaggcc gcgcaaccac
     3541 cagtcgtgct tcatcacgag tgcctcctcg cgcgcattat ccacggctgc gcgcggcgat
     3601 gcgggctcag tgccactgaa actccacgcg gcgatccttg tcgtcgccca ggccggtcgg
     3661 ctcggtctcg cccttaggga cgatctgcat ccgcgccggg tcggtgccga gccgcgccat
     3721 gtaatcggcg accgactggg cgcggcgctc ggacagcgcg atgttgtact cctcggtgcc
     3781 cgacgtgtcg gtgtggccga tcaggtagac gttcttgccc ttggtcttct cgatgcaggc
     3841 gacgttggcg gcgagccggt cgcgctcgct gggctggacc gacgcgtcgt cgaatgcgaa
     3901 atacacggtc gccagcgtgc aggtcgcgtc gccggcgctg gtcgtgccgg ccttgcggca
     3961 gaacccgttg acgcagtcct cgtcgtcggc gcactgatcg tccttcgtgc acgacttggt
     4021 ctgccggcac gcgccggcct ggcacgcgcc gccggggccg cactcgctgt cggccgcgca
     4081 cgccttgcac
//
LOCUS       DASVOR010000388         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208218,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000388 DASVOR010000000
VERSION     DASVOR010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208218"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1251)
                     /locus_tag="VF516_07860"
     CDS             complement(67..1251)
                     /locus_tag="VF516_07860"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX8107630.1"
                     /translation="MQNSITMRAVPVVLVCSLAAAGCDATLAESTASAPVIGGSDAAP
                     GKWPDVAAVMFPIDGEVPLCTGTLVAPTVVLTAGHCYDRTSAPLPDSVLIGTASLDRP
                     GDGETIPIARGYVYPNAGTTEDLAVLVLARPSTRPPRRIVDGWARADLVNGAAISLVG
                     FGAIDRAGMVYVDQLQEATTTITDVDCAASPGCNPTAQPAGELGAGGMGVDTCPGDSG
                     GPLYLSTGYGPVLAGVTARSYDNARFPCSEGGIYVRPDKVLDWIEQVAGVPVARVASP
                     TASMVATQDHCGSTRIAVNDPASQSHSFRVTTPPAHGRASVDPDGTVRVCTDAGAPDD
                     DHVVVTISDDAASVDRSLPVTIPVTAAGCCDAGGRPGGAIPLAIGVLALARRRRPRGP
                     RG"
     gene            1434..>2068
                     /locus_tag="VF516_07865"
     CDS             1434..>2068
                     /locus_tag="VF516_07865"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8107631.1"
                     /translation="MLVVTACGHSPKLGKAPPPQAAIDVERYREQVEAQVQPLLDAEL
                     VSGLVVGLYDVGKTEIYGFGKGPAQGKSQGPPDGNTLFELGPITTVYTALLLADAVQR
                     REVALDTPVSELLPLGVTMPIRDKVAITLKHLALHAAGLPPHPPSIVARGAAPDPFAG
                     YGENELYNDLLRTELIAPPGTQINYSPYGAGLLGFVLGRKIGPGYAKALEAR"
BASE COUNT          270 a          758 c          767 g          273 t
ORIGIN      
        1 aagggtaacc gacggccggt cgcgattccg gcgtccgggc caggatcccg ggcccaggat
       61 cgaccgctat ccccgcggac cgcgcggccg ccgacggcgc gcgagcgcga gcacgccgat
      121 cgccagcggg atcgccccgc ccgggcggcc gccggcgtcg cagcagcccg cggccgtgac
      181 cgggatcgtg accggcagcg agcggtcgac gctggccgca tcgtcgctga tcgtgacgac
      241 cacgtggtcg tcgtccggtg ccccggcgtc ggtgcagacc cgcaccgtgc catccggatc
      301 gacgctggcc ctgccgtgcg ccggcggggt cgtgacccgg aacgagtgcg actggctcgc
      361 cgggtcgttg acggcgatcc gggtggagcc gcagtggtcc tgggtcgcga ccatggacgc
      421 ggtgggcgac gcgacccgcg cgaccggcac gccggcgacc tgctcgatcc agtcgaggac
      481 cttgtcgggg cggacgtaga tgccgccctc ggagcacggg aaccgcgcgt tgtcgtagga
      541 ccgggcggtg acgccggcga gcaccgggcc gtagccggtc gacaggtaca gcgggccgcc
      601 cgagtcgccc gggcaggtgt cgacgcccat gccgccggcg ccgagctcgc ccgccggctg
      661 cgcggtcggg ttgcagccgg gcgatgccgc gcagtcgacg tcggtgatgg tggtggtcgc
      721 ctcctggagc tggtcgacgt acaccatgcc ggcgcgatcg atcgcgccga aaccgaccag
      781 cgagatcgcg gcgccgttga ccagatcggc gcgcgcccag ccatcgacga tccggcgcgg
      841 cgggcgggtc gacggccggg ccaggacgag caccgcgagg tcctcggtgg tgccggcgtt
      901 cgggtagacg tagccgcgcg cgatcgggat ggtctcgccg tcgccgggcc ggtccagcga
      961 ggcggtgccg atcagcacgc tgtcgggcag cggcgcgctc gtccggtcgt agcagtggcc
     1021 cgcggtcagc acgacggtgg gcgcgaccag cgtgccggtg cacagcggca cctcgccatc
     1081 gatcgggaac atgaccgccg caacgtcggg ccacttgccg ggcgcggcgt cggagccgcc
     1141 gatcaccggc gcgctcgcgg tcgattccgc gagggtcgcg tcgcatcctg cggcggccag
     1201 cgaacagaca aggacgaccg ggaccgcgcg catggtgatc gagttttgca tgtcggctgc
     1261 ggcgcggcga tgcatctcgc tgcaacctcc tgccgcaggt gtggattgcg cgccgtgcgc
     1321 ggtcggggca gtctacgcag cgtcccatgt ctggccggtt gatctcccgg ggtgctttgc
     1381 cccccgcccc gctgtgctcg caccttcgct tcgtcgcgcc cacagcggtc gccatgctgg
     1441 tcgtcaccgc ctgtggccat tcgcccaagc tcggcaaggc gccgccgccg caggccgcga
     1501 tcgatgtcga gcgctaccgc gagcaggtcg aggcccaggt ccagccgctg ctcgacgccg
     1561 agctggtgtc cgggctggtc gtcgggctct acgacgtcgg caagaccgag atctacggct
     1621 tcggcaaggg gccggcccag ggcaagagcc agggcccgcc cgacggcaac acgctgttcg
     1681 agctcggccc gatcaccacc gtgtacacgg cgctcctgct cgccgacgcg gtgcagcgcc
     1741 gcgaggtcgc gctcgacacc ccggtctcgg agctgttgcc gctcggcgtc acgatgccga
     1801 tccgcgacaa ggtcgcgatc acgctcaagc acctggcgct gcacgccgcg gggctgccgc
     1861 cgcacccgcc gagcatcgtc gcccgcgggg ccgccccgga tccgttcgcc gggtacggcg
     1921 agaacgagct gtacaacgac ctgctgcgca ccgagctgat cgccccgccg ggcacccaga
     1981 tcaactactc gccgtacggc gccggcctgc tcgggttcgt gctcggccgc aagatcggcc
     2041 ccggctacgc caaggcgctg gaggcccg
//
LOCUS       DASVOR010000389        11630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_62045,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000389 DASVOR010000000
VERSION     DASVOR010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11630
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62045"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /gene="tnpB"
                     /locus_tag="VF516_07870"
     CDS             complement(<1..215)
                     /gene="tnpB"
                     /locus_tag="VF516_07870"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX8107632.1"
                     /translation="MIAHGLPIYVALEPVDMRLGAERLEALIRERMRAEPRSRALFVF
                     VGKRGHSMKVLTWDGTGTIVVHKKLDAG"
     gene            complement(212..568)
                     /locus_tag="VF516_07875"
     CDS             complement(212..568)
                     /locus_tag="VF516_07875"
                     /inference="COORDINATES: protein motif:HMM:NF024412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8107633.1"
                     /translation="MRKHYTEAQRTALVELVTTGRTTPREAAAQLGVSESTAYYWLKR
                     ARRRPALALVRQVARPGSAARLRTEAPTFARLVRATEAASAITLRVAGTEIEVRPGFD
                     RALLRDVISALVEGAP"
     gene            complement(995..1708)
                     /locus_tag="VF516_07880"
     CDS             complement(995..1708)
                     /locus_tag="VF516_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107634.1"
                     /translation="MNLWDCGSEKNSSHFTAVRILKYLLVHRAEASKEGQGYVDIARM
                     SGLFEEVFDAREDFIRTLERLVSRQLVETNTRSTEGVLGASHVRATSAGWYFSRHLRQ
                     RFPYLDLVLQDTPIASSSVERSLRDLVEQVDNLGDREEQKIERLGIRFRRVKTFLEYL
                     EDEERAEAKRYSLSGRIDILSERIVKPMADALEEEREWILTRVRENRERYADEPQIDD
                     VEDKQFIGDRLAADDDVEG"
     gene            1757..2419
                     /locus_tag="VF516_07885"
     CDS             1757..2419
                     /locus_tag="VF516_07885"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEX8107635.1"
                     /translation="MILPIVMYHGTTSWPEPRSFDALLDVPSPLRPAVEPYLVRFTYL
                     LHDLSEISDEELRDGAMRTALAKLVSMCFKHARTHADFLQILGRWMGVAREVMRAPHG
                     LEALAQVMRYILEVNEHVEPEELQGLLERELGPEAKDAIMTAGQRLIEQGRQQGIQQG
                     IQQGIQQGAQGVLLRLLRQRFGDAVNALVEQRIATASVEQIEAWTGRVLSAATLAEVI
                     AD"
     gene            complement(2500..2864)
                     /locus_tag="VF516_07890"
                     /pseudo
     CDS             complement(2500..2864)
                     /locus_tag="VF516_07890"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
     gene            complement(2919..3128)
                     /locus_tag="VF516_07895"
     CDS             complement(2919..3128)
                     /locus_tag="VF516_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8107636.1"
                     /translation="MVAVRPLGPSKHAVALATWHHLPLRVRAAVPLSDDELFELCQVN
                     RDLQIERTSDGEIWVMPPAGGETGR"
     gene            complement(3165..3581)
                     /locus_tag="VF516_07900"
     CDS             complement(3165..3581)
                     /locus_tag="VF516_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107637.1"
                     /translation="MLYLGTCHCEAIGFAYETAIPPQAWPLRACACRFCRCHGAATTS
                     VPHGALQLTCRDVDQLLRYRSGLRTADFCVCRSCGVYLAAITLDGRFGIINTNDLIDH
                     ALPLSAPPLPMSYDGETASARTARREKRWTPVRAAT"
     gene            complement(3801..4166)
                     /locus_tag="VF516_07905"
     CDS             complement(3801..4166)
                     /locus_tag="VF516_07905"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8107638.1"
                     /translation="MVRYLVRDIDRALAFYTQHLGFRVAMQSGTVFAMITRGDMHLIL
                     SGPGTSGARPMPDGRQQEPGGWNRIVLYVENLDATIARLQAAGARFRNQVEVGPGGKQ
                     IQVDDPDGNPIELHEAPGR"
     gene            complement(4359..5198)
                     /locus_tag="VF516_07910"
     CDS             complement(4359..5198)
                     /locus_tag="VF516_07910"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8107639.1"
                     /translation="MTTEFEQHRSFLFGLAYRILGSVTDAEDIVQDAFLRWRETDHST
                     IEQPRAYLARVVSRLCLDRMKSATARREHYVGMWLPEPLVTEPAAPLAEDLSVALLLT
                     LERLSPLERAAFLLHDVFDMDYADVAAALERSEAACRQLAARAREHVRDARPRFAADT
                     DAQTKLADAFHAALATGDLSTLAQLLADDAVLYSDSGGKRFVAYHPIHGKDKILKIFT
                     RAAGKRAIPRPDQIERTWINGMPGFIVRTAEAIETIALESNGDRIAALYIVANPDKLK
                     HLA"
     gene            complement(5370..6326)
                     /locus_tag="VF516_07915"
     CDS             complement(5370..6326)
                     /locus_tag="VF516_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8107640.1"
                     /translation="MKRFLKRLAIGVAWFLVLGTATVAVAVVVREDRRFDAPYPDIHA
                     SMDPAVIARGRYLVTGPAYCTACHGQLDPASRSPVLETEPHLAGGLALHLPIGTIYAR
                     NITPDKATGIGTYTDPEIARVLRYGVDPSGRVTMPFMPFTDMSDDDLAAIVSYLRSQP
                     PIDHPVPASNYNMRGRAARAFLIEPRGPTGPIRAHVGRGPTVEYGQYLANSVANCGGC
                     HTKSNRRTGAAEGVSFAGGSTVDSRTSPGTKFVTPNLTPDPKTGHIYAWSEDVFVARF
                     RTAVPTASPMPWGNFKNLSEDDLRALYRYLRSLPPAKHGQEL"
     gene            complement(6433..7311)
                     /locus_tag="VF516_07920"
     CDS             complement(6433..7311)
                     /locus_tag="VF516_07920"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8107641.1"
                     /translation="MDRLATIEAFVEAAERGSFTLAARHLRLSPSALSRRIAHLEGEL
                     GVRLFHRTTRALRLSDDGRAFFERARDALRELAEAASATAGLRDRPAGLLRVEAPTIL
                     GRHVLVPAIQRLIARHPDVRVELALRDHALDLASHGIDVAIRLGAQPDSALVARRIGT
                     ARMGVYGAPGYFRRAGKPRTVRDLERHELLGFALHGRVVPWRLRDGDTVRELVPSQRI
                     VVDSADALVDLAASGAGLAWLCSFMMLRQPVVEVLRDAACAESAVHVVSLPSRHVLPK
                     VRVFVQLVEAQLARAI"
     gene            7388..8449
                     /locus_tag="VF516_07925"
     CDS             7388..8449
                     /locus_tag="VF516_07925"
                     /inference="COORDINATES: protein motif:HMM:NF025279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HEX8107642.1"
                     /translation="MPSSHRLLTTAVVTLILAAAHHIYGGIIYATPWRVHGAFVALVL
                     AGVIAWLHHRRAYGLELTLIAIACVLGVGLFEGLYNHVVKNALYLAGAPRGVVLRMFP
                     PPTYEPPGDPVFELSGIGEALAGGVTAVAALRFAAARRGAGTRLAVGTALAPRALVSV
                     TGEPVPLPDPVWLTHLQFRRFAGCPICNLHLHRFQTRHAELLGAGIHEVVVFHSRADE
                     LRTHTSGLPFAVIADPDKRLYAAFGVEAGARSLLDPRVWPTIIRAVARSTVAILRGRE
                     RAPSVVQRAGRLGLPADFLIDSDGRILACKYGDHADDQWSVDEVLAAAHGASDEPSAA
                     SPRRAVESNSGLPSTQVLP"
     gene            complement(8522..8935)
                     /locus_tag="VF516_07930"
     CDS             complement(8522..8935)
                     /locus_tag="VF516_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107643.1"
                     /translation="MLYLGTCHCEAIGFTYETAIPPRAWPLRACACRFCRCHGAATTS
                     DPHGALQLACRDVDQLLRYRFGLHTADFCVCRACGVYLAAITLDGQFGIINTNALVDR
                     SLSLAAPLPMSYDGETASTRTARREQRWTPVRAST"
     gene            complement(9024..10601)
                     /locus_tag="VF516_07935"
     CDS             complement(9024..10601)
                     /locus_tag="VF516_07935"
                     /inference="COORDINATES: protein motif:HMM:NF025746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4419 domain-containing protein"
                     /protein_id="HEX8107644.1"
                     /translation="MVTFRVSDVECERTRLPTRSTASQLAKLGWRPEAHGIAATELVG
                     NPHVHPLALAVHLAFAGHRPLVLTPDAVWLCIAQSLATHIAQNAEALRSRLVRHAGKL
                     ALDVRRDDFVPGDPANDWPGVVDNLVAQIRGHIGGRADLFVANFSTTGPLDRIASQIA
                     LLGAMREYFSYSVMTMCGIPEITLTGTPEDWASIRRRAAAFQEFDLAWWVEALDPVLA
                     QLEATARGHVDREFWRRLYKLEIASGGDRIYGWLNTLFAYVGDPPQRNTFPTLDDGPF
                     EGNCMDDLPHGRTSVPFDWNVLGERVEMELVGGLWGVTQDPQGALGVASGWLVSLSRT
                     AREGSRAGGGGSGGRSSGPSASIGHGTGQPRTPRPLSLRDAGTVETLAALRDAACDDP
                     AELSLPRTPRLRSLDGIQHLRRLVALSIAEAPLLETIAPLAGMASITDLYLGGCPRLT
                     NVGPVLATLPRLESLSLARISGLQLADVLPIVRMHRLRRISLVDCTGLPEHIRYLYQE
                     DAVLDLQAEIARLAGLG"
     gene            10684..11448
                     /locus_tag="VF516_07940"
     CDS             10684..11448
                     /locus_tag="VF516_07940"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8107645.1"
                     /translation="MTLFDLTGKVALVTGSTKGIGKAIAERLAEHGARVVVSSRKADA
                     CNQVAAGINAARGAQVAVPIPCNINYKDQLRALVDETHEKLGPIDVLVCNAALNPAYG
                     SMSQIPDDAFDKILGANIRSNHWLAQMVLPDMIAKRDGAIIIVSSIGGYRGSTTIGTY
                     CISKAADLQLARNLAVENGPHNIRVNCIAPGLVKTDFARALWDTEEGERRASSGTPLR
                     RLGEPDDIAGAAVFLASRAGAWMTGQSIIVDGGALA"
BASE COUNT         1862 a         3899 c         4057 g         1812 t
ORIGIN      
        1 ccggcgtcga gcttcttgtg aacgacgatc gtcccggtgc catcccaggt caggaccttc
       61 atgctgtggc cgcgcttgcc cacgaacacg aacagcgcgc gagagcgcgg ctcggcccgc
      121 atccgctcgc ggatcagcgc ctcgagccgc tcggcgccga gtcgcatgtc gaccggctcg
      181 agtgccacgt agatcggcag cccgtgcgcg atcacggggc gccctcgacc agcgccgaga
      241 tgacgtcgcg cagcagcgcc cggtcgaagc ccggtcgcac ctcgatctcg gtaccggcga
      301 cgcgcagcgt gatcgcgctc gccgcctcgg tcgcgcgcac cagccgtgca aaggtgggcg
      361 cctccgtgcg caatcgggcc gcggaacctg gacgcgccac ctgtcgaacc agcgcaagcg
      421 ctggccgccg cctggcgcgc ttgagccagt agtaggccgt cgactcggac acgcccagct
      481 gtgcggccgc ctcccggggc gtggtccggc ccgtagtcac cagctcgacc agcgcggtgc
      541 gctgggcctc ggtgtagtgc ttcctcatcc gtcgagggtc ggtaccgccg ctgcgtctcg
      601 ctacgtgggg gcgatgcagc gcttacctac gttggcatct ttgctgcgtg ccggtcaggc
      661 ctcaatgtgg ggtcgacgca tcgaataccc ggagagcgcc cgggcggatt caagtcgctc
      721 tcccgacgat tctagcttgc acccagcggc ttacagagcg ccagggcgac gcgtctgcga
      781 gtgccccggc attccgctcc ggtacccccc accgggaagc tgcgccggcc ttgggatcgc
      841 gattgatttc gcagacggcg ggatcggtcg gccgatctaa cgcggctcgc tacgagtcca
      901 agccagcacg gcccgccgat tatcttcgct ggctacggcg acgaagcttc cgggcaccag
      961 aaatctcttg agagcaatga cttcctacga actctcagcc ttcgacgtcg tcgtctgccg
     1021 cgagtcgatc gccgataaac tgtttatctt ccacgtcatc gatctgtggt tcgtccgcgt
     1081 atcgttcgcg gttttcgcgc acacgggtaa ggatccattc gcgttcttcc tcgagcgcat
     1141 ccgccatggg tttgacgatt cgctcgctaa gaatgtcgat gcgtccactc aaagaataac
     1201 gtttcgcctc cgctcgctct tcgtcctcaa gatattcaag aaacgtcttg acgcgccgga
     1261 agcgaatccc cagtcgttct attttctgct cttctcgatc gccaagattg tctacctgtt
     1321 ccaccagatc gcggagagat ctctccacag aagagctagc gataggggta tcttgcaaaa
     1381 cgagatcgag ataaggaaac cgctggcgaa ggtgccgtga gaagtaccaa cctgcagagg
     1441 tcgcgcgaac atgactagcg cccagaacgc cttccgtaga acgtgtgttt gtttcaacaa
     1501 gttgacgcga aaccagccgc tctaatgtac ggatgaagtc ttctctcgca tcgaatacct
     1561 cctcaaatag gccactcatc cgcgcaatgt caacataccc ttggccttcc tttgatgctt
     1621 cagcgcgatg cacgagcaga tatttcagga ttcgaactgc agtgaagtgg ctcgaattct
     1681 tttctgatcc acagtcccaa aggttcatga tgggactctg cgactccttg tagtacctgc
     1741 ggtcagcacg ctgccgatga tcctgccgat cgtgatgtat cacggcacga cgtcgtggcc
     1801 ggagccccgg tcgttcgacg cgctgctcga cgtgcccagc cccctgcggc ccgcggtgga
     1861 gccctacctg gtccggttca cctacctgct tcacgatctc tcggagatct ccgatgagga
     1921 gctgcgcgac ggtgcgatgc gaacggccct cgccaagctg gtctcgatgt gcttcaagca
     1981 cgcgcgaacg cacgcggact tcctccagat cctcggccgc tggatgggcg tcgcgcgcga
     2041 ggtgatgcgg gccccgcacg ggctggaagc gctggcccag gtgatgcgct atattctcga
     2101 ggtcaacgag catgtggaac ccgaggagct gcagggactt ctggaacgcg agctcggacc
     2161 cgaagccaag gacgccatca tgaccgccgg acagcgactc atcgaacaag gacgccagca
     2221 agggatccag caagggatcc agcagggcat ccagcaaggg gcccagggag tgctcctgcg
     2281 gctgctgcgg cagcgcttcg gcgatgcggt gaatgccctg gtcgagcaac gcatcgcgac
     2341 cgcatcggtc gagcaaatcg aagcctggac ggggcgcgtg ctgtcggccg ccacgctggc
     2401 cgaggtgatc gccgactgaa ccaccagagc accgagatcg acagcgccgt gtcggttggt
     2461 ggcggtcggg acggcacgcc gctgttcgag cgtgcgtagc taccagatcc ggcggagatc
     2521 cagcacgaag ccgcgcagca ccggatcgcc ggtgacggtc accggtgcgt cgagcgtctc
     2581 gacctcggca ccggagcgat agatgtggac gcggcgctcg accggatcga tcagccagcc
     2641 gagcctggcg ccgcaggcgc gatagtcctc catcttggcc cgcaggttgc caagctcatc
     2701 ggacgcgaac gcagctcgac gacgaagtcg gggcagagcg gcgggaactt ggcgcgatcc
     2761 tcggcgctca gcgcttgcca gcacgtcagc gagatccacg cggcatcggg agagcgcgcc
     2821 gcgccatttg gcaagaggaa gccggtcgat gaatcgaagc acacgccgct gccgtcgagc
     2881 tcgctccagc gaccgagctg gacgttcagc gaagagtttt atcgtcctgt ctctcctccc
     2941 gccggcggca tgacccagat ctcgccgtcg gaggtgcgct cgatctgcag gtcgcgattg
     3001 acctggcaga gctcgaacag ctcgtcgtcg ctgagcggca ccgccgcgcg aacgcgcagc
     3061 ggcaagtgat gccaggtcgc cagcgcgacg gcatgcttgc tcggaccgag cggtcgtact
     3121 gcgaccatgg ctccatggta gcacgccggc gaacgtcaac gagctcaggt ggctgctcgt
     3181 accggcgtcc agcgcttctc gcgtcgcgca gtccgggccg aggcggtctc gccgtcgtag
     3241 gacatcggta gcggcggggc cgagagcggc aacgcgtgat cgatcaagtc gttggtgttg
     3301 atgatgccga accggccgtc gagggtgatc gcggcgagat agacgccgca gctgcggcac
     3361 acgcagaagt cggcggtgcg cagcccggag cggtagcgga gcagttggtc gacgtcgcgg
     3421 caggtgagct gcagggcgcc atggggaacc gacgtcgtcg cggcgccgtg gcagcggcag
     3481 aaccggcacg cacacgcccg cagcggccat gcctgcggcg gaatggcggt ctcgtaggcg
     3541 aagccgatcg cctcgcagtg gcaggtcccg agatagagca tgagctccca cggcgctcgc
     3601 ccacgagggc gcgcggatcc tcgaggaggg cttcgcctac cgcgccggcg acatcggccg
     3661 caacctcctc gacaagaaga ccggcgcggt caccggccag gaggacgcgc tggtcgagac
     3721 cgagaccatg tgcgtcggtg gcggccaggg cggcgcggcg ctgttcgagc gcgcgtagcc
     3781 ggcgatcgcc atcgcggccg ctacctgccg ggtgcttcgt ggagctcgat ggggttcccg
     3841 tcggggtcgt cgacctggat ctgcttgcca cctgggccga cctcgacctg gttccgaaac
     3901 cgcgctcccg ccgcctggag ccgcgcgatc gtggcgtcga ggttctccac gtagagcacg
     3961 atcctgttcc atccgcctgg ctcttgctgg cggccgtcgg gcatgggtcg agccccggag
     4021 gtgcccgggc cgctcaggat cagatgcatg tcacctcggg tgatcatcgc aaatacggtt
     4081 cccgactgca tcgcgacccg gaatcccagg tgctgggtgt agaacgccag cgcgcggtcg
     4141 atatcccgca ccaggtagcg gaccactgcc gtatccgccg agaatgatcc tgatggctgt
     4201 gggcgtcgcg gctcactcac tgccgaacct ccatgtgcat tgcagccgtg gagcaccagg
     4261 acgaacgcgc tcgccacgac cgagtgaagt actgctttga gcataccagc ctccttcgca
     4321 cccacctgcg tgtgtcacat gggcctgtgg ccacgtcctc aggcgaggtg cttgagcttg
     4381 tctggattgg cgacgatgta gagcgccgcg atacgatcac cgttgctctc gagcgcgatc
     4441 gtctcgatgg cttcggcggt gcgaacgatg aagccgggca tgccgttgat ccacgtccgc
     4501 tcgatctgat cgggtcgcgg gatcgcgcgc ttgccggcgg cgcgggtgaa gatcttgagg
     4561 atcttgtcct tgccgtggat cgggtggtac gcgacaaacc gcttgccgcc gctgtcggag
     4621 tagaggaccg cgtcatcggc gagcagctgt gcgagcgtcg agaggtcgcc cgtcgcgagg
     4681 gcggcatgga acgcgtcggc gagcttggtc tgcgcgtcgg tatcggcagc gaagcgcggc
     4741 cgggcatcgc gaacgtgctc gcgggcgcgg gcagcgagct ggcggcacgc cgcttcactg
     4801 cgctcgagcg cggcggcaac gtcggcgtag tccatgtcga atacgtcgtg gagcaggaac
     4861 gcggcacgct cgagcgggga caggcgctcg agcgtgagca gcagggcgac cgagaggtct
     4921 tcggcgagcg gcgccgcggg ctcggtgacg agcggctcgg gcaaccacat gccgacatag
     4981 tgctcgcggc gcgcagtcgc ggacttcatg cgatcgagac agagccgtga gacgacgcga
     5041 gcgaggtacg cgcgcggctg ctcgatggtc gagtgatcgg tctcgcgcca gcgcaggaac
     5101 gcgtcctgca cgatgtcttc cgcgtcggtg accgagccga ggatgcggta cgcgagcccg
     5161 aagagaaacg atcggtgctg ctcgaattcg gtggtcacgc ggcggccctg ggacggaagt
     5221 tgggtgggat catcatcatg acctcatgac gaggtggccg gggcggttgt gacatgaggg
     5281 gccgcgcgtg caagaactgc gcgcgggacg agcgaggcga tcgccgcgcg cctgacttcg
     5341 cccgaagcgc cggccaccgg atcgtcacgt cacagctcct ggccgtgctt cgccggcggc
     5401 agcgagcgga ggtatcggta cagcgcgcgc aggtcgtcct cggagaggtt cttgaaattg
     5461 ccccacggca tcgggctcgc ggtcggcacc gcggtcctga agcgcgcgac gaacacgtcc
     5521 tcgctccacg cgtagatgtg gcctgtcttc ggatccggcg tgaggttcgg cgtgacgaac
     5581 ttcgtgcccg gcgaggtgcg cgagtcgacg gtcgacccgc ccgcgaacga gacgccttcc
     5641 gctgcgcccg tgcgcctgtt gctcttcgtg tggcaaccgc cgcagttcgc gaccgagttc
     5701 gccaggtatt gaccgtattc gacggtcggt cctcgcccca cgtgcgcgcg gatcggcccc
     5761 gtcggtccgc gcggttcgat gaggaatgcc ctggccgcgc gcccgcgcat gttgtagttg
     5821 ctcgccggca ccgggtgatc gatcggcggc tggctgcgca ggtacgacac gatcgccgcc
     5881 agatcgtcat cgctcatgtc cgtgaacggc atgaacggca tcgtgacgcg gccgctcgga
     5941 tccacaccgt agcgcagcac gcgcgcgatc tccggatcgg tgtacgtacc gatgcccgtc
     6001 gccttgtcgg gtgtgatgtt gcgcgcgtag atcgtgccga tcggcaggtg gagtgcaaga
     6061 ccgccggcga ggtgcggctc ggtctcgagc acgggcgatc tcgacgccgg atcgagctgg
     6121 ccgtggcagg ccgtgcagta ggcagggccg gtcacgaggt accgcccgcg ggcgatgacc
     6181 gcggggtcca tgctggcgtg aatgtcggga taaggagcgt cgaacctgcg gtcctcgcgc
     6241 acgacgaccg cgacggcgac cgtggccgtg ccaagcacca gaaaccacgc gaccccgatg
     6301 gcgaggcgct tcaggaatct cttcatgccg ccacaagacg acacagccga ctcgattgtg
     6361 acatgcctcg cgtgacggct gtgatcagcc gccgatcggg gtcgttccca ccgctgcgcg
     6421 accgtgcgcc ggtcagatcg cccgtgcgag ctgggcctcg acaagctgga cgaacacccg
     6481 caccttggga agcacgtggc gcgacggcag cgacacgacg tggaccgcgc tctcggcgca
     6541 cgccgcgtcg cgtagcacct cgaccactgg ctggcgcagc atcatgaagc tgcacagcca
     6601 ggccagaccg gcgccgctcg cggccagatc gacgagcgca tcggcgctat cgacgacgat
     6661 gcgctggctc ggcaccagct cgcggacggt gtcgccgtcg cgaagccgcc acggcacgac
     6721 gcggccgtgc agcgcgaacc cgagcagctc gtggcgctcg aggtcgcgca ccgttcgcgg
     6781 cttgcccgcc cgccggaagt agcccggcgc accatacacg cccatccggg cggtgccgat
     6841 cctgcgggcg acgagcgccg agtcaggctg ggcgccgagc cggatcgcga cgtcgatgcc
     6901 gtgcgacgca aggtcgagtg cgtgatcgcg cagcgcaagc tcgacccgca cgtcggggtg
     6961 gcgcgcgatc aggcgctgga tcgcgggcac gagcacgtgg cggccgagga tcgtcggcgc
     7021 ctcgacccgc agcagccccg ccggccggtc gcgcagcccg gcggtcgcgc ttgcggcctc
     7081 tgcgagctcg cgcagcgcat cgcgcgcccg ctcgaagaac gctcggccgt catcggacag
     7141 ccgcagcgcg cgcgtcgtgc ggtggaacag gcgcacgccg agctcaccct cgagatgcgc
     7201 gatccgccgg ctcagcgccg acgggctgag ccgcaggtgg cgcgcggcca gcgtgaagct
     7261 gccgcgctcc gcagcctcga cgaacgcctc gatggtggcc aaccggtcca ttgttgcatg
     7321 ctatgcacag atgtcgtgcg tccgcgagta tggcgcgcgc tgggcgcaac gaccagcctg
     7381 atcgacgatg ccttcctctc atcgcctcct gaccaccgct gtcgtcacgc tgatcctcgc
     7441 tgctgcccac cacatctacg gcggcatcat ctacgccacg ccgtggcggg ttcacggtgc
     7501 cttcgtcgcg ctcgtgctgg ccggcgtgat cgcctggctg catcaccgcc gcgcctacgg
     7561 cctcgagctc acgctgatcg cgatcgcgtg cgtgctcggc gtcgggctgt tcgagggctt
     7621 gtacaaccac gtggtgaaga acgcgctgta cctggccggc gcgccgcgcg gtgttgtgct
     7681 ccgcatgttc ccgccaccga cctacgagcc gcccggagat ccggtgttcg agctcagcgg
     7741 gatcggcgag gcgctcgctg gcggcgtcac cgcggtcgcc gccctgcgct tcgccgcggc
     7801 acggcgcggc gccggtacac ggctcgcggt cggcaccgcc ctcgccccgc gtgcgctcgt
     7861 ctcggtgacc ggcgagcccg tcccgttgcc cgatccggtg tggctcaccc acctgcagtt
     7921 ccgccggttc gccggctgtc cgatctgcaa cttgcacctg cacaggttcc agacccggca
     7981 cgccgagctg ctcggtgccg gcatccacga ggtcgtggtg ttccactcgc gcgccgacga
     8041 gttgcgcacg cacacgagcg gactgccgtt cgccgtgatc gccgatcccg acaagcggct
     8101 ttacgcggca ttcggcgtcg aggctggcgc gcgctcgctg ctcgatccgc gggtgtggcc
     8161 gacgatcatt cgcgcggtcg cgcgcagcac agtggcgatc ctgcgcggcc gcgagcgcgc
     8221 cccgtcggtc gtgcagcgcg ccggccggct cggcctgccg gcggacttcc tgatcgacag
     8281 tgacggtcgc atcctcgcct gcaagtacgg cgaccacgcc gacgaccagt ggagcgtcga
     8341 cgaggtgctc gccgcggccc acggcgcgtc agatgagccg tcggcggcgt cgcctcggcg
     8401 tgcggtcgag tcgaacagcg gcctcccgtc gacgcaggta ctgccctgac cctgatcgtg
     8461 cggaaaccac cagggcgccg agattgaccg cgccgtgcca gctcgtcgaa cgtcaacgag
     8521 ctcaggtgct tgctcgtacc ggcgtccagc gctgctcccg gcgtgcggtt cgcgtcgagg
     8581 cggtctcgcc gtcgtaggac atcggtaacg gtgccgcgag cgacagcgag cgatcgacca
     8641 gcgcgttggt gttgatgatg ccgaactggc cgtcgagggt gatcgccgcg aggtagacgc
     8701 cgcaggcgcg acatacacag aagtcggcag tgtgcagccc gaagcggtag cgcagcagct
     8761 ggtcgacgtc gcggcaagcg agctgcaggg cgccatgcgg gtccgacgtc gtcgccgcgc
     8821 cgtggcagcg acagaaccgg cacgcgcacg cgcgcaatgg ccatgcccgg ggaggaatgg
     8881 cggtctcgta ggtgaagccg atcgcctcgc agtggcaggt gccgagatag agcatggcgg
     8941 agtccggata tgggatgcgg gagcgacgtg gatcgttact ccggccgtga cgatccggcg
     9001 ctcgaccagc cgaggcggga ggctcagccg aggccggcga gccgggcgat ctccgcttgc
     9061 aggtcgagga cagcgtcctc ctggtacaga tagcgtatgt gctcgggcag gccggtgcag
     9121 tccacgagcg agatacgccg caggcgatgc atccggacga tcggcagaac gtcggcgagc
     9181 tgcagcccgg aaatgcgggc cagggacagc gactcgagcc gcggcagggt ggcgagcacc
     9241 gggccgacgt tcgtgagcct cgggcagccc cccaggtaga ggtccgtgat cgacgccatg
     9301 ccggcgagcg gcgcgatggt ctcgagcagt ggcgcctccg cgatcgacag cgcgaccagc
     9361 cggcgcaggt gctggatgcc gtcgagcgag cgcaggcgtg gggtgcgcgg cagcgacagc
     9421 tccgccggat cgtcgcacgc cgcgtcgcgc agcgcggcga gggtctcgac cgtgcccgcg
     9481 tcgcgcagtg acaagggccg gggcgtgcgg ggttgtccgg tgccgtgccc gatcgacgcc
     9541 gacggcccgg acgagcggcc cccgctcccc ccgcctcccg cacgtgagcc ctcgcgagcg
     9601 gtgcgcgaga ggctgacgag ccagccgctc gcgacgccga gcgcgccctg tggatcctgg
     9661 gtgacgcccc acaggccgcc gaccagctcc atctcgacgc gctccccgag gacgttccag
     9721 tcgaatggca cgctggtgcg gccgtgcggg aggtcgtcca tgcagttacc ttcgaacggg
     9781 ccgtcgtcga gcgtcgggaa cgtgttgcgc tgcggcggat cgccgacgta cgcgaacagg
     9841 gtgttcagcc agccgtagat gcggtcaccg cccgacgcga tctcgagctt gtatagccgg
     9901 cgccagaact cgcggtcgac gtggccgcgc gcagtggcct cgagctgcgc gagcaccgga
     9961 tcgagcgcct cgacccacca cgccaggtcg aactcctgga acgcggcggc gcgccgccgg
    10021 atcgacgccc agtcctcggg tgtgccggtc agcgtgatct cggggatccc gcacatcgtc
    10081 atgaccgagt acgagaagta ctcccgcatc gcgcccagca gtgcgatctg cgacgcgata
    10141 cgatcgagcg ggcccgtggt cgagaagttg gcgacgaaca gatcggcccg accgccgatg
    10201 tgaccgcgga tctgtgcgac caggttgtcg accacgcccg gccagtcgtt ggccggatcg
    10261 cccgggacga agtcgtcgcg gcggacatcg agcgcgagct tgccggcgtg gcgcacgagc
    10321 cgcgagcgca gggcctcggc gttctgtgcg atgtgcgtgg cgaggctctg cgcgatgcac
    10381 agccagacgg catcgggggt caggacgagc gggcggtggc ccgcgaacgc gaggtgcacg
    10441 gccagcgcga gcgggtggac gtgcgggttg ccgaccagct cggtcgcggc gatgccatgg
    10501 gcctcgggtc tccagcccag cttcgcgagc tgcgatgcgg tggacctcgt cggcagccgc
    10561 gttcgctcgc actccacatc cgacacgcga aacgtcacca tcggccggag catagcgccg
    10621 ctcggcgcac cgttctcggg aatcggcacg cgcatgtggg caagcatctg atacaagagc
    10681 gcgatgacgc tcttcgatct cacaggcaaa gtggcactgg tcaccggttc gaccaagggc
    10741 atcggcaagg cgatcgccga gcgcctcgcg gagcatggcg ccagggtggt cgtgtccagc
    10801 cgcaaggcgg acgcgtgcaa ccaggtcgcc gccggcatca acgcggcgcg cggtgcgcag
    10861 gtcgccgtgc cgatcccgtg caacatcaac tacaaggacc agctgcgggc gctcgtcgac
    10921 gagacccacg agaagctcgg cccgatcgat gtcctggtct gcaacgcggc gttgaacccg
    10981 gcctacggct cgatgtcgca gatcccggac gacgcgttcg acaagatcct cggggccaac
    11041 atccggtcga atcactggct cgcgcagatg gtgctccccg acatgatcgc gaagcgcgac
    11101 ggcgcgatca tcatcgtgtc ctcgatcggc ggctatcgcg gttcgacgac gatcggcacg
    11161 tactgcatct cgaaggccgc ggacctccag ctcgcacgca acctggcggt cgagaacgga
    11221 ccgcacaaca tccgggtcaa ctgcatcgcg ccggggctgg tcaagaccga cttcgcccgg
    11281 gcgctgtggg acacggagga gggggagcgc cgcgcgtcgt ccggcacgcc gctccggcgg
    11341 ctcggcgagc ccgacgacat cgcgggtgcc gccgtgttcc tggccagccg cgccggggcg
    11401 tggatgaccg gacagtcgat catcgtggat ggtggtgcgc tggcctgagg cacgcgcgcg
    11461 cggcggtcac agctcgaagt cgaacaccac gacgcgctcg aaggtcgcgc ggacccgggc
    11521 ggcggcgacc tggtgctggg gatgcgggcc gtaggcggcg aggctcgcgc ggtcggcgaa
    11581 gtccatgctg aagccgtggg tgaagccgtc gcggcgctcg gcgggagcga
//
LOCUS       DASVOR010000390         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266438,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000390 DASVOR010000000
VERSION     DASVOR010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266438"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1612)
                     /locus_tag="VF516_07945"
     CDS             complement(<1..1612)
                     /locus_tag="VF516_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8107646.1"
                     /translation="MSNWFSNFRVSAKFVVLFGGVLLASMALGVYFIINLDRVSRQAS
                     SLAATRVGSAKHLGGVTTHIARFRVQQLLHIVAPDDAEKANLTPTLQDEKDAVEKDIA
                     AYKELVAAGSERATLDRVEADLREFFRVHEQVTVLSRNHKEADAIALMKGEALRVRNQ
                     LVAGLAELNKANDQATEDAKADAARSYRDARLASIIAMLALLVLVIAAARFGTRQIVS
                     SINLVSGQLQELASGEADLAKRLDATAGDELGELARNFNKLMAQLEAIVRQAKGSGLQ
                     VTRASAQLAEGSKQLEATVSQQVASTHDVVSTTEQISTTAQSLLDTMNQVSQTAQSTA
                     KSAALGQARLEETRNSMNVLKEAADAISSRLAAINEQAGNISSVVTTITKVADQTNLL
                     SLNAAIEAEKAGEFGQGFSVVAREIRRLADQTAVATLDIEKMVKDMRSAVSSGVASVD
                     KFSSEVRDVALVVRDTVAQFVAIVEQVRALTPRFDAVAEGMSDQSVNAKQISVAMVQL
                     RATSERTQAAMRDSAQSIEALNESARALE"
     gene            complement(2158..2418)
                     /locus_tag="VF516_07950"
     CDS             complement(2158..2418)
                     /locus_tag="VF516_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107647.1"
                     /translation="MPWIARRTTSMLAWQSEPELNVLCNPVGALKLRGVEIATWQAID
                     LPRKWGDTSRKPDKGPHDQLAAMFKRLKAPLNACPTATAPSP"
BASE COUNT          477 a         1019 c         1081 g          521 t
ORIGIN      
        1 gctccagcgc ccgcgcgctc tcgttgaggg cctcgatcga ctgcgccgag tcgcgcatcg
       61 ccgcctgcgt gcgctcgctg gtcgcgcgca gctgcaccat cgcgacggag atctgcttgg
      121 cgttgaccga ctggtccgac atgccctcgg cgaccgcgtc gaaccgcggg gtcaacgcgc
      181 gcacctgctc gacgatggcg acgaactggg cgacggtgtc gcgcacaacg agcgcgacgt
      241 cgcggacctc gctcgagaac ttgtcgacgc tcgcgacgcc cgacgacacg gcgctgcgca
      301 tgtccttgac catcttctcg atgtcgagcg tggcgaccgc ggtctggtcg gcgagccggc
      361 ggatctcccg cgcgacgacc gagaagccct ggccgaactc gcccgccttc tcggcctcga
      421 tcgcggcgtt gagcgacagc aggttggtct gatcggcgac cttggtgatc gtcgtgacga
      481 cgctcgagat atttccggcc tgctcgttga tcgcggcgag ccgcgacgag atcgcgtcgg
      541 cagcctcctt gagcacgttc atcgagttac gggtctcctc gagccgcgcc tggccgagcg
      601 ccgccgactt tgcggtcgac tgcgccgtct gcgagacctg gttcatcgtg tcgagcaacg
      661 actgcgccgt ggtggagatc tgctcggtgg tcgacacgac gtcgtgggtc gaagccacct
      721 gctgcgacac ggtggcctcg agctgcttgg agccctcggc gagttgagcc gacgcgcgcg
      781 tgacctggag gccggagccc ttggcctggc gcacgatcgc ctcgagctgc gccatcagct
      841 tgttgaagtt gcgcgccagc tcgccgagct cgtcgccggc cgtggcgtcg aggcgcttgg
      901 cgaggtcggc ctcgcccgag gcgagctcct ggagctgacc gctgaccagg ttgatcgagc
      961 tgacgatctg gcgggtgccg aaccgcgccg cggcgatcac gagcacgagc agggcgagca
     1021 tcgcgatgat cgacgccagc cgcgcatcgc ggtacgaccg cgccgcgtcg gccttggcgt
     1081 cctcggtggc ctggtcgttg gccttgttga gctcggcgag cccggcgacc agctggttgc
     1141 ggacccggag cgcctcgccc ttcatgagcg cgatcgcgtc ggcctccttg tgattgcgcg
     1201 agagcacggt gacctgctcg tggacgcgga agaactcgcg gagatcggcc tcgacccggt
     1261 cgagcgttgc gcgctcggaa ccggcggcga ccagttcctt gtacgcggcg atgtccttct
     1321 cgaccgcgtc cttctcgtcc tggagcgtgg gcgtgaggtt ggccttctcg gcgtcgtcgg
     1381 gcgcgacgat gtgcaggagc tgctgcacgc ggaaccgcgc gatgtgcgtg gtcacgccgc
     1441 ccagatgctt ggcgctgccg acacgggtcg cggcgagcga gctcgcctgc cgcgataccc
     1501 gatcgaggtt gatgatgaag tacacgccca gggccatgct ggcgagcagc acaccaccga
     1561 acaggacgac gaactttgca gagacccgga agttggagaa ccagttggac atggcgcagg
     1621 ctcgagccgc gtcggtgcta tcataggagc aagccgaggc ggcactcaag cgtctggcct
     1681 gcggacgagc gagtccctgg tgatccggac cgtcattgca gcggtatgtg cggccgccca
     1741 caaaggcgga cggtcgccgt gcacgcggga tcggtgaacc cgatgcggcc ctcctcgctc
     1801 gtgaagtggc gacggcacca catcggccgg ccgccgagcc ggtgttcgcg gaaccgactc
     1861 caggtccatt gccgggacgg cgccgcggag cgtgtgaccg cgcgacgcgc gccgggcgcg
     1921 caggctcgac gcgctccgcg aggtagagat ccacacaggt tccgaacagg gtcagcttgc
     1981 ttgccgggcg gcttgagctt ctgcgcgccg tcgatgggtg gtcgacagcc cgtccgccag
     2041 gttcagctca ccaccaccac cgactctccc ggcttacccg ctgcagccca gagtgacgcg
     2101 gcccgagctg gccgcggtca cggcaatcca ccgcctgctg ccggccgcgt tcggtggtta
     2161 ggggcttgga gcagtcgcgg tcgggcatgc gttgagcggg gccttgaggc gcttgaacat
     2221 cgccgcgagc tggtcgtgtg gacccttgtc cggcttgcgg ctcgtgtcgc cccacttgcg
     2281 tggcagatcg atcgcctgcc acgtcgcaat ctcgacaccg cgcagcttca gcgcgccgac
     2341 tgggttgcac agtacgttca gctccggctc gctttgccag gccaacatcg aggttgtcct
     2401 ccgggcgatc cacggcatcg tcggcgacgt cgagctcggt gtcggtggca ggctgcgccg
     2461 ggcgagtgag ctcgtgcacg cgggtcatga acgagccggc ggagtcgaac tggacctcgt
     2521 cgctggtgcc gtcgaacagg ccgacgaaca gagcgcgctt gtcgcccacc aagccggcga
     2581 tcgctcatcg aaaggacttc gcgaagtgct cccctatcga aattagcatg ggaaacatgg
     2641 gcaacccctt gaacgcgctc gagaaatcgc cactgcggaa aaggaatgag cgcgtaaaaa
     2701 gttgagatca ttcgctcttg gctggattta ggttcctgtc cgtaacaggg tccgggttcg
     2761 agtcccggcg ctcgcacgcg ccttttcgag gacatggaaa gccctcaaag gcttcttgaa
     2821 agtcacgaaa ggctttcgcg aagtcagtat cgccggcggt cacccggtgc agcgaagtgg
     2881 ccgcgcgcgg tcgaccgcag tggcagagtt gccgcgtgaa cgaccccgat gctgccgatc
     2941 ggaccgccag acgaggacag aagcaacacg ggcatgagcc tggggaaagc cgcgtgtgtc
     3001 ggtcgatttg tgagcgtccc gcggagtcgg cctaacgcgc cgcggccacg cttgtgcgcg
     3061 gcggccagcc gtggcaggcg cgcggggctc aggtctgc
//
LOCUS       DASVOR010000391         2464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_325049,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000391 DASVOR010000000
VERSION     DASVOR010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325049"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1513
                     /locus_tag="VF516_07955"
     CDS             167..1513
                     /locus_tag="VF516_07955"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8107648.1"
                     /translation="MTTPSMFSRPRLAACTAALIALAACGGGGAGTSARGSTVPPPPK
                     ITRSEAAAARAEPRREVSKDTRSDFDSAVQQFTATDKAHGWNDSTCRQSADRFQAVAR
                     AHPDLIEAQAMVGVSYQRCGMLDDAERVYRQVIQVKPNHGASLSNLGEIYFRTGRASE
                     ARQYWDSAIKANGKLVGARIGVATLELEQMRKIGNPKDATWKKLEEDARFNLSSALGV
                     DSDSAAAYTVYGLVYMEGWQTNKNRLDLAKLLLDEASKRNEKYAPLQNAYGLYYMRRA
                     SINQALQAFNAAVELDPRFVEARMNVGQVTLGFRKYDTARDMFSKVIELSPKNYDAYI
                     GLGIALRGLKDLDGAEAQYKKAKDLDPRRGEAYYNLAILYKEFRSSKEDFIASYKQAK
                     EYFQQFLTMQADQADKNEAKEQIAMIDKTVQNFMKAPPPTPAAAPQAAPAQPPAKP"
     gene            1682..1975
                     /locus_tag="VF516_07960"
     CDS             1682..1975
                     /locus_tag="VF516_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107649.1"
                     /translation="MQKILGVFVVTLVVITASVAVTDPAHAQPAAKTSDKGNDKAAGS
                     KVKVYDFSGDTIEGDLIRPEGTTVDARDFAKHSSLIRIRTNFIPEIIKSAEDL"
     gene            2065..>2464
                     /locus_tag="VF516_07965"
     CDS             2065..>2464
                     /locus_tag="VF516_07965"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8107650.1"
                     /translation="MAGAKVPLTFRIFKGDQLLREERLSLSVIKVGKVPSAHLKLDDE
                     TVSRMHAIIEVNGPGDVSIIDLGSTKGTFVNGQKVNKAKLQSGDTINVGETRIELAIG
                     AEDEDAPTRVSVAHAPPPSAAEVIASTPRPT"
BASE COUNT          483 a          875 c          786 g          320 t
ORIGIN      
        1 aagctgtgcg agcgcgagct cggccagatc cggcccgagg agtacccgac cacctcggag
       61 ctgcgcagcg atcccaacca ggtcgcgccg atcaccgata tcgatgtgcc gacgaagctg
      121 gactgaccgc gtgccctcga cccaccccag gacggatgcg atgaccatga cgacgccatc
      181 gatgttctcg aggccacgcc tcgcagcctg taccgccgcg ctgatcgcac tcgccgcgtg
      241 cggcggcggc ggcgccggta ccagcgcacg gggcagcacc gtgccgccgc cgccgaagat
      301 caccaggtcc gaggccgccg ccgcccgggc ggagccgcgg cgcgaggtgt ccaaggacac
      361 ccgcagcgac ttcgacagcg cggtgcagca gttcaccgcg accgacaagg cgcacggctg
      421 gaacgactcg acgtgccggc agtcggccga ccggttccag gccgtggccc gcgcccaccc
      481 cgacctgatc gaggcccagg ccatggtcgg cgtgtcgtac cagcgctgcg ggatgctcga
      541 cgacgccgag cgcgtgtacc gccaggtgat ccaggtcaag cccaaccacg gcgcgtcgct
      601 gtccaacctc ggcgagatct acttccggac cgggcgggcc agcgaggccc gccagtactg
      661 ggacagcgcg atcaaggcca acggcaagct ggtcggggcg cggatcggcg tcgcgacgct
      721 cgagctcgag cagatgcgca agatcggcaa ccccaaggac gccacctgga agaagctcga
      781 ggaggacgcc cggttcaacc tgtccagcgc gctcggcgtc gacagcgaca gcgccgccgc
      841 ctacacggtc tacggcctgg tctacatgga gggctggcag accaacaaga accggctcga
      901 tctcgccaag ctgctgctcg acgaggccag caagcgcaac gagaagtacg cgccgctgca
      961 gaacgcctac ggcctgtatt acatgcgcag ggcatcgatc aaccaggcgc tgcaggcgtt
     1021 caacgccgcg gtcgagctcg acccgcggtt cgtcgaggcg cggatgaacg tcggccaggt
     1081 caccctgggg ttccgcaagt acgacaccgc gcgcgacatg ttcagcaagg tgatcgagct
     1141 gtcgcccaag aactacgacg cctacatcgg cctgggcatc gcgctgcgcg ggctcaagga
     1201 cctcgatggc gccgaggcgc agtacaagaa ggcaaaggac ctcgatccca ggcgcggcga
     1261 ggcctactac aacctcgcga tcctctacaa ggagttccgg tccagcaagg aggacttcat
     1321 cgcgtcctac aagcaggcca aggagtactt ccagcagttc ctgaccatgc aggccgacca
     1381 ggccgacaag aacgaggcca aggaacagat cgcgatgatc gacaagaccg tgcagaactt
     1441 catgaaggcg ccgccgccca cgccggccgc ggcgccccag gcggccccgg cccagccgcc
     1501 cgcaaagccg taaccaccga gaattccttg gccaagaacc cgcaaccccg acccggatcc
     1561 gtgtgtctcg atgtacgcgg agtttcgggc acaatctggg aacctcgaac tcggacgcgg
     1621 tgtcggagga tccgtgccac cggatccctg ccacccggcg tcctcgcaag ggagcaccgc
     1681 catgcagaag atcctgggtg tcttcgtcgt cacgctcgtg gttatcaccg cctcggtggc
     1741 cgtcaccgac ccggcgcacg cgcaacccgc cgcgaagacc agtgacaagg gcaacgacaa
     1801 ggccgccggc tcgaaggtga aggtctacga cttctccgga gacaccatcg aaggcgacct
     1861 gatccggccg gagggcacca cggtcgacgc ccgcgacttc gccaagcact cgtccctgat
     1921 ccgcatccgc acgaacttca tccccgagat catcaagtcg gccgaggacc tgtagcctct
     1981 cgtcgggcga tcggggcgcg tccgcgctcg cgatcatcgt cggccgttcg cgacccaagc
     2041 gctcctttcc tggagaactg aattatggcc ggcgccaagg ttcctctcac gtttcgcatc
     2101 ttcaagggtg accagctgct ccgcgaggag cggctgtcgc tcagcgtcat caaggtcggc
     2161 aaggtcccct cggcgcacct caagctcgac gacgagacgg tgagccgcat gcacgcgatc
     2221 atcgaggtca acggccccgg tgacgtcagc atcatcgacc tgggctcgac caagggcacg
     2281 ttcgtcaacg gccagaaggt caacaaggcc aagctgcagt cgggcgacac gatcaacgtc
     2341 ggcgagaccc ggatcgagct ggcgatcggc gccgaggacg aggacgcgcc gaccagggtc
     2401 agcgtggccc acgcgccgcc gccgagcgcc gccgaggtga tcgcgtcgac cccgcggccg
     2461 accc
//
LOCUS       DASVOR010000392         2286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_237222,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000392 DASVOR010000000
VERSION     DASVOR010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237222"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VF516_07970"
     CDS             complement(<1..1054)
                     /locus_tag="VF516_07970"
                     /inference="COORDINATES: protein motif:HMM:NF025803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger family 4 domain-containing protein"
                     /protein_id="HEX8107651.1"
                     /translation="MDSIRTLLLARTHTVVLDPDRVASAATRPSRGVDLEKFEDELAQ
                     LGHVMSLDLAMTVRRLPHQALLELRAWILSTLTRSLGAHRAHVPLAGGAASATGDGPS
                     LHLRRMLTWLATRSAQPCPWCGQVKPIGALDPCGHLVCRSCWDGGTFAGCPICHRRVA
                     VGEPFVRASEIADRVTHHDGQLRLLHLGFDLIGAARSRFEQLIGRTAPLSLDDRAEVE
                     TVIDAMGPRTVQWLPIRIPVKETMAVVLARLWTVAPDRIAMMHATRAHLSTATDVLRV
                     AVVLLGGNPALTEPMRLGSLGRSMRRAVLDALDHLPADPLIEDMWRHRGLWKRVGERL
                     HPYEYATRLPVAALGFA"
     gene            complement(1747..>2286)
                     /locus_tag="VF516_07975"
     CDS             complement(1747..>2286)
                     /locus_tag="VF516_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8107652.1"
                     /translation="RAMYKALRDATITAALRERFRIVHISLQRTHVHLLVEADHKTAL
                     ARGMQGFTVSAARHINTALGSGARRRRGKVFADRYHVEVITTPTRARHALGYVLGNWR
                     KHGEDRGNLASTWLVDPFSSAISFPDWQELQDQPWMWPIRETYDPLVVRRPQTWLLRE
                     GWKVGGGSISVRDVPSKPR"
BASE COUNT          338 a          836 c          801 g          311 t
ORIGIN      
        1 ccgcgaaccc gagcgccgcg accggcagcc gggtcgcgta ctcgtaggga tgcagccgct
       61 cgccgacccg cttccacagc ccgcggtggc gccacatgtc ctcgatcagc gggtcggccg
      121 gcaggtggtc gagcgcgtcg agcaccgcgc gccgcatgct gcggccgagc gagcccagcc
      181 gcatcggctc ggtcagcgcc gggttgccgc cgagcagcac caccgcgacg cgcagcacgt
      241 cggtcgcggt cgacaggtgc gcgcgcgtcg cgtgcatcat cgcgatgcga tcgggcgcga
      301 ccgtccacag ccgggcgagc accacggcca tcgtctcctt gaccgggatc cggatcggca
      361 gccactgcac ggtcctgggg cccatcgcgt cgatcacggt ctcgacctcg gcgcggtcgt
      421 cgagcgacag cggcgcggtc cggccgatca gctgctcgaa ccggctgcgc gcggcgccga
      481 tcagatcgaa ccccagatgg agcagccgga gctggccgtc gtggtgcgtg acccggtcgg
      541 cgatctcgga cgcgcggacg aacggctcgc cgaccgcgac ccggcggtgg cagatcgggc
      601 agccggcgaa cgtgccgccg tcccagcacg accggcacac caggtggccg cacgggtcca
      661 gcgcgccgat cggcttgacc tggccgcacc acgggcacgg ctgggccgac cgggtcgcga
      721 gccaggtcag catccggcgc aggtgcagcg agggcccatc gccggtcgcg ctcgccgcgc
      781 cgccggccag cgggacgtgc gcgcggtggg cgccgagcga gcgggtcagc gtcgagagga
      841 tccacgcgcg gagctcgagc agcgcctggt gcggcagccg ccgcaccgtc atcgcgaggt
      901 cgaggctcat cacgtggccg agctgcgcga gctcgtcctc gaacttctcc agatcgacgc
      961 cgcggctcgg ccgggtcgca gcgctggcga cgcgatcggg atcgagtacg accgtgtgag
     1021 tgcgcgccag cagcagcgta cggatcgagt ccatcgagag atgggcggag agtggctgcg
     1081 ctaccgtgac caaccaggaa gcacagcccg agccgcccgc ctgcactcta gcaccgttac
     1141 cggcgccgtt gtcgggtggc gggtgcccgg ccgaaacacc gggcggggtc gcaccaggat
     1201 ccggatccga tcatgcgtcg ccatgtcgca ccggcggcag gcatccttcc gctcagcggc
     1261 agcaccgcgg tgcggttgtg ccggccgccc ggcagtgtga ccggcacggt gcgctcgatc
     1321 gccaaggccg cgaccaacga catccacgtc gcggtctggg gccagacccg gccggcgatc
     1381 cggcgaccgg cggcgggcat ctacctgacc tgcgatcagg tcggtcccgc gggcggcacg
     1441 tacttcgagc acgacgccgg catcgccgtg caggacctgc cgctggtctg gtcgctcacc
     1501 gtcgacccat cgagcgtgac gccgacctcg cgcctgtgcg ggacggcgac attcggccgg
     1561 cgccgaggtc gtcaccagcg ccggcctcta cgcctcgacc gacgctggtg cgcactgggc
     1621 gctcgccggc ctcgagggca agatcgtccg aggcgtcacg ctggagcccg accacgcggt
     1681 cggcgtcgcg cctcggatcg aacggctact cgctgctgcg ggtcgagctc gcttgaacag
     1741 gaggcgtcag cgcggcttgc tcggcacgtc gcgcacgctg atcgagcccc caccgacctt
     1801 ccagccctct cgcagcaacc atgtctgggg tctccgcacc accagcggat cgtaggtctc
     1861 gcggatgggc cacatccatg gctggtcctg cagttcctgc cagtcgggga atgagatggc
     1921 gctcgagaaa ggatccacga gccacgtgct cgccaaattg ccgcggtctt ctccgtgctt
     1981 tctccaattc cccaacacgt accccagagc gtgacgcgcc cgcgtcgggg tcgtgatgac
     2041 ctcgacgtgg tagcgatcgg caaacacctt gccacggcgc cggcgcgcgc cgctccccag
     2101 cgcagtgttg atgtgcctcg ccgccgaaac cgtaaagccc tgcatcccgc gggcaagcgc
     2161 tgtcttgtga tcggcctcca cgagcaagtg aacgtgcgtg cgctgcaacg agatgtgtac
     2221 gatccggaac cgctcgcgca gcgccgccgt gatcgtggca tcgcgcagcg ccttgtacat
     2281 cgcccg
//
LOCUS       DASVOR010000393         2093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_529651,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000393 DASVOR010000000
VERSION     DASVOR010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2093
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529651"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF516_07980"
     CDS             <1..180
                     /locus_tag="VF516_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107653.1"
                     /translation="AAAGRTGDVAERAHLAYAVQQIAELERDPHRIEAPPAEPPDGPP
                     IGAPDANTDTDDDEP"
     gene            complement(314..1987)
                     /locus_tag="VF516_07985"
     CDS             complement(314..1987)
                     /locus_tag="VF516_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HEX8107654.1"
                     /translation="MINDLWYKNAVLYCLDVGTFMDANGDGVGDFGGLMRRLDYLAGL
                     GINCVWLMPFQKSPRRDDGYDITDYYQVDERFGTLGDFVDFTHEAKQRGIRVMIDLVV
                     NHTSDQHPWFQAARRDKHSKYRDYYLWADRRPRDADHGMVFPGVQKTTWTRDPVAGKY
                     YFHRFYDFQPDLNTQNPEVQEEIRKIMGFYLQLGVCGFRMDAMPFVIQSKGAGKRIVE
                     HYAMLREFREFLQWRSGDAIILAEANVVPDMSINYFGDDGDRLHMMFNFWVNQRVFYG
                     LATADSRPLKKALEQTRSRPPTGQWAHFLRNHDELDLGRLDDAQRQRVFDAMGPARTM
                     QLYDRGIRRRLAPMLDGDRRRIEVAYSLLFTLPGTPVLRYGDELGMGDNLRLKERNCA
                     RTPMQWSTEPHAGFTRSDRPILPVIEHGPYGYHKVNAAEQRRDPESLLNWTERIIRMR
                     KEVPEIGWGKFSLVDTGHDSAIAIRYDWRNNSVVTMHNLGSEAIEVQLRLGDARARTL
                     VNLLTEDHSDADRRGRHTVVLEPYGYRWYRVGGLGYLLDRGVPGREATR"
BASE COUNT          311 a          731 c          715 g          336 t
ORIGIN      
        1 gccgcggccg ggcgcaccgg cgacgtcgcc gagcgcgccc acctcgccta cgccgtgcag
       61 cagatcgccg agctcgagcg cgatccgcat cgcatcgagg cgccgcccgc cgagccgccg
      121 gacggcccgc cgatcggcgc cccggacgcc aacaccgaca cggacgacga cgagccgtag
      181 caccgcgctc agctcagcga tgggggtcgc cggcgcgtgg gccacggcgt cggcgatcta
      241 cggcttcacc gagaacgcgc ggtgtcgctc ggcgctcgcc gggccggcgt ccgagccggc
      301 gcgatgatcg ggactaccgg gtcgcctcgc gccccggcac cccgcgatcg agcaggtatc
      361 ccaggccgcc gacgcggtac cagcgatagc catagggctc cagcaccacg gtgtgccggc
      421 cgcggcgatc cgcgtcgctg tgatcctcgg tcagcaagtt gaccagcgtg cgcgcccgtg
      481 cgtcgcccag ccggagctgg acctcgatcg cctcgctgcc gaggttgtgc atggtcacga
      541 cgctgttgtt gcgccagtcg tagcggatcg cgatcgcgct gtcatggccg gtatcgacca
      601 gcgagaactt gccccagccg atctccggaa cctccttgcg catgcggatg atccgctcgg
      661 tccagttgag cagcgactcg ggatcgcggc gctgctcggc cgcgttgacc ttgtgatagc
      721 cgtacggccc gtgctcgatc accggcaaga tcgggcgatc gctgcgggtg aacccggcgt
      781 gcggctcggt cgaccactgc atcggcgtgc gcgcgcagtt gcgctccttg agccgcaggt
      841 tgtcgcccat gccgagctcg tcgccgtagc gaagcaccgg cgtgccgggc agcgtgaaca
      901 agaggctgta ggcgacctcg atccgccggc gatcgccgtc gagcatcggc gcgagccgcc
      961 gccggatgcc gcggtcgtag agctgcatgg tcctggccgg ccccatcgcg tcgaacaccc
     1021 gctggcgctg cgcgtcgtcg agccgaccga gatcgagctc gtcgtggttg cgcaggaagt
     1081 gcgcccactg cccggtcggc ggccggctgc gggtctgctc cagcgccttc ttgagcggcc
     1141 ggctgtcggc ggtggccagc ccgtagaaca cccgctggtt gacccagaag ttgaacatca
     1201 tgtgcagccg gtcgccgtcg tccccgaagt agttgatcga catatcgggg acgacgttgg
     1261 cctcggcgag gatgatcgcg tcgccgctcc tccactgcag gaactcgcgg aactcgcgca
     1321 gcatcgcgta gtgctcgacg atgcgcttgc cggcgccctt gctctggatc acgaacggca
     1381 tcgcgtccat gcggaacccg cacacgccga gctggagata gaaccccatg atcttccgga
     1441 tctcctcctg gacctcgggg ttctgcgtgt tgagatcggg ctggaagtcg tagaaccggt
     1501 ggaagtaata cttgccggcg acgggatcgc gcgtccacgt cgtcttctgc acgccgggga
     1561 acaccatccc gtggtcggca tccctgggcc gcctgtccgc ccacagatag taatcgcgat
     1621 atttcgagtg cttgtcgcgc cgcgccgcct ggaaccacgg gtgctgatcc gaggtgtggt
     1681 tgaccaccag atcgatcatc acgcggatcc cgcgctgctt ggcctcgtgg gtgaaatcga
     1741 cgaagtcgcc gagcgtgccg aaccgctcgt cgacctggta gtagtcggtg atgtcgtagc
     1801 cgtcgtcgcg ccgcggcgac ttctggaacg gcatcaacca cacgcagttg atgccgagcc
     1861 cggcgaggta gtcgagccgg cgcatcaggc ctccgaagtc gccgaccccg tcgccgttgg
     1921 cgtccatgaa cgtgccgacg tcgaggcagt agaggaccgc gttcttgtac cagaggtcgt
     1981 tgatcacggg ccggtggccc tcctcgcgtc agggcggcgc gaccagcgcc gccggcgtga
     2041 tcggcagctc gcggatccgc cggccggtcg ccgcgaacac cgcgttggcg acc
//
LOCUS       DASVOR010000394         4247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_471326,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000394 DASVOR010000000
VERSION     DASVOR010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471326"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VF516_07990"
     CDS             <1..571
                     /locus_tag="VF516_07990"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8107655.1"
                     /translation="GDLRPPPLLDVQALRAQAASRPDVRVREAERAVAAAENDQAQAN
                     ALPQVSVWAGYLREDTDAIVLGGFRITLPLWNRAQGDRETAAAKERRAIALRDATVRA
                     AERQIADAIAAYTLARDAVDAFERDVVPVLDDSEQLLQKTIDAGQIAVSDYLVARQEI
                     LDGRREYLERLLALARAAATARFVAGGAP"
     gene            568..1746
                     /locus_tag="VF516_07995"
     CDS             568..1746
                     /locus_tag="VF516_07995"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8107656.1"
                     /translation="MTRAVVIALVLCACGDRKASDPDHRPAESVEHAGPDVANGGKPH
                     AHGPDMIKIAPDMMRDLRVTTGKAQARAAVETVSALGDLRVNEDAYAEVASPVSARVT
                     RVLVRPGDAVKAGQALVELNSPELVQERAELEAARSRLEIARKNAERKRALAADRLVP
                     EREVIEAESALTEADAAYKVAASALRKFGTAAGEAVLAIRSPIAGTVIERSVVQGQLA
                     DPSKTLFRIGDLSTLWLHAHVFERDAVRVQTGRTATATFAALPGKSTEAIIQWIGREV
                     DASSRTIDIRLDVPNPDGVLRPGMSATVSIPLGDTGPNEVVTIPAAAVQRVGASWAVF
                     IPHGPGQFAIRPIGRGRDLAGEVEVLSGLVAGNEVVVDGAFLLKAEADKASGGAEHED
                     "
     gene            1755..>4247
                     /locus_tag="VF516_08000"
     CDS             1755..>4247
                     /locus_tag="VF516_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8107657.1"
                     /translation="MLERLITASFRKPLIAIVLAIAGAAIGTVWMRALPRDVFPDLSA
                     PVFNIIVQNPAMNADELEMRVAIPFETALSGLPQVRRVRSSSTLGVVQITIEFEPDAD
                     YARSRQYVAERLPQVELPPGTETPLLSGVTGRLNEIFEITVEARPGVADLMTLRDLAE
                     NEIKNRLLAVPGVSAVERLGGYLREFQIQIDPDRMAARGVTLDEIMHAARGANASAAG
                     GFVTQAQMDWTVRAAGRAANVEQLRNTVVVMKGGIPVLLGDVADVREAPAVRRGIAHR
                     LAGEVVSCRIVKQFGSDTVAVARGIRGAIADLRGSLPRGVEIRIVYDQSELVESSLGG
                     VGRAVLIGAALVALVLFALLGNVRAALIVTLTLPLSLALSGVFMRVAGIGINTMTLGG
                     LAIAVGLLVDSSIIVVENVVHRISQRRDGDRRAHALAAAAEVGRPIAFATAVVVAVFV
                     PLFAMTGIEGRMYKPLAGAVIAAVLAALVLSLALVPVVAAGLLRPTRAGAHEDVWLVR
                     KLKQAYRPVLDRALRHTAIVTIVALVITAPALWIATRLGSDFVPPLDEGAILMQTFLP
                     PEASLEEVDRLNHRVEDVLRTFPEVSDVVRRTGRAERTEDPMPHTLSDVLILLRPERA
                     RTGEALVKEMRDAVEKVPGVSTLFTTPLGMRIDEGLGGTPADISVRIFGPDAQQLADL
                     AESAAATIRAIDGLEDVRAEPLVGLPQLTVQVDREAAARVGLTPGDVVEAVQIGLAGQ
                     EVSEIWIGQRRFGLTVKLSPDFRRDAATLAALRIDGHDGSRIPLGKLARIEPAFGLGT
                     IKREAGSRRIAVEASVSGRDLGSAARDVRDALA"
BASE COUNT          588 a         1486 c         1570 g          603 t
ORIGIN      
        1 tggcgatctc cggccgccgc cgttgctcga tgtgcaggca ctgcgcgcgc aggcggcgag
       61 ccggccggat gtccgcgtgc gggaagccga gcgcgcggtc gccgcggcgg agaacgacca
      121 ggcgcaggca aacgcgctgc cgcaggtctc ggtgtgggcg ggctacctgc gcgaggacac
      181 cgacgcgatc gtgctcggcg ggtttcgcat cacgctgccc ctctggaacc gcgcacaagg
      241 cgaccgagag acggcggcgg cgaaggagcg gcgcgccatc gcccttcgcg atgcgacggt
      301 ccgtgctgcc gagcgccaga tcgcggacgc gattgcggcc tacacgctgg ccagggacgc
      361 ggtcgacgcg ttcgagcgcg acgtcgtgcc tgtgctggac gacagcgagc agctcctgca
      421 gaagacgatc gacgccgggc agatcgcggt gagcgactac ctcgtcgcgc ggcaggagat
      481 cctcgacggc cggcgcgagt acctcgagcg gctgctcgcg ctcgccaggg ccgccgccac
      541 ggcgcggttc gtggctggag gtgcgccatg acccgggctg tcgtcatcgc gctcgtgctg
      601 tgcgcctgcg gcgacaggaa ggccagcgac cccgatcacc ggccggccga gagcgtcgag
      661 catgccggcc cggacgtggc gaacggcggg aagccgcacg cgcacggtcc agacatgatc
      721 aagatcgcgc ccgacatgat gcgcgacctg cgcgtcacca cgggaaaagc acaggcgcgc
      781 gccgccgtcg agaccgtctc cgcgcttgga gacctgcgcg tcaatgaaga cgcgtacgcc
      841 gaggtggcat cgccggtcag cgcacgcgtg acccgcgtgc tcgtccggcc tggtgacgcg
      901 gtgaaagccg ggcaagcgct ggtcgagctc aatagccccg agctggtgca agagcgcgcc
      961 gagctcgaag ccgcgcgatc ccggctcgag atcgcgcgca agaacgccga gcgcaagcgc
     1021 gcgctcgcgg ccgatcgcct ggttcccgag cgcgaggtga tcgaggccga gtccgcactc
     1081 accgaggccg acgcagcgta caaggtcgcc gcctcggcgc tgcgcaagtt tggcaccgcg
     1141 gccggcgagg ccgtgctcgc gatccggtcc ccgatcgccg ggacggtgat cgagcgcagc
     1201 gtggtgcaag gtcagctcgc cgacccatcg aagaccctgt tccggatcgg cgatctgtcc
     1261 acgctgtggc tccacgcgca cgtgttcgag cgcgacgcgg tgcgggtgca gaccgggcgc
     1321 accgcgaccg caacgttcgc ggcgctcccc ggcaagtcga ccgaggcgat catccagtgg
     1381 atcggccggg aggtggacgc ctcgtcgcgc acgatcgaca tccggctcga cgtcccgaac
     1441 cccgacggtg tgctgcgccc gggcatgtcc gccacggtct cgatcccgct gggcgacacc
     1501 gggccgaacg aggtcgtcac cattccggct gccgccgtgc agcgggtcgg cgcgagctgg
     1561 gcggtgttca tcccgcacgg tccgggacag ttcgcgattc gcccgatcgg tcgcggtcgc
     1621 gacctggccg gcgaggtcga ggtgctctcc gggctggtcg ccggcaacga ggtcgtggtc
     1681 gatggtgcgt tcctgctcaa ggccgaggcc gacaaggcga gcggcggcgc agagcacgag
     1741 gactaggagg cggcatgctg gagagactga tcaccgcgag cttccgcaag ccgctcatcg
     1801 cgatcgtgct ggcgatcgcc ggcgccgcca tcggcaccgt gtggatgcgc gcgctgccgc
     1861 gcgacgtgtt tcccgatctg tccgcgcccg tgttcaacat catcgtccag aacccggcga
     1921 tgaacgccga cgagctcgag atgcgggtcg cgattccgtt cgagaccgcg ctgtcgggcc
     1981 tgccccaggt gcgccgcgtg cgctcgtcgt cgacgctggg cgtcgtgcag atcacgatcg
     2041 agttcgagcc ggacgccgat tacgcgcgct cgcggcagta cgtcgccgag cgcctgccgc
     2101 aggtcgagct ccccccgggg accgagacgc cgctgctctc gggcgtgacc ggccggctga
     2161 acgagatctt cgagatcacc gtcgaggcca ggcccggcgt cgcggacctc atgacgctgc
     2221 gcgacctggc cgagaacgag atcaagaacc gcctgctcgc ggtgcccggt gtgtcggccg
     2281 tcgagcggct cggcggctac ctgcgcgagt tccagatcca gatcgatccg gatcgcatgg
     2341 cggcgcgcgg ggtcaccctc gacgagatca tgcacgccgc gcgcggcgcc aacgcgagcg
     2401 ccgcgggcgg cttcgtgacc caggcgcaga tggactggac cgtgcgggcg gccggtcgcg
     2461 cggcgaacgt cgagcagctg cgcaacaccg tcgtcgtgat gaaaggtgga atccccgtgc
     2521 tcctgggcga cgtcgccgac gtccgcgagg cgcccgcggt gcggcgcggc atcgcgcacc
     2581 gcctcgccgg agaggtcgtg agctgccgga tcgtgaagca gttcggctcc gataccgtgg
     2641 ccgttgcccg gggcatccgc ggcgccatcg ccgatcttcg cggcagcttg cccaggggcg
     2701 tcgagatccg gatcgtctac gaccagtccg agctggtcga gtcctcgctc ggtggcgtcg
     2761 ggcgcgccgt gctgatcggc gcggcgctgg tggcgctcgt gctgtttgcc ttgctcggca
     2821 acgtccgcgc cgcgctgatc gtgaccctga cgcttccgct gtcgctcgcg ctctcgggcg
     2881 tcttcatgcg ggtcgccggc atcggcatca acacgatgac gctcggcggc ctcgcgatcg
     2941 ccgtgggcct cctggtcgac tcgtcgatca tcgtggtgga gaacgtcgtg catcggatct
     3001 cgcagcgtcg cgacggcgac cggcgcgccc atgcgctcgc ggcggccgcg gaggtggggc
     3061 ggccgatcgc gttcgcgacc gcggtggtgg tcgccgtgtt cgtcccgctg ttcgcgatga
     3121 ccgggatcga agggcgcatg tacaagccgc tcgcgggcgc cgtgatcgcg gccgtgctcg
     3181 ccgcgctcgt gctctcgctg gcgctcgtgc cggtggtcgc cgccggcctg ttgcgcccga
     3241 cccgcgcggg cgcccacgag gacgtgtggc tggtgcgcaa gctcaagcag gcgtatcgcc
     3301 cggtgctcga ccgagcgctc cgtcacacgg cgatcgtgac gatcgttgcc ctcgtcatca
     3361 ccgcgccggc gctctggatc gcgacgcggc tcggctcgga cttcgtgccg ccgctcgacg
     3421 agggcgcgat cctcatgcag acgttcctcc cgccggaggc gtcgctcgag gaggtcgatc
     3481 gcctgaacca tcgcgtggag gacgtcctgc gcacgttccc ggaggtctcc gacgtcgtgc
     3541 ggcgaaccgg gcgtgccgag cgcaccgaag atccgatgcc gcacacgctc tcggacgtgc
     3601 tgatcctgct caggccggag cgcgcgcgca cgggcgaggc gctggtcaag gagatgcgcg
     3661 acgcggtcga gaaggtgccc ggcgtgtcca cgctgttcac cacgccgctc ggcatgcgga
     3721 tcgacgaggg gctcggcgga accccggcgg acatctcggt ccggatcttc ggcccggacg
     3781 cacagcagct cgcggacctc gcggagtctg ccgcggccac gatccgcgcg atcgacggcc
     3841 tggaggacgt gcgggccgag ccgctggtcg gactgccgca gctgaccgtc caggtcgatc
     3901 gagaggcagc ggcgcgcgtc gggctcacgc cgggagacgt ggtcgaggcg gtgcagatcg
     3961 ggctcgccgg gcaagaggtg tccgagatct ggatcggcca gcgccggttc gggctgacgg
     4021 tcaagctctc gccggacttc cggcgcgacg ccgcgacgct cgccgcgctg aggatcgacg
     4081 gccacgacgg cagccgcatc ccgctcggca agctggccag gatcgagccg gcgttcgggc
     4141 tcggcaccat caagcgcgag gccggcagcc gccggatcgc cgtcgaggcc agcgtgtccg
     4201 gacgcgacct gggctcggcc gcgcgcgatg tgcgcgatgc gctcgcc
//
LOCUS       DASVOR010000395         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_237253,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000395 DASVOR010000000
VERSION     DASVOR010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237253"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1833)
                     /locus_tag="VF516_08005"
     CDS             complement(<1..1833)
                     /locus_tag="VF516_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8107658.1"
                     /translation="MSSSAPERLTRKTTSLAELLARRVRDTPDREAYRYPVGDQWRSM
                     TWRQSGERVRAISAGLLSLGLDREDRVGILCNTRVEWLLADLGVLSAGGATTTVYPSS
                     TAEECAYILADSAARYAFAEDAAQLAKLAAHRAGLPRLARVILIDGAPAEAQRDWVMT
                     LSELEAAGAAHLERDPRAVDDVVAGIQGGHLATLIYTSGTTGKPKGVRLLHECWAYCA
                     DAIDATRLWVADDVQYLWLPMSHSFGKVLMAGHIASGSVTAVDGRIPKLVDNLAAVKP
                     TLMAAVPRIFEKVYNRILENAKQGGAIKHRIFQWAMAVGRAGSQVRQRGAAPSGLLAL
                     QLRLAERLVFSKIKTTFGGRVKYFISGSAPLSREIAEFFHACGVLILEGYGLTETSAA
                     SFVNRPTRFAFGTVGVPLPGTEVRLAPEDGEILIKSPGVMNGYHNLPDQTAEALTSDG
                     WMRTGDIGEIDRDGFLRITDRKKDLIKTSGGKYIAPQSIEGRLKTVCPYISQVLIHGD
                     KRNFVTALVSLDEEATMKWAREHGLGGKSYSELVKADQVKQLLAPYFDQVNKTLARYE
                     TVKQFAVLPRDLSVDEGELTPSLKVKRKVVEKRYAALLDKMYEGA"
BASE COUNT          273 a          687 c          702 g          306 t
ORIGIN      
        1 cgcgccctcg tacatcttgt cgagcagcgc ggcgtagcgc ttttcgacca ccttgcgctt
       61 gaccttgagg ctcggcgtca gctcgccctc gtcgaccgac agatcccggg gcaggacggc
      121 gaattgcttg acggtttcat atctggccag ggtcttgttg acctggtcga agtacggcgc
      181 gagcagctgc ttgacctgat cggccttcac cagctcgctg tacgacttgc cgcccaggcc
      241 gtgctcgcgg gcccacttca tcgtggcctc ctcgtcgagc gagaccagcg cggtgacgaa
      301 gttgcgcttg tcgccgtgga tgaggacctg gctgatgtac gggcacaccg tcttgagcct
      361 gccctcgatc gattgcggcg cgatgtactt gccgcccgag gtcttgatca ggtccttctt
      421 gcgatcggtg atccgcagga atccgtcgcg atcgatctcg ccgatgtcgc cggtgcgcat
      481 ccagccgtca ctggtcagcg cctcggcggt ctggtccggc aggttgtggt agccgttcat
      541 cacgcccggc gacttgatca ggatctcgcc gtcctcgggc gcgagtcgga cctcggtgcc
      601 cggcagcggg acgccgaccg tgccgaacgc gaaccgggtc ggccggttga cgaacgacgc
      661 ggccgaggtc tcggtcaggc cgtagccctc gaggatcagc acgccgcagg cgtggaagaa
      721 ctcggcgatc tcgcgcgaca gcggcgccga gcccgagatg aagtacttca cccggccgcc
      781 gaacgtggtc ttgatcttcg agaacaccag gcgctcggcg agccggagct ggagcgcgag
      841 caggccgctc ggcgccgcgc cgcgctggcg gacctgcgag ccggcccggc cgaccgccat
      901 cgcccactgg aagatccggt gcttgatcgc cccgccctgc ttggcgttct ccaggatccg
      961 gttgtagacc ttctcgaaga tccgcggcac ggcggccatc agggtcggct tgaccgcggc
     1021 gaggttgtcg accagcttgg ggatccggcc gtcgaccgcg gtgaccgagc ccgacgcgat
     1081 gtggccggcc atcagcacct tgccgaacga gtgcgacatc ggcagccaca ggtactggac
     1141 gtcgtcggcc acccacagcc gggtcgcgtc gatcgcgtcg gcgcagtacg cccagcactc
     1201 gtggagcagc cgcacgccct tgggcttgcc ggtggtgccc gaggtgtaga tcagggtcgc
     1261 gaggtgcccg ccctggatgc cggcgacgac gtcgtcgacc gcgcgcggat cgcgctcgag
     1321 gtgcgccgcg cccgccgcct cgagctcgga cagcgtcatg acccagtcgc gctgggcctc
     1381 ggccggcgcg ccgtcgatca ggatcacgcg ggcgagccgg ggcagcccgg cgcggtgcgc
     1441 ggccagcttg gcgagctgcg cggcgtcctc ggcgaacgcg tagcgcgccg ccgagtcggc
     1501 gaggatgtag gcgcactcct cggcggtgct cgacgggtac accgtggtgg tcgcgccgcc
     1561 ggccgacagc acgccgagat ccgcgagcag ccactcgacc cgggtgttgc acaggatgcc
     1621 gacccggtcc tcgcgatcga gcccgagcga caagaggccc gccgagatcg cgcggacccg
     1681 ctcgcccgac tgccgccacg tcatcgatcg ccactggtcg ccgaccgggt agcggtaggc
     1741 ctcgcgatcc ggcgtgtcgc gcacgcggcg ggcgagcaac tcggcgagcg aggtggtctt
     1801 ccgggtgaga cgctcggggg cagacgacga catgccgacg catcatgcca agttgcacgc
     1861 ggccttgcac ccgcggcgcc tcgcgttcgc ggggtgggcg cgcgggcctg ggcgcgacgg
     1921 cgccgcccgg tgcggcgtgg gcctcgggcc cgaactgacg tacgctcg
//
LOCUS       DASVOR010000396         3749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_296024,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000396 DASVOR010000000
VERSION     DASVOR010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3749
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296024"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..2170
                     /locus_tag="VF516_08010"
     CDS             17..2170
                     /locus_tag="VF516_08010"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8107659.1"
                     /translation="MKRTALLVLALAGCGSATTASQPANRQPQAGPRPPIAERRPYVV
                     KSPGGDREDPYYWLRDDTRKRPDMLGYLEAENGYTRAILDPVKPLEDKLLAELKSHVQ
                     EDDTSAPVFDDGYWYYTRFSAGQEQPVYVRKQGSKDAAEEVVLDGNELARGHAFFAFG
                     GHEVSRDGHLVAWTDDTVGRRQFVLHVKDLRTGQVLPDTASNVAGLVQWANDGKTLFY
                     VGKDPTTLREDRVFRHTLGERSDAQVFHEPDGQYYVSIQPTKSRRYVMIHLRATTHTE
                     VRLIDADHPTAADRVVLPRQRDHIYDLDHLDGRFVIRTNAGAKNFRLVEVAEDRVSDR
                     GAWRDVIPASADTLVEDFALSRHFIAATVRTGGLRKVEVLPARGAAFFIDAGEPAYMM
                     FADDLPDPDSRVVRYEYVSQVTPRTTFELDVATRAKTQLRVQPVPGYDPANYAAEYLH
                     ARASDGTQIPISVVYRKDTRRDGTAPLLVYGYGSYGFSSEPTFDSSRQALLDRGWVFA
                     TAHVRGGQEMGRAWYEDGKLMHKRNTFTDFIAATEHLVAGGYGARDQVYAQGGSAGGL
                     LMGAVLNLRPDLYRGVIAAVPFVDVVTTMLDESIPLTTNEFDEWGNPKDKAAYDYMLS
                     YSPYDNVKPQAYPSIYVRTGLWDSQVQYFEPAKWVAKLRATKTDENLVLLDTDMAAGH
                     GGKSGRFDRLHDVARMMTFLLLVHDRPDGRPHPGR"
     gene            complement(2181..2567)
                     /locus_tag="VF516_08015"
     CDS             complement(2181..2567)
                     /locus_tag="VF516_08015"
                     /inference="COORDINATES: protein motif:HMM:NF022703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3060 domain-containing protein"
                     /protein_id="HEX8107660.1"
                     /translation="MKTGIALLIAIAPAIAPMTARADKLFIKGKGAAWDCAKDPVVNI
                     NHGEGTYTFTGKCKTINVNGGDNKLTIESVDTINVNGGDNTVAIGTADKINVNGSDNK
                     VTYRTAKSGKVTARSLGGGNTVEQAK"
     gene            complement(2621..>3749)
                     /locus_tag="VF516_08020"
     CDS             complement(2621..>3749)
                     /locus_tag="VF516_08020"
                     /inference="COORDINATES: protein motif:HMM:NF042530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HEX8107661.1"
                     /translation="VNPGAWLMATAKRRAIDRLRRARVIERSHEQLGAGAGEAAAADL
                     VAALDAALDGDPGDDLLRLVFTACHPVLPPDGRVALTLRLVGGLTTPEIARAFLVSEP
                     TMAQRIVRAKRTLAEAGVAFEVPRGPELAERLASVLEVIYLVFNEGYAASSGDDWVRP
                     ALCEDGLRLGRILAQLIPDEPEVHGLVALMEIQASRLRARTGPAGEPVLLLDQNRARW
                     DHVLIRRGLAALDRAIALGGARGPYALQAAIAACHARAVVAEDTDWPRIAALYTALTA
                     LIPSPVIELNRAVAVGMAFGPAAGLELVDALAGDPSLAHYHLLPSVRGDLLARLGRLE
                     EARRELERAADLAGNARERDLLLARAAACDRPDVDRGADSS"
BASE COUNT          556 a         1371 c         1302 g          520 t
ORIGIN      
        1 tgatactgcg gaaggcatga agcgcaccgc attgctcgtc ctcgcgctcg ccggctgcgg
       61 ttcggccacg acggcgtcgc aacccgccaa ccgccagccg caggccggcc cgcggccgcc
      121 gatcgccgag cgccggccct acgtggtcaa gtcgcccggc ggcgatcgcg aggatccgta
      181 ctactggctg cgcgacgaca cgcgcaagcg ccccgacatg ctcggctacc tcgaggccga
      241 gaacgggtac acccgggcga tcctcgatcc ggtcaagccg ctcgaggaca agctgctcgc
      301 cgagctcaag agccacgtcc aggaggacga cacgagcgcg ccggtgttcg acgacggcta
      361 ctggtactac acgcggttct cggccggcca ggagcagccg gtgtacgtcc gcaagcaggg
      421 cagcaaggac gctgccgagg aggtcgtgct cgacggcaac gagctcgcgc gcggccacgc
      481 gttcttcgcg ttcggcggcc acgaggtcag ccgcgacggt cacctggtgg cgtggaccga
      541 cgacacggtc ggccgccgcc agttcgtgct gcacgtcaag gatctgcgca ccggccaggt
      601 cctgcccgac accgcgagca acgtcgccgg cctcgtgcag tgggccaacg acggaaagac
      661 gctgttctac gtcggcaagg acccgaccac gctgcgcgag gaccgggtgt tccgccacac
      721 gctcggcgag cgcagcgacg cgcaggtgtt ccacgagccg gacggccagt actacgtatc
      781 gatccagccc accaagtcgc ggcgctacgt gatgatccac ctgcgcgcga ccacccacac
      841 cgaggtccgc ctgatcgatg ccgatcaccc gacggcggcc gaccgggtcg tgctgccgcg
      901 ccagcgcgat cacatctacg acctcgatca cctcgacggc cggttcgtga tccggaccaa
      961 cgccggcgcc aagaacttcc ggctggtcga ggtcgccgag gaccgggtct ccgatcgcgg
     1021 cgcatggcgc gacgtgatcc cggccagcgc ggacaccctg gtcgaggact tcgcgctgtc
     1081 gcgccacttc atcgccgcca cggtgcgcac cggcgggctg cgcaaggtcg aggtcctgcc
     1141 ggcccggggc gcggcgttct tcatcgacgc cggcgagccg gcgtacatga tgttcgccga
     1201 cgacctgccc gatccggact cccgggtggt gcgctacgag tacgtgtcgc aggtgacgcc
     1261 gcggaccacg ttcgagctcg acgtcgcgac ccgcgcgaag acccagctcc gggtccagcc
     1321 ggtgcccggc tacgacccgg cgaactacgc cgccgagtac ctgcacgcgc gcgccagcga
     1381 tggcacgcag atcccgatct cggtcgtgta ccgcaaggac acccggcgcg acggcaccgc
     1441 gccgctcctg gtctacggct acggctcgta cgggttctcg tcggagccga ccttcgactc
     1501 cagccgccag gcgctgctcg accgcggctg ggtgttcgcc accgcgcacg tccgcggcgg
     1561 gcaggagatg gggcgggcct ggtacgagga cggcaagctg atgcataaac gtaacacgtt
     1621 caccgacttc atcgccgcga ccgagcacct ggtcgccggc ggttacggcg cgcgcgacca
     1681 ggtctacgcc cagggcggca gcgccggcgg tctgctgatg ggcgcggtgc tcaacctgcg
     1741 gcccgacctg taccgcggcg tgatcgcggc ggtgccgttc gtcgacgtcg tgaccaccat
     1801 gctcgacgag tcgatcccgc tcaccaccaa cgagttcgac gagtggggca acccgaagga
     1861 caaggcggcc tacgactaca tgctgagcta ctcgccgtac gacaacgtca agccccaggc
     1921 ctacccgtcg atctacgtcc gcaccgggct gtgggacagc caggtccagt acttcgagcc
     1981 ggccaagtgg gtcgccaagc tgcgggcgac caagaccgac gagaacctgg tgttgctcga
     2041 caccgacatg gccgcgggcc acggcggcaa gtcgggccgg ttcgaccggc tgcacgacgt
     2101 cgcgcgcatg atgacgttcc tgctgctggt ccacgaccgc ccggacggcc ggccccaccc
     2161 gggccggtag ccgcgcggcg ctacttcgcc tgctcgacgg tgttgccgcc gcccagcgag
     2221 cgggcggtca ccttgccgga cttcgcggtc ctgtaggtga ccttgttgtc gctgccgttg
     2281 acgttgatct tgtcggcggt gccgatcgcg accgtgttgt cgccgccgtt gacgttgatc
     2341 gtgtcgacgc tctcgatcgt cagcttgttg tcgccgccgt tgacgttgat cgtcttgcac
     2401 ttgccggtga acgtgtaggt cccctcgccg tggttgatgt tgaccacggg gtccttggca
     2461 cagtcccacg cggcgccctt gcccttgatg aacagcttgt cggcccgggc ggtcatcggc
     2521 gcgatcgccg gcgcgatcgc gatcagcagc gcaattcctg tcttcatggc gtctccctcc
     2581 acatcctcgc cccggacggc caaggcggtg gccatgcatc ctaactggaa tccgcgccac
     2641 gatcgacatc agggcggtca cacgccgcgg cgcgggcgag cagcagatcg cgctccctgg
     2701 cattgccggc gagatcggcg gcgcgctcca gctcccggcg ggcctcctcg agccggccga
     2761 gccgggcgag caggtcgccg cgcacgctgg gcagcaggtg atagtgggcg agcgacggat
     2821 cgccggccag cgcgtcgacc agctccaggc cggcggccgg cccgaacgcc atcccgaccg
     2881 cgaccgcgcg gttgagctcg atcaccggcg acgggatgag cgcggtgagc gcggtgtaca
     2941 gcgcggcgat ccgcggccag tcggtgtcct cggcgacgac cgcccgggcg tggcaggcgg
     3001 cgatcgcggc ctgcagggcg tacggcccgc gcgcgccgcc gagcgcgatc gcacgatcga
     3061 gcgcggcgag cccgcgccgg atcagcacgt ggtcccagcg cgcgcggttc tgatcgagca
     3121 gcagcacggg ctcgccggcc ggtccggtcc gggcgcgcag ccgcgacgcc tggatctcca
     3181 tcagcgcgac caggccgtgg acctcgggct catccggaat cagctgggcg aggatccttc
     3241 cgagccgaag tccgtcctcg cacagcgccg gccggaccca gtcgtcgccc gagctcgcgg
     3301 cgtagccctc gttgaacacc aggtagatca cctcgagcac cgaggccagc cgctcggcga
     3361 gctcgggccc gcgcgggacc tcgaacgcga ccccggcctc ggcgagcgtc cgcttggcgc
     3421 ggacgatccg ctgcgccatg gtcggctcgg acaccaggaa cgcccgggcg atctccggcg
     3481 tggtcagccc gccgaccagc cggagggtga gggcgacccg gccgtccggc ggcagcaccg
     3541 ggtggcaggc ggtgaacacc aggcgcagca ggtcgtcgcc gggatcgcca tcgagcgcgg
     3601 cgtccagcgc ggcgaccagg tcggcggccg cggcctcccc ggcgccggcc ccgagctgct
     3661 cgtggctgcg ctcgatgacg cgggcccggc gcagccggtc gatcgcacgg cgcttggcgg
     3721 tcgccatcag ccaggcgccc gggttgacc
//
LOCUS       DASVOR010000397         5042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_149767,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000397 DASVOR010000000
VERSION     DASVOR010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149767"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            838..1671
                     /locus_tag="VF516_08025"
     CDS             838..1671
                     /locus_tag="VF516_08025"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX8107662.1"
                     /translation="MTFLDLVGAAHRTAQFPVVRFGGQLAPKGMITSETVFERLLTLS
                     PEELDVVRKQVRKQADWLAGSRDREGAATASRLRGTVEFLDRLPALPGPAVETWTTAP
                     EPDAQQLAQHLQRCLWLFEHDLTACAWVNCFRWDTHSDNYAQQGRASSALLPVLARFV
                     EELAKRTNSHGSLLDQVRIIAGSELGRFPKLNSERGKHHFPEVPMLFMGAGIPGGRAF
                     GRTGRMMEALPIDLNTGLATGNGGSVMTLDDVGATLLRSVGVDPELYGNSGRHLPFLV
                     P"
     gene            2273..3223
                     /locus_tag="VF516_08030"
     CDS             2273..3223
                     /locus_tag="VF516_08030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107663.1"
                     /translation="MPVCDGCHARLDYGAQFFEGITAGFHYNSQFLPRAGSTMNMYIH
                     DQFDLRGRGEQTPAGFAELAVSQPEFGACMSKRVAAHVFGRQATEGEVASLLEVFKTR
                     PTYRALLTSALVSYAERRLWSADERGEIGHADPAAPQLGLGALVEQYCSDCHDTGGVA
                     EKPWSSSRALADHGAMVQALDSIGDRRMPPASNPLDPTVRERFVALLAERLWPDAEAR
                     NAALDNYSGRLNDVEVHRRGSTMKIIAALAGVNLDDAIAKVGPLYAGSGRNTQEYTPD
                     FALNTVIDGIAACKLAGAKDLADCVERATRLDTILPVAPR"
     gene            complement(3242..3412)
                     /locus_tag="VF516_08035"
     CDS             complement(3242..3412)
                     /locus_tag="VF516_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107664.1"
                     /translation="MRSPRGRATPPPRARLGGLRQVVTDWLGEVPRRPLDVTLRDTIG
                     ARSVAASADERA"
     gene            complement(3421..4116)
                     /locus_tag="VF516_08040"
     CDS             complement(3421..4116)
                     /locus_tag="VF516_08040"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HEX8107665.1"
                     /translation="MNLAGYRNIVVLTGAGISQAAGLPTYRGAGGLWSDPELVKLSDV
                     SALATHRAEACGMFWRFRQAIAAVQPTAAHRALAAFERALPAGASFLIVTQNVDGLHQ
                     KAGSVRVCEYHGTLARWRCEVCGAEHEPPDGDPPVCCGLFMRPSAVLFGELIPADAEH
                     TAKRALRDCDLFVAIGTSGTVWPAANFVRWAELNGARRVLLNREIDDEARDTYGECHA
                     GTADELVPQLFAV"
     gene            4343..5038
                     /locus_tag="VF516_08045"
     CDS             4343..5038
                     /locus_tag="VF516_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107666.1"
                     /translation="MAGDPATGRRACTLAAVAMVAALCAPGCAITTMTLETPPHARVT
                     MATSVGEGREIALVVPFADRRATPQRCGMKKNGYNMDTADILCAEPPNQWLAALLAEE
                     LRKAGFRVAADPRQAGPGALRLEGTLTQFFLEPDVGFFTFSPEADIELRLVASAPSGL
                     VAERRFYCKGRETALSGLEENFRLAADSAVRDMLVRVVAAVAELVRQYPDIGLPPRVG
                     ALAARRTGGEVAR"
BASE COUNT          751 a         1731 c         1776 g          784 t
ORIGIN      
        1 ccgccgtcga agccggcacg cgcgacgccg ccgcaccagc cccacagcca cggccgccgc
       61 gatctgccga gcaccggcag cagcaagcgc tggcggcggc gcgggggagg cggccgcggg
      121 cctgcggcga gcgcgtagat gagtcgtcag aatcgatctg ttcgaggaac tccggcttga
      181 ggctccttgc cattcgcttt cgccacgccc tgtagtcgca tctcgacatg cgccctctgt
      241 gccaacgtga ctgagacagc gacctcggtc ttgcccttcg gggccgaatg cctcgtttcc
      301 agcatgcgca ttgccgccac agttgtcgga acagcaggcg tagccgcaca ccgcccggcc
      361 ttcgatccag tagctcccga cacccgccga cacgtgatgg tcttttcttg ttccattcta
      421 cggtgtggct tcttacgctt cgcgagttga cactgccgca aaacactccg ctgctggcct
      481 actcccgccg caaatccaag gccgggccac acaagatcgc tactttgtcc tggcgccccg
      541 ctcatcttgc gtcgctgggg atcagtccac tagtctctgc ccatgcttcg aaggcgcttt
      601 cttagcgggt tcgcggctgg actcgcgagc ctcgcggtcg tgccgaggtt gagccgggcc
      661 accgcaccgc gaaggacgcc acgggcgagc tgacgctggg gcgcaacatg gtgtcgctcg
      721 caccgtgggc cgggcgccgc cgtcctgcgc ggtgtgctca cgagcagcgt cgggcacgac
      781 ccggcgatat accagctcct gctcatgcga cgtgggattc cacttggcgg caagcacgtg
      841 acgttcctcg acctcgtggg agccgcacat cggaccgcgc agttccccgt ggtccggttc
      901 gggggccagt tagctcccaa ggggatgatc acgagcgaga ccgtgttcga acgcctgctg
      961 accttgagcc ccgaagagct cgacgtggtt cgcaaacagg tccgcaagca ggcggattgg
     1021 ctcgctggtt cacgcgaccg cgaaggcgca gccaccgcat cgcgcctgcg cggcacggtc
     1081 gagttcctcg accgcttgcc tgcgctgcca ggtccggcgg tcgagacgtg gacgaccgca
     1141 cccgaacccg atgcacagca actcgcgcag cacctccagc gatgcttgtg gttgttcgag
     1201 cacgacctga ccgcgtgtgc gtgggtcaat tgcttccgat gggatactca cagcgataac
     1261 tacgcgcagc agggtcgggc ctcgtcggcg ctgttgccgg tcctcgcgcg gtttgtcgag
     1321 gagctcgcga agcgaacgaa ctcccacgga tcgctgctcg atcaggtcag gatcatcgcc
     1381 gggtccgagc tcggacggtt tcccaagctc aacagcgaac gcggcaagca ccacttcccg
     1441 gaggtcccga tgctgttcat gggcgcaggc attccggggg gccgcgcgtt cggacgcacg
     1501 ggccgcatga tggaggcgct gccgatagac ttgaataccg ggctcgccac cggaaacggt
     1561 ggttcggtca tgacgctcga cgacgtcggc gcgacgctgt tgcgatcggt gggcgtcgac
     1621 ccggagctgt acggcaacag cgggcggcat ctgccgtttc tggtgccgtg atgcgcctcg
     1681 ggcttgttgt tgtcgtgtgc ttccttgcgg cctgtcagaa ccacgcacag cgatcggtca
     1741 agcccgacgc ccacgccgat gtcggatccg gtggtgaagc gacaaccagg gtcgatccca
     1801 aacgcgcgaa caagatcgcg gccgacttgt tcctcctcgc gtcgaatcgc gtgccatcgc
     1861 gccgagagct caccgaggca gctgcagcgc tcgcggctgg agatcgggag ctccgacgcg
     1921 ttcgtcgcgg ggctcgtcgc gaccgacgcg agcgccgagt tcgcgccgtt cgcactgttc
     1981 ccgagcgggt ccgcccgtgg tggagaccgg tgcgccggcg gagctgttgt cggggcccgg
     2041 ttgggtcgag gggccctcgc cgcgcccgga gatccgccca ggggcgcacg ccggcctgct
     2101 gtcgtctccc gaggtggcga ccttgggctg cgagcctcgc gaacggctga tggcgatctc
     2161 ggacttgatc tggtgtgtcg cgccccggag cgtcagcgtc accgccgata cggtgttccg
     2221 gctccccacc aactttcgca ccccgatgca aggctggcag ttgttggcga gcatgccggt
     2281 ctgcgacggc tgccacgcac ggctggacta tggcgcgcag ttcttcgagg ggatcaccgc
     2341 cggctttcac tataactctc agttcttgcc gcgcgccggc agcacgatga acatgtacat
     2401 ccacgaccag ttcgacctcc gcggtcgcgg cgagcagact cccgccgggt tcgcggagct
     2461 cgcagtgtcg caaccggagt tcggcgcgtg catgagcaag cgagtcgcgg ctcatgtgtt
     2521 cggccggcag gcgaccgagg gcgaggtcgc atcgctgctc gaggtgttca agacgcggcc
     2581 tacgtaccgc gcgttgctga cctcggcgct ggtgtcgtac gcggagcgcc ggctgtggag
     2641 tgccgacgaa cgcggcgaga tcggtcatgc ggatccagcg gcgccgcagc tcggcctcgg
     2701 cgctctggtc gagcagtact gctccgattg ccacgacacc ggcggcgttg ccgaaaagcc
     2761 atggagctcg agccgagcgc tcgccgacca cggcgcgatg gtccaggcgc tggattccat
     2821 cggggaccgg cggatgccgc ctgccagcaa tccgctcgat cccaccgtgc gggagcgatt
     2881 cgtcgcgctg ctcgccgaac ggctctggcc ggacgccgag gcccggaacg ccgcgctgga
     2941 caactacagc ggacggctga acgacgtcga ggtccatcga cgggggtcga cgatgaagat
     3001 catcgcagct ctcgccggtg tcaacctcga cgatgccatc gcgaaggtcg gtccgctcta
     3061 cgccggcagt gggcgcaaca ctcaggagta cacacccgat ttcgcgctca acaccgtgat
     3121 cgacggcatc gcggcctgca agctcgccgg cgcgaaggat ctcgccgact gcgtcgagcg
     3181 agcgacccgc ctggacacga tccttccggt cgcgccgcgc tgaccggtcc gcctccggcg
     3241 atcaggcgcg ctcgtcggcc gacgccgcga cgctgcgcgc gccgatcgta tcgcgcagcg
     3301 tgacgtcgag cgggcggcgt ggaacctcgc cgagccagtc ggtgacgacc tggcggagcc
     3361 cgccgagccg ggcgcgcggg gggggcgtgg cgcggcccct cggcgagcgc atagcccggc
     3421 tcacaccgcg aacagctgcg ggaccagctc gtcggcggtc ccggcgtggc actcgccgta
     3481 ggtgtcgcgc gcctcgtcgt cgatctcccg gttgagcagg acgcggcgcg cgccgttgag
     3541 ctcggcccag cgcacgaagt tcgccgccgg ccacaccgtg cccgaggtgc cgatcgcgac
     3601 gaacagatcg cagtcgcgca gcgcgcgctt ggcggtgtgc tcggcgtccg ccgggatcag
     3661 ctcgccgaac agcaccgccg acgggcgcat gaacaggccg cagcacaccg gcggatcgcc
     3721 gtcgggcggc tcgtgctccg cgccgcacac ctcgcagcgc cagcgcgcga gcgtgccgtg
     3781 gtactcgcag acccggaccg atccggcctt ctggtgcagg ccgtcgacgt tctgggtgac
     3841 gatcaggaag ctcgccccgg cgggcagcgc gcgctcgaac gcggcgagcg cgcggtgggc
     3901 tgccgtcggc tggactgcgg cgatcgcctg gcgaaaccgc cagaacatgc cgcacgcctc
     3961 ggcgcggtgg gtggccagcg cggagacgtc ggacagcttg accagctcgg gatccgacca
     4021 cagcccgccg gcgccgcgat aggtcggcag gccggccgcc tgcgagatgc cggcgccggt
     4081 cagcacgacg atgttgcggt agcccgccag gttcatcagc ttagtgtaca gacgacaata
     4141 atcagcgcaa gcggaccgcc ccaacctggg gagcggtccg ccgcatttgg tgcggcagga
     4201 ttcctcacgc cggtctcaca tgcgatatgg tcgctgtccc gcatggactg cgctgtgagc
     4261 gtgcagacgg gggaaaggca ggacgacgat gtgtcgaggt gaaccatcca gcgacggggt
     4321 cgcgcgccgt cgcgctgtgg acatggccgg agatccagcg accggccggc gcgcgtgcac
     4381 gctggccgcg gtcgcgatgg tggccgcgct gtgcgcgccc ggctgtgcga tcacgacgat
     4441 gacgctggag acgccgcccc atgcgcgggt cacgatggcg accagcgtcg gcgagggccg
     4501 cgagatcgcg ctggtcgttc cgttcgcgga ccggcgggcg acgccgcagc gctgcgggat
     4561 gaagaagaac ggctacaaca tggacaccgc ggatatcctg tgtgcggagc cgccgaacca
     4621 gtggctcgcc gcgctgctcg ccgaggagct gcgcaaggcg gggttccggg tcgcggccga
     4681 cccgcgccag gccggaccgg gcgcgctgcg gctcgagggt acgctcaccc agttcttcct
     4741 cgagcccgac gtcggcttct tcacgttctc cccggaggcc gacatcgagc tccggctggt
     4801 ggcgagcgcg ccgtcggggc tggtcgcgga gcgcaggttc tactgcaagg gccgggagac
     4861 cgcgctgtcc ggcctggagg agaacttccg gctggcggcg gactccgcgg tccgggacat
     4921 gctggtccgg gtcgtggccg cggtggccga gctggtccgg cagtatccgg acatcggcct
     4981 gccgccgcgc gtgggggccc tggccgcgcg ccgcaccggc ggggaggtgg cgcgatgagg
     5041 gc
//
LOCUS       DASVOR010000398         1813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_62140,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000398 DASVOR010000000
VERSION     DASVOR010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1813
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62140"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            320..880
                     /locus_tag="VF516_08050"
     CDS             320..880
                     /locus_tag="VF516_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107667.1"
                     /translation="MELQPEWNEFLRLLKHHGVRFVLVGGLAVGIHGHERYTKDLDLL
                     VDPSEANARRLGKALVEFGFTTTGRAWRRLTKPYQILTLGVEPVRIDVLTSIGGVAFR
                     AVWKGRGVVATRAGDIPVIGLTELRINKLATGRPQDLADVAQLDAVARVKRAPEAQER
                     SADVRSRPTVPRGSSARNRGVRRPRR"
     gene            complement(995..1624)
                     /locus_tag="VF516_08055"
     CDS             complement(995..1624)
                     /locus_tag="VF516_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107668.1"
                     /translation="MVNQPAPPIAGEGEFWAGVANAQRFFMGNAEVQYALQKLTAVLD
                     AAGIPYAIAGAMALNHYGYRRVTVDVDVLLTRDGLERLKHDVLGRGYVEKFPGSKGIR
                     DIEHNVAIDVLLAGEYPGDGKPKPVRFPDPAIVAIRGERGAFLPLETLIELKLASGLS
                     APHRLKDLADVLELIRAAGLPATLADRLDPSVRAKYTELWTAAQTRDDE"
BASE COUNT          299 a          614 c          615 g          285 t
ORIGIN      
        1 tgcagtacaa cttcgtgatc tccaccaacg agcgcgccgt ccggctctgg cagtccatgg
       61 gcttctcgat cgtcggccgc ctccccggcg cgttcgtcca cccgaccctc ggcgacgtcg
      121 acgcgctcgt catgtaccag acgctgtagc cgtgtggggg ctccacgggc ctggtatgct
      181 gcgcccgtga agcgggtcgc gacctggacg acgttcgacg aagccaccgc ggcccagatc
      241 cgcgaggccc tgtcacgatc accggaacag cgactcgcga tggtccagcg gatccgcgaa
      301 gcgcactggg ggctgttcgg tggagctcca accggaatgg aacgagtttc tacgtttact
      361 gaagcaccat ggcgtccgct tcgtgctggt gggcggcctc gccgtcggca ttcacggcca
      421 cgaacgatac acgaaggacc tcgacctgct cgtcgatccc agcgaggcaa acgcccggcg
      481 tctcggtaaa gcgctggtcg agttcggctt caccaccacg gggcgcgcct ggcgccggtt
      541 gacgaagccg taccagatcc tgacgctcgg cgtggagcca gtccgcatcg atgtcctgac
      601 cagcatcggc ggggtggcgt tccgcgcggt ctggaagggt cgcggggtcg tcgccacgcg
      661 cgctggtgat atcccggtca tcgggctgac cgagctccgc atcaacaagc tcgcgacggg
      721 ccggccgcag gatctcgcag acgtggctca gctcgacgca gttgcgcgcg tcaagcgagc
      781 ccccgaggcc caggaacgca gcgcggacgt gcgctccagg ccaaccgtac cgagaggcag
      841 cagcgcgcgc aatcgtggtg ttcgacgccc gcgaagataa tcgccggcag gaatcggcca
      901 cgacgagtcg cggaacacac agaaagcgat cgccgcctgc cactgtttcc ggaacgtccg
      961 aaggagcaat tgtccggcgg tgcccttggg cgcgtcattc gtcgtcgcgg gtctgcgcgg
     1021 cggtccacag ctcggtgtac ttggcccgga ccgatggatc gaggcggtcg gcgagggtcg
     1081 caggcaagcc agcggcgcgg atcagctcca ggacgtcggc gagatccttg aggcgatgcg
     1141 gcgcggacag ccccgaagcg agcttgagct cgatcagggt ctccagcggc aggaacgcgc
     1201 cgcgctcacc ccggatcgcc acgatcgcag ggtccggaaa ccggacaggc ttgggcttcc
     1261 cgtcaccggg gtactcacca gcgaggagca cgtcgatggc gacgttgtgc tcgatatcgc
     1321 gaatgccctt gcttcctgga aatttctcga cgtagccacg gccgagcaca tcgtgcttga
     1381 gccgctccaa gccgtcgcgg gtcaggagaa catcgacatc gacggtgacc cggcggtacc
     1441 cgtagtgatt gagcgccatg gccccggcga tcgcgtaggg aatgccagcc gcgtcgagca
     1501 cggcggtgag cttctgcagc gcgtactgga cctcggcatt ccccatgaag aacctctgtg
     1561 cgttggcaac tcccgcccag aactccccct cgccggcgat cggaggagct ggctggttca
     1621 ccatgtgatg aagatagcac cgggctgcca tctggaacgc ggttgcgacg cgcggtgcta
     1681 cggtagcggc atgaccaccg catcgccgcc gcgtccggcc cgggttcctg cgtcctgctt
     1741 cgcgccggcg atcgcggccg ccgcgctgtc ggcgtgcggc gggaccagcc cgccgtcgcg
     1801 gcccggcgac gcg
//
LOCUS       DASVOR010000399         7210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_237321,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000399 DASVOR010000000
VERSION     DASVOR010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237321"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VF516_08060"
     CDS             complement(<1..857)
                     /locus_tag="VF516_08060"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX8107669.1"
                     /translation="MIREFAFPGTRPRWAPDRVVDIQHLSLVLDVDPAARSVAGTATL
                     RCRALADDTRSVELDAVELTIERVTVGGEPAAFRHDGRKLRIELPVPPQGAAAGAELA
                     ITVAYRGAPRRGLYFIAPDDAYPGKPVQVWSQGQDEDSRYWFPCFDAPHEKATSELTV
                     TVPAHLFAVSNGALVSDRTEGDRRTLHWRLDVPHSCYLITLAVGDFATIETRWRDVPV
                     VYYVERGREAAAERTLARTPQMMELFSERFGVPYPYPRYAQVFVADFIFGGMENTSAT
                     TLTDTVLLD"
     gene            complement(1006..1806)
                     /locus_tag="VF516_08065"
     CDS             complement(1006..1806)
                     /locus_tag="VF516_08065"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8107670.1"
                     /translation="MPPAASHVDSHLSGVDDRLLEPETRYEMYDGELVYVSPADPPHG
                     ERHLQLAALIEAHTGFEFEAAADLLTRTSKVDDIAPDVSVYPAAPDPRTGGRQLEQLA
                     FEIISAQSLGRATTKAVKLTARGVRRVFAIDVERSRVLEWSTDRGTWHELDAAGHIAD
                     PALEVPLPIEAMVHAAKADDAVARALVARHNLVIEAVRAEDRAAGKAEGKIEGKIEAL
                     IAILAARHIDPDSAARERILRERDPRRLDRWIARAAISATIAEVLASE"
     gene            complement(2038..2328)
                     /locus_tag="VF516_08070"
     CDS             complement(2038..2328)
                     /locus_tag="VF516_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107671.1"
                     /translation="MNEPPNAGAAAGCADAATGGDCDHRRLHARLTQLTDTVFEEILL
                     LAPAPRGHIAPATAPLATRILHVAQLAAGNQALCRSLAALLDERARWTRSGT"
     gene            complement(2325..3464)
                     /locus_tag="VF516_08075"
     CDS             complement(2325..3464)
                     /locus_tag="VF516_08075"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8107672.1"
                     /translation="MGLAPATVVPRSDGEARVFLKDTVPDGGRALAQYRAWLSSALDG
                     ASLARLYADLFGRPLQHDVDVTGSFQDVLHRTLESCAQRGRPLMQLFARAVRARADRK
                     EIVEQLAAVLGLAPVIEIAGLLAHHGCPETLCDEIAQALAMDRDASYLDHGHSGRAAW
                     TDALVWLEELPAAAGRPPPLLDYLERLTTAWSTSHDGIPAEIARWLDDHRGPREGKRG
                     DAPPAGASGNRAPGQQIRPASIPSSARYDLFLAHSPGGRPSAHALYSLLQGQIRVFLA
                     ERSIPPGDRWDREIAAAQRASRATAVLIAQDADDSWYLGDEIVTAIELHRAAPDTHRI
                     IPVLLAQGVSVPYGLSHVQVLDASGDTLPDVAHRLRAIVASPSSA"
     gene            complement(3504..6275)
                     /locus_tag="VF516_08080"
     CDS             complement(3504..6275)
                     /locus_tag="VF516_08080"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HEX8107673.1"
                     /translation="MFLKTAIPDSGREIAQYREWLQPAFNAADFGRLYSDLFGHPIAH
                     LVHDRTPLTDLIHETLLDCARQGRPLVQLLARAVRVRASRPQLAGPLAQALGLASLVE
                     VADLLVHDGCPDALRDEVRHAFAETTGARLASYHHDGRAGWIDVLVGLEELPALAGRR
                     PAVIEFLERIADAWHRADPSAASALARWLDQQRTPRGIAAAVDTAAVTTDPVSAWLAL
                     GLERHGRVKIVGFREAEISLPLDRVFVPLYMYADRRRRGAGPDRRADQDGLSDGGADI
                     SLDDAVARSGDDRPCLALVGDPGAGKTTLLRYLFRRVARGEVTIRVAHLRGLHPVLVR
                     LATVRDDEQVPRGLRAIVARIAAEDGHPEAGPALLARPGQRLLFLLDGLDEVRDEPAR
                     ERLCAWLNDEIDHWPGCGFIATSRLAAWARTPTLGARFLPVWVRGMRGTVRDDYVRRW
                     FGAVVHHFHGAVDSPAELEARANAKATALLDVLASGDWRSHPQLLEMVANPLMLSTLC
                     LAHYLRHPATRAAGRAVRAHPRPPDRRVDPPAQGWPGAPPRDRAPGAAAARLRDARAG
                     PPRAGQRGGRGARPGAARARPRVARGGADGGALPRPGARRVRRADEPRPGPGRVRASV
                     VPGVPRRLPRRRPRARGRAGRPRRRPALGGGHPARDVAARRVPAVHEPGARSRRRRHS
                     AAAPVLESVEQEQKALVRARQDRPARLGIAILRGGPRDDRPEAAWGELGVRDRGQPDE
                     HGVQAAQVGHRTSDVVKNDAAKQMAEQRGLARTRIADQRQAGPAIAGPRQRLVERDLG
                     TAVTEGPLVAAGVRVRPIASPPRIRMDLQRHEDVVELDRDCGVAEPDDRHAPVLRQPE
                     SPPLGDRFIRRHHRRRTQRSPSGAITSRSRILPRESTRPRSQPAPGPPAPTLQKTAYA
                     CAARRCRPR"
     gene            complement(6499..>7210)
                     /locus_tag="VF516_08085"
     CDS             complement(6499..>7210)
                     /locus_tag="VF516_08085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8107674.1"
                     /translation="RTRAIVLNNPSNPTGAVYSRAQVEALAEVCVEKDLLVVSDDIYR
                     QLVYGDAAYHSIAAVGPEIARRTILVDGASKTYAMTGWRIGYTAGPLPLIKAMAKIQG
                     QSTSNPTHIAQIAALAALTGPQDCVAEMRAAFNERRLAMVKLLRAIPGVSCREPKGAF
                     YAFPDVSSYLGKKSPESHILEDDVQLCDWLVEVGKVAVVPGSGFGAPGFVRLSYACSM
                     QDIEVGVGRMARALATLS"
BASE COUNT         1040 a         2496 c         2678 g          996 t
ORIGIN      
        1 tcgtcgagca gcacggtgtc ggtcagcgtg gtcgcgctgg tgttctccat gccgccgaag
       61 atgaagtcgg cgacgaacac ctgggcgtag cgcgggtagg ggtagggcac gccgaaccgc
      121 tcggagaaca gctccatcat ctgcggggtg cgggcgagcg tgcgctcggc ggcggcctcg
      181 cggccgcgct cgacgtagta caccaccggc acgtcgcgcc accgggtctc gatcgtggcg
      241 aagtcgccga ccgcgagcgt gatcaggtaa cagctgtgcg ggacgtcgag ccgccagtgc
      301 agggtgcggc gatcgccctc ggtgcggtcg gacaccagcg cgccgttgga caccgcgaac
      361 aggtgggccg gcacggtgac ggtgagctcg ctggtcgcct tctcgtgcgg cgcgtcgaag
      421 cacgggaacc agtaccgcga gtcctcgtcc tggccctgcg accagacctg gaccggcttg
      481 ccggggtagg cgtcgtcggg cgcaatgaag tacaggccgc ggcggggcgc gccgcggtag
      541 gcgacggtga tcgcgagctc ggcgccggcc gcggcgccct gaggggggac gggcagctcg
      601 atccgcagct tccggccgtc gtggcggaac gccgccggct cgccgccgac cgtgacgcgc
      661 tcgatggtca gctcgacggc gtcgagctcg acgctgcggg tgtcgtcggc gagcgcgcgg
      721 cagcgcaggg tcgcggtgcc ggcgacgctc ctcgccgcgg ggtcgacgtc gagcacgagc
      781 gagaggtgct ggatgtcgac gacgcggtcg ggcgcccagc gcgggcgggt tccggggaag
      841 gcaaactcgc ggatcatggc acagcgacca taccggatcg atcgcaccgt gcggtgatac
      901 aacgcctggc agccgacttc gagagcgacc acggcgcgcc cggcttcgag cgcagcaagg
      961 gcttcgcaga gctcgtgtgc agcacccgcg gcgtcacctc gccgatcact cgctggcgag
     1021 tacctcagcg atggtcgcag agatcgccgc gcgggcgatc cagcgatcga gtcgccgtgg
     1081 atcgcgttcg cggaggatcc gctcgcgcgc ggcgctgtcc ggatcgatgt ggcgggcggc
     1141 aaggatcgcg atcaaggcct cgatcttgcc ctcgatcttg ccttccgcct tgccggcagc
     1201 tcgatcctcc gcccggaccg cctcgatgac gaggttgtgc ctggccacca gggcgcgggc
     1261 gaccgcatcg tcggccttgg cggcgtggac catggcctcg atgggcagcg gcacctcgag
     1321 cgcgggatct gcaatatggc ccgcggcgtc gagctcgtgc caggtgccgc gatcggtcga
     1381 ccactccagg acgcgcgatc gctcgacgtc gatcgcgaac acccggcgca cgccgcgcgc
     1441 ggtgagcttg accgccttgg tggtggcgcg gccgagcgac tgggcgctga tgatctcgaa
     1501 cgcgagctgc tcgagctggc ggccgcccgt cctgggatcc ggggcagcgg ggtacacgct
     1561 gacatcgggc gcgatgtcat cgaccttgga ggtccgggtc agcagatcgg ccgcggcctc
     1621 gaactcgaac ccggtgtggg cctcgatcag cgcggcgagc tgcaggtggc gctcgccgtg
     1681 gggtggatcg gcgggggaca cgtagactag ctccccgtcg tacatctcgt agcgagtctc
     1741 gggctcgagg agacgatcgt cgacgcccga caggtgggag tcaacgtgcg aggcagcggg
     1801 cggcatgtcc cagcatacct ccgacgatgc acagcgcgga gcaggttctg cggagcgtac
     1861 ggcgatatca cgcgcggctt gagagcaagc gctgcgccgc ggccaacgcg gcgagatcac
     1921 agtcgagact gtccggggaa tggccgggat tcggacgctt gtccgagatc cggccggatc
     1981 ccggacaacc gacaaccgac aatcgacaac cgccatgcgc gcaagcgcgg tcggccgcta
     2041 cgtgccgctg cgagtccagc gtgctcgctc gtcgagcaac gcggcaaggc tgcggcacag
     2101 cgcctggttg ccggcggcga gctgtgcgac gtggagaatg cgcgtggcga ggggcgccgt
     2161 cgccggcgcg atgtgcccac gcggggcagg ggcgaggagc aggatctctt cgaagaccgt
     2221 gtcggtgagc tgcgtcagac gtgcgtgcag gcgccgatga tcacagtcgc caccggttgc
     2281 ggcgtcggcg caaccggcag cggctccagc gttgggtggc tcgttcatgc gctgctcgga
     2341 ctcgcgacga tcgcgcgcag tcggtgagcc acgtcgggaa gcgtgtcccc ggaggcgtcg
     2401 aggacctgga catgagatag cccgtacggc acggaaacgc cctgtgcaag caagacaggg
     2461 atgatccgat gcgtgtcggg agctgcgcga tgtagctcga tggcggtaac gatctcgtcg
     2521 ccgaggtacc aggagtcgtc cgcgtcctgg gcgatgagga cggccgtcgc ccgcgatgcg
     2581 cgctgggcgg cggcgatctc gcggtcccag cggtcgcccg gcggtatcga gcgctcggcg
     2641 aggaacacgc ggatctggcc ctggagcagc gagtacagcg catgggcgct gggcctgcct
     2701 ccgggcgagt gcgcgaggaa gaggtcatag cgcgcgctgg atgggatcga ggctggcctg
     2761 atctgctggc cgggcgcgcg gttccccgaa gcgccggccg gcggggcgtc gcctcgcttc
     2821 ccctcccgcg gtccgcgatg gtcgtcgagc cagcgggcga tctcggcggg gatgccatcg
     2881 tggctcgtcg accacgcggt cgtgagccgc tcgagatagt cgagcagcgg cggcggccgg
     2941 ccagccgctg ccgggagctc ctcgagccac accagcgcgt cggtccaggc ggcacgaccg
     3001 ctgtgaccat ggtcgaggta ggacgcgtct cggtccatcg cgagcgcctg agcaatctcg
     3061 tcgcacaacg tctcggggca gccatggtgc gcgaggagcc cggcgatctc gatcaccggc
     3121 gcgagcccca gcaccgcggc cagttgctcg acgatctcct tgcgatcggc gcgggcgcgc
     3181 acggcgcgtg cgaagagctg catcagcggg cgtcctcgct gggcgcagct ctcgagggtt
     3241 ctgtgaagca cgtcctggaa gctcccggtc acatcgacgt cgtgctggag cggccgcccg
     3301 aacaggtctg cgtagaggcg cgccaggctc gcgccgtcca gcgcagacga tagccatgcg
     3361 cggtactgcg cgagcgcccg accgccgtcg ggaacggtgt ccttcaggaa cacgcgcgcc
     3421 tcgccatcgc tgcgcggcac caccgtggcc ggtgccagcc ccatgcgccc atgccttcac
     3481 gttcggggat ccgggcggcc gagtcaacga ggtcggcatc tccgggccgc acaggcgtac
     3541 gctgttttct gcagcgtcgg cgcgggcggc cccggcgccg gctgggaccg gggccgtgtt
     3601 gactcgcgcg ggaggatcct cgatcgtgag gtgatggccc cggatgggga gcgctgcgtg
     3661 cggcgacgat ggtgacggcg gatgaaccgg tcgccgagtg gcggcgattc gggctggcgc
     3721 agcacgggcg cgtgacgatc gtcgggttcc gcgacgccgc aatcccgatc gagctcgacc
     3781 acatcttcgt gccgctgcag gtccatgcgg atccggggcg gcgaggcgat gggcctgacg
     3841 cgcaccccgg cggcgacaag ggggccctcg gtcacggcgg tgccgaggtc tcgctcgacg
     3901 aggcgctggc gcgggccagc gatggccgga cctgcctggc gctgatcggc gatccgggtg
     3961 cgggcaagac cacgctgctc cgccatctgt ttcgccgcgt cgttcttgac gacgtcgctg
     4021 gtccggtggc ccacctgcgc ggcctgcacc ccgtgctcgt ccggctggcc acggtcgcgg
     4081 acgccgagct cgccccacgc ggcctccggt cggtcatcgc gcgggccgcc gcggaggatg
     4141 gctatcccga ggcgggccgg gcggtcctgg cgcgcccgaa ccagggcttt ctgttcctgc
     4201 tcgacggact cgagcactgg cgcagcagcg ctgtgtcgac gtcgcctcga tcgagcgccc
     4261 ggctcgtgaa cggccggaac acgccgggcc gcgacatcgc gagcaggatg acctcctccc
     4321 agcgcgggtc gccggcgcgg tcggccagcg cggccccgag ctcgtggccg gcgacgtggc
     4381 aggcggcgag gtactcctgg aacgacagat gcacgaactc gacccggccc aggtcgcggc
     4441 tcgtcagcac gccgcactcg tcgcgcacca ggtcgaggaa gcgctccgcc gtcggcgcca
     4501 ccgcgcgcaa cgcggggacg cgcgcgagcg gcgcccggac gagctccacg gcctcctcgc
     4561 tggccagctc gcggcggtcc tgctcgtgca tcgcgtaggc gagcggctgc agcaccaggc
     4621 gcgcggtctc gaggcggagc acctggccat ccttgcgctg gcgggtccac acgtcgatca
     4681 ggaggccgag ggtgcgctcg tacagctcgc cccgctgctc gggtagccgg gtgtcgtaaa
     4741 tagtgtgcca ggcacagcgt ggacagcatc agcgggttgg ccaccatctc gagcagctgg
     4801 gggtggctgc gccagtctcc gctggccagc acgtcgagca gcgcggtggc cttcgcgttc
     4861 gcgcgggcct cgagctccgc gggcgagtcc accgcgccgt ggaagtgatg caccaccgcg
     4921 ccgaaccagc gccggacata gtcgtcgcgc acggtgccgc gcatcccccg cacccagacc
     4981 gggaggaacc tcgcgcccag cgtcggggtg cgcgcccatg ccgccaggcg actggtcgcg
     5041 atgaacccgc agcccggcca gtgatcgatc tcgtcgttga gccaggcgca cagccgctcg
     5101 cgggccggct cgtcgcggac ctcatcgagc ccatcgagca ggaacagcag gcgctggccg
     5161 gggcgggcga gcagcgccgg accggcctcg gggtgcccat cctctgccgc gatgcgcgcg
     5221 acgatcgcgc ggagcccgcg cggtacttgc tcgtcgtctc tcaccgtggc caggcgcacc
     5281 agcaccgggt gcaagccgcg caggtgcgcc acccggatag tgacctcgcc acgggccacg
     5341 cggcggaaca ggtagcggag cagcgtcgtc tttccggcgc ctggatcgcc gaccagcgcc
     5401 aggcacggcc gatcgtcgcc ggaccgggcg accgcgtcgt cgagcgagat gtcggccccg
     5461 ccatcgctga gaccatcctg gtcagcgcgc cggtcgggcc cggcgccccg acgcctccgg
     5521 tcggcgtaca tgtacagcgg cacgaacacg cgatcgagcg ggagcgagat ctcggcctcg
     5581 cgaaagccca cgatcttgac ccggccatgc cgctcgagac cgagcgcgag ccaggcggag
     5641 acggggtcgg tcgtgaccgc ggcggtgtcg acagcggcgg cgatgccccg cggtgtccgc
     5701 tgctggtcga gccagcgtgc gagcgcggaa gccgcactgg ggtcggcgcg atgccacgcg
     5761 tccgcgatgc gctcgaggaa ctcgatcacc gccggacggc ggccagccag cgccggaagc
     5821 tcctcgagcc cgaccaggac gtcgatccag ccggcgcgcc cgtcgtggtg atagctcgcg
     5881 agtcgcgcgc cggtcgtctc ggcgaacgca tggcggactt cgtcgcgcag cgcatcgggg
     5941 cagccatcgt gaacgagcag gtcggcgacc tcgaccagcg acgccagacc cagcgcctgg
     6001 gccagcgggc ccgcgagctg gggccggctc gcccgcacgc ggaccgcgcg cgcgagcagc
     6061 tgcacgagcg ggcgcccctg ccgcgcgcag tccaacaggg tctcgtggat cagatccgtg
     6121 agaggcgtcc tatcgtggac gaggtgcgcg atcgggtgcc cgaacagatc cgagtacagt
     6181 cgcccaaaat ccgccgcgtt gaacgcaggc tggagccact cgcgatactg cgcgatctcc
     6241 cgcccgctgt ccgggatcgc agtcttcagg aacatgcgcc gtgctcccgc gtcggtgcgt
     6301 cacaccggct tgctcggccc ccaccacatg tgtccatggc atcacgatcg gggatccacc
     6361 cgcgcccgtc aatgcagcgg ccggcagcgc tcctcgagca gcaaccggac gcctgcgtgg
     6421 tcgagcgcct gccgctgccg ccgagctctc gggtcacgca gtctggggca gcgacgtgca
     6481 ggccaccgag cggcgcgctc aggacagcgt cgcgagcgcc ctggccatcc ggccgacacc
     6541 gacctcgatg tcctgcatcg agcacgcgta cgacagccgc acgaagccgg gtgcgccgaa
     6601 ccccgagccg ggcacgaccg cgaccttgcc gacctcgacc agccagtcgc acagctggac
     6661 gtcgtcctcg aggatgtggc tctcgggcga cttcttgccc aggtaggacg acacgtcggg
     6721 gaatgcgtag aatgcgccct tgggctcgcg gcacgagacg ccggggatcg cgcgcagcag
     6781 cttgaccatc gcgagccggc gctcgttgaa cgccgcgcgc atctcggcga cgcagtcctg
     6841 cgggccggtc agcgcggcga gcgcggcgat ctgcgcgatg tgcgtcgggt tggacgtcga
     6901 ctggccctgg atcttggcca tcgccttgat cagcggcagc gggcccgcgg tgtagccgat
     6961 ccgccagccg gtcatcgcgt aggtcttcga cgcaccgtcg accaggatcg tgcgcctggc
     7021 gatctcgggg ccgaccgcgg cgatcgagtg gtacgccgcg tcgccgtaca ccagctggcg
     7081 atagatgtcg tcggacacca ccagcaggtc cttctcgacg cagacctcgg cgagcgcctc
     7141 gacctgggcg cgcgagtaca ccgcgccggt cgggttcgat gggttgttga gcacgatcgc
     7201 gcgggtccgg
//
LOCUS       DASVOR010000400         5239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266525,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000400 DASVOR010000000
VERSION     DASVOR010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5239
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266525"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VF516_08090"
     CDS             <1..376
                     /locus_tag="VF516_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107675.1"
                     /translation="VHTAEGDAAAPLRARSKVVPLVVGGAAVALAGGALGFELWARST
                     NSQSLDEKTSNDRRISLWHSANNRRYVAEGLGVASLAAAGVAVWLTLRPGAEALDGHT
                     ARVDLAPMVARQTAGLVAVGGF"
     gene            720..1610
                     /locus_tag="VF516_08095"
     CDS             720..1610
                     /locus_tag="VF516_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107676.1"
                     /translation="MQLDLSPATPVQADVRATPGPGELPAAMHFTLYAIQEETLPDMT
                     RQDHLFEVVRFEVHHIVDTTSPCFIDVGDHVPYPGLHVTQFAKRVEQDTMIPDPAHPP
                     AGATPQQLELVTTALARMAKIAAITGSGGLVAVTSASTTPYPAVAADCNGPSDQIPPP
                     QCIDDASSQRRLQLCQQTWKQDPHLWEGTDRVLTAPLNGTTFGLVLNPMTPGIGAPVG
                     GAQIFVDNALVGVDAYAIYFQADTPPPGMDGPGTQLYFGRPTMPTRGVFHVHLVSPIN
                     QALTAEMAVFADLGQDDVHF"
     gene            1619..2707
                     /locus_tag="VF516_08100"
     CDS             1619..2707
                     /locus_tag="VF516_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107677.1"
                     /translation="MYPRSVLLALLAALTPSCKGIDCGEGTTERNGSCVASGQTVSAA
                     RCGPFTVLQGDLCVPMFPPTVCDPGTTAEDTDKGTGVTTCIGTGAGGCAARLACPTPT
                     DGTQTICGQIFDFETGQAFAQSGATGAQCSAANPSGPCSLGIHAFDAVALAMNPASTP
                     PLTTGAVYIDDCGRYRIPGIAQPGGPFIALAIDDAAPAAMGPSGTTNATGVATAKMPS
                     FATKDLDAYVVKGATAAGWGMPPLANGIYAQVFHSKRTGPELASGVMTTFGPSNAPPP
                     TMVNKSRTFYFGASAVMRTTLDTAASATGMNGTALISGANLAELYGGTGGIPATCAWE
                     IHAGAAIPGVVFIQSYRPTDALGMTCPL"
     gene            2722..4644
                     /locus_tag="VF516_08105"
     CDS             2722..4644
                     /locus_tag="VF516_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8107678.1"
                     /translation="MTVLAVLAARPASADPTSGVDGALFRGAYDAGGVFSLEGARLLP
                     RRDLSFTLLVSYARAPLTLAVPGIGDAGSDRILNYLVTVDMAFGMSLTDRIAIGIDAA
                     GYRTATGTGYGVRGRYAGMGQIAQPSTGLISLRPLSNVDPSAPSGSAGYLGDELAGPL
                     DARFGLKVALAQRPLWALTAVGSVVLPFGDDQMLLGDANLVFEPRLAFEWRRDRVHAT
                     RLIVNLGARIRERTVLQVYDPMTTGQTPADAKAVLDVGSELVSGIGGVYELSPRLSAA
                     GELVSLAPLPDGLSWGDCRLYSGARCTALTSSNYFAGAHHGDLAVQVTGGVMLRVTPD
                     VTANLMVGTGLTGARGDQVRVTTGIVWSPQPGGGMATGRADRDGDGIPDAIDQCPDEP
                     EDKDGFQDEDGCPDPDNDKDGIPDAVDRCPNEPEDKDGFQDEDGCPDPDNDKDGIPDA
                     LDKCPDEPEDKDGFQDEDGCPDDDNDGDGFPDAVDKCPNDPETVNGFEDEDGCPDVRG
                     TTGPEERADRIDLKGAQVTFARGALTAPARQLLGQVATLIKNRRLAIRIEVHTALGTR
                     STSPAQIAAQRRRDKTLAQQRAKLIADYLVSQGVPAPQLQAVGIGSDRPLGTATPSDP
                     VNERVDFIKAQQGGSP"
     gene            4641..>5239
                     /locus_tag="VF516_08110"
     CDS             4641..>5239
                     /locus_tag="VF516_08110"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8107679.1"
                     /translation="MTAQRSAGVALIALALAGGPALAQPGGSTPTDVEVPAVVLEPQN
                     ESQGSGADEALDLANIVQSAAKGVTTVQEAPAIVTVVTADEIRDRQFQDLAQLADSVP
                     GWQRASSYHSTFPGTLVRGQIQAVQFLHDGVSLFDPFINVASVSRVQPMELIKRVEMI
                     TGPGGVLWGSNSLLGILNVITKDAEDVDGVEVGGQVGDGG"
BASE COUNT          762 a         1909 c         1887 g          681 t
ORIGIN      
        1 ggtccacacg gcggagggcg atgcggcggc gcccctgcgt gcgcgctcga aggtcgttcc
       61 gctggtggtc ggcggcgccg cggtcgcgct ggccggcggg gcgctcggct tcgagctgtg
      121 ggcgcgctcg acgaactcgc agagcctcga cgaaaagacc agcaatgacc ggcggatctc
      181 gctgtggcat tcggccaaca acaggcgcta cgtggccgag ggcctcggcg tggccagcct
      241 ggccgcggcc ggggtcgccg tctggctcac cctgcgccct ggcgccgagg cgctggatgg
      301 ccacacggcg cgcgtcgatc tcgcgccgat ggtcgcgcgc cagaccgccg ggctggtcgc
      361 cgtcggcggc ttctgatcga tccgcggtcg cccacctcgg ccggctcgcc ggccccgtca
      421 ccggccgtgc gatccgccac gcgggtgtcg cgattgccga gcgttgccgg cgagatcccc
      481 ccgatccgtc ggggtggttc gccccggggt gcccgcccgg ggtgcccgcg gcacgctcgc
      541 gtgcgccggc gggacagccg tggatcgcgc gcgccaacta gtagcttttg aggttcggcg
      601 cgatctacca tttttcttcg caagggggac cgcgcgacgt gcgtcgagct acacgaggaa
      661 cagttgccgc cgcagcgctg gcgctggccg gctgtgtcaa cggatttcag ggcgcgaacg
      721 tgcagctgga tctgtcaccg gccacgccgg tccaggccga cgtgcgcgcg acgcccggcc
      781 cgggcgagct gcccgcggcg atgcacttca cgctgtacgc gatccaggag gagacgctgc
      841 cggacatgac caggcaggat cacctgttcg aggtcgtccg gttcgaggtc catcatatcg
      901 tcgacaccac gtcgccgtgc ttcatcgacg tcggcgacca tgtgccgtac ccggggctgc
      961 acgtcacgca gttcgccaag cgggtcgagc aggacaccat gatccccgat cccgcgcacc
     1021 cgccggccgg ggcgaccccg cagcagctcg agctggtgac gacggcgctg gcgcggatgg
     1081 cgaagatcgc tgcgatcacc ggctccggag gcctggtcgc ggtgacctcg gcctcgacca
     1141 cgccgtatcc ggcggtggcg gccgactgca acggcccctc ggaccagatc ccgccgccgc
     1201 agtgcatcga cgacgcgtcc agccagcgcc ggctccagct gtgccagcag acctggaagc
     1261 aggatccgca cctgtgggag ggcaccgacc gcgtgctcac cgcgccgctc aacggcacga
     1321 ccttcggcct ggtgctgaac ccgatgaccc cgggcatcgg ggcgccggtc ggcggcgcgc
     1381 agatcttcgt cgacaacgcg ctggtcgggg tcgacgcgta cgcgatctac ttccaggccg
     1441 ataccccgcc gccgggcatg gacggaccgg gcacgcagct gtacttcggc cggccgacca
     1501 tgccgacccg cggcgtgttt cacgtccacc tggtcagccc gatcaaccag gcgctcacgg
     1561 ccgagatggc ggtgttcgcc gacctcggcc aggacgacgt ccacttctag gagcgcgcat
     1621 gtatccacgg agcgtccttc tcgcgctgct ggcggctctc accccgagct gcaagggcat
     1681 cgattgcggc gagggcacga ccgagcgcaa cggcagctgc gttgcctcgg gccagaccgt
     1741 gagcgccgcc aggtgcggcc cgttcacggt gctgcaaggc gacctgtgcg tcccgatgtt
     1801 cccgccgacg gtgtgcgatc cgggcacgac ggcggaggac accgacaagg gcacgggggt
     1861 gacgacgtgc atcgggaccg gcgcgggcgg ctgcgcggcc cggctcgcct gcccgacgcc
     1921 gaccgacggc acgcagacga tctgcggcca gatcttcgac ttcgagaccg gccaggcgtt
     1981 cgcgcagtcg ggagccaccg gcgcgcagtg cagcgcggcg aacccgagcg ggccgtgctc
     2041 gctggggatc cacgcgttcg acgcggtggc gctcgcgatg aacccggcga gcacgccacc
     2101 gctgaccacc ggagcggtct acatcgacga ttgcggtcgc taccggatcc cggggatcgc
     2161 tcagcccggc gggccgttca tcgcgctcgc gatcgacgac gcggcgccgg ccgcgatggg
     2221 cccctccggg accaccaacg cgaccggggt cgccacggcg aagatgccca gcttcgcgac
     2281 caaggacctc gacgcctacg tggtcaaggg cgcgacggcg gcggggtggg gcatgccgcc
     2341 gctcgccaac gggatctacg cgcaggtctt tcacagcaag cgcaccggcc cggagctcgc
     2401 gagcggcgtg atgaccacgt tcggaccgtc caacgcgccg ccccccacga tggtcaacaa
     2461 gagccgtacg ttttacttcg gggcgtccgc cgtcatgcgg acaacgctcg acaccgccgc
     2521 cagtgcgacc gggatgaacg gcaccgcgct gatcagcgga gcaaacctcg ccgagctgta
     2581 cggagggacg ggcggcatcc cggcgacgtg tgcgtgggag atccacgcgg gcgccgcgat
     2641 ccccggcgtc gtgttcatcc agagctatcg gcccactgac gcgctcggca tgacgtgtcc
     2701 cctctgaagc accttcctct cgtgaccgtg ctcgccgtgc tcgcggcgcg ccccgcgtcg
     2761 gccgatccga cgagcggcgt cgacggcgcg ctgttccgcg gcgcgtacga cgccggcggc
     2821 gtgttcagcc tcgagggcgc gcggctgttg ccccggcgcg acctgtcgtt cacgctgctg
     2881 gtgagctacg cgcgcgcgcc gctgacgctc gccgtgcccg ggatcggcga cgccggcagc
     2941 gaccggatcc tcaactacct ggtcacggtc gacatggcgt tcggcatgtc gctgaccgac
     3001 cggatcgcga tcgggatcga cgccgccggc tatcgcaccg cgaccggcac cggctacggg
     3061 gtgcgcggcc gctacgccgg catggggcag atcgcgcagc cgtcgaccgg cctgatctcg
     3121 ctgcggccgc tgtcgaacgt cgacccgtcg gcgccgtcgg gcagcgcggg ctacctcggc
     3181 gacgagctcg ccggcccgct cgacgcccgg ttcgggctca aggtcgcgct ggcccagcgc
     3241 ccgctgtggg cgctgaccgc cgtcggctcg gtggtcctgc cgttcggcga cgaccagatg
     3301 ctgctcggcg acgccaacct ggtgttcgag ccccggctcg cgttcgagtg gcggcgcgac
     3361 cgggtccacg cgacgcggct gatcgtcaac ctcggcgccc ggatccgcga gcgcaccgtg
     3421 ctgcaggtct acgacccgat gacgaccggc cagaccccgg ccgacgccaa ggcggtgctc
     3481 gatgtcggca gcgagctggt gtccgggatc ggcggcgtgt acgagctgtc cccgcggctg
     3541 tcggcggccg gcgagctggt ctcgctcgcc ccgctgcccg acgggctgtc gtggggtgat
     3601 tgccggctgt acagcggcgc gcgctgcacc gcgctcacgt cgagcaacta cttcgccggc
     3661 gcccaccacg gcgacctcgc ggtccaggtc accggcggcg tgatgctgcg ggtgaccccc
     3721 gacgtcaccg ccaacctgat ggtcgggacc ggcctgaccg gtgcgcgcgg cgatcaggtc
     3781 cgggtcacga ccggcatcgt gtggtcgccg cagcccggcg gcggcatggc gaccggccgc
     3841 gcggaccgcg acggcgacgg catccccgac gcgatcgacc agtgtccgga cgagccggag
     3901 gacaaggacg ggttccagga cgaggacggc tgccccgatc cggacaacga caaggacggc
     3961 atccccgatg cggtcgaccg gtgcccgaac gagccggagg acaaggacgg gttccaggac
     4021 gaggacggct gccccgatcc ggacaacgac aaggacggca tccccgacgc gctcgacaag
     4081 tgccccgacg agccggagga caaggacggg ttccaggacg aggacggctg ccccgacgac
     4141 gacaacgacg gcgacgggtt ccccgacgcg gtcgacaagt gcccgaacga ccccgagacc
     4201 gtcaacggct tcgaggacga ggacggctgc cccgacgtcc gcggcacgac cggccccgag
     4261 gagcgcgccg accggatcga cctcaagggc gcgcaggtga cgttcgcgcg cggcgcgctg
     4321 accgcgccgg cccgccagct gctcggccag gtcgcgacgc tgatcaagaa ccggcggctc
     4381 gcgatccgga tcgaggtcca caccgcgctc ggcacccggt cgaccagccc cgcgcagatc
     4441 gcggcgcagc gccggcgcga caagacgctc gcccagcagc gcgccaagct gatcgccgac
     4501 tacctggtga gccagggcgt cccggcgccg cagctccagg cggtcggcat cggctcggac
     4561 cgcccgctgg gcaccgcgac gccgagcgat ccggtcaacg agcgggtcga cttcatcaag
     4621 gcgcagcaag ggggatcgcc atgaccgcgc agcgcagcgc aggggtggcg ctgatcgcgc
     4681 tcgcgctcgc gggcggtccg gcgctcgcac aacccggggg cagtacgccg accgacgtcg
     4741 aggtccccgc ggtggtgctc gagccgcaga acgagagcca ggggtcgggg gccgacgagg
     4801 cgctcgacct cgccaacatc gtgcagagcg ccgccaaggg cgtgaccacg gtgcaggagg
     4861 cgccggcgat cgtcaccgtg gtcaccgccg acgagatccg cgatcgccag ttccaggacc
     4921 tggcacagct cgccgactcc gtgccgggct ggcagcgcgc gagctcctac cactcgacgt
     4981 tccccggcac gctggtgcgc ggccagatcc aggccgtgca gttcctgcac gacggcgtgt
     5041 cgctgttcga cccgttcatc aacgtggcga gcgtcagccg ggtccagccg atggagctga
     5101 tcaagcgggt cgagatgatc accggcccgg gcggcgtgct gtggggctcg aactcgctgc
     5161 tcggcatcct caacgtgatc accaaggacg ccgaggacgt cgacggcgtc gaggtcggcg
     5221 gccaggtcgg cgacggcgg
//
LOCUS       DASVOR010000401         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_442057,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000401 DASVOR010000000
VERSION     DASVOR010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442057"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF516_08115"
     CDS             complement(<1..248)
                     /locus_tag="VF516_08115"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX8107680.1"
                     /translation="MTASDDHAPQPGASVSIAALGLVVFTDENGAYLLPVPPGPHVVR
                     IEAAGYLPVDKTVTVASTPATLDVQVDDRGSSEVITLIG"
     gene            complement(711..>1675)
                     /locus_tag="VF516_08120"
     CDS             complement(711..>1675)
                     /locus_tag="VF516_08120"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107681.1"
                     /translation="KAALFLAGDRRYDKDLRAVDTSPIANERINSWSFYSDRRRRGLE
                     LSVFFDLFGNDPAGEPLATRVAEGLVGQVPYYFNTQELVWGVTGLGKWVTGLGAKGTA
                     GGTLTADGVTIAPRAPRHTSNDKAWAVRRASEYKQLTLDVPAAAAGTWLVISSEGVRP
                     GSDYKVGGNGLSVARTYRGLDGSALDLTAGQVKLGDLVYVELELTNTSGAAIQNIALV
                     DRLPAGFEIENPRLGRSTKADWMKDEEMWTPDFMNLRDDRLEAFGALAARTGKKVIYT
                     VRAVTSGTFTVPPVEAGAMYDPTLWARGKGGSAVIGGPWTGKTI"
BASE COUNT          219 a          589 c          588 g          279 t
ORIGIN      
        1 ccgatgagcg tgatcacctc gctgctgccc ctgtcgtcga cctggacgtc gagcgtggcg
       61 ggcgtcgatg ccacggtcac ggtcttgtcg accggcaggt agcccgccgc ctcgatccgg
      121 accacgtgcg gtccgggcgg caccggcagc aagtacgcgc cgttctcgtc ggtgaacacg
      181 acgaggccga gcgcggcgat gctcacgctg gccccgggct gcggcgcgtg gtcgtcgctg
      241 gcggtcaccc tgccgtgcag cgtcgtggcc ggcgcggcgg gcgcagccac gtccgccggg
      301 ggcggagacg gctcggtcgc cggctccggg ctcggagcct gggccagcgc cggtgtcgct
      361 atccacagac tcgatagcac agttgtggtt gccagtgtgg atgacaccct cgtgaaggcc
      421 attcttgttg cgaccctagc accgtggtgt gcagccgctc ggcggcgcat cgtggcgatc
      481 tacggcagac cgccagcggc tcgtcatcgg gatacacggg agctgtgtca ctcacacgtt
      541 cgctgacacg ctgatcggca cgcgctacgt gagcatgagt gtagaaatgg attgtcgagg
      601 agcctgtgac acaggcgctc agcctggtcg agctcaccgg cgtggatcag ccgagtctgg
      661 caccgtttgc actccatgcc gaccgtcgag actctgcgtc atggcgcggg tcagatcgtc
      721 ttgccggtcc acggcccgcc gatcaccgcg ctgccgccct tgccgcgcgc ccacagcgtc
      781 gggtcgtaca tcgcgccggc ctcgaccggc gggacggtga acgtgcccga ggtcaccgcg
      841 cgcaccgtgt agatcacctt cttgccggtg cgcgccgcca gcgcgccgaa cgcctcgagc
      901 cggtcgtcgc gcaggttcat gaagtcgggc gtccacatct cctcgtcctt catccagtcg
      961 gccttggtcg accggccgag ccgtgggttc tcgatctcga agccggcggg cagccgatcg
     1021 accagcgcga tgttctggat cgctgcgccg ctggtgttgg tgagctcgag ctcgacgtac
     1081 accagatcgc cgagcttgac ctggccggcc gtcaggtcga gcgcgctgcc gtcgaggccg
     1141 cggtaggtcc gcgcgacgga caggccgttg ccgccgacct tgtagtcgct gccgggccgc
     1201 acgccctcgc tgctgatcac cagccacgtg ccggccgccg ccgcggggac gtcgagcgtg
     1261 agctgcttgt actcgctggc gcgccgcacc gcccacgcct tgtcgttgct ggtgtgcctc
     1321 ggcgcgcgcg gcgcgattgt caccccgtcg gcggtcagcg tgccgccggc ggtgcccttg
     1381 gcgcccagcc cggtgaccca cttgccgagg ccggtcacgc cccacaccag ctcctgggtg
     1441 ttgaagtagt acggcacctg gccgaccagg ccctccgcga cgcgggtcgc gagcggctcg
     1501 ccggccggat cgttgccgaa caggtcgaag aacacgctga gctcgaggcc gcggcgccgg
     1561 cggtcggagt agaagctcca gctgttgatc cgctcgtttg cgatcggcga ggtgtcgacc
     1621 gcgcgcaggt ccttgtcgta gcgccggtcg ccggcgagga acagcgcggc cttca
//
LOCUS       DASVOR010000402         2613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_91664,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000402 DASVOR010000000
VERSION     DASVOR010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91664"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="VF516_08125"
     CDS             <1..197
                     /locus_tag="VF516_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107682.1"
                     /translation="PVATPVQPPPTPLPSTQQPTDSKALSESDPIGLDFEAVDDAPEA
                     DDPEDLDAEPDAEADFDADA"
     gene            229..1029
                     /locus_tag="VF516_08130"
     CDS             229..1029
                     /locus_tag="VF516_08130"
                     /inference="COORDINATES: protein motif:HMM:NF018533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX8107683.1"
                     /translation="MPEGDSIHRIAALLAPRLTGRKLERVTTQGLVREAAGRTVTKVS
                     AHGKHLVIELDNGAYLRAHLGMNGRFRAYDRASGEAMLARLSPGRASLALVTADGVYV
                     WIGAPQIEVAQRRDPRRGMAVGALGPDVLAADFDPRTAAERAAEHGDRRIADVLLDQR
                     VVAGIGNIYKSEALFAAGVDPRTPVARLDPRRLETIYAAARRLMSSRLATRDPLIGSP
                     ASDRAVYGRTGKPCLRCGSTIACYSLGEPPRWTWSCPVCQPVTEMSRR"
     gene            1154..1705
                     /locus_tag="VF516_08135"
     CDS             1154..1705
                     /locus_tag="VF516_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107684.1"
                     /translation="MLQLSRTWLRKAGTAVAQRAGTSKGTSMVPAVAYATPIHPLSSE
                     VSTTLTVSAGVAYEIFSDALEIPRWLPIVQAARTLARYPDGRPERVAFTRKLERGSLG
                     YTLEYRYDPAHLTVAWTTPEASNVVLTGEARFVALSNRACLMLYRLVLDLPVVDDLLR
                     SELDHHPASHVVAEFREHLRRLC"
     gene            1849..>2613
                     /locus_tag="VF516_08140"
     CDS             1849..>2613
                     /locus_tag="VF516_08140"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8107685.1"
                     /translation="MSNERRQDRSPPSSSPALPLPPPPPHGQDADVEPVFATGSAAHA
                     LYEASEAAYHLRELEDKLQRCLATLTLHFQPIVHAGTRARFGYEALLRSADRSLPHPG
                     AILDAAERLERTATLGRNVRAQAAKVIGSTPPERGLMFVNLHLLDLFDKQLLSPFAPL
                     SKVASRVVLEITERTSLEGQIDLRYRVAELRELGFHIAIDDLGGGHARMGTFTPLDTD
                     FVKLDMSLVRDVDKHQLKQRLIRSVTQLCREQGTQVI"
BASE COUNT          392 a          935 c          888 g          398 t
ORIGIN      
        1 cgcccgtcgc aacgccggtg caacccccgc caactccgtt gccatcaact cagcaaccta
       61 ctgattcaaa agcactttca gaaagcgatc cgatcggcct cgacttcgag gcggtcgacg
      121 atgccccgga ggcggacgat ccggaggacc tcgacgccga gccggatgcc gaggccgact
      181 tcgacgccga tgcgtgaccg gcctcgttga aagattactc tcctcgccat gcccgagggc
      241 gacagcatcc accgcatcgc ggcattgctc gccccccggc tgaccggtcg gaagctggag
      301 cgggtgacca cgcaggggct ggttcgcgag gccgccgggc ggacggtgac gaaggtgtcg
      361 gcgcacggca agcacctcgt gatcgagctc gacaacggcg cctacctgcg cgcgcacctg
      421 gggatgaacg ggcggttccg ggcctacgac cgggcctccg gcgaggcgat gctggcgcgg
      481 ctgtcgcccg gccgggccag cctggcgctg gtcaccgccg acggcgtgta cgtgtggatc
      541 ggtgcgccgc agatcgaggt cgcccagcgc cgcgatcccc ggcgcggcat ggcggtgggg
      601 gcactggggc ccgacgtgct ggcggctgac ttcgatccgc ggaccgctgc cgagcgcgcc
      661 gccgagcacg gcgatcgccg gatcgccgac gtcctgctcg atcagcgcgt cgtcgccggg
      721 atcggcaaca tctacaagag cgaggcgctg ttcgccgccg gggtcgatcc gcgcacgccg
      781 gtcgcgcgcc tcgatccccg caggctggag acgatctacg cggcggcgcg ccggctgatg
      841 tcgtcgcggc tcgcgacgcg cgatccgctg atcgggtcgc cggcctcgga ccgtgcggtg
      901 tatggccgga ccggcaagcc gtgcctgcgg tgcggctcga cgatcgcgtg ctactcgctc
      961 ggcgagccgc cgcgctggac ctggtcgtgc ccggtttgcc agccggtgac cgagatgtca
     1021 cgtcgctagc ccagccgatc cccaccggga gcgccatcgc aacagacggt gtgcagatcc
     1081 gtacggcacg ttgcagaaat ggacacccca ggttccgtgg ctagtcgatc agccggctga
     1141 aacctcacga agcttgctgc aactgtccag aacctggttg cgcaaggctg gtaccgcggt
     1201 tgctcagcgt gcgggcactt cgaaaggaac gtccatggta ccggccgtcg cgtacgccac
     1261 gcccatccac ccgctgtcca gcgaagtctc gacgacgctg acggtgagcg ccggcgtcgc
     1321 ctacgagatc ttttcagatg cgttggaaat cccgcgctgg ctcccgatcg tccaggcggc
     1381 gcgcacgctc gcgcgctacc ccgacgggcg cccggagcgg gtcgcgttca cccgcaagct
     1441 ggagcgcggc tccctgggat acacgctcga gtaccgctac gatccggccc acctgaccgt
     1501 ggcgtggacg acgccggagg cgtctaacgt cgtgctcact ggcgaggcac ggttcgtggc
     1561 gctgtcgaac cgcgcgtgcc tgatgctgta ccggctcgta ctcgatctac cggtcgtcga
     1621 tgatctgctg cgcagcgagc tcgaccatca tccggcgtcg cacgtcgtcg cggagtttcg
     1681 cgagcatctg cggcggctgt gctgagcgat ctgcgcgcga ttcagtgacc gtgcgcctgg
     1741 cccgtgcgca agatcgtgcg cccacgcgcc ccacggtggc ctcacgcaac cacatgcggc
     1801 gggcgcgatg aatggtgacg gtggtcgaag ttgtgtacga tatttgtcgt gtcgaacgag
     1861 cgtcgtcagg atcggtcgcc accttcctcg tcgccagcgc tgccgctgcc gcccccgccg
     1921 ccgcacgggc aggatgccga cgtcgagccg gtgtttgcga ccgggagcgc cgcccacgcg
     1981 ctgtacgagg cgagcgaagc cgcgtaccac ctgcgcgagc tcgaggacaa gctgcagcgc
     2041 tgcctcgcca cgctgaccct gcacttccag ccgatcgtcc acgccgggac ccgggcccgg
     2101 ttcggctacg aggcgctgct gcgctcggcc gaccgctcgc tgccccaccc gggagcgatc
     2161 ctcgacgccg ccgagcggct ggagcggacc gcgaccctgg gccgcaacgt gcgcgcccag
     2221 gccgccaagg tgatcgggag cacgccgccc gagcgcggcc tgatgttcgt caacctccac
     2281 ctgctcgacc tgttcgacaa gcagctgctg tcgccgttcg cgccgctgtc caaggtcgcg
     2341 tcgcgcgtcg tcctcgagat caccgagcgg acgtcgctgg agggccagat cgacctgcgc
     2401 taccgggtcg ccgagctgcg cgagctcggc ttccacatcg cgatcgatga cctcggcggc
     2461 ggccacgcgc gaatgggcac gttcacgccg ctcgacaccg acttcgtcaa gctcgacatg
     2521 tcgctggtgc gcgacgtcga caagcaccag ctcaagcagc ggctgatccg ctcggtcacc
     2581 cagctgtgcc gcgagcaggg cacgcaggtg atc
//
LOCUS       DASVOR010000403         2975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_442101,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000403 DASVOR010000000
VERSION     DASVOR010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442101"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2939)
                     /locus_tag="VF516_08145"
     CDS             complement(<1..2939)
                     /locus_tag="VF516_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8107686.1"
                     /translation="MASWLVTAVETSRAHARRPDAVPAAPVAAGAHSTFDDAVAIVGT
                     GMRLPGGIASLGALWDVLVREQDTVREIPKDRFDVDLYYDADPDAEGTSYVRHASLLD
                     DVAGFDAAFFGISPREAAAMDPQQRILLELAWEALERAGIPPGDLRDSNTGVFVGAEP
                     GEYGRYRPSPATDAYVLTGSLPSFYAGRLSYHLGFQGPALAVDTACSSSLVAVHLACE
                     ALRAGRCDLALAAGSRVLADPEAFVALCRARALAPDGRSKAFSVRADGYGRGEGAVVL
                     VLRRLAEARAAGAPILGVIRGTAVNHDGASSGITAPNGTSQQKVLRAALRAARLSPAA
                     IDYIECHGTGTALGDPIEVQAIAAVYGQGRGAAGPIGLGTAKSTIGHLEAASGIAGIC
                     RILAALRHEELPATLHSSPRNPDIAWDELAVEVIDRRRPWPLRAGRPRRAGVSSFGLS
                     GTNAHLILEEAPAMASSSSAVQIEPAALATPPVLLSGRTEPALRAQTRRWAAWLADRE
                     PALVDLALSSERHRSHLEHRAAFVVDDVAALRDALDCFARGADHPAIVSGAAEPVEAR
                     PVFVYSGAGSQWRAMGRILLDTSPAFRARIAECDAVLAPLTGWSVTSVLRGDAPEELA
                     ELQHMDVVQPAIFAMNLGLTAAWRALGVEPAAVVGHSQGEVAAAVVAGALELGDAARV
                     ICARSAAIRRLARPGGMALLGRSAAEVETLIAGHGDALEIAVVNTPSSTVVSGDLDAV
                     DRLLAELAGRDIFARRIEVDYASHCAHMDPLLPGLARELACIRPRPAQIPLYSTVEGA
                     VVAGEQLDAAYWCRNLRQPVRLDRAMAAMLGAGHRVFVESSAHPILLEALRTTLADHR
                     GIAIGSLRRNAGGADQLVRGLAALHVGGSPVDWSKLHAAGTGRRIEIPTYAFQHETHW
                     LPPRKAGSDAGALGMTAVAHPIVGAATELPGGGRLLTGQLAATSPAWIADHVIHDAIV
                     LPGSGVA"
BASE COUNT          404 a         1084 c         1132 g          355 t
ORIGIN      
        1 tcggcgacac cgctgccggg cagcacgatc gcgtcgtgga tcacgtggtc ggcgatccag
       61 gccgggctgg tcgccgcgag ctggcctgtc agcagtcgac ctccgccggg gagctcggtc
      121 gcggcgccga cgatcgggtg cgcgaccgcc gtcatgccga gcgcgccggc gtcgctgccg
      181 gccttgcgcg gcggcagcca gtgcgtctcg tgctggaacg cgtacgtcgg gatctcgatc
      241 ctgcgcccgg tgccggccgc gtgaagcttc gaccagtcga ccggggagcc gccgacgtgc
      301 agcgccgcga ggccgcggac gagctggtcg gccccgccgg cgttccggcg cagcgagccg
      361 atcgcgatcc cgcggtgatc ggcgagggtc gtgcgcagcg cctcgagcag gatcgggtgg
      421 gcgctgctct cgacgaacac gcggtgtccc gcaccgagca tcgcggccat cgcgcggtcg
      481 agccgcaccg gctggcggag gtttcggcac cagtacgccg cgtcgagctg ctcgcccgcg
      541 accaccgcgc cctcgacggt cgaatacaac gggatctgcg cggggcgcgg ccggatgcac
      601 gcgagctcgc gcgcgagccc cggcagcagc ggatccatgt gcgcgcagtg cgacgcgtag
      661 tcgacctcga tccgccgcgc gaagatgtcc cggccggcaa gctcggcgag gagccggtcg
      721 accgcgtcga ggtcgccgga aaccaccgtc gagctcggcg tgttgacgac cgcgatctcg
      781 agtgcatcgc cgtggccggc gatcagcgtc tcgacctcgg ccgcggatcg cccgaggagc
      841 gccatcccgc cgggccgggc gagcctccgg atggcggcgc tgcgcgcaca gatgacgcgc
      901 gcggcatcgc cgagctcgag tgcgccagcg accaccgccg cggcgacctc gccctggctg
      961 tggccgacca cggcggcagg ctcgactccg agcgcgcgcc acgccgcggt caggccgagg
     1021 ttcatcgcga agatcgcggg ctggacgacg tccatgtgct ggagctcggc cagctcctcg
     1081 ggcgcgtcgc cgcggagcac cgaggtcacc gaccagccgg tcagcggcgc aagcaccgcg
     1141 tcgcactcgg cgatccgcgc gcggaacgcg gggctcgtgt cgaggaggat cctccccatc
     1201 gcgcgccact ggctgccggc gcccgagtac acgaagaccg gccgggcctc gaccggctcg
     1261 gccgcaccgg agacgatcgc ggggtgatcc gcgccgcgcg cgaagcagtc cagcgcgtcg
     1321 cgcagcgcgg cgacatcgtc gacgacgaac gccgcgcggt gctcgaggtg agagcggtgg
     1381 cgctcggagc tcagcgcgag atcgacgagc gcgggctcgc gatcggcgag ccaggcggcc
     1441 caccgccgag tctgcgcgcg cagcgctggc tcggtccgcc cggacagcag gaccggcggg
     1501 gtcgcgagcg cggcgggctc gatctggacg gcgctcgacg acgaggccat tgcgggcgcc
     1561 tcctcgagga tcaggtgcgc gttcgtgccg ctcaggccga acgacgagac gccggcgcgg
     1621 cgcggccgcc ccgcacgcaa cggccacggg cggcggcggt cgatcacctc gaccgcgagc
     1681 tcgtcccacg cgatgtccgg gttgcgcggg ctgctgtgaa gcgtggccgg cagctcctcg
     1741 tggcggagcg cggccaggat ccggcagatc ccggcgatgc ccgacgccgc ctcgaggtgg
     1801 ccgatcgtgc tctttgcggt acccaggccg atcggcccgg cggcaccgcg gccctggccg
     1861 tacacggcgg cgatcgcctg gacctcgatc ggatcaccga gcgcggtgcc ggtgccgtgg
     1921 cactcgatat aatcgatggc ggccggcgag agccgggccg cgcgcagggc cgcgcgcagg
     1981 accttctgct gcgaggtacc gttcggcgcg gtgatgccgc tgctcgcgcc gtcgtggttg
     2041 accgcggtgc cgcggatcac gccgaggatc ggcgcgccgg ccgcacgtgc ttcggcgagg
     2101 cggcggagca cgagcacgac cgcgccctcg ccgcgtccgt aaccgtccgc gcgcaccgag
     2161 aacgcctttg atctaccgtc gggcgcgagc gcgcgcgccc ggcacagcgc cacgaacgct
     2221 tccggatcgg cgaggacgcg cgatccagcc gccagggcga gatcgcagcg cccggcgcgg
     2281 agtgcctcgc acgccaggtg gaccgcgacc agcgacgagc tgcacgcggt gtcgaccgcg
     2341 agggccggcc cctggaaccc gaggtggtac gacagccggc cggcgtagaa gctcggcagc
     2401 gatccggtga ggacgtacgc gtccgtggcc gggctgggcc ggtaccgacc gtactcgccg
     2461 ggctccgcgc cgacgaacac gccggtgttc gagtcgcgca gatcaccggg ggggatcccg
     2521 gcgcgctcga gcgcctccca cgcgagctcg aggaggatcc gctgctgagg atccatcgcg
     2581 gcggcctcgc gcgggctgat cccgaagaac gcggcgtcga accccgccac gtcgtcgagc
     2641 agcgacgcgt ggcggacgta ggatgtgccc tcggcatcgg gatccgcgtc gtaatagaga
     2701 tcgacgtcga agcggtcctt cgggatctcg cggacggtgt cctgctcgcg gaccagcacg
     2761 tcccacagcg caccgagact ggcgatgccg cccggcagcc gcatacccgt gccgacgatc
     2821 gccaccgcgt catcgaaggt ggagtgcgcg ccggccgcga ccggtgcggc cggaactgcg
     2881 tcgggccggc gggcgtgcgc gcgcgatgtc tcgaccgccg tcacgagcca gctcgccacg
     2941 gcgtggacgt tcggctggtc gaacgcgagc gtgcg
//
LOCUS       DASVOR010000404         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412327,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000404 DASVOR010000000
VERSION     DASVOR010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412327"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..629
                     /locus_tag="VF516_08150"
     CDS             <1..629
                     /locus_tag="VF516_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107687.1"
                     /translation="TVEQAEARAMAAMDGCRAPWWVAGGWALELHAAAHGHRNRPHAD
                     LEISILRRDQRALFDHLAGWQRCAMVRPGVLEHWDGRTLPAEVHQIWARRGAPLSPDP
                     DRFAADPTLLDFLLEEADGDTWRFRRQPSIARPLAQLGAITARGVPFIRPEVALLYKA
                     KHLRFKDQRDFDAAAPALDEAARAWLATALDQAHPDHPWGLALDRAGA"
     gene            complement(632..811)
                     /locus_tag="VF516_08155"
     CDS             complement(632..811)
                     /locus_tag="VF516_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLD nuclease N-terminal domain-containing
                     protein"
                     /protein_id="HEX8107688.1"
                     /translation="MILLILSMSGVWLWMLLDCIRYENERDKVVWILVIVLAGIIGAL
                     IYLLARRVPRRRTSG"
     gene            complement(904..>1626)
                     /locus_tag="VF516_08160"
     CDS             complement(904..>1626)
                     /locus_tag="VF516_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107689.1"
                     /translation="RATLRGDSTTVASATPGTAADDSATREQLLARTREQQAQLIELR
                     AQLARLEPTAAPAATEDGADPDRPWHDPSPEQLAAWVKTCHLRFDMPDFEHGMPTPGS
                     GVTAEEVDGFHAAMAETGRRWQDLVHSLYVETTGDTAGADALSTGSMHREIVEKSSPA
                     EYNALLQRLSRERAGLDRPPADLSKASALERLMRGFVALGDQTEAALARRLGPDRARE
                     IRGDGWGSRTELSGCPSTGDGK"
BASE COUNT          211 a          595 c          597 g          223 t
ORIGIN      
        1 ggaccgtgga gcaggccgag gcgcgggcca tggcggccat ggatgggtgc cgcgcgccgt
       61 ggtgggtcgc cggcggctgg gccctcgagc tgcacgccgc ggcgcacggc caccggaacc
      121 ggccacatgc cgatctcgag atctcgatcc tcaggcgcga ccagcgtgcg ctgttcgacc
      181 acctcgccgg gtggcagcga tgcgccatgg tccgcccggg cgtgctcgaa cactgggacg
      241 gccggaccct cccggccgag gtgcaccaga tctgggcccg gcgcggagcg ccgctgtcac
      301 cggacccgga ccgcttcgcc gccgacccga cgctcctcga cttcctcctc gaggaggccg
      361 acggcgacac ctggcgcttt cgccgccagc cctcgatcgc gcgcccgctg gcgcagctcg
      421 gcgcgatcac cgcgcgtggc gtcccgttca tccgcccgga ggtcgcgctg ctgtacaagg
      481 ccaagcacct gcgcttcaag gatcagcgcg acttcgacgc cgcggcgcca gccctcgacg
      541 aggccgcgcg tgcgtggctc gcgaccgcgc tcgaccaggc gcacccggac cacccgtggg
      601 gcctcgcgct ggaccgcgcc ggcgcctgac atcaaccgct ggtgcggcga cgaggaacgc
      661 ggcgcgcaag caagtagatg agtgcgccaa tgatgccggc gagcacgatg accagaatcc
      721 acacgacctt gtcgcgctcg ttctcgtatc ggatgcagtc gagcagcatc cagagccaga
      781 ccccggacat cgacaggatc agcaggatca cgatggactc acccaatccc ggcatgccgg
      841 gcagtatcgt ctgagccccc cgctggtgca acgtccgagc cgacggcgcc ctcgaccgcg
      901 cggctacttg ccgtcgccgg tggaagggca cccgctcagc tcggtgcgcg agccccagcc
      961 gtcgccgcgg atctcgcgtg cgcgatcggg gccgagccgc cgggccagcg cggcctcggt
     1021 ctgatcgccg agcgcgacga agccgcgcat cagtcgctcg agcgccgatg ccttgctgag
     1081 atcggcgggc ggccggtcca gcccggcgcg ctcccgggac agccgctgca gcagcgcgtt
     1141 gtactcggcc ggtgagctct tctcgacgat ctcgcggtgc atcgacccgg tcgacagcgc
     1201 gtccgcgccg gcggtgtccc cggtggtctc gacgtagagg ctgtgcacga ggtcctgcca
     1261 gcgccggccc gtctccgcca tcgcggcgtg gaagccatcg acctcttcgg cggtgacgcc
     1321 gctcccgggc gtcggcatgc cgtgctcgaa gtcgggcatg tcgaagcgga ggtggcaggt
     1381 cttgacccac gcagcgagct gctcggggct ggggtcgtgc cacggacggt cgggatcggc
     1441 gccgtcctcg gtggctgccg gcgcggcggt gggttcgagc cgggcgagct gcgcgcggag
     1501 ctcgatcagc tgggcctgct gctcgcgggt gcgggcgagc agctgctcgc gggtggcgct
     1561 gtcgtcggcc gcggtcccgg gcgtggcgct ggcgaccgtg gtggagtcac cgcgcagcgt
     1621 ggcgcg
//
LOCUS       DASVOR010000405         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_179272,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000405 DASVOR010000000
VERSION     DASVOR010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179272"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /locus_tag="VF516_08165"
     CDS             complement(<1..988)
                     /locus_tag="VF516_08165"
                     /inference="COORDINATES: protein motif:HMM:NF019239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1570 domain-containing protein"
                     /protein_id="HEX8107690.1"
                     /translation="MVRVVVCSAITAVAAAGCGAAVPPVPGKGGPAWIELTSEHFTVW
                     TDGDRAEARELVQQMEQLRQVVVGVTFPWVSRGRTLVFALRDDDELAAFSATEQPRAF
                     AMLPGAPLWQPMIALSARTNFQASDRTVAHELTHVISFAAVHHQPRWLAEGMADFFQT
                     LQLDVDRGTADVGGAPTVRGQPRKMAHLIPVEKLFAWKDISAHEEREYSTAWALFTYL
                     INKHRDELVHYLQLLHEVGKPRMKATVEDAAQLWSEAFPSLLLADVDSELRQWLIAGS
                     HVVMHFTVQLQTWSVTERALGDADAYAARGTLCALVANRPAEARTDAAASLAL"
     gene            1136..>1777
                     /locus_tag="VF516_08170"
     CDS             1136..>1777
                     /locus_tag="VF516_08170"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8107691.1"
                     /translation="MTGPADRADEPNEGDGDDDSDDLPPPSAAAHHGFTVDPERAGER
                     IDALIAAVVPALSRAQVQRLIDGAQVRINGAAVAKPGHRVRAGDAVEVDVPPAEPIEL
                     VAEDLPLAVLYEDADLIAIDKPAGLVVHPAAGHARGTLVHALLFHCRDLSGIGGALRP
                     GIVHRLDKDTSGAMVVAKSDRAHAALGAAFAAKSRGEPGGIVREYLGVTAPPPP"
BASE COUNT          244 a          691 c          609 g          233 t
ORIGIN      
        1 ccagcgccag cgaggccgcc gcgtcggtgc gcgcctctgc cggccggttg gcgaccagcg
       61 cgcacagcgt accgcgcgcc gcgtacgcgt cggcatcgcc gagcgcgcgc tcggtgaccg
      121 accaggtctg cagctgcacg gtgaagtgca tcacgacgtg gcttccggcg atcagccact
      181 gccggagctc ggagtcgacg tcggcgagca gcagcgacgg gaaggcttcg ctccacagct
      241 gggcggcatc ctccaccgtg gccttcatgc gcggcttgcc cacctcgtgc agcagctgca
      301 ggtagtgcac gagctcgtcg cggtgcttgt tgatcaggta ggtgaacagc gcccacgccg
      361 tgctgtactc gcgctcctcg tgggccgaga tgtccttcca cgcgaacagc ttctcgaccg
      421 ggatcaggtg cgccatcttc ctcggctggc cgcgaaccgt cggtgcgccc ccgacatccg
      481 cggtcccgcg gtcgacgtcc agctgcaggg tctggaagaa gtccgccatg ccctccgcca
      541 gccagcgcgg ctgatggtgg acggccgcaa acgagatcac gtgggtcagc tcgtgcgcga
      601 cggtgcgatc gctggcctgg aagttggtgc gcgccgacag cgcgatcatc ggctgccaca
      661 gcggagcacc gggcagcatc gcgaacgccc gcggctgctc ggtcgccgag aacgcggcga
      721 gctcgtcgtc gtcgcgcagt gcgaacacca gggtgcggcc gcgcgagacc cacgggaacg
      781 tcaccccgac gacaacctgc cggagctgct ccatctgctg caccagctcg cgggcctccg
      841 cccggtcgcc atcggtccac accgtgaaat gctcgctggt gagctcgatc catgccggcc
      901 cgcccttgcc gggcaccggc ggcacggcgg cgccgcagcc cgccgcggcc actgccgtga
      961 tcgccgagca caccacaacc cggaccatgc aacgcatctt cccctccccg tcgagcatcc
     1021 ccggatcgcc ggtccctccg caagcgcgca cggccggatc gagggcttgt cagggcacgc
     1081 cgcgcgtggt gtcttccctc cggatgcgcc gctgtctcgc cgcccggttt gcgccatgac
     1141 ggggccggcc gaccgcgcgg acgagccgaa cgagggcgat ggcgacgacg acagcgacga
     1201 cctgccgccc ccctcggccg ccgcccacca cggcttcacc gtcgaccccg agcgcgccgg
     1261 cgagcggatc gacgcgctga tcgccgcggt cgtccccgcg ctgtcgcgcg cccaggtcca
     1321 gcggctgatc gacggcgcgc aggtccggat caacggcgcg gcggtcgcca agcccggcca
     1381 ccgggtgcgc gccggcgacg cggtcgaggt cgacgtcccg ccggccgagc cgatcgagct
     1441 ggtcgccgag gacctgccgc tcgccgtgct gtacgaggac gccgacctga tcgccatcga
     1501 caagccggcc ggcctggtcg tgcatcccgc ggccgggcac gcccgcggca cgctggtcca
     1561 tgccctgctg ttccactgcc gggacctcag cggcatcggc ggcgcgctcc gccccggcat
     1621 cgtccaccgg ctcgacaagg acaccagcgg cgccatggtc gtcgccaaga gcgatcgcgc
     1681 gcacgcggcg ctcggcgcgg cgttcgccgc caagagccgc ggcgagcccg gcggcatcgt
     1741 ccgcgaatac cttggtgtca cggccccgcc gccgccg
//
LOCUS       DASVOR010000406         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_471498,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000406 DASVOR010000000
VERSION     DASVOR010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471498"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="VF516_08175"
     CDS             complement(<1..830)
                     /locus_tag="VF516_08175"
                     /inference="COORDINATES: protein motif:HMM:NF018234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8107692.1"
                     /translation="MTRDARVESAIAHWAPRFVSNGVLLQDFQEVTAGLERWDDWCRA
                     WSARAAVHEALGHEALATGHRLTAGEHLTRAGVYYHFAKFMFVHDIAQMREAHGKAVA
                     CRQAASPHVQPPGERVEIPYQGSRLAGILRRPAGAARPPIVIMLPGLDSAKEEFDAYE
                     STFLARGMATLSIDGPGQGEAEYEFPIRGDYEVAVTAVVDWLAGRSDVDPARVGLWGV
                     SIGGYYAPRAAAFEHRLRGCIALAGPYDLGAAWDGLPELTREAFRVRSHLRTQDEARR
                     "
     gene            complement(1187..>1940)
                     /locus_tag="VF516_08180"
     CDS             complement(1187..>1940)
                     /locus_tag="VF516_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8107693.1"
                     /translation="LEPLHDAGISVLPIAGNHDTYLSWQREGYEQAFADLVRWAAPFK
                     VNPGTGRGFARSPYSYSLDFDGVHFALAHVVDQAIDRDVADWLTDDLAAAANARIRLV
                     FGHVPLFSVIRRPARQFVDRFGPILEHGHADFYVAGHEHIAWDETFSLPSGDALRQVT
                     VGCASGFYVFSPSKAAQQRAACERQGARGTLSCTMPNGGRFELARDRGRRWIEHDANT
                     FTVFTVTGDDVQVTPMTVDDDGHLHAFYLRDA"
BASE COUNT          294 a          719 c          666 g          261 t
ORIGIN      
        1 aaccgccgcg cctcgtcctg cgtccggagg tggctgcgca cgcggaacgc ctcgcgggtc
       61 agctcgggca agccgtccca ggccgcgccg aggtcgtagg gcccggcgag cgcgatgcag
      121 ccgcgcagcc ggtgctcgaa cgccgccgcg cgcggcgcgt agtagccccc gatgctgacg
      181 ccccacagcc cgacgcgcgc cggatcgacg tcgctgcggc cggcgagcca gtcgaccacc
      241 gcggtgaccg cgacctcgta gtcgccgcgg atcggaaact cgtactccgc ctcgccctgc
      301 cccggcccgt cgatcgacag cgtcgccatc ccgcgcgcca ggaacgtgga ctcgtaggcg
      361 tcgaactcct ccttggccga atccagaccg gggagcatga tcacgatcgg cggccgggcc
      421 gcgcccgccg gacggcgcag gataccggcg agccggctgc cctgataggg aatctcgacc
      481 cgctcgcccg gcggctgcac gtgcggcgag gccgcctgcc ggcacgccac ggccttgccg
      541 tgcgcctcgc gcatctgcgc aatgtcatgg acgaacatga acttggcaaa gtggtaatac
      601 acgcccgcgc gggtcaggtg ctcgccggcg gtgagccggt ggccggtggc gagcgcctcg
      661 tgcccgagcg cctcgtgcac cgccgcgcgc gccgaccacg cgcggcacca gtcgtcccag
      721 cgctcgagcc ccgccgtcac ctcctggaag tcctgcagca gcacgccgtt cgacacgaac
      781 ctcggcgccc agtgcgcgat cgcagactcc accctggcat cgcgggtcat ggcatcgtcc
      841 tctcaggtca agactagccc agcgcgcagg gcagatcgcg ctcaacaccc acgcgatcgc
      901 gacgcgatgc gaacgcccag cagaacgcgt acacgctcgg ctcgccgtgg gctgctgcgc
      961 ggatgggcgt cggagccgcg cccgccgaat caccacccac ccctgcgcgg gcggcgcgag
     1021 gtatgtgcgg gacaagacct gccaatccaa atcacggtcg cggcgtccga ccccatcctg
     1081 ctcgcggtcg cccacggcag cctcgcgttg gcgtctcccg atcgcgcctc ggaagggttc
     1141 ccatcggacc cgctcgctcc ccggggcccg gctggccccg ggccgatcag gcgtcgcgga
     1201 ggtagaacgc gtgcagatga ccgtcgtcgt cgacggtcat cggggtcacc tgcacgtcgt
     1261 cgccggtcac ggtgaacacg gtgaacgtgt tggcgtcgtg ctcgatccat cgccgacctc
     1321 gatcgcgcgc cagctcgaag cggccaccgt tgggcatcgt gcagctgagt gtcccgcgcg
     1381 ctccctggcg ctcgcatgcg gcgcgctgct gcgctgcctt cgacggcgag aagacataga
     1441 agcccgacgc gcagcccacc gtcacctggc gcagcgcgtc gcccgagggc agcgagaacg
     1501 tctcgtccca cgcgatgtgc tcgtgaccgg cgacgtagaa gtccgcgtgg ccgtgctcga
     1561 ggatcgggcc gaaccgatcg acgaactgcc gcgccgggcg gcggatcacc gagaacagcg
     1621 ggacgtggcc gaacaccagg cgaatacgtg cgttcgcggc ggccgcgaga tcgtcggtca
     1681 gccagtctgc aacgtcccgg tcgatcgcct ggtcgacgac atgcgcgagc gcgaagtgca
     1741 caccgtcgaa gtcgaggctg tagctgtagg gggatcgtgc aaacccccgc ccggtgccgg
     1801 ggttgacctt gaacggcgct gcccagcgca cgaggtcggc gaacgcttgc tcgtagccct
     1861 cgcgctgcca gctcaggtac gtgtcgtgat tgccggcgat cggcaggacc gagatcccgg
     1921 cgtcgtgcag cggctcgagg
//
LOCUS       DASVOR010000407         1807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_237407,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000407 DASVOR010000000
VERSION     DASVOR010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237407"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1094
                     /locus_tag="VF516_08185"
     CDS             27..1094
                     /locus_tag="VF516_08185"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX8107694.1"
                     /translation="MAEPQPGQHRASHVFNVAMLVFGAAMLAWLLRGLDRGDLSAAIA
                     GVGRRFAIILGLDLAAVLLDARALHAFMRPEARMISYGRVVAAQLSGRAVNVVTPLSA
                     LGEATKLAMLVTHAPRVRVVSAIVLFNLAALYLSVIVMVIGVPITLLLVDLPRALQVT
                     AGIGLAAAIALVIAVGVLIERGATATLTGLLHRVRVISRERRDDWQARLQELDRHLAE
                     LHDQRGAGTWRGVLWLIASRLVTWTSTLTLIGAIGVEIAPRLVAGVLSVGVLIQWIAA
                     AVPFGLGVADTGNYALYDLLGASGAHGAVVTMLNRARSLSVAVVGFAVMTVVHTLNRL
                     AVARMHARLRDRRAQLQAPRG"
     gene            complement(1091..1435)
                     /locus_tag="VF516_08190"
     CDS             complement(1091..1435)
                     /locus_tag="VF516_08190"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HEX8107695.1"
                     /translation="MTPDLPSLPHNDQGSPVFREPWEAQAFAMAVQLHQAGHFTWAEW
                     AAALAEQIRQAQAAGDPDLGDTYYRHWLAALERLVAAKGLISAEELARRKAEWDAAAR
                     ATPHGQPIELAR"
     gene            complement(1432..>1807)
                     /locus_tag="VF516_08195"
     CDS             complement(1432..>1807)
                     /locus_tag="VF516_08195"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:NF014289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3-like domain-containing protein"
                     /protein_id="HEX8107696.1"
                     /translation="GGPAPLQDGALRSDGVAGMLGKRRAARLDDPVPPRFHVGQTVVA
                     RNLHSVGHTRLPRYVRGRRGEVVIDHGVFIFPDTHAAGQGTKPQHVYSVRFTARELWG
                     PDASARDTVHVDLWDDYLEAAP"
BASE COUNT          202 a          651 c          679 g          275 t
ORIGIN      
        1 ccagcaactg caggtaacct ggagccatgg cggaacccca gcccgggcag caccgcgcga
       61 gccacgtgtt caacgtggcg atgctggtgt tcggggccgc catgctggcg tggctgctgc
      121 gcgggctcga tcggggcgat ctgtccgcgg cgatcgccgg ggtcggccgc cggttcgcga
      181 tcatcctggg gctcgacctg gcggcggtcc tgctcgatgc gcgcgccctg cacgcgttca
      241 tgcgcccgga agcccggatg atctcgtatg gccgggtggt ggcggcccag ctcagcgggc
      301 gcgcggtcaa cgtggtcacc ccgctcagcg cgctgggcga ggccaccaag ctcgccatgc
      361 tggtgaccca cgcgccgcgc gtccgggtcg tgtccgcgat cgtgctgttc aacctggccg
      421 cgctctacct gtcggtcatc gtcatggtga tcggcgtgcc gatcacgctg ctgctggtcg
      481 atctgccgcg ggcgctgcag gtcaccgcgg ggatcggcct cgcggcggcg atcgcgctgg
      541 tcatcgcggt cggcgtgctg atcgagcgcg gcgcgacggc cacgctgacc ggcctgctcc
      601 accgcgtgcg cgtgatctcg cgggagcgcc gcgacgactg gcaggcccgc ctccaggagc
      661 tcgaccgtca cctggccgag ctccacgacc agcgtggcgc cgggacctgg cggggcgtgc
      721 tgtggctgat cgcctcgcgg ctggtcacct ggacgtcgac cctgacgctg atcggagcga
      781 tcggtgtgga gattgcgccg cgcctggtgg cgggagtgct gtcggtcggc gtgctgatcc
      841 agtggatcgc ggcggccgtg ccgttcggcc tcggcgtcgc cgataccggc aactacgcgc
      901 tgtacgacct gctcggcgcg tccggcgccc atggcgcggt ggtgaccatg ctgaaccggg
      961 cgcgcagcct gtcggtcgcc gtggtcggct tcgccgtcat gacggtggtc cacacgctga
     1021 accggctggc ggtggcgcgg atgcatgccc ggctgcgcga ccgccgcgcc cagctgcagg
     1081 ccccgcgggg ttagcgcgcc agctcgatcg gttggccgtg cggcgtcgcg cgcgccgcgg
     1141 cgtcccactc ggccttgcgg cgggccagct cctcggcgct gatcaggccc ttggcggcga
     1201 ccaggcgctc gagcgccgcc agccagtgcc ggtagtaggt gtcgccgaga tccggatcgc
     1261 cggcggcctg ggcctgccgg atctgttcgg cgagcgccgc cgcccactcg gcccaggtga
     1321 agtggccggc ctggtgcagc tgtaccgcca tcgcgaacgc ctgcgcctcc catggctcgc
     1381 ggaacaccgg gctgccctgg tcgttgtgcg gcaggctggg cagatccgga gtcacggcgc
     1441 cgcctccagg tagtcatccc atagatcgac gtgcacggtg tcgcgcgccg aggcgtcggg
     1501 gccccacagc tcgcgcgcgg tgaaccgcac gctgtagacg tgctggggct tggtgccctg
     1561 ccccgccgcg tgggtatccg ggaagatgaa caccccgtgg tcgatcacca cctcgccgcg
     1621 ccggccgcgc acgtagcgcg gcagccgggt gtggccgacc gagtggaggt tgcgggccac
     1681 gaccgtctgg ccgacgtgga accggggcgg caccggatcg tcgagccgcg ccgcacgccg
     1741 cttgccgagc atgcccgcca cgccgtcgct gcgcagcgcg ccgtcctgca gcggcgccgg
     1801 gccgccc
//
LOCUS       DASVOR010000408         2171 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_120643,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000408 DASVOR010000000
VERSION     DASVOR010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2171
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120643"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VF516_08200"
     CDS             <1..243
                     /locus_tag="VF516_08200"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8107697.1"
                     /translation="AGPDTESVGGSRREIERMLAALATCSPTARKVFRLVYATPGGTA
                     SHAAKEVGLSLQRVRQILCETRRHIRRTLALGEETP"
     gene            240..782
                     /locus_tag="VF516_08205"
     CDS             240..782
                     /locus_tag="VF516_08205"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8107698.1"
                     /translation="MNRDDLELYVMGLYDGDLAALERELADDPAARAALCASFQEGDG
                     IHARYYLAQELIAGEALDDRLESHWFTEAEILAIARQVLDVLVYLQGLSPMVIHRDIK
                     PANLLVRPDGAIAVVDFGAAHVHGATAASTAIGTFGYMPIEQLAGEVDATTDLIAEDG
                     ARASRVRHGIVEDPLPPGVT"
     gene            824..1243
                     /locus_tag="VF516_08210"
     CDS             824..1243
                     /locus_tag="VF516_08210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107699.1"
                     /translation="MLARIGGVAATLPDAIDGTVTVHVTHMAWDRALVVVLETAGLGH
                     RYQAAGKQLRIAPLAELDAERERTPHGFLEVSRRANTRVAIDGVDVEVAAGIPLQLTP
                     GSHRVSFTRPDGHVSTAVVEIAAGRTAQLHDGSARTD"
     gene            complement(1249..1998)
                     /locus_tag="VF516_08215"
     CDS             complement(1249..1998)
                     /locus_tag="VF516_08215"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEX8107700.1"
                     /translation="MATAGIVIIGDEILTGKFADENAAFLIGELRALGVDLRRIAVIP
                     DVLDDIAATVTEMSARFDHVFTSGGVGPTHDDVTMAGIAQGFGTRVVREPVLEERVRA
                     YWGAKLAPPHLRLAEVPAGAELVYGKDQVWPVVAYRNVYILPGVPALFRRKFIDIRDR
                     FRAQPVTVARAYLDADEGQIAGDLDAVVAAFPAVRIGSYPRFTERDFKVLITFEGPAA
                     SDVAGALAMLVERIGPRVVRQEPPALVTGAD"
BASE COUNT          315 a          799 c          800 g          257 t
ORIGIN      
        1 gccggcccgg acaccgagag cgtgggcggc agccgccggg agatcgagcg catgctcgcc
       61 gcgctggcga cgtgctcgcc gaccgcgcgc aaggtgttcc ggctggtcta cgccacgccg
      121 ggcggcaccg cgagccacgc cgcgaaggag gtcgggctgt cgctgcagcg ggtccgccag
      181 atcctgtgcg agacccgccg ccacatccgg cgcacgctcg cgctggggga ggagacgcca
      241 tgaaccgcga cgatctcgag ctctacgtga tgggcctcta cgacggcgac ctcgccgcgc
      301 tcgagcgcga gctcgccgac gaccccgccg cgcgcgcggc cctctgcgcg agcttccagg
      361 aaggcgacgg gatccacgcc cggtactacc tcgcccagga gctgatcgcc ggcgaggcgc
      421 tcgacgaccg gctcgagtcg cactggttca ccgaggccga gatcctcgcg atcgcgcgcc
      481 aggtgctcga cgtgctggtc tacctgcagg ggctgtcgcc gatggtgatc caccgcgaca
      541 tcaagcccgc caacctgctg gtccgccccg acggcgcgat cgccgtcgtc gacttcggcg
      601 ccgcccacgt ccacggcgcg accgccgcgt cgacggcgat cggcacgttc ggctacatgc
      661 cgatcgagca gctcgccggt gaggtcgacg cgaccaccga cctcatcgcc gaggatggcg
      721 cgcgggccag ccgcgtgcgg cacggcatcg tcgaggatcc gctgccgccg ggcgtgacgt
      781 agacatcgac ttccagaagg cgccggtccg cgaggtgatc cagatgctcg cgcgcatcgg
      841 cggcgtcgcg gccacgctgc cggacgcgat cgacggcacc gtgaccgtgc acgtcaccca
      901 catggcgtgg gatcgcgcgc tggtcgtcgt gctcgagacc gcgggcctgg gccaccgcta
      961 ccaggcagcg ggcaagcagc tgcggatcgc gcccctcgcc gagctcgacg ccgagcgcga
     1021 gcgcacgccg cacgggttcc tggaggtgag ccgccgtgcg aacacccggg tcgcgatcga
     1081 cggcgtcgac gtcgaggtcg cggccggcat cccgctccag ctcacgccgg gcagccaccg
     1141 cgtgtcattc acccggcctg atggtcacgt gtcgacggcc gtggtcgaga tcgcggcggg
     1201 ccgcaccgcg cagctgcacg acggatcggc gcgaacggac tgatcggcct aatccgcccc
     1261 ggtcaccagg gccgggggct cctggcgcac gacccgcggg ccgatccgct cgaccagcat
     1321 ggccagcgcg ccggcgacgt cgctcgccgc ggggccctcg aacgtgatca gcaccttgaa
     1381 gtcgcgctcg gtgaaccgcg ggtacgagcc gatccgcacc gcggggaacg ccgcgaccac
     1441 cgcgtcgaga tcgcccgcga tctggccctc gtcggcgtcg aggtaggccc gggcgacggt
     1501 caccggctga gcgcggaacc gatcgcggat gtcgatgaac ttgcggcgga acagcgccgg
     1561 cacgccgggc aggatgtaga cgttgcggta ggcgacgacc ggccagacct ggtccttgcc
     1621 gtagaccagc tcggccccgg cggggacctc ggcgagccgc aggtgcggcg gggcgagctt
     1681 ggcgccccag taggcgcgga cgcgctcctc gaggaccggc tcgcggacga cgcgggtgcc
     1741 gaagccctgg gcgatcccgg ccatggtgac gtcgtcgtgg gtcgggccga cgccgcccga
     1801 ggtgaacacg tgatcgaagc gcgccgacat ctcggtgacg gtggcggcga tgtcgtcgag
     1861 cacgtcgggg atcacggcga tccgccgcag gtcgacgccg agcgcgcgca gctcgccgat
     1921 caggaacgcg gcgttctcgt cggcgaactt gccggtcaag atctcgtcgc cgatgatcac
     1981 gatgccggcg gtcgccacgc ggcgaccata tcaccggcgc agcgtgagca cgaccacgac
     2041 ggcggccgcc agcaagaggc cgacgccgcc caggccgacc cacaccagcg agccggcgcc
     2101 cgggtcggcc ggttcgagga gtcgctcggc cgcggcggac gcgggcgacg cggccgcgcc
     2161 ggcggcgggc g
//
LOCUS       DASVOR010000409         1599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_529859,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000409 DASVOR010000000
VERSION     DASVOR010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529859"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1599)
                     /locus_tag="VF516_08220"
     CDS             complement(<1..>1599)
                     /locus_tag="VF516_08220"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8107701.1"
                     /translation="TAAQLATDHADDAGQSMRFHRLVLDVDPHDPGASATLEQFYEDN
                     QDKSGLIEVLQHKLAHTDNEPASVELLRRIARVSEDGAQDVENATLHYQKILELQPEN
                     KDALEALGRIYESTEQWAELIDVTRRLIKVTSDRNTKALLYFRCGSVMEAKFGREQDA
                     IRYYDAAIKTSNTCLPAVHGLRDLYRRHEDWPRVIETLELEVKLWQDDKERAGVYAQI
                     GGIYDKQLGDTPRAMEYYDSALAVDPDCLPANQALFEHFFHQGAWDRAQPIANALAQK
                     AMRDGDPTTRSEFYRKRGVVARMTGDPRAAAESFIVALEIRPGNSDALDDLGALARER
                     PDVWDFDATYRELDKVYKKRDDAGALLARVHVGRAAIRERDGDLDQAAELYRAAAGLV
                     PGDFTVLSALVDFHTDMRHWSEAIAAIQTFVTGDAATPADRIAALMRQAEIHADGELD
                     TGRAIAVLHEVLERDPAHQDAYYALAQQYYLAGRTTDARVAIDRVIELATAPGQPLLP
                     AALARYYYYKGRILDAAGDSRAAAP"
BASE COUNT          186 a          535 c          609 g          269 t
ORIGIN      
        1 cggcgcggcg gcgcgcgagt cgccggcggc gtcgaggatc cggcccttgt agtagtagta
       61 gcgggcgagc gcggcgggca gcagcggctg gccgggcgcg gtcgcgagct cgatcacgcg
      121 gtcgatcgcg acccgggcgt cggtggtgcg gccggcgagg tagtactgct gggcgagcgc
      181 gtagtaggcg tcctggtgcg ccggatcgcg ctcgagcacc tcgtgcagca ccgcgatcgc
      241 gcggccggtg tcgagctcgc cgtcggcgtg gatctcggcc tgccgcatca gcgcggcgat
      301 ccggtcggcg ggcgtcgcgg cgtcgccggt gacgaacgtc tggatcgcgg cgatcgcctc
      361 gctccagtgc cgcatgtcgg tgtggaagtc gaccagcgcc gacagcacgg tgaagtcgcc
      421 gggtacgagc cccgccgccg cgcggtacag ctcggcggcc tggtcgagat cgccatcgcg
      481 ctcgcggatc gcggcgcggc cgacgtggac gcgggcgagc agcgcgccgg cgtcgtcgcg
      541 cttcttgtag accttgtcga gctcgcggta ggtcgcgtcg aagtcccaca cgtcgggccg
      601 ctcgcgggcg agcgcgccga ggtcgtcgag cgcgtcgctg ttgcccggcc ggatctcgag
      661 cgcgacgatg aagctctcgg cggccgcgcg cggatcgccg gtcatccggg cgaccacgcc
      721 gcgctttcgg tagaactcgc tgcgcgtcgt cgggtcgccg tcgcgcatcg ccttctgggc
      781 cagcgcgttg gcgatcggct gcgcccggtc ccacgcgccc tggtggaaga agtgctcgaa
      841 cagcgcctgg ttggcgggca ggcagtcggg gtcgaccgcg agcgcgctgt cgtagtactc
      901 catcgcgcgc ggggtgtcgc cgagctgctt gtcgtagatc ccgccgatct gggcgtagac
      961 cccggcgcgc tccttgtcgt cctgccatag cttgacctcg agctccaggg tctcgatcac
     1021 gcgcggccag tcctcgtgcc ggcggtacag gtcgcgcagg ccgtggaccg cgggcaggca
     1081 ggtgttcgag gtcttgatcg ccgcgtcgta gtagcggatc gcgtcctgct cgcggccgaa
     1141 cttggcctcc atcaccgagc cgcaccggaa gtacaacagc gccttggtgt tgcggtcgct
     1201 ggtgaccttg atcaggcggc gggtgacgtc gatcagctcg gcccactgct cggtcgactc
     1261 gtagatccgg ccgagcgcct cgagcgcgtc cttgttctcg ggctggagct cgaggatctt
     1321 ctggtagtgc agggtcgcgt tctcgacgtc ctgcgcgccg tcctcggaca cccgcgcgat
     1381 ccgccggagc agctcgaccg acgccggctc gttgtcggtg tgcgccagct tgtgctggag
     1441 cacctcgatc agcccggact tgtcctggtt gtcctcgtag aactgctcga gcgtggccga
     1501 cgcgccggga tcgtggggat cgacgtcgag caccaggcgg tggaaccgca tcgactggcc
     1561 ggcgtcgtcg gcgtggtcgg tggcgagctg ggccgcggt
//
LOCUS       DASVOR010000410         2447 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_354572,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000410 DASVOR010000000
VERSION     DASVOR010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354572"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2447
                     /locus_tag="VF516_08225"
     CDS             <1..>2447
                     /locus_tag="VF516_08225"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107702.1"
                     /translation="DALERQAELDVAAGGVDRHVEIARLLSGPLGRALEALDRWSTVL
                     AAEPEHPIAIAAVEAALGDPDLRVAAAAILRPVYAASAHYDRIAELQLRAADWADDPA
                     SKLRAYREVVQLREHRLDDVPGAFEAQLLALHCAATEPELASVIAETERLAGELGREA
                     DLIDAYRAVAPDVLDAEIQRRLYLDVADLSRAVRNDLPLAGEYYQKVLDSQPDDRRAL
                     VALEAIYRDLNDDERLTEILLRQADSPNADVDERVGALVEAASLYVSLARPDDAIATW
                     EQVLAVAPERRDAVEALEALYREQGRWPDVVELYERRLGFATSIEEAVALRVQLGEIH
                     ERQLHDVETAIDNYASALGGDPHHAAALAAVERYLVDPDARAVAAEVLEPIYVAQQRW
                     TDLIRVYEAKLESASDPRERLRLTRFVARLFEEQLEDFEKASRWYARLFRELPSDPGI
                     RDQLQRLASIVDNWAFVAETYQDYLDAESGDSSDLRDVAIAAATIYDRRLGEVDRAFH
                     AYRRALAIADDGVPDARELVRRLEDLLGRAQRWPELIAIYDDVIAAGEDDLRREALIK
                     RARLYEDGLGDAGRAIDGWREVVIATEDGGTAVTEHAYREAVTELERLYRARGEWREL
                     VALFEARLARATHPADSAELRLRLAEVYETQLADVPAALDQYEHVIAEGARWEAAVAA
                     LERLVVQDEHRERIAELLEPVYRREDWWQKLVVILDAKLDYVRDPADQVRTLHEIARI
                     HEDRGGALDLALQALARAWRIDVADDDSLGKLMVLAGTLAAWDEVVATLEDGAAAAPS
                     SALAAALWARAAEVHEQR"
BASE COUNT          327 a          923 c          910 g          287 t
ORIGIN      
        1 gacgcgctgg agcgccaggc cgagctcgac gtcgccgccg gcggggtcga tcgccacgtc
       61 gagatcgcgc gcctgctgtc cgggccgctc ggccgggcgc tcgaggcgct cgaccgctgg
      121 tcgaccgtgc tcgccgccga gcccgagcac ccgatcgcga tcgcagcggt cgaggccgcg
      181 ctcggcgatc ccgacctgcg ggtcgccgcc gccgcgatcc tgcgcccggt ctacgccgcc
      241 agcgcgcact acgaccggat cgccgagctc cagctgcgcg ccgcggactg ggccgacgat
      301 ccggcgtcca agctgcgggc gtaccgcgag gtcgtccagc tccgcgagca ccggctcgac
      361 gacgtgccgg gcgcgttcga ggcccagctg ctcgcgctgc actgcgcggc gaccgagccc
      421 gagctcgcct ccgtgatcgc cgagaccgag cggctcgccg gcgagctcgg ccgcgaggcc
      481 gatctgatcg acgcctaccg cgcggtcgcg cccgacgtgc tcgacgccga gatccagcgc
      541 cggctgtacc tcgacgtcgc cgacctgtcg cgcgcggtgc gcaacgacct gccgctggcc
      601 ggcgagtact accagaaggt gctcgacagc cagcccgacg accgccgcgc gctggtcgcg
      661 ctcgaggcga tctaccgcga cctcaacgac gacgagcggc tgaccgagat cctgctgcgc
      721 caggccgaca gccccaacgc cgacgtcgac gagcgggtcg gcgcgctggt cgaggccgcg
      781 agcctgtacg tctcgctggc ccggcccgac gacgcgatcg cgacctggga gcaggtgctg
      841 gcggtcgcgc ccgagcgccg cgacgcggtc gaggcgctgg aggcgctgta ccgcgagcag
      901 ggccggtggc ccgacgtggt cgagctctac gagcgccgcc tcgggttcgc gacctcgatc
      961 gaggaggcgg tcgcgctgcg ggtccagctc ggcgagatcc acgagcgcca gctccacgac
     1021 gtcgagaccg cgatcgacaa ctacgcctcg gcgctcggcg gcgatcccca ccacgccgcc
     1081 gcgctcgccg cggtcgagcg ctacctggtg gaccccgacg cgcgggcggt cgccgccgag
     1141 gtgctcgagc cgatctacgt cgcccagcag cgctggaccg atctgatccg ggtctacgag
     1201 gccaagctcg agagcgcgag cgatccgcgc gagcggctgc ggctgacccg gttcgtcgcc
     1261 cggctgttcg aggagcagct cgaggacttc gagaaggcga gccggtggta cgccaggctg
     1321 ttccgcgagc tgcccagcga tccgggcatc cgcgaccagc tgcagcgcct ggcgtcgatc
     1381 gtcgacaact gggcgttcgt cgccgagacc taccaggact acctcgacgc cgagtccggc
     1441 gactcgtccg acctgcgcga cgtcgcgatc gccgcggcga cgatctacga ccgccggctc
     1501 ggcgaggtcg accgggcgtt ccacgcctac cgccgcgcgc tggcgatcgc cgacgacggc
     1561 gtgcccgacg cgcgcgagct ggtccgccgg ctcgaggacc tgctcggccg cgcccagcgc
     1621 tggcccgagc tgatcgcgat ctacgacgac gtgatcgccg ccggcgagga cgacctgcgg
     1681 cgcgaggcgc tgatcaagcg ggcgcggctg tacgaggacg gcctgggcga cgccggccgc
     1741 gcgatcgacg gctggcgcga ggtcgtgatc gcgaccgagg acggcggcac cgcggtcacc
     1801 gagcacgcct accgcgaggc ggtcaccgag ctcgagcggc tgtaccgcgc gcgcggcgag
     1861 tggcgcgagc tggtcgcgct gttcgaggcc cggctggcgc gcgcgaccca ccccgccgac
     1921 agcgccgagc tccggctccg gctcgccgag gtctacgaga cccagctcgc cgacgtcccg
     1981 gccgcgctcg atcagtacga gcacgtgatc gccgagggcg cgcgctggga ggccgcggtc
     2041 gccgcgctcg agcggctggt ggtccaggac gagcaccgcg agcggatcgc cgagctgctc
     2101 gagccggtct accgccgcga ggactggtgg cagaagctgg tcgtgatcct cgacgccaag
     2161 ctcgactacg tgcgcgaccc ggcggaccag gtccgcacgc tgcacgagat cgcccggatc
     2221 cacgaggacc gcggcggcgc gctcgacctc gcgctccagg cgctcgcccg ggcgtggcgg
     2281 atcgacgtcg ccgacgacga cagcctcggc aagctgatgg tgctcgccgg cacgctggcg
     2341 gcgtgggacg aggtggtcgc caccctcgag gacggtgcgg cggcggcgcc gagctcggcc
     2401 ctcgcggcgg cgctgtgggc cagggcggcc gaggtccacg agcagcg
//
LOCUS       DASVOR010000411         5635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412363,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000411 DASVOR010000000
VERSION     DASVOR010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5635
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412363"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="VF516_08230"
     CDS             <1..812
                     /locus_tag="VF516_08230"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107703.1"
                     /translation="DGAWYLRAYDDDGRAVGSAHSPPPHIDSISQSWAILSGAGDARR
                     DDQALAAAERMLVRDSDRLVLLLAPPFGPHGPDPGYIAAYPPGVRENGGQYTHAAAWL
                     GWAHAARGDGETAHRIAGLLNPLERTRTPAEVARYRVEPYVLAGDVYAGPWAGRGGWT
                     WYTGAAAWTWRLILEEILGVRRRRGALEVEPCIPRAWDGFDAWIRAGQLTVHVIVRNP
                     EHVARGIAAVSLDGQPVERARVELAGTGERILEIQLGTSERGRGNCTGSVV"
     gene            809..1177
                     /locus_tag="VF516_08235"
     CDS             809..1177
                     /locus_tag="VF516_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107704.1"
                     /translation="MTRSRALVADDDLEILDMVSRALSQLGLEVITASSGSDLLDKVA
                     EQGPFDLVVTDVAMPWMSGLQVMHSARTAGMRCPVIVITALRDSRTGDQVETLGAQVR
                     RLNKPFSMGELEEAVRASLG"
     gene            1217..3064
                     /locus_tag="VF516_08240"
     CDS             1217..3064
                     /locus_tag="VF516_08240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8107705.1"
                     /translation="MSSRDRVVPGAQPRIPSIVLLCLPGMSRQTMIEPGEHQRTPREH
                     PVTADARTRSGMLAMVANPGLSGRAVRRVLLIALAVPVICLFAAWAQNAGLYRPPGAS
                     VLAAVGGCLVAVLSALWLGRWLYRTVVERRWSDDRLRELRDYGEFFDRASFGAWIGSA
                     DGVLKRVNEAFARMHGCTRAEAEGRPLAEFFPEHRREELATNVRIVHSRGHCRWESEN
                     LRKDGTTFPVLVDSGAVYEPDGEVRFRATYIQDITEDKRAEVELARLAAIVRSTDDAI
                     MSTTLDGIVVDWNHAAERIFGYTAEEMIGRSIDGIVPEDHRAEQDALIAKTMAGEPVV
                     GFETIRVCKDGQHIPVALTKSVVRDSSGRVNGISVIERDISVLKQFEREREEWSAIVA
                     HDLRQPASVIRLAAGMLRGLGGEPQKQILARLESACRRLDQMIDDLLDVSQIEARKLV
                     MRPTRLRVATLIDDVLAVAPELARRCTVRLEDSAVETWADAGRFSQVLSDLLSNADKY
                     SYPGTAIDVHVKRTEAMVEVTVTNEGPGIEPDEIPQLFSRFTRTRAARHSPVPGLGLG
                     LYISCGLIEAQGGRMWAESTPGQRTSFHFTLPGAPPAEPRPETGNEAGN"
     gene            3464..3976
                     /locus_tag="VF516_08245"
     CDS             3464..3976
                     /locus_tag="VF516_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107706.1"
                     /translation="MTGKLRAQDVPIGTGKLAEVYFGVTEEMLAPRAVRIQDAVTNTH
                     FGGTISMFVHTGCWDSGLMYVAPGGDTAVFSMEQTDDGTADEWYGPTQEFYFIVAGEF
                     TVSYDTDAERLRRKQSPKYVVHAGEYAVHPVGWKYQVQCTSPTPGTFLWCKHIPPGLA
                     IQERLQMPLK"
     gene            complement(3969..4757)
                     /locus_tag="VF516_08250"
     CDS             complement(3969..4757)
                     /locus_tag="VF516_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013405.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107707.1"
                     /translation="MADSEPETLETGDILRDWNARDYLDYYYGNPSVPDDEAMMFRFI
                     ARGLADIGRHFEAGLELGCGPVLCRAAQAIPWVERLDMADFQDSTLEEIRRWIRRDPG
                     AFDWSIFLDGARGVLDAAGRRLGSLAEREAAMRARIHPVSCDLRDDLPLGKPVQYPLV
                     TSYYCAEWVVPTFAGWQTTMRRFASLVSSGGWLFLAGVHATEYCVLNGRRVPCARVTH
                     DDVRRVLGDVGFDPATVRLDVTPGLRPQVSGIHGTFVAYAQRVI"
     gene            4870..5502
                     /locus_tag="VF516_08255"
     CDS             4870..5502
                     /locus_tag="VF516_08255"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8107708.1"
                     /translation="MIALYSSPGTAGMVVHWLLLELEVPYTLQRLDLAAGEHKQPAYL
                     AVNPAGVVPAVVIDGQPVCEAAAIALHLADAYPARGFAPAIGTPARAAYYRWMFFLAN
                     TMQPAFRAWFYPTEPAGEAHVESVKVQARSRIEGGWALLDAHLAAHGPYLLGDQITTP
                     DFMLTMLMRWSRNMARTATNWPTLARLATAMKARPSFKELYAREGLTDWT"
BASE COUNT          887 a         1941 c         1962 g          845 t
ORIGIN      
        1 gggacggcgc ctggtacctg cgcgcgtacg acgacgacgg acgcgcggtc ggctcggcgc
       61 actctccgcc gccgcacatc gactcgatct cgcagtcctg ggcgatcctg tccggcgccg
      121 gtgacgctcg gcgcgatgac caggcgctcg ccgccgccga gcgaatgctg gtccgcgatt
      181 cggaccgcct ggtcctcctg ctcgccccac cgttcggacc ccacggcccc gatccgggct
      241 acatcgcggc ctatccgccc ggcgtccgcg agaacggtgg ccagtacacg cacgcggcgg
      301 cctggctggg ctgggcgcac gccgcgcgcg gtgacggcga gaccgcgcac cggatcgccg
      361 ggttgctcaa cccgctggag cgcacccgga cgccggccga ggtcgcgcgc taccgcgtcg
      421 agccctacgt gctcgccggt gacgtctacg ccggaccgtg ggccggccgc ggcgggtgga
      481 cctggtacac cggcgccgcc gcctggacct ggcgattgat cctcgaggag atcctcggtg
      541 tgcgccggcg acgcggcgcg ctcgaggtcg agccctgcat cccacgtgca tgggatggtt
      601 tcgatgcctg gatccgagca ggacagctca ccgtgcacgt gatcgtgcga aatcccgagc
      661 acgtggcgcg cggcatcgcc gcggtctcgc tcgacgggca gccggtcgag cgcgcgcggg
      721 tcgagctcgc cggcaccggg gagcggatcc tggagatcca gctcggaacc tccgagcgcg
      781 ggcgcggcaa ttgcaccggt tcggtcgtgt gacccgaagc cgcgcgctgg ttgccgacga
      841 tgatctcgag atcctcgaca tggtttctcg cgcgctaagc cagctcggtc tcgaggtgat
      901 caccgcgtcc tccggatccg atctgctgga caaggtcgca gagcaaggtc cgttcgatct
      961 cgtcgtgaca gacgtcgcga tgccgtggat gtccggtctc caggtcatgc actccgcacg
     1021 caccgccgga atgcggtgcc cggtcatcgt gatcacggcg ctccgtgact ccaggacggg
     1081 cgatcaggtg gagacgctgg gcgcacaggt gcggcggctg aacaagccgt tttcgatggg
     1141 cgagctggaa gaggcagttc gagcgtcgct cgggtgattc gactagagat caccgtggcg
     1201 ctcggcggaa gtctggatgt cctcccgcga ccgcgtagtc ccgggcgccc agccgagaat
     1261 acctagtatt gtcttgcttt gccttccagg catgtcgagg cagaccatga tcgagccggg
     1321 agagcatcag cgaacgccgc gggagcatcc ggtcacggcc gacgcacgca ccaggtccgg
     1381 gatgctggcg atggtggcga acccgggcct ctcaggccgc gctgtccgcc gtgtgctcct
     1441 gatcgcgctc gcggtcccgg tgatctgctt gttcgctgcg tgggcgcaga atgccggact
     1501 gtaccgccca ccgggcgcct ccgtcctcgc ggcggtgggg ggatgtctcg tcgccgtcct
     1561 ctccgcgctc tggctcggcc gctggcttta ccgcaccgtc gtggagcggc gctggagcga
     1621 cgatcggttg cgcgagctgc gcgattacgg ggaattcttc gaccgggcaa gctttggcgc
     1681 atggatcggg tccgcggacg gtgtcctgaa acgggtgaac gaagcgttcg cccggatgca
     1741 tggctgcacg cgggccgagg ccgaggggag gccgctggca gagttcttcc ccgagcaccg
     1801 gcgcgaggaa ctcgcaacca acgtccgcat cgtccacagc cgcggccact gccgatggga
     1861 atcggagaac ctccgcaagg acggcaccac cttcccggtc ctcgtcgatt caggcgcggt
     1921 ctacgagccg gacggtgagg tccggttccg cgccacctac atccaggaca tcacggagga
     1981 caagcgcgcc gaggtcgagc tcgcccggct ggcggcgatc gtgcgctcga cggacgacgc
     2041 catcatgtcg acgacgctcg acgggatcgt ggtcgactgg aaccacgctg ccgagcgcat
     2101 cttcggctac acggccgagg agatgatcgg ccggtcgatc gacggcatcg tccccgagga
     2161 tcaccgggcc gagcaggacg ccctgatcgc gaagacgatg gccggtgagc cggtggtcgg
     2221 gttcgagacc atccgcgtgt gcaaggacgg gcagcacatc ccggtcgcgc tgaccaagtc
     2281 cgtggtccgc gactcctcgg gccgtgtcaa cggcatctcg gtgatcgaac gcgacatcag
     2341 cgtgctcaag cagttcgagc gcgagcgcga ggaatggtcg gcgatcgtcg cgcatgatct
     2401 ccgccagccc gcgtcggtga tccggctcgc ggccgggatg ctccggggcc tcggcggcga
     2461 gccgcagaag cagatactcg cgcgcctcga gagcgcctgc aggcgcctcg accagatgat
     2521 cgacgatctg ctcgacgtgt cgcagatcga agcgcgcaag ctggtcatgc ggccgacccg
     2581 actccgcgtc gcgacactga tcgacgacgt cctcgctgtc gcgccggagc tcgcgcgccg
     2641 ctgcaccgtg cggctcgagg acagcgcggt cgagacgtgg gccgacgctg gacgattcag
     2701 ccaggtgctg tcggacctgc tgtcgaacgc cgacaagtac agctaccccg gaacggcgat
     2761 cgacgtccac gtcaagcgca cggaggccat ggtggaggtc accgtgacga acgagggccc
     2821 tggcatcgag cccgatgaaa tcccgcagct gttctcgcgg ttcacgcgaa cccgcgcggc
     2881 gcggcacagt ccggtcccag gactcgggct cgggctctac atctcctgcg ggctgatcga
     2941 ggcacagggc ggacggatgt gggcggagag cactcccggg caacggacct cgttccactt
     3001 cacccttccc ggggcgccgc cggccgagcc acggccagaa acggggaatg aagctggtaa
     3061 ctgaaggtcc acttcgcagt caccgtcgtc tcggccgaac gcgtccggat caccgtctcc
     3121 gataccggca tcgggattcc catcgacatg ggtgggggtc gtgggcttcc acagcattcc
     3181 cggtgaaggt tcgcagttct ggatcgagat gcctgctcga catgcgcccg agacaccggc
     3241 gctacccccg atctcgcgcc atcgcaccga ggtgatgagc gcgacggcga gatcgatcac
     3301 gcgcccggct ggcgggcaca cctgcgcgac ttcaccgtga tcgatgcacc cggcgaccac
     3361 gattccatgc tcgcaccgcc ccggggtcga cgagctcgcc cggctgctgg acgatgtcat
     3421 ccacggctcg tggcggtggc ggcagcgtga tacgaccggc cgcatgacgg gaaagctcag
     3481 agcgcaagac gtgccgatcg gaaccggcaa gctcgccgag gtatatttcg gggtgaccga
     3541 ggagatgctc gcgccgcgcg cggtgcggat ccaggacgcg gtcaccaaca cccacttcgg
     3601 cggcacgatc tcgatgttcg tgcacaccgg ctgctgggac tcgggcctga tgtacgtcgc
     3661 tcccggtggc gacaccgcgg tgttctcgat ggagcagacc gacgacggca cggccgacga
     3721 gtggtacggc ccgacgcagg agttctactt catcgtcgcc ggcgagttca cggtgagcta
     3781 cgacaccgac gccgagcggc tccggcgcaa gcagagcccg aagtacgtcg tccacgccgg
     3841 cgagtacgcg gtgcacccgg tcggctggaa gtatcaggtc cagtgcacca gcccgacccc
     3901 gggcacgttc ctgtggtgca agcacatccc gccggggctc gcgatccagg agcgtctgca
     3961 gatgccactc aaatgacgcg ctgcgcgtac gccacgaagg ttccgtggat accggacacc
     4021 tgcggtcgca gccccggcgt cacgtcgagc cggacggtgg cggggtcgaa cccgacgtcg
     4081 ccgagcacgc ggcggacgtc gtcgtgggtg acccgcgcgc acgggacgcg ccggccattg
     4141 agcacgcagt actcggtggc gtgaacgccg gcgaggaaca gccagccgcc gctcgacacc
     4201 agcgacgcga accgccgcat cgtggtctgc cagcccgcga acgtggggac cacccactcc
     4261 gcgcagtagt acgaggtcac gagcgggtac tgcaccggct tgccgagcgg caggtcgtcg
     4321 cgcaggtcgc acgacacggg gtggatccgc gcccgcatcg cggcctcgcg ctcggcgagc
     4381 gagccgagcc ggcggcccgc ggcgtcgagc acgccgcgcg cgccgtcgag aaagatcgac
     4441 cagtcgaacg cgcccggatc gcggcggatc cagcgccgga tctcctccag ggtggagtcc
     4501 tggaagtcgg ccatgtcgag ccgctcgacc cacgggatgg cctgtgcggc gcgacacagg
     4561 accgggccgc agccgagctc gaggccggcc tcgaagtgcc ggccgatgtc ggccaggccg
     4621 cgcgcgatga accggaacat catggcttcg tcgtcgggga cgctcgggtt gccatagtag
     4681 tagtcgagat agtcccgcgc attccagtcg cgcaggatat ctccggtctc cagagtctcc
     4741 ggttcgctgt cggccatgct catgcctagg tatcaccgag ccgcggcgcc gggctagcgc
     4801 gatcggtcgt gcgcgggcgc ggctgcgact acccgccggc tgcgcgatgg cttagtctcc
     4861 ggtggttcca tgattgcgct ctactcctcc ccagggaccg cgggcatggt ggtgcactgg
     4921 ctactgctgg agctggaggt cccatacacg ctgcaacggc tcgacctggc ggcaggcgag
     4981 cacaagcagc cggcgtacct tgcggtcaac ccggccggcg tggtgccggc ggtggtgatc
     5041 gacggccagc cggtctgcga ggccgccgcg atcgcgctgc acctcgccga tgcctacccg
     5101 gcgcgcggct tcgcacccgc gatcggcaca ccggcgcgag cggcgtacta ccgctggatg
     5161 ttcttcctcg ccaacacgat gcagccggcg ttccgcgcct ggttctatcc gaccgagccg
     5221 gcgggcgagg cccacgtcga gtcagtgaag gtccaggcgc ggagccgcat cgagggcgga
     5281 tgggccctgc tcgacgccca cctcgcggcg cacgggccgt atctcctcgg cgaccagatc
     5341 acgaccccgg acttcatgct gaccatgctc atgcggtggt cgcgcaacat ggccaggacc
     5401 gccaccaact ggccgacgct cgcccggctc gccaccgcaa tgaaggcgcg gccatcgttc
     5461 aaggagctct acgcccgcga ggggctcacc gactggacgt gatcggccgc aggacaggtc
     5521 aacgccgcgc agcgcaggcc ttcggtgcgc agcgccagcc cggagtccga gccggtcgcc
     5581 ctggggacct cgaagtatgg tcacctctgt tgtcatgaag tctgtctcgg cgagc
//
LOCUS       DASVOR010000412         2178 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208457,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000412 DASVOR010000000
VERSION     DASVOR010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208457"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..673)
                     /locus_tag="VF516_08260"
     CDS             complement(86..673)
                     /locus_tag="VF516_08260"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEX8107709.1"
                     /translation="MRGLRRAGTWFALPRSCLLIRKALMHAKAKLFGHPIHQMLIVFP
                     LGLLATSLVFDIVYQVTGNVRWADISYVMIASGIIGGLLAAIFGLVDWLAIPAGTRAK
                     RIGALHGIGNVAVVALFAISWLLRYSDAGLPDAAAFVLSIAGVALALVTGWLGGELVD
                     RLAVGVDDGAHLDAPSSLSGRPARDVATGPSYVAR"
     gene            complement(693..1361)
                     /locus_tag="VF516_08265"
     CDS             complement(693..1361)
                     /locus_tag="VF516_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107710.1"
                     /translation="MKRALAIHVCADAETTGLRRHWPPAIAARDTVQRAQRICEHSGV
                     VEQRTLAGDQATAEALRDALDHAAAVLINGGLLVVTFSGHTERGDGPIETARWCLVGG
                     SLTLSQIADPLARLPPAARLVIIADTCYAAAIAHCLRGAQLAVVVAGCSEDQTMVSRA
                     RSEFVVRLEDLVCSSHPGRSIDEIRAALEDDTPDCERPVVWSNAADRWPSEVIAAPRR
                     QGER"
     gene            complement(1378..1722)
                     /locus_tag="VF516_08270"
     CDS             complement(1378..1722)
                     /locus_tag="VF516_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107711.1"
                     /translation="MKRKPLHVSLHARESSVVELTVGDRVQLDRLTPSPHGQLAERHG
                     RLLDPGVHTVALEPGRYFFKTLSDANLRVVTGGVAAGITAETKDGGSPDPPKAMGDEA
                     PGELPSLTIERA"
     gene            complement(1748..1933)
                     /locus_tag="VF516_08275"
     CDS             complement(1748..1933)
                     /locus_tag="VF516_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107712.1"
                     /translation="MHTVALPPGHYFFKTLSDANLRVVTGGVTTRTTTDDKDKWPDPL
                     PARGDEIAGEAPSLTIE"
     gene            1955..>2178
                     /locus_tag="VF516_08280"
     CDS             1955..>2178
                     /locus_tag="VF516_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107713.1"
                     /translation="MALGDVTVLRWRHTIELDAVSDRQLDDAGLAGMQVDLQWLSLHA
                     TPPVCIAIIRDDVRQLAGRFIARVHARRQCP"
BASE COUNT          331 a          746 c          770 g          331 t
ORIGIN      
        1 ggggagggaa ggagacgtgc ctggcatgcg agccaggcga gcctagccga cctggcgcgg
       61 cgatgacacg gcgatcgtcg gacggctacc gcgccacgta gctggggccg gtcgccacgt
      121 cgcgcgccgg gcggccggac agcgagctcg gggcgtcgag gtgggccccg tcatccacgc
      181 cgaccgccag ccggtcgacc agctcgccgc cgagccagcc cgtcaccagg gcgagcgcca
      241 cgccggcgat cgacaggacg aacgcggcgg cgtcgggcaa ccccgcgtcg ctgtagcgca
      301 gcagccagct gatcgcgaac agcgcgacca ccgcgacgtt gccgatgcca tgcagcgcgc
      361 cgatccgctt ggcgcgcgtc ccggcgggga tcgccagcca gtcgaccagg ccgaagatgg
      421 ccgcgagcag cccgccgatg atgccgctgg cgatcatcac gtacgagata tccgcccacc
      481 ggacgtttcc ggtgacctgg tacacgatgt cgaacaccag cgatgtcgcg agcaggccga
      541 gaggaaatac gatcagcatc tgatggatcg gatgaccgaa aagcttggct ttggcgtgca
      601 tgagtgcctt cctgatcagg agacaactcc tgggtaaagc aaaccatgta ccagcgcggc
      661 gaagtcctcg catcgctgcg gcctcgtggc aatcagcgct cgccttgccg gcgcggtgcc
      721 gcgatcacct cggacggcca gcgatccgcg gcgttgctcc ataccaccgg gcgctcgcag
      781 tccggcgtgt cgtcctccag cgcagcccgg atctcgtcga tcgaacgtcc cgggtggctc
      841 gagcagacca ggtcctcgag gcggacgacg aactcgctgc gcgcgcgaga gaccatggtc
      901 tgatcctcgc tgcagccggc gaccacgacc gcgagctggg cgccccgcag gcagtgggcg
      961 atcgccgccg cgtagcaggt atcggcgatg atcaccagcc gggcggccgg aggcagccgc
     1021 gccagcggat cggcgatctg cgacagcgtc aggctgccgc cgaccaggca ccagcgcgcg
     1081 gtctcgatcg gaccgtcgcc gcgctcggtg tgccccgaga acgtcacgac gaggagcccg
     1141 ccgttgatca ggacggccgc ggcgtgatcg agcgcgtcgc gcagcgcctc ggcggtcgcc
     1201 tggtcgcccg cgagcgttcg ctgctcgacc accccggagt gttcgcagat ccgctgcgcg
     1261 cgctgcacgg tgtcgcgcgc ggcgatcgcc ggcggccagt gccggcgcag gccggtggtc
     1321 tccgcgtcgg cgcagacatg gatcgcgagg gcgcgcttca tcgtggctgc acccgcgtca
     1381 ggcgcgctcg atcgtgaggc tcggcagctc gccgggagcc tcatctccca tcgccttggg
     1441 aggatccggc gagccgccat ctttggtttc cgcggtgatg ccagcggcaa ccccaccggt
     1501 cacgacgcgc aggttcgcgt ccgacagcgt cttgaagaag tagcggccgg gctcgagcgc
     1561 gacggtgtgc acacccgggt cgagcaggcg gccgtggcgc tcagcgagtt gcccgtgtgg
     1621 cgatggggtc agacggtcga gttggacgcg gtctccgacc gtcagctcga cgacgctgga
     1681 ctcgcgagca tgcaggctga cgtgcagtgg ttttctcttc acgcgactcc tcctcgatgg
     1741 tttgcgatca ctcgatggtg aggctcggcg cctcgccggc gatctcgtcc ccccgtgcgg
     1801 gaagaggatc gggccatttg tccttgtcat cggtggtggt ccgggtggtg accccgccgg
     1861 tcacgacgcg gaggttggcg tccgacagcg tcttgaagaa gtagtggccg ggcggcagcg
     1921 cgaccgtgtg cacccccgga tcgagcaggc ggccatggcg ctcggcgacg taaccgtgct
     1981 gcgatggcgt cacacgatcg agctggacgc ggtctccgat cgtcagctcg acgacgctgg
     2041 actcgcgggc atgcaggttg acttgcaatg gctttctctt cacgcgactc ctcctgtgtg
     2101 cattgcgatc attcgtgatg acgtccggca gctcgccggg cgcttcatcg cccgcgtcca
     2161 tgcgcgccgg caatgccc
//
LOCUS       DASVOR010000413         3019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_296195,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000413 DASVOR010000000
VERSION     DASVOR010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296195"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="VF516_08285"
     CDS             <1..185
                     /locus_tag="VF516_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX8107714.1"
                     /translation="AARGRTGQAARGGGGDGDGVRAEPVRRERPKVGRNDPCPCGSGK
                     KYKKCHGKDEEAAASE"
     gene            278..1381
                     /gene="ald"
                     /locus_tag="VF516_08290"
     CDS             278..1381
                     /gene="ald"
                     /locus_tag="VF516_08290"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEX8107715.1"
                     /translation="MRIGVPKEIKTQEFRVGMTPSGVAILVSHGHTVLLEQGAGLGSS
                     IPDEAYVKAGAKIVASREEVWGSDMVVKVKEPIAPEFALMRKDLLLFTYLHLAAAQEL
                     GKEMLSRGVNGVAYETIEPTPGDLPLLTPMSAVAGRMAVQAGAMYLERERGGKGVLLG
                     GVPGVKRGRVTVIGAGVVGSNATKIAVGLGANVTVLDVNLKTLAYLDDIYAGRISTQY
                     SDPISIERAVRESDLVIGAVLIAGARAPRLVTEQMVKDMEAGSVIVDVSIDQGGCVET
                     ARPTTHDNPTYVVHDVIHYGVANMPGAVPRTSTYALTNATIKYVVTLADHGLEKAVSA
                     VPHIITGINTYRGTVPHAAVAEALGVAHHPFRP"
     gene            1510..2208
                     /locus_tag="VF516_08295"
     CDS             1510..2208
                     /locus_tag="VF516_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8107716.1"
                     /translation="MADGRDLRMLAAREKRADFERQALVHTDALFGAAYRLTRNARDA
                     EDLVQDSLLRAYRFWDSFEQDSNCKAWLLRIVTNTFINEYQRRKRSREVLDAASAEQD
                     ATDGVLMHAEATDRQSPERTLLERSVSDDVQRALDSLPDDFRTAVVLCDVQGLSYKEI
                     ADIMQTPVGTVMSRLFRGRKLLAAALRDFAISEGYVKSERSDRPPETRDHTDETIDLE
                     KYRAARTAREGSQS"
     gene            2205..>3019
                     /locus_tag="VF516_08300"
     CDS             2205..>3019
                     /locus_tag="VF516_08300"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX8107717.1"
                     /translation="MTLCQSIETLSMALLDDELVAEEHRELELHLLDCASCRQRVEAE
                     RADFALIRKALVAPPAPALLKARIARALDAEDAAAVRSERRRWSSWLLPGSAIAAAAA
                     ALLAVFATPGAPRSQESSDVATVVKRQGLPLEVQGSSTGPWLRANFAPVEPPQFAAPG
                     IDLLGARLLPNGIANHDAALLQYLVTLGSERLTLTAVVLKDLRSDALSDGQAYQVGDQ
                     VLRVHDVNGMPAVTYIDPVDHVGYVFASEHMTADELLRLVVSTDLIERAQQSR"
BASE COUNT          482 a         1044 c         1077 g          416 t
ORIGIN      
        1 gggctgcgcg cgggcgcacg gggcaggccg cgcgcggcgg cggcggcgac ggtgacgggg
       61 tgcgtgccga gccggtgcgc cgcgagcgac ccaaggtcgg acgcaacgat ccgtgtccgt
      121 gcggctcggg caagaagtac aagaagtgcc acggcaagga cgaggaagcc gcggcaagcg
      181 aatgaggtga ccggccggtc gttcgagctg cgtctcacga tctcgtgacc ggcgacgatt
      241 gatcttgtgc gaccgaggcc cgatgctcgg cagccctatg cgcatcggcg ttcccaaaga
      301 gatcaagacg caagagtttc gcgttggcat gaccccgtcc ggcgtggcga tcctggtgtc
      361 gcacggccac accgtgctgc tcgagcaggg cgccggcctc ggcagctcga tccccgacga
      421 ggcctacgtc aaggccgggg cgaagatcgt cgcgagccgc gaggaggtct ggggcagcga
      481 catggtggtc aaggtcaagg agccgatcgc gccggagttc gcgctgatgc gcaaggacct
      541 gctgctgttc acctacctcc acctcgcggc ggcgcaggaa ctcggcaagg agatgctgtc
      601 ccgcggggtc aacggcgtgg cctacgagac gatcgagccg acgccgggcg acctgccgct
      661 gctcacgccg atgagcgcgg tcgccggccg catggcggtc caggccggcg cgatgtacct
      721 cgagcgcgag cgcggcggca agggcgtgct gctcggcggc gtgcccggcg tcaagcgcgg
      781 ccgggtaacc gtgatcggcg ccggcgtcgt cgggtccaac gcgaccaaga tcgcggtcgg
      841 cctgggcgcc aacgtcacgg tgctcgacgt caacctcaag acgctggcct acctcgacga
      901 catctacgcc ggccggatct cgacccagta ctccgatccg atcagcatcg agcgcgcggt
      961 ccgcgagagc gatctcgtga tcggcgcggt gctgatcgcc ggggcgcgcg ccccgcggct
     1021 ggtcaccgag cagatggtca aggacatgga ggccggctcg gtgatcgtcg acgtgtcgat
     1081 cgaccagggc ggctgcgtcg agaccgcgcg cccgaccacc cacgacaacc cgacctacgt
     1141 cgtgcacgac gtcatccact acggcgtggc gaacatgccc ggcgcggtgc cccggacctc
     1201 gacctacgcg ctgaccaacg ccacgatcaa gtacgtcgtc acgctggccg atcacggcct
     1261 ggagaaggcg gtgagtgcgg tgccgcacat catcacgggg atcaacacgt accgcgggac
     1321 cgtgccgcac gcggccgtgg cggaggcgct cggggtggcg caccacccgt tccggccgta
     1381 gcccgcgcgc gcggtgatcg cccgggccga tcgcgcgcgt agatcccgca acgttacatc
     1441 tcaagcacat ggaatcaccg ggatttttgc gagagtgggg ttccggggat tttcgggaac
     1501 aattgtcgta tggcggacgg tagagacctc cggatgctgg ccgcacgaga gaagcgcgca
     1561 gacttcgagc gccaggcgct cgtgcacacc gacgcgctgt tcggcgccgc gtatcgcctg
     1621 acccgcaacg cgcgcgacgc cgaggacctg gtccaggact cgctgctgcg cgcctaccgg
     1681 ttctgggact cgttcgagca ggactccaac tgcaaggcgt ggctgctccg gatcgtgacc
     1741 aacacgttca tcaacgagta ccagcgccgc aagcgcagcc gggaggtgct cgacgcggcg
     1801 agcgccgagc aggacgccac cgacggcgtg ctgatgcacg ccgaggccac cgaccgccag
     1861 tcgcccgagc ggacgctgct cgagcgcagc gtgtccgacg acgtccagcg cgcgctcgac
     1921 agcctgcccg acgacttccg gaccgcggtg gtgctgtgcg acgtccaggg cctgtcgtac
     1981 aaggagatcg cggacatcat gcagacgccg gtcggcacgg tgatgagccg gctgttccgc
     2041 ggccgcaagc tgctcgccgc cgcgctgcgc gacttcgcga tctccgaggg ctacgtcaag
     2101 tccgagaggt ccgaccggcc gcccgagacc cgcgatcaca ccgacgagac gatcgatctc
     2161 gagaagtacc gtgctgcacg tacggcacgt gagggttcgc aatcatgacg ctgtgtcaga
     2221 gcatcgaaac gctgtcgatg gcgttgctcg acgacgagct ggtcgccgag gagcaccggg
     2281 agctggagct gcacctgctc gactgcgcga gctgtcgcca gcgcgtcgag gccgagcgcg
     2341 ccgacttcgc gctgatccgc aaggcgctgg tcgcgccgcc ggcccccgcc ctgctcaagg
     2401 cgcggatcgc gcgcgcgctc gacgccgagg acgccgccgc ggtgcgcagc gagcgccgcc
     2461 ggtggtcgag ctggctgttg cccggctcgg cgatcgcggc cgcggcggcc gcgctgctgg
     2521 cggtgttcgc caccccgggc gcgccgcgct cgcaggagag ctcggacgtc gcgaccgtgg
     2581 tcaagcgcca ggggctgccg ctcgaggtcc agggctccag caccgggccg tggctgcgcg
     2641 ccaacttcgc tccggtcgag cctccccagt tcgccgcgcc cggcatcgac ctgctcggcg
     2701 cccgtctgct gcccaacggg atcgccaacc acgacgccgc gctgctccag tacctggtga
     2761 cgctcggatc ggagcggctg accctgaccg cggtcgtgct caaggacctg cggagcgacg
     2821 cgctcagcga tggccaggcc taccaggtgg gcgaccaggt gctgcgggtc cacgacgtca
     2881 acgggatgcc cgcggtgacc tacatcgacc cggtcgacca tgtcggctac gtgttcgcat
     2941 ccgagcacat gaccgccgac gagctgctcc ggctggtggt gtcgaccgac ctgatcgagc
     3001 gggcgcagca gagccggta
//
LOCUS       DASVOR010000414         7162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266635,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000414 DASVOR010000000
VERSION     DASVOR010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7162
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266635"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2059
                     /locus_tag="VF516_08305"
     CDS             <1..2059
                     /locus_tag="VF516_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107718.1"
                     /translation="MAQILDADLWLATPDAGVQVRRWWKDSAVEPIQEGNPYFARLVT
                     DLRSAKPSGMGAAGKVELAGWAFVKGSASDSTVDWPLIPGDETTTIIHLIQELRAAGV
                     DLRMLVNQFLRFDSDSLDDFPELAPILAGLWGALGPAQALLGMMTDPAGYIVGWLAVA
                     AISIALSTGWTLDRIKSFAEYSKPLVDALTAIDPTIATWTPYPAAFSDNPLVGSPPKV
                     LGHTIDDISHLGVYHQKYVVVQPASGAPVAYLGGIDINSDRVDTSIHRAVHPFHDVQA
                     RITGPAVADVIRSYEERAVFHSAPHPIATPAEGTVAATGSHIVQIARTYFAPSSPLAS
                     WPAPHGETTPVRTLEAAIAQARDYIYIEDQYFTPPDHYVQTLIDAAGNGARALVLTVP
                     FSTDQPFGGIRRADVLAALRTAWGSRFHVGTPLRRFLHQTPALTTNLGRMRLASALTA
                     AATQCELSPLSHIPPPPFWAFVGGELLLVYASVGGPSGTGADAKQAMQIARASGLPGW
                     GAVPVAHVAGEPVTAIQIPGIYVHAKVTIIDDVFVQIGSSNVNRRGFYHDGEINSFTV
                     PQHLKGAPDNPARLLRCRLMAEHMGLTPEMGQSLFADPISAIEYFARSWYQGSHRTGL
                     DFFGSLPPDTQIGTADSVFSELVKILIGSLTAAAKTDVWTLLADPTTSLDPSPSSKGP
                     GFP"
     gene            2056..>7162
                     /locus_tag="VF516_08310"
     CDS             2056..>7162
                     /locus_tag="VF516_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107719.1"
                     /translation="MKITLFDVLGIDPSVLDRASAARALWPIDLVSFTQGLEISLSGS
                     DESLAIVNMLQLGLTFQNVRPGVDPHASLGGPTGQIGFTADVAVGAFATTQPFYLRAL
                     PDHGIQLIDTDPLFPAQVWFAADGRGHEVVIERLPVKLLLKEGLVQALPSAVANVGTF
                     DKTKIDSYAYKLSTGIDATEVDCFVRLHLTVEGDLIVETAVPISFGPVRWMGLPATAV
                     YDVQLLPSPNRRDYLEWAHNDVGSFFSNPPAKGAIAFRSIDVDFSKPPFDALRDRVRN
                     GAIHLDNLELVLEDVAMPATVPGLPIPSHGTFGFRRKITDRTDIAQAYSLTGAPVQIP
                     IYGSMRQGGNGGSSLTLEIEKFFFRTGDLHALDPADQPQVQLQAALIWQRTNGDKLGA
                     TVGIDDEWTINAGMVMDLNTTPLKLTIADTTVGLVGFKFGVSVGRLTRGIEFKDSFEL
                     LGDLFLTGKATGSDSAVFKMRSLTGKQLSVVIHDLGYKLGHFSVDGLQFPDGMQLIFA
                     NTVRIIIEEMGWVEEANGTPYFSFSGGVALGFGGGNAVNPSGTGSDSQSNGFGIRVRR
                     LRFRLNDDGSQPVFKIDGVFLKLKYGTVDIEGFGYISDFTADRWAIKEWGFGVKVAFS
                     AVAMTFSIAAQFIKGSRRNLDDPTQDFDYFLAALSLGFLPAGPIGLYNIRALVAYNFA
                     PNLDSEFPDGEGMALLKWHQTHDNALTMPASRTLADWIPEKDALAFGVGCGFSLNGCG
                     SAAHLDIFIFFSKSKADTGLLIVGDLFLLRNPRPIAFVAIEYDIDKEKFGVMVGVNIG
                     LADFASGSAPAWLNNIARLSGTLYFGNKPWSFAIGQLADQSTWLALKLDWHIWLEVKF
                     MIGLCVQIVDGGPKGFGIVFTLSAGANWGIGGFVLWGTLGLIFGTWKTGSDSTGLEFW
                     VELGFKINLFWIFSFGAEIRLRITYLGKHPWYITLHAEIKIDTPWFLPDVTFTIDKTW
                     QEPMPFDTATITQCLSSASAIDSTAQAGVKLLVPGLVGALGDASFVYTFNQLGGLTGA
                     RIADPHVRDDIPVVSVDSTIAIDLAQPSSNDSAIATSTYDGTTDSGVQVVQDITVRYG
                     LQSIAVRRAPRFGPTAGTWTDFVTDAQTGFSIGGSAPEALTFTWDADTRADGKLAPKR
                     LLVNSASPYSFATAGAQNDEEAARRDPDFPCCDERTQRTTIPRPHVLEFSAIPFGART
                     PRSERFTNHGALWTWSTPAAPVVSPGDPAYPGLHVARIAPRASLLVGAADLADPAVTA
                     TLELAWQSYPGILHFEGYDGLSLIVERSVDLHTTGSTALSLSAGTTGHKISRLVIRVA
                     IDDKIDPTHQAAGPALVAAAVQLPNTGIAIYRITYISLADALRWVASKQRCGNNSGLG
                     PPGSDAKGKLAFLPNHDYEVVVTETIAVGTKDQGTRTLTLSEAFYFRTKGLPGLNACP
                     NVGDDIRRHVDATYPRQRAIPLYRQEPCVLAFENSLSSVLPIDRTPAAGDPPEKAQMF
                     PLELNVDRTVSLSGLKRLTVPSNDWIATHRANPYPPIYYVASPAFARSRVRVARSHDP
                     LVLRFEAVKAAVPACGAPKLDHASQVLLHEPIDVSGAPGPWEPTTGYRATVRQKDGPF
                     TERSGFDIYDLGAFVRQADGGASAVLWSVDADQNLVAPAAGAGRHYAACGELGWDHLQ
                     GHARIDLHGADAAGLAVGVAAGTPVPQAVLATVE"
BASE COUNT         1098 a         2627 c         2340 g         1097 t
ORIGIN      
        1 catggcgcag atcctcgacg ccgatctctg gctcgccacg cccgacgccg gtgtgcaggt
       61 caggcgctgg tggaaggaca gcgcggtcga gccgatccag gagggcaacc cgtacttcgc
      121 gcggctggtc accgatctgc gcagcgccaa gccgagcggc atgggcgccg ccggcaaggt
      181 cgagctcgcc ggctgggcgt tcgtcaaggg cagcgcgtcg gactccacgg tcgactggcc
      241 gctgatcccc ggcgacgaga ccaccacgat catccacctg atccaggagc tgcgcgccgc
      301 cggcgtcgac ctccgcatgc tggtcaacca gttcctgcgg ttcgactccg actcgctcga
      361 cgacttcccc gagctcgcac cgatcctggc cgggctgtgg ggcgcgctcg gcccggcgca
      421 agcgctgctc gggatgatga ccgatccggc gggctacatc gtcgggtggc ttgccgtcgc
      481 cgcgatctcg atcgcgctgt cgaccggatg gacgctcgat cgcatcaaga gcttcgcgga
      541 gtactccaag ccgctggtcg acgcgctcac cgcgatcgat ccgacgatcg cgacctggac
      601 gccgtatcct gccgcattct cggacaaccc gctggtcggc tcgcctccga aggtcctcgg
      661 ccacacgatc gacgacatca gccatctcgg cgtgtaccac cagaagtacg tcgtcgtgca
      721 gcccgcgagc ggcgcgccgg tcgcctacct cggcggcatc gacatcaaca gcgaccgcgt
      781 cgacaccagc atccaccgtg cggtgcatcc gttccacgac gtgcaggccc ggatcaccgg
      841 cccggccgtg gccgacgtga tccgatcgta cgaggagcgc gcggtgttcc acagcgcgcc
      901 gcacccgatc gcgacccccg ccgagggcac ggtcgccgcc accggctcgc acatcgtgca
      961 gatcgctcgg acctacttcg cgccgagcag cccgctcgcc agctggcccg cgccgcatgg
     1021 cgagaccacg ccggtgcgca cgctcgaggc ggcgattgca caggcgcgcg actacatcta
     1081 catcgaagat cagtacttca caccgcccga ccattacgtg cagacgctga tcgacgccgc
     1141 cggcaacggc gcgcgcgcgc tggtcctgac cgtgccgttc tcgaccgatc agccgttcgg
     1201 cggtatccgc cgcgccgacg tgctggcggc gctgcgcacc gcgtggggat cgcggttcca
     1261 cgtcggcacg ccgctgcgcc ggttccttca ccagacaccg gcgctcacca ccaacctcgg
     1321 ccggatgcgg ctcgcgtcgg cgctcaccgc ggccgcgacg cagtgcgagc tgtcgccgct
     1381 gtcgcacatc cccccgccgc cgttctgggc attcgtcggc ggcgagctcc tgctggtcta
     1441 cgcgtcggtc ggcggaccct cggggaccgg tgccgacgcc aagcaggcca tgcagatcgc
     1501 ccgggcgagc gggctcccgg gctggggcgc ggttccggtc gcccatgtcg ccggcgagcc
     1561 ggtcaccgcg atccagattc ccggcatcta cgtccacgcc aaggtcacga tcatcgacga
     1621 cgtcttcgtg cagatcggct cgtcgaacgt caaccgccgc ggcttctacc acgacggcga
     1681 gatcaactcg ttcaccgtcc cgcagcacct caagggcgcc cccgacaacc cggcgcgcct
     1741 gctgcgctgc cggctgatgg ccgagcacat ggggctgacg ccggagatgg gccagtcgct
     1801 gttcgccgac ccgatctcgg cgatcgagta cttcgcccgg tcgtggtacc agggctcgca
     1861 ccgcaccggg ctggacttct tcggctcgct gccacccgac acccagatcg gcaccgccga
     1921 ctcggtgttc agcgagctgg tgaagatcct gatcggcagc ctgacagcag cggccaagac
     1981 cgacgtctgg acgctgctgg ccgaccccac gacgtcgctc gatccgtcgc ccagctcgaa
     2041 gggacccggg ttcccatgaa gatcacgctc ttcgatgtgc tcgggatcga tcccagcgtg
     2101 ctggaccggg cgagcgccgc cagggcgctg tggccgatcg acctcgtgtc gttcacccag
     2161 ggcctcgaga tctcgctgtc gggctccgac gagtcgctcg cgatcgtcaa catgctgcag
     2221 ctcggcctga cgttccagaa cgtgcggccg ggcgtcgatc cgcacgccag cctcggcggt
     2281 cccaccggcc agatcggctt caccgcggac gtcgcggtcg gcgcgttcgc caccacgcag
     2341 ccgttctacc tgcgcgccct gcccgatcac ggcatccagc tgatcgatac cgatccgctg
     2401 ttcccggcgc aggtctggtt cgccgccgac ggacgcggcc acgaggtcgt gatcgagcgc
     2461 ctgccggtca agctgctgct caaggagggg ctggtccagg ccctgccgtc ggccgtcgcc
     2521 aatgtcggga cgttcgacaa gaccaagatc gacagctacg cctacaagct gagcaccggc
     2581 atcgacgcga ccgaggtcga ctgcttcgtg cggctacacc tcaccgtcga gggcgatctg
     2641 atcgtcgaga ccgcggtccc gatctcgttc ggcccggtgc gctggatggg cctgccggcg
     2701 accgcggtgt acgacgtcca gctgctgccg tcgcccaacc gccgcgacta cctcgagtgg
     2761 gcgcacaacg acgtcggctc gttcttctcg aacccgccgg ccaagggcgc gatcgcgttc
     2821 cgcagcatcg acgtcgactt ctcgaagccg ccgttcgatg ccctgcgcga ccgcgtccgc
     2881 aacggcgcga tccacctcga caacctcgag ctcgtgctcg aggacgtcgc gatgccggcg
     2941 accgtgccgg gcctgccgat cccgtcgcac ggcacgttcg ggttccgccg caagatcacc
     3001 gatcggaccg acatcgcaca ggcgtactcg ctgacgggcg cgccggtcca gatcccgatc
     3061 tacggctcga tgcgccaggg cggcaacggc ggctcctcgc tgaccctcga gatcgagaag
     3121 ttcttcttcc gcaccggcga cctccacgcg ctcgatccgg ccgaccagcc gcaggtccag
     3181 ctccaggccg cgctgatctg gcagcgcacc aacggcgaca agctcggggc cacggtcggc
     3241 atcgacgacg agtggacgat caacgccggc atggtcatgg acctcaacac cacgccgctc
     3301 aagctcacga tcgccgatac cacggtcggc ctcgtcggct tcaagttcgg cgtgtcggtc
     3361 ggccggctga cccggggcat cgagttcaag gacagcttcg agctgctcgg cgatctgttc
     3421 ctcaccggca aggcgaccgg ctcggacagc gcggtgttca agatgcgctc gctgaccggc
     3481 aagcagctca gcgtcgtgat ccacgacctc ggctacaagc tcgggcactt ctcggtcgac
     3541 ggcctgcagt tccccgacgg gatgcagctg atcttcgcca acacggtgcg gatcatcatc
     3601 gaggagatgg gctgggtcga ggaggccaac ggcacgccgt acttcagctt cagcggcggc
     3661 gtcgcgctcg gcttcggcgg cggcaacgcg gtcaacccga gcggcaccgg cagcgacagc
     3721 cagagcaacg gcttcggcat ccgggtgcgt cgcctccggt tccggctcaa cgacgacggg
     3781 tcgcagccgg tgttcaagat cgacggcgtc ttcctcaagc tcaagtacgg cacggtcgac
     3841 atcgagggct tcggctacat cagtgacttc accgccgacc gctgggcgat caaggagtgg
     3901 gggttcggcg tcaaggtcgc gttcagcgcg gtcgcgatga cgttctcgat cgcggcgcag
     3961 ttcatcaagg gcagccggcg caacctcgac gacccgaccc aggacttcga ctacttcctc
     4021 gccgccctga gcctgggctt cctgccggcg gggccgatcg gcctctacaa catccgcgcg
     4081 ctggtcgcct ataacttcgc ccccaacctc gactcggagt tccccgacgg cgagggcatg
     4141 gcgctgctca agtggcacca gacccacgac aacgcactga ccatgccggc cagccgcacg
     4201 ctggccgact ggatcccgga gaaggatgcg ctcgcgttcg gcgtcggctg cgggttctcg
     4261 ctcaacggct gcggctcggc ggcccacctc gacatcttca tcttcttctc caagagcaag
     4321 gccgacaccg gcctgctgat cgtcggcgac ctgttcctgc tcaggaaccc caggccgatc
     4381 gcgttcgtcg cgatcgagta cgacatcgac aaggagaagt tcggcgtgat ggtcggtgtc
     4441 aacatcggcc tggccgactt cgcgtcgggc agcgcgccgg cgtggctcaa caacatcgcg
     4501 cggctcagcg gcacgctgta cttcggcaac aagccgtggt cgttcgcgat cggccagctc
     4561 gccgatcaga gcacgtggct ggcgctcaag ctcgactggc acatctggct cgaggtcaag
     4621 ttcatgatcg gcctgtgcgt ccagatcgtg gacggcgggc cgaagggctt cggcatcgtg
     4681 ttcacgctga gcgccggcgc gaactggggg atcgggggct tcgtcctgtg ggggacgctc
     4741 ggcctgatct tcgggacgtg gaagaccggc tcggactcga ccggcctcga gttctgggtc
     4801 gagctcggct tcaagatcaa cctgttctgg atcttcagct tcggcgccga gatccggctg
     4861 cgcatcacct acctcggcaa gcacccctgg tacatcacgc tgcacgccga gatcaagatc
     4921 gacactccgt ggttcctgcc cgacgtcacg ttcacgatcg acaagacgtg gcaggagccg
     4981 atgccgttcg acacggcgac gatcacgcag tgtctgtcga gcgcgtcggc gatcgactcg
     5041 accgcgcagg ccggcgtcaa gctgctcgtg cccggcctgg tcggcgcgct gggcgacgcc
     5101 tcgttcgtct acacgttcaa ccagctcggt ggcttgaccg gcgcgcggat cgccgatccg
     5161 cacgtgcgtg acgacatccc ggtggtgtcg gtcgactcga cgatcgcgat cgacctcgcg
     5221 cagccgagct cgaatgactc ggcgatcgcc acctcgacct acgacggcac gaccgacagc
     5281 ggcgtgcagg tcgtgcagga catcacggtg cgctacggcc tgcagtcgat cgcggtgcgg
     5341 cgcgcgccca ggttcggtcc gaccgccggg acgtggaccg acttcgtcac cgacgcgcag
     5401 accgggttct cgatcggcgg ctcggccccc gaggcgctca cgttcacctg ggacgccgac
     5461 acccgcgccg acggcaagct cgcgcccaag cgcctgctcg tcaacagcgc gtcgccgtac
     5521 tcgttcgcga ccgcgggcgc gcagaacgac gaggaggcgg cgcgccgcga tccggacttc
     5581 ccgtgctgcg acgagcgcac gcagcgcacc acgatcccca ggccgcacgt gctggagttc
     5641 agcgcgatcc cgttcggcgc gcgcacgccg cgcagcgagc ggttcaccaa ccacggcgcg
     5701 ctgtggacgt ggtcgacgcc ggcggcgccc gtggtgtcac ccggcgatcc ggcgtacccc
     5761 ggactgcacg tcgcgcggat cgcgccgagg gcctcgctgc tggtcggcgc cgccgatctc
     5821 gccgacccgg cggtcaccgc gacgctcgag ctcgcctggc agagctaccc cggcatcctg
     5881 cacttcgagg gctacgacgg gctgtcgctg atcgtcgagc gctcggtgga cctccacacc
     5941 accggctcga cggcgctgag cctgtcggcc gggacgaccg gccacaagat ctcccgcctg
     6001 gtgatccgcg tcgcgatcga cgacaagatc gatccgacgc accaggcggc cggcccggcg
     6061 ctggtcgccg ccgcggtcca gctgccgaac accggcatcg cgatctaccg catcacctat
     6121 atctcgctcg ccgacgcgct gcgctgggtc gcgagcaagc agcgctgcgg caacaacagc
     6181 gggcttggcc cgccgggctc cgatgccaag ggcaagctcg ccttcctgcc caaccacgac
     6241 tacgaggttg tcgtcaccga gacgatcgcc gtcggcacca aggaccaggg cacgcggacg
     6301 ctcacgctgt cggaggcgtt ctacttccgc accaagggcc tgcccgggct caacgcgtgc
     6361 cccaacgtcg gcgacgacat ccgccgccat gtcgacgcca cgtatccgcg ccagcgcgcg
     6421 atcccgctgt accgccagga gccctgcgtc ctggcgttcg agaactcgct gagctccgtg
     6481 ctgccgatcg accgcacgcc ggccgccggc gatccgcccg agaaggcgca gatgttcccg
     6541 ctcgagctca acgtcgatcg cacggtgtcg ctgagcgggc tcaagcggct caccgtgccg
     6601 agcaacgact ggatcgccac gcaccgcgcc aacccgtacc cgccgatcta ttacgtcgcg
     6661 tcgccggcgt tcgcccggtc cagggtgcgc gtcgcgcgct cgcatgatcc gctggtgctg
     6721 cgcttcgagg ccgtcaaggc agcggtgccg gcctgcggcg cgcccaagct cgaccacgca
     6781 agtcaggtgc tgctacacga gccaatcgac gtcagcggcg cgccggggcc gtgggagccg
     6841 acgaccggct atcgcgcgac cgtgcggcag aaggacgggc cgttcaccga gcgctcgggc
     6901 ttcgacatct acgatctcgg cgcgttcgtg cgccaggccg acggcggcgc ctccgcggtg
     6961 ctgtggtcgg tcgatgccga tcagaacctc gtcgcaccgg ccgctggcgc gggccggcac
     7021 tacgccgcgt gcggtgagct gggctgggat cacctgcagg ggcacgcgcg gatcgatctg
     7081 cacggcgccg atgccgcggg cctcgcggtc ggcgtcgctg ccggcacgcc ggtgccgcag
     7141 gccgtgctgg cgaccgtcga gg
//
LOCUS       DASVOR010000415         3457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_3970,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000415 DASVOR010000000
VERSION     DASVOR010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3457
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3970"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(315..1709)
                     /locus_tag="VF516_08315"
     CDS             complement(315..1709)
                     /locus_tag="VF516_08315"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8107720.1"
                     /translation="MKRAAVVIATLVGCHPAPTPKPPTEAAIDQPPPPPPPTGPVAPA
                     DPKPIVDGDVVDAQTHGIRVLIQRAPGVEEIATGLYIAGGSLGWDARTAGIEQLALSV
                     AANGGTRQLDKDRFTRKLSDLGSQIGAGSNEDYSQMTAWSLKSTWDDTFALLVDAFRD
                     PALPASEIEIQRQRQLSDLKQEADSLDARLDLVTHQGMFKGHPYENRAIGTIETVSSF
                     TAPQLAAHLAKLRESQRLLVVVVGDVDADHVIATVGKALGDLPAGSFKPNALPAVPAV
                     AKGEVTVVADKLPTNYIQAVFIGPRWTDPDFIVARVAMRWLGTRGFEEVRTKRNLSQA
                     PAAFFNWNTGCPTGALSVTAADPRMTMKVMLDEAKQLRDTKLSEHDLVAMKAELLTGA
                     YLASEAPADQAAMLAASQLRGGDWHFVRTFPDRVKAVTADQVQAWAQKAITHLHTFVI
                     GDGQKIDKQLLETF"
     gene            complement(1706..2974)
                     /locus_tag="VF516_08320"
     CDS             complement(1706..2974)
                     /locus_tag="VF516_08320"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8107721.1"
                     /translation="MVVESHSVPLVTIEITVHNGSMTEPVDYNGLSHLYEHMFFKANK
                     ALPSQEAFLRRTQELGLVWYGATSQERVNYSFTGTKDHTEELMQLMHDALVSPLFDPK
                     ELDRERVVVTGEIDRNEASPLYHLNKAINDRVFWKYPSRRYPLGNRATVLKATVAQLQ
                     TIQRRYYVPNNSALVVTGDVTAAEIFAEADRLYADWPKGEDPFIKFPLVKHPPIKKTE
                     VAVVEQPVHMVTGQMTWQGPSVAGDEIPMTYAADALSSTLSEPWSRWTKALVESGACV
                     SVSISWATQVNTGPIKVSFQATPDKVDTCITAIRAELVKMESPDYLTQEELHRGAHTY
                     EIGAVHERERPSQLSHSLTFAFCYGGLDYYLLSYPDNLYKVTPADVARYVTTYIKDRP
                     FVFGIMVSSEMRKAGLDQKHFDGLIKGRVK"
     gene            complement(3213..>3457)
                     /locus_tag="VF516_08325"
     CDS             complement(3213..>3457)
                     /locus_tag="VF516_08325"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8107722.1"
                     /translation="CTAGVFDGFRFDALGNLWTSAGDGVHCYAPDGALLGKIAVPEIV
                     ANVCFGGPRRNRLFICATTSLYAIYLAVRGAVRPAP"
BASE COUNT          532 a         1111 c         1226 g          588 t
ORIGIN      
        1 gcgtagcagt ggacgccgtc gcccgcgctg gtccacaggt tgccgagcgc gtcgaaccgg
       61 aagccgtcga acacgcccgc ggtgcaggtc gcgaacaccc cgccgcccga cagcgtccgg
      121 ccgtcggacg ccaccgcgaa ccggcggatg tgggccggcc cgccgggtac gtggctcacg
      181 ccggtgtcgg cgatgtagag caggcgctcg tcgggcgcgc gggcgctgcg cgcgcatcgc
      241 agcgcgcgta catcaacttc acgaccgacg cgctcctaca cgatcacgac cgcgccgagc
      301 gacgcctggc tggtctagaa tgtttcgaga agctgcttgt cgatcttctg gccgtcgccg
      361 atcacgaacg tgtgcaggtg cgtgatggcc ttttgcgccc acgcctgcac ctgatccgcg
      421 gtcaccgcct tcacgcgatc cgggaacgtc cgcacgaagt gccagtcgcc gccgcgcagc
      481 tgcgacgctg cgagcatcgc cgcctgatcc gcgggcgcct cgctggcgag atatgcgccg
      541 gtgagcagct cggccttcat cgcgacgaga tcgtgctcgc tcagcttcgt gtcgcgaagc
      601 tgcttggcct cgtcgagcat gaccttcatc gtcatgcgcg gatcggccgc ggtgacggag
      661 agcgcgcccg tcgggcagcc ggtgttccag ttgaagaagg cggccggcgc ctgcgacagg
      721 ttgcgctttg tgcggacctc ctcgaacccg cgcgtgccga gccagcgcat tgcgacgcga
      781 gcgacgatga agtcaggatc ggtccagcgt ggaccgatga acacggcctg gatgtagttc
      841 gtcggcagct tgtcggcgac gacggtgacc tcgcccttcg cgacggcggg cacggcgggc
      901 agcgcgttcg gcttgaagct cccggccggc aggtcgccga gcgccttgcc gactgtcgcg
      961 atcacgtggt cggcatcgac atcgccgacg accacgacga gcaggcgctg gctctcgcgc
     1021 agcttcgcga ggtgcgcggc gagttgcggc gcggtgaacg acgacacggt ctcgatcgtc
     1081 ccgatcgcgc ggttctcgta cgggtggccc ttgaacatgc cctgatgggt gacgaggtcg
     1141 agccgcgcgt cgaggctgtc ggcttcctgc ttcaagtccg acagctggcg ctggcgctgg
     1201 atctcgatct cgctcgccgg cagcgcgggg tcgcgaaacg cgtcgacgag cagcgcgaac
     1261 gtatcgtccc acgtcgactt cagcgaccac gcggtcatct gcgagtagtc ctcgttcgag
     1321 ccggcgccga tctggctgcc gaggtccgac agcttccggg tgaagcgatc cttgtcgagc
     1381 tgcctggtgc cgccgttcgc ggcgacggac agcgcgagct gctcgatgcc ggccgtcctg
     1441 gcgtcccagc cgagcgagcc accggcgatg tacaggccgg tcgcgatctc ctcgacgccg
     1501 ggcgcgcgct ggatcagcac gcggataccg tgggtctgcg cgtcgacgac gtcgccgtcg
     1561 acgatgggct tcggatcggc gggcgcgacg gggccggtcg gtggcggagg tggcggcggc
     1621 tggtcgatcg ccgcttccgt cggcggcttc ggtgtgggcg ccgggtgaca gccgacgagc
     1681 gtggcgatga ccacggcagc gcgcttcatt tcacacgtcc cttgatcagc ccgtcgaagt
     1741 gcttctgatc gaggccggcc ttgcgcatct ccgacgatac catgatgccg aacacgaacg
     1801 gcctgtcctt gatgtaggtc gtgacgtagc gagcgacgtc ggccggcgtg accttgtaca
     1861 ggttgtcggg gtacgagaga agatagtagt cgagcccgcc gtagcagaac gcgaacgtca
     1921 gcgagtgaga gagctggctc ggccgctcgc gctcgtggac cgcgccgatc tcgtatgtgt
     1981 gcgcgccgcg atggagctct tcctgcgtca ggtagtcggg tgactccatc ttgacgagct
     2041 cggcgcggat cgcggtgatg caggtgtcga ccttgtcggg ggtggcctgg aaggacacct
     2101 tgatcgggcc ggtgttgacc tgcgtggccc aggagatgct gacggacacg caggcgccgg
     2161 actcgacgag tgccttggtc caccgtgacc acggctcgga cagtgtggag gacagcgcat
     2221 cggccgcgta cgtcatcggg atctcgtcgc cggcgaccga cgggccctgc cacgtcatct
     2281 ggccggtgac catgtgcacc ggttgctcga cgacggcgac ctcggtcttc ttgatcggcg
     2341 ggtgcttgac cagcgggaac ttgatgaacg ggtcctcgcc cttcggccag tcggcgtaga
     2401 gcctgtcagc ctcggcgaag atctcggcgg cggtgacgtc tccggtcacg acgagcgccg
     2461 agttgttcgg cacgtaatat cggcgctgga tcgtctgcag ctgtgcgacg gtcgccttca
     2521 gcacagtggc gcgattgccg agcggatacc tgcgcgaggg gtacttccaa aacacccgat
     2581 cgttgatggc cttgttgagg tggtagagcg gactggcctc gttgcgatcg atctcgccgg
     2641 tcacgacgac gcgctcgcga tcaagctcct tcggatcgaa cagcggcgac acgagcgcgt
     2701 cgtgcatgag ctgcatgagc tcttcggtgt gatccttcgt gccggtgaag gagtagttca
     2761 ctcgctcctg gctcgtcgcg ccgtaccaca ccaggccgag ttcctgggtc cggcgcagga
     2821 acgcttcctg gctggggagc gccttgttcg ccttgaagaa catgtgctcg tagagatgcg
     2881 acaggccgtt gtagtcgacc ggctccgtca tcgagccgtt gtgaaccgtg atctcgatcg
     2941 tgacgagcgg gacggaatgg ctctcgacga ccatcacggt cagtccatcg ggacacgtat
     3001 gggtctgcac cttcggcgga tgggtcgagc gccccgcgac gacgccaagc acgaaaggaa
     3061 gaaggatcca catgatgcgg ggcagtctat atcaattcta ccggatcaca ccgatccgcc
     3121 gcggatttcc gccccgacgg cgggcaccgc gaacgacgcg tgggacatcg gcgatctggg
     3181 ccggggtcga cgagcggcga tcggcgagcg cctcagggcg ccgggcgcac cgcgccgcgg
     3241 accgcgaggt agatcgcgta cagcgaggtg gtcgcgcaga tgaacagccg gttgcgcctg
     3301 gggccgccga agcagacgtt ggcgacgatc tcgggcacgg cgatcttgcc gagcagcgcg
     3361 ccgtcggggg cgtagcagtg gacgccgtcg cccgcgctgg tccacaggtt gccgagcgcg
     3421 tcgaaccgga agccgtcgaa cacgcccgcg gtgcagg
//
LOCUS       DASVOR010000416         3767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_62297,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000416 DASVOR010000000
VERSION     DASVOR010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62297"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3751)
                     /locus_tag="VF516_08330"
     CDS             complement(<1..3751)
                     /locus_tag="VF516_08330"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX8107723.1"
                     /translation="MEAHGTGTSLGDPIEVRALGAVFGPGRSPEHPLVLGSVKTNIGH
                     LESAAGVAGLIKVVLALQHRAIPPHLHLRELNPDVAQEPMPKLIPSSTVPWTGDGPRI
                     AGVSAFGLSGTIAHMVVEEAPAAPVTEVEPDHALVLPISAHSPEALHELATAYHDLMS
                     RPDAPAVRDLCYSAAVRRTHHDHRLAVAGETHAELAERLSAFLADKPRSGRASGVRAA
                     STAHKLVFVFPGQGGQWPGMAQDLLAREPVFAEAIAACEAALRPHVDWSLRELLAASP
                     APPLLERIDVIQPALFSLQVALAALWRSFGIVPQAVVGHSMGEIAAAHVAGALSLSDA
                     ARIIARRSRLMRTISGRGAMAVLELPLADAERALAGREDRLAIAASNGPTTTVVSGDP
                     AALAELLDELGQREVFCRPVKVDVASHSPQVDVLRPALHAALAGLAPRSTAIPFHSTV
                     TGAPADGYELDAAYWESNLRRPVLFAPVVEQLIAQGHDLFVELSPHPVLGGALQQLLH
                     GHGGEGGVFTSLRRDEPGRTILLGALGGLYAHGYPVDWSRLFASPGHVVPLPSYPWQR
                     ERFWIETAGPAPGRRTHESRAASYASPLLGDALHSPGLDEAIFESEISLDALPFLTDH
                     RIDHEVVVPGASHVVRLLCAAEQLHGAAACALDGVSFPAALVLPEAEVRTAQLIVDPD
                     GAFRTVTAPRGSEADEAAWVVHAAGRLRKASDAPAAAESLDDIRARCRDHKTGAEFYD
                     EMSRAGYHLGPSFRWIDSICYRDGEALGQMRTPRNPDEAERYPVFPGLLDSCFQLLAA
                     ASRASSPVAMAETGALYVPVAVTHMHFHRRIGAAPVLCHVVLGERTGDEIAAEIRLRD
                     EQGTIAKVGFRGKRVDRERFVRGSAAQRDLFYRTDWQRADAPSRPARSPGRYLVLADR
                     GDVAARLTALLDARGATGTLVTAAVLRDAAAVRCTVDDALRGDPPLASIVHLGSLDAP
                     PYATTSLAALDAARTTGCDSILHVVQALVHAAPRHAPRLHLVTAGAQAVGDAASVSPA
                     QTAVWGLARVIAHEHPELRCVCADLSLAPSAAEIAALADEIAAGDREDQVAFRDDARH
                     VARLVSHRLGEGTAPAKQPAATVLAGDQPYRLEIDAPGVLEDLVLRAVPRPAPRADEV
                     EIEVRAAGINFVDVLSALGIRPDHTDGRTQLGGECSGIVTRVGERVTDVKPGDAVIAA
                     LVPDAFSSFVRVPSRFVVAKPAHIGFEDAATLPITFMTAYWAMHHK"
BASE COUNT          537 a         1352 c         1408 g          470 t
ORIGIN      
        1 ccttgtggtg catcgcccag tacgcggtca tgaacgtgat ggggagtgtc gcggcgtcct
       61 cgaagccgat gtgcgcgggc ttggccacga cgaaccggct cggcacccgg acgaacgatg
      121 agaaggcgtc cggcacgagc gcggcgatca ccgcgtcccc gggcttgacg tcggtcacgc
      181 gctcgccgac ccgggtgacg atgccggagc attcgccgcc gagctgggtt ctgccgtcgg
      241 tgtgatcggg ccggatgccg agcgccgaca gcacatcgac gaagttgatc ccggcggcgc
      301 ggacctcgat ctcgacctcg tccgcccgcg gggccggacg cggaacggcg cgcagcacca
      361 gatcttcgag cacgccgggc gcatcgatct ccagcctgta cggctgatcg ccggccagga
      421 ccgtggccgc tggctgcttc gccggcgctg tcccctcacc gagccggtgc gagacgaggc
      481 gcgcgacgtg gcgtgcgtca tcgcggaacg cgacctggtc ctcgcgatcg ccggcggcga
      541 tctcgtcggc cagcgcggcg atctcggccg ccgaaggcgc gaggctgagg tcggcgcaca
      601 cgcagcgcag ctcgggatgc tcgtgcgcga tcacgcgcgc gaggccccac accgcggtct
      661 gcgcgggcga caccgacgct gcgtccccga ccgcctgggc tcctgcggtg acgaggtgga
      721 gccgcggggc atggcgcggc gccgcgtgga cgagcgcctg gacgacgtgc aggatcgagt
      781 cgcagccggt cgtccgcgcg gcatcgagcg ccgcgaggct ggtggtcgca tacggcggtg
      841 catcgaggct gccgaggtgc acgatgctcg cgagcggcgg atcgccgcgg agcgcgtcgt
      901 ccaccgtgca gcgaaccgcg gctgcgtcac gcagcaccgc ggccgtcacc agggtaccgg
      961 tggctccacg tgcatcgagc agcgcggtga gccgggcggc cacgtcgccg cgatcggcca
     1021 gcacgaggta tcgccccggc gagcgcgccg gtcgcgacgg ggcgtcggcg cgctgccagt
     1081 ccgtccggta gaacaggtcg cgctgggccg cagacccgcg cacgaaccgc tcgcgatcga
     1141 cgcgcttgcc gcggaagcca accttggcga tcgtgccttg ctcgtcgcgc aggcggatct
     1201 cggcggcgat ctcgtcgccg gtgcgctcgc cgagcaccac atggcacaac accggcgccg
     1261 cgccgatccg gcgatgaaag tgcatgtgtg tgaccgccac cggcacgtac agcgcgccgg
     1321 tctcggccat cgcgaccggc gagctcgctc gcgacgcggc agcgagcagc tggaaacagg
     1381 aatcgagcag cccggggaac accggatagc gctccgcctc gtcggggttg cgcggtgtgc
     1441 gcatctgccc cagcgcctcg ccgtcgcgat agcagatcga atcgatccac cggaagctcg
     1501 ggcccaggtg atagcccgcg cggctcatct cgtcgtagaa ctcggcgccg gtcttgtggt
     1561 cccggcagcg ggcgcggatg tcatcgagcg attcggcggc cgcgggcgcg tcgcttgcct
     1621 tgcgcaggcg gccggctgcg tggacgaccc acgctgcctc gtcggcctcg ctacctcggg
     1681 gcgccgtgac ggtgcgaaac gcgccgtcgg ggtccacgat cagctgtgcg gtgcggacct
     1741 ccgcctcggg caggaccagc gccgccggga acgagacgcc atccagcgca cacgcggcag
     1801 cgccgtgaag ctgctccgcc gcgcacagca ggcggaccac gtgcgaggcg cctggcacga
     1861 ccacctcgtg atcgatgcgg tggtcggtga ggaacggcag ggcgtcgagg ctgatctcgg
     1921 actcgaagat cgcctcgtcg agccccggag aatgcagcgc atcgccgagc agcggtgaag
     1981 cgtacgacgc ggcccggctc tcgtgcgtgc ggcggcccgg cgccggccct gccgtttcga
     2041 tccagaaccg ttcacgctgc cagggatacg acggcagcgg caccacatgt ccgggagagg
     2101 cgaacagccg cgaccagtcc accgggtagc cgtgcgcgta caacccgccc agcgcgccga
     2161 gcaggatcgt gcggcccggc tcgtcgcggc gcagcgacgt gaacacgccg ccctcgccgc
     2221 cgtggccgtg cagcagctgc tgcagcgcgc cgccgagtac cgggtgcggc gacagctcga
     2281 cgaacaggtc atggccctgc gcgatcagct gctcgacgac cggcgcgaac agcaccggcc
     2341 ggcgcaggtt gctctcccag tacgcggcgt cgagctcgta accgtcggcg ggcgcgccgg
     2401 tcaccgtcga gtggaacggg atggcagtgg accgcggcgc caggccggcg agcgccgcgt
     2461 ggagcgccgg gcgcagcacg tcgacctgcg ggctgtgcga ggcgacgtcg accttgaccg
     2521 gccggcagaa cacctcacgc tggccgagct cgtcgagcag ctccgccagc gcggcaggat
     2581 cgcccgacac caccgtggtc gtcggtccgt tgctggcggc gatggcaagc cgatcctcgc
     2641 ggccggccag cgcgcgctcg gcgtcggcca gcggcagctc cagcaccgcc atcgcgccgc
     2701 ggccgctgat cgtgcgcatg agccggctcc ggcgagcgat gatgcgcgcc gcgtcgctga
     2761 gcgacagcgc gccggcgacg tgcgcggccg cgatctcgcc catgctgtgg ccgaccaccg
     2821 cctgcggcac gatgccaaac gatcgccaca gcgcggcgag cgcgacctgc agcgagaaca
     2881 gcgccggctg gatgacgtcg atccgctcca gcagcggcgg cgcaggcgag gcggcgagca
     2941 gctcgcgcag cgaccagtcg acgtggggcc ggagcgcggc ctcgcacgcc gcgatcgcct
     3001 cggcgaacac cggctcgcgc gcgagcaggt cctgcgccat gccaggccac tggccgccct
     3061 gccccgggaa cacgaacacc agcttgtgcg cggtggacgc ggcgcggact ccgctggccc
     3121 gtccggagcg cggcttgtcg gcgaggaacg cggacagccg ctcggcgagc tcggcgtggg
     3181 tctcaccggc caccgcgagc cggtgatcgt gatgggtgcg gcgaaccgcg gccgagtagc
     3241 acaggtcgcg gacggccgga gcgtcggggc gagacatgag gtcgtggtac gcggtggcga
     3301 gctcgtgcag cgcctcgggg ctgtgcgccg agatcgggag cacgagggcg tggtcgggtt
     3361 cgacctcggt gaccggcgcc gcgggcgcct cctccaccac catgtgcgcg atcgtcccgc
     3421 tcaggccgaa tgcgctgacg ccggcgatgc gcgggccgtc gccggtccat ggcaccgtcg
     3481 acgacgggat cagcttgggc atcggctcct gcgccacgtc ggggttgagc tcgcgcaggt
     3541 ggaggtgcgg cgggatcgcg cggtgctgca gcgccagaac gaccttgatc agcccggcga
     3601 cgcccgcggc cgactcgagg tggccgatgt tggtcttcac cgatcccagc acgagcggat
     3661 gctccgggct gcggccgggg ccgaacaccg caccgagcgc gcgcacctcg atcggatcgc
     3721 cgagcgacgt gccggtcccg tgcgcctcca cgtagctcac gtcaccg
//
LOCUS       DASVOR010000417         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000417 DASVOR010000000
VERSION     DASVOR010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VF516_08335"
     CDS             <1..1089
                     /locus_tag="VF516_08335"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase domain-containing protein"
                     /protein_id="HEX8107724.1"
                     /translation="RYEWIDTLFALPEAVMYLTLVDWHDRQVAPVDYHKLFTDIRTAI
                     DEAHRDDTLKSVIKADLGAFIVKDPLLGETLHKFRSSGKKLFLLTNSLYDYTASVMSY
                     LLDGERKAYPSWRNYFDIVMVAAGKPAFFNELRPFVQIDPATGAPVGNGNGESIKHLA
                     RDKVYQAGNVIAFEQLTGIKGEQVLYIGDHIYGDILRLRKQHMWRTAMVLQELEREIS
                     VSDRLEAQIKDLDLLDRRHRNLESEIDYQSLRLKKIQRLLEDAATAPQLRIRLDDARR
                     LTRESLDALRDRAQLMDAEVDSLEDRIDHAYNPHWGSCLREGNENSRFGEQVNDYADL
                     YTSRVSNFGPYSPLRYFRAPRRPMPHEI"
     gene            1132..>1615
                     /locus_tag="VF516_08340"
     CDS             1132..>1615
                     /locus_tag="VF516_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107725.1"
                     /translation="MPPSPPPPPARALASIQVAIAVVGVAVLAWRASHYWPFVADDAL
                     ISLRYARRLVAGHGLTWTDGERVEGYSNLLWVAACAGLHALGLDLITAARALGLAGHA
                     AVICAIVRIAVVRSLGEALALLAIVLAFALTGPIDAWAIGGLEQPLLVALLAWAVALG
                     L"
BASE COUNT          242 a          571 c          556 g          246 t
ORIGIN      
        1 cgctacgagt ggatcgacac gctgttcgcg ctgcccgagg cggtgatgta cctgacgctg
       61 gtcgactggc acgatcgcca ggtcgcgccg gtcgactacc acaagctgtt caccgacatc
      121 cggaccgcga tcgacgaggc ccaccgcgac gacaccctga agagcgtgat caaggccgac
      181 ctcggcgcgt tcatcgtcaa ggacccgctg ctcggcgaga ccctgcacaa gttccgcagc
      241 tcgggcaaga agctgttcct gctcaccaac tcgctctacg actacaccgc gagcgtgatg
      301 agctatctgc tcgacggcga gcgcaaggcc tacccgtcgt ggcgcaacta cttcgacatc
      361 gtgatggtcg ccgccggaaa gccggcgttc ttcaacgagc tgcggccgtt cgtccagatc
      421 gaccccgcga ccggcgcgcc ggtcggaaac ggcaacggcg agtcgatcaa gcacctggcg
      481 cgcgacaagg tctaccaggc cggcaacgtg atcgcgttcg agcagctcac cgggatcaag
      541 ggcgagcagg tgctctacat cggcgaccac atctacggtg acatcctgcg gctgcgcaag
      601 cagcacatgt ggcgcaccgc gatggtgctg caggagctcg agcgcgagat ctcggtcagc
      661 gaccggctcg aggcccagat caaggacctc gatctgctcg accgccgcca ccgcaacctc
      721 gagtccgaga tcgattacca gtcgctgcgg ctcaagaaga tccagcggct gctcgaggac
      781 gcggccaccg cgccccagct gcggatccgg ctcgacgacg cgcgccggct gacccgcgag
      841 tcgctcgatg cgctgcgcga ccgcgcccag ctgatggacg ccgaggtcga ctcgctcgag
      901 gaccggatcg accacgccta caacccgcac tggggctcgt gcctgcgcga gggcaacgag
      961 aactcgcggt tcggcgagca ggtcaacgac tacgccgatc tctacacctc gcgggtgtcg
     1021 aacttcggcc cgtactcgcc gctgcgctac ttccgcgcgc cgcgccggcc gatgccgcac
     1081 gagatctgag cggcttttcg cgcgcgcggg gcggctgtat ggtccggctt catgccgccg
     1141 tcgccaccgc cgccccctgc acgggcgctg gcctcgatcc aggtcgcgat cgcggtggtc
     1201 ggcgtggccg tgctggcgtg gcgcgcgtcg cactactggc cgttcgtcgc ggacgacgcg
     1261 ctgatctcgc tgcgctacgc caggcgcctg gtcgccgggc acgggctgac ctggaccgac
     1321 ggcgagcggg tcgagggcta ctcgaacctg ctgtgggtgg cggcgtgcgc cgggctgcac
     1381 gcgctcggcc tcgacctcat cacggcggcg cgcgcgctcg ggctcgccgg ccacgccgcg
     1441 gtgatctgcg cgatcgtgcg gatcgcggtg gtgcgctcgc tcggcgaggc gctggcgctg
     1501 ctggcgatcg tcctggcgtt cgcgctgacc gggccgatcg acgcatgggc gatcggcggg
     1561 ctggagcagc cgctgctcgt cgcgctgctg gcgtgggcgg tcgcgctggg gctgc
//
LOCUS       DASVOR010000418         3279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_383706,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000418 DASVOR010000000
VERSION     DASVOR010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3279
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383706"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF516_08345"
     CDS             <1..237
                     /locus_tag="VF516_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107726.1"
                     /translation="APFSRAVGHLFPAAPVAITALLEAGASGMEELAAWIQAHASAPD
                     LLAEVAQLRSGASELRAVTPTDTTGGTAGPHRRP"
     gene            complement(272..862)
                     /locus_tag="VF516_08350"
     CDS             complement(272..862)
                     /locus_tag="VF516_08350"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEX8107727.1"
                     /translation="MIVCCAACSLGGKPPSYQYYVLTSRPASPAAPHVEPGAASRTLA
                     IEEVRIPGYLDREQIATRTAGQGLLYSTTDRWAEPLDKAVERTLLEDLAVLLIPHGIQ
                     VTSQGGRATYELSVDVLRFERSGADRVELWARWVLRSEGRVLDRDECRVQLPMSGGDS
                     NAVAAALSEAVARMATQVGDRVQKADVVAARGRSDG"
     gene            complement(874..1758)
                     /locus_tag="VF516_08355"
     CDS             complement(874..1758)
                     /locus_tag="VF516_08355"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8107728.1"
                     /translation="MSKHASPAKIGAFVVTALALLVTAILILGGGRLFRTTAAVIVYF
                     DDSVGGLRVGAPVKFRGIEVGTVKDVRINMAGVAVDPHHVRIPVVLEIDERQLRERGA
                     PALDLRDRKQVQRLVDQGLRAELELESFVTGVRYVELDIRPGTPARLVHDRTYPEIPP
                     VQSPTQAIPGRVDQVLAKLADLDLAGIAQSVKATVDHVDGLVSSPHLARAVGNLDALT
                     ADLDRAAAPLGPAAQDLRPAIAEAKDTVALIHRATDQLSSRLDTTLHDLSDAARSLRR
                     LADQLARDPGSILRGGKQ"
     gene            complement(1755..2519)
                     /locus_tag="VF516_08360"
     CDS             complement(1755..2519)
                     /locus_tag="VF516_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8107729.1"
                     /translation="MERSPHITVRDLTVQYGDYLVLHDVSFTVRRGDVFVIMGGSGSG
                     KSTLLNALIGLAAPATGEIMYDGESFTRAEPRERQMMLRRFGVLFQFGALWSELTLAE
                     NVALPLEELTSLGRREIRELAELKLALVGLGGFAEFYPNEISGGMRKRAGLARAMALD
                     PEILFFDEPSSGLDPINARRLDDLIIELRDSLNTTIVTVSHDLASIFATANDGVYLDA
                     HHKTVTATGDPRDLLAHPPNAHIRAFLTRTDDKEAT"
     gene            complement(2522..>3279)
                     /locus_tag="VF516_08365"
     CDS             complement(2522..>3279)
                     /locus_tag="VF516_08365"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8107730.1"
                     /translation="HRDAAHEILTAIGELTVALGRLVVGRAKFRRSDLWLQMGACGAS
                     ALGIVSLVVGLVGVIIAFISAVELKSFGASLYVANLVGIAIVRELGAVMSAIVVAGRT
                     GAAFAAELGTMRVTQEIDALTTLGLSPVEFLVVPRVLAVTLMLPLLCMYADLVGVIGG
                     AAVGIGIMHVAPRLYYDQTVHAVSLSDLIGGIVKAATYGFLIGSAGCFIGLRAGRSAA
                     AVGRAATSAVVAGLVLVIAACGLYAVAFYRLGL"
BASE COUNT          529 a         1144 c         1113 g          493 t
ORIGIN      
        1 gcaccgtttt ctcgcgccgt cggccacctc tttcccgcgg cgccggtcgc gatcacggcg
       61 ctgctcgaag ctggcgcctc cggcatggag gagctcgcgg cgtggatcca ggcccacgcg
      121 agcgctcctg atcttctcgc cgaggtcgcg cagttgcgat ccggagcctc ggagctgcgt
      181 gcggtaacgc cgactgacac aacgggagga acggccggcc ctcaccggcg cccctgagct
      241 gagtcggggt ccaggcgtcc tctcgatccg ctcagccatc ggagcgccct cgcgccgcga
      301 cgacatcggc cttctgcacc cggtcgccca cctgggtcgc catgcgcgcg acggcctcgc
      361 tgagcgcggc agccaccgcg ttgctgtcgc cgcccgacat cggaagctgc acgcgacact
      421 cgtcccggtc gaggacgcgg ccctccgagc ggaggaccca gcgcgcccac agctcgacgc
      481 gatcggcgcc gctgcgttcg aaccgcagca cgtcgacgga gagctcgtac gtcgcgcgtc
      541 caccctgcga ggtcacctgg atgccgtggg ggatcagcag gaccgcgagg tcctcgagga
      601 gcgtgcgttc gaccgccttg tcgagaggtt ctgcccagcg gtcggtggtc gaatacagga
      661 gaccctgacc ggcggtgcgg gtcgcgatct gttcgcgatc gaggtagccg gggatcctca
      721 cctcctcgat ggcgagcgtg cgcgatgcgg cacctggttc cacgtgcggt gctgcgggcg
      781 aggctggccg cgaggtgagg acgtagtact ggtagctcgg cggcttgccg ccgagcgagc
      841 aggcggcgca gcagacgatc accagcacga gcctcattgc ttgcctccgc gcaagatcga
      901 ccccggatcg cgagcgagct gatcggcgag gcggcgcagc gagcgcgcag cgtccgacag
      961 gtcatgcaac gtggtatcga gtcgactcga cagctggtcg gtcgcccggt gtatcaatgc
     1021 cacggtatcc ttcgcctccg cgatcgccgg gcgcaggtcc tgcgcggccg ggccaagcgg
     1081 ggcggcggcc cggtcgagat cggcggtgag cgcatcgagg ttgccgacgg ctcgcgcgag
     1141 atggggggag ctcaccagcc cgtcgacatg gtcgacggta gccttcaccg actgcgcgat
     1201 accggcgagg tcgaggtcgg cgagcttcgc gagcacctga tcgacccttc cggggatcgc
     1261 ctgggtcggg ctctgcaccg gtggaatctc gggatacgtg cgatcgtgga ccaggcgcgc
     1321 cggtgtgccc ggccggatgt cgagctcgac gtagcggacc ccggtgacga agctctcgag
     1381 ctcgagctcg gcgcgaagtc cctggtcgac gagccgctgc acttgcttcc ggtcgcggag
     1441 atcgagcgcg ggtgccccgc gttcgcgcag ctggcgctcg tcgatctcga gcaccaccgg
     1501 gatccgcacg tggtgcggat caacggcgac cccggccatg ttgatccgca catccttgac
     1561 cgtgccgacc tcgatgccgc ggaacttcac cggcgcgccg acgcgcagcc ccccgacgga
     1621 gtcgtcgaaa tagacgatca ccgccgcggt ggttcgaaac agtcgacctc cacccaagat
     1681 caggatcgcg gtcaccagca gcgccagcgc ggtgaccacg aacgccccga tcttggccgg
     1741 cgatgcgtgc ttgctcatgt cgcctccttg tcgtcggttc gggtcaggaa tgcccggatg
     1801 tgtgcgttcg gcggatgcgc cagcaggtcc ctcggatctc ccgttgcggt caccgtcttg
     1861 tgatgcgcgt cgaggtagac gccatcgttc gcggtcgcga agatgcttgc gagatcgtgg
     1921 ctcacggtca cgatcgtcgt gttcaggctg tcccgcagct cgatgatgag atcgtcgagg
     1981 cggcgcgcgt tgatcggatc gagccccgag gacggctcgt cgaagaacag gatctcggga
     2041 tcgagcgcca tcgcgcgcgc caggccggcc cgcttccgca tgccgccgct gatctcgttg
     2101 gggtagaact cggcaaaacc gccgagcccg acgagcgcga gcttgagctc ggccagctcg
     2161 cggatctcgc gccggccgag gctcgtgagc tcttcgagcg gcaatgcgac attctcggcc
     2221 agcgtcagct cgctccacag cgccccgaac tggaacagca cgccgaaccg acgcagcatc
     2281 atctgtcgtt cgcggggctc ggcgcgggtg aagctctcgc cgtcgtacat gatctccccg
     2341 gtcgcgggcg cggcgagccc gatcaacgcg ttcagcaggg tgctcttgcc gctgccgctg
     2401 ccgcccatga tcacgaacac gtcacctcga cgcacggtga agctcacgtc gtgcaatacc
     2461 aggtagtcgc cgtactgcac ggtgagatcc cgaaccgtga tatgagggct gcgctccatg
     2521 gctagagacc gagtcgatag aacgccaccg catacagccc gcaggccgcg atcacgagca
     2581 cgaggcctgc aaccacggcc gaggtggcgg ccctgccgac cgcggccgcg ctccgtcccg
     2641 cgcgcagccc gatgaagcag ccggccgagc cgatcaggaa gccgtaggtc gccgccttga
     2701 cgatgccgcc gatcagatcg ctgagcgaca ccgcatggac cgtctgatcg tagtacagcc
     2761 gcggcgccac gtgcatgatc ccgataccca ccgccgcgcc gccgatcacg ccgaccagat
     2821 cggcgtacat gcacagcagc ggcagcatga gggtcaccgc gaggacgcgc ggcaccacga
     2881 ggaactccac cggcgagagc cccagcgtcg tcagcgcgtc gatctcctgc gtgacccgca
     2941 tcgtgccgag ctcggcggca aaggctgcac cggtccggcc ggcgacgacg atcgcggaca
     3001 tcacggcgcc gagctcgcgc acgatggcga tcccgacgag gtttgcgacg tacagagagg
     3061 caccgaacga cttgagctcg acggccgaga tgaatgcgat gatcacccct accagtccga
     3121 cgacgagact gacgatcccg agcgcgctgg ccccgcaggc tcccatctgg agccacaggt
     3181 cgctgcgccg gaacttcgct cgcccgacaa cgagccggcc gagcgcgacc gtcagctcgc
     3241 cgatcgcggt caggatctcg tgcgctgcat cgcggtgag
//
LOCUS       DASVOR010000419         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_500805,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000419 DASVOR010000000
VERSION     DASVOR010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500805"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /gene="rplN"
                     /locus_tag="VF516_08370"
     CDS             complement(<1..252)
                     /gene="rplN"
                     /locus_tag="VF516_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005434271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEX8107731.1"
                     /translation="MIQMTSVLDVADNSGAKKVFCIKVLGGTRRRYATIGDVIIVSIR
                     EAIPGAKVKKGEVARAVIVRTKRELARPDGSYIKFDGNSA"
     gene            complement(263..616)
                     /gene="rpsQ"
                     /locus_tag="VF516_08375"
     CDS             complement(263..616)
                     /gene="rpsQ"
                     /locus_tag="VF516_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEX8107732.1"
                     /translation="MTDSTDAVSSSAPAKADQRGSRRVIVGTVTSSNMDKTATVTVIR
                     RVRDRRFHKFLTRRVKYHVHDEHNTAKVGDLVEIVESRPMSKTKRWRLLSTLQRAVEA
                     EPHAAAQADHAAEEK"
     gene            complement(613..891)
                     /gene="rpmC"
                     /locus_tag="VF516_08380"
     CDS             complement(613..891)
                     /gene="rpmC"
                     /locus_tag="VF516_08380"
                     /inference="COORDINATES: protein motif:HMM:NF013027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEX8107733.1"
                     /translation="MSKIATTLEQLRDQPDHELRQALARTRDELFRLHLAQHTNQVTS
                     TAALMGKRREIARILTILRARELGTETQGQKTQARRAAKSSKTQQAKS"
     gene            complement(888..1304)
                     /gene="rplP"
                     /locus_tag="VF516_08385"
     CDS             complement(888..1304)
                     /gene="rplP"
                     /locus_tag="VF516_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEX8107734.1"
                     /translation="MLSPKRSKFRKSHKGNLKGRAKAGNEVSFGDYGLQVTSPGWLTA
                     RQIEAARVAISRSVKKLGKIYVRVFPDHPFTKKPAETRMGTGKGGVEGWVAVVRPGRV
                     IFEVEGVSLEIAKEAFHRAHHKLTVKTQLIARESAI"
     gene            complement(1335..>1610)
                     /gene="rpsC"
                     /locus_tag="VF516_08390"
     CDS             complement(1335..>1610)
                     /gene="rpsC"
                     /locus_tag="VF516_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX8107735.1"
                     /translation="RRAMKKATQTAMKFGAKGIKVASSGRLGGAEMCRREWYKEGRVP
                     LHTLRADIEYGFTEAHTTYGSIGVKAWVFHGEVLPAARKPTSQAPQR"
BASE COUNT          202 a          530 c          578 g          300 t
ORIGIN      
        1 ggccgagttg ccgtcgaact tgatgtagct gccatcgggc cgcgccagct cgcgcttggt
       61 gcggacgatc acggcgcgcg cgacctcgcc cttcttgacc ttggcgccgg ggatcgcctc
      121 gcggatcgac acgatgatga catcgccgat cgtcgcgtag cgccggcggg tgccgccgag
      181 caccttgatg cagaacacct tcttggcgcc gctgttgtcg gcgacgtcga gcacggaggt
      241 catctggatc atggcgcgct cctcacttct cctcggctgc gtggtccgcc tgcgccgcgg
      301 cgtgcggctc ggcctcgacc gcgcgctgca gcgtgctgag cagccgccag cgcttggtct
      361 tcgacatcgg gcgcgactcg acgatctcga cgaggtcgcc gaccttggcg gtgttgtgct
      421 cgtcgtgcac gtggtacttc acgcgccggg tcaggaactt gtggaaccgg cggtcgcgga
      481 cgcggcggat caccgtgacg gtcgccgtct tgtccatgtt gctcgacgtg acggtcccga
      541 cgatgacgcg gcgcgagccg cgctgatcgg ccttcgccgg ggcggaggaa gaaacggcat
      601 cggtcgaatc ggtcatgact tcgcctgttg ggtcttggag gatttcgccg cccgccgggc
      661 ctgggtcttc tggccctggg tctcggtgcc gagctcgcgg gcgcgcagga tcgtgaggat
      721 ccgcgcgatc tcgcggcgct tgcccatcag cgcggcggtc gacgtgacct ggttggtgtg
      781 ctgggcgagg tggagccgga acagctcgtc gcgcgtgcgc gccagggcct ggcgaagctc
      841 gtggtcgggc tgatcgcgga gctgctcgag cgtggtggcg atcttgctca tatcgccgac
      901 tcccgggcga tgagctgggt cttgaccgtc agcttgtggt gggcgcggtg gaacgcctcc
      961 ttggcgatct cgagcgacac gccctcgacc tcgaagatca cccggccggg gcggaccacg
     1021 gcgacccagc cctcgacacc gcccttgccg gtacccatgc gggtctcggc cggcttcttg
     1081 gtgaacgggt gatccgggaa gacgcggacg tagatcttgc ccagcttctt gaccgagcgc
     1141 gagatcgcga cgcgggccgc ctcgatctgg cgcgcggtga gccagcccgg cgaggtgacc
     1201 tggagcccgt agtcgccgaa cgacacctcg ttgccggcct tggcgcggcc cttcaggttg
     1261 cccttgtgcg acttgcgaaa cttcgaacgt ttgggggaga gcatcgacat cgttggatct
     1321 ccgggttctc cagctcagcg ctgcggcgcc tgggacgtgg gcttcctggc agcggggagg
     1381 acctcgccgt ggaacaccca ggccttgacg ccgatgctgc cgtacgtggt gtgggcctcg
     1441 gtgaagccgt actcgatgtc cgcgcgcagg gtgtggagcg ggacgcggcc ctccttgtac
     1501 cactcgcggc ggcacatctc ggcgccgccg agccggccgc tcgacgcgac cttgatgccc
     1561 ttggcgccga acttcatcgc ggtctgggtc gccttcttca tcgcgcggcg
//
LOCUS       DASVOR010000420         6875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_149985,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000420 DASVOR010000000
VERSION     DASVOR010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149985"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..810)
                     /locus_tag="VF516_08395"
     CDS             complement(<1..810)
                     /locus_tag="VF516_08395"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8107736.1"
                     /translation="MRVARLAIAVSLFAVFLLFGLCSLPASVHAQVITATLRGTVHGA
                     DDGAPMAEVEVTLVNEATGDTRTATTNAAGAFAFTNLQVGGPYHVTANFAGFKPAEEK
                     GILLTAARTRDVDLGLRLQEEVIEVSGNAIARNTSNRTVVTAAEMDELPSVNRDPRDV
                     VRRNPEVTVEGATRALSIGGANNRYNSITIDGIRQDDDFGLNPSGYPTRRSPIGLSAV
                     QELAVDSSPFDVRYGKFLGGNVNIVTKSGTNEFKGTVFGTYASDALLGSKSG"
     gene            1264..1524
                     /locus_tag="VF516_08400"
     CDS             1264..1524
                     /locus_tag="VF516_08400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107737.1"
                     /translation="MRLLHLAAVLALAACAGPRGPHANVPKNAWVDEDPETGEELVCQ
                     DERTTGTNMSKVVCRTQDEIDEERNAAMVWETNRRLEHAKKF"
     gene            1687..1938
                     /locus_tag="VF516_08405"
     CDS             1687..1938
                     /locus_tag="VF516_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107738.1"
                     /translation="MRRIFAAGMLALIACGPSLPPPRPLGSPGADPDDDLVCHDEATT
                     GTNMVRSVCLTRAQVDENRRAAQDWEKRPHNDLGQVNKK"
     gene            1990..2964
                     /locus_tag="VF516_08410"
     CDS             1990..2964
                     /locus_tag="VF516_08410"
                     /EC_number="4.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012205355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threo-3-hydroxy-L-aspartate ammonia-lyase"
                     /protein_id="HEX8107739.1"
                     /translation="MPDLPTYTDVVAAATRLAGHAHRTPVLRSRFADALLGAEVVFKC
                     ENFQRSGAFKFRGAYSALSRFTDAQRRAGVVAFSSGNHAQAIALAAQLLGMPATIVMP
                     HDSPAIKLAATRGYGAEVVSYDRFTEDREAIGRRLAGERGMTLIPPFDHPDVIAGQGT
                     ATKELIEEVGALDTLFAPMGGAGLISGAALAARALAPHCRIYGVEPEAGNDGQQSFRS
                     GAIVHIATPRTIADGAQTQHLGELTFAIVRRELADVLTASDAQLVEAMRFFAERMKIV
                     VEPTGCLGFAAARHAGLPLRGKRVGVILSGGNIDLGRYADLLRAGADA"
     gene            3240..6512
                     /locus_tag="VF516_08415"
     CDS             3240..6512
                     /locus_tag="VF516_08415"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8107740.1"
                     /translation="MLAIVAALCGSSVAFARAGYYQFPDLHDHAIVFASEGDLWTVAD
                     TGGTARRLTTHAGAEYFPRYSPDGKTIAFTGEYGGNRDVYVIPADGGEPRRLTWHPEI
                     DEVVGWMPDGKRILFRSHAGNDTTSGFEWHLFTVPVDGGDPEPVPVGWAGRVAIDPVT
                     KRWAFNRTSVEFRTWKRYRGGTAPTIWVGDPAKGDFKEIAKAQGAEAFPMWSGGRIFF
                     LSDQGGTGNLWSMKPDGSELKRETHETTWDVRWPAVAPDGRIVYSLGGDLHVYEPATG
                     KDRKLEVDVPSERRLARVRYPSAEKSMTSFELSPDGDRLAVVTRGEIMSVAVKNGVTL
                     PITRGSAARERGAAFSGDGKSLVYITDAPGEEELRVIDAWGRGQPRVLVPAGKTGWHF
                     PPVASPDGKWIAYADQTQTLYVVPAAGGPPRTVDHSAQAEINEYVFSPDGRYLAYSLQ
                     LPTDYTSVKIFDTQTGKSASITGATTNDGTPAWDPEGRYLYFLSDRLSNPVLDRRDAE
                     NVEIRTTRPFLVLLQKDGKNPFANTAGLPPRPGEPEAKKPDDKKPDDKKPRQIKPVRI
                     DFDGLADRYLAFPVPPGNYAGLAATAKAVFYADGPVRGMAEAMDGDGPPDATLVAFDL
                     DKRKPRPFLDGVTRWKLAGDKLAIARKTDLFVVDAGKPPSPEELGEQKVAMANLVVEL
                     SPQDEWRQIYDETWRMMRDFYWDANMGGVDWRAQRDKYRALLPRLGTRDELNDLLGEL
                     IGELATSHTYIFGGDPGTELPQVATGLLGIDAVRKGIGYEVTRIYRGDPADGVRSPLD
                     EPGVNVRVGDVLLAVNHRPFAAGEPLLAALDNLADKDVVLTVNARPDFTGARDVVVHA
                     MRSERRLHYVDWVRRNREYVGQKTGGKVGYLHLPDMGANGLIAFDTWFYPQIDKQALV
                     VDVRWNGGGFVSERILERMRRKVLGAWHARSGGTGSYPARAVDGPFVVLTNEYAGSDG
                     DIFPTVVQLDKLAPVIGKRSWGGVIGIRGDKPLVDGGLPTQPEFAFWDPTQHWGTENR
                     GVIPDIEVTDLPQDVARGVDAQLDRGVAEVQRLMAQHPAAKRDFGGVPDRSRKAFEKR
                     EP"
     gene            complement(6555..>6875)
                     /locus_tag="VF516_08420"
     CDS             complement(6555..>6875)
                     /locus_tag="VF516_08420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107741.1"
                     /translation="LASALEIMGYTTRVAHDGPEALKLAAEFDPDLALLDIGLPVMDG
                     YELARRFHDNPDLRRVPLVAVTGYGQPSDRERSAAAGFDAHLVKPVDIERVIALIRQL
                     TGGR"
BASE COUNT         1052 a         2513 c         2369 g          941 t
ORIGIN      
        1 ccccgacttg ctgccgagca gcgcgtcgct cgcgtaggtg ccgaacaccg tgcccttgaa
       61 ctcgttggtg cccgacttgg tcacgatgtt gacgttgccg ccgaggaact tgccgtagcg
      121 cacgtcgaac ggcgagctgt cgaccgcgag ctcctggacc gccgacaggc cgatcgggct
      181 gcgccgggtc ggatagccgc tcgggttgag gccgaagtca tcgtcctggc ggatgccgtc
      241 gatcgtgatg ctgttgtagc ggttgttcgc gccgccgatc gacagcgcgc gggtggcgcc
      301 ctcgaccgtg acctcggggt tgcgccgcac cacgtcgcgc gggtcgcggt tgaccgacgg
      361 cagctcgtcc atctccgccg cggtcaccac ggtgcggttc gacgtgttgc gcgcgatcgc
      421 gttgcccgac acctcgatca cctcctcctg gaggcgcagc ccgaggtcga cgtcgcgggt
      481 ccgggcggcg gtgaggagga tacccttctc ctccgccggc ttgaaccccg cgaagttcgc
      541 ggtcacgtgg tacgggccgc cgacctgcag gttggtgaac gcgaacgcgc ccgcggcgtt
      601 ggtcgtcgcg gtccgggtat cgccggtcgc ttcattgacc agcgtcacct cgacctcggc
      661 catcggcgcg ccgtcgtcgg cgccgtgcac ggtcccgcgc agggtcgcgg tgatgacctg
      721 cgcatggacg ctcgcgggga gcgagcacag gccgaatagc aggaatacag cgaacagaga
      781 tacggcaatt gcgaggcggg caacacgcat gagcaacggt ccctttcgtc gaaaatgcaa
      841 tctatgcggc ctgccccctc ggacacaatc cttttggagc aagaccggcc gccgtcatca
      901 acacgtcacc tgtgccatac ggctctgacg cgctggaggc gatcccgcgc ccggacgtag
      961 tggagctcgc atcgattacg ccgcggcggc gaagcaaaaa ttcgctgccg cgacgcctac
     1021 gtaccggacg atcgacggac gcgatccgcg cgcttcgccg ttcgcaaacg cggctccggc
     1081 accaccctgg tgcgacccga cctccgggcg atcacgcaac cgctcccggt gcgacgcgcc
     1141 gccgccgggt ccgggggttt gacgccgcga gccgtgagca cggcctccgc cgagccgcac
     1201 aatgctgcac ggggccgcac gaaaacagct gcatgtttcc acgaccggga ttagcatcgg
     1261 atcatgcgct tacttcacct cgcggccgtg ctcgcgctcg ccgcgtgcgc gggccccaga
     1321 ggcccccacg cgaacgttcc caagaacgcc tgggtcgatg aagatccgga gactggcgag
     1381 gagctggtct gccaggacga gcggacgacc ggcaccaaca tgtccaaggt cgtgtgccgc
     1441 acgcaggacg agatcgacga ggagcgcaac gccgcgatgg tgtgggagac caaccggcgc
     1501 ctcgagcacg ccaagaagtt ctaggcgcgc cccgcccaag gccgccgcgg gacgacaccg
     1561 agtcaggtgc gaccaccgct gcggcgtcgg gaccgcagcg tgggcgcacg cggatcgccg
     1621 aagattgccg gtcggccacc gtcggcgtgg taccgccccc cagcgggatg cagtagcata
     1681 cgcgcgatgc gccgaatctt tgccgccggt atgctcgcac tgatcgcgtg tggaccttcc
     1741 ctgccaccgc cgcggccgct cggaagcccg ggcgccgatc ccgacgacga tctcgtgtgt
     1801 catgacgagg cgaccacggg caccaacatg gtccgctcgg tctgcctgac ccgcgcccag
     1861 gtcgacgaga accgccgcgc ggcgcaggac tgggagaagc gcccgcacaa cgatctcggc
     1921 caggtcaaca agaagtagcc gcgcgcgccc gcgccgggta ctgccaggga ctgaacgacc
     1981 gctgaccgga tgccggacct cccgacctat accgacgtcg tcgcggccgc gacgcggctc
     2041 gccggccatg cgcaccgcac gcccgtgctg cgctcgcggt tcgccgacgc gctgctcggc
     2101 gccgaggtcg tgttcaagtg cgagaacttc cagcgcagcg gcgcgttcaa gttccgcggc
     2161 gcgtacagcg cgctgtcgcg gttcaccgac gcccagcgcc gcgccggggt cgtcgcgttc
     2221 tcgtccggca accacgccca ggcgatcgcg ctggccgccc agctgctcgg catgccggcg
     2281 accatcgtga tgccgcacga tagccccgcg atcaagctcg ccgcgacccg cggctacggc
     2341 gccgaggtcg tgagctacga ccgcttcacc gaggaccgcg aggcgatcgg ccgccggctc
     2401 gccggggagc gcggcatgac cctgatcccg ccgttcgatc accccgacgt gatcgccggc
     2461 cagggcaccg cgaccaagga gctgatcgag gaggtcggcg cgctcgacac cctgttcgcg
     2521 ccgatgggcg gcgccggcct gatcagcggc gcggcgctcg ccgcccgcgc gctggcgccg
     2581 cactgccgga tctacggcgt cgagcccgag gccggcaacg acggccagca gtcgttccgc
     2641 tcgggcgcga tcgtccacat cgcgaccccc cggacgatcg ccgacggcgc gcagacccag
     2701 cacctcggcg agctgacctt cgcgatcgtc cgccgcgagc tcgccgacgt gctgaccgcc
     2761 agcgacgccc agctcgtcga ggcgatgcgg ttcttcgccg agcgcatgaa gatcgtcgtc
     2821 gagccgaccg gctgcctcgg gttcgccgcg gcgcgccacg ccggcctgcc gctgcgcggc
     2881 aagcgggtcg gcgtgatcct cagcggcggc aacatcgacc tcgggcgcta cgccgacctg
     2941 ctgcgcgccg gcgccgacgc ctgaggccgg ccgatcagga tgctcacctc cgagacctgc
     3001 ggttctgcca ttctcatgca tcgttgcgcc ggcccgacgc gcgcgtccat gacgcgcgat
     3061 tacggtggat aacggccggt tccgcagcgc gacaggttgc gacatggcgc ggtgccgcag
     3121 cgtcgaccgg cgtgctcgtg tcaaccgatc tggacttcga gacatcgatc tgatgtcgtc
     3181 tgcgctgctg ctttcccggg gggagaccca tgcacgttcg aaggtcgttt cgaagcccat
     3241 tgctggccat cgtcgctgcg ctgtgtggct cgtcggtcgc gttcgcgcgc gccggctact
     3301 atcagtttcc cgatctccac gatcacgcga tcgtgttcgc gtccgagggc gacctgtgga
     3361 ccgtggcgga caccggcggc acggcgcgcc ggctgaccac gcacgccggc gccgagtact
     3421 ttcctcgcta ctcgcccgac ggcaagacga tcgcgttcac cggcgagtac ggcggcaacc
     3481 gcgacgtcta cgtgatcccg gccgacggcg gcgagccgcg gcggctgacc tggcaccccg
     3541 agatcgacga ggtcgtcggc tggatgcccg acggcaagcg gatcctgttc cgcagccacg
     3601 ccggcaacga caccaccagc gggttcgagt ggcacctgtt cacggtgccg gtcgacggcg
     3661 gcgaccccga gccggtgccg gtcggctggg ccggccgggt cgcgatcgat ccggtgacca
     3721 agcgctgggc gttcaaccgc acgagcgtcg agttccggac ctggaagcgc taccgcggcg
     3781 gcaccgcgcc gacgatctgg gtcggcgacc ccgccaaggg cgacttcaag gagatcgcca
     3841 aggcccaggg cgccgaggcg ttcccgatgt ggtccggcgg ccggatcttc ttcctgtccg
     3901 atcagggcgg caccggcaac ctgtggtcga tgaagcccga cggcagcgag ctcaagcgcg
     3961 agacccacga gaccacctgg gacgtgcgct ggccggcggt cgctcccgac ggccggatcg
     4021 tgtactcgct cggcggcgat ctccacgtct acgagcccgc caccggcaag gaccgcaagc
     4081 tggaggtcga cgtcccgagc gagcgccggc tcgcccgcgt ccgctacccc agcgccgaga
     4141 agtcgatgac gtcgttcgag ctgtcgcccg acggcgatcg gctcgcggtg gtgacccgcg
     4201 gcgagatcat gtcggtcgcg gtcaagaacg gcgtgaccct gccgatcacc cggggcagcg
     4261 cggcgcgcga gcgcggcgcg gcgttctcgg gcgacggcaa gagcctggtc tacatcaccg
     4321 acgcgcccgg cgaggaggag ctgcgcgtga tcgacgcgtg gggccgcggt cagccgcgcg
     4381 tgctggtgcc cgcgggcaag accggctggc acttcccgcc ggtggcctcg cccgacggca
     4441 agtggatcgc gtacgccgat cagacccaga ccctgtacgt cgtccccgcc gcgggcgggc
     4501 cgccccgcac cgtcgatcac agcgcgcagg ccgagatcaa cgagtacgtg ttcagccccg
     4561 acggccgcta cctcgcctac tcgctgcagc tgcccaccga ctacacctcg gtgaagatct
     4621 tcgacaccca gaccgggaag tcggcgtcga tcaccggcgc cacgaccaac gacggcacgc
     4681 cggcgtggga ccccgagggc cgctacctgt acttcctgag cgaccggctc tccaaccccg
     4741 tgctcgaccg ccgcgacgcg gagaacgtcg agatccggac cacgcggccg ttcctggtcc
     4801 tgctgcagaa ggacggcaag aacccgttcg ccaacaccgc cggcctgccg ccgcggccgg
     4861 gcgagcccga ggccaagaag cccgacgaca agaagcccga cgacaagaag cccaggcaga
     4921 tcaagccggt ccggatcgac ttcgacggcc tcgccgaccg ctacctcgcg ttccccgtgc
     4981 cgcccggcaa ctacgccggg ctcgcggcga ccgccaaggc ggtgttctac gccgacggcc
     5041 cggtgcgcgg catggccgag gccatggacg gcgacggccc gcccgacgcc acgctggtcg
     5101 cgttcgatct cgacaagcgc aagcccaggc cgttcctcga cggcgtgacc cggtggaagc
     5161 tcgccggcga caagctcgcg atcgccagga agaccgacct gttcgtggtc gacgccggca
     5221 agccgccgtc ccccgaggag ctcggcgagc agaaggtggc gatggccaac ctggtcgtcg
     5281 agctgtcgcc gcaggacgag tggcggcaga tctacgacga gacgtggcgg atgatgcgcg
     5341 acttctactg ggacgccaac atgggcggcg tcgactggcg cgcccagcgc gacaagtacc
     5401 gcgcgctgct gccccggctc ggcacgcgcg acgagctcaa cgacctgctc ggcgagctga
     5461 tcggcgagct cgcgacctcg cacacctaca tcttcggcgg cgatccgggc accgagctgc
     5521 cgcaggtggc gaccggcctg ctcgggatcg acgccgtgcg caagggcatc ggctacgagg
     5581 tgacgcggat ctatcgcggc gatccggccg acggcgtgcg atcgccgctc gacgagcccg
     5641 gggtcaacgt ccgggtcggc gacgtcctgc tcgcggtcaa ccaccggcca ttcgccgccg
     5701 gcgagccgct gctcgccgcg ctcgacaacc tcgccgacaa ggacgtcgtg ctcaccgtca
     5761 acgcgcgccc ggatttcacc ggcgcccgcg acgtggtggt ccacgcgatg cgctcggagc
     5821 gccggctgca ctacgtcgac tgggtccgcc gcaaccgcga gtacgtcggc cagaagaccg
     5881 gcggcaaggt cggctacctc cacctgccgg acatgggcgc caacggcctg atcgcgttcg
     5941 acacctggtt ctatccccag atcgacaagc aggcgctggt cgtcgatgtc cggtggaacg
     6001 gcggcggctt cgtgtcggag cgcatcctcg agcgcatgcg ccgcaaggtg ctcggcgcgt
     6061 ggcacgcccg cagcggcggc accgggagct acccggcgcg cgcggtcgac ggcccgttcg
     6121 tcgtgctgac caacgagtac gccggctccg acggtgacat cttcccgacc gtggtccagc
     6181 tcgacaagct cgccccggtg atcggcaagc gctcgtgggg cggcgtcatc gggatccgcg
     6241 gcgacaagcc gctggtcgac ggcggcctgc cgacccagcc ggagttcgcg ttctgggatc
     6301 cgacccagca ctgggggacc gagaaccgcg gcgtgatccc cgacatcgag gtcaccgacc
     6361 tgccgcagga cgtcgcccgc ggggtcgatg cccagctcga ccgcggcgtg gccgaggtcc
     6421 agcgcctgat ggcgcagcac ccggcggcca agcgcgactt cggcggcgtc cccgaccgca
     6481 gccgcaaggc gttcgagaag cgcgagccgt gacccgcagc gggcgccgcc acgcgcgcgc
     6541 ggtgccccac ccgctcatcg ccccccggtg agctgccgga tcagcgcgat gacccgctcg
     6601 atgtcgaccg gcttgacgag gtgcgcgtcg aagccggcgg ccgcggatcg ctcgcggtcg
     6661 gacggctgtc cgtagccggt caccgcgacg aggggcacgc gccgcaggtc cgggttgtcg
     6721 tggaaccggc gcgccagctc gtagccatcc atcaccggca ggccgatgtc gagcagcgcg
     6781 agatcgggat cgaactcggc cgccagcttc agcgcctcgg ggccatcgtg cgcgacgcgg
     6841 gtcgtgtagc ccatgatctc cagcgcgctc gcgag
//
LOCUS       DASVOR010000421         1945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_91848,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000421 DASVOR010000000
VERSION     DASVOR010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91848"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..>1945
                     /locus_tag="VF516_08425"
     CDS             120..>1945
                     /locus_tag="VF516_08425"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HEX8107742.1"
                     /translation="MRFAVAHKVASYLMVGCAYCALVAGGGVSPMIAIGGVIGLVASW
                     WWEPPLIRIERWSLWWTIGSLVALVYSVLAAVASGDFLELGAQFLIWLIVAKAFNRRA
                     ARDWQQMYLLAFLMLVAGSVLNPDLTYGLCFLGFVIASTWALTLFHLRREMEDNLLVK
                     HAADRASERVEVRRILDSRRIVNGRFFLGTGALSFAVFLFAALVFLALPRVGVGFFLK
                     ARGGLTLAGFADGVKLGGHGVIKNDATVVMRVEIAPRYGGRDAPAIHWRGVAFDHYEH
                     GQWSRQLGSPRTLQTLEQSATRDRRYLRWDGPPLTSAAIDALAGQLVKQDIWLDPLDS
                     IDVLFGASAPRIVEYTHTLRPRRPLYEHNDEIRLEHGTTVHYTVWSQLTSPPADALRA
                     AAGKLPESYQPYLQLPREITPRTRELARTITAGLTTEYDKVQAIRAWLVGNLTYTLVL
                     AEPGAQEPVDFFLFDRKKGHCEYFASAFAVLARAVAIPTRQVNGFLGGEWNEYQGYVA
                     VRAGDAHSWDEVYYPGVGWVTVDPTPPANVDALGRGGSGWTARLGRFLDTLRFQWNKW
                     VIEYDLTSQLALFNEVGGALRSAGEALKHAAAGVRDGVAHHGP"
BASE COUNT          245 a          697 c          704 g          299 t
ORIGIN      
        1 ccctacgcgt cgcccgagct ggcgttcgcg gtgctgccgc cccgggtcga cagcgtgctc
       61 gtcgtgccgc gcgcggtggc ggcggcgggc cggcccaggg ccgccacggt gatggacgta
      121 tgaggttcgc cgtcgcccac aaggtcgcga gctacctgat ggtgggctgc gcgtactgcg
      181 cgctggtcgc cggcggcggg gtctcgccga tgatcgcgat cggcggcgtg atcggcctcg
      241 tcgcgtcgtg gtggtgggag ccgccgctga tccggatcga gcgctggtcg ctgtggtgga
      301 cgatcggctc gctggtcgcg ctggtctaca gcgtgctggc cgcggtggcc agcggcgact
      361 tcctcgagct cggcgcgcag ttcctgatct ggctgatcgt cgccaaggcg ttcaaccgcc
      421 gggccgcgcg cgactggcag cagatgtacc tgctcgcgtt cctcatgctg gtcgccggct
      481 cggtgctcaa ccccgatctg acctacgggc tgtgcttcct cggcttcgtg atcgcgtcga
      541 cctgggcgct cacgctgttc catttgcggc gcgagatgga ggacaacctc ctggtcaagc
      601 acgccgcgga tcgcgcgtcg gagcgggtcg aggtccgccg catcctcgac tcgcggcgga
      661 tcgtcaacgg ccggttcttc ctcggcaccg gcgcgctgtc gttcgcggtg ttcctgttcg
      721 ccgcgctggt gttcctcgcg ctgccccggg tcggcgtcgg gttcttcctc aaggcgcgcg
      781 gcggcctgac gctcgccggg ttcgccgacg gcgtcaagct cggcggccac ggcgtgatca
      841 agaacgacgc cacggtggtg atgcgggtcg agatcgcccc ccgctacggc ggccgcgacg
      901 cgccggcgat ccactggcgc ggagtcgcgt tcgaccacta cgagcacggc cagtggtcgc
      961 gccagctggg ctcgccgcgc acgctgcaga cgctcgagca gagcgcgacc cgcgaccggc
     1021 gctacctgcg atgggacggg ccgccgctga cctcggcggc gatcgacgcg ctcgccggcc
     1081 agctggtcaa gcaggacatc tggctcgatc cgctggactc catcgacgtg ctattcgggg
     1141 cgagcgcgcc gcggatcgtc gagtacaccc acaccctgcg gccgcgccgg ccgctgtacg
     1201 agcacaacga cgagatccgg ctcgagcacg gcaccaccgt gcactacacg gtgtggtcgc
     1261 agctgacctc gccgcccgcc gacgcgctgc gcgccgccgc cggcaagctg cccgagagct
     1321 accagcccta cctccagctg ccgcgcgaga tcacgccgcg gacccgcgag ctcgcgcgca
     1381 cgatcaccgc gggtctcacc accgagtacg acaaggtcca ggcgatccgg gcgtggctgg
     1441 tcggcaacct gacctacacg ctggtgctcg ccgagccggg cgcgcaggag ccggtcgact
     1501 tcttcctgtt cgatcgcaag aagggccact gcgagtactt cgcgtcggcg ttcgcggtgc
     1561 tcgcgcgcgc ggtcgcgatc ccgacccgcc aggtcaacgg cttcctcggc ggcgagtgga
     1621 acgagtacca gggctacgtc gcggtccgcg ccggcgacgc gcactcgtgg gacgaggtgt
     1681 actacccggg agtcggatgg gtcacggtcg accccacgcc gccggccaac gtggacgcgc
     1741 tcggtcgcgg cggcagcggc tggaccgcgc ggctcggccg gttcctcgac accctgcggt
     1801 tccagtggaa caagtgggtg atcgagtacg acctgacgtc gcagctggcg ctgttcaacg
     1861 aggtcggcgg cgcgctgcgc agcgcgggcg aggcgctcaa gcacgcggcc gccggcgtcc
     1921 gggacggcgt ggcgcaccac ggccc
//
LOCUS       DASVOR010000422         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208511,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000422 DASVOR010000000
VERSION     DASVOR010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208511"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            287..895
                     /locus_tag="VF516_08430"
     CDS             287..895
                     /locus_tag="VF516_08430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8107743.1"
                     /translation="MAPDASPAIRIECATGAEIARYLDALAALRIGVFREYPYLYEGS
                     LAYEHEYLASYAGSPASVVVLACDGDRVVGASTALPLTLHSDDVVPPLARAGFDPASV
                     YYFGESVLEPAYRGRGLGSRFFEERERRARELGFAIATFCAVERPADHPLRPPGYQPP
                     GALWRRHGFERRSDIVGTFSWRDVGETEETAKPMVFWIKPLA"
     gene            933..1331
                     /locus_tag="VF516_08435"
     CDS             933..1331
                     /locus_tag="VF516_08435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketosteroid isomerase-related protein"
                     /protein_id="HEX8107744.1"
                     /translation="MTDTELVTRYYDLFNRGDWDGMCALLREDVSHDLNQGERQVGRA
                     AFRAFLDRMARSYREQLRDVCVMGNGAGRIGAEYVVEGAYLATDEGLPEARGQRYTLP
                     GGAFFEIAGGQIARVTNYYNLQDWLRQVRQ"
     gene            1328..>2203
                     /locus_tag="VF516_08440"
     CDS             1328..>2203
                     /locus_tag="VF516_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8107745.1"
                     /translation="MTTLRVAAWQYPIEHLASWDAWLAKLDAGFAEARGRGAALCVVP
                     EYAAMELTSLTGGDDLAAQLDALQPVLPRYLDAYAALARRLDLAIVAGSFPERLASGE
                     VRNRLRVHTPGGVAVIEKLQMTRFERERWGVAPGRAQAMIDLGAYRLGVAICYDAEFP
                     LVVRRLVAAGADVIAVPSCTDTPAGYHRVRVACQARALENQCYVVQAPTVGVAPWSIA
                     VDENVGAAGVYGPPDRGFPDDGVIALGAYCEPGWVIADLDLDAIARVRADGQVLGHRD
                     WDLPGHLAAPVGRAQL"
BASE COUNT          270 a          799 c          813 g          321 t
ORIGIN      
        1 ggcggcgatg actcgaccgt tcgcgacccc gagcccccgc acccgacgat cgcgacgagc
       61 cacagacatc tcatcctctg atcatgccga agcgtccggc aaccagcaat acgcgacgcg
      121 ccgtccccgc cgggcccaca cggtcctcgg cgcgccgcgg ctgcagcgcc gggcgtcaac
      181 ccgaggagtt cgccacgttt ccggcaccgt tgggtgcgcc tacggccgcc gcttcttgcc
      241 gggcgttccg cgttgcgtcg tggttcaccg gcataccatg cgccgcatgg ctcccgatgc
      301 ctcccccgcg atccggatcg agtgcgcaac cggcgcagag atcgcacgtt acctcgacgc
      361 gctcgccgcg ctgcggatcg gcgtgttccg cgagtacccg tacctgtacg agggctcgct
      421 cgcgtacgag cacgagtacc tcgcgagcta cgccggctcg ccggcgagcg tggtcgtcct
      481 cgcgtgcgac ggcgaccgcg tggtcggcgc gtcgaccgcg ctgccgctga cgctgcactc
      541 cgacgacgtc gtgccgccgc tcgcccgcgc cggcttcgat cccgcgagcg tgtactactt
      601 cggcgagtcg gtgctggagc cggcgtaccg cggccgtggg ctgggcagcc ggttcttcga
      661 ggagcgcgag cggcgggccc gcgagctggg gttcgcgatc gcgacgttct gcgccgtcga
      721 gcggccggcg gatcacccgc tccggccgcc gggctaccag ccgcccggcg cgctgtggcg
      781 gcgccacggg ttcgagcgcc ggtccgacat cgtcggcacg ttctcgtggc gcgacgtcgg
      841 cgagaccgag gagaccgcca agccgatggt gttctggatc aaaccgctcg cgtgatgtgc
      901 atcgcgaccg tgcggcgggc tatggttgcg gcgtgaccga taccgagctg gtgacgcgct
      961 actacgatct gttcaaccgc ggtgactggg atggcatgtg cgcgctgctg cgcgaggacg
     1021 tgagccacga cctgaaccag ggcgagcggc aggtcggccg ggcggcgttc cgcgcgttcc
     1081 tcgatcgcat ggcgcggtcg tatcgcgagc agctgcgcga cgtgtgcgtg atgggcaacg
     1141 gcgcagggcg gatcggggcg gagtacgtcg tcgagggcgc gtacctcgcc accgacgagg
     1201 gcctgcccga ggcgcgcggc cagcgctaca cgctgccggg cggcgcgttc ttcgagatcg
     1261 cgggcgggca gatcgcgcgg gtcaccaact actacaacct gcaggactgg ctgcgtcagg
     1321 tccggcagtg accacgctgc gcgtcgccgc ctggcagtac ccgatcgagc acctggcgtc
     1381 gtgggacgcg tggctggcca agctcgacgc cggcttcgcc gaggcgcgcg gccgcggcgc
     1441 agcgctgtgc gtggtccccg agtacgcggc gatggagctg acgtcgttga ccggcggcga
     1501 cgatctggcc gcgcagctcg acgcgctgca gccggtcctg ccgcgctacc tcgacgccta
     1561 cgccgcgctc gcgcgccgcc tggacctcgc gatcgtcgcc ggcagcttcc cggagcgcct
     1621 cgcgtcgggc gaggtccgca accggctgcg cgtccacacg cccggcggcg tggccgtcat
     1681 cgagaagctg cagatgacgc gcttcgagcg cgagcgctgg ggcgtcgcgc cgggccgcgc
     1741 gcaggccatg atcgatctcg gcgcgtaccg gctcggcgtc gcgatctgct acgacgccga
     1801 gttcccgctc gtggtgcgcc ggctggtcgc ggcgggcgcg gacgtcatcg cggtgccgag
     1861 ctgcaccgac acgccggccg gctatcaccg ggtccgcgtc gcctgccagg cgcgcgcgct
     1921 cgagaaccag tgctacgtgg tccaggcgcc gacggtcgga gtcgcgccgt ggtcgatcgc
     1981 ggtcgacgag aacgtcgggg ccgccggggt gtacgggccg cccgatcgcg gcttccccga
     2041 cgatggcgtg atcgcgctgg gcgcgtactg cgagcctggc tgggtgatcg ccgacctcga
     2101 tctcgacgcg atcgcccggg tccgcgccga cggccaggtg ctcggtcacc gcgactggga
     2161 tctgccgggc cacctcgccg cgccggtcgg gcgcgcgcag ctc
//
LOCUS       DASVOR010000423         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_442235,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000423 DASVOR010000000
VERSION     DASVOR010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442235"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..1115)
                     /locus_tag="VF516_08445"
     CDS             complement(21..1115)
                     /locus_tag="VF516_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8107746.1"
                     /translation="MTRSVWTHTTFAVICGAAVSAALGAVLGLHGALAAPAADKAAAK
                     PAADRALTPYVEEHTHFEAADAEGAVRAALAGLARQNAAMVLLQMPPDTFDHPGHYDA
                     EVVLAEAKKHPGKIGVLGGGGTLNAMIIQAAATGDAGPAVQKKFKDRAEELLRKGVIG
                     FGEMTSEHYAGVTPYQYAPSDHPLYLLLADIAAEHGVPIVLHMEAVPEDMAVPASVKV
                     PPNPPRLHANIAAFERLLAHNPRAQIIWAHAGADGTGFRTPEVCRRLLKAHPNLTMEI
                     KADPKAQGLNYPRADGKIKPDWLALFTEFPDRFVIGSDQHYPETADPVQRWQEVVRLF
                     NQLPEPVRRKIGTENVARIYGKAVAPSLKH"
     gene            complement(1178..2470)
                     /locus_tag="VF516_08450"
     CDS             complement(1178..2470)
                     /locus_tag="VF516_08450"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8107747.1"
                     /translation="MRATVLALVCSAAVALSTTATAKTVVVTADKLVDVATGKQTDRP
                     QVVIVDGRITQVGKQGDAVPAGATRVDLPGVTLLPGLIDMHTHLTTSPLFAGYNRLLF
                     SDAFWAVISTSHARATLEAGFTTVRNVGSRDYDDVGLRQAIDGGFVEGPRVWTATYAI
                     GSTGGHCDSTFFPPSMAQKSPAVIDSPLAGRQMVRQLHKYGAQVIKICATGGVFSHGD
                     TPGAQQLTLDEIKAIVDEAHMAGLKVAAHAHGASGIKAAILGGVDTIEHASLVDDEGI
                     RIARDKGAYFGMDIYNTDYTQAEGKKNGVLEENLQKDRDIALIQRQNFQKALRAGVKM
                     IFSTDAGVYPHGTNARQLATMVKWGATPLQAIQSATITAAAALGTPGDVGQVKVGAYG
                     DLVGVSGDPLKDVTLLERPVFVMKGGEVVRRAAAAPGK"
BASE COUNT          328 a          900 c          970 g          452 t
ORIGIN      
        1 agtgcttcgt cagtgcttcg tcagtgcttc agggacggcg ccacggcctt gccgtagatc
       61 cgcgcgacgt tctcggtgcc gatcttcctg cgcacgggct cggggagctg gttgaacagc
      121 cgcaccacct cctgccagcg ctgcaccgga tcggcggtct ccgggtagtg ctggtcgctg
      181 ccgatcacga agcggtccgg gaactcggtg aacagcgcga gccagtcggg cttgatcttg
      241 ccgtcggccc tcgggtagtt gaggccctgc gccttgggat cggccttgat ctccatggtg
      301 aggttcgggt gcgccttcag caggcggcgg cacacctcgg gcgtgcggaa cccggtgccg
      361 tcggcgccgg cgtgggccca gatgatctgg gcgcgcgggt tgtgcgccag cagccgctcg
      421 aacgcggcga tgttggcgtg cagcctcggc gggttgggcg gcaccttcac gctggccggc
      481 accgccatgt cctcgggcac ggcctccatg tgcaggacga tcggcacgcc gtgctcggcg
      541 gcgatgtcgg cgagcaggag gtacagcggg tgatcggacg gcgcgtactg gtacggcgtc
      601 acgccggcgt aatgctcgga ggtcatctcg ccgaacccga tcacgccctt gcgcagcagc
      661 tcctcggcgc ggtccttgaa cttcttctgc accgcggggc cggcgtcccc ggtcgccgcg
      721 gcctggatga tcatcgcgtt gagcgtgccg ccgccaccga gcacgccgat cttgccgggg
      781 tgcttcttgg cctcggcgag cacgacctcg gcgtcgtagt ggccggggtg atcgaaggtg
      841 tcgggcggca tctggagcag gaccatcgcg gcgttctggc gggccaggcc ggcgagcgcg
      901 gcgcgcaccg cgccctcggc gtcggcggcc tcgaagtggg tgtgctcctc gacgtacggg
      961 gtcagcgccc ggtcggccgc gggcttcgcg gcggccttgt cggcggctgg cgcggcgagg
     1021 gcgccgtgca ggccgagcac cgcgccgagg gctgcggaga ccgccgcgcc acagatcacc
     1081 gcgaacgtgg tgtgggtcca gacggatcgg gtcatggttg cctcgctggt tgctgcctcg
     1141 ctggttgcct tgctcgctgg ttgccttgct cgctgcttca cttgcccggc gccgccgcgg
     1201 cgcgccggac gacctcgccg cccttcatca cgaacacagg gcgctcgagc agcgtgacgt
     1261 ccttgagcgg atcgcccgac acgccgacga gatcgccgta ggcgccgacc ttgacctgac
     1321 cgacgtcgcc gggagtgccg agcgcggccg cggcggtgat cgtcgccgac tggatcgcct
     1381 gcagcggggt cgcgccccac ttcaccatgg tcgcgagctg cctggcgttg gtgccgtgcg
     1441 gatagacacc cgcatcggtc gagaagatca tcttcacgcc ggcccggagc gccttctgga
     1501 agttctggcg ctggatcagc gcgatgtcgc ggtccttctg gaggttctcc tcgagcacac
     1561 cgttcttctt gccctcggcc tgggtgtagt cggtgttgta gatgtccatg ccgaagtagg
     1621 cgcccttgtc gcgcgcgatc cggatgccct cgtcgtcgac caggctcgcg tgctcgatcg
     1681 tgtcgacccc gccgaggatc gccgccttga tacccgaggc gccgtgggcg tgcgcggcga
     1741 ccttgaggcc ggccatgtgg gcctcgtcga cgatcgcctt gatctcgtcg agcgtcagct
     1801 gctgcgcccc cggcgtgtcg ccgtgcgaga acacgccgcc ggtcgcgcag atcttgatga
     1861 cctgcgcgcc gtacttgtgg agctggcgca ccatctggcg gccggcgagc gggctgtcga
     1921 tcacggcggg gctcttctgc gccatcgacg gcgggaagaa cgtcgagtcg cagtggccgc
     1981 cggtcgaccc gatcgcgtag gtcgcggtcc acacccgcgg gccctcgacg aagccgccgt
     2041 cgatcgcctg gcgcaggccg acgtcgtcgt agtcgcgcga gccgacgttg cgcaccgtgg
     2101 tgaacccggc ctccagggtc gccctggcgt ggctggtcga gatcacggcc cagaaggcat
     2161 cggagaacag caagcggttg tagccggcaa acagcggcga tgtggtgagg tgggtgtgca
     2221 tgtcgatcag gccgggcagc agggtcacgc ccggcaggtc gacgcgggtc gcgccggcgg
     2281 ggaccgcgtc gccctgcttg ccaacctgtg tgatccgtcc gtccacgatc accacctgcg
     2341 gccggtcggt ctgcttgccg gtggcgacgt cgaccagctt gtcggcggtg acgaccaccg
     2401 tcttggcggt ggcggtggta ctcagtgcga cagcggcgct gcacacgagg gcgaggacgg
     2461 ttgcgcgcat gagctctcca gggaagcgcg gcgaggctat catgtcgaga ccatgcacac
     2521 ctcgaatccg cgcttccgga tcctgtccgc gcccctggcc ctggtggtcg cctccgcggg
     2581 ctgcggcggc tccgggaact cccagccgcc gctgccgctc ggcgaggtca acgcggcggc
     2641 ggtcgcggcc
//
LOCUS       DASVOR010000424         4543 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_559387,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000424 DASVOR010000000
VERSION     DASVOR010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4543
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559387"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3032)
                     /locus_tag="VF516_08455"
     CDS             complement(<1..3032)
                     /locus_tag="VF516_08455"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8107748.1"
                     /translation="MQRFRFVLACALAVMPLAASISAPFAVPSARAQPIGDWGVKRDP
                     FNQTDIARYKAILRTNPHDASALAKLLEMYRRYRTIDLLKDEYGKLLDKNADDVPALI
                     VMGRLQHTTGDDARALELFQRAVARKDDDAQAWLLIGELEKAASRPKDARTAYDKALA
                     HAPQKDMKKKALRALADLALTTGDNDGANAYFQQFLELDPRNAQLWIERGDAMLAAGK
                     RDVALDSYTAAEKLLGADPAKRVEVVARRGQTLEGMGKDDEAVVEYRRAIKLAPKGYY
                     LEVELTGRIIDIYRRKQSLPALLAQYEKEWPEAARGHFEWDTLGKLYEETGAQDKAIA
                     ALKRAVAGAPWELETRRRQIQLLENSGRDDEALVAYEAVVRAAPGEARFQLDLAERYW
                     RRGQDKKALDTLVRLEVRFPQDAGVLSAIADMYTRWGKEDLAIAEYERLAKLEPDDPG
                     HLVTLGEQYWAKGDKPRAIATWKRLTLSGKPGGYAKLGEVMAEHNQPGEARVSFDKAV
                     SLDPKSPELYKARAAFLETQKQYPEALADWEKVLELVGTKVTDRLARRDARRHYVTVV
                     TKIGGAREIRQKQDWDDRARAGDLEAGYLLVDYYARRPQHDQPLLALQDLHKKVPDDQ
                     DLTLDLVKAYRDQRKFDQAVATLKELLKIAPSREREIYSMISGIMTEERKDDEAEEWM
                     KKALAKSPNDPTAYEHLAENYVAMQRFADAIAAYEKTVQLDPHNSKAEFALAQLYVQG
                     GQPMKAAELLRKVLRTSSDEETIGHAGRQAIDLEEMTNTLGELEKVLSPLSFMMAHKP
                     IYRRVLVELYLRYVPRLVERAKHGSDDVRQAARAELARIGGHGLQPLLEALRDEKETS
                     QQRVAVAVLGHLGNKGAAAPLVHMARVEPSKDVRRLGTLQENLDREVRVDALVAAGRL
                     GDPAVLGDVLPLMDQKEIAMREAATFALGRSADKRAVAPLIKALDDSRQSVQVLACLG
                     LAQIDDPRVGPALIKTLSDARRHDAVRGACGYALGARRL"
     gene            complement(3147..3509)
                     /locus_tag="VF516_08460"
     CDS             complement(3147..3509)
                     /locus_tag="VF516_08460"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8107749.1"
                     /translation="MQDRRLGFRIPFETMVTSYVHDRPVRGLATNLSDSGLSVSAISM
                     LAPPPGMIVALELEVPGVDDSIWAAAQVRYRQDDHLASGLGMRFVAMAKSHARLIREF
                     CIEMRRKNLGALLARIRA"
     gene            3724..4116
                     /locus_tag="VF516_08465"
     CDS             3724..4116
                     /locus_tag="VF516_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX8107750.1"
                     /translation="MPVYEYECKACGRDFEYQQRMSDPDKTTCEVCGGALDRLISRTA
                     FSLKGGGWYKDLYASPKPEKADKSDKAAGTESAKSDGAAATTAKSDGGSTAGSSSTST
                     ASSSSTSTASSGSSSGSGSSSAAAAKAT"
BASE COUNT          622 a         1578 c         1609 g          734 t
ORIGIN      
        1 agccggcggg cgccgagcgc gtagccgcac gcgccgcgca ccgcgtcgtg ccggcgcgcg
       61 tccgacaggg tcttgatcag cgccgggccg acccgcggat cgtcgatctg ggccagcccg
      121 aggcacgcca ggacctggac cgactgccgg ctgtcgtcga gcgccttgat cagcggcgcg
      181 accgcgcgct tgtcggccga gcggccgagc gcgaacgtcg ccgcctcgcg catcgcgatc
      241 tccttctggt ccatcagcgg cagcacgtcg cccagcacgg cggggtcgcc gagccggccg
      301 gcggcgacca gcgcgtcgac ccggacctcg cgatcgaggt tctcctgcag cgtgccgagc
      361 cggcggacgt ccttgctcgg ctcgacccgc gccatgtgga ccagcggggc ggccgcgccc
      421 ttgttgccga ggtggccgag cacggcgacc gcgacccgct gctggctggt ctccttctcg
      481 tcgcgcaggg cttccagcag gggctgcagg ccgtggccgc cgatccgggc cagctcggcg
      541 cgcgccgcct ggcggacgtc gtcgctgccg tgcttggcgc gctcgaccag ccgcggcacg
      601 tagcgcaggt agagctcgac cagcacgcgg cggtagatcg gcttgtgcgc catcatgaac
      661 gacagcggcg acagcacctt ctcgagctcg ccgagcgtgt tggtcatctc ctcgaggtcg
      721 atcgcctggc ggccggcgtg gccgatggtc tcctcgtcgc tggacgtccg cagcaccttg
      781 cgcagcagct cggcggcctt catcggctgg ccgccctgga cgtagagctg ggcgagcgcg
      841 aactcggcct tgctgttgtg cgggtcgagc tgcacggtct tctcgtaggc cgcgatcgcg
      901 tcggcgaacc gctgcatcgc gacgtagttc tcggccaggt gctcgtacgc cgtcgggtcg
      961 ttggggctct tggcgagcgc cttcttcatc cactcctcgg cctcgtcgtc cttgcgctcc
     1021 tcggtcatga tccccgagat catcgagtag atctcgcgct cgcgcgacgg cgcgatcttg
     1081 agcagctcct tgagcgtggc gaccgcctgg tcgaacttgc gctggtcgcg gtaggccttg
     1141 accaggtcga gcgtgaggtc ctgatcgtcg ggcaccttct tgtggaggtc ctgcagcgcg
     1201 agcagcggct ggtcgtgctg cggccgccgc gcgtagtagt cgaccagcag gtagccggcc
     1261 tcgaggtcgc ccgcgcgcgc ccgatcgtcc cagtcctgct tctggcggat ctcgcgggcg
     1321 ccgccgatct tggtgaccac ggtgacgtag tgccggcggg cgtcgcgccg cgcgagccgg
     1381 tcggtgacct tggtgccgac cagctcgagc accttctccc agtcggcgag cgcctcgggg
     1441 tactgcttct gggtctcgag gaacgcggcg cgcgccttgt agagctcggg gctcttcggg
     1501 tcgaggctga ccgccttgtc gaagctgacc cgggcctcgc cgggctggtt gtgctcggcc
     1561 atcacctcgc cgagcttggc gtagccgccc ggcttgccgc tcagggtcag ccgcttccag
     1621 gtcgcgatcg cacgcggctt gtcgcccttg gcccagtact gctcgccgag cgtgaccagg
     1681 tggccggggt cgtcgggctc gagcttggcg agccgctcgt actcggcgat cgcgaggtcc
     1741 tccttgcccc accgggtgta catgtcggcg atcgccgaca gcacgccggc gtcctgcggg
     1801 aaccggacct cgagccggac cagcgtgtcg agcgccttct tgtcctggcc gcgccgccag
     1861 tagcgctcgg cgaggtcgag ctggaaccgg gcctcgccgg gggccgcgcg gaccaccgcc
     1921 tcgtaggcga ccagcgcctc gtcgtcgcgc cccgagttct ccaggagctg gatctgccgg
     1981 cgccgggtct cgagctccca cggcgcgccg gcgaccgcgc gcttgagcgc cgcgatcgcc
     2041 ttgtcctggg cgccggtctc ctcgtagagc ttgccgagcg tgtcccactc gaagtggccg
     2101 cgcgcggcct cgggccactc cttctcgtac tgcgcgagca gcgcgggcag cgactgcttg
     2161 cggcggtaga tgtcgatgat ccggccggtg agctcgacct cgaggtagta gcccttgggc
     2221 gcgagcttga tcgcgcgccg gtactcgacg accgcctcgt cgtccttgcc catgccctcg
     2281 agggtctggc cgcggcgcgc gacgacctcg acccgcttgg ccggatcggc gccgagcagc
     2341 ttctcggccg cggtgtagct gtcgagcgcg acgtcgcgct tgccggcggc gagcatcgcg
     2401 tcgccgcgct cgatccacag ctgggcgttc ctgggatcga gctcgaggaa ctgctggaag
     2461 taggcgttgg cgccgtcgtt gtcgccggtg gtcagcgcga ggtcggcgag cgcgcgcagc
     2521 gccttcttct tcatgtcctt ctgcggcgcg tgggccagcg ccttgtcgta ggccgtgcgc
     2581 gcgtccttgg gccggctcgc cgccttctcg agctcgccga tcaggagcca ggcctgggcg
     2641 tcgtcgtcct tcctggcgac cgcgcgctgg aacagctcga gcgcgcgggc gtcgtcgccg
     2701 gtggtgtgct gcagccggcc catcacgatc agcgccggga cgtcgtcggc gttcttgtcg
     2761 agcagcttgc cgtactcgtc cttgagcagg tcgatcgtcc ggtaccgccg gtacatctcg
     2821 agcagcttgg cgagcgccga ggcgtcgtgc gggttggtcc gcaggatcgc cttgtagcgg
     2881 gcgatgtcgg tctggttgaa cgggtcgcgc ttgacccccc agtcgccgat cggctgcgcg
     2941 cgcgcggaag ggacggcgaa cggagcggag atcgaagcgg cgaggggcat gaccgcgagg
     3001 gcacacgcca ggacaaatcg gaaccgctgc atccttggct gatcttagcc cggggcctcg
     3061 ggagccgccg gtcgttttcg gatcaccgag gcgggcaccg aggtggaaat cgaggtggaa
     3121 atcgaggtgg gaatcgaggc gagcgctcag gcgcggatcc gggcgagcag cgcacccagg
     3181 ttcttgcgcc gcatctcgat gcagaactcc cggatcagcc gggcgtggct cttggccatc
     3241 gccacgaacc gcatgccgag accggacgcc aggtggtcat cctggcgata gcggacctgg
     3301 gccgccgccc agatcgaatc gtcgacgccc ggcacctcga gctccagggc cacgatcatc
     3361 ccgggcggcg gggcgagcat cgagatcgcc gacacgctga gcccgctgtc gctcaggttg
     3421 gtggccaggc cgcgcaccgg gcggtcgtgg acgtacgacg tgaccatggt ctcgaacgga
     3481 atccggaagc cgagtctgcg atcctgcacg ggcacctctg ccgatgtagg ataaaagcct
     3541 accagcactg acgctggcgc ccaatttttc atccgttgcg cggtgaccgg cgaagcggac
     3601 ggggcgcggc ggggggccgg tagcagcagg ccgggcgatc tgcgaacgga ggaacttgac
     3661 ggggcgccca ccgtgcgcat cgtgtctagg gtcgaaccgc cacccgacag gagtagctct
     3721 gacatgccgg tgtacgagta cgagtgcaaa gcctgtggcc gcgacttcga gtaccagcag
     3781 cgaatgtccg atcccgacaa gaccacctgc gaggtgtgcg gaggcgcgct cgaccgcctg
     3841 atctcgcgca ccgcgttctc gctcaagggc ggcggctggt acaaggacct ctacgcctcg
     3901 cccaagcccg agaaggcgga caagagcgac aaggcggccg gcacggagag cgccaagagc
     3961 gacggcgcgg cggccaccac cgcgaagtcg gacggtgggt ccaccgccgg cagctcgagc
     4021 accagcaccg ccagcagctc gagcaccagc accgccagca gcggcagcag cagcggatcc
     4081 ggcagctcgt cggctgcggc cgccaaggcg acctgaagag gtctcgacca agcggaccgg
     4141 ggccgactcc ggccttcttg acattgtcct ggacgatgac cttgacgaga acgttgacgt
     4201 cgacggcaac gtaaacgtct gatcccaccc ttgaccttgc acgctgaccc cggaggtcga
     4261 tgtcgaggtc gacggtgggg gcaacgtcgt ccgtcgccgt acgtcgccgt caacctcaac
     4321 gttcgggtca aggtcgacgt caaaccgtca acatcgacca ccgctcgcgt cccggatcgc
     4381 ctcttccctt ttcactcgac agcgatccgc acttcggcag ctgaccgcgc cgcggcgtcc
     4441 tggtacacca tgcacgccat ggacgggcag cgatctgcga ttcacatcga tggcaaggcg
     4501 gtcgcggcgc gggtccgcgc cgaggtcgcc gacgcggccg ccg
//
LOCUS       DASVOR010000425         2759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_529948,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000425 DASVOR010000000
VERSION     DASVOR010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2759
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529948"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF516_08470"
     CDS             <1..292
                     /locus_tag="VF516_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107751.1"
                     /translation="ILVQHLNRPPPALAPRCPDAPYGLVELVERMLAKDPEERPKAAE
                     ISASLGKLRVARQQPPLRVTAEASNAVPLPVFEQTTTTPSTYLVPAWSDSEE"
     gene            complement(303..551)
                     /locus_tag="VF516_08475"
     CDS             complement(303..551)
                     /locus_tag="VF516_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107752.1"
                     /translation="MPATQESLDDLELPSYLPDDRDGEQPPHGPDDREHRVAHRPNPL
                     PAREEDEEELAEADILRELDFEEDDLDLDLDDLDRVDD"
     gene            complement(581..730)
                     /locus_tag="VF516_08480"
     CDS             complement(581..730)
                     /locus_tag="VF516_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107753.1"
                     /translation="MSSRALAMPPSPRLADIAPRILENVAGGLLELMIHVVEGWRDRR
                     DRIDR"
     gene            complement(858..1961)
                     /locus_tag="VF516_08485"
     CDS             complement(858..1961)
                     /locus_tag="VF516_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX8107754.1"
                     /translation="MTERIRLLVVDDDEVDRLAIRRAVEHSDLDAELREATTTDAARP
                     LLSQRFDCLLLDHDLPGVTGIELTRQLRAAGDLTPVILVTGAHDDDLLQTAVDAGITD
                     FIPKSDVSPRRIALRVRFAIRIGRAEAEAQRALAAATQAVRARDEVLAVVSHDLRGPL
                     QTIGFACDLLRSEVSGDGVRHLGAIERAMQNAERLIKDLLQASAIEAGGLTLERAPVD
                     AAAIVRQATADHELLAKENGGLITAKLPAGSTLVSADRDRVLQVLNNLIGNALKHAKG
                     APIEISLERNDGDAVIAVRDRGPGIADTELPHVFDRYWSGRTKKAGAGLGLAIAKGIV
                     DAHGGRIAVASKKGEGAEFSFTLPLAPPSTRSR"
     gene            complement(1958..2398)
                     /locus_tag="VF516_08490"
     CDS             complement(1958..2398)
                     /locus_tag="VF516_08490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107755.1"
                     /translation="MKQQRIHLLLVEDDDLDVMNVHRALSQAPEIASITVARDGIEAL
                     RLLRAGALALERLVILLDLRMPRMSGLDLLKELRADPRLKRMPTVVLTTSDDPHDRDA
                     AFCLGAAGYFVKPAAPGRFRQIMDAMRSYWSVAEFAPPPLEGKL"
     gene            complement(2419..>2759)
                     /locus_tag="VF516_08495"
     CDS             complement(2419..>2759)
                     /locus_tag="VF516_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8107756.1"
                     /translation="IVITTESPGELITLDEMERRYVRQVLNAVGGNKTHAARILGIDR
                     RSLYRRLEEPRHDPKAAESASAASHARTPVPSPPPAHASNGHIGGSNGHIGGSSGGSG
                     TAASHPGEVE"
BASE COUNT          367 a          981 c         1007 g          404 t
ORIGIN      
        1 gatcctcgtc cagcacctca accgcccgcc gccggcgctc gcgccgcgct gcccggacgc
       61 gccgtacggc ctggtcgagc tcgtcgagcg catgctcgcc aaggaccccg aggagcggcc
      121 caaggccgcc gagatcagcg cctcgctcgg caagctgcgc gtcgcgcgcc agcagccgcc
      181 cctgcgggtc acggccgagg cgagcaacgc tgtgccgctg ccggtgttcg agcagacgac
      241 gacgacgccg tcgacctacc tggtgccggc gtggtcggat tccgaggagt gacgcgggtc
      301 agctagtcgt cgacccggtc gaggtcgtcg aggtcgagat cgaggtcgtc ttcctcgaag
      361 tcgagctcgc ggaggatgtc ggcctcggcg agctcctcct cgtcttcctc gcgggcgggc
      421 agcgggttgg gccggtgggc gacgcgatgc tcgcgatcgt cggggccgtg cggaggctgc
      481 tcgccgtccc ggtcgtccgg caggtagctc ggcagctcga ggtcatcgag ggattcttgg
      541 gtggcaggca tgacgcgatc cgcagcaagc ggggtgccag tcagcggtcg atccggtcgc
      601 ggcggtcgcg ccagccctcg acgacgtgga tcatcagctc cagcagccca ccggcgacgt
      661 tttccaggat gcgcggcgcg atgtccgcca gccgcggcga cggcggcatg gcgagcgcgc
      721 gagacgacat gctcgtgacc atgatccgag gacaaagcac tgcgtgtgcc acacctcacc
      781 gccgtccagc gccgtgccgg tgcgcgggca ccacgccacg gccgtgcgcg tgggcccggc
      841 ggcgcgcgat cgcacgatca gcgcgatcgc gtcgacgggg gcgcgagcgg cagggtgaac
      901 gagaactcgg cgccctcgcc cttcttgctc gccaccgcga tccgcccgcc gtgggcatcg
      961 acgatgccct tggcgatcgc caggccgagg ccggcgccgg ccttcttggt ccggccgctc
     1021 cagtagcggt cgaacacgtg cggcagctcg gtgtcggcga tcccggggcc ccggtcgcgc
     1081 accgcgatca ccgcgtcgcc gtcgttgcgc tccagcgaga tctcgatcgg cgcgcccttg
     1141 gcgtgcttga gcgcgttgcc gatcaggttg ttgagcacct ggagcacgcg gtcgcgatcc
     1201 gcggacacca gcgtcgagcc ggcgggcagc ttcgcggtga tcaggccgcc attctccttg
     1261 gcgagcagct cgtgatcggc ggtcgcctgg cgcacgatcg cggcggcgtc gaccggcgcg
     1321 cgctccaggg tgagcccgcc ggcctcgatc gcgctggcct ggagcagatc cttgatcagg
     1381 cgctcggcgt tctgcatcgc gcgctcgatc gcgccgaggt ggcggacccc gtcgccgctg
     1441 acctcgctgc gcagcagatc gcaggcgaac ccgatggtct gcagcgggcc gcgcaggtca
     1501 tgcgacacca ccgcgagcac ctcgtcgcgg gcgcgcaccg cctgggtcgc ggcggccagc
     1561 gcgcgctggg cctcggcctc cgcgcggccg atccggatcg cgaaccggac gcgcagcgcg
     1621 atccggcgcg gcgacacgtc gctcttgggg atgaagtcgg tgatcccggc gtcgaccgcg
     1681 gtctgcagca ggtcgtcgtc gtgcgcgccg gtcaccagga tcaccggggt gaggtcgccg
     1741 gcggcgcgca gctgccgggt cagctcgatc ccggtgacgc cgggcaggtc atgatcgagc
     1801 agcaggcagt cgaagcgctg cgacagcagc ggccgcgccg cgtcggtggt ggtcgcctcg
     1861 cggagctccg cgtcgaggtc gctgtgctcg accgcgcggc gaatcgccag ccgatcgacc
     1921 tcgtcgtcgt cgaccaccag gagccggatc cgttcggtca tagcttgccc tcgagcggcg
     1981 gcggcgcgaa ctcggccacc gaccagtagc tgcgcatcgc gtccatgatc tggcggaacc
     2041 ggcccggggc ggccggcttc acgaagtatc ccgcggcgcc caggcagaac gcagcgtcgc
     2101 gatcgtgggg atcgtccgag gtcgtcaaca ccacggtggg catccgcttc aggcgcggat
     2161 cggcgcgcag ctccttgagc agatcgagcc ccgacatccg cggcatccgg aggtcgagca
     2221 ggatcacgag ccgctccagc gcgagcgctc ccgcacgcag cagacgcagc gcctcgatgc
     2281 cgtcacgcgc gacggtgatc gaggcgatct ccggagcctg ggacagtgcg cgatggacgt
     2341 tcatgacgtc cagatcgtca tcttccacca gcaggagatg gatccgctgc tgcttcatga
     2401 tccccgccga cgcccgcgct actcgacctc cccgggatga ctggccgcgg tcccggagcc
     2461 ccccgaggac ccgccgatgt gtccgttcga tccgccgatg tgcccgttcg acgcgtgcgc
     2521 ggggggcggg gacggtaccg gggttcgtgc atggctggcc gcgctggccg actcggccgc
     2581 cttcggatcg tggcgcggct cctcgagccg gcggtacagc gagcgccggt cgatgccgag
     2641 gatgcgcgcg gcgtgggtct tgttgccacc gaccgcgttg agcacctggc ggacgtagcg
     2701 gcgctccatc tcgtcgagcg tgatcagctc gccgggggac tcggtggtga tcacgatct
//
LOCUS       DASVOR010000426         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412495,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000426 DASVOR010000000
VERSION     DASVOR010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412495"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2719
                     /locus_tag="VF516_08500"
     CDS             <1..>2719
                     /locus_tag="VF516_08500"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8107757.1"
                     /translation="KRSGRLIVGKFEQYERNIPYFTITQALRELALDIVAEGELGIAR
                     WRQQLPQALGPHGKLIVDLVPQLGLVVGPQPLVPELSLTESETRLRRVFGRLFATCAT
                     PDHPLAMFIDDMQWADTASLALIASLLTDGNTRHLLIIGAYRDNEVDPTHPTMRTLAA
                     ARQGGARIRDVVLGPLSDGDLGRLVADTVQASPDEAAPLASLVRDKTGGNPFFAIQFL
                     TALHHKRSIWFDREAHRWQWDGARIRAEGYTDNIAELVRSRLYALPSETQAALQRAAC
                     IGGTVDAATLAIACEREVAPALRPAIEQHLLFETVQADRHTYRFPHDRMHEAAYTLLP
                     EPERARVHLAIGRRMLASMPPEELPGKVFEIVSQLDRGAALIDSTQERERLAELYLLA
                     GTRAQASTAYASALRYFTAGAELLAAGPEVCRAELAFALDLHRAECEFLTGALDAAEH
                     RLTELARHAASLVDLAALTSARIALYTTLDRAALAVEAMLEYLRRVKIDWPAHPTDDD
                     VRREYDRIWQQLGSRAIEELADLPPMTDPQHRATMDVLALAQSPVLFTDEKLHSLVIC
                     RMVNLSLEHGNSDGSGVGYACLGALLRPRFGNPAAGFRFGQVGLDLVEKRGLLRWKAR
                     VHLEFGFLITPWSKHLRGGIELVRRCFTAANETGDLMFASYSCNALVTLLLAVGDPLA
                     VVQREAETALAFVRKAKFGLVIDHLTAQLQLIRSLQGMTRKLGSLDDDGFAEDRFERH
                     LESDPHLVLATCWYWIRKLEARFLAGDHAEALAAAAKARPLLWAIASFPEAFEYVFYA
                     ALACAAYHDAAPDDDRPRLRDELAAHHAQLAAWAEHGPDNFGDRAELTGAELARIRGD
                     TDQAERLYEQAIRTARAHGFVHIEAIAYETAARFHRARGQTLIADAYVRE"
BASE COUNT          391 a         1005 c          937 g          386 t
ORIGIN      
        1 caagcggagc ggccggctga tcgtgggcaa gttcgagcag tacgagcgca acatcccgta
       61 cttcacgatc acccaggcgc tccgcgagct cgcgctcgac atcgtggcgg aaggcgagct
      121 cgggatcgcc aggtggcggc agcagctccc gcaggcgctc ggaccgcacg gcaagctgat
      181 cgtggatctg gtcccgcagc tcgggctggt cgtcggacca caaccgctgg tgcccgagct
      241 ctcgctgacc gagtccgaga cccggctccg ccgggtgttc ggccggctgt tcgcgacgtg
      301 cgcgacgccc gaccacccac tcgcgatgtt catcgacgac atgcagtggg cggacaccgc
      361 gagccttgcg ctgatcgcca gcctcctgac cgacggcaac acccgtcacc tgctgatcat
      421 cggggcctat cgcgacaacg aggtcgatcc gacgcacccg acgatgcgca cgctcgcagc
      481 ggcgcgacag ggcggcgcgc ggatccgcga cgtcgtgctc ggaccgctgt ccgacggcga
      541 tctcggccgg ctggtcgccg acacggtcca ggcctcgccc gacgaggcgg cgccgctcgc
      601 gagcctggtc cgcgacaaga ccggcggcaa cccgttcttc gcgatccagt tcctgaccgc
      661 gctccatcac aagcgctcga tctggtttga tcgcgaagca caccgctggc aatgggacgg
      721 cgcgcggatc cgcgccgagg gctacaccga caacatcgcc gagctggtgc gcagccggct
      781 gtatgcgctg ccgagcgaga cccaggcggc gctccagcgg gcggcgtgta tcggcggcac
      841 ggtcgatgcc gccacgctcg cgatcgcgtg cgagcgcgag gtcgcaccgg cgctgcgccc
      901 cgcgatcgag cagcacttgc tgttcgagac cgtccaggcc gaccggcata cctaccggtt
      961 cccccacgac cgcatgcacg aggcagccta cacgctactg cccgagccgg agcgcgcgcg
     1021 ggtccacctc gcgatcggcc ggcggatgct cgcgagcatg ccccccgagg agctccctgg
     1081 gaaggtgttc gagatcgtga gccagctcga ccgcggcgct gcgctgatcg actcgacgca
     1141 ggagcgcgag cggctcgccg agctgtacct gctcgccggc acgcgcgcgc aggcctcgac
     1201 cgcctacgca tccgcgctcc gctacttcac cgcgggtgcc gagctgctcg cggcgggccc
     1261 cgaggtctgc cgcgccgagc tcgcgttcgc gctcgacctc caccgcgccg agtgcgagtt
     1321 cctgaccggc gcgctcgacg cggccgagca ccgactcacc gagctcgcgc gccacgccgc
     1381 gagcctggtc gacctggcgg cgttgacgtc ggcgcggatc gcgctctaca cgacgctcga
     1441 tcgggcggcg ctcgccgtcg aggcgatgct cgagtacctg cggcgcgtga agatcgactg
     1501 gccggcacac ccgaccgacg acgacgtccg gcgcgagtac gatcggatct ggcagcaact
     1561 cgggtcgcgc gcgatcgagg agctggccga cctgccgccg atgaccgatc cgcagcaccg
     1621 cgcgacgatg gacgtgctcg cgctcgccca gtcgcccgtg ctgttcaccg acgagaagct
     1681 gcactcgctc gtgatctgcc ggatggtgaa cctgagcctc gagcacggca acagcgatgg
     1741 ctcgggcgtc ggctacgcct gtctcggcgc gctcctccga ccgcggttcg gcaaccccgc
     1801 ggcgggcttc cggttcggcc aggtcgggct cgatctggtg gagaagcgcg ggctcttgcg
     1861 ctggaaggcg cgcgtccacc tcgagttcgg gttcctgatc acgccgtggt cgaagcacct
     1921 gcgcggcggc atcgagctgg tgcggcgctg cttcacggcg gcgaacgaga ccggcgatct
     1981 catgttcgcg tcgtactcct gcaacgccct ggtcacgctc ctcctcgcgg tgggcgatcc
     2041 gctcgcggtg gtgcagcgcg aggccgagac cgcgctcgcg ttcgtgcgca aggccaagtt
     2101 tggcctggtg atcgaccacc tcacggcgca gctccagctg atccgctcgc tgcaggggat
     2161 gacccggaag ctgggctcgc tcgacgacga cggcttcgcc gaggaccggt tcgagcgtca
     2221 cctcgagtcg gatccgcacc tggtgctcgc cacgtgctgg tactggatcc gcaagctgga
     2281 ggcacgcttt ctcgccggcg accacgccga ggcgctggcg gccgcggcca aggcgcggcc
     2341 gctgctgtgg gcgatcgcgt cgttccccga ggcgttcgag tacgtgttct acgccgcgct
     2401 cgcgtgcgcc gcctatcacg acgccgctcc cgacgacgac cgcccccggc tgcgcgacga
     2461 gctcgccgcg caccatgcgc agctcgcggc ctgggcggag cacggtcccg acaacttcgg
     2521 cgaccgcgcc gagctcaccg gtgccgagct cgcgcggatc cgcggcgaca cggaccaggc
     2581 ggagcggctc tacgagcagg cgatccgcac cgcgcgcgca cacggcttcg tccacatcga
     2641 ggcgatcgcc tacgagacgg cggcgcggtt tcatcgcgcg cgcggccaga cgctgatcgc
     2701 cgacgcctac gtgcgcgag
//
LOCUS       DASVOR010000427         1893 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_529994,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000427 DASVOR010000000
VERSION     DASVOR010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_529994"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VF516_08505"
     CDS             complement(<1..951)
                     /locus_tag="VF516_08505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107758.1"
                     /translation="MEQFGKYSLIRKIGTGGMAEVFLARTTVAQGLNKTLVIKKIHTA
                     YARSRQFVAMFVDEAKIALGLNHPNITQVFDFGAVADTYFLAMEYVEGIDLLRLLQEA
                     ARARQRLPYGVSAYIVQQLAKGLDYAHRKTDEFGQPLGIVHRDVSPQNILLSWDGSVK
                     IVDFGIARARDVHEEEGVIKGKYAYMSPEQARGEPVDCRSDVFAAGIVLFELVCARPL
                     FHGKGKDALEMVKSGVLPRPRDHAPDLPDALERIIARALAFRREDRFATARDLQHELG
                     KFQLAWGHKIGALIESGSLAQTLAQLVPHERASAPRPPAEP"
     gene            complement(1056..1676)
                     /locus_tag="VF516_08510"
     CDS             complement(1056..1676)
                     /locus_tag="VF516_08510"
                     /inference="COORDINATES: protein motif:HMM:NF023669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107759.1"
                     /translation="MQRWLAIGLVLAACKVREQPPITAPWTWDFDAGLGDVFYATGDG
                     YRGERGALSAHGAHNHPLWLRKKLPRDVRIEFDAWSTEQRGDLKVEVFGDGHSFDPDG
                     GRYTATGYEVIFGGWFNSKSIIARLDEHGTEIAERTEPKVVPGRHYHWRIERRGKAIT
                     WFIDDLATPFLRYEDPRPLDGAGHEYFGFNNWETDTWFDNLVIAPL"
BASE COUNT          283 a          636 c          669 g          305 t
ORIGIN      
        1 gggctcggcc ggaggccgcg gcgcgctcgc gcgctcgtgc gggaccagct gcgcgagggt
       61 ctgggcgagc gatccggact cgatcagcgc gccgatcttg tggccccacg cgagctggaa
      121 cttgccgagc tcgtgctgca ggtcgcgcgc ggtcgcgaac cgatcctcgc ggcggaacgc
      181 gagcgcgcgc gcgatgatgc gctccagcgc gtcgggcagg tccggcgcgt ggtcgcgcgg
      241 ccgcggcagc acgcccgact tgaccatctc gagcgcatcc ttgcccttgc cgtgaaacag
      301 cggccgcgcg cacaccagct cgaacagtac gatgccggcg gcgaacacgt cgctgcggca
      361 atcgaccggc tcgccgcgcg cctgctccgg cgacatgtac gcgtacttgc ccttgatcac
      421 gccctcctcc tcgtgcacgt cgcgcgcgcg ggcgattccg aagtcgacga tcttcacgct
      481 gccgtcccat gacagcagga tgttctgggg cgacacgtcg cggtggacga tgccgagcgg
      541 ctggccgaac tcgtcggtct tgcggtgcgc gtagtcgagg cccttggcga gctgctgcac
      601 gatgtacgcc gatacgccgt agggcaggcg ctggcgggcc cgggcggcct cctgcagcag
      661 gcgcaacagg tcgatgccct cgacgtactc catcgccagg aagtaggtgt cggcgaccgc
      721 gccgaagtcg aacacctggg tgatgttcgg gtggttgagg ccgagcgcga tcttcgcctc
      781 gtcgacgaac atcgcgacga actggcggct gcgcgcgtac gcggtgtgga tcttcttgat
      841 caccagcgtc ttgttgagcc cctgcgccac ggtggtccgg gccaggaaga cctcggccat
      901 gccgcccgtc ccgatcttgc ggatcagcga gtatttgccg aactgctcca tgtcggggga
      961 tcaaccgggc caacgacggg gccaaagatg agatcgaacg acgcgaacac cggcgcgtgg
     1021 agatcaccgg cgagccgggc cgcgccattg tagcgtcaca gcggcgcgat cacgaggttg
     1081 tcgaaccagg tatcggtctc ccagttgttg aagccgaaat actcatggcc ggcgccgtcg
     1141 agcgggcgcg ggtcctcgta gcgcaagaac ggcgtcgcga ggtcgtcgat gaaccacgtg
     1201 atcgccttgc cgcggcgctc gatccgccag tggtagtgcc ggccgggcac gaccttgggc
     1261 tcggtgcgct cggcgatctc ggtgccgtgc tcgtcgagcc gcgcgatgat cgacttcgag
     1321 ttgaaccagc cgccgaagat cacctcgtag ccggtcgcgg tgtagcggcc gccgtcgggg
     1381 tcgaacgagt ggccgtcacc gaacacctcg accttgaggt cgccgcgctg ctcggtcgac
     1441 cacgcgtcga actcgatccg gacgtcgcgc ggcagcttct tgcgcagcca cagcggatgg
     1501 ttgtgtgcgc cgtgcgccga cagcgcgccg cgctcgccgc ggtagccgtc gccggtggca
     1561 tagaacacat cgccgagccc ggcgtcgaaa tcccaggtcc acggcgcggt gatcggcggc
     1621 tgctcgcgca ccttgcaggc ggcgagcacg aggccgatcg cgagccagcg ctgcatcgag
     1681 cccgtatcgt acgcgatcgg gcgatctcgg tgaatcctaa cgtattcgat cgcttgcgat
     1741 cttcggcggt ccccgaaggc gattcggtgt cagaccgctg ccctagcctg gcagcgtgga
     1801 aggtacgctg tcgctgttcg actggcgccc ggcgcccgtg atgccggacg ctcccgggcg
     1861 catgccggag cctgtcgagg aggtggccgc ggg
//
LOCUS       DASVOR010000428         5586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_62457,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000428 DASVOR010000000
VERSION     DASVOR010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5586
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62457"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1129)
                     /locus_tag="VF516_08515"
     CDS             complement(173..1129)
                     /locus_tag="VF516_08515"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX8107760.1"
                     /translation="MSLRVSLVVPIAAALALTAAPARAHEMTMAEMEVRETSPGEFLW
                     QWTASSDKRPMGNDLVPHWPEGCSTGPNAVRCGSTGLTGTLAIEGVGQRYSAAMVKVI
                     WLDGQTRVYTFTGAQPSMQLYGSADDRRGTAEIARAYVVLGVQHILSGIDHLLFVVGL
                     LFLVGFRRRLVGTITAFTLAHSLTLACSVFGWLTLRPAPVEAMIAMSIVLVACEALRD
                     HETLARRMPALVSFLFGLVHGLGFAGALRSIGLPQRHLPLALFTFNVGVEIGQLMTVL
                     AAYVVVRLLLAKRWFARARTPALYAIGVVAAYWCWQRIALLI"
     gene            complement(1126..2040)
                     /locus_tag="VF516_08520"
     CDS             complement(1126..2040)
                     /locus_tag="VF516_08520"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8107761.1"
                     /translation="MIAPLADNPASDHVTASEHAAPDAVASPRRARRHRPAWLREPLL
                     HFVVLGGVLFGADHFLADRGADPHTIIVGADVDGEAHETFKATRGREPNAQELEALHR
                     VWLDNEVLYREGLALQLDKGDSAIRERVIFKALTMIDSNVHVPTADDKVLRAWFDGHR
                     DKYDEPARYSFEDAALSGDSSEAAVRKFVTALNSGVPGDAKAGLRVFKDRPQASLVES
                     YGPEFVKTLARAQPDTWQAVRTGDGWRAIRIDAITPAKPAVFEVVRGMVQHDWIDATA
                     AEQRTAAVRALAKKYTIKYEPPTRDDLE"
     gene            complement(2048..5194)
                     /locus_tag="VF516_08525"
     CDS             complement(2048..5194)
                     /locus_tag="VF516_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107762.1"
                     /translation="MNGVGNRPNTSSIAGRLSPGIARRVHLQIRHAIVTWPRCRVRFR
                     DPRAAARPISLKTPSKRCRSVAVHHGGALYECRAWPGTSAPARRRGGRLRRASVPQQA
                     RLGGFAIMSILEKMTMLALGICSVGSLTGCGDNGANAESPSGTGEIGLSLQIPNGTVI
                     ASASYSITGPMGFIKNGVIDTSGSSTLSTLIGGIPAGAGYQISILAATSDGATTCTGT
                     TSFDVMARLTTSAVVPMTCLEKPRTGSVLVNGRLNICPTIDGISANPAEAEVGGTIAL
                     SAAAHDSDAGPSPLSFGWTSTAGSLSDAAAKNPTFTCTTPGTVTLHLSVGDGDPAAAC
                     AGASTVQVTCSLAPKTPGTYVAGDFHNHTTCSDGSISMAKLVKKATDRVDTPWGLDWF
                     VQAGHGGNGSRNCTLVEDATLTTPAYPFGAGVGPNTSWENSGITPKGDVSGTSPNHNM
                     WRWQSIQEFQYPLIEYLNTFKNLPLFLGIETVAPGHEHTSMSIITGQIPATLDAGNLP
                     AAPPYTALGNGTALAQWEYCFDRADTDTSRGAGNSWDCSVPGSLNAADPSWNATAQKL
                     IPAGGAGNGTRGHSKTLEALRWMATFHADASYYVPAHLERAGQFNPDGNNGFNVESLR
                     DFNNAAPRIAFGFETQPGHGAAAARGEYQRLRNNIGGVQTDSVGGTTYGGTGVYGGVI
                     GGVWDALLGEGRRWWFFASSDWHNRGSFGPDDRRSTGDFYPGEYQRNYTMVRSGGNKP
                     RPQTIVDGLRSGNNFATGGQIIDRLAFVVCTSRPDALVAELVANAAVSNTALGAAGCA
                     TMGEKLVVPPGSDLVVGIAVRDPAGPNFSPYTFFNPSLLQVGISQPINMPVLDHIDLI
                     GGLVTGFKVPGTPGYAGEWPRNTSWLHADGTVADLSVVPDAAKNTSAAILRTFNGNGA
                     SPWTPVTSQFDGSTFLTMTFRIPAVTASQYVRLRGTNLPPAVPFETDPNGNPLPDIFT
                     NANDPTMLRIPCTIAHSASSQFDGCPDHLATATGTDNPIVGQRAVSFDVAAWSDLWFY
                     SNPVYIEVTGSVVVAGVQ"
     gene            5157..>5586
                     /locus_tag="VF516_08530"
     CDS             5157..>5586
                     /locus_tag="VF516_08530"
                     /inference="COORDINATES: protein motif:HMM:NF025134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEX8107763.1"
                     /translation="MDEVFGLFPTPFMRAPGTLGQPLVARLIEHFTARAIRDNNSSGN
                     LVHTEMLRPQDSPLLVEAAGLIAPKLVDFGTHLFGERLPWLLKEMWVNVLDTGGRQAM
                     HNHANSFISGVVYLTRTHPSSQTVFMKSPGGTDFLFKNDHK"
BASE COUNT          813 a         1866 c         2015 g          892 t
ORIGIN      
        1 gcgccgtgct ccgcgtgagc cgggcgcggc tggccgagct caacgcccgg ctcgggatcg
       61 gatagctccg ggggccgcgc ggcaggaccg ctcgcctcgg ccggcgtcac gacgccgcga
      121 gcagggcgtg ccagtcgtcg accgagagca cctggtgctg cggcatgatc ggtcagatca
      181 gcaacgcgat gcgctgccag caccagtagg ctgccaccac gccgatcgcg tacagcgccg
      241 gagtccgcgc ccgggcgaac cagcgcttcg cgagcagcag gcgcacgacc acgtaggcgg
      301 ccaggacggt catcagctgg ccgatctcga cgccgacgtt gaaggtgaac agcgccaggg
      361 ggagatggcg ctgcggcagg ccgatgctgc ggagggcgcc tgcgaacccc agcccgtgga
      421 ccaggccgaa caggaacgag accagcgccg gcatgcggcg agccagcgtc tcgtggtcgc
      481 gcagcgcctc gcaggccacc agcacgatcg acattgcgat catggcctcg accggcgcag
      541 gtcgaagcgt cagccatccg aacacgctgc acgcgagcgt caggctgtga gccagcgtga
      601 acgcggtgat cgtgccgacc agccggcgcc ggaagccgac gaggaacagc agaccgacga
      661 cgaacagcag gtggtcgatg ccgctcagga tgtgctggac gccgagcacg acgtacgccc
      721 gcgcgatctc cgcggtaccg cggcgatcgt ccgccgatcc gtagagctgc atcgatggct
      781 gcgcgcccgt gaacgtgtag acccgggtct ggccgtcgag ccagatcacc ttcaccatcg
      841 ccgcggagta gcgctggccg acgccctcga tcgccagcgt gccggtgagg cctgtgctgc
      901 cgcagcgcac cgcgttcggg ccggtcgagc agccctccgg ccagtgcggg acgagatcgt
      961 ttcccatcgg gcgcttgtcg ctcgaggccg tccattgcca caggaactcg cccggggagg
     1021 tctcccggac ctccatctcc gccatggtca tctcgtgggc ccgcgccggc gcggccgtga
     1081 gcgccagcgc ggcggcgatc gggaccacga gcgagacacg caggctcatt cgagatcgtc
     1141 ccgggtcggc ggctcgtact tgatcgtgta cttcttcgcc agcgcgcgca cggcggcggt
     1201 ccgctgctcc gcggcggtgg cgtcgatcca gtcgtgctgc accatgccgc gcaccacctc
     1261 gaacacggcc ggcttcgcgg gggtgatcgc gtcgatccgg atcgctcgcc agccgtcacc
     1321 ggtccgcacc gcctgccagg tgtccggttg cgcgcgggcc agggtcttga cgaactccgg
     1381 cccgtagctc tcgaccaggc tggcctgcgg ccgatccttg aacacgcgca ggccggcctt
     1441 cgcgtcgccc ggcactccgc tgttgagcgc cgtcacgaac ttgcgcaccg cggcctccga
     1501 gctgtctccc gacagcgcgg cgtcctcgaa cgagtagcgc gcgggctcgt cgtacttgtc
     1561 gcgatggccg tcgaaccacg cgcgcagcac cttgtcgtcg gccgtcggga catggacgtt
     1621 gctgtcgatc atggtgagcg ccttgaagat gacgcgctcg cggatcgcgg agtcgccctt
     1681 gtcgagctgc agcgccagac cttctcgata cagcacctcg ttgtcgagcc acacgcggtg
     1741 cagcgcctcg agctcctgcg cgttcggctc gcgtcctcgg gtcgccttga acgtctcgtg
     1801 tgcctcgccg tcgacgtcgg ccccgacgat gatcgtgtgc gggtcagcgc cgcggtccgc
     1861 gaggaagtgg tcggcaccga acagcacgcc accgagcacg acgaagtgca ggagcggctc
     1921 gcgcagccag gccggccggt gccggcgagc gcgtcgcggt gatgcgaccg cgtccggcgc
     1981 ggcgtgctcg gacgctgtca cgtggtcgga ggcggggttg tcggccagtg gcgcgatcat
     2041 gggaccgtca ctgcacccct gcgacgacga ccgagccggt cacctcgatg tagaccgggt
     2101 tgctgtagaa ccacagatcc gaccacgcgg cgacgtcgaa cgataccgcg cgctggccga
     2161 cgatcgggtt gtccgtcccg gtcgccgtcg cgaggtggtc cgggcagccg tcgaactggc
     2221 tgctcgcgct gtgggcgatg gtgcacggga tacgcagcat cgtcggatcg ttcgcgttgg
     2281 tgaagatgtc gggcagcggg ttgccgttgg ggtcggtctc gaacggcacc gcgggcggca
     2341 ggttggtccc gcgcagccgg acgtactgcg aggcggtgac cgcggggatg cggaacgtca
     2401 tggtcaggaa cgtcgagccg tcgaactgcg aggtcaccgg ggtccagggc gaggcgccgt
     2461 tgccgttgaa cgtgcggagg atcgcggcgc tggtgttctt ggccgcatcg gggacgaccg
     2521 agaggtccgc gaccgtgcca tcggcatgca gccagctggt gttgcgcggc cactcaccgg
     2581 cgtagccggg ggtgcctggc accttgaagc cggtgaccag tccaccgatg agatcgatgt
     2641 ggtcgagcac cggcatgttg atcggctggg agatgccgac ctggagcagc gacgggttga
     2701 agaaggtgta cggcgagaag ttcgggccgg ccggatcgcg caccgcgatc ccgacgacga
     2761 ggtccgaccc gggtggcacc accagcttct cgcccatcgt ggcgcagccc gccgcgccga
     2821 gcgccgtgtt gctgaccgcc gcattggcga ccagctcggc gacgagtgca tcggggcggc
     2881 tggtgcacac cacgaacgcg agccgatcga tgatctggcc tccggtcgcg aagttgttgc
     2941 cgctgcgcag gccgtcgacg atggtctgcg gcctcggctt gttcccgccg ctgcggacca
     3001 tggtgtagtt gcgctggtac tctccggggt agaagtcgcc ggtcgagcgg cggtcgtccg
     3061 ggccgaagct gccgcggttg tgccagtccg aactcgcgaa gaaccaccag cggcggcctt
     3121 cgccgagcag ggcatcccac acgccgccga tcaccccgcc gtagaccccc gtaccgccat
     3181 aggtcgtgcc gccgacgctg tcggtctgga caccaccgat gttgttgcgg agacgctggt
     3241 actcgccgcg cgccgcggcg gcgccgtggc cgggctgggt ctcgaatcca aatgcgatcc
     3301 tcggggccgc gttgttgaag tcgcgcagac tctcgacgtt gaatccgttg ttgccgtcgg
     3361 ggttgaactg gccggcgcgc tcgaggtggg cggggacgta gtagctggcg tcggcatgga
     3421 acgtggccat ccacctgagc gcctcgagcg tcttgctgtg gcccctggtg ccgttaccgg
     3481 cgccgccggc ggggatcagc ttctgcgcgg tggcgttcca gctcggatcc gcggcgttca
     3541 ggctgccggg aaccgagcag tcccagctgt tgccggcgcc gcggctggtg tccgtgtcgg
     3601 cgcgatcgaa gcagtactcc cattgcgcga gcgcggtgcc gttgccgagc gcggtgtacg
     3661 gcggagcggc ggggagattt cccgcgtcga gcgtcgccgg gatctgaccg gtgatgatcg
     3721 acatggaggt gtgctcgtgg ccgggggcga cggtctcgat gccgaggaac agcgggagat
     3781 tcttgaacgt gttgagatac tcgatgagcg gatactggaa ttcctggatc gattgccacc
     3841 gccacatgtt gtggttcggg ctggtgcccg acacgtcgcc tttgggcgtg atcccggagt
     3901 tctcccacga cgtgttcggt ccgacgccgg cgccgaacgg gtaggcgggc gtggtgaggg
     3961 tcgcgtcctc gaccagcgtg cagttgcggc tgccgtttcc gccgtgcccg gcctgcacga
     4021 accagtcgag cccccagggc gtgtccaccc ggtcggtggc cttcttgacc agcttcgcca
     4081 tcgagatcga gccgtcggag cacgtcgtgt ggttgtggaa gtcgcccgcg acgtaggtgc
     4141 cgggggtctt cggcgccagt gagcacgtga cctggacggt cgacgcgccg gcgcacgcgg
     4201 cggcggggtc gccgtcgccg acgctgaggt gcagcgtcac ggtgcccggg gtcgtgcagg
     4261 tgaaggtcgg gttcttggcc gcggcgtcgc tgaggctgcc cgcggtggac gtccagccaa
     4321 acgacagcgg gctggggccg gcgtcgctgt cgtgcgcggc ggccgacagc gcgatcgtgc
     4381 cgccgacctc ggcctcggcc gggttcgccg agatcccgtc gatcgtcggg cagatgttca
     4441 gccggccgtt gaccagcacg ctgccggtgc gcggcttctc gaggcaggtc atcggcacga
     4501 cggccgaggt ggtcaggcga gccatcacgt cgaacgacgt cgtgccggtg caggtggtcg
     4561 ccccgtcgct ggtcgccgcg aggatcgaga tctggtatcc cgcccccgcc gggatgccgc
     4621 cgatgagggt gctcagcgtc gatgacccgg aggtatcgat cacgccgttc ttgatgaacc
     4681 ccatcggccc cgtgatggaa tagctcgcgc tggcgatcac ggtgccgttc gggatctgca
     4741 gcgacagacc gatctcgccg gtcccgctgg ggctctcggc gttcgcgccg ttgtcgccgc
     4801 atccggtgag ggagccaaca ctgcagatgc cgagggccag catcgtcatc ttctcgagga
     4861 tgctcatgat cgcgaagcct ccaagtcgtg cctgttgggg aacggacgcg cggcggagcc
     4921 ggcctccgcg ccggcgtgcc ggggcggaag tccccggcca cgcccggcac tcgtacaatg
     4981 cgccgccgtg atgcacggca acgcttctgc aacgtttgct tggcgttttc agcgagatcg
     5041 gacgtgcggc tgcgcgcgga tcgcggaagc ggacacggca tcgcggccag gtgacaatcg
     5101 cgtggcgaat ttggagatgg acacgcctgg cgatgccggg agataaccgg cccgcgatgg
     5161 acgaggtgtt cggcctgttc cccacgccgt tcatgcgcgc gccgggcacg ctcgggcagc
     5221 cgctcgtcgc gcgcctgatc gagcacttca cggcccgcgc gatccgcgac aacaactcgt
     5281 cgggcaacct ggttcacacc gagatgctgc ggccgcagga cagcccgctg ctcgtcgaag
     5341 cggccggcct gatcgcaccc aagctggtcg acttcggcac ccacctgttc ggggagcgcc
     5401 tgccctggct gctcaaggag atgtgggtca acgtgctgga caccggcggc cgtcaggcca
     5461 tgcacaacca cgccaacagc ttcatctccg gggtggtcta tctcacgcgc acgcatccga
     5521 gctcgcagac cgtgttcatg aagtcgccgg gcggcacgga cttcctgttc aagaatgacc
     5581 acaagg
//
LOCUS       DASVOR010000429         4354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412540,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000429 DASVOR010000000
VERSION     DASVOR010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412540"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2529)
                     /gene="oxlT"
                     /locus_tag="VF516_08535"
     CDS             complement(<1..2529)
                     /gene="oxlT"
                     /locus_tag="VF516_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEX8107764.1"
                     /translation="MKRDELLSRLEKLLPAQFEGVLFHLQIPSAQLAGASAPQSIRAI
                     ELLRHLENTDELARLEPLLLSYDGAAGASRVSAQPPTATKPVPIYPDAEVEVLSKRLE
                     NARARNKKLRDSGIKADDLDREILELRRQLREGGQLRAGDALSDGRYLLVRVVGRGGF
                     AVVWEAFDCAEQQRVAIKVLHSNLAGDSLRRERFFRGAHAMMKLVHPAVVRVLEPKAE
                     DGGFYYFVMEFVADGNVREWVLRSRLKNEEILPLILRIGEALALAHSQGMIHRDIKPA
                     NILLDGHGAAKLTDFDLVGAHDTTGGTRTGALGTVVYAAPECLERPQEATACADVYGL
                     GMTAIFCLAGRDLSMDTFRNPDGTIDMLPCSIQIQAVLRRAVAWEPRERFADAAAMVL
                     ELREAMDPPGHDATALVSPAGADLPAPVPGPAIVSQPVVTQAVVSQAVVSRAVEAGFS
                     SVEAGSASVKGAHERMKVGRWWQLTFGIICMVMIANLQSSWMLFATLTRHWDRAQIQT
                     AFLIFAFVENCLAPVGGWLVDRFGPRAVVVTGSVLCALGWEKSAYGNSPTFLYVAWGI
                     AGIGASAVYGTCIGNALKWFPERRGLAVGITGSGFGAGAMATVLLQWASESYGSEATF
                     LWFGLGQGAVLFLIGLLLSHPDANAVAALPKPSSMVTHQPQFTSRQMLKTPVFWVMYG
                     MFVLMGAGGLFITANLKQIAVAFRVSDVPVVFLGLGPTAITLALVLDKIMNGFTRPFF
                     GWVSDRIGRENTMVIAFGIETIGVLMLSQLGDDPVMFVVLSGVVFFAWGEIYSLFPST
                     SADTYGWKHAAANAAWLYTAKGAAAFFVPLAVLVAKWASWHAVFM"
     gene            3296..>4354
                     /locus_tag="VF516_08540"
     CDS             3296..>4354
                     /locus_tag="VF516_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8107765.1"
                     /translation="MGAGLGGLVLARILRRHGVASTVYELDAGPDARRQGGLLDLHVG
                     SGQRALHDAGLLEQFRANTLPQGEHVRVMDKAGTVFIDRGPEGGEGLRPEIDRTVLRN
                     LLLASLPPGAVAWGHKLTAAERAEGGGFHLAFSDGRTTWAELLVGADGAWSRVRPLLS
                     AAVPEYVGITYLEFELTDVATRHPELARLVGTGSLFALSDNKNIGGRGGGHLHLGAGL
                     RVPEDWVRSSGVDWADPDAARAALLAEFADWSEGLKDLIRHCDDRITPRLIHALPIGH
                     SWSRGRGVTLLGDAAHLMSPFAGEGANAAMLDAAVLAGFLLEHEDDVETALARYEADM
                     FPRAAEAARQSAFGLDLIF"
BASE COUNT          717 a         1511 c         1416 g          710 t
ORIGIN      
        1 catgaacacg gcgtgccagc tcgcccactt cgcgacgagg accgcgagcg gcacgaagaa
       61 cgccgcggcc cccttggcag tgtacagcca ggctgcgttg gcagcggcgt gcttccagcc
      121 gtaggtgtcc gcgctggtcg acgggaacag gctgtagatc tcgccccagg cgaagaacac
      181 gacgccactc agcaccacga acatcaccgg atcgtcgccg agctggctga gcatcaggac
      241 gccgatcgtc tcgatcccga acgcaatgac catcgtgttc tcgcggccga tccggtcgga
      301 cacccagccg aagaacggcc gcgtgaagcc gttcatgatc ttgtcgagga ccagcgcgag
      361 cgtgatcgcc gtcggcccca gcccgaggaa gacgaccggc acatcggaga ccctgaaggc
      421 caccgcgatc tgcttgaggt ttgcggtgat gaacaggccg ccggcgccca tcaggacgaa
      481 catgccgtac atgacccaga acaccggcgt cttcagcatc tgccgcgaag tgaactgggg
      541 ctgatgggtc accatgctgg acggctttgg cagcgccgcc accgcgttgg cgtcagggtg
      601 agacagcaac aggccgatca ggaagagcac ggcgccttgg ccgaggccga accacaggaa
      661 cgtcgcctcg gagccgtacg actcgctggc ccactgcagc aggacggtgg ccatcgcccc
      721 ggcgccaaag cccgacccgg tgatgccgac tgccagcccg cgccgctccg ggaaccactt
      781 gagggcgttg ccaatgcagg tgccgtacac cgcgctcgcg ccgatccccg cgatgcccca
      841 cgcgacgtac aggaacgtcg gcgagttgcc gtacgcactc ttctcccagc ccagagcgca
      901 caacacgcta ccggtgacga cgaccgcgcg cggaccgaac cgatccacca gccagccccc
      961 aaccggggcc aggcagttct cgacgaacgc gaagatgagg aacgcggtct gaatctgcgc
     1021 cctgtcccag tggcgggtca acgtggcgaa cagcatccag ctggactgga ggttcgcgat
     1081 catgaccatg cagatgatgc cgaacgtcag ctgccaccag cggccgacct tcatgcgctc
     1141 gtgcgcgcct ttgaccgagg cagatcccgc ttcgacggaa gaaaaccccg cttcaactgc
     1201 ccgtgacacg actgcctgtg acacgactgc ctgtgtgacg acgggctgtg acacgatcgc
     1261 tggccccggg acgggcgcgg gcaggtccgc acccgccggg gatacgagcg ctgtcgcgtc
     1321 gtgtccggga ggatccatcg cctcccggag ctcgagcacc atggcagcag catcggcaaa
     1381 tcgctcgcgt ggttcccatg ccacggcccg ccggagcacg gcctgaatct ggatcgagca
     1441 ggggagcatg tcgatcgtcc cgtccgggtt ccggaacgtg tccatcgaga ggtctcgtcc
     1501 agccagacag aagatcgcgg tcatccccag tccgtagaca tccgcgcatg cagtcgcttc
     1561 ttgtggtctc tcaagacact ccggtgccgc atacacgacg gtaccgagcg cgccggtacg
     1621 agttccgccg gttgtgtcgt gcgcgccgac gagatcgaag tcggttagct tcgcggcgcc
     1681 atgcccgtcg agcaggatgt tggctggttt gatatcgcgg tggatcatgc cctgcgaatg
     1741 agccagcgcg agcgcttcgc caatccgtag gatgagtggc aagatctctt cgttcttcag
     1801 cctgctgcga agtacccatt ctcgtacgtt tccatcggcg acaaactcca tgacgaagta
     1861 gtaaaatccg ccgtcctcgg ctttgggctc gaggacgcgc accaccgcgg gatgcaccag
     1921 cttcatcatt gcgtgcgcgc cgcggaagaa tcgctcgcgg cggagcgaat cgccggcgag
     1981 attcgagtgc aataccttga ttgcgacgcg ctgctgctcc gcgcagtcaa atgcctccca
     2041 gaccacggcg aagccgcccc gacctacgac cctcacaaga agatagcgac cgtcgctcag
     2101 ggcgtcccca gcacggagct gtcctccttc gcggagctgt cttcgaagct caaggatctc
     2161 gcggtcgagg tcatcggctt tgatgccaga gtcgcgcagc tttttgttcc gtgcacgggc
     2221 attctcgagc cgcttgctga gcacctcgac ctcggcatcc ggatagattg gtaccggttt
     2281 cgtcgctgtc ggtggttgcg ccgagactct gctcgccccc gcggcgccgt cgtaggacag
     2341 gaggagtggc tcgagccgtg caagctcgtc ggtgttctcg agatgacgca gcaattcgat
     2401 ggcgcgtatg ctctgcggcg cactggcacc cgcgagttgc gccgacggga tctgcaagtg
     2461 aaacagcact ccctcgaact gcgccggtaa tagtttctca agccgcgata gcagctcatc
     2521 ccgcttcatc gactaccata cctgctgaag cccccttcgt cggcttgtgc ttgcgtagct
     2581 cgccgagcaa tccggtggtc gcaccgccca gcgcgtcacg agcttgaact cgttcccgca
     2641 agagccgagt tccttcgcgg gctgcgcctc gcgcagggca tcgccgatcg cgagagcacg
     2701 atgccgatgc tcgccagcgt cttgctgcgc atctgggcaa gcacccgcta cgctcaaggt
     2761 cgtcccggag cgggcgtgct ttgctatcga gcgcaagctc tgtggtgcca agcgggctgg
     2821 cagcaaggaa caggatgatg tgcttgctca acgacgacct cgaccttgct cgcgttggtc
     2881 cggttggacc gtcgatcatc aggcgacggg cctgcaactt tacgacatcg ccgtcgtgcg
     2941 gtgccgctgt gcacgtgccg cgaaccgcgg cagcccctcg aagtagatga caatgacttc
     3001 atcgtcttag cgcgacgaaa gcgggtcatg gcggctacca gcgacggcgt ggccgagcac
     3061 atcgcgatcg ccgcgatcgc ctggtgcagc gcgctgctgg cgattctcgt cgcctggtac
     3121 cggctcgggc cgctgcgcgc gctcgccgcg ctcgtgctgg tggtcgtgag ctggttcggc
     3181 tccgggtggt gctcgccacg agtcagacga gcacagaaga cgtcaccagc cggagctagc
     3241 tgtacacttc cttcgaccgc cgggagcccc catgtcctcc ccccgcatcg ccattgtggg
     3301 cgccggcctc ggcggtctgg tcctggcgcg catcctgcgc cggcacggcg tcgcctccac
     3361 cgtctacgag ctggacgccg ggccggacgc gcgccgccag ggcggcctgc tggacctgca
     3421 cgtcgggtcc ggccagcggg ccttgcacga cgccgggctc ctcgagcagt tccgggccaa
     3481 caccctgccg cagggcgagc acgtgcgggt gatggataag gcgggcaccg tcttcatcga
     3541 ccgcggccca gagggcggcg agggcctgcg cccggagatc gaccgtaccg tcctgcgcaa
     3601 cctcctgctc gcctcgttgc cgcccggtgc cgtggcctgg ggccacaagc tcaccgccgc
     3661 cgagcgggcg gagggtggcg ggttccacct ggccttcagc gatggccgca ccacgtgggc
     3721 cgagctgctc gtgggcgccg acggcgcctg gtcgagggtc cggccgctcc tgtccgccgc
     3781 cgtgcccgag tacgtgggga tcacctacct cgagttcgag ctcacggacg tcgcgacccg
     3841 gcatcccgag ctggcgcggc tggtcgggac cggcagcctc ttcgccctgt cggacaacaa
     3901 gaacatcggc ggccgcggcg gcggccacct gcatctcgga gccggcctgc gggtcccgga
     3961 ggactgggtg cgctcctccg gcgtcgactg ggccgatccg gacgccgccc gggccgccct
     4021 gctggccgag ttcgccgact ggtccgaggg actgaaggac ctgatccgcc actgtgacga
     4081 ccggatcacc ccccggctga tccacgccct gccgatcggg cactcctggt cccgcgggcg
     4141 gggcgtgacg ctgctcggcg acgccgccca cctgatgtca ccgttcgccg gggagggcgc
     4201 caacgccgcg atgctcgacg cggccgtcct ggccgggttc ctgctggagc acgaggatga
     4261 cgtcgagacc gcgctggccc gctacgaggc cgacatgttc ccgcgcgccg ccgaggcggc
     4321 ccggcagtcc gccttcgggc tcgacctgat cttc
//
LOCUS       DASVOR010000430         9695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_179486,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000430 DASVOR010000000
VERSION     DASVOR010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9695
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179486"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF516_08545"
     CDS             complement(<1..217)
                     /locus_tag="VF516_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107766.1"
                     /translation="MTRARTSGRRRAGGFTTRMPRIERFGLGALGIAGVALGFELIPR
                     AGLVSPHYLAPTSAMLGALGREAATAAF"
     gene            complement(214..1233)
                     /locus_tag="VF516_08550"
     CDS             complement(214..1233)
                     /locus_tag="VF516_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8107767.1"
                     /translation="MCRHTVPRICALTALCLQLGLLASCSRSKSDGAGAGSSAATGAP
                     KDSPAPAGAGPIKLTVAAIPIVDVAPLYLGKQKGLFGAEGLELDIQNTQGGAQSVPCV
                     VSGQCQFGFANVISLLLGHAKGLPLQVVAAGNFSTGKAEDFGAIIVPEGSPVKTMKDL
                     EGKTLSVNQINNIGGVTVRAAMRKAGGDPDKIKFIEVPFPEMPAALGQKRVDAAWVVE
                     PFLTVARGQGATVLDWNFADTAPTLMIAAYFTSRDYAQGHADVVQRFTAAMNKSLSYA
                     AEHPDEARAVLLTYTKIDKAVTEKLNLPLWSPQIHRDSIHLLADLVVQDKLVPQKPDV
                     DALLR"
     gene            1528..1908
                     /locus_tag="VF516_08555"
     CDS             1528..1908
                     /locus_tag="VF516_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107768.1"
                     /translation="MTPNLYQLSGHHLHVTYSPTGIDGKPHMTYQDTHQSKSFKGDEI
                     RTVECDLGTLVSVTLRMTVDIGSTTLSVLIPRMRISPASVAAVHTECITTVHNTSFAP
                     LGIQGQLDTYTVTSLRGTAQAVAY"
     gene            2304..4049
                     /locus_tag="VF516_08560"
     CDS             2304..4049
                     /locus_tag="VF516_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107769.1"
                     /translation="MHVMARPQVFRITPWLGGFFSAGIVAATAIAAGLILTLDLVVIS
                     LLMISRGEGLAASVALGALALTPWVVVFILSFRATRSSLRGMLWRIGEWLWISVVATP
                     IAGIALGLTTGIWLSPQNGLSALSPEAQLGVIPLTAIVVRAICKRILSSPRARIGVFR
                     RFTPRASRAARRTLAPVLGAYGGVALASDADFQIIMLDSFLGVDPDRFGDAEAQPQFD
                     DATWQTEVTALIADIQIAVFDVTEWSNSLEWELHECARHLPAHCILFVTEAGGARAEW
                     FGSAAKLPTTHADVLAAALRRPLWYRVLGFRLQLFSWMRRVAMQPPLAQAPRPRLRGA
                     VRWALALFLAVDGSLLAADCAVRLARQQSCSPGDIMLRSDVLGREHCVRAATNGLFPL
                     DERPTAGPARLFDSRGALLAAGRIGPAAGRGPDDGAVRQDEWTFHYPDGRLRAKGSFI
                     DNQRFGRWIFFDEQGKVTARGHYVAGQMEGSWTFLGSAGQPLQEGSFIHGRREDEWSL
                     YCVSGALRGRGKFRDGQVVFGTWTLYSSCGEGYAEPQSPIVDGFDRLASALIEPVVER
                     EPYRLDDEFGRLPEW"
     gene            complement(4106..5110)
                     /locus_tag="VF516_08565"
     CDS             complement(4106..5110)
                     /locus_tag="VF516_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8107770.1"
                     /translation="MKIDIFNHLYPKRFFDQFIADGAAGKDMGKRVANIPTIVDLDAR
                     FRAMDEFGDFRQVLTLPSPPLEALAGPDRSPAMARAANDQLAELVARHPDRFIAFVAS
                     LPLNNPDESLKEMERAVTQLGARGIQIYSNVAGKPLDEPEFEPLFAEAARRDLPIWMH
                     PARKADFPDYKTEDKSKYEIWWTFGWPYETSVAMSRMVFSGMLDRYPNLKIITHHMGG
                     MIPYFEGRVGHGWDQLGTRTSDVDYKALLRSMKKRPIDYFRSFYADTALFGARAATSC
                     GLDFFGADHVVFASDMPFEPAPGMYARETIQVIESLGLSEADKHKIYCGNAQRLLKLT
                     "
     gene            complement(5107..6450)
                     /locus_tag="VF516_08570"
     CDS             complement(5107..6450)
                     /locus_tag="VF516_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8107771.1"
                     /translation="MSEAPPAFMEPAGTLDVRRLIDERPASRFQVGVAVLCAAIVFLD
                     GFDALIMSYVAPALRAQFHFDKLALGPVLSFGLIGMLVGALVFGPLADRIGRRPVLLL
                     CPLIFGAGSLLAGNASSVTELLVFRLVTGFGMGGAMPNAIALTAEYMPKRIRARAVTT
                     MFCGFSLGSAAVGWVAAAVIPTLGWPWVFRIGGILPLAIAAAAFAWLPESVRFLVRRN
                     SGDPRARRYVARIAPEMQGAAQTATLTVEEEPRGFPVVQLFTGGRHWVTVLLWVMFFA
                     NLLDLYFVNSWMPLIMKDVGMPDGRAIVITTLFQVGGSIGAYLIGMLFDRNLSFRVLA
                     LGYLTAAVFVFLIGESGTSTVWLVLTVFMSGFGVVGSQNGANAMAAEVYPTAARSTGI
                     GWALGVGRIGSILGPNLGVMMVGATPRLFLYAAVPLMIAATAALVASVVHKESPT"
     gene            6634..8364
                     /locus_tag="VF516_08575"
     CDS             6634..8364
                     /locus_tag="VF516_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEX8107772.1"
                     /translation="MPNLLAMSFEGELAPSFTLHCLEPGRTLPDGWGLGYYPGGEPSA
                     AILKEPAPPQASIRSQLALAWEQVASSMFVLHIRHATWGALSDANTQPFARTWGRRDW
                     LIAHAGSLDRKPAEVPGPFEPVGSTDTETMFCGLLNRFAERGWRNLGQVDLGVLVDWL
                     AELNEVGSLSMCFTDGRDLVVHADRRGVPLWLGTLAPPYDKIAFGDDDLDVDLTRRGA
                     KSRKGAIVCSEQLAPRGADAERALHWRQLEPGRVLVIREGAVIEERSSGGALDPLKPR
                     LRPARTVSRIFDVVHRTTYKYTRPVERSAHVLRLTPIHDRMQTVHSHELTFSVPHQRR
                     DYEDVFGNRVRRVEIDTPFSELVIEARSVVEVLDVDPLAAEPLHVSSTIPLVWMPWQR
                     QVLDPYLLPPELPETELAELAEYAMSFVKRNDSDLLATLLDINDTLHREYAYQQGITN
                     NHTTAFDVYVNRRGVCQDFTNLFICLARLLGVPARYVCGYVYTGPKSAELLPHQRQGE
                     ASHAWVQLYLPQLGWKGFDPTNGILTQTDHVRTAVGRNRLDATPTSGTIFVGGAGETL
                     AVEVRVEPHG"
     gene            8444..8731
                     /locus_tag="VF516_08580"
     CDS             8444..8731
                     /locus_tag="VF516_08580"
                     /inference="COORDINATES: protein motif:HMM:NF015640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HEX8107773.1"
                     /translation="MPTRNISLTREQDAFVEEMVDSGDYQNASEAIRDALRVLQQRRR
                     EDKLKLQALRAQLKLGVDALRRGDFDEVDEANLHLYLKSLTGAGRKHRQAP"
     gene            8741..9118
                     /locus_tag="VF516_08585"
     CDS             8741..9118
                     /locus_tag="VF516_08585"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8107774.1"
                     /translation="MAGFRISGPARADLEQILAVSLERWGEDGQARYRGLLEAALRAI
                     ASAPEGPVTRDRSLLGAGLRSFHLRHARRAHGVHEPVHIIYYRPMRSASIEVVRVLHE
                     RMDPTAHLDAATPRRTPGKRSQS"
BASE COUNT         1524 a         3486 c         3188 g         1497 t
ORIGIN      
        1 agaacgccgc ggttgccgcc tcgcggccga gcgcgccgag catcgcgctg gtcggcgcca
       61 ggtagtgcgg cgacaccagc ccggcgcgcg ggatgagctc gaaccccagg gccacgccgg
      121 cgatgccgag cgccccgagc ccgaaccgct cgatccgcgg catgcgggtc gtgaacccac
      181 cggcccgccg gcgcccggac gtccgcgccc gggtcatcgc agcagcgcgt cgacgtccgg
      241 cttctggggc accagcttgt cctggaccac caggtcggcg agcaggtgga tcgagtcgcg
      301 gtggatctgc ggcgaccaca gcggcaggtt gagcttctcg gtgaccgcct tgtcgatctt
      361 ggtgtacgtg agcaggaccg cccgcgcctc gtcggggtgc tcggccgcgt agctcagcga
      421 cttgttcatc gccgcggtga accgctggac cacgtcggcg tggccctgcg cgtagtcccg
      481 gctcgtgaag tacgccgcga tcatcagcgt cggcgccgtg tcggcgaagt tccagtcgag
      541 cacggtcgcg ccctggccgc gcgccacggt caggaacggc tcgaccaccc acgccgcgtc
      601 gacccgcttc tggccgagcg ccgccggcat ctcggggaac ggcacctcga tgaacttgat
      661 cttgtccgga tcgccgccgg ccttgcgcat cgccgcgcgc accgtgaccc cgccgatgtt
      721 gttgatctgg ttcaccgaca gcgtcttgcc ctcgaggtcc ttcatcgtct tcaccggcga
      781 gccctccggg acgatgatcg cgccgaagtc ctcggccttg ccggtcgaga agttccccgc
      841 cgcgacgacc tggagcggca gccccttggc gtggccgagc agcagcgaga tcacgttggc
      901 gaagccgaac tgacactgcc cgctcaccac gcagggcacc gactgcgccc cgccctgcgt
      961 gttctggatg tcgagctcca ggccctcggc gccgaacagc cccttctgct tgccgaggta
     1021 cagcggcgcg acatcgacga tcgggatcgc ggcgaccgtg agcttgatcg gccccgcccc
     1081 ggccggcgcc ggcgagtcct tcggcgcgcc cgtcgcggcg ctcgatccgg cgccggcgcc
     1141 gtcggacttc gaccgcgagc agctggcgag cagcccgagt tgcaagcaca gcgcggtgag
     1201 cgcgcagatc cgtggcacag tgtgccggca catggggtcc tcctggaacg tcgtcggaga
     1261 cgacgccaca gcctaaaaaa ttccacccgg gggcgcaagc cggggcgcgg cgcggccttg
     1321 ggaagctgtg tttggcaacc gcacggctgc gccgcgcgcg gcacgcttgt tgcgatgcga
     1381 ggcggtggca gtcagcgatc ctccgggacc ggcgcctgac ggtccatctc gtggagcttt
     1441 tcaacaggcg cgcgccggcg cctcagccat tcaggaacag tcatgcctca gtcttcgcct
     1501 tcgtctcagt ctccgggcac gccaaccgtg acaccgaatc tttaccagct cagcggtcac
     1561 cacctgcacg tgacctactc gccgacgggc atcgacggca agccgcacat gacctatcag
     1621 gacacccatc agagcaagtc gttcaagggc gacgagatcc gcacggtcga gtgcgatctc
     1681 gggaccctgg tcagcgtcac gctccggatg accgtcgaca tcggctcgac gacgctcagc
     1741 gtgctcatcc cgcgcatgcg gatctcgccg gcctcggtcg ccgcggtcca caccgagtgc
     1801 atcacgaccg tgcacaacac gtcgttcgcg ccgctgggga tccagggcca gctcgacacc
     1861 tataccgtca ccagcctccg cggcaccgcg caggccgtgg cgtactagcc gcatccttca
     1921 ccccgcgtgg cgtggcagcc acgtggcagc cggagtggtg gcggtctagt ctgtgtcgag
     1981 ccagcttcac atgccaggcc gaagtcggac gcgtggaccc cggccatcgt agctacggcc
     2041 agcgcagcga gcgcggcctc ggctggcgca ccggaccgtc gtggacggga gagggacctg
     2101 gcagcggtgg tccgcacagc gcatggacga tgtcaaccac tcgctggtcc atggcatcgc
     2161 gccgaagaac gtcgaagttc ctgtgcagat gcgcttggat actccccctc ccctggcagg
     2221 agtgcattcg agctgcggcg tactacgctg cgatcaaggg gatggcctcg actggtcgag
     2281 acgtggctgg agatgagcct gtcgtgcatg tgatggcgcg ccctcaggtc ttccggatca
     2341 caccctggtt gggcgggttc ttctcggcgg ggatcgtcgc cgcgaccgcg atcgcggcag
     2401 gcctgatctt gaccctggac ctcgtcgtga tctcgctgct catgatcagc cgaggcgagg
     2461 gccttgcggc gtcggtggcg ctcggtgcgc tcgctctgac cccctgggtg gttgtgttca
     2521 tcttgtcgtt ccgcgccacg cgctcgagcc tccgcgggat gctctggcgg atcggcgaat
     2581 ggctgtggat ctccgtggtg gcaacgccaa tcgccggtat cgccctgggg ctcacaaccg
     2641 gcatatggct aagcccgcag aatggcttgt ccgcactatc accggaggcc caactcgggg
     2701 tgattcccct caccgccatc gtcgtgagag ccatctgcaa acggatcctc tcgtccccac
     2761 gtgcgcgaat cggcgtgttt cgccggttca cccccagggc ctcgcgcgcg gcccggcgca
     2821 cgctggcacc ggtgctgggc gcgtacggcg gcgtggccct ggcgagcgac gccgacttcc
     2881 agatcatcat gctcgacagc tttctggggg tcgatcccga tcgtttcggc gacgccgagg
     2941 cccagccgca gttcgacgac gcgacctggc agaccgaggt caccgcgctg atcgccgaca
     3001 tccagatcgc cgtgttcgac gttaccgagt ggtcgaacag ccttgaatgg gagctccacg
     3061 agtgcgcgcg ccatctgcca gcgcattgca tcctattcgt caccgaggcc ggtggcgcgc
     3121 gcgctgagtg gtttgggtcg gccgcaaagc tccctacgac gcacgcggac gtgctcgctg
     3181 ccgcgctgcg tcggccgctg tggtaccgtg tcctcgggtt ccggctccag ctgttctcgt
     3241 ggatgcgccg cgtggccatg cagccgccgc tggcccaggc gccgaggccg cgcttgcgcg
     3301 gagctgtccg atgggcgctc gccctgttct tggcagtcga cggttctctc ctcgcggcgg
     3361 actgcgcggt gcgcttagcg cggcagcaga gctgctctcc cggtgacatc atgctgcgct
     3421 ccgatgtgct cgggcgcgaa cactgcgtga gggctgccac caacggcctg ttcccgctgg
     3481 atgagcgtcc gacggcaggt cctgcccgcc tgtttgacag ccgcggcgcg ctgctcgcgg
     3541 ccggacgcat cggccccgct gctggtcgcg gccccgacga tggggcagtc cggcaagacg
     3601 agtggacgtt ccattacccc gacggccgcc tcagggcgaa ggggagcttc atcgataatc
     3661 agcggttcgg tcgatggatc ttcttcgacg agcaaggcaa agtgacggcc agaggccact
     3721 acgtggcggg gcagatggag gggagctgga cgttccttgg atctgcgggg cagcccctgc
     3781 aagagggtag tttcatccat ggtcgacgcg aggatgaatg gtcactctac tgcgtgagcg
     3841 gagcgctgcg cggtcggggc aagttccgcg acgggcaggt cgtgttcgga acatggacgc
     3901 tctactcgtc ttgcggcgaa ggatacgccg agccacagag cccgatcgtg gatgggtttg
     3961 atcggctggc gagcgctctg atcgagccgg tggtcgagcg cgagccctat cgcctcgatg
     4021 acgagtttgg tcgcctgccg gagtggtagc ggccggaacg agccgcccag ggcagtcttc
     4081 tgctcgcgcg acggatgagt caggatcagg tcagcttgag caggcgctgc gcgttcccgc
     4141 agtagatctt gtgcttgtcg gcctccgaga gccccaggct ctcgatcacc tggatcgtct
     4201 cgcgcgcgta catcccgggc gccggctcga acggcatgtc ggacgcgaac acgacgtggt
     4261 cggcgccgaa gaagtccagg ccacagctcg tcgccgcgcg cgccccgaac agcgcggtgt
     4321 cggcgtagaa gctgcggaag taatcgatcg gccgcttctt catcgagcgc agcagcgcct
     4381 tgtagtcgac gtccgaggtc cgcgtgccca gctggtccca gccgtggccg acccggccct
     4441 cgaagtacgg gatcatcccg cccatgtggt gcgtgatgat cttcagattc gggtaccggt
     4501 cgagcatccc gctgaacacc atgcgtgaca tcgccacgct ggtctcgtac ggccagccga
     4561 acgtccacca gatctcgtac ttcgacttgt cctcggtctt gtagtccggg aagtcggcct
     4621 tgcgcgccgg gtgcatccag atcggcagat cgcgccgcgc ggcctcggcg aacagcggct
     4681 cgaactcggg ctcgtcgagc ggcttgcccg cgacgttgga gtagatctgg atcccgcggg
     4741 ccccgagctg ggtcaccgcg cgctccatct ccttgagtga ctcgtcgggg ttgttgagcg
     4801 gcagcgacgc cacgaacgcg atgaagcggt cggggtgcct ggcgaccagc tcggcgagct
     4861 ggtcgttggc cgcgcgcgcc atcgccggtg agcgatccgg cccggccagc gcctcgagcg
     4921 gcggcgacgg cagggtcagc acctggcgga agtcgccgaa ctcgtccatc gcgcggaacc
     4981 gcgcgtcgag gtcgacgatc gtcgggatgt tcgcgacccg cttgcccatg tccttgccgg
     5041 ccgcgccgtc ggcgatgaac tggtcgaaga accgcttggg atagaggtga ttgaagatat
     5101 cgatcttcat gtggggcttt ccttgtgcac gacggaggcg accagcgccg ccgtcgcggc
     5161 gatcatgagc ggcaccgcgg catacaggaa cagccgcggc gtcgcgccga ccatcatgac
     5221 gccgaggttc ggcccgagga tcgagccgat ccggccgacg ccaagcgccc agccgatccc
     5281 ggtcgaccgg gccgcggtcg gatagacctc ggcggccatc gcgttggcgc cgttctgcga
     5341 tccgacgacg ccgaaccccg acatgaacac cgtcaggacc agccacaccg tcgaggtgcc
     5401 cgactcgccg atcaggaaca cgaagaccgc cgcggtcagg tagcccagcg ccagcacgcg
     5461 aaacgacagg ttgcgatcga acagcatgcc gatcaggtac gcgccgatcg agccgccgac
     5521 ctggaacagc gtggtgatga cgatcgcgcg gccatcgggc atcccgacgt ccttcatgat
     5581 cagcggcatc cagctgttga cgaagtacag gtcgagcagg ttcgcgaaga acatcaccca
     5641 cagcagcacg gtgacccagt gccgcccgcc ggtgaacagc tgcaccaccg ggaacccgcg
     5701 cggctcctcc tcgacggtca gcgtcgcggt ctgcgccgcg ccttgcatct ccggtgcgat
     5761 ccgcgccacg tagcgacggg cgcgcggatc accggagttg cggcgcacca ggaagcgcac
     5821 cgactccggc aaccacgcga acgccgccgc ggcgatcgcg agcggcagga tcccgccgat
     5881 ccggaacacc cacggccagc ccagagtggg gatcaccgcc gccgcgaccc agccgaccgc
     5941 ggccgagccg agcgagaacc cgcagaacat cgtcgtcacc gccctcgccc ggatccgctt
     6001 gggcatgtac tcggcggtca gcgcgatcgc gttgggcatc gccccgccca tcccgaaccc
     6061 ggtcaccagc cggaacacca gcagctcggt caccgagctc gcgttgccgg cgagcagcga
     6121 gcccgcgccg aagatcagcg ggcagagcag cagcaccggg cggcggccga tccgatcggc
     6181 gagcgggccg aacaccagcg caccgacgag catgccgatc agcccgaacg acagcaccgg
     6241 cccgagcgcc agcttgtcga agtggaactg cgcgcgcagc gccggcgcga cgtagctcat
     6301 gatcagggcg tcgaacccgt cgaggaacac gatcgccgcg cacagcaccg cgacgccgac
     6361 ctggaagcgg ctcgccggcc gctcgtcgat caaccggcgg acgtcgagcg ttcctgccgg
     6421 ctccatgaac gcgggtggag cctccgacat ggcgcggcca gcgtagacaa gccacggtgg
     6481 caacacaagc gcgattcagc cgcgccaccg gcacacagtt ttgcgatcgc gcgcgacgcg
     6541 cacaccgcgc gccgatgccg cgcatgcatc cgtcgcgatc tctcgacgcg ccgcgccgtg
     6601 ccccgttccg cgagactcgg ctagagtcgc ccggtgccca acctgctcgc gatgtcgttc
     6661 gagggcgagc tcgcgccgtc gttcacgctg cactgcctgg agcccggacg tacgctcccc
     6721 gacggctggg gcctcggcta ctaccccggc ggcgagccgt ccgccgcgat cctcaaggag
     6781 cccgccccgc cccaggcctc gatccgcagc cagctcgccc tggcctggga gcaggtcgcg
     6841 tcgagcatgt tcgtgctcca catccgccac gccacctggg gcgccctgtc ggacgccaac
     6901 acccagccgt tcgcccggac ctggggccgc cgcgactggc tgatcgccca cgccggcagc
     6961 ctcgaccgca agcccgccga ggtccccggc ccgttcgagc cggtcggctc caccgacacc
     7021 gagaccatgt tctgcggcct gctcaaccgc ttcgccgagc gcggctggcg caacctcggc
     7081 caggtcgacc tcggcgtgct ggtcgactgg ctcgccgagc tcaacgaggt cggctcgctg
     7141 tcgatgtgct tcaccgacgg ccgcgacctg gtcgtccacg ccgaccgccg cggcgtcccg
     7201 ctgtggctcg gcaccctcgc cccaccctac gacaagatcg cgttcggcga cgacgacctc
     7261 gacgtcgatc tcacccggcg cggcgccaag agccgcaagg gcgccatcgt gtgcagcgag
     7321 caactcgccc cgcgcggcgc cgacgccgag cgcgccctgc actggcgcca gctcgagccc
     7381 ggccgcgtcc tggtcatccg cgagggcgcc gtgatcgagg agcgctccag cggcggcgcg
     7441 ctcgacccgc tcaagccccg cctgcgcccg gcccgcaccg tgtcgcggat cttcgacgtc
     7501 gtccaccgca ccacctacaa gtacacccgg ccggtcgagc gctccgccca cgtgctccgg
     7561 ctcaccccga tccacgatcg catgcagacc gtgcactccc acgagctcac gttctccgtg
     7621 ccgcaccagc gccgcgacta cgaggacgtg ttcggcaacc gcgtccgccg ggtcgagatc
     7681 gacacgccgt tctccgagct ggtcatcgag gcgcgcagcg tcgtcgaggt gctcgacgtc
     7741 gacccgctcg ccgccgagcc gctccacgtc agctccacca tcccgctggt ctggatgccc
     7801 tggcagcgcc aggtcctcga tccctacctg ctcccccccg agctccccga gaccgagctc
     7861 gccgagctcg ccgagtacgc gatgagcttc gtcaagcgca acgactccga cctgctcgcc
     7921 accctgctcg acatcaacga caccctccac cgcgagtacg cctatcagca gggcatcacc
     7981 aacaaccaca ccaccgcgtt cgacgtctac gtcaaccgcc gcggcgtgtg ccaggacttc
     8041 accaacctgt tcatctgcct ggcccgcctg ctcggcgtcc cggcgcgcta cgtctgcggc
     8101 tacgtctaca ccggccccaa gtcggccgag ctcctgcccc accagcgcca gggcgaggcc
     8161 tcccacgcct gggtccagct ctacctgccc cagctcggct ggaagggctt cgaccccacc
     8221 aacggcatcc tgacccagac cgaccacgtc cgcaccgcgg tcggccgcaa ccgcctcgac
     8281 gccaccccga ccagcggcac gatcttcgtc ggcggcgccg gcgagaccct cgccgtcgag
     8341 gtccgcgtcg agccgcacgg gtagcgggga gcgctgcgag cccacggccg atcggggcca
     8401 tggctcggtt ggcaaacttt gtcatcctgg taggatcgac cagatgccga cgcgcaacat
     8461 cagcctcacc cgagagcaag atgccttcgt cgaggagatg gtcgactcgg gagactatca
     8521 gaatgcgagc gaggcgatcc gcgacgcgct gcgtgtgctg cagcagcgtc ggcgcgagga
     8581 caagctcaag ctccaggcgc tgcgcgcgca actcaagcta ggcgtcgatg cccttcgccg
     8641 tggtgacttc gatgaggtcg acgaagcgaa cctccatctc tacctgaagt cgttgaccgg
     8701 agccgggcgc aagcatcgcc aggcgccctg atccgcggcc atggctggct ttcgcatctc
     8761 cggccctgcg cgagccgatc tggagcagat cctggcggtc agcctcgaac gatggggaga
     8821 ggatggccag gcgcgctacc gcgggttgct ggaggcagca cttcgtgcga tcgcttcggc
     8881 cccagagggg cccgtcacac gagatcgcag cctgctcggg gccgggctac gcagcttcca
     8941 tctgcggcat gcacgccgtg ctcacggtgt ccacgaaccg gtgcacatca tctattaccg
     9001 gccgatgcga tcggcgtcga tcgaggtggt acgcgttcta catgagcgga tggatccgac
     9061 ggcgcacctc gacgccgcga cgccgcgcag aacgcccggc aagcggtctc agtcgtagcc
     9121 actgcacaac ccgattacat ggtattcctt gtgtgacacc gcttgccatt cctcgaacga
     9181 tcgctgcccg gaagatcgtc gatgcatccc acggcctggg cgcgacgctg cgagatcctc
     9241 gtagtctcgc ccgtcgctgc acggcccgac gatgcgcttc cacgatggct gcaaatccgc
     9301 tgtggtcata cggcgtcgcc gctcgccgaa ccgccagaac atggtgagtc atgaatcgcc
     9361 accagaacga ggcagcgtcg gcgatcaagc gatcactcgg tggattccga tccttgtgaa
     9421 catgcctatt ccgtgcatga tggcggcgtg cgcacgagcg ctcggactcc atccacggcg
     9481 cgggtgtggt gcggccggtt ggccgcggcc gcggccgcgg agcgctgccc gtgaggcccg
     9541 gagagcagct cgacacccgg ttcgagatcg agcaacggat cggcagcggc ggcatgggca
     9601 tggtgttccg tgcgcgcgat ccgatctcgg gggagacggt ggccgtcaag gtcatctccg
     9661 acgagcgaag ccagctcacc gagcgcttcg cgcgc
//
LOCUS       DASVOR010000431         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_150117,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000431 DASVOR010000000
VERSION     DASVOR010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150117"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VF516_08590"
     CDS             <1..470
                     /locus_tag="VF516_08590"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8107775.1"
                     /translation="PRADVLLCHGTPRSDVEPMLETVELDGPRPATHAEVEARAAGCV
                     APAARLIACGHTHVPRLVRTAGGCVIVNPGSVGLPAYEADHPMAPPEQPAVYYVDNGS
                     PHASYAIAEDTAPGWDVSFHRVAYDWESAAACAERNGRPEWAHALRTGFALRA"
     gene            complement(499..1275)
                     /locus_tag="VF516_08595"
     CDS             complement(499..1275)
                     /locus_tag="VF516_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HEX8107776.1"
                     /translation="MDQLQQIVHRTTEVIGDDLVGAYLHGSSVLGGLRPASDLDILAV
                     TRRSLDEGQRRALVAGLLPISGAAVGARPVELTVVIQADVRPWRYPPIGDFLYGEWLR
                     ADYQAGVVPAPGPMPGLALDIAVALAGDHPLVGPRSAHALDPVPPEHLVRASVEGIPG
                     LLESLPGDTRNVLLTFARIWTTLATGTVVSKDVAADWVLARLAPEHRPVLEFARRLYL
                     ATTYAEETWSDEMKAQVGPHVDEVLAQIHRLQAEIVPAVR"
     gene            complement(1355..1486)
                     /locus_tag="VF516_08600"
     CDS             complement(1355..1486)
                     /locus_tag="VF516_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107777.1"
                     /translation="MRNADRIVVIEDGRIIEHGSHDQLLAAGVRYAAMFTAQAAGYR"
BASE COUNT          235 a          563 c          618 g          230 t
ORIGIN      
        1 ccccgcgcgc cgacgtcctg ctgtgccatg gtacgccgcg atcggatgtc gagccgatgc
       61 tggagacggt cgagctcgac ggcccgcggc ccgccacgca cgccgaggtc gaggcccgcg
      121 ccgcggggtg cgtggctccg gcggctcgcc tgatcgcatg cggccacacc cacgtcccgc
      181 ggctggtccg gaccgccggc ggctgcgtga tcgtcaaccc gggcagcgtc gggcttccgg
      241 cctacgaggc cgaccacccg atggcgccgc ccgagcagcc ggcggtgtac tacgtcgaca
      301 acggcagccc gcacgcctcg tacgcgatcg ccgaggacac cgccccgggc tgggacgtca
      361 gcttccaccg cgtcgcctac gactgggagt ccgccgcggc gtgcgccgag cgcaacggcc
      421 ggcccgagtg ggcccacgcg ctgcgcaccg gcttcgcgct gcgcgcgtga ccgtcgcgtg
      481 ccacgcggaa ccccccggtc accggaccgc agggacgatc tcggcctgca gccggtggat
      541 ctgggcgaga acttcgtcga catggggccc gacctgggcc ttcatctcat cgctccaggt
      601 ctcctccgcg taggtcgtgg cgaggtagag tcgccgggcg aattcgagca cgggccggtg
      661 ctcgggcgcg aggcgggcga gcacccagtc cgcggcgacg tccttggaaa cgaccgtgcc
      721 ggtggcgagc gttgtccaga tccgggcgaa ggtcagcagc acgttgcggg tgtcgccggg
      781 caggctctcg agcagcccgg ggatgccctc cacgctggct cggaccaggt gctccggggg
      841 cacggggtcg agtgcgtgtg ccgatcgggg accgaccagc ggatggtccc cggccagagc
      901 caccgcgatg tcgagcgcca ggcccggcat cggtccgggt gcagggacga cgccggcctg
      961 gtagtcggca cgcagccact cgccgtagag gaagtcgccg atcggcgggt accgccacgg
     1021 gcgcacatcg gcctggatga cgacggtgag ctccaccggg cgagcaccca ccgcggcacc
     1081 cgagatcggc aggaggcctg cgaccagggc gcgtcgctga ccctcgtcca gggatcgccg
     1141 ggtgacggcg aggatgtcca gatcgctggc tggtcgcagc ccgccgagca cggaggagcc
     1201 gtgcaggtac gcgccgacga gatcgtcccc gatcacttcg gtggtccggt gtacgatctg
     1261 ctggagttgg tccatcgcgc ggtccttgac gggtggttgg cgcgtcgaat cgacttgtac
     1321 cgcggcgttc atgcgctatc gaggtcgagt ccgctcagcg gtagccggcg gcctgggcgg
     1381 tgaacatcgc ggcgtagcgg acgccggcgg cgagcagctg gtcgtgcgag ccgtgctcga
     1441 tgatccggcc gtcctcgatc acgacgatgc ggtcggcgtt gcgcacggtc gagaaccggt
     1501 gcgagaccag gctggacgaa gtcctggaac agcgtgccga ggtgggcgta ccagtgcacg
     1561 ggatcgagct cgcgcaggtc gacgccgttg atcaggatct ggccgctggt gacgtcgtag
     1621 aaccggcaca gcagcttgat gagggt
//
LOCUS       DASVOR010000432         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_500951,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000432 DASVOR010000000
VERSION     DASVOR010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500951"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..774
                     /locus_tag="VF516_08605"
     CDS             118..774
                     /locus_tag="VF516_08605"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8107778.1"
                     /translation="MTQQLRDELIATARRMSELGLAPGTTGNVSARTARGFVVTPSGM
                     AYDQLRGDDAVAIDLDGTIRPGQRAPSSEWRLHRDILAARPDVGAIVHTHSLYCTSVS
                     CLRRPIPAIHYMVIHAGGDEIPCADYATFGTAELAASVVRALGRGHACLMANHGMVAL
                     GDSLSAALRLAADVETLAAQYWHAAQLGQPHVLPSDELDRVRAQFAGYGQHRRGRRDG
                     "
     gene            complement(999..1625)
                     /locus_tag="VF516_08610"
     CDS             complement(999..1625)
                     /locus_tag="VF516_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107779.1"
                     /translation="MDKAAARAAVDQVGRIAAAMSAMNSADVAAGVEAMMIAHQTVVA
                     PNDDLGTPFGLLFPYLKFAAPNRGTAVCTAISCSFTGYGFGGGYTVLGITGTVTRSGD
                     SIAFDIRYDLTEHLASVAWTVDGTLSVTQERIEGRVHSHATGSRDATGLRWDMGVDYR
                     AIMLDAQGCPIGGALYAVTSYDIPRDHAGGGPSSVEVEGTVAFGPACQ"
     gene            complement(1853..>2272)
                     /locus_tag="VF516_08615"
     CDS             complement(1853..>2272)
                     /locus_tag="VF516_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107780.1"
                     /translation="EKREWCRAKLRELREQNPHVTWEDVEAEWDKEHPRPPTRIEVIH
                     AELSALIPHPELHGGKKPKNVWAQLEDAGIGFLEFVEKHALEHEEGNLFSYLVRVMNF
                     ARKLYEASQLSEFEDMAERVQKLLARVDIRLIDDRKW"
BASE COUNT          326 a          868 c          730 g          348 t
ORIGIN      
        1 cgtcaccccg gcccgcctgg tcaccgcgct gatcaccgag cgcggcacct gcgcggcgtc
       61 gcccgacggc ctgcgcgcgc tgttccccga gcaggcaccg ggcggagccg atcgcgcatg
      121 acccagcagc tacgcgacga gctgatcgcc accgcgcgcc ggatgagcga gctcggcctc
      181 gcccccggca ccaccggcaa cgtcagcgcc cgcaccgcgc gcggcttcgt ggtcaccccg
      241 tcgggcatgg cctacgacca gctgcgcggc gacgacgccg tcgcgatcga tctcgacggc
      301 acgatccgcc ccggccagcg cgcgccgtcg tccgagtggc gcctgcaccg cgacatcctc
      361 gccgcgcgcc ccgacgtcgg cgcgatcgtc cacacgcatt cgctgtactg cacctcggtc
      421 tcgtgcctgc gccgcccgat cccggcgatc cattacatgg tcatccacgc cggcggcgac
      481 gagatcccgt gcgccgacta cgccaccttc ggcaccgccg agctcgccgc gtccgtggtc
      541 cgcgcgctcg gccgcggcca cgcctgcctg atggccaacc acggcatggt cgcgctcggc
      601 gactcgctgt ccgccgcgct ccggctcgcc gccgacgtcg agaccctcgc tgcccagtac
      661 tggcacgcgg cccagctcgg ccagccccac gtgctgccgt ccgacgagct cgaccgcgtc
      721 cgcgcccagt tcgccggcta cggccagcac cgccgcggcc ggcgagacgg atgaacgacg
      781 aggtgctgcg gtgaccgaga tcctctggga ccctacagga gcaccacatc acggtgcgcc
      841 atggcgtcgc ggccgagaag ttagaaggtg cctggatcga ccggatcgat ctggtcgtcc
      901 gcgacgatga ttcctacgag gaggtagcga tcgagccggg cgatcgcggc gctggtcctt
      961 cgcgacgagc ggtagccgcc cgcggcgcgt ggtcgggatc actggcaggc cggaccgaac
     1021 gcaacggtgc cctcgacctc gacgctcgac ggcccgcccc ccgcatggtc ccgggggata
     1081 tcgtagctcg tgacggcgta cagagcgccg ccgatcggac agccttgggc atcgagcatg
     1141 atcgcgcggt agtcgacccc catgtcccac ctcaggccgg tggcgtcgcg tgagccggtc
     1201 gcgtggctat gcacgcgacc ctcgatgcgt tcctgcgtca cagacagcgt gccatccacg
     1261 gtccatgcca ccgacgcaag gtgctcggtc agatcgtagc ggatgtcaaa cgcgatggaa
     1321 tcgccgctgc gcgtgacggt gccggtgatg ccgaggacgg tgtagcctcc cccgaagccg
     1381 tatcccgtga acgagcagga gatcgcggtg cagaccgccg tacccctgtt cggcgctgcg
     1441 aacttcaggt acgggaacag caggccgaac ggggtgccga ggtcatcgtt cggcgccacc
     1501 acggtctgat gtgcgatcat catcgcctcc acgccggccg ccacatcggc gctgttcatt
     1561 gccgacatcg cggctgcgat ccgaccgacc tggtcgaccg ccgcccgggc cgcggccttg
     1621 tccacgacgc cctcggcagg ctgtgaccca ccgctgcacg ccgccgtggc tactccgatg
     1681 accagagatg tcatgatctc cgtccgcaag ctccacctcc tcgctcccgg caatgtaatc
     1741 acgccaccgc gcacacgctc gtgcgaccac tgcggtcgct tgtcatgcga cgttctgcac
     1801 cgcctgctcg ttgcccccga ggcggcggcg gcgctgcacc caggtgacct cgtcaccact
     1861 tgcggtcgtc gatcagccgg atgtcgacgc gcgccagcag cttctggacc cgctcggcca
     1921 tgtcctcgaa ctcggagagc tgcgacgcct cgtacagctt gcgcgcgaag ttcatcaccc
     1981 gcaccaggta ggagaacagg ttgccctcct cgtgctcgag ggcgtgcttc tcgacgaact
     2041 cgaggaagcc gatgccggca tcctcgagct gcgcccacac gttcttcggc ttcttgccgc
     2101 cgtgcagctc ggggtgcggg atcagcgccg acagctcggc gtggatgacc tcgatccggg
     2161 tcggcggccg cgggtgctcc ttgtcccact cggcctcgac gtcctcccag gtgacgtgcg
     2221 ggttctgctc gcgcagctcg cgcagcttgg cgcggcacca ctcgcgcttc tc
//
LOCUS       DASVOR010000433         4465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_500959,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000433 DASVOR010000000
VERSION     DASVOR010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500959"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1139
                     /locus_tag="VF516_08620"
     CDS             <1..1139
                     /locus_tag="VF516_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107781.1"
                     /translation="LLAELVRDPALRAPLPVPALGPFTLATPELTAVARRVELRPAPD
                     DELEFAIELELDDADRPITTLTAIADVRPELVRTDEGSELVAGFGPDNLRSVDLHLRD
                     QDGRALVDAVARWLPPALRGSVPRAVLEVAARKLAEYLSDRAYDALRATLLRRLGEVT
                     RLRIRLPALPIAATRVTSTPDALTIDLTTDLPVRRGLAAAPPASGPTSGATSGPTSGD
                     VVVQLSGNTAAELTNWSIARGHLPQRYTRGLEPRADGDYRPYFDYLADDRRRPVKIHV
                     FQERNGCSYFQVGLRFEIRLAGDRVEVAVHDRFVEAADAQAAVEVALWLKQLIQGSVD
                     SSYRAAARTELTIGGRRFAARMLGAAAAGDDLTFTLGFATVPGA"
     gene            1367..2044
                     /locus_tag="VF516_08625"
     CDS             1367..2044
                     /locus_tag="VF516_08625"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8107782.1"
                     /translation="MIDLYYWATPNGKKISIALEELALPYQVFPINIGRGEQFKPEFL
                     AISPNNRIPAIVDHAPADGGPSLSVFESGAILLYLADKTGQLIPRDVRGRTTVLEWLM
                     WQMGGIGPMFGQANHFAAYAPEKLPYAIDRYVNETRRLLRVLNTRLEGREFVAGAYSI
                     ADIASFPWVVSARDRLVKDNFDFPNVARWLAAIEARPAVARGMAVRTDARTEMDDEAR
                     KHLFGQR"
     gene            2137..2898
                     /locus_tag="VF516_08630"
     CDS             2137..2898
                     /locus_tag="VF516_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107783.1"
                     /translation="MCEDARSALPHHASRAVQTWTACLAIAHAGIHRPQGDTTMVREP
                     HSGALSASSTTEHGAARRRGTAEDRELDDLRSEVKSAYLGLGEFIRRGILDNKIQGVH
                     NMTTYLSERNTSPVESASLGSALWIGADVAAAALAAATGGATEALVVALTEGSKVVAS
                     TFHRDGNRHSVLDPIKFTNKYYIALTNNWPDSVHHLLKHMTTLADARRIRDQVYKIRD
                     QPAKIIANQQNEILEAWVNAPKARPQGGNTSRSSA"
     gene            complement(3297..3596)
                     /locus_tag="VF516_08635"
     CDS             complement(3297..3596)
                     /locus_tag="VF516_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107784.1"
                     /translation="MTNEQIAMVLDDFDERIRVGGAITGPARPSGPMVLARVSAGTLA
                     SVDVSAVFPEDLYASRSTALDLTQLPLSPSQISDPASGDRRPGFAAHRVHRAPAP"
     gene            3809..4435
                     /locus_tag="VF516_08640"
     CDS             3809..4435
                     /locus_tag="VF516_08640"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8107785.1"
                     /translation="MVIGHVLDATGAPVAGATVTVRASGEHATVDSTGAFTLDVPANT
                     TLTLAANAPSMAPTLLQQFLVPTGTTAQVEIPLITGDHFKTLAAMGMNPSGGVVAVAL
                     KSLSGAGSVAGATVQLTPSLGRVMYAPTGLGMTDPDPAMAAVVPTVVQGNESYAWALG
                     VQPHVSIMQLALRGAAQVEPPYAIDDVIWPGTFTVDAGALTLVTLFTK"
BASE COUNT          689 a         1690 c         1456 g          630 t
ORIGIN      
        1 agctgctcgc cgagctcgtg cgcgacccgg cgctccgcgc gccgctcccg gtgccggcgc
       61 tcggcccgtt cacgctggcg acccccgagc tcaccgccgt cgcgcgccgg gtcgagctcc
      121 gccccgcccc cgacgacgag ctcgagttcg cgatcgagct cgagctcgac gacgccgatc
      181 ggccgatcac cacgctcacc gccatcgccg acgtccggcc ggagctggtg cgcaccgacg
      241 agggcagcga gctggtcgcc ggctttggcc ccgacaacct gcgctccgtc gacctccacc
      301 tccgcgatca ggacggccgc gcgctggtcg atgccgtcgc gcgctggctg cccccggcgc
      361 tgcgtggcag cgtgccacgc gccgtgctcg aggtcgccgc caggaagctc gccgagtacc
      421 tgagcgatcg cgcctacgac gcgctgcgcg cgacgctgct gcgccggctc ggcgaggtca
      481 cccggctgcg gatccggctc cccgcgctgc cgatcgcggc gacccgcgtg acctcgacgc
      541 cggacgcgct caccatcgat ctcaccaccg acctcccggt gcgccgcggc ctcgccgccg
      601 ccccgccggc gtccggcccg acgtccgggg ccacgtccgg ccccacgtcc ggagatgtcg
      661 tcgtccagct gtccgggaac accgccgccg agctcaccaa ctggagcatc gcccgcggcc
      721 acctgccgca gcgctacacc cgcggcctgg agccccgcgc cgacggcgac taccggccgt
      781 acttcgacta cctcgccgac gaccgccgcc ggccggtcaa gatccatgtg ttccaggaac
      841 gcaacggctg ctcgtacttc caggtcggcc tgcggttcga gatccgcctc gccggggatc
      901 gcgtcgaggt cgcggtccac gaccggttcg tcgaggccgc cgacgcacag gccgcggtcg
      961 aggtcgcgct gtggctcaag cagctgatcc agggctcggt cgacagctcg taccgcgccg
     1021 ccgcccgcac cgagctgacg atcggcggcc gccggttcgc cgcgcgcatg ctcggcgccg
     1081 ccgcggccgg tgacgacctc accttcacgc tcggatttgc caccgtgccc ggcgcgtgat
     1141 cgcgtgagcc ggcccatgcg cgcgcctcac gcggcgcgcg gccgggtccg gagtcgacgc
     1201 ttgacaacct gcatcggagc cgccctcctt gaaccgctcg tgtgcggcgt cgtcatcgtc
     1261 gtcccggcga tgggcctcgg gcaccggcgg tcgaacctgc cggcggcccg gatccaccgc
     1321 gactagtcca ttgccggagc ccggtcggcg cggtacgcac ggcgccatga tcgacctcta
     1381 ttactgggcg accccgaacg gcaagaagat cagcatcgcg ctcgaggagc tggcgctgcc
     1441 gtaccaggtg ttcccgatca acatcggccg cggcgagcag ttcaagcccg agttcctggc
     1501 gatcagcccc aacaaccgga tcccggcgat cgtcgaccac gctcccgccg acggcggtcc
     1561 gtcgctctcg gtgttcgagt ccggcgcgat cctgctctac ctcgccgaca agaccggcca
     1621 gctgatcccg cgcgacgtgc gcggccgcac caccgtgctg gagtggctga tgtggcagat
     1681 gggcggcatc ggtccgatgt tcggacaggc caatcacttc gccgcctacg ctcccgagaa
     1741 gctgccgtac gcgatcgacc gctacgtcaa cgagacccgc cggctgctcc gcgtgctcaa
     1801 cacccggctc gagggccgcg agttcgtcgc cggcgcctac tcgatcgccg acatcgcgtc
     1861 gttcccgtgg gtggtctcgg cgcgcgaccg gctggtcaag gacaacttcg acttccccaa
     1921 cgtcgcccgc tggctcgccg cgatcgaggc gcggcccgcg gtcgcccgcg gcatggcggt
     1981 gcggaccgac gcgcggaccg agatggacga cgaggcccgc aagcacctgt tcggtcagcg
     2041 ctgagctgtc tgcgactccc ggacgcaggc ggccggggct gcgctgcggt cccagcttcg
     2101 cccacattgg ccatgtgcag gcgaatcgca tgcacgatgt gcgaggacgc gcggagcgcc
     2161 ctgccgcatc atgcatcacg agcggttcag acatggaccg cctgccttgc tatagcgcac
     2221 gcaggcattc acaggccgca gggggacacc acgatggtca gagagccgca cagcggggca
     2281 ctgtctgcgt cgagcaccac cgagcacggc gcggcccgca ggcggggaac cgcggaggac
     2341 cgggagctcg acgatctcag atccgaagtc aagagcgcgt atctgggtct gggagagttc
     2401 atcaggcgcg gcattctcga caacaagatc cagggcgtgc acaacatgac gacgtacctg
     2461 tccgagcgga atacgtcgcc cgtcgagtcc gcgtctctgg gcagcgcgct gtggatcggc
     2521 gcggacgtcg cggcggctgc gctcgcggcg gcaaccggcg gtgccaccga ggcgctcgtc
     2581 gtggcgctga cggagggcag caaggtcgtg gcatcgacct tccaccggga cggcaatcga
     2641 cactccgtcc tggatcccat caagttcacg aacaagtact acatcgcgct caccaacaac
     2701 tggcccgact ccgttcacca cctgctcaag cacatgacca cgctcgccga tgcccggcgg
     2761 atcagagatc aggtgtacaa gatccgcgac cagccggcga agatcatcgc caaccagcag
     2821 aacgagatcc tcgaggcatg ggtgaacgcg cccaaggctc ggccccaggg cggcaacacc
     2881 tcgagaagct cggcgtgacc cagatccgca gctagcaaag agcggcatcg gccggccgcg
     2941 acgactgcaa cggagcgcgg gcgcgtcgcc gctcgactcg ccggtcgccg cggaatggca
     3001 aacccgagcc accgctgccc cgaggatgga tctcgacagg gcagctccgg agcttgcgcg
     3061 aatcgcgcgc cgcattcgcc atcggcgtcc agtgccggat gcggtcgcag gtgcacaggg
     3121 attgtccggc ggtgacgacg agacactcgg gtcggcaatg gaccgcagcg tgtcgctaca
     3181 ggcgccggca ctcgaattcc tgagtcgccc gtgcgcgcgg acggcgcaat tcacgccgcg
     3241 cgacgccccc tggaccttcg agggaccccc ctggaccttc gaggtctccc ctgcgttcag
     3301 ggcgcaggcg cgcgatgaac gcggtgagcg gcaaacccgg gccgccgatc gccggacgct
     3361 ggatcggaga tctgggacgg tgacaagggc agttgcgtga ggtcgagggc agttgaccgt
     3421 gaggcgtaga ggtcctctgg gaacacggcg ctcacatcga ccgatgcgag cgttcccgca
     3481 ctgactcgcg cgaggaccat cggtccggat ggccgcgccg ggccggtgat cgcgccgccg
     3541 accctgatcc gctcatcgaa gtcgtcgaga accatggcga tctgctcgtt cgtcacgggc
     3601 ggacgggccg ccgatcgaga gtcggcattg ctcgtgcaca gcggcctggg catccaaaac
     3661 ggttcgtaat cagaaacacg ggttcaccat gcgtagcagt catcgagtca tcgggttttc
     3721 agtagttctg gcctctctcg ccggttgcgc ggacaacctg gtgacccaca ctgcccctga
     3781 aacgcctacc accggcacgg accgcgccat ggtcatcggc cacgtcctgg atgccaccgg
     3841 cgctcccgtc gccggcgcga cggtgaccgt ccgcgcctcc ggggagcacg ccaccgtgga
     3901 cagcaccggc gcgttcaccc tcgacgtccc cgccaacacc acgctgaccc tcgccgcgaa
     3961 cgcgcccagc atggcgccca ccctcctcca gcagttcctg gtcccgaccg gcaccaccgc
     4021 ccaggtggag atcccgctga tcaccggcga ccacttcaag accctggccg cgatgggcat
     4081 gaacccctcc ggtggcgtgg tcgccgtcgc cctcaagtcg ctgtcgggcg ccggcagcgt
     4141 cgccggtgcc accgtccagc tcaccccgag cctgggccgc gtgatgtacg cgcccaccgg
     4201 cctgggcatg accgaccccg accccgcgat ggcggccgtc gtgcccaccg tcgtgcaggg
     4261 caacgagtcc tacgcctggg cgctcggtgt ccagccccac gtctccatca tgcagctcgc
     4321 cctccgcggc gcggcccagg tcgagccccc ctacgccatc gacgacgtca tctggcccgg
     4381 caccttcacc gtcgacgccg gcgcgctcac cctcgtcacg ctgttcacca agtgaggtct
     4441 ccgatgaagc ccgtgtacac cctcg
//
LOCUS       DASVOR010000434         4028 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_62500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000434 DASVOR010000000
VERSION     DASVOR010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4028
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VF516_08645"
     CDS             <1..449
                     /locus_tag="VF516_08645"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8107786.1"
                     /translation="SAGGTPVVGSGPCRFTVATTPAGAVVRFDDQPMGPSPITIEGSC
                     DKHKVDVAHARYQSVTRWVTLAADRTQQLDISLPRPIHAVTVTSSPPGAELSIDGHHA
                     GTTPTVVQMAGFATVNLTFTKPGFQTVTRKVYSKQAQDRVSVKLTK"
     gene            complement(461..799)
                     /locus_tag="VF516_08650"
     CDS             complement(461..799)
                     /locus_tag="VF516_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107787.1"
                     /translation="MLAALLVAGAALTAPAMTGCYASGGAYVVDEEPPPPREEVVVSR
                     PGFLWIHGHWHHDGGRWAWRGGYYERERPEQVYVEGRWERRAKGHVWVEGGWRARGNV
                     VVRDHDHDHH"
     gene            1061..2521
                     /gene="guaB"
                     /locus_tag="VF516_08655"
     CDS             1061..2521
                     /gene="guaB"
                     /locus_tag="VF516_08655"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEX8107788.1"
                     /translation="MFEDLREALTFDDVLLVPGYSEVIPRDVDVSTRLTPAIPLRMPL
                     VSSAMDTVTEAATAIRMAREGGIGFIHKNLSIEDQAREVNRVKKAQSGIVVDPVTIAP
                     SRTLEDALALMRHYRISGLPVVDADRRPVGILTNRDVRFEKRLQLPVGEMMTKNPVTV
                     KEGVQIDEAKDLLHKHRIEKLLVVDREGRLKGLITIRDIEQAEAHPTAATDELGRLRV
                     GAAVGVGGDREARIEALLAAGCDVICIDTAHGHSAGVLEAVQKTRKRFGKLQLVAGNV
                     ATGDATRALIECGVNAVKVGVGPGSICTTRIVAGVGVPQLTAIGDAVAAARGSGVAII
                     ADGGIKYSGDVAKALAAGADTVMIGSLFAGTDEAPGEIILYQGRSYKTYRGMGSIGAM
                     KDGSKDRYFQGDVEAPQKLVPEGIEGRVPYKGPLRESLYQLVGGLRASMGYLGCATIA
                     ELHAKARFVKISSAGLRESHVHDVIITKEAPNYRVE"
     gene            2523..>4028
                     /gene="guaA"
                     /locus_tag="VF516_08660"
     CDS             2523..>4028
                     /gene="guaA"
                     /locus_tag="VF516_08660"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEX8107789.1"
                     /translation="MAHQLILVLDFGSQYTQLIARRIREQSVYCEIHPFTLARDPELR
                     QIRAMRPVGIVLSGGPSSVYDPGAPTISPALYQLGVPILGICYGAQLTAHLLGGDVRP
                     ADRREYGRATVRVKHIGGVFRTASLGEELSVWASHGDLVQALPPGFVHTAESDNCAMA
                     GFASAERGIHCVLFHPEVVHTPRGAELLGNFLFGVCNAAGDWSMASFVDESIARIHHQ
                     VGDHGRVICGLSGGVDSSVAAALLHKAIGDRLTCIFVDNGLLRRGEREQVAAIFRDHF
                     KVDLRVVDAEQRFLAALTEVTDPEAKRKIIGREFIAVFEDEARQIAGASFLAQGTLYP
                     DVIESVSFKGPSHLIKSHHNVGGLPERMKLALVEPLRELFKDEVRQLGLELGLPRHMV
                     WRQPFPGPGLAVRCLGSLTRHRLDVLRAADAIIEEEIRAAELYESIWQSFGVLLPVRS
                     VGVMGDARTYEEALALRAVHSRDGMTADWVPLPYDLLGRISSRVINEVRGIN"
BASE COUNT          597 a         1389 c         1461 g          581 t
ORIGIN      
        1 ggtcggccgg cggaacgccg gtggtcggca gcgggccgtg ccggttcacc gtcgccacca
       61 cgccggcggg cgcggtggtc cggttcgacg atcagccgat ggggccatcg ccgatcacga
      121 tcgagggcag ctgcgacaag cacaaggtcg acgtcgcgca cgcccgctac cagagcgtga
      181 cgcggtgggt gacgctggcg gccgacagga cgcagcagct cgacatcagc ctgccgcggc
      241 cgatccacgc ggtcaccgtc acgtcgtccc cgcccggtgc ggagctgtcg atcgacggcc
      301 accacgccgg cacgacgccg acggtggtcc agatggccgg cttcgcgacc gtcaacctga
      361 cgttcaccaa gcccggcttc cagaccgtga ccaggaaggt ctacagcaag caggcgcagg
      421 atcgcgtgtc cgtgaagctc acgaagtgac gccgccggcg tcagtggtgg tcgtggtcgt
      481 gatcgcggac gacgacgttg ccgcgcgcgc gccagccgcc ctcgacccac acgtggccct
      541 tggcgcgccg ctcccagcgg ccctcgacgt agacctgctc cgggcgctcg cgctcgtagt
      601 agccgccgcg ccacgcccag cggccaccgt catggtgcca gtggccgtgg atccagagga
      661 agcccgggcg agaaaccacg acttcctcgc gcggcggcgg cggctcctca tcgaccacgt
      721 aggcgcctcc gctggcgtag cagccggtca tcgcaggtgc ggtcagcgca gcgccggcga
      781 cgagcagcgc tgcgagcatg tcgcgtaatg atgtcatgtg aaatctccgt tgatcgggga
      841 gcacagcaat cagcgcgcca gcgtcggatt cgctcggtca tgcgcacgct gaggcgatct
      901 cgctcacgcc gcggcgcgtt ccgcgaacgg aggcccgtgg atcggcaggt gagatctcat
      961 gacaaatcgg agagttgatc gccgggtagg tccggccggc cggttggccg cgggcaccgc
     1021 cctggcttgc cgcgatcaga ccccccgagt atggtcggcc atgtttgagg acctccgcga
     1081 ggcgctgacc ttcgacgatg tcttgctcgt gccgggatac agcgaggtca tcccgcgcga
     1141 cgtcgatgtc agcacccggc tgaccccggc gatcccgctg cggatgccgc tggtgtcctc
     1201 ggcgatggac accgtcaccg aggccgccac cgcgatccgc atggcccgcg agggcgggat
     1261 cggcttcatc cacaagaacc tgtcgatcga ggatcaggcg cgcgaggtca accgcgtgaa
     1321 gaaggcgcag tccggcatcg tggtcgatcc ggtgacgatc gccccgtcgc gcacgctgga
     1381 ggacgcgctc gcgctgatgc ggcattaccg gatcagtggc ctgccggtgg tcgatgccga
     1441 tcgccgcccg gtcggcatcc tgaccaaccg agacgtgcgg ttcgagaagc ggctgcagct
     1501 cccggtcggc gagatgatga ccaagaaccc ggtgacggtg aaggagggcg tgcagatcga
     1561 cgaggccaag gacctcctgc acaagcatcg gatcgagaag ctgctggtgg tcgaccgcga
     1621 gggccggctc aagggcctga tcacgatccg cgacatcgag caggccgagg cccacccgac
     1681 ggcggcgacc gacgagctcg gccggctgcg ggtcggcgcc gcggtcgggg tcggcggcga
     1741 tcgcgaggcc cggatcgagg cgctgctcgc cgccggctgc gacgtgatct gcatcgacac
     1801 cgcgcacggc cactccgccg gggtgctcga ggcggtgcag aagacccgca agcggttcgg
     1861 caagctccag ctggtcgcgg gcaacgtcgc gaccggcgac gcgacgcgcg cgctgatcga
     1921 gtgcggcgtc aacgcggtca aggtcggggt cggcccgggg tcgatctgca ccacgcggat
     1981 cgtcgccggc gtcggcgtgc cgcagctgac cgcgatcggc gacgcggtcg cggcggcgcg
     2041 cggctcgggc gtcgcgatca tcgccgacgg cggcatcaag tactcgggcg acgtcgccaa
     2101 ggcgctcgcg gccggcgcgg acaccgtgat gatcggcagc ctgttcgccg gcaccgacga
     2161 ggcgcccggc gagatcatcc tgtaccaggg ccggtcgtac aagacgtacc gcggcatggg
     2221 ctcgatcggc gcgatgaagg acggctcgaa ggaccgctac ttccagggcg acgtcgaggc
     2281 gccgcagaag ctcgtgcccg agggcatcga gggccgcgtg ccgtacaagg ggccgctgcg
     2341 cgagtcgctg taccagctgg tcggcgggct gcgcgcgtcg atgggctacc tcggctgcgc
     2401 cacgatcgcc gagctgcacg ccaaggcccg gttcgtcaag atctcgtcgg cggggctgcg
     2461 cgagagccac gtccacgacg tgatcatcac caaggaagct cccaactacc gcgtggagtg
     2521 acatggcgca ccagctgatc ctcgtcctcg acttcggttc gcagtacacc cagctgatcg
     2581 cgcggcggat ccgcgagcag tcggtgtact gcgagatcca tccgttcacg ctcgcccgcg
     2641 atcccgagct ccgccagatc cgcgcgatgc ggccggtcgg catcgtgctg tcgggcgggc
     2701 cgtcgtcggt ctacgacccg ggcgcgccga cgatctcgcc ggcgctgtac cagctcggtg
     2761 tgccgatcct ggggatctgc tacggcgcgc agctcaccgc gcacctgctg ggcggcgacg
     2821 tccggccggc ggaccgccgc gagtacggcc gggccacggt gcgggtcaag cacatcggcg
     2881 gcgtgttccg caccgcatcg ctcggcgagg agctgtcggt gtgggcgtcg cacggcgacc
     2941 tggtccaggc gctgccgccg ggcttcgtcc acaccgcgga gtccgacaac tgcgcgatgg
     3001 cgggcttcgc gtcggccgag cgcggcatcc actgcgtgct gttccacccc gaggtcgtcc
     3061 acaccccgcg cggcgccgag ctgctcggca acttcctgtt cggggtgtgc aacgcggcgg
     3121 gcgactggtc gatggcgtcg ttcgtcgacg agtcgatcgc gcggatccac caccaggtcg
     3181 gcgatcacgg ccgggtgatc tgcgggctgt ccggcggggt cgactccagc gtcgccgcgg
     3241 cgctgctgca caaggcgatc ggcgaccggc tgacctgcat cttcgtcgac aacggcctgc
     3301 tgcgccgcgg cgagcgcgag caggtcgcgg cgatcttccg cgatcatttc aaggtcgacc
     3361 tccgggtggt cgacgccgag cagcggttcc tggccgcgct caccgaggtg acggatccgg
     3421 aggccaagcg caagatcatc ggccgcgagt tcatcgcggt gttcgaggac gaggcccggc
     3481 agatcgccgg cgcgagcttc cttgcccagg gcacgctgta ccccgacgtg atcgagagcg
     3541 tgtcgttcaa ggggccgtcg cacctgatca agtcgcacca caacgtcggc gggctgcccg
     3601 agcggatgaa gctcgcgctg gtcgagccgc tgcgcgagct gttcaaggac gaggttcgcc
     3661 agctcgggct cgagctcggc ctgccgcgcc acatggtgtg gcgccagccg ttccccggcc
     3721 cggggctcgc ggtgcgctgc ctgggcagcc tgacccggca ccggctcgac gtgctgcgcg
     3781 ccgccgacgc gatcatcgag gaggagatcc gcgcggcgga gctctacgag tcgatctggc
     3841 agtcgttcgg cgtgctgttg ccggtgcgct cggtcggggt gatgggcgac gcgcggacct
     3901 acgaggaggc gctcgcgctg cgcgcggtgc actcgcgcga cgggatgacc gcggactggg
     3961 tgccgctgcc gtacgacctg ctcggccgga tctcgtcgcg cgtgatcaac gaggtccgcg
     4021 gcatcaac
//
LOCUS       DASVOR010000435         6180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208643,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000435 DASVOR010000000
VERSION     DASVOR010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6180
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208643"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VF516_08665"
     CDS             complement(<1..921)
                     /locus_tag="VF516_08665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8107790.1"
                     /translation="MKTRKLGKSGPTVGAIGLGCMGMSEFYGARDDTESIATIHHALE
                     LGVTLLDTSDMYGPHTNERLVGKAIEGRRDRVVLATKFGIVRTANERSINGRPEYVKA
                     ACDASLLRLGVDVIDLYYQHRVDPGTPIEDTVGAMADLVRAGKVRYLGLSEAAPDTLR
                     RACKVHPITALQTEYSLWTRDPEDGVLAACRELGVGFVAYSPLGRGFLTGQIKTFDDL
                     APDDFRRFSPRFQGENFARNFALVEHLTAVARQKRVTPGQLALAWVLSRGDDIVPIPG
                     TKRRTYLDENAAAVDVAFTAEELARIDAIAP"
     gene            1038..1958
                     /locus_tag="VF516_08670"
     CDS             1038..1958
                     /locus_tag="VF516_08670"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8107791.1"
                     /translation="MVWLLVVVLAVVVLLVVRWRLVHRVQSPLDPEPFEGNVYRVGEA
                     TVLERASRDPSMTVVCMHGYLENFHYFTEFYADPAVQLILISSADYHAPEPTATPAWP
                     RDVPGVPGTIEYDAAVLVQALEHLPRAENIRVHGHSRGGAVVLEASVLRPDLFTRVEV
                     VLEAPVLPGGQTRTPISAVTLFALPFIVPLWRKNPISKQNRALWGQLDDPRKRQVISA
                     LAFNPRHIRTMLANLVSIAAWVKRTDAKHLSAVRGKVIVPGDDRVLDPESMRASAERA
                     GDRLEIVSVPGSSHFVLFDEPRLFELRPRA"
     gene            complement(1973..3187)
                     /locus_tag="VF516_08675"
     CDS             complement(1973..3187)
                     /locus_tag="VF516_08675"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8107792.1"
                     /translation="MLSTIGSRGDVQPLVALAMALKALGQEIRLCVPPDFREWIEGLG
                     MPVTPIGPELRSTGKAIPAAARPTPEQWRGLMESTVATQFETIAAAARGCDLLVGATA
                     LQIAAPSVAEAMGIPYVFAAYCPAVLPSRHHAPPVLPMWGDTPAPARVDHRERWDQDA
                     RRWNEHWGSLLNAGRASLGLAAVPDVRSHVLTDRPWLAADPTLAPWPEPGDPAVVQTG
                     AWILPDERPLSPELQAFLDAGEPPVYFGFGSIRAPEDLGRVMIESARALGRRAIVLRG
                     WADLSLLDDGPDCLAIGEVNQQALFQRVAAVVHHGGAGTTTAAARAGAPQVVIPQHYD
                     QHYWAQRIHDLGIGTAHASGTPTTSSLTSALARSLEPDLAARARSIATAVRSDGAQRA
                     ARQLIRGAPRPG"
     gene            3367..3828
                     /locus_tag="VF516_08680"
     CDS             3367..3828
                     /locus_tag="VF516_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107793.1"
                     /translation="MRVLFCGVLRIAILVAALCGVARADEVMYWCDGDANVKVTIARG
                     GTCGSFDARDASGKVVQHGAFDFTASGHLYATADGHTVVYLAGSPGDLGTIAKPAVAK
                     GDVKGALTFQIAKGVALTDDTSGITVCLTGSPRGWLAIDKLDVMYNALPTR"
     gene            4029..5267
                     /locus_tag="VF516_08685"
     CDS             4029..5267
                     /locus_tag="VF516_08685"
                     /inference="COORDINATES: protein motif:HMM:NF024241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8107794.1"
                     /translation="MAGIRRRIAEVGSVAPRAYWLVWWGTLINKLGGFVVPLLTIYLV
                     TVRRLSVSDAGGIVSVFGAGAILASLTGGYLADRLGRKVTLLISLFGGAIALTLLGLV
                     RGLTAITAMVGVVGFVGELYRPAVHALVADVVPPSHRVRAYGLLHWVINIGFSFAAIM
                     GGLLASVDFAILFIADAATMAIYGIIVLVAVPETRPARIARGPEHAPSQSWLEDREFV
                     VFVAITFAAVLLPYQANAPLSAHMTWQGFTPAAFGAVLSVNGILIIALQPAISTWSFR
                     REPTRVLIAAAAFYGIGLAMHGLATTVLVHGIAVMVWTIGEILESPVRATVVAAMAPA
                     DSRGRYQGAFAMTFGAGQLVGPKLGTWIWQHEGPDVLWGSCLGLSLVVALALIATGPA
                     RRRRMAATPALVGSSSTSST"
     gene            5387..5773
                     /locus_tag="VF516_08690"
     CDS             5387..5773
                     /locus_tag="VF516_08690"
                     /inference="COORDINATES: protein motif:HMM:NF015898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HEX8107795.1"
                     /translation="MRKKPDPLTRREREIMDILHRRGRATAHEVMDDLADPPSYSAVR
                     TFLRLLEERGHVRHEQDGPRYVYTPTVARREAQRSAITHLVDTFFDGSVEDAVATLVE
                     SSKPKLSAQELDRIAALIAKAKKEGR"
     gene            5775..>6180
                     /locus_tag="VF516_08695"
     CDS             5775..>6180
                     /locus_tag="VF516_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107796.1"
                     /translation="MTILSPILLIARGAIVLALALGATRLLSRAPASLRFTLLASALA
                     AVFALPILEALVPAWYAGAIPADTVEIVEPPALPVAEAAAPENVHAASVAGRPAESFA
                     VPWRAIFGGLWAAGIAAMLLRVGVGVLRARRIA"
BASE COUNT          849 a         2142 c         2197 g          992 t
ORIGIN      
        1 gggggcgatc gcgtcgatgc gcgcgagctc ctcggcggtg aacgcaacgt ccacggccgc
       61 ggcgttctcg tcgaggtacg tgcgccgctt ggtgcccggg atcgggacga tgtcgtcgcc
      121 gcgcgacagc acccatgcga gcgcgagctg gcccggcgtg acgcgcttct gccgggcgac
      181 ggccgtcagg tgctcgacca gcgcgaagtt cctggcgaag ttctcgccct ggaacctggg
      241 ggagaaccgg cggaagtcgt ccggagcgag gtcgtcgaac gtcttgatct ggccggtgag
      301 gaagccgcga ccgagcgggc tgtacgcgac gaagccgacg ccgagctcgc ggcacgcggc
      361 cagcacgccg tcctcggggt cacgcgtcca cagcgaatat tcggtctgca gcgccgtgat
      421 cgggtgcacc ttgcaggcac ggcgcagcgt gtccggcgcg gcctcggaca ggccgaggta
      481 gcggaccttg cctgcgcgca cgaggtcggc catcgcgccg accgtgtcct cgatcggcgt
      541 ccccggatcg acgcggtgct ggtagtagag atcgatcacg tcgacgccga gccgcaggag
      601 gctggcatca cacgccgcct tgacgtactc ggggcggccg ttgatcgagc gctcgttggc
      661 ggtgcgcacg atgccgaact tcgtcgcgag gacgacgcga tcgcggcggc cctcgatcgc
      721 cttgccgacc agccgctcgt tggtgtgggg gccgtacatg tccgaggtgt cgagcagcgt
      781 gacgccgagc tcgagggcgt gatggatcgt cgcgatcgac tcggtatcgt cgcgagcacc
      841 atagaactcc gacatcccca tgcagcccag accgatcgcg ccgacggtcg gtccggactt
      901 gccaagcttg cgggtcttca tgcccgagaa cttgccgcga gcccctgcga tccacaagct
      961 cggatccgac cagaacacac gtcgaatcca atgggtgtga tgccggatgg ccggcgtcgt
     1021 gacgtatcct gcgcggcatg gtgtggctcc tcgtcgtcgt gctcgccgtg gtggtcctgc
     1081 tcgtggtgcg atggcgcctc gtgcaccgcg tccagtcgcc gctcgacccc gagccgttcg
     1141 agggcaacgt ctaccgcgtc ggggaggcga ccgtcctcga gcgagccagc cgggacccga
     1201 gcatgaccgt ggtgtgcatg cacggctacc tcgagaactt ccactacttc accgagttct
     1261 acgccgatcc ggcggtccag ctcatcctga tctcgagcgc cgactaccat gcgccggagc
     1321 cgaccgcgac gccggcgtgg ccgcgcgacg tgcccggcgt ccccggcacc atcgagtacg
     1381 acgcggccgt gctggtccag gcgctcgagc acctgccccg ggccgagaac atccgcgtgc
     1441 acggccactc ccgcggcggc gcggtcgtgc tcgaggcgtc ggtgctgcgg ccggatctgt
     1501 tcacccgcgt cgaggtggtg ctcgaggccc cggtgctccc cggaggccag acccgcacgc
     1561 cgatctccgc ggtgacgctc tttgcactgc ccttcatcgt gccgctgtgg cgaaagaacc
     1621 cgatctcgaa gcagaaccgc gcgctgtggg ggcagctcga cgatccgcgc aagcgtcagg
     1681 tgatcagcgc cctggcgttc aacccgcgcc acatccgcac catgctcgcc aacctcgtgt
     1741 cgatcgcggc ctgggtgaag cgcaccgatg cgaagcacct gagcgccgtg cgcggcaagg
     1801 tcatcgtccc gggcgacgac cgcgtcctcg atcccgaatc gatgcgagcc agcgccgagc
     1861 gggccggcga tcggctggag atcgtgtccg tcccgggatc gagccacttc gtgctgttcg
     1921 acgagccgcg gctgttcgag ctgcgcccgc gcgcatagtc gctgccgccg tgctacccgg
     1981 ggcgtggcgc gccgcggatc agctgtcgag cggcgcgctg tgcaccgtcg ctgcgcacgg
     2041 ccgtggcgat cgatcgggca cgggcggcca ggtcgggctc gaggctgcgt gcgagcgcac
     2101 tcgtgagcga gctcgtggtc ggtgtgccgg atgcgtgggc ggtcccgatg ccgaggtcgt
     2161 ggatgcgctg ggcccagtag tgctggtcgt aatgctgcgg gatcaccacc tgtggcgcgc
     2221 cggcgcgcgc ggccgcggtg gtggtgccgg caccgccgtg gtggacgacg gctgcgactc
     2281 gctggaacag cgcctgctgg ttgacctcgc cgatggccag gcagtcgggt ccgtcatcga
     2341 ggagggacag gtcggcccac ccgcgcagca cgatcgctcg gcgcccgagc gcgcgggcgg
     2401 attcgatcat cacacggccg aggtcctccg gcgcacggat gctgccgaag ccgaagtaga
     2461 cgggcggctc tccggcatcg aggaacgcct gcagctcggg cgagagcggg cgctcgtccg
     2521 gcaggatcca ggcgccggtc tgaaccacgg ccgggtcccc gggctcgggc cagggcgcca
     2581 gcgtcgggtc ggcagccagc cagggccggt cggtcaggac gtggctgcgc acgtcgggga
     2641 ccgcggccag gcccagcgag gcgcgacccg cgttgagcag cgagccccag tgttcgttcc
     2701 agcgccgcgc atcctggtcc cagcgctcgc ggtggtcgac cctcgcgggc gccggtgtgt
     2761 cgccccacat ggggagcacg ggtggcgcgt ggtgccgcga tggcaggacc gccgggcaat
     2821 acgcggcaaa gacgtacggg atgcccatcg cctctgccac ggacggagcg gcgatctgca
     2881 gggccgtcgc tcccacgagg aggtcgcagc ctcgcgctgc cgccgcgatc gtctcgaact
     2941 gcgtggccac cgtgctctcc atcagcccac gccactgctc gggcgtgggt cgggcggctg
     3001 ccgggatcgc tttcccggtc gagcgcagct cgggtccgat cggcgtgacg ggcatcccca
     3061 ggccctcgat ccactcccgg aagtcgggcg gtacacacag gcgaatctcc tggccgagcg
     3121 ccttcagcgc catcgccagc gccaccagcg gctggacatc gccccgcgat ccgatcgtcg
     3181 atagcaacgc gcgcatcaag ctccccttcg ctcggtgtgg acggtttgca tcacacgcac
     3241 gtgatcgcga acttcgacgt acgttccaag cttgtcactg cgggctggtt ttgcgagtat
     3301 gtgagtggga agagggcgta cggaggcccg cctcgagggc ggtgttcgcg gggctcgatg
     3361 ccggcggtgc gggtgctatt ctgcggtgtg ctccgcatcg ccatcctggt cgccgcgctg
     3421 tgcggcgtcg cgcgcgccga cgaggtcatg tactggtgcg acggtgacgc caacgtcaag
     3481 gtcacgatcg cccgcggcgg cacgtgcggc agcttcgacg cccgcgacgc gagcggcaag
     3541 gtcgtccagc atggtgcgtt cgacttcacg gcgagcggcc acctgtacgc gaccgccgac
     3601 ggccacacgg tggtgtacct cgccggcagc ccgggcgacc tcggcacgat cgccaagccc
     3661 gcggtcgcca agggggacgt caagggcgcg ctgaccttcc agatcgccaa gggcgtcgcg
     3721 ctcaccgacg acaccagcgg catcaccgtg tgtttgaccg gctcgccgcg cggctggctc
     3781 gccatcgaca agctcgatgt gatgtacaac gcgctcccca cccgctgaag cgggccccgt
     3841 gccggctacc tgcggccgac gaaacagatc gagctgatcc gggacctggc ggagcgggga
     3901 tccgcgtcgt gcctgccctc gacacggcac cggcccggca tgccggccct gtgacgccgc
     3961 gggccagctc ttcgtgtgtg ggccgcgcgc tggcaacacg cgcgcgatgc tctacgctcc
     4021 cgaccgtggt ggcaggaatt cgacgccgga tcgccgaggt ggggtcggtc gcgccgcgcg
     4081 cctactggct cgtatggtgg ggaacgctga tcaacaagct cggcggcttt gtcgtcccgc
     4141 tgctgacgat ctatctggtc acggtccgcc gtctcagcgt cagcgatgcc ggtggcatcg
     4201 tctcggtgtt cggcgccgga gcgatcctgg cgtcgctgac cggggggtac ctggccgacc
     4261 gcctcggccg caaggtgacg ctgctgatct cgctgttcgg tggcgcgatc gcgctgacgc
     4321 tgctcgggct ggtccgcggg ctgacggcga tcaccgcgat ggtcggcgtg gtcggcttcg
     4381 tcggcgagct gtaccgcccg gcggtgcacg cgctcgtcgc ggacgtcgtg cccccgtcac
     4441 atcgcgtgcg ggcgtatggc ctgctccact gggtcatcaa catcggcttc tcgtttgccg
     4501 ccatcatggg cgggctgctc gccagcgtcg acttcgcgat cctgttcatc gccgatgcgg
     4561 cgaccatggc gatctacggc atcatcgtgc tcgtcgcggt gcccgagacg cgccctgccc
     4621 ggatcgctcg cggacctgag cacgcgccga gccaatcatg gctggaggat cgcgagttcg
     4681 tcgtgttcgt cgcgatcacg ttcgcggcgg tactcttgcc gtaccaggcg aacgcgccgc
     4741 tgtcggcgca catgacctgg caaggcttca caccggcggc gtttggcgcc gtcctgtcgg
     4801 tcaacggcat cctgatcatt gcgctgcagc cggcgatctc gacctggagc tttcgccgcg
     4861 agccgacgcg cgtgctgatc gccgccgcgg cgttctatgg catcggtctg gcgatgcatg
     4921 gcctggcgac cacggtcctg gtgcacggca tcgcggtgat ggtgtggacg atcggcgaga
     4981 tcctcgaatc gccggtgcgc gcgacagtgg tcgccgcgat ggctccggcg gactcccgcg
     5041 gccgctacca gggcgcgttc gcgatgacgt ttggcgccgg gcagttggtc ggccccaagc
     5101 tgggtacgtg gatctggcag cacgagggac cggacgtgct gtgggggtcg tgcctggggc
     5161 tcagcctcgt cgtggcgctc gcgctgatcg cgaccgggcc tgcccgccgc cgccgcatgg
     5221 ccgccacgcc tgcgctcgtt ggctcgtcat cgacaagctc gacgtgatgt acacgcgctt
     5281 tccacgcgat gaagcgcgct tgtctggctc atccccagat cgatgtgcga aaaaaacagc
     5341 accccgccct tgctaaagga ttagcagtag gctaaatctt tagcacgtga ggaagaaacc
     5401 cgatccgctg acgcgccgcg agcgcgagat catggacatc ctgcaccgac gcgggcgcgc
     5461 gaccgcacac gaggtgatgg acgatctcgc cgatccgccg agctattcgg cggtgcgcac
     5521 gttcctccgc ctcctcgagg agcggggcca cgtgcgacac gagcaggacg gtccgcgcta
     5581 cgtctacacg cccaccgtcg cgcgccgcga ggcgcagcga tccgccatca ctcacctcgt
     5641 cgacacgttc ttcgacggct cggtcgagga cgcggtcgcg acgctggtcg agtcctcgaa
     5701 gccgaagctg tccgcgcagg agctggatcg catcgccgca ctcatcgcca aggccaagaa
     5761 ggagggccgc tgatatgacg attctctccc cgatcttgct gatcgcgcgt ggcgcgatcg
     5821 tgctcgcgct cgcgctcggc gcgacgcggc tgctgagccg cgcaccggcg tcgctgcgct
     5881 tcaccctcct cgcgtcggcg ctggccgccg tgttcgcgct cccgatcctc gaggcgctcg
     5941 tcccggcgtg gtacgcggga gcgatccccg ccgacacggt cgagatcgtc gagccgcctg
     6001 cgctgccggt cgccgaggcc gcggcgccgg agaatgtaca cgcggcgagc gttgcaggcc
     6061 ggccggcgga gtcgttcgcg gtcccgtggc gcgcgatctt cggcgggctg tgggcggcag
     6121 gcatcgccgc gatgctgctc cgcgtgggcg tcggcgtgct gcgtgcgcgt cgcatcgcgc
//
LOCUS       DASVOR010000436         2473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266855,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000436 DASVOR010000000
VERSION     DASVOR010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266855"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..993)
                     /locus_tag="VF516_08700"
     CDS             complement(211..993)
                     /locus_tag="VF516_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107797.1"
                     /translation="MRFTDLVRLLRSLDLADRGAHGRRPPRDHVRTVQDALTAMLADE
                     DFYLDCIDLEAETTASETPDRPFAPLVRLNDLELQFRMFYWWPGKAAPPHEHTAWTVT
                     AVFYNTLEVTTFDWDVAFREQRLQPKNRFTGAQGMAGHIYDRCIHRPANLTEKLSTSI
                     HIFHAADQPRIEREVGPIAGMSPPTRAFVRGPNEEARLTALKQLRLRALAHMVGQFHS
                     ERARRTLDAIASWGDDLTRAVTDRVTWSMSRGTDPRVASEGR"
     gene            1129..>2473
                     /locus_tag="VF516_08705"
     CDS             1129..>2473
                     /locus_tag="VF516_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8107798.1"
                     /translation="MDSITLLGDRLRAHPTGARAVHDQRWWTYGELVTAAEPIARRIA
                     ELGLGRGDVVALPIDRGIEAVVATCATLLAGAIPAVLDPRDPDTAARTIARLRPALVL
                     VATDHAGLPGAHVVELRGGRGELSAATPPVSDPVRRDPRISHVIFTSGSTAETKGVIW
                     SELRASFDGSAGAPQASHRGRDPRRPGGIHASLCTGLGFHELLHALHHGNAVALLHAP
                     FLATLAQIRELGVDRLRLTPTHVEMLLATAELLPEVAAVSVATAPIAPERLLALRARV
                     PNARLCRAYGLTETGPATAVWSDRNPRKLHTVGRAVAFRRITVRDPDGNMLPPDRPGE
                     VVIELPMWDACDGYLDAPPELARRFTNGVLWTGDRGTIDRHGFLVLAGRQAEVLKVGG
                     RSVSAPRIEQALGAMPGVRELAVVGVPDRWLGEVPCAVYVPDSGADSGADSGADSG"
BASE COUNT          342 a          892 c          878 g          361 t
ORIGIN      
        1 cgccacatcc gccacacccc ccgcagccac cgcacccgcc acccgcgccg caggtcgcgc
       61 cgcaatcgtc gaacgacgcc tcgaacggtg ccacgtgagg atcgagcacg gcggcgagtg
      121 acaccggctt cgaggaaccg ccggcggggg tccgccagcg gtccgcgagc tgtgcagccg
      181 tcaatccacc gaacagcgct ggtagctccg tcatcggccc tccgaagcga cccgggggtc
      241 ggttccgcgt gacatcgacc acgtgacacg atccgtcacc gcccgggtga gatcgtctcc
      301 ccagctcgcg atcgcgtcca gcgttcgtcg cgcgcgctcc gagtggaact ggccgaccat
      361 gtgagcgagc gcacggagcc gcagctgctt gagcgcggtg agtcgtgctt cctcgttcgg
      421 cccgcggacg aacgctcggg tcggtgggga catcccggcg atcgggccga cctcgcgctc
      481 gatccgaggc tgatcggcgg cgtggaagat gtggatcgag gtcgagagct tctcggtgag
      541 gttcgccggg cgatggatac agcggtcgta gatgtgaccg gccatgccct gcgcccccgt
      601 gaagcgattc ttgggctgca gccgctgctc gcggaacgcg acgtcccagt cgaacgtcgt
      661 gacctcgagc gtgttgtaga acacggccgt caccgtccac gccgtgtgct cgtgcggcgg
      721 agctgccttg cccggccacc agtagaacat ccggaactgg agctcgaggt cgttcaggcg
      781 aaccagcggg gcaaacggtc gatccggtgt ctcgctcgcc gtcgtctcgg cctcgaggtc
      841 gatgcagtcc agatagaagt cctcgtcggc gagcatcgcc gtcaaggcgt cctggacggt
      901 gcggacgtgg tcgcgcggag gccggcgccc gtgcgcgcct cgatcggcga ggtcgagcga
      961 gcgcagcagg cggacgagat ccgtgaacct catgatcccg aagacctccg cggggtcgca
     1021 cctcggccca ggttgggggt catccgaagg atatctttcc agatctgcac tgcgccgggc
     1081 aacctgataa cgtcgtccga ccgaacgcgt cacaacggtt cgggtatcgt ggactccatc
     1141 actctgctgg gcgatcggct gcgcgcgcac ccgacgggtg ctcgggcggt gcacgaccag
     1201 cggtggtgga cctacggcga gctggtgacc gcggcagagc cgatcgcccg gcggatcgcc
     1261 gagctcggcc tcggccgagg tgacgtcgtc gcgcttccca tcgatcgcgg catcgaggcc
     1321 gtggtggcga cgtgcgcgac cctgctcgcc ggagcgatcc ccgcggtgct cgatccgcgc
     1381 gaccccgata ccgcggcgcg cacgatcgcg cggctacgcc ccgcgctcgt cctggtcgcg
     1441 acggaccacg cgggcctgcc gggcgcgcac gtggtcgagc tgcgcggcgg gcgtggcgag
     1501 ctatcggcgg cgacccctcc ggtctccgat ccggtgcggc gagatccgcg catctcgcac
     1561 gtgatcttca cctcgggctc gaccgccgag accaagggcg tgatctggag cgagctgcgc
     1621 gcgagcttcg acgggtcggc cggcgcgccg caggcaagcc accggggccg agatccacgc
     1681 cgccccggcg gcatccacgc ctcgctgtgc accgggctgg gcttccacga gctcctccac
     1741 gcgctccatc acggcaacgc ggtcgcgctg ctccacgcgc cgttcctggc gacgctcgca
     1801 cagatccgcg agctcggggt cgatcgcctg cgcctcacgc cgacccacgt cgagatgttg
     1861 ctcgcgaccg ccgagctgct tcccgaggtc gcggcggtgt cggtcgcgac cgcgccgatc
     1921 gcgcccgagc ggctcctcgc gctgcgcgcg cgggtgccga atgcacggct gtgccgagcc
     1981 tacggcctga ccgagaccgg tcccgcgacc gcggtatggt ccgatcggaa cccgcgcaag
     2041 ctacacaccg tcggccgcgc cgtcgcgttc cgccggatca cggtgcgcga tcccgacggc
     2101 aacatgctgc cgccggatcg accgggcgag gtcgtgatcg agctgccgat gtgggacgcg
     2161 tgcgacggct acctcgacgc gccgcccgag ctcgcacggc ggttcaccaa cggcgtgctg
     2221 tggaccgggg atcgcgggac gatcgatcgc cacggcttcc tcgtgctcgc gggtcgccag
     2281 gccgaggtgc tcaaggtcgg cggacgcagc gtgagcgcgc cccggatcga gcaggcgctc
     2341 ggtgcgatgc cgggcgtgcg cgagctcgcc gtcgtcggcg tgcccgatcg ctggctcggc
     2401 gaggtcccgt gcgccgtcta cgttccggac tccggcgcgg actccggcgc ggactccggc
     2461 gcggactccg gcg
//
LOCUS       DASVOR010000437         4225 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_33183,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000437 DASVOR010000000
VERSION     DASVOR010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4225
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33183"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VF516_08710"
     CDS             <1..351
                     /locus_tag="VF516_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107799.1"
                     /translation="ATSSAAVLDQTQRYAKDSVIVRFRSAPTAPFARTLATRVNGAIE
                     DRDYPSVFRAQETAYRWSVCEQAGLCSNEVSVDFGPDPAGDQATMTTEIGGTQVVEGK
                     PSLGVHRDAIASGP"
     gene            complement(332..1825)
                     /locus_tag="VF516_08715"
     CDS             complement(332..1825)
                     /locus_tag="VF516_08715"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HEX8107800.1"
                     /translation="MASPVLQLEGSLDPSAIPPDVATELRCYLKVRAAAALGPTTKAL
                     VANICLVLDCSGSMVGEKAEAAIEAAKRIVEIVDERHRISLVGFALSSRVIVDNAQAT
                     PAGRDAIKAKIDRLRGMFGGGTVLGSGIRAGADLVQRFGADAAILVVLSDGAADDRSE
                     ALAQAVRARELGIQLFAVGIGDDYEADHLRKLVTPSSGAVLAPGRLDQLAATFGELAA
                     RIERFVATSAQLAVTLGEGVRARQVYRALPQPAFIGDASAGTAREVELRVGNVERGQT
                     HAFLLAIVVPPHVDPALEVARATLTFDVPALGLRAQHQAIALHVARGADALTGNAEIS
                     GAYRSAQIARLVDELADAQRRHAKDECLDRVDLLIRLSDEQGDDRARASYEQLRDGIA
                     GDRAIAQRALNELVIASTEHKPTAADASSAIKQLYDVVLVAPGDAPILLLRALRDATG
                     KSLRELNDLIAMTPVAIREALPRRDAEALVTQIEASGTGAVLKVRKR"
     gene            complement(1831..3132)
                     /locus_tag="VF516_08720"
     CDS             complement(1831..3132)
                     /locus_tag="VF516_08720"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein L7/L12"
                     /protein_id="HEX8107801.1"
                     /translation="MTTLLSRAPRIAATSLRGGVLALLMKLRQRPRTAAERESLHRIV
                     ASLGKLKGAAMKLGQHMSYFDTTLPEDVRAALAALQTHSPPMAVSRVTKILRSELGEA
                     ASPILDSLEPAPLASASIGQVHRARLSDGTRVAVKVQYPGIAAAIKTDFGPASVVGRF
                     ASWMHPSSPVESFLREAKACALAECDYRAEARQQADLAARYDDHPRIAVPTVHASYCT
                     ARVIVTTYAEGSHLGAWLATGPSQEQRDAIGEALVAFYVGSALRWGVLPGDPHPGNYL
                     VLPDGRLAIVDHGCTRTVPASRARLVLEAEDAAAAQRAAAGLAADALLLLRVRLGIAS
                     VLAQLGVHTPWRALVAKAMAPVTAGPVATRFDVVLVAPGERMIEIVRDVRDLMGADIR
                     NAKELLEQAPCVLRTTSDRSEAEALKHRLETSGATVEIRAS"
     gene            3227..>4225
                     /locus_tag="VF516_08725"
     CDS             3227..>4225
                     /locus_tag="VF516_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107802.1"
                     /translation="MRACSSLAVALLVPSVALAAPAASEDAPRVAVNVVIDLAPVPGV
                     PAWFASAFEQTVARELAGFERLGTVAKQDVALDACGTDRACRLRTYRGAGVDIALFGS
                     VTDDTIQYELYQTWTPARLESGAIAIDRHQSLVALEHATRDAFHVVLKHGGLLEQKPY
                     LLEREPTTASATGAAWSARALEMLLAVLALLVLPFALALLGGEQPATIVAMRSARRVA
                     LVIAAGFATVIALDPRRIGELVTAWAPLVAGVGGLGWGAFLVLVVRTAFPPLDGLERV
                     PQRELGRFLSTWCLAAIERLAVLAISNAPLIVLVAWLGASLAIAPQWIYLVLAPAVV"
BASE COUNT          568 a         1505 c         1520 g          632 t
ORIGIN      
        1 gcgacgagct cggccgccgt actcgaccag acgcagcgct acgcgaagga ctcggtcatc
       61 gtgcgcttcc ggtcagctcc caccgcgccg ttcgcacgca cgctcgccac gcgggtgaac
      121 ggtgccatcg aggacaggga ctacccgtcg gtgttccgag cgcaggagac tgcctatcgc
      181 tggtccgtct gcgagcaggc tggcctgtgc tcgaacgagg tgagcgtcga cttcggtccg
      241 gacccggcgg gcgatcaggc gacgatgacg accgagatcg gaggcacgca ggtcgtggag
      301 ggcaagccga gcctcggtgt ccatcgcgac gctatcgctt ccggaccttg agcaccgcgc
      361 cggtcccgga ggcttcgatc tgggtgacga gcgcctcggc gtcgcggcgc ggcagcgctt
      421 cccggatcgc gaccggggtc atcgcgatca ggtcgttgag ctcgcgcagc gacttgccgg
      481 tggcgtcgcg cagtgcgcgg agcaggagga tcggcgcatc gccgggcgcg accagcacga
      541 cgtcgtagag ctgcttgatc gcgctcgacg cgtcggccgc cgtcggcttg tgctcggtgc
      601 tcgcgatgac gagctcgttg agcgctctct gcgcgatcgc gcgatcgccg gcgatgccgt
      661 cgcggagctg ctcgtacgac gcccgcgcgc ggtcgtcgcc ctgctcgtcg gaaagtcgga
      721 tcaggagatc gacccggtcg aggcattcgt ccttcgcgtg gcggcgctgc gcgtcggcga
      781 gctcgtcgac caggcgcgcg atctgcgcgc tgcggtaggc accactgatc tcggcgtttc
      841 cggtcagcgc atcggcgcca cgcgccacgt gcagcgcgat cgcctggtgc tgggcgcgca
      901 gaccgagcgc gggcacgtcg aacgtgagcg tcgcgcgcgc gacctcgagc gcgggatcga
      961 cgtgcggtgg cacgacgatc gcgagcaaga acgcgtgggt ctggccgcgc tcgacattgc
     1021 cgacccggag ctcgacctcg cgcgctgtcc ccgccgacgc gtcgccgatg aacgcgggct
     1081 gcgggagcgc ccggtagacc tggcgcgcgc gcacgccctc accgagcgtc accgcgagct
     1141 gcgcgctggt cgcgacgaag cgctcgatgc gcgcggccag ctcgccgaag gtcgcggcca
     1201 gctggtcgag acggcccggg gcgagcaccg cgccgctcga cggcgtcacg agcttgcgca
     1261 ggtggtctgc ctcgtagtca tcgccgatcc cgacggcgaa cagctggatc ccgagctcgc
     1321 gcgcgcgcac cgcctgggcg agcgcttcgc tccggtcgtc ggccgcgccg tccgacagga
     1381 cgacgaggat cgcggcgtcc gcaccgaagc gctggaccag atcggccccc gcgcggatcc
     1441 cgctgccgag caccgtgccg ccgccgaaca tcccgcgcag ccggtcgatc ttcgccttga
     1501 tcgcgtcgcg gccggccggc gtcgcctgcg cgttgtcgac gatcacgcgc gagctcaggg
     1561 caaagccgac cagcgagatc cggtggcgct cgtcgacgat ctcgacgatc cgcttcgccg
     1621 cctcgatcgc cgcctcggcc ttctcgccca ccatcgagcc gctgcagtcg agcacgaggc
     1681 agatgttcgc gacgagcgcc ttcgtcgtgg gcccgagcgc cgccgcggcg cgcaccttca
     1741 ggtagcagcg cagctcggtc gcgacatccg gcgggatggc cgacggatcg aggctgcctt
     1801 cgagctggag gacgggcgag gccatcgccg ctagctcgcc cggatctcga cggtggcccc
     1861 cgaggtctcg agccggtgct tgagcgcctc ggcttcgctg cgatcggagg tcgtccgcaa
     1921 cacgcacggt gcttgctcga ggagctcctt cgcgttgcgg atgtccgcgc ccatcagatc
     1981 gcgcacgtcg cgcacgatct cgatcatccg ctccccgggc gcgaccagca cgacgtcgaa
     2041 tcgcgtcgcg accggtccgg ccgtgaccgg cgccatcgcc ttcgccacga gcgcgcgcca
     2101 cggggtgtgc acgccgagct gggcgagcac cgacgcgatc cccaggcgca cgcgcagcag
     2161 cagcagcgca tcggccgcga gaccggcggc ggcgcgttgt gcggcggccg cgtcctcggc
     2221 ttcgagcacg agccgcgcgc gtgacgcggg gaccgtgcgc gtgcagccgt gatcgacgat
     2281 cgcgaggcgg ccgtcgggca ggacgaggta gttaccggga tgcggatcgc cggggagcac
     2341 gccccagcgc agcgccgagc ccacatagaa cgccacgagc gcctcgccga tcgcgtcgcg
     2401 ctgctcctgg ctcgggccgg tcgcgagcca tgcgccgaga tgactgccct cggcgtacgt
     2461 cgtgacgatc acgcgggccg tgcagtacga agcatgcaca gtggggacgg cgatccgtgg
     2521 gtggtcgtcg tagcgggccg cgaggtccgc ttgctggcgc gcctccgcac ggtaatcgca
     2581 ttcggcgagc gcgcacgcct tcgcttcgcg caggaagctc tccaccggtg acgacggatg
     2641 catccagctg gcgaacctgc cgaccaccga ggccggaccg aagtcggtct tgatcgcggc
     2701 ggcgatgccc gggtactgca ccttgaccgc gacgcgcgtg ccatcggaga gcctcgcacg
     2761 atgcacctga ccgatcgagg ccgaggccag cggcgccggt tcgagcgagt cgaggatcgg
     2821 cgaagcggcc tctcccagct cggaccgcaa gatcttggtg actcgcgata cggccatcgg
     2881 cggagagtgg gtctgcagcg cggcgagcgc cgcgcgcacg tcctcgggca acgtcgtatc
     2941 gaagtacgac atgtgctgtc cgagcttcat cgcagcaccc ttgagcttgc cgagcgatgc
     3001 gacgatccgg tgcagtgatt cgcgctcggc ggcggtccgc ggtcgctggc gcagcttcat
     3061 cagcagcgcg agcacgccgc cgcgcaacga tgtcgccgcg atgcgcggtg cgcgtgacag
     3121 caacgtggtc acgatgtcgg cgtgtgacgc caagagtcgc acctccgtct cgcgtcttcg
     3181 ctgctcccga gtgtggtggt ccatggagtt ctagtaccat gccaccgtga gggcttgctc
     3241 gtcgctcgcc gtcgcgctgc tggtgccttc ggtcgcactc gccgcgccgg cggcttccga
     3301 ggacgcacca cgcgtcgcgg tgaacgtcgt gatcgacctc gcgccggtgc cgggagtccc
     3361 cgcgtggttc gcgagcgctt tcgagcagac ggttgcgcgc gagctcgccg ggttcgagcg
     3421 cctcggcacg gtcgcgaagc aggacgtcgc gctcgacgca tgcggcaccg atcgcgcgtg
     3481 ccggctgcgc acgtaccgcg gcgccggcgt cgacatcgcg ctgttcgggt cggtcacgga
     3541 cgacacgatc cagtacgagc tgtatcagac ctggacgccg gcgcgcctcg aatccggagc
     3601 gatcgcgatc gaccgtcacc agagcctggt cgcgctcgag cacgcgaccc gcgatgcgtt
     3661 tcacgtcgtg ctcaagcacg gcgggctcct cgagcagaag ccctacctgc tcgagcgcga
     3721 gccgaccacc gcgagcgcga ccggcgcggc atggagcgca cgcgcgctcg agatgctgct
     3781 cgccgtgctc gcgctcctgg tcctgccgtt cgcgctcgcc ctgctcggcg gtgagcagcc
     3841 cgcgaccatc gtcgcgatgc gctcggcgag gcgcgtcgcg ctcgtgatcg cggcgggctt
     3901 cgccacggtc atcgcgctcg atccacggcg gatcggcgag ctcgtcaccg cgtgggcacc
     3961 gctggtcgca ggggtcggtg gactgggctg gggcgcgttc ctcgtgctcg tcgtgcgcac
     4021 ggcgtttccc ccgctcgacg ggctggagcg cgtgccgcaa cgcgagctcg gccggttcct
     4081 gtccacctgg tgtctcgccg cgatcgagcg gctcgccgtg ctcgcgatca gcaacgcacc
     4141 gctgatcgtg ctcgtcgcgt ggctcggggc gtcgctcgcg atcgcgccgc agtggatcta
     4201 cctggtgctg gcgccggcgg tcgta
//
LOCUS       DASVOR010000438         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_266880,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000438 DASVOR010000000
VERSION     DASVOR010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_266880"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="VF516_08730"
     CDS             complement(<1..1271)
                     /locus_tag="VF516_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107803.1"
                     /translation="MRTARVSWPALAVLALAGGCKVADKHPDEPDAGPGDAGGGPVDT
                     HAPDTRIDQAPEAFSNSGQAAFRFSSDDPSATFECRIDQEAAQPCQSPYVRTLPDGPH
                     SFSVRAVDPAGNSDDTPAERVWTIDTVPPDTILLTGPPAADNSVTAQFTFRSSEDNVS
                     FDCSVDNAGYLPCTSGQSFALAGDGPHSFAVRAHDRAGNLDASPAIYAWTVDTRTPDT
                     QIVGGPPAASPSTTATFTFVSPDAGAGATFQCSLDGAAFTACSSPQTYSGLREAAHGF
                     AVRVRDAVGNFDPTPATRSWTVDLTPPDTAITAGPSGTVPSASASLSFSSSEPDSTFS
                     CSLDGAPFAACSSPAALTGLAQGAHTFAVRATDAAGHDDPTPATRSWTVDTVAPDVAL
                     TAGPGNATTSGPRVAFSFTVSDGAVACSLDGA"
     gene            complement(1271..>1628)
                     /locus_tag="VF516_08735"
     CDS             complement(1271..>1628)
                     /locus_tag="VF516_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107804.1"
                     /translation="PLHRTWTATRAISAVLGVAGAGALGTGIYFGVHAHDLANRADGR
                     CPGTVCNDPEGLRLNDQAKTAASRANVLYIAGGAALVSAAVLWWVGGPHEVAVVPVAA
                     SSPAGGRVGLAMTGRF"
BASE COUNT          195 a          590 c          629 g          214 t
ORIGIN      
        1 gcgccgtcga ggctgcacgc gaccgcgccg tcgctgaccg tgaagctgaa cgcgacccgc
       61 gggccgctcg tggtcgcgtt gcccgggccc gcggtgagcg cgacgtcggg cgccacggtg
      121 tcgaccgtcc acgaccgggt cgccggcgtc ggatcgtcgt gcccggcggc gtcggtcgcg
      181 cgcaccgcga acgtgtgcgc gccctgcgcg agcccggtca gcgcggccgg cgagctgcac
      241 gcggcgaacg gcgcgccgtc gaggctgcag ctgaacgtcg agtcgggctc gctcgagctg
      301 aacgacaggc tcgcgctggc gctcggcacc gtgccgctcg gccccgcggt gatcgcggtg
      361 tccggcggcg tgagatcgac cgtccagctg cgggtcgcgg gcgtcggatc gaagttgccc
      421 accgcgtcgc gtacccggac cgcgaagccg tgcgccgctt cccgcagccc gctgtaggtc
      481 tgcggcgagc tgcacgcggt gaacgcggcg ccgtcgagcg agcactggaa cgtcgcgccg
      541 gcgcccgcgt cgggcgacac gaaggtgaac gtcgcggtcg tgctcgggct cgccgcgggc
      601 gggccgccga cgatctgggt gtcgggcgtg cgggtgtcga ccgtccacgc gtagatcgcc
      661 ggcgacgcgt cgaggttgcc ggcgcgatcg tgcgcgcgca ccgcgaagct gtgcggcccg
      721 tcgccggcga gcgcgaacga ctggccgctc gtgcacggca ggtagccggc gttgtcgacc
      781 gagcagtcga agctgacgtt gtcctcgctg gaccggaacg tgaactgggc ggtgacgctg
      841 ttgtcggccg ccggcggccc ggtcaacagg atcgtgtccg gcgggacggt gtcgatcgtc
      901 cacactcgct cggccggcgt gtcgtcgctg ttgccggcgg ggtcgaccgc gcgcaccgag
      961 aagctgtgcg gcccgtcggg cagcgtccgc acgtacggcg actggcacgg ctgggcggcc
     1021 tcctgatcga tccggcactc gaacgtcgcg ctcgggtcgt cggacgagaa ccggaacgcc
     1081 gcctgaccgc tgttgctgaa cgcctccggc gcctgatcga tccgggtatc cggcgcgtgg
     1141 gtatcgaccg ggccgccgcc cgcgtcgccg gggccggcgt cgggctcgtc ggggtgcttg
     1201 tcggcgacct tgcagccgcc ggcgagagcc aggacggcca gggcgggcca cgagacgcga
     1261 gcggtgcgca tcagaacctc cctgtcatgg cgaggccgac gcggccgccg gccggcgagc
     1321 tcgctgcgac cgggaccacg gcgacctcat ggggcccgcc gacccaccac agcacggccg
     1381 cgctcacgag cgccgcgccg ccggcgatgt acagcacgtt ggcgcgcgac gccgcggtct
     1441 tcgcctgatc gttgagccgc aggccctctg gatcgttgca cacggtcccc ggacagcgcc
     1501 catcggcgcg attcgcgagg tcatgggcgt gcacgccgaa gtagatgccg gtgccgagcg
     1561 cccctgcccc ggcgacgccc agcaccgccg agatcgcccg ggtcgcggtc caggtccggt
     1621 gcagcggc
//
LOCUS       DASVOR010000439        10399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_559568,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000439 DASVOR010000000
VERSION     DASVOR010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559568"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..988)
                     /gene="cfa"
                     /locus_tag="VF516_08740"
     CDS             complement(<1..988)
                     /gene="cfa"
                     /locus_tag="VF516_08740"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEX8107805.1"
                     /translation="MKHEDFSQAEKIIRDLFGQAGIAIGGGAPHDIRVKDRRFYPRVL
                     ADGSLGLGEAYIDDWWDCDAIAEMIVQLIRAELHHKIRLSPRLVVESVKARVLNMQRP
                     SNAYHNIAHYDLGNELFEHMLGPTMAYSCGYWRDATDLDSAQRAKFDLICKKLMLQPG
                     ERLLDIGCGFGTLARHAAEHYGVRVVGITIAREQHAYAQKLCAGLPIDIRLLDYRELP
                     SLGRFDKIVSVGMFEHVGVKNYRRYLELAAAALPDGGLFLLHTIGTGVSRMTGDAYLN
                     KYIFPNGVLPSPTNLGRALEGVFVVEDWHNFGHDYFPTFMAWHANFQRYVASD"
     gene            1036..2139
                     /locus_tag="VF516_08745"
     CDS             1036..2139
                     /locus_tag="VF516_08745"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8107806.1"
                     /translation="MSDRELRIAIRRDLPAYLFRPRPWRALWAVPMVAVIVVGTWAVI
                     ALPLPLALAIPLSLVVGNTYASLLHFAHEVGHGATVRQRWLQDVILALGLAAYWVEPA
                     FWRAWHNRAHHGHTNVPDADPDSVGTVAAYRRAGAWHKRLLLALSPGPGHWLSALYLF
                     FTFNVHGQVVLWSKSRHQPGFERLRRGRAGLYSAAVASGYLALAVASGWRGAILGVLV
                     PTMIANLGVMAYIVTNHMLRPLSTARDSLTTSMSVTAHPILDWLHFHFSHHVEHHLFP
                     TMASDGLPRVRAWLQRRIGDRYFAPPFGGALVAIFRTPRLYADPVTLVDPERGTQRAL
                     ADVERELRGARPGEPPPGVCDGAPGPAAAIRSP"
     gene            complement(2126..2599)
                     /locus_tag="VF516_08750"
     CDS             complement(2126..2599)
                     /locus_tag="VF516_08750"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEX8107807.1"
                     /translation="MKTERLPMAPGCGGCSRRELLQGLGVAAVGALVVAAGCSQQGSS
                     LSTATTSSCGAGECIDLTNSANKELTAAGGAMLVDMAGDTIMVVRISDTQVVAMSAIC
                     THAGCSMDYNAGQKVLDCPCHGSQFATDGTVVRGPAVRPLRVYTVTLASNMITVI"
     gene            complement(2950..4617)
                     /locus_tag="VF516_08755"
     CDS             complement(2950..4617)
                     /locus_tag="VF516_08755"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8107808.1"
                     /translation="MATDSTAKPATSSPTTSLSAEERKVIFASSLGTVFEWYDFYLYA
                     TLAPFFAGLFFPKGNATAALLSALATYAAGFLVRPFGALLFGRIGDLVGRKYTFLVTI
                     TVMGISTFAVGLLPTYESLGVGAPLLLTLLRLLQGRARGGEYGGAAIYVAEHSHPQRR
                     GYSTSWIQTTATLGFFLSLLVIGLCRGLFAKEAFESWAWRIPFWASLVLLIFSIYIRL
                     RLYESPVFLRMKEEGRVSKRPLTESFLRYPNNKYVFLALFGAVAGQGVVWYTGQFYAL
                     FFLTGTLKVDFLTAYILIGIALLIGTPLFLVFGRLSDRIGRRKIILAGCLIAAVTYFP
                     VFKGLTHFTNPDLAESSERMKIRVAADDCQLHIFSFPGTEYTQCDRVKDFLTKAGLSF
                     ESDPAVPGKPVVTKIGETTIEGWQLEGGKEAPRTGGSPDDPRFKKALADNHYPAAAKK
                     ENINYVMVMLLLVFLIVLVTMVYGPIAALLVEMFPAKIRYTSLSLPYHLANGWIGGML
                     PLVATAMAAASGNIYYGLWYPVVVAVITVIVGGLFLHDPSPDFDIHA"
     gene            complement(4744..6393)
                     /locus_tag="VF516_08760"
     CDS             complement(4744..6393)
                     /locus_tag="VF516_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8107809.1"
                     /translation="MTTSEAAASTPLTAEIVSKAEERRVIFASSLGTVFEWYDFYLYA
                     VLAPFFSTLFFPSGNPTAALLSAFVTYAAGFLVRPFGAIVFGRIGDLVGRKYTFLITI
                     TVMGASTFLVGLLPTFKTVGWAAPTILTLLRLTQGLALGGEYGGAATYVAEHAPRDKR
                     GRSTSWIQTTATVGLLLALAVIWTCRKVMEKDSFASWGWRIPFILSLVLLVFSVYIRL
                     KLQESPVFRRMKEEGRGSKQPLKESFFRYPNNKLVLLALLGAVAGQGVVWYTGQFQAL
                     FFLSSTLKLDPLITYELIAASLVIGTPFFIVFGRLSDRIGRLKIILAGCLIAALTYIP
                     LFKGLTHYTNPALEAFQEQTKVTVATDPSSCEIHIFRGPWTKFSACDNAKDFLTTRGI
                     SYEQLPPMEGKPVVVTIGDAQIEGWAGDAKAGSEKAATEALKAKGYPSAANPDNVNYF
                     MVILLLTIMVIYVTMVYGPIAAYLVELFPTKIRYTSMSLPYHIGNGWFGGMLPLLFTA
                     LVAASGDIYRGLWYPIIVSLLTVLIGVIFLRETPKNFDIHA"
     gene            7018..7806
                     /locus_tag="VF516_08765"
     CDS             7018..7806
                     /locus_tag="VF516_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8107810.1"
                     /translation="MRFHFDEPVVGYMTRDVEIARVDTPVEQIARTLQTRGISGVPIL
                     DGRGHLVGVVTRTDLIQLGVLQTGRRPTGSAMTLPHRRASEVMTHGAVEVSSSTSLRH
                     AGRVMIDNDIHRVFVTEAGQLAGVICAVDLTAAVRDAKIETPVAQVMTSPIVTIDIHQ
                     PLSAALDMLDRFHVTGLIVTEDGQPVGMFTQANALASRDQPRSTPIEAAYDAAVICLP
                     AEMKLYRVAAHVADLRARRVVVCKAREPVGIVSATDFARVVALS"
     gene            8059..9882
                     /locus_tag="VF516_08770"
     CDS             8059..9882
                     /locus_tag="VF516_08770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8107811.1"
                     /translation="MQGSLVGLSEVRHHSGWRRWRELTADAPPFLGPEFFALAAPLSD
                     GTDPVVASAWDGETMVGALPLVRDHHGLRGLRCDYTPGYDYCGTPEGVDAIWQALRRD
                     PSWAELTLVRIPAASPLATRLPSLATADGCPSIIRREPAHLYFPLAGFEQQLAPKFRS
                     NLQRCERKAGGVELERIAVPDRAAFDDALAIEAMAWKGAAGTAIEADPRAEHLYRALA
                     RLVGRQGRGALYFLRAGGRRIATLVAVEDRRTLYALKIGYDPSAYNLSPGHLLVWKVA
                     ADAEARGLQDFDFVGRNDDWKRKWTDRGREQVTIVVYRNTPRGLAHYAMSVLVRPHLP
                     ETLRDGWRSPLPRTCQRADIIGAHTLVGRVRGRLDRGLGIKSGIQRLIKPPPPRDPLG
                     QPSAFAPGSWVRVKSADELRTTLDDKNRTRGLLFTDAQWETAGKVFRTAHHVRRLRDD
                     HGRFRPVSRTVLLDGVDCAGDGPTPTGCGRHCPMMYRDEWLEPASAPPRAPGHAHPVR
                     HARVRDLHEILAGLDLRGRRDGLTFMPEMRAYVGKRFAIANKLTTVFEYDQWIATRAP
                     IYILDGLHCSGAVLGDRGPCDRGCALMWHEDWLHVEPETRA"
     gene            complement(9896..>10399)
                     /locus_tag="VF516_08775"
     CDS             complement(9896..>10399)
                     /locus_tag="VF516_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107812.1"
                     /translation="PSRADRAGSPVAGPVSGSGAASGAPATPDDPAHNGPGYNMTGLR
                     PHQAANCPSSLPGATTRLANTPRGVDVTVTSSDPVTAHRIVELAQLHVRGRTAEVPRP
                     HDQHHGGPGWIGSCPVMVTDQTQVSMTPRPDGATLHVDARSPDRVAEVQMLIKARAVR
                     LPGYLSS"
BASE COUNT         1643 a         3564 c         3673 g         1519 t
ORIGIN      
        1 tgtcggacgc gacgtagcgc tggaagttgg cgtgccaggc catgaaggtc gggaagtagt
       61 cgtggccgaa attgtgccag tcctcgacga cgaacacgcc ctccagcgcg cggccgaggt
      121 tggtcggcga cggcaggacc ccgttgggga agatgtactt gttgaggtag gcgtcgccgg
      181 tcatccggct gaccccggtg ccgatggtgt ggagcaggaa caggccgccg tcgggcagcg
      241 ccgccgccgc cagctcgagg tagcgccggt agttcttgac cccgacgtgc tcgaacatgc
      301 cgaccgagac gatcttgtcg aaccggccga ggctgggcag ctcgcggtag tcgagcaggc
      361 ggatgtcgat cggcagcccg gcgcacagct tctgggcgta ggcgtgctgc tcgcgcgcga
      421 tcgtgatgcc gacgacccgg accccgtagt gctcggcggc gtggcgggcg agcgtgccga
      481 acccgcagcc gatgtcgagc aggcgctcgc cgggctggag catgagcttc ttgcagatca
      541 ggtcgaactt ggcgcgctgg gctgaatcga ggtcggtggc gtcgcgccag tagccgcacg
      601 agtacgccat ggtcggaccg agcatgtgct cgaacagctc gttgccgagg tcgtagtgcg
      661 cgatgttgtg gtaggcgttg gacggccgct gcatgttgag cacgcgggcc ttgacgctct
      721 cgaccaccag ccgcggcgac agccggatct tgtggtggag ctcggcgcgg atcagctgga
      781 cgatcatctc ggcgatcgcg tcgcagtccc accagtcgtc tatataagcc tcgccgaggc
      841 cgagcgagcc gtcggccagc acccgcgggt agaaccggcg gtccttgacc cggatgtcgt
      901 gcggggcccc cccgccgatc gcgatgccgg cctggccgaa caggtcgcgg atgatcttct
      961 ccgcctggct gaaatcctcg tgcttcacgc gcgcgaagag tacgccttgg gtatggtcta
     1021 taccagaaag gaagcatgtc cgaccgcgag ctcaggatcg cgatccgccg ggatctaccg
     1081 gcgtacttgt tccgcccgcg gccgtggcgt gcgctgtggg cggtgccgat ggttgccgtg
     1141 atcgtcgtcg gaacatgggc ggtgatcgcg ctgccgctgc cgctggcgct ggcgatcccg
     1201 ctgtcgctcg tggtcggcaa cacctacgcg agcctgctcc acttcgcgca cgaggtcggc
     1261 cacggcgcga cggtgcggca gcgctggctg caggacgtga tcctcgcgct cggcctcgcg
     1321 gcctactggg tcgagccggc gttctggcgc gcgtggcaca accgcgccca ccacggccac
     1381 accaacgtgc ccgacgccga ccccgactcg gtcggcacgg tcgccgccta ccggcgggcc
     1441 ggcgcctggc acaagcggct gctgctcgcg ctgtcgccgg ggcccggcca ctggctgagc
     1501 gcgctgtacc tgttcttcac gttcaacgtg cacggccagg tcgtgctgtg gagcaagtcc
     1561 cgccatcagc ccgggttcga gcggctgcgc cggggccgcg ccggcctgta cagcgcggcg
     1621 gtcgcgagcg gctacctcgc gctggcggtg gcgtcgggct ggcgcggcgc gatcctcggc
     1681 gtgctcgtac cgacgatgat cgccaacctc ggggtgatgg cgtacatcgt gaccaaccac
     1741 atgctgcgcc cgctgtcgac cgcgcgcgac agcctgacca cgtcgatgag cgtgaccgcg
     1801 cacccgatcc tcgactggct gcacttccac ttcagccacc acgtcgagca ccacctgttc
     1861 ccgacgatgg cgtcggatgg ccttccgcgg gtccgcgcgt ggctgcagcg gcggatcggc
     1921 gaccgctact tcgcaccgcc gttcggcggc gcgctggtcg cgatcttccg gacgccgcgg
     1981 ctgtacgccg atccggtcac gctggtcgac ccggagcgcg ggacgcagcg agcgctggcc
     2041 gacgtcgagc gcgagctgcg cggcgcgcgg cccggtgagc caccgccggg ggtgtgcgac
     2101 ggcgcccccg gcccggccgc cgcgatcaga tcaccgtgat catgttgctg gccagcgtca
     2161 cggtgtagac gcgcagcggg cggaccgcgg gaccgcgcac gaccgtgccg tcggtcgcga
     2221 actgcgagcc gtggcacggg cagtcgagca ccttctggcc ggcgttgtag tccatcgagc
     2281 acccggcgtg cgtgcagatc gccgacatcg cgacgacctg ggtgtccgag atccggacga
     2341 ccatgatcgt gtcgccggcc atgtcgacca gcatcgcgcc gcccgcggcg gtgagctcct
     2401 tgttcgcgga gttggtgaga tcgatgcact ctccggctcc gcagctggac gtggtcgcgg
     2461 tcgacagcga cgatccttgc tggctgcagc cggcggcgac gacgagtgcc ccgaccgccg
     2521 cgacgccgag gccctggagc aactcgcgcc gcgagcaacc gccgcatccc ggggccatcg
     2581 gcaagcgttc tgtcttcatc tgttgtgtac ctcgaagctg tgtggcccgt gagaccggga
     2641 tccttcgcct cgcgttgcac cgtcatgcac gtttgccgat gtgcatgaat tgcacgagac
     2701 gcatcgccgg tgacacgccc aacgatgaac gcaaggcatg cgccgcaagc gacggcgtcg
     2761 agcgcgacgg tactcacgcg gttagccgct tgccagcgag gcaatcgcgc ccgctgtgcg
     2821 atgcccgatg tgcggcgcgg ccggcggact gagtgccagt ccggcgcgcg tacatgtcgt
     2881 gcgcgacgcg ccggcgctcg accccaaggc gccggcgcgc aaacagcgcg ccagcgcgca
     2941 aacagcgcgt caggcgtgaa tgtcgaagtc tggcgacgga tcgtgcagga acaagccgcc
     3001 gacgatcacc gtgatgacgg cgaccacgac cggataccac agtccgtaat agatgttgcc
     3061 ggacgctgcc gccatcgccg tggcaaccag cggtaacatg ccgccgatcc agccgttggc
     3121 gaggtgatac ggcagcgaca gcgaggtgta ccggatcttg gcggggaaca tctcgacgag
     3181 cagcgccgcg atcggtccgt agaccatggt cacgagcacg atcagaaata ccaggagaag
     3241 catcaccatc acgtagttga tgttctcctt cttggcggca gccggataat gattgtcggc
     3301 gagcgctttc ttgaaccgcg ggtcatcggg gctgcccccg gtcctgggtg cctccttgcc
     3361 gccttcgagc tgccagccct cgatcgtggt ctcgccgatc ttggtgacca ccggcttgcc
     3421 ggggaccgca gggtcggact cgaacgacag cccggccttg gtcaggaagt ccttgacccg
     3481 gtcgcactgc gtgtactcgg tgcccgggaa cgagaagatg tgaagctggc agtcgtcggc
     3541 ggcaacccgg atcttcatcc tctcggagga ctcggcgaga tcggggttgg tgaagtgggt
     3601 cagccccttg aacaccggga agtaggtcac cgcggcgatc aggcagccgg cgaggatgat
     3661 cttgcggcgg ccgatccgat ccgacagcct gccgaacacc aggaacagcg gcgtgccgat
     3721 caacagcgcg atgccgatca ggatgtacgc ggtgaggaag tcgaccttga gcgtgccggt
     3781 caggaagaac agcgcgtaga actggccggt gtaccagacc acgccctggc ccgcgaccgc
     3841 gccgaacagc gcgaggaaga cgtacttgtt gttggggtag cggaggaagc tctcggtcag
     3901 cggccgcttg ctgacccggc cctcctcctt catgcgcagg aacaccggcg actcgtagag
     3961 ccggagccgg atgtagatcg agaagatcag gagcaccagc gaggcccaga acgggatccg
     4021 ccacgcccac gactcgaacg cctccttggc gaacagcccg cggcacaggc cgatgaccag
     4081 gagcgacagg aagaacccca gggtcgccgt ggtctggatc cagctcgtcg agtagccgcg
     4141 ccgctgcgga tgcgagtgct ccgcgacgta gatcgcggcg ccgccgtact cgccgccgcg
     4201 cgcgcggccc tgcagcaggc gcagcagggt gagcagcagc ggcgcgccga ccccgagcga
     4261 ctcgtacgtg ggcagcaggc cgaccgcgaa ggtcgatatg cccatcaccg tgatggtgac
     4321 gaggaacgtg tacttgcggc cgaccaggtc gccgatccgg ccgaacagca gcgcgccgaa
     4381 cggccgcacc aggaacccgg cggcgtaggt cgcgagcgcc gagagcagcg ccgcggtggc
     4441 gttgcccttg ggaaagaaca ggcccgcgaa gaacggcgcg agcgtcgcgt agagatagaa
     4501 gtcgtaccac tcgaacacgg tgcccagcga cgacgcgaag atgaccttgc gttcttcggc
     4561 ggagagcgac gtggtcggcg agctggtggc tggcttggcg gtggagtctg tggccatggc
     4621 tgtcctttgc tcaagacacg gaggtgcgcc tgcaggtccc ggtgacgtgg agccggcggg
     4681 ggatctgccg gcgagcgagc cggccgaggc cgaggacaga tgacgatccc cggccggacg
     4741 gcgctaggcg tggatgtcga agttcttggg cgtctcgcgc aggaagatca cgccgatcag
     4801 gacggtgagc agcgagacga tgatcgggta ccacaggccg cggtagatat cgcccgaggc
     4861 cgcgaccagg gcggtgaaca gcagcggcag catgccgccg aaccagccgt tgccgatgtg
     4921 gtacggcagt gacatcgatg tgtagcggat cttggtcggg aacagctcga ccaggtaggc
     4981 agcgatcggc ccgtatacca tggtcacgta gatcaccatg atggtgagca ggaggatcac
     5041 catgaagtag ttgacgttgt cgggattggc cgcgctggga tagcccttgg ccttcagcgc
     5101 ctcggtggcc gccttctcgc tgccggcctt ggcgtccccc gcccagcctt cgatctgggc
     5161 atcaccgatc gtgacgacca cgggcttgcc ctccatcggt gggagctgct cgtacgagat
     5221 gccgcgggtc gtcaagaagt ccttggcgtt gtcgcatgcg ctgaacttgg tccagggacc
     5281 gcggaagatg tggatctcgc agctcgatgg gtcggtggcg accgtcacct tggtctgttc
     5341 ctgaaacgcc tccagcgccg ggttggtgta gtgcgtcagc cccttgaaca gcgggatata
     5401 ggtgagcgcg gcgatcaggc agcccgccag gatgatcttg agacgcccga tccgatcgga
     5461 cagccgcccg aagacgatga agaacggcgt gccgatgacc agcgacgcgg caatcagctc
     5521 gtacgtgatc agcgggtcga gcttgagcgt gctgctgagg aagaacagcg cctggaactg
     5581 ccccgtgtac cagacgaccc cctggccggc gaccgcaccg agcagggcga gcaacacgag
     5641 cttgttgttc gggtagcgga agaagctctc cttcagcggt tgcttcgagc cgcggccctc
     5701 ttccttcatg cgccggaaca ccggcgactc ctgcagcttg aggcggatgt agaccgagaa
     5761 cacgagcagg accagcgaca ggatgaacgg gatgcgccag ccccagcttg cgaagctgtc
     5821 cttctccatg accttgcggc aggtccagat gacggcgagc gcgagcagca ggccgaccgt
     5881 cgccgtggtc tggatccagc tcgtggaccg gccgcgcttg tcgcggggcg cgtgctcggc
     5941 gacgtaggtc gccgcgccac cgtactcgcc gcccagcgcg aggccctggg tcaggcgcag
     6001 cagggtcagg atcgtcggcg ccgcccagcc gacggtcttg aacgtcggaa gcaggccgac
     6061 gaggaacgtc gacgcaccca tcacggtgat cgtgatcagg aacgtgtact tacggccgac
     6121 cagatcgccg atccggccga acacgatcgc cccgaacggg cggaccagga agccggccgc
     6181 gtacgtgacg aacgccgaca gcagggcggc cgtcgggttg cccgatggga agaacagcgt
     6241 cgaaaagaac ggcgccagga cggcgtagag gtagaagtcg taccactcga agaccgtgcc
     6301 gagtgacgaa gcgaagatga cccgccgctc ctcggctttc gagacgatct cggcagtcag
     6361 cggagtcgag gctgcagctt cggaagtagt catccagttg ctccttgcgg agtgacacag
     6421 agagttatgc cgtcaggaac caccaatccc cgacgacagg aatgcgcaga tccgcttcga
     6481 ccagtaaccg gcctggccat ggaatgcaac aatgatgttt gcaaacaccg ccgagatcgc
     6541 gcagggcgcc agcgagcttg ctcggcgatc gcgacgcggg acgcggcgtg cctgaacagg
     6601 tggtcggggc gcgggtcgcg cctcgcgcac cgcccggccg ggccgccggc ccacgccgcg
     6661 gcgatctcgt gaccaccagg tgacggccgt ggcgacgccg cgcgcgagcg ccgaccttcg
     6721 cggctctcag cttccgccgg ccgcgcaccc tcccccgtga ccgatatgta cacattgcgc
     6781 ctacattgcc tgccacctcg acgcgatgcg ccaggccctc accacctgtt cagggtcgct
     6841 cggcgactgg ttttctcgga accacatgcc cgcgcggatc ggcggtcgca gccgcaaatc
     6901 gttcgcgcga tccgaccacc ggcgcagggt ttgccggacg aacgccggcc gcctgcgcaa
     6961 cctcccgacg gaacggcaaa aaacgcaggc ggcacgggac tcgcagcgcg ccggatcatg
     7021 cgctttcatt tcgacgagcc ggtcgtcggc tacatgaccc gcgacgtgga gatcgcccgc
     7081 gtcgacacgc cggtcgagca gatcgcccgc acgctgcaga cgcgcgggat ctccggcgtg
     7141 ccgatcctcg acggccgcgg ccatctggtc ggcgtcgtga cccggaccga tctgatccag
     7201 ctcggcgtgc tgcagaccgg ccggcggccg accggcagcg cgatgacgct gccgcaccgg
     7261 cgcgcgagcg aggtgatgac ccacggcgcg gtcgaggtca gctcgtcgac ctcgctgcgc
     7321 cacgccggac gggtcatgat cgacaacgac atccaccgcg tgttcgtgac cgaggccggc
     7381 cagctcgctg gcgtgatctg cgcggtcgac ctcacggcgg cggtgcgcga cgccaagatc
     7441 gagacgccgg tcgcacaggt gatgacctcg ccgatcgtga cgatcgacat ccaccagccg
     7501 ctgagcgccg cgctcgacat gctcgaccgc ttccacgtca ccggcctgat cgtcaccgag
     7561 gacggccagc cggtcgggat gttcacgcag gccaacgcgc tggccagccg cgaccagccg
     7621 cggtcgacgc cgatcgaggc cgcgtacgac gccgcggtga tctgcctgcc cgccgagatg
     7681 aagctgtacc gggtcgcggc gcacgtcgcg gacctgcgcg cgcgccgcgt cgtggtctgc
     7741 aaggcgcgcg agccggtcgg gatcgtcagc gcgaccgact tcgcgcgcgt cgtcgcgctg
     7801 tcgtgagccg ccgggcgcag cgcgcgccgg cgcgcgcgca ccacgagcct cgggacaacc
     7861 acgagggctg cggcgtgcca cccggccaca ccggtccgcg tcgccacgtc agcgatcgag
     7921 gtgatcgtcg catcgcgcga cgcgccggtg accgatcggc gagccagcga tgcgagatgc
     7981 gagcgtgagg cgcatacgcg atcgccggta cgagcgtgga tgcccgtcgc ggcatgtcgc
     8041 ttgctgatat ctcgcagcat gcaaggttcc ctcgtcggtc tgtccgaggt ccgacaccat
     8101 tcggggtggc ggcgctggcg cgagctcacg gccgacgcgc cgccgttcct gggcccggag
     8161 ttcttcgcgc tcgccgcgcc gctgtccgac ggcaccgatc cggtcgtcgc ctcggcgtgg
     8221 gacggcgaga ccatggtcgg cgcgctgccg ctggtgcgcg accaccacgg cctgcgcggc
     8281 ctgcgctgcg actacacacc gggctacgac tactgcggca cccccgaagg cgtcgacgcg
     8341 atctggcagg cgctgcgccg cgatccgagc tgggccgagc tcacgctggt ccggatcccc
     8401 gcggcctcgc cgctcgcgac ccggctgccg tcgctggcga ccgccgacgg ctgcccgtcg
     8461 atcatccgcc gcgagccggc gcacctgtac ttcccgctcg ccgggttcga gcagcagctg
     8521 gcgccaaagt tccgcagcaa cctgcagcgc tgcgagcgca aggccggcgg cgtcgagctc
     8581 gagcggatcg cggtccccga ccgggcggcg ttcgacgacg cgctcgcgat cgaggcgatg
     8641 gcatggaagg gcgccgccgg caccgcgatc gaggccgacc cgcgcgccga gcacctgtac
     8701 cgcgcgctgg cccggctggt cggccggcaa ggacgcggcg cgctgtactt cctgcgcgcc
     8761 ggcggccgcc ggatcgccac gctggtcgcg gtcgaggatc gccgcacgct gtacgcgctc
     8821 aagatcggct acgacccgag cgcgtacaac ctgagcccgg gccacctgct ggtctggaag
     8881 gtcgccgccg acgccgaggc ccgcggcctg caggacttcg acttcgtcgg tcgcaacgac
     8941 gactggaagc gcaagtggac cgatcgcggg cgcgagcagg tcacgatcgt ggtgtatcgc
     9001 aacacgccgc gcggcctggc gcactacgcg atgtcggtgc tggtccggcc gcacctgccc
     9061 gagaccctgc gcgacggctg gcgcagcccg ctgccgcgca cctgccagcg cgctgacatc
     9121 atcggcgccc acacgctggt cggccgggtc cgcggccggc tcgaccgggg actcggcatc
     9181 aagtcgggca tccagcggct gatcaagcca ccgccgccgc gcgatccgct cggccagccg
     9241 tcggcgttcg cgccgggctc gtgggtccgg gtcaagtccg ccgacgagct gcgcacgacg
     9301 ctcgacgaca agaaccgcac ccggggcctc ttgttcaccg atgcgcagtg ggagaccgcc
     9361 ggcaaggtgt tccgcaccgc gcaccacgtc cgccggctgc gcgacgatca cggccggttc
     9421 cgcccggtgt cgcgcaccgt gctgctcgac ggcgtcgatt gcgccggcga cgggccgacg
     9481 ccgaccggct gcggccggca ctgcccgatg atgtaccgcg acgagtggct cgagccggcc
     9541 tccgccccgc cgcgcgcgcc cggccacgcc catccggtgc gccacgcccg ggtccgcgat
     9601 ctacacgaga tcctcgccgg gctcgacctg cgcggccggc gcgatggcct gaccttcatg
     9661 cccgagatgc gcgcgtacgt cggcaagcgg ttcgcgatcg cgaacaagct caccacggtg
     9721 ttcgagtacg accagtggat cgcgacccgc gcgccgatct acatcctcga cggcctgcac
     9781 tgcagcggcg cggtgctcgg cgaccgcggg ccgtgcgacc gcgggtgcgc gctgatgtgg
     9841 cacgaggact ggctgcacgt cgagcccgag acccgcgcat gacattcgag cgccgtcacg
     9901 acgacaggta gccgggcagg cggaccgcgc gggccttgat cagcatctgc acctcggcca
     9961 cgcgatccgg cgaccgggca tcgacgtgca gggtcgcgcc gtcgggccgc ggcgtcatcg
    10021 agacctgcgt ctggtcggtc accatcaccg ggcaggagcc gatccagccc ggaccgccgt
    10081 ggtgctggtc gtgcgggcgc ggcacctcgg cggtccggcc gcggacgtgg agctgggcga
    10141 gctcgacgat gcggtgcgcg gtcacgggat cgctcgacgt caccgtcacg tcgacgccgc
    10201 gcggcgtgtt ggcgagccgg gtggtcgcgc cgggcagcga cgacgggcag ttcgccgcct
    10261 ggtgcggccg caggccggtc atgttgtatc ccggcccgtt gtgggccggg tcgtcggggg
    10321 tcgccggcgc accggacgcg gcgcccgatc cgctgaccgg ccccgcgacc ggcgagcccg
    10381 cgcggtcagc gcgggatgg
//
LOCUS       DASVOR010000440         4565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_296470,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000440 DASVOR010000000
VERSION     DASVOR010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4565
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296470"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="VF516_08780"
     CDS             complement(<1..418)
                     /locus_tag="VF516_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107813.1"
                     /translation="MWAELHQALTADAAVIVHGATAGWSAWLAAQLPELGAGGSDHDV
                     VVVVAPDDAAARRLEADIRFFRGGAGDPAAALDDIAALPGIDVSPYADLSPDRSCIVE
                     RVATLYRLTQPALRPRIVVSSAEALVRRTVAPGELAA"
     gene            complement(483..929)
                     /locus_tag="VF516_08785"
     CDS             complement(483..929)
                     /locus_tag="VF516_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107814.1"
                     /translation="MRAIPGGDRNYIGGALRPTFLDSLHLDEATRPQHPGDARWDYLL
                     GHDGTSQIIAVEVHSANTSNAAEVVRKRVASGDHLKRHLQPGETVAAWYWVASGHVDF
                     VPYERTIRYLDQNGIQFVGSRLETKHLASLSRRAVAPAHRRKKPRK"
     gene            complement(1004..2167)
                     /locus_tag="VF516_08790"
     CDS             complement(1004..2167)
                     /locus_tag="VF516_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8107815.1"
                     /translation="MSSDRLVIRDFAQIRKARIQFRDLTVLVGSQGTGKSLVLQWLKV
                     AIDGKYIVQMLRDAGQDVTQKTVIDQIFGRGMRSAWRETTEINFDGADLSPATIEKCG
                     DGVERLFFIPAHRSMLISDGWATSFQKLTSETPVVARLFSQNLLDRFNAREGARLFPL
                     DRLLKKVYRELINDAVFHGGTVGVRETEQQTKRLRLRFKGEQKMSLPFMTWTAGQREF
                     TPLLLGLYHLLPQRKQTKDDRIDWIVIEEPEMGLHPEAVTVVMLLVLELLWRGYRVVL
                     STHSPVVVTAVWALQRLAATQASHQLVCDAFSVPAKYRVSLRSVATEALQKKFATYVM
                     KYKRGQATAIDISSLDPGAEDDAVSGWGGLAGFSSRFGESVRKAVNSAPGRAA"
     gene            complement(2260..2976)
                     /locus_tag="VF516_08795"
     CDS             complement(2260..2976)
                     /locus_tag="VF516_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107816.1"
                     /translation="MTVALVAIAGLGAATCAGTMAEQPAPKENSDVAHAATPPALSPP
                     LEAELAAIRRDPLAAVGFGEKAYLPYELVKGRELVDSPEPGVVPRLSTEALDAHNDRV
                     FRLVMLQILGLRREAAVDTTLVSALADPAVRPLAAYLLGRPGFKGYPARDRKSVAPLL
                     RALATHLDDAGHYDDPWYQRAYATGDLVLAAFVRLAGVDRFVFANPEDRDFLGYTLHF
                     PDAERAGLRAQAKQMPIPAE"
     gene            complement(2973..4328)
                     /locus_tag="VF516_08800"
     CDS             complement(2973..4328)
                     /locus_tag="VF516_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8107817.1"
                     /translation="MDRLYRGGGSNEGATSERATTGTPGKRTLTEALVQRKPDGAGAA
                     AATGEPPPAIAALIQRTNGDPAKLRAAVLADPPLRYDLAAYLASGGDPALNELLAKAF
                     PPRIASTAAQTPDATTPEHEKDPKDATVPLPAARPDTKTLAKGVMTWKLQAITHSDAR
                     VDVDFLPDKTKVDAKNVSFVQTVLNTLNGNPKYAGATKSNPVGNKTLYSPYEEAKEKR
                     RVDHGASSENDPFYGAEWDNSAKQWKNEGGGTVVGKSTKGSSSSSAKMNDNPGTGLGR
                     EGKGDTVMEFETVPVVLETREPLGALKWGYKIQDKDNAPIELTGATKADCTDSPSATW
                     GSALDKFYEAKFEILDGFAQDKADLTSGHTAILDGIVTKMKAKPALRAELGGAADLKD
                     ADPATISQARADAAKNYLVSKGIDAGRIDTQSYGADWARVATTAGAAEPKNRRVQIWV
                     K"
BASE COUNT          676 a         1564 c         1514 g          811 t
ORIGIN      
        1 gcgccgcgag ctcgccgggc gccaccgtgc gccggaccag cgcctcggcc gagctcacca
       61 cgatccgcgg ccgcagggcg ggctgggtca gccggtacag cgtcgcgact cgctcgacga
      121 tgcagctccg gtccggcgac aggtcggcgt acggcgacac gtcgatcccg ggcagcgccg
      181 cgatgtcatc gagcgccgcc gccgggtcgc cggcgccgcc gcgaaagaac cggatgtcgg
      241 cctcgagccg gcgcgccgcg gcatcgtccg gcgcgaccac cacaaccacg tcatgatcac
      301 tgccgccggc gccgagctcg ggcagctgcg ccgcgagcca cgccgaccac ccggcggtcg
      361 cgccgtgcac gatcaccgca gcatccgcgg tcagcgcctg gtgtagctcg gcccacatcg
      421 gccgcgcacc ctagcatgcg gatccccggt gcgaagtgca ccacatgcat acgcatcgcg
      481 tgctacttgc gcggcttctt tctgcgatgg gccggcgcta ccgcccgcct ggatagcgat
      541 gccaggtgct tcgtctcgag gcgacttccc acgaactgga tgccgttctg atccaggtag
      601 cgaattgtcc gctcatacgg aacgaagtcc acgtggccgg atgccaccca gtaccatgcc
      661 gcgacggtct ccccaggctg caggtgacgt ttcaggtgat cgccggaggc cacgcgtttc
      721 cgcaccacct ccgccgcgtt cgatgtgttt gcggagtgta cctcgaccgc gatgatctga
      781 gacgtgccgt catggcccag cagatagtcc cagcgggcgt cgcccggatg ctgcggtcgg
      841 gtagcctcgt cgaggtggag gctatccagg aacgttgggc gcagtgctcc cccgatgtag
      901 tttcggtcac cgcccgggat ggctcgcaaa ccgggccgga tcaatctcgc aagactgctg
      961 ccccggagcg cccgcgctac tggaccgctt gctactggac cgctcatgct gcccttcccg
     1021 gggcgctgtt caccgccttt cgaaccgatt ccccgaaccg ggagctgaaa ccggccagcc
     1081 ctccccatcc agatacggcg tcgtcctctg caccgggatc gaggctggag atgtcgatcg
     1141 cggtcgcctg accgcgcttg tacttcatga cgtacgtggc aaacttcttc tggagtgcct
     1201 cggtggctac cgaccgaagg ctgactcggt acttcgctgg gacgctgaac gcatcacaga
     1261 cgagctggtg cgaggcttgg gtcgccgcga gacgttgcag cgcccagacc gccgtgacca
     1321 cgaccggcga gtgggtcgac agcacgaccc gatacccgcg ccatagcagc tcgaggacga
     1381 gcagcatcac cacggtcacc gcttctgggt gaagccccat ctcgggctcc tcgatgacga
     1441 tccagtcgat gcggtcgtcc ttcgtctgct ttctctgcgg cagcaagtgg tagagcccga
     1501 gcaggagcgg cgtgaactct cgttggcctg cggtccatgt catgaagggg agtgacatct
     1561 tctgctcccc cttgaagcga agccgcagcc gcttggtttg ctgttcggtc tccctgacac
     1621 ccactgtgcc gccgtggaac acggcatcat tgatgagctc gcggtagact ttcttcaata
     1681 gccgatcgag aggaaacaga cgtgcgccct cacgcgcgtt gaagcgatcg agcaggttct
     1741 ggctgaacag gcgggccacc accggcgtct cggacgtcag cttctgaaac gatgtcgccc
     1801 atccgtcgct gatgagcatc gagcggtgtg cgggaatgaa gaacagtctt tcgacgccgt
     1861 caccgcactt ctcgatcgtt gcgggtgaca gatccgctcc gtcgaagttg atctccgtgg
     1921 tttcgcgcca agcggaccgc atgcccctgc cgaagatctg atcgatcacg gtcttctggg
     1981 tgacgtcctg acccgcgtcg cgcagcatct gaacgatgta ctttccgtcg atggcgacct
     2041 tgagccactg gagtaccaag ctcttgccgg tgccttgcga gccgaccagg accgttagat
     2101 cacgaaactg gatccgcgcc ttcctgatct gcgcgaagtc gcgaatgacg agacggtcgg
     2161 acgacatacg cccacgacct tacatcatgg cttccttgcg gcgtacctcg gcgcggcaag
     2221 cttcacggcg caatccggga gggcagacat ggtcggtggc tactccgccg ggatcggcat
     2281 ctgcttcgcc tgcgcccgca acccggcgcg ctcggcgtcg gggaagtgca gcgtgtaacc
     2341 gaggaaatcg cggtcctcgg ggttcgcgaa cacgaatcgg tcgactccgg cgagccggac
     2401 gaacgcggcc agcacgaggt cgccggtcgc gtaggcgcgc tgataccagg gatcgtcgta
     2461 gtggcccgcg tcgtcgaggt gtgtcgccag cgcgcgaagc agcggcgcga cggacttgcg
     2521 gtcgcgcgct gggtagccct tgaagccggg ccggcccagc aagtaggccg caagcggccg
     2581 caccgcggga tcggcgaggg ccgagaccag cgtcgtgtcc accgccgcct cgcggcgcag
     2641 cccgaggatc tgcagcatca cgagccggaa cacccggtcg ttgtgggcat cgagcgcctc
     2701 ggtggacagg cgtggcacaa ccccgggctc gggactgtcg accagctcgc ggcccttgac
     2761 cagctcgtac ggcaggtacg ccttctcccc gaacccgacc gcggccagcg ggtcgcgccg
     2821 gatcgcggcc agctctgcct ccagcggagg cgacagcgca ggtggtgtcg ccgcatgtgc
     2881 cacgtcggaa ttctccttcg gagcgggttg ctccgccatc gtcccggcac aggtcgcggc
     2941 gccgagcccg gcgatcgcga ccagcgccac cgtcacttga cccatatctg tacccggcga
     3001 ttcttgggct ccgccgcacc cgccgtggtc gcgacccggg cccagtcggc gccgtaggac
     3061 tgcgtgtcga tccgcccggc gtcgatgccc ttcgagacca ggtagttctt cgccgcgtcg
     3121 gcgcgcgcct gcgagatcgt cgccgggtcg gcatccttga gatccgcggc cccgccgagc
     3181 tcggcgcgaa gcgctggctt ggccttcatc ttggtaacga taccatcgag gatcgcggtg
     3241 tgcccggacg tcaggtcggc cttgtcctgg gcgaatccgt cgaggatctc gaacttcgcc
     3301 tcgtagaact tgtcgagcgc gctgccccag gtggccgacg ggctgtccgt gcagtcggcc
     3361 ttggttgcgc cggtcagctc gatcggcgcg ttatccttgt cctggatctt gtacccccac
     3421 ttcagcgcgc ccagcggctc gcgggtctcg agaacgaccg gcacggtctc gaactccatg
     3481 accgtgtcgc ccttgccctc gcgcccgagc cccgtgcccg ggttgtcgtt catcttcgcc
     3541 gagctggacg agctgccctt cgtcgacttg ccgaccacgg tgccgccgcc ctcgttcttc
     3601 cattgcttcg cggagttgtc ccactcggcg ccgtagaacg ggtcgttctc cgacgacgcg
     3661 ccgtggtcga cgcggcgctt ctccttggcc tcctcgtacg gcgagtacag cgtcttgttg
     3721 ccgacagggt tcgacttggt ggcgccggcg tacttggggt tgccgttgag cgtgttgagc
     3781 acggtctgca cgaagctcac gttcttcgcg tcgaccttgg tcttgtcggg caggaagtcg
     3841 acatcgacac gcgcatcgga gtgggtgatc gcttgcagct tccaggtcat cacgcccttg
     3901 gccagcgtct tggtatcggg gcgtgctgcc ggtagcggga ccgtggcgtc cttgggatcc
     3961 ttctcatgct ccggcgtggt cgcgtccggc gtctgggctg cggtggacgc gatgcgcggc
     4021 gggaacgcct tggcgagcag ctcgttcagc gccggatcac cgccggaagc gaggtacgcc
     4081 gcgaggtcgt aacggagcgg cggatcggcg agcacggccg cgcgcagttt cgcgggatct
     4141 ccgttggtac gctggatcag cgcagcgatc gccggcggcg gttcgccggt agcggcggcg
     4201 gccccggctc catccggctt gcgctggacc agggcctcgg tcagcgtgcg cttcccaggg
     4261 gtgcccgtcg tggcacgctc gctcgttgcg ccctcgttcg atccacctcc acgatacaag
     4321 cggtccatga ggcccctccg cggcatggta cgcccgccgg gaggggttct tcccagtcgg
     4381 gccaagcatg tcggcggcgc ctcaccgcga gactcgtacg gtccgctgcc acgcgagggt
     4441 caagtgcaac aaagtcttac atgggcgtag gatgcgccgc atgtcgaacg cgatcgatcg
     4501 acgccagctg ctcggcgggc tggccgtgct cgcggcggtt cccgggtgcg cggcgtcggg
     4561 ctcgg
//
LOCUS       DASVOR010000441         1996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208693,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000441 DASVOR010000000
VERSION     DASVOR010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208693"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF516_08805"
     CDS             <1..272
                     /locus_tag="VF516_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107818.1"
                     /translation="SACPGEIRDELIDLESVASMLVVTLRRDRPARRRPELDHKVAAE
                     RERSGRLSGSPPTRFRQMASAADDYLNASVTHTRSCDSTPASRCW"
     gene            468..881
                     /locus_tag="VF516_08810"
     CDS             468..881
                     /locus_tag="VF516_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107819.1"
                     /translation="MGEQRVVVERPCPDCGAPARVIIGARFAEGDLRYFESLQCPSCR
                     CAQEADGHELDDYIRGALCAVHGRWSAHIRDLGPNRLGALFALRSHLRLTPVAALRVA
                     REARPIVNGALVEIEHVQAILEPAGIQLAIVRLPD"
BASE COUNT          312 a          708 c          695 g          281 t
ORIGIN      
        1 cgtcggcctg cccaggcgag atccgcgacg agctcatcga tctcgagagc gtcgcgtcca
       61 tgcttgtcgt gacgctgcga cgtgaccggc ccgcgcgccg tcgacctgag ctcgaccaca
      121 aggtcgccgc agaacgcgaa cggtcgggcc ggctctccgg atcccctccc accaggtttc
      181 ggcagatggc gagcgccgct gatgactacc tcaacgcgtc ggtgacccat acgcgctcct
      241 gcgactctac gcctgcctca cgatgctggt gacgcggctg cgaggcacgc gcgcggagcg
      301 gctcgcccgc gagaccggag acatcgacgc cgccgtcgcc agccggtgct tgcgcgacgc
      361 tccgcgcctg tcttaccgcg ccccgcgcgc cgcgacgtca cggcacgttc ttctacgcgt
      421 cgccgtccaa cgctgacaga ggtgtcgcca tgacgtatga atagccgatg ggggagcaga
      481 gggttgtcgt cgagagacca tgtcccgatt gcggcgcgcc tgcgcgggtc atcatcggcg
      541 ctcggttcgc ggaaggcgat ctgcgctact tcgagagcct ccagtgcccc agttgccggt
      601 gcgcacagga agccgacggc catgagctcg atgattacat ccgcggcgcg ctatgcgccg
      661 tgcacggccg ctggtcggcc cacatccgcg acctcggccc caaccgcctt ggcgccctct
      721 tcgccctgcg cagccacctc cgcctcacgc ccgtcgcggc cctgcgcgtc gcgcgcgaag
      781 cgaggcccat cgtcaatggc gcgctcgtcg agatcgagca cgtccaagct atcctcgagc
      841 cggccggcat ccagcttgcc atcgtccgtc tgccggactg accgctgcgc ggcgacgtca
      901 gagtcggtgg cacggacggg tcgggccggc tctccggatc ccttgttcta cgtgcagccg
      961 actgcgatca ccggtgctga ccacctcaag gcatcacctc aacgcgtccc ggaagtggat
     1021 ccatatccgg ccgcttcggt cacgtacagc caaccgcacc acggctcgga tccggccccg
     1081 cagtggatcc ctgctatccc gccgtcaaag ctcgtgaccg aggctgatcg gtggaagctg
     1141 gccgagcccg gtgcaggaag cgcggcaaga accggcaccc acgtgagggc gagcgggtcg
     1201 gcgaccggtc tggccggccg gcacttgctc gtgaccgagg ctggatcggt gaaggctgag
     1261 gctgagtcgc gtgggaagcg cggcaagaac cgcacccacg tgaggcgaac gagtttgcga
     1321 ccggagagcg cggcgttcac gtgagcgagc tggatcggtg gcagataagg tcgagccgga
     1381 ggggggacgc gtggcaagaa ccggcaccca cgtgggggcg agcggatcgc gaccagtctg
     1441 gccggccggc gcttccgcgt gaccgaggct ggatcggtga aagctcaggc cgagcccggc
     1501 gtaggaagcg cggcaagaac cggcacccac gagcccggtg caggaagcgc ggcaagaacc
     1561 cggcacccac ggtggggggg gcgagcgggt ccgcgaccag tctggccggc cggcgcttcc
     1621 gcgtgaccga ggctggatcg gtgaaggctg aggctgagtc gcatgggaag cgcggcaaga
     1681 accggcaccc acgtgggggc gagcgggcct gcgaccggtc taaccggccg gcgcttccgc
     1741 gtgaccgccc atccagtgca gccaagctcc ggcagcttcg gcgaagcagc gggcccgcca
     1801 tcgacctggt gcgcgggagt cgcaggtcca gggcttggcc taccgccgcg attggctcgc
     1861 cggcccgcct gtcacacctc cgagctacgc tccccccgtg gtcgaggccc agctcgatct
     1921 gttcggcgat cgccacctcc agctggaggg tgcacgccgc gcgctcaccg agggccgcgc
     1981 cgcggaggca tcgcgc
//
LOCUS       DASVOR010000442         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_296475,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000442 DASVOR010000000
VERSION     DASVOR010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296475"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VF516_08815"
     CDS             <1..959
                     /locus_tag="VF516_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107820.1"
                     /translation="VGDAVLVQRPVTEGWVHFLGMDKLVRDGRPQVWIPAGALHAWER
                     TVIAIHGDEITLDIPISDSIDQRQVSPPGATVVKASYPGRITHVGLEDFRIVSPPRAK
                     GSEFWFTRIEAVEDGWVRGVAVHDFTNGIWLGRGVKRFTIEDTHVTHDPTPYATSEAP
                     FDFSIDGQQTLVDRSSSQGGNKIWYYATQNAARGPNVVLNFRATGTASHVSSHDRWAT
                     GLLIDGSSVDGGIKLANNGHLGDGQGWTMGWGVVWNSGSDVRVEAPPGATNWAIGVTG
                     SVAIGAGRGIYDSLNRPVVPRSLYLAQLCLRRGPGAVAALGY"
     gene            complement(860..1441)
                     /locus_tag="VF516_08820"
     CDS             complement(860..1441)
                     /locus_tag="VF516_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107821.1"
                     /translation="MTWTATMPTINLVRAEIIDPQMSVIVPPTTINPGAMFDGQNPRA
                     GYAASADRYLFAWSFKTSTADELWVSLPDNQLTELHAVRLSTHGVLPRVAAGQDDFLV
                     AWKDTHMPSGIAAARVRLKCGSRSSSRPRGTDGDFVMLMTDRDAPGRPRERRAPHVRR
                     AQYPSAATAPGPRRRQSCARYRLLGTTGRFSES"
     gene            1661..2164
                     /locus_tag="VF516_08825"
     CDS             1661..2164
                     /locus_tag="VF516_08825"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEX8107822.1"
                     /translation="MTMRSPIGALCLCAEDDQLVGLYLPDRPAPSAQRGRGGVLARAA
                     AQLTEYFAGERRDFDLPLAPSGTEFQRTVWHALAEIPFGTTCSYGDLARVVGRPSASR
                     AVGAANGKNPIAILLPCHRVIGASGELTGYGGGLPMKRWLLDHERSCVQPPLPGVGDR
                     PAARGAR"
BASE COUNT          327 a          772 c          834 g          320 t
ORIGIN      
        1 cggtcggcga cgcggtgctg gtccagcgcc cggtgaccga ggggtgggtg cacttcctgg
       61 gaatggacaa gctggtgcgc gacggaagac cgcaggtctg gatcccggcc ggggcgctcc
      121 acgcgtggga gcgaacggtg atcgcgatcc acggcgacga gatcacgctc gacatcccga
      181 tctcggactc gatcgaccag cgccaggtca gcccgccggg ggcgaccgtc gtgaaggcga
      241 gctatccggg gcggatcacg cacgtgggac tggaggactt ccggatcgtc tcgccgcctc
      301 gcgcgaaggg cagcgagttc tggttcaccc gcatcgaggc ggtggaggac ggctgggtgc
      361 gcggcgtcgc cgtgcacgac ttcaccaacg ggatctggct gggccgcggc gtcaagcggt
      421 tcacgatcga ggacacgcac gtgacccacg atccgacgcc gtacgcgacg agcgaggcgc
      481 cgttcgactt ctcgatcgat ggtcagcaga cgctggtcga tcgcagctcg tcgcagggcg
      541 gcaacaagat ctggtactac gcgacgcaga acgcggcgcg cgggcccaac gtggtgctga
      601 acttccgggc caccgggacg gcgtcgcacg tctcgagtca cgatcgctgg gcgaccgggc
      661 tgctgatcga tggctcgtcg gtcgacggtg gcatcaagct cgccaacaac ggccacctcg
      721 gcgacggcca gggctggacg atggggtggg gcgtggtgtg gaactcgggc agcgacgtgc
      781 gggtcgaggc tccgccgggc gcgaccaact gggcgatcgg cgtcaccggc agcgtggcga
      841 tcggcgccgg gcgcggcatc tacgactcgc tgaaccggcc ggtggtgccc aggagcctgt
      901 acctcgcgca gctctgcctg cggcgcggtc ccggcgcggt cgccgcactc gggtactgag
      961 cgcgccgtac gtgcggcgca cgtcgctcac gtggtctgcc gggtgcatct cgatcggtca
     1021 tgagcatcac gaaatcaccg tccgtgccgc gcggcctcga gctgctgcgc gacccgcatt
     1081 tcaaccggac ccgcgccgcg gcgatccccg agggcatgtg ggtgtccttc caggcgacca
     1141 ggaagtcgtc ctggccggcg gcgacgcgcg gcagcacgcc gtgggtcgac agccggaccg
     1201 cgtgcagctc cgtcagctgg ttgtcgggca ggctgaccca cagctcgtcc gccgtcgagg
     1261 tcttgaacga ccacgcgaac aggtagcgat cggcggacgc ggcgtagccg gcgcgcgggt
     1321 tctggccgtc gaacatcgcg cccgggttga tcgtcgtcgg cggcacgatc accgacatct
     1381 gcgggtcgat gatctcggcg cgcaccaggt tgatcgtcgg catggtggcg gtccaggtca
     1441 cgaggaagcg acgagcgtga ggcccccgcg gaccacagaa tctgacgagg gtgatgtccg
     1501 gacgcagcga gtgcggcgcg atgcggcgtg atacgcagcg cacgatgaca cgtgcgcgac
     1561 acaccaccgg cgcgggctgc aggacattcg cgcgatactc cccacctgaa catgccgcgc
     1621 ggcgtgtcgc cgatcacgca ggagacccat gctgaccacg atgacgatgc gctcgccgat
     1681 cggagcgctg tgcctgtgcg ccgaggacga tcagctcgtc ggcctgtacc tgcccgaccg
     1741 gcctgcgccg tccgcgcagc gcgggcgggg cggtgtgctg gcgcgcgcgg ccgcccagct
     1801 caccgagtac tttgccggcg agcgccgcga cttcgatctc ccgctggcgc cgagcggcac
     1861 cgagttccag cgcaccgtgt ggcacgcgct ggccgagatc ccgttcggca cgacgtgcag
     1921 ctacggcgat ctcgcgcgcg tggtcggccg gccgtcggcg agccgggcgg tcggcgccgc
     1981 caacggcaag aacccgatcg ccatcctctt gccctgccac cgcgtgatcg gcgcgtccgg
     2041 cgagctcacc ggctacggcg gcggcctgcc gatgaagcgc tggctgctcg accacgagcg
     2101 cagctgcgtc cagccgccct tgcccggggt cggcgatcgg ccggccgcgc gcggcgcccg
     2161 atgagtgcta cggtcgcggc gtggactggt atctcctcgg cggactggcg gccggcgccg
     2221 tgtacctcgc gagccgcggc tcgctgcgca ggc
//
LOCUS       DASVOR010000443         3875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412647,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000443 DASVOR010000000
VERSION     DASVOR010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412647"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="VF516_08830"
     CDS             <1..180
                     /locus_tag="VF516_08830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107823.1"
                     /translation="GATLAWIAEARRSGVPRSNAAVVIGGRVYTGLSDPTLIQELVEA
                     ELAPGVLGSLPRWHR"
     gene            254..1558
                     /locus_tag="VF516_08835"
     CDS             254..1558
                     /locus_tag="VF516_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX8107824.1"
                     /translation="MKVELTFAAATDTGRQRTHNEDNFLIDKKLRLFLVADGMGGHAA
                     GEVASSIAVHEIRDVVHNSRDLIDRYRVDHPGVQGYEILQVLEHAIQAACSTVHARAQ
                     AEPDKRGMGTTATVLLIAGGGDHLRGFIAHVGDSRVYLARQNQCHVLTEDHSLMNELV
                     RRGKLNREQIESSPYKQYKNAVTRAVGVYGSVEVDTFDFDILPGDRFLLCSDGMYAYV
                     DEDELPKQLADGEVKDVPRKLVELANAGGGHDNITAVVVRIGETSSAELAPRTTEVSL
                     KLEVLKGMQMFRYMSYKELVRISNIAEMIELSTDQVIFSTGQPGDAMYVVLSGTVRLT
                     KGDTAVAELTRGHHFGEMALIDRSVRSLTARATEGTRLVVIHRKDFYDIIKREPALAT
                     KMLWSFVQVLGARLRKTTNDLSDALHGDRHGQETEAENLFQD"
     gene            complement(1577..2293)
                     /locus_tag="VF516_08840"
     CDS             complement(1577..2293)
                     /locus_tag="VF516_08840"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8107825.1"
                     /translation="MTRSYQESWDGYWRAVTPRPGVAFWDSTPDRAVQRELPVFASAF
                     DRALPVIDVGCGNGTQTRCLADAFSRVIGVDVSEEALRGARQYHGAANLEYRALDLLD
                     GAAIRALHAELGDANLYLRGVLHQMRAADRTPAVDHLAALLGERGLAFLVELSPAAGQ
                     LFAALAQRFGGPPGKLQYALSHGIAPASLQAGEVPALFHHAGCALRAQGTTTVVTTEA
                     LPGGEVIEVPCDYFLFQRAA"
     gene            2371..3165
                     /locus_tag="VF516_08845"
     CDS             2371..3165
                     /locus_tag="VF516_08845"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEX8107826.1"
                     /translation="MRRTIRLTVGLALGLALAACGHAASSPSSPGAPGPRIVAMISAN
                     AEWKIVRAQLPEAPHHDTPFGEWLVHRVGGQDVVFFHGGYGKVAAAGSTQYAIDRWHP
                     VLLVNLGTCGGFGDGRKVGDIVLASETTIYDIVELMGDPDEAIADFRTRLDTSTWPAR
                     LAGRVVIGPIVSADRDLDPAAVAGLAAKYHTGVGDWESGAIAWVASHNHTPVLILRGV
                     TDLVDAPGGDPTYNAVDVWQRASIAVMASLVALLGDALPDVLPSLR"
     gene            complement(3184..>3875)
                     /locus_tag="VF516_08850"
     CDS             complement(3184..>3875)
                     /locus_tag="VF516_08850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX8107827.1"
                     /translation="GFAVSPIRVLASAMWLVGFAGCYNPSALPEGAPCQRSEQCPEPQ
                     QCVLGSCSRSAPPPVDAHPTPPPDAQMIDAMVDAMPVPCDADGLTCGGTPTVFLCGTQ
                     CWVKCTDLVSRATAETRCTGWTGALGEIDDATENGCVTMKISTATFWFGAIQGAGAMT
                     PGDKWTWNGDATKLFNSTRYINWGTNKPDDADGNEDGAEQCGTIRPGGTWDDEGCNGS
                     LGFFCRRSTFR"
BASE COUNT          583 a         1428 c         1307 g          557 t
ORIGIN      
        1 ggcgcgaccc tggcctggat cgccgaggcc cggaggtccg gggtgccgcg cagcaacgca
       61 gcggtcgtga tcggcggccg ggtctacacc gggctgtccg acccgaccct gatccaggag
      121 ctggtcgagg ccgagctggc gcccggggtg ctcggctccc tcccccgctg gcatcgctag
      181 cccggcgggc gagcccgccc cggaatggtt gccgtgacgc tggtttgacc gtcggacccg
      241 cgcgtgttac ccgatgaagg tggagctgac gttcgcggca gcgaccgaca cgggtcggca
      301 gcgcacgcac aacgaggaca acttcctcat cgacaagaag ctccgtctgt tcctggtcgc
      361 cgacggcatg ggcggccatg ccgccggcga ggtggcgtcg tcgatcgcgg tccacgagat
      421 ccgcgacgtc gtccacaaca gccgcgatct gatcgaccgc taccgcgtcg atcaccccgg
      481 cgtccagggc tacgagatcc tgcaggtgct cgagcacgcg atccaggccg cgtgcagcac
      541 cgtgcacgcc cgcgcccagg ccgagcccga caagcgcggc atgggcacca ccgccaccgt
      601 gctgctgatc gccggcggcg gcgaccacct gcgcggcttc atcgcccacg tcggcgacag
      661 ccgggtctat ctcgcgcgcc agaaccagtg ccacgtgctg accgaggacc actcgctgat
      721 gaacgagctg gtccgccgcg gcaagctcaa ccgcgagcag atcgagagct ccccctacaa
      781 gcagtacaag aacgcggtga cccgcgccgt gggggtatac ggctcggtcg aggtcgacac
      841 cttcgacttc gacatcctgc ccggcgaccg cttcctcctg tgctcggacg gcatgtacgc
      901 ctacgtcgac gaggacgagc tccccaagca gctcgccgac ggcgaggtca aggacgtgcc
      961 gcgcaagctc gtcgagctcg ccaacgccgg cggcggccac gacaacatca ccgccgtcgt
     1021 ggtccggatc ggcgagacct cgtcggccga gctcgcgccg cgcaccaccg aggtctcgct
     1081 caagctcgag gtcctcaagg gcatgcagat gttccgctac atgtcctaca aggagctggt
     1141 ccggatctcc aacatcgccg agatgatcga gctgtcgacc gaccaggtga tcttctccac
     1201 cggccagccc ggcgacgcga tgtacgtcgt gctgtcgggc accgtgcggc tcaccaaggg
     1261 cgataccgcg gtcgccgagc tgacccgcgg ccaccacttc ggcgagatgg cgctgatcga
     1321 tcgctcggtg cgctcgctga ccgcgcgggc caccgagggc acccggctgg tcgtcatcca
     1381 ccgcaaggac ttctacgaca tcatcaagcg cgagcccgcg ctcgccacca agatgctgtg
     1441 gagcttcgtc caggtgctcg gcgcccggct gcgcaagacc accaacgatc tgtcggacgc
     1501 gctgcacggc gatcgccacg gccaggagac cgaggccgag aacctgttcc aggactgagc
     1561 gcgcggcgtg cgagcgctac gcggcgcgct ggaacaggaa gtagtcgcac ggcacctcga
     1621 tgacctcgcc gccgggcagc gcctcggtgg tgaccaccgt cgtggttccc tgggcgcgca
     1681 gcgcacagcc tgcgtgatgg aacagcgccg ggacctctcc tgcctgcagc gacgccggcg
     1741 cgatgccgtg tgacagcgcg tactgcagct tgccgggtgg gccgccgaac cgctgcgcca
     1801 gcgcggcgaa cagctgaccc gccgccgggg acagctcgac caggaatgcc agacctcgct
     1861 cgccgagcag cgccgccagg tggtccacgg ccggcgtccg atccgcggcg cgcatctggt
     1921 gcagcacgcc gcgcaggtag aggttggcgt cgcccagctc ggcgtgcagc gcgcggatgg
     1981 ccgccccgtc gagcagatcg agcgcgcggt actcgaggtt ggccgccccg tggtactggc
     2041 gcgcgccgcg cagcgcctcc tcggagacat cgacgccgat cacccgagaa aacgcatccg
     2101 ccaggcagcg cgtctgcgtt ccgttgccac agccgacatc gatgaccggc agtgcccggt
     2161 cgaacgcgct cgcgaacacc ggcagctcgc gctgcacggc ccggtccggc gtcgagtccc
     2221 agaacgcgac acccggccgc ggcgtcaccg cgcgccagta gccatcccag gactcctggt
     2281 agctgcgcgt catgatcggc ggctcctgat gatcggcatc gcgcctcgtg ctcccgtccg
     2341 gtgctcccca ccgtgataca ccatcccggc gtgcgcagga cgatcagact caccgtcggg
     2401 ctcgcgctcg ggctcgcgct cgccgcgtgc ggccatgcgg cttcgtcgcc gagctcgccg
     2461 ggtgcgcccg gaccacggat cgtcgcgatg atctcggcca atgccgagtg gaagatcgtg
     2521 cgcgcccagc tgcccgaggc gccgcaccac gacacgccgt tcggcgagtg gctggtccac
     2581 cgggtcggcg ggcaggacgt cgtgttcttc cacggcggct acggcaaggt cgccgcggcg
     2641 ggctcgaccc agtacgcgat cgaccgctgg caccctgtcc tgctggtcaa cctcggcacc
     2701 tgcggcgggt tcggcgacgg gcgcaaggtc ggcgacatcg tgctcgcgag cgagaccacg
     2761 atctacgaca tcgtcgagct gatgggcgat cccgacgagg cgatcgcgga cttccgcacc
     2821 cggctcgaca cctcgacgtg gcccgcccgg ctcgccggcc gcgtggtcat cggcccgatc
     2881 gtctcggcgg atcgcgacct cgatcccgcc gcggtcgccg gcctcgcggc gaagtaccac
     2941 accggcgtcg gcgactggga gtccggggcg atcgcctggg tcgcgtcgca caaccacacg
     3001 ccggtgctga tcctgcgcgg cgtcaccgat ctcgtcgatg cgcccggtgg cgatccgacg
     3061 tacaacgccg tggatgtctg gcagcgcgcc agcatcgcgg tcatggcctc gctggtcgcg
     3121 ctgctcggcg acgcgctgcc cgacgtgctg ccctcgctcc ggtgaccacg ccggggccgc
     3181 gcgtcagcgg aaggtcgagc gcctgcagaa gaagccgagt gacccgttgc agccttcgtc
     3241 gtcccacgtt ccgccgggcc ggatcgtgcc gcactgctct gcgccgtcct cgttgccatc
     3301 ggcgtcgtcc ggcttgttgg tcccccagtt gatgtaccgt gtggagttga acagcttggt
     3361 agcgtccccg ttccaggtcc acttgtcgcc cggcgtcatc gccccggcgc cctggatcgc
     3421 tccgaaccag aacgtggcgg tgctgatctt catggtaacg cagccgttct cggtcgcgtc
     3481 atcgatctcg cccagcgcgc cggtccagcc ggtgcaccgg gtctcggccg tcgcgcgcga
     3541 caccaggtcg gtgcacttca cccagcactg ggtgccgcac aggaacacgg tgggggtccc
     3601 accgcaggtc agcccgtcgg catcgcacgg cacaggcatc gcatcgacca tcgcatcgat
     3661 catctgcgca tcgggcggcg gagtcgggtg cgcatcgacc ggcggcggcg cgctccgcga
     3721 gcagctaccg agcacgcact gctgcggttc ggggcactgc tccgagcgct gacacggcgc
     3781 cccctccggc agcgccgatg ggttgtagca gccggcgaat ccgaccagcc acatcgccga
     3841 ggcgaggacc cggatcggcg ataccgcgaa gccgg
//
LOCUS       DASVOR010000444         2052 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_559586,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000444 DASVOR010000000
VERSION     DASVOR010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559586"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VF516_08855"
     CDS             <1..1783
                     /locus_tag="VF516_08855"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8107828.1"
                     /translation="GGDILDYRRRITELGPRQFFDTVSTIRGVVGVTGSVPASLGMFE
                     NWNYEVSFNYGETHSLVGTDGQLLLPRLVDALGPSMRDAGTPICVRVPGDPSTKITYN
                     VPLASGTTLHAACVPLNMLAPAGAIPRDQLKNVTFDDVGTGTDTVRTYLATASGRVAE
                     LPDHGEISLSIGGDARYELGEHEPPEVASAGYTTDNEAHDTIGRFHLFEGFGELAIVP
                     ITDGDLAQRVEIDLGARALHHNRYGSSLTYKAGALVRTAGGIAVRGTYATAFRSPSVF
                     DLFGGRTERMPTVEDPCDARPPSAGDGTRTLDPRVQAQCTAQGIPVGTRFNTGQQPAV
                     IGGNPDLQAETAATATVGVVIEPPQLPGLALTADYWHIAIEDAIETLGVATIFASCYD
                     RGIQAFCDQIHRDFNTHRILHVDQFLQNVSRTVTSGVDVALWYDARLAQLGRIHTGLE
                     AQYLLRYDLETGNQVIHGVGFYDLGVYPRYKANLSSRWTHPGGASGGFTLRYVGSYQE
                     CAGDDCNTPHNLAVASRAVDRYVKLDLFGGYDLPSWLGKTTLQAGINNVFDATPPVVY
                     NAPAANSDATTYDFLGRMVYLRMSQLF"
BASE COUNT          310 a          825 c          636 g          281 t
ORIGIN      
        1 cggcggcgac atcctcgact accggcgccg gatcaccgag ctcggcccgc ggcagttctt
       61 cgacaccgtc agcacgatcc gcggcgtcgt gggcgtcacc ggcagcgtcc ccgcgtcgct
      121 ggggatgttc gagaactgga actacgaggt gtcgttcaac tacggcgaga cccattcgct
      181 cgtcgggacc gacggccagc tgctcctgcc ccggctcgtc gacgcgctcg ggccgagcat
      241 gcgcgacgcc ggcaccccga tctgcgtccg tgtgccgggc gacccgtcga ccaagatcac
      301 ctacaacgtg ccgctcgcca gcggcaccac cctccacgcg gcgtgcgtcc cgctcaacat
      361 gctcgcgccc gccggcgcga tcccgcgcga ccagctcaag aacgtgacgt tcgacgacgt
      421 gggcaccggg acggacaccg tgcgcaccta cctcgccacc gcgagcggcc gcgtcgccga
      481 gctgcccgac cacggcgaga tctcgctgtc gatcggcggc gatgcgcgct acgagctcgg
      541 cgagcacgag cccccggagg tcgcgagcgc cggctacacc accgacaacg aggcccacga
      601 cacgatcggc cggttccatc tgttcgaggg cttcggcgag ctggcgatcg tcccgatcac
      661 cgacggcgac ctcgcccagc gggtcgagat cgacctcggc gcccgcgcgc tccaccacaa
      721 ccgctacggc tcgagcctga cctacaaggc cggcgccctg gtccgcaccg ccggcggcat
      781 cgccgtgcgc ggcacctacg ccaccgcgtt ccgctccccg tcggtgttcg acctgttcgg
      841 cggccggacc gagcgcatgc ccaccgtcga ggacccgtgc gacgcccggc cgcccagcgc
      901 cggcgacggc acccggaccc tcgatccgag ggtccaggcc cagtgcaccg cgcagggcat
      961 cccggtcggc acccggttca acaccggcca gcagccggcc gtgatcggtg gcaaccccga
     1021 cctccaggcg gagaccgccg ccaccgccac ggtcggcgtg gtgatcgagc cgccccagct
     1081 cccgggcctc gcgctcaccg ccgactactg gcacatcgcc atcgaggacg cgatcgagac
     1141 cctcggcgtc gcgacgatct tcgccagctg ctacgaccgc ggcatccagg cgttctgcga
     1201 ccagatccac cgcgacttca acacccaccg gatcctccac gtcgaccagt tcctgcagaa
     1261 cgtctcccgc accgtcacgt ccggcgtcga cgtcgcgctg tggtacgacg cccggctcgc
     1321 ccagctcggc cggatccaca ccgggctcga ggcccagtac ctgttgcgct acgacctcga
     1381 gaccgggaac caggtgatcc acggcgtcgg gttctacgat ctcggcgtct acccccgcta
     1441 caaggccaac ctgtcgagcc gctggaccca ccccggcggc gcgtccggcg gcttcaccct
     1501 gcgctacgtc ggctcgtacc aggagtgcgc cggcgacgac tgcaacaccc cccacaacct
     1561 cgccgtggcg tcgcgcgccg tcgaccgcta cgtcaagctc gacctgttcg gcggctatga
     1621 cctgccgtcg tggctgggca agaccaccct gcaggccggc atcaacaacg tgttcgatgc
     1681 caccccgccg gtggtctaca acgcccccgc cgccaactcc gacgccacga cctacgactt
     1741 cctcggccgc atggtctacc tccggatgtc ccagctgttc tgaccggcag gctccgtgcc
     1801 gtgatgcacc ccggccgcac gcgctgtcgc gggacatccg ctgcggcgcg cgggcggcgc
     1861 gacctataca atgttggccg tttgccaggc gtttctgctc acctcgggca gcaaccgacg
     1921 catgatggca ctgcgcggag catggggatg acgaagcacg ctgcgatcct gtgcctggtg
     1981 gcggtcgccg caccggccgc tgccgacgac cgcgcgccca gctcgctgcg catgcgcgac
     2041 acgccgcccg at
//
LOCUS       DASVOR010000445         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412672,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000445 DASVOR010000000
VERSION     DASVOR010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412672"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VF516_08860"
     CDS             complement(<1..987)
                     /locus_tag="VF516_08860"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8107829.1"
                     /translation="MEHLDDNAASEFVSGTLSPSALSKVEGHLASCRECRALVAALAA
                     DPATDSAAVTFQHLTSVSSVSELPRRPPSSGDRVGRYLVLSALGAGGMGVVFAAWDPQ
                     LGRKIALKLLRSSVASSSKDARTRLRREAQAIAQLSHPNVVSIYDVGTTDDGALYIAM
                     EFVEGDTLTSWLKSYPRTWRETVDVFRQAARGLLAAHSVGLLHRDFKPDNVLVGGDGR
                     VRVTDFGLARSVLVPDEAAQAKPVVSALDVALTATGTVLGTPRYMPPEQLTGPDLDAR
                     SDQFSFCIALYEALYGRHPLPGGTSVAMLDHGERAAAPPAGSKVPPALARAVL"
     gene            1149..>1682
                     /locus_tag="VF516_08865"
     CDS             1149..>1682
                     /locus_tag="VF516_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8107830.1"
                     /translation="MLTDLSKVRNIGISAHIDSGKTTLTERILYYTARIHAIHEVKGK
                     DGVGAKMDSMELERERGITIQSAATFCQWPPGEKSKGVMAVPGTPIKAQHHINIIDTP
                     GHVDFTIEVERSLRVLDGAIMVLCGVAGVQSQSYTVDRQMRRYKVPRIAFVNKLDRTG
                     ANPYRVRDQLRDKLKLHP"
BASE COUNT          273 a          591 c          584 g          234 t
ORIGIN      
        1 cagcaccgcg cgggccagcg ccggggggac cttgctgccg gcgggcggcg ccgccgcgcg
       61 ctcgccgtgg tcgagcatcg ccaccgaggt gccgccgggc agcgggtggc ggccgtacag
      121 cgcctcgtag agcgcgatgc agaagctgaa ctggtcgctg cgcgcgtcga gatcggggcc
      181 ggtcagctgc tccggcggca tgtagcgcgg cgtgccgagc accgtgccgg tcgcggtcag
      241 cgcgacgtcg agcgccgaca ccaccggctt ggcctgcgcg gcctcgtcgg gcaccagcac
      301 cgagcgcgcg agcccgaagt cggtgacccg gacccggccg tcgccgccga ccagcacgtt
      361 gtcgggcttg aagtcgcggt gcaagaggcc gacgctgtgc gcggcgagca ggccgcgcgc
      421 cgcctggcgg aacacgtcga cggtctcgcg ccaggtccgc gggtagctct tgagccagct
      481 ggtcagggtg tcgccctcga cgaactccat cgcgatgtac agcgcgccgt cgtcggtggt
      541 gccgacgtcg tagatcgaca ccacgttggg gtgggacagc tgggcgatcg cctgggcctc
      601 gcggcgcagc cgggtccgcg cgtccttgct cgagctcgcg acgctcgacc gcaggagctt
      661 gagcgcgatc ttgcggccga gctgggggtc ccaggcggcg aacaccacgc ccatgccgcc
      721 ggcgccgagc gccgacagca cgaggtagcg gccgacccgg tcaccgctcg acggcggccg
      781 cctcgggagc tcgctgaccg acgacaccga cgtcaggtgc tggaacgtca cggcggccga
      841 gtcggtcgcc ggatcggcgg cgagcgcggc gaccagcgcg cggcactcgc ggcagctcgc
      901 gaggtggccc tcgaccttgc tcagtgccga cggagacagc gtgccggaca cgaattcgct
      961 cgcggcgttg tcgtcgaggt gctccaccga tccgaaaagg tagcaaacga ccccgatcaa
     1021 ctctttgaaa accaatcaca tccgcgtggg gtcggttgtg accactgcgc ccttgcgtgt
     1081 gagccggatc attggtagca atcccgcgcc tttatcgggg ctcttcggag cttccaggag
     1141 acactttcgt gctcaccgac ctgtccaaag tccgcaacat cggcatctcg gctcacatcg
     1201 acagtggcaa gacgacactg accgaacgga tcctgtacta caccgcccgg atccacgcga
     1261 tccacgaggt caagggcaag gatggcgtcg gcgccaagat ggactcgatg gagctggagc
     1321 gcgagcgcgg catcacgatc cagtcggctg ccacgttttg ccagtggccg ccgggcgaga
     1381 agtccaaggg cgtgatggcc gtgccgggca ccccgatcaa ggcccagcac cacatcaaca
     1441 tcatcgacac cccgggccac gtcgacttca cgatcgaggt cgagcgctcg ctgcgcgtgc
     1501 tcgacggcgc gatcatggtg ctgtgcggcg tcgccggcgt ccagtcgcag agctacaccg
     1561 tcgaccgcca gatgcggcgc tacaaggtgc cgcgcatcgc gttcgtcaac aagctcgacc
     1621 gcaccggcgc caacccgtac cgcgtgcgcg accagctgcg cgacaagctc aagctccacc
     1681 cg
//
LOCUS       DASVOR010000446         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501044,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000446 DASVOR010000000
VERSION     DASVOR010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501044"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..699)
                     /locus_tag="VF516_08870"
     CDS             complement(<1..699)
                     /locus_tag="VF516_08870"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8107831.1"
                     /translation="MTDLILHHFDQSPFSEKVRLVLGLKQLSWRSVKVPVRLPKPDVV
                     ALTGGYRRTPFLQIGADLYCDTALMCRVIDRLAPEPPLYPAAATGLQHVLAQWADTAL
                     FWAAVPYTLQPAGFAHAFGQVSPEEIQALAADRAAMTAGMRRATLIDATAQLTSYLGW
                     LESMLSGGQAFLLGDRPCIADVAVVHSLWFIRLAPPVAGVLAPFGKLAAWYDRVIAFG
                     HGRDEPLGSGDAIAI"
     gene            complement(1078..>2018)
                     /locus_tag="VF516_08875"
     CDS             complement(1078..>2018)
                     /locus_tag="VF516_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107832.1"
                     /translation="EILPAAPFVDGIAVRDSGAARVKIRTLSHLIWALGLAAFAGVLA
                     EVALRWFAPTWSVSYRLMRLEGVEPALAAMNVSYLAGTQLALTCGYLGTALMALSMAY
                     LLQRRFGWFHRTATNQFWLDVHLMTGTVGPLFIVLHSALRLTTWVSIPFWSMVAVVIS
                     GVLGRYLYTLVPSLTSKHDLVILAHRRAITELAADHPAAGDHAYQVMDREARRSEAAW
                     QIGLVPLLGWLLADDLRRRWTRRRDHRALARLAPRKIARRIARRIDQVVVLERRKELA
                     PRGKALLRSWKRVHVPFSIVLLVTMLVHIALALHLG"
BASE COUNT          313 a          671 c          763 g          271 t
ORIGIN      
        1 gatcgcgatc gcgtcgcccg accccagcgg ctcgtcgcgg ccgtggccga acgcgatgac
       61 ccggtcgtac cacgccgcga gcttgccgaa cggcgcaagc accccggcga ccggcggcgc
      121 gagccggatg aaccacagcg agtggacgac cgcgacgtcg gcgatgcacg gccggtcgcc
      181 gagcaggaac gcctggccgc cgctcagcat cgactccagc cagcccaggt agctggtgag
      241 ctgcgcggtc gcatcgatca gcgtcgcccg gcgcatcccg gcggtcatcg cggcgcgatc
      301 cgcggccagc gcctggatct cctcgggcga gacctggccg aacgcgtgcg cgaacccggc
      361 cggctgcagc gtgtacggga ccgcggccca gaacagcgcg gtgtcggccc actgcgccag
      421 cacgtgctgc aggccggtgg ccgccgccgg atacagcggc ggctcgggcg cgagccggtc
      481 gatcacgcgg cacatcagcg ccgtgtcgca gtagaggtcg gcgccgatct gcaggaacgg
      541 cgtgcggcgg tagccgccgg tcagcgccac cacgtccggc ttgggcagcc ggaccggcac
      601 cttgaccgac ctccagctca gctgcttgag gccgagcacc aggcggactt tttccgagaa
      661 cggcgattgg tcgaagtggt gcaggatcag gtcggtcatg gttccccgcg cggtgtggat
      721 cgcgcggcgg attgtcgctc atcccggtga tggctgggcg cccggcgtat gagatctttg
      781 tcggggtgct atcgtcggcg cctcgggacc ctgaaccgat gcgggcaggt caggagtgcg
      841 tcgatcagcc cttcgttcgg ccgccgcgcg agctttcgca tcacgatcac gttcggatca
      901 tcgctgtcca ggaactcgag ggtgtccccc ggccggacac gagctcgttc gcgcagcttc
      961 tgaggcagcg cgatctgccc agtaggggag accttggcgg ttcgcatctc ggcaagttga
     1021 acttacccga gatctgcgac tgggaagctg ctcggcgggc gcgccgcgac tcgcggatca
     1081 tccgaggtgg agcgcgagcg cgatgtggac gagcatcgtc accagcagga cgatcgagaa
     1141 tggcacgtgg acgcgcttcc acgaccgcag cagcgccttg ccgcgcggtg cgagctcctt
     1201 gcggcgctcg agcacgacga cctgatcgat ccggcgcgcg atccggcggg cgatcttgcg
     1261 cggcgcgagg cgggcgaggg cgcggtggtc gcggcgacgg gtccaccgcc ggcgcagatc
     1321 gtcggcgagc agccagccga gcagtgggac gaggccgatc tgccaggcgg cctcggagcg
     1381 gcgggcctcg cgatccatca cctggtaggc gtggtcgccg gcggcggggt ggtcggccgc
     1441 gagctcggtg atcgcgcggc gatgggcgag gatcacgagg tcgtgcttgc tggtcagcga
     1501 cgggacgagg gtgtagaggt agcggccgag cacgcccgag atcacgacgg cgaccatgct
     1561 ccagaacggg atcgacaccc aggtggtcag gcgcagcgcc gagtgcagca cgatgaacag
     1621 cgggccgacg gtgccggtca tcaggtggac gtcgagccag aactggttgg tcgcggtccg
     1681 gtggaaccag ccgaaccggc gctgcaggag gtacgccatc gacagcgcca tcagcgcggt
     1741 gccgaggtag ccgcaggtca gcgcgagctg ggtgccggcg aggtagctga cgttcatcgc
     1801 ggcgagcgcg ggctcgacgc cttccaggcg catcagccgg tagctgaccg accaggtcgg
     1861 cgcgaaccag cgcagcgcga cctcggcgag cacgccggcg aacgccgcga ggcccagggc
     1921 ccagatcagg tggctgagcg tgcggatctt gacccgcgcg gcgcccgagt cgcggaccgc
     1981 gatgccgtcg acgaacggcg cggcgggcag gatctctc
//
LOCUS       DASVOR010000447         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412676,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000447 DASVOR010000000
VERSION     DASVOR010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412676"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VF516_08880"
     CDS             complement(<1..534)
                     /locus_tag="VF516_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8107833.1"
                     /translation="MLTDLSKVRNIGISAHIDSGKTTLTERILYYTARIHAIHEVKGK
                     DGVGAKMDSMELERERGITIQSAATFCQWPPGEKSKGVMPVPGTPIKAQHHINIIDTP
                     GHVDFTIEVERSLRVLDGAIMVLCGVAGVQSQSYTVDRQMRRYKVPRIAFVNKLDRTG
                     ANPYRVRDQLRDKLKLHP"
     gene            696..>1548
                     /locus_tag="VF516_08885"
     CDS             696..>1548
                     /locus_tag="VF516_08885"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8107834.1"
                     /translation="MEHLDDNAASEFVSGTLSPSALSKIEGHLASCRECRALVAALAA
                     DPVADSAAVTFQRLMSLSSVSSVGELPRRPPSSGDRVGRYLVLSALGAGGMGVVFAAW
                     DPQLGRKIALKLLRSSVASSSKDARTRLRREAQAIAQLSHPNVVSIYDVGTTDDGALY
                     IAMEFVEGDTLTSWLKSYPRTWREIVDVFRQAARGLLAAHSVGLLHRDFKPDNVLVGG
                     DGRVRVTDFGLARSVLVPDEAAQAKPVMSALDVALTATGTVLGTPRYMPPEQLTGPDL
                     DARSDQFS"
BASE COUNT          217 a          516 c          551 g          264 t
ORIGIN      
        1 cgggtggagc ttgagcttgt cgcgcagctg gtcgcgcacg cggtacgggt tggcgccggt
       61 gcggtcgagc ttgttgacga acgcgatgcg cggcaccttg tagcgccgca tctggcggtc
      121 gacggtgtag ctctgcgact gcacgccggc gacgccgcac agcaccatga tcgcgccgtc
      181 gaggacgcgc agcgagcgct cgacctcgat cgtgaagtcg acgtggcccg gggtgtcgat
      241 gatgttgatg tggtgctggg ccttgattgg cgtgcctggc acgggcatca cgcccttgga
      301 cttctcgccc ggcggccact ggcagaacgt ggcagccgac tggatcgtga tgccgcgctc
      361 gcgctcgagc tccatcgagt ccatcttggc gccgacgccg tccttgccct tgacctcgtg
      421 gatcgcgtgg atccgggccg tgtagtacaa gatccgttcc gtcagtgtgg tcttgccact
      481 gtcgatgtga gccgagatgc cgatgttgcg gactttggac aggtcggtga gcacgaaagt
      541 gtctcctgga agccccgaag agccccgata aaggcgcggg attgctacca atgatccggc
      601 ttcgacgcaa gggcgcagtg gttacaaccg accccacgcg gatgtgattg gttttcaaga
      661 agttggtggg gggcgcctgc taccctttcg gacgggtgga gcacctcgac gacaacgcgg
      721 cgagcgaatt cgtctccggc acgctgtctc cgtcggcgct gagcaagatc gagggccacc
      781 tcgcgagctg ccgcgagtgc cgcgcgctgg tcgcggcgct cgccgccgat ccggtggccg
      841 attcggccgc ggtgacgttc cagcgcctga tgtcgctgtc ctcggtgtcg tcggtcggtg
      901 aactcccgag gcgcccgccg tcgagcggcg accgggtcgg ccgctacctc gtgctgtcgg
      961 cgctcggcgc gggcggcatg ggcgtggtgt tcgccgcgtg ggacccgcag ctcggccgca
     1021 agatcgcgct caagctcctg cgctccagcg tcgcgagctc gagcaaggac gcgcggaccc
     1081 ggctgcgccg cgaggcccag gcgatcgccc agctgtccca ccccaacgtc gtgtcgatct
     1141 acgacgtcgg tacgaccgac gacggcgcgc tgtacatcgc gatggagttc gtcgagggcg
     1201 ataccctgac cagctggctc aagagctacc cgcggacctg gcgcgagatc gtcgacgtgt
     1261 tccgccaggc ggcgcgcggg ctgctcgccg cgcacagcgt gggcctgttg caccgcgact
     1321 tcaagccgga caacgtgctg gtcggcggcg acggccgggt ccgggtcacc gacttcggcc
     1381 tcgcgcgctc ggtgctggtg cccgacgagg ccgcgcaggc caagccggtg atgtccgcgc
     1441 tcgacgtcgc gctgaccgcg accggcacgg tgctcggcac gccgcgctac atgccgccgg
     1501 agcagctgac cggccccgat ctcgacgcgc gcagcgacca gttcagct
//
LOCUS       DASVOR010000448         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501057,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000448 DASVOR010000000
VERSION     DASVOR010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501057"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            556..1956
                     /locus_tag="VF516_08890"
     CDS             556..1956
                     /locus_tag="VF516_08890"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF005450.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8107835.1"
                     /translation="MAAIHELTAHELVAAFTQRTLSPLDVTRAALARIDAWEPRIRAM
                     YRIARDAALDQARAAEARWRDGAPRSALDGVPITIKENIYTLGDPAPIGTRANEHAPP
                     QPTDAPAAARVREAGLVLLGKTTMPDYGMLSSGLSSLHGVTRNPWRLDRNPSGSSSGA
                     AAAAVAGYAPLHIGTDIGGSVRLPATHCGIFALKPSLGRIPVYPPYLGRVAGPMTRTV
                     ADAALLLNVLSRPDPRDFMSLPYAPHDYLQGLDQLDVRGLKIGLLPDLHVGLPVDPEV
                     RAAAQAAAKALAGAGAVVDEMPSFLTADMLDGMCRFFEARLYNDFAALPAERQRAVLP
                     FVAEWCTWRAARFTGRDVTQAFGHVMAMREAAVFACQPFDYVISPVSPILPYDAELPA
                     PNNDPHDALAHIAFTVPFNMSEQPAASINWTASQGGLPIGVQIIGRRFDDAGVLRVAR
                     LIERLRPAQRAWPEPA"
     gene            2891..>3142
                     /locus_tag="VF516_08895"
     CDS             2891..>3142
                     /locus_tag="VF516_08895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107836.1"
                     /translation="MLLHSESPQEREGQTADPWRSEWSEVHRRLRDYARHRASLDAAE
                     AFDLVRAEQLKLHFLCGFATMYEYMERVLGYGPHAARERL"
BASE COUNT          475 a         1101 c         1052 g          514 t
ORIGIN      
        1 tccgctggct tgcaccgaga atcctgacta ttgactcacc tttggcacgg ggtgtgcttc
       61 caagacatag cttcgaagga gtccctttcc gcttacactg gattatttgc tcacagtaat
      121 tttccgatgg gattgtttgt acgccagcct ttgggtatct cgcaatacga aagtgacttt
      181 cgttttttcg agatcggcta acgagccctg tcacgacgcg gagttcgctc gggttggcaa
      241 acctcatcgg tgtgagctct gccacaaatc gttcgtgagc cggagataac ggtggaagac
      301 ttttccatcg gatctgctgc atggcgtggc atgcctgaaa acgctgcatg ctacgcaaat
      361 ctgatgctga gtcagagtcg cgctccattg tgagatcgcc tgactctctt ccagacatgc
      421 tccgcctgtt tcgcacgtgc ggagagcctc acgcaacaac tgccccaggg ttctggacag
      481 gaaggattgg acggaccacc ggccactgga aggcgttccc tgtaggccct cgcgcggcga
      541 tatggtcccc gcacgatggc cgccattcac gagctcaccg cgcacgaact cgtcgccgca
      601 ttcacccagc gaacgctgtc gccgctcgac gtcacgcgcg ccgcgctcgc gcggatcgac
      661 gcctgggagc ccaggatccg cgcgatgtac cgcatcgcgc gcgacgccgc gctcgatcag
      721 gcgcgcgcgg ccgaggcgcg ctggcgcgat ggcgcgcccc ggtccgcgct cgacggcgtc
      781 ccgatcacca tcaaggagaa catctacacg ctcggcgatc cggccccgat cggcacgcgc
      841 gccaacgagc acgctccccc gcaacccacc gacgcgccgg ccgcggcccg ggtccgcgag
      901 gcggggctgg tgctgctcgg caagaccacg atgcccgact acgggatgct gtcgtcgggg
      961 ctgtccagcc tgcacggcgt gacccgcaac ccgtggcgcc tggaccgcaa cccgtcgggc
     1021 tcgagctcgg gcgcggcggc ggcggccgtc gcgggctatg cgccgctcca catcgggacc
     1081 gacatcggcg gctcggtccg cctgcccgcc acgcactgcg gcatcttcgc cctcaagccg
     1141 tcgctcggcc ggatcccggt ctatccgccc tatctcgggc gtgtcgccgg gccgatgacc
     1201 cgcaccgtgg ccgacgccgc gctgctcctg aacgtgctgt cgcggcccga tccgcgggac
     1261 ttcatgagcc tgccgtacgc gccccacgac tacctccagg gcctcgacca gctcgacgtc
     1321 cgcggcctga agatcggcct cttgcccgac ctgcacgtcg gcctgccggt cgaccccgag
     1381 gtccgcgccg ccgcccaggc cgccgcgaag gcgctcgccg gcgccggcgc ggtggtcgac
     1441 gagatgccgt cgttcctgac cgccgacatg ctcgacggca tgtgccggtt cttcgaggcc
     1501 cggctgtaca acgacttcgc ggcgctgccc gcggagcgcc agcgggccgt gctgccgttc
     1561 gtcgccgagt ggtgcacctg gcgcgccgcg cggttcaccg ggcgcgacgt cacgcaggcg
     1621 ttcggccacg tgatggcgat gcgcgaggcc gccgtgttcg cctgccagcc gttcgactac
     1681 gtcatctccc cggtgtcgcc gatcttgccc tacgacgccg agctccccgc gcccaacaac
     1741 gatccgcacg acgcgctggc ccacatcgcg ttcaccgtgc cgttcaacat gtccgagcag
     1801 cccgcggcat cgatcaactg gaccgcgagc cagggcggcc tgccgatcgg cgtgcagatc
     1861 atcggccggc ggttcgacga cgccggcgtg ctccgcgtcg cgcgcctgat cgagcggctg
     1921 cggccggccc agcgcgcctg gcccgagccg gcatgaccgg cggccgatgg ccatggtctc
     1981 agcggcggtc cctggatgca gggcgtgtac ccgcaggtgc atccggtgca ccaggtgcac
     2041 cgggacgacg ccccacctgc ggcggctgcg agctcctgct cgtcgaggct cctgaggttt
     2101 gccaccgccg gctttgcgag cacgagtcgc cgcttgtgca tctcgcttct tcttcatgtc
     2161 gacccctcca tcccctcgtt ggacacgtac gcggcggatc caatccaagc gcgatgccat
     2221 cgtgcgggcg gcgcgatgcc gtggcgctgc cggcgtgtgg ttggtacggc caggcacctg
     2281 tgccggcggc cggccagcgg ccagccgcgt gccggcggcc ccggggagct cacccgagga
     2341 cggagcgcga aggattcgcg atcgctctca gtcgcttgcg tcgtcgcggc gggcggcggc
     2401 tgcgggagcc gtgaggatgc cgcgcgccga gcatggtccc tgcggtcgcc gcgaggtgtc
     2461 gcgcggccac ggtcgcagca ggcattgctc gatgaacgac gcgtgcacgg tcgccggcgg
     2521 cgacagcccg gcgagcgcgg tcgcgatcaa gcctcggtcg cggcctgggc gagcgcgtcc
     2581 tgcatcggcg gagtcttcgg ctcgatcacc gagcggcgat ggagcgagca ggcaaagcat
     2641 agaaattcgt agtcccatga cgattccgat tgacagcgcc gggatggtcc gcatggagcg
     2701 aatcgagaac cttcatttga tccagcgcag tgagagtcag atagcgacag gcgcgcatga
     2761 ttcgagtggg gttgctcgaa atcgccggca gggtcatttc cgtgcgggag atcgcagcag
     2821 gatgcgtcga aaaacaccgt gctacagagg tcacacacgc gagccagggt gtctgcgaaa
     2881 gggaacgtcg atgttgctgc acagcgagag cccccaggag cgagagggac agaccgccga
     2941 tccgtggcgg tcggagtgga gcgaggtgca tcgccggctg cgcgactacg cgcgccaccg
     3001 cgcctcgctc gatgccgccg aggcgttcga cctggtgcgc gccgagcagc tgaaactcca
     3061 tttcctgtgc ggcttcgcca ccatgtacga gtacatggag cgcgtgctgg gctacggccc
     3121 gcatgcggcg cgcgagcggc tg
//
LOCUS       DASVOR010000449         5965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_237767,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000449 DASVOR010000000
VERSION     DASVOR010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237767"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..280
                     /locus_tag="VF516_08900"
     CDS             47..280
                     /locus_tag="VF516_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX8107837.1"
                     /translation="MDKTRRDAARLTEVLEGAGIPYKVMNIQDDPAAQSAVRRDSKGF
                     RLPVVFIAGEVIGGRAELTNLAASGELKKRVFG"
     gene            338..724
                     /locus_tag="VF516_08905"
     CDS             338..724
                     /locus_tag="VF516_08905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107838.1"
                     /translation="MMAAQHKVLLIDDSEISLHFVAGVLLRAGYDVRTADDVDKLEGV
                     LGDWRPDVILTDVHMPGISGIELCRMLKSNYETAHVPVVLFSAVPQHELEGMARDCEA
                     DGCLSKSNGLERLPKELQLLIETALF"
     gene            complement(1028..3790)
                     /locus_tag="VF516_08910"
     CDS             complement(1028..3790)
                     /locus_tag="VF516_08910"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8107839.1"
                     /translation="MRGEVVNAGATAGQLYLVARDADELVATIAASHPRAVIIDREYG
                     EADDVLRRLTDLVEVPELRDRERTTLFQVIRDTSQEPAPGADVIVVEPALRHALALTD
                     HALAERRPLALDRLLTISVLSGPLDDALEAAAAQLAVGFGVDRCVISVRGDSTGAAVG
                     SHTWDSLTWNQTADYCRIATATGASLIAPSPVKGGACESYLAVPLDTPLGSHGFVGLI
                     ITRPRIFSRDHRTVLQSIASRLGAELGWRAVHQRTTDELDRVVNGPGLDQLLGIWNRT
                     ATAQLATMHVSASHRSGLPLTAVLVDILDLREINTRHGLAVGDRVLCRVADAIRANVR
                     TEDVVGRWSGDKIAVLLPGTPIDGSQRVAERFRAAIADRPLELPTGGTLQIPATVGLA
                     ALQKNEEAAMLVTRAAHAAKKAQGSDRPIARASTGPAPRLSTQQIDLGEDMRATLGGT
                     YRLLHEISRGGMGVVYRAEDLALERPVAIKMLRPDLAEDKAFVEHLRGEAAMLARLEH
                     PNLVQIYNFGQSGGDSYFVMELLEGEGLQQAVERHRIEGTLMPIGELVTVVDQVASAL
                     DALHERGIIHRDVKPANVIRDPFRSRSVLLDVGIARRYGQFAESAGTPGYVAPEVIEG
                     FEATPRSDVYGLAATAYTLLTLTPPWGEGEGVLQRQCDGEDLVPPSVIRAELEPADEV
                     LTRGMSRDPGERPISAGAFARALRAALIGVDPLPKPDGGRWVANTVLPRSGSPVPKTR
                     GVVFRSVARALGVREAERLRDAIGGEHTELARALTDTAPLAWLPTELFIRLLTIAPEH
                     VQRDSARLAHDIARATVRASFRRFFPASAATLVPERTLSAIRNVWSRYQSWGTVSSMP
                     IHPNETVVRITGTPRNPELCMWTTGMLEQLVVLSGGRGVAVDHEACEARDDDACLFRV
                     MWGRPE"
     gene            4283..4780
                     /locus_tag="VF516_08915"
     CDS             4283..4780
                     /locus_tag="VF516_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107840.1"
                     /translation="MVDDDERILGSWKRAARERNVFTAIDAATARQLASTEKPDLAIV
                     DLRLGNTSGIDLIRDLKRDLPDLLVVLCSGYLSVAAAVAAVRAGADIVVFKPITFREI
                     LQRIEQNSEEPDLEDTPTLARAEWEHIMRVLADCNGNVSMAARRLGIYRSSLQRRLRK
                     YAPRS"
     gene            5093..>5965
                     /locus_tag="VF516_08920"
     CDS             5093..>5965
                     /locus_tag="VF516_08920"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107841.1"
                     /translation="MAAANSERFIVRAEVRLGDDTVISYGFDLTSSSVFVVTEWHAPI
                     GTPVALRLSFPRILEPIDVVARVADIRVAGEPGEPGGVRLAFDEGEVGARLMSQLEHT
                     GGADPAADRPYRVLLVDDSWLIRDMFAHGTAKFFDRPGTLAIDGAENAEEAWSKLAKS
                     TYDLLIVDYFLPAEDGASLIARLRADARLARTPIVAISVGGRDAREATISAGADVFVD
                     KPLVFRDLFNTLRVLAEHRERSRPPAERKAILVFDDSPFALALTRAALEAAGFTVAIA
                     ADLSSFERQRVAFDP"
BASE COUNT          852 a         2052 c         2175 g          886 t
ORIGIN      
        1 gtcttcaagc gagccgcgtt aggccgcgcc ggtcgtcgtt taccacatgg acaagacgcg
       61 ccgcgacgcg gcccggctga ccgaggtcct cgagggtgcg ggcatcccct acaaggtaat
      121 gaacatccag gatgacccgg cggcgcagtc cgcggtccgg cgcgatagca aggggttccg
      181 gttgcccgtg gtgttcatcg cgggggaggt catcggcggg cgcgccgagc tgaccaacct
      241 ggccgcgtcc ggtgagctga agaagagggt attcggctag gacggcgatc gagcaccgcc
      301 gcgtggtcgt gggtccgtgc taaggtctcc gcctcggatg atggcagccc agcacaaggt
      361 cctgctcatc gacgacagcg agatcagcct gcacttcgtg gccggcgtgc tcctgcgggc
      421 gggctacgac gtccgcaccg cggacgacgt cgacaagctc gagggcgttc tcggcgactg
      481 gcggccggat gtgatcctga ccgacgtcca catgcccggc atctccggca tcgagctgtg
      541 ccggatgctc aagtccaact acgagaccgc ccacgtcccg gtggtgctgt tctccgcggt
      601 gccgcagcac gagctcgagg gcatggcgcg cgactgcgag gccgacggct gcctgtccaa
      661 gagcaacggg ctcgagcggc tgcccaagga gctgcagctg ctgatcgaga ccgcgctgtt
      721 ctgagaaagc cgggaacgtc ctcgcgaaga cgtcgaggtt gacgcagacg ttgtcgcaac
      781 gcgttgccct tgacggcgac gtgaacgtcg actccaccgt cgacgttgaa cgtggacccg
      841 gcaatcccgg cgagaatcct cgtcgagggc caccgaggag ctcaacgtcg aggtcgacgg
      901 tggtgtctac gtccaacgtc gccgtcgacc tcgacgttcg ggtcaaggtc caggtcatcg
      961 tcaaggtcat gacgggcggc agcgccctcc atctacaccc ccttgattgc agcgcggcgc
     1021 ggcgccgcta ctccggccgg ccccacatga cgcggaacag gcaggcgtcg tcgtcgcgcg
     1081 cctcgcacgc ctcgtgatcg accgcgacgc cgcggccgcc ggacagcacg acgagctgct
     1141 ccagcatgcc ggtggtccac atgcacagct cgggattgcg cggcgtgccg gtgatccgga
     1201 ccaccgtctc gttggggtgg atcggcatgc tggagaccgt gccccagctc tggtagcggc
     1261 tccacacgtt gcggatcgcc gacagcgtcc gctcggggac cagggtcgcg gcgctcgccg
     1321 ggaagaaccg gcggaacgac gcccgcaccg tcgcgcgcgc gatgtcgtgg gcgagccggg
     1381 cgctgtcgcg ctggacgtgc tcgggggcga tggtgagcag ccggatgaac agctcggtcg
     1441 gcagccaggc cagcggcgcg gtgtcggtca gcgcgcgcgc cagctcggtg tgctcgccgc
     1501 cgatcgcgtc gcgcagccgc tcggcctcgc gcacgccgag cgcgcgggcg accgaccgga
     1561 acaccacgcc gcgggtcttg ggcaccgggc tgccgctcct cggcagcacc gtgttggcga
     1621 cccagcgccc gccgtcgggc ttgggcagcg gatcgacccc gatcagggcg gcgcgcagcg
     1681 cgcgcgcgaa cgccccggcg ctgatcgggc gctcgcccgg atcgcggctc atgccgcggg
     1741 tcagcacctc gtcggcgggc tcgagctcgg cgcggatcac cgacggcggc accaggtcct
     1801 cgccgtcgca ctgccgctgc agcacgccct cgccctcgcc ccacggtggg gtcagggtca
     1861 acagcgtgta ggcggtggcc gccaggccgt agacgtcgga ccgcggcgtg gcctcgaagc
     1921 cctcgatcac ctcgggcgcg acgtagcccg gggtgcccgc cgactcggcg aactggccgt
     1981 agcgccgggc gatgccgacg tcgagcagca cgctgcggct gcggaacggg tcgcggatca
     2041 cgttggccgg cttgacgtcg cggtggatga tgccgcgctc gtgcagcgcg tcgagcgccg
     2101 aggcgacctg gtcgaccacg gtcaccagct cgccgatcgg catcagcgtg ccctcgatcc
     2161 ggtggcgctc caccgcctgc tggagtccct cgccctcgag cagctccatc acgaagtagg
     2221 agtcgccgcc ggactggccg aagttgtaga tctgcaccag gttggggtgc tcgagccgcg
     2281 cgagcatggc ggcctcgccg cgcaggtgct cgacgaacgc cttgtcctcg gcgaggtcgg
     2341 ggcgcagcat cttgatcgcg accgggcgct cgagcgcgag gtcctcggcg cggtacacca
     2401 cgcccatgcc gccgcggctg atctcgtgga gcagccggta ggtgccgccc agcgtggcgc
     2461 gcatgtcctc gccgaggtcg atctgctggg tcgacagccg cggcgccgga ccggtcgacg
     2521 cccgcgcgat cgggcgatcg ctgccctgcg ccttcttggc cgcgtgggcg gcgcgggtca
     2581 ccagcatcgc cgcctcctcg ttcttctgca gcgcggcgag cccgaccgtg gccgggatct
     2641 gcagggtgcc gccggtcggc agctcgagcg gccggtcggc gatcgcggcg cggaaccgct
     2701 cggcgacgcg ctgcgagccg tcgatcgggg tgccgggcag caggacggcg atcttgtcgc
     2761 ccgaccaccg gccgacgacg tcctcggtcc ggacgttggc gcggatcgcg tcggcgaccc
     2821 ggcacagcac gcgatcgccg acggccaggc cgtggcgggt gttgatctcg cggagatcga
     2881 ggatgtcgac caggaccgcg gtcagcggca ggccggagcg gtgcgacgcc gagacgtgca
     2941 tggtcgcgag ctgggcggtc gcggtgcggt tccagatccc gagcagctga tcgaggcccg
     3001 ggccgttgac cacgcggtcg agctcgtcgg tggtgcgctg gtggaccgcg cgccagccga
     3061 gctcggcgcc gagccgcgac gcgatcgact gcagcacggt gcggtggtcg cggctgaaga
     3121 tccgcggccg cgtgatgatc aggccgacga agccgtggct gcccagcggg gtgtcgagcg
     3181 gcaccgcgag gtagctctcg cacgcgccgc ccttgaccgg cgacggcgcg atcaggctgg
     3241 cgccggtcgc ggtcgcgatc cggcagtagt cggcggtctg gttccaggtc agcgaatccc
     3301 aggtgtgcga gccgaccgcc gcgccggtcg agtcgccgcg caccgagatc acgcagcgat
     3361 cgacgccgaa cccgaccgcg agctgcgccg ccgcggcctc gagcgcgtcg tcgagcgggc
     3421 ccgacagcac cgagatcgtc agcaggcgat cgagcgcgag cggccggcgc tcggcgagcg
     3481 cgtggtcggt cagcgccagg gcgtggcgca gcgcgggctc gaccacgatg acgtcggcgc
     3541 cgggcgccgg ctcctgcgag gtgtcgcgga tgacctggaa cagcgtggtg cgctcgcggt
     3601 cgcgcagctc ggggacctcg accaggtcgg tcagccggcg cagcacgtcg tcggcctcgc
     3661 cgtactcgcg atcgatgatc accgcgcgcg ggtggctggc cgcgatcgtc gcgaccagct
     3721 cgtcggcgtc gcgcgcgacg aggtacagct gcccggcggt ggcgccggcg ttgacgacct
     3781 cgccccgcac gaggggatcg cgagacagta cgacgaatcc gctattcatg acggtggcgc
     3841 acgctccccg cgccggcatt ttcccgacga agcgtcggta tgtctcgcaa ggagttcagc
     3901 agcatctccc ggcagaatca atggtcgtga agctcggcgg aacggatcgc aatcgctgcg
     3961 cgtcggcgcg cgcgggtcgc gcgtcggggc tgcgcgttca tccgccgcgc gagtcgctgc
     4021 tccgcgtaca ccgcgcggtg cgaaacgccg cgggccgccg ggtccacggt cgagtcgcag
     4081 ccctccgcga tcaagcgtaa gaaccgttac accatcaagc gcgcctcgcg atcgcccgga
     4141 caggagatca cgcgtcgact ccattccggt gcaggtcgtg cagcgttttg cggcatcgat
     4201 gttttgcgct cccgtcggag ccccgcggta cattcgacgc acgtcgacga tggctgaagc
     4261 acgttcgctg cagaatgtcc tggtggtgga tgacgacgag cgaatcctcg gttcctggaa
     4321 acgagcggct cgcgagcgca acgtgttcac agcgatcgat gctgcgaccg cgcggcagct
     4381 ggcgagtacg gagaagccgg atctcgcgat cgtcgacctg aggctgggca acacctccgg
     4441 catcgatctg attcgcgacc tcaagcgcga tctccccgat ctgctggtcg tgctgtgcag
     4501 cggctacctg tcggtcgccg ccgcggtggc cgcggtccgc gccggggcgg atatcgtggt
     4561 gttcaagccg atcacgtttc gcgagatcct gcagcggatc gagcagaact ccgaggagcc
     4621 cgatctggaa gacactccga cgctggcccg tgcggagtgg gagcacatca tgcgcgtgct
     4681 cgcggactgc aacggcaatg tctcgatggc ggcgcgccgg ctcgggatct accgcagctc
     4741 gctgcagcgt cgactccgca agtacgcccc acgctcgtaa cccgctgtcg atctccgccg
     4801 gcgatctgag ccggccgggg ccggacagga aaggggaggg gagggtgaga tggcaggaat
     4861 gaataccgtg tagtcgttgg gtccagcaga ctgcggtccg tcgtttccgc gttccgctgc
     4921 gatggccagg ttttcgatga gcgattgtcc gatcttgtgt caccatgcgc cgggtggccg
     4981 gtgggtcggg ggtggccggc gagcggcacg accagcaccg gcatccacac ctaccgggtg
     5041 gcggcaatgg aaatgatggc ggcaatcgcg cgcttcgatg agaaggtgag ccatggcggc
     5101 agccaatagc gagcggttca ttgtccgcgc cgaggtccgc ttgggggacg acaccgtgat
     5161 ctcgtacggg ttcgacttga cctcgagctc ggtcttcgtc gtgaccgaat ggcacgcgcc
     5221 gatcggtacc ccggtggcgc tccggctgtc gttcccgcgg atcctcgagc cgatcgacgt
     5281 cgtcgcccgg gtcgccgaca tccgggtcgc gggggagccc ggcgagccgg gcggcgtccg
     5341 gctggcgttc gacgagggcg aggtcggggc ccggctgatg tcgcagctcg agcacacggg
     5401 tggcgccgac cccgccgcgg accgcccgta ccgcgtgctg ctggtcgacg acagctggct
     5461 gatccgcgac atgttcgccc acggcaccgc gaagttcttc gaccggcccg gcacgctcgc
     5521 gatcgacggc gccgagaacg ccgaggaggc gtggagcaag ctggcgaagt cgacctacga
     5581 tctgctgatc gtcgactact tcctgcccgc cgaggacggc gcctcgctga tcgcccggct
     5641 gcgcgccgat gcccggctcg cccggactcc gatcgtcgcg atcagcgtgg gcggtcgcga
     5701 cgcccgcgag gcgacgatct cggccggcgc cgacgtgttc gtcgacaagc cgctggtgtt
     5761 ccgcgacctg ttcaacacgc tgcgcgtgct cgccgagcac cgcgagcggt cgcggccgcc
     5821 ggcggagcgc aaggcgatcc tggtgttcga cgacagcccg ttcgcgctcg cgctgacccg
     5881 cgccgcgctc gaggccgccg ggttcaccgt cgcgatcgcc gccgatctgt cgtcgttcga
     5941 gcgccagcgc gtcgcgttcg atccc
//
LOCUS       DASVOR010000450         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_296547,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000450 DASVOR010000000
VERSION     DASVOR010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296547"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="VF516_08925"
     CDS             <1..283
                     /locus_tag="VF516_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107842.1"
                     /translation="LGQAERAGVGLERAPSDLGVALGIAEPAAAPAAEHIAVFIFLLI
                     LAAARAERALLATAAVAAELAAPAITPAVVFVTAAAVVSIFPRHFSVSL"
     gene            complement(304..1020)
                     /locus_tag="VF516_08930"
     CDS             complement(304..1020)
                     /locus_tag="VF516_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8107843.1"
                     /translation="MKSQLEISTKSGTNQTGLAPHGDVLAKMIQGANEFPPTSSGGPA
                     GIAEVRVDYARDGEPIATMPSAPVTPAKLPLLDRLGARMQFERTGTRLYDALISKLDA
                     YGSFDGGPSRQELLEIREQELSHALLTQELVSSMGGDPTAITPDANLQAMASQGIACV
                     LLDPRTSLVDCLEAMLIIELTDQDMWGMLARTAEPLGEAAMIDKITQAQKVELEHLAK
                     VRGWLEAADQARSKVARSTR"
     gene            complement(1017..>1500)
                     /locus_tag="VF516_08935"
     CDS             complement(1017..>1500)
                     /locus_tag="VF516_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107844.1"
                     /translation="ALEKWLLHEATEAYNYWSCLEHEDDARIRKIWERMLEYELGHVQ
                     AVARLFEDHERRDAQEVITRRIRRPITYVSHREFVRGVLRSEVNLGADGIHLIDRTRD
                     HEPERTRRYRAELNREIQPSNVVAAGWVWQPGGELASRETGKHPDSNGVKSPSQGGKS
                     "
BASE COUNT          180 a          536 c          513 g          271 t
ORIGIN      
        1 cctcgggcaa gccgaacgcg cgggcgtggg cctcgaacgt gcgccgtccg acctcggcgt
       61 ggcgctcggg atcgccgaac cagccgccgc gccggcggcc gagcacatcg cggtcttcat
      121 cttcctcttg atcctcgcgg ccgcgcgagc ggaacgagcg ctcctcgcca cggccgccgt
      181 agccgccgaa ctggctgctc cggctatcac gccagcggtc gtcttcgtca cggctgctgc
      241 ggtagtctcg atcttccctc ggcatttcag tgtctccttg tagtttctcg atgtggtagt
      301 ggactagcgc gtgctgcgcg cgaccttgct gcgcgcctgg tcggcagcct ccagccagcc
      361 gcgcaccttc gcgaggtgct cgagctcgac cttctgcgcc tgtgtgatct tgtcgatcat
      421 ggcggcttcg ccgagcggct cggccgtgcg ggcgagcatg ccccacatgt cctgatcggt
      481 gagctcgatg atcagcatcg cctcgaggca gtcgaccagg ctggtccgcg gatcgagcag
      541 cacgcacgcg atgccctggc tcgccatcgc ctgcaggttc gcgtccggcg tgatcgcggt
      601 cggatcaccg cccatcgacg acaccagctc ctgggtcagc aaggcgtggc tgagctcctg
      661 ctcgcggatc tccagcagct cttgccggct cgggccgccg tcgaagctgc cgtaggcgtc
      721 gagcttggag atcagcgcgt cgtacagccg ggtcccggtg cgctcgaact gcatccgcgc
      781 gccgagccgg tcgagcagcg gcagcttggc gggtgtcacc ggcgcgctcg gcatcgtcgc
      841 gatgggctcg ccgtcccgcg cgtagtcgac gcggacctcg gcgatcccgg cgggaccacc
      901 cgacgaggtc ggcgggaact cgttggcgcc ctggatcatc ttggcgagca cgtcgccgtg
      961 gggcgccagc ccggtctggt tggtccccga cttggtcgag atctcgagct gtgacttcac
     1021 gacttgcctc cctggctcgg cgacttgacg ccgttggaat cggggtgctt gccggtctcg
     1081 cggctcgcga gctcgccgcc cggctgccag acccagcccg ccgcgaccac gttcgacggc
     1141 tggatctcgc ggttgagctc ggcgcggtag cgccgcgtcc gctccggctc gtggtcgcgc
     1201 gtgcgatcga tcaggtggat gccgtcggcg ccgaggttga cctccgagcg cagcacgccg
     1261 cgcacgaact cgcggtggct gacgtaggtg atcggccggc ggatccggcg cgtgatcacc
     1321 tcctgcgcgt cgcgccgctc gtggtcctcg aacagccggg ccaccgcctg gacgtggccg
     1381 agctcgtact cgagcatgcg ctcccagatc ttgcggatcc gcgcgtcgtc ctcgtgctcg
     1441 aggcagctcc agtagttgta ggcctcggtg gcctcgtgca gcagccactt ctcgagcgcc
//
LOCUS       DASVOR010000451         2043 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501086,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000451 DASVOR010000000
VERSION     DASVOR010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501086"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2043
                     /locus_tag="VF516_08940"
     CDS             <1..>2043
                     /locus_tag="VF516_08940"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEX8107845.1"
                     /translation="VEGHALAGVELAGYLIGQPLGAAPVVIVVGGITASPFPFGDPAS
                     GTEAWWPALQAPDLVDLSRTTVLCPCWPGNGSTWRGFDDGAVPTLSALGLADLIAAWL
                     DGLGCTAPVTYVGASLGGLVGIAFAVRHAERCARLITISAGLRPDGWGTATRHLQREL
                     VRDGLRRGDVATGMIRARQLGMLTYRGREELDTRFGVLVPELDRPPVAAYLDHHGERF
                     AARFPVHTFLLLSEAIDRSHFGTDRASLRAQLARVTADVLVVGVPGDLVFPFALQHEL
                     YRELQAVGAACSLWKLDSEYGHDAFLADQDRLAALLRDASAFGEAAPRARFEGIGARP
                     VREIKIGMVGCGTVGTGVLELLDRQAGAMAERYGVRFRVTRIAVRDPARPRSRLADGI
                     ARTATALELVADPEVDVIVEVAGGLAVEATVAAALAAGKPVVTANKALLARKLAALGV
                     LAQRTGTPLLCEAAAAAALPIIRHLSHRADEIDSLMAIVNGTCNYIITRLEQDEWALD
                     RAIAEAQRLGLAEADPSADLDGLDAAAKLSILAYRAFGAWLPPDTLPVRGIGELWPAD
                     CDLAEAMGFRIRLIAHAARRGAALTAAVEPVLLPDWHLLASVEEEYNAVYLKCATAGD
                     LSLFGKGAGGLPTATAILGDLIDLAQDNSVQWPVPHRGRPAPSPPRRHYLRVSAEA"
BASE COUNT          229 a          752 c          782 g          280 t
ORIGIN      
        1 ggtcgagggg cacgccctgg ccggggtcga gctcgccggg tacctgatcg gtcagccgct
       61 cggcgccgcg ccggtggtga tcgtcgtggg cgggatcacg gcctcgccgt ttccgttcgg
      121 cgatccggcg agcggtaccg aggcgtggtg gcctgcgctc caggcgcccg acctggtgga
      181 cctgagccgc acgacggtgc tgtgcccgtg ctggccgggc aacggctcga cctggagggg
      241 attcgacgat ggcgcggtcc cgacgctgtc cgcgctcggc ctcgccgacc tgatcgcggc
      301 ctggctcgat ggtctgggct gcacggcccc ggtgacctac gtcggggcga gcctgggtgg
      361 gctggtcggc atcgcgttcg cggtccgcca cgccgagcgc tgcgcgcggc tgatcacgat
      421 ctcggccggg ctgcggcccg acggctgggg caccgcgacg cgccacctcc agcgcgagct
      481 ggtgcgcgac gggctgcgcc gcggcgacgt cgcgaccggg atgatccggg cgcgccagct
      541 cggcatgctg acctaccgcg gccgcgagga gctcgacacc cggttcggcg tgctggtgcc
      601 cgagctcgat cgcccgccgg tcgcggcgta cctcgatcac cacggcgagc ggttcgccgc
      661 gcgattcccg gtccacacgt tcctgctgct gtccgaggcg atcgatcgca gccacttcgg
      721 gaccgatcgc gcgtcgctgc gcgcccagct cgcgcgggtg accgccgacg tcctggtggt
      781 cggcgtgccg ggcgatctgg tgttcccgtt cgcgctgcag cacgagctgt accgcgagct
      841 ccaggcggtg ggcgcggcgt gctcgctgtg gaagctcgac tcggagtacg ggcacgacgc
      901 gttcctcgcc gatcaggacc ggctcgccgc gctgctgcgc gacgccagcg cgttcgggga
      961 ggccgcgccg cgcgcccggt tcgagggcat cggcgcccgg ccggtccgcg agatcaagat
     1021 cgggatggtg ggctgcggga cggtcggcac cggcgtgctc gagctgctcg atcgccaggc
     1081 cggcgcgatg gccgagcgct acggcgtgcg gttccgggtc acccggatcg ccgtccgcga
     1141 tcctgccagg ccgcgcagcc gcctggcaga cggcatcgcc cgcacggcca ccgccctgga
     1201 gctggtggcc gaccccgagg tcgacgtgat cgtcgaggtc gccggcggac tggcggtcga
     1261 ggccacggtc gccgcggcgc tcgccgccgg caagccggtg gtcaccgcga acaaggcgct
     1321 gctcgcgcgc aagctcgccg cgctcggcgt gctcgcccag cgcaccggga cgccgctgct
     1381 gtgcgaggcg gcggccgccg cggcgctgcc gatcatccgc cacctgtcgc accgcgccga
     1441 cgagatcgac agcctgatgg cgatcgtcaa cgggacctgc aactacatca tcacgcggct
     1501 cgagcaggac gagtgggcgc tcgatcgcgc gatcgccgag gcccagcggc tcgggctcgc
     1561 cgaggccgac ccgtcggccg acctcgacgg gctcgacgcg gcggccaagc tgtcgatcct
     1621 cgcgtaccgc gcgttcggcg cctggctgcc gcccgacacc ctgccggtcc gcgggatcgg
     1681 cgagctgtgg ccggccgact gcgatctcgc cgaggcgatg gggttccgga tccggctgat
     1741 cgcgcacgcg gcgcggcgcg gcgcggcgct gaccgcggcg gtcgagccgg tgctgctgcc
     1801 cgactggcac ctgctggcgt cggtcgagga ggagtacaac gcggtctatc tcaagtgcgc
     1861 gacggcgggc gacctgtcgc tgttcggcaa gggcgccggc gggctgccga ccgcgaccgc
     1921 gatcctcggc gacctgatcg acctcgcgca ggacaactcg gtgcagtggc cggtgccgca
     1981 ccgcggccgg cccgcgccgt cgccgccgcg ccggcactat ctgcgggtca gcgccgaggc
     2041 cca
//
LOCUS       DASVOR010000452         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_559672,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000452 DASVOR010000000
VERSION     DASVOR010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559672"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VF516_08945"
     CDS             <1..239
                     /locus_tag="VF516_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107846.1"
                     /translation="AFVGTAVLAQLVITLSRAGIIPPGTGEQIALLGITTFKLTITYY
                     ISVLQERTFHVYEYYGGQVRASSRIPASAAWACA"
     gene            complement(176..352)
                     /locus_tag="VF516_08950"
     CDS             complement(176..352)
                     /locus_tag="VF516_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8107847.1"
                     /translation="MERRQIRVLLEEAEVQRRLGNHRGATELAQRALTIDPDHAQAHA
                     ALAGILLDARTWPP"
     gene            complement(352..858)
                     /locus_tag="VF516_08955"
     CDS             complement(352..858)
                     /locus_tag="VF516_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107848.1"
                     /translation="MSYQIADEPVETSLRSYVVRPSAPLLAAMLAGAWLAWPWFAFNA
                     IAMGSPTRRKEIALCAAAFAGTAVLGAIVIALFDAGLLPPGVPLRLAVLGVVAFKLAI
                     TYHISAVQSRTFHVYEYYGGPVRGAGRILGAAILLRGFVIGLVDHPLWIIIVASGVPG
                     AHAGLWGW"
     gene            complement(890..>1948)
                     /locus_tag="VF516_08960"
     CDS             complement(890..>1948)
                     /locus_tag="VF516_08960"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8107849.1"
                     /translation="DQAAALAAYEAAVRANPTLEDRDLRALLAAQVRVHRGDGDGPRL
                     RVVSNDDTDHSEVDRLLHPAAPPITFAEVGGLDEIKAQIEKRIILPFHKPALFARFKR
                     KPGGGILLYGPPGCGKTLLARATAGQCQARFFHVAIEDVLDMWIGESERKLHALFEQA
                     RAATPAVMFFDELEALAGKREYTREATSSKLVSQFLSEMDGFVKNNTGVLILAATNVP
                     WAIDPAFRRPGRFDRVLFVPPPDRTARAAILDILLAGRPTTGDVDPAALAARTSGFSG
                     ADLENLVDTAADRAIAASLETGAEVPIDQRHLVGALGEIKPTTLEWLTTARNYARYAN
                     EGGQYDEVLEFLRRHGKS"
BASE COUNT          272 a          730 c          675 g          271 t
ORIGIN      
        1 cggcgttcgt cggcacggcc gtgcttgccc agctcgtgat cacgctgtcg cgcgccggca
       61 tcatcccgcc gggcaccggc gagcagatcg cgctgctcgg catcacgacg ttcaagctga
      121 cgatcaccta ctacatctcg gtgctgcagg agcgcacgtt ccacgtctac gagtactacg
      181 gcggccaggt ccgcgcgtcg agcaggatcc cggccagcgc cgcgtgggcc tgcgcgtgat
      241 cggggtcgat cgtgagcgcg cgctgcgcga gctcggtcgc gccgcggtgg ttgccgagcc
      301 ggcgctggac ctcggcctcc tcgagcagca cgcggatctg gcggcgctcc atcaccaccc
      361 ccacagcccg gcgtgcgcgc cgggcacccc gcttgcgacg atgatgatcc acagcgggtg
      421 atcgacgagc ccgatcacga acccgcgcag caggatcgcc gcgccgagga tccggccggc
      481 gccgcgcacc gggccaccgt agtactcgta gacgtggaac gtccggctct gcaccgcgga
      541 gatgtgatag gtgatcgcga gcttgaacgc gaccacgccg agcaccgcga gccgcagcgg
      601 cacgcccggg ggcaacaggc cggcatcgaa cagcgcgatg acgatcgcgc cgagcacggc
      661 cgtgccggcg aacgccgccg cgcacagcgc gatctccttg cgccgcgtcg ggctgcccat
      721 cgcgatcgcg ttgaacgcga accacggcca cgccagccac gcgcccgcga gcatggccgc
      781 gagcagcggc gcgctcggcc gcaccacgta gctgcgcagc gaggtctcga ccggctcgtc
      841 ggcgatctgg tagctcatcg caccccctgc gcggtgttcc cgggcccggt catgacttgc
      901 cgtggcggcg gaggaactcc agcacctcgt cgtactggcc gccctcgttg gcgtagcgcg
      961 cgtagttgcg cgcggtggtc agccactcga gagtcgtcgg cttgatctcg ccgagcgcgc
     1021 cgaccaggtg gcgctgatcg atcggcacct cggccccggt ctcgagcgac gccgcgatcg
     1081 cgcggtccgc ggcggtgtcg accaggttct cgagatccgc ccccgagaac cccgaggtcc
     1141 gcgcggcgag cgccgccgga tcgacgtcgc ccgtggtcgg ccggccggcg agcaggatgt
     1201 cgaggatcgc cgcgcgcgcg gtgcggtccg gcggcggcac gaacagcacc cggtcgaacc
     1261 gacccggccg gcggaacgcc ggatcgatcg cccacggcac gttggtcgcc gccaggatca
     1321 gcacgcccgt gttgttcttg acgaagccgt ccatctcgga caggaactgg ctgaccagct
     1381 tggacgacgt cgcctcgcgg gtgtactcgc gcttgccggc gagcgcctcc agctcgtcga
     1441 agaacatcac cgccggcgtc gcggcgcgcg cctgctcgaa cagcgcgtgc agcttgcgct
     1501 cgctctcgcc gatccacatg tcgagcacgt cctcgatcgc gacgtggaag aaccgcgcct
     1561 ggcactgccc cgcggtcgcg cgcgccagga gcgtcttgcc gcagcccggt ggaccgtaca
     1621 gcaggatgcc gccgcccggc ttgcgcttga acctcgcgaa cagcgccggc ttgtggaacg
     1681 gcaggatgat ccgcttctcg atctgcgcct tgatctcgtc gagcccgccg acctcggcga
     1741 acgtgatcgg cggcgcggcc ggatgcagca gccggtcgac ctcgctgtga tcggtgtcgt
     1801 cgttggacac cacgcgcagc cgcggcccgt cgccatcgcc gcggtgcacc cggacctgtg
     1861 ccgcgagcag cgcgcgcagg tcgcggtcct ccagcgtcgg gttggcgcgc accgccgcct
     1921 cgtacgccgc cagcgccgcc gcctggtc
//
LOCUS       DASVOR010000453         7739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208773,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000453 DASVOR010000000
VERSION     DASVOR010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7739
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208773"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="VF516_08965"
     CDS             <1..1211
                     /locus_tag="VF516_08965"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8107850.1"
                     /translation="SAPPEECLARGGAGAALDFDFRTNNSEYGVLGQVDTSRVIAGPP
                     ERILPDGSALRPGTTGYGGYVRAGRFGGEGFRWEIADDFSTPTLDLNATGFQRTQNEH
                     APKVTLRYEEPEGVGPLKGLSARLLGQGRWTTDGRSLNRCNTLDSALSITLPSFDTVG
                     LEWEDDAGGCYDVRELRGTGVPLEQDSINTFALFFDTNASRILSASGFAALERFGRGP
                     TPPAWGGTVNVALSLRPRPALETGLEVILDHTELPPRFLGRMPDGSFLLGNLASSFLS
                     LTLRQQWLIRPRFTLQGYTQLFVNHAAYGPFFTAASSDARAPVLFSAMQPTTSSERRI
                     YGAALNLNLVLVWEYRLGSTLSFVITHSQQGLPPPDGELPPATLVPRQLFPGPATDAV
                     LLKWNYFWAL"
     gene            1267..2058
                     /locus_tag="VF516_08970"
     CDS             1267..2058
                     /locus_tag="VF516_08970"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8107851.1"
                     /translation="MTQPYLRLRQIALVARALEPVERQLTSVLATEVCFRDPGVGKYG
                     LHNALWALGGTFLEVVAPIRDNTAGGRYLDRRGGDGGYMYIVDCDDLDARRAQLGALG
                     VRLVADVRAGDAVLWSEAIHLHPKDTGGCLLSIDRHSGGADTMGGYRWAGADWHSKAR
                     RDVVISGAVMQCDDPAATARRWAHLLGRPATASADGGHELQLDNAVARFVPLADDRGE
                     GLAGVMLSCAEPASMIAAATAIGASVGSSWFEVCGVRFALENRTA"
     gene            complement(2084..4054)
                     /locus_tag="VF516_08975"
     CDS             complement(2084..4054)
                     /locus_tag="VF516_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107852.1"
                     /translation="MRELSWRCDRRMAGAPAILRRCALLVAVALGCGAAACGDSRHPA
                     TDAGVPADAPAPDAAPPDAASPDAACACTTTARCLDATTLETSLPRGACVAGACSYDA
                     VTSPCPFGCKDGACQAEACVPSCPAAICGDDGCGGSCGACDPGTTLPGVTPVELGTAG
                     DLFVAPDGIHLATMRAFEPRCDLFPSDVGRLDVWTVPVTGAPSHRTIGARVSGISGFG
                     VTWTSGGDLLYLDRSPPCSSPSELWIAHADGSQPRRLASGVLSFRTAGAVVVYESLPD
                     LTLYAIQLPDGRPIPLTSYTRDNLLGFYELSPDGTAVWAISTPAQLFDPRDVRLFRTD
                     GSLSRRLVTGTDSPIGLPIWAPDSKHLAYQTGSIGGAVVDRDGNGRVILTQHPGGEPF
                     QQFAVFSADGHRLAFIEQPVQFGLDVVIHSFDGRPEVRIGSVVSPAVGGLIFKLAFSA
                     DGARIYATTAADVDAPSDVMTGRTDVSGTAQLFAAGASRWSETRDGSVVAAEGINTQV
                     ITLGGGTQTIAGLPFELARFEPVATNPRLLVQSGDFLSVYPTSGAGPGIPLPLFFWTP
                     ELSNWAGGHNAPFPFGWAGSMALYPADTRGVALDLVSFDLLAWTPSASGRLLSRVARY
                     QIVDQPSRIYAMTDSGRLFMIPRPAASSTLAR"
     gene            4137..6371
                     /locus_tag="VF516_08980"
     CDS             4137..6371
                     /locus_tag="VF516_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX8107853.1"
                     /translation="MRNSSYVYALALLAACGGPPRAAPDAALPPDAALPADAAVDGDA
                     GSGGIPACVPSGWVPWPAANDLPIATIVADVNGDGKPDLVTATPDVFVPGAVSVFLAS
                     GNGTFQARTDYRVDVAPTSLAVADVSGDGKPDILVTSEPTRAMSVMLGRGDGTFQDAV
                     DYPTGRSPTGLVIADVTGDGKPDAVLIDGSTVSVLRGNGDGTFQARVDLSLAARPTMI
                     ALADLSGDHKPDLIFTRRSDQTVGVLVGNGDGTFRPEVDDPTGGSPSGLAIAEVTGDG
                     KLDVVVTSDDFGIVSVLAGNGDGTLKPRIDATTGAALGLVAIADLTGDGKPDLAVIDQ
                     SHRALSVLAGNGDGSFGARTDTPVGVSPFGVAVADVSGDGKLDVVVSNTSGVLSILVG
                     NGDGTFQTQPHYATGTTPGGVAAADVNADGKPDLLVASTGANTLSVLLGTGGGTFGAA
                     ADTPTGQAPAGIAVADVNGDGRPDAILTNQTDATVSVLLGSSNGTLRPRVDYPTNVFP
                     GVPIVADLNGDGKPDLVIPYNVGFTNNTVSVLLGNGDGTFQPKIDSLSGPATSAATVA
                     DLNHDGKLDLIASSQGEGTVSVLLGNGDGTFQPKVDHFLRFTAVVTAMAAVDLDRDGA
                     LDLVMAEDNFGRVLVLFGNGDGTFQPETDYPAGQKPVSLVIADIDGDGQVDLAVADFT
                     GNTVNVLRGNRDGTFRPQIVYPAGKTPISLAVADVDHDGRPDLVVANRDPATVTVLRA
                     RCLR"
     gene            complement(6417..>7739)
                     /locus_tag="VF516_08985"
     CDS             complement(6417..>7739)
                     /locus_tag="VF516_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107854.1"
                     /translation="VLIEEERHARLIGDVEQRVTCIGALTRTLPPAAREPVVSRMLAA
                     IADGTGGRRSGRSLAALAPVAAAGQLDRMADALSTVQGLERARAAAALFAANARLGRW
                     DRALGFLPELDNGHHLRLALDQLPEEVPAEVVRTILRGAADTADAELGATAIAPLARR
                     LPEDEREAACELGLQAAEVLRLPDRYRLLEALIPAIPCERLDAASALISDDDHNALME
                     RDGALVALAVRRGDCGDLPTAISILDRMTYVDARCRACVQWCGRTGLPEEIRFRFLEW
                     AETFADGESNYMRVELLAEVARQHTEPHRSRLLEKALALARAIKKSSKSFVVSANVDT
                     ALALASLAPLVDASRQADIMLEAWDRAADAGTERARGLEAVAPLVATLGPDRIAARWR
                     NQLERVLGERRDVLAELEVMAPVIERLGGEDGVLGVIAAIETTARWWP"
BASE COUNT         1186 a         2781 c         2696 g         1076 t
ORIGIN      
        1 agagcgcgcc gcccgaggag tgcctggcgc gaggcggtgc cggggcagcg ctcgacttcg
       61 atttcaggac gaacaactcc gaatacggcg tgctcgggca ggtcgacacc agccgcgtca
      121 tcgcgggccc ccccgagcgc atcctgccgg acggctcggc gctgcggccc ggcaccaccg
      181 gctacggtgg ctacgtgcgc gccggcaggt tcggcgggga gggctttcgc tgggagatcg
      241 ccgacgactt ctccacgccg accctcgatc tgaacgccac cgggtttcag cgcacgcaga
      301 acgagcacgc gcccaaggtc accctgcgct acgaggagcc cgagggggtg ggaccgctca
      361 agggcctgtc cgcccggctg ctggggcaag gccgatggac caccgacgga cgaagcctga
      421 accggtgcaa caccctggac agcgcactct cgatcaccct gcccagcttc gataccgtgg
      481 gcctcgagtg ggaggacgac gcgggcggct gctatgatgt ccgcgagctc aggggcaccg
      541 gcgtacccct ggagcaggac tccatcaaca ccttcgcctt gttcttcgac acgaacgcaa
      601 gccggatcct ctcggccagc ggcttcgccg cgctggagcg cttcggccgg ggcccgacgc
      661 caccggcctg gggcggcacc gtgaacgtcg cgctgtcgct gcggccgcgg ccggcgctgg
      721 agaccggcct ggaggtgatc ctcgatcaca ccgagctccc cccgcggttc ctgggtcgga
      781 tgcccgacgg cagcttcctg ctcgggaacc tcgcgtcctc gtttctctcg ctcacgctgc
      841 gtcagcagtg gctgattcgc cccaggttca cgctgcaggg ctacacccag ctgttcgtga
      901 accacgccgc gtacggcccg ttcttcaccg cggcgtccag cgacgcgcgc gcgccggtcc
      961 tgttctccgc gatgcagccc accacgtcca gcgagcgccg gatctacggc gcggccctca
     1021 acctcaacct cgtgctggtc tgggagtacc ggctgggctc cacgctgtcg ttcgtgatca
     1081 cgcactcgca acagggcctg ccgccgcccg acggagagct cccgcccgcg acgctggtac
     1141 ctcggcagct gttcccgggc cccgccaccg acgcggtgct gctgaaatgg aactacttct
     1201 gggccctgtg atccgcggtc gcgcttgtca ccgttccgcg cccgctgcat gctcgccggc
     1261 gaaccgatga cacaaccgta tctccgcctg cgccagatcg cgctcgtggc gcgcgcgctc
     1321 gagccggtcg agcggcagct gacgtccgtg ctcgcgaccg aggtctgctt ccgcgatccc
     1381 ggcgtgggca agtacggcct gcacaacgcg ctctgggcgc tgggcggaac cttcctggag
     1441 gtcgtcgcgc cgatccggga caacaccgca ggcggccggt atctcgatcg ccgcggcggc
     1501 gacggcggct acatgtatat cgtcgattgc gacgacctcg acgcgcgacg cgcgcagctc
     1561 ggcgcgctgg gggttcgcct cgtggcggac gtccgcgccg gcgatgcggt gctgtggtcc
     1621 gaggcgatcc acctccaccc caaggacacc gggggctgcc tgctctccat cgaccgccac
     1681 tccggcgggg cggacacgat gggaggctac cggtgggcgg gggcggactg gcactcgaag
     1741 gcgcggcgcg acgtggtgat ctcgggcgcc gtcatgcagt gcgacgatcc cgccgccacg
     1801 gcccggcgct gggcacacct cctgggccga cccgcgacgg cgagcgccga cggcggccac
     1861 gagctgcagc tcgacaacgc ggtcgcccgc ttcgtcccgc tcgccgacga ccggggagaa
     1921 gggctcgcgg gcgtgatgct ctcgtgcgcc gaacccgcgt ccatgatcgc tgccgcgacg
     1981 gcgatcggcg cctcggtcgg atcgagctgg ttcgaggtct gcggcgtgcg gttcgcgctc
     2041 gagaaccgca ctgcgtgatg agcgtccggg tcaacctgcc gcgtcaccgc gcgagcgtcg
     2101 acgaggccgc cgggcgcggg atcatgaaca gccgcccgga gtcggtcatc gcgtagatcc
     2161 gggacggttg gtcgacgatc tggtaccggg cgacccgcga cagcaggcga ccgctggccg
     2221 agggcgtcca cgcgagcagg tcgaagctca ccagatcgag cgccacgccg cgggtgtccg
     2281 cgggatagag cgccatcgag ccggcccagc cgaacgggaa cggcgcattg tgcccgccgg
     2341 cccagttcga gagctcgggg gtccagaaga agagcggaag cgggatcccc gggccggcgc
     2401 ccgaggtcgg atagaccgac aggaagtccc cgctctggac caggaggcgc gggttggtcg
     2461 cgaccggctc gaaccgggcg agctcgaacg gaagaccggc gatcgtctgc gttccaccgc
     2521 cgagcgtgat cacctgggta ttgatgccct ccgccgccac caccgagcca tcccgggtct
     2581 cgctccaccg gctcgcgccg gcggcaaaca gctgcgcggt cccgctgacg tcggtccggc
     2641 cggtcatcac gtcggacggc gcgtccacgt cggccgcggt ggtggcatag atgcgggcgc
     2701 cgtcggccga gaacgccagc ttgaagatca agccgccgac cgcgggcgac acgacgctgc
     2761 cgatccggac ctcggggcgc ccgtcgaacg aatggatcac gacgtcgagc ccgaactgca
     2821 ctggctgctc gatgaacgcc aggcggtggc cgtcggcgga gaacacggcg aattgctgaa
     2881 acggctcccc acctggatgc tgggtcagga tcacccggcc attgccatcg cgatcgacca
     2941 ccgcgccccc gatgctcccc gtctggtacg ccaggtgctt gctgtccggg gcccagatgg
     3001 gcaaaccgat cggcgagtcg gtcccggtga ccagccgccg ggacaagctg ccatcggtcc
     3061 ggaacaaccg gacatcccgg gggtcgaaca gctgcgctgg agtcgatatc gcccagacgg
     3121 ccgtgccatc cggcgacagc tcgtagaagc cgagcaggtt gtcgcgggtg tacgacgtga
     3181 gcgggatcgg ccggccgtcg gggagctgga tcgcatacag cgtgagatcg ggcagcgact
     3241 cgtacaccac gacggccccg gcggtccgga acgaaagcac gccgctcgcc agacggcggg
     3301 gctggctgcc gtcggcatgc gcgatccaga gctcggaagg cgagctgcac gggggtgagc
     3361 ggtcgaggta gagcagatca ccgcccgagg tccacgtgac cccgaaccct gagatgcctg
     3421 atacgcgcgc accgatcgtc cggtgggaag gtgccccggt gaccggcacg gtccagacat
     3481 ccagacgacc gacgtccgaa gggaacaggt cgcagcgcgg ctcgaacgcg cgcatcgtcg
     3541 cgaggtggat gccgtcgggt gcgacgaaca gatcgccggc ggtgccgagc tcgaccggcg
     3601 tcacgccggg cagcgtggtg ccggggtcgc acgcgccgca cgagccgccg cagccgtcgt
     3661 cgccacagat cgcggccggg cagctcggca cgcaggcctc ggcctggcac gcgccgtcct
     3721 tgcagccgaa cgggcacggc gaggtcacgg cgtcgtagct gcacgcgccc gcgacgcacg
     3781 ctccgcgcgg cagcgaggtc tcgagcgtgg tcgcgtcgag gcaacgcgcg gtggtcgtgc
     3841 acgcgcacgc cgcgtcgggt gatgccgcat ccggcggcgc cgcatccggc gccggtgcat
     3901 ctgcggggac cccggcatcg gtcgccggat gtctgctgtc gccgcacgcc gccgctccgc
     3961 atcccagcgc gactgcgacg agcaacgcgc accggcggag gatcgccggc gccccggcca
     4021 tgcggcgatc acacctccac gagagctcgc gcatggctcg ctgttatacc gcacggctcg
     4081 ctcgcgcgag aatccgcacg agcgatatag tccgctgcgg tcccggaaac gtcgtgatgc
     4141 ggaactcctc gtatgtgtac gcgctcgccc tgctcgcagc atgcgggggc cctccccgcg
     4201 cggcccccga cgctgcgctg cctcccgacg ctgcactgcc ggccgacgcc gcggtcgatg
     4261 gcgacgcggg gagcggaggc attccggcct gcgtgccgtc cgggtgggtc ccgtggccgg
     4321 cggccaacga cctgccgatc gcgacgatcg tggcggacgt caacggcgat ggcaaacccg
     4381 atctcgtcac cgccacgcca gacgtatttg ttccgggcgc ggtgagcgtg ttcctggcca
     4441 gtggcaatgg cacgttccag gccaggacgg attatcgagt ggacgtggcg cccacctcgc
     4501 tggcggtggc cgacgtcagc ggggacggca agcccgatat cctggtgacc tccgagccca
     4561 cgcgggcgat gagcgtgatg ctcgggcgcg gcgacgggac gtttcaggac gcggtcgact
     4621 atcccaccgg aaggtctccc accggcctcg tgatcgccga cgtgaccggc gacggcaagc
     4681 ccgatgccgt gctgatcgac ggcagcaccg tgagcgtgct gcggggcaac ggcgacggca
     4741 ccttccaggc cagggtcgac ctctcgctcg cagccagacc gacgatgatc gcgctggcgg
     4801 atctcagcgg cgatcacaag cccgatctca tcttcacccg ccgcagcgac cagacggtgg
     4861 gcgtcctggt gggcaacggc gacggcacgt tccggcccga ggtcgacgat ccgaccgggg
     4921 gaagcccgtc cggtctcgcg atcgcggagg tcaccgggga cggcaagctc gacgtcgtgg
     4981 tgacgagcga tgacttcggc atcgtcagcg tgctcgcggg caacggcgat ggcacgctga
     5041 agccccggat cgacgccacg accggcgcgg ccctgggctt ggtcgcgatc gcagatctca
     5101 ccggcgacgg gaagcccgat ctcgccgtga tcgaccaaag ccaccgggcg ctgagcgtgc
     5161 tcgccggcaa cggcgacggc agcttcggag ccaggacgga tactccggtg ggcgtgtcgc
     5221 ccttcggggt cgcggtggcg gacgtcagcg gagacggcaa gctcgacgtc gtggtcagca
     5281 acacgtcggg ggtgctgagc atcctcgtgg ggaacgggga tggcacgttc cagacccagc
     5341 cgcactacgc aacgggcaca acccccggcg gggtcgcggc ggcggacgtc aacgccgatg
     5401 gcaagcccga cctcctggtg gccagtaccg gagccaacac gctgagcgtg ctgctgggca
     5461 ccggaggcgg gacgttcggc gccgcggccg ataccccgac cggtcaggcc cctgccggca
     5521 tcgcggtggc ggatgtcaat ggggacggcc gccccgacgc gatcctcacc aaccagaccg
     5581 acgccacggt gagcgtgctg ctcggcagca gcaacggcac gctgcggccc cgggtcgact
     5641 atccgaccaa cgtgttcccg ggcgtgccca tcgtggccga cctcaacggg gatggcaaac
     5701 ccgacctcgt gattccctac aacgtcggct tcaccaacaa caccgtgagc gtgctgctcg
     5761 gcaacggcga cggcacgttc cagcccaaga tcgactctct ctcgggccct gcaaccagcg
     5821 cggcgaccgt ggcggacctg aatcacgatg gcaagctcga cctcatcgcg tccagccagg
     5881 gcgagggcac ggtgagcgtg ctgctcggca acggtgacgg cacgttccag cccaaggtcg
     5941 accatttcct gcggttcacc gcggtggtca ccgcgatggc cgcggtggac ctcgaccgcg
     6001 acggcgcact cgatctcgtg atggccgagg acaacttcgg ccgggtcctc gtgctgttcg
     6061 gcaacggcga cggcacgttc cagcccgaga ccgactatcc ggcgggccag aaaccggtgt
     6121 cgctcgtgat cgcagacatc gatggcgacg gccaggtcga tctcgcggtc gccgacttca
     6181 cgggtaacac cgtcaacgtg ctgcggggca accgcgacgg aaccttccgg ccccagatcg
     6241 tctatcccgc agggaagact ccgatctcgc tcgcggtggc cgacgtcgat cacgacggca
     6301 ggcccgacct cgtcgtcgcc aatcgcgacc cggccaccgt gaccgtgctg cgcgcgcgct
     6361 gcctgcgctg acccgggcgg agcggcgctc gcggcgtgat cacgacgttg cagggtctac
     6421 ggccaccatc gcgccgtcgt ctcgatcgcc gcgatcacac cgagcacacc gtcctcgccg
     6481 ccgagccgct cgatgaccgg cgccatcact tccagctccg cgagcacgtc ccgccgctcc
     6541 cccagcacgc gctcgagctg gttccgccat cgggccgcaa tccggtcggg gccgagcgta
     6601 gcgaccagcg gcgcgaccgc ttcgagtccg cgggctcgct ccgtgcccgc atcggccgcg
     6661 cggtcccacg cttcgagcat gatgtccgcc tggcgtgatg catcgaccag cggtgcgagg
     6721 ctggcaagcg ccaaggccgt gtcgacattc gcggacacga cgaaagactt cgaggacttc
     6781 ttgatcgcac gcgcgagtgc gagcgccttc tcgaggagtc gtgagcggtg aggttcggtg
     6841 tgctggcgcg cgacctcggc gagcagctcg acgcgcatgt aattcgactc cccgtccgcg
     6901 aaggtctcgg cccattcgag gaacctgaac ctgatctcct ccgggaggcc cgtccgtccg
     6961 caccactgca cgcacgcacg gcaacgcgcg tcgacatacg tcatccggtc gaggatggag
     7021 atcgccgtcg ggagatcgcc gcaatctccc cggcgaacgg cgagcgccac cagggcgccg
     7081 tcgcgttcca tcagagcgtt gtggtcatca tcggagatca gcgccgacgc tgcatcgagc
     7141 cgctcgcacg ggattgccgg gatcagcgct tccaggagcc gatagcgatc gggaagccgc
     7201 agcacctccg ctgcctgcaa tcccagctcg caggcggcct cgcgctcgtc ctccgggagc
     7261 cggcgggcca gtggagcgat cgcggttgca ccgagctcgg cgtctgccgt gtcggccgcg
     7321 ccccggagga tcgtgcgcac cacctccgcg ggcacctcct cggggagctg gtcgagcgcg
     7381 agcctcaggt ggtgaccgtt gtcgagctcc ggcaggaaac ccagcgcccg atcccaccgt
     7441 cccaggcgtg cgttcgcggc gaacagcgcc gcggcagccc tggctcgctc caggccctgg
     7501 actgtcgaca gcgcgtccgc catccgatcg agctgcccgg ccgcggcgac cggcgcgagc
     7561 gcggccagcg agcgacctga cctccggccg ccggttccgt cggcgatggc ggcgagcatc
     7621 cggctcacga ccggttcacg cgccgcgggg ggcagcgtcc tcgtcagagc gccgatgcag
     7681 gtcacgcgtt gctcgacatc gccgatcagc cgcgcgtgcc gttcttcctc gatcaggac
//
LOCUS       DASVOR010000454         6752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_267001,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000454 DASVOR010000000
VERSION     DASVOR010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6752
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267001"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF516_08990"
     CDS             <1..250
                     /locus_tag="VF516_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107855.1"
                     /translation="LVPGLTVHTIESSCCGMAGGFGYEAEHYEISMRMAERSLLPAVR
                     RAPADALIVADGTSCRHQIADGAGRAAVHVARVLEAAL"
     gene            complement(302..1579)
                     /locus_tag="VF516_08995"
     CDS             complement(302..1579)
                     /locus_tag="VF516_08995"
                     /inference="COORDINATES: protein motif:HMM:NF025019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8107856.1"
                     /translation="MSPEDFRLNYAEAREVLNQRLNEPAPGRIQLLAGPRQVGKTTLL
                     LEIAARLGEAAVYAAADGPEAALPGFWERLWVRAEETAAAHGHAVVLLDEAHLLHNWA
                     GHLKGEWDRVRRRKIPIHVIATGSSALHLAAGSRESLAGRFERITLTHWSASSLAQVF
                     GVPPAEAADLLVRMGGYPGAFELRHDLARWAAYVRDAILEPAIGRDILALAAVRKPAL
                     LRQVFGVCVSSPAQIVSLQKIQGQLQDAGALETIAHYLALLEEAFLVAALGKYGNTPA
                     RRRAAPPKLITLNNALLAVSNPRGIPDRAIDPPRLGAWIENACLAHAWNAGQRVSYWR
                     EEPLEVDGVLEGSWGSWAIEVKTGPLAPRDLRGIAEFTRRHPSFRPLVLCDESAHATV
                     KRAGFAAMTWRDFLLTGRAGIQLHSNNYSSTPS"
     gene            2063..2242
                     /locus_tag="VF516_09000"
     CDS             2063..2242
                     /locus_tag="VF516_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107857.1"
                     /translation="MDEAGTLGCARYAQVALARYGQAIALAIGKMSGALFDALPPCEA
                     MIVGGGDYDANQCGD"
     gene            2217..3956
                     /locus_tag="VF516_09005"
     CDS             2217..3956
                     /locus_tag="VF516_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8107858.1"
                     /translation="MTRTNAGTDLIAPLVLLQLSDLHFGPYSRFSGCNLERLAAHCRQ
                     AIEEERRDLDWHETVDLVIVTGDVAEAAHPPEYVDAAVFFKALARELKLLPHRFVFVP
                     GNHDISWTKCRLVELQLEDGVFPPHELRARLDDVKFEHFETFVRDVYGGKARHEVKGA
                     AVTPLLRGAFIHDFADLGVSVAALNSCERESHRKEDHVGAMSDLQAQAVLDYWRKTPT
                     ELIRVLAVHHNPAAMATSAIEQWLEFLRSSAAHVPPDVVERIAASFVGFEGRDYLQRL
                     AADAHASLILHGHHHASEAHQAWAWRGRDAGGAGDARIISAGSWGLSLESGKLPKDQP
                     VVMQLIRLDPGAAEMKPVLLRYDPGARLSGDVRLGRFVPDPDTRTSRPIGLSLPPAHQ
                     QGPSHKRPRIDNNKTGTLLGQDVFGVVRNLDELRLAYETTVQSKHPCKGASAMDLDWT
                     LIDLYVEKSGERFGNDLSKEGLLDRLGLFSPLSPAGQSELHQAAVLCFCVRPERYSTG
                     SCCVRGWCNRGRIPAYRRLRSSELAGKATRREDQIRNQNHRYIGWGRAASGRAGSADG
                     RGPGGNLECGCSS"
     gene            4057..>6752
                     /locus_tag="VF516_09010"
     CDS             4057..>6752
                     /locus_tag="VF516_09010"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8107859.1"
                     /translation="MSEGQGSHPVDAAVALYVTNLLAFEGIGRGFEVIRQYIAKHGRE
                     CVTSAFVESPPAVVVRIARRSTNAQRYTDAATRDALREDRSSPAAPVPVYPDAEVEAL
                     SKRLEEARLRRDKLRAAEIATELVDREILELRRQLREGGQLRAGDALGDGRYLLVKVV
                     GRGGFAVVWEAYDYTARERVAIKVLHSNLAGDPLRRERFFRGAQAMMRLTHPAVVRVL
                     EPKAEEGGFYYFVMEFLPGGNLREAVLEQRLKKDDLLPSILQVAEALAMAHAAGMVHR
                     DVKPTNVVLDEHGRAKLTDFDLVGAHDTTGGTRTGALGTFVYAAPECLERPQDATPRA
                     DVYGLGMTAIFCLAGRDLSMDTFRNAERTIAKLECPRPVRQVLGRAVKWKPDDRFTNA
                     ATMLDALHDALDPATQVASIDDGMAGQIWSSSLSVTQSVEESGIDVGTVGLEISDRSP
                     WASTVALEDRPGRLFISHRTEDDAIVHALQQAMGRLFISHSTEDDAIVRALQQAMGDL
                     GLNAWIDSRQLRGGDPLWPDIQKAIESAEAYAVVVSPSGLQSSWVGKELRYALDVQKQ
                     RGMDAYPVIPLTLDDTPLGALGVIFDHEPIRIPLRSAPGGVEAALDAILVALRKREPA
                     DIALRPQPLAEPLEELVLELTDLKLEQVKEGARRATARARLVYEPATPGQRAVTSTQA
                     WRFVAPLGPLEADDLRWYLEKYAIWPGDVFRPRANKVEESLVAWGRLLHDAAMPTGPT
                     ASVLRAWGRADSRAGRRFSVLVDSTLEVGVPEVDAAAVREAATLLLGLPWELLHDGDS
                     FLFQGARPTRVRRRLPNMRDFDIAVVAPPIRILLVCARPEDAACSYIDHRVSALPLVD
                     AMEQLPGLVQLHILSPPTLPALREELDRARRARQLYHVLHFDG"
BASE COUNT         1158 a         2095 c         2237 g         1262 t
ORIGIN      
        1 gctcgtgccg gggctgaccg tccacacgat cgagtcgagc tgctgcggca tggcgggcgg
       61 gttcggatac gaggccgagc actacgagat ctcgatgcgg atggccgagc gcagcctgct
      121 cccggcggtg cgccgcgcgc cggccgacgc gctgatcgtc gccgacggca cgagctgccg
      181 ccaccagatc gccgacggcg ccggtcgggc cgcggtccac gtggcgcgcg tgctcgaagc
      241 ggcgctgtga cccgcgatcg cgggcacgtg gacctcgctc ggcctcgccg ccgcgatcga
      301 gctatgaagg tgtactcgaa taattattcg agtgcagttg aatgcctgct cggccggtca
      361 agaggaagtc gcgccacgtc atcgctgcga accctgcgcg cttcacggtc gcgtgcgctg
      421 actcgtcgca caggacgaga gggcggaagc tcggatggcg ccgggtgaac tcggcgatcc
      481 ctctgaggtc gcggggtgcg agggggccgg ttttcacctc gatcgcccac gatccccagc
      541 tcccttccag gacgccgtcg acctccagcg gttcctcccg ccagtaactc acgcgctgtc
      601 ctgcattcca cgcgtgcgcg aggcaggcgt tctcgatcca tgcccccagc cgcggaggat
      661 cgatggcgcg gtcggggatt ccccgaggat tggagactgc gagcagggcg ttgttgagcg
      721 tgatgagctt gggcggcgcc gcccgccggc gcgctggcgt gttgccatac tttcccaacg
      781 ccgccacgag gaacgcttcc tcaagcagcg ccaggtaatg ggcgatggtc tccagcgcgc
      841 ccgcatcctg cagctgcccc tggatcttct gcagcgacac gatctgggcc ggcgacgaca
      901 cgcacacccc gaatacctgg cgcagcagcg ccggcttgcg gaccgctgcc agcgcgagga
      961 tgtcgcgtcc gatcgccggc tccaggatgg catcgcggac gtaggccgcc cagcgagcca
     1021 ggtcgtggcg cagctcgaac gcgccgggat acccccccat cctgaccagc agatccgccg
     1081 cctccgcggg cggcacgccg aacacctgcg cgagcgacga tgcggaccag tgggtcagcg
     1141 tgatccgctc gaaccgaccc gcgaggcttt cccgcgagcc ggccgcgagg tgcagcgccg
     1201 acgatccggt cgcgatgacg tggatcggga tcttccggcg gcgcacgcga tcccactcgc
     1261 ccttgaggtg gcccgcccag ttgtggagca gatgcgcctc gtccagcagc acgaccgcgt
     1321 gcccgtgggc cgccgcggtc tcctcggcgc gtacccacag ccgctcccag aagccgggca
     1381 gggccgcttc cggaccatcc gcggcagcgt acaccgctgc ctcgcccagg cgagccgcaa
     1441 tctcaagcaa taatgtagtc ttaccaacct gacggggccc ggcgagcagc tggattcgcc
     1501 ccggtgccgg ctcattcagg cgctggttca ggacctcgcg ggcctcggca tagttgaggc
     1561 gaaagtcctc cggagacatg gtcgaataat tatttgacta tggcgaaatt gtcgagggtg
     1621 agccctcgtc gcgcacaaag cccccgatgc ggatgcgagt gcgggtgcgg gtgcgggtgc
     1681 gggatgggct ggtcggccgc gacgtcgaga ggagcggagc tgtgtttatc ccgcatcccg
     1741 aggtcgggcg tgccattcgg cgcgggtacg ccgcgcgcgg caactgctcg aagtcgacgc
     1801 agggctagga tcggacggca ctcgggggct catccggcct agctttcgga gccacggctg
     1861 agtgcgtgct gatccagtgg tcgccagccg cggaggcccg cgacggcttg cgcgcgaagc
     1921 tttgcgacga tgaccgggcc gaggtcgtgg tggaggctac ggcgttcatg aacgtccggg
     1981 tctcagccag ctgtcgtggc ttcaccaacc tggagctcgg gccgtgccgg ctgcggtcgc
     2041 tgcggctggc acgttggcgc ggatggacga ggccggcacg ctcggatgcg cacggtatgc
     2101 ccaggtcgcg ctcgcgcggt acggccaggc gatcgcatta gccatcggaa agatgagcgg
     2161 cgcgctgttc gatgcgctgc caccgtgcga ggctatgatc gttgggggag gcgattatga
     2221 cgcgaaccaa tgcggggact gacctgatcg caccgcttgt gctgctgcag ctctcggatc
     2281 tgcacttcgg tccgtatagc cggttttctg gctgcaacct cgagcggctg gcggcgcatt
     2341 gtcgtcaggc gatcgaagag gagcgcaggg atcttgattg gcacgagacc gtcgatctcg
     2401 tcatcgtgac gggggatgtt gcggaggcag cccacccgcc ggagtacgtt gacgccgcgg
     2461 tgttcttcaa ggcgctcgcc agggagctta agctattgcc gcaccgcttc gtgttcgtgc
     2521 ctggcaacca tgacatctcg tggacgaagt gccgtctggt cgagttgcaa ctcgaagacg
     2581 gcgtttttcc gccgcatgag ctgcgcgccc gcctcgacga tgtcaaattc gagcacttcg
     2641 agacgtttgt tcgcgacgtc tacggtggca aggctcgtca cgaggtcaag ggggccgcgg
     2701 tcacgcccct gctccggggc gccttcatcc acgactttgc cgaccttggt gtctcggtcg
     2761 cggcgcttaa ctcgtgcgag cgtgagtcgc accgtaagga ggatcacgtc ggcgcgatga
     2821 gcgacttgca ggcgcaagcg gtgctcgact actggcggaa gacaccgaca gagctcattc
     2881 gcgtgcttgc ggttcaccac aaccctgcag ctatggccac ctccgcaata gagcagtggc
     2941 tcgagttctt gcggtcatcg gcagcccatg tgccacccga tgttgtcgag cgcattgccg
     3001 ccagtttcgt cggcttcgaa ggtcgtgact atcttcagcg cctggccgcc gacgcacacg
     3061 catcgctgat cttgcacgga caccaccatg cctccgaggc acatcaggca tgggcatggc
     3121 gcggtcgcga tgcgggagga gccggtgatg ctcgcatcat ctcggccggt agctgggggc
     3181 tgtcactcga gtcaggtaag ctgccgaagg accagcctgt cgttatgcag ctcatccggc
     3241 tcgatccggg tgcggcggag atgaaaccag tgctgcttcg gtacgacccc ggtgcccgcc
     3301 tttcgggcga tgttcgcctt ggccgctttg ttccggaccc agatactagg acgagtcgcc
     3361 ccattgggct gtcgttgccg cctgcgcacc aacaaggccc ctcgcacaag aggccccgga
     3421 ttgacaataa taagactgga actctccttg gacaagatgt tttcggggta gtgcgaaatc
     3481 tggacgagtt gcgacttgca tatgagacaa ctgtccaatc aaagcatcca tgcaagggtg
     3541 cttctgccat ggatcttgat tggaccttga tcgatctcta cgttgagaaa tcaggagagc
     3601 gattcggaaa tgatctatcc aaggaaggtc tactcgaccg cctggggttg ttctctcctc
     3661 tttctccagc agggcaatcc gagctgcatc aggctgctgt tctctgcttc tgtgtgcgac
     3721 ctgaaagata ttccacaggc tcgtgctgtg ttcgtggttg gtgcaacaga gggagaattc
     3781 cagcgtaccg acgtctacgg tcctctgagc ttgcaggtaa agcaactcgt cgagaagatc
     3841 aaatccgaaa tcaaaaccat cgttacattg gatgggggag ggctgcgtca ggaagagccg
     3901 gatctgccga tggtcgtggt ccgggaggta atctcgaatg cggttgttca tcgtgattat
     3961 gaagcttcgg gtacaattcg tgtttggctg acgccggatg cattggaagt gcgaagtcca
     4021 ggggcctttc ctgaagggct ctcctgggaa agcttcatgt ccgaagggca aggatcgcat
     4081 cccgttgatg ctgcggtggc attatatgtc acaaatctac ttgcattcga gggtattggt
     4141 cgaggttttg aagtgattcg ccaatacatc gcgaaacacg gcagggagtg tgtcacttcc
     4201 gcatttgttg agagcccccc tgccgtcgtc gttcgcatcg ctcgacggtc aacgaatgca
     4261 cagaggtata ccgacgccgc aactcgagac gctctcagag aagacagatc gtctccggcc
     4321 gctcctgtcc ccgtttatcc cgacgccgaa gtcgaggcgc tgagcaagcg cctcgaagag
     4381 gccaggctgc gtcgcgacaa gctgcgcgcc gccgagatcg ctaccgaact tgtcgatcga
     4441 gagatccttg agctgcgcag acagttgcgc gaaggagggc aactccgcgc cggggatgcc
     4501 cttggcgacg gccgatatct ccttgtgaag gtcgtgggtc gaggaggctt cgcggtagtc
     4561 tgggaggcgt atgactacac agcgcgagag cgcgtcgcga tcaaggtatt gcactcgaat
     4621 ctcgccggcg atccgctccg ccgcgagcgc ttcttccgcg gcgcgcaggc aatgatgcgc
     4681 ctcacgcatc ctgctgtggt gcgcgtcctt gagcccaagg cagaggaggg aggtttctac
     4741 tacttcgtca tggagttcct gcctgggggc aacctgcgcg aggcagtgct cgagcagcgc
     4801 ctgaaaaagg atgatctctt gccatctatc ctccaggtcg ctgaggcgct tgccatggct
     4861 cacgcggccg gcatggtcca ccgtgatgtc aagccaacga acgtcgtcct ggatgagcat
     4921 ggccgtgcaa agctgaccga ttttgacctt gttggcgcgc atgacacgac cggcggcaca
     4981 agaaccggcg cgctcggcac ttttgtgtac gcagcaccgg agtgcctcga gaggccgcag
     5041 gatgcgactc cgcgcgcgga tgtgtatgga ttggggatga cagcgatctt ctgtctggct
     5101 ggacgagacc tctcaatgga tacgtttcgg aacgcggaga ggacgatcgc taagctcgaa
     5161 tgcccgcgtc cagttcggca ggtgcttgga cgagcagtta aatggaaacc ggatgatcgc
     5221 ttcaccaatg cagcgacgat gttggatgca cttcacgacg cgttggaccc tgcaacgcag
     5281 gtcgcgtcca tagatgatgg aatggcaggg caaatatggt catcttcgct ctctgtgact
     5341 cagtctgtcg aagaatcggg tatcgatgtc ggcaccgttg gccttgagat ttcggatcgc
     5401 tcaccgtggg ccagcacggt ggccctggag gacagaccgg gtcggctgtt catctcgcac
     5461 aggactgagg acgacgcgat cgtgcatgcc ctgcagcagg cgatgggtcg gctgttcatc
     5521 tcgcacagta ccgaggacga cgcgatagtg cgcgcccttc agcaggcgat gggcgatctc
     5581 ggcctgaacg cgtggatcga ctctcgccag ttacgcggcg gtgatccact atggccggac
     5641 attcaaaagg cgatcgaaag cgccgaggca tatgccgtcg tggtcagccc gagcggtctg
     5701 cagtcgagtt gggtcggcaa ggagcttcgc tatgcgctcg atgtgcagaa gcagcgcggt
     5761 atggatgcct atccggtcat cccgctgacc ctcgacgaca cacctctcgg tgcattggga
     5821 gttatcttcg accacgagcc gatccgcatc ccgctcagga gcgcccccgg cggcgtcgag
     5881 gccgcgctcg atgcgatcct cgtcgcgttg cgcaagcgcg agccggccga tatcgcgctg
     5941 aggccgcagc ccctagccga gccgctggag gagttggtac tggagctcac cgacctgaag
     6001 ctcgaacagg tcaaggaggg cgcgcgccgt gctactgcgc gggcgcgcct ggtgtacgaa
     6061 ccggcgacgc ccggacagcg cgcggtcaca agcacgcagg cctggcggtt cgtcgcgcca
     6121 ctcgggccgc ttgaggccga cgatctgcgc tggtacctgg agaagtacgc gatctggccg
     6181 ggcgacgtat tccgtccgcg ggcgaacaag gtcgaggaaa gcctcgttgc ctggggccga
     6241 ctcctgcatg acgcggcgat gcccacgggg ccgaccgcca gcgtgttgcg cgcctggggc
     6301 cgggcggaca gccgcgccgg ccggcggttc tcggtgcttg ttgactctac gctggaggtc
     6361 ggcgtaccgg aggtcgacgc ggcggccgtg cgcgaggccg ccacgctgct gctcggactg
     6421 ccgtgggagc tcttgcacga cggcgacagc tttctgttcc agggcgctcg gccgacccgc
     6481 gtgcgtcgac ggctgcctaa tatgcgtgac tttgacattg cggtcgtggc tcccccgatc
     6541 cgcatcctgt tggtctgtgc gcgccccgag gacgccgcat gcagttacat tgaccaccgc
     6601 gtgagtgcgc taccgctcgt cgatgcgatg gagcagctcc ctggcctagt ccagctccac
     6661 atcctctccc ctccgacgct gccagcgctg cgcgaggagc tcgaccgcgc gcgccgcgcc
     6721 cgccaacttt accacgttct ccactttgac gg
//
LOCUS       DASVOR010000455         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_325686,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000455 DASVOR010000000
VERSION     DASVOR010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325686"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..687)
                     /locus_tag="VF516_09015"
     CDS             complement(<1..687)
                     /locus_tag="VF516_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107860.1"
                     /translation="MFSIQRIYAFAVTPQRGATGEALVDPEGGPVTPTVALREVIANA
                     FSKIARGILTTVDFDLDENRTNVVRDDVIAIAFGATQTPRAAAFRLATRLAQTMDNRS
                     HAALLVIVVEHDDPKRRVSLLVFPREDVIQLGRQADEVLLNLLRNAFSTGSGLRKLAR
                     LEGHQSRTQFLSAEVLDFQLASAHKTTADFWIKTFLDAKPRIDSQTGSRQLAAALQRT
                     FDASSDDDRDA"
     gene            complement(870..1067)
                     /locus_tag="VF516_09020"
     CDS             complement(870..1067)
                     /locus_tag="VF516_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107861.1"
                     /translation="MERPQIERRAELVLGALRSAGRSGRSLFVRRGRGIVLTAFGRYF
                     AAQATAILNHVASVPHHKDHF"
     gene            complement(1242..1517)
                     /locus_tag="VF516_09025"
     CDS             complement(1242..1517)
                     /locus_tag="VF516_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107862.1"
                     /translation="MATVAGGAVRRASDDTDIRSSASGRGMKPLEKGVFLDVVLPRAR
                     EPREVRFNHLVREALELWVLHRRSRIRGNGSSALFLWDYDARLRSHE"
     gene            1663..2094
                     /locus_tag="VF516_09030"
     CDS             1663..2094
                     /locus_tag="VF516_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107863.1"
                     /translation="MLGALEGRYLVPPGIDVLELGLIDTAGRFELLPIDRRDKPLWDQ
                     VTHLLISDAEAERQREIVLSTYRALCARITEAEQLRSSVAEWSRSRVAHPVFGTIRFE
                     TIATWRSRRHGTEPLGERVLDRLRAEVARRVAARIGARLVR"
     gene            complement(2115..2417)
                     /locus_tag="VF516_09035"
     CDS             complement(2115..2417)
                     /locus_tag="VF516_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107864.1"
                     /translation="MATAKASKETALVLATGSCSSLADAFDLLRLDAIGVEALASAAN
                     TAIDEYRLPPSADRREFHRVHSLVSDTREKALALVELAENLKKTFIPRGEAADSDP"
     gene            complement(2308..>3149)
                     /locus_tag="VF516_09040"
     CDS             complement(2308..>3149)
                     /locus_tag="VF516_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107865.1"
                     /translation="PTAYALHRRLLGSDRPFVVCDPRRGDGPGSVRSLPNCRGGLDAL
                     LAAAGGSICIRARRLPADFDALTEALRGPGPTAQVFICLSDRDRVRDLLSPPLEIPTL
                     GGRAADLDRLAGECLEEATRALGVGRMRFSGVASDSVLRSVTSLTEIERVMLRLVALK
                     SSPNLSQAARRLRVAPVSLSRWLHRRRPTMVLRDVARLEYENAPEARRGTDATGAPLD
                     ESCQPPSERSGDHPHDNGEDHGKTPHGHGKGIEGDRTGPGDRIVQLAGGRLRPAAPRR
                     DRG"
BASE COUNT          536 a         1034 c         1065 g          514 t
ORIGIN      
        1 agcatcgcga tcgtcatcgc tagaagcgtc aaatgtacgt tgcagtgcag cggcgagctg
       61 gcgacttccg gtctgcgagt cgattcgtgg cttcgcgtcc aggaaggtct tgatccaaaa
      121 gtcggcggtc gtcttgtgtg ccgacgccaa ctgaaagtcg agcacctccg ctgacaagaa
      181 ctgtgttctc gactgatgcc cctcaagacg tgcgagtttt cgaagaccgg accctgtcga
      241 aaacgcattg cgcagaaggt tgagaagaac ctcgtccgct tgtcgtccga gctgaatgac
      301 atcctcgcga gggaaaacta gaagtgaaac ccgccgcttg ggatcgtcat gttccactac
      361 aatgactaaa agagctgcat gagagcggtt gtccattgtc tgtgcgagac gtgtggctag
      421 acgaaaagcc gcggcccgtg gagtctgggt cgcgccaaac gcgatggcta taacatcgtc
      481 gcgaactaca ttggtccgat tttcatcgag gtcgaaatcc actgtcgtga gaatgcctcg
      541 tgctatcttg gaaaaggcgt tcgcgatgac ctcacgcagc gctaccgtgg gggtgacggg
      601 accaccttcg ggatccacca atgcttcgcc tgttgcaccg cgctgaggcg ttaccgcgaa
      661 cgcatagatg cgttggatgg agaacacagg ttcccgtgta gactacaccc ggaccggggg
      721 atcgcgcgac ggcgctccac caagccggct tgtgcactgc accgacaggc cgagggggtg
      781 cgcgggggcc gcgaggggcc ttcgctaggt aaccgtgaaa tatttcggca gcattgaaag
      841 tagcgcccac agcgaccgta attgtgggct cagaagtgat ccttgtgatg gggcacgctc
      901 gcgacgtgat tcaggatcgc cgtcgcctgg gcggcgaagt agcgcccgaa cgcggtgagc
      961 acaatgccgc ggccgcggcg cacgaacagc gagcgcccgg atctgccggc tgagcggagc
     1021 gcgccaagga cgagttcggc gcggcgttcg atctgcgggc gttccacgac gagctgcttg
     1081 gtgcgggcgc actcccgctc gacgtcctcg agcggtccct cgtcgagccc acgccgacga
     1141 ggttatcgag agctggcctg cgacggtcag ctacgcggac tgatcctacc accgctctgg
     1201 tgacgttgac ctcggtcatc tgcgcggacc acattgcatg atcattcgtg cgacctcagc
     1261 cgcgcgtcgt agtcccaaag gaacagcgcg cttgagccgt tcccgcggat gcgcgatcgc
     1321 cgatgcagga cccagagctc gagggcttcg cggaccagat ggttgaagcg tacctcccgc
     1381 ggctcccggg cgcgcggcaa cacgacgtcc aagaagacgc ccttctcgag aggcttcatc
     1441 ccgcggccgg acgccgagct tcggatgtcc gtgtcgtccg atgctcgacg aacagcgccg
     1501 cctgcgaccg tggccatcct gaacgcgatc ggccacgctc gcacgcgtca gcgcaaggcg
     1561 aaggtgcccc gacccagact gctcggtcac gagatcctcc ggcgcgtcac gcgcgcggag
     1621 gtgccggcga actatcccct gaacccacgg tcggatcgcg cggtgctcgg cgcgctcgag
     1681 ggcaggtatc tcgtgccgcc gggcatcgac gtcctcgaac tcgggctcat cgacaccgct
     1741 gggcggttcg agctcctgcc catcgaccgc cgcgacaagc cgctgtggga ccaggtcacg
     1801 cacctgctca tcagcgacgc cgaggccgag cggcagcggg agatcgtcct ctccacgtat
     1861 cgcgcgctgt gcgcgcgcat caccgaagcc gaacagttgc ggtccagcgt cgcggaatgg
     1921 tctcgcagcc gcgtcgcgca tccggtcttc gggaccatcc gcttcgagac catcgcgacc
     1981 tggaggtcga gacgccatgg gaccgagccg ctcggcgagc gcgtgctcga ccgcctgcgc
     2041 gccgaggtgg cgcggcgggt cgcggcgagg atcggcgcgc gcctggtccg atgacacccc
     2101 tcctgctcgg cgagtcatgg gtcactgtcg gccgcttcac cgcgaggtat gaacgtcttc
     2161 ttgaggttct ccgccagttc gacgagcgcg agcgccttct ccctggtgtc gctcacgagc
     2221 gaatggaccc ggtggaactc gcgacggtcc gccgatggcg gcagccggta ttcgtcgatc
     2281 gccgtgttgg ccgcgctcgc gagcgcctca accccgatcg cgtcgaggcg cagcaggtcg
     2341 aaggcgtccg ccagcgagct gcacgatccg gtcgccagga ccagtgcggt ctccttcgat
     2401 gcctttgccg tggccatgcg gcgtcttgcc atgatcctct ccattgtcgt gcgggtgatc
     2461 gcctgaccgc tcgctcggcg gctggcacga ttcgtcaaga ggcgcgcctg tggcatccgt
     2521 gccgcgtcgc gcttcggggg cgttctcgta ctcgagccgc gcgacgtccc ggagcaccat
     2581 cgtcggccga cggcgatgca gccatcggct gagcgatacg ggcgcgacgc gcagccgacg
     2641 cgcagcctgg ctcaggttcg gcgacgactt gagcgccacg agacgcagca tgacgcgctc
     2701 gatctccgtg agcgacgtga cgcttcgcag gacggaatcg gaggcgacgc ccgagaagcg
     2761 catccggccg acgcccagcg cccgcgtcgc ctcttccagg cactcgccgg cgaggcggtc
     2821 gaggtccgcc gcacggccgc caagcgtggg gatctcgagc ggagggctga gcaggtccct
     2881 gactcgatcg cggtcggaca ggcagatgaa cacctgcgcc gtcggaccag ggcctcgcag
     2941 ggcttccgtc agcgcgtcga agtccgcggg caggcggcgg gcgcggatgc agatcgagcc
     3001 gccggcggcc gccagcaggg cgtcgaggcc gccccggcag ttgggcagcg acctcacgga
     3061 gccggggccg tcgccgcgac gaggatcgca gacgacgaag ggccggtcgg atcccaggag
     3121 ccgacggtgt aacgcgtacg ccgtcggga
//
LOCUS       DASVOR010000456         9993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208776,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000456 DASVOR010000000
VERSION     DASVOR010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9993
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208776"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1625)
                     /locus_tag="VF516_09045"
     CDS             complement(<1..1625)
                     /locus_tag="VF516_09045"
                     /inference="COORDINATES: protein motif:HMM:NF023643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N4-asparagine amidase"
                     /protein_id="HEX8107866.1"
                     /translation="MRSRLFRPRSFALATAGSALLVFGVAGGAPTVGSPNTTTADPPV
                     AVPDTTPCVVSLFSGLTFADFSPKRFSFAPPAGCAGPWAKVVLAADFSVTAGRQFDRT
                     ASIWLGGTNVYFGTTAEPSRTVAPAWHIESDLTDYSPLFRTAQPGMVQLDNLVNATFT
                     GILSGAAELRFYPVARHAEPPRTADVVLPLSNGPTGGTVGLATTASRLARTFTLPTNI
                     ERAVLDVYAQSQAGDEFWYTCVPDELTGPLQSCGATGFREAEITVDGRPAGVAPIYPW
                     IFTGGIDPLLWRPIPGVQTLSFTPYRVDLSPFAGVLSDGAPHEIAVSVFNADNFFATT
                     ASLLLFLDPHARTVRGAVTADTLTAAPTPAVTANITTAADGTISGPVDVTSTRQYRIA
                     GFVDTSHGRVSTEVTGEIRFANRQQFTITAATYHQQIAQTTTVVARTTRIGGGPPSEQ
                     LTALDWPLSVDFAFTSNTDGTFAQTTAIDQRFERSDTELAAGRLADVRIVSNHVAPAD
                     TLNFDATGALTGATGQVSAQQFFSADAHGHCFSR"
     gene            complement(1836..2669)
                     /locus_tag="VF516_09050"
     CDS             complement(1836..2669)
                     /locus_tag="VF516_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107867.1"
                     /translation="MTSKLAFSSSLLAAVASVVLAGATPAHAQPAPSDVPEDPYDDSE
                     DLAPPDESQPVTPDEAQAPAPAMQPPIEQPPPPPAGPPVVQQQVQPVQPVEAAPAAGG
                     QWVYTQQYGWVWQPYGNQYVYTPEDTSGSAYPYAYVYYPTYGWTWLAAPWVWGWGPHV
                     YFSLGRPGYYGWYHHPHYVGGYIGHGFVRGYGHGFGRGYGPVYRGGYVHGVRAPVYRG
                     GFRGGVGHIGGFHGGVGHVGGFHGGVGHVGGGFHGGVGHVSGGHFSGHVGGHIGGHGH
                     H"
     gene            complement(2964..3755)
                     /gene="radC"
                     /locus_tag="VF516_09055"
     CDS             complement(2964..3755)
                     /gene="radC"
                     /locus_tag="VF516_09055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEX8107868.1"
                     /translation="MIGGRAGGTSCSPAMERSLLVAGAMNLAISNQALEAGALGDLLG
                     DLTDRPRERLWRCGAHALGDAELISVVLGTGVRDCPVLELAGDLVRAVGGIAPLSRAS
                     PHELAQFNGIGPARGARLSAAFELGRRAVEVAHHRRTLGSAGDVYRCVAPRFAGVSQE
                     LLCVMGVDVRNGVLDVVEVARGSIAHVDVHPREVFRPLVRMAAAGGVLVHNHPSGDPT
                     PSRHDCVLTVRLREVGRLLGIPIIDHVVIGGRSYRSIAEWMGEAF"
     gene            4485..5408
                     /locus_tag="VF516_09060"
     CDS             4485..5408
                     /locus_tag="VF516_09060"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8107869.1"
                     /translation="MAQAWPSSQVSTTPIPAGQLRVIGQGHPLDHAIRRTMESFFQAD
                     FSGVRVYEGPAAQAMNALAFTLGEDIHFAPGLYDPASRGGVELLGHELTHVVQQRDGR
                     VVNPYGQGIAMVQNPELEAEADRMGQRIANELWAVPPPHPAALAQPKATGGALARPLP
                     RSQAVVAQRRGVLQAMDLLNVGVRFRTSKGSEYTVYRGDGIDRAKYYEPGQVTENPLK
                     NILYGDKETTEACLESRNRCAKYKLFPAKLGTWKIEFYDRWHNVIYSGVLRSWPELDL
                     YPVDMILKENKEGDMPDDRFHVGHRIVIIHQ"
     gene            5870..9466
                     /locus_tag="VF516_09065"
     CDS             5870..9466
                     /locus_tag="VF516_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8107870.1"
                     /translation="MNASRIACGYLLLLIACGPLAESTGGDVDRRYLIAMNSRRIDTR
                     VTPGAGVAAGDGRVLVKFPGPVTGEQLAALAATAQIYAYLPHDTFLVRPFAGLAASRP
                     TGASWTGAYLPEYKISPSVAALAQPAQLAAAETRTVMVTAFPDADLAGVAATAAGLPG
                     VTVVGSDRGARFSRVRLRVDDVALAGATDALAALAGVFWIDVEGRRELLNDTTVWVGQ
                     SGLDAGQTTPVFDHGIHGEGQVVGYIDTGIDADSCYFRDAARGLPPMNACDGGTTVDP
                     AQRKVIAVDFLAPSECAGGIAANEWDTQNHGSHVAGTIAGDNAASPILHDPGDGMAPG
                     ARLIVQDAGFLTDACGDLPGIGCPVVDLKPFFQQAYAQGARIHTNSWGDNENAFTQNN
                     YSAACQDVDEFMFSHPDFLILFAAGNSGPRAASVGSPSTAKNGLSVGATQRGTLASSM
                     ARFSSCGPAADGRFKPDLTMPGQNIISARNDRNVGSNNCNTITMSGTSMASPAAAGMA
                     ALVRQYYTDGFYPMGAPSAGHGFAPSAALIKATLLNSTQPMTGTGAGPLPDACQGWGR
                     VLLDNALYFDGQTRRLVATDDPGFPQGGAGQERSFTVHVTDGQPLRATLAWTDFPSTP
                     AAAVNLENDLDLVVTGPSGTFLGNVFAAGQSAAGGSPDRLNNIEQVLLAAPAPGFYTI
                     TVRAFNVPSSAQPFALVVSGAINRAPDTVTATAVTDTKISVSWTSVPGAFKYYVLQST
                     AGGPFNFVSSVLEPSTSLLVTGLVPNTTYSYQIIAVDTGGADSPPSAPVSATTLAVDP
                     NIPTHVTATAVSSFEIDVSWSTVASAAAYYVFESQAGGPFNFRTTVLAPGTTFKAAVL
                     AASTMYCYELASGLTDGEITAVSAPVCATTGAGVQPPTGLIVTATHDTRILVQWQPAS
                     SAVKYWVYQAAAGDPTFTLIGAVVGTTVFEAVNLMPTTEYCYRVTGVDVIGRESAPSS
                     TLCDTTLAPGLGGIQGYWKLDEGADTTAIDSSGFGRNGAITNATYSLQDRPNIDDDRS
                     ALSFSSSPDSAVFVPPATGLDLASPSFTVSFWTKIPAAGSVTFLGSSSGDCIHPAWEI
                     AQNASGLRIFDAGGPHSLGTSIPTGVWTHIAVVGSQTSPDLSAYVNGALVSTLPATQF
                     GLAHAPFYMGHVAGCPGGAVMLDSVQILSRAMSAAEVAAIGVPPPAPTHPTN"
     gene            complement(9473..>9993)
                     /locus_tag="VF516_09070"
     CDS             complement(9473..>9993)
                     /locus_tag="VF516_09070"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEX8107871.1"
                     /translation="FQVYDVNEQIGPDTVHFGANNWVLAGTTSIPKAWIGTLSGTDPG
                     FVDLANYDLRPQMSSPLVDKGTTLTVSTGPLGFPRALLMPTYHPPQHLLLAIGMGDGR
                     NNQGAPDLGAFEVNASAPGAPPQKPPPEMIGNQDMAGGCGCRTAGSQSGWLLWFPVAA
                     AVLTLARGRRRR"
BASE COUNT         1557 a         3543 c         3482 g         1411 t
ORIGIN      
        1 cggctgaagc agtggccgtg ggcgtcggcc gagaagaact gctgcgcgct gacctggccg
       61 gtcgcgccgg tgagcgcgcc ggtggcgtcg aagttcaggg tgtcggccgg cgcgacgtgg
      121 ttggacacga tgcggacatc ggcgagccgg ccggcggcca gctcggtgtc gctgcgctcg
      181 aaccgctgat cgatcgccgt ggtctgcgcg aacgtgccgt cggtgttgga cgtgaacgca
      241 aagtcgaccg acagcggcca gtccagcgcg gtcagctgct cgctcggcgg gccgccgccg
      301 atccgggtcg tgcgcgcgac gaccgtcgtg gtctgcgcga tctgctggtg gtaggtcgcc
      361 gcggtgatcg tgaactgctg gcggttggcg aaccggatct cgccggtgac ctcggtcgac
      421 acgcggccgt gcgaggtgtc gacgaagccg gcgatccggt actggcgggt cgaggtcacg
      481 tcgaccgggc cgctgatcgt gccgtcggcc gcggtggtga tgttcgccgt cacggccggg
      541 gtcggcgcgg cggtgagcgt gtcggcggtg accgcgccgc ggacggtccg cgcatgcggg
      601 tcgaggaaca gcagcagcga cgccgtggtc gcgaagaagt tgtcggcgtt gaacacgctg
      661 accgcgatct cgtgcggcgc gccgtcgctg agcacgccgg cgaatggcga caggtcgacg
      721 cggtacggcg tgaagctcag cgtctgcacg cccgggatcg gccgccacag cagggggtcg
      781 atgccgccgg tgaagatcca cgggtagatc ggcgcgacgc ccgcgggccg gccgtcgacc
      841 gtgatctcgg cctcgcggaa gccggtcgcg ccgcagctct gcagcgggcc ggtcagctcg
      901 tcggggacgc aggtgtacca gaactcatca ccggcctggc tctgcgcgta gacgtcgagc
      961 accgcgcgct cgatgttggt cggcagcgtg aacgtgcgcg cgagccggct cgcggtggtc
     1021 gcgagcccga ccgtgccgcc ggtcggcccg ttcgacagcg gcagcacgac gtcggcggtc
     1081 cgcggcggct cggcgtgccg tgcgaccgga tagaaccgca gctcggcggc gccggacagg
     1141 atgccggtga aggtcgcgtt gaccaggttg tcgagctgga ccatgcccgg ctgcgccgtc
     1201 cggaacagcg ggctgtagtc ggtcagatcg ctctcgatgt gccacgccgg tgcgaccgtg
     1261 cgcgacggct cggcggtcgt gccgaagtag acgttggtgc cgccgagcca gatgctcgcg
     1321 gtgcggtcga actggcggcc ggccgtcacc gagaagtccg ccgccagcac gaccttcgcc
     1381 cacgggccgg cgcagcccgc cggcggcgcg aagctgaacc gcttgggcga gaagtcggcg
     1441 aacgtcaggc cggagaacag cgacaccacg cacggcgtcg tgtccggcac cgcgaccggt
     1501 ggatcggcgg tcgtggtgtt cggcgagccg accgtcggcg cgccgccggc gacaccgaac
     1561 accaggagcg cgctgccggc ggtggcgagc gcaaacgagc gaggacgaaa cagccgagat
     1621 cgcacgaggc acctccgtgt ggcgcgcacg gtagtctcgg cggcgagcgc cggtcaatcc
     1681 gcgacacggg gtgcggcctc cacgcatcgg cgagtcgcga cgaggcatcg tgacccacgg
     1741 ggctcgcctg gcgcagaaag cttggtaagc gaggtccacg aaaacgaatg gactgcagcg
     1801 ccatgcgacg gcagcgccgc gcgatcggcg atcgatcagt gatggccgtg gccgccgatg
     1861 tgaccgccga cgtgccccga gaagtgcccg ccgctcacgt ggccgacgcc accgtggaag
     1921 ccgccgccga cgtggccgac gccaccgtgg aagccgccga cgtggccgac gccaccgtgg
     1981 aagccgccga tgtggccgac gccaccgcgg aagccgccgc ggtacaccgg cgcgcgaacc
     2041 ccatgcacgt agccgccgcg gtacaccggc ccgtagccgc gcccgaagcc gtgcccgtag
     2101 ccgcgcacga agccgtgccc gatgtagccg ccgacgtagt gcgggtggtg ataccagccg
     2161 tagtacccgg gccggccgag gctgaagtag acatgcgggc cccagcccca gacccacggc
     2221 gcggcgagcc aggtccagcc gtacgtcgga tagtagacgt acgcatacgg atacgccgac
     2281 cccgacgtgt cctcgggcgt gtacacgtac tggttgccat acggctgcca cacccagccg
     2341 tactgctggg tgtagaccca ttgcccgccg gccgccggag cggcctcgac cggctgcacc
     2401 ggctggacct gctgctgcac cacgggcgga cccgccggcg gcggaggcgg ctgctcgatg
     2461 ggcggctgca tcgccggggc aggagcttgc gcctcgtcgg gcgtcaccgg ctgcgactcg
     2521 tcgggcggag ccaggtcttc ggagtcgtcg tacggatcct cgggcacatc ggacggagcc
     2581 ggctgtgcat gtgcgggggt cgccccggcc agcaccaccg acgcgacggc cgcgaggagg
     2641 gaagatgaga atgcgagctt ggacgtcata cgggttctca acggtgaagg ttgtcggaca
     2701 gcccccaacc atgtcccttg catcggtggg gccagcctcc gtgacctgtc caaccccgcc
     2761 aattgcttcg agctacgccg cctgccagcg ctccggcgtg cccgggtgat aacggccgaa
     2821 gccagttcga gcgttgctcg atcaccacat gggcccgcgt ggtcggcacc ccggccgccc
     2881 gtccgcgcac cggcgtgcgc ttgcgcaccc ggccgatcgg gcggcacgcg gcagcgatca
     2941 cacgggctgc gagtctccgt cgatcagaac gcttcgccca tccattcggc gatcgagcga
     3001 tacgatcggc cgccgatcac cacgtgatcg atgatcggga tgccgagcag ccggccgacc
     3061 tcgcgcagcc gcacggtcag cacgcagtca tgccggctcg gcgtcggatc gcccgacggg
     3121 tgattgtgga cgagcacccc gcccgccgcg gccatgcgaa ccagcggccg gaacacctcg
     3181 cgcgggtgga catcgacgtg tgcgatgctg ccgcgcgcga cctcgacgac gtcgagcacg
     3241 ccattgcgga cgtcgacgcc catcacgcag agcagctcct gcgacacccc ggcgaaccgc
     3301 ggcgcgacgc agcggtacac gtcgcccgcg ctgcccagcg tgcggcgatg gtgagccacc
     3361 tccaccgcgc gccgcccgag ctcgaacgcc gccgacagcc gtgctccgcg cgccggcccg
     3421 atcccgttga actgcgcgag ctcgtgcggc gaggcccgcg acagcggcgc gatgccgccg
     3481 accgcacgca cgaggtcgcc cgccagctcc agcaccgggc agtcgcggac cccggtgccg
     3541 agcaccaccg agatcagctc ggcgtcaccg agcgcatgcg cgccgcatcg ccacagccgc
     3601 tcgcgcggcc gatcggtgag gtcgccgagc aggtcgccga gggcacccgc ttccagcgcc
     3661 tgattcgaga tcgcgagatt catggctccg gcgaccagca agctgcgttc catcgccggg
     3721 ctgcacgacg tcccgccagc gcgaccgccg atcacgccac cgcaactcct cgggaagaca
     3781 tcgactctgg agcgagcgcc ctcgttcgac gcggcgctcc tcgcggaccc tgcaggtgac
     3841 cgtctgtccg gtgttcgtcc ggatttcgga cgcgcgtccg gataccggcc aaaacccgga
     3901 caacagacaa cagacaacag acagcagacc tacgcagctc ggcagcgagg ggcgagtcct
     3961 tgccctggct catgccatac gggacgacga catccgcgcg acgtcgagct ccaggtcccc
     4021 gatcatgatc tcgagcggat gccgacctcg tgccgccaaa aaagttgttg atctgcacgg
     4081 ccgagcccaa gatcgacgcc ggcactgcca ggcagcagcg acacgctcgg cccgtggcgc
     4141 gtcatcccgt cacgttcagc cgcggcgatg gtcgctgcgc tgtccagcgt ccatggggga
     4201 ggccaacgat gaagaccggc agcgcgaagg ctggctctgt attgcagggg tacttccgta
     4261 ccgaccgcct tcgaggggcg gcgcacaggg ccgcggtcgc ccagtcgaag ggcccggcgc
     4321 gatcggctcc gcagccacca cgacccgagc tcctgccggt gaggatgcgc ggagtggcag
     4381 cccagccccg ggccgagccc gggtcgggtc gatcgtggcg cggagcgccg cggccggacc
     4441 tgctgccggg catgggcccg acgagagccg gggagcggtt gcgcgtggcg caggcgtggc
     4501 cgagcagcca ggtctcgacc acaccgatcc cggccgggca gctgcgggtg atcggtcagg
     4561 ggcacccgct ggaccatgcg atccgtcgaa ccatggagag cttctttcag gcagacttct
     4621 ccggggtgag ggtgtacgaa gggccggccg cccaggcgat gaatgcgctg gcgttcacgc
     4681 tgggcgagga catccacttc gcgccggggc tgtacgatcc ggcgagccgc ggcggggtgg
     4741 agctgctcgg acacgagctg acacacgtgg tgcagcagcg ggatggacgc gtggtcaatc
     4801 cgtatgggca ggggatcgcg atggtgcaga atccagagct ggaggcggag gcggatcgga
     4861 tggggcagag gatcgcgaac gagctgtggg cggttccgcc acctcatcct gcagcgctgg
     4921 cgcagccaaa ggcgaccggc ggggcgctgg cgcgaccgct gccgcgctcg caggccgtcg
     4981 tggcacagcg ccgaggagtc ctgcaggcca tggacctgtt gaacgtggga gtgcggttca
     5041 ggacctcgaa gggcagcgag tacaccgtat atcgaggaga cggcatcgac cgggcgaagt
     5101 actacgagcc cggtcaggtc accgagaacc cattgaagaa tatcttatac ggagataagg
     5161 agaccacgga ggcgtgcctc gagtcccgga accggtgcgc caagtacaag ctgttccccg
     5221 ccaagctcgg aacgtggaag atcgagtttt atgaccgctg gcacaacgtg atctactctg
     5281 gagtcctgcg aagctggccc gagctggacc tctatcccgt cgatatgatc ctcaaggaaa
     5341 acaaggaggg agacatgccc gacgaccgat ttcatgtagg tcacagaatc gtcatcatcc
     5401 atcagtagac cgtgacgtcg agccgcctga tcgccgggct acctcccagc tgctcgggat
     5461 gatcaggatc gatcagtccg tccacaggat ggaagacgtc tggtcgttca tcacgcccat
     5521 ggagctgcag gcgctgccgc agatcccgcg cgtggctcgt cggtctcgcc cgggaagaag
     5581 tcgccttcga tcgcgatacc ggaagcgcgg cgatcccgct caccacaggc gccgacatcg
     5641 ccgtaccggc cccgtcgcag tggcgcgcga tgttgcgggc agtgaccacg tcgagaccac
     5701 gcggtgttgc ccatcgaccg gccagccggc cggcgtgcgg agaagcatgc gaacctcttg
     5761 cacgcacgca catgtgactt ccgggcgccg tcgggtcatc gatttgctag cgctgctcac
     5821 ttgttcgcgg tcgttcgccc gtggtgctgt gcgcgcacga ggacgaacga tgaatgcttc
     5881 ccggattgcg tgtggttacc tgctgctcct gatcgcgtgc ggcccgctcg cagagtccac
     5941 gggcggcgat gtcgatcgcc ggtacctgat cgcgatgaac tcccggcgga tcgacacccg
     6001 ggtgacgccc ggcgccgggg tagcggcggg cgatggccgc gtgctcgtca agttccccgg
     6061 cccggtcacc ggcgagcagc tcgcggcgct cgcggcgacc gcccagatct acgcgtactt
     6121 gccgcacgac acgttcctcg tccgcccgtt cgccgggctg gccgcgtcgc ggccgaccgg
     6181 cgcgtcgtgg accggcgcgt acctgcccga gtacaagatc tcgccctccg ttgccgcgct
     6241 ggcgcagccg gcgcagctcg cggcggccga gacccgcacc gtgatggtca cggcgttccc
     6301 cgacgcggat ctggcgggcg tcgccgcgac ggcggccggc ctgcccggcg tgaccgtggt
     6361 cggctcggat cgcggcgcgc ggttttcgcg cgtccggctg cgcgtcgacg acgtggcgct
     6421 cgccggcgcg accgacgcgc tcgccgcgct cgccggcgtg ttctggatcg acgtcgaggg
     6481 ccggcgcgag ctgctcaacg acaccacggt ctgggtcggc cagtccgggc tcgacgcggg
     6541 acagaccacg ccggtgttcg atcacggcat ccacggcgag ggccaggtcg tcgggtacat
     6601 cgacaccggc atcgacgccg acagctgcta cttccgcgac gccgcgcgcg gtctgccacc
     6661 gatgaacgcg tgcgacggcg gcaccaccgt cgatcctgca cagcgcaagg tgatcgcggt
     6721 cgacttcctg gccccgagcg agtgcgcggg cgggatcgcg gcgaacgagt gggataccca
     6781 gaaccacggc tcgcacgtcg cgggcacgat cgccggcgac aacgccgcca gtccgatcct
     6841 ccacgacccc ggcgacggca tggcacccgg cgccaggctg atcgtgcaag acgccgggtt
     6901 cctcaccgac gcctgcggcg acttgccggg catcggctgc ccggtcgtcg atctcaagcc
     6961 gttcttccag caggcgtacg cacagggcgc gcgcatccac accaactcgt ggggcgacaa
     7021 cgagaacgcg ttcacccaga acaactactc ggccgcgtgc caggacgtcg atgagttcat
     7081 gttcagccac cccgacttcc tgatcctgtt cgccgccggc aactcgggcc cccgcgcggc
     7141 gtcggtcggc agcccgtcga ccgcgaagaa cggcctctcg gtcggcgcga cccagcgcgg
     7201 caccctggcc agctcgatgg cccggttctc gagctgcggc ccggccgccg acgggcggtt
     7261 caagccagat ctcaccatgc cgggccagaa catcatctcg gcgcgcaacg atcgcaacgt
     7321 cggctcgaac aactgcaaca cgatcacgat gagcggcacc agcatggcca gccccgccgc
     7381 cgcgggcatg gcggcgctgg tccgccagta ctacacggac ggcttctatc cgatgggcgc
     7441 gcccagcgcc ggccacggct tcgcgccgag cgccgcgctg atcaaggcca cgctgctcaa
     7501 ctcgacccag ccgatgaccg gcaccggcgc cggcccgctg cccgacgcct gccagggttg
     7561 gggccgcgtg ctgctcgaca acgcgctgta cttcgacggc cagacgcggc ggctggtcgc
     7621 caccgacgat cccggcttcc cgcagggcgg cgccggccag gagcgcagct tcaccgtcca
     7681 cgtcaccgac ggccagccgc tgcgcgcgac gctcgcgtgg accgacttcc catcgacgcc
     7741 cgccgcggcc gtgaacctcg agaacgatct cgatctcgtg gtcaccggtc catcgggcac
     7801 gttcctgggc aacgtcttcg ccgccggtca gtcggccgcc ggtggcagtc cggatcgcct
     7861 caacaacatc gagcaggtgc tgctcgccgc gccggcgccg ggtttttaca cgatcaccgt
     7921 gcgcgcgttc aacgtgccga gctccgcgca gccgttcgcg ctggtggtca gcggcgccat
     7981 caaccgcgcg ccggatacgg tgactgcgac cgcggtcacc gacaccaaga tctcggtgtc
     8041 ctggacctcg gtgcccgggg cgttcaagta ctacgtgctc cagtccaccg ccggtggtcc
     8101 gttcaacttc gtcagctcgg tgctcgagcc gtcgacctcg ctgctggtga ccgggctcgt
     8161 gccgaacacg acgtactcct atcagatcat cgcggtcgac accggcggag ccgactcgcc
     8221 gccgtcggct ccggtgtcgg ccacgacgct ggcggtcgat ccgaacatcc cgacccacgt
     8281 caccgccacg gccgtgtcga gcttcgagat cgacgtgagc tggtcgacgg tggccagcgc
     8341 ggccgcgtac tacgtgttcg agtcccaggc cggcggcccg ttcaacttcc gcacgaccgt
     8401 gcttgccccc gggacgacgt tcaaggcggc cgtcctcgcg gccagcacga tgtactgcta
     8461 cgagctcgcg agcggcctga ccgacggcga gatcaccgcg gtgtcggcgc cggtgtgcgc
     8521 gaccaccggg gccggcgtgc agccgcccac gggtctgatc gtcaccgcga cgcacgatac
     8581 ccggatcttg gtccagtggc agccggcctc cagcgccgtg aagtactggg tctaccaggc
     8641 cgcggccggc gacccgacgt tcacgctgat cggcgcagtg gttggcacga cggtgttcga
     8701 ggccgtcaac ctgatgccga ccaccgagta ctgctaccgc gttaccggcg tggacgtcat
     8761 cggcagggag tcggctccat cgtccaccct ctgcgatacg accctcgcgc cgggcctcgg
     8821 cggcatccag ggctactgga agctcgacga gggcgccgac accaccgcga tcgactcgtc
     8881 gggcttcggc cgcaacggcg cgatcaccaa cgccacctac tcgctgcagg accggccgaa
     8941 catcgacgat gaccgctcgg cgctgtcgtt ctcgtccagc ccggactcgg cggtcttcgt
     9001 tccgccggcg accggcctcg acctggcgag ccccagcttc accgtgtcgt tctggaccaa
     9061 gatccccgcc gccggaagcg tcacgttcct cggcagcagc tccggcgact gcatccaccc
     9121 cgcctgggag atcgcacaga acgccagcgg cctgcgcatc ttcgacgccg gcggaccgca
     9181 ctccctcggc acctcgatcc cgaccggcgt gtggacgcac atcgcggtcg tcggatccca
     9241 gacctctccc gacctcagcg cctacgtcaa cggcgccctg gtcagcacgc tgccggcgac
     9301 ccagtttggc ctggcacacg ccccgttcta catgggccac gtcgccgggt gccccggcgg
     9361 cgcggtgatg ctggacagcg tgcagatcct gagccgcgcg atgtccgccg cggaggtcgc
     9421 cgcgatcggc gtgccgccgc ccgcaccgac ccacccgacc aactgacggc gatcagcgcc
     9481 gccgccggcc gcgagcaagc gtcaggacgg ccgcagcgac cgggaaccag agcagccagc
     9541 cggactgcga gccggcggta cggcagccgc agccaccggc catgtcctgg ttgccgatca
     9601 tctccggggg aggcttctgt ggcggagcgc cgggcgcgct ggcgttcact tcgaacgcac
     9661 cgaggtccgg cgcgccctgg ttgttgcggc catcgcccat cccgatggcc agcaggagat
     9721 gctgtggcgg atggtacgtc ggcatgagca gcgcccgcgg gaaacccaag ggtccggtgg
     9781 agaccgtcag ggtcgttccc ttgtcgacga gcggcgacga catctggggc cgaagatcgt
     9841 agttcgcgag atcgacgaag cctgggtcgg tgccactcaa cgtgccgatc catgccttgg
     9901 ggatgctggt cgtgccggcg aggacccagt tgttcgcccc gaagtgaacc gtgtccgggc
     9961 cgatctgctc gttgacgtcg tagacctgga agc
//
LOCUS       DASVOR010000457         3669 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_471933,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000457 DASVOR010000000
VERSION     DASVOR010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471933"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1702)
                     /locus_tag="VF516_09075"
     CDS             complement(200..1702)
                     /locus_tag="VF516_09075"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8107872.1"
                     /translation="MRGSLGQILVQQGVIDASALSDALAHQSGRHPLASELYMLGYAS
                     ERQLCLALSAQTGWPGIVLDESVIRLDVLEYVSLDWARIFAALVVYEDAQTLVVAAAR
                     PEDAVAPARELATSRGKNVELRIALDITLARTIRVAFSRWKTGELFLAGPEADRSGPY
                     LAVVHPDRDDDEHRRAQRALAEDAARNIERDDELPSEESAGGATSTGDWQTSLSNTAI
                     GVEEIEREERTMEIAAELDRGNEEPDTHRGRMRALVVDPDPPNRIQMVSQLERLDYEC
                     QAAATGMQALSLLGSLYFDIVFADVATPELDGLRLCRAIKRSKRLGKCRVIVTTAVVD
                     SGQIADEDLQRHGADGYLEKPLDSRRLRRLLRDLSSEERGDHEALLADALGRYHAGDI
                     EGAINRLRGALVGDPSSPKLHFMLANMLQRASRWAEAIDEYEAVVELQPAYFPALTRL
                     AYLYFRQGLHARAVETWRRALPVCDDPALRRNIELFMRKLVADMAKTESA"
     gene            2045..2533
                     /locus_tag="VF516_09080"
     CDS             2045..2533
                     /locus_tag="VF516_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX8107873.1"
                     /translation="MTHDFRIGEKAVYPAHGVAEVIGIEKKEINTSVCAFYVLKVLAT
                     GMQILVPKDKADQVGLRHVATASEIDEVFDILREKDVHIDKQTWNRRYRGFMEKIKTG
                     SLFDVAEVYRDLYRLKSSKVLSFGERKMLDNAKQLIVKELAVARKWSEEKVEAELEKA
                     CA"
     gene            2609..3118
                     /locus_tag="VF516_09085"
     CDS             2609..3118
                     /locus_tag="VF516_09085"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEX8107874.1"
                     /translation="MQVHVARAELWTLPVDAVVNPANSLGIMGGGIGGSLRRQGGDVI
                     QQAAMQVAPIAVGAAIVTTAGQLPAKNVIHVPTMEEPGMKIGAENVRRAARAALIAAD
                     RNHFKVIAMPGIGTDLGEVPMDEAARAIVEEIRAHRRPNPETIYLVDTSDDMVFALEE
                     ALRNAQANL"
     gene            3268..3645
                     /locus_tag="VF516_09090"
     CDS             3268..3645
                     /locus_tag="VF516_09090"
                     /inference="COORDINATES: protein motif:HMM:NF012717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEX8107875.1"
                     /translation="MPAGWAIVLAVGFGGLFTALAVLIGPKAVNPEKLKPFECGSEPI
                     GTPRGRYSVRFYQVAILFLVFDIEAAFMYPWALLYNQLSHATAAPGIFLVSVFGFVEM
                     LVFVAILVVALAYVWRKKAIGWD"
BASE COUNT          593 a         1211 c         1281 g          584 t
ORIGIN      
        1 gttcttcacg ctgtcgcccg acgacggcga cgcgatcgcc gccgccgagc gcgcggccga
       61 ggccgccggc ggctggctgc tcggcgcgcg ggcgcaccgg ctcgacagct acctgcgcga
      121 gctgcgcgac gcgctcgacc ccgagcggat catgaacccc ggcgcgctgg cctgagccga
      181 ggcaccctgc gccgcgcggc tacgccgact cggtcttggc catgtcggcg accagcttgc
      241 gcatgaacag ctcgatgttg cggcgcagcg ccgggtcgtc gcataccggc agcgcgcgcc
      301 gccaggtctc gaccgcgcgg gcgtgcaggc cctggcggaa gtacaggtag gcgagccggg
      361 tcagcgccgg gaagtacgcg ggctggagct cgaccacggc ctcgtactcg tcgatcgcct
      421 cggcccagcg cgacgcgcgc tgcagcatgt tggcgagcat gaagtgcagc ttggggctcg
      481 acggatcgcc gaccagcgcg ccgcgcagcc ggttgatcgc gccctcgatg tcgccggcgt
      541 ggtagcggcc gagcgcgtcg gcgagcagcg cctcgtgatc gccgcgctcc tcgctcgaca
      601 ggtcgcgcag caggcggcgc agccggcgcg agtcgagcgg cttctcgagg taaccgtcgg
      661 cgccgtggcg ctggaggtcc tcgtcggcga tctgccccga gtcgaccacc gcggtcgtga
      721 cgatcacgcg gcacttgccg agccgcttgc tgcgcttgat cgcgcggcac agccggaggc
      781 cgtcgagctc gggggtcgcg acgtcggcga acacgatgtc gaagtacagg ctgccgagca
      841 gcgacagcgc ctgcatcccg gtcgccgcgg cctggcactc gtagtcgagc cgctcgagct
      901 gcgacaccat ctggatccgg ttgggcggat ccggatcgac gaccagcgcg cgcatccggc
      961 cgcggtgggt gtcgggctcc tcgttgccgc ggtcgagctc cgcggcgatc tccatggtgc
     1021 gctcctcgcg ctcgatctcc tcgacgccga tcgccgtgtt cgacagcgag gtctgccagt
     1081 cgccggtcga ggtcgcgccg cccgccgact cctcggacgg cagctcgtcg tcgcgctcga
     1141 tgttgcgcgc ggcgtcctcg gcgagcgcgc gctgggcgcg gcggtgctcg tcgtcgtcgc
     1201 ggtcggggtg gaccaccgcg aggtaggggc ccgagcgatc ggcctccggt ccggcgagga
     1261 acagctcgcc ggtcttccac cgcgagaacg cgacgcggat cgtgcgggcg agcgtgatgt
     1321 cgagcgcgat ccgcagctcg acgttcttgc cgcgcgatgt ggcgagctcg cgcgccgggg
     1381 cgaccgcgtc ctcggggcgc gccgcggcga ccaccagcgt ctgggcgtcc tcgtagacca
     1441 ccagcgcggc gaagatccgc gcccagtcga gcgagacgta ctccagcacg tccagccgga
     1501 tcaccgactc gtcgagcacg attcccggcc agccggtctg cgccgacagc gcgaggcaca
     1561 gctgacgctc gctcgcgtaa cccagcatgt agagctcgga ggcgagcgga tgtcgtccgc
     1621 tctggtgcgc gagcgcatcc gacaacgcgc tcgcgtcgat cactccttgc tggacgagga
     1681 tctgaccgag cgagccgcgc acgttctctg cagggtatca ggacctcgcc acgggtcaca
     1741 ttttcttcac catgcaagta gttgagatct cgactgaccg tgcctggccg atccgctcgt
     1801 ggaagaatag tctggccatt gccatcgaca ttgatatcct ccgctgccct cagagggggg
     1861 ccgatcgccc cttcgctgac gggaaatgtc ggacgtgaca ggctgtcgca cggcagcgaa
     1921 aggaagccgg ctcccacgtt tcgagctaac taatcaatag aaaaggcttt tcgggaacca
     1981 cgcccaaggt ggcacccgcg ttgcttcgcg ggtaggtccc cgaaccacgc agaggagaga
     2041 caccgtgact cacgacttca gaatcggcga aaaggccgtg tacccagcgc atggcgtggc
     2101 cgaggtgatc gggatcgaga agaaggagat caacacctcc gtctgcgcct tctatgtact
     2161 gaaggtgctc gcgaccggga tgcagatcct ggtgccgaag gacaaagccg atcaggtcgg
     2221 gctgaggcac gtggccactg cgtcggagat cgacgaagtg tttgatatcc tgcgtgagaa
     2281 ggacgttcac atcgacaagc agacgtggaa ccggcgctac cgcgggttca tggagaagat
     2341 caagacgggc tcgctgttcg acgtcgccga ggtctatcga gatctctacc ggctcaagag
     2401 cagcaaggtg ctgtcgttcg gggagcgcaa gatgctggac aacgccaagc agctcatcgt
     2461 caaggagctg gccgtggcgc ggaaatggag cgaggagaag gtcgaggccg agctggagaa
     2521 ggcctgcgcc tgacggggat cctcggcgga tcccgggcct ccaggtcacg gttgtgttgc
     2581 gagcggttga tgtgctaggg catcgcgcgt gcaagttcac gtagcgcgcg cagagctctg
     2641 gacgctgccg gtcgatgctg ttgtcaaccc ggcgaattcg ttggggatca tgggtggcgg
     2701 tatcggtggc tcgctgcgtc gccagggcgg cgacgtgatc cagcaagcgg cgatgcaggt
     2761 cgcgccgatc gcggtcgggg cggcgatcgt gacgaccgcg gggcagctgc ctgccaagaa
     2821 cgtcatccat gtcccgacca tggaagagcc cgggatgaag atcggggccg agaacgtccg
     2881 gcgcgccgcc cgcgccgcgc tgatcgccgc cgaccgcaac cacttcaagg tcatcgcgat
     2941 gcccgggatc ggcactgatc tcggtgaggt cccgatggac gaggccgccc gcgcgatcgt
     3001 cgaggagatc cgcgcgcacc gccggcccaa ccccgagacg atctacctgg tcgacaccag
     3061 cgacgacatg gtgttcgcgc tggaagaggc gctccgcaac gcgcaggcga acctgtaact
     3121 cggcgcccgg catcctaccc gtcgcggcgt cccaggccgc ctcgcatcag agttaagcgc
     3181 gcgatcgctc actgaaaacc ggcgcgcgtg cgttgaccgc ttgcgcgcga gccatatact
     3241 cgcctcgctt tgcagctcca agcatacatg ccagccgggt gggcgatcgt gctcgccgtc
     3301 ggattcggcg gtctgttcac agcgctcgcg gtgctgatcg gccccaaggc cgtcaacccg
     3361 gagaagctca agccgttcga gtgcggcagc gagccgatcg ggacgccgcg cgggcggtac
     3421 tcggtgcggt tctaccaggt cgccatcctg ttcctggtgt tcgacatcga agctgcgttc
     3481 atgtatccgt gggcgctgct ctacaaccag ctgtcgcatg ccacggcagc gcccggaatc
     3541 ttcctggtga gcgtgttcgg gttcgtcgag atgctggtgt tcgtcgcgat cctcgtcgtc
     3601 gcgctcgcct acgtctggag aaagaaggcg atcggatggg actagagctg accaccacgc
     3661 gcctggaag
//
LOCUS       DASVOR010000458         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_530275,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000458 DASVOR010000000
VERSION     DASVOR010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_530275"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF516_09095"
     CDS             complement(<1..183)
                     /locus_tag="VF516_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107876.1"
                     /translation="MWSRAALAGALIASVSCNRSEGGDSAGSALVAGAGSAKAVGPGS
                     FSTDLARELAGPDKGSG"
     gene            complement(315..412)
                     /gene="ffs"
                     /locus_tag="VF516_09100"
     ncRNA           complement(315..412)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VF516_09100"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(492..576)
                     /locus_tag="VF516_09105"
     tRNA            complement(492..576)
                     /locus_tag="VF516_09105"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(540..542),aa:Ser,seq:gga)
     gene            complement(712..794)
                     /locus_tag="VF516_09110"
     tRNA            complement(712..794)
                     /locus_tag="VF516_09110"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(758..760),aa:Ser,seq:cga)
     gene            complement(859..1320)
                     /gene="tadA"
                     /locus_tag="VF516_09115"
     CDS             complement(859..1320)
                     /gene="tadA"
                     /locus_tag="VF516_09115"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEX8107877.1"
                     /translation="MTDEHWMGLALDEARAAAASGDVPVGAVIVVGDRIVGRGRNRRE
                     VDRDPTAHAEIVALREAATVLGQWRVEGTLVVTQEPCPMCAGALVNARVARLVFGCPN
                     PKAGAVATLYQIVSDPRLNHRVAVTAGVRAAECAAVLQAFFAELRRGPSYS"
     gene            complement(1386..1458)
                     /locus_tag="VF516_09120"
     tRNA            complement(1386..1458)
                     /locus_tag="VF516_09120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1423..1425),aa:Arg,seq:acg)
     gene            complement(1527..1620)
                     /locus_tag="VF516_09125"
     tRNA            complement(1527..1620)
                     /locus_tag="VF516_09125"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1584..1586),aa:Ser,seq:gct)
     gene            complement(1798..1880)
                     /locus_tag="VF516_09130"
     tRNA            complement(1798..1880)
                     /locus_tag="VF516_09130"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1844..1846),aa:Ser,seq:tga)
     gene            1987..2196
                     /locus_tag="VF516_09135"
     CDS             1987..2196
                     /locus_tag="VF516_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107878.1"
                     /translation="MTVVVEVDVAVDVEPIMDLDVALRCRCKVNDGVNDGVDANVAVD
                     VEALVKVIVEDKVEEVRIIFRSRLL"
     gene            complement(2218..3486)
                     /gene="serS"
                     /locus_tag="VF516_09140"
     CDS             complement(2218..3486)
                     /gene="serS"
                     /locus_tag="VF516_09140"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEX8107879.1"
                     /translation="MDDVLRRGPEVAPLVAGWRSADERRRKLQGELDSLRQQRNAANE
                     KMAKLDKKSAEFAAARDDMKTLAGRIKEGEGELAQLEAVCEQHLLTIPNAPHASVPDG
                     AGEADNPVVRTWGDKPAFSFTPRPHDELGRALGVFDFEGGARIAGARFTVLRGAASRL
                     NRALLNYMMDLHTQHGYQEAWPPAIVRRAALIGTGQLPKFAEDVFRLDIPVGPDHDAD
                     NDLFLSSTAEIQMTNLYMDQILDGETLPRRHCAYAACFRSEAGAAGRDTRGLIRQHQF
                     DKVELVKITTPDTSYDELEAMVRDAERVLQGLGLHYRVITLCTGDLGFSAAKTYDIEV
                     WLPGQDAYREISSCSNCTDFQARRAKIRYRPAPGDKPRPVHTLNGSGLPLGRTLVAIF
                     EQYQQADGSILVPAALRPYTGGLERVSTGR"
     gene            3604..>4179
                     /locus_tag="VF516_09145"
     CDS             3604..>4179
                     /locus_tag="VF516_09145"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8107880.1"
                     /translation="MESLAAGLPLLERSAAVTADDPDQALAERFRDGDRTAFDALVRR
                     HQKGMWRLIRRYVKSDADAADVTQLAFVRAFRGLATFRGTATVRSWLYRIAINCALSW
                     LRDHRREQPAEIGEDALTDDNPAPAQLSASDDRARLRAAIAQLPPKQKLVLELRVFDD
                     LAFREVADLADCSENTAKVNFHYAVKRLRDIL"
BASE COUNT          684 a         1480 c         1359 g          656 t
ORIGIN      
        1 ccccgagccc ttgtccgggc ccgcgagctc cctggccaga tccgtgctga agctgcccgg
       61 gcccaccgcc ttggcgctgc cggcgcccgc gaccagcgca ctcccggcgc tgtccccgcc
      121 ttcggaccgg ttgcaagaaa ccgaagcaat cagggcgcct gcaagcgcgg ctctggacca
      181 catccggatg tctatagcac ccccgaggac ccgcctgagg ctccgaaaaa ggctccgaaa
      241 aaggctccga aaaaggctcc gaaaaaggct ccgaaaaagg ccgtgagcga cgactcgtcg
      301 cgaatcgtcg ctcacaggga aaggtggccc caggcacccg ctatttccgc taccgttgct
      361 tccttccgga cctggcgggg ttcacagctg ttgcgtcacc cgggaccata aacgtcacgc
      421 aagcggatcc gccgagaccg acggaggctc cccgacggca gattgcgagg gagcttgagc
      481 ggagccagga gcggagggag agggattcga accctcggta cctttcagta cacacgattt
      541 ccaatcgtgc accttcggcc actcggtcat ccctcctcgt gagggaggtg ccttgctgtc
      601 gctcggttcg tcctgacctg ctcccgcagg tccccgcccg cttcgcgggc ggcctgtcgc
      661 ttcgcgccag gggcgaattc tccgtgctcg gcggagcgca ttcaaacagg gcggaggggg
      721 agggatttga acccccggtg ctttcgcaca tctgatttcg aatcagacac cataggccac
      781 tcggacaccc ctccagaaac gccagaaacg agaaacagcg agaacagcgg cgtggaagcc
      841 tggccggcga gctgttcgct acgaatagct cggccctcgc cgcagctcgg cgaagaacgc
      901 ctggagcacg gccgcacact cggccgcgcg aacaccggcc gtgactgcga cgcgatgatt
      961 gagccgcgga tcggagacga tctggtacag cgtcgccacg gcgcccgcct tggggttcgg
     1021 acacccgaac accaggcgag cgacccgggc attgaccagt gcgccggcgc acatcggaca
     1081 cggctcctgg gtgaccacca gcgtcccttc cacccgccac tgccccagca ccgtggccgc
     1141 ctcgcgcagc gcgacgatct cggcatgcgc cgtggggtcg cgatcgacct cccggcggtt
     1201 gcggccgcgg ccgacgatgc gatcgccgac cacgatgacc gcgccgaccg gcacatcccc
     1261 cgacgccgcc gcggcccgcg cctcgtccag cgcgaggccc atccagtgct cgtcggtcat
     1321 gggacttctc tactacgtgc tgtgccggtg gcggtcgccg gtggcggtcg taaaccctgt
     1381 aacggcggat ccgagagggg tcgaacctct aacccccggt tccgtagacc ggtgctctat
     1441 ccattgagct acggatcctc atctttttca cgacagcggc agcccgcacc ggcagcccgc
     1501 agcggcggcc ccaatggcgg ataaaatggc ggagagagag ggattcgaac cctcggtaca
     1561 ggaaaaaccc catacgccgg tttagcaaac cggtgccttc agccactcgg ccatctctcc
     1621 aatctcccat ccccaacccg tagccacgac cccaccgatg ccaaacgctc gaaacgatca
     1681 gatcccgggc cagatcatcg gccagatcat cggccagatc atcggtaaga cgcgcggaac
     1741 gcccgcgtcc ggcccggaac cacttgcgtg acactattca gttatcaagc caaccagcgg
     1801 aggaggaggg attcgaaccc ccggagcttg cgctcggcgg ttttcaagac cgctgccttc
     1861 gaccactcgg ccactcctcc aggcgatccc agaggtaggg gcgcagtata gggatagccg
     1921 gtcgcttgta aagcctgatc tcggacaaac cgtggaccgg ccgggacctc gacccaggcc
     1981 tcgaccttga cggtcgtcgt tgaggttgac gtggccgtgg acgttgagcc catcatggac
     2041 ctcgacgtgg ccctccgttg caggtgcaag gtcaacgatg gggtcaacga tggggtcgac
     2101 gccaacgtcg ccgtcgatgt cgaggctctg gtcaaggtca tcgtcgagga caaggtcgaa
     2161 gaggtgagaa tcatcttccg ttcccgtctt ctttgatcga ccggccggga cctccggtca
     2221 gcggccggtc gagacccgct ccaggcctcc ggtgtagggc cgcagcgcgg ccggcaccag
     2281 gatcgagcca tcggcctgct ggtactgctc gaagatcgcc acgagcgtgc ggccgagcgg
     2341 cagcccggac ccgttgagcg tgtggaccgg gcgcggcttg tcgccgggcg cggggcggta
     2401 gcggatcttg gcgcgccggg cctggaagtc cgtgcagttc gagcacgacg agatctcgcg
     2461 gtacgcgtcc tggcccggca gccagacctc gatgtcgtag gtcttcgccg cgctgaaccc
     2521 caggtctccc gtgcacaggg tgatcacgcg gtagtgcagc cccaggccct gcagcacgcg
     2581 ctcggcgtcg cgcaccatcg cctcgagctc gtcgtagctg gtgtccggcg tcgtgatctt
     2641 gaccagctcg accttgtcga actggtgctg gcggatcagg ccgcgggtgt cgcggcccgc
     2701 cgcgccggcc tcggaccgga agcacgccgc gtaggcgcag tgcctccgcg gcagggtctc
     2761 gccgtcgagg atctgatcca tgtacaggtt ggtcatctgg atctcggcgg tcgacgacag
     2821 gaacaggtcg ttgtcggcgt cgtgatcggg cccgaccggg atgtcgagcc ggaacacgtc
     2881 ctcggcgaac ttcggcagct ggccggtgcc gatcagggcc gcgcgccgga cgatcgccgg
     2941 cggccacgcc tcctggtagc cgtgctgggt gtggaggtcc atcatgtagt tgagcagcgc
     3001 gcggttgagc cgcgacgccg cgccgcgcag cacggtgaac cgcgcgccgg cgatccgcgc
     3061 gccgccctcg aagtcgaaca ccccgagcgc gcggccgagc tcgtcgtgcg gccgcggcgt
     3121 gaacgagaac gccggcttgt cgccccaggt gcgcaccacc gggttatcgg cctcgccggc
     3181 gccgtccggc acgctggcgt gcggcgcgtt ggggatcgtg agcaggtgct gctcgcacac
     3241 cgcctcgagc tgcgcgagct cgccctcgcc ctccttgatc cggcccgcga gcgtcttcat
     3301 gtcgtcgcgc gccgcggcga actcggccga cttcttgtcg agcttggcca tcttctcgtt
     3361 ggccgcgttg cgctgctggc gcagcgagtc gagctctccc tgcagcttgc ggcgacgctc
     3421 gtcggcgctc cgccacccgg ccaccagcgg ggcaacctcc gggccgcgtc gcaggacgtc
     3481 atccatccgg gcaggatcga gcatgcgtga ctccgttcct cgtttttgca atgatcgaga
     3541 gacgttggcg gagcacccta cctcccgctt aaccacgctg gcaagccggg ggtccccatc
     3601 cccgtggagt ctctcgcggc cggactgccc ctgctcgagc ggtccgccgc cgtgaccgcg
     3661 gacgacccgg accaggcgct cgcagagcgg ttccgcgatg gcgatcgcac cgcgttcgac
     3721 gccctggttc gccgccacca gaagggaatg tggcggctga tccggcgtta cgtcaagagc
     3781 gatgccgatg ccgccgatgt cacccagctc gcgttcgtcc gcgcgttccg cggcctggcg
     3841 acgttccgcg gcacggccac ggtgcgcagc tggctgtacc ggatcgcgat caactgcgcg
     3901 ctgtcgtggc tgcgcgatca ccggcgcgag cagcccgccg agatcggcga ggacgcgttg
     3961 accgacgaca accccgcccc ggcccagctc agcgccagcg acgaccgcgc ccggctacgc
     4021 gccgcgatcg cccagctgcc gcccaagcag aagctggtcc tcgagctccg cgtgttcgac
     4081 gacctggcgt tccgcgaggt cgccgatctc gcggactgca gcgagaacac cgccaaggtc
     4141 aacttccact acgccgtgaa gcggctgcgt gacatcctc
//
LOCUS       DASVOR010000459         3100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_62667,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000459 DASVOR010000000
VERSION     DASVOR010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62667"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VF516_09150"
     CDS             <1..552
                     /locus_tag="VF516_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107881.1"
                     /translation="NLCSALHKFFHDPAAYRDWRAGQPFNVCRWDRDMRWMSYRGIVP
                     DFVVVPDIVAGGLASLEWSAFWRDTVPEEFRAYLAVQDGMTAADVVPHLTRYHGILVG
                     GSLHWKLATGAVWAELARRHGLGCHIGRVGTAARVHWARSIGATSVDSSLPLRAREHL
                     DAFLAAVGPIRRASTTNGAPPPP"
     gene            1111..1854
                     /locus_tag="VF516_09155"
                     /pseudo
     CDS             1111..1854
                     /locus_tag="VF516_09155"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
     gene            2363..>3100
                     /locus_tag="VF516_09160"
     CDS             2363..>3100
                     /locus_tag="VF516_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8107882.1"
                     /translation="MGIDPGRQQPANPLAPSTRRAAKRSSASPSSRPSSPRPANTSSR
                     CRTSTRRPTRSSRRPTRKSSPPTRSCRASTKSSRPRRRSWSQRAQEAQASLAAIVSCS
                     DDAIVSTDLRGVVQTWNRGAQQLFGYTAEEAIGQPTTLLIPLDRIDEYPGILERVRRG
                     ETIDHYETVRQHKDGKRLDISLTISPIRDAGARIIGGAKIARDITERKRIERELAYYI
                     AELSAADLRKNEFLAMLGHELRNPLSAL"
BASE COUNT          560 a         1008 c         1018 g          514 t
ORIGIN      
        1 aacttatgct cagctctcca taagttcttc cacgacccgg ccgcctatcg cgactggcga
       61 gcgggacagc cgttcaacgt ctgccgctgg gatcgcgaca tgcggtggat gtcctatcgg
      121 ggcatcgtgc cggacttcgt ggtcgtcccg gacatcgtcg ccggcgggct cgcgtcgctc
      181 gaatggtcgg cgttctggcg tgacaccgtg cccgaggagt tccgggccta cctcgcggtg
      241 caagatggga tgacggcggc ggacgtggtc cctcacctga cgcgctatca cgggatcttg
      301 gtcggcggat cgctacactg gaagctcgcg accggcgccg tgtgggccga gctggcgcgt
      361 cggcatggcc tggggtgtca catcggtcgt gttgggaccg cggcccgcgt tcactgggcg
      421 cgctcgatcg gcgcgaccag cgttgattcg tctctgccgt tgcgcgctcg cgagcacctg
      481 gatgcgtttc ttgccgcggt tggaccgatc cggcgtgcct ccaccacgaa cggcgcaccg
      541 ccgccgccgt gatcgtcgcg gcggctgagc tgagcaccaa gtcggtgggc gagcggcacg
      601 gcgctggcat tcgccgcggc ggcgggtgag cggcgcagct gttgatcgcg gcggaggcga
      661 gcaccacttg gcgcggcgaa gggcatcagt ggccgcgagg ccgtggctct cgcgatcgcg
      721 gcgaagggca tcatcgagtc cgctggtgat ggcggttcag tccgagccga tcgccatggc
      781 ggtcgacttc ggcggcttgg gagtcaggcg acgcctgcga tcaagatcgc aacgggccct
      841 tcgagcccgg atagcacggc cacgtcgggt gactcggggt aggtccgcac agatcgagcc
      901 caagcgggcg ctgtagccct tagcccttat cgacctcgat gggttgcgcg cgcgttcacg
      961 acctcccgaa gcgtccataa cggcgcgccg gctgcatcga ggtcgattgt tccggcgtcg
     1021 cggcgatttc gagatgaggc cgagcgaggt ggcgagccaa cctccgccaa gagcgcgaag
     1081 gccgcgcgaa cagccgatgg ttaccagaag atcatcttgc tgctccacaa ccattccggc
     1141 gtagatttct cgctgtacaa atccacgacc atccgacggc gcatcatccg acgtctggtg
     1201 ctcaacaagc agaacacgct cgaggactac gccggcttcc ttcgcggcaa caccaaggag
     1261 ctcaatgccc tctactccga cgtcctgatc agcgtgacga gcttctttcg caatcccgag
     1321 accttcgagg tgctccggca caaggtcctg cccgagctgt tcgagcgcaa gggtgacgat
     1381 ccggtgcgat gctgggtgct cggctgctcc accgggcagg aggcgtactc gatcgcgatg
     1441 atgttcgtgg aggcggcgga gatggcatcc cgcgtcaagc ggcttcaggt cttcgccacc
     1501 gacctcaacg atgagctcct cgacaaggcc cgccacggtc tgtatgccaa gagcctggtc
     1561 gacgacctca cgccgcagcg gctgcagcgg ttttttgtcg aggaggacgg gggctaccgg
     1621 atcacgaagg cggtgcggga gatggtggtc ttcgcacggc agaacttgat cgccgacccg
     1681 ccgttctcgc gcatggacct catcagttgc cgaaacctcc tcatctacct cgaaccaagc
     1741 ttgcagctca aggcgatccc gacgtttcac tatgcgctca aacccaaagg gttcttgctg
     1801 ctcggcgcat ccgaatcgat cggcggcttc acagatctgt tcgagctggt cgacaagaag
     1861 cacaagatct attcgaggaa gccagcgccg accgccgcgg tacctctgcc ggttctcacg
     1921 cagcgcgagc gtctggccgg cccgcgtccg ccgctcccga tccagccgct cggtgcggcg
     1981 gaactgaccg gacatccgcg gggcgagctc gatgcccagc gcgaggccga ccggatcacg
     2041 gtgcaccagt tcgcaccacc ggcggtgctg gtgaatgccg agctgaccgt cctgcagttc
     2101 cgcgggccga ccagcgcgtt tctgcagccg ccggtcggca aggcgagctt caacgtgctg
     2161 aagatggcac gggacggttt gatgctgccg ctgcgcgccg cgatcaacca ggcaaaaaag
     2221 gatggcaaga cggcgcgcaa ggacaatgtt cgcgtccagc acgacggcca tacccgcacc
     2281 gtgagcctgg aagtgattcc gctgaagaac ctgcgcgagc gctgctttct gatcctgttc
     2341 gaggacgtgg aattggcgag cggtgggcat cgaccccggt cgacagcagc cggcgaaccc
     2401 gctcgcaccg tccacgcgac gcgcggcgaa acgcagcagc gcatcgccca gctcgagacc
     2461 gagctctccg agacccgcga atacctccag tcgctgcagg accagcacga ggcggccaac
     2521 gaggagctcc aggcggccca cgaggaagtc cagtccgcca acgaggagct gcagagcgtc
     2581 aacgaagagc tcgagacctc gaaggaggag ctggagtcag cgagcacagg aggcgcaggc
     2641 aagcttggcc gcgatcgtct cgtgttcgga cgatgcgatc gtcagcacgg accttcgagg
     2701 cgtcgtccag acctggaacc ggggtgcgca gcaactgttc gggtacacgg cggaagaagc
     2761 gatcggccag ccgaccacac tcctgatccc actagaccgc atcgatgaat accccgggat
     2821 cctggagcgc gttcgccgcg gcgaaacgat cgatcactac gagaccgtgc gccaacacaa
     2881 ggatggcaag cggctcgaca tctcgctgac catctcgccg attcgagacg ctggtgcacg
     2941 catcatcggg ggtgcgaaga tcgcccggga catcaccgag cgaaagcgga tcgaacgcga
     3001 gcttgcgtac tacatcgccg agctatccgc ggccgatctc cgcaagaacg agtttctggc
     3061 gatgcttggt cacgagcttc gcaatccgct gtccgcgctc
//
LOCUS       DASVOR010000460         6013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_471934,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000460 DASVOR010000000
VERSION     DASVOR010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6013
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471934"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VF516_09165"
     CDS             <1..295
                     /locus_tag="VF516_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107883.1"
                     /translation="AVAVDPRRWDACCNALTLLLDRGDPDALARAAALLTRVPPEVKG
                     TSPQLLFNEAVYLHRAGRSAEARANLERVLAATHGDGELGALARQLMTEVSDG"
     gene            288..575
                     /locus_tag="VF516_09170"
     CDS             288..575
                     /locus_tag="VF516_09170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107884.1"
                     /translation="MADNAPVVLDLPWGDSGTALGLTVTFNGAAPVHYGDGKPPKGSV
                     ILVLAHGDQEDTVVWMEDDWAEPHQAFGYTYRVLNPPELKPRSVRVEVRKP"
     gene            608..3703
                     /locus_tag="VF516_09175"
     CDS             608..3703
                     /locus_tag="VF516_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8107885.1"
                     /translation="MGSPHAGHYNATVDLLEWNLDEHRDHPYLITSNNTFSYGDVVER
                     ARTFGAGLLDRGLAHGDRVLIITKDCVEFVCTFWGAIRAGLVAVPISPAMMPAEFRGI
                     VDDSRARAVVFDSGSERLVAAAKVSEVLCIATEPSSIAGAIPWPDVAGAPERLRDVAT
                     CTSDIAFWLYTSGTTGTPKGVMHSHGNLQASRNGQAKQVLGMGPGDVILSVSRMHFSY
                     GLGNSVFVPAAVGASVIVNTAPAIPAALQALIDVYAPTLLYGVASFWAGYVGLPAANT
                     GPRMRAAISGGEVLRPDVLERFRARFGVPLLDSLGSTEAMYHVASNRLDDMVPGSVGR
                     PLRGFEAQIRDPDGNTVIGQRGELWLRGPHMFVGYWNRPDLTARTLVDGWMRTGDTAR
                     IVDDRIYHEGRLDDLMKLGGNWVAPREIEQEIAAHPDVADVAVAIVDNDTGVPILKAF
                     VRSRRHDPELSRELFDACSARLAAYKVPRAFEIVAEFPRTATGKLKRYLLRSGAASST
                     EGLLAQVWKERLSVEHVSRTDNFFALGGDSLAALTVLNKVSEQVGIPLPSSLMFQNAG
                     LAELAALIDRMVGVPGADSLRPRPERFGQFPTSDVQTRMWLVEQMDGRSNTLEALVLH
                     GTLDHEALERAWALLVARHPALQVTFHCEAMRTVVQNLEPRPTATVEHASLEHLPADQ
                     RAAECERIAAAARLHRFDLASWPLFSATLIRIDPREHVLVIALPHILTDRWSADILFR
                     DLAELYRAELAGTPPALPPLRLDHVDYVLWQRASTMQSQLGRHLEFWRTALAGAPEVL
                     HIPAAMAPPAAASQATSNVSFQLDDALVRRLREIARENSATFYHVVMTGFFALLHRYT
                     EAVDIVVGTSVHNRGDDSLSQVVGCFMDHVPVRAKLAKRISFARLLAEVRDFATSAFS
                     HYVPLGVLLDELAPTRHAEHDPVFQVTFNLVQRKPQSSFPGCRVERRVGSLEIDRLYL
                     NLNLLETDDHVVASLDYSATRFEPSMAEQLAAHYVVLIEAAIVDPNRPIEDLPMMQDD
                     Y"
     gene            complement(3739..4761)
                     /locus_tag="VF516_09180"
     CDS             complement(3739..4761)
                     /locus_tag="VF516_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107886.1"
                     /translation="MVDGTVVSPWEPGHEAMVPTQGLEEIRDYHLLYGGQPFTEEQLA
                     PARKEVEDFVHILEAEGVIVRRPDILDNSEPYSTPDFTSPSGTGQYNPRDVMTVIGQE
                     IIESNMSWRARFFEYRPYRRLVIDYFKRGAKWTASPKAMHTDDLYHPHHRRGPDWEPV
                     TTEVEPVWDAADLTRCGRDLFFQRSHTSNRFAIEWLRRHLGDEYRVHEVQFHDERAVH
                     IDATFVPLCPGKFLVNPDRPMKDGPVLEMLKKSRWEMLTCPRTTYPRDSKSFRSFEWL
                     LMNMLSLDHERILVEKQEEPLQRALREWGFKPIPVAYRNAYKHGGSFHCSTADIRRRS
                     KLESYF"
     gene            complement(4832..>6013)
                     /locus_tag="VF516_09185"
     CDS             complement(4832..>6013)
                     /locus_tag="VF516_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107887.1"
                     /translation="ISLGSLIAILVAAYLLLRRMGVQRSLSVCACTLTLGLYSQQLAL
                     IEVRGDLLPAALNLWGLVVFVSDRRKLGLTAALFTLAFLAKMTTVFGAVAVALSLLFA
                     GQRKAALRFVGWMALGCTAGLLVVHIASGGRALQAFTAVASGGASWVQILLRAPLLML
                     KIVLPSDLILAVLALATAAGLPGQERRDVLPLAVLTTGFITLVMYGSPGVDRNHLIDF
                     DFIAMVFLVASGARGRIPLAQAMTGLSLAVLIVVPTILRQPPPQRTTIEKVIAETRSG
                     SGPLLVENPWWAILAGERPFMLDAFNLRIMADRDPEVARALYTRLDTRFFRAVILEMP
                     IDQPEFIWGDQWYGDVHFGKEFPEHLTANYDLVTRYPGARGSSYFIWRPKASSTPKHG
                     E"
BASE COUNT         1062 a         1974 c         1924 g         1053 t
ORIGIN      
        1 cgcggtcgcc gtcgatccgc gccggtggga cgcgtgctgc aacgcgctga ccctgctgct
       61 cgatcgcggc gatccggacg cgctcgcgcg cgccgccgcg ctgctcaccc gggtcccgcc
      121 cgaggtcaag ggcacgtcgc cgcagctgct gttcaacgag gccgtctacc tccaccgcgc
      181 cggccgctcc gccgaggcgc gcgccaacct cgagcgcgtg ctggccgcga cccacggcga
      241 cggcgagctc ggcgcgctcg cccgccagct catgaccgag gtgtccgatg gctgacaacg
      301 ctcccgtcgt gctcgacctg ccgtggggcg actccggcac cgcgctcggc ctcaccgtca
      361 ccttcaacgg cgccgcgccg gtccactacg gcgacggcaa gccccccaag ggctcggtca
      421 tcctcgtgct cgcacacggc gaccaggagg acaccgtggt ctggatggag gacgactggg
      481 ccgagccgca ccaggcgttc ggctacacct accgcgtgct caacccgccc gagctcaagc
      541 cgcgctcggt gcgcgtcgag gttcgcaagc cgtagcggta aatcaagcag ggagtgatgt
      601 gaacgtcatg ggttccccgc acgctgggca ctataacgcc acggtcgatc tgctcgagtg
      661 gaatctcgac gagcaccgcg accatcccta tctgatcaca tcgaacaaca cgttctcgta
      721 cggcgacgtc gtcgagcgcg cgcggacatt cggtgccgga ctactagatc gtggattggc
      781 gcatggtgat cgcgtgctga tcatcacgaa ggactgcgtg gagttcgtgt gcaccttctg
      841 gggggcgatt cgagcgggac tggtggccgt accgatctct ccggcaatga tgccggccga
      901 gtttcgcggc atcgtcgacg acagccgagc cagagcggtc gtgttcgatt cgggcagcga
      961 gcgcctcgtc gccgcggcga aggtcagcga ggtcctgtgc atcgcgacgg agccatcgtc
     1021 gatcgctggt gccatcccct ggccggacgt cgccggggcg ccggagcgtc tgcgagatgt
     1081 cgcgacctgc acgagcgaca tcgcgttctg gctgtacacc tcgggcacga cgggaacacc
     1141 gaagggggtg atgcactcgc atggcaacct ccaggcatcg cggaacggcc aggcgaagca
     1201 ggtgctcggt atgggccccg gcgacgtgat cctgtcggtc tcgaggatgc atttctcgta
     1261 cggcctcggg aactcggtgt tcgtccccgc ggcggtgggg gcatcggtga tcgtgaacac
     1321 cgcgcctgca attccggctg ccctgcaggc gctgatcgac gtgtacgcgc ccaccttgct
     1381 atacggcgtc gcatcgttct gggccggcta tgtggggcta ccggcggcga acaccggacc
     1441 gcggatgcga gcggcgatct cgggtggcga ggtactccgg cccgatgtgc tcgagaggtt
     1501 tcgggctcgg ttcggtgtgc cgctcctgga tagccttggc tcgacggaag ccatgtacca
     1561 cgtcgcgtcg aaccggctgg acgacatggt ccccggcagc gtcggacgcc ctctgcgcgg
     1621 cttcgaggct cagatccgcg atcccgacgg aaacactgtg atcggccagc gcggcgaact
     1681 gtggcttcgg ggtccgcaca tgttcgtggg ctactggaac cgtcccgacc tgaccgcccg
     1741 cacgctcgtc gacggatgga tgcgcacggg cgacaccgcg cggatcgtcg acgatcgcat
     1801 ttatcacgag ggccgcctcg acgacctcat gaagctcggt ggcaactggg ttgctccgcg
     1861 cgagatcgag caggagatcg ccgcccatcc ggatgtggcg gacgtcgccg tcgcgatcgt
     1921 cgacaacgat accggagttc cgatcctgaa ggcgttcgtc cggtcccgcc gccacgaccc
     1981 ggagctctcg cgggagctgt tcgatgcatg cagtgcgcgg ctggcggcgt acaaggtgcc
     2041 ccgagcgttc gagatcgtcg cagagtttcc acggacggca acggggaagc tcaaacgcta
     2101 cttgctgagg tctggagctg cgtcgtcgac cgaaggcttg cttgcgcagg tatggaagga
     2161 gcggctctcg gtggagcatg tgagccgcac cgacaacttc ttcgcactcg gcggcgactc
     2221 cctcgccgca ctgacggtct tgaacaaggt gagcgagcaa gtcggcatcc cgctgccatc
     2281 gtcgctcatg ttccagaacg ccggcctggc cgagctcgct gcgctcatcg atcgaatggt
     2341 aggcgtgccg ggagcggatt cgttgcgacc gaggcccgag cggttcggac agtttcccac
     2401 gtccgacgtg caaactcgca tgtggctcgt cgagcagatg gacgggcgca gcaacacgct
     2461 cgaggcgctg gtgttgcacg gcacgctcga ccacgaggca ctcgagcgcg cctgggcgtt
     2521 gctcgtggcc cgccacccgg cgcttcaggt cacgttccat tgcgaggcga tgcgcaccgt
     2581 cgtacagaac ctcgagcccc gcccgacggc gaccgtcgag catgccagcc tcgagcacct
     2641 gcctgccgat caacgcgcgg cggaatgcga gcggatcgcc gccgctgcac ggctccaccg
     2701 cttcgacctc gccagctggc cgctgttctc ggccacgctg attcgcatcg atcctcggga
     2761 acatgtcctg gtcatcgcgc tgccgcatat cctgacggat cgctggtcgg ccgatatcct
     2821 gtttcgagat ctggcagagc tctatcgtgc cgagctggct ggtacgcctc ccgccctacc
     2881 cccgcttcga ctggaccacg tcgactacgt tctatggcag cgcgcctcaa cgatgcaatc
     2941 ccagctcggt cgccacctcg agttctggag aacggcactg gcaggcgccc cagaggtgct
     3001 acacatacca gcggcgatgg ccccgcctgc ggcggcgtcg caagcgacaa gcaatgtgtc
     3061 cttccagctc gacgacgctc tcgtccgccg tctgcgcgag atcgcgcgcg agaactccgc
     3121 aaccttttac cacgtcgtga tgacaggatt cttcgccctc ctgcatcgct acaccgaagc
     3181 cgtcgacatc gtcgtcggga cctctgtaca caatcggggt gacgactcgc tgtcgcaagt
     3241 cgtcgggtgc ttcatggatc atgtgcccgt gcgagcgaag ctcgcgaagc gcatctcgtt
     3301 cgccaggctc ctcgccgagg ttcgggactt cgcgacgtca gcattcagcc actacgtccc
     3361 cttgggcgta ctcctggacg agctcgcccc cacccgacat gccgagcatg atcctgtgtt
     3421 ccaggtgacg ttcaaccttg tccagcgcaa gccgcagtcc tcgtttccgg gatgccgcgt
     3481 cgaacggcgt gtcggtagcc tcgagatcga tcggctgtat ctcaacctca atctactgga
     3541 aaccgacgac catgttgtcg ctagcctgga ctactcggcg acccgcttcg agccatccat
     3601 ggccgagcag ctcgcggcac actacgtggt cctcatcgag gcggccatcg tcgatccgaa
     3661 ccgacccatc gaagacctac cgatgatgca ggacgactac tgactgcact tcgatcggat
     3721 ccggcaaacg cgtgcccctc agaagtacga ctcgagcttg ctccgccggc gaatgtcggc
     3781 cgtcgaacaa tggaagctac caccgtgctt gtaagcgttc cggtacgcga ccgggattgg
     3841 cttgaagccc cactcgcgga gcgcccgttg cagcggctcc tcctgcttct cgacgaggat
     3901 gcgctcgtgg tcgaggctca acatgttcat cagcagccac tcgaaactac ggaaactctt
     3961 cgagtcgcgt ggataggtcg tgcggggaca ggtgagcatt tcccagcgcg acttcttgag
     4021 catttcgaga accggtccat ctttcatggg gcggtccggg ttgacgagga acttgcccgg
     4081 acagagtggc acgaaggtcg cgtcgatgtg aaccgcgcgt tcgtcatgga actgtacctc
     4141 gtgcacgcga tactcgtcgc caaggtgacg tcggagccac tcgatggcga agcggttgct
     4201 cgtgtggctg cgctggaaga atagatcgcg cccacatcgt gtgaggtcgg ccgcgtccca
     4261 gactggctcg acctcggtcg tcacgggctc ccagtctggc ccgcgcctgt gatgcggatg
     4321 gtagaggtca tcggtgtgca tcgccttggg cgaagcagtc cacttggcgc cgcgcttgaa
     4381 gtagtcgatc acgagccggc gatacggtcg gtactcgaag aaccgggctc gccagctcat
     4441 gttcgactcg atgatctcct ggccgatcac ggtcatcaca tcgcgaggat tgtactgccc
     4501 ggtaccggac ggagatgtga agtccggagt cgaatacggc tcgctgttgt cgagtatgtc
     4561 cggtcggcga acgatgacgc cttcggcctc gaggatgtgg acgaagtcct ccacctcctt
     4621 gcgcgccggt gcgagctgct cctcggtgaa tggctgtcca ccatagagca ggtggtagtc
     4681 gcggatctcc tcgaggcctt gcgtcggaac catcgcttcg tgccctggct cccacggcga
     4741 aacgaccgtg ccatcgacca cgccgacgat gacctcttcg aggtgatccc attcattgtg
     4801 ggaattcacg actggaaggc tcgacattct actattctcc gtgtttagga gttgaagacg
     4861 ccttgggacg ccagatgaag taactactgc cgcgggctcc aggatacctc gtgacgaggt
     4921 cgtagttcgc ggtcagatgc tcggggaatt ccttgccgaa atggacgtct ccgtaccatt
     4981 gatctcccca gatgaattct ggctgatcga tcggcatctc caggatgaca gcacggaaga
     5041 atcgtgtatc gagacgcgtg tacagcgcac gagcgacctc cgggtctcgg tccgccatga
     5101 tgcgcaggtt gaaggcatcc agcatgaacg ggcgttcccc ggccaggatg gcccaccacg
     5161 gattctcgac gagcaggggg ccggagccgc ttcgggtctc cgcgatgacc ttctcgatcg
     5221 tggttcgctg cggtggcggc tgccgcagga tggttggcac cacgatgaga acggcgagcg
     5281 acaagcctgt catcgcctgg gctaacggga tccgtccacg agcaccggaa gcgaccagga
     5341 acaccatggc aatgaagtcg aagtcgatca agtgattccg atcaacaccc ggcgagccgt
     5401 acatgaccag cgtgatgaag ccggtcgtga gaaccgccag cggcagcacg tcccggcgtt
     5461 cctgtcccgg gagcccggcg gcggtagcca gcgcgagcac cgcgaggatc aagtcggacg
     5521 gcagcacgat cttgagcatc agcagcggag cgcgcaacag gatctggacc cacgacgcgc
     5581 cccccgaggc gaccgcggtg aacgcttgca gcgctcgccc gccgctcgcg atatggacaa
     5641 caagcagccc tgcggtgcag ccgagcgcca tccatccgac gaagcgcagg gcagccttgc
     5701 gctgaccggc gaacagcaac gacagcgcca cggcaaccgc gccgaagacg gtcgtcatct
     5761 ttgccaggaa ggcgagcgtg aacagcgccg cggtcagacc gagctttcgc cgatcggaga
     5821 cgaacaccac cagcccccac aagttcaacg ctgccgggag taggtctccg cgcacttcga
     5881 tgagcgcgag ctgttgcgaa tacagaccca gggtgagggt gcacgcacag acagacagcg
     5941 aacgttgcac gcccatgcgc cgcagcagca gataggctgc caccaggatc gcgatcagcg
     6001 agccgagcga gat
//
LOCUS       DASVOR010000461         6435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_33346,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000461 DASVOR010000000
VERSION     DASVOR010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6435
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33346"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2910
                     /locus_tag="VF516_09190"
     CDS             <1..2910
                     /locus_tag="VF516_09190"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8107888.1"
                     /translation="ATSSAAVLDQTQRYAKDSVIVRFRSAPTAPFARTLATRVNGAIE
                     DRDNDGIDDRLGHIANGKLAVVRLDGQMDVSAALDELRKDPTILYAEPNYILRTAAVP
                     DDARFPELYGLHNTGQTGGTPDADIDAPEAWDRTVGSSKVVIGVIDTGVDYRHEDLAA
                     NMWVNPREIPDNGIDDDGNGVIDDVHGFDAITGSGDPFDDNHHGTHTAGTIGAVGNNG
                     IGVVGVNWHTQIMALKFLNYDGWGTTADAIRAIDYAVAQKLAGVNLRVLSNSWGGDGF
                     SQALLDAILSAGEADILFVAAAGNSATDTDAFPYYPSAYEAPNLVSVAATDQADRLAS
                     FSNFGATTVDLGAPGTNILSTVPNNDYQFLDGTSMATPHVAGVAALALSINDTLTADE
                     LKDILLTSGDPIPALNGTTFSGRRLNAAAAVARAGRPVPRFTTSVVPSRMVISQGTSA
                     AYGIDVRSVLGFSGRVVLTVSSEPPIAAALSITPVVTAPGTGTLTVTTSQQTALGSYK
                     LTITGTSGGLAASRTVALGILAPGTRFELNVTPASQITQQTFGATFQVVLQGVGDFHG
                     EIGLSFTSQPPFPGTAYFEPGAVTAPGASTLHVDSDCTTPPGDYVFTMVGTAEGATAS
                     ATATVRLDRFGTFAMTAPSLDTPMAIPDASPAGITSTIEIAEDIPIDDLSVTVNITHT
                     RIGDLVVQLIGPNGTAVTLHDRTGGDGDDLHQTYTVRDFTGLSSNGLWRLQVSDRAAR
                     EIGSLDSWQLNLGWHPDTFPPFANFQFSVDGRTVDFLDTSFGTGCGGGAPIVSRSWDF
                     GDGATSTGPSTTVRHTYAAPGDYVVTLTVTDASGLTDSMQQTVTATRPPPVLSIVRVV
                     RDRTHHTLQVDLRWCGAQGRRVELHRNQALVSRPWNDGSYRDVFRAQEMAYRWYVCEQ
                     AGLCSNEVSIDLGPSPAGDQATMTTEIGGTQIVERIAVADERVTDRAMRCATHRS"
     gene            2889..3338
                     /locus_tag="VF516_09195"
     CDS             2889..3338
                     /locus_tag="VF516_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107889.1"
                     /translation="MRNAPVLISWRDVSAASASDPLRFLEAPGTALVRQNLAWLRTET
                     NPLPAHRARIAAARPRALFANFPIHRLPGALVACQHLAALGCKPVAVRGRLVPAEARG
                     DFAAISFADGLLPLLGDANQFDPDPWPRSVANVKTSSGLACRISLPR"
     gene            complement(3718..4455)
                     /locus_tag="VF516_09200"
     CDS             complement(3718..4455)
                     /locus_tag="VF516_09200"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8107890.1"
                     /translation="MSMVWLETPHELRRAMRVRSKGSIRIHCGNRVIPGRLVDLSVGG
                     ICMRTELPIGLIALIGELVRVDLRLDAGASRHFALLGRVLRVSLPTRRMAIGFDAVPD
                     DFEDCVQGELLAAVEHDILPRMILVDTVAGRRNKIASAFRNAGCHVTEASTPLEAIAY
                     LGGSRFELGLIAIADTTPETVAEQLREFLLDEHPGVHMVSIGRSAAHRDPAGSWLCST
                     NVRGDLQTRVGRVMTAHGRRRHPTMSS"
     gene            5233..>6435
                     /locus_tag="VF516_09205"
     CDS             5233..>6435
                     /locus_tag="VF516_09205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107891.1"
                     /translation="MRAELFSISQLERHARTIAGWHELTSGPGHDDWLLARLGDNEVA
                     LRDAYALVTDAVQRGRQITPAAEWFIDNYHLIEEQIRTARRHLPRAYHRELPRLGNGP
                     APGTPRVYHIALELISHAHGRVDAEGLRAFVASYQGIQPLRLGELWAIPIMLRLALLE
                     NLRRVAMTITAGRHDREAAANWVARMLTAAAANPTDVVLVLAELVEANPPFSNAFVAE
                     LASRVQAQGSALMFPMSWLEHRLAESGQTVEHVFELATQSQAADQVSIGNSIGSLRFL
                     GATDWRDFVEAMSVVERTLRSDPGYGAMDFATRDRYRRAVETIARRSTRSEDEVARAA
                     IRLASDGTGRAAHVGYFLIDGGRRQLERCVAMRRSFGQIVRRTGGALRHAIYAGALAL
                     VTAIAALGL"
BASE COUNT         1066 a         2247 c         2120 g         1002 t
ORIGIN      
        1 gcgacgagct cggccgccgt actcgaccag acgcagcgct acgcgaagga ctcggtcatc
       61 gtgcgcttcc ggtcagctcc caccgcgccg ttcgcacgca cgctcgccac gcgggtgaac
      121 ggtgccatcg aggacaggga caacgacggt atcgatgatc ggctcgggca cattgcgaat
      181 ggaaagcttg cggtcgtccg cctggatggc cagatggacg taagcgctgc gctcgacgag
      241 ctccgcaagg acccgaccat cctctacgcc gaacccaact atattctccg gaccgcggcg
      301 gtccccgatg acgcgcgatt tcccgagctg tacggcctcc acaacacggg ccagaccggc
      361 ggcaccccgg acgcagatat cgacgccccc gaggcatggg accgcacggt gggcagctcc
      421 aaggtcgtga tcggcgtgat cgataccggg gtcgactatc gccacgagga tctggcggcc
      481 aacatgtggg tcaacccgcg tgagatcccg gacaatggga tcgacgacga cggaaacggt
      541 gtgatcgacg acgtgcacgg gttcgatgcg atcaccggga gcggcgatcc gttcgacgac
      601 aaccaccacg gcacgcacac cgccggcacc atcggagccg tcggaaacaa cggcatcggt
      661 gtcgtgggcg tcaactggca cacccagatc atggcgctca agttcctgaa ctacgacggc
      721 tggggcacca ccgcagacgc catccgagcg atcgactatg cggtggcgca gaagctcgcg
      781 ggtgtgaatc tacgtgtcct gtccaacagc tggggtggcg acggcttcag tcaggcgctg
      841 ctcgacgcga tcctctccgc cggtgaggcc gatatcctgt tcgtcgccgc ggcgggcaac
      901 agcgccaccg acaccgatgc attcccgtac tacccgtcgg cgtacgaggc cccgaacctc
      961 gtgtcggttg ccgctaccga ccaggccgac cgacttgcgt cgttctcgaa cttcggcgcc
     1021 acgacggtcg atctcggcgc gccgggcacg aatattttgt cgacggtgcc gaacaacgat
     1081 tatcagtttc tggatgggac ctcgatggcg accccgcacg tggccggcgt cgctgccctg
     1141 gccctgtcga tcaacgacac gctcacagcg gacgagctca aggacatcct gctgacctcc
     1201 ggcgatccga tcccggcgct gaacggcacc acgttcagcg gtcgccgcct caatgccgcg
     1261 gcagccgtcg cccgggccgg gcggcccgtc ccgcgcttca ccacgagcgt cgtcccgtcg
     1321 aggatggtca tctcgcaggg aacgagcgcg gcgtacggca tcgatgtccg ttccgtgctc
     1381 ggcttctccg gacgcgtcgt gctcaccgtg agctcggagc cgccgatcgc ggcggccttg
     1441 tccatcacgc cggtcgtgac cgcgccgggc accggcaccc tgaccgtcac gacctcgcag
     1501 cagaccgcgc tcggatccta caagctcacc atcacgggca cgagcggcgg gctggccgcc
     1561 tcgcgcaccg tcgcgctcgg catccttgcg cccggcaccc ggttcgagct caacgtgacg
     1621 ccggccagcc agatcaccca gcagacgttc ggcgcaacgt tccaggtcgt cctccagggc
     1681 gtcggcgatt tccacggcga gatcggcctg agcttcacct cgcaaccgcc attccccgga
     1741 accgcctatt tcgagcccgg ggcggtgacc gctccaggag cctcgacgct gcacgtcgac
     1801 agcgattgca ccaccccgcc gggcgactac gtcttcacca tggtcggaac tgcggaggga
     1861 gcgactgcaa gcgcgaccgc caccgtgagg ctcgatcggt tcggcacctt cgccatgacg
     1921 gctcccagtc tcgatacccc gatggcgatc ccggacgcca gtccagccgg tatcaccagc
     1981 acgatcgaga tcgccgagga tatcccgatc gacgacctgt ccgtgaccgt gaacatcacg
     2041 cacacccgca tcggcgatct cgtcgtgcag ctgatcggcc ccaacggcac agcggtcacg
     2101 cttcacgatc gcacgggagg cgacggcgat gatctgcatc aaacctatac cgttcgggac
     2161 ttcaccggcc tgagctcgaa cggtctgtgg cggctccagg tctccgatcg tgcagcccgc
     2221 gagatcggca gcctcgacag ctggcagctg aacctgggct ggcatcccga cacgttcccg
     2281 ccgttcgcga acttccagtt ctccgtggac ggccggacgg tcgacttcct cgacaccagc
     2341 ttcgggaccg ggtgcggagg tggcgctccg atcgtctcgc gctcctggga cttcggcgat
     2401 ggtgcgacct cgaccgggcc ctcgacaacc gtgcggcaca cctacgcagc gcctggcgac
     2461 tatgtcgtca cgctgaccgt gaccgacgcc agtggcttga ccgattcgat gcagcagacc
     2521 gtcacggcca cccggccccc acccgtcctc tcgatcgtga gggtcgtccg cgaccggacg
     2581 caccatacgc tccaggtcga tctgcgctgg tgcggcgcgc aggggcgtcg ggtcgagctc
     2641 catcgcaacc aggcactggt gagcagacca tggaacgatg ggagctaccg tgacgtgttc
     2701 agagcgcagg agatggccta tcgctggtac gtctgcgagc aggctggcct gtgctcgaac
     2761 gaggtgagca tcgatctggg tcccagcccg gcgggcgatc aggcgacgat gacgaccgag
     2821 atcggaggca cgcagatcgt ggagaggatc gccgtcgcgg acgagagagt gactgaccgg
     2881 gcgatgcgat gcgcaacgca ccggtcctga tcagctggcg cgacgtgtcc gccgcgtccg
     2941 cctccgatcc gctgcgcttc ctcgaggcac cgggaacggc gctggtccgc cagaacctcg
     3001 cgtggctgcg caccgagacc aacccgctgc ccgcgcaccg ggcgcggatc gccgcggcac
     3061 ggccgcgcgc gctgtttgcg aactttccga tccaccggct ccccggggcg ctcgtggcct
     3121 gccagcacct cgccgcgctg gggtgcaagc cagtggcggt ccgcggtcga ctggtgcccg
     3181 ccgaggcgcg cggcgacttc gccgcgatca gcttcgccga cggcctgctg ccgctgctgg
     3241 gcgacgcgaa ccagttcgac cccgacccgt ggccccggag cgttgcgaac gtgaagacgt
     3301 cgtcgggctt ggcctgccgc atcagcttgc caaggtagta ggcgcggact tcgggatcgg
     3361 atctgccagc cggctgcgga agacgtcgag cgtgatgtcc tcgtcccaca gaaaatatgg
     3421 ccggcccttc gcatcgatca ggcggtcggc ggcgtggggc aagcaccgac gtggggtgcc
     3481 cagggccgga attgaaccag cgacacgcgg attttcagga gcgggcgccg cacgtgcaag
     3541 ctcgcgtatt cgtctggtct tcccgagagc tcggcgcgag cccgcggtcg tcggcaagac
     3601 gatttctgcc ggtggccgag cccgtcctga aggctcgtca gacccttgag tcccactcgg
     3661 gaccgatggt caccgacgag caggtcaggt cgctgaggca gaagctcatg atggcgctca
     3721 cgacgacatc gtcggatgcc ggcgcctgcc gtgtgcggtc atcacacgac cgacacgggt
     3781 ctgcagatcg ccgcgaacgt tcgtcgagca cagccagctc ccggcggggt cccggtgcgc
     3841 cgccgatctc ccgatcgaga ccatgtgcac gcccggatgc tcgtcgagca ggaactctcg
     3901 cagctgttcg gcgaccgtct ccggggtggt gtctgcgatc gcgatgaggc cgagctcgaa
     3961 gcgagatccg ccgaggtacg cgatcgcctc cagcggggtc gatgcctcgg tgacgtggca
     4021 gcccgcgttg cgaaatgcgc ttgcgatctt gttgcgtcgt ccagcgacgg tatccaccag
     4081 gatcatacgt ggcaggatgt cgtgctccac ggcggcgagc agctcgccct ggacgcagtc
     4141 ctcgaagtcg tcgggaacgg cgtcgaatcc gatcgccatg cgccgggtcg gaagactcac
     4201 ccggagcacg cgtccgagca atgcgaagtg cctggatgcg cccgcatcga gtctgagatc
     4261 gacgcggacc agctcgccga tcaaagcgat caggccgatc ggaagctctg tgcgcatgca
     4321 gatgccaccg accgacaggt cgaccaggcg acccgggatc acccgattgc cgcaatggat
     4381 ccggatcgaa cccttcgagc gcactcgcat tgctctccgc aactcgtgcg gtgtctccag
     4441 ccaaaccatc gacatacctc cagccgtgaa acccggcgag cgccgcacac gcgcggtacg
     4501 tcgctgattc ctgcgacacc atacgccacg cgagcgccat cgccaaccgg aatcgtcgcg
     4561 accacagctt cgtgtgtcgc cgatagaact cagggattgt tcgcgaatac gctacggaag
     4621 gcgtcgcagc tggctgggcc ataatcaaac tggtgttgct gcgttgacat ttggccttgc
     4681 caggagcatc aattcgcgtg tcggtcgagt ggttcacgcg gcaccagacg agatcatcgc
     4741 ggcgcgttgt tggtgctacc gcagccaccg gccccggcag ggaccacgag gttccggatg
     4801 aatcgcgcgt acgatgagct gagccatgaa gcggtggtcg gcgcatggct cgcgcgatcg
     4861 gtcgactacg actcgtccat cgagatcgag cggtgacgca cgctggacgg agcggctcca
     4921 agagcggctg ctgacggtgc cgcgggcccc gctgatcgaa ggcctgcggt atggcctgat
     4981 cgagctgctc accatcatcg gagcactgac ggcggaaatg ctccgcaatg agctccatgc
     5041 cgctctcgag gcggtcacca tgccctctgg tccgagatta cgtcgttcat ggtatatgga
     5101 ctcgccgctt gcgcgcaccg gacgcagctc tcggtgtttc tcgtgcagcg ccccctcgaa
     5161 ccatctcagg cccgcaagtt gaagcatcgg gagaccgacg gcgcggcagg cgcgcggctc
     5221 cacgaatcgc cgatgcgcgc ggagctgttc agtatcagcc agctcgagcg ccacgcacgc
     5281 acgatcgccg gctggcacga gctcacctcg gggccgggcc atgatgactg gctgctcgcg
     5341 cggctcggcg acaacgaggt cgccctgcgt gacgcgtacg ccctggtcac cgatgcggtg
     5401 cagcgcggcc gccagatcac gccggccgcc gagtggttca tcgacaacta ccacctgatc
     5461 gaggagcaga tccgcacggc gcgccggcac ctgccgcgcg cgtaccaccg cgagctgccg
     5521 cggctcggca acgggcccgc gcccggcacg ccgcgcgtct atcacatcgc gctcgagctg
     5581 atctcgcacg cgcatggccg ggtcgacgcc gaggggctgc gcgcgttcgt cgcgtcgtac
     5641 caggggatcc agccgttgcg gctcggcgag ctgtgggcga tcccgatcat gttgcggctg
     5701 gcgctgctcg agaacctgcg ccgcgtcgcc atgacgatca ccgccgggcg ccacgatcgc
     5761 gaggccgccg cgaactgggt cgcccggatg ttgaccgccg cggcggcgaa ccccacggat
     5821 gtggtgctcg tgctggccga gctggtcgag gcgaacccgc cgttcagcaa cgcgttcgtc
     5881 gccgagctcg cgagccgcgt gcaggcccag gggtccgcgc tgatgtttcc gatgtcgtgg
     5941 ctcgagcacc gcctcgccga gtccggtcag accgtcgagc acgtgttcga gctcgccacg
     6001 cagagccagg cagccgacca ggtctcgatc ggcaacagca tcggcagcct gcggttcctg
     6061 ggcgccaccg actggcgcga cttcgtcgag gcgatgagcg tggtcgagcg cacgctgcgc
     6121 agcgatccgg ggtacggtgc gatggacttc gcgacccgcg accgctatcg ccgcgccgtc
     6181 gaaaccatcg cgcggcgcag cacccggtcc gaggacgagg tcgcgcgcgc cgcgatccgg
     6241 ctcgcgagcg acggcaccgg ccgcgcggcg catgtcggct acttcctgat cgacgggggc
     6301 cgccggcagc tcgagcgctg cgtcgcgatg cggcgatcgt tcgggcagat cgtgcgccgc
     6361 accggcggcg cgctgcgcca cgccatctac gccggcgcgc tcgcgctcgt cacggcgatc
     6421 gccgcgctcg ggctc
//
LOCUS       DASVOR010000462         1926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501166,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000462 DASVOR010000000
VERSION     DASVOR010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501166"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VF516_09210"
     CDS             complement(<1..1242)
                     /locus_tag="VF516_09210"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107892.1"
                     /translation="MNVSRCFPASVADDFLHGRLTSEEFARAVKHASGCADCQHLLFA
                     AARPSAEPPAGSMDDIQERLTTWRAARRAPLMPAFPWHKGHAVDRYVIIRSVGGTEDG
                     VIYEAFDPEREHRVVVKQLDLHVADPATEVFVSLARRQCQLSHPALLQMLSVGVHDGF
                     VYLVYEFVKGTTLLDAGGEDPRQILALFAEAGRGLAAAHDAGIAHGCFAATSCVVSRD
                     RRLKVLDFAIGEARIHRVAATKSAHDNDWTTSSDQVNSEDSYIGYIPSPSRKRPPSGQ
                     FEAIILAAGPNALGPRIYAAPELVLGAPPSPKSDQFAFCAALYHRLYGRPPYTGETIA
                     MWLRELLTGRKIQTPPLPGVPPAVQAALLRGLERDPELRFDRMQTLLGKLGRPPVRTG
                     RNRTIAAVAIGAAVAAVGGIAI"
     gene            complement(1550..>1926)
                     /locus_tag="VF516_09215"
     CDS             complement(1550..>1926)
                     /locus_tag="VF516_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107893.1"
                     /translation="TYNWQRCEVYTYLGRPLDMVPALEKSVRDLPGEYDPRARLGWIY
                     LQAGKLPEAARWTDEALAMVYGPRKAKVLAQRVDIAAQQHDKAAEKRFLQQTVQLWES
                     LPPGQDNPEALAKAKQDLAQVD"
BASE COUNT          268 a          670 c          720 g          268 t
ORIGIN      
        1 gatcgcgatc ccgccgaccg cggcgaccgc ggcgccgatc gcgaccgcgg cgatcgtgcg
       61 gttgcggccg gtccgcaccg gcgggcggcc gagcttgccg agcagggtct gcatgcggtc
      121 gaaccgcagc tccggatcgc gctcgaggcc gcggagcagc gcggcctgca ccgccggcgg
      181 cacgcccggc agcggcggcg tctggatctt gcggccggtg agcagctcgc gcagccacat
      241 cgcgatcgtc tcgccggtgt agggcggccg gccgtacagc cggtggtaca gcgcggcgca
      301 gaacgcgaac tggtcggact tcggcgacgg cggcgcgccg agcaccagct cgggggcggc
      361 gtagatccgc ggcccgagcg cgttggggcc ggcggcgagg atgatcgcct cgaactggcc
      421 gctcggcggg cgcttccgcg acggcgacgg gatgtagccg atgtacgagt cctcgctgtt
      481 gacctggtcg ctgctggtcg tccagtcgtt gtcgtgggcg gacttggtcg cggcgacgcg
      541 gtggatgcgg gcctcgccga tcgcgaagtc gaggaccttg agccggcgat cgcgggacac
      601 cacgcagctg gtcgccgcga agcagccgtg cgcgatcccg gcgtcgtggg ccgccgcgag
      661 gccgcgcccg gcctcggcga acagcgcgag gatctggcgc ggatcctcgc cgccggcgtc
      721 gagcagcgtg gtgcccttga cgaactcgta gaccaggtag acgaagccgt cgtgcacgcc
      781 gaccgacagc atctggagca gcgcggggtg cgacagctgg cactgccgcc gcgccagcga
      841 aacgaacacc tcggtcgccg ggtcagcgac gtggaggtcg agctgcttga cgacgacccg
      901 atgctcgcgc tcggggtcga acgcctcgta gatcacgccg tcctcggtgc cgccgacgct
      961 gcggatgatc acgtagcggt cgaccgcatg gcccttgtgc cacgggaacg ccggcatgag
     1021 cggggcccgc cgcgccgcgc gccaggtcgt caggcgctcc tggatgtcgt ccatcgaccc
     1081 ggccgggggc tcggcgctcg gccgcgccgc ggcaaacagc aggtgctggc agtccgcgca
     1141 ccccgaggca tgcttcacgg cgcgggcgaa ctcctcgctg gtgagccggc cgtggaggaa
     1201 gtcatccgca acgctggcgg gaaagcaccg ggacacgttc accctcccag gactatcccc
     1261 cacctcatcg ccgcagatcg ataggcagtt tcgagtacgc gctgacaggc ggacgggtcg
     1321 gccggcccgc cgcgccccgc gccggcgtgg tctggaaccc tgcgagatcc aatgcgagaa
     1381 gccggcgggg cagccgggag tcctgcgacc gaggccaccc gccgctcgcg gcgcaggcgc
     1441 ccggcagcga agcgatttcg agcgcttgtt ggacaagctg gctcatcaca cttgaggcgt
     1501 aatgttgtca gcagactgaa acggccgcgc ggtgagccag ctacagcggt cagtcgacct
     1561 gcgcgaggtc ctgcttggcc ttggcgagcg cctcggggtt gtcctggccg ggcggcagcg
     1621 actcccagag ctgcacggtc tgctgcagga agcgcttctc ggccgccttg tcgtgctgct
     1681 gggcggcgat gtcgacccgc tgggcgagca ccttcgcctt gcgcgggccg tagaccatgg
     1741 cgagcgcctc gtcggtccac cgcgccgcct cgggcagctt gccggcctgc aggtagatcc
     1801 agcccagccg ggcgcgcggg tcgtactcgc cgggcaggtc gcgcaccgac ttctcgagcg
     1861 ccggcaccat gtcgagcggc cggccgaggt acgtgtagac ctcgcagcgc tgccagttgt
     1921 aggtta
//
LOCUS       DASVOR010000463         7735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_471936,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000463 DASVOR010000000
VERSION     DASVOR010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7735
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471936"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..628)
                     /locus_tag="VF516_09220"
     CDS             complement(77..628)
                     /locus_tag="VF516_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HEX8107894.1"
                     /translation="MNAEQFRKLGHALVDWIADYREGVAARPVMSPARPGEIKARLPR
                     TPPEHGGGAGAMIAHLDADVMPGITHWNHPGFFAYFPSITSYASILGDLACAGLGVQG
                     MSWQTSPAATEVEEVMMDWLRQMVGLTAAWTGVIQDTASTATLSALICARERLTDFAP
                     VGLQAPGGALTVYTSVEAHSSVE"
     gene            933..1481
                     /locus_tag="VF516_09225"
     CDS             933..1481
                     /locus_tag="VF516_09225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8107895.1"
                     /translation="MADPEAPEPNPPEDLRTVFAKVRPTLLRMAENMCRVRADAEELL
                     QETFLRATRHGIPPDVRNVAAWLTTMLKNAIVDHGRKMKRRPSHESITDDHHGLTQLE
                     PDGPEPEWSDITLADIRDALEAIEPVYREVYHLREFEELSYEQIAQQLGIPRVTVGTR
                     LNRARKRLREVLVDRSGIGSQP"
     gene            1778..2224
                     /locus_tag="VF516_09230"
     CDS             1778..2224
                     /locus_tag="VF516_09230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107896.1"
                     /translation="MAYASPLLAAAAAIPLWLAHRSDAEFELALAVDPNPASARGQTT
                     PSQRSVAAHTGDVMRSSVRGERYLAIRVYLDEHRLVSACPEDARCHDAGGELTLELPL
                     AVPGKYVIVGLGSSDPLAAPASTLEQTQIAARRAGVYTKIQRLDVE"
     gene            complement(2388..2972)
                     /locus_tag="VF516_09235"
     CDS             complement(2388..2972)
                     /locus_tag="VF516_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107897.1"
                     /translation="MTSNAKTFSTAQLHCTEKTLLLELDPPAKLPVDVQSPAPAPVMR
                     GELAMECTDPKCLPGHDPSMVVPDPQNIAARVIPPFQYDLPVLITQGWVLAATDAMGT
                     IPLPVLDGSYELCKDGECDLTICKRPPCKGPCENLFAWRVALRLPEKTRFYVYKIQDV
                     SDELASLRDKLIAIPVPLGQPENSAKTAPVDSPT"
     gene            complement(2988..5132)
                     /locus_tag="VF516_09240"
     CDS             complement(2988..5132)
                     /locus_tag="VF516_09240"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8107898.1"
                     /translation="MPDAAELCLPAYELTRDPTLGSHAARSLQRRPGAEPIIAWIANA
                     IGDSPAGADAWFALALAREYHHDARGALTALERAVAMRGARDAAGQLRDANGLIFHHL
                     RASEYQEAIDQAVIAHGLIGAVESREDRAGAYINIATTLVTLGNLSTTEEILDEARPL
                     IPATSRLYARLRQLDAFVERGRGHREMETIALDEAHAIAIRDGDRVEEWNTRFNLIDA
                     AIRNGALRDAEAWLALPVPPDASRYDRATVAHRRALLASARGDHAAAADLIERVLPDA
                     PDSWRPSLDTVYGRALARLGRASAEPVLIRAVEAVEHKRASLDSDTFKSWLLASQREP
                     FEDLFLLYVSAGRLRDALATVQRATARSILDGLLQASHDDPSAVRDIAAVGAWSNSMR
                     RLAHALRSSRETARPDMTALLDRLRGNHVITYFRAGGDLWAIAVAGDGALRVRKVGSA
                     ADLAARVAAWREHLDDPQLAGALGDALLPDELVPPDGVPLYIIQDEPIGDVAFAALRR
                     HGELVLDHHPVAYAPSAAVLSATRRSSSPVRPTVLGDPTDDLSQARQEAVEVARRLQV
                     TPRLGAEASRAAVFDAAGATLIHIAAHTTPTTAGAALRMSDGLLDAGAVIDHGVAAGA
                     IVLMTCSSAAVTSRDELAPLASAFVAAGAHTVIASRWTVPDETARRFALMFYQADGLA
                     DPVTAVAGAQRQLVKDVPVKYWATFTVVGGLP"
     gene            5535..6500
                     /locus_tag="VF516_09245"
     CDS             5535..6500
                     /locus_tag="VF516_09245"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8107899.1"
                     /translation="MKGILKTHIPRSLMLAAAALVLSGVDASAGAPMAKGPQAGWFRM
                     KVGDFEVTALSDGTADLPMDKLLTNTTPDKVNKALAEHFMKAPVETSVNAFVVNTGSK
                     LVMIDGGAGTLFGPTLGKVVASLKAAGYKPEQIDEIYITHMHADHVGGLAAKDKPVFP
                     NATIRAGKAEGDFWLTQANMDKAPDAMKDFFKGAMASMKPYAKKYKPIEGDNVELVPG
                     IHALAAPGHTPGHTIYEIESKGEKMVFCGDLMHVAAVQFADPSVTIQFDADPKAAAPQ
                     RQKLYADATAKGYFVAFTHVSFPGIGHIRKEGTGYEWIPVNYTNK"
     gene            complement(6543..7430)
                     /locus_tag="VF516_09250"
     CDS             complement(6543..7430)
                     /locus_tag="VF516_09250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HEX8107900.1"
                     /translation="MAHPILFDPVDSPYLVPFDGSFKLRKLQTNPDENPGKEANIAAL
                     GQAIDKLSKLQEKLYAHDRYSVLLIFQAMDAAGKDGTIRAVMTGINPQGCQVYAFKSP
                     NAEELDHDFLWRVQRCLPERGRIGIWNRSHYEEVLVVRVNPNYLDHQKLPRRPKDLAD
                     LWTERYESIAAAEKHWARNGCAILKFFLNVSQKEQHKRFLERVTDPEHFWKFSSADMA
                     ESKKWEAYMDAYQDALAATSKPWAPWYAIPADSKHCMRRAVADILVGTLKQLHLEYPS
                     LPARELTELERVKVELQAE"
     gene            7537..>7735
                     /locus_tag="VF516_09255"
     CDS             7537..>7735
                     /locus_tag="VF516_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107901.1"
                     /translation="MATPSDPKPALSYPEHDKLGPLGAKTSAIQDAAALRGALIRAFE
                     AAAQDARDAVAAIDKGSAPAAV"
BASE COUNT         1221 a         2723 c         2650 g         1141 t
ORIGIN      
        1 cgggccgcat cgcgcgcgcg gcgtcggtcg cgatcgcgcg caggtgcgcc ttgccgagcc
       61 cggcgagccg caccgcctac tcgaccgagc tgtgggcctc gaccgaggtg tagacggtca
      121 gcgcgccgcc cggcgcctgc aacccgaccg gcgcgaagtc ggtcagccgt tcgcgcgcgc
      181 agatcagcgc gctcagcgtc gccgtgctgg cggtgtcctg gatgacgccg gtccacgccg
      241 cggtcagccc gaccatctgg cgcagccagt ccatcatcac ctcctcgacc tcggtcgcgg
      301 ccggcgaggt ctgccagctc atgccctgga ccccgaggcc ggcgcacgcg aggtcgccga
      361 ggatcgacgc gtagctcgtg atcgacggga agtacgcgaa gaacccgggg tggttccagt
      421 gggtgatgcc gggcatgacg tcggcgtcga ggtgcgcgat catcgcgccg gcgccgccgc
      481 cgtgctcggg cggcgtgcgc ggcagccgcg ccttgatctc ccccggccgc gccgggctca
      541 tcaccggccg ggccgcgacg ccctcgcgat agtcggcgat ccagtcgacc agcgcgtggc
      601 cgagcttgcg aaattgctcc gcgttcatgg ccccgaacat acgacgcaag cgcgccgccg
      661 tggcggccgt ccaccgatgt ccgtcgccga cgctggtcac cggcccccac tccaaccccc
      721 gacacgcgac ctcaagcctt tgcgaggccc tcgaccggtg ccgccagcgt cgacgacgga
      781 ctccccttcc ccgaccaccg tcgtcaccgg gacgacgcgc gtcgcgcgca acattcccga
      841 gctttttttg cgggaatatt tggctcgccg cgtcgtcgtc ggaatgcccg gtgttgaccc
      901 gaccttcaga cacgtgccac ggtccccggc ggatggccga tccggaggct ccggagccga
      961 acccgcccga ggacctgagg accgtcttcg cgaaagttcg gccaacgctg ttgcgcatgg
     1021 cggagaacat gtgccgagtc cgtgccgacg ccgaggagtt actgcaggag acattcctgc
     1081 gcgcgacgcg ccatggcatc ccacccgacg tccgcaacgt cgcggcgtgg ctgaccacga
     1141 tgctgaagaa cgcgatcgtc gaccacggcc gcaaaatgaa acggcgcccg agtcatgaat
     1201 ccatcacaga cgaccaccat ggcctcaccc agctcgagcc cgatggcccc gagcccgagt
     1261 ggagcgatat caccctcgcc gacatccgcg acgcgctcga agccatcgag cccgtgtatc
     1321 gcgaggtcta ccacctccgc gagttcgagg agctctcgta cgagcagatc gcgcagcagc
     1381 tgggaatccc ccgcgtcacc gtcggaacgc ggctcaaccg cgcccgcaag cgactgcgcg
     1441 aggtcctggt cgaccggtct ggcatcggga gccagccatg actgagcggt gcgccgatct
     1501 cgacgagttc ttcgacggcg agctgccggc cgatcgggcg gacgcgttcc gggaccacct
     1561 ggtcacgtgt gggcgctgtc agcgtctgct ggaggggagg atgcaggagt gtgtggccat
     1621 ccgggcaccc gcagctcgcc tccgaaccgc atcggcgcct gtggcagctg cggcggcacg
     1681 gggggagccg cacgtggtgt cgagcatcgc ggcggcccga cgagcctgtg acgcgacgcc
     1741 cgaccgaccg gcgcgaacct ggagcttgcg ccgcgcgatg gcgtatgcgt caccactgct
     1801 ggccgccgcc gctgcgatcc cgctctggct ggcccatcgc tccgacgcgg agttcgagct
     1861 cgcgctcgcc gtcgatccca acccggcaag cgcgcgcggc cagacgactc cctcgcagcg
     1921 gagcgtcgcc gcacacaccg gcgatgtcat gcggtccagc gtccggggcg agcgctacct
     1981 ggcgatccgg gtctacctcg acgagcaccg gctggtctcg gcgtgcccgg aagacgcccg
     2041 gtgccacgac gccggtggtg agctcacgct ggagcttccg ctcgccgtcc cgggcaagta
     2101 cgtcatcgtc ggtctgggat ccagcgatcc gctggcggcg ccagcatcaa cactcgagca
     2161 gacgcagatc gccgcccggc gtgccggggt ctacacgaag atccagcgcc tcgacgtcga
     2221 gtgatcgcat ccgggccggg cgggctatcg cggaactccc gcgtggcgca cgtgcgcgga
     2281 gaaccaccag cggatccggc gtggagctca gcaatctggc cgcatgactg cacgaccacg
     2341 gaacggcaaa cctggccgcg gttcggccgg cttcgccgcg gttcggctca cgtcggcgag
     2401 tcgaccgggg cggttttcgc cgagttctcg ggctggccga ggggcaccgg gatcgcgatc
     2461 agcttgtcgc gcagcgacgc gagctcgtcg gagacgtctt ggatcttgta cacgtagaat
     2521 ctggtcttct cggggagcct caaggccacg cgccacgcga acagattctc gcatgggcct
     2581 ttgcacggcg gccgcttgca gatcgtcaga tcacattcac cgtctttaca cagctcgtac
     2641 gatccatcga ggacagggag cgggatcgtg cccatggcgt cggtggccgc cagcacccag
     2701 ccctgcgtga tcaggaccgg caggtcgtac tggaacggcg gaatcacgcg ggccgcgatg
     2761 ttctgcgggt ccggcacaac catgctcggg tcgtgccccg gcaggcattt cgggtccgtg
     2821 cattccatcg ccagctcgcc gcgcatcacg ggagccgggg caggtgattg gacgtccacg
     2881 gggagtttcg ccggagggtc tagttccagc aaaagtgtct tctcggtgca atgcagctgc
     2941 gcggtcgaaa acgtcttggc attcgaggtc atatcttctc caagggatca ggggaggcca
     3001 ccgacaacgg tgaaggtcgc ccagtacttg accgggacgt ccttcaccag ctgtcgttga
     3061 gcgccagcaa cggctgtgac gggatcggcc agaccatcgg cctgatagaa catcagggcg
     3121 aaccggcgag cagtctcgtc gggtacagtc cagcgcgatg cgatgacggt gtgtgcgccg
     3181 gcggcgacga acgccgaggc gagcggtgcg agctcatcgc ggctggtgac cgcagccgag
     3241 gagcaggtca tcaggacgat cgcgccggcc gcgacaccgt ggtcgatcac cgccccggcg
     3301 tcgagcagcc cgtcggacat ccgcagcgcg gcgccggcgg tggtcggcgt ggtgtgcgcg
     3361 gcgatgtgga tcagcgtcgc acctgcggcg tcgaacacgg cggcccggga cgcctccgca
     3421 ccgagccgcg gcgtcacctg cagccgccgg gcaacctcga ccgcctcctg ccgggcctgg
     3481 gacaggtcat cggtcggatc gccgagcacc gtcgggcgga ccggcgagga cgacctccgg
     3541 gtcgcggaca gcaccgccgc actcggcgca taggccaccg ggtggtggtc gagcacgagc
     3601 tcgccgtgcc ggcgcagcgc cgcgaacgcg acgtcgccga tcggctcatc ctggatgatg
     3661 tacagcggca ccccgtccgg cggcacgagc tcgtcgggca acagcgcatc gcccagggca
     3721 ccggcgagct gtggatcatc gaggtgctcg cgccaggccg cgacccgcgc cgcgagatcg
     3781 gcggcgctgc cgaccttgcg gacgcggagc gcgccgtcgc cggcgaccgc gattgcccac
     3841 agatcgccgc ccgcgcggaa gtacgtgatc acgtggttgc cgcgcaggcg atcgagcagg
     3901 gccgtcatgt cgggcctggc ggtctcgcgc gaggatcgca gcgcgtgcgc cagccggcgc
     3961 atgctgttgc tccacgctcc gaccgcggcg atgtcgcgga ccgcggacgg atcgtcgtgc
     4021 gacgcctgga gcaagccgtc gaggatcgag cgtgcggtcg cgcgctgcac ggtcgcgagc
     4081 gcgtcgcgca ggcgtccggc ggagacgtac agcaggaaca ggtcctcgaa cggctcccgc
     4141 tgggaggcga gcagccagct cttgaacgtg tcgctgtcga gcgacgcccg cttgtgttcg
     4201 acggcctcga ccgcccggat caggaccggc tccgccgatg ctcgcccgag ccgggccagc
     4261 gctcgaccgt acacggtgtc cagcgagggc cgccaggagt ccggagcgtc gggcagcacg
     4321 cgctcgatca ggtcggcggc ggccgcgtga tcgccgcgcg ccgacgcgag cagcgcgcga
     4381 cggtgcgcca ccgtcgcgcg gtcgtagcgg gatgcgtcgg gcggcaccgg gagcgcgagc
     4441 cacgcctcgg cgtcgcgcag cgcgccgttg cggatcgccg catcgatcag gttgaaccgc
     4501 gtgttccact cctccacgcg gtcgccgtcg cggatcgcga tcgcgtgggc ctcatcgagc
     4561 gcgatcgtct ccatctcgcg atggccacgg ccgcgctcga cgaacgcgtc gagctggcgc
     4621 agccgcgcgt acagccggct ggtcgccggg atcagcggcc tcgcctcgtc gaggatctcc
     4681 tcggtcgtcg acaggttgcc gagcgtcacc agcgtggtgg cgatgttgat gtaggcgcca
     4741 gcgcgatcct cgcgcgactc caccgcgccg atcaggccgt gcgcgatcac cgcctggtcg
     4801 atcgcctcct ggtactcgct cgcccgcagg tgatggaaga tcagcccatt cgcgtcgcgc
     4861 agctgccctg cggcgtcgcg cgccccgcgc atcgcgaccg cgcgctccag ggccgtcagc
     4921 gcaccccgcg cgtcgtggtg gtactccctg gcgagcgcga gggcgaacca ggcgtcagcg
     4981 cccgccggac tgtccccgat cgcgttcgcg atccacgcga tgatcggctc ggcgccgggg
     5041 cgcctctgca ggctgcgcgc agcatggctg ccgagcgtgg gatcccgcgt cagctcgtag
     5101 gccggcaggc aaagctcggc cgcatcgggc aagcgagcaa tcgcggcctg ccggcaacgc
     5161 tcggccagcg tgggcgactc caccgccgca catgcgcaaa tcatgcacag ccagagcgcc
     5221 agcccgccga tccgcccacc gcccaccatc gaaggtacag cgtacagggc agccgaacgc
     5281 tgagccacca cccgcccggt catgattctg tcaccgcgat agcaggcgat gcgaccggcg
     5341 cgatctcgac aggacgcgtg tcgattttcc gtgctgtacc acttcacgat ccccacgacg
     5401 tccggccggc agcgtattcc cgcggaacgc gggagcaggt cccgaccgcc agttcaccgc
     5461 tggcggacgg ccccggggcg cttcggtcgg tgcaacgttg cagcggccga tccactcatg
     5521 tacgttccgc cgccatgaaa ggcatcctga agactcatat tccccgtagc ttgatgctcg
     5581 ccgcggcggc gctcgtgctg tccggcgtgg acgccagcgc cggcgcgccg atggccaagg
     5641 gcccgcaggc cggctggttc cggatgaagg tcggtgactt cgaggtcacc gcgctgtccg
     5701 acggcacggc cgatctgccg atggacaagc tgttgaccaa caccacgccg gacaaggtga
     5761 acaaggcgct ggcggagcac ttcatgaagg cgccggtcga gacctcggtc aacgcgttcg
     5821 tggtcaacac cggcagcaag ctggtcatga tcgacggcgg cgcgggcacc ctgttcggac
     5881 cgacgctcgg caaggtggtc gccagcctca aggcggccgg ctacaagccc gagcagatcg
     5941 acgagatcta catcacccac atgcacgcgg accacgtcgg tgggctggcc gccaaggaca
     6001 agccggtgtt ccccaacgcg acgatccgcg ccggcaaggc cgagggcgac ttctggttga
     6061 cccaggccaa catggacaag gcgccggacg cgatgaagga cttcttcaag ggcgcgatgg
     6121 cgtcgatgaa gccgtacgcc aagaagtaca agccgatcga gggcgacaac gtcgagctgg
     6181 tgcccggcat ccacgcgctg gccgcccccg gccacacccc cggccacacg atctacgaga
     6241 tcgagagcaa gggcgagaag atggtgttct gcggcgacct gatgcacgtc gccgcggtcc
     6301 agttcgccga cccgtcggtg acgatccagt tcgacgccga tcccaaggcc gcggcgccgc
     6361 agcgccagaa gctgtacgcc gacgccaccg ccaagggcta cttcgtcgcg ttcacccacg
     6421 tctcgttccc cgggatcggc cacatccgca aggagggcac cggctacgag tggatcccgg
     6481 tcaactacac caacaagtga agctcggccg cgcgacgcgg caaggctccc atcgctcgct
     6541 cctcactcgg cctggagctc gaccttgacc cgctcgagct cggtcagctc gcgggccggc
     6601 aggctggggt actccaggtg cagctgcttg agcgtgccga cgaggatgtc ggcgaccgcg
     6661 cgccgcatgc agtgcttgga gtcggccggg atcgcgtacc acggcgccca cggcttgctg
     6721 gtcgcggcca gcgcgtcctg gtacgcgtcc atgtacgcct cccacttctt gctctcggcc
     6781 atgtcggccg agctgaactt ccagaagtgc tcggggtcgg tgacccgctc gaggaaccgc
     6841 ttgtgctgtt ccttttgcga gacattgagg aagaacttga ggatcgcgca gccgttgcgc
     6901 gcccagtgct tctcggccgc ggcgatcgac tcgtagcgct cggtccacag gtcggcgagg
     6961 tccttggggc ggcgcggcag cttctggtgg tcgaggtagt tcgggttgac gcgcaccacc
     7021 aggacttctt cgtagtgact gcggttccag atcccgatcc ggccgcgctc cggcaggcag
     7081 cgctggaccc gccacaggaa gtcgtggtcg agctcctcgg cgttcggcga cttgaacgcg
     7141 tagacctggc agccctgcgg gttgatcccg gtcatcacgg cgcggatcgt gccgtccttg
     7201 cccgcggcgt ccatcgcctg gaagatcaac aggacgctgt agcgatcgtg ggcgtagagc
     7261 ttctcctgaa gcttggacag cttgtcgatc gcctggccga gcgcggcgat gttggcctcc
     7321 ttgcccgggt tctcgtcggg attcgtctgc aatttgcgca gcttgaacga gccgtcgaac
     7381 ggcaccaggt acgggctatc gaccgggtcg aacaggatcg gatgagccat gaagcggcac
     7441 cataccgcag cgctgtgtga caacttggat agaacacctc gaaatgacga tgacgtggcc
     7501 gcaatccgga cgtcacattg tatggtgagc gacgccatgg ccacgccgtc agaccccaag
     7561 cccgcgctct cgtaccccga gcacgacaag ctcggaccgc tcggcgcgaa aacgtcagcg
     7621 atccaggacg ccgccgcgct gcgcggtgcg ctgatccgtg cgttcgaggc cgccgcccag
     7681 gacgcgcgcg acgcggtcgc cgcgatcgac aagggctcgg cgccggccgc ggtcc
//
LOCUS       DASVOR010000464         2280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501183,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000464 DASVOR010000000
VERSION     DASVOR010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501183"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VF516_09260"
     CDS             <1..476
                     /locus_tag="VF516_09260"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8107902.1"
                     /translation="YRLDDGVARERQVLAIDDAIRRLGRDRDSPPQILCGDFNATADS
                     DEIRFLRGLTTLGGRRTHFQDAWLRLHREPGPGDGPAEGITWSSENRFTRPLRSLDLD
                     RRIDYVFVTTRKKDGRGTVHDCRVVLTEREGLGEVAICASDHYGVCADVQVAAVP"
     gene            689..>2280
                     /locus_tag="VF516_09265"
     CDS             689..>2280
                     /locus_tag="VF516_09265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107903.1"
                     /translation="MSAPSEDPTVAFPATRCARARRRPSGTTAVPSVSPATAATTATV
                     NAPLEALERDEIRRTRWFCYVAFVISTVAGSSLGWLHGDPTARAIMLVAIAASASATV
                     FMVFRTRDPVQFRRPSTGLGWVVIAAAATTAIPFFGAFSPAPVVLVLGIYFTSLGKSP
                     RLAFVLYAVAAGVQGVVSVLAITGLTRDTGLVQAGYLDLRQQLIIQGLVQFVFAVTLV
                     IGRLSRRAQRMAVEELEHEVRLSAHREALLIEAREQLERALRPGQGRFTGQQIGTYLL
                     GSVLGRGAMGEVYEASGPAGPVAIKLLSQASLGNANHVMRFFRELRTAAAIESPNVVK
                     VLEVGERPVPYLVMERLEGRTLSDILRSRRAMPLADVVDLIHQVGAGITAAAVVGVVH
                     RDLKPQNVFRHGETWKVLDFGVARAADQGDTLTDGQIVGTPSYMAPEQASGGEVDHLT
                     DLYALAAIAYRALTGHAPHASGDLAETLYRVVHASPRRPGDLAPRLPHDIDLVLAIGL
                     AKRPADRFRSADDLARALSDALT"
BASE COUNT          296 a          840 c          808 g          336 t
ORIGIN      
        1 actaccggct cgacgacggc gtcgcgcgcg agcgccaggt gctcgcgatc gacgatgcga
       61 tccgccggct cggccgcgac cgcgacagcc cgccgcagat cctgtgcggc gacttcaacg
      121 ccaccgccga cagcgacgag atccggttcc tgcgtgggct gaccacgctg ggcggccgcc
      181 gcacccactt ccaggacgcg tggctgcgcc tgcatcgcga gcccggcccc ggcgacggtc
      241 cggccgaagg catcacgtgg tcgagcgaga accgcttcac ccggccgctg cgctcgctcg
      301 atctcgatcg aaggatcgac tacgtgttcg tcaccacccg caagaaggac ggccgcggca
      361 cggtccacga ctgccgcgtg gtgctgaccg agcgcgaggg gctcggcgag gtcgcgatct
      421 gtgccagcga ccactacggc gtgtgcgccg acgtgcaggt cgcggcggtg ccctgaattg
      481 cggcagtgcg agttgacgcg gcgcagcgcc gtgcgatcgc gccgtgggca tccaggtcct
      541 ccggttgcac gaaccgtgca gcgcgcgggt cgatggccag atctggagtg cgcgatcctg
      601 tatgactgct taatctgccg agctaccctg tgagcgcggg agtcgtttgt tcgtgcccgg
      661 ggaggcttcc gcgttaggct gtgcgcgcgt gagcgcaccg tcggaagatc ctaccgtcgc
      721 gttccccgcg acgcggtgcg ccagggcgcg ccgccgaccg agcgggacga cggccgtgcc
      781 gtcggtcagc ccggcgacgg cggcgaccac cgcgacggtc aacgcgccgc tcgaggcgct
      841 cgagcgcgac gagatccgcc gcacccgctg gttctgctac gtcgcgttcg tgatctcgac
      901 cgtcgcgggc agctcgctgg gctggctgca tggcgatccg acagcccgcg cgatcatgct
      961 ggtggcgatc gcggcctcgg cgtccgccac cgtgttcatg gtgttccgga cccgcgatcc
     1021 ggtgcagttc cggcggccga gcaccggcct gggctgggta gtgatcgcgg ccgcggcgac
     1081 cactgcgatc ccgttcttcg gcgcattctc gcccgcgccg gtcgtgctgg tgctcgggat
     1141 ctacttcacg agcctgggca agagcccgcg gctggccttc gtgctctacg ccgtcgcggc
     1201 cggcgtgcag ggcgtcgtca gcgtgctcgc gatcaccggc ctgacccgcg acaccgggct
     1261 ggtccaggcc ggctacctcg acctgcgcca gcagctgatc atccagggcc tggtccagtt
     1321 cgtgttcgcc gtgacgctcg tgatcggccg gctgtcgcgg cgcgcccagc ggatggcggt
     1381 cgaggagctc gagcacgagg tccggctgtc ggcccaccgc gaggcgctgc tgatcgaggc
     1441 ccgcgagcaa ctcgagcgcg ccctgcgccc cgggcagggc cggttcaccg gccagcagat
     1501 cggcacctac ctgctcggca gcgtgctcgg ccgcggcgcg atgggtgagg tctacgaggc
     1561 cagcgggccg gccgggccgg tcgcgatcaa gctcctgtcc caggcctcgc tgggcaacgc
     1621 caaccacgtg atgcggttct tccgcgagct gcgcaccgcc gcggcgatcg agtcgccgaa
     1681 cgtcgtcaag gtgctggagg tcggcgagcg cccggtgccg tacctggtga tggagcggct
     1741 cgagggccgg acgctgtcgg acatcctgcg ctcgcggcgc gcgatgccgc tcgccgacgt
     1801 cgtcgacctg atccaccagg tcggcgcggg catcaccgcg gccgcggtgg tcggcgtggt
     1861 ccaccgcgat ctcaagccgc agaacgtgtt ccgccacggc gagacctgga aagtcctcga
     1921 cttcggcgtc gcccgcgcgg ccgaccaggg cgacacgctc accgacggtc agatcgtcgg
     1981 cacgccgtcg tacatggcgc ccgagcaggc cagcggcggc gaggtcgatc acctgaccga
     2041 cctctacgcg ctcgcggcga tcgcgtaccg cgcgctgacc ggccacgccc cccacgcctc
     2101 gggcgatctc gccgagacgc tgtaccgcgt ggtccacgcg tcgccacgcc gtccgggcga
     2161 tctcgcaccc cggctgccgc acgacatcga cctcgtgctc gcgatcggcc tggccaagcg
     2221 ccccgccgat cgcttccgct cggcggacga cctcgcccga gcgctgtccg acgcgctgac
//
LOCUS       DASVOR010000465         4172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_384104,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000465 DASVOR010000000
VERSION     DASVOR010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384104"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2394)
                     /locus_tag="VF516_09270"
     CDS             complement(<1..2394)
                     /locus_tag="VF516_09270"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8107904.1"
                     /translation="MSPSRPFILRPVATSLLMAAILLVGLVAVTQLPISALPQVDYPT
                     IQVVTFYPGASPEVTATTVTAPLERQFGQMQGLSQMTSTSGDGVSLIVLQFALALSID
                     VAEQEVQAGINAAQRLLPPGLPAPPVYSKANPADPPVLTLAVTSAARPLSVVEDLVDT
                     RVTPKLSQLSGVGVVQINGGQKPAVRVQVNPAALSSYGIDLEAVRTALVQTSVNGAKG
                     SFDGPAQSYQIDANDQLRTSAEYGRVVVAYRNGAPVMLTDVAQVIDGVENAFQAAWMN
                     DTPAVIVNIQRQPGANTISVVDSIRGLLPALQTNLPADLRVAVLTDLTVAIKASVRAV
                     ALELVLTIALVVLVIFLFLRSVSATVIPSVAVPLSLVGTFAAMYALGYSIDNLSLMAF
                     TIATGFVVDDAIVMIENVSRHLEAGATPLDAALRGAQQIGFTIISLTVSLIAVLIPLL
                     FMGDVVGRLFREFAVTLAVTIAISAVVSLTLTPMMCARILRHRPQAEQGRFYRGSERA
                     FGAMIGWYGRTLRVVLRHRTLTLIVAAATLAVTIALYAVIPKGFLPVQDTGVIQGISQ
                     APPTVSFAAMAERQQQLARVILRDPAVASLSSFIGVDATNTALNSGRIAINLRPLAER
                     GASAIEIQRRLGAALREASGITLYMQPVQNITVETRPSRTRYQYALEAPDPGELARWT
                     ARLVARLAALPELADVATDQQPGGLAVLLTIDRPTASRVGLTPSAIENTLYDAFGQRQ
                     INVLYTQSNQYHVVLEAEPGFRRGPAQLANIYLQAALRPGGGAGSAAPAAASSAAAGT
                     "
     gene            complement(2391..3623)
                     /locus_tag="VF516_09275"
     CDS             complement(2391..3623)
                     /locus_tag="VF516_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8107905.1"
                     /translation="MPVEPPHTDGAAPPPRKPHRRRRFAIWVAVIAAFVAVFWWALRG
                     RSTRGSKRGAAPAPVAIRTAAATRSDLPIYRDAIGTVTPVATVSITSQVTGRVFAVHY
                     QEGQRVGRGELLVDIDPRPFAALLMQAQGTLERDLHVLGQARMDLDRYRAAWARKAIA
                     RQQLDDQEKIVRQAEGTVKLDRGTVAYNRVQLAYCRIASPISGRVGLRLVDSGNLVTA
                     NAGPVLAVVTQLQPITVVFAISEDYLGEVRAQPDRGAGLPVEVLDRVQARRLAIGQLI
                     TIDNQIDTTTGTIRLRARFDNADETLFPNQFVNARLLVTTLRGVTAVPSAAIQRNGEI
                     AFVYLVAGGRVHIQRVTPGAVAGELTQVTGVAPGTVVAISSFEKLRDGAPVVIAEPQA
                     GSAGSASSAGSAGSGVPR"
     gene            complement(3701..>4172)
                     /locus_tag="VF516_09280"
     CDS             complement(3701..>4172)
                     /locus_tag="VF516_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107906.1"
                     /translation="AVGDVPVARLDPAAVAAAVKAGHSFFTTGPILDAAIAGKGFGDV
                     ATVSPGKVALHVAVRAANWISVHQLTVLGPGNTVLATRAIPSSTRPVRFDDTIELDVA
                     RDGYAIVRVDGDRPMAPNVGDLQSFLVYPVAIANPIWIDADGDGKVTPVAPYPH"
BASE COUNT          591 a         1500 c         1531 g          550 t
ORIGIN      
        1 cgtccccgcg gccgcggagc tcgccgcggc gggagccgcc gagccggcgc cgccccccgg
       61 ccgaagggcg gcctggagat agatgttcgc gagctgcgcc gggccgcgcc ggaaccccgg
      121 ctcggcctcg agcaccacgt ggtactggtt ggactgcgtg tacagcacgt tgatctggcg
      181 ctggccgaac gcgtcgtaca gcgtgttctc gatcgccgac ggggtcagcc cgacccgcga
      241 cgccgtcggg cgatcgatcg tcagcaggac cgcgagcccg ccgggctgct gatcggtggc
      301 gacgtccgcc agctcgggca acgcggcgag ccgcgcgacc aggcgcgccg tccagcgcgc
      361 gagctcgccg gggtccggcg cctccagcgc gtactggtac cgggtgcggc tcggccgggt
      421 ctcgacggtg atgttctgga ccggctgcat gtacagggtg atgccagagg cctcgcgcag
      481 cgcggccccg aggcggcgct ggatctcgat cgccgacgcg ccgcgctcgg ccagcggccg
      541 caggttgatc gcgatccgcc cgctgttcag cgcggtgttg gtcgcgtcga cgccgatgaa
      601 cgacgacagg ctggcgaccg cggggtcgcg caggatcacg cgggcgagct gctgctggcg
      661 ctcggccatc gccgcgaacg acacggtcgg cggggcctgc gagatgccct ggatcacgcc
      721 ggtgtcctgc accggcagga agcccttcgg gatcaccgcg tagagcgcga tggtcacggc
      781 cagcgtcgcc gcggcgacga tcagcgtgag cgtccggtgg cgcagcacga cccgcagcgt
      841 gcggccgtac cagccgatca tcgccccgaa cgcgcgctcc gacccgcggt agaaccggcc
      901 ctgctcggcc tggggccggt ggcgcaggat ccgcgcgcac atcatcggcg tcagggtcag
      961 cgacaccacc gccgagatcg cgatcgtcac cgcgagggtg accgcgaact cgcggaacag
     1021 ccggccgacc acgtcgccca tgaacaagag cgggatcagc accgcgatca gcgacacggt
     1081 cagcgagatg atcgtgaacc cgatctgctg cgcgccgcgc agcgcggcgt cgagcggcgt
     1141 cgcgccggcc tcgaggtggc gcgacacgtt ctcgatcatc acgatcgcgt cgtcgaccac
     1201 gaacccggtc gcgatcgtga acgccatcag cgacaggttg tcgatgctgt agccgagcgc
     1261 gtacatcgcg gcgaacgtgc cgaccagcga cagcgggacc gcgacgctcg ggatcaccgt
     1321 ggccgacacg ctgcgcagga acaggaagat caccagcacg accagcgcga tggtcagcac
     1381 gagctcgagc gcgaccgccc ggaccgaggc cttgatcgcg accgtgaggt cggtcagcac
     1441 cgcgacgcgg aggtccgcgg gcaggttggt ctgcagggcc ggcaacagcc cgcggatgct
     1501 gtcgaccacg ctgatcgtgt tggcgccggg ctggcgctgg atgttgacga tcaccgccgg
     1561 cgtgtcgttc atccacgccg cctggaacgc gttctcgacc ccgtcgatca cctgcgcgac
     1621 gtcggtcagc atcaccggcg cgccgttgcg gtaggccacg accacgcgcc cgtactcggc
     1681 gctggtccgc agctggtcgt tggcgtcgat ctggtagctc tgggccggcc cgtcgaagct
     1741 gcccttcgcg ccgttgacgc tggtctggac cagcgcggtg cgcaccgcct cgaggtcgat
     1801 gccatacgac gacagcgccg ccgggttgac ctggacccgc acggcgggct tctggccgcc
     1861 gttgatctgc acgacgccga cgccggacag ctgcgacagc ttcggcgtga cccgggtgtc
     1921 gaccaggtcc tcgaccacgg acagcggccg cgcggccgac gtcaccgcca gcgtgagcac
     1981 cggcggatcc gccgggttcg ccttgctgta gaccggcggg gccggcaggc cgggcggcag
     2041 gagccgctgc gctgcgttga tcccggcctg gacctcctgc tcggcgacgt cgatgctcag
     2101 cgccagcgcg aactgcagca cgatcagcga gacgccgtcg ccgctggtcg aggtcatctg
     2161 cgacaggccc tgcatctggc cgaactggcg ctccagcggc gcggtcaccg tcgtcgccgt
     2221 cacctccgga ctggcgcccg gatagaacgt gacgacctgg atcgtcgggt agtcgacctg
     2281 cggcagcgcc gagatcggca gctgggtgac cgcgaccagc ccaacgagca ggatcgcggc
     2341 catcagcagc gaggtcgcca ccgggcgcag gatgaacgga cgcgacgggc tcatcgcggc
     2401 accccggagc ccgccgagcc cgccgagctt gccgagcccg ccgacccggc ctgcggctcc
     2461 gcgatcacga ccggcgcgcc gtcgcgcagc ttctcgaagc tgctgatcgc gaccacggtc
     2521 cccggcgcga cgccggtgac ctgggtcagc tcgcccgcca ccgcccccgg cgtcacccgc
     2581 tggatgtgga cgcggccgcc cgcgaccagg tacacgaacg cgatctcgcc gttgcgctgg
     2641 atcgccgccg acggcaccgc ggtgaccccg cgcagcgtgg tgaccagcag ccgggcattg
     2701 acgaactggt tggggaacag cgtctcgtcg gcgttgtcga accgcgcgcg cagccggatc
     2761 gtgccggtcg tggtgtcgat ctggttgtcg atcgtgatca gctggccgat cgccagccgc
     2821 cgggcctgca cgcggtcgag cacctcgacc ggcagccccg cgccgcggtc gggctgcgca
     2881 cggacctcgc cgaggtagtc ctcggagatc gcgaacacga cggtgatcgg ctggagctgc
     2941 gtgaccaccg cgagcacggg gccggcgttg gcggtgacca ggttgccgga gtcgacgagc
     3001 cgcaggccga cccgcccgga gatcggggac gcgatccggc agtaggcgag ctggacgcgg
     3061 ttgtacgcga ccgtgccgcg gtcgagcttc acggtgccct cggcctgccg cacgatcttc
     3121 tcctgatcgt cgagctgctg gcgggcgatc gccttgcggg cccacgccgc gcggtagcgg
     3181 tcgaggtcca tccgcgcctg gcccaggacg tggaggtcgc gctcgagcgt gccctgggcc
     3241 tgcatcagca gcgccgcgaa cggccgcgga tcgatgtcga cgagcagctc gccgcggccc
     3301 acgcgctggc cttcctggta gtgcacggcg aacacccgtc cggtgacctg gctcgtgatc
     3361 gagaccgtgg cgaccggcgt caccgtgccg atcgcgtcgc ggtagatcgg cagatcgctg
     3421 cgcgtcgccg ccgcggtgcg gatcgcgacg ggcgccggag ccgcaccgcg cttgctcccc
     3481 cgcgtcgacc gtccgcgcag cgcccaccag aacaccgcga cgaacgccgc gatgaccgcg
     3541 acccagatcg cgaaccggcg gcggcgatgc ggcttgcggg gcggcggggc tgccccgtcc
     3601 gtgtgcggtg gctcgaccgg catggagggc acggtgctgc accgatcgcg gatgcaacgc
     3661 cggtacccgt gctcgacgag gacagcgcgc ccgcgcccgc tcagtgcggg tacggcgcga
     3721 cgggcgtgac cttgccgtcg ccgtcggcgt cgatccagat cgggttggcg atcgcgaccg
     3781 ggtagaccag gaagctctgg aggtcgccga cgttgggcgc catcggccgg tccccgtcga
     3841 cccggacgat cgcgtagccg tcgcgcgcga cgtcgagctc gatcgtgtcg tcgaaccgga
     3901 ccgggcgggt cgacgacggg atcgcgcggg tcgccagcac ggtgttgccc ggaccgagca
     3961 cggtgagctg gtgaaccgag atccagttcg cggcgcgcac cgcgacgtgg agcgcgacct
     4021 tgccgggcga gacggtcgcg acgtcgccga agcccttgcc ggcgatcgcg gcgtcgagga
     4081 tcggcccggt ggtgaagaag ctgtgtccgg ccttgaccgc cgccgcgacc gccgccggat
     4141 cgagccgggc gaccgggacg tcgccgaccg cg
//
LOCUS       DASVOR010000466         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_384116,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000466 DASVOR010000000
VERSION     DASVOR010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384116"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="VF516_09285"
     CDS             <1..1037
                     /locus_tag="VF516_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107907.1"
                     /translation="SATFFIGVQRMAHSKWWSPIRRVPEAFSLLLPVAAVLMIVLAFG
                     FHKLYPWTNPASPDMAAKDELVHAGRFTYLSPPFIYARMIVIIGLWIVFALRMRQISL
                     AGDASREAGNSAYSRLFRYSGVFVPLFAITFALSAYDWIISLEPKWFSTMFSVYIFAG
                     SFVETIAAIALATVILKRKQLWGKDGALVDMHVIRTLGTMMLAFSTFWGYTWVCQYLL
                     IWYSNIPDEAVWYVQRSSGGWLPLLIAGFAVSWVIPFFTLLPIKSKNSLKVMTVMAVL
                     ILLGRWFDLYMQVMPSHWDAPRFGWMELGMAAGTAGLIWLVFVRGLSRAPLIPVHEPV
                     LEARRGHAHH"
     gene            1050..>2455
                     /locus_tag="VF516_09290"
     CDS             1050..>2455
                     /locus_tag="VF516_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107908.1"
                     /translation="MKLVKLGVFVATGLLVGFLAKQPEPVLGDLPNRMPEAAPYITDE
                     AKMFPPPYEEFWKTQNHPGQCQTCHQKIFDEWNGSMMSNSWRDPVWRGAYLLLARTTS
                     TNGECDTPAPPDGTEKSHHNPFAKPGECASVFDIGTERVTLSRPGSLLDAFCSRCHMP
                     TNYLDNIPLRNITMDKFGLEHAPADLHFNPTSDNHTGIAFATVEEQFRNTESGKTGIF
                     CAICHSFAATRDTPFHNYTRSGKEYVPATGAESRADLVSADQDLFKVADLTRPNLGYS
                     IGAGAYRLSPHAIVFPERFGPMSATQAPEPSDTNTSKVFGEDIAYQQVDSSKHNAYHS
                     SMFVRSEMCAACHDVTNALPIRNTLGRFAGGFPIERTYTEWSNSRYAERPGNKFYEPA
                     HKRDCQSCHMQQDYGQPGTAQTLYDPAGHPLPILKEPVATDGKERPFFSHHFVGGNAF
                     VPRLIGKDVDNSGNVSPYP"
BASE COUNT          399 a          873 c          770 g          413 t
ORIGIN      
        1 tgtcggcgac gttcttcatc ggcgtgcagc ggatggccca ctcgaagtgg tggtcgccga
       61 tccgccgcgt gcccgaggcg ttctcgctgc tcctgccggt cgcggcggtg ctgatgatcg
      121 tcctcgcgtt cggcttccac aagctgtacc cgtggaccaa cccggcttcg ccggacatgg
      181 ccgcgaagga tgagctggtc cacgccggcc ggttcaccta cctgtcgccg ccgttcatct
      241 acgcccggat gatcgtgatc atcgggctgt ggatcgtgtt cgcgctgcgg atgcgccaga
      301 tctcgctggc cggcgacgcc agccgcgagg cggggaactc ggcgtacagc cggctgttcc
      361 gctattccgg cgtgttcgtc ccgctgttcg cgatcacctt cgcgctgtcg gcctatgact
      421 ggatcatctc gctcgagccc aagtggttca gcacgatgtt ctcggtctac atcttcgccg
      481 gctcgttcgt cgagacgatc gccgcgatcg cgctcgccac cgtcatcctc aagcgcaagc
      541 agctgtgggg caaggacggc gcgctggtcg acatgcacgt gatccggacc ctgggcacga
      601 tgatgctcgc gttctcgacg ttctggggct acacgtgggt ctgccagtac ctcctgatct
      661 ggtacagcaa catccccgac gaggcggtct ggtacgtcca gcgcagcagc ggcggctggc
      721 tgccgctctt gatcgccggc ttcgcggtca gctgggtcat cccgttcttc accctgttgc
      781 cgatcaagtc caagaacagc ctgaaggtga tgaccgtgat ggcggtgctg atcctgctcg
      841 gccggtggtt cgatctctac atgcaggtga tgccgtcgca ctgggacgcg ccgcggttcg
      901 ggtggatgga gctcgggatg gcggcgggca ccgccggctt gatctggctg gtcttcgttc
      961 gtggtctttc gcgcgcgccg ctgatcccgg tccacgagcc ggtgctcgag gcgcgccgcg
     1021 gtcatgcgca tcactaaagg aggagcgccg tgaaactcgt caagttggga gtgttcgtcg
     1081 ccacggggct cctggtcgga ttcctggcca agcagcccga gccggtgctc ggcgacctgc
     1141 cgaaccgcat gcccgaggcc gcgccgtaca tcacggacga ggccaagatg ttcccgccgc
     1201 cctacgagga gttctggaag acccagaacc acccgggcca gtgccagacc tgccaccaga
     1261 agatcttcga cgagtggaac ggctccatga tgtcgaactc gtggcgcgat ccggtgtggc
     1321 gcggcgcgta cctgctgctg gcgcgcacga cgtcgaccaa cggcgagtgc gacaccccgg
     1381 cgccgcccga cggcaccgag aagtcgcacc acaacccgtt cgccaagccc ggcgagtgcg
     1441 cgtcggtgtt cgacatcggc accgagcgcg tgacgctgtc gcgccccggc tcgctgctcg
     1501 acgcgttctg ctcgcgctgc cacatgccga ccaactacct cgacaacatt ccgctgcgca
     1561 acatcacgat ggacaagttc ggcctggagc acgcaccggc cgacctgcac ttcaacccga
     1621 cgtcggacaa ccacaccggc atcgcgttcg cgaccgtcga ggagcagttc cgcaacaccg
     1681 agtccggcaa gaccggcatc ttctgcgcga tctgccacag cttcgccgcg acccgcgaca
     1741 cgccgttcca caactacacg cgcagcggca aggagtacgt ccccgcgacc ggcgccgagt
     1801 cgcgcgccga cctggtctcc gccgatcagg acctgttcaa ggtcgccgac ctcaccaggc
     1861 ccaacctcgg ctacagcatc ggcgccggtg cgtaccgcct gtcgccgcac gcgatcgtgt
     1921 tccccgagcg gttcggcccg atgtccgcca cccaggcgcc ggagccgagc gacaccaaca
     1981 ccagcaaggt gttcggcgag gacatcgcct accagcaggt cgactcgtcc aagcacaacg
     2041 cctaccactc gtcgatgttc gtgcgctccg agatgtgcgc ggcgtgccac gacgtgacca
     2101 acgcgctgcc gatccgcaac acgctcggcc ggttcgccgg cgggttcccg atcgagcgca
     2161 cctacaccga gtggtccaac agccggtacg ccgagcgccc gggcaacaag ttctacgaac
     2221 cggcgcacaa gcgcgattgc cagtcgtgcc acatgcagca ggactacggt cagcccggca
     2281 cggcgcagac cctgtacgac ccggccggcc acccgctgcc gatcctcaag gagccggtcg
     2341 ccaccgacgg caaggagcgg ccgttcttca gccaccactt cgttggcggc aacgcgttcg
     2401 tgccgcggct gatcggcaag gacgtcgaca acagcggcaa cgtgtcgccc taccc
//
LOCUS       DASVOR010000467         2011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501218,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000467 DASVOR010000000
VERSION     DASVOR010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501218"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VF516_09295"
     CDS             complement(<1..209)
                     /locus_tag="VF516_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107909.1"
                     /translation="MRWDSPLVLALAGAFAVHLAAWTAGDVLVVTHPPEPHIPAPHID
                     LVEVEVPPVVKPPPPPPPEPEPPPEP"
     gene            complement(233..649)
                     /locus_tag="VF516_09300"
     CDS             complement(233..649)
                     /locus_tag="VF516_09300"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8107910.1"
                     /translation="MAGSSLYQDDDGSGAITDINVTPFVDVVLVLLVVFIVTAKLIVA
                     RGVEIDKPKAATGGDVQSTLRVSVDKDGQLFVNGDRFVSDPTAVARIKEIAATTTKPK
                     AIIAGDRATAYAGVMHAIDLVQQAGVTAIALENERP"
     gene            complement(636..1310)
                     /locus_tag="VF516_09305"
     CDS             complement(636..1310)
                     /locus_tag="VF516_09305"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8107911.1"
                     /translation="MDLSSVFVDFAQLGANWVLWLLIVLSVISVGVMIDRALWLRRRD
                     TDTERFTRELTGAFERGEIERVQTKYMDDPAIPIQVGLRGLAIRDKGAEAVAEAMHGE
                     RARWRRAADRNLIVLGTLGNNVPFVGLFGTVLGVINAFHHLATKAANAEQETLSNIAE
                     ALSATAIGLLVAIPAVIAFNFFSRRVRVIMGGADEVAHAVLSLVHGAERGGRAVAYKE
                     AGDGGE"
     gene            complement(1322..>2011)
                     /gene="truD"
                     /locus_tag="VF516_09310"
     CDS             complement(1322..>2011)
                     /gene="truD"
                     /locus_tag="VF516_09310"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEX8107912.1"
                     /translation="RFVLRVRDVAEDAEHKARAILAALAAPPGAPNWYGEQRFGRDGD
                     NAARGRALVTGERPLGRDRRLDRLMISALQSQLFNDWLTARIGDGLYRAVLAGDVLHK
                     RGGGMFVCDDPATDQARLAAGDLVVTGPMFGDRMRMPGTASPALAREAEVLARAGIPR
                     EAFSPQRVRALAEGTRRDAAVEVAGATVAPVDGSTIEIAFALPGGAYATAVMREVMKS
                     DQVVDAGPPAA"
BASE COUNT          285 a          738 c          712 g          276 t
ORIGIN      
        1 ggctccgggg gcggctcggg ctccggcggc gggggtggcg gcggcttgac caccggcggc
       61 acctcgacct cgaccagatc gatgtgcggc gcagggatgt gcggctcggg cgggtgggtc
      121 accaccagca catcgccggc ggtccaggcc gccaggtgga ccgcgaacgc gccggcgagg
      181 gcgagcacga gcggcgagtc ccagcgcacg gtgcaagcct agccgagccc ggctacgggc
      241 gctcgttctc gagcgcgatc gcggtcacac cggcctgctg caccagatcg atggcgtgca
      301 tcacacccgc gtacgcggtg gcgcgatcgc cggcgatgat cgccttgggc ttggtcgtcg
      361 tcgccgcgat ctccttgatc cgggcgaccg cggtggggtc gctcacgaac cggtcgccgt
      421 tgacgaacag ctggccgtcc ttgtcgaccg acacccgcag cgtgctctgg acgtcaccgc
      481 cggtcgccgc cttgggcttg tcgatctcga ccccgcgcgc gacgatcagc ttggcggtca
      541 cgatgaacac caccagcagc acgagcacga cgtcgacgaa cggcgtgacg ttgatatcgg
      601 tgatcgcgcc ggagccgtca tcgtcctgat acaggctact ccccgccatc gccggcctcc
      661 ttgtaggcca ccgcgcggcc gccgcgctcg gcgccgtgga ccagcgacag cacggcgtgg
      721 gcgacctcgt cggcgccgcc catgatcacg cggaccctgc ggctgaagaa gttgaacgcg
      781 atcaccgcgg ggatcgcgac caacaggccg atcgcggtcg cgctcagcgc ctcggcgatg
      841 ttcgacagcg tctcctgctc ggcgttggcc gccttggtcg ccaggtggtg gaacgcgttg
      901 atcacgccga gcacggtgcc gaacagcccg acgaacggca cgttgttgcc cagcgtgccg
      961 agcacgatca ggttgcgatc ggcggcgcgg cgccagcggg cgcgctcgcc gtgcatcgcc
     1021 tcggcgaccg cctcggcgcc cttgtcgcgg atcgcgagcc cgcgcagccc gacctggatc
     1081 gggatcgccg ggtcgtccat gtacttggtc tgcacccgct cgatctcgcc gcgctcgaac
     1141 gcgcccgtga gctcgcgggt gaatcgctcg gtgtcggtgt cgcggcggcg cagccacagc
     1201 gcgcgatcga tcatcacgcc caccgagatc acggagagaa cgatcagcag ccacagcacc
     1261 cagttggcgc cgagctgcgc gaagtcgacg aacacgctgg acaggtccat cgcgccgagt
     1321 gttacgcagc cggggggccg gcgtcgacca cctggtcgct cttcatgacc tcgcgcatga
     1381 ccgccgtcgc ataggcgccg ccgggcaacg cgaacgcgat ctcgatggtc gaaccgtcca
     1441 cgggagcgac cgtggcgccg gcgacctcga ccgccgcgtc gcgccgtgtg ccctcggcga
     1501 gcgcgcgcac gcgctgcggc gagaacgcct cgcgtgggat gccggcgcgg gcgagcacct
     1561 cggcctcgcg cgcgagcgcc gggctggcag ttcctggcat ccgcatgcgg tcgccgaaca
     1621 tcggcccggt caccacgaga tcgccggcgg cgagccgcgc ctggtcggtc gccgggtcgt
     1681 cgcacacgaa catcccgccg ccgcgcttgt gcagcacgtc accggcgagc accgcgcgat
     1741 acaggccatc gccgatccgc gcggtcagcc agtcgttgaa cagctgcgat tgcagcgcgg
     1801 agatcatcaa tcgatcgagc cggcgatcgc ggccgagcgg tcgctcgccg gtgaccagcg
     1861 cccggccgcg cgccgcgttg tcgccgtccc ggccgaaccg ctgctcgccg taccagttcg
     1921 gtgcgccggg cggcgccgcg agcgcggcga ggatcgcgcg cgccttgtgc tcggcatcct
     1981 ccgcgacgtc gcgcacgcgc agcacgaagc g
//
LOCUS       DASVOR010000468         1696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_237917,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000468 DASVOR010000000
VERSION     DASVOR010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237917"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..669
                     /locus_tag="VF516_09315"
     CDS             205..669
                     /locus_tag="VF516_09315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107913.1"
                     /translation="MRWATLVLLVLVACSDLRDFHGQWVGPRVGDARPLDVNIPDGKA
                     TLSIDQIDSHGLAARLAVDGLLPETAFTSIPGAEADVLSGITFSGAPLRVYLAFVPVP
                     DGRGDALAIIALYDDHRVEVRLLRGGSAPLYGIYTLTEVTAVTAPGTPTARS"
     gene            666..>1696
                     /locus_tag="VF516_09320"
     CDS             666..>1696
                     /locus_tag="VF516_09320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8107914.1"
                     /translation="MTGPGAPRGVICPRCGTANAGGARFCGVCGAAMASTEPATRSGA
                     APGDPMIGREIAGRYRVLAKLGEGGMGAVFRAEQISLKRTVAVKLLRPDVSAAPTLLR
                     RFNAEAELVAKLSHPNTVNIYDFGQERDGTLFIAMELIDGRSLRQAIQREAPMPLPRA
                     LAIAAQIAASLADAHGHAIIHRDLKPDNVMLQDRGRDRDVVRVLDFGIAKLRDDTRAT
                     HAAMTQQGDMLGTPQYMAPEQIRAEPIDGRTDIYALGCLLYEMVTARLPHQAETVLAM
                     LSKHLMEPAVPPSQRRPDLGIPAAADALILGAMAKDPRARPPSMEAFGEQIAALRQRL
                     GGEPGAPQA"
BASE COUNT          219 a          608 c          646 g          223 t
ORIGIN      
        1 gggtgggctc ggccgccggt ggcgggtcgt acggggcgtg tggctcgcgg gtcccgcgct
       61 gctccggctg ctcgggcggg tccggcggcg gctgcgcggc cagcgcgggc cgcgcgagga
      121 cgatcgccac cagcaagagc gccatccggg ccagcacggg tcagaacgta gcgcatggtt
      181 cgccggcggt gctaccgtga ggtcgtgcgc tgggccacgc tggtgttgct ggtgctcgtc
      241 gcgtgcagcg acctgcgaga cttccacggc cagtgggtgg gcccgcgggt cggcgatgcc
      301 cgaccgctcg acgtcaacat cccggacggc aaggcgaccc tgtcgatcga ccagatcgac
      361 agccatggcc tggcggcgcg gctcgcggtc gatggcctgc tgccggagac ggcgttcacg
      421 tcgatcccgg gcgccgaggc cgacgtcctg tcggggatca cgttcagcgg cgcgccgctg
      481 cgggtctacc tcgcgttcgt gccggttccg gatggcaggg gggacgccct ggcgatcatc
      541 gcgctgtacg acgaccaccg ggtcgaggtg cggctgctac gcggcggatc ggcgccgctc
      601 tacggcatct acacgctgac cgaggtgacc gcggtcaccg cgcctggcac gccgacggcg
      661 aggtcatgac cgggccgggc gcgccccgcg gtgtgatctg cccccggtgc ggaaccgcca
      721 acgccggcgg cgcgcggttc tgcggcgtgt gcggcgcggc gatggcgtcg accgagccgg
      781 cgacccggtc gggcgcggcg cccggcgatc cgatgatcgg ccgcgagatc gcggggcgct
      841 accgcgtgct cgccaagctc ggcgagggcg gtatgggcgc ggtgtttcgc gccgagcaga
      901 tctcgctcaa gcgcacggtc gcggtgaagc tgctgcggcc cgacgtctcg gcggcgccga
      961 cgctgctgcg ccggttcaac gccgaggccg agctggtcgc caagctcagc catcccaaca
     1021 ccgtcaacat ctacgacttc ggccaggagc gcgacggcac gctgttcatc gcgatggagc
     1081 tgatcgacgg ccggtcgctg cgccaggcga tccagcgcga ggcgccgatg ccgctgcccc
     1141 gcgcgctggc gatcgcggcg cagatcgcgg cgtcgctggc cgacgcgcat ggccacgcca
     1201 tcatccaccg cgacctcaag cccgacaacg tgatgctgca ggaccgcggc cgcgatcgcg
     1261 acgtggtccg ggtgctcgac ttcgggatcg ccaagctgcg cgacgacacc cgcgcgaccc
     1321 acgccgcgat gacccagcag ggcgacatgc tcggcacgcc gcagtacatg gcgcccgagc
     1381 agatccgcgc cgagccgatc gacggccgga ccgacatcta cgcgctgggc tgcctgctct
     1441 acgagatggt gacggcgcgg ctgccgcacc aggccgagac cgtgctcgcg atgctgtcca
     1501 agcacctgat ggagccggcg gtgccgccgt cgcagcgccg gccggacctc gggatccccg
     1561 cggcggccga cgcgctgatc ctcggggcga tggccaagga cccgcgtgcg cggccgccct
     1621 ccatggaggc gttcggcgag cagatcgcgg cgctgcgcca gcgcctgggc ggtgagccgg
     1681 gcgcgccgca ggcgtt
//
LOCUS       DASVOR010000469         3125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_179820,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000469 DASVOR010000000
VERSION     DASVOR010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179820"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VF516_09325"
     CDS             <1..257
                     /locus_tag="VF516_09325"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8107915.1"
                     /translation="CVHAARGRFDVDLSLAAIEVSFGAALVRVHRNWLVNIEHVLELE
                     GTASDAALLVGDDLRVPVARERAAAVRELLLANSAGVRRR"
     gene            complement(258..743)
                     /locus_tag="VF516_09330"
     CDS             complement(258..743)
                     /locus_tag="VF516_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107916.1"
                     /translation="MRTLLPVLIACSHASAPAPPPAAPTDHVVPPIPSVGENTMGWPT
                     WSHEKKLAYMKQTVMPAEREIFSRFEPVRFAFLTCETCHGQSARDGNYKMPNPDLPHL
                     VGGKQGFMELAEKQPETLRFMQSVVVPETARLLGYPAFDMEKHVGFSCYQCHVRADVK
                     Q"
     gene            1140..1721
                     /locus_tag="VF516_09335"
     CDS             1140..1721
                     /locus_tag="VF516_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8107917.1"
                     /translation="MATVAAALYGGRSAAIGLLIAMVALVVVTLRSRAGLALMAIRDA
                     EDAAAGLGVHVWRLKLLVFVVAAAGTGLCVALITVQTPFIQPNAAFSIQWSAAVSFMA
                     VVGGLGTVEGPLVGAAIYVVLQDQLRATGEVHLIVLGAVSVIVMLVAPRGIAGFVRDR
                     FGWTLFPVARRPPHAGPRPRSDGCSASLPGRSG"
     gene            1990..2190
                     /locus_tag="VF516_09340"
     CDS             1990..2190
                     /locus_tag="VF516_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107918.1"
                     /translation="MIFLGCMPLIMHAPPYYSGHAAFLMLATLWLVLVAVVAGLARLI
                     IRLLGGAASRDEGGSNAAPVDR"
     gene            2242..2808
                     /locus_tag="VF516_09345"
     CDS             2242..2808
                     /locus_tag="VF516_09345"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8107919.1"
                     /translation="MVAPSRHYTFEDYLSVEEMSAVRHEFLNGEIFAMAGGTPEHAAL
                     SAAAVLVLGRRLEGQPCRPYSADLRIRVVATGLATYADTAVICGDPIRDPASPTHVTN
                     PIVIVEVLSKSTEAYDRGEKREHYQQIASLREYVLVAQDKREIEVYARPTGGIWQRSV
                     YRAAETVDLPSIGIRFTVDELYSAAGVP"
BASE COUNT          471 a         1052 c         1064 g          538 t
ORIGIN      
        1 cctgcgtgca cgcggcgcgc ggtcggttcg acgtcgatct ctccctggcc gccatcgagg
       61 tgtcgttcgg tgctgcgctc gtgcgcgtgc atcgcaactg gctggtcaac atcgagcacg
      121 tgctcgaact cgaaggcacc gcgagcgacg ccgcgctgct cgtgggcgac gacctgcgcg
      181 tgccggtggc acgcgagcgc gctgccgcgg tgcgcgagct gctcctcgcg aacagcgccg
      241 gtgtgcgtcg gcgctgatca ctgcttcaca tccgcgcgga cgtgacactg atagcagctg
      301 aacccgacgt gcttctccat gtcgaacgcc gggtagccga gcaggcgcgc ggtctcgggc
      361 acgacaaccg actgcatgaa gcgcagtgtc tcgggctgct tctcggcgag ctccatgaag
      421 ccctgctttc cgccgacgag gtgcggtaga tccgggttcg gcatcttgta attgccgtcg
      481 cgcgcgctct ggccgtggca cgtctcgcac gtgaggaacg cgaagcgcac cggctcgaag
      541 cgcgagaaga tctccctctc ggccggcatc acggtctgct tcatgtacgc gagcttcttc
      601 tcgtgcgacc acgtcggcca gcccatggtg ttctcgccga cggacggaat cggcggcacg
      661 acgtggtcgg tgggtgcggc cggcggcggc gccggagcac tggcatgact gcatgcaatg
      721 aggacaggca agagcgttcg catgcctcgc agcgtaggcg cgccggcgtg cggcagcgag
      781 ggcgcgccga cgagccgtcg ctgcgtgccg acgagccgtc aacgcgcagt tcgacgattg
      841 agttggccat cagcccgcat cctcctcgtc atcgcgcgac ttgcgggcgc gcggcagcta
      901 caaccaatca gcggcacgcg gtttcaaccc gagcccgcgt caccaagcaa acgagggcac
      961 ggaacggcgt atgcacctaa gcgcatgctc cacggtgcac caagggcaca gggggcggag
     1021 caccgagcgt gacccaccac ggacctggcc cgtactacga agtgccgctc tggccacccg
     1081 tcaccgccac cctacccggt ggctggagca ccgagcgtat ccggatccgg gcaggcgctt
     1141 tggcgaccgt agccgctgcc ctgtacggtg gccggagcgc cgcgatcggc ctgctgatcg
     1201 ccatggtcgc gctggtcgtc gtcacgctgc gctctcgcgc gggcctcgcg ctgatggcga
     1261 tccgcgacgc cgaggacgcc gccgccggcc tgggcgtcca cgtctggcgg ctcaagctgc
     1321 tggtgttcgt cgtcgccgcc gcgggcaccg gcctgtgcgt cgcgctgatc accgtgcaga
     1381 ccccgttcat ccagcccaac gccgcgttct cgatccagtg gtcggcggcg gtcagcttca
     1441 tggccgtggt cggcggcctg ggcacggtcg agggccccct ggtcggcgcc gcgatctacg
     1501 tggtgctgca ggaccagctc cgcgcgaccg gcgaggtcca cctcatcgtg ctcggcgccg
     1561 tctcggtgat cgtcatgctc gtggcgccgc gcggcatcgc cggcttcgtc cgcgatcgct
     1621 tcggctggac gctgttcccc gtcgcccgcc ggccaccgca tgccgggcca cggccccggt
     1681 ccgatggctg ctcagctagt ttgcctggca ggtctggttg aagcaggccg aggcgcagaa
     1741 gctgcagctc tgtcccgtgg cgccagcgat ctggccgagt tgctggatat ctagctgacg
     1801 cagcgtctcg aggttcagct tgagggtccc agatttgcga agtgaatgct tgggcttcat
     1861 atatgctcct tgattcggga aatgttcaat tcgcgcgttg cgatagttct cgaattgagg
     1921 cgatgctaac atggatcacc acggtcacgg ctccctcgcg gctgcgccgc ggccgccgcg
     1981 atcttcgggg tgatctttct cggctgcatg ccgctgatca tgcacgcgcc gccctactac
     2041 agcggccatg ccgcgttcct catgctggcc acgctgtggc tcgtgctggt cgcggtggtc
     2101 gcaggactcg cccggctgat catccggctg ctcggcggcg ccgcgtcgcg cgacgagggc
     2161 ggcagcaacg cagctcccgt cgaccgctga atcagaagtt gcacgggcac tgctcggcgg
     2221 tggtggtaga gtgctacgtg catggtcgct ccttcgcgcc actacacgtt cgaggactac
     2281 ctgagcgtcg aggagatgag cgccgtccgt catgaattcc tcaacggcga gatcttcgcg
     2341 atggcaggcg ggacgccgga gcatgcagcc ctgtcggcag ccgccgtgct cgttctcggg
     2401 cgtcggctcg aaggccagcc gtgccggccc tacagcgccg atctgcggat ccgtgtggtc
     2461 gcgacgggcc tcgcgaccta cgcggacacg gcggtgatct gtggggatcc gatccgtgat
     2521 ccggcgtcgc cgacccacgt gacgaacccg atcgtgatcg tcgaggtgct cagcaagagc
     2581 acggaggcgt acgatcgagg agagaagcgc gagcactacc agcagatcgc atcgctccgc
     2641 gagtacgtgc tggtcgcgca ggacaaacgt gagatcgagg tctacgcgcg tcccacaggt
     2701 ggcatctggc agcgttccgt ctaccgagcc gcggagaccg tggacctccc gtcgatcggg
     2761 atccgcttca ccgtcgacga gctgtacagc gctgcgggcg taccgtagcg gccgtccagg
     2821 caacatagtt ccggacggaa cctgaacaaa gaattccgcg gccgcgcggg gtaccggcta
     2881 gccggtcgga gcgcgatcgc ctatgttggt tccgacgacg acgtcactgt tcccggagga
     2941 accgatggat ggccccgtgg tcccagtctc acacgtccgg gcctccggac agcgcgacaa
     3001 gctgcggctg gcgctcacgg tcaacgacga ggatcgcgat ctggtcgtcg cgtcgtacaa
     3061 gacgctgctc gaggtgctgc gtgaggatct cgcactgacc gggaccaagc acggctgcga
     3121 gctcg
//
LOCUS       DASVOR010000470         5565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_208890,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000470 DASVOR010000000
VERSION     DASVOR010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5565
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_208890"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1774)
                     /locus_tag="VF516_09350"
     CDS             complement(179..1774)
                     /locus_tag="VF516_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107920.1"
                     /translation="MSVTTILGAAAALLCGALSGCSFILDSSTKQCSVDTDCDSFGNH
                     PKCMSNVCVDSGLGPANCFFPTADKPLSKQADFLNQCTTSTHQEFNNCDRLTFGCNGG
                     PTTLPPQTTPPMTMPQGSNTAPTPPSNLCTDNAPTSGGTPNMIWLFGSSDFGPLMRAA
                     QPSLSAAATPYRAVFQNASSCAGVTAIFSNLSGATDPTKRLMKDPADPAKGGWAFYFD
                     DNGNQVNCRIDPMGTSVGVPITIGISDLYSTTCGFTPTSTTVTEYTGPVVPFVFATKA
                     ASKEVSISAEAAHMVFANGGMPPSGSGMKPAMPWIDYTKYYIRNTTAGSTVLSALLLN
                     VKISTTNPFWGIDRVSTDNLRDALLAATDPDAAIGILSIDFYDKNRGNLKGLYLQSFN
                     QSAGYLPDSKPTTTDKMNVRDGHYPLWGYVHFVVPLDSIGGTSQAANAMTLLFSVDKL
                     DQRLVDAIIAASEVPQCAMKVQRRGELNSADGSGDFKVRTGFSCGCYFDAKTTNHSDC
                     KTCSTSDECPNGGSCNYGYCEAN"
     gene            2061..2861
                     /locus_tag="VF516_09355"
     CDS             2061..2861
                     /locus_tag="VF516_09355"
                     /inference="COORDINATES: protein motif:HMM:NF018473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HEX8107921.1"
                     /translation="MRALLFCGLGMGLCLGLPTACARADGEPKPPAAERGAARSARAP
                     AGAEGPLVLELFTSQGCSSCPPADELLAKLARTGSLGDRALAPLSFHVDYWNDLGWAD
                     PYSSPAWTERQHQYARVLDDRVYTPELVVGGAAGMVGSNLAAVTDAIQRAARPALLAA
                     TARWTADAVTVTTAAPGDAEVWVAIWEDGTRTHVARGENAGETLMSERVVRHLERAAA
                     AGQNASLAIRLDPAWHASGAVVFAQRADRRIVASALLGREPPARTRGP"
     gene            complement(2888..3874)
                     /locus_tag="VF516_09360"
     CDS             complement(2888..3874)
                     /locus_tag="VF516_09360"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HEX8107922.1"
                     /translation="MRNAIERDVTAGCTPPTDGDPSRAGALSAVAAVVRPVVRSAIGL
                     LADLLDRIVHGIRCLAPLALGFGCQANSETPDVAPVIEPVPASTVARTEDTGPRPLGQ
                     FTITFYYVIGEDEVGPPRVANENRNENRNGSRSESREEEAETELAAITPDLVTLYGGG
                     GRCEPIADVSKEFASQLAVQGTGKLHDGRVLNIWGPCNCKRSPCFKVTQAQWGTAGSG
                     KPLQPFRTVAVDPRVVKLGSWLYVPLLEGRTMPGRGPWGGYVHDGCVIADDTGGHILG
                     NRLDLFVGRKGYFLGLSGSGGSHAWARHVPVFDGTGICDRKDRRVARKAASI"
     gene            complement(4021..>5565)
                     /locus_tag="VF516_09365"
     CDS             complement(4021..>5565)
                     /locus_tag="VF516_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107923.1"
                     /translation="ERAAREAAERDAAASEDAGEVRGASGFMDTRLAFTLTNENMLVR
                     PGETVPSVPGWRFGTPSSLGVLFFDGYDTRYSGFETLSHAVMYRDYRSGHLQAEGALV
                     VRIDDLSETRLGLSDDGSYVTVSDWKDPGHKDPTRISLTVFPVSSDRFRLGYSYRLSW
                     GGSPEYTGAASSAPGSAPGGSVPGIKLQLDTPRTYAFAGAKSATLIDPKTGQARSALA
                     VLGGAGVDPFPMFRLEANGGYFDRGYNELPGVQREKVRLFGGSVQASIHRGMPLRSSL
                     DYQLYKFHGEAISDLFAPERYPGGLAWLAQTEFTMLGQTLADPDAPGRTTTQYGQAGD
                     LNVRVKLDRLRLRLDLSYRDLAFLLHTRPSLPVDEQFPAGYTATADYFGAIGLDRNWD
                     DWLTLGLVAGVEKPATLTSPQGIPGVAPDTMGPMTAVIRSNNVDTQITVLSPGASAAR
                     QYAAKAMAKVDFARIFSMLVELFYSYDSTGRVAPPCSQPQPCTLSFVAGSAHQLGLNA
                     TLQARF"
BASE COUNT          835 a         1934 c         1921 g          875 t
ORIGIN      
        1 gccgcgcccg gtcgtggcct gcgcgccggc cccgcactcg ccggtgccga ccatcgcgcc
       61 aggcggatcc ggcggatcgg tcggatcacc cggctccggc gcctcgctcg cggcgatcgt
      121 ccgcgaggag atcgtgcgcg cgctcggcga gccgggggcg tgaccgccgg cggctaccct
      181 agttggcttc gcagtagccg tagttgcagg agcccccgtt cgggcattcg tcggaggtac
      241 tgcaggtctt gcagtcggaa tggttcgtgg tcttggcgtc gaagtagcag ccgcacgaga
      301 accccgtccg caccttgaag tcgccgctgc cgtccgctga gttgagctcg ccacgccgct
      361 gcaccttcat cgcgcactgc ggcacctccg aggcggcgat gatcgcgtcg accaggcgct
      421 gatcgagctt gtcgacgctg aacagcaagg tcatggcatt cgcggcctgg ctggtgccgc
      481 cgatgctgtc gagcggtacg acgaagtgca cgtagcccca cagcgggtag tggccatcgc
      541 ggacgttcat cttgtcggtc gtcgtcggct tcgagtccgg caggtacccc gccgactgat
      601 tgaacgactg caggtacagc cccttcaggt tgccgcggtt cttgtcgtag aagtcgatcg
      661 acaggatgcc gatcgccgca tctggatcgg tcgccgccag cagcgcgtcg cgcaggttgt
      721 cggtcgacac ccgatcgatg ccccagaacg ggttggtcgt ggagatcttc acgttgagca
      781 gcagcgcgct cagcacggtc gagccggcgg tggtgttgcg gatgtagtac ttggtgtagt
      841 cgatccacgg catggcgggc ttcatgccgc tgcccgacgg gggcatgccg ccattggcga
      901 acaccatgtg cgccgcctcg gcgctgatcg acacctcctt cgacgccgcc ttggtcgcga
      961 acacgaacgg gaccaccggg ccggtgtact cggtcaccgt cgtggacgtc ggtgtgaacc
     1021 cgcaggtcgt ggagtagaga tcggagatgc cgatcgtgat cgggacaccg accgacgtcc
     1081 ccatcgggtc gatccggcag ttgacctggt tgccgttgtc gtcgaagtag aatgcccagc
     1141 cgcccttggc cgggtccgcc gggtccttca tcagccgctt cgtggggtca gtcgcgcccg
     1201 acaggttcga gaagatcgcc gtgacgcccg cgcatgacga cgcgttctgg aacaccgcgc
     1261 ggtagggcgt ggcggcagcg gacagcgacg gctgcgcggc gcgcatcagc ggcccgaagt
     1321 ccgacgagcc gaacagccag atcatgttcg gcgtgccgcc cgaggtcggc gcattgtcgg
     1381 tgcacaggtt gctcggaggc gttggcgcgg tgttcgagcc ttgcggcatc gtcattggcg
     1441 gtgtcgtctg cggcggcagc gtcgtggggc caccgttgca tccgaaggtg aggcggtcgc
     1501 agttgttgaa ctcctggtgc gtcgacgtcg tgcactgatt gaggaagtcg gcctgcttgc
     1561 tcagcggctt gtccgccgtc gggaagaagc agttcgccgg gccgagcccc gaatcgacgc
     1621 acacgttgct catgcacttc ggatgattgc cgaagctatc gcagtcggtg tcgacggagc
     1681 attgcttggt cgaggagtcg aggatgaagc tgcagccgct gagcgctccg cacagcagcg
     1741 ccgcagccgc accgaggatc gtggtgacgg acattctttc accccaaagg tgttgtaggc
     1801 accccatggc cgaaacggta cctaatcgaa aaccgacggt acagctggat ttcggaaact
     1861 aaccgagcgc gcgacgaacc gcacgaaaac cgaggtgctg gcgcagcacg cgccgtaagc
     1921 agcgtcacgc ggctgaaaca tgacgacggg ttggtgtatc agctgacacc aagtgcaccg
     1981 agcgttagac ggtgcttggc gcaggtcgtc atacagcaac gggagcagta acctcggtgc
     2041 ctcacggacg taccgacgcc atgcgcgccc tcctcttctg cggcctcggc atgggtctct
     2101 gcctcggcct gcccaccgcc tgcgcccgcg ccgacggcga gccaaagccc cccgccgcgg
     2161 agcggggtgc agcgaggtca gcgcgcgcgc cggccggcgc cgagggtccc ctcgtgctcg
     2221 agctgttcac ctcgcagggc tgcagcagct gcccgcccgc ggacgagctg ctcgccaagc
     2281 tcgcgcgcac cggcagcctc ggcgaccgcg cgctcgcacc gctgtcgttc cacgtcgact
     2341 actggaacga cctcggctgg gccgatccgt actcctcgcc ggcgtggacc gagcgacagc
     2401 accagtacgc ccgggtgctc gacgaccgcg tctacactcc cgagctggtg gtcggcggcg
     2461 ccgccggcat ggtcggctcg aacctcgcgg ccgtgaccga cgcgatccag cgcgccgccc
     2521 gcccggccct gctcgccgcg accgcccgct ggaccgcaga cgccgtcacc gtgaccaccg
     2581 cagctcccgg cgatgccgag gtctgggtgg cgatctggga ggatggcacc cgcacccatg
     2641 tggcgcgcgg cgagaacgcc ggcgagaccc tgatgagcga gcgcgtcgtc cggcacctgg
     2701 agcgcgccgc ggccgcgggg caaaatgcct ctctcgcgat ccgcctcgat cccgcctggc
     2761 acgcctccgg cgccgtggtg ttcgcgcagc gcgcggatcg ccgcatcgtg gcatccgccc
     2821 tgctaggtcg cgagccgccg gctcgcaccc gcgggccttg agcttcggat cggccggctc
     2881 ggccgactca gatcgatgct gccttgcggg cgacccgccg gtccttgcgg tcgcagatcc
     2941 cggtgccatc gaacactggc acgtgacgtg cccaggcgtg gctgccgccg ctgccggaca
     3001 ggccgaggaa gtaccccttg cggcccacga acaggtcgag ccggttgccc aggatatgac
     3061 cgcccgtgtc atcggcgatc acgcagccat cgtgcacgta gcctccccac ggtcctcgac
     3121 ccggcatcgt gcggccttcg agcagcggca cgtagagcca ggagccgagc ttcaccaccc
     3181 ggggatcgac cgccacggtg cggaacggct gcagcggctt gccgctgccc gccgtgcccc
     3241 attgcgcctg ggtgaccttg aagcacgggc tgcgcttgca gttacagggg ccccagatgt
     3301 tgagcacgcg gccgtcgtgc agcttgccgg tgccctgcac cgcgagctgc gaggcgaact
     3361 ccttggaaac gtccgcgatc ggctcgcacc gaccgccgcc cccgtacagc gtgaccaggt
     3421 ccggcgtgat cgccgcgagc tcggtctcgg cctcctcttc gcggctctcg ctccggctcc
     3481 cgttccggtt ctcgttccgg ttctcgttgg ccacccgcgg gggcccgacc tcgtcctccc
     3541 cgatcacata gtagaaggtg atggtgaact gcccgagcgg gcgtggcccg gtgtcctcgg
     3601 tgcgagccac cgtggacgcc ggcaccggct cgatcaccgg agccacgtcg ggggtctcgc
     3661 tgttcgcctg gcagccgaag ccgagcgcga gcggcgccag acaccggatt ccatggacga
     3721 ttcgatcgag cagatcggcg agcaggccga tcgccgagcg cacgaccggc cgcacgaccg
     3781 cggcgacggc gctcagcgcg ccggcgcgcg atggatcgcc gtccgtgggc ggtgtgcaac
     3841 cagcggtcac gtcgcgttcg atcgcgttcc tcatacatgc ccgagccgac gtttccgctg
     3901 gggctaaagg agtctagcct cgatccagcg agcgcaacgg cgacgcgccg ggaaaactca
     3961 ctgtcatctg actgccatga tgagcgctat tccgatggcc ggaccgccgc gaagacgcgc
     4021 tcagaaccgc gcttgcaggg tcgcgttcag gccgagctgg tgcgcggagc cggccacgaa
     4081 cgacagggtg cacggctgcg gctggctgca cggcggcgcc acgcgccccg tgctgtcgta
     4141 gctgtagaac agctcgacca gcatgctgaa aatcctcgcg aaatcgacct tggccatcgc
     4201 cttggccgcg tactggcgcg ccgcgctcgc cccgggggac agcacggtga tctgggtatc
     4261 gacgttgttg ctgcggatga ccgcggtcat cggccccatg gtgtcgggag cgacgccggg
     4321 aatcccctga ggggacgtca aggtcgccgg cttctccaca ccggcgacca ggccgagcgt
     4381 cagccagtcg tcccagttgc ggtccagccc gatcgcgccg aagtagtccg ccgtcgcggt
     4441 gtagccggcc gggaactgct catcgaccgg caagctcggc cgcgtgtgca gcaggaaggc
     4501 gagatcgcgg tagctcagat cgagccgcag ccggagccga tcgagcttca cccggacgtt
     4561 gaggtcgccg gcctggccgt actgcgtggt cgtccggccc ggggcgtcgg gatctgccag
     4621 ggtctgcccg agcatggtga actcggtttg cgccagccag gccaggccgc cggggtatcg
     4681 ctccggcgcg aacaggtcgc tgatcgcctc gccgtgaaac ttgtagagct ggtagtcgag
     4741 cgacgatcgc agcggcatgc cgcggtggat cgatgcctgc acggacccgc cgaacagccg
     4801 gaccttctcg cgctggacgc ccggcagctc gttgtagccg cgatcgaagt agccgccgtt
     4861 ggcctccagc cggaacatcg ggaacggatc gacgccggcg ccgccgagca ccgccagcgc
     4921 ggaccgcgcc tggccggtct tcggatcgat cagcgtggcg ctcttggccc ccgcgaaggc
     4981 atacgtgcgg ggggtgtcga gctgcagctt gatccccggg accgaccccc ccggggccga
     5041 ccccggggcc gatgacgcgg cgcccgtgta ctcggggctg ccaccccagg acagccggta
     5101 ggagtagccg agccggaagc ggtcggatga caccgggaac acggtcaggc tgatccgggt
     5161 cggatccttg tggccgggat ccttccagtc gctgaccgtg acgtacgagc cgtcgtccga
     5221 caggccgagg cgggtctcgc tcaggtcgtc gatccggacc acgagcgcgc cctcggcctg
     5281 gagatgaccg ctccggtagt cgcggtacat gaccgcgtgc gacagggtct cgaaccccga
     5341 gtaccgggtg tcgtagccat cgaagaacag cacgccgagc gagctcgggg tgccgaaccg
     5401 ccagcccggc acgctcggca ccgtctcgcc cggccgcacc agcatgttct cgttggtcag
     5461 cgtgaacgcg agccgggtgt ccatgaaccc gctcgcaccc cggacctcgc cggcgtcctc
     5521 gctcgccgcg gcgtcgcgct cggcggcctc ccgggcggcg cgctc
//
LOCUS       DASVOR010000471         5571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_92329,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000471 DASVOR010000000
VERSION     DASVOR010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5571
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92329"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..802)
                     /gene="thpR"
                     /locus_tag="VF516_09370"
     CDS             complement(89..802)
                     /gene="thpR"
                     /locus_tag="VF516_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEX8107924.1"
                     /translation="MKPGKRLFVGVRISVAASNALAGCAETLARRARDAGADLRWVAP
                     INYHVTLAFLGWTGVEAIDRVCDALAAAVAGESRTTFRTTRLGAFPSLDHASVLWAGI
                     EDGAALIRLARSTGAAMAGLGFAPRGIERDPRPFHPHVTIARLRTPRAIREIVLPMAE
                     QMFGDTRIDAVTLFESETKLNGSVYREVQKILFERAVRTPPEPSKRQTDALERGTPDR
                     EPDDSLTDDGWPRGQGPTE"
     gene            complement(799..2115)
                     /locus_tag="VF516_09375"
     CDS             complement(799..2115)
                     /locus_tag="VF516_09375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HEX8107925.1"
                     /translation="MRAEILTIGDELCRGEIVDTNSSHLAARLWDLAITARWMTSCTD
                     DAADIASALAQAVARADLVVCSGGLGPTEDDLTVDVVAGLLGVPAVIDPPSRARLETW
                     FAQRVLPSVRATGDAMPAAATVSDVLLRQVRVPAGARVHLNPAGLAPGFEVVLRGVPV
                     LFLPGFPREIQAILETGMERRFAELREARGEVERIARRIYRVFGRGESQISQACRGMI
                     DGVPGASIHYQVKFPETLVKLVVRDRDAGAADRALAALDTEIRARLRGYLYGDGDETL
                     IDRTVRRLIEAGKTVATAESCTGGMIGELLTRRPGSSRAFLGGAIVYSNAEKVRQLGV
                     REATLEGHGAVSEPTVREMALGARERFGADLAVAVSGIAGPDGGTPDKPVGTVWLALA
                     GAGTEAGNGADGADHVTTKLLAWPGARDQIRTLASWWALRLIDEAL"
     gene            2237..3004
                     /locus_tag="VF516_09380"
     CDS             2237..3004
                     /locus_tag="VF516_09380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX8107926.1"
                     /translation="MKIRYAAKTDVGMKRTHNEDYFSLIEDEQLFLVADGMGGHASGE
                     VASKMAAETIGEFYQRTREDEDATWPYKMDRSLSYIENRLVCGIKLANLRIFETSNRD
                     LRYKGMGTTIVSVLVSGDKIYVGHVGDSRVYRVREGGIVQLTRDHSLLEDYKEAKPDM
                     TEEEERNFPHKNVITRALGMRETVQVDIRSHQIKSGDVYILCSDGLSGMVVDTQICQI
                     ATNAKSLERAVAELVDAANRNGGTDNVTTLLLQCLAA"
     gene            3018..3611
                     /locus_tag="VF516_09385"
     CDS             3018..3611
                     /locus_tag="VF516_09385"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8107927.1"
                     /translation="MRYRVTSGTLTVRARSRIHDTTTVWNRITGEIAADPDTLAMAPV
                     RAPGPDPGPGGPGGPGGPGGPDGPDDGGLAGTFARFEVDMTAFDAGDFLKNRKLRKDF
                     TLEAHPRASFVLTGLQGVVRTGTAFTATAVGTLEWRGKRVGLTLSGRGTLDAVAIEAS
                     ATFELDIRWHGLAAPKFLIFKIEDEVTVDVAIRGVAA"
     gene            3608..4090
                     /locus_tag="VF516_09390"
     CDS             3608..4090
                     /locus_tag="VF516_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107928.1"
                     /translation="MTARGAAALVAVALAACTSRAPEHDGIGNWRFPHTRLQNIQEGR
                     CQPTDLSDGRKATWCFAQPPIRIGQRSAEVDLYFLGGEPSAPLIEIQLKVRGCHEDEL
                     EQWLRAGFGLPMETRPQRAYWKNSFLWIGALMPSDPGRCLVHVLPLSENAEIDRIKQQ
                     "
     gene            complement(4406..4909)
                     /locus_tag="VF516_09395"
     CDS             complement(4406..4909)
                     /locus_tag="VF516_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEX8107929.1"
                     /translation="MSERSEFRPDDTGELKERWRAYVQDRRLNVTAQREAIVEQFLRT
                     REHVSIDELLLKVRKRQPKVGYATVYRTLKLLVDSGLAVERQFGDGQARYEVVGDHHD
                     HLICVKCGLILEFEDDEIERLQDKIAQRLGGFTVLRHRHELYGLCPKAAGEAGGACPR
                     EQSKRGS"
     gene            5149..5403
                     /gene="xseB"
                     /locus_tag="VF516_09400"
     CDS             5149..5403
                     /gene="xseB"
                     /locus_tag="VF516_09400"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEX8107930.1"
                     /translation="MGFDQILARLREVVIRLESGELSLEQSLVIYEEGVQLARRGQQL
                     LASAEKRVEILISASGGIEVAPFVDPGSLGSGGPAGSSVE"
BASE COUNT          838 a         1976 c         1952 g          805 t
ORIGIN      
        1 gtcggccttg ccggctgaat cgccccgccc ggcgccgggg ggcggcgcgc ccggttgtgt
       61 ctctttggtg gccatctctg acgtgctcct actcggtggg gccctgaccc cgcggccagc
      121 cgtcgtcggt cagcgaatcg tctggctcgc ggtcgggtgt gccccgttct agagcgtccg
      181 tctgacgttt ggacggctcg ggaggggtcc gaacggcccg ttcaaacaga attttttgca
      241 cctcgcgata gacggaacca ttcaatttcg tttcgctttc gaacaacgtc accgcatcga
      301 tccgggtgtc accgaacatc tgttcagcca tcggtaacac gatctcgcga atcgcgcgag
      361 gggtccgcag ccgcgcgatg gtcacgtggg ggtggaatgg ccggggatct cgctcgatcc
      421 cacggggggc gaagccgagc cccgccatcg cggcgccggt cgaccgcgcg agccggatga
      481 gcgccgcgcc atcctcgatc ccggcccaca gcacgctcgc gtggtccagc gaggggaacg
      541 ccccgagccg cgtggtccgg aaggtcgtgc ggctctcgcc ggcgacggcc gctgccagag
      601 cgtcgcacac ccggtcgatc gcctccaccc cggtccagcc gaggaacgcg agcgtcacgt
      661 gatagttgat cggcgcgacc caccggaggt cggccccggc gtcccgggcg cgccgggcga
      721 gcgtctcggc gcagcctgcg agcgcgttcg aggccgccac cgagatccgc accccgacga
      781 acagccgctt gcccggcttc atagcgcctc gtcgatgagc cggagcgccc accagctcgc
      841 cagcgtgcgg atctggtcgc gggcccccgg ccacgccagc agcttggtcg tcacatggtc
      901 tgccccgtcc gccccgttcc cggcctccgt gcctgcgcct gccagcgcga gccacaccgt
      961 gccgaccggc ttgtccggcg tcccgccgtc ggggccggcg atgcccgaca ccgcgaccgc
     1021 gaggtcggcg ccgaaccgct cgcgcgcccc gagcgccatc tcgcgcacgg tcggctcgct
     1081 gaccgcgccg tggccctcca gggtggcctc gcggaccccg agctggcgga ccttctcggc
     1141 gttggagtag acgatcgcgc cgccgaggaa cgcccgggag ctgcccggcc ggcgggtcaa
     1201 cagctcgccg atcatgccgc cggtgcacga ctcggcggtc gcgacggtct tgccggcctc
     1261 gatcagccgg cgcaccgtgc gatcgatcag ggtctcgtcg ccgtcgccgt acaggtagcc
     1321 gcgcagccgc gcgcggatct cggtgtcgag cgcggccagc gcgcggtcgg cggcgccggc
     1381 gtcgcggtcg cggaccacca gcttgaccag ggtctcgggg aacttcacct ggtagtggat
     1441 cgacgcgccg gggacgccgt cgatcatccc gcggcaggcc tgcgagatct gcgactcgcc
     1501 gcgaccgaac acccggtaga tccggcgggc gatccgctcg acctcgccgc gggcctcgcg
     1561 cagctcggcg aaccggcgct ccatcccggt ctccaggatc gcctggatct cgcgcgggaa
     1621 ccccgggagg aacagcaccg ggaccccccg gagcacgacc tcgaagccgg gtgcgaggcc
     1681 ggccgggttg aggtggacgc gggcgcccgc gggcacccgg acctggcgga gcaggacatc
     1741 gctgaccgtg gccgcggccg gcatggcgtc gcccgtcgcc cggacggacg gcagcacgcg
     1801 ctgcgcgaac caggtctcga ggcgggcccg cgacggcggg tcgatcaccg ccgggacccc
     1861 gagcagcccg gcgaccacgt cgaccgtcag atcgtcctcg gtcggaccca ggccgccgga
     1921 gcacacgacc agatccgccc gggccaccgc ctgcgcgagc gcgctggcaa tatccgccgc
     1981 gtcatcggta cagctcgtca tccaccgggc ggtgatcgcc aggtcccaca gccgtgcggc
     2041 caggtgagac gagttcgtgt cgacgatctc gccacggcac agctcgtccc cgatcgtcaa
     2101 gatctcggcg cgcatccgag ggggaccata gcgcgaccgg cccgccccgc gggatacaac
     2161 gccacgcggt tgacgcgctc gggacgcgtt gcctatcatt tctgtacgcg gaggcgtgta
     2221 gctccgcagg aatctgatga agatccgcta cgccgccaag accgacgtgg gcatgaagcg
     2281 gacccacaac gaggactatt tctcgctgat cgaagacgag cagctgttcc tggtcgccga
     2341 cggcatgggc ggccacgcct cgggcgaggt cgcctcgaag atggccgccg agacgatcgg
     2401 cgagttctac cagcggacgc gcgaggacga ggacgcgacc tggccgtaca agatggaccg
     2461 ctcgctgtcc tatatcgaga accgcctggt gtgcggcatc aagctggcca acctgcggat
     2521 cttcgagacc tcgaaccgcg acctgcgcta caagggcatg ggcacgacga tcgtctcggt
     2581 cctggtgtcc ggcgacaaga tctacgtcgg ccacgtcggc gactcgcggg tgtaccgggt
     2641 gcgcgagggc ggcatcgtgc agctgacccg cgaccactcg ctgctcgagg actacaagga
     2701 agccaagccc gacatgaccg aggaggaaga gcgcaacttc ccccacaaga acgtgatcac
     2761 gcgcgcgctc ggcatgcgcg agaccgtcca ggtcgacatc cgcagccacc agatcaagag
     2821 cggcgacgtg tacatcctgt gctcggacgg cctgtcgggc atggtcgtcg acacccagat
     2881 ctgccagatc gcgaccaacg ccaagagcct ggagcgcgcg gtcgccgagc tggtcgacgc
     2941 ggccaaccgc aacggcggca ccgacaacgt cacgacgctg ctgttgcagt gcctcgcggc
     3001 gtagaccagg gttgggggtg cgataccgcg tcacctccgg cacgctgacc gtccgcgcgc
     3061 gatcgcggat ccacgacacc accaccgtgt ggaaccggat cacgggcgag atcgccgccg
     3121 atccggacac cctggccatg gccccggtcc gagccccggg gccggacccg ggtccgggcg
     3181 gtccgggcgg tccgggcggt ccgggcggtc cggacggtcc ggacgatggt ggcctcgccg
     3241 ggacgttcgc gcgcttcgag gtcgacatga cggcgttcga cgccggcgac ttcctcaaga
     3301 accgcaagct gcgcaaggac ttcaccctcg aggcccaccc gcgggcgagc ttcgtgctga
     3361 ccgggctgca gggcgtggtg cggaccggga cggcgttcac cgcgaccgcg gtcggcacgc
     3421 tggagtggcg cggcaagcgc gtcgggctca cgctgtccgg gcgcggtacg ctcgacgccg
     3481 tggcgatcga ggccagcgcg accttcgagc tcgatatccg ctggcacggg ctggccgcgc
     3541 cgaagttcct gatcttcaag atcgaagacg aggtcaccgt cgacgtcgcg atccgggggg
     3601 tggcggcatg accgcgcgcg gcgccgcggc gctggtcgcg gtcgcgctcg cggcgtgcac
     3661 cagccgcgcg cccgagcacg acggcatcgg caactggcgg ttcccgcaca cccggctcca
     3721 gaacatccag gaggggcggt gccagccgac cgatctgagc gacgggcgca aggcgacctg
     3781 gtgcttcgcg cagccgccga tccggatcgg ccagaggagc gccgaggtcg acctctactt
     3841 cctgggcggc gagccgagcg ccccgctgat cgagatccag ctcaaggtcc gcggctgcca
     3901 cgaggacgag ctcgagcagt ggctgcgcgc cgggttcggc ctcccgatgg agaccagacc
     3961 gcagcgcgcc tactggaaga actcgttcct gtggatcggc gcgctgatgc cgagcgaccc
     4021 cggccgctgc ctggtccacg tcctgccgct gtcggagaac gcggagatcg accggatcaa
     4081 gcagcagtag cgaggacgga agccgggtcc gaaacctcga ccttgtcctc gacgatgacc
     4141 ttgacccgaa cgttgaggtt gacggcgacg gacggcgacg gtgacgtcga ccccaccttt
     4201 gaccttgcac cttgaggtga tcgcggagct tcccgcgaag agccacgcgg agatcgacga
     4261 cgatgagccg aggtcgacgt cgagatacgt cgaggtcgag ggtggggtcg acgtcgtacg
     4321 tcgccctcga cctcaacgtt cggggtcaac gtcgacgtca aaccgtcaac gtcaccaccg
     4381 cttgccccgc atcgcccctt ccccgtcagc tgccgcgctt gctctgctca cgcggacacg
     4441 cgccgccggc ctcgcccgcg gccttggggc acaggccgta cagctcgtgg cggtggcgga
     4501 gcacggtgaa gccgccgagc cgctgcgcga tcttgtcctg caggcgctcg atctcgtcgt
     4561 cctcgaactc gaggatcagg ccgcacttca cgcagatcag gtgatcgtgg tggtcgccga
     4621 cgacctcgta gcgcgcctgg ccatcgccga actggcgctc gacggccagg ccgctgtcga
     4681 ccagcagctt gagcgtccgg tagaccgtgg cgtagccgac cttgggctgg cgcttgcgca
     4741 ccttgagcag cagctcgtcg atcgagacgt gctcgcgggt gcggaggaac tgctcgacga
     4801 tcgcctcgcg ctgcgcggtg acgttgagcc ggcggtcctg gacgtaggcg cgccagcgct
     4861 ccttgagctc gcccgtatcg tcgggacgga attccgagcg ctcgctcatg gcgccagctt
     4921 ccctgcaagc gcgcgggtcg tcaagcactg ttcacgtgct ccggcgctcg cgaaatctga
     4981 ttcgtgtcgg gatcgttcta cggtgagccc gcgaggtaag cggagtgact ttcggcgacg
     5041 cgttcgctat ggtgcgatgc gatggctgac gcgaccacct ccggcggtgt cgccaacggc
     5101 tcggccggga ccggaggctc tggctccggt cccggggagc ccggagcgct ggggttcgac
     5161 cagatcctcg cgcggttgcg tgaggtcgtg atccggctgg agtccggcga gctgtcgctg
     5221 gagcagtcgc tcgtgatcta cgaagagggc gtacagctcg cgcggcgcgg ccagcagctg
     5281 ctcgcgagcg cggagaagcg ggtcgagatc ctgatcagcg cgtccggcgg catcgaggtc
     5341 gcgccgttcg tcgatcccgg gtcattaggt tcggggggtc cagcggggtc gagcgtcgag
     5401 taggctccgg tcgtcatgac cccgcaagcc accctcgcgc cggcgagcgc gccgcccgac
     5461 ttgcaccgct tcctcgacga ggtccgccgg ttgatcgacg ccgagctcga tcgccggctc
     5521 gcgccgccgg ccgacgaccc cggacggctg atcgccgcga tgcactacgc g
//
LOCUS       DASVOR010000472         2726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_121225,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000472 DASVOR010000000
VERSION     DASVOR010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121225"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1133
                     /locus_tag="VF516_09405"
     CDS             150..1133
                     /locus_tag="VF516_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8107931.1"
                     /translation="MTANATARVTAAGGSALGAGDAVLELVGLGKDYGARTAVHAIDL
                     TLSRGEIFGLLGPNGAGKTTTISMACGVVPPSRGTARVAGADIRTRTFDAKRAIGLVP
                     QDLALYDELSPRQNLAFFGQLYGLAGGELARRIDWALGIAGLTDRACDLVKTFSGGMK
                     RRLNLAAGLIPEPTLLVLDEPTVGVDPQSRRHIFEAIRALRGRGMTILYTSHYMEEVE
                     ELCDRVAIMDAGRIVASGRIDELTAQHTAGGLEIELDGDEAVIAAAAAASAPLGASRA
                     GTMLHLEARPPYAPAIAAIEATGAQIKRIGARRADLETVFLALTGRGLRDA"
     gene            1133..2224
                     /locus_tag="VF516_09410"
     CDS             1133..2224
                     /locus_tag="VF516_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8107932.1"
                     /translation="MLIPTLRKELLLLRRDPRALVRLIVLPLLFIGIFGYLFKNAGEK
                     QREVRPIAVWNGAGPAGEVLTGGLAGSPLFTIVPAASADAVEAAVAHHDPVVGLVIPP
                     DYDLAHGRRAILLIDAAESPLTAGPLRAAVIDALTRAVTRSAPSQIVDVRSPAGAARS
                     LDDRTSSFQLAVPGNAVLFGFFIALTCALSFAEERRSGTWRRLLASPVPAWKLLATKL
                     VPYVALGTIQVGLLFAIGALGFGMQIGGSIAALAVLTVGVAACATTLGLLIAALSSTE
                     KQISSLGSVLIMIMGLVGGCMFPRALMPHALQQVGLFVPHGWALEGYYTLLIGHGTGF
                     ADVLRPLGAVYGFVLLFAVAGLRRFRFDQ"
     gene            2314..>2726
                     /locus_tag="VF516_09415"
     CDS             2314..>2726
                     /locus_tag="VF516_09415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107933.1"
                     /translation="MSAAALPHGGHRWVRVRLWSTPEPRAHVEVRAVRWRNGSHDIET
                     AELTRGEAELAEASRSELVALLAAPGAAALAQVESTVRDGLRCRVTVLDRAADRVQEG
                     SCNLAGVPDAARDHPSVRLVQAVLRAGAAVPVPEL"
BASE COUNT          347 a         1078 c          914 g          387 t
ORIGIN      
        1 agcccgccga tcacctgggc gtggcgctcg ccggtcgact cgaagaacac cgtgtccatg
       61 aatggcaaat ccgggcccga gcctagcaag ccttgcggcg cacgtgccga cacagcccgg
      121 gcatgatagg gctgctacca gggggaatca tgacggccaa cgcaacggcc cgcgtgacgg
      181 cagccggcgg cagtgccctc ggggccggcg acgcggtgct ggagctcgtc ggactgggca
      241 aggactacgg cgcgcgcacc gcggtccacg cgatcgatct gacgctgtcg cgcggcgaga
      301 tcttcggcct gctggggccc aacggcgccg gcaagaccac aacgatctcg atggcctgcg
      361 gggtcgtgcc gccgagccgc ggcaccgcgc gcgtcgccgg ggccgacatc cgcacccgga
      421 cgttcgacgc caagcgcgcg atcggcctcg tgccgcagga cctcgcgctc tacgacgagc
      481 tgtcgccgcg ccagaacctg gcgttcttcg gccagctcta cggcctggcc ggcggcgagc
      541 tcgcccgccg gatcgactgg gcgctcggca tcgccgggct caccgaccgc gcctgcgatc
      601 tggtcaagac gttctccggc ggcatgaagc gcaggctcaa cctcgccgcc ggcctgatcc
      661 ccgagccgac cctgctggtg ctcgacgagc ccacggtcgg cgtcgacccg cagagccggc
      721 gccacatctt cgaggcgatc cgcgcgctgc gcggccgcgg catgaccatc ctctacacca
      781 gccactacat ggaggaggtc gaggagctgt gcgaccgcgt cgcgatcatg gacgccggcc
      841 ggatcgtcgc cagcggccgg atcgacgagc tgaccgcgca gcacaccgcc ggcggcctcg
      901 agatcgagct cgatggcgac gaggccgtga tcgccgccgc cgccgccgcc tcggcgccgc
      961 tcggcgccag ccgcgccggc accatgctcc acctcgaggc ccgcccgccg tacgcccccg
     1021 cgatcgccgc gatcgaggcc accggcgcgc agatcaagcg gatcggcgcc cgccgcgccg
     1081 acctcgagac cgtgttcctc gcgctcaccg ggcgcggatt gcgagacgcc tgatgctgat
     1141 ccccaccctc cgcaaggagc tgctcctgct ccgccgcgat ccgcgcgcgc tggtccgcct
     1201 gatcgtcctg ccgctgctgt tcatcgggat cttcggctac ctgttcaaga acgcgggcga
     1261 gaagcagcgc gaggtccggc cgatcgcggt gtggaacggg gccggccccg ccggcgaggt
     1321 gctgaccggc ggactcgccg gctccccgct gttcaccatc gtcccggccg cgagcgcgga
     1381 cgccgtcgag gcggcggtcg cccaccacga tccggtggtc ggcctcgtca tcccgcccga
     1441 ctacgacctg gcgcacggtc gccgcgccat cctgctgatc gacgcggccg agtcacccct
     1501 gaccgccggc cccttgcgcg ccgccgtcat cgacgccctg acccgcgccg tgacccgcag
     1561 cgcgccgtcc cagatcgtcg acgtccgttc cccggccggc gccgcccgct cgctcgacga
     1621 ccgcaccagc tcgttccagc tcgccgtccc cggcaacgcc gtgctgttcg gcttcttcat
     1681 cgccctgacc tgcgcgctgt cgttcgccga ggagcgccgc agcggcacct ggcgccggct
     1741 gctcgcctcc cccgtcccgg cctggaagct gctcgccacc aagctcgtcc cctacgtcgc
     1801 cctcggcacg atccaggtcg gcctgctgtt cgcgatcggc gcgctcggct tcggcatgca
     1861 gatcggcggc agcatcgccg cgctcgccgt cctcaccgtc ggcgtcgcgg cctgcgcgac
     1921 caccctcggc ctcttgatcg cggcgctgtc gtcgaccgag aagcagatct cgtcgctggg
     1981 ctcggtcctg atcatgatca tgggcctggt cggcggctgc atgttcccgc gcgccctgat
     2041 gccgcatgcc ctgcagcagg tcggcctgtt cgtcccccac ggctgggcgc tcgagggcta
     2101 ctacaccctc ctgatcggcc acggcaccgg cttcgccgac gtgctccgcc cgctcggcgc
     2161 cgtctacggc ttcgtgctgc tgttcgccgt cgccggcttg cgccggttcc gcttcgacca
     2221 gtaggcgccg gaactccctc ggctgtacac gctgcttgtc cctgacgaag cagcgcgttc
     2281 ccggcggggc atgcacccgc gtgcacgctc ctgttgagcg cggccgcatt gccccacggc
     2341 ggccatcgct gggttcgcgt ccgcctgtgg agcacgccgg agccacgagc acacgtcgag
     2401 gtccgcgccg tgcgctggag gaacggatcc cacgacatcg agaccgccga gctgacccgc
     2461 ggcgaagccg agctcgccga ggcatcgcgg tcggagctcg tcgctctcct ggcggccccc
     2521 ggggccgcgg cgctcgccca ggtggaaagc accgtgaggg atggcctgcg ctgcagggtg
     2581 acggtcctgg accgagccgc cgaccgcgtg caggagggat cctgcaacct cgccggcgta
     2641 cccgacgcgg cccgcgacca ccccagcgtg agactcgtgc aggccgtgct gcgcgccggg
     2701 gccgcggtcc cggtccccga gctgat
//
LOCUS       DASVOR010000473         1732 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_33427,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000473 DASVOR010000000
VERSION     DASVOR010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1732
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33427"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VF516_09420"
     CDS             <1..1472
                     /locus_tag="VF516_09420"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX8107934.1"
                     /translation="DELDAVAGINLSLEATPRIARRAAWLVTDSAYKEALIQLRAKLE
                     ARKTSGGHPDLPGWTTEKPVVGEEAVLVPPLETLDELETRARALSAAFRDQPAIRESR
                     VAITSYLERRWYLTTEGTSVTDTRRASGVVIAATGQADDGQLVHQYFLRYGQTAKDLP
                     SEDELKGETRKLVDSIVALQKAPVLDHYTGPVLFEGEGAVGLIRSALAPHLGGTPVPE
                     GLNPQEAKAFGGQLTDKVGLKVLAPSLTIVDDPTTHEGAGKALIGGYKIDDEGVAGQR
                     VEVIKDGMLKTLLTSRTPSAKNQASNGHARRIAEGGAFHGSTTNLFVSGKGAVPRKAL
                     EQRLVGAAQGEGLKYGIVIRRFDDAAITSAPEFARRELYAMIKSTDPHLPPPTLVAYK
                     VYPNGKEELVRGVQLAEVPIRAWKDVLGVSKEMTAYNFLAANESQLQLRISPTEDGFV
                     PSSGIESSIVTPDLLLKELDLGGSAAGERPLPLLPKPGK"
     gene            1469..>1732
                     /locus_tag="VF516_09425"
     CDS             1469..>1732
                     /locus_tag="VF516_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HEX8107935.1"
                     /translation="MTATEGNQAQAQAQAQIEVRIHAFEPRSRANGPGARFVVWFQGC
                     TLGCPGCFNPTTHDPDPGRAGGRTMPLDALVGELARARPGIEGL"
BASE COUNT          277 a          661 c          597 g          197 t
ORIGIN      
        1 ccgacgagct cgacgcggtc gccggcatca acctgtcgct cgaggccacg ccgcggatcg
       61 cccggcgcgc cgcctggctg gtcaccgaca gcgcctacaa ggaggcgctg atccagctgc
      121 gcgccaagct cgaggcgcgc aagacctcgg gcggccaccc cgacctcccg ggctggacca
      181 ccgagaagcc ggtggtcggc gaggaggccg tgctggtccc gccgctcgag acgctcgacg
      241 agctcgagac ccgcgccagg gcgctgtcgg ccgcgttccg cgatcagccg gcgatccgcg
      301 agtcgcgggt cgcgatcacc agctacctcg agcgccgctg gtacctcacc accgagggca
      361 ccagcgtcac cgacacccgg cgcgccagcg gcgtcgtgat cgcggcgacc ggccaggccg
      421 acgacggcca gctggtgcac cagtacttcc tgcgctacgg ccagaccgcc aaggacctgc
      481 cgagcgagga cgagctcaag ggcgagaccc ggaagctggt cgacagcatc gtcgcgctgc
      541 agaaggcgcc ggtgctggat cactacaccg gcccggtgct gttcgagggc gagggcgcgg
      601 tcggcctgat ccgctcggcc ctggcgcccc acctcggcgg aacgccggtg ccggaaggcc
      661 tcaacccgca ggaggccaag gcgttcggcg gccagctcac cgacaaggtc ggcctcaagg
      721 tgctcgcgcc cagcctgacg atcgtcgacg acccgacgac ccacgagggc gccggcaagg
      781 cgctgatcgg cggctacaag atcgacgacg agggcgtcgc cgggcagcgc gtcgaggtga
      841 tcaaggacgg catgctcaag accctgctca cgtcgcgcac gccgtcggcc aagaaccagg
      901 cctccaacgg ccacgcccgg cggatcgccg agggcggcgc gttccacggc agcacgacca
      961 acctgttcgt gtccggcaag ggcgcggtgc cgcgcaaggc gctcgagcag cgcctggtcg
     1021 gcgcggccca gggcgagggc ctcaagtacg gcatcgtgat ccgccggttc gacgacgccg
     1081 cgatcacctc ggcgcccgag ttcgcccggc gcgagctgta cgcgatgatc aagagcaccg
     1141 acccgcacct gccgcccccg accctggtcg cctacaaggt ctaccccaac ggcaaggagg
     1201 agctggtccg cggcgtccag ctcgccgagg tgccgatccg cgcctggaag gacgtgctcg
     1261 gcgtgtcgaa ggagatgacg gcgtacaact tcctcgccgc caacgagagc cagctccagc
     1321 tccggatcag ccccaccgag gatggcttcg tgccgtcgag cgggatcgag agcagcatcg
     1381 tcacgcccga tctcctgctc aaggagctcg acctcggcgg ctcggcggcc ggcgagcgcc
     1441 cgctcccgct cttgcccaag cccggcaagt gaccgcgacg gagggcaacc aggcccaggc
     1501 ccaggcccag gcccagatcg aggtccggat ccacgcgttc gagccgcgca gccgcgccaa
     1561 cggcccgggc gcacggttcg tcgtgtggtt ccagggctgc acgctcggct gccccggctg
     1621 cttcaacccg accacccacg atcccgaccc cggccgcgcc ggcggccgca cgatgccgct
     1681 cgacgcgctg gtcggcgagc tcgcccgcgc ccgcccgggc atcgagggcc tg
//
LOCUS       DASVOR010000474         6684 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_121228,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000474 DASVOR010000000
VERSION     DASVOR010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6684
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121228"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5164)
                     /locus_tag="VF516_09430"
     CDS             complement(<1..5164)
                     /locus_tag="VF516_09430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8107936.1"
                     /translation="MTDSAFPYRVTELLHNGGRLSLYRALREPDRVPVILKVLDPRRS
                     CPRDLAQLKNEYALGTSLDTEAVVKPLSLEIDREAPTLVLADFGGEPLSRLLGAPMAL
                     DRFLPLAVRIAEALAALHQQEIIHKDLKPQNILIHPRTSEIKLSDLGLASRLPREAQP
                     PCAAELVEGSLPYLSPEQTGRMNRALDNRADLYSLGVTLYELLTGRLPFEAGDPLEWL
                     HCHIARIPRSPTEIVPEVPPAVSAIVMKLLAKMPEERYQTARGLYRDLTRCAAQWRDC
                     GRIDRFPLAERDLSDRFQIPHKLYGRDHELAALLHAFERVVDSGAPELVLVSGYSGIG
                     KSALVRELHEPVVRAGGFFLSGKFDQYKRDIPYSTVVQAFQELVLELLAESEDRLAVW
                     RQRLLDELGVNAQLIIDVLPSLELVIGPQPAVPELPSIEAQNRFRLALQSFVGVFAQH
                     DHPLALFLDDLQWADPASVVLLQDLVMPSAARHLLVIGAYRDNEVSPVHPLMLMLDEM
                     HRTGTRITNVVLGPLSPQDLTALLSEALHCRPDDAAPLAELIHDKTAGNPFFAIQFLI
                     ALHDERLIQLDVQAERFRWDVDKIRAKGFTDNVVALMVDKVVRLPAATQAAIKRLACL
                     GNAADLDTLIAARGRSESEAESEAESGAEIHAALWDAVRAGLVVRTDRGYRFLHDRVQ
                     EAAYSLIPEDERAATHLRLGRALLAHLPEDERAERVFDLVSQLDRGVALITDPAEQAS
                     LCRFNVLAGNKAKAAIAYASARGYLAQAAALLPEDRWRTSHADTLALYLELAECEYLT
                     GHFQRADELFDLVLANARARRDRTAVHRLRMRLYQVSGRYDDAVSELLAALQLFGVTF
                     PESDPDLQAATEAELAAVPVHLAGRRIADLVDAPAITDPDQSAVISLIADSLSAVYIG
                     RPKYYILVNTRAVNLSLQYGHNAESCEAYSCYAISLIARFGDTRSAYEFSEMSLRLNE
                     KLDDRRLRGMVLHIQGGMVNFWRRHIATDLPFLDRGFRACLEVGDLVYAGFIAAETVW
                     QVIEKGDPLGEALRCSQRYAAFARDSHNEPIHETIRIQQQFIALLRGATRHPTRFDDA
                     GFDETASLAKLTGATFGAGIAFFRIMKLIAAVTFGFHAEALEAARQAQPVLGAAMAMP
                     IEATYHFYHALALAALDPQERTPELRSMLDDELHRLTRWADSCPDNYRHRHALVAAEI
                     ARIDGRELDAARLYEQAIRSAQDSGFVQFEALANELAARFYQTRGLDRLADGYLREAR
                     ACYVRWECDGKVQQLDLRHPQLRHAPPPTPTATVAVRLDQLDLVSVVKASQTISGEIV
                     LDELIRTLFQVVLEQGGAQRGTLLFAHDGGFALEAEATLEHDRVVTRLLPSLPVASAP
                     QRLPISVVTYVERTKQRVLIDDAVTAPGKFAADPYLAQRRTRSVLCLPILRQAKVVGL
                     LYLENALVAGAFTPDRLVALELLASQAAISVENALLLSKERAARALAEAARVHAEQAE
                     RRAEFLAEAGAILGESLVYEETLARLVRLCVRSLADGCVIDLVRNDGTIQRVAFAHAD
                     PAREPLLKELQDRYPPSRDSPHPAAHVLRSGEPLLLAELSDDLLRRMTVDEHHLHLNQ
                     AIGIRSLMVLPLTARGETLGTLTLVAATPGRRFSPADLDLAVEVARRAAAAIDKARLY
                     REAQDAIRLRDEFLTVASHELRTPMTSLTLSLQTLLHAAPAGKALDPKSISGL"
     gene            6003..6452
                     /locus_tag="VF516_09435"
     CDS             6003..6452
                     /locus_tag="VF516_09435"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8107937.1"
                     /translation="MAAKSKRKRKRHVQQALFRRGGKRRGAGRKPKGPRARERHAARP
                     AFKPYHPLHVVMRIVPEVGSLRRRKMYQAMRHATVTAALRERVRIVHISLQRNHVHML
                     VEADHKAALARGMQGFQISAARTSTPRLEMACGGAAARCSPTAITSR"
     gene            6449..>6684
                     /locus_tag="VF516_09440"
     CDS             6449..>6684
                     /locus_tag="VF516_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107938.1"
                     /translation="MITTPTRAHHALGYVLSNWRHHNEDQDGLPSTWLVDPFSSGISF
                     PDWKELDGQAWMWPMRATYDPMMVRRPQTWLLREG"
BASE COUNT         1087 a         2217 c         2405 g          975 t
ORIGIN      
        1 cgaggccgga gatcgacttc ggatcgagcg ccttgccggc gggggcggcg tgcagcaagg
       61 tctggagcga cagcgtgagc gaggtcatcg gcgtgcgcag ctcgtgcgag gccacggtca
      121 gaaactcgtc gcggagccgg atcgcgtcct gggcttcgcg atagagccgc gccttgtcga
      181 tcgcggccgc cgcgcggcgg gcgacctcga cggcgagatc caggtccgcg gggctgaacc
      241 ggcgacccgg cgtcgcggcg accagggtca gagttcccag cgtctcgccg cgcgccgtga
      301 gcggcagcac catcagcgac ctgatcccga tcgcctggtt caggtgcagg tggtgctcgt
      361 cgacggtcat ccgccgcagc aggtcgtccg agagctccgc caggagcagc ggctcacccg
      421 agcgcagcac gtgggcggcg gggtgcggcg aatcgcggct cggcggatag cggtcctgga
      481 gctccttgag caggggctcc ctggccgggt cggcgtgcgc gaacgccacc cgctggatcg
      541 tgccgtcgtt gcgcaccagg tcgatgacgc agccgtcggc gagcgagcgc acgcacagcc
      601 ggacgagccg ggcgagcgtc tcctcgtaga ccagcgattc accgaggatc gcgccggcct
      661 cggcgaggaa ctccgcgcgc cgctcggcct gctcggcgtg cacccgcgcg gcctcggcga
      721 gcgcccgcgc ggcgcgctcc ttcgagagca gcagcgcgtt ctcgaccgag atcgcggcct
      781 gggacgcgag cagctcgagg gcgaccagcc ggtcgggtgt gaacgcgccg gcgacgagcg
      841 cgttctcgag gtagagcagg ccgacgacct tggcctggcg caggatcggc aggcacagca
      901 ccgaccgcgt gcggcgctgc gcgaggtacg gatccgcggc gaacttgccc ggcgcggtga
      961 cggcgtcgtc gatcagcacg cgctgcttgg tgcgctcgac gtaggtcacg accgagatcg
     1021 gcagccgctg cggcgccgac gccaccggca gcgacggcag cagccgggtg acgacccggt
     1081 cgtgctcgag cgtggcctcc gcctcgagcg cgaagccgcc atcgtgcgcg aacagcaggg
     1141 taccgcgctg cgcgccgcct tgctcgagca cgacctggaa cagcgtgcgg atcagctcgt
     1201 cgagcacgat ctcgccggag atcgtctgcg acgccttgac caccgagacc aggtcgagct
     1261 gatcgagccg gaccgcgacg gtcgcggtcg gcgtcggcgg cggcgcgtgg cgaagctgcg
     1321 gatgacgcag atcgagctgc tggaccttgc cgtcgcactc ccaccgcacg tagcaggcgc
     1381 gggcctcgcg cagatagcca tcggcgagcc ggtcgagccc gcgggtctgg tagaaccgcg
     1441 cggcgagctc gttggcgagg gcctcgaact ggacgaagcc gctgtcctgc gccgaccgga
     1501 tcgcctgctc gtagagccgc gcggcgtcga gctcgcggcc gtcgatgcgc gcgatctcgg
     1561 ccgccaccag cgcgtggcgg tgccggtagt tgtcggggca gctgtccgcc caccgcgtca
     1621 gccggtggag ctcgtcgtcg agcatcgacc ggagctccgg cgtccgctcc tgcggatcga
     1681 gcgccgccag cgccagcgcg tgatagaagt gatacgtggc ctcgatcggc atcgccatcg
     1741 ccgcacccag cacgggctga gcttgccgcg cggcctcgag cgcctcggcg tggaacccga
     1801 acgtgaccgc ggcgatcagc ttcatgatgc ggaagaacgc gatgccggcg ccgaacgtcg
     1861 ccccggtgag cttcgcgagc gacgcggtct cgtcgaaccc ggcgtcgtcg aagcgggtcg
     1921 gatgacgcgt cgctcctcgg agcagggcga tgaactgttg ctggatccgg atcgtctcgt
     1981 ggatcggctc gttgtggctg tccctcgcga acgcggcgta ccgctgagag caccgcagcg
     2041 cctcgccgag cggatcgccc ttctcgatca cctgccagac cgtctcggcc gcgatgaacc
     2101 ccgcgtacac cagatcgccg acctcgaggc acgcccggaa gccgcggtcg aggaacggca
     2161 agtcggtcgc gatgtggcgg cgccagaagt tgaccatccc gccctggatg tggagcacca
     2221 tgccacgcag ccggcggtcg tcgagcttct cgttgaggcg cagcgacatc tcggagaact
     2281 cgtacgccga tcgcgtgtcg ccgaacctcg cgatcaagct gatcgcgtag caactgtacg
     2341 cctcgcacga ttcggcgttg tggccgtact ggagcgagag gttgaccgcc cgcgtgttga
     2401 ccaggatgta gtacttcgga cgtccgatgt agaccgcgga cagcgagtcg gcgatcaggc
     2461 tgatcaccgc gctctgatcg gggtcggtga tggccggggc gtcgacgagg tcggcgatcc
     2521 ggcggcccgc gaggtggacg ggaaccgcgg cgagctcggc ctcggtcgcg gcctggaggt
     2581 ccggatcgga ctcggggaac gtcaccccga acagctgcag cgccgcgagc agctcggaca
     2641 ccgcgtcgtc gtaccggccc gacacctggt acagccgcat gcgcaggcga tggaccgcgg
     2701 tgcgatcgcg gcgcgcgcgg gcgttggcga gcaccaggtc gaacagctcg tcggcgcgct
     2761 gaaagtgtcc ggtcaggtac tcgcactcgg cgagctccag atagagcgcg agcgtgtccg
     2821 cgtggctcgt gcgccagcgg tcctccggca ggagcgccgc ggcctgggcc agatagcccc
     2881 gcgccgacgc gtacgcgatc gcggccttgg ccttgttccc ggccagcacg ttgaaccggc
     2941 acagcgacgc ctgctcggcc ggatcggtga tcagcgcgac gccgcggtcg agctggctca
     3001 ccagatcgaa cacccgctcc gcgcgctcgt cctcgggcag gtgcgcgagc agcgcccggc
     3061 cgagccggag gtgggtggcc gcgcgctcgt cctcggggat cagcgagtac gcggcctcct
     3121 ggacgcggtc gtggaggaac cggtagccgc gatccgtgcg cacgacgagc ccggcgcgga
     3181 cggcgtccca cagcgcggca tggatctcgg caccggactc ggcctcggac tcggcctcgg
     3241 actcggagcg gccgcgggcg gcgatcagcg tgtcgagatc cgcggcgttg cccaggcagg
     3301 cgaggcgttt gatcgccgcc tgggtcgcgg cgggcaggcg gaccaccttg tccaccatca
     3361 gcgcgaccac gttgtcggtg aagcccttcg cgcggatctt gtcgacgtcc cagcgaaatc
     3421 gctctgcctg cacgtcgagc tggatcaagc gctcgtcgtg aagcgcgatc aggaactgga
     3481 tcgcgaagaa cggattcccg gcggtcttgt cgtggatgag ctccgcgagc ggagcggcgt
     3541 cgtccgggcg gcagtggagg gcctcgctca gcagcgcggt gaggtcctgc ggcgacagcg
     3601 gaccgagcac gacgttcgtg atccgcgtcc cggtccggtg catctcgtcg agcatgagca
     3661 tcagcgggtg gaccggcgat acctcgttgt cgcgataggc gccgatcacg agcaggtgac
     3721 gcgccgcgga cggcatcacc aggtcctgga gcagcacgac gctcgccggg tcggcccact
     3781 ggaggtcgtc gaggaacagc gcgagcgggt ggtcgtgctg ggcgaacaca ccgacgaagg
     3841 attgcagcgc gaggcggaac cggttctgcg cctcgatgga cgggagctcg gggaccgcgg
     3901 gctgcggccc gatcacgagc tcgagcgacg gcagcacgtc gatgatcagc tgggcgttga
     3961 cgccgagctc gtcgagcagg cgctgccgcc acaccgcgag ccggtcctcg ctctcggcga
     4021 gcagctcgag caccagctcc tggaacgcct ggacgaccgt ggagtacggg atgtcgcgct
     4081 tgtattgatc gaacttgccc gacaggaaga agccccccgc gcgcacgacc ggctcgtgga
     4141 gctcccggac cagggcggac ttgccgatgc ccgagtagcc cgacaccagc accagctccg
     4201 gggcgccgga gtcgaccacg cgctcgaacg cgtgcagcag cgcggcgagc tcgtggtcgc
     4261 gcccgtacag cttgtgcggg atctggaacc gatccgagag gtcgcgctcg gcgagtggga
     4321 accgatcgat ccggccgcag tcacgccact gcgcggcgca gcgggtgagg tcgcggtaca
     4381 ggccgcgcgc ggtctggtag cgctcctcgg gcatcttggc gagcagcttc atcacgatcg
     4441 ccgacacggc cggcggcacc tcgggaacga tctcggtggg cgagcgcggg atgcgcgcga
     4501 tgtggcagtg cagccactcg agcggatcgc cggcttcgaa cggcagccgt ccggtgagca
     4561 gctcgtagag cgtcacgccc agggagtaga gatcggcgcg gttgtcgagc gcgcggttca
     4621 tccgcccggt ctgctccggc gacaggtagg gcagcgagcc ctcgaccagc tccgcggcgc
     4681 acgggggctg cgcctcgcga ggcaggcgcg acgcaagccc gaggtccgag agcttgatct
     4741 cggaggtccg gggatggatc aggatgttct gcggcttgag atccttgtgg atgatctcct
     4801 gctgatggag tgcggcgagc gcctcggcga tgcggaccgc gagcggcaga aaccgatcga
     4861 gcgccatcgg cgcgccgagc aggcgagaca gcggttcgcc gccgaaatcg gcgagcacga
     4921 gcgtgggcgc ctcgcgatcg atctcgaggg acagcggctt gaccacggcc tcggtgtcga
     4981 gcgacgtgcc gagcgcgtat tcgttcttga gctgagcgag atcgcgcgga cagctgcgtc
     5041 gcggatcgag caccttgagg atcaccggga cgcgatcggg ctcacggagc gcgcggtaga
     5101 gactgaggcg accgccgttg tgcaggagct cggtgacccg atagggaaag gcgctgtccg
     5161 tcatggaccc tcggcggccg ccgcgccggc atcgtcatgt caccagaaac ggtgactcga
     5221 gactcgaacg taccacgccg aggcagcgca gcaccgacgc cggcgccgcc ggcccgcgcg
     5281 cctgcgagaa accgaccgtg cgtcggcgcg cacccggcgc tgacgacaac gactgcgact
     5341 ccggtgcatg cggtagcagc ggctcgcccg agccctgtca cagtgaaaca aggagggacc
     5401 tcgggagaac ccatggtcgc ctgtgactgg cgcgccgcgg ctcgccaggt tgacctggac
     5461 gtcccactca cagccggctg ccctgcgcgc atgccacctt tccggagatt tgattttcgg
     5521 ggggggattt ttcgtacact cgccgagata tacaatctag cgttgcgttg accgattgaa
     5581 ctcacgtccc aaaagaaaaa ataaactctt caagggagcc tgggatcgag ggtcagcact
     5641 gtggggaacg gcggaggact gtgagccgcg ccctggtcga cctgatgcac atcagcgcgg
     5701 gtcatcaggc cgacccgttg tccacagggc cgcccccgag ttctgcggaa gttccgctcg
     5761 aggctcgtcg acgctggcac gatcaggcag atcacagctg acgatcgatg gcgcgtgctc
     5821 aaagcgcaac gagtcagtgc cgcgtgttcg atccgggaca atctgtatcc gattgagcac
     5881 gactgtcacc aaatcgatac ttcgcaccgc gttctttcga atctcacaag tcatcaatca
     5941 tgaacgatat tacttatgcc caatcaattg acatcgtgca cgtccatgat aacaaggagt
     6001 ccatggcggc gaagtcgaag cggaagcgca agcggcatgt ccagcaggcg ttgttccggc
     6061 gcggcggcaa gcggcgcggt gccgggcgaa agcccaaggg tccgcgtgcc agggagcgcc
     6121 acgcggcgcg accggcgttc aagccgtacc acccgctgca tgtcgtgatg cggatcgtgc
     6181 cggaggtcgg gagcctgcgg cgacgcaaga tgtaccaggc gatgcggcac gccaccgtca
     6241 ctgccgcgct acgcgagcga gtccggatcg tccacatctc cttgcaacgc aaccatgttc
     6301 acatgctggt cgaggccgat cacaaggccg cgctcgctcg cggtatgcag ggcttccaga
     6361 tctcggcggc gcgaacatca acaccgcgct tggagatggc gtgcggcggc gccgcggcaa
     6421 ggtgttcgcc gaccgctatc acgtcgaggt gatcacgacg ccgacgcggg cgcatcacgc
     6481 tcttgggtac gtgctttcca actggcgcca ccacaacgag gaccaggacg gcttgccgag
     6541 cacatggctc gtcgatccgt tctcttccgg catctcattc cccgactgga aggagctgga
     6601 cggccaggcc tggatgtggc cgatgcgcgc gacctatgac ccgatgatgg tgcggagacc
     6661 gcagacctgg ttgctccgcg aggg
//
LOCUS       DASVOR010000475         1873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501259,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000475 DASVOR010000000
VERSION     DASVOR010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1873
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501259"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..874)
                     /locus_tag="VF516_09445"
     CDS             complement(<1..874)
                     /locus_tag="VF516_09445"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8107939.1"
                     /translation="MTILVLGATGFVGRALVAALSDANETVRAASRTVPRRSVDSERP
                     GVRWIYCDLARPETLPPALEGVDVAYYLVHSMGGHSLGGHGGDFREIDRRCARAFARA
                     AAHSSCRRIIYLGGVAPRRRPSEHLASRLEVGEILRAGTVPALELRASMIVGNGSASW
                     QIVRDLAERLPFMILPRWLDTRTCPIALPDAIAALLDARDVPLPASAWFDIPGPEVLR
                     AREMLMAVAALDGRRVPYVRVPVLTPGLSAMWLKLVSGVDYTLARELVLGLTHDLLPQ
                     RSFWAITGRAPRWRF"
     gene            995..>1873
                     /locus_tag="VF516_09450"
     CDS             995..>1873
                     /locus_tag="VF516_09450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8107940.1"
                     /translation="MPQPRQLSEADLEVLRRIERLPFGRFQWRMLWMGGLGYTFDAMD
                     GALIAFILPAVTKLWHLSSEDTGLVGSSAMIGYLFGAFFAGALGDLIGRRTVMMYGLA
                     IYSVATLIAAFAPSWQVLFWWRVAASVGTGVEGAIIAPFLAEFVAKQHRGRFIGSLAG
                     FFSYGFVFAALLGYFVIPASPDGWRIVQVISALPIAMLLWWRRSLPESPRWLIERGRS
                     AQARAEVERIEAELTRGGRTLPPADVVELPAASARGGSDLAASARGGSFLDNLAALRG
                     RPVRRTTVMLWILWIAI"
BASE COUNT          261 a          656 c          691 g          265 t
ORIGIN      
        1 ggaaccgcca gcgcggcgcg cggccggtga tggcccagaa gctgcgctgg gggagcaggt
       61 cgtgggtgag cccgaggacg agctcgcgcg cgagcgtgta atcgaccccc gacacgagct
      121 tgagccacat cgcggacagc cccggcgtga gcaccgggac gcggacgtac gggacacggc
      181 gaccgtcgag cgcggcgacc gccatgagca tctcccgcgc gcgcaggacc tcggggcccg
      241 ggatatcgaa ccacgcgctc gcggggagcg ggacgtcccg cgcgtccagg agcgcggcga
      301 tcgcatcggg cagcgcgatc ggacaggtcc tcgtgtcgag ccacctcggg aggatcatga
      361 acggcaggcg ctcggccaga tcgcgcacga tctgccacga tgcactcccg ttgccgacga
      421 tcatcgaggc gcgcagctcc agcgccggga ccgtcccggc tcgcaggatc tcgccgacct
      481 cgagccggct cgcgaggtgc tccgacggcc ggcgccgcgg ggccacgccg ccgaggtaga
      541 tgatccgccg gcagctgctg tgcgccgcgg cgcgggcgaa cgccctcgcg catcgccgat
      601 cgatctcgcg gaagtcgccg ccgtgcccac ccaggctgtg cccacccatg ctgtggacga
      661 ggtagtacgc gacgtcgacg ccctcgagcg ccggtgggag ggtctcgggt cgagcgaggt
      721 cgcagtaaat ccagcgcacc ccgggccgct cgctgtccac ggatcgacga ggcacggtgc
      781 gcgaggcagc ccgcacggtc tcgttcgcgt cggacagcgc ggcgaccagc gcccggccga
      841 cgaacccggt ggcgcccaga acgaggatgg tcatggcacg aaccctccag acatcgggtt
      901 ggcatgttgc aaacccggcg ccgtaacgcc gcggttgcgg tcgcgccgcg atcggcgcga
      961 ggatgaggcc accgcacgtc ccggagggcc gacgatgccg cagccgcgcc agctcagtga
     1021 agccgatctc gaggtcctgc ggcgcatcga gcgcctgccg ttcggccgct tccagtggcg
     1081 aatgctgtgg atgggcggcc tgggatacac gttcgatgcg atggacgggg cgctgatcgc
     1141 gttcatcctc cccgcggtca ccaagctgtg gcacctgagc agcgaggaca ccggcctggt
     1201 gggcagctcg gcgatgatcg ggtatctgtt cggggcgttc ttcgccggcg cgctcggcga
     1261 cctgatcgga cggcggaccg tcatgatgta cggcctggcg atctacagcg tggcgacgct
     1321 gatcgcggcg tttgccccga gctggcaggt actgttctgg tggcgggtcg cggcgagcgt
     1381 cggcaccggc gtcgagggcg cgatcatcgc cccgttcctc gcggagttcg tcgccaagca
     1441 gcatcgcggg cggttcatcg gctcgctggc cggcttcttc tcgtacggct tcgtgttcgc
     1501 ggccctgctc ggatacttcg tcatcccggc ctctccggac ggctggcgga tcgtgcaggt
     1561 gatcagcgcg ctgccgatcg ccatgctgct gtggtggcgc cggtcgctgc ccgagtcgcc
     1621 gcgctggctg atcgagcgcg ggcggtcggc gcaggcccgc gccgaggtcg agcgcatcga
     1681 ggccgagctg acgcgcggcg gccgtaccct gcctccggcc gacgtcgtcg agctccccgc
     1741 cgccagcgca cggggcggca gcgaccttgc cgccagcgca cgcggcggaa gcttcctcga
     1801 caacctggcc gcgctgaggg gccgcccagt gcggcggacc accgtcatgc tgtggatcct
     1861 gtggatcgcg atc
//
LOCUS       DASVOR010000476         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_412959,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000476 DASVOR010000000
VERSION     DASVOR010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_412959"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..361)
                     /locus_tag="VF516_09455"
     CDS             complement(83..361)
                     /locus_tag="VF516_09455"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8107941.1"
                     /translation="MPGSPARLDAEIASKLATSRQTLYRALKAEGATFEQVLDDLRHR
                     MALHYLRGKKVSVNECAYLVGVSDPTAFSRAFKRWTGTSPRAIRASMK"
     gene            complement(351..689)
                     /locus_tag="VF516_09460"
     CDS             complement(351..689)
                     /locus_tag="VF516_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107942.1"
                     /translation="MACHRPSPATAGSPERTAAASCKVSKPVCDPRVTEDTALTLVRR
                     RCAGCHAEGGKADHPFLDADALSATRGDVALRLAGCEMPPDDTFLPPEERARLIGWGA
                     CVLPAAGAAR"
     gene            complement(727..1647)
                     /locus_tag="VF516_09465"
     CDS             complement(727..1647)
                     /locus_tag="VF516_09465"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8107943.1"
                     /translation="MSESSRRDLFRLAGGALWVGAVSACAGAGASQRATTPAAGGGAA
                     AGAEDAGGFLMFQITDTHWGYKGAANPNPGAAFERAVAEIARWPTRPALVVHTGDVTH
                     MTTDAGERKARLETAKAMLAKLGSELLVIPGEHDAGADHGAAFQAVFGPTHHARELRG
                     VSLIAIDNVSDPRGAIGDEQLAWLDKEVAKVPADKPLLVFTHRPLFQLAQPWDWFTQD
                     GDKALAILDRHPNTTVFYGHIHQALIHKTNSTLHVSTRSLVFPLPAPMSQPAKAPLPW
                     DADALDHGLGYRGIAVAGAQPTWVERALVG"
     gene            1877..2545
                     /locus_tag="VF516_09470"
     CDS             1877..2545
                     /locus_tag="VF516_09470"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8107944.1"
                     /translation="MTTHIGMLLFPRLTQLDLTGPFEVLHRLPDTKVHLVWKDTQPVY
                     ADSGLGLVPTLSFADCPALDIVFVPGGLGQVALMTDPIVLDFLRHHGRTARYVTSVCT
                     GALVLGAAGLLDGYRAATHWAFVELLPVFGARHEPGRVVVDRNRITGGGVTAGIDFAL
                     RLAAELAGEDVARSIQLGLEYDPAPPFASGHPDVADPALVARVRERIGPLRKRLGGEA
                     PAAR"
     gene            2558..3304
                     /locus_tag="VF516_09475"
     CDS             2558..3304
                     /locus_tag="VF516_09475"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HEX8107945.1"
                     /translation="MLKDKVIVITGASRGIGEAIARAAVDAGARVALASRKQADLDQV
                     ASSLPADRAIALACHTGKPDDVDAMFRKAVERFGRVDGLVNNAATNPYFGPLIDTPDA
                     AIDKTFEVNVKGYLYCARALVRHARERGGGGAIVNIASIAGMRAAIMQGIYGATKAAV
                     ISMTQTLAAELGGSGIRVNAIAPGLVETRFASALVNNPAILKQVVSRTPLGRHAQPPE
                     LAGAAIYLLSDAASFTTGTTLVVDGGVTAG"
     gene            complement(3369..3599)
                     /locus_tag="VF516_09480"
     CDS             complement(3369..3599)
                     /locus_tag="VF516_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107946.1"
                     /translation="MCGGDGQNPPQKVDGRDVAEVTGAAVRSTLNPLSVLGNVPDAIN
                     RVTRTDRVGNNVGDRLLDGFTGLFGIDPLKKR"
     gene            3751..>4064
                     /locus_tag="VF516_09485"
     CDS             3751..>4064
                     /locus_tag="VF516_09485"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107947.1"
                     /translation="MDDVAERDQRSKRLIELASCRARAADRIARRSMYSRWRATRRGA
                     GGRGLVRRIGTASAGRINIEIFPAGVLVRPFETFDAVGAGVADMYHSAEYYWEKKSPA
                     FS"
BASE COUNT          578 a         1427 c         1456 g          603 t
ORIGIN      
        1 ctcgaggccc gcctgggcag cgcctcgggc gagaaggaat gaccagccca cgtcctcgct
       61 gcgcgcgccg agccgtgggg cgtcacttca tcgacgcgcg gatcgcgcgg ggactcgtgc
      121 cggtccaccg cttgaacgcg cgggagaacg cggtcggatc cgagaccccg acgagatacg
      181 cgcactcgtt gaccgagacc ttctttcccc gcaggtaatg aagcgccatc ctgtggcgga
      241 ggtcgtcgag cacctgctcg aaggtcgcgc cctcggcctt caacgcgcga tacagcgtct
      301 gccggctcgt ggcgagcttg ctcgcgatct cggcgtcgag gcgcgcgggg ctacctggca
      361 gcgccggcgg cgggcagcac gcaggcgccc cagccgatca accgggcgcg ctcctcggga
      421 ggcaggaacg tatcgtcggg aggcatctcg cagccggcga ggcgaagcgc gacgtcgccg
      481 cgggtcgcgg acagcgcatc ggcgtcgagg aaggggtgat cggccttgcc gccctcggcg
      541 tggcagcccg cgcagcgccg gcggaccagc gtcagcgcgg tgtcctcggt gacccgcgga
      601 tcgcacaccg gcttcgacac cttgcagctc gcggcggcgg tgcgctcggg cgagccggcg
      661 gtggccggcg acgggcggtg gcaggccacg gcagagacga ctgtgagggc ggaaaggaac
      721 gcgaggctaa ccgacgaggg cacgctcgac ccaggtcggt tgcgcgccgg cgaccgcgat
      781 gccgcggtag cccaggccgt gatcgagcgc atcggcgtcc cacggcagtg gggccttcgc
      841 cggctgcgac atcggagccg gcagcggaaa taccagcgag cgggtcgaga catgcagcgt
      901 gctgttcgtc ttgtggatca gggcctggtg gatgtggccg tagaataccg tcgtgttcgg
      961 gtggcggtcg aggatcgcga gcgccttgtc gccgtcctgg gtgaaccagt cccacggctg
     1021 ggccagctgg aacagcggcc ggtgcgtgaa caccagcaac ggcttgtcgg ccgggacctt
     1081 ggcgacctcc ttgtcgagcc aggcgagctg ctcgtcaccg atggctcccc tggggtcgga
     1141 cacgttgtcg atcgcgatca gggagacgcc gcgcagctcg cgggcatggt gggtcgggcc
     1201 gaacacggcc tggaacgccg cgccgtggtc ggcgccggcg tcgtgctcgc cggggatgac
     1261 cagcagctcg ctgccgagct tggcgagcat cgccttggcg gtctcgagcc gggccttgcg
     1321 ctcgccggcg tcggtggtca tgtgggtgac gtcgccggtg tggacgacga gcgcgggcct
     1381 ggtcggccac cgcgcgatct cggcgaccgc gcgctcgaac gccgcgccgg ggttggggtt
     1441 ggccgcgccc ttgtagcccc agtgggtatc cgtgatctgg aacatcagga acccgcccgc
     1501 gtcctccgct cccgcggcgg cgcccccacc ggccgcggga gtcgtggcgc gctgcgacgc
     1561 accggctccg gcgcaggcgc tcaccgcgcc gacccacagc gcgccgccgg ccagacggaa
     1621 cagatctcga cgacttgact cgctcatcat tgcctcctgc gcggtcaacg agcgacgcgt
     1681 cgatcggttc ccggggcggg tgccaaggat ttcccttgca gcgcgccgcg cactcttcgg
     1741 agtacgcctg ggtgacagct cctccggcgg gagcagctgg gcgtgcagcc cgggactgcg
     1801 cccctcaccg cccgtggtgc cttcgaggct gatctttgtc atggctgcca cgggcctcga
     1861 gcgccacgat gccgccatga cgacgcacat cggcatgctg ctgttccccc ggctgaccca
     1921 gctcgacctc accggaccgt tcgaggtgct gcaccgcctc cccgacacca aggtccacct
     1981 ggtgtggaag gacacccagc cggtgtacgc cgactccggc ctcggcctgg tcccgaccct
     2041 gtcgttcgcc gactgcccgg cgctcgacat cgtgttcgtg cccggcggtc tcggccaggt
     2101 cgcgctgatg accgacccga tcgtgctcga cttcctgcgc caccacggca ggaccgcgag
     2161 gtacgtcacc tcggtgtgca ccggcgcgct ggtgctcggc gccgccggcc tgctcgacgg
     2221 ctaccgcgcc gcgacccact gggcgttcgt cgagctcttg ccggtgttcg gcgcccgcca
     2281 cgagcccggc cgggtcgtgg tcgaccgcaa ccggatcacc ggcggcgggg tcaccgccgg
     2341 gatcgacttc gcgctgcggc tcgccgccga gctcgccggc gaggacgtcg cccggtcgat
     2401 ccagctcggc ctcgagtacg accctgcccc gccgttcgcc agcggccacc ccgacgtcgc
     2461 cgacccggcc ctggtcgccc gggtgcgcga gcgcatcggc ccgctgagaa agcggctggg
     2521 cggtgaagcg cccgccgcgc ggtgatacgt tccgcgcgtg ctgaaagaca aggtcatcgt
     2581 catcaccggg gcgagccgcg ggatcggaga agcgatcgcg cgcgccgccg tcgacgcggg
     2641 tgcccgggtc gcgctcgcgt cgcgcaagca ggccgacctc gaccaggtcg cgagctcgct
     2701 gcctgccgac cgcgcgatcg cgctggcgtg ccacaccggc aagcccgacg acgtcgacgc
     2761 gatgtttcgc aaggcggtcg agcggttcgg ccgcgtcgac ggcctggtca acaacgcggc
     2821 gaccaacccg tacttcggcc cgctgatcga cacgccggac gcggcgatcg acaagacctt
     2881 cgaggtcaac gtcaagggtt acctgtactg cgcccgcgcc ctggtccgcc acgcccgcga
     2941 gcgcggcggc ggcggcgcga tcgtcaacat cgccagcatc gccggcatgc gcgccgcgat
     3001 catgcagggc atctacggcg ccaccaaggc cgcggtgatc agcatgaccc agaccctcgc
     3061 cgccgagctc ggcggcagcg gcatccgggt caacgcgatc gcccccggcc tggtcgagac
     3121 ccggttcgcc tcggcgctgg tcaacaaccc ggcgatcctg aagcaggtcg tcagccgcac
     3181 gccgctcggc cgccacgccc agccgcccga gctcgccggc gcggcgatct acctcctgtc
     3241 cgacgcggca tcgttcacca ccggcaccac gctggtcgtc gacggcggcg tgaccgcggg
     3301 ctgagcggct cgaggacgga tcacatcggt catggacgat cgtccctctg gttggagcgt
     3361 ggtgcggatc agcgcttctt gagcggatcg atcccgaaca ggccggtgaa gccgtcgagc
     3421 aggcgatcgc cgacgttgtt gccgacccgg tcggtgcggg tgacgcggtt gatcgcgtcg
     3481 gggacgttgc ccagcaccga gagcgggttc agcgtcgagc ggaccgcggc gccggtgacc
     3541 tcggcgacgt cgcggccgtc gaccttctgc ggcgggttct ggccgtcgcc accacacacg
     3601 atgtgaagat cctgagaggc aatcgcttcg aagttcgaca ttggcatttc tccttggttg
     3661 tgtccgtcga gagctccgac ggcgaaccca ggcaattgca gagggtgtgc cgcgatcagc
     3721 gaatggcgac cacgccgcga cgtcgcgagg ttggacgacg tcgcggagcg agaccagcgt
     3781 tcgaagcgtc tgatcgagtt ggcaagctgt cgcgcgcggg cggcagatcg catcgcgcgc
     3841 aggtcgatgt acagcaggtg gcgtgccacg cggcggggcg ccggcggccg tggcctggtg
     3901 cggaggattg gtaccgcgtc ggcagggcgc atcaacatcg agatatttcc cgctggcgtc
     3961 ctcgttcgtc cgtttgagac attcgatgca gtgggcgctg gtgtagccga catgtaccat
     4021 tccgccgaat actattggga gaagaaatcg ccagcgttca gctt
//
LOCUS       DASVOR010000477         5411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_150481,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000477 DASVOR010000000
VERSION     DASVOR010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150481"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1172
                     /locus_tag="VF516_09490"
     CDS             33..1172
                     /locus_tag="VF516_09490"
                     /inference="COORDINATES: protein motif:HMM:NF033668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEX8107948.1"
                     /translation="MKSRPTANPPNRFHDHVVTYDDGEAPPPAQVTLIDDRTRSILAH
                     NDSPDLGFRWSVNPYRGCQHACAYCYARPSHEYLDLGAGTDFDTKIVVKRDAAALLRE
                     AFDKPSWRGELVMFSGVTDCYQPVERELELTRRCLEVCLEYRNPVSVISKSALVERDL
                     DLFAALTEHARFGISVSLAFSDNATARAIEPWAPSPDRRFKLIETLAAAGLPVGVMCA
                     PIIPGVNDSQMVTVLERAAAAGATWAGWVLLRLPGAVKQVFEDRIRDAMPLAADKILH
                     RIRETRGGDKLYDPRFGVRGRGEGVYADMIRTMFDTIVARLHLNRRERTRDGMVDKRS
                     EETGGGGPAGSAGGAGGNAPRGIDEADVPTTFRRPAPTTGQLSLF"
     gene            complement(1333..3579)
                     /locus_tag="VF516_09495"
     CDS             complement(1333..3579)
                     /locus_tag="VF516_09495"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8107949.1"
                     /translation="MAAAAATRAPPRAAILAAATGSGAIDGIVVDERNVPVSGAAVLV
                     TSHDEVRSETDGSFHVAGVAPGRYYLNAHSETGAAGPLPLEVDEAPLSVVVRLHPGVS
                     LAVEVVSIVDGHPVPNARGSLLFSSMIGAAGHVETVAGADGILHFPVAALGNYHILAE
                     ADGFAPATRLLTWNDRAGLMWRALIALEPGVVLTGRVVDESGVPVRGARVTSRPPPRA
                     QTQMAYRPRPPDPLRPPVMTDSDGRFRYPVPVGQGLVLLASHEHYAGGDSGPLVATGP
                     ADIVIRLRAGQRLTGRVVDRDHRPVAQATVTDGAPESAPAHIARTDADGRFVMEGIDR
                     RRHDAILFAEAHGMRASPVLVSLPSGFDRPIELVVEHDLRAAGIVVDSSGVPVGDAIV
                     HFQHVVAPSYRPPPANPTAVLEPMVTGETRADASGAFVISGLGPGDVQLLARAPSAGG
                     DRASPPLTATTSAKAGDEKVVITLPAPARIRGRVVTASGRPPTGMEVSLLQFGGPLPV
                     ADPEGRFELSQIDPAPGTYSVKVTADGSAARTVEARVTAGQTTDLGIITLEAGRTLRG
                     RVLDAGGSGPVANALVSVDGPKGTRIASCRTDELGGFEVPVPNDPVMVHATVPGVGGS
                     RFELVAPTRNSVELRLPETGRIEVTVTGPAAAGALTVSATRTDAVDGGFRIFMLNRDP
                     GQPTFQADVSAGEYVVHASPGTGVAAMKGGDTQHDVAVAVAVGQIQKVTIATGGSGTS
                     PAQDGANR"
     gene            complement(3863..4432)
                     /locus_tag="VF516_09500"
     CDS             complement(3863..4432)
                     /locus_tag="VF516_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107950.1"
                     /translation="MSNSRTKWTLIFVLMVGLGTLSTTAHAQAGNWRWGPPGFSVGMS
                     PMDREYYLVNQGLADPYVNYWTPFWSGSSGPNVYTGVFDQGFRNTFAVNHDINANFGI
                     PILGYLDIFTPLFGTNELAIGSTVASVWNSIVSTVSSVWNSITSLFSRDYTSYVDTGG
                     YSDGDWDNAFDDGYNDYDCGCYGGDGYGG"
     gene            complement(4759..>5411)
                     /locus_tag="VF516_09505"
     CDS             complement(4759..>5411)
                     /locus_tag="VF516_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107951.1"
                     /translation="ARATFRLGVEPLGLDPSEDTPLVGGHVADAVAVYNAGSAAYNRL
                     HGYAPGSAMASATIDASALGLHTTLITFAPGVELGGDHVRFRLEGLASVSDRLHAFGL
                     AMYPLDLALPLSGGAVTPYLVAGGTLRWLDRSDVDGESGGLVTVRTAAGARIAGRIDV
                     ELGVGLYLLGGVYNGAQLQSMSSYDPRGNTPLPAPDRAVAGGQQSGMIDVSVGFAL"
BASE COUNT          888 a         1926 c         1806 g          791 t
ORIGIN      
        1 acagctatac atcggttcag tacactgcac gggtgaagtc ccggcccacc gcgaacccgc
       61 cgaaccggtt ccacgaccac gtcgtgacct acgacgacgg cgaggccccg ccgccggcgc
      121 aggtcaccct gatcgacgat cgcacccggt cgatcctcgc ccacaacgac tcccccgacc
      181 tcggcttccg ctggagcgtc aacccgtacc gcggctgcca gcacgcctgc gcctactgct
      241 acgcgcgccc cagccacgag tacctcgacc tcggcgccgg caccgacttc gacaccaaga
      301 tcgtcgtcaa gcgcgacgcg gcggcgctgc tccgcgaggc gttcgacaag ccgagctggc
      361 gcggcgagct ggtgatgttc tccggcgtca ccgactgcta ccagccggtc gagcgcgagc
      421 tcgagctgac ccggcgctgc ctcgaggtct gcctcgagta ccggaacccg gtcagcgtga
      481 tctccaagag cgcgctggtc gagcgcgacc tcgacttgtt cgccgcgctc accgagcacg
      541 cccggttcgg catcagcgtc agcctcgcgt tcagcgacaa cgccaccgcg cgcgcgatcg
      601 agccctgggc gccgtctccc gaccgccggt tcaagctgat cgagaccctc gccgcggccg
      661 gcctcccggt cggcgtgatg tgcgcgccga tcatccccgg cgtcaacgac agccagatgg
      721 tcaccgtgct cgagcgcgcc gccgccgcgg gcgcgacctg ggccggctgg gtcctgctcc
      781 gcctgcccgg tgcggtcaag caggtgttcg aggaccgcat ccgcgacgcg atgccgctcg
      841 ccgccgacaa gatcctgcac cgcatccgcg agacccgcgg cggcgacaag ctctacgacc
      901 cgcggttcgg cgtgcgcggc cgcggcgagg gcgtctacgc cgacatgatc cgcacgatgt
      961 tcgacaccat cgtggctcgc ctccacctca acagacggga gcgcacccgc gacggcatgg
     1021 tcgacaagcg aagcgaggag acggggggtg ggggccctgc cggctctgcc ggtggggcgg
     1081 ggggcaacgc cccccggggg atcgacgagg ccgacgtccc gaccacgttc cgccggcccg
     1141 cgccgaccac cggccagctg tcgctgttct agcccgcatc attccccacg acgtctcggt
     1201 cgatccactt ccgctcgctg cgctcgcggt ggcaagcggc gcgaggcaga tcggggcgca
     1261 cgcccgtttc ggtggacctc gggggccggc ggggctgctt cgcactgagg gtaatcgacg
     1321 agccagctct gactaacgat tcgcgccgtc ctgtgccggc gacgtccccg aacctccggt
     1381 cgcgatcgtc accttctgga tctgcccgac ggccactgcc accgcaacgt cgtgctgcgt
     1441 gtcgccgcct ttcatcgcgg caacccccgt tccggggctc gcgtgaacca cgtactcgcc
     1501 cgctgacacg tctgcctgga aggttggctg gcccgggtcg cggttcagca tgaagatgcg
     1561 aaagccaccg tcgacggcgt cggtccgagt cgcactgacg gtcagcgcac cggccgccgc
     1621 cggaccggtc acggtgacct cgatccggcc ggtctcgggc agacgaagct cgaccgagtt
     1681 ccgcgtgggg gccacgagct cgaagcgcga gccgccgacc ccgggtaccg tggcgtgcac
     1741 catcaccgga tcgttgggta cgggaacctc gaagccgccc agctcgtccg tgcggcaact
     1801 ggcaatgcgc gttcctttgg ggccgtccac cgagacgagc gcgttcgcaa ccgggccact
     1861 gccaccggcg tcgagcaccc gtccccgcaa cgtgcggcct gcctcgaggg tgatgatgcc
     1921 gaggtcggtc gtctgcccgg ccgtgacgcg cgcctcgacc gtccgtgcag cggagccatc
     1981 ggcggtgacc ttcaccgagt aggtgcccgg cgccgggtcg atctggctga gctcgaagcg
     2041 accttcaggg tcggcgacgg ggagcggtcc cccgaactgg aggagcgaca cctccatccc
     2101 ggtcggcggg cggcccgaag cagtgacgac ccgaccgcgg atccgcgccg gcgcggggag
     2161 cgtgatgacg accttctcgt caccggcctt ggcgctggtg gtcgccgtga gcggcggcga
     2221 cgcgcgatcc ccgcccgccg aaggtgcccg cgcgaggagc tgcacgtcac caggcccgag
     2281 gccggagatg acgaaggcgc cgctcgcgtc ggcgcgagtc tcgccggtca ccatgggctc
     2341 gaggacagcg gtcgggtttg cgggcggcgg ccggtacgat ggcgccacca cgtgctggaa
     2401 gtgaacgatc gcgtcgccga ccggcacgcc gctcgaatcg acgacgatgc cggcggcccg
     2461 gagatcgtgc tccacgacga gctcgatcgg ccggtcgaac ccgctgggga gcgagaccag
     2521 gaccgggctc gcccgcattc cgtgcgcctc cgcgaacagg atggcgtcgt gacgacgccg
     2581 atcgatgccc tccatcacga accggccatc ggcgtccgta cgggcgatat gggccggcgc
     2641 actctcgggg gcaccgtcgg tgacggtcgc ctgcgcgacc ggtcgatggt cgcggtcgac
     2701 gacccgcccg gtgagtctct ggccggcgcg cagcctgatc acgatatcgg ccgggccggt
     2761 ggcgacgagg ggcccgctgt cgccgccagc atagtgctcg tgacttgcca gaaggacgag
     2821 gccctgaccg acggggacgg gatagcgaaa ccggccgtcg gaatcggtca tgaccggcgg
     2881 tcgcagcgga tccggaggcc ggggtcggta ggccatctgg gtctgagcac gcggtggtgg
     2941 gcgcgatgtc acccgtgccc cacggaccgg gacgcccgac tcgtcgacca cacgaccggt
     3001 caagacgacg cccggctcga gtgcgatcag cgcgcgccac atgagccccg cgcggtcgtt
     3061 ccaggtcagg agccgtgtcg ccggggcgaa gccgtcggcc tcggcgagaa tgtgatagtt
     3121 gccgagtgcg gcgaccggaa aatgcagaat gccatcggca ccggcgacgg tttcgacgtg
     3181 accggccgcg ccgatcatgc tggagaagag gagggatccg cgcgcgttcg gcaccggatg
     3241 cccgtcgacg atagagacga cctcgaccgc caagctcacg ccgggatgga gccggaccac
     3301 gacggagagc ggcgcttcgt cgacctccag tgggaggggt cctgcggcgc cggtctcgga
     3361 gtgcgcattc aggtaataac ggccaggcgc gactccggcg acgtggaagc tgccgtcggt
     3421 ctcggagcga acctcgtcgt gcgaagtgac gaggacggcg gcaccggaga ccgggacatt
     3481 gcgctcgtca acaacgatgc cgtcgatcgc gccagatccc gtggctgccg ccaggatcgc
     3541 cgcgcgcggt ggcgcccgtg tcgccgccgc agccgccaac ctatccgagg gttgccccgc
     3601 ggccgaaccg cgctcgccct ccgggcgaac atccagccag aaccaaacca gcgcccccag
     3661 gatgagcgcg accagcgcac cgaccttcgt gacacgacgg gcctgggcgc gattgggttc
     3721 tcgagttggt gtccgcaagc cttcactccg ccttgctatg gccaggctga actcgctcgt
     3781 agcatccgag atcgctcttg cgcaccgatt cgatgaggcg ggtcgagccg ctggcgacgg
     3841 tgatgggtgc cccggctacg agtcagccac cataaccatc accaccataa cagccacaat
     3901 cgtaatcatt gtacccgtcg tcgaatgcat tgtcccaatc gccgtcggaa tagccacctg
     3961 tgtcaacata gctcgtatag tctctcgaga atagcgaggt gatagaattc cagaccgagg
     4021 acaccgtgct cacgatgctg ttccagaccg acgctaccgt cgatccgatc gccagctcgt
     4081 tcgtgccgaa aagcggagtg aagatgtcga gatatcccag gatcggaata ccaaaattgg
     4141 cattaatgtc atggttgaca gcgaaggtgt tacggaagcc ctgatcgaac actccggtgt
     4201 agacgttcgg gcccgagctg ccggaccaga acggcgtcca gtaattcacg tacggatcgg
     4261 ccaacccctg attgacgagg taatactcac gatccatggg gctcatcccc acgctgaagc
     4321 ctggcggccc ccacctccag tttccagcct gtgcgtgcgc ggtcgtcgac agggtcccca
     4381 gccccaccat cagcacaaag atcagggtcc atttcgtgcg tgaattgctc atgggtcctt
     4441 tccttgtagg ggggcggagt gcaggtttac agttaatgtt caactaaggg aagggaatcc
     4501 cctgcgagca tcacgcagaa cttcctcgcc acatggacgg cgagcatacg acatccgatt
     4561 accagcatcc cgtccacatc cccgtaccat gtgagcatcc gggttattta tcggcccgaa
     4621 accgcattcc tcctcgactt cgagaagctc cactcgttcc gagacgacgc cgacggcggc
     4681 gcagttgcct gtcggtcaag acagtccccg agcctacctc gcggcacctt ggggcatctc
     4741 cccggggtgt tccacagcct acagcgcgaa gccgaccgac acgtcgatca tgccggactg
     4801 ctggccgccg gccacggcgc gatccggcgc cggcagcggg gtgttgccgc gcgggtcgta
     4861 gctcgacatc gactgcagct gggcgccgtt gtacacgccg cccaggaggt acagcccgac
     4921 gccgagctcg acgtcgatgc gtccggcgat ccgcgcgccc gccgcggtgc gcaccgtgac
     4981 cagcccgccg ctctcgccgt cgacgtcgct gcgatcgagc cagcgcagcg tgccgcccgc
     5041 gaccaggtac ggcgtcaccg cgccgccgga cagcggcagc gcgagatcga gcgggtacat
     5101 ggcgaggccg aacgcgtgga gccgatccga cacgctcgcc agcccctcga gccggaaccg
     5161 cacgtggtcg ccgccgagct cgaccccggg cgcgaacgtg atcagcgtcg tgtgcagacc
     5221 gagcgcgctg gcgtcgatcg tcgcgctcgc catcgccgag ccgggcgcat agccgtgcag
     5281 ccggttgtac gccgccgatc ccgcgttgta gaccgccacc gcgtcggcga cgtggccgcc
     5341 gaccagcggc gtgtcctcgg acgggtccag cccgagcggc tcgacgccga gccggaacgt
     5401 ggcgcgcgca t
//
LOCUS       DASVOR010000478         8351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_384229,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000478 DASVOR010000000
VERSION     DASVOR010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8351
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384229"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /locus_tag="VF516_09510"
     CDS             <1..385
                     /locus_tag="VF516_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HEX8107952.1"
                     /translation="DARPAAQGPAAPDKPSSPFAQLEALRSSLPAGPAAPAQGAPAPD
                     ANPKGPARAVVRMERKGRGGKEVTVVDKLGLGPAALEAWCRDLKQALGCGGSVDGELI
                     VLQGDLRTRVPAVLTARGVGKVTVG"
     gene            640..2289
                     /locus_tag="VF516_09515"
     CDS             640..2289
                     /locus_tag="VF516_09515"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107953.1"
                     /translation="MRNLLSLSTGILFVSALGVAAGACASGPGQAKEPPVLHVSSPAR
                     SLVQGQAGSLTVTGTALPGPDGDPIAKVLVNGVEATLQPDGSFRAAIDLPQGETLIQT
                     VAKDAGGLTATDTRAVQAGQLMPVGSNVQRAVAAALSAGAFAKLSAAAGPIIKGINIP
                     ALIAPLQPMLSVGDSSTGAQLFVDNVKFSDVKISLTPVQDGLSFSAEIDQLDVPAHAS
                     FSVIGIGGSESLEVTADKIVVAGTLQVTPAGMSGFHTKLINPKVQLTNFMLHAGSVPD
                     AIIKLLNLQSAIQTIIAKVAELFMGPLVNQALGALAGPQKLPVLGHQLTMQVAPSAVH
                     FTPDGGLVEIDMKALLAGSESSPGFISTANGSPAMDPTFGFQLGLADDLANELLAELT
                     AVGALNLSVPQNVGVFDTVQIQMTLPPMINADASDGEMRLMLGDMFATFNSHGTPVGK
                     AAINAKVELKISPGTGGGSVALQLGTPELHVDTLDDIPNTTGLSGPDLANATAVGLGA
                     QIDAISKLLVAIPVPSIAGLTFRNLSLAGDEGYVVVSGQLQ"
     gene            complement(2301..3338)
                     /locus_tag="VF516_09520"
     CDS             complement(2301..3338)
                     /locus_tag="VF516_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107954.1"
                     /translation="MRGGLCPGILAGSALLGAGCDGVPLCRAPVFVAFAPTQITTDID
                     GFAPGVQTDVRLQTSLLVGDLVTLDVLSIDGTPLSTMVRGVDAQGEVEFDDVTVPTPQ
                     VVLRARGQDICGMGHDEITVDVSAGTRCALALDPAPEDRAYYAPLGVLNALTDLDPVT
                     PGYQATLRVASQPGWSIEVLGNGGATIGTVTAGSDGTASQPVTLPDGPAGFGAICRGG
                     GAQLLAHPVTVRVDTTPPSCALIGPAPGSLITSASDEDGDPRNGVQLTVIGSAPDLDV
                     AGEPVDVSLAETGIGAVTVPPTVTAADGTARAPVTLIPTGSPATYDVAITMHDHAGNA
                     CTAVTTYGVSL"
     gene            3508..4890
                     /locus_tag="VF516_09525"
     CDS             3508..4890
                     /locus_tag="VF516_09525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107955.1"
                     /translation="MKTFGFLLRLGSSLVGLSAVLAAGSPVRADTLGTSLPPFPAWID
                     PSDAVSPIALVQGGGIKVGEGTVLQPQVGLQTGVVSNVFYQNTNAVTAGLLRLLIEIG
                     TGSLPIQRLNPAVADDGTTPPAPGSPTPVLESGDFQYSANAYLSWDQYLSTNDNVTAQ
                     GGLGGGLVLRGLVNPQRPLQFAAQEVYSRLIRPVNFESQADANRDINNVSLRLNYVPT
                     GRSLSGYLYYQNTIDVFESSNQQFANRMLNQFGVHLDWAWLPLTVVSADLSGGYNTGI
                     GSSEKVTSTPLTATAGIGTALTLSTTISAHIGYANGFYASGPSYSSVQGGVLFGYRYS
                     PLGRVTAQYDYSQYDSINANFYSEHLVALTLEQYFASFILFARPELRLRSYQGTIVMD
                     VNGSTTRDDTIVAVTVGMRYNFRGWLAATLDYNLQDDQTDFRYTVGRFANLDPSYIRH
                     ELMLGVRAAY"
     gene            5013..7190
                     /locus_tag="VF516_09530"
     CDS             5013..7190
                     /locus_tag="VF516_09530"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX8107956.1"
                     /translation="MEAAAPEHEEEQVLAIDLRRYLAALRKYVWLLAALIVLSIAGAV
                     IFTTTQTPIYQATASVQVEPKLPDVLGTGDLLNVAGANAEYYKQQKQVLSSFTLIQKT
                     VEQNDLIPKLLSERERKGLSHDEQLQLATTRLQLDLAVKYPEADRIFYVSVKSPSAED
                     AKLIADAHVATYESYARGLLALSSNNASEALQAEFTDAEAKLRATEQKIYDFQAKNDM
                     VAVTIEAQQNLVQGNILAFTAKLNDARARQIELAARLAEMKKEQGEDVLASPVIMMGD
                     NPSFESLRTQYYTEKNRLIEIEKDLGPKHPDYIVQKQKVDLLYQALQGELKILVRGTQ
                     DQYNAQLAASAGLSAEIDKYKQEAKKLSPLIAIYNELLREKKEYDDKYNILRARLSSA
                     QMTGSLSSSVSNVRGLDKALLPQTPVSPNMRTNVTVAAVLALLAGIAIIFLIVFLDRS
                     IKTTGDATQAADAPVLGVIPQLTAAALGGASDDRARDMYVHENPKSPIAECCRSLRTN
                     ILFSAADRQLKTLVVCSANPREGKTTSVLYLGTTMAQSGQRTLVIDTDMRRPRLHRST
                     GVALAPGLSNLLIGEDSYDHLIKPTEVANLFILPCGLTPPNPAELLLTKRFEHVLGEL
                     ARRFDRVILDSPPIQAVTDAVVLSKRVDGVILVVRASKTMRDELRRSARQIRDVGGAI
                     VGVIVNELDARDYGYGGYGYGGYGYGRYYGPDPDPDAKPGEAA"
     gene            complement(7508..7834)
                     /locus_tag="VF516_09535"
     CDS             complement(7508..7834)
                     /locus_tag="VF516_09535"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HEX8107957.1"
                     /translation="MARATPRKPTRRWSKAVTEHSDALDLEPGVFKGSPAQIARSLQR
                     SAERSTRRKASPFRSALSMLVFYENRAGKNLPASRRRVLDQAKDELRKLYGRRPRRAA
                     VPRRAK"
     gene            complement(7836..8081)
                     /locus_tag="VF516_09540"
     CDS             complement(7836..8081)
                     /locus_tag="VF516_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107958.1"
                     /translation="MRENRITFPELALIAATRGMLGVGIGLLTAERLRAHTRHTVGWT
                     LFAIGALSTIPLVVRQVLRQRELSVQPGSRPPHTTAG"
     gene            complement(8133..>8351)
                     /locus_tag="VF516_09545"
     CDS             complement(8133..>8351)
                     /locus_tag="VF516_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107959.1"
                     /translation="DAERMAAFETISQAADSLRKLREESDQCIGEPITFSGGESSNSF
                     TGPQAPDDPTRGFGNHPIEPPAYASPFN"
BASE COUNT         1359 a         3063 c         2734 g         1195 t
ORIGIN      
        1 agacgctcgc cccgccgcgc agggtccggc ggcacccgac aagccgtcga gcccgttcgc
       61 gcaactcgag gcgctgcgca gctcgctgcc cgccgggccc gccgctccgg cgcagggcgc
      121 gcccgctccc gacgcgaacc cgaagggccc ggcgcgcgcg gtcgtccgca tggagcgcaa
      181 gggccgcggc ggcaaggagg tcaccgtcgt cgacaagctc ggtctcggtc cggcggccct
      241 cgaggcctgg tgccgcgatc tcaagcaggc gctcggctgc ggcggctcgg tcgacggcga
      301 gctgatcgtc ctgcagggcg acctgcgcac ccgcgtcccg gcggtgctga ccgcgcgcgg
      361 ggtcggcaag gtgaccgtcg gctgacgcac caacgaacgc gcacacaggg cgaagatctc
      421 ccactgcaaa cggtgaatcg catcggggcg tcgccatggc gactcgagtt gccaggcggg
      481 cggattgacc cgccgatcgg tggccgatcc agttgcaggt ccgccggttc gacgcagcgc
      541 gtcgactgta acccgcggtg cacaaaccga tctctccgaa gcacactcgg agtgtccgac
      601 gcgtcacgca tggcccttcg tttgctgtca gaggccgcga tgcggaacct cctctccctc
      661 tcgaccggga tcctcttcgt ctcggccctc ggtgtagccg ccggggcgtg cgcgagcggc
      721 cccggccagg ccaaggagcc tccggtcctc cacgtcagct cgccggcgcg gtcgctggtc
      781 cagggccagg ccggctcgct gaccgtgacc ggcaccgcgc tgcccggccc cgacggcgat
      841 ccgatcgcca aggtcctggt caacggggtc gaggccacgc tgcagccgga tggctcgttc
      901 cgcgccgcca tcgaccttcc gcagggcgag acgctgatcc agaccgtcgc caaggacgcg
      961 ggcggcttga ccgcgaccga cacccgcgcg gtccaggccg gccagctcat gccggtcggc
     1021 agcaacgtcc agcgcgcggt cgccgcggcg ctgtcggccg gcgcgttcgc caagctgtcg
     1081 gccgcggccg gcccgatcat caagggcatc aacatcccgg cgctgatcgc gccgctccag
     1141 ccgatgctca gcgtcggcga cagctcgacc ggcgcgcagc tgttcgtcga caacgtcaag
     1201 ttctccgacg tcaagatctc gctcacgccg gtccaggacg gcctgtcgtt cagcgccgag
     1261 atcgaccagc tcgacgtgcc cgcgcacgcg agcttctccg tgatcgggat cggtggctcg
     1321 gagtcgctgg aggtcaccgc cgacaagatc gtcgtcgccg gcacgctgca ggtgaccccg
     1381 gccgggatgt ccgggttcca caccaagctg atcaacccca aggtccagct caccaacttc
     1441 atgctccacg ccggctcggt ccccgacgcg atcatcaagc tgctcaacct gcagtcggcg
     1501 atccagacca tcatcgccaa ggtcgccgag ctgttcatgg gcccgctggt caaccaggcg
     1561 ctcggtgcgc tcgccgggcc gcagaagctg cccgtgctcg gccatcagct cacgatgcag
     1621 gtcgcgccgt cggcggtcca cttcacgccg gacggcggcc tggtcgagat cgacatgaag
     1681 gcgctgctcg ccggcagcga gtcgagcccc ggcttcatct ccaccgcgaa cggctcgccg
     1741 gcgatggacc cgacgttcgg cttccagctc ggcctggccg acgacctggc caacgagctg
     1801 ctcgccgagc tcaccgcggt cggggcgctc aacctgtcgg tgccgcagaa cgtcggcgtg
     1861 ttcgacaccg tgcagatcca gatgacgctg ccgccgatga tcaacgccga cgcgagcgac
     1921 ggcgagatga ggctgatgct cggagacatg ttcgcgacgt tcaacagcca cggcacgccg
     1981 gtcggcaagg cggcgatcaa cgccaaggtc gagctcaaga tctcgcccgg aaccggcggc
     2041 ggcagcgtcg cgctccagct cggcaccccc gagctccacg tcgacacgct cgacgacatc
     2101 cccaacacca ccgggctgtc cggcccggac ctcgcgaacg cgaccgcggt cggcctcggc
     2161 gcccagatcg atgcgatcag caagctgctg gtcgcgatcc cggtgccctc gatcgccggg
     2221 ctcacgttcc gcaacctgtc gctcgccggc gacgaaggct acgtggtggt gtccggccag
     2281 ctgcagtagc gcgggtccga ctagaggctc acgccatacg tcgtgacggc cgtgcacgcg
     2341 ttgccggcgt ggtcgtgcat cgtgatcgcg acgtcgtagg tcgccggcga gccggtgggg
     2401 atcagggtca ccggcgcgcg cgccgtgccg tcggcggcgg tgaccgtcgg gggcaccgtg
     2461 accgcgccga tcccggtctc ggcgaggctg acgtcgaccg gctcgcccgc gacgtcgaga
     2521 tccggcgcgc tgccgatcac ggtcagctgc acaccgttgc gggggtcacc gtcctcgtcg
     2581 gacgccgacg tgatcagcga gccgggcgcc ggcccgatca gcgcgcagct cggcggcgtg
     2641 gtgtcgaccc tcaccgtcac cgggtgcgcg agcagctggg cgccgccgcc gcgacagatc
     2701 gcgccgaacc cggccggccc gtcgggcagc gtgaccggct ggctcgccgt gccgtcgctg
     2761 cccgcggtga ccgtgccgat cgtggcccca ccgttgccga ggacctcgat gctccacccc
     2821 ggctggctcg cgacccgcag cgtcgcctgg tagcccggcg tcaccggatc cagatcggtc
     2881 agggcattga gcacgccgag cggcgcgtag tacgcgcggt cctcgggcgc gggatcgagc
     2941 gcgagcgcac agcgcgtccc ggccgacacg tcgaccgtga tctcgtcgtg acccatgccg
     3001 cagatgtcct gtccgcgggc gcgcagcacg acctgcggtg tcggaaccgt cacgtcgtcg
     3061 aactccacct cgccttgcgc gtcgaccccg cgcaccatcg tgctgagcgg cgttccatcg
     3121 atggagagca cgtccagcgt cacgaggtcg ccgaccagga gcgaggtctg cagccggaca
     3181 tcggtctgca caccgggcgc gaagccatcg atatcggtcg tgatctgcgt cggtgcaaac
     3241 gcgacgaaca ccggggcacg gcacagcggg acgccgtcgc agcccgcgcc cagcagcgca
     3301 gaaccagcga ggatcccggg gcaaagtcct ccccgcatcg cgcggcgatg ttactccgcg
     3361 cacggcccgc ccggaagccc gtgaacgcgg ccccccggca cggacactca cccccgtacc
     3421 gtccacacac cggcgtccca ccggcgtcgc cgccgcgaat gtcgtataga ttgcgcttcc
     3481 tcgcggtcga ccaggttagc gagcttcatg aaaacgtttg ggtttcttct tcgtctcggc
     3541 tcgagtctcg tcggactgag cgccgtgctc gccgcgggct cgccggtgcg cgcggacacg
     3601 ctcgggacct cgctcccgcc gttcccggcg tggatcgatc cgtccgacgc ggtgagcccg
     3661 atcgcgctgg tccagggcgg cgggatcaag gtcggcgagg gcacggtgct gcagccgcag
     3721 gtcgggctcc agaccggcgt ggtctcgaac gtgttctacc agaacaccaa cgcggtcacg
     3781 gcaggcctgc ttcgcctgct catcgagatc gggaccggat cgctgccgat ccagcggctc
     3841 aacccggcgg tcgccgatga cggcacgacc ccgcctgccc cgggcagccc gacgccggtg
     3901 ctcgagagcg gcgacttcca gtacagcgcg aacgcgtatc tgtcgtggga ccagtacctg
     3961 tcgaccaacg acaacgtcac cgcgcagggc ggcctcggcg gcggactggt gctgcgcggc
     4021 ctggtcaatc cccagcggcc gctgcagttc gcggcccagg aggtctacag ccggctgatc
     4081 cgcccggtca acttcgagtc gcaggccgac gccaaccgcg acatcaacaa cgtctcgctc
     4141 cggctgaact acgtcccgac cggccgctcg ctcagcggct acctgtacta ccagaacacg
     4201 atcgacgtgt tcgagtcgag caaccagcag ttcgccaacc ggatgctgaa ccagttcggc
     4261 gtgcacctcg actgggcgtg gctgccgctg accgtggtgt ccgccgacct ctcgggcggc
     4321 tacaacaccg gcatcggctc gagcgagaag gtcacctcca cgccgctcac cgccaccgcc
     4381 ggcatcggga ccgcgctcac gctcagcacc acgatcagcg cccacatcgg ctacgccaac
     4441 gggttctacg cctcggggcc gagctactcc agcgtccagg gcggcgtgct gttcggctac
     4501 cgctacagcc cgctcggccg ggtcaccgcg cagtacgact acagccagta cgactcgatc
     4561 aacgccaact tctacagcga gcacctggtg gcgctgacgc tcgaacagta cttcgcgtcg
     4621 ttcatcctgt tcgcacggcc cgagctgcgg ctccgcagct accagggcac gatcgtcatg
     4681 gacgtcaacg gcagcaccac gcgcgacgac acgatcgtcg ccgtcaccgt gggcatgcgc
     4741 tacaacttcc gcggctggct cgccgcgacg ctcgactaca acctgcagga cgaccagacc
     4801 gacttccgct acacggtcgg ccggttcgcc aacctcgacc cgagctacat ccgtcacgag
     4861 ctgatgctcg gggtccgcgc cgcgtactag cccgatggcg gattttcgcc gtacaatcct
     4921 cgtgttgcgc ggcccgcagg cgtgaacgcc ggcgcacgcc ccgacgacta agtcctgtct
     4981 gacccccgct cgtgcggagc aaaggccctg tcgtggaagc agcggcaccg gaacacgaag
     5041 aagaacaagt cctcgcgatc gacctccggc gctacctcgc cgcgctgcgg aagtacgtct
     5101 ggctgctcgc cgcgctgatc gtgctgtcga tcgccggcgc ggtgatcttc acgaccaccc
     5161 agaccccgat ctaccaggcg accgcatcgg tccaggtcga gcccaagctg cccgacgtgc
     5221 tcggcaccgg cgacctgctc aacgtcgccg gcgccaacgc cgagtactac aagcagcaga
     5281 agcaggtcct gtcgagcttc acgctgatcc agaagaccgt cgagcagaac gatctgatcc
     5341 cgaagctctt gtccgagcgc gagcgcaagg gcctgtcgca cgacgagcag ctccagctcg
     5401 cgaccacccg gctgcagctc gatctcgcgg tcaagtatcc cgaggccgac cggatcttct
     5461 acgtctcggt gaagagcccg agcgccgagg acgccaagct gatcgccgac gcccacgtcg
     5521 cgacctacga gagctacgcg cgcggcctgc tcgcgctcag cagcaacaac gcctcggagg
     5581 cgctgcaggc cgagttcacc gacgccgagg ccaagctgcg cgccaccgag cagaagatct
     5641 acgacttcca ggccaagaac gacatggtcg cggtcaccat cgaggcccag cagaacctgg
     5701 tccagggcaa catcctggcg ttcaccgcca agctcaacga tgcgcgcgcc cgccagatcg
     5761 agctcgccgc caggctcgcc gagatgaaga aggagcaggg cgaggacgtg ctggcgagcc
     5821 ctgtgatcat gatgggcgac aacccgtcgt tcgagtcgct gcgcacgcag tactacaccg
     5881 agaagaaccg cctgatcgag atcgagaagg acctcggccc caagcacccc gactacatcg
     5941 tccagaagca gaaggtcgac ctgctgtacc aggcgctgca gggcgagctc aagatcctgg
     6001 tgcgcggcac ccaggaccag tacaacgccc agctcgcggc cagcgccggg ctgtccgccg
     6061 agatcgacaa gtacaagcag gaggccaaga agctgtctcc gctgatcgcg atctacaacg
     6121 agctcttgcg cgagaagaag gagtacgacg acaagtacaa catcctgcgc gcgcggctgt
     6181 cgtcggcgca gatgaccggg agcctgagct ccagcgtgtc caacgtgcgc gggctcgaca
     6241 aggccctgct cccgcagacg ccggtgtcgc ccaacatgcg gaccaacgtc acggtcgccg
     6301 cggtgctcgc gctgttggcc ggcatcgcga tcatcttcct gatcgtcttc ctcgaccgct
     6361 cgatcaagac caccggcgac gcgacccagg ctgccgacgc gccggtgctc ggcgtgatcc
     6421 cgcagctgac cgcggccgcc ctcggcggcg ccagcgacga ccgcgcgcgc gacatgtacg
     6481 tccacgagaa ccccaagtcg ccgatcgccg agtgctgccg gtcgctgcgc accaacatcc
     6541 tgttctccgc cgccgaccgc cagctcaaga ccctggtcgt gtgcagcgcc aacccgcgcg
     6601 agggcaagac cacgagcgtg ctctacctcg gcaccacgat ggcgcagagc ggccagcgca
     6661 ccctggtgat cgacaccgac atgcgccggc cccggctcca ccgctcgacc ggcgtcgcgc
     6721 tcgcccccgg gctgtccaac ctcctgatcg gcgaggacag ctacgaccac ctgatcaagc
     6781 cgaccgaggt cgcgaacctg ttcatcctgc cgtgcggcct gaccccgccc aacccggccg
     6841 agctgctgct caccaagcgg ttcgagcacg tgctcggcga gctcgcccgc cggttcgacc
     6901 gcgtgatcct cgactcgccg ccgatccagg cggtgaccga cgcggtcgtg ctgtccaagc
     6961 gggtcgacgg cgtcatcctc gtggtccgcg ccagcaagac catgcgcgac gagctccgcc
     7021 gctcggcccg ccagatccgc gacgtcggcg gcgcgatcgt cggcgtgatc gtcaacgagc
     7081 tcgacgcgcg cgactacggc tacggcggct acggctacgg cggctacggc tacggccgct
     7141 actacggccc cgatcccgat cccgacgcca agcccggcga agccgcctga agaggtgtcg
     7201 atggaaggcc gagaacgtcc ttgcgaggac gttgaggtcg acctggacgt tgtcgcaaaa
     7261 cgtcgacctg aacggcgacg ttggacgttg actccatcct cgaccttgac cttgtacgga
     7321 aaccttctgg tggtctccga atcctcatcg aagaatcgcg accgatggtc aaagtggagg
     7381 tcgacgatgg actcgacggc cacggccacg tcaacctcaa cgacggccgt caacgtcgag
     7441 gtccaggtcg acgtctaccg gatgctggcg cacccgaagc aaggccccaa ccctttgatc
     7501 cggtgcgcta cttcgcgcgc cgaggcaccg cggcccgtcg tggccggcgg ccgtagagct
     7561 tgcgcagctc gtccttggcc tgatcgagca cgcgccggcg cgacgccggc aggttcttgc
     7621 ctgcgcggtt ctcgtagaac accagcatcg acagcgcgga gcggaacggg ctcgccttgc
     7681 ggcgcgtgct gcgctcggcc gagcgctgca gcgagcgagc gatctgcgcc gggctgccct
     7741 tgaacacgcc gggctcgagg tcgagcgcgt cgctgtgctc ggtcaccgcc ttcgaccacc
     7801 gccgcgtggg cttgcggggc gtggcgcgcg ccatgtcagc ccgcggtggt gtggggcgga
     7861 cgcgagccgg gctgtacgct cagctcgcgc tggcgcagga cctgacgcac gaccagcggg
     7921 atggtcgaca gcgcgccgat cgcgaacagc gtccagccca cggtgtggcg ggtgtgcgcg
     7981 cgcaggcgct cggcagtaag caatccgata ccgacgccga gcatgccccg ggtcgcagcg
     8041 atcaacgcaa gctcggggaa ggtgatgcgg ttctctcgca tggtggcctc ctgcgagagc
     8101 gacgtgcaga tcggatgccg gcagccgggc cgtcagttga acggcgacgc gtaggccggc
     8161 ggctcgatcg gatgattgcc gaagcccctg gtcggatcgt cgggcgcctg gggcccggtg
     8221 aacgagttcg acgactcgcc gccgctgaac gtgatcggct cgccgatgca ctgatcggac
     8281 tcctcgcgca gcttgcggag gctgtcggcg gcctgcgaga tcgtctcgaa cgccgccatc
     8341 cgctcggcgt c
//
LOCUS       DASVOR010000479         1905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501296,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000479 DASVOR010000000
VERSION     DASVOR010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501296"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            266..859
                     /locus_tag="VF516_09550"
     CDS             266..859
                     /locus_tag="VF516_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107960.1"
                     /translation="MSSFPRILYVPLFVLAGACGGGGSSAGGALSGTVHGQSIMIDDA
                     VSAAVTTDIVGVTIHGAAIVMANAKDLCSDAMSNTSHPNERGVFIVLTDINGMTFNTP
                     TAPGTYSIYQGTGTPPAKTAFFDVSATDATCKDVAAQDASATSGTVTLTSVSGNRFSG
                     NFDLVLDSGDHVTGSFDPSECPAVNTALNSTTAGSCM"
     gene            1023..>1905
                     /locus_tag="VF516_09555"
     CDS             1023..>1905
                     /locus_tag="VF516_09555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX8107961.1"
                     /translation="MTELTVEHKRLQEARSGVGWRTWGPYLSERQWGTVREDYSDNGD
                     AWAYFTHDQARSRAYRWGEDGLAGICDDQQRLCFALALWNERDPIVKERLFGLTNGEG
                     NHGEDVKEYYFYVDNLPTHSYQRWLYKYPQAAFPYDALRRENARRSRHEFEYELLDTG
                     VFASGRYFDVEVEYAKAAPLDVLCRITVYNRGDEAAPIHLLPTLWFRNTWSWAPHDPR
                     PRLSRIAGDHLIVRTEHHRLPPYHLHAEPGGELLFCDNETNARRLWGRETATRYVKDG
                     IGEHVVGGRIDTVNPAGE"
BASE COUNT          308 a          646 c          658 g          293 t
ORIGIN      
        1 gctcgtccgc gatctgcccg ccggcccgca cgccgtcacc gcctggctgc cgccgcgcgc
       61 cggacagccc gcgcggatcg ggcacggcac cgcgaccatc accgccggcg agctgaccga
      121 gctgacgatc gcgctggcgc cctcgccgtg acaccttccg gcagacccgg gagacggggc
      181 cgatgtggca gaacgtggca ggccggcccg ggcggcgatc gcgtaacact gccccgctgc
      241 cccgctccgt gcgataacgg cgcgcatgag ctcattccct cgcatcctct acgtcccgct
      301 gtttgtcctg gccggcgcct gtggtggagg cggttcctcc gcgggtggcg ccctcagcgg
      361 cacggtccac gggcagtcga tcatgatcga cgacgcggtc tcggcggcgg tgacgacgga
      421 catcgtgggt gtcacgatcc acggggcggc gatcgtgatg gccaatgcga aggacctgtg
      481 ctccgacgcc atgtcgaaca cctcgcaccc gaacgagcgg ggcgtgttca tcgtgttgac
      541 cgacatcaac gggatgacgt tcaacacgcc gaccgccccg gggacgtact cgatctacca
      601 gggcacgggg actccgccgg cgaagaccgc gttcttcgac gtctcggcga ccgatgcaac
      661 gtgcaaggac gtcgcggccc aggacgcgtc ggcgaccagc ggcacggtca cgctgacctc
      721 ggtcagcggc aaccggttct ccggcaactt cgatctcgtg ctcgacagcg gcgaccacgt
      781 gaccggcagc ttcgacccgt cggagtgtcc ggccgtcaat accgcgctca acagcacgac
      841 ggccgggtcg tgcatgtgac ccacgagatc ggcgcaggca tcggcctcgc cggagcgttc
      901 tgagcagcgc tgcacgactg gtcgagcatc ggcgcacgac gacgatcgcg ggcgtcgcgg
      961 catggggctt gctgccccgg tgagtccgtc tggctcggat gagccatgcg gaggaaccgg
     1021 tcatgacaga gctgactgtc gagcacaagc ggctgcaaga ggcgcgttcg ggtgtcggct
     1081 ggcgcacgtg ggggccttat ctgagcgagc ggcaatgggg cacggtgcgc gaggactaca
     1141 gcgacaacgg cgacgcgtgg gcgtacttca cgcatgatca ggcgcgctcg cgcgcctatc
     1201 gctggggcga ggacgggctc gcggggatct gcgatgatca gcagcgcctg tgcttcgcgc
     1261 tcgcgctgtg gaacgagcgc gatccgatcg tcaaggagcg cctgttcggg ctcaccaacg
     1321 gagagggcaa tcacggcgag gacgtcaagg agtattactt ctacgtcgat aaccttccga
     1381 cgcattccta tcagcgctgg ctctacaagt acccgcaggc ggcgtttccc tacgatgccc
     1441 tgcggcgaga gaatgcccgt cggtcgcgcc acgagttcga gtacgagctg ctcgataccg
     1501 gcgtgttcgc cagtggccgg tacttcgatg tcgaggtcga gtacgccaag gccgcgccgc
     1561 tcgacgtgct gtgtcggatc accgtgtaca accgcgggga tgaggctgcg ccgatccacc
     1621 tgttgccgac gctgtggttc cgcaacacct ggtcgtgggc gccgcacgac ccgcgccctc
     1681 gtctgtcgcg gatcgcaggc gatcatctca tcgtccgcac cgagcaccac cgcctgccgc
     1741 cgtaccatct gcacgccgaa cccggcggcg agctgctgtt ctgcgacaac gagaccaacg
     1801 cgcggcggct gtgggggcgc gagaccgcga cgcgctacgt gaaggatggg atcggcgagc
     1861 atgtggtcgg cggccggatc gacacggtca accccgccgg cgagg
//
LOCUS       DASVOR010000480         6071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_325905,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000480 DASVOR010000000
VERSION     DASVOR010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325905"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1827)
                     /locus_tag="VF516_09560"
     CDS             complement(<1..1827)
                     /locus_tag="VF516_09560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107962.1"
                     /translation="MRRSLPVVFALVWLGSGAARADDKYRPAPAAIAEVLHAPASPTI
                     RISPDGRYALFMRPQLYPRIADLAAPMLRVAGVRINPDNNGLHSWPHYQGGITIKPLP
                     DGKDAAIRMPADLAFSDPVWSADATAFAFLAIGKTAIQLWLVDVKTAQARMVPGVAIN
                     PVLEAGIVAGNALNRSLGQSVQWMPDGRSLLVKAVPVGRKPAPAEGPPIGPIVEDATG
                     IARASSTYEARDLLRTPHDADLFEYYATSQLAVVDARTLDVRRFGPAGVLSEVRIAPG
                     GKVALVSRIKRPYSFSRPWRRFAHDVEVWSLDGNVVESVARLPLADQVPIDGEPTGPR
                     DVAFVPTSPATLFWVEALDEGDWAVKAAHRDRLVLKEVGKPAATWFELEQRFRSVDWI
                     DDGATALVTEWQRDRRWLRTFVARLTERSGGLRKLWDRSEHDIYGAPGAPRQHQLPSN
                     RFAVQSDGTSIWLTGDGSSQAGDRPFLDRLDLATLRTERVFRADRDSYERVDHVYAGD
                     TRSFLTIKESPKDPPNLALRTLGAAVAAPAAGEASVASDLKMLTAVPDPTPQIRGVAK
                     QVVTTKRADGTQVSFTLYLPPGHRPGTRLPTVFWAYPREFSDP"
     gene            complement(1983..2492)
                     /locus_tag="VF516_09565"
     CDS             complement(1983..2492)
                     /locus_tag="VF516_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107963.1"
                     /translation="MEASLTVASAMAVLLVSGPGCDHRGGPPMKPVEETKTLSATLEL
                     LPSGNDWSIGFTLNNPTAQAIDATIHEPFASFTVEVATDDGKRLELVQPALDVPVHPV
                     PFHLGAGETMRLATPFRLRFDPKVPPAGGDDPFLWSIASVRVSVVISARLTIEGLAPI
                     AAQAHLHPG"
     gene            complement(2477..2992)
                     /locus_tag="VF516_09570"
     CDS             complement(2477..2992)
                     /locus_tag="VF516_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107964.1"
                     /translation="MDTVNWTFGPKVNVPTTINQIEGDSEEDLGKIYNYKVGPALGKD
                     GKDDYLHQTILETFGTRTSNLKVADLKPDWAKSHGTTTDQAITDYFFGTRSDNGTFTI
                     SAGDKFQDQHGGGVPDKARFEEALTTMKDVTCDLPQTYSATPGTALGNFTVRRILKLN
                     GDKKVKKWKRP"
     gene            3691..5001
                     /locus_tag="VF516_09575"
     CDS             3691..5001
                     /locus_tag="VF516_09575"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8107965.1"
                     /translation="MPRIFLSYATPDRERALAVKVLLEIGGGEVFMDCYKIPYGSDWR
                     AVIEQAVNECDVMCVFWSRHTPRSMWVEREYSTFMRGHPERPCVPLCADETPLPGALA
                     ARQAPPFLELTNQFLALRGRLRRQGVSRRETKRLVELELEASGVRINGRQKKQLLKLF
                     VGGISIFALLTWFFEIVPKWKLALAAGLCVAGAFAAHETCTAGDQTVPTATPARPAAA
                     TPVPTPASTSIPVAAEIDASLPLDTSHEADAHDPDCLRREPYIVRPKEVKPVVEHTED
                     PLCALLAKLYPASDRGFVGIAVEPGVAYRSDSDRTTPAEFPICDDAVQYVHWASSIGF
                     EPFQCDIDMRLIWRPRTAVTASVVFKCSKLVYAAHRSAELARLRDSFERCLTRSDGWE
                     REAPRDPEAIGADFSRDRWRTTLRTEDIGNGAQYRLSFDVRSMR"
     gene            5074..5526
                     /locus_tag="VF516_09580"
     CDS             5074..5526
                     /locus_tag="VF516_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107966.1"
                     /translation="MSGRLVAPSTAPLTAAPGGDEIYVTSQLFACTTPVRVNLQEPSA
                     LVVERFVRELGLPRQVAHGTALGFHISYALARDDQRLSSARSLADQGVGPGQLLWLET
                     DLRPLPGPSRSRAAWAMQRVAASTPMGTPRLAGSCWRRSSAGLTRPGR"
     gene            complement(5547..5846)
                     /locus_tag="VF516_09585"
     CDS             complement(5547..5846)
                     /locus_tag="VF516_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107967.1"
                     /translation="MPGIRETLVRIGALVAGIALASCHAGDNIRGDAHCPVECAVDCP
                     PDVACPDYAICAAPPRYICPPGYKQIGSSSRLDPPRAWPSTNTSADTAGASTAHR"
BASE COUNT          893 a         2067 c         2134 g          977 t
ORIGIN      
        1 cggatcggag aactcgcgcg gataggccca gaacaccgtc ggcagccgcg tgcccggccg
       61 gtggcccggc ggcaggtaca gcgtgaacga gacctgggtg ccgtcggcgc gcttggtcgt
      121 caccacctgc ttggccaccc cgcggatctg cggcgtcggg tcggggaccg cggtcagcat
      181 cttcaggtcg ctcgccacgc tcgcctcgcc ggcggcgggc gcggcgaccg cggcgccgag
      241 cgtgcgcagc gcgaggttgg gcgggtcctt cggcgactcc ttgatggtca ggaagctgcg
      301 cgtgtcgccc gcgtacacgt gatcgacgcg ctcgtagctg tcgcgatcgg cgcggaacac
      361 ccgctcggtg cgcagcgtgg cgaggtcgag gcgatcgagg aacgggcggt cgccggcctg
      421 gctcgagcca tcgccggtca gccagatcga cgtcccatcg ctctgcacgg cgaaccggtt
      481 gctgggcagc tgatgctgcc gcggcgctcc gggcgcgcca tagatgtcgt gctcgctgcg
      541 gtcccacagc ttgcggaggc cgccggagcg ctcggtgagc ctggcgacga acgtccgcag
      601 ccagcggcga tcgcgctgcc actcggtgac cagcgcggtc gcgccgtcgt cgatccagtc
      661 gaccgaccgg aagcgctgct cgagctcgaa ccaggtcgcc gccggcttgc cgacttcctt
      721 gagcaccagc cgatcgcggt gcgccgcctt gaccgcccag tcgccctcgt cgagcgcctc
      781 gacccagaac agcgtcgccg gcgacgtggg cacgaacgcg acatcgcgcg gcccggtcgg
      841 ctcgccgtcg atcgggacct ggtcggcgag cggcaggcgc gcgacgctct cgacgacgtt
      901 gccgtcgagg ctccacacct cgacgtcgtg cgcgaaccgc cgccacggcc gcgagaacga
      961 gtacgggcgc ttgatccgcg acaccagcgc caccttgccg cccggggcga tccgcacctc
     1021 gctcagcaca ccggcggggc cgaaccggcg gacgtcgagc gtccgggcat cgacgacggc
     1081 gagctgggac gtcgcgtagt actcgaacag atcggcgtcg tggggggtgc gcagcaggtc
     1141 gcgcgcctcg tacgtgctgc tcgcccgcgc gatgccggtc gcgtcctcga cgatcggacc
     1201 gatcggcggc ccctcggcgg gtgccggctt gcgccccacc gggacggcct tgaccagcag
     1261 ggaccggccg tcgggcatcc actggaccga ctggccgagc gagcggttga gcgcgttgcc
     1321 ggccacgatg cccgcctcga gcaccggatt gatcgccacg ccggggacca tcctcgcctg
     1381 cgcggtcttg acgtcgacca gccacagctg gatcgcggtc ttgccgatcg ccaggaacgc
     1441 gaacgccgtc gcgtccgcgc tccacaccgg atcgctgaac gccagatccg cgggcatgcg
     1501 aatcgccgcg tccttgccgt cggggagcgg cttgatcgtg atgccgccct ggtaatgcgg
     1561 ccacgaatgc aatccgttgt tgtccggatt gatccgcacg cccgcgacgc gcagcatcgg
     1621 cgccgccaga tccgcgatcc gcgggtagag ctgcgggcgc atgaacagcg catagcggcc
     1681 gtcggggctg atccggatcg tcgggctcgc cggggcatgc aacacctcgg cgatcgctgc
     1741 aggagcgggt cggtacttgt cgtcggcgcg agcggcaccg gagcccagcc acacgagcgc
     1801 gaacaccaca gggagggagc gacgcatggc gcgcattgta gcggcgcggg cgaccgtgat
     1861 cgctgcaacc gagggccgcc atcaccgagc gatcgaggct gccggagcgc tcctcgtgat
     1921 cggtggtcag accaatcgcc gagcggcgcc tcaccgcgga cgccgagcga ccccgtgggc
     1981 gatcagccgg ggtgcaggtg agcctgtgca gcgatcggag ccagaccctc gatcgtcagg
     2041 cgagcggaga tcacgacaga cacgcgcacg ctggcgatcg accagaggaa gggatcgtcg
     2101 ccgccggccg ggggcacctt ggggtcgaac cgcaagcgga atggagtggc gaggcgcatg
     2161 gtctcgccgg cgccgagatg gaatggcact ggatgcaccg gaacgtcgag cgcgggctgc
     2221 accagctcga gccgcttccc gtcgtcggtg gcgacctcga ccgtgaacga cgcgaacggc
     2281 tcatggatcg tcgcatcgat cgcctgtgcg gtcgggttgt tcagcgtgaa tccgatcgac
     2341 cagtcgttcc ccgacggcaa cagctcgagc gtcgccgaca gtgtcttggt ttcttccact
     2401 ggtttcatcg gcggacctcc gcgatggtcg caccccggac cggacacgag cagcacggcc
     2461 atcgcgctcg cgaccgtcag ggacgcttcc atttcttgac cttcttgtcg ccgttgagct
     2521 tgaggatccg gcgcaccgtg aagtttccga gcgctgttcc cggggtcgcg gaatacgtct
     2581 gcgggagatc gcacgtgacg tccttcatcg tcgtcagcgc ttcctcgaac cgggccttgt
     2641 cgggaacgcc accgccgtgc tgatcctgga acttgtcgcc ggcgctgatc gtgaacgtgc
     2701 cgttgtcgct gcgtgtgccg aagaagtagt ccgtgatcgc ctgatcggtc gtggtgccgt
     2761 ggctcttggc ccagtccggc ttgagatcgg cgaccttgag gttcgacgtc ctggttccga
     2821 acgtctcgag gatcgtctgg tgaaggtagt cgtccttccc gtccttcccg agcgccggac
     2881 cgaccttgta gttgtagatc ttgccaagat cctcctcgga atctccttcg atctggttga
     2941 tcgtcgtcgg gacgttgacc ttggggccga aggtccagtt cacggtatcc accaccgact
     3001 tgtccgcgac cttctgcagc tcgagctggg cgcgtcgaag tcgccgccgg cgcaggtgaa
     3061 cacgagctgg tactgctcgg ggttggagcc ggtgtagacg gcgccgaaga tgatccgttc
     3121 gccgggggcg gacagtggcg cgcgatcatg cgggatgatg ttgtaccgcg ccggcggctc
     3181 atccgacgat ggcgcggtcg cacttccggc ggagtctgcc tgcccggcac ggtcgtcgct
     3241 cggcgcccgc gtggctgggg cagcgggcgc cggcacctgg ctcacggcgc cgatggccgg
     3301 gcgggcttga tcgggttcgc cattgcgctc gcggggcccg ccgtacacct cgtccacgag
     3361 cgttgtcttg cctgggcttg ctacgcggga cccgccgcct gcatgagcac ccccggtcgt
     3421 ccgctcccga gacgtccaca tggccatcgt acggtaaggg cgccccaggc cttccttgtg
     3481 gccaagtcgg aagccccggg ccgctgaggc gccggaattc gttcccggaa ccgctgctcg
     3541 tgcccacggc accgcgtcag caacgcgttg gcgtccagcc caaggccggg cgtcggcgac
     3601 gtcggcgatc gtacaagcag aacgccgacg cagtcggccg atcgtgtggt gccatcgcgg
     3661 cggcgatgcg tgtatcgtgt gatcggaggg atgcctcgga tcttcctgtc ctatgcgacg
     3721 ccggaccgcg aacgcgcgct ggccgtgaag gtcctgctcg agatcggcgg cggcgaggtg
     3781 ttcatggact gctacaagat cccgtacggg tcggactggc gcgcggtgat cgagcaagcg
     3841 gtgaacgagt gcgacgtgat gtgtgtgttc tggtcccgtc acacgccgcg ctcgatgtgg
     3901 gtggagaggg agtacagcac gttcatgcgg ggacatcccg agcgcccttg cgtcccactg
     3961 tgcgccgacg agaccccgct gcccggcgcg cttgcggctc ggcaggcacc tccgtttctc
     4021 gagctcacca atcagtttct cgccctgagg gggcgactgc gccgccaggg cgtgagccgc
     4081 cgcgagacca agcggctcgt cgagctcgag ctggaagcct cgggtgtacg aatcaacggc
     4141 cgccagaaga agcagctgct gaagctcttc gtcggcggga tctcgatctt cgcgttgctc
     4201 acgtggttct tcgagatcgt gccgaaatgg aagctcgcgc tcgccgccgg tctgtgcgtg
     4261 gcaggcgcat tcgcggctca cgagacctgc accgccggcg atcagacggt gccaaccgcc
     4321 acgccggccc gccccgcggc ggccacgccg gtcccgacgc ctgcatcgac gtcgatacca
     4381 gtggccgccg agatcgacgc ctccttgccc ctcgatacga gccacgaggc cgatgcgcac
     4441 gatcccgact gcctccggcg cgagccgtac atcgtgcgcc caaaggaggt caaacccgtc
     4501 gtcgagcaca ccgaggaccc gttgtgcgcg ctgctcgcaa agctgtaccc ggcgagcgat
     4561 cgcggattcg tggggattgc agtggagccg ggagtcgcgt acaggtcgga cagtgatcgg
     4621 acaacaccgg cagagttccc gatctgcgac gatgcggtgc aatatgttca ctgggcctcg
     4681 tcgatcggct tcgagccatt ccagtgcgat atcgacatgc gactgatctg gaggccccgc
     4741 accgcggtga cggcctcggt tgtcttcaag tgctcgaagc tcgtctatgc ggcccatcgc
     4801 agcgcagagc tcgcgcgttt gcgcgacagc ttcgagcgct gcctgaccag gagcgacggt
     4861 tgggaacggg aggccccgag ggaccccgag gcgattggcg cagacttctc gcgagatcgg
     4921 tggcggacga cgctccgtac cgaggacatc ggtaacgggg cacagtatcg gctgagcttc
     4981 gatgtccgga gcatgcgatg agcgagcccg cctgccgtgg tccaggcaga gaggatcctc
     5041 gacagcttcg gccgcgaccg cgcggcggcg cagatgtctg gccgcctggt ggcgccttcg
     5101 accgcaccgc tgaccgccgc gcccggtggc gacgagatct atgtcacctc gcagctgttc
     5161 gcctgcacca cgcccgtgcg cgtgaacctg caagagccca gcgccctggt ggtggagcgc
     5221 ttcgtccgcg agctgggatt gccccggcag gtggcgcatg gcacggcgct cggctttcac
     5281 atcagctacg cgcttgcgcg cgacgaccag cgcctcagct ccgcgcggtc gctcgccgat
     5341 cagggtgtcg gtcccggaca gctgctctgg ctcgagaccg atctccgtcc gttgccgggg
     5401 ccgagccggt cgcgggcagc ctgggccatg caacgtgtcg ccgcctcgac accgatgggg
     5461 acgccgaggc tcgccggcag ctgctggcgg cggtccagcg ccggcctgac ccgtccggga
     5521 cgctgacgtg cgtgcgagct atcccgtcac cggtgtgcag tgctcgcgcc cgcggtgtcc
     5581 gcgctcgtgt tcgtcgacgg ccatgcccgt ggggggtcga ggcgcgacga gctcccgatc
     5641 tgcttgtagc ccggcggaca gatgtacctg ggcggagccg cgcagatcgc atagtcggga
     5701 cacgcgacgt ccggaggaca gtcgaccgcg cactcgacgg ggcagtgtgc atcgccccgg
     5761 atgttgtcgc cggcgtggca gctcgcgagg gcgatgccgg cgacgagcgc accgatccgg
     5821 accaaggttt cgcgaatacc aggcatgggt ggtgcctgga atctctttag cacgccatgc
     5881 cgagcgggag catcccttca gcggtaaggt cgggcgtgtc ctcgatcgcg atccgggcgc
     5941 gctcgctgcc gcggcggcgg ttcacgcgcg cgttgctcga cgacctgcac gggctggcca
     6001 accggctgct cgccgaggac gccgcgcact tccgggtcca cgccgagacc aacgacgtgg
     6061 tccacatcta c
//
LOCUS       DASVOR010000481         5040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_267243,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000481 DASVOR010000000
VERSION     DASVOR010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267243"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            701..4219
                     /locus_tag="VF516_09590"
     CDS             701..4219
                     /locus_tag="VF516_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8107968.1"
                     /translation="MTDGVAPVQQSAQAVSGPARGPANALPYATDRILVRFKSGTSAS
                     RTSAVHAQHSAQVVREYRVPTNLQLVTVPQGATAEQMVEAYRSDPEVLYAEPNYIYQL
                     ATVPNDPSFSNLWGMNNTGQLGGIVDADINAPEAWNITTGSSSVILGLLDTGVDYNHQ
                     DLAANIFTNAGEIPGNGIDDDGNGWIDDVHGIDAIAESGDPMDTDGHGTHVSGTMAAR
                     GNDATGVAGVNWNAKIVACKAFGPFGGQLSDILQCMDYFLALKTRPNNPVDIVATNNS
                     WGGGPFSQALLDAIAAHRQAGMLFVAAAGNNGSNNDQIPFYPATYELDNIISVLAFNR
                     FNQRSFFSNFGALTVDVGAPGEDVFSTLPGNSYGTLSGTSMATPHVTGLVGLLKAQNP
                     GRSAQQIKNLILTGGTASPATTGATLSGRRIRADGSLSCTNQSLVNRFAPATSSVVVG
                     IGTPVPLGMLSITCDAPSPGPQVVTIVETGATIALVDGNGSGQFAGTYTPVSPGTTTL
                     AFPNGDTVTVTAIGNYDPAQVVPFEFPAITGTVLPLACDLCFTTLQLPFPIQFGGTSP
                     GLTSLNIGSKGVLTFSAQSSFGFDNQPLPTSAASTIVAPFWDDLFPAVGGSVRFAVLG
                     QAPSRQLVIEYRDVPHFAGFGAVKFQVVFFEGSPNIRFNYADVSFDSPPFDNGASQTV
                     GVQVAPGIARQFSFNTPSLSNSMSLLWTIGAPLAVAGPDQVVLPGTAVALDGTASHDF
                     DGTIASYAWTQTAGTPVTLTGANTATPSFTAPAASGTLTFQLTVTDNDGKTGTDSVDV
                     VVNQAPVAAAGDDLTIPTDLPATLDGTGSFDPDGVITGYQWTQIQGAPVQIQNAGTAV
                     ATFIAPATAGVLVFRLTVTDQFGFTNSDTVTANVFLNLKPVARAGQDRIVRPGTSVTL
                     DGTASNDPDGTIASYAWTVTTCLTVTGPCDLKLSGAATATPQFQAPTSPGIIALQLTV
                     TDNVGAVATDTVEISVFLQAPTAAIVAATSCVQRGSTVTLNGTGSSDADGTIVAYAWT
                     QLSGPPVTLSGAGTPIASFTAPTQGTLVFALTVTDSDGLTGTAQVTIPIAPLPVADAT
                     ASDAVVSQGATVTLSGSQSVGAVLYSWKQIAGARASLSNASAVSPTFVAPRPTGQFDI
                     LTFELTVTDVCGITATDTISLTVLRK"
     gene            complement(4242..>5040)
                     /locus_tag="VF516_09595"
     CDS             complement(4242..>5040)
                     /locus_tag="VF516_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107969.1"
                     /translation="TVQFDVTPAPGAAAGEARPVVRIGDAAYSLREDVIDHPHIPVQV
                     VLRPATVALVPLSIKLPAGRIAYVKGSGDSIAADLAHVGLTVDELDDDALRTGDLSRY
                     TAVVLGIRAYNTRPAVRAAHAHLMSYVERGGTVVVQYNTLDFEGPIGPFPLELGRDRI
                     TDENAAVTFLDPRQPVLHHPNEITAADFTGWVQERGIYFGSKWDARYTPILRFADPGE
                     GPLDGSLLVAHHGKGRYVYTGLVFFRELPAGVPGAYRLFANLLAAGK"
BASE COUNT          825 a         1813 c         1660 g          742 t
ORIGIN      
        1 gccggccgcc ccacgcggcg agggcggatc ggcggcgatg gccgtggcga ccgctcgatg
       61 ctgctcgagg caggagcagg tgcagcgcgg ccgcctcccc acgggtcagc atggttgccg
      121 tccgccctct ggacaccgtg catcgcacgt cggtggcggc accgcagcga gcgacggtgg
      181 cccgagaatc acaccgccag ctcgggccgt cgaatgctgg atgctacgtg aacaaggcgt
      241 gatcgagacg gggacaggtc atcgtgccgg atagtgagtc acgacctcgg gcgaccgtcg
      301 tccaggactc aggaatccgc agagtcatcg gtagcgattc gacacgagcc gcaggccacg
      361 ccacccgcaa gcgggcagca taacaaggcc cccctgacag gaatggaagt ccggagggag
      421 cgcttccatc gaacgctctt tgccacattg catctgtagc ctttcgggtt atgtgacgcc
      481 tggagttttg gaatctctct gtgggtgact gcgcatcgga cggcgggtcc ggagtggcag
      541 ctgcgggcag attttgatca ctcagtacgc ctgcactgta attgtaacac gtactcatca
      601 tgaggagctc acaacgatga tcaagcaagt cagagggagt ggatggactg cggtggcagg
      661 aggagcgatc gcgctgctcg ctgccgcatc atgcgccgat gtgaccgacg gcgtggcgcc
      721 cgtgcagcag agcgcccagg ccgtctccgg cccggcccgc ggtccggcca acgcgctgcc
      781 gtacgcgacc gaccggatcc tcgtgcgctt caagagcggc acgtccgcca gccgcacgtc
      841 cgccgtgcac gcccagcatt ccgcgcaagt cgtccgcgag taccgcgttc cgacgaacct
      901 gcagctcgtc acggtgccgc agggcgcgac agcggagcag atggtcgagg catatcgcag
      961 cgatccggag gtgctctacg ccgagcccaa ctacatctat cagctcgcca cggtgcccaa
     1021 cgaccccagc ttcagcaacc tctggggcat gaacaacacc ggtcagctcg gcggcatcgt
     1081 cgatgccgac atcaacgcgc ccgaggcctg gaacatcacc acgggtagca gcagcgtcat
     1141 cctcggcctg ctggacaccg gcgtggacta caaccaccag gacctggccg ccaacatctt
     1201 caccaacgcc ggcgagatcc ccggcaacgg tatcgacgac gacggcaacg ggtggatcga
     1261 cgacgtccac ggcatcgacg ccatcgccga gtcgggcgat ccgatggaca ccgacggcca
     1321 cggtacccac gtcagcggga cgatggccgc gcgcggcaac gacgccaccg gcgtggccgg
     1381 cgtgaactgg aacgcgaaga tcgtcgcctg caaggccttc ggcccgttcg gcggccagct
     1441 cagcgacatc ctgcagtgca tggattactt cctcgcgctg aagacccggc cgaacaaccc
     1501 ggtcgacatc gtcgccacca acaactcgtg gggtggcggg ccgttctcgc aggcgctgct
     1561 cgatgcgatc gccgcccatc gccaggccgg catgttgttt gtcgccgccg ccggtaacaa
     1621 cggctcgaac aacgaccaga tcccgttcta tccggcaact tatgagctcg acaacatcat
     1681 ctcggtgctc gcgttcaatc gcttcaacca gcgctccttc ttcagtaact tcggcgcgct
     1741 cacggtggac gtgggcgccc ctggcgagga cgtgttcagc acgctccctg gcaacagcta
     1801 tgggaccctc agcgggacgt ccatggcgac gccgcacgtg accgggctcg tcggtctgct
     1861 caaggcgcag aatcccggcc gcagcgcgca acagatcaag aacctgatcc tgaccggcgg
     1921 caccgcctcg ccggccacga ccggcgccac gctgtccgga cgccggatcc gcgccgacgg
     1981 ctcgctgagc tgcacgaacc agtccctggt gaaccggttc gcccccgcga cctcttcggt
     2041 cgtcgtcggt atcggcacgc cggtcccgct gggcatgctc agcatcacct gcgacgcgcc
     2101 cagcccggga ccgcaggtcg tgaccatcgt cgagaccggc gccaccatcg cgctggtcga
     2161 cgggaatggc agcggtcagt tcgcgggaac ctacacgccg gtgagccccg gcacgacgac
     2221 gctggccttc cccaacggtg acaccgtcac cgtcactgcc atcggaaact acgatccggc
     2281 acaggtcgtg ccgttcgagt tcccggcgat caccggcacc gtgctgccgc tggcctgcga
     2341 cctctgcttc acgaccctcc agctgccgtt cccgatccag ttcggcggca cctcgcccgg
     2401 cctgacgtcg ctgaacatcg gcagcaaggg cgtcctcacc ttcagcgcgc agagctcgtt
     2461 cggtttcgac aaccagccgc tgccgacgtc cgctgccagc acgatcgtcg cgccgttctg
     2521 ggacgatctg tttccggccg tcggcggatc ggtccggttc gcggtcctgg gccaggcgcc
     2581 gagccggcag ctcgtgatcg agtaccgcga cgtgccccat tttgcgggct ttggcgcggt
     2641 caagtttcag gtcgtgttct tcgagggcag cccgaacatc cgcttcaact acgccgacgt
     2701 gtcgttcgac agccccccgt tcgacaacgg cgcttcgcag acggtcggcg tgcaggtggc
     2761 gcccggcatc gcccggcagt tcagcttcaa cacgcccagc ctctcgaaca gcatgtcgct
     2821 gctctggacg atcggcgcgc ccctggccgt ggccggcccc gatcaggtcg tcctgcccgg
     2881 cacggcggtc gcgctcgacg gcacggccag ccacgacttc gatggcacga tcgcctcgta
     2941 cgcctggacc cagaccgcgg gcacgccggt cacgctgacc ggcgcgaaca ccgcgacgcc
     3001 gagcttcacc gcgccggcgg cctcgggcac gctgaccttc cagctcacgg tcaccgacaa
     3061 cgacggcaag accggcaccg actcggtcga cgtggtcgtc aaccaggccc cggtcgccgc
     3121 ggccggcgat gacctcacga tcccgaccga ccttccggcc acgctcgacg ggaccggcag
     3181 cttcgatccc gacggcgtga tcaccggcta ccagtggacg cagatccagg gcgcgccggt
     3241 gcagatccag aacgccggta cggcggtggc cacgttcatc gcgccggcca cggccggcgt
     3301 cctcgtgttc cggctcaccg tcaccgacca gttcggcttc acgaacagcg acaccgtgac
     3361 ggccaacgtg ttcctcaacc tcaagccggt ggcccgtgcg ggccaggatc ggatcgtccg
     3421 cccgggcacc tcggtgaccc tcgacggcac cgccagcaac gatccggacg gcaccatcgc
     3481 gtcctatgcg tggaccgtca cgacgtgctt gaccgtcacc ggcccgtgcg acctcaagct
     3541 cagcggtgcc gccacggcga ccccgcagtt ccaggcgccg acctcgcccg gcatcatcgc
     3601 cctccagttg acggtgaccg acaacgtggg tgcggtcgcg accgacaccg tcgagatcag
     3661 cgtgttcctc caggcgccga ccgcggcgat cgtggcggcc acctcgtgcg tgcagcgcgg
     3721 cagcaccgtc acgctcaacg gcacgggcag cagcgacgcc gacggcacca tcgtcgccta
     3781 tgcgtggacc cagctgtccg gtccgcctgt gaccctgagc ggcgcgggta ccccgatcgc
     3841 ctcgttcaca gcaccgaccc agggcacgct ggtgttcgcg ctgaccgtca ccgacagcga
     3901 cgggctcacc ggcaccgccc aggtcacgat cccgatcgcg ccgctgccgg tcgccgacgc
     3961 caccgcgagt gacgcggtcg tgagccaagg cgcgaccgtg acgctcagcg gctcgcagag
     4021 cgtcggcgcg gtcctttact cctggaagca gatcgccggc gccagggcga gcctcagcaa
     4081 cgcgagcgcg gtctcaccga ccttcgtggc gccccggccg acgggccagt ttgacatcct
     4141 gacgttcgag ctcacggtca ccgacgtctg cggcatcacg gcgaccgaca cgatctcgct
     4201 caccgtgctc cgaaagtgag cggacagggc ggggcgcgac gtcatttgcc ggccgcgagc
     4261 aggttggcga acagccggta ggcgccgggc acgccggcgg gcagctcgcg gaagaacacc
     4321 aggccggtgt agacgtagcg gcccttgccg tggtgcgcga ccaggaggct gccgtcgagc
     4381 gggccctctc cggggtcggc gaaccgcagg atcggcgtgt agcgcgcgtc ccacttggac
     4441 ccgaagtaga tcccgcgctc ctggacccag ccggtgaagt cggcggcggt gatctcgttg
     4501 gggtggtgga gcaccggctg cctgggatcg aggaacgtca cggcggcgtt ctcgtcggtg
     4561 atccggtcgc ggccgagctc gagcgggaat gggccgatcg ggccctcgaa gtcgagcgtg
     4621 ttgtactgca cgaccaccgt gccgccgcgc tcgacgtagg acatcaggtg ggcgtgggcg
     4681 gcgcgcaccg cggggcgcgt gttgtacgcg cggatcccga gcacgaccgc ggtgtagcgc
     4741 gacaggtcgc cggtgcgcag cgcgtcgtcg tcgagctcgt cgacggtgag cccgacgtgc
     4801 gcgaggtcgg cggcgatcga gtcacccgag cccttgacgt aggcgatccg gccggcgggc
     4861 agcttgatcg acagcgggac cagcgccacc gtcgcgggcc gcagcacgac ctggaccggg
     4921 atgtgcgggt ggtcgatcac gtcctcgcgc agcgagtagg ccgcgtcgcc gatccggacc
     4981 accgggcgag cctcgccggc cgcggcgccg ggggccggcg tcacgtcgaa ctgcaccgtg
//
LOCUS       DASVOR010000482         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_442727,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000482 DASVOR010000000
VERSION     DASVOR010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442727"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1057)
                     /locus_tag="VF516_09600"
     CDS             complement(<1..1057)
                     /locus_tag="VF516_09600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8107970.1"
                     /translation="MVSRGGTMGTGAMRSQDGRASTDEVWSPEGGYLSVAKLRLEVID
                     GNDAGAARVFGRERIVIGSHDSADFVVRDSTVSRMHCELVPASGRVVVRDLCSKNGTA
                     IHGIFVREATLTGMAVLELGHTKIRISLGGEAAFLPLYDREQFGPLVGQCAAMRRAFA
                     LAATAAASDASVLIEGEAGTGKRTLAAAIHAQSARAGRPLAVLSCAGTPRELLETELF
                     GHAGDNRPGIFETVAGGSVVLDQVGDLPIELQPRLLAVLEHREVRRPGGEPIAVDARV
                     MATTGRNLAAGINGHRFRSDLHARLSAIQIALAPLRSRLDDLPLLVADLAPRLGLGAD
                     DRAHLTSQPLLAELGAHH"
BASE COUNT          192 a          558 c          564 g          206 t
ORIGIN      
        1 ggtgatgggc gccgagctcc gcgagcagcg gctgcgacgt caggtgggcg cggtcgtcgg
       61 cgccgagccc gagccggggc gcgaggtcgg cgaccagcag cggcaggtcg tcgagccggg
      121 agcgcagcgg cgccagcgcg atctggatcg ccgacagccg ggcgtggagg tccgagcgga
      181 agcgatggcc gttgatcccg gcggcgaggt tgcggccggt cgtcgccatc acccgcgcgt
      241 cgaccgcgat cggctcgccg ccgggccggc ggacctcgcg gtgctcgagg accgcgagca
      301 gccggggctg gagctcgatc ggcagatcgc cgacctggtc gagcaccacg ctgccgccgg
      361 ccacggtctc gaagatcccg ggccggttgt cgccggcgtg gccgaacagc tcggtctcga
      421 gcagctcgcg cggggtcccg gcgcacgaca gcacggccag cgggcggccg gcgcgcgcgc
      481 tctgcgcgtg gatcgcggcc gcgagcgtgc gcttgccggt cccggcctcg ccctcgatca
      541 gcacgctggc gtcggaggcg gccgcggtcg cggcgagcgc gaacgcccgg cgcatcgccg
      601 cgcactgtcc gaccagcgga ccgaactgct cgcggtcgta cagcggcagg aacgcggcct
      661 cgccgcccag gctgatccgg atcttggtgt ggccgagctc gagcaccgcc atgccggtga
      721 gcgtcgcctc gcgcacgaag atgccgtgga tcgccgtgcc gttcttgctg cacaggtcgc
      781 gcacgacgac gcggccgctc gccgggacca gctcgcaatg catgcgcgac accgtcgagt
      841 cgcgcaccac gaagtccgcc gagtcgtggc tgccgatcac gatccgctcg cggccgaaca
      901 cccgcgccgc gccggcgtcg ttgccgtcga tcacctcgag ccggagcttg gcgaccgaga
      961 gatagccgcc ctccgggctc cacacctcat cggtcgatgc gcggccgtcc tggctgcgca
     1021 tcgcccccgt ccccatcgtc ccgcctcgac tcaccatctt ccgtagtgta ctcgatgtgg
     1081 cgcgcggtgg ccgcgccggt ttcgcgcgcg aggtgtttcg cgtgtggaac accgtaccgg
     1141 tgcacggcgc acgcgcgcgg gcgcgcggcg tccggcgcgg cagcgcacgg cacccgagga
     1201 tgcactgtcc ctgcacggtc gcacgcgcag ctcacgttcg cggcggccgt gaccaggctt
     1261 tccggaagct gccaccggca gtagcccgtg ccgcgctccc ggccggaccg ctgccgcccc
     1321 ggccgccggc cggcgccgaa aacttggtgt cgatgtcagg tagtgtagga taggtctgcc
     1381 cggggagccg ctatgtctaa aaaccgaagg attcgtgaca cctccgtgag cccacgcgag
     1441 ggcggctcga ccggccgccg ccgcgaggtg ctcggcgtcg tgggcctggg ggcggcgatc
     1501 tttctcctga tcgcgctggt
//
LOCUS       DASVOR010000483         3469 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_209016,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000483 DASVOR010000000
VERSION     DASVOR010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3469
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209016"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..339)
                     /locus_tag="VF516_09605"
     CDS             complement(37..339)
                     /locus_tag="VF516_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX8107971.1"
                     /translation="MVLGKVIGTLVASRKEPTLEGLKLLVVRACDVDGSISAPGTGAV
                     VVAVDAVGAGIGEVVLYAAGSSARQTQVTQNRPVDATIMAIVDEIAVGGERRYVKD"
     gene            complement(348..638)
                     /locus_tag="VF516_09610"
     CDS             complement(348..638)
                     /locus_tag="VF516_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX8107972.1"
                     /translation="MIEVKGFVGMVEAADAMVKAAKVELVGYEKTGGGYVTAIVRGDV
                     AAVKAATEAGQRAAERVGEVVSVHVIPRPHANVDAVLPLGRASIQSGSDGKK"
     gene            complement(705..1343)
                     /locus_tag="VF516_09615"
     CDS             complement(705..1343)
                     /locus_tag="VF516_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107973.1"
                     /translation="MGTVLRTYCFLDALQPQQATFIGKTARGFLPVPFQASLWVEIAP
                     GIAINQVTDAALKATRVQPAVQVVERAYGLLEVHDHDKGEVLSAGATILRYLGVEEGQ
                     RLKPKVLTNQIIRGVEAYQTQIINRSSAGMMVLPGESLFILECEPAGYAVLAANEAEK
                     AAHVSLVNVTPYGAFGRLYMSGPEAEIDAAAAAATAALAGVTGKEPEKFVDK"
     gene            1463..3049
                     /locus_tag="VF516_09620"
     CDS             1463..3049
                     /locus_tag="VF516_09620"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8107974.1"
                     /translation="MRFSRLACLLFALIAGAPSGAHADVVGRALKAAEQQPGGGAAWQ
                     TPDLIEWAGEPRAITLPELLQITVRQAPNLAAAQIDIAVAEARIEQTWARNDWQVQAQ
                     LKGVRTGEVPQGTTTINNDTQIGGTFDLSRALPTGGTVDLHAQTGYDRLNYTVNLMVG
                     GQSFAQSGTSTTWTNDISGSITQPLLRKRGRRLFEANEIKATLARDVSVLARRLAAIQ
                     TVQTVISAYWDLVLAEQTVAITQGSLDLARERLRVTQIGADGGKVPRSEIPAVLQIIA
                     TRQEDVLSGELGVLNASIAMRRAAGMPIGPGELGLRVATDLDTRDQPINLGELVERAF
                     AASPELAQLAKQDASATIDVEVAEDGLLPQLDAALTLGPTGTGSSFSDSAKDLATFKS
                     IAITGTLTFTQSLGRHDALGRTREQRELKRKLTVNAADLRAQLAQTMSRSVAQLELAR
                     RRVTLSQRAIDLANENIKIETDRFNLGKSTNFDVLNRLEELRQAELRKAQALIDWRKA
                     EVVIQSLTGDLLPMYGISVD"
BASE COUNT          512 a         1316 c         1140 g          501 t
ORIGIN      
        1 atcttcttct cgtcgaggcc acccggaccc gccatcctag tccttcacgt agcggcgctc
       61 gccgccgacc gcgatctcgt cgacgatcgc catgatcgtc gcgtcgaccg ggcggttctg
      121 ggtcacctgg gtctggcgcg ccgacgagcc ggccgcgtac agcacgacct cgccgatccc
      181 ggcgccgacc gcgtcgaccg cgaccacgac ggcgcccgta ccgggggctg agatcgaccc
      241 gtcgacgtcg cacgcgcgga ccacgaggag cttcagccct tcgagcgtgg gctccttgcg
      301 gctcgcgacc agcgtgccga tgaccttgcc gaggaccacg gcgtctccta cttcttgccg
      361 tcgctgccgg actggatcga cgcccggccg agcggcagga cggcgtcgac gttggcgtgc
      421 ggccgcggga tcacgtggac cgacacgacc tcgccgacgc gctcggcggc gcgctggccg
      481 gcctcggtcg ccgccttgac cgcggcgacg tcgccgcgca cgatcgcggt cacgtagccg
      541 ccgccggtct tctcgtatcc gacgagctcg accttggcgg ccttgaccat cgcgtcggcg
      601 gcctcgacca tgccgacgaa acctttgacc tcgatcattc caagcgcgtc agccattcgt
      661 catctcctgc ttgtgctgct ttgctggtgc gttgccgctt ccggctactt gtcgacgaac
      721 ttctcgggct ccttgccggt gaccccggcg agcgccgcgg tcgccgccgc ggccgccgcg
      781 tcgatctccg cctccggccc cgacatgtag agccggccga acgcaccgta cggggtgacg
      841 ttgaccagcg agacgtgggc cgccttctcg gcctcgttgg ccgcgagcac cgcgtagccg
      901 gcgggctcgc actccaggat gaacagcgac tcgccgggca gcaccatcat gccggccgag
      961 ctgcggttga tgatctgggt ctggtaggcc tcgacgccgc ggatgatctg gttggtcagg
     1021 accttgggct tcaggcgctg gccctcttcg acgccgaggt atctgaggat cgtcgccccg
     1081 gccgacagca cctcgccctt gtcgtgatcg tggacctcga gcagcccgta cgcgcgctcg
     1141 acgacctgga ccgcgggctg gacccgcgtc gccttgagcg ccgcatcggt gacctggttg
     1201 atcgcgatgc cgggcgcgat ctcgacccac agcgaggcct ggaacgggac cgggagaaag
     1261 ccgcgcgcgg tcttgccgat gaacgtcgcc tgttgcggct gaagcgcgtc gagaaagcaa
     1321 tacgtacgca ggaccgtgcc catcgctgcc ggccacgcta acacacgcgg tttgcgcaaa
     1381 tccatgtcgc gtgcggcatt tgcccgtcgc acgtgtcgca cggtgcgcgc tgatccggtg
     1441 cgtgccatga tgccccgatt cgatgaggtt ttcacggctg gcttgcctgt tgtttgcctt
     1501 gatcgccggc gccccatcgg gcgcccacgc cgacgtcgtg ggtcgcgcgc tcaaggcggc
     1561 cgagcagcag ccgggcggcg gcgcggcatg gcagacgccc gatctgatcg agtgggccgg
     1621 cgagccccgt gcgatcaccc tgcccgagct gctccagatc accgtgcgcc aggcgccgaa
     1681 cctggccgcc gcccagatcg acatcgccgt cgccgaggcg cgcatcgagc agacctgggc
     1741 gcgcaacgac tggcaggtcc aggcccagct caagggcgtg cgcaccggtg aggtgcccca
     1801 gggcaccacc accatcaaca acgacaccca gatcggcgga acgttcgacc tgtcccgcgc
     1861 gctaccgacc ggcggcaccg tggatctgca cgcgcagacc ggctacgacc ggctcaacta
     1921 cacggtcaac ctcatggtcg gcggccagtc gttcgcgcag tccggcacca gcaccacatg
     1981 gaccaacgac atcagcggct cgatcacgca gccgctgctc cgcaagcgcg gccgccggct
     2041 gttcgaggcc aacgagatca aggcgacgct ggcccgcgac gtcagcgtgc tcgcccgccg
     2101 gctcgccgcg atccagaccg tgcagaccgt gatctcggcg tactgggacc tcgtgctcgc
     2161 cgagcagacc gtcgcgatca cccagggcag cctcgacctc gcgcgcgagc ggctgcgggt
     2221 cacccagatc ggcgccgacg gcggcaaggt cccgcgcagc gagatccccg cggtgctcca
     2281 gatcatcgcg acgcgccagg aggacgtgct gagcggcgag ctcggcgtgc tcaacgcgtc
     2341 gatcgcgatg cggcgcgccg ccggcatgcc gatcggcccc ggcgagctcg gcctgcgggt
     2401 cgccaccgat ctcgacaccc gggaccagcc gatcaacctc ggcgagctgg tcgagcgcgc
     2461 gttcgcagcg agccccgagc tcgcccagct cgccaagcag gacgccagcg cgacgatcga
     2521 cgtcgaggtc gccgaggacg gcttgctgcc ccagctcgat gccgcgctga cgctgggccc
     2581 gaccggcacc gggagctcgt tcagcgattc ggcgaaggac ctggccacgt tcaagtcgat
     2641 cgcgatcacc ggcacgctga ccttcaccca gtcgctcggc cggcacgacg cgctgggccg
     2701 cacccgcgag cagcgcgagc tcaagcgcaa gctcacggtc aacgccgccg acctccgcgc
     2761 ccagctcgcg cagaccatga gccgctcggt cgcccagctc gagctcgccc gccgccgcgt
     2821 cacgctgtcc cagcgcgcga tcgatctcgc caacgagaac atcaagatcg agaccgaccg
     2881 gttcaacctc ggcaagtcga ccaacttcga cgtgctgaac cggctcgagg agctgcgcca
     2941 ggccgagctg cgcaaggccc aggccctgat cgactggcgc aaggccgagg tcgtgatcca
     3001 gtcgctgacc ggcgacctgc tgccgatgta cggcatctcg gtcgactgat ccgctggtgg
     3061 ccggccccag gccccaggcc catgcgcgcg acctgcatcc cgcgggagcg cttttgccgt
     3121 ccgcgagcga cacggccggc tcggtgaacg gcgtgaatcc gcacgcttcg gcgtggcacg
     3181 ccagctgctc cagccgagcg ccatgcagcc catcgatccc ccggggggct ttgccccccg
     3241 ccccgccggc aaagccggca gggtccccac cccccatctc gcttcgctcg atcgtcagtc
     3301 tctcgcgtcc gtgaacggcg gcttcggcgc cctgctcggc gcactcctgc aagcggcccc
     3361 cggcatcctc cagggcgtca gcgggatcat cggcgccgcc aggagcggcg gaagctccgg
     3421 cggcagcccc gctgccggcc ccggcccgac gcaggcccag ccgcagcag
//
LOCUS       DASVOR010000484         4195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_267257,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000484 DASVOR010000000
VERSION     DASVOR010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4195
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267257"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1906)
                     /locus_tag="VF516_09625"
     CDS             complement(<1..1906)
                     /locus_tag="VF516_09625"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8107975.1"
                     /translation="MVGAGFAGLYMLHRLRQLGFSTRVLEAADDVGGTWYWNRYPGAR
                     CDVATTDYTYSFDPELEKAWTWSEKYATQPEILRYAQFVADRYDLRRDIEFGTRVAAA
                     RWDAASQRWHLHTASDQRIRCRHYVMATGCLSQPKMMDIPGAERFRGEVYFTSRWPHE
                     DVDFRGKRVAVIGTGSSGIQSIPLIARQAKQLTVFQRTPNFSIPAGNGPVRPERRAPL
                     EANREAYRTAAKWSRIGVPSTPTEVMAMYSPPEVHRQRFEAAWQSGELVELVNVFADQ
                     GVSAEANEILAEMVREKIRGLVNDPKTAELLCPKDHPIGTKRPCLDTDYYATFNQPHV
                     RLIDLRSHPIRTVTETGIALVDESLAFDAIVFATGFDAMTGAIVSVDIAGRDGVSLKQ
                     RWANGPLTYLGLMTVGFPNFYTITGPGSPSVLSNMMVSIEQHVDWIADCLVHLRAEGM
                     TTIEPTQTAEDGWMTHVNDCGQITLFSKADSWYVGANVPGKPRVLLPYAGGVDMYRKA
                     CDEVAARDYLGFARSGPAGTRCRDGVIRRVQPDVAMVLDLLAAAGLPPFESLPADAAR
                     GLLAQLSAARPTGPAVGEIRDGTLPGAAGPLAYRLYRPASPGPHPVVVYFHGGGWVLG
                     SHDSDDPLCRD"
     gene            complement(2065..2835)
                     /locus_tag="VF516_09630"
     CDS             complement(2065..2835)
                     /locus_tag="VF516_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8107976.1"
                     /translation="MTAINLPAVRAIYRFEMARTFRTLMQSIASPVLSTSLYFVVFGA
                     AIGSRMGQVGGVSYGAFIIPGLVMLSLLSESISNASFGIFLPKWSGTIYELLSAPVSF
                     VEVVLGYIGAAATKSVMLGALILITARLFVPYEIVHPVWAVGFLVLTAVTFSMFGFII
                     GLWADSFQKLQTIPLMVVTPLTFLGGAFYSISMLPPFWQRVTLFNPVVYLISGVRWAF
                     YGQSDVDAAISFVATLGFLAACLTFVWWVFRTGYKLKT"
     gene            complement(2832..3791)
                     /locus_tag="VF516_09635"
     CDS             complement(2832..3791)
                     /locus_tag="VF516_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8107977.1"
                     /translation="MQSSPQAHDAEAPPIVEIRGLSKTYASGFQALKRIDLTIRRGEI
                     FALLGPNGAGKTTLIHIVCGIVRPGDGSVLVGGCDIVREYRAARAKIGLVPQELSTDA
                     FETVWATVSFSRGLFGKRKNPAHIEKVLRALSLWDKRGSKIMTLSGGMKRRVLIAKAL
                     AHEPEILFLDEPTAGVDVELRHDMWQMVRELRQGGVTIILTTHYIEEAEQMADRIGVI
                     HQGELIVVEDKAALMRKLGKRQLVLHLQAALDRIPDALAGYPLELSADRETLTYTFDV
                     QREQTGIADLLRRLDELGIGFKDLRTSESSLEDIFVSLVRGRA"
     gene            complement(3884..>4195)
                     /locus_tag="VF516_09640"
     CDS             complement(3884..>4195)
                     /locus_tag="VF516_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107978.1"
                     /translation="WCESFGGAGGVQLITDPDRARYAAWGVGLSDRRHFAGPESLGAL
                     RRLLDEGIHNRWASGNRWQAAATFAVDPTGVLRWRHFPQHAGDLPPLADAIAALVGPP
                     S"
BASE COUNT          662 a         1411 c         1505 g          617 t
ORIGIN      
        1 ggtcgcggca cagcgggtca tcggaatcat gactgcccag cacccagccg ccgccgtgga
       61 agtagacgac gaccggatgc gggccggggc tcgcgggccg gtacagccgg taggcgagcg
      121 gacccgcggc gcccggaagc gtgccgtcgc ggatctcgcc caccgcgggg ccggtgggcc
      181 gtgcggccga tagctgggcc aggaggccgc gcgcagcgtc ggccggcagc gattcgaacg
      241 gaggcaggcc cgcggcggcc aggaggtcca gcaccatcgc cacgtcgggc tggacgcgcc
      301 ggatcacgcc atcgcggcac cgcgtgccgg ccggaccgct gcgcgcgaac cccaggtagt
      361 cgcgggcggc gacctcgtcg cacgccttgc ggtacatatc gacgccgccc gcatacggca
      421 ggagcacccg gggcttgccc ggcacgttgg cgcccacgta ccaggagtcg gccttcgaga
      481 acagcgtgat ctggccgcag tcgttgacgt gcgtcatcca gccgtcctcg gcggtctggg
      541 tcggctcgat cgtggtcatg ccctcggcgc gcaggtggac cagacagtct gcgatccagt
      601 cgacgtgctg ctcgatcgac accatcatgt tcgacagcac cgaggggctg cccgggccgg
      661 tgatggtgta gaagttggga aacccgaccg tcatcaggcc caggtaggtg agcggaccat
      721 tcgcccatcg ctgcttgagc gagaccccgt cgcgccccgc gatgtccacc gacacgatgg
      781 cgccggtcat cgcgtcgaag ccggtcgcga acacgatcgc gtcgaacgcc aggctctcgt
      841 cgaccagcgc gatgccggtc tcggtgacgg tccggatcgg atggctgcgc aggtcgatca
      901 gccgcacgtg cggctggttg aacgtcgcgt agtagtcggt gtccaggcac gggcgcttgg
      961 tcccgatcgg atggtccttg gggcacagca gctcggcggt cttggggtcg ttgaccaggc
     1021 cgcggatctt ctcgcgcacc atctcggcca ggatctcgtt ggcctcggcc gagacgccct
     1081 ggtccgcgaa cacgttgacc agctcgacga gctctccgga ctgccacgcc gcctcgaagc
     1141 gctggcggtg gacctcgggg ggcgagtaca tcgccatcac ctcggtcggg gtgctcggca
     1201 cgccgatccg cgaccacttc gccgccgtgc ggtaggcctc gcgattcgct tccagcgggg
     1261 cgcgccgctc gggccgcacc ggaccattgc cggccgggat ggagaagttg ggcgtgcgct
     1321 ggaacacggt gagctgcttg gcctggcgcg cgatcagcgg aatcgactgg atgcccgacg
     1381 agccggtgcc gatcacggcc acgcgcttgc cgcggaagtc gacgtcctcg tggggccagc
     1441 ggctggtgaa gtagacctcg ccgcggaacc gctcggcacc ggggatgtcc atcatcttgg
     1501 gctgcgacag gcagccggtg gccatcacgt agtggcggca gcgaatgcgc tggtcgctcg
     1561 cggtgtgcag gtgccagcgc tggctggcgg cgtcccagcg agcggccgcc acgcgggtgc
     1621 caaactcgat gtcgcgccgc agatcgtagc gatcggcgac gaactgggcg tagcgcagga
     1681 tctcgggctg ggtcgcgtac ttctccgacc aggtccacgc cttctcgagc tcggggtcga
     1741 agctgtaggt gtagtcggtg gtggcaacgt cgcagcgcgc ccccggatag cggttccagt
     1801 accaggtgcc gccgacgtcg tccgccgcct cgagcacgcg ggtcgagaac cccagctgcc
     1861 gcagccggtg cagcatgtag agtcccgcga agccagcgcc caccacgacc acatcgagtt
     1921 catcgggccg agatgtatca ggattcgtca tgcatctccc gcttgtgcct ggcgacagcg
     1981 ccgcgctgtc gccagcagca ctgttgcacg actcccggcc cggccgtcca ggattgttcg
     2041 aatcgctctg cttccggcgc tcgctcaggt cttgagcttg tagccggtcc ggaacaccca
     2101 ccacacgaac gtcaggcagg cggcgaggaa gccgagcgtg gcgacgaagc tgatcgcggc
     2161 gtcgacgtcg gactggccgt agaacgccca gcggacgccg gagatcaggt agacgaccgg
     2221 gttgaacagc gtgaccctct gccagaacgg cggcagcatg ctgatcgagt agaacgcacc
     2281 gccgaggaag gtcagcgggg tcaccaccat cagcgggatg gtctgcagct tctggaagct
     2341 gtcggcccac agcccgatga tgaagccgaa catcgagaac gtcaccgcgg tcagcacgag
     2401 gaagccgacc gcccacaccg ggtggacgat ctcgtagggc acgaacagcc gcgcggtgat
     2461 caggatcagc gcgccgagca tgaccgactt ggtcgccgcc gcgccgatgt agccgagcac
     2521 gacctcgacg aacgacaccg gcgcggacag cagctcgtag atcgtaccgg accacttggg
     2581 caagaagatg ccgaacgacg cgttggagat gctctcgctg agcagcgaca gcatgaccag
     2641 gccgggtatg atgaaggcgc cgtaggacac gccgccgacc tggcccatcc gcgagccgat
     2701 cgccgcgccg aacaccacga agtacagcga ggtcgacagc accggcgacg cgatgctctg
     2761 catcagggtg cggaacgtgc gcgccatctc gaaccggtag atcgcgcgga ccgcggggag
     2821 gttgatcgcg gtcatgcgcg cccccggacc aggctgacga agatgtcctc gagcgagctc
     2881 tcgctcgtcc gcaggtcctt gaagccgatg ccgagctcgt cgagccggcg cagcaggtcg
     2941 gcgatcccgg tctgctcgcg ctggacgtcg aacgtgtagg tcagggtctc gcggtccgcc
     3001 gacagctcga gcgggtagcc ggcgagcgcg tcggggatcc ggtcgagcgc ggcctggagg
     3061 tggagcacga gctggcgctt gccgagcttg cgcatcagcg cggccttgtc ctcgacgacg
     3121 atcagctcgc cctggtggat cacgccgatc cggtcggcca tctgctcggc ctcctcgatg
     3181 taatgcgtgg tgaggatgat cgtcacgccg ccttggcgca gctcgcgcac catctgccac
     3241 atgtcgtggc ggagctcgac gtcgacgccg gcggtcggct cgtcgaggaa caggatctcc
     3301 ggctcgtgcg ccagcgcctt ggcgatcagc acgcggcgct tcatgccgcc cgacagcgtc
     3361 atgatcttgc tcccgcgctt gtcccacagc gacagcgcgc gcagcacctt ctcgatgtgc
     3421 gccgggttct tgcgcttgcc gaacaggccg cggctgaagc tgacggtggc ccacacggtc
     3481 tcgaacgcgt cggtcgacag ctcctgcggc accaggccga tcttggcgcg cgcggcgcga
     3541 tactcgcgga cgatgtcgca cccgccgacg agcacgctgc cgtcgccggg gcgcacgatg
     3601 ccgcagacga tgtggatcag cgtggtcttg ccggcgccgt tggggccgag cagcgcgaag
     3661 atctcgccgc ggcggatcgt caggtcgatg cgcttcagcg cctggaagcc gctggcgtag
     3721 gtcttgctga gtccgcggat ctcgacgatc ggaggggcct cggcatcgtg ggcctggggg
     3781 gaggactgca tgggcccgaa aaccttgcgc ccgccgcccc gcatcgcaac cggtgcgcgg
     3841 cggtaaaagt tgccgggggg ctgtcagctt tcatgcggga tcgtcaggac ggcgggccga
     3901 ccagcgcggc gatggcgtcg gcgagcggcg gcagatcgcc ggcgtgctgc gggaagtggc
     3961 gccagcgcag cacgccggtg gggtcgacgg cgaacgtcgc ggcggcctgc cagcggttgc
     4021 cgctcgccca gcggttgtgg atgccctcgt cgagcaggcg gcgcagcgcg ccgagcgact
     4081 cggggccggc gaagtggcgg cgatcgctga ggccgacgcc ccacgcggcg tagcgcgcgc
     4141 gatcgggatc ggtgatcagc tggacgccgc ccgccccgcc gaacgactcg cacca
//
LOCUS       DASVOR010000485         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238081,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000485 DASVOR010000000
VERSION     DASVOR010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238081"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VF516_09645"
     CDS             <1..573
                     /locus_tag="VF516_09645"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8107979.1"
                     /translation="RQILSAAKAVFAEAGYHGASIHAIIERAQIARGTFYLYFESKAA
                     VFDSILDQAMADLRARLHRIEVDAPGAPPPQVQLRDQVVATLEYIMNDRPLATLLLSA
                     GHTPDVDASERLEMFFTEVRDLLRRALETGMDLGLVRRCPSELVAAALLGMIRGVIEL
                     MVRQTDAARPMRVDEVVTEMLQVALRGVLA"
     gene            752..>1774
                     /locus_tag="VF516_09650"
     CDS             752..>1774
                     /locus_tag="VF516_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX8107980.1"
                     /translation="MSTPDQFVIGIDVGSTTVKMTVVDPGTKEILWSKYQRHETRQPE
                     MTRDMLREIHDAFPAVTKDRIRVFITGSGGGPIASQIGAKFVQEVNAVTMAVEKLHPD
                     VGSVIELGGQDAKIIMFKQNNETGDKTAQTSMNDKCASGTGATIDKCVLKVGMPREEV
                     PRLKFDPTKLHHVAAKCGVFAETDIVNLVKSGIPRNEIMNSLADAIVSQNLAVLTRGN
                     TLKAKVLLLGGPNTYLPFLQECWRLRIPEAWAERGYEYDKTRPIEELIFVPKNSDLYA
                     AYGAVLFGLYEPESVGRYRGLEPLEEFITHGRKSKLGESAGPGLVETPEQRDAFADKY
                     RTPDYAD"
BASE COUNT          314 a          615 c          581 g          264 t
ORIGIN      
        1 cgccagatcc tgtcggcggc caaggcggtg ttcgccgagg ccggctacca cggcgcgtcg
       61 atccacgcga tcatcgagcg cgcccagatc gcgcgcggca cgttctacct gtacttcgag
      121 agcaaggccg cggtgttcga ctcgatcctc gaccaggcga tggccgacct gcgcgcccgg
      181 ctgcaccgga tcgaggtcga cgcgcccggc gccccgccgc cccaggtcca gctccgcgac
      241 caggtcgtgg cgacgctcga gtacatcatg aacgaccggc cgctcgcgac cctgctgctg
      301 tcggcgggcc acaccccgga cgtcgacgcg agcgagcggc tcgagatgtt cttcaccgag
      361 gtccgcgatc tcctgcgccg cgcgctcgag accggcatgg acctcggcct ggtccggcgc
      421 tgcccgtccg agctggtcgc cgccgcgctg ctcgggatga tccgcggcgt gatcgagctg
      481 atggtgcgcc agaccgacgc cgcgcggccg atgcgcgtgg acgaggtcgt gacggagatg
      541 ctgcaggtcg cgttgcgcgg cgtactcgcg tagcgcgggc tgtggcggct ttgccctgtg
      601 cgcccatgtc attggcccga ccattcggcc aattcgagaa aagatctgga tctgtccgca
      661 cttgctacta gactgacacg tcagtcatct ctagactgac gcgtcagtct agtcctgcgt
      721 ccgacgcaac ctctgcgtca gcgagaagct catgagcaca cccgaccagt tcgtgatcgg
      781 catcgacgtc ggctccacga ccgtgaagat gacggtcgtc gatccaggca ccaaggagat
      841 cctgtggtcg aagtaccagc gccacgagac ccggcagccc gagatgacgc gcgacatgct
      901 gcgcgagatc catgacgcgt tcccggcggt gaccaaggat cggatccggg tgttcatcac
      961 cggctccggc ggcggaccga tcgcgtcgca gatcggcgcg aagttcgtcc aggaggtcaa
     1021 cgcggtgacc atggcggtgg agaagctcca ccccgacgtc ggcagcgtga tcgagctcgg
     1081 cggccaggac gccaagatca tcatgttcaa gcagaacaac gagaccggcg acaagaccgc
     1141 gcagacgtcg atgaacgaca agtgcgcgtc gggcaccggc gcgacgatcg acaagtgcgt
     1201 gctcaaggtc ggcatgccgc gcgaggaggt cccccggctc aagttcgatc cgaccaagct
     1261 gcaccacgtc gccgccaagt gcggcgtgtt cgccgagacc gacatcgtca acctggtgaa
     1321 gtcgggcatc ccgcgcaacg agatcatgaa ctcactggcc gacgcgatcg tcagccagaa
     1381 cctcgcggtc ctgacccgcg gcaacacgct caaggccaag gtgctcttgc tcggcgggcc
     1441 caacacctac ctgccgttcc tccaggagtg ctggcgcctc aggatccccg aggcgtgggc
     1501 cgagcgcggc tacgagtacg acaagaccag gccgatcgag gagctgatct tcgtccccaa
     1561 gaactccgac ctgtacgccg cgtacggcgc ggtgctgttc gggctgtacg agcccgagtc
     1621 ggtcggccgc taccgcgggc tcgagccgct cgaggagttc atcacccacg gccgcaagag
     1681 caagctcggc gagtcggccg gccccggcct ggtcgagacc cccgagcagc gcgacgcgtt
     1741 cgccgacaag taccggacgc cggactacgc cgac
//
LOCUS       DASVOR010000486         2940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_62912,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000486 DASVOR010000000
VERSION     DASVOR010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62912"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF516_09655"
     CDS             <1..327
                     /locus_tag="VF516_09655"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8107981.1"
                     /translation="GRRERRLTHDAARALFAHPWPFDIRELEQTLRGAVAVADGRAID
                     VDDLRLTAPPAEPRPVGGRQALASALQRHAGNLSAVARELATSRSQVQRLLDRYELSA
                     DEFKRG"
     gene            628..1206
                     /locus_tag="VF516_09660"
     CDS             628..1206
                     /locus_tag="VF516_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEX8107982.1"
                     /translation="MNIKLQHMITVGVTLGMAGCLDQGPVADADATAELAEPPAAITQ
                     QILAGAAASTFHQIKNRLYVQCMDAPGGAFNVILKLANCNFLSEQHWAFVAAPAPNTF
                     FLVNQLTGLCAEVNNGTAIPGERVDEFTCNGSTAEQWVSNLFNVGGTVYQQFQHAGTN
                     QCLDTVGAQGSQLMQFNCDLTGQNQAQQWLVQ"
     gene            1302..1436
                     /locus_tag="VF516_09665"
     CDS             1302..1436
                     /locus_tag="VF516_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107983.1"
                     /translation="MTRICNSVEQPRRRKLMTIEEGRTLIERFRHGMGGYTCLERGRM
                     "
     gene            complement(1468..>2940)
                     /locus_tag="VF516_09670"
     CDS             complement(1468..>2940)
                     /locus_tag="VF516_09670"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEX8107984.1"
                     /translation="PDPGAVHRFLRWLIDARSEAWRAEALAAPDVLMITQPPHRPAEP
                     RLIVASNRLPASSNGRQREVGGLVSALLPVLTETDGIWLGWSGAEREPGLRLRTESAD
                     AITRAQFDYPPTWRQRFYAGFCNQSLWPLLHGFRSRARFVDDEWQCYIEANRAYADKV
                     LEVGGAGATVWIQDFHLMLVGRELRRAGHHGRIGFYLHVPFPSLDEFETMPWASEVMS
                     ALLDFDLIGLQSLRWRDNFIATARALLGSEAEARAHDRGRVIGVGIDPERFAEAAAQT
                     PDNGELGTFDPMLRGRKLILGVDRLDYSKGIPERLEAFARLLERFPEWRGKVSFVQVS
                     VPTRSEVPEYAELRARVELLVGRINGAFGEADWTPVSYLYRSYDQATLARLYRLAAVA
                     LVTPLRDGMNLVAKEYVAAQDPADPGVLVLSRFCGAAEQMALALLTNPYHVDGVAADL
                     DTALRMSLDERITRHAALRASVWQDTASAWAKTFLAALAD"
BASE COUNT          454 a         1020 c         1074 g          392 t
ORIGIN      
        1 ggccggcggg agcgccggct gacccacgac gcggcgcgtg cgctgttcgc gcacccgtgg
       61 ccgttcgaca tccgcgagct cgaacagacc ctgcgcggcg cggtcgcggt cgccgacgga
      121 cgggcgatcg acgtcgacga cctccggttg acggcgccgc cggcggagcc caggcctgtc
      181 ggcggacgac aggccctggc ctcggcgcta cagcgtcatg caggcaacct cagtgcggtg
      241 gctcgcgagc tggcgacgtc gcggagccag gtccagcgac tgctcgatcg ctacgagctg
      301 agcgcggacg agttcaagcg cggctgaacc gccagggcac ggcggtgcac tgcgaaccag
      361 caggggtccc tgcttctcac tcaaggacgc cgtcgatccc ataaagaact cgctcgagct
      421 gagcatggcg gccgctccgg tacaccggcg gtgcaccgcc ggcctcgccg cgcaagccgg
      481 gaggcgagcg cgctcgcggg gttggcgcgc ggggggcgcc ggcccggaag ttgctgttgg
      541 ccggcctgca caccgcgtcg atcctttcgt cgatcctcgg cggggcggat cgcgcggcga
      601 gatctatcac gaagcgagag gtacgatatg aacatcaagc tccagcacat gatcaccgtc
      661 ggcgtcacgc tcggcatggc cggctgcctc gaccagggcc cggtcgcgga cgcggacgcc
      721 accgccgaac tcgccgagcc gcccgccgcg atcacccagc agatcctcgc cggcgccgcc
      781 gccagcacct ttcaccagat caagaaccgc ctgtacgtcc agtgcatgga cgcgcccggt
      841 ggcgcgttca acgtgatcct caagctcgcc aactgcaact tcctgagcga gcagcactgg
      901 gcgttcgtgg ccgcgccggc gccgaacacg ttcttcctgg tcaaccagct caccgggctg
      961 tgcgccgagg tcaacaacgg caccgcgatc ccgggcgagc gcgtcgacga gttcacgtgc
     1021 aacggctcga ccgcggagca atgggtgtcc aacctgttca acgtcggcgg caccgtctac
     1081 cagcagttcc agcacgccgg caccaaccag tgcctggaca cggtcggcgc gcagggtagc
     1141 cagctgatgc agttcaactg cgacctgacc ggccagaatc aggcgcagca gtggctcgtg
     1201 cagtgatcaa ttgtcgatcg cgatccagcc gtgcccttgc ggcgtagatg cgtgaagcag
     1261 cggtcagggc ttgatacgct cgacgggcct ggtccagggc catgacccgg atctgcaaca
     1321 gcgtcgagca gccgcggcgc aggaagctga tgacgatcga ggagggccgc acgctgatcg
     1381 agcggttccg ccacgggatg ggcggttaca cctgcctcga gcgcggccgg atgtgatcgc
     1441 gccgctgcgg caggtagcgt cgccgcgtca gtcggcgagc gcggcgagga aggtcttggc
     1501 ccacgcgctc gcggtgtcct gccagaccga ggcgcgcagc gcggcgtggc gggtgatccg
     1561 ctcgtcgagc gacatccgca gcgcggtgtc gaggtcggcg gcgacgccgt cgacgtggta
     1621 cgggttggtg agcagcgcga gcgccatctg ctcggcggcg ccgcagaacc gcgacagcac
     1681 gagcacgccg gggtcggccg gatcctgggc cgcgacgtac tccttggcga ccaggttcat
     1741 gccgtcgcgc agcggggtga ccagcgcgac cgcggcgagc cggtacagcc gggccagggt
     1801 cgcctggtcg tacgagcggt acaggtagct gaccggggtc cagtcggcct cgccgaacgc
     1861 gccgttgatc cggccgacca gcagctcgac ccgggcgcgc agctcggcgt actcggggac
     1921 ctccgagcgg gtcggcaccg agacctggac gaacgacacc ttgccgcgcc actcggggaa
     1981 ccgctcgagc aggcgggcga acgcctcgag ccgctcgggg atgcccttgg agtagtcgag
     2041 ccggtcgacg ccgaggatca gcttgcggcc gcgcagcatc ggatcgaatg tgccgagctc
     2101 gccgttgtcg ggggtctggg ccgcggcctc ggcgaatcgc tcgggatcga tgccgacgcc
     2161 gatcacccgg ccgcggtcgt gcgcgcgggc ctcggcctcg ctgccgagca gggcgcgcgc
     2221 ggtggcgatg aagttgtcgc gccagcgcag cgactgcagg ccgatcaggt cgaagtcgag
     2281 cagcgccgac atgacctcgc tcgcccacgg catggtctcg aactcgtcga gcgacgggaa
     2341 cgggacgtgg agatagaacc cgatccggcc gtggtgtccg gcgcgccgca gctcgcggcc
     2401 gaccagcatg aggtggaagt cctggatcca gaccgtcgcg ccggcgccgc cgacctcgag
     2461 caccttgtcg gcgtaggcgc ggttggcctc gatgtagcac tgccactcgt catcgacgaa
     2521 ccgcgcccgg ctgcggaagc cgtggagcag cggccacagg ctctggttgc agaacccggc
     2581 atagaaccgc tggcgccagg tcggcgggta gtcgaactgc gcgcgggtga tcgcgtcggc
     2641 ggactcggtg cgcagccgca acccgggctc gcgctcggcg ccgctccagc ccagccagat
     2701 cccgtcggtc tcggtcagca ccggcaacag cgccgagacc aggccgccga cctcgcgctg
     2761 gcggccgttc gagctcgcgg gcagccggtt cgacgcgacg atcaggcgcg gctcggccgg
     2821 gcgatgcggc ggctgggtga tcatcaggac gtcgggcgcg gcgagcgcct cggcgcgcca
     2881 ggcctcgctg cgcgcgtcga tcagccagcg caggaagcgg tgcacggcgc cggggtcggg
//
LOCUS       DASVOR010000487         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501337,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000487 DASVOR010000000
VERSION     DASVOR010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501337"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(361..1494)
                     /locus_tag="VF516_09675"
     CDS             complement(361..1494)
                     /locus_tag="VF516_09675"
                     /inference="COORDINATES: protein motif:HMM:NF020932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EfeM/EfeO family lipoprotein"
                     /protein_id="HEX8107985.1"
                     /translation="MRRSPATASGRSTARTVLGHDLLPVACVALAGLTGCLAKTDADF
                     RAEVTASMHTAMTQNLADMVQAARELQAAAPIRGWNVVTDAAAVNKMRDAWRRVRVAW
                     EQLEGAVTPMFPDLDASMDSRYEDFLLAPGAQGDLYLFDAHGVIGMHAIERILFAPIT
                     RSEVVAFESTLPGYKQSAYPATDNEAVAFKTQLVQRLIDDATQLQSDWKPEDLNIAAA
                     YQGLVSLMAEQRDKVNLAATGAEESRYSNITLADLRNNLAGTHTAYDLFREWIYSKSS
                     AVSSDDKIQARFSTLKDVYSTTTTTSGDALPEVPAGWDPANPSPDALKTPYGTLWREV
                     HASVDPNSEGSVVFEMKKIGELLGFTGAAGIADPPMLIGQRLR"
     gene            1627..2031
                     /locus_tag="VF516_09680"
     CDS             1627..2031
                     /locus_tag="VF516_09680"
                     /inference="COORDINATES: protein motif:HMM:NF042704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-phosphate processing receiver
                     domain-containing protein"
                     /protein_id="HEX8107986.1"
                     /translation="MNYVLFLDDDRWRHGRFQARVEALGYARRHRLLYVFTAADAIDA
                     LRAHEGEIATAFLDHDLCEDDHWVKPGAPSKAPTGMVVVDHIVTMRQPPPAITVHSFN
                     HHAAVAMCARLSALRGVSVHYIPFDVLLGQLA"
     gene            complement(2019..>2364)
                     /locus_tag="VF516_09685"
     CDS             complement(2019..>2364)
                     /locus_tag="VF516_09685"
                     /inference="COORDINATES: protein motif:HMM:NF023128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8107987.1"
                     /translation="FVLGGAGYAPLVLASSADAPYLAIAGIALWSITRSATESIAKAL
                     IAAIVPRGQRGRAYGLYYLVWGIAWWAGSLVLGALYDRGHMAAAAVAVVALAAGAAVV
                     ALSARARSSQAS"
BASE COUNT          348 a          849 c          823 g          344 t
ORIGIN      
        1 ggtcagcgcg tccgcgcgcc agtctccgtc cggatggccg gcgaccgcga cctcgagcac
       61 gacgctgcgc cgggccgcgg cgcgcgcggt cgccggccag agcacggcga ggcccccggc
      121 gagcaccgcc caggcgatcc cggcgagcac agagctgcga cacaaccgat ccacggctgg
      181 ccaagcccta tcacgcccct cccacagtca cgggcgccgc cccgcggcgc agggcgcgca
      241 gaccgcggcg atcgctgccc aaatcgggga ggggcggagc cccttgggcc aaagcgggga
      301 ggggaccccg cgggcttgcc cgtggggagg ggcggagccc ctcgggcccc tcgggatcgt
      361 tcagcggagg cgctgcccga tcagcatcgg cgggtcggcg atgccggcgg ccccggtgaa
      421 tccgagcagc tcgccgatct tcttcatctc gaacacgacc gagccctcgc tgttcggatc
      481 gaccgaggca tggacctccc gccacagcgt gccgtacggt gtcttgagcg catcggggga
      541 cgggttcgcc ggatcccagc cggccggcac ctcgggcagc gcgtcgccgc tggtcgtcgt
      601 cgtcgtcgag tacacgtcct tgagcgtcga gaaccgggcc tggatcttgt catccgagct
      661 caccgccgac gacttcgagt agatccattc gcggaacagg tcgtaggcgg tgtgggtccc
      721 ggcgaggttg ttgcgcagat cggccagcgt gatgttcgag taccgcgatt cctcggcgcc
      781 ggtcgcggcg aggttgacct tgtcccgctg ctccgccatc agcgagacca ggccctggta
      841 cgcggcggcg atgttgaggt cctccggctt ccagtccgac tggagctggg ttgcgtcgtc
      901 gatcaggcgc tgcacgagct gggtcttgaa cgcgaccgcc tcgttgtcgg tcgcggggta
      961 cgcagactgc ttgtagccgg gcagcgtgct ctcgaacgcg acgacctcgg agcgcgtgat
     1021 cggggcgaac aggatgcgct cgatcgcgtg catcccgatc acgccatggg cgtcgaacag
     1081 gtacagatcg ccctgggcgc cgggcgccag gaggaagtcc tcgtagcgcg agtccatcga
     1141 ggcgtcgagg tcggggaaca tcggggtgac cgcgccctcg agctgctccc acgcgacgcg
     1201 cacgcgcctc caggcgtcgc gcatcttgtt gaccgccgcg gcgtcggtca cgacgttcca
     1261 cccgcggatc ggagccgcgg cctggagctc gcgcgcggcc tgcaccatgt ccgccaggtt
     1321 ctgggtcatc gcggtgtgca tcgacgccgt gacctcggcc cggaagtcgg catcggtctt
     1381 ggccaggcac ccggtcagcc ctgccagcgc aacgcacgcg accggcaaca ggtcgtgccc
     1441 gaggacggtc cgcgcagtgc tccgaccgct ggccgtcgct ggggaacgtc gcacgctgtt
     1501 cacgttccca ttgaagtcca atcctccgcc accggtcaag agggacccgg tgtagcggtc
     1561 tgctgcatgc cacctgccat ggaccagctt cacaggacga atgctccaag gggggtatcc
     1621 tcgtacatga attacgtcct gttcctcgat gacgaccgct ggcgtcatgg gcgcttccag
     1681 gcgcgggtcg aggcacttgg ctacgcccgc cgccatcgcc tgctctacgt cttcaccgcc
     1741 gctgacgcga tcgatgcgct gcgggctcac gagggtgaga tcgccacagc gttcctcgat
     1801 cacgacctat gtgaggacga ccactgggtg aagcccggcg cgcccagcaa ggccccgacg
     1861 gggatggtcg tggtcgacca catcgtgacg atgcgccagc cgccgccggc gatcaccgtg
     1921 cacagcttca accaccacgc ggccgtcgcg atgtgcgccc ggctgtcggc gctgcgcggc
     1981 gtgtcggtcc actacatccc gttcgacgtg ctgctcggtc agctcgcctg actggatcgg
     2041 gcgcgggccg acagcgcgac gaccgcggca ccggcggcca gggcgaccac cgccaccgcc
     2101 gcggcggcca tgtgcccccg gtcgtagagc gcgcccagca cgaggctccc ggcccaccac
     2161 gcgatgcccc acaccaggta gtacaggcca taggcgcggc cgcgctggcc gcggggcacg
     2221 atcgcggcga tcagcgcctt ggcgatcgac tcggtggcgg accgcgtgat cgaccacagc
     2281 gcgatcccgg cgatcgccag gtacggcgcg tcggcgctcg acgccagcac gagcggcgcg
     2341 tagccggccc cgccgagcac gaac
//
LOCUS       DASVOR010000488         2830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_33525,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000488 DASVOR010000000
VERSION     DASVOR010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33525"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VF516_09690"
     CDS             <1..864
                     /locus_tag="VF516_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107988.1"
                     /translation="RGRRARPRRRVPTAKPLRPIGAAGLLWRFMNYSTPYTVFGGLCE
                     AIPGVLLLWRRTAVIGAVMVIAVMTNVVMLNLSYDVPVKLYSMQLLVMAALIALPDAR
                     RLLAAALGRATAEVPARVRMSPRRERVRQVAKAIFVLGLAVSLYPTLGGRNPPSDVFG
                     NWVVDMLTADGVEHPPLATDPVRWQTVAFSNGRMAIWRVSGDRDPQLTASRGTYPYQL
                     DAASHTIRVTIDPEKKLEETWQYARPAPDHLVIDGVHLGASLHVALHAAPEPLLTTRG
                     FHWINEAPFSR"
     gene            complement(907..1440)
                     /gene="def"
                     /locus_tag="VF516_09695"
     CDS             complement(907..1440)
                     /gene="def"
                     /locus_tag="VF516_09695"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX8107989.1"
                     /translation="MAVRPVVIWPDERLRQPSVAVTAIDASVRQLYQDLVDSMYAENG
                     LGIAALQLGDPRRMFIVEPKLAGRPETDPPLAFINPEVIETSEEQQDSEEGCLSFPEV
                     YIKVKRPMRCKVRAMGLDGAMFEIAGEALFARCMLHENDHLTGKLLVDFVGPLKRQMI
                     KKKLQKQKAAEADEAEA"
     gene            1509..2123
                     /gene="ribA"
                     /locus_tag="VF516_09700"
     CDS             1509..2123
                     /gene="ribA"
                     /locus_tag="VF516_09700"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEX8107990.1"
                     /translation="MVLRYSEARLPTPHGEFRVVVYRTGQGAGPGATAVGTVTEEHVA
                     MVLGDIGGADDILTRVHSSCFTGEVLGSLRCDCRAQLDAALARIGREGRGVVVYLVQE
                     GRGIGLGNKVRAYQLQDDGADTVDANLQLGFDADLRSYDLAARILADLGVASVRLMTN
                     NPQKISGLERAGVVVAAHEPHWVAATEHSAGYLAAKKTKLGHLV"
     gene            2143..>2830
                     /locus_tag="VF516_09705"
     CDS             2143..>2830
                     /locus_tag="VF516_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEX8107991.1"
                     /translation="MITEAAVKQALTQVIDPALGHDIVGYRMVRSVEVQGDDAMIRID
                     VPTHAYPLARRRELVVQIEQAVKALGAKRVTVIPEVVTAFLPAPSDKAMLKGPKNVIA
                     VAAGKGGVGKSTVAVNLALALARHGAKVGLLDADVFGPSIPTMLGAPEVPASATKDSR
                     ITPAVHHGLRGISVGVFVDKGETVVWRGPMVHRLLQQFLGDVDWGELDYLVCDLPPGT
                     GDVQLSLSQLI"
BASE COUNT          415 a          983 c         1004 g          428 t
ORIGIN      
        1 cggggacgcc gtgcgcggcc acggcgccgg gtgcccacgg cgaaaccgct gcgaccgatc
       61 ggggccgccg gcctgctctg gaggttcatg aattactcga cgccgtacac ggtgttcggc
      121 gggctgtgcg aggcgatccc cggggtcctg ctgctgtggc ggcggaccgc ggtgatcggt
      181 gcggtgatgg tgatcgcggt gatgaccaac gtcgtgatgc tcaacctcag ctacgacgtg
      241 ccggtcaagc tgtactcgat gcagctcctg gtcatggccg cgctgatcgc gctgcccgac
      301 gcacgccggc tgctcgcggc ggcgctcggc cgcgcgaccg ccgaggtccc ggcccgcgtg
      361 cggatgtcgc cgcggcggga gcgcgtgcgc caggtcgcca aggcgatctt cgtcctcggg
      421 ctggcggtga gcctgtaccc gacgctgggc ggccgcaacc cgccctcgga cgtgttcggc
      481 aactgggtgg tcgacatgct caccgccgac ggggtcgagc atccgccgct ggccacggat
      541 ccggtgcgct ggcagacggt ggcgttcagc aacggtcgca tggcgatctg gcgcgtgtcc
      601 ggggaccgcg atccgcagct caccgccagc cgcggcacct atccctacca gctcgatgcg
      661 gcgagccaca cgatcagggt gacgatcgac cccgagaaga agctcgagga gacctggcag
      721 tatgcccgcc cggcgcccga ccacctggtg atcgacggcg tgcacctcgg cgcgtcgctg
      781 cacgtcgcgc tgcacgccgc gcccgagccg ctgctcacga cccgggggtt ccactggatc
      841 aacgaggcgc cgttcagccg gtgagctggc cggtgacccg gcccctgacc cggtggtgac
      901 ccggggctac gcctcggcct cgtcggcctc ggccgccttc tgcttctgca gcttcttctt
      961 gatcatctgg cgcttgagcg ggccgacgaa gtcgaccagc agcttgccgg tcaggtgatc
     1021 gttctcgtgc agcatgcagc gcgcgaacag cgcctcgccc gcgatctcga acatcgcgcc
     1081 gtcgaggccc atcgcgcgga ccttgcagcg catcgggcgc ttgaccttga tgtagacctc
     1141 ggggaacgac aggcagcctt cctcgctgtc ctgctgctcc tcggaggtct cgatcacctc
     1201 ggggttgatg aacgcgagcg gcggatcggt ctcgggccgg ccggcgagct tgggctcgac
     1261 gatgaacatc cggcgcgggt cgccgagctg gagcgcggcg atgcccaggc cgttctcggc
     1321 gtacatcgaa tcgaccaggt cctggtacag ctgtcggacc gacgcgtcga tggcggtgac
     1381 cgccaccgac ggttgccgca accgctcgtc gggccagatc accacggggc gtaccgccat
     1441 gggtccacca taggtgccgc cggcgagatt gacaagcccc gccgcgagcg ccatatacgc
     1501 ctaatcacat ggttctgcgg tactcggaag cgcgcttacc caccccgcat ggcgaattcc
     1561 gggtcgtggt ctaccggacc gggcaggggg ccggccccgg ggcgaccgcc gtcggcaccg
     1621 tcaccgagga gcacgtggcg atggtgctcg gcgacatcgg cggggccgat gacatcctga
     1681 cccgggtcca cagctcgtgt ttcaccggcg aggtgctcgg cagcctgcgc tgcgactgcc
     1741 gggcgcagct ggatgcggcg ctggcgcgga tcggccgcga gggccgcggc gtggtggtct
     1801 acctggtcca ggagggccgg ggcatcgggc tgggcaacaa ggttcgcgcc tatcagctgc
     1861 aggacgacgg cgccgatacc gtcgacgcca acctccagct cgggttcgac gccgatctcc
     1921 ggtcgtacga tctggccgcc cggatcctcg ccgacctcgg ggtcgcctcc gtccgcctga
     1981 tgaccaacaa cccgcagaag atcagcggcc tggagcgcgc cggggtggtg gtcgccgcac
     2041 atgaaccgca ctgggtggcc gcgaccgagc acagcgcggg gtatcttgcg gcaaagaaga
     2101 ccaagctggg tcacctggtc taggtggtcc aggaggaaga cgatgatcac cgaagcagcc
     2161 gtcaagcagg cactgaccca ggtcatcgac ccggccctcg gccacgacat cgtcggctac
     2221 cggatggtgc gcagcgtcga ggtccagggc gacgacgcga tgatccggat cgacgtgccg
     2281 acccacgcct atccgctcgc caggcgccgc gagctggtcg tccagatcga gcaagcggtc
     2341 aaggcgctgg gcgccaagcg ggtcaccgtg atcccggagg tcgtcaccgc gttcctgccg
     2401 gcgccgagcg acaaggcgat gctcaagggc cccaagaacg tcatcgcggt ggcagccggc
     2461 aagggcggcg tcggcaagtc gacggtcgcg gtcaacctcg cgctcgcgct cgctcgccac
     2521 ggcgccaagg tcggcctgct cgacgccgac gtgttcgggc cgtcgatccc gaccatgctc
     2581 ggcgcgccgg aggtcccggc gtcggcgacc aaggactcgc ggatcacgcc agcggtgcac
     2641 cacggcctgc gcgggatcag cgtcggggtc ttcgtcgaca agggcgagac cgtggtgtgg
     2701 cgcgggccga tggtccatcg cctgctccag cagttcctgg gcgacgtcga ctggggcgag
     2761 ctcgactacc tggtgtgtga cctgccgccg ggcaccggcg acgtccagct ctcgctgtcg
     2821 cagctgatcc
//
LOCUS       DASVOR010000489         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_4597,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000489 DASVOR010000000
VERSION     DASVOR010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4597"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1520)
                     /locus_tag="VF516_09710"
     CDS             complement(<1..1520)
                     /locus_tag="VF516_09710"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX8107992.1"
                     /translation="MAWSRSSRPNRPLAAALALTIGPLAVWSAVRHLDGARRADAIAA
                     TAPQAVRPGYATSDRCEACHPAQYHSWHRGYHRTMTQYAGPASVRGDFADVTLSDGVD
                     EVHLSRRGDEFYAEMVDPLWQYQVDIGQRDAPPPGEPRPRVTRRISMVTGSHHMQAYW
                     VPGNEGNSQLSLPFTYLFDDHRWVPRSQVFLHPPEQDHDDEQVWNVSCIRCHTTAGQP
                     LALLPGGGWKIESKLGENGIACEACHGPAEAHVAANQNPLRRYWLHLRGAGDPTIVNP
                     ARLPAARGSEVCGQCHSLTDVSQDIELGEGKTYVAGAELESSQPLLRPLHPTPALERY
                     VAEDPLYLRNYFWADGTIRVSSRDYSGLVESKCMQSGRLWCGSCHSLHDSDPVNLLAR
                     GADADAACTSCHPRIAAAPEAHTHHAAASEGSRCASCHMPYTVYGLLKGIRNHRIDSP
                     RITDRAAALAGDERPNACNLCHVDRSLGWAAQQLARWYGQPVPARLAGGATPGDDTP"
BASE COUNT          205 a          626 c          690 g          267 t
ORIGIN      
        1 ggcgtgtcgt cgcccggggt ggcgccgccg gcgagccggg ccggcaccgg ctggccgtac
       61 cagcgcgcga gctgctgcgc cgcccagccc aggctgcgat cgacgtggca caggttgcag
      121 gcgttgggcc gctcgtcgcc ggccagcgcc gccgcgcgat cggtgatccg cgggctgtcg
      181 atccggtggt tgcggatccc cttgagcagg ccgtacacgg tgtacggcat gtggcagctg
      241 gcgcagcggc tgccctcgga cgcggcggcg tggtgggtgt gggcctcggg ggcggcggcg
      301 atccgcgggt ggcacgaggt gcacgcggca tcggcgtcgg cgccgcgcgc cagcaggttg
      361 accggatcgc tgtcgtgcag cgagtggcag ctgccgcacc acagccgtcc gctctgcatg
      421 cacttcgact cgaccaggcc gctgtagtcg cggctcgaca cccggatcgt gccgtcggcc
      481 cagaagtagt tgcgcaggta gagcggatcc tcggcgacgt agcgctccag cgccggcgtg
      541 gggtggagcg ggcggagcag cggctgcgag ctctccagct cggcgcccgc gacgtaggtc
      601 ttgccctcgc cgagctcgat gtcctggctg acgtcggtca gcgagtggca ctggccgcag
      661 acctccgagc cgcgcgcggc gggcagccgc gccgggttga cgatcgtcgg atcgccggcg
      721 ccgcgcaggt gcagccagta gcggcgcagc gggttctggt tggcggcgac gtgggcctcg
      781 gccgggccgt ggcaggcctc gcacgcgatg ccgttctcgc ccagcttgct ctcgatcttc
      841 cagccgccgc cgggcagcag ggcgagcggc tggccggccg tggtgtggca gcggatgcag
      901 ctgacgttcc acacctgctc gtcgtcgtgg tcctgctcgg gcgggtgcag gaacacctgg
      961 ctgcgcggga cccagcggtg gtcgtcgaac aggtaggtga acggcagcga cagctggctg
     1021 ttgccctcgt tgcccggcac ccagtacgcc tgcatgtggt gcgagccggt caccatcgag
     1081 atccggcggg tcacgcgcgg cctgggctcg ccgggcggcg gcgcgtcgcg ctggccgata
     1141 tcgacctggt actgccacag cggatcgacc atctcggcgt agaactcgtc gccgcgccgc
     1201 gacaggtgga cctcgtcgac gccgtcggac agcgtgacgt cggcgaagtc accacgcacc
     1261 gacgccgggc cggcgtactg ggtcatcgtg cggtggtagc cgcggtgcca cgagtggtac
     1321 tgcgccgggt gacaggcctc gcagcgatcc gaggtcgcgt agcccggccg caccgcctgg
     1381 ggcgcggtgg cggcgatcgc gtcggcgcgc cgggcgccgt cgaggtggcg caccgcggac
     1441 cacaccgcga gcggcccgat cgtcagcgcg agcgcagctg cgagcggccg gttcgggcgg
     1501 ctcgatcgcg accatgccac cggggaactg tatcacaggc cgatctccgg accgccgcgc
     1561 cgtcctggcg ccgtctgacc tcccttgact ccgtgccgcg ttcgcttacc ttcggcagcc
     1621 gatgtcggcg tccgatcgca gctggaccat tgcgccggta ctcctgtggt tctgcggctg
     1681 tctgatcgcc gcgctgttcg tgctcgacgg cggggtcgac gcgatcgccg accggttccg
     1741 cgcgcgccgc gagccggagc cggccggccc cgggttcggc gccggtcc
//
LOCUS       DASVOR010000490         3660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413057,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000490 DASVOR010000000
VERSION     DASVOR010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3660
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413057"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VF516_09715"
     CDS             <1..1519
                     /locus_tag="VF516_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107993.1"
                     /translation="TASQIHVSGGGPTGLVLDEDRGQLFVLTRFDDAISVIDTRTRAE
                     TAHVAMHNPEPPSITTGRRFLYDASFSSAHGDSACASCHVFGDFDSLAWDLGNPDAGV
                     IPNPGPFASALINLLVNPPQPITPEFHPMKGPMTTQSLRGMLNSGPMHWRGDRTGGND
                     APSAQPDSGTFDERAAFAKFQAGFTDLLGRDAFIPAADMDAFTDFILQVRYPPNPIRA
                     LDNSLTAEQEAGRQLFTATNCGIPAPDGNINGLTCISCHVIDPGGNPGTAAPGFFGTS
                     GLLSFDFEPQLLKTPHLRNLYQKVGMFGMPPTFGFGDTNTDFQGDQVRGFGFTHDGTL
                     DTVFRFHHALSFSDAFTGVGNGGFPAGPAGDPQRRQVEAFLLASPTDLAPIVGQQITL
                     AADSSASVAARVDLLRQRADAGDCDLVAKSTVAGAEAGFLYVGSGRFRTDRRALPPIG
                     DTALRLLAHLGHPVTFTCAPPGSGERIGVDRDGDGAWDGDERDAHTDPADPNSTP"
     gene            2384..>3660
                     /locus_tag="VF516_09720"
     CDS             2384..>3660
                     /locus_tag="VF516_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8107994.1"
                     /translation="MTSSSTSSSSSSSSSSSFTLFEAGQVRPLALSPDRQHLFATNTP
                     DNRLEIFRIEPHRLVHTGSIPVGLEPVAVAARSEREVWVVNHLSDSVSVVELDGDADS
                     GRVVRTLLVGDEPRDIVFAGPHRQRAFITTAHRGQNIPFDPMLTQAGIGRADVWVFDA
                     DALGDSLGGSPLTIVTLFTDTPRALAVTPDGSRVYAAGFHTGNQTTTVGQPVLPGFGQ
                     PEGQVGPTTNHAGVPAPDPGEIVQWNGTDFVNTAGTPRTDLVKFRLPDKDVFVLDAMA
                     NPPHPLSGPAGFFQHVGTILYSMVVNPVTGKVYVANTDANNTERFEGPGLFAGHSLRG
                     HLHESRITVLSPGGAVAPRHLNKHIDFHHCCAPVPNPENARSLARPQGMAVTRNGATL
                     YVAALGSDKVGVFSTAQLENDSFVPSTASQIHVS"
BASE COUNT          602 a         1444 c         1064 g          550 t
ORIGIN      
        1 caccgccagc cagatccacg tctccggcgg cggccccacc ggcctggtcc tcgacgagga
       61 ccgcggccag ctgttcgtgc tcacccggtt cgacgatgcg atctcggtga tcgacacccg
      121 gacccgcgcc gagaccgcgc atgtcgcgat gcacaacccc gagccgccca gcatcacgac
      181 cggccgccgg ttcctgtacg acgcatcgtt ctcgtcggcc cacggcgact cggcgtgcgc
      241 cagctgccat gtgttcggcg acttcgacag cctggcctgg gacctcggca accccgacgc
      301 gggggtgatc ccgaaccccg ggccgttcgc cagcgcgctg atcaacctgc tcgtcaaccc
      361 gccgcagccg atcacccccg agttccaccc gatgaagggg ccgatgacga cccagagcct
      421 gcgcggcatg ctcaacagcg gaccgatgca ctggcgcggc gaccgcaccg gcggtaacga
      481 cgcgccgagc gcgcagcccg acagcggcac gttcgacgag cgcgccgcgt tcgccaagtt
      541 ccaggccggg ttcaccgacc tgctcggccg cgacgcgttc atcccggccg ccgacatgga
      601 cgcgttcacc gacttcatcc tccaggtccg ctatccgccc aacccgatcc gcgcactcga
      661 caactcactc accgccgagc aggaagccgg ccgccagctg ttcaccgcca ccaactgcgg
      721 catcccggcg cccgacggaa acatcaatgg cctgacctgc atcagctgcc acgtgatcga
      781 ccccggtggc aaccccggca cggccgcgcc cgggttcttc ggcacgagcg ggctattgag
      841 cttcgacttc gaaccccagc tgctcaagac gccccacctg cgcaacctct accagaaggt
      901 cggcatgttc ggcatgcccc cgaccttcgg cttcggtgac accaacaccg acttccaggg
      961 cgatcaggtc cgcggcttcg gcttcaccca cgacggcacg ctcgacaccg tgttccggtt
     1021 ccaccacgcg ctcagcttct cggacgcgtt caccggcgtc ggcaacggcg ggttccccgc
     1081 cggccccgcg ggcgatcccc agcgccggca ggtcgaggcg ttcttgctcg cgtccccgac
     1141 cgacctcgcg ccgatcgtcg gccagcagat cacgctcgcc gcggacagct cggcctccgt
     1201 cgccgcccgc gtcgatctgc tccgtcagcg cgccgacgcc ggcgactgcg atctggtcgc
     1261 caagtccacc gtggccggcg ccgaggcggg cttcctctac gtcggcagcg gccggttccg
     1321 caccgaccgc cgcgccctgc ctccgatcgg cgacaccgcg ctgcgcttgc tcgcccacct
     1381 cggccacccg gtgaccttca cctgcgcccc acccggctcc ggcgagcgga tcggcgttga
     1441 ccgcgacggc gacggcgcct gggacggcga cgagcgcgac gcccacaccg atcctgccga
     1501 cccgaacagc acgccctgac cggagccgca gctccgggat gtcgcggtcg tagacccgcc
     1561 agcaggtgac gccctcgcgc cggcccgccg ctggctaggt gagccagcgg acgcgtagct
     1621 tcacactgcg taatatgttc cgatccgtgg ggtacgactg aggtagcccg ttgcctcagc
     1681 agcccgaact gcaataatcg atcggatcaa gccgcgtcga acgaacgttt cacacccgag
     1741 aatgggacct cgcacctcac gatcacgagg ggcaggtctg aactgacagg caggatgtgg
     1801 cagatcccac gcggcggagc agccacacct cacccgggtg taacattgct gttggcgcgt
     1861 tcgcggtaat ccgcagatcg tgtcagcccg ccacgatcgt cataaatctg ggaccacaag
     1921 ccccatcgcc gcatcgcgat cgtcataaat ctgcggccga gatcgtgtga gctcaccacg
     1981 atcatctggc gactggacta ttcgttctac acaacaccca ccgctgactc tgattcggcg
     2041 cagcgcccaa atgaaaacta tgttgcccgg atacttgtga gttcatcctt gacaaaagaa
     2101 ctacaggctt ccaaggtaat cttgagtttt caagacccac gatcgagtca tgcagtcctc
     2161 ctgggtcgat aacgtgcaca actcttttca atgggggaat gaaaacatgg attcgcgaca
     2221 tgcacatggt ggccggcagt ggccgcccac cgctgtcacg gcgatctgcc tcggtctggc
     2281 ggcggcgccg tcctgcgcgc cacctggcga ggccggtcgc tccgaccagc ctgtcgaggt
     2341 cgacggcacg gcatcccgtc cggccgcgcc ggcccgatcg gcgatgacga gctcgtcgac
     2401 gagctcgtcg tcgagctcat cgtcgagctc atcgttcacg ctgttcgagg ccggccaggt
     2461 ccgtccgctc gcgctgtcgc ccgaccggca gcacctgttc gcgaccaaca ctccggacaa
     2521 ccgcctcgag atcttccgga tcgagcccca ccgcctggtc cacaccgggt cgatcccggt
     2581 cgggctcgag ccggtcgccg tcgccgcgcg ctccgagcgt gaagtctggg tcgtcaacca
     2641 cctgtccgac agcgtcagcg tggtcgagct cgacggcgac gccgactccg gccgcgtcgt
     2701 ccgcaccctg ctggtcggcg acgagcctcg cgacatcgtg ttcgccgggc cgcatcgcca
     2761 gcgcgcgttc atcaccaccg cccaccgcgg ccagaacatc ccgttcgatc ccatgctcac
     2821 ccaggcgggc atcggccgcg ccgacgtctg ggtgttcgac gccgatgcgc tcggcgactc
     2881 gctcggcggc tccccgctca cgatcgtcac cctgttcacc gacaccccgc gcgccctcgc
     2941 ggtcaccccc gacggctccc gggtctacgc cgccggcttc cacaccggca accagaccac
     3001 caccgtaggc cagccggtgc taccggggtt cgggcagccc gagggtcagg tcggccccac
     3061 caccaaccac gccggcgtcc cggcgcccga ccccggcgag atcgtccagt ggaacggcac
     3121 cgacttcgtc aacaccgccg gtactccacg caccgatctc gtgaagttca ggctgcccga
     3181 caaggacgtg ttcgtgctcg acgcgatggc caacccaccg catccgctgt ccggccccgc
     3241 cggcttcttc cagcacgtcg gcaccatcct gtactccatg gtcgtcaacc cggtcaccgg
     3301 caaggtctac gtcgccaaca ccgacgccaa caacaccgag cgcttcgagg gtcccggtct
     3361 gttcgccggc cacagcctgc gcggccacct ccacgagagc cggatcaccg tgctgtcccc
     3421 gggaggtgcc gtcgcgcccc gccacctcaa caagcacatc gacttccacc actgctgcgc
     3481 gcccgtcccc aaccccgaga acgcaaggtc cctcgcccgg ccccagggca tggccgtcac
     3541 ccgcaacggc gccacgctct acgtcgccgc cctgggctcc gacaaggtcg gcgtgttctc
     3601 caccgcccag ctcgagaacg acagcttcgt gcccagcacc gccagccaga tccacgtctc
//
LOCUS       DASVOR010000491         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_355241,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000491 DASVOR010000000
VERSION     DASVOR010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355241"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..376
                     /locus_tag="VF516_09725"
     CDS             <1..376
                     /locus_tag="VF516_09725"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107995.1"
                     /translation="LSLGLFATGEFGAPTPTGPDTSAGALVTGLFYGGGWSQLKVQAI
                     GSAAVTIAVLAVSLALMYGVKAVGWLRVSTEGELEGLDKHEHGGPAYPELVGAVHSGE
                     PVDEATGLGAASSAVAMSATGP"
     gene            789..>1816
                     /locus_tag="VF516_09730"
     CDS             789..>1816
                     /locus_tag="VF516_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107996.1"
                     /translation="MTSSNPPRIAIRNLSFTTLAVIAWCATPAAAADGDAKAAVASAA
                     DGKTTVATAAAPEATTPPDPRDPAAGATTTATAKSEPAAPTVRYPRAVIERPLTLPAG
                     VAMLGADLTSNNDMTAMGGAPIIGYGITDDLEIQVPYAFATREFEAKGSLDFDVGYKL
                     LRGAAGGKLEVIARARTGYNFLTRAANPLMVGVHVQYNITDKLAVISGFPSTQQLSIA
                     LSDGGVMGAPRPIDLALPLSAGYQATPLLYFQLDTRLARLGLSNSSSAWIGADTTPVA
                     LTAVYNVAHALDVQASIGTDLSSSPGDALSFLVGARYYAGQLGAPPARTIRRAGAPAR
                     AASAPARA"
BASE COUNT          266 a          669 c          621 g          260 t
ORIGIN      
        1 gctcagcctc gggctgttcg ccacgggcga gttcggcgcg cctacgccga ccggcccgga
       61 tacctcggcg ggagcgctgg tgaccgggct gttctacggt ggcggctggt ctcagctcaa
      121 ggtccaggcg atcggcagcg cggcggtcac catcgcggtg ctcgcggtgt cgctggcgct
      181 gatgtacggg gtcaaggcgg tcggctggct gcgggtgtcg accgagggcg agctcgaggg
      241 cctcgacaag cacgagcacg gcggcccggc gtaccccgag ctggtcggcg cggtacacag
      301 cggcgagccg gtcgatgagg ccacggggct cggggcggcg agctcggccg tggcgatgtc
      361 ggcgaccggc ccctgaccgg ccgcctgtgc tccttccgcg atgacgaccg cggcgcggcc
      421 ccgcgccgcc gggtccggcg accggccgcg ggtacctgat gatcgcgctc ggcggctaca
      481 tcccatcgcc gctcgctcgc ctggtgtggg gttcggtcac ccacgaggtc gtcgagcgcg
      541 cgactgttcc ggtgtccctg cactactgat ctgccaccca gatcggcatc gtaggggcca
      601 cccctgtctc cggatcacgg gagtgactcc ggggcacatc attgattgcg attcgatgtt
      661 tccgctgcgt atcgccacga tgacggcgaa gctcgtcgag agccctcgac gacctcttga
      721 tgagatttta tcgaagagat acgatccggc aatcggacag aaatgtctat cggccgacag
      781 tcgagctcat gacgagctcg aatccaccgc gtattgcgat cagaaatctc tcgttcacca
      841 cactggccgt gatcgcgtgg tgcgcgacgc cggccgccgc cgccgacggc gacgccaagg
      901 ccgcggtcgc cagcgccgcc gacggcaaga ccacggtcgc gaccgccgcc gcgcccgagg
      961 ccacgacgcc gcccgatccc cgcgatcccg ccgccggcgc gacgaccacc gccaccgcga
     1021 agagcgagcc ggccgcgccg acggtgcgct atccgcgcgc cgtgatcgag cggccgctga
     1081 ccctgcccgc cggcgtggcg atgctcggcg ccgacctgac ctccaacaac gacatgaccg
     1141 cgatgggcgg cgcgccgatc atcggctacg gcatcaccga cgatctcgag atccaggtgc
     1201 cgtacgcgtt cgcgacccgc gagttcgagg ccaagggcag cctcgacttc gacgtcggct
     1261 acaagctgct gcgcggcgcg gcgggcggca agctcgaggt gatcgcgcgc gcgcgcaccg
     1321 gctacaactt cctcaccagg gccgccaacc cgctgatggt cggcgtccac gtccagtaca
     1381 acatcaccga caagctggcg gtgatctcgg ggttcccgag cacccagcag ctcagcatcg
     1441 cgctgtcgga cggcggtgtc atgggcgcgc ccaggccgat cgacctggcg ctgccgctca
     1501 gcgcgggcta ccaggccacg ccgctcctgt acttccagct cgacaccagg ctcgcccggc
     1561 tgggcctcag caactcgtcc agcgcgtgga tcggcgccga cacgaccccg gtcgcgctga
     1621 ccgcggtcta caacgtcgcc cacgcgctcg acgtccaggc ctcgatcggc accgacctgt
     1681 cgagctcgcc gggcgacgcc ctgagcttcc tggtcggggc gcgctactat gccggccagc
     1741 tgggagcgcc gccggcgcgc actatccgcc gggctggagc tcctgcaagg gcggcatccg
     1801 cgccagcgcg cgccag
//
LOCUS       DASVOR010000492         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413075,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000492 DASVOR010000000
VERSION     DASVOR010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413075"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF516_09735"
     CDS             <1..211
                     /locus_tag="VF516_09735"
                     /inference="COORDINATES: protein motif:HMM:NF015114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8107997.1"
                     /translation="LGNTGDPRAIPALIGLLRHPAAVVRGHAVWALGELGAAEVLAGY
                     EDSDPAVADELTWARRRSQRAPQDP"
     gene            complement(165..548)
                     /locus_tag="VF516_09740"
     CDS             complement(165..548)
                     /locus_tag="VF516_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8107998.1"
                     /translation="MKSWLLAVVVVVAVPGGVACNRDPAGGASDGATVYGMLCATCHG
                     PDGRPPAAMVARLGVRDLTAPEFRARVAASGPGLVEQQVRHGSQNKLMPAFEGAISDA
                     QIRAVAAYVASTGLGGLAGSGAAPR"
     gene            605..1486
                     /locus_tag="VF516_09745"
     CDS             605..1486
                     /locus_tag="VF516_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8107999.1"
                     /translation="MRWIVSLSIVLVCAVTACGGPQIPTHNGYKPKEATPWKKSKALK
                     FDDKMEAKAEGDLSYADMRRARWFELNLPANGQLTLGLEITPPGDAVNDDFDLGMEVI
                     DPSYRVISKSDLEDEDAHELNKKKTLVELAPGKYLIHLFLQGRMDTADFVLHAAFKPT
                     AAAEVKSNFPAEVPFVPVLAQVPIQDDTPRNYKPVVVEKHGARHPRPAAPTPAAAPVA
                     TVSARIINVQIVAGGTQITLGRGTTTGAADGMHGSIHGVPGGFQIGGCNERTCTATIK
                     ATPDQIKASDGTVTLTP"
     gene            1622..2686
                     /locus_tag="VF516_09750"
     CDS             1622..2686
                     /locus_tag="VF516_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108000.1"
                     /translation="MVGSRGCLAAILVLASSCAHNVPQDSNTGADGKEKGAKPITMEN
                     GEGKASGIVTYPGGDRVDWKLVELPDKQHGTLDIQLAWAPPRSGLQLAFDVFDEWNRP
                     IVQSQKSSKKAKGRTRSATVEDARGKYLIRVYAVGRGDAGKYRLTVDFKEATSGPGFD
                     PLKLDIPDPPKLAAVPDVVPPCDPLQFDPKNPECKLVCPDVGAPPGWPACKDKCPTPP
                     NVELPACQKTMDCPNPPDRRVLKCIAEKERKWPKCPDPRNPDPNNPNCDNIKIPPVVG
                     RIIGSSIQGNEVIITIGAGSNSGIAPNWTAHVLSGSSDNPLAGGDIKVVRVDKQVTVG
                     KTKLTPDQLLSNPRVKLSAP"
     gene            2683..>3369
                     /locus_tag="VF516_09755"
     CDS             2683..>3369
                     /locus_tag="VF516_09755"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108001.1"
                     /translation="MNADPFVGKVIDGRYEIQTRIGEGGMGVVYKARQTPLDRVIALK
                     MLNSQMATDPTWVQRFYNEAKACSRLQHPNTIRMFDFGQTPDGRLFMTMEFLDGVSLR
                     EALSRGPLAAHRVVKILIQCCASLAEAHSIGIIHRDIKPDNVFLLNMAGSPDFVKLLD
                     FSVAKLLEGDRMKTQAGVVFGTPQYMSPEQGRGMPLDARSDLYALGILAFEMLTGNVP
                     FHDENPMTVIQ"
BASE COUNT          596 a         1188 c         1138 g          447 t
ORIGIN      
        1 gctcggcaac accggcgatc cgcgggcgat cccggcgctg atcggcttgc tccgccaccc
       61 cgcggcggtg gtccgcggcc acgcggtgtg ggcgctcggc gagctcgggg cggcggaggt
      121 gctggcgggg tacgaggaca gcgacccggc ggtggccgac gagctcacct gggcgcggcg
      181 ccggagccag cgagcccccc aagacccgta gacgcgacat acgccgccac cgcccggatc
      241 tgcgcatcgc tgatcgcgcc ctcgaacgcc ggcatcagct tgttctgcga cccgtggcgg
      301 acctgctgct cgaccaggcc cggcccggac gcagcgaccc gggcgcggaa ctccggcgcg
      361 gtcagatctc ggacgccgag ccgggcaacc atggccgccg gcggccggcc gtcggggccg
      421 tgacaggtcg cgcacagcat gccgtagacg gtggccccgt cgctggcccc acccgccgga
      481 tcgcggttgc aggcaacgcc gcccggcacc gcgacgacca cgaccacggc aagcagccac
      541 gacttcatgc gagcgatgtg ccactgacca cagcggccgg caaccccatg gtagtgttcg
      601 cgctatgcgg tggattgtct cgctgtcgat cgtcctggtg tgcgccgtga cggcctgtgg
      661 tggtccgcag atcccgaccc acaacggcta caagccgaag gaagccacgc cctggaagaa
      721 gtccaaggcg ctcaagttcg acgacaagat ggaggccaag gccgagggtg acctgtcgta
      781 cgccgacatg cggcgcgcgc ggtggttcga gctcaacctg ccggccaacg gtcagctgac
      841 gctcgggctg gagatcacgc cgcccggcga cgcggtcaac gacgacttcg acctcgggat
      901 ggaggtgatc gacccgagct accgcgtgat cagcaagtcg gatctcgagg acgaggacgc
      961 gcacgagctc aacaagaaga agacgctcgt cgagctcgct cccggcaagt acctgatcca
     1021 cctcttcctg cagggccgga tggacaccgc cgacttcgtg ctgcatgccg cgttcaagcc
     1081 gaccgccgcg gccgaggtca agagcaactt ccccgccgag gtgccgttcg tgccggtgct
     1141 ggcgcaggtc ccgatccagg acgacacgcc gcgcaactac aagccggtgg tcgtcgagaa
     1201 acacggggcc cggcatccgc ggccggcggc gccgaccccg gcggcggcgc cggtcgcgac
     1261 ggtcagcgcg cggatcatca acgtccagat cgtcgcgggc gggacccaga tcaccctggg
     1321 gcgcggcacg acgaccggcg cggccgacgg catgcatggc tcgattcacg gcgtaccggg
     1381 cgggttccag atcgggggct gcaacgagcg cacgtgcacg gcgacgatca aggccacgcc
     1441 ggatcagatc aaggcatcgg acggcacggt gacgctcacc ccgtagcacc cggccgtagc
     1501 acccggccgt agcgcccggc cgtagcgcct ggccgtagcg ccccgtcgcg ctgccgcggc
     1561 gtcgcacaag cccttgcgat cacgcgtcaa ttagcgtaac acgagggcta tgctcgggaa
     1621 cttggtgggt tcgaggggct gtctcgcagc gatcctcgtg ctcgcgtcgt cgtgtgctca
     1681 caacgttccg caggactcca acaccggcgc cgacggcaag gagaagggag ccaagccgat
     1741 cacgatggag aacggcgagg gcaaggcgag cggcatcgtg acgtatcccg gcggcgatcg
     1801 cgtcgactgg aagctcgtcg agctgccgga caagcagcac ggcacgctcg acatccagct
     1861 cgcgtgggcg ccgccgcggt ccgggctgca gctcgcgttc gacgtgttcg acgagtggaa
     1921 ccgaccgatc gtccagtcgc agaagagcag caagaaggcc aagggccgca cgcggtcggc
     1981 gaccgtcgag gacgccaggg gcaagtacct gatccgggtc tacgcggtcg gccgcggcga
     2041 cgccggcaag taccggctca ccgtcgactt caaggaggcg acctccggcc cggggttcga
     2101 cccgctcaag ctcgacatcc cggatccccc caagctcgcc gcggtccccg acgtcgtgcc
     2161 gccgtgcgat ccgctccagt tcgacccgaa gaacccggag tgcaagctgg tgtgtcccga
     2221 cgtcggcgcg cctccgggct ggccggcgtg caaggacaag tgccccaccc cgccgaacgt
     2281 cgagctcccc gcgtgccaga agacgatgga ctgccccaac ccgcccgacc ggcgggtgct
     2341 caagtgcatc gccgagaagg agcgcaagtg gcccaagtgc cccgatccga ggaatccgga
     2401 tccgaacaac ccgaactgcg acaacatcaa gatcccgccc gtggtcggcc gcatcatcgg
     2461 cagctcgatc cagggcaacg aggtcatcat cacgatcggc gccggcagca actccgggat
     2521 cgcgccgaac tggacggcgc acgtgctgag cggcagcagc gataacccgc tcgcgggcgg
     2581 cgatatcaag gtcgtccgcg tcgacaagca ggtgacggtg gggaagacca agctcacccc
     2641 ggaccagctg ctgtccaatc cccgcgtcaa gctgagcgca ccgtgaacgc ggatcccttc
     2701 gtcggaaagg tcatcgacgg ccgctacgag atccagaccc ggatcggcga gggtggcatg
     2761 ggcgtggtct acaaggcgcg gcagacgccg ctcgaccgcg tgatcgcgct gaagatgctg
     2821 aactcgcaga tggcgaccga cccgacctgg gtccagcgct tctacaacga ggccaaggcg
     2881 tgctcgcgcc tgcagcaccc caacaccatc cgcatgttcg acttcggcca gactcccgac
     2941 ggccggttgt tcatgacgat ggagttcctc gacggagtct cgctgcgcga ggcgctgtcc
     3001 agggggccgc tggcggcgca ccgcgtggtc aagatcctga tccagtgctg cgcctcgctc
     3061 gccgaggccc actcgatcgg catcatccac cgcgacatca agcccgacaa cgtgttcctg
     3121 ctcaacatgg cgggctcgcc ggacttcgtg aagctgctcg acttctcggt cgccaagctg
     3181 ctcgagggcg accggatgaa gacccaggcc ggcgtcgtgt tcggaacgcc gcagtacatg
     3241 tcgcccgagc agggccgcgg catgccgctc gacgcgcgct cggacctcta cgcgctcggc
     3301 atcctcgcgt tcgagatgct gaccggcaac gtgccgttcc acgacgagaa cccgatgacg
     3361 gtgatccag
//
LOCUS       DASVOR010000493         3898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_180028,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000493 DASVOR010000000
VERSION     DASVOR010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180028"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1749
                     /gene="fusA"
                     /locus_tag="VF516_09760"
     CDS             <1..1749
                     /gene="fusA"
                     /locus_tag="VF516_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8108002.1"
                     /translation="QSQSYTVDRQMRRYKVPRIAFVNKLDRTGANPYRVRDQLRDKLK
                     LHPVMIQMPIGLEDKLEGVIDLIEMVAFKFYGDNGETIERGPIPPELMEQAEKLRHEM
                     LDNLSLVSDELTEAILEDKVTPELIRKSLREATIALKCTPVMCGSALGNKGVQLLLDG
                     VCAYLPEPRDVENVALDLDKAEEPVVLESNPDKPLVMLAFKLEDGRYGQLTYVRVYQG
                     RVRKDDFIVNSRTGKKVKVGRLVRMHSDDKEEITDAGAGDICAMFGVDCNSGDTFTDG
                     TVNFAMTSMFVPATVIEVAIKPKDSGAEINMSKALNRFTKEDPTFKCWVDPESNETII
                     AGMGELHLEVYIERMKREYKAEVITSPPQVAYRETIGARVDFSYTHKKQTGGSGQYGK
                     VAGFAEPFEGDFEFVDEIRGGAIPREFIPSCEKGFRSMLAKSPRLGVPVVGLRVAIND
                     GQSHAVDSSDIAFQEAARGAFREFFPRARPKILEPIMKLSVEGPAEFSGNILSTVMQR
                     RGVVIGSTEEDGMCRVDSEVPLAEMFGYSTTLRSSTQGKAEFTMEFAKYAETPSNISE
                     ELLAKAAKAKQEAAKK"
     gene            1808..2863
                     /locus_tag="VF516_09765"
     CDS             1808..2863
                     /locus_tag="VF516_09765"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HEX8108003.1"
                     /translation="MRHAAGSLALLLVTTTALAAPPASPSAGADPSALSERTTLLAPI
                     QVDSLTLTPIIATGPTDPTGDQLLVLDEAMAARQVTIKEISDGSVNSLTFVNKAPQPV
                     FLLAGEVIIGGKQDRIIGRNTVIPANTTQEVPVYCVEHGRWTVQTKEFASARALAHGR
                     LRGQASYAAQQDVWNEVAAKNVARKTVTPSDTYRRVAQQQSDGTLADMEKRVNAALAK
                     LPPADRGRMVGYAVALNGSVATVDVFGSPALFKKLETKLVRSYLTEAVDVRARPDTRP
                     PTATDVKAFMADADKAAESKSYDTGAADTMVNRGEKAFKAKVGYKRRPAAKADGQPAP
                     AAAAPAEPTVYENYQAK"
     gene            3099..3734
                     /locus_tag="VF516_09770"
     CDS             3099..3734
                     /locus_tag="VF516_09770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8108004.1"
                     /translation="MVTTVRALMKNAVYDAFPGVRRRGPNTSRRVALTFDDGPDHMTE
                     RYLDMLDELGVPATFFLVGGRAASRPDLVREYRRRGHQIAGHGYDHKRFSKLGRRELL
                     EQCARTESALGGQLSGRPWVRPPHGSLDASSLVYLVAAGYTVAMWSLDSRDYGTTNPD
                     DLVHECAGMSAGDVVLFHEGQQWTLDALPRIVADLHAAGLECVTMHDLFAT"
BASE COUNT          659 a         1376 c         1330 g          533 t
ORIGIN      
        1 cagtcgcaga gctacaccgt cgaccgccag atgcggcgct acaaggtgcc gcgcatcgcg
       61 ttcgtcaaca agctcgaccg caccggcgcc aacccgtacc gcgtgcgcga ccagctgcgc
      121 gacaagctca agctccaccc ggtgatgatc cagatgccga tcggcctcga ggacaagctc
      181 gagggcgtga tcgatctgat cgagatggtc gcgttcaagt tttacggcga caacggcgag
      241 accatcgagc gcggcccgat cccgcccgag ctcatggagc aggccgagaa gctgcgccac
      301 gagatgctcg acaacctgtc gctggtgtcc gacgagctga ccgaggcgat actcgaggac
      361 aaggtcaccc cggagctgat ccgtaagtcc ctgcgcgagg cgacgatcgc gctcaagtgc
      421 acgccggtga tgtgcggctc ggcgctcggc aacaagggcg tgcagctctt gctcgacggc
      481 gtgtgcgcgt acctgcccga gccgcgcgac gtcgagaacg tggcgctcga cctcgacaag
      541 gccgaggagc cggtggtcct ggagtccaac ccggacaagc cgctggtcat gctcgcgttc
      601 aagctcgagg acggccgcta cggccagctg acctacgtcc gggtctacca gggcagggtc
      661 cgcaaggacg acttcatcgt caactcgcgc accggcaaga aggtcaaggt cggccggctg
      721 gtccggatgc actccgacga caaggaggag atcaccgacg ccggcgccgg cgacatctgc
      781 gcgatgttcg gcgtcgactg caactcgggc gacacgttca ccgacggcac cgtcaacttc
      841 gcgatgacgt cgatgttcgt gccggcgacc gtgatcgagg tcgcgatcaa gcccaaggac
      901 tcgggcgccg agatcaacat gtccaaggcg ctcaaccggt tcaccaagga ggaccccacg
      961 ttcaagtgct gggtcgatcc ggaatcgaac gagaccatca tcgccggcat gggtgagctc
     1021 cacctcgagg tctacatcga gcgcatgaag cgcgagtaca aggccgaggt catcacctcg
     1081 ccgccccagg tcgcgtaccg cgagacgatc ggcgccaggg tcgacttcag ctacacccac
     1141 aagaagcaga ccggcggctc gggccagtac ggcaaggtcg caggcttcgc cgagccgttc
     1201 gagggcgact tcgagttcgt cgacgagatc cgcggcggcg cgatcccgcg cgagttcatc
     1261 ccgtcgtgcg agaagggctt ccggtccatg ctcgccaaga gcccgcggct cggcgtgccg
     1321 gtggttggcc tccgggtcgc gatcaacgat ggccagtccc acgcggtcga ctcgagcgac
     1381 atcgcgttcc aggaagccgc gcgcggtgcg ttccgcgagt tcttcccgcg cgcccggccc
     1441 aagatcctcg agccgatcat gaagctgtcg gtcgagggcc cggccgagtt ctcgggcaac
     1501 atcctgtcga cggtgatgca gcgccgcggc gtggtgatcg gctcgaccga ggaggacggc
     1561 atgtgccggg tcgactccga ggtgccgctc gccgagatgt tcggctactc gaccacgctg
     1621 cgcagctcga cccagggcaa ggccgagttc accatggagt tcgccaagta cgccgagacg
     1681 cccagcaaca tctcggagga gctgctggcc aaggccgcga aggccaagca ggaagccgcc
     1741 aagaagtagc ggtcaaaagt cctagatcgg cgtgggcggc ggcgcgttgg gaccgtgcgg
     1801 aggtcccatg cgccacgctg ccggttccct tgcgctcttg ctcgtcacca ccaccgccct
     1861 cgccgctccg cccgcctcgc catccgccgg cgcagatccg tcggcgctgt ccgagcgcac
     1921 cacgctgctc gcgccgatcc aggtcgacag cctgacgctg acgccgatca tcgccaccgg
     1981 cccgaccgac ccgaccgggg atcagctgct cgtgctcgac gaggcgatgg ccgcccggca
     2041 ggtcacgatc aaggagatct cggacggcag cgtcaacagc ctgaccttcg tcaacaaggc
     2101 gccgcagccg gtgttcctgc tcgccggcga ggtcatcatc ggcggcaagc aggaccgcat
     2161 catcggccgc aacaccgtga tcccggcgaa caccacgcag gaggtgccgg tgtactgcgt
     2221 cgagcacggg cggtggaccg tccagaccaa ggagttcgcc agcgcgcggg cgctcgccca
     2281 cggccggctg cgcggccagg ccagctacgc cgcccagcag gacgtatgga acgaggtcgc
     2341 cgccaagaac gtcgcgcgca agaccgtcac gccttcggac acctaccgca gggtcgcaca
     2401 gcagcagtcc gacgggacgc tggcggacat ggagaagcgg gtcaacgccg cgctcgccaa
     2461 gctcccgccg gcggaccgcg gccggatggt cggctacgcg gtcgcgctca acggctcggt
     2521 cgcgaccgtc gacgtgttcg gctcgcccgc gctgttcaag aagctggaga ccaagctggt
     2581 gcggtcgtac ctgaccgagg cggtcgacgt ccgcgcccgg ccggacacca ggccgccgac
     2641 cgccaccgac gtcaaggcgt tcatggccga tgccgacaag gccgccgaga gcaagtcgta
     2701 cgacaccggc gcggccgaca cgatggtcaa ccgcggcgag aaggcgttca aggccaaggt
     2761 cgggtacaag cggcgccccg ccgcgaaggc cgacggccag ccggcgcctg cggcggcggc
     2821 gccggccgag ccgacggtgt acgagaacta ccaggcgaag tgacctcgct ggccggttcc
     2881 aaagcgctgg ctgcccgacg cgtttcgcgg cgaagtgacc caccgacgcc gcacgaagcg
     2941 gcaccggtgc gaggtcatcc gctccggccg ctccggcgcg ccggcgcggt gtgcggcgca
     3001 gcgcggtgcg acgctccggc acggatcgcg cgcggttctc acgaactcga tcccggtgcc
     3061 cgcttgtcct gtcgcgtgag agaccgtatg aacaagatgt ggttactacc gtacgcgccc
     3121 tgatgaagaa cgcggtctac gatgcgttcc cgggcgtgcg ccgccggggt ccgaacacgt
     3181 cccggcgcgt cgcgctgacg ttcgacgacg gaccggacca catgaccgag cgctacctcg
     3241 acatgctcga cgagctcggg gtccccgcga cgttcttcct ggtcggcgga cgagccgcga
     3301 gccggcccga cctcgtgcgc gagtatcgcc ggcgcggcca ccagatcgcc ggccacggct
     3361 acgaccacaa gcggttcagc aagctcggcc gccgcgagct gctcgagcag tgcgcgcgca
     3421 ccgagtccgc gctcggcggc cagctcagcg gccggccgtg ggtccgtccc ccacatgggt
     3481 cgctcgatgc gagctcgctg gtctacctgg tcgcggccgg ctacacggtc gcgatgtggt
     3541 cgctcgactc ccgcgactac ggcacgacca acccggacga cctcgtccac gagtgcgccg
     3601 ggatgagcgc gggggacgtc gtgctgttcc acgagggcca gcagtggacc ctggacgcgc
     3661 tgccgcgcat cgtcgccgac ctccacgccg cgggcctgga gtgtgtgacc atgcacgacc
     3721 tgttcgcgac ctgagccggg ctggcgcccg gtgatcgatc gccgggagcg aggcggtgcg
     3781 ccccgcggac ggccagccgg gctggttggt ccctcgcgcc ggtctgcgcc ccgcagatcc
     3841 tgatccgcgg ctcgcgggcg gcgccgcggc cggttaccag ccgctcgcgg gcggcgct
//
LOCUS       DASVOR010000494         2304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413091,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000494 DASVOR010000000
VERSION     DASVOR010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413091"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..1059)
                     /locus_tag="VF516_09775"
     CDS             complement(148..1059)
                     /locus_tag="VF516_09775"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEX8108005.1"
                     /translation="MDEVSPRVTAIELAQLGERLSPLRLRSPEALREMERSLSRHGQL
                     VAVVCHRQDDQVEVVDGFKRLIGARALGWPVLRVEIHEVTGPAAKLLLWQSNARQALS
                     DLEEAWLVRALYRDDRLTQPQIGQLLGRHKSWVCRRLVLAEGLTESVTADIRLGLVSA
                     TAAREVGRLPRGNQEQVAQVIARRGLTTRQTTRLVEQLLAASDERGRAAVLAQAERTS
                     APPAPRPVRAPVTPGEAMIADADALATRATRLHTRLLERPVTSLGVEVERVVVGRLGE
                     LQAVLAALCQTLGQVTSTSAAGVSHGA"
     gene            complement(2114..>2304)
                     /locus_tag="VF516_09780"
     CDS             complement(2114..>2304)
                     /locus_tag="VF516_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108006.1"
                     /translation="PTPQVAFRLPDSLIARLDRHVDRMTTEHPGLDFTRADAVRSLLT
                     RALDQIEGSSAPTKRGGR"
BASE COUNT          332 a          919 c          694 g          359 t
ORIGIN      
        1 ggcgccaccg gcccgctccg cgccgccgcg tgcgcgcgca ggatcttgcg gacccggttg
       61 cgccccacgt gcagcgcccg ggcgatcgcg cgatcgctca tctgctgctg cgcaagcatc
      121 accacgtcgt gcgccagctc ctcgggacta cgcgccatgg ctcacccccg ctgcgctcgt
      181 cgaggtcacc tggcccagcg tctggcacag cgccgccagg accgcctgca gctcgcccag
      241 tcggccgacc accacccgct ccacctcgac gcccaggctc gtcacgggtc gctccagcag
      301 ccgggtgtgc aaccgggtcg cgcgcgtcgc cagcgcgtcg gcgtcggcga tcatcgcctc
      361 gcccggcgtc accggcgcgc gcactggtcg cggtgcgggc ggcgcactgg tccgctcggc
      421 ctgggccagg accgccgcgc gcccgcgctc gtcgctggcg gccagcagtt gctcgaccag
      481 ccgggtggtc tgtcgcgtgg tcagcccgcg ccgcgcgatc acctgcgcga cctgctcctg
      541 gttgccgcgc ggcaaccggc cgacctcccg cgccgccgtc gccgagacca gcccgagccg
      601 gatgtccgcg gtcacgctct cggtcagccc ctccgcgagc accagccggc gacacaccca
      661 gctcttgtgg cgccccaata gctgcccgat ctgcggctgc gtcagccgat cgtcgcggta
      721 cagcgcacgg accagccacg cctcctcgag gtctgacagc gcttgccgcg cgttgctctg
      781 ccacagcagc agcttggccg cgggcccggt gacctcatgg atctcgacgc gcaggaccgg
      841 ccagccgagc gcgcgggccc cgatcaatcg cttgaagccg tcgacgacct cgacctggtc
      901 gtcctggcgg tgacacacca ccgcgaccag ctggccgtgt cgcgacagcg accgctccat
      961 ctcgcgcagc gcctcgggcg agcgcagccg caacggcgac agccgctccc ccagctgcgc
     1021 gagctcgatc gcggtgaccc tcggcgagac ctcgtccatg gcgccaccct ccgcgaatcg
     1081 ccgtccacag tcgacccggt gcgtgacatc gctaccaagc ctttcggatg tttcactagt
     1141 cctttcatga agctaaaaat ccgacccggt gcgtgacagg cgtcgacgtc aaccacgaaa
     1201 accgccactt tccttggtcc aattcgcacg ttgcggcgtc gacccggtgc gtgacagctg
     1261 tatcgtccac cgcccaaggc ttgccagcct tttctggatt tcctccacca tatcaagcac
     1321 ctccgcctcg ctcctggtgc gtgacatccg gacacggcct ggcgcggcca cgggctcctt
     1381 gccggcacga tgcttggctt gccgccgtcg ctcgtcggcg cgtgactgcg ccagatcgtt
     1441 cctccgtcga gcccgcacta accggttgtg atgcctgcgt cgacactcga tgcgtccaca
     1501 caaccgctgc cccgcccggc acggccgaaa tgagcaacga cactcgacgc atcgcttcgt
     1561 gatcgacacc caaccgtctt gcacgacggc tccgtcggca cgacgcccac accctcgcga
     1621 tcatcgccgc cggatccatc ccaccgccgg atcgccgccg ccggatccat cccaccgctg
     1681 gatcaccgcc gccggatcca tcccaccgcc gtcggatcca ccaccaccgt cggatccacc
     1741 accgccaccg gatccaccac cgccgccgga tccaccaccg ccgtcacgat gcggcggtca
     1801 gctggctccc gtggatccac tcgccgccac aaggtggctc cggacgtgac cggctcctat
     1861 gccagattcg ctgcctcctg catcgtccgc atcctcaact ggtcacgatg ccggaggaag
     1921 gtatccgccg tggtgttcca aacgccgatg cgtcgagaca ggtctttcca ggcaagctcc
     1981 cacggtgttc ggccttccat gcgtgggcaa cgctacccta gccaccgcac ggcaccgacc
     2041 gaccttgctg ccgcctcaca tcgcgttcag atggctgctc cggcgatcgg gctccgcgag
     2101 tgggacgcct gaattaccgc ccgccgcgct tcgtgggcgc gctcgatccc tcgatctggt
     2161 cgagcgcccg ggtcagcagc gagcgcaccg catcggcgcg ggtgaagtcc agcccggggt
     2221 gctcggtcgt cattcggtcg acgtgccgat cgagccgggc gatcaggctg tcgggcaagc
     2281 ggaatgcaac ctgcggggtg ggtt
//
LOCUS       DASVOR010000495        10217 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_442787,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000495 DASVOR010000000
VERSION     DASVOR010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10217
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442787"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VF516_09785"
     CDS             <1..628
                     /locus_tag="VF516_09785"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX8108007.1"
                     /translation="PRLYDRSSPVASSKLRRLIELVGELRAEGHRALVFSQFTSHLAL
                     VRDALAELAIPALYLDGETPAAQRKRRVQAFQAGEADVFLISLTAGGTGLNLTAADYV
                     FHLDPWWNPAVEDQATDRAHRIGQDRPVTVYRLIARGTIEDTILALHADKRALIAGVL
                     DGTSAAARLTTRDLLALISSGPAPRAAEPQDDDEPAPPRPVPHLRLVP"
     gene            complement(669..1046)
                     /locus_tag="VF516_09790"
     CDS             complement(669..1046)
                     /locus_tag="VF516_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108008.1"
                     /translation="MKVILDAGAFVAIERRDRRIEAMLRVLHRQRVPLWTSAAVVAQV
                     WRDDRKQALLARILPGVGVRALAPGDDTRIGELLAVTRTSDVIDGHVALLADDTDRIV
                     TSDPDDLARLIEARGVDAEVVTV"
     gene            complement(1043..1294)
                     /locus_tag="VF516_09795"
     CDS             complement(1043..1294)
                     /locus_tag="VF516_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108009.1"
                     /translation="MNVDRLSITLDPRLGAAARKAAKRARVSLSTWIAEATADRIRNE
                     LLGEALDRWEAEDGALTRAELDRAAEALGLSRRRKARRA"
     gene            complement(1387..2037)
                     /locus_tag="VF516_09800"
     CDS             complement(1387..2037)
                     /locus_tag="VF516_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108010.1"
                     /translation="MTAARLVPALLLVVAACLPLTWNEISPGAPPRDGTIAVVGELTL
                     IPPVEQQTRAYSGVVLVGAARDRMYGVFTTDLKRPFGPDLWEDGDAHYSVLLPLEGPF
                     FIEIPRRPWRLYLRGVVVMTDRGRTGVEVPVQIAIRPEDDIVYIGHLYVQRTSPRWTQ
                     VREEESAARQAARQIGHVLATRPWTVQLARPLENGGYPSSSDKVFAPVPLQVPPAH"
     gene            complement(2034..2531)
                     /locus_tag="VF516_09805"
     CDS             complement(2034..2531)
                     /locus_tag="VF516_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108011.1"
                     /translation="MKPLRAPEQVGDEFSLVFLSIQREGGLFASDIDTVTVRRVDSPA
                     RGYIFWTTSEFLFRVFDTRTVKDGHFLIQLGPGVYELDRLDSGGTHWLLADEARVASR
                     IYVTRPGVYDLGTLRLDRSGLFGKYSLAAQGDAGSAERSQQLWDAVKGTSWERLVASP
                     ARDTR"
     gene            complement(2603..3520)
                     /locus_tag="VF516_09810"
     CDS             complement(2603..3520)
                     /locus_tag="VF516_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108012.1"
                     /translation="MTLLCGLCGLVGSARADPNGLAGGRLLLRAGTSEVSLAAARAAV
                     GGDKIVLELRRGAQVLEASLDEDGYFWLKAPAGAYRLEYLRVGKRAEFFAPQEIVVQP
                     AALTCAGTIALELDQIESLGANVNNRVTVTDQCAETTPRLRRVAGTAAERGIERVVIA
                     QPAPQFEHFSHLGWRDLVIGLRAEAAVSDHPIVRGLVRLPPLAREGVFSRWILLVGVG
                     GLRGDRNALVYDVTLGAGVHVAVFDLLAVTGARWPEAQGVQVGPVVGGVARLHSTIMG
                     LGVRVEALPSRAAFFTIDLAPLGLLGSLL"
     gene            3985..5679
                     /locus_tag="VF516_09815"
     CDS             3985..5679
                     /locus_tag="VF516_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108013.1"
                     /translation="MRTARVLLVLAILGCGHHSTGPGTGTPDGNCDPSDPACGGMTGS
                     GDVRMFPKASLIIPMDLSYQSTGMFQAYGLVYQLLRGGIHVYWVIDPQKTYHVAPCNT
                     PGDLCAWDCGVQGSGVKCSYPTSSPDFVATTSVIWDDTGKAARGSALGAHGYRGGPFV
                     VDAADHDAALKIIDAWNDQAHATRSVFHVVSVHETTAAFTGNIGRAMAAPPTIAVFAD
                     GNENIATGYLRAAGIPQSNGAEFPATTCGASNCGPSTANPDLLTEEAIAGDLGTCAAP
                     NHDHHNGALFKPDGTPAYCQIMSMHWGVTEREKVQCDGGNCPATQAQCTGQKFTFNGH
                     EVVAEVRAFLANNVHFFAECQAVDAYENTTPNPAWPFLDDMGRDGHFLTTTGTPPLCP
                     AGSCADSNYQCVQNACAGQACCLPKPQTWQNMPGYEVAAQPTTGTVKVLRPEIPYNQL
                     DGEFGTTGGSEPAYNLSAYLGTTYKNDRQVTLLTGPSGPGDQDLWMTGFLDGCGQVIL
                     RTTGEAPAAATECGGKISYLGGHQYQTTVPVTSGSQSQGTRLFLNALFEAQCVTGP"
     gene            complement(5760..6845)
                     /locus_tag="VF516_09820"
     CDS             complement(5760..6845)
                     /locus_tag="VF516_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108014.1"
                     /translation="MVTANDLMASFAERSGLIDARPQRRYLWTDAFAVCNFLELGQLD
                     LARALVGSVHHELGRHRPGGARRGWLSGLGDADGEAHPTLGGLRIGKPLPERGDGEPL
                     DERLEWDRDGQYFHYSTKWMHALDQVTRATGNERYTIWARELADTAHRRFVHGPHGRR
                     RMHWKMSVDLMRPQVASMGHHDPLDGYVTCLQLDATTRRGPDLGEAIADYRSMVDPDR
                     LATGDPLGIGGLLVDAHRLTQLDRDRPLRDALLDAARAGLAHYVKQPDLRLPADHRLA
                     FRELGLAIGLAAARAMNAPGVAPHDWLRGAIVAFWLDGEHRQSPTYRDHQDINDVMLA
                     TSLQPDGFLVLRRDAGLRAGPRVLLAE"
     gene            complement(6948..8099)
                     /locus_tag="VF516_09825"
     CDS             complement(6948..8099)
                     /locus_tag="VF516_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108015.1"
                     /translation="MIFSVSIDARLFVLAGQSFPFEVCGLLDRRSSQAASARPIRATD
                     TMGDSKPRASATAMSSGQNACGLRSAVSCAQQDREPKDRGLRRRTSWCRSLIARARSG
                     AIDRKRRACTMRWRRAMLAWSGPQRRSESPGTRPARRASMTMRRAWLTVLAFACAQPA
                     ALGDPPDAPPSARFEHDMIVRFHMHENFALVRAIEKLLIRGRLEEARAFAQAIANAPD
                     EPGLGAWSRHAALVRNRAAALATARGGDEACRRAVQLADACAGCHVESGASPEFRSPG
                     QAPPDGPTIEARMARHLWATDRLWEGVVGGADDAWLAGLDVLSSAPPQASELSREQAA
                     FGRKLQRLADQARSRQRTGTAQDRVRSYGEILVTCSGCHAAAPAASRPR"
     gene            8639..9097
                     /locus_tag="VF516_09830"
     CDS             8639..9097
                     /locus_tag="VF516_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8108016.1"
                     /translation="MTFKKILCPVDFSEDSQQAMQVAAGLARNAQATLVLLYVWQPQR
                     WMTDYGMQWPNEVLVEAQALEDGKLATYRVDAQRLGAPEVVTRLVRGEPWDQIVSAAH
                     DDRAIDLIVMGTHGRTGLRRALIGSVAERVVRHAPCTVMVVRAPQPERPA"
     gene            complement(9150..9776)
                     /locus_tag="VF516_09835"
     CDS             complement(9150..9776)
                     /locus_tag="VF516_09835"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8108017.1"
                     /translation="MMARSILWIGAVVVVLSAVTRAAPPAAEQVIRRGVDDVLDVLRD
                     PATQPAAARAQRIARLRQVADRLFDWPEMSQRSLGPTWRTIDAPQRKRFAEVFPDLLA
                     SAYLDDLDKFRGDERVTIERSTESGDHAEVYTIVVTHGGERVPMVYWLHRVESAWRVY
                     DFSIEGVSLVNHYRESFTRFLVNHRFEELMERLERKRPQATNSVSRDR"
     gene            complement(9773..>10217)
                     /locus_tag="VF516_09840"
     CDS             complement(9773..>10217)
                     /locus_tag="VF516_09840"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8108018.1"
                     /translation="VYDPARHFVPGIGLGLRWELWGDLAGGRADEQRARGAELRHLER
                     WARTALSADVTNAYEELERARADLPALAEATALAKEWVVRANADYAVGLVDSISLTGA
                     VDAYLTNRLAELEAVYRFNVALARLSRAIGAGAGGESLYAEGGTP"
BASE COUNT         1599 a         3700 c         3518 g         1400 t
ORIGIN      
        1 tccccggctg tacgaccgct cgtcgccggt cgcgtcgtcg aagctgcgcc ggctgatcga
       61 gctggtcggc gagctgcgcg ccgagggcca ccgcgcgctg gtgttcagcc agttcacgtc
      121 gcacctcgcg ctggtccgcg acgcgctggc cgagctcgcg atcccggcgc tgtacctcga
      181 cggcgagacg ccggccgcgc agcgcaagcg ccgggtccag gcattccagg ccggtgaggc
      241 cgacgtgttc ctgatctcgc tgaccgccgg cggcaccggc ctcaacctga ccgccgccga
      301 ctacgtgttc catctcgatc cgtggtggaa tccggcggtc gaggatcagg ccaccgatcg
      361 ggcgcaccgg atcggccagg accgcccggt cacggtctac cggctgatcg cccgcgggac
      421 gatcgaggac acgatcctcg cgctgcacgc cgacaagcgc gcgctgatcg ccggcgtgct
      481 tgacggcacc agcgcggccg cgcggctcac cacccgcgac ctgctcgcgc tgatctccag
      541 cggtccggcc ccgcgcgcgg ccgagccgca ggacgacgac gagcccgcgc cgccgcgccc
      601 cgtaccacac ttgcggctcg tgccctgacc gacgatgcca cgtgtagcgg tcgggaggcc
      661 gagcgggctc agaccgtgac gacctcggca tccacgccgc gggcctcgat caatcgtgcg
      721 agatcgtcgg gatcacttgt gacgatgcga tccgtgtcgt cggcaagcag cgcgacgtga
      781 ccgtcgatca cgtcgctggt tcgggtgacc gcaagcagct ctccgatgcg ggtgtcgtcg
      841 cccggcgcaa gcgctcggac cccgaccccg ggcaggatcc gcgcgagcag ggcctgcttg
      901 cgatcgtccc gccagacctg ggcgacgacc gcggcgctgg tccacagcgg cacgcgctgc
      961 cggtggagga cgcgcagcat tgcttcgatc cgccgatctc gccgctcgat ggcgacgaac
     1021 gcaccggcgt cgaggatcac cttcacgcgc gtcgcgcctt gcggcgccgc gacaggccga
     1081 gggcctcggc ggctcggtcc agctctgctc gggtcaacgc gccgtcttcg gcttcccacc
     1141 gatcgagcgc ctcacccagc agctcattcc ggatccggtc ggcggtcgcc tccgcgatcc
     1201 aggtcgagag gctcaccctg gcgcgcttgg ccgccttgcg tgccgcggcc ccgagcctcg
     1261 ggtccagggt gatcgagagg cggtcgacgt tcatgcatac gaccgtatta cacaccacta
     1321 tatgcgtcaa gccacggctc gcagcgtacg gcgtcgccct cgacgatcag ctcgctcccg
     1381 acgtcatcaa tgagcgggcg gaacctggag cggaaccggc gcaaacacct tgtcgctcga
     1441 cgacgggtag ccgccgttct cgagcgggcg cgccagctgg acggtccacg gccgggtcgc
     1501 cagcacatgg ccgatctgac gggcggcctg gcgcgccgcc gactcctcct ccctgacctg
     1561 ggtccagcgc ggcgaggttc gctgcacgta gagatggccg atgtacacga tgtcgtcctc
     1621 gggtcggatc gcgatctgga ccggcacctc gacgccggtc ctgccgcgat cggtcatcac
     1681 gacgacgcct cgcaggtaca gccgccaggg gcgccgtggg atctcgatga agaacggccc
     1741 ctcgagcggc agcaagaccg agtagtgcgc gtctccgtcc tcccagagat cgggaccgaa
     1801 cgggcgcttc agatcggtgg tgaagacacc gtacatccgg tcgcgcgccg cccccaccag
     1861 caccactccc gagtacgccc gggtctgctg ctcgaccggc gggatgaggg tcaactcgcc
     1921 gacgacggcg atcgtgccgt cccggggcgg cgcgcctggc gagatctcgt tccaggtgag
     1981 tggcaagcac gccgccacaa ccagcagcag cgccgggacc aggcgcgcgg ccgtcaccgg
     2041 gtgtcccgcg ccggggaagc cacgaggcgc tcccaggacg tgcccttcac cgcgtcccac
     2101 agctgctggg agcgttcggc gcttcccgcg tccccctgcg cggcgaggct gtacttgccg
     2161 aacaaccccg atctgtcgag ccgcagcgtc ccgagatcgt agacgccggg gcgcgtgacg
     2221 tagatccggc tcgccacgcg cgcctcgtcc gccagcagcc agtgggtccc cccgctgtcc
     2281 aggcggtcga gctcgtagac gcccggaccc agctggatca agaagtggcc gtccttgacc
     2341 gtccgggtat cgaacacccg gaacaggaat tcactcgtgg tccagaagat gtatccgcgc
     2401 gccggcgaat cgacgcgccg gacggtcacg gtgtcgatat cgctcgcaaa caggccgcct
     2461 tcgcgctgga tgctcaggaa caccagcgag aactcgtcgc ccacctgctc cggggcacgc
     2521 agcggcttca tcgcgtagat cggatcgctc agccgaaaac acgcgccgtg caagacgagc
     2581 cagaggacga gcagccgtcg attcatagca agctccccag cagcccgagc ggtgcgagat
     2641 cgatggtgaa gaacgccgcg cgcgagggca gcgcctcgac ccgcacgccc agccccatga
     2701 tcgtcgagtg caggcgggcc acgccgccca cgaccggccc gacctggacg ccttgcgcct
     2761 cgggccagcg cgcgccagtg accgccagca ggtcgaagac ggccacgtgg actcccgcgc
     2821 cgagtgtgac gtcatagacc agcgcattgc gatcgccgcg aagcccacct acacccacca
     2881 gaaggatcca gcgcgagaac accccctccc gcgccagcgg tggcaggcga accagcccgc
     2941 gcacgatcgg gtgatcgctg acggcggcct cggcgcgcaa tccgatcacg agatcgcgcc
     3001 accccaggtg gctgaaatgt tcgaactgcg gtgccggctg cgcgatgacc acgcgctcga
     3061 tcccgcgctc ggcggccgtt cccgcgaccc tgcggagccg cggcgtcgtc tcggcgcact
     3121 ggtcggtcac ggtgacgcga ttgttcacgt tggcgccgag ggattcgatc tgatcgagct
     3181 cgagcgcgat ggtgccggca caggtcagcg ccgcgggctg gacgacgatc tcctgcggcg
     3241 cgaagaactc ggcgcgcttg ccgacgcgca gatactcgag ccggtaggcg ccggcaggcg
     3301 ccttgagcca gaaatagccg tcctcgtcca ggctcgcctc gagcacctgc gcgccgcgcc
     3361 gcagctcgag cacgatcttg tcaccgccca ccgccgcgcg tgccgcagcg agcgagacct
     3421 cgctggtccc cgcgcgcagc agcaagcggc ctccggcgag gccattcgga tcggcccggg
     3481 cggagccgac cagaccgcac aggccgcaca gcagcgtcac cccgagaaca cgcatggatg
     3541 tcatgcccgg cgctacagta cgtgattcct gcccccgccg ctcgcgccac gcactgccgg
     3601 gccgccggcg gtgcacgagc tcgcttctcg cgaatctgag ccgttgcgcc ttgtcatcaa
     3661 aagatagcga cccatccgca cgaccacgac ctggcatctg cgcaagaacc gctcagcgcg
     3721 acacttgcga cagtatggca atgtgggtgg acccctccgg actccggtca tttcgtccgg
     3781 agtccggaca gtggttcagc gcgtcgcctc gcgagccgac ctccgattgt gagcctcgca
     3841 cgccccgtgt cgatccccga tcggcgacgc gcgggagcgt gtagtatccc cgcacgcgca
     3901 cccgcgcggc gccactccac catgtggact ccatcggacc gtgatgcgtc gtgcgcacgg
     3961 ccggcgatgc atcaggaggc tgtgatgcgc actgctcgcg tcttgctcgt tctcgccatc
     4021 ctcggttgcg gacaccactc gaccggaccc ggaaccggaa cccccgacgg caactgcgat
     4081 ccctcggatc cggcgtgcgg cgggatgacc ggcagcggcg acgtccgcat gttcccgaag
     4141 gccagcctga tcatcccgat ggacctcagc taccagagca ccggaatgtt ccaggcctac
     4201 gggttggtct accagttgct ccgcggcggc atccacgtgt actgggtgat cgacccgcag
     4261 aagacctacc acgtggcgcc gtgcaacacg cccggcgatc tctgcgcgtg ggactgcggc
     4321 gtgcagggct cgggcgtcaa gtgcagctat ccgacctcga gcccggactt cgtcgcgacg
     4381 accagcgtga tctgggatga caccggcaag gccgcgcgtg gcagcgcgct cggcgcgcac
     4441 ggctaccgcg gcgggccgtt cgtggtcgac gcggccgacc acgacgccgc gctgaagatc
     4501 atcgacgcct ggaacgacca ggcccacgcg acgcgcagcg tgttccacgt cgtgtcggtg
     4561 cacgagacca cggcggcgtt caccggcaac atcggccgcg cgatggccgc gccgccgacg
     4621 atcgcggtgt tcgccgacgg caacgagaac atcgcgaccg ggtacctgcg tgccgcgggc
     4681 atcccgcaat ccaatggtgc ggagttcccg gcgacgacgt gcggcgccag caactgcggc
     4741 cccagcaccg ccaaccccga tctgttgacc gaggaggcga tcgccggcga tctcgggacg
     4801 tgcgcggcgc ccaaccacga tcatcacaac ggcgcgctgt tcaagcccga cggcacgccc
     4861 gcgtactgcc agatcatgtc gatgcactgg ggcgtcaccg agcgcgagaa ggtccagtgc
     4921 gacggcggga actgcccggc gacccaggcc cagtgcaccg gccagaagtt cacgttcaac
     4981 ggccacgagg tcgtcgccga ggtccgggcg ttcctggcca acaacgtgca cttcttcgcc
     5041 gagtgccagg cggtcgacgc gtacgagaac accacgccga acccggcgtg gcctttcctc
     5101 gacgacatgg gccgggacgg tcacttcctg accacgaccg gcacgccgcc gctgtgcccg
     5161 gccggcagct gcgcggacag caactaccag tgcgtgcaga acgcgtgcgc cggccaggcg
     5221 tgctgcctgc cgaagccgca gacctggcag aacatgcccg gctacgaggt cgccgcccag
     5281 cccaccaccg gcacggtgaa ggtgctgcgg ccggagattc cctacaacca gctcgacggc
     5341 gagttcggca cgaccggcgg cagcgagccg gcctacaacc tgagcgcgta cctcgggacg
     5401 acgtacaaga acgaccgcca ggtcacgctg ctcaccggcc ccagcggacc gggggatcag
     5461 gatctgtgga tgaccggctt cctcgacggc tgtggccagg tgatcctgag aaccaccggg
     5521 gaggcccctg cggcggcgac cgagtgcggc ggcaagatca gctacctcgg cgggcaccag
     5581 taccagacca cggtcccggt gacctcgggc tcgcagagcc agggcacgcg gctgttcctg
     5641 aacgcgctgt tcgaggcgca gtgcgtgacc ggcccgtgag gcggctgccg gctcacggcg
     5701 tgacggcggc ggccgtgccg atcgcgcggc gccgccggcc gagccagccc ttggcccggt
     5761 cactcagcaa gtagaacgcg gggcccggcg cggagacccg cgtcgcggcg gagcaccagg
     5821 aacccgtcgg gctggaggct ggtcgcgagc atcacgtcgt tgatgtcctg gtggtcgcgg
     5881 taggtcggtg actggcgatg ctcgccgtcg agccagaacg cgacgatcgc gccacgcagc
     5941 cagtcatgcg gcgccacgcc gggggcgttc atcgcgcgcg cggcggcgag accgatcgcg
     6001 aggccgagct cgcggaacgc gagccgatgg tccgcgggga gccgcaggtc gggctgcttc
     6061 acgtagtgcg cgagaccggc gcgcgcagcg tcgagcagcg cgtcgcgcag cgggcgatcg
     6121 cggtcgagct gggtgagtcg gtgggcgtcg acaagcagcc cgccgatgcc gagcggatcg
     6181 ccggtcgcga gccggtccgg atcgaccatg ctccggtagt cggcgatcgc ctcgccgaga
     6241 tccggaccgc gccgggtcgt cgcgtcgagc tgcaagcacg tgacgtagcc gtcgagcggg
     6301 tcatggtgac ccatcgacgc gacctgcggc cgcatcaggt cgacgctcat cttccagtgc
     6361 atccggcggc ggccgtgcgg tccgtggacg aatcgccggt gcgcggtgtc ggccagctcg
     6421 cgcgcccaga tcgtgtaccg ctcgttcccg gtcgcgcgcg tcacctgatc gagcgcgtgc
     6481 atccacttgg tcgagtaatg gaaatactgc ccgtcgcggt cccattcgag ccgttcgtcg
     6541 agcggctcgc cgtcaccgcg ctcgggcagc ggcttgccga tgcgaagacc gccgagcgtg
     6601 gggtgcgcct cgccgtcggc atcgccgagc ccgctgagcc atccgcggcg tgcacctccc
     6661 gggcgatggc ggcccagctc gtggtgcacg ctaccgacga gcgcgcgcgc aagatcgagc
     6721 tgaccgagct cgaggaagtt gcagaccgcg aacgcgtcgg tccacagata gcggcgctgc
     6781 ggccgcgcat cgatcagacc gctgcgctcc gcgaagctcg ccatcaggtc gttcgccgtg
     6841 accatgcaga cactgtggat acggcgtgcg tggatgcaag cccgccaggt cgaccgagca
     6901 gggcgcagcc aggccgacct gctcggctgg ccaggacccg cggtggatca gcggggccgg
     6961 ctcgctgcgg gcgcggctgc gtgacatccg gaacacgtca ccaggatctc gccatagctg
     7021 cggacgcggt cctgcgcggt ccctgtgcgt tgccgcgagc gagcctgatc ggcgagccgc
     7081 tgcagcttgc gcccgaatgc ggcttgctcc ctggagagct ccgacgcttg cggtggagcc
     7141 gaggacagca cgtcgagtcc ggcgagccac gcatcgtcgg cacctccgac gacgccctcc
     7201 cacagccggt ccgtggccca cagatgccgt gccatccgcg cctcgatcgt cggcccgtcc
     7261 ggtggagcct gtccaggcga ccggaactcc gggctcgctc ccgattcgac atggcagccg
     7321 gcgcaggcgt ccgcgagctg gacggcacga cgacacgcct cgtcgccacc gcgggcagtt
     7381 gccagcgccg cggcgcggtt ccggaccagc gccgcgtgcc gcgaccaggc gcccagcccc
     7441 ggctcatcgg gcgcgtttgc gatcgcctgc gcgaaggcgc gtgcttcctc gagccgcccg
     7501 cggatgagca gcttctcgat ggcgcgcacg agcgcgaagt tctcgtgcat gtgaaatcgc
     7561 acgatcatgt cgtgctcgaa gcgagcagag ggcggcgcat cgggcgggtc gcccagggcg
     7621 gcgggttgcg cgcaggcaaa tgcaagaacg gtcagccatg cccggcgcat cgtcatgctg
     7681 gcgcgtcgtg caggacgtgt tccaggggat tccgacctgc gctggggtcc ggaccacgca
     7741 agcattgcgc gccgccatcg catggtgcac gccctgcgtt tcctgtcgat cgcgccgctg
     7801 cgcgcccgcg cgatcaacga gcggcaccac gaggtccgcc gacgcagacc gcgatctttc
     7861 ggttcgcgat cctgctgcgc acacgagacc gctgaacgca atccgcacgc gttctgaccg
     7921 gaggacatgg cagttgcgga ggcacgcggc ttgctgtctc ccatggtatc ggtggctcgg
     7981 atgggccgcg cggacgcggc ctgactggag cgcctgtcca gcaggccaca aacctcgaac
     8041 gggaaggact gcccagcaag cacgaagagc cgcgcatcga tcgatacgct gaagatcacc
     8101 gccggggccc acgtgacggc gcaagatcaa gagcggacgg ggcgttagca ccgcctcgaa
     8161 ggtgcgcggt cgaaagcccg aaggagaaca gccgacgaga cgaacgcgca ccggcagatc
     8221 gtcctgcccc tgtggcgaac gcgactccga tgccccgagg ccggtcctgc aacggcacgg
     8281 agggtggaac tggagacgac ccacaacggt cggagcgcgg ggaactctcg tcgagccagc
     8341 gcaggcgtcc gcgcggtcca tccgagccgc cgaccgtgcc acgagcgatg ccgccgacct
     8401 ccggcgccgc ggcgcgctgg atccgcgtgg ctgtgccacc ggcaagcgcc gtgctgctac
     8461 gggcccgccc gcggtctccc ggcgcgatgc tcggcggccg gcgcatgctc ggcgccgcag
     8521 gaactgcgtg gcgacggcct cggcgtgaac ctcttgcgcg gtcgcggtgc cccaccggca
     8581 ccagctcggg cacatcccac gccccggcgc aggcacgcca cgtgctgcaa ggccggtcat
     8641 gaccttcaag aagatcctgt gccccgtcga tttctccgaa gactcgcagc aagcgatgca
     8701 ggtcgcggcc ggactggccc gcaacgcgca ggcaacgctc gtgctgctct acgtctggca
     8761 accgcagcga tggatgaccg actacggcat gcagtggccc aacgaggtgc tggtggaggc
     8821 ccaggcgctc gaggacggca agctcgcgac gtatcgcgtg gacgcccagc ggctcggcgc
     8881 gccagaggtc gtgaccaggc tggtgcgcgg tgagccgtgg gatcagatcg tgtcggctgc
     8941 ccacgacgac cgcgcgatcg acctgatcgt gatgggcacg cacgggcgca ccggcctccg
     9001 gcgcgcgctg atcggctcgg tcgccgagcg ggtcgtgcgt cacgcgccct gcacggtgat
     9061 ggtcgtccgg gcgccgcagc ccgagcggcc tgcgtaggcc cggcccgcgc ccgtccagcc
     9121 cagctgctcg ggcggccggc gcgccgcgat caccggtcgc gagacaccga gttcgtggct
     9181 tgcggtcgct tgcgctccag tcgctccatg agctcttcga agcggtgatt gacgaggaag
     9241 cgggtgaacg actcgcggta gtggttgacg aggctgacgc cctcgatgct gaagtcatag
     9301 acccgccacg ccgattcgac gcggtgcagc cagtagacca tcggcacgcg ctcgccgccg
     9361 tgggtgacga cgatcgtgta gacctcggcg tggtcgccgg actcggtcga gcgctcgatc
     9421 gtgacgcgtt cgtcgccgcg aaacttgtcg aggtcgtcga ggtaggcgct ggcgaggagg
     9481 tcggggaaca cctcggcgaa ccgcttgcgc tggggggcgt cgatcgtgcg ccaggtcggc
     9541 cccaggctgc gctgcgacat ctccggccag tcgaacaacc ggtcggccac ctgccggaga
     9601 cgcgcgatgc gctgcgcccg cgccgccgcc ggctgggtgg ctggatcgcg caggacgtcc
     9661 aggacatcgt cgacgccgcg gcggatcacc tgctcggccg ctggcggcgc cgccctggtg
     9721 accgcggaga gcacgacgac caccgcgccg atccacagga tcgatctcgc catcatggcg
     9781 taccgccttc cgcgtacagc gactcgccgc cggcgccggc gccgatcgcg cgcgacaggc
     9841 gcgccagcgc gacgttgaac cggtacaccg cctcgagctc cgcgagccgg ttcgtcaggt
     9901 aggcgtcgac ggcgccggtc agcgagatcg agtccaccag cccgaccgca tagtcggcgt
     9961 tggcgcgtac cacccactcc ttggccagcg cggtcgcctc cgccagcgcc ggcagatcgg
    10021 cccgagcacg ctcgagctcc tcgtaggcat tggtgacgtc ggccgacaat gcggtccgcg
    10081 cccagcgctc gaggtgccgc agctcggcgc cccgtgcgcg ctgctcatcg gcgcgcccac
    10141 ccgcgaggtc gccccacagc tcccagcgca gcccgaggcc gatcccggga acgaagtgac
    10201 gggccggatc gtagacg
//
LOCUS       DASVOR010000496         4021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238133,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000496 DASVOR010000000
VERSION     DASVOR010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238133"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VF516_09845"
     CDS             <1..639
                     /locus_tag="VF516_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108019.1"
                     /translation="PAVWMSLVYTAVDFDGDGFTAPAMGTRCTAGTLLPPDFATPRGN
                     DCNDSDPSVWVAQTVFADTDHDGFGAGPALAACTNGAPPEGFVTSGTDCDDTDASVWA
                     LLAYSAIDADGDRVTVPASGQVCTDGTLPAPFKATPHGNDCDDHDPTIDHFAVLYLDL
                     DGDGVGAPPRQVRCIGATVPAGLSRGGFDDDDTDPDVIEADDEELDLLLLGD"
     gene            complement(646..804)
                     /locus_tag="VF516_09850"
     CDS             complement(646..804)
                     /locus_tag="VF516_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108020.1"
                     /translation="MRSDWRDDLTREGLAPVAVSVAEDAEHLPCPACGTAAPLVDGAC
                     SDCGLQLE"
     gene            complement(862..1026)
                     /locus_tag="VF516_09855"
     CDS             complement(862..1026)
                     /locus_tag="VF516_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108021.1"
                     /translation="MPRITAAEAEELLDGLPLIPAMAGSIPEMKALRDRCLAAGIPVL
                     VGCPPGAGKG"
     gene            1163..1528
                     /locus_tag="VF516_09860"
     CDS             1163..1528
                     /locus_tag="VF516_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108022.1"
                     /translation="MKNAKLALVGCGVLGLVALIVPFAGRSLLVDMLEIDPSSALVYA
                     AIFVLPAAMGTLGLSRPPLRAWQSGVSLAACVLGVVRLQVWDLALHLPSIGLRGVLLL
                     AAIVIGTVAAVTTLVRPEA"
     gene            complement(1555..3420)
                     /locus_tag="VF516_09865"
     CDS             complement(1555..3420)
                     /locus_tag="VF516_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8108023.1"
                     /translation="MSHPPPSSPPGSPPRKHSPSAAPLAEDFVDFEGDPTSINQIPSA
                     QTHGTQPASRPSIPTISLPAPGPPGQFASASPSLSHIGSPSGQQYGTNPGLSVHGMVP
                     QAQHAPHQGHQGAPGAMNGGMTMTAMPGPPVAQVSGVIAQPTPYVSRSRIYAFVVDET
                     GMPIELGSGRFAKCYLGEERWVESKTTLRRPVAIKALQRGVSAEDQMRFQMEKEILER
                     VQGHPNIVELLGSGEGDSAGFVPPMIRDRVENDFMILELLDMSLEERLKGARQKRRRD
                     DLLAVPLRERIFRVLEYVVPVATAVEFSHLVRDTCHRDIKPANILVKLPDPNLRGSQM
                     KVKLADFNVGKVADPDLDVSMTRFQAVPGTLYFQSPEQETNTFELLVNCTQGSPEVEF
                     FEDFYIDIYENDTFSLFNRNETYSIAAADRARKKLLLNRPFAEPSETNVRGRVVKSVG
                     RPADIYSLGAVFYYLISGAYANPKNLFDAFRKFVEYEKRDENNSIAAYIEHEYRTIQN
                     IRAPKAEGQGAPDLAPEDRFFSYKHYLDGNGELIDPLIMMIIAKAMIRNTPDSYCLSY
                     DLRTTGISAMVHDLMSLYIAYGIDPSARIAYANDGYQAPKKTGAVRRAFDRFFKR"
     gene            complement(3429..>4021)
                     /locus_tag="VF516_09870"
     CDS             complement(3429..>4021)
                     /locus_tag="VF516_09870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108024.1"
                     /translation="PQQTAPRPESTPPPQPLSSPGWAPPPAVTPWMLRVPVPSIGPQG
                     IPHRLPLSDVPQVVGDRRVHYLHVPNQVADLIGSHNSKVRVTFLSGPTGGRVLHLKGL
                     TCFVAPLGGRPSPAVQIDQASDVALVTPRAQEIGHIRVATGTPGAGHTVFPVGGELVS
                     VALDDCIDAILFDFGPGSEAYFVYTRGRPIPKSRRG"
BASE COUNT          566 a         1294 c         1544 g          617 t
ORIGIN      
        1 ccggcggtgt ggatgtcgct ggtgtacacg gcggtcgact tcgatggcga tggcttcacg
       61 gcgccggcga tggggacgcg gtgcacggcg gggacgctgc tgccgccgga cttcgcgacg
      121 ccgcgcggca acgactgcaa cgacagcgac ccgagcgtgt gggtcgcaca gacggtgttc
      181 gccgacaccg accacgacgg cttcggggcc gggccggcgc tggcggcgtg cacgaacggc
      241 gccccgccgg agggcttcgt caccagcggc accgactgcg acgacaccga cgcgtcggtg
      301 tgggcgctgc tggcgtacag cgcgatcgac gccgacgggg acagggtcac ggtgccggcg
      361 agcggccagg tctgcaccga cgggacgctg ccggcgccgt tcaaggcgac gccgcacggc
      421 aacgactgcg acgatcacga cccgacgatc gaccacttcg cggtgctgta cctcgatctc
      481 gacggcgatg gcgtcggcgc gccgccgcgc caggtccgct gcatcggcgc cacggtgccg
      541 gcggggctgt cgcgcggcgg gttcgacgac gacgacaccg atcccgacgt gatcgaggcc
      601 gacgacgagg agctcgatct gctgctgctg ggcgactgag gcggctcact cgagctgcag
      661 gccgcagtcg gagcaggcgc cgtcgaccag cggcgcggcg gtgccgcacg ccgggcacgg
      721 caggtgctcg gcgtcctcgg cgacgctgac cgcgacgggc gccaggccct cgcgggtgag
      781 gtcgtcgcgc cagtcggagc gcatcagctc ggcgagccgc ggcagctcgt cctcctcgac
      841 caggaggtgg gtccgcgtgc ctcaaccctt gccggcgccg ggcgggcagc cgaccaggac
      901 cgggatgcct gcggccaggc agcggtcgcg cagcgccttc atctcgggga tcgagccggc
      961 catcgcgggg atcaggggga gtccgtcgag cagctcctcg gcctcggcag cggtgatgcg
     1021 cggcatcggc caaggctacg gcatgtccgc cgcggttcca accggcgcgg catcgcggcc
     1081 ggcaagtgtc atgatgaagc atgcccggcc ggcgtcgaat ctccagatcg gaaccggcga
     1141 gccggcgttg caaggagtac ccgtgaagaa cgcaaagctc gcgctcgtgg ggtgtggggt
     1201 gctcggcctg gtcgccctga tcgtcccgtt cgctggtcgg tcgctgctcg tggacatgct
     1261 ggagatcgac ccgtcgtccg cgctggtcta cgcggcgatc ttcgtgctgc cggcggcgat
     1321 gggcacgctc gggctgtccc ggccgccgct gcgggcctgg cagtcggggg tctcgctggc
     1381 ggcctgcgtg ctcggcgtgg tccggctcca ggtctgggac ctcgcgctgc acctgccgag
     1441 catcgggctg cgcggggtgc tgctgctcgc ggcgatcgtg atcgggacgg tggccgcggt
     1501 gacgacgctg gtgcggccgg aggcctgagg ccgtgcgacg ctgccgaggc gccgctaccg
     1561 cttgaagaac cggtcgaacg cgcggcgcac cgcgccggtc ttcttgggcg cctggtagcc
     1621 gtcgttggcg tacgcgatcc gcgccgacgg gtcgatgccg taggcgatgt acagcgacat
     1681 gaggtcgtgc accatcgccg agattccggt ggtccgcagg tcgtacgaca ggcagtagct
     1741 gtcgggcgtg ttgcggatca tcgccttggc gatgatcatc atgatcagcg gatcgatcag
     1801 ctcgccgttg ccgtcgaggt agtgcttgta gctgaagaac cggtcctcgg gggcgaggtc
     1861 cggggcgccc tggccctcgg ccttgggcgc gcggatgttc tggatcgtgc ggtactcgtg
     1921 ctcgatgtac gcggcgatcg agttgttctc gtcgcgcttc tcgtactcga cgaacttgcg
     1981 gaacgcgtcg aacaggttct tggggttcgc gtaggcgccc gagatcaggt agtagaacac
     2041 cgcgccgagc gagtagatgt cggcggggcg gccgacgctc ttgaccacgc ggccgcgcac
     2101 gttggtctcg ctcggctcgg cgaacggccg gttgagcagg agcttcttgc gcgcgcggtc
     2161 ggcggcggcg atcgagtagg tctcgttgcg gttgaacagc gagaacgtgt cgttctcgta
     2221 gatgtcgatg tagaagtcct cgaagaactc gacctcgggc gagccctggg tgcagttgac
     2281 cagcagctcg aacgtgttgg tctcctgctc ggggctctgg aagtacagcg tgcccgggac
     2341 cgcctggaac cgggtcatcg acacgtcgag gtcggggtcg gcgaccttgc cgacgttgaa
     2401 gtcggcgagc ttgaccttca tctgcgagcc gcgcaggttg ggatccggca gcttgaccag
     2461 gatgttggcg ggcttgatgt cgcggtggca ggtgtcgcgg accaggtggg agaactcgac
     2521 cgcggtcgcg accgggacga cgtactcgag cacgcggaag atccgctcgc gcagcggcac
     2581 cgcgagcagg tcgtcgcgcc ggcgcttctg gcgcgcgccc ttgagccgct cctcgagcga
     2641 catgtcgagc agctcgagga tcatgaagtc gttctcgacc cggtcgcgga tcatcggcgg
     2701 gacgaagccg gcgctgtcgc cctcgccgga gccgagcagc tcgacgatgt tcgggtgacc
     2761 ctggacgcgc tccaggatct ccttctccat ctggaaccgc atctggtcct cggcggacac
     2821 gccgcgctgc agcgccttga tcgcgaccgg ccggcgcagc gtggtcttgg actcgaccca
     2881 gcgctcctcg ccgaggtagc acttggcgaa ccggcccgag ccgagctcga tcggcatgcc
     2941 ggtctcgtcg accacgaacg cgtagatccg gctgcgcgag acgtagggcg tcggctgcgc
     3001 gatcacgccg gagacctggg ccacgggagg cccgggcatg gcggtcatcg tcatgccgcc
     3061 gttcatcgcg cccggggcgc cctggtgacc ctggtgcggc gcgtgctgcg cctgcgggac
     3121 catgccgtgc accgacaggc cggggttggt gccgtactgc tgccccgacg gtgacccgat
     3181 gtgcgagagc gacggcgagg ccgacgcgaa ctgaccgggc ggacccggcg ccgggaggga
     3241 gatcgtcggg atggacggcc gcgacgccgg ctgcgtaccg tgggtctggg cgctgggaat
     3301 ctggttgatc gaggtcggat cgccttcgaa gtcgacgaag tcctcggcca gcggagcggc
     3361 cgacggcgag tgcttgcgcg gcgggcttcc cggtggactc gacggcgggg gatgggacat
     3421 gtgcgagctc agcctcggcg tgacttggga atcggccggc cccgagtgta gacgaagtat
     3481 gcttcggatc cggggccaaa gtcgaacagg atcgcgtcga tgcagtcgtc cagcgcaacg
     3541 gacaccagct cgccaccgac cgggaacacg gtgtggccgg cgcccggcgt gccggtggcg
     3601 acccggatgt gaccgatctc ctgcgcgcgg ggcgtgacga gcgcgacatc gctggcctgg
     3661 tcgatctgca cggcgggtga cgggcggccg cccagcggcg cgacgaagca ggtcagtccc
     3721 ttgaggtgca ggacgcgccc gccggtcgga ccggacagaa aggtcacgcg gaccttacta
     3781 ttgtgactgc caatcagatc ggcgacctgg ttgggaacat ggagatagtg cacgcggcgg
     3841 tcgccgacga cctgcggcac gtcggacagc ggcaggcgat gcgggatgcc ctggggcccg
     3901 atcgatggga ccggcacgcg gagcatccac ggcgtcacgg ccgggggcgg cgcccagccg
     3961 ggggaggaca gcggctgggg cggcggcgtg gactccggcc gcggagcggt ttgctgcggg
     4021 g
//
LOCUS       DASVOR010000497         7677 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_4628,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000497 DASVOR010000000
VERSION     DASVOR010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7677
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4628"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VF516_09875"
     CDS             <1..269
                     /locus_tag="VF516_09875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108025.1"
                     /translation="RGGRPDLASSSLAAVRAPTLFIVGGADTWVLQANRDAAARMVAP
                     HRLEVIAGATHLFEEPGALDQVAWLAGSWFIEHFTTRRARARAS"
     gene            203..1360
                     /locus_tag="VF516_09880"
     CDS             203..1360
                     /locus_tag="VF516_09880"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEX8108026.1"
                     /translation="MARRIVVHRAFHDPQGKGESLMITLFVCGDVMLARGIDQILPQP
                     GDPRLYEPSLEDARDYIAIAEDASGPIPRGAPLEYPWGEVLSVLDAARPDARIVNLET
                     AITRRGEPAVEKGIHYRVTPDNAACLRAARIDVCALANNHALDWGTAGLLDTVDTLDH
                     LQIARAGAGRTHAEAVRPALIDLGDRGRVAVFSVGCADAGVPPWWAASEARPGLHALS
                     ELDDDAARKLRHVIEPWRDARTFIVLSIHWGPNWGFAIPREHRLFAHRMIDEAGVDLV
                     HGHSSHHVKGIEVHHGRAILYGCGDLLTDYEGIRGHEAYRGDLGLLYLVTLEDDGALA
                     RLEMVPTRMHRFRVTQAGPKEARWLATTLARSGESLVTSVAIDGQRLSLRW"
     gene            1488..2063
                     /locus_tag="VF516_09885"
     CDS             1488..2063
                     /locus_tag="VF516_09885"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX8108027.1"
                     /translation="MSIPLTITFRDLEPSAAVEDFVRSWGSKLETVYDRIERCHVTIE
                     RPHQHHHQGQRYHVRVTVAVPGPDVEVSRDHALDGAHEDLYVAIRDAFRAARRQLEDH
                     ARRQRADIKAHVEPVHGRVTYLDVEGEWGYLDADGRQVYFHRNSVLDHAALAVGDEVR
                     FTEEPGIKGPQATSVARIGKHGHHERPGSAS"
     gene            complement(2908..3384)
                     /locus_tag="VF516_09890"
     CDS             complement(2908..3384)
                     /locus_tag="VF516_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8108028.1"
                     /translation="MNADSSGRLRLLLAEHHRELETACRALLGHTYGEDPRELSLQYR
                     AFERSTLDHLAAEEELILPAYADHDPDDAAAIRREHATIRRLLFQVGIEVELHIVRAG
                     TVKRLIDVLQAHAAREDASVYRWAQDHLPLSTRRQLFVRIGHALRTLARRSSSVAS"
     gene            complement(3571..4272)
                     /locus_tag="VF516_09895"
     CDS             complement(3571..4272)
                     /locus_tag="VF516_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEX8108029.1"
                     /translation="MLDASSRFREHLAGDRMFADRRSAGRLLAQQLLLYAGTDPIVLG
                     LPRGGVPVADEVARTLGGTLDVWVARKLGVPFQPELGMGAIAEGPAVVLDRSTLELAG
                     ITRAQLLHVARREMTEIRRRVERFRAGRLPPDLRDRTVILVDDGIATGGTMRAAVRAV
                     KKHRPAHLIVAVPVAPPDTVAELRREVDDVVCLHEPEALHAIGLWYADFRQVHDEEVL
                     RILERAAQERPATAP"
     gene            4371..5384
                     /locus_tag="VF516_09900"
     CDS             4371..5384
                     /locus_tag="VF516_09900"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX8108030.1"
                     /translation="MTNLEHLAVFALPPSEALGRAVADDYGLSLAPHELRQFEDGEYK
                     IRPLLGVRGRDVYVVCSLYGDAAESVHDRLCRLLFFVGAVRDAGAARVTIVAPYLCYA
                     RKDRRTNPRDPVTTRYVAQLIEAVGTDRLVTIDVHNRAAYENAFRIGCEHLEAGPLFV
                     ERCRALGDRGLTVVSPDPGGFHRAEALRDALERGGAGTVELAMLGKHRHGGVVRTEAF
                     VGDVAGRIAVIVDDMIVTGTTLVRAADECRRHGAVAVHAMATHGVFTGATGDVLSTPS
                     IDSVIVTDTVAPERVGVGPLRPKLTVVSIAPLLARAISALHHEGSLTQMVSVSLAPMA
                     GAS"
     gene            complement(5390..5629)
                     /locus_tag="VF516_09905"
     CDS             complement(5390..5629)
                     /locus_tag="VF516_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108031.1"
                     /translation="MSVDPAAKLAFLRSQGGLDHAVECIETHMAWVFLSERRAWKLKK
                     SVRLPYLDHSTVEAEQPRWRARADEYLRSIVVGAA"
     gene            complement(5626..5784)
                     /locus_tag="VF516_09910"
     CDS             complement(5626..5784)
                     /locus_tag="VF516_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108032.1"
                     /translation="MARGIATVSLDGHLAERARVELAGTGERILETRLGTTERTRLPA
                     TGGAAERR"
     gene            complement(5805..6512)
                     /locus_tag="VF516_09915"
     CDS             complement(5805..6512)
                     /locus_tag="VF516_09915"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8108033.1"
                     /translation="MAEPHSSLGDEPPVAPRPTGFRSVLVCLDRSRAAEAALPLATHL
                     AKADGASVILFHVLEPAGRGRGSRDPATRHRCLRGRAMASALERDPLPPRSDEPGTAR
                     ELAEAQREIRRTGAAFLDRGDLDALDAAIARHRTRLRARPRDNPSLRIATEWLLDNDY
                     LVRGVFTQLRHELPASFQRRLPRLAGDGRVRAPWWSPARSSACAGDTGRSRSSPASHV
                     HGMVSMPGSEQDSSPCT"
     gene            complement(6915..>7677)
                     /locus_tag="VF516_09920"
     CDS             complement(6915..>7677)
                     /locus_tag="VF516_09920"
                     /inference="COORDINATES: protein motif:HMM:NF012582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108034.1"
                     /translation="SGAAGLMLAVERRATRAVPGEAVEERSLLTALGPFRLPTAGGCT
                     SERERRRAPARGYMDSSPVTTRHTDARLGLASFIDKPLSRLGGSGDYVYQVAAGGQIT
                     NSHTTFVNAIQGLVLGWGDDAPQSQLESLMQLANHAADLGYRSGSQRFVVMMTDAEFH
                     KPGDCTACTHANNGDGIIDPLEDYPSVAQVANALVAANITPIFAVTEPEVAVYEQLTH
                     DLAALGVNQGSVVTLADDSSNIVNAVLTGLKCPAH"
BASE COUNT         1184 a         2625 c         2708 g         1160 t
ORIGIN      
        1 cgcgcggcgg ccggccggat ctggcgagca gctcgctggc ggccgtacgc gcgccgaccc
       61 tgttcatcgt gggcggcgcc gatacctggg tgctgcaagc caaccgcgac gctgctgcga
      121 ggatggtcgc gccgcaccgc ctcgaagtca tcgccggtgc aacccacctg ttcgaggagc
      181 ccggcgcgct cgatcaggtg gcgtggctcg ccggatcgtg gttcatcgag catttcacga
      241 cccgcagggc aagggcgaga gcctcatgat cacgctgttc gtctgcggtg atgtcatgct
      301 cgcgcggggg atcgaccaga tcctgcccca gcccggcgac ccgaggctgt acgaaccgtc
      361 gctggaggac gcccgagact acatcgcgat cgccgaggac gcgtcgggtc cgatcccgcg
      421 cggcgctccc ctggaatatc catggggcga agtactgtcc gtgctcgatg cagcacgacc
      481 cgacgcgcgc atcgtcaatc tcgagaccgc gatcacccgc cgcggcgaac ctgccgtcga
      541 gaagggcatc cactatcgcg tgaccccgga caacgcggcg tgcctccgcg cggcccggat
      601 cgacgtatgt gcgctggcca acaaccacgc cctcgactgg ggcaccgccg ggctgttgga
      661 cacggtggat accctggacc acctccagat cgcccgtgcc ggagctggac gtacgcacgc
      721 cgaggcggtc cgcccggcgc tgatcgatct cggcgatcgc ggccgggtcg ccgtgttctc
      781 ggtcggctgc gccgacgccg gggtgccacc gtggtgggcc gcatcggagg cgcgtcccgg
      841 cctgcacgcg ctgagcgagc tcgacgacga cgctgcccgg aagcttcgcc atgtcatcga
      901 accctggcgc gacgcgcgca cgttcatcgt gctgtcgatc cactggggcc cgaactgggg
      961 cttcgcgatc ccgcgcgagc accggctgtt cgcacaccgg atgatcgacg aggccggggt
     1021 cgatctggtg cacggtcact cctcccacca cgtcaaggga atcgaggtcc accacggccg
     1081 ggcgatcctg tacggctgcg gcgatctgtt gacggactac gagggcattc gcggtcacga
     1141 ggcttaccgc ggtgacctcg ggctgctgta cctggtgacg ctcgaggacg acggtgcact
     1201 ggcgaggctg gagatggtcc cgacccggat gcaccggttc cgggtgaccc aggccggccc
     1261 caaggaagcg cgctggctcg ccacgacgct tgcgcgctcc ggagaatctc tggtcacgtc
     1321 ggtcgcgatc gatggccagc gcctgagctt gcgctggtga tgcggcgcag gcttgccggc
     1381 ctccgtcgcg ggtacgatgg atcttggcac gacggatgct tgaggatcgt atcacggtcg
     1441 ggtcgagccg gactcaaccg gagccgcgac cggctcagag tgctgccatg tccattccgc
     1501 tgacaatcac ctttcgcgat ctcgagccga gcgctgccgt cgaggacttc gttcggagct
     1561 ggggctcgaa gctcgagacg gtctacgatc gcatcgaacg ctgccacgtc accatcgagc
     1621 gcccgcacca gcaccatcac cagggtcagc gctatcacgt tcgcgtgacg gtcgcggttc
     1681 ccggacccga cgtcgaggtc agccgagatc acgcgctcga cggtgcacat gaagatctct
     1741 acgtcgcaat ccgcgatgcg ttccgcgccg cgcgtcggca gctggaggac cacgcgcgcc
     1801 gccagcgcgc cgacatcaag gctcacgtcg aacccgttca cggacgggtc acatacctcg
     1861 acgtcgaagg agagtgggga tacctcgacg ccgacggccg ccaggtctac ttccaccgca
     1921 acagcgtgct cgaccatgcc gcgctcgcgg tcggtgacga agtccggttc acggaggagc
     1981 ccggcatcaa gggaccgcag gcgacctcgg tagctcggat cggcaagcac ggtcaccacg
     2041 agcgccccgg atccgcgagc tgactgccgc cccgcccaag actcgggttc ggcgaaatcg
     2101 gtgcggtcga tcgccggctg agcacggacg cgcggtgacc gttggttcgg tgggccggcg
     2161 agtaccgacg tgccgccgcg gtcggcgagt tgcgcaagat cgcgccgcgc cggttcaacc
     2221 cgaagcgcga ggcgtggtta cccgtgatgc acgtcgagcg cgacggctgg gcgttcaccg
     2281 cgctgtactc gaacacggcg cgcgcacatg atctcgggcg caccgccgac tgggtcattc
     2341 tctactacca tcggccgagc caagccgagg gggcaggcga cgatcgtcac cgagtggcgc
     2401 ggccggctgc gcggcgagcg tgtggtccgt gggcgcgagc tcgagtgcgt ggagtattac
     2461 ggagcggctg ccgcgtagct gcgaggtaca ccgcgagctc gaagtggcag tgtctggttt
     2521 gcgtgaacga gacggcgctg gcgctcgatg gcttcggcgc tcgaagagac gccgtgggcc
     2581 aaacggcacg gtctgggtca atcccggaat gttacttgac gttcccccgg tgatagccgg
     2641 tgtatcggac ctgccccacg ctcgactcga accggcgccc ggatcgacct cgtcccggag
     2701 gatccgcagc aggtcgagcg tggagacgac gccttgcacg gcgccatggt cgtcgaccac
     2761 cggcgcgcca tggagctcgt tctcgacgaa cagccggttc agatgaggac tcgggtctcc
     2821 gggccgaccg tgatgtcgtc cttctccatc agatcggaag cgcgaacgac gggcttgtcc
     2881 agcatggcgt cctccgaggc ggttgcttca gctcgcgacc gacgatgacc gccgggcgag
     2941 cgtgcgtagt gcatgaccga tgcgcacgaa cagctggcgc cgggtggaca gcggcaagtg
     3001 gtcctgcgcc cagcgataca ccgaggcgtc ctcgcgggcc gcatgggcct gcaggacgtc
     3061 gatcaggcgc ttcacagtcc cggcgcgcac gatgtggagc tcgacctcga tgccgacctg
     3121 gaacaggagc cgccggatcg tcgcgtgctc gcgccggatg gcggcggcgt cgtcgggatc
     3181 gtggtcggca taggcaggga ggatcagctc ctcctcggcg gccaggtggt cgagggtcga
     3241 acgctcgaac gcgcggtact ggaggctcag ctcgcgcgga tcctcaccgt acgtatgacc
     3301 gagcagtgcg cggcacgcgg tctcgagctc tcggtgatgc tccgccagca gcagccgcaa
     3361 acggcccgag gaatccgcgt tcatgttcga ggaagcggcc atcatgcccg accaaccgag
     3421 caagctgtac gccagggtcg gtcacggaga agagccgcgg agccgcgcat atcgtgcgcc
     3481 cgatcttcgg atccgtgcaa ctcctgcgac gacggcggcg atgcaagcaa ggacacagtt
     3541 ctcctcgagt cagcccgccg cgccggttta ctacggtgcc gtcgcagggc gctcttgcgc
     3601 ggcgcgttcg aggatccgca ggacctcctc gtcgtgaacc tgacggaagt ccgcgtacca
     3661 caggccgatg gcatggaggg cctctggctc gtgcaggcac acgacgtcgt cgacctcgcg
     3721 gcgcagttcg gcgacggtat cgggcggcgc gaccggaacg gccacgatga ggtgggccgg
     3781 gcggtgcttc ttcacggcgc ggaccgcggc gcgcatcgtg ccgccggtgg cgataccgtc
     3841 atcgacgagg atcaccgtgc gatctcgcag gtccggcggt aggcgcccgg cccggaagcg
     3901 ttcgacccga cgccggatct cggtcatctc tcgccgcgcg acgtgcagga gctgcgcacg
     3961 cgtgatcccc gccagctcca gcgtcgagcg gtcgagcacg acggctggac cctcggcgat
     4021 cgcgcccatg ccgagctccg gctggaaggg tacgccgagc ttgcgcgcga cccatacgtc
     4081 gagcgttccg ccgagagtgc gggcgacctc gtccgccacg gggacgccac cgcgtggcag
     4141 gccaagcacg atgggatcgg tgcccgcata gaggagcagc tgctgtgcga gcagccggcc
     4201 tgcgctgcgc cggtcggcga acatccggtc tcctgcaagg tgttcgcgga atcgggacga
     4261 tgcatccagc atggtccttc acggtgcaac cgggaggccg atatgaatgc cgggcatcag
     4321 gtggcgcgaa cctgcggtcg ggcggcacat ggcttgctgc attgcgagac atgacgaacc
     4381 tggaacacct cgcggtgttt gcgctgcccc ccagcgaggc actgggccgc gccgtggccg
     4441 acgactatgg cctctcgctc gcgccccacg agctccggca gttcgaggac ggcgagtaca
     4501 agatccggcc gctgctcggc gtccgcggcc gcgacgtgta cgtcgtgtgc tcgctgtacg
     4561 gcgatgccgc ggagagcgtc cacgatcggc tgtgccggct gctgttcttc gtcggcgccg
     4621 tgcgcgatgc cggcgccgcc cgggtcacga tcgtcgcgcc atacctgtgc tatgcgcgca
     4681 aggatcgccg caccaatccg cgcgatccgg tgaccacgcg ctacgtcgcg cagctgatcg
     4741 aggcggtcgg caccgatcgc ctcgtcacga tcgatgtcca caaccgcgcc gcctacgaga
     4801 atgctttcag gatcgggtgc gagcatctcg aggccggccc gctgttcgtg gagcgctgcc
     4861 gcgcgctcgg cgaccgcggg ctcacggtgg tctcgccgga ccccggcggg tttcaccgcg
     4921 ccgaggcgtt gcgcgacgcc ctggagcgcg gcggcgccgg gaccgtcgag ctcgcgatgc
     4981 tcggcaagca ccgtcacggc ggcgtggtgc gcaccgaggc gttcgtcggc gatgtcgcgg
     5041 gccggatcgc ggtgatcgtc gacgacatga tcgtgaccgg gacgacgctg gtgcgtgcgg
     5101 ccgacgagtg ccgccggcat ggcgcggtcg cggtccatgc gatggcgacc cacggcgtgt
     5161 tcaccggcgc caccggggac gtgctgtcca caccgtcgat cgatagcgtg atcgtcaccg
     5221 acaccgtggc acccgagcgg gtcggggtcg gcccgctgcg cccgaagctg accgtcgtgt
     5281 cgatcgcgcc gctcctcgcc cgcgcgatca gcgcgctgca ccacgagggt tcattgacgc
     5341 agatggtgag tgtttcactg gccccgatgg ccggcgcctc gtagcgcggt cacgctgcgc
     5401 caacaacgat gctgcggagg tactcgtcgg cccgcgcccg ccagcgcggc tgctcggcct
     5461 cgacggtgct gtggtcgaga tacggcagcc gcaccgactt cttgagcttc cacgcccggc
     5521 gctcgctcag gaacacccag gccatgtggg tctcgatgca ctcgacggcg tgatcgagcc
     5581 cgccctgcga gcgcaggaac gcgagcttgg cggccgggtc gacgctcatc gccgctctgc
     5641 ggccccgccg gtcgcgggca acctggtgcg ctcggtggtt ccgagccggg tctccaggat
     5701 ccgctccccg gtgccggcaa gctcgacccg cgcgcgctcg gccaggtgcc cgtcgaggga
     5761 gaccgtggca atgccgcgcg ccacatgctc ggggtttcgt acgatcacgt gcacggtgag
     5821 ctgtcctgct cggatccagg catcgaaacc atcccatgca cgtgggatgc agggctcgac
     5881 ctcgagcgcc ccgtgtcgcc ggcgcacgcc gaggatcgcg ccggcgacca ccagggcgcg
     5941 cgcacccgcc catcaccggc caggcgcggc aaccggcgct ggaagctcgc agggagctcg
     6001 tggcggagct gggtgaacac gccgcgcacc aggtagtcgt tatcgagcag ccactcggtc
     6061 gcgatccgca gggacgggtt gtcgcgcggc cgggcgcgaa gccgcgtccg gtggcgcgcg
     6121 atcgcggcgt ccagcgcgtc gagatcgccg cgatcgagga acgcggcgcc ggtccgccgg
     6181 atctcgcgct gcgcctctgc cagctcgcgc gcggtccccg gctcgtcgct gcgcggcggg
     6241 agcggatcac gctcgagcgc ggatgccatg gctcgacctc ggaggcagcg gtgccgcgtc
     6301 gccgggtcgc gtgaacctcg accgcgtccg gcgggctcga gcacgtggaa caggatcacg
     6361 ctcgcgccat ccgccttcgc caggtgcgtg gccagcggca gcgcagcttc tgcagcgcgg
     6421 gaccggtcga ggcagaccag caccgaccgg aacccggtcg gccgcggagc gacggggggc
     6481 tcgtcgccga gggacgagtg cggttcggcc atgcatggcc tcgatgcaat gcccatgccc
     6541 cgtaacctcg cgagccagca actccgtgcc cgggattcgc gaacgccttg ctggcgaacc
     6601 tcgatcgaac gcaaatcgcg tgaaccgcat cgggagtcgt tacaccaacg ggcacatcgg
     6661 atgctcgcgc acgagacggg ccggccgcag cgaggctcgt cgtggtgaag acgtggcggg
     6721 ccgggtagcg ataccgaagc aagcgccgtt cgggcacgag ctccaacgac tgcgggccgc
     6781 ggtaggcaac cgcggccggg tgaaacagca cgtccacgtc ggacagcacg gcgaggacgg
     6841 gcgcagcaaa gtgggccggt attccgctca gcacgggcgc gagctcgccg cccgagcgcg
     6901 gcgaagcgct ggcgtcagtg ggccgggcac ttcaagccag tgagcactgc attgacgatg
     6961 ttcgagctgt catcggccag ggtcacgacg ctgccctggt tcacgccgag cgccgcgaga
     7021 tcgtgcgtga gttgctcgta gaccgccacc tccggctcgg tgaccgcgaa gatcggagtg
     7081 atgttcgccg cgacgagcgc gttcgcgacc tgcgccacgg acgggtagtc ctcgagcgga
     7141 tcgatgatcc cgtcgccgtt gttcgcgtgc gtgcacgccg tgcagtcacc cggcttgtgg
     7201 aactccgcgt cggtcatcat gaccacgaac cgctgtgaac cgctgcgata gccgagatcc
     7261 gcggcgtggt tggccagctg catgagggac tcgagctgcg actgcggtgc atcgtcgccc
     7321 cacccgagca cgagaccctg gatcgcattg acgaaggtcg tgtggctatt ggtgatctgg
     7381 ccgccggcgg cgacttggta cacgtaatca ccagacccgc ccagccggga gagaggcttg
     7441 tcgatgaacg aggcgaggcc gaggcgcgcg tcagtatgcc ttgtggtgac cggggatgag
     7501 tccatgtatc cacgggcggg ggctcgccgc ctttcacgct cactggtgca ccctcctgcc
     7561 gtgggcaacc gaaagggacc caaggcggtc agtagggatc gttcctccac cgcctctccg
     7621 gggacggccc gcgtcgctcg ccgctcgacg gccagcatga gacctgccgc cccggag
//
LOCUS       DASVOR010000498         2914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_121406,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000498 DASVOR010000000
VERSION     DASVOR010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2914
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121406"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VF516_09925"
     CDS             complement(<1..855)
                     /locus_tag="VF516_09925"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108035.1"
                     /translation="MTRCARCQRRLPPSGECPVHGRPTGVVTEAATTPDVAPPPGWQI
                     ERHLATGGTAHVFVVRHDSGTLGILKWGRWRERDIYLRFELEAKLLSTVGPAWTAALI
                     DCGVSDGWPYILMELLAGETLAAWMARSGERGGLGEILALLDQLAAGLGAIHAAGIVH
                     RDLKPENIVIGPRGVRLIDFGLARQPQVGGLTQIGAVVGTVHYLAPEQIRGEAVDHRA
                     DLYSFGVVGFEMLCGRPPFVGERRAIEYQHTLCRPPAVREWRSVPEELDALIAACLAK
                     QPETRPQTA"
     gene            942..2279
                     /locus_tag="VF516_09930"
     CDS             942..2279
                     /locus_tag="VF516_09930"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8108036.1"
                     /translation="MSERADPGIAPITAVAGAPELRFPRYRLTVIEGPDRDLAIDGGA
                     AELAIGTAPANTMILTDPAVSRHHIAITPAPRGHLVRDLGSTNGTAINGVAVERAYLA
                     PGAVIAIGRSRLRFDVLGGEDRAALSSDTRWGRALGTSEAMRRIFAVLPRLAESDSTI
                     LLEGETGTGKGLLAEAIHEASPRAAGPFIVLDCGAIPPTLIESELFGHEKGAFTGAAA
                     ARTGGFEAARGGTVFLDEVGELPLDMQPKLLRALEDRVVKRIGGNEPVRLDIRIIAAT
                     NRDLRAEINQGRFRSDLYYRLNTFRLRIPPLRERRDDIALLVAHFHRQLSRGGDPPPA
                     ELIAELTRHDWPGNVRELRAAVERAVLLGDPAVWRALTDDAPPLAEDERFPEGTSFRA
                     AKERAVAAWERDYVRALIARYDGNLSRAARAVRMDRNHLRELLRRHKITADDS"
     gene            2691..>2914
                     /locus_tag="VF516_09935"
     CDS             2691..>2914
                     /locus_tag="VF516_09935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108037.1"
                     /translation="MVDQRTRRLRTTDAEREPRGVQPGEHVDDWIIVRALGGGGFGAV
                     YEARHRATGQRVALKLLHAHLVAAPEIVAR"
BASE COUNT          405 a         1095 c         1025 g          389 t
ORIGIN      
        1 ggcggtctgc ggccgggtct cgggctgctt ggcgaggcac gccgcgatca gcgcgtcgag
       61 ctcctcgggg acgctgcgcc actcgcgcac cgccggcggc cggcacagcg tgtgctggta
      121 ctcgatcgcg cggcgctcgc cgacgaacgg cggccggccg cacagcatct cgaacccgac
      181 cacgccgaac gagtacaggt cggcgcggtg atcgaccgcc tcgccgcgga tctgctcggg
      241 cgccaggtag tgcacggtgc cgaccaccgc gccgatctgg gtcaacccgc cgacctgggg
      301 ctgccgggcg agcccgaagt cgatcagccg gacgccgcgc gggccgatca cgatgttctc
      361 cggcttgagg tcgcggtgga cgatgccggc ggcgtggatc gcgccgagcc cggcggcgag
      421 ctggtcgagc agcgccagga tctcgccgag cccgccgcgc tcgcccgagc gcgccatcca
      481 cgccgcgagg gtctcgccgg cgagcagctc catcaggatg tacggccagc cgtcgctgac
      541 cccgcagtcg atcagcgccg cggtccacgc cgggccgacc gtgctcagca gcttggcctc
      601 gagctcgaac cgcaggtaga tgtcgcgctc gcgccaccgg ccccacttga ggatcccgag
      661 cgtcccggag tcgtggcgga ccacgaacac gtgggcggtg ccgccggtcg ccaggtggcg
      721 ctcgatctgc cagcccggcg gcggtgcgac gtcgggcgtc gtggcggcct cggtgaccac
      781 cccggtcggc cggccgtgga ccgggcattc cccgctcggc ggcaaccggc gttggcagcg
      841 tgcgcagcgc gtcacctgtc gatcacagag tgcaccgatt tgctgcgcga cggaagccgc
      901 cgcgcatcgc atcgtcgtgt cagccgcggt aaaccttccg gatgagcgag cgcgccgacc
      961 cgggtattgc cccgatcaca gcggtggcgg gcgcgcccga gctgcggttc ccgcgctacc
     1021 ggctcaccgt gatcgagggt cccgaccgcg atctcgcgat cgacggcggc gccgccgagc
     1081 tcgcgatcgg caccgcgccg gcgaacacga tgatcctcac cgatccggcg gtgtcgcgcc
     1141 atcacatcgc gatcacgccg gcgccgcgcg gccacctggt ccgcgatctc ggcagcacca
     1201 acggcaccgc gatcaacggc gtcgcggtcg agcgcgccta cctcgcgccg ggcgcggtga
     1261 tcgcgatcgg ccgcagccgg ctccggttcg acgtgctcgg cggcgaggat cgcgcggcgc
     1321 tgtcgtcgga tacccggtgg ggccgcgcgc tcggcaccag cgaggcgatg cggcggatct
     1381 tcgcggtgct gccgcggctc gccgagtccg actcgacgat cctgctcgag ggcgagaccg
     1441 gcaccggcaa gggcctgctc gccgaggcga tccacgaggc cagcccgcgc gcggccggcc
     1501 cgttcatcgt gctcgactgc ggcgcgatcc cgccgaccct gatcgagagc gagctgttcg
     1561 gccacgagaa gggcgcgttc accggcgcgg cggcggcgcg caccggcggg ttcgaggccg
     1621 cgcgcggcgg cacggtgttc ctcgacgagg tcggtgagct gccgctcgac atgcaaccca
     1681 agctgctccg cgcgctcgag gaccgcgtcg tcaagcggat cggcggcaac gagccggtgc
     1741 ggctcgacat ccggatcatc gccgcgacca accgcgacct gcgcgccgag atcaaccagg
     1801 gccggttccg gtccgatctg tactaccggc tcaacacgtt ccggctgcgg atcccgccgc
     1861 tgcgcgaacg ccgcgacgac atcgcgctcc tggtcgcgca cttccaccgc cagctcagcc
     1921 gcggcggcga tccgccgccc gccgagctga tcgccgagct gactcgccac gactggccgg
     1981 gcaacgtgcg cgagctgcgc gccgccgtcg agcgcgccgt gctgctcggc gatcccgcgg
     2041 tgtggcgcgc gctgaccgac gacgccccgc cgctggccga ggacgagcgg ttccccgagg
     2101 ggacctcgtt ccgcgccgcc aaggagcgcg cggtcgcggc ctgggagcgc gactacgtcc
     2161 gggcgctgat cgcgcgctac gacggcaacc tgtcgcgcgc ggcgcgcgcg gtccgcatgg
     2221 atcgcaacca cctgcgcgag ctgctgcgcc gccacaagat caccgccgac gacagctgaa
     2281 gaggtatcgt cgaagcggaa cgaagcgatt ccggtatttc gaccttgtcc tcgacgatga
     2341 ccttgaccac aaccttgacg ttcgacggcg ccgtggacgt cgaccccacc attgaccttg
     2401 cactttgact ccggaggacc gtcgcaggga ggatcaagcc ccgctacgag cgcggcatcg
     2461 gctcgatgac cagcatcgtt gcgatgtgga cgcagatgat ctgaggtcga cgtcgaggtc
     2521 gacgtcaaac cgtccacgtc aaccaccact tcgtcccgaa ccgcctcttt ctctcttcct
     2581 tcgatacctc ttgaccggtg ctggtgggta ccagcgccac cgcggcgtga ccgcgatcgc
     2641 accgcaccgg gtcgacgccg gagccgccgc ggaggtacgc tcggcggcgc atggtcgacc
     2701 agcgcacgcg tcgccttcgc acgaccgacg ccgagcgcga gccgcgcggg gtccagcccg
     2761 gcgagcacgt cgacgactgg atcatcgtcc gcgcgctcgg cggcggcggg ttcggcgcgg
     2821 tctacgaggc gcgccaccgc gcgaccggcc agcgcgtcgc gctcaagctg ctccacgccc
     2881 acctcgtcgc ggcgcccgag atcgtcgcgc ggtt
//
LOCUS       DASVOR010000499         7505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_62967,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000499 DASVOR010000000
VERSION     DASVOR010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7505
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62967"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1136)
                     /gene="gnd"
                     /locus_tag="VF516_09940"
                     /pseudo
     CDS             complement(123..1136)
                     /gene="gnd"
                     /locus_tag="VF516_09940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
     gene            complement(1162..2814)
                     /gene="pgi"
                     /locus_tag="VF516_09945"
     CDS             complement(1162..2814)
                     /gene="pgi"
                     /locus_tag="VF516_09945"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEX8108038.1"
                     /translation="MSALTRSPAWQALIAHQSSLAAATMRELFAADPQRFARMSREVC
                     GLLVDYSKHRATDETWRLLLALAEQSDVAGWRDRMFSGARINVTEHRAVLHVALRNRS
                     NRPILVDGVDVMPQVNAVLARMRDFTERVRSGAWKGATGKSITDVVNIGIGGSDLGPV
                     MVTEALRPYWKPGMRAHFVSNVDGTHLAETVADLDPERTLFIVASKTFTTQETLVNAR
                     SARTWLLDKLRAGGDAVARHFVAVSTNAREVAAFGIDTANMFEFWDWVGGRYSLWSAV
                     GLSIACVIGMDRFEELLDGGHAMDEHFRTAPLADNVPVILGLLGVWYANFWGAETHAI
                     LPYDQDLHRFPAYLQQGDMESNGKSVDRDGHRVDYTTGPVIWGEPGTNGQHAFYQLIH
                     QGTRLIPCDFIAPIETHHPIDRHHEILLANFFAQTEALMKGKTPDEARAELVAARLPE
                     DEIARLVPHKTFPGNRPTTSIVAQKITPRSLGSLIAMYEHKIFVQGIVWNINSFDQWG
                     VELGKQLASRILPELDGDAPVASHDASTNGLINYYKHRRISR"
     gene            3202..5151
                     /locus_tag="VF516_09950"
     CDS             3202..5151
                     /locus_tag="VF516_09950"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: protein motif:HMM:NF018571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEX8108039.1"
                     /translation="MTALTYLQILARRFPDARAVLAELANLHAVLTLPKPTVHVVSDV
                     HGEYVKLRQVINNASGSLRPLFERLFAGRLDGEQIERLLTLTYYPRETWAMLVRRASP
                     ERRRELLHWVAGHAAVAIRDLARHYSLKYVAKIIPDPFDEVFRELVFANELVRQPEFL
                     GRLLQPFLDHDRDGELVAGIAQVIRHLAVGELVVAGDLGDRGPRIDRVIDAIAEQPAV
                     AITWGNHDANWIAATLGHPTAIATVVRLSLRYQRLAQLEDGYGIPLEPVRRLAREAYA
                     DDPADCFRVKGEAEGGPEAAIDLARMHKAISILQFKLEGQLFDRRPEWQLGHRALLRR
                     IDPAAGTVDIDGVRHPLRDTRLPTVDWADPCKLSDGEARCLAELTRAFTSSRTLWSQM
                     AFVVSHGRMSLRRDLCAIFHGCVPVDDAGEPLALVIDGEPRRGKALLDAFERVVHRAF
                     HCALRKGVAEVDELDRDLVFYLWTGPRSPCFGKDRMATFETYLVADKATHEETKNPYF
                     RLIHDAAFCARILAEFGVDPEHGFLVNGHVPVRLEAGETPVKRSGRAITIDGAFAAAY
                     GDKGFSLVLDAQRIYLAQHHHFESAEAAVTRHADIVPTVSDVVVHARLRTVGDTETGD
                     EIRAEIAVLEELLQAFEANILPERT"
     gene            5199..6368
                     /locus_tag="VF516_09955"
     CDS             5199..6368
                     /locus_tag="VF516_09955"
                     /inference="COORDINATES: protein motif:HMM:NF008965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX8108040.1"
                     /translation="MTQTLLQQLAAMTVVVADSGDFNSIKKLKPRDATTNPSLITAAA
                     QMPEYADVVDGALRWAQTEAKGDESRLVALAVDRLSIEFGLRILDIVPGRVSTEVDAR
                     LSFDTAKTVDKARYLIGLYEAAGASRDRVLIKIASTWEGIRAAEILEKQGIHCNLTLL
                     FGMHQAVACADAGVTLISPFVGRILDWYKRAEGRDSYPPAEDPGVVSVTRIYNYFKRH
                     GHRTEIMGASFRNLGEILELAGCDLLTIAPNFLTELGSQTGTLVRKLDPDRARAMDIP
                     RLSFDEAAFRAAHEADKMASDKLAEGIAGFSKAIVALEKLLDERSRALQGKCRAGEAA
                     REFFRAYDLDGDGFITREEWAGSLAVFLALDVDADGKITPEEMAAGLGGAFVLAQ"
     gene            complement(6378..7112)
                     /locus_tag="VF516_09960"
     CDS             complement(6378..7112)
                     /locus_tag="VF516_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108041.1"
                     /translation="MQRLRRLAVPLALLAALAVVIGLVRESAADLENGVFTSRPDHLR
                     MIAPRGWRASEQSTYPGLRLWMARSQPQGQIELTSEAFTRELYCSWPVPCRTSKEILP
                     SKYACALRAKLTKLHMHVGPTEAGPKENEAAGLPSVWFEYDDGRHFLRQAIAISSDRA
                     FSLVLSAPTGDARATHTRAFEQALRTLQLLTPEEARSEAIDAAPPVPGDAPPADAGLI
                     DGAVLDGGVVFESAPAPAINPVGPCK"
BASE COUNT         1074 a         2618 c         2719 g         1094 t
ORIGIN      
        1 cgatgtggca gacgccgcgc tcggcgacgg cggcgcgcgc gatctcggcg aggtcgtcgg
       61 ccgcggcccg gctgagcgcg tcgaggtcgg cgaaccggcg gaccgggggg gcggtcatcg
      121 cgtcaccccc cggtcggacg ctcgacgtgg ccgccgaact ggaagcgcat cgccgacagc
      181 agcttctcgg cgaacgacgc gtcctggcgc gagcggaacc gggtgaacag cgacgcggcg
      241 atcacctcgg cggggacggc ctcctcgatc gcggcctgca cggtccaccg gccctcgccg
      301 gagtcctcga cgtggccgga gaacttgcgg agcgcggggt cgtcgagcag cgcgcgcgcg
      361 gtgaggtcga tcagccacga cgacagcacg ctgccgcgcc gccagagctc ggcgatctcg
      421 gcgaccggca ggtcggcgag gcggtggtct tcggcgacgg tcgggttgcc ggcgccgcgc
      481 atgatgtcga agccctcggc gatcgcctgc atgatgccgt actcgatgcc gttgtggacc
      541 atcttgacga agtgaccgga gccgaccggg ccgcagtgca ggtagccgtc ctcggcggtg
      601 cccggcgacg gcagcttgtc gcggcccggc gtgcgctcgg cgttgccgcg gccgggcgcc
      661 agggtcttga agatcgggtc gagccgctgc accgcggcgg tgtcgccgcc gatcatcagg
      721 cagtagccgc gctcgagacc gaacacgccg ccgctggtgc cggcgtcgac gtaggtgatc
      781 tggcgcggcg ccagcgccct ggcgcgccgg atgtcgtcct tgaagtaggt gttgccgccg
      841 tcgatgatgg tgtcgccggg ctcgagcgcg gcggcgagct ccatcacggt cgcctcggtc
      901 ggcgcgccgg ccgggaccat cacccacacg gcgcgcggcc tggtcaggcc cttgaccagc
      961 gcggcgagcg agtcggcgcc ggtggcgccc tcggcggcga gcgccttcac cgcgtccgga
     1021 ctgcgatcgt agacgacgca ctggtggcca ccgcgcatga gccggcgggc catgttcgcg
     1081 cccatccggc caagtccgat cattccgagc tgcatggttg tctcctgggt tatcccacgc
     1141 gcgcgtcgcg cgtggtccgg gtcatcgtga aattcgccgg tgcttgtagt agttgatcag
     1201 gccgttggtc gatgcgtcgt ggcttgccac cggcgcgtcg ccgtcgagct ccggcaggat
     1261 ccgcgaggcg agctgcttgc cgagctcgac gccccactgg tcgaagctgt tgatgttcca
     1321 gacgatgccc tggacgaaga tcttgtgctc gtacatcgcg atcagcgagc cgagcgagcg
     1381 cggcgtgatc ttctgggcga cgatcgacgt ggtcggccgg ttgccgggga acgtcttgtg
     1441 cggcaccagc cgggcgatct cgtcctcggg gagccgggcg gcgaccagct cggcgcgcgc
     1501 ctcgtcggga gtcttgccct tcatcagcgc ctcggtctgg gcgaagaagt tggcgagcag
     1561 gatctcgtgg tggcggtcga tcgggtggtg ggtctcgatc ggcgcgatga agtcgcacgg
     1621 gatcaggcgc gtgccctggt ggatcagctg gtagaacgcg tgctggccgt tggtgccggg
     1681 ctcgccccag atcaccgggc cggtcgtgta gtcgacgcgg tggccgtcgc ggtcgacgct
     1741 cttgccgttg gactccatgt cgccctgctg caagtacgcc gggaaccggt ggaggtcctg
     1801 gtcgtagggc aggatcgcgt gggtctcggc gccccagaag ttggcgtacc agacgccgag
     1861 caggccgagg atcaccggca cgttgtcggc cagcggcgcg gtgcggaagt gctcgtccat
     1921 cgcgtggccg ccgtcgagca gctcctcgaa gcggtccatg ccgatcacgc acgcgatcga
     1981 cagcccgacc gcgctccaca gcgagtagcg gccgccgacc cagtcccaga actcgaacat
     2041 gttggcggtg tcgatgccga acgcggcgac ctccctggcg ttggtcgaca ccgcgacgaa
     2101 gtgcctggcg accgcgtcgc cgccggcgcg cagcttgtcg agcagccagg tccgcgccga
     2161 gcgcgcgttg accagggtct cctgggtggt gaaggtcttg gacgcgacga tgaacagcgt
     2221 gcgctccggg tcgaggtccg cgacggtctc ggcgaggtgg gtgccgtcga cgttggacac
     2281 gaagtgcgcg cgcatcccgg gcttccagta cggcctcagg gcctcggtca ccatcaccgg
     2341 cccgagatcc gacccgccga tcccgatgtt gacgacgtcg gtgatcgact tgccggttgc
     2401 gcccttccac gcgccgctgc gcacgcgctc ggtgaagtcg cgcatcctgg cgagcacggc
     2461 gttgacctgg ggcatgacgt cgacgccgtc gaccaggatc ggccggttgg agcggttgcg
     2521 cagcgcgacg tggagcaccg cgcggtgctc ggtgacgttg atcctggcgc ccgagaacat
     2581 ccggtcgcgc cagcccgcga cgtcggactg ctcggcgagc gcgagcagga gccgccaggt
     2641 ctcgtcggtc gcgcggtgct tggagtagtc gaccagcagg ccgcagacct cgcgcgacat
     2701 ccgggcgaac cgctgcggat cggcggcgaa cagctcgcgc atcgtggcgg cggcgagcga
     2761 cgattgatga gcgatcaggg cttgccacgc gggggagcgg gtcagggcgg acatggggcc
     2821 agcaaagcac gcttcgtcgc ccggatcgaa cgccgcgcgg tggcgttcgc gccgcgtgcg
     2881 ggcgcgctct cggacggcgc tgcgttcgtc gcggaaatcg gatcgtgctg gccggcgcgc
     2941 gtgcggctcc ggtggtatgg ctcgaggcat gacgcgcgag ccgatggtgc cgatcgggag
     3001 tgctgcgagt ccgcgcgagc gccggcgctc ggccctcggc cctcggcccg cgagatcgcg
     3061 ccggtccctc tccgatcgcg caggcggcgc gatgcgtgag gagcctcctc acggcggcga
     3121 ggtgcggggc gatctgacct gcggccggac cacgctggcc agaccgctcg ggcggccgcc
     3181 cggctcgatc gacgaggggc gatgaccgcg ctgacctacc tccagatcct cgcccggcgg
     3241 ttccccgacg cgcgggcggt gctcgccgag ctcgccaacc tccacgcggt gctgacgctg
     3301 cccaagccca ccgtccacgt ggtcagcgac gtccacggcg agtacgtcaa gctccgccag
     3361 gtgatcaaca acgcctcggg cagcctgcgg ccgctgttcg agcggctgtt cgccggccgg
     3421 ctcgacggcg agcagatcga gcggctgctg acgctgacgt actacccgcg cgagacctgg
     3481 gcgatgctgg tgcggcgggc ctcgccggag cgccggcgcg agctcttgca ctgggtcgcc
     3541 ggccacgcgg cggtcgcgat ccgcgacctc gcgcgccact actcgctcaa gtacgtcgcc
     3601 aagatcatcc ccgatccgtt cgacgaggtg ttccgcgagc tggtgttcgc caacgagctg
     3661 gtccgccagc ccgagttcct cggccggctg ctccagccgt tcctcgacca cgaccgcgac
     3721 ggcgagctgg tcgcggggat cgcgcaggtc atccgccacc tcgcggtcgg cgagctggtg
     3781 gtcgcgggcg acctcggcga ccgcggcccg cggatcgacc gcgtgatcga cgcgatcgcc
     3841 gagcagccgg ccgtcgcgat cacctggggc aaccacgacg cgaactggat cgcggcgacg
     3901 ctcgggcacc cgaccgcgat cgcgaccgtg gtccggctgt cgctgcgcta ccagcggctc
     3961 gcccagctcg aggacggcta cggcatcccg ctcgagccgg tccggcggct ggcgcgcgag
     4021 gcctacgccg atgatcctgc cgactgcttc cgggtcaagg gcgaggccga gggcggcccc
     4081 gaggccgcaa tcgacctggc gcggatgcac aaggcgatct cgatcctgca gttcaagctc
     4141 gagggccagc tgttcgaccg ccggccggag tggcagctcg ggcaccgggc gctgctgcgc
     4201 cggatcgacc ccgcggccgg cacggtcgac atcgacggcg tgcgccaccc gctgcgcgac
     4261 acccggctgc cgaccgtcga ctgggccgat ccgtgcaagc tgtccgacgg cgaggcccgc
     4321 tgcctggccg agctgacccg cgcgttcacg tcgagccgga cgctgtggag ccagatggcg
     4381 ttcgtggtct cgcacggccg gatgtcgctg cgccgcgacc tgtgcgcgat cttccacggc
     4441 tgcgtgccgg tcgacgacgc cggcgagccg ctcgcgctgg tgatcgacgg cgagccgcgg
     4501 cgcggcaagg cgctgctcga cgccttcgag cgcgtggtgc atcgcgcgtt ccactgcgcc
     4561 ctccgcaagg gagtcgccga ggtcgacgag ctcgaccgcg acctcgtgtt ctacctgtgg
     4621 accggcccgc ggtcgccgtg cttcggcaag gaccggatgg cgaccttcga gacctacctg
     4681 gtcgccgaca aggcgaccca cgaggagacc aagaatccct atttccggct gatccacgat
     4741 gccgcgttct gcgcccggat cctggccgag ttcggcgtcg acccggagca cggcttcctc
     4801 gtcaacggcc acgtcccggt ccggctcgag gccggcgaga ccccggtcaa gcgctcgggc
     4861 cgcgcgatca cgatcgacgg cgcgttcgcc gcggcgtacg gcgacaaggg attctcgctc
     4921 gtgctcgacg cccagcggat ctacctggcg cagcaccacc acttcgagag cgccgaggcc
     4981 gcggtgaccc ggcacgccga catcgttccg acggtgtccg acgtcgtggt ccatgcgcgc
     5041 ctgcgcacgg tgggcgacac cgagaccggc gacgagatcc gcgccgagat cgccgtgctg
     5101 gaagagcttc tgcaggcatt cgaggccaat atcctcccag agcgaacgta gaacgaacgc
     5161 agtccgaccc ggaacccggc cagaaccagg aaaccgcgat gacgcaaaca ctcttgcagc
     5221 aactggcggc gatgacggtc gtggtcgccg acagcggcga cttcaactcg atcaagaagc
     5281 tcaagccgcg cgacgcgacg accaacccgt cgctgatcac cgcggcggcg cagatgccgg
     5341 agtacgccga cgtcgtcgac ggcgcgctgc gctgggccca gaccgaggcc aaaggcgacg
     5401 agtcgcggct cgtcgcgctg gcggtcgacc ggctgtcgat cgagttcggc ctgcgcatcc
     5461 tcgacatcgt gccggggcgg gtgtcgaccg aggtcgacgc gcggctgtcg ttcgacaccg
     5521 ccaagaccgt cgacaaggcc cgctacctga tcgggctata cgaggccgcc ggcgcgagcc
     5581 gcgaccgcgt gctgatcaag atcgcctcga cctgggaggg catccgcgcc gccgagatcc
     5641 tcgagaagca gggcatccac tgcaacctga cgctgctgtt cggcatgcac caggcggtcg
     5701 cgtgcgccga cgccggcgtc accctgatca gcccgttcgt cggccggatc ctcgactggt
     5761 acaagcgcgc cgagggccgc gacagctacc cgccggccga ggaccccggc gtggtctctg
     5821 tcacccggat ctacaactac ttcaagcgcc acggccacag gaccgagatc atgggcgcga
     5881 gcttccgcaa cctcggcgag atcctcgagc tcgccggctg cgatctgctc accatcgcgc
     5941 ccaacttcct caccgagctc ggctcccaga ccggcacgct ggtcaggaag ctcgaccccg
     6001 accgggcgcg cgcgatggac atcccgcggc tgtcgttcga cgaggccgcg ttccgcgccg
     6061 ctcacgaggc cgacaagatg gcgagcgaca agctcgccga gggcatcgcc gggttctcca
     6121 aggcgatcgt cgcgctcgag aagctgctcg acgagcgctc ccgcgcgctc cagggcaagt
     6181 gccgggccgg cgaggccgcc cgcgagttct tccgcgccta cgacctcgac ggcgacggct
     6241 tcatcacccg cgaggagtgg gccggctcgc tcgcggtgtt cctggcgctc gacgtcgacg
     6301 ccgacggcaa gatcacgccc gaggagatgg cggccggcct cggcggcgcg ttcgtgctcg
     6361 cgcagtgacg ggccgctcta cttgcacggg ccgaccgggt tgatcgcggg ggccggagcg
     6421 gactcgaaga ccacgccgcc atcgagcacg gcgccgtcga tcaggccggc gtcggccggc
     6481 ggcgcgtcgc cggggacggg cggggcggcg tcgatcgcct cgctgcgggc ctcctcgggg
     6541 gtgagcagct ggagggtgcg cagcgcctgc tcgaacgcgc gggtgtgggt cgcgcgggcg
     6601 tcgccggtcg gcgctgacag caccaggctg aacgcgcggt cggacgagat cgcgatcgcc
     6661 tggcgcagga agtgccggcc gtcgtcgtac tcgaaccaca ccgacggcag gccggcggcc
     6721 tcgttctcct tggggcccgc ctcggtcggc ccgacgtgca tgtgcagctt ggtgagcttg
     6781 gcgcgcagcg cgcacgcgta cttcgacggc aggatctcct tgctggtccg gcacgggacc
     6841 ggccacgagc agtacagctc gcgggtgaag gcctcggacg tcagctcgat ctgaccctgc
     6901 ggctggctgc gcgccatcca cagccggagg ccggggtagg tcgactgctc gctggctcgc
     6961 cagccgcgcg gggcgatcat ccggaggtgg tccgggcggg acgtgaacac cccgttctcc
     7021 aggtcggccg cggactcgcg gaccaggccg atgacgaccg ccagcgcggc gagcagcgcg
     7081 agcgggacgg ccagccggcg aagtcgctgc attcgctgcc aacgctagcg gttctcgccg
     7141 gccggcgcga gtcgctggcc ccaccgcaga cctgaacgga gtcccaggta aaaacttgat
     7201 tcctccagca ttcatcttgt tgacggaatc cattcagacg aatatcgtca caatacttcg
     7261 ttggacacca gtgaatccga ggtcccgcga agatggacga cgcccgtagg gggatcagca
     7321 gacggccgga ctggagcgct gttcgacgga gcagtccgcc gtcgcggtca tcgccgtgcg
     7381 tgcgtcgtcg gtcggttcac gcgagcgaag tcaccgaggg agaagccgtt gctgtcacgt
     7441 catcgaggct ggggcacacg catgtcgtcg accggaacgg tcgtgccgtt tcgcgcgcac
     7501 cggcg
//
LOCUS       DASVOR010000500         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_296957,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000500 DASVOR010000000
VERSION     DASVOR010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_296957"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1254
                     /locus_tag="VF516_09965"
     CDS             <1..1254
                     /locus_tag="VF516_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108042.1"
                     /translation="EASLAKLVPIAISLFADLIGLGGIADKIRGIIEKVQTKVDQAID
                     KLIARVMAMFKGGDKDKDGKDDGKDADPDVPKQLVEPPVTAVGEVNPALSSQLSDSAD
                     GKVLHDTGGDPKTVTKELINSHKDAKYDKASGTLTLPPVHGLAQAGSLEEIGSLVAQQ
                     TGVSKVFLKKNGEQTEIWAAINPQVKIEGLPTDDAILRLAVSRLTIVPFLKQMAAGAA
                     VSVDERGVKGDIDFAKFEKLWGKQSNKDWLKEQFRGAMDGHHEWVPSNFILNVVKNAK
                     DLAEGPKWIDLQHELRNETFTVVFKASASAPEIVDEDGGKRSVPNGHVGAVFYPSVED
                     KNARTKGTGAFHTALRNGFNTGTTVQQVAQNAWNVVTSWVWKGDGDFSPPISKKCFDK
                     NSQQVTPGTQAANYQKIETEFKKFL"
     gene            1261..1884
                     /locus_tag="VF516_09970"
     CDS             1261..1884
                     /locus_tag="VF516_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108043.1"
                     /translation="MADPFDFDAHRTRGTSREATDWVQQSTLPDDHKALLLGFIARFP
                     TLTFYKDDAALLDHIEQRDQLTLPRWFRDQRQVLAFVAPPALVRFDDFDHLGPRSDTL
                     DRIWYAIELDGYRDDEQRELFLDQASSYPIGQWPHTDRSFLAIHTADPDDPRIHEFAA
                     QDLLDNVLDGGAARDSVYPAFDSYARMLSHIIEIKGAGDPVSAAVTP"
BASE COUNT          382 a          626 c          607 g          279 t
ORIGIN      
        1 gaggcctcgc tcgccaagct ggtcccgatc gcgatcagcc tgttcgccga cctgatcggc
       61 ctgggcggca tcgccgacaa gatccgcggc atcatcgaga aggtccagac caaggtcgac
      121 caggcgatcg acaagctgat cgcccgcgtc atggcgatgt tcaagggcgg ggacaaggac
      181 aaggacggca aggacgacgg caaggacgcc gatccggacg ttcccaagca gctggtcgag
      241 ccgccggtca ccgcggtcgg cgaggtcaac ccggcgctgt cctcgcagct cagcgactcc
      301 gccgatggca aggtgctcca cgacaccggg ggcgatccca agacggtgac caaggagctg
      361 atcaacagcc acaaggacgc gaagtacgac aaggcatcgg gcacgctgac gctgcccccg
      421 gtccatggtc tggctcaggc cggctccctg gaggagatcg gctcactggt cgcccagcag
      481 accggcgtgt ccaaggtgtt cctgaagaag aacggcgagc agaccgagat ctgggctgcg
      541 atcaacccgc aggtcaagat cgaagggctg ccgaccgacg acgcgatcct ccggctcgcg
      601 gtgtccaggt tgaccatcgt gccgttcctc aagcagatgg ccgccggcgc cgcggtctcg
      661 gtcgacgaac gcggcgtcaa gggcgacatc gacttcgcca agttcgagaa gctgtggggc
      721 aagcagagca acaaggactg gctcaaggag cagttccgcg gggcgatgga cggccaccac
      781 gagtgggtac cgagcaactt catcctcaat gtcgtgaaga acgccaagga cctggccgag
      841 ggtccgaagt ggatcgacct gcagcacgag ctgcgcaacg agacgttcac ggtcgtgttc
      901 aaggcgtccg cctcggcgcc cgagatcgtc gacgaggacg gcggcaagcg cagcgtgccc
      961 aatggccacg tcggcgccgt gttctacccc tcggtggagg acaagaacgc caggaccaag
     1021 ggcaccggcg cattccacac ggcgctgcgc aacgggttca acaccggcac cacggtccag
     1081 caggtcgcgc agaacgcctg gaacgtggtc accagctggg tgtggaaagg cgacggcgac
     1141 ttctcgccgc ccatcagtaa gaaatgcttt gacaagaaca gccagcaggt caccccgggc
     1201 acgcaggctg cgaactacca gaagatcgag acggagttca agaagttcct ataatcgggc
     1261 atggccgacc cgttcgactt cgatgcccac cggacccgcg gcacgtcccg cgaggccacg
     1321 gactgggtcc agcagtcaac gctccccgat gaccacaagg cgcttctgct gggattcatc
     1381 gcccggtttc cgaccctgac gttctacaag gacgatgcgg ccttgctcga ccacatcgag
     1441 caacgtgatc agctgacact gccgcgctgg tttcgcgacc agcgccaggt gctcgcgttc
     1501 gtggcaccgc cggccctggt gcggttcgac gacttcgacc atctcggccc tcgatccgac
     1561 acgctggacc ggatctggta cgcgatcgag ctggatggct atcgcgatga cgagcaacgc
     1621 gagctgttcc tcgatcaggc cagcagctat ccgatcgggc aatggccgca caccgaccga
     1681 tcgtttctcg cgatccacac ggccgacccg gacgaccctc ggatccacga gttcgcggcg
     1741 caggatctgc tcgacaacgt gctcgacggc ggagccgcgc gcgactccgt gtatccggcg
     1801 ttcgactcct atgctcgcat gctgtcgcat atcatcgaga tcaagggcgc cggcgacccg
     1861 gtgagcgcgg ccgtcacgcc ctgacggcgt cacg
//
LOCUS       DASVOR010000501         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_33619,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000501 DASVOR010000000
VERSION     DASVOR010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33619"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF516_09975"
     CDS             <1..199
                     /locus_tag="VF516_09975"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8108044.1"
                     /translation="MQFAEKIRKLVEVAEFKFEDAIIPVTVSIGVASLRGEIEDALEF
                     IKQADSNLFAAKEGGRNRVVG"
     gene            complement(201..1580)
                     /locus_tag="VF516_09980"
     CDS             complement(201..1580)
                     /locus_tag="VF516_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108045.1"
                     /translation="MVRAAAAAALAAAGALAGCTLITDSFLTNDFSGDPFPTRIDDRS
                     GALLVGVQPLDGSDPRAAVLDLLSPFTLSDPGTIGDPSLTTVDLMLLGKNVDGMLTLP
                     RARFPQAQLISLHPCDVPAGADPKQLPPCMVGTPDDPRPFTAVVGADVLAGDAIRLRL
                     ASDPSTARSTSDIFVLPDIGGSDRGRTLSCEAVFDAPYRGGGTLVISGTELPFGNRRI
                     TLSACLGQDPDPGMQQTPTDHGADALFVMSTSIGISMLDQSAYRRYQNAVGVASAPNV
                     DTLPTGAVYLPSGLVMGRLATINRMALVGASISNGLSPCRHIYAHHMLADGPVAQNPS
                     MDCNTLTGQNCPCKDGSSFCAVPAIVELTPPASLPVLVVPDTEPTLQALRTELRPDQP
                     EVDGLLGTDALRGAEIDVDYPHDRLIARCTQPGCLVRPQLTQSGDRCQINRCIYGSAD
                     TVGCPQPQP"
     gene            complement(1574..2077)
                     /locus_tag="VF516_09985"
     CDS             complement(1574..2077)
                     /locus_tag="VF516_09985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108046.1"
                     /translation="MDDVVFEPLAGPAHQAWDDPAGVWAAPIIAGEAPGARPDADPEV
                     ERLRIRGGFLRYGVDVDEDHFPFETPLGRFLDYGKGCYVGQEPVFRVHAQGNAARMLR
                     GLTIEGSAPIAAGTALAAPAKGTITSSIADGDATRALAYLHRSCWEPGGTVEVEGRRA
                     TVHELPW"
     gene            complement(2075..2335)
                     /locus_tag="VF516_09990"
                     /pseudo
     CDS             complement(2075..2335)
                     /locus_tag="VF516_09990"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          407 a         1019 c         1088 g          408 t
ORIGIN      
        1 gatgcagttc gccgagaaga tccgcaagct ggtcgaggtc gccgagttca agttcgagga
       61 cgcgatcatc ccggtgacgg tgtcgatcgg ggtcgccagc ctgcgcggcg agatcgagga
      121 cgcgctcgag ttcatcaagc aggccgactc caacctgttc gcggccaagg aaggcggccg
      181 caaccgcgtg gtcggctagc tcacggctgc ggctgcgggc agccgaccgt gtcggccgag
      241 ccgtagatgc aacggttgat ctggcagcga tcgccggact gggtcagctg cggccgcacc
      301 aggcagcccg gctgggtgca ccgcgcgatc agccgatcgt gcgggtagtc gacgtcgatc
      361 tcggcgccgc gcagcgcatc ggtgccgagc aggccgtcga cctcgggctg atccggccgc
      421 agctcggtgc gcagcgcctg cagcgtcggc tcggtgtcgg gcacgacgag gaccgggagg
      481 ctcgccggcg gggtcagctc gacgatcgcg ggcaccgcgc agaacgagct gccgtccttg
      541 cacgggcagt tctggccggt cagcgtgttg cagtccatgc tggggttctg cgccaccggc
      601 ccgtcggcca gcatgtggtg cgcgtagatg tgccggcacg gcgacaggcc attcgagatc
      661 gacgcgccga ccagcgccat ccggttgatc gtggcgagcc ggcccatgac caggcccgac
      721 ggcaggtaca ccgcgccggt cggcagcgtg tcgacgttcg gggcgctggc caccccgacg
      781 gcgttctggt agcgccggta cgccgactgg tcgagcatcg agatcccgat gctggtcgac
      841 atcacgaaca gcgcgtcggc gccgtgatcg gtcggggtct gctgcatgcc gggatcggga
      901 tcctggccca ggcaggccga cagcgtgatc cggcggttgc cgaacggcag ctccgtgccc
      961 gagatcacca gcgtgccgcc gccgcgatac ggcgcatcga acaccgcctc gcacgacagc
     1021 gtgcggccgc gatcgctgcc gccgatgtcg gggagcacga agatgtcgct ggtcgagcgc
     1081 gccgtcgacg ggtcgctggc gagccgcagc cggatcgcgt cgccggccag gacgtcggcg
     1141 ccgaccacgg cggtgaacgg cctcggatcg tcgggggtgc cgaccatgca cggcggcagc
     1201 tgcttcggat cggcgccggc ggggacgtcg cagggatgca gcgagatcag ctgggcctgg
     1261 gggaaccggg cgcgcggcag ggtgagcatg ccgtcgacgt tcttgccgag cagcatcagg
     1321 tcgaccgtcg tcagcgacgg atcgccgatc gtgcccggat cgctcagcgt gaacggcgac
     1381 agcaggtcga gcacggcggc gcgcgggtcg gacccgtcga gcggctgcac gccgaccagc
     1441 agcgcgccgc tgcggtcgtc gatccgggtc gggaacggat cgcccgagaa gtcgttggtc
     1501 aggaagctgt cggtgatcag cgtgcagccg gccagcgccc cggccgcggc gagcgcggcg
     1561 gcggccgcgg ccctcaccat ggcagctcgt gcaccgtggc ccggcgaccc tcgacctcga
     1621 ccgtgccgcc gggctcccag cagctgcggt gcaggtaggc gagcgcgcgg gtcgcgtcac
     1681 cgtcggcgat cgacgacgtg atcgtgccct tggccggggc ggcgagcgcg gtccccgcgg
     1741 cgatcggcgc gctgccctcg atcgtcaggc cgcgcagcat gcgggcggcg ttgccctggg
     1801 cgtggacccg gaacaccggc tcctggccga cgtagcagcc cttgccgtag tcgaggaacc
     1861 ggccgagcgg ggtctcgaac gggaagtgat cctcgtcgac gtcgaccccg tagcgcagga
     1921 agccgccgcg gatccgcagg cgctcgacct cgggatcggc gtcggggcgg gctcccggcg
     1981 cctcgccggc gatgatcggc gccgcccaca ccccggcggg atcgtcccac gcctggtgcg
     2041 cgggcccggc gagcggctcg aacaccacgt cgtccatcac cgcgtagcgc tcgagcagcg
     2101 cgcgggtctt gtcggtgagc cccggctcgc acgccaggac ataggcgtcg ccggcgcggg
     2161 cgacgtcgat caccgacagc acgcggccct tggggctgag gatcgcgccc cagccgtgcc
     2221 cgccgtcggc cagcgcctcg acgttgaccg tggtcaggcc ctgcaggaac cgcgtccggt
     2281 cgccgccggt gacccgcagc cggccccagc cggagcgatc gacggggatc atgacggcag
     2341 ctccgcgcgc ccggcggcgc gcaggcgagt ctcggcggga gtcatcgggg cggtcagcgg
     2401 gaacctcccg ccaaccttac ttcttcttcg atggcgcggc aggcttcttg gcggccttgg
     2461 cggcatcgat cttgcgatgg atccgcgcct tgagcttggc ctggctcttc cggcgcttca
     2521 tcttcggtgt acggcgattg tctccacgtc ccatgggggc gtcgtatatc acatcggccc
     2581 ggccgaggtg cacctgggag cgccagctcc gtgacggcgt gcgacccagg gtacccccag
     2641 atccgggtcc gggcgaacgt cgagcgcgct gccaccccga cgcgctccag gcgctgcacg
     2701 accacgtcgt gcgcacttcg ccgatctgcg gcacggtcgc gcccggtggc agtcaccgcc
     2761 gagcggatcg cgcggtagcc gcagcgctcc ctcaggtgaa gatgtcgagc ggcaggaacg
     2821 ccgataccac gacgttgggg acgtcgacga tctcgctgat cgcgaggtcg accgcgacgc
     2881 tgcagatcgc gtacgcctgc tggcggttga acccgcgctc gt
//
LOCUS       DASVOR010000502        12267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_560037,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000502 DASVOR010000000
VERSION     DASVOR010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560037"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VF516_09995"
     CDS             complement(<1..470)
                     /locus_tag="VF516_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108047.1"
                     /translation="MRALDLPTPRICQIERTANLAGTAVCDVPEPPWIAAMAAPSFSK
                     VEHDAARSALDLIGSLWAVLPELRDHGPQGTNQIERDVIGEEHGFSWVFSSGRERPLP
                     TTSAAGLRPSRPIRATTRDERPHRRTWSSQRTSSRRRSEPPGRAGVRREGVRGRA"
     gene            complement(706..1704)
                     /locus_tag="VF516_10000"
     CDS             complement(706..1704)
                     /locus_tag="VF516_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8108048.1"
                     /translation="MHRDIVIDKIGPASALTLREITARPPGDDEIAIDVAYSGINFAD
                     IHMRLGLYPDAPKRPFVPGYEVSGKIAAVGKNVRDLQPGDPVLAGTFFGGYASTVTIP
                     AQQVLKLPAAYDLAAGAALPVACFTAQLAVVEMARVRRGDRVLIESATGGVGVIAIQM
                     ARHLGVEVTGLTTSPHKKSLITELGATAYTVDEFRADPKLTGYDFILNASGGASIEWQ
                     RARLGLTGRMVCIGMSSLVKDGKRSLLRAAAAVIRTPRISVLKLFDASTGIFGLNSLH
                     VLQDPAWAARLTRSLAAVEQLGVKPHIGKVFPAAEVAAAHAFLETKQATGKVLLQW"
     gene            1751..2017
                     /locus_tag="VF516_10005"
     CDS             1751..2017
                     /locus_tag="VF516_10005"
                     /inference="COORDINATES: protein motif:HMM:NF019009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HEX8108049.1"
                     /translation="MRNPPYDVKATKQTVSLTINSDLYAQAKKLGINASQVAEEALAQ
                     QVARRKVEQLKAEIRQDLEAVNSYVAKHGSFAEMVREHYHSEED"
     gene            2018..2332
                     /locus_tag="VF516_10010"
     CDS             2018..2332
                     /locus_tag="VF516_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HEX8108050.1"
                     /translation="MAQFDVFVNPVVRARRTYPYVAILQSDLAETGGERIVAPLAPRA
                     RVTGTAGRLTPHVRVLEVEHVLIVPAMAAIAAGDLRELRGQLTRHREAIVAALDYLFL
                     GV"
     gene            complement(2355..3005)
                     /gene="yfcF"
                     /locus_tag="VF516_10015"
     CDS             complement(2355..3005)
                     /gene="yfcF"
                     /locus_tag="VF516_10015"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF011693.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HEX8108051.1"
                     /translation="MSGSATPQLTLFAESSWMSPWVFHAMVALEEKQLPYKLEVVSLP
                     IPDAIRADLQRRALIAKVPVLVHGDTWISESLAISEYLAETFRAPDHPRLFPENLAER
                     ARARQVMSFLRTSLFALREARPTSTVFGRPNPRPLSDAAREEAAELERLALALLLDDR
                     PAMFGTWCIADIDLALALMRLVANQDPLDRRLVAYALAQFDRKSVHRFVAHLPTTR"
     gene            complement(3063..3518)
                     /locus_tag="VF516_10020"
     CDS             complement(3063..3518)
                     /locus_tag="VF516_10020"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8108052.1"
                     /translation="MVDSDVVTMLNAGRGGFEVAIGLQFTRASTEEVACTVPVGPHLT
                     QPYGLVHGGVYATIVETLASVGAALFAMPRGQTTVGLENTTSFLRAVRSGTLTGTARP
                     LHRGRATQVWQVEVHGDDGKLAATGRVRMLCLEPGATVAGEVVARKPAG"
     gene            3627..4034
                     /locus_tag="VF516_10025"
     CDS             3627..4034
                     /locus_tag="VF516_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HEX8108053.1"
                     /translation="MPLNVNLLRQSFEVVATANPRFVSRFYEILFERHPPARALFPQN
                     GMARQAEMLTQALVAVLDHLEDAPWLVDTLGALGAKHVGYGVTREMYDWVGTSLLATL
                     AEVAGPAWTPELQAAWAEAYGAIVSLMLARAGT"
     gene            complement(4068..5744)
                     /locus_tag="VF516_10030"
     CDS             complement(4068..5744)
                     /locus_tag="VF516_10030"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF006006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8108054.1"
                     /translation="MRIFSQLFRHRVTLAATLLLGFALGAGTTTAGSNASRHGDPMLR
                     GIDLERATIPQLQDAMDHGRISSVLLTAFYLDRIEDLNPELHAVIATSPTALIEAAFS
                     DIRRATHHLRGPMDGIPVLLKDNVDTENVRATAGSEALLGARGRDAFLVSRLRDAGAV
                     ILAKANLSEWANFRSFLSSSGWSGVGGQTNNPYVLDRNPCGSSSGSGAGIAANLAVVA
                     IGTETDGSIVCPSGQNGIVGIKPTLGLISRTGVVPISAEQDTAGPMARSVTDAAVMLS
                     ALTGIDPQDPATAASAGHIARDYTRFLDRNALRGKRIGVWRTVTGGDPATDAVFAQGI
                     AALQAAGATTVDVDLPLQDVVGNNEFPALLNEFKHDMKAYLSSVGGNHPADLQGLIDF
                     DLAHADTELQFFLQEIFDLSQTFSGDLTDPDYLTQRATATGSARQSIDQTMADLHLDA
                     IAAPTNSPAWVTTLGQGDNFVFGSSAPAAVAGYPNISVPMGFSGELPVGVSFFASRFE
                     EPKLIGLAFAFEQATHARRPPQFLPTLPATGAPISKARPQTITSLPPPLW"
     gene            complement(6064..7779)
                     /locus_tag="VF516_10035"
     CDS             complement(6064..7779)
                     /locus_tag="VF516_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108055.1"
                     /translation="MVAGALAACGDNGDHVQILLFQASPDVIESGQSTTLVFVVEPAN
                     AKVSITGMGDLTGKSQVSVAPTDTTSYQLTATSGKAVTNQTVRVTVGATSAAAIKVQP
                     ATKTPTAGAPVAVTLTVLGPDGNPAMGFRGTVHVSSTDANAVLPADVTFMAGEAGVKQ
                     VMVTLKAAGLSTLTATDVTGKANASGSASLTVQPAAAHSYQLDPLPGSATAGQALTLT
                     VKVLDVFGNVATAYGGKLHLTSADSTDMLPSDGTFSAGVLAVSLTFIKTGNHAVQIND
                     VALALPSASSSSVAVGPGAPKVVLGLPLNANSGFPVNVDVVIRDLFENAIPNYAGTVS
                     FATTDKGTGATTPASITFSGSEGGVSTTTVTFVTMGTQTLSATGTAASGPQALGSAVA
                     QVHGLVYTAPTTGKVRLVANAASNPQTIQLDLVASERLTVSSFFGGPGSFTAGMNLPL
                     DTTRVGAGTPLFTRGLALPAGTGVDASAGVIGSDHVLYTGVSRKRTPNNANGTNPVSI
                     QDTQVNAGDVFYSVKLKLTQNGPPGTVFDGAQPTAMFRAAVRDQFGDDFVGQVDFGVG
                     KLEIR"
     gene            complement(7806..10976)
                     /locus_tag="VF516_10040"
     CDS             complement(7806..10976)
                     /locus_tag="VF516_10040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108056.1"
                     /translation="MKKAIYGSVVVTVGLTTTVIVAHAVNSPSQQQQLQNQRDVTIAA
                     LKDAIARGDDGIGPLPINYVEDTSDVVADDALVRSLWLRLTNGPASQAGIVNLMHETA
                     VQQQREASYSTAARQITDAGGPSPTPTTPLQWVQLGPQSALSEWNGSYYDGLDSGRVA
                     TIRTDPQNPATVYIGAIGGGIWKTPDITVTQPVWQPLTNTLGTMFIGSFDIDPTSSLI
                     IHAGLGDYWEGNPGGVMVTSTDGGATWSAPHLLTTTLNSTAIHAVNTRTVRIDPNNPN
                     NILVASDVGLFRSTDGGAHYAPIDLPNTTAYGNDLEGTFSVVYTGVSAATGLSTFLVS
                     GNYACPGTYPPSFNQPTTGFFVTQCPGMPTGQGNLGDIWTSNDGGATWSSARVAGLFP
                     ATFELAGTPPAPVPSEMGRINLTAIPGSPNSDSAIIYALAGDQNGAHTIAVLKSTNGG
                     TSWFSVAQGALTSPTNPTPGATGGDCLNLDIGHGQSQYDLAVAVDPGNPNNVMIGGNL
                     CGARTVDGGTTWQIASDWLAFGGDEGSLPYVHADWHNSHIIRVNGQPIALASSDGGIF
                     ASFDLFSAARGSDVHFFDANIGLDTLLPYSVGSGDPVFGTAKYVLTGLQDNGTRIRVS
                     QSEFYLSNFPKAWNQIQGGDGFGAAVSNDATGGNVTTWGVANGGRIACRAGAGVECTR
                     ATHVVNDSEIRSYFRVTPTLPPGDTNNGFAVRYAPLYDAAGSVVSNSNFNLWKLTPGA
                     GDTVSIVRLVTSPAPPNPGGYVGCGSTAARTIRAGGPQASPFTYTVNGVASRILGLPL
                     AGGCYAVVVDPGRADGVVSVVTANTIPQIGPEQVQNTASITFPKDPTHLGGTDITKTY
                     VVSSIGDFVTAPVTNPPTPISAATGHVFLTTDGGTTWTPLHGNGTGFDLPNVRVYVIR
                     FDPSDVTDQTLWAGTDLGLYRSTDQGKTWARYGSNLPMVRVQDLFLSLNGSLIRAAMY
                     GRGVWEIYPRSDGANGALGLGDFDKNGIIDFRDLGNLTNRLTVTAAGAEVPLYDSEMN
                     LSETGATTTLDDNDLSALLAKFGGAP"
     gene            11536..>12267
                     /locus_tag="VF516_10045"
     CDS             11536..>12267
                     /locus_tag="VF516_10045"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8108057.1"
                     /translation="MPLRVVIRLMVLESLRALARNKVRSGLAMLGIAVAVAIVITVIA
                     LGRASIAASEAELDKLGDNLVWVEAGSRNAAGVRTGSHGMTTLTARDADAIRSEIPLI
                     KDVSENVDGGFQVVARDHNWQTRWRGVAPSYQAIKRWDIARGRFFDDDQTEHAARVIV
                     IGETVRRELFPDSEPIGEIVRIRGLGFEVIGVLAPKGQSATGQDQDDTVMMPWTTAMK
                     QIVGKDQTWLDDILCSARATDAIAPA"
BASE COUNT         1818 a         3990 c         4543 g         1916 t
ORIGIN      
        1 gcccgtccgc gcacgccctc gcgccgaacg ccagcgcgtc cgggcggctc cgaccgccgc
       61 cgcgaagatg tgcgctggga agaccacgtg cgccggtgag ggcgttcgtc acgggtcgtg
      121 gcgcggatcg gtcgcgacgg gcggagccca gccgcggagg tcgtcggcag agggcgttcc
      181 cttccagaac tgaacaccca ggagaagcca tgttcctcgc ctatgacgtc gcgctcgatc
      241 tggttcgtgc cctgcggccc gtggtcgcgc agctccggaa gtacagccca gaggctgccg
      301 atcaagtcga gcgcgctgcg agcagcatcg tgctcaacct tgctgaagga gggcgccgcc
      361 atggccgcga tccacggcgg ttctgggaca tcgcacacgg cagtgccggc gagattcgcg
      421 gtgcgctcga tctggcagat gcgtggggtt ggcagatcga gagcacgcat gcgcgtgcgc
      481 tgctcgatcg cgagctgagc cttttgtggg gacttacccg ccgcagccgc gccaaggacg
      541 catcgctccc agcgtcagga gccaggacgc gaccgcggtg acccggtgcg tgcggcggac
      601 acagcagcgc ccgcagccca agccagcgga ctgacagagc gtggcgtgcg gtggccgcag
      661 atgccccgcg gtcgaacccg aggggccggc gtcagcgcgg gcccgctacc actgcaggag
      721 gaccttgccg gtcgcctgct tggtctcgag gaacgcgtga gcggcggcga cctcggcggc
      781 cgggaacacc ttgccgatgt gcggcttgac gccgagctgc tcgaccgcgg ccagcgaccg
      841 ggtcagccgg gcggcccacg ccggatcctg cagcacgtgg agcgagttga gcccgaagat
      901 cccggtgctg gcgtcgaaca gcttgagcac cgagatccgc ggcgtgcgga tcacggcggc
      961 ggcggcccgc agcaggctgc gcttgccgtc cttgaccagc gacgacatgc cgatgcagac
     1021 catgcggccg gtcaggccga gccgggcgcg ctgccactcg atgctcgcgc cgccgctcgc
     1081 gttgaggatg aagtcgtagc cggtgagctt gggatcggcg cggaactcgt cgacggtgta
     1141 ggccgtggcg ccgagctcgg tgatcagcga cttcttgtgc gggctggtcg tcagcccggt
     1201 gacctcgacc ccgaggtggc gggccatctg gatcgcgatc acgccgacgc cgccggtggc
     1261 gctctcgatg aggacgcggt cgccgcggcg cacccgcgcc atctcgacca ccgcgagctg
     1321 cgcggtgaag caggcgaccg gcagcgccgc gccggcggcg aggtcgtagg cggcgggtag
     1381 cttgagcacc tgctgcgccg ggatggtgac ggtcgaggcg tagccgccga agaacgtgcc
     1441 ggcgagcacc gggtcgccgg gctggaggtc gcggacgttc ttgccgaccg cggcgatctt
     1501 gccgctgacc tcgtagccgg ggacgaacgg ccgcttcggc gcgtcggggt acaggccgag
     1561 ccgcatgtgg atgtcggcga agttgatccc cgagtacgcg acgtcgatcg cgatctcgtc
     1621 gtcgccgggg ggacgcgcgg tgatctcgcg cagggtcagc gccgaggcgg ggccgatctt
     1681 gtcgatgacg atgtcgcggt gcatgggcag cgcatgttgc ccatggctct gacccgtgta
     1741 aagtacacgt gtgaggaacc cgccctacga cgtcaaggct accaagcaga ccgtgagctt
     1801 gacgatcaac tcggatctct atgcccaggc gaagaagctc ggcatcaatg cctcgcaggt
     1861 cgcagaggag gcgttggcgc agcaggtcgc ccggcgcaag gtggaacagc tcaaggccga
     1921 gatccgccag gatctcgaag ccgtgaactc gtacgtggcg aagcacgggt catttgccga
     1981 gatggtgcgg gagcactacc acagcgaaga ggactgagtg gcgcagttcg acgtcttcgt
     2041 caacccggtc gtacgggcgc ggcgcacgta tccgtacgtg gcgatcctgc aatcggatct
     2101 cgccgagacg ggcggagaac ggatcgtcgc gccgctggcg ccgagagcgc gggtgacagg
     2161 gaccgccgga cgcctgacgc ctcatgtgcg ggtgctggag gtcgagcacg tgctgatcgt
     2221 cccggcaatg gcggccatcg ccgccggaga tctgcgcgag cttcgcggac agctcacccg
     2281 tcatcgcgag gcgatcgtgg ccgcgctcga ctacctgttc ctgggcgtat gaggtgcatc
     2341 tcggctgggc gtggtcaccg cgtggtcggc aggtgggcga cgaagcggtg gacgctcttg
     2401 cggtcgaact gggccagggc gtaggcgacc agccggcgat cgagcgggtc ctggttggcg
     2461 accaggcgca tcagggcgag cgcgaggtcg atgtcggcga tgcaccaggt gccgaacatc
     2521 gccgggcgat cgtcgaggag cagggcgagc gcgaggcgct cgagctcggc ggcttcctcg
     2581 cgggcggcgt cggacagcgg ccgcgggttg ggccggccga acaccgtgga cgtcggccgg
     2641 gcctcgcgca gcgcgaacag gctggtgcgc aggaacgaca tgacctggcg ggcgcgggcg
     2701 cgctcggcga ggttttccgg gaacagccgc gggtgatcgg gcgcccggaa ggtctcggcg
     2761 aggtactccg agatcgcgag cgactcggag atccaggtgt cgccgtggac caggacgggg
     2821 accttggcga tcagcgcgcg gcgctggagg tcggcgcgga tcgcgtcggg gatcggcagc
     2881 gagacgacct cgagcttgta cggcagctgc ttctcctcga gcgcgaccat cgcgtggaac
     2941 acccaggggg acatccagct cgactcggcg aacagcgtga gctgcggggt ggcggatccg
     3001 gacatggccg cgaaggtagc aagccgggcc gggtgcgggt gccgcgaaac gcccctggcc
     3061 ggtcacccgg cgggcttgcg cgccacgacc tcgcccgcga ccgtggctcc cggctccagg
     3121 cacagcatgc gcacgcggcc ggtggcggcg agcttgccgt cgtcgccgtg gacctcgacc
     3181 tgccagacct gggtcgcgcg gccgcggtgc agcggacggg cggtgccggt cagcgtaccg
     3241 ctgcgcacgg cgcgcaggaa cgaggtcgtg ttctccagcc cgacggtggt ctggcccctg
     3301 ggcatcgcga acagcgcggc gccgacgctg gccagggtct cgacgatcgt cgcgtacacc
     3361 ccgccgtgga ccaggccgta cggctgggtc aggtgcgggc cgaccggcac ggtgcacgcc
     3421 acctcctcgg tggaagcccg agtgaactgc agtccgatcg cgacttcgaa tccgccgcgt
     3481 ccggcgttga gcatcgtgac gacatcgcta tcgaccatgc ctgcaggcta aaccgagccg
     3541 gatacgggat gtcacgtgcg gatctggaca gtttcgactg gtaacgcggc ggcagtctgg
     3601 cgactctagg atgctggagg tacgccatgc cgctgaatgt caacctgttg cgtcagagct
     3661 tcgaggtggt cgcgaccgcg aaccccaggt tcgtgtcccg gttctacgag atcttgttcg
     3721 agcgccatcc gccggcccgc gcgctgttcc cgcagaacgg gatggcgcgt caggccgaga
     3781 tgctgaccca ggcgctggtc gccgtgctcg atcacctgga ggacgcgccg tggctggtgg
     3841 acacgctggg cgcgctcggc gccaagcacg tcggctacgg cgtgacccgg gagatgtacg
     3901 actgggtcgg gacgtcgttg ctcgcgacgc tcgccgaggt cgcggggccg gcgtggacgc
     3961 ccgagctcca ggcggcgtgg gccgaggcct acggggcgat cgtgtcgctg atgctggcgc
     4021 gcgccgggac gtgatcgatc tcgcaggtgg cggaggctag cgcggactca ccacagcggc
     4081 ggcggcagcg acgtgatcgt ctgcggacgc gccttcgaga tcggcgcgcc ggtcgccggc
     4141 aaggtcggca ggaactgcgg cggccggcgg gcgtgggtcg cctgctcgaa cgcgaacgcc
     4201 agcccgatca gcttgggctc ctcgaaccgg ctcgcgaaga acgagacgcc gaccggcagc
     4261 tccccggaga agcccatcgg caccgagatg ttgggatagc cggcgaccgc ggccggagcc
     4321 gacgagccga acacgaagtt gtcgccctgg ccgagcgtgg tgacccacgc cgggctgttg
     4381 gtcggcgcgg cgatcgcgtc gaggtgcagg tcggccatgg tctgatcgat gctctgccgg
     4441 gccgagccgg tcgccgtcgc gcgctgggtc aggtagtcgg ggtcggtcag gtcgccgctg
     4501 aacgtctgcg acagatcgaa gatctcctgg aggaagaact gcagctcggt atcggcgtgc
     4561 gcgagatcga agtcgatcag gccctggagg tcggcggggt gattgccgcc cacgctggac
     4621 agataggctt tcatgtcgtg cttgaactcg ttgagcagcg cggggaactc gttgttgccc
     4681 acgacgtcct ggagcggcag atcgacgtcg accgtggtcg cgccggcggc ctgcagcgcg
     4741 gcgatgccct gcgcgaacac cgcgtcggtc gccgggtcgc cgccggtgac ggtgcgccac
     4801 acgccgatcc gcttgccgcg cagcgcgttg cggtcgagga accgggtgta gtcacgcgcg
     4861 atgtggccgg cgctggccgc ggtggccggg tcctgggggt cgatgccggt cagcgccgac
     4921 agcatgacgg cggcgtcggt cacgctgcgc gccatcgggc ccgcggtgtc ctgctcggcc
     4981 gagatcggca ccacgccggt ccggctgatc agcccgaggg tcggcttgat cccgacgatg
     5041 ccgttctggc ccgacgggca gacgatcgag ccgtcggtct cggtgccgat cgcgacgacc
     5101 gcgaggttcg ccgcgatccc ggcgcccgag cccgagctcg agccgcacgg gttgcggtcg
     5161 agcacgtagg ggttgttggt ctgcccgccg acgccgctcc acccgctcga cgacaggaac
     5221 gaccggaagt tcgcccattc ggacaggttg gccttggcga ggatcaccgc gccggcgtcg
     5281 cgcagccggc tcacgaggaa cgcgtcgcgg ccgcgcgcgc cgagcagcgc ctccgagccc
     5341 gcggtcgccc gcacgttctc ggtgtcgacg ttgtccttga gcaggaccgg gatgccgtcc
     5401 atcggtccgc ggaggtggtg cgtcgcgcgg cggatgtcgc tgaacgcggc ctcgatcagc
     5461 gcggtcgggc tggtcgcgat cacggcgtgg agctcggggt tgaggtcctc gatccggtcg
     5521 aggtagaacg ccgtcaggag gaccgacgag atccggccgt ggtccatcgc gtcctggagc
     5581 tggggaatcg tggcgcgctc gaggtcgatg ccgcgcagca tcggatcccc gtggcgcgac
     5641 gcgttcgacc cggcggtcgt tgtgccggca ccgagcgcga agcccagcag cagcgtggcc
     5701 gccagggtga cccggtgtct gaacagctga ctgaaaatcc gcattgcgaa cctccgaagt
     5761 ggattttttc aatagggacg ctaggccccc cacatcagcg gttcaagcat tgttttcatg
     5821 acgattgcca atccggacgg agatcggcat ctcgcgcaac tgcctgttcg acgtctgcaa
     5881 cgatgcgagc caccgtcaga cgcgcgtgac gtgcaacaac gccacgcccg cgatccgatc
     5941 tgcgagagag cccacagaag cggtcgaaag cggcgcgcga tcgcgaggcg tcgcacgcgt
     6001 cgcagggcaa tccgccgcgg ccggccggcc ggttcacaag gcgcgccggc cggcgcggga
     6061 cgactagcgg atctcgagct tgccgacgcc gaagtcgacc tgtccgacga agtcatcgcc
     6121 gaactggtcg cgcaccgcgg cgcggaacat cgccgtcggc tgcgcgccgt cgaacaccgt
     6181 gcccggcggg ccgttctggg tcagcttgag cttcaccgag tagaacacat cgccggcgtt
     6241 gacctgggtg tcctggatgg agaccgggtt ggttccgttg gcgttgttgg gggtgcgctt
     6301 gcgcgacacg ccggtgtaca gcacgtgatc ggatccgatc acgccggccg aggcgtccac
     6361 accggtgccg gccggcagcg ccagcccgcg cgtgaacagc ggggtgcccg caccgacccg
     6421 cgtggtgtcg agcggcaggt tcatgccggc ggtgaacgag cccgggccgc cgaagaacga
     6481 cgacaccgtg agccgctcgc tggccaccag gtcgagctgg atggtctgcg ggttcgacgc
     6541 ggcgttcgcc accagccgca ccttcccggt ggtcggcgcg gtgtagacca ggccgtgcac
     6601 ctgggcgacc gcgctgccga gcgcctgcgg gccgctggcg gcggtgccgg tggccgacag
     6661 cgtctgcgtg cccatcgtga cgaacgtgac ggtcgtggtg gagacgccac cctcggagcc
     6721 ggagaacgtg atgctcgccg gcgtcgtcgc cccggtgccc ttgtccgtgg tggcgaagct
     6781 caccgtgccg gcgtagttcg ggatcgcgtt ctcgaacagg tccctgatga cgacgtcgac
     6841 gttcaccggg aagccggagt tggcgttgag cggcagcccg agcaccacct tgggagcgcc
     6901 ggggccgacc gcgacgctgg aggagctggc cgatggcagc gcgagcgcca cgtcgttgat
     6961 ctgcacggca tggtttccgg tcttgatgaa cgtcaggctc accgccagca cgcccgcgct
     7021 gaacgtgccg tccgacggca gcatgtcggt gctgtcggcc gacgtcaggt ggagcttgcc
     7081 gccgtaggcg gtggcgacgt tgccgaacac gtcgagcacc ttgacggtga gcgtcagggc
     7141 ctggccggcg gtcgcgctcc ccggcagcgg gtcgagctgg tacgagtgcg ccgcggcggg
     7201 ctggacggtc agcgacgccg agcccgacgc gttggccttg cccgtgacat cggtcgcggt
     7261 cagcgtgctg aggcccgcgg ccttgagcgt caccatcacc tgcttcacgc cggcctcccc
     7321 cgccatgaac gtgacgtccg cggggagcac ggcgttggcg tcggtgctcg agacgtgcac
     7381 cgtgccgcgg aagcccatgg ccgggttgcc gtcgggtccc agcacggtca gcgtgaccgc
     7441 gaccggagcg ccggcggtcg gcgtcttggt cgccggctgg accttgatcg cggccgccga
     7501 cgtggcgccg accgtgactc gcacggtctg gttggtgacc gccttgccgc tggtcgcggt
     7561 cagctggtac gacgtcgtgt ccgtcggcgc gaccgatacc tggctcttgc cggtgagatc
     7621 gcccatcccg gtgatcgaga ccttggcgtt cgccggctcg accacgaaca ccagcgtggt
     7681 cgactgaccg gactcgatca cgtcgggcga cgcctggaac agcaggatct ggacgtggtc
     7741 gccgttgtcg ccgcacgcgg ccagcgcacc agcgaccaac agggtaagga gcggaaggat
     7801 ccgtctcatg gcgcacctcc gaacttggcg agcagcgcac tcaagtcgtt gtcatccagg
     7861 gttgtggtgg ctcccgtctc gctgaggttc atctcggagt cgtagagcgg gacctcggcc
     7921 ccggcggccg tgacggtcag ccggttggtg aggttgccga ggtcgcggaa gtcgatgatg
     7981 ccgttcttgt cgaagtcgcc gagcccgagg gccccgttgg ctccgtcgga gcgcgggtag
     8041 atctcccaga ccccgcggcc gtacatcgcg gcccggatca gcgagccgtt gagcgacagg
     8101 aacaggtcct ggacgcggac catcggcagg ttgctgccgt agcgcgccca ggtcttgccc
     8161 tggtcggtcg agcgatacag gccgagatcc gtgccggccc acagcgtctg atcggtcacg
     8221 tcggacggat cgaaccggat cacgtagacc cggacgttcg gcaggtcgaa gccggtgccg
     8281 ttgccgtgca gcggcgtcca ggtggtgccg ccgtcggtgg tcaggaacac gtggccggtc
     8341 gcggcggaga tcggcgtcgg cgggttggtc accggcgccg tgacgaagtc gccgatgctc
     8401 gacaccacgt aggtcttcgt gatgtcggtg ccgccgaggt gggtcgggtc tttcgggaac
     8461 gtgatcgagg cggtgttctg cacctgctcc gggccgatct gcgggatggt gttcgcggtg
     8521 accacggaca cgacgccgtc ggcgcggccc ggatcgacga ccaccgcgta gcagccaccg
     8581 gcgagcggca ggcccaagat ccgcgacgca acgccgttga ccgtgtacgt gaacggcgag
     8641 gcctgcgggc caccggcgcg gatcgtgcgt gcggcggtcg atccgcagcc gacgtagcca
     8701 cccgggttgg gcggcgccgg gctggtgacc agccgcacga tcgacacggt gtcgcccgcg
     8761 cccggggtca gcttccacag gttgaagttc gagttgctga ccacgctgcc ggcggcgtcg
     8821 tacagcggcg cgtagcgcac cgcgaagccg ttgttggtgt caccgggcgg cagcgtcggc
     8881 gtcacccgga agtacgaccg gatctcggag tcgttgacca cgtgggtcgc gcgcgtgcac
     8941 tcgacgccgg cgccggcccg gcacgcgatg cggccgccgt tggcgacgcc ccaggtcgtg
     9001 acgttgccgc cggtcgcgtc gttcgacacc gcggcgccga agccgtcacc gccctggatc
     9061 tggttccacg ccttggggaa gtttgacagg tagaactcgc tctggctgac ccggatgcgc
     9121 gtgccgttgt cctgcaggcc ggtcagcacg tacttggcgg tgccgaacac cggatccccc
     9181 gagccaaccg agtagggcag gagggtgtcg agcccgatgt tggcgtcgaa gaagtgcacg
     9241 tccgagccgc gcgccgccga gaacaggtcg aacgaggcga agatgccgcc gtcgctgctg
     9301 gccagcgcga tcggctggcc gttgacccgg atgatgtggc tgttgtgcca gtcggcgtgc
     9361 acgtagggca gcgagccctc gtcgccgccg aacgccagcc agtccgacgc gatctgccag
     9421 gtggtgccgc cgtcgaccgt gcgcgcgccg cacaggttgc cgccgatcat cacgttgttg
     9481 gggttgcccg gatcgaccgc gaccgcgagg tcgtactggc tctggccgtg gccgatgtcg
     9541 aggttcaggc agtcgccgcc ggtggcgccg ggcgtcgggt tggtcggcga ggtgagcgcg
     9601 ccctgcgcga ccgagaacca cgaggtgccg ccgttggtgc tcttgagcac ggcgatggta
     9661 tgggcgccat tctgatcccc ggcgagcgcg tagatgatgg cgctgtccga gttcggcgag
     9721 cccgggatcg cggtcaggtt gatccgcccc atctcgctcg gcaccggcgc ggggggagtg
     9781 ccggcgagct cgaacgtcgc cgggaacagc ccggcgaccc gcgccgagct ccaggtggcg
     9841 ccgccgtcgt tgctggtcca gatgtcaccc aggttgccct ggccggtcgg catcccgggg
     9901 cactgggtga cgaagaaccc ggtcgtcggc tggttgaacg acggcgggta cgtgccgggg
     9961 catgcgtagt tgccgctgac caggaacgtc gacaggccgg tcgccgccga cacgccggtg
    10021 tagacgacgc tgaatgtgcc ctcgaggtcg ttgccgtacg cggtggtgtt gggcaggtcg
    10081 atcggcgcgt agtgcgcgcc gccgtcggtc gagcggaaca gcccgacgtc gctcgcgacg
    10141 aggatgttgt tcgggttgtt cggatcgatc cgcacggtgc gggtgttgac cgcgtggatc
    10201 gccgtgctgt tgagcgtggt ggtgagcagg tggggcgcgc tccaggtcgc gccgccgtcg
    10261 gtggaggtca ccatcacgcc gcccgggttg ccttcccagt aatcgccgag gccggcgtgg
    10321 atgatgagcg agctggtcgg gtcgatgtcg aacgacccga tgaacatcgt gccgagcgtg
    10381 ttggtcagcg gctgccagac cggctgggtc accgtgatgt ccggcgtctt ccagatgccg
    10441 ccgccgatcg cgccgatgta cacggtcgcc gggttctgcg ggtcggtgcg gatcgtggcg
    10501 acgcggccgg agtcgaggcc gtcgtagtag ctgccgttcc actccgacag cgcgctctgc
    10561 ggtccgagct ggacccactg cagcggggtc gtcggtgtcg gcgacggtcc gccggcatcg
    10621 gtgatctgcc gggccgcggt gctgtagctg gcctcgcgct gctgctgcac ggcggtctcg
    10681 tgcatcaggt tgacgatgcc ggcctgcgag gccgggccgt tggtcagccg cagccacagc
    10741 gagcgaacca gcgcatcgtc cgccacgacg tcgctggtat cctcgacgta gttgatcggc
    10801 agcgggccga tgccgtcgtc gccgcgggcg atcgcgtcct tcagggcagc aatggtcaca
    10861 tctcgctggt tctgtaattg ctgttgctgg gatggcgagt tgaccgcgtg cgccacgatc
    10921 acagtcgtcg tgagccccac ggtcacgacc acgctcccgt agatggcttt cttcaatctc
    10981 cgagcattca tgttcggcac cccccggtgt gttcaggagt gaaaggcgaa gaattttgcg
    11041 tgggtcaggt tacgtctgcg cttcagtggg cttctaggag tgaagctggt tctacggtga
    11101 aatctcatct aagacagatt ttatagtaat taaaactaca tttaaatgct attttcaata
    11161 gaatcaaact catatgtgat cgaagaacgc ccttggccaa ggccgagccg ctccctgcct
    11221 gctagatttt cttaattcga gtaagatcct gttagtggtc tgtaatacga cgtgacacgc
    11281 cgatgtaagc accgtggcgg atgggcacat gcaccccggt gcgcgccaac ccaggggatc
    11341 gcgcggtcgc gcacgggctg cgcggaccgg gcatctgttc ctgatacgaa acagatagcg
    11401 gcctgtcgac ggcgggtccg ctgcgctacc gcgaggcgga ccgccggggc cgcgggatca
    11461 cgacggactc cgggcgagtc gcggcgcacc gcttgcgccg cgccgggcga tcgcggaggg
    11521 tagtgcgtcc aggtcatgcc cctccgcgtc gtgatccggc tgatggtcct cgagtcgctg
    11581 cgcgcgctcg cccgcaacaa ggtgcgcagc ggcctggcga tgctcgggat cgcggtcgcc
    11641 gtcgcgatcg tcatcacggt gatcgcgctc ggccgcgcca gcatcgcggc gtcggaggcc
    11701 gagctcgaca agctcggcga caacctggtc tgggtcgagg ccggctcgcg caacgccgcc
    11761 ggggtgcgca ccggcagcca cggcatgacc acgctgaccg cgcgcgacgc cgacgcgatc
    11821 cgcagcgaga tcccgctgat caaggacgtg tcggagaacg tcgatggcgg gttccaggtc
    11881 gtcgcccgcg accacaactg gcagacccgg tggcgcggcg tcgcgccgag ctaccaggcg
    11941 atcaagcgct gggacatcgc gcgcggccgg ttcttcgacg acgatcagac cgagcacgcc
    12001 gcgcgcgtca tcgtgatcgg cgagaccgtg cgccgcgagc tgttcccgga cagcgagccg
    12061 atcggcgaga tcgtccggat ccgcggcctg ggcttcgagg tcatcggcgt gctcgccccc
    12121 aagggccagt cggccaccgg gcaggaccag gacgacaccg tgatgatgcc gtggaccacg
    12181 gcgatgaagc agatcgtcgg caaggaccag acctggctcg acgacatcct gtgctcggcc
    12241 cgcgcgaccg acgcgatcgc gccggcg
//
LOCUS       DASVOR010000503         7906 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_180055,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000503 DASVOR010000000
VERSION     DASVOR010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7906
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180055"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VF516_10050"
     CDS             <1..402
                     /locus_tag="VF516_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108058.1"
                     /translation="RSGALAACDPPVAALLERGVWSQRRRFSRVRETRFFVWSAWPSP
                     VYVGITPQSSWVSVLARQKQATDTAPSFTGDYFLFSYNTDERGWAYKERPPGAGVLRD
                     LLGAMGRRGLLTAPIEPLLAPEEPEDDRGDA"
     gene            1251..1613
                     /locus_tag="VF516_10055"
     CDS             1251..1613
                     /locus_tag="VF516_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108059.1"
                     /translation="MTKLNARFNPLSLRFAATYRPDRQGNPSSSNQRWVVFRGAVDQE
                     LDPLEFYRIVGREDLAASYERRQVLMFGSYVVATFGIVVGMELQLTPNPTSARVRRCR
                     QPSVRSASSIMSPRACRR"
     gene            1601..1843
                     /locus_tag="VF516_10060"
     CDS             1601..1843
                     /locus_tag="VF516_10060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108060.1"
                     /translation="MPALIGAGVGLGAAMVGTYFYRHLQPIDESEARSLAALYNHQLI
                     RQLGRPGVARRPALRDLTLRPYAARDDAGLVLGARF"
     gene            2007..2588
                     /locus_tag="VF516_10065"
     CDS             2007..2588
                     /locus_tag="VF516_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108061.1"
                     /translation="MREMERRREVILTAMNNLGSRTRTFVIAVRDRIELGGYRSSCVG
                     SCDWDAHNPTGQPGLGLEIDVADRNVINRAMARLLSVEVIAHPPEWLSQGIVEYFNPR
                     GRRGSSPGVRDVGQLGPGVPAGRTGPDPPLTQVAGRPPAGTPGRDPARRSPRSGSDDE
                     GRSTEWPRRQRCGASTRPLRSTPSWSTRPGRTM"
     gene            3015..4169
                     /locus_tag="VF516_10070"
     CDS             3015..4169
                     /locus_tag="VF516_10070"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8108062.1"
                     /translation="MRLGAVHRARPVESPESPRNPGVGATPGMAHRLHRRRFMQLHPA
                     SYYVHELKPNMPVEIFEPSPLRLIWLPIHLAVISSGTLVIAGRWLPWPLIPVISLVIG
                     MSFGGLMFLGHEVLHGAIVRGRWNWLRPIVGWICFAPFALSQQLWVVWHNRVHHANTN
                     KLDADPDMYPSLARYQTSRVTRWAIDNFALGGRRKRGVLSLLFGFFVQSKDMLRSGRS
                     RLGMSHREYRRAIVETVLAIALWIALAALVGLVAFVFAFVIPLLIADVIVMGFILTNH
                     CLSPATDINDPLINSLSVTAPRWIEWVTLEFGYHVEHHIFPSVCGRHARRVRQGLRER
                     WPERYQSMPVMASLRALHRTGRVYKDANTLVDPRSGGEWPTLMPRVEHQA"
     gene            complement(4387..5457)
                     /locus_tag="VF516_10075"
     CDS             complement(4387..5457)
                     /locus_tag="VF516_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8108063.1"
                     /translation="MDGLDPAIRSHRVEGVNGLSMHCLEAGEPGRPCVLLLHGFPELA
                     YSWRKVIAPIAAAGFHVVAPDQRGYGETTGWDGRYEADLAPFGMLNLVQDCVSLLSRL
                     KIAEAHVVGHDFGSPVAAYAALTRPDVFSSVVLMSAPFGGAPIGGRATGVHDDLAKMG
                     RKHYQWYYSTREADENMRRCPQGVHAFLRAYYHYKSADWPGNKPFPLRGWIASELTKM
                     PTYYIMELGEGMAETVAPYMPAREAPWLTDEELRIYASTFERNGFQGGLQWYRCATNP
                     RHAAELTAAAAGKTIDVPSMYIAGASDWGVHQKPGEFERMQGGTCSDFRGAHLIPGAG
                     HWVQQEQPEKTAALLVDFFSRA"
     gene            complement(5539..7098)
                     /locus_tag="VF516_10080"
     CDS             complement(5539..7098)
                     /locus_tag="VF516_10080"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX8108064.1"
                     /translation="MLRGIARVPVALSTAAGCTAAPSSEAPGAEAVGTASSDQALSSA
                     FATTMAGLAPVGWWRLGETTGTAADSSGNGHSGTYHNFLSTQRGIAGAIAGDSDGAIQ
                     VTPVASPSCANSPFVDVADNDGFSLTRAQDSFDRSVALGSTWGTADHGGGWTAEVTTG
                     SFYSVDGSHALIAETTAGTWQIGLPVSRKDADIQVRASWSQAAVGGALTPLAIVARRI
                     DNANAYRAEIRENPGGQLDLRIVKLVAGTSTVLQTATAIGTYSLHGAGTADDDWWYVR
                     FQLEGTGLRARAWKLGTAEPTTWTVTATDSSLTAAGNVSIRSANSGTTSLPTVAFEGF
                     RVQSVGMTVHAFMKPTALDFNGGNYVHWLGKGEDPQMEWGFRFYPSSNPDRPKRVSGY
                     IWNSTACCGFSTNEGAGAYFQDPALAAGTWYEVVVVYDPGDDLDTQAGVTIYQDGQCK
                     SGTQCGGTPSPGVLYSNASFVVYPLNGTSPLRIGTRDCGSFFTGGLDEVAVFDRRLTA
                     AEISELHTQSQ"
     gene            complement(7363..>7906)
                     /locus_tag="VF516_10085"
     CDS             complement(7363..>7906)
                     /locus_tag="VF516_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108065.1"
                     /translation="MWEASDVLFVAINLPGGSNNDTDVWYGAPTASPAQLAETADRTA
                     ADLRWLDAAFARAREAETRAVVIQLQADMWDTEKGPAHQAAYEPIVASIASHTLAFGR
                     PVLLVNGDSHVFRSDNPLAASDPLNFMHPGYDVPNFHRIIVHGSTFPLEWLRLTIDRH
                     GAPGPDSFGPFHWERVIPQL"
BASE COUNT         1193 a         2777 c         2688 g         1248 t
ORIGIN      
        1 cgctccggtg cacttgctgc ctgcgatcct cctgtcgcag cactgctcga gcgcggcgtg
       61 tggagccaga ggcgccgctt ctctcgggtg cgcgagacca ggttcttcgt gtggtccgct
      121 tggccgagcc cagtctacgt cggcatcacg ccgcagagct catgggtgtc cgttctcgca
      181 cgccaaaagc aggccacgga caccgcgccg tcgttcaccg gtgactactt cctgttctcc
      241 tacaacaccg acgagcgcgg gtgggcgtac aaggagcgcc caccaggcgc cggcgtgctg
      301 cgcgatctcc tgggtgcgat gggccggcgt gggctcctca ccgcgccgat cgagccgctc
      361 ctggccccgg aggagccaga ggacgacagg ggcgacgcct gaagcccccc cgggggcgcg
      421 ccggccgggc acggtccgag tcgcctcgcc gccgccgcgg tcggtcgtgc gaggccgaga
      481 gcgcggctcc gacgagaacg caacgcagtc ggcccaccgg catcaccagg tggcatcgcc
      541 agccaacccg ccctggtgcc gatcttgacc cggccgacgg ctcatggccg gcgggtccgg
      601 ggcgccgtct accgcgcgtc taccgcggcg ccagcttccc cgacgaagct cgcacgctat
      661 tcacgctgca ggcgctgaca aaggcgaggg atgctcagga cgtacgcgtc gagtccacga
      721 agctcaagga ctacgacatc gaaagctaga gcgaaacacg cgaactgacg agcaccagga
      781 tccgctatct cccgcgcatc taccgtgacg gcgccgcggc cggccggcgt gcattggctt
      841 cgcgcggaag caccattgcg cgcggtgctg cgctctggca caagtgtacg tgtgtggcgc
      901 cgcggactga tctgggccgt cctggtcggg tctcagactg ctctggcgca ggcgcctccc
      961 gcggagccag ccgccgagcc cccggtagcg ccgagcgagc cgccgagcgc ggcgttcttc
     1021 gagcagcacg tcgcggagca actcgccacc cagggcgtgg tgatgtcgca gcgcagctcg
     1081 gcctccggat cgagtacatc gaggaccact ggaccgtatc tctgatcgat ctcgccaccg
     1141 ctcaacccgc ggcagcgctc gagatccgcc agctgccagc ggaacgcgat gcggcgatcc
     1201 agatcctggc ccgcgcggcg gcaaatcttg cccagcacct cgcgagcgag gtgaccaagc
     1261 tcaacgcccg gttcaacccg ctatcgctgc gcttcgcggc gacctatcgg cccgatcgcc
     1321 agggcaaccc gtcgagctcg aaccagcgct gggtcgtttt ccggggcgct gtcgatcagg
     1381 aactcgaccc gctggagttc tatcgcatcg tggggcgtga ggatctcgcc gcgtcgtacg
     1441 agcgccggca ggtgctcatg ttcggcagct acgtcgtggc caccttcggc atcgtggtcg
     1501 ggatggagct gcagctcacc ccaaacccga cttcagcgcg tgtgcgccgc tgccgccaac
     1561 ccagcgtccg cagtgccagc tcgatcatga gccctcgagc ttgccggcgc tgatcggcgc
     1621 gggagtgggc ctcggcgcgg caatggtcgg tacctacttc tatcgccatc tgcaaccgat
     1681 cgacgagagc gaggcgagat ccctggccgc gctctacaac caccaactga ttcgccagct
     1741 cgggcggccg ggcgtagccc gacggccggc gctccgcgac ctgacgctga ggccatacgc
     1801 cgcccgggac gatgccggcc tggtgctcgg cgcgaggttc tgaccggatg tgggatgcgc
     1861 gatgggcagt ggcaatgctg gtggcggcag cgtgtggtgg accgctgatc cagctgccga
     1921 gtcatggcgg tccggcctgg ctcgaagtgc agagtgagca cttcacgctg tggacggatg
     1981 gatcggcgga gcgcggccac gagctcgtgc gcgagatgga gcggcgccgc gaggtcattc
     2041 tgaccgcgat gaacaacctg ggatccagaa cgcgaacctt cgtgatcgcg gtgcgcgatc
     2101 gcatcgagct cggtgggtac cgatcctcct gcgtcgggtc atgcgactgg gacgcgcaca
     2161 acccgaccgg gcagcccggc ctcgggctgg agatcgatgt tgcagatcgc aatgtcatca
     2221 atcgcgcgat ggcgcgcctg ctcagtgtcg aggtcatcgc acacccgccg gagtggctca
     2281 gccagggcat cgtggagtac ttcaaccccc gagggcgacg aggctcgtcg ccgggcgtgc
     2341 gcgatgtcgg gcaactcggc cccggagtgc ccgcgggccg cacgggcccc gaccctcccc
     2401 tgacgcaggt ggctgggcgt cccccagccg gcacgcccgg ccgcgatccg gcccgtcgct
     2461 caccacgatc tgggagcgac gacgaaggcc gatcgacgga gtggccgcgg cggcagcgct
     2521 gcggcgcctc gacgcgacca ttgcgaagta ccccgagttg gtcgacccga ccgggccgga
     2581 caatgtgagc gcctggatcg aaacactcag gagcatcgaa cgaaggaatc gacatgaccc
     2641 tggtatcgat cttgacccgg ccgccggtgc acgccggcgt tggtcccgcc gccgtctacc
     2701 gcgcgtctac cgcggcgcgg tcgtcctcca cgaacggcct ggcccaccga tcgccggttg
     2761 ccgagtgcgg tgcgcgtcga gccgcatcca ctcgagcaca cggccggtcg ccaccgccgc
     2821 cggcatcccg ccgagcgagc cgctgcgcgc ggtctccggc gtgtgcaact ggggtgggat
     2881 ctgatgcctg gcgcggcgga tcgccggctc gccgcgacct cctcgaccca gcgcgggcag
     2941 ctcgcgatcg cgccgagctt cctcgattgc gcattgcttg cgacccgcgt cgatcgcgat
     3001 gcaaggcgtg ctcgatgcgg ctcggcgcgg tgcatcgggc gaggccggtc gagtccccgg
     3061 aatcgccgcg aaatcccggt gttggcgcca caccggggat ggcccacagg ctgcatcgac
     3121 ggcgcttcat gcagctgcat ccggcatcct actacgtcca cgagctgaag ccgaacatgc
     3181 cggtcgagat cttcgagcct tcgccgttgc ggttgatctg gctgccgatc cacctcgcag
     3241 tgatctcatc cgggacgctg gtgatcgccg gccgctggtt gccgtggccg ctgattcccg
     3301 tgatctcgct cgtgatcggg atgagcttcg gagggctgat gttcctcggc cacgaggtgc
     3361 tgcacggggc gatcgtgcga ggccggtgga actggctgcg cccgatcgtc ggctggatct
     3421 gcttcgcacc gttcgcgctg tcgcaacagc tctgggtcgt ctggcacaac cgtgtccatc
     3481 acgcgaacac caacaagctg gacgccgacc ctgacatgta cccatcgctt gcgcggtatc
     3541 agaccagccg ggtcacgcgc tgggcgatcg acaacttcgc gctgggcggc cggcgcaagc
     3601 ggggtgtgct cagcctgcta ttcggctttt tcgtgcagag caaggacatg ctccgcagcg
     3661 ggcgcagccg cctcggcatg tcgcatcgcg agtatcgccg cgcgatcgtc gagaccgtcc
     3721 tcgcaatcgc gctgtggatc gccctggccg cgctcgtcgg ccttgtcgcc ttcgtgtttg
     3781 cgttcgtcat tccactcctg atcgctgacg tgatcgtcat gggattcatc ctcaccaacc
     3841 actgcctcag ccccgccacg gacatcaacg atccgctgat caactcgctg tcggtgaccg
     3901 cgcctcgctg gatcgagtgg gtgacgctcg aattcggtta tcacgtcgag caccacatct
     3961 tcccgtcggt gtgcggacgc cacgcgcggc gcgtacgcca gggactccgc gaacggtggc
     4021 cggagcgcta tcagtcgatg ccggtcatgg ccagcctgcg cgcgctccac cgcacggggc
     4081 gggtctacaa ggatgcgaac acgctcgtcg atccgcgttc gggcggcgag tggcccacct
     4141 tgatgccgcg cgtggaacac caggcttgaa agcacgacag cgatcagcgg tggacgcgcc
     4201 ccgcgtccgc gcagccatga ccgggcgacg gctcgcggcc gtcgtgggtc acttcgacca
     4261 gccgcacaga tcgccgatcg cctcgacgac gcggtgcttg acacccggcg cttcgctgct
     4321 atcgggatct cgtgggcgcg catccagtcg tactgcgcgc gcgcggtctt gcggtcggcc
     4381 gcgagttcaa gctcggctga aaaagtcgac gagcaaggcg gccgtcttct ccggctgttc
     4441 ctgctgcacc cagtgtccgg cgccgggaat caggtgcgcg ccgcggaagt cgctgcacgt
     4501 cccgccctgc atgcgctcga actcgccggg cttctggtgc acgccccagt cgctcgcgcc
     4561 ggcgatgtac atcgacggaa cgtcgatcgt ctttccggcg gccgcggcgg tgagctcggc
     4621 ggcgtgccgc gggttcgtgg cgcagcggta ccactgcagc ccgccctgga agccgttgcg
     4681 ctcgaatgtg ctcgcgtaaa tgcgcagctc ctcgtccgtg agccacggcg cctctcgcgc
     4741 cggcatgtac ggcgccacgg tctcggccat cccctcgccg agctccatga tgtagtacgt
     4801 cggcatcttc gtcagctcgg acgcgatcca gccccgcagc ggaaacggct tgttacccgg
     4861 ccagtcggcg ctcttgtagt gatagtaggc gcgcaggaac gcgtgcacgc cctgcgggca
     4921 gcggcgcatg ttctcgtccg cctcgcgcgt cgagtagtac cactggtaat gcttgcgccc
     4981 catcttcgcc agatcatcgt gcacgccggt cgcgcgcccg ccgatcggcg cgccgccgaa
     5041 cggcgcgctc atcagcacca ccgagctgaa cacatccggc cgcgtgagcg ccgcgtacgc
     5101 ggccaccggc gagccgaaat cgtggccgac cacatgtgcc tcggcgatct tcagccgcga
     5161 gagcagtgac acgcagtcct gcacgagatt cagcatgccg aacggcgcga gatcggcctc
     5221 gtagcgtccg tcccatcccg tcgtctcgcc gtacccgcgc tgatccggcg cgacgacgtg
     5281 gaaacccgcc gccgcgatcg gcgcaatcac cttgcgccag ctataggcga gctccggaaa
     5341 cccgtgcagc agcagcacgc acggccgccc cggctcgccc gcttcgaggc aatgcatcga
     5401 caggccgttg acgccttcga cgcggtggga gcggattgcg ggatcgagac cgtccatccg
     5461 ccgattctag cgccggcggc acgcacatcc ctcacgggac acggtagaag tgggcgtccg
     5521 agaatcccga gcccggagct actgcgactg ggtgtggagc tccgagatct ccgcggccgt
     5581 gagccgccga tcgaacaccg ccacctcgtc gagcccgccg gtgaagaacg agccgcagtc
     5641 gcgggtaccg atccgcagcg gcgacgtgcc attcagcgga tagaccacga agctcgcgtt
     5701 cgagtacagc acgcccggcg acggcgttcc tccgcactgc gtcccgctct tgcactggcc
     5761 atcctggtag atcgtgacgc cggcctgcgt atcgagatca tcgcccggat cgtagacgac
     5821 caccacctcg taccaggtac cggcggccag cgccggatcc tggaagtacg cgcccgcgcc
     5881 ctcgttcgtg gagaacccgc agcacgccgt cgagttccag atgtaccccg agacccgctt
     5941 gggccgatcc ggattcgacg atgggtagaa ccggaacccc cactccatct ggggatcttc
     6001 gcccttgccg agccagtgga cgtagtttcc gccgttgaag tcgagcgcgg tcggtttcat
     6061 gaacgcgtgc acggtcatgc ccaccgactg gacgcggaag ccctcgaacg cgacggtcgg
     6121 gagcgacgtc gtcccggagt tcgcggaccg gatgctgacg ttgcccgccg cggtgagtga
     6181 cgagtcggtg gcggtgaccg tccaggtggt cggctcggcc gtgccgagct tccaggcccg
     6241 cgcccgcagg cccgtgcctt cgagctggaa ccgcacgtac caccagtcgt cgtcggcggt
     6301 gcccgcgccg tgcaggctgt aggtgccgat cgcggtcgcg gtctgcagga cggtcgaggt
     6361 gccggcgacg agcttgacga tccggagatc gagctggccg cccgggttct cccggatctc
     6421 ggcgcgatag gcgttcgcgt tgtcgatgcg ccgggccacg atcgcgagcg gcgtcagcgc
     6481 gccacccacc gcggcctggc tccacgacgc gcgaacctgg atatcggcgt ccttgcggct
     6541 cacgggcagg ccgatctgcc acgtccccgc ggtggtctcc gcgatcagcg cgtggctgcc
     6601 gtcgacggag tagaacgatc cggtggtcac ctcggcggtc cagccgccgc cgtgatcggc
     6661 cgtgccccac gtcgagccca gggccacgct gcggtcaaag ctgtcctggg cccgggtcag
     6721 cgagaagccg tcgttgtcgg ccacgtcgac gaacgggctg ttggcgcagc tcggcgaggc
     6781 caccggggtg acctggatgg ccccgtcgga gtcgcccgcg atcgcgccgg cgatcccacg
     6841 ctgagtggac aggaagttgt gataggtccc gctgtggccg ttaccgctcg agtccgcggc
     6901 agttccggtg gtctcgccga gccgccacca cccgaccggc gcgaggcccg ccatggtggt
     6961 cgcgaacgca ctcgacagtg cctgatcgct cgatgcggtg ccgaccgctt ccgcacccgg
     7021 cgcctcactc gacggcgccg ccgtgcagcc ggccgcggtg gacagcgcga ccggcacgcg
     7081 ggcgatgccg cgcagcacgg cggtgagccg gcggcgcgcc gcggccggtg cagatacggc
     7141 atgatcagag cggtccaggg gtgagccgtg gcgggacact cgatgcatgc gagagtacca
     7201 cgcaattacc atgcgggatc ctgcacgcag cggccgggcc tggaccgccc gggcatgcgg
     7261 caacctcgcc cggtgggctc gatgtccacg cgccccatcg ccctcggagc gacccggacg
     7321 cagcaccgca ccgctcgcac cgtctcgtat caccgatgcc ggctacagct gtgggatgac
     7381 gcgttcccag tggaacgggc cgaacgagtc gggaccgggc gctccgtgtc ggtcgatcgt
     7441 gagccgcagc cactccagcg ggaacgtgct gccgtggacg atgatgcgat ggaagttcgg
     7501 cacatcgtag cccgggtgca tgaagttgag cggatcgctc gcggcgagcg ggttgtcgga
     7561 gcggaacacg tgcgagtcgc cgttgacgag cagcacggga cggccgaacg cgagcgtgtg
     7621 cgaggcgatg ctcgcgacga tcggctcgta ggccgcctgg tgcgcgggtc ccttctcggt
     7681 gtcccacatg tccgcctgca gctggatcac gaccgcccgc gtttccgctt cgcgtgcgcg
     7741 cgcgaacgcg gcgtcgagcc agcgcaggtc ggcggcggtg cgatccgcgg tctcggcgag
     7801 ctgtgccggc gatgcggtcg gggcgccgta ccagacgtcg gtgtcgttgt tcgatccgcc
     7861 cgggaggttg atggccacga acaggacatc cgatgcttcc cacatc
//
LOCUS       DASVOR010000504         8229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_501440,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000504 DASVOR010000000
VERSION     DASVOR010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501440"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VF516_10090"
     CDS             complement(<1..379)
                     /locus_tag="VF516_10090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108066.1"
                     /translation="MPSREPELYKDKIEVSLDGRQIFYLFFGGAVIVGMVFVLGVLVG
                     RRVEARGHLDRTDRADPPASTDPLAALDRLERSDHLSFHGALTGNETPTDVEKAIGEL
                     ERRRAANRGDGPDRAAKAAEAHAA"
     gene            complement(434..4216)
                     /locus_tag="VF516_10095"
     CDS             complement(434..4216)
                     /locus_tag="VF516_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108067.1"
                     /translation="MTRGRQLAASAAVASVAALWGACGNTTNTGVIELNLDRPVDVSF
                     ACYGKMRQTNGRPGPGMLPDPIIKTAMPTEVCQALSPPLSTDPVVPVVGQETLGTKDN
                     PAPDNPNPVWYGFILQSASGTVALTTWPVTPADSMFASGSLPQQISDTPTSKFQVLDA
                     DPLTPGKNAISIGEDPVAIITDKTGCFEVTANAGSCDLSELDITSTLANIVDHSTVRP
                     RIDRVTVTGPDGTPILAKPSAMIVEPFLDVSPGTDKVGNACPKAATSQVYIAYPSCHL
                     VAEVRLAAGADGKATAQVIGGIYFDPSDPSGTASVLTDQTLPAKIQSCPTECAPPRGM
                     PVVTAKDGVRPVALDHKLDTRTDRDVFGTNGVKPDITSKLAIGADIRGAPLAAPLTVV
                     DLDLTTFAPRSGSVLQIPLEANRGPVGISALALSPQIGMGSNTGGGKVNDEADDQAQY
                     VYAVATDGTVRVADVLKLKRECDTQIDGRFVRSTSATTLECPPLGQPNLPRRPGAQGP
                     GIELPGGDVANSVTIVKGLAAPPPVIGQDGKPTANLVGAQPTLLLGYFAFITASSGTV
                     FVANVNDDYVSHNITPTKDHALATSPVLVMPHQLRDAVSDREDPPTDCTTTDGKGGAF
                     AGGPRLTAPPVQQAVAATLFTDLNLQLPRLQQVQCDVNSDVAVTPLDFGAPEIRSSAS
                     DGPAPSRDLAYPDLKSVVDLKNWTPQEGVALENWKLTYEGTLELDSTLNSINGPSLHF
                     GQTFVDGTGMFIADASRPFCSMGVEPGDIVEFQGCNPANIDTDCPSGYTCYVHPLSTV
                     GVGACFLKSEAPRLQDACRDFLSSIRRYTVGTDASGGTAPGKLVLWQRKHELSTTPVD
                     GCVSDDQCDDLARAAVVLNQGADPFAEAAPGTPVTQRPTHWSCMADPLRKPINTDPAR
                     NKRCVQVCDLMPPDPNKPHPVCANGMICAPDPTSVTGGVCMEGVEPPQACLNGPQRYV
                     VRAGEAFTVVGDHSGFVHPIIVSPDGSRCMRDPNLAKDPLSIGRIPLTAPPCGPPPTD
                     PLGVLNYPLTGRLDSATFEPNPCSLSVPQFETTPSYPSSCLDGHPLACPGPDGKLPLP
                     DQQQPPPCSDGQGPLCSDNQPPPRYGTCRGPAQPAKPGQRANFPAIKFRNRSLTLTIV
                     DPYREARQTCLGRDPVDSQTQMPIPTNIPLVVPGYQVTFAVKAGFTPLTVALVGQVSP
                     VKVVRGPSESIWVIDDGDFLATTFVDVSTRGRVFRIESVPPATTDGSTTVIVNTLQ"
     gene            complement(4213..5796)
                     /locus_tag="VF516_10100"
     CDS             complement(4213..5796)
                     /locus_tag="VF516_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108068.1"
                     /translation="MRMATRIAAPARTARSAWRGPAWALVCGAAIGCTASGSDIELHR
                     DELAFPTGVKVSPDGKALFVVNANSELRFDSGSLDVIALDRVQRVISEWTTYLTQPSN
                     TPDPCNTFAQMTLPPKVPDPGGGNALDCKCDPDNAATLGCEESYFINTDAGVKLGNFA
                     TDLSIQDFSAGATTHLRVFVPSRGDPSIAWADFDGTALHCTAGSNSYQLCDDAHRLTS
                     LNNDPNLEVLPNEPFAVFADVVHPVDAMGKPLPLDHTVAPQHGFAMVTHLASGAVTLI
                     DAPADGPVQIADILQGVFGDPSTAARGATSVAGLPMQIPASPAPGAGPELAPETLYVA
                     SDTDNRVQQFSVGMRAGAADYLLPGRFFVLSAVGETSGSSVDSRGLRFSADGTRLYLV
                     NRTPPSLQVYDTSIGPDGTPNNRLLGSSDLCREGSAAAVAGAGTDERVYVTCFDTGQV
                     YVVNPFGQSQVEDIITVGRGPYAADVLSLAASPTLPPPTKFLFVSNFLEDTIAVIDID
                     PSSPTRNRVVLRIGQPRPQ"
     gene            complement(5897..>8229)
                     /gene="ppdK"
                     /locus_tag="VF516_10105"
     CDS             complement(5897..>8229)
                     /gene="ppdK"
                     /locus_tag="VF516_10105"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEX8108069.1"
                     /translation="NLGLNDTTVAALAQRSGNPRFAWDCYRRFVAMYGEVVMGVVAPS
                     EQDEPPFDRILDEVKHAHKAARDQDLSEAALRELVTRFKAEVKQITGQTFPDDVTTQL
                     WGAIGAVFRSWNNPRADYYRKLHGIPSTMGTAVNVQAMVFGNLGDDCATGVAFTRNPA
                     TGTAELYGEFLTNAQGEDVVAGIRTPEPISELAKKLPRAHAELVRVAKLLEDHFRDMQ
                     DLEFTIQSGELFMLQTRSGKRTGKAMVKVAVDMVGEGKLTPRDAVLRIDPAKLDEVLH
                     PTIDPKARPPAIAKGLPASPGAAIGRVVFTANAAKDWAERGETVLLVRTDTSPEDIHG
                     MKAAAGILTSRGGMTSHAAVVARGMGKCCVTSCTSLRVEAAKRTASFVGGGQERKIKE
                     GDTLTLDGSTGEVFLGSAPLVPAELGNELGTLMTWVDSFRRLQVRTNADTPTDARTAR
                     SFGAEGIGLCRTEHMFFQPERILAVREMILAGDEAGRRAALAKILPMQRGDFAELFRV
                     MDGLPVTIRLLDPPLHEFLPHGQAEIEEVAAALGKPAGQVAAKVAELTEANPMLGHRG
                     CRLAITFPEIYEIQARAIGEAAAEVAAAGGAVHPEIMIPLVMHPEELHRLRALVGKTF
                     DAALGGASIPYTIGTMIELPRACVVADQIAAHADFFSFGTNDLTQTTYGLSRDDSGRF
                     LPAYLDAGVLANDPFAVLDPEGVGALIELAVRGGRSVKPDLKVGICGEHGGDPTSVGF
                     CHRAGLNYVSCSPFRVPIARVAAARAALSDAPSGKK"
BASE COUNT         1130 a         2751 c         3070 g         1278 t
ORIGIN      
        1 cagccgcgtg cgcctcggcg gccttcgccg cccggtccgg gccatcgccg cggttcgccg
       61 cgcggcgcct ctcgagctcg ccgatcgcct tctcgacgtc ggtcggcgtc tcgttgcccg
      121 tcagcgcacc gtgaaacgac aggtgatcgg agcgctccag gcgatcgagc gccgccagcg
      181 ggtcggtcga tgccggcggg tccgcgcgat ccgtacgatc gaggtggccg cgggcctcga
      241 cccggcgtcc gaccaggacg ccgagcacga acaccatgcc gacgatgacg gcgccgccga
      301 agaacaggta gaagatctgg cgaccgtcga ggctgacctc gatcttgtcc ttgtagagct
      361 cgggctcgcg actgggcatg ccccagcctc ggcaactccg gtcaccgggg caagaaaacc
      421 gcctgagcct ggctcactgc agggtgttga cgatgaccgt cgtgctgccg tccgtggtcg
      481 caggcggcac cgactcgatg cggaacaccc ggccgcgggt cgagacatcg acgaacgtgg
      541 tggccaggaa gtcgccgtcg tcgatcaccc agatgctctc gctcgggccg cggaccacct
      601 tgaccggcga gacctggccg acgagcgcca cggtgagcgg cgtgaagccg gccttcacgg
      661 cgaacgtgac ctgatacccc gggaccacca gcgggatgtt ggtcgggatc ggcatctggg
      721 tctggctgtc gaccggatcg cgcccgaggc acgtctgtcg tgcttcgcgg tacgggtcca
      781 cgatcgtcaa ggtcagcgag cggttgcgga acttgatcgc ggggaagttc gcgcgctggc
      841 ccggcttcgc aggctgcgcc gggccgcggc acgtgccgta gcgcggcggc ggctggttgt
      901 cggagcacag cggcccctgg ccatcggagc acggcggtgg ctgttgctgg tctggcaggg
      961 gtagcttgcc atcggggccc gggcacgcca gcggatggcc atcgaggcac gacgacgggt
     1021 agctcggcgt ggtctcgaac tgcggcacgc tcagcgagca ggggttcggc tcgaacgtcg
     1081 cgctgtcgag ccggccggtg aggggatagt tgagtacgcc cagaggatcc gtcggcggcg
     1141 ggccgcacgg cggggcggtg agcgggatgc ggccgatcga cagcggatcc ttggccaggt
     1201 tggggtcgcg catgcagcgg ctgccatccg gcgacacgat gatcgggtgg acgaagcccg
     1261 agtgatcgcc gacgacggtg aacgcctcgc cggcgcggac gacgtagcgc tggggcccgt
     1321 tgaggcacgc ctgcggcggc tcgacgccct ccatgcacac gccgcccgtg acgctggtcg
     1381 gatcgggcgc gcagatcatg ccgttggcgc agaccggatg gggtttgttc ggatccggag
     1441 gcatgaggtc gcagacctgg acgcagcgct tgttgcgcgc cggatcggtg ttgatcggct
     1501 tgcgcagcgg atcggccatg cagctccagt gcgtcggccg ctgcgtcacc ggcgtgccgg
     1561 gcgcagcctc ggcgaacgga tccgcgccct ggttgaggac gaccgccgcc cgtgccaggt
     1621 cgtcgcactg atcgtcgctc acgcagccgt cgaccggcgt cgtcgacagc tcgtgcttgc
     1681 gctgccacag caccagcttg ccgggcgccg tgccgccgct ggcgtcggtg cccaccgtgt
     1741 agcgccggat cgagctgagg aagtcccggc aggcgtcctg cagccgcggc gcctcgctct
     1801 tgaggaagca cgcgccgacg ccgacggtgc tcagcgggtg gacatagcag gtgtagcccg
     1861 acgggcagtc ggtatcgatg ttcgccggat tgcagccctg gaactcgacg atgtcgccgg
     1921 gctcgacgcc catgctgcag aacggccgcg acgcgtccgc gatgaacatc ccggtgccgt
     1981 cgacgaaggt ctgaccgaag tgcagcgacg ggccgttgat cgagttgagc gtggagtcga
     2041 gctccagcgt accctcgtag gtcagcttcc agttctcgag ggccacgcct tcctggggcg
     2101 tccagttctt gaggtccacg acgctcttga gatcggggta cgccaggtcg cgcgagggcg
     2161 cagggccgtc cgacgcgctg gagcggatct cgggcgcccc gaaatccagc ggggtgaccg
     2221 ccacgtcgct gttcacgtcg cactggacct gctgcagccg ggggagctgg aggttgaggt
     2281 cggtgaacag cgtggccgcc acggcctgct ggaccggagg tgcggtgagc cggggcccac
     2341 ccgcgaacgc cccgcccttg ccatcggtgg tcgtgcagtc ggtggggggg tcctcgcggt
     2401 ccgacaccgc gtcgcgcagc tggtgcggca tcaccagcac cggggaggtc gcgagcgcgt
     2461 ggtccttggt cggggtgatg ttgtgggaca cgtagtcatc gttgacgttg gccacgaaga
     2521 ccgtgccgct cgacgccgtg atgaacgcga agtagccgag cagcagggtg ggctgcgcgc
     2581 ccacgaggtt cgcggtcggc ttgccgtcct gtccgatgac cggcggcggt gcggcgaggc
     2641 ccttgacgat cgtcaccgag ttcgcgacgt cgccaccggg cagctcgatg ccggggccct
     2701 gggcgcccgg ccggcgcggc aggttgggct ggcccagcgg gggacactcc agcgtggtgg
     2761 ccgaggtact tcgcacgaac cgcccgtcga tctgggtatc gcactcgcgc ttcagcttca
     2821 ggacgtcggc gacgcggacc gtgccgtcgg tcgccaccgc atagacgtac tgcgcctggt
     2881 catcggcctc gtcgttcact tttccgccgc ccgtgttgct gcccatgccg atctggggcg
     2941 acagcgccag cgcggagatg ccgaccggac cgcggttggc ctcgagcgga atctgcagca
     3001 ccgagcccga ccgcggtgcg aacgtcgtca gatcgaggtc gacgaccgtg agcggcgccg
     3061 cgaggggcgc tccgcggatg tcggcgccga tcgccagctt gctggtgatg tcgggcttga
     3121 cgccgttcgt gccgaacacg tcgcggtcgg tgcgcgtgtc gagcttgtgg tcgagcgcga
     3181 ccggccgtac gccgtccttc gcggtcacga ccggcatccc gcggggcggt gcgcactcgg
     3241 tcgggcagct ctggatcttg gccgggagcg tctggtcggt gagcacgctg gcggtgccgc
     3301 tcgggtcgct cgggtcgaag tagatgcccc cgatgacctg agcagtggcc ttgccgtccg
     3361 cgccggcggc gagccggacc tcggcgacga ggtggcagct cgggtaggcg atgtagacct
     3421 ggctggtcgc ggctttcggg cacgcgttgc cgaccttgtc ggtccccggg gagacgtcga
     3481 ggaacggctc gacgatcatc gccgacggct tggccaggat cggcgtgccg tccggcccgg
     3541 tgaccgtcac gcggtcgatc cgcggccgga cggtgctgtg atcgacgatg ttggcgagcg
     3601 tgctggtgat gtcgagctcc gagagatcgc acgagccggc gttggccgtg acctcgaagc
     3661 agccggtctt gtcggtgatg atcgcgaccg ggtcctcccc gatgctgatc gcgttcttgc
     3721 ccggggtcaa cggatcggcg tcgagcacct ggaacttgct ggtcggcgta tcgctgatct
     3781 gctgcggcag cgaccccgac gcgaacatgg agtccgccgg ggtcaccggc caggtcgtga
     3841 gcgccaccgt gccggacgcc gactggagga tgaagccgta ccagacgggg ttgggattgt
     3901 cgggggcggg attgtccttg gtgccgagcg tttcctggcc gaccaccggc acgaccggat
     3961 cggtgctcaa cgggggcgac agcgcctgac agacctccgt gggcatcgct gtcttgatga
     4021 tcgggtccgg cagcatcccc ggccccgggc ggccgttggt ctgccgcatc ttgccgtagc
     4081 aggcgaacga gacatcgacc gggcggtcga ggttgagctc gatcacgccg gtgttcgtgg
     4141 tgttgccgca cgcgccccag agcgcggcga ccgaggcgac cgcggcggac gcggcgagct
     4201 gccggccgcg ggtcactgcg gcctcggttg gccgatccgc agcacgacgc ggttgcgggt
     4261 gggggagctg gggtcgatgt cgatcacggc gatggtgtct tccaggaagt tcgagacgaa
     4321 caggaacttg gtcggcggcg gcagggtcgg cgacgcagcg agcgatagca cgtccgccgc
     4381 gtacgggccc cggccgaccg tgatgatgtc ctcgacctgc gactggccga acgggttcac
     4441 gacgtagacc tggccggtat cgaagcacgt gacgtagacc cgctcgtccg tgccggcgcc
     4501 ggccacggcc gccgccgagc cctcgcggca caggtcgctc gagccgagca gccggttgtt
     4561 cggcgtgcca tcgggaccga tcgacgtgtc gtagacctgg agcgacggcg gcgtgcgatt
     4621 cacgaggtac agccgcgtgc cgtccgccga gaaccggagg ccccggctgt cgaccgagct
     4681 gccggaggtt tcgccgacgg cgctgagcac gaagaacctg cccggcagga ggtagtccgc
     4741 ggcgccggcg cgcatgccga cgctgaattg ctgcacccgg ttgtcggtgt cgctcgcgac
     4801 gtacagcgtc tccggcgcca gctcgggccc tgccccgggc gcgggcgatg ccgggatctg
     4861 catcggcagc ccggcgacgc tggtcgcgcc gcgcgcggcc gtgctcggat cgccgaacac
     4921 gccctggagg atgtcggcga tctggaccgg gccatcggcc ggggcgtcga tcagcgtcac
     4981 cgcgccgctg gcgaggtggg tgaccatggc gaacccgtgc tggggagcga ccgtatggtc
     5041 gagggggagc ggcttgccca tggcatcgac cgggtgaacg acatcggcga acaccgcgaa
     5101 cggctcgttg ggcagcacct cgaggttcgg gtcgttgttc agcgaggtca ggcggtgggc
     5161 atcgtcgcac agctggtagg aattcgagcc ggcggtgcag tgcagggccg tgccgtcgaa
     5221 gtcggcccac gcgatcgagg gatccccgcg gctcggaacg aacacgcgca ggtgggtggt
     5281 tgccccggcg ctgaagtcct ggatcgacag atcggtggcg aagttgccga gcttgacgcc
     5341 ggcgtcggtg ttgatgaagt acgactcctc gcagcccagc gtcgcggcgt tgtccgggtc
     5401 gcacttgcag tccagcgcgt tgcccccgcc gggatccgga accttcggag gcagcgtcat
     5461 ctgcgcgaac gtgttgcacg ggtccggcgt gttcgacggc tgggtgaggt aggtcgtcca
     5521 ctccgagatc acgcgctgga cccggtcgag ggcgatcacg tcgagcgagc ccgagtcgaa
     5581 gcgcagctcc gagttggcgt tgacgacgaa cagcgccttg ccatccggcg acaccttcac
     5641 gccggtgggg aacgccagct cgtcgcggtg gagctcgata tcggagccgg acgcggtgca
     5701 cccgatcgcc gcaccgcaca ccagtgccca ggccgggccg cgccaggccg acctggcggt
     5761 gcgcgccggc gcagcaatcc tcgtggccat cctcatggga tgccgcttcg accgcgcccc
     5821 accccggggg gttgcctgcg gggatccggg gagcggtgct ggttctgttg cgtcgatcac
     5881 gtccatcacg gttcgttcac ttctttccgc tgggcgcatc ggacagggcc gcgcgcgccg
     5941 ccgcgacacg cgcgatcggg acccggaacg gcgagcacga tacgtagttc aggccggcgc
     6001 ggtggcagaa cccgaccgag gtcggatcgc cgccgtgctc gccgcagatc ccgaccttga
     6061 ggtcgggctt gacgctgcgc ccgccgcgga ccgcgagctc gatcagcgcg ccgacgccct
     6121 cggggtcgag cacggcgaac ggatcattcg ccagcacgcc ggcgtcgagg tacgccggca
     6181 ggaaccgccc ggagtcgtcg cgcgacagcc cgtaggtggt ctgggtcagg tcgttggtgc
     6241 cgaacgagaa gaagtcggcg tgggccgcga tctggtcggc gaccacgcag gcgcgcggca
     6301 gctcgatcat cgtgccgatc gtgtagggga tactcgcacc accgagcgcg gcgtcgaacg
     6361 tcttgccgac cagcgcgcgc agccggtgca gctcctcggg gtgcatgacc agcgggatca
     6421 tgatctcggg gtgaactgcc ccaccggccg cggcgacctc ggccgcggcc tcgccgatcg
     6481 cgcgggcctg gatctcgtag atctcgggga acgtgatcgc gagccggcag ccgcggtggc
     6541 cgagcatcgg gttggcctcg gtgagctcgg cgaccttggc cgcgacctgc cccgcgggct
     6601 tgccgagcgc cgcggcgacc tcctcgatct cggcctggcc gtgcggcaga aactcgtgga
     6661 gcggcggatc gagcagccgg atcgtgaccg gcaggccgtc catcacccgg aacagctcgg
     6721 cgaagtcgcc gcgctgcatc ggcaggatct tggccagcgc ggcgcgccgc ccggcctcgt
     6781 cgccggcgag gatcatctcg cggaccgcga ggatccgctc gggctggaag aacatgtgct
     6841 cggtgcggca caggccgatg ccctcggcgc cgaagctgcg cgcggtgcgc gcgtcggtcg
     6901 gcgtgtcggc gttggtccgg acctggagcc ggcggaacga gtcgacccac gtcatcagcg
     6961 tgccgagctc gttgccgagc tcggccggga ccagcggcgc cgagccgagg aacacctcgc
     7021 cggtcgagcc gtcgagcgtc agcgtgtcgc cctccttgat cttgcgctcc tggccgccgc
     7081 cgacgaacga cgccgtgcgc ttggccgcct cgacccgcag cgacgtgcac gacgtcacgc
     7141 agcacttgcc catgccgcgc gcgacgaccg cggcgtgcga cgtcatgccg ccgcgcgagg
     7201 tcaggatccc ggcggccgcc ttcatgccgt ggatgtcctc cggcgaggtg tcggtgcgga
     7261 ccagcagcac ggtctcgccg cgctccgccc agtccttggc cgcgttggcg gtgaacacga
     7321 cccggccgat cgcggcgccg ggcgacgccg gcaggccctt ggcgatcgcg ggcggccgcg
     7381 ccttggggtc gatcgtcggg tggagcacct cgtcgagctt ggccgggtcg atccgcagca
     7441 ccgcgtcgcg cggcgtcagc ttgccctcgc cgaccatgtc gaccgcgacc ttgaccatcg
     7501 ccttgccggt gcgcttgccg ctccgggtct gcagcatgaa cagctcgccg gactggatcg
     7561 tgaactcgag gtcctgcatg tcgcggaagt ggtcctcgag cagcttggcg acccggacca
     7621 gctcggcgtg ggccctgggc agcttcttgg cgagctcgct gatcggctcg ggcgtgcgga
     7681 tcccggcgac cacgtcctcg ccctgggcgt tggtcaggaa ctcgccgtac agctcggcgg
     7741 tgccggtcgc aggattgcgg gtgaacgcga ccccggtcgc gcagtcgtcg ccgaggttgc
     7801 cgaacaccat ggcctggacg ttgaccgcgg tgcccatcgt cgacgggatg ccgtgcagct
     7861 tgcggtagta gtcggcgcgc gggttgttcc atgaccggaa caccgcgccg atcgcgcccc
     7921 acagctgggt ggtgacgtcg tcggggaacg tctggccggt gatctgcttg acctcggcct
     7981 tgaaccgggt gaccagctcg cgcagcgcgg cctcgctgag gtcctggtcg cgcgccgcct
     8041 tgtgcgcgtg cttgacctcg tcgaggatgc ggtcgaacgg cggctcgtcc tgctcgctgg
     8101 gcgcgaccac gcccatcacg acctcgccgt acatcgcgac gaaccggcgg tagcagtccc
     8161 acgcgaaccg cgggttgccc gagcgctggg cgagcgcggc gaccgtggtg tcgttgagcc
     8221 cgaggttca
//
LOCUS       DASVOR010000505         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_326066,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000505 DASVOR010000000
VERSION     DASVOR010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326066"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1185
                     /locus_tag="VF516_10110"
     CDS             172..1185
                     /locus_tag="VF516_10110"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEX8108070.1"
                     /translation="MPTMKAVQVSKPGGDFELVEREIPRPGPGQVRIRVQACGVCHSD
                     SVTKDGLFPGIQYPRVPGHEIAGVIDEVGPGVVSWRKAERVGVGWHGGQDGTCPACRR
                     GDFANCASGIVCGISYDGGYQEYMVAPVEAVARMPESLDAAEAAPLMCAGITTFNALR
                     HSGAGPGDLVAIQGIGGLGHLGVQFARKFGYRVAAIGRGAQNQALAKQLGADVYIDGA
                     AVDAATELQKLGGARAIVATAPSAKAMSALIPGLGPNGALLVVGVSPDPIEVPPMSLI
                     AGRKRVQGWASGTPADSEDTLWFAEATGVRPKIERYPLARAAEGYARMMSSKAEFRVV
                     LTM"
     gene            1340..>1574
                     /locus_tag="VF516_10115"
     CDS             1340..>1574
                     /locus_tag="VF516_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108071.1"
                     /translation="MVALGIGLLSPGCKQAGSAEPVTARSAASASAPTADRVETAAAP
                     ARSETVVTGAAGSAAGKPSSVVAVEAPAKDGARA"
BASE COUNT          225 a          549 c          604 g          196 t
ORIGIN      
        1 cgctgccccg gaagccgacg cccggcgggc gcgggcgcgt caccgtcgag ccgctccgac
       61 gatcgcggcg accgcggccg ctcgccggac gcggcccatc gcgtgccccg cggtggcgcc
      121 tcgcgggcat cgcgacgagc cgcgagcacc cacgaatcag aggaagacca gatgccaacc
      181 atgaaagcag tgcaggtctc gaagcccggc ggcgacttcg agctcgtgga acgcgagatt
      241 ccacggccgg gcccggggca ggtgaggatc cgtgtccagg cgtgcggcgt gtgccacagc
      301 gactcggtca ccaaggacgg cctgtttccc gggatccagt atccgcgggt gcccggccac
      361 gagatcgccg gcgtgatcga cgaggtcgga ccgggcgtgg tcagctggag aaaggccgag
      421 cgggtcgggg tcggatggca cggcggccag gacggcacct gtccggcgtg ccggcgcggc
      481 gacttcgcga actgcgcgag cggcatcgtg tgcgggatca gctacgacgg cggctatcag
      541 gagtacatgg tcgcgccggt cgaggccgtg gcgcgtatgc cggagtcgct ggacgccgcc
      601 gaggccgcgc cgctgatgtg cgccgggatc acgacgttca acgcgctccg ccacagcggt
      661 gccggcccgg gcgacctggt cgcgatccag gggatcggcg ggctcggtca cctcggcgtg
      721 cagttcgccc ggaagttcgg ctaccgggtc gccgcgatcg ggcgcggcgc gcagaaccag
      781 gcgctcgcca agcagctcgg cgccgacgtc tacatcgacg gcgccgcggt cgacgccgcg
      841 accgagctgc agaagctcgg cggcgcgcgc gcgatcgtgg ccacggcccc cagcgcgaag
      901 gcgatgtcgg cgttgatccc cggcctgggc cccaacggcg cgttgctggt cgtcggcgtc
      961 tcgcccgatc cgatcgaggt cccgccgatg tcgctgatcg ccgggcgcaa gcgcgtccag
     1021 ggctgggcgt ccgggacccc ggcggactcg gaggacacgc tgtggttcgc cgaggccacc
     1081 ggcgtgcggc cgaagatcga gcgctatccg ctcgcccggg ccgcggaggg ctacgcccgc
     1141 atgatgagca gcaaggccga gttccgcgtc gtgctcacga tgtgaggtgc gcccgtgcgg
     1201 tctgcgacac gcgcgccgcg gtcgagcttg gaagaaaact gtcatcgccc gatgacagga
     1261 tttcacgatc tcgtgcagac cggccgcgcc cgacgcgcgt tcgggaggcg caggaggatc
     1321 catgcgcaat acgctcccga tggtggctct cggaatcggc ctgctctcgc caggttgcaa
     1381 gcaggccggg tcggccgagc cggtcaccgc ccggtcggcg gcctcggcca gcgccccgac
     1441 ggcggaccgt gtcgagactg cggcagcgcc cgcgcgctcc gagaccgtgg tcacgggtgc
     1501 cgccggctcg gccgcgggca aacccagcag cgtggtcgcg gtggaggcgc cggcgaagga
     1561 cggcgccagg gcga
//
LOCUS       DASVOR010000506         3096 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_92636,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000506 DASVOR010000000
VERSION     DASVOR010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92636"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="VF516_10120"
     CDS             complement(<1..754)
                     /locus_tag="VF516_10120"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX8108072.1"
                     /translation="MRYWFLAILTLPLIARADDVATRAQKIHASSIVVDTHLDAPDQL
                     SSKWADVATRGATDHFDIPRARQGGLTAPFFSIYVASSYADHGAARRALELIDLTHRV
                     VDGHRQDMTLATTPADIRAAKQAGKIAVLMGIEGGHAIEDSLGVLREMYRAGVRYMTL
                     THVNTNHWADSSGPFYEPDFDPRQSAVHHGLSQFGKTVVKEMNRLGMIVDISHVSDDT
                     IRDVLAVSRAPVMASHSSCRALSSIPRNLTDDQ"
     gene            784..1050
                     /locus_tag="VF516_10125"
     CDS             784..1050
                     /locus_tag="VF516_10125"
                     /inference="COORDINATES: protein motif:HMM:NF013083.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HEX8108073.1"
                     /translation="MSDLAQRFADAQARIRQVSSLGNDVLLELYGLYKQATAGDASGS
                     RPGMLDIRGRAKYDAWAARKGTTADAAMTAYIAVVDRATRPAVR"
     gene            complement(1275..2243)
                     /locus_tag="VF516_10130"
     CDS             complement(1275..2243)
                     /locus_tag="VF516_10130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108074.1"
                     /translation="MEETMAIAHANDTGALGDMVRGAGAAHCGTGLHVYHASYVNLVL
                     AARSERGTVTLVAGSHTRSFPYFETEPWDNCLLQIGDAGYTFTGRVTGAALHLELDPD
                     AAEPRGAIRFTGEMRRASDGAAVTIELDVAIALTPYPTRLLGEPYNFLHLDRMLGLAW
                     TPYQLTGERGRVRIDGTELVLEGICGVCEHGRLTNLRSPAFAIKYDYVAVAYPGDDGY
                     GLIRFTSHALHGDGVLGHALDWYLRRSASALLTIEPSKLTDGNPRGVDIPPQDDPAVL
                     RFENEVDLGPAVLRRQMIETRDRAGHVLYGLREIFVAKPAAAPLPP"
     gene            complement(2359..2883)
                     /locus_tag="VF516_10135"
     CDS             complement(2359..2883)
                     /locus_tag="VF516_10135"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8108075.1"
                     /translation="MIVQLDDVHLPVLVAHIARHSRESGRGGDLIFRPRSSDAPIDEP
                     AAIERHRAGWALAIDEPYWLRTWGVVVDGQIRGHLDLHGGRLPSEFHRAMLGMGVERP
                     WRGKGHGRALLATAIGWARGAGLAWLDLGVFAHNHRARKLYASVGFVELGTTRDRYRV
                     DDAVIDDVAMALKL"
     gene            2901..>3096
                     /locus_tag="VF516_10140"
     CDS             2901..>3096
                     /locus_tag="VF516_10140"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8108076.1"
                     /translation="MTQSTWQLRGRNALVTGGTRGIGRAIVAELLELGAHVALVARSP
                     AEVDAAVRELGDRVTGVAGDV"
BASE COUNT          449 a         1066 c         1140 g          441 t
ORIGIN      
        1 tctggtcgtc ggtcaggttg cgcgggatgc tcgacagcgc gcggcacgac gagtgcgacg
       61 ccatcaccgg cgcgcgcgac accgcgagca cgtcgcggat cgtgtcgtcc gagacgtgcg
      121 agatgtcgac gatcatgccg agccggttca tctccttgac cacggtcttg ccgaactggc
      181 tgagcccgtg gtggaccgcc gactgcctgg gatcgaagtc gggctcgtag aacggccccg
      241 acgagtccgc ccagtggttg gtgttgacgt gggtcagcgt catgtagcgc acgccggcgc
      301 ggtacatctc gcgcagcacg ccgagcgagt cctcgatcgc gtgcccgccc tcgatgccca
      361 tcagcacggc gatcttgccg gcctgcttgg cggcgcggat gtcggcgggc gtggtcgcga
      421 gcgtcatgtc ctggcgatgg ccgtcgacca cgcggtgggt gaggtcgatc agctccagcg
      481 cgcgccgcgc ggcgccgtgg tcggcgtacg acgacgcgac gtagatcgag aagaacggcg
      541 cggtcagccc gccctgccgg gcgcgcggga tgtcgaagtg atcggtcgcg ccgcgggtcg
      601 cgacatcggc ccacttgctc gacagctggt cgggcgcgtc gaggtgggtg tccacgacga
      661 tcgagctggc gtggatcttc tgggcgcgcg tggcgacgtc gtcggcgcgc gcgatcagcg
      721 ggagggtcaa gatagcgagg aaccagtacc gcatgcggcg cagtgtatcg atatgctcgc
      781 cgcatgtccg atctcgcgca gcggtttgcc gacgcccagg cccggatccg gcaggtctcc
      841 agcctgggca acgatgtgtt gctcgagctc tacgggctct acaagcaagc gaccgccggc
      901 gacgcgtccg gcagccggcc cggcatgctc gacatccgcg gccgcgccaa gtacgacgcc
      961 tgggccgctc gcaaggggac gaccgccgac gccgcgatga cggcgtacat cgccgtggtc
     1021 gaccgcgcga cgcgccccgc ggtgcgctga cccgccggtg ggggcgagcg cgagcgcgac
     1081 gtccggaact ccggccccga tcaccgtgaa ctcgactact tgctcgggca agaggcgccg
     1141 ggaagccggg gatcgatcgc gcaactcgat gtgatgaccg gtgaattcgg ccggccttgc
     1201 gacgcctgaa cagggaacga ccggccggtc ggccgcggca gccgccttgc ggcagcgcgt
     1261 tgaccggtgt gcggctaagg tggaagcggc gccgcggcgg gcttggcgac gaagatctcg
     1321 cgcaggccgt acagcacgtg gccggcgcgg tcgcgggtct cgatcatctg gcgcctcagc
     1381 accgcggggc cgaggtcgac ctcgttctcg aaccgcagca ccgccggatc gtcctgcggg
     1441 gggatgtcca cgccgcgcgg attgccgtcg gtgagcttgc tcggctcgat cgtcagcagc
     1501 gcggacgccg acctgcgcag gtaccaatcg agcgcgtggc cgagcacgcc gtcgccgtgc
     1561 agcgcgtgcg acgtgaagcg gatcaggccg tagccgtcat cgccgggata cgcgaccgcg
     1621 acataatcgt acttgatcgc gaaggcgggc gaccggaggt tggtcagccg gccgtgctcg
     1681 cacacgccgc agatgccctc gagcacgagc tcggtgccgt cgatccgcac gcggcctcgc
     1741 tcgccggtca gctggtacgg cgtccacgcc aacccgagca tgcggtcgag gtggaggaag
     1801 ttgtaaggct cgccgagcag ccgggtcggg tacggagtca gcgcgatcgc gacatcgagc
     1861 tcgatcgtga ccgcggcgcc gtcgctggcg cggcgcatct cgccggtgaa ccggatcgcg
     1921 ccgcgcggct cggccgcgtc gggatcgagc tccaggtgga gcgccgcgcc ggtgactcgc
     1981 ccggtgaacg tgtacccggc gtcgccgatc tgcagcaggc agttgtccca cggctcggtc
     2041 tcgaagtacg ggaagctgcg tgtgtgcgag cccgcgacca gcgtcaccgt gccgcgctcg
     2101 ctgcgggcgg cgagaaccag attgacgtag ctcgcgtgat agacgtgcag gccggtaccg
     2161 cagtgcgccg ccccggcgcc gcgaaccatg tctcccagcg cgccggtgtc gttggcgtgc
     2221 gcgatcgcca tcgtctcctc catcccatcc tcctcgttgc aacccctcga tctgatcgca
     2281 acgccgcgcc gtgatcaaga tggacggatg gcaaacttca caggtgcgac ctgcacgtgg
     2341 ccgaccgatg ggccccggtc acagcttcag cgccatcgcg acgtcgtcga tcactgcgtc
     2401 gtcgacccgg tagcggtcgc gcgtggtgcc gagctcgacg aagccgaccg aggcgtacag
     2461 cttgcgcgcg cggtggttgt gggcgaacac gccgaggtcg agccaggcca gcccggcgcc
     2521 gcgcgcccag ccgatcgcgg tggcgagcag cgcgcggccg tggcccttgc cgcgccacgg
     2581 gcgctcgacg cccatcccga gcatcgcgcg gtggaactcc gacggcagcc ggccgccgtg
     2641 caggtcgagg tggccgcgga tctgcccgtc gacgacgacg ccccaggtcc gcagccagta
     2701 cggctcgtcg atcgccagcg cccagccggc gcggtggcgc tcgatcgcgg ccggctcgtc
     2761 gatcggagcg tccgacgacc gcggccggaa gatcaggtcc ccgccccggc ccgactcccg
     2821 gctgtggcgg gcgatgtggg ccaccagcac gggcaggtgg acatcatcga gctgcacgat
     2881 catcgggata tagtgccacc atgacgcaat cgacgtggca gctgcgcggg cggaacgcgc
     2941 tcgtgaccgg aggcacacgc gggatcggcc gcgcgatcgt ggccgagctg ctcgagctcg
     3001 gcgcgcacgt cgcgctggtc gcgcgctcgc cggccgaggt cgacgccgcg gtgcgcgagc
     3061 tcggcgaccg ggtgaccggc gtggccggcg acgtga
//
LOCUS       DASVOR010000507         5568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413174,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000507 DASVOR010000000
VERSION     DASVOR010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5568
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413174"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VF516_10145"
     CDS             complement(<1..314)
                     /locus_tag="VF516_10145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108077.1"
                     /translation="MDQLVGLGDREPLRLPRSFAMDAEQGEPQLPRGCTAKAEVSASD
                     RGLEIEDMKRLLCRAQVKFGGASALSSALEVPGQDHGIAFSALLEPLAGAAVREPLIV
                     GG"
     gene            351..1397
                     /locus_tag="VF516_10150"
     CDS             351..1397
                     /locus_tag="VF516_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108078.1"
                     /translation="MLSYHLRALMERRIEDLGEPLALLQEVTPIPGAAGAISLMFLTR
                     VYVLDSFGHIAQATALEEPFRALVRSRAEQEPLASVWWHIALGMRSSHAYDDPWSALQ
                     HGDAIQPICDAIGGQIIFVNMQLARGINQWYLGALGSAMQTLEGIPAADTGVGMASSL
                     RRFSLSWLYADCGALDPAYALASQLVESGHARDNHLEEARGRWVLAEVLRRLGDLDAA
                     EREIQTALAMAMPLEVPGVLATRVGLRVAQGRAAEALAAAEEAMARYTAMGGCGVFRG
                     AFVRLAHAEALHATSAHDAARGAIAQARDRLFAIAARIADPDYRTSFLEQVPENARTL
                     ALASAWLGGPAPSA"
     gene            complement(1466..3109)
                     /locus_tag="VF516_10155"
     CDS             complement(1466..3109)
                     /locus_tag="VF516_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108079.1"
                     /translation="MDVEISLYSIHCFNSGERGHAEPYLWTTFFKIDDSVVKQRTDDP
                     RFVSGTPEIVFGQGSHGNLGAPMATSDTRAIDTTVGRYLTSLGNLTIKNPLDGTTILL
                     PGIVGAVVLLMEENSVSDDGAVAAHRACNALLAEKIPAIVRGIDMVQLYLTAVQLQSS
                     KNLTLIEALTQALSQLVQAQVPGLVEAAKQRIKDAVKAAQNVFQDISAAADSDDVIDF
                     QILYHASTELVAPSFDVEFSLKFQEAGQTSVSGGGGVTPNLGLKSYRIDGRFKRAAGT
                     DRSLALGGTLTSGPCVCSRGENRLDVFARGADHSLVHKLWNGSKWSDWEGLGGQLTSD
                     PAAVSWASDRIDVFARGLDNSLYHLYWNGKWSNWQGLGGQLTSSPAVCSWAKDRLDVF
                     ALGTDGSLYHKYWNGSKWSGWEGLGGQLSSDPAAVSWGSDRIDVFARGMDNALYHKYW
                     NGSKWSGWEGLGGQLSSGPAAASRGAHRLDVFGRGTDGSLYHRLWDGSAWSSWEGRGG
                     VLTSDPDAVAWSSQRLDIFFRGSDQGLHQIWWNSGIWHP"
     gene            3374..5152
                     /locus_tag="VF516_10160"
     CDS             3374..5152
                     /locus_tag="VF516_10160"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8108080.1"
                     /translation="MCIDRLTRVRRAAAAGLALATAIGLACRSSAPVPPLVPATAELV
                     VDDELLRGLRPQPWLDSPGTSEQYFPIFLGASVRDLVLGRPPAPRPRLPVALRDLGLD
                     QARRIAGTLPGRRVELEVWPTQDWNGQPTWVFARWRSSAGAAWTWLTETRNGRPAGTQ
                     VTGPGPLLPERIDPSNSPGREALFSDFLASSDLYDQPRFGNCVYVRRSSGAIALAWPD
                     PRERDARAAELTRPGAFDAYCVNQPARFNDRPIAFVVAIAGGAIVWPAEIDWVPAPSE
                     RPAPAQAALQVAGWPGDFAARYQEDLQIADGEREARYPLSGRTGRVRIKGSFQPDHDL
                     ERMVDYLEERYRVLSIETVRQRFTWRGIAQSNLIAIIQGTEGGPPVVMADHIDTACEE
                     DTFAATHRRVTTHGADDNATATAALLGAAAVLRDAHPRHDIWLVHLTGEEFPSDDLGA
                     RHFLSEMRRAHQDIRAAVITDFIGWHRPGKPAFQVSPTSLPGSERMAALALDAARQLA
                     PELSVIYAARDELRNSVFQTDLQEFELHGIPGILFNEDMDYSDRSTGNPHNHQSTDVA
                     STLDLPFAAAVAKIAIETTLRLANDD"
     gene            complement(5149..>5568)
                     /locus_tag="VF516_10165"
     CDS             complement(5149..>5568)
                     /locus_tag="VF516_10165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108081.1"
                     /translation="LARELGATPEDGAPPEAIVRVAVAHGLSATKREHMTVDDLAAEI
                     ARGNPVIVELQAWSDARRTSWVDDWDDGHYAIAIAVEGDRVVFEDPSVLGSRAVLSRR
                     ELEERWHDIDAGYRNVRAGIVFGGKPPAPPRAQVHMD"
BASE COUNT          798 a         2034 c         1904 g          832 t
ORIGIN      
        1 tgaccgccga cgatcagggg ctcgcgcacc gccgcgccgg cgagtggctc gagcagcgcg
       61 gagaaggcga tcccatggtc ctggccgggc acttcgagcg cggaggacag ggcgctcgcg
      121 ccgccgaatt ttacctgcgc gcggcagagc aggcgcttca tgtcctcgat ctcgaggccg
      181 cgatcgctcg cgctgacctc ggccttggct gtgcaccccc gcgggagctg cggctcgccc
      241 tgctcggcgt ccattgcgaa gcttcgcgga agtcgcagcg gctcacgatc gccgaggccg
      301 acgagctgat ccacgcggcg ccgcgcggct ccctcccctg ggcgcggggg atgctctcct
      361 atcacctgcg cgcattgatg gagcgccgga tcgaggatct cggggaaccg ctggcgctgc
      421 tccaggaggt caccccgatc cccggggcgg cgggggcgat atcgctcatg ttcctcacca
      481 gggtctacgt cctcgacagc ttcgggcaca tcgcgcaggc caccgcgctc gaggagccgt
      541 ttcgcgcgct cgtccgctcg cgggcggagc aggagccgct cgcgagcgtg tggtggcata
      601 tcgccctcgg catgcggagc tcccatgcgt acgacgaccc ctggagcgcc ctgcagcacg
      661 gcgatgcgat ccagccgatc tgcgacgcca tcggcggcca gatcatcttc gtgaacatgc
      721 agctggctcg agggatcaac cagtggtacc tcggcgcgct cggatccgcg atgcagacgc
      781 tggaggggat cccggcggcg gacacggggg tcggcatggc gagctcgctg cgccggttct
      841 cgctatcgtg gctgtacgct gactgcggcg cgctcgatcc agcgtacgcc ctggccagcc
      901 agctcgtcga gtccggccac gcgcgagaca accacctgga ggaggcgcgc ggtcgctggg
      961 tgctcgcgga ggtgctccga cgcctgggcg atctcgacgc cgccgagcgc gagatccaga
     1021 ccgcgctggc catggcgatg cctctcgagg ttccgggggt cctcgcgacg cgcgtggggc
     1081 tccgcgtcgc ccagggccgc gccgcggagg cgctcgccgc cgccgaggag gccatggcgc
     1141 gctacacggc catgggcggg tgcggcgtgt tccgcggcgc gttcgtgcgc ctcgcccacg
     1201 ccgaggcgct ccacgccacc agcgcccacg acgccgcgcg cggcgccatc gcccaggcgc
     1261 gggaccgcct gtttgccatc gccgccagga tcgccgatcc ggactaccgg acgagcttcc
     1321 tcgagcaggt ccccgagaac gcccggaccc tcgccctggc gagcgcgtgg ctcggcggcc
     1381 ctgcaccgag cgcgtagatc ccggcgctgt ggatcgcgca gatttcgccc gctcgcggat
     1441 tcgcccgctc catggacgcg cagactcagg ggtgccagat cccgctgttc caccagatct
     1501 gatgcagccc ctggtcgcta ccgcggaaga agatgtccag tcgctgcgag ctccacgcga
     1561 ccgcgtcggg gtcggatgtc aggacgccgc cgcggccctc ccagctgctc cacgccgagc
     1621 cgtcccacag ccggtgatac agcgagccgt cggtgccgcg gccgaacacg tcgaggcgat
     1681 gcgcgccgcg cgacgccgcg gcggggcccg aggacagctg gccgcccagg ccttcccagc
     1741 cgctccactt cgagccgttc cagtatttgt gatacagcgc gttgtccatc ccgcgcgcga
     1801 acacgtcgat gcgatcggag ccccagctga ccgcggcggg atccgagctc agctggccgc
     1861 cgaggccttc ccagccgctc cacttcgagc cgttccagta cttgtggtac agcgagccgt
     1921 ccgttccgag cgcaaacacg tcgagccggt ccttggccca cgagcacacg gctggactcg
     1981 aggtgagctg gccgccgagg ccctgccagt tgctccactt gccgttccag tagaggtgat
     2041 acagcgagtt gtccagtccg cgcgcgaaca cgtcgatccg atccgaggcc cacgacaccg
     2101 ccgccggatc cgaggtcagc tggccgccga gcccctccca gtcgctccac ttcgagccat
     2161 tccacagctt gtgaaccagc gaatggtcgg cgccgcgggc gaacacgtcg agccggttct
     2221 cgccgcgcga gcacacgcac ggccccgacg tgagcgtccc gcccagcgcg agggaccgat
     2281 cggtgcccgc ggcccgcttg aaccgaccgt cgatgcgata cgacttgagc ccgaggttgg
     2341 gcgtgacgcc gccgccgccg gagaccgagg tctgcccggc ctcctggaac ttcaggctga
     2401 actcgacatc gaagctgggg gccacgagct ccgtgctggc gtggtagagg atctggaagt
     2461 cgatgacgtc gtcggaatcg gccgctgcgg agatgtcctg gaacacgttc tgcgccgcct
     2521 tgaccgcgtc cttgatgcgc tgcttggccg cctccacgag cccgggaacc tgtgcctgga
     2581 cgagctgcga tagcgcctgg gtcagcgcct cgatcagcgt gaggttcttg ctggactgca
     2641 gctgcacggc ggtcaggtac agctgcacca tgtcgatgcc gcgcacgatc gccgggatct
     2701 tctccgcgag cagcgcgttg caggcccggt gcgcggcgac ggcgccatca tcgctcacgc
     2761 tgttctcctc catgaggagc acgaccgccc ccacgatgcc ggggagcagg atcgtcgtgc
     2821 cgtcgagcgg attcttgatc gtcaggttgc cgagcgaggt caggtatcgg ccgaccgtgg
     2881 tgtcgatcgc gcgcgtgtcc gaggtcgcca tgggtgcgcc gaggttgccg tggctgccct
     2941 gtccgaagac gatctccggg gtgccggaca cgaaccgcgg atcgtcggtg cgctgcttga
     3001 cgacgctgtc gtcgatcttg aagaacgtgg tccacaggta cggctcggcg tggccgcgct
     3061 ccccggagtt gaagcagtga atgctgtaga ggctgatctc gacgtccatg ttcccatctc
     3121 cttctggccg gacatcgtat tccgatacag cccgattcaa cattaggatt taaatcggga
     3181 atgggacacg agcgacgaga tcgagcatgg ccatcgagcc aaggttgcca gtgcggaaac
     3241 cgccggattc ccgccgcaca aacgcctcgg cttcgccgcg gacgccgccg gcgcgactcg
     3301 ccggtcgcga gcacgctggg caggccgtgc ttgccatcgg cgctccgatc gggtaccgcc
     3361 tgcgtacccg accatgtgca tcgaccgcct cacgcgcgtc cgccgggccg ccgcagcggg
     3421 cctggcgctc gcgacggcga tcgggctcgc gtgcaggagc tccgctccgg tcccgccgct
     3481 cgtgcccgcc accgccgagc tggtggtcga cgacgagctg ttgcgcggtc tccgcccgca
     3541 gccgtggctc gactcgccgg gcacctcgga gcagtacttc ccgatcttcc tcggtgcgag
     3601 cgtgcgcgac ctcgtcctgg gccgaccgcc tgcgccccgg ccgcggctgc ccgtggcgct
     3661 gcgggatctc ggcctcgacc aggctcgccg gatcgccggg acgctgcccg gacgccgcgt
     3721 cgagctcgag gtctggccga cccaggactg gaacggccag cccacctggg tgttcgcgcg
     3781 ctggcgcagc agtgccggcg cggcgtggac gtggctgacc gagacccgca acgggcggcc
     3841 ggcggggacc caggtgaccg gccccgggcc gctgctgccg gagcggatcg atccgtcgaa
     3901 cagccccggc cgcgaggccc tgttcagcga cttcctcgcg tcgagcgacc tctacgatca
     3961 accgaggttc ggcaactgcg tctacgtccg gaggtcgagc ggtgcgatcg cgctcgcctg
     4021 gcccgatcca cgcgagcgcg acgcccgggc cgccgagctc acccggccgg gagccttcga
     4081 cgcctactgc gtcaaccagc ccgcgcggtt caacgatcgg ccgatcgcct tcgtcgtggc
     4141 catcgcgggc ggcgcgatcg tgtggccggc cgagatcgac tgggtgccgg cgccgagcga
     4201 gcgcccagct cccgcccagg ccgccctgca ggtggccggg tggccgggcg acttcgccgc
     4261 tcgctaccag gaggatctgc agatcgccga cggcgagcgg gaggcccggt atccgctgtc
     4321 gggccgcacc ggccgggtcc gcatcaaggg ctcgttccag cccgatcacg atctcgagcg
     4381 catggtcgac tatctggagg aacgctaccg ggtgctctcg atcgagacgg tgcgccagcg
     4441 cttcacgtgg cgcggcattg cgcagtccaa cctgatcgcg atcatccagg gcaccgaggg
     4501 gggaccgccg gtcgtgatgg ccgaccacat cgacacggcg tgcgaggagg atacgttcgc
     4561 cgcgacgcac cggcgagtga ccacgcacgg cgccgatgac aacgcgaccg cgaccgccgc
     4621 gctgctcggc gccgccgcgg tgctgcgcga cgcccatccg aggcacgaca tctggctggt
     4681 ccacctgacc ggagaggagt tcccgtccga tgacctgggc gcgcgccact tcctgtccga
     4741 gatgaggcga gcccaccagg acatccgcgc cgccgtgatc accgacttca tcggctggca
     4801 ccgcccgggc aagccggcct tccaggtcag cccgacctcg ctccccggct cggagcgcat
     4861 ggccgcgctc gcgctcgatg cggcccggca gctcgcgccc gagctgtcgg tgatctacgc
     4921 ggcgcgcgac gagctgcgca acagcgtgtt ccagaccgat ctacaggagt tcgagctcca
     4981 tggcattccc gggatcctgt tcaacgagga catggactac tccgatcgat cgaccggcaa
     5041 cccccacaac caccagtcca ccgacgtcgc gtccaccctc gacctgccgt tcgccgccgc
     5101 ggtcgcgaag atcgcgatcg agaccaccct gcgactcgcc aacgatgact agtccatgtg
     5161 gacctgggcc cggggcggag cgggcggctt gccgccgaac acgatgccgg cccgcacgtt
     5221 gcggtacccg gcgtcgatgt cgtgccagcg ctcctcgagc tctcgccggg acagcaccgc
     5281 gcggctgccc agcaccgatg ggtcctcgaa cacgacccga tcgccctcga cggcgatcgc
     5341 gatggcgtaa tgtccgtcgt cccagtcgtc gacccacgat gtccgccgcg cgtccgacca
     5401 ggcctggagc tcgacgatga cggggttccc ccgggcgatc tcggccgcca gatcgtcgac
     5461 ggtcatgtgc tcgcgcttgg tggcgctcag gccgtgcgcg accgcgacgc ggacgatcgc
     5521 ctcgggcggc gctccgtcct ccggcgtggc gcccagctcg cgtgcaag
//
LOCUS       DASVOR010000508         5093 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_560063,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000508 DASVOR010000000
VERSION     DASVOR010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5093
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560063"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="VF516_10170"
     CDS             complement(<1..557)
                     /locus_tag="VF516_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8108082.1"
                     /translation="MVILRTGRRRRLVRGALVAITAVNAALLAVLALALAVPLPDRRG
                     EWSAAIEYRDGTPAHVFLARDDKWRLPIALDDVDPALVAALIALEDKRFWRHDGVDPI
                     AIVRAAASDARHARRVSGGSTLTMQLARLLEPRPRTLRAKLVDMFRAVQLDLRLSKRE
                     ILAEYLSRTPYGGNVEGVESAAWSY"
     gene            1432..3132
                     /locus_tag="VF516_10175"
     CDS             1432..3132
                     /locus_tag="VF516_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108083.1"
                     /translation="MMKSLVFGVLVGTAAAAHADAKPTDLPAELKAIAAKYPPPKDQR
                     WQPAAGPGCTAPTAAERAAAMKRAFAWIDQQHPDEVGSNQVTNNTPEVEMNVGCKDAA
                     GVLVLDVSQDRDLKKKKKEDGFSERRRNYLLKLSAAAIDVIAEDTSDSTQAWSEWADE
                     GRISLLAQLDVDGDGALDIVYSDHQHEGGAKSTFDSVHVRFATGKTGEAGRVTNLVDR
                     KVVNKQLVIAGELREDARVYACLGADLHFSPCAASKALQAEADKRAIIDRFSQAAPNN
                     LPDREQLAMDLGALGTRAQRRAQLVAAAPETDPGTHVQRKAIAFLVNAGLLEPAPMPD
                     IFDQTHPEAQTYLDGLAAKLGDTTCATSPLSAEDTAKANAWIKKQDEKASEIKIEPAS
                     CGPYTWVSWWPPNNTDNKRREALLGRDGTRIIGMTYEPQPYNPQIPGDEGFSFTQQWF
                     SHDGAIVGIVIGSGNLFVISNGKAVASTKGNNLGLYHADDRFSESSVDVFVDGGTLWH
                     ATPTGRERLDMALVKDHEARRAAIALLVAGPAGNAAQYAAALELLGADKALVAEAKKL
                     "
     gene            complement(3520..3687)
                     /locus_tag="VF516_10180"
     CDS             complement(3520..3687)
                     /locus_tag="VF516_10180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108084.1"
                     /translation="MKKNNRKLGLHSETIRTLDNIDLKRARGGDDAARPTNGTQSGIN
                     CVAQDAVPVPK"
     gene            complement(3684..>5093)
                     /locus_tag="VF516_10185"
     CDS             complement(3684..>5093)
                     /locus_tag="VF516_10185"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8108085.1"
                     /translation="IDAVAFSPDGRLAVTGSHDGTVQVWDATSGTLRSRSNELRSDVL
                     SIEFDQTSSLIAASSASGSIAVIEATSGMPVTELDGPTAKVRAAHFDPSSRRVVGASW
                     DGTARIWDATAMYRRWGSPFNDEDCGIITSLEPDRRFLAIRCNDEPTRIWDTAQNRLV
                     AELPFVTPIGDGFAPIYPAVSAAGDCAAIARGNDVEVYELPTARLLRTVKHGASVSAV
                     AFAAAGRDIVSGAIDGSVIVTRDNGTLLMLPTAPAGIDTAGFLSDGRIVVVDAGRHLR
                     IYDLGGATLADFETQTRVGTLRMSSDGHRLVTLPIFPGKVAAPELWDTERYRPVAQLA
                     SRGQGQVYSARFVTSSQVITACGDGAARLWDGETGQLRRTYRGGSRFLIDATLSGDGS
                     MLIGGDGDGQLRFWEAASGRPLWTMQAHRSHLIGVHVEGTDIVTRGFSGDIARWSLPR
                     PEQVIHACDHNERCAIVPE"
BASE COUNT          897 a         1704 c         1725 g          767 t
ORIGIN      
        1 aagtacgacc acgccgccga ctcgacgccc tcgacgttgc cgccgtacgg cgtgcgcgac
       61 aggtactcgg cgaggatctc gcgcttggac agccggaggt cgagctgcac ggcgcggaac
      121 atgtcgacca gcttggcgcg cagcgtgcgc ggccgcggct cgagcagccg cgccagctgc
      181 atggtcaggg tcgagccgcc cgacacccgg cgggcgtggc gcgcgtcgct cgccgcggcg
      241 cggacgatcg cgatcggatc gacgccgtcg tggcgccaga accgcttgtc ctcgagcgcg
      301 atcagcgcgg cgaccagcgc cgggtcgacg tcgtcgagcg cgatcggcag ccgccacttg
      361 tcgtcgcgcg ccaggaacac gtgggccggc gtgccgtccc ggtactcgat cgcagcggac
      421 cactcgccgc gccggtccgg aagcggaacg gcgagcgcga gcgcgagcac ggcgagcagc
      481 gccgcgttga ccgcggtgat cgcgacgagc gcgccgcgga cgagccgccg acgccggcca
      541 gtccgcagga tcaccacgac gtctggccga tcacatccgc tcgctgcgct cgcggtggca
      601 agcggcgcga ggcagatcga ggctcacccc cgggagggga gatcgaaaac ccgttggtcg
      661 tcgacgcact gcaacctcgc atgcacgtgc gcccagacgg atcgcgcccg atcccaccac
      721 gccccgctgg atcccgtcgg gtgtggccaa gaccctggca acgagccagc cctcaggcgc
      781 gggcatcgaa gctggatgac ttcggcgttg agccatcgcg agcgcctact ctagcgcgcc
      841 ggccagctca aatacgccat cgacccgagc gcggcggccg accaggggga atcgaacccc
      901 atgagagcca gctgaccccc taccttggta tcgcggcttt gcaacggttc tcgggggttg
      961 cgtcgcccac cgcgctggct acgtccggcc tgatccgcct gtgtcccgtt cgtcgtggca
     1021 catctgtgcc acaactcgcg actgcggatc ctcgtcggga tggctttgct tcttggggtc
     1081 gaacttttcc gtgcactcgg tccgctgatc gctgattgtt gatggcggtc cggacccgtc
     1141 gaaatctgac gcgaggccct cgctcgcgat gaacggcaac cagaatccgt cgcgtttcga
     1201 cgcgactcct atgcctcgcg tcgattttcg accgatcccg ctgtcgcaat cgccggtcgc
     1261 cgcgggcgtc aaatttcgac ggattctgac agccgtccgc gcgcgcgacc gcccccgcgg
     1321 cgagcgcgaa ggccgcgtag tcgatcttcc tgggcatggt cgaacctgtg gcatctcgcg
     1381 taccttcccc gctgggaacg aaccgatcgg ccggagcgtt atgctcgcgc gatgatgaag
     1441 agcctggtgt tcggcgtact ggtgggcacc gctgcggcgg cgcacgcgga tgcaaagccg
     1501 accgatctcc ccgcggagct gaaggcgatc gcggcgaagt atccgccgcc gaaggaccag
     1561 cgctggcagc ccgccgcggg cccggggtgc acggcgccca ccgcggccga gcgcgcggcg
     1621 gccatgaagc gcgcgttcgc gtggatcgat cagcagcacc ccgacgaggt cgggtcgaac
     1681 caggtcacga acaacacccc cgaggtcgag atgaacgtcg gctgcaagga cgcggcgggc
     1741 gtgctcgtgc tcgacgtgag ccaggaccgc gacctcaaga agaagaagaa ggaggacggg
     1801 ttcagcgagc ggcggcgcaa ctacctgctg aagctctcgg cggccgcgat cgacgtgatc
     1861 gcggaggaca cgagcgactc gacgcaggcg tggtcggagt gggcggatga aggaaggatc
     1921 tcgctgctcg cccagctcga cgtcgacggc gacggcgcgc tcgacatcgt gtactcggat
     1981 caccagcacg agggcggcgc gaagagcacc ttcgacagcg tgcacgtgcg gttcgcgacc
     2041 ggaaagaccg gcgaggcggg ccgcgtcacg aacctcgtgg atcggaaggt cgtgaacaag
     2101 cagctcgtga tcgcgggcga gttacgcgaa gacgcgcgcg tttacgcgtg cctcggagcg
     2161 gacctgcact tctcgccgtg cgccgcgtcg aaggcgctgc aggccgaggc cgacaagcgc
     2221 gccatcatcg accggttcag ccaggcggcg ccgaacaacc tgccggatcg cgagcagctc
     2281 gcgatggacc tcggcgcgct cggcacgcgt gcgcagcggc gggcccagct cgtcgcggcc
     2341 gcgcccgaga cggatccggg gacgcacgtg cagcgcaagg cgatcgcgtt cctcgtcaac
     2401 gcggggctgc tcgagccggc gccgatgccg gatatcttcg accagacgca ccccgaggcg
     2461 cagacgtacc tcgacgggct cgccgcgaag ctcggcgaca ccacgtgcgc gacgtcgccg
     2521 ctgagcgccg aggacaccgc gaaggcgaac gcgtggatca agaagcagga cgagaaggcg
     2581 agcgagatca agatcgagcc ggcgtcctgc ggtccctaca cgtgggtcag ctggtggccg
     2641 ccgaacaaca ccgacaacaa gcggcgtgaa gcgctgctcg gccgcgacgg aacgcggatc
     2701 atcggcatga cgtacgagcc gcagccgtac aacccgcaga taccgggcga cgagggcttc
     2761 tcgttcacgc agcagtggtt cagccacgac ggcgcgatcg tcggcatcgt catcggcagc
     2821 ggcaacctgt tcgtgatctc gaacggcaag gccgtcgcgt cgacgaaggg caacaacctc
     2881 gggctgtatc acgcggacga ccgattcagc gagtcgagcg tcgacgtgtt tgtcgacggt
     2941 ggcacgctgt ggcacgcgac gccgacgggc cgcgagcggc tcgacatggc gctggtcaag
     3001 gatcacgagg cgcggcgcgc ggccatcgcg ctgctcgtcg cagggccggc cgggaacgcg
     3061 gcgcagtacg cggcggcgct cgagctgctc ggcgcggaca aggcgctcgt cgccgaggcg
     3121 aagaagttgt agctggcccg gatcattcgc gagggcggtc gccgcaggcg accaagcgac
     3181 ggtcggccgg atcggcgggc ggcggggcgg ccggcagcgc ccgcaggcgc ctggaatcta
     3241 cgcgaggcca cgccgcctcg ctcgcgatga acggcaacca gaatccgtca catttcgacg
     3301 cgactctatg cctcgcgtcg attttcgacg gatcccgctg ccgcaatcgc cggtcgacgc
     3361 gggcgtcaaa tttcgacgga ttctgactgc caattggtcg acctcacacc gaccgcgggc
     3421 gccggacgag gcggcggggg cgacgtcgat cgcgagcaga acctcggtgt ggtcgccgca
     3481 gccgccgcgg tcaacgccag cgcttgcgga tagattcctt catttgggca caggtacggc
     3541 gtcctgggct acgcagttga tgcccgactg ggtaccgttc gtcggcctgg cagcgtcatc
     3601 cccgcccctg gcgcgcttga ggtcgatgtt gtcgagcgtt cggatggttt cgctatggag
     3661 tcccagcttg cggttatttt tcttcattcc ggtacgatag cgcaccgttc gttgtgatcg
     3721 cacgcatgga tcacttgctc gggtctgggc aaggaccacc gtgcaatatc gcccgaaaat
     3781 ccccgcgtta cgatgtcggt gccttcgaca tggaccccga tgagatggga gcgatgtgcc
     3841 tgcatcgtcc acagcggacg tcccgaagca gcttcccaga atcgcagctg accatcgcca
     3901 tccccgccga tgagcatgga gccatcgccg gacagcgtgg cgtcgatgag aaagcgcgag
     3961 ccgccgcgat aggtccgacg gagctgtccg gtttccccat cccagagccg ggcagcgccg
     4021 tcgccgcatg cggtgatgac ctggctactt gtgacgaagc gcgccgagta gacctgaccc
     4081 tgtccgcggg aggcgagctg agccacgggg cgatagcgct ccgtgtccca cagctccggc
     4141 gcagcgacct tgcccgggaa gataggaagt gttaccaggc gatgcccgtc cgacgacatc
     4201 cgtaacgtgc caacgcgtgt ttgagtctcg aagtcggcaa gcgtggctcc accgagatca
     4261 tagatccgca gatgccgtcc agcatccacc acgacgattc gaccgtcgga gagaaacccc
     4321 gccgtatcga tgccagctgg cgcggtcggc agcatcagca gcgtgccgtt atctcgcgtt
     4381 acgatgacag agccgtcgat ggctccgctg acgatgtctc gccctgctgc ggcgaaggcc
     4441 accgcgctaa cagatgcacc atgcttgact gtgcgcagca gccgagcggt aggtagctca
     4501 taaacttcga catcgttgcc acgtgcgatc gccgcgcagt ctccagcggc tgaaacggcc
     4561 ggatatatcg gtgcgaaacc atcaccgatg ggggtcacga acggcagctc agcgacgagg
     4621 cgattctgtg ccgtgtccca aatgcgggtc ggttcatcat tgcaacggat ggccagaaag
     4681 cgccggtctg gctcgaggct ggtgatgata ccgcagtcct cgtcattgaa cggcgagccc
     4741 caacgtcgat acatcgcggt cgcgtcccag atccgggctg taccatccca ggaagcacca
     4801 acgacacgcc gcgagctcgg gtcgaaatgc gcagcccgca ctttcgcggt aggcccgtca
     4861 agctccgtga ccggcatccc cgatgtggcc tcgattacgg caatggagcc gcttgcgcta
     4921 gacgccgcaa ttaacgagga ggtttgatcg aactcgatgg agaggacatc gctccgtagc
     4981 tcgttgctcc ggctccgcaa cgtcccagac gtcgcatccc agacctgcac tgtaccgtca
     5041 tggctccccg tgacggcgag ccgaccatcg ggcgagaacg ctaccgcatc gat
//
LOCUS       DASVOR010000509         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_472301,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000509 DASVOR010000000
VERSION     DASVOR010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472301"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VF516_10190"
     CDS             complement(<1..463)
                     /locus_tag="VF516_10190"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEX8108086.1"
                     /translation="MARTRKPFARRLADTLPRRRRSPELARREILDAAERVFARYQPD
                     QVGLKEVAREAGVSHALITHYFGTYAGLLEATLQRRIRAVREAAIARLREPEALSRPA
                     DLLELLFRTLQDPVHLRLMRWMLASERPAASHAFALQDRGLAVLAEQVARAV"
     gene            551..1354
                     /locus_tag="VF516_10195"
     CDS             551..1354
                     /locus_tag="VF516_10195"
                     /inference="COORDINATES: protein motif:HMM:NF016879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Coq4 family protein"
                     /protein_id="HEX8108087.1"
                     /translation="MQTVTSSSDHPTATPTPGPSTQGAPGATAGTPAGMPIDLDSLTS
                     LQRWKLGLGLLRKVLDNPEDTEQVLAFLGVINSGRSTQARVARFFADPEGEVLYAEHR
                     AIDSRTVDLDRLAALPEGTLGHAYAKFLRSHGITPEIFDGAPPGITNPRRSYVVQRMR
                     QTHDLWHVVTGCETDPAGEVALQAFTYSQTGSPGSAFLAVFGALRRVREQRDVGVLRD
                     VATLYRTGKRAGRRLPTFAWEDHWETPLAEVRAMLGLPIEPPARAATMS"
     gene            complement(1409..2869)
                     /locus_tag="VF516_10200"
     CDS             complement(1409..2869)
                     /locus_tag="VF516_10200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108088.1"
                     /translation="MTERTGRPSPIDARTAAVRPGDPAQRYRVIEKLESGGMAEVFRA
                     ESTGLQGFRKQVAIKRVLPHLGRKSKFISMFLDEARLSAQLSHSNCVQVFDIGVGDDA
                     YFIVMEYVEGANLKSIATSMRNQGRTFPIPAAAYIAHEICKGLSYAHELTDRSGMALH
                     LVHRDVSPPNVLVTKYGEVKIVDFGLAKASSQLEESEPGIIKGKFAYLSPEAALGQEV
                     DRRADIFAVGIILWELLASQRLFAGDTDVQILHKVQAAGVPPISQINRSVPPDLERII
                     HRALARDRHERYSTARELGQELWRFLFRFAQPVSGYDIATIVQDTMREKENARLAQGS
                     FIHKLIEEALLQFTSLKGDSTTSSGLGAAAAPLNADLFVDPESPAGPPAVPGRSGDGP
                     EQDNLAALEEDLENDLEDHDDAAMRSGLGAPRPATSRAPGDGVPAPRSERDPASGSDL
                     APGASRRGRRVALGIALLVLVTAAALAWCTGMIPYR"
BASE COUNT          430 a         1091 c         1058 g          420 t
ORIGIN      
        1 tgaccgcgcg cgcgacctgc tcggcgagca ccgcgaggcc gcggtcctgg agcgcgaacg
       61 cgtggctcgc cgccggccgc tcgctggcga gcatccaccg catcagccgg aggtgcacgg
      121 gatcctgcag cgtgcggaac agcagctcga gcaggtcggc cggtcgcgac agcgcctcgg
      181 gctcgcgcag ccgggcgatc gccgcctcgc gcaccgcgcg gatccggcgc tgcagcgtgg
      241 cctcgagcag cccggcgtag gtcccgaagt agtgcgtgat caacgcgtgg ctcacgccgg
      301 cctcgcgcgc gacctccttg agcccgacct gatcgggctg gtagcgggcg aacacgcgct
      361 cggccgcgtc gaggatctcg cggcgcgcca gctccggact gcgccgccgc cgcggcaatg
      421 tgtccgcgag ccggcgggcg aaaggcttgc gagtccgcgc cattaggcga acctaccacg
      481 attctatctt caccttgaca acaaccggat cgctattatc attgtgacaa taggtgatca
      541 ggagctggcg atgcagaccg tgacctcttc ttccgaccat ccgaccgcga ccccgacccc
      601 tggcccgtcg acccagggcg ccccgggcgc gacggcgggc acgcccgcgg gcatgcccat
      661 cgacctcgat tcgctgacca gcctgcagcg ctggaagctc gggctcggcc tgctccgcaa
      721 ggtcctcgac aaccccgagg acaccgagca ggtgctcgcg ttcctcggcg tgatcaacag
      781 cggccgctcg acccaggcgc gggtcgcgcg gttcttcgcc gatcccgagg gcgaggtgct
      841 gtacgccgag caccgcgcga tcgactcgcg caccgtcgac ctcgacaggc tcgccgcgct
      901 gcccgagggc acgctcggcc acgcctacgc gaagttcctg cgctcgcacg gcatcacgcc
      961 cgagatcttc gacggcgccc ccccgggcat caccaacccg cggcggtcgt acgtggtcca
     1021 gcggatgcgg cagacccacg acctgtggca cgtcgtcacc ggctgcgaga ccgatccggc
     1081 cggcgaggtc gcgctccagg cgttcacgta ctcgcagacc ggctcgcccg gcagcgcgtt
     1141 cctcgcggtg ttcggcgcgc tgcgccgggt ccgcgagcag cgcgatgtcg gcgtcctgcg
     1201 cgacgtggcg acgctgtacc gcaccggcaa gcgcgccggt cgccggctgc cgacgttcgc
     1261 gtgggaggac cactgggaga ccccgctggc cgaggtccgc gcgatgctcg gcctgccgat
     1321 cgagccgccg gcgcgcgccg cgacgatgag ctgacccggt tgcgcccggc gaagctctgc
     1381 cgggtcggtc gaacggccga ccgagccgtc agcgatacgg gatcatcccg gtacaccacg
     1441 ccagcgccgc ggccgtgacc aggacgagca gggcgatccc gagcgccacg cggcgcccgc
     1501 ggcggctcgc tcccggagcc aggtccgagc cggacgcggg atcgcgctcg gagcgcggcg
     1561 ccgggacgcc gtccccgggc gcgcgggagg tcgccggccg cggcgccccg agcccggacc
     1621 gcatcgccgc atcgtcgtga tcctcgagat cattctcgag atcctcctcg agagcggcga
     1681 ggttgtcctg ctcgggcccg tccccggacc ggcccggcac ggcgggcgga ccggccgggc
     1741 tctcgggatc cacgaacaga tccgcgttga ggggcgccgc cgccgccccc aggcccgagc
     1801 tcgtcgtgct gtcgcccttg agcgaggtga actggagcag cgcctcctcg atcagcttgt
     1861 ggatgaacga cccctgcgcc aggcgagcgt tctccttctc gcgcatcgtg tcctgcacga
     1921 tggtcgcgat gtcgtacccg ctgaccggct gcgcgaagcg gaacaggaag cgccacagct
     1981 cctgcccgag ctcgcgggcc gtcgagtagc gctcgtgccg gtcccgggcg agcgcgcggt
     2041 ggatgatgcg ctcgagatcg ggcgggacgc tgcggttgat ctgcgagatc ggcgggaccc
     2101 ccgcggcctg gaccttgtgg aggatctgga catcggtgtc gccagcgaac aggcgctgcg
     2161 atgccagcag ctcccacagg atgatcccga ccgcaaagat gtcggcgcgg cgatcgacct
     2221 cctgtcccag cgcggcctcg ggcgacaggt aggcgaactt tcccttgatg atgcccggct
     2281 cgctctcctc gagctgggag ctggccttgg cgagcccgaa gtcgacgatc ttcacctccc
     2341 cgtacttcgt gacgagcacg ttcgggggag acacgtcgcg atgcaccagg tggagcgcca
     2401 tgccgctgcg atcggtcagc tcgtgggcgt agctgaggcc cttgcagatc tcgtgagcga
     2461 tgtacgccgc ggccgggatc gggaaggtcc ggccctggtt ccgcatcgac gtggcgatcg
     2521 acttgaggtt cgcgccctcg acgtactcca tgacgatgaa gtaggcgtcg tcgcccaccc
     2581 cgatgtcgaa cacctggacg cagttggaat gcgagagctg agccgacagc cgcgcctcgt
     2641 ccaggaacat cgagatgaac ttcgacttcc ggcccaggtg cggcaggacg cgcttgatcg
     2701 cgacctgctt gcggaacccc tggagcccgg tgctctcggc gcgaaacacc tcggccatgc
     2761 cgccggattc gagcttctcg atgacgcggt agcgctgcgc gggatcgccg ggccgtactg
     2821 cggcggtccg ggcatcgatg ggcgagggcc gccccgtgcg ctcggtcatc gagctaaccg
     2881 atccggccgg cggcggcgcg ggcgagccac agcgccgcga ggaacagcgc gatgcacgcg
     2941 gccgcgccgg ggtgcccccc ggagtgtgcc ggtgagcgcg gcgcacccgg ccgatcggc
//
LOCUS       DASVOR010000510         1980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_355402,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000510 DASVOR010000000
VERSION     DASVOR010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_355402"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1472)
                     /locus_tag="VF516_10205"
     CDS             complement(162..1472)
                     /locus_tag="VF516_10205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108089.1"
                     /translation="MIQRAWFALCFAVLIVACGGKHGNSPDASNPCGVFGTPCKAGGD
                     CCSGMCDSTGSCTVNGTMCTAAGSSCSANTDCCSLSCVGDVCQGKQCIADGAACSQDG
                     ECCGGSCQNNKCTPLSTACKTAGNPCGGNAECCSSFCNANHTCGAASWCAQDGDACGH
                     DAECCGGICEKGSTGTLGLCTHPMPGSTNCSAGVDGTVCTDCGSCCSRLCEVYAPTGV
                     KVCQPAEGCRVDGDLCRKDSDCCGAAGTGLPGDGHVTCLKQNPNDPTGICRNPNACNP
                     EGDVCHYQNYATCGNSSARNDCCGGLGNSGVCQLDAVGVPRCYGLGACRMSGETCAFA
                     GDCCNGLPCVPDASGALHCGTTACVAMGGMCTASADCCTGAPCVFVPGQTYGTCGGGG
                     TCALDGQSCKQTGDCCNMDCNVTGSNPPVTCNGGTNCTCFTILF"
     gene            complement(1694..>1980)
                     /locus_tag="VF516_10210"
     CDS             complement(1694..>1980)
                     /locus_tag="VF516_10210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108090.1"
                     /translation="IDRDGAEYDRLAALEPTSPAAALKGYLALARGTSRWADPALFAA
                     ARLAADRHDPRAETLLGVYLQRFPGGANAADARQLLARLQADKRDKRDKP"
BASE COUNT          312 a          688 c          662 g          318 t
ORIGIN      
        1 gggtccgaca ccgcgctgct ctcgatcgcg atcgagctcg cgcaggcccc ggccgagacc
       61 gtgggcccgc cccccgccga cccggtgtgc gaccgctcga actgatcgct acaggatcgc
      121 cgaaccagct cggacactcg cgatcagccg gcgaacacga gtcagaacag gatcgtgaag
      181 caggtgcagt tggtcccccc gttgcaggtc accggcgggt tcgagcccgt gacattgcag
      241 tccatgttgc agcagtcgcc ggtctgcttg caggactgac catccagggc acacgtgcca
      301 ccgccgccgc aggtgccgta ggtctggccg ggtacgaaca cgcagggtgc gccggtgcag
      361 cagtccgcgc tcgcggtgca cataccaccc atcgcgacgc atgcggtcgt tccgcagtgg
      421 agggcgccgc ttgcgtcggg cacgcacggc aagccgttgc agcagtcgcc ggcgaacgcg
      481 caggtctcgc cgctcatgcg gcacgcgccc aggccgtagc agcgcggcac gccgaccgcg
      541 tcgagctgac acacgccgga gttgccgagg ccaccgcagc agtcgttgcg cgccgacgag
      601 ttgccgcacg tcgcgtagtt ctggtaatgg cagacgtcgc cttcggggtt gcatgcgttc
      661 gggttgcggc agatgccggt ggggtcgttc gggttctgct tgaggcaggt cacgtggcca
      721 tcgcccggca acccggtgcc ggccgcgccg cagcagtcgc tgtccttgcg gcagagatcg
      781 ccatcgacgc gacacccctc ggccggctgg cagaccttca caccggtcgg cgcgtagacc
      841 tcgcacaggc ggctgcagca gctgccgcag tcggtgcata ccgtaccatc gacgccggcg
      901 ctgcagttgg tgctgccggg catcgggtgc gtgcacaggc cgagcgtgcc ggtcgagccc
      961 ttctcgcaga tgccgccgca gcactcggca tcgtgcccgc aggcgtcgcc atcctgggcg
     1021 caccacgacg ccgcgccgca cgtatggttc gcgttacaga acgacgagca acactcggca
     1081 ttaccgccgc acgggttgcc ggcggtcttg catgcggtgc tgagcggtgt gcacttgttg
     1141 ttctgacacg acccgccgca gcactcgccg tcctgcgagc acgccgcgcc gtcggcgatg
     1201 cactgcttgc cctgacacac gtcgccgacg cagctcagcg agcagcagtc ggtgttcgcg
     1261 ctgcacgacg agcccgcggc cgtgcacatt gttccattga cggtacagct gccggtcgag
     1321 tcgcacatcc ccgagcagca gtcaccgccc gccttgcacg gcgtgccgaa cacgccgcat
     1381 ggattgctcg catccggtga attaccgtgc ttgcctccac aagccacgat gagcaccgcg
     1441 aagcacaacg caaaccaggc tctttggatc attggtatat cttcctcgat ccgtgaaaat
     1501 gagcaagcaa ctgctcatga tctgtgcgaa cacgcctttg tgatttccca ctcagtgtac
     1561 cgttcgtaca ctggactgta cgaaacggta catgcggatc gacacagctg ctgtacggtt
     1621 cggtgcaccc gtggcgcacc gacggcgctg accgcttccg aggagccgat ggccgagccg
     1681 tgcgaagacg gggctagggc ttgtcgcgct tgtcgcgctt gtcggcctgg agccgggcga
     1741 gcagctgccg ggcatcggcg gcgttcgctc cgcccgggaa gcgctggaga tagacgccga
     1801 gcaaggtctc ggcgcgcgga tcatggcgat cggccgcgag ccgcgcggcg gcgaacagcg
     1861 ccggatccgc ccaccggctg gtcccgcgcg cgagcgccag gtatcctttg agcgccgcgg
     1921 cgggcgacgt cggctcgagg gccgccagcc ggtcgtactc ggcgccgtcg cgatcgatct
//
LOCUS       DASVOR010000511         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_472328,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000511 DASVOR010000000
VERSION     DASVOR010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472328"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VF516_10215"
     CDS             complement(<1..267)
                     /locus_tag="VF516_10215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108091.1"
                     /translation="MPESDDTNNTIAVPVTIGQAGLVVSSLTGVPAQSSSQQLLPVSW
                     TVKNQATTGAASGSFVPCCAQTDHGSYQYNNIWFDRFFLSTDAAL"
     gene            complement(304..1281)
                     /locus_tag="VF516_10220"
     CDS             complement(304..1281)
                     /locus_tag="VF516_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX8108092.1"
                     /translation="MRRSRNLIPRFTLRHLPAGASAVAVLAALSILGCQEPPEEASTA
                     AASTGLLANGAACTASDQCSSGVCSGGVCVASPDLVVASITGVPAQVSAQQLVAASWT
                     VRNQGAASAHGSTVPCCSQTDHGSYQYNNLWFDRFFLSTDAVLSADDVFIGEYARPDN
                     FTLAAGASYTASASIHVPSVAAGSYYLILVTDGYGNRVPESDDTNNTLAVPVTVGQAD
                     LVISALTGVPAQASSQQLVPVSWTVKNQATTGAASGSFVPCCAQTDHGSYQYNNIWFD
                     RFFLSTDAALSADDVFISEYARPDNFTLAAGASYTASASIHVPPVAAGS"
     gene            complement(1310..>1875)
                     /locus_tag="VF516_10225"
     CDS             complement(1310..>1875)
                     /locus_tag="VF516_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108093.1"
                     /translation="PPDNTAKPLDPSKYSFELTPPGTKATGFQFTAKDIEGGPFHLAV
                     RPSSKPDAYDVEVAVGSAPPSTRTTELCAGGGGIAPALAHSNGLLSAAAALSAELTSK
                     LSQFEAPDRLMRIVIGTLGRTVDMQRLTRAVRAQPCPATASLEFVCAHPRCRVKLQGA
                     ERNLNQGDYLSLASGASVEFMTGEQTN"
BASE COUNT          297 a          601 c          674 g          303 t
ORIGIN      
        1 gagcgccgcg tcggtcgaca ggaagaaccg atcgaaccag atattgttgt actggtacga
       61 tccgtgatcg gtctgggcac agcagggcac gaaggagccg ctggccgcgc cggtcgtggc
      121 ctggttcttc accgtccacg acaccgggag gagctgctgc gaggacgact gtgctggcac
      181 gccggtgagc gaggagacca ccaggcccgc ctgcccgatg gtgaccggga ccgcgatcgt
      241 gttgttggtg tcatcgctct ccggcacgcg attcccgtag ccgtcggtga cgaggatcag
      301 gtactaggag ccggcggcca ccggggggac atggatgctc gcgctcgcgg tatagctggc
      361 gccggcggcc agggtgaagt tgtcgggccg ggcgtactcg gagatgaaga cgtcgtcggc
      421 gctgagcgcc gcgtcggtgg acaggaagaa tcgatcgaac cagatattgt tgtattgata
      481 cgacccatga tcggtctggg cacagcaggg cacgaaggag ccgctggccg cgccggtcgt
      541 ggcctggttc ttcaccgtcc atgacaccgg gacgagctgt tgcgaggacg cctgtgcagg
      601 cacgccggtg agcgcggaga tcaccaggtc cgcctgcccg acggtgaccg ggaccgccaa
      661 cgtgttgttg gtgtcatcgc tctccggcac ccgattcccg tagccgtcgg tgacgaggat
      721 caggtaatag gagccggcgg ccaccgacgg gacgtgaatg cttgcgctcg cggtatagct
      781 ggcgccggcg gccagggtga agttgtcggg ccgggcgtac tcgccgatga agacgtcgtc
      841 ggcgctgagg acggcgtcgg tcgacaggaa gaaccgatcg aaccagaggt tgttgtactg
      901 gtacgaccca tgatcggtct gcgagcaaca cggcaccgtc gagccgtgcg ccgaggccgc
      961 cccctgattg cgcacggtcc acgacgcggc cacgagctgc tgggcggaca cctgcgccgg
     1021 cacgccggtg atcgacgcga cgaccaggtc gggcgacgcg acgcacaccc ctcccgagca
     1081 caccccgctg ctgcattgat cgctggcggt gcaggcggca ccgttggcga gcagccccgt
     1141 gctggcggcg gccgtcgaag cctcctccgg cggctcctgg catccgagga tcgacagcgc
     1201 agcgaggacg gcgaccgcgc tcgcccccgc cgggagatgt cgcagcgtga atcgcgggat
     1261 gaggttgcgg ctccgacgca tagggtgccc ctcttggcta gccagctcgt taattcgttt
     1321 gttcgcccgt catgaactcg accgacgcac cactcgccaa gctcaggtag tctccctgat
     1381 tcaagttgcg ctccgcgcct tgcagcttga cccggcagcg cggatgggcg cagacgaact
     1441 ccaggctggc ggtcgccggg cagggctgcg cgcggaccgc ccgggtcagg cgctgcatgt
     1501 ccaccgtgcg gcccagggtc ccgatgacga tacgcatcag ccggtccggc gcctcgaact
     1561 gcgatagctt gctcgtcagc tccgccgaca gcgccgcggc cgcggagagc aggccgttcg
     1621 aatgggcgag cgccggcgcg atcccgcctc cgccggcgca cagctcggtg gtgcgcgtgc
     1681 tcggcggcgc ggatcctacc gccacctcga cgtcgtaggc gtccggtttg ctggagggcc
     1741 tgaccgcgag atggaacggc ccgccttcga tgtcctttgc ggtgaactgg aaaccggtcg
     1801 ccttcgttcc ggggggcgtg agctcgaagc tgtacttcga gggatcgagc ggcttggccg
     1861 tattgtcggg cggca
//
LOCUS       DASVOR010000512         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_472363,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000512 DASVOR010000000
VERSION     DASVOR010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472363"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /gene="nusB"
                     /locus_tag="VF516_10230"
     CDS             <1..403
                     /gene="nusB"
                     /locus_tag="VF516_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEX8108094.1"
                     /translation="LQLLYARDGDPASDVADAAAAWAEAFDIELDASAQAFARELVAA
                     ASQHAVQIDALIGAASKNWRIDRMSRVDRNILRLGACELIAFPDVPVKVVINEAVELA
                     KRFGTAESSAFVNGVLDRIATAVGRRPEDED"
     gene            406..900
                     /locus_tag="VF516_10235"
     CDS             406..900
                     /locus_tag="VF516_10235"
                     /inference="COORDINATES: protein motif:HMM:NF014808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8108095.1"
                     /translation="MALSVLPLDLARWDEAARDTLVLPVFKDDRPLRGAAGLADWRLC
                     GRLSRLVKSSRATAEAGETLLLPPGRRLRFSRIVWFGLGDARGYTDERFRKDLTRIRE
                     VVIGVGVADWALQAPGRASGLIGARRAVEIMLEDAVLVEQPVTLLEDPAGQKDIAELL
                     RQSR"
     gene            complement(931..>1524)
                     /locus_tag="VF516_10240"
     CDS             complement(931..>1524)
                     /locus_tag="VF516_10240"
                     /inference="COORDINATES: protein motif:HMM:NF017833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEX8108096.1"
                     /translation="LLQELGAHHGDAPPPPREASLSVGALVDKAAESGFGFVIGVLSL
                     IAIPFVGLSTPFGLAIALLGAQLMIGLRHPWLPARARRRALSMAMLDRVLGLLVRRTR
                     WLTRLSRRRWEPLIQPRLVGLGVVLLALGLALPLPIPGSNLVFLIPLFTYSIGVLERD
                     GLWIVLGHLGTLINMALLVVFGATVVEVLQRIWHWIA"
BASE COUNT          221 a          575 c          541 g          187 t
ORIGIN      
        1 gctccagctc ctctatgcac gcgatggcga tccggcctcc gacgtcgccg acgcggcggc
       61 ggcctgggcc gaggcgttcg acatcgagct cgatgcgagc gcgcaggcgt tcgcgcgcga
      121 gctggtcgcc gcggcgtccc agcacgccgt gcagatcgac gcgctgatcg gcgcggcgtc
      181 caagaactgg cggatcgatc gcatgtcgcg cgtcgatcgc aacatcctgc ggcttggcgc
      241 gtgcgagctg atcgcgttcc ccgacgtgcc ggtcaaggtc gtgatcaacg aggccgtcga
      301 gctcgccaag cggttcggca ccgccgagtc gtcggcgttc gtcaacggcg tgctcgaccg
      361 gatcgcgacc gcggtcggcc gccgccccga ggacgaggac tgaccgtggc gctctcggtc
      421 ctcccgctcg atctcgcccg ctgggacgag gccgcgcgcg acaccctcgt gctcccggtg
      481 ttcaaggacg atcgcccgct gcgcggcgcc gccggcctcg ccgactggcg gctgtgcggc
      541 cggctgtcgc gcctggtcaa gtccagccgg gccaccgccg aggccggcga gaccctgctc
      601 ctgccgccgg gccggcgcct ccggttctcc cgcatcgtgt ggttcggcct cggcgacgcc
      661 cggggctaca ccgacgagcg gttccgcaag gacctgaccc ggatccgcga ggtcgtgatc
      721 ggcgtcggcg tcgccgactg ggcgctgcag gcgccgggcc gcgcctcggg cctgatcggc
      781 gcgcgccgcg ccgtcgagat catgctcgag gacgccgtgc tggtcgagca accggtcacc
      841 ctgctcgagg atccggcggg ccagaaggac atcgccgagc tgctgcggca atctcggtga
      901 gcggcgcggt gagccgccgc gccgccgccg tcaggcgatc cagtgccaga tccgctgcag
      961 cacctccacc accgtcgcgc cgaacaccac cagcagcgcc atgttgatca gcgtcccgag
     1021 gtggccgagc acgatccata gcccgtcgcg ctcgagcacg ccgatcgagt aggtgaacag
     1081 cgggatgaga aacaccaggt tcgaccccgg gatcggtagc ggcagcgcga gcccgagcgc
     1141 gagcagcacg acccccagcc cgaccagccg gggctggatc agcggctccc agcggcggcg
     1201 cgacagccgg gtcagccagc gcgtgcgccg gacgagcagc ccgagcacgc ggtcgagcat
     1261 cgccatcgac agcgcgcgcc gccgcgcccg ggccggcagc cacggatggc gcagcccgat
     1321 catcagctgg gcgccgagca gcgcgatcgc cagcccgaac ggcgtcgaca gcccgacgaa
     1381 cgggatcgcg atcagcgaca gcacgccgat cacgaagcca aacccgctct cggccgcctt
     1441 gtcgacgagc gcgccgacgg acaggctcgc ttcgcgcggc ggcggcggcg catcaccgtg
     1501 gtgcgcaccg agctcctgca gcag
//
LOCUS       DASVOR010000513         5555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_92780,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000513 DASVOR010000000
VERSION     DASVOR010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92780"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VF516_10245"
     CDS             complement(<1..869)
                     /locus_tag="VF516_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108097.1"
                     /translation="MHKAVVVVIALALVGAASRRAAAYPQYQLSRDQTCTGCHLSPAG
                     GGLLTENGYSVAETQALLGDSSAFMYGLFKLPGWLALGGDLRGSTGYFKAPDGSFALI
                     PMQADVYASATAKGFTLHVTVGGRPAQWVGVNNTPAIVDRFWSREHYVMWQQDENGHD
                     GLFVRVGRFMPVFGLRFAEHPIYTRRFGGTPLYGETYGAAVEYVKDTWEAHVTGFLED
                     PLIDTVVHDSGGAGYAEVRLNPRLQLGGELMVTSSDGLARVRGGATGKLYIPSADTLI
                     QAELQVVHQQVHGP"
     gene            complement(854..1435)
                     /locus_tag="VF516_10250"
     CDS             complement(854..1435)
                     /locus_tag="VF516_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108098.1"
                     /translation="MKQLVLIGALLGSAAGACGATDDRPRNLEYITQTILAPTCAAAE
                     CHSAFKREVGDEFDTVDATRRTIVNNGLVNPGNPGSSVLYTSITIGAPSILSPGSTVR
                     MPFDAPMPDADVALIAAWIQDGAPGAQCLANDQGNGCSVTGTGAGRVYHVVQCVDGNI
                     GAVVMDCPLADPASNKQQSVCSTTTGNGQCTKQ"
     gene            complement(1494..2972)
                     /locus_tag="VF516_10255"
     CDS             complement(1494..2972)
                     /locus_tag="VF516_10255"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8108099.1"
                     /translation="MSTVEQQSSQLRQELGLTDLVLTQILYVVGLSWVGTAAKLGTSQ
                     IPFWLGAMAMFYVPQAIVVVHLSRRFPLEGGLYQWSKLGLSEGMAFLVGWNLWIYAIV
                     LLGAFGLQFANSIIYAIGPAAQSLSGNHWFMGAVSGGLIGALTVLSILGLRTSKWVHN
                     AGAVLLLLAFGLLLALPAINVARGTLREYHPLAVTAPALTVFNLNIFGKLAVGALSGF
                     EYVAVLAGETRNAGRNIGRSVMIAAPVIALMFILGTSAIQALTPPDQVDLVGPIPQAF
                     RNGLPGSGAVAILIPLAVFAVSARSIGNSSLLFTATSRMPMVAGWDRLIPAWFSRLHA
                     RFRTPVNSVLFIGGAGLVFAILGMLGVGEQEAFQLLDNAAGILYGLTYLVLFAVPLVG
                     LARTGRRAPPWIRIAAAVGFATTSLFVVLSVLPIVEVESRLAFVAKIGGTVVAVNLIG
                     AALYLVRRPRGAAAVIQIADGADGADGAGPVPHAERKPANDP"
     gene            3318..3764
                     /locus_tag="VF516_10260"
     CDS             3318..3764
                     /locus_tag="VF516_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108100.1"
                     /translation="MGSTKHSFVHSIQPSALPPPYQLAIEDAEFQLTLLEDGAPRSHD
                     PGCAVVPPTWFSVALTAPTRKRYLYLVQKETDTGLIVVIMLFDRSATGSDAAGLRLPS
                     SGAWLRAVVDGTVYVLASDLVLTRKAITAWIGGREPPTTPPQPPYT"
     gene            3830..5473
                     /locus_tag="VF516_10265"
     CDS             3830..5473
                     /locus_tag="VF516_10265"
                     /inference="COORDINATES: protein motif:HMM:NF023500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3556 domain-containing protein"
                     /protein_id="HEX8108101.1"
                     /translation="MSLLAPTPAPYDPLDWVQRPFAERARMVCEAWAIQGYGTPPLIY
                     AVHVVKIALYVGAWLMFCSTSPGLGGLASIGQWWLNPIAFEKAILWNMMFEGLGLGCG
                     FGPLTGRYFPPIGGVLYFVRPGTTKLAVFPRLPVLGGTRRTWLDVGLYVALLALVLRA
                     LVAPAPGASELVPIAVLVPVLGLADRTIFLALRAEHYWTTIVCLAAGGNWIAGAKAVQ
                     LALWFWAGFSKLNHHFPTVVCVMVSNSPVLRLPWLRRRMYRRFPDDLRPSWLAVAMSH
                     AGTALELAVPLILLVSPGGASLLVGFALMLVLHTFITSNVPLGVPIEWNVMVVYGAFA
                     LFWAHPEITVQGVSSPALAVFLVVMLIGAPLVGNLAPGALSFLLAMRFYAGNWPYGVW
                     LFRGDSYRKLDRLTKTAPWVHDQLGRFYDRSTAVGLLGKVMGFRLMHLQGRALPVLIP
                     RAVDRLEDYEYLDGEIVAGLVLGWNFGDGHLHRESLLAAVQAQCGFAPGELRCIFVES
                     QPLGRATMAYRIHDAATGQLDAGVLDVRAMRRRQPWAAA"
BASE COUNT          791 a         1998 c         1921 g          845 t
ORIGIN      
        1 gggccgtgga cctgctggtg gacgacctgc agctcggcct ggatcagggt gtcggccgac
       61 gggatgtaca gcttgccggt cgcgcctccg cggacccgcg cgaggccgtc gctcgacgtc
      121 accatcagct cgccgccgag ctgcagccgc gggttgagcc ggacctcggc gtagcccgcc
      181 ccgccgctgt cgtgcacgac ggtgtcgatc agcgggtcct cgaggaagcc ggtcacgtgc
      241 gcttcccagg tgtccttcac gtactcgacc gcggcgccgt aggtctcgcc gtacagcggc
      301 gtgccgccga accgccgggt gtagatcggg tgctcggcga accgcagccc gaacaccggc
      361 atgaaccggc cgacccggac gaacaggcca tcgtggccgt tctcgtcctg ctgccacatc
      421 acgtagtgct cgcgcgacca gaaccggtcg acgatcgccg gcgtgttgtt gacgcccacc
      481 cattgcgccg ggcgcccccc gacggtgacg tgcagcgtga agcccttggc cgtcgcgctg
      541 gcgtagacgt cggcctgcat cgggatgagc gcgaagctgc cgtccggcgc cttgaagtag
      601 ccggtgctgc cgcgcaggtc gccgccgagc gcgagccagc cgggcagctt gaacagcccg
      661 tacatgaacg ccgacgaatc gccgagcagc gcctgggtct cggcgacgct gtagccgttc
      721 tcggtgagca gcccgccgcc cgcgggcgac aggtgacagc cagtgcaggt ctgatcgcgg
      781 ctgagctggt actggggata ggccgcggcg cgccggctcg cggccccgac cagcgccagg
      841 gcgatgacga cgactactgc tttgtgcatt gtccattccc ggtggtggtc gagcagaccg
      901 actgctgctt gttgctggcc gggtccgcga gcggacagtc catgacgacg gcgccgatgt
      961 tgccatcgac gcactggacg acgtggtaga cgcggccggc gcccgtcccg gtgaccgagc
     1021 agccgttgcc ctggtcgttg gccaggcact gcgcaccggg agccccgtcc tggatccacg
     1081 ccgcgatcag ggcgacgtcg gcgtccggca tcggtgcgtc gaacggcatc cgcacggtgg
     1141 agcccgggct caggatgctc ggcgcgccga tcgtgatcga cgtgtagaga acggacgagc
     1201 ccggattccc cgggttcacc aggccgttgt tgacgatcgt ccggcgggtc gcgtccaccg
     1261 tgtcgaactc gtcgccgacc tccctcttga aggccgagtg gcactcggcg gccgcgcagg
     1321 tcggcgcgag gatggtctgg gtgatgtact ccaggttccg gggccggtcg tcggtcgcgc
     1381 cgcatgcccc agccgcgctg ccgagcagcg cgccgatcag cacgagctgt ttcatggccc
     1441 cgaccctact ccagcggcgc ggcgcgcacc acttcgtggc ggggggctct gcgttacgga
     1501 tcgttagcgg gcttgcgttc cgcgtgcggc acgggtccgg cgccgtccgc cccgtccgcc
     1561 ccgtccgcga tctggatcac cgccgcggcg ccccgcggcc gacgaaccag gtacagcgcc
     1621 gccccgatca ggttcaccgc cacgacggtg ccgccgatct tggcgacgaa cgccagccgg
     1681 ctctcgacct ccacgatcgg cagcaccgac agcacgacga acagcgacgt ggtcgcgaac
     1741 ccgaccgccg cggcgatccg gatccacggc ggcgcgcgcc ggccggtccg cgccaggccg
     1801 accagcggca ccgcgaacag caccaggtag gtcagcccgt acaggatccc cgcggcgttg
     1861 tcgagcagct ggaacgcctc ctgctcgccg accccgagca tcccgaggat cgcgaacacc
     1921 agcccggcgc cgccgatgaa cagcaccgag ttcaccggcg tgcggaaccg cgcatgcagc
     1981 cgcgagaacc aggccgggat cagccggtcc cagcccgcga ccatcggcat ccgcgaggtc
     2041 gcggtgaaca gcaggctcga gttgccgatc gaccgcgcgc tcaccgcgaa caccgccagc
     2101 gggatcagga tcgcgaccgc gcccgagccg gggagcccgt tgcggaacgc ctgggggatc
     2161 gggccgacca gatcgacctg gtccggtggg gtcagcgcct ggatcgccga ggtccccagg
     2221 atgaacatca gcgcgatgac cggcgccgcg atcatcacgc tgcgtccgat gttgcggccc
     2281 gcgttgcggg tctcaccggc gagcacggcg acgtactcga agccggacag cgcgccgacc
     2341 gcgagcttgc cgaagatgtt caggttgaac acggtcagcg ccggcgccgt gaccgccagc
     2401 gggtgatact cgcgcagcgt gccgcgcgcg acgttgatcg cgggcagcgc gagcaacagg
     2461 ccgaacgcga gcagcagcag caccgcgccg gcgttgtgca cccacttgct ggtccgcagg
     2521 ccgaggatcg acagcacggt cagcgcgccg atcagaccgc cgctcacggc acccatgaac
     2581 cagtgattgc ccgacagcga ctgcgccgcc gggccgatcg cgtagatgat cgagttggcg
     2641 aactgcagcc cgaacgcgcc gagcagcacg atcgcgtaga tccacaggtt ccagccgacg
     2701 aggaacgcca tgccctcgga gaggccgagc ttggaccact ggtacagccc gccctcgagc
     2761 gggaaccgcc gcgacaggtg gaccacgacg atcgcctgcg gcacgtagaa catcgccatc
     2821 gcaccgagcc agaacgggat ctgcgacgtg ccgagcttgg cggcggtgcc gacccagctc
     2881 aggccgacga cgtagagaat ctgggtcagc accagatcgg tgaggcccag ctcctgccgc
     2941 agctggctgc tctgctgttc gacggtcgac atggtcccct cttttgcata gacccggatc
     3001 gccccagccc cgtgcggcgg cggttgtagc atggcggctc ccggcttcgt cccgctggct
     3061 acctgctcgg acccacagcc gcttgcagct cggcggcaaa ttaccgtcag ccacgacacc
     3121 gatctatcac cgtattccgc actgccgcga caccgtgcga cggtgctaca gcccctggtc
     3181 tcgggcagtc cactccccct caggattccg agtgatcacc gccggcattc atcgagtctt
     3241 tccttggccc gtgtcggggc cgctggcatc atcttcgcca gtgcgctgcc ttgcacgatc
     3301 gtgaagggga tcaggacatg ggatcgacca agcacagctt cgtccactcc atccaaccgt
     3361 cggcgctgcc tccgccgtac cagctggcga tcgaggacgc cgagttccag ttgaccttgc
     3421 tggaggacgg cgcgccgcgc agccacgatc ccggctgcgc ggtcgtgccg ccgacctggt
     3481 tctcggtcgc gctgaccgcg ccgacccgca agcgctacct gtacctggtc cagaaggaga
     3541 ccgacaccgg cctgatcgtg gtgatcatgc tgttcgaccg cagcgcgacc ggcagcgacg
     3601 ccgcggggct gcggttgcca tcgagcggcg cctggctgcg cgcggtggtc gacggcacgg
     3661 tctacgtcct cgcctccgac ctcgtgctga ctcgcaaggc gatcaccgcc tggatcggcg
     3721 ggcgcgagcc gccgaccacg cctccgcagc cgccgtacac ttgatccggg cctgatccga
     3781 aatctgatcg cggccaggcg ggcgcgccga accggtataa cgtagcggcg tgagcctgct
     3841 agcaccgaca cctgcgccct acgatccgct ggactgggtg cagcggccgt tcgccgagcg
     3901 cgcccggatg gtgtgcgagg cctgggcgat ccagggctac ggcaccccgc cgctgatcta
     3961 cgcggtccac gtggtgaaga tcgcgctgta cgtcggcgcc tggctgatgt tctgcagcac
     4021 ctcgccgggg ctcggcggcc tggcctcgat cggccagtgg tggctgaatc cgatcgcgtt
     4081 cgagaaggcg atcctgtgga acatgatgtt cgaggggctc ggcctgggct gcgggttcgg
     4141 gccgctgacc ggccgctact tcccgccgat cggcggcgtc ctgtacttcg tgcgccccgg
     4201 caccaccaag ctcgcggtgt tcccccgcct gcccgtgctc ggcggcaccc ggcggacctg
     4261 gctcgacgtc gggctctacg tcgcgctgct cgcgctggtg ctccgcgcgc tggtcgcgcc
     4321 ggcgccgggc gcctcggagc tcgtgccgat cgcggtgctc gtcccggtgc tcggcctggc
     4381 cgatcgcacc atcttcctgg cgctgcgcgc cgagcactac tggacgacga tcgtgtgcct
     4441 ggcggcgggc ggcaactgga tcgccggcgc caaggcggtc cagctcgcgc tgtggttctg
     4501 ggccgggttc tccaagctca accaccactt cccgaccgtg gtgtgcgtga tggtcagcaa
     4561 cagcccggtg ctgcggctgc cctggctgcg gcggcggatg taccgccggt tccccgacga
     4621 cctgcggccg tcgtggctcg cggtcgcgat gagccacgcc ggcaccgcgc tcgagctcgc
     4681 ggtgccgctc atcctgctgg tgtcgccggg cggcgcgtcg ctgctggtcg gcttcgcgct
     4741 catgctcgtc ctccacacgt tcatcaccag caacgtgccg ctcggcgtgc cgatcgagtg
     4801 gaacgtcatg gtggtgtatg gcgcgttcgc gctgttctgg gcccaccccg agatcaccgt
     4861 gcagggcgtc agctcgccgg cgctcgcggt gttcctcgtg gtgatgctga tcggcgcgcc
     4921 gctggtcggc aacctcgcgc ccggcgcgct gtcattcctg ctcgcgatgc ggttctacgc
     4981 cggcaactgg ccgtacggcg tgtggctgtt ccgcggcgac agctaccgca agctcgaccg
     5041 gctgaccaag accgcgccgt gggtccacga ccagctgggg cggttctacg accgctcgac
     5101 cgcggtcggc ctgctcggca aggtcatggg gttccggctc atgcacctgc agggccgcgc
     5161 gctgccggtg ctgatcccgc gggcggtcga ccggctggag gactacgagt acctcgacgg
     5221 cgagatcgtc gcgggcctgg tgctgggctg gaacttcggc gacggccacc tccaccgcga
     5281 gtcgctgctc gccgcggtgc aggcacagtg cgggttcgcc cccggcgagc tgcgctgcat
     5341 cttcgtcgag agtcaaccgc tcggccgcgc gaccatggcg taccggatcc acgacgcggc
     5401 gaccggccag ctcgacgccg gggtgctcga cgtgcgcgcg atgcggcggc gccagccgtg
     5461 ggcggccgca tgaccggccg accggacagc gtcgtgctga tcgatcgccg ggacgactca
     5521 gccgcgcgac gcgacgcgcg gaggggtgaa tttcg
//
LOCUS       DASVOR010000514         6000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238345,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000514 DASVOR010000000
VERSION     DASVOR010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6000
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238345"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(293..475)
                     /locus_tag="VF516_10270"
     CDS             complement(293..475)
                     /locus_tag="VF516_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108102.1"
                     /translation="MLTFGDRLEVLQLGIPPEEVVIKDRLPDDILDQHDRIVVQRFCC
                     TARLLYNKIVGLNPRI"
     gene            711..1553
                     /locus_tag="VF516_10275"
     CDS             711..1553
                     /locus_tag="VF516_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108103.1"
                     /translation="MKVAGSGVDLETRAPSWQEDADRAFVAIVKSETPLLILIDELPV
                     LVLALAKADTSGARVRAFLQWFRNLRQQIAGASKLRFVIAGSIGLDNVTRRHQLTETI
                     NDLRLWPLGPFQPAAAHEFLGQLAASYEMDLGPELRTAVCGVAEWLIPYHLQVIFSEL
                     REHSKATQPTMATLNATIETLLDRRTYFSYWDERLHDALGEPSASIARAMLGICAQDP
                     AGTTPEAMNQSIAAIVPDPVERAKAFKWTVDVLSNDGYLVLQDGRWRFRSGLLRRYWV
                     RHVV"
     gene            1550..3847
                     /locus_tag="VF516_10280"
     CDS             1550..3847
                     /locus_tag="VF516_10280"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108104.1"
                     /translation="MTIYNPHLLARQELIETFVARGPLLDELIDDLRRGGRQHHLLVG
                     GRGSGKTTLLLRLAAAIEDNQQLTRKCIPLRFPEEQYNVSRPSDFWMNAIDALADALE
                     RQGDSIAAKRLESSLVELEPLDEAERSRRSLALLESWAKQTKRLVVLLIDNFDLVLDR
                     LAESLWALREALSLDNRLVVIGASSRFVEEAINYQSPFYDFFRVHELGPLSEEEARAV
                     VLSLARRANTPKVAEVVERDPGRFMALFVLTGGTPRTLALLHTVLALEQRESVERDLD
                     GLLDQLTPYYKARFDDLPPQSQVIVDKVALHWHPITAAECQAATHLHVNVVSAQLNRL
                     VKGGILTRVSLPGPGKLGFQLSERFFNIWYLMRASRRLRRRLSWFVEFLRIFYGDEDL
                     RQRAEELIRAAPAESLTNPAKFLAFATAIPDEALRRRLEFRAISLLADKGAIALQELM
                     DLEGEDVHLVPVIDRVRALRDIRARIAAATVPWPSGLTSSDIGEAIARAATLPMILKR
                     EAARAVEEASADMVDNMCRLVKSLQENPNNIPERLLAAVSTGEVPAYGDVRDLDEANQ
                     IIELMGSRPQAIVSIVLCTEIAQNPLPSHIIEQLVGSDPRDSYAVILTAKALVGLNDW
                     SSLRRLILLAVRHNTSEIQLGLFVSFLRRCVSTGLAQEALDLVIEAGLTERWAPLYEA
                     LRAVVEDDASRLDSLAPEMRTIARSLYDQLRTPDLPKLGNAQMVAPGSAPTKQKPIRR
                     RAGRKAPRPEGGKRTSRRPARAKPP"
     gene            complement(4010..4558)
                     /locus_tag="VF516_10285"
     CDS             complement(4010..4558)
                     /locus_tag="VF516_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108105.1"
                     /translation="MQTLPTISVFSLGVVGLMACATTPSGTPSSGPPDPPTGEADLEI
                     RHSGTAVVGSSFQCTGNWPSALTPCGYAWTSQPQTVATGDGAGKVRLVLRRSPMPVDG
                     GASTVYIDLSFDASGKPTATAQESTTRPPLAREVETSKPISGWIDPVAMSAASDARQA
                     GTFSLTFEWGTISGTYDTAPAQ"
     gene            4844..5833
                     /locus_tag="VF516_10290"
     CDS             4844..5833
                     /locus_tag="VF516_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108106.1"
                     /translation="MTKEAKTEAKAKAKPDAKTEADTEAKADAKTEAKTESKTEAKTE
                     AETDAAPAPPAAGIPRKTWIIIGVIVVAVWAFAINTHNTIVLIVAGVLTALLAGVLLW
                     ALRTIRKHRSTVSLLQGAVASPEARREALAKLSEGKDAKSPTNVFARAQLLAADDDPQ
                     GALKLLGTIELRSYPPAMQDDVSLLQTQLYLRLGRTLDARKSADVMNLDNPQRKEIRP
                     LAASIVAEAWARTGKPKEALVLLDTIELPRKDAEQIALQIRVARVFAKFAANQRGPAR
                     AELVALADDDFNQLGRFIQPQFRVHPELQKLARSVIEQHPAARRHITAQTKRR"
BASE COUNT         1081 a         1991 c         1929 g          999 t
ORIGIN      
        1 ctctgccgcc gtgtacttcc gccgcttccg cggctcatct gcctccgctg tcatctgcgt
       61 cctcgttggg acgagtaccc accctcaggt cgtccggggt ttcaggtctc gatcgccgtg
      121 gacgatgttc ggtaacgaca cctaggagcg acctgcctgt cttcggggtg aagccaacga
      181 cccaagggag tctgccgacc ccgcggggga gctccgatca cgcagcagtg aagcggcctc
      241 cccatgagcc ccagggccag gctaagatct ccatcataag atactgtttt tactatattc
      301 ttggattcaa gccaacaatt ttgttgtaca acaatcttgc cgtacaacaa aaacgttgta
      361 caacaatcct gtcgtgctgg tcgagaatgt cgtcgggaag ccggtccttg atcacaactt
      421 cttcgggcgg gatgccgagt tggaggacct ccaggcgatc accgaacgtg agcacgtgct
      481 gctcctggca cccaggcggg tcggcaagac ctccctgcta cacgcgctgg ccaaggcgcg
      541 tcgatcgcga cgggtccgcg atcggcgtct atgcgagcgt ggcggcagcg acgaacgaga
      601 gccagttcgt gcaagccatc ctgagcgcga tctacgcgac caagaagggt cgaaagctca
      661 agcgcgggtt cgtcgcgcgc acccttggcc tcggtcgggg cgtcaagagc gtgaaggtcg
      721 ccggcagcgg cgtcgatctg gagacacgcg caccatcctg gcaagaagac gccgaccgcg
      781 cattcgtcgc gatcgtgaag agcgagaccc cgctcctgat cctgatcgac gagctacccg
      841 tgctcgtcct tgcgctcgcc aaggccgata cctcaggtgc acgcgtacgt gcgttccttc
      901 aatggttccg caacctccgg caacagatcg ccggggcttc caagctgcgc ttcgtgattg
      961 ccggatcgat cgggctcgac aacgtcacgc ggcgtcacca gctcaccgag acgatcaacg
     1021 atctccggct gtggccactc gggccgttcc agccggcagc ggcccacgag ttcctcggcc
     1081 agctcgcagc cagctacgag atggacctgg gtccagagct ccgcaccgcc gtctgcgggg
     1141 tcgccgaatg gctgatcccg taccacctcc aggtcatctt cagcgagctg cgcgagcaca
     1201 gcaaggcgac gcagcccacg atggcgacct tgaacgccac gatcgagacc ttgctcgacc
     1261 gacggaccta cttctcttat tgggacgagc gtctccacga tgcgcttggg gagccgagcg
     1321 catcgatcgc gcgggccatg cttggcattt gcgcccagga tccggcaggc acaacgcccg
     1381 aagcgatgaa ccagagcatc gccgccatcg tcccggatcc ggtcgagcga gccaaggcat
     1441 tcaagtggac cgtcgatgtg ctctcgaatg acggctacct ggtcttgcag gacggtcgct
     1501 ggcgctttcg ctccggcctg ctgcggcgct actgggtgag gcacgtggtg tgacgatcta
     1561 caacccacat ctcctcgccc gacaggaact gatcgagact ttcgtcgcca gagggcccct
     1621 gctcgacgag ctcatcgatg atctccggcg aggaggtcga cagcatcacc tcctcgtcgg
     1681 gggccgtggt tccggcaaga ctaccctcct actccgtctc gcagcagcca tcgaggacaa
     1741 ccagcagctc acaaggaagt gcatcccact tcgcttcccc gaggagcagt acaacgtctc
     1801 tcgcccctcc gacttctgga tgaacgcgat cgacgcactc gccgatgccc tcgagcgcca
     1861 gggcgacagc atcgcggcga agcgtctcga gtcgtcgctc gtcgagctgg agcctctcga
     1921 tgaggcggag cggtcgaggc ggtccctcgc actgctcgaa agctgggcca agcagacgaa
     1981 acggttggtc gtgttgctca tcgacaactt cgatctcgtc ctcgaccggc tggcagagtc
     2041 gctgtgggcg ctacgcgagg cgctgtccct cgacaaccgc ctggtcgtga tcggcgcctc
     2101 gtcgaggttc gtcgaggagg cgatcaacta ccagtcaccg ttctatgact tcttccgggt
     2161 tcacgagctc ggaccactgt ccgaggagga ggcgcgcgcc gtcgtgctgt cgctggctcg
     2221 acgtgccaac acgccgaagg tggccgaggt cgtcgagcgc gaccctggcc gcttcatggc
     2281 gctctttgtc ctgacgggtg gcacgccgcg aacgctcgcc ttgttgcaca cggtgctggc
     2341 gctggagcag cgcgagagcg tcgaacgcga cctcgacggc ctcctcgacc agctcacacc
     2401 gtactacaag gcgcgcttcg acgaccttcc accccagagc caggtaatcg tcgacaaagt
     2461 ggcgctgcat tggcatccca tcacggcggc agaatgccaa gcagcgacgc atctgcacgt
     2521 caacgtcgtc tccgcgcagc tcaaccggct cgtgaaaggt gggatactca ccagggtttc
     2581 attgccggga ccgggcaagc tcggcttcca gctcagcgag cgattcttca atatctggta
     2641 tctcatgcgc gcgagccgcc gcctgcgccg ccggctgtct tggttcgtgg agttcctgcg
     2701 gatcttttat ggcgatgagg atctgcggca gcgcgccgag gagctcatcc gggcggcgcc
     2761 agctgagagc ttgacgaatc cagcgaagtt cctcgcgttt gccactgcca tccccgacga
     2821 ggcgctccgc cgccggctgg agttccgggc gatctctctt ctggcggaca agggcgcgat
     2881 tgcgctccaa gagctgatgg acctggaagg cgaagacgtc cacctggtgc cggtcatcga
     2941 tcgcgtgcgc gccctgagag acatccgcgc gcggatcgcc gcggccactg ttccgtggcc
     3001 gtcaggcctg acctcgagcg atataggaga ggcgattgca cgagctgcga cattgccaat
     3061 gatattgaag cgagaagcag ctcgcgccgt agaagaagcc tcggccgaca tggtcgacaa
     3121 catgtgccgt cttgtcaaga gtttacaaga aaatccgaac aatataccag agcgactcct
     3181 cgctgctgtt tcgactggtg aggtaccagc ctatggtgac gtccgtgatc tcgatgaggc
     3241 caatcagatc atcgagctca tgggatctcg tccccaggcg atcgtatcta tagtgctttg
     3301 caccgaaatt gcgcagaatc cccttccgag tcatattatc gagcaactag ttggttcgga
     3361 tccgcgtgat agttatgcgg tcatcctgac cgccaaggct ctcgttggcc taaatgactg
     3421 gagcagcctg cgacgcctca tcctgctggc tgttcgacat aatacgtcgg aaatacaact
     3481 tgggctattt gtctcttttc tcagaagatg tgtctctact ggactggcac aagaagccct
     3541 cgacctggtg atcgaggccg gtctgacgga acgctgggct ccgctctatg aggcacttcg
     3601 cgcggtggta gaagacgatg cttcgcggct agacagcctt gccccagaaa tgcgaacgat
     3661 agcccgttcg ttgtatgatc agcttcgaac gccggatctc cccaagctgg ggaatgcgca
     3721 gatggtcgct cctggcagcg caccaacgaa gcaaaaaccg ataagaagac gggcaggtcg
     3781 caaagctccg cgacctgagg gtggcaagcg aacgagccga cggccggcga gagcgaagcc
     3841 gccctgatca gcccagcacc aggtcgggca caagctcaag cgggtcccgc caggaacgat
     3901 ctccatctgc ggcggccccg tcaacgcgcg cgctggacca tcaccggcga ttcctggtga
     3961 gcacgctggt cacggatcgg ccccgccctg ccgtgtcgtg cggggcagtt tactgcgccg
     4021 gtgcggtgtc gtacgtgccg ctgatcgtgc cccactcgaa ggtgagcgag aacgtcccgg
     4081 cctggcgcgc gtcgctggcg gcgctcatcg cgaccggatc gatccagccc gagatcggct
     4141 tgctggtctc gacctccctg gcgagcggcg ggcgggtcgt cgactcctgc gcggtggcgg
     4201 tgggcttgcc gctggcgtcg aagctcaggt cgatgtagac ggtcgaggcg ccgccgtcga
     4261 ccggcatcgg ggagcggcgc agcaccaggc ggaccttgcc ggcgccatcg ccggtcgcga
     4321 cggtctgcgg ctgcgaggtc cacgcgtagc cacacggcgt cagcgcgctg ggccagttgc
     4381 cggtgcactg gaacgagctg ccgaccaccg cggtgccgga gtgccggatc tcgaggtccg
     4441 cctccccggt gggaggatcc ggcggaccgc tggaaggcgt gccggacggc gtggtcgcac
     4501 acgccatcag cccgaccacg ccgagcgaga agacggagat cgtgggaagc gtctgcatgc
     4561 tgcctcctgg gtgaattctg cgctgccgcg gacgagccgg cagccgtgtc tcgattcact
     4621 gcgagaaagt cgtcgaagaa cgcagagccc tgacagcggc tgcccgattg tggcgccacc
     4681 gcggggcgag gactgccgca ccgtgcggca aagatatggc aagccgccgc gcgcagcgcc
     4741 agcctcgcgc gggtctggac aagccatcta gatcatttgg gaacttggga tgccatgggg
     4801 tgtgctttgg atcgcggcgg cgaccgtgta gacaaggacc accgtgacga aggaagccaa
     4861 gaccgaggcc aaagcaaagg ccaagccgga cgccaagacc gaggccgaca ccgaggccaa
     4921 ggccgacgcc aagaccgagg ccaagaccga gtccaagacc gaggccaaga ccgaggccga
     4981 gaccgacgcc gcgccggcgc cgcccgccgc cggcatcccg cggaagacgt ggatcatcat
     5041 cggcgtgatc gtggtcgcgg tgtgggcgtt cgcgatcaac acgcacaaca cgatcgtgct
     5101 gatcgtcgcg ggcgtgctga ccgcgctgct cgccggcgtc ctgctgtggg cgctgcgcac
     5161 gatccgcaag caccggagca cggtgtcgct gctgcagggc gcggtcgcct cgcccgaggc
     5221 gcgccgcgag gcgctcgcca agctgtcgga gggcaaggac gccaagtcgc cgaccaacgt
     5281 gttcgcccgc gcgcagctgc tcgcggccga cgacgatccg cagggcgcgc tcaagctgct
     5341 cggcacgatc gagctgcgca gctacccgcc ggcgatgcag gacgacgtgt cgctgctgca
     5401 gacccagctg tacctgcggc tcggccgcac gctcgacgcc cgcaagtcgg ccgacgtgat
     5461 gaacctcgac aacccgcagc gcaaggagat ccggccgctc gccgcgagca tcgtcgccga
     5521 ggcctgggcg cgcaccggca agcccaagga ggcgctcgtg ctgctcgaca cgatcgagct
     5581 gcccaggaag gacgccgagc agatcgcgct gcagatccgg gtcgcgcggg tgttcgcgaa
     5641 gttcgcggcc aaccagcgcg gcccggcgcg cgccgagctg gtcgcgctcg ccgacgacga
     5701 cttcaaccag ctcggccggt tcatccagcc gcagttccgc gtccaccccg agctccagaa
     5761 gctcgcgcgc agcgtgatcg agcagcatcc ggcggcgcgc cgccacatca cggcgcagac
     5821 caagcggcgg tgagcgaggg acccgtgccc gcgctcagga cggaggcggc gagaccgcgc
     5881 cctggatcgt ggcaggctgc gtgctgaaga tgcggatcac gccggtgccg ccgccgcccc
     5941 cgccgcccgc gcactcctgc gccgtgttgt tgccaccgcc cttggtgccg gccgcgcccg
//
LOCUS       DASVOR010000515         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_384575,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000515 DASVOR010000000
VERSION     DASVOR010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384575"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1545)
                     /locus_tag="VF516_10295"
     CDS             complement(34..1545)
                     /locus_tag="VF516_10295"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX8108107.1"
                     /translation="MRHPAITIGAAALAVCATASAAHAFCGFYVSGSGEKMFNDATNV
                     VLMRDGTRTVLSMQNDYKGPLEDFAMVVPVPVVLHEPDVKTLAKTVFDHTDALGSPRL
                     VEYWEQDPCPMPVERAYDMAPRAAAAPTGSAAGDFAMAKKESVRIEAKFDVGEYKILI
                     LSATDSAGLETWLHQNHYQIPPGAEPLLRPYVEGGSKFFVAKVDPKKVVMQDGHAALS
                     PLRFHYDSEEFALPIRLGMANSSGKQDLIVNIISPDQRYEVANYRNVFIPTNFDVKNE
                     VKTRFAEFYAALFDKTIEANPGAVVTEYAWTAVPNYHCDPCTDADVSNPDLMTLGGDV
                     VGGRIARGNFVLTRLHARYGKADMKDDLRFRVARPVTGGREQWSKQGIEYGATPSPQN
                     YFQARYAIRHAWTGPIRCEHPVRNVWGGPPDGNYQPTIAASKLAFAPRGKLEVASVVK
                     RDLWEIGLKRGSAPGGGKFATPPNPRLQGVGLIALLGLLGASAVFAVRRRSRS"
     gene            complement(1597..>2356)
                     /locus_tag="VF516_10300"
     CDS             complement(1597..>2356)
                     /locus_tag="VF516_10300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108108.1"
                     /translation="LLGAPSTAPAAADPYILAGEASALLHRDGALRVIPPDAYTRAYA
                     ANPAPWRPELPEVLAGLVARGLRVGFISNSDRLAIEMRLTDVLHADRALRRQIVVHGH
                     AAKFTLGELPIGATGHGAAHRARFEALDGSVRAAGTVRPIYLRRGAYFDALCGLWRDL
                     GATDYAIAETLVCGDIWELDLAMPRALGAHVHLIRRAPPFDTYPYELAQLPPADISGD
                     LRGLLDHVDRLRTGPDRGGDPAGFARGEGQSPLE"
BASE COUNT          322 a          825 c          852 g          357 t
ORIGIN      
        1 gaccgcgaga acgacgagcg cgccccgcat cgcctagctc cggctccggc ggcgcaccgc
       61 gaacaccgcg gacgcgccga gcaggccgag cagcgcgatc agcccgacgc cctgcagccg
      121 cgggttcggc ggcgtggcga acttgccgcc gcccggtgcg ctgccccgct tgagcccgat
      181 ctcccacagg tcgcgcttga cgacgctggc gacctcgagc ttgccgcgcg gcgcgaacgc
      241 gagcttgctc gcggcgatcg tcggctggta gttgccgtcg ggcgggccgc cccacacgtt
      301 gcgcaccggg tgctcgcacc ggatcgggcc ggtccacgcg tggcggatcg cgtagcgcgc
      361 ctggaagtag ttctgcggcg acggcgtcgc accgtactcg atgccctgct tcgaccactg
      421 ctcgcggccg ccggtgaccg gcctggccac gcggaaccgc aggtcgtcct tcatgtcggc
      481 cttgccgtag cgcgcgtgca gccgggtcag cacgaagttg ccccgcgcga tccggccgcc
      541 gaccacgtcg ccgccgagcg tcatcagatc ggggttcgag acgtcggcgt cggtgcacgg
      601 atcgcagtga tagttgggga ccgcagtcca cgcgtactcg gtgaccaccg cgccggggtt
      661 ggcctcgatc gtcttgtcga acagcgcggc gtagaactcg gcgaaccggg tcttgacctc
      721 gttcttgacg tcgaagttgg tcgggatgaa cacgttccgg tagttcgcga cctcgtagcg
      781 ctggtcgggc gagatgatgt tgacgatcag gtcctgcttg cccgacgagt tggccatccc
      841 gagccggatc ggcagcgcga actcctcgct gtcgtagtgg aaccgcagcg gcgacagcgc
      901 ggcgtggccg tcctgcatca cgaccttctt cggatcgacc ttggcgacga agaacttcga
      961 gccgccctcg acgtagggcc gcagcagcgg ctcggcgccc ggcgggatct ggtagtggtt
     1021 ctggtggagc caggtctcca ggcccgccga gtcggtcgcc gacaggatca ggatcttgta
     1081 ctcgccgacg tcgaacttgg cctcgatccg caccgactcc ttcttcgcca tcgcgaagtc
     1141 gccggccgcg ctgcccgtcg gcgccgccgc cgcgcgcggc gccatgtcat aggcccgctc
     1201 gaccggcatc gggcacgggt cctgctccca gtactcgacc agccgcggcg agccgagcgc
     1261 gtcggtgtgg tcgaacacgg tcttggccag ggtcttgacg tcgggctcgt ggagcacgac
     1321 cgggaccggg acgaccatcg cgaagtcctc gagcgggccc ttgtagtcgt tctgcatcga
     1381 cagcacggtc cgcgtgccgt cgcgcatcag cacgacgttg gtcgcgtcgt tgaacatctt
     1441 ctcgcccgag ccgctgacgt agaagccgca gaatgcgtgg gccgcgctcg cggtggcgca
     1501 caccgccagc gcggcggcgc cgatcgtgat tgctgggtgt ctcatgagtc ctctggtttc
     1561 ctgtcgggct tccaacgctt caccggtcgc cccggtctat tccaaggggc tctgcccctc
     1621 cccacgggca aagcccgcgg ggtcccctcc ccgatctggt cccgtgcgca gccggtcgac
     1681 gtggtcgagc aggccgcgca ggtcgccgct gatgtcggcg ggcggcaact gggcgagctc
     1741 gtaggggtag gtatcgaacg gcggcgcgcg gcggatcagg tggacgtggg cgccgagcgc
     1801 gcgcggcatc gcgagatcga gctcccagat gtcgccgcag accagggtct cggcgatcgc
     1861 gtagtcggtc gcgccgagat cgcgccacag cccgcacagc gcatcgaagt aagcgccgcg
     1921 ccgcaggtag atcggccgga cggtgccggc ggcgcggacc gagccgtcga gcgcctcgaa
     1981 ccgcgcgcgg tgggccgcgc cgtggccggt cgcgccgatc ggcagctcgc ccagcgtgaa
     2041 cttggcggcg tggccgtgca cgacgatctg tcggcgcagc gcgcgatcgg cgtgcagcac
     2101 atcggtcagc cgcatctcga tcgccaggcg atcggagttc gagatgaagc cgacccggag
     2161 gccgcgtgcg accaggccgg ccagcacctc ggggagctcg ggccgccacg gcgccgggtt
     2221 cgcggcgtag gcccgggtgt aggcatccgg tgggatcacc cggagcgcac cgtcgcggtg
     2281 gagcagcgcg ctggcctcgc cggcgaggat gtacggatcg gcggccgccg gcgcggtcga
     2341 cggcgcgccg agcagc
//
LOCUS       DASVOR010000516         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_4863,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000516 DASVOR010000000
VERSION     DASVOR010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4863"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF516_10305"
     CDS             <1..216
                     /locus_tag="VF516_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108109.1"
                     /translation="AISIAAVAAAPFTGGASLAILLPVGVIGAVPSAYRLATRAENGT
                     LRFPRHRRRRASARFCAATAAPRQRQR"
     gene            213..839
                     /locus_tag="VF516_10310"
     CDS             213..839
                     /locus_tag="VF516_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108110.1"
                     /translation="MIDAVFRYLFDSSGIAFTYENYAAWERLAGGRGTLDDVRFLVHE
                     MAEVGILQHEGVDFMGDRYTVDSPAHEDWFKRTFEPAYARAHAQALQVEFQFISEQLA
                     RVTEGRLQLSPAEIAAVDTVNAQAREFLLVDGMPVDRHPRYREWFGRATAPVELSPAM
                     RQRIGLPEGRDITIEQLVRAIKQAQVTGGEFSRSSSEPLYLGPSLQPP"
     gene            1062..1292
                     /locus_tag="VF516_10315"
     CDS             1062..1292
                     /locus_tag="VF516_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108111.1"
                     /translation="MAALRAALGKLEPVELRDVPVSLSANELSQLALQHGVTSNQWGK
                     EAMLDYFAQLRERNLLPRGDVLEDPAFYRDHP"
     gene            1342..1767
                     /locus_tag="VF516_10320"
     CDS             1342..1767
                     /locus_tag="VF516_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108112.1"
                     /translation="MKFFLTWLSSADVQERATALRSQANRPLADPRLLARAEELLNDT
                     TLCRLEIPLLYGEIRWRAAQAVAALRAALGKVEPVELRDVLVPLRANKACQLAEQHGA
                     TSARAGNDGVLECLAQLRERGLLPRRDIVEDPAFYRAPP"
     gene            2200..2661
                     /locus_tag="VF516_10325"
     CDS             2200..2661
                     /locus_tag="VF516_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108113.1"
                     /translation="MLSGGDGDPDVPCIIDTGPGCAPGTSIGSPAGTCTTTEIANARL
                     NGVNFWTCQNGDRYICDDHSHKIRETCINGCVSHPAGADDECAFGSAVQCTSAEFSGQ
                     NLLVNGFNASFWTCQGAARYVCDGASAHYKVKESCPNGCQGAGVGRDDQCL"
     gene            complement(2672..2965)
                     /locus_tag="VF516_10330"
     CDS             complement(2672..2965)
                     /locus_tag="VF516_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108114.1"
                     /translation="MRFRAVLASLALSAGCGGALPVVTAGDAARSGVALAELQQGRQL
                     VAAKCSSCHRTPQPAEHRSDEWPHMIDEMSRRAHVDLSERQAIQDYLVTMAAR"
     gene            complement(3043..>3591)
                     /locus_tag="VF516_10335"
     CDS             complement(3043..>3591)
                     /locus_tag="VF516_10335"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8108115.1"
                     /translation="HFFLRLEEAMADDAVLVVDGGDFVATASYIVRPRAPLSWLDPGA
                     FGTLGVGGGFALGAAMVRPGKEVWLVWGDGSSAYTLAEFDSYVRHGVAPIAVIGNDAS
                     WMQIAREQVEVLGTTLGCDLRRTDYHKVAEGYGGVGLALTDGARVDGVLAEAKAIART
                     GRPVCINVHLRKTDFRKGSISM"
BASE COUNT          553 a         1274 c         1224 g          540 t
ORIGIN      
        1 gcgatctcga tcgccgccgt cgccgccgcg ccgttcaccg gcggcgcctc tctggcgatc
       61 ctgctgccgg tcggcgtcat cggcgcggtc ccgtcggcct accgcctggc gacccgcgcc
      121 gagaacggca cgctgcgctt cccgcgccac cgacgtcgcc gcgcgtcggc gaggttttgc
      181 gcggctacgg ccgcgccgag gcaacgccag cgatgatcga cgcggtgttc cgctacctgt
      241 tcgacagcag cgggatcgcg ttcacgtacg agaactacgc cgcctgggag cgcctcgcgg
      301 gcggccgggg aacgctggac gatgtgcgct tcctggtcca cgagatggcg gaggtcggca
      361 tcctgcagca cgagggcgtg gacttcatgg gcgatcggta tacggtggat tcgccggccc
      421 acgaagactg gttcaagaga acgttcgagc cagcctacgc gcgcgcgcat gcgcaggcgc
      481 tgcaggtcga gttccagttc atctcggagc agctcgcgcg cgtgaccgag ggtcgcctgc
      541 agctctcacc cgcggagatc gcggcggtcg acacggtcaa cgcccaggcg cgcgagttcc
      601 tgctcgtcga cgggatgccg gtcgaccgac atccgcgcta ccgcgagtgg tttgggcgcg
      661 cgaccgcacc cgtcgagctc agcccggcga tgcggcagcg catcgggttg cccgagggtc
      721 gggacatcac gatcgagcag ctcgtgcgcg cgataaaaca ggcgcaggta acgggcggcg
      781 agttctcgcg ctcgtcgagc gagccgctat acttgggccc atcgctgcag ccaccgtgac
      841 accatgacgc cgcctgatcc cgatgaagtg acctacttcc tgaagcagct gtcctccgcc
      901 aacgcgttcg atcgcgcgat agcacttcgc tcgcaggcgg ggcggccgct ggcggatctg
      961 cgtctgctgg cccgcgccga ggagctgctc gccgatagct cccgtgtctg ctcgaaatcc
     1021 cctacctctt tggcgaggtg cggtggcgcg cgacgcaagc ggtggcggcg ctgcgggccg
     1081 cgctcggcaa gctcgagccg gtcgagctgc gcgacgtgcc ggtctcgctc agcgccaacg
     1141 agctatcgca gctggccctg cagcacggcg tgaccagcaa ccagtggggg aaggaggcga
     1201 tgctagacta cttcgcgcag ctgcgtgagc gcaacctgct gccgcgcggc gacgtcctgg
     1261 aggatccggc attctaccgc gatcacccgt gatggttcgg tccatcgcta tgacaccatg
     1321 aggccacccg atcccgacga tgtgaagttc ttcctgacgt ggctctcctc cgctgacgtg
     1381 caggagcgcg cgaccgcgct ccgctcgcag gccaaccgac cgcttgccga tccgcgccta
     1441 ctggcccgcg ccgaggagct gctcaacgac accaccctgt gccggctcga gatcccgctc
     1501 ctctacggcg agatacggtg gcgcgcagcg caggcagtgg cggcactgcg tgccgcactg
     1561 ggcaaggtcg agccagtgga gctgcgcgac gtgctcgttc cgctccgcgc taacaaagcg
     1621 tgccagctcg ccgaacagca cggcgcgacc agtgcccggg cggggaatga cggcgtgctg
     1681 gagtgcctcg cgcagctgcg cgagcgcggc ctgctaccgc gccgcgacat tgtggaggat
     1741 ccggcgttct accgcgcgcc cccgtgatgt tggcggcggg ccgagtgttt cctggtcgct
     1801 cactggccgc gatccgcatg cggatgggcc gttcggcgca tcgccgatgc cgcgcctggt
     1861 gcgcgcagat acacatcatc gtggccctgt tcgcgtagcg cgaccggcga tcgaccggat
     1921 gtggagccaa acgctgcgcg aacgcatttt tcgtgtcgcc tcgcaatcgg gtcgccgtcc
     1981 gggaaggcgg accgcctcga accgaacggg agcagcaatc cgttccggcg cccgggtgcg
     2041 gcccatgtgt ccgaccagcg aacccagtgc aaggagaaat gactcatgag aaccccatgg
     2101 accgtggctg cgctcttcgt atgtctgcag gcctgtgtca ccaacgtctc cgacgagcgc
     2161 tccggaccat cgccagacac cgcgtccacg acgggcgccg tgttatcggg gggcgatggc
     2221 gacccggatg tgccctgcat catcgacacc ggcccgggct gtgcgcccgg aaccagcatc
     2281 ggctccccgg cggggacctg cacgacgacc gagatcgcca atgcgcgcct caacggcgtc
     2341 aacttctgga cctgccagaa cggcgaccgc tacatctgcg atgatcacag ccacaagatc
     2401 cgggagacct gtatcaacgg ctgtgtctcg catcccgcgg gcgccgacga cgagtgcgcg
     2461 ttcggcagcg cggtccagtg cacgtcggcc gaattcagcg gccagaacct gctcgtgaac
     2521 gggttcaacg ccagcttctg gacctgccag ggcgccgccc gctacgtctg cgacggtgcc
     2581 tcggcccact acaaggtgaa ggagtcctgc cccaacggct gccagggcgc cggcgtcggt
     2641 cgcgacgacc agtgcctgtg acgccccccg ctcagcgcgc ggccatcgtg accaggtagt
     2701 cctggatcgc ctggcgctcg ctgaggtcga cgtgcgcgcg ccgcgacatc tcgtcgatca
     2761 tgtgcggcca ctcgtcggag cggtgctcgg ccggctgcgg cgtgcggtgg cagctgctgc
     2821 acttggcggc gaccagctgg cggccctgct ggagctcggc gagcgcgacc ccggagcggg
     2881 cggcatcccc ggcggtcacg accggcagcg cgcccccgca gccagcggac agagccaggg
     2941 acgcgagcac ggcgcggaac ctcatgacga gttgaaatta actttcaatt tccgccgcgt
     3001 caagcacgga aacctgatgg aggctggacc cggccggcat ttctacatgg aaatcgagcc
     3061 cttgcggaag tcggtcttgc gcaggtggac gttgatgcag accgggcgcc cggtgcgcgc
     3121 gatcgccttg gcctcggcca gcacgccgtc gacccgcgcg ccgtcggtca gcgccaggcc
     3181 gacgccgccg tagccctcgg cgaccttgtg gtaatcggtg cggcgcaggt cgcagccgag
     3241 cgtggtgccg agcacctcga cctgctcgcg ggcgatctgc atccacgacg cgtcgttgcc
     3301 gatcaccgcg atcggcgcga cgccgtgtcg cacgtagctg tcgaactcgg cgagggtgta
     3361 cgccgacgac ccgtcgcccc acacgagcca cacctccttg ccgggccgca ccatcgccgc
     3421 gccgagcgcg aacccgccgc cgaccccgag cgtgccgaac gcgcccgggt cgagccacga
     3481 cagcggcgcg cgcggccgga cgatgtagct cgcggtcgcc acgaagtcgc cgccgtcgac
     3541 caccagcacc gcgtcgtcgg ccatcgcctc ctcgaggcgg aggaagaagt g
//
LOCUS       DASVOR010000517         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_209327,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000517 DASVOR010000000
VERSION     DASVOR010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209327"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VF516_10340"
     CDS             complement(<1..194)
                     /locus_tag="VF516_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108116.1"
                     /translation="MQSASPGNAANGASPQSAVRTTRGARDPAIAEPVAEAALHPVDA
                     LRTNARGLAADLTLAGVGVGR"
     gene            complement(306..2369)
                     /locus_tag="VF516_10345"
     CDS             complement(306..2369)
                     /locus_tag="VF516_10345"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8108117.1"
                     /translation="MAGPTRREFLELAGALGASLALGCPRSAPLRWALRPDCFPQGVA
                     SGDPDEHSVLLWTRRPPVGGDAARRVTVEVAEDAGFAHVVASADAELSAASDWTARVL
                     AAGLAPARVYWYRFVDERGNGSRIGRTITAPAPGDPRPVQFAFVSCQNVCQGAQTAYR
                     RMIFEDERRAEADRLGFVLHLGDFFYELVWYPEDRPQGMYARRLRDVVRFSTGEKIRD
                     FHVPVTLDDYRALFRAYLQDPDLQDARARWPFVCMWDNHEFSWRGWQSLVKFDGARPA
                     QTRKVAAAQAWFEFQPARVADPAGRRITRFDAPAVTDAPIERFDDHGLGQEPNNLAAI
                     HALRLYRTLRWGAHLDLILTDNRSFQMEPVMDRREAEPFQSKPHRYFVPEDVIAVLDA
                     GRGFPGGPPATIRFGGRDLPNPRAQHPPQTALGVAQKAWFLERLERSTATWKVWGNSF
                     ATLDGRTDLHHLPAGLGPAWPGAGLASIGGTDWTGYRTERAEIFDFVRDRRLTGFAIV
                     SGDRHSFWAGRVSRSLPPAPFEPVGVEFVTGSVSAPGLIEAAEYGMAPDDPLRPLYVH
                     AAAPGAALQPTLNFLLLHGVRSCLEYVRTGDLPRALALSNPELAPHLAFLDLGGHGYA
                     VVRLDGGALETEFVCIPRPLERSDRPDGGPLLYRVAHRARLWRPGEPPQLERTRLEGT
                     PPLAT"
     gene            2633..>2971
                     /locus_tag="VF516_10350"
     CDS             2633..>2971
                     /locus_tag="VF516_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108118.1"
                     /translation="MIGRDACGVGFVADIKGARSREIVDDGLEVLRRLSHRAACGADP
                     ETGDGAGILIQLPERLFRAEAARLGFELAPNRRFAVGQVFLPPGREARAACEAILEQA
                     IAAEGQRVVGW"
BASE COUNT          408 a         1045 c         1133 g          385 t
ORIGIN      
        1 cgaccgacac ccacgccagc gagggtgagg tccgcggcaa gaccgcgagc gttcgtgcgc
       61 agcgcgtcga ccggatgaag cgccgcttct gcaaccggtt cggcgatggc ggggtcccgg
      121 gcgcctctgg ttgtgcgcac cgcggattgg ggcgacgccc cgttcgctgc attgccaggg
      181 ctcgccgact gcacggtggc cgtaacagcc tcccccgatg cccgccagtc gtaccccggg
      241 aacttgccct cgcgcgtggt ctcgattcga tgtcaccgtg aatcccggcg gctcacagca
      301 gcgggctacg tggcgagcgg cggcgtgccc tcgagccggg tgcgctccag ctgcggcggc
      361 tcgccggggc gccacagccg ggcgcggtgg gcgacccggt agagcagcgg gccgccgtct
      421 ggacggtcgc tgcgctccag cggccgcggg atgcacacga actcggtctc gagcgcgccg
      481 ccgtcgagcc ggaccaccgc gtagccgtgg ccgccgaggt cgaggaacgc aaggtgcggc
      541 gcgagctcgg ggttggacag cgcgagcgcg cgcggcaggt cgcccgtccg cacgtactcc
      601 aggcacgacc gcacgccgtg caggagcaaa aagttcagcg tgggctggag ggccgcgccc
      661 ggcgccgcgg cgtggacgta gagcgggcgc agcggatcgt cgggcgccat gccgtactcg
      721 gcggcctcga tcagaccggg tgccgagacc gagccggtga cgaactcgac gccgaccggc
      781 tcgaacggcg cgggcggcag cgagcgcgac acccggccgg cccagaacga gtggcgatcg
      841 ccgctgacga tcgcgaaccc ggtcagccgg cgatcgcgga cgaagtcgaa gatctcggcg
      901 cgctcggtgc ggtagccggt ccagtcggtg ccgccgatcg aggcgagccc ggcgcccggc
      961 cacgccggcc cgagcccggc cggcaggtgg tggaggtcgg tgcgcccgtc gagcgtggcg
     1021 aacgagttgc cccacacctt ccaggtcgcg gtcgagcgct cgaggcgctc gaggaaccac
     1081 gccttctgcg cgacgccgag ggcggtctgc ggcggatgct gcgcgcgcgg gttggggaga
     1141 tcgcggccgc cgaaccggat cgtcgccggc ggcccgcccg ggaagccacg gccggcgtcg
     1201 agcacggcga tcacgtcctc ggggacgaag tagcggtgcg gcttgctctg gaacggctcg
     1261 gcctcgcggc gatccatcac cggctccatc tggaacgagc ggttgtcggt gaggatcaga
     1321 tcgaggtgag cgccccagcg cagggtgcgg tacagccgga gcgcgtggat cgcggccagg
     1381 ttgttgggct cctggccgag gccgtggtcg tcgaaccgct cgatcggcgc gtcggtgacg
     1441 gccggggcgt cgaaccgcgt gatccgccgg ccggccggat cggcgacccg cgccggctgg
     1501 aactcgaacc acgcctgggc ggcggcgacc ttgcgggtct gggccgggcg cgctccgtcg
     1561 aacttgacca ggctctgcca gcctcgccac gagaactcgt ggttgtccca catgcagacg
     1621 aacggccagc gcgcccgcgc gtcctgcaga tccggatcct ggagataggc gcggaacagc
     1681 gcgcggtagt cgtcgagcgt gaccgggacg tggaagtcgc ggatcttctc gccggtcgag
     1741 aaccgcacga cgtcgcgcag ccggcgcgcg tacatgccct gcgggcggtc ctcggggtac
     1801 cacacgagct cgtagaagaa gtcgccgagg tggagcacga agccgagccg gtcggcctcg
     1861 gcgcgccgct cgtcctcgaa gatcatccgc cggtaggcgg tctgggcgcc ctggcagacg
     1921 ttctggcagc tgacgaacgc gaactggacc ggccgcggat cgcccggcgc cggcgcggtg
     1981 atcgtgcggc cgatccggct gccgttgccg cgctcgtcga cgaaccggta ccagtacacc
     2041 cgcgccgggg ccaggccggc ggcgagcacg cgcgccgtcc agtcgctggc cgccgacagc
     2101 tcggcgtccg ccgaggcgac cacgtgcgcg aacccggcgt cctccgcgac ctcgaccgtc
     2161 acgcggcgcg cggcgtcgcc gccgaccggc gggcggcggg tccacagcag cacgctgtgc
     2221 tcgtcgggat cgcccgatgc gacgccctga ggaaagcagt cgggccgcag cgcccagcgc
     2281 agtggcgcgc tgcgcggaca gccgagcgcg agcgaggcgc cgagggcccc ggcgagctcc
     2341 agaaattctc gtcgcgtcgg tccggccatg gctggagatc gcacggcgac gcgaggcgcg
     2401 gtcgctgtac gggtgcgcgt gcccattccc tgtgcgggac tcccgctcac cgacgacggc
     2461 gtccgccccg ggcggcgaag cacagcccct gtgcaattgc gtcaaccgac cagtcggaaa
     2521 cggtgcgacg cgtcattttg tactagctct tggtaacgcg acgcattaca gtgggcctgt
     2581 ttttccggga gatgccatgt cgccaacttc ctggaccggg ctcatcgacc ccatgatcgg
     2641 ccgtgacgcg tgcggcgtcg gcttcgtcgc cgacatcaag ggcgcgcgct cgcgcgagat
     2701 cgtcgacgac ggactcgagg tcctgcgccg gctgtcgcat cgcgcggcct gtggggcgga
     2761 tccggagacc ggcgacggcg ccgggatcct gatccagctg cccgagcggc tgttccgcgc
     2821 cgaggccgcg cgcctgggct tcgagctcgc gccgaaccgg cggttcgcgg tcggccaggt
     2881 gttcctgccg cccggtcggg aggcgcgcgc cgcgtgcgag gcgatcctcg agcaggcgat
     2941 cgccgccgag ggccagcgcg tggtgggctg g
//
LOCUS       DASVOR010000518         8713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_267550,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000518 DASVOR010000000
VERSION     DASVOR010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8713
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267550"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1552)
                     /locus_tag="VF516_10355"
     CDS             complement(23..1552)
                     /locus_tag="VF516_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8108119.1"
                     /translation="MATLLPAGEVGRPYTFHEAVYIAGAWRDGRGTRREQDRDPYTGE
                     VLVELQLAGHADLDEAYRAAATAGPRWAAAPPHDRSEVLRRAATIMVARRDEIVRWLV
                     REAGSTRIKATLEWDAVHAVIEDAAMMPYQVEGGMKRVDIPGKESRIYRKPVGVVAVI
                     SPWNWPLQLTARSVFPALAVGNAVVVKPASDTPITGGLLVGKILEEAGLPPGVINVLV
                     GSGSEIGDDVVRHPVPRVISFTGSTPVGRGIARLAAEATIIKRVELELGGNAPFVVLD
                     DADLERAAEAAVFGKFLHQGQICMIANRFIVDEAVYDEFLDRFVARVKALKVGNPDEP
                     DTMIGPIINEKQLRGLQHRISQARAAGFRELAGGAASGLVLPPHIFADVTNNHPVAKE
                     ELFGPIAPVIRAYGERDALRLANETEYGLSSCVFTRDLERGVEFAQCLEAGMAHVNDQ
                     PVNDIPGNPFGGEKNSGIGRFNGAWAIQAFTTDQWVTVQHAPRAYPTSADQIAGAWGG
                     G"
     gene            2160..2756
                     /locus_tag="VF516_10360"
     CDS             2160..2756
                     /locus_tag="VF516_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8108120.1"
                     /translation="MSKSDPGVEPARIARQPARATAPAGAATRRMSLKVNGEVFDLDL
                     DVRTTLLDALREHLHLTGTKKGCDHGQCGACTVLIDGRRLNACLTLAVMHEGDDIVTI
                     EGLGAPEALHPMQAAFLEHDGFQCGYCTPGQICSAIGMLSEVRAGWPSHVTADVASRQ
                     IELTDEELRERMSGNLCRCSAYPNIVAAIRDAVGRGRA"
     gene            2753..3745
                     /locus_tag="VF516_10365"
     CDS             2753..3745
                     /locus_tag="VF516_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX8108121.1"
                     /translation="MRPFTYERATDPAAAVAAAAAAGDGAKFISGGTNLLDLMKLQIE
                     RPSHLVDISRIPLRQIEDTADGGLRIGAQADNATVAADDRVRRRYPVVAQALLSGASA
                     QLRNKASTGGNLLQRTRCVYFYDTTAACNKRNPGAGCAALHGANRNHAILGASDACIA
                     THPSDMAVAMIALDARVELLGAGGATREVALRDFHLLPETTPHLETVLGPGELITAVV
                     LPAPPRGRQLYRKVRDRASYEFALVSVAAIVSVAGASIETARIAFGGVAHKPWRSLEA
                     EAVLTGRAATLDTFRTAADAALARAVGRGRNDFKIDLARRTLVAALATLTEARP"
     gene            3742..5901
                     /locus_tag="VF516_10370"
     CDS             3742..5901
                     /locus_tag="VF516_10370"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX8108122.1"
                     /translation="MIGPPLSRVEGPRKVTGRATYTYEEWGAGPPLYGFIVEAAIGRG
                     RIARLDTSRADRARGVRAVITHRNAPRQNQPDPSVASPYSRALPMLTESEIRYFAQPV
                     ALVVAEAFEQARDAAGLVDAAYEPSEGRFDLAARLDDAHAPPTVRGWIEPDSLVGDFD
                     RAFAAAPIRIDETYTTPYEFSAPMEPHCALAVWHGDAVTVYAATQIVAEAKLRIAATL
                     GLAPAQVRVIAHYIGGGFGSKLGVHAETILAVIAARQLGRPVKVMATRQQTFPLAGHR
                     PATVQRVRLGADRDGRLIAFGHDPIQQASFTSDFVEQIGSVGRALYAALHRRSTHRIV
                     PLDLPGGEDVRAPGEAPGLLAIEAAMDELAYALDLDPVELRIRNEPAQHPELGVPYSD
                     RHLVECLREGARRFGWERRLKRPATLRDGRWLVGHGMAAAIRPQFQARTSVTVRLEPD
                     GTAIVRTDMTDLGTGTYTIATQVASEALGVPIDRVRVEAGDSEFPVSGGSGGSWGATN
                     TCTALLRACHALRDRIFASMPEAERASAGGLPALVAARFPDGVEASGAIPDMSDDPRF
                     TAHSLSSYGAHFAEVRVDVDTGEIRLRRMLGVFDAGRIFNARTARSQLIGGMIWGVSA
                     ALHEDGAVDPRTGAFVNRDFAQYLIPVHADIPAIDAILLDTYDPHANELGAKGVGEIG
                     ICGAGAAVANAVYNATGVRVRDYPITLEKLLPHLPRP"
     gene            6478..7311
                     /locus_tag="VF516_10375"
     CDS             6478..7311
                     /locus_tag="VF516_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8108123.1"
                     /translation="MATVRTVKLPAGEEVPALGLGTWHLGEDPAKRREEIAAIRTGLD
                     LGMTLIDTAEMYVGAEDLIREAVAGRRNECFIVDKLLPTHATRRGTVEACEKSLRRLV
                     TDHIDLYMLHWRGSAPLWDTVEAFDELVATGKIRYWGVSNFDVDDMNELFQVPDVDPQ
                     ADQVLYNLTHRGIEWDLLRWCRTRNIPIMAYSPIEQGALLDDPALKRVAKRHGATSAQ
                     IAIAWVLRHPDVMTIPKAGTPEHVRENRGALDIELSSKDLRDLDDVFEAPSNKVPLEM
                     I"
     gene            7391..8356
                     /locus_tag="VF516_10380"
     CDS             7391..8356
                     /locus_tag="VF516_10380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8108124.1"
                     /translation="MPETMTAAAIDRFGPPDVLTPHTLPVPKVGQTEIVISLHAAGVG
                     IWDAKIRDGTWAPEGVEFPLVLGTDGAGIVAAKGPRAQGFEVGDRVWGYSYMNPKGGF
                     YADYVVVEASHVGHVPLQLDLVHTGMSVVTALTALQGLDEHLHVRRTERVLIFGASGA
                     VGTFAVQLAKWRGAFVIATARGKDAQELVRQLGADAVIDPQTPSGLARLEQLAPHDID
                     AVLALTGGVELERCLDHVRRGGRVAYPDGVEPRPAHRAGLDVIAYDAVPGPREYERLA
                     HAFLDAHLRAVISAEFPLERAADAHRRLEQGHVLGRIGLLIATGR"
     gene            8373..>8713
                     /locus_tag="VF516_10385"
     CDS             8373..>8713
                     /locus_tag="VF516_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108125.1"
                     /translation="MAAVTAPSEQPASEMLFRPIRVGPFTLPHRIVMAPLTRSRARTP
                     GNVPTELNACYYAQRASAALIISEATQVSMQGQGYAWTPGIHSREQVEGWRVITDAVH
                     DAGGRMFIQLW"
BASE COUNT         1269 a         3164 c         3014 g         1266 t
ORIGIN      
        1 cggagatcct cgaccgcatc cgtcagccgc cgccccacgc gccggcgatc tgatcggcgc
       61 tcgtcgggta cgcgcgcggc gcgtgctgca cggtcaccca ctggtcggtc gtgaacgcct
      121 ggatcgccca cgcgccgttg aaccggccga tgccggagtt cttctcgccg ccgaacgggt
      181 tgcccgggat gtcgttgacc ggctgatcgt tgacgtgcgc catgcccgcc tcgaggcact
      241 gcgcgaactc cacaccgcgc tcgaggtcgc gcgtgaacac gcagctcgat agcccgtact
      301 cggtctcgtt cgcgaggcgc agcgcatcgc gctcgccgta cgcgcggatc accggcgcga
      361 tcgggccgaa cagctcttcc ttcgcgaccg ggtggttgtt cgtgacatcg gcgaagatgt
      421 gcggcggcag cacgagcccg ctcgcggcgc cgccggcgag ctcgcggaac ccggccgcgc
      481 gcgcctgcga gatccggtgc tgcagcccgc gcagctgctt ctcgttgatg atcgggccga
      541 tcatcgtgtc gggctcgtcg gggttgccga ccttcagtgc cttcacccgc gcgacgaagc
      601 gatcgaggaa ctcgtcgtag acggcctcgt cgacgatgaa ccggttcgcg atcatgcaga
      661 tctggccctg gtgcaggaac ttgccgaaca ccgcggcctc cgcggcgcgt tccagatccg
      721 catcgtcgag cacgacgaac ggcgcgttgc cgccgagctc gagctccacg cgcttgatga
      781 tcgtcgcctc cgccgcgagc cgcgcgatgc cgcgccccac cggcgtcgag ccggtgaacg
      841 agatcacgcg cggcaccggg tggcgcacga cgtcgtcgcc gatctcgctg cccgacccga
      901 cgaggacatt gatcacgccc ggcggcaggc cggcctcctc gaggatcttg ccgacgagca
      961 gcccgccggt gatcggcgtg tcgctcgccg gcttcacgac gaccgcgttc ccgacggcga
     1021 gcgccggaaa caccgagcgc gcggtgagct gcagcggcca gttccacggg ctgatcacgg
     1081 cgacgacgcc gacgggcttg cggtagatcc ggctctcctt gccggggatg tcgacgcgct
     1141 tcatgccgcc ctcgacctgg tacggcatca tcgcggcgtc ctcgatcact gcgtgcaccg
     1201 cgtcccactc gagcgtcgcc ttgatccgcg tgctgccggc ctcgcgcacg agccagcgca
     1261 cgatctcgtc cctgcgcgcg accatgatcg tggcggcgcg gcgcagcacc tcggagcggt
     1321 cgtgcggcgg cgcggcggcc cagcgaggtc ccgcggtagc ggcggcgcga tacgcctcgt
     1381 cgaggtcggc gtgcccggcg agctgtagct cgacgagcac ctcgcccgtg tacggatcgc
     1441 gatcctgctc gcgacgcgtg ccacggccgt cgcgccacgc ccccgcgatg tagaccgcct
     1501 cgtgaaatgt gtagggtctg ccgacctcgc cggctggaag caatgtcgcc atgcaccgta
     1561 tttacccgtg ccgatcgccg ctggccatcg cgcgcgtgcg cactccgata tgctcgcggc
     1621 gatgacgcgc gtgaacgtcc cgctgcgcta cgcctaccgc ttgctcgacc acggtccgac
     1681 gacgctcgtc accacgcgcg caggcgatcg tacgaacctc atgtcggcgc agtgggtgat
     1741 gccgctcgac tttcaccacc aaggacgtcg gcgagcaccg gcctcggcct gtcgatcgtc
     1801 cacgagctcg tcgagcgcca cggcggcacg atcgagtgcc acaccacggt cggcgccgtc
     1861 accacgttca ccgtcaagag ctgccgcgcc agatcccggt caccgccaag cccgcagcgc
     1921 gaagcccacc agcccgccgg tgtcgcgctc ggtctgagcg cgaccctcgg cgccgagcca
     1981 tcgaccgcca ggattccctg cgacgctgtt acccgctcgc gcgggccggg ccgggccgat
     2041 ccggtgcgcc gtgcgggcac gctggtgcgg atgagcgctc ggcgtttccc ctcgacggcg
     2101 cggctccgac gaggcgtgcc gtccggcgcg tggttcgtcc gttgcaccga ccgtcggtca
     2161 tgtccaagtc cgatcccggt gtggagcccg cgcggatcgc gcgacagccg gcgcgcgcca
     2221 cggcgccggc aggagctgcg acccgcagga tgtcgctcaa ggtcaacggc gaggtgttcg
     2281 acctcgatct cgatgtcagg acgacgctgc tcgatgcgct gcgcgagcac ctccacctga
     2341 ccgggaccaa gaagggttgc gatcacgggc aatgcggcgc ctgcaccgtg ctgatcgacg
     2401 gccggcggct caacgcgtgc ctcacgctcg ccgtcatgca cgagggtgat gacatcgtga
     2461 cgatcgaggg gctcggcgca ccggaggcgc tccatccgat gcaggcggcg ttcctcgagc
     2521 acgatgggtt ccagtgcggg tactgcacgc cgggccagat ctgctcggcg atcggcatgc
     2581 tgagcgaggt ccgggcgggc tggccgagcc acgtcaccgc ggacgtcgcg tcgcgccaga
     2641 tcgagctgac tgacgaggag ctgcgcgagc ggatgagcgg caatctgtgc cggtgctcgg
     2701 cgtaccccaa catcgtcgcg gcgatccgcg acgcggtcgg ccgggggcgc gcatgaggcc
     2761 gttcacctac gagcgcgcga ccgatccggc cgcggcggtg gccgctgccg cggcggcggg
     2821 cgacggcgcg aagttcatca gcggtggcac gaacctgctc gatctgatga agctgcagat
     2881 cgagcggccg tcgcacctgg tggacatcag ccggatcccg ctgcgccaga tcgaggacac
     2941 ggcggacggc gggctgcgga tcggcgcgca ggccgacaac gccaccgtgg ccgccgacga
     3001 tcgcgtgcgc cggcgctacc cggtcgtggc gcaggcgctg ctgtccggcg cgtccgcgca
     3061 gctgcgcaac aaagcctcga ccggcggcaa cctgttgcag cggacgcgct gcgtgtactt
     3121 ctacgacacc accgcggcgt gcaacaagcg caaccccggc gcgggctgcg ccgcccttca
     3181 cggcgccaac cgcaaccacg cgatcctcgg cgcgagcgac gcgtgcatcg cgacccatcc
     3241 atcggacatg gcggtcgcga tgatcgcgct cgacgcccgg gtcgagttgc tcggcgccgg
     3301 cggagcgacg cgcgaggtcg cgctgcgcga cttccacctg ctgcccgaga ccacgccgca
     3361 cctcgagacc gtgctcgggc ctggcgagct gatcacggcc gtggtgctgc cggcgccgcc
     3421 gcgtggccgc cagctgtatc gcaaggtccg cgatcgagcg tcctacgagt tcgcgctggt
     3481 ctcggtcgcc gcgatcgtgt cggtggccgg cgcgtcgatc gagaccgcgc ggatcgcgtt
     3541 cggcggcgtc gcgcacaagc cgtggcgctc gctcgaggcc gaggcggtcc tgaccggccg
     3601 cgctgcgacg ctcgacacgt ttcgcaccgc cgcggatgcc gcgctcgcgc gcgccgtcgg
     3661 ccgcggccgc aacgacttca agatcgatct cgcccggcga acgctggtcg cggccctggc
     3721 gacactcacg gaggcgcggc catgatcgga ccgccgctca gccgcgtcga gggcccgcgc
     3781 aaggtcaccg gccgcgcgac gtacacctac gaggagtggg gcgccggacc gccgctctat
     3841 ggcttcatcg tcgaggcggc gatcgggcgc gggcggatcg cgaggctcga cacgtcgcgc
     3901 gccgatcggg cgcggggtgt gcgcgctgtg atcacgcacc gcaacgcgcc ccgccagaac
     3961 cagcccgatc cgtcggtggc gtcaccctac tcgcgcgcgc tgccgatgct caccgaatcc
     4021 gagatccggt acttcgcgca gccggtcgcg ctcgtcgtcg ccgaggcgtt cgagcaggcg
     4081 cgcgacgcgg cggggctcgt cgacgccgcc tacgagccga gcgaggggcg gttcgacctc
     4141 gccgcgcggc tcgacgacgc ccacgccccg cccaccgtcc ggggctggat cgagcccgat
     4201 agcctcgtcg gcgacttcga ccgcgcgttc gccgcggcgc cgatccggat cgacgagacc
     4261 tacaccacgc cgtacgagtt cagcgcgccg atggagccgc actgcgccct cgcggtctgg
     4321 cacggcgatg ccgtcacggt gtacgcggcc acgcagatcg tcgccgaggc gaagctgcgg
     4381 atcgcggcga cgctcgggct cgccccggcg caggtccggg tcatcgcgca ctacatcggc
     4441 ggtgggttcg gctcgaagct cggcgtccac gccgagacga tcctcgccgt gatcgccgcg
     4501 cgccagctcg gccggccggt caaggtgatg gcgacccggc agcagacgtt cccgctcgcc
     4561 gggcaccggc cggccacggt ccagcgcgtg cggctcggcg ccgatcgcga cggccgcctg
     4621 atcgcgttcg gtcacgatcc gatccagcag gcgagcttca cttccgattt cgtcgagcag
     4681 atcggctcgg tcggccgggc gctctacgcc gcgctgcacc gccgctcgac ccatcgcatc
     4741 gtcccgctcg atctcccggg cggcgaagac gtccgcgctc ccggcgaggc tcccggcttg
     4801 ctcgcgatcg aggcggcgat ggacgagctg gcgtacgcgc tcgacctcga ccccgtcgag
     4861 ctgcggatcc gcaacgagcc ggcgcagcat cccgagctcg gtgtcccgta cagcgatcgc
     4921 cacctggtcg agtgcctgcg cgagggcgcc cggcggttcg ggtgggagcg ccgcctgaag
     4981 cgccccgcga cgctgcgcga cggccgctgg ctcgtcggcc atggcatggc ggccgcgatc
     5041 cgtccccagt tccaggcccg cacctcggtc acggtccggc tcgagccgga tggcacggcg
     5101 atcgtgcgca ccgacatgac cgacctcggc acgggcacat acacgatcgc gacccaggtc
     5161 gcgagcgagg ccctcggcgt ccccatcgat cgcgtgcgcg tcgaggccgg tgactcggag
     5221 ttcccggtca gcggcggctc gggcggatcg tggggtgcga cgaacacctg cacggcgctg
     5281 ctgcgcgcgt gtcacgcgtt gcgcgatcgg atcttcgcgt cgatgcccga ggccgagcgc
     5341 gcgagcgcgg gcggtcttcc tgcgctcgtc gcggcgcgct tcccggacgg cgtcgaggcc
     5401 agcggcgcga tccccgacat gagcgacgat ccgaggttca cggcccactc gctgtcgagc
     5461 tacggcgcgc acttcgcgga ggtgcgggtc gacgtcgaca ccggcgagat ccggctgcgc
     5521 cggatgctgg gcgtgttcga cgccgggcgg atcttcaacg ccaggaccgc gcgctcgcag
     5581 ctgatcggcg ggatgatctg gggcgtcagc gccgcgctcc acgaggacgg tgccgtcgac
     5641 ccgcgcaccg gcgcgttcgt gaaccgcgac ttcgcgcagt acctgatccc ggtccacgcc
     5701 gacatccctg cgatcgacgc gatcctcctc gacacctacg atccccatgc caacgagctc
     5761 ggcgccaagg gcgtcggcga gatcggcatc tgcggcgcgg gtgctgcggt cgccaacgcg
     5821 gtctacaacg cgaccggcgt ccgcgtgcgc gactatccga tcacgctcga gaagctcctg
     5881 ccgcacctgc cgcgcccgta gcgcccggcg gcgccgaccg gcatgcccga ccgcggtgcg
     5941 accgcggtca caccaccggg ccgcgaacat tcgtgcgact tgtcgcccga gcctgctctg
     6001 gatccccagg gatcgtcgaa gcgacatgct ggaagcgcgc accgaaccgc cactccatct
     6061 ccacgtgacc gacaccacgc tccaggggac tgacaccacg gtgtccatcg cgcccgtgcg
     6121 aggtccacat caacgacttc tacttctggc cgccctgaag gggtattgcg gacgggcgca
     6181 gctggaccaa tcacacgacc gcggtttgca cccgtgacgc acccgggtgc atcgccaacg
     6241 tcttcggccc ggcccgaagg cgcaggcgcc agctccccac cacgtctctg accggcgacc
     6301 gcatgcccgt ggaccgcgcc ggcgcgcggc aatacctcgc ggccagtgcc cgcgcgcggc
     6361 tggcggtcgt cgtgccaccc cgcgcgtcgt cgtgcagccg agcaagttgc tccgatgata
     6421 gttgctcggc acgacgcatg cttttggttg gatgtgcagc agacctgacg aggcatcatg
     6481 gcgacagttc gtacagtcaa gctcccagcg ggcgaagagg tgcccgcgct cggcctgggc
     6541 acctggcatc tcggcgaaga tccggcgaag cggcgcgagg agatcgccgc gatccgcacg
     6601 ggcctcgatc tcgggatgac cttgatcgac acggccgaga tgtacgtcgg cgccgaggat
     6661 ctgatccgcg aggccgtcgc cgggcggcgc aacgaatgct tcatcgtcga caagctgttg
     6721 ccgacgcacg cgacgcgacg cgggaccgtc gaggcctgcg agaaaagcct tcgccggctc
     6781 gtcaccgacc acatcgatct gtacatgctg cactggcgcg ggtcggcgcc gctctgggac
     6841 accgtcgagg cattcgacga gctggtcgcg accgggaaga ttcgctactg gggcgtcagc
     6901 aacttcgacg tcgacgacat gaacgagctg ttccaggtcc ccgatgtcga tccgcaggcc
     6961 gaccaggtgc tctacaacct cacgcatcgc ggcatcgagt gggatctgct gcggtggtgc
     7021 cgcacccgga acattccaat catggcgtac tcgccgatcg aacaaggcgc gttgctcgac
     7081 gatccggcgc tgaagcgcgt cgccaagcgg cacggcgcga catcggcgca gatcgcgatc
     7141 gcgtgggtgc tccggcatcc cgacgtcatg acgatcccga aagccggcac gcccgagcac
     7201 gtgcgggaga atcgcggagc cctcgacatc gagctgtcgt cgaaggacct gcgcgatctc
     7261 gacgacgtgt tcgaggcgcc gtcgaacaag gtgccgctcg aaatgatttg aggacaggtg
     7321 aaccatgcca atcgccgaga aacgacccag cagagagcgc gagacggcgc cgcatcgcta
     7381 tccgcgcgcc gtgccagaga cgatgacggc ggccgcgatc gatcggttcg gcccaccgga
     7441 tgttctgacg ccacatacgc tgccggtacc caaggtcggg cagaccgaga tcgtgatctc
     7501 gctccacgcc gccggcgtcg ggatctggga tgccaagatc cgcgacggca cgtgggcgcc
     7561 cgagggcgtc gagttcccgc tcgtgctcgg caccgatgga gccggcatcg tcgcggcgaa
     7621 gggcccgcgc gcacagggct tcgaggtcgg cgaccgcgtc tggggttact cgtacatgaa
     7681 tcccaaggga ggcttctacg ccgactacgt cgtcgtcgag gcgagccacg tcggtcacgt
     7741 gccgctgcag ctcgatctcg tgcacaccgg gatgtcggtg gtgaccgcgc tgaccgccct
     7801 gcagggcctt gacgagcacc tgcacgttcg ccgcaccgag cgcgtgctga tcttcggcgc
     7861 ctcgggcgcg gtcggtacat tcgcggtcca gctcgcgaag tggcgcggcg cgttcgtgat
     7921 cgcgaccgcg cggggcaagg atgcccagga gctcgtgcgc cagctcggcg cggatgccgt
     7981 gatcgacccg cagacgccga gcggtctggc gcgcctcgag cagctcgcgc cgcacgacat
     8041 cgacgccgtg ctcgcgctca cgggcggcgt cgagctcgag cgatgtctcg atcatgttcg
     8101 ccgtggcggg cgcgtcgcgt atcccgatgg cgtcgagcct cggccggcgc atcgtgcggg
     8161 tctcgatgtc attgcatacg acgcggtgcc gggcccgcgc gagtacgagc ggctcgcgca
     8221 cgcgttcctc gatgcgcacc tccgggcggt gatctccgcc gagtttccgc tggagcgtgc
     8281 ggccgatgcg catcgccgcc tcgagcaagg gcacgtgctc ggcaggatcg gcctgctgat
     8341 cgccacaggt cgctagccag ctggagatca cgatggccgc cgtcacagct ccgagcgaac
     8401 agcccgccag cgagatgctg ttccgcccga tccgggtcgg accgttcacg ttgcctcacc
     8461 ggatcgtgat ggcgccgctc acgcgctcgc gcgcacgcac gccggggaac gtgccgaccg
     8521 agctcaacgc ctgctactac gcccagcgcg cgtccgcggc gctcatcatc agcgaggcga
     8581 cgcaggtgtc gatgcagggc cagggctatg cgtggacgcc cggcatccac agtcgcgagc
     8641 aggtcgaagg ctggcgcgtg atcaccgatg ccgtccacga cgcgggcggt cgcatgttca
     8701 tccagctgtg gca
//
LOCUS       DASVOR010000519        10752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_472417,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000519 DASVOR010000000
VERSION     DASVOR010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472417"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VF516_10390"
     CDS             complement(<1..1430)
                     /locus_tag="VF516_10390"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEX8108126.1"
                     /translation="MTKHAAILCLVAVAAPAAADDHAPSSLQMRAPPPAGDEGAPPAE
                     DNGKTEVISVTDTPIERTLVTGRAPVSVVTRADLDASGRATLGDILQALPAQANAGNA
                     QVNAGGDGTTRINLRGLGAPRTLVLVNGRRMVNGGSGADASVDVNTIPLAMVERVEIL
                     KDGASALYGADAVGGVVNIITRPQFDGLDVSLLTSTSQHGDGTEYDASFVTGFTTKDK
                     NTYLVVSGGYQHHEPVLAGDRAFSQFQDSYDFASRTVTRNQSLAAIGGRLDPSSIGPG
                     GMAPPGCASDACKPDGNGGWTRFSSGDLYNEATQSYVYTPSTRYNVFGTAGNRLGDHA
                     ALLLEVSYLHRSSDRQLSPVAFTADSPISKDSLYNPLGGDLLDYRRRMTELGAREFID
                     TVSTTRIVLGVTGSVPASWGVLDGWKYELSYNYGTSDALAGTTGQLNKLRLTDALGPS
                     MLDAGGAPICVRVPGDATTKIVYHVFL"
     gene            complement(1610..1996)
                     /locus_tag="VF516_10395"
     CDS             complement(1610..1996)
                     /locus_tag="VF516_10395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108127.1"
                     /translation="MTAHPPCRAILTPSGDAEYGIRFELTNPTPRPIALDIDEPFLQF
                     RVRAAADGAALAVVQPTLDIPIHPTTVTVPAAGALLLASPVRLRFGAPSRDGLVWSIA
                     HAPAGVELTFTLDLPAPFDQPFTARL"
     gene            complement(2125..3423)
                     /locus_tag="VF516_10400"
     CDS             complement(2125..3423)
                     /locus_tag="VF516_10400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108128.1"
                     /translation="MADLGQASKPRISPVWPPLARQEGALAVRVSWEQYLRGEAMFRE
                     PAKSDHQGVGGAAARHDSASPGKRTLTSGLSSQPLVDGPTEVPATSTAAAPPRSGGPG
                     LVGLFGRPGASATPISDREPDGPRSTEVAQATTEGPAPEQATTQGAPSGSETPRPGPH
                     TPGAVSDAPTASYIVPFDRSPLAAPGERIICRAEFTGGTASEYEIVYTTTGGHFTTAT
                     GPTTCTIQGLTSGNVNFFVPTPWNGTDAVSITMQLKKKSDGSVVQTETWNFGKKAYYP
                     TTMTQREGTGEVDLPGRYTYDIGPARPSGTAPFYEHQTILEWFDNWSISNIAPEDIRE
                     AYRTAHGLTSTAAITTHFIGAYAGNNGTFTVNANDRVGDRHGGNPDVENLASNLTAPK
                     DIEVALPQTYEAQPGTALARFMVTRVRKTDGSWKVKKAPR"
     gene            complement(3597..4187)
                     /locus_tag="VF516_10405"
     CDS             complement(3597..4187)
                     /locus_tag="VF516_10405"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8108129.1"
                     /translation="MSTTKSSPRAPTLADLDALPPGIVGEIIEGVLYTMTKPRARHQR
                     TTRTIGGRVGDPFDHGRGGPGGWWILTEPGIELPNTPEISPDVAGWRRERMPELPADE
                     PICLVPDWVCEILSPTTRRHDMLRKQPYYAKVGVPFLWLVDIEARVLTVYRLESSDWR
                     MIGTYSDETEARIAPFDAVPLSVADWWPPAAPENST"
     gene            4633..4938
                     /locus_tag="VF516_10410"
     CDS             4633..4938
                     /locus_tag="VF516_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108130.1"
                     /translation="MPSAARVVVPPLASRAHELDRIIVEYAGDAIAELGAPHEAFTAD
                     DRAWVRDHAAGSLAEIEKATLRLVTLYTSRNLSHAAARLGMAPVSLSRWLGRRELPR"
     gene            4935..5252
                     /locus_tag="VF516_10415"
     CDS             4935..5252
                     /locus_tag="VF516_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108131.1"
                     /translation="MTPSDHGSRVNGSQVAEPLDDLRIALSEVDVLTTLTKAAYDDAD
                     WSGADPAVVDAMATLLGLIDKSASSAVAAFHRLHGAIADAQPAPAGERWDTEGRGTKP
                     GEG"
     gene            5249..5587
                     /locus_tag="VF516_10420"
     CDS             5249..5587
                     /locus_tag="VF516_10420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108132.1"
                     /translation="MTTRKPITLSLSVDSVEQLQRIGAAFFDEPKPKQPLFPFTDGNC
                     LARHESSVVSRLRKRLAEVYGKPVDHRFFEDQYLPDEHPNAALLRLFRQRQMLHMTDA
                     ETIESLAKGR"
     gene            complement(5667..6125)
                     /locus_tag="VF516_10425"
     CDS             complement(5667..6125)
                     /locus_tag="VF516_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108133.1"
                     /translation="MAAIEAAIANHPVAGLLIAPVVLLVCFGALCGMAPFGLIVGMVV
                     GIDPAWQERLGWLRWLGWGPWGGRLVFMGLTAGLIALVIAGRWLEKRNPVPNGDDDTP
                     RVPSARAVTAHLFTTVPWKQLIGPILLVIAGAGFLCLGCFGLAMSGMLDA"
     gene            6375..6617
                     /locus_tag="VF516_10430"
     CDS             6375..6617
                     /locus_tag="VF516_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108134.1"
                     /translation="MTAHPLHPLAAARWLTSAKLDAAEAVRGGAAVLTVGEDIAVAQR
                     AQAIAATDHRALIQAQRELAAAGAEIKLLLRRRPST"
     gene            complement(6689..7324)
                     /locus_tag="VF516_10435"
     CDS             complement(6689..7324)
                     /locus_tag="VF516_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108135.1"
                     /translation="MIQDVLAGVFVLHGFGARSEAVLRAAIARYRGGDFGGIDYMPPN
                     AAGEAWLLLGTWVDCFAPEVQQCIASFAHELQQVAELDRAGVEIGPQGSFILDPSRAF
                     PDSVRGELRALRAYRGGIAIRELGTNTQALIKRVLPELPAYQGVHMGAPHWLGRGVVQ
                     QGGRIHSVTPYIRIDFDDGHSIRIAAMAERRDFDPWLAALRAAILQMQGKN"
     gene            7652..8926
                     /locus_tag="VF516_10440"
     CDS             7652..8926
                     /locus_tag="VF516_10440"
                     /inference="COORDINATES: protein motif:HMM:NF017850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 family protein"
                     /protein_id="HEX8108136.1"
                     /translation="MDPLLNPHVDPRKLLALKSSMTLHDWETQIRGKMLQLPDRVLYT
                     WDRAVNERSVPDLGRITREFLTAWEGDRTRWDQLIAVDVQRFPWVAVGIFDVYRDPRT
                     PGDAKTRLLWMVDEIALSAGDEVDVCRELAAKGYDGERCLVVTDASCEWQQLERDLIR
                     QRPNYQGKGTNYILRENGFPHVVPPDRSAKANPGILERIRATNACIREGRAILGGKAT
                     IEVLNQALRSGSGAGDHLHPGIVSLSNSEISKAITAGTLTSDTGGPGSFALGQVHADR
                     AHKLSLADARRIGNVFRRDLGREYQAISELAERDRRPLTMWVRQRLEDAVAAARAAST
                     TGAGTRSGEPPEASLAHRGRWQAHRGVPCRSSDTSRYAGSRGSAATTAREAAEIEEPF
                     GYGRAGRPSRRGASRLRGRRRGDTAMSLLNCW"
     gene            complement(8948..9541)
                     /locus_tag="VF516_10445"
     CDS             complement(8948..9541)
                     /locus_tag="VF516_10445"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEX8108137.1"
                     /translation="MAFTLPELPWAKEALAPSISAETIEYHYGKHHKAYVDNLNKFAP
                     STKYESMSLEDVIKASQGQASEKKIFNNAAQVWNHTFFWHSLAPKAGGNPTGAIAAAI
                     DKSFGNFADFKTKFSEAAAGQFGSGWAWLVKNADGSLAIETTSNADTPFAVGKKCVLT
                     LDVWEHAYYLDYRNARPKFIEAFWNIVNWDFANKNIA"
     gene            9621..10553
                     /locus_tag="VF516_10450"
     CDS             9621..10553
                     /locus_tag="VF516_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8108138.1"
                     /translation="MLGPLGHVGSFVAYLATARRRHASDQTCARQLAWSGSLPPGLEL
                     TWLGTAGFRLAYQGTVVWIDPYVTRLSLRDLVARHVVPPSEAAIAAWVDRADAVLVGH
                     THFDHALDVPAIARRFGARVYGSSSLAQLMKIHGLAGQAVVVAPHRDYEVGPFRFRFT
                     PSRHSRLQLGLAVPYAGELTCEHVDELTPQRYRCGQVWGICVEVAGMRIYHQGSADLI
                     EDEIRERGVDLFLCGIAGRRFTPRYVERIVRALAPDRIVPTHYDDFFRPLDAPLRFSL
                     NVDLTGFAEEVHRASRDVPVATLEIGAPIGAASN"
     gene            complement(10560..>10752)
                     /locus_tag="VF516_10455"
     CDS             complement(10560..>10752)
                     /locus_tag="VF516_10455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108139.1"
                     /translation="RGSAWWPRVQCWSQNLAIARTIAQLSPEVARPAPRGCVQSSEID
                     RRSATEAMRDFRNALRARP"
BASE COUNT         1574 a         3673 c         3829 g         1676 t
ORIGIN      
        1 aggaacacgt ggtagacgat cttggtcgtg gcgtcgcccg gcacgcggac gcagatcggc
       61 gcgccgccgg cgtcgagcat gctcgggccg agcgcgtcgg tgaggcgcag cttgttgagc
      121 tggccggtgg tccccgcgag cgcatcgctg gttccgtagt tgtaggacag ctcgtacttc
      181 cagccgtcga gcacgcccca cgacgcgggg acgctgccgg tgacgccgag gacgatgcgg
      241 gtcgtgctga ccgtgtcgat gaactcgcgc gcgccgagct cggtcatccg ccgccggtag
      301 tcgaggagat cgccgcccag cgggttgtag aggctgtcct tgctgatcgg gctgtccgcg
      361 gtgaatgcca ccggcgacag ctggcggtca ctgctgcggt gcagatagga cacctcgagc
      421 agcagcgcgg cgtggtcgcc gagccggttg ccggcggtcc cgaacacgtt gtagcgggtc
      481 gacggcgtgt acacgtagct ctgcgtcgcc tcgttgtaga ggtcgccgga cgagaagcgg
      541 gtccagccgc cgttgccgtc gggcttgcac gcgtccgagg cacagccggg cggggccatg
      601 ccgcccgggc cgatcgacga cggatcgagc cggccgccga tcgcggcgag cgactggttg
      661 cgcgtcaccg tccggctcgc gaagtcgtag ctatcctgga actgcgagaa cgcgcgatcg
      721 ccggcgagca ccggctcgtg atgctggtag ccgcccgaga ccacgaggta cgtgttcttg
      781 tccttcgtgg tgaacccggt gacgaagctg gcgtcgtact cggtgccatc gccgtgctgc
      841 gacgtgctgg tcagcagcga cacgtcgagg ccgtcgaact gcgggcgggt gatgatgttg
      901 accacgccgc cgaccgcgtc cgcgccgtac agcgccgagg cgccgtcctt caggatctcg
      961 acgcgctcga ccatcgcgag cgggatcgtg ttgacgtcga ccgaggcgtc ggcgcccgag
     1021 ccgccgttga ccatgcgccg gccgttgacc agcacgaggg tgcgtggcgc gcccaggccg
     1081 cgcaggttga tccgggtcgt gccgtcgccg ccggcgttga cctgcgcgtt gccggcgttg
     1141 gcctgcgccg gcagcgcctg caggatgtcg ccgagcgtgg cgcggcccga cgcgtcgagg
     1201 tcggcgcggg tcacgaccga gaccggcgcc cggccggtga ccagcgtgcg ctcgatcggc
     1261 gtgtcggtca ccgagatcac ctcggtcttg ccgttgtcct cggcgggcgg ggcgccctcg
     1321 tcgccggcgg gcggcggcgc gcgcatctgc aacgagctcg gcgcgtgatc gtcggcagcg
     1381 gccggcgcgg cgaccgccac caggcacagg atcgcagcgt gcttcgtcat ccccctgctt
     1441 ggagtgacgc catcctgcat gggttgccag cgacgtacgc cgcgagacct ggcaaaatcc
     1501 ggtcgtccgc cgctcgtgtc gtcggcgcgc gcacgatgtc gtgcgacagc gcgtgcggcc
     1561 gcgctgcatc actgcacagg tgaaccctgt cgcccggcgc gacggcggat cacagcctcg
     1621 ctgtgaacgg ctggtcgaac ggcgccggca ggtcgagcgt gaacgtgagc tcgacgccgg
     1681 ccggcgcatg ggcgatcgac cacacgaggc catcgcgcga cggcgcgccg aatcgcagcc
     1741 gcaccgggct ggcgagcagg agggcccccg ccgcgggcac cgtcaccgtc gtcgggtgga
     1801 tcgggatgtc cagcgtcggc tgcacgacgg ccagcgccgc cccgtccgcc gcggcgcgca
     1861 cgcggaactg caggaacggc tcgtcgatgt ccagcgcgat cggccgcggc gtcgggttgg
     1921 tcagctcgaa gcgaatgccg tactcggcgt cgccactggg cgtcaggatc gcgcggcagg
     1981 gaggatgcgc tgtcatcgcg ggctcgcttc ggttcggggg cggggcggga ggcgtgggcc
     2041 gggggctcgg cgcaggggtg ctgggaccag gctggcagcc gagcgccagc gcgacaagac
     2101 ccagcatgcc cgcgcgcgac ggcgtcaccg cggcgccttc ttcaccttcc acgacccgtc
     2161 ggtcttgcgc acccgggtga ccatgaagcg cgcgagcgcc gtgcccggct gcgcctcgta
     2221 ggtctgcggc agcgcgacct cgatgtcctt gggggcggtc aggttgctgg cgaggttctc
     2281 gacgtcgggg tttccgccgt gccggtcgcc gacccggtcg ttggcattca cggtgaacgt
     2341 gccgttgttg ccggcgtagg ccccgatgaa gtgcgtggtg atcgccgcgg tcgatgtcag
     2401 cccgtgcgcg gtgcggtatg cctcgcggat gtcctcgggc gcgatgttgc tgatcgacca
     2461 gttgtcgaac cactccagga tcgtctggtg ctcgtagaac ggcgccgtgc cgctcggccg
     2521 cgcgggaccg atgtcgtagg tgtacctgcc gggcaggtcg acctcgccgg tgccctcgcg
     2581 ctgggtcatc gtggtcggat agtacgcctt cttgccgaag ttccaggtct cggtctgcac
     2641 caccgagccg tcggacttct tcttgagctg catcgtgata ctgaccgcat cggtgccatt
     2701 ccacggcgtc ggcacgaaga agttgacgtt gccggacgtc agtccctgga tcgtgcacgt
     2761 ggtcggcccg gtcgccgtcg tgaagtgccc gccggtggtc gtgtagacga tctcgtactc
     2821 gctggccgtt ccgccggtga actcggcgcg gcagatgatc cgctcccccg gtgcggcgag
     2881 cgggctgcgg tcgaacggca cgatgtacga cgccgtcggc gcgtcgctca cggcgcccgg
     2941 cgtgtgcggg cccggccgcg gcgtctccga gccggatggt gcgccctgcg tcgtcgcttg
     3001 ctccggcgcc ggcccctcgg tcgtcgcctg ggcgacctcg gtcgaacgcg gtccgtcggg
     3061 ctcgcgatcc gagatcgggg tcgcgctcgc gccgggccgc ccgaacaggc cgaccagtcc
     3121 ggggccgccg ctcctcggtg gcgccgcagc cgtcgaggtc gcggggacct cggtcggacc
     3181 gtcgaccagc ggctgcgacg acagccccga ggtcagcgtc cgcttgcccg ggcttgcact
     3241 gtcgtggcgc gcggccgcgc cgccgacgcc ttggtggtcc gacttggccg gttctcgaaa
     3301 catcgcttcc ccccgcaggt actgctccca tgatacgcgc acggcgagcg ccccttcctg
     3361 cctggcgagc ggtggccaca ccggcgagat ccttggcttc gatgcctggc cgaggtcggc
     3421 cacgcggcgc aggttccgcg actgtgttcc agacccatcg cagcacgcgt cgagcctgta
     3481 cccggttgcc gactgacgtg ctgcagcgtg gcgcacagcg tgagttcggc ctgcgccact
     3541 gctcccgcgc gcggagcatg tccgcggcgc ctgcgaggag cgtttcgccg cggcttctac
     3601 gtcgagttct cgggcgcggc cggcggccac cagtcggcca cgctgagtgg aaccgcatcg
     3661 aatggcgcga tccgcgcctc ggtctcgtcg ctgtacgtgc cgatcatgcg ccagtcagaa
     3721 gactccagcc ggtagacggt caacacgcgc gcctcgatgt cgacgagcca caggaagggc
     3781 acgccgacct tggcgtagta gggttgcttg cgaagcatgt cgtggcggcg cgtcgtcggc
     3841 gacaagatct cgcagaccca gtcgggcacc aggcagatcg gctcgtcggc cggcagctcg
     3901 ggcatgcgct cgcggcgcca gccggccacg tcgggcgaga tctcgggcgt gttgggcaac
     3961 tcgatcccgg gctcggtgag gatccaccag ccacctgggc ctccacgtcc atggtcaaaa
     4021 gggtcgccga ccctgccgcc gatggtgcgc gtcgtgcgct gatgccgcgc cctgggcttg
     4081 gtcatcgtgt agagcacgcc ctcgatgatc tcgccgacga tgcccggggg cagagcgtcg
     4141 aggtcagcga gcgtcggagc tctcgggctt gacttggtcg tgctcatgtc ccacctggga
     4201 ggttagcacc catggctgcg ccggccgaga tgcgactgca caggtgaacc tgtcgccccg
     4261 gcgcggctgc ggatcacagc ttcgccgtga acggctggcg aaaggcgccg tcaggtcgag
     4321 cgtgaacgtg agctcgacgc cggccggcgc gtgggcgatc gaccacacga agccgtcgcg
     4381 cgacggcgcg ccgaaccgca gccgcaccgg gctggcgagc aggaagggcc ccgccgcggg
     4441 caccgtcacc gtcgtcgggt ggatcgggat gtcgcgggtc ggctgtacga cggccagcgc
     4501 tgtcccgtcc gccgcggcgt gcacgcggaa ctgcaggaac ggctcgtcga tgtcagcgcg
     4561 atcggccgcg gcgtgggttt ggtcagctcg aagcgaatgc cgtactcggc gtcgcgcgca
     4621 catggatcgc tgatgccgtc tgcggctcgg gtcgtggtcc ctccgctggc gagccgggcg
     4681 cacgagctcg accggatcat cgtcgagtat gccggcgacg cgattgccga gctcggtgca
     4741 ccgcacgagg cattcaccgc cgacgatcgc gcgtgggtgc gggaccacgc ggcgggatcg
     4801 ctcgccgaga ttgaaaaggc cacgctgcga ctggtcacgc tctacacgtc gcgcaacctg
     4861 tctcacgcgg cggcacgcct cgggatggcg ccggtgtcgc tgagccgatg gctcggccga
     4921 cgggagttgc cccgatgacg ccctcggacc acgggagccg ggtcaacggg agccaggtcg
     4981 cggagccgct cgacgatctg cggatcgcgc tctctgaggt ggacgtcctc acgaccctca
     5041 ccaaagcggc ctacgacgac gcggactggt ccggagcgga tccggctgtc gtcgacgcca
     5101 tggctaccct gctcggcctg atcgacaagt cagcgtcctc ggccgtggct gcgttccacc
     5161 ggctgcatgg cgcgatcgcc gacgcgcagc ctgcaccggc tggtgagcgg tgggacaccg
     5221 agggccgcgg tacgaagccg ggggaaggat gacgacccgc aaaccgatca ctctctcgct
     5281 ctcggtggac tccgtcgagc agctgcaacg gatcggcgcg gcgttcttcg acgagccgaa
     5341 acccaagcag ccgctgttcc cgttcacgga cggcaactgc ttggcaaggc acgagtcctc
     5401 ggtcgtcagc cgtctgcgca agcggctcgc ggaggtgtac gggaagccgg tcgaccacag
     5461 gttcttcgag gaccagtacc tgcccgacga gcaccctaac gccgcgttgc tgcggctgtt
     5521 caggcagcgt cagatgttgc acatgaccga cgcggagacg atcgagtcgc tggcgaaggg
     5581 caggtagcgt ggcgcgcgcg ggtcgaccga cgaggacgtg atcgcggcga tgacgcgctc
     5641 gaggtaggcg catcgttcga gcgggactag gcgtccagca ttcccgacat ggccaagccg
     5701 aagcatccaa ggcagagaaa gcccgctccg gcgatcacca gcaggatggg gccgatgagt
     5761 tgtttccacg ggaccgtcgt aaagagatgc gccgttaccg cccgcgccga cggcacgcgt
     5821 ggtgtgtcat cgtcaccgtt ggggacgggg ttgcgcttct ccagccagcg gccagcgatg
     5881 acgagggcaa taagccccgc cgtcaagccc atgaacacga gccgcccacc ccagggtccc
     5941 catcccagcc atctcagcca tcccagccgc tcctgccacg ccggatcgat gccaaccacc
     6001 atccccacga tcagaccaaa cggcgccatg ccgcacagcg ccccgaagca caccagcagt
     6061 accaccggtg cgatcagcag ccccgccacg gggtgattcg cgatagccgc ctcgatggca
     6121 gccatgagtc cgatgatgag cgagcagccg acgtatccgg ccaggaacga cctcatccca
     6181 ccggccagga gccacctccg caagatcggc cgcatgggcg cgagtatatc tcagcgccgg
     6241 gcggcggaag tgacgcgacc gtgacgcggc gctggcggtg ctcgccgaga tcgagggcgc
     6301 gatcgacttc cccgaagtag gcccgcgcca gctcctcgat cggcggcggg aacgggtcgg
     6361 atcgacgtcg acgcgtgacg gctcaccccc tccacccgct cgccgcggca cgctggctga
     6421 cgtcggccaa gctcgacgcg gccgaggctg ttcgaggcgg tgctgccgtg ctgaccgtgg
     6481 gcgaggacat cgcggtcgcc cagcgcgcgc aggcgatcgc cgcgaccgac caccgcgcgc
     6541 tgatccaggc gcagcgtgag ctcgccgctg ccggggccga gatcaagctg ctgctgcggc
     6601 ggcggccctc aacgtgagcg cacacgcatc gctgccgcgc cggtggccga aaccagttct
     6661 gtgcccggac gtagtgtgta ccgcagcatc aattcttgcc ctgcatttgc aggatcgcgg
     6721 ctcgcagtgc ggcgagccaa ggatcgaagt cgcgccgttc tgccatggcg gcgatccgga
     6781 tgctgtggcc atcatcgaaa tcgatccgga tatacggcgt gacgctatgg attcgaccgc
     6841 cctgctgaac gacgccgcga ccgagccagt gcggcgcgcc catgtggacg ccctgatagg
     6901 ccgggagctc tggaagaact cgcttgatga gcgcctgcgt gttggtgccc aactcgcgga
     6961 ttgcaatacc gccgcgatac gcgcgcaatg cacgtagttc gccgcgtacc gagtcgggaa
     7021 acgcgcgcga ggggtcgagg atgaacgatc cctgcgggcc gatctcgaca ccggcacgat
     7081 cgagctcagc cacctgttgc agctcgtgag cgaacgaggc gatgcactgc tggacctcag
     7141 gggcgaagca atcgacccat gtacccagga gcagccaggc ctctccagca gcgttcggag
     7201 gcatgtaatc gatgccgccg aagtcgccgc cgcggtaacg tgcgatcgcg gcgcgcagca
     7261 cggcctcgct gcgtgcaccg aagccgtgca ggacgaacac gccagcgagg acgtcctgga
     7321 tcaccactgc atcttgccac agggccagcg gccggggcat tccccgtgca gctgtcgctc
     7381 aatcgaggcc ggtgaccgcg cgagccccgc cgaaacctca cgatagccaa gcgatgtgac
     7441 cggcctgatg aaagcggccc cgctgctggc gcagcgcaag cactgagaac cacacgatca
     7501 tggccctcga ggtctccatg cgctccgccg ctgcagcgat cgtggccttc gatgtcttcg
     7561 tccacaatcc cgatcgtcgc cgtaagaacc cgaacctctt cctgtgcgac cgagatctcg
     7621 tcgtgttcga ccagccgccg aacacgtctt cgtggacccg ctgctcaacc cccacgtgga
     7681 tccccggaag ctcctcgcgc tcaagagctc gatgacgctg cacgactggg agacgcagat
     7741 ccgcgggaag atgctgcagc ttcccgatcg cgtgctgtac acgtgggatc gcgcggtgaa
     7801 cgagcgctcg gtgccggacc tcgggcggat cacgcgcgag ttcctgaccg cctgggaggg
     7861 cgaccggacg cgctgggacc agctgatcgc ggtcgacgtg cagcgcttcc cgtgggtcgc
     7921 ggtcggcatc ttcgacgtgt accgcgaccc gcgcacgccg ggcgatgcga agacgcggct
     7981 gctctggatg gtcgacgaga tcgcgctgtc cgccggcgat gaggtcgacg tgtgccgcga
     8041 gctcgcggcc aagggctacg acggcgagcg gtgcctggtc gtcacggatg cgtcgtgcga
     8101 gtggcagcag ctcgagcgcg acctgatccg acagcgcccg aactaccagg gcaagggcac
     8161 caactacatc ctgcgcgaga acgggttccc gcacgtcgtg ccgccggacc gcagcgcgaa
     8221 ggccaacccc ggcatcctcg agcggatccg ggcgacgaac gcgtgcatcc gcgagggccg
     8281 cgcgatcctc ggcggcaagg cgacgatcga ggtgctgaac caggcgctgc gcagcggcag
     8341 cggcgcgggc gaccacctcc accccgggat cgtctcgctc tcgaacagcg agatctcgaa
     8401 ggccatcacc gccggcacgc tgaccagcga caccggcggc cctggatcgt tcgcgctcgg
     8461 ccaggtgcac gccgatcgcg cgcacaagct ctcgctcgcc gacgcgcgcc ggatcggcaa
     8521 tgtgttccgt cgcgacctcg ggcgcgagta ccaggcgatc tccgagctcg ccgagcgcga
     8581 ccgtcggccg ctgacgatgt gggtccgcca gcggcttgag gacgccgtgg ccgccgcccg
     8641 cgctgcgagc acaaccggag ccggtacccg gagcggtgag cccccggaag cttcgctcgc
     8701 tcaccgcggt cgctggcagg ctcatcgcgg cgttccgtgc cgttcgagcg atacctcgcg
     8761 atacgctgga tcccgcggca gcgcggcgac gactgcgcgc gaggcggcgg agatcgagga
     8821 gccgttcggc tacggccgag ccggacgccc aagccgccgt ggagcctcgc gcctccgggg
     8881 gagacgtcgg ggagacacgg cgatgagctt gctcaattgc tggtgattca gatggcttac
     8941 ggagaggcta cgcgatgttc ttgttcgcga agtcccagtt gacgatgttc cagaacgcct
     9001 cgatgaactt ggggcgcgcg ttgcggtagt cgaggtagta ggcgtgctcc cagacgtcga
     9061 gggtcagcac gcacttcttg ccgaccgcga acggggtgtc ggcgttggac gtggtctcga
     9121 tcgcgagcga gccgtcggcg ttcttgacca gccacgccca gcccgagccg aactggccgg
     9181 ccgcggcctc gctgaacttg gtcttgaagt cggcgaagtt gccgaacgac ttgtcgatcg
     9241 ccgcggcgat cgcgccggtc gggttgccgc cggccttggg ggcgagcgag tgccagaaga
     9301 acgtgtggtt ccagacctgg gccgcgttgt tgaagatctt cttctcgctg gcctggccct
     9361 gcgatgcctt gatcacgtcc tcgagcgaca tcgactcgta cttggtgctc ggggcgaact
     9421 tgttcaggtt gtcgacatag gccttgtggt gcttgccgta gtggtactcg atcgtctcgg
     9481 cagagatcga aggcgcgagg gcctccttgg cccagggcaa ctcggggagg gtgaacgcca
     9541 tggtggtctc cttttggttg ggactggttg tagcgcttgg gtcccgccca ggggagcctc
     9601 tggcgctgta aagtgatggc gtgctcggtc cgctcggcca cgtcgggagc ttcgtcgcgt
     9661 acctcgcgac cgcgcgccgc cggcatgcca gcgatcagac gtgtgcgcgg cagctcgcgt
     9721 ggtcgggcag cctgccgccc gggctggagc tgacctggct cggcacggcg gggtttcgcc
     9781 tcgcgtacca gggcacggtc gtgtggatcg acccgtatgt cacgcggctc tcactgcgcg
     9841 acctcgtcgc gcgccacgtg gtgccgccgt cggaggccgc gatcgcggcg tgggtcgatc
     9901 gcgccgacgc ggtgctggtc ggccacaccc acttcgatca cgcgctcgac gtgccggcga
     9961 tcgcgcggcg gttcggcgcc cgggtctacg gctcgagctc gctggcgcag ctgatgaaga
    10021 tccacggcct cgccggccag gcggtggtgg tcgcgccgca ccgcgactac gaggtcgggc
    10081 cgttccggtt tcgcttcacg ccgagccggc actccaggct ccagctcggg ctggccgtgc
    10141 cctacgccgg cgagctgacc tgcgagcacg tcgacgagct gacgccgcag cgctaccgct
    10201 gcggccaggt ctgggggatc tgcgtcgagg tcgccgggat gcggatctac caccagggct
    10261 cggccgacct gatcgaggac gagatccgcg agcgcggggt cgacctgttc ctgtgcggca
    10321 tcgccggccg ccggttcacc ccgcgctacg tcgagcggat cgtgcgtgcg ctggcgcccg
    10381 accggatcgt gccgacccac tacgacgact tcttccgccc gctcgacgcg ccgctgcggt
    10441 tctcgctcaa cgtcgacctc accgggttcg ccgaggaggt ccaccgcgcg tcgcgcgacg
    10501 tgccggtggc gacgctggag atcggcgcac cgatcggcgc cgccagcaac tgacgcgggc
    10561 tacggccggg cgcgcagcgc gttgcggaag tcgcgcatcg cctcggtggc gctgcggcgg
    10621 tcgatctcgg aggattgtac gcatcctcgg ggtgcaggac gcgcgacctc tggcgagagc
    10681 tgtgcgatgg tccgcgcgat ggccaagttc tggctccagc actgcaccct tggccaccat
    10741 gcggatccgc gt
//
LOCUS       DASVOR010000520         3243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443033,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000520 DASVOR010000000
VERSION     DASVOR010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443033"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..886
                     /locus_tag="VF516_10460"
     CDS             146..886
                     /locus_tag="VF516_10460"
                     /inference="COORDINATES: protein motif:HMM:NF003814.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamB/YcsF family protein"
                     /protein_id="HEX8108140.1"
                     /translation="MTTINCDMGESFGLYKMGDDNALMPLISVANVACGFHASDFNHM
                     RATVRLAKQHGVQVGAHPSLPDLQGFGRREMKIGREELANCLIYQIGALAGFLKAEGM
                     PLGHIKPHGSLYGMAGRMEDIAHAICDAADVFGVPIFGMIGTLHETVYLARGHAFVAE
                     YYADLDYSDDGKLIITREHPAVDPAQAAARCLRAVRDGVTRSTGGVDIRVRADSICVH
                     SDTPNAAAVAAAVRAALTTSLSSPGEGA"
     gene            888..1130
                     /locus_tag="VF516_10465"
     CDS             888..1130
                     /locus_tag="VF516_10465"
                     /inference="COORDINATES: protein motif:HMM:NF005457.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEX8108141.1"
                     /translation="MAVKQILSPVPGTFFRRPSPDQPPYKEVGDPVAAGDVVGLVEVM
                     KTFYPVTSDAAGRVRGFLVENEGEVQAGQALVALEE"
     gene            1134..2027
                     /locus_tag="VF516_10470"
     CDS             1134..2027
                     /locus_tag="VF516_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014712.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyltransferase domain-containing protein"
                     /protein_id="HEX8108142.1"
                     /translation="MALHAARPARYSFGGDEHVFVEVDEEMSLEAFFKSLALTRAVRE
                     AQITGVTEICPANASFQIKFDPGRIHPDDMLAELRRIEAAAARAESRLHTRIIEIPVF
                     YRDPWTTETLMRFRERHQDPRSTDIEYAARINGYASVDDFIRAFSDSPWFVSMVGFVA
                     GLPFLYQMVERKRQIQVPKYLRPRTDTPKLTVGFGGCFGCIYSVRGAGGYQMFGITPM
                     PIYDPAQQISYLRDFMVLFRPGDIVQWQPIDRTAYDAITADVEAGRFAPRIREVTFDL
                     DEFNADVDGYNARLKGALHGN"
     gene            2017..2991
                     /locus_tag="VF516_10475"
     CDS             2017..2991
                     /locus_tag="VF516_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEX8108143.1"
                     /translation="MAIRVLAPGLATTVQDLGRPGYYHLGIPLSGAMDRFALRAANLL
                     VGNDEGAAGLECVFLGPQLEFTASATVAVTGAELIPKVDGAPRATWTSFAVAAGQVLS
                     FEYLKAGARAYLAVSGGIDVPVVLGSRSTYPLGALGGYQGRGLQAGDELAAHPSSGTP
                     EGRTVPPELRPATGKPAVLRVVPGLYWHRITEQAGRDFFADPWKVAPEADRIGYRFRG
                     GRPLQFVPREQPFGAGSDPSNIVDACYPYGSIQVPGGTEPIVLHRDAVSGGGYFMIGT
                     VISADMDLVGQLQPHTEVRFVEVDMAGALNARRDREARLARVRNAISG"
     gene            complement(3057..>3243)
                     /locus_tag="VF516_10480"
     CDS             complement(3057..>3243)
                     /locus_tag="VF516_10480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108144.1"
                     /translation="DTLRAELHRRGRLDDVEARLADLDRAMQAFTAVHRARQDREVLA
                     PVIEAVREAARRGRSSP"
BASE COUNT          482 a         1162 c         1086 g          513 t
ORIGIN      
        1 tcggcggcac gttctacaac ggcgcgaaga cggcgatcga cgcggttccg atcctcggcc
       61 cggcgatcac gaaccggctg tagcggccgg gccgcgcgca ccttgcccga ccctgattcg
      121 tggcaggata gcgccggagc gcggcatgac gacgatcaac tgcgacatgg gcgagtcctt
      181 cggcctgtac aagatgggcg acgacaacgc gctcatgccg ctgatctcgg ttgccaatgt
      241 cgcctgcggt tttcacgcgt ccgacttcaa ccacatgcgg gcgaccgtcc ggctcgccaa
      301 gcaacatggc gtccaggtcg gcgcgcatcc ctcgctgcct gatctgcagg ggttcggccg
      361 gcgcgagatg aagatcggcc gcgaggagct cgccaactgt ctgatctatc agatcggcgc
      421 gctcgccggc ttcctgaagg ccgaaggcat gccgctcggc cacatcaagc cgcacggctc
      481 gctctacggc atggccggcc ggatggagga catcgcgcat gcgatctgcg acgcggccga
      541 cgtgttcggg gtgccgatct tcggcatgat cggcacgctg cacgagaccg tctacctggc
      601 gcgcggccat gccttcgtcg ccgagtacta cgccgacctc gactacagcg atgacggcaa
      661 gctgatcatc acgcgcgagc atcctgcggt cgatccggcg caggcggctg ctcgctgtct
      721 ccgtgccgtc cgcgacggcg tcacccgcag caccggcggc gtcgacatcc gggtgcgcgc
      781 cgattcgatc tgcgtccatt ccgacacgcc gaacgcagcc gccgtggccg ccgcggtccg
      841 cgcggcgctc acgacgtctc tgtcatcgcc cggggaagga gcctgacatg gccgtgaaac
      901 agatcctgtc gcccgtcccg ggcacgttct tccgccgtcc gtcgccggac cagccgccct
      961 acaaggaggt gggcgacccg gttgcggcgg gcgacgtggt cggcctcgtc gaggtgatga
     1021 agaccttcta cccggtgacc tccgatgccg ccgggcgagt ccgcggcttc ctggtcgaaa
     1081 acgagggcga ggtccaggcc gggcaggcgc tggtcgccct ggaggagtga gccatggcgc
     1141 tgcacgcagc acgaccagcc cgctattcgt tcggcggcga cgagcacgtc ttcgtcgagg
     1201 tcgacgagga gatgtcgctg gaggcgttct tcaagagcct ggcgctgacc cgcgccgttc
     1261 gcgaggccca gatcaccggc gtcaccgaga tctgcccggc gaacgcgtcg ttccagatca
     1321 agttcgatcc cggccggatc catcccgacg acatgctggc cgagctacgg cgcatcgagg
     1381 cggccgcggc cagggcggag tcgcgcctcc acacacggat catcgagatc ccggtgttct
     1441 atcgcgatcc gtggaccacc gagacgctga tgcggtttcg cgagcgccat caggacccca
     1501 ggtcgaccga tatcgagtat gccgcgcgca tcaacggcta cgcctcggtc gatgacttca
     1561 tccgggcgtt ctcggactcg ccgtggttcg tgtcgatggt cggcttcgtc gccggactgc
     1621 cattcctcta ccagatggtc gagcgcaagc gccagatcca ggtgccgaag tacctgaggc
     1681 cacgcaccga cacgccgaag ctgaccgtcg gcttcggcgg ttgcttcggc tgcatctact
     1741 cggtgcgcgg cgccggcggt taccagatgt tcggcatcac gccgatgccg atctacgacc
     1801 ccgcccagca gatcagctac ctccgggact tcatggtgct gtttcgcccc ggtgacatcg
     1861 tccagtggca gccgatcgat cggaccgcgt acgacgcgat caccgccgac gtcgaggccg
     1921 ggcgcttcgc gccgcggatt cgcgaggtca ccttcgatct cgacgagttc aacgccgacg
     1981 tcgacggcta caacgccagg ctgaagggag cgctccatgg caattagggt cctcgctccc
     2041 gggctcgcca ccaccgtgca ggacctgggt cgtcccggct attatcatct cggcatcccg
     2101 ctgtccggcg ccatggaccg gttcgcgctg cgcgccgcca acctgctggt cggcaacgac
     2161 gagggcgcgg cgggtctgga atgcgtgttc ctcggcccgc agctcgaatt cacggcgagc
     2221 gccacggtgg cagtcaccgg agcggagctg atcccgaagg tcgacggggc gccgcgcgcg
     2281 acctggacgt ccttcgcggt cgccgcgggg caggtgctct cgttcgagta tctcaaggcg
     2341 ggtgcccgcg cctacctcgc ggtatcgggc ggcatcgacg tgccggtcgt gctcggcagc
     2401 cgctcgacgt atccgctcgg tgcgctcggc ggctaccagg gccgcgggct gcaggccggt
     2461 gacgagctcg ccgcacatcc ctcatccgga acgccagagg gccgcaccgt cccacccgag
     2521 ctccgtcctg cgaccggcaa gccggccgtg ctgcgcgtcg ttccgggcct gtactggcac
     2581 cgcatcacgg aacaggcagg ccgcgacttc ttcgccgatc cctggaaggt cgcgcccgag
     2641 gccgaccgga tcggctaccg cttccgcggc ggccggccgc tgcagttcgt gccgcgcgag
     2701 cagccgttcg gcgccggctc cgatccgtcg aacatcgtcg acgcctgcta tccctacggc
     2761 tcgatccagg tccccggcgg caccgagccg atcgtcctcc accgcgacgc ggtctccggt
     2821 ggcggctact tcatgatcgg cacggtcatc tccgcggata tggacctggt cggccagctg
     2881 cagccacaca ccgaggtacg cttcgtcgag gtcgacatgg ccggcgcact gaacgctcgc
     2941 cgcgaccgcg aggcgcgcct ggccagggta cggaacgcga tctcgggctg accgctcgac
     3001 ctcccagcgg tgacgcggcc ggcacggcca tgtcgcatgc ggcgagccaa cgcttgctac
     3061 ggcgacgacc gcccgcgccg cgcggcctcg cgcaccgcct cgatcaccgg ggcgagcacc
     3121 tcgcgatcct ggcgcgcgcg gtggaccgcg gtgaacgcct gcatcgcgcg gtcgagatcg
     3181 gcgagccggg cctcgacgtc gtcgagccgg ccccggcggt gcagctcggc gcgcagcgtg
     3241 tcg
//
LOCUS       DASVOR010000521         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443035,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000521 DASVOR010000000
VERSION     DASVOR010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443035"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            183..2909
                     /locus_tag="VF516_10485"
     CDS             183..2909
                     /locus_tag="VF516_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108145.1"
                     /translation="MPSQLASATLIAPRKRPLGFELALVVLVALAVLAPGIWGYSLVD
                     PWETHYGEVARVMLQNHDWVHTDWPQESEGFRSKPVLMFWMMAASLRAHGLAAGGGYS
                     GEMVHDATTMIAIRLPFVLCAAFGLVLMWWMLARLVSRRLAWLALLVVGSCPFMCLIA
                     RQAMPDMPLTACVIGALSLFAMALEDGERPVAPFRSFRLGGRTIALDARHVVIGLALA
                     VLIVQAIYYAVYFAMSPALMVRGRMPSPALWLPLVMAVAGFALHRDGWLFARLPFLLI
                     GGIVAAIVNAPVRARAPGQRLWRHVFDDVLAVWDRYAADRYLARVATYLAIPLAALLG
                     SLAVLSVIDPATAPIRSLEIAGGVTVIWAWIGIAAPGHWPLPNWRDTGPIVDRMLAMA
                     PITTMRQVYLIGCYSLLGISVLAKGPPGVAVVGAVGVFQVVMYHRWRALYGGAYEIKR
                     GLILMIATFLPWHVAMYLKDGLQFINDYVFQHVLNRAAVGVDNSPGTFEYYASQIGHG
                     MWLWAALLPAALTAAILRTRTDTREGRVRFLVMLWAVAGFAFFGLVQTKFHHYILPVV
                     PALGLLVAFFLDDLAAGRDRLHPVFAAIGVAIVLLVCRDLMHEPERWIEMFVFRYDRP
                     WPAGEPYAVDPSDGVLALGIASAISVAVAATRWRRLGAVCVGAAGLAICVWALQVYMP
                     IAGTHWGMRDAVRAYYDQRTIYGEKRVYFGLGELYDDWRDAGDRWAFETHVPDNLQIG
                     QPMTLQLEVHKADDDRVTEHELSLVGTVTAIADHRVELALAPGERARLDPLIARGKAA
                     PRGRPAVHVVDADRLIAWQLYWRGENFWSGDEIWGYPPEMKTAFKEANNVAFTKYIND
                     RARAPIGRRYFLITEAGRITAPRASLPTQRARDSYEVLDTTSNKFSLAAFWL"
     gene            complement(2917..>3154)
                     /locus_tag="VF516_10490"
     CDS             complement(2917..>3154)
                     /locus_tag="VF516_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEX8108146.1"
                     /translation="LTEASFTLTDRSMGINYGLNKVRFTSPVPAGSKIRARFTLTKLD
                     KLDGGVQTTWSVVVEREGEGKPVMVAETLGRHYR"
BASE COUNT          388 a         1147 c         1107 g          512 t
ORIGIN      
        1 cgcacgcggc gcgccggatc gcaggcggcg atcacgtcgc gcagcaccgc ctcggcgagc
       61 ccgcggtgaa gcccggcgcg cgtcatcgga tcgccatcgg cgggtgaccg gtcggcatcg
      121 ccatcctccg gcgcccgagc atagcagcgg gccgcgcgcg ccgcgtgcta ggttcgcacg
      181 cgatgccgtc ccagctggcg agcgccacgc tgatcgcgcc ccgcaagcgc ccgctgggct
      241 tcgagctcgc gctggtcgtc ctggtcgcgc tcgccgtgct ggcgccgggg atctggggct
      301 actcgctggt cgacccgtgg gagacccact acggcgaggt cgcccgggtg atgctgcaga
      361 accacgactg ggtccacacc gactggcccc aggagagcga gggctttcgc agcaagccgg
      421 tcctgatgtt ctggatgatg gccgcctcgc tgcgcgccca cgggctcgcg gccggcggcg
      481 gctactccgg cgagatggtg cacgacgcga ccacgatgat cgcgatccgg ctgccgttcg
      541 tgctgtgcgc ggcctttggc ctggtcctga tgtggtggat gctggcgcgc ctggtcagcc
      601 ggcggctggc gtggctcgcg ctgctcgtcg tcggctcgtg cccgttcatg tgcctgatcg
      661 cgcgccaggc gatgcccgac atgccgctga ccgcgtgcgt gatcggcgcg ctgtcgctgt
      721 tcgcgatggc gctcgaggac ggcgagcggc cggtcgcgcc gttccggtca ttccgcctcg
      781 gcggccggac gatcgcgctc gacgcgcgcc acgtcgtgat cggcctcgcg ctcgccgtgc
      841 tcatcgtcca ggcgatctac tacgcggtct acttcgcgat gtcgccggcg ctcatggtgc
      901 gcggccggat gccgagcccg gcgctgtggc tgccgctggt catggccgtc gccgggttcg
      961 cgctgcaccg cgacggctgg ctgttcgccc ggctcccgtt cctgctgatc ggcggcatcg
     1021 tcgcggcgat cgtcaacgcg ccggtccgcg cgcgcgcgcc ggggcagcgc ctgtggcggc
     1081 acgtgttcga cgacgtgctc gcggtgtggg accgctacgc ggcggaccgc tacctcgccc
     1141 gggtcgcgac ctacctggcg atcccgctcg cggcgctgct cggatcgctc gcggtgctca
     1201 gcgtcatcga ccccgccacc gcgccgatcc ggtcgctcga gatcgccggc ggcgtgaccg
     1261 tgatctgggc ctggatcggc atcgccgcgc ccggccactg gccgctgccg aactggcgcg
     1321 acaccgggcc gatcgtcgat cgcatgctcg cgatggcgcc gatcaccacg atgcgccagg
     1381 tctacctgat cggctgctac tcgctgctcg ggatcagcgt gctggccaag ggcccgcccg
     1441 gcgtcgcggt ggtcggtgcg gtcggcgtgt tccaggtcgt gatgtaccac cgctggcgcg
     1501 cgctgtacgg cggcgcctac gagatcaagc gcggcctgat cctgatgatc gcgacgtttt
     1561 tgccctggca cgtcgcgatg tacctcaagg acggcctgca gttcatcaac gactacgtgt
     1621 tccagcacgt gctcaaccgc gccgcggtcg gcgtcgacaa ctcgcccggc accttcgagt
     1681 actacgcgtc gcagatcggc cacggcatgt ggctgtgggc cgcgctcttg ccggccgcgc
     1741 tgaccgccgc gatcctgcgg acgcgcaccg acacccgcga gggccgggtc cggttcctgg
     1801 tcatgctgtg ggcggtcgcc gggttcgcgt tcttcgggct ggtccagacc aagttccacc
     1861 actacatcct cccggtggtg ccggcgctcg gcctgctcgt cgcgttcttc ctcgatgatc
     1921 tggcggccgg ccgcgaccgg ctgcacccgg tgttcgcggc gatcggcgtc gcgatcgtgc
     1981 tgctggtctg ccgcgacctg atgcacgagc ccgagcgctg gatcgagatg ttcgtgttcc
     2041 gctacgaccg gccgtggccc gccggcgagc cgtacgcggt cgacccgtcc gacggcgtgc
     2101 tcgcgctcgg catcgcctcg gcgatatcgg tcgcagtcgc ggcgacccgg tggcgccggc
     2161 tcggcgcggt gtgcgtcggc gcggccgggc tggcgatctg cgtgtgggcg ctccaggtct
     2221 acatgccgat cgcaggcacc cactggggca tgcgcgacgc ggtgcgcgcg tactacgacc
     2281 agcgcacgat ctacggcgag aagcgggtgt acttcggcct cggtgagctg tacgacgact
     2341 ggcgcgacgc cggcgaccgc tgggcgttcg agacccacgt ccccgacaac ctccagatcg
     2401 gccagccgat gacgctccag ctcgaggtcc acaaggccga cgacgatcgc gtcaccgagc
     2461 acgagctgtc gctggtcggc acggtgaccg cgatcgccga ccaccgcgtc gagctcgcgc
     2521 tggcgcccgg cgagcgcgcc cggctcgatc cgctgatcgc gcgcggcaag gcggcgccgc
     2581 gcggccggcc cgcggtgcac gtggtcgacg ccgaccggct gatcgcatgg cagctgtact
     2641 ggcgcggcga gaacttctgg tccggcgacg agatctgggg ttacccgccg gagatgaaga
     2701 ccgcgttcaa ggaagccaac aacgtcgcgt tcaccaagta catcaatgac cgcgcccgcg
     2761 cgccgatcgg ccgccgctac ttcctgatca ccgaggccgg tcggatcacc gcgccgcgcg
     2821 ccagcctgcc gacccagcgc gcccgcgaca gctacgaggt gctcgacacc acgagcaaca
     2881 agttctcgct cgccgcgttc tggctgtgac gcgcggctaa cggtagtgcc ggccgagggt
     2941 ctcggcgacc atgaccggct tgccctcgcc ctcgcgctcg accaccacgc tccaggtggt
     3001 ctggacaccg ccgtccagct tgtccagctt ggtcagcgtg aaccgggcgc ggatcttcga
     3061 tccggccggg accggcgacg tgaaccgcac cttattcagc ccgtagttga tccccatcga
     3121 ccggtcggtc agcgtgaacg aggcctcggt cagc
//
LOCUS       DASVOR010000522         3053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_180314,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000522 DASVOR010000000
VERSION     DASVOR010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180314"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VF516_10495"
     CDS             <1..1046
                     /locus_tag="VF516_10495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEX8108147.1"
                     /translation="SATAPAAFPPGPSATPTAPYPAVTASGAIQVVDATATHQRVLAT
                     PATRRLARQLGVEIGRVPPTGPHGRVTTADVQRYQSNGGAAAHAESPAAPAPQHAPAP
                     PIASISAPGRDGDEERIPLRGIRRRIAESMARSKRTAAHFTYVEEIDMTELVAVRERA
                     KARAAERGVKLNYLPFIVKACVSGLKKWPQLNASLDETTQEIVRKKYYHIGIAAQGPQ
                     GLSVSVVRDADRRSIFDLSREIDRLAEAVRGSTATREELTGSTFTITSLGKLGGVLAT
                     PIINFPEVAILGVHKIEERPAVRHGQIVIRHLMNLSISVDHRLADGWDGAMFVQDVKS
                     LLEDPTTMFMEMV"
     gene            1047..2180
                     /locus_tag="VF516_10500"
     CDS             1047..2180
                     /locus_tag="VF516_10500"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8108148.1"
                     /translation="MEYFDGDDDARARSLFQLVREDGTPVDPGALADLDRALARRLFE
                     GMVRIRVTDARMMALQRQGRIGFYGEAVGQEAAIVGSAAASEPQDWIVPALREAGVGL
                     YRGMTLDSYIAQIFGNAADPSKGRQLPCHPCDRAHHYVVMSSCVSTQIPHAVGIAMAM
                     KIAGDRGKVCFGFMGDGGTSEPDFHVALNFAGVSGAPCVLICQNNQWAISTPGRVQTA
                     AETLALKGVGYGIEALRADGNDVLAVYHAVKHAADRARRGDGATFVELLTYRVSAHSS
                     SDDPSRYRDESVTEVWRGSRDPIRRMETYLIARGWLAAGEREAIAQRLEAEVRDAIAR
                     QEAIPPPALETLFDDVYEHPTPRLREQLAELAAAPRVRAPHQH"
     gene            2228..>3053
                     /locus_tag="VF516_10505"
     CDS             2228..>3053
                     /locus_tag="VF516_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8108149.1"
                     /translation="MSEQRFDAVVIGAGPGGYPAAIRLGQLKVKTAIIEREYIGGVCL
                     NVGCIPSKAVIHAAKMFDKLGHSEDIGISIPGKPVLDMARLQAWKGGVVGKLTGGVRT
                     LLKANKVEILEGNATLGKPGPDGHRITVKSASGEQTILAKSVVIATGSRPIEIPGFKI
                     DQGRIIDSTGALALTAVPPRLIVIGGGYIGLELGMCYAKFGSKVTVVEALPRLLGTMD
                     KDCVAVVDRKLKKMGVEVMVDTKAKAWEDKGDRAVLTVELKDGKSAAIDADKILLSV"
BASE COUNT          473 a         1109 c         1068 g          403 t
ORIGIN      
        1 ccagcgccac cgcaccagcg gcgttcccac cgggcccctc ggcgaccccg accgcaccgt
       61 atccggctgt gactgccagc ggcgcgatcc aggtcgtcga cgccaccgcg acgcaccagc
      121 gcgtgctcgc gaccccggcg acccggcggc tggcgcgcca gctcggcgtc gagatcggcc
      181 gcgtgccacc gaccgggccg cacggccggg tcaccaccgc cgacgtccag cgctaccagt
      241 ccaacggcgg cgcggctgcc cacgccgaga gccccgcggc cccggcgccg cagcacgccc
      301 cggctccccc gatcgcatcg atctccgccc ccggacggga cggcgacgag gagcggatcc
      361 cgctgcgcgg catccggcgg cggatcgccg agtcgatggc gcggtccaag cgcaccgcgg
      421 cgcacttcac gtacgtcgag gagatcgaca tgaccgagct ggtcgccgtg cgcgagcgcg
      481 ccaaggcgcg ggccgccgag cgcggggtca agctcaacta cctgccgttc atcgtcaagg
      541 cgtgcgtgtc cgggctcaag aagtggccgc agctcaacgc gagcctcgac gagacgaccc
      601 aggagatcgt gcgcaagaag tactaccaca tcgggatcgc cgcccagggc ccgcagggcc
      661 tgtcggtcag cgtggtgcgc gacgccgatc ggcggtcgat cttcgacctg tcgcgcgaga
      721 tcgatcgcct ggccgaggcg gtgcgcggca gcaccgcgac ccgcgaggag ctcaccggat
      781 cgacgttcac gatcacctcg ctcggcaagc tcggcggcgt gctcgccacg ccgatcatca
      841 acttccccga ggtcgcgatc ctcggcgtgc acaagatcga ggagcggccg gcggtgcgcc
      901 acggccagat cgtgatccgc cacctgatga acctgtcgat ctcggtcgac caccggctcg
      961 ccgacggctg ggacggcgcg atgttcgtcc aggacgtcaa gtcgctgctc gaggacccga
     1021 cgacgatgtt catggagatg gtctaggtgg agtacttcga cggcgacgac gacgcccggg
     1081 cgcgcagcct gttccagctc gtccgcgagg acggcacgcc ggtcgacccc ggtgcgctcg
     1141 ccgacctcga ccgcgcgctc gcgcgccggc tgttcgaggg catggtccgg atccgggtga
     1201 ccgacgcccg gatgatggcg ctgcagcgcc agggccggat cgggttctac ggcgaggcgg
     1261 tcggccagga ggccgcgatc gtcggctcgg ccgcggcgag cgagccccag gactggatcg
     1321 tcccggcgct gcgcgaggcc ggggtcgggc tgtaccgcgg catgacgctg gactcgtaca
     1381 tcgcccagat cttcggcaac gccgccgatc cgtcgaaggg ccgccagctg ccgtgccatc
     1441 cctgcgatcg cgcgcaccac tacgtcgtga tgtcgtcgtg cgtgtcgacc cagatcccgc
     1501 acgcggtcgg catcgcgatg gcgatgaaga tcgccggcga tcgcggcaag gtgtgcttcg
     1561 gcttcatggg cgacggcggc acgagcgagc ccgacttcca cgtcgcgctc aacttcgcgg
     1621 gggtgtcggg cgcgccgtgc gtgctgatct gccagaacaa ccagtgggcg atctcgacgc
     1681 ccggtcgcgt ccagaccgcg gcggagaccc tcgcgctcaa gggcgtgggc tatggcatcg
     1741 aggcgctgcg cgccgacggc aacgacgtgc tcgcggtgta ccacgcggtc aagcacgcgg
     1801 ccgaccgggc gcgccgcggc gacggcgcca cgttcgtcga gctcttgacc taccgggtca
     1861 gcgcgcattc gtcgtcggac gatccgtcgc gctaccgcga cgagtcggtc accgaggtgt
     1921 ggcgcggctc gcgcgatccg atccggcgaa tggagaccta cctgatcgcg cgcggctggc
     1981 tcgccgccgg cgagcgcgag gcgatcgcac agcgcctgga ggccgaggtc cgcgacgcga
     2041 tcgcgcgcca ggaggcgatc cccccgcccg cgctcgagac cctgttcgac gacgtctacg
     2101 agcacccgac cccgcggttg cgcgagcagc tcgccgagct cgccgccgcc ccgcgcgtcc
     2161 gcgcgccgca ccagcactga ccggtccgcc ggggcccgat cgcgggcgcg cgtggtatga
     2221 cgcggccatg tccgagcaac gtttcgacgc cgtcgtgatc ggagccggcc ccggcgggta
     2281 tcccgccgcc atccggctcg gccagctcaa ggtcaagacc gccatcatcg agcgcgagta
     2341 catcggcggc gtgtgcctca acgtcggctg catcccgtcg aaggccgtga tccacgccgc
     2401 caagatgttc gacaagctcg gccactccga ggacatcggc atctcgatcc cgggcaagcc
     2461 ggtgctcgac atggcccggc tccaggcctg gaagggcggc gtggtcggca agctcaccgg
     2521 cggcgtccgc accctgctca aggccaacaa ggtcgagatc ctcgagggca acgcgaccct
     2581 gggcaagccc ggccccgacg gtcaccggat caccgtcaag tccgcatccg gcgagcagac
     2641 gatcctcgcc aagagcgtcg tgatcgcgac cggctcgcgg ccgatcgaga tccccggatt
     2701 caagatcgac caggggcgga tcatcgactc gaccggcgcg ctcgcgctca ccgcggtgcc
     2761 gccgcggctg atcgtgatcg gcgggggcta catcggcctc gagctcggca tgtgctacgc
     2821 caagttcggc agcaaggtca ccgtggtcga ggcgctgcct cggctactcg gcacgatgga
     2881 caaggactgc gtcgccgtgg tcgaccgcaa gctcaagaag atgggcgtcg aggtcatggt
     2941 cgataccaag gcgaaggcgt gggaggacaa gggcgaccgt gcggtgctca ccgtcgagct
     3001 gaaggacgga aagtccgccg cgatcgacgc cgacaagatc ctgctgtcgg tcg
//
LOCUS       DASVOR010000523         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_326300,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000523 DASVOR010000000
VERSION     DASVOR010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326300"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(331..>1863)
                     /locus_tag="VF516_10510"
     CDS             complement(331..>1863)
                     /locus_tag="VF516_10510"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8108150.1"
                     /translation="AQARQFLTRFSAIDTTGFPDQEALNQQLMVRQLKERLDHAKFEG
                     WLMPIDQFGGLHIQVAALVPLLPFATVKDYDDYIARLHKLPAAFDQVTVLMKEGVAKH
                     LIPPRILLEQCVGQAEALAKGAPADSPFATPLKKFPAEIAKADQDRIRAAVLAAVKDQ
                     IQPTYKAFAKYLRDDYVPHGRKDVGMWALPDGDTRYAARVKQMTTTDLTADQIHEIGL
                     AEVARIEAAQAEIGKQLGFADFAAFKRHVRTNRKLYARSPEDILAHYRKYTDQMYTKL
                     PELFGRLPRQRMTIEPFEKFREKEAAGAQYDQGTPDGSRPGRVLVNTYEATKRLTIDM
                     ESTAYHEGVPGHHLQIAIQQELGELPPFRQQGGYVAFDEGWALYSERLGKEVGFYQDP
                     YNDYGRLQDEILRAIRLVVDTGLHAKHWTREQVVQFFHDHSTIEEPDVQAETNRYIAI
                     PGQALGYKIGQLTILRLRDKAQTALGKRFDIRAFHDEVLGAGAVPLDILERRIDAWIA
                     AR"
BASE COUNT          252 a          619 c          665 g          327 t
ORIGIN      
        1 aggatcccgc cgaggccgcc cccgccgccc aggccgccga acatgccgag gatgttgtcg
       61 gccagggcgc cccactgctg gcccttggcg ccgaacaggc cgccgatcat ggagccgatg
      121 ccgccgccgg tcaccggggc gagctcatcg accgagagag tctccaacgt ctgattcgtg
      181 gtggtcatcg tggtcggtgc ggctgcagcg accgtgccga caagcaggca aacgatttcg
      241 ccaggatcct gggggtgcaa gcgcctcctg cgggatgcag ctgtacgccg cgcgcggcag
      301 cttcgctttt gaccgcggcg gcggtcgcgc tcagcgcgcg gcgatccagg cgtcgatccg
      361 gcgctccagg atgtcgagcg gcaccgcccc ggcgccgagc acctcgtcgt ggaacgcgcg
      421 gatgtcgaac cgcttgccga gcgcggtctg ggccttgtcg cgcagccgca ggatcgtgag
      481 ctgcccgatc ttgtagccga gcgcctggcc ggggatcgcg atgtagcggt tggtctcggc
      541 ctggacgtcg ggctcctcga tcgtcgagtg gtcgtggaag aactggacga cctgctcgcg
      601 ggtccagtgc ttggcgtgca ggccggtgtc gaccaccagg cggatcgcgc gcaggatctc
      661 gtcctgcagc cggccgtagt cgttgtacgg atcctggtag aacccgacct ccttgccgag
      721 ccgctcggag tacagcgccc agccctcgtc gaacgcgacg tagccgccct gctggcggaa
      781 cggcggcagc tcaccgagct cctgctggat cgcgatctgc agatgatggc ccggcacgcc
      841 ctcgtggtac gcggtcgact ccatgtcgat ggtcagccgc ttggtcgcct cgtaggtgtt
      901 gaccagcacc ctgcccggcc gcgagccgtc gggagtgccc tgatcgtact gcgcaccggc
      961 agcctccttc tcgcggaact tctcgaacgg ctcgatcgtc atgcgctgcc tgggcagccg
     1021 gccgaacagc tcggggagct tggtgtacat ctggtcggtg tacttgcggt agtgggcgag
     1081 gatgtcctcg gggctcctgg cgtacagctt gcggttggtg cgcacgtggc gcttgaacgc
     1141 ggcgaagtcg gcgaacccga gctgcttgcc gatctcggcc tgcgcggcct cgatgcgcgc
     1201 gacctcggcc aggccgatct cgtggatctg gtcggcggtg agatcggtcg tggtcatctg
     1261 cttgacccgc gcggcgtagc gcgtgtcgcc gtcgggcagc gcccacatcc cgacgtcctt
     1321 gcggccgtgc ggcacgtagt cgtcgcgcag gtacttcgcg aacgccttgt acgtcggctg
     1381 gatctggtcc ttgaccgccg cgagcaccgc ggcgcggatc cggtcctggt cggccttggc
     1441 gatctcggca gggaacttct tcagcggcgt ggcgaacggg ctgtcggcag gcgctccctt
     1501 ggcgagcgcc tcggcctggc cgacgcactg ctcgagcagg atgcgcggcg ggatcaggtg
     1561 cttggcgacg ccctccttca tcagcacggt gacctggtcg aacgccgcgg ggagcttgtg
     1621 caggcgcgcg atgtagtcgt cgtagtcctt gacggtggcg aacggcaaca gcggcaccag
     1681 cgccgcgacc tggatgtgca ggcctccgaa ctgatcgatc ggcatcagcc agccctcgaa
     1741 cttcgcatgg tcgaggcgct ccttgagctg gcgcaccatg agctgctggt tgagcgcctc
     1801 ctggtcgggg aacccggtgg tgtcgatcgc cgagaaccgg gtcaggaact gccgggcctg
     1861 ggc
//
LOCUS       DASVOR010000524         5025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_209370,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000524 DASVOR010000000
VERSION     DASVOR010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209370"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VF516_10515"
     CDS             <1..719
                     /locus_tag="VF516_10515"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8108151.1"
                     /translation="LLALSACNIVSWNVLVIYGITLLPSGRAALLGYSMPLWSMILSV
                     WLLDERLTARRGLSLLLGMSGVAILLGDDLAGMAGATTGVVLMLAAAVSWALGTVLLK
                     RFELPIPTLSLTGWMMITGSVPIAAAAAALEHDRWRPITTVPALGLVYCVFISFMFCY
                     WAWNRLVLMVPVAVSSISALATPVVGIISGMWVLHEPLTWQEIAASAFILGAIALVLR
                     PSRPGAVREQPISVRSIQRG"
     gene            856..1368
                     /locus_tag="VF516_10520"
     CDS             856..1368
                     /locus_tag="VF516_10520"
                     /inference="COORDINATES: protein motif:HMM:NF018951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdC"
                     /protein_id="HEX8108152.1"
                     /translation="MPALVVHPPILLVVAVTLAGAVAMLTWRSHEARRVVTARSILIP
                     PIGMSTGFSMFAVPATRVPLTWALAALALGALVFAIPLVRTSRLVRQDDRILVQRSRA
                     FVAILLGLVAVRFGLRTWIEQYISPLQTGALFFLLAFGAIVRWRVAMLREYRRLTDVE
                     MLGSPDSTAA"
     gene            1522..1686
                     /locus_tag="VF516_10525"
     CDS             1522..1686
                     /locus_tag="VF516_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108153.1"
                     /translation="MFNIIEMATGWNLDLVIRRARPFSVEEMKRRVQMAGQTGEVHAR
                     DSRSMTHQTT"
     gene            1741..2262
                     /locus_tag="VF516_10530"
     CDS             1741..2262
                     /locus_tag="VF516_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108154.1"
                     /translation="MLRPLFALALLAATASTSGAAPATANCSAPEYHQLDFWLGDWDT
                     LDSDSKDRTTSIARTHVDPIAAGCAVHELYEQVDGLIGDSILSYDPVRKQWQQTWVTN
                     RGAIMAIYGNFKGGALILEGETHLRDGSTVHQRITWKAEGKGVRESAVMSKDGGKTWK
                     PAFDVLFVKHASS"
     gene            2397..2522
                     /locus_tag="VF516_10535"
     CDS             2397..2522
                     /locus_tag="VF516_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108155.1"
                     /translation="MAADAAAGGFQAILMVVVVPVVIPWGYVFRNYVKRPSDRWL"
     gene            complement(2551..>5025)
                     /locus_tag="VF516_10540"
     CDS             complement(2551..>5025)
                     /locus_tag="VF516_10540"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX8108156.1"
                     /translation="QRTGAPPEMPSTIVVTDASGAWSATAIAAGSYQVTASAVGFLPA
                     STSALAIAAGERNDHVDLALASGGTTGSGTVSDIGGGPIAGARVTMRPESIRGARSAT
                     DEVIALSGDDGSYQLTLPEGGCAARVTHDDYTNGSKWFRVAAAPLVVDFALVPGATIR
                     GQVIASDGAPVPGAEVTSRTDRRGFGGYGRATADADGRFTLKGIGAGALALVASGRGH
                     ASRNPTKITVGVGEQVDNVKIVVDRGFSISGTIIDASTRKAIAGVRVQGYAVPGGGAA
                     RSEPTGADGGFELTGLGRGSYLLSAEGDGVMPEYGKSAEVSDKDVTGIVIEMAAGVSL
                     SGRVDPPVATAVRIEADQDKIGRGNAMNAARAFGVAADSDPTTGAFVLHQVPPGEFSV
                     IATHADGRTGKLAVIVADKDQANLVVTLEPRASIAGKVIDASGAAVPGVRVAAEPEVP
                     GLPSRAARIVAPQRDAVITGADGSFQIVGLDAGTLELIVRDDQGSLSPAHGDKLAEVE
                     LTRGQALTGLVLTVEPRDGKITGRVIGSDGKPAADAWVTAAYQPSGANPVEARFARML
                     AGGLPALTSAEGRFALEHLHRGSYQLVVDGPRWTSRAEATAKVGDDVTITLAMLGTLS
                     GHVTAGGAPVKTYDLDCRIADARFSLPGGADAFEQHVSADDGSYSAEHIVPGSYVCTI
                     TSERGTARDPITIPSGPIQHDFALVPFASVTGTIVDATTGKPLAGLTVLNPNARGARS
                     FAAMISGNGQKTDASGRFELTSQPLGDGRLMVLDGQTPVMPAAGGSTRFTLTAGQRLD
                     LGTIKVTEIPRSTGDTVTITSHAPAR"
BASE COUNT          658 a         1795 c         1767 g          805 t
ORIGIN      
        1 cgttgctcgc gctgtcggcc tgcaacatcg tgagctggaa cgtcctggtg atctacggca
       61 tcaccctgtt gccgtcggga cgagccgcgc tgctcggcta ctccatgccg ctgtggagca
      121 tgatcctgtc ggtctggcta ctcgacgagc ggctcacggc gcggcgcggc ctctcgctgc
      181 tgctcgggat gagcggcgtc gcgatcctcc tcggggacga cctcgccggg atggcgggcg
      241 caaccaccgg cgtcgtgctc atgctcgccg ccgcggtctc gtgggcgctg ggtacggtgc
      301 tgctcaagcg cttcgagctg ccgatcccga cgctgtcctt gaccggctgg atgatgatca
      361 ccggcagcgt gccgatcgcc gcggccgcag cggccctgga gcatgaccgc tggcgaccca
      421 tcaccaccgt tcctgcgctc ggcctggtct actgcgtgtt catcagcttc atgttctgct
      481 actgggcgtg gaaccggctc gtcctcatgg tgccggtggc cgtctcatcg atctctgcgc
      541 tcgcgacgcc cgtggtcggc atcatcagcg gcatgtgggt gctccacgag ccgttgacct
      601 ggcaggagat cgccgcgagc gcgttcatcc tcggtgccat cgcgctcgtg ctccgcccga
      661 gccgtccggg cgcagtgcgc gagcaaccga tctccgtccg atccatccag cgcggatgac
      721 gttgaaccgg caggcccggc cacgcaacca ccgcgtagaa ggtgcggcgc cgcgcacgcc
      781 ctgcgcgatt tcgacgtggt cgggccccgg caggcgtatc cggtctggca cgcgcgatgc
      841 gaccaccgat ccgcgatgcc tgcgctggtc gtccatcctc cgatcctcct cgtcgttgcg
      901 gtgaccctcg ccggcgcggt cgccatgctg acgtggcgtt cgcacgaggc ccgtcgcgtg
      961 gtgaccgccc gctcgatcct catcccaccg atcggcatga gcaccggctt ctcgatgttt
     1021 gccgtgccgg cgacgcgggt cccgctcacc tgggcgctgg ccgcgctcgc gctcggcgcg
     1081 ctggtgttcg cgatcccact cgtccgcacg tcgcggctgg tccggcagga cgatcggatc
     1141 ctcgtccagc gatcgcgggc cttcgtcgcg atcctgctcg ggctcgtcgc ggtccgattc
     1201 ggcctgcgca cctggatcga gcagtacatc tcgccgctcc agaccggcgc gctgttcttc
     1261 ctgctcgcgt tcggcgcgat cgtgcgctgg cgcgtggcga tgctgcgcga gtaccgacgg
     1321 ctgaccgatg tcgagatgct cggttcgccc gactcgacag cggcgtgagg cgcgccccac
     1381 ggggagccgc ggaccacgcg cgatctcgat ctggtcatcg acccgagccc ggaccagctg
     1441 aacgagctga tcggggcgct ggatcccgag cgcttccatg tcgaccctaa tgtcgcgcgc
     1501 gatgcgctcc ggcgtcggtc gatgttcaac atcatcgaga tggcgacggg ctggaacctc
     1561 gatctcgtga tccggagggc ccgcccgttc tcggtcgagg agatgaaacg ccgcgtgcag
     1621 atggcgggcc agaccggcga ggtgcacgcg cgggattcga ggtcgatgac gcaccagacg
     1681 acgtagcgga cgacgcggct cgcgtgcttc actccgggat ctcaaccagg agatgccgcc
     1741 atgctgagac cgctgttcgc cctcgccctg ctggccgcca ccgcttccac cagtggcgcc
     1801 gcaccagcta ccgccaactg ctcggctccg gaataccatc agctggattt ctggctgggt
     1861 gattgggaca ccctcgattc ggactcgaag gatcgcacga cctccatcgc ccgcacccac
     1921 gtggacccga tcgccgccgg ctgcgccgtg cacgaactct acgaacaggt cgatgggctc
     1981 atcggcgaca gcatcctcag ctacgacccg gtccggaagc agtggcagca gacctgggtc
     2041 accaaccggg gcgccatcat ggcgatctac ggcaacttca agggtggcgc gctgatcctc
     2101 gagggcgaga cgcacctccg ggatgggagc acggtccacc agcgcattac ctggaaagcc
     2161 gaaggcaagg gcgtgcgcga gtcggcggtc atgtcgaagg acggcggtaa gacctggaaa
     2221 ccggccttcg acgtgctgtt cgtaaagcac gccagttcct gacttcagct tccgacgagg
     2281 acgcctcgtc gcgctcgcgg ccgcgacgcc gagctcagca gctgcaccac gagccaatct
     2341 tagcggcgcc ccaagtagaa tcctcctggc ggcaggatcg cgagccaccc ggtcgcgtgg
     2401 ccgctgatgc ggcggcgggc ggattccagg cgatcctgat ggtggtcgtc gtgcctgtcg
     2461 tcatcccatg gggatacgtg tttcgcaatt acgtgaagcg tccgtccgac cgctggctct
     2521 gagtggagca ttcgcgcgca caggacgggg tcagcgcgct ggcgcgtggc tggtgatggt
     2581 gacggtatcg ccggtcgacc gcggtatctc ggtgaccttg atcgtcccga ggtcgagccg
     2641 ctggccggcg gtgagcgtga accgcgtcga gccgccggcc gcgggcatca ccggcgtctg
     2701 gccgtcgagc accatcaggc ggccgtcgcc gagcggctgg ctggtgagct cgaaccgccc
     2761 gctcgcgtcg gtcttctgcc cgttgccgga gatcatcgcc gcgaacgagc gcgcgccgcg
     2821 tgcgttcggg ttgagcaccg tgaggccggc gagcggcttg ccggtggtgg cgtcgacgat
     2881 cgtcccggtg accgaggcga acgggaccag cgcgaagtcg tgctggatcg gcccgctcgg
     2941 gatggtgatc gggtcccgcg cggtgccgcg ctcgctggtg atcgtgcaga cgtagctgcc
     3001 gggcacgatg tgctcggcgg agtagctgcc gtcgtcggcg ctgacgtgct gctcgaacgc
     3061 gtcggcgccg ccggggagcg agaaccgcgc gtccgcgatg cggcagtcga ggtcgtaggt
     3121 cttgaccggc gcgccgccgg ccgtcacgtg tccggacagc gtgcccagca tcgcgagcgt
     3181 gatcgtgacg tcgtcaccga ccttcgccgt ggcctcggcg cgcgacgtcc agcgcgggcc
     3241 gtcgaccacc agctggtagc tgccgcggtg caggtgctcg agcgcgaagc ggccctcggc
     3301 gctggtcagc gcgggcaggc cgccggcgag catccgggcg aaccgcgcct cgacggggtt
     3361 cgcgccgctg ggctggtacg ccgcggtcac ccaggcgtcg gcggcgggct tgccatcgga
     3421 gccgatcacc cggccggtga tcttgccgtc gcgcggctcg acggtgagca cgagcccggt
     3481 cagcgcctgg ccccgggtca gctcgacctc ggcgagcttg tcgccgtgcg cgggcgagag
     3541 gctgccctga tcatcgcgaa cgatcagctc gagcgtgccg gcatcgagcc cgacgatctg
     3601 gaacgagcca tcggcgccgg tgatcaccgc gtcacgctgg ggggccacga tccgcgccgc
     3661 gcggctcggc aggcccggga cctcgggttc ggcggcgact cgcacgcccg gcaccgcggc
     3721 tccactggcg tcgatgacct tgccggcgat cgacgcgcgc ggctcgagcg tgaccacgag
     3781 gttggcctgg tccttgtcgg cgacgatgac cgcgagcttg ccggtccggc cgtcggcgtg
     3841 ggtagcgatc accgagaact cgccgggcgg gacctggtgg agcacgaacg cgccggtggt
     3901 cggatccgag tctgcggcca ccccgaaggc cctcgctgcg ttcatcgcat tgccgcggcc
     3961 gatcttgtcc tggtcggcct cgatccggac cgcggtggcg accggcgggt cgacgcggcc
     4021 cgacaggctg accccggcgg ccatctcgat cacgatgccg gtgacgtcct tgtcgctgac
     4081 ctcggcggac ttgccgtact ccggcatcac gccgtcgccc tccgccgaca gcaggtagct
     4141 gccgcggccg aggccggtga gctcgaaccc gccgtcggcg ccggtcggct cggagcgcgc
     4201 cgcaccaccg cccgggaccg cgtacccctg gacgcgcacc cccgcgatcg ccttgcgggt
     4261 cgaggcgtcg atgatggtgc ccgagatcga gaacccgcga tcgacgacga tcttgacgtt
     4321 gtcgacctgc tcgccgaccc cgaccgtgat cttcgtcggg ttccgcgatg cgtggccgcg
     4381 gcccgacgcg acgagcgcga gcgcgcccgc gccgatgccc ttgagcgtga acctgccgtc
     4441 ggcgtcggcg gtcgcgcggc cgtagccgcc gaacccgcgc cggtcggtgc gcgaggtgac
     4501 ctcggcgccc ggcaccggcg cgccatcgct cgcgatcacc tggccgcgga tcgtcgcgcc
     4561 gggcacgagc gcgaaatcga ccacgagcgg cgcggcggcg acccggaacc acttgctgcc
     4621 gttcgtgtaa tcgtcgtggg tgacgcgcgc cgcgcagccg ccctcgggga gcgtcagctg
     4681 gtagctgccg tcgtcccccg acagcgcgat cacctcgtcg gtcgcgctgc gcgcgccccg
     4741 gatcgactcg gggcgcatcg tcacccgtgc gccggcgatc gggccgccgc cgatgtcgct
     4801 cacggtgccg gacccggtcg tgccccccga cgcgagcgcg agatcgacgt gatcgttgcg
     4861 ctcgcccgcc gcgatcgcga gcgcgctcgt cgatgccggc aggaagccca ccgcgctcgc
     4921 cgtcacctgg tagctgccgg cggcgatcgc ggtcgcgctc catgcgccgc tcgcgtcggt
     4981 caccacgatc gtgctgggca tctcgggcgg cgcgcccgtc cgctg
//
LOCUS       DASVOR010000525         2094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_121687,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000525 DASVOR010000000
VERSION     DASVOR010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121687"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..821)
                     /locus_tag="VF516_10545"
     CDS             complement(3..821)
                     /locus_tag="VF516_10545"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEX8108157.1"
                     /translation="MSSERPEATRILGLSMNTHLQVALSGAIPALILMWLVDRLDAKR
                     PEPRGTRWLVALAGMLSVIPALILEVMIPALTAGQIEPEMTYQGSSFQAFIVAAGVEE
                     ACKILLVYWVVWRRPEFDERMDGIVYASRGGLGFALFENVLYLLRQNSLQGQIIVWVE
                     RALLAVPGHAMWTGMIGAMAARRRFDGTGLGLFGGYLLAVAFHGAYDLSVFLQQPLHL
                     EGRETASRILLVVPVALTVVAFLVLRSMARTALRLDDAEAVQAAARSALPPRIG"
     gene            850..>2094
                     /locus_tag="VF516_10550"
     CDS             850..>2094
                     /locus_tag="VF516_10550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108158.1"
                     /translation="MAPPEPTTADPMRIGRYEVISHLATGGMAQIYLARQTGLGSFER
                     HVVLKTILRERAADQRFVTMFLDEAKLAATLNHQNVAQVYEVDHADGAYFMAMEYVHG
                     ENTRAILETTIRRGWTIPLELGVMIISGAAAGLHHAHERRGKNGQPLQIVHRDVSPAN
                     VMVGYDGSIKVLDFGIAKAEERATKTIGGTIKGKYGYMSPEQCKGKPIDRRSDIFALG
                     IVLYELSTLRRAFKGNDDFETMKRIVAGDLVLPSTLVPGFPRELEAIILTALASDPGA
                     RFQTAQELIEALDAFTVRAKLTGSNTAMSRFMGQLFGTKREPWIDTGLQSTDHTLVTP
                     AEISELDDDDNDETLVVTGRQEVQHALEQLNLSPPPAVARTPSPVIAAPPAAAPPTEP
                     QLRPPQGMERARRPSVRRTGERP"
BASE COUNT          366 a          759 c          708 g          261 t
ORIGIN      
        1 gctcagccga tccgcggcgg cagggcggac cgcgccgcgg cctgcaccgc ctcggcgtcg
       61 tcgagccgca gcgcggtgcg ggccatcgac cgcaggacca ggaacgccac cacggtcagc
      121 gccaccggga ccacgagcag gatgcgcgac gcggtctcgc ggccttccag gtgcagcggc
      181 tgctggagga acaccgacag gtcgtacgcg ccgtgaaacg cgacggcgag caggtagccg
      241 ccgaacaggc ccaggccggt gccgtcgaac cgccgccgcg cggccatcgc gccgatcatc
      301 ccggtccaca tcgcgtgccc cgggaccgcc agcagtgcgc gttcgaccca cacgatgatc
      361 tggccctgca gcgagttctg ccggagcaag tacaggacgt tctcgaacag cgcgaacccc
      421 agcccgccgc ggctggcgta cacgatgccg tccatccgct cgtcgaactc cggccgccgc
      481 cacaccaccc agtacaccag caggatcttg cacgcctcct cgacccccgc cgcgacgatg
      541 aaggcctgga agctggagcc ctgataggtc atctccggct cgatctggcc ggcggtcagc
      601 gccgggatca tgacctcgag gatcagtgcg gggatcaccg acagcatgcc cgccagcgcg
      661 acgagccagc gcgtgccgcg tggttcgggg cgcttggcgt cgagccggtc gacgagccac
      721 atcaggatca gcgcggggat cgcaccggac agcgcgacct gaagatgggt gttcatcgac
      781 aagcccagta tacgcgtggc ctcgggccgc tcgcttgaca cgctatgcca ggcagctgat
      841 gctccccagg tggcgccgcc ggagcccacg accgccgatc ctatgagaat cggccgctac
      901 gaggtgatca gtcacctcgc gaccggcggc atggcgcaga tctacctggc gcgccagacc
      961 ggactgggct cgttcgagcg ccacgtcgtg ctcaagacca tcctgcggga gcgcgcggcg
     1021 gaccagcgat tcgtcacgat gtttctggac gaggccaagc tcgccgcgac cctgaaccac
     1081 cagaacgtcg cgcaggtcta cgaggtcgac cacgccgacg gcgcgtactt catggcgatg
     1141 gagtacgtgc acggcgagaa cacgcgggcg atcctcgaga ccacgatccg ccgcggctgg
     1201 acgatcccgc tcgagctcgg ggtcatgatc atcagcggcg cggcggccgg cctgcaccac
     1261 gcccacgagc gccggggcaa gaacggtcag ccgctgcaga tcgtccaccg cgacgtgtcg
     1321 ccggccaacg tcatggtcgg ctacgacggc agcatcaagg tcctcgattt cgggatcgcc
     1381 aaggccgagg agcgcgcgac caagacgatc ggcgggacga tcaagggcaa gtacggctac
     1441 atgtcgcccg agcagtgcaa gggcaagccg atcgaccggc ggagcgacat cttcgcgctc
     1501 ggcatcgtgc tctacgagct gtcgaccctg cgccgggcgt tcaagggcaa cgacgacttc
     1561 gagaccatga agcggatcgt cgccggcgat ctcgtgctgc cgtcgacgct ggtgcccggc
     1621 ttcccgcgcg agctcgaggc gatcatcctg accgcgctgg ccagcgatcc gggcgcgcgg
     1681 ttccagaccg cccaggagct gatcgaggcg ctcgacgcgt tcaccgtgcg ggccaagctg
     1741 accggctcga acaccgcgat gagccggttc atgggccagc tgttcggcac caagagggag
     1801 ccctggatcg acaccggcct gcagagcacc gaccacacgc tggtcacgcc ggccgagatc
     1861 agcgagctcg acgacgatga taacgacgag acgctggtgg tgaccggccg ccaggaggtc
     1921 cagcacgcgc tggagcagct gaacctctcg ccgccaccgg ccgtcgcgcg cacgccatcg
     1981 cccgtgatcg ctgcgccgcc cgccgccgca ccgccgaccg agccgcagct gcggccgccc
     2041 cagggcatgg agcgcgcccg gcggccgagc gtgcgccgga ccggcgagcg gccc
//
LOCUS       DASVOR010000526         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238454,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000526 DASVOR010000000
VERSION     DASVOR010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238454"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF516_10555"
     CDS             <1..543
                     /locus_tag="VF516_10555"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEX8108159.1"
                     /translation="PGSGAGGVLAEMAGALRGAGAEVLAMPCNTAHAHADVIQRASGL
                     PLIDMIDLGAEAAGQAGAMRVGVLGTKGAQRLYREYLAARAMGMVVLPPERQEQFMVT
                     LYRIKSGDLGADVKREMQGHAEARRADGAEVLIAGCTEVPLVLTNDDVKLQLIDPGDL
                     LARRAVGVCLGWEPLPHVPA"
     gene            complement(547..1446)
                     /locus_tag="VF516_10560"
     CDS             complement(547..1446)
                     /locus_tag="VF516_10560"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX8108160.1"
                     /translation="MTFNDDASLDTSQVGDRRGIGGGGLAIGGGGLGIVGLIIYLLIS
                     VLGGGNSSDSGTGVQTPFQPGETGAVSQQDLAQVCRTGADANSRDDCRIVAYTNSVQQ
                     YWSRAMGSRYQPAETIIFSGGTQTGCGNATSDVGPFYCPADGKAYFDLGFFDELRTRF
                     GAQGGSFAQGYVVAHEYGHHIQNLLGTSDRVGGGTGPESGSVRLELQADCYAGVWAKN
                     ATTTTGANGRPLFESVTQSDINNALDAAAAVGDDRIQARAQGRVTPESFTHGTSAQRT
                     RWFKRGADSGDPNACDTFGASSL"
     gene            complement(1443..>1772)
                     /locus_tag="VF516_10565"
     CDS             complement(1443..>1772)
                     /locus_tag="VF516_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108161.1"
                     /translation="LVAALCDGNRVALARVLDETLAELAGARDALAGEGSVLPLATAG
                     HQARWAWSEAAPAVGVTLDRDTPNLRDDLLALGAAGGTLDAVTSHQLHGRRPDVSGSP
                     GQGRLRP"
BASE COUNT          225 a          647 c          653 g          247 t
ORIGIN      
        1 cctggctcgg gcgccggcgg cgtgctggcc gagatggcgg gcgcgctgcg cggcgccggc
       61 gccgaggtgc tggccatgcc ctgcaacacc gcccacgccc acgccgacgt catccagcgg
      121 gcctcgggcc tgccgctgat cgacatgatc gacctgggcg ccgaggcggc gggccaagcc
      181 ggggcgatgc gcgtcggcgt gctgggcacc aagggggcgc aacggctcta ccgcgagtac
      241 ctcgccgccc gcgccatggg catggtcgtc ctgccgcccg aacggcaaga gcagttcatg
      301 gtcaccctct accggatcaa gtccggagac ctgggcgccg acgtgaagcg cgagatgcag
      361 ggccatgccg aggcccggcg cgccgatggg gccgaggtgc tgatcgccgg ctgcaccgaa
      421 gtgccgctcg tgctcaccaa cgacgacgtg aagctgcagc tgatcgaccc cggcgacctg
      481 ctggcccgcc gcgcggtggg cgtgtgcctg ggctgggagc cgctgccgca cgtgccggcg
      541 tagcggctac aagctcgacg ccccgaacgt gtcgcaggcg ttcggatcgc cgctgtctgc
      601 gccgcgcttg aaccagcggg tccgctgggc cgacgtgccg tgggtgaagc tctcgggcgt
      661 cacccggccc tgcgcgcggg cctggatccg gtcgtcgccg accgcggccg ccgcgtccag
      721 cgcgttgttg atgtccgact gggtgaccga ctcgaacagc ggccggccgt tggcgccggt
      781 ggtcgtcgtc gcgttcttcg cccagacgcc ggcgtagcag tcggcctgca gctccagccg
      841 caccgatccg ctctccggac cggtcccgcc gccgacccgg tcggaggtgc cgagcaggtt
      901 ctggatgtgg tgtccgtact cgtgtgcgac cacgtatccc tgtgcgaacg agccgccctg
      961 ggcgccgaac cgggtccgca gctcgtcgaa gaaaccgagg tcgaagtagg ccttgccgtc
     1021 ggccgggcag tagaacgggc cgacgtcgct ggtcgcgttg ccgcagccgg tctgggtgcc
     1081 gccggagaag atgatcgtct ccgccggctg gtagcgcgaa cccatggcgc gcgaccagta
     1141 ctgctgcacg ctgttggtgt acgcgacgat ccggcagtcg tcccgggagt tcgcgtcggc
     1201 gccggtccgg cacacctggg cgagatcctg ctgcgagacc gcaccggtct cgcccggctg
     1261 gaacggggtc tggaccccgg tgccgctgtc ggacgagttg ccgccgccca ggaccgagat
     1321 cagcaggtag atgatgaggc cgacgatgcc tagcccgccg ccgccgatgg ccagcccgcc
     1381 gccgccgatg ccgcggcgat cgccgacctg ggaggtgtcc aggcttgcgt cgtcgttgaa
     1441 cgtcacggcc ggagccttcc ctgcccgggg cttcccgaca cgtccgggcg ccggccgtgc
     1501 agctggtgcg aggtcacggc gtccagagtg ccgccggccg cgcccagtgc caacaggtcg
     1561 tcgcgcaggt tcggcgtgtc gcggtccagc gtcaccccga ccgccggcgc cgcctcggac
     1621 caggcccacc gcgcctggtg gccggcggtg gcgagcggca gcacggagcc ttctccggcg
     1681 agcgcgtccc tggcgccggc cagctcggcc aacgtctcgt ccagcacccg ggcgagcgcg
     1741 acccggttgc cgtcgcagag cgccgcgacc ag
//
LOCUS       DASVOR010000527         2883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_63276,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000527 DASVOR010000000
VERSION     DASVOR010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63276"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VF516_10570"
     CDS             <1..311
                     /locus_tag="VF516_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8108162.1"
                     /translation="LRRCPRVHLKAVVVDGELLYLGSANWTGAGLGARGAGRRNFELG
                     VVTDDGPLLDQVQSMYDRIWTGGECAACKLRDDCPGPLSELAGEATGPPASRPRRRSR
                     "
     gene            complement(308..1753)
                     /locus_tag="VF516_10575"
     CDS             complement(308..1753)
                     /locus_tag="VF516_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108163.1"
                     /translation="METAVPQRTVGLDDRLAACAADPRVVAGAVSVDICVGADVFLRE
                     QFGGNGRTCATCHRVDNNFTIDPAFISKLPHNDPLFVAELDPALAGLEISAQMRSKSL
                     ILENVDGFDPDPRTHFVLRSVPHNFSMGTSVTRAPGDPVTTPAERTGWSGDGAPGGGT
                     LRDFLTGAITQHYTRTLARVPGTDFRLADDGELDRVDLFMRQLGRTNELALASVVMSD
                     AGAEVGRTTFIAVGCNLCHANAGANAAFGGGGNRNFDTGVESSRNADLRAFPHDGGFL
                     RDPANADGSFGDGTFNTPPLIEAADTGPFFHTATTVAGAPAHNTAIATVIEEAIAFYA
                     TPAFNNSPAGKILPISLTAEQIDDIGRFLRGINAAFNAALAVKRLDASAALVKQFPNT
                     YLEIQREELRLAKVEIRDAIRVLHGVPNLDAASLAQLKAAAALVDTARRTHMPATRAK
                     NISAARGLVSDASTRIGTNLTFQIGDGTVMF"
     gene            2248..>2883
                     /locus_tag="VF516_10580"
     CDS             2248..>2883
                     /locus_tag="VF516_10580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8108164.1"
                     /translation="MTAVLYVEDVLLVDRCLTGEPAATRELFRRHKSRVHACLFRVLG
                     GNRDMDDLLQEAFLQVFQSLRSWRAEASLATWIDRVAVRCAYRHLAQRRRRIATDLLD
                     DDAAAGAATAQEAGPGARRQLARDGVARLYAVLDELSPAARLAFTLHELDGRSLAETA
                     ELVGSTVTATKLRVWRARKRIEAAAAADPVLREFIAPAGGQVIPMDEPRDAT"
BASE COUNT          445 a          988 c         1025 g          425 t
ORIGIN      
        1 cgctgcggcg ctgcccgcgc gtccacctca aggcggtggt cgtcgacggc gagctcctgt
       61 acctgggcag cgcgaactgg accggcgccg ggctcggcgc gcgcggcgcc ggccgccgca
      121 acttcgagct cggcgtggtc accgacgacg gcccgctgct cgatcaggtc cagtcgatgt
      181 acgaccggat ctggaccggc ggcgagtgcg ccgcgtgcaa gctgcgcgac gactgccccg
      241 gcccgctgtc cgagctcgcg ggcgaggcca ccgggccacc ggcgagccgg ccgcgccgcc
      301 gctcgcgcta gaacatcacc gtgccatcgc cgatctggaa ggtcaggttg gtgccgatcc
      361 gggtcgatgc atcggacacc agcccgcggg cggcgctgat attcttcgcc cgggtcgccg
      421 gcatgtgggt ccgccgcgcg gtgtcgacca gcgccgccgc ggccttcagc tgggccagcg
      481 acgcggcgtc gaggttcggc acgccatgga gcacgcggat cgcgtcgcgg atctcgacct
      541 tggccagccg cagctcctcg cgctggatct cgaggtaggt gttcgggaac tgcttcacca
      601 gcgccgcgga tgcgtcgagc cgcttgaccg cgagcgccgc gttgaacgcc gcgttgatcc
      661 cgcgcaggaa ccggccgatg tcatcgatct gctccgcggt gagcgagatc ggcaggatct
      721 tgccggccgg cgagttgttg aacgccggcg tggcgtagaa cgcgatcgcc tcctcgatca
      781 cggtggcgat cgcggtgttg tgcgccggag cgccagcgac cgtcgtggcc gtgtggaaaa
      841 atgggccggt gtccgcggcc tcgatcagcg gcggggtgtt gaacgtaccg tcgccgaacg
      901 agccgtccgc gttcgccgga tcgcggagga agccgccatc gtgcggaaac gcgcggagat
      961 ccgcattacg agaagattct acacctgtat cgaaattgcg attcccgccg ccgccgaaag
     1021 ccgcattggc tccggcgttg gcgtggcaca gattgcagcc gactgcgatg aaggtggtcc
     1081 ggcccacctc ggcgccggcg tcggacatca cgacgctggc gagcgcgagc tcgttggtcc
     1141 ggccgagctg gcgcatgaac aggtcgaccc ggtcgagctc gccgtcgtcg gcgagccgga
     1201 agtcggtgcc ggggacgcga gcgagcgtcc gcgtataatg ctgtgtgatc gcgccggtca
     1261 ggaagtcgcg cagggtcccc ccgccgggcg cgccgtcgcc ggaccatccg gtgcgctcgg
     1321 ccggcgtggt caccggatcg cccggcgcac gggtcacgct ggtgcccatc gagaagttgt
     1381 gcggcacact gcgcagcacg aagtgggtgc gcgggtccgg atcgaagcca tcgacattct
     1441 cgaggatcag gctcttggac cgcatctgcg ccgagatctc caggccggcg agagcagggt
     1501 cgagctcggc cacgaacagc ggatcgttgt gtggtagctt ggagatgaac gccggatcga
     1561 tcgtgaagtt gttgtcgacc cggtggcagg tcgcgcaggt tcgtccgttg ccgccgaact
     1621 gttcgcgcag gaacacatcg gcgccgacgc agatatcgac gctcaccgcg ccggcgacga
     1681 cccgagggtc cgcggcgcac gccgcgagcc gatcatccag gcccaccgtc cgctggggta
     1741 ctgccgtctc catcgcatcc cccgcgacgc accccgcccc gacccatagc cccatcaacg
     1801 cacacccgca gcgtgagatc atggaatccc tcccatctgt ttcagaaacc tgggattcga
     1861 acatccgtat tgatcattga aatgaccgtc tacggtaata cgaaattcac tctacaagtg
     1921 attatggcaa accgcacagg gtaatatgtg gcagtcacag ctcgcggaag gtgtgcattg
     1981 cgagcgtgcg tgggcgctcg cggtgccgcg ccggacttgc gcggagcgga gccaccgcgg
     2041 tgccgcggcc gcgacaggca gacatcgctc acgcgacggc gcccggcaac ggccgcgcgg
     2101 cgaagcaagg aacaaaccgc ccgggcagag cgtcccacag acagcccggc cgcacccggg
     2161 tttcgacctc gatgacaaca ttcaatggat ctgatgattt acgacggcgc gtaaccgcgg
     2221 gcggcacacc gggcactatg tagggacgtg accgcggtgc tgtacgtcga agacgtcttg
     2281 ctggtcgacc gctgcctgac cggcgagccc gcggcgaccc gcgagctgtt ccgccgccac
     2341 aagagccggg tccacgcctg cctgttccgc gtgctgggcg gaaaccgcga catggacgac
     2401 ctgctgcagg aggcgttcct ccaggtgttc cagtcgctgc gcagctggcg cgccgaggcc
     2461 agcctcgcga cctggatcga ccgcgtcgcg gtgcgctgcg cgtatcgcca cctcgcgcag
     2521 cgccggcgcc ggatcgcgac cgatttgctc gacgacgacg cggcggcggg cgcggcgacc
     2581 gcgcaggagg ccggaccggg tgcgcggcgc cagctggcgc gcgacggcgt ggcgcggctg
     2641 tacgcggtgc tcgacgagct gtcgccggcc gcccggctcg cgttcacgct gcacgagctc
     2701 gacggccggt cgctcgccga gacggcagag ctggtcggct cgacggtgac cgcgaccaag
     2761 ctgcgggtgt ggcgcgcgcg caagcggatc gaggccgccg ccgccgccga tccggtgctg
     2821 cgcgagttca tcgcgccggc cggcggccag gtcatcccga tggatgagcc gcgcgacgcg
     2881 acg
//
LOCUS       DASVOR010000528         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413415,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000528 DASVOR010000000
VERSION     DASVOR010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413415"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..665)
                     /locus_tag="VF516_10585"
     CDS             complement(39..665)
                     /locus_tag="VF516_10585"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8108165.1"
                     /translation="MTLPLRIAVVCYPALGGSGVIASELALGLAGRGHRVTLVATALP
                     ERLRGTAVRYERVEVPSSPVFEHAPYGEAVASRLVELARREPIDLIHLHYAVPHAASA
                     LLAQAVLGRAAPAMVVTLHGTDVTALGSHPSLHAVTAAALDACAGLTTPSRYLRGEAV
                     DHFALPPERIEVIANFVDLERFRPPPRREPAQVDALFGEPAGQQGQAA"
     gene            complement(662..1414)
                     /gene="bshB1"
                     /locus_tag="VF516_10590"
     CDS             complement(662..1414)
                     /gene="bshB1"
                     /locus_tag="VF516_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEX8108166.1"
                     /translation="MLAFGPHPDDVELFCGGTLIRLAELGHTIAVCDLTRGEKASHGT
                     PEERARETAAASAELGLAFRDQLGLPDTELAATPAQIMRVVEVLRHRRPEIVLAPWIE
                     DRHPDHAAAGALVARAVMFAGVRNYAPEAGPRFVPRQLLYYAMRHRMPPSFIVDTSAA
                     AARKARAIACYGSQLQRRAGAGPGDADPTLISAPGAIAAIEARDRYYGSMIGTSHGEP
                     LRSPATLGLVDPVRHFRDNPFTEAHAFEPLVP"
BASE COUNT          199 a          577 c          607 g          191 t
ORIGIN      
        1 tgctcgcgcc gcatcgccag cgcgacgcgc tcgaacacct aggccgcctg gccctgctgc
       61 ccggccggct cgccaaacag tgcgtcgacc tgcgccggct cgcggcgcgg tggcggccgg
      121 aaccgctcga ggtcgacgaa gttcgcgatc acctcgatcc gctcgggcgg cagggcgaag
      181 tgatcgaccg cctcgccgcg caggtagcgc gacggcgtgg tcagcccggc gcacgcgtcg
      241 agcgcggccg cggtcactgc gtgcaggctg gggtgcgagc cgagcgcggt cacgtcggtg
      301 ccgtgcagcg tgaccaccat cgccggcgcg gcgcggccga gcaccgcctg tgcgagcagc
      361 gcgctggcgg cgtgcggcac cgcgtagtgc aggtggatca ggtcgatcgg ctcgcggcgc
      421 gcgagctcga ccaggcggct cgccaccgcc tcgccgtacg gcgcgtgctc gaacaccggc
      481 gagctcggca cctcgacccg ctcgtagcgc accgcggtcc cgcgcagccg ctcgggcagc
      541 gcggtcgcga ccagggtgac gcggtggccg cggccggcga ggccgagcgc cagctcggag
      601 gcgatcacgc cgctgccccc cagcgcgggg tagcacacga cggcgatgcg aagcggtagg
      661 gtcatggcac caggggctcg aaggcgtgcg cctcggtgaa gggattgtcg cggaagtggc
      721 gcaccgggtc gaccagaccg agcgtggccg ggctgcgcag cggctcgccg tggctggtgc
      781 cgatcatgct gccgtagtag cgatcgcgcg cctcgatcgc cgcgatcgcg ccgggcgcgc
      841 tgatcagcgt cggatccgcg tcgccggggc cggcgccggc gcggcgctgg agctggcttc
      901 cgtagcacgc gatcgcgcgc gccttgcggg cggcggcggc cgatgtatcg acgatgaagc
      961 tcggcggcat gcggtggcgc atcgcgtagt acaggagctg gcgcggcacg aaccgcggcc
     1021 cggcctcggg cgcgtagttg cggaccccgg cgaacatcac cgcgcgggcg accagcgccc
     1081 cggccgcggc gtgatccggg tggcggtcct cgatccacgg cgcgagcacg atctcgggcc
     1141 ggcggtggcg cagcacctcg acgacccgca tgatctgcgc cggcgtcgcc gcgagctcgg
     1201 tgtcgggcag gccgagctgg tcgcggaacg caaggccgag ctcggcgctc gccgccgcgg
     1261 tctcccgcgc gcgctcctcg ggcgtgccat ggctcgcctt ctcgccgcgc gtcaggtcgc
     1321 acaccgcaat ggtgtggccg agctcggcga gccggatcag cgtgccgccg cagaacagct
     1381 cgacgtcgtc ggggtggggc ccgaacgcga gcacatcgac gccatatccc gtcatgccag
     1441 ctcccgcagg gtgaaccatc gcgccggatc cgaaggatcg atcgccgcga gcacgcgctc
     1501 gacgatcgcg cggtcgcccg tccgcgacgc gaacccggcg agcccgtgcg tgcgctcctg
     1561 gggcgtgccg tccg
//
LOCUS       DASVOR010000529         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238508,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000529 DASVOR010000000
VERSION     DASVOR010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238508"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VF516_10595"
     CDS             complement(<1..494)
                     /locus_tag="VF516_10595"
                     /inference="COORDINATES: protein motif:HMM:NF025001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEX8108167.1"
                     /translation="MISLAIVSPKGGVGKTTLCLNLAFAFAQLGRKTLLIDTDPQGGI
                     GHSLSGKARDARGLVDVTAGEPADRVVIQTRNPNLSILPVGQPPWDQLSSWAAKMADP
                     GFLAMHMEPLEPDYEIAILDTPAGLSGATYGVLSYASHILVPIQTEPLAIRVVGQLME
                     VLAHL"
     gene            complement(491..1315)
                     /locus_tag="VF516_10600"
     CDS             complement(491..1315)
                     /locus_tag="VF516_10600"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8108168.1"
                     /translation="MEGRREAAPAGWPGNEHRPASRSDTASAREATAKVVMEGDLSLL
                     SIPDLLQMICLGGYSRDIHLFDGPVQLGVIAIRHGCVERCFGFGTWGEAAFYKLVNLR
                     RGRYKVNEAQDMGGADATLTAYSWQALLMEAARLQDEAERVVQSATVTGRVIAFPTPA
                     SHAAAVDLPAPPPVVMAHAAPTATVSPIALPHIPPPASAAPTRRVSQTMELPAIEEDG
                     PRPTADFAHLLEQATASYLRRDLARAEELLQQCLALRPGDKRVLQNLERVRRKKSP"
BASE COUNT          248 a          564 c          561 g          244 t
ORIGIN      
        1 aggtgggcga gcacctccat gagctgaccg accacgcgga tcgccagcgg ctcggtctgg
       61 atcggcacga ggatgtggct ggcgtacgac agcacgccgt aggtcgcgcc cgacagcccg
      121 gccggcgtgt cgaggatcgc gatctcgtag tcgggctcga gcggctccat gtgcatggcg
      181 aggaagccgg gatccgccat cttcgccgcc cagctcgaca gctggtccca cggcggctgg
      241 ccgaccggca ggatcgacag gttcgggttg cgggtctgga tcaccacgcg gtcggcgggc
      301 tcgccggcgg tgacatcgac caggccgcgc gcgtccctgg ccttgccgga cagcgagtga
      361 ccgattccgc cctgcggatc ggtgtcgatg agcagcgtct tgcggccgag ctgggcgaat
      421 gcaaatgcga gattcagaca cagcgtcgtt ttccccacgc cgcctttggg actgacgatg
      481 gcaagcgaga tcatggcgat ttctttcgtc gaacgcgttc gaggttctgc agcacgcgct
      541 tgtcgcccgg ccgcaaggcg agacactgtt ggagaagctc ctcggcgcgc gccaggtccc
      601 ggcgcaggta gctcgcggtc gcctgttcca ggaggtgcgc gaagtctgcc gtcggtctcg
      661 ggccgtcctc ctcgatcgcg ggcagctcca tcgtctgcga cacccggcgc gtcggcgcgg
      721 cggacgccgg gggcggaatg tgcggcagcg cgatcggcga aacggtcgcc gtcggcgcgg
      781 cgtgcgccat cacgaccggc ggcggcgcgg ggaggtcgac cgcggcggcg tgcgaggccg
      841 gcgtggggaa cgcgatcacg cggccggtca ccgtcgccga ttgcacgacg cgctcggcct
      901 cgtcctgcag gcgcgcggcc tccatcagca gcgcctgcca cgagtacgcg gtcagcgtcg
      961 cgtccgcgcc gcccatgtcc tgcgcctcgt tgaccttgta gcgcccgcgc cggaggttga
     1021 cgagcttgta gaacgcggcc tcgccccacg tcccgaatcc gaaacagcgc tcgacgcagc
     1081 cgtggcggat cgcgatcacc ccgagctgca cgggcccgtc gaaaaggtgg atatcgcggc
     1141 tatacccacc caggcagatc atctgcaaaa gatcaggaat cgacagcaaa ctgagatcgc
     1201 cttccatgac tacctttgcg gtggcctcgc gcgcggaggc cgtgtccgac ctactcgcgg
     1261 gccgatgctc gttgcccggc cagcccgccg gcgcggcctc gcgacgaccc tccaagctcc
     1321 cgcacctccg agaccgttga tcccaatcgc atggcgccca acactcctgg acaagcgttc
     1381 cagagcccag acgaggcggc aaccgtgcgt ttaccctcca tgcctccgaa tgcggcacga
     1441 ctgcacatct gggccgcgtg gcaaactcgg tgacaaccgg gcagcggacg gcttcgtgat
     1501 gccgccgatc gcggcagtca ccgtcgctgc gagaaatgcg gagcggcgca tcgcggcgac
     1561 cgttccaccg gtgcgacggc ggtgtcgcgg tgggcccgcg gtcgcagcgc cgctgcc
//
LOCUS       DASVOR010000530         6549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413495,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000530 DASVOR010000000
VERSION     DASVOR010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413495"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VF516_10605"
     CDS             <1..681
                     /locus_tag="VF516_10605"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEX8108169.1"
                     /translation="DRRTQSAPAARALIEATAPRREQRREPRAYGCVGLLERDLVAGG
                     VGDPIEQVLDRVMITTELARTLTRYKAWANEITFSTVAALPEGEATKPRPTIFKNMVH
                     TLNHPYVIDCIFQAHLEGRPHGFTARNTPTHPPLDELWRAVKAMDRWYIELSDRLSDR
                     ELAETIRFQFVGGGDGAMTRGEIILHLVNHATYHRGFVGDMLNQAQVRPPATDLTVYL
                     RDIHRAAA"
     gene            complement(705..1598)
                     /locus_tag="VF516_10610"
     CDS             complement(705..1598)
                     /locus_tag="VF516_10610"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8108170.1"
                     /translation="MARRRRDYYAVLGVSRDASDVEIKRAFRELARRHHPDVGPADNG
                     EAFREINEAYAVLSDAETRARYDRWGHHDDPGGLSAVVEAAQDILNDVFRRRRGKQRG
                     RDLRYTLELTFEEAVFGCSKAIQIPTGEGRIRDFIVVVPAGTKDGGTKTFKSEGEPGK
                     GGAAAGDLHVHLRVAEHAVFRRDGLDVRSEHIIGFPQAALGAVLEITTLDGPIKMRIP
                     EGTQPGQVFRIRGRGIPQAAGKTAPRGDHLVTIQIAVPTELSPRQRQLIEELAQAGGE
                     LPGGEAPRKRLLDRVLSLLEE"
     gene            complement(1598..3430)
                     /gene="dnaK"
                     /locus_tag="VF516_10615"
     CDS             complement(1598..3430)
                     /gene="dnaK"
                     /locus_tag="VF516_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8108171.1"
                     /translation="MARVVGIDLGTTNSCVAVIDGDAPVVIANAEGTRTTPSVVGFAQ
                     TGERLVGQAARRQAMTNAENTIYAVKRLMGRKFEDSEVQRHVLTCPYEVVAADNGDAH
                     VRVRGRDFSPPEISAQVLQKMRQTAEDWLGEQITEAVVTVPAYFDDAQRQATKDAGRI
                     AGLNVLRIVNEPTAAAVAYGVETEGAQRVAVYDLGGGTFDVSILELQDGVFRVRSTAG
                     DTFLGGEDFDNAIVEHLLASFTATNDGMDLRGDRMALQRLKEAAERAKIELSSGERTE
                     INLPFIAAGPDGPRHLQLVFERHELEVLVEPLVQTTLEPCRRALADAGMTEADIDVVI
                     LVGGQTRMPRIQSLVADLFGKEPSRRVDPDEVVAVGAAIQGGVLTGEVQEVLLLDVTP
                     LSLGIEIQGGVFHRLIERNTALPARATEIFSTTIDNQPFVNVHVLQGERPMVADNKSL
                     ANFELTGLPPAPRGVPKIEVAFDIDADGVLAVSARDHGTGRVQRVTVTPTTGLSEADI
                     ERLVAESVEMADADVIRRQLVEARNKGEGLLYSSEKAMEEFGALLPDADREFLVVELA
                     ECRAAIAGDDLAAVQDAVARLEVSAQRIGEAIYAAAEPAPGGEA"
     gene            complement(3434..4120)
                     /gene="grpE"
                     /locus_tag="VF516_10620"
     CDS             complement(3434..4120)
                     /gene="grpE"
                     /locus_tag="VF516_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEX8108172.1"
                     /translation="MSGEPNQNAEPKDASAEAGNGADHGELPVEVVPPERAGRDGATG
                     AAGGDGQGAPITAPEAQVSEVSEKDELEARITALEKDKKDNWDRYLRTAADLENLRKR
                     QKREMDDVRLESKGRVLKEMLPVVDNLERAIEHATAQAGTNPIIEGVQLVLRQFLTAF
                     ERLDVTPVEAAGQPFDPNLHEAISQQESDAPPGTVVQVLQRGYKSGDRLLRPALVVVA
                     KAKSAPPPAE"
     gene            complement(4197..5225)
                     /gene="hrcA"
                     /locus_tag="VF516_10625"
     CDS             complement(4197..5225)
                     /gene="hrcA"
                     /locus_tag="VF516_10625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEX8108173.1"
                     /translation="MSSSELSRRAQKILHAVVTEYLHAGEAVGSRTVTRRHDLKLSPA
                     TVRNVMADLEELGLLEQRHSSAGRVPTPSGLRFFINELLKVRGLSAREKDEIRERVSA
                     PTRDEVVQRASRLLSDLTQHAAVIVTPDPVHQRLSHIEFVPLRDGKLITVLVTADGSI
                     ENRLIVDDVDPRRLEAIHNYLNQLLKGMTLDEVRERVIRELGEDKNRYDEAISAALRL
                     GHAVFVARERSADVVISGQANLIDDAIAQDNARELLRTLEDKETLIRLLDRTRHAEGL
                     QVFLGAETALSALAGSSVIAVSYGPEEQPIGALAVIGPMRMNYGKVMSVVDVTADTVS
                     QLLAELAT"
     gene            complement(5222..6340)
                     /locus_tag="VF516_10630"
     CDS             complement(5222..6340)
                     /locus_tag="VF516_10630"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEX8108174.1"
                     /translation="MLSLGVYLHFPWCRKRCPYCDFAVEVGAPPHDAYRDAIARELDL
                     RAGRFTGELVSIYLGGGTPSLWRPDCIAAAIEGIRQRFAAAGPLEVTLEANPVDCHAD
                     QLAAWRAAGVNRLSIGVQSLSPRELVVLGRDHRFGDGRAAVERALAAGFATSADFILG
                     VPGGPAEARVPDVLSADHLSVYELTIEERTAFGQRVRDGRLVPLPDDALAELYVATHD
                     RLTAAGYEHYEISSYARPGRRAVHNSLYWRGAAFLGLGVGAASLELDARGGGRRVTNP
                     RRAVDYLAAPGVPAEVTECAPAELAADRAWLGLRTSDGVTEAELAAAPGLADALVAER
                     LAERRAGRICPTLRGFLLADRIAARVVQSWSARLWESG"
     gene            6339..>6549
                     /locus_tag="VF516_10635"
     CDS             6339..>6549
                     /locus_tag="VF516_10635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108175.1"
                     /translation="MPPVYLDNAASTRIDDAVLALMTEVMRTAWGNPSSQHPQGAAAR
                     DHIATARRRLLAALGDPGGGGASPLG"
BASE COUNT          896 a         2406 c         2290 g          957 t
ORIGIN      
        1 gatcgtcgca cgcaatccgc gcccgctgct cgcgccctga tcgaggcgac cgcgccgcgg
       61 cgtgagcaac gccgcgagcc gcgagcctac ggctgcgtcg gccttcttga gcgcgacctc
      121 gtcgccgggg gcgttggcga tccgatcgaa caggttcttg atcgcgtcat gatcacgacc
      181 gagctggccc gcacgctgac ccgctacaag gcgtgggcga acgagatcac gttctcgacc
      241 gtcgctgcgc tccccgaggg cgaggcgacc aagccccgcc cgacgatatt caagaacatg
      301 gtccacacgc tcaatcatcc gtacgtcatc gactgcatct tccaggccca cctcgagggc
      361 cgcccgcacg ggttcaccgc gcgcaatacg ccgacccacc cgccgctcga cgagctatgg
      421 cgggcggtga aagccatgga tcgctggtac atcgagctga gcgaccggct gtccgaccgc
      481 gagctcgccg agacgatccg gttccagttc gtcggcggag gcgacggcgc gatgacgcgc
      541 ggcgagatca tcctccacct ggtcaaccac gcgacctacc accgcgggtt cgtcggcgac
      601 atgctcaacc aggcccaggt ccggccgccg gcgaccgacc tgacggtgta cctgcgcgac
      661 atccaccgcg cggcggcctg aatggggtga accgggttgc cgcgtcactc ctcgagcagc
      721 gacagcacgc gatcgagcag gcgcttgcgc ggcgcctcgc cgccgggcag ctcgccgccg
      781 gcctgggcga gctcctcgat cagctggcgc tggcgcggcg acagctcggt cggcaccgcg
      841 atctggatcg tgaccaggtg atcgccgcgc ggcgcggtct tgccggcggc ctgcgggatg
      901 ccgcggccgc ggatccggaa cacctggccc ggctgggtgc cctcggggat ccgcatcttg
      961 atcgggccgt cgagcgtggt gatctcgagg accgcgccga gcgcggcctg cgggaacccg
     1021 atgatgtgct cgctgcgaac gtcgaggccg tcgcggcgga acacggcgtg ctcggcgacc
     1081 cgcaggtgga cgtggagatc gccggcggcg gccccgccct tgccgggctc gccctcgctc
     1141 ttgaaggtct tggtgccgcc gtccttggtg cccgcgggga ccacgacgat gaagtcgcgg
     1201 atccggccct cgccggtcgg gatctggatc gccttgctgc agccgaacac ggcctcctcg
     1261 aaggtcagct cgagggtgta gcgcaggtcg cggccgcgct gcttgccgcg gcggcggcgg
     1321 aacacgtcgt tgaggatgtc ctgcgcggcc tcgaccacgg cggacagccc gcccggatcg
     1381 tcgtggtggc cccagcggtc gtagcgcgcg cgggtctcgg cgtcggacag caccgcgtag
     1441 gcctcgttga tctcgcggaa cgcctcgccg ttgtcggcgg gaccgacgtc ggggtggtgg
     1501 cgccgggcca gctcgcggaa cgcgcgcttg atctcgacgt cgctcgcgtc gcgcgacacg
     1561 ccgagcaccg catagtagtc acgccggcgc cgcgccatca ggcctcacca ccgggtgccg
     1621 gctcggcggc ggcgtagatc gcctcgccga tccgctgcgc agagacctcg agccgggcga
     1681 ccgcgtcctg gaccgcggcg agatcgtcgc ctgcgatcgc ggcgcggcac tcggcgagct
     1741 cgacgaccag gaactcgcga tcggcatcgg gcagaagtgc tccgaactcc tccatggctt
     1801 tctccgaaga gtagagcagg ccctcgccct tgttgcgagc ctccacgagc tgacgccgaa
     1861 tcacgtcggc gtccgccatc tcgaccgact cggcgaccag ccgctcgatg tcggcctcgg
     1921 acagcccggt ggtcggggtc acggtgacgc gctggacccg gccggtcccg tgatcccgcg
     1981 cagatacggc gagcacgccg tcggcgtcga tgtcgaacgc gacctcgatc ttgggcacgc
     2041 cgcgcggcgc gggcggcagg ccggtcagct cgaagttggc gagcgacttg ttgtccgcga
     2101 ccatcggccg ctcgccctgc agcacgtgga cgttgacgaa cggctggttg tcgatcgtcg
     2161 tcgagaagat ctcggtcgcg cgcgccggca gcgcggtgtt gcgctcgatc agcctgtgga
     2221 acacgccgcc ctggatctcg atgccgagcg acagcggcgt gacgtcgagc agcaggacct
     2281 cctggacctc gccggtcagc acgccgccct ggatcgcggc gccgaccgcg acgacctcgt
     2341 cgggatcgac ccggcggctc ggctccttgc cgaacaggtc ggcgaccagc gactggatcc
     2401 gcggcatccg ggtctggccg ccgaccagga tcacgacgtc gatgtcggcc tcggtcatcc
     2461 cggcgtcggc gagcgcgcgc cggcacggct cgagcgtggt ctggaccagc ggctcgacca
     2521 gcacctcgag ctcgtggcgc tcgaacacca gctggaggtg gcgcggcccg tcggggcccg
     2581 ccgcgatgaa cggcaggttg atctcggtgc gctcgccgct cgacagctcg atcttggccc
     2641 gctcggcggc ctccttgagc cgctgcagcg ccatccggtc gccgcgcagg tccatgccgt
     2701 cgttcgtcgc ggtgaacgac gcgagcaggt gctcgacgat cgcgttgtcg aagtcctcgc
     2761 cgccgaggaa cgtgtcgccg gcggtcgagc ggacccggaa cacgccgtcc tgcagctcaa
     2821 ggatcgagac gtcgaacgtg ccgccgccga ggtcgtagac cgcgacccgc tgggcgccct
     2881 cggtctcgac cccgtaggcc accgccgccg cggtcggctc gttgacgatc cgcagcacgt
     2941 tgagcccggc gatccggccg gcgtccttgg tcgcctggcg ctgggcgtcg tcgaagtacg
     3001 ccggcacggt gaccaccgcc tcggtgatct gctcgccgag ccagtcctcg gcggtctgcc
     3061 gcatcttctg gagcacctgc gccgagatct cgggcggcga gaagtcgcgg ccgcggaccc
     3121 ggacgtgggc atcgccgttg tcggccgcga cgacctcgta cgggcaggtc agcacgtggc
     3181 gctggacctc ggagtcctcg aacttgcggc ccatcaggcg cttgaccgcg tagatcgtgt
     3241 tctcggcgtt ggtcatggcc tggcgccgcg cggcctggcc gaccaggcgc tcgccggtct
     3301 gggcgaagcc gaccaccgac ggcgtggtcc gcgtgccctc ggcgttggcg atcaccaccg
     3361 gcgcgtcgcc gtcgatcacc gcgacgcacg agttggtcgt gccgagatcg atgccgacga
     3421 cgcgggccat gcgctactcg gcgggcggcg gggcggactt ggccttggcc accaccacca
     3481 gcgccggccg cagcagccga tcaccggact tgtacccgcg ctgcagcacc tgcacgacgg
     3541 tgccgggcgg cgcgtcggac tcctgctgcg agatcgcctc gtgcaggttg ggatcgaacg
     3601 gctggcccgc ggcctcgacc ggcgtgacgt cgagccgctc gaacgcggtc aggaactggc
     3661 gcagcacgag ctggacgccc tcgatgatcg ggttggtccc ggcctgggcc gtcgcgtgct
     3721 cgatcgcccg ctcgaggttg tcgaccaccg gcagcatctc cttgagcacc cggcccttgc
     3781 tctccagccg cacgtcgtcc atctcgcgct tctggcgctt gcgcaggttc tccaggtcgg
     3841 cggccgtccg caggtagcga tcccagttgt ccttcttgtc cttctccagc gctgtgatcc
     3901 gggcctcgag ctcgtccttt tcactgacct cgctgacctg ggcctccggg gcggtgatcg
     3961 gtgcgccctg gccatcgccg cccgccgcgc ccgtcgcacc gtcccggcca gcccgctccg
     4021 gcggcacgac ctcgaccggc agctccccgt ggtccgcccc gttcccggcc tcggccgagg
     4081 cgtccttggg ctccgcattc tggttgggtt ctccgctcat cgccacgacc ataaccacgt
     4141 ttgcgcggcc agaccagcgg cggccagcca gttgcaacgc gcgatcgcct ggccgatcag
     4201 gtggcgagct ccgccagcag ctgagacacg gtgtcggcgg tgacatccac gactgacatc
     4261 accttaccgt agttcatcct catgggcccg atcacggcga gcgcgccgat cggctgctcc
     4321 tcgggcccgt acgacacggc gatcaccgag ctgccggcga gcgcgctgag cgcggtctcg
     4381 gccccgagga acacctgcag cccctcggcg tgccgggtcc gatcgagcag ccggatcagc
     4441 gtctccttgt cctcgagcgt gcgcagcagc tcacgtgcat tgtcctgcgc gatcgcgtcg
     4501 tcgatcaggt tggcctgacc gctgatcacg acgtcggccg agcgctcgcg cgcgacgaac
     4561 acggcgtggc cgagccgcag cgcagcgctg atcgcctcgt cgtagcggtt cttgtcctca
     4621 ccgagctcgc ggatcacccg ctcgcggacc tcgtcgagcg tcatcccctt gagcagctgg
     4681 ttcaggtagt tgtggatcgc ctcgagccgg cgcggatcga cgtcgtcgac gatcagccgg
     4741 ttctcgatgc tgccgtcggc ggtgaccagc acggtgatca gcttgccgtc gcgcagcggc
     4801 acgaactcga tgtggctgag ccgctggtgc accggatccg gggtgacgat caccgcggcg
     4861 tgctgggtca gatcgctgag caggcgcgac gcgcgctgga cgacctcgtc gcgggtcggc
     4921 gccgataccc gctcgcggat ctcgtccttc tcgcgcgccg acaggccgcg gaccttgagc
     4981 agctcgttga tgaagaaccg caggcccgac ggggtcggca cccggcccgc cgacgagtgg
     5041 cgctgctcga gcaggccgag ctcctcgagg tcggccatga cgttgcgcac ggtcgccggc
     5101 gacagcttga gatcgtggcg gcgggtcacc gtccgcgagc cgaccgcctc ccccgcatgg
     5161 aggtactcgg tcaccaccgc gtgcaagatc ttctgcgctc gtcgcgatag ctcggagcta
     5221 ctcatccgct ctcccacagt ctagcactcc agctctgcac cacgcgggcc gcaattcgat
     5281 cggccaggag aaagccgcgc aaggtcgggc agatcctgcc agcccggcgc tcggcgagcc
     5341 gctcggcgac cagcgcgtcg gccagcccgg gagcggcggc gagctcggcc tcggtcaccc
     5401 cgtcggaggt ccgcaggccc agccaggcgc ggtcggccgc cagctcggcc ggggcacatt
     5461 cggtcacctc ggcgggcaca cccggagcgg ccaggtagtc cacggcccga cgcggattgg
     5521 tgacccgtcg ccccccaccg cgggcgtcga gctccagcga cgccgcgccc acccccaggc
     5581 cgaggaatgc ggcgccgcgc cagtacagcg agttatgaac cgccctccgg cccggccgcg
     5641 cgtacgagct gatctcgtag tgctcgtagc ccgcggcggt cagccggtcg tgggtggcga
     5701 cgtagagctc ggcgagcgcg tcgtcgggca gcggcaccag ccggccgtcg cgcacgcgct
     5761 ggccgaacgc cgtgcgctcc tcgatcgtca gctcgtacac cgagaggtgg tcggccgaca
     5821 gaacgtcggg cacgcgcgcc tccgccggcc ctcccggcac gccgaggatg aagtcggccg
     5881 aggtcgcgaa cccggcggcg agcgcgcgct cgaccgcggc gcggccgtcg ccgaaccggt
     5941 gatcgcggcc gagcacgacc agctcgcgcg gcgacagcga ctgcacgccg atcgagagcc
     6001 ggttgacgcc ggcggcgcgc cacgcggcga gctggtcggc gtggcagtcg accgggttgg
     6061 cttccagagt cacctcgagc ggcccggccg ccgcgaaccg ctgccggatc ccctcgatcg
     6121 ccgcggcgat gcagtccggg cgccacagcg acggcgtacc gccgccgagg tagatcgaca
     6181 ccagctcgcc ggtgaaccgg ccggcgcgca ggtcgagctc gcgcgcgatc gcgtcgcggt
     6241 aggcgtcgtg cggcggcgca ccgacctcga ccgcaaagtc gcagtacgga caccgcttgc
     6301 ggcaccatgg gaagtggagg tagacgccta gggacagtat gccaccagtc tatctcgaca
     6361 atgccgcgtc gacccggatc gacgacgcgg tgctcgccct gatgaccgag gtgatgcgga
     6421 ccgcgtgggg caacccgagc tcgcagcacc cccagggcgc cgccgcccgc gatcacatcg
     6481 ccaccgcgcg gcgccggctg ctggcggcgc tcggcgatcc gggcggcggc ggcgcgagcc
     6541 cgctcggcg
//
LOCUS       DASVOR010000531         3309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_5064,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000531 DASVOR010000000
VERSION     DASVOR010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5064"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3152
                     /locus_tag="VF516_10640"
     CDS             <1..3152
                     /locus_tag="VF516_10640"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8108176.1"
                     /translation="DQPMLFVYPINDIALAPRTGDGEYSHACYVGTETGVCFCLLVGF
                     VAAERERRNIVARQLWRDLHRGPVVAVRPWSDTHKDDLYDEPVVFVATQTGNLSIYHA
                     GHGSQTEPRLRGSGNYRFEGIRFDRIDLPAGLTSWAIRQGRSAFLAAYPNGRLLFGEL
                     YGPRESTVRDGLLSKLDELYGKVNLASAFFGNDERATDELEICAMIRIGDGALRSYIL
                     RKQLEQRAWDSLGEAEIDALLAGYLDRLSPEVVEERDQIKIVIDVVTSHVLDRSPAEI
                     LENCRPGRSYDKVARYGHVVRTCERLKRYLLDGAVQAQPGVIRVRMVIMAALFRAHVF
                     SYLPVDDASQRRIRKALREVLTACLRDDDRIVRIEALRAVAAALRSIRVLFDQADDDA
                     HREELRASFFPRGPKTIQWLVDALLENFARYRRHGEPVLLSTAWSYTTVLVAMIRLFP
                     GDALELCDQISRWGLGDNLEILAERLRGRHVESVRWRIRELCVVPSVRQQAQAQARDE
                     FIRAFEDVNVGQALAEHKIAPSDGDYLRASELLHAYRRLALLWKVNHQADIADIPLRW
                     SHSGELPEMSGSLAGVKRSLEALVEIARAGLDERAARLRELRSQITSGWRDGHKPPDA
                     VRLVLERVIERWQAVVEPKIPTPGEKIGLYVLGRLITVRGNGLVYELEGDSTRVMKVL
                     RDPLDMAARQEFNRDARLSRELAERYPASFVGVHSLEPTSGYAVMDRFKPVRELDPVK
                     RAPLAAIAAPQLARALRCLHEEDLAHGNLRAANVFVTEGPESAFRLGDLHAVTGSDAW
                     PQAAHQIPRLLQEPAATVKPAHWIDRVSLALFLAWLVTGETLAQDADAQALAQLASRL
                     KTCGVTGGDGIARMLLHLWERSPTTAHQLEWLIGPGMPSGFERKPLAGRPLVRFDVFI
                     SYNSQNIAVARSLYTELVGRGLVPYLDEREAVSDRWRLEIRHAMDECRAFAILIGSKY
                     RKDAAQPKEVSAAREESQKHNLPLKVFLLGSERPAEFQAVLQDHPVIQVEQNVRLMAD
                     EIVRSFQPPSARPGNDPAL"
BASE COUNT          491 a         1161 c         1174 g          483 t
ORIGIN      
        1 acgaccagcc gatgctgttc gtctacccga tcaacgacat cgcgctcgca ccgcggacgg
       61 gtgacggcga gtacagccac gcgtgttacg tcggcaccga gaccggtgtg tgcttctgcc
      121 tgctggtcgg cttcgtcgcc gcggagcgcg agcgccggaa tatcgtcgcc cggcagctgt
      181 ggcgagacct gcaccgcggg ccggtcgtcg cggtccggcc gtggtccgat acccacaagg
      241 acgacctgta cgacgagccg gtcgtgttcg tggcgaccca gaccgggaac ctgtcgattt
      301 accatgcagg ccacggctcg cagaccgagc cccggctccg cggtagcggg aactaccggt
      361 tcgagggcat acggttcgat cgcatcgacc tgccggcggg cctgacctcg tgggcgatcc
      421 ggcagggccg ctcggcgttc ctcgccgcct accccaacgg ccggctgctg ttcggcgagc
      481 tctacggtcc gcgcgagtcg acggtgcgcg atggcctgct gagcaagctc gacgagctct
      541 acggcaaggt gaacctcgcc agcgcgttct tcggcaacga cgagcgcgcc accgacgagc
      601 tcgagatctg cgcgatgatc cgcatcggcg acggtgcgct gcgtagctac atcctgcgca
      661 agcagctcga gcagcgggcg tgggactcgc tcggtgaggc ggagatcgac gccttgctcg
      721 ccggctacct cgaccggctc agtcccgagg tggtcgagga acgcgatcag atcaagatcg
      781 tcatcgacgt ggtgacgagc cacgtgctgg accgcagccc ggccgagatc ctggagaact
      841 gccggcccgg ccggtcctac gacaaggtcg cccgctacgg gcatgtggtc cggacctgcg
      901 agcgcctgaa gcgctacctg ctcgacggcg cggtgcaggc ccagcccggc gtgatccgcg
      961 tccgcatggt gatcatggcc gcgctgttcc gggctcatgt gttcagctac ctgccggtcg
     1021 acgatgctag ccagcgccgg atccgaaagg cgctgcgcga ggtgctcacc gcctgcctgc
     1081 gcgacgacga tcggatcgtg cggatcgagg cgctgcgcgc ggtggcggcc gcgctgcgca
     1141 gcatccgggt gctgttcgac caggccgacg acgacgccca tcgcgaggag ctccgcgcga
     1201 gcttcttccc gcgcgggccg aagacgatcc agtggctggt cgacgcgctg ctcgagaact
     1261 tcgcgcggta ccgccgccac ggcgagccgg tgctgctgtc gaccgcatgg tcctacacca
     1321 cggtgctggt ggcgatgatc cggctgtttc cgggcgatgc cctggagcta tgcgatcaga
     1381 tctcgcggtg ggggctcggc gacaacctcg agatcctcgc ggagcggctg cgcggccgcc
     1441 acgtcgagtc cgtgcgctgg cggatccgcg agctgtgcgt ggtgccgtcg gtgcgccagc
     1501 aggcgcaggc gcaggcccgc gacgagttca tccgggcgtt cgaggacgtc aacgtcgggc
     1561 aggccctggc cgagcacaag atcgccccga gcgacggcga ctacctccgg gccagcgagc
     1621 tgctgcatgc ctaccgccgc ctcgcgctgc tgtggaaggt gaaccaccag gcggacatcg
     1681 ccgacatccc gctgcgctgg agccactccg gcgagctgcc cgagatgtcc ggcagcctcg
     1741 ccggcgtcaa gcgcagcctg gaagcgctgg tcgagatcgc gcgggccggg ctcgacgagc
     1801 gcgccgcgcg gctccgcgag ctgcgatcgc agatcacgag cggctggcgc gacggccaca
     1861 agccgcccga cgccgtgcgg ctcgtgctcg agcgcgtcat cgagcgctgg caggcggtcg
     1921 tcgagccgaa gatcccgacc ccgggggaga agatcggcct ctacgtcctg ggtcgcctga
     1981 tcacggtgcg cggcaacggc ctggtctacg agctggaggg cgattcgacc cgcgtcatga
     2041 aggtcctgcg cgatccgctg gacatggccg cgcgccagga gttcaaccgc gatgcccgtc
     2101 tgagccgcga gctggccgag cgttatccgg cgtcgttcgt cggcgtccac tcgctcgaac
     2161 cgacgtccgg ctacgcggtc atggatcggt tcaagccggt ccgcgagctc gacccggtca
     2221 agcgagcccc gctggcggcg atcgccgctc cgcagctcgc acgagcgctg cgctgtcttc
     2281 acgaggagga cctggcacac ggcaacctgc gcgccgccaa cgtgttcgtc accgaagggc
     2341 ccgagtcggc gttccggctg ggcgacctgc acgcggtcac cggcagcgac gcatggccgc
     2401 aggccgcgca ccagatcccg cgcctcctgc aggagccggc ggccaccgtc aagccggctc
     2461 actggatcga tcgcgtgtca ctggccctgt tcctcgcctg gctggtcacc ggggaaaccc
     2521 tggcgcagga cgcggacgcc caggcgctcg ctcagctcgc gagcaggctc aagacctgcg
     2581 gcgtgaccgg gggcgacggc atcgcgcgca tgctcctgca cctgtgggaa cgatcgccga
     2641 ccaccgccca tcagctggag tggctgatcg ggccgggcat gccgtcgggg ttcgagcgca
     2701 agccgctggc cggcagaccg ctggtgcggt tcgatgtgtt catctcgtac aactcgcaga
     2761 acatcgcggt cgcgcgctcg ctctacaccg agctcgtggg ccgcggcctg gttccctacc
     2821 tcgacgagcg cgaggcggtg tccgaccgct ggcgcctcga gatccggcat gccatggacg
     2881 agtgccgtgc gttcgccatc ctgatcggga gcaagtaccg caaggacgcg gcccagccca
     2941 aggaggtgag cgccgcccgc gaggagagcc agaagcacaa cctcccgctg aaggtgttcc
     3001 tgctcggcag cgagcgcccg gccgagttcc aggccgtgct ccaggaccac ccggtgatcc
     3061 aggtcgagca gaacgtgcgg ctcatggcgg acgagatcgt ccggagcttc cagcctccga
     3121 gcgcccggcc gggcaacgac ccggcgctgt gatcggggca acgggccgca ccggcggcgt
     3181 ttgacccggc gctgccccgc ggggtccgat ggccgtgata gcctggacgg ccatgagggg
     3241 cgccatcgcc gcggggctgg tgatcgccgc gctggcgggg tgccacgccg aggccggccg
     3301 cgacacgct
//
LOCUS       DASVOR010000532         1708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_560412,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000532 DASVOR010000000
VERSION     DASVOR010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1708
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560412"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            256..>1708
                     /locus_tag="VF516_10645"
     CDS             256..>1708
                     /locus_tag="VF516_10645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108177.1"
                     /translation="MTPGKRLGNRFEIEQAIDSGGMGTVFRARDSLAGEVVAVKVISE
                     ERSHHTERFAREIKVLAELSHPGIVRYISHGTTPSGELFLVMEWLDGETVKSRLQRGP
                     LPASEAVTLAIRVAEALGAAHAHGIIHRDLKPSNLILPGGRIDQVKILDFGIAQRVGL
                     TRLTQTGAMIGTPGYMAPEQARAGGQIDARADVFALGCVLFQCLTGVPPFDGDTTAAI
                     LAKILFDAAPRVSELWPEVPPALDALVALALSKDPALRPSDGASLAAALTALGPVVST
                     APGAQRDRAASPRAATRRERRLLAVVMLGPPGQDDPLFEDPLRFAVAPHGGRLEQLAD
                     GSTVVILDADHQVATDQAVQAARCALAMSALARHRPLAIAMGRTESGSRLPEEDVIGR
                     ASRLLAHIARRPGDLPRIALDEVSAGLLDARFDVVDHDSQPRLRGERAVMHGARTLLG
                     RPTSCVGRDWELGALAGILDECIEEPAARVALVI"
BASE COUNT          235 a          655 c          589 g          229 t
ORIGIN      
        1 cctcatcttt gcaacacctg cgatgcgaga atctctcacc tgccccgacg tgcaacgtga
       61 gtgttccgtg aagacaacag agaaccgctc gacggcctgc gcctcggtcc cgtagggatg
      121 gacgacgcag tattccgtga aacacgacga ccggctcgag tccatccggg cacgctcatc
      181 acggcagggc cgccgccggc ccgcaggacg ccatcgctcc cggcgcccgc ggcggcacgc
      241 cacggagatc ccgtcatgac gcccgggaag cggctcggca accggttcga gatcgagcaa
      301 gcgatcgaca gcggcggcat gggaacggtg ttccgcgcgc gcgattcgct cgcgggtgag
      361 gtggtggcgg tcaaggtcat ctccgaagag cgcagccacc acaccgagcg cttcgcacgc
      421 gagatcaagg tgctcgccga gctctcgcac cccggtatcg tccgttacat ctcgcacggc
      481 acgacaccgt cgggcgagct gttcctcgtc atggaatggc tcgacggcga gaccgtcaag
      541 tcgcggctcc agcgcggccc gctgcccgcg agcgaggcgg tcaccctcgc gatccgcgtc
      601 gccgaggcgc tcggtgccgc gcacgcccac gggatcatcc accgcgatct caagccctcc
      661 aacctgatcc tccccggcgg tcgcatcgac caggtcaaga tcctcgactt cgggatcgcg
      721 caacgcgtgg ggctcacccg gctcacccag accggggcca tgatcggaac tcccggctac
      781 atggcccccg agcaggcccg cgccggcggc cagatcgacg cgcgcgccga cgtgttcgcg
      841 ctcggctgcg tgctgttcca gtgcctgacc ggcgtcccgc cgttcgacgg cgacaccacc
      901 gccgccatcc tggccaagat cctgttcgac gccgcgccgc gggtgagcga gctgtggccc
      961 gaggtgcccc cggccctgga cgcgctggtc gcgctggccc tgtcgaagga ccccgcgctg
     1021 cgtccgagcg acggcgccag cctggccgcg gcgctgaccg cgctcggtcc cgtggtcagc
     1081 accgcgcccg gcgcgcagcg ggaccgcgca gcgagccctc gcgcggcgac ccgcagggag
     1141 cggcggctcc tcgccgtggt gatgctgggg ccgcccggcc aggacgatcc gctgttcgag
     1201 gacccgctgc gcttcgcggt cgcgccgcac ggcggccgcc tcgagcagct cgccgacggt
     1261 tccacggtcg tgatcctcga cgccgatcat caggtcgcga ccgatcaagc cgtccaggcc
     1321 gcgcgctgtg cgctcgcgat gtcggcgctc gcccggcatc gcccgctcgc catcgcgatg
     1381 gggcgcaccg agtcggggag ccggctgccc gaggaagacg tgatcggtcg ggcgtcccgg
     1441 ctgctcgcgc acatcgcccg gcggccgggc gacctgccgc ggatcgcgct cgacgaggtc
     1501 agcgccggcc tgctcgacgc gcggttcgac gtcgtcgacc acgactccca gccccggctg
     1561 cgcggcgagc gcgcggtgat gcacggcgcg cggacgctgc tcggccggcc gacctcgtgc
     1621 gtcggccgcg actgggagct cggcgcgctc gccggcatcc tcgacgagtg catcgaggag
     1681 cccgccgcgc gcgtcgccct ggtgatcg
//
LOCUS       DASVOR010000533         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_93019,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000533 DASVOR010000000
VERSION     DASVOR010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93019"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            341..820
                     /gene="cynS"
                     /locus_tag="VF516_10650"
     CDS             341..820
                     /gene="cynS"
                     /locus_tag="VF516_10650"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:NF002773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEX8108178.1"
                     /translation="MKREQLTEKILDIKRDNGWTWKHITTQIGGVSPVLVVGALLGQM
                     KLVKPLARKAAALFGLSPSEERMLNEVPHRGSPMPPSDPLLYRLYELVMVNGPAWKAL
                     IEEEFGDGIMSAIDFDVEIERLPSPKGDRVKISMSGKFLPYKYYGNEAGIPDYGLKE"
BASE COUNT          279 a          512 c          521 g          261 t
ORIGIN      
        1 gcgacacgac ctgatccgac ttgccatcga acagatgagc ggccttgagg atggtgatct
       61 tcggcgccgc ggcgacgggg gcgaggccga gagcgaagac gaatgccagc ggagcgacgg
      121 cgagcgcgag cggagctctc atgccgcgga ccatacacgc gcgacagcga cgatgtctcg
      181 cctcacccga catctcgagg atcggaccgc ggccgcccgt gaagaccgcg gaccggtcgg
      241 cgcggtgggc cggactgttc cacggcaccg ggggccgggc cgccgggttc gccgcgctgc
      301 gatgcacagc gtcacgtacg ctgcggcgcg ggagatcgac atgaaacgcg aacagctcac
      361 cgaaaaaatt ctcgatatca agcgcgacaa cggctggacc tggaagcaca tcaccacgca
      421 gatcggcggc gtgtcgccgg tgctggtggt cggtgcgctg ctcggccaga tgaagctggt
      481 caagccgctc gcgcgcaagg ccgcggcgct gttcgggcta tcgccgagcg aggagcggat
      541 gctgaacgag gtgccgcacc gcggcagccc gatgccgccg agcgatccgc tgctctaccg
      601 gctgtacgag ctcgtgatgg tcaacggccc ggcctggaag gcgctgatcg aggaggagtt
      661 cggcgacggc atcatgtcgg cgatcgactt cgacgtcgag atcgagcgcc tgcccagccc
      721 caagggcgac cgcgtcaaga tctcgatgtc cggcaagttc ctaccttaca agtattacgg
      781 caacgaggcc ggcattcccg attacggctt gaaggaatga gcgccggcgc cgacgtcgga
      841 tttccgtgac cgcgtgatcg gacccgcgca tggtgccgta aaacgcggcc cgggatcttc
      901 cccatccgca acgcgcgatc cggcagagtg gccgctccac tcgcacggct gctccatatc
      961 tgtatccgcc acgctggaac gtcgcccgcc gccatgacca tgcttgcctc cgccggagtc
     1021 cgcgccttga acaggagcgc cgcgccgttc acgtgtcgcg tccgccggat ccgcgctgac
     1081 caggccaccg tcggggaacg ccggagcggc cgtccgggat ccggcggcgg gccggcgcgc
     1141 ggcgcggcga catgacgccc ggcgtgcggt gcgataccgc gctcggtcca tggcacatgt
     1201 ccgggtgggc tccggccggc gctcgccatg acggctgtgg tccaaccgcg atccggcggg
     1261 agtatccggc atgtcatgtt cttgtgatgc attgcacaaa tacctattgt gcaagctttg
     1321 tacgcagtcg agttttatat ggcagtctcc gacattgacg gtgacaatcg gacagtaaca
     1381 ttgtccggaa attaatatta gccttcattg acgagtggca tctcgatatt gtctagtgcg
     1441 gctcccgccg tacaacccat gtccagagga accgtgatga ccgcttccca gctgctgaag
     1501 atcctgtccc tgtcgtctgc cctggtggtc tctgcctgtg ccgccgatcc ggaggccacg
     1561 accgacgagc tga
//
LOCUS       DASVOR010000534         6501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413512,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000534 DASVOR010000000
VERSION     DASVOR010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6501
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413512"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VF516_10655"
     CDS             <1..606
                     /locus_tag="VF516_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX8108179.1"
                     /translation="LHAGRDAEARRFARAAIATLEQTGVDAIVVNAAGCGSSMKEWGH
                     LFADDPAWAERARALAAKVKDVSELLAGQPAVAERHPVRLRVAYHDACHLAHAQRIRT
                     EPRALLTAIPGLELIELDSDTCCGSAGVHNLLEPESAREIGERKVDSVLAAAPDLLVS
                     ANPGCTLQIQMLLRERGRDVRTAHPIEVLDASIRGVQPGRP"
     gene            792..2687
                     /locus_tag="VF516_10660"
     CDS             792..2687
                     /locus_tag="VF516_10660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEX8108180.1"
                     /translation="MYQQNYDPFGNVFLSTVVAAVPIVALLYFIALHPHRDRQGNRHL
                     GISAPYAALCGVIAAFLVSCVAFRMPVGAAVSAFVLGTLSGFVGIIWIVVAAMLLYTM
                     TVVTGKFEIVKESIIHISFDRRLQVLLIAFSFGAIIEGTSGFGTPVAIAGAIMVGLGF
                     KPFQSAVLNLIANTAPVAFGAIGTPIVTLAAVSQLDELQLSTMVGHQLPWASILVPFW
                     LVALFVKMEGGTWKQTFEVWPAVLVSGATFAIMQYLASSSLVLHNLTDVTAGGFSVIC
                     TAVFLRFVWHPKTRFLLRSEREAGAKAAIEATEWKYKYGLGETIHAWLPWAILIACCV
                     IWGLKKAWLNDVFAGVKFDTTLFGSKLTGTLSLPKWDMPGLQNLVQRMPPVAPPNAKP
                     EGAQFAFNWVSAVGTGVFVAAILSGFVLGLTAAQWKQAFRQTARRMVIPILVIGQVLG
                     LGFLTRYAGTDAVLGLMFANAGATYPFFAAYLGWLGVFLTGSDTASNALFGSLQKISA
                     QKLGISPILTCATNSSGGVMGKMIDAQSIMVACAATYEDPYERAHAIGPIFRKVWWHS
                     VAGAAFIGVIALIQSQMTGCIPEPPPPPAKSSRAEPAPRAPGPTGFDRDHRTELARAD
                     RPAAAPR"
     gene            2749..4425
                     /locus_tag="VF516_10665"
     CDS             2749..4425
                     /locus_tag="VF516_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108181.1"
                     /translation="MPSLRLCLVAVVAGALSCGGGGAPQPATLSSRWSAPSILAEVPA
                     DAPYLIALLEPMNDALRRRMMHNLDAQLAQSMKALDKLRADPGKLEPWMRAVLTLADE
                     LRAKGTAAWFDQLGIDPRGRFVIYGMSLWPVARIEIDHPDRLRATVARVMSAAGIQPA
                     PQRTLDGRAYWLSSSRDFSFVAAVLDREAVVAVLPTAVVDAALPQVLGTRPPDHSLAA
                     TTTVPDLLARHRFLGVLLGYFDAHRIIDVIAGPQPRPIDIPLRAATGPVSAECRADLD
                     RFAAAIPRLALGYHHFDDTGFDASMVLETPPATVAALRKLHTVVPEVTPRPAGHPLFA
                     MAAALDPDQLIAWLQDETRRIHDRPFRCPWFTEWNEAAADIAAKLATPLPPTWRGVRG
                     FSMTIDDASILPPTVTGHVLVAGDRVADLVQSLAGKVPAIAGIPLTATGRPIALPTRQ
                     LQIPVPAHFALTTDRLVLAAGEGSERRVTEHLRSPAPRASPLFTMAFDVPRLQQLLAS
                     LGQSPVDNLGSLGDVGLGLDIDDTGISFDVFGTWAPEASPNQIAAPPARP"
     gene            complement(4433..5389)
                     /locus_tag="VF516_10670"
     CDS             complement(4433..5389)
                     /locus_tag="VF516_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009239537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8108182.1"
                     /translation="MTTPAPSPRTRRWPVEYTVFAVMTALLVLAPLVVYPVTLMQCLC
                     LALFACAFNLLIGYVGLLSFGHAIFLGGAGYAAAHAAKVWGLTPELAILFGTACAAVA
                     GLVVGWLAIRRQGIYFSMITLGLAQMFYFFCKQADFTHGEDSIQAVPRGHLLGLINLD
                     NDLVMYGFVLLVFLIGFSIIYRIIHSPFGAVLTAIRENETRTTSLGYNPDHYKLIAFV
                     LSAALAGTAGATKALALQVASLTDVEWQTSGAVVLMTLIGGLGRYTGPVVGAFFVAIL
                     EHYLAKTRGLVPVLEGAVFVVFVLLFRQGVIGGLSNLLKLPL"
     gene            complement(5386..6264)
                     /locus_tag="VF516_10675"
     CDS             complement(5386..6264)
                     /locus_tag="VF516_10675"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8108183.1"
                     /translation="MGSITAQALYPQLLIGLINGASYALLSLGLSIIFGLLNVINFAH
                     GALYMLGAFVTWGLLHYAGVPYWGALILAPLAVGIVGVVLERTLLHRLYRLDPLYNLL
                     LTFGVTLLLQGLFQMQFGSSGEPYDIPAALQGAKDVGFMILPYYRGWVMIVALVVCLA
                     TWYVTERTRLGSYLRAATENPGLVQAFGINVPRMITVTYGLGVALAGFAGVLAAPTVS
                     VSPEMGSELIIVVFAVVVIGGMGSIMGSIVTGFALGLIEGLTKVLYPPASNTVVFALM
                     ALVLLIRPAGLFGKAS"
     gene            complement(6266..>6501)
                     /locus_tag="VF516_10680"
     CDS             complement(6266..>6501)
                     /locus_tag="VF516_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108184.1"
                     /translation="DGKAVVDKMKAMPTDDPLFGKGTIDPNGRKRHSMYVYEVKKPEE
                     SKAKWDYYKLVREVPADQAFRAPADSGCPLIQK"
BASE COUNT          974 a         2415 c         2152 g          960 t
ORIGIN      
        1 ctccacgccg gccgcgacgc cgaggcccgc cggttcgccc gtgcggcgat cgcgacgctg
       61 gagcagaccg gcgtcgacgc gatcgtcgtc aacgcggccg ggtgcgggtc gtcgatgaag
      121 gagtgggggc acctgttcgc ggacgatccg gcgtgggccg agcgcgcccg ggccctcgcc
      181 gccaaggtca aggacgtcag cgagctgctc gccggccagc ccgcggtcgc cgagcgccac
      241 ccggtccggc tccgggtcgc gtaccacgac gcgtgccacc tcgcgcacgc ccagcgcatc
      301 cgcaccgagc cgcgcgcgct gctcaccgcg atccccgggc tggagctgat cgagctcgac
      361 agcgacacct gctgcggcag cgccggcgtc cacaacctgc tggagcccga gagcgcccgc
      421 gagatcggcg agcgcaaggt cgacagcgtg ctcgccgccg cccctgatct cctggtcagt
      481 gccaacccgg gctgcacgct gcagatccag atgctgctgc gcgagcgcgg ccgggacgtc
      541 cgcaccgcgc acccgatcga ggtcctcgac gcctcgatcc gcggggtcca gcccggccgg
      601 ccctgaacgc ccgccctgaa cgcccgccct gaacgcccga cgaggtacgc ggccgggatc
      661 cctccatgac gcaccgtgca acgccacgga gttgccaatt cttatttcgt ttgtcggacc
      721 atctctctag attcgaggca gaccgccgcg tcaggcggtt cgatcggtaa cccgttcgag
      781 gagcgcctca cgtgtaccag cagaattacg acccgttcgg gaacgtgttc ctgtccaccg
      841 tggtggcagc agtgccgatc gtggcgctgc tgtacttcat cgccctgcat ccccaccgcg
      901 accgccaggg caatcgccac ctcggcatct cggcgcccta cgcggcgctg tgcggggtga
      961 tcgccgcgtt cctcgtgtca tgcgtggcgt tccgcatgcc ggtcggcgcg gcggtgtccg
     1021 cgttcgtgct cggcacgctg tccggcttcg tcgggatcat ctggatcgtc gtggccgcga
     1081 tgctgctgta cacgatgacc gtcgtcaccg ggaagttcga gatcgtcaag gagtcgatca
     1141 tccacatctc gttcgatcgc cggctccagg tcctgctgat cgcgttctcg ttcggcgcga
     1201 tcatcgaggg tacgtcgggc ttcggcacgc cggtcgcgat cgccggcgcg atcatggtcg
     1261 gcctcggatt caagccgttc cagtccgcgg tgctgaacct gatcgccaac accgcgccgg
     1321 tcgcgtttgg cgcgatcgga acgccgatcg tcacgctcgc cgcggtcagt cagctcgacg
     1381 agctccagct gtcgaccatg gtcggccacc agctgccgtg ggcgtcgatc ctcgtgccgt
     1441 tctggctggt cgcgctgttc gtcaagatgg agggtggcac ctggaagcaa acattcgagg
     1501 tctggccggc ggtactggtg tcgggagcga cgttcgcgat catgcaatac ctggcgtcga
     1561 gcagcctcgt cttgcacaac ctcaccgacg tcacggcggg cgggttctcg gtgatctgca
     1621 ccgcggtgtt cctgcgcttc gtgtggcacc ccaagacccg gttcctgctg cgctccgagc
     1681 gcgaggccgg cgcgaaggca gccatcgagg ccaccgagtg gaagtacaag tacgggctcg
     1741 gcgagaccat ccacgcctgg ctgccgtggg caatcctgat cgcctgctgc gtgatctggg
     1801 gcctgaagaa ggcctggctc aacgacgtct ttgccggcgt caagttcgac accacgctgt
     1861 tcggctccaa gctgaccggc acgctgtcgt tgcccaagtg ggacatgccg gggctgcaaa
     1921 acctggtgca gcgcatgccg ccggtcgcgc cgcccaacgc caagcccgag ggcgcgcagt
     1981 tcgcgttcaa ctgggtctcg gcggtgggga ccggcgtgtt cgtcgctgcg atcctctccg
     2041 gcttcgtgct cgggctgacc gccgcgcagt ggaagcaggc gttccgccag accgcgcgcc
     2101 gcatggtcat cccgatcctc gtgatcggcc aggtgctcgg cctcggcttc ctgacccgct
     2161 acgccggcac cgacgccgtg ctcggcctga tgttcgccaa cgccggcgcc acctacccgt
     2221 tcttcgcggc ctacctcggc tggctcggcg tgttcctgac cggctcggac accgcgtcca
     2281 acgcgctgtt cggcagcctg cagaagatca gcgcgcagaa gctcggcatc agtccgatcc
     2341 tgacctgcgc gaccaactcc agcggcggcg tgatgggcaa gatgatcgac gcgcagtcga
     2401 tcatggtcgc gtgcgccgcc acctacgagg acccctacga gcgcgcccac gcgatcggcc
     2461 cgatcttccg caaggtgtgg tggcactcgg tcgccggcgc ggcgttcatc ggcgtcatcg
     2521 cgctgatcca gtcgcagatg accgggtgca tccccgagcc gccgccgcca ccggccaagt
     2581 cgagccgcgc cgagcccgcc ccgcgcgccc ccggaccgac cggcttcgat cgcgatcacc
     2641 gcaccgagct cgcccgcgcc gaccgcccgg ctgccgcgcc tcggtaaccc gggcacgcgg
     2701 gtgcgcccac cgccgccgcg cgcggcggtt ctcggctaga gtccgcggat gccgtccctc
     2761 cggttgtgtc tcgtcgcggt cgtcgccggc gccctgtcgt gcggcggtgg cggcgctccc
     2821 cagcccgcca cgctgtcgtc gcgctggtcg gctccctcga tcctggccga ggtcccggcc
     2881 gacgcgccgt acctgatcgc gctgctcgag ccgatgaacg acgcgctgcg ccggcggatg
     2941 atgcacaacc tcgacgccca gctcgcgcag tcgatgaagg cgctcgacaa gctgcgcgcc
     3001 gaccccggca agctcgagcc gtggatgcgc gccgtgctca cgctcgccga cgagctgcgc
     3061 gccaagggca cggccgcctg gttcgatcag ctcggcatcg acccgcgcgg ccggttcgtc
     3121 atctacggca tgtcgctgtg gccggtcgcg cgcatcgaga tcgaccaccc ggacaggctg
     3181 cgcgcgacgg tcgcgcgcgt catgtcggcc gccggcatcc agcccgctcc gcagcgcacg
     3241 ctcgacggcc gcgcctactg gctgtcgagc agccgcgact tcagcttcgt cgccgcggtg
     3301 ctcgaccgcg aggccgtcgt ggcggtgctg ccgaccgccg tcgtcgacgc cgccctgccc
     3361 caggtcctcg gcacccgccc gccggaccac agcctggccg cgaccaccac cgtgcccgac
     3421 ctgctcgccc gccatcgctt cctcggcgtc ctcctcggct acttcgacgc ccaccgcatc
     3481 atcgacgtca tcgccggccc ccagccccgc ccgatcgaca tcccgcttcg ggccgccacc
     3541 ggcccggtgt ccgccgagtg tcgcgccgac ctcgaccgct tcgccgccgc gatcccgcgg
     3601 ctcgcgctcg gctaccacca cttcgacgac accgggttcg acgcctcgat ggtgctcgag
     3661 accccgcccg ccaccgtcgc cgcgctgcgc aagctccaca ccgtcgtccc cgaggtcacc
     3721 ccgcggcccg ccggccaccc gctgttcgcg atggccgccg cgctcgatcc cgaccagctg
     3781 atcgcctggc tccaggacga gacccgccgg atccacgatc gcccgttccg ctgcccgtgg
     3841 ttcaccgagt ggaacgaggc cgccgcggac atcgcggcca agctcgccac cccgctgccg
     3901 ccgacctggc gcggcgtgcg cggcttctcg atgacgatcg acgacgcctc gatcctcccc
     3961 ccgaccgtca ccggccacgt cctcgtcgcc ggcgaccggg tcgccgatct ggtccagtcg
     4021 ctcgccggca aggtccccgc gatcgccggc atcccgctca ccgcgaccgg ccggccgatc
     4081 gcgctgccga cccgccagct ccagatcccg gtgccagcgc acttcgcgct caccaccgac
     4141 cgcctggtcc tcgccgccgg cgaaggcagc gagcgccgcg tcaccgagca cctccgctcc
     4201 ccggccccca gggcctcgcc gctgttcacc atggccttcg acgtcccccg cctccagcag
     4261 ctcctcgcct cgctcggcca gtcgccggtc gacaacctcg gctcgctggg cgacgtcggc
     4321 ctcggcctcg acatcgacga caccggcatc agcttcgacg tgttcggcac ctgggccccg
     4381 gaggcctcgc cgaaccagat cgccgccccg cccgccaggc cgtgagcgct acctacagcg
     4441 gcagcttgag caggttgctc aggccgccga tcacgccctg gcggaacagc agcacgaaca
     4501 cgacgaacac ggcgccctcg aggaccggga ccaggccgcg ggtcttggcg aggtagtgct
     4561 cgaggatcgc cacgaagaac gcgccgacca ccgggccggt gtagcggccc aggccaccga
     4621 tcagcgtcat cagcacgact gcgcccgagg tctgccattc gacgtcggtc aacgaggcga
     4681 cctggagcgc gagcgccttg gtcgcgccgg cggtgccggc gagcgcggcc gacaggacga
     4741 acgcgatcag cttgtagtga tcggggttgt agccgagcga cgtggtgcgg gtctcgttct
     4801 cgcggatcgc ggtcagcacc gcgccgaacg gcgagtggat gatgcgatag atgatcgaga
     4861 atccgatcag gaacacgagc agcacgaagc cgtacatcac gaggtcgttg tcgaggttga
     4921 tcagcccgag caggtgaccg cgcggcacgg cctggatgct gtcctcgccg tgggtgaagt
     4981 cggcctgctt gcagaagaag tagaacatct gcgccaggcc gagggtgatc atcgagaagt
     5041 agatgccctg gcggcggatc gccagccagc cgacgaccag gccggcgacg gcagcgcacg
     5101 cggtgccgaa caggatcgcg agctcggggg tcaggcccca gaccttggcg gcgtgcgcgg
     5161 cggcgtagcc ggccccgccg aggaatatcg cgtggccgaa cgacagcagg ccgacgtagc
     5221 cgatcagcag gttgaacgcg cacgcgaaca gcgccaggca caggcactgc atcagcgtca
     5281 cgggatacac gacgagcggt gcgagcacga gcagcgcggt catcaccgcg aacaccgtgt
     5341 actcgaccgg ccaccggcgc gtgcgggggg atggggcggg ggtggtcacg acgcctttcc
     5401 aaacagcccg gccgggcgga tcaggaggac gagggccatc agcgcgaaca ccacggtgtt
     5461 cgacgccggg gggtagagga ccttggtcag gccctcgatc aggccgagcg cgaagccggt
     5521 cacgatcgag cccatgatcg agcccatgcc gccgatcacg acgacggcga acaccacgat
     5581 gatcagctcg ctccccatct ccggactgac gctgacggtc ggcgccgcga gcacgccggc
     5641 gaacccggcg agcgcgacgc cgaggccgta ggtcacggtg atcatgcgcg gcacgttgat
     5701 gccgaacgcc tggaccaggc cggggttctc ggtcgccgct cgcaggtacg agcccagccg
     5761 ggtgcgctcg gtgacgtacc aggtcgccag gcacaccacg agcgccacga tcatcaccca
     5821 gccgcgatag tagggcagga tcatgaagcc gacgtccttg gcgccctgga gcgccgccgg
     5881 gatgtcgtag ggctcgcccg aggagccgaa ctgcatctgg aacaggccct ggaggagcaa
     5941 cgtcacgccg aacgtgagca gcaggttgta cagcggatcg agccggtaca ggcgatggag
     6001 cagcgtgcgc tccaggacca cgccgacgat gccgaccgcg agcggcgcga ggatcagcgc
     6061 gccccagtac gggacaccgg cgtagtgcag gaggccccag gtcacgaacg cgcccagcat
     6121 gtacagcgcg ccgtgcgcga agttgatgac gttgagcagc ccgaagatga tcgacaggcc
     6181 caggctgagc agcgcgtagg acgcgccgtt gatcagcccg atcaacagct gcggatacag
     6241 cgcctgtgcg gtgatcgacc ccatgctact tctggatcag cgggcagccg ctgtcggccg
     6301 gggcgcggaa cgcctggtcg gccgggacct cgcggaccag cttgtagtag tcccacttgg
     6361 ccttcgactc ctcgggcttc ttgacctcgt agacgtacat cgagtgccgc ttgcggccgt
     6421 tgggatcgat cgtgcccttg ccgaacagcg gatcatcggt cggcatcgcc ttcatcttgt
     6481 cgaccaccgc cttgccgtcc t
//
LOCUS       DASVOR010000535         4498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_326429,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000535 DASVOR010000000
VERSION     DASVOR010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326429"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VF516_10685"
     CDS             <1..933
                     /locus_tag="VF516_10685"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8108185.1"
                     /translation="LASPLSALDLFDPGGYDARPALGERLPPAGILSSRGCPAACTFC
                     SNDVTGRRFRYRSAASVAAEIAVLRDRFGLVGFSFFDDSFAVGRRRVHELCAAIRGSG
                     GPVWWTCTAHPAHLDRDVLAEMRRGGCAGVDIGMESADPGMLVRIGKGVTVERVLAVL
                     GWCRDEGIHSVVNLMFGWPDETDAELDATIAFLDRAAPLAGGFNARGVVVPYPGTEIY
                     ERHHERFGFTGWWLREPPLAYAAFPTSWDEAEIRRAYADDPALDRNFFDHPPHRIARI
                     REALARKAASTVAIQYRRAAAATAPAGVPAAGAR"
     gene            1124..1936
                     /locus_tag="VF516_10690"
     CDS             1124..1936
                     /locus_tag="VF516_10690"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8108186.1"
                     /translation="MFLKYFEFIFRPFQKVNNQILGVKNIKGNLKVDINRVKSLKGRG
                     QDAMQRAQGYGQQFNQMGQQQGGQMPQQGQPPGMPGMPQMQGMPQMPGMPGMPGMGAP
                     QGPNPNPPVITKGFWIFAKKFCSQCEQQLDRSWDQCPYCAQIAQQVIAAPAKLEKAKT
                     QAFVIDAQGGPGSMQLLGWIVPLQGAQRGELFTLSPVTTVGTDPKCTIVLNDKFMSSK
                     HAEIKAENGVWVLRDSGSTNGTYVNNRRVDRHELVDNDFIKFGSAMLKFKSL"
     gene            1933..3447
                     /locus_tag="VF516_10695"
     CDS             1933..3447
                     /locus_tag="VF516_10695"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8108187.1"
                     /translation="MIKHNRRSLVVMGLVAACLAVPGRVHADASNIEVRMEVKPPDHN
                     NPKTKDDAPQIEVTVVGGPNSPADKFSLREDGAKQPVELKPTSKREYTQGTEKLAIAI
                     VMNGWEIWIGNDDVLPDGDPSRFPGVLKSLEAALDKVSFKDAGPPGSLGTVITYADSA
                     TIRIPMGSLDRITGSALGTQKDYFGTQGVELVKGVDLALSELHKVQVSRKVLIVICDG
                     LDKNMDAAKGQLIALKARATADKIETFAIIYKGGMSGDGNVISAMITQTQTVNTAENI
                     ATAIQSILSRMADRQYLTFPGFDPKLGLGLTWDGKPHNLVLKIDKDDADPVELTLAPP
                     WHLPKGGFPWLVVILVVLGALVLAVIGVKVFSSKPAPMPVPIVAPAPMEAPKPAGPMK
                     TVMMSAGGDDGGFPIVGWLVPLNGVQAYQTFKLRSGGTKIGTAQPCDIVINDGFMSTE
                     HCQINASPQGFTLVDGGSTNGSYVNERKIQGKQDLVDNDMVTLGKTNFKFKSIN"
     gene            3464..3925
                     /locus_tag="VF516_10700"
     CDS             3464..3925
                     /locus_tag="VF516_10700"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8108188.1"
                     /translation="MSTGVLIVLIIVVALILLGALVIVARANSRPQHQKICGGCRRVM
                     LPVWTKCLFCGWSPVARLEFILGPMANQTLSLVEEVTTIGSVAGNTVVLADPAVSRKH
                     AGIRKVNGLYELADLGSTNGVYVNGHKVPKKTLEPGDIIRVGNTEAVFKPE"
     gene            3996..>4498
                     /locus_tag="VF516_10705"
     CDS             3996..>4498
                     /locus_tag="VF516_10705"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8108189.1"
                     /translation="MARLLFRDSQGREGTVELSPTETVYVGRGLECAIRTDDGMVSRR
                     HSQIRMEAGRYVVEDLGSANGTHLNNNRIQKQALGHADIIQCGSLMIRFLDEGAVNIL
                     QQPIQGGGAPPPKKGGTMVIDRGEPPPPLAGGYGQPQPQPSAAGFGAQSAVNPYGGTA
                     YGGAPAMP"
BASE COUNT          775 a         1539 c         1560 g          624 t
ORIGIN      
        1 ctcgcctcgc cgctgtccgc gctcgacctg ttcgatcccg gcgggtacga cgcgcggccg
       61 gcgctgggcg agcggctacc gccggccggg atcctgtcga gccgcggctg cccggcggcc
      121 tgcacgttct gctcgaacga cgtcaccggg cggcggttcc gctaccgcag cgcggcgtcg
      181 gtcgccgccg agatcgccgt gctgcgcgac cggttcggcc tggtcgggtt cagcttcttc
      241 gacgactcgt tcgcggtcgg ccggcgccgg gtccacgagc tgtgcgcggc gatccgcgga
      301 tcgggcgggc cggtgtggtg gacgtgcacc gcgcaccccg cacacctcga tcgcgacgtg
      361 ctcgccgaga tgcggcgcgg cgggtgcgcg ggcgtcgaca tcggcatgga gagcgccgac
      421 cccggcatgc tggtgcggat cggcaagggc gtcacggtcg agcgcgtgct cgccgtgctc
      481 gggtggtgcc gcgacgaggg catccacagc gtggtcaacc tgatgttcgg ctggccggac
      541 gagaccgacg ccgagctcga cgcgacgatc gcgttcctcg accgcgcggc gccgctcgcc
      601 ggcgggttca acgcgcgcgg tgtggtcgtt ccctacccgg gcaccgagat ctacgagcgc
      661 caccacgagc ggttcggctt caccggatgg tggctccgcg agccgccgct ggcctacgcg
      721 gcattcccga ccagctggga cgaggccgag atccgccgcg cgtacgccga cgaccccgcg
      781 ctcgaccgca acttcttcga ccacccgccg caccggatcg cgcggatccg cgaggcgctc
      841 gcccgcaagg cggcgtcgac cgtcgcgatc cagtaccgtc gcgcggccgc cgcaacggct
      901 ccggcgggcg tcccggccgc cggagcgcgc taggcgatct cgccgaagcg tgccctgcca
      961 cagcggatct cggcgaccgg gtcggcggag gcggcaagga cggccggcgg gagtcccgaa
     1021 acgctttccg tggccggaag cgcacttcgc ccggccgtgg cgatgtgcga tgatgctaga
     1081 ctagccgcgc gcggagggat gccagcgcgt cccagaggga accatgttcc tcaagtactt
     1141 cgagttcatc ttccggccgt tccagaaggt caataaccag atcctcgggg tcaagaacat
     1201 caagggcaac ctcaaggtcg acatcaaccg cgtgaagtcg ctgaagggtc ggggccagga
     1261 cgcgatgcaa cgggcgcagg gatacggaca gcagttcaac cagatgggcc agcagcaggg
     1321 aggccagatg ccgcagcagg gacaaccacc ggggatgccg ggcatgcccc agatgcaagg
     1381 gatgccccag atgccgggca tgcccgggat gccaggaatg ggtgcgccgc aggggcccaa
     1441 tccgaacccg ccggtcatca cgaaggggtt ctggatcttc gccaagaagt tctgctcgca
     1501 gtgcgagcag cagctcgaca ggagctggga ccagtgcccg tactgcgcgc agatcgccca
     1561 gcaggtcatc gcggcgccgg cgaagctgga gaaggccaag acccaggcgt tcgtgatcga
     1621 tgctcagggc ggcccgggca gcatgcagct gctgggctgg atcgtcccgc tgcagggcgc
     1681 ccagcgcggc gagctgttca cgctgtcgcc ggtgaccacc gtcggcaccg acccgaagtg
     1741 cacgatcgtg ctgaacgaca agttcatgtc gtccaagcac gccgagatca aggccgagaa
     1801 tggcgtgtgg gtgctgcgcg actccggctc gaccaatggc acctacgtca acaaccgtcg
     1861 cgtcgatcgc cacgagctgg tggacaacga cttcatcaag ttcggcagcg cgatgctgaa
     1921 gttcaagagc ctatgatcaa gcacaaccga cgatcgctgg tggtgatggg gctggtggcg
     1981 gcctgcctgg cggtaccggg gcgggtccac gccgatgcgt cgaacatcga ggtccggatg
     2041 gaggtcaagc cgccggacca caacaacccc aagaccaagg acgacgcgcc gcagatcgag
     2101 gtgaccgtgg tcggcgggcc caactcgccg gccgacaagt tctcgctgcg cgaggacggc
     2161 gccaagcagc cggtcgagct caagccgacg tccaagcgcg agtacaccca gggcaccgag
     2221 aagctcgcga tcgcgatcgt gatgaacggc tgggagatct ggatcggcaa cgacgacgtg
     2281 ctgcccgacg gcgatccgtc gcggttcccc ggcgtgctca agagcctgga ggcggcgctc
     2341 gacaaggtct cgttcaagga cgccggcccg cccggcagcc tcggcacggt gatcacctac
     2401 gccgacagcg ccacgatccg gatcccgatg ggctcgctcg accggatcac cggcagcgcg
     2461 ctcggcaccc agaaggacta cttcggcacc cagggcgtcg agctggtcaa gggcgtcgac
     2521 ctcgcgctgt ccgagctcca caaggtccag gtgtcgcgca aggtgctgat cgtgatctgc
     2581 gacggcctcg acaagaacat ggacgcggcc aagggccagc tgatcgcgct caaggcgcgc
     2641 gcgaccgccg acaagatcga gaccttcgcc atcatctaca agggcggcat gtcgggcgac
     2701 ggcaacgtga tcagcgcgat gatcacgcag acccagaccg tgaacaccgc cgagaacatc
     2761 gccaccgcga tccagagcat cctgtcgcgc atggcggacc gccagtacct gacgttcccg
     2821 gggttcgacc ccaagctcgg gctcggcctg acgtgggacg gcaagccgca caacctggtg
     2881 ctcaagatcg acaaggacga cgccgacccg gtcgagctga cgctggcgcc gccctggcac
     2941 ctgcccaagg gcgggttccc gtggctggtc gtgatcctgg tcgtgctcgg cgcgctcgtg
     3001 ctcgccgtga tcggcgtcaa ggtgttcagc agcaagcccg cgccgatgcc ggtgccgatc
     3061 gtcgcgccgg cgccgatgga ggcgcccaag ccggcgggac cgatgaagac ggtgatgatg
     3121 agcgcgggcg gcgacgacgg cggcttcccg atcgtcggct ggctcgtgcc gctcaacggc
     3181 gtccaggcct accagacgtt caagctgcgc tcgggcggca ccaagatcgg caccgcgcag
     3241 ccgtgcgaca tcgtcatcaa cgacggcttc atgagcaccg agcactgcca gatcaacgcc
     3301 tcgccccagg gcttcaccct ggtcgacggc ggctcgacca acggcagcta cgtcaacgag
     3361 cgcaagatcc agggcaagca agatctggta gacaacgaca tggtcacgct cggcaagacc
     3421 aacttcaagt tcaagtcgat caactgagaa cagccaggtc gtcgtgtcca ccggtgtcct
     3481 gatcgtcctc atcatcgtcg tggcgctgat cctgctcggc gccctggtga tcgttgccag
     3541 ggccaactcc cggcctcagc accagaagat ctgcggcggc tgccgccgcg tgatgctgcc
     3601 ggtgtggacc aagtgcctgt tctgcggctg gtcgccggtg gcgcggctgg agttcatcct
     3661 cgggccgatg gccaaccaga cgctgtcgct ggtcgaggag gtcaccacga tcggctcggt
     3721 cgccggcaac accgtcgtgc tcgccgatcc ggcggtgtcg cgcaagcacg ccgggatccg
     3781 caaggtcaac gggctgtacg agctggccga cctcggctcg accaacgggg tctacgtcaa
     3841 cggccacaag gtgccgaaga agaccctcga gccgggcgac atcatccggg tcgggaacac
     3901 cgaagcggtg ttcaagcccg agtagggcgc gaggctccgg gtctggcgcc cggcctggcg
     3961 tccggccctc ggccccggta ccatcccagg acccaatggc acgactcctg ttccgggata
     4021 gccagggccg agaggggacg gtcgagctgt cgcccaccga gaccgtctac gtcggccgcg
     4081 gcctggagtg cgcgattcgc acggatgacg ggatggtgtc gcggcgccat tcgcagatcc
     4141 ggatggaggc cgggcggtac gtggtcgagg acctcggcag cgcgaacggg acccacctca
     4201 acaacaaccg gatccagaag caggcgctcg gccacgccga catcatccag tgcggctcgc
     4261 tgatgatccg gttcctcgac gagggcgcgg tcaacatcct gcagcagccg atccagggag
     4321 gtggcgcgcc gccgcccaag aagggcggga cgatggtgat cgaccgcggc gagccacccc
     4381 cgccgctggc gggcggctac ggccagcccc aacctcagcc ttcggccgcg gggttcggcg
     4441 cgcagtcggc cgtcaacccg tacggcggca ccgcgtacgg cggcgcgccg gcgatgcc
//
LOCUS       DASVOR010000536         3846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_93020,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000536 DASVOR010000000
VERSION     DASVOR010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3846
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93020"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /gene="sbcD"
                     /locus_tag="VF516_10710"
     CDS             complement(<1..868)
                     /gene="sbcD"
                     /locus_tag="VF516_10710"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HEX8108190.1"
                     /translation="MRLLHTSDWHLGHTLRGEVTREYEHAAFLAWLLETCVREAADAL
                     VITGDVFDTATPPASAERMWFEFLAAVRRARPAMDVVAIAGNHDSPARLGASSSVLRE
                     LGVHVIGGLPRCADGGLDLDRILIPVAGGRGLVAAVPFLRPVDVPGDAADPLGDVYGE
                     VIAAARARRRPDQALIVTGHLYVAGADTQYLSERRVSIGGQESAPLRLFPDDIDYVAL
                     GHIHRAQRVGRDAIRYAGAPLPLSLDEADYKSQIVAVELAGGRVCDLRPLAVPRVIDI
                     VRVPRRGAAPLAE"
     gene            1057..1569
                     /locus_tag="VF516_10715"
     CDS             1057..1569
                     /locus_tag="VF516_10715"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8108191.1"
                     /translation="MIVHTDRLVLRPVHRDDLEVLWAIHGDPETNRYNPAGPLPSRTA
                     AEELLRQWQSHWQDHDFGYWAIATREQPETVIGFGGVRLRHFRGAERLNLFFRLATST
                     WGKGYATEMGRAAVALAFDRLGHDAVFATTRPDNQPSIAVLQRLGFQRDGELPDDRGM
                     PSNVYVRHRS"
     gene            complement(1696..3627)
                     /locus_tag="VF516_10720"
     CDS             complement(1696..3627)
                     /locus_tag="VF516_10720"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8108192.1"
                     /translation="MSSSERGASDIASIIARIHRPKRAVVTAGMPYANGPVHLGHLAG
                     AQVPGDIHARWLGMVIGRPNVLYVCGTDEHGSTSELAALRDGVPIRELLDRVHDQQAT
                     TLKRYSIGLDVYTGTARPECFPLHKAMSQDFLRKLYRHGMLEKRSSRQWYDPKLERFL
                     PDRYVRGKCPNPKCDNESAYSDECDKCGHQHDPSELINPKSALSDATPVMRDTVHWFL
                     DMAKVAETLRVWLESKTGSWRPWVLSEVLGRVLPALRVAGDREAAYKELRASLPAHKM
                     KYTAGKNIVLQFKNRPDADAGAAALAAAGIATVPDDDWAHRSITRDVSWGIPLPDDLD
                     PEMAGKTLYVWPDSLIAPISFSQVALAARGEPAQRYAEFWRDPDARVYQFLGQDNVFF
                     YVLMQGAMWLGTQDDPHRLPAPGDLQLTDIFGCFHLLVSGEKMSKSRGNFITGDQLLD
                     QGYSADQIRYYLALLGLSEKPSNFDFDKLAERNAFLAGPMNATFERPISAAHSKFGGR
                     VPQGVLADKIVTDTQRIVQRYVKGMERADYPTMLYEVENYARIINSLFNQYKPHDDRR
                     PEEGRRDALYASFYVLKNLMIMLYPFAPDTMDRVRDSLRLPPEVFRVEELGVPLPAGH
                     EVGPKQPYFPGEAERKTRE"
BASE COUNT          534 a         1429 c         1327 g          556 t
ORIGIN      
        1 cctcggccag cggcgccgcc ccgcgccgcg gcacccgcac gatgtcgatc acccgcggca
       61 ccgccagcgg tcgcaggtcg cacacccggc cgccggcgag ctcgaccgcg acgatctggc
      121 tcttgtagtc cgcctcgtcg agcgacagcg gcagcggcgc ccccgcgtag cggatcgcgt
      181 cgcgaccgac ccgctgcgcg cggtggatat ggccgagcgc gacgtagtcg atgtcgtcgg
      241 gaaacagccg cagcggcgcc gactcctggc cgccgatcga cacccggcgc tcgctcaggt
      301 actgcgtgtc cgccccggcg acgtacaggt gcccggtcac gatcagcgcc tgatccggcc
      361 gccgccgcgc ccgcgccgcc gcgatcacct cgccgtagac atcgcccagc ggatcggccg
      421 catcgcccgg cacgtcgacc ggccgcagga acggcaccgc cgcgaccagc ccgcgcccgc
      481 ccgccaccgg gatcaggatc cggtcgagat cgagcccgcc gtccgcgcag cgcggcagcc
      541 cgccgatcac gtgcaccccg agctcgcgca gcaccgacga cgacgcgccg agccgcgccg
      601 gcgagtcgtg gttgcccgcg atcgcgacca cgtccatcgc cggccgcgcc cgccgcaccg
      661 cggccaggaa ctcgaaccac atccgctcgg cgctcgccgg cggcgtcgcc gtgtcgaaca
      721 cgtcccccgt gatcaccagg gcgtcggccg cctcgcgcac gcaggtctcg agcagccagg
      781 ccaggaacgc cgcgtgctcg tactcgcgcg tgacctcgcc ccgcagcgtg tgcccgaggt
      841 gccagtccga cgtgtgcagg agccgcatgc gcgcgcatcc tgccaggggg gtctgacctt
      901 cgggccaccc gtccgggcta ctcgtacgga gcgaacggct ccggcgccgc cggcaacgac
      961 aggcctcgcg aaccgcgccg gcacgcgccg ccgccgagcg aggcggtccg gcggaaccga
     1021 cggccttgct cgtccgctcc tgatacgctc gcctcgatga tcgtgcacac cgatcggctc
     1081 gtgctgcgcc cggtgcaccg cgacgacctc gaggtgctgt gggcgatcca cggcgatccc
     1141 gagaccaacc gctacaaccc cgcgggtccc ctgccctcgc gcaccgccgc cgaggagctg
     1201 ctgcgccagt ggcagtcgca ctggcaggac cacgacttcg gctactgggc gatcgcgacc
     1261 cgcgagcaac cggagaccgt gatcgggttc ggcggagtcc ggctccgcca cttccgggga
     1321 gccgagcgcc tcaacctgtt cttccggctc gcgacctcca cgtggggcaa gggctacgcg
     1381 accgagatgg gccgcgcggc ggtcgcgctc gcattcgacc ggctcggcca cgatgccgtg
     1441 tttgccacca cccggcccga caaccagccc tcgatcgcgg tgctgcagcg cctcgggttc
     1501 cagcgcgatg gcgagctccc cgatgaccgc ggcatgccga gcaacgtcta cgtccgccat
     1561 cgctcgtgac cgcgacgccg aacgccaagc tctgcctgac gccgccgcga gtctcacgat
     1621 ggctcgatcc aggacgccga acgaccttgc ggggatcgat gccgagccct cggacccagt
     1681 tgagagggcg cggcctcact ccctggtctt gcgctcggcc tcgcccggga agtagggctg
     1741 cttcggcccg acctcgtggc cggccggcag cggcacgccc agctcctcga cccggaacac
     1801 ctcgggcggc agccggagcg agtcgcgcac ccggtccatg gtgtccggcg cgaacgggta
     1861 cagcatgatc atcaggttct tgagcacgta gaacgacgca tacagcgcgt cgcggcggcc
     1921 ctcctcgggg cggcggtcgt cgtgcggctt gtactggttg aacaggctgt tgatgatgcg
     1981 cgcgtagttc tcgacctcgt acagcatggt cgggtagtcg gcgcgctcca tgcccttgac
     2041 gtagcgctgg acgatgcgct gggtgtcggt gacgatcttg tcggccagca cgccctgggg
     2101 cacccggccg ccgaacttcg agtgcgcggc ggagatcggc cgctcgaacg tcgcgttcat
     2161 cggcccggcg aggaacgcgt tgcgctcggc gagcttgtcg aagtcgaagt tcgaaggctt
     2221 ctcggacagc ccgagcagcg ccaggtaata ccggatctga tcggcggaat agccctgatc
     2281 gagcagctgg tcgccggtga tgaagttgcc gcgcgacttg ctcatcttct cgccgctgac
     2341 cagcaggtgg aagcagccga agatgtcggt cagctgcaga tcgcccggcg ccggcagccg
     2401 gtgcgggtcg tcctgggtgc cgagccacat cgcgccctgc atcagcacgt agaagaacac
     2461 gttgtcctgg ccgaggaact ggtagacccg cgcgtcggga tcgcgccaga actcggcgta
     2521 gcgctgggcc ggctcgccgc gcgccgcgag cgcgacctgg gagaacgaga tcggcgcgat
     2581 cagcgagtcg ggccagacgt acagcgtctt gcccgccatc tcgggatcga gatcgtcggg
     2641 cagcgggatg ccccagctga cgtcgcgcgt gatcgagcgg tgggcccagt cgtcgtcggg
     2701 cacggtcgcg atcccggcgg cggcgagcgc cgcggccccg gcgtcggcgt cgggccggtt
     2761 cttgaactgc agcacgatgt tcttgcccgc cgtgtacttc atcttgtgcg ccggcaggct
     2821 cgcgcgcagc tccttgtacg cggcctcgcg gtcgccggcc acccgcagcg ccggcagcac
     2881 gcggccgagc acctcgctca gcacccacgg ccgccagctg ccggtcttgc tctcgagcca
     2941 cacccgcagg gtctcggcga ccttggccat gtcgaggaac cagtgcacgg tgtcgcgcat
     3001 caccggcgtg gcgtccgaca gcgcgctctt ggggttgatc agctcgctcg gatcatgctg
     3061 gtggccgcac ttgtcgcact cgtcgctgta cgccgactcg ttgtcgcact tggggttggg
     3121 gcacttgccg cgcacgtagc gatcgggcag gaaccgctcc agcttggggt cgtaccactg
     3181 ccggctcgag cgcttctcga gcatgccgtg gcggtacagc ttgcgcagga agtcctgcga
     3241 catcgccttg tgcagcggga agcactccgg ccgcgccgtc ccggtgtaga cgtcgaggcc
     3301 gatcgaatag cgcttcagcg tggtggcctg ctggtcgtgg acccggtcga gcagctcgcg
     3361 gatcggcacg ccgtcccgca gcgcggcgag ctcgctggtc gagccgtgct cgtcggtgcc
     3421 gcacacgtac agcacgttgg gccggccgat caccatgccg agccagcggg cgtggatgtc
     3481 gcccgggacc tgcgccccgg cgaggtggcc caggtggacc ggcccgttcg cgtagggcat
     3541 gccggcggtg acgactgccc gcttgggccg gtggatgcgc gcgatgatcg acgcgatatc
     3601 gctggccccg cgttcgctgg atgacatccg cgcagactag ccgatgtcgg ccgcccgagc
     3661 accggccggt acacgggacg ccgcggtctg ccaggcggcg tcggcctcag tctggcttgc
     3721 cctggacctc gcgcaggaac gcctcctcgc tcgccgacag cggccggccc ttgagccgct
     3781 ccagctcgtc gcgcgacagc ccgccgacct tctggcggtg ccgcgccatc gcgacgtgcg
     3841 cccgcg
//
LOCUS       DASVOR010000537         5097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_5084,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000537 DASVOR010000000
VERSION     DASVOR010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5097
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5084"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="VF516_10725"
     CDS             complement(<1..517)
                     /locus_tag="VF516_10725"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX8108193.1"
                     /translation="MGARAGFVWAAGRWDWRAGKWEWLPGHWERERPGKRWNPGRWEQ
                     RDGAYVLIDGDWVDGSAAPPPPPGEPPGPPPDDRGDRHREWRLDRPVVSSYWPVKGKI
                     GSRIVIRGRNFPDDTMVMWNGTRVTGAKIEPERIVVAVPPGAESGMLSLRTGRHRELT
                     VGSFEVANYDAE"
     gene            953..1558
                     /locus_tag="VF516_10730"
     CDS             953..1558
                     /locus_tag="VF516_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8108194.1"
                     /translation="MSPHVLDRAARRRVHRRVNRNDLDAVMAAFADDAEYLPGDGKVH
                     RGPAAIRAAFTPQFTGAFGAMRFDPEDRLIDETARKAALRWICRHDLSGPHGTRVAPL
                     LRLAIRLRHGSRVGWHGVDVFHFDAAGKITGKYTYANYDRPRLSRALGVALWHGRTVP
                     RHCMCMAVPSASTAALILRPRCCGLSPATAEPALLRPCGPR"
     gene            2396..3088
                     /locus_tag="VF516_10735"
     CDS             2396..3088
                     /locus_tag="VF516_10735"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX8108195.1"
                     /translation="MATTHDHEYGPPPKTDVTTIAAERVGVTNGLLLKFPQIHSVGDL
                     SLLERPRVAIVGARKATREGRRRAAQLARDCARAGIVVISGLAEGIDHAAHSAAIENG
                     GRTIAVIGTPLDKVYPLNHGALQRKIYREHLLLSPFTWGDKFVPANFPDRNRIMARLA
                     LATVIIEASDTSGSLHQAVESIEVGHPVFIAAGVLDDPKLSWPKRFIGDGQPLGRILR
                     RSADVIEYLISV"
     gene            3914..4363
                     /locus_tag="VF516_10740"
     CDS             3914..4363
                     /locus_tag="VF516_10740"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8108196.1"
                     /translation="MSTSEHQLWSRDTTQKSASVGYQPTDHDPAAQPHAVDIEATVRV
                     SGGDLRVRHCRLRSLSLGGALIEFDRMAPGTLVNLTFGLPTVDERLSLDAVVQGSSKD
                     GVTVLFDSLRAWEVWVLWRFLASLEDDDLDLEPTGEYPVLPLAAAAP"
     gene            complement(4381..>5097)
                     /locus_tag="VF516_10745"
     CDS             complement(4381..>5097)
                     /locus_tag="VF516_10745"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8108197.1"
                     /translation="TAVAAARRATLELVNDAAEPAAFRVERATWSDDALRAGQLLSFQ
                     EFRDLFSEDYIAADVRLGVGEQTLLFTDVVGSTAFYASRGDPAAFVEIKRHFDEVFAI
                     VAANRGAVVKTIGDAVMASFTSPLDAVRAAHQIHDAFHPGRSDTPIRLRISLNTGPCI
                     AVRLNANADFFGGTVNVAAKLQALAEGYQIAMSEATYRSPSVADYLAKQGAELEILQY
                     TSKALPDPVTVRRWTVYREA"
BASE COUNT          738 a         1758 c         1798 g          803 t
ORIGIN      
        1 cctcggcatc gtaattggcg acctcgaagc tgccgaccgt cagctcgcgg tgccggccgg
       61 tgcgcagcga cagcatcccg gactccgcgc ccggcggcac cgccaccacg atgcgctcgg
      121 gctcgatctt ggcgccggtg acccgggtgc cgttccacat caccatcgtg tcatccggga
      181 agttgcggcc gcggatcacg atccggctgc cgatcttgcc cttgaccggc cagtagctgc
      241 tgaccaccgg ccgatccagc ctccattcgc ggtgacgatc gcctcggtcg tcgggcggcg
      301 gccccggtgg ctcgcccggc gggggcggcg gcgcggccga cccatcgacc cagtcgccgt
      361 cgatcaggac atacgcgccg tcgcgctgct cccagcgccc cgggttccag cgcttgccgg
      421 gccgctcgcg ctcccagtgg ccggggagcc actcccactt gccggcgcgc cagtcccagc
      481 gcccggcggc ccacacgaac cccgcgcggg ctcccatgtg ctcgtcttgc ggcggcggcg
      541 gcgcctcgcg cggggagtcc cccggcggcg cctcccggcg atgatcgcgc accaccgggg
      601 gtggcggtgt gcggtggtct cggggttgtg cggagctact cgtcgctccc gcggcgatcc
      661 ccgtcacgac ggtgaccgcg agcaaccatg gttcaacacg tcgcatgcgt tctcctttgc
      721 tgtgagcgtg acgtcgttcc ttgcgacacg agcggtggcg gatccaaaca gtctctttgt
      781 acacaaacaa tgggccggat ccgtcttgcg gtcgcctctc gctggctgag tctgccacga
      841 gcttctcgca cgccgcggtt cgacgcgcca gatcccgccg tattcaacgc cctcgcgtgg
      901 accgtccgcc gcctcgccgg ccgccgccgc cggctcccgc ccgcatggca ccatatcgcc
      961 gcatgtcctc gatcgcgcag ctcgtcgacg cgttcaccga cgcgtcaacc gcaacgatct
     1021 cgacgccgtc atggccgcgt tcgccgacga tgccgagtac ctgcccggcg atggcaaggt
     1081 ccaccgcggc ccggcggcga tccgcgccgc gttcacgcca cagttcaccg gcgcgttcgg
     1141 cgcgatgcgc ttcgatcccg aggaccgcct gatcgacgag accgcccgca aggccgccct
     1201 gcgatggatc tgccgccacg acctgtccgg cccgcacggc acacgcgtgg cgccgctgct
     1261 ccgcctggcg atccggctcc gccacggcag ccgggtcggc tggcacggcg tcgacgtgtt
     1321 tcacttcgac gccgccggca agatcaccgg caagtacacc tacgccaact acgaccgccc
     1381 ccggctgtcc cgcgcgctcg gcgtggcgct gtggcacggc cgcacggtgc cgaggcattg
     1441 catgtgcatg gcggtgccga gtgcatcgac tgcggcgctc atccttcggc cgcggtgctg
     1501 cgggttgagc ccggcaacgg ccgagccggc actgcttcgc ccgtgcgggc cgaggtgaca
     1561 cgttgcgcgt tccatcgcgc agatgcgctg atgtcgatcg cgcagcgtac ctgcgcgcag
     1621 gggacccgct gcagcgcgtg ccgctcgatg tgaccatcac gatcgccgtg ctccttgcgc
     1681 gacgtccccg tttatcgaac cctgccgctc ggaatgcatc gcatgacgtg ctgtcgcctg
     1741 gtgctcgccg cagagcgggc gttatcggag gagcgggaca gattccccgg gcatggcgaa
     1801 gcgcggcgag ctggtgctct atcgggcgca gcggtgctcg gctcactcag gaagatctgt
     1861 gctgcctcca ggccgctgcg agttctgcaa caagggagtt gttcgtcgcg gcgctgctcg
     1921 gcctcgagga tagcgcggcg cgcgcttgac gtcgcctagc gggcgtcgcc ccaccgcacc
     1981 agcaccgcag ccacgtagcc tcgaccgttc tgatgcgcga cgagtcgatg gagggctcgg
     2041 tgaatctttc cggatccgca gatcctggcg caggaggtcg ccgccgaccc gcgatcggcg
     2101 ctcggtccgg tcgaggacat cctcggggac ctcgaagagc gtgccgagac caccagcgga
     2161 caggcacgcg gaacgcggtt acagggtagg ccccgggccg cgggggccgc tgctagccca
     2221 cggcgaggcc agagtacctc gcagcactcc ggcgcagctt cgaacgtcgt agcctgtgga
     2281 tgctccccag agacagcggg cgcatggccg ggcgctgctt cgctctgatc ttgcaccgat
     2341 atccagggca tggacttatg tcagacccca cagctacgtt cagcggatcc tggtgatggc
     2401 cacgacgcac gaccatgagt atggcccgcc gccgaagacg gacgtgacga cgatcgctgc
     2461 cgagcgtgtg ggggtcacga acggcttgct gctcaagttc ccgcagatcc acagcgtggg
     2521 cgatctctcc ttgctggagc gccctcgcgt tgcgatcgtc ggtgcacgga aggcgacgcg
     2581 ggaaggccgt cgccgcgctg cgcagctcgc cagagattgt gcgcgcgccg gcatcgtcgt
     2641 gatttccggg cttgcagagg ggatcgacca cgctgcgcat tcggctgcga tcgagaatgg
     2701 gggccggacg atcgcggtga tcggaacacc gctcgacaag gtctacccgc tgaaccacgg
     2761 tgcgcttcag cgcaagatct atcgcgagca cctgctgctc tcgccgttca cctgggggga
     2821 caagttcgtc cctgcaaatt tcccggaccg caaccggatc atggctcgcc tggcgctcgc
     2881 gacggtgatc atcgaggcga gcgacaccag tgggtcgctt caccaggccg tggagagcat
     2941 cgaggtcggt catcctgtct tcatcgcggc cggggtgctc gatgatccga agctgagctg
     3001 gccgaagcgc ttcatcgggg acggtcagcc gctcgggcgg atcctgcgcc ggagtgccga
     3061 cgtgatcgag tacctgatct ccgtgtgaat catgaggacc atcggcggcg tggcgctggg
     3121 gacctactat gcggccggtg cgcaagcagc caaggcgacc ccgaacgcct cggcgatgcg
     3181 cggcctggtg tacgccgtga aggacaaccg cgatcgtcgc ccaggttgcg agcgagctgc
     3241 ggaagcacgg tgcccgtccg gggctgccgg gcagggcgaa cacgggcagc cggcgacgtg
     3301 tgccgccggc tgcgctacgc aggcagcacg ggctgcctac ctggagtgaa tcccgccgcc
     3361 ggcgtggacc ggtccgacgc gggcactcgc gtggcaggca gcgaacccga gtgcggcgag
     3421 acacgagatg attactttgc ggaacatgat cattacctcc gattgtctct ggcagggatc
     3481 agtgcagatg ccgggccaga gtgacctcgg cgcacgggga gcttccatgt cggcggaacc
     3541 tccacgcgcg cgcgccgcgg tctcgccggt tacgccgcgg ctcaggcacg cgctgtcgga
     3601 gaccgttcga tgcgtgagct gaccgggccg tcacaggggg cgggccgacc tgcgatgggc
     3661 gccgggcgcg cgacgtcagg tgcgcctgac gcagcggcct cgatcgcatg cgcgtcctgc
     3721 accggtgtgg ccgtgatctg gacgtgagac gctcgtgaga acgaagactt agaccatcgg
     3781 gaccgtccag gtgaacatcg gccagaccag gggccgctta cgcgttttcc tcgggaatag
     3841 gccgatgtca ccggagctcg gggtctgccg acgaggatcg agcgggcacg cacgatgcag
     3901 aaacggctcg gtgatgagca cctccgagca ccagctctgg tcgcgcgaca cgacgcagaa
     3961 gtccgcgtcc gtgggctacc agccgaccga tcacgacccc gccgcccagc cgcacgccgt
     4021 ggacatcgaa gcgaccgttc gcgtttccgg cggcgacctt cgcgtgcggc actgccggct
     4081 gcgcagcctg tcgctcggcg gtgcgctgat cgagttcgac cgcatggcgc ccgggacgct
     4141 ggtcaacctc acgttcggcc tgccgacggt cgacgagcgg ctgtcgctcg atgcggtcgt
     4201 ccagggcagc tcgaaggacg gcgtgaccgt gctgttcgac agcctgcgcg cctgggaggt
     4261 ctgggtgctg tggcggttcc tcgcgtcgct cgaggacgac gacctggatc tggagccgac
     4321 cggggagtat ccggtgctgc cgctggcggc ggccgcgccc tagcgggtct ttcccacgcc
     4381 tcaggcctcg cggtagaccg tccagcggcg aacggtgacc ggatcgggca gggccttgga
     4441 cgtgtactgg aggatctcga gctcggcgcc ttgcttggcg aggtagtctg ccacgctggg
     4501 cgagcggtag gtcgcctcgc tcatcgcgat ctgatagccc tcggcgagcg cctggagctt
     4561 ggccgcgacg ttgacggtgc cgccgaagaa gtcggcgttg gcgttgagcc ggacggcgat
     4621 gcacgggccg gtgttgagcg agatgcgcag ccggatcggc gtgtcgctgc ggccggggtg
     4681 gaaggcgtcg tggatctggt gcgcggcgcg gacggcgtcg agcgggctgg tgaacgaggc
     4741 catcacggcg tcgccgatgg tcttgaccac cgcgccgcgg ttcgccgcga cgatcgcgaa
     4801 cacctcgtcg aagtgccgct tgatctcgac gaacgcggcg gggtcgccgc gcgaggcgta
     4861 gaacgcggtg gagccgacga cgtcggtgaa cagcagggtc tgctcgccga cgccgagccg
     4921 gacgtcggcc gcgatgtagt cctcggagaa caggtcgcgg aactcctgga agctgagcag
     4981 ctggccggcg cgcagcgcat cgtcgctcca ggtcgcgcgc tcgacccgga acgcggccgg
     5041 ctcggcggcg tcgttgacca gctccagggt ggcgcgccgg gccgcggcga ccgcggt
//
LOCUS       DASVOR010000538         3569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_560444,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000538 DASVOR010000000
VERSION     DASVOR010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560444"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..707
                     /locus_tag="VF516_10750"
     CDS             <1..707
                     /locus_tag="VF516_10750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108198.1"
                     /translation="LAKAPEAVLPVTRALLATGDEQVARRYAGVLRSHRGHVPGPAID
                     ELVDRARDDLERQARGKATADELVVARVLAELIADLAPARHVELLFERARRLRKAGKS
                     VEAFGSLKPLLRSRADIDGAIDDEQRFLLAVLALETVGDSLIRTARTDDPVFEQFARL
                     AGKGFPVARRLAREHDVSDEAIYALGFRLLESTAGGHEDLGAELLQGIIDERPRSKLA
                     KAARNKLKLTGHLDED"
     gene            complement(709..1413)
                     /locus_tag="VF516_10755"
     CDS             complement(709..1413)
                     /locus_tag="VF516_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8108199.1"
                     /translation="MSDADDTSKKRILVIEDEPDIVRGLRDALEFEGFEVQARGTGAE
                     GLAAALDWGPDAVLLDLMLPDDNGYRVCETLRQRDQTVPIIILTAKAQESDKIRGLDA
                     GADDYVTKPFSVAELIARINAIFRRQARMAHSDETFLIGSWQINSRKHTMSRGRTSKR
                     LTFYELEVLKLLRERRDETVSRDELLEKVWGIQAAPTSRTVDNFIVKLRRKLEEDQKS
                     PRHILTVYGLGYKLVP"
     gene            complement(1470..2993)
                     /locus_tag="VF516_10760"
     CDS             complement(1470..2993)
                     /locus_tag="VF516_10760"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8108200.1"
                     /translation="MRRVLLVNALIFAVLAAAVALAYYGYSYTSEVSSSERELEVLQE
                     LADDKVLDIESRIESSDNKLLAGVQLEPMSDLRELVKTTGVAATSVFVLDDHLALVPD
                     GSVSTRLGKPGIEFRDKFLARIVPKLPLARQPVGKRGHVYGTWDGRPYLFSFMKRVAG
                     DKTFYVVIEDDLIHLVNNVFPQFFSVAGSSKRLYQVRDETGALRYGAPFGDTAGEPVQ
                     ETRFVSTVDGWVLRVTQKDMTQPELDRRKLIDSLLIGVAVTVIVAGLTFLGLTVRRER
                     RLNELKSEFISNVSHELKTPLSIISMFGEMLAEGRTKSPEQAHEYAEIIWRESVRLGR
                     LIDNVLDFAKIERGMGVYEFAEADLGEVIERVVELSARRVAAADMTLEADIAPDLPVM
                     QLDANAFTLAVLNLIDNAIKYAAAGKRIALAVRCDDGRVELTVRDWGPGIDPEEHDRI
                     FDRFYRARAIRLAPIRGSGIGLALVQHIARAHGGDASVTSVPGQGATFRLSLPVIEP"
     gene            3167..>3569
                     /locus_tag="VF516_10765"
     CDS             3167..>3569
                     /locus_tag="VF516_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108201.1"
                     /translation="MDSIFEEDADEVGGEIEAYCPSPRCKADTTHTIISMYEDEVRRV
                     QCVVCGEVHAYRKPRGDGIEEAADGAAKKSSQRRMTWDDAMARATDADLNNCRPYSIR
                     DAYEEGDVVHHPVFDIGFVTELLPDNKVEVIF"
BASE COUNT          562 a         1229 c         1221 g          557 t
ORIGIN      
        1 gcctggccaa ggcgcccgag gcggtgctcc cggtcacccg cgcgctgctg gccaccggcg
       61 acgagcaggt cgcgcggcgc tacgccggcg tgctgcgctc gcaccgcggc cacgtccccg
      121 gcccggcgat cgacgagctc gtcgaccgcg cgcgcgacga cctggagcgc caggccaggg
      181 gcaaggcgac cgccgacgag ctggtcgtcg cgcgcgtgct cgccgagctg atcgccgacc
      241 tcgcgccggc ccgtcacgtc gagctgctgt tcgagcgcgc ccgccggctg cgcaaggccg
      301 gcaagtcggt cgaggcgttc ggcagcctca agcccctgct ccggtcgcgc gccgacatcg
      361 acggcgcgat cgacgacgag cagcggttcc tgctcgccgt gctggcgctc gagacggtcg
      421 gcgacagcct gatccgcacc gcgcgcaccg acgatccggt gttcgagcag ttcgcgcggc
      481 tcgccggcaa gggcttcccg gtcgcccggc ggctcgcccg cgagcacgac gtgtccgacg
      541 aggcgatcta cgccctcggc ttccgcctgc tggagtccac tgccggcggc cacgaggacc
      601 tcggcgccga gctcctgcag ggcatcatcg acgagcgccc gaggagcaag ctcgcgaagg
      661 cggcgcgcaa caagctcaag ctgaccggtc acctcgacga ggactaggct acggcaccag
      721 cttgtagccc aggccgtaga ccgtgaggat gtggcgcggg ctcttctgat cctcctcgag
      781 cttgcggcgc agcttgacga tgaagttgtc gacggtccgg ctggtcggcg cggcctggat
      841 gccccacacc ttctcgagca gctcgtcgcg cgacacggtc tcgtcgcggc gctcgcgcaa
      901 cagcttcagg acctcgagct cgtagaacgt gagccgcttg ctggtccggc cgcggctcat
      961 cgtgtgcttg cgcgagttga tctgccagct gccgatcagg aaggtctcgt cgctgtgggc
     1021 catgcgggcc tggcggcgga agatcgcgtt gatccgcgcg atcagctcgg cgaccgagaa
     1081 cggcttggtc acgtagtcgt cggcgccggc gtccaggccg cggatcttgt cgctctcctg
     1141 ggccttggcg gtgaggatga tgatcggcac ggtctgatcg cgctgccgca aggtctcgca
     1201 cacgcgatag ccgttgtcgt cggggagcat cagatcgagc agcaccgcgt cgggccccca
     1261 gtccagcgcg gcggccaggc cctcggcgcc ggtgccgcgc gcctggacct cgaagccctc
     1321 gaactccagc gcgtcgcgca gcccgcgcac gatgtcgggc tcgtcctcga tcaccaggat
     1381 ccgcttcttc gaggtgtcgt ccgcgtcgga cactggtaca ctagtaccag agatcaccgg
     1441 gccgcgcgac gcggggtcgg acgcaggggt cagggctcga tcaccgggag cgagaggcga
     1501 aacgtcgcac cttgccccgg aacgctggtc accgacgcgt cgccgccgtg cgcgcgcgcg
     1561 atgtgctgga ccagcgccag gccgatgccc gagccccgga tcggtgccag ccggatcgcc
     1621 cgcgcgcggt agaaccgatc gaagatccgg tcgtgctcct cggggtcgat ccccgggccc
     1681 cagtcgcgca cggtgagctc gacgcgaccg tcgtcgcagc gcaccgccag cgcgatccgc
     1741 ttgcccgccg cggcgtactt gatcgcgttg tcgatcaggt tgagcacggc gagcgtgaac
     1801 gcgttggcgt cgagctgcat caccggcagg tcgggcgcga tgtcggcctc gagcgtcatg
     1861 tcggcggcgg cgacccggcg ggccgacagc tcgaccacac gctcgatgac ctcgccgagg
     1921 tcggcctcgg cgaactcgta gacccccatg ccgcgctcga tcttggcgaa gtcgagcacg
     1981 ttgtcgatca gccggccgag ccgcacgctc tcgcgccaga tgatctcggc gtactcgtgc
     2041 gcctgctcgg gactcttggt ccggccctcg gccagcatct cgccgaacat cgagatgatc
     2101 gacagcgggg tcttgagctc gtgcgacacg ttggagatga actcgctctt gagctcgttg
     2161 agcctgcgct cgcggcggac cgtcaggccg aggaaggtca gccctgcgac gatcacggtc
     2221 accgcgacgc cgatcagcag cgagtcgatc agctttctgc ggtcgagctc gggctgggtc
     2281 atgtccttct gggtcacccg cagcacccag ccgtcgaccg tcgacacgaa ccgggtctcc
     2341 tggaccggct cgcccgcggt gtcgccgaac ggcgcgccgt agcgcagcgc gccggtctcg
     2401 tcgcggacct gatagagccg cttggacgac cccgccaccg agaagaactg cgggaacacg
     2461 ttgttgacca ggtggatcag gtcgtcctcg atcacgacgt agaacgtctt gtcgcccgcg
     2521 acccgcttca tgaacgagaa caggtacggc cggccgtccc acgtgccgta gacgtggccg
     2581 cgcttgccga ccggctgcct ggcgagcggc agcttgggga cgatccgggc caggaacttg
     2641 tcgcggaact cgatgcccgg cttgccgagc cgggtcgaca ccgagccgtc cggcaccagc
     2701 gcgaggtggt cgtcgagcac gaacacgctg gtcgcggcga ccccggtggt cttgaccagc
     2761 tcgcgcaggt ccgacatcgg ctcgagctgc acccccgcga gcagcttgtt gtcgctgctc
     2821 tcgatccgcg attcgatgtc gaggaccttg tcgtcggcca gctcctggag cacctcgagc
     2881 tcgcgctcgc tggacgacac ctcgctggtg taggagtagc cgtagtaggc gagcgccacc
     2941 gccgcggcca acaccgcgaa gatcaaggcg ttcacgagca gaacgcggcg cacgtggggg
     3001 gatcctgcca tgttctgtat cagttgtaag tgtgcaaggt tgctagctca cgggatcatg
     3061 tgttagaggg ctaactaata cgtcgagcaa tccggacttc cggatttccc gccgtttcaa
     3121 gtacttaggc tagtaggcgg cgacactcgt ccaaaagagg catgggatgg attccatctt
     3181 cgaagaagac gccgatgaag tgggcggaga gatcgaggcg tactgcccgt ccccccgctg
     3241 caaggccgac acgacccaca ccatcatcag tatgtacgag gacgaagtcc gccgcgtgca
     3301 gtgcgtggtg tgcggcgagg tccacgcgta cagaaagccg cgcggcgacg ggatcgagga
     3361 ggccgccgac ggcgccgcca agaagtcgtc gcagcggcgg atgacgtggg acgatgcgat
     3421 ggcgcgcgcg accgacgccg acctcaacaa ctgccggccg tactcgatcc gcgacgccta
     3481 cgaggaaggc gacgtcgtcc accacccggt gttcgacatc ggcttcgtca ccgagttgct
     3541 tccggacaac aaggtcgagg tgatcttcc
//
LOCUS       DASVOR010000539         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_560454,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000539 DASVOR010000000
VERSION     DASVOR010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560454"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VF516_10770"
     CDS             <1..191
                     /locus_tag="VF516_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108202.1"
                     /translation="IDWFSSAAMFTGWAEYGDEPFLWRGPPTYDPLIGYQPRTWLLRE
                     GWKKSGSISCREVPSAAH"
     gene            complement(276..713)
                     /locus_tag="VF516_10775"
     CDS             complement(276..713)
                     /locus_tag="VF516_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108203.1"
                     /translation="MNFEDVDAFAMNLPGVTVGLKWGKRTWMVDGKGFAWQRPFSKAD
                     LKRFGNEPPPAGEILAVLVENLDAKDALLAIAPPGFFTIPHFDGYPAVLIALRQARTK
                     DVRAAILDAFRTVASSRPIAKHAAPRARKRRGVPARRARATSR"
     gene            complement(749..>1573)
                     /locus_tag="VF516_10780"
     CDS             complement(749..>1573)
                     /locus_tag="VF516_10780"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HEX8108204.1"
                     /translation="LDADTGVSPASLDAALRAAGALVNAVDAVLDEKATRVFCAVRPP
                     GHHATRDRAMGFCLLNNVAAAAAAARAGGAARVAILDWDVHHGNGTQDIFWNDPNVLY
                     LSVHQFPFYPGTGAATELGGTSAIGATVNVGLPAGCGDAEYAAVFDHVFLPKLAMFRP
                     DILLISAGFDAFQHDPLAGMRVTLPGFAAMAHRLRASADRWSAGRIVAVLEGGYDLDG
                     LGGGMTAVLSAFADPPQPLPALAPLPAHMVAKAAIEGTLAAHRNAGAAIPDPTTAR"
BASE COUNT          247 a          516 c          604 g          206 t
ORIGIN      
        1 cgatcgactg gttctccagc gcggcgatgt tcaccggctg ggcggagtac ggcgacgaac
       61 cgttcctgtg gcgcggccca ccgacctacg acccgctgat cgggtaccag ccgaggacct
      121 ggctgctccg cgagggctgg aagaaatccg gatcgatctc ctgccgcgag gtcccgtcgg
      181 ctgcgcactg agcgccgaat acaaccgccc tcgagcgctc gacagctcgg cgcgcggcgg
      241 ctcgccgacg agaccgggca cgcccaagtc gacggtcaac gcgacgtcgc acgtgcccgt
      301 cgcgccggaa cgccgcgccg cttgcgggcg cggggagcgg cgtgcttcgc gatcggccgc
      361 gacgacgcga cggtgcgaaa cgcgtccagg atcgcggcac gcacgtcctt cgtacgcgcc
      421 tgccgcaacg cgatcaggac cgcggggtaa ccgtcgaaat gaggaatcgt gaagaaaccg
      481 ggcggcgcga tcgcgagcaa cgcatccttg gcatcgagat tttcgaccaa aacggcgagg
      541 atctcgcccg ccggtggcgg ctcgttacca aaccgcttga gatcggcctt gctgaacggc
      601 cgctgccacg cgaagccctt gccgtcgacc atccacgtgc gcttgcccca cttcaagccg
      661 accgtgacgc cggggaggtt catcgcaaac gcatcgacat cctcgaagtt catggcaatg
      721 gctggtgcga gtttgtaccg gccagcggct accgcgccgt ggtcggatcg gggatggcgg
      781 cgccggcgtt gcggtgcgcg gccagcgtgc cctcgatcgc ggccttggcg accatgtggg
      841 ccggcagcgg agcgagtgcc ggcagcggct ggggcggatc ggcgaacgcc gacaggacgg
      901 cggtcatgcc gccaccgagg ccgtcgaggt cgtagccgcc ctcgaggacc gcgacgatgc
      961 ggccggccga ccagcgatcg gcggacgcgc gcaggcggtg ggccatcgcg gcgaacccgg
     1021 ggagggtgac gcgcatgccg gcgaggggat cgtgctggaa ggcgtcgaac ccggcgctga
     1081 tcaggaggat gtcggggcgg aacatcgcga gcttggggag gaacacgtgg tcgaacaccg
     1141 cggcgtactc ggcgtcgccg cagccggcgg ggaggccgac gttgacggtg gcgccgatcg
     1201 ccgaggtccc gccgagctcg gtcgcggcgc cggtgcccgg gtagaacggg aactggtgga
     1261 ccgagaggta gagcacgttg gggtcgttcc agaagatgtc ctgggtgccg ttgccgtggt
     1321 ggacgtccca gtcgaggatc gcgacgcggg cggcgccccc ggcgcgcgcg gcggcggcgg
     1381 ccgcggcgac gttgttgagc aggcagaagc ccatggcgcg atcgcgcgtg gcgtggtggc
     1441 cgggcgggcg tacggcgcag aaaacacgcg tggccttttc gtccagcacg gcatcgacgg
     1501 cgttcaccag cgcgccggcg gcgcgcagcg cggcgtcgag cgaggcgggc gacacgccgg
     1561 tatcggcatc gag
//
LOCUS       DASVOR010000540         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_209550,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000540 DASVOR010000000
VERSION     DASVOR010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209550"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="VF516_10785"
     CDS             <1..389
                     /locus_tag="VF516_10785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108205.1"
                     /translation="LLERLADDDAAAFVVAHELAHHRLGHVPALPRSWLALGRLLLVA
                     LERWWIATPEHEHDADQLAIELCVDAGYDAGRCIAALEHLVNVSLDYGDVEGVLGDED
                     GIQRGSHPALVRRIAAVRARLAALRG"
     gene            535..1002
                     /locus_tag="VF516_10790"
     CDS             535..1002
                     /locus_tag="VF516_10790"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8108206.1"
                     /translation="MTTSPAKVTRTSRTFRIECSAAIDIRATPQRIWSLLTNAADFPR
                     WNSTVTRMSGTIAEGERLALEVAAAPGRTFRPRVTRIEPEKLMVWSDGFAPMFKGVRT
                     FALAPAGDGKTAFSMVEVLSGVMLPMIKGSLPDFGPSFRAYAADLQREAERAA"
     gene            1090..>1558
                     /locus_tag="VF516_10795"
     CDS             1090..>1558
                     /locus_tag="VF516_10795"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8108207.1"
                     /translation="MEPAPAGFAQAREQFLAMVGAIRPELHRYCARLTGSVIEGEDIV
                     QDTLARAFYALSMMPEVPPLRPWLFRIAHNAAIDFLRRHGHKYTEARDDLDDAIGFED
                     RADPAVVRAALSRFVVLPVSQRSAVILKDVLGHSLDDAAAVMGATVPAVKAALV"
BASE COUNT          210 a          532 c          583 g          233 t
ORIGIN      
        1 ggttgctcga gcggctcgcc gatgacgatg ccgcggcgtt cgtcgtcgca cacgagctcg
       61 cgcaccaccg gctcggccat gtcccggcgc tgccgcgcag ctggctcgcc ctggggcggt
      121 tgctgctggt cgcgctcgaa cgctggtgga tcgcgacgcc ggagcatgag cacgacgccg
      181 atcagctggc gatcgagctg tgcgtcgatg ccggctacga cgccgggcgc tgcatcgccg
      241 cgctggagca tctcgtgaac gtctcgctcg actacggcga cgtcgagggc gtgctcggcg
      301 acgaggacgg gatccagcgg ggaagccacc cggcgctggt ccgtcggatc gccgcggtgc
      361 gggcgcgcct ggcggcgctg cgggggtagc cctcggacgc ggcctgccgg gctcgcgact
      421 ccgcggctgc gtgaggcgaa gcttcggcgg cgggccgcct gggattccgc acagttcgca
      481 gtttttcacc cgcgaccgca tcctttcggc cggctcgccc gtcgtccggc cgagatgacg
      541 acttcccccg ccaaggtcac ccggacgtcg cggacgttcc gcatcgaatg ctcggctgcg
      601 atcgacatcc gcgccacccc gcagcgcatc tggtcgctgc tcaccaacgc cgcggacttc
      661 ccgcgctgga actccacggt cacccggatg tccggcacga tcgccgaggg cgagcggctg
      721 gcgctcgagg tcgcggcggc gccgggccgg acgttccggc cccgggtgac gcggatcgag
      781 ccggagaagc tgatggtgtg gagcgacggg ttcgcgccga tgttcaaggg cgtgcggacg
      841 ttcgcgctcg caccggcggg cgatggcaag acggcgttct cgatggtcga ggtgctgagc
      901 ggcgtgatgc tgccgatgat caagggctcg ctcccggact tcggcccgtc gttccgggcg
      961 tatgcggccg acctccagcg cgaagcggaa agggccgcgt gatggcgaag atcaagggac
     1021 aggacaaccc gcggaacaac cggatgcgcg ggctcggcaa caagcggtga gccggtttat
     1081 cgtgccggca tggaaccggc accggcgggc ttcgcccagg cgcgcgagca gttcctcgcg
     1141 atggtcggcg cgatccgccc cgagctgcac cgctactgcg cccggctgac cggatcggtg
     1201 atcgagggcg aggacatcgt gcaggacacg ctggcccggg cgttctacgc gctgtcgatg
     1261 atgcccgagg tgccgccgct ccggccgtgg ttgttccgga tcgcgcacaa cgcggcgatc
     1321 gacttcctgc gccgccacgg ccacaagtac accgaggcgc gcgacgacct cgatgatgcg
     1381 atcggattcg aagaccgcgc cgatccggcg gtggtgcgcg ccgcgctgtc gcggttcgtc
     1441 gtgctgccgg tgtcgcagcg cagcgcggtg atcctgaagg acgtgctggg ccactcgctg
     1501 gacgacgccg cggcggtgat gggcgcgacg gtgcccgcgg tcaaggcggc gctggtcc
//
LOCUS       DASVOR010000541         2590 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_326450,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000541 DASVOR010000000
VERSION     DASVOR010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2590
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326450"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1729
                     /locus_tag="VF516_10800"
     CDS             221..1729
                     /locus_tag="VF516_10800"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8108208.1"
                     /translation="MTNELDTLRSERDFYRSLAELTEHDRLPALLEAALGVLIARVSA
                     REAMIEVIDDGIDGASHVVAHGCERDRLTEISSFVSRGIIGEAMATGKTIVTANAAED
                     PRFLQFESVQRHQLEAVLCVPIGQAVHAGVVYLQGKQGAREFQPYDKKVQREVELFAR
                     VLSGPVDRLLGRGPAALAAPPHSRSADDPFAAIRGKSPAMADIVERLRLAAPLDVHVL
                     FTGPSGVGKTLLANAVHLASRRRNGPFVEINCATLPEALLENELFGAEPGAHSAATRG
                     AIKGKVEAAEGGTLFLDEIGELTPGAQSKLLQLLQNKTYYRLGSTLARRADVRVVAAT
                     NANLKAAIAERRFREDLYYRLQVLEVHVPSLAERPEDVVPLSFEFLRQVVERHQLTRK
                     SLSPSAIRAIQAAQWPGNVRQLANKIESAAIHAQIRGSDHIEAHDVFPDDPQSASDAP
                     ATLQSATRRFQRNHILGVLTATDWNALEAARILDVSRSQIYNLIRMFELKSG"
     gene            1917..>2590
                     /locus_tag="VF516_10805"
     CDS             1917..>2590
                     /locus_tag="VF516_10805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108209.1"
                     /translation="MHELAPGLDIAVAPTIAWIQTLHVDLVGRLPDRRALVRWIAPSR
                     GASHHEIARDLLRSEDYCRAQIAALYRSLLDRDGDPESVAAWMRSLQSGIAIQDVIAS
                     ICDGFEYKTNHPGGPALVESLYRRLLHRASDPEGRTAALAALRQRSSTLAVIRGFLTS
                     TEYCCQRVTELYVGLLGREPEPTALSEQSLALMHGTPLQQIALDLVTSDEYIARAAHR
                     KAELAAL"
BASE COUNT          399 a          948 c          874 g          369 t
ORIGIN      
        1 accgcgacgg ccgggagcgg gcgggcgcgg acggcgcgac gctgcaccgg ctcggcctgg
       61 tacccgatgg cgatgcgttg acgcggtggt ccaccggcca ggcgctgcgg gcgatcgatc
      121 gggtgtacgt cgcgtgacgc cgcgtgccga tgcgtgatcg aggccgcggc cggcggcaaa
      181 actctaccat cgaccggcgg tggtccgtta ggctgcgcgc gtgacgaacg agctcgacac
      241 cctgcggagc gagcgcgact tctatcgatc gctcgccgag ctcaccgagc acgaccgcct
      301 gcccgccctg ctcgaggccg cgctcggcgt gctgatcgcc cgcgtcagcg cgcgcgaggc
      361 gatgatcgag gtgatcgacg acgggatcga cggcgcctcc cacgtcgtcg cacacggctg
      421 cgagcgcgac cgcctgaccg agatctcgtc gttcgtgtcg cgcggcatca tcggcgaggc
      481 gatggcgacc ggcaagacga tcgtcaccgc gaacgccgcc gaggatccgc ggttcctcca
      541 gttcgagagc gtccagcgcc accagctcga ggccgtgctg tgcgtgccga tcggccaggc
      601 cgtgcacgcc ggcgtggtct acctgcaggg caagcagggc gcccgcgagt tccagcccta
      661 cgacaagaag gtccagcgtg aggtcgagct gttcgcgcgc gtgctgagcg gcccggtcga
      721 tcgcctgctc ggccgcggcc cggccgcgct ggccgcgccg ccgcactccc gcagcgccga
      781 tgatccgttc gccgcgatcc gcggcaagag cccggcgatg gccgacatcg tcgagcgcct
      841 ccggctggcg gcgccgctcg acgtccacgt gctgttcacc ggaccatccg gagtcggtaa
      901 gacgttgctc gccaacgcgg tccacctggc gtcgcgccgg cgcaacggtc cgttcgtcga
      961 gatcaactgc gcgacgctgc ccgaggcgct gctggagaac gagctgttcg gcgccgagcc
     1021 cggggcccac tcggcggcga cccgcggcgc gatcaagggc aaggtcgagg ccgccgaggg
     1081 cgggaccctg ttcctcgacg agatcggcga gctcaccccg ggcgcgcagt ccaagctgct
     1141 ccagctgctc cagaacaaga cctactaccg gctcggcagc acgctggcgc gccgcgccga
     1201 cgtccgggtc gtcgccgcga ccaacgccaa cctcaaggcc gcgatcgccg agcgcaggtt
     1261 ccgcgaggac ctgtactacc gcttacaggt gctcgaggtc cacgtcccgt cgctcgccga
     1321 gcgccccgag gacgtcgtgc cgctcagctt cgagttcctg cgccaggtcg tcgagcgcca
     1381 tcagctgacc cgcaagtcgc tcagcccgag cgcgatccgt gcgatccagg ccgcccagtg
     1441 gcccggcaac gtccggcaac tcgccaacaa gatcgagtcc gcggcgatcc atgcccagat
     1501 ccgcggcagc gaccacatcg aggcccacga cgtgttcccc gacgatccgc agtcggcgag
     1561 cgacgccccg gcgacgctgc agagcgcgac ccggcggttc cagcgcaacc acatcctggg
     1621 cgtgctcacc gccaccgact ggaatgcgct ggaggcggcg cggatcctcg atgtgtcgcg
     1681 atcgcaaatc tacaacttga tccgaatgtt cgagctcaag agcggctgat ccccggatcg
     1741 accgtccacg acagtccacg aatgtggcct gccagggcgc cgcagtcctg atccgtggac
     1801 tgcgcgagcg tcccgcgatc gccggcgcac gccgcgacgc gagttagcgc tcgccgccga
     1861 tggcacgacg gctgctcgac gctgtcgccg tgctcgtcga ccggaggccc aagaggatgc
     1921 atgagctcgc tcccggactg gacatcgctg tcgcgccgac gatcgcctgg atccagacac
     1981 tgcatgtcga cctggtgggc cggcttccag atcgccgcgc actcgttcgc tggatcgcgc
     2041 cctcgcgggg cgcctcgcat cacgagatcg cgcgcgatct gctgcgatcc gaggactatt
     2101 gccgcgccca gatcgccgcg ctgtatcgct cgctgctcga tcgcgatggc gatccggaga
     2161 gcgtcgcggc gtggatgcgc tcgctgcaat cggggatcgc gatccaggac gtgatcgcca
     2221 gcatctgcga cggcttcgag tacaagacca accacccggg tggcccggcg ctcgtcgagt
     2281 cgctgtaccg ccgcctgctc caccgcgcct ccgatccaga aggcaggact gccgcgctcg
     2341 ccgcgctgcg acagcgatcc agcacgttgg cggtgatccg aggatttctg acgtcgaccg
     2401 aatactgctg ccagcgggtc accgagctat atgtaggtct actcggcaga gagccggagc
     2461 cgaccgcgct gtccgagcag tccctcgcgc tgatgcacgg cacgccgctg cagcagatcg
     2521 cgctcgacct ggtgacctcg gacgagtaca tcgcgcgcgc cgcgcaccgc aaggccgagc
     2581 tcgccgcgct
//
LOCUS       DASVOR010000542         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443226,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000542 DASVOR010000000
VERSION     DASVOR010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443226"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VF516_10810"
     CDS             <1..1228
                     /locus_tag="VF516_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX8108210.1"
                     /translation="VLWLFFGDSHTAGDSLTSRLRVTLQSKFGDAGRGLVAAGRPPTR
                     HYYQRDVRYGTTGKWKAAVGGHKRDPEPYGLAGLRVYGERKGAELWVETCSDCQAGTR
                     VAQFEILYYAAPDHGLLRYRVDDQPWQPLKTRTTAIEPPHPARQVIPVTDGPHKLTLQ
                     HGGGGVIDLFGVVMERLRPGVIVDSLGVVGRRLGSLRSWDWSVIGDQLASRDPRLVVL
                     QYGTNEADDPDLDLEAMARYFDETILRIRAAAPTAAVLVLGPPDMATREAGKHCDRMK
                     PDPLHPDAPVIPECEWHTPHVLAEIIAVEHAAALRNRAAFFDTFAAMGGADRMHAWFL
                     AEPRLAYKDHVHLTDAGYQQWADVLSQAVLAEYARWRKRLDLPPTPPLIAPPPAPPSD
                     AASPGAPPPDPGPDRP"
     gene            1238..>1522
                     /locus_tag="VF516_10815"
     CDS             1238..>1522
                     /locus_tag="VF516_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108211.1"
                     /translation="MLFSSPDYPLFLIAVFFLYALARWGGPAMAWARICVMVLLGDVV
                     FALVAKDPDALWDPIGGVLLRLAAGAGAVVGSSAPAGSPAAIGVLAVHWAI"
BASE COUNT          185 a          561 c          557 g          219 t
ORIGIN      
        1 cgtgctgtgg ctgttcttcg gcgactcgca cacggccggc gactcgctga cctcgcggct
       61 ccgggtcacg ctgcagagca agttcggcga cgccgggcgc ggcctggtcg ccgccggccg
      121 gccgccgacc cggcactact accagcgcga cgtgcgctac gggacgaccg gcaagtggaa
      181 ggcggcggtc ggcggccaca agcgcgaccc cgagccgtac gggctggccg ggctgcgggt
      241 ctacggcgag cgcaagggcg ccgagctgtg ggtcgagacc tgcagcgatt gccaggccgg
      301 gacccgggtc gcccagttcg agatcctgta ctacgccgcg cccgatcacg gcctcctgcg
      361 ctaccgggtc gacgaccagc cgtggcagcc gctcaagacc cggaccacgg cgatcgagcc
      421 gccgcacccg gcgcgccagg tgatcccggt gaccgacggc ccgcacaagc tcacgctgca
      481 gcacggcggc ggcggcgtga tcgacctgtt cggcgtcgtg atggagcggc tgcggccggg
      541 cgtgatcgtc gactcgctcg gcgtggtcgg ccgccggctc ggcagcctgc ggtcgtggga
      601 ctggtcggtg atcggcgacc agctggcgag ccgcgatccg cgcctggtcg tcctgcagta
      661 cggcaccaac gaggccgacg atccggacct cgatctcgag gcgatggcgc gctacttcga
      721 cgagaccatc ctgcggatcc gcgcggccgc gccgaccgcg gcggtgctgg tgctcggccc
      781 gcccgacatg gcgacccgcg aggccggcaa gcactgcgac cggatgaagc ccgatccgct
      841 gcaccccgac gcgccggtga tccccgagtg cgagtggcac acgccgcacg tgctcgccga
      901 gatcatcgcg gtcgagcacg ccgcggcgct gcgcaaccgc gccgcgttct tcgatacgtt
      961 cgccgcgatg ggcggcgccg atcgcatgca cgcatggttc ctcgccgagc ccaggctcgc
     1021 ctacaaggat cacgtccacc tgaccgacgc cggctaccag cagtgggccg acgtgctgtc
     1081 ccaggcggtg ctcgccgagt acgcgcgctg gcgcaagcgg ctcgacctgc cgccgacccc
     1141 gccgctgatc gcgccgccgc cggcgccgcc gtccgatgcc gcatcgcccg gcgctccgcc
     1201 tcctgacccg gggcccgacc gcccctgacc tgattcgatg ttgttctcca gccccgatta
     1261 tccgctgttc ctgatcgcgg tgttcttcct gtacgcgctc gcgcgctggg gcgggccggc
     1321 gatggcgtgg gcgcggatct gcgtgatggt gctgctcggc gacgtggtgt tcgcgctggt
     1381 cgccaaggat cccgacgcgc tgtgggatcc gatcggcggc gtgctgctgc ggctcgcggc
     1441 cggggcgggg gcggtggtcg ggtccagtgc tccggcgggt tcgcccgcgg cgatcggcgt
     1501 gctcgcggtg cactgggcga tc
//
LOCUS       DASVOR010000543         2133 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_472676,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000543 DASVOR010000000
VERSION     DASVOR010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472676"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..1232)
                     /locus_tag="VF516_10820"
     CDS             complement(87..1232)
                     /locus_tag="VF516_10820"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEX8108212.1"
                     /translation="MRIWIWSMLVCGAMACAGCPKPGPQLVGGGSGANPGPGTNPNGG
                     QCANHQFLPSAQPELAITCPRKVCGLNGSWLGSGVPFRTLHLHGGANPEGLAILGFTD
                     RNGNAMTVDLTGDVLTGRPADGSPALTGAGLTGASLFLGPAGGKPTYQLTIDSVSFED
                     FWAQCPTCGGPVKQAPHYHFSATSLADRCQIALCDPALEHRPGVSILGTSVMFRGDYY
                     DDATYQVSDAPAPDRANPDGDLFNIACQGTAIYKLYMLRHTVASASAPAAITAPAQRT
                     ALLRLLTADYCGAGHPFTVNGVPIQLGTNPAMSPYRVTAASGYDLAASDAIDAQWTPA
                     GAACLGTPRLAHYMDPTTLMSKIHEVCTPPDCGATAPAGSYATSATR"
     gene            1578..2036
                     /locus_tag="VF516_10825"
     CDS             1578..2036
                     /locus_tag="VF516_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108213.1"
                     /translation="MHRTHKLISAAAAALIVGGLAAPALARRQNAFSGIAQQPSQATC
                     FSNGWGSIVNNCSATRTFCVPLVYDWSAFGNFANVTVTAFAPAGTLMSCQTTTTTKQG
                     VGWSWSGNVNVPMNGSWQDVVVNGGGIPDGGAAYTCCDIPSGGEISVVNY"
BASE COUNT          297 a          751 c          765 g          320 t
ORIGIN      
        1 aggcgcccgg acggcgcgcg gcgctcgcgg ccaccgcggc ggcgtggctg cgctcggtcg
       61 gcgggcgggc gagcgagatc gacgcgctag cgagtcgctg acgtggcgta ggagcccgcg
      121 ggggccgtcg cgccgcagtc cggcggcgtg cacacctcgt ggatcttcga catcagcgtg
      181 gtggggtcca tgtagtgcgc cagccgcggc gtccccaggc aggcggcgcc ggcgggtgtc
      241 cactgcgcat cgatcgcgtc gctggccgcc aggtcgtagc ccgacgccgc ggtcacccgg
      301 tacggcgaca tcgccgggtt ggtcccgagc tggatcggga cgccgttgac cgtgaacgga
      361 tgaccggcgc cgcagtagtc cgcggtgagc agccgcagca gcgccgtgcg ctgcgcgggc
      421 gcggtgatcg cggccggcgc gctcgccgac gccacggtgt ggcgcagcat gtagagcttg
      481 tagatcgccg tgccctggca cgcgatgttg aacagatcgc cgtcggggtt ggcgcgatcc
      541 ggcgcgggcg cgtcgctcac ctggtaggtc gcgtcgtcgt agtagtcgcc gcggaacatc
      601 accgacgtcc ccaggatgct gacgcccggg cggtgctcca gcgccggatc gcacagcgcg
      661 atctggcagc ggtcggccag gctcgtggcg gagaagtgat agtgcggcgc ctgcttgacc
      721 gggccgccgc aggtcgggca ctgggcccag aagtcctcga acgacacgct gtcgatggtc
      781 agctggtacg tcggcttgcc gccggccggg ccgaggaaca gcgacgcgcc ggtcaggccc
      841 gcgccggtca gcgccggcga gccgtcggcc ggccggccgg tcagcacgtc gccggtgaga
      901 tcgaccgtca tcgcgttgcc gttgcgatcg gtgaacccca ggatcgcgag gccctccggg
      961 ttggcaccgc cgtgcaggtg cagggtccgg aacggcacgc cggagccgag ccagctcccg
     1021 ttcagcccgc acaccttgcg cggacaggtg atcgcgagct cgggctgcgc cgacggcaag
     1081 aactgatggt tcgcgcactg cccgccgttc gggttcgtgc ccgggccggg gttcgccccc
     1141 gagccgccac cgaccagctg gggtccgggc ttcggacacc cggcgcaggc catcgcaccg
     1201 cacaccagca tcgaccagat ccagatccgc atctgctgac tctcctgggt tgctgtcctg
     1261 gatgcgcgag ccgggactgc gttcgccacg gtgcgccgtg cgaattcagc tcgcggcatc
     1321 atcgccggca tccattgcct cgggctcgtc cagcgcccgc gaagtcgttg ttcacggcgc
     1381 cagcctatca tggtgaatcg cgggcttgat ggatcgcgcc ggagtcgccg gagtcatgta
     1441 acgcgcggcg aggtccgacg actaccggga tgtatcgagt cgcgctgctc gtgcagggcg
     1501 ctcaccggcg acggcgtcgt gaggggcgac gtcgtgttcc ggcatcacgc acgtcttccc
     1561 cggaaaggtc acgtatcatg catcgcacac acaagctgat ctctgcagcc gccgccgcgc
     1621 tcatcgtcgg ggggctcgcc gcgcccgcgc tcgcccgccg gcagaacgcg ttctccggca
     1681 tcgcgcagca gccgagccag gcgacctgct tctccaacgg ctggggcagc atcgtcaaca
     1741 actgctccgc gacgcgcacg ttctgcgtcc cgctggtcta cgactggagc gcgttcggca
     1801 acttcgccaa cgtcacggtc accgcgttcg cgccggccgg cacgctgatg agctgccaga
     1861 ccacgacgac caccaagcag ggcgtcggct ggtcatggtc gggcaacgtc aacgtcccga
     1921 tgaacggctc gtggcaggac gtcgtcgtca acggcggtgg gatccccgac ggcggcgcgg
     1981 cgtacacctg ctgcgacatc ccgtccggcg gcgagatctc cgtcgtcaac tactgagcca
     2041 cgcaacccgg gggcgcggcc gcgccgcgtc ccccttccct ttcaggagca gccatgacga
     2101 tctggcgaac gtttctcatc gtgggcgcgg tcg
//
LOCUS       DASVOR010000544         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443238,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000544 DASVOR010000000
VERSION     DASVOR010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443238"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1517)
                     /locus_tag="VF516_10830"
     CDS             complement(<1..>1517)
                     /locus_tag="VF516_10830"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8108214.1"
                     /translation="AYARAFREDPAVEAAKQQLEALAALLDDGWTRLVGLFEAALTGG
                     RRANDLDPTLAHELATKVARSYEDRLGNSDKAVEYYKKALAIEPDDLAALAALEAIFT
                     RDDKFPELLDVYRRRVDIAEEPDERLEFLFRIAALHEEMLGNQDEAIATYIEILGQAS
                     DDMKALRALDRLYVARGAWRDLGDNISRQLTLVGMSESGPDDQVALLVRLAQLRETHL
                     GETAAAVETYRQVLELEDQNRDAIAALERLIGAAGGEHELTIASILEPIYRARADWKQ
                     QIGVYEIMARHSFDPVRKIELHHKIAELHEIGGDDAEAAFATFSRALREDPRNEVTHR
                     QLDRLARTLERWPESAALYDQVAGDAQEDDLRVALLFRRALIQEQELRDDRAAVATYE
                     RVLQAAPQAIEAVSAIQAIHERTGDWPRLVDALKRKAEIIPEVDERKALLYRSAQIEE
                     EVLSDPDAAIATFRQVLSIDDVDLVAMDALERLYVRLERWEPLKDVYAKKADLAE"
BASE COUNT          182 a          529 c          559 g          247 t
ORIGIN      
        1 ctcggcgagg tcggccttct tggcgtagac gtccttgagc ggctcccagc gctcgagccg
       61 gacgtacagc cgctccagcg cgtccatcgc gaccaggtcg acgtcgtcga tcgacagcac
      121 ctggcggaac gtcgcgatcg cggcgtcggg gtcggacagc acctcttcct cgatctgcgc
      181 cgagcggtag agcagcgcct tgcgctcgtc gacctcgggg atgatctcgg ccttgcgctt
      241 gagcgcgtcg accagcctgg gccagtcgcc ggtgcgctcg tggatcgcct ggatcgccga
      301 cactgcctcg atcgcctggg gcgcggcctg gagcacccgc tcgtaggtcg cgaccgcggc
      361 ccgatcgtcg cgcagctcct gttcctggat cagggcccgg cggaacagca gcgcgacccg
      421 gaggtcgtcc tcctgggcgt cgccggcgac ctgatcgtag agcgcggccg actcgggcca
      481 ccgctcgagc gtgcgggcga gccggtcgag ctggcggtgg gtgacctcgt tgcgcgggtc
      541 ctcgcgcagc gcgcgcgaga acgtggcgaa cgcggcctcg gcgtcgtcgc cgccgatctc
      601 gtggagctcg gcgatcttgt ggtgcagctc gatcttgcgg accggatcga acgagtgccg
      661 cgccatgatc tcgtacacgc cgatctgctg cttccagtcg gcgcgcgccc ggtagatcgg
      721 ctcgaggatg ctggcgatcg tcagctcgtg ctcgccgccg gcggcgccga tcaggcgctc
      781 gagcgcggcg atcgcgtcgc ggttctggtc ctcgagctcg agcacctggc ggtaggtctc
      841 gaccgccgcg gcggtctccc cgaggtgggt ctcgcgcagc tgggcgagcc ggaccagcag
      901 cgcgacctga tcgtcgggcc ccgactccga catcccgacc agggtcagct ggcggctgat
      961 gttgtcgccg aggtcgcgcc acgcgccgcg cgcgacgtac agccggtcga gcgcgcgcag
     1021 cgccttcatg tcgtcggacg cctggccgag gatctcgatg taggtcgcga tcgcctcgtc
     1081 ctggttgccc agcatctcct cgtggagcgc cgcgatccgg aacaggaact cgagccgctc
     1141 gtcgggctcc tccgcgatgt cgacccggcg ccggtagacg tcgagcagct cggggaactt
     1201 gtcgtcgcgg gtgaagatcg cctcgagcgc ggcgagcgcg gcgaggtcgt cgggctcgat
     1261 cgcgagcgcc ttcttgtagt actcgaccgc cttgtcgctg ttgccgagcc ggtcctcgta
     1321 ggaccgcgcg accttggtcg cgagctcgtg ggccagggtc gggtcgaggt cgttggcgcg
     1381 gcggccgccg gtcagcgcgg cctcgaacag cccgaccagc cgggtccagc cgtcgtcgag
     1441 cagcgcggcg agcgcctcga gctgctgctt ggcggcctcg acggcggggt cctcgcggaa
     1501 cgcgcgggcg taggcgt
//
LOCUS       DASVOR010000545         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443247,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000545 DASVOR010000000
VERSION     DASVOR010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443247"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..856
                     /locus_tag="VF516_10835"
     CDS             <1..856
                     /locus_tag="VF516_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="deoxyribodipyrimidine photolyase"
                     /protein_id="HEX8108215.1"
                     /translation="WKPAPAALLTGSPAALAAITIDHAVAPVGYRGGAVAAGAALDDF
                     IAHKLARYGAGHNHPDADAASGLSPYLHFGHVSAHEVAARVWTSAGWDPSRLADRPTG
                     RREGWWGLPAGAEAFLDQLVTWRELGYGFCFHRADFDRYASLPDWARRTLDDHAADPR
                     PELYSRRALEAANTADPVWNAAQRQLLVDGRIHNYLRMLWGKKILAWSRSPRAALATL
                     IELNNKYSVDGRDPNSYTGILWALGGFDRPWGPVRPIFGTVRYLSSAATVRKLRLRAY
                     LARWSGAR"
     gene            complement(864..1118)
                     /locus_tag="VF516_10840"
     CDS             complement(864..1118)
                     /locus_tag="VF516_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108216.1"
                     /translation="MTHYKAGGYTLCPSSPCLEYRSVAGSAEIPVCLRGTISGRTYVW
                     GIFIDGATSGTAADNTFNAAKAEPLREPIRSALAGWGACI"
     gene            1129..1383
                     /locus_tag="VF516_10845"
     CDS             1129..1383
                     /locus_tag="VF516_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108217.1"
                     /translation="MNWLISLAVRPNAGASASTIELTALWIPVKSPDHDALAQARALV
                     DLAHRVEADQDLDRARRLEDPGLLQRGLDAADPCQARLAR"
     gene            complement(1466..>1757)
                     /locus_tag="VF516_10850"
     CDS             complement(1466..>1757)
                     /locus_tag="VF516_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108218.1"
                     /translation="VVTASRDHSARVWDAATGNPVTPPLRHRGFVRAAVFSPDGTHIV
                     TKSIHKAVQLWNLRVDAGSLDDWRELARCCPFVLIDNVLADNPDPVRIRRSR"
BASE COUNT          247 a          648 c          613 g          249 t
ORIGIN      
        1 ctggaagccg gcgcccgcgg cgctcctgac cggcagcccg gccgcgctgg cggcgatcac
       61 gatcgaccac gccgtcgcgc cggtcggcta ccgcggcggg gcggtcgccg ccggcgccgc
      121 gctcgacgac ttcatcgcgc acaagctggc gcgctacgga gccggccaca accaccccga
      181 cgccgacgcc gcgagcgggc tgtcgccgta cctgcacttc ggccacgtct cggcccacga
      241 ggtcgccgcg cgggtgtgga cgtccgcggg ctgggatccg tcgcggctcg ccgaccgacc
      301 gacgggccgc cgcgagggct ggtgggggct gccggccggc gcggaggcgt tcctcgacca
      361 gctcgtcacc tggcgcgagc tcggctacgg gttctgcttc caccgcgccg acttcgaccg
      421 ctacgcgtcg ctgccggact gggcgcggcg gacgctcgac gaccacgccg ccgatccccg
      481 gcccgagctc tattcccgcc gcgccctgga ggcggcgaac accgccgatc cggtgtggaa
      541 cgccgcccag cgccagctgc tcgtcgacgg ccggatccac aactacctgc gcatgctgtg
      601 gggcaagaag atcctcgcgt ggtcgcggtc gccgcgcgca gcgctcgcca cgctgatcga
      661 gctcaacaac aagtacagcg tcgacggccg cgatccgaac tcgtacaccg gcatcctgtg
      721 ggcgctcggt gggttcgacc ggccgtgggg gccggtccgt ccgatcttcg gcaccgtccg
      781 ctacctgtcg tcggcggcga ccgtgcgcaa gctgcgcctg cgcgcgtacc tcgcgcgctg
      841 gagcggcgcg cgctgaccgg tgtttagatg caggcgcccc agcccgccag cgccgagcgg
      901 atcggctcgc gcagcggctc ggccttcgcg gcgttgaacg tgttgtcggc cgcggtgcca
      961 ctggtcgcgc cgtcgatgaa gatgccccac acgtaggtgc ggccactgat cgtgccgcgc
     1021 aggcacaccg ggatctcggc cgagccggcg accgagcggt actccaggca cgggctcgac
     1081 gggcacagcg tgtagccgcc cgccttgtag tgggtcaaga gccggctctt gaactggctg
     1141 atctcgctcg cagtcaggcc gaacgccggg gcctcggcgt cgacgatcga gttgaccgcg
     1201 ctctggatcc cggtgaagtc accagaccac gacgctctgg ctcaggctcg cgccctggtc
     1261 gacctggcgc atcgcgtgga ggccgatcag gatcttgatc gagctcgccg gctcgaagat
     1321 ccgggtctcc tgcagcgcgg cctggacgcc gccgacccat gtcaggcccg tttggccaga
     1381 tagagtcagc cccgagggga tgtgacaggc gatccgagat ccgctaaccc acggatctcg
     1441 cacgggatgg caagcgctgc cgagcctacc gcgagcggcg aatgcgcacc gggtccgggt
     1501 tatcggcgag cacgttgtcg atcaggacga acgggcagca tcgagcgagc tcgcgccagt
     1561 cgtccagcga gcccgcgtcg actcgcaggt tccagagctg cacggccttg tggatactct
     1621 tcgtcacgat atgggtccca tccggactga acaccgccgc acgaacgaag ccacggtgcc
     1681 gcagcggcgg cgtcaccggg ttcccggtcg ccgcgtccca gacccgcgcc gagtggtcgc
     1741 ggctcgcggt caccacc
//
LOCUS       DASVOR010000546         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_326484,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000546 DASVOR010000000
VERSION     DASVOR010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326484"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(700..1149)
                     /locus_tag="VF516_10855"
     CDS             complement(700..1149)
                     /locus_tag="VF516_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8108219.1"
                     /translation="MRDPLQLDDQLCFALYSASRAVIRAYAPLLQPLGLTYPQYLVLL
                     VLWERDGASVKQLGERLALDSGTLTPLLQRLEHQGLVERRRGDDDERVVRIHLTAAGR
                     ALRGKARKIPAELACRAGYDLASDGSVRDLVRLRDELTALARRLRGS"
     gene            complement(1157..1600)
                     /locus_tag="VF516_10860"
     CDS             complement(1157..1600)
                     /locus_tag="VF516_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEX8108220.1"
                     /translation="MSNTPTVLDKRLYTAVATATGGRDGRAKSDDGVLDVALVPPKSL
                     GGGGATGTNPEQLFAAGYAACFGSAIGAVARGKKVTTGPVAVTARVALGTAGQAYGLA
                     VELEVSLPELPRDQAEALVTAAHQVCPYSNATRGNIVVDVKLAGQ"
     gene            complement(1733..2608)
                     /gene="panB"
                     /locus_tag="VF516_10865"
     CDS             complement(1733..2608)
                     /gene="panB"
                     /locus_tag="VF516_10865"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEX8108221.1"
                     /translation="MKTTKVTIHTLRQMKARGEKIAMLTAYDATFARLFDEAGADVLL
                     VGDSLGMVVQGHDTTLPVTLDEIAYHCRAVARGARRSHVVGDMPFMSYQASLEQGVTN
                     AGRLMKEGGCHSVKLEGGAVHAELVTRLVASGIPVMGHIGLTPQSFHQLGGFKVQGRD
                     PGGRERLLDDARTLEQAGVYSIVLEAIPADIAYDITAAVSVPTIGIGAGAGCDGQVLV
                     CYDALGMDETFKPRFVRRYATLGQTVKDAIGHYITDVRGGGFPSDAESFTLAESKAAP
                     GALEPAPYSSAGKHL"
BASE COUNT          391 a          939 c          986 g          391 t
ORIGIN      
        1 atcaggtgcg ctgtcgccgt gtccggtcga gatggggaga gggccccgga gaccgctgga
       61 cgctggctcg ctggatgacc tgcgggcgga ggtgtaccgg ctgacctcgg cgcgcagttc
      121 accagatccg gaccgaacaa atacatccgg aacggagcgt cggcgcgggg cgatgcactg
      181 tgagtggggc gtccaggtca acctggtgag ccgcggcgcg cctgtcacag ggccgtccac
      241 gcggtctccc ccgtctgcgc cgacgatggt ggtcactcgt cggccgtttc accggatccg
      301 tcgtctgcac gaagaccgcg cggcagcaca cccatcgctt cgcatcgtca acgggcaggt
      361 catcgcgctc acggcacagc tggaggcacg ccggagcgca gcgggtactg agccgaggta
      421 gcgcactggg cagtccagcc gagcagccga cgagggacca ccatcctcat cacaggcggc
      481 cgggccacgg cccgggagac cgcgcggacg gccctgtgac tggcccgttg aagcggacca
      541 ggccaaccag gacggcccac tcacagtgca tcgccccgcg cgaacgctcc tgttccggat
      601 ctcattggat cgttcccgga tccccggaaa gggcacgctc gacggcttcg ggtgcctccc
      661 tcctcgccga gcatcgcagc tgacgcctgg gcgccagggt cacgatccgc gcagccggcg
      721 ggccagcgcg gtcagctcgt cgcgcagccg cacgagatcg cgcaccgagc catcgctcgc
      781 gaggtcgtag ccggcgcggc acgcgagctc cgccgggatc ttgcgggcct tgccgcgtag
      841 cgcgcgcccc gcggccgtga gatggatgcg gaccacccgc tcgtcgtcgt cgccgcgccg
      901 gcgctcgacc aggccctggt gctcgaggcg ctggagcagc ggggtcagcg tgccggagtc
      961 gagcgcgagc cgctcgccga gctgcttgac cgacgcgccg tcgcgctccc acagcacgag
     1021 cagcacgagg tactgcggat aggtcagccc gagcggctgg agcagcggcg cgtaggcgcg
     1081 gatcaccgcg cgcgatgcgc tgtacagcgc gaagcacagc tgatcgtcga gctgcagcgg
     1141 atcgcgcacc gccatgtcac tgcccggcga gcttgacgtc gaccacgatg ttgccgcgcg
     1201 tcgcgttgga gtacgggcag acctggtggg cggcggtgac cagcgcctcg gcctgatcgc
     1261 gcggcagctc gggcaggctg acctcgagct cgaccgcgag gccgtaggcc tggccggcgg
     1321 tgccgagcgc aaccctggcg gtcaccgcga ccgggccggt ggtgaccttc ttgccccgcg
     1381 cgaccgcgcc gatcgcgctg ccgaagcacg ccgcgtagcc ggcggcgaac agctgctcgg
     1441 ggttggtgcc ggtcgcgccg ccgccgccga gcgacttcgg cgggaccagc gcgacgtcga
     1501 gcacgccgtc gtcggacttg gcgcgcccgt cgcggcctcc ggtggcggtg gcaacggcgg
     1561 tgtaaagacg cttgtcgagg accgtggggg tgttggacat ggcgatatcg atagccacca
     1621 attaaattgt gcgcaatcga attgcgcgaa attcgtggca cggcgcgtgc agccggcgtc
     1681 cgccggcgcg cgctccggat ccgcccgctg gatccgaccg tgaggcgagc ggctacaggt
     1741 gcttgccggc cgacgagtac ggcgccggct ccagcgcgcc cggggcggcc ttgctctcgg
     1801 cgagcgtgaa cgactcggcg tcggacggga agccgccgcc gcgcacgtcg gtgatgtagt
     1861 ggccgatcgc gtccttcacg gtctgaccga gcgtggcgta gcgccggacg aaccggggct
     1921 tgaacgtctc gtccatgccg agcgcgtcgt agcacaccag cacctggccg tcgcagcccg
     1981 cgccggcgcc gatgccgatc gtcgggaccg acaccgccgc ggtgatgtcg tacgcgatgt
     2041 cggcggggat cgcctcgaga acgatcgagt acacgccggc ctgctccagg gtgcgggcgt
     2101 cgtcgagcag gcgctcgcgt ccgccgggat cgcggccctg gaccttgaag ccgccgagct
     2161 ggtggaagct ctgcggggtc aggccgatgt gtcccatcac cgggatgccg gacgcgacca
     2221 gccgggtgac gagctcggcg tggaccgcgc cgccctcgag cttgaccgag tggcagccgc
     2281 cctccttcat caggcggccg gcgttggtga cgccctgctc gagcgatgcc tggtacgaca
     2341 tgaacggcat gtcaccgacg acgtgcgacc gccgcgcgcc gcgcgccacc gcgcggcagt
     2401 ggtaggcgat ctcgtcgagg gtgaccggca gggtcgtgtc gtggccctgg acgaccatgc
     2461 cgagcgagtc gccgaccagg aggacgtcgg cgccggcctc gtcgaacagc cgggcgaacg
     2521 tcgcgtcgta ggcggtgagc atcgcaatct tctcgccccg cgctttcatc tgccggaggg
     2581 tgtggatggt gaccttcgtg gtcttcattc cgcccttcct ttctgtgggg tatgcccggg
     2641 atcagacccg acaggagtcg cgcgccgcac catgcggcta cgtgcacctt ggctacctgc
     2701 tgaatgg
//
LOCUS       DASVOR010000547         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_5175,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000547 DASVOR010000000
VERSION     DASVOR010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5175"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF516_10870"
     CDS             <1..502
                     /locus_tag="VF516_10870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HEX8108222.1"
                     /translation="QFGKEWDECDSMDQKRHWDRRTGLGRKRLFEVRKLYNDITFIDE
                     FFTLEFCIEQKFYSFGFSERSGNWEIMSREFKKVKDQLLRMLTNRGQPVILVEDGNFE
                     NRSELLLRHIHEGIDLDAGQARDTLRNTSKLWTRPVSLLTKVEGKGKLLRCEDGNLTE
                     RSAEYH"
     gene            524..1432
                     /locus_tag="VF516_10875"
     CDS             524..1432
                     /locus_tag="VF516_10875"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX8108223.1"
                     /translation="MRLWAWGQSDPGRKRERNEDSYLVDPETGVMAVADGMGGHQGGA
                     TASRMAVEYLGRELAEVRGNFDAALLEQIQGEMHVRTTEEMPVVSDLAEASTPPSGSK
                     PVVGGPVRGSDPTLPPGAVPAFSPALEVMRGVVRRASSAIFEASWARPELRGMGTTLT
                     AMLVHGGRAHVVHAGDSRCYVFRDGQLRQLTEDHSWIAEQLRAGSISEAEARSSKFRH
                     VITKSIGFERDIEADTKSVPVSAGDCFLLCSDGMTNYVEHGELERIVAMTWYRRLPAT
                     LVELANSRGGDDNITVVVGLVANAPP"
     gene            complement(1580..>1782)
                     /locus_tag="VF516_10880"
     CDS             complement(1580..>1782)
                     /locus_tag="VF516_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108224.1"
                     /translation="GKHAEIATWPDHADAAHRIAATRLVLDFFRQQLGPPVRPQVMPV
                     AKDDDDEEEERERAKRGSEAIK"
BASE COUNT          290 a          602 c          633 g          257 t
ORIGIN      
        1 ccagttcggc aaggagtggg acgagtgcga ctcgatggac cagaagcggc actgggatcg
       61 ccgcaccggc ctggggcgca agcggctgtt cgaggtccgc aagctctaca acgacatcac
      121 gttcatcgac gagttcttca ccctggagtt ctgcatcgag cagaagttct actcgttcgg
      181 gttctccgag cgcagcggca actgggagat catgagccgc gagttcaaga aggtgaagga
      241 ccagctgctg cggatgctga ccaaccgcgg ccagccggtg atcctggtcg aggacggcaa
      301 cttcgagaac cgcagcgagc tgctgctccg ccacatccac gagggcatcg acctcgacgc
      361 cggccaggcc cgcgatacct tgcgtaacac ctcgaaacta tggacccgtc cggtcagcct
      421 gctgacgaaa gtcgagggca aagggaagct tttgcgctgc gaggacggaa atctgacaga
      481 acggagcgcg gagtatcact agtatgagcg agcggtgggc acgatgaggt tgtgggcgtg
      541 ggggcagagc gatccgggtc gcaagcgcga gcgcaacgag gacagctacc tggtcgatcc
      601 cgagaccggg gtgatggcgg tcgccgacgg gatgggcggc caccagggtg gcgccaccgc
      661 gagccggatg gccgtcgagt acctcggccg cgagctcgcc gaggtccgcg gcaacttcga
      721 cgccgcgctg ctcgagcaga tccagggcga gatgcacgtg cgcaccaccg aggagatgcc
      781 ggtggtcagc gacctcgcgg aggcgtcgac gccgccgtcg gggtccaagc cggtcgtcgg
      841 aggcccggtg cgcggcagcg accccacgct gccgcccggc gcggtgccgg cgttctcgcc
      901 ggcgctggag gtgatgcgcg gcgtggtccg gcgggcgagc tcggcgatct tcgaggcgtc
      961 gtgggcccgg cccgagctgc gcggcatggg cacgacgctg accgcgatgc tggtccacgg
     1021 cggccgcgcc cacgtggtcc acgccggcga ctcccggtgc tacgtgttcc gcgacggcca
     1081 gctccgccag ctgaccgagg atcactcgtg gatcgccgag cagctgcgcg ccggctcgat
     1141 cagcgaggcc gaggccaggt cgagcaagtt ccgccacgtg atcaccaagt cgatcggctt
     1201 cgagcgcgac atcgaggccg acaccaagag tgtgccggtc tcggccggcg actgcttcct
     1261 gctgtgctcc gacggcatga ccaactacgt cgagcacggc gagctcgagc ggatcgtcgc
     1321 gatgacctgg taccgccggc tgccggcgac cctggtcgag ctcgccaaca gccgcggcgg
     1381 cgacgacaac atcacggtcg tcgtcggcct ggtcgccaac gccccgccgt agcggctagc
     1441 cccggcggcg cgccggacgg accagcgcga ccagcgcgac cagcgcgagc cccggccatg
     1501 gcccggcgct cgcgcccgcg ctgcaccagt atctatcaca agctggggat ctcgtcctgc
     1561 cgcgagctca tggcgcagct cacttgatcg cctcgctgcc acgcttggcg cgctcgcgct
     1621 cttcttcctc gtcgtcatcg tcctttgcga ccggcatgac ctgcggccgc accggcgggc
     1681 cgagctgctg gcggaagaag tcgagcacga gccgggtcgc cgcgatccgg tgggcagcgt
     1741 cggcgtggtc cggccacgtc gcaatctcgg cgtgcttgcc gg
//
LOCUS       DASVOR010000548         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_472767,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000548 DASVOR010000000
VERSION     DASVOR010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472767"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VF516_10885"
     CDS             complement(<1..1013)
                     /locus_tag="VF516_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108225.1"
                     /translation="MRTSTFLIAILAVAAVTGHARPAAADLVHYHVLPETTDPGIRAA
                     DAKGRKAEHQIYMPAKGEGNGRLLVYFPGSGGEPEGATAIYETAARRGYHVIGLVYVN
                     DHSIDEMCKQDVSCPGKVLHENLDGKDRTPVLQVDRANSIENRLVKLLSWLEHNHPEG
                     KWGRYLTAKHNLRWRLIAAMGHSQGGATAAYIGKVRKLGRVAMLSAPSAGFVDATGKV
                     HSATWVKGSFRTDHQRYFLFASTKDPRYPRIEADSAAIGVPGPLTSVDGAKPPYGRSH
                     RLTTSVDAKNPHNSVLEAAFAPVWRTLIGGKVRRGPDRIAKAGPHGKPAGQAPAGQAP
                     AGPA"
     gene            1381..>2976
                     /locus_tag="VF516_10890"
     CDS             1381..>2976
                     /locus_tag="VF516_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108226.1"
                     /translation="MGVVVALGSALPLANLAFGEGAPHNGLSSLAFRFNALTTNRRAL
                     HALSGNALDASLFSADGGYIARQMRDPHARAMLTEMVACALNPGTGVSYDDPERGIHW
                     SGKGELGLCPAWPGGEAQRPSCQELVSACIMARVNAMDRSIPISLRSDPGRVPSSSPL
                     ASLDTGIPVNVRFREGMQNTDPAVGALIEGFTGPLCKAGSECDWRPAYVGTCAPGESV
                     QLVIADPDRCKRSTLRVCSGISGCVKDQASWKPYARFLAEARGACPPSASAGPPLHFR
                     CPMGAAVKGYYSVMVLPAPPDAPSSSPTPVTLTASGAPPVARIAGAGTYPAPEADVFS
                     FLEGAFYGNLFKPERLTWSCELDDAGRWSCSGGAVTASDLASCGSGRVGAVAGHRCSL
                     PVPYMDMYACYSYAQQVEDQEADALGAALLNSRICDLPSAAAKCFPHPLRRCHYKDPD
                     VNRTRGAHCDWIGNDGAYGHCPGQDGDGGMYHNIMTTYLNEPNDLIGDVELAARLRGS
                     LPPVTRAPAGVPPVPVRRCGCASD"
BASE COUNT          419 a          978 c         1085 g          494 t
ORIGIN      
        1 gccgggcctg cgggcgcctg gcctgcgggt gcctggcctg ctggcttgcc gtgcggcccg
       61 gccttggcga tgcggtccgg cccgcggcgc accttgccgc cgatcagggt gcgccacacc
      121 ggagcgaacg cggcctcgag cacggagttg tgcgggttct tcgcgtcgac cgaggtggtc
      181 aggcggtggg agcgaccgta gggcggcttg gcgccgtcga cggaggtcag cgggccgggc
      241 acgccgatcg cggccgagtc ggcctcgatc cgcgggtagc gcgggtcctt ggtgctggcg
      301 aacaggaagt agcgctggtg atcggtgcgg aacgagccct tcacccaggt cgcgctgtgg
      361 accttgccgg tcgcatcgac gaagcccgcc gacggcgccg acagcatcgc gacgcggccg
      421 agcttgcgca ccttgccgat gtacgcggcg gtcgcgccgc cctgcgaatg ccccatcgcc
      481 gcgatcaggc gccagcgcag gttgtgcttt gcggtgaggt agcggcccca cttgccctcg
      541 gggtggttgt gctcgagcca ggacagcagc ttgaccagcc ggttctcgat cgagttggcg
      601 cggtcgacct gcagcaccgg cgtgcggtcc ttgccgtcga ggttctcgtg cagcaccttg
      661 ccggggcaag acacgtcctg cttgcacatc tcgtcgatcg agtgatcgtt gacgtacacg
      721 aggccgatga cgtgatatcc gcgccgcgcg gccgtctcgt agatcgccgt cgcgccctcg
      781 ggttcaccgc cgctgcccgg gaagtacacg agcaagcggc cgttgccttc gcccttggcg
      841 ggcatgtaga tctggtgctc cgcctttcgt cccttcgcat ccgcggcgcg aatgccggga
      901 tcggtggtct cggggagcac gtggtagtgg acgaggtcgg cggcggccgg ccgggcatgt
      961 ccggtgaccg ccgcgaccgc caggatcgcg atgaggaatg tggatgtacg catgcgacgg
     1021 gcgtcagcaa gcgatgagcc acgcccggtc cggcacgatt cctgtaggtt ttgccggcaa
     1081 gtcttgccgg tccacgccgg gttcacgctg gagcgatgcc gcgcgggcga tcgatgatcg
     1141 tcgtgccggt gctcgcaggc ggcgtggccg cgagcgagtg cgcgaatcgc gcaggcggcg
     1201 ccgagggact tgcgcggatc gcgcaaggcg ggcgacattc ctccgagggt gccgagcccc
     1261 gcatccagcc tgtgcggcgg tacgagtgtt gcctctctgc agctcgctcc ggcggccaat
     1321 gccgccggat agaacggtaa ccaagggaaa gacgtgatgt ggaatcgtac gaactgcatc
     1381 gtgggggtgg tggtcgcgct ggggagtgcg ttgccgctgg caaacctcgc cttcggggaa
     1441 ggcgctcctc ataacggact gtcgagcctg gcgttccggt tcaacgcgct caccaccaac
     1501 cggcgcgcct tgcacgcgct atcgggcaac gccctggatg cgtcgttgtt ctcggcggac
     1561 ggcgggtaca tcgcccgcca gatgcgggat ccgcatgccc gggcgatgct gacggagatg
     1621 gtagcgtgcg ccctgaaccc cgggaccggg gtgtcctacg acgatccgga gcgaggcatc
     1681 cactggtcgg ggaagggaga gctcgggctg tgtccggcat ggccgggggg agaggcgcag
     1741 cgcccgtcct gccaggagct ggtctcggcc tgcatcatgg cacgcgtgaa cgccatggac
     1801 cgctccatcc cgatctcgtt gcggagcgat cccggacggg tgccgtcgtc ctcgccgctg
     1861 gcgtctctcg acaccgggat ccccgtcaac gtcaggttca gagagggtat gcagaatacc
     1921 gatccggcgg tgggggctct gatcgagggt ttcacaggac cgctgtgcaa ggcgggcagc
     1981 gagtgcgact ggagacctgc ctatgtcggc acgtgtgcac cgggggagtc ggtccagctc
     2041 gtgatcgcgg atccggaccg ctgcaagcgg tccacgctgc gggtgtgcag cgggatctcc
     2101 ggttgtgtca aggaccaggc gtcgtggaag ccctatgcga gattcctcgc cgaggcccga
     2161 ggcgcctgcc cgccgagcgc gtccgctggc ccgccgctgc acttccgatg tcccatgggt
     2221 gctgcggtca agggctacta cagtgtcatg gtcctgccgg cgccgcccga tgctccgtcg
     2281 tccagcccga cccccgtcac gctgactgcc tcgggtgcgc cgcccgtcgc ccggatcgcc
     2341 ggcgccggca cgtatccggc gccggaggcc gacgtgttca gcttcctcga aggtgcgttc
     2401 tacggcaacc tgttcaaacc ggagcggctg acgtggagct gcgagctgga cgatgccggg
     2461 cggtggagct gctcgggcgg cgccgtcacc gcgtccgacc tcgcgagctg cggctcgggc
     2521 cgggtgggtg cggtcgcggg ccaccgatgc tcgctgccgg tcccctacat ggacatgtat
     2581 gcctgctact cgtatgcgca gcaggtcgaa gatcaagagg ccgatgcgct gggtgccgcg
     2641 ctgctgaact ctcggatctg cgatctgccg agtgcagctg cgaagtgctt cccgcatccg
     2701 ctccggcgct gtcactacaa ggatccagac gtcaaccgca cccgaggcgc ccactgcgac
     2761 tggatcggca atgacggcgc ctatggccac tgcccgggac aggacggcga cggcgggatg
     2821 taccacaaca tcatgacgac ctacctcaac gagccaaacg atctgatcgg ggacgtcgag
     2881 ctggcggctc ggctgcgcgg gtcgctccct ccggtcacgc gagctccggc gggagtgccg
     2941 ccggtgccgg tccggcgctg cggctgcgcg agcgac
//
LOCUS       DASVOR010000549         5591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413645,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000549 DASVOR010000000
VERSION     DASVOR010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413645"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VF516_10895"
     CDS             complement(<1..468)
                     /locus_tag="VF516_10895"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108227.1"
                     /translation="MATIIGGIGTSHVPTIGMAFDKAKQADPSWAPLFAGYEPVARWL
                     ADRRPDVLVFCFNDHATTFFFDRYPTFALGVGDAYPIADEGTGPRPIPRLRGHAQLSR
                     HIACSLVADEFDLTIFQDLPLDHGLHSPLTMMWPSQPDWPGQIVPLVVNVLQHP"
     gene            610..1149
                     /locus_tag="VF516_10900"
     CDS             610..1149
                     /locus_tag="VF516_10900"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011551675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8108228.1"
                     /translation="MLTIGDPFPAFTAKAVVSLDRGHEFETLSDQSFAGKWKVYFFWP
                     KDFTFVCPTEIAAFGKLEREFAERDAQVLGVSTDSEYVHLAWRQQHPDLRDLPFPMIA
                     DLKRELSQALGVIDKREGVALRATFIVDPQGVIRFASVNDLSVGRNPGEVLRVLDALQ
                     TDALCPCNWQKGEATLSAA"
     gene            1184..1795
                     /locus_tag="VF516_10905"
     CDS             1184..1795
                     /locus_tag="VF516_10905"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8108229.1"
                     /translation="MTTSNLSPIPAPASAPASAVTAPIAAIERIKRRIPDHARDLRIN
                     LGVIAAATALSPQQAWGTAVAAAVTSRNPEVLAAIEEAAAPHLSPEALAAAPAAASIM
                     AMNNVYYRFVHLMGDDSEYGQMPARLRMQVIAKPGVAPLDFELWCLAASAIAGCEKCV
                     RAHEASVRERGGTAEHVHDAVRIAAVVHAVAVTLNAAPDPPGR"
     gene            1860..3380
                     /locus_tag="VF516_10910"
     CDS             1860..3380
                     /locus_tag="VF516_10910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108230.1"
                     /translation="MKFYYASPEPDRLPAGLRAIAKTGVPEEHMHLVVATFLGLIMKA
                     HPERIASWFTELGDLEGIWRTTLHLAVWSSGTPEGRACLLQAGVTPDQLPSAIDALRH
                     PIDQPVVLDMLWAHYSATGDAAAVRRIISVLEHLGDQGAAARLPETQQTPEDRARAMR
                     DMLFQAAQGSLLNRMREHAPLFALCERIFEDPDLGPNERVCLAMIFETVDPLAWHVQI
                     DPVKNKARVDRKPRNMDASVSGDLEAFRVVMTGYYRKPDGQLAAGALRHWLRLLARSD
                     EEQRACMEGRLYVLARISQVSEAARDAFEPILRAFKGPRADPHAELVQGLLRVARDPT
                     FPNAVQLPLKDAGSLDLLWGEFFVTGQREPIERIFSTLDWDDRVRQRLEAWLRTTSLF
                     GGSKRRAAASTLASAGLVVDLERKVIVSTGDLDCLCFSIAKQRVPIFKMLPFELTPAE
                     VTALATKAQALWSLCLNARDHEKVAEVCRIEAQRPGGPARLRATEPAGDDAPPFAL"
     gene            3418..4029
                     /locus_tag="VF516_10915"
     CDS             3418..4029
                     /locus_tag="VF516_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8108231.1"
                     /translation="MVDPVAAPRPRARGREKVAWQAPQSTLGGCRGRWRHKTSPVRGE
                     RVHEPVRHLARGTDDWLRRPLVRYQLVRGDKELCIVATDNDASAGALLWREVGTDTWH
                     ERSLAPLEFQLLRALCVRAHEEASWPSTIRGCVPTQQLVNDLPFQSKYANQENVRQVV
                     LRLRGVLAEIGVASLLAVVPGRGYYLASPVTVGTTSLAEQLRR"
     gene            5332..>5591
                     /locus_tag="VF516_10920"
     CDS             5332..>5591
                     /locus_tag="VF516_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108232.1"
                     /translation="MLQRIVDSGRVPEPMLRAGIRVVCALRLYQERRRPPGYRPALVA
                     ELRRSEIAIAPDAANAQHYEVPAAFFARVLGPHLKYSCAYWPA"
BASE COUNT          853 a         1959 c         1920 g          859 t
ORIGIN      
        1 cgggtgctgc agcacgttga ccaccagcgg cacgatctgc cccggccagt cgggctggct
       61 cggccacatc atcgtcagcg gcgagtgcag gccgtggtcg agcggcaggt cctggaagat
      121 cgtcagatcg aactcgtcgg cgaccagcga gcacgcgatg tggcgcgaca gctgtgcgtg
      181 gccacgcagc cgtgggatcg gccggggccc tgtgccctcg tcggcgatcg ggtaggcgtc
      241 gccgacgccc agcgcgaacg tcgggtagcg gtcgaagaag aacgtggtcg cgtggtcgtt
      301 gaagcagaac accagcacgt cgggccgccg gtccgcgagc cagcgcgcga ccggctcgta
      361 gccggcgaac agcggcgccc agctgggatc cgcctgcttg gccttgtcga acgccatgcc
      421 gatcgtgggc acgtgcgagg tgccgatgcc gccgatgatg gtcgccaccg gccgactgta
      481 ccctgccccc gccggtcgat gacgccgcgt gcgagatcgt cctgaaagcc gccatctatc
      541 ggctccatag agtcgcgcga ttggcgttat caggcgtgcg gcggcatggt gctcgcagga
      601 gacatcacca tgctcaccat cggagatccc ttccccgcct tcaccgcgaa ggccgtggtc
      661 agcctggatc gcggccacga gttcgagacc ctgtcggatc agagcttcgc gggcaagtgg
      721 aaggtctact tcttctggcc caaggacttc acgttcgtgt gcccgaccga gatcgccgcg
      781 ttcggcaagc tcgagcgcga gttcgccgag cgcgacgccc aggtgctcgg cgtgtcgacc
      841 gacagcgagt acgtccatct cgcatggcgc cagcagcacc ccgatctacg ggatctgccg
      901 ttcccgatga tcgccgacct caagcgcgag ctgtcgcagg cgctcggcgt gatcgacaag
      961 cgcgagggcg tggcgctgcg cgcgacgttc atcgtcgacc cccagggcgt gatccggttc
     1021 gcctcggtca acgacctgtc ggtcggccgc aacccgggcg aggtgctgcg cgtgctcgac
     1081 gcgctgcaga ccgacgcgct gtgcccgtgc aactggcaga agggcgaggc gacgctgtcg
     1141 gcggcgtgac ccgcgacccg agggagccag tgaggatcca tcgatgacca cgtcgaacct
     1201 gtcaccgatc ccggcgcccg cgtcggcgcc cgcgtcggcg gtcaccgcgc cgattgccgc
     1261 gatcgaacgg atcaagcgcc ggatccccga tcacgcccgc gacctgcgga tcaacctcgg
     1321 cgtgatcgcc gcggcgaccg cgctgtcgcc acagcaagcc tggggcaccg cagtcgcggc
     1381 cgcggtcacc tcgcgcaacc ccgaggtgct cgccgcgatc gaggaggcgg cggcgcccca
     1441 cctgtcgccg gaggcgctgg ccgccgcgcc cgccgccgcg tcgatcatgg cgatgaacaa
     1501 cgtctactac cgcttcgtgc acctgatggg cgacgactcg gagtacggcc agatgccggc
     1561 gcggctgcgc atgcaggtca tcgccaagcc cggcgtcgcc ccgctcgact tcgagctgtg
     1621 gtgtctcgcg gcgtcggcga tcgccggctg cgagaagtgc gtgcgcgccc atgaggcgtc
     1681 ggtgcgcgag cgcgggggga cggcggagca cgtccacgac gcggtgcgga tcgccgcggt
     1741 cgtgcacgcg gtggcggtga cactcaacgc cgcgcccgac ccgccgggtc gctgacctgc
     1801 atcgtccggc tcactggctc ggggatctgg catcatgtcc cggcgtgctg acggactgga
     1861 tgaagttcta ctacgcgagt cccgagcctg atcggctgcc cgccgggctg cgcgcgatcg
     1921 cgaagacagg cgtacccgag gagcacatgc accttgtcgt cgcgacgttc ctcgggctca
     1981 tcatgaaggc tcatcccgaa cgcattgcca gctggttcac cgagctcggc gatctcgagg
     2041 gcatctggcg cacaaccctg catctggccg tgtggagctc gggtacgccc gagggacgcg
     2101 cgtgcctcct gcaggccggg gtcaccccag atcagctgcc gtcggcgatc gatgccttgc
     2161 gtcatccgat cgaccagcct gtggtgctgg acatgttgtg ggcgcactac tccgcgaccg
     2221 gcgacgcggc cgccgtgcgg cggatcatct cggtgctcga gcatctcggc gatcaaggcg
     2281 ctgctgcacg gcttccggag acccaacaga cgccggagga ccgtgcgcgc gcgatgcgag
     2341 acatgctgtt tcaagcagca caagggtctc tcctgaaccg gatgcgagag cacgcgccgc
     2401 tgttcgcgct atgtgaacgg atcttcgagg atccggatct cggaccgaac gagcgcgtct
     2461 gcctcgcgat gatcttcgag acggtcgatc cactggcgtg gcacgtgcag atcgatccgg
     2521 tcaagaacaa ggctcgcgtt gatcggaagc ctcgcaacat ggacgcatcg gtcagcggcg
     2581 atctcgaggc atttcgcgtc gtgatgaccg gatattaccg caaaccggac gggcagctcg
     2641 ccgcaggggc gctgcgacac tggttgcgct tgctggcaag gtcggacgaa gagcaaaggg
     2701 cttgcatgga ggggcggctc tacgtgttgg cgcgaatcag tcaggtgtcg gaggcggcac
     2761 gtgatgcctt cgaaccgatc ctgcgcgcct tcaaaggtcc gcgcgctgat ccgcacgccg
     2821 agctcgtcca ggggctgctc cgggttgccc gggatcccac gtttcccaat gcggtccagt
     2881 tgcccctcaa ggacgctggg tccctcgatc tgttgtgggg cgaattcttc gtgaccgggc
     2941 agagagagcc gatcgagcgc atcttcagca cgctcgactg ggacgatcgc gtgaggcagc
     3001 ggctcgaggc gtggctgcgc acgacgtcgc tgttcggtgg atcgaagcgg cgtgcggcag
     3061 cgtcgacgct cgcctcggcc gggctcgtcg tggatctcga gcgcaaggtc atcgtgtcaa
     3121 cgggcgacct cgactgcctg tgcttctcga tcgccaagca gcgcgtgccg atcttcaaga
     3181 tgctgccgtt cgagctgact cccgccgagg tgacggctct cgcaacgaag gctcaggcgc
     3241 tgtggtcgct ctgtctgaac gcgcgggacc acgagaaggt cgccgaggtc tgccgcatcg
     3301 aagcgcagcg ccccggaggg ccggcccggc tccgtgcgac cgagcctgcc ggcgacgacg
     3361 ctccaccatt cgccttgtag cgatggctcg ccgatctcgc ccctcgacgc cgggtctgtg
     3421 gtcgatccgg tcgcggcgcc cagaccacga gccaggggcc gcgaaaaggt cgcttggcag
     3481 gcgccgcaat caacgctcgg gggttgccgc ggccggtgga ggcacaaaac ttcgcccgta
     3541 cgtggggagc gcgtccacga gccggtccgg catctggcga ggggcaccga cgattggctc
     3601 cgtcgcccgc tggtccgcta ccagctggtc cgtggcgaca aggagctgtg catcgtcgcc
     3661 accgacaacg acgcctcggc cggggcgctg ctgtggcgcg aggtcggcac cgacacctgg
     3721 cacgagcgca gcctcgcgcc gctcgagttc cagctgttgc gcgcgctgtg cgtcagggct
     3781 cacgaggaag cgtcgtggcc gtcgacgatc cgcggctgcg tgccgaccca gcaactggtc
     3841 aacgacctgc cgttccagtc caagtacgcc aaccaggaga acgtgcgcca ggtcgtgctg
     3901 cggctgcgcg gcgtgctcgc cgagatcggc gtcgcgagcc tgctcgcggt cgttcccggc
     3961 cgtggctact acctggcgag cccggtcacc gtcggcacca ccagcctggc cgagcaactc
     4021 cgccgctgac gcgcccggat cctggtgcga agcgcgtcgt gatcgacccg gctgctgggg
     4081 ggcgagtacg gatcgtcaac atcgcgcccc ctcggcgacc ggggccgagt tgtccaacag
     4141 gcgttgcccg ggtcttccca gccacgcgtc cgccggtacc tccgcgggcg cggacgacgc
     4201 ggaacgatcc atgatctgcg acgctagcgt gcgaggatcg agcggcagcc cctggtgcag
     4261 cttcgacgcc agcgacgcac cgaacttcga ggagaagcgg cgtgacgtcg caaaccaggc
     4321 gatgctcgcc gacatcgaaa gcaccaccgc gcaggttcgg tcacttctct cgagaacccg
     4381 gatcgcagca tgctcgatgg gcacccacac ggtgatcgat agctgcgcac atagcaagag
     4441 gtctgggtcc cgcgctcgac agagggcgag cgcctcgctc cgcttcgcca acgcgcaggt
     4501 cgccgcgctc accgcgcaga tggaggcgcg ccggaaggcg gcagcgcgct gagaccatcc
     4561 agacccggac gcggcgtcgc cacgaccgcc gccatgctcc ccggggtact gcaccggagt
     4621 cgacgattcc gcggaatcgc cggggagccg accacgattc cgcggaatcg ctgccgtgtc
     4681 cgcccgtgtt ctccgccccc ttccaacatc aacaatggcg ctgggaggct gcgcgcgagg
     4741 cgtcgccatg accgccgcca tgctccccgg gtgccgcacc ggggtcgacg attccgcgaa
     4801 attgccgggg agccgaccac gattccgcgg aatcgctgcc atgtccgcct gcgttctccg
     4861 gcccccccca acatccagca attgcgctgg gagccagcac gcggggcgtc gccacgaccg
     4921 ccgccatgct ccccggggta ctgcaccgga gtcgacgatt ccgcggaacg ctggcgtgtc
     4981 cgccgcgttc tccggatccc cctctccccc caaaatcaat atcggtgagc tgggagcctg
     5041 cgcgcggggc gacggactgt gagcaggcgg tcgaggtcca ccgggtccac tgcctccggc
     5101 tggtcacagg ccacccgcat ggtctcccga ggtccccggc tgcgccaagc tcaggcccgc
     5161 ggcgtaggtc gtgctcgccc gatcgcatcg ccgcgactcg ctcctgcggc acatgccccg
     5221 gtcgcggcat ccggcaggct caaagcgccg gaacgcttag cggctgcggc accgggcggc
     5281 ccctgttgtc gactggcgct gcccgccgtc gacctcgtac agtgtgccgg cgtgctgcag
     5341 cggatcgtcg attccggacg ggtgcccgag ccgatgctgc gcgctgggat ccgggtggtc
     5401 tgcgcgctgc ggctctacca ggagcgccgg cgcccaccgg gctaccggcc cgcgctggtc
     5461 gcggagctcc ggcgcagcga gatcgcgatc gcgcccgacg ccgccaacgc ccagcactac
     5521 gaggtgccgg cggcgttctt cgcccgcgtg ctcggaccgc acctcaagta ctcgtgcgcc
     5581 tactggccgg c
//
LOCUS       DASVOR010000550         1805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_384870,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000550 DASVOR010000000
VERSION     DASVOR010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1805
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384870"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..764
                     /locus_tag="VF516_10925"
     CDS             <1..764
                     /locus_tag="VF516_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108233.1"
                     /translation="QLSLHAADAVVGPLPMTDGTGSAAGMNHAIAAQLSLRAPLGAGL
                     QGSFAAYARRTHHAIDFGMVNQRFTGDDPCHATAGALVYRDIDSRAVGVEAMIRRELG
                     HAVTGWLSYSLGKVDRDLGFVQLPGDFDQRHTLSATAQWQRGRWRFGASGQLHTGRPV
                     PYPLFATCSDGFLTSSTSADALRRLPTTGRIDLRAERALSVGGRPGRLFFELQNATFL
                     PEPLGYRVDGFGAGQRVVEDTLLLPLPLVGLEVVL"
     gene            776..1315
                     /locus_tag="VF516_10930"
     CDS             776..1315
                     /locus_tag="VF516_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108234.1"
                     /translation="MRLGAVMLVAGCATTPDPYAVGAIAPLQPVGTPAGAEAGIDGPI
                     EVLGSPAYGRSFQQTFAAYKVHVPEGAAPWVRVYRTPSCTPVPESPALFDDLGAIRRV
                     GGETHFFAPGVQVAGRIVDIDTRTVTAPGSLDPTYTYGYLIGMIAVVQAPGDPAAGGG
                     PDDPPAGGPWLACGAFEIR"
     gene            1428..>1805
                     /locus_tag="VF516_10935"
     CDS             1428..>1805
                     /locus_tag="VF516_10935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8108235.1"
                     /translation="MTTAVKLAVVAPSPVSDGELVERMAAGDRDALGELFDRHHRALY
                     SFLARASDASEAEIDDLVQTAFLEAFRAAPRFRGAASPRTWLFAIASNLARMHRRATR
                     RRARALRRLADQPERTASPLDAAL"
BASE COUNT          214 a          689 c          656 g          246 t
ORIGIN      
        1 cgcagctgtc gctgcacgcg gccgatgccg tggtcggccc gctgccgatg accgatggca
       61 ccggcagcgc cgccgggatg aaccacgcca tcgcggccca gctgtcgctg cgcgcgccgc
      121 tgggcgccgg gctgcagggc agcttcgcgg cgtatgcccg gcgcacgcat cacgcgatcg
      181 acttcggcat ggtcaaccag cggttcaccg gcgatgaccc gtgccatgcg accgccggcg
      241 cgctggtgta ccgcgacatc gacagccgcg cggtcggcgt cgaggcgatg atccgccgcg
      301 agctcggcca cgcggtgacc ggctggctgt cgtactcgct cggcaaggtc gatcgcgacc
      361 tcggcttcgt ccagctgccc ggcgacttcg atcagcgcca cacgctgagc gcgaccgccc
      421 agtggcagcg cggccgctgg cggttcggcg ccagtggtca gctccacacc ggccgtcccg
      481 tgccgtatcc gctgttcgcg acctgcagcg acggcttcct gacgtcgagc accagcgccg
      541 acgcgctgcg ccgcctgccc accaccggcc gcatcgatct ccgcgccgag cgcgcgctgt
      601 cggtcggcgg ccggccgggg cggttgttct tcgagctgca gaacgccacg tttctgcccg
      661 agccgctcgg ctaccgcgtc gacgggttcg gggcgggcca gcgcgtcgtc gaggacacgc
      721 tgctcctgcc gctgccgctg gtcggcctcg aggtcgtgct gtgaggcccg gcgcggtgag
      781 gctcggcgcg gtgatgctcg tcgccgggtg cgcgaccacg ccggatccgt acgcggtggg
      841 cgcgatcgcg ccgctccagc cggtgggcac gcccgcgggc gccgaggcgg gcatcgacgg
      901 cccgatcgag gtgctcggga gtccagcgta cggccggagc ttccagcaga cgttcgccgc
      961 gtacaaggtg cacgtcccgg agggtgccgc gccgtgggtc cgggtgtacc gaacgccgtc
     1021 gtgcaccccg gtcccggagt cgccggccct gttcgacgac ctcggcgcga tccgtcgggt
     1081 cggcggcgag acccacttct tcgcgccggg cgtccaggtc gccggccgca tcgtcgacat
     1141 cgacacccgg accgtgacgg cgcccgggtc gctggatccc acctacacct acggctacct
     1201 gatcggcatg atcgcggtcg tccaggcgcc cggcgacccc gccgccggcg gcggcccgga
     1261 cgaccctccc gccggcgggc cgtggctcgc ctgcggggcg ttcgagatcc gatgatgcca
     1321 gctcgcgccg cgccccgatc gcgggtagat ccgaagcgcg gccgtgcagc ggcaaccaag
     1381 cgggagtggc cctcgatgat cgcgatcgcc tccagcgagg tcggcgggtg acgaccgccg
     1441 tcaagctcgc ggtggtggcg cccagcccgg tcagcgacgg cgagctggtc gagcggatgg
     1501 ccgcgggcga tcgcgacgcg ctcggcgagc tgttcgaccg ccatcaccgc gcgctgtaca
     1561 gcttcctggc ccgcgcgagc gatgcctcgg aggccgagat cgacgatctg gtccagaccg
     1621 cgttcctcga ggcgttccgc gccgcaccgc ggttccgggg cgccgcatcg ccgcgcacct
     1681 ggctgttcgc catcgcgagc aacctcgcga ggatgcatcg ccgcgccacc cggcggcggg
     1741 cacgcgcgct gcgccggctc gccgatcagc ccgagcgcac ggcgagcccg ctcgacgccg
     1801 cgctc
//
LOCUS       DASVOR010000551         3919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_151205,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000551 DASVOR010000000
VERSION     DASVOR010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151205"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="VF516_10940"
     CDS             complement(<1..194)
                     /locus_tag="VF516_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX8108236.1"
                     /translation="MLHKLLIANRGEVAVRIAQAAAELGIATVAVYSDLDRDALHVRL
                     ADEAYALGGTTAADSYLNTE"
     gene            351..1424
                     /locus_tag="VF516_10945"
     CDS             351..1424
                     /locus_tag="VF516_10945"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEX8108237.1"
                     /translation="MLAQLEGALTAPWTITIAAVVIAFLVGRVIVRLARPLLARMAGR
                     TSVDWDDRLVELMAWPLSLVLALQALRIASPWLPLDARATTTLNDAVAIVTTLAVLWT
                     AFRGIDLGRNVLERRSWAIDRPSTRSLLHIGARLAKVSVLLIAGIVVLAQLGVSVASL
                     IAGLGIGGLAVALAAQKTLENLFGTLSIGVDQPMREGDVVKLYDFTGTVEQIGLRSTR
                     IRTVDRTIITIPNGELANQRIESFTVRDRLRFATNLGLVYGTTASQIRAIRGEIDAIL
                     RRHPKVCHDTVAVWFKELGATALVLEVNALFQTIDWNEFVTIRQDLLLAFLVAIEQHG
                     SAIALPTQTVHFMGPGHPAPPAG"
     gene            1478..3055
                     /locus_tag="VF516_10950"
     CDS             1478..3055
                     /locus_tag="VF516_10950"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HEX8108238.1"
                     /translation="METPLSPLDLMRRTRRLYPHHEAVVDGELRLSYEQFFARCDRWS
                     SALQRELGVRQGDRVAYIAPNTHAQLTSFYAVPQLGAVLVPINYRLTADDFAYIINHS
                     GSTVVCVHSDYLETVDKIRDQLTGVVHFVALEGARDGWLDYEQLIAAASGELTRPQIG
                     ERDLLTINYTSGTTARPKGVMITHRNAYMNVLGTILHVPMAATAERYLWTLPMFHANG
                     WTFVWTITAVGGRHVCLRRVEPPRIFAELARERITMLCAAPTVLIGIANAPEELRKGA
                     PAGVRVLTAGAPPAAATINRVEQELGWVITHVYGLTETAPFITVCEPRPEHAGLPAAE
                     LAVLKARQGVELISSGELLVVGEDGREVPHDGQSIGEIVVRGNVVMEGYYNDPEATAT
                     ALRGGYFHSGDAAVVHPDGYVEVRDRIKDVIISGGENISSVEVEGVLLRHPSVQEVAV
                     VGMPSEKWGESPHAFVILRAGASATEDELRAFTRQHLAHFKCPQVFHFVTELPKTATG
                     KIQKFVLRGRRAGIAAQ"
     gene            complement(3223..>3919)
                     /locus_tag="VF516_10955"
     CDS             complement(3223..>3919)
                     /locus_tag="VF516_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108239.1"
                     /translation="SPDGRRLAVLADDEPAAAPGAMRSAPRPGRLLLVIDRSGAVISR
                     LPVDDVDPEAAPVWLDDHRIAVRTGDRLTYRGIDLVTGDQGEILDRRRGSTLWLTRSP
                     RDGTLAMFRMGAPAAAGAGPIEHLWIQPPGGDPRPLHVDDVKHFLAPSWTASGELVVR
                     ALETGEVSRVALDTGELTPIAQLAPTPLTLMSDDHLVMPPGGDLLAIHRELGANVAKV
                     RLDDTAPAAAGSP"
BASE COUNT          567 a         1350 c         1438 g          564 t
ORIGIN      
        1 ttctcggtgt tgaggtagct gtcggccgcc gtcgtgcccc cgagggcgta ggcctcgtcg
       61 gcgaggcgca cgtggagggc gtcgcggtcg aggtcggagt acaccgcgac ggtcgcgatg
      121 ccgagctccg ccgccgcctg cgcgatccgg accgccacct cgccgcggtt ggcgatgaga
      181 agcttgtgca gcatgtcggt gggggcggat ggtgagacag atggcccggg cgcgcaacac
      241 cggcgagcac ggccgcggcc gctgccgtag actcggctcc ttcttgatga ggaggaagcc
      301 gtgatccagc tccggagacg ctcgccgtga ccggcggcgc gaacgatggg atgctcgcgc
      361 agctcgaggg ggcgctgacc gcgccgtgga cgatcacgat cgccgcggtc gtgatcgcgt
      421 tcctggtcgg ccgggtcatc gtgcggctcg cgcggccgct gctcgcccgg atggcggggc
      481 ggaccagcgt cgactgggac gaccggctgg tcgagctgat ggcgtggccg ctcagcctgg
      541 tgctcgcgct ccaggcgctg cggatcgcgt cgccgtggct gccgctcgac gcgcgcgcga
      601 ccacgacgct caacgacgcg gtcgcgatcg tgaccacgct ggccgtactg tggaccgcgt
      661 tccgcggcat cgacctcggg cgcaacgtgc tcgagcggcg gtcgtgggcg atcgaccggc
      721 cgtcgacgcg ctcgctgctg catatcggcg cgcggctggc caaggtctcg gtgctgctga
      781 tcgccgggat cgtcgtgctc gcccagctcg gggtgtcggt cgccagcctg atcgccggcc
      841 tggggatcgg cgggctcgcc gtcgcgctgg cggcgcagaa gacgctggag aacctgttcg
      901 gcacgctgtc gatcggggtc gatcagccga tgcgcgaggg cgacgtggtc aagctctacg
      961 acttcaccgg cacggtcgag cagatcggcc tgcgctcgac ccggatccgc acggtcgacc
     1021 gcacgatcat cacgatcccc aacggcgagc tcgccaacca gcggatcgag tcgttcaccg
     1081 tgcgcgatcg gctgcggttc gcgaccaacc tcggcctggt ctacggcacg acggcgtccc
     1141 agatccgcgc gatccgcggc gagatcgacg cgatcctgcg ccgccacccc aaggtctgcc
     1201 acgacaccgt ggcggtgtgg ttcaaggagc tcggcgctac ggcgctcgtg ctcgaggtca
     1261 acgcgctgtt ccagaccatc gactggaacg agttcgtgac catccgccag gacctcctgc
     1321 tcgcgttcct ggtcgcgatc gagcagcacg gcagcgcgat cgcgttgccg acccagaccg
     1381 tccacttcat gggacccggt cacccggcgc cgcccgccgg gtgaccggtc cgcaaatcgc
     1441 gacagcggcc gatgccggac gtgcgatgct gggccgcatg gagaccccgc tgagcccgct
     1501 cgacctgatg cgccggaccc ggcgcctgta cccgcaccac gaagccgtcg tcgacggcga
     1561 gctccggctg tcgtacgagc agttcttcgc gcgctgcgat cgctggtcgt cggcgctgca
     1621 gcgcgagctc ggggtgcgcc agggcgatcg cgtcgcatac atcgcgccca acacccacgc
     1681 ccagctgacg tcgttctacg ccgtgccgca gctcggcgcc gtgctggtgc cgatcaacta
     1741 ccggctgacg gcagacgact tcgcgtacat catcaaccac agcggctcca cggtggtgtg
     1801 cgtgcactcc gactacctgg agaccgtcga caagatccgc gatcagctga ccggcgtcgt
     1861 ccacttcgtc gcgctcgagg gggcgcgcga cggctggctc gactacgagc agctgatcgc
     1921 ggcggcgagc ggcgagctca cccggccgca gatcggcgag cgcgatctgc tgacgatcaa
     1981 ctacacgagc ggcacgacgg cacggcccaa gggcgtgatg atcacgcacc gcaacgcgta
     2041 catgaacgtg ctcggcacga tcctccacgt gccgatggcg gcgaccgccg agcgctacct
     2101 gtggaccctg ccgatgttcc acgccaacgg ctggaccttc gtgtggacga tcacggcggt
     2161 cggcggccgc cacgtctgcc tgcgccgggt cgagccgccg cggatcttcg ccgagctcgc
     2221 gcgcgagcgg atcacgatgc tgtgcgcggc gccgaccgtg ctgatcggca tcgccaacgc
     2281 gcccgaggag ctgcgcaagg gcgcgccggc cggggtccgc gtgctgaccg ccggggcgcc
     2341 gccggcggcc gccacgatca accgggtcga gcaggagctc ggctgggtga tcacccacgt
     2401 ctacgggttg accgagaccg cgccgttcat cacggtgtgc gagccgcggc ccgagcacgc
     2461 cgggctgccg gcggccgagc tcgcggtgct caaggcgcgc cagggcgtcg agctgatctc
     2521 gtcgggcgag ctcctggtgg tcggcgagga cggccgcgag gtgccgcacg acggccagtc
     2581 gatcggcgag atcgtcgtgc gaggcaacgt cgtgatggag ggctactaca acgatcccga
     2641 ggccaccgcg accgcgctgc gcggcggcta cttccactcc ggcgacgccg cggtggtcca
     2701 ccccgacggc tatgtcgagg tccgcgaccg gatcaaggac gtgatcatca gcggcggcga
     2761 gaacatctcg tcggtcgagg tcgagggcgt gctgcttcgc catccctcgg tgcaggaggt
     2821 cgcggtggtc ggcatgccga gcgagaagtg gggcgagagc ccgcacgcgt tcgtgatcct
     2881 gcgcgccggc gcgagcgcga ccgaggacga gctgcgcgcg ttcacccgcc agcacctcgc
     2941 gcacttcaag tgcccgcagg tgttccactt cgtcaccgag ctgcccaaga ccgcgaccgg
     3001 caagatccag aagttcgtgc tgcgcggccg gcgcgccggg atcgccgcgc agtagggata
     3061 tgtcacggga gagctttctt ctcttctgca gcaggtgaca atctcagaag atgaggaatg
     3121 ccaaagtcaa gtaaggaaga tcttcgataa gattggtaag aaatgatcga aggtgcttcg
     3181 agaggagcgt gacgggcctc ctggtcgagg cgtcggcgct gcctacgggg acccggcggc
     3241 cgccggcgcg gtgtcgtcga gccggacctt ggcgacgttg gcgccgagct cgcggtggat
     3301 cgccagcagg tcgccgccgg ggggcatcac gaggtggtcg tcggacatca gggtgagcgg
     3361 ggtcggtgcg agctgcgcga tcggggtgag ctcgccggtg tcgagcgcga cgcgcgacac
     3421 ctcgccggtc tccagcgcgc gcaccacgag ctcgcccgac gcggtccagc tcggggcgag
     3481 gaagtgcttg acgtcgtcga cgtggagcgg ccgcgggtcg ccgcccggtg gctggatcca
     3541 caggtgctcg atcgggcccg caccggcggc ggccggcgcg cccatgcgga acatcgccag
     3601 cgtgccgtcg cgcggcgagc gggtcagcca cagggtcgag ccgcgccggc gatcgaggat
     3661 ctcgccctgg tccccggtga cgagatcgat cccgcggtag gtgagccggt ccccggtccg
     3721 gaccgcgatc cggtggtcgt ccagccacac cggcgcggcc tcgggatcga cgtcgtcgac
     3781 cggcaggcgc gagatcacgg cgccgctgcg gtcgatgacc agcaggagcc tccccggccg
     3841 gggcgcggag cgcatcgcgc cgggcgcggc ggcgggctcg tcgtcggcca gcaccgcgag
     3901 ccggcggccg tcgggggac
//
LOCUS       DASVOR010000552         1535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_472801,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000552 DASVOR010000000
VERSION     DASVOR010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1535
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472801"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..692
                     /locus_tag="VF516_10960"
     CDS             <1..692
                     /locus_tag="VF516_10960"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX8108240.1"
                     /translation="IDDAAADPQAVAELGGDAGAASVACVPLLYGEIAVGALEAVAGP
                     GALSDEDVETLRILGQIIAIALHRAYTYPRPRYDSLQDALTGLGNRRAFDERLDAELN
                     RNRRFGHSFSLALVRLAGLETAIDRHGQAAGDEMLRRIATILKEHTRMIDGCFRIRAD
                     ELAIVMPGTSLHGAQIVTERCRAHSAEAKVCDGGVRLGFGVVAAVEETASQILDRAAA
                     ALAQDTAPVDA"
     gene            complement(689..961)
                     /locus_tag="VF516_10965"
     CDS             complement(689..961)
                     /locus_tag="VF516_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108241.1"
                     /translation="MTATRFTYRRLVELVDGDDELIVQLVDEGVIEPCGGDRVTVDVD
                     SVLAAQTLRELEVDWSGIPIILRLRAELANARRRIAELEAAADRER"
     gene            complement(958..>1535)
                     /locus_tag="VF516_10970"
     CDS             complement(958..>1535)
                     /locus_tag="VF516_10970"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8108242.1"
                     /translation="EFDFAELFNRRRGPARGGDIHATFQMDLRQAIEGTEVSVDVPEQ
                     GTVRVRIPPGADTGSVIRLPGKGAPGPRGGPPGDLVIETEVRPHPLIRRDGLDLYLTL
                     PVTLDEAYNGASVDVPTFEGTVVLKIPPRSQNHARLRLRGKGIERKSTRGDFFVELDV
                     RMPDRADDALADALRAASAHYGKPVREGLTL"
BASE COUNT          197 a          538 c          596 g          204 t
ORIGIN      
        1 ggatcgacga tgccgccgcg gatccgcagg cggtcgccga gctcggcggg gacgccggcg
       61 cggcctcggt ggcctgcgtg ccgctcctgt acggcgagat cgcggtcggc gcgctcgagg
      121 cggtcgccgg gcccggcgcg ctgtccgacg aggacgtcga gaccctgcgg atcctcggcc
      181 agatcatcgc gatcgcgctg caccgcgcgt acacctatcc gcggccgcgc tacgacagcc
      241 tgcaggacgc gctgaccggg ctgggcaacc ggcgcgcgtt cgacgagcgg ctcgacgccg
      301 agctcaaccg caaccggcgg ttcgggcact cgttcagcct cgcgctggtc cggctcgccg
      361 gcctggagac cgcgatcgac cgccacggcc aggccgcggg cgacgagatg ctgcgccgga
      421 tcgcgacgat cctgaaggag cacacccgga tgatcgacgg ctgcttccgg atccgcgccg
      481 acgagctggc gatcgtgatg ccggggacgt cgctgcacgg cgcccagatc gtgaccgagc
      541 gctgccgcgc tcacagcgcc gaggccaagg tctgcgacgg cggggtgcgg ctcgggttcg
      601 gcgtggtcgc cgcggtcgag gaaactgcat cgcagatcct cgaccgcgca gccgccgcgc
      661 tggcgcagga caccgcgccg gtggatgcct agcgctcgcg gtccgcggcg gcctcgagct
      721 cggcgatccg gcggcgggcg ttcgcgagct cggcgcgcag ccgcaggatg atcgggatgc
      781 cggaccagtc gacctcgagc tcgcgcaggg tctgggcggc gagcacgctg tcgacgtcga
      841 cggtgacccg gtcgccgccg cacggctcga tcacgccctc gtcgaccagc tggacgatca
      901 gctcgtcgtc gccgtcgacc agctcgacca gccgccggta ggtgaagcgc gtggcggtca
      961 tagcgtcaac ccctcgcgaa cgggctttcc gtagtgcgcg ctggcggcgc gcagcgcgtc
     1021 ggcgagcgcg tcgtcggcgc ggtccggcat gcggacgtcg agctcgacga agaagtcgcc
     1081 gcgggtcgac ttgcgctcga tgcccttgcc gcgcagccgg agccgggcgt ggttctgcga
     1141 gcgcggcggg atcttgagca cgaccgtgcc ctcgaaggtc ggcacgtcga cgctcgcgcc
     1201 gttgtaggcc tcgtcgagtg tgaccggcag cgtgaggtag aggtcgaggc cgtcgcggcg
     1261 gatcagcggg tgcggccgga cctcggtctc gatcacgagg tcgccgggcg ggccgccgcg
     1321 cgggccgggg gcgcccttgc cgggcagccg gatcaccgag ccggtgtcgg cgccgggcgg
     1381 gatccggacg cggaccgtgc cctgctcggg gacatcgacc gagacctcgg tgccctcgat
     1441 ggcctggcgg aggtccatct ggaatgtcgc gtggatgtcg ccgccgcggg ccgggccgcg
     1501 gcggcggttg aacagctcgg cgaagtcgaa ctcga
//
LOCUS       DASVOR010000553         3146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_122012,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000553 DASVOR010000000
VERSION     DASVOR010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122012"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VF516_10975"
     CDS             complement(<1..1517)
                     /locus_tag="VF516_10975"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8108243.1"
                     /translation="MDEQQAKRGGASRFLWILVLLAVVVLWWYFRPESHHAVSRVDEQ
                     AEANTAPIEPDEILVDLRDDATPAMISAIERDAGIQLVLVDDTAAATKLYRAHVDPDR
                     RDAVLRQLKARPEVEIAEPDFQMQLSPYDFLPPAVVPDAKPGEKGFPNDPMYKHQWHL
                     DQIGMPKAWPLADGSGVIVAVLDTGVAYEDYKNFHVLPDLKGISFVDPYNFVANNNHA
                     DDDHGHGSHVTGTIAQVTHNSIGVAGIARNVKIMPLKVLSGSGSGSVAGIADAIRFAA
                     DHGAKVINMSLGGAFPSAVLKKAVAYAHGKGVTVVCAAGNESRGRVGYPAAYPGAIAV
                     SATQFDEAITFYSNYGKDIDIAAPGGNTRVDQNGDGMPDGVLQNTIALGDPTKDDYFG
                     YMGTSMASPHVAGVAALIVGEGVTDPDQVEQVLEASARKPAQGYKEDRYGAGIVDAPA
                     AVLKARSSTGGLQLALGALMAGAIAVSLRRRGSLGIKIGPSYVLGVLFGASGLFFL"
     gene            complement(1613..2917)
                     /locus_tag="VF516_10980"
     CDS             complement(1613..2917)
                     /locus_tag="VF516_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX8108244.1"
                     /translation="MVSLGDVKRSAATLFAQGQYLAALRLYDAAVAAAPLDVDARIRV
                     ADCALALGDPGAARVYRAAGWYCLRSGHPLSALVCARVLEAHGADASDLTAALVVTYG
                     SESDLLGKGAARIPLPPDTLAISVPDVRQPAPPDAVRTALDRAEHAAASAELPSALHP
                     IPLLSALSEAAFRRVLGTLVLRRLPAGALVIREGEPGEAFFFVASGRLRVYATDGLGR
                     QTELAELGESAVFGEMALLSAQPRSASVASLTEVDLLEVGRQSLAQLADELGPVAEAL
                     HGFTRERLLGNLMATSPLFRPFPRLQQRDLLRRFTSHDVAPGTVVIREGEQGRGLFVV
                     LSGELDVSRRGPDGQPVPLGGLRTGDVFGEMAILRNAATTATVVCVRQATVLFLAREY
                     VARIVAAVPEIKSYLEALAEDREIDNQLVMGEDAPADERILI"
BASE COUNT          457 a         1125 c         1121 g          443 t
ORIGIN      
        1 aggaagaaca ggcccgaggc gccgaacagc acgccgagca cgtacgacgg gccgatcttg
       61 atgccgaggc tgccgcgccg gcggagcgac acggcgatcg caccggccat cagcgcgccg
      121 agcgcgagct ggaggccgcc ggtcgagctg cgcgccttga gcaccgcggc cggggcatcg
      181 acgatgccgg cgccgtagcg gtcctccttg tagccctgcg ccggcttgcg cgcgctggcc
      241 tcgaggacct gctcgacctg atcggggtcg gtcacgccct cgccgacgat cagcgcggcg
      301 acgccggcga cgtggggcga cgccatcgac gtgcccatgt agccgaagta gtcgtccttg
      361 gtcggatcgc cgagcgcgat cgtgttctgc agcacgccgt cgggcatgcc gtcgccgttc
      421 tggtcgacgc gcgtgttccc gcccggcgcc gcgatgtcga tgtccttgcc gtagttcgag
      481 tagaacgtga tcgcctcgtc gaactgggtc gcgctgaccg cgatcgcacc cgggtaggcc
      541 gccgggtagc cgacccggcc gcggctctcg ttgccggcgg cgcacaccac ggtgacgccc
      601 ttgccgtgcg cgtaggcgac cgccttcttg agcaccgccg acgggaacgc gccgcccagg
      661 ctcatgttga tcaccttggc gccgtgatcg gcggcgaacc ggatcgcgtc ggcgatgccg
      721 gcgaccgagc ccgaccccga ccccgacagc accttgagcg gcatgatctt gacgttgcgg
      781 gcgatgccgg cgacgccgat gctgttgtgc gtgacctgcg cgatcgtgcc ggtgacgtgc
      841 gagccgtggc cgtggtcgtc gtcggcgtgg ttgttgttgg cgacgaagtt gtacgggtcg
      901 acgaaggaga tgcccttgag gtcgggcagg acgtggaagt tcttgtagtc ctcgtaggcg
      961 acgccggtgt cgagcaccgc gacgatcaca ccgctgccgt cggcgagcgg ccacgccttg
     1021 ggcatgccga tctgatcgag gtgccactgg tgcttgtaca tcgggtcgtt ggggaacccc
     1081 ttctctcccg gcttcgcgtc cggaacgacc gcgggcggca ggaagtcgta cggcgacagc
     1141 tgcatctgga agtccggctc ggcgatctcg acctcgggcc gggccttgag ctggcgcagg
     1201 accgcgtcgc ggcgatcggg gtcgacgtgg gcgcggtaca gcttggtcgc cgccgcggtg
     1261 tcgtcgacca ggacgagctg gatgccggcg tcgcgctcga tcgccgagat catcgccggc
     1321 gtcgcgtcat cgcgcagatc gaccaggatc tcgtcgggct cgatcggagc ggtgttggcc
     1381 tcggcctgct cgtcgacccg gctgaccgcg tggtggctct ccgggcggaa gtaccaccac
     1441 agcacgacga ccgcgaggag cacgaggatc cacaggaacc ggctggctcc accgcgcttg
     1501 gcttgttgct cgtccatgca cgaagtatag gcccggcggc tcgcctccgc ccgtacgacc
     1561 gcatcgccgg ccctgagaat ccctggggcc tcacctctcc ggcacctgcc catcagatga
     1621 ggatgcgctc gtcggccggg gcgtcctcgc ccatcaccag ctggttgtcg atctcgcggt
     1681 cctccgccaa tgcttcgaga tagctcttga tttcggggac cgccgcgacg atccgcgcga
     1741 cgtactcgcg ggcgaggaac agcacggtcg cctggcgcac gcagaccacc gtcgcggtcg
     1801 tggcggcgtt gcgcaggatc gccatctcac cgaacacgtc gccggtgcgc aggccgccga
     1861 gcggcaccgg ctgaccgtcg gggccgcgcc gcgacacgtc gagctcgccg gacagcacga
     1921 cgaacaggcc gcggccctgc tcgccctcgc ggatcaccac ggtgccgggc gcgacgtcgt
     1981 ggctggtgaa ccggcgcagc aggtcgcgct gctgcagccg cgggaacggc cggaacagcg
     2041 ggctggtcgc catcaggttg ccgagcaggc gctcgcgggt gaagccgtgc aacgcctcgg
     2101 cgaccggacc gagctcgtcg gcgagctggg ccagcgactg gcggccgacc tcgagcaggt
     2161 cgacctcggt caggctcgcc acgctcgccg agcgcggctg cgccgacaag agggccatct
     2221 cgccgaacac cgcgctctcc ccgagctcgg cgagctcggt ctggcggccc agcccgtcgg
     2281 tcgcgtacac ccgcagccgg ccgctcgcca cgaagaagaa cgcctcgccg ggctcgccct
     2341 cgcggatcac cagcgccccg gccggaagcc ggcgcagcac cagcgtgccg agcacgcggc
     2401 gaaacgccgc ctccgacagc gccgacagca gcgggatcgg atgcagcgcc gacggcagct
     2461 cggcgctcgc cgcggcgtgc tcggcgcgat cgagcgctgt tcgaaccgcg tccggcggcg
     2521 ccggctggcg gacatcgggg accgagatcg ccagggtatc cggcggcagc gggatccgcg
     2581 ccgccccctt gcccagcaga tcggactcgc tgccgtaggt cacgaccagc gccgcggtca
     2641 gatcgctggc gtcggcgccg tgcgcctcca gcacgcgcgc gcagaccagc gcgctcaagg
     2701 gatgaccgga cctgaggcaa taccagccag ccgcgcggta cacccgcgcc gcgcccggat
     2761 cgcccagcgc gagcgcgcag tccgcgaccc ggatccgcgc gtcgacgtcg agcggtgcgg
     2821 ctgcgacggc ggcgtcatac agccgcagcg cggcgaggta ctgcccctgc gcgaacagcg
     2881 tcgctgcgct gcgcttgaca tcgccgagcg aaaccatgac ggtaccgtac acgaggccgg
     2941 gtttgcagcg cgcgacccgg cggacctgga ggtatactcg ggtgtcgcag ggggactcgt
     3001 gaagaaatgc gctagctgca ccaaggatct tccggacgcc gcgctgcact gcgtgttctg
     3061 cggcgccaag cagccccccg cacccgccgt gcagcccggc ctcggcaaga ccgcgttcgg
     3121 ctactcggcg aacgaggtac tcaagg
//
LOCUS       DASVOR010000554         3629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_502002,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000554 DASVOR010000000
VERSION     DASVOR010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502002"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..505
                     /locus_tag="VF516_10985"
     CDS             <1..505
                     /locus_tag="VF516_10985"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8108245.1"
                     /translation="LHQAGTPRRTIAFVAFGGEELGELGSRHFAAHPPAALPLDRVVY
                     DVNLDMLGSYASTGAVHAMGTFRSLPATAIVRALAAEQPALHVGLGGRGVGSDHEAFC
                     RAGVPYVFFWTPDRRCYHTRCDTTDRLDTAHLAAIAQLAGALVARLADTPVDLAASRT
                     RLGCGSR"
     gene            complement(520..1359)
                     /locus_tag="VF516_10990"
     CDS             complement(520..1359)
                     /locus_tag="VF516_10990"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX8108246.1"
                     /translation="MSYADLLARVPMFENLADDDRQALAQSLVERKFAAGDIIMNLGD
                     RGTSMFIVAEGHVNIYLPGEASRRISLKDITTGEHLGEVALFDDRPRSASAVATTDAT
                     LLELDRDTLSRYLAQRPQGAMTLLRALAARLRATNEMLSQRAAKNVVAELESKLTWSQ
                     KLADRVASLNGSWAFILILVGLTLGWIVVNDPAVAGVDPYPYQFFNLLLAILVALQGP
                     LIVMSQNRSSLKDRAQAETDFQVNLKNEVNIETLLRELGEMRAEMNQRLLALETAPRR
                     ASA"
     gene            complement(1455..2009)
                     /locus_tag="VF516_10995"
     CDS             complement(1455..2009)
                     /locus_tag="VF516_10995"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8108247.1"
                     /translation="MPDVIVIGDGPGGLSAALFLAKNKLDVVVFGQDKTAMNYALLRN
                     YLGIPEILGTEFQRIARKQVAELGARLRGDAVEAIVKNGDGWQVTLEGGERQTAKFLV
                     LSEGKGPRLARQLGLTFDEHNGIATDRNARSSLDGVYIVGRSARPGRSQAIISAGDGA
                     AAAIDILSRVHGENFVDWDEPPKA"
     gene            complement(2530..2928)
                     /locus_tag="VF516_11000"
     CDS             complement(2530..2928)
                     /locus_tag="VF516_11000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108248.1"
                     /translation="MPYAFLSDEWFTKVEELIAAAGDLQIPPAMKVVEVNVTVTGSAM
                     AVSGEVRLAMKEGLFTRGHRSDFATTLTLSADLARKIFLDGDTAAGVQAFLDGSILVD
                     GDLAKLVAMQTVEPSEPQKRLTRQIAAITQ"
     gene            2982..>3629
                     /locus_tag="VF516_11005"
     CDS             2982..>3629
                     /locus_tag="VF516_11005"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108249.1"
                     /translation="MEYAFGDITIACSNVDRVMFPGDGITKGEVIAYYRDVAALIVPE
                     LRGRPLTIVRYTKGIDRGGFYQKHYQKHFPAWLDRVAAGTRTVVEYPVVDSEAGVVYL
                     ANQGALELHIWTSRKDDLHRPDLLVFDLDPPDGRFDLARRAAGLVRAVLEQLGLPAFV
                     KLTGGKGLHVIAPVERSETGGGDPDGSAGGAGGGAPRGIDQLRGAIETIQRDPSSR"
BASE COUNT          520 a         1261 c         1288 g          560 t
ORIGIN      
        1 gctgcaccag gccggcacgc cgcggcgcac gatcgcgttc gtcgcgttcg gcggcgagga
       61 gctcggcgag ctcggctcgc gccacttcgc cgcgcacccg ccggcggcgc tgccgctcga
      121 ccgcgtggtc tacgacgtca acctcgacat gctcggcagc tatgcgtcga ccggcgcggt
      181 ccacgccatg ggcacgttcc gcagcctgcc ggcgaccgcg atcgtccggg cgctggcggc
      241 cgagcagccg gcgctgcacg tcgggctcgg cggccgcggc gtcggctcgg accacgaggc
      301 gttctgtcgc gccggcgtgc cctacgtgtt cttctggacc ccggaccggc ggtgctacca
      361 cacgcgctgc gacacgaccg accggctgga caccgcgcac ctcgccgcga tcgcgcagct
      421 cgccggcgcg ctggtcgcac ggctggccga cacccccgtc gatctggccg cgtcgcgcac
      481 ccggctgggc tgcggcagcc ggtagcgctc cctgccgggt caggcgctgg cgcggcgcgg
      541 cgcggtctcc agcgcgagca gccgctggtt catctcggcg cgcatctcgc cgagctcgcg
      601 cagcagggtc tcgatgttga cctcgttctt gaggttgacc tggaagtcgg tctcggcctg
      661 cgcgcggtcc ttgagcgacg agcggttctg gctcatcacg atcagcgggc cctgcagcgc
      721 gaccaggatc gcgagcagca ggttgaagaa ctggtacgga tacggatcga cgccggcgac
      781 cgccgggtcg ttgaccacga tccagcccag ggtcagcccg accaggatca ggatgaacgc
      841 ccagctgccg ttgagcgagg cgacccggtc ggcgagcttc tggctccagg tcagcttgct
      901 ctcgagctcg gcgacgacgt tcttggcggc gcgctgcgac agcatctcgt tggtcgcgcg
      961 cagccgggcg gccagggcgc ggagcagcgt catcgcgccc tgcggccgct gggcgaggta
     1021 gcggctcagc gtgtcgcggt cgagctcgag cagcgtcgcg tcggtggtcg cgaccgcgct
     1081 ggccgaccgc gggcggtcgt cgaacagcgc gacctcgccg aggtgctcgc cggtcgtgat
     1141 gtccttgagc gagatccggc gcgacgcctc gcccggcagg tagatgttga cgtgaccctc
     1201 ggcgacgatg aacatcgacg tcccgcggtc gccgaggttc atgatgatgt caccggccgc
     1261 gaacttgcgc tcgaccaggc tctgggccag cgcctggcga tcgtcgtcgg ccaggttctc
     1321 gaacatcggc acccgggcca gcaggtcagc gtagctcatg gccgatcgat atcccggatg
     1381 gccgccgcgc tccagttacg atgcccagta ggatgtggtg atgccggcag cgccgggcaa
     1441 cgcttgccgc ccggtcacgc cttgggcggc tcgtcccagt ccacgaagtt ctcgccgtgg
     1501 acccgcgaca ggatgtcgat cgccgccgcc gcgccgtcgc cggcgctgat gatcgcctgg
     1561 ctgcgcccgg ggcgcgccga ccggccgacg atgtacacgc cgtcgaggct cgaccgcgcg
     1621 ttgcgatcgg tcgcgatccc gttgtgctcg tcgaacgtca gcccgagctg cctggcgagc
     1681 cgcgggccct tgccctcgga cagcaccagg aacttcgcgg tctggcgctc gccgccctcg
     1741 agcgtcacct gccagccgtc gccgttcttc acgatggcct cgaccgcgtc gccgcgcagc
     1801 cgcgcgccga gctcggcgac ctgcttgcgg gcgatccgct ggaactcggt cccgaggatc
     1861 tcggggatgc cgaggtagtt gcgcagcagc gcgtagttca tcgcggtctt gtcctggccg
     1921 aacaccacga cgtcgagctt gttcttggcc agaaacagcg cggcgctcag tcccccgggg
     1981 ccatcaccga tcacgatcac atcgggcatc gcctactcct tgttgcgagc cgccagcatg
     2041 gcacggtcgg cgcgcgaggt cagcgggcgg agcccgagca gcggcggtgg gcgcgcgatg
     2101 cccacgcagc gctccgagcg caccttatgg cttgcagtag cccttcatgt tgcagaaggg
     2161 gcccatcgat ccattcgggc acgacgggtc cgtcgccttg agcatcgtgc acagctgcga
     2221 gcagacgggg tggccggcca tgtcgatggt gttggtgcag gccttcccca tgcactcgct
     2281 ggaggtgtcg gagggcgtgg tacacgcagc gccgaatgcc cccggtgcgg gaccggacgc
     2341 gtccggggcg ggcgccggcg agttgccgga gcaggcgcac agggccaggg acaaggcgag
     2401 cacgagacgc tgcatggtcg gcaacgttac aatggctcgc ccgtcgaccg gccgacgcag
     2461 ttgtgacctg gacgttacac cgcgtgaccg ggcgatcacc ggatctatcc gcgatcggcc
     2521 gacgccgcgt cactgcgtga tcgccgcgat ctggcgagtc agccgcttct gcggctcgct
     2581 gggctcgacg gtctgcatcg cgaccagctt ggcgaggtcg ccgtcgacaa ggatcgagcc
     2641 gtcgaggaac gcctggacgc cggccgcggt gtcgccatcg aggaagatct tgcgggcgag
     2701 gtcggccgac agcgtgagcg tggtcgcaaa gtcggagcgg tgaccgcggg tgaacaggcc
     2761 ttccttcatc gcgagccgga cctcgcccga gacggccatc gcagagccgg tcaccgtgac
     2821 gttgacctcg accaccttca tcgccggcgg gatctgcaga tcgccggccg ctgcgatgag
     2881 ctcttcgacc ttggtgaacc attcatcgga caggaacgcg tagggcatga cgaaccataa
     2941 cgcactgcgt gaaacgaggg gcgttcgggt atgctggcgg cgtggagtac gccttcggcg
     3001 acatcacgat cgcgtgcagc aacgtcgatc gcgtgatgtt tcccggcgat ggcatcacca
     3061 agggtgaggt catcgcctac taccgcgacg tcgccgcgct gatcgtcccc gagctgcgcg
     3121 gacggccgct caccatcgtt cgctacacca agggcatcga ccgaggcggg ttctatcaga
     3181 agcactacca gaagcacttc cccgcatggc tcgaccgggt cgcggccggg accaggacgg
     3241 tggtcgagta tccggtcgtc gacagcgagg ccggcgtggt ctacctcgcg aaccagggcg
     3301 cgctcgagct ccacatctgg accagccgca aggacgatct ccaccggccc gacctcttgg
     3361 tgttcgacct cgatccaccg gacggtcggt tcgatctggc gcggcgcgcc gcgggcctgg
     3421 tccgcgcggt gctcgagcag ctcgggctgc cggcgttcgt caagctgacc ggcggcaagg
     3481 gcctacacgt gatcgcgccg gtcgagcgca gcgagacggg gggtggggac cccgacggct
     3541 ctgccggcgg ggcggggggc ggagccccac gtgggatcga ccagctccgg ggcgcgatcg
     3601 agaccatcca gcgcgatccg tcgtcgcgc
//
LOCUS       DASVOR010000555         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_267945,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000555 DASVOR010000000
VERSION     DASVOR010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267945"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VF516_11010"
     CDS             <1..236
                     /locus_tag="VF516_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108250.1"
                     /translation="FVTADAAVCAAGSTLAELAYLGCPALGFAIVPDQVLPATSQARG
                     GLIAGGREPPLSSGRGPARCRRSARPSRRGCRR"
     gene            complement(166..1284)
                     /locus_tag="VF516_11015"
     CDS             complement(166..1284)
                     /locus_tag="VF516_11015"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX8108251.1"
                     /translation="MREHLAITTAVGAAALLGAAVVAGVARGDDDRSEDRQGAFQVTN
                     LVSNIPNLAKVTDHNTVNPWGAAFIPGEALWVADNGSHALTVYSLNPKDMPQVGKLVV
                     KLSPQDNTWAPTGLVWNPSPNQFFLPNTKFPATFIAVSEGGQIVGWNANANPPDPVGL
                     SLATVIKTNAGGVYKGAAFGTNPEGSFIFVTNFRAAKVEAYDTNFTEVTRFPFIDRDI
                     PSGFAPFGIENICGELFVTFAKQDKAKEDDVAGPGLGFVDVFTTSGKLVRRFATRGTL
                     NAPWGLARAPLSFGKFGGAILVGNFGDGKMNAFGNHGEFLGQLRARNGKTITIDGLWA
                     LKFGGFKGAEPEDLYFTAGIHDEKDGLIGDISPVPGRN"
BASE COUNT          276 a          688 c          625 g          343 t
ORIGIN      
        1 tgttcgtcac cgccgacgcc gcggtctgcg cggcgggcag cacgctcgcc gagctcgcct
       61 acctcggctg tcccgcgctc ggcttcgcca tcgtccccga ccaggtcctg ccggccacca
      121 gccaggcgcg cggcggcctg atcgccggcg gccgcgagcc gccgctcagt tccggccggg
      181 gaccggcgag atgtcgccga tcagcccgtc cttctcgtcg tggatgccgg cggtgaagta
      241 cagatcctcg ggctcggccc ccttgaaccc gccgaacttc agcgcccaca gcccgtcgat
      301 cgtgatcgtc ttgccgttcc gggcgcgcag ctggccgagg aactcgccgt ggttgccgaa
      361 cgcgttcatc ttgccgtcgc cgaagttacc gaccaggatc gcaccgccga acttgccgaa
      421 gctgagcggc gcgcgcgcca ggccccacgg ggcgttgagc gtgccgcgcg tcgcgaaccg
      481 ccggaccagc ttgccgctgg tggtgaacac gtcgacgaag cccagcccgg gcccggcgac
      541 gtcgtcctcc ttcgccttgt cctgcttggc gaacgtcacg aacagctcgc cgcagatgtt
      601 ctcgatgccg aacggcgcaa aacccgacgg gatgtcgcga tcgatgaacg gaaacctggt
      661 gacctcggtg aagttcgtgt cataggcctc gaccttggcg gcgcggaagt tggtgacgaa
      721 gatgaagctg ccctccgggt tggtgccgaa cgccgcgccc ttgtacaccc cgcccgcgtt
      781 ggtcttgatc acggtcgcca gcgacaggcc caccgggtcc ggcgggttgg cgttcgcatt
      841 ccatccgacg atctgaccgc cctcgctgac cgcgatgaac gtcgccggga acttcgtgtt
      901 cgggaggaag aactggttgg ggctggggtt ccacaccagc ccggtcggcg cccaggtgtt
      961 gtcctgcggg ctgagcttca ccaccagctt gccgacctgg ggcatgtcct tggggttcag
     1021 cgagtacacc gtcaacgcgt ggctgccgtt gtccgccacc cacagcgcct cgcccggtat
     1081 gaacgcggcg ccccacgggt tgaccgtgtt gtgatctgtc accttggcca ggttcgggat
     1141 gttcgacacc aggttggtca cctgaaatgc gccctgccga tcctcgctcc gatcgtcgtc
     1201 gccgcgggcg acgccggcca cgaccgctgc tccgagcaat gccgcggcgc cgaccgcggt
     1261 ggtgattgcc agatgctccc tcatgatata cctctccttc cttcctgttg tttgattcac
     1321 gactctttcg cgaacatgag tgctcatgct gcgagatggc gtatccgaca cgtcatctgt
     1381 gtccggcagc ccggggtgcc ggtgcgcgcg tacgctcggg cggtgtgccc cgtcgcgcgg
     1441 ccgccgtcgc cgccgccgcc tcggtgatgt ccttggttcc gccgccgacc accgtctacc
     1501 atgctcgggc gtatcggaca gggaataatc acgatcggtg gactgtccgg tagcaggaca
     1561 ctcctatctc gcagatcacc gcattccaca ctgcagcacg ggtaagcagc gccacggtca
     1621 cgccgcccgg gcgtgcgcat gcgcccgcga tcgcggccgc gagatcgcca tccggtgtcg
     1681 agagatcgtg tgttaccggt ccctgccggc gaccgccgtg atttgccgga ccgacgtgtc
     1741 gcgaaccggt tccggaacgg tcgtgttccg gaacagagcc tgcccgggat cgccgtgaca
     1801 cgcgctgccg agccagcgga tcgacgaatc accgaaccgg acgtcgatcc tcgacgtcgc
     1861 gcgatcggcc gcgggcgccg cccgaggtcg cgcggcagtt tcccgaggtc ctgatcttcg
     1921 atgcccgcca cg
//
LOCUS       DASVOR010000556         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_531249,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000556 DASVOR010000000
VERSION     DASVOR010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531249"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..739
                     /gene="pth"
                     /locus_tag="VF516_11020"
     CDS             113..739
                     /gene="pth"
                     /locus_tag="VF516_11020"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX8108252.1"
                     /translation="MWLVVGLGNPGAKYARNRHNIGFQVVDELAAKHGLPAWKSGKLG
                     GETTAGVVTTERGRERCVLLKPMEYMNLSGFAVQRTADFHSVEPASILVVHDEIDLEL
                     GMVRLKSGGGHGGHNGLRSIMEQLGGGGFARLRMGVGRDPRVPPGADKQAVASWVLSD
                     FPRDQAGVVTAMIQAGCEDIATVLARGIGPAMNQHNARPKLSSPGSAA"
     gene            819..1352
                     /gene="rpsF"
                     /locus_tag="VF516_11025"
     CDS             819..1352
                     /gene="rpsF"
                     /locus_tag="VF516_11025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEX8108253.1"
                     /translation="MTRLASLRDKPGTAREYETIYILRPNTPNEGVSEVNTRIKGVIE
                     GMGGKIIKVDNWGKRRLAYEVAKERKGIYLYWLYLAQPGVVEETERNLRMLDSVIRYL
                     TVKVDEDVDVTARPIELDDTSFEKAAQTAADEEDLFLSRGSDEASSDDDDDEFGGDDF
                     DNVPAIEPNIVPGESKE"
     gene            1356..1646
                     /gene="rpsR"
                     /locus_tag="VF516_11030"
     CDS             1356..1646
                     /gene="rpsR"
                     /locus_tag="VF516_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEX8108254.1"
                     /translation="MTTRMDAPDEGLDRDGDDRRGGKGRGAGARRKLLSQEIVEKIDY
                     KNPQILRSFITDRGKMIPRRISGASARQQRVISTAIRRARMLALLPFSVTGD"
     gene            1650..2105
                     /gene="rplI"
                     /locus_tag="VF516_11035"
     CDS             1650..2105
                     /gene="rplI"
                     /locus_tag="VF516_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEX8108255.1"
                     /translation="MAMQVILTQDVDNLGKAGEVVSVRPGYGRNYLVPRGLAVSATVR
                     NKNRLTHEKALIERKVAKERATATDLAARLNGMTLQFERMVGEDEKLFGSVTNRDIAD
                     QLKRAGVEIDHRTIQLDQAVKALGKYEVPVKLTAGVTANLKFWVVGKDK"
     gene            2122..>2762
                     /locus_tag="VF516_11040"
     CDS             2122..>2762
                     /locus_tag="VF516_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108256.1"
                     /translation="MSRGWFASVLLLVPGTLAAASNPWGQPGPTPLQGAPGPTPEVSG
                     NPAPVALPAVPSFAVPAPVSGVHTPFELLAAGERLHGARLQVGGYITWIHDCLELPAA
                     DGSAARRDRDPPPCERPKLLLGDTPGASVDRSLWVVDLPHTLGGLEWHLPGGQLARLH
                     HLAVGDYVVVTGTLAIRSPHHEVNSAGLIVYEALAPATAPAAAPPVALAEPALP"
BASE COUNT          459 a         1006 c          941 g          356 t
ORIGIN      
        1 agcctctggg gccaaggacg ccaaggccgc cgcacctgcc aaggacgcca aggccgcgac
       61 cccggccaag gcggccccgg ctggcaagcc cgctgccaag aagtagcgcg ccgtgtggct
      121 cgtggtgggg ctgggcaacc cgggcgcgaa gtacgcccgc aaccgccaca acatcggctt
      181 ccaggtcgtc gacgagctcg ctgccaagca cggcctgccg gcgtggaagt ccggcaagct
      241 cggcggcgag accaccgccg gcgtggtcac caccgagcgc ggccgcgagc gctgcgtgct
      301 gctcaagccg atggagtaca tgaacctgtc ggggttcgcg gtgcagcgca ccgccgactt
      361 tcacagcgtc gagccggcgt ccatcctggt cgtccacgac gagatcgacc tcgagctcgg
      421 catggtccgg ctcaagagcg gcggcggcca cggcggacac aacggcctgc gctcgatcat
      481 ggagcagctc ggcggcggcg ggttcgcccg gctgcggatg ggcgtcggcc gcgacccccg
      541 cgtgccgccc ggcgccgaca agcaggcggt ggccagctgg gtgctgtccg actttccccg
      601 cgaccaggcc ggggtggtaa ccgccatgat ccaggcaggt tgcgaggaca tcgcgaccgt
      661 tttggcccgc ggtatcgggc cggcgatgaa ccagcacaac gcgcggccga aactctcgtc
      721 tcccgggtcg gcggcatgat atagaccccc gctcgcacat acctcggtcg ccaggctcgt
      781 cctcggactc ccggcggccc attgcccacg aggaacacat gacacgactc gcgtcgcttc
      841 gcgacaagcc gggcactgcc cgcgaatacg agaccatcta catcctgcgc ccgaacacgc
      901 ccaacgaggg cgtctccgag gtcaacaccc gcatcaaggg cgtgatcgag ggcatgggcg
      961 gcaagatcat caaggtcgac aactggggca agcgccggct cgcgtacgag gtcgccaagg
     1021 agcgcaaggg catctacctg tactggctgt acctcgccca gcccggcgtc gtcgaggaga
     1081 ccgagcgcaa cctgcgcatg ctcgactcgg tgatccgcta cctcacggtc aaggtcgacg
     1141 aggacgtcga cgtcaccgcg cggccgatcg agctcgacga cacctcgttc gagaaggcgg
     1201 cgcagaccgc ggccgacgag gaggacctgt tcctgtcgcg cggctcggac gaggcgtcgt
     1261 cggacgacga cgacgacgag ttcgggggcg acgacttcga caacgtgccc gcgatcgagc
     1321 cgaacatcgt tcccggcgag agcaaggagt aagccatgac cacccgtatg gacgcacccg
     1381 acgaaggcct cgatcgcgat ggcgacgatc ggcgcggcgg caagggccgc ggcgccggcg
     1441 cgcggcgcaa gctgctgtcc caggagatcg tcgagaagat cgattacaag aacccgcaga
     1501 tcctgcggtc gttcatcacc gaccgcggca agatgatccc gcgccggatc agcggggcga
     1561 gcgcgcgcca gcagcgcgtg atctccaccg cgatccgccg cgcgcggatg ctcgcgctcc
     1621 tgccgttctc ggtcaccgga gactgaccca tggcgatgca agtcatcctc acccaggacg
     1681 tcgacaacct cggcaaggcc ggcgaggtcg tctcggtccg ccccggctac ggccgcaatt
     1741 acctggtccc gcgcgggctc gcggtcagcg cgaccgtgcg caacaagaac cggctgacgc
     1801 acgagaaggc gctgatcgag cgcaaggtcg ccaaggagcg cgcgaccgcg accgacctgg
     1861 cggcgcggct caacggcatg acgctgcagt tcgagcgcat ggtcggcgag gacgagaagc
     1921 tgttcggctc ggtcaccaac cgcgacatcg ccgatcagct caagcgcgcc ggggtcgaga
     1981 tcgaccaccg cacgatccag ctcgatcagg cggtcaaggc gctcggcaag tacgaggtgc
     2041 cggtcaagct gacggccggc gtcaccgcga acctgaagtt ctgggtagtc ggcaaggaca
     2101 agtagccgat acactcacgc catgtcgcgt gggtggttcg ccagtgtgct gctgctcgtg
     2161 cccggcactc tcgccgcggc atccaacccc tgggggcagc ccggcccgac gccgctccag
     2221 ggagctcccg ggcccacgcc cgaggtcagc ggcaacccgg cgccggtcgc gctgcctgcg
     2281 gtgccgagct ttgccgtccc cgcgccggtc agcggcgtcc acacgccgtt cgagctgctc
     2341 gccgccggcg agcggttgca cggagcccgg ctccaggtcg gcggctacat cacctggatc
     2401 cacgattgcc tggagctccc cgcggccgac ggctcggccg cccggcgcga tcgcgacccg
     2461 ccgccgtgcg agcggcccaa gctcctgctc ggcgacaccc cgggcgcctc ggtggatcgc
     2521 agcctgtggg tcgtcgatct gccgcacacg ctgggcggac tggagtggca cctgcccggg
     2581 ggccagctcg cccggctcca ccacctcgcg gtcggcgact acgtcgtggt caccggcacg
     2641 ctggcgatcc gctcaccgca ccacgaggtc aactccgccg gcctgatcgt gtacgaggcg
     2701 ctcgcgcccg cgaccgcgcc cgcagccgcg ccgccggtgg cactcgccga gcccgcgctg
     2761 cc
//
LOCUS       DASVOR010000557         2312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_151251,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000557 DASVOR010000000
VERSION     DASVOR010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151251"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF516_11045"
     CDS             complement(<1..717)
                     /locus_tag="VF516_11045"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108257.1"
                     /translation="MDTADLLEERFEVEHQVRAGGMGAVFRARDRSSGELVAIKVIGN
                     GRDTHGARFAREVQLLSELSHPGIVRYIAHGVTPSGARFLVMEWLDGEDLWRRLDREP
                     LSIGESVALAARVAEALGAAHARGIIHRDLKPSNLFLPDRRIDQVKVLDFGIAHKAGM
                     TQLTRTGSMVGTPGYMAPEQARSHGAIDARADVFALGCVLFHCLTGTAPFEGDTAIAV
                     LGKILFDEAPRVSALWPEVPE"
     gene            complement(845..>2312)
                     /locus_tag="VF516_11050"
     CDS             complement(845..>2312)
                     /locus_tag="VF516_11050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108258.1"
                     /translation="EDRGQLFVLTRFDDAISVIDTRTRAETAHVAMHNPEPPSVTTGR
                     RFLYDASLSSAHGDSACASCHVFGDFDSLAWDIGNPDAPVTDIPGPFASALFNPFTTP
                     PTPLDPIFHPMKGPMTTQSLRGMLNSGPMHWRGDRTGGGPGVTSAQPDSGTFDERTAF
                     GKFQAGFTDLLGRDSFIPTADMDAFTDFILQVTYPPNPNRALDNSLTAEQETGRQLFS
                     GVNCGIPAPDGRLNVLTCISCHVIDPGGNPGTAAPGFFGTTGFYTFEGELQLFKVPHL
                     RNLYQKVGMFGNPRESGVLGDNFGFQGDQVRGFGFLHDGDIDTVFRFHHGPTFSVLLT
                     GEGNGGFPEGPEGELQRRQVESFVLVFPSNLAPIVGQQITLTADSPPAVAARIDLLRQ
                     RADAGECDLVAKTTLFDIEAGFLYAGGGRFRTDRRAAPPIGDAALRLLARTGHPVTFT
                     CVPPGSGERIGVDRDGDGAWDGDERDAHTDPADPTGRP"
BASE COUNT          330 a          758 c          877 g          347 t
ORIGIN      
        1 ctcgggcacc tcgggccaca gcgcgctgac ccggggcgcc tcgtcgaaca agatcttgcc
       61 gagcaccgcg atcgcggtgt ccccctcgaa cggcgccgtg ccggtcaggc agtggaacag
      121 cacgcagccg agcgcgaaca cgtcggcgcg cgcgtcgatc gcgccgtggc tgcgcgcctg
      181 ctcgggcgcc atgtagcccg gcgtgccgac catcgagccg gtccgggtca gctgggtcat
      241 ccccgccttg tgggcgatgc cgaagtcgag caccttgacc tgatcgatcc ggcgatcggg
      301 caggaacagg ttgctcggct tgagatcgcg gtggatgatg ccgcgggcgt gggcggcgcc
      361 gagcgcctcg gcgacccggg cggcgagcgc gaccgactcg ccgatcgaga gcggctcgcg
      421 gtcgagccgg cgccacaggt cctcgccgtc gagccactcc atgaccagga accgcgcgcc
      481 cgacggggtg acgccgtgtg cgatgtaccg gacgatgccg gggtgcgata gctcggacag
      541 cagctggacc tcgcgggcaa accgcgcgcc atgggtatcg cggccgttgc cgatgacctt
      601 gatggcgacg agctcccccg agctgcggtc gcgggcgcga aacaccgcgc ccatcccacc
      661 agcgcgaacc tggtgctcga cctcgaaccg ctcttccagc agatccgcgg tgtccatcgc
      721 cttggcggaa ttgtccgttg cctggaaggc agacaacaag accttgtttc ggagcccgta
      781 gtgaggactc agtgcgtgtg ctcgggcgtg atacccgatc gcgaccccac ggttgcttgt
      841 ctcgtcacgg ccgaccggtg gggtcggcgg gatcggtgtg tgcgtcgcgc tcgtcgccgt
      901 cccaggcgcc gtcgccgtcg cggtcgacgc cgatccgctc gccggagccg ggcgggacgc
      961 aggtgaaggt cacggggtgg ccggtacggg cgagcaggcg cagcgcggcg tccccgatcg
     1021 gcggcgcggc gcgccggtcg gtccggaacc ggccgccccc ggcgtagagg aagccggcct
     1081 cgatgtcgaa cagggtcgtc ttggcgacca gatcgcactc accggcgtcg gcgcgctggc
     1141 ggagcaggtc gatgcgggcg gccacggcgg gcgggctgtc cgcggtcagc gtgatctgct
     1201 ggccgacgat cggcgcgagg ttcgagggga acaccagcac gaacgactcg acctgccggc
     1261 gctggagctc gccttcgggg ccctcgggga acccgccgtt gccttcgccg gtcagcagca
     1321 ccgagaacgt cgggccgtgg tggaagcgga acacggtgtc gatatcgccg tcgtggagga
     1381 acccgaagcc gcgcacctgg tcgccctgga agccgaagtt atcgccgagc acgccgctct
     1441 cgcggggatt cccgaacatt ccgaccttct ggtacaggtt gcgcaggtgg gggaccttga
     1501 acagctggag ctctccctcg aaggtgtaga acccggtcgt gccgaagaac ccgggcgcgg
     1561 ccgtgccggg gttgccaccg gggtcgatca cgtggcagct gatgcaggtc aggacgttga
     1621 gccgtccgtc gggcgccggg atgccgcagt tgacgccgct gaacagctgg cgtccggtct
     1681 cctgctcggc ggtgagcgag ttgtcgagcg cccggttggg attcggcgga taggtgacct
     1741 ggaggatgaa gtcggtgaac gcgtccatgt cggcggtcgg gatgaacgag tcgcggccga
     1801 gcaggtcggt gaacccggcc tggaacttcc cgaacgcggt gcgctcgtcg aacgtgccgc
     1861 tgtcgggctg cgcgctcgtc acgccggggc cgccgccggt gcggtcgccg cgccagtgca
     1921 tcggtccgct gttgagcatg ccgcgcaggc tctgggtcgt catcggcccc ttcatcgggt
     1981 ggaagatcgg atcgagcggc gtgggcggtg tcgtgaacgg gttgaacagc gcgctggcga
     2041 acggcccggg gatgtcggtc accggcgcgt cgggattgcc gatgtcccag gccaggctgt
     2101 cgaagtcgcc gaacacgtgg cagctggcgc acgccgagtc gccgtgggcc gacgagagcg
     2161 acgcgtcgta caggaaccgg cggccggtcg tgacgctggg cggctcgggg ttgtgcatcg
     2221 cgacgtgcgc ggtctcggcg cgggtccggg tgtcgatcac cgagatcgca tcgtcgaacc
     2281 gggtgagcac gaacagctgg ccgcggtcct cg
//
LOCUS       DASVOR010000558        10938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238750,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000558 DASVOR010000000
VERSION     DASVOR010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10938
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238750"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="VF516_11055"
     CDS             complement(<1..341)
                     /locus_tag="VF516_11055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108259.1"
                     /translation="MRAYIELGASLDDVPTWSLRTLNTGSARSSPAYGRHLDATIGSS
                     YDWSDELRFDKHAGKLTSFVLKMPEAGALEPAIARSWLALPRRTGIPVLDHRKNGFHV
                     DPLDLRHLAEDA"
     gene            complement(439..1272)
                     /locus_tag="VF516_11060"
     CDS             complement(439..1272)
                     /locus_tag="VF516_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108260.1"
                     /translation="MTGPGVEPLADAGVALGRLAESLVISYELVPAAATFTLVADYPD
                     RAPGAQRSFVALRFSGVRGFHRDPGNLAELQAFTDTYSVADSPRPIVIQHLSITGDES
                     DRSVELSFGPNFGGVEFSYGAVAGFIRHARAEERDGDWIYRDARTGEAFDFARPFVSA
                     LPPTRSPGPRSPRRTIEMPDTIDLLARAKQYTPGDPEAAACWNLVVNAIDVGCLDPDE
                     MRELIEHLRDHRDFGVEELLFAHVGDKLRVSFLDDKATCSSSAMLAALIAVSGPESTG
                     D"
     gene            complement(1269..2141)
                     /locus_tag="VF516_11065"
     CDS             complement(1269..2141)
                     /locus_tag="VF516_11065"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8108261.1"
                     /translation="MQARALEGIAGSGQPLPYRARIQALFGRHDVSGVTAHVGDEAKQ
                     AADDIGADAYATGDRIAFSSSPDLHTAAHEAAHVVQQRGGVQLKDRVGRPGDAYERHA
                     DAVADRVAAGQSGEDLLDQMAGGSPTAAVQMQAATARESTTTVAGAPSGSAEPAGITT
                     DGAVAIRPNLMAATGAGGARAGSRAEPPFPRRTLRGVSLDWLRRNKPRGWREVPTRDN
                     EGWIWLDAAGRERLRFMRPNGENPSASQWSRQANGYFRWQDEAGNYLDIDGNVVPPSH
                     PQFNERTHIMYEGP"
     gene            complement(3184..3399)
                     /locus_tag="VF516_11070"
     CDS             complement(3184..3399)
                     /locus_tag="VF516_11070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108262.1"
                     /translation="MNAGPARSSPAYDRHLVGTIGSSYDWSDELRFDKRTGKLRSLVL
                     KTPEAGALEPAIARSWLALPRRTGISP"
     gene            complement(3658..4722)
                     /locus_tag="VF516_11075"
     CDS             complement(3658..4722)
                     /locus_tag="VF516_11075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8108263.1"
                     /translation="MLTEFIETHRALILARARERLLSRAIPRPTERELQHGVPIFLDQ
                     LSARLRSGLEADTAPIGTSASLHGGELQKLGFTIGQVVHGYGDVCQTITELAVERATQ
                     ISNEDFRTLNLCLDTAIAEAVTEYARQREHTIAEQGVEHLGFLAHELRNLLSAASLAF
                     ETAKSGRLDLSGRTGSLLSKSLVGMHDLVTRSLAEVRLESGAPRRERISLTAVLEEIE
                     ITATAQCRRRDVQLSIEPAAPDIKFEGDFQLVASIITNLIQNACKYTRAHGGVMLRTC
                     VVSDRVLIDVADECGGLPPGKADELFRPYEQRSSDRSGLGLGLAISLKAAKAMGGTIH
                     VRNVPGTGCVFTLDLTRAST"
     gene            4770..5138
                     /locus_tag="VF516_11080"
     CDS             4770..5138
                     /locus_tag="VF516_11080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108264.1"
                     /translation="MRAASLHPTVTNQKRHCSPERCAHGMFYRDSRTICENPSAGARR
                     WARMLSAVVLLIPAAPIVAVAVLAVLALVARGKQVRAAAGLPQVDARPDRYPLPLARA
                     RSIARGTALRAAELDLDMMV"
     gene            complement(5100..10898)
                     /locus_tag="VF516_11085"
     CDS             complement(5100..10898)
                     /locus_tag="VF516_11085"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX8108265.1"
                     /translation="MLAAWALAPLWSWWLSRPIAAAGPQLAAAERAFLRTVARRTWRF
                     FEVHVTAGDHFLPPDNVQDDPPRGAAHRTSPTNIGMSLIGNLAAYDFGYLSAGELIAR
                     TAQTFATLDRMQRYRGHFYNWYDTTTLEPLHPLYVSTVDSGNLAGHLLTLAGGFDELA
                     DRAARPTLTGLGDTLAVLAELAATWPEVVRELALLRSDLAGAVRTPAEAYAMLQRAAS
                     RAQDLMRVLDARGVAQRTAGGAVAPGPEVAPSAAEAIWWARAFDAQRSELVHELVLLA
                     PWVELPPAANPTVRALLDGPLTLGDTAGLDLASLAAGAPAVQGAVVRAVEFATERLGE
                     LRALALRCRELADLEYDFLYDRERHLLAIGFSVTDRRLDASFYDLLASEARLASFVAI
                     AQGKLPQDHWFSFGRQLTTSHGRPALLSWSGSMFEYLMPLLVMPTYGGTLLDETYRAV
                     VERQIAYGRERRVPWGVSESGYYKTDAQLNYQYRAFGVPGLGFKRGLADDLVIAPYAS
                     ALALMIAPRAACANLRELARSGLLGAFGFYEAVDYTAARMPTDKDHVIVRSVMAHHQG
                     MTLLAIAYALLGRPMQRRFAAAPSFRATELLLQEHVPRALAIFPHPAEVSSVTAGDAE
                     HDLRVFTTPDTPAPEVHLLSNGHSHVAITTAGGGYSRWRDLAVTRWHEDPTRDCWGAF
                     GYLRDVTSTAPAGGAAASACWSIAHHPTLARATSYEAIFSQGRAEFRRVDHDIDTHVE
                     ISISPEDDIELRRISLTNRGRMPRTIELTSYAEVVITHPAADAAHPAFSNLFVQTELV
                     RPHQAILCTRRPRSGDERPPWMIHVMMVHGTADGEPSYETSRAAFVGRGRSVIDPIAM
                     HQAALTDSCGAVLDPIVAIRTRVVLEPDETARFHVVTGMAETRDVALGLIEKYRDRHA
                     ADRVFELAWTHSQVVQRRLEATSADTQLYERLASNVLYANPTLRAPSSVLARNRGGQS
                     GLWAYGISGDLPIVLVRIADVAHIELVRQLVKAHAYWRLKGLSADLVIWNEDPSGYRQ
                     VLQDEIMSAIAASDSPLDRPGGIFVRRSEQISDEDKLLMQTVARLIVTGSAGTLAEQL
                     ERKPRAELPLLPASPRTLPVYDHAEPSAPSTAPALAPSLAAFARPDLVDFNGLGGFTT
                     DGREYVITTTGDRRTPAPWINVLANPWFGSIVSESGSAYTWCENAHGYRLTPWHNDAV
                     GDPSGEALYLRDEDDGHVWSPTPLPVTAAAPYISRHGFGYSVFEVSERGIASELHTYV
                     ATDAPIKFLVLKVRNRSGRQRRLSITGLFELVLGAARATSSPHIVTELDARTGGLFAR
                     NAYNSEFAARVAFLDCSEGQRTVTGDRLEVLGRNGSPVRPACMQRARLSGRVGAGLDP
                     CFAMRVMLELAEGQEREIAFTFGSGRDLADARHLVTRFRGTERARIALEGVWAYWNRT
                     LGAVHVQTPDHAVNFLVNGWLLYQVLACRLWARSGFYQSGGAYGFRDQLQDAMALVHA
                     EPAVLRDQLLRAASRQFPQGDVQHWWHPPLGRGVRTHISDDYLWLPYATCRYVHALGD
                     TGVLDEKVAFLDGRTVEQDEDSYYDLPARSDESASLYDHCVRAIRHGLRFGAHGLPLM
                     GSGDWNDGMNLVGELGKGESVWLAFFLHDVLVQFSALARRRGDTAFAETCTTEAARLR
                     GNIDEHAWDGAWYRRAYFDDGEPLGSAASPECQIDSLPQSWAVLSHAADPERARLALD
                     AVDRRLVRRDLGVIQLLDPPFDTSALKPGYIKGYVPGVRENGGQYTHAAVWAAMAFAA
                     AGDAARAWELFDLINPLHHGNSEAAIAVYKVEPYVVAADVYTNPQHAGRGGWTWYTGS
                     AGWMYQLVTESLLGIRLEVDQLRIEPRLPRAWRDLTVHYRHRETVYHLQVKNLGGTLS
                     RVVCDGIEQANHRIPLHDDRLDHHVEIELGGA"
BASE COUNT         1631 a         3724 c         3845 g         1738 t
ORIGIN      
        1 gcgtcctcgg cgagatgtcg caggtccagc ggatcgacgt ggaagccgtt tttgcggtgg
       61 tcgagcaccg ggatcccggt ccgtcggggt agggcgagcc aagatcgcgc gatcgccggc
      121 tcgagtgccc cggcctccgg catcttgagc acgaagctcg tcagcttgcc ggcgtgcttg
      181 tcgaaccgga gctcgtcgct ccagtcgtac gagctgccga tcgtggcgtc caggtgccgg
      241 ccgtacgctg gcgaggatcg cgcagagcct gtattcagcg tccggagcga ccacgtcgga
      301 acatcgtcga ggctcgcgcc gagttcgatg taagccctca cctcatcaat gggggttcga
      361 cgtcgggaac gtgacggcca tcgagatggg atgctcggtc gcggtcatgg tgccctcgct
      421 acgagcacct gtggcagctc agtcaccagt gctctctggc ccagagacgg cgatgagcgc
      481 cgcgagcatc gccgaggatg aacacgtcgc cttgtcatcg aggaacgaga cgcgtagctt
      541 gtcaccgaca tgggcgaata gcagctcttc gacgccgaag tcgcggtgat ctcgcaagtg
      601 ctcgatgagc tcacgcattt cgtccgggtc gaggcatccc acatcgatcg cgttcacgac
      661 gaggttccag cacgcggcgg cctcgggatc accaggagtg tactgttttg cccgggcgag
      721 gagatcgatc gtatctggca tttcaatcgt ccttcggggc gaccttgggc caggacttct
      781 cgtaggcggg agcgcgctta cgaacggccg cgcgaagtcg aatgcctcgc cggtgcgcgc
      841 gtctcggtag atccagtcac cgtcgcgctc ttcggcgcgg gcgtggcgga tgaaccctgc
      901 caccgcgcca taggagaact cgaccccgcc gaagttgggt ccgaacgaca gctccacgga
      961 tcggtcgctt tcgtcgccgg tgatgctcag gtgctggatg acgatcgggc gcgggctgtc
     1021 cgcgacggag tacgtgtccg tgaacgcctg cagctcggcg aggttgcccg gatcgcggtg
     1081 gaagccacgc acgcccgaga accgcagggc gacgaatgag cgctgtgctc caggggcccg
     1141 gtcgggataa tccgcgacga gcgtgaacgt cgccgccgcc gggacgagct cgtacgagat
     1201 gaccaggctt tcggcaaggc ggccgagcgc gaccccggcg tcggcgagcg gttctacgcc
     1261 gggtccggtc acggcccctc gtacatgatg tgggtgcgct cgttgaactg agggtggctg
     1321 ggcgggacga cgttaccgtc gatgtcgagg tagttgcctg cctcgtcctg ccagcggaag
     1381 tagccgttgg cctggcgaga ccactggctg gccgacgggt tctcgccgtt ggggcgcatg
     1441 aagcgcagcc gctcgcgtcc ggcggcgtcg agccagatcc agccctcgtt gtcgcgggtc
     1501 gggacctcgc gccagccgcg gggcttgttg cgccgcagcc agtccagcga gacgccgcgc
     1561 agcgtccggc gcggaaacgg cggctcggcc cgcgacccgg cccgcgcacc gccggcaccg
     1621 gtcgccgcca tcaggttggg ccggatcgcg accgcaccgt cggtggtgat ccccgcgggc
     1681 tccgctgatc cactcggcgc gcctgccacc gtcgtggttg actcccgcgc ggttgccgcc
     1741 tgcatctgga cggcagcggt cggcgagccg cccgccatct ggtcgagcag atcctctccg
     1801 ctctggccgg cggcgactcg atcggcgacc gcgtcggcat gtcgctcgta cgcgtcaccg
     1861 gggcgtccca cgcggtcctt cagctggaca ccgccacgct gctggacgac gtgcgccgct
     1921 tcgtgtgccg cggtgtggag atccggcgag ctcgagaacg cgatgcggtc gccggtcgcg
     1981 taggcatcgg cgccgatgtc gtcggccgcc tgcttcgcct cgtcgccgac gtgcgcggtc
     2041 acgccgctga cgtcgtggcg gccgaacagt gcctggatcc gtgccctgta cggcagcggc
     2101 tggccgctac cggcgatccc ctcgagcgca cgcgcttgca ccgctggtct cccgaccgct
     2161 ggcggatccc cgggcacggc ttccgcgccg tacggcattc cgatcggacc tctggtcagg
     2221 gtgaaacgac caggggctgc gccgaccgcg cgctccggcc cgtgagacgt agcgtccatg
     2281 gccccggtca gggtgaggcg accaggggcc gcgatcgttg tgcctttcgc ctcgcggtga
     2341 tcacgctgcc catcgggacc tcgcgtcttg cggtcgtaca ccattgtctc gactcgcggc
     2401 gggggcaccg cagccagagc ctaggacccg gtccaggcgg cggccaggat gtaagccgcg
     2461 tctcctgtgg ccagggcgag cgagttctcg ctgaaggtgg ggcagatcga gcgcctgtgc
     2521 cccgatgcct cttcgtgctg aggcggtcgc gctgtagctc cggcgcgttg gtatcgtccc
     2581 gcctcccagg accgctcgtg cagccatgcg ccatcctacc caccccaccg catcagttgt
     2641 ccgtcgggtc gacgctctcg tcggatctcg gctggcgttg acccacccgt gtcccacgtc
     2701 cccggctcgt tcgaggggaa actgaacgaa gacggggcct tgggcgagct gggcacgggc
     2761 ctgaaaatcc gcgtgtcgtt ggttcaattc cgaccctggg cacccgcttc gctggatctt
     2821 ctctcttcgg cgtcggagaa acagcggttt cggccgggcc cggcctcgcg gcgctcgcgc
     2881 caggagcgcc atccgcgcca cgatttcccg ggacgtttgt cccatatttg tcccacggcc
     2941 cggctcgttg cgcagaccgg tgcggcgaac tatgaccgcc ctcgggccgg tgcccgcgat
     3001 gctcacagcc gacctccgcg agcaggcgcg cgagcacggc gtcctggtgt ggctggacaa
     3061 ggaggggcgc ctacaccgcg tttgccgatc gatgcgtgat cacggcgtca cggaggcgtt
     3121 tccgatcccg gtgcgctgcc tgcgcggcag ctgctgcgcg actcgtacgt acgcagtacc
     3181 ccttcagggc gagatcccgg tccgtcgggg tagggcgagc caggatcgtg cgatcgccgg
     3241 ttcgagtgcc ccggcctccg gcgtcttgag caccaagctc ctcagcttgc cggtgcgctt
     3301 gtcgaaccgg agctcgtcgc tccagtcgta cgagctgccg atcgtgccaa ccaggtgccg
     3361 gtcgtacgct ggcgaagacc gcgcaggacc tgcgttcaac gtctggagcg accacgtcgg
     3421 aacatcgtcg aggctcgcac caagctcgac gtgagctgcc tcttcgccat cggggttcgg
     3481 gggtcgcgtt gcatctggtg aaacctgtcg ataccttgag gatcctcgac ggcctccgac
     3541 aggtcgccga ggccttcctt cgaggtcgag ctcagtggat ccggctccat gaccgtacgg
     3601 ctcgtcgcgt ctgcggaccg cgagacccgg cgatgatcac agcactcagc ggtcgtgtca
     3661 ggtcgatgcc ctggtgagat ccagtgtgaa cacgcatcct gtgcctggaa cattgcggac
     3721 gtgaatggtg ccgcccatcg cctttgcagc cttgaggctg atcgcaagcc cgagtcccag
     3781 accgctgcga tcggagctgc gctgctcgta ggggcggaac agctcatcgg ccttgccggg
     3841 tggtagccca ccacattcat cagccacgtc gatcaacact cgatcggaga ccacacatgt
     3901 ccgcaacatg acaccgccgt gcgcgcgagt atacttgcaa gcgttctgta tcaggttggt
     3961 gatgatcgat gcgacgagct ggaagtcgcc ctcgaacttg atgtcgggcg ccgccggctc
     4021 gatcgagagc tggacgtcgc ggcgcctgca ttgtgcggtc gcagtgatct cgatttcctc
     4081 caggacggca gtcagcgaga tccgttcgcg acggggcgcg ccggactcga gccgcacctc
     4141 ggcaagcgag cgagtcacga gatcgtgcat gccgacaaga cttttgctca gcaggcttcc
     4201 cgtgcgtcca ctgaggtcaa gccgtcccga tttcgccgtt tcgaacgcaa gcgaggccgc
     4261 gctgaggagg tttcgtagct catgggccaa gaaacccaga tgctcgaccc cctgctccgc
     4321 gatcgtatgc tctcgctgcc gcgcgtactc ggtgacggcc tcggcgatcg ctgtgtccag
     4381 acacaaattc aacgtacgaa agtcttcatt cgagatctgc gttgcacgct cgacggcgag
     4441 ctcggtgatc gtctgacaga catcgccata accgtgaacg acctggccga tcgtgaatcc
     4501 gagtttctgg agttcaccgc cgtgaagcga cgcactggtg ccgatcggag ccgtgtctgc
     4561 ttccagcccc gaacgtaatc gagccgagag ctgatcgagg aagatcggca cgccatgctg
     4621 gagctcgcgc tccgtcgggc gtggtatcgc gcggctcagc agccgctcgc gcgctcgcgc
     4681 gaggatcagc gcacgatgag tctcgatgaa ctcggtcaac atgtcgaaac catgccatgc
     4741 gctcgtctca tgcctccccg cgacgggtca tgcgtgcggc gtcgctgcac cccacggtga
     4801 cgaatcagaa aaggcattgc tcgccggaac gctgcgcgca tggtatgttt tatcgcgatt
     4861 cgagaacaat ctgcgagaat ccttcggccg gcgcacgacg ctgggctcgg atgctttcag
     4921 ccgtcgtgct gctcatcccg gccgctccga tcgtcgccgt tgcggtgctc gcggtgctcg
     4981 cgctggttgc gcgcggcaag caggtccggg ctgccgccgg tctgccccag gtcgacgcgc
     5041 ggcctgatcg atacccgctg cctctcgcgc gcgcccggtc gatcgctcga ggcaccgcgc
     5101 tacgcgccgc cgagctcgat ctcgacatga tggtctagcc ggtcgtcgtg cagcgggatc
     5161 cggtggttgg cttgctcgat gccatcgcac accacgcggc tgagggtgcc gccgaggttc
     5221 ttgacctgga ggtgatagac cgtctcgcga tggcggtagt gcacggtcag gtcgcgccag
     5281 gcccgaggca ggcgcggctc gatccgtagc tggtcgacct cgagccggat gccgagcagc
     5341 gactcggtga cgagctggta catccagccg gccgagccgg tgtaccaggt ccagccaccg
     5401 cgcccggcgt gctgcgggtt ggtgtagacg tctgccgcga cgacgtaggg ctcgaccttg
     5461 tacaccgcga tcgcggcctc gctgttgccg tggtggagcg ggttgatgag atcgaacagc
     5521 tcccaggcgc gcgccgcgtc gccggccgca gcaaacgcca tcgcggccca caccgcggcg
     5581 tgggtgtact ggccgccgtt ctcgcggacg ccgggcacgt agcccttgat gtagcccggc
     5641 ttcagggcgg aggtgtcaaa cggcgggtcg agcagctgga tgacgccgag gtcgcggcga
     5701 acgaggcggc ggtcgacggc gtcgagcgcg agtctggcgc gctcgggatc ggctgcgtgg
     5761 gacagcacgg cccagctctg gggcagcgag tcgatctgac actcggggct cgcggccgag
     5821 ccgagcggtt cgccatcgtc gaagtaggcg cggcgatacc acgcgccgtc ccaggcgtgc
     5881 tcgtcgatgt tgccgcgcag acgcgccgcc tcggtcgtgc aggtctcggc gaacgccgtg
     5941 tcgccgcggc gccgcgcgag cgcggagaac tgcaccagca cgtcatgaag gaagaacgcg
     6001 agccacacgc tctcgccctt gccgagctcg ccgaccaggt tcatcccgtc gttccagtca
     6061 ccgctgccca tcagcggcag gccgtgggcg ccgaaccgga ggccgtgcct gatcgcgcgc
     6121 acgcagtggt cgtagagcga cgccgactcg tcggaccgcg ccggcaggtc gtagtagctg
     6181 tcctcgtcct gctcgaccgt ccggccgtcg aggaacgcga ccttctcatc gagcacgccg
     6241 gtgtcgccga gcgcgtgcac gtagcggcag gtcgcgtacg gcaaccacag gtagtcgtcg
     6301 gagatgtggg tgcgcacccc gcggccgagc ggcgggtgcc accagtgctg gacgtcgccc
     6361 tgcgggaact ggcggctcgc cgcgcgcagg agctgatcgc gcaggaccgc cggctcggcg
     6421 tggaccagcg ccatcgcatc ctggagctgg tcgcggaacc cgtacgcgcc gccggactga
     6481 tagaacccgc tgcgcgccca cagccggcac gcgagcacct gatacagcag ccagccgttg
     6541 accaggaagt tcaccgcgtg gtcgggggtc tgcacgtgga ccgcgccgag cgtgcggttc
     6601 cagtaggccc acacgccctc gagggcgatc cgcgcccgct cggtcccgcg aaaccgggtg
     6661 accaggtggc gggcgtccgc cagatcgcgg ccggagccga acgtgaacgc gatctcgcgc
     6721 tcctggcctt ccgcgagctc cagcatcacg cgcatcgcga agcacggatc gagtccggcc
     6781 ccgacccggc cggatagccg cgcgcgctgc atgcaggccg gacgcacggg gctaccgttg
     6841 cggccgagca cctcgaggcg atcgccggtg accgtgcgct ggccctcgct gcaatcgagg
     6901 aacgcgaccc gcgccgcgaa ctcgctgttg taggcgttgc gcgcgaacaa gccgccggtc
     6961 ctggcgtcga gctcggtcac gatgtgcggc gagctggtcg cgcgtgcggc gccgagcacc
     7021 agctcgaaca gcccggtgat cgacagccgg cgctgcctgc ccgagcggtt acggaccttg
     7081 agcaccagga acttgatcgg cgcgtcggtc gcgacgtagg tgtgcagctc gctcgcgatg
     7141 ccgcgctcgc tgacctcgaa cacgctgtag ccgaacccat ggcggctgat gtagggcgcc
     7201 gcggcggtga ccggcagcgg cgtcggcgac cacacgtggc cgtcgtcctc gtcgcgcagg
     7261 tacagcgcct cgccgctcgg gtcgccgacc gcgtcgttgt gccacggcgt cagccggtag
     7321 ccgtgcgcgt tctcgcacca ggtgtacgcg ctgccgctct cgctcacgat gctgccgaac
     7381 cacgggttcg cgagcacgtt gatccacggc gccggcgtgc gccgatcgcc ggtggtcgtg
     7441 atgacgtact cgcggccgtc ggtcgtgaac ccaccgaggc cgttgaagtc gaccagatcg
     7501 gggcgggcga acgccgcgag gctcggcgca agcgccggtg cagtgctcgg cgcggacggc
     7561 tcggcgtggt cgtacaccgg gagggtgcgc gggctcgccg gcaggagcgg cagctcggcg
     7621 cgcggcttgc gctcgagctg ctctgccagc gtcccggcgg agccggtgac gatcagccgg
     7681 gcgaccgtct gcatcagcag cttgtcctcg tcggagatct gctcgctgcg ccgcacgaag
     7741 atcccaccgg gcctgtcgag cggactatcg ctcgccgcga tcgccgacat gatctcgtct
     7801 tgcaggacct ggcgatagcc cgacggatct tcgttccaga tcacgaggtc ggcgctgagg
     7861 cccttgagtc gccagtaggc gtgggccttg acgagctggc gcacgagctc gatgtgcgcg
     7921 acatctgcga tccggaccag cacgatcggc aggtcgccgg agatcccgta cgcccatagc
     7981 ccggattgcc cgccgcggtt gcgtgcgagc acgctgctgg gcgcacgcag cgtcgggttc
     8041 gcgtagagca cgttgctcgc gagccgctcg tacagctggg tgtctgcgct ggtcgcttcg
     8101 agccggcgct gcacgacctg gctgtgggtc cacgccagct cgaacacgcg atccgcggcg
     8161 tgacgatcgc gatacttctc gatcaagccg agcgcgacgt cgcgggtctc cgccatcccg
     8221 gtgaccacgt ggaaccgcgc ggtctcgtcc ggctcgagca cgacgcgggt acggatcgcg
     8281 acgatcggat cgagcaccgc gccgcagctg tcggtcagcg ccgcctggtg catcgcgatc
     8341 ggatcgatca ccgagcgacc acggccgacg aatgccgccc gcgaggtctc gtaggacggc
     8401 tcgccgtcgg cggtaccgtg caccatcatc acgtggatca tccacggtgg tcgctcgtcc
     8461 cccgaccgcg gccgccgcgt gcacaggatc gcctggtgag ggcggacgag ctcggtctgc
     8521 acgaacaggt tggagaacgc cggatgcgct gcgtcggcgg cgggatgggt gatcacgacc
     8581 tcggcgtagc tcgtcagctc gatcgtgcgg ggcatccggc cacggttggt caggctgatc
     8641 cggcgcagct cgatgtcgtc ctcgggcgag atgctgatct cgacgtgggt gtcgatgtcg
     8701 tgatcgacgc gccggaactc cgcgcggcct tgcgagaaga tcgcctcgta gctggtggcg
     8761 cgggcgagcg tcggatggtg cgcgatcgac cagcacgcag acgccgcagc accgccagcc
     8821 ggtgcggtgg acgtgacgtc gcgaaggtaa ccgaacgcgc cccagcaatc gcgggtcgga
     8881 tcctcgtgcc agcgcgtcac ggcgagatct cgccaccggc tgtagccacc gcccgcggtg
     8941 gtgatcgcca cgtgggagtg cccgttggac aagaggtgga cctcgggcgc cggcgtgtcc
     9001 ggcgtggtga acacgcgcag gtcgtgctcg gcgtcgccgg cggtgaccga cgagacctcg
     9061 gcggggtgcg gaaagatcgc gagggcgcgc ggtacgtgct cctggagcag cagctcggtc
     9121 gcgcgaaagc tcggcgcggc ggcgaaccgg cgctgcatcg ggcggccgag caacgcgtac
     9181 gcgatcgcga gtagcgtcat gccctggtgg tgcgccatga cggagcgcac gatgacgtga
     9241 tccttgtcgg tcggcatgcg cgcggccgtg tagtccaccg cctcgtagaa cccgaacgcg
     9301 ccgagcaggc cgctgcgcgc cagctcgcgc aagttcgcgc acgcggcccg cggtgcaatc
     9361 atgagagcga gcgcgctcgc gtacggcgcg atcaccagat cgtcggcgag cccgcgcttg
     9421 aaccccaggc cgggcacgcc gaacgcgcga tactgataat tgagctgcgc gtccgtcttg
     9481 taatagccgg actccgacac accccacggc acgcggcgct cgcgcccgta cgcgatctgg
     9541 cgctcgacga ccgcgcggta ggtctcgtcg agcagcgtgc cgccgtaggt cggcatcacg
     9601 agcagcggca tcaggtactc gaacatcgag ccgctccacg acagcagcgc cggcctgccg
     9661 tgcgacgtcg tgagctggcg gccgaagctg aaccagtggt cctgcggcag cttgccctgg
     9721 gcgatcgcga cgaagcttgc gagccgcgcc tcggaggcga gcagatcgta gaagctcgcg
     9781 tcgagccgac gatccgtgac gctgaagccg atcgcgagca ggtggcgctc gcggtcgtag
     9841 aggaagtcgt actccagatc ggcgagctcg cggcagcgca gcgcgagcgc gcggagctcg
     9901 ccgagccgct cggtggcgaa ctcgaccgcg cggaccaccg cgccctgcac ggccggcgcg
     9961 ccggcggcga gactggccag atcgaggccc gcggtatcgc cgagcgtgag cggcccatcg
    10021 agcagcgccc ggaccgtggg gtttgcggcc ggcggtagct cgacccacgg ggcgagcagg
    10081 acgagctcgt ggacgagctc ggaacgctgg gcgtcgaacg cacgcgccca ccagatcgcc
    10141 tcggcagcgc tcggcgcgac ctccggaccg ggtgccacgg cgccgcccgc ggtccgctgt
    10201 gccacgcctc gcgcgtcgag cacgcgcatc agatcctgtg cccgggacgc ggcgcgctgg
    10261 agcatggcgt acgcctccgc gggggttcgc accgcgccgg cgagatcgct gcgcaggagc
    10321 gccagctcgc gcacgacctc gggccaggtc gccgcgagct ccgcgagtac tgccagcgtg
    10381 tcgcccagcc cggtcagcgt cggtcgcgcg gcgcggtcgg ccagctcgtc gaaccctccg
    10441 gcgagcgtca acagatgacc ggccaagttg ccgctgtcga ccgtcgacac gtagagcggg
    10501 tgcagcggct cgagcgtcgt cgtgtcgtac cagttgtaga agtggccgcg gtaacgctgc
    10561 atgcgatcga gcgtggcgaa cgtctgcgcc gtccgcgcga tcagctcgcc ggcggacaga
    10621 taaccgaagt catacgccgc gaggttgccg atcagcgaca tcccgatgtt cgtcggcgag
    10681 gtccgatgcg ccgcgccgcg cggcggatcg tcctgcacgt tgtcgggcgg caggaagtgg
    10741 tcgccagcgg tgacgtggac ctcgaagaac cgccaggtcc ggcgtgcgac cgtgcgcagg
    10801 aatgcgcgct ccgctgcagc gagctgcggc ccggccgcgg cgatcggccg gctcagccac
    10861 cacgaccaca gcggtgcgag cgcccatgcg gcgagcaacg gcgccgcggc gatcgcgctc
    10921 ccgggctcga gccagcac
//
LOCUS       DASVOR010000559         4515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238755,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000559 DASVOR010000000
VERSION     DASVOR010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238755"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..698
                     /locus_tag="VF516_11090"
     CDS             147..698
                     /locus_tag="VF516_11090"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8108266.1"
                     /translation="MSRDTFCSYCGTRFADTTTYPRMCTACGAQTWANPIPVAVVLVP
                     VIDGARTGLLAVRRAIPPAGKLALVGGFVEEHESWQACVARELREETGVAIDPAALVP
                     LWYASSAPRPDRVLLFSIAPAIDASALPPFLPSAEVSQRGIWFGAGTTGGPDDELAFP
                     LHAEAARRYFAQASSHGSGFVAR"
     gene            complement(722..1393)
                     /locus_tag="VF516_11095"
     CDS             complement(722..1393)
                     /locus_tag="VF516_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8108267.1"
                     /translation="MGMKVILFGATGMVGQGVLRECLLAPDVESVLTVGRTATGQRHV
                     KLREIVHTDLTDYAAIEGDLAGHDACFFCLGVSAAGMSEQDYTRITYDVTLAAAQVLA
                     RLSPGMTFLYVSGEGTDSTERKRAMWARVKGRTENALRKLPLRAVMFRPGLIRPLHGI
                     RSKTRLYRMAYAALWPLFPVLGALGLMTTTERVGRAMLEVVRRGAPRPILTNRDIDEL
                     ARRSA"
     gene            1535..3880
                     /locus_tag="VF516_11100"
     CDS             1535..3880
                     /locus_tag="VF516_11100"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX8108268.1"
                     /translation="MHGDDELEAIDAYWRAANYLTVGQIYLMANPLLREPLRPEHIKP
                     RLLGHWGTSPGLNLIYAHLNRVIRHRDVNAMLLVGPGHGGPAIVANVYLEGTYSEIYP
                     AVSQDERGLDRLFRQFSTPGGVPSHVGPQTPGSIHEGGELGYVLTHAFGAVFDNPDLL
                     AVAVVGDGEAETGPLEGSWKGVKFLSAARDGAVLPILHLNGYKIAGPTVLGRADDTAI
                     RRLFEGHGYEVHLVAGHEPRAVHRQLASALDACLDRIRSLQSKARTRGARGAVRWPMI
                     ALRTPKGWTGPRVVNGLPVEGTFRAHQVPLADVRRNPEQLAMLDAWMRSYAPERLFDR
                     GGRLIASLRALAPAGARRMGANPHANGGALTRPLDLPPITDHVLDVPAPGTERRESTR
                     ALGALLRDVYRRNPTSFRLMCPDETTSNRLGAVFEVENRCFEEPALPTDDHVSSEGRV
                     MEVLSEHNCQGWLEGYVLTGRHGLFATYEAFALIVASMATQHSKWLEATAALPWRAPV
                     PSLNYLLTSTCWRNDHNGFSHQGPGFMDTIISKKGTVARVYLPPDGNCLLSVADHCLR
                     SKSYVNLVIIDKQPQLQWLAMPDARAHCARGLSIWDWAGTEAGHAPDVVLACAGDVPT
                     LETVAAAAWLRTHAPDLRVRVVNVVDLMRLFSPREHPHGMPDELFVDMFTRDVDVCFS
                     FHGYPGAIHQLLHARPNPPRFHVRGYKEEGTTTTPFDMVVLNETSRYHIAIDAIRRAR
                     RPPPNAAELTAECRRLLDEHRAYVATHFEDLPEIRDWTWRP"
     gene            3912..4229
                     /locus_tag="VF516_11105"
     CDS             3912..4229
                     /locus_tag="VF516_11105"
                     /inference="COORDINATES: protein motif:HMM:NF021516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2243 domain-containing protein"
                     /protein_id="HEX8108269.1"
                     /translation="MVYLMNLAMTVTGVALLFRAARRGATMSRRVLVGAMLGGWGLLT
                     VVEGMIDHQLLGAHHTHSGPDQLAWDVVYLMFGAALMLIGAAIACSRSEATCPPCAPT
                     TPS"
     gene            complement(4261..>4515)
                     /locus_tag="VF516_11110"
     CDS             complement(4261..>4515)
                     /locus_tag="VF516_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108270.1"
                     /translation="RQLPRALRDAGLCDVAADAYFPVALPASAALERRTIEHVRDRLI
                     ASGRVTADEIQRHFASVDAGELDLAMSPMISAWGRRPAAG"
BASE COUNT          622 a         1721 c         1550 g          622 t
ORIGIN      
        1 ccggtcggcc tcgcgatgat gccggacggc tcgctgctga tcggcgacga ccagggcggc
       61 cggatctggc gcgtgttcca tcccgggacg tgatcgtcgc ggcgcgattg acgcccggat
      121 ccgcccgctg cacgcttgcc cgacccatgt cccgcgacac gttctgctcg tactgcggca
      181 cccggttcgc cgacaccacg acctaccccc gcatgtgcac cgcgtgcggc gcgcagacct
      241 gggccaaccc gatccccgtc gcggtcgtcc tcgtgcccgt gatcgacggc gcgcgcaccg
      301 gcctgctcgc ggtgcgccgg gcgatcccgc cggccggcaa gctcgcgctg gtcggcggtt
      361 tcgtcgagga gcacgagtcg tggcaagcgt gcgtggcgcg cgagctgcgc gaggagaccg
      421 gcgtcgcgat cgaccccgcg gccctcgtgc cgctgtggta cgcgtcgagc gcgccccgcc
      481 ccgaccgcgt gctgctgttc tcgatcgcgc cggcgatcga cgcctcggcg ctgccgccgt
      541 tcctccccag cgcggaggtc tcgcagcgcg gcatctggtt cggcgccgga accaccggcg
      601 gccccgacga cgagctcgcc ttcccgctcc acgccgaggc cgcccggcgc tacttcgcgc
      661 aggccagcag ccacggctcc ggcttcgtcg cccggtgatc agcgccgtcc gggccgccgt
      721 ttcacgccga gcgccgcgcg agctcgtcga tgtcgcggtt ggtgaggatc ggtctgggcg
      781 cgccgcgccg cacgacctcg agcatcgccc ggccgactcg ctcggtcgtg gtcatcaggc
      841 cgagcgcgcc gagcaccgga aacagcggcc acagcgcggc gtaggccatg cggtacagcc
      901 gggtcttcga ccggatgccg tgcagcggcc ggatcagccc gggccggaac atcaccgccc
      961 gcagcgggag cttccgcaac gcgttctcgg tgcggccctt gacccgcgcc cacatcgcgc
     1021 gcttgcgctc ggtgctgtcg gtgccctcgc ccgacacgta gaggaaggtc atccccggcg
     1081 acagccgcgc gagcacctgg gccgccgcca gcgtgacgtc gtaggtgatc cgcgtgtagt
     1141 cctgctcgga catcccggcc gccgacacgc cgaggcagaa gaaacaggcg tcgtggcccg
     1201 cgagatcgcc ctcgatcgcc gcgtagtccg tcaagtcggt gtgcacgatc tcgcgcagct
     1261 tgacgtggcg ctgcccggtg gcggtccgac cgacggtgag caccgactcg acatccggag
     1321 cgagcaggca ttctcgcagc acgccctggc cgaccattcc agtggcaccg aacaagatga
     1381 ccttcatgcc catgcggccg agtgtgccct ggaacgggca cgcgcgtccc ggcgctcggg
     1441 gcgcgatgcg cgccctcgcc cggtgccgcc gcgcacgagc gcacgcccaa ccttacatcg
     1501 tcgcgctcgg ccgcgtggac tacagtcgca gcgtatgcac ggcgatgacg agctcgaggc
     1561 catcgatgcg tactggcgcg ccgcgaacta cctcaccgtg gggcagatct acctgatggc
     1621 gaatccgctg ctgcgcgagc cgctgcggcc ggagcacatc aagcccaggc tgctcggcca
     1681 ctgggggacg tcgcccggac tcaacctgat ctacgcacac ctcaatcgcg tgatccgcca
     1741 tcgcgacgtc aatgcgatgc tgctcgtcgg acccggccac ggcgggccgg cgatcgtggc
     1801 caatgtctat ctcgaaggta cgtattccga gatctacccc gcggtgtccc aggacgagcg
     1861 cgggctcgac cggctgttcc ggcagttctc gaccccgggc ggcgtgccga gccacgtcgg
     1921 gccgcagacc ccgggctcga tccacgaggg cggcgagctc ggctacgtcc tgacccacgc
     1981 gttcggcgcg gtgttcgaca atcccgatct gctcgcggtc gcggtggtcg gcgacggtga
     2041 ggcggagacc ggcccgctcg agggatcgtg gaagggcgtc aagttcctga gcgcggcgcg
     2101 cgacggcgcg gtgctgccga tcctgcacct caacggctac aagatcgccg gcccgaccgt
     2161 gctcggccgc gccgacgaca ccgcgatccg ccggctgttc gagggccacg gctacgaggt
     2221 ccacctggtc gccggccacg agccgcgcgc ggtgcaccgc cagctggcga gcgcgctcga
     2281 cgcctgcctc gaccggatcc gctcgctgca gagcaaggcg cggacccgcg gcgcgcgcgg
     2341 cgcggtgcgc tggccgatga tcgcgctgcg cacgcccaag ggctggaccg gccccagggt
     2401 ggtcaacggc ctgccggtcg agggcacgtt ccgcgcccac caggtcccgc tcgccgacgt
     2461 gcgcagaaac cccgagcagc tcgcgatgct ggacgcgtgg atgcggagct atgcgcccga
     2521 gcggctgttc gaccgcggcg gccggctgat cgcgtcgctc cgcgcgctgg caccggcggg
     2581 cgcacgccgg atgggcgcca acccgcacgc caacggcggc gcgctgaccc ggccgctcga
     2641 tctaccgccg atcaccgacc acgtgctcga cgtgccggcg ccgggcaccg agcgccgcga
     2701 gtcgacccgc gcgctcggcg cgctgctgcg cgacgtctac cggcgcaacc cgacgagctt
     2761 ccgcctgatg tgccccgacg agaccacgtc gaaccggctc ggcgcggtgt tcgaggtcga
     2821 gaaccgctgc ttcgaggagc ccgcgctgcc gaccgacgat cacgtgtcca gcgaaggccg
     2881 ggtcatggag gtgctgagcg agcacaactg ccagggctgg ctcgagggct acgtgctgac
     2941 cggccggcac ggcctgttcg cgacctacga ggcgttcgcg ctgatcgtcg cctcgatggc
     3001 gacccagcac agcaagtggc tcgaggccac cgcggcgctg ccgtggcgcg cgccggtgcc
     3061 gtcgctcaac tacctgctga cctcgacctg ctggcgcaac gaccacaacg ggttcagcca
     3121 ccagggcccg gggttcatgg acacgatcat ctccaagaag ggcacggtcg cgcgggtcta
     3181 cctgccgccc gacggcaact gcctgctgtc ggtcgccgat cactgcctgc gcagcaagag
     3241 ctacgtcaac ctcgtgatca tcgacaagca accccagctg cagtggctgg cgatgcccga
     3301 cgcccgcgcg cactgcgccc gcggcctgtc gatctgggac tgggccggca ccgaggccgg
     3361 ccacgcgccc gacgtcgtgc tcgcctgcgc gggcgacgtg ccgacgctcg agaccgtcgc
     3421 cgccgcggcc tggctgcgca cccacgcccc cgacctgcgc gtccgcgtgg tcaacgtcgt
     3481 cgacctgatg cggctgttct cgccgcgcga gcacccgcac ggcatgcccg acgagctgtt
     3541 cgtcgacatg ttcacgcgcg acgtcgatgt ctgcttctcg ttccacggct acccgggcgc
     3601 gatccaccag ctgctccacg cccggcccaa cccgccgcgg ttccacgtcc gcggctacaa
     3661 ggaggagggc acgaccacga ccccgttcga catggtcgtg ctcaacgaga ccagccggta
     3721 ccacatcgcg atcgacgcga tccgccgcgc gcgccggccg ccgcccaacg ccgccgagct
     3781 gaccgccgag tgccggcgcc tgctcgacga gcaccgcgcc tacgtcgcga cccacttcga
     3841 ggacctgccc gagatccgcg actggacctg gcggccgtga ttgcaccgga ccccggatcc
     3901 ggtgcaacgg gatcgtttac ctgatgaacc tggccatgac ggtgaccggc gtggcgctgc
     3961 tgttccgcgc cgcccgccgc ggcgccacga tgtcccgtcg cgtgctggtc ggtgcgatgc
     4021 tcggcggctg ggggctgctc accgtcgtcg agggcatgat cgatcaccag ctgctcggtg
     4081 cgcatcacac gcactccgga cccgaccagc tcgcgtggga cgtcgtctac ctgatgttcg
     4141 gcgcagcgct gatgctgatc ggtgccgcga tcgcgtgcag ccgcagcgaa gcgacgtgcc
     4201 ccccgtgcgc ccccacgacg ccgagctgag ccggcaccgc gccctccggg cctcgccgga
     4261 tcagcccgcg gccggccggc ggccccacgc cgagatcatc ggcgacatcg cgaggtcgag
     4321 ctcgccggcg tcgacgctgg cgaagtggcg ctggatctcg tcggcggtca cccgcccgct
     4381 ggcgatcagc cgatcgcgga catgttcgat cgtcctgcgc tccagcgcgg cggacgcggg
     4441 cagcgcgacc gggaagtacg cgtcggccgc gacgtcgcac agcccggcgt cgcggagcgc
     4501 gcgcggcagc tgccg
//
LOCUS       DASVOR010000560         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_502044,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000560 DASVOR010000000
VERSION     DASVOR010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502044"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF516_11115"
     CDS             <1..310
                     /locus_tag="VF516_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108271.1"
                     /translation="AALKPIAAAATATDDDPAAPDAKAAAAASVGPPPPRTEWRTFYV
                     VDPSAGDARVRQEQYFVHTADDLVWASYTDARGELTTEHIPAETARDSISPASRESEY
                     "
     gene            complement(558..1208)
                     /locus_tag="VF516_11120"
     CDS             complement(558..1208)
                     /locus_tag="VF516_11120"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8108272.1"
                     /translation="MPSSSRLVRKPTAAPRRSLRVVGSPEGAREIEREHHAFEAACLP
                     HQAELFGVAMRICRDPDTAKDLVQETLLRAMCAWDSFQAGSNLRAWLFRILTNAFING
                     YRKRRRHQRFATERPGDALAALYGRDQDCTEDLAVELFGDELCDEVKTALGRLGPEYR
                     DVVERADLRGEKYKDIADALDVPIGTVMSRLFRARRVLEGELAGFAARDYGIKRAA"
BASE COUNT          257 a          575 c          550 g          221 t
ORIGIN      
        1 cgccgcgctc aagcccatcg ccgcagccgc caccgccacc gacgacgatc cggcggcgcc
       61 cgatgccaag gcggccgccg cggcctcggt cggcccgccg ccgccgcgca ccgagtggcg
      121 cacgttctac gtcgtcgacc ccagcgcggg cgatgcccgg gtccgccagg agcagtactt
      181 cgtccacacc gccgacgacc tggtgtgggc cagctacacc gacgcgcgcg gcgagctcac
      241 caccgagcac atcccggccg agaccgcgcg cgacagcatc tcgccggcga gccgcgagtc
      301 cgagtactga atgaagagcc gtggatccag ggagcagacg ttgagcttga cctcgacgtc
      361 gacgcaaggc gttgactttg acggcaacgt cgacgtctga ctccaccgtc gacgttgaac
      421 ctggacccag cactagcggc gagaatcctc ttcgaggcgg cgcccagggg ctcaccgtcg
      481 agggtcgacg gtggtgtcta cgtccaacgt cgccgtcgac ctcgacgttc gggtcaaggt
      541 ccaggtcatc gtcaaggtca ggcggcgcgc ttgatgccgt agtcgcgcgc ggcgaacccg
      601 gcgagctcgc cctcgagcac gcggcgggcg cggaacagcc gcgacatcac ggtgccgatc
      661 ggcacgtcga gcgcgtcggc gatgtccttg tacttctcgc cgcgcaggtc ggcgcgctcg
      721 accacgtcgc ggtactcggg gccgagccgg ccgagcgcgg tcttgacctc gtcgcacagc
      781 tcgtcgccga acagctcgac cgcgaggtcc tcggtgcagt cctggtcgcg gccgtacagc
      841 gcggccagcg cgtcgcccgg gcgctcggtc gcgaaccgct ggtggcggcg gcgcttgcgg
      901 tagccgttga tgaacgcgtt ggtgaggata cggaacagcc aggcgcgcag gttcgagccg
      961 gcctggaagc tgtcccacgc gcacatcgcg cggagcaggg tctcctgcac gaggtccttg
     1021 gcggtgtcgg ggtcgcggca gatccgcatc gccacgccga acagctcggc ctggtgcggc
     1081 aggcaagcgg cttcaaacgc atgatgctcg cgttcgatct ctcttgcccc ctccggcgat
     1141 ccgaccaccc ggagcgaccg gcgcggcgca gcggtgggct tgcggacgag gcgggagctg
     1201 gagggcacag acttggtcga gctcatcggg gttcgcgaca tgcccgaggc tcaaaagcag
     1261 ccgacgtgcc acatggaact accgagaaca acagtgtgtt ttttgcctcg aggtcaggga
     1321 gacacgcgat gcgtctccag ccgaacacga gcgcctacat tctgagcctg gctgaccctg
     1381 ggcactgacc gggtgcgatc ccggccacgc cgccacgcat cacgcgatcg ctcgcaggtg
     1441 acatccccgt ggcgcgtgcg agacggatca cacgcgcggc acgcgcgaga cgccgcccag
     1501 cgatcggatc gcctccgcga tcgcctcggg cagatccgag gacaccacgc cgcgatcgcc
     1561 gtggcgccgc gccagcgcat cgccggccgc gccgtgcacg aac
//
LOCUS       DASVOR010000561         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_93289,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000561 DASVOR010000000
VERSION     DASVOR010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93289"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1757
                     /locus_tag="VF516_11125"
     CDS             <1..>1757
                     /locus_tag="VF516_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX8108273.1"
                     /translation="GMGQGLFANSPTFAAVIRRCAAALAPHVDWDLVACITGEAGPAW
                     MTRVDMLQPALWATSVALAELWREAGVEPDVVVGHSQGEIAAATVAGILSCEDAARVV
                     ARRSQIVRRKSGHGLMLAVDLDVAGARAALAGFESRISLAANNGPTSCVLSGDADAVT
                     TLQELLEADDIFCRLVQVDFASHSHHMDELAEDLCAALDGIAPRPAATALMSTVCVEP
                     LAGTEMDASYWVRNLRAPVRFADAMARLFEDGVTHAVEISPHPVLVPAIEQLVAARRA
                     PVSVLATLHRNAGAPRDFALATAHAFVAGLAPFQHLDRAAAVKLPAYPWQRKKYWVAP
                     RKRRGARNPELALALLPAPGEPDAWQGGLELGLDHPPWLADHKVHDAVVVPGAVMLAL
                     ALDAARVRTGSLPRALVDVRFHNPLALGDEPVPASIAWRDDVAGGGSFTLGSLPAGAA
                     AWTPHATARTHGGARVHDAAFPHELQAAPATCGEAFYAGCAARGLCYGPAFRGVVHVF
                     VDGDRALGEVRLPAACRAGARPHGLHPALWDAALQVCLALCPGDHTVVPTAVARVAIL
                     VDLAQPVTALWSHVVRRDA"
BASE COUNT          197 a          656 c          657 g          247 t
ORIGIN      
        1 cggaatgggg cagggcctgt tcgcgaatag cccgacgttc gccgcggtga tccgccgctg
       61 cgccgccgcg ctggcgccgc acgtcgactg ggatctggtc gcatgcatca cgggcgaggc
      121 cgggcccgcg tggatgacgc gggtcgacat gttgcagccg gcgctgtggg ccacgtcggt
      181 cgcgctcgcc gagctgtggc gcgaggccgg ggtcgagccc gacgtcgtgg tcggccacag
      241 ccagggcgag atcgccgcgg cgacggtcgc cggcatcctg agctgcgagg acgccgcacg
      301 cgtggtggcg cggcggagcc agatcgtgcg ccgcaagtcg ggccacggcc tgatgctcgc
      361 cgtcgatctc gacgtggcgg gcgcccgcgc ggcgctcgcc ggcttcgaga gccggatctc
      421 gctggcggcg aacaacggcc cgacctcgtg cgtgctgtcc ggtgatgccg acgcggtgac
      481 cacgctccag gagctgctcg aggcagacga catcttctgc cgcctcgtgc aggtcgactt
      541 tgcgtcgcac agccaccaca tggacgagct cgccgaggac ctgtgcgccg cgctcgacgg
      601 catcgcgcca cgtccggcgg ccaccgcgct gatgtcgacg gtgtgtgtcg agccgctcgc
      661 gggcaccgag atggacgcgt cgtactgggt acgcaacctt cgcgcgccgg tgcggttcgc
      721 cgacgcgatg gcccggctgt tcgaggatgg cgtcacccac gccgtggaga tcagcccgca
      781 cccggtgctc gtgccggcga tcgagcagct ggtggcggca cgccgcgcgc cggtgagcgt
      841 gctggccacg ctgcaccgca acgccggagc cccgcgcgac ttcgcgctcg ccaccgccca
      901 cgcgttcgtg gccgggctcg cgccgttcca gcacctggat cgcgccgcgg cggtgaagct
      961 cccggcgtat ccctggcaac gcaagaagta ctgggtggct ccgcgcaagc gccgcggcgc
     1021 acgcaacccg gagcttgcgc tcgcgctgct ccccgcaccg ggagagccgg acgcgtggca
     1081 aggcgggctc gagctcgggc tcgatcaccc gccgtggctc gcggatcaca aggtccacga
     1141 tgcggtcgtg gtgccgggcg cggtgatgct ggcgctcgcg ctcgacgccg cgcgggtgcg
     1201 caccggatcg ctgccgcgcg ccctggtcga cgttcggttt cacaaccctc tcgcgctcgg
     1261 cgacgagccc gtccccgcga gcatcgcgtg gcgcgatgac gtggccggcg gcggcagctt
     1321 caccctcggg tcgctcccgg ccggcgcggc ggcgtggacc ccacacgcga ccgcgcgcac
     1381 ccacggcggt gcgcgggtcc atgacgctgc gttcccccac gagctccagg ccgcgccggc
     1441 gacctgcggc gaggcgttct acgccggctg cgccgcgcgc ggcctgtgct acgggccggc
     1501 gttccgcggt gtcgtgcacg tgttcgtcga cggcgaccgt gcgctcggcg aggtccgtct
     1561 gcccgcggcc tgccgcgccg gcgcgcgccc gcacggcctc caccccgccc tgtgggacgc
     1621 cgcgctccag gtctgcctcg cgctgtgccc gggcgatcac acggtggtcc cgaccgcggt
     1681 ggcgcgggtc gcgatccttg tggacctcgc gcagccggtc actgcgctgt ggtcgcatgt
     1741 cgtgcggcgc gacgcga
//
LOCUS       DASVOR010000562         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_297507,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000562 DASVOR010000000
VERSION     DASVOR010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297507"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            142..1014
                     /locus_tag="VF516_11130"
     CDS             142..1014
                     /locus_tag="VF516_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108274.1"
                     /translation="MQRRSPLLWLAIVLWNTPYAVRASCGSASPDWHPAMRDINVGIA
                     TREPMQLRGLARSAGLAARLQFASGMQGFRALCVSWIALAGAGSAWADEPSPPPPPPV
                     TSVDHSITIATNLPFLWAQGYSIAGSVSVNLSAHHAIRANVASWQYDNIVAGLHAAEG
                     GGDFVPGDGRTTDIALSWVFYPRRLWDGFLLEVGALHRSRDVTWKDDDDLASFPFTER
                     RTRMDAGRVMIGWSWRINPYLFIATAVGLSDGWEVGSEAERRADGSTARTTPIAQQAG
                     SLEGYLRFGGVIEL"
     gene            1527..2699
                     /locus_tag="VF516_11135"
     CDS             1527..2699
                     /locus_tag="VF516_11135"
                     /inference="COORDINATES: protein motif:HMM:NF040572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX8108275.1"
                     /translation="MADEPKLAAAISLPTNFKIEEIVSRTNVPGLPPVHVTVPPDLGP
                     LAAFVGNWHGNGFNTIFRPNSKATPTPLPRPVGGDNILELNLTSETLSFSSSLGSVPN
                     RGMVQGDIFLNGVPYLQSINDITVPDKPVGIHLEPGLWVIVPATTQPAESVSLVRMAS
                     IPHGTTVLAQGTATVINGAPHIPAVDITPFVTGSPGNKIHFPSQTAATEGTARLPQDL
                     APFIAAGTITQAMLTDPNSVLRDHIAGQTITSTTQISISTSPAAPLFGGGAENIAFLL
                     GQQSPQPHPNARTVNMTATFWIETVEHKIVLPPHLVPAVPLQVKPRLAHPLQPVPTFT
                     ITPPQGITVPAGHTLTVKTKQIQYTQTVLLDFNGLTWPHVSVATLVPAGAIPVGWA"
BASE COUNT          465 a          997 c          988 g          450 t
ORIGIN      
        1 cggcccggag ctcatcgtac cccggtggct cgagcgcgca cgcgacggct tcggcggccg
       61 gatgatcggc cagccggagc gatccgagat cccgctcgac gtcaacgagc ggctgatcgt
      121 cgagttctac gcgcgctgat cttgcagcgg cgatcgcccc tgctgtggct cgcgatcgtg
      181 ctgtggaaca cgccgtacgc cgtccgggcg tcgtgtggct ccgccagccc cgactggcac
      241 cccgcgatgc gcgacatcaa tgtcgggatc gcgacccgcg agcccatgca gttgcgcgga
      301 cttgcgcgat ccgcgggcct ggccgcccgc ctgcaattcg cctctggcat gcagggcttt
      361 cgcgcgctgt gtgtgtcctg gatcgcgctg gcaggggccg gaagcgcgtg ggccgacgag
      421 ccgtccccgc cgccgccgcc gcccgtcacc tcggtcgacc actccatcac gatcgccacc
      481 aacctcccgt tcctgtgggc ccagggctac tcgatcgccg gctcggtgtc ggtgaacctg
      541 tccgcgcacc acgcgatccg cgccaacgtc gcatcgtggc agtacgacaa catcgtcgcg
      601 gggctgcacg ccgccgaggg cggtggggac tttgtccccg gcgacgggcg caccaccgat
      661 atcgcgctca gctgggtgtt ctacccgcgg cgcctgtggg acgggttcct gctcgaggtc
      721 ggcgcgctcc atcgctcgag ggacgtgacc tggaaggatg acgacgacct ggcgagcttc
      781 ccgttcaccg agcggcgaac caggatggat gcagggcgcg tgatgatcgg ctggagctgg
      841 cggatcaacc cgtacctctt catcgccacc gccgtcgggc tctcggacgg ctgggaggtc
      901 ggcagcgagg cggagcgcag ggccgacggc agcaccgcga ggaccacgcc gatcgcgcag
      961 caggccgggt cgctcgaggg ctatctccgg ttcggcggcg tgatcgagct gtgatcgatc
     1021 tccgagcctc ggactgcgat cctcagccgg atgcgcaggg tagccagcac ggggtcgtgc
     1081 ctccgcgcgg cggccggagc tcctatggcc gactccggac tgggcagcgc gtggcaacga
     1141 ctgccatgtg gatcgccatc gggtcgcgcc gcgatggccg cggaatgctg cgcgacacgg
     1201 aggcgtcgcc gcgcacgatt gaaattctcc atgacgagcg gacgcgtctg cgttgcaagg
     1261 cgagcggatc acgacggcag tcaggtttct cgtgagtcac gcagtccggt gttcgagtgc
     1321 gccggacgcg cgcatggcag cgatggcctg caatgccgtt gttgacgtgc acggctcgat
     1381 cgatctatgg tggctcgcca cacactctcg agttcgagtg tgtccttacg atgatggtgt
     1441 gacgcgttgg ggagtgagtg gcacaagagt cattttcagt cgcgccgcac gggggacatg
     1501 tcgagatatg gacggaggag gaactcatgg cggatgaacc gaaactggca gcggcgatct
     1561 cgctgcctac caatttcaag atcgaggaga tcgtctcgcg aaccaacgtc cccgggcttc
     1621 ccccggtgca cgtcacggtg cctccggatc tcgggccgct ggcggcgttc gtcggcaact
     1681 ggcacggcaa cgggttcaac acgatcttcc ggcccaacag caaggcgacg ccgaccccgc
     1741 tgccccgtcc ggtgggcggc gacaacatcc tcgagctgaa cctcacctcc gagacgctgt
     1801 cgttctcgtc gagcctgggc tcggtgccca accggggcat ggtccagggc gacatcttcc
     1861 tcaacggcgt gccgtatctg cagtcgatca acgacatcac ggtcccggac aagccggtcg
     1921 ggatccacct cgagccgggg ctgtgggtga tcgtgccggc gacgacgcag ccggccgaga
     1981 gcgtgagcct ggtccgcatg gcctcgatcc cgcacggcac cacggtgctc gcgcagggca
     2041 cagcaacggt gatcaacggc gcgccgcaca tccccgcggt ggacatcacg ccgttcgtga
     2101 ccggatcgcc gggcaacaag atccacttcc cgagccagac cgcggcgacc gagggcaccg
     2161 cgcggctgcc gcaggatctg gcgccgttca tcgccgcggg cacgatcacg caggcgatgc
     2221 tgaccgatcc gaactcggtg ctgcgcgatc acatcgccgg gcagaccatc acgtcgacga
     2281 cccagatctc gatctcgacc agcccggcgg cgccgctgtt cggcggcggc gcggagaaca
     2341 tcgcgttcct gctcgggcag cagagcccgc agccacatcc gaacgcgcgg accgtgaaca
     2401 tgacggcgac gttctggatc gagaccgtcg aacacaagat cgtgctgccg ccccacctgg
     2461 tcccggccgt cccgctccag gtcaagccgc ggctggccca cccgctgcag ccggtgccaa
     2521 cgttcacgat cacgccgccg caggggatca cggtgccggc ggggcatacg ctcacggtga
     2581 aaaccaagca gatccagtac acccagaccg tgctgctcga tttcaacggc ctgacctggc
     2641 cgcacgtctc ggtggcgacg ctggtgccgg cgggagccat cccggtcggc tgggcgtgag
     2701 ctgagccaag ggatctccga gcctcggact gcgagcctca ggcaaccgga tccggggcgc
     2761 ccgcgttgcg ctgccggtag aacgtgagcg cgttgcgatc caggtcgtag agcgcgaact
     2821 cgcgcgtgcc ccacggcgta tcgcgaatgc caccgggagt gcactcgacg ccgcgctgct
     2881 gatattcggc aaggagcgcg
//
LOCUS       DASVOR010000563         1836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_384963,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000563 DASVOR010000000
VERSION     DASVOR010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384963"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VF516_11140"
     CDS             complement(<1..472)
                     /locus_tag="VF516_11140"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX8108276.1"
                     /translation="MKYVRQFGGGSAEGRASDKALLGGKGANLAEMASLGLPVPPGFT
                     ITTEVCRHVMAHGAGSYPEGLDAEVAQALRRVEELTKNTFGDARQPLLVSVRSGAPAS
                     MPGMMDTILNLGLNDTTVAALAQRSGNPRFAWDCYRRFVAMYGEVVMGVVAPSEQ"
     gene            complement(469..>1836)
                     /gene="glyS"
                     /locus_tag="VF516_11145"
     CDS             complement(469..>1836)
                     /gene="glyS"
                     /locus_tag="VF516_11145"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEX8108277.1"
                     /translation="GEYPVGLSGSFDRAFLEVPEELIVTEMRNHQRYFAMEDARGHLA
                     NRFATLMATVVKDPDVVRRGNEYVIASRLADARFFFAEDQKKSLEQWNERLAPVVFQA
                     RLGDAAKTIGAKLARIEAITGALVERVACNKDVARRAAPLCKADLASAVVGEFPELQG
                     VMGKHYARLAGLPGEVAVAIEEHYFPRGQGGALPSTVEGALVAIADRIDTLVGCFAAG
                     LQPSGSADPFGLRRAALGVLQILLDRGPGGAHFAPDTGWPMRIDVLFEIARNAYGDTL
                     ATAAAREPLREFFRTRLRGLLIEDGLAAQDVDVVLGVSADEPCDARMRARAVAVVPPA
                     AREVFKRIANILDDARAKQHLISGEVKPGAFVSASGAEARLWNAFTERQARLSRALEH
                     HQYRDSFAVLAELGPDVAAFFDRGGVMVMDPDPVLRENRLSLLSRIYEPFARIADFRQ
                     LGGAS"
BASE COUNT          264 a          679 c          646 g          247 t
ORIGIN      
        1 cctgctcgct gggcgcgacc acgcccatca cgacctcgcc gtacatcgcg acgaaccggc
       61 ggtagcagtc ccacgcgaac cgcgggttgc ccgagcgctg ggcgagcgcg gcgaccgtgg
      121 tgtcgttgag cccgaggttc aagatcgtgt ccatcatccc gggcatcgag gccggcgcgc
      181 cggaccgcac gctgaccagc agcggctgcc gggcgtcgcc gaacgtgttc ttcgtcagct
      241 cctcgacccg ccgcagcgcc tgggcgacct cggcgtcgag cccctcgggg tagctgcccg
      301 cgccgtgcgc catcacgtgg cggcacacct cggtcgtgat cgtgaagccg ggcggcaccg
      361 gcaggccgag cgacgccatc tcggcgaggt tggcgccctt gccgccgagc agtgccttgt
      421 cggatgcgcg gccctcggcc gagccgccgc cgaactgtcg gacgtacttc atgacgcacc
      481 tccgagctgg cggaagtctg cgatccgggc gaacggctcg tagatccgcg acagcagtga
      541 caaccggttc tcacgcagca cgggatcggg atccatcacc atgactcctc ctcgatcgaa
      601 gaacgctgcg acgtcggggc cgagctcggc gagcaccgcg aagctgtcgc ggtactggtg
      661 gtgctccagc gcgcgcgaca gccgggcctg gcgctcggtg aacgcgttcc acagccgggc
      721 ctcggcgccg ctcgccgaga cgaacgcacc gggcttgacc tcgcccgaga tcaggtgctg
      781 cttggcccgc gcgtcgtcga ggatgttcgc gatccgcttg aacacctcgc gcgcggccgg
      841 gggcacgacc gcgaccgcgc gcgcccgcat ccgcgcgtcg cacggctcgt ccgccgacac
      901 gccgagcacg acgtcgacgt cctgcgcggc gagcccatcc tcgatcagga ggccgcgcag
      961 ccgggtgcgg aagaactcgc gcagcggctc gcgcgcggcg gcggtcgcca gggtgtcgcc
     1021 gtaggcgttg cgagcgatct cgaacagcac atcgatccgc atcggccagc ccgtgtcggg
     1081 agcgaaatgc gcgccgccgg ggccgcgatc gagcaggatc tggagcacgc cgagcgccgc
     1141 gcggcgcagg ccgaacggat cggccgagcc cgacggctgg aggccggcgg cgaagcagcc
     1201 gaccagggta tcgatccggt cggcgatcgc gaccagcgcg ccctcgaccg tcgacggcag
     1261 cgccccgccc tggccccggg ggaagtagtg ctcctcgatc gcgaccgcga cctcgccggg
     1321 caagccggcg agccgcgcgt agtgcttgcc catcacgccc tggagctcgg ggaactcgcc
     1381 gaccaccgcg ctcgcgagat cggccttgca caggggcgcg gcgcgccgcg cgacgtcctt
     1441 gttgcacgcg acccgctcga ccagcgcgcc ggtgatcgcc tcgatccggg ccagcttggc
     1501 gccgatcgtc ttggccgcgt cgccgagcct ggcctggaac accaccggcg cgagccgctc
     1561 gttccactgc tcgagcgact tcttctggtc ctccgcgaag aagaaccggg cgtcggcgag
     1621 ccgcgacgcg atcacgtact cgttgccgcg gcggacgacg tccggatcct tgaccacggt
     1681 cgccatcagc gtcgcgaacc ggttcgcgag atggccccgg gcgtcctcca tcgcgaagta
     1741 gcgctggtgg ttgcgcatct cggtgacgat cagctcctcg gggacctcga ggaacgcgcg
     1801 gtcgaagctg ccgctgaggc cgaccgggta ctcgcc
//
LOCUS       DASVOR010000564         6345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_63610,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000564 DASVOR010000000
VERSION     DASVOR010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6345
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63610"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF516_11150"
     CDS             complement(<1..272)
                     /locus_tag="VF516_11150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108278.1"
                     /translation="MRPGVVLVLALAAVGGVPVVGGVPGCGGRTTTAPPEKKDGGPSG
                     APGPSDASVEVDAVALGLPDLGAYGWRKRGGQPAFRVAVQAEARGD"
     gene            394..1002
                     /locus_tag="VF516_11155"
     CDS             394..1002
                     /locus_tag="VF516_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108279.1"
                     /translation="MSTRRVLTLSAATAATIAASVSASALGGCQGKSEPDPPRPPAPV
                     IVARAGSTTLAHIIAGHPCRAEIDGDELLIGTQPLVAQVGNTRWSGDDTAGDGLTTLR
                     KDGAAVVRIRDAQEAAIEVFDPAGTAILRLSADGAIANGSGEILRRAEAARGSIKVGE
                     AVVTGTTDVALGVLITAPELIPEVRGLAACHRLFWHEQAGRK"
     gene            1008..2840
                     /gene="typA"
                     /locus_tag="VF516_11160"
     CDS             1008..2840
                     /gene="typA"
                     /locus_tag="VF516_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEX8108280.1"
                     /translation="MPAPSIRNVAIIAHVDHGKTTLVDGLLKQAGTFRAGEVVAERAM
                     DSNDLERERGITILSKCTAVTWKGTRINIVDTPGHADFGGEVERVLGMVDSVLLLVDA
                     YEGPMPQTRFVTQKAFEMGLSPIVVVNKIDRPGVDPHAVMDQVFELFISLGASDAQLD
                     FPVIYASGREGYAIRELGEPPVDLGPMLDLIVEKVPPAQGDPDAPLVLQVATLDYDDY
                     LGYMAIGRMRGGRTKVGDRVLLAHRDGSREEFRVQKVLGFQGLKRFELAEALAGDIVA
                     ITGMENLNVGETITSIAEPTILPLLKIDAPTITMNFRVNDGPFAGKEGKYVTSRNLRE
                     RLEREIKSNVALRVEETADAGVFKVSGRGELHLSVLIETMRREGYELCVSQPQVILQY
                     DAAGKITEPYEDAVIDLDEAYVGAVVSELNRRAGVMREMRPSAPGRVRLEYRIPARGL
                     IGYRSQFLTDTRGTGVLYTQFAEYGPQAPDIRTRQNGVLIASEEGVSNAYGLFYLQER
                     GQMFIGPNVRVYAGMVVGIHARDNDLVVNPSKTKKLTNIRTTAADEKLVLAPPRQITL
                     EYALEFINDDELVEVTPQSIRLRKAILDHNERKRYEKKPADVDA"
     gene            complement(2853..3851)
                     /locus_tag="VF516_11165"
     CDS             complement(2853..3851)
                     /locus_tag="VF516_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108281.1"
                     /translation="MSIPADFDPKPTLPTGGLDRMIRIWEALRDANTGGYGDSVLRQI
                     SGWGKPSPKNMTSCSPFTATCIGMMFDPGDAIDGATVYAPVINVAGGMLPLPQDFYVL
                     HNGFYFEVDPDVPGSADRIAARKKQFSDHGWRRCDDSVLSTAFFNLGYRVDIRDVRRG
                     DLIGIDWGTGGGHAGFVWDVHTNRDGAVDCLQFVAANGFGHAGAWHGPGVSVSAPNDA
                     AAFIDHNADGTYTKKFPLFTDNELYLTGASWYCLPGVTRQEVDRSTFRSPAPPIEHFL
                     DKATPGKHYIEELRALRFWGFAPPETPHGTTLTDDKRELAQALCQWPTTPPWCMAG"
     gene            complement(4088..4333)
                     /locus_tag="VF516_11170"
     CDS             complement(4088..4333)
                     /locus_tag="VF516_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108282.1"
                     /translation="MRITVPSGGCAAGGSSSQVFFSLPYRIDSSTCSADGPAEGPADG
                     SLADPPPGPRVLLAPRRQRRRHHPCDDETLGMHSRRT"
     gene            complement(4383..5627)
                     /locus_tag="VF516_11175"
     CDS             complement(4383..5627)
                     /locus_tag="VF516_11175"
                     /inference="COORDINATES: protein motif:HMM:NF020129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEX8108283.1"
                     /translation="MKHRIGIHALGLAIPRRYIAMEDLARARGVDPAKYIQGLGAREM
                     AVAEPGEDTVALAALAARRALVAGDVSPGRLGMLVVGTETGVDHSKPVASFVHGLLAL
                     PSAMRVYDTQHACYGGTAGVMAAVEWIASGAADGRAALVVCSDIARYGLSTAGEPTQG
                     AGAVAMVIAAEPGLIELDVGISGAASSHVHDFWRPLGLREAQVDGHYSVQCYLDAVAT
                     AYRGWRGRAVARERVRGAGLASEQLARICYHVPFCKMAKKAHLHVRRCDVEDAGGAWD
                     PAAEDRAAAAFRDQVEPSLGLCARIGNIYTGSLYLGLAGLLHAQADPLAGARIGLFSY
                     GSGCTSEFFSGVVAPGAAARIAASQIDELLAARERISVTEYERIMALPPTAPLVPWPG
                     HASGGQVRFVGVSNHQRRYASS"
     gene            complement(5662..>6345)
                     /locus_tag="VF516_11180"
     CDS             complement(5662..>6345)
                     /locus_tag="VF516_11180"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8108284.1"
                     /translation="GGYAGLMVAPVGGIGHVPAGIPWTVAATVCCTAGTAVHVVRTRG
                     RVQRGETVVITGASGGVGLQAVQLARLDGARVIAVTSSTRKSRALYNAGAHEVVIAPD
                     LEFASEVRRLTGGDGAQVAIEIVGSATFAQTLKAMAPGGRVVVVGNLESGTIALNPGL
                     VIVKELEILGAYATTAGELETALRLTAGGKLASYVTDVLPLGDAAKAHTRLENREVAG
                     RLVLCPDPV"
BASE COUNT          931 a         2282 c         2282 g          850 t
ORIGIN      
        1 cagtcgccgc gcgcctcggc ctggacggcg acgcggaacg cgggctggcc gccgcgcttg
       61 cgccagccgt acgccccgag atcgggcagg ccgagcgcga ccgcgtcgac ctcgacggag
      121 gcgtcgctgg ggcccggcgc ccccgacggc ccgccgtcct tcttctcggg tggcgcggtg
      181 gtcgtgcgac caccacatcc cggcaccccg ccgacgaccg gcaccccgcc gacggcggcg
      241 agcgcgagca ccagcacgac cccggggcgc atccttccag cgacgtaaca cggctggccg
      301 cgacgcgcga ctcgcgtggt tgcggccgct gaaaacccgg gcgcggctgc gggttgccga
      361 ccccgccgca tcctgttatg aacccgccgc cccgtgtcga cccgacgtgt gctcacgctg
      421 tccgccgcca ccgctgccac gatcgcggcc tcggtatcgg cctcggcgct cggcggctgc
      481 cagggcaagt ccgagccgga tccgccgcgg ccgccggctc cggtgatcgt ggcgcgcgcg
      541 ggcagcacca cgctcgccca catcatcgcc ggtcacccgt gccgcgccga gatcgacggc
      601 gacgagctcc tgatcggcac ccagccgctg gtcgcgcagg tcggcaacac ccggtggtcg
      661 ggcgacgaca ccgcgggcga cggcctcacg accttgcgca aggacggcgc cgcggtggtc
      721 cggatccgcg acgcgcagga agcggcgatc gaggtgttcg accccgcggg cacggcgatc
      781 ctgcggctgt cggccgacgg cgcgatcgcc aacggcagcg gcgagatcct gcgccgtgcc
      841 gaggccgcgc gcgggtcgat caaggtcggc gaggcggtcg tcaccgggac gaccgacgtc
      901 gcgctcggcg tgctgatcac cgcgcccgag ctgatccccg aggtccgcgg gctcgccgcg
      961 tgccatcgcc tgttctggca cgaacaggca ggcaggaagt agtcgccatg cccgctccca
     1021 gcatccgcaa cgtggcgatc atcgcccacg tcgatcacgg caagaccacg ctggtcgatg
     1081 gcctgctcaa gcaggcgggc acgttccgcg ccggcgaggt ggtcgccgag cgcgcgatgg
     1141 actccaacga cctcgagcgc gagcgcggca tcacgatcct gtcgaagtgc accgcggtga
     1201 cctggaaggg cacgcggatc aacatcgtcg acaccccggg ccacgccgac ttcggcggcg
     1261 aggtcgagcg cgtgctcggc atggtcgaca gcgtgctgct gctggtcgac gcctacgagg
     1321 ggccgatgcc gcagacccgg ttcgtgaccc agaaggcgtt cgagatgggg ctgtcgccga
     1381 tcgtcgtggt caacaagatc gaccggcccg gcgtcgatcc gcacgcggtg atggatcagg
     1441 tgttcgagct gttcatctcg ctcggcgcga gcgacgccca gctcgacttc ccggtgatct
     1501 acgcgtcggg ccgcgagggc tacgcgatcc gcgagctcgg cgagccgccg gtcgacctcg
     1561 ggccgatgct cgatctgatc gtcgagaagg tgccgccggc gcagggcgac cccgacgcgc
     1621 cgctcgtgct gcaggtcgcg acgctcgact acgacgacta cctcggctac atggcgatcg
     1681 gccggatgcg cggcgggcgg accaaggtcg gcgaccgcgt gctgctcgcc caccgcgacg
     1741 gcagccggga ggagttccgg gtccagaagg tgctcggctt ccagggcctc aagcggttcg
     1801 agctcgccga ggcgctcgcc ggcgacatcg tcgcgatcac cgggatggag aacctcaacg
     1861 tcggcgagac catcacctcg atcgccgagc cgacgatcct gccgctgctc aagatcgacg
     1921 cgccgacgat cacgatgaac ttccgggtca acgacgggcc gttcgcgggc aaggagggca
     1981 agtacgtcac gtcgcgcaac ctgcgcgagc ggctcgagcg cgagatcaag agcaacgtcg
     2041 cgctgcgcgt cgaggagacc gccgacgccg gcgtgttcaa ggtgtccggc cgcggcgagc
     2101 tccacctctc ggtgctgatc gagaccatgc ggcgcgaggg ctacgagctg tgcgtgtcgc
     2161 agccccaggt gatcctccag tacgacgccg ccggcaagat caccgagccc tacgaggacg
     2221 cggtgatcga tctcgacgag gcctacgtcg gcgcggtggt gtccgagctc aaccgccggg
     2281 ccggcgtgat gcgcgagatg cggccgtcgg cgcccggccg ggtccgcctg gagtaccgga
     2341 tcccggcgcg cggcctgatc ggctaccgct cgcagttcct gaccgacacc cgcggcaccg
     2401 gcgttttgta cacccagttc gccgagtacg gcccgcaggc ccccgacatc cggacgcgcc
     2461 agaacggcgt gctgatcgcc agcgaggagg gcgtgtccaa cgcctacggc ctgttctacc
     2521 tccaggagcg cggccagatg ttcatcgggc ccaacgtccg ggtctacgcc gggatggtcg
     2581 tcggcatcca tgcgcgggac aatgacctgg tggtcaaccc gtccaagacc aagaagctga
     2641 ccaacatccg gaccaccgcg gccgacgaga agctggtcct cgcgccgccg cgccagatca
     2701 cgctcgagta cgcgctggag ttcatcaacg acgacgagct ggtcgaggtc acgccgcagt
     2761 cgatccggct gcgcaaggcg atcctcgacc acaacgagcg caagcggtac gagaagaagc
     2821 cggccgacgt cgacgcctga cgccgggcag gcctaccccg ccatgcacca cggcggcgtc
     2881 gtgggccact ggcacagcgc ctgtgccagc tcgcgcttgt cgtcggtcag cgtcgtgccg
     2941 tgcggcgtct cgggaggtgc gaagccccag aaccggagcg cgcgcagctc ctcgatgtag
     3001 tgcttgccgg gggtcgcctt gtccaggaag tgctcgatgg gcggcgccgg cgagcggaac
     3061 gtgctgcggt cgacctcctg ccgcgtgacg ccggggaggc agtaccacga ggcgccggtg
     3121 aggtagagct cgttgtcggt gaacagcggg aacttcttgg tgtacgtgcc atccgcgttg
     3181 tggtcgatga acgccgcggc gtcgttcggc gcggacaccg acacgcccgg gccgtgccag
     3241 gcccccgcgt ggccgaaccc gttggccgcg acgaactgca ggcagtcgac cgcgccgtcc
     3301 cggttggtgt gcacgtccca cacgaaccct gcatggccgc cgccggtccc ccagtcgatc
     3361 ccgatcaggt cgccgcggcg gacgtcgcgg atatcgaccc ggtagccgag gttgaagaac
     3421 gcggtcgaca gcacggagtc gtcgcaccgc cgccagccgt ggtcggagaa ctgcttcttg
     3481 cgcgcggcaa tcctgtccgc cgagcccggc acgtccgggt cgacctcgaa gtagaagccg
     3541 ttgtgcagca cgtagaaatc ttgcggcagc gggagcatcc ctccggcgac gttgatcacg
     3601 ggcgcgtaca cggtcgcgcc atcgatcgcg tcgccggggt cgaacatcat gccgatgcag
     3661 gtcgccgtga acggcgagca cgacgtcatg ttcttgggac tgggcttgcc ccaccccgag
     3721 atctggcgca gcacgctgtc tccgtacccg ccggtgttcg catcgcgcag cgcctcccag
     3781 atccggatca tccgatcgag tccgccggtc gggagcgtgg gcttcggatc aaaatcagct
     3841 ggaatcgaca tgtcgagacc ctccttcaga catcaccctt gatcgatctt ctcggatgat
     3901 acatccgatc ggcgtttcct cattgcactg gatgtgccgg gacccatcgg tcgcccaggc
     3961 cccggatggc tcgtgggacg cggtgagcga ctcccgcgaa accatgaagt tcttgcgtca
     4021 cagcggaccg gagctgtgat ctcacgcggg cgccatggcg aggttcccgg cctgccacac
     4081 ggcgtcgtca cgttcgacgc gaatgcatac caagcgtctc gtcatcgcag gggtggtggc
     4141 ggcggcgctg gcggcggggt gcaagcagga cgcgcggccc cgggggcgga tcggcgagcg
     4201 acccgtcggc cgggccctcg gcgggcccgt cggccgaaca ggtcgagctg tcgatcctgt
     4261 acggcagcga gaagaagacc tggctcgagc tgccaccggc cgcgcagccg cccgacggca
     4321 cggtgatccg caacctgctg atgatgtggt cgcgcgtcgt ccagccgaag tgagcgcgcg
     4381 cctcagctgc tcgcgtaccg gcgctggtgg ttcgacacgc cgacgaaccg gacctggccg
     4441 ccggaggcat ggcccggcca cggcacgagc ggggcggtcg gcggcagcgc catgatccgc
     4501 tcgtactcgg tgaccgagat ccgctcgcgc gccgcgagca gctcgtcgat ctgcgaggcg
     4561 gcgatccgcg ccgcggcccc gggagcgacc acgccggaga agaactcgct ggtgcagccg
     4621 ctgccgtagg agaacagccc gatccgcgcg ccggcgagcg ggtcggcctg ggcgtggagc
     4681 agcccggcga gcccgaggta gagcgagccg gtgtagatgt tgccgatccg ggcgcacagc
     4741 ccgagcgacg gctcgacctg atcgcggaac gcggcggccg cgcggtcctc ggcggcgggg
     4801 tcccacgccc cgcccgcgtc ctcgacgtcg cagcgccgga cgtggaggtg cgccttcttc
     4861 gccatcttgc agaacggcac gtggtagcag atccgcgcca gctgctcgct ggccaggccg
     4921 gcgccgcgga cccgctcgcg cgccaccgcg cggccgcgcc agccgcgata ggcggtcgcg
     4981 accgcgtcga ggtagcactg cacgctgtag tggccgtcga cctgggcctc gcgcaggccg
     5041 agcggccgcc agaagtcgtg gacgtggctc gacgcggcgc ccgagatccc gacgtcgagc
     5101 tcgatcagcc cgggctcggc cgcgatcacc atcgcgaccg cgccggcgcc ctgggtcggc
     5161 tcgcccgcgg tcgacaggcc gtagcgcgcg atgtccgagc acacgacgag cgccgcgcgg
     5221 ccgtcggccg cccccgaggc gatccactcg accgcggcca tcaccccggc ggtgccgccg
     5281 tagcacgcgt gctgggtgtc gtagacccgc atcgcggacg gcagcgcgag caggccgtgg
     5341 acgaacgacg cgaccggctt gctgtgatcg acgccggtct cggtgccgac gacgagcatg
     5401 ccgaggcggc cgggggagac gtcgccggcg accagcgcgc ggcgggcggc gagcgcggcg
     5461 agcgcgacgg tgtcctcgcc gggctcggcg accgccatct cgcgcgcgcc gaggccctgg
     5521 atgtacttgg ccggatcgac gccgcgggcc cgcgccaggt cctccatggc gatgtagcgg
     5581 cgcgggatgg cgaggccgag ggcgtggatg ccgatgcgat gcttcatggg attcctttcg
     5641 cggccgccgg gcagcggccg gtcacacggg gtcggggcac agcacgagcc ggccggcgac
     5701 ctcgcggttc tcgagccggg tgtgggcctt ggcggcgtcg ccgagcggca gcacgtcggt
     5761 gacgtacgac gccagcttgc cgccggcggt gagccggagc gcggtctcga gctcgccggc
     5821 cgtggtggcg tacgcgccga ggatctccag ctccttgacg atcaccagcc cggggttgag
     5881 cgcgatcgtg ccgctctcga ggttgccgac gacgaccacc cggccgcccg gcgccatcgc
     5941 cttgagggtc tgggcgaacg tcgccgagcc gacgatctcg atcgcgacct gggcgccgtc
     6001 gccgccggtc agccggcgga cctcggacgc gaactcgagg tcgggcgcga tcacgacctc
     6061 gtgggcaccg gcgttgtaca gcgcgcgcga cttgcgggtg ctcgacgtca ccgcgatcac
     6121 ccgcgcgccg tcgagccgcg cgagctggac cgcctgcagg ccgaccccgc cgctcgcgcc
     6181 ggtgatcacc acggtctcgc cgcgctggac ccggccgcgg gtgcgcacga cgtggaccgc
     6241 ggtgcctgcg gtgcagcaca ccgtcgcggc caccgtccac gggatgccgg cgggcacgtg
     6301 gccgatgccg ccgaccggcg cgaccatcag cccggcgtag ccacc
//
LOCUS       DASVOR010000565         4432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_93300,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000565 DASVOR010000000
VERSION     DASVOR010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4432
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93300"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /locus_tag="VF516_11185"
     CDS             <1..1141
                     /locus_tag="VF516_11185"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX8108285.1"
                     /translation="QAPGAFSKYRLGNETETYGELGLTANWVNPEHNDAWFKTKVKLA
                     LVAPRNSTFDSLRDPPGNIAIREAYGEAGHLFESHPEMTFWAGTRFYRRRDVHIIDFF
                     FNDMSGYGGGFQDMKVGGNAKLSVAYLGGSVENQRNAMGNEISADLGRLAKNTLDLRL
                     SDIPAGPGTLEFWLIPTLALQGNSTQNNHSGLGGGVFYFMPVMGGFNEISASFGYNAA
                     ANLSSGLDTGIASGGWLFRLIERGVFQLTPTASMMVSGVLQFDNRDGNPANTTDSSLG
                     NTWVSFGARPVFMLSKYTGVAVEGGVDIVKNQASGSGTEVLGKVTVAGLIRPAADFWA
                     RPELRIFLTTAAWNGAVNNAGGTGGAAFAGDNAGLTAGVQVESWW"
     gene            complement(1175..2065)
                     /gene="malG"
                     /locus_tag="VF516_11190"
     CDS             complement(1175..2065)
                     /gene="malG"
                     /locus_tag="VF516_11190"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HEX8108286.1"
                     /translation="MPIVVSKSHRWRVFAAHAFLIVFLLFTLFPLAMVISISLRPGNF
                     ASGSLIPEHISFEHWRLALGLSYVDVDGRVVTPPFPVLRWLWNSIKVAGASAALMVVL
                     STTSAYAFSRLKFRGKALTLNAIMLLQMFPAVLALVAIYAIFEVIGEHVSWLKVDSLP
                     SVVVAYLGYIALHIWTIKGYFDTIPSEIEEAAQVDGATPFQAFRRVLLPMAVPILAVV
                     FVLAFIYCIIEYPIASVLLQSEDRLTLAVGSRLYLYEQRYLWGDFAAAAVLSGLPITA
                     VFLLAQRWMVSGLTAGGLKG"
     gene            complement(2085..3644)
                     /gene="malF"
                     /locus_tag="VF516_11195"
     CDS             complement(2085..3644)
                     /gene="malF"
                     /locus_tag="VF516_11195"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008232.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HEX8108287.1"
                     /translation="MQPRHRRIASRLAATIAALAGLGAVYQLYVAGNPVLAAACAGAF
                     GLTYFVYTARRAYTYRYLFPGLAGIALFIVLPLIYTVWIGFTNYSSKNLLTFERATEV
                     LLDEVYERSSVRYQFTLHAAGRGFRIVLHTGDEDAPATPAGGPPTAFVSDALPLLDTA
                     PQRITVQPLAGSGFVPGDALPLKDVIAHRDAIRALDVRFPDGTAARMASLREFTPFQK
                     LYQRNPDGSLTNQQTGERITPNVHTGFYETAAGQRIEPGFRVTVGLDNYTRVFTDGKF
                     RGPFIRVFLWTVVFAGVTVVLSAGLGMFLAELLSWEGLRFAWLYRVLLFLPYAVPSFI
                     SILVFKGLFNRNFGEINLILDTLLGIRPNWTGNAWLARGVILIVNSWLGYPYFMLICM
                     GLQKSIPHELYEASALAGAGPLTNFFQITWPLIRKPLSPLLVSAFAFNFNNFVLIYLL
                     TQGRPDFLDTIIPAGETDILVSYTYRIAFEDSGQQFGLASAISTVIFAVVAILSVINL
                     RLTKVTVEDKR"
     gene            3770..>4432
                     /locus_tag="VF516_11200"
     CDS             3770..>4432
                     /locus_tag="VF516_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8108288.1"
                     /translation="MATVVLRDVRKSFDTVEVIRGVSLTIADGEFVVFVGPSGCGKST
                     LLRLIAGLETITAGEVAIDGQRCNDLPPSERGIAMVFQSYALYPHMSVYDNMAFALKL
                     AHMRKDEIRGRVERAAGILQIGGLLDRKPKALSGGQRQRVAIGRAIVREPKVFLFDEP
                     LSNLDAALRVQMRIEIARLHRDLKTTIIYVTHDQVEAMTMASRIVVLNAGLVEQVGTP
                     LEL"
BASE COUNT          779 a         1481 c         1550 g          622 t
ORIGIN      
        1 ccaggcgccc ggcgcgttca gcaagtaccg cctcggcaac gagaccgaga cctacggcga
       61 gctcgggctg accgccaact gggtcaaccc cgagcacaac gacgcctggt tcaagaccaa
      121 ggtcaagctc gcgctggtcg cgccgcgcaa ctccacgttc gactcgctcc gcgatccgcc
      181 gggcaacatc gcgatccgcg aggcctacgg cgaggccggc cacctgttcg agtcgcaccc
      241 cgagatgacg ttctgggccg gtacgcggtt ctatcgccgg cgcgacgtcc acatcatcga
      301 cttcttcttc aacgacatga gcggttacgg cggcggcttc caggacatga aggtcggggg
      361 caacgccaag ctgtcggtcg cttacctcgg cggctcggtc gagaaccagc gcaacgcgat
      421 gggcaacgag atcagcgccg acctcggccg cctggccaag aacaccctgg acctccggct
      481 gtcggacatc cccgccgggc ccggtacgct ggagttctgg ctgatcccca cgctggcgct
      541 ccagggcaac agcacgcaga acaaccacag cggcctcggc ggcggcgtgt tctacttcat
      601 gccggtgatg ggcggcttca acgagatctc ggcgtcgttc ggctacaacg ccgccgccaa
      661 cctgtcgagc ggcctcgaca ccggcatcgc cagcggcggc tggctgttcc ggctgatcga
      721 gcgcggcgtg ttccagctca ctccgacggc gtccatgatg gtgtccggcg tcctgcagtt
      781 cgacaaccgc gacggcaacc cggcgaacac gaccgacagc tcgctcggca acacctgggt
      841 ctcgttcggc gcccgcccgg tgttcatgct cagcaagtac accggcgtcg cggtcgaggg
      901 cggcgtcgac atcgtcaaga accaggccag cggatcgggc accgaggtgc tcggcaaggt
      961 cacggtcgcc ggcctgatcc gtccggcggc ggacttctgg gcccgtcccg agctgcggat
     1021 cttcctcacc acggccgcgt ggaacggtgc ggtcaacaac gccggcggga ccggcggcgc
     1081 ggcgttcgcc ggcgacaacg ccggcctgac ggcaggcgtc caggtcgaga gctggtggtg
     1141 agagcggcgg ggctggcccg cccggccgcc ccggtcagcc cttgaggccg cccgcggtca
     1201 gccccgacac catccagcgc tgcgcgagca ggaacaccgc ggtgatcggc aggccgctca
     1261 gcacggcggc cgccgcgaag tcgccccaca ggtagcgctg ctcgtagagg tacagccgcg
     1321 agccgaccgc gagggtcagc cggtcctcgc tctgcagcag caccgaggcg atcgggtact
     1381 cgatgatgca gtagatgaac gccaggacga acacgaccgc gaggatcggc acggccatcg
     1441 gcaacagcac gcggcggaac gcctggaacg gggtcgcgcc gtcgacctgg gcggcttcct
     1501 cgatctccga ggggatcgtg tcgaagtagc ccttgatcgt ccagatgtgg agcgcgatgt
     1561 agccgaggta ggcgaccacg accgacggca gcgagtcgac cttgagccac gacacgtgct
     1621 cgccgatcac ctcgaagatc gcgtagatcg cgaccagcgc gagcaccgcc gggaacatct
     1681 gcagcagcat gatcgcgttg agcgtcaggg ccttgccccg gaacttcagc cgcgagaacg
     1741 cgtaagccga ggtcgtcgac agcacgacca tgagcgccgc ggaggccccg gcgaccttga
     1801 tcgagttcca cagccagcgc agcaccggga acggcggcgt gaccacccgg ccgtcgacgt
     1861 cgacgtagga cagcccgagc gcgagccgcc agtgctcgaa gctgatgtgc tccgggatca
     1921 gcgagcccga cgcgaagttg ccggggcgca gcgagatcga gatcaccatc gcgagcggga
     1981 acagcgtgaa caggaggaac acgatgagga acgcgtgggc ggcgaacacg cgccatcggt
     2041 gggacttgct gacgacgatg ggcatgacga gctcctgcgc cggatcagcg cttgtcctcg
     2101 acggtgacct tggtgagccg gaggttgatc accgacagga tcgccacgac ggcgaagatc
     2161 acggtggaga tcgccgaggc caggccgaac tgctgcccgg agtcctcgaa cgcgatccgg
     2221 taggtgtagc tgaccaggat gtcggtctcg ccggcgggga tgatggtgtc gagaaagtcc
     2281 ggccggccct gggtcaatag gtagatcaat acgaagttat tgaagttgaa tgcgaacgcc
     2341 gacacgagca gcgggctgag cggcttgcgg atcagcggcc aggtgatctg gaagaagttg
     2401 gtcagcgggc cggcgccggc cagcgcggac gcctcgtaga gctcgtgcgg gatcgacttc
     2461 tggagcccca tgcagatcag catgaagtac gggtatccga gccagctgtt gacgatcagg
     2521 atcacccccc gggcgagcca cgcgttgccg gtccagttgg gccggatccc gagcagcgtg
     2581 tcgaggatca ggttgatctc gccgaagttg cggttgaaca ggcccttgaa caccaggatc
     2641 gagatgaacg acggaaccgc gtagggcagg aacagcagca cgcggtacag ccaggcgaac
     2701 cgcaggccct cccagctcag gagctcggcg aggaacatgc caagcccggc ggatagcacc
     2761 acggtcactc ccgcgaacac cacggtccac aggaacaccc ggatgaacgg gccgcggaac
     2821 ttgccgtcgg tgaacacccg cgtgtagttg tcgagcccga cggtgacccg gaaccccggt
     2881 tcgatccgct ggccggcggc ggtctcgtag aacccggtgt ggacgttcgg ggtgatgcgc
     2941 tctccggtct gctggttggt cagcgagccg tcggggttgc gctggtacag cttctggaac
     3001 ggcgtgaact cgcgcaggct cgccatccgg gcggcggtgc cgtccgggaa ccgcacgtcg
     3061 agcgcccgga tcgcgtcgcg gtgcgcgatc acgtccttga gcggcagggc gtcgcccggg
     3121 acgaacccgg atccggcgag cggctggacc gtgatgcgct gcggcgcggt gtcgagcagc
     3181 ggcagcgcgt cggacacgaa cgcggtcggg ggccccccgg ccggtgtcgc cggagcgtcc
     3241 tcgtccccgg tgtgcaggac gatgcgaaac ccgcgcccgg cggcgtgcag cgtgaactgg
     3301 tagcgcaccg agctgcgctc gtagacctcg tcgagcagga cctcggtggc gcgctcgaac
     3361 gtcagcaggt tcttcgagct gtagttggtg aagccgatcc acaccgtgta gatcagcggc
     3421 agcacgatga acagcgcgat gccggcgagc ccggggaaca gatagcgata ggtgtacgcg
     3481 cgccgcgcgg tgtacacgaa gtacgtcagg ccgaacgcgc cggcgcacgc ggcggccagc
     3541 accgggttgc cggcgacgta gagctggtac acggccccga gcccggcgag cgcggcgatc
     3601 gtggccgcga ggcgcgaggc gatgcgccgg tggcgtggct gcatgcgccg gcagtatatc
     3661 cggcgccgcg atcgacaccg cgcgtcgaac cgcggattcg tcatcgcccg ccgaacgccg
     3721 ccgtccccgg cttgaactgc ggcgaccggc tatggtcgga tcgccgcaca tggccaccgt
     3781 cgtcctgcgc gacgtccgca aatcgttcga caccgtcgag gtcatccgcg gcgtcagcct
     3841 gaccatcgcc gacggcgagt tcgtcgtgtt cgtcggcccg tcgggctgcg gcaaatcgac
     3901 cctgctgcgc ctgatcgccg ggctggagac catcaccgcc ggcgaggtcg cgatcgacgg
     3961 ccagcgctgc aacgacctgc cgccgtcgga gcgcggcatc gcgatggtgt tccagagcta
     4021 cgcgctgtac ccgcacatgt cggtctacga caacatggcg ttcgcgctca agctggccca
     4081 catgcggaag gacgagatcc gcggccgggt cgagcgcgcc gccggcatcc tccagatcgg
     4141 cgggctgctc gatcgcaagc ccaaggcgct gtcgggcggg cagcgccagc gcgtcgcgat
     4201 cggccgggcg atcgtgcgcg agcccaaggt gttcctgttc gacgagccgc tgtccaacct
     4261 cgacgccgcg ctccgggtcc agatgcggat cgagatcgcc cggctgcaca gggacctcaa
     4321 gacgacgatc atctacgtca cgcacgatca ggtcgaggcg atgacgatgg cgagccggat
     4381 cgtcgtgctc aacgccgggc tggtcgagca ggtcggcacg ccgctcgagc tg
//
LOCUS       DASVOR010000566         3833 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_560695,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000566 DASVOR010000000
VERSION     DASVOR010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3833
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560695"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2438)
                     /locus_tag="VF516_11205"
     CDS             complement(<1..2438)
                     /locus_tag="VF516_11205"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8108289.1"
                     /translation="MNGPASPVQRGRESPLSIDEPGTERELAEAQREIRPTGASFLDR
                     SDLDILEAAIAHSRTRLRAQPRDNPSLRIATEWLLDNDYLVRGVFAQLRHELPASFQR
                     RLPRLAGDGRVRALVVAGVILDAMPELDLDELDRFLDDYQERAPLTIAELWALPALLR
                     LAILRRLDELLAQAASEPHALAPSQDVGGAAGRAIRGLRLLAEVDWREVFSRHSAVEQ
                     LLQGDPARAYATMEFETRDQVRRVIEDIATASGRDEIEVARAVVARASAAQPDGRCDH
                     VGYWLVDAGRRPFERSLGAGRRGLPRRLLDHPRLTFFGILAAAQLVLLAPVVLYLAWL
                     GAPLASAVAAVVLCWVPSSVPAATLAHGVLSSLIPPRVLPKLDFSRGIPARWRTLVAV
                     PTMLTDDAEIDRLVMQLEVYYLANPDAELRFALLTDYLDSDEPPGDRERAQLARAAAG
                     IDRLNRRHGTGARHPFYLLHRAPRWNPAEGKWMGWERKRGKLDELNRYLRGDTATSYV
                     LAAGEPGGLDAIAFVVTLDSDTELPPGTAARLAGTLAHPLNRPRPDASGRIRAGYTVI
                     QPRVEISPRSARRSAFSRLFAGDTGFDIYSRAVSEVYQDVFGQGIYVGKGIYDVDSFR
                     ASLAGRVPENALVSHDLFEGIQGRVALASDVVVYEDYPPNYLAYLRRMHRWIRGDWQL
                     VPWLLPHVPVLRGEPRRNDLGALGRFQILDNLRRSLVAPAVLALLVWGWLVLPGHPPV
                     WTLIALLAPGAAAVSSLVRSTSLPRRHLGRWMLAICFAPQETRTVIDAVVRAAVQMLW
                     TRKRMLEWTPAAADA"
     gene            complement(2488..3546)
                     /locus_tag="VF516_11210"
     CDS             complement(2488..3546)
                     /locus_tag="VF516_11210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8108290.1"
                     /translation="MCIAPTACMAGQHSSLGDEPAVAPRPTGFRSVLVCLDRSRAAEA
                     ALPLATHLAKADGASVILFHVLEPPPDAVEVHATDALDCEIARQEARAYLDAISERVV
                     ERGVRVERHIAEGHPARGVAALATELDADLVVVSRFGEGGRDEWELGDTVQKILALAR
                     GAVLVVPPDARAPGPRVPPRRILVPLDGSLRGESVLPAALHLARTDDAELVLAHVVPD
                     PVRTAVLDTPEDLALAHELADRLVARAQAYLDGVCAELRRAGARVATVVTRAIDHREG
                     IVALAATREIDLVVVAAHGAVCNPRRRFGSVAAHLIEHAQAPVLVIQDLPLPSSHADA
                     DDAAPLPPRSSHGIRARA"
BASE COUNT          557 a         1375 c         1421 g          480 t
ORIGIN      
        1 gcatccgccg cggcgggcgt ccactccagc atgcgcttgc gcgtccacag catctggacc
       61 gcggcccgca ccacggcgtc gatcaccgtg cgggtctcct ggggcgcgaa gcagatcgcg
      121 agcatccagc ggccgaggtg acggcgcggc aggctggtgc tgcggaccag cgacgacacc
      181 gccgcggcgc cgggagccag cagcgcgatc agcgtccaca ccggcgggtg gccgggcagc
      241 acgagccacc cccacaccag cagcgcgagc accgcggggg cgaccaggct gcgccggagg
      301 ttgtcgagga tctggaaccg gccgagcgcg ccgaggtcgt tgcgccgggg ctcgccgcgc
      361 aacaccggca cgtgcggcag gagccaggga accagctgcc agtcgccgcg gatccagcgg
      421 tgcatccggc gcaggtacgc caggtagttc ggcgggtaat cttcatacac cacgacatcg
      481 ctggccagcg cgacccggcc ctggatcccc tcgaacaggt cgtggctcac cagcgcgttc
      541 tcggggacgc gcccggcgag gctggcgcgg aagctgtcga cgtcgtagat gcccttgccg
      601 acgtagatgc cctggccgaa cacgtcctgg tagacctcgg acacagcgcg gctgtagatg
      661 tcgaacccgg tatcgccggc gaacagccgg gaaaacgcgg agcgccgcgc gctccgtggt
      721 gagatctcga cccgcggctg gatcaccgtg tacccggcgc ggatccgccc gctcgcgtcg
      781 ggccgcggtc ggttgagcgg atgcgcgagc gttccggcga gccgggccgc ggtgccgggc
      841 ggcagctcgg tgtcgctgtc gagcgtgacg acgaacgcga tggcgtccag gccgcctggc
      901 tcgccggccg cgagcacgta gctcgtggcc gtgtcgcctc gcaggtagcg gttcagctcg
      961 tcgagcttgc cgcgcttgcg ctcccagccc atccacttgc cctcggccgg attccatcgc
     1021 ggcgcgcgat gcagcaggta gaacgggtgg cgagcgccgg tgccgtgccg gcggttgagg
     1081 cgatcgatcc cggccgcggc acgcgccagc tgggctcgct cgcgatcgcc gggtggctcg
     1141 tcgctgtcga ggtagtcggt cagcagcgcg aaccgcaact cggcgtccgg gttggcgagg
     1201 tagtacacct cgagctgcat cacgagccgg tcgatctccg cgtcgtcggt gagcatggtc
     1261 ggaaccgcga cgagcgtccg ccagcgcgcc gggatcccgc gcgagaaatc gagcttgggc
     1321 agcacccgtg gcgggatcag cgacgacagc accccatggg ccagcgtcgc cgccgggacc
     1381 gacgagggga cccagcacag cacgacggcg gccaccgccg aggccagcgg cgcgccgagc
     1441 caggccaggt agagcaccac cggcgcgagc agcacgagct gcgcggcggc gaggatgccg
     1501 aagaacgtca ggcgcggatg atcgagcaac cggcgcggca gcccccgccg gccggcaccc
     1561 aggctgcgct cgaacgggcg tcgccccgcg tcgaccagcc agtacccgac gtggtcgcag
     1621 cggccatcgg gctgcgccgc cgatgcgcga gcgaccaccg cgcgggcgac ttcgatctcg
     1681 tcgcggccgc tggcggtcgc gatgtcctcg atcacgcggc gaacctgatc gcgggtctcg
     1741 aactccatgg tcgcgtatgc ccgcgccgga tcgccctgga gcagctgctc gaccgcgctg
     1801 tgccgcgaga acacctcgcg ccagtcgacc tcggcgagca ggcgcaggcc gcggatcgca
     1861 cggccggcgg cgccgccgac atcctgcgac ggtgcgagcg cgtgcggctc gctcgccgcc
     1921 tgtgccagca gctcgtcgag gcggcgcagg atcgccagcc ggagcagcgc cggcagcgcc
     1981 cacagctcgg cgatcgtgag cggtgcgcgc tcctgatagt catcgaggaa gcggtcgagc
     2041 tcgtcgagat cgagctccgg catcgcgtcg aggatcacgc cggcgaccac cagggcgcgc
     2101 acccgcccat caccggccag gcgcggcaac cggcgctgga agctcgcggg gagctcgtgg
     2161 cggagctggg cgaacacgcc gcgcaccagg tagtcgttgt cgagcagcca ctcggtcgcg
     2221 atccgcaggg acgggttgtc gcgcggctgg gcgcgaagcc gcgtccggct gtgcgcgatc
     2281 gcggcctcca ggatgtccag gtcgctgcgg tcgaggaacg acgcgccggt cggtcggatc
     2341 tcgcgctgcg cctctgccag ctcgcgctcg gttcccggct catcgatcga tagggggctt
     2401 tcccgccccc tctggaccgg cgaagccggt ccattcaccc ctccgcgcgt cgcgtcgcgc
     2461 ggctcatcgc tgcgcggcgg gagcggatca cgcgcgagca cggatgccat ggctcgacct
     2521 cggaggcagc ggtgccgcgt cgtcggcatc ggcgtggctg gaaggcagcg ggagatcctg
     2581 gatcacgagc accggcgcct gggcatgctc gatcaggtgg gcggcgacgc tgccgaaccg
     2641 gcgcctggga ttgcagaccg cgccatgcgc ggccaccacc acgaggtcga tctcgcgggt
     2701 ggccgccagc gccacgatcc cttcgcgatg gtcgatggcg cgggtcacca ccgtggcgac
     2761 ccgggcgccg gcgcggcgca gctcggcgca gaccccgtcg agatacgcct gcgcccgtgc
     2821 gaccagtcga tcggcgagct cgtgcgccag ggcgaggtcc tcgggggtat cgaggaccgc
     2881 ggttcgcacc ggatccggga cgacgtgtgc gaggacgagc tcggcgtcgt cggtacgggc
     2941 gaggtggagc gctgcgggca gcacgctctc gccgcgcagc gacccgtcga gcggaaccag
     3001 gatcctccgc ggtggcacgc gcggcccggg agcacgcgcg tccggcggca ccacgagcac
     3061 ggcgccgcgc gccagcgcga ggatcttctg gacggtgtcg ccgagctccc attcatcgcg
     3121 tccgccctcg ccgaacctgg acaccacgac caggtcggca tcgagctcgg tggccagcgc
     3181 cgcgacgccg cgcgccgggt gtccttccgc gatgtggcgc tcgacccgca cgccgcgctc
     3241 gaccacgcgc tccgagatgg catccaggta ggcgcgggct tcctggcggg cgatctcgca
     3301 atcgagcgca tcggtcgcgt gaacctcgac cgcgtccggc gggggctcga gcacgtggaa
     3361 caggatcacg ctcgcgccat ccgccttcgc caggtgcgtg gcgagcggca gcgcagcttc
     3421 tgcagcgcgg gaccggtcga ggcagaccag caccgaccgg aacccggtcg gccgcggagc
     3481 gactgcgggc tcgtcgccga gggaggagtg ctgtccggcc atgcacgccg tcggtgcaat
     3541 gcacatgccc cgacgccgcg tacggcttcg cgtcctgatg gcccgaatgg cttgcgcaga
     3601 gtcagcctgc tcgcgcaccg catgcggcta tcggacgaca gatcagcagc tgggaatgga
     3661 acagcgcgcc ggcgcctggc aacttccgag ctgcgaggtc agtcggtgca agcaacgtcc
     3721 ggagcggcgg ctcggggccg gctgtccagg agcgccactt catcggcagg gcgcgtgcgc
     3781 gcgacgacga cggggcagcc ggcatggcgg accaccttct cggccaccga gcc
//
LOCUS       DASVOR010000567         6278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_63622,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000567 DASVOR010000000
VERSION     DASVOR010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63622"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2008)
                     /locus_tag="VF516_11215"
     CDS             complement(<1..2008)
                     /locus_tag="VF516_11215"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX8108291.1"
                     /translation="MSPRRYVVLAGSGVATFGAVMLGFWLSRVPALIELAAWRCQANT
                     CIGLLLGGIGLLAASSPRAVVRRIGAALAGAVTVFAALTLLEYAAGIDLEIDELIAKD
                     WPLPESATHPNRMAPHAALSFLLLGPALLLARRSRRLALVGQALAVAVIALDGVALIG
                     YVDGATFLYRVGTSMRISPYTALCFVVLGSGALALRPKLGVLARLSGDGIGTYLARRL
                     IIPVVVIPPVLDWLWLQGEIQGWWDRPTGLALDTLIYSGIAAALVLYLAHSLDVLDAR
                     RKRSELELQRIAELTAALAQARTVEEVARVTVERGVPALEAGAGAVFVIDPGGDQLRM
                     VASRGYPGEVSRTYATLPLTARAPICDAIRDRSMVFVATVDELRTRYPELTQVQPETR
                     AVAAVPLIATGRVLGALGLSFHARPPDEEARGRIDRLVGQCGQALDRAALFDSERAAR
                     EAARSADRTKDEFLAMLGHELRNPLAPITHALELMRLRNPQPHREREIVERQVRHMTR
                     LVDDLLDVSRIARGKIELTRSRVELAAVIDDAIELVRPLADERRHALIVQADPELAID
                     GDRARVVQIVSNLLTNAVKYTPSGGRIELTARRQGGDAVVRCTDTGVGIPASLLPHIF
                     EPFVQHGATIERAMGGLGLGLAIVRSLVRLHGGTAAVASAGEGKG"
     gene            complement(2086..2898)
                     /locus_tag="VF516_11220"
     CDS             complement(2086..2898)
                     /locus_tag="VF516_11220"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX8108292.1"
                     /translation="MTRLGDAIGLYGRYAAASVKSQLQYRASAVMTALGVFLITSSEF
                     AAVWALFDRFGQVRGWTLPEVALFYGVISITWALCDAISRGFDNFAQIVKSGEFDRIL
                     LRPRSTVLQLLGQELTLRRFGRLVQGALVLGYAFAAGTIDWTIGRAVLLVAAIACGIC
                     VFLGILVLQATSAFWTVESLEVWNSVTYGGLTTGQYPLAIYRSWLRALFTYVFPIGCA
                     TYFPGVAILGRADPLGTPPIVGWLAPLAGPVFLAACLQVWRIGVRHYRSTGS"
     gene            complement(2895..3734)
                     /locus_tag="VF516_11225"
     CDS             complement(2895..3734)
                     /locus_tag="VF516_11225"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX8108293.1"
                     /translation="MSAAVSPLAPYRALFGARFRMLLQYRAAALGGLVTQIAFGLILI
                     MIYEAFYRSSTAAAQPMAFGQIASYVWLGQALLMMLPWNVDNEMRAMMRTGGVAYELG
                     RPVDLYTWWFARALAQRTAPVMLRAAPMVVFAMLGLPLLGLGEWRLGPPASLSAGLGF
                     AAALAGALAVACAISVLLYIAWIWTIVAEGIVMVVSTAVSMFSGLLIPLPLFPDWARG
                     PLRWLPFAGVIDQPARIYAGHLASGELASVLVRQLGWAVVLIALGRRLLARGLRRVVV
                     QGG"
     gene            complement(3731..4744)
                     /locus_tag="VF516_11230"
     CDS             complement(3731..4744)
                     /locus_tag="VF516_11230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8108294.1"
                     /translation="MPQIVVEELVKTYQVAERTPGLWGAVRGLARRRHRTVRALDGVS
                     FAIERGELVGYIGPNGAGKSTTVKALSGILVPDSGRCEVAGRVPWKQRLDHVARIGVV
                     FGQRTQLWWDLPVVESFELLRDIYRVPPARFARSCEQLIALLDLAPLLDVPVRQLSLG
                     QRMRCDLTAALLHAPDILFLDEPTIGLDAVSKLAVREFIQRLNRDDGVTVILTTHDMD
                     DIEALCTRVMVIAGGKILSDGTLGELRRRVSRERWLTVDLARADAAIDDPEAQVIRRD
                     GARVWLAFDPEVVAPAELIRRVTSRCAIRDLFVENPPIETVVARLYAAHRLGEPGAAG
                     GAT"
     gene            complement(4829..>6278)
                     /locus_tag="VF516_11235"
     CDS             complement(4829..>6278)
                     /locus_tag="VF516_11235"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 47 protein"
                     /protein_id="HEX8108295.1"
                     /translation="IEDRTLLILRVDRKLSFDEIALVFAESLESVTEVEHSETGGGDP
                     DGSAGGAGGSAPRGIDRAAMAARVRAEVQRAWAGYVKYAWGHDELRPVTHTAHDWHGA
                     PLLITPVDALDTLVMLGLTAEADAARQLIDDRLSFDLDVTVKNFEITIRVLGGLLSGY
                     QLTGDRKLLWLADDLGARLLPVFDSPTGMPYMYVNLKTGQVSGARTNPAEIGTLLLEF
                     GALSKLTRKAVYYEKAKHAVVQLYERRSPLGLVGREIDVESGRWINPASHVGGGIDSY
                     YEYLLKGSLLFSDGQLAEMWRTSIAAVNQYLADEAGGLWYGQADMNSGRRIATRFGAL
                     HAFLPAVLALGGDLDRARRLEASCFRMWQLRGLEPELIDYRTMAIVAPEYELRPEIIE
                     SAWYLRRATGDPAYLAMGQAFLDGLVAHARTDAGFTVMTSVASMAQGDLMPSYFLAET
                     LKYLYLIFAPDDAVDLDRAVFTTEAHPLRPTW"
BASE COUNT          984 a         2373 c         2166 g          755 t
ORIGIN      
        1 cgcccttgcc ctcgcccgcg ctcgcgaccg ccgccgtccc gccgtgcagc cggaccaggc
       61 tgcgcacgat ggcgagcccg aggcccagcc cgcccatcgc gcgttcgatc gtcgcgccgt
      121 gctgcacgaa cggctcgaag atgtgcggca gcaggctggc cgggatcccg acgccggtgt
      181 cggtgcagcg cacgaccgcg tcaccgccct gccgccgcgc ggtcagctcg atccggccgc
      241 ccgacggcgt gtacttgacc gcgttggtca gcaggttgga cacgatctgg accacgcgcg
      301 cgcggtcgcc gtcgatcgcg agctccgggt cggcctgcac gatcagcgcg tgcctgcgct
      361 cgtccgccag cggccggacc agctcgatcg catcgtcgat caccgccgcg agctcgacgc
      421 gcgaccgcgt cagctcgatc ttgccgcgcg cgatccgcga gacgtcgagc aggtcgtcga
      481 ccagccgggt catgtgacgg acctggcgct ccacgatctc gcgctcgcgg tggggctgcg
      541 ggttgcggag ccgcatcagc tccagcgcgt gggtgatcgg ggcgagcggg ttgcgcagct
      601 cgtggccgag catcgccagg aactcgtcct tggtgcgatc ggcggagcgc gcggcctcgc
      661 gggcggcgcg ctcggagtcg aacagcgcgg cgcggtcgag cgcctggccg cactgcccga
      721 ccagccgatc gatgcgcccg cgggcctcct cgtcgggcgg ccgggcgtgg aagctcaggc
      781 cgagcgcgcc gagcacgcgc ccggtcgcga tcaggggcac cgccgcgacc gcgcgggtct
      841 cgggctggac ctgggtgagc tcggggtagc gcgtgcgcag ctcgtcgacc gtggcgacga
      901 ataccatgct gcgatcgcgg atcgcgtcgc agatcggcgc ccgggcggtg agcggcagcg
      961 tcgcgtacgt ccgcgacacc tcgcccgggt agccgcggct cgccaccatc cgcagctggt
     1021 cgccgcccgg atcgatcacg aacaccgctc cggcgcccgc ctcgagcgcc ggcacgccgc
     1081 gctcgaccgt gacccgagcg acctcctcca cggtgcgggc ctgtgcgagc gccgcggtca
     1141 gctcggcgat ccgctggagc tccagctcgc tgcgcttgcg acgcgcgtcg agcacgtcga
     1201 gcgagtgtgc caggtacagc acgagcgccg ccgcgatgcc gctgtagatc agcgtgtcga
     1261 gcgccagccc ggtgggcctg tcccaccagc cctggatctc gccctggagc cagagccagt
     1321 cgagcacggg cgggatcacc accaccggga tgatcaggcg gcgcgcgagg taggtaccga
     1381 tgccgtcgcc ggacagccgc gccagcacgc ctagcttggg acgcagcgcc agcgcgccgc
     1441 tgcccagcac gacgaagcac agcgcggtgt acggcgagat ccgcatcgat gtcccgacgc
     1501 ggtacaggaa cgtggcgccg tcgacgtaac cgatcagcgc gaccccgtcg agcgcgatca
     1561 ccgcgaccgc cagcgcctgg ccgaccaggg cgagccggcg tgaccgccgc gccagcagca
     1621 gcgccggacc gagcagcagg aacgacagcg cggcgtgcgg tgccatgcgg ttcgggtggg
     1681 tggcgctctc ggggagcggc cagtccttcg cgatcagctc gtcgatctcg agatcgattc
     1741 ccgctgcgta ctcgagcagc gtcagggccg cgaacacggt caccgcccct gcgagcgcag
     1801 ccccgatccg gcggaccacc gcgcgcggcg acgacgccgc cagcaggccg atgccgccga
     1861 gcagtaaccc gatgcacgtg ttggcctggc agcgccacgc cgcgagctcg atcagcgccg
     1921 gcacccgcga cagccagaag cccagcatca cggcgccgaa cgtggcgacg ccgctccccg
     1981 cgagaacaac gtaccggcgc ggagacatga ggcgaatcca tgcaaagtcg gctccccgac
     2041 cagctcttga tcatcgagaa cgaacgcgac cggtgcggca ccgcctcagc tgccggtcga
     2101 gcgataatgc cgtacgccga tccgccagac ctggaggcac gcggcgagaa acaccggccc
     2161 ggcgagcggc gcgagccagc cgacgatcgg cggcgtcccg agcggatcgg cgcggccgag
     2221 gatcgccacg cccgggaagt aggtggcgca gccgatcggg aacacgtacg tgaacagcgc
     2281 gcgcaaccac gagcgataga tcgccagcgg atactgcccc gtcgtcaggc cgccgtaggt
     2341 gaccgagttc catacctcga gcgactcgac ggtccagaac gccgaggtcg cctgcagcac
     2401 caggatcccg aggaacacgc agatcccgca cgcgatcgcg gcgaccagca gcacggcgcg
     2461 gccgatcgtc cagtcgatgg tccccgcggc gaacgcatag ccgagcacca gggcgccctg
     2521 caccagccgg ccgaaccggc gcagcgtcag ctcctggccg agcagctgga gcaccgtcga
     2581 gcgcggccgg agcaggatgc gatcgaactc gcccgacttg acgatctgcg cgaagttgtc
     2641 gaagccgcgc gagatcgcgt cgcacagcgc ccaggtgatc gagatgacgc cgtagaacag
     2701 cgcgacttcc ggcagcgtcc agccgcggac ctggccgaac cgatcgaaca gcgcccacac
     2761 cgcggcgaac tcgctgctgg tgatcaggaa cacgccgagc gcggtcatca ccgccgacgc
     2821 gcggtactgc agctggctct tgaccgacgc cgcggcgtag cggccgtaca gcccgatcgc
     2881 gtcgcccagg cgcgtcatcc gccctgcacc acgacgcggc gcaacccgcg agcgagcagc
     2941 cggcggccga gcgcgatcag cacgacggcc cagccgagct ggcgcacgag cacgctcgcc
     3001 agctcaccgg acgcgaggtg accggcgtag atccgcgccg gctggtcgat gacgccggcg
     3061 aacggcagcc agcgcagcgg cccgcgcgcc cagtcgggga acagcggcag cgggatcagc
     3121 agccccgaga acatcgacac cgccgtcgac acgaccatca cgatgccctc ggcgacgatc
     3181 gtccagatcc aggcgatgta gagcagcacc gagatcgcac aggccaccgc cagcgcgccg
     3241 gccagcgcgg ccgcgaaccc gagccccgcg gacagcgacg ccggcgggcc gagccgccac
     3301 tcgccgagcc cgagcagcgg caggcccagc atcgcgaaca cgaccatcgg cgccgcgcgc
     3361 agcatcaccg gcgcggtgcg ctgcgccagc gcgcgcgcga accaccacgt gtagaggtcg
     3421 accggccggc ccagctcgta cgccaccccg ccggtgcgca tcatcgcgcg catctcgttg
     3481 tcgacgttcc acggcagcat catcagcagc gcctggccga gccagacata ggacgcgatc
     3541 tgaccgaacg ccatcggctg cgccgcggcg gtcgacgagc gatagaacgc ctcgtagatc
     3601 atgatcagga tcaggccgaa cgcgatctgg gtgaccagcc cgccgagcgc cgcggcgcgg
     3661 tactgcagca gcatgcgaaa ccgcgcgccg aacagcgcgc gatagggcgc gagcgggctc
     3721 accgcggcgc tcacgtcgcg cctccggccg cgccgggctc gccgagccga tgcgccgcgt
     3781 acagccgcgc gaccacggtc tcgatcggcg ggttctcgac gaacaggtcg cggatcgcgc
     3841 accggctggt cacgcggcgg atcagctcgg ccggcgcgac gacctcgggg tcgaacgcca
     3901 gccacacccg ggcgccgtcg cgccggatca cctgggcctc gggatcgtcg atcgccgcgt
     3961 cggcgcgcgc gaggtcgacg gtcagccagc gctcgcggct gacccggcgg cgcagctcgc
     4021 cgagcgtgcc gtccgacagg atcttgccgc cggcgatcac catcacccgc gtgcacagcg
     4081 cctcgatgtc gtccatgtcg tgcgtggtca ggatcaccgt cacgccgtcg tcgcggttga
     4141 gccgctggat gaactcgcgc accgcgagct tggacaccgc gtcgagcccg atcgtcggct
     4201 cgtcgaggaa caggatgtcg ggcgcgtgga gcagcgccgc cgtcagatcg caccgcatgc
     4261 gctggccgag cgacagctgg cgcaccggca cgtcgagcag cggcgcgaga tcgagcagcg
     4321 cgatcagctg ctcgcacgac cgcgcgaacc gcgccggcgg cacgcggtag atgtcgcgca
     4381 gcagctcgaa cgactcgacc accggcaggt cccaccacag ctgggtgcgc tggccgaaca
     4441 ccacgccgat ccgcgcgacg tggtcgagcc gctgcttcca cggcacgcgc ccggcgacct
     4501 cgcagcgccc gctgtccggc accaggatcc ccgacagcgc cttgaccgtc gtgctcttgc
     4561 cggcgccgtt gggcccgatg tagccgacca gctcgccgcg ctcgatcgcg aacgacaccc
     4621 cgtcgagcgc ccgcaccgtg cgatgccggc gccgcgccag cccgcgcacg gcgccccaca
     4681 gccccggcgt gcgctcggcg acctggtagg tcttgaccag ctcttcgacg acgatctgcg
     4741 gcacgggccc ccaggctgcc gcgttccgcg atcgcgcgcc agccgcacgc ccggcattcc
     4801 tcggcgcgcc gcgcgcgaca gactgcgact accaggtcgg tcgcagcggg tgcgcctcgg
     4861 tcgtgaacac cgcgcgatcg agatcgaccg cgtcgtcggg cgcgaagatc aggtacaggt
     4921 acttgagggt ctcggcgagg aagtagctcg gcatcaggtc gccctgcgcc atggacgcga
     4981 cgctggtcat caccgtgaac ccggcgtcgg tgcgggcgtg ggcgaccaga ccgtcgagga
     5041 acgcctggcc catcgcgagg tacgccggat cgccggtcgc gcgccgcagg taccacgccg
     5101 actcgatgat ctcgggccgc agctcgtact cgggcgcgac gatcgccatc gtgcggtagt
     5161 cgatcagctc gggctcgagc ccgcgcagct gccacatccg gaagcacgac gcctcgagcc
     5221 ggcgcgcccg gtcgagatcg ccgccgagcg ccagcaccgc cgggagaaac gcgtgcagcg
     5281 cgccgaaccg ggtcgcgatc cggcgccccg agttcatgtc ggcctgtccg taccacagcc
     5341 cgccggcctc gtcggcgagg tactggttca ccgccgcgat gctggtccgc cacatctccg
     5401 cgagctggcc gtcgctgaac aggagcgagc ccttgagcag gtactcgtag tacgagtcga
     5461 tcccgccgcc gacgtggctc gccgggttga tccaccgccc cgactcgacg tcgatctccc
     5521 ggccgaccag cccgagcggc gagcgccgct cgtagagctg caccaccgcg tgcttcgcct
     5581 tctcgtagta gaccgccttg cgggtcagct tcgacagcgc cccgaactcc agcagcagcg
     5641 tgccgatctc cgccgggttg gtccgcgcgc ccgacacctg cccggtcttc aggttgacgt
     5701 acatgtacgg catcccggtc ggcgaatcga acaccggcag gagccgcgcg ccgaggtcgt
     5761 cggccagcca cagcagcttg cgatctccgg tcagctggta gcccgacagc aggccgccga
     5821 gcacccggat cgtgatctcg aagttcttga ccgtgacgtc gaggtcgaac gacagccggt
     5881 cgtcgatcag ctggcgcgcg gcgtcggcct cggcggtcag cccgagcatc accagcgtgt
     5941 cgagcgcatc gaccggcgtg atcaggagcg gcgcgccgtg ccagtcgtgc gcggtgtggg
     6001 tcaccggcct gagctcgtcg tgcccccagg cgtacttcac gtagcccgcc cacgcgcgct
     6061 gcacctccgc ccgcacccgc gccgccatcg ccgcgcgatc gatcccacgg ggggcgctgc
     6121 cccccgcccc gccggcggag ccgtcggggt ccccaccccc cgtctcgctg tgctcgacct
     6181 cggtcaccga ctcgaggctc tcggcgaaca ccagcgcgat ctcatcgaag ctgagcttgc
     6241 ggtcgacgcg caggatcagc agggtgcggt cctcgatc
//
LOCUS       DASVOR010000568        11410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443437,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000568 DASVOR010000000
VERSION     DASVOR010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443437"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1591)
                     /locus_tag="VF516_11240"
     CDS             complement(<1..1591)
                     /locus_tag="VF516_11240"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HEX8108296.1"
                     /translation="MFAAIAIAAAAGLAVAQTAAPRHKLHEDLPSPSDKPTRMIGGTG
                     SGNPAAFTAGDKVLPRPALDRPSGRPADEPVLGNGGFAADRRTTMTPDGNTGPDATLH
                     YVSVFNPDVLPFKRMSALDGVADDYTLKIAHTALVDLPVGGATDKTRDPFWGSVLLHL
                     TPGQDVPLPSVAPDMRILSYEITPKIAVKFEKDGADNFYVRSDESSAAGTYRLVFAAD
                     ADAGYFAPSLPTTGRYTPLMSAAMTPPELRPALPDRIRRTARQVLDRLGVDAEMDLGV
                     VFNRLVGYFRAFQAKPLHASSSDIYLDLCFSQAGVCRHRAFAFMITANALGIPTRFVE
                     NEAHAFVEVWFPDRRWQRIDLGGAALRMEVTGASDKVLHRPRADDPFEKPPEYKQNYT
                     QLEGDIKGLTRQQLADKHRSLNQAPPSGAFDPGLGAGPGSGAGAIPDRILPDQSLPAV
                     TRDPKKASPSLSVTFVDDRAFRGSELHVEGHATAASKPLPDHTVDVFLSPAGRGGSGS
                     IFLGRAVTEADGSFRAYFPVPP"
     gene            1671..2939
                     /locus_tag="VF516_11245"
     CDS             1671..2939
                     /locus_tag="VF516_11245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8108297.1"
                     /translation="MDSAARRTINLAVLVAALGYFVDIYDLILFSMVRPRSVADLGVP
                     PQEILPQGIHLFNMQMGGLLVGGILWGVLGDKRGRLSVLFGSITMYSLANIANGLVHS
                     VEGYAACRLIAGIGLAGELGAGVTLVSELMGRHNRGLGTALVAGLGILGGLLAGWIGG
                     AFGPGLPWRVAFFIGGGLGIALLVLRIGVAESGLFRSTAARKQIARGNFFQLFQRKRL
                     GRYLAIIAAGVPIWYVVGILYTFSNDLGKALGLAEPPIPARVLFFDYGGAAIGGVLSG
                     VLSQYLQSRRRAMAIFGIGLAVMVAAYFLVGGMSSTLFYALCGLGGVFTGYWAVFMST
                     ATELFGTNLRATVAITAPNFVRGSAVLLALGFQYLTPALGLVGAALAVGVFAFGVGAI
                     GLASVRETFHVDLDFHEVDDAALPAASAAT"
     gene            complement(2923..3300)
                     /locus_tag="VF516_11250"
     CDS             complement(2923..3300)
                     /locus_tag="VF516_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108298.1"
                     /translation="MGPGGSHLDAAFSLRAAVPAGTYHVECDAVITASIDVTFSLIWR
                     RGATDMMLAQWMKHWDPLVGSFNAQAYEVDMAAPAIDYQPGDQLVFRYAGNGPPNVLA
                     NAFIPNGDGRNSNGRIPAITLPH"
     gene            complement(3310..3471)
                     /locus_tag="VF516_11255"
     CDS             complement(3310..3471)
                     /locus_tag="VF516_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108299.1"
                     /translation="MMRISLAFLALGMLALPGCPDPEPTGNPTTLWLSSIGNDETHVQ
                     LVATQPPPF"
     gene            complement(3468..4130)
                     /locus_tag="VF516_11260"
     CDS             complement(3468..4130)
                     /locus_tag="VF516_11260"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8108300.1"
                     /translation="MRALILVLLVAASCGHREAEGDSASGSPGSGPSATNDSAREQTR
                     FDAERRPEKVVEALGIGPGSRVADIGAGSGLLTVHLARAVRPNGRVVATDIDRAVLDL
                     LQSRLAAAGLADLVDTKVVAAEETGLAPASYDAILLAEVDHYFSDEVAWLKEATKALK
                     PGGRIVISNRIHHRPRSMEAARRAGLKLVSESTPVPTHFIAVFTGPRGPGAPGAGTAG
                     GT"
     gene            complement(4236..5633)
                     /locus_tag="VF516_11265"
     CDS             complement(4236..5633)
                     /locus_tag="VF516_11265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108301.1"
                     /translation="MAKTPLSKRDEKHELTRRALIKWSIAAGAALGVSRSKVYEILER
                     TAGKDVAFAASAQLINRTFDIEFGNGSLSWMTQFWPFPAIAAAATPTNSLSWAFPGQQ
                     TVVQGTDRPLTIGPQTPWAKLPANKQVTAFLCGNNETHTNFAQSTNSISGNSLLASNA
                     ALQAALPAIIPTVTVGQGTTIGTAPGATTAANVANAAGVVDLFNSVASRAGGLLANAT
                     DAGHYKTFYEGFIQLNRAANRPTTNIGYTTAASAAQFLGTNLSSKLSVTPADEARYGI
                     TGATRSTVKALGDAMIVAVKAFGLGLTNGFVVPAMRDDPHGAFDNNDVAVVPAQLKGV
                     FDGFMTDLQNTLDPVAQTPIANNIVITMKGDTFKSPTNKAGWGDGTPMNTNLVYVYSA
                     GQLMSGWFGDISATGTAQGVGADGKLTTYNGTNTAKYALASVLYATAIGDDRAIQNLA
                     SGVQVSGIFGRVKQI"
     gene            complement(5645..6331)
                     /locus_tag="VF516_11270"
     CDS             complement(5645..6331)
                     /locus_tag="VF516_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108302.1"
                     /translation="MTRFGYTLTILFGASLGVAACTDNIDTGNTPTDQPPSGSTTGSD
                     TNTFDHDNSGISVWDLIDRLTKEGPPSFSSQMHGCVKPTYATLKNILTSVGINAANTA
                     NPSAGQLYTSGAAAMGAPNFSARVKENMSVSTSGASREFDIFAAGATEVMTNLPNLAR
                     CKDASGAAAVLFDASGQCQASGITCLIGVPATPDHVALCNQTVTTASTPDIGKRMAVA
                     AMLAAAYTCQ"
     gene            complement(6342..7547)
                     /locus_tag="VF516_11275"
     CDS             complement(6342..7547)
                     /locus_tag="VF516_11275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108303.1"
                     /translation="MAATSFAVPACSSDVTPNGGDDGSGSNTGSNTGSNTGSNQGSGD
                     QWDQILANRVTDYNAALRAAALRLTGDLPSMTEINTVASATGDAQKTAYETLIKDYMG
                     RPTFANQMFLFWRDTFKTGGATAAFDAGKAPAPSPDTAAAFAAEIAVNNGSYMDLFTK
                     ASANCPTFAPATGTFTNAECTNNGPKAGVLTNPGLMSQFFSNLSMRRGRFVQETFDCL
                     RFPVEISTTPTDVGGSAPYIGVWPFTSIASPTNGNGRVNFQDVSAAICANCHQTLNHL
                     VPLFAYYDANGNYQTTIQVPVPLPGNPTAQITDWLPPGETTAWRYQKPAADIPALGQQ
                     MAADPSIAQCGVARLWNMALGKEDIVDQLVSVPAATIKAQTDAFTANGFKVKDLLFSI
                     FTDKAFVSF"
     gene            complement(7582..8790)
                     /locus_tag="VF516_11280"
     CDS             complement(7582..8790)
                     /locus_tag="VF516_11280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108304.1"
                     /translation="MLETLCRHLDMVRLSAAGVLMVALVGCTGLVDGGSDGLTPQQKT
                     ARQMWEQKAIPVLRQDCLTCHNGSRASVGFLIGDDDLSIHDTLMAFQPAVVNLDAASS
                     SQIVTKGLHEGPQLTDGELSNMLDWLFAERDASNHDPDHPIAQLATKPAAIQLCTGGD
                     PDNAAGTCPTNHVSLTGIPTDGTMLAGAELAFTAQPLGSGIYLSNMVASGGTSGFYTE
                     HLLFVSVPAKTAPFPDQLDRYFALKLNVGPSKTSPIDGGTADFVGFVPTDMLEIHFKV
                     LTAFKPDTTGTMPDGCKVLDKFTASAMPELTKNCTSCHGGANPTAVSSMDLTTIGTAT
                     TDNTKILAACNQVRSRINFQTPDQSGFYIAPNTGSNHPFKFPNPTSGETFTTFKTAMD
                     VWVTAEKTAP"
     gene            complement(9105..10016)
                     /locus_tag="VF516_11285"
     CDS             complement(9105..10016)
                     /locus_tag="VF516_11285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8108305.1"
                     /translation="MSNLASSTTDTAQASNLILYAIPAFVIAVVAEALWARRHRDDDR
                     IRGYELRDSAASMTMGLLNVVVSGFTKLLSIPFFAWLYQHRVADIGTAWWTWIVLLLG
                     EDLCYYWFHRVHHEVRLLWACHVNHHSSQRFNLSTALRQPLLTPLSGPIFWAPLPLIG
                     FPPWMVLTAQAWSLFYQFWIHTEAVDRLGPLEWFMNTPSHHRVHHGKNVPYLDKNHGG
                     IFIVWDRLFGTFAPEAEHVAYGLTKDIQTYNPLRIGFHEFAAIGRDVVRAPGLGAKLG
                     YLLAPPGWSHDGSSLTATQLQRRRSAH"
     gene            10082..>11410
                     /locus_tag="VF516_11290"
     CDS             10082..>11410
                     /locus_tag="VF516_11290"
                     /inference="COORDINATES: protein motif:HMM:NF012431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8108306.1"
                     /translation="MTHGGDIIADVLARHGVTHLFTLCGGHISPILTGAQARGLRVVD
                     VRDEANAVFAADAVARMTGTVGVAAVTAGPGVTNAVTAVKNAQMAQSPVLLLGGATAT
                     LLKGRGSLQDIDQMAVMKPLTKWATAVKTVPALAPVIERACQIATEGVPGPVFVEVPV
                     DLLYPEATVREWYAREAGLGRGKGIGAKAVELYIRGHLYRQFRAPAMPEVHVPVKLPS
                     LFSDGGVERAAEAIKTAQRPVFVIGSQALVGVKDPGRLARSLGMLGAPVFLGGMARGL
                     LGRSHELQLRHNRGAALKRADVIVVCGFPFDFRLGYGRGLNRDATLIAANLSPQELRK
                     NRRPDIAVEMHAGEFVIALADRISSAGDRWRGWLDELRAQERARDAEIAARAQPAGEL
                     VDPLHFFLRLEEAMADDAVLVVDGGDFVATASYIVRPRAPLSWLDPGAFGT"
BASE COUNT         1564 a         3674 c         4299 g         1873 t
ORIGIN      
        1 tcggcggcac cgggaagtag gcgcggaacg acccgtcggc ctcggtgacc gcgcggccca
       61 ggaagatcga gcccgagccg ccgcgcccgg cgggcgacag gaacacgtcg acggtgtggt
      121 cggggagcgg cttgctggcg gccgtcgcgt ggccctcgac gtggagctcg gagccgcgga
      181 acgcgcggtc gtcgacgaag gtcacgctca acgacggcga cgctttcttg ggatcgcggg
      241 tcaccgcggg cagcgactgg tcgggcaaga tgcgatcggg gatggcgccg gcgccggagc
      301 cagggccggc gccgaggccc ggatcgaacg caccgctcgg cggtgcctgg ttcagcgagc
      361 ggtgcttgtc ggcgagctgc tgccgggtca ggcccttgat gtcgccctcg agctgcgtgt
      421 agttctgctt gtactcgggc ggcttctcga acgggtcgtc ggcgcgcggc cggtgcagga
      481 ccttgtcgct ggcgccggtg acctccatgc gcagcgcggc gccgcccaga tcgatccgct
      541 gccagcgccg gtcggggaac cagacctcga cgaaggcgtg ggcctcgttc tcgacgaacc
      601 gggtcgggat gccgagcgcg ttggcggtga tcatgaacgc gaacgcgcgg tggcggcaca
      661 cgccggcctg gctgaagcac aggtcgaggt agatgtcgct cgacgacgcg tgcagcggct
      721 tggcctggaa cgcgcggaag tagccgacca gccggttgaa caccacgccg aggtccatct
      781 cggcgtcgac gccgagccgg tcgagcacct ggcgggcggt gcggcggatc cggtcgggca
      841 gggccgggcg gagctcgggc ggggtcatcg cggcgctcat cagcggcgtg tagcggccgg
      901 tggtcggcag cgacggcgcg aagtagccgg cgtcggcgtc ggccgcgaac accagccggt
      961 aggtgccggc ggcgctcgac tcgtcgctgc ggacgtagaa gttgtcggcg ccgtccttct
     1021 cgaacttgac cgcgatcttc ggcgtgatct cgtacgacag gatgcgcatg tcgggcgcga
     1081 cgctgggcag cggcacgtcc tggccgggcg tgaggtggag cagcaccgag ccccagaacg
     1141 gatcgcgggt cttgtcggtc gcgccgccga ccggcaggtc gaccagcgcg gtgtgcgcga
     1201 tcttgagcgt gtagtcgtcg gcgacgccgt cgagcgcgct catccgcttg aacggcagca
     1261 cgtcggggtt gaacacgctg acgtagtgca gcgtggcgtc ggggccggtg ttgccgtcgg
     1321 gcgtcatcgt ggtccgccgg tcggccgcga acccgccgtt gccgagcacc ggctcgtcgg
     1381 ccgggcgacc gctcggtcgg tcgagcgccg gcctgggcag caccttgtcg cccgcggtga
     1441 acgccgccgg gttgccgctg ccggtgcccc cgatcatccg ggtcggcttg tcggacggcg
     1501 acggcaggtc ctcgtggagc ttgtggcgcg gtgcggcggt ctgggcgacg gccaggccgg
     1561 cggcggcggc gatcgcgatg gcggcgaaca tcggcccggg gcgcatcgca tggataatga
     1621 cacggcgcag ccggtgcgca ccggattgca gtccgcgtat agtccggccc gtggactccg
     1681 cggcacggcg tacgatcaac ctggccgtcc tggtggcggc gctcgggtac ttcgtcgaca
     1741 tctacgacct gatcctgttc tcgatggtcc ggccgcgcag cgtggcggac ctcggcgttc
     1801 cgccgcagga gatccttccg cagggcatcc acctgttcaa catgcagatg ggcgggctgc
     1861 tcgtcggcgg catcctgtgg ggcgtgctcg gcgacaagcg cggccggctg tcggtgctgt
     1921 tcggctcgat cacgatgtac tcgctggcca acatcgccaa cgggctcgtg cacagcgtcg
     1981 agggctacgc cgcgtgccgg ctgatcgcgg ggatcggcct ggcgggcgag ctcggcgccg
     2041 gggtcacgct ggtcagcgag ctgatgggcc ggcacaaccg cgggctcggc acggcgctcg
     2101 tggcggggct cggcatcctc ggcgggctgc tggcgggctg gatcggcggc gcgttcggcc
     2161 cggggctgcc gtggcgggtc gcgtttttca tcggcggtgg cctcgggatc gcgctgctcg
     2221 tgctgcggat cggcgtcgcc gagtccggtc tgttccgctc caccgccgcg cggaagcaga
     2281 tcgcgcgcgg caacttcttc cagctgttcc agcgcaagcg gctggggcgc tacctcgcga
     2341 tcatcgccgc cggcgtgccg atctggtacg tggtcggcat cctgtacacg ttctccaacg
     2401 acctgggcaa ggcgctcggg ctggccgagc cgcccatccc ggcgcgcgtg ctgttcttcg
     2461 actacggcgg cgcggcgatc ggcggcgtcc tgtccggggt gctcagccag tacctgcagt
     2521 cgcgacggcg cgcgatggcg atcttcggga tcgggctcgc ggtgatggtc gcggcgtact
     2581 tcctggtcgg cgggatgtcg agcacgctgt tctacgcgct gtgcggcctc ggcggggtct
     2641 tcaccggcta ctgggcggtg ttcatgtcga cggcgaccga gctgttcggc accaacctgc
     2701 gcgccacggt ggcgatcacc gcgccgaact tcgtgcgcgg ctcggccgtg ctgctcgccc
     2761 tcgggttcca gtacctcacg ccggcgctcg gcctggtcgg tgcggcgctc gcggtcggcg
     2821 tgttcgcgtt cggcgtcggg gcgatcggcc ttgcgtcggt gcgcgagacg ttccacgtcg
     2881 acctcgactt ccacgaggtc gacgacgccg cgctgcccgc ggctagtgcg gcaacgtgat
     2941 cgcggggatc cggccgttcg agttgcggcc gtcgccgttg gggatgaacg cgttggcgag
     3001 cacgttgggc gggccgttgc cggcgtagcg gaacacgagc tgatcgccgg gctggtagtc
     3061 gatcgccggc gcggccatgt cgacctcgta ggcctgggcg ttgaagctgc cgaccagcgg
     3121 gtcccagtgc ttcatccact gcgcgagcat catgtcggtc gcgccgcgcc gccagatcag
     3181 cgagaacgtg acgtcgatcg acgcggtgat caccgcatcg cactcgacgt ggtacgtgcc
     3241 ggccgggacg gcggcgcgca gcgagaacgc ggcgtcgaga tgggacccac cgggccccac
     3301 gccgggcgct caaaacggcg gcggctgggt ggcgaccagc tggacgtggg tctcgtcgtt
     3361 gccgatcgat gacagccaca gcgtcgtggg gttgcccgtg ggctccgggt cgggacagcc
     3421 gggcagtgcg agcatgccga gcgccaggaa ggcgagggag atccgcatca tgtacctccg
     3481 gcggtgcccg ccccgggagc cccgggaccc cggggacctg tgaacaccgc gatgaagtga
     3541 gtcggcaccg gcgtggattc ggacaccagc ttgagcccgg cgcgccgggc cgcttccatc
     3601 gatcgcgggc ggtggtggat ccggttggag atcacgatcc ggccgcccgg cttgagcgcc
     3661 ttggtcgcct ccttgagcca ggcgacctcg tcggagaaat agtgatcgac ctcggcgagc
     3721 aggatcgcgt cgtagctcgc gggggccagc cctgtctcct cggcggcgac caccttggtg
     3781 tcgaccagat cggcgaggcc ggcggcggcg agccgcgact gcaggagatc gagcaccgcg
     3841 cggtcgatat cggtggcgac cacgcggccg ttgggccgga ccgcgcgggc caggtgcacg
     3901 gtcaggaggc ccgagccggc gccgatgtcg gcgacccggg agcccgggcc gatgccgagg
     3961 gcctcgacca ccttctcggg ccgccgctcg gcgtcgaacc gggtctgctc gcgcgcggag
     4021 tcgttggtcg ccgacggccc cgagccgggg ctgccggagg cagaatcccc ttccgcctcg
     4081 cggtggccgc agctcgccgc gacgagcaag accaggatca gcgcacgcac gccggtagcc
     4141 tagcacgcgc tcgccgggcg gccgcgccgt gcacagaacg taacgaggcc gccgagcgag
     4201 cccggcggcc tccgtcacgg tccagcgccg atcgactaga tctgcttgac ccggccgaag
     4261 atgccggaca cctggacgcc gctggcgagg ttctggatcg cgcggtcgtc gccgatcgcg
     4321 gtcgcgtaca gcaccgaggc cagcgcgtac ttggcggtgt tcgtgccgtt gtacgtcgtc
     4381 agcttgccgt cggcgccgac gccttgtgcc gtgccggtgg cgctgatatc gccgaaccag
     4441 ccgctcatca gctggcccgc gctgtagacg tacaccaggt tggtgttcat cggcgtgccg
     4501 tcgccccagc cggccttgtt ggtcgggctc ttgaaggtgt cgcccttcat cgtgatcacg
     4561 atgttgttgg cgatcggcgt ctgggcgacg gggtcgagcg tgttctgcag gtcggtcatg
     4621 aacccgtcga acacgccctt gagctgggcc gggaccaccg cgacgtcgtt gttgtcgaac
     4681 gcgccgtgcg gatcgtcgcg catcgccggg acgacgaagc cgttggtcag gccgagcccg
     4741 aatgccttga ccgcgacgat catcgcgtcg ccgagcgcct tcaccgtgct gcgcgtcgcg
     4801 ccggtgatgc cgtagcgggc ctcgtcggcc ggcgtcaccg agagcttcga cgacaggttg
     4861 gtgccgagga actgcgccgc gctggccgcc gtggtgtagc cgatgttggt ggtcgggcgg
     4921 ttcgccgcgc ggttgagctg gatgaagccc tcgtagaacg tcttgtagtg cccggcatcc
     4981 gtcgcgttgg cgagcaggcc gccggcgcgc gacgcgacgc tgttgaacag gtcgacgacg
     5041 ccggccgcgt tggcgacgtt ggccgcggtg gtcgcgcccg gcgcggtgcc gatcgtcgtg
     5101 ccctggccga cggtgaccgt cgggatgatg gcgggcagcg cggcctgcag cgcggcgttg
     5161 ctcgcgagca ggctgttacc gctgatcgag ttggtcgact gcgcgaagtt ggtgtgggtc
     5221 tcgttgttgc cgcacaggaa cgcggtgacc tgcttgttcg ccggcagctt ggcccacggg
     5281 gtctgggggc cgatcgtcag cggacgatcc gtgccctgca cgaccgtctg ctggccgggg
     5341 aaggcccacg acagcgagtt ggtcggcgtc gcggcggccg cgatcgcggg gaacggccag
     5401 aactgcgtca tccacgacag gctgccgttg ccgaactcga tgtcgaacgt ccggttgatc
     5461 agctgggcgc tcgccgcgaa cgcgacgtcc ttgcctgcgg tgcgctccag gatctcgtag
     5521 accttgctgc gcgacacgcc gagggccgca ccggccgcga tgctccactt gatcaaagca
     5581 cggcgcgtca gctcatgctt ctcatcacgc ttggaaagag gggtcttggc catggtagag
     5641 ctcctcactg gcaggtgtag gccgcagcca gcatggccgc gacagccatg cgcttgccga
     5701 tgtcgggggt ggacgcggtc gtgacggtct ggttgcacag ggcaacgtga tccggggtcg
     5761 ccggcacgcc gatgaggcag gtgatgccgc ttgcctggca ctgcccgctt gcgtcgaaca
     5821 gcaccgcggc ggcgccgctg gcgtccttgc agcgcgccag gttcggcagg ttggtcatga
     5881 cctccgtggc gccggccgcg aagatgtcga actcgcgcga cgcgccggag gtgctgaccg
     5941 acatgttctc cttgacccgg gcgctgaagt tcggcgcgcc catcgccgcg gcgcccgagg
     6001 tgtagagctg gccggccgac gggttcgccg tgttggcggc gttgatgccg accgaggtca
     6061 ggatgttctt cagcgtggcg taggtcggct tgacgcagcc gtgcatctgc gacgagaagc
     6121 tcggcggccc ctccttggtg agccggtcga tcaggtccca caccgagatg ccgctgttgt
     6181 cgtgatcgaa ggtgttggtg tcgctgccgg tcgtgctgcc ggacggcggc tgatcggtcg
     6241 gggtgttgcc ggtgtcgatg ttatccgtgc acgcggccac accgagcgac gcgccaaaca
     6301 ggatcgtgag ggtgtatcca aagcgggtca ttggcatctc ctcagaacga gacgaaggcc
     6361 ttgtcggtga agatcgagaa cagcagatct ttcaccttga agccgtttgc ggtgaatgcg
     6421 tcggtttggg ccttgatcgt cgccgccggg acactgacca gctgatcgac gatgtcttcc
     6481 ttgccgaggg ccatgttcca gagccgcgcg acgccgcact gcgcgatgct cgggtcggcc
     6541 gccatctgct gaccgagcgc ggggatgtcg gcggcgggct tctgatagcg ccacgcggtg
     6601 gtctcgccgg gcggcagcca gtcggtgatc tgcgcggtcg gattacccgg gagcgggacc
     6661 ggcacctgga tcgtcgtctg gtagttcccg ttcgcgtcgt agtacgcgaa cagcggcacg
     6721 aggtggttga gcgtctgatg gcagttggcg cagatcgcgg ccgacacgtc ctggaagttg
     6781 acccggccgt tgccgttggt cgggctggcg atgctggtga acggccacac gccgatgtac
     6841 ggcgccgagc cgccgacgtc ggtcggcgtg gtcgagatct cgaccgggaa ccgcaggcag
     6901 tcgaacgtct cctggacgaa ccggccacgg cgcatcgaca ggttcgagaa gaactggctc
     6961 atcaggccgg ggttggtcag cacgccggcc ttgggaccgt tgttggtgca ctcggcgttg
     7021 gtgaacgtgc cggtcgccgg cgcgaacgtc gggcagttcg cggacgcctt ggtgaacagg
     7081 tccatgtagc tgccgttgtt gaccgcgatc tcggcggcga acgctgcggc ggtgtcggga
     7141 ctcggtgcgg gagctttgcc ggcgtcgaac gctgcggtcg cgccaccggt cttgaacgtg
     7201 tcgcgccaga acaagaacat ctggttggcg aacgtcggcc ggcccatgta gtccttgatc
     7261 agggtctcgt acgcggtctt ctgcgcgtca ccggtcgccg acgcgaccgt gttgatctcg
     7321 gtcatcgagg gcaggtcgcc ggtcaaccgc agtgcggcgg cgcgcagcgc ggcgttgtag
     7381 tcggtgaccc ggttggccag gatctggtcc cactggtcgc ccgaaccctg gttcgagccc
     7441 gtattggagc ccgtgttcga gcccgtgttc gagccagagc cgtcgtcgcc accgttgggg
     7501 gtgacgtcgc tgctgcatgc cgggaccgcg aaagatgtcg cggccaagag cagaattaag
     7561 atcctcttca tggggctcct tttagggggc ggtcttttcg gccgtgaccc agacgtccat
     7621 ggcggtcttg aaggtcgtga aggtttcgcc gctcgtcggg ttcgggaact tgaacgggtg
     7681 gttgctgccg gtgttgggag cgatgtagaa cccgctctgg tcgggggtct ggaagttgat
     7741 ccgcgaccgg acctggttgc aggcggccag gatcttggtg ttgtcggtgg tggcggtccc
     7801 gatcgtggtc agatccatcg aggacaccgc ggtcgggttg gcaccgccgt ggcagctggt
     7861 gcagttcttc gtgagctcgg gcatcgcgct ggccgtgaac ttgtccagca ccttgcagcc
     7921 gtccggcatc gtgccggtgg tgtcgggctt gaacgcggtc agcaccttga agtggatctc
     7981 gagcatgtcg gtcgggacga agccgacgaa gtccgcggtg ccgccgtcga tcgggctggt
     8041 cttgctcggt ccgacgttga gcttgagcgc gaagtagcga tcgagctggt cggggaacgg
     8101 ggcggtcttg gcgggcaccg agacgaacag caggtgctcg gtgtagaacc cggaggtccc
     8161 gccgctggcg accatgttgc tgaggtagat gccggagccc aggggctggg cggtgaacgc
     8221 gagctcggcg ccggcgagca tggtgccgtc ggtcgggatg ccggtcagcg acacgtggtt
     8281 ggtcgggcag gtgccggcgg cgttgtcggg gtcaccgccg gtgcacagct ggatcgcggc
     8341 cggcttggtc gcgagctgcg cgatcgggtg atcgggatcg tggttggacg cgtcgcgctc
     8401 ggcgaacagc cagtcgagca tgttcgacag ctcgccgtcg gtcagctgcg ggccctcgtg
     8461 caggcccttg gtgacgatct gcgagctgct cgcggcgtcg aggttgacca ccgccggctg
     8521 gaacgccatc agcgtgtcgt ggatcgagag atcgtcgtcg ccgatcagga agccgacgct
     8581 ggcgcgcgag ccgttgtggc aggtcaagca gtcctggcgc agcaccggga tcgccttctg
     8641 ctcccacatc tggcgggcgg tcttctgctg gggcgtcagg ccgtcgcttc caccgtcgac
     8701 cagcccggtg cagccgacca gggccaccat caggacgccg gccgccgaca gccgcaccat
     8761 gtccaggtga cggcacaggg tctccagcat cgccctgcgc gactgcagag gggtgccgag
     8821 gttcttctga acagaaggaa cgctacccag catgcggcgg acaaacagca acgcgtatgc
     8881 cggctgggat tgcgccgagc ggcgggctat ctaccgggtt ttcttagatg gggacaaatg
     8941 tgtacctcca ttgcgcatga ggtttcacac cccacctggg gacccgcatg cccaggttgt
     9001 aacaccgcga aaccgcgtgg gcgtgttgtg cagcaatcgc cgcttgccgg gagggaaggg
     9061 ttggcgaggt gcgcgcgtag tgcaacgtcc ggcgcgtcgg ccggtcagtg cgcggaccgt
     9121 ctgcgctgca gctgggtcgc ggtcaacgag gagccgtcgt ggctccatcc cgggggcgcg
     9181 agcaggtagc cgagcttggc gccgaggccg ggcgcgcgga cgacgtcgcg gccgatcgcg
     9241 gcgaactcgt gaaacccgat ccgcagcggg ttgtaggtct ggatgtcctt ggtcaggccg
     9301 tacgccacgt gctcggcctc gggcgcgaac gtcccgaaca ggcgatccca gacgatgaag
     9361 atgcccccgt ggttcttgtc gaggtacggc acgttcttgc cgtggtgcac gcggtggtgg
     9421 gacggcgtgt tcatgaacca ctcgagcggc cccaggcgat cgaccgcctc ggtgtggatc
     9481 cagaactggt agaacagcga ccacgcctgg gcggtcagca ccatccacgg cggaaacccg
     9541 atcagcggca gcggcgccca gaagatcggg ccgctcagcg gggtgagcag cggctggcgc
     9601 agcgcggtcg acaggttgaa gcgctggctc gagtgatggt tgacgtggca cgcccacagc
     9661 aggcggacct cgtggtggac gcggtggaac cagtagtagc agaggtcctc gccgagcagc
     9721 agcacgatcc aggtccacca cgcggtgccg atgtcggcga cgcggtgctg gtagagccag
     9781 gcgaagaacg ggatcgagag cagcttggtg aaccccgaga cgacgacgtt gagcaggccc
     9841 atcgtcatcg acgccgcgga gtcgcgcagc tcgtagcccc ggatccggtc gtcgtcgcgg
     9901 tggcggcgcg cccacagggc ctcggccacg accgcgatca cgaacgccgg gatcgcgtag
     9961 agaatcaggt tcgacgcctg tgcggtgtcg gtggtggagg aggcaaggtt cgacacgtgg
    10021 cgacattgta tcccggtcgc gcccttccaa accccggttg gggcgcgcga tgctgcgcgc
    10081 catgacccat ggcggagaca tcatcgcgga cgtcctggcc cgccacggcg tgacccacct
    10141 gttcacgctg tgcggcggcc acatctcgcc gatcctgacc ggcgcacagg cgcgcgggct
    10201 gcgtgtcgtc gacgtgcgcg acgaggccaa cgcggtgttc gccgccgacg cggtcgcgcg
    10261 gatgaccggc acggtcggcg tcgcggcggt gacggcgggg ccgggggtga ccaacgcggt
    10321 caccgcggtc aagaacgcgc agatggcgca gtcgccggtg ctgctgctcg gcggtgcgac
    10381 cgcgaccctg ctcaagggcc gcggctcgct gcaggacatc gaccagatgg cggtgatgaa
    10441 gccgctgacc aagtgggcga cggcggtcaa gaccgtgccg gcgctggccc cggtgatcga
    10501 gcgcgcctgt cagatcgcga ccgagggcgt gccggggccg gtgttcgtcg aggtcccggt
    10561 cgacctcctg tatcccgagg cgacggtccg cgagtggtac gcccgggagg ccgggctcgg
    10621 caggggcaag gggatcggcg ccaaggcggt cgagctctac atccgcggcc acctgtaccg
    10681 ccagttccgc gcgccggcga tgcccgaggt ccacgtgccg gtcaagctgc cgagcctgtt
    10741 cagcgacggg ggggtcgagc gcgcggccga ggcgatcaag accgcgcagc ggccggtgtt
    10801 cgtgatcggc agccaggcgc tggtcggcgt caaggacccc ggccggctgg cgcgctcgct
    10861 cggcatgctc ggcgcgccgg tgttcctcgg cggcatggcg cgcggactgc tcggccggag
    10921 ccacgagctc cagctccgcc acaaccgcgg cgccgcgctc aagcgcgccg acgtgatcgt
    10981 cgtgtgcggc ttcccgttcg acttccggct cggctacggc cgcggcctca accgcgacgc
    11041 gaccctgatc gccgccaacc tgtcgccgca ggagctgcgc aagaaccgcc ggcccgacat
    11101 cgcggtcgag atgcacgccg gcgagttcgt gatcgcgctc gccgaccgga tcagctcggc
    11161 gggtgaccgc tggcggggct ggctcgacga gctgcgcgcc caggagcgcg cgcgcgacgc
    11221 cgagatcgcg gccagggccc agccggccgg cgagctggtc gatccgctcc acttcttcct
    11281 ccgcctcgag gaggcgatgg ccgacgacgc ggtgctggtg gtcgacggcg gcgacttcgt
    11341 ggcgaccgcg agctacatcg tccggccgcg cgcgccgctg tcgtggctcg acccgggcgc
    11401 gttcggcacg
//
LOCUS       DASVOR010000569         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238810,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000569 DASVOR010000000
VERSION     DASVOR010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238810"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VF516_11295"
     CDS             <1..317
                     /locus_tag="VF516_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108307.1"
                     /translation="IALDAHAAPCPRRIASEDAAHRILALVRRGAGACMPDALAPSIE
                     LAMPLPRPGAYHLAHVVLDGSFLRLYPDGMARAGVVFPVDDPGALHRIIAGLPAAAPI
                     RR"
     gene            complement(277..>1500)
                     /locus_tag="VF516_11300"
     CDS             complement(277..>1500)
                     /locus_tag="VF516_11300"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8108308.1"
                     /translation="LVARSRRQVRRQAAAVALLVAVAVAVPGYTAYRSHLASQRQERE
                     MALKARNLGQFVLSLEPFDWDPEAQRATAADPAPLGLDWQLQAWTDDGPGEPYVEHDD
                     VEHTAPRPDGAALVQDVEARGGRAYLTIRRRGCTPSLLPVKDLPGYAQRDRPIPTFHV
                     RVPTCAATAAGMIEIPAGLFRYGGAGTPPSRIAAELGPEQRIDLPRFWIDRTEVTNAA
                     FAMLADMAALTGIEAPSYPPTPILEHAADPKKPVTGVNWYTARDYCRYLGKDLPTSEQ
                     WVKAMRGGEQLADGSDNPIPDRNYPFGIGDPYRLAALIPSPGTADVATHPGDVSPYGV
                     LDLTGNAEEWTLTAYEGRGIRVLRGGGVAEQMGDALLDFMAIPNPRVASQPLFAIGER
                     CVVNDGSAPPPADRR"
BASE COUNT          179 a          535 c          579 g          207 t
ORIGIN      
        1 cgatcgcgct ggacgcgcat gccgcgccgt gtccgcgccg gatcgccagc gaggatgcgg
       61 cgcaccggat cctggcgctg gtgcggcgcg gcgcgggcgc atgcatgccg gatgcgctgg
      121 cgccgtcgat cgagctcgcg atgccgctgc cgcggcccgg ggcgtatcac ctcgcccacg
      181 tcgtgctgga cggcagcttc ctgcggctct accccgacgg gatggcccgg gcaggggtcg
      241 tgtttccggt cgacgacccg ggcgcgctgc accggatcat cgccggtctg ccggcggcgg
      301 cgccgatccg tcgttgacca cgcagcgctc gccgatcgcg aacagcggct gcgacgcgac
      361 ccgcgggttg gggatcgcca tgaagtcgag cagggcgtcg cccatctgct ccgcgacgcc
      421 gccgccgcgc agcacgcgga tgccgcgacc ctcgtaggcg gtgagcgtcc actcctcggc
      481 gttgccggtc aggtcgagca cgccgtacgg gctcacgtcc ccgggatggg tcgcgacgtc
      541 cgcggtcccc ggcgacggga tcagcgcggc gagccggtat ggatcgccga tcccgaacgg
      601 gtagttgcga tcggggatcg ggttgtccga accgtcggcg agctgctcgc cgccgcgcat
      661 cgccttgacc cactgctcgg aggtcggcag gtccttgccg aggtagcggc agtagtcgcg
      721 cgcggtgtac cagttgacgc cggtcaccgg ctttttcgga tcggccgcgt gctcgaggat
      781 cggcgtggga ggatagctcg gggcctcgat gccggtgagc gccgccatgt cggccagcat
      841 ggcaaacgcc gcgttggtca cctcggtgcg gtcgatccag aaccgcggca gatcgatccg
      901 ctgctccggc ccgagttcgg cggcgatccg tgacggcggg gtgccggcgc cgccgtagcg
      961 gaacagcccc gccgggatct cgatcatgcc ggcggcggtc gcggcgcagg tcggcacccg
     1021 gacgtggaac gtcgggatcg gccggtcgcg ctgcgcgtag cccggcaggt ccttgaccgg
     1081 caagagcgac ggcgtgcagc cgcggcgccg gatcgtgagg taggcgcgac cgccgcgcgc
     1141 ctcgacgtcc tggaccagcg cggcgccatc cggccgcggc gccgtgtgct cgacgtcgtc
     1201 gtgctcgacg tacggctccc ccggcccgtc gtcggtccac gcctggagct gccagtcgag
     1261 gccgagcggc gccgggtcgg cggcggtggc gcgctgggcc tcgggatccc agtcgaacgg
     1321 ctcgagcgac agcacgaact ggccgaggtt gcgcgccttg agcgccatct cgcgctcctg
     1381 gcgctgcgac gcgaggtggc tgcggtaggc ggtgtagccg gggaccgcga ccgcgaccgc
     1441 gaccagcagc gcgaccgccg cggcctggcg gcgcacctgc cggcgcgagc gcgcgaccag
//
LOCUS       DASVOR010000570         3974 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_63639,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000570 DASVOR010000000
VERSION     DASVOR010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3974
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63639"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="VF516_11305"
     CDS             complement(<1..770)
                     /locus_tag="VF516_11305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8108309.1"
                     /translation="MSVDAPELPFRDLIDAAPDGVIVCDPAGLIVLVNAETERMFGYR
                     RNELIGQRIDVLVPDRVRGQHNRHVLGYTGSPRLRPMGIGMELTGRRKDGGEIPVEIS
                     LSPIQTSRGLLVTAGIRDVTDRRKLERDAKRANAYLVSAVDSVRDAFALFDEHDRVIM
                     VNSAARQLFSAAGGGIIGLPFAELLDQSLRAGVFDFSNESREALYQRWMDYHRAPSGA
                     LEVRTGTGRYLRVTENRTAEHGTVSTIADVTDDEQRAE"
     gene            complement(1246..1818)
                     /locus_tag="VF516_11310"
     CDS             complement(1246..1818)
                     /locus_tag="VF516_11310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108310.1"
                     /translation="MLIVHRDLKPPLILSIALIASCLSTPESPEPEPEPELNTAAQAS
                     TAGASPEFAALAVAQLETDPAVRAMLMARAVARARFAVRGAQDVAINVPPELDLDDNY
                     TEGGHCNVRLTQCDPYPIGKGNHTEADAYCSRVCLDTIAHCEDYSIAEIEFCCAHPNK
                     DYKVNGKLIGHCMPSGEPNWYKRCAPGLNP"
     gene            2232..2807
                     /locus_tag="VF516_11315"
     CDS             2232..2807
                     /locus_tag="VF516_11315"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8108311.1"
                     /translation="MSTTKPSPRAPTLADLDALPPGVVGEIIEGVLYTMTKPRARHQR
                     TGLEIGSGLLGPFDHGRGGPGGWWIITEPGIQLPNTPEISPDVAGWRRERLPELPVDE
                     PIGVVPDWICEILSPTTRRHDQLVKLPYYARVGVPYAWVVDLESRVLNVYRLEEGTWR
                     IVGTYSDESEARIAPFEAVPLNVAAWWPPAG"
     gene            complement(3097..3169)
                     /locus_tag="VF516_11320"
     tRNA            complement(3097..3169)
                     /locus_tag="VF516_11320"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3134..3136),aa:Ala,seq:cgc)
     gene            3276..>3974
                     /locus_tag="VF516_11325"
     CDS             3276..>3974
                     /locus_tag="VF516_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEX8108312.1"
                     /translation="MHDSRACDLVVLAAMARERLDKLVADRGLAASRERARALIMTGD
                     VLVQGRPETKPGTLVDPAAEITLRAADHPYVSRGALKLVKGLDQFAVDPRGAVALDIG
                     ASTGGFTDVLLRRGAARVYAIDVGYGQLAWSIRQDPRVVVLERENARTIDPALVPEPC
                     DLAVIDVSFISLTLVLPRVRELLRPPPGKPIVALVTPQFEAGRAEADRGAGVISDPEV
                     HRRVLRELIGWLAAE"
BASE COUNT          541 a         1380 c         1455 g          598 t
ORIGIN      
        1 tcctcggcgc gctgctcgtc gtcggtgacg tcggcgatcg tcgacaccgt gccgtgctcg
       61 gcggtccggt tctcggtcac ccgcaggtag cggccggtgc cggtgcggac ctcgagcgcg
      121 cccgacggtg cgcggtggta gtccatccag cgctggtaca gcgcctcgcg gctctcgttg
      181 ctgaagtcga acacgccggc gcgcagcgac tggtcgagca gctcggcgaa cggcagcccg
      241 atgatgccgc cgccggcggc gctgaacagc tggcgcgccg cgctgttgac catgatcacg
      301 cggtcgtgct cgtcgaacag cgcgaacgcg tcgcgcaccg agtccaccgc cgataccagg
      361 taggcgttgg cgcgcttggc gtcgcgctcc agcttgcggc gatcggtgac gtcgcggatc
      421 ccggcggtga ccagcaggcc gcgcgacgtc tggatcggcg acaggctgat ctcgaccggg
      481 atctccccgc cgtccttgcg cctcccggtc agctccatgc cgatccccat cggccgcagc
      541 cgcggcgagc cggtatagcc gaggacgtgc cggttgtgct ggccgcgcac ccggtccggc
      601 accaggacgt cgatccgctg gccgatcagc tcgttgcgac gatagccgaa catccgctcg
      661 gtctcggcgt tgaccagcac gatcagcccc gccgggtcgc acacgatcac gccgtccggc
      721 gcagcatcga tgaggtcgcg gaacggcagc tccggtgcgt cgacggacat ggctcgtgct
      781 cagcctcccc ggtgcgcagg gcgaacgcaa gccggcggcc gtcggaaccg ccggacgccc
      841 ggagcccgac gcccgaacaa cgagagcccg aaccttcgcg attcgagctc ttgcgtcccg
      901 cggtggagcc aggggggatc gaacccctga cctcccgcgt gcgatgaaag accgtctgtg
      961 tgattccgag cagttacggc ccgatttgtt gcgacgcgcg ggtcgacgag ccgtgcagcg
     1021 tccgccggcg aaccagtggc gccaggaccg cgccaccgcg ccaccggatg accgccgggc
     1081 cggccggagc cggagcgcag gcgatggcga tggccagcgc gccgccgagg gcacaggcga
     1141 ccccgccgac gaatccgagc agggcgacga cggccctggt catggcgccg gaccgcggac
     1201 gcacctattc acctccactg gcacccccac tggcacccca ctggttcagg gattgaggcc
     1261 gggcgcgcac cgcttgtacc agttcggctc gccgctcggc atgcaatgcc ctatcagctt
     1321 cccgttgacc ttgtagtcct tgttggggtg cgcgcagcag aactcgatct ccgcgatcga
     1381 gtagtcctca cagtgggcga tcgtatcgag gcatacacgg ctgcagtacg cgtcggcttc
     1441 ggtgtggttg cctttgccga tcgggtacgg gtcgcactgc gtgagccgca cgttgcagtg
     1501 gccgccctcg gtgtagttgt cgtccaggtc gagctcgggc ggaacgttga tcgcgacgtc
     1561 ctgcgcgcca cgcactgcga acctggcccg cgccacggcc ctcgccatga gcatggcccg
     1621 cactgcggga tcggtctcga gctgcgccac ggccagcgcc gcgaactcgg gcgacgcgcc
     1681 cgcggtggac gcctgggcgg cggtgttgag ctcgggctcg ggctcgggct cgggcgactc
     1741 gggcgtcgag aggcacgaag caatcagggc gatggacagg atcaggggcg gcttgaggtc
     1801 gcggtggacg atcagcattt tcccgagata cggcgctcgc gcggtccgtg ggccgagcgt
     1861 cgggtcagcc gatcgtgatg gcgaggggcg tgctccgagg tcgcgtgcgc cctcgtcagt
     1921 ccgcgcgcgg ctagagtgcg atgaaagacc ggtcgttgga tttcgagcgg ttacggatcg
     1981 actcgttccg gtgcttgccg ttcgtcgtcg gtgcggcgtg cggaacggtc gtgcctgggc
     2041 tggtcccagg tgcgatcccg cgccaggcga tggtgcgggg tggacatcgc gctcctcgcc
     2101 gtggcgccgg gcgtggtctc gaggtcgact cggccgtgac cggcgtggct tggagtcgac
     2161 cctgcagatc gggcgcggcg atcggggacc cggcgaggac cgcgccacca gtgctaccct
     2221 cccggtgagc catgagcacg accaagccga gcccccgggc tccgacgctc gccgacctcg
     2281 acgccctgcc gccgggcgtc gtcggcgaga tcatcgaggg cgtgctctac acgatgacca
     2341 agccgcgggc tcgccaccag cgcactggcc tggagatcgg gagcggcctg ctgggcccgt
     2401 tcgaccacgg gcgcggcggc ccgggcggct ggtggatcat caccgagccc gggatccagc
     2461 tgcccaacac gcccgagatc tcgcccgacg tggccggctg gcgccgcgag cgcctgcccg
     2521 agctcccggt cgacgagccg atcggcgtgg tgcccgactg gatctgcgag atcctgtcgc
     2581 cgacgacgcg ccgccacgac cagctcgtca agctgccgta ctacgccaga gtcggcgtgc
     2641 cctacgcgtg ggtcgtagac ctcgagtccc gcgtgctgaa cgtctaccgg ctcgaggagg
     2701 gcacgtggag gatcgtcggc acgtacagcg acgagagcga ggcgcggatc gcgccgttcg
     2761 aggcggtacc gctgaacgtc gccgcctggt ggccgccggc ggggtgagca gcgcggaggg
     2821 cttccgccac actactacgc agagcctgtc gcgccccaag gcgacggggt gctcgctcgt
     2881 ccgacgcccg gcaggcccgg cggctcgagg gcggtcgagc ccggtgacgt caagtcgacg
     2941 gctgatctcc ggcgttggga accactcgat cttgttcagc tccagcttca ccttgcgcgt
     3001 gacctaccgt tccgtgaccg ttccgcagcc cggtcgcgcg tccggccgaa aacgccgaag
     3061 ggccccgcat cgctgcgaga ccctttggtt tcccggtgga gccaaggggg atcgaacccc
     3121 tgacctctag agtgcgattc tagcgctctc ccagctgagc tatgaccccg ggcaggcggt
     3181 atgggtatca gcgagggcgg ggtctgtcaa gccgcggtgg ccgtgtggat cgccgcggcg
     3241 cgggccgtgt tcgcgtgcgt attcaagggg ttgcaatgca cgactcccgg gcgtgcgatc
     3301 tggtagtcct ggcggcgatg gcgcgcgagc ggctcgacaa gctggtggct gaccgcggcc
     3361 tggcggcgag ccgggagcgg gcgcgcgcgc tcatcatgac cggcgacgtg ctggtgcagg
     3421 gccggcccga gaccaagccg gggacgctgg tcgacccagc ggccgagatc accctgcgcg
     3481 ccgcggacca cccgtacgtg tcgcgcggcg cgctcaagct ggtgaagggc ctcgaccagt
     3541 tcgcggtcga cccgcgcggc gcggtcgcgc tcgacatcgg cgcgtcgacc ggcgggttca
     3601 ccgacgtgct gctgcggcgc ggggcggcgc gggtctacgc gatcgacgtc ggctacggcc
     3661 agctcgcgtg gtcgatccgc caggacccgc gggtcgtggt gctggagcgc gagaacgcgc
     3721 ggacgatcga cccggcgctc gtgcccgagc cgtgcgacct cgcggtgatc gatgtgtcgt
     3781 tcatctcgct caccctggtg ctgccgcgcg tccgcgagct gctgcgcccg ccgcccggca
     3841 agccgatcgt cgcgctggtc acgccgcagt tcgaggcggg ccgcgccgag gccgaccggg
     3901 gtgccggcgt gatcagcgac ccggaggtcc accgccgcgt gctgcgcgag ctgatcggct
     3961 ggctggcggc ggag
//
LOCUS       DASVOR010000571         3377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_238844,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000571 DASVOR010000000
VERSION     DASVOR010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238844"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1517)
                     /locus_tag="VF516_11330"
     CDS             complement(<1..1517)
                     /locus_tag="VF516_11330"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8108313.1"
                     /translation="MRDDGNIAGRPPSLRSRLSRAPLNQAELILRDVVQARLAELLGW
                     PEVRQLAPDQSLLELGIDSLRAVDFKLLLEAELDCSLRSSLLFDHPTMAALVRYLAAD
                     ALGLAPAEPEIVHASSEQLQAIARARAEPIAVVGIGCRFPGGQEVASTAEQFWQLQLD
                     AVDAITEVPPTRWDASAFYDPDPAAPGKMATRHGGFLGDLTRFDAQFFGISAREATQM
                     DPQQRLLLECAWEAIEHAGQAPRQLSGAPVGVFVGCRSSEYGWSRHGLSTEGVGPYFN
                     VGDSTAMTSGRLSYLLGLTGPSVALDTACSSSLVAVHLACEAIRRGECSAALAGGVHV
                     MVMATTSVGLSKAGMLSRDGRCKTFSAAANGYVRAEGCGVVYLKRLSDARRDGDRVLA
                     VVRGSAANQDGASAGLTVPSGAAQEAVIRRALQVAGIAPGDVGYIEAHGTGTSLGDPI
                     ELNALGAVFAAGRDRARPLVIGSVKTNIGHAEPAAGIAGLIKCVMALRHGAIPAHL"
     gene            complement(1611..2729)
                     /locus_tag="VF516_11335"
     CDS             complement(1611..2729)
                     /locus_tag="VF516_11335"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8108314.1"
                     /translation="MTPIDQLVTRSLIDIPSWEVHASAGCVILDNNESPYPLDPDLAA
                     ELGAELAAVCLHRYPDPEASGLRELIARLHGVPADQVAVMNGMNDALFTLVGLFAEPR
                     AGETARVMYVVPSYVAYRMIALRLRLEPVEVPLADDFALDEPAIERALAIRRPNVVVL
                     ARPNNPTGTLWPREVVAGLVERNPDVVFISDEAYVEFAGDDASLIDLVPRLPNLVVTR
                     TLSKAYGMAGLRLGWAVASPAVMRQFAKARFIYNVGALNQAAAAWLLSRHRDRILARV
                     RTIVAERERIAGELGQLPGVRVVPSHGNFLLLRCGRPGDGRAAALWQAVYRRGVLVRD
                     FDRPGPLSGCLRVTVGTPEDNARLLDAMTAAVAALGSA"
     gene            complement(2778..>3377)
                     /locus_tag="VF516_11340"
     CDS             complement(2778..>3377)
                     /locus_tag="VF516_11340"
                     /inference="COORDINATES: protein motif:HMM:NF017819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HEX8108315.1"
                     /translation="VAPPAVPATVPPASEVLEFWFGEPARSLWFTTRPAFDDEIRSRF
                     GAVAAAAAAGELDPWAASSDGALALTITLDQFPRNIHRGTPAAFAHDGLARAVAGRAI
                     ERGFDLAVSLDRRMFFYLPFEHSEALADQERSVELFSRWVSEHPDERRAHAGDQMTYA
                     VRHHEIIRRFGRFPHRNAILGRAPTPEEIEFLCEPRSSF"
BASE COUNT          504 a         1249 c         1196 g          428 t
ORIGIN      
        1 aggtgggccg ggatcgcgcc gtgccggagc gccatcacgc acttgatgag gccggcgatc
       61 ccggcggcgg gctcggcgtg gccgatgttc gtcttcaccg agccgatcac cagcgggcgc
      121 gcccgatcgc ggccggccgc gaacaccgcg ccgagcgcgt tgagctcgat cggatcgccc
      181 agcgacgtcc cggtgccgtg cgcctcgatg tagccgacgt cgccgggcgc gatccccgcg
      241 acctgcagcg cgcggcggat caccgcctcc tgggccgctc cgctgggcac ggtcaggcca
      301 gcgctggcgc cgtcctggtt cgctgccgag ccgcgcacca ccgcgagcac gcgatcaccg
      361 tcgcggcgcg cgtccgacag ccgcttgagg tacaccacgc cgcagccctc cgcgcgcacg
      421 tagccgttcg cggcggcgga gaacgtcttg cagcgcccgt cgcgcgatag catgcccgcc
      481 ttcgacagcc cgacgctggt cgtcgccatc accatcacgt ggacgccgcc ggcgagcgcc
      541 gcgctgcact cgccgcggcg gatcgcctcg cacgcgaggt ggaccgcgac cagcgacgag
      601 gagcacgccg tgtcgagcgc cacgctcggc ccggtcaggc caagcaggta cgacagccgg
      661 cccgacgtca tcgcggtcga atcgccgacg ttgaagtacg gtccgacacc ctcggtcgac
      721 aggccgtgcc gcgaccagcc gtactccgac gagcggcagc cgacgaacac ccccaccggc
      781 gccccggaca gctgccgggg cgcctgcccg gcgtgctcga tcgcctccca cgcgcactcc
      841 agcagcagcc gctgctgggg atccatctgg gtcgcctcgc gcgccgagat cccgaagaac
      901 tgggcatcga accgggtgag gtcgccgagg aagccgccgt ggcgggtcgc catcttcccc
      961 ggcgccgccg gatcgggatc gtagaacgcc gaggcgtccc atcgggtggg cggcacctcg
     1021 gtgatcgcat cgacagcgtc cagctgcagc tgccagaact gctccgcggt cgacgcgacc
     1081 tcctggccgc cggggaaccg gcagccgatg ccgacgacgg cgatcggctc ggcgcgcgcc
     1141 cgcgcgatcg cctggagctg ctccgacgac gcgtgaacga tctccggctc cgcgggcgcc
     1201 aggccgagcg cgtcggcggc gaggtagcgc acgagcgccg ccatcgtcgg gtgatcgaag
     1261 agcagcgagc tgcgcagcga gcaatcgagc tcggcctcca gcaggagctt gaagtcgacg
     1321 gcgcgcagcg aatcgatgcc gagctccagc aggctctggt ccggggccag ctgccggacc
     1381 tccggccatc ccaggagctc ggcgagccgc gcctgcacga cgtcgcggag aatcagctct
     1441 gcctggttca gcggtgcacg cgacagccgg gaccggagcg acggtggccg tcccgcgatg
     1501 ttcccgtcgt cgcgcacagc acctcctgga gcccagaaat gctacttgcg acgcgtggca
     1561 acggtcaagg atcgccgccc gaaagccgcg gcgccgcttc ggtgaccgtg tcaggcgctg
     1621 ccgagcgcgg ccacggcggc ggtcatggcg tcgagcagcc gcgcgttgtc ctccggcgtg
     1681 ccgacggtga cgcgcaggca gccggacagc ggacctgggc gatcgaagtc tcgtaccaga
     1741 accccgcgcc ggtacaccgc ctgccacagc gcggctgccc gaccgtcgcc cggccggccg
     1801 cagcggagca gcaggaagtt gccgtgggac ggcaccacgc gcacgccggg cagctgcccg
     1861 agctcacccg cgatccgttc gcgctcggcc acgatcgtgc gtacgcgggc gaggatccgg
     1921 tcgcgatgcc gcgatagcag ccacgccgcc gccgcctggt tcagcgcacc gacgttgtag
     1981 atgaaccgcg ccttggcgaa ctgccgcatg accgccggcg acgcgaccgc ccagcccagc
     2041 cgcagcccgg ccatgccgta ggccttcgac agcgtgcggg tcaccaccag gttggggagc
     2101 cgcggcacga ggtcgatcag cgacgcatcg tcgcccgcga actcgacgta ggcctcgtcg
     2161 gagatgaaca cgacgtcggg gttgcgctcg acgagccccg ccacgacctc gcgcggccat
     2221 aacgtcccgg tcgggttgtt cgggcgggcg agcacgacga cattgggccg acggatcgcc
     2281 agcgcccgct cgatcgccgg ctcgtcgagg gcgaagtcgt cggcgagcgg gacctccacc
     2341 ggctccagcc ggagccgcag cgcgatcatg cgatatgcga cgtacgacgg cacgacgtac
     2401 atcacgcgcg cagtctcgcc ggcccgcggc tccgcaaaca gcccgaccag cgtgaacagc
     2461 gcgtcgttca tcccgttcat cacggcgacc tggtcggcgg gcacgccgtg cagccgcgcg
     2521 atcagctcgc gcagccccga cgcttcggga tcggggtatc gatgcaggca caccgcggcg
     2581 agctctgccc cgagctcggc ggcgaggtcg gggtcgagcg gatacgggct ctcgttgttg
     2641 tcgaggatca cgcagcccgc ggacgcgtgg acctcccagc tcgggatgtc gatcagcgac
     2701 cgcgtcacca gctggtcaat cggtgtcatc cgaacctgaa cggccatcgt ctgcggctcc
     2761 ttgctccgca cgttatctca gaacgacgat ctcggctcgc acaggaactc gatctcctcg
     2821 ggagtcggcg cgcgaccgag gatcgcgttg cggtgcggga accggccgaa ccgccggatg
     2881 atctcgtggt ggcggaccgc gtacgtcatc tgatcgccgg cgtgggcccg gcgttcgtcc
     2941 gggtgctcgc tgacccagcg cgagaacagc tcgaccgagc gctcctggtc cgcgagcgcc
     3001 tcgctgtgct cgaacggcag gtagaagaac atccggcgat cgagcgacac ggccaggtcg
     3061 aacccgcgct cgatcgcgcg gccggcgacc gcgcgggcca ggccgtcgtg cgcgaacgcc
     3121 gccggtgtgc cgcggtggat gttgcggggg aactggtcga gggtgatggt cagcgcgagc
     3181 gcgccgtcgc tgctcgccgc ccaggggtcg agctcgccgg ccgcggcggc cgcggccacc
     3241 gcgccgaacc gcgaccggat ctcgtcgtcg aacgccggcc gggtggtgaa ccacagcgat
     3301 cgcgccggct cgccgaacca gaactccagg acctcgctcg ccggcggcac cgtcgctggg
     3361 actgctgggg gggctac
//
LOCUS       DASVOR010000572         3729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_326761,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000572 DASVOR010000000
VERSION     DASVOR010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326761"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VF516_11345"
     CDS             <1..1229
                     /locus_tag="VF516_11345"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HEX8108316.1"
                     /translation="RGDGGWDEDAYTGCGFPGYGIGEPRGARIRQGREMASAFLIRYH
                     LYRNCFPLLALGRYRTAINRAGRTPARSSASYQGRLDAVSRSFAMCIPQLDLPLRDRV
                     ALSYLLMRVLDTVEDAPFTDKAEQARQFQRLRGFLRAPPRRAEVEAFVAGFPAELPDG
                     ERALLGDTAALLDDAHALPAAVRAAMFSAVDRMAIGMAAYARRPSGLRLVDLEDVTRY
                     CCFVAGVVGELLTALFAIGKTPPAPPMVLAYHFGLFLQKVNILKDQQEDEAAGRFLVP
                     DRRALLASLRHDAQGALEYLQALPRGERGYRTFCAWSLMMGAVTVANLDQPRRSRRDE
                     TAELLARTAAIAQDNAALGRLFAELLPELPVVAPGPAQDKPESVEWFRAVLAAPLSDA
                     ELRRLGIAVRASALAG"
     gene            1530..2546
                     /locus_tag="VF516_11350"
     CDS             1530..2546
                     /locus_tag="VF516_11350"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF023569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HEX8108317.1"
                     /translation="MPLAVCHASHWILDAAIAELKAAVAAIDRAALRNGGEVPADQAS
                     EFQNRFAAFCLLLNEHLGDSSPRDEETYEALGAHLQRELLPYMLLTENCERWYAKPRG
                     YAGDFLSIEWIYRNRAGGSGRIGPLLDRCFLDLPAATAVRNRRGLLREEIEKTIAARQ
                     SATARVLSLASGPARELFDVYEALEDPAILAATCVDIDLQALAFVADLRDARKLKRTM
                     HLVNANLVHVATSRAKLELPPQDLVYSIGLIDYFEDRFVVRLLDAIHDRLRPGGRVIL
                     GNFHPDNPNRAVMDHLLDWKLIHRTEDDMNRLFQASKFGRPCDEIRFEAERINLFAIG
                     SRAP"
     gene            2629..3612
                     /locus_tag="VF516_11355"
     CDS             2629..3612
                     /locus_tag="VF516_11355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8108318.1"
                     /translation="MRAVPVVLVTAACAHAQVVPPRPAADFVRDTHAMLDAFDRGDAA
                     AVAAITAPNFVRFEAGKVHERAAELARLKPRPPEITRAWKDEHVYIGVHDATFIGLAA
                     EHEIGNDSHGNRETDGWYVVAWTRGGATWKVAHWTWQPYRSTLDLQRDVWNDNFRQDI
                     GFSHEPNQLLVTTMAGVTPGAALDLATGQGRNALYLASHGWTVTAVDISDEGLRRARE
                     AAAHSHVALDTVQADIDAYDYGVAMWDLVTEIYVPNPVARAGKIKAALRSGGLFVLEF
                     FLDEGDGGVKPAELRAQFADGFDILRDEVIEDRPDWGLDRAKLVRFVARKR"
BASE COUNT          534 a         1348 c         1291 g          556 t
ORIGIN      
        1 agcgcggcga cggcggctgg gacgaggacg cctacaccgg ctgcgggttc ccgggctacg
       61 ggatcggcga gccgcgcggc gcgcggatcc ggcagggccg cgagatggcg tcggcgttcc
      121 tgatccggta ccacctgtac cgcaactgct tcccgctgct cgcgctcggc cgctaccgca
      181 cggcgatcaa ccgggccggg cgcacgccgg cgcggtcgtc ggcgtcctac cagggccggc
      241 tcgacgcggt gagccgcagc ttcgcgatgt gcatcccgca gctcgacctg ccgctgcgcg
      301 atcgcgtggc gctgtcctac ctgctgatgc gcgtgctcga caccgtcgag gatgcaccgt
      361 tcaccgacaa ggccgagcag gcgcggcagt tccagcgcct gcgcggcttc ttgcgcgcgc
      421 cgccgcggcg cgccgaggtc gaggcgttcg tcgcggggtt ccccgccgag ctgcccgacg
      481 gcgagcgtgc gctgctcggc gacaccgccg cgctgctcga cgacgcccac gcgctgccgg
      541 ccgccgtgcg cgccgcgatg ttcagcgcgg tcgatcgcat ggcgatcggc atggcggcct
      601 acgcgcggcg gccgtccggg ctgcggctgg tcgacctcga ggacgtgacc cgctactgct
      661 gcttcgtcgc cggcgtggtc ggcgagctgt tgaccgcgct gttcgcgatc ggcaagaccc
      721 cgccggcgcc gccgatggtg ctcgcgtacc acttcgggct gttcctccag aaggtcaaca
      781 tcctcaagga ccagcaggag gacgaggccg ccgggcggtt cctggtcccc gaccggcggg
      841 cgctgctcgc gagcctgcgc cacgacgcgc agggcgcgct cgagtacctg caggcgctgc
      901 cgcgcggcga gcgtggctac cggacgttct gcgcgtggtc gctgatgatg ggcgcggtca
      961 cggtggcgaa cctcgaccag ccgcggcgga gccggcgcga cgagaccgcc gagctcctgg
     1021 cgcgcaccgc cgcgatcgct caggacaacg ccgcgctcgg ccggctgttt gccgagctcc
     1081 tgcccgagct gccggtcgtc gcgcccggcc cggcgcagga caagccggag tcggtcgagt
     1141 ggtttcgcgc cgtgctcgcg gcgccgctgt ccgacgccga gctgcgccgg ctcggcatcg
     1201 cggtccgggc atcggcgctg gcggggtgac gactctttgc cgatgggcgt ctcggccccc
     1261 gacgccacaa tgacacgaac ctgatacgga ttctcgctca caatggcacg aacctgatac
     1321 gggtctctcc cggtgccgct ggtcagtcga tgcgccggtc gggtgtgcca gcgcgagaac
     1381 cgcattcgag acgcgagagg gcgtgtgatc ccccggcggt ccagcgatga gccccggtcg
     1441 gcgcgagcac ggcacgccgg ttgctgagtg cggcgcggat gcttgaaacc acgatctcgt
     1501 tcgagtcgac gaggcaacgt tcttccggga tgcctctcgc ggtctgtcac gccagtcact
     1561 ggatcctcga cgcggcaatc gccgagctga aggcggcagt cgccgccatc gatcgagcgg
     1621 cgctgcgcaa tggcggcgag gttccggccg accaggcgag cgagttccag aaccggttcg
     1681 ccgcattttg cttgctgctg aacgagcacc tcggcgactc gtcaccacgc gacgaggaaa
     1741 cctacgaggc gctgggcgcc catctccagc gcgagctcct gccgtacatg ctgctgaccg
     1801 agaactgcga gcgctggtac gcgaagccgc gcggctatgc gggggacttc ctgtcgatcg
     1861 agtggatcta ccggaaccgg gcagggggca gcggccggat cggcccgctg ctcgaccgat
     1921 gcttcctcga cctcccggcg gcgacggcgg tgcgcaatcg ccggggcctc ttgcgcgagg
     1981 agatcgagaa gacgatcgcc gcgcgccaga gcgcgaccgc gcgcgttctc agcctggcgt
     2041 ccggccccgc gcgcgagctg ttcgacgtct acgaggcgct cgaggatccg gcgatcctcg
     2101 cggccacgtg cgtcgacatc gatctgcagg cgctcgcgtt cgtcgccgac ctgcgcgacg
     2161 ccaggaagct caagcgcacc atgcacctcg tgaacgcaaa cctcgtccac gtcgcgacga
     2221 gccgcgcgaa gctcgagctt ccgccgcagg atctcgtgta cagcatcggc ctcatcgact
     2281 acttcgagga tcggttcgtg gtccgcctgc tcgacgcgat ccacgatcgc ctccggcccg
     2341 ggggtcgggt catcctgggc aacttccacc cggacaaccc gaatcgcgcg gtcatggatc
     2401 acctgctcga ctggaagctg atccaccgca ccgaggacga catgaaccgg ctgttccagg
     2461 cctcgaagtt cggccggccg tgtgacgaga tccgcttcga ggccgagcgc atcaacctgt
     2521 tcgcgattgg ctcgcgcgcg ccgtgaccgc gcctgctgtc cgtgttcctc gacaggcctc
     2581 cgcgagctcg tacgggaatg ccagccgcgc gcgcccggta gatctcgcat gcgcgccgtc
     2641 ccggtcgtcc tcgtcacagc tgcctgcgca cacgcgcagg tggtcccccc tcgcccggcc
     2701 gccgacttcg tgcgcgatac ccacgcgatg ctcgacgcgt tcgatcgcgg cgacgccgct
     2761 gcagtcgctg ccatcacggc gccgaacttc gtgcggttcg aggccggcaa ggtccacgag
     2821 cgcgcggcag agctcgcgcg gctcaagccg cggccgccgg agatcacgcg cgcctggaag
     2881 gacgagcacg tctacatcgg cgtgcatgac gcgacgttca tcgggctcgc ggccgagcac
     2941 gagatcggca acgattcgca cggcaaccgc gagaccgatg gctggtacgt ggtggcgtgg
     3001 acgcgcggcg gtgccacctg gaaggtcgcg cactggacct ggcagccgta ccgcagcacg
     3061 ctcgaccttc agcgcgacgt ctggaacgac aacttccgcc aggacatcgg cttctcgcac
     3121 gagccgaacc agctgctcgt gacgacgatg gccggcgtca caccgggcgc ggcgctcgac
     3181 ctcgcgaccg gccagggccg caacgcgctc tacctcgcga gccacggctg gacggtcacc
     3241 gcggtcgaca tctcggacga agggctgcgc cgcgcccgcg aggcggccgc ccactcgcac
     3301 gtcgcgctcg acaccgtgca agcagacatc gacgcctacg actacggcgt cgcgatgtgg
     3361 gacctcgtca ccgagatcta cgtcccgaac ccggtcgcac gcgccggcaa gatcaaggcc
     3421 gccctgcggt ccggcggcct gttcgtgctc gagttcttcc tcgacgaggg cgacggcggc
     3481 gtcaagcccg ccgagctgcg agcgcagttc gccgacggtt tcgacatctt gcgcgacgag
     3541 gtcatcgagg accgccccga ctggggcctg gatcgcgcga agctcgtgcg gtttgtcgcg
     3601 cgcaagcgct gacgtcactt ccgcgcagct tcagccgacg taggtgatcg accactcgcg
     3661 gacgtcgccg tcgaccgtga tgtcgacgac gtcgccgctc ttcttgccga gcacggcgcg
     3721 gccgatcgg
//
LOCUS       DASVOR010000573         1747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_34365,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000573 DASVOR010000000
VERSION     DASVOR010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34365"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..681)
                     /locus_tag="VF516_11360"
     CDS             complement(<1..681)
                     /locus_tag="VF516_11360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108319.1"
                     /translation="MMMVATTAAPPGGGAPSDLVVMDPGGGHRVQVTHDDALEFLPHF
                     APDGTRLLYTKFETGTYGTPGAITDIASYDFTTGQETLLTTRGDAAQAAWSPDGSTIA
                     FLTHSASMQSAIWLMDRDGGHPRQLAAASGADSDAVWGDIAWSSDDWILFVVAEQPAG
                     PCFKTRLDKMRPDGSARTKVSDGGASCTPAGMEQSGDADPGFSACGATIYTSRGLPHA
                     PAGAAAPRT"
     gene            complement(859..>1747)
                     /locus_tag="VF516_11365"
     CDS             complement(859..>1747)
                     /locus_tag="VF516_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8108320.1"
                     /translation="GEILKSLAVNVIIAASKGVAAFITGSGAMLAETLHSFADCGNQL
                     LLLQGVRATRRPPDRAHPFGYGRAMYFYSFIVALLLFFGGGVFSIYEGVHKLEHPEPV
                     QDTGVALIILLGSIALEGWSTLGNIRAMNQRRGSLGFYRYLRATKDSDLIVVFGENSA
                     AVLGLTFAIAALGLAHETGDARWDAIGSIAIGGVLVLVAAFLAREVKSLLVGEAADPA
                     LIRSFEELAELDPNVDRVLNVLTLQQGPGEIIVAAKLQFRPGMDTDTLVEAINAFERA
                     LKAKVPEVRWSFIEPDNTD"
BASE COUNT          254 a          597 c          655 g          241 t
ORIGIN      
        1 ggtgcggggc gcggccgcgc ccgccggcgc gtggggcagg ccgcgcgacg tgtagatcgt
       61 cgcgccgcag gcggagaacc cggggtcggc gtcgccgctc tgctccatgc cggcgggcgt
      121 gcagctcgcg ccgccgtcgc tgaccttggt gcgcgcgctg ccgtcgggcc gcatcttgtc
      181 gagccgggtc ttgaagcacg gtccggccgg ctgctcggcg acgacgaaca ggatccagtc
      241 gtcgctcgac cacgcgatgt cgccccacac ggcgtcgctg tcggcgccgc tcgccgccgc
      301 gagctggcgc gggtggccgc catcgcgatc catgagccag atcgccgact gcatcgacgc
      361 ggaatgggtg aggaacgcga tcgtcgagcc gtcgggcgac cacgcggcct gcgccgcgtc
      421 gccgcgggtg gtgaggagcg tctcctgccc ggtcgtgaag tcgtagctcg cgatgtcggt
      481 gatcgcgccg ggcgtgccgt aggtcccggt ctcgaacttc gtgtagagca ggcgcgtgcc
      541 gtcgggcgcg aagtgcggca ggaactccag cgcgtcgtcg tgcgtgacct ggacgcggtg
      601 gccgccgccc gggtccatga cgaccagatc cgagggcgcg ccgccgcccg gaggcgccgc
      661 ggtggtggcg accatcatca cgatggcagc atcggagacc acccgggggt ctgtcgagca
      721 cgccgcgacg atccagagcg caatcaggac gcgagccatg ccctgagtga ctccagccgg
      781 cccgcgatcg gacacgcggg gcggcaagac gttccgccgg gttcgccgag gtcgcggtcc
      841 atgacgcagg ccgtcagctc agtcggtgtt gtcgggctcg atgaagctcc accggacctc
      901 ggggaccttg gccttgagcg cgcgctcgaa cgcgttgatc gcctcgacca gcgtgtcggt
      961 gtccatgccg gggcggaact ggagcttggc cgcgacgatg atctcgcccg gcccctgctg
     1021 cagggtcagc acgttgagca cgcggtcgac gttggggtcg agctcggcga gctcctcgaa
     1081 cgaccggatc agcgcgggat cggcggcctc gccgaccagc aggctcttga cctcgcgcgc
     1141 caggaacgcc gcgaccagca ccagcacgcc gccgatcgcg atcgagccga tcgcgtccca
     1201 ccgggcgtcg ccggtctcgt gggccaggcc gagcgcggcg atcgcgaagg tcaggccgag
     1261 caccgcggcg ctgttctcgc cgaacacgac gatcaggtcg gagtccttgg tcgcgcgcag
     1321 gtagcggtag aacccgaggc tgccgcgccg ctggttcatg gcgcggatgt tgccgagcgt
     1381 cgaccagccc tcgagcgcga tcgaacccag gaggatgatc agcgcgaccc cggtgtcctg
     1441 gaccggctcg gggtgctcga gcttgtgcac gccctcgtag atcgagaaca cgccgccgcc
     1501 gaagaacagc agcagcgcga cgatgaacga gtagaagtac atcgcgcggc cgtagccgaa
     1561 cgggtgggcg cgatccggcg gccggcgggt cgcgcgcacg ccctgcagca gcagcagctg
     1621 gttgccgcag tcggcgaacg agtgcagggt ctcggcgagc atcgcgcccg agccggtgat
     1681 gaacgcggcg acgcccttgg acgccgcgat gatgacgttg acggcgagcg acttgaggat
     1741 ctcgccg
//
LOCUS       DASVOR010000574         2394 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_34382,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000574 DASVOR010000000
VERSION     DASVOR010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2394
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34382"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..578
                     /locus_tag="VF516_11370"
     CDS             <1..578
                     /locus_tag="VF516_11370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108321.1"
                     /translation="APASAAPSPAARALARPVRTAAPRAASVVEALDLKVRYDDPSNF
                     ASSRADAEREAGGLDEGREIGAESRRQLEDSVAHLEIPMPPAPSFARAPRAKHDYHRL
                     RLHSVRRFAGLTIKLLLTIDAQGRVSDIKLIQGIDGQLDLRVVQLARRFEFEPGLDQD
                     GTPIPGISRWDIQVVDDDNGQLRNAFERGYF"
     gene            complement(597..>2394)
                     /locus_tag="VF516_11375"
     CDS             complement(597..>2394)
                     /locus_tag="VF516_11375"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8108322.1"
                     /translation="ADGLNYYLATHPAVKPRVIARFEPWMALSFTEGSIGGDIERIDL
                     GELAAFYGENSTRPAPADTSELVKEPSGSNGFAIAPARTADHHALLLINPHTSFYFRS
                     ELQVTSDEGLHAYGAVTWGQFFVYQGFNDRVGWMHTSTGADAVDEYAESVVQRGDRLY
                     YRHGKALRPLEVGRIEVPYRTATGMASKSITVYRTHHGPIVRSADGKWIAIRLMQKPI
                     EALSQSYLRTKARDLAGFRKVMELAANSSNNTIYADADGHIAYFHPQFVPRRDDRFDW
                     TRPVDGSDPATEWNGVHGVDDSPHVIDPPNGWVMNTNNWPYSAAGPNSPRRDRFPRYM
                     DMHGENPRGLHATRVLDASRRFTLAGLRDAAFDSYLPAFAELIPPLVRAYDDAPSSDP
                     LKDKLGDPIRALREWNFRWSASSVPTSLAVYWGQAQWREAEGDARRTGGSVYDAIAAR
                     TPAQKLAALATAVDRLTTDFGSWRTPWGEINRFQRLTGDVVPHFDDEAPSIPVPFTSA
                     QWGSLASFGARTYPGTRRMYGTSGNSFVAVVEFGDRIKAMAVTAGGESGHPDSPHFLD
                     EAPRYASGDLRELYFYPEQLAGHTERTYHPGQ"
BASE COUNT          320 a          862 c          853 g          359 t
ORIGIN      
        1 cggccccggc gtccgccgcg ccgtccccgg ccgctcgggc gctcgcgcgt ccggtccgca
       61 ctgcggcgcc gcgggcggcc tccgtggtcg aggcgctcga cctgaaggtc cgctacgacg
      121 acccgagcaa cttcgcgtcc agccgcgccg acgccgaacg cgaggcgggc ggcctcgacg
      181 aggggcgcga gatcggcgcc gagagccgcc gccagctcga ggacagcgtg gcccacctgg
      241 agatccccat gcctccggca ccgtcgttcg cgcgcgcgcc gcgcgccaag cacgactacc
      301 accggctgcg gctccactcg gtgcgcaggt tcgccgggct gaccatcaag ctcctgctga
      361 cgatcgacgc gcaaggccgg gtcagcgata tcaagctgat ccagggcatc gacggccagc
      421 tcgatctccg tgtcgtccag ctcgcgcgcc gcttcgagtt cgagcccggg ctcgaccagg
      481 acggcacgcc gatccccggg atctccaggt gggacatcca ggtcgtcgac gacgacaacg
      541 gtcagctgcg caacgcgttc gagcgcggct acttttgatc ccggcggatc tcggcgctac
      601 tgcccggggt ggtacgtccg ctcggtgtgg ccggcgagct gctcgggata gaagtacagc
      661 tcgcgcaggt cgccgctcgc gtagcgcggg gcctcgtcga ggaagtgcgg cgagtccggg
      721 tggccgctct cgccgccggc ggtcaccgcc atcgccttga tccggtcgcc gaactcgacc
      781 accgcgacga agctgttgcc gctcgtgccg tacatccgcc tggtccccgg gtaggtccgc
      841 gcgccgaacg acgccaagga cccccactgc gcgctggtga acggcaccgg gatgctcggc
      901 gcctcatcgt cgaagtgcgg gacgacgtcg ccggtcagcc gctggaaccg gttgatctcg
      961 ccccacggcg tgcgccacga tccgaagtcg gtggtgagcc ggtcgaccgc ggtggcgagc
     1021 gcggcgagct tctgggccgg cgtgcgcgcg gcgatcgcat cgtagaccga gccgccggtg
     1081 cgcctcgcgt cgccttccgc ctcgcgccac tgcgcctgcc cccagtacac cgcgagcgac
     1141 gtcggcaccg agctcgccga ccaccggaag ttccactcgc gcagcgcgcg gatcggatcg
     1201 ccgagcttgt ccttgagcgg atcggacgac ggcgcgtcgt cgtaggcgcg gaccagcggc
     1261 gggatcagct cggcgaacgc cggcaggtag ctgtcgaacg cggcgtcgcg cagcccggcc
     1321 agcgtgaacc gccggctggc gtcgagcacg cgcgtcgcgt gcaggccgcg cgggttctcg
     1381 ccgtgcatgt ccatgtagcg cgggaagcga tcgcggcgcg ggctgttcgg ccccgccgcc
     1441 gagtacggcc agttgttggt gttcatcacc cagccgttgg gcgggtcgat cacgtgcggg
     1501 ctgtcgtcga cgccgtgcac gccgttccac tcggtcgccg gatcgctgcc gtcgaccggc
     1561 cgggtccagt cgaagcggtc gtcgcgccgc ggcacgaact gggggtggaa gtacgcgatg
     1621 tggccgtcgg cgtcggcgta gatcgtgttg ttcgacgagt tggcggcgag ctccatcacc
     1681 ttgcggaacc cggcgaggtc gcgcgccttg gtccgcaggt acgactggct cagcgcctcg
     1741 atcggcttct gcatcagccg gatcgcgatc cacttgccgt ccgccgagcg gacgatcggc
     1801 ccgtggtggg tgcggtagac cgtgatcgac ttgctcgcca tcccggtcgc cgtccggtac
     1861 gggacctcga tccggccgac ctcgagcggc cgcagcgcct tgccgtggcg gtagtacagc
     1921 cggtcgccgc gctggaccac cgactcggcg tactcgtcga ccgcgtcggc gccggtcgac
     1981 gtgtgcatcc agccgacccg gtcgttgaag ccctggtaga cgaagaactg gccccaggtc
     2041 accgcgccgt aggcatgcag cccctcgtcg ctggtcacct gcagctcgga gcggaagtag
     2101 aacgacgtgt gcgggttgat cagcagcagc gcgtggtgat cggccgtgcg cgccggcgcg
     2161 atcgcgaagc cgttcgagcc cgacggctcc ttcaccagct cgctggtgtc ggccggcgcg
     2221 gggcgcgtgg aattctcgcc gtagaacgcc gcgagctcgc cgaggtcgat gcgctcgatg
     2281 tcgccgccga tgctgccctc ggtgaacgac agcgccatcc acggctcgaa ccgcgcgatc
     2341 acccgcggct tcaccgccgg atgggtcgcc aggtagtagt tgagcccgtc ggcc
//
LOCUS       DASVOR010000575         5804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_502194,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000575 DASVOR010000000
VERSION     DASVOR010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5804
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502194"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(305..3154)
                     /locus_tag="VF516_11380"
     CDS             complement(305..3154)
                     /locus_tag="VF516_11380"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8108323.1"
                     /translation="MRASSRFTVLICSVVTAGLVAADPPPVHGQPRGGAITVDIPFEQ
                     FVLPNGLRVLVHTDRKAPIIAVNIWYHVGSKNEAKGRSGFAHLFEHLMFQGSENFKGE
                     FFEPFELVGATDQNGTTNGDRTNYFENVPTTAVDVALWMESDRMGHLIGAIDQHLLDE
                     QRGVVQNEKRQGENQPYGRAFTMILKAIYPEDHPYHHSTIGSMNDLDAAKLDDVKNWF
                     RSWYGPNNAVLVLAGDIDLATAKEKVTRYFGDIAPSANLARLSPKPALRTTATRETAT
                     DNVPQTRIYKVWNGPQFGSADSDRLLLLGQVLGGSKSSRLDKRLTFTEKLADSVGAFV
                     QLQEIGGAVIAQADVKAGVDPAKVEAAIDEEIGKLITEGPTAAEVEQARAAVLAGFIR
                     RVERIGGFGGKADVLAQCQVFTGNPACYRTTLANLQSATAAQLKEVAGKYLARGAFTL
                     QIDPGERKPVVEEPAVPNLPPTQVAPPARGLKAVPQTVDRSIGVPKTDSFPSLRFPAV
                     ARATLSNGVQVVLAERRGLPLVQMTMQFRGAGFASDRGGKQGTAGFTLAMLDEGAGEL
                     DALAFAARKESLGAVINASGTLDTASVFLSAIKPRLDDSLALFADVVTRPRLDDKDIE
                     RVRGKWLASIKQEKARPNTLQRRLVGPALFGPGHPYAIPLGGTGTEADIAALKRDDMT
                     RWLTQWIRPDNAILFVVGDTSLAELTPRLEKAFAAWKAPGTPLPAAAVAQVKPPARPR
                     VLLVDQPGALQANLMVAEVVPPSTDAGAIDLDIANGVLGGTFSSRINMNLREDKHWSY
                     GAFSSIGDALGQRVWATVAPVQIDKTVESIKEMRREVVDYVTGKAPARPEELAKLQAQ
                     RIRALPGSYETGNAVLRTLSDNALYGRPDDYPQQRANRISALTLDALKQAITAVQPGA
                     LTWIIVGDLQKIEKPVRALELGDLKVVDADGKPLR"
     gene            complement(3297..3689)
                     /locus_tag="VF516_11385"
     CDS             complement(3297..3689)
                     /locus_tag="VF516_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108324.1"
                     /translation="MIQANLSKAARKAIEMQPGLENFFNEAQRRNYERGVAKGKAEGR
                     AEGKAEGRAEGVAVALLKILERRRLTPTAEQRRRISVCTDLGVLERWLDRSLVVGSVA
                     ELLAEPARARRNGHRVARAVTNGHGRRK"
     gene            complement(3995..4843)
                     /locus_tag="VF516_11390"
     CDS             complement(3995..4843)
                     /locus_tag="VF516_11390"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HEX8108325.1"
                     /translation="MTTHRSITRSTTTYRTGLALAAIACAGTGLLGCAEAAEEAGPSG
                     DITHPEDDVPGSQVRIHEGTEGPEGPVLETAVVQTPGMDVSSHQGAVNWSTAWANGAR
                     FAYVKATEGTTYQNPDFAQQYNGSFNIGMIRGAYHFALPDRSSGATQGAFFVSHGGGW
                     SADGKTLPPAIDLEYNPYGASCYGLSQSAMVAWIHDIANTVKSRTGRDPVFYTSTSWW
                     TLCTGNNAGFSANPLWIPRYASSVGTLPAGWTFQTIWQFADSGVFPGDQNRFNGALDR
                     VQAFAL"
     gene            complement(5174..>5804)
                     /locus_tag="VF516_11395"
     CDS             complement(5174..>5804)
                     /locus_tag="VF516_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108326.1"
                     /translation="PLSWAAWLVNAEARVAWTADSDAGAQRSAWAPHAGEASLSFRDI
                     VVPASRGLREVEARLLIPYYDLSRTVRIDREVERMPERMPDGTPERMPEAMIERFDAQ
                     RSAARRHVQERIEALARVRIEPRIPGRRYRNVHVACLLERQTTERIALPAWTLAYRFR
                     GHPYRAIVHGQRAEVVFGRSPVDWSKVGWLVVMLAVLAAAAVALWLALR"
BASE COUNT          817 a         2051 c         2022 g          914 t
ORIGIN      
        1 agtgatcaat ggtatgcgct tcatgggata acctcggcag caaacaaagc aaggaccaga
       61 cccgaagtcg cttcgagccc aagcgcgtga atcaacgcag gtgccgtgtc ggagagcacc
      121 ggcgcgtggg taaccgcgcc ccacggcgac tcgccaccgc gcaagccgcg tacgtggtgg
      181 acgacacgcg ttgtgtaggg ttctcacacc gagaagcgct gcgcgagggc aatctgcctc
      241 gacgcggagc cgcgctgcga agcagcttcg cgtggcggtg cgccccgtca ccgcgagcgg
      301 cggttcagcg cagcggcttg ccgtcggcgt cgaccacctt gagatcgccg agctcgaggg
      361 cgcgcaccgg cttctcgatc ttctggaggt cgccgacgat gatccaggtc agcgcgccgg
      421 gctggaccgc cgtgatcgcc tgcttcagcg cgtcgagcgt gagcgcgctg atccggttgg
      481 cgcgctgctg gggatagtcg tcgggccggc cgtacagcgc gttgtcggac agcgtccgca
      541 gcaccgcgtt gccggtctcg tacgagccgg gcagcgcgcg gatccgctgg gcctgcagct
      601 tcgcgagctc ctcgggcctg gccggcgcct tgccggtgac gtagtcgacc acctcgcgcc
      661 gcatctcctt gatcgactcc accgtcttgt cgatctgcac cggcgcgacc gtcgcccaca
      721 cgcgctggcc gagcgcgtcg ccgatgctgg agaacgcgcc gtacgaccag tgcttgtcct
      781 cgcgcaggtt catgttgatc cgcgagctga acgtaccgcc cagcacgccg ttggcgatgt
      841 cgagatcgat cgcgccggcg tcggtgctgg gcggcacgac ctcggcgacc atcaggttgg
      901 cctgcagcgc gccgggctgg tcgaccagca gcacgcgcgg cctggccggc ggcttgacct
      961 gcgccaccgc cgcggcgggc agcggcgtgc ccggcgcctt ccacgccgcg aacgccttct
     1021 ccagccgggg cgtcagctcg gcgaggctgg tgtcgccgac tacgaacagg atggcgttgt
     1081 ccggacggat ccactgggtc aaccagcgcg tcatgtcgtc gcgcttgagc gcggcgatgt
     1141 cggcctcggt gccggtgccg cccagcggga tcgcgtaggg gtggccggga ccgaacagcg
     1201 ccgggccgac cagccggcgc tgcagcgtgt tgggccgcgc cttctcctgc ttgatcgacg
     1261 ccagccactt gccgcgcacg cgctcgatgt ccttgtcgtc gagccgcggc cgcgtgacca
     1321 cgtccgcgaa cagcgccagc gagtcgtcga gccggggctt gatcgcggac aggaacacgc
     1381 tggcggtgtc gagcgtgccg ctcgcgttga tcaccgcgcc gagcgactcc ttgcgggccg
     1441 cgaacgccag cgcgtcgagc tcgcccgcgc cctcgtcgag catcgccagc gtgaacccgg
     1501 cggtgccctg cttccccccg cgatccgacg cgaaccccgc gccgcggaac tgcatcgtca
     1561 tctggaccag cggcaggccg cggcgctcgg cgagcacgac ctggacaccg ttggacagcg
     1621 tcgcgcgcgc caccgccggg aaccgcagcg acgggaagct gtcggtcttc ggcacgccga
     1681 tcgagcggtc gacggtctgc ggcaccgcct tgagcccccg ggccggcggc gcgacctggg
     1741 tcggcggcag gttggggacc gccggctcct cgaccaccgg cttgcgctcc cccggatcga
     1801 tctgcagcgt gaacgcgccg cgcgccaggt acttgccggc gacctccttg agctgcgccg
     1861 cggtggcgct ctggaggttc gccagcgtcg tccggtagca cgccgggttg ccggtgaaca
     1921 cctggcactg ggccagcacg tcggccttgc cgccgaaccc gccgatccgc tcgacgcggc
     1981 ggatgaagcc cgccagcacc gccgcgcgcg cctgctcgac ctcggcggcg gtcggcccct
     2041 cggtgatcag cttgccgatc tcctcgtcga tcgcggcctc gaccttggcc gggtcgacgc
     2101 cggccttgac gtcggcctgg gcgatcaccg ccccaccgat ctcctgcagc tgcacgaacg
     2161 cacccacgct gtcggccagc ttctcggtga acgtcagccg cttgtcgagc cgcgacgact
     2221 tcgagccgcc gagcacctgc cccagcagca acagtcgatc gctgtccgcg ctgccgaact
     2281 gcggcccgtt ccacaccttg tagatccggg tctgcggcac gttgtcggtc gccgtctcgc
     2341 gcgtcgccgt ggtccgcagc gccggcttcg gcgacagcct ggccaggttc gccgacggcg
     2401 cgatgtcgcc gaagtacctg gtcaccttct ccttggcggt cgccaggtcg atgtcgccgg
     2461 cgagcaccag caccgcgttg ttcgggccgt accagctgcg gaaccagttc ttgacgtcgt
     2521 ccagcttcgc cgcgtcgagg tcgttcatcg agccgatcgt cgagtggtgg tacgggtgat
     2581 cctccggata gatcgccttc aggatcatcg tgaacgcccg cccgtagggc tggttctcgc
     2641 cctggcgctt ctcgttctgg accaccccgc gctgctcgtc gagcaggtgc tgatcgatcg
     2701 cgccgatcag gtggcccatc cggtcggact ccatccacag cgcgacgtcg accgccgtgg
     2761 tcggcacgtt ctcgaagtag ttggtgcgat ccccgttggt cgtgccgttc tggtcggtcg
     2821 cgccgaccag ctcgaacggc tcgaagaact cgcccttgaa gttctccgag ccctggaaca
     2881 tcaggtgctc gaacaggtgg gcgaacccgc tgcgcccttt cgcctcgttc ttggacccga
     2941 cgtggtacca gatgttgacc gcgatgatcg gcgccttgcg gtcggtgtgg accagcacgc
     3001 gcagcccgtt gggcagcacg aactgctcga acgggatgtc gacggtgatc gcccctccgc
     3061 gcggctggcc gtgcaccggc ggcggatccg ccgcgacgag cccggcggtg acaacgctac
     3121 agatcaggac cgtgaatcga gatgacgcgc gcatcgttcc ccctgtgcag cgcgcgcagc
     3181 atatcggttc gccgccccgt ccacgagcgg ctcgcctgcg acagccccgc gacgccggtg
     3241 acgcgcaggg tcatcggcga ggaggcggcg ccccgaccgt cgtcaccgtc gcgatgtcac
     3301 ttgcgccgac cgtgaccgtt cgtcacagcg cgggcaactc gatggccgtt ccgtcgagca
     3361 cgtgcgggct cggccaggag ctccgcgacc gagcccacca ccagggaccg atctagccag
     3421 cgttccagca ccccgagatc agtgcacacg ctgatgcggc gtcgctgctc ggcggtaggc
     3481 gtcagccggc gtcgttccaa gatcttcagc agtgccacgg ccacgccttc cgcccggcct
     3541 tccgccttgc cctcagcccg gccttccgcc ttccctttgg ctacaccgcg ctcgtagttc
     3601 cggcgttgcg cctcgttgaa gaagttctcg agcccgggtt gcatctcgat cgcctttcgt
     3661 gccgccttgc tcaggttcgc ctggatcaac agggagtata gcagccgttg ctcctcgggg
     3721 aacggcaaga tcgcccgggc ggccgcgctg ccgatggcag cggcggtgct gaccgcgcct
     3781 cgaccgtgga ccggcgacga acgccggacg gtcgcgcacc agcttgatca caccctcatg
     3841 cagcaacgac accatgtgat acgcgagtaa cctagtcgcg tcgcgaggca gtggcaactt
     3901 ctatcgccac caatcgaacg aagcatcgga tccggaatgc gtacgatgct gtcgtccgga
     3961 actcggacgc ttgcgacagc gcgcgacggg gcgatcagag cgcgaaggcc tggacgcggt
     4021 cgagcgcgcc gttgaaccgg ttctgatcgc cggggaacac gccggagtcg gcgaactgcc
     4081 agatggtctg gaaggtccag cccgccggca gggtgccgac gctggacgcg tagcgcggga
     4141 tccacagcgg attggcgctg aagccggcgt tgttgccggt gcacagagtc caccagctgg
     4201 tgctggtgta gaataccgga tcgcggccgg tgcgtgactt gaccgtgttc gcgatatcgt
     4261 ggatccacgc gaccatcgcg ctctgcgaca ggccgtagca gctcgcgccg tacgggttgt
     4321 actcgaggtc gatcgcgggc ggcagggtct tgccgtcggc cgaccatcca ccgccgtggc
     4381 tgacgaagaa cgcgccctgg gtcgcgccgc tcgagcgatc gggcagcgcg aagtggtagg
     4441 cgccgcggat catgccgatg ttgaacgagc cattgtactg ctgggcgaag tcggggttct
     4501 ggtaggtcgt gccctcggtc gccttgacgt acgcgaaccg cgcgccgttg gcccacgccg
     4561 tggaccagtt gaccgcgccc tggtgcgagc tgacgtccat gcccggggtc tggacgacgg
     4621 cggtctcgag cacggggccc tcggggccct cggtgccctc gtggatgcgg acctgcgagc
     4681 cggggacgtc gtcctcgggg tgggtgatgt cgccggacgg cccggcctcc tcggcagcct
     4741 ctgcgcatcc gagcaggccg gtgccggcac acgcgatggc cgcgagcgcc aggccggtgc
     4801 ggtacgtcgt tgtcgacctc gttattgatc gatgcgtcgt catcacgatc tcctttggca
     4861 gtggggcact cgcagaaacc gcggatcgcg cccgagcggt cggtcgctct gcgctcccgc
     4921 caccgcgagt cgcccgcccc gttccaggat ccgtgccagt acgctgccgg ggtgcgcggt
     4981 cttccgcgcg gggcggatgc gtggaaaatt gcatcgaatc gggcggtccg acaggtcggg
     5041 cgccgcgtcg actgcacacc gcagtcggca ggggcctcac cgtcgtcgag gccagtggaa
     5101 cctccacccc cagggtcggt gtcgcgcacg gtggtgcgcg agcgtacgtc gaggcgcggg
     5161 agcgacgcgc gcgctaccgg agggcgagcc agagcgcgac ggcggcggcc gcgaggacag
     5221 cgagcatcac gacgagccag ccgaccttgc tccagtcgac gggcgagcgg ccgaacacga
     5281 cctcggcgcg ctgaccgtgg acgatcgcgc ggtaggggtg gccgcggaag cgatacgcga
     5341 gggtccacgc cggcaaggcg atccgctcgg tggtctggcg ctccagcaga cacgcgacgt
     5401 ggacgttgcg gtagcgccgg ccggggatcc ggggctcgat gcggacccgg gccagcgcct
     5461 cgatgcgctc ctggacgtgg cggcgcgccg ccgagcgctg ggcatcgaat cgctcgatca
     5521 ttgcctctgg cattctctcc ggcgttccgt ccggcatccg ctccggcatc cgctcgacct
     5581 cgcgatcgat gcggaccgtc cggctcaggt cgtagtacgg gatcaggagg cgcgcctcga
     5641 cctcgcgcag gccgcgcgac gcgggcacga cgatgtcgcg gaacgacagc gaggcctcgc
     5701 cggcgtgcgg cgcccacgcc gagcgctgcg ccccggcgtc ggagtcggcg gtccaggcga
     5761 cgcgggcctc ggcgttgacc agccaggccg cccacgacag cggg
//
LOCUS       DASVOR010000576         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_297687,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000576 DASVOR010000000
VERSION     DASVOR010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_297687"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VF516_11400"
     CDS             <1..488
                     /locus_tag="VF516_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108327.1"
                     /translation="TAELGGQLTLHFQGRGYGEMWEAFALAGDRRTAGPLVLDADPVT
                     PGVQAQSYPGVSNLENYLETSARLAVRARLGARLSFAAFTQLAFRTDHVISFAEAGVD
                     LPTCPTGAPRCETEDNTVVTPGTAEVNPLHVKKIDLVGHRYRAEAGRGYVVGLEAHLA
                     F"
     gene            568..1728
                     /locus_tag="VF516_11405"
     CDS             568..1728
                     /locus_tag="VF516_11405"
                     /inference="COORDINATES: protein motif:HMM:NF024668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEX8108328.1"
                     /translation="MATIYLSSTYTDLRQHREAVCQQLRRMKHVVTAMEDYVARDDRP
                     MDACIRDVVQSDLYVGLFAWRYGHIPKAGNPSSLSITELEYRAAEKHQIPRLVFLLDE
                     AAAWPPNWLDSHTGDGERGERIQKLRAELKENRLESFFTSPEDLAAKVGAALHVAGTV
                     TDASDAAFDLAEIVGADVIDNPEMMFSHSTLPYLVERIAQLGDAPLLKIDLRDGAYWW
                     STRLYALATLAHEYTAVEWLLFLDRGTEYVGMIRPGDLRRALASSHPEIEEHYRLAHI
                     PPMFPGMDPGQRAAQVLNALTARFAEHPGGEQALRFVVDASWLSTKVPGLRTTHVERA
                     GVFDPLATFQLLQESTPFVPVTKGKQLLKVIDRVGVATELARMVVERRLGRT"
BASE COUNT          305 a          643 c          611 g          305 t
ORIGIN      
        1 tcaccgccga gctcggcggc cagctcacgc tccacttcca gggccgcggc tacggcgaga
       61 tgtgggaggc gttcgcgctc gccggcgatc gccgcaccgc cggcccgctc gtgctcgacg
      121 ccgatccggt caccccgggc gtccaggcgc agagctaccc cggcgtgtcc aacctcgaga
      181 actacctcga gacctcggcc cggctcgccg tgcgcgcccg gctcggcgcg cgcctgtcgt
      241 tcgccgcctt cacccagctc gcgttccgca ccgaccacgt gatcagcttc gccgaggccg
      301 gcgtcgacct gccgacctgc ccgaccggcg ccccgcggtg cgagaccgag gacaacaccg
      361 tcgtcacccc cggcaccgcc gaggtcaacc cgctgcacgt caagaagatc gacctggtcg
      421 gccaccgcta ccgcgccgag gccggccgcg gctacgtcgt cggcctcgag gcccacctgg
      481 cgttctagaa caccggtcct cgccgcccga catttcggtt gtccaggggc cgcggagtcg
      541 ttagtgttcg accgaggagg aaccggcgtg gcgacgatct atctgtcttc gacctacacc
      601 gatctgcggc agcatcgcga ggcggtgtgc cagcagcttc gccggatgaa gcacgtcgtc
      661 accgcgatgg aggattacgt cgcgcgagac gaccggccga tggacgcatg cattcgcgat
      721 gtcgtacaga gtgatctcta cgtcggtctg tttgcctggc gctacggtca tatcccgaaa
      781 gcaggcaatc cctcatcgct gtcgatcacc gagctcgagt accgtgctgc cgagaagcac
      841 cagataccga ggctcgtgtt cctgctggac gaggccgcag cctggccccc gaactggctc
      901 gattctcata ccggggacgg cgagcggggc gagcgtatcc agaagctccg ggcagagctg
      961 aaggaaaacc gcctggagag cttcttcacc tcgccggagg acctcgcagc caaggtgggc
     1021 gccgcgttgc atgtggcggg cactgtgacg gatgcttccg atgcagcctt cgacctggca
     1081 gagatcgtcg gggctgacgt gatcgacaac cccgagatga tgttcagcca ttctactctg
     1141 ccctaccttg tcgagcggat tgcacagctc ggcgacgcgc cgctcctcaa gattgacctg
     1201 agggatggcg cttactggtg gtcgacgcgg ctctacgcgc tggcgacact cgcgcacgag
     1261 tacaccgcgg tggagtggtt gctgttcctc gatcgaggaa cggagtatgt cgggatgatc
     1321 cggccgggtg atctgcggcg ggccctggcg tccagtcacc ccgagatcga agagcattac
     1381 cggcttgcac acattccacc gatgttcccc ggaatggatc ctggacaacg cgccgcgcag
     1441 gtcctcaacg cgctgacggc gcggttcgcc gagcatccag gcggcgaaca ggccctgcgc
     1501 tttgtcgtgg acgcatcgtg gctctccacg aaagtccccg ggctgcgcac gacgcacgtg
     1561 gagcgagcag gcgtcttcga tccacttgca acgttccagc tgctccagga gagcacgcca
     1621 ttcgttccgg tcacgaaggg caagcagctt ctgaaggtca tcgatcgggt tggcgtggcg
     1681 accgagctcg cccggatggt tgtagaacgg cgcctgggac ggacctgaga cgaacccagc
     1741 acccctccgg acccgtcacg cggggccccg actctcgccg gtggtgacgc cgcccgcgat
     1801 caggtgggcg aggcgcagcg gctcgggcac gttgccgtgc agcgtggtcg cggccaggag
     1861 cgcg
//
LOCUS       DASVOR010000577         3650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_34442,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000577 DASVOR010000000
VERSION     DASVOR010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34442"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VF516_11410"
     CDS             <1..468
                     /locus_tag="VF516_11410"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEX8108329.1"
                     /translation="DLDWDIVAAGVRATYGEARRACRAAKRSRSWFHAWRRRSKELGY
                     QLELIARHAGPRLTELRDEVSGATDVLGPGVDLIMVRDFVVTYGQGIAPEALGRLRDT
                     IHHQLDEMMKTARKAGREAFRAKPRKLEKRLTKSVRRDLAPPEDGNGAPEIQA"
     gene            550..864
                     /locus_tag="VF516_11415"
     CDS             550..864
                     /locus_tag="VF516_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108330.1"
                     /translation="MGKGDSRPPQQSAATKPAGPEPARIPKSSTPPLTTSRTTTLDDP
                     LTTSLLAEIARRSTTVEMSPAQLAQAQARAQAPGEPEPEPEPGENRDPDDPPTIRPDD
                     PA"
     gene            916..2103
                     /locus_tag="VF516_11420"
     CDS             916..2103
                     /locus_tag="VF516_11420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEX8108331.1"
                     /translation="MSKVLIIGAGGVGGVVAHKCAMDRQTFSDVLLASRRLASCDKLA
                     AEIQHMHGRAIRTAQVDADDVAATTRLIADYRPAVVINVALPYQDLHIMDACLAAGVD
                     YLDTANYEPPDVAKFEYKWQWAYRERFAAAGRMALLGSGFDPGVTNVFCAYVQKHLLD
                     EVHYVDIVDCNAGSHGKAFATNFNPEINIREVTARGRYWEDGQWKETDPLSVAKIFDY
                     PGVGERKSFLLYHEELESLVLHLKGLRRIRFWMTFGEAYLKHLEVLQNVGMTRIDEVE
                     FEGHKIVPIKFLRALLPDPASLAAGYTGKTSIGCLCEGVKDGKRKRYFIYNICDHAET
                     YREVRAQAVSYTTGVPAVTGAAMMVTGAWKGAGVFHMEQMDPDPFLADVARRGLPWHV
                     EER"
     gene            2106..3290
                     /gene="nspC"
                     /locus_tag="VF516_11425"
     CDS             2106..3290
                     /gene="nspC"
                     /locus_tag="VF516_11425"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0008216 - spermidine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEX8108332.1"
                     /translation="MSAGAALGADVARAIDVRDLETPCFVTDLGALEANLRILADIQQ
                     RARCTILLALKGFAQWSTFPLVKRYLAGATASSVAEARLAREELGGEVHAYAPAYPAG
                     EIRELVTLADHIVLNTPGQWRRHRRAIEQARRAGRSISCGLRVNHEHREVDVELYDPA
                     GPCSRLGTTRGNLAPGDLDGLDGLHFHTLCQAGSEALERALAAFERQFGEFIPQMDWV
                     NFGGGHHITRPDYDRDQLVHLIQGFQARWNVEVYLEPGEAIALGAGVLVASVLDVLHN
                     GMAIAILDTSATAHMPDVLEMPYRPVIVGAGEPGALPHTYRLGGMTCLAGDVIGDYSF
                     ERPLAIGDKLVFLDMAHYTMVKTTTFNGVRLPSIATHDPATGQITVHRRFGYRDYRDR
                     LS"
     gene            complement(3238..>3650)
                     /locus_tag="VF516_11430"
     CDS             complement(3238..>3650)
                     /locus_tag="VF516_11430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108333.1"
                     /translation="RLLARHGEHRLMIAAGVACAVLFAGWLAAPSAWLAVVLMVPVGA
                     TAAPLYPLASALAYARCPERSSIVLVGSHLFAPLTLALPWGLGWIADHAGVLAALALL
                     AAQPVGLVALAAVTSRAVTTVDRGSRDSRTGDAP"
BASE COUNT          579 a         1366 c         1248 g          457 t
ORIGIN      
        1 gacctcgatt gggacatcgt ggccgccggg gtgcgcgcga cctacggcga ggcccgccgc
       61 gcgtgccgcg ccgcgaagcg ctcgcggtcg tggttccacg cctggcgccg gcgcagcaag
      121 gagctgggct accagctcga gctgatcgcg cgccacgccg ggccgcggct gaccgagctg
      181 cgcgacgagg tgtcgggcgc gaccgacgtg ctcggccccg gggtcgacct gatcatggtg
      241 cgcgacttcg tcgtgaccta cggccagggc atcgcgcccg aggcgctcgg tcgcctgcgc
      301 gacacgatcc accaccagct cgacgagatg atgaagacgg cgcgcaaggc cgggcgcgag
      361 gcgttccgcg ccaagcccag gaagctggag aagcggctca cgaagtcggt gcgccgcgac
      421 ctggcgccgc ccgaggacgg caacggcgca cccgagatcc aggcctgaga cgatcgggcg
      481 taacgatctg cgtgaatccc cgagcctgcg gagctggggc accgcgcgga gagctgctag
      541 gatcgcggca tgggcaaggg agactcgcgg cccccacagc agtccgccgc gaccaagccg
      601 gccggaccag aaccggcccg cataccgaaa tcctccacac cgccgctgac gacgtcgcgg
      661 accacgacgc tcgacgaccc gctgaccacg agcctgctcg ccgagatcgc gcggcggtcg
      721 acgaccgtgg agatgtcgcc cgcgcagctc gcccaggccc aggcccgggc ccaggcgccc
      781 ggcgagcccg agcccgagcc cgagcccggc gagaaccgcg atcccgacga tcctccgacg
      841 atccggcctg acgacccggc ctgacgatcg gccggacggc acgaacgcca ccggcgtgtg
      901 ctagcttgcg cccccatgtc gaaggtcctc atcatcggcg ccggaggcgt cggcggcgtc
      961 gtggcccaca aatgcgcgat ggatcgccag acgttcagcg acgtcctgct cgccagccgc
     1021 cggctcgcga gctgcgacaa gctcgccgcc gagatccagc acatgcacgg ccgcgcgatc
     1081 cgcaccgcgc aggtcgacgc cgatgacgtc gccgcgacca cccggctgat cgccgactac
     1141 cggccggcgg tcgtgatcaa cgtcgcgctg ccctaccagg atctccacat catggacgcc
     1201 tgcctcgccg ccggcgtcga ctacctcgac accgccaact acgagccgcc cgacgtcgcg
     1261 aagttcgagt acaagtggca gtgggcgtac cgcgagcggt tcgccgccgc cggccggatg
     1321 gcgctgctcg gctcgggctt cgacccgggc gtgaccaacg tgttctgcgc ctacgtccag
     1381 aagcacctgc tcgacgaggt ccactacgtc gacatcgtcg actgcaacgc cggctcgcac
     1441 ggcaaggcgt tcgcgaccaa cttcaacccc gagatcaaca tccgcgaggt caccgcgcgc
     1501 ggccggtact gggaggacgg gcagtggaag gagaccgacc cgctcagcgt ggcgaagatc
     1561 ttcgactacc ccggcgtcgg cgagcgcaag agcttcttgc tctaccacga ggagctcgag
     1621 tcgctggtcc tccacctcaa gggcctgcgc cggatccggt tctggatgac gttcggcgag
     1681 gcgtacctca agcacctcga ggtgctgcag aacgtcggca tgacccggat cgacgaggtc
     1741 gagttcgagg gccacaagat cgtcccgatc aagttcctgc gcgcgctgct ccccgatccg
     1801 gcgtcgctcg ccgccggcta caccggcaag acgtcgatcg gctgtctgtg cgagggcgtc
     1861 aaggacggca agcgcaagcg ctacttcatc tacaacatct gcgatcacgc cgagacctac
     1921 cgcgaggtcc gcgcccaggc ggtgtcgtac accaccggcg tgcccgccgt gaccggcgcc
     1981 gcgatgatgg tgaccggcgc atggaagggc gccggcgtgt tccacatgga gcagatggat
     2041 cccgacccgt tcctcgccga cgtggccagg cgcggcctgc cctggcacgt ggaagagcgc
     2101 tagccatgag tgcgggagct gcgctcggcg ccgacgtcgc ccgcgcgatc gacgtgcgcg
     2161 atctcgagac cccgtgcttc gtgaccgacc tcggggcgct cgaggccaac ctgcggatcc
     2221 tcgccgacat ccagcagcgc gcgcgctgca cgatcctgct cgcgctcaag ggcttcgcgc
     2281 agtggtcgac gttcccgctg gtcaagcgct acctcgccgg cgcgaccgcg agctcggtcg
     2341 ccgaggcgcg gctggcgcgc gaggagctcg gcggcgaggt ccacgcctac gccccggcct
     2401 accccgccgg cgagatccgc gagctggtca cgctcgccga ccacatcgtg ctcaacacgc
     2461 ccggccagtg gcggcgccat cgccgggcga tcgagcaggc gcggcgcgcc ggccggtcga
     2521 tctcgtgcgg cctgcgggtc aaccacgagc accgcgaggt cgacgtcgag ctctacgatc
     2581 cggcggggcc gtgctcgcgg ctgggcacca cgcgcggcaa cctcgccccc ggcgacctcg
     2641 acggcctcga cggcctgcac ttccacacgc tgtgccaggc cggcagcgag gcgctggagc
     2701 gcgcgctggc cgcgttcgag cgccagttcg gcgagttcat cccgcagatg gactgggtca
     2761 acttcggcgg cggccaccac atcacccgcc cggactacga tcgcgaccag ctggtccacc
     2821 tgatccaggg cttccaggcc cggtggaacg tcgaggtcta cctcgagccc ggcgaggcga
     2881 tcgcgctcgg cgccggcgtg ctggtcgcca gcgtgctcga cgtcctgcac aacggcatgg
     2941 ccatcgcgat cctcgacacc tcggcgaccg cccacatgcc cgacgtgctc gagatgccgt
     3001 atcgcccggt gatcgtcggc gccggcgagc cgggcgccct cccgcacacc taccggctcg
     3061 gcggcatgac ctgcctggcc ggcgacgtga tcggcgacta cagcttcgag cgcccgctcg
     3121 cgatcggcga caagctggtg ttcctcgaca tggcgcacta cacgatggtc aagaccacca
     3181 cgttcaacgg cgtgcggctg ccgtcgatcg cgacccacga tccggcgacc gggcagatca
     3241 cggtgcatcg ccggttcggc tatcgcgact accgcgatcg actgtcgtga cggcgcgcga
     3301 ggtcaccgcc gccagcgcga ccagcccgac cggctgggcc gcgagcagcg cgagcgccgc
     3361 cagcacgccg gcgtgatcgg cgatccagcc gagcccccag ggcagcgcga gcgtgagcgg
     3421 cgcgaacagg tggctcccga ccagcacgat gctcgaccgc tcgggacagc gcgcgtaggc
     3481 cagggccgac gccagcgggt acagcggcgc cgcggtcgcg ccgactggga ccatcagcac
     3541 gaccgcgagc cacgcgctcg gcgcggcgag ccagccggcg aacagcacgg cgcacgcaac
     3601 cccggcggcg atcatcagcc ggtgctcgcc gtggcgcgcc agcaggcggt
//
LOCUS       DASVOR010000578         2834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_531489,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000578 DASVOR010000000
VERSION     DASVOR010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_531489"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..861
                     /locus_tag="VF516_11435"
     CDS             136..861
                     /locus_tag="VF516_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HEX8108334.1"
                     /translation="MTDPPSAHARLARFPITQKWPAQHPERLQLYSLPTPNGVKVSIM
                     LEEIGLPYEAHLVSFDTQDQMSPEFGSLNPYHKIPAMIDPDGPGGEPLPLFESGAILI
                     YLAEKTGQLLSTAPAERYETIQWLMFQMGGLGPMFGQLGFFHKFAGKDYEDKRPRDRY
                     VGESRRVLRVVDGRLAGRAWIMGDRYSIADIAIFPWVRNLLGFYDAGEIVGISEFPHV
                     TRALAAFVARPAVIRGLAVPARA"
     gene            complement(866..2008)
                     /locus_tag="VF516_11440"
     CDS             complement(866..2008)
                     /locus_tag="VF516_11440"
                     /inference="COORDINATES: protein motif:HMM:NF005757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8108335.1"
                     /translation="MSIQVRSASRGSLALLLVPLALCALLGSVARAAEPEKKPGPAPG
                     SFTIDNFQLESGVTLPKAIVVYGTYGTLDAAKDNAVLLPSHYMAQSDGYDWLIGEGRA
                     LDPKKHFLIMTELFGNGRSSSPSNTPEPFHGPHFPVTTIRDNVEAVRRLLEDRLGVHH
                     LKAVVGFSMGAEQAFQWAVSHPRFADKIVATAGTARCWPHGVVRLDGQIRAITLDPAF
                     KGGDYTAQPARGIETTGIVWLGWLYSQEWWKRELWKKTEPSAAGMPLAEYIEKQAKEF
                     FAGLDANDLILQMRTWQVHDVGTTPGFGGSTENALRSISAPVLYMPSETDLYFPLGDA
                     RQEAKWIRKVTLAPIPSLWGHPAGVGATPEDLAFIERKLRAFLGTP"
     gene            2253..>2834
                     /locus_tag="VF516_11445"
     CDS             2253..>2834
                     /locus_tag="VF516_11445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8108336.1"
                     /translation="MRVLLSTIGTRGDVQPLVALALALKALGQEVRLCVPPDFRDWIE
                     SLGLPVAPIGPELRSTANPSAPRTTPAEVRRMIEGTVATQFETIAAAARGHDLIVGAT
                     ALQIAAPSVAETMGIPYVFAAYCPAVLPSPHHAPPVSPTRGDTPPPAMADHRELWDQD
                     ARRWNDPWGPLLNAQRAARGLSPVRDVRSHVLTD"
BASE COUNT          421 a          996 c          972 g          445 t
ORIGIN      
        1 cccgcgcgcg gctcgtgatc ctgcagatcg ccagcggcct gccgcccgac gaggccgccg
       61 cctggcgcgc gcgcgtggcc gccgccgaag ggcagagcgc atgacaccgc cccgcgagat
      121 cggctaagca tcggcatgac cgatccgccg tccgcccacg cgcgcctggc gcgctttccg
      181 atcacccaga agtggcccgc gcagcacccc gagcggctgc agctctacag cctgcccacc
      241 cccaacggtg tgaaggtgtc gatcatgctc gaggagatcg gcctgccgta cgaggcccac
      301 ctggtgagct tcgacaccca ggaccagatg tcgcccgagt tcgggtcgtt gaacccgtac
      361 cacaagatcc cggcgatgat cgatcccgac gggcccggcg gcgagccgct gccgctgttc
      421 gagtccggcg cgatcctgat ctacctggcc gagaagaccg gccagctgct gtcgaccgcg
      481 cccgccgagc gctacgagac gatccagtgg ctgatgttcc agatgggtgg gctcggtccg
      541 atgttcggac agctcggctt cttccacaag ttcgccggca aggactacga ggacaagcgg
      601 ccgcgcgatc gctacgtcgg cgagtcgcgc cgcgtgctgc gggtcgtcga cggccggctc
      661 gccggtcgcg cgtggatcat gggcgatcgc tacagcatcg ccgacatcgc gatcttcccg
      721 tgggtgcgca acctgctcgg gttctacgac gccggcgaga tcgtcggcat ctccgagttc
      781 ccccacgtca cgcgcgcgct cgccgcgttc gtcgcccgcc ccgccgtgat ccgcggcctc
      841 gccgtccccg cgcgcgcgtg atcgctcagg gcgtcccgag gaacgcgcgc agcttgcgtt
      901 cgatgaaggc gaggtcctcg ggggtggcac ccacgccggc ggggtgtccc cagagcgacg
      961 ggatgggcgc gagcgtgacc ttccggatcc atttggcctc ctggcgcgcg tcgcccagcg
     1021 gaaagtacag atcggtctcc gagggcatgt agagcaccgg agcggagatc gaacgcagcg
     1081 cgttctcggt gcttccgccg aaccccggcg tggtccccac gtcgtggacc tgccaggtcc
     1141 gcatctgcag gatgagatcg ttcgcgtcca gtcccgcgaa gaactccttg gcctgtttct
     1201 cgatgtattc tgccaggggc atgccggcgg ccgacggttc ggtcttcttc cacagctcgc
     1261 gcttccacca ctcctgggaa tacagccagc ccagccacac gatgccggtg gtctcgatgc
     1321 cgcgcgcggg ctgcgcggtg tagtcgcctc ccttgaacgc cgggtcgagg gtgatggcgc
     1381 ggatctggcc gtcgaggcgg acgacgccgt gcggccagca ccgggcggtg ccggcggtgg
     1441 cgacgatctt gtcggcgaac ctggggtggc tcacggccca ctggaacgcc tgctccgcac
     1501 ccatcgagaa ccccaccacg gccttgaggt ggtggacgcc caggcggtcc tcgagcagcc
     1561 gccgcaccgc ctcgacgttg tcgcggatgg tggtcaccgg gaagtgtgga ccgtggaacg
     1621 gctcgggcgt gttgctcggg ctcgacgacc gcccgttgcc gaacagctcg gtcatgatca
     1681 gaaagtgctt cttggggtcc agcgcgcggc cttctccgat cagccagtcg tagccgtcgc
     1741 tctgggccat gtagtgcgac ggcagcagca cggcgttgtc cttcgcggcg tcgagcgttc
     1801 cgtaggtgcc gtagaccacg atcgccttgg gaagcgtgac gccactctcg agctggaagt
     1861 tgtcgatggt gaacgagcca ggcgcgggcc ccggcttctt ttcgggctcg gcggctcgcg
     1921 caaccgagcc gagcagcgcg cacagcgcga gcggcacgag aaggagagcg aggctccccc
     1981 gggaagcgga cctgacctgg atgctcatgg gggcggagct tcccacacgc gccgcgtccc
     2041 gggtgccggg ttgccgggtt gccgggttgc cgggttgccg ggttgtgatc accccccgcc
     2101 gcgcgaaccc gtccttcgag gcgggttccc gcacgccttc ttcccactca catactcgca
     2161 aaaccaggcc gtcgttacaa gcttgtcaag tacgtcgagg ttcgcgatca cgtgggcatg
     2221 atgcaaacag ccaacacgga aggaggagtg tgatgcgcgt actgctatcg acgatcggca
     2281 ctcgggggga tgtccagccg ctggtggcgc tggcgctggc gctcaaggcg ctcggccagg
     2341 aggttcgcct gtgcgtaccg cccgacttcc gggactggat cgagagcctg gggctgcccg
     2401 tcgcgccgat cggacccgag ctgcgctcga ccgcgaaccc atcggcaccc cggaccacac
     2461 ccgcggaggt gcgccggatg atcgagggca cggtcgccac gcagttcgag acgatcgctg
     2521 cggcagctcg aggccacgac ctgatcgtgg gagcaacggc cctgcagatc gccgctccgt
     2581 ccgtggcaga gacgatgggc atcccgtacg tgtttgccgc ttattgcccg gcagttctgc
     2641 catcgccgca ccacgcgcca cccgtgagcc ccacgcgggg agacacgccg ccgcccgcga
     2701 tggccgacca ccgcgagctc tgggaccagg atgcgcggcg ctggaacgac ccgtggggcc
     2761 cgctgctcaa cgcgcagcgc gcagcgcgcg gcctgtcccc ggtccgggac gtgcgcagcc
     2821 acgtcctgac cgat
//
LOCUS       DASVOR010000579         3355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_356124,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000579 DASVOR010000000
VERSION     DASVOR010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3355
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356124"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VF516_11450"
     CDS             complement(<1..305)
                     /locus_tag="VF516_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108337.1"
                     /translation="MNIKAFFVAALSSALDLGIAVPDDVLRHVTPDVLAAHLPRPLWA
                     RLLTACVGAPRVDAQLVVETIGVPNLCEHVPAPIIWACIEELALRALGGVVIAAPGPA
                     "
     gene            complement(302..607)
                     /locus_tag="VF516_11455"
     CDS             complement(302..607)
                     /locus_tag="VF516_11455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108338.1"
                     /translation="MSNDQRQAWFARVMESGLENDIFAPSDVLAHATPDVLASHLPAE
                     LLSKVLASSLAAGSMTPERVLETVTPEVMAKHLPHEVLWQCIAAAAARAGVDKAVTS"
     gene            744..1196
                     /locus_tag="VF516_11460"
     CDS             744..1196
                     /locus_tag="VF516_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8108339.1"
                     /translation="MPEPRAKLSSGTLLYRFLAGTVEVLLVHPAGNYNRRAPWGIPKG
                     APDPDEPLEETARRETLEETGVRVAGNLVDLGHVDYTRSKKRVHAFAAPAPEGAVPRC
                     ASWEVDKAEFIEITRARRIIHPDQAVLLDRLQRYLAPEVQIPAEKTIA"
     gene            1766..3046
                     /locus_tag="VF516_11465"
     CDS             1766..3046
                     /locus_tag="VF516_11465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8108340.1"
                     /translation="MWDEKVLETKVDEALAAKAARPIRTPDSDDHLAAYFRQLAEHEL
                     LTPEDERELSQGIEDTEILTWERVLSRPEAIRPLLALIEPNLEQPVKFPKLQKVLDDM
                     LKSKRARKDDKLVKKLLVAAKEAAAQMRALDLDRVHIDAVVRELYRAREASIAGLETW
                     WTDGASKDAGAYVDEVRQAHRDAMNLRDEFVRANLRLVVTMARRYDRGGMPLADLIQE
                     GNLGLMHAVSRFDYRRGLRFSTYACWWIRHAIGRALADKARAVRIPVHMLEAQQQLEK
                     VRQALVGELGRPPTPQELAKAARVPLAKLNQMHRYIMGQPMSLDRPVHDDDDRSFGDT
                     MADPDSEEHSPADNLTTQTLTSQIKRLLHYLSPIEADVLTKRFGLGDDEERTFREIGD
                     QYHLSRERIRQIQNSALDKLKRALEREHRGHVEM"
BASE COUNT          550 a         1168 c         1163 g          474 t
ORIGIN      
        1 gccgggcccg gcgcagcgat cacgacgccg ccgagcgcgc gcagcgcgag ctcctcgatg
       61 caggcccaga tgatcggcgc cgggacgtgc tcgcacaggt tcggcacgcc gatggtctcc
      121 accacgagct gggcgtcgac ccgcggcgcg ccgacgcacg cggtgagcag ccgggcccac
      181 agcgggcgcg gcaggtgcgc cgcgagcacg tcgggcgtga cgtgccggag cacgtcgtcg
      241 ggcaccgcga tcccgagatc gagcgccgac gacagcgccg cgacgaagaa cgccttgatg
      301 ttcacgaggt caccgcttta tcgacgccgg cgcgcgccgc cgccgccgcg atgcactgcc
      361 acaggacctc gtgcggcagg tgcttggcca tcacctcggg cgtgacggtc tccagcacgc
      421 gctcgggcgt catcgatccc gcggccagcg agctggcgag gaccttcgac agcagctcgg
      481 ccggcaggtg gctggcgagc acgtcgggcg tggcgtgggc caggacgtcg ctcggcgcga
      541 agatgtcgtt ctccaggccc gactccatca cccgcgcgaa ccaggcctgg cgctgatcgt
      601 tgctcattcc ctcgctcctt ggatgctcgc tgtcgacgct acaccgtttg ccaggtaggt
      661 ggggggagca atctcgcgag atcgttggaa tcgactgcaa tagacttgca atggccgccg
      721 ctgtcttgca cggtcaacga gggatgccag agccccgagc aaagctgtcg tcaggcacct
      781 tgctctaccg attcctcgca ggaacggtgg aagtgctgct ggtgcacccg gccggcaact
      841 acaaccggcg cgcgccgtgg ggcatcccga aaggtgcgcc ggatcccgac gagccgctgg
      901 aggagaccgc gcgccgcgag accctggagg agaccggcgt gcgggtcgcc ggcaatctgg
      961 tcgacctcgg ccacgtcgac tacacgcgct cgaagaagcg ggtccacgcg ttcgcggcgc
     1021 cggcgcccga gggcgcggtc ccgcgctgcg cgtcctggga ggtggacaag gccgagttca
     1081 tcgagatcac ccgcgcccgc cgcatcatcc accccgatca ggcagtactc ctcgatcgcc
     1141 tgcagcgcta ccttgcgccc gaagtacaga tcccagcgga gaagaccatc gcctgaggcg
     1201 tacctccggc gaaatcgcag ggagatcggc gtgaggtggg cggcacatgc gcagcgcagg
     1261 gaggggggct gcgcgccccg ctcccgtcgg tatctcgaca ccccagggta ggcaggactg
     1321 tcacgaaaga cgacacggaa cgattcaccc tggacaattt ttgtggccat ggcacagtgc
     1381 gacgcttggg ctgtctcacc tgtaccccgg tgtgagggct agccaagccg ctgattctgc
     1441 gtggcgtgcc ccgacctgac cggtcggccc gttgtctcga agaagttcgg tgcgcaacgt
     1501 gcacgttgga acctgcgttg gaacctccgc gcgccctcca ctcgtagtcc ctttcgcagg
     1561 aatcacggcg cggatgcaac ggttgccgtt tgaacagcgt cttacccgag gatcacagga
     1621 gatacgcatg gccaccactc atcccccaag tcgccggcag cgcccaccgc gccgtacgac
     1681 cgcgagcatg gctcatgcgc gccatcggtc ggcgggagcg cggcagaacc gcacggcccc
     1741 cgaatccact gtggcggagc cgcccgtgtg ggatgagaag gtcctggaga cgaaggtcga
     1801 cgaggcgctc gcggccaagg cagcgcgtcc gatccggacg ccggactccg acgatcatct
     1861 cgccgcgtac ttccgtcagc tggcggagca cgagctgctg actcccgagg acgagcgcga
     1921 gctgtcccag gggatcgagg acaccgagat cctgacctgg gagcgcgtgc tgtcgcgtcc
     1981 ggaggcgatc cgcccgctgc tcgccctgat cgagcccaac ctcgagcagc cggtcaagtt
     2041 cccgaagttg cagaaggttc tcgacgacat gctcaagtcc aagcgcgcgc gcaaggacga
     2101 caagctggtc aagaagctcc tggtcgcggc caaggaagcg gcggcgcaga tgcgcgcgct
     2161 cgacctcgat cgcgtgcaca tcgacgcggt ggtccgcgag ctgtaccgcg cgcgcgaggc
     2221 ttcgatcgcc gggctcgaga cctggtggac cgacggcgcc agcaaggacg cgggcgccta
     2281 cgtcgacgag gtccgccagg cccaccgcga cgcgatgaac ctgcgcgacg agttcgtgcg
     2341 cgccaacctc cgcctggtcg tcaccatggc gcggcgctac gaccgcggcg gcatgccgct
     2401 cgccgacctg atccaggagg gcaacctcgg gctgatgcac gccgtgtcgc ggttcgacta
     2461 ccgccgcggc ctgcgattct cgacgtatgc ctgctggtgg atccgccacg cgatcggccg
     2521 tgccctggcc gacaaggcgc gcgcggtccg catcccggtg cacatgctcg aggcccagca
     2581 gcagctcgag aaggtccgcc aggcgctggt cggcgagctc ggccggccgc cgactccgca
     2641 ggagctggcc aaggccgcgc gggtcccgct cgccaagctg aaccagatgc atcgctacat
     2701 catgggccag ccgatgtcgc tcgaccggcc ggtccacgac gacgatgacc gcagcttcgg
     2761 cgacacgatg gccgatccgg acagcgagga gcactcgccg gcggacaacc tgaccaccca
     2821 gacgctgacg agccagatca agcggctgct gcactacctg tccccgatcg aggccgacgt
     2881 gctgaccaag cggttcgggc tcggcgacga cgaggagcgc acgttccgcg agatcggcga
     2941 tcagtaccat ctgtcgcgcg agcgaatccg ccagatccag aattctgcac tcgacaagct
     3001 gaagcgcgcg ctggagcgcg agcaccgcgg ccacgtcgag atgtgacgat cgggactggg
     3061 cgggcctggg cagatctcgg cgggtctcgg gggcccgggg tccggccgct cgggaaggtg
     3121 actggccggg ggtgatctgc ccgcccctac cgaggatctg cgcgctgcag ggccgccgca
     3181 cggcccgctg cgccggcggc tgcgctgtac cagcgcggtt ccgcctgtac cggtggccct
     3241 gccgtgacgt gcctcgcggc gtcgcagcag gccgactcga tggcgcttcc cgcgactggg
     3301 atgcccctgt cgctgcggca gtcgcaaggt gccggtgggg ttccggcggt accgg
//
LOCUS       DASVOR010000580         2716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_63780,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000580 DASVOR010000000
VERSION     DASVOR010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63780"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2480)
                     /locus_tag="VF516_11470"
     CDS             complement(<1..2480)
                     /locus_tag="VF516_11470"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8108341.1"
                     /translation="MDPELNELNEAGPDDEEVSLILRLETGAEPPPTVRVVSRFGPII
                     TVRCRRADILTTWASDRVISVKAPRAVYLPRPVRARRAPAELEEEAAADVEWDPEEEE
                     AIAEDAPAGHHPLPALPFDGRGVVVGICDWGFDFTHPNFRNPDGSTRLIALWDQRGTG
                     DPRAPAPYHRGRLFTPDAINAALAQPDPCAALGYHPASGDPNNTGSHGTHVSDIVAGN
                     RRYPGSQVGLASGAKLVFVHLGAQRLGELDNLGDSVGLLEGLDFVRRTAAAAGCPCVM
                     HLSAGKTGGPHCGVTLVERAVDALLQDTPGIVLVQSGGNYADAAMHTHARIGPDQRYV
                     IDWWIPPGDRTPNELEIWYSGMDVFDVTLVAPDGQEFSAPLAQRARLGDGLEAWGNFY
                     HRLHEPNSGFNHVAIFLYTAAPTGRWKVVVHGRDVLDGRLHAWIERDAGSRYQSRFSR
                     AQATSSYTTNTICNSFRAIAVGAYDATRPERPPGHFSSRGPTADGRQKPEIAAPGVRI
                     LAARSMPRDGWNGERKLVVKSGTSMAAPCVSGTVALMFQAAGRPLTIHEVRRILIGTA
                     DPLAGPRGRSSSQLGFGYLNTAAAVEAARRLGSGAAAPLLPAAAFPLEQDEAQDEAPI
                     ESTWIEEPLGEPAVAEQVVAPPGAIYPDDPQYRKEHKTELEYIDDSAGPCTFFNIVGD
                     PIHGDEAFRGLVTIAGEGDELFIVTWMFDAEMELIRDGWDQPTSDPARQNIRLAQRIV
                     AAAKRGAKISIIIWDPTAIFASSAALSETDESAGVPQQDLYLEVLGPQDWLDEKRKKY
                     AEAKRIRNAINKLRTDLRAISSDNEILLL"
     gene            complement(2480..>2716)
                     /locus_tag="VF516_11475"
     CDS             complement(2480..>2716)
                     /locus_tag="VF516_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108342.1"
                     /translation="MSAESGQGWQCLLKEDVDPETLRGPDGRLSDDAVVLRRVSRWGR
                     ATEREAIDEGYAGGPAEEDPPTGWAGGSAGGRHR"
BASE COUNT          338 a          951 c          998 g          429 t
ORIGIN      
        1 agtagcagga tctcgttgtc cgaggagatc gcgcgaaggt ccgtccggag cttgttgatc
       61 gcgttccgga tccgcttcgc ctcggcgtac ttcttgcgct tctcgtcgag ccagtcctgg
      121 gggccgagca cttccaggta aagatcctgc tgcggcacgc cggcgctctc gtcggtctcg
      181 ctgagcgctg cagagctcgc gaagatcgca gtcggatccc agatgatgat cgagatcttc
      241 gcaccgcgct tggccgctgc cacgatcctc tgggcgagtc gaatgttctg ccgtgcggga
      301 tcgctggtcg gctggtccca gccgtcgcgg atcagctcca tctccgcatc gaacatccac
      361 gtgacgatga acagctcatc gccttcaccc gcgatcgtga cgagacccct gaaggcttca
      421 tcgccatgaa tcggatcacc gacgatgttg aagaatgtac aaggcccggc gctgtcgtcg
      481 atgtactcga gctcggtctt gtgctccttg cggtactgcg gatcgtcggg gtagatcgct
      541 cccggcgggg cgacgacctg ctcggcgacc gcgggctcgc ccagcggctc ctcgatccac
      601 gtcgattcga tcggcgcctc gtcctgcgcc tcgtcctgct ccagcgggaa cgcggcggca
      661 gggagcagcg gcgcggcggc cccgcttccc agccggcgcg ccgcctcgac cgccgcggcg
      721 gtgttgaggt agccgaagcc gagctggctg gagctgcgcc cgcgcggccc ggcgagcgga
      781 tcggcggtgc cgatcaggat ccgccgcacc tcgtggatgg tcagcgggcg cccggcggcc
      841 tggaacatca gggccaccgt ccccgacacg cagggcgcgg ccatgctggt cccggacttg
      901 acgaccagct tgcgctcgcc gttccacccg tcgcgcggca tcgagcgcgc cgccaggatg
      961 cggacccccg gcgcggcgat ctccggcttc tggcggccat cggccgtcgg gccgcggctg
     1021 gagaagtgcc cgggcggccg ctcggggcgg gtggcgtcgt aggcccccac cgcgatggcg
     1081 cggaagctgt tgcagatcgt gttggtcgtg tagctcgacg tcgcctgcgc gcgggagaac
     1141 cgcgactggt agcggctgcc ggcgtcgcgc tcgatccagg cgtggagcct gccgtcgagg
     1201 acgtcgcggc cgtggacgac gaccttccag cgtccggtgg gcgcggcggt gtagaggaag
     1261 atggcgacgt ggttgaagcc gctgttgggc tcgtgcaggc ggtgatagaa gttgccccac
     1321 gcctccaggc cgtcgcccag ccgggcgcgc tgggccagcg gcgccgagaa ctcctgcccg
     1381 tcgggggcca ccagcgtcac gtcgaagacg tccatgccgc tgtaccagat ctcgagctcg
     1441 ttgggcgtgc ggtcgccggg cgggatccac cagtcgatca cgtacctctg gtccggcccg
     1501 atgcgggcgt gggtgtgcat ggcggcgtcg gcgtagttgc cgccgctctg gaccagcacg
     1561 atgccggggg tgtcctggag cagggcgtcg acggcgcgct cgaccagcgt gacgccgcag
     1621 tgcgggccgc cggtctttcc ggcgctgagg tgcattacgc aggggcagcc ggcggccgcc
     1681 gccgtgcgcc gcacgaagtc cagcccctcg agcaggccga ccgagtcacc caggttgtcg
     1741 agctcgccca ggcgctgcgc gccgaggtgc acgaacacca gctttgcgcc cgaggccagt
     1801 ccgacctggg atccgggata ccgccggttg ccggccacga tgtcggacac gtgggtgccg
     1861 tgcgagccgg tgttgttggg gtcgccgctg gccgggtgat agccgagcgc cgcgcacggg
     1921 tccggctggg ccagcgcggc gttgatggcg tcgggcgtga acaggcggcc tcggtggtag
     1981 ggcgcgggcg cgcgcgggtc gccggtgccg cgctgatccc acagcgcgat caggcgggtc
     2041 gagccgtcgg ggttgcggaa gttggggtgg gtgaagtcga agccccagtc gcagatcccg
     2101 accacgacgc cgcggccgtc gaagggcagg gcgggcagcg ggtggtggcc ggcgggcgca
     2161 tcctcggcga tggcctcttc ctcctcggga tcccactcga cgtcggccgc cgcctcctcc
     2221 tcgagctcgg cgggcgccct gcgcgcgcgg accgggcgcg gcaggtagac ggcgcgcggc
     2281 gccttcacgc tgatgacccg atcgctcgcc caggtcgtca ggatgtcggc gcggcggcag
     2341 cgcaccgtga tgatggggcc gaagcggctc acgacgcgca cggtgggcgg tggctcggcc
     2401 ccggtctcga ggcgcaggat caggctgacc tcctcgtcgt cggggccagc ttcgttcagc
     2461 tcgttgagct cggggtccat catcggtgcc tcccgcccgc tgatcccccg gcccatcctg
     2521 tgggcgggtc ctcctccgcc ggcccgccgg cgtacccctc gtcgatcgcc tctcgctcgg
     2581 tggcacgccc ccaccgcgac acccgccgca gcacgaccgc gtcgtcgctc agccgcccgt
     2641 cgggcccgcg gagcgtctcg gggtcgacgt cctccttgag caggcactgc cagccctggc
     2701 cgctctcggc ggacat
//
LOCUS       DASVOR010000581         3942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_356144,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000581 DASVOR010000000
VERSION     DASVOR010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356144"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..918
                     /locus_tag="VF516_11480"
     CDS             <1..918
                     /locus_tag="VF516_11480"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108343.1"
                     /translation="LSPGEILATGAEARAIAGGERRAVTYGDLRSGIERRLRNSLQDL
                     AWRIDVRVRWSDLVLPPEGRGRVEEFIARRAFAHKVYGEWGFGDRLERGKGSIALFSG
                     PPGTGKTMLAGLIAQGLGLDLYQVDLSQIQSRWIGETEKQLGRVFDLAERAHAVLLFD
                     EADSLLAKRTDVETSNDRYANANVNYLLQRLEQYTGVVVLTTNKDAALDEALQRRLTL
                     HLRLDVPEIPEREQLWRSFLPQGAPRDADLDVAALAREFELTGGLIKNVVVRAAFLAA
                     REDQKLGTGLLRRAATLELEDMGRVVRAA"
     gene            complement(922..3396)
                     /locus_tag="VF516_11485"
     CDS             complement(922..3396)
                     /locus_tag="VF516_11485"
                     /inference="COORDINATES: protein motif:HMM:NF040071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HEX8108344.1"
                     /translation="MMLRVPSPCSLWLAAAALAASGCDSNSSSTGSTAPPSQPVPQIK
                     TLSNRADMISGGDALVEIVLPPGATAATLHVTVGPPGKPDMVQDVSDRFTARADGRLI
                     GLVTGLAEGANTLSVDLGTGRGAFLKITNHNIGGPIFSGPHVTPFTCATPAATTTADG
                     APTNPSGLSGMPSTDGQCNIATEVRLFYRTSAACNPLTNPDPVPFPAQAPATACFKPY
                     NPASPPADVAMTTTDAGVTVPFIVRIERGTLNRGIFDIAVLFDPTKDDVKTGWKPVAP
                     QAAWNGKVLYSFGASSGQPRQQFHSEVAWADAGVTTNAVALGKGFLVAVNSMTDSLFN
                     DNRVLMTETLMMMKEKIIDSYGEVRYVMGNGCSGGSINQLTAASIFPGLLDGIQPTCT
                     YPDSETTAIEVSDCALLVNFYNSPAWTARLTAETITDQAAINNIKAAINGHLDQSGCH
                     AWVNSFTNLGRPGNYIPTVVLDNTSGVTGPDPRVTTKINNCRLPNSMVYDPVTNPDGV
                     RCTAADNAVAIFGKVKDTKRAQATADNVGIQYGLKAFLAGKISAEMFVVLNENVGGVD
                     GDDNFTSAAAGAHAPFARSVADAGALATAYRAGIVGDAAHWAKTPIIDLRGWDDKAIH
                     HVWRSFALRARLDAANGNHANHVMWRYPAALAAVQSIDPATSALTMQSFLMMDQWLTA
                     MKTDTKSTTQEARIAAARPAGAFDFCNKPLSPANLFSTSVVTDFSVCDSDPALVPHSS
                     PRQVAGGPVAENVLKCQLRPINRADYNPIGLTDDQFNRLKAVFPDGVCDFTRPGVGQQ
                     PAVGPLDFSAGPGGVPFADPPQQQAF"
     gene            complement(3596..>3942)
                     /locus_tag="VF516_11490"
     CDS             complement(3596..>3942)
                     /locus_tag="VF516_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108345.1"
                     /translation="DRGGSPAELPVEARRDAPGKATADRRRPAADPYQERVRARKAEA
                     DRCTAAHGDLPPGTSMKIVIGPDGRPRSVTLAPAAVDATPLGTCIKKVFRGVTFPRTD
                     HDYDLDVRLHPG"
BASE COUNT          542 a         1347 c         1427 g          626 t
ORIGIN      
        1 ctcagccccg gcgagatcct cgcgaccggc gccgaggccc gcgcgatcgc cggcggcgag
       61 cgccgcgcgg tgacctacgg cgacctgcgc agcgggatcg agcgccgcct gcgcaactcg
      121 ctgcaggacc tcgcgtggcg gatcgacgtc cgcgtgcggt ggagcgacct ggtgctgccg
      181 cccgagggcc gcggccgggt cgaggagttc atcgcgcgcc gcgcgttcgc ccacaaggtc
      241 tacggcgagt ggggcttcgg cgaccggctc gagcgcggca agggcagcat cgcgctgttc
      301 tccggcccgc ccggcaccgg caagaccatg ctcgccggcc tgatcgccca gggcctcggc
      361 ctcgacctct accaggtcga cctgtcgcag atccagtcgc gctggatcgg cgagaccgag
      421 aagcagctcg gccgcgtgtt cgacctcgcc gagcgcgccc acgccgtgct gctgttcgac
      481 gaggccgact cgctgctcgc caagcggacc gacgtcgaga cctccaacga tcgctacgcc
      541 aacgccaacg tcaactacct cttgcagcgg ctcgagcagt acaccggcgt cgtcgtgctc
      601 accaccaaca aggacgccgc gctcgacgag gccctccagc gccgcctgac cctccacctc
      661 cggctcgacg tccccgagat ccccgagcgc gagcagctgt ggcgctcgtt cctgccccag
      721 ggcgcgccgc gcgacgccga tctcgacgtc gcggcgctcg cccgcgagtt cgagctgacc
      781 ggcggcctga tcaagaacgt cgtggtccgc gccgcgttcc tcgccgcgcg cgaggaccaa
      841 aagctcggca ccggcctgct ccgccgcgcc gccacgctcg agctcgagga catgggccgc
      901 gtggtccgcg cggcctgacg gctagaacgc ctgctgctgc ggcgggtccg cgaacggcac
      961 gccgcccggg ccggccgaga agtcgagcgg gcccaccgcc ggctgctggc cgacgccggg
     1021 cctggtgaag tcgcacacgc cgtcggggaa caccgccttc agccggttga actggtcgtc
     1081 ggtcagcccg atcgggttgt agtcagcgcg attgatcggc cgcagctggc acttgagcac
     1141 gttctcggcg accgggccgc cggcgacctg gcgcggcgag ctgtgcggca cgagcgcggg
     1201 atcggaatca cacaccgaga agtcggtcac cacgctggta ctgaacagat tcgcggggct
     1261 gagcggcttg ttgcagaagt cgaaggcgcc ggccggccgg gccgccgcga tccgggcctc
     1321 ctgcgtggtg gacttggtgt cggtcttcat cgccgtgagc cactgatcca tcatcaggaa
     1381 tgactgcatc gtgagggcgg aggtcgccgg gtcgatggat tgcaccgccg cgagcgccgc
     1441 cggatagcgc cacatgacat ggttggcgtg gttgccgttg gcggcgtcga gccgggcgcg
     1501 cagcgcgaag ctgcgccaca cgtggtggat cgccttgtcg tcccagccgc gcaggtcgat
     1561 gatcggcgtc ttggcccagt gggcggcgtc gccgacgatg cccgcgcggt atgcggtcgc
     1621 cagcgcgccg gcgtcggcca cgctgcgcgc gaacggcgca tgggcgcccg ccgccgccga
     1681 cgtgaagttg tcgtcgccgt cgacgccacc gacgttctcg ttcaggacga cgaacatctc
     1741 ggcgctgatc ttgccggcca ggaacgcctt gaggccgtac tggatgccga cgttgtccgc
     1801 ggtcgcctgc gcgcgcttgg tgtccttcac cttgccgaag atcgcgaccg cgttgtccgc
     1861 cgcggtgcag cgcacgccgt ccgggttggt gaccgggtca tacaccatcg agttgggcag
     1921 cctgcaattg ttgatcttgg tggtcacccg cgggtcaggg ccggtcacac cggaggtgtt
     1981 gtcgagcacg acggtcggga tgtagttccc gggccggccg aggttggtga agctgttgac
     2041 ccaggcatgg cagccggact gatcgaggtg gccgttgatc gccgccttga tgttgttgat
     2101 cgcggcctgg tcggtgatgg tctcggcggt gagccgcgcc gtccaggccg gagagttgta
     2161 gaagttcacg agcagcgcgc agtcggatac ctcgattgcc gtggtctccg agtccggata
     2221 cgtgcaggtc ggctggatgc cgtccagcag cccggggaag atcgacgcgg cggtcagctg
     2281 gttgatcgat ccgccggagc agccattgcc catcacgtag cggacctcgc cgtagctgtc
     2341 gatgatcttc tccttcatca tcatcagggt ctcggtcatc agcacccggt tgtcgttgaa
     2401 cagcgagtcg gtcatgctgt tgaccgcgac caggaagccc ttgcccagcg cgacggcgtt
     2461 ggtcgtgacc ccggcgtccg cccatgccac ctcggagtgg aactgctggc gcggctgacc
     2521 gctcgacgcg ccgaacgagt agagcacctt gccgttccag gccgcctggg gcgcgaccgg
     2581 cttccagccg gtcttgacgt cgtccttggt cggatcgaac agcacggcga tgtcgaagat
     2641 cccgcggttg agcgttccgc gctcgatccg gacgatgaac ggcaccgtca cgccggcgtc
     2701 ggtggtggtc atcgccacgt ccgccggcgg cgacgccggg ttgtagggtt tgaagcacgc
     2761 agtggcaggg gcctgcgccg ggaacgggac cggatcgggg ttggtcagcg ggttgcacgc
     2821 tgcgctggtc cggtagaaca gccgcacttc ggtcgcgatg ttgcactgcc catcggtgct
     2881 gggcatgccc gacagcccgc tcggattggt cggcgcaccg tcggccgtgg tcgtagcggc
     2941 cggggtcgcg caggtgaacg gcgtgacgtg cgggcccgag aagatcggac cgccgatgtt
     3001 gtggttggtg atcttgagga acgcgccgcg gccggtcccg aggtcgaccg acagggtatt
     3061 ggcaccttcg gcgagcccgg tgaccaggcc gatgaggcga ccgtcggcgc gcgccgtgaa
     3121 ccggtcgctc acgtcctgga ccatgtccgg cttgccgggc gggcctaccg tgacgtgcag
     3181 cgtcgctgcc gtcgcaccgg gagggagcac gatctcgacc agcgcgtccc cgccgctgat
     3241 catgtcggcg cggttcgata gcgtcttgat ctgcggcacc ggctgcgacg gcggcgccgt
     3301 cgacccggtc gaggacgagt tgctgtcgca acccgacgcg gcgagcgcgg cggcggcgag
     3361 ccacagcgag cacggagatg gaacccgtag catcatcacg gtctccttgt gtcagtccgg
     3421 ggagcgcacc cgggcaagcc tcggttctga atgcggctga gtctccgccg ccggcgcgac
     3481 aggggtcaag cattcccggt gtcggagccg gtgcaattca cgctctgcca cggacctgct
     3541 cggcgcgctg agcgcggtgc gtcatttcgg gcccgagcgg caccctgccc cggcgttacc
     3601 cgggatgcag ccggacgtcg aggtcgtagt cgtgatcggt gcgcgggaac gtcacaccgc
     3661 ggaatacctt cttgatgcac gtgccgagcg gcgtggcgtc gacggcggcg ggcgcgagcg
     3721 tgacgctccg ggggcggccg tccggaccga tcacgatctt catcgaggtg ccgggcggca
     3781 gatcgccgtg tgcggcggtg cagcggtcgg cctcggcctt gcgggccctg acccgctcct
     3841 ggtacgggtc ggccgccggg cggcgccggt cggccgtcgc cttgccgggt gcatcgcgac
     3901 gggcctcgac cggcagctcc gccggcgagc cgccgcggtc cg
//
LOCUS       DASVOR010000582         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443607,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000582 DASVOR010000000
VERSION     DASVOR010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443607"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /locus_tag="VF516_11495"
     CDS             complement(<1..1548)
                     /locus_tag="VF516_11495"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX8108346.1"
                     /translation="MSRTGLRASLIWRDEVMEDLVLDKPRPITIGRTGRTTFTVPEIG
                     LPPKFAIVRPGKRGYLLTLGERMRGTICIDGKKQDVADLVRRDSAGGFSATPIGARDW
                     GVIDLDDSGDYKLFFQFVQVDDPPQFFTRPVLIAGGIGYLISSAALTLLWYLKGIELD
                     EAALRGAGIAALAMIGGGIGWSLIRQDGESQASLAFSVVLHAALLFMTYQLYESDNPF
                     TWPGPRSLTGNYLVTRLDKEEPPETKPPPTAGAQQQEAPAAATKEPPKKTATKGAEGK
                     AGGKGDTERARDPNAKDVPPAAPKVQFFTDTNKKVLDNIIDRNLTTSLSRFTGIKGDT
                     LTRGSLGFGPGAGTGVGAGEGTGTRTGSRKGGPGGGGNVEGDFVTGPGRIDTGTNRPG
                     GGTCKGAGCGTGPKPVQVAFAEPSGDFGGLTAEEIDRVVKARAGIFRACYQKELNHTP
                     GIGGKLLIHFKIGADGTVSAGNTSTASGTTLHNDAVEQCVKSNVNRLKFPAKGGIANV
                     NYPFVFTQ"
     gene            complement(1545..1847)
                     /locus_tag="VF516_11500"
     CDS             complement(1545..1847)
                     /locus_tag="VF516_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108347.1"
                     /translation="MCAFSLPAAAQPKGKAGAAKADARSGDKPGAAGATGATGSSDKK
                     PKEKTFDFNNLQLNGQLRTPQLLYFLERANEELERASLEKRSFIPHMVRSMEEEAL"
BASE COUNT          252 a          690 c          740 g          366 t
ORIGIN      
        1 ttgcgtgaac acgaacggat aattcacgtt ggcgatgccg cccttggccg gaaacttgag
       61 gcggttgacg ttggacttca cgcactgctc gaccgcgtcg ttgtgcagcg tggtgccgct
      121 cgccgtcgag gtgttgccgg cggagaccgt gccgtcggcg ccgatcttga agtggatcag
      181 cagcttgccg ccgatgccgg gcgtgtggtt gagctccttc tggtagcagg cgcggaagat
      241 gccggcgcgc gccttgacga cgcgatcgat ctcctcggcg gtgagcccgc cgaagtcgcc
      301 gctcggctcg gcgaacgcga cctggaccgg cttggggccg gtgccgcagc cggcgccctt
      361 gcaggtgccg ccgccggggc ggttggtgcc ggtgtcgatc ctgcccgggc cggtgacgaa
      421 gtcgccctcg acgttgccgc cgccgcccgg gccgcccttc ctgctgccgg tgcgcgtgcc
      481 ggtgccctca ccggcgccga cgccggtgcc ggcgcccggg ccgaagccga gcgagccgcg
      541 ggtcagcgtg tcgcccttga tgccggtgaa cctggacagg ctggtcgtca ggttgcgatc
      601 gatgatgttg tcgaggacct tcttgttggt gtcggtgaag aactggacct tgggcgccgc
      661 gggcggaacg tccttggcgt tgggatcgcg ggcgcgctcg gtgtcgccct tgccgccggc
      721 cttgccctcg gcgcccttgg tcgcggtctt cttcggcggc tccttggtcg ccgcggcggg
      781 cgcctcctgc tgctgcgcgc cggccgtcgg gggtggcttg gtctcgggcg gctcttcctt
      841 gtcgagccgg gtcaccaggt agttgccggt cagcgagcgc gggccgggcc acgtgaacgg
      901 gttgtcgctc tcgtagagct ggtaggtcat gaacagcagc gcggcgtgca gcacgaccga
      961 gaacgccagc gaggcctggg actcgccgtc ctggcggatc aggctccagc cgatgccgcc
     1021 gccgatcatc gcgagcgcgg cgatcccggc gccgcggagc gccgcctcgt cgagctcgat
     1081 gcccttcagg taccagagca gggtcagcgc ggcgctcgag atcaggtagc cgatgccgcc
     1141 ggcgatcagc accggccggg tgaagaactg cggcggatcg tcgacctgga cgaactggaa
     1201 gaacagcttg tagtcgccgg agtcgtcgag atcgatcacg ccccagtcgc gagcgccgat
     1261 cggcgtggcg gagaacccgc cggcgctgtc gcgccggacc aggtcggcga cgtcctgctt
     1321 cttgccgtcg atgcagatcg tgccgcgcat gcgctcgccg agggtgagca ggtagccgcg
     1381 tttgcccggc cggacgatcg cgaacttcgg cggcaggccg atctcgggga cggtgaacgt
     1441 ggtgcggccg gtccggccga tcgtgatcgg ccgcggcttg tcgagcacga ggtcctccat
     1501 gacctcgtcg cgccagatca gcgaggcccg caggccggtc cggctcacaa cgcttcttcc
     1561 tccatcgagc gcaccatgtg cgggatgaac gaccgcttct ccaggctcgc ccgctcgagc
     1621 tcctcgttgg cgcgctccag gaagtacaag agctgcggcg tgcgcagctg gccgttcagc
     1681 tggaggttgt tgaagtcgaa ggtcttctcc ttgggcttct tgtcactgga ccccgtcgcc
     1741 cccgtcgccc ccgccgcccc gggcttgtcg ccggaccggg cgtcggcctt cgcggcgcct
     1801 gccttgccct tcggctgggc cgctgcgggt agcgaaaacg cacacatcag cgccaggatc
     1861 gcggtcgcaa gcccccggcc gggggcggca agcctcagag tcatttcccg agctcctttc
     1921 gtttttcttc tgctgcctgg cgcttggcgt ggctcaccgg ggcctcttgc aggtatcgct
     1981 ccagggcggt ccggccgccg gtggcgtcgt tcatgtaatc gagctgcagg atggcgacgt
     2041 cgaacatc
//
LOCUS       DASVOR010000583         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_385173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000583 DASVOR010000000
VERSION     DASVOR010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VF516_11505"
     CDS             <1..1075
                     /locus_tag="VF516_11505"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX8108348.1"
                     /translation="LLAAARLAGRGEVAGLGPQPRKKLQSFVELIDGLGDVIANGASV
                     AETIIQVIERSGIRAKLEADQTTESRDRLDNLAELVTTASDFDDEVPPAPGSSMTGLA
                     AAGGGAGADGEARGENDRDPDQPGGDAEPGPPTLEDFLERIALSSPADQSGHEQVMLM
                     TIHIAKGLEWPIVFLTGMEDGLFPSLREREGVSEDAALEEERRLAYVAITRARERLVL
                     SQARTRRVWGEIRIQDPSRFLDDLPADSLAQPRRARISVPAPRIVDGDWAGRARRPRP
                     ARSRDELDQRTGYDDEPVFQVDGAGGFVIGAEVTHAALGAGRVVAVTGTGKDCRVVVD
                     FAAVGRKTVFAKFLEHGSGDRLN"
     gene            1212..1439
                     /locus_tag="VF516_11510"
     CDS             1212..1439
                     /locus_tag="VF516_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108349.1"
                     /translation="MRGIVLENDGRSGKVEVEVEVEVEVEVEVEVEVEVEVEVDGGVD
                     VLRRRQGPRWSQRRGQPQGGGPRSNALIDTS"
     gene            1538..2629
                     /locus_tag="VF516_11515"
     CDS             1538..2629
                     /locus_tag="VF516_11515"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8108350.1"
                     /translation="MGAESKDAPKPAPDPDPDVWIEKVVSVAGALGMNPVRVRWKLIR
                     WQEGRRRAQRRREQWVTHVRYQHKTCPTCGAVQDRDEKVCTACGAALTRRGVQVLQRI
                     GLAAPELISMSTLLALALLGVYLRAWIAAGGGLRSPGLEVLRAFGGHWRAGTAEEPWR
                     LVTAIFLHAGLWHLGFNLLAIASIGPRIEALYGRLTMLMLFVITGALANLGSDRMGLY
                     GVGIGASGGVMGLIGAAAGYGQRQGTAGGRALRNAMLQWSVYTFVFGFWVHADNWAHA
                     FGLVTGAVFGYAVRPAAWSHPRLLPVRVLGKLVGAAGAIAALAIIFTRMPPPPPEPRS
                     DRAAAATDADDVGASPATRATPASSGARR"
BASE COUNT          368 a          894 c         1082 g          387 t
ORIGIN      
        1 cctgctcgcg gcggcgcggc tcgccgggcg cggcgaggtc gccgggctgg ggccgcagcc
       61 gcgcaagaag ctgcagtcgt tcgtcgagct gatcgacggg ctcggcgacg tgatcgcgaa
      121 cggcgcgtcg gtcgccgaga ccatcatcca ggtgatcgag cgcagcggga tccgcgccaa
      181 gctcgaggcc gatcagacca ccgagtcgcg cgaccggctg gacaacctcg ccgagctggt
      241 gaccacggcg tcggacttcg acgacgaggt cccaccggcg cccggatcgt cgatgaccgg
      301 gctggcggcc gccggcggcg gcgcgggggc cgacggcgag gccaggggcg agaatgatcg
      361 cgatcccgat cagccgggcg gtgacgccga gccggggccg cccacgctcg aggacttcct
      421 ggagcggatc gcgctgtcgt cgccggccga tcagagcggg cacgagcagg tgatgctgat
      481 gacgatccac atcgccaagg gcctggagtg gccgatcgtg ttcctcaccg gcatggagga
      541 cgggctgttc ccgtcgctgc gcgagcgcga gggcgtcagc gaggacgccg cgctcgagga
      601 ggagcgccgg ctcgcctacg tcgcgatcac ccgcgcgcgc gagcggctcg tgctgtcgca
      661 ggcgcggacc cgccgggtgt ggggcgagat ccggatccag gatccgtcgc gcttcctcga
      721 cgatctgccg gcggattcgc tggcgcagcc gcgccgcgcc cggatcagcg tgcccgcgcc
      781 gcggatcgtg gatggagact gggccggccg ggcgcgccgg ccgcgtcccg cgcggtcgcg
      841 cgacgagctc gaccagcgga ccggctacga cgacgagccg gtgttccagg tcgatggcgc
      901 cggtggcttc gtgatcggcg ccgaggtcac ccacgccgcg ctcggcgcgg gccgggtcgt
      961 cgcggtgacc ggcaccggca aggactgccg ggtcgtggtc gacttcgcgg cggtcgggcg
     1021 caagacggtg ttcgccaagt tcctcgagca tggctcgggc gatcgcctga actgaagagc
     1081 tgcagatcaa aggagcggac cttcgtcccg cagacgttga gcttgacttc gacgtcggcg
     1141 caaggcgttg accttgaggg cgacgtagtc gttgagcccg tcgtcgacct tggacgtgaa
     1201 ccccggctcg tttgcgagga atcgtcctcg agaatgacgg gagatcgggc aaggtcgagg
     1261 tcgaggtcga ggtcgaggtc gaggtcgagg tcgaggtcga ggtcgaggtc gaggtcgagg
     1321 tcgaggtcga cggtggggtc gacgtcctac gtcgccgtca aggcccacga tggagtcaac
     1381 gacgaggtca acctcaaggt ggggggccca gatcgaacgc tttgatcgac acatcttgag
     1441 acggggctga cggacccgag cgatcgccgc gatttgccgg acgccgtggg ccgcgctggt
     1501 tggcagaccc cgggccgaag ctggatactc ggtggggatg ggtgccgagt cgaaggatgc
     1561 gccgaagccg gctccggatc cggatccgga tgtgtggatc gagaaggtcg tgagcgtcgc
     1621 gggggcgctc gggatgaacc cggtgcgcgt gcgctggaag ctgatccgct ggcaggaggg
     1681 ccggcggcgg gcgcagcgcc ggcgcgagca gtgggtcacg cacgtccgct accagcacaa
     1741 gacctgtccg acgtgcgggg cggtccagga ccgggacgag aaggtgtgca ccgcgtgcgg
     1801 cgccgcgctg acccgccgcg gggtccaggt gctgcagcgg atcggcctgg ccgcgcccga
     1861 gctgatctcg atgtcgacgc tgctggcgct ggcgctgctc ggcgtgtacc tccgcgcgtg
     1921 gatcgccgcg ggcggtgggc tccgatcgcc cgggctcgag gtgctccgcg cgttcggcgg
     1981 gcactggcgc gccggcaccg ccgaggagcc gtggcggctg gtcacggcga tcttcctgca
     2041 cgccggcctg tggcacctcg gcttcaacct gctcgcgatc gcctcgatcg ggccgcggat
     2101 cgaggcgctg tacggccggc tgacgatgct gatgctgttc gtgatcaccg gcgcgctcgc
     2161 caacctcggc agcgatcgga tggggctgta cggggtcggg atcggcgcgt cgggcggcgt
     2221 gatggggctg atcggcgccg ccgcggggta cggccagcgc cagggcacgg ccggcgggcg
     2281 cgcgctgcgc aacgcgatgc tgcagtggag cgtgtacacg ttcgtgttcg ggttctgggt
     2341 ccacgccgac aactgggcgc acgcgttcgg cctggtgacc ggcgcggtgt tcggctacgc
     2401 ggtgcggccg gcggcgtggt cgcatccccg gctcctgccg gtgcgcgtgc tcggcaagct
     2461 cgtcggggcg gccggcgcga tcgccgcgct ggcgatcatc ttcacgcgga tgccaccgcc
     2521 gccgccggag ccgcgatccg atcgggccgc tgcggccacc gacgccgatg acgtgggcgc
     2581 atcgcccgct actcgggcta ctccggcatc ttctggggca cgacggtgac gtcgaggcgc
     2641 gcgcggtcgc gccagatcgc gacgtggacc ggccggccgg ccggggcctg agcgaccacc
     2701 cagggcagca accgcggatc ggcgtcgcga t
//
LOCUS       DASVOR010000584         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_5580,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000584 DASVOR010000000
VERSION     DASVOR010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5580"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VF516_11520"
     CDS             <1..531
                     /locus_tag="VF516_11520"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8108351.1"
                     /translation="LFLADAHLPDYITMHRQYVDAGLCHKVVSYGARGSEKAAIEALG
                     KDNTEYVLSGTWWSPLLANTNPLAKKFVEAFKAKYGGRTPEWFQALGYESVRALVEAI
                     HQAGSLDREAVRAKLAALKMESLVPGGTLDFPQDKGYQIQAPFVVQQNTPDGAPIIFP
                     QSIATAAGIAPNPRCK"
     gene            533..1408
                     /locus_tag="VF516_11525"
     CDS             533..1408
                     /locus_tag="VF516_11525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8108352.1"
                     /translation="MARFADLVVSAVLLAGLYATMAYGLGLIYGVLRVVNLAHGGVIM
                     AGAYVGWIVFDRFGVDPYLAIPVVGVAGFALGVAMYRLLVRRLPRGAAGGPASLLLLF
                     GVWLVLRNIAYLVFTGNDRTIRTAYSSSSLAIAGTHVSLTRLVVLAIGLVIAVGLHLL
                     LHRTLLGRAIRAVAQNADSCTLVGIDVERIYLVTFGIGSALAAVAGVLLATVFSFNPA
                     SGSSELLKSFVVVVLGGLGSMSGVVLAALVVAAAEVFAVLILPSYLTTAVGFLLLVLV
                     LVLRPGGLFGHRVLA"
     gene            1405..2379
                     /locus_tag="VF516_11530"
     CDS             1405..2379
                     /locus_tag="VF516_11530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8108353.1"
                     /translation="MTAPPLIPRATARAFVAVCAILAALPAADLSLYTHNLLTRAYLL
                     IAAALAWNWMGGLVGQISFGHAAMFGVGGFVAGRLMLDLHLPPQLCWLAGAAAAGVFA
                     LGAHPLLRLRGPYFSIATIGVGEASRVVFTYWEKFTGGTTGLSLPIDADFQYQLYWWT
                     LGLAAAVAAASVVIRRSSFGLKLLAIKADVDAASDVGISATLYQDLIFALSGAVVGLC
                     GAISASYYSFIEPNDTFAFERSIAFVLMAVIGGIGTALGPALGAAVFVLVDTYLLASY
                     PELHLGLYGVLLIAIILFEPLGLTGLGRRLGRGMMRRVRRPAPAGERA"
     gene            2382..>3002
                     /locus_tag="VF516_11535"
     CDS             2382..>3002
                     /locus_tag="VF516_11535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8108354.1"
                     /translation="MALLQTRGLTRRFGGLTALDRVDLALGAGEILGVIGPNGAGKTT
                     LFSLIAGSLRPSAGEILLDGRPVTRLAAHRRVRAGIVRTHQIVRPFANLTVFENVLVA
                     AMHAHGGAAGHARDRAADVLELVGLADRYDQSPGSLTLAGRTRLELARALSTGPRVLL
                     LDEVAGGLVEHETAALVELVKQVRASGVSIVWIEHIVHALIAAVDRL"
BASE COUNT          336 a         1048 c         1112 g          506 t
ORIGIN      
        1 ctgttcctcg ccgacgcgca cctgccggac tacatcacga tgcaccgcca gtacgtcgac
       61 gcggggctgt gccacaaggt ggtgagctac ggcgcgcgcg gctcggagaa ggccgcgatc
      121 gaggcgctcg gcaaggacaa caccgagtac gtgctgtccg ggacgtggtg gagcccgctc
      181 ctggccaaca ccaacccgct ggccaagaag ttcgtcgagg cgttcaaggc caagtacggc
      241 ggccggacgc cggagtggtt ccaggcgctg ggctacgagt cggtgcgcgc gctggtcgag
      301 gcgatccacc aggccggctc gctcgatcgc gaggcggtgc gcgccaagct cgccgcactg
      361 aagatggagt cgctggtgcc gggcgggacg ctcgacttcc cgcaggacaa gggctaccag
      421 atccaggcgc cgttcgtggt ccagcagaac acgcccgacg gcgcgccgat catcttcccg
      481 cagagcatcg ccacggcggc cggcatcgcg cccaatccgc gctgcaagtg agatggcgcg
      541 gttcgccgac ctcgtcgtct ccgcggtgct gctcgccggg ctgtacgcca cgatggcgta
      601 tggcctcggg ctgatctacg gcgtcctgcg cgtggtcaac ctcgcgcacg gcggcgtgat
      661 catggccggg gcgtacgtcg gctggatcgt gttcgatcgg ttcggcgtcg acccgtacct
      721 cgcgatcccc gtggtggggg tggccggctt cgcgctcggc gtggcgatgt accggctgct
      781 ggtccggcgg ctgccgcgcg gcgcggccgg cggcccggcg tcgctgctgc tgctgttcgg
      841 ggtctggctg gtgctgcgca acatcgcgta cctggtgttc accggcaacg atcggacgat
      901 ccgcaccgcg tactcgtcga gctcgctggc gatcgccggc acccacgtgt cgctgacccg
      961 gctggtcgtg ctcgcgatcg gcctggtgat cgcggtcggc ctccacctgc tgctccaccg
     1021 cacgctgctc gggcgcgcga tccgcgcggt cgcgcagaac gccgacagct gcacgctggt
     1081 cggcatcgac gtcgagcgga tctacctggt gacgttcggc atcggcagcg cgctggccgc
     1141 ggtcgccggc gtgctgctcg cgacggtgtt ctcgttcaac ccggcgtcgg ggtcgtccga
     1201 gctgctcaag tcgttcgtcg tggtcgtgct cggcgggctg ggcagcatgt ccggcgtggt
     1261 gctggcggcg ctggtggtcg cggcggccga ggtgttcgcg gtgctgatcc tgccgtcgta
     1321 cctgaccacg gcggtcgggt tcctgctgct ggtgctggtg ctggtgctgc ggccgggcgg
     1381 cttgttcggt caccgggtcc tggcatgacc gcgccgccgc tgatcccgcg cgcgacggcc
     1441 cgggcgttcg tcgcggtgtg cgcgatcctc gccgcgctgc cggcggccga tctgtcgctg
     1501 tacacccaca acctgttgac ccgcgcgtac ctcctgatcg ccgcggcgct ggcgtggaac
     1561 tggatgggcg ggttggtcgg gcagatcagc ttcggccacg cggcgatgtt cggggtcggc
     1621 gggttcgtcg ccgggcggct gatgctcgac ctccacctgc cgccgcagct gtgctggctc
     1681 gccggcgcgg cggccgccgg ggtgttcgcg ctgggcgccc atccgctgct ccggctgcgc
     1741 gggccgtact tctcgatcgc gacgatcggt gtcggcgagg ccagccgggt ggtgttcacc
     1801 tactgggaga agttcaccgg cggcacgacc gggctgtcgc tgccgatcga tgcggacttc
     1861 cagtaccagc tgtactggtg gacgctgggg ctggcggccg cggtcgccgc ggcgtcggtc
     1921 gtgatccggc ggtcgagctt cgggctcaag ctgctcgcga tcaaggccga cgtcgacgcc
     1981 gcctccgacg tcgggatctc ggccacgctg taccaggacc tgatcttcgc gctgtccggc
     2041 gcggtggtcg ggctgtgcgg cgcgatcagc gcgtcgtact acagcttcat cgagcccaac
     2101 gacacgttcg cgttcgagcg ctcgatcgcg ttcgtgctga tggcggtgat cggtgggatc
     2161 gggaccgccc tggggccggc gctcggcgcc gcggtgttcg tgctggtcga tacctacctg
     2221 ctcgcgtcgt accccgagct ccacctcggg ctctacggcg tgctgctgat cgcgatcatc
     2281 ctgttcgagc cgctcgggct gaccggcctg ggccgccgcc tgggccgcgg gatgatgcgc
     2341 cgcgtgcggc ggccggcgcc ggcgggggag cgggcgtagc catggcgctg ctccagaccc
     2401 gcgggctgac ccgtcgattc ggcggcctca ccgcgctcga ccgcgtcgac ctcgcgctcg
     2461 gcgccggcga gatcctcggc gtgatcggcc ccaacggcgc cggcaagacc acgctgttca
     2521 gcctgatcgc cggctcgctg cggccgagcg ccggtgagat cctgctcgac ggccggccgg
     2581 tgacccggct cgcggcgcac cgccgggttc gcgccggcat cgtccgcacc caccagatcg
     2641 tgcggccgtt cgccaacctg acggtgttcg agaacgtgct ggtcgccgcg atgcacgccc
     2701 acgggggcgc cgccggccac gcgcgcgacc gcgccgccga cgtgctggag ctggtcggcc
     2761 tcgccgatcg ctacgaccag tcgcccggct cgctgaccct ggccgggcgc acgcggctcg
     2821 agctcgcgcg cgcgctgtcg accgggccgc gcgtgctgct gctcgacgag gtggccggcg
     2881 gcctggtcga gcatgaaacc gccgcgcttg tggaactggt caaacaggta cgcgccagcg
     2941 gggtgtcgat cgtctggatc gagcacatcg tgcacgcgct gatcgccgcg gtggatcggc
     3001 tt
//
LOCUS       DASVOR010000585         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_413981,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000585 DASVOR010000000
VERSION     DASVOR010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413981"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..398
                     /locus_tag="VF516_11540"
     CDS             <1..398
                     /locus_tag="VF516_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108355.1"
                     /translation="CLVYEQAGYAELRKGLPKLGKRVIGARGEARVVEVDVLRQRVRI
                     SYAPGDTEVVAAAEVRPMFPSGNPPHARGADPGASDDADAHDAHDAHDGSDDAIDLPD
                     GLADALASTLPELPDDDPALPDDDPSSAP"
     gene            395..>1916
                     /gene="metG"
                     /locus_tag="VF516_11545"
     CDS             395..>1916
                     /gene="metG"
                     /locus_tag="VF516_11545"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEX8108356.1"
                     /translation="MTQTFYITTPIYYVNDVPHLGHAYTTLVADSLARFHRMRGDDTR
                     FLTGTDEHGQKVEEAAGKRGLTPRQLVDQVAPRFDETWKTLGITGYRFIRTTSDLHRR
                     VVQNLWRRIRAAHPDDLYLASYQGWYCVGCEAFYTESQLQRDGDTWVCEVHKKPVAWL
                     DKERSWFFRLSRYAEPLLRHIEAHPEFIRPEAYRNEIVAFLRGGLRDLSVSRTSFAWG
                     IPVPEDDPEGLQHVIYVWMDALVNYLTDLCPDSGDITGPDVEHYWPATTHLIGKDILR
                     FHSVYWPAFLMAANLPLPRGIFAHGWWTVRGQKISKSMPATRIDPIKLADALGDGSPL
                     GRALGIDAMRYYLLREMPLGNDGDFTFESLFGRFNAELANDLGNLINRSLTLIVKLSA
                     QTPPARDDALYRAGENPYWQLEVHAAEACSHAAAQFDAFAPSRALEAVWRFIAMANRF
                     IDQTQPWAMVKSRDPALGHALWCLQSSLWLIARLIAPVLPATAGALRGWIGDGDAAM"
BASE COUNT          287 a          733 c          636 g          260 t
ORIGIN      
        1 gctgcctggt ctacgagcag gccggctacg ccgagctgcg caagggcctg cccaagctcg
       61 gcaagcgcgt gatcggcgcg cgcggcgagg cccgcgtcgt cgaggtcgac gtgctgcgcc
      121 agcgcgtccg gatctcgtac gccccgggcg acaccgaggt cgtcgccgcg gccgaggtcc
      181 gcccgatgtt cccgtcgggc aacccgccgc acgcgcgggg cgccgacccg ggggcgagcg
      241 acgacgccga cgcccacgac gcccacgacg ctcacgacgg cagcgacgac gcgatcgatc
      301 tccccgacgg gctcgcggat gcgctggcgt ccacgctccc cgagctcccc gacgacgacc
      361 ctgccctgcc cgacgacgat ccgagctccg cgccatgacc cagaccttct acatcacgac
      421 gccgatctac tacgtcaacg acgtcccaca cctcggccac gcctacacca cgctggtcgc
      481 ggactcgctc gcccggttcc accgcatgcg cggcgacgac acccggttcc tgaccggcac
      541 cgacgagcac ggccagaagg tcgaggaggc cgccggcaag cgcggcctga ccccgcggca
      601 gctggtcgac caggtcgcgc cgcggttcga cgagacctgg aagacgctcg gcatcaccgg
      661 ctaccgcttc atccgcacca cctcggatct ccaccggcgc gtggtccaga acctgtggcg
      721 ccggatccgc gcggcccacc cggacgacct ctacctcgcg tcctaccagg gctggtactg
      781 cgtcggctgc gaggcgttct acaccgagag ccagctgcag cgggacggcg acacctgggt
      841 gtgcgaggtc cacaagaagc cggtggcgtg gctcgacaag gagcgcagct ggttcttccg
      901 gctgtcgagg tacgccgagc cgctgctccg ccacatcgag gcccaccccg agttcatccg
      961 gcccgaggcc taccgcaacg agatcgtcgc gttcctgcgc ggcggcctgc gcgacctgtc
     1021 ggtgtcgcgc accagcttcg cgtggggcat cccggtcccc gaggacgacc ccgagggcct
     1081 ccagcacgtg atctacgtgt ggatggacgc gctggtcaac tacctgaccg acctgtgccc
     1141 ggacagcggc gacatcaccg gccccgacgt cgagcactac tggccggcga ccacccacct
     1201 gatcggcaag gacatcctgc ggttccacag cgtgtactgg ccggcgttcc tgatggcggc
     1261 gaacctgccg ctgccgcgcg ggatcttcgc ccacggctgg tggacggtgc gcggccagaa
     1321 gatctccaag tcgatgccgg cgacccggat cgatccgatc aagctcgccg acgcgctcgg
     1381 cgacggctcc ccgctgggcc gcgcgctcgg catcgacgcc atgcgctact acctcttgcg
     1441 cgagatgccg ctcggcaacg acggcgactt cacgttcgag tcgctgttcg gccggttcaa
     1501 cgccgagctc gccaacgacc tcggcaacct gatcaaccgc agcctgacgc tgatcgtcaa
     1561 gctgtcggcg cagaccccgc cggcgcgcga cgacgcgctg taccgcgccg gcgagaaccc
     1621 gtactggcag ctcgaggtcc acgccgccga ggcgtgcagc cacgccgccg cgcagttcga
     1681 cgcgttcgcg ccgtcgcgcg cgctcgaggc ggtctggcgg ttcatcgcga tggccaaccg
     1741 gttcatcgat cagacccagc cgtgggcgat ggtgaagtcc agggacccgg cgctcggcca
     1801 cgcgctgtgg tgcctgcagt cgagcctgtg gctgatcgcg cggctgatcg cgccggtctt
     1861 gcccgcgacc gccggcgcgc tgcgcggctg gatcggcgac ggcgacgccg cgatgg
//
LOCUS       DASVOR010000586         2520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_210028,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000586 DASVOR010000000
VERSION     DASVOR010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210028"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VF516_11550"
     CDS             complement(<1..277)
                     /locus_tag="VF516_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108357.1"
                     /translation="MPILQPDELRTSASEPTDDDSPDLDWTLALAGGEGVRLSEYVQR
                     RFGRRIPKQYCCLLGERSMLEHTLERLNKLTPPSRTLTVIGTDHGEIA"
     gene            complement(618..2027)
                     /locus_tag="VF516_11555"
     CDS             complement(618..2027)
                     /locus_tag="VF516_11555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8108358.1"
                     /translation="MAVDDLRSGTWVTYVGGRATSARLRRCRVEVVTGADAGLVRDIE
                     SSVIRIGARRGNDVQLTDSKVSGLHCEIRLDDRGYRLRDLDSTNGTYVSQLRVNDVYI
                     QPGTHIALGGTRLRFEPLGDSVEIELSDTDRFGSMIGRSVKMREMFARLEKLARTDTT
                     VLVTGETGVGKELVAEALHDHSPRAKGPFVVLDCGSIPPNLIESELFGHERGAFTGAT
                     ASYAGAFERAHGGTVFLDEIGELPLAMQPKLLRVLERKEVRRVGASKPIEVDLRVVAA
                     TNRDLGVEVNRGRFREDLYYRLAVARVHVPPLRERKDDLPLLIEHILATTPNGETASI
                     AQETIDLMMKHDWPGNVRELRNVIERAVLLAETPEDEDALRRTPAAVPRGEPSITATP
                     SHTATSADAAMTVPVDVGIPFKLAKQNVISEFEKRYISRLLAQHDGNISAAARAAGID
                     RMSIHKMLHRLGLSNPGRE"
     gene            2190..>2520
                     /locus_tag="VF516_11560"
     CDS             2190..>2520
                     /locus_tag="VF516_11560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108359.1"
                     /translation="MSAIQSLLRVMTLRDAEAIILEADKVPSLRRRGQIEKLAMPALD
                     PQLLADFATPLLAGRSLDDGPASVAFHEPGGAHYQVTLEKLATGLRVVVRPGKPPAAA
                     APAAPAPP"
BASE COUNT          336 a          896 c          891 g          397 t
ORIGIN      
        1 ccgcgatctc gccgtgatcg gtgccgatca cggtgagcgt gcgcgacggc ggggtcagct
       61 tgttgagccg ctccagcgtg tgctcgagca tcgagcgctc gccgagcagg cagcagtact
      121 gcttggggat ccgacggccg aacctgcgct ggacgtactc ggacagccgg acgccctcgc
      181 cgccggcgag cgccagcgtc cagtcgaggt ccggggaatc gtcgtcggtc ggttcactcg
      241 cagacgttct cagctcgtct ggctgaagga tcggcatctc cgctcggcac tgcgatgttc
      301 gtgccgagcg cggaccgctg ccggcccacc gtgggggttg gtgccgcacg cgagcttacc
      361 gcgcctgcat gggccgaccg cgtaccgcga gcgagcggtc gcgcgcggtg tctcgccagc
      421 gcgcgtgaac ggtttgcact ctccgggttg tcgagcgatc ctcgagatct ggattcccac
      481 ccgcgccgcg gccgatcgat cgcgcgcgct ggtgcatccg cgctcagcct cgtgcgacgg
      541 cggcggcgat tccccgatgc gccggccgcc accgccgccg gaatccgatc gaaatcgaga
      601 tcgcgatcgc gaccgggtta ttcccgaccg gggttggaca gcccgaggcg atgcagcatc
      661 ttgtggatcg acatccggtc gatccccgcg gcgcgggcgg cggccgagat gttgccgtcg
      721 tgctgggcga gcagccgcga gatgtagcgc ttctcgaact cgctgatcac gttctgcttg
      781 gcgagcttga acgggatccc gacgtcgacc ggcacggtca tcgccgcgtc ggcgctggtc
      841 gcggtgtgcg acggcgtggc ggtgatcgac ggctcgccgc ggggcaccgc cgccggcgtt
      901 cggcgcagcg cgtcctcgtc ctcgggagtc tcggcgagca gcacggcgcg ctcgatcacg
      961 ttgcgcagct cgcggacgtt gcccggccag tcgtgcttca tcatcaggtc gatcgtctcc
     1021 tgggcgatcg acgcggtctc gccgttgggc gtggtcgcga ggatgtgctc gatcagcagc
     1081 ggcaggtcgt ccttgcgctc gcgcagcggc gggacgtgaa cgcgcgcgac cgcgagccgg
     1141 tagtacaggt cctcgcggaa ccggccgcgg ttgacctcga cgccgagatc gcggttggtc
     1201 gcggcgacca cgcgcagatc gacctcgatc ggcttgctcg ccccgacccg gcggacctcc
     1261 ttgcgctcga gcacgcgcag cagcttgggc tgcatcgcga gcggcagctc gccgatctcg
     1321 tcgaggaata ccgtgccgcc gtgggcgcgc tcgaacgcgc cggcgtacga cgcggtcgcg
     1381 ccggtgaacg cgccgcgctc gtggccgaac agctcgctct cgatcaggtt gggcgggatc
     1441 gagccgcagt cgagcacgac gaacggcccc ttggcgcgcg gcgagtggtc atggagcgcc
     1501 tcggcgacca gctccttgcc gacgccggtc tcgccggtca ccagcaccgt ggtgtcggtg
     1561 cgcgcgagct tctccagccg tgcgaacatc tcgcgcatct tgaccgagcg gccgatcatc
     1621 gagccgaacc ggtcggtgtc ggacagctcg atctccaccg agtcgccgag cggctcgaac
     1681 cgcagccggg tcccgccgag cgcgatgtgg gtgccgggct ggatgtagac gtcgttgacc
     1741 cggagctgcg acacgtaggt cccgttggtc gagtcgaggt cgcgcaggcg gtagccgcgg
     1801 tcgtcgagcc ggatctcgca gtgcagcccc gacaccttcg agtcggtgag ctggacatcg
     1861 ttgccgcgcc gcgccccgat ccggatcacc gacgattcga tatcgcggac cagcccggca
     1921 tcggccccgg tcacgacctc gacccggcag cggcgcagtc gcgcggacgt ggcccggccc
     1981 ccaacgtagg tgacccatgt ccccgagcgc aggtcgtcga ccgccatgta gcaggcacgt
     2041 taccacagcg cgggagtcgt agatttctgg tttgagtcac gctggcgcgc cgcggcgccg
     2101 gcgctgccaa tctgactatc catgcgcgat cgagcaccgg tgtttcgcgc gattgcgggt
     2161 ggcgtggtgc ttgctgatgc ttccccacga tgtccgccat ccagtccctc ctgcgggtga
     2221 tgacgctgcg cgacgccgag gcgatcatcc tggaagccga caaggtcccg agcctgcggc
     2281 gccgcggcca gatcgagaag ctcgcgatgc ccgcgctcga cccgcagctg ctcgcggact
     2341 tcgccacgcc cctgctcgcc gggcgctcgc tcgacgacgg ccccgcgtcg gtggcgttcc
     2401 acgagcccgg cggcgcgcac taccaggtga cgctcgagaa gctcgccacc gggctgcgcg
     2461 tcgtcgtgcg ccccggcaag ccaccggcgg ccgccgcacc cgcggcgccc gccccaccgg
//
LOCUS       DASVOR010000587         3373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443668,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000587 DASVOR010000000
VERSION     DASVOR010000587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443668"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1001)
                     /gene="trpD"
                     /locus_tag="VF516_11565"
     CDS             complement(<1..1001)
                     /gene="trpD"
                     /locus_tag="VF516_11565"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEX8108360.1"
                     /translation="MIVEAINRALARRDLSRAEMAEVVGQIMDGAATPAQIGGLLIAL
                     RAKGESVDEIVGAATAMRSRALPLACPRHDRSIDTCGTGGDGSGTFNVSTLAAIVVAA
                     CGGVVAKHGNRALSSRCGSADVLEVLGIPVDVEPAAITRAIEVAGIGFAFAPKFHAAT
                     RHASGPRKELGTRTIFNLLGPLTNPAQVRHQVVGVFARTWCEPVARALGALGVRRAAV
                     VHGDGGLDEVAVRGNTFVAIWNEGEVTTRTLTPAAFGCDEADPEGLAGGDAPHNAAIM
                     RRVLAGREIGRGERRGRRQARQPRAVVALWLGRRARGTRDRDLRRAGRGDPRDRGRR"
     gene            complement(998..1633)
                     /locus_tag="VF516_11570"
     CDS             complement(998..1633)
                     /locus_tag="VF516_11570"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEX8108361.1"
                     /translation="MKVVLIDNYDSFTYNLAQYLGELGAEVAVERNDAITLDASAQRA
                     PDAIVISPGPCTPNEAGISMDVVRRFAGVTPILGVCLGHQCIGQVMGGRIVRAPRVMH
                     GKTSKIFHDERGLYAGVENPFVATRYHSLVIAPDSMPEALEVTAKTWEDEIMGVRGRR
                     TAAALEGVQFHPESIMTAVGKQLLANFLRMAREPHPAAEVGAGAGAGMGAP"
     gene            complement(1630..3192)
                     /locus_tag="VF516_11575"
     CDS             complement(1630..3192)
                     /locus_tag="VF516_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEX8108362.1"
                     /translation="MYTPSREEFIAAALRGNLIPVYRELLADGDTPVSAYAALGAGEH
                     SFILESVIGGATWAAYSFVGVAPRAVVRWHNSTARVTWYDVDAGGPPRTAEWQTPDPT
                     AALAEVLGEMRPVDVAGLPRFWGGAVGWIAYDCVRAFEDLPARARPGPRMEAGQLCWT
                     AAVDLPPLCMVVTDTLLIFDNLRQTLKVVATPYVARPERAEVAYDRACARIDAVIARL
                     RAPRTPLPALTPPRPNELPGAVPPSSFSKPEYVAAVDRVKEYILAGDAFQVVLSQRFA
                     EPAAGARPLDVYRALRVINPSPYMFHLEFPEARVTGASPETLVRLSDGRVELRPIAGT
                     KPRGATAAEDDRLAAELLADPKERAEHLMLIDLGRNDVGRVARVGSVHVVEQMVIEKF
                     SHVMHLTSHVVGTLAAGKTWVDVLRAAFPAGTLSGAPKIRAMEIIDELEPHQRGIYGG
                     AVGYVSYTGNLDLAIAIRTLVSCGDTIYVQAGGGLVADSVPETEYQETVNKARAVLRA
                     VAMARTAERTAP"
BASE COUNT          447 a         1263 c         1220 g          443 t
ORIGIN      
        1 tcccggcgac ctcgatcgcg cgggtcaccg cggccggcgc gacggagatc gcgatcccga
       61 gtgcctcgag cacgtcggcc gagccacagc gcgacgacag cgcgcggttg ccgtgcttgg
      121 cgacgacccc gccgctcgcc gcgcccgatc tcgcgcccgg cgagcacccg gcgcatgatc
      181 gccgcgttgt ggggagcgtc gccgccggcg agcccctccg ggtcggcctc gtcgcagccg
      241 aacgccgccg gggtcagcgt ccgggtcgtg acctcgccct cgttccagat cgcgacgaag
      301 gtattgccgc gcaccgcgac ctcgtcgaga ccgccgtcgc cgtgcaccac cgcggcgcgc
      361 cggacgccga gcgcaccgag cgcgcgggcc accggctcgc accacgtgcg cgcgaacacg
      421 ccgacgacct ggtgacggac ctgcgccggg ttggtcagcg ggccgagcag gttgaagatc
      481 gtgcgggtgc cgagctcctt gcgcggcccc gaggcatggc gggtcgcggc gtggaacttg
      541 ggcgcgaacg caaagccgat gccggcgacc tcgatcgccc gggtgatcgc cgccggctcg
      601 acgtcgaccg ggatgccgag cacctcgagc acgtcggccg agccgcagcg cgacgacagc
      661 gcgcggttgc cgtgcttggc gaccacgccg ccgcacgcgg cgaccacgat cgccgccagg
      721 gtcgagacgt tgaacgtgcc gctgccgtcg ccgccggtgc cgcaggtgtc gatcgagcga
      781 tcgtggcgcg gacaggccag cggcagcgcc cggctgcgca tcgcggtggc ggcgccgacg
      841 atctcgtcga ccgactcgcc cttggcgcgc agcgcgatca agaggccccc gatctgcgcg
      901 ggagtcgcgg cgccgtccat gatctggccg acgacctcgg ccatctcggc gcgcgacagg
      961 tcgcggcggg cgagcgcgcg gttgatcgcc tcgacgatca tggcgccccc atcccggccc
     1021 cggccccagc cccgacctcg gccgccgggt gcggctcgcg cgccatgcgc aggaagtttg
     1081 cgagcagctg cttgcctacc gcggtcatga tcgactccgg gtggaactgt acgccttcca
     1141 gcgcggccgc ggtgcgccga ccgcgcacgc ccatgatctc gtcctcccag gtcttggccg
     1201 tgacctcgag ggcttcgggc atcgagtccg gcgcgatcac cagcgagtgg tagcgggtcg
     1261 cgacgaacgg gttctcgacc ccggcgtaca ggccgcgctc gtcgtggaag atcttcgagg
     1321 tcttgccgtg catcacccgc ggcgcccgga cgatccggcc gcccatcacc tggccgatgc
     1381 actggtggcc gaggcagacc cccaggatcg gcgtcacgcc ggcgaaccgg cggaccacgt
     1441 ccatcgagat cccggcctcg ttcggcgtgc acggcccggg cgagatcacg atcgcgtcgg
     1501 gcgcccgctg ggcgctcgcg tcgagcgtga tcgcgtcgtt gcgctcgacc gcgacctcgg
     1561 cgccgagctc gccgaggtac tgcgcgaggt tgtaggtgaa cgagtcgtag ttgtcgatca
     1621 acaccacctt catggcgccg tccgttccgc ggtgcgcgcc atcgccaccg cgcgcagcac
     1681 cgcgcgcgcc ttgttgaccg tctcctggta ctcggtctcc ggcaccgagt cggcgaccag
     1741 cccgccgccg gcctggacgt agatggtgtc gccgcacgac accagggtgc ggatcgcgat
     1801 cgcgaggtcg aggttgccgg tgtagctgac gtagccgacc gcgccgccgt agatcccgcg
     1861 ctgatggggc tcgagctcgt cgatgatctc catcgcgcgg atcttcggcg cgccggacag
     1921 cgtgcccgcc gggaacgccg cgcgcagcac gtcgacccag gtcttgccgg ccgcgagcgt
     1981 cccgaccacg tgcgaggtca ggtgcatcac gtgggagaac ttctcgatca ccatctgctc
     2041 gacgacgtgg accgagccga cccgcgcgac ccggccgacg tcgttgcggc cgaggtcgat
     2101 cagcatcagg tgctcggcgc gctccttggg atcggcgagc agctcggcgg cgagccggtc
     2161 gtcctcggcc gcggtcgcgc cgcgcggctt ggtcccggcg atcggccgga gctcgacccg
     2221 gccgtcgctc agccggacca gggtctcggg cgacgcgccg gtgacccggg cctcggggaa
     2281 ctcgaggtgg aacatgtacg gcgacgggtt gatcacgcgc agcgcgcggt agacgtcgag
     2341 cggccgcgcg ccggccgccg gctcggcgaa ccgctgcgac agcacgacct ggaacgcgtc
     2401 gccggcgagg atgtactcct tgacccggtc gaccgcggcg acgtactcgg gcttgctgaa
     2461 cgacgacggc gggaccgccc ccggcagctc gttcggccgc ggcggggtca gcgctggcag
     2521 cggcgtgcgc ggcgcgcgca gccgggcgat gaccgcgtcg atccgcgcgc acgcccggtc
     2581 ataggcgacc tcggcgcgct cgggccgcgc gacgtagggc gtcgccacca ccttcaacgt
     2641 ctggcgcagg ttgtcgaaga tcagcagggt gtcggtcacc accatgcaca gcggcggcag
     2701 gtctaccgcg gcggtccagc aaagctggcc cgcctccatc ctcggccccg ggcgcgcccg
     2761 cgccggcagg tcctcgaacg cgcggacgca gtcgtacgcg atccagccga ccgcgccgcc
     2821 ccagaaccgc ggcagcccgg cgacgtcgac cgggcgcatc tcgcccagca cctcggcgag
     2881 cgcggcggtc ggatccgggg tctgccactc ggcggtgcgc ggcggcccgc cggcatcgac
     2941 gtcgtaccag gtcacgcgcg cggtgctgtt gtgccagcgc acgacggcgc gcggcgccac
     3001 cccgacgaac gagtacgccg cccaggtcgc gccgccgatc accgactcga gaatgaacga
     3061 gtgctcgccg gcacccagcg ccgcgtacgc ggacaccggg gtgtcgccgt cggccagcag
     3121 ctcgcggtag accgggatga ggttccccct cagcgcggcc gcgatgaact cctcccgcga
     3181 aggcgtgtac atcccgtccg cggttatacc cgtggcccca cggcgtggcc atcgcggcat
     3241 ggcccgggcg cggatcgcgt tcaccggtcg ccgagcctcc cgctgcggca tgggaagccg
     3301 atcgaccctg cgcgacggga tcgcgggtgc cggcgcggcg gccgcgcgat cgtgcgcggt
     3361 atgcctgaat ttg
//
LOCUS       DASVOR010000588         8763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_326975,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000588 DASVOR010000000
VERSION     DASVOR010000588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8763
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326975"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="VF516_11580"
     CDS             complement(<1..1424)
                     /locus_tag="VF516_11580"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8108363.1"
                     /translation="MSLFRLLERHLARGADRPLLVTAGGTLSSGDVARESAKLAAWLR
                     AAGTARGDRVVIDLKTGPDAVAALFGAARVGAIAVAASPQWTPQQIEHVIANSGARVL
                     ITGELRVQQLAGRTPPHVLVRGRGPAHQGTAWDTLVEAWDDAVDGCDEPAILLYTSGS
                     TGRPKGVVHSHRNLEDFARIVAGYLGNTADDRLLWLLGWNFGYGLSQLLTMCEAGGRL
                     IVPASVLAADVVTAHADHGATALAQVPFGWDQLVSFLEHTGRQLTGLRYVTNAGDRPS
                     PALIERMRRALPGAQIVLMYGQTECFRTTYLPAAQLDAKLGAMGYPIPEVEVRVVAPD
                     GTPCPPGVTGELHHAGPLVAAGYWEDAQATAAKFYRDGERRVLRTGDLVRRDDDGCLW
                     YVGRSELMVKSSGFRFSPREIEGVVLGHSAVRDAAAWGVDDPALGQAVELAVVASGVQ
                     PAELLAYLRSALPRYMLPRRVHLL"
     gene            complement(1421..3367)
                     /gene="asnB"
                     /locus_tag="VF516_11585"
     CDS             complement(1421..3367)
                     /gene="asnB"
                     /locus_tag="VF516_11585"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8108364.1"
                     /translation="MCGIAGVVSRRTDGDALPPSPRAVDTEATVRAMLAVLHHRGPDD
                     RGVFVDAAAGLGHTRLAIIDPVGGVQPMRSRDDHLTLVFNGEVYNYVELRAELIQRGR
                     TFRTRSDTEVVLQAFAEWGDHAIERFNGQWAIALWDESRRRLVLCRDRVGIHPLYYTE
                     SADRVMFASEVKALFAGDPSLRAGLDPIGLDQTLTFWSAIAPRTVFRGISELPPGRIR
                     VYERGRVDERTYFEHDFTPTFAGSIADAERAVRTALERATALRMLRADVPVGCYLSGG
                     LDSSLIAALGLRAKGERFHTFSLRFADAEYDETEFQRLVARTLATEHHEVVVGRDDIA
                     RAFPAVVRHAERPLLRTAPAPMFLLSRTVAEAGIKVVLTGEGADEMFAGYDVFREGVV
                     RRFWAREPSSTLRPRLLQRLYPYLARSPVAQQAIAMQFFGRDLAAAGAPGFAHATRWT
                     TTAALKRLVAADLRVAGDAVADLCAELPAGFASWSSLAQDQYLEIRTLLGPYLLGSQG
                     DRMLMAHSVEGRFPFLDPEVVDLAHALPDAAKLRVLDEKHVVKRVARTLVPREIIARK
                     KQPYRAPDALSFVSPPAAPAIDELLSEAAVTAAGVLEPRSVAALWRKCRTSEGRVLSN
                     TDNMALVFAISTQLLCRELLGARL"
     gene            3401..3670
                     /locus_tag="VF516_11590"
     CDS             3401..3670
                     /locus_tag="VF516_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8108365.1"
                     /translation="MSNAGVPIEMVEQTISSFIVNNFLFGDAATAPARDLPLVQSGLV
                     DSTGILEIVTFLESEFGVHTSDEELAVGNFGSIETIARFVVAKQD"
     gene            complement(3679..4662)
                     /gene="nadE"
                     /locus_tag="VF516_11595"
     CDS             complement(3679..4662)
                     /gene="nadE"
                     /locus_tag="VF516_11595"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002048.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEX8108366.1"
                     /translation="MTFSPSVLDLDTHAEVERITAALRRQVGHVLRRRGAVIALSGGI
                     DSSVVAALCARAFGPARCHGLLMPERDSSDATLALSRSVAEGFGMPYTVEDITPALEA
                     LGCYRRRDEAIAEVVPDYGPGFRSKIVLPGLAAGAAYNLYSLVVERPDGTVERHRLPL
                     EPYLQIVAATNFKQRVRKTLEYYYADKLHYACTGTPNRLEYDQGFFVKNGDGAADVKP
                     IAHLYKSQVYALAEELGVPAEVRSRPPTTDTYSLAQGQDEFYFAVPYAQMDLCLYGKD
                     HGVAPAEVARATGLSPEQVTWVYDDIDKKRATTRYLHARPLLVDAVQNKAT"
     gene            complement(4673..6025)
                     /locus_tag="VF516_11600"
     CDS             complement(4673..6025)
                     /locus_tag="VF516_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX8108367.1"
                     /translation="MARTAVSRRRVVLGAMAAGAAGAVFAVRGRLRAMTQLASFAAPP
                     PLVPHDPVRDRTTIHVARGASPAANVDAVLDKVGGISTVVGADDLVIIKVSAQWWNQG
                     MTNVAAVKRVIEQVLERPGFRGEVIVFENTHFRLADGSGLARAWVHPSGRNVDVPGWT
                     RLGDLIPHFRARSAPVSFVGLVDAGPSSLSEDDWHDPGHACGVYGGDGRGPIAPGDPR
                     DGYHWDLGRTFRLRKTWFDDARTPLTWPRFTSPATGLVVDLQTGVLRREAGRLISTDR
                     KLTWINLTTANEHQATGFTGACKSTMGVVDMSAGALGTDPRVRGYSSVHYFGQGRPSA
                     SWRMAGPLAQFAREVRAPDLILTVAEWVAFAPVTPTDEDLRHSAATCTRARTIVAGRD
                     PVAIDTWIVRNLMAATPSANRRAHFDLDDPDAKFTKFARYYRQVRGAGTLDPGLITIT
                     "
     gene            6180..7298
                     /locus_tag="VF516_11605"
     CDS             6180..7298
                     /locus_tag="VF516_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108368.1"
                     /translation="MSAEHGIQYDVVASNPELVGPELYRLWCENLTLCTTPQAHFQRL
                     YRDAPEPTSTVFLLRAEASEPADVEIVGSNGFSVRRFQLGTKIEGRAAVSGDLVVAQG
                     HRSLAPALRLVRAVREHAMNEFALCYGFPNDKAKGVMVRAGFHVLGMMTRYARVLRHA
                     TYLPRVAARLPAPPRLATIIHSAAHHPLVRGLAPLLDVARLASELPGIAHARARHRLA
                     VLDEPDARFDELWARARSEYDVIGERTAAMLRWRYPRGTLAALVRSSDHALLAYALFE
                     LDDARTAHIRDVFGHKSALGPLFDLLLPTLWRRGAVSASIRMLGAPYLAAMVRARGFE
                     PRPDQRTIVVQVGREHETLRARLEDAAAWHMLDLDEDT"
     gene            complement(7336..8118)
                     /locus_tag="VF516_11610"
     CDS             complement(7336..8118)
                     /locus_tag="VF516_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108369.1"
                     /translation="MTTIDFVDVATARNAPGVRIVVSGLVPSPWAEATKGLFRIANVP
                     VLAVRRGRDAAEITAWTGVDNVPVVFHAAEPARTSWAAITTLAARLAGPDVVIPEELG
                     ARIEAMGLLHEIAGEHGIGWNARLAMIDASITSEGKRGFPLPVGQYLARRYGYTPDAA
                     ALGRARIERQLHALHDRLRAQQALGHTYLAGPRVSALDVYLATFLTPLSDITPEDCPQ
                     LEPVLRRAFGCAHEAFGSLVPAELRAHRRLVFERHLGWPIAL"
     gene            complement(8115..8288)
                     /locus_tag="VF516_11615"
     CDS             complement(8115..8288)
                     /locus_tag="VF516_11615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108370.1"
                     /translation="MKRGRGHWCWSCQQPKPNEAFSGRNHGPFRQVHAFFVVHVARGA
                     RASCAAARLLSAA"
     gene            complement(8285..>8763)
                     /locus_tag="VF516_11620"
     CDS             complement(8285..>8763)
                     /locus_tag="VF516_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108371.1"
                     /translation="VGVAVARRRDRERARIDEYFASLIAEAKRPRRQVARGAIEARIA
                     ALHAEHTAKLRDLTARYTLRVRLEPIALVAVGMRVAEVRLRLRRRKGEREIALHVPPS
                     ARAPDALACVACPGTTRAPLLCDDALHILCETCAPDAAGRPRCPACRPGRASRSAS"
BASE COUNT         1346 a         3115 c         3076 g         1226 t
ORIGIN      
        1 tcgagcaggt gcacgcgacg cggcagcatg tagcgcggca gcgcggatcg cagatacgcg
       61 agcagctcgg ccggttgcac gccgctggcc accaccgcga gctcgaccgc ctgaccgagc
      121 gccgggtcgt cgacgcccca cgcggccgca tcccggacgg cggagtgccc gaggacgacg
      181 ccctcgatct cgcgcgggct gaaccggaaa ccggaggact tgaccatcag ctcggagcgg
      241 ccgacgtacc acaggcagcc atcgtcatca cgccgcacca gatcgccggt gcgcagcacg
      301 cgccgctcac cgtcgcgata gaacttcgcc gccgtggcct gggcgtcctc ccagtagccc
      361 gccgcgacga gcgggccggc atggtgaagc tcgccggtga cgcccggcgg acacggtgtg
      421 ccgtccggtg cgacgacgcg gacctcgacc tccgggatgg gataccccat cgcgccgagc
      481 ttggcgtcga gctgcgcggc cggcagatag gtcgtgcgga agcattcggt ctgcccgtac
      541 atcaggacga tctgcgcgcc gggtagcgcg cggcgcatgc gctcgatcag cgccggcgac
      601 gggcgatcac cggcgttggt gacgtagcgc agcccggtga gctgccggcc ggtgtgctcg
      661 aggaagctga ccagctggtc ccagccgaac ggaacctgcg cgagggcggt cgcgccatgg
      721 tcggcgtgcg ccgtcacgac atcggcggcg agcaccgacg ccgggacgat cagccggccg
      781 ccggcctcgc acatcgtgag cagctgcgag agtccgtagc cgaagttcca gccgagcagc
      841 cacagcaggc ggtcgtctgc ggtgtttccg agatagcccg cgacgatccg cgcgaagtcc
      901 tcgaggttgc ggtgggagtg gaccacgccc ttgggccgcc cggtcgagcc cgacgtgtag
      961 agcaggatcg cgggttcgtc gcagccatcc accgcatcgt cccaggcctc gacgagcgtg
     1021 tcccacgccg tgccctggtg agccgggccg cgaccacgga cgaggacgtg cggaggcgtg
     1081 cgtccggcga gctgctggac gcgcagctcg cccgtgatca ggacgcgcgc accgctgttc
     1141 gcgatcacgt gctcgatctg ctgcggcgtc cactgggggg aggcagcgac ggcgatcgct
     1201 ccgacgcgcg ctgcgccgaa cagcgccgcc acggcatccg ggccggtctt gagatcgatg
     1261 accacgcgat cgccacgcgc ggtcccggcc gcgcgcagcc aggcggccag cttcgcgctc
     1321 tcgcgcgcca catcgccgga cgacagcgta ccgccggcgg tgaccagcag cgggcgatcg
     1381 gcgccgcgcg cgaggtggcg ctcgagcagg cggaacaggc tcacagtcgc gctccgagca
     1441 gctcccggca cagcagctgc gtcgagatcg caaacacgag cgccatgttg tcggtgttcg
     1501 acagcacacg cccctccgag gtccggcact tgcgccacag tgccgcgacc gatcgcggct
     1561 cgagcacgcc ggccgccgtc accgcggcct cggacaagag ctcgtcgatc gcgggcgccg
     1621 ccggcggcga gacgaacgac agcgcgtccg gcgcacgata gggctgcttc ttgcgagcga
     1681 tgatctcgcg cggcaccagc gttcgggcga cccgcttgac cacatgcttc tcgtcgagca
     1741 cgcgcagctt cgcagcgtcc ggcagggcgt gggcgagatc gacgacctcg ggatcgagga
     1801 atggaaaccg gccctcgacg gagtgcgcca tcagcatgcg gtcgccctgc gatccgagca
     1861 ggtagggacc cagcagcgtc cggatctcga ggtactggtc ctgtgccagc gagctccagg
     1921 acgcgaagcc ggcggggagc tcggcgcaca ggtccgccac ggcatcgccg gccacgcgca
     1981 ggtccgccgc gaccagccgc ttgagcgccg ccgtcgtggt ccaccgcgtg gcgtgcgcga
     2041 agccgggcgc accggcggct gcgagatcgc gcccgaagaa ctgcatcgcg atcgcctgct
     2101 gcgcgaccgg cgagcgcgcg aggtacggat acagccgctg gagcaggcgc ggccgcaggg
     2161 tcgacgacgg ctcgcgcgcc cagaacctcc gcaccacgcc ctcgcggaac acatcgtagc
     2221 cggcgaacat ctcgtcggcg ccctccccgg tgagcacgac cttgatgccg gcctcggcga
     2281 cggtccggga cagcaggaac atcggggcgg gcgcggtgcg cagcagcgga cgctcggcat
     2341 ggcggaccac cgcaggaaac gcgcgcgcga tgtcgtcgcg gccgaccacg acctcgtgat
     2401 gctcggtcgc gagcgtgcgc gcgaccagac gctggaactc ggtctcgtcg tactcggcgt
     2461 cggcgaaccg cagcgagaac gtgtgaaacc gctcgccctt cgcgcgcagg ccgagcgcgg
     2521 cgatcagcga gctgtcgagc ccgccggaca ggtagcagcc caccggcacg tcggcgcgca
     2581 gcatgcgcag cgccgttgcg cgctcgagcg ccgtgcgaac ggcacgctcg gcgtcggcga
     2641 tcgagcccgc gaacgtgggc gtgaagtcgt gctcgaagta ggtccgctcg tcgacgcggc
     2701 cgcgctcgta gacccggatc cggccgggcg gcagctccga gatgccgcgg aacaccgtgc
     2761 gcggtgcgat cgccgaccag aacgtcagcg tctgatcgag cccgatcgga tcgagcccgg
     2821 cgcgaagcga cggatcgccc gcgaacaacg ccttgacctc gctggcgaac atcacgcggt
     2881 cggccgactc ggtgtagtac agcggatgaa tccccacgcg gtcgcggcac agcaccaggc
     2941 gccgccggga ctcatcccac agcgcgatcg cccactggcc attgaaccgc tcgatcgcat
     3001 ggtcacccca ctcggcgaag gcctgcagca cgacctcggt gtcgctccgc gtccggaacg
     3061 tgcgaccccg ctggatcagc tcggcgcgga gttcgacgta gttgtagact tcgccattga
     3121 acacgagagt caggtggtcg tcacgactcc gcatcggctg cacgccaccg accggatcga
     3181 tgatcgcgag gcgtgtatgt ccgagaccgg cggctgcgtc gacgaacacg ccgcgatcgt
     3241 cgggcccccg gtgatgcagc accgcgagca tcgcccggac ggtggcctcg gtgtctacag
     3301 cacgcggact cggtggtaat gcatcaccat cagtacgacg actcaccact cctgcaatgc
     3361 cacacatggt cacggagctc ctctgagcta aaatggtttc atgtctaatg cgggcgtgcc
     3421 catcgagatg gtcgaacaaa caatctccag cttcatcgtg aacaacttcc tgttcggcga
     3481 tgctgccacg gctccggcgc gggacctgcc gctcgtgcag agcggactgg tcgactcgac
     3541 cggcatcctc gagatcgtca cattcctcga gtccgagttc ggcgtgcaca cctccgacga
     3601 ggagctcgcg gtcggcaact tcggctcgat cgagacgatc gcgcggttcg tggtcgccaa
     3661 gcaggactga atcggcattc aggtcgcctt gttctgcacc gcgtcgacca gcagcggccg
     3721 cgcgtgcaga tagcgggtgg tcgcgcgctt cttgtcgatg tcgtcgtaga cccacgtgac
     3781 ctgctcgggc gagaggccgg tggcgcgcgc gacctccgcc ggagcgacgc cgtgatcctt
     3841 gccatacagg cagaggtcca tctgcgcgta cggcacggcg aagtagaact cgtcctggcc
     3901 ctgcgccagc gagtaggtgt cggtggtcgg cggccggctg cgcacctccg cggggacgcc
     3961 gagctcctcg gcgagcgcgt agacctgcga cttgtagagg tgtgcgatcg gcttgacgtc
     4021 ggcggcgcca tcgccgttct tgacgaagaa gccctgatcg tactcgagcc ggttcggcgt
     4081 cccggtgcac gcgtagtgca gcttgtccgc gtagtagtac tcgagcgtct tgcgcacgcg
     4141 ctgcttgaag ttcgtggcgg cgacgatctg gaggtacggc tcgagcggga gccggtggcg
     4201 ctcgaccgtg ccgtcgggcc gctcgacgac gagcgagtac aggttgtagg ccgcgcctgc
     4261 cgccagcccc ggcagcacga tcttcgatcg aaagccgggg ccgtagtcgg gaacgacctc
     4321 ggcgatcgcc tcgtcgcgcc gccggtagca gccgagcgcc tcgagcgccg gcgtgatgtc
     4381 ctcgacggta tacggcatgc cgaatccttc ggccaccgag cgcgacagcg cgagcgtcgc
     4441 gtccgacgag tcgcgctccg gcatcagcag gccgtggcag cgcgccgggc cgaaggctcg
     4501 cgcacacagc gcggcgacca cgctcgagtc gatgccgccc gacagcgcga tcacggcgcc
     4561 gcggcggcgc aggacgtgtc cgacttgccg gcgcaacgcc gccgtgatcc gttcgacttc
     4621 cgcatgagtg tcgagatcga gaacgctggg ggagaatgtc atggtggtga tctcacgtga
     4681 tggtgatcag gccgggatcg agcgtgcccg cgccgcggac ctgccggtag tagcgggcga
     4741 acttggtgaa cttcgcatcg gggtcgtcga gatcgaagtg agccctgcgg ttggcgctcg
     4801 gggtcgccgc catcaagttg cggacgatcc acgtgtcgat cgcgacggga tcgcggcctg
     4861 ccacgatcgt ccgggcccgg gtgcacgtgg ccgccgagtg gcgcaggtct tcgtcggtcg
     4921 gggtgaccgg cgcgaacgcg acccactcgg cgacggtgag gatcaggtcg ggagcgcgca
     4981 cctcgcgcgc gaactgcgcg agcgggccgg ccatccgcca gctcgcgctc ggcctgccct
     5041 ggccgaagta gtgcacggac gaatagccgc gcacccgcgg atcggtgccc agcgcacctg
     5101 cgctcatgtc gaccacgccc atcgtcgact tgcaggcgcc ggtgaacccg gtcgcctggt
     5161 gctcgttcgc ggtcgtcagg ttgatccacg tcagcttgcg gtcggtcgag atcagccggc
     5221 ccgcctcgcg acgcagcacg ccggtctgga gatcgacgac caggccggtc gcgggagacg
     5281 tgaagcgcgg ccacgtcagc ggggtcctgg catcgtcgaa ccaggtcttg cggaggcgaa
     5341 acgtgcggcc gagatcccag tggtagccat cgcgcggatc gcccggcgcg atcggtccgc
     5401 gaccgtcgcc gccatagacc ccgcaggcgt gcccgggatc gtgccagtcg tcctccgaca
     5461 gagagctcgg acccgcatcg accagaccga cgaagctcac cggcgcgctg cgcgcccgga
     5521 agtgcgggat caggtcgcca agccgggtcc agcccggcac gtcgacgttg cgcccggacg
     5581 ggtgcaccca cgcgcgcgcc aggcccgagc cgtcggcgag ccggaagtgg gtgttctcga
     5641 aaacgatcac ctcgccgcga aacccggggc gctcgaggac ctgctcgatc acgcgcttga
     5701 ccgccgcgac gttggtcatt ccctgattcc accactgcgc gctgaccttg atgatcacca
     5761 gatcatcggc accgaccact gtcgagatac cgccgacctt gtcgagtacg gcgtcgacgt
     5821 ttgccgccgg ggaagcgcct cgtgcaacgt ggatcgtggt gcgatcgcgc acgggatcgt
     5881 gcggcaccag cggaggtggt gcggcgaacg acgcgagctg ggtcatcgcg cgcagcctgc
     5941 cgcgcaccgc gaacaccgcg ccggcggccc ccgcggccat cgcgccgagc accacgcggc
     6001 gtctcgatac cgcggttctc gccatcaccg tcgtcagtgt agccgggcac ctgcccaagt
     6061 gaagcaccaa ctcgaggcgc gacccgatag accgcactcc tgcaatgttc tgccgtgctc
     6121 gaccacggac tgccgcaaca tgacgccgcg cccgacgatg acccgggcca cgctccttta
     6181 tgtcagcaga gcacggtatc cagtacgacg tggtcgcctc gaatccggaa cttgtcggtc
     6241 ccgagctata tcggctgtgg tgcgagaacc tcacgctgtg caccactccc caggcgcatt
     6301 tccagcggct gtatcgcgat gcgcccgagc caacctcgac ggtgttcctg ctgcgcgccg
     6361 aggcgtccga gcccgcggat gtggaaatcg tcggcagcaa cggcttctcg gtccgccggt
     6421 tccagctcgg cacaaaaata gaaggtcgcg cggcggtcag cggcgatctc gtggtggcgc
     6481 aagggcatcg cagcctggcc ccggcgctcc gcctggtccg ggccgtccgc gagcacgcga
     6541 tgaacgagtt cgcgctttgc tatggatttc cgaacgacaa ggccaagggc gtgatggtgc
     6601 gtgccgggtt tcatgtgctc ggtatgatga cgcggtacgc acgagtgctg cggcatgcga
     6661 cctatctccc gcgggtcgcc gcgcgcctcc cggctccgcc tcggctcgcg acgatcatcc
     6721 actcggctgc tcaccatccg ctcgttcgcg ggctcgcgcc gctgctcgat gtcgcacggc
     6781 tcgcgagcga gctccccggg atcgcccatg cgcgcgcccg ccatcgactc gcagtgctcg
     6841 acgaacccga cgcacggttc gacgagctct gggcgcgcgc gcgctcggag tacgacgtga
     6901 tcggcgagcg gaccgcggcg atgctgcgct ggcgctaccc gcgcggcacg ctcgcggccc
     6961 tcgtgcggag ctccgatcac gcgctcctgg cctacgcgct gttcgagctc gacgacgccc
     7021 ggaccgcgca catccgcgac gtgttcggcc acaagtcagc gctcgggccc ctgttcgatc
     7081 tgctgctccc cacgctgtgg cggcgcggcg ccgtcagcgc ctcgatccgc atgctcggtg
     7141 cgccctacct cgcggcgatg gtccgcgcgc gtggcttcga gcctcgcccc gatcagcgca
     7201 ccatcgtcgt gcaggtcggg cgcgagcacg agacgctgcg ggctcgactc gaggacgccg
     7261 ccgcctggca catgctggac ctcgacgagg acacctgaac cgcctgcgat cgaatcacgt
     7321 gctggctcgg cgcggtcaca gcgcgatcgg ccagccgagg tggcgctcga acaccagcct
     7381 gcgatgcgcc cggagctcgg ccgggacgag cgacccgaac gcctcgtggg cgcagccgaa
     7441 cgcccgacgc agcaccggct cgagctgcgg gcaatcctcg ggcgtgatgt ccgatagcgg
     7501 cgtgaggaac gtcgccaggt acacgtcgag cgccgacacc cgcggaccgg cgaggtacgt
     7561 gtggccgagc gcctgctgcg cgcgcagccg gtcgtgcagc gcatggagct ggcgctcgat
     7621 ccgggcgcgg ccgagcgccg ccgcgtcggg ggtgtagccg tagcgccggg cgaggtactg
     7681 gccgaccggc agcgggaagc cgcgcttgcc ctcgctggtg atcgaggcgt cgatcatcgc
     7741 gagccgcgcg ttccagccga tgccgtgctc gccggcgatc tcgtggagca ggcccatcgc
     7801 ctcgatccgc gcgccgagct cctcggggat caccacgtcg gggccggcca gccgcgcggc
     7861 gagcgtggtg atcgccgccc agctggtgcg cgccggctcg gccgcgtgga acacgaccgg
     7921 gacgttgtcg accccggtcc acgcggtgat ctcggcggcg tcgcggccgc ggcgaaccgc
     7981 cagcaccggc acgttcgcga tccggaacag ccccttggtc gcctcggccc acgggctcgg
     8041 caccaggccg gacacgacga tccgcacgcc gggcgcgttg cgcgccgtcg caacgtcgac
     8101 gaagtcgatg gtagtcatgc ggccgacagt agccgagctg ccgcgcaaga agcgcgcgcg
     8161 ccgcgcgcca catggacgac gaagaacgcg tggacctggc ggaacgggcc atggttgcgg
     8221 cccgagaacg cctcgttcgg cttgggctgc tggcagctcc agcaccagtg gccgcgaccg
     8281 cgcttcatga ggcgctcctc gacgcgcggc ccggccgaca tgcgggacac cgagggcgcc
     8341 ccgcggcgtc cggcgcgcag gtctcgcaga ggatgtgcaa tgcgtcgtcg cacagcagcg
     8401 gggcccgcgt cgtgcccggg catgcaacgc acgccagggc gtctggagcc cgcgcggacg
     8461 gcggcacatg cagcgcgatc tctcgctcgc ccttgcgccg gcgcagccgg aggcgcacct
     8521 cggcgacccg catcccgacc gccaccagcg cgatgggctc gagccgcacg cgcagggtgt
     8581 agcgagcggt cagatcgcgc agcttcgcgg tgtgctcggc gtgcagcgcc gcgatgcgcg
     8641 cctcgatcgc acctcgtgcg acctggcgcc tcggacgctt ggcctccgcg atcagcgacg
     8701 cgaagtactc gtcgatgcgc gcgcgctcgc ggtcgcggcg gcgagcgacg gccactccga
     8761 cct
//
LOCUS       DASVOR010000589         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_210089,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000589 DASVOR010000000
VERSION     DASVOR010000589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210089"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..233)
                     /locus_tag="VF516_11625"
     CDS             complement(<1..233)
                     /locus_tag="VF516_11625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108372.1"
                     /translation="MARRHPPPRPPRVVRLGAVAVAMAVVVALGGAIAPPVGHAEPAP
                     DLERARELYRSAEAAMQDGRYDDAARDYGAAYEL"
     gene            430..1236
                     /locus_tag="VF516_11630"
     CDS             430..1236
                     /locus_tag="VF516_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108373.1"
                     /translation="MNGLDQRTDTGGTTSVAGPASTAAVGKQSLVDTARTGAPTAAGP
                     SAESTQAGATAAPDVDVGAPNVEGGGTTGGPLTKDKAKQILQDAFGKYKTITEGTVEV
                     LEQAAFQAAYDKIYGKTQYSWDKWVKPIHGNLNGFAYNGVNYINKAMANTGTVPHEML
                     HNNAASDWRPFVGSQFDEGATDVLKQFALKKAGLSSPNSYPDQISCVRAFLDSGVSED
                     QLFTAYLKSGAATIVGDHVDKTCAGSWADVKAAMEAKDWAKAKVKLAKKK"
     gene            1247..1783
                     /locus_tag="VF516_11635"
     CDS             1247..1783
                     /locus_tag="VF516_11635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108374.1"
                     /translation="MTVMINRHALVDQVAAGITPGLAIPDAARVRSLLDELVSDWYRE
                     AGNDSDAPDVIALLRKELDAALADKPAAVRMEIVRAAVLDRRVRRLGVQTLALGKQLI
                     DGTIAQEDARAQGESLMKQIEEVAAEVRTLADAGARARLARDVQEASMEALYAIEGKA
                     MSHRLSHYARDTQPPRVF"
     gene            1941..2435
                     /locus_tag="VF516_11640"
     CDS             1941..2435
                     /locus_tag="VF516_11640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108375.1"
                     /translation="MAGPVAQTQQVELHQLAGDLAADWRREAGNDKDAADVLALLRSE
                     LATKLTAARGHASDANLEVLRAEVYDRRARALSRRSLALGKAVIDGAIDDAAGRRDGE
                     QILREVAVLARQVHALHDPERVRVLSAALQEVSLEASFAVARGPTSRRLDDYRTSRQG
                     AAGR"
BASE COUNT          437 a          855 c          945 g          348 t
ORIGIN      
        1 agctcgtagg cggcgccgta gtcgcgggcg gcgtcgtcgt agcggccgtc ctgcatcgcg
       61 gcctcggccg accggtacag ctcccgggcg cgctcgaggt cgggggccgg ctcggcgtgg
      121 ccgaccgggg gggcgatcgc gccgccgagg gcaacaacga ccgccatggc gaccgccacc
      181 gcgcccagcc gcaccacgcg cggcgggcga ggcggaggat gacgccgggc catgcagcga
      241 gcgtaccgca accaagcgtt gccaccggcc ggacgtcgtg gcctgacgct gatttcgccg
      301 gttccaccgc ggccgtccgt gaggtgtgcc acccggagcg tcaaggtgcc gaaaggcttg
      361 ctagattctc ggcggtcagg gggtacgatc ggcgaggcag ttgttcgcgc ctcagccgta
      421 ctacgagcca tgaacgggct ggatcaacga acggatacgg gtggcacgac cagtgtggct
      481 gggccggcga gcacggcggc ggtcggcaag cagtcgttgg tcgacacggc gcggaccgga
      541 gctccgaccg ccgctggccc gagcgccgag tcgacccagg caggcgcgac cgcggcaccc
      601 gatgtcgacg tgggcgcgcc caacgtcgag gggggcggca cgacgggcgg tccgctcacc
      661 aaggacaagg ccaagcagat cctgcaggac gccttcggca agtacaagac gatcaccgaa
      721 ggcaccgtcg aggtgctcga acaagctgcg ttccaggcgg cgtacgacaa gatctatggc
      781 aagacccagt actcgtggga caagtgggtc aagccgattc acggcaatct gaacggcttt
      841 gcctacaacg gtgtcaacta catcaacaag gcaatggcga atactggaac ggtgccgcac
      901 gagatgctgc acaacaacgc cgcctccgac tggcggccgt tcgtgggctc gcagttcgat
      961 gagggtgcga ccgatgtgtt gaagcaattc gcgctgaaga aggccgggct cagctcgcca
     1021 aactcctatc ccgaccagat aagctgtgtc cgggcattcc tcgactccgg tgtctccgag
     1081 gatcagctgt tcacggcgta cctcaagagc ggcgcggcga ccatcgtcgg cgatcacgtc
     1141 gacaagacct gcgccggtag ctgggccgat gtgaaggccg cgatggaggc gaaggactgg
     1201 gcgaaggcga aggtcaaact ggccaagaag aagtgaggga atcacgatga ccgtcatgat
     1261 caatcgccac gcgctcgtcg atcaggtggc cgccggcatc acccccgggt tggcgatacc
     1321 cgacgccgct cgcgtgcgca gcctgctcga cgagctggtc agcgactggt accgcgaggc
     1381 cggcaatgac agcgatgcgc ccgacgtcat tgcgctgctg cgcaaggagc tggatgccgc
     1441 actcgccgac aagcctgccg cggtccgcat ggaaattgtg cgcgctgcgg tgctcgatcg
     1501 ccgggttcgt cggctcggcg tgcagaccct cgcactcggc aagcagctga tcgatggcac
     1561 cattgcgcag gaggatgcgc gggcccaggg cgagtcgctg atgaagcaga tcgaggaggt
     1621 cgccgccgag gtgcgcacgc tggccgatgc cggcgcccgt gcgcggctcg cccgcgatgt
     1681 ccaggaagcc agcatggagg cgctctatgc gatcgagggc aaggcgatga gtcatcgcct
     1741 cagccactac gcgcgtgaca ctcagccacc ccgcgtcttc tgagatgagg cgcatcggtg
     1801 ttgccatgtt gcttgccggg gcctgccgca gcgcagcgcc cgcaacgggt ggcggggcgc
     1861 cggctgtacc ggagccatcg cccgtggcag cttcggcagc gcctgcagca tcggacccgc
     1921 tcgctggcga gatcgccggg ttggcgggcc cggtcgcgca gacccagcag gtcgagctgc
     1981 accagctggc gggcgacctg gccgccgact ggcggcgcga ggctggtaac gataaggacg
     2041 cagccgatgt cctggcgctg cttcgcagcg agcttgcgac caagctcacc gccgcgcgcg
     2101 gccacgcttc cgacgcaaat ctcgaagtct tacgcgccga agtctacgac cgccgggccc
     2161 gggcgctgag ccggcggagc cttgcgctcg gcaaggcggt gatcgacggc gcgatcgacg
     2221 acgccgcggg tcgccgcgat ggcgaacaga tcctgcggga ggtggctgtg ctggcccggc
     2281 aggtccacgc gctgcacgac cccgagcgcg tgcgcgtgct gtcggccgca ctgcaggagg
     2341 tctcgctcga agcgagcttc gcggtcgcgc gcggcccgac gagccgtcgg ctcgacgact
     2401 accggacgtc gcggcagggt gccgcgggcc ggtgatcgcg ggatccgtga ggccgacgcg
     2461 ccgccgcgat gccgcctggg caacggtgtc cggacccgag cggcgctgac cagaaccgca
     2521 ccgagacccc gctgccgcgc aggccgacgc cgggggacgg cggggcgcgg ggcgggcgag
     2581 cgggt
//
LOCUS       DASVOR010000590         7661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_239104,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000590 DASVOR010000000
VERSION     DASVOR010000590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239104"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            368..889
                     /locus_tag="VF516_11645"
     CDS             368..889
                     /locus_tag="VF516_11645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108376.1"
                     /translation="MLAVIVIAGCTGQVTPEGGSPGDPSPDAGSSGSGGGGGGGNLDA
                     GMGDAAGFACRNQVASVGSGHHNPGQDCQGSCHNHGFTLGGTIFAAANSTAPLSGATI
                     TVTDATGKSFDIVSQANGNFYTSQAIKFPVTVTASECPSVAPMVAQVTATMDGCNQSG
                     CHVAGAQGRIHLP"
     gene            1281..2930
                     /locus_tag="VF516_11650"
     CDS             1281..2930
                     /locus_tag="VF516_11650"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8108377.1"
                     /translation="MGKLEKIRLPVLQAGSPPPPEVPLALAESMFLPPDVPAFDHAAS
                     RTGEERQLRTSSYVIWTPLPANDEDVLLIHGYTGAYDRVAKRVADYLRSLEVGKRAKP
                     LHGEWSPEPRPSAVPPPSADTLERLRKRGYLTRLSVEEEESLFTKFAAQRHFSAVRST
                     PSYIVMPTYECNLRCPYCFQDHMRTDASYRHLLRTMDVAMADRILQGMETIEIAHGLA
                     PEGPRTRNVTFFGGEPLLEQSLGIVRHFVDRLLERGKANISAVSNGTELEAYRDLLGP
                     DKIAFLQITIDGPPAVHDQRRIYADGSGSFERIARNIDLALDRGVKISVRMNIDRSNF
                     QLLPAIADEFRARGWTGRAGFSSYVAPVHMAGDSESKHKSFNSWQLSRALIELQQQHA
                     STRQIGGPDDGTVARVRQIFDRRSSPLPSFKADFCGAHTTMYIFDAFGDIYACWERTG
                     DPSVRIGSVTETGEVLMSSARMEAWRSRSVTSNPVCRKCRYATYCGGGCAILAEGEHG
                     DIHANHCDGFAQRFRRSAATAFTEHLAGAVNERNADRVCDL"
     gene            2985..3254
                     /locus_tag="VF516_11655"
     CDS             2985..3254
                     /locus_tag="VF516_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108378.1"
                     /translation="MSKKESDDSPRRELESLNADELDIEELERRIEMASTLPDGFAWL
                     CGVDCGNNCVGNCTSNCIGDCPSNCIGNCPANCVGNLCGSNSTEV"
     gene            3346..5175
                     /locus_tag="VF516_11660"
     CDS             3346..5175
                     /locus_tag="VF516_11660"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HEX8108379.1"
                     /translation="MFPANIQTEIDDAAGWEANLQRGWPDAVQLRVWRQSIWYELPYL
                     FLPAFPQVALADVRPLALFGRLLASSMFVHDPLADREVAVHDAAIATLRIMAMQFEAY
                     HALHGCVPARARFWDRLRSYLADYAEACTSELRVSAGAIPWCEFDDALALRIMTGKSG
                     ASRAIVAGLAELAHDDGPLEPLITSLDHFNIACQLWDDLKDWRDDLQHAMPSMLLARV
                     LPEHPRTCEDAREPDRVARMLYYDGHARAVLELALASLDAAERASEPFGVQAFHRLIA
                     LTRRRIEPTLHDLNRLVERNVERAVARPAHAQLDLMSLWQSLAWWGAQLLVRACQGKV
                     DLSDPLLRIHVVDALSDVADGLGGELDALLRAQRTHIAAADLVEVDVIAGAVRVALRA
                     DRGASTLERCAPLVDRLAREPALTSGAIDPAARGDASTDVVAEVVATLQAYDTTRFAE
                     RVQRGCAYLEHRQRPDGAWDGRPIRGLYHVTYACVRTLATCCPGSRALERALAYLRTC
                     QHADGGWGHPAASSDPQSTALALLGLAAGAPRWSQPDDHDRAARALQFLGSARAVDGV
                     WAAPSKAGDATFEGSETVTTALVVKAAIAWHSRWTASRGERSA"
     gene            5130..6365
                     /locus_tag="VF516_11665"
     CDS             5130..6365
                     /locus_tag="VF516_11665"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HEX8108380.1"
                     /translation="MAQQVDGEPRRAIRMKALFCPLATPGFVYPAIAVARSLQRRGHQ
                     AAFVTGPAYQETLAQAELVRIPRGRTDGSSFAVERWFHPLDIAMQAKHIEYALDRFAP
                     DVIVGHPLSLGTLIVRARARVPTAVLGMAVQLWPVPGASAETDHETARRLAWRQVVTM
                     GYLNDARKLFQLGPCDDDPLLGDLFMLQSVPELGIDVDHSPPHVHLVGACLWEPPATQ
                     DQLAWLDAARAAGEPLIYVHHGRSFDRPSFLPLVVDALGGRRVRVAAAVGRMDHAVGD
                     PPPNFLVRPHLCQGRILPHARLVISGGNTTAVLGALCHGVPALLLPGDGAEQLDTAEL
                     VARAGSGRLLPVDQATAPGLARAVDELLAPGDHAHRAQDLARAFARWDGPDRAAQLVE
                     RLAGSRSHVPREPEGAGCH"
     gene            6356..7558
                     /locus_tag="VF516_11670"
     CDS             6356..7558
                     /locus_tag="VF516_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108381.1"
                     /translation="MPLTELRGLDERLVVDTSEARPVFLLAGSNGGYLRISEATRHFL
                     QRVQQGTSFEELARSMRRQDGSPLSAEEVESKYRQLTDQIARFEEGGSEPKGAFWFRR
                     RLVRGATIARLAALCSAALRPAVAVVLVAAIVAAVVAATRLVTPTAPKQFWLSYAVFL
                     VSLLVHELGHASACVRFGGKPSDIGIVIYLLYPSFYSDVTVAWEFRRHQRVVVDLAGV
                     YFQLIFGAGCVAVYAVTGWEPLEGALLFIMGSCLFSLNPILKFDGYWVVADALGVTNL
                     GRQRMRLTRHLLALARGRAVRALPWTRFVSIALALYTVVALGFWVWFLWRLCPTIGHQ
                     IVRWGEFAAAFGRELIFAQSGLAWETTKNLLTSTFVLLFVVFMTGRLLAPAVRWVRLR
                     LGTGRSPA"
BASE COUNT         1139 a         2691 c         2636 g         1195 t
ORIGIN      
        1 gccggccggc tcggcgtgtc cggagcccag cccgccgacg tcctgctcgg ggcgctcgac
       61 cgcgcgtggc gcgagctccg cgcgccggag atcgtcccga tcgtcgaggg cgcggtgtgc
      121 ggccccgacg gctgcgactg atcgtcgacc gaacctcgac cgatcctcga cgaggctgag
      181 ggggagaccc ccaccctcgg tgatcgaccg cacagcccgc acgagcttcg tacacgctgt
      241 acgccggctg cacgcgagcc gctcgatctc gtcggcgtga cagccagtta tggcggccgc
      301 gcggcggtct gcgcgttgca ccgcaccggc tcgccgcgat cgcggcggag gacgcatgcg
      361 acacctcgtg ctggcagtca tcgtgatcgc gggatgtacc ggccaggtga ctcccgaagg
      421 gggaagccct ggcgacccgt ctccggacgc cggcagcagc ggcagcggtg gtgggggagg
      481 cggcggcaac ctcgacgccg ggatgggcga cgccgccggc ttcgcgtgcc gcaaccaggt
      541 cgccagcgtg ggctccggcc atcacaaccc cgggcaggac tgccaggggt cgtgccacaa
      601 ccacggcttc acgctcggcg ggacgatctt cgccgccgcg aacagcaccg cgccgctgtc
      661 cggcgccacg atcaccgtca ccgacgccac cggcaagtcg ttcgacatcg tcagccaggc
      721 caacggcaac ttctacacct cgcaggcgat caagttcccg gtcacggtga cggcgagcga
      781 gtgccccagc gttgccccga tggttgcgca ggtcaccgcg accatggacg gctgcaacca
      841 gagcggctgt cacgtcgccg gcgcgcaggg ccggatccac ctgccgtagc ccggagaatc
      901 accacaccgc cggctgcgac cgtcgcggcg cgggcatcct gtcccgtgcc gaaagtccgc
      961 cggcgggaaa cgcgaggcgc ctccgcggct tggcgcgccg ccggaccggg cgtcctgccg
     1021 tcgccatcga cgtagagcag atcgtagtcc ggatggcgac ggagaatcct tcctgggatc
     1081 aaggcgatcg cgctcgcgac ctggcaggcc gacacgccgg cgatcgacgc ggtgccggtg
     1141 ccgcacgggg tagtctcggt cgacctgatc actggatgat ggctccgtga gtggatgacg
     1201 gctccgtgag ttagccctat tgtatagaca ggcgtgtagc catagtgata tatctggtcg
     1261 gacattgaag gcgctcttac atgggtaagc ttgagaaaat ccggctcccg gtcctgcaag
     1321 caggttctcc gccccccccg gaggtcccgc tcgcgctggc agagtcgatg ttcctgccac
     1381 ccgacgttcc tgcattcgat cacgctgcca gccgcacggg cgaggagcgg caactgcgga
     1441 cgagcagcta cgtcatctgg actccgctgc ccgccaacga cgaagacgtc ctcctgatcc
     1501 acggctacac gggcgcgtac gaccgggtcg ccaagcgggt cgccgactac ctccgcagcc
     1561 tcgaggttgg caaacgcgcc aagccgctcc acggcgagtg gagccccgag ccccgcccga
     1621 gcgctgtccc gccaccgtct gccgacacac tcgagcggct cagaaagcgc ggctatctga
     1681 cccggctgtc ggtcgaggag gaggagtctc tgtttaccaa gttcgccgcg cagcgtcact
     1741 tctccgctgt gcgcagcacg ccgagctaca ttgtgatgcc gacgtacgag tgcaatttgc
     1801 gttgcccgta ctgctttcag gatcacatgc ggacggatgc ctcgtatcgg catctgctcc
     1861 gcacgatgga cgtcgcgatg gcggatcgca tcctccaagg gatggagacg atcgagatcg
     1921 ctcacggcct cgcccctgag ggcccgcgca cgcgcaatgt cacgttcttc ggtggtgagc
     1981 cgttgctcga acagagcctc gggatcgtcc gacacttcgt cgatcggctg ctcgagcgcg
     2041 gcaaggcgaa catctccgcg gtgagcaacg gcaccgagct cgaggcgtat cgggacctcc
     2101 tcggtcccga caagatcgcg ttcctccaga tcacgatcga tggcccaccc gcagtccacg
     2161 atcagcgacg gatctacgcc gatggctcag gctcgttcga gcggatcgcg cggaacatcg
     2221 accttgcgct ggatcgcggc gtgaagatca gcgtccggat gaacatcgat cgcagcaact
     2281 tccagctact gcccgcgatc gccgatgagt tccgggcgcg cggctggacc gggcgcgcgg
     2341 gcttctcgtc ctacgtcgcc ccggtccaca tggcgggcga ctcggagagc aagcacaagt
     2401 cgttcaactc ttggcagctg tctcgcgccc tcatcgagtt gcagcagcag cacgcctcca
     2461 cccgccagat cggcgggccg gacgacggga cagtcgcgcg cgtccgccag atcttcgaca
     2521 gacgctccag cccactcccc agcttcaagg ccgacttctg cggcgctcac accacgatgt
     2581 acatcttcga tgcgttcggc gacatctacg cctgctggga gcgcaccggc gacccgagcg
     2641 tccgcatcgg ctcggtcacg gagacgggcg aggtgctgat gagcagcgcc cggatggaag
     2701 cctggcgcag ccggagcgtg acctcgaacc cggtgtgccg caagtgtcgc tatgcgacgt
     2761 actgcggcgg aggctgcgcc atcctcgccg agggcgagca tggcgacatc cacgccaacc
     2821 actgcgacgg attcgcccag cggttccgcc gcagcgccgc caccgcgttc accgagcacc
     2881 tcgcgggcgc ggtgaacgag cgaaacgccg atcgggtctg tgatctctga tcgctcgtcg
     2941 ctcaccgcca gcgaccgctg ccaccaccac cggagtacaa cacaatgagc aagaaagagt
     3001 cagacgacag cccccggaga gaactcgaga gcctcaacgc ggacgagctc gatattgagg
     3061 agctcgagcg ccgcatcgag atggcctcga cgctccccga tggctttgcg tggctctgcg
     3121 gagtggactg cggaaacaac tgtgtcggga actgtacgag caactgcatc ggcgactgcc
     3181 ccagcaactg catcggcaac tgccccgcga actgtgtggg aaatttgtgc gggagcaaca
     3241 gcaccgaggt ctagtgctcg gcgcctctcc gcgataggct cggggccgca tccgagctcg
     3301 actgcggcgc ggcgcacggt atgagcggcc accgcggagc accccgtgtt cccagccaac
     3361 atccagaccg agatcgacga cgcggccggc tgggaagcca acctgcagcg cgggtggcca
     3421 gacgcggtgc agctccgcgt ctggcggcag tcgatctggt atgagcttcc gtatcttttc
     3481 cttcccgcgt tcccgcaggt ggcgctcgcc gacgtccggc cgctcgcctt gttcggtcgg
     3541 ctgctcgcga gctccatgtt cgtccacgat ccgctcgcgg accgcgaggt cgcggtgcac
     3601 gacgccgcga tcgcaacgct gcggatcatg gcgatgcagt tcgaagccta ccacgcgctc
     3661 cacggatgtg tcccggcgcg cgcgcggttc tgggatcgcc tgcggtcgta tctggccgac
     3721 tacgcggagg cgtgcacgag tgagctgcgg gtttcggccg gcgcgatccc gtggtgcgaa
     3781 ttcgacgacg cgctggccct gcggatcatg accggcaaga gcggcgcctc acgggcgatc
     3841 gtcgccgggc tggccgagct cgcccacgat gacgggccgc tcgagccgct gatcacgtcg
     3901 ctcgaccact tcaacatcgc gtgtcagctg tgggacgacc tcaaggactg gagggacgac
     3961 ctccagcacg cgatgccgtc catgctgctc gcacgcgtcc tgcctgaaca cccgaggacc
     4021 tgcgaggacg cgcgggaacc cgatcgagtc gcgcgcatgc tctattatga cggacatgcc
     4081 cgtgcggtgc tcgagctggc cctggcttcg ctcgacgcgg cggagcgcgc tagcgagccg
     4141 ttcggcgtcc aagcatttca ccggctgatc gcgctgacgc ggcggcggat cgagccgacc
     4201 ctgcacgacc tcaaccggct cgtcgagcgc aacgtcgagc gcgcggtcgc gcggccggcg
     4261 cacgcacagc tcgatctgat gagcctgtgg cagtccctgg cgtggtgggg agcccagctc
     4321 ctcgttcgag cgtgccaggg caaggtcgac ctgagcgacc cgctgctgcg catccacgtg
     4381 gtagacgcgc tgagcgacgt ggcggatggg cttggtggcg aactcgacgc gctcctccgc
     4441 gcgcagcgca cccacatcgc ggcggccgac ctggtggagg tggatgtgat cgctggggcg
     4501 gtgcgggtcg cgctgcgagc ggaccgcggt gcgtcgacgc tcgagcgctg cgctcctctc
     4561 gtggaccgac tggccaggga acccgcgctg acctccggcg cgatcgaccc cgcggcccga
     4621 ggcgacgcca gcaccgacgt ggtggcggag gtggtggcca cgctgcaggc gtatgacacc
     4681 acgcggttcg ccgagcgcgt ccagcgcggc tgcgcgtacc tcgaacaccg gcagcgcccc
     4741 gatggcgctt gggacggccg accgatccgg ggcctgtacc acgtgaccta cgcctgcgta
     4801 cggaccctgg cgacgtgctg tccaggctcg cgcgcccttg agcgcgcgct ggcgtacctg
     4861 aggacctgcc aacacgcgga tggcggatgg ggccacccgg ccgcctccag cgatccccag
     4921 agcaccgcgc tggcactgct cgggctggcc gcgggggcgc cacgctggag ccagccggac
     4981 gatcacgacc gggcggcacg cgcgctccag tttctcggct cggctcgcgc ggtcgacggg
     5041 gtatgggccg cgccgagcaa ggccggcgac gccacgttcg agggcagcga gaccgtgacg
     5101 actgccctgg tggtgaaggc cgcgatcgcg tggcacagca ggtggacggc gagccgcggc
     5161 gagcgatccg catgaaggcg ctgttctgcc cgctcgccac gccgggcttt gtctatccgg
     5221 cgatcgccgt cgcgcgctcg ctccagcggc gcggccacca ggccgcgttc gtcaccggcc
     5281 cggcctacca ggagaccctg gcgcaggcgg agctcgtgcg aattccgcgc ggccggaccg
     5341 acgggtcgag cttcgcggtc gagcgatggt tccacccgct ggacatcgcc atgcaggcca
     5401 agcacatcga gtatgcgctc gaccgcttcg cgcccgacgt gatcgtaggc catccgctgt
     5461 cgctcggcac gctgatcgtg cgtgcgcgcg cccgcgtacc gactgccgtc ctgggcatgg
     5521 cggtccagct gtggccggtc ccgggcgcct cggccgagac cgatcacgag accgcgcgac
     5581 ggttggcgtg gcgccaggtg gtgacgatgg gttacctgaa cgacgcgcgc aagctgttcc
     5641 agctgggacc gtgcgacgac gacccgctgc tcggcgacct gttcatgctg cagagcgtgc
     5701 ccgagctcgg gatcgacgtg gatcactccc caccgcacgt ccacctggta ggcgcctgtc
     5761 tgtgggagcc cccggcgacc caggaccagc tcgcgtggct cgacgcggcc cgcgcggcgg
     5821 gggagcccct gatctacgtc caccatggcc gatcgttcga tcggccgagc ttcctgccgc
     5881 tggtcgtcga cgcgctcggc ggccggcggg tccgggtcgc ggccgccgtc ggacgcatgg
     5941 accacgcggt cggcgatcca ccgcccaact tcctcgtccg tccgcacctc tgccagggac
     6001 ggatcctgcc acacgcgcga cttgtgatca gcggcggcaa cacgaccgcg gtgctcgggg
     6061 cgctctgcca cggggtgcct gcgctgctgc tcccgggcga cggcgccgag cagctcgaca
     6121 ccgccgagct cgtcgcgcgc gcgggtagcg ggcggctact cccggtcgac caggcgaccg
     6181 cacccggcct ggcgcgtgcg gtggacgagc tgcttgcgcc cggcgaccac gcgcaccgcg
     6241 cgcaggacct cgcacgcgcg tttgcccgct gggacgggcc agaccgcgcc gcgcagctgg
     6301 tcgagcggct tgcgggctcg cgatcgcacg tgccccgtga gccggaggga gccggatgcc
     6361 actgaccgaa ctgcgcggac tcgacgagcg cctcgtggtc gacacgtccg aggcgcgccc
     6421 ggtgttcctc ctggccggat ccaacggcgg ctacttgcgg atctccgagg cgactcgcca
     6481 cttcctccag cgggttcagc agggtacttc gttcgaggag ctcgcgcggt ccatgcgccg
     6541 gcaagacggt tcgccgttgt cggcggagga ggtcgaatcc aagtaccgcc agctcacgga
     6601 tcagatcgcc cggttcgagg agggcgggag cgaacccaag ggggccttct ggttccgccg
     6661 caggctggtg cggggcgcga cgatcgccag gctggcggcg ctatgctccg ccgcgctccg
     6721 cccggccgtg gccgtggtcc tggtcgccgc gatcgtcgcc gcggtcgtgg ctgcgacccg
     6781 gctggtgacc ccgacagcgc ccaagcagtt ctggctcagc tacgcggtct tcctcgtgtc
     6841 cctgctggtg cacgaactcg gccacgcgag cgcgtgcgta cggttcggtg gcaaacccag
     6901 cgacatcggg atcgtgatct acctcctcta tccgtcgttc tacagcgacg tcaccgtcgc
     6961 gtgggagttc cggcgccacc agcgcgtggt ggtggacctg gccggcgtgt acttccagct
     7021 aatcttcggc gcaggctgtg tcgcagtgta tgcagtcaca ggatgggagc ccctcgaggg
     7081 tgcgctgctg ttcatcatgg gcagttgcct gttctcgctc aatcccatcc tcaagttcga
     7141 cggctactgg gtggtcgccg acgccctcgg ggtcaccaac ctgggccggc agcggatgcg
     7201 gctcacgcgc cacctcctcg ccctcgcgcg cggtcgcgcg gtccgggccc tgccgtggac
     7261 gcggttcgtc agcatcgcgc tcgcgctcta caccgtggtc gcgctcggct tctgggtgtg
     7321 gttcctgtgg cggctgtgcc cgacgatcgg ccaccagatc gtccgctggg gggagttcgc
     7381 cgcggcgttc gggcgagagc tgatcttcgc ccagtccgga cttgcgtggg agacgacgaa
     7441 gaacctgctg acctcgacgt tcgtgctgct gttcgtggtg ttcatgacgg gccggttact
     7501 cgcgccggcg gtccggtggg tacggctgcg gctcgggacc ggtcggtcgc cagcatgacc
     7561 ctagccaagg agcgtactgt ggtggttgcc gatctcgcgt cagcgggttc cgcggtcgag
     7621 cgcgcgctgg cctttgtggt ccgtggccag ctcgcgaccg g
//
LOCUS       DASVOR010000591         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_268348,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000591 DASVOR010000000
VERSION     DASVOR010000591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268348"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VF516_11675"
     CDS             <1..310
                     /locus_tag="VF516_11675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108382.1"
                     /translation="QTPDLKGAFRTGMLRQIEMTGPYMHTGSLETLEDVVRFYNWGGG
                     DANFAGTKSPAMVPLLLTDQEQSDLVAFLHSLTGDPPPSELGKNTALPDPVPPVPPVQ
                     "
     gene            complement(385..1344)
                     /locus_tag="VF516_11680"
     CDS             complement(385..1344)
                     /locus_tag="VF516_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108383.1"
                     /translation="MKYIYTIALISLAACTAKPGDHELATSIAKGLVAACPAGADPAD
                     EVARNDCAGKLTNLAVLRDAMREPFIWGGQQPGKGYALETKTNKFNARVWRRMYLSTF
                     MFGSNYSIEQVADTTVLHVPISFRGAMSTGAYPYPFWHSEKKWNAYNFATTIHFIIQN
                     GAVIGALRSTEQDTTRPKVAHSWDGLWQWKQDGVQMPYVSFYDYLLSKGNPHARELND
                     AYRALELRMRQNACQACHAPDDQGKSDQLEFFVYPNQALTGRHDLVAQLTDNMMPPED
                     NTLKLPAGIADPAERAALLELARDFERAGDQALAWEGENKPES"
BASE COUNT          227 a          528 c          498 g          256 t
ORIGIN      
        1 gcagaccccc gatctgaagg gcgcgttccg caccggcatg ctgcgccaga tcgagatgac
       61 cggaccgtac atgcacaccg gcagcctgga gaccctggag gacgtcgtgc ggttctacaa
      121 ctggggcggt ggcgatgcca acttcgcagg gaccaagagc ccggccatgg tgccgctcct
      181 gctgaccgac caggagcagt cggatctcgt cgccttcctg cactccctca ccggcgaccc
      241 gccgccgtcc gagctcggca agaacaccgc actcccggat ccggtgcccc cggtgccgcc
      301 cgtgcagtga atccagccgg ccggcggtcc aggatcacca gcgccgtgat gcgaggcgcg
      361 cgcctactcc gacgggacgg ccccctagga ctcgggcttg ttctcgcctt cccaggccag
      421 cgcctggtcg ccggctctct cgaagtcgcg cgcgagctcg agcagcgccg cgcgctccgc
      481 cggatccgcg atcccggccg gcagcttcag ggtgttgtcc tccggcggca tcatgttatc
      541 cgtcagctgt gcgacgaggt cgtggcggcc ggtcagcgcc tggttgggat agacgaagaa
      601 ctcgagctga tccgatttgc cctgatcgtc gggcgcgtgg caggcctggc aggcgttctg
      661 ccgcatccgc agctccagcg cgcggtacgc gtcgttgagc tcgcgggcgt gcgggttccc
      721 cttcgacagc aggtagtcgt agaacgacac gtatggcatc tgcacgccat cctgcttcca
      781 ctgccacagg ccgtcccacg agtgcgcgac cttgggccgc gtggtgtcct gctccgtgga
      841 tcgcagcgcg ccgatcaccg caccgttctg gatgatgaag tggatcgtcg tcgcaaagtt
      901 gtaggcgttc cacttcttct cgctgtgcca gaacggatac ggatacgcgc cggtcgacat
      961 cgcgccgcga aagctgatcg ggacgtggag caccgtggtg tcggcgacct gctcgatcga
     1021 gtagttgctg ccgaacatga acgtcgacag gtacatccgg cgccacaccc gggcgttgaa
     1081 cttgttggtc ttggtttcga gcgcgtagcc cttgcccggt tgctgaccgc cccagatgaa
     1141 cggctcgcgc atcgcgtcgc gcagcacggc gaggttggtg agcttgcccg cgcagtcgtt
     1201 gcgcgccacc tcgtcggccg ggtcggcgcc cgccgggcac gccgccacca ggcccttcgc
     1261 gatcgacgtc gcgagctcgt ggtcccccgg cttggccgtg cacgcggcca gcgagatcag
     1321 ggcgatcgta tagatgtact tcatgtaagc cctcacttcg tgaacagcgt gaccagggtg
     1381 agcgcgccgg cgtcgacggt gaacgtgccg ggccagatga cctcgtcgac cgcgtacggc
     1441 ggcgcgacct ccgccacccc gcgcagcgcc agctgcatga tcgacacgtg cggctgcacc
     1501 ccgagcgcc
//
LOCUS       DASVOR010000592         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_385312,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000592 DASVOR010000000
VERSION     DASVOR010000592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385312"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VF516_11685"
     CDS             complement(<1..524)
                     /locus_tag="VF516_11685"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8108384.1"
                     /translation="MTKGEETRQRILDRAFRIAGRDGLDGLTLNTLATDLGMSKSGLF
                     AHFRSKEELQLQVLETAAARFAARVIAPALRAPRGVPRLKKLFDAWLHWITDPAQPGG
                     CIFVAAATELDDKDGRPRELLVDGQRRLRDTLARLARAAIDERQLRRDLDCEQLAFEL
                     FAIILAYHHDKRLLR"
     gene            complement(607..1329)
                     /locus_tag="VF516_11690"
     CDS             complement(607..1329)
                     /locus_tag="VF516_11690"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX8108385.1"
                     /translation="MLPIRAPAHTRDRGHRCDGAAALVFCAGFAYECGMPHTWDPLSL
                     SPEAALERLVEGNQRFRRGDGAVAGRGFDPARAAGTQRPFAIVLGCSDSRTPVEIVFD
                     QGFGDLFVVRVAGNIVAPSIVGSIEFAASQFGTRLVVVMGHTGCGAIAATLHALATGM
                     GPASKNLRAITDRIAPHIEAIACGAGPDTSREAMRANVRASVDHLRHGSQILEELVLK
                     QRVRVVGAEYELETGQVVFFDH"
BASE COUNT          234 a          570 c          564 g          201 t
ORIGIN      
        1 cggagcagcc gcttgtcgtg gtggtaggcc aggatgatcg cgaacagctc gaacgcgagc
       61 tgctcgcagt cgaggtcgcg gcggagctgg cgctcgtcga tcgcggccct ggcgagccgc
      121 gcgagggtgt cgcgcagccg gcgctgacca tcgaccagga gctcgcgcgg cctgccgtcc
      181 ttgtcgtcca gctcggtcgc cgcggcgacg aagatgcagc cgccgggctg cgcgggatcg
      241 gtgatccagt gcagccaggc gtcgaacagc ttcttgagcc gcggcacgcc gcgcggggcg
      301 cgcagcgccg gtgcgatcac gcgggccgcg aaccgcgccg ccgcggtctc caggacctgg
      361 agctggagct cctccttgga gcggaagtgg gcgaacagcc cgctcttgga catccccagg
      421 tcggtggcga gggtgttcag ggtcagcccg tccagaccgt cgcgccccgc gatgcggaac
      481 gcccggtcga ggatgcgctg ccgggtctcc tcacctttcg tcacgtataa aaagtacgac
      541 cggtcgtttc tttgtcaagc ccccctccga ccggcgggcc cgctgctcac cgcgaaccac
      601 cgccgatcag tgatcgaaga acacgacctg gccggtctcc agctcgtact cggcgccgac
      661 cacgcggacg cgctgcttga gcaccagctc ctccaggatc tggctgccgt gccgcaggtg
      721 gtcgaccgac gcccgcacgt tggcgcgcat cgcctcacgc gaggtgtccg ggccggcgcc
      781 gcacgcgatc gcctcgatgt ggggcgcgat ccggtccgtg atcgcgcgca ggttcttcga
      841 cgcggggccc atgccggtgg cgagcgcgtg gagggtcgcg gcgatcgcgc cgcagccggt
      901 gtgccccatc acgacgacca gccgggtgcc gaactgcgac gccgcgaact cgatcgagcc
      961 gacgatcgac ggcgcgacga tgttgccggc gacccgcacg acgaacaggt cgccgaaccc
     1021 ctggtcgaac acgatctcga ccggcgtccg cgagtccgag cagccgagca cgatcgcgaa
     1081 cggccgctgg gtccccgccg cgcgcgccgg atcgaacccg cgcccggcga ccgcgccatc
     1141 cccgcggcgg aaccgctggt tgccttcgac gaggcgctcc agcgcggcct cgggcgacag
     1201 cgacagcggg tcccaggtat gcggcatgcc gcactcatac gcgaagccgg cgcagaacac
     1261 cagcgccgcg gcgccgtcgc agcgatggcc gcgatctcgc gtgtgcgcgg gcgctcggat
     1321 agggagcacg gcgagctcga gctcctggag tcgcgccggg tcgccaggat ggcgctctcg
     1381 atgacccggt cgtcgccgat cgggaagcgc agcacgcgga gcagccgccc gcgccgcgcc
     1441 aggaccaggc catcgcgcgg atcaacgcct tgaaccgggt caacgtcacg agccggcaga
     1501 tcgcgctcgg cccacgcgga tccgcggcga gccggcgcgg gccgcgcgtg gtagcaagca
     1561 gacatgacg
//
LOCUS       DASVOR010000593         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_210177,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000593 DASVOR010000000
VERSION     DASVOR010000593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210177"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VF516_11695"
     CDS             <1..495
                     /locus_tag="VF516_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108386.1"
                     /translation="GGEVYTWRADGTLGLLARAPKPLEEFGEAGPDHIIAIAGDTTIY
                     SMALTPGPRAAGDASEALASIASNTAAMAPDTGMLVAIERGALTILDPLVHQRWTLAP
                     PGGATFVAPAISADGRRVIAGTPHSLLAWSIDLPDSAEATARWLDAMTNAVDDRTPGG
                     LGWR"
     gene            546..>1789
                     /gene="cysS"
                     /locus_tag="VF516_11700"
     CDS             546..>1789
                     /gene="cysS"
                     /locus_tag="VF516_11700"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEX8108387.1"
                     /translation="MTPVRLYDSFRREKVPFEPLQPGKVSMYVCGPTPYAPAHIGHAY
                     SAIAFDMIRRSLRFLGYEVRYVRNVTDVEDKIIKRANENGEDPMALAARFAADYNRDM
                     AGFGVLPPDVEPKVSTHISEIIALIERLVERGSAYPVDGDVYFEVATFPAYGALSGMK
                     RDELRAGERVEIDERKRDPADFALWKAAKPGEPSWDSPWGKGRPGWHIECSAMTHTHL
                     GDTFDLHGGGKDLIFPHHENEIAQSRGAYGPATFARYWTHNGFLNFKGEKMSKSLGNV
                     IDCSVIGDEVGGEALRLFCVSHHFRSPVDFDFEAVRDHAGHVLGARFHSLESADKKLD
                     YFYTTLKRVDDFVAQGGDAADGKVLPEAEKLVPDARAALADDFNAPLVVAALYEAAAL
                     ANRLLDEPRGIDKAVRRRTLARL"
BASE COUNT          277 a          677 c          599 g          236 t
ORIGIN      
        1 ggcggcgagg tctacacctg gcgcgccgac ggtacgctcg gcctgctcgc ccgcgcgccc
       61 aagccgctcg aggagttcgg cgaggccggc cccgaccaca tcatcgcgat cgccggcgac
      121 acgacgatct attcgatggc cctcacgccc ggcccacgtg cggccggcga cgccagcgag
      181 gcgctcgcca gcatcgcgag caacacggcg gcgatggccc ccgacaccgg gatgctggtc
      241 gcgatcgagc gcggcgcact cacgatcctc gatccgctgg tccaccagcg ctggacgctc
      301 gccccgcccg gcggcgcgac gttcgtggca cccgcgatct cggccgacgg ccgccgcgtc
      361 atcgccggca ccccgcacag cctgctggcg tggtcgatcg acctgccgga cagcgccgag
      421 gccaccgcgc gctggctcga cgcgatgacc aacgccgtcg acgaccggac cccgggcggc
      481 ctcggctggc ggtagcgccc cgcgcccggt gggtcggccc ggccagtagt gctacaaccg
      541 accgggtgac cccggtccgg ctctacgatt cgttccgacg cgagaaggtc ccgttcgaac
      601 cgctccagcc gggcaaggtc agcatgtacg tgtgtgggcc gacgccctac gccccggccc
      661 acatcggcca cgcctactcc gcgatcgcgt tcgacatgat ccggcgcagc ctgcggttcc
      721 tcggctacga ggtgcgctac gtgcgcaacg tcaccgacgt cgaggacaag atcatcaagc
      781 gcgccaacga gaacggcgag gacccgatgg cgctcgccgc ccggttcgcc gccgactaca
      841 accgcgacat ggcggggttc ggcgtgctgc cgcccgacgt cgagcccaag gtctcgaccc
      901 acatctccga gatcatcgcg ctgatcgagc gcctggtcga gcgcggctcg gcctacccgg
      961 tcgacggcga cgtgtacttc gaggtcgcga ccttccccgc ctacggcgcg ctgtccggca
     1021 tgaagcgcga cgagctgcgc gccggcgagc gggtcgagat cgacgagcgc aagcgcgatc
     1081 cggccgactt cgcgctgtgg aaggcggcca agcccggcga accatcctgg gactcgccgt
     1141 ggggcaaggg ccgaccgggc tggcacatcg agtgctcggc gatgacccac acccacctcg
     1201 gcgacacctt cgacctccac ggcggcggca aggatctgat cttcccgcat cacgagaacg
     1261 agatcgccca gtcgcgcggc gcctacggcc cggccacgtt cgcgcgctac tggacgcaca
     1321 acggcttcct caacttcaag ggcgagaaga tgagcaagtc gctgggcaac gtgatcgact
     1381 gcagcgtgat cggcgacgag gtcggcggcg aggcgctgcg gctgttctgc gtctcgcacc
     1441 acttccgctc gccggtcgac ttcgacttcg aggcggtgcg cgaccacgcc ggccacgtcc
     1501 tcggcgcgcg gtttcacagc ctggagtccg ccgacaagaa gctcgactac ttctacacca
     1561 cgctcaagcg ggtcgacgac ttcgtcgccc agggcggcga tgccgccgac ggcaaggtgc
     1621 tgcccgaggc cgagaagctc gtgcccgacg cgcgcgccgc gctggccgac gacttcaacg
     1681 cgccgctggt ggtcgccgcg ctgtacgagg ccgcggcgct cgccaatcgc ctgctcgacg
     1741 agcccagggg catcgacaag gcggtgcgcc gccgcaccct ggcacggct
//
LOCUS       DASVOR010000594         6304 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_34653,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000594 DASVOR010000000
VERSION     DASVOR010000594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6304
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34653"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VF516_11705"
     CDS             complement(<1..371)
                     /locus_tag="VF516_11705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108388.1"
                     /translation="MRSGGASVDGTTLLYIGVRPGVDGHVLRSVCRAVVLLLPACGGG
                     GGGDAGTGFCYGPSGWQVCLGAKATGQVQLQGTLDTGKSDASNPCLNSQPTGWTATQP
                     DACIIVGDTIAVTSLAVTGSRP"
     gene            complement(549..1469)
                     /locus_tag="VF516_11710"
     CDS             complement(549..1469)
                     /locus_tag="VF516_11710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108389.1"
                     /translation="MRTHPLLVIAACALAGVTAVTAVASAQPAQKITIGIYAPSVEFG
                     AAQARLAYVQDLARAIAQATGLTTEAQSYANIAALKKDAVDFAIIDGVCFAANLNWKL
                     LATANIGGGTTRPWALYSSVGESMQALKGKKLAFIGTGCNDAGFVDNAMLESEVDPAF
                     FGARSAKPDLTAAVAEVASYKAAQAVFAPAGAAKGLTKVFDTGAVPNPAFVEVGGKLP
                     PAIVDRVAASVIGYGGSGAIAGWTRPSREIYTALATRMGKVVKTGVFASADPVRIDAR
                     DALIEPPTLRDTAVVDVRHHFVRPSSGRME"
     gene            complement(1480..4047)
                     /locus_tag="VF516_11715"
     CDS             complement(1480..4047)
                     /locus_tag="VF516_11715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8108390.1"
                     /translation="MRPGALVLALAAAGLGLCALGALHPRTAAAAVEDDLRDGDRYFE
                     ESDWHKAATAYDRAIAKAPSEVSAEAYGKRAAIFIILKDYKGGLDFIERAKARYPSAP
                     EVLEQEALILWETDRKDEAIKVAEAVVATRPQAFTNQKLIGEYYAPRDPARTIAAYEH
                     YLANRPADLEAGDVLPRVRLGFAYLATARLAIADGDDARAGQLFGKAADQFDLVQRKF
                     GKKPNAEINADNGLCAALTGLGRFDQAVTVCERVVDDPRRIDSTGSAWFNLATAYLAR
                     KQTKKARTAGTEFTRLRKTEARGYILLGDTYFADRDWAGALDQYLKAEKLLKANQAHD
                     QVQLSIKLGKTYRRLPSASMGPGGGPGGNLAIAIEKLSSGFSANPGSLELALELGGAY
                     LEAHQDARAIALTDRMLGGADGAKLPAEARGNLLVLAGKAQFNQHKLKEARQRFEAAR
                     ELRPADVTVQRGLVLTINEQAFEADKDPRVAQVLLEQALAIDPQSPVTITNLAVLAID
                     RGECDAARQQLAKLDSIRGHDTVARARLLARSYLRGARPDPRRAAEAYAVAEREAKKA
                     SAQLALAEIYTEWAPLIWDTDLADAVEKLETAVGSSTGDPAIASAAKRNLAIALFRRG
                     WRSMRDGKAGEAAADLEKATRDPGVLKGSEPAAFEFSYALALLDSGRAPEAAKLFRGL
                     AAKGGQTSYLKGGYARFGTQLFAAYANYRNGSPAARQQAAAEFAKLLNEPGLGDKVKE
                     LLAACWEAIAIDQWRSGQLAAAGRSLTTAEKYATGDIKRRVTMDRAALSLGKTNLGAM
                     EGLAGNPPESLVNLGIIYDQLGRPREAFDAWTKAKARGVQVRDLQRWIDAKKRIYGF"
     gene            complement(4044..5756)
                     /locus_tag="VF516_11720"
     CDS             complement(4044..5756)
                     /locus_tag="VF516_11720"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8108391.1"
                     /translation="MAARGGLSLSVKMILTTTLLIVVTVVGSGVLNVINIRRAFDESA
                     RQQIDVFRAGREAAGEFGTPLFARAVEQLLIDRGRDVELRSLVKETVALDTRDGPSGK
                     RDYGLRLAYVLDLNRQVVAHCFEDPKLTCFEGQGHAPIGPALGPLTVDTWNDALAEWK
                     KAAAGRGEPLIKFDKSAGDSTYRVFASPVFEGIPATAAGALPVEPPPHREGYVVLGYD
                     LAPIDWFAQAAAEQKANASTRAALYTGAVGALFALIGTVLAILQGLSISRPIKQLAWR
                     ADQIARGDLGARVEITSSDEIGLLGENFNFMADQISILLEQTAEKARIEQELEIAKTI
                     QDTLVPSAEPVDQGALRFAGFYQPAAQTGGDWWTWHELVGGKVLLVVGDVTGHGVPSA
                     MITAAAKAACDVARYVYRDDVTVSGLLEIMNHAIFESAQRRFVMTCFASIFDPQRRTI
                     TYANAGHNFPYLFRPGDGRGEFGSLMIRGNRLGDDRNSRYEAKTTELAPGDVLIWYTD
                     GIVECENASGEEYGEKRFRASVKRAAALDAGEMRDAIVGDATAFFGDTPRKDDITMIV
                     GRIH"
     gene            5804..>6304
                     /locus_tag="VF516_11725"
     CDS             5804..>6304
                     /locus_tag="VF516_11725"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8108392.1"
                     /translation="MSVEPIPPALQQFLTEFKRVASAIVDSYFVVDTERRIVDFNRAF
                     FALLPRQVARGLKGKRCYEVIELNICKTECIAQQCWADNRHVRLDEISGSVVGEAQKL
                     RFILSAVPITDERGNHVGALEIQRNVTDEAVVQVKYQEMLETEARERERLANQVRMRT
                     KELLETN"
BASE COUNT          853 a         2178 c         2300 g          973 t
ORIGIN      
        1 gggcgagatc ccgtgacagc cagcgacgtc acggcgatgg tgtcaccgac gatgatgcac
       61 gcatccggct gagtggcagt ccagcccgtc ggctggctgt tcaggcacgg attgctggcg
      121 tcgctcttgc cggtatcgag cgtgccctgg agctggacct gcccggtcgc cttggctccc
      181 agacacacct gccagccgct cggcccgtag caaaagccgg tccctgcatc gccacctccg
      241 ccaccaccgc acgcgggaag aagcagaacc accgcacgac aaaccgatcg cagcacgtgt
      301 ccatctacac ccggccgaac gccgatgtac agcagcgttg tgccgtcaac ggatgcgcct
      361 ccggagcgca tgcgctagcg atgacaagat gccgtgtagc tggatccgcg ccccccgctg
      421 acccgctgcc tcaaagggtg acggctgcct caaaaggccg atctccgacg gggttttgag
      481 gcaaagcccc ggtaccgccg ccgctggccg gtgccgcttc cgctgccgct cgccggtgcc
      541 gctgaccgct actccatccg tccgctggac ggccgcacga agtggtggcg gacgtcgacc
      601 accgcggtgt cgcgcagggt cggcggctcg atcagcgcgt ccctggcgtc gatccggacc
      661 ggatcggcgc tggcgaacac gccggtcttc accaccttgc ccatccgggt ggcgagcgcg
      721 gtgtagatct cgcgcgatgg ccgggtccag cccgcgatcg cgccggagcc gccgtagccg
      781 atcaccgacg cggcgacgcg gtcgacgatg gcaggcggca gcttgccgcc gacctcgacg
      841 aacgccgggt tgggcacggc gccggtgtcg aacaccttgg tgaggccctt ggccgcgccg
      901 gccggcgcga acaccgcctg ggcggccttg tacgacgcga cctcggcgac cgcggcggtg
      961 agatccggct tggcgctgcg cgcgccgaag aacgccggat cgacctcgga ctcgagcatc
     1021 gcgttgtcga cgaaaccggc gtcgttgcag ccggtgccga tgaacgcgag cttcttgccc
     1081 ttgagcgcct gcatcgactc gccgacgctg gagtacagcg cccatggccg ggtcgtgcca
     1141 ccgccgatgt tcgcggtggc gagcagcttc cagttgaggt tggccgcaaa gcacacgccg
     1201 tcgatgatcg cgaagtcgac cgcgtccttc ttgagcgcgg cgatgttggc gtacgactgg
     1261 gcctcggtcg tcaggccggt ggcctgcgcg atcgcgcggg cgagatcctg gacgtaggcg
     1321 aggcgggcct gggcagcgcc gaactcgacc gacggggcgt agatgccgat cgtgatcttc
     1381 tgcgccggct gggcggacgc gacggccgtg acggcggtga cgccggcgag cgcgcatgca
     1441 gcgatgacga ggagggggtg ggtgcgcatg gtgatcgccc tagaacccat agatccgctt
     1501 cttggcatcg atccagcgct gcaggtcgcg gacctggacg ccgcgtgcct tggccttggt
     1561 ccaggcgtcg aacgcctcgc gcggccggcc gagctggtcg tagatgatgc cgaggttgac
     1621 gagcgactcg ggcgggttgc cggcgaggcc ctccatcgcg ccgaggttgg tcttgccgag
     1681 cgacagcgcg gcgcggtcca tcgtgacccg gcgcttgatg tcgccggtgg cgtacttctc
     1741 ggcggtggtg agcgaccggc cggcggcggc gagctggccc gagcgccact ggtcgatcgc
     1801 gatcgcctcc cagcacgccg cgagcagctc cttgaccttg tcgccgaggc cgggctcgtt
     1861 gagcagcttg gcgaactcgg cggcggcctg ctggcgggcg gccggcgacc cgttgcggta
     1921 gttggcgtag gcggcgaaca gctgggtgcc gaaccgggcg tagccgccct tgaggtagct
     1981 ggtctggccg cccttggcgg cgaggccgcg gaacagcttg gcggcctccg gggcgcggcc
     2041 gctgtcgagc agggcgagcg cgtacgagaa ctcgaacgcg gccggctcgc tgcccttgag
     2101 cacgccgggg tcgcgggtcg ccttctcgag gtcagcggcg gcctcgccgg ccttgccgtc
     2161 gcgcatcgac cgccagccgc ggcggaacag cgcgatcgcg aggttgcgct tggcggccga
     2221 ggcgatcgcg ggatcgccgg tcgagcttcc gaccgcggtc tcgagcttct cgacggcgtc
     2281 ggccaggtcg gtgtcccaga tcagcggcgc ccactcggtg tagatctcgg cgagcgcgag
     2341 ctgggcgctg gccttcttgg cctcgcgctc ggcgaccgcg taggcctcgg cggcgcgccg
     2401 cgggtcgggc cgggcgccgc gcagatagct gcgcgccagc agccgggcgc gcgcgacggt
     2461 gtcgtggccg cggatcgagt cgagcttggc gagctgctgg cgggcggcgt cgcactcgcc
     2521 gcggtcgatc gcgagcaccg cgaggttggt gatcgtcacc ggcgactgcg ggtcgatcgc
     2581 gagcgcctgc tcgagcagca cctgggccac gcgcggatcc ttgtcggcct cgaacgcctg
     2641 ctcgttgatc gtcagcacca ggccgcgctg gacggtgacg tcggccgggc ggagctcgcg
     2701 cgcggcctcg aaccgctggc gcgcctcctt gagcttgtgc tggttgaact gggccttgcc
     2761 ggcgagcacg agcaggttgc cgcgggcctc ggcggggagc ttggcgccgt cggcgccgcc
     2821 gagcatgcgg tcggtgagcg cgatcgccct ggcgtcctgg tgggcctcga ggtaggcgcc
     2881 gccgagctcg agcgcgagct cgaggctgcc cgggttggcg gagaacccgc tcgacagctt
     2941 ctcgatcgcg atcgcgaggt tgccgcccgg gccgccgcca gggcccatgc tagccgacgg
     3001 cagccggcgg taggtcttgc ccagcttgat cgagagctgg acctggtcgt gggcctggtt
     3061 ggccttgagc agcttctcgg ccttgaggta ctggtccagc gccccggccc agtcgcggtc
     3121 ggcgaagtag gtgtcgccga gcaggatgta gccgcgcgcc tcggtcttgc gcagccgggt
     3181 gaactcggtg cccgcggtgc gcgccttctt ggtctgcttg cgcgcgaggt aggcggtcgc
     3241 gaggttgaac cacgccgagc cggtgctgtc gatccgccgc ggatcgtcga ccacgcgctc
     3301 gcacaccgtg accgcctgat cgaaccggcc caggccggtc agcgcggcgc acaggccgtt
     3361 gtcggcgttg atctcggcgt tgggcttctt gccgaacttg cgctgcacga ggtcgaactg
     3421 gtcggccgcc ttgccgaaca gctgcccggc cctggcgtcg tcgccgtcgg cgatcgcgag
     3481 ccgcgcggtc gccaggtacg cgaagccgag gcggacccgc ggcagcacgt cgccggcctc
     3541 gaggtcggcc gggcggttgg cgaggtagtg ctcgtaggcg gcgatcgtcc tggccggatc
     3601 gcgcggcgcg tagtactcgc cgatcagctt ctggttggtg aacgcctggg gccgggtcgc
     3661 gaccacggcc tcggcgacct tgatcgcctc gtccttgcgg tcggtctccc acaggatcag
     3721 ggcctcctgc tcgaggacct cgggcgcgct cgggtagcgc gccttggccc gctcgatgaa
     3781 gtccaggccg cccttgtagt ccttgaggat gatgaagatc gcggcgcgct tgccgtaggc
     3841 ctcggccgac acctcgctgg gcgccttggc gatcgcgcgg tcgtacgcgg tcgccgcctt
     3901 gtgccagtcg ctctcctcga agtagcggtc gccgtcgcgc agatcgtcct cgaccgccgc
     3961 ggcggcggtc cgcgggtgca gggcgcccag ggcgcaaagc ccgagccccg cggcggcgag
     4021 cgcgagcacc agggcaccgg gtctcaatgg atcctcccca cgatcatggt gatgtcgtcc
     4081 ttgcgcggcg tgtcgccgaa gaacgcggtc gcgtcgccga cgatcgcgtc gcgcatctca
     4141 ccggcgtcga gcgcggcggc gcgcttgacc gaggcccgga accgcttctc gccgtactcc
     4201 tcgccactgg cgttctcgca ctcgacgata ccgtcggtgt accagatcag cacgtcgccc
     4261 ggggccagct cggtggtctt ggcctcgtag cgcgagttgc ggtcgtcgcc gagccggttg
     4321 ccgcggatca tcagcgagcc gaactcgccg cggccgtcgc cggggcggaa caggtacggg
     4381 aagttgtggc cggcgttggc atacgtgatc gtgcggcgct gcgggtcgaa gatcgacgcg
     4441 aagcaggtca tcacgaaccg gcgctgcgcg ctctcgaaga tcgcgtggtt catgatctcg
     4501 agcagccccg acaccgtgac gtcgtcgcgg tagacgtagc gcgcgacgtc gcacgccgcc
     4561 ttggccgccg cggtgatcat cgccgacggg acgccgtggc cggtgacgtc gccgaccacc
     4621 agcagcacct tgccgccgac cagctcgtgc caggtccacc agtcgccgcc ggtctgcgcc
     4681 gccggctggt agaacccggc gaaccggagc gcaccttggt cgaccggctc ggcgctcggc
     4741 accagggtgt cctggatcgt cttggcgatc tcgagctcct gctcgatccg cgccttctcg
     4801 gcggtctgct cgagcaggat cgagatctgg tcggccatga agttgaagtt ctcgccgagc
     4861 aggccgatct cgtcggacga cgtgatctcg acccgggcgc cgaggtcgcc gcgcgcgatc
     4921 tggtcggcgc gccaggcgag ctgcttgatc ggccgcgaga tcgacaggcc ctgcaggatc
     4981 gccagcacgg tgccgatcag cgcgaacagc gcgccgaccg cgccggtgta cagcgccgcc
     5041 cgggtcgacg cgttcgcctt ctgctcggcc gcggcctggg cgaaccagtc gatcggcgcg
     5101 aggtcgtaac cgagcacgac gtagccctcg cggtgcggcg gcggctcgac cggcagcgcg
     5161 cccgccgcgg tggccgggat gccctcgaac accggggagg cgaacacgcg gtaggtcgag
     5221 tccccggcgg acttgtcgaa cttgatcagc ggctcgcccc ggccggccgc ggccttcttc
     5281 cactcggcga gcgcgtcgtt ccaggtgtcg acggtgagcg ggcccagggc cgggccgatc
     5341 ggcgcgtggc cctggccctc gaagcaggtc agcttggggt cctcgaagca gtgcgcgacg
     5401 acctggcggt tgaggtcgag cacgtaggcc aggcgcaggc cgtagtcgcg cttgccgctc
     5461 ggcccgtccc gggtgtcgag cgcgacggtc tccttgacca gcgagcggag ctcgacgtcg
     5521 cggccgcggt cgatcaggag ctgctcgacg gcgcgcgcga acagcggcgt gccgaactcg
     5581 ccggcggcct cgcggccggc ccggaacacg tcgatctgct ggcgcgccga ttcgtcgaac
     5641 gcccgccgga tgttgatcac gttgagcacg ccggagccga ccaccgtgac gacgatcagg
     5701 agcgtcgtcg tcaggatcat cttgaccgag aggctcagac ccccgcgagc tgccatcccg
     5761 agcgaggata tgccagccgc gggcgctctg gtaggatttg tcggtgtctg tcgagcccat
     5821 cccccccgcg ctgcagcagt tcctgaccga gttcaagcgg gtggccagcg cgatcgtcga
     5881 cagctacttc gtcgtcgaca ccgagcgccg gatcgtcgac ttcaaccgcg cgttcttcgc
     5941 cctcttgccc cgccaggtgg cgcgcggcct caagggcaag cggtgctacg aggtgatcga
     6001 gctcaacatc tgcaagaccg agtgcatcgc ccagcagtgc tgggccgaca accggcacgt
     6061 ccggctcgac gagatctccg gctcggtggt cggcgaggcc cagaagctcc ggttcatcct
     6121 gtccgcggtg ccgatcaccg acgagcgcgg caaccacgtc ggggcgctcg agatccagcg
     6181 caacgtcacc gacgaggcgg tcgtccaggt caagtaccag gagatgctgg agaccgaggc
     6241 ccgggagcgc gagcggctcg ccaaccaggt ccggatgcgg accaaggagc tgctcgagac
     6301 caac
//
LOCUS       DASVOR010000595         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_327047,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000595 DASVOR010000000
VERSION     DASVOR010000595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_327047"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VF516_11730"
     CDS             <1..1263
                     /locus_tag="VF516_11730"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX8108393.1"
                     /translation="RIARPIHGEPDLDRLLDQIAAARVVLIGEASHGAHELNDLRGTL
                     TRKLIAERGFAAVAIAGGWPEAIRVDRYVRGQGHDESAAAALVGFERFPPWIWRNADV
                     TAFADWLATWNAQRPADQRAGFYGLDLYSMYVAIRSLLSFLDDADPAAGHAARALYTG
                     LDQVAGAGEDRPGLEVAAGYEDEVVAQLVEMQRRHAARSGRTPAGEGWFHAMQSAHVL
                     QNAAAYYRTLLRGRVAWNARDARMADALDMLADQLGQPGKPARLVVWAHNRHVGDARA
                     TAMGEAGEISLGQMMRQRHPGETALVGMIAYGGTVTCARGWDEPAEVATVPPGLDGSW
                     EQLLHACDLPRLYLGTASLRREVGEGAERLQRAIGVVYRPETERLRHYHLSRLADEFD
                     VVIHVDAVRAATGVDVVEGPLEPAGAVG"
     gene            1279..>1532
                     /locus_tag="VF516_11735"
     CDS             1279..>1532
                     /locus_tag="VF516_11735"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8108394.1"
                     /translation="MTIFTAKEYLALEAVAEIRHEFSGGQIVAMAGAELEHTQIAQNV
                     RSELTAALGDRPCHIVGSDQRVHVEATGEYFYPDVLVTCL"
BASE COUNT          203 a          551 c          585 g          193 t
ORIGIN      
        1 cggatcgcgc ggccgatcca cggtgagccg gatctcgacc ggctgctcga ccagatcgcg
       61 gcggcgcgcg tcgtgctgat cggcgaggcg agccacggcg cgcacgagct caacgatctg
      121 cggggcacat tgacccgcaa gctgatcgcc gagcgcgggt tcgccgcggt ggcgatcgcg
      181 gggggctggc cggaggcgat ccgggtcgac cgctacgtgc gcggccaggg ccacgacgag
      241 tcggcggccg ccgcgctcgt cggcttcgag cggttcccgc cctggatctg gcgcaacgcc
      301 gacgtcaccg cgttcgccga ctggctcgcg acgtggaacg cgcagcggcc ggccgaccag
      361 cgcgccgggt tctacggcct ggacctgtac tcgatgtacg tcgcgatccg ctcgctgctg
      421 tcgttcctcg acgacgccga cccggcggcc gggcacgccg cccgcgcgct ctacaccggg
      481 ctcgatcagg tcgccggggc cggcgaggac cggccgggcc tcgaggtcgc ggcgggctac
      541 gaggacgagg tcgtcgccca gctggtcgag atgcagcgcc gccacgccgc gcgctcgggg
      601 cggacgcccg ccggcgaggg ctggtttcac gcgatgcaat cggcgcacgt gctgcagaac
      661 gccgcggcgt actaccgcac gctgctgcgc ggccgggtgg cgtggaacgc gcgcgacgcc
      721 cggatggccg acgcgctcga catgctcgcc gaccagctcg ggcagcccgg caagcccgcc
      781 cggctggtcg tgtgggccca caaccgccac gtcggcgacg cgcgcgcgac ggcgatgggc
      841 gaggccggcg agatctcgct cggccagatg atgcgccagc gccaccccgg cgagaccgcg
      901 ctggtcggga tgatcgcgta cggggggacg gtgacctgcg cgcgcggctg ggacgagccg
      961 gccgaggtcg cgacggtgcc gcccggcctc gacggcagct gggagcagct gctgcacgcg
     1021 tgcgacctcc cgcggctcta cctcggcacc gcgtcgctgc gccgggaggt cggcgagggc
     1081 gccgagcggc tgcagcgcgc gatcggcgtg gtgtaccggc ccgagaccga gcggctgcgg
     1141 cactaccacc tgtcgcggct ggccgacgag ttcgacgtcg tgatccacgt cgacgcggtc
     1201 cgcgcggcga ccggggtcga cgtcgtcgag ggcccgctgg agcccgccgg cgcggtgggg
     1261 taggctgccg caacccacgt gacgatcttc accgcgaagg agtacctcgc gctcgaggcg
     1321 gtcgcggaga tccggcacga gttctccggc ggccagatcg tggcgatggc cggcgccgag
     1381 ctcgaacaca cccagatcgc ccagaacgtg cgcagcgagc tgaccgccgc gctcggcgat
     1441 cgaccgtgcc atatcgtcgg cagtgaccag cgggtccatg tagaggcgac cggtgagtat
     1501 ttctatcccg acgtcctggt cacgtgcctc ga
//
LOCUS       DASVOR010000596         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_210192,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000596 DASVOR010000000
VERSION     DASVOR010000596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210192"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2279)
                     /locus_tag="VF516_11740"
     CDS             complement(<1..>2279)
                     /locus_tag="VF516_11740"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX8108395.1"
                     /translation="RSIREYGHLDARLDPLGSPPLGDPMLNAATHGLTDAGLAALPAT
                     IVWPDADSRAGTCLDAIQRLRAIYSGSIGYEFDHVQDFDERTWLYQAVESGAYQPKLT
                     DDGRRDLLRRLSEVEGFERFLHSTFQGQKRFSIEGIDMLVPMLDEAIHEATETGTNEV
                     LIGMAHRGRLNVLAHILGKPYAKIFSEFHSAPNKELVPSEGSTGINYGWTGDVKYHLG
                     ARKLMRNGEAAHVQLTLAHNPSHLEFVNPVIEGFTRAAQEDRAAPGEPHQDADWALAL
                     VIHGDAAFPGEGVVAETLNLSRLPGYQTGGTLHIIANNQIGFTTVPHDGRSTLYASDL
                     AKGFEIPIFHVNADDPEACLTVVGIAEAYRTRFHKDALIDLVGYRRWGHNEGDEPAFT
                     QPVLYASIAARPTVRALYADRLISDGIITPSEVEEMQREVQTRLRAAQEALVSGHAVE
                     QEPAPLHEAPPLTSYTTAVPADSLRALNDALLARPDGYSAHTRLERLLQRRRESIEKP
                     GGIDWGHAETLAFASILADGTPIRLSGQDAERGTFSQRHLVLHDATTGARYVPLQALP
                     QARASFAVYNSPLSEAAVLGFEYGYSVHAPEALVLWEAQFGDFANAGQVLIDQFIAAA
                     RAKWRQHPSLVLLLPHGYEGQGPEHSSGRVERYLQLAAEDNLRVANVTSAAQYFHLLR
                     VQAATLGQAPRPLVIMTPKSLLRQPLAASSLADLASGGFAPVLDDATVADKRDTVRRL
                     VLTSGKVAIDLAAARAAQPG"
BASE COUNT          349 a          663 c          881 g          386 t
ORIGIN      
        1 cctggctgtg cggcgcgagc ggcggccaga tcgattgcca ccttgccgct agtgaggacc
       61 agacgccgca cggtatcgcg cttgtccgcg acggtcgcgt cgtcaaggac gggggcgaag
      121 ccaccggagg cgaggtccgc caggctggag gcggcgagcg gctggcgcag caggctcttg
      181 ggcgtcatga tgacgagcgg acgcggggcc tggccgagtg tcgccgcctg aacacgcagc
      241 aggtggaagt attgcgcggc gctcgtgacg ttggcgacgc gcaggttgtc ttcggcggcg
      301 agctgcaggt agcgctcgac acgcccactc gaatgctcgg gtccctggcc ctcgtagccg
      361 tgcggcagca gcagcaccag cgagggatgt tggcgccact tggcgcgggc ggcggcgatg
      421 aactggtcga tcagcacctg cccggcgttg gcgaagtcgc cgaactgcgc ctcccagagc
      481 accaacgctt cgggggcatg gacgctgtag ccatattcga agccgagcac ggcagcctca
      541 gagagcgggc tgttgtagac ggcgaaggag gcgcgagcct ggggcagcgc ctgcaacggc
      601 acgtagcgcg cgccggtggt cgcatcgtgg agcacgaggt ggcgctggct gaaggtgccg
      661 cgctcggcat cctggccgct gaggcgaatg ggcgtgccat cggcgaggat cgacgcgaag
      721 gcgagtgtct cggcgtggcc ccagtcgatg ccgcccggct tctcgatgga ctcgcggcgt
      781 cgctgcagca agcgctcaag acgagtgtgg gcgctgtagc cgtcgggacg ggccagcagc
      841 gcgtcgttga gcgcgcgcag gctatcggcg ggtacggcgg tggtgtagct ggtgagcggc
      901 ggcgcttcgt gtagcggcgc tggctcctgc tcgacggcat ggccgctgac cagcgcctcc
      961 tgcgcggcgc gcaaccgtgt ctggacctcg cgctgcatct cctcgacttc gctcggagtg
     1021 atgatgccgt cggagatgag gcgatcggcg tagagggcgc ggacggtggg gcgagcggcg
     1081 atgctggcat agagtacggg ctgcgtgaag gccggctcgt cgccttcgtt gtggccccag
     1141 cgacggtagc caacgaggtc gatcagcgca tccttatgga agcgggtgcg gtaggcctcg
     1201 gcgatgccca cgactgtcag gcatgcctcg ggatcgtcag cgttgacatg gaagatggga
     1261 atctcgaagc ccttggcgag gtcgctggcg tagagtgtgg agcgaccatc gtgcggcacg
     1321 gtggtgaagc cgatctggtt gttggcgatg atgtgcagcg tgccgccggt ctgatagccg
     1381 ggcaggcgcg agaggttcag ggtctcggcg acgacgccct caccggggaa ggcggcgtcg
     1441 ccgtggatca ccagcgccag cgcccagtcg gcgtcctggt ggggctcgcc gggggcggca
     1501 cgatcctcct gagcagcacg ggtaaagcct tcgatgaccg gattgacgaa ttcgaggtgg
     1561 ctggggttgt gcgccagggt caactgcacg tgcgcggctt cgccgttgcg catcagcttg
     1621 cgggcgccga ggtggtattt cacgtcgccg gtccagccgt agttgatacc ggtggagcct
     1681 tccgacggga caagctcttt attgggagcg ctgtggaact ccgagaagat cttggcgtag
     1741 ggcttgccga ggatgtgcgc caggacgttg agcctcccgc gatgcgccat gccgatcagc
     1801 acttcgttcg tgccggtctc ggtggcctcg tggatcgcct catcgagcat cggcacgagc
     1861 atgtcgatgc cttcgatgga gaagcgcttc tgtccctgga aggtgctgtg gaggaatcgc
     1921 tcgaagccct cgacttcgct gaggcggcgc agcaggtcgc ggcggccgtc atcggtgagc
     1981 ttcggctgat atgcgccgga ttcgacggcc tgatagagcc aggtgcgctc gtcgaagtct
     2041 tgcacgtggt cgaactcgta gccgatggag cctgagtaga tggcgcgcaa ccgctggatg
     2101 gcgtcgaggc aggtgccagc gcgggaatcg gcgtcgggcc agacgatggt ggcggggagc
     2161 gcagccaggc cggcgtcggt gaggccgtga gtggcggcgt ttagcatcgg atcgcccagc
     2221 ggtggtgagc caaggggatc gagccgcgca tccaagtggc cgtactcgcg gatgctgcg
//
LOCUS       DASVOR010000597        15789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443758,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000597 DASVOR010000000
VERSION     DASVOR010000597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15789
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443758"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2430
                     /locus_tag="VF516_11745"
     CDS             <1..2430
                     /locus_tag="VF516_11745"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX8108396.1"
                     /translation="VLLAAFSEVLATWSKSSRFTLNVTLFNRLPLHPQVNDIVGDFTS
                     VNLLAVDASAGADFESRARAVQHQLWEDLEHSRVSGVHVLRELARAHNSAPRALMPIV
                     FTSTLATSYGADEDGFPIDWLGEIAYSISQTPQVWIDHQVFERRGALMFNWDAVEAIF
                     PPGLLDDMFEAYQALLVRLANKPRSWTDTERHVIPPHDLAVQAAANATDAPVPAGLLH
                     TLFEDQAERRPDAIAVISEGRTLSYRELLQRSTAVARELRARGARPERLVAVVMDKGW
                     EQVVAALAVLQAGAAYVPISPAVPRERLAYLLEHSQVELALTQPGVDARLEWPAGVER
                     LCVTDREPEAGERLAPVQTAENLAYVIYTSGSTGLPKGVMIEHRAAVNTILDMNQRFG
                     VGPGDRILALSSLTFDLSVYDIFGALAAGGTIVLPAPSATRDPACWSELMRRTQVTIW
                     DSVPALLQALVDYADGDAARLPGSLRLVLMSGDWIPVSLPDRIRALVDGVQVYSLGGA
                     TEAAIWSILYPIEAVDPGWKSIPYGKPMVNQRFHVLNELLESCPIGVPGQLFIAGVGL
                     ARGYWRDETKTARSFFVHPRTGERLYRTGDLGRYLPDGNLEFLGREDFQVKVNGFRVE
                     LGEIEAALEQHPDVAQAVAAAVGEVTARRLVGYVVPRTGAALTASQLREFLGAKLPAY
                     MVPGSFVVVPALPLSANGKVDRAALAALDPAPAAVPRAVPAPLAAQPLRIAQLEHLII
                     RVLEIEAIDPEANLLDLGVSSLEMIRIANLLQQELGARPQIDEFYRSPTLHGLAQWYA
                     ETDLAEHDHAC"
     gene            2420..3226
                     /locus_tag="VF516_11750"
     CDS             2420..3226
                     /locus_tag="VF516_11750"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8108397.1"
                     /translation="MLAEPVVNPWIVRPRPNPRARLRLFCFPYAGGGASTYRGWPAHL
                     PADIEVAAVQLPGREERLREPAFTSASELCTQLVAVLAPYLDRPFALFGHSMGGLVAF
                     ELTRRFRTSEGLSPVHLFVSAHCGPRKAYCLPPVTGMPDRELLQLIRRLGGTRDEVLQ
                     DGDVMRLMLPLLRADLTICETYKYVAAEPLDCPISAFGGIFDEVVRRADVLAWAAETR
                     GGFQARMLPGGHFFLDDAKPRLLQAIADDLAARGASSTISISMHGGRLSP"
     gene            3223..5052
                     /locus_tag="VF516_11755"
     CDS             3223..5052
                     /locus_tag="VF516_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX8108398.1"
                     /translation="MTTSIKTLVELLRTRAETHGNRLAFTYLKDGEQVEDAWTYEDLD
                     RRARQIAGALQAVGRDGDRALLVYPPGLAFVAAFFGCLYARRIAVPMSPPNPARLARE
                     LPRFRAILESADPRTLLTCSSIAPIAEALRGSDPALASVQILATDVLAGDEHVWRDPG
                     ADPDTLAFLQYTSGSTALPKGVMVSHGNLLHNMAAIARNFRSTDETPMVSWLPVFHDM
                     GLIGCVLHPILHGSSNVLMAPAAFLQRPLRWLQAISRFQAHTSGAPNFAYDLCVRRIA
                     PDQRATLDLRSWRVAFNGAEPVRARTIAEFTRAFEPCGFRPDAMAPVYGLAEATLLAT
                     GNPRGTPPPVATFHSSDLERARARPASAPWDTVRELVACGRVPSGQRLVIVDPERCTT
                     LPDGEIGEIWLAGPSVAQGYWNRPDETQATFGARLADSGDGPFLRTGDLGFVHDGQLF
                     IAGRRKDLIIVDGRNHYPQDIELTVEQSHPAFRAGCSAAFSIDVDGAERLVVVAEADP
                     SWNIASAGAGSADAKTPLIRAARRAVAMDHDLDLHQLLLLRPASIPKTTSGKIRRQAC
                     RAGFLDGSLPSWEASVAAPSGPPQIVRAPDLAAQTVRDREPRA"
     gene            5166..5870
                     /locus_tag="VF516_11760"
     CDS             5166..5870
                     /locus_tag="VF516_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8108399.1"
                     /translation="MEPVLRAEALAKSYQMGEVEVRALRGVDLEFGAGELVVLLGASG
                     SGKSTLLNILGGLDVPSTGRIVYRGRDLTTARESARTAYRRDHVGFVFQFYNLVPSLT
                     ARENVAIVTDLAEHPLSPEKALEIVGLSHRLDHFPAQMSGGEQQRVAIARAIAKRPQI
                     LLCDEPTGALDVATGKLVLAAIARVNRELGSTTVVITHNAVIAEMADRVLQLADGRVT
                     ADRSNSSKRDPAELAW"
     gene            5870..8236
                     /locus_tag="VF516_11765"
     CDS             5870..8236
                     /locus_tag="VF516_11765"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8108400.1"
                     /translation="MRALDRKLFRDLARMKAQAIAIALVVASGVALFVATMTTYRSLR
                     TSEAVFYRQQHFAQVWSGLGPGRAPLPVARDIAALPGVTAVDARLVTRAILDVPGLDV
                     PASAVLVAIPARPGHTLNDLYLRRGRHVEPGRTDEVMASEAFAEKNHLGPGDSITATV
                     AGHRTALHIVGVALSPEFVMPVEPGSAAPDDQRFGILWIARDQLEALLDMRGSFNDIA
                     LLLAPGTPEHPVIAAIDRILAPYGGAGAYARVNQGSHVMLEEHIAQLRGLTLLVPSIF
                     LLVAAFLVNVVLARLIATQREQIGMLKAFGYSNARVAIHYLELTLAIVVLGVVLGIPI
                     GAWLGRLMAVFYAKFFRFPVLVFELEPVVALGGASVALAAAMAGALGTLRRVVGMPPI
                     VAMTPEVPAFRRTLLDRIGITPLLSSATRMIVRNLTKRPLRSGLASAGMALAVAVVVL
                     GGSSADGFHRMRDVQFEAVQREDLNVALDHPRALGTARDFLHLPGVRQVEPYRVVPAR
                     LLGRGHSEDIALYGLPDGGVLRNVVDTGFRRVPRLGAGMVLTGWTARRLGLVPGDLLA
                     IEIRENHRRVVTAKLEATVEDPLGEAGYMELGALGRLLGEPETYSNASLLIDPTRAPA
                     LYDQLEHAPHAMAIGIRRSTLASFRSMSETSLDFVRTIVIVFSIIIAFGVVYNTARIA
                     LAERGRELATLRVLGFTRGEASGILLGEIAILAAPAIPLGMALGYLLTGAVAASMTGS
                     RMHVPVVVSLSTYAFAVLVFAASALISALVVRRRIDRLDLVEVLKARE"
     gene            8238..9419
                     /locus_tag="VF516_11770"
     CDS             8238..9419
                     /locus_tag="VF516_11770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8108401.1"
                     /translation="MRRRHLLRRLVWTAFVAAATFAVVWALRPRPITVETVAVTRGPL
                     TATVVGEGRIRVKDLFVVSAPVDGQLERISLQAGDLVRAGDVIARIRPIDPRPLDARS
                     RAEGRAAVEVARAAVERAEATEQEAGVAVEHADSELARSQKLAQTGAVARAELEHAGH
                     ESGIRHHAQEAAHAAVREARAQLIRANAAVAPATAPGDAPVSITAPSSGRILRVLRES
                     AGPVAAGTPVIEIGDVTSLEIRADLLSSDATPVRVGAAATVTSWGGPRPLAARVRRIE
                     PAAFTKISALGLEEQRVHVVLDFVDPPPPGLGHDYRVDLAVVTWSGTDVLRVPARSLF
                     RVGDRWAAFVVRDGRAHQALVELGETDGTLTVITRGLATGDVVIAQPTDAITDGTRVR
                     R"
     gene            9470..10183
                     /locus_tag="VF516_11775"
     CDS             9470..10183
                     /locus_tag="VF516_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108402.1"
                     /translation="MGSALETLIKKWREDPGGTYRSWFLWEERLKNFRSIRRGILQVI
                     EEIENGTFGVAYRGSSLETVLDSIAEQRQIFKGADHAFMWKPKLRIPDIYESPDNQRA
                     FGRFLDTCVCCSGGQAIVDAIHALDRKAIKGLGPAAANILYFLHPTHAPPSNTAIVKG
                     YNALCGTRVKLGSWADYLAMREGILRLNDANRALLSNDLGAVAGLLLDVGSGRYQAPP
                     RDDDASARAAWEAELARSA"
     gene            10180..10512
                     /locus_tag="VF516_11780"
     CDS             10180..10512
                     /locus_tag="VF516_11780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108403.1"
                     /translation="MIDVLWLERPGGRVVAAFEVEHTTSIYSGIIRLLDLALGASAHA
                     TRGLFLVAPDDREDEVRAQLTRPAFQRVSDLQVRYLPYGELERNREAMARFGSGLKAI
                     EAVARTLG"
     gene            complement(10531..10755)
                     /locus_tag="VF516_11785"
     CDS             complement(10531..10755)
                     /locus_tag="VF516_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108404.1"
                     /translation="MKTKQPAPHRPASEPVASPFESIDAGQLDAITGGCACGCGQASC
                     SCTSGSCGQGARTTTTTPANPYQFQYRWGR"
     gene            10954..11628
                     /locus_tag="VF516_11790"
     CDS             10954..11628
                     /locus_tag="VF516_11790"
                     /inference="COORDINATES: protein motif:HMM:NF044515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8108405.1"
                     /translation="MCLCQDELFASPSSRILGAMPKLWNETIETHRHDVREAILDATM
                     ALVAEHGPLSVTMSQVAEKTGIGRATLYKYFIDVEAILVAWHDRQVSHHLEQLAELCR
                     QPGDAWSRLQAVLEGFALVRHHHHDPDIAALVHRGERIGQAHRQLTDLIAGLLTEAAA
                     AGRVRDDVTPSELAVYCIHAVTAAAGLPSKAAVLRLVQVTLAGLRPDHRPRAGGDRKR
                     RSRRPA"
     gene            12015..13283
                     /locus_tag="VF516_11795"
     CDS             12015..13283
                     /locus_tag="VF516_11795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108406.1"
                     /translation="MEHEHATAPMAHDEAGTPLGIPETRDASGTAWQPDSTPMFMWHW
                     MTGDWSVGLHTNVFAGPAGASSDRGADKFISINWVMAMARRPVGDGDLLLRAMLSLEP
                     FTVGKSGYPLLLQTGESLDGLPLHDRQHPHDLFMELAARYRQPITDSVGLEVYAAPVG
                     EPAIGPPAYPHRFTAMSNPLAPLGHHWEDSTHISFGVLTAGLFTRQLKLEGSWFNGRE
                     PDEDRYDLDLRMPDSFAARLTVNPTADVSAQVSWARLDSPEQLEPGVSVRRLTASVMW
                     NQQSADRMRDLALVGVLGRNDPSMGPVTTAGLAEAALMLHSEHTLFTRAEVLSKSGHD
                     LALPAALGDQTFTFASFSAGYVYDASWISDVVPGIGFVATVDAIGAGLEPFYGTRVPW
                     GGMVFVRLRAPEMAEMHEHGSMRGHDMHGM"
     gene            complement(13487..14296)
                     /locus_tag="VF516_11800"
     CDS             complement(13487..14296)
                     /locus_tag="VF516_11800"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8108407.1"
                     /translation="MILGSGTPIADPERQGPSVAIVVDGVSYIVDAGAGVVRRAAATK
                     IEALRARNMKLVFLTHLHSDHTLGYPDLLLSGAVLHRGAPLVAYGPHGLRDMTDHLLA
                     AWKQDIELRVNGLERGDAQAYAVDVHEIAPGVVYRDDKVTVRAFLVKHGSWDEAFGYR
                     FDGPDRSIVFSGDTAPADAVVEACNGCDVLIHEVYSRAGFETLPPDDRRYHAAFHTSA
                     PELAALATKARAKTLVLHHQLFFGQTESELIGEVRRGFRGSLVSAHDLQTL"
     gene            complement(14799..14996)
                     /locus_tag="VF516_11805"
     CDS             complement(14799..14996)
                     /locus_tag="VF516_11805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108408.1"
                     /translation="MERLWSQLAPHRQAGEITGVVADAVRPRRELVLENAVLRHQVNV
                     LRRRSTRPKLHLVDRLKLLIA"
     gene            complement(15198..>15789)
                     /locus_tag="VF516_11810"
     CDS             complement(15198..>15789)
                     /locus_tag="VF516_11810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108409.1"
                     /translation="IIGPGDIDAALTIGVGFETFKVPVLPEMETIQDLIVERVKAQLK
                     QRIVETFQARQPARLELEALVKQVYEDVRNEILGLENVRPKIFERPQFTVGFEANRLL
                     GADRWLGRTRVGIGVWKVTLALSAAVGRVCRGGTCDSGVKAFIGPEIVVHALPSKDPR
                     ASVIDAFVRADFQANGRGVETYDQLVLGARYLLDVF"
BASE COUNT         2291 a         5587 c         5377 g         2534 t
ORIGIN      
        1 gtgctgctgg cggcgttctc cgaggtcctg gcgacctgga gcaagagctc ccggttcacg
       61 ctcaacgtca cgctgttcaa tcgcctgccg ctgcatccgc aggtcaatga catcgtcggc
      121 gacttcacgt ccgtgaacct gctcgccgtc gacgcatcgg ccggtgcgga cttcgagtcg
      181 cgcgcgcggg cggtccagca ccagctctgg gaggatctgg agcacagccg cgtgagcggc
      241 gtccacgttc tgcgcgagct cgcgcgcgcc cacaacagcg cgccccgggc gctgatgccg
      301 atcgtgttca cgagcacgct cgcgacgtcg tacggtgccg acgaggatgg cttcccgatc
      361 gactggctcg gcgagatcgc gtacagcatc tcgcagacgc cgcaggtctg gatcgaccac
      421 caggtgttcg agcgccgcgg cgcgctgatg ttcaactggg acgccgtcga ggcgatcttc
      481 ccgcccggtc tgctcgacga catgttcgag gcctaccagg ctctgctcgt ccggctcgcc
      541 aacaagcccc ggagctggac cgacaccgag cgccacgtca ttccgccgca cgatctcgcc
      601 gtgcaggcgg ctgccaacgc caccgacgca ccggtccccg ccggcctcct gcacaccctg
      661 ttcgaggatc aggccgagcg ccggccggac gccatcgccg tgatctccga gggccgcacc
      721 ctcagctacc gcgagctcct ccagcgctcg accgcggtcg cccgcgagct gcgcgcccgt
      781 ggcgcccggc ccgagcggct cgtcgcggtc gtgatggaca agggctggga gcaggtcgtg
      841 gccgcgctcg cggtgctcca ggccggtgcc gcgtacgtcc cgatctcgcc cgccgttccg
      901 cgcgagcgcc tcgcctacct cctcgagcac agccaggtcg agctcgcgct gacccagccg
      961 ggcgtcgatg cccggctcga gtggccggcg ggcgtcgagc ggctgtgtgt caccgaccgc
     1021 gagcccgagg ccggcgaacg gctcgcgccg gtgcagaccg ccgagaacct cgcgtacgtc
     1081 atctacacct ccgggtccac cgggttgccc aagggggtca tgatcgagca ccgcgccgcg
     1141 gtcaacacga tcctcgacat gaaccagcgc ttcggcgtcg ggcccggcga tcgcatcctg
     1201 gcgctgtcgt cgctgacgtt cgatctgtcg gtctacgaca tcttcggcgc gctcgcggcc
     1261 ggcggcacca tcgtcctgcc cgcgccatcg gcgacgcgtg accccgcgtg ctggtccgag
     1321 ctgatgcggc ggacgcaggt cacgatctgg gactcggtcc cggcgctcct gcaggcgctg
     1381 gtcgactacg ccgatggcga cgcggcgcgg ctgcccggct cgctgcgcct cgtgctgatg
     1441 agcggcgact ggatccccgt gagcctcccg gatcgcatcc gcgcgctcgt cgatggcgtc
     1501 caggtgtaca gcctcggcgg agcgaccgag gccgcgatct ggtcgatcct gtacccgatc
     1561 gaagccgtcg atccgggctg gaagagcatc ccgtacggca agcccatggt gaaccagcgc
     1621 ttccacgtcc tgaacgagct gctcgagtcg tgcccgatcg gcgtcccggg acagctgttc
     1681 atcgccggcg tcggtctggc gcgcggctac tggcgcgacg agaccaagac cgccaggagc
     1741 ttcttcgtcc acccgcgcac gggggagcgg ctgtatcgca ccggcgatct cggtcgctac
     1801 ttgccggacg gcaacctcga gttcctgggc cgcgaggact tccaggtcaa ggtgaacggc
     1861 ttccgcgtcg agctcggcga gatcgaggcg gccctcgagc agcaccccga cgtcgcccag
     1921 gcggtcgcgg cggcggtcgg cgaggtcaca gcgcggcggc tggtcggcta tgtcgtcccc
     1981 cggacgggag ccgcactcac cgcgagccag ctgcgcgagt tcctgggcgc gaagctcccg
     2041 gcgtacatgg tgccgggcag cttcgtcgtc gtcccggcgt tgccgctgtc ggccaacggc
     2101 aaggtcgacc gggccgcgct cgcggcgctg gaccccgcgc cggccgccgt gccccgcgct
     2161 gtgcctgcgc cgctggcggc gcagccgctc cggatcgcac agctcgagca tttgatcata
     2221 cgagtcctcg agatcgaggc catcgatccc gaagcaaacc tgctcgacct cggcgtgagc
     2281 tcgctcgaga tgatccggat cgcgaacctg ctgcagcagg agctcggcgc gagaccgcag
     2341 atcgacgagt tctatcgctc tcccaccttg cacggcctcg cgcagtggta cgccgagacc
     2401 gacctcgcgg agcacgacca tgcttgctga gccggttgtc aatccgtgga tcgtccgccc
     2461 gcggcccaac ccacgcgccc gcctgcgcct gttctgcttc ccctacgcgg gcggtggcgc
     2521 ctcgacctac cgcgggtggc cggctcacct cccggcggac atcgaagtgg cggcggtcca
     2581 gctcccgggc cgcgaggagc gcctgcgcga gccggcgttc acgagcgcgt cggagctgtg
     2641 cacgcagctc gtcgcggtgc ttgcgccgta cctcgatcgc ccgtttgcgc tgttcggtca
     2701 cagcatgggt gggctcgtcg cgttcgagct gacccgccgg ttccgcacga gcgaaggcct
     2761 gtccccggtg cacctcttcg tctcggcgca ctgcggaccg cggaaggcgt actgcctgcc
     2821 tccggtgacg gggatgcccg accgcgagct gttgcagctg atccggcgcc tgggcggcac
     2881 gcgggacgag gtgctccagg acggcgacgt catgcggctc atgctgccgc tgctccgcgc
     2941 cgacctgacg atctgcgaga cctacaagta cgtcgccgcc gagccgctgg attgtccgat
     3001 ctcggcgttt ggcgggatct tcgacgaggt cgtccggcgc gccgacgtgc tggcctgggc
     3061 cgccgagacc cgcggcggct tccaggcccg catgcttcct ggtgggcatt tcttcctcga
     3121 tgacgcaaag ccacgcctgc tgcaggcgat cgccgacgat ctcgccgcgc gtggcgcttc
     3181 ctcaaccatc tcgatctcga tgcacggcgg aaggctctcc ccatgactac ctcgatcaag
     3241 acgctcgtgg aattgctgcg gacgcgggct gaaacgcatg gcaatcggct cgcgttcacc
     3301 tatctgaagg acggcgaaca ggtcgaggac gcctggacgt acgaggacct cgaccgtcga
     3361 gcgcggcaga tcgcaggcgc gctgcaggca gtcggccggg acggcgaccg ggcactcctg
     3421 gtctatccac ccgggctggc cttcgtggcc gccttcttcg gctgcctcta tgccaggcgc
     3481 atcgcggtgc cgatgtcgcc gccgaatccc gcgcggctgg cgcgagagct gccacggttc
     3541 cgggccatcc tcgagagcgc ggacccgcga acgctcctga cctgctcgtc gatcgcgccg
     3601 atcgcggagg cgctgcgcgg ctcggatccc gcgctcgcga gcgtccagat cctggcgact
     3661 gacgtcctcg ccggcgatga gcacgtctgg cgcgatcccg gcgccgaccc ggacacgctg
     3721 gcgttcctcc agtacacctc cggttccacg gccctgccca agggcgtcat ggtcagccac
     3781 ggcaacctgc tgcacaacat ggcggcgatc gctcggaact tccgctcgac cgacgagacg
     3841 ccgatggtga gctggctgcc ggtgttccac gacatggggc tgatcggttg cgtgctccat
     3901 ccgatcttgc atggttcgtc gaacgtgctg atggcgccgg cggcgttcct gcaacggccg
     3961 ctgcgctggc tccaggcgat ctcgcggttc caggcacaca ccagcggcgc accgaacttc
     4021 gcctacgatc tgtgcgtccg ccggatcgcg ccggaccagc gcgcgacgct cgacctccgg
     4081 tcgtggcgcg tggcattcaa cggcgccgag ccggtgcgcg cccgcacgat cgcggagttc
     4141 acccgcgcgt tcgagccctg cggattccgg cccgacgcga tggcgccggt ctacggcctc
     4201 gcggaggcga ccctgctggc gaccgggaat cctcgcggga cgccgccgcc ggtcgcgacg
     4261 ttccacagca gcgatctcga gcgcgcccgg gcgaggcctg cgagcgctcc gtgggacact
     4321 gtccgcgagc tggtggcctg tgggcgggtg ccctcgggac agcgactcgt catcgtcgac
     4381 ccggaacgct gcacgaccct gcctgatggc gagatcggcg agatctggct ggcaggaccg
     4441 agcgtcgcgc agggctactg gaaccgcccc gacgagaccc aggccacgtt tggcgcgcgc
     4501 ctcgccgact ccggagacgg tccgttcctc cggaccgggg atctcgggtt cgtccacgac
     4561 ggacagctgt tcatcgccgg ccgccgcaag gacctgatca tcgtcgatgg acgcaaccac
     4621 tatccccagg acatcgagct cacggtggag cagagccacc cggccttccg cgctggctgc
     4681 tccgcggcgt tctcgatcga cgtcgatggc gccgagcggc tcgtcgtggt cgccgaggcc
     4741 gatcccagct ggaacatcgc cagcgcgggt gctggtagcg ccgacgcgaa gactccgctg
     4801 atccgtgcag ctcgccgcgc ggtcgcgatg gaccacgatc tcgatctgca ccagctattg
     4861 ctcctgcggc cggcgagcat cccgaagacg acgagcggaa agatccgccg gcaggcctgt
     4921 cgagctgggt tcctggacgg atcgcttccg tcgtgggagg cgtccgtggc tgcgccgtcc
     4981 ggccctccgc agatcgtgcg ggccccggat ctcgcggcgc aaaccgttcg cgaccgcgaa
     5041 ccgcgtgcgt gagcgcccgg gagctggaga tggcgcgaaa gacgcgcaag ttcgtcgcaa
     5101 cgcgccggtt tttccgctcc gcgaacgatc ccgaccctgg acttcacctt gcagcattcc
     5161 ccgatgtgga acctgtactg cgtgccgagg cgctggcaaa gagctaccag atgggcgaag
     5221 tcgaggtccg cgcattgcgt ggcgtcgatc tcgagttcgg cgcgggggag ctggtcgtgc
     5281 tgctgggggc gtcgggcagc ggcaagtcga ccttgctcaa catcctcggc ggcctcgatg
     5341 taccgagcac cggccggatc gtgtaccgcg ggcgcgacct gacgacggcg cgcgagagcg
     5401 cccgcaccgc gtaccgccgc gatcatgtcg gtttcgtgtt ccagttctac aacctcgtac
     5461 cgagcctgac cgcgcgtgag aacgtcgcga tcgtcaccga cctcgccgag cacccactat
     5521 cgccggagaa ggcgctcgag atcgtcggac tgtcgcaccg gctcgatcac ttcccggcgc
     5581 agatgtcggg cggggagcag caacgcgtcg cgatcgcgcg ggcgatcgcc aagcggcccc
     5641 agatccttct gtgcgacgag ccgaccggcg cgctcgacgt cgcgaccggc aagctcgtgc
     5701 tggccgccat cgcccgcgtc aaccgcgagc ttggcagcac gaccgtggtg atcacgcaca
     5761 acgcggtgat cgccgagatg gccgatcgcg tgctgcaact cgccgacggt cgggtcacgg
     5821 cggaccgcag caactcatcc aagcgcgatc ctgcggagct cgcctggtga tgcgtgcgct
     5881 cgatcgcaag ctgttccgcg acctcgcccg catgaaggcg caggcgatcg cgatcgcgct
     5941 ggtggtcgct tcgggtgtcg cgctgttcgt cgcaacgatg acgacctacc gatcgctgcg
     6001 gacgtccgag gccgtgttct accgccagca acacttcgcc caggtctggt ccggcctcgg
     6061 gcccggacgc gcgccgctgc ccgtggcgcg cgatatcgcg gcgctgccag gtgtcaccgc
     6121 ggtcgatgcc cggctcgtga cgcgcgcgat cctcgacgtc ccgggtctcg acgttccggc
     6181 ctccgccgtg ctggtcgcga tcccggccag gcccgggcac acgctcaacg acctctacct
     6241 gcggcgcggg cgccacgtcg agcccggccg taccgacgag gtgatggcca gcgaggcgtt
     6301 cgccgagaag aaccacctgg gcccggggga ttcgatcacg gcgacggtgg ccggccaccg
     6361 caccgcgctg cacatcgtcg gcgtcgcgct gtctcccgag ttcgtgatgc cggtcgagcc
     6421 cggctcggcg gcacctgacg accagcggtt cgggatcctg tggatcgcgc gcgatcagct
     6481 cgaggcgctg ctcgacatgc gcggctcgtt caacgacatc gcgctcctgc tcgcaccggg
     6541 cacgccggag catccggtga tcgccgcgat cgatcgcatc ctcgcgccct acggaggcgc
     6601 cggcgcgtac gcccgcgtga accaggggtc gcacgtgatg ctcgaggagc acatcgcgca
     6661 gctccgcggc ctcacgctgc tggtcccgtc gatcttcctc ctcgtcgccg cgttcctggt
     6721 caacgtcgtg ctcgcgcggc tgatcgcgac ccagcgcgag cagatcggca tgctcaaggc
     6781 gttcggctac tcgaatgcgc gcgtcgcgat ccactacctc gagctcacgc tggcgatcgt
     6841 cgtgctcggt gtggtgctcg ggattcccat cggtgcgtgg ctcggccgcc tgatggccgt
     6901 gttctacgcg aagttcttcc ggttcccggt gctggtgttc gagctcgagc cggtggtcgc
     6961 ccttggcggg gcgtcggtcg cgttggccgc ggcgatggcc ggcgcgctgg gcacgctgcg
     7021 gcgtgtcgtc gggatgccgc cgatcgtcgc catgactccc gaggtgccgg cattccgccg
     7081 cacgctgctc gaccgtatcg ggatcacgcc gctcctgtcc tcggccacgc ggatgatcgt
     7141 ccgcaacctg accaagcgcc cgttgcgttc gggcctggca tcggcgggca tggcgctcgc
     7201 ggtcgcggtc gtcgtgctcg gtggctcgtc cgccgacggc ttccaccgga tgcgcgacgt
     7261 ccagttcgag gcggtccagc gcgaggatct caacgtcgcg ctcgatcacc cacgcgcgct
     7321 cggcaccgcg cgtgacttcc tccatctgcc gggagtccgc caggtagagc cttaccgggt
     7381 cgtgccggcg cgactgctcg ggcgcggaca cagcgaggac atcgcgctgt acgggctgcc
     7441 agacggcggg gtgttgcgca acgtggtcga caccggcttc cggcgcgtgc cgcggctcgg
     7501 cgcgggtatg gtcctgaccg ggtggacggc gcgccgtctg ggcctcgtac ccggcgatct
     7561 gctcgccatc gagatccgcg agaaccatcg ccgcgtggtc accgcaaagc tcgaggccac
     7621 ggtcgaggat ccgcttggcg aggcgggcta catggagctc ggcgcgctcg ggcggctgct
     7681 gggcgagccc gagacctact cgaacgcgag cctgctgatc gacccgaccc gcgctccggc
     7741 gctctacgat cagctcgagc atgcccccca cgcgatggcg atcgggatcc ggcgcagcac
     7801 gctcgcgagc ttccggtcga tgagcgagac cagcctcgac ttcgtccgca cgatcgtgat
     7861 cgtgttctcg atcatcatcg cgttcggcgt cgtgtacaac accgcgcgga tcgcgctcgc
     7921 cgagcgcggc cgcgagctgg cgaccctgcg cgtgctgggg ttcacccgcg gcgaggcatc
     7981 ggggatcctg ctcggcgaga tcgcgatcct cgcggcgccg gcgatcccgc tcggcatggc
     8041 gctcggctac ctgttgaccg gcgcggtcgc ggcgtcgatg accggctcgc gcatgcacgt
     8101 gcccgtggtc gtgtcgctgt cgacctatgc attcgccgtg ctggtattcg ccgcatcggc
     8161 gctgatctcg gcgctcgtcg tgcgccggcg gatcgatcgg ctcgatctcg tcgaggtctt
     8221 gaaagcgagg gaatgatatg aggcgaagac atctcctccg gcgtctggtc tggacggcgt
     8281 tcgtcgccgc ggcgaccttc gcggtcgtgt gggcgctccg gccgcgcccg atcaccgtgg
     8341 agacggtcgc ggtgacccgc ggccccctga ccgcgaccgt ggttggcgag ggccggatac
     8401 gggtcaagga tctgttcgtg gtctcggcgc cggtcgacgg ccagctcgag cggatctcgc
     8461 tgcaggccgg cgacctggtc agggcgggcg atgtcatcgc gcgcatccgg ccgatcgatc
     8521 ctcgcccgct cgatgcacgc agccgcgccg aggggcgcgc cgccgtcgag gtcgcgcgcg
     8581 ccgccgtcga gcgcgcggaa gccacggagc aggaggccgg cgtcgccgtc gagcacgccg
     8641 acagcgagct cgcgcgctcg cagaagctcg cccagacggg agccgtcgcg cgtgccgagc
     8701 tggagcatgc cggccatgag tccgggattc gacaccacgc gcaggaagcc gcgcacgccg
     8761 cggttcgtga ggcccgcgcc cagctcatcc gcgcgaacgc tgccgtcgca ccggcaactg
     8821 caccgggcga cgccccggta tcgatcaccg cgccgagctc gggccggatc ctccgcgtgc
     8881 tacgcgagag cgctggcccg gtggcggctg gcacccctgt catcgagatc ggcgacgtca
     8941 ccagcctgga gatccgtgct gatctgctgt ccagtgacgc gacgcctgtg cgtgtgggcg
     9001 ctgcggcgac ggtgaccagc tggggtggac ccaggccgct cgccgctcgc gtgcggcgaa
     9061 tcgagcccgc cgccttcacc aagatctcgg cgctcggcct cgaagagcaa cgcgtccacg
     9121 tcgtgctcga cttcgttgac ccaccgccgc cgggactcgg tcacgattac cgcgtcgacc
     9181 tcgcggtcgt gacgtggtcg gggaccgacg tgctccgtgt cccggcgcgc tcgctgtttc
     9241 gtgtcggcga tcgctgggcg gcgttcgtgg tccgtgacgg ccgcgctcac caggccctcg
     9301 tcgagctggg ggagaccgac ggtacgctga ccgtgatcac ccgcggcctg gcgactggcg
     9361 acgtggtcat cgcgcagccg accgatgcca tcaccgacgg tacccgcgtg cgcagatgat
     9421 cgacgcgaga cttcccgaga tcgccgcgaa cgagccacgc cctcgcgcca tgggttctgc
     9481 gttggagacg ctgatcaaga agtggcgcga ggatccgggc ggaacctacc ggtcgtggtt
     9541 cctgtgggag gagcggctga agaacttccg ctcgatccgg cgcggcatcc tgcaggtcat
     9601 cgaggagatc gagaacggga cgttcggtgt cgcgtaccgc ggctcatcgc tggagaccgt
     9661 gctcgactcg atcgccgagc agcgccagat cttcaagggc gccgaccacg cgttcatgtg
     9721 gaagcccaag ctgcgcatcc ctgacatcta cgagtcaccg gacaatcagc gggctttcgg
     9781 ccgcttcctc gacacctgtg tgtgttgctc gggcggccag gccatcgtcg atgcgatcca
     9841 tgccctggat cgcaaggcca tcaagggcct cgggccggcc gccgccaaca tcctctactt
     9901 cctgcaccct acgcacgctc cgccgtcgaa cacggcgatc gtcaagggct acaacgcgct
     9961 gtgtggcacg cgcgtgaagc tcggcagctg ggccgattac ctcgcgatgc gcgaaggcat
    10021 cctccggctc aacgacgcaa accgcgcgct cttgtccaac gacctcggcg cggtcgcggg
    10081 cctgctgctc gacgttggct cgggccgcta ccaggcgccg ccgcgtgacg acgatgcgtc
    10141 cgcccgcgcg gcctgggaag ccgagctcgc gcgatccgct tgatcgacgt gctgtggctg
    10201 gagcggcccg gcggccgcgt cgtggccgcg ttcgaggtcg agcacaccac ctcgatctac
    10261 tccggcatca tccggctgct tgacctcgcg ctcggtgcct cggcgcatgc cacccggggc
    10321 ctgttcctgg tcgcccccga cgatcgtgaa gacgaggtgc gcgcccagct gacgcgccct
    10381 gccttccaac gtgtcagcga tttgcaggtg cgttacttgc cgtacggcga gctggagcgc
    10441 aaccgtgagg cgatggcgcg cttcggctcc ggcctcaagg cgatcgaggc ggtcgcgcgc
    10501 acactcggct gagtggctcg cggctcgtgg tcagcgaccc cagcggtact ggaactggta
    10561 cgggttggcg ggcgtggtcg tggtcgtgcg agcgccctgg ccacagctgc cgctcgtgca
    10621 gctgcaactg gcctggccac agccacacgc gcaaccaccg gtgatggcgt cgagctggcc
    10681 ggcatcaatg ctctcgaacg gcgaggcgac cggctcggaa gcggggcgat gaggggcggg
    10741 ctgcttggtc ttcatggtgg tgatgtctcc tgccgggagc atgagcaacc gctgggcccg
    10801 tggccgggtc catggccggc gaggtgcaag gcggcgaacg cgaggacgaa gacgacggcg
    10861 gcgatcccga gcaccttcac ccagcgcggc gtcggtgcat cgtcggattc ggggcctggt
    10921 ggcaggtcag gcagcggata ctttttcgat acattgtgtc tctgtcaaga cgaactgttc
    10981 gcatccccgt catcacgtat acttggagcg atgccgaagc tgtggaacga gacgatcgag
    11041 acgcaccgtc acgacgtgcg cgaagcgatc ctcgacgcca cgatggcgct ggtcgccgag
    11101 cacgggccgc tgtcggtgac gatgtcgcag gtcgccgaga agaccggcat cggccgagcg
    11161 acgctgtaca agtacttcat cgacgtcgag gccatcctcg tcgcgtggca cgaccgccag
    11221 gtctcgcacc acctggagca gctcgccgag ctatgccgcc agccaggcga cgcatggtcg
    11281 cgcctgcagg cggtcctcga agggttcgca ctcgtgcgcc accaccacca tgaccctgac
    11341 atcgcggcgc tcgttcaccg cggcgagcgc atcggtcagg cgcaccgcca gctcaccgat
    11401 ctgatcgccg ggctgctgac cgaggcggca gcggcaggcc gggttcgcga cgatgtcacg
    11461 ccgtccgagc tcgcggtcta ctgtattcac gccgtcacgg ctgctgcggg cttgccgtcg
    11521 aaagccgcgg tcttgcggct cgtgcaggtc acgctcgctg gtctgcgccc ggatcatcgc
    11581 cctcgcgccg gcggcgatcg caagcgacgg agtcgcagac ccgcataggc agcaagaccg
    11641 ccgcgccgcg tgtccctctg cctgtgcgct ggcgcgcggt cgcgcgcatt cgccgttgct
    11701 ccgcgccatg gctgcctgga gcaaacgcgc ccacaacgtc gagcgtggcg agggctcgct
    11761 ggcaacggct gggatgtcgg gcgcggcgct cctggtacct ggaggcatcc gccttgcagc
    11821 gcgaatcgcg caaaccgcgg cgaggaccca tgtacatctc gatgccgatc caacggccag
    11881 cgccaaatct ccaccgtttg catcgcagtc cggttcgagc ggcgcgcccg ggacctcccg
    11941 gttctcgatg gtggtgctgc gcagtggtgg cgctcggctt gagtgcagcg gccgtccatg
    12001 ctgccgcgca gcccatggag cacgagcacg cgacggcgcc gatggcgcac gacgaggcgg
    12061 gcacaccgct ggggattccc gagacacgcg acgcttcggg aaccgcctgg caaccggatt
    12121 cgacgccgat gttcatgtgg cactggatga ccggcgactg gtcggtcggg ctgcatacca
    12181 acgtgttcgc ggggcccgcg ggcgcctcca gcgatcgcgg cgccgacaag ttcatcagca
    12241 tcaactgggt gatggcgatg gcgcgacgtc ccgttggcga cggtgacctc ttgctccggg
    12301 cgatgctgag cctggaaccc ttcaccgtcg gcaagagcgg ctacccgctg ctcctgcaga
    12361 ccggcgagag cctcgacggc ctgccgcttc acgaccggca acatccccac gacctgttca
    12421 tggagctcgc ggcgcgctat cgccagccga tcacggactc cgtgggcctg gaggtctacg
    12481 ccgcgccggt gggcgagccg gcgatcggtc cgccggcgta cccgcaccgc ttcaccgcga
    12541 tgtccaaccc gctcgcaccg ctcggccatc actgggagga ttcgacgcac atctcgttcg
    12601 gcgtgctcac cgccggcctg ttcacccgcc agctcaagct ggaaggatcc tggttcaatg
    12661 gccgcgagcc cgacgaggac cgctacgatc tcgacctgcg catgccggat tcgttcgcgg
    12721 cgcggctgac cgtcaacccg accgccgatg tcagcgctca ggtctcgtgg gcgcggctcg
    12781 atagcccgga gcaactcgag ccgggtgtca gcgtacggcg tctgacggcc tcggtgatgt
    12841 ggaaccagca gtccgcagat cgcatgcgcg acctggcgct ggtcggcgtc ctcggacgca
    12901 acgatccatc catgggtcca gtgaccacgg cgggcctggc cgaggccgcg ctgatgctgc
    12961 attccgagca taccctcttc acgcgggccg aggtcctgtc caagtccggt cacgacctgg
    13021 cgctgccagc cgcgctgggg gaccagacct tcacgttcgc gagcttctcc gcgggctacg
    13081 tctacgacgc gagctggatc agcgacgtcg ttcccggcat cggcttcgtc gcgacggtcg
    13141 acgcgatcgg cgccggcctg gagccgttct acggcacgcg tgttccgtgg ggcggcatgg
    13201 tgttcgtccg gctgcgcgcg ccggagatgg cggagatgca cgagcacgga tcgatgcgtg
    13261 gccacgacat gcacggcatg tgagccacgg cgatctcttt caccgcagcg acgatccgcg
    13321 gatgctcgct gtcgggatcg aagctcggat ctgccactcc gtgatcgacg acgattggaa
    13381 ttgccggcac atcggctcag gtgctccgcg ggctccgctg cagcccggcc acggcacgga
    13441 tcctgcgtgc tgtagaagcc gagcacggcg gacgggacag catgtgtcag agcgtctgca
    13501 gatcgtgcgc ggagacgagg ctgccgcgga agcctcgtct cacctcgccg atgagctcgc
    13561 tctcggtctg accgaagaac agttgatgat gcagaacgag cgtcttcgcg cgcgcctttg
    13621 ttgcgagcgc cgcgagctcc ggcgccgacg tgtgaaacgc cgcgtggtat ctccgatcgt
    13681 ccggcgggag cgtctcgaag ccggcccgcg aatacacctc gtggatcagg acgtcgcatc
    13741 cattgcacgc ctcgacgacg gcgtcggcgg gcgccgtgtc gcccgagaac acgatgctgc
    13801 gatcgggccc atcgaagcgg taaccgaacg cctcgtccca cgatccgtgc ttcacgagaa
    13861 acgcgcgaac cgtgaccttg tcgtctcggt agacgacgcc cggtgcgatc tcgtggacgt
    13921 cgactgcgta cgcctgcgcg tcgccgcgct cgagtccgtt cacgcgaagc tcgatgtcct
    13981 gcttccaggc agcgagtagg tgatcggtca tgtcgcgaag accgtgcggt ccgtacgcga
    14041 ccaggggagc tccccggtga agaaccgcgc cgctcagcag cagatcggga tatccgagcg
    14101 tgtgatccga atgaaggtgc gtgaggaaga cgagcttcat gttccgcgct cgcagcgcct
    14161 cgatcttcgt cgcggccgcg cgccgcacga ccccggctcc ggcgtccacg atgtaggaca
    14221 cgccatcgac gacgatcgcg acgctcggac cctgacgctc ggggtcggcg atcggggtgc
    14281 cgcttccgag gatcacgatc tgcgttcgcg gccgactcgg cgacggcgat ggtggggcgt
    14341 gcgatgccgc gcagcccgcg acgcacagga tcgccgagat cagccggctc gcctgggtgc
    14401 acccggctcg accgcgagcg ccgcacgatc gcgggagccg ctcgatagca tcctgcgtgc
    14461 ccgggggacg cgtcggcgcg tccatgccct caacgtccat gtgaaacatc tcctcgaggt
    14521 cagcggactg catcctcggc aaggggtaaa ccgcctcggc aagctaacgc agggactcgc
    14581 cgcgcgatag cccatgcaga ggcctcgttg gtgagcgcgt ggcccggctg cgcgcctcgg
    14641 gctacgcgtt ctcgtcgatg ccggtgaatc agacctggct atggcgcgag gcgacgctcg
    14701 aagacccgga tggccagcgg atctgcctgt accacgcggg cgtgaaccgt accgaccccg
    14761 tgctggctac cgatcccatc cgtcaggatc atcgtggatc acgcgatcag cagcttcagc
    14821 cgatcgacga ggtggagctt cggccgcgtg ctgcgacgtc ggagcacgtt gacttggtgg
    14881 cgaagcacgg cgttctcgag caccaactcg cgtcggggcc ggaccgcgtc ggcgacgacg
    14941 ccagtgatct cgcctgcctg tctgtgtggc gccaactggg accacagccg ctccacgcga
    15001 gccagaaccc gggtccggat tcgagagaca cttggcacgc gcatccgcca cttggagcac
    15061 gagcaggggt agccggtcga gccccttgcg atctccaact cccgccatgt tcccacccgg
    15121 atgtgcaagg tagccagcac ggggcccgcg cgagtcgtcc ggcgcgatcc agaacacatc
    15181 gccccggtgg atctgcatca gaacacgtcg agcaagtagc gcgccccgag cacgagctgg
    15241 tcgtaggtct cgacgccgcg cccgttcgcc tggaagtccg cgcgaacgaa cgcgtcgatc
    15301 accgaggcgc gcggatcctt cgacggcagc gcgtgcacca cgatctcggg accgatgaat
    15361 gccttgacgc cgctgtcgca cgtgccgccg cggcagaccc ggccgaccgc cgcggacagt
    15421 gcgagcgtca ccttccacac accgatcccc acgcgcgtgc ggccgagcca gcgatcggcg
    15481 ccgagcaacc ggttcgcctc gaagccgacc gtgaactgcg gacgctcgaa tatcttcggg
    15541 cgcacgttct ccagtccgag gatctcgttg cggacatctt cgtagacttg cttgacgagc
    15601 gcctcgagct cgagccgcgc gggctgccgg gcctggaacg tctcgacgat ccgctgcttc
    15661 agctgcgcct tcacacgctc gacgatcagg tcctgtatcg tctccatctc cggcagcacc
    15721 ggcaccttga aggtctcgaa cccgacgccg atcgtcagcg cggcatcgat gtcgccggga
    15781 ccgatgatc
//
LOCUS       DASVOR010000598         7000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_385339,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000598 DASVOR010000000
VERSION     DASVOR010000598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7000
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385339"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..516)
                     /locus_tag="VF516_11815"
     CDS             complement(139..516)
                     /locus_tag="VF516_11815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108410.1"
                     /translation="MSGRLRSLGVALGAALGLSLALAGAIITACYEIPRPACGFLCGP
                     GGACPDGYSCAADMYCHRNDTPSDLMCTRPDALLPADATSADAAASADATAIDAGEDA
                     APDDAPIDAPDDAAIDAAIDAPP"
     gene            609..1397
                     /locus_tag="VF516_11820"
     CDS             609..1397
                     /locus_tag="VF516_11820"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8108411.1"
                     /translation="MATKVDRERLRSLVEPHWRRLYNFVFRLTLDRERAERYLLDIYE
                     QAVEQADRAPEGDCELWLLGIANRLLEERLPRQPEVDFDMLDETLRGEATRTDVVRSL
                     SDPQRDFLLWELKQGCMTAVVNCLPPGERAAFVVCHILKLPDDAAAKSLGITESAYKV
                     RLSRARKKVGDYLAPRCEHVNPMNPCHCPARVGTALHKGFIGKVPGEVSLRKQAEFPY
                     GRYGTGVGHEDVPMRDVAAIYGNLPEPDPPPDLGDRLVSQLAPP"
     gene            1394..2029
                     /locus_tag="VF516_11825"
     CDS             1394..2029
                     /locus_tag="VF516_11825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108412.1"
                     /translation="MIRPLAPRSRPAVACTLALALGATVACRHPAGDPPLANRNAAAP
                     TSGVRAIDWRNRPYVLTGLGTVTVRDGRAEFALDDEGRVSATGATPASFRVDPPLFAD
                     IDGDGTEDAVIGSVLGTGGTGQFSSIAVYTVRGGQVVPLGEIPGGDRGDGGIRHVALA
                     GSAVIVERNVLAEGDGACCASTWRRERWIWRGDQLVEDAAGRGPLQPVTEP"
     gene            complement(2051..3223)
                     /locus_tag="VF516_11830"
     CDS             complement(2051..3223)
                     /locus_tag="VF516_11830"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEX8108413.1"
                     /translation="MASHDDPTLGFATRAIHAGQVPDATTGAVMTPIYYTSTYAQSAP
                     GVHKGYEYSRTHNLTRFALEANLASLEGGAGALCFASGLAATSTLLQLFDAGSHVIAC
                     DDLYGGTFRLFDKVYRRLGFEFTYVDPLRGADAVAAAIKPTTRLVWVETPTNPMLKLV
                     DIAAVAVACKQRGVLLAVDNTFLTPYFQRPLDLGADIVAHSMTKYLNGHSDVVGGALI
                     VKDPALRDRLAFLQNAAGAVPSPMDSFLVLRGTKTLHVRMERHDSNARALASWLEHHP
                     QVEKVIYPGLPSHPQHDLARRQARGFGGMISFVLAGGLDASRRFLSACQIFTLAESLG
                     GVESLIEHPAIMTHASVPPDRRAQLGIADGFIRLSVGIEDLSDLQADLERAFRAAK"
     gene            complement(3287..3775)
                     /locus_tag="VF516_11835"
     CDS             complement(3287..3775)
                     /locus_tag="VF516_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8108414.1"
                     /translation="MNPAEVTTALLIERTLHASPAYRKVDDSLYVIKQGSAYVMINVV
                     PWGDNRAMVRCTSQLVKGVDVDGPLAKQLLELNCHLRFGAFAVDPAEHTVLFTHTILG
                     GHTLDPEELVATLRDVALIADEYDDKLMKKYGGQRMIDLLEEAAMERIMEKHPDSFEF
                     DD"
     gene            3855..5231
                     /locus_tag="VF516_11840"
     CDS             3855..5231
                     /locus_tag="VF516_11840"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8108415.1"
                     /translation="MRLAYVSFAIVLAAARAAAAEDIIRDIVVEGNTKTTSATVELIA
                     KIDVGDDWTADMLDRIKRDLVSSGLFRDVEAFFEQCGPTGVPKCDAPGVRVHLTVHDK
                     HSWVIAPAIYDEPTYFAAGLGFGENNLFGENQKLLLYGQLASRGGDSFFIGAWVLPSI
                     HGSRFYAQLDTFLKQSQVIEYGPAHGYTDIPTPVRQSVLNYLNAGFKLGVDLFWGLKL
                     DARLRGAHVGYGKISALTSDANQIGPGADVNAPPPPGKEGWDVSNEISLAIDRRADWF
                     GVRTGYRYQASYEYGVPQLGSDFHYQIYNLSLWKGVQVLERHNLTAQVNVNVGHHLPF
                     QQEFTMGGTSMRGYVNNQFRGDLHVAANLEYSLPLFTVFGLSVRGLGFFDSGYTTFLT
                     TSNPERTYLPHSAFGDNSTLAPLKNSVGIGTRLFLQQIVIPLLGVDLGYGLEARDVQV
                     YLAIGLTN"
     gene            5191..6894
                     /locus_tag="VF516_11845"
     CDS             5191..6894
                     /locus_tag="VF516_11845"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8108416.1"
                     /translation="MTSKSISRSVSLISALVAGCGGKPAPSSTPAASGAASGPPDVVM
                     VGGDVWTMDPAHPRAQAVAWRGDQVVAVGDDAAVRALAGPSTRILDLHGRSATPGLVD
                     AHCHLYGLGVDLENVSVRGLDSEAATVAMMADAAKTRPASQWLLGRGWDQNRWPGQQF
                     PTRAALDAAISDRPVVLGRIDGHAIWVNSVALRAAGITRATPDPPGGKIVRDAQGEPT
                     GVLIDNAEALVVSRQPEASPELRAQRLRAAAAVAIAAGLTGVHEMGIEEATADAYKKL
                     AAAHQLPLRVHAYLSVPERLERLATPPEPATGRFEMRGIKIYADGALGSRGARLYEPY
                     TDDPGNRGLWRTDPATLARAVDAAVAGGWQVAIHAIGDAAVGSVIDAYLAANRAHPGD
                     HRLRIEHTQVIAAKDVPRMALAHAIASMQPTHGTSDMPWAEARLGPERVKGAYAWRTM
                     LDHHIPFAAGSDFPVEQVPPILGLYAAVTRQDAQGKPAGGWYPQQRMTLSEAIDGFTH
                     GAAYAEGAEATRGVLAVGRHADLTVFSGALAPDRSLLDLRVDYTIVDGEIVYQREQPG
                     R"
BASE COUNT         1018 a         2464 c         2496 g         1022 t
ORIGIN      
        1 gatcgatcgc ggcgcagcca cgctgttcac cggcgctggc tgttccgcgg gcgcgctcga
       61 tgtggcgcag cgccgcttgc ccgacgctgc acagacgcgg accgagccat gggagatgct
      121 gttcggcgac gacgatccct agggcggggc atcgatcgcc gcatcgatcg ccgcatcgtc
      181 gggcgcgtcg atcggtgcgt cgtcgggcgc agcatcctcg ccggcgtcga tcgccgtggc
      241 gtcggcgctc gccgcagcgt cggcgctggt ggcatctgct ggcagaagtg cgtccgggcg
      301 tgtgcacatc aggtcggacg gcgtgtcatt ccggtggcag tacatatccg ccgcgcagct
      361 gtagccgtcc ggacaggcgc cgcccggtcc gcacaggaac ccgcacgccg gcctcgggat
      421 ctcgtagcac gccgtgatga ttgcgccggc caaagcgagc gagagaccga gcgcggcacc
      481 gagggcgacg cccagggacc gtagtcgtcc cgacacccgc agacgatatc atgaacactt
      541 tttgcgtacc ccctcacacc ctcgcgacac gacgtgagat ggccgtcgtg gtgtacagtc
      601 cggagaagat ggctacgaag gtcgaccgcg agcgactgcg ttccctggtc gagccgcact
      661 ggcgccggct gtacaacttc gtgttccggc tgaccctgga tcgcgagcgc gccgagcgct
      721 acctgttgga catctacgag caggccgtcg agcaggccga tcgcgccccg gagggcgact
      781 gcgagctgtg gctgctcggg atcgcgaacc ggctgctcga ggagcggctg ccccgccagc
      841 ccgaggtcga cttcgacatg ctcgacgaga cgctgcgcgg cgaggcgacg cgcaccgacg
      901 tcgtgcgctc gctcagcgat ccgcagcgcg acttcctgct gtgggagctc aagcagggct
      961 gcatgacggc ggtggtcaac tgcctgccac ccggtgagcg cgccgcgttc gtggtctgcc
     1021 acatcctcaa gctgcccgac gacgccgcgg cgaagagcct cgggatcacc gagagcgcct
     1081 acaaggtccg gctgtcgcgg gcgcgcaaga aggtcggcga ttacctcgcg ccgcgctgcg
     1141 agcacgtcaa cccgatgaac ccgtgccact gcccggcgcg ggtcggcacg gcgctgcaca
     1201 agggcttcat cggcaaggtc ccgggcgagg tctcgctgcg caagcaggcc gagttcccgt
     1261 acggccgcta cggcaccggg gtcggccacg aggacgtgcc gatgcgcgac gtcgcggcga
     1321 tctacggcaa cctgcccgag cccgatccgc cgccggacct cggcgaccgg ctggtctcgc
     1381 agctcgcgcc gccatgatcc gcccgctcgc gcctcgctcg cgccccgcgg tggcatgcac
     1441 cctggcgctc gcgctcggcg caaccgtggc atgccgccat cccgcgggtg atccgccgct
     1501 cgccaatcgc aacgccgcgg cacccaccag cggtgtccgc gcgatcgact ggcgcaaccg
     1561 gccctacgtg ctcaccgggc tcggcacggt caccgtgcga gacggccgcg ccgagttcgc
     1621 gctcgacgac gagggcaggg tgtcggccac cggcgcgacc ccggcgtcgt tccgggtcga
     1681 cccgccgctg ttcgccgaca tcgacggcga tggcaccgag gacgcggtga tcggaagcgt
     1741 gctgggcacc ggcggcaccg gccagttctc gtcgatcgcg gtctacaccg tgcgcggcgg
     1801 ccaggtggtc ccgctcggcg agatcccggg cggcgaccgc ggcgacggcg ggatccgcca
     1861 cgtcgcactc gcaggtagcg cggtgatcgt cgagcgcaac gtgctcgccg agggcgacgg
     1921 cgcgtgctgc gcgtcgacct ggcggcgcga gcgctggatc tggcgcggcg accagctggt
     1981 cgaggacgcc gcggggcgcg gcccgctgca gccggtgaca gagccgtgaa gaacatcgac
     2041 acgcccgctg ctacttcgcg gcgcggaacg cgcgctcgag gtcggcctgg agatcgctca
     2101 ggtcctcgat gccgaccgac agccggatga agccgtcggc gatgccgagc tgggcgcggc
     2161 gatccggcgg cacgctggcg tgggtcatga tcgccgggtg ctcgatcagc gactcgacgc
     2221 cgccgagcga ctcggcgagc gtgaagatct ggcacgccga caggaaccgg cgcgaggcgt
     2281 cgagcccgcc ggcgagcacg aagctgatca tgccgccgaa cccgcgggcc tggcggcgcg
     2341 ccagatcgtg ctgcgggtgc gacggcagcc cggggtagat caccttctcg acctgcgggt
     2401 ggtgctcgag ccagctcgcc agcgcgcgcg cgttgctgtc gtggcgctcc atccggacgt
     2461 gcagggtctt ggtgccgcgc aggaccagga agctgtccat cggcgacggc acggcgccgg
     2521 cggcgttctg caggaacgcg agccggtcgc gcagcgcggg gtccttgacg atcagcgcgc
     2581 cgccgaccac gtcggagtgg ccgttgaggt acttggtcat cgagtgcgcg acgatgtcgg
     2641 cgccgagatc gagcgggcgc tggaagtacg gggtcaggaa cgtgttgtcg accgcgagca
     2701 gcacgccgcg ctgcttgcac gccacggcga ccgccgcgat gtcgaccagc ttgagcatcg
     2761 ggttggtcgg ggtctcgacc cagaccagcc gcgtggtcgg cttgatcgcg gcggccacgg
     2821 cgtcggcgcc gcgcagcggg tcgacgtagg tgaactcgaa gccgagccgg cggtagacct
     2881 tgtcgaacag ccggaacgtg ccgccgtaca ggtcgtcgca cgcgatgacg tggctgccgg
     2941 cgtcgaacag ctggagcagg gtcgacgtgg cggcgagccc cgatgcgaag cacagcgcgc
     3001 cggctccccc ctcgagcgac gcgaggttgg cctcgagcgc gaaccgggtc aggttgtggg
     3061 tgcgcgagta ctcgtagccc ttgtggacgc cgggcgcgga ctgcgcgtac gtggaggtgt
     3121 aatagatcgg cgtcatcacc gcgccggtcg tcgcgtcggg cacctgcccg gcgtggatcg
     3181 cgcgcgtggc gaagcccagg gtcggatcgt cgtgcgaagc catggtgtcg tcgtcctcgt
     3241 gctcgtcgtt cgggtcgggg cgggtcggtc gtggctcgat cgtcgctcag tcgtcgaact
     3301 cgaagctgtc ggggtgcttc tccatgatcc gctccatcgc cgcctcctcc agcagatcga
     3361 tcatgcgctg cccgccgtac ttcttcatca gcttgtcatc gtactcgtcg gcgatcagcg
     3421 cgacgtcgcg cagggtcgcg accagctcct cgggatcgag ggtgtggccg ccgaggatcg
     3481 tgtgggtgaa cagcaccgtg tgctcggcgg gatcgaccgc gaacgccccg aaccggaggt
     3541 ggcagttgag ctcgagcagc tgcttggcga gcgggccgtc gacgtcgacg cccttgacca
     3601 gctgcgacgt gcaccggacc atcgcgcggt tgtcgcccca cggcacgacg ttgatcatca
     3661 cgtacgccga gccctgcttg atgacgtaca gcgagtcgtc gaccttgcgg tacgcagggg
     3721 aggcatggag cgtccgctcg atcaggagcg cggtggtgac ctcggcaggg ttcatgcacc
     3781 gttgttagca cgggtgccaa gtcgtgtgat cggcgacgcg cgcggcgcga tcccctcgtg
     3841 ccatactgcg cgccgtgcgc ctcgcttacg tgtcgttcgc gatcgtgctc gccgccgctc
     3901 gcgccgccgc cgccgaagac atcatccgcg acatcgtcgt ggagggcaac accaagacca
     3961 cctcggccac cgtcgagctg atcgccaaga tcgacgtcgg cgacgactgg accgccgaca
     4021 tgctcgaccg gatcaagcgc gacctggtgt cgagcgggct gttccgggac gtcgaggcgt
     4081 tcttcgagca gtgcgggccg accggcgtcc ccaaatgcga cgcgcccggc gtgcgcgtcc
     4141 atctcaccgt ccacgacaag cactcctggg tgatcgcgcc ggcgatctac gacgagccga
     4201 cctacttcgc agccgggctg gggttcgggg agaacaacct gttcggcgag aaccagaagc
     4261 tcctgctcta cggccagctc gcgtcccgcg gcggggactc gttcttcatc ggcgcctggg
     4321 tgctgccgtc gatccacggc tcgcggttct acgcgcagct cgacaccttc ctcaagcagt
     4381 cgcaggtcat cgagtacggg ccggcgcacg gctacaccga catccccacg ccggtgcggc
     4441 aatcggtcct gaactacctc aacgcgggct tcaagctcgg cgtcgacctg ttctggggcc
     4501 tcaagctcga cgcccggctg cgcggggcac acgtcggtta cggcaagatc tccgcgctca
     4561 cgagcgacgc caaccagatc ggcccaggcg ccgacgtcaa cgcgccgccg cccccgggca
     4621 aggaaggctg ggacgtgtcg aacgagatca gcctggcgat cgaccgccgc gccgactggt
     4681 tcggcgtgcg gaccggctac cgctaccagg cgtcgtacga gtacggggtc ccccagctcg
     4741 gctcggactt tcactaccag atctacaacc tgtcgctgtg gaagggcgtg caggtgctcg
     4801 agcgccacaa cctgaccgcc caggtcaacg tcaacgtcgg ccaccacctg ccgttccagc
     4861 aggagttcac gatgggcggg acgtcgatgc gcggctacgt caacaaccag ttccgcggcg
     4921 acctgcacgt tgcggcgaac ctcgagtact cgctgccgct gttcacggtg ttcgggctgt
     4981 cggtccgcgg gctcgggttc ttcgactcgg ggtacaccac gttcctgacc acgagcaacc
     5041 ccgagcgcac ctacctgccg cactcggcgt tcggcgacaa ctcgacgctg gcgccgctca
     5101 agaactcggt cgggatcggc acgcggctgt tcctgcagca gatcgtgatc cccctgctgg
     5161 gcgtggattt ggggtacggt ctcgaggccc gtgacgtcca agtctatctc gcgatcggtc
     5221 tcactaatta gcgcgctggt cgccggctgc ggcggcaagc ccgcgccctc ctccaccccg
     5281 gctgcatccg gcgccgcgtc gggcccgccc gacgtggtga tggtcggcgg cgacgtgtgg
     5341 acgatggacc ccgcgcaccc cagggcgcag gcggtggcgt ggcgcggcga tcaggtcgtc
     5401 gcggtcggcg acgacgccgc ggtgcgcgcg ctcgccgggc catcgacccg catcctcgat
     5461 ctgcacggcc gctcggcgac gcccgggctg gtcgacgccc actgccacct ctacggcctg
     5521 ggcgtcgatc tcgagaacgt gtcggtgcgc gggctggact cggaggccgc aaccgtcgcg
     5581 atgatggccg atgccgcgaa gacccgaccg gcgagccagt ggctgctcgg ccgcggctgg
     5641 gatcagaacc gctggccggg gcagcagttc ccgacccgcg ccgcactcga cgccgcgatc
     5701 tcggatcgcc cggtcgtgct ggggcggatc gacggccacg cgatctgggt caactcggtc
     5761 gcgctgcgcg cggccgggat caccagggcg acgccggatc cgccgggcgg caagatcgtc
     5821 cgcgacgcgc agggcgagcc gaccggcgtg ctgatcgaca acgccgaggc cctggtggtc
     5881 tccaggcagc cggaggccag cccggagctg cgcgcgcagc gcctgcgggc ggcggccgcc
     5941 gtggcgatcg ccgccgggct gaccggtgtg cacgagatgg ggatcgaaga ggcgaccgcg
     6001 gatgcctaca agaagctcgc ggccgcgcac cagctgccgc tgcgcgtcca cgcgtacctg
     6061 agcgtccccg agcggctcga gcggctggcc acgccgcccg agccggcgac cgggcggttc
     6121 gagatgcgcg gcatcaagat ctacgccgac ggcgcgctcg gctcgcgcgg cgcgcggctg
     6181 tacgagccgt acaccgacga tcccggcaac cgcgggctgt ggcgcaccga ccccgccacg
     6241 ctggcccgcg cggtcgacgc cgcggtcgcc ggcggctggc aggtcgcgat ccatgcgatc
     6301 ggcgacgccg cggtgggcag cgtgatcgac gcgtacctcg ccgcgaaccg ggcgcacccc
     6361 ggtgaccacc ggctgcggat cgagcacacc caggtgatcg cggccaagga tgttccgcgg
     6421 atggccctgg cccacgcgat cgcctcgatg cagccgaccc acgggaccag cgatatgccg
     6481 tgggccgagg cgcggctggg ccccgagcgc gtcaagggcg cgtacgcgtg gcgcacgatg
     6541 ctcgatcatc acatcccgtt cgcggcgggc tccgattttc ccgtcgagca agttcctccg
     6601 atcctcgggc tctacgccgc ggtcacgcgg caggatgcgc agggcaagcc ggccggaggt
     6661 tggtatcctc agcagcgaat gacgctgtcc gaagcgatcg acgggttcac gcacggggcc
     6721 gcctacgccg agggcgcgga ggcgacgcgc ggcgtgctcg ccgtgggccg tcacgccgat
     6781 ctcaccgtgt tcagcggtgc gctggcgccc gaccgcagcc tgctcgacct ccgcgtggac
     6841 tatacgatcg tcgacggcga gatcgtgtac cagcgcgagc aaccaggccg gtagcatgcg
     6901 gttctgcccg ttctgcagcg cggagaacgc tgacgatctc gccgtgtgtc agtcctgcgg
     6961 ccggcggctc ccaccgctgc cgccgcgccg cgcgcgcaat
//
LOCUS       DASVOR010000599         7346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_268444,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000599 DASVOR010000000
VERSION     DASVOR010000599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268444"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            412..2193
                     /locus_tag="VF516_11850"
     CDS             412..2193
                     /locus_tag="VF516_11850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8108417.1"
                     /translation="MSKLGEGGMGVVYLAKHVTLGKKAAIKVLRPALSSNQDVVSRFF
                     NEARAVTAVRNPGIVDVYDFGFLEDRTAYIIMEYLEGESLAARLRRSRPAIPWTLTVV
                     RAIARALQAAHEHGIVHRDLKPDNVFLVPDADLPGGERVKLLDFGIAKLAHGVGDVSH
                     TTTGTVMGTPTYMAPEQCRGAGAVDHRADLYSLGCVAYEMLCGQPPFVADGPGDVIAR
                     HLYFEPQPPRSRRAEIPPELEEIVVRLLKKEPRARYATAAELVRAIDDLSGASSSGVA
                     PSKPLATELVATLPVVRDTTLNGAASSSLSLGKDERSRRLGIVTAALTMAVAAFALVW
                     FLAHGRRGDGSGAGAGVALAEPKGSPDSSAASPPVPAPAAPRPATAAEGASPPPAPAA
                     IERAQGSAAGSSGPVGGGTVTPQDPGPKSPVAPVDQHEPESAMAPETSPDPVPAEPPP
                     GPSAASAKPPSDAGGQSGGGRHDARRSETAKPSSGRPSAPRSSVEGPRHAPARPLESP
                     GEPTTPDRGTDPSSGNGPTPVAPVAPGPGAPPDAECSRAAFAGALDARAPSKKAVNDA
                     RSRLARCKPSMDPDLYADIYHRLIERY"
     gene            2326..3552
                     /locus_tag="VF516_11855"
     CDS             2326..3552
                     /locus_tag="VF516_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108418.1"
                     /translation="MFDASGLRLNVSGLSCHVTVVEHWCPVDDPHCRRDPTVTKDEPA
                     ECPHTVAPGKFSLHTPWRQVYPGVAVSGVLGVKIDWKTASVDTVSGVALHTGWSVHSD
                     DGGVDVDATLSLTDAEVKALLAAVRDATGNDYSDAPAGERASIGVVVSSAPIAGDENH
                     VVVSVTNHGPTPAYRVVAQLKSRTPSLQGITLPFGRIDPGTTKQRATDIPTPHDAEAL
                     IDVEAASANATRVQAPQGIRLKPKRVSAAPPPPPPSPPQLSCSAPASQVAAGKRLRVP
                     CEASNPGDQSVKGVTYKVAIASNAPTSAEGPADLAPHAAPSKFDVDVALPSSLAPGVV
                     QVTVMASAPDGPPVEQALSIDVVAPHKLCKPGELTVEDYRRKHKLLDEDRRAGNLTKE
                     EFNDVLAELWSCVRSP"
     gene            complement(3631..4071)
                     /locus_tag="VF516_11860"
     CDS             complement(3631..4071)
                     /locus_tag="VF516_11860"
                     /inference="COORDINATES: protein motif:HMM:NF013996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="desulfoferrodoxin family protein"
                     /protein_id="HEX8108419.1"
                     /translation="MDKRSFLRLTLAGSGLGLFVPKAVFAGMMESPLKSKLAGGMFFT
                     EEALGRWNKGTATHHVPQLQKQTTGGKTQLHVASAHPMDGLDHFIVKHQLLDQDLKFL
                     QEHLFKPTVDKKPEHIFDLGNHHGVVYALTVCNVHDMWVNMIEV"
     gene            4346..5188
                     /locus_tag="VF516_11865"
     CDS             4346..5188
                     /locus_tag="VF516_11865"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8108420.1"
                     /translation="MTIEDARRWYAEEIRAVAHLTSDAVVEALARVPREAFLGAGPWQ
                     IPRSPELAPHYRVTPDADPRHLYHNVLVAIDPARQLHNGLPSALALWIEAADVRPGDR
                     VLHIGAGLGYYTAILAELAGPTGRVVGYEVDAALAARAEAALRPWPTVRIEAGDAAAP
                     AGPFDAIFVNAGCTHPRPDWLAALAPGGRLVIPLTVSLPGLMHSMGAMVRAERRDPRW
                     PAAVVSPCGIYHCTNARDPDHEAELRKVLGSIAKTRPTAAVVEPHERGAACLVHLPGF
                     CLQA"
     gene            5353..6774
                     /locus_tag="VF516_11870"
     CDS             5353..6774
                     /locus_tag="VF516_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108421.1"
                     /translation="MSCLLIASCGGSSSGPSLLPDVTDGGGPVMAHVQLVPIFYANDT
                     DADALTRFSQWIVSSQWLKTVGADYGVDTGSVLGVVHQPGPAPATIDDSDIVALLYAG
                     LANGSLPKPAGGLASALYMIWFPASTTVTAGLDKSCDVFGGYHNSVRQGGVELSYAVI
                     AACSGFVDGLSDLEDREVVASHELIEAATDPLPDNHPSFQLDDPTSSWFGFGTEVGDL
                     CQRGDTTEIVRESGFVAQRSWSTSAAAAEHDPCVPGATADYFNLAVQTPGVLRIAPGG
                     HQAVTLRAWASGAARGTTWQLQTGAAMDSAQTLTLSASMIKDGATVSLDVALPQTAQI
                     GDQPQFVVFSATSDTNYSILPLYAVAGEPCSQFTTCEACTAEVGCGFCASSGKCEAQG
                     GVTSSAQSSCSGGSFATWPGSCKGFCAGHSGSCIDCSSQPGCGWCASGQQHCVEASHE
                     TGQPESGSCPYADWSFTPDYCSQ"
     gene            6897..>7346
                     /locus_tag="VF516_11875"
     CDS             6897..>7346
                     /locus_tag="VF516_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108422.1"
                     /translation="MRLVVVVAVVAVIGLARTAAFADPPGMTPPVPPAPQDPVVAPYY
                     GLTLAADGVALVTAMAALGGGDSESRTRLFQIAAGTYVLGGPLLHVVHGRPGRAAISL
                     AMRVGLPVVAAGLLSSGHDGWDALVPFLLGAVGGTLTASIVDAAVLAR"
BASE COUNT         1048 a         2577 c         2605 g         1116 t
ORIGIN      
        1 catggcgacg cccacgcgat cgtcgccggg cgctcgcgcc gtgcgccatg cgacctcgtg
       61 caagcgcgcg ctcgcggcgc gtgctgcgac gtccgccggg agaactgccg accgtctccc
      121 acatcgtcag ccacgcggtc cgggagatgc ccgcgatgtc cgatcacgtc atggtcgcgg
      181 ccggtgaggt ttgaccaggg ctgtaatcgt gtagatcgag tgtgctgttc gcgccgtacg
      241 acacggtggt ccattggttc ctgatacagc gtcgtactgc atcacttcgc tgcgaaaaaa
      301 caatcgatcg cttgtccggt aatgacgcga tctgcatgta tcgtcatcac cgcttgttag
      361 ctgcgtcggg aggattgcat gctgggccga agcttgggga actacggtgt cgtgtcgaag
      421 ctcggagagg gtgggatggg cgtggtctac ctggccaagc acgtgacgct gggcaagaag
      481 gcagcgatca aggtcctgcg ccccgcgctg tcgtcgaacc aggacgtcgt ctcacgattc
      541 ttcaacgagg cccgcgccgt gaccgccgtg agaaaccccg gcatcgtcga tgtctatgac
      601 ttcggctttc tggaggaccg cactgcgtac atcatcatgg aatacctcga aggcgagagc
      661 ctcgccgcgc gcttgcggcg aagccgcccg gcgattccct ggacgctcac cgtggtccgt
      721 gcgatcgcgc gcgcgctgca ggcggcgcat gagcatggca tcgtccatcg cgacctgaag
      781 ccggacaacg tgttcctcgt gccggacgcg gacctgcccg gcggtgagcg ggtgaagctc
      841 ctcgacttcg ggatcgcgaa gctggcgcac ggcgtcggcg acgtcagtca caccaccacg
      901 gggaccgtca tggggacgcc gacgtacatg gcgcccgagc agtgtcgcgg ggccggcgcg
      961 gtggaccatc gcgcggatct gtactcactc ggatgcgtcg cgtacgagat gctgtgcggt
     1021 cagccgccgt tcgtcgcgga cgggcccggt gacgtgatcg ctcgccacct gtacttcgag
     1081 ccgcagccac cgcgatcacg gcgcgccgag attcctcccg agctggaaga gatcgtcgtc
     1141 cggttgttga agaaggagcc gcgcgcccgc tacgccacgg cggccgagct cgttcgcgcg
     1201 atcgacgatc tgtccggtgc gtcatcgtcg ggcgtggctc ccagcaagcc gctggccacc
     1261 gagctcgtcg cgacgctgcc tgtggtgagg gatacgacgc tgaacggcgc cgcatcgtcc
     1321 agtctgtcgc tcgggaagga cgagcgcagc cgtcggctcg gcatcgtcac cgcggcgctg
     1381 accatggcgg tcgcggcgtt cgcgctcgtg tggttcctgg ctcacggcag gcgcggggac
     1441 ggatccggcg ccggcgccgg ggttgcgctc gccgagccga aggggtctcc ggacagctcg
     1501 gcggcgtcgc cgcccgtgcc tgcaccggcg gcacctcgtc cggcgaccgc tgcggagggt
     1561 gcatcgcccc caccggctcc cgcggcgatc gagcgagcgc agggatccgc cgccggatcg
     1621 tccggcccgg tcggtggcgg cactgtcact ccacaggatc ccggcccgaa gagtccggta
     1681 gcgccggtcg accagcacga gcccgagagc gcgatggctc ccgagacgtc accggatccg
     1741 gtgcccgcgg aaccgccgcc cggcccgtct gcagcctccg cgaagccacc gagcgacgcc
     1801 ggtggccagt ccggcggtgg caggcacgac gcacgcagat ccgagacggc aaagccgtcg
     1861 tctggacggc ccagcgcgcc gagatcatcg gtcgagggcc ctcggcacgc accggcacgt
     1921 ccgctcgaga gtcctgggga accgacgacc ccggaccggg gcaccgaccc gagctccggc
     1981 aacggcccga cccccgtcgc ccccgtcgcg ccgggacctg gagcgccgcc cgacgcggag
     2041 tgttcgcggg ccgcattcgc gggggccctc gacgccaggg ccccgagcaa gaaggccgtc
     2101 aacgacgcga ggtcccggct ggcgcgatgc aagccctcga tggatccaga tctgtacgcc
     2161 gacatctacc accgcctcat cgagcggtac tagcgaggga tctgcatgca acgaacatgt
     2221 ctctcgcttg cgtttgtcag ccttgccgcg ttgtgcggat gcgctccgca gccctatctg
     2281 cgacttcggg gcgcggtcca gtcgacggtc ggggtctccc tgccggtgtt cgacgcatcg
     2341 gggctgcgat tgaacgtctc ggggctcagt tgccacgtca ccgtggtcga gcactggtgt
     2401 ccggtcgacg atccacactg ccgtcgggat ccgaccgtca ccaaggacga gccggcggag
     2461 tgcccgcaca ccgtcgcgcc cggcaagttc tcgctgcaca cgccctggag acaggtgtac
     2521 ccgggcgtcg ccgtttccgg ggtgctcggc gtcaagatcg actggaagac ggcctcggtc
     2581 gacaccgtga gtggcgtcgc gctccatacc ggctggtcgg tgcactccga cgacggtggg
     2641 gtggacgtcg acgcgacgct gtcgctgacc gacgcggagg tcaaggccct gctcgccgcg
     2701 gttcgggacg cgaccggcaa cgactacagc gatgcgcctg cgggcgagcg cgcctcgatc
     2761 ggcgtggtgg tgtcgagtgc gccgatcgcc ggcgacgaga accacgtcgt cgtgtcggtg
     2821 accaatcacg ggcccacgcc tgcgtaccgg gtggtcgctc agctcaagag ccgcacgccg
     2881 tcactgcagg ggatcacgct gccgtttgga cgcatcgacc ccggaacgac caagcagcgc
     2941 gccacggaca ttccgacccc gcacgatgcc gaggcactga tcgacgtgga ggcggcgtcc
     3001 gcgaacgcga cacgcgtcca ggcgccgcag gggatccggt tgaagcccaa gcgggtgagc
     3061 gccgccccgc cgccaccgcc gccgtcgccg ccccagctgt cctgcagcgc tccggcgagc
     3121 caggtggccg cgggcaagcg gctccgcgtc ccctgtgaag cgagcaaccc cggcgaccag
     3181 tcggtgaagg gtgtgaccta caaggtggcg atcgccagca acgcgcccac gtcggcggag
     3241 ggacccgcgg acctcgcacc acatgcggcg ccgagcaagt tcgatgtcga cgtcgcgttg
     3301 ccgagcagtc tcgcaccggg agttgtgcag gtcaccgtca tggcaagtgc accggatgga
     3361 ccgccggtag agcaggccct ctcgatcgac gtcgtggcgc ctcacaagct ctgcaagcca
     3421 ggtgagctca ccgtcgagga ctaccgcagg aagcacaaac tcctcgacga ggaccggcgc
     3481 gcgggcaacc tcaccaagga ggagttcaac gacgtcctgg cggagctctg gagctgcgtc
     3541 cggagcccct gagcgcggcc tcggattcgg ctgcgctccg gcgcggatcg ccttcgagct
     3601 gcgctggagc agcgccccgg cagcgggcga tcacacctcg atcatgttga cccacatgtc
     3661 gtggacattg cacaccgtga gcgcgtacac gacgccgtga tggttgccca ggtcgaagat
     3721 gtgctccggc ttcttgtcca ccgtgggctt gaagaggtgc tcctgcagga acttgaggtc
     3781 ttgatccagc agctggtgct tcacgatgaa gtggtcgagg ccatccatcg ggtgagccga
     3841 ggcgacgtga agctgcgtct tgccgcccgt ggtctgcttc tggagctggg gaacgtggtg
     3901 ggtggcagtg cccttgttcc agcgaccgag cgcttcctcg gtgaagaaca tgccgcccgc
     3961 aagcttggac ttgagcggcg attccatcat gcccgcgaag accgccttgg ggacgaagag
     4021 gcccaggccg ctgccggcaa gagtcagtcg aagaaagcta cgcttgtcca tgtttcctcc
     4081 ctgtcgggtc gagtgggggc ggtggcgcgg accgtaacat gggtcgcacc gagcgggccc
     4141 gccagaccgt gacaagcgcc gccattcatg ccatcgttcc gtcacatagc gcggcgtggc
     4201 tccgcgtgat cctctgacga ccggcccatg cccggtcgcg ctgcgaggac gaaggggggc
     4261 agtcctctca ccaccgtaac ttctcctccg ccgcttcgcg gaccgggtgc atgggagccg
     4321 ccgcggggcg tgactggagt cgaggatgac gatcgaagac gcccggcgat ggtacgcgga
     4381 ggagatccgc gccgtcgcac acctgacctc cgatgccgtg gtcgaggcgc tcgcgcgcgt
     4441 gccgcgcgag gcgttcctcg gtgccggccc gtggcagatc ccgcggtcac ccgagctggc
     4501 gccgcattac cgcgtgacgc ccgacgccga tccgcgccac ctctatcaca acgtgctggt
     4561 cgcgatcgat ccggcgcgcc agctccacaa cggcctgccg tcggcgctcg ccctgtggat
     4621 cgaggcggcc gacgtccgcc ccggcgaccg cgtgctgcac atcggcgccg ggctcggcta
     4681 ctacacggcg atcctcgccg agctcgccgg gccgaccggc cgtgtcgtcg gctacgaggt
     4741 cgatgcggcg ctggcggcgc gcgccgaggc cgcgctgcgg ccgtggccga ccgtgcggat
     4801 cgaggccggt gacgccgccg cgccggccgg gccgttcgac gcgatcttcg tcaacgcggg
     4861 ctgcacgcat ccgcgtccgg actggctcgc cgcgctggcg ccgggcggcc ggctggtgat
     4921 cccgctgacc gtcagcttgc cggggctgat gcacagcatg ggcgcgatgg tgcgcgcgga
     4981 gcgccgcgat ccgcgctggc cggcggcggt cgtgtcgccg tgcgggatct accactgcac
     5041 gaacgcgcgc gatccggacc acgaggccga gctgcgcaag gttctcgggt ccatcgcgaa
     5101 gacgcgtccg acggcggcgg tcgtcgagcc gcacgagcgc ggcgccgcgt gcctcgtgca
     5161 cctgccgggg ttctgcttgc aggcgtgatc tccgtcgcgg gcgcggcgcg ggtggtgggg
     5221 caaattgcct ggtgaatgtt tcagcgtgta tcgccgcacg gctcggggcg cgcgcggttg
     5281 tccgcgccgt gccggcatgc cagattgccc caacccatgg gcggccctgg atcgcgctgg
     5341 ttgcagtcgc ttgtgtcctg tctgctgatc gcgagctgtg gcggctcctc gtcggggccg
     5401 tcgctgctgc cggacgtcac cgacgggggc gggccggtca tggcgcacgt ccagctggtg
     5461 ccgatcttct acgccaacga caccgacgcc gacgcgctga cccgcttcag ccagtggatc
     5521 gtcagctcgc agtggctcaa gacggtgggc gccgactacg gcgtcgatac cggctcggtg
     5581 ctcggggtcg tccaccagcc tggcccggcg cctgccacga tcgacgacag cgacatcgtc
     5641 gccctgctgt acgccggcct ggccaacggc agcctgccca agccggcggg cgggctcgcc
     5701 agcgcgctct acatgatctg gttccccgcc agcaccacgg tgaccgcggg cctcgacaag
     5761 agctgcgatg tgttcggcgg ctaccacaac tcggtgcggc agggcggggt ggagctgtcc
     5821 tacgcggtga tcgcggcgtg ctcgggcttc gtcgacgggc tgagcgatct cgaggaccgc
     5881 gaggtcgtgg cgtcgcacga gctgatcgag gccgcgaccg acccgctccc ggacaaccac
     5941 ccgagcttcc agctcgacga tccgaccagc tcgtggttcg gcttcggcac cgaggtcggc
     6001 gacctgtgcc agcgcggcga cacgacggag atcgtgcgcg agtccggctt cgtcgcgcag
     6061 cggagctggt cgacctcggc cgcggccgcc gagcacgacc cgtgcgtccc gggcgccacg
     6121 gcggactact tcaacctggc cgtgcagacc cccggggtgc tgcgcatcgc gccgggcggg
     6181 caccaggcgg tcacgctccg cgcctgggcg agcggcgccg cgcgcggcac gacgtggcag
     6241 ctgcagaccg gggcggcgat ggacagcgcg cagacgctga cgctcagcgc cagcatgatc
     6301 aaggacggcg cgaccgtctc gctcgacgtc gcgctgccgc agaccgcgca gatcggcgac
     6361 cagccgcagt tcgtcgtgtt ctcggcgacc agcgacacca actactcgat cctgccgctg
     6421 tacgcggtgg ccggggagcc gtgctcgcag ttcaccacgt gcgaggcgtg caccgccgag
     6481 gtcggctgtg ggttctgcgc gtcgagcggc aagtgcgagg cccagggcgg cgtgacgagc
     6541 tctgcccaga gctcgtgctc gggcggctcg ttcgcgacct ggccgggcag ctgcaagggc
     6601 ttctgcgccg gccacagcgg cagctgcatc gactgttcct cgcagccggg ctgcggctgg
     6661 tgcgccagcg ggcagcagca ctgcgtggag gccagtcacg agaccggcca gccggagtcc
     6721 ggcagctgcc cgtacgccga ctggagcttc acgccggact actgctcgca gtagcgcgcc
     6781 tgtcgtcggc tcgacacggc cgtcgaagac cggacagttc ctgacgccgc aatggggcag
     6841 tatgccccga tccggtcgga ccggtgcgcg gcatgcgggt tgtaacacct gccgccatgc
     6901 gcctcgtggt cgtcgtcgcc gtcgtcgccg tcatcggact cgcccgcacc gccgcgttcg
     6961 cggatccgcc cggcatgacg ccgccggtcc cgccggcgcc ccaggatccg gtcgtcgcgc
     7021 cgtattacgg gctgacgctg gccgccgatg gggtggccct ggtcacggcg atggccgcgc
     7081 tgggcggcgg cgactccgag tcgcgcacca ggctgttcca gatcgccgcc ggtacctacg
     7141 tgctcggcgg gccgctcctc cacgtcgtcc acggccggcc ggggcgcgcg gcgatcagcc
     7201 tcgcgatgcg cgtcggcctg ccggtcgtcg ccgccgggct gctctcgtcg ggccacgacg
     7261 gctgggacgc gctggttccg ttcctgctcg gcgcggtcgg cggcacgctc accgcgagca
     7321 tcgtcgacgc cgcggtgctc gcccgg
//
LOCUS       DASVOR010000600         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_239234,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000600 DASVOR010000000
VERSION     DASVOR010000600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239234"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..571
                     /locus_tag="VF516_11880"
     CDS             <1..571
                     /locus_tag="VF516_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108423.1"
                     /translation="EPALARPARDAGAPPESMSDAVSDPTPGAVPEAVPDAELEVDDL
                     DDAADSPAAPAPRHLHERLRGLTLAAQIKLAASGELHERIVLERLYGKNVWETLLRNP
                     RLTPPEVSRIARYGSLPRVLLEIILGNATWLQVPEVRRALLANPRLGTDQILKVLRLT
                     PKHELRLAAIQTVYPHAVRQAARLLLRGD"
     gene            621..1322
                     /gene="rph"
                     /locus_tag="VF516_11885"
     CDS             621..1322
                     /gene="rph"
                     /locus_tag="VF516_11885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEX8108424.1"
                     /translation="MRPDGRRPDQLRALEIIPHYQKHAEGSALIKLGDTWVVCSASVE
                     DGVPGFLAGKGRGWLTAEYAMLPRSTHTRTKRDPGGRGKEIQRLIGRSLRAAVDLDQL
                     GERTIAVDCDVISADGGTRVASITGAWIALAFAIQKLVATGKLADARALRPPVAAVSV
                     GVVGGELVLDLAYAEDSAAEVDMNVVGSEDGSLIEVQGTAEHGTFDRRQLDAMLDLAA
                     AGIRQLAAAQRRVVA"
     gene            1319..>1720
                     /locus_tag="VF516_11890"
     CDS             1319..>1720
                     /locus_tag="VF516_11890"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX8108425.1"
                     /translation="MRPLVFATRNPGKLIELRQLLPGAAVLGIDEAAARLGRAIPEVD
                     EDADTFAGNAAKKAREVSRATGLPALADDSGLCVDALGGRPGVLSARYAEGDDRARWQ
                     KLLRELEGRTDRAAAFVCALGLALPGEEAVVV"
BASE COUNT          218 a          661 c          626 g          215 t
ORIGIN      
        1 ggagcccgcc ctcgctcgtc ccgcccgcga tgccggcgcg ccgccggagt cgatgtccga
       61 cgcggtgtcc gacccgacgc cgggcgcggt ccccgaggcg gtccccgacg ccgagctcga
      121 ggtcgacgac ctcgacgacg cggccgactc ccccgccgcg cctgccccgc gccacctcca
      181 cgagcgcctg cgcggtttga cgctcgcggc gcagatcaag ctcgcggcca gcggcgagct
      241 ccacgagcgg atcgtgctgg agcggctgta cggcaagaac gtctgggaga ccctgctgcg
      301 caacccgcgg ctcacccctc cggaggtatc gcggatcgcg cgctacggct cgctgccgcg
      361 cgtcctgctc gagatcatcc tcggcaacgc gacctggctg caggtccccg aggtccgccg
      421 cgcgctgctg gccaacccgc ggctggggac cgaccagatc ctcaaggtgc tgcgcctgac
      481 ccccaagcac gagctccggc tcgccgcgat ccagaccgtg tacccgcacg cggtgcgcca
      541 ggccgccagg ctgctgctgc gcggcgattg acccggttcg ctcgctcacc ggcggcgcct
      601 ggggtagcct gcgcgccacc atgcggcccg atggcagacg ccccgaccag ctccgcgcgc
      661 tggagatcat cccgcactac cagaagcacg ccgagggctc ggccctgatc aagctcggcg
      721 acacctgggt cgtgtgctcg gcgagcgtcg aggatggcgt gccgggcttc ctcgccggca
      781 agggccgggg ctggctcacc gccgagtacg cgatgctgcc gcgctcgacc cacacccgca
      841 ccaagcgcga ccccggcggc cgcggcaagg agatccagcg gctgatcggt cgctcgctgc
      901 gcgccgcggt cgacctcgac cagctcggcg agcgcacgat cgcggtcgac tgcgacgtga
      961 tctcggccga cggcggcacc cgggtcgcgt cgatcaccgg cgcgtggatc gccctggcgt
     1021 tcgcgatcca gaagctggtc gcgaccggaa agctcgccga cgcgcgcgcg ctgcgcccgc
     1081 cggtcgccgc ggtgtcggtc ggcgtcgtcg gcggcgagct cgtgctcgat ctggcgtacg
     1141 ccgaggactc cgccgccgag gtcgacatga acgtggtcgg cagcgaggac ggctcgctga
     1201 tcgaggtcca gggcaccgcg gagcacggca cgttcgatcg caggcagctc gacgcgatgc
     1261 tcgacctcgc cgcggcgggc atccggcagc tcgcggcggc gcagcgccgg gtggtcgcgt
     1321 gaggccgctg gtgttcgcga cccgcaaccc cggcaagctg atcgagctgc gccagctgtt
     1381 gcccggcgcc gcggtgctcg gcatcgacga ggccgccgcc cggctcggcc gcgcgatccc
     1441 cgaggtcgac gaggacgccg acacgttcgc cggcaacgcg gccaagaagg cgcgcgaggt
     1501 gtcgcgggcg accgggctgc cggcgctcgc cgacgactcg ggcctgtgcg tcgacgcgct
     1561 cggcggccgg cccggggtgc tctcggcccg ctatgccgaa ggcgacgatc gcgcccgctg
     1621 gcagaagctg ctccgcgagc tcgagggacg gaccgatcgc gcggccgcgt tcgtctgcgc
     1681 gctcggcctc gcgctcccgg gcgaagaggc ggtcgtggtc
//
LOCUS       DASVOR010000601         7163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_268457,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000601 DASVOR010000000
VERSION     DASVOR010000601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7163
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_268457"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="VF516_11895"
     CDS             complement(<1..487)
                     /locus_tag="VF516_11895"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX8108426.1"
                     /translation="MSGHHELLRPSGTSFFCDPQIFDRIATTLVPELLAKKPHDEPLR
                     AWCAGCTTGEEAYSLAVVLVEELEARGLTRPIQVFATDPDGGAIAHARHGVFDADIAD
                     AVGSERLARFFHAFSTGYRVNQPLRNKVIFAPHDLARDAPFARIDLILCRGVLTSFER
                     AL"
     gene            complement(862..2580)
                     /locus_tag="VF516_11900"
     CDS             complement(862..2580)
                     /locus_tag="VF516_11900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108427.1"
                     /translation="MNLVPLFRHVGLGLPALLLVGCTGPDGQTVLPSGSTVVARDGGD
                     RGVGLTGVASPVARAAGLTAPSALSPELIAVVAAQGAIAVENPTVLPVGDPAVSVTHY
                     GYDSDGPLLPAPGDVQTAAHQVEATKTEPDKNTYLVLRNQTGADPDYDYGTHFVFQGH
                     ENGARGAGTITRINLDADAAHRVTVMAVADTTGQPLLPIDGSTWDPFAQRLLFTVEAG
                     PLGRVYQATLDVPSAAEDLAGSFGRGGYEEITNDARGNVWLMEDVSGAKGTVHKNARQ
                     PNSFVYRFLPVRADDLHRGKLQALQVLSLRTGQPIAFHAGQADADILSADVGDLHSYG
                     KVFQTRWVTLHDTERDGTAPFDANALAKAGLATPFKRPENGAFRPGSQFREFVFDETG
                     DTDLRTEAGAAFGGFGAILSLTQSAPDADHGQLRMVFRGDPAHTGLDNCAFWDADHVV
                     FVEDAGDGLHGQRNALDSAYLFDLRADYSRPDHPPLRIFAEGRDPSATIDSALGGSPG
                     FQNEGDNEITGFHISDGDPTPRGVLGARLPQPLHRGWRVFFTQQHGDNTTWELLSRPA
                     ADRDDD"
     gene            2943..3770
                     /locus_tag="VF516_11905"
     CDS             2943..3770
                     /locus_tag="VF516_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108428.1"
                     /translation="MIVWIVWSLVGVVAAVGVVIAVARILRNRSTPPLDPTPKVAHVT
                     PLALTPQQIAQFPRLPPVVHHLYAIKPEDGWPPKLARAMAPRTLTPLRPPAVLDRRRE
                     ARLRHLALQDPRVKALLGGRWEYLGAHQTEQRNYRHDGALHVRLSFYNYTARHAIDVQ
                     VVNDDIVEARRLDDYMPPEAPSEIRQAIAMARADARLKGKVEALDSHAILQSPVDPAH
                     PLRGRRILWVVFSEPDDPRREMPARFTALVDLTEQNVVSAGEAPCRSWSRSRSWRER"
     gene            3767..5143
                     /locus_tag="VF516_11910"
     CDS             3767..5143
                     /locus_tag="VF516_11910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108429.1"
                     /translation="MTTDDVRSGHLNWEKWCFDYLVSSTEGLALLNGYHDGRKVFKKL
                     SLPVIRVKYVKDGSAIPDNPFSDGCGPYADHVQWITAESLRGRWTDLVGAPRHLVRIA
                     RCNDRYVGISTFEIPSDEGHSRWLEIGVYARIGAYHIYQVYYLSERLLCPRVFSRGLE
                     CNLEHVHHAYWRLDLDVDGDAPQRVMVVRDGFAGYFDLEGAYRMDGDLTTRWAVENLR
                     TRSRVWIFPDDTPPGEAVGSTDAFCVLDANIRRYRASEDIAWPFHTGELDFQPQDNPD
                     GSDVVFWFVSHLKHAPEQGADPWHAVGPTLLVEPVISSEPQLTEALRAVAVEVEVTVQ
                     PAAALAADPERAFSFSFTQTAALDRDHYHAEIAYQTQPVGGATRAALVATLIWNADGS
                     VGADCSATLYRHTDSVDTEAHHVNVLRDHGEQVNIDLASGGLVPDRARIRLTLTNRHR
                     GALAVLPR"
     gene            complement(5195..6139)
                     /locus_tag="VF516_11915"
                     /pseudo
     CDS             complement(5195..6139)
                     /locus_tag="VF516_11915"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6115..6465)
                     /locus_tag="VF516_11920"
     CDS             complement(6115..6465)
                     /locus_tag="VF516_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108430.1"
                     /translation="MPYLGEMPIRLSALFVLALSGCGFQSDPAPDAAPALSTTEQAIS
                     CSYWKYICDPVDIWANWYCEQACSGSGHCMDYSPMEIAWCAAHPDKFYNPYKLCGPSG
                     DPLWQTWCLPGPTP"
     gene            complement(6642..>7163)
                     /locus_tag="VF516_11925"
     CDS             complement(6642..>7163)
                     /locus_tag="VF516_11925"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108431.1"
                     /translation="VWSRTAANAQRFAAESPVPVEVMPTARAAVDGADLICTTTAARE
                     PILEGAWLAPGAHVNAVGACFATSRELDTAAVARARLVVDRRESALNESGDFLLARRD
                     GAIGDDHILGELGDLLLGRFAGRRAADDVTLFESLGIAIEDLASAHYIYTQALAQGLG
                     ISAPLGPDPQPHP"
BASE COUNT         1111 a         2438 c         2535 g         1079 t
ORIGIN      
        1 gcagcgctcg ctcgaacgag gtcagcacgc cgcggcacag gatcagatcg atccgcgcga
       61 acggcgcatc gcgcgcgagg tcgtgcggcg cgaagatcac cttgttgcgc agcggctggt
      121 tgacccggta gccggtcgag aacgcgtgga agaaccgggc aagccgctcg ctgccgaccg
      181 cgtccgcgat gtccgcgtcg aacacgccgt ggcgggcgtg ggcgatcgcc ccaccgtcgg
      241 ggtcggtcgc gaacacctgg atcggccgcg tcagcccgcg cgcctcgagc tcctcgacga
      301 gcaccaccgc gagcgagtac gcttcctcac cggtcgtaca gccagcgcac caggcacgga
      361 gcggctcgtc gtgtggcttc tttgccagca gctcgggcac gagtgtcgtc gcgatccggt
      421 cgaagatttg gggatcgcaa aagaagctcg ttcccgacgg gcgcaacagc tcgtgatggc
      481 cgctcatggt gaggggcaac tataagtccg gaccgcacgg cggacgagca ttgcaactca
      541 gtgttcattc gattctagac cacgcgcgcc agcgcccggt ccgggtcggt ggcgctgcgc
      601 gactgcgtca cggtctctgc gcggtagcgc gcgatcaggc ggtggccagc gcacggcgtg
      661 cgtgcgcgcg aagatccagg cggcgagcaa caggtccccg agcgagcttg tcaccgaacc
      721 ggcgctgcag atcgcgggca tcgagcgccg gagccacggc cggagccgcg ggggccaggg
      781 gagcagaggc gggcaccgcg aggcgcatgc aagccgctgt gcaccggcga tgccaacgtt
      841 ctgcgagaac atctggcgcg gtcagtcgtc gtcgcgatcg gcggccgggc gcgacagcag
      901 ctcccaggtc gtgttgtcgc cgtgctgctg ggtgaagaac acgcgccagc cgcggtgcag
      961 cggctgcggc agcctggcgc cgagcacgcc gcgcggcgtc ggatcgccgt cggagatgtg
     1021 gaacccggtg atctcgttgt cgccctcgtt ctggaagccc gggctgccgc cgagcgccga
     1081 gtcgatcgtc gccgacggat cgcggccctc ggcgaagatc cgcagcggcg ggtggtccgg
     1141 gcggctgtag tcggcgcgca ggtcgaacag gtacgccgag tcgagcgcgt tgcgctggcc
     1201 gtgcagcccg tcgccggcgt cctcgacgaa caccacgtgg tcggcgtccc agaacgcgca
     1261 gttgtcgagc ccggtgtgcg ccggatcacc gcggaacacc atgcgcagct ggccgtggtc
     1321 ggcgtcgggc gcgctctggg tcagcgacag gatcgcgccg aacccgccga acgccgcgcc
     1381 ggcctcggtg cgcaggtcgg tgtcgccggt ctcgtcgaac acgaactcgc ggaactgcga
     1441 gcccggccgg aacgcgccgt tctccggccg cttgaacggc gtcgcgagcc cggccttggc
     1501 cagcgcgttg gcgtcgaacg gcgccgtgcc gtcgcgctcg gtgtcgtgca gcgtcaccca
     1561 gcgggtctgg aacaccttgc cgtagctgtg caggtcgccg acgtcggcgg acaggatgtc
     1621 ggcgtcggcc tgcccggcgt ggaacgcgat cggctgcccg gtgcgcagcg agagcacctg
     1681 cagcgcctgg agcttgccgc ggtgcaggtc gtccgcgcgg accggcagga agcggtacac
     1741 gaagctgttg ggctggcgcg cgttcttgtg gaccgtgccc ttggcgccgc tgacgtcctc
     1801 catcagccac acgttgccgc gcgcgtcgtt ggtgatctcc tcgtagccgc cgcggccgaa
     1861 cgagccggcc aggtcctcgg ccgccgacgg gacgtcgagc gtggcctggt acacccggcc
     1921 gagcgggccg gcctcgacgg tgaacagcag gcgctgggcg aacgggtccc aggtcgagcc
     1981 gtcgatcggc aacagcggct ggccagtcgt gtcggcgacc gccatcaccg tcacccggtg
     2041 cgcggcgtcg gcgtcgaggt tgatccgcgt gatcgtgccg gccccccgcg cgccgttctc
     2101 gtggccctgg aacacgaagt gcgtgccgta gtcgtagtcg gggtccgcgc cggtctggtt
     2161 gcgcagcacc agataggtgt tcttgtccgg ctcggtcttg gtcgcctcga cctggtgggc
     2221 cgcggtctgg acgtcgcccg gcgccgggag cagcggcccg tcgctgtcgt agccgtagtg
     2281 ggtgaccgac accgccggat cgccgaccgg cagcaccgtc gggttctcga ctgcgatggc
     2341 gccctgggcc gcgaccaccg cgatcagctc gggcgacagc gcgctgggcg cggtgagccc
     2401 cgcggccctg gccaccgggc tcgcgacgcc ggtgaggccc acgccgcggt cgccgccgtc
     2461 gcgcgcgacg acggtcgatc cggagggcag cacggtctgg ccgtcggggc cggtgcaacc
     2521 gacaaggagg agggccggca agccaaggcc gacatggcgg aacagaggta cgaggttcat
     2581 gcgggtctcc tgtggtgtgg gacccttccg tacgaagtgg cggtgagccg cgcgacgcag
     2641 cccggtgacg gcgctgcaac gaatctcgaa cgggcagaat cagatcggtc atcgtgatca
     2701 ccccatgatc cagcgatgaa cctcgcctcg ctcgggcacg ggattgccct cccatcggcg
     2761 ctgtcctccg cggctctgca tcgtgcaccg ggcgccagcg ccacagcgat cgcgagatcc
     2821 tcgggatcca cgccgtgtgg tacgaccgcg catccgcatg cagtgcacgt gattggccgc
     2881 tctggtctgc ttgacgccga cgcgggatat gaaatagtgg aacgttgctc tagcggcgga
     2941 gtgtgatcgt gtggattgtc tggagcctgg tcggcgtcgt ggcggcggtc ggcgtggtca
     3001 tcgcggttgc gcggatcctg cggaatcggt caactccgcc gctcgatccc acacccaagg
     3061 tggcgcacgt gaccccgctt gcgctgacac cgcagcagat cgcgcagttt ccgcgattgc
     3121 caccggtggt acaccacctc tacgcgatca aacccgagga tggctggccg ccgaagctgg
     3181 cccgggcgat ggcgccgcgc accctgaccc cgctgcggcc gccggcggtg ctcgaccgca
     3241 ggcgcgaggc tcggctgcgt cacctcgcgc tgcaagaccc gcgggtcaag gcactgctcg
     3301 gtggccgctg ggagtacctc ggcgctcacc agaccgaaca gcgcaactac cgccatgacg
     3361 gcgcgctcca cgtgcggctg tcgttctaca actacacggc gagacacgcg atcgacgtcc
     3421 aggtggtgaa tgacgacatc gtcgaggctc gccggctcga cgattacatg ccgccggagg
     3481 cgccgagcga gatccgacag gcgatcgcca tggcgcgcgc cgacgcccgc ctgaagggca
     3541 aggtcgaggc gctcgactcg cacgcgatcc tccagagccc cgtggacccc gcacatccgc
     3601 tccggggccg gcggatcctg tgggtggtgt tctcggagcc ggacgatccc cggcgcgaga
     3661 tgccggcgcg tttcaccgcc ctggtcgatc tgaccgagca gaacgtcgtg tcggccggtg
     3721 aggcgccgtg tcggagctgg agtcggagcc ggagttggag agagcgatga ccacggacga
     3781 tgtgcgcagc ggccacctca actgggagaa gtggtgcttc gactatctgg tgtccagcac
     3841 cgagggcctc gcgctgctga acgggtatca cgacggccgg aaggtattca agaagctgag
     3901 cctcccggtg atccgggtca agtacgtcaa ggacggctcc gccatccccg acaacccgtt
     3961 ctccgacggc tgcggcccgt acgccgatca tgtccagtgg atcaccgccg agagcctgcg
     4021 cgggcggtgg accgacctcg tgggggctcc gcggcacctg gtccggatcg cacggtgtaa
     4081 cgacaggtat gttggaatct ccacgttcga gattccgtcc gacgagggtc attcgcgctg
     4141 gctcgagatc ggcgtgtatg cccggatcgg cgcgtaccat atttatcagg tctactatct
     4201 gagcgagcgg ctgctgtgcc cacgggtgtt cagccgcgga ctggagtgca acctcgagca
     4261 cgtccaccac gcgtactggc ggctcgatct cgacgtcgac ggcgacgctc cgcagcgcgt
     4321 gatggtggtg cgcgacggct tcgccgggta cttcgacctc gagggcgcgt accgcatgga
     4381 tggcgacctg accacgcggt gggcggtcga gaacctgcgc acccggagcc gggtgtggat
     4441 ctttcccgac gacaccccgc cgggcgaggc ggtcggcagc accgacgcgt tctgcgtgct
     4501 cgacgccaac atccgcaggt atcgcgcgag cgaggacatc gcctggccgt tccacaccgg
     4561 cgagctcgat ttccagcccc aggacaatcc cgacggcagc gacgtcgtgt tctggttcgt
     4621 ctcgcacctg aagcacgctc ccgagcaggg cgccgaccca tggcacgcgg tcgggccgac
     4681 gctgctcgtc gagccggtga tctcgagcga accgcaactc acggaggcgc tgcgcgcggt
     4741 ggccgtcgag gtcgaggtca cggtgcagcc cgcggccgcg ctcgccgcgg accccgagcg
     4801 cgcgttctcg ttctcgttca cccagaccgc cgcgctcgac cgcgaccact accacgccga
     4861 gatcgcgtac cagacccagc ccgtgggcgg cgcgacgcgc gcggccctgg tggcgacgct
     4921 gatctggaac gccgatggca gcgtcggcgc ggactgcagc gcgacgctct accgccacac
     4981 cgacagcgtg gacaccgagg cgcatcacgt caacgtgcta cgcgatcacg gcgagcaggt
     5041 gaacatcgac ctggccagcg gcggcctggt gccggatcgc gcgcggatcc ggctgacgct
     5101 gaccaaccgg caccgcggcg cgctcgcggt gcttcctcgt taaccagggc ccatgaatcc
     5161 cgggaccgat cgtggtcctg ccccagacac gagcggtgcc atcccaggaa gcggcgacga
     5221 cctgccgagc actcggatcg aagtgtgcga ccatgaccac gttgttcggg ccatcaagca
     5281 tcgagaccaa cattccgtga gccacatctg tgacggcgac tttgccgctg gcgcctgctg
     5341 cgacgacgag cccggaggtc ggatcgaact cgatcgagag gatcttgccg tgcaggtaat
     5401 tgccctggct ccgtagcttt ccggaagccg catcccacac ctgttccgca ccatcctcgc
     5461 tggctgcaac gacgatctcg ccactgggtg agaacgcaac ccgattcact ggctccccga
     5521 cgtcgcgcaa gtgctgaagc cgcgtaccgg tcgggaacgc ccagatcgac acctcgccgt
     5581 ccggggtacc ggtcaagagg cgcgggccgg tcggatccca gctcaggcga tggtggccgg
     5641 cgatcgcgcg aacctgcgcc cacgtccggg tgtcgaacac gcgtacgtca ttgtcgccgc
     5701 tggtcgccag ccagccgccg tcggcgctga acgccaggct gaaatagtcc gctccgcccg
     5761 taggtagctc ggcaagcgcc gcccctttcg tcgcgtccca gacgtgcgcc acctggccct
     5821 tgaagtccat ggccgcgatg agctttccat ctggcagcgc cgccactgcc ccatagcgtc
     5881 gcttgccgtc ctgcctcagc tcgcgcacga gggtcccatt cgatgcatcc cagaccctga
     5941 cggcgccgtc ccccgccgcc gtgaccagcc tcgtgccatg gttgcagtac acggcctgat
     6001 acaccacatc gctgccactc gtcgagagcg tcctcgtcgg tggatcgagg ccgagatctg
     6061 tatcagtgac atgagctgtc tcgtggcgcg tggttgcggt gtcccgtcga cgcctcacgg
     6121 cgttggcccc gggagacacc aggtctgcca gagcgggtct cccgacggac cgcagagctt
     6181 gtaaggatta tagaacttgt ccgggtgtgc ggcgcaccag gcgatctcca tgggcgagta
     6241 gtccatgcag tgccccgacc cgctgcacgc ctgctcgcaa taccagttcg cccagatatc
     6301 gactggatcg cagatgtatt tccagtagct gcaggaaatg gcctgttcgg tggtagacag
     6361 cgccggggcg gcatcgggcg cgggatcgga ttggaacccg cagccggata gagcaagcac
     6421 gaacagagcg gatagacgga tcggcatttc accgaggtac ggcacgtgga gcagtacgtc
     6481 aacgcgagca ttctctcata caggacgtgg gcgcatgcag caggacgcca ctactgagcg
     6541 cccacgagac accgtggcac ggagatcgat caccgcgccg cgtctcgtgg cggggatcga
     6601 cggagggagg cgcgcccgcg agcaccgcgg cggcgcgtcg atcaaggatg cggctgagga
     6661 tccgggccga gcggcgccga gatgcccagg ccctgggcga gcgcctgggt atagatgtag
     6721 tgcgccgagg cgaggtcctc gatcgcgatg ccgagcgact cgaacagcgt gacgtcgtcg
     6781 gcggcgcgcc ggccggcgaa ccggccgagc agcagatcgc cgagctcgcc gaggatgtgg
     6841 tcgtcgccga tcgcgccgtc gcgcctggcg agcaagaagt cgccggactc gttgagcgcc
     6901 gactccctgc gatcgacgac gaggcgggcg cgggcgaccg cggcggtgtc gagctcgcgg
     6961 ctggtggcga agcacgcgcc gacggcgttg acgtgggcgc cgggggcgag ccatgcgccc
     7021 tcgaggatcg gctcgcgggc ggcggtggtg gtgcagatca ggtcggcgcc gtcgaccgcg
     7081 gcgcgcgcgg tgggcatgac ctcgaccggc accgggctct cggcggcgaa ccgctgggcg
     7141 ttggccgcgg tacggctcca gac
//
LOCUS       DASVOR010000602         2398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_385412,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000602 DASVOR010000000
VERSION     DASVOR010000602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385412"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1138
                     /locus_tag="VF516_11930"
     CDS             137..1138
                     /locus_tag="VF516_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108432.1"
                     /translation="MHSVRCHALLSLAIAGLAACGGRPPAPASDAPPSTIPTSPVGVF
                     AMTSTLDFHVPAAAAPALQALLAATDGPDDPARFVVDRLVALLPDGSVKTIADAAAPY
                     VAAYVNARLADLAPRLATGLDGLATGLARIATHLGTVETLQIDGRGTGVRTITGVRFE
                     VGNAVTVVPFAAAGLTDLAAGVRITLDGSGRIAISEHEHALPYGAILRLGLDRAVAPS
                     VAPTAGDLAGALGALLDCDQLGIVIANRVGLGSATLYSATCRAAMTALAAEVDAQIAA
                     IDRTPLGIEVAGTAMGYDGNGDGVMDELDAGRWTGSVYSGSDREPIDAASFVSETSR"
     gene            1219..1677
                     /locus_tag="VF516_11935"
     CDS             1219..1677
                     /locus_tag="VF516_11935"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8108433.1"
                     /translation="MTDPVQELVHDHADINRRVLALGAAIRALDRPDGKGMAVALVHQ
                     LGELRELLFLHFAREEEGLFPFVSESVPDLADRVQSMAMAHDAICGGLARMDHLASAN
                     VELAQVLAVFARFEAGYASHADAEAELLRELTARLDAEQRARLADLVHGL"
     gene            1680..>2398
                     /locus_tag="VF516_11940"
     CDS             1680..>2398
                     /locus_tag="VF516_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8108434.1"
                     /translation="MIELIEVSKRYGAPGAPWAVHGLSLAVAAGELVALLGESGSGKT
                     TLLKLVNRLLDPDLGAIRVAGRDIRGENPVALRRTIGYVIQHAGLLPHLSVADNVAMV
                     PRLLGWRRGDIAPRVDELLALVGLPPDEFRARFPDQLSGGQRQRVGVARALAARPKLV
                     LLDEPFGALDPITRAALQGELARIHRELGLTTLLVTHDLTEALTLADRIVVMRAGEVR
                     QIATPRQLITAPADGYVASLV"
BASE COUNT          332 a          845 c          877 g          344 t
ORIGIN      
        1 tcgcagtgag aacgacggcg cggtcgatcc tcgacgaaga tcgactcgaa caacggatcg
       61 tgctgcggca cgacgtgcaa cggcacggtc ggtgcatcgc ggcccggcaa ccacttgccg
      121 gagacctgtc atgcccatgc attccgttcg ctgccacgca ctcttgtcac tcgcgatcgc
      181 cgggctcgcc gcctgcggag gccggccgcc cgcgcccgcc agtgacgcgc caccgtcgac
      241 cataccgacc agcccggtcg gagtgttcgc gatgacgagc acgctcgact ttcacgtgcc
      301 cgccgcggca gcgccggcgc tacaggcgtt gctcgcggcc accgatgggc ccgacgaccc
      361 ggcgcggttc gtcgtcgacc ggctcgtcgc gctgctgccg gacggcagcg tgaagacgat
      421 cgcggacgcg gcggcgccgt acgtcgcggc gtacgtcaac gcgcgactcg cggacctcgc
      481 gccgcggctg gcgacggggc tcgacgggct ggcgacgggg ctggcgcgga tcgcaaccca
      541 cctcggcaca gtcgaaaccc tgcagattga cggccgcggc acgggggtcc ggacaattac
      601 cggcgtgcgg ttcgaggtcg gcaatgccgt gacggtcgtt ccgttcgccg cagccgggct
      661 gaccgacctc gcagccgggg tccggatcac gctggacggg agcgggcgga tcgcgatctc
      721 ggagcacgag catgcgctgc cttacggcgc gatcctgcgg ctcgggctcg accgggcggt
      781 ggcgcccagc gtcgcaccga ccgcgggcga tcttgcgggg gcgcttggcg cgctgctcga
      841 ctgcgatcag ctgggcatcg tgatcgccaa ccgcgtcggg ctcggctccg cgacgctgta
      901 cagcgcgaca tgcagggcgg cgatgaccgc actcgctgcc gaagtcgacg cccagatcgc
      961 ggcgatcgat cgaacgccgc tcgggatcga ggtagccggc acggcgatgg gctacgacgg
     1021 caatggcgac ggggtgatgg acgagctcga cgcggggcga tggaccgggt cggtgtactc
     1081 cggctcggat cgcgagccga tcgacgccgc gagcttcgtc agcgagacct cgcggtagcg
     1141 acgagccggc ccggcacggt ggtacagtcg cggcgtctcc gtccctgccg gtcccccgcc
     1201 ggcactcgcc cggccgccat gaccgacccc gtccaggagc tcgtccacga tcacgccgat
     1261 atcaaccgcc gggtgctggc gctcggcgcg gcgatccgcg cgctcgaccg ccccgacggc
     1321 aagggcatgg cggtcgcgct ggtccaccaa ctcggcgagc tgcgggagct gctgttcctg
     1381 cacttcgcgc gcgaagaaga ggggctgttc ccgttcgtgt cggagtcggt gcctgatctg
     1441 gccgacaggg tacagtcgat ggcgatggcg cacgacgcga tctgcggcgg gctggcccgg
     1501 atggatcacc tggcgtcggc caacgtcgag ttggcccagg tccttgcagt gttcgcccgg
     1561 ttcgaggcgg ggtacgccag ccacgccgac gccgaggcag agctcctgcg cgagctgact
     1621 gcgcgactcg atgccgagca gcgcgcgcgg ctggccgacc tggtgcatgg gttgtaggtg
     1681 tgatcgagct gatcgaggtc tccaagcgct acggcgcgcc tggcgcgccg tgggcagtgc
     1741 acgggctgtc gctggcggtg gccgccggcg agctggtcgc gttgctcggg gagtcgggca
     1801 gcggcaagac cacgctgctc aagctggtca accgcctgct cgatcccgac ctgggcgcga
     1861 tccgggtcgc cggccgcgac atccgaggcg agaacccggt cgcgctacgc cgcacgatcg
     1921 gttacgtcat ccagcacgcg ggcctcttgc cccacctgtc ggtcgcggac aatgtcgcga
     1981 tggtgccccg gctgctaggc tggcggcgag gagacattgc gccgcgggtc gacgaactgc
     2041 tcgcgctggt cgggctgccg ccggatgagt tccgagcgcg gttccccgac cagctgtccg
     2101 gcggccagcg ccagcgcgtc ggcgtcgcgc gcgcgctcgc ggcccggccc aagctggtgc
     2161 tgctcgacga gccgttcggc gcgctcgacc cgatcacccg cgcggcgttg cagggtgagc
     2221 tcgcccggat ccaccgcgag ctgggcctga ccacgctgct ggtaacccat gacctgaccg
     2281 aggcgttgac cctggcagac cgcatcgtcg tgatgcgcgc cggggaagtc cgccagatcg
     2341 cgacgccgcg gcagctgatc accgcgccgg ccgacggcta cgtcgcaagc ctggtcga
//
LOCUS       DASVOR010000603         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_210280,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000603 DASVOR010000000
VERSION     DASVOR010000603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210280"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF516_11945"
     CDS             <1..232
                     /locus_tag="VF516_11945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108435.1"
                     /translation="LWRSLIPPEVPIAGKIDFTGLSQRFRLSGGYIRNAALRAAFLAA
                     EEGSALTHAHLERAIRMEFREIGKLAETGTLE"
     gene            complement(319..822)
                     /locus_tag="VF516_11950"
     CDS             complement(319..822)
                     /locus_tag="VF516_11950"
                     /inference="COORDINATES: protein motif:HMM:NF037928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX8108436.1"
                     /translation="MNRTCIAIVDASRARLFTFDRTQGASGLHEQLVEQRDLIDPARR
                     LRPSEQLSDSRPGSSRTGGLQFGFDDHRDAYLDRLDTTFARAVVDEIEALLRSSGARR
                     LILCASPRMLGELRAAGGGLRREGLVIDELPRDLVKLTVPKLRERLGSYNLLPPLPRR
                     TGLRHSA"
     gene            complement(927..1181)
                     /locus_tag="VF516_11955"
     CDS             complement(927..1181)
                     /locus_tag="VF516_11955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108437.1"
                     /translation="MTTATHAQIVELLGDVDDLFAKRIEDTGASLDEIGEALGDLESE
                     DVLGEPLHIPSSPRIAEVRAILEELMDTDEDDLEQGYRQL"
     gene            complement(1319..>1683)
                     /locus_tag="VF516_11960"
     CDS             complement(1319..>1683)
                     /locus_tag="VF516_11960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108438.1"
                     /translation="SHRVGAGVGFGPRSPVAGLEVMGYAAPTMNERAGVAVTAGIGGY
                     SRAFGGGNRSSFNPYVGMRYGYAYLEAHYFVVAAELGVELVKQHGVLWSVSARPIGLI
                     GSDSQAAVEIGSSLALAF"
BASE COUNT          242 a          594 c          607 g          240 t
ORIGIN      
        1 gctgtggcgc tcgctgatcc cgcccgaggt cccgatcgcc ggcaagatcg acttcaccgg
       61 cctgtcgcag cggttccggc tgtcgggcgg ctacatccgc aacgccgccc tgcgcgccgc
      121 gttcctcgcc gccgaggagg gcagcgcctt gacccacgcc cacctcgagc gcgcgatccg
      181 catggagttc cgcgagatcg gcaagctcgc cgagaccggc acgctggagt aggcgcgatc
      241 ccggcggcgc ccaggatgtt catgatctgg aacgcgtgat cggcggcagc ggtcggtcgc
      301 gccggcgagg ccggggtgtc acgccgagtg ccgcaggccg gtgcggcgcg gcagcggcgg
      361 cagcaggttg tacgagccca gccgctcgcg gagcttgggc accgtcagct tgaccagatc
      421 gcgcggtagc tcgtcgatca ccaggccctc gcggcgcagg ccgccgccgg ccgcgcgcag
      481 ctcgccgagc atgcgcgggc tcgcgcacag gatcaggcgg cgcgcacccg acgagcgcag
      541 cagcgcttcg atctcgtcca ccacggcgcg ggcgaacgtc gtgtcgagcc ggtcgaggta
      601 cgcgtcgcgg tgatcgtcga agccgaactg caggccgccg gtgcgactcg accccggccg
      661 gctatcggac agctgctcgg acggccgcag ccggcgcgcc gggtcgatca ggtcgcgctg
      721 ctccacgagc tgctcgtgga ggccgctcgc gccctgcgtg cggtcgaagg tgaacaaccg
      781 ggcccgggaa gcgtcgacga tggcgatgca tgtgcggttc atgcccaggt tcggtgcaag
      841 gcggtggcca tcgtgtacgg gtggtggccg gcccgaaacg cgcaacgtct gcgccgcgga
      901 ccgcgcgtgc cgacggcttt gcgccctcac agctggcggt agccctgctc caggtcgtcc
      961 tcgtcggtgt ccatcagctc ttcgaggatc gcgcggacct cggcgatccg cggtgagctc
     1021 gggatgtgca gcggctcgcc gagcacgtcc tcgctctcca ggtccccgag cgcctcgccg
     1081 atctcgtcga gcgacgcgcc ggtgtcctcg atgcgcttcg cgaacaggtc atcgacatcg
     1141 ccgagcagct cgacgatctg ggcgtgggtg gcggtggtca tgcctggcag gaggtgcaca
     1201 tgccgcgcca gggaaagtcg gcggacctac gaagatcgtt gagcgcgagg atctcgctga
     1261 tcgtcgggta ctcatggttg cgtttcgcct cgccgtgtgc gcgtgcacgt ccacgaggtc
     1321 agaacgcgag cgcgagcgaa gagccgatct ccaccgcggc ctgcgagtca ctgccgatca
     1381 ggccgatcgg ccgcgccgac accgaccaca gcacgccgtg ctgcttgacg agctcgacgc
     1441 cgagctcggc cgcgaccacg aaataatgcg cctcgaggta cgcgtagccg taccgcatgc
     1501 cgacgtaggg attgaacgag ctgcggttgc cgccgccgaa cgcccgcgag tagccgccga
     1561 tgccggccgt gacggcgacg ccggcgcgct cgttcatcgt cggagccgcg tagcccatga
     1621 cctcgagccc ggcgaccggc gaccggggtc cgaacccgac gccggcgccg acgcgatggc
     1681 tgg
//
LOCUS       DASVOR010000604         7444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_561232,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000604 DASVOR010000000
VERSION     DASVOR010000604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7444
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561232"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VF516_11965"
     CDS             <1..900
                     /locus_tag="VF516_11965"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8108439.1"
                     /translation="IDLAVVPSMTTTTEPFAPDLRGRCFTGARLASASLAGRDVRGAD
                     FTEADLRGADLSHIRAGMSRGWTALLVIGSLALSIALGVVVGLCARFLHAMYTSDEPR
                     RRLVAVFIVAALLAVLIAGIWKGLWFATRHVLPVAAALAIVAGTAGAISGIGTGAGAL
                     LALVFLALATVIVALSVLVRATAGTAGGLYFAMVAIAGGLAGGAMGGGLIAAAVAIGA
                     MLMARRSARLERAFPLLSRVTAAIASRGGTRFRGADLTGVNLEHARLVACDFRGANLR
                     GARLDRATLRLCRFDPVAPPPGA"
     gene            951..1283
                     /locus_tag="VF516_11970"
     CDS             951..1283
                     /locus_tag="VF516_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108440.1"
                     /translation="MSRRILPVALVAAFAAAGCYGTAGYATDGYYGGYYAPYAYGGNY
                     YGYPYYRYRPYYRPYVRYYGYRGYPYYPHPGYRGTWGYRGAPYYHGAPYHGAPYHGAP
                     VHVPHGHR"
     gene            1322..3139
                     /locus_tag="VF516_11975"
     CDS             1322..3139
                     /locus_tag="VF516_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8108441.1"
                     /translation="MASRIENYGLISNMRGAALVARNGSIDWLCLPRFDSDACMAALL
                     GRDEHGCWTIDPAADIRHATRRYRPGTMILETELACVGGRVRIVDFMPLGRNAIVRVV
                     EGLDGSVPVDLNLVARFGYGHYHPWLRQIDGDVVLTSAPDALVLRSSARLDVDEHDVR
                     GVVTVNAGDRLGFELSWFPASQPPPPPLDWRAELERSERLATDWISRSTYRGPYRDAV
                     NRSLITLKAMIYEPSGGIVAAPTAALPEEIGGVRNWDYRFCWLRDATLTLDGLMVGGY
                     TEEARAWRNWLLNAVAGAPDQVQIMYGIDGERRLTEFEVPWLPGYEESRPVRNGNAAS
                     EQFQLDVYGEVLQALYEARRLGLPEEESAIGPGIQLMEFVASAWQLPDDGIWEVRGGR
                     RHFVHSKVMAWVAVDRFVRLVEDFFPRDAALLRLLPRYRALRERIHDDVLEHGFDAQQ
                     NAFVQAYNSSALDASVLLIPALGFLPADDPRVQGTIRAIERKLVRDGFVLRYQPEHTQ
                     DGLAGAEGAFLACSFWLVDAYAYSGRRRDAEALFEHLLSLRNDLGLLSEEFEPRSGRL
                     IGNFPQAFSHLALVHSASVLADAELTPRSRPDARARSVA"
     gene            complement(3154..4278)
                     /locus_tag="VF516_11980"
     CDS             complement(3154..4278)
                     /locus_tag="VF516_11980"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8108442.1"
                     /translation="MRSVKNILWLGFKELRSLLSDAVLVAFVVFAFTWTVYSQATGTS
                     NEVNNASIAFADEDGSTLSQELSNAFFPPRFQNPVPIPTGEIEDAMDRGRFMFVVTIP
                     PDFEHDLRAGRHPDIQLSIDATAMQQAGIGAGYIKNIIAQRISTFLERSDLPSGDAVH
                     LVVRKLFNPNGVSAWFTSVVAIINQLTLLTIVLTGAAVIREREHGTLEHLLVMPLTAF
                     EIAMAKVWANGLVILIATAMSLYLVVQTALDVPFAGSHALWFGGVLLYLFFATALGIF
                     LGTISRSMAQFALLIVLVVVVLQLLSGGSTPVESQPGWLQAVTYFLPTRHFVSFSQVI
                     IYRGGGIAAVWRHFAVVAAVGLAFFVYSISRFRKSIAVTR"
     gene            complement(4281..7085)
                     /gene="rbbA"
                     /locus_tag="VF516_11985"
     CDS             complement(4281..7085)
                     /gene="rbbA"
                     /locus_tag="VF516_11985"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HEX8108443.1"
                     /translation="MATPQPTPSGTAAGTGAAGPGVVPVVVIEDVTHHYGSVVALDHI
                     SVAIPAGLMVGIIGPDGVGKSTLMALVAGSKRLQDGTLTVLGGDIASARHRRTACPRI
                     AYMPQGLGKNLYLELSVHDNVDFMAQLFGLSETERQARVAELLQATGLAPFADRPAGK
                     LSGGMKQKVGLCGALVHDPDLLILDEPTTGVDPLSRRQFWTLIDGIRAGRPGMSVMIS
                     TAYMDEAQQWDWIVAMDAGRVLATGTPAELMRRTGTRDLEQCFIALLPEDKRRGHTEL
                     VIPPRPAGAHEIAIEAQGLTRRFGSFTAVDHVTLSIERGEIFGFLGSNGCGKSTTMKM
                     LTGLLPPTEGTATLFGSSVEAGSLEVRTNLGYMTQAFSLYEELTVRQNLVLDARLYHL
                     PDDQAQARIAMLVERFGLGPHLDALAGDLPMGLRQRLSLAVAVLHGPKLLILDEPTSG
                     VDPVARDSFWELLIDLSRTQGVTIFVTTHFMNEGMRCDRISLMHAGRVLACDAPQALI
                     EQRGVASLEDAFIAYMEDAVADPPAPEAEAARPAPALEQPASARQPRRRLLPLPVRRL
                     LAYSRNEAIQIRRDPVRLAFAFVGSALLMLVFGFGITTDVENVRYAAFDRDHTPESQA
                     YLDQFHGARQYFSPTPPVHSDETGLERMQSGDVSLIVEIPPSLGRDVRRGAGPEVLAQ
                     VDGAMPFRAETVAQYARGVHNDLLQDPGTGLPTGPKRYSAQVQERYMYNPTFESIYSI
                     VPSVPALLLILIPAILMTVSIVREKELGSIINFYVTPTGRLEYLIGKQLPYIAIGMIN
                     FFILAAIAAIVFGVSVKGSFLVLALCTLLYVIVTTGIGMVTSTFTSTQVAAVFVTAIL
                     TIQPTIQFAGLLQPVSTLQGGARLIGTIWPTTYYMHSSVGAYTKGLAPHLMTQDLIVL
                     ACTIPVLLAISMLGLRKQDR"
     gene            complement(7088..>7444)
                     /locus_tag="VF516_11990"
     CDS             complement(7088..>7444)
                     /locus_tag="VF516_11990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108444.1"
                     /translation="FTVDYEPNRVAPGYVSFVSPEAQFTPKEVETRSEREKLMFRVKI
                     QVPRELVLHYVERIKTGVRGVGYVKISDAAVWPDWLQKNLVGPPASPPGRPQASPPVS
                     AIPPSPPPSTPASDLE"
BASE COUNT         1105 a         2528 c         2642 g         1169 t
ORIGIN      
        1 atcgatcttg ctgtcgtgcc gtccatgacc acgaccaccg agcccttcgc gccggacctg
       61 cgcggccgct gcttcaccgg ggctcgcctg gcctcggcat cgctcgcggg gcgggatgtc
      121 cgcggcgcgg acttcaccga ggccgatctg cgcggtgccg atctgtcgca tatccgggcc
      181 gggatgagcc gcggctggac ggcgctgctc gtcatcggat cgctggcgct gtcgatcgcg
      241 ctcggtgtcg tcgtcgggct gtgcgcgcgg ttcctgcacg cgatgtacac gagcgatgaa
      301 ccgaggcgcc ggctggtggc ggtgttcatc gtcgccgcgc tgctcgcggt gctgatcgcc
      361 gggatctgga agggtttgtg gttcgcgacg cgccacgtgc tgccggtggc cgcggcgctc
      421 gcgatcgtgg cgggcacagc cggtgcgatc agcgggatcg ggaccggcgc cggcgcgttg
      481 ctcgccctgg tgtttctcgc gctcgccacc gtcatcgtgg cgctgagcgt cctggtgcgt
      541 gccacggccg gcaccgcggg gggcctgtat tttgcgatgg tcgcgatcgc aggtgggctc
      601 gcaggaggcg cgatgggcgg cggtctcatc gcagccgcgg tcgcgatcgg cgcgatgctc
      661 atggcgcgcc ggtcggcccg gctcgagagg gcgtttccgc tgctgtcgcg ggtcaccgcc
      721 gcgatcgcca gccggggcgg cacgcggttt cgcggtgcgg acctgaccgg cgtcaacctc
      781 gagcacgccc ggctcgtcgc ctgcgacttt cgcggggcca acctgcgcgg ggcccggctc
      841 gatcgcgcga cgctgcggct gtgtcggttc gacccggtcg cgccgccacc cggcgcgtga
      901 cttcgccccg cctggcacag ccgctgcttc gtgatgagtc gaggttcaac atgtctcggc
      961 gcattctccc agtcgcgctc gtcgccgcgt tcgcagcggc gggctgttac ggcaccgccg
     1021 gttacgcgac cgatgggtac tacggagggt actacgcccc ctatgcctac ggcggaaatt
     1081 actatggata tccgtattat cgatatcgtc cgtattatcg tccatacgtt cgctattacg
     1141 gttatcgcgg atatccgtac tatcctcacc cgggctaccg cgggacctgg ggctaccggg
     1201 gggctccgta ctaccacggc gcgccctatc acggcgcgcc ctatcacggc gcgccggtgc
     1261 acgtcccgca cggccaccga tgaccactcc acctgccagc tgacccagga ggtacgaatt
     1321 catggcttct cgcatcgaga actacgggct gatcagcaac atgcgcggcg cggcgctggt
     1381 agcgcgcaat ggatcgattg actggttatg tttgcctcgg ttcgattctg atgcgtgcat
     1441 ggccgcgctg ctcggtcgcg acgagcacgg ctgctggacg atcgatccgg ccgccgacat
     1501 ccgccacgcg acgcgccggt atcgccccgg cacgatgatc ctcgagaccg agctcgcgtg
     1561 cgtcggcggc cgggtccgta tcgtcgactt catgccgctc gggcgcaacg cgatcgtccg
     1621 cgtcgtcgag gggctcgatg gctcggtccc ggtcgacctc aacctcgtgg cgcggttcgg
     1681 ctacggtcac taccatccgt ggctgcggca gatcgatgga gacgtcgtgc tgacatcggc
     1741 gcccgatgcg ctcgtgctcc gcagctccgc gcggctcgac gtcgacgagc acgatgtccg
     1801 cggcgtggtc accgtgaacg cgggggatcg cctcgggttc gagctgagct ggttcccggc
     1861 cagccagcca ccgccgccgc cgctggactg gcgggccgag ctcgagcgca gcgagcgcct
     1921 cgcgaccgac tggatctcgc gctcgaccta ccgcggcccg tatcgcgatg cggtgaaccg
     1981 ctcgctgatc acgctcaagg cgatgatcta cgagccgagc gggggcatcg tggcggcgcc
     2041 gaccgcggcg ctccccgagg agatcggcgg tgtgcgcaac tgggactacc ggttctgctg
     2101 gctgcgcgat gccacgctga cgctcgatgg cctgatggtc ggcggctaca ccgaggaggc
     2161 gagggcgtgg cgaaactggc tgctcaacgc cgtggccggc gcgcccgacc aggtccagat
     2221 catgtacggg atcgacggcg aacgccgcct caccgagttc gaggtgccgt ggcttcccgg
     2281 gtacgaggag tcccggccgg tgcgcaacgg caacgcggcg agcgagcagt tccagctcga
     2341 cgtctacggc gaggtcctgc aggcgctcta cgaggcgcgc cggctcggcc tcccggagga
     2401 ggaatcggcg atcggccccg ggatccagct gatggagttc gtggcgtcag cctggcagct
     2461 tcccgatgac gggatctggg aggtgcgcgg cggccggcgc cacttcgtgc actcgaaggt
     2521 gatggcgtgg gtcgcggtgg atcgcttcgt ccggctggtc gaggacttct tcccccgcga
     2581 cgcggccctg ctccggctgc tgccgcgcta ccgcgcgctg cgcgagcgca tccacgatga
     2641 cgtcctcgag cacggcttcg acgcccagca gaacgcgttc gtgcaggcct acaactccag
     2701 cgcgctcgac gcgagcgtgc tgctgatccc ggccctcggg ttcctgcccg cggatgaccc
     2761 gcgcgtgcag gggacgatcc gggcgatcga gcgcaagctc gtgcgcgacg gcttcgtcct
     2821 gcgctaccag cccgagcaca cccaggacgg cctcgccggt gcagagggcg cgttcctcgc
     2881 atgcagcttc tggctggtcg acgcctacgc gtactccggc cgccgccgcg acgccgaggc
     2941 gctgttcgag cacctgctgt cgctgcgcaa cgacctcggc ctcttgtccg aggagttcga
     3001 gccacgctcc ggccggctga tcggcaactt cccgcaggcg ttctcgcacc tcgcgctggt
     3061 ccactcggcg agcgtgctcg cggacgcgga gctcacgccg cgctcgcggc cggacgctcg
     3121 tgcgcgctcg gtcgcgtgac gcggctcgcc gcgctacctg gtcaccgcga tcgacttccg
     3181 gaaccgcgag atgctgtaca cgaagaacgc caggccgacg gcagccacca ccgcgaagtg
     3241 gcgccacacc gcggcgatgc cgccgccgcg gtagatgatc acctgcgaga agctgacgaa
     3301 gtggcgggtc ggcaggaagt aggtgacggc ctgcagccat cccggctggc tctcgaccgg
     3361 ggtcgatccg cccgacagca gctgcagcac gacgacgacg aggacgatca ggagcgcgaa
     3421 ctgcgccatc gagcgcgaga tcgtgccgag gaagatcccg agcgcggtcg cgaagaacag
     3481 gtagagcagg acgccgccga accacagcgc gtgcgagccg gcgaacggca cgtcgagcgc
     3541 ggtctggacg accaggtaca gcgacatcgc ggtcgcgatc aggatcacca ggccgttggc
     3601 ccacaccttg gccatcgcga tctcgaacgc ggtcagcggc atcaccagga ggtgctcgag
     3661 cgtcccgtgc tcgcgctcgc ggatcaccgc ggcgccggtc agcacgatgg tcagcagggt
     3721 cagctggttg atgatggcga ccacgctggt gaaccacgcc gacaccccgt tgggattgaa
     3781 cagcttgcgc acgaccaggt ggacggcgtc gcccgacggg aggtccgagc gctcgaggaa
     3841 cgtcgagatc cgctgggcga tgatgttctt gatgtagccg gccccgatcc cggcctgctg
     3901 catggcggtc gcgtcgatgc tgagctggat gtcgggatgg cgtccggcgc gcaggtcatg
     3961 ctcgaagtcg ggagggatcg tgaccacgaa catgaaccgc ccgcggtcca tcgcgtcctc
     4021 gatctcgccg gtcgggatcg gcaccggatt ctggaaccgc ggcgggaaga acgcattgga
     4081 cagctcctgc gacagcgtgg agccatcctc gtcggcgaac gcgatcgacg cgttgttgac
     4141 ctcgttcgag gtcccggtcg cctgcgagta caccgtccac gtgaacgcga acacgacgaa
     4201 cgcgaccagc accgcgtcgc tcagcaggct gcgcagctcc ttgaacccga gccacaggat
     4261 gttcttgacc gatcgcacgg ctagcggtcc tgcttgcgga ggccgagcat gctgatcgcc
     4321 agcaggaccg ggatggtgca cgcgagcacg atcaggtcct gcgtcatcag gtggggtgcc
     4381 agacccttgg tgtacgcccc cacgctcgag tgcatgtaat acgtcgtcgg ccagatcgtg
     4441 ccgatcagcc gcgcgccgcc ctggagcgtc gacaccggct gcagcaggcc ggcgaactgg
     4501 atcgtcggct ggatggtcag gatcgcggtg acgaacaccg ccgcgacctg cgtgctggtg
     4561 aacgtcgagg tcaccatgcc gatcccggtc gtcacgatca cgtagagcaa cgtgcacagc
     4621 gccaggacca ggaagctgcc cttgaccgag accccgaaca cgatcgcggc gatcgcggcc
     4681 aggatgaaga agttgatcat gccgatcgcg atgtagggca gctgcttgcc gatcaggtac
     4741 tcgagcctgc ccgtcggcgt gacgtagaag ttgatgatcg agcccagctc cttctcgcgc
     4801 acgatgctga cggtcatcag gatcgcgggg atcaggatca gcagcagcgc cgggacgctc
     4861 ggcacgatcg agtagatgct ctcgaacgtg ggattgtaca tgtagcgctc ctgcacctgg
     4921 gcgctgtacc gcttcgggcc ggtcggcagg ccggtcccgg gatcctgcag caggtcgttg
     4981 tgcacgccgc gcgcgtactg cgcgacggtc tcggcgcgaa acggcatcgc gccgtcgacc
     5041 tgggccagga cctcgggccc ggcgccccgc cgcacgtcgc gccccaagct cggcgggatc
     5101 tcgacgatca gcgagacgtc gcccgactgc atgcgctcga ggccggtttc atcggagtgg
     5161 accggcggcg tcgggctgaa gtactggcgc gcgccgtgga actggtcgag gtaggcctgg
     5221 ctctcggggg tgtgatcgcg atcgaacgcc gcgtagcgca cgttctcgac gtcggtcgtg
     5281 atcccgaagc cgaacacgag catgagcagc gccgagccga cgaacgcgaa cgccaggcga
     5341 accggatcgc ggcggatctg gatcgcctcg ttgcgcgagt aggcgagcaa ccggcgcacc
     5401 ggcagcggga gcaaccgccg gcggggctgt cgcgcggacg ccggctgctc gagcgccggc
     5461 gccggacgcg cggcctcggc ctcgggcgcg ggcgggtcgg cgaccgcgtc ctccatgtag
     5521 gcgatgaacg cgtcctcgag gctcgcgacg ccgcgctgct cgatcagcgc ctgcggcgca
     5581 tcgcaggcca gcacccgccc ggcgtgcatc agcgagatcc gatcgcagcg catcccttcg
     5641 ttcatgaagt gcgtggtcac gaagatcgtc acgccctggg ttcgcgacag gtcgatcagc
     5701 agctcccaga agctgtcgcg ggcgaccgga tcgacgccgg acgtcggctc gtcgaggatc
     5761 aacagcttcg gtccgtgcag gacggcgacc gccagcgaca gccgctggcg cagccccatc
     5821 ggcaggtcgc cggcgagcgc gtcgaggtgc gggccgagcc cgaagcgctc gaccagcatg
     5881 gcgatgcgcg cctgggcctg gtcgtccggc aggtgataca gccgggcgtc gagcaccagg
     5941 ttctggcgga cggtcagctc ctcgtacagc gagaacgcct gggtcatgta cccgaggttg
     6001 gtccggacct cgaggctgcc ggcctcgacc gaggacccga acagcgtcgc ggtcccctcg
     6061 gtcggtggca gcagcccggt cagcatcttc atcgtcgtcg atttgccgca cccgttggag
     6121 ccgaggaagc cgaagatctc gccgcgctcg atcgacagcg tcacgtgatc gacggccgtg
     6181 aagctcccga accggcgggt caacccctgg gcctcgatcg cgatctcgtg ggcgccagcc
     6241 ggccgcggtg gaatgacgag ctcggtgtgc cctctccgct tgtcctccgg caacagcgcg
     6301 atgaagcact gctcgagatc ccgggtgccg gtgcgccgca tcagctccgc gggcgtcccg
     6361 gtggccagca cgcgtccggc gtccatcgcg acgatccagt cccactgctg cgcctcgtcc
     6421 atgtacgcgg tggagatcat cacgctcatg ccggggcggc cggcgcggat cccatcgatc
     6481 agcgtccaga actgccgccg cgacagcgga tcgaccccgg tggtcggctc gtcgaggatg
     6541 agcaggtcgg ggtcgtggac cagcgcgccg cacagcccga ccttctgctt catgccgccc
     6601 gacagcttgc cggcagggcg atcggcaaac ggcgcgagcc cggtggcctg gagcagctcc
     6661 gcgacgcggg cctggcgctc ggtctcggac agcccgaaca gctgggccat gaagtcgacg
     6721 ttgtcgtgga cgctgagctc gaggtagagg ttcttgccca ggccctgggg catgtaggcg
     6781 atccgcgggc acgcggtgcg ccggtgccgg gccgaggcga tgtctccgcc cagcacggtc
     6841 agcgtgccgt cctgcagccg cttggagccg gcgaccaggg ccatgagcgt cgacttgccg
     6901 acgccgtccg ggccgatgat gccgaccatc aggccggcgg gaatcgcgac ggagatgtgg
     6961 tcgagcgcga cgacgctgcc gtagtggtgg gtgacgtcct cgatcacgac cacgggcacg
     7021 accccggggc cggcggcccc ggtgccggcg gcggtgccgc tcggagtggg ctgcggcgtt
     7081 gccacggcta ctccagatcg gaggccggtg tggaaggagg tggggatggc ggaatggccg
     7141 agaccggggg cgaggcctgc gggcggccgg gcggggacgc gggcggaccg accaggttct
     7201 tctgcagcca gtcgggccac accgcggcgt cgctgatctt gacatagccg acgccgcgga
     7261 cgccggtctt gatccgctcg acgtagtgga ggacgagctc tcgcggcacc tggatcttca
     7321 cgcggaacat cagcttctct cgctcgctcc gggtctcgac ctccttgggc gtgaactgcg
     7381 cctccggcga cacgaagctc acgtatccgg gggcgacccg gttgggctcg tagtcgacgg
     7441 tgaa
//
LOCUS       DASVOR010000605         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_122527,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000605 DASVOR010000000
VERSION     DASVOR010000605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122527"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..>2137)
                     /locus_tag="VF516_11995"
     CDS             complement(387..>2137)
                     /locus_tag="VF516_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108445.1"
                     /translation="AAFRRALEADHGHRDAFLRLRILLEEDANHDELAALLAMRLEVE
                     TDPAAQIELHRALAELHRNFLSDRDTAKRHYRAILDADPNDLRAHAAVADIAWEQGNW
                     QEAADALMARAKLERVPEVLKTLCFRLGLIYADRLVDVPMALKAFQRALTYQPDDENT
                     LIRLADLATQVGEWKLALGACERLVKNESDPDRRATHLHRVARIFKHGFGDNKRAERA
                     LNLALDGAPTNDEALAQLVQFYRDANDLISVRVHLNRVAGAMRARVAADPQDGVAYRV
                     ISRAMSARDAAHVPGSLPIARAAAELAELLGTSGDPEQRLLAAQRQPDLSLLTRSEAD
                     DVLFPRGVPSELRQIFQLLGDRIAKHVGVDLRPYGVTRGDRLRGRDNPVAAVAQTVAS
                     GLGFGEIDVYISNRQPWVMIAEPTSPVSLVIGGGIASAGGDTIRFAAGDALKLAQAQL
                     AIAARLPVDDLGVLVVALLRLFQADFPIEDIDADAVAAQTQKLRRLIPTGLMNELKPF
                     ALAIDPQRFRHTDLARDLKITGLRAGLVASGSLLAGLRILSAQLGGELPAFLADPVAQ
                     GLVAFALGEDHATVAR"
BASE COUNT          291 a          705 c          844 g          297 t
ORIGIN      
        1 gccatcgagg tcggtgaggt cgggagcctg gaacttgtag gtcagcgcgt cgccgtcggg
       61 atcggtggcg tggaggttga ccatcagctc cgtgccgacc tgtgccacct gatcggtgag
      121 tccggacagc accggcggcg accccggccc gcaggctact gcggtagcca ggcaagtgac
      181 gcatatcagg gtccagcgac ccccgtagcc ggtgagtggc tctaacatgg cgacgttcct
      241 ccgagcggat ccgatggctg tcggatgcca aggtctcgag caaggagcgc accaggcagt
      301 ggggcggccc gccgcgtcgg cctctgcgca actcgtgcag cgctccccgg tgatgccgaa
      361 cccgcggctg gaacacccgc cccaggctac cgcgcgacgg tggcgtggtc ctcgccgagc
      421 gcgaacgcga ccaagccctg ggcgaccgga tcggcgagga acgccggcag ctcgccgccg
      481 agctgggccg acaggatccg caggccggcg agcagcgagc cggaggcgac caggccggcg
      541 cgcaggccgg tgatcttgag atcgcgcgcc agatcggtgt ggcggaaccg ctggggatcg
      601 atcgcgagcg cgaacggctt gagctcgttc atcaggccgg tcgggatcag ccggcgcagc
      661 ttctgggtct gggcggcgac cgcgtcggcg tcgatgtcct cgatcgggaa gtcggcctgg
      721 aacagccgga gcagcgcgac gaccaggacg ccgagatcgt cgaccggcag ccgggcggcg
      781 atcgcgagct gggcctgggc gagcttgagc gcgtcgcccg ccgcgaaccg gatcgtgtcg
      841 ccgccggcgc tggcgatgcc gccgccgatc accagcgaca ccgggctggt cggctcggcg
      901 atcatgaccc acggctggcg gttcgagatg tagacgtcga tctcgccgaa gcccaggccg
      961 ctggcgaccg tctgggcgac cgccgcgacc gggttgtcgc ggccgcgcag ccgatcgccg
     1021 cgggtgacgc cgtacggccg gaggtcgacg ccgacgtgct tggcgatccg gtcgccgagc
     1081 agctggaaga tctggcgcag ctcgctcggc acgccgcgcg ggaacagcac gtcgtcggcc
     1141 tccgaccggg tcagcagcga cagatcgggc tggcgctggg cggcgagcag gcgctgctcg
     1201 gggtcgccgc tggtgccgag cagctcggcg agctcggcgg ccgcgcgtgc gatcggcagc
     1261 gagccgggga cgtgggcggc gtcgcgcgcc gacatcgcgc gcgagatcac gcggtaggcg
     1321 acgccgtcct gcggatcggc ggcgacccgg gcgcgcatcg cgccggcgac ccggttcagg
     1381 tggacgcgca ccgagatcag gtcgttggcg tcgcggtaga actggacgag ctgggcgagc
     1441 gcctcgtcgt tggtcggtgc gccgtcgagc gcgaggttga gcgcgcgctc ggcgcgcttg
     1501 ttgtcgccga acccgtgctt gaagatcctg gcgacccggt ggaggtgggt ggcgcggcgg
     1561 tcgggatcgc tctcgttctt gaccagccgc tcgcaggcgc cgagcgcgag cttccactcg
     1621 ccgacctggg tcgcgagatc ggcgagccgg atcagggtgt tctcgtcgtc gggctggtag
     1681 gtcagcgcgc gctggaacgc cttgagcgcc atcggcacgt cgaccagccg gtcggcgtag
     1741 atcaggccga gccggaagca cagggtcttg agcacctcgg gcacgcgctc gagcttggcg
     1801 cgggccatca gcgcgtcggc cgcctcctgc cagttgccct gctcccacgc gatgtcggcg
     1861 accgcggcgt gggcgcgcag gtcgttgggg tcggcgtcga ggatcgcacg gtagtggcgc
     1921 ttggcggtgt cgcgatcgga caggaagttg cggtggagct cggcgagcgc gcggtgcagc
     1981 tcgatctggg cggccggatc ggtctcgacc tcgagccgca tcgcgagcag ggcggcgagc
     2041 tcgtcgtggt tggcgtcctc ctcgagcagg atccgcaggc ggaggaacgc gtcgcggtgg
     2101 ccgtggtcgg cctcgagcgc gcggcggaac gcggcca
//
LOCUS       DASVOR010000606         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_356456,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000606 DASVOR010000000
VERSION     DASVOR010000606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356456"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="VF516_12000"
     CDS             <1..1097
                     /locus_tag="VF516_12000"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8108446.1"
                     /translation="RDLAAARAAAPALPERRERDARVARDPRVVDLDILRITAHTPAP
                     GREPELTSVASADLFQQAGAAVRAGRRREALGLYRQLVAEFPESQYAPVALFDIAALH
                     DGEADLTATITALQELVERYPAARESVDGHLYMAALQADHGQWSEAAATLDAALARPY
                     LTFADRVEALARRGYVELEQRRYDAAEASLSAAVAEWRKAPRIDDPYYIAMAHYYRGE
                     LMHRRFAECAVRTGDDEMVADLDAKRALAVQAYDRWREALRFHQAYWATAAGYQMSQI
                     FVELWEAHVQAPYPHRIDVATRPTYVADVHTRVRPDLEKALEGHRMNVELAKAYGVDT
                     AWSRGSERQAAAIMELLARDAPGSYVAPAD"
     gene            1198..1273
                     /locus_tag="VF516_12005"
     tRNA            1198..1273
                     /locus_tag="VF516_12005"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1231..1233,aa:Thr,seq:tgt)
     gene            1300..1382
                     /locus_tag="VF516_12010"
     tRNA            1300..1382
                     /locus_tag="VF516_12010"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1334..1336,aa:Tyr,seq:gta)
     gene            1505..1580
                     /locus_tag="VF516_12015"
     tRNA            1505..1580
                     /locus_tag="VF516_12015"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1538..1540,aa:Gly,seq:tcc)
     gene            1625..1696
                     /locus_tag="VF516_12020"
     tRNA            1625..1696
                     /locus_tag="VF516_12020"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1657..1659,aa:Thr,seq:ggt)
BASE COUNT          307 a          634 c          584 g          253 t
ORIGIN      
        1 cgcgcgatct cgcagcggct cgcgcggcgg cgcctgcgct gccggagcgc cgcgagcgcg
       61 acgcgcgggt cgccagggat cctcgtgtgg tggatctcga catcctccgg atcacggcgc
      121 acacccccgc tccgggacgg gaacccgagc tgacctcggt cgcctcggcc gatctgttcc
      181 agcaggccgg cgcggcggtc cgggccggcc ggcggcgcga ggcgctcggg ctgtaccgcc
      241 agctggtcgc cgagttcccc gagtcgcagt acgccccggt cgcgctgttc gacatcgcgg
      301 cgctccacga tggcgaggcc gacctgacgg cgacgatcac ggcgctccag gagctggtgg
      361 agcgctaccc ggccgcccgc gagtcggtcg acggccacct ctatatggcc gcgctccagg
      421 ccgaccacgg gcaatggtcg gaggccgcgg cgaccctgga tgcggcgctg gcgcgcccct
      481 acctcacgtt cgccgatcga gtcgaggcgc tcgcgcggcg cggctacgtc gagctcgagc
      541 agcgccgcta cgacgccgcg gaggcctcgc tgtcggccgc ggtggccgag tggcgaaaag
      601 ccccgcggat cgacgacccg tactacatcg cgatggccca ctactaccgc ggcgagctca
      661 tgcatcggcg gttcgccgag tgcgccgtgc gcaccggcga tgacgagatg gtcgccgacc
      721 tcgacgccaa gcgcgcgctc gccgtccagg cctacgatcg ctggcgcgag gccctgcgct
      781 ttcaccaggc gtactgggcg accgcggccg gctaccagat gtcgcagatc ttcgtcgagc
      841 tctgggaagc gcacgtccag gcgccgtacc cccaccggat cgacgtcgcc acccgaccta
      901 cctatgtcgc ggatgtccac acccgggtcc ggccggatct cgagaaggcg ctggagggcc
      961 accggatgaa cgtcgagctc gccaaggcgt acggcgtcga caccgcctgg agccggggca
     1021 gcgagcggca agccgcggca atcatggagc tgctggcgcg cgatgccccg gggagctatg
     1081 ttgcgccggc agactgatcg gccaagatcc ccagcaaccg atcgcgacgg cacttgacga
     1141 gcctgggagc gtcgtctata cgtgctggcc tccgaccggc cttacccgag caatcctgct
     1201 ggcgtagctc aaccggtaga gcacctgact tgtaatcagg aggtcgcggg ttcgagtccc
     1261 accgccagct ccaagaaatc ctagcttcgc caaatagatg gagggatgcc cgagcggcca
     1321 aagggagcag actgtaaatc tgccggctca tgccttcggt ggttcgaatc cacctccctc
     1381 cacgcttcag gccacgatcc aaggccacga cccgaacgtc ggacccgaac gtcgatcgaa
     1441 cccagggaga cccaagacca tacaggcagc gcagcagcag cggccggagg cgacctagat
     1501 ccacgcgggc gtagctcagc tggtagagct ccagccttcc aagctggttg tcgagggttc
     1561 gagtcccttc gcccgctcca gacccgtcac ccgaggcatc cccccacagc aaccaccatc
     1621 ttccgccctc gtagcacagt ggcagtgcac ttccttggta aggaaggggt cgcgagttca
     1681 attctcgccg agggctcggc tacgtaaccc agggagacag ccgtgtcgaa agaaaagttc
     1741 gtccgcaaca agccgcacgt caacatcgga acgatcgg
//
LOCUS       DASVOR010000607         3022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_561258,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000607 DASVOR010000000
VERSION     DASVOR010000607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3022
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561258"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..502
                     /locus_tag="VF516_12025"
     CDS             41..502
                     /locus_tag="VF516_12025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108447.1"
                     /translation="MGEASGTRCDFQDKKSMETDKSMTRPVLRRPDDDNDGLVALPPV
                     PLVRQRLRSSVKEFAISQPGRRAAALAAVWIAATGCEADLGHYDPEEALRTYRLIESE
                     LRAELRISLGRAITNEPHPATRNTMISMLEHLEELEAAAVAPRPARRRRRR"
     gene            complement(637..2190)
                     /locus_tag="VF516_12030"
     CDS             complement(637..2190)
                     /locus_tag="VF516_12030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8108448.1"
                     /translation="MVFVSRYKSPPIPEVSLVQYFRDAVASRAGEVAVIDGPTGRSYT
                     FGQLLDLSASIAHGLIARGIQPGDRVAFVCPNLPEVALAYHGAITAGAVAMMVNPLST
                     ADELAKYFKLGSPRLAIVAAPLVGAIQAVAPGLPVIALGDAPGSEPLTALLGGPTTPP
                     AVAIAPDAMAVMPYSSGTTGVPKAVMLTHRNIIAQCLAIESVTDTDLIMPGSSVLAVL
                     PFFHIYGIMGFLTYGLMRGARLIVIPRFDLEQYIQLARRYEVPVLHVVPPILLALAKY
                     PGELQLPQLRAALVSAAPLSAELAAEFTRRTGAMVYQAYGMTELTGASHLGSLDPAHN
                     KPGSGGGLIGSAEARVVSPETGADVAPGEHGEIWVRGPFVMKGYYQDPAATAHTIDRD
                     GWLHTGDIGYVDGDGDVWIVDRVKELIKYKGMQVAPAELEAVLLQHPAVADCAVYPQP
                     DAEAGEIPKAVVVLKPGASATTDELLQFVSARVSPHKRVRVLRFVDAIPKSASGKILR
                     RVLVAQDRA"
     gene            complement(2230..>3022)
                     /locus_tag="VF516_12035"
     CDS             complement(2230..>3022)
                     /locus_tag="VF516_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108449.1"
                     /translation="AGLAGMPGVARADDDLDETPSAGSVSRGAETGASQPPASSVHSA
                     ETGGFLPTALSATSEGRHGLASVMTGWDQARRGNIYDTSAEAQLVGPVSLSAGASYDG
                     PGTIASPHVELRLDALRQARHGLDLAVAAGYVDVGFNTVPAAVFKIAAGRRVGAGYLL
                     GNVVYGQGLQAGERHGELRLAALHPITRSAQIGVDTRFQIDLERDHDEPAGETDWESR
                     TGLVASYAWNRLVVMGSAGVSALRLRAGGSTAVGPVVMAGFGTVF"
BASE COUNT          434 a         1086 c         1094 g          408 t
ORIGIN      
        1 gtgatcaggc aaggtcgcgg tccatcgcag cgcagctgtc ttgggggaag ccagcgggac
       61 ccgctgcgat tttcaggaca aaaaatccat ggaaaccgac aagtcaatga cacgccctgt
      121 cctacgtcgg cccgacgacg acaatgatgg tctggttgcc ctgccgccgg tacctctggt
      181 ccgtcagcgg cttcgcagct cggtcaagga gttcgcgatc tcgcagccgg gtcggcgtgc
      241 ggccgcactc gctgccgtct ggattgcggc gaccggatgc gaggcggacc tcgggcacta
      301 tgatcccgag gaagcgctgc gcacgtaccg cctgatcgag tccgagctcc gcgccgagct
      361 gcgcatcagc ctcggtcgcg cgatcaccaa cgagccgcac ccggcgacgc gcaacacgat
      421 gatcagcatg ctcgagcacc tggaggagct cgaagcagct gccgtcgccc cgcgccccgc
      481 gcgccgccgc cggcgccgtt gatcaccagg cagtagcacc atgccgtggc cggcccgtcg
      541 agccacgctg tacccgacga gcccggcaca accgggctcg tctggttttc ggaccgggat
      601 cggctggcct tcggcggccc ggccgcgccc ggtcgctcag gcgcggtcct gcgcgacgag
      661 cacgcggcgc aggatcttgc cggacgccga tttcgggatc gcgtcgacga agcgcagcac
      721 gcgcacccgc ttgtgcggtg acacgcgcgc ggacacgaac tgcaacagct cgtcggtggt
      781 cgcgctggcg ccgggcttga gcacgaccac ggccttcggg atctcgccgg cctcggcgtc
      841 gggctgcggg tagaccgcgc aatcggcgac cgcggggtgc tgcagcagca ccgcctcgag
      901 ctcggccggc gcgacctgca tgcccttgta cttgatcagc tccttcacgc gatcgacgat
      961 ccagacgtcg ccgtcaccgt cgacgtagcc gatgtcgccg gtgtgcagcc agccgtcgcg
     1021 atcgatcgtg tgcgcggtcg ccgcggggtc ctggtagtag cccttcatca cgaacgggcc
     1081 gcggacccag atctcgccgt gctcgccggg cgcgacgtcg gcgccggtct cgggcgacac
     1141 cacgcgggcc tcggcgctgc cgatcaggcc gccgcccgag ccgggcttgt tgtgcgccgg
     1201 atcgagcgag ccgaggtggc tcgcccccgt cagctcggtc atcccgtagg cctggtagac
     1261 catcgcgccg gtgcgccggg tgaactcggc ggcgagctcg gcggacagcg gcgcggcgct
     1321 gaccagcgcg gcccggagct gcggcagctg cagctcgccg gggtacttcg cgagcgcgag
     1381 caggatcggc ggcacgacgt ggagcaccgg cacctcgtag cgccgcgcga gctggatgta
     1441 ctgctcgagg tcgaaccgcg ggatcacgat cagccgggcg ccgcgcatca ggccgtaggt
     1501 caggaacccc atgatgccgt agatgtggaa gaacggcagc acggcgagca ccgacgagcc
     1561 cggcatgatc aggtcggtgt cggtcaccga ctcgatcgcg aggcactgcg cgatgatgtt
     1621 gcggtgggtc agcatcacgg ccttggggac ccccgtcgtg ccgctcgagt acggcatcac
     1681 cgccatcgcg tcgggcgcga tcgcgacggc gggcggcgtg gtgggcccgc cgagcagcgc
     1741 ggtcagcggc tcgctgcccg gggcgtcgcc cagcgcgatc accggcagcc cgggggcgac
     1801 ggcctggatc gcgccgacca ggggagcggc gacgatcgcg agccggggcg acccgagctt
     1861 gaagtacttc gcgagctcgt cggcggtcga cagcgggttg accatcatcg cgaccgcgcc
     1921 ggccgtgatc gcaccgtggt aggcgagcgc gacctcgggg aggttcgggc agacgaacgc
     1981 gacgcgatcg ccgggctgga tcccgcgggc gatcagtccg tgggcgatgc tcgccgacag
     2041 atcgagcagc tgtccgaacg tgtagctgcg gccggtgggg ccgtcgatca cggcgacctc
     2101 gccggcgcgc gaggcgaccg cgtcgcggaa gtactgcacc agcgagacct cggggatcgg
     2161 tggggacttg tagcggctca cgaagaccat ggggcctcag ggtgcgacgg ccgtgccggc
     2221 ccggtcaatt cagaacacgg tcccgaaccc cgccatgacg accggtccga ccgccgtgga
     2281 gccgccggcg cgcaagcgca gcgccgagac ccccgcgctg cccatgacca ccagccggtt
     2341 ccaggcgtag ctcgcgacca ggccggtccg cgactcccag tcggtctccc cggcaggctc
     2401 gtcgtggtcg cgctcgaggt cgatctggaa ccgcgtgtcg accccgatct gggcggaccg
     2461 cgtgatcggg tggagcgccg ccagccgcag ctcgccatgg cgctcgccgg cttgcagccc
     2521 ctggccgtag accacgttgc cgagcaggta gccggcgccg acgcgccgcc ccgcggcgat
     2581 cttgaacacg gcggccggca ccgtgttgaa cccgacatcg acgtaacccg cggcgactgc
     2641 gaggtccagc ccgtgcctcg cctggcgcag cgcgtcgagc cgcagctcga cgtgcggcga
     2701 ggcgatcgtc ccgggtccgt cgtagctggc gccggcgctc agcgacaccg gcccgaccag
     2761 ctgcgcctcg gccgaggtgt cgtagatgtt gccccggcgg gcctgatccc acccggtcat
     2821 gaccgaggcg agcccgtgcc ggccctcgct cgtggcggac agcgccgtgg gcaggaaccc
     2881 gccggtctcc gcgctgtgca ccgacgaggc cggcggctgg gacgctccgg tctccgcgcc
     2941 gcgggacacc gagcctgccg acggcgtctc gtcgaggtcg tcgtcggcgc gcgccacgcc
     3001 gggcatcccc gcgaggccgg cc
//
LOCUS       DASVOR010000608         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443916,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000608 DASVOR010000000
VERSION     DASVOR010000608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443916"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..2039)
                     /locus_tag="VF516_12040"
     CDS             complement(342..2039)
                     /locus_tag="VF516_12040"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX8108450.1"
                     /translation="MKMEPATIAALLRELAVYYELDGDRHRAFAFDRAARAVDGANGL
                     ARLIDEGRLEELPGVGPSIARVIAELARTGTTGVLERLRERWPPVVIELAQLPKVGVP
                     KARKIYQALAPANLDAVAALCQAGALCELPGFGKISERKILLAIEDRRSQGARMLLVD
                     AESHAASLAQHLRGDPAVTAVEICGPVRRACEVIDHLAYAVASEHRDAVIERLAGFAL
                     ITSIDRGASADPHGPVIGYLAGGLRAELHVAPPAHFGWAQIVATGSAEHVAQLRDRAA
                     ARGMRLDVLEPTDEPLVYRALGLPWIPPELRDGSDEIAAALAGDDFRDLVTLDDLTTA
                     FHCHTTYSDGKATIAEMARAAAERGFDAITITDHSAAATYAGGLDVAQLRAQLAEIDA
                     MAAPDAQILRGTEADILADGSIDVPPELIGALDVVIASVHQRYKLDRDATTARLIAAM
                     RQPFFKIWGHALGRMVLRRDPIEVRLDDVLDAVAESRAAIEINGDPHRLDLPPALARE
                     AVRRGIKFVLSCDAHSPRAIDATRFAVAMARRARIRRRDVLNALPPDELVAVIRPTG"
BASE COUNT          264 a          758 c          814 g          289 t
ORIGIN      
        1 gagcgggtgg tcctcggtgc gcaccgccag ccggcggtcc cggggcgaca ggctcggccc
       61 cctggggacc gaccacgata gcagcacgcc gtcgagctcc agccggaagt cgtaatgcat
      121 gcggcgagcg gcatgctgct ggatgacaaa gcgctggccg ggtcgtgtga cggcggcctc
      181 gccccgcggc tccgccgtct tcgtgaagtc tcgcttcgat cggtacttcg cgagcttggc
      241 agacgctgag gcggccatga cacggggtga tgcatcccgg gtgccagtgg ccccggtgcg
      301 ccgctgcggg acgcgctcag ctcgacggcg ggtcgatgcc gtcatccggt gggccggatc
      361 accgcgacca gctcgtcggg cggcagcgcg ttcaggacgt cgcggcggcg gatccgggcg
      421 cgccgcgcca tcgcgaccgc gaaccgcgtc gcgtcgatcg cccgcggcga gtgggcgtca
      481 cacgacagca cgaacttgat gccgcgccgc accgcctcgc gggccagcgc gggcgggagg
      541 tcgagccggt gcgggtcgcc gttgatctcg atcgcggcgc gcgactcggc gaccgcgtcg
      601 agcacgtcgt cgagccggac ctcgatcgga tcgcggcgca gcaccatccg gccgagcgcg
      661 tggccccaga tcttgaagaa cggctggcgc atcgccgcga tcaggcgcgc ggtcgtcgcg
      721 tcgcggtcga gcttgtagcg ctggtggacg ctggcgatca ccacgtcgag cgcgccgatc
      781 agctcgggcg gcacgtcgat cgagccgtcg gcgaggatgt cggcctcggt gccgcgcagg
      841 atctgcgcgt cgggggcggc catcgcgtcg atctcggcga gctgggcgcg gagctgggcg
      901 acgtcgagcc cgcccgcata ggtcgcggcc gcggagtggt cggtgatcgt gatcgcgtcg
      961 aacccgcgct cggcggcggc gcgcgccatc tcggcgatcg tcgccttgcc gtcgctgtac
     1021 gtggtgtggc agtggaacgc ggtggtgagg tcgtcgagcg tgaccaggtc gcggaagtca
     1081 tcgccggcga gcgccgcggc gatctcgtcg ctgccgtcgc gcagctcggg cgggatccac
     1141 ggcaggccga gcgcgcggta gaccagcggc tcgtcggtgg gctccaggac gtcgagccgc
     1201 atgccgcgcg cggcggcgcg atcgcggagc tgcgcgacgt gctcggccga gccggtggcg
     1261 acgatctggg cccagccgaa gtgggccggc ggcgcgacgt ggagctcggc gcgcaggccg
     1321 ccggcgaggt agccgatcac cggcccgtgc gggtcggcgg acgcgccgcg gtcgatcgag
     1381 gtgatcagcg cgaacccggc gagccgttcg atcacggcgt cgcggtgctc cgaggccacc
     1441 gcgtaggcca ggtggtcgat gacctcgcac gcccgccgca ccgggccgca gatctcgacc
     1501 gcggtcaccg ccggatcgcc gcgcaggtgc tgggcgagcg acgccgcgtg gctctcggcg
     1561 tcgaccagca gcatgcgcgc gccctggctc cggcggtcct cgatcgccag caggatcttg
     1621 cgctcgctga tcttgccgaa ccccggcagc tcgcacagcg cgccggcctg gcacagcgcg
     1681 gcgaccgcgt cgaggttcgc cggcgcgagc gcctggtaga tcttgcgcgc cttgggcacg
     1741 ccgaccttgg gcagctgcgc cagctcgatc acgaccggcg gccagcgctc gcgcagccgc
     1801 tccagcacgc cggtcgtgcc ggtccgcgcc agctcggcga tgacccgcgc gatcgacggc
     1861 ccgacgccgg gcagctcctc gagccggccc tcgtcgatca gccgggccag cccgttggcc
     1921 ccgtcgaccg ccctggccgc gcgatcgaac gcgaacgcgc gatggcgatc gccgtcgagc
     1981 tcgtagtaga cggcgagctc ccgcagcagc gcagcgatcg tcgccggttc catcttcatg
     2041 ggtgtaacgc cgccgggtat ctcgcgtcga gcgctcggca cgattcgcgc gtcatctcga
     2101 ggccctcacc gtggcggcgt gggtg
//
LOCUS       DASVOR010000609         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_122573,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000609 DASVOR010000000
VERSION     DASVOR010000609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122573"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VF516_12045"
     CDS             <1..184
                     /locus_tag="VF516_12045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108451.1"
                     /translation="EAAETVRDIGLAPWSAAATADRQAWVADLVERGELGGDPGAGAG
                     DDWRVHADQILRSRGG"
     gene            187..462
                     /locus_tag="VF516_12050"
     CDS             187..462
                     /locus_tag="VF516_12050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108452.1"
                     /translation="MRDALDPRLVAERLAELRRSFVAETVDEARERLAREQPRSRLSF
                     AERAHRSLEELRALCELTRVLHAVPRPPSQPISGRPPGVASDGSEKR"
     gene            590..745
                     /locus_tag="VF516_12055"
     CDS             590..745
                     /locus_tag="VF516_12055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108453.1"
                     /translation="MRAVGWAPTGQASGKHHRVWVHPDRELKLYVPDYDLIPDAVGQR
                     ILEDAEG"
     gene            742..1230
                     /locus_tag="VF516_12060"
     CDS             742..1230
                     /locus_tag="VF516_12060"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEX8108454.1"
                     /translation="MTMLIGRIEKHGRWWVALCEIVGAFTQGRSRREAMDNLAEVVEL
                     RVDRPRFKATVTELEPIGRGVLTVLIEPSEPAWLAAAVLRYQRARRGLSLADVARSLG
                     AASRNAYASYEQGQREPTLGKFRELLRAVAPEMALVLEPRASAATKRVPTRTRRAGKA
                     AA"
     gene            complement(1341..1889)
                     /locus_tag="VF516_12065"
     CDS             complement(1341..1889)
                     /locus_tag="VF516_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108455.1"
                     /translation="MQYYAMVWRSWLVLLVACGGGDGSGNGDVARFIGVYATTSHTRA
                     EMPGGSVKCTDPGQPVAAATPYFRLAVDSFFMDPDVLSVSNCTDAAATMCTDTLVTLR
                     AGGPGLQDESANSQTGGGVVCQLYFTHSDATLNGATVQIETLDKFDAPSIASSDCTLQ
                     RAQALASSPDCRDVERWTGTRQ"
     gene            complement(1961..>2354)
                     /locus_tag="VF516_12070"
     CDS             complement(1961..>2354)
                     /locus_tag="VF516_12070"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HEX8108456.1"
                     /translation="PWDGGWLRALGAAIAAAGILGEAAADAQLARWKRDPAHRSQVCD
                     AGLWAWSRHPNYFFEWCVWLGHAVYGLAFVPWGLVALAGQAVILASILKVTGIPATEA
                     QALRSKGDAYRAYQARVPAFLPRPPRRA"
BASE COUNT          365 a          777 c          879 g          333 t
ORIGIN      
        1 cgaggccgcc gagaccgtcc gcgacatcgg cctcgccccg tggtcggccg ccgcgaccgc
       61 cgatcgccag gcctgggtcg ccgacctcgt cgagcgcggc gagctcggcg gcgatcccgg
      121 cgcaggggcc ggcgacgact ggcgcgtcca cgccgaccag atcctgcgca gccggggcgg
      181 ctgagcgtgc gtgacgcgct ggacccccgg cttgttgcgg agcggctggc cgagctgcgg
      241 cgcagcttcg tcgccgagac cgtggacgag gcacgcgagc ggctggcgcg cgagcagccg
      301 cgatcgaggc tgtccttcgc ggagcgagcg cacaggtcac tggaggagct ccgggccctg
      361 tgcgagctga cgcgggtgct ccatgccgtc ccgcggccgc cgagccagcc gatctcgggg
      421 aggccgcccg gcgtggcgag cgacggcagc gagaagcgct gaccgtctgt gaacgaaagc
      481 gcggtggaga acggtggacg accgctacgc tagctgcgcg tccacacttg ttcgtgtaca
      541 acaaaggttg tacaatgcag acgtgcggcg aaacgagctc gagaggtgat tgcgcgcagt
      601 cggttgggcg cccaccgggc aggcgagcgg caagcaccac cgcgtgtggg tccatcccga
      661 ccgcgagctc aagctgtacg ttcccgacta cgacctcatc cccgacgctg tcgggcaacg
      721 catcctggaa gatgcagaag gatgaccatg ctgatcggac gcatcgagaa acacggcagg
      781 tggtgggttg ctctatgcga gatcgtcggc gcgttcacgc agggccggtc gcgcagggaa
      841 gcgatggaca acctcgcaga agtcgtcgag ctgcgcgtcg atcgtccgcg cttcaaggcg
      901 accgtgacgg agctcgagcc gataggccgc ggcgtgctta ccgtgctcat cgagccatcc
      961 gagcctgcgt ggcttgccgc ggccgtgctg aggtaccagc gcgcgcgccg cggtctgtcg
     1021 ctggccgacg tcgccagatc cctcggggcg gcgagccgaa atgcctacgc gagctacgag
     1081 caaggccagc gcgagccgac gctgggcaag ttccgcgagc ttctgcgcgc tgtcgccccg
     1141 gagatggcac tggtcctcga gccgcgtgca tctgcggcta cgaagcgcgt tccgacgaga
     1201 acgagaagag ctggtaaggc cgctgcgtga tcgcctcgac cagtgacgag gtcatttcaa
     1261 gtccgaggca accacgccga ccggatcgtg ctccgcgaaa gcgctgatca ttgacgcggt
     1321 cggcgggccg cggacgggtg ctactgccgc gtaccggtcc agcgctcgac gtcccggcag
     1381 tcgggcgagc tggcgagtgc ctgggcgcgc tggagcgtgc aatcgctcga cgcgatgctg
     1441 ggcgcgtcga acttgtcgag cgtctcgatc tggacggtcg cgccgttcag cgtcgcatcg
     1501 gaatgcgtga agtagagctg gcacaccacg ccgccgccgg tctgcgagtt ggcgctctcg
     1561 tcctgcaggc cggggccacc ggcgcgcagc gtcacgagcg tgtcggtgca catcgtggcg
     1621 gcggcgtcgg tacagttgct gacgctcagc acatccgggt ccatgaagaa gctgtcgacg
     1681 gcaagccgga agtacggcgt ggcggcggcg accggctggc cggggtccgt gcatttcacg
     1741 ctgccacccg gcatctccgc gcgggtatgc gatgtcgtgg cgtacacgcc gatgaaccgc
     1801 gcaacatcac cattgccgct gccatcaccg ccgccgcatg cgaccaggag tacgagccag
     1861 ctgcgccaga ccatggcgta gtattgcatg gcagtctgcc atctcgcggg gcacaggtgt
     1921 caggcggtgc acctcggtgg cgtgatcgtg ggccggcgag tcaggctctg cgcggtgggc
     1981 gcggcagaaa cgcgggcacg cgggcctggt aggcgcggta ggcgtcgccc ttgctgcgca
     2041 gcgcctgggc ctcggtggcc gggatgccgg tgaccttgag gatgctggcg aggatcaccg
     2101 cctggccggc gagcgcgacc aggccccacg ggacgaacgc gaggccgtag acggcgtggc
     2161 cgagccacac gcaccactcg aagaagtagt tggggtggcg cgaccaggcc cacaggccgg
     2221 cgtcgcagac ctggctgcgg tgtgcggggt cgcgcttcca gcgggcgagc tgggcgtcgg
     2281 cggcggcctc gccgaggatg ccggcggcgg cgatcgcagc gccgagcgcg cgcagccagc
     2341 cgccgtccca tggc
//
LOCUS       DASVOR010000610        21495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_356485,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000610 DASVOR010000000
VERSION     DASVOR010000610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21495
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356485"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..3608
                     /locus_tag="VF516_12075"
     CDS             42..3608
                     /locus_tag="VF516_12075"
                     /inference="COORDINATES: protein motif:HMM:NF025581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HEX8108457.1"
                     /translation="MFNALGLPEEIWRIAGPGKNGGDRVSASTLFRYGFDAWMKDGAP
                     AWSHTARRTEHDQGGAPFARLDGREQLDLPTRAGWLHSRIYADGSGREAMTKAMAEPG
                     PVPSFDATGHLERRPDGTPATHDAADRWVGTGRTVFDNKGNPVKKYEPFFSDTIAYQD
                     EQELVEWGVTPILRYDPLGRLIRTDQPNGTFARVDFDAWTTRTWDENDTVKDSVWYVH
                     RGSPDPAQPEPTKLPARRAAWLAAHHAGTFSTAHLDTLGRAFLNVADNGPAGRYETRL
                     TLDVKGNQRRLTDPRGVLALEQDFDALGRKTRTNSADAGESRTLVDVSEKPMRTWDPR
                     GYVLRREYDELRRSRYLFVTDPQSATKLAECVIYGELCDQADASNLRGRPYQLYDGAG
                     VVTNEAFDIKGNLETISRTLAKDYRGGLDWASLENEASPPVVAQKVVPLLEAEQFRID
                     ATYDALDRVVSRTTPDGKVTRQTYNEANLIERLEVDGQAYVVDIDYNARGQRLLLEYG
                     NGTQTTYDYDDKTFRLLQQVTTRPASNDTWQDLSYEYDPAGNITQIIDQVSLGRPAHA
                     PNVSPEGDLPLRLAAAYGDRAFVYDALYQLVEARGRHHMGQQADADDAPLASFVHPND
                     PQGLEPYRETFAHDPAGNILEMSCQPLGGGGGGWTRPYEYATDSNRLLGTGVPGDPPG
                     VRSAAYVHDAGGNIIQMPHLPGMHWDHDNRLQRIETQIRNSGPANNVYFTYDLTDRRV
                     RKIYEHGGLIEEHIYLGGYRRYRQQGAGIELERHTLDVLDGEHRVALVETKTIENSIK
                     ATHPDARTRFQLEDHVMSSLMELDDDGSAFSYEEYYPYGETSFRAANTAFDGSTKRYR
                     YNGKERDEETKLYYYGARYYTAGLGRWVSTDPLLYVDGPNPYMYCRNRPISLRDSRGE
                     QSQDTVEQPWMKDRPAEVLDVRRMADYEPQPDSSRTPAQRSRSQRDKVVAPPVSVTEG
                     SCTIDLRVDASRTRGAVTPSAGCESPSEPPARRYGYATRDEAAKAALSEIWEMSQLEG
                     VEYGGSIYKSNGQWGYTKAVTDKSPLHVTITGRGVPKGASSVSAYHTHVGSKDDFINA
                     MNLDVHLRFSDADRQEADSRNMPIYLANYNGEIMRYTPKKGITPLDKLDTHNSIFVSL
                     FPELKQSAPPSSDISKLLHTMSNNYEEIRLHYLQKKFTRQSH"
     gene            3765..7043
                     /locus_tag="VF516_12080"
     CDS             3765..7043
                     /locus_tag="VF516_12080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108458.1"
                     /translation="MLGESRGVELGLHTLQTISAWDALVADAAAVLEDAQLAPQFAER
                     VMKLAAPGALTRSLSRGSSGGRPAGGCAELFEGGLDVVGAAVLVSAEPSGLADLIDRM
                     FGAWRAGYRARRFERAVEDARNGRSSPDLVDYAPATSGGALEGPLDACEAAVLDALAP
                     WKGSPDAPFASAPGLPPGPVVFATGITGLSPTGGSAGTEIKITGDFAELLPRLDAGDV
                     VIGFPIADGASAQLAPKDHAPDGSWITVTVPEDARAGCVGFIDLSMLAAFNAWVERAN
                     DALASAGDALKQRLGISGAGGLGAFQPLTEPNPPCNGVNYFIGAGTEIDELTVDGSAD
                     IHVDTGSATSVTIRWAVRNASSIQISGRGFAGEEAVASAGSRVVDLPQALSQEVVTWT
                     LAAGSPGGGTISKSVTATLLAVPSFTITALEPVYTSDEGELVRHHPAVFRLYVEDGIG
                     QMGGAGVVAGLEIAIRTGSGFSTVLGPGTITTRHPGEVIPANQVGHLFSLPMAAVNDW
                     TCVEVDVRVTWSSPSGATKASASRRWAGSVSRRSVDETIYVYTTTFQGQNPTSMLLTR
                     QILGWLEQMLPVRRLYLVERGNIEIASWTAAQHTYFNDGDGNWLAYVLGQKADWWGAA
                     VAASVSHAIPAGTEAIGGAWADLASGRGFWFKDATTPEQTAYHEYGHTLQLPHPRVPS
                     SSSGTATGVDRWQPCPPAGQIGDPVAAPAGWLAGNSVMNAPPDLTNGGAGRLDLTASL
                     PLAEAVAGTVGDIMTYFPSIAPSRAVFGRIFRALLGLQDPPLIPGEQYAHCRLGPRSR
                     EPGGAWLKVVGKRSKVLATFSASRPDAQFGAEELAWVVLDTNGDTFTSGTGVDPRCYP
                     SKSTDVWILTVPWDPLASKVLRITRPGGETFDVEPAPSSELPIDARPSENHVVVSWPA
                     DPAGEGFARIECSERADGPWRDLTGILRESDVRTPGAGPCRIEADMKGLPVSASLRIR
                     VTSSVQAARRVGETTIDWIVPPPEIVWTPPPPDEVAASSIRLAVSAFAPGDGHCLEDE
                     AIQWFIEKPPGGDGSAVLEQVATGSVAFVHAAPGNLHVLVRCQDSAGRVSEARHTLSI
                     QAAT"
     gene            7118..7543
                     /locus_tag="VF516_12085"
     CDS             7118..7543
                     /locus_tag="VF516_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108459.1"
                     /translation="MNVSEMDLAGLQHLIEANPSVDWRGIALDPPAALDALVWCELDP
                     AAMTPLLKAYQRVVRILPASEERRALRLLERGLHSAIQIANLSRDEFVRRWSELFPGE
                     DALGLAVHGAALGRRSELLLRYIDDIQRNEPHYRAARFK"
     gene            7551..20579
                     /locus_tag="VF516_12090"
     CDS             7551..20579
                     /locus_tag="VF516_12090"
                     /inference="COORDINATES: protein motif:HMM:NF036757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HEX8108460.1"
                     /translation="MSTEPETNYTTLFGSMDHAEGNEARSVLSPAAYLVDLLQLRDSV
                     IHDLTMGYHARRPDVQAIRLDQASTFTEIPFLDVANAVMSAALKQVVGGTKDIDTTVN
                     HELTSNRFPPPLPFSEQHLRLQVYAEKLGTHLEEIERLYRTAVDAHRSARLRLGLSQE
                     EYDLFSTPHDEEIAALWGVDPGELDGSDLELIKKQLGLSLKELKQLVRQDLSESELNA
                     TANNAAASFFINQGPYVVLDEQAHKLQVWDGTTATQLTNRHLDRMMRLVRLARRLDVG
                     LVDLDWLIQTACGNVLDGDGLQALAIALAIRKTSDWPIDEICSLWSVPRRHGQGDGPV
                     AADLFDRVYDNDFPKTLKALVGSLRSNATADALDDRLQATLRLSGADFAFLKKALLAR
                     GAEMPRLDGESKQAYEAFYDHVVHWFSACRRIVELSRLLSVDVREVVTLLDVLGAQWA
                     VSEHEDLAVPLPFAAEAPSATPPTMPFKLLLEPSTPPFAADQLRLGTLDVLQKLIRVK
                     EWLDSRQLSVRQLAYICLEDHVDARERLKEMSSAPIDGVQPDDEIETALDDLYQALRD
                     SMLAPGALQTGSLSANGARAVFDSLREARVLIAVDAPDRALLRVLPSNHELAEAMQRG
                     IHERLELHAGDFAALGITESDPLFSLLVSHGYVERLDETVGGELEVHHFIAPGLESYF
                     SAPDSAARFTIANFQDHAEAVFSALAQRVDAARVEPAALIAAGIDSLDVPALFEVLRS
                     RNYVEQVPEAPDAYRVAAGARDLFRNPANGASFVLPNFAAVFAILAAKVEAFRRAQDN
                     CDAEANELGERLKAFSEQQTRASIRSLCGLLGLAEDLTEVAFGWAFGTPDEEMTQTLA
                     TLTLPLFRAKSKKPPEPPEPALGDAYVASRFRRLQQLALLLRKTAMTAEEAQVYLANQ
                     RVHRRLPEALKLPEGFLPASGRIDALTTLPNGDFLIINNLTGKPLGPKYVAFSRHDYH
                     LLGNMDTLDKIPGVQLPTDPLDTIPGVQLPNDPPDTFCKRVLNSGIDAAFSDTVDGKP
                     VLHLCAGDRYVTVSETGTSAASPETSTSAAKPITDWGRVRNNIQDTARVDAAVQAKDG
                     RLFLFSGDQYFVYSDPRQLLTKQTFVNERYPRSIRGKFECENVFEPKVVSPLPAPMFS
                     GVDAAFRDTDDTYFFFSGNRFTHSTDPYNLLEIRPIWGHVRNYLFEECRVDAAFVLGG
                     VTYLTRKNQLTRYSGAYQIVDETFPISFLNVPESEPLLRVLRYFPDGLDAALAGSDGR
                     LYAFKGGNYASSAAPDTSLTIRDRWGRVRNVFIDNQRVDAALALNGVVYLFCGDQYVR
                     YSGSDYTYVDEGHPLRVRPSWNARERIGLVPDGLPLPITAVAVGRAPGGTSDDVYFFG
                     GHQYAGLDGALCEIQAHWARVRNNIERTGVVDAAMLDGAGRMYLFSGDQLYRYSSPEQ
                     AFCDESYPRRLAVAWAQAGNGYSLPDAFAGGISAALRTDEGSVFLFSGQRYARADVAQ
                     TEPPRANSQDWGLVRNEIQSKNAVSAAYVDPAGKTYVFGGDQFVCYSGTAYDFVDEGF
                     PLAIATRWPTLPVDFRSDIDAALSFKSPVDGVQRLYLFKGDRYARFSSGDLTQLDAGY
                     PKQNLDAVKAEGLWFRGLAVHEPNPGSSHQDDPIVSLEAAYADTAGGQPGIVVFYRHE
                     GGVQWRREYRNGNWQAPKRMDDLTDYQPFTRLDAAFVSADGTLHAFSDNRYASRPPGG
                     SALTMAVPIRSRWGRVWNQFADLGRVDACLNMGDGRTYLFCARQYIKYTGPLRPGAPD
                     FFAVEGYPRRIDPGWPGEGVPVALAPEFQAAGYDLCRDATGKVHFFNGARTTFSGNPG
                     PDVLLTSRWGKVENRFQDLDRVDGAYRAENGKLYLFCDTQYTRYSGPVQPDAPDFWAD
                     EGYPLRIATGWRSEGLATAMPAQWSALGSAVFRDAKRTYVFQGSSYTRSQVSTSAPVI
                     PDWANVRNLIQSDNRVDAGLVLARGAGAVTLLFRGDQYVRYSGGYDGPVDEGYPKAIA
                     HLEASDGVFPGLPVPLQSGLRALFAGTDDKLHAFGPQPPDESQPQLCVSSANPGVLRP
                     LNEQWGIVDNKLWDNELVDAALLASNGKLYLFSGDQYVQYSGSDRTHVDEGYPQKIAS
                     SYAQKIGAAALAPILNQGVDAALTIGNTTFYFAQNKYVSSEHPEVAQPLVDRWGLVDN
                     RLQAGRKLDAAFVAPNGKLYLFAEHQVSVYSGASREWVDEESPRTIAADLGGQWPPEF
                     RANLDTAVFFEGRSYLFQGARHVRISDYRLTKADKGYPLDNTSKLVDRFDFELGALPD
                     WWRIKQRFDDHASQATTLLDYASQATSLLDDVKAPMADPVSRLAQATLWPQDEILAVL
                     QIYQLDQLEPPALLDCRVVARLARCFELADRMQTLPSKLDSEVWQKMFGSNAPSLAAA
                     ADFLHGLIKAATSSKDWPGVARSLQDLVQSAKRDALVAYLVKKLDKHDANDLYEHLLT
                     DVQMDASSSTSKIVEAINSIQLFYHRALIQLESFPDDGPPDPKDPSKNQIRTNLKSWW
                     PWMKNYRIWEANRKVFLHPENYIRPELRPEKSPAFQELEDKLLQDEITKDTVQDSYRR
                     YLESFNEIAQLRIVGGYRYTYRDDSDKKRLAVAMFGATRTDPPVYYYRIGEVTPANVD
                     AKEPIDWEPWQKVGITIDSDRVQPVYAFNRLFIFWIETRPFNSTAFSSSSKESYSADS
                     DSAKMVQLTVKFSFYNFTKEWVAPQTLCLNPNDPGDPEVLPTLYKKIKFWFITIIKQI
                     SAILPAAAASGVRLTARTGALAGGSQEDVIDLAFLRGFEEWKLGRLTSGLDLVLPEEA
                     DWCWKHGDILSDLAIHFPSQIGIHPRELSAIVPWDARVEEPDSEVEGSDTKKGEWFSF
                     DAKGGTFLCRPTTAPAPTPGQILSHSFSGVSAAFTTDSGDVFVFTSEKKDEKDKVNKL
                     CYYYRDHFDLTWQGPVFVGDPAWPWGRPEGVFRKYPDKRIENVIVALDGTTYFLLGTG
                     HFRYPKGGYFATIERYATELRPGSPTLEQLVGKDVPALTWDGSVAAGRSLVRAFKLPG
                     EARAAVVTQAGGKLGMVTVGLDELRQASGSTGASPFDNWDGLDTVFQVGSPERVVFSY
                     RTQLAILDWSLKIWTPGALGDLPGGAAGLSAAFSGIDDHLYYFCGDRYAEVPRDSFAG
                     PQNFDKWVDRRWGRPSLFSWLLSSVDGALRGPDGKLYLFCKEYCLTYTGVDPITLDTL
                     ACDTGVTNPPKVPQVSQVWLSPVGERHGVEQVTAAFERNGRAYLFGKQKQKDGDAFVA
                     RYSQGSQSPFHPDSGYPLPIMGISSLSVDFYARLLTADQKYMITRLTSHTSEQLGRRL
                     FARGIPGLLSLETQRQDELPSFTPLPKPLPNPPPQAGPDELFVNTEVVSEYPGKPPPS
                     PSPPVPSSEPPPLKLDFASANGFYYREIFFHIPYLIAQTLRQGQKFDEARRWYEYVFD
                     PTNPANAQFWQYVEFVDDPGRDLKPLADQVRSYRTDPFDPHKIAELRPIAYRKAFVMS
                     YIGNLLDWGDMLFRQYTRESIGEATMLYVLAADLLGKKPEELGKRAMQQADSLMYEQI
                     ASPDYTDIALTDEILMLENGVPPPTSDMPKTIQTPNDSIFNTYFYIPENADFVEYWKR
                     VDDRLSKIRHGLDIDGIKRSLALFAPPVDVMALVKAFASGAGLAETLADYSAPVPHYR
                     FAVMLARARELTGRLIGLGGALLAALEKKDAEELSQLRNTQERSILEMQLEIKQQQLA
                     GAQESLAGLQEGLKNAKAREKHYQDLLAEGLSMYEQAQIAGMIISQVFSQGANAFSLA
                     SSIANYTPQFGSLFAITYGGQQIGAGLGELAQSFRAQAETASFGSSLAATLGGWERRA
                     QDWELQKTLATGDTLQIGRQIKAAEIQVEVARQDVQVQRRMIKNNQSVDTFMNSKFTS
                     QQLYQWMAGKLSAVYFQTYHLAHQYAKAAQRAMQFELGLPESDVQYIGAGYWDSLKKG
                     LTAGEQLQLDLDRLEKAQLEANERRMEITKYVSLAQVDPLALLHLKERGTCEFELGEA
                     LFDADFPGHYGRQIKTVSLSFPALIGPYHNFNATLTQLGHRTLLAPDKARLNYLLGKT
                     TTTALDASVLRIDWRPNQQIALSTGTNDSGLFEVNYHDERYLPFEGTGAVSTWRLEIN
                     GVDGPVHRQTLSDVILTVRYTARSGGSAFAETVKNAVGGTSERAWLLNLATDFSDEWQ
                     AFLSKPSAGMAFTVERRNLPGASEKEVTGVYLHYEMVEDPVDDLSRQAIQLESGPAGP
                     QQSKYLLKPGAFTKVELPLLEPGQVPAKATWTLMPTSASAAAKLSPRNLKTIGLVVTY
                     AGKASF"
     gene            20669..>21495
                     /locus_tag="VF516_12095"
     CDS             20669..>21495
                     /locus_tag="VF516_12095"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8108461.1"
                     /translation="MTTIAHRICPFCEACCGLELAIEDGKIKTIRGYDDDVHSAGFLC
                     PKGAALRDLHEDPDRLRAPLIKRGGTHVEVSWDEAFAEIERRLVPILAEHGKDAVAMV
                     IGNPSVHKLSLTLATARLTRAIGTTHVFSASTLDQMPKQLSAGLMFGHWLSIPVPDLA
                     RTDLLVVLGANPVVSNGSLWTVPDFRGKAKAMQARGGKLIVIDPRRTETAELADQHLF
                     IRPGGDAFLLLGIVHTLFADRRVRLGRLAEHVEGLAALEAAVAEFAPERVAGRCGIEA
                     "
BASE COUNT         3815 a         7233 c         7026 g         3421 t
ORIGIN      
        1 cctcggcgat cgccgattca aaccgcaacc gcacgcgcgt cgtgttcaac gcgctcgggt
       61 tgcccgagga gatctggcgc atcgccggtc ccggaaagaa cggcggcgac cgcgtcagtg
      121 cgagcaccct cttccggtac gggttcgacg cctggatgaa ggacggagcg ccagcctggt
      181 ctcacacggc gcgcaggaca gagcacgacc agggcggcgc acccttcgcg aggctcgatg
      241 gccgggagca gctggacctg cccacccgtg ccggctggct tcactcgcgg atctacgccg
      301 acggctccgg ccgcgaggcc atgacgaagg cgatggccga gcccgggccc gtgccgtcct
      361 tcgatgcgac tggccacctc gagcgaaggc cggacggaac tccggcgact catgacgccg
      421 cagatcgctg ggtcggaacg ggccggacgg tcttcgacaa caaaggcaac ccggtcaaga
      481 agtacgagcc cttcttctcg gacacgattg cctatcagga tgagcaggag ctcgtggaat
      541 ggggcgtgac tcccatcctt cgctacgatc cgctcggccg cctgatccgc acggatcagc
      601 cgaacggcac cttcgcgcgg gtcgacttcg acgcctggac cacgcgcacc tgggacgaga
      661 acgacacggt caaggacagc gtctggtacg tccaccgtgg cagcccggat cccgcgcagc
      721 ccgagcctac caagctacct gctcgacgag ctgcctggct ggcggcccac cacgctggca
      781 ccttcagcac ggcgcacctg gacaccctcg gtcgcgcgtt cctcaacgtc gccgacaacg
      841 gaccggctgg acgatacgag acccggctca cgctcgacgt caagggtaat cagaggcggc
      901 tgaccgaccc gcgcggggtg ctcgccctcg agcaggactt cgatgcgctc gggcggaaga
      961 cccgcaccaa cagcgccgac gcgggtgagt ctcgcacgct ggtcgacgtc tccgagaaac
     1021 cgatgcggac gtgggacccg cgcggctacg tgctccgccg cgaatacgac gagcttcggc
     1081 gttccagata cctgttcgtc acagatccgc aaagcgcgac gaagctcgcc gagtgcgtga
     1141 tctacggcga gctctgtgat caggcagatg cgagcaatct tcgagggcgg ccctatcaac
     1201 tctacgacgg cgccggtgtc gtcacgaacg aggccttcga catcaagggc aacctggaga
     1261 cgatctcccg aactctcgcc aaggactatc gcggagggct ggactgggcc tcgctcgaga
     1321 acgaggcctc gcctccggtc gtcgcacaga aggtcgtccc tcttctcgag gccgagcagt
     1381 tccggatcga tgccacgtac gacgcgctcg accgcgtcgt gtctcgcacg accccggatg
     1441 gaaaggtcac ccggcagacc tacaatgagg cgaacctcat cgagagactc gaggtcgatg
     1501 gccaggccta cgtcgtagat atcgactaca acgcccgagg ccagcggctc ctcctggagt
     1561 acggcaacgg aacgcagacc acgtacgact atgacgacaa gaccttccgg ctattgcagc
     1621 aggtcacgac gcgacctgct agcaatgaca cctggcagga tctgtcctat gagtacgacc
     1681 ccgccggcaa catcactcag atcatcgacc aggtctcgct cggtagacct gcccacgctc
     1741 cgaatgtttc gcctgaaggt gatcttccgc tgcggttagc cgccgcctac ggtgaccgcg
     1801 ccttcgtcta cgacgcgctc taccaactcg tggaggcgcg aggccgccac cacatgggcc
     1861 agcaagccga tgcggacgat gcgcccctcg cgtcattcgt acaccccaac gatccacaag
     1921 ggcttgagcc ctatcgcgag acattcgccc atgatccggc tggtaacatc ctggagatgt
     1981 cctgccagcc cctcggcggc ggaggaggtg gctggacgcg accctacgag tacgccaccg
     2041 acagcaaccg gctgctggga acgggagtcc ccggggatcc ccccggcgtg cgctctgcgg
     2101 cgtacgtcca tgatgccggg ggaaatatca tccagatgcc acacctcccc ggcatgcatt
     2161 gggaccacga caaccgtctg caacgcatag aaactcagat tcgcaactca ggacctgcga
     2221 ataatgtcta tttcacctat gatcttaccg accgacgtgt ccgaaagatc tatgagcacg
     2281 gcgggctgat cgaggagcac atctatctcg gcggatacag gcgctaccgt cagcaaggcg
     2341 cgggcatcga gctagagcgc cacaccctcg atgtgctaga cggcgaacat cgcgtcgcgc
     2401 tggtcgagac aaagacgatc gagaatagca tcaaagccac tcatccggac gcgcgcacaa
     2461 ggttccaact cgaagaccac gtgatgtcat cactgatgga gctcgacgac gacggaagcg
     2521 ctttctcgta cgaagaatac tatccctatg gcgaaacctc gttccgtgca gcgaacaccg
     2581 ccttcgatgg tagcaccaag agatatcggt acaacggcaa ggagcgcgat gaagaaacaa
     2641 agctttacta ttacggagca cgctattata cagcaggact ggggcgatgg gtatcaaccg
     2701 atcctctgct atatgtcgac ggtccgaatc catacatgta ctgccgtaac aggccgatat
     2761 ctctgcgtga ttcccgtggc gaacaatcgc aagacacagt cgagcaacca tggatgaagg
     2821 atcggcctgc cgaggtactg gatgtaagac ggatggccga ctacgagcct caacctgaca
     2881 gctccagaac gcctgctcag cgatctcggt cgcaacgcga caaagtagtg gcgcctccag
     2941 taagcgtcac agagggtagt tgtacgatcg atcttcgagt agacgcatcg cggacccgag
     3001 gagccgttac accaagtgct ggttgcgaat caccctcgga gcctccggcg cggcgctacg
     3061 gctacgctac tcgtgacgaa gccgcgaagg cagcgctctc ggaaatctgg gagatgagtc
     3121 agctcgaagg cgttgagtat ggtggctcga tatacaagag caatggccaa tggggttaca
     3181 ccaaggcagt taccgacaag agtccgctcc acgtcacgat taccggtcgc ggagtcccca
     3241 agggagccag ctccgtatcg gcatatcaca cacatgtcgg atccaaggac gactttatca
     3301 atgcgatgaa cctggatgtc cacctacgat tctcagatgc ggacaggcaa gaggccgaca
     3361 gtcgaaacat gccgatttat ctggccaact acaacggcga aatcatgcga tatacgccca
     3421 agaaaggcat aacgccactt gataagctag atacgcataa cagtatcttt gtaagcctct
     3481 tccctgagct caagcaatcc gcaccgccct cgtctgatat cagcaagcta ctacatacca
     3541 tgagcaataa ttatgaggag ataagattgc actatctgca gaagaagttc acaaggcagt
     3601 cgcactgaca tggaattatc accgcggaga gaagaaggat catgagcgct catggatatc
     3661 tagcgttgtg cggagcgaca ccgatgatgg gccagacgcc aagcagccag cacggatgcg
     3721 cgtgccccaa catagctcac gggccgctgc gctcattcgt cgaagtgctc ggtgagtcgc
     3781 gcggggtcga gctcgggctc cacacgctcc agacgatctc cgcgtgggat gcgctcgtgg
     3841 cggacgccgc cgccgtgctc gaggacgccc agcttgcgcc gcagttcgcg gagcgggtca
     3901 tgaagctcgc cgcgcccgga gctctgacgc gaagtctgtc cagaggtagc tcgggaggtc
     3961 gccccgccgg cggctgtgcg gaactgttcg agggtgggct cgacgtcgta ggcgcggccg
     4021 tcctcgtgtc ggcggagccg tcggggctgg ctgacttgat cgatcggatg ttcggggcat
     4081 ggcgtgccgg ctatcgcgcg aggcgcttcg agcgtgcggt cgaggatgcg cgcaatgggc
     4141 ggtcgagccc ggatctcgtc gattacgctc cagcgacgag cggcggagcc ctggagggac
     4201 cgctcgatgc ttgcgaggcg gcggtgctcg atgctctggc cccctggaag ggctcgcccg
     4261 atgccccgtt cgcgtcggcg ccgggactgc cgccgggccc cgtggtgttc gccaccggca
     4321 tcaccggact gtcgcccacc ggaggaagcg ccggcacgga gatcaagatc accggcgact
     4381 tcgcagagct gcttcctcga ctggacgcgg gggacgtggt gatcgggttc ccgatcgcgg
     4441 atggtgcgag tgcgcagctc gcgccgaagg accacgcccc ggacggttcc tggatcaccg
     4501 tgacggtccc ggaggacgcc cgcgccggat gcgtcgggtt catcgacctg tcgatgctgg
     4561 ctgcgttcaa tgcctgggtc gagcgggcga acgatgcgct ggccagtgct ggggacgcgc
     4621 tgaagcagcg ccttggtatc tccggagctg ggggactcgg cgcgttccag ccactcaccg
     4681 agccgaaccc gccgtgcaat ggcgtgaact acttcatcgg ggccgggacc gagatcgacg
     4741 agctcacggt cgatggctcg gcggatatcc acgtggatac gggcagcgcg accagcgtga
     4801 ccatccgatg ggcggtacgg aacgcgtcga gcatccagat cagcggccgt ggcttcgccg
     4861 gtgaggaggc ggtcgcatcg gcggggagca gggtcgtcga tctcccacag gccctgagcc
     4921 aggaggtcgt cacgtggacg ctcgcggccg gctcgcccgg cggcggcacg atcagcaagt
     4981 ccgtcaccgc gaccctcctg gccgttccgt cgttcacgat caccgcgctc gaaccggtct
     5041 acaccagcga cgaaggcgag ctggtccgtc accaccctgc ggtcttccgg ctctatgtcg
     5101 aggacgggat cggtcagatg ggcggagccg gggtcgtcgc cgggctggag atcgcgatcc
     5161 ggacgggcag cgggttttcc accgtcctcg gcccgggaac gatcacgact cgacatcccg
     5221 gagaggtcat cccggccaac caggtcggtc atctgttctc gctgccgatg gccgcggtca
     5281 acgactggac atgcgtggag gtcgacgtac gcgtcacgtg gtccagcccg agcggcgcga
     5341 cgaaggcgag cgcgagccgc cggtgggcag gcagcgtgtc gcgccggtcc gtcgacgaga
     5401 cgatctacgt ctacaccacg acgttccagg gccagaaccc gacgagcatg ctgctcacgc
     5461 ggcagatcct gggctggctg gagcagatgc tgccggtccg ccgcctgtac ctcgtggagc
     5521 gaggcaacat cgagatcgcg agctggaccg ctgcccagca cacgtacttc aacgatggag
     5581 acggcaactg gctggcgtat gtcctcggac agaaggccga ttggtggggc gcggcggtcg
     5641 ccgccagcgt cagccatgcc attcccgccg gcaccgaggc gatcggagga gcctgggccg
     5701 atctcgcgag cgggagaggc ttctggttca aggacgcgac cacgccggag caaactgcct
     5761 atcacgagta cggccatacc ttgcagctcc cgcatccccg cgtcccgagc tcgtcctccg
     5821 gtacggccac cggcgtcgat cgatggcagc cgtgtcctcc cgccggccag atcggcgatc
     5881 cggtcgcagc tccggccggc tggctggccg gcaattcggt gatgaatgcg ccacccgatc
     5941 tgacgaatgg cggagcgggc cggctcgacc tcacggcctc gctgcccctc gccgaggcgg
     6001 tcgcgggaac ggtcggcgac atcatgacgt acttcccctc catcgcgccg agccgtgccg
     6061 tgttcggacg catcttccgt gcgctcctgg gcttgcagga ccctccgctg atcccgggcg
     6121 agcaatacgc ccattgccgg ctcggccccc gcagccgcga gcccggagga gcgtggttga
     6181 aggtcgtcgg gaagcgctcg aaggttctgg cgacgttctc ggccagccgc ccggacgcgc
     6241 agtttggtgc cgaggagctc gcctgggtcg tgctcgatac caacggggac acgttcacgt
     6301 cgggcaccgg cgtcgatccc cgctgctacc cgagcaagtc cacggacgtg tggatcctga
     6361 ccgtgccctg ggaccccctc gcttcgaagg tcttgcgcat cacgcgcccc ggcggcgaga
     6421 cgttcgacgt ggagccagcg ccgagctccg agctgcccat cgacgcccgg ccttccgaga
     6481 accacgtcgt cgtgagctgg ccggcggatc cggctggtga aggatttgcg cggatcgaat
     6541 gttccgagag agcagacggg ccatggcgcg atctcacggg gatcctccgg gaatccgatg
     6601 tccggacacc cggcgcgggg ccctgccgga tcgaagccga catgaagggt ctgccggtgt
     6661 ccgcgagctt gcggattcgc gtgacctcca gcgtccaggc ggcacgacgt gtaggcgaga
     6721 ccacgatcga ctggatcgtc ccgccgccgg agatcgtctg gacgccaccg ccgcccgatg
     6781 aggtggcggc ctcgtcgatc cgactcgcgg tctccgcgtt cgcgccaggt gatggtcatt
     6841 gcctcgaaga cgaggcgatc cagtggttca tcgagaaacc gccgggtggt gacggatccg
     6901 ctgtcctgga gcaagtggcg acgggctcgg tggccttcgt tcacgcagcc ccgggtaacc
     6961 tccacgtcct cgtcagatgc caggacagcg ccggtcgggt gtccgaggca aggcacaccc
     7021 tctcgatcca agctgcgacg tgaacgagag gcgggataca cgacaaccga cctgagagat
     7081 cgcttcagtc aaggagggag acacgatgcc agtcacaatg aacgtgagcg agatggacct
     7141 ggcggggctg caacacttga tcgaagcgaa cccctcggtc gactggcgcg ggatcgcgct
     7201 ggatccgccg gcggcgctgg acgcgctggt gtggtgtgag ctggatccgg cggcgatgac
     7261 gcctctgctg aaggcgtacc agcgagtggt gcgcatcctg cccgcgtccg aggagcgccg
     7321 cgcgctgcgg ctgctcgagc gtgggctcca ctccgcgatc cagatcgcga acctgtcccg
     7381 ggacgagttc gtccgccgct ggagcgagct gttccccgga gaggacgccc tggggctcgc
     7441 cgttcacggc gccgcgctcg gccggcgcag cgagctcctc ctccggtaca tcgacgatat
     7501 ccagcgcaac gaacctcatt accgcgccgc gcggttcaag tgagcagacc atgagcaccg
     7561 aaccagagac caattacacg accctcttcg gatcgatgga tcacgccgaa ggaaacgagg
     7621 cgcgctcggt cctctcgccg gccgcgtacc tcgtggacct gctccagctg cgggacagcg
     7681 tgatccatga tctgacgatg ggctatcacg cgcgccgtcc ggacgtccag gccatccggc
     7741 tggaccaggc gagcacgttc accgagatcc cgttcctcga cgtcgccaac gccgtgatga
     7801 gcgcggcgct gaaacaggtg gtcggcggca ccaaagatat cgacaccacc gtcaaccacg
     7861 agctcaccag caaccggttc ccgccgccgc tgccattctc ggagcagcac ctgcgcctcc
     7921 aggtgtatgc cgagaagctc ggcacccacc tggaggagat cgagcggctc tatcgaaccg
     7981 cggtggatgc ccaccggtcg gcgcggctcc gcctcgggct gagccaggag gagtacgatc
     8041 tgttctccac gccgcatgac gaggagatcg ctgcgctctg gggcgtggat ccgggggagc
     8101 tggacggctc ggacctcgag ctgatcaaga agcagctggg gctctcgctg aaggagctca
     8161 agcagctcgt ccgccaggac ctgtccgagt cggagctgaa cgccacggcg aacaacgccg
     8221 cggcgtcgtt cttcatcaac cagggtccgt acgtcgtcct cgacgagcag gcgcacaagc
     8281 tgcaggtctg ggacggaacg acggcgacgc agctgacgaa ccgtcacctg gatcggatga
     8341 tgcgcctggt ccgcctcgcg cggcgcctgg acgtggggct ggtggatctg gactggctga
     8401 tccagacggc ctgcggcaac gtgctcgatg gcgacgggct tcaagcgctc gcgatcgccc
     8461 tcgcgattcg caagaccagc gactggccga tcgatgagat ctgcagcctg tggagcgtac
     8521 ccaggcgcca cggccagggg gacggcccgg tggcggcgga cctgttcgat cgcgtctacg
     8581 acaacgactt tccgaagacc ctgaaggcgc tggtcggcag cctgcgctcg aacgccaccg
     8641 cggatgcgct ggacgatcgg ctgcaggcga cgctgcgcct gtccggagcc gacttcgcct
     8701 tcctcaagaa ggcgctcctc gcgcgcggcg cggagatgcc gaggctcgat ggagagtcga
     8761 agcaggcata cgaagcgttc tacgaccacg tcgtccactg gttctccgca tgtcgccgga
     8821 tcgtcgagct gtccaggctg ctctcggtcg acgtccggga ggtggtgacg ctgctcgacg
     8881 tgctcggcgc gcagtgggcc gtctccgagc acgaggacct cgccgtcccg ctgccgttcg
     8941 cggccgaggc gccgtccgcg acgccgccca ccatgccgtt caagctgctg ctcgagccca
     9001 gcacgccgcc gttcgcggcc gaccagctgc ggctcgggac gctggacgtc ctgcagaagc
     9061 tgatccgcgt gaaggagtgg ctggactcgc gccagctctc ggtccgccag ctcgcgtaca
     9121 tctgcctcga ggatcacgtc gatgcacgcg agcgcctgaa ggagatgagc agcgcgccga
     9181 tcgacggagt ccagcccgac gacgagatcg agacggcgct ggacgatctg taccaggcgc
     9241 tccgcgactc gatgctggcg ccgggcgcgc tgcagacggg ctcgctcagc gcgaacggcg
     9301 cgcgcgcggt gttcgactcg ctccgggagg cgcgggtgct gatcgcggtc gacgcgccgg
     9361 accgcgcgct gctccgcgtc ctgccgtcga accacgagct ggcggaggcc atgcagcgcg
     9421 ggatccacga gcggctggag ctccacgcgg gtgacttcgc tgccctcggg atcaccgagt
     9481 cggatccact gttctcgctg ctggtgtccc acgggtacgt ggagcgcctc gacgagaccg
     9541 tcggcgggga gctcgaggtc caccacttca tcgcgcccgg gctggagagc tacttctcgg
     9601 ctccggacag tgcggccagg ttcacgatcg cgaacttcca ggaccacgcc gaggcggtgt
     9661 tctccgcgct ggcgcagcgc gtggatgcgg cgcgcgtgga gccggccgcg ctcatcgccg
     9721 ccgggatcga ctccctggat gtgccggcgc tgttcgaggt gctccggtct cgaaactacg
     9781 tggagcaggt ccccgaggcc ccggatgcgt accgcgtcgc cgccggtgcc agggatttgt
     9841 tccggaaccc tgcgaacggg gcgagcttcg tcctccccaa cttcgccgcg gtgttcgcga
     9901 tcctcgccgc caaggtggag gccttccggc gcgcgcagga caactgcgac gcggaggcga
     9961 acgagctcgg cgagcggctg aaggccttct ccgagcagca gacccgcgcg tcgatccgga
    10021 gcctctgcgg tcttttggga ctcgccgagg acctcaccga ggtcgccttt ggctgggcgt
    10081 tcggtacccc cgacgaggag atgacgcaga cgctcgcgac attgacgctg ccgctgttcc
    10141 gggccaagag caagaagcca cccgagccac ccgagcccgc gctgggggac gcgtacgtgg
    10201 cgtctcgctt ccgccgcctc cagcagctcg cgctcctgct ccgcaagacg gccatgaccg
    10261 cggaggaggc gcaggtctac ctcgcaaacc agcgggtcca ccggcgcttg cccgaggcgc
    10321 tgaagctgcc cgagggtttc ctgcccgcct ccgggcgcat tgacgccctc accacgctgc
    10381 cgaacggcga cttcctgatc atcaacaatc tcaccggcaa gccgctcggt ccgaagtacg
    10441 tggcgttttc gcggcatgac tatcacctgc tcggcaacat ggacacgctg gacaagatcc
    10501 ccggcgtcca gctgccgact gatccgctgg acacgatccc tggcgtccag ctgccgaatg
    10561 atccaccgga tacgttctgc aagcgcgtgc tgaacagcgg catcgacgca gcattcagcg
    10621 acaccgtgga cggcaagccc gtgctccatc tctgcgccgg cgatcggtac gtgacggtgt
    10681 ccgagaccgg caccagtgca gccagcccgg agaccagcac cagtgcagcc aagccgatca
    10741 cggactgggg cagggtccgc aacaacatcc aggacaccgc gcgcgtggac gccgcggtac
    10801 aggccaagga tgggcggctg ttcctgttca gcggcgacca gtacttcgtc tacagcgatc
    10861 cccggcagct cctcacgaag caaaccttcg tgaacgagcg atacccgcgc tcgatccgag
    10921 gaaagttcga gtgcgagaac gtcttcgagc ccaaggtcgt ctcaccgctc cctgcgccga
    10981 tgttctcggg ggtggacgct gccttccggg acacggacga cacgtacttc ttcttctcgg
    11041 ggaaccgctt cacccactcc accgatccgt acaatctcct cgagatccgc ccgatctggg
    11101 gccatgtgcg caactacctg ttcgaggaat gccgcgtcga cgcggcgttc gtcctcggcg
    11161 gtgtcaccta cctcacgcgc aagaatcagc tcacccggta cagcggggcg taccagatcg
    11221 tcgacgagac cttcccgatc tcgttcctca atgtcccgga gagcgagccg ctgctgcgcg
    11281 tcctccggta cttcccggac gggctggacg ccgcgctggc cggcagcgat ggccggctct
    11341 atgccttcaa gggcgggaac tacgcgagct cggccgcccc cgacacgtcc ctgaccatcc
    11401 gcgaccgctg gggtcgggtc cggaacgtgt tcatcgacaa ccagcgcgtg gacgctgccc
    11461 tggccctgaa cggcgtggtc tacctgttct gcggcgatca gtacgttcgc tactccggct
    11521 cggactacac gtacgtggac gagggccatc cactgcgcgt gcgccccagc tggaacgcgc
    11581 gggagcggat cggcctcgtc ccggacgggc tcccgctgcc gatcaccgcg gtggcggtgg
    11641 gccgagcgcc cgggggaacg tcggacgacg tctacttctt cggcggccat cagtacgccg
    11701 gcctcgacgg cgcactctgc gagatccagg cgcactgggc gcgcgtccgc aacaacatcg
    11761 agcgcaccgg cgtggtcgac gcggcgatgc tcgacggcgc cgggcggatg tacctgttct
    11821 cgggtgatca gctctatcgc tacagctcac cggagcaggc gttctgcgac gagtcctatc
    11881 cccggcgcct ggccgtcgcc tgggcgcagg cgggaaacgg gtattctctg cccgatgcgt
    11941 tcgccggcgg catctcggcg gcgctgcgca ccgacgaggg cagcgttttc ttgttcagcg
    12001 gccagcgcta cgcacgcgcc gacgtcgcgc agaccgagcc gccgcgcgcg aacagccagg
    12061 actggggcct ggtccgcaac gagatccagt cgaagaacgc ggtgagcgcc gcgtacgtgg
    12121 atccggcggg caagacgtac gtgttcggcg gcgatcagtt cgtctgctac tcgggcaccg
    12181 cctacgactt cgtcgacgag ggtttcccgc tggcgatcgc cacgcggtgg ccaaccctcc
    12241 cggtcgactt ccgcagcgac atcgatgcgg cgctgtcgtt caagtcgccg gtcgacggcg
    12301 tacagcggct gtacctgttc aagggagacc gctacgcgcg cttcagctcg ggcgacctca
    12361 cccagctcga tgccggttac ccgaagcaga acctggacgc ggtcaaggcc gaggggctct
    12421 ggttccgcgg cctcgccgtg catgaaccga acccgggctc gtcgcaccag gatgatccga
    12481 tcgtctcgct ggaggccgcc tacgccgaca ccgccggcgg ccagcccggg atcgtcgtct
    12541 tctaccgtca cgaaggtggg gtgcagtgga ggcgcgagta ccgcaacggg aactggcagg
    12601 cgccgaagcg catggatgat ctcaccgact atcagccgtt cacgcggctg gacgcggcct
    12661 tcgtctcggc cgacggtacc ctgcacgcgt tctccgacaa ccgatacgct tcgcgcccgc
    12721 cggggggcag cgcgctcacg atggcggtgc cgatccgcag ccgctggggg cgcgtgtgga
    12781 atcagttcgc cgatctcggc cgggtggacg cctgcctcaa catgggtgac gggcgcacct
    12841 acctgttctg cgcccggcag tacatcaagt acaccggccc gctgcgtccc ggtgcacccg
    12901 acttcttcgc ggtcgagggc taccccaggc gcatcgatcc cggctggcct ggcgagggtg
    12961 tgccggtggc gctggcgccc gaattccagg cagcgggata cgacctgtgc cgcgacgcca
    13021 cgggcaaggt tcacttcttc aacggagctc gaaccacgtt ctcgggcaac cccggtccag
    13081 acgtactgct gacgtcccgc tgggggaagg tggagaaccg gttccaggac ctcgatcgcg
    13141 tcgacggggc gtatcgcgcc gagaacggca agctctatct gttctgcgat acgcagtaca
    13201 cgcgctacag cggccccgtg cagccggatg caccggattt ctgggcggac gagggctatc
    13261 cgctgcgcat cgccaccggc tggcggtcgg aggggctggc gaccgcgatg cccgcgcaat
    13321 ggagcgcgct cgggtccgcg gtgttccgcg acgcgaagcg gacgtacgtg ttccagggct
    13381 cctcctacac ccgctcgcag gtgtcgacgt ccgcgccggt catcccggac tgggcgaacg
    13441 tgcggaacct gatccagtcg gacaaccgcg tggacgccgg cctcgtgctt gcccgcggcg
    13501 ccggcgcggt gacgctgctg ttccgcggcg atcagtacgt ccgctacagc ggtgggtacg
    13561 acggtcccgt cgacgagggg taccccaagg ccatcgcgca tctcgaggcc tcggatggtg
    13621 tgttccccgg gcttccggtt ccgctccagt ccggcctccg cgcactcttc gccgggaccg
    13681 acgacaagct ccacgccttc gggccgcagc cacccgacga gtcccagccg cagctttgcg
    13741 tctcctccgc caatcccggc gtgttgcgcc cgctgaacga gcagtggggc atcgtcgata
    13801 acaagctctg ggacaacgag ctcgtcgatg cggcgctgct ggcatcgaac gggaagctct
    13861 acctgttcag cggcgatcag tacgtccagt actcgggatc ggatcgcacg cacgtcgacg
    13921 agggctaccc gcagaagatc gcatcctcgt acgcgcagaa gatcggcgcc gctgcactcg
    13981 cgccgatcct gaaccagggc gtggatgcgg cgctgaccat cggcaacacg acattctact
    14041 tcgcccagaa caagtacgtc agctcggagc accccgaggt ggctcagccg ctggtggacc
    14101 gctggggcct cgtcgataac cggctccagg ccggccggaa gctcgacgcc gccttcgtgg
    14161 cgccgaatgg caagctctac ctgttcgccg agcaccaggt ttcggtctac tcgggcgcga
    14221 gccgagagtg ggtggacgag gagtccccca gaaccatcgc cgcggacctc ggcgggcaat
    14281 ggcctccaga gtttcgtgcc aacctggaca ccgctgtctt cttcgagggc cgtagctacc
    14341 tgttccaggg cgctcggcac gtccggatct cggactatcg cctgacaaag gcagacaagg
    14401 ggtatccgct tgataacacc agcaagctgg tggatcgctt cgacttcgag ctgggtgcgc
    14461 tcccggactg gtggcggatc aagcagcggt tcgacgatca cgcctcgcag gcgaccactc
    14521 tcctcgatta cgcctcgcag gcgacctctc tcctcgacga cgtcaaggcc ccgatggcgg
    14581 acccggtgtc caggctggcg caagccacgc tatggccgca ggacgagatc ctcgcggtgc
    14641 tgcagatcta ccagctcgac caactcgaac cgcccgcgct cctggactgc cgggtggtcg
    14701 cccggctggc gcgctgcttc gagctggcgg accggatgca gaccctcccc tccaagctcg
    14761 actcggaggt ctggcagaag atgtttggct cgaatgcgcc ttcgctcgca gcagcagccg
    14821 acttcctcca tgggctgatc aaggcggcga ccagctcgaa ggactggccg ggtgtggcgc
    14881 ggtcactcca ggatctggtg cagtctgcca agcgagatgc gctggtggcg tacctcgtga
    14941 agaagctcga caagcacgac gccaacgacc tctacgagca cctgctcacc gacgtccaga
    15001 tggacgcgtc ctccagtacc tcgaagatcg tggaggcgat caacagcatc cagctgttct
    15061 atcatcgcgc gctgatccag ctcgagagct ttccagacga cggccccccg gatcccaagg
    15121 atcccagcaa gaaccagatc aggacgaacc tcaagtcgtg gtggccctgg atgaagaact
    15181 accggatctg ggaggcgaac cggaaggtct tcctccaccc ggagaactac atccgcccgg
    15241 agctgcgacc ggagaagagc cccgccttcc aggagctgga ggacaagctg ctgcaggacg
    15301 agatcaccaa ggacaccgtc caggacagct accggcgata cctcgagtcg ttcaacgaga
    15361 tcgcgcagct ccgcatcgtc ggcggatacc gctacacgta cagggacgac tccgacaaga
    15421 aacggttggc ggtcgccatg ttcggcgcca cccggacgga tccaccggtc tactattacc
    15481 ggatcggcga ggtcactccg gcgaacgtcg acgccaagga gccgatcgac tgggagccgt
    15541 ggcagaaggt cggcatcacc atcgactccg accgtgtgca gccggtgtac gcgttcaacc
    15601 ggctgttcat cttctggatc gagacccgac cgttcaactc gacggcgttc tcctccagct
    15661 ccaaggagag ctacagcgcc gattcagact cggcgaagat ggtccagctc acggtcaagt
    15721 tctccttcta caacttcacc aaggagtggg tggcaccaca gacgctctgc ctcaacccca
    15781 acgacccggg cgacccggag gtactcccca ccctctacaa gaagatcaag ttctggttca
    15841 tcacgatcat caagcagatc tctgcgatcc ttccggccgc cgccgccagc ggtgtgcggc
    15901 tcaccgcccg gaccggcgcg ctggccggtg gcagccagga agacgttatc gatctcgcct
    15961 tcctgcgtgg attcgaggag tggaagctcg ggcgcctcac ctccgggctc gacctggtgc
    16021 tgccggagga ggcggactgg tgctggaagc acggcgacat cctctcggat ctggcgatcc
    16081 acttcccttc ccagatcggg attcaccccc gcgagctgag tgccatcgtc ccatgggacg
    16141 ctcgcgtgga ggagcccgac tccgaggtgg agggatccga caccaagaag ggcgagtggt
    16201 tcagcttcga cgccaagggc ggcacgttcc tctgccgacc gacgaccgcc cctgccccga
    16261 cgccaggaca gatcctgagc cattccttct ccggggtttc ggccgccttc accacggact
    16321 ccggagatgt cttcgtcttc acctcggaga agaaagacga gaaagacaag gtcaacaagc
    16381 tctgctacta ttaccgcgac cacttcgacc tgacctggca gggcccggtg ttcgtgggag
    16441 acccggcgtg gccgtggggc cggcccgaag gcgtgttccg gaaatatccg gacaagcgga
    16501 tcgagaacgt catcgtggca ctcgatggca ccacgtattt cttgctcggc accggtcact
    16561 tcaggtatcc gaaaggcggc tacttcgcga cgatcgagcg ctacgcgacc gagctgaggc
    16621 cgggctcgcc caccctggag cagctcgtcg ggaaggatgt tccggctctg acatgggacg
    16681 gctcggtcgc cgcggggcgg tcgctggtgc gagccttcaa gctccccggg gaggctcgcg
    16741 cagcggtggt gacccaggcc ggcgggaagc tgggtatggt cacggtcggc ctcgacgagc
    16801 tgcgccaggc ctccggctcg accggcgcca gccccttcga caactgggac ggactggaca
    16861 ccgtcttcca ggtcggcagt ccggagcgtg tggtcttcag ctacaggacg cagctcgcga
    16921 tcctcgactg gtccttgaag atctggaccc cgggcgccct gggagacctt ccgggcggtg
    16981 ccgcgggcct gtccgccgca ttcagcggca tcgacgatca tctctactac ttctgcggcg
    17041 accggtacgc ggaggtcccc cgggacagct tcgccggacc tcagaacttc gacaagtggg
    17101 tcgacagacg ctggggccgg ccgtccctgt tctcctggct gctgtcgtcc gtggacggcg
    17161 cgctgcgcgg cccggacggc aagctctatc tgttctgcaa ggagtattgc ctcacctaca
    17221 ccggagtcga tccgatcacg ctggacactc tggcttgcga taccggcgtc acgaatccac
    17281 cgaaggtgcc gcaagtgtcg caggtgtggc tcagcccggt gggtgagcgg cacggggtgg
    17341 agcaggtgac cgcggcgttc gagcgcaatg gccgcgccta tctgttcgga aagcagaagc
    17401 agaaggatgg cgacgcgttc gtcgcacgtt actcacaggg ctcgcagagc ccgttccatc
    17461 cggactccgg gtatcccctg ccgatcatgg ggatctccag cttgtcggtc gacttctacg
    17521 cacggctcct gacggcggac cagaagtaca tgatcacccg gttgaccagc catacctcgg
    17581 agcagctggg gcggcggctg ttcgcgcgtg ggatcccggg gctgctgtcg ctggagaccc
    17641 agcggcagga cgagctcccg agcttcacgc cgttgcccaa gccgttgccc aacccaccgc
    17701 cgcaagcggg gccggatgag ctgttcgtca acaccgaagt tgtctcggag tacccgggca
    17761 agccgccacc ctctccttct ccccccgttc cctcgtctga gcctcctccg ctcaagctcg
    17821 acttcgccag cgccaacggc ttttactacc gcgagatctt cttccatatc ccgtacctga
    17881 tcgcccagac gctcaggcag gggcagaagt tcgacgaggc ccgccgctgg tacgagtacg
    17941 tgttcgatcc caccaacccg gccaacgccc agttctggca gtacgtcgaa ttcgtcgacg
    18001 atcccggccg cgatctcaag ccgctggccg accaggtccg ctcctatcgg acggatccgt
    18061 tcgacccgca caagatcgcg gagctacggc cgatcgcgta tcgcaaggcg ttcgtgatga
    18121 gctacatcgg caatctgctg gactggggtg acatgctgtt ccggcagtac acgcgcgaga
    18181 gtatcggcga ggccaccatg ctctacgtgc tggcagctga tctcctcggc aagaaaccgg
    18241 aggagcttgg caagcgcgcg atgcagcagg ccgactcgct catgtacgag cagattgcca
    18301 gccctgatta cacggacatc gccctcaccg acgagatcct gatgctggag aacggcgtcc
    18361 cgccgcccac ctccgacatg cccaagacga tccagactcc caatgacagc atcttcaaca
    18421 cgtacttcta catcccggag aacgccgact tcgtcgagta ctggaagcgg gtggacgacc
    18481 ggctgagcaa gatccggcac ggcctcgaca tcgacgggat caagcgctcg ctggcgctgt
    18541 tcgccccgcc ggtggacgtg atggccctgg tgaaggcgtt cgcctccggc gccgggctcg
    18601 cggagacgct ggcggactac agcgcgccgg tgccgcacta ccggttcgcc gtgatgctcg
    18661 cgcgcgcccg cgagctgacg ggccggctga tcgggctggg tggcgcgctg ctcgccgcgc
    18721 tggagaagaa ggacgccgag gagctgagcc agctccgcaa cacgcaggag cgctcgatcc
    18781 tcgagatgca gctcgagatc aagcagcagc agctcgcggg cgcccaggag tccctggccg
    18841 gcctccagga gggcctcaag aacgccaagg cgcgcgagaa gcactaccag gatctcctgg
    18901 ccgaggggct ctccatgtac gagcaggcgc agatcgccgg gatgatcatc agtcaggtgt
    18961 tctcgcaggg cgcgaacgcc ttcagcctgg cgtcctcgat cgccaactac acgccgcagt
    19021 tcggctcgct gttcgcgatc acgtacggcg gccagcagat cggcgcgggt ctcggggagc
    19081 tcgcgcagtc gttccgggcg caagccgaga cggcctcgtt cgggagctcg ctggccgcga
    19141 cgctcggcgg gtgggagcgc cgcgcccagg actgggagct ccagaagacc ctcgccaccg
    19201 gcgacacgct gcagatcggc cggcagatca aggcggcgga gatccaggtc gaggtcgccc
    19261 gccaggacgt ccaggtccag cgccggatga tcaagaacaa ccagtcggtc gacaccttca
    19321 tgaattcgaa gttcaccagc cagcagctct accagtggat ggccgggaag ctgtccgcgg
    19381 tgtacttcca gacctatcac ctggcgcatc agtacgcgaa ggccgcccag cgcgcgatgc
    19441 agttcgagct ggggcttccg gaatcggacg tccagtacat cggcgccggc tactgggaca
    19501 gcctcaagaa gggcctgacc gcgggcgagc agctccagct cgacctggat cggctggaga
    19561 aggcccagct cgaggcgaac gagcgacgga tggagatcac gaagtacgtc tcgctggcgc
    19621 aggtcgaccc gctggccctg ctccacctca aggagcgggg gacctgcgag ttcgagctcg
    19681 gcgaggcgct gttcgacgcg gacttcccgg gccactacgg ccgccagatc aagacggtgt
    19741 cgctgtcgtt cccggccctg atcggcccgt accacaactt caacgccacc ctgacccagc
    19801 tcggccaccg cacgctgctg gcgccggaca aggccaggct gaactacctg ctggggaaga
    19861 cgaccaccac ggcgctggac gccagcgtcc tccgcatcga ctggcgcccg aaccagcaga
    19921 tcgcgctctc caccggcacc aacgacagcg gcctgttcga ggtcaactac cacgacgagc
    19981 gctacctgcc gttcgagggg accggggcgg tgtcgacctg gcgcctcgag atcaacggcg
    20041 tggacggccc ggtccatcgc cagaccctga gcgacgtgat cctcaccgtg cgttacacgg
    20101 cgcgctcggg aggcagcgcg ttcgccgaga cggtgaagaa cgcggtcggc ggaacgtcgg
    20161 aacgcgcctg gctcctgaac ctcgccaccg acttctcgga cgagtggcag gcgttcctga
    20221 gcaagccgtc cgcagggatg gcgttcacgg tggagcgccg caacctgccc ggcgccagcg
    20281 agaaggaagt gaccggggtc tacctgcact acgagatggt ggaggacccg gtggacgatc
    20341 tcagccgcca ggcgatccag ctcgagagcg gtcccgcggg tccacagcaa agcaagtacc
    20401 tgctcaagcc gggcgccttc acgaaggtcg agttgccgct cctggagcca ggccaggtcc
    20461 cggcgaaggc gacgtggacg ctgatgccga cgagcgccag cgccgcggcc aagctcagcc
    20521 cccggaacct gaagacgatc gggctggtcg tcacctacgc cggcaaggcg tcgttctgag
    20581 cgcacaacct ggctggcaac gtcggttcct gccctgtgag ggatgcgaag atccccgcgg
    20641 gcagccccgg ccgcggtaac gtggacgcat gacgaccatc gcgcaccgga tctgcccgtt
    20701 ctgcgaggcg tgctgcggcc tggagctcgc catcgaggac ggcaagatca agacgatccg
    20761 cgggtacgac gacgacgtgc actcggccgg ctttctctgc cccaagggcg ccgcgctacg
    20821 cgacctgcac gaggatcccg accggctgcg cgcgccgctg atcaagcgcg gcggcacgca
    20881 cgtcgaggtg agctgggacg aggcgttcgc cgagatcgag cgccggctgg tgccgatcct
    20941 ggcggagcac ggcaaggacg cggtcgcgat ggtgatcggc aacccgtcgg tccacaagct
    21001 cagcctgacg ctggccacgg cgcggctgac ccgggcgatc ggcacgaccc acgtgttctc
    21061 ggcgtcgacg ctcgatcaga tgccgaagca gctgtcggcg gggctgatgt tcggccactg
    21121 gctgtcgatc ccggtgcccg acctcgcgcg caccgacctg ctcgtggtgc ttggcgccaa
    21181 cccggtggtg tcgaacggca gcctgtggac ggtgccggac ttccgcggca aggcgaaggc
    21241 gatgcaggcg cggggcggca agctgatcgt gatcgacccg cggcggaccg agaccgccga
    21301 gctggcggac cagcacctgt tcatccgccc gggcggcgat gcgttcctcc tgctcggcat
    21361 cgtgcacacg ctgttcgccg accggcgggt ccggctcggg cggctggccg agcacgtcga
    21421 gggcctggcg gcgctcgagg cggcggtcgc cgagttcgcg cccgagcggg tcgccgggcg
    21481 ctgcgggatc gaggc
//
LOCUS       DASVOR010000611         5567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_122588,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000611 DASVOR010000000
VERSION     DASVOR010000611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122588"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /locus_tag="VF516_12100"
     CDS             complement(<1..916)
                     /locus_tag="VF516_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108462.1"
                     /translation="MGSIAETASHVAAERGWLVASPDGSHAAVVEPGRIVIVELRRGS
                     QVAEIGAAAALDHTDVAWIGSGARLLVLSRRGTHSTVHLIDVDGPRLRVEIQIEGTMR
                     IGATVGAQALVIGTSSMAVLTAGEAHLTPYQFPGRSLPSAAGIAGQQLIVAIAGAIEE
                     WDLLQRTPRKRLRLPRQAAIVQLGGTDRLVWMTTQQDPARIDVIPQINRGQPRAHELP
                     EPIAQVAGHPQHDVLACIGRDTGRLYLVDLDGKAALRTVDVPGLDRVDSAALFAGPAL
                     GILAARAGQPLAVIGLDGRPAVVAPTAGR"
     gene            1159..2508
                     /locus_tag="VF516_12105"
     CDS             1159..2508
                     /locus_tag="VF516_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108463.1"
                     /translation="MNKLIENARDIEQELVWLRQVIEIRLQLHTGAEARTGDVLDVTP
                     PGLEGSSSEYAGFVRRHGLSFAERLAVVLALVPHVRPRLLDSFFLRNASTDRRFTEFG
                     CVPGRTEGELLPTGETLAFLLGDEDLPLRFSVQLLFDPAHPFARHGILGPGPTAGDDV
                     PILKAPLRLSEEMVSLFTTGQPLRPAFGTSFPAQLLDAPGTWEDLVLHPSTRKQIDEI
                     RTWIEYGQALMEDWGMATRLRPGYRTLFYGPPGTGKTMTACLLGKSTGRDVYKVDIAL
                     VSSKYIGETEKNLARVFDRAEHKGWILFFDEADALFGKRGETRDAHDRYANQEIAFLL
                     QRIETFDGISILASNLRDNIDDAFARRFESVIEFPMPRVEERLVLWRQGFSPKAQLAP
                     SVDLPKLARDHVLSGGAIMNVIRHVSLQALKDGGRAVVMDDLLQGIRREYTKEGKSL"
     gene            complement(2548..4659)
                     /locus_tag="VF516_12110"
     CDS             complement(2548..4659)
                     /locus_tag="VF516_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8108464.1"
                     /translation="MRIAVKQESSSEHTSSTKGAGHTPSRASHTAAAPVTETGADAAE
                     AEPSGRKLERTFGAAIFERPHSEPSRGRTPVQRAAADRGGVRAEPGHIPPSHQDSAPG
                     SEDDRLSDRTIHEAAWLGTRGLGGPLPYLGQIQRAFGRHDVAGVVAHTDDRAAQGARA
                     MGASAFTMGNHVAFAGLPSLHTAAHEAAHVVQQRGGIQLPGGVGASGDRHERHADAVA
                     EAVVSGRSAEPLLDADARSTAVAWGGPADGGSAAIQLAVAPGPRSPQTAGAAPPEAQR
                     ATPAGTAHATPEAAAPAAELEGPPEELVQLTEPSEHAQLASPDVGMTKTVADTFRDIL
                     DHWESFQFDERYIEQLTSEQAALQQRLHDVRAPFKNQRQRLVQIGRLLARYLAMRPKA
                     RQALAGAQAVAELDAQELELEARGVVVRTANEKTAGWTNRQQRAIQQQIHALDRRKPA
                     KGSEEQTLVDRIGAISSIRNTARKNFADAKQWINRVRTGYRGTATGTQDANELAAGEL
                     ASMSTAALCNVISYWLYGTATKRETRSFPAYFREELQRGLISAQGVRYKEKLIAVKGI
                     YYGTGSQEWRHRWGMRSLVHDRIPLGDRRREAERNDFLSSGATLAISYQAFSGRTPHH
                     FLLIYKDTDGWHNLDHTSTSPARRFGATSWSQVFGVYFNDALDPVGLRQLWESFFGNS
                     AESLLKQLFPRAAPPEAPAEH"
     gene            complement(4696..>5567)
                     /locus_tag="VF516_12115"
     CDS             complement(4696..>5567)
                     /locus_tag="VF516_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX8108465.1"
                     /translation="PAPREPAPALHEPPPSAPREPSAPAPAEPALTAASQAPSPAPHA
                     QEPSGLELDLSGSREGWNKHVEEYQVRDEAKLSAETKKPEFGPESSERGGTEHHTGMH
                     FHEYSAAETAIGLRIDWDPQAGRPRKVTYNFTSAAAEVSSQATDRSFHQEPRLKGESA
                     QSREKAYTKSGFERGHLAQREAGKVDPAIDAALGLKKPTGPEVERALDVLTNVAPMTP
                     TLNKGNAWRSAETRTAQFALQEGYVTVEITPIYDAQPPRLSDGTPIPRQFRRIIKSGP
                     TGNVLENLLFDNK"
BASE COUNT          745 a         1908 c         1956 g          958 t
ORIGIN      
        1 accggcccgc cgtcggtgcg accaccgcag ggcgcccgtc gagcccgatc accgcgagcg
       61 gctggccggc gcgcgcggcg aggatcccca gcgccggtcc ggcgaacagc gccgcgctgt
      121 cgacccggtc caggcccggg acatcgaccg tgcgcagcgc ggccttgccg tcgaggtcca
      181 ccaggtacag ccgcccggtg tcgcggccga tgcaggccag cacgtcgtgc tgggggtggc
      241 cggcgacctg cgcgatcggc tcgggcagct cgtgggcgcg gggctggccg cggttgatct
      301 gcgggatgac gtcgatccgc gccgggtcct gctgggtggt catccagacc agccgatcgg
      361 tgccgccgag ctggacgatc gccgcctggc gcggcagccg cagccgcttg cgcggggtgc
      421 gctgcaggag gtcccactcc tcgatcgcgc cggcgatcgc gacgatcagc tgctgcccgg
      481 cgatgccggc cgcgctcggc agcgagcggc cggggaactg gtacggcgtg aggtgggctt
      541 cgccggcggt cagcacggcc atgctgctcg tgccgatcac cagcgcctgg gcgccgaccg
      601 tggcgccgat ccgcatcgtg ccctcgatct ggatctcgac ccggaggcgc ggcccatcga
      661 cgtcgatcag gtgcacggtg ctgtgcgtgc cgcggcgcga cagcacgagc aggcgcgccc
      721 ccgacccgat ccaggcgacg tccgtatggt ccagcgcggc cgcggcgccg atctcggcga
      781 cctggctgcc gcgccgcagc tcgacgatca cgatccggcc gggctcgacc accgcggcgt
      841 ggctgccgtc cggcgacgcg accagccagc cgcgttcggc cgcgacgtgg ctcgcggtct
      901 cggcaatgct tcccaccctg cgaacgtagc gcggatcgct cgacccgggc ccggatcggc
      961 ggcacaaccg gcccggggca gcgccgttgg cctcgaccca cccggcagtg caatggcttc
     1021 gtcaccacgg ggcatgtgcg caagcgatgc gcgacatcgc ggcgaccgag gtcgcgccgt
     1081 ccgcgcgccg gcggtggcct gttctctgca tggctgcgtg gtgcagttcc gaggtgaatg
     1141 ccggagcctg ctgcgcccgt gaacaagctc atcgagaacg ctcgcgacat cgaacaggag
     1201 ctcgtctggc tgcggcaggt gatcgagatc cgcctccagc tccacaccgg cgcggaagct
     1261 cgtaccggcg acgtcctcga tgtcacgccg cccggactcg aggggtcgag ctcggagtac
     1321 gccggtttcg tgcggcgtca tgggctgtcg ttcgccgagc gcctggcggt cgtgctcgcg
     1381 ctggttccgc acgtccggcc caggctgctc gattcattct tcctgcgcaa tgcgtcgacc
     1441 gatcgcaggt tcaccgagtt cggctgcgtg ccggggcgga cggaaggaga gctgctgccg
     1501 accggggaga cgctggcgtt cctgctgggg gacgaggatc tgccgttgcg gttctcggtc
     1561 cagctcctgt tcgatcccgc gcatccattc gcccgccacg gcatcctcgg gccggggccc
     1621 accgcgggag acgatgtgcc gatcctgaag gccccgctgc gactgtccga ggagatggtc
     1681 agcctgttca cgaccggcca gccgctgcgc cctgcgttcg gcacgagctt ccccgcgcag
     1741 ctgctcgacg ccccgggcac ctgggaggat ctggtgcttc atcccagcac gcgcaagcag
     1801 atcgacgaga tccggacctg gatcgagtac ggccaggccc tgatggaaga ctggggcatg
     1861 gccacgcggc tgcgcccggg ctatcgcacc ctgttctacg gcccgccggg caccggcaag
     1921 acgatgaccg cctgcttgct cggcaagtcg accggacgcg atgtctacaa ggtggacatc
     1981 gcgctcgtca gctcgaagta catcggcgag accgagaaga acctcgcgcg ggtcttcgat
     2041 cgagccgagc acaagggctg gatcctgttc ttcgacgagg cggacgcgct gttcggcaag
     2101 cgcggtgaga cccgcgacgc gcacgatcgc tacgccaacc aggagatcgc gttcctgctc
     2161 cagcgcatcg agaccttcga cggcatctcg atcctggcgt cgaacctgcg cgacaatatc
     2221 gacgacgcgt tcgcgcgccg cttcgagtcc gtgatcgagt ttccgatgcc ccgcgtggag
     2281 gagcgcctcg tgctgtggcg ccaggggttc tcgcccaagg cgcagctggc cccgtcggtc
     2341 gacctcccga agctggcccg ggaccacgtg ctgtcgggcg gcgcgatcat gaacgtcatc
     2401 cgccatgtct cgctgcaggc gctcaaggac ggcgggcgtg cggtggtgat ggacgatctg
     2461 ctgcagggca tccgcaggga atataccaag gaagggaagt cgctctgact acgccccgag
     2521 acactccgat cagcctgcag cagtcagtca atgctccgcc ggcgcttcgg gtggtgccgc
     2581 tcggggaaat agctgcttca acagcgactc ggcgctgttg ccgaagaacg attcccacag
     2641 ctgccgcagc ccgaccgggt cgagggcgtc gttgaaatac acgccgaaaa cctgcgacca
     2701 gctggtggca ccgaaccgcc gagctgggct ggtcgacgtg tgatccaggt tgtgccagcc
     2761 atccgtatcc ttgtagatca gcaggaagtg gtgtggagtc ctgccgctga acgcctgata
     2821 ggagatggcc agcgtcgcgc cgctcgacag gaagtcattg cgctctgctt cgcgacggcg
     2881 atcgcccagc gggatccggt catgcaccag agatcgcatt ccccagcggt gacgccattc
     2941 ttgactcccg gtgccatagt agatcccctt cacggcgatg agcttttcct tgtagcggac
     3001 accttgtgcc gagatgagac ctcgttgtag ctcctcgcgg aagtacgccg ggaacgagcg
     3061 ggtctcacgc ttggtggccg taccgtacag ccagtacgag atgacgttac acagggcagc
     3121 tgtcgacatg ctcgcgagct caccggccgc gagctcgttg gcatcttgtg tccccgtcgc
     3181 cgtgccgcga taaccggttc gcacccggtt gatccattgc ttggcatctg caaagttctt
     3241 tcgcgctgtg ttcctgatgc tggagatcgc gccgatccgg tcgacgaggg tctgctcctc
     3301 ggagcccttc gcgggcttgc gcctgtcgag cgcatggatc tgctgctgga tcgctctttg
     3361 ctgtcgattc gtccagcccg cggtcttctc gtttgcggtg cgaacgacca ccccccgagc
     3421 ctccagctcg agctcttgcg cgtcgagctc ggcgaccgcc tgcgcacccg caagcgcctg
     3481 cctggccttc ggtcgcatgg caagatacct cgcaagcaga cggccgatct gaaccagacg
     3541 ctgccgctgg ttcttgaacg gtgccctcac atcgtgcagt cgttgctgca atgccgcctg
     3601 ctcgctcgtg agttgttcga tatagcgctc gtcgaactgg aacgactccc agtggtccag
     3661 gatgtctcgg aacgtgtcgg cgaccgtctt cgtcatgccg acgtcgggac tcgcgagctg
     3721 tgcgtgctcc gacggttccg tgagctggac gagttcctcg ggcgggccct cgagctctgc
     3781 tgcgggcgct gcggcctcgg gcgtggcatg tgccgtgccg gccggtgtcg cgcgctgcgc
     3841 ctcggggggc gccgcacccg ctgtctgcgg cgacctgggt cccggtgcca cggcgagctg
     3901 gatcgcggca gatccgccgt ccgcgggtcc gccccacgcg acagccgtcg agcgagcgtc
     3961 tgcgtcgagc agcggttcgg cggagcgccc cgacaccacc gcttcggcga ctgcatccgc
     4021 gtgtcgttcg tggcggtcac cggacgcacc gacaccgccg ggaagctgga tcccgccgcg
     4081 ctgctggacg acgtgcgcgg cttcgtgggc ggctgtatgg aggctgggga gtcccgcgaa
     4141 cgccacatgg tttcccatcg tgaacgccga tgcgcccatc gccctggcac cctgtgcggc
     4201 gcggtcatcg gtgtgcgcca cgacgcccgc gacgtcgtgc cgaccgaatg ccctctggat
     4261 ctgcccgagg tagggcaggg ggccgccgag gccgcgcgta ccgagccagg cagcctcgtg
     4321 gatcgtgcgg tcggacagcc ggtcgtcttc gctccccgga gcgctgtcct gatggctcgg
     4381 cgggatgtgc ccgggctccg cgcgcacgcc gccgcgatcc gccgctgcgc gctgcacggg
     4441 cgtgcgcccg cgtgacggct cggaatgcgg acgctcgaag atcgctgccc cgaacgtgcg
     4501 ctccagcttg cgtcccgagg gttcggcctc ggctgcgtcg gcgcccgttt cggtgaccgg
     4561 agccgccgcg gtgtgcgatg cacgagatgg cgtgtgcccg gctcccttcg tcgaggaggt
     4621 gtgctccgag ctcgactcct gcttcaccgc aatccgcatc gggtactcct tcgcgacgag
     4681 ccgtctccgc cgagatcact tgttgtcgaa caacaggttc tccagcacgt taccggtcgg
     4741 gccgctcttg atgatgcggc ggaactggcg gggaatcggt gtgccatcgc tgagcctggg
     4801 cggctgggca tcatagatcg gcgtgatctc cacggtgacg tagccttcct ggagcgcgaa
     4861 ctgcgccgtc cgggtctcgg cggagcgcca ggcgttgccc ttgttcaagg ttggggtcat
     4921 cggcgcgacg ttggtcagca cgtcgagcgc gcgctccacc tcggggccgg ttggcttctt
     4981 cagtccgagt gccgcgtcga tcgcgggatc gaccttgccg gcctcgcgct gggcgagatg
     5041 gcctcgctcg aagcccgact tcgtgtaggc cttctctcgg gattgcgcgg attctccctt
     5101 gagccgcggc tcctggtgga agctgcggtc ggtggcctgc gacgagacct cggcggcggc
     5161 ggacgtgaag ttgtaggtga ccttgcgggg ccggccggcc tgcggatccc agtcgatgcg
     5221 gaggccgatc gcggtctccg cggcgctgta ctcgtggaag tgcatgccgg tgtggtgctc
     5281 ggtgccgcct cgctcgctcg actccgggcc gaactcgggc ttcttggtct cggcgctcag
     5341 cttggcctcg tctcgcacct ggtactcctc cacgtgcttg ttccagccct cgcgactgcc
     5401 gctgaggtcg agctcgagtc cgctgggttc ctgcgcgtgc ggggccggcg acggtgcctg
     5461 gctggccgcg gtcagggccg gctccgccgg tgcgggagcc gacggttccc ggggtgcgga
     5521 gggcgggggc tcgtgcaggg cgggcgcggg ctcgcgcggg gcgggcg
//
LOCUS       DASVOR010000612         4444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_93933,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000612 DASVOR010000000
VERSION     DASVOR010000612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4444
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93933"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1516)
                     /locus_tag="VF516_12120"
     CDS             complement(113..1516)
                     /locus_tag="VF516_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108466.1"
                     /translation="MIVPRILTLALSCAAASAGASPRSDPTAGRAVFTGATMPHATSI
                     GVDPAALGLGSVDELYIAFTGVLDQLHVALDRGPDNQAPGGQAQDVEVSPGAMLAFIY
                     HLAGDSLTLGFEAHTAPPETFPSHQSALQYHVAGGGERDWLASVGASYKVADILFVGA
                     SLSHQNTFLRLQYTRDAALESPEGPGAALCGGAPCGIGNPDAAQKWDVDVRSPSLSTS
                     NLKVNVGVLVQIAHDVWLGIGYHTPPGFGVESELAGHVAITNPDLTVLREQSVVDIQL
                     PASADAELRARLPAGLDLHVGGRWEDLSRLSAYDVRAYSSTSVANGIPEWTERPRGMH
                     DAFAGWAGVEQVDRGQSWLLGARGGFETASVSAARTSPLTVAPASVTLDLGAQLRVSR
                     GLIAQLSYGLQYFPTLQVSRSDFDPEFRVACMQSNYDYTTAACAATRNGYAIASAAGS
                     YDRIEHALRLGLRYDLP"
     gene            1551..2867
                     /locus_tag="VF516_12125"
     CDS             1551..2867
                     /locus_tag="VF516_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108467.1"
                     /translation="MLARVLPIAVAAFTFGAAGALALGAAGCEKLDHENIDKWSHTSK
                     GPAKLLQAVTSDRADPDLSAHAAANLIRRDDDREVYAAFEAMPAARRAPVIARLAPRL
                     WDIARVESERELPGKPQAGAKDALVRVRRWADEPTRQQIDGYLIDWYCVASYEDRARS
                     GFATGASVMRMVGPPAGRKLIAVVNGVIAAPGQDRVRNRIGDQLLLGLAATGYPDAVK
                     YVLDLARMDRGDPTLVSRALSALYKAYVAPDGFEPADRAALLPSLPALVAIARDDRMS
                     GRPANDAVALIQAVGPPSCLAPLLGMIGAPHPSARFKFVVVQSALRCGGPGAVVEVVR
                     ALPAGGYAQDEIDGAISGEIARMTPRDQAQAAARALLGEPSTLARWVGIEALGAMKAS
                     DDAPRVEALASHQEPLVGYWGQRGQGKPDPTLGQRAHEISTLLGVK"
     gene            2974..3795
                     /locus_tag="VF516_12130"
     CDS             2974..3795
                     /locus_tag="VF516_12130"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8108468.1"
                     /translation="MDQSKKTMRHFYCRDVLWETFEQMANDFDCSIDYLVNEAMRYYA
                     RSKNYQSPGGPPPTQGGPPPTQGVPAVVPNAGPAMNNTGGPTSYSGGPAKPGNGMRGQ
                     PGTQPPPSGAGMQGMRGRAQPPTPGYQGQGHQNNLNVSARTASPTGVPGGMNAMSHPS
                     VQAGPATGASPMPMGVGGGPTLFLIYNGQRYPVTKDQFIIGRGSKTSDLPIKDGNISR
                     KHAAVIRRNGTFYIKDLGSTNGIDYKGMRIDNKRIDEGDVFHLCDYELRFTYRAD"
     gene            4180..>4444
                     /locus_tag="VF516_12135"
     CDS             4180..>4444
                     /locus_tag="VF516_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108469.1"
                     /translation="MDAELKRRVEALRRPLELAAADGFAGVRTVRGLGHALRAACDGV
                     LARIAADQQATAITGLDTWRTTLGRWEQLDEQQQAIEVARGMRL"
BASE COUNT          667 a         1598 c         1586 g          593 t
ORIGIN      
        1 gcgccggcgg ccgaccagcc gcacaggacc gccgccggcc gcgagcgcct cgcacagccg
       61 gcgcccgacc acgccggtcg cgccgaacac cgcgatccgc ggcgcgccac cttcagggga
      121 ggtcatagcg cagcccgagc cgcagcgcgt gctcgatccg atcgtagctg cccgccgcgc
      181 tggcgatcgc gtagccgttg cgggtcgccg cgcacgccgc ggtcgtgtag tcgtagttgc
      241 tctgcatgca cgcgacccgg aactcgggat cgaagtcgct ccggctgacc tgcagcgtcg
      301 ggaagtactg caggccgtac gacagctgcg cgatcagccc gcgcgacacc cggagctggg
      361 cgccgaggtc gagcgtgacc gacgccggcg ccacggtcag cggcgaggtc cgcgcggccg
      421 acaccgacgc ggtctcgaac ccgccgcgcg cgccgagcag ccagctctgc ccgcgatcga
      481 cctgctcgac gccggcccag cccgcgaacg cgtcgtgcat cccgcgcggc cgctcggtcc
      541 actccgggat gccgttggcc accgaggtgc tgctgtaggc gcgcacgtcg tacgcgctca
      601 gccgcgacag gtcctcccag cgcccgccga cgtggaggtc caggccggcg ggcagccgcg
      661 cgcggagctc ggcgtcggcg ctggccggga gctggatgtc gaccaccgac tgctccctca
      721 gcacggtcag gtcggggttc gtgatcgcga cgtggccggc gagctcgctc tcgaccccga
      781 aaccgggcgg cgtgtggtag ccgatgccca gccatacatc gtgggcgatc tggaccagca
      841 cgccgacgtt gaccttgagg ttcgaggtcg acaggctcgg cgagcgcacg tcgacgtccc
      901 atttctgggc ggcgtcgggg ttgccgatcc cgcacggtgc gccgccgcac agcgcggcgc
      961 cggggccctc ggggctctcg agcgccgcgt cgcgcgtgta ctgcagccgg aggaacgtgt
     1021 tctggtgcga caggctcgcg ccgacgaaca ggatgtcggc gaccttgtag ctcgcgccga
     1081 ccgacgcgag ccagtcgcgc tcgccgccgc ccgccacgtg gtactgcagc gcggactggt
     1141 gcgacggaaa cgtctcgggc ggcgcggtgt gggcctcgaa gcccagcgtc aggctgtcgc
     1201 cggcgaggtg gtagatgaac gcgagcatcg cgccggggct gacctcgacg tcctgggcct
     1261 gcccgcccgg cgcctggttg tccggtccgc gatcgagcgc cacgtgcagc tggtcgagca
     1321 cgccggtgaa cgcgatatac agctcgtcga ccgagccgag cccgagcgcg gcgggatcga
     1381 ccccgatcga cgtcgcgtgc ggcatcgtgg cgccggtgaa caccgcacgg ccggccgtcg
     1441 gatcgctgcg cggcgacgct cccgccgacg cggccgcgca cgacagcgcc agcgtgagga
     1501 tgcgtggaac gatcatcgcg cggaacgtat caccacgtta agctcccgcc atgctcgcgc
     1561 gcgtgttgcc gatcgccgtg gcggccttca ctttcggcgc cgccggtgcg ctggcgctcg
     1621 gcgcggcggg ctgcgagaag ctcgaccacg agaacatcga caagtggtcg cataccagca
     1681 aggggccggc gaagctgctc caggcggtga ccagcgaccg cgccgacccc gatctgtcgg
     1741 cccacgccgc ggcgaatctg atccggcgcg acgacgaccg cgaggtctac gccgcgttcg
     1801 aggccatgcc ggccgcgcgc cgcgcccccg tgatcgcccg gctcgcgccg cggctgtggg
     1861 acatcgcgcg ggtcgagagc gagcgcgagc tgccgggcaa gccccaggcc ggcgccaagg
     1921 acgcgctggt ccgcgtccgg cgctgggccg acgagccgac ccggcagcag atcgacggct
     1981 acctgatcga ctggtactgc gtcgcctcgt acgaggaccg cgcccggtcc ggcttcgcca
     2041 ccggcgcgtc ggtgatgcgg atggtcggcc cgccggccgg ccgcaagctg atcgccgtcg
     2101 tcaacggcgt gatcgcggcg cccggtcagg atcgcgtccg caaccggatc ggcgaccagc
     2161 tgctgctcgg cctggcggcg accgggtacc ccgacgcggt gaagtacgtg ctcgacctcg
     2221 cgcggatgga tcgcggcgat ccgacgctgg tgagccgcgc gctgtcggcg ctctacaagg
     2281 cctacgtcgc acccgacggc ttcgagcccg ccgatcgcgc cgcgctgctg cccagcctgc
     2341 cggcgctcgt cgcgatcgcc cgcgacgacc ggatgtcggg ccggccggcc aacgacgcgg
     2401 tcgcgctgat ccaggcggtc ggtccgccga gctgcctggc gccgctgctg ggcatgatcg
     2461 gcgcgccgca ccccagcgcc cggttcaagt tcgtcgtcgt ccagagcgcc ctgcgctgcg
     2521 gtgggcccgg ggccgtcgtc gaggtcgtcc gggcgctgcc cgccggcggg tatgcgcagg
     2581 acgagatcga cggtgcgatc tcgggcgaga tcgcccgcat gacgccgcgc gaccaggccc
     2641 aggccgccgc ccgggcgctg ctcggcgagc cgtcgacgct ggccaggtgg gtggggatcg
     2701 aggcgctggg tgcgatgaag gcatccgacg acgcacctcg ggtcgaggcg ctggcatccc
     2761 accaggagcc tctcgtcggc tactggggcc agcgagggca gggcaagccc gaccctaccc
     2821 tgggacagcg ggcccacgag atttcgaccc tgctcggcgt caagtgaccc gcgatagtcg
     2881 cgcccgctcg tgggatgtag tatcgtaaag tacagccggg aagaaagggc aagaggccca
     2941 tcgtttcgtc agttccgggg agcagccaag cgaatggatc agagcaagaa gacaatgcgg
     3001 cacttctact gccgcgatgt cctgtgggag acgttcgagc aaatggcgaa cgacttcgat
     3061 tgctctatcg actacctcgt caacgaggcg atgcggtact acgcgcggtc gaagaactac
     3121 cagtcgcccg gcgggccgcc gcccacgcag ggcgggccgc cgcccacgca gggtgtgccc
     3181 gcggtcgtcc ccaacgccgg accggcgatg aacaacaccg gcggacccac ctcgtactcg
     3241 ggcggtccgg ccaagccggg caatggcatg cgcggccagc ccggcacgca gccgcctccg
     3301 tcgggtgccg ggatgcaagg catgcgtggc cgtgcgcagc cgccgacccc gggctaccag
     3361 ggccagggtc atcagaacaa cctcaacgtg agcgcccgta cagcgtctcc gaccggtgtt
     3421 cccggcggga tgaacgccat gtcgcatcca tcggtgcagg cgggaccggc caccggcgcc
     3481 tcgccgatgc cgatgggcgt gggcggcggg ccgacgctgt tcttgatcta caacggccag
     3541 cgctacccgg tgaccaagga ccagttcatc atcggccgcg gcagcaagac ctcggacctg
     3601 ccgatcaagg acggcaacat ctcgcgcaag cacgccgccg tgatccgccg caacggcacc
     3661 ttctacatca aggatctcgg ctcgaccaac ggcatcgact acaaggggat gcgcatcgac
     3721 aacaagcgca tcgacgaggg cgacgtcttc cacctgtgcg actacgagct gcggttcacg
     3781 taccgggcgg actgaggcaa ggtcctgcag ggcggcgcag cgcgcggcga tcgcgtcgaa
     3841 tgtgctggca catgccacga cgcgctcgcg tagccgtcat cgacgacctt gaccttgacc
     3901 ccacccttga catcgacggc aacgtagacg tctgacccca cccttgacct tgcacgttga
     3961 ctcgacgaac gcgatccgag gtcgacgtcg aggtcgacgg tggggtcgac gtcgaacgtc
     4021 gccctcgacc tcaacgttcg gggtcgacgt caaccgtcaa cgtcaacctc cgcttgcgtc
     4081 ccggatcact cctctccatc gaggcctgga gcgagatctt cggaggcccg gcgggcggcc
     4141 gtcaatcaag atgacacccc cgggtgctag cttggcgtcg tggatgccga gctgaagcgt
     4201 cgggtcgagg cgctgcggcg gccgctggag ctggccgccg ccgacgggtt cgccggcgtg
     4261 cgcacggtgc gcggcctcgg ccatgcgctg cgcgcggcgt gcgatggcgt gctcgcccgg
     4321 atcgcggccg accagcaggc gacggcgatc accgggctcg acacctggcg gacgacgctg
     4381 ggccgctggg agcagctcga cgagcagcag caggcgatcg aggtggcgcg cggcatgcgc
     4441 ctgc
//
LOCUS       DASVOR010000613         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_93940,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000613 DASVOR010000000
VERSION     DASVOR010000613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93940"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF516_12140"
     CDS             complement(<1..204)
                     /locus_tag="VF516_12140"
                     /inference="COORDINATES: protein motif:HMM:NF013317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HEX8108470.1"
                     /translation="MNRPALPAERGRESPLWLDGSEGEGGGQILRTSLALVMITGRPL
                     EMSRIRAGRARPGLRRQHLACVEA"
     gene            complement(278..1504)
                     /locus_tag="VF516_12145"
     CDS             complement(278..1504)
                     /locus_tag="VF516_12145"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HEX8108471.1"
                     /translation="MASYDLIETDGGVPIKAWTRGVVVEDAAKKQLENVAKLPIVFHH
                     VAAMPDVHFGIGATVGSVVPTERAIIPAAVGVDIGCGMMAVETTLTASQLPDTLRPLR
                     TAIEKAVPHGRTANGGRGDRGAWHKIPPATAAAWQELAPEYERLVDRHKALGQGNDVN
                     HLGTLGTGNHFIEVCLDERDRVWFMLHSGSRGVGNRIGTYFIELARADMKQHLRNLPD
                     ADLAYLEEGTRHFQDYVAAVDWAQRFARKNRDLMMASVVAAARQPGIGLPQFELGELA
                     VNCHHNYVEREHHFGADVFVTRKGAVRAGLGELGIIPGSMGARSFIVRGKGERESFCS
                     CSHGAGRAMSRGEAKRRFTLDDHARATEGIECRKDAAVIDETPGAYKPIDAVMAAQAD
                     LVEVVHELRQVVCVKG"
     gene            1684..1929
                     /locus_tag="VF516_12150"
     CDS             1684..1929
                     /locus_tag="VF516_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108472.1"
                     /translation="MASITIRNLDVQLEAQLQARAARHGRSLEAEIRAILRGAVALDA
                     AGSGNLAAAIADLFRPLGGVELEIPAREPMRKPPRFG"
     gene            1992..2246
                     /locus_tag="VF516_12155"
     CDS             1992..2246
                     /locus_tag="VF516_12155"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX8108473.1"
                     /translation="MSQQVSIRLPSPMLAALDRRARRRRVPRADLIRDALTAYLELPD
                     GALQRRPIDRIRNLLGAIDGLPPDLASRADDYLEDLGRPR"
     gene            2212..2649
                     /locus_tag="VF516_12160"
     CDS             2212..2649
                     /locus_tag="VF516_12160"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX8108474.1"
                     /translation="MTTSKTSAGHDDWLLDTGPLVALLSKGDSAHAACVSALERFHGH
                     LITTEPVLTEAMHLVRRQPGAQGACLDFFLQGAAVLVPMTAERLAACKALVTRYRDVP
                     MDFADATLVALADELTLGAVFTLDRRGFATYRWRKTRRFELVP"
     gene            complement(2660..>2987)
                     /locus_tag="VF516_12165"
     CDS             complement(2660..>2987)
                     /locus_tag="VF516_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8108475.1"
                     /translation="GRELSLGQWQKVALARSFMRRDADVLVLDEPTASMDAAAEVAIF
                     ERFRELTDDKIAIVISHRFSTVRMADEIIVLDRGAIMERGSHAELLAQDGRYATLFNL
                     QAQGYR"
BASE COUNT          403 a         1079 c         1022 g          483 t
ORIGIN      
        1 agcctcgacg cacgccaggt gctggcggcg cagccccggc ctggcccgcc cggcccggat
       61 ccgggacatc tcgagcggcc ggccggtgat catcaccagc gcgagcgagg tgcgcaggat
      121 ctgcccgccg ccttcacctt cggatccgtc gagccagagg gggctttccc gccccctctc
      181 ggccggcaga gccggccgat tcaccccgcc gcgcgtcgcg gcgcgcggcc ctgcacctga
      241 tgactctgtg ctcacctgga actcctccgg gcaccgccta gcccttgacg cacacgacct
      301 ggcgcagctc gtggacgacc tcgaccaggt cggcctgggc ggccatcacg gcgtcgatcg
      361 gcttgtaggc gccaggggtc tcgtcgatca ccgcggcgtc cttgcggcac tcgatgccct
      421 cggtggccct ggcgtgatcg tcgagcgtga accgccgctt ggcctcgccg cgcgacatcg
      481 cgcgccccgc gccgtggctg cacgagcaga acgactcgcg ctcgcccttg ccgcgcacga
      541 tgaagctgcg cgcgcccatc gagcccggga tgatgccgag ctcgccgagc ccggcgcgca
      601 ccgccccctt gcgggtcacg aacacgtccg ccccgaagtg gtgctcgcgc tcgacgtagt
      661 tgtggtggca gttgaccgcg agctcgccga gctcgaactg cggcaggccg atgccgggct
      721 ggcgcgccgc ggccacgacg ctggccatca tcaggtcgcg gttcttgcgc gcgaaccgct
      781 gcgcccagtc gaccgccgcg acgtagtcct ggaagtgccg ggtgccctcc tcgaggtacg
      841 ccaggtcggc gtcgggcagg ttgcggaggt gctgcttcat gtcggcgcgc gcgagctcga
      901 tgaagtacgt gccgatccgg ttgccgacgc cgcgcgaccc ggagtgcagc atgaaccaca
      961 cccggtcgcg ctcgtcgagg cagacctcga tgaagtggtt gccggtgccg agcgtgccga
     1021 ggtggttgac gtcgttgccc tggccgagcg ccttgtgccg gtcgaccagc cgctcgtact
     1081 ccggcgcgag ctcctgccac gccgccgcgg tcgccggcgg gatcttgtgc cacgcgccgc
     1141 ggtcgccgcg gccgccgttg gcggtccggc catgcggcac cgccttctcg atcgccgtgc
     1201 gcagcggccg gagcgtgtcg ggcagctggc tcgcggtcag cgtggtctcg accgccatca
     1261 tgccgcagcc gatgtcgacc ccgaccgcgg ccgggatgat cgcgcgctcg gtcggcacca
     1321 cgctgcccac cgtcgcgccg atcccgaagt ggacgtcggg catcgccgcg acgtggtgga
     1381 acacgatcgg cagcttcgcg acgttctcca gctgcttctt cgcggcgtcc tccacgacga
     1441 cgccgcgcgt ccacgccttg atcggcacgc cgccgtcggt ctcgatcaga tcatagctcg
     1501 ccatgacacg acatccattt gcaccttgca tccccggggg cccgccggca cctgccagcc
     1561 tgggccgctc gcaagccgga gcggctcgcg agggcgctat tacctacggg gttctctccg
     1621 acgatgcaag cgcgatccgc taagttcgcg taggtaaacg gcgtcgagga cgacattgcc
     1681 atcatggcca gtatcacgat ccggaacctc gacgttcagc tcgaggcgca gctgcaagcg
     1741 cgggcggctc gccatggacg gtcgctcgaa gccgagatac gcgcgatctt gcggggcgcg
     1801 gttgcgttgg atgctgcggg atcgggcaac ctcgccgccg cgatcgccga cctgttccgg
     1861 ccgctcggtg gggtcgagct cgagatcccc gcgcgtgagc cgatgcgcaa gcctcccagg
     1921 ttcgggtgag ccgcgatcgc ctcgtacgat ggccctcccg gcgtaacccg tctgggttac
     1981 acttgtcatc catgagccag caggtctcga ttcgattgcc ttctccgatg ctcgcggcgc
     2041 tcgatcgccg ggccaggcgt cgtcgcgtac cccgcgcgga cctcattcgc gatgcactga
     2101 cggcgtatct ggagttaccc gacggtgcgc tgcaacgccg gccgatcgat cggatccgga
     2161 acttgctggg cgcgatcgac ggacttcccc cggacctggc gagccgcgcc gatgactacc
     2221 tcgaagacct cggccggcca cgatgactgg ctgctcgaca ccggtccgct ggtggcgctg
     2281 ctgtcgaagg gcgactcggc gcacgccgct tgcgtctcgg cgctcgagcg cttccacggc
     2341 cacctgatca ccacggagcc ggtgctcacc gaggcgatgc acctggtgcg ccgccagccg
     2401 ggtgcacagg gtgcctgtct cgatttcttc ttgcagggcg ccgccgtgct ggttccgatg
     2461 accgcggagc gtctcgccgc gtgcaaggcg ctggtcaccc ggtatcgcga cgtgccgatg
     2521 gatttcgccg atgcaacgct ggtggcgctc gccgacgagt taaccctggg cgcagtattc
     2581 acgctcgacc ggcgcggctt cgcgacctat cgctggcgca agacgcgtcg cttcgagctc
     2641 gtgccatgac gtggggggct cagcggtagc cctgggcctg gaggttgaac agcgtggcgt
     2701 agcggccgtc ctgggcgagc agctcggcgt gcgagccgcg ctccatgatc gcgccgcggt
     2761 cgagcacgat gatctcgtcg gccatccgca cggtcgagaa ccggtgcgag atcacgatcg
     2821 cgatcttgtc gtcggtgagc tcgcgaaacc gctcgaagat cgcgacctcg gcggcggcgt
     2881 ccatcgacgc ggtcggctcg tcgagcacca gcacgtcggc gtcgcgccgc atgaacgagc
     2941 gggcgagcgc gaccttctgc cactggccga gcgacagctc gcggccg
//
LOCUS       DASVOR010000614         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_385524,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000614 DASVOR010000000
VERSION     DASVOR010000614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385524"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..612
                     /locus_tag="VF516_12170"
     CDS             <1..612
                     /locus_tag="VF516_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014433.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA-related P-loop ATPase"
                     /protein_id="HEX8108476.1"
                     /translation="RAIPGLDDYALLGKAWYHTTEEKRGAPVWDTVVFDMPASGHSVS
                     MLRVPWVIVDTVPEGPLTRDARTIKAMLCDPARTSAVLVTLAEEMPVNEAVELETKLI
                     TLGIVPQQVLVNQVFPRHFPPGAPMSRVLDALLTEPVSLQPPLAQLAAHAALSRDRRA
                     LHERYLAELRARAKAKVDELPMLFAQTLGPVEIAKLGDRIASV"
     gene            1004..1888
                     /locus_tag="VF516_12175"
     CDS             1004..1888
                     /locus_tag="VF516_12175"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8108477.1"
                     /translation="MSEPTNQPAIEALYEQVRAVAPAAPDGPPPELRTVAPGIRVLAL
                     RTPTLPPAAHTNIYLVGPEAGPVAVIDPGSPYPDQQVILDGVLASAPPAAVLLTHHHG
                     DHVGGAVALAERWSVPIAAHPATARRLAGRVAVSHHLEDGDTAYGVTAIFTPGHAEGH
                     LCYAVGDATIAGDMVAGVGTILIDPSEGDMAVYLASLERLLARPPMVLLPAHGPEIAD
                     GHRKLSEYLAHRRMREALVVAALRDTPRPLSELVAEAYRDTPRMLWGLAERSLLAHLV
                     KLAREQRAIDHGDGRWSS"
     gene            1944..>2168
                     /locus_tag="VF516_12180"
     CDS             1944..>2168
                     /locus_tag="VF516_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108478.1"
                     /translation="MAIARDLLRRWWGLELGLAEASGAGYARTSHAVCETVRGAAAAA
                     CAGALSEIAAGFAASMQRDPVVRTCHAGLLI"
BASE COUNT          297 a          760 c          778 g          333 t
ORIGIN      
        1 cgcgcgatcc ccgggctcga cgactacgcg ctgctcggca aggcctggta ccacaccacc
       61 gaggagaagc gcggcgcgcc ggtgtgggac accgtggtgt tcgacatgcc ggcgtccggc
      121 cactcggtgt cgatgctgcg cgtgccatgg gtgatcgtcg acacggtgcc ggagggcccg
      181 ctgacccgcg acgcgcgcac catcaaggcg atgctgtgcg atccggcgcg gacctcggcc
      241 gtgctggtca cgctcgccga ggagatgccg gtcaacgagg cggtcgagct cgagaccaag
      301 ctgatcacgc tcggcatcgt gccgcagcag gtgctggtca accaggtgtt cccgcgccac
      361 ttcccgcccg gcgcgccgat gtcgcgggtg ctcgacgcgc tgctcaccga gccggtgagc
      421 ctccagccgc cgctcgcgca gctcgccgcg cacgccgcgc tgtcgcgcga tcgccgcgcg
      481 ctgcacgagc gctacctcgc cgagctccgc gcgcgggcga aggccaaggt cgacgagctg
      541 ccgatgctgt tcgcgcagac cctcgggccc gtggagatcg ccaagctcgg cgatcggatc
      601 gcgagcgtgt gagcgtgtcc cggcgcgtcc gggcgccgct cctcgccggc ggcgcgcatc
      661 gggaacagcg gctcgccgtg gatctcgagc cggtacacgc tgttctcggt ctcgacgtag
      721 atcatcttgc cgcccggctc gcccaggacg cgcttgaccg gtgtggtgat catccggtgc
      781 ccgtgcgggt cacgaaagat gaccatgccg cggccgatgt acgggtacga gatcaggtac
      841 ccggtgtaga gccgtccgct gccgccggta ggtcgctcgg acccgagttc acgaatcttg
      901 cgtgcagtca cgctgaccat tggtgcgctg gctatagttt acgagatggg cgcccccggg
      961 tcaccctctt tgcaagcgat gtcagatgcc ggtggggtaa gcgatgagtg agccgacgaa
     1021 ccagccggcg atcgaggcgt tgtacgaaca ggtgcgcgcc gtggcaccgg cggctcccga
     1081 cggcccgccg cccgagctgc ggacggtagc gccggggatt cgcgtgcttg cgcttcgcac
     1141 ccccacgtta ccccccgccg cccataccaa catctacctg gttggacccg aggccggtcc
     1201 ggtcgccgtg atcgaccccg ggagtcccta tccggaccag caggtcatcc tggacggtgt
     1261 gctggcgagc gcgccgccgg ccgcggtctt actcacccat caccatggcg accacgtggg
     1321 cggtgcggtc gcactggcgg agcgctggtc ggtgccgatc gcggcacacc ccgcgaccgc
     1381 gcgccggctc gccggccggg tcgccgtatc ccaccacctg gaggacggtg acaccgccta
     1441 cggcgtcacc gcgatcttca cacccgggca cgccgagggc cacctgtgct atgcggtcgg
     1501 cgacgcgacg atcgcgggtg acatggtcgc cggtgtcggc acgatcctga tcgatcccag
     1561 cgagggcgac atggcggtgt acctcgcgtc gctggagcgc ttgctcgcac ggccgccgat
     1621 ggtgctgctt ccggcgcacg gtccggagat cgccgatggc cacagaaaac tgagcgagta
     1681 tctcgcacac cgccggatgc gagaggcgct cgtcgtcgcc gcgctgcgcg atacgccgcg
     1741 cccgctgtcc gagctggtcg ccgaggcata tcgcgacacg ccgcgcatgc tgtgggggct
     1801 ggccgagcga tcgctgcttg cacatctggt gaagctcgca cgggagcaac gcgcgatcga
     1861 ccacggcgac gggcgctggt cgagctgacg tcgcgccgct atagtgagcc gaggtgggct
     1921 tctcgttcga tgatctttcc gacgtggcga tcgcgcgcga cctgctgcgc cggtggtggg
     1981 gactggagct cggcctggcg gaggcgagcg gtgccggcta tgcccgcacg tcgcacgcgg
     2041 tctgcgagac cgtgcgcggc gcggcggcgg cggcgtgcgc cggggccctg agcgagatcg
     2101 cggccgggtt cgccgcgtcg atgcagcgcg atccggtggt gcggacctgc cacgccggcc
     2161 tgctcatc
//
LOCUS       DASVOR010000615         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_356528,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000615 DASVOR010000000
VERSION     DASVOR010000615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356528"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..938
                     /gene="pilM"
                     /locus_tag="VF516_12185"
     CDS             <1..938
                     /gene="pilM"
                     /locus_tag="VF516_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEX8108479.1"
                     /translation="VPQTIVDGTIMDQGAVAEAVRQLWKRLRLKQKEVAIAIAGHSVI
                     IKRIAVPQMTAEQLAANIRNEAEHHIPFGRDDVEIDYHVTNPANASGQTELLLVAAKK
                     EVVSDYVQVVRDANLSPQIVDVAAFASQNGFEANYPLDPRETVVLITIGAAISNINIA
                     RSGVSLFTRDVTIGGNAFTEEIQKQLGIAADEAEAYKVGGSQTEDGVVPQEVLRVMDG
                     VSEVMAGEFQRSLDFFLATTADANVTRIMLAGGSSKVSSLHRAIERRSRLPLEVVDAW
                     RRVEIDSSLDRAYLGAHSPEALIGVGLAMRAAGDK"
     gene            940..1602
                     /locus_tag="VF516_12190"
     CDS             940..1602
                     /locus_tag="VF516_12190"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEX8108480.1"
                     /translation="MIKINLLPQRRAKRAAGSEESARPLVLGIAALVAAGAAVAVLVD
                     RPKRARLAELREANAQLRADITAKRRQLDGDGYEKLQTAEKEAKERFKSIQRLIGAKV
                     GPANVLHELGKILTTGEYPTMTEDMIKRTGNGPESDPNKRLQPDWDPSHVWLSGFTDT
                     SGDFKLEGGAATESDVTQLAKRLAVSVYFADVIPAGGERVADPQTGASYYRFTITGKV
                     VY"
     gene            1602..>2291
                     /gene="pilO"
                     /locus_tag="VF516_12195"
     CDS             1602..>2291
                     /gene="pilO"
                     /locus_tag="VF516_12195"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEX8108481.1"
                     /translation="MASAMVADFARMPTQRKVLVFVVIGALLGLLYWKFAYKALDEDL
                     DAAQVDHDNNVATSRKLAREIPAYAELRTNMARLKELIEKNQTALPSAPELPAFFETL
                     QRKFDESGVQAHKWTKRSEEPVESFIKLPVEIEVTGTFMQIKRFFASLVQRDVRPGPG
                     DDRAAAEPERIVSIENLTLINPEVKNRELTLTAKFIAVTFRQEDKAAQGPPAGGPPAA
                     PRPVAPAAPVIP"
BASE COUNT          396 a          817 c          776 g          302 t
ORIGIN      
        1 tggtcccgca gaccatcgtc gacggcacca tcatggacca gggcgcggtg gccgaggcgg
       61 tccgccagct gtggaagcgg ctgcggctca agcagaagga ggtcgcgatc gcgatcgccg
      121 ggcactcggt gatcatcaag aggatcgcgg tgccgcagat gaccgccgag cagctcgccg
      181 ccaacattcg caacgaggcc gagcaccaca tcccgttcgg ccgcgacgac gtcgagatcg
      241 actaccacgt caccaacccc gccaacgcca gcggccagac cgagctgctc ctggtcgcgg
      301 ccaagaagga ggtcgtgtcc gactacgtcc aggtcgtgcg cgacgccaac ctgtcgccgc
      361 agatcgtcga cgtcgcggcg ttcgccagcc agaacggctt cgaggccaac tatccgctcg
      421 atccgcgcga gaccgtcgtg ctgatcacga tcggcgcggc gatctccaac atcaacatcg
      481 cgcgcagcgg cgtgtcgctg ttcacccgcg acgtcacgat cggcggcaac gcgttcaccg
      541 aggagatcca gaagcagctc gggatcgccg ccgacgaggc cgaggcctac aaggtcggcg
      601 gcagccagac cgaggacggc gtggtgcccc aggaggtgct gcgggtgatg gacggcgtgt
      661 ccgaggtgat ggccggcgag ttccagcgct cgctcgactt cttcctggcg accaccgccg
      721 acgccaacgt gacccggatc atgctcgccg gcggcagctc caaggtcagc tcgctgcacc
      781 gcgcgatcga gcgccggtcg cggctgccgc tcgaggtcgt cgacgcctgg cgcagggtcg
      841 agatcgacag ctcgctcgac cgcgcctatc tcggcgcgca ctcgcccgag gcgctgatcg
      901 gcgtcgggct ggcgatgcgc gcggcgggag acaagtgaca tgatcaagat caacctgttg
      961 ccgcagcgcc gggccaagcg cgcggccggc tccgaggagt cggcgcggcc cctggtgctc
     1021 ggcatcgccg cgctggtcgc cgccggcgcc gccgtggcgg tgctcgtcga tcggcccaag
     1081 cgcgccaggc tcgccgagct ccgcgaggcc aacgcccagc tccgggccga catcacggcc
     1141 aagcggcgcc agctcgacgg cgacggctac gagaagctcc agacggccga gaaggaggcc
     1201 aaggagcgct tcaagtcgat ccagcgcctg atcggcgcca aggtcggccc ggccaacgtg
     1261 ctccacgagc tcggcaagat cctgaccacc ggcgagtacc cgacgatgac cgaggacatg
     1321 atcaagcgga ccggcaacgg ccccgagagc gatccgaaca agcggctgca gcccgactgg
     1381 gatccgtcgc acgtctggct gtccgggttc accgacacca gcggcgactt caagctcgag
     1441 ggcggcgcgg cgaccgagtc cgacgtcacc cagctcgcca agcggctcgc ggtctccgtg
     1501 tacttcgccg acgtgatccc ggccggcggc gagcgcgtcg ccgacccgca gaccggcgcc
     1561 agctactatc ggttcacgat caccgggaag gtggtgtact gatggcgtcc gcgatggtcg
     1621 cggacttcgc gcggatgccg acccagcgca aggtcctggt gttcgtcgtg atcggcgcgc
     1681 tgctcggcct cttgtactgg aagttcgcct acaaggcgct cgacgaggac ctcgacgccg
     1741 cccaggtcga tcacgacaac aatgtcgcga ccagccgcaa gctcgccagg gagatcccgg
     1801 cgtacgcgga gctgcgcacc aacatggccc ggctgaagga gctgatcgag aagaaccaga
     1861 ccgcgctgcc cagcgcgccc gagctccccg cgttcttcga gaccctgcag cgcaagttcg
     1921 acgagtccgg cgtccaggcc cacaagtgga ccaagcggtc cgaggagccg gtcgagagct
     1981 tcatcaagct cccggtcgag atcgaggtca ccggcacgtt catgcagatc aagcggttct
     2041 tcgcctcgct ggtccagcgc gacgtccggc ccggtccggg cgacgatcgc gcggccgccg
     2101 agcccgagcg catcgtgtcg atcgagaacc tcacgctgat caacccggag gtgaagaacc
     2161 gcgagctcac gctgaccgcg aagttcatcg cggtgacgtt ccgccaggag gacaaggcgg
     2221 ctcagggccc gccggcgggt ggcccgcccg cggctccccg cccggtggcc cccgcggccc
     2281 cggtgatccc g
//
LOCUS       DASVOR010000616        10752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_443968,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000616 DASVOR010000000
VERSION     DASVOR010000616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10752
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_443968"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5253)
                     /locus_tag="VF516_12200"
     CDS             complement(<1..5253)
                     /locus_tag="VF516_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108482.1"
                     /translation="MTGKARTRSLRSLHWDESKHPRNDKGEFSRKAGANWAAKVVKQI
                     GARFGDVGVGEGGREPQGRLQTGKGGLIDLSAITQGARSRAEARAPKKAILPKGTFER
                     GPSSASSSHAKPTRAIGSPESTHPDAVEPGTLKPGDVFIRNGARHEVLSGPVKGKRAG
                     LVVWRYQVRRQDGKTGPLGLTHPVQKVEPEAPKPSGARLPGGGTREAHVAETAGVTSA
                     GRAPGGATESTDDFLSRMQAKQQAARAAGGVTTSARGEQKPSVHAVMKAAGIERPDDN
                     NAKSAISRANDRLGRGEDPAAVAGTLREAARALELEREEVPEDDEQRFSELTQSIRSL
                     ERTAEALEGGAGPKGRAPGGGVVDTSRAKSETSGVTTSQARQEAAIKLGTSFSGNPYS
                     KLPTLEALTDDDFKALTPEQQDNVRFLASKIADRQTGEARYRAQVLEGRFGKWGSESE
                     QKTTFSRIVPPGGGISHEEFAARQRAKWGPGAATTQTQAADDLDGMTVVKSGRGWAVR
                     GNEADQKATKGYLYMSTTKADAEDWARTTAAKRRAMAAGNFEEAERLDRERTAALSGG
                     EGRLYRPQGSVARVSASAAKQRALQDSTDPRDNGAKIKALEEGTLDHADLYRTATAGP
                     SGQRSAVKVGRVRHTPDNPSGEFRITDRDGQLAGWVSRTNRNGEIRYSATQENYDGGT
                     ELLGWADSLAHAADVLTNGETAASSSKARNSSTARISGALFERNPARGKMVEQLDQEA
                     AQREVSRAREALKNPNLGPGMRAAHERTIAEAGKVQVYDVETGKTREVSRAEAAAGVQ
                     RHARSLGAGTPAAAVGERKAAAVATGQAGTPVKPAKPKIYEWSNVRQIPASSLEVGDE
                     FIKPTTGSAFTSHGDGEVQGHGFQPLEGTKFTVTAREGNTITARDESGRELTQTIPGD
                     RQPNVLRVGTEGPNARHERRIAEMRREAEAKSAALSAQDRATRDGVAPTDGRPNVSAL
                     LKMQGRNRDYDLGTSRGREKTMTPDIVRAGNGYMDDQRASAEIMNPLDGTGDYRYRIY
                     SDATGATLEEGTGRDLADVKAKVDRIWSSQRGGGMARSKGAGPVRFEARQTERPKDLS
                     RGDIGRGPFYDHTVVQINPDGTERIKIKTSSMSRAQEDAADLNAKESAAREAAGGGFD
                     TTGLDERQIAGLMTIDPERRAEVAAQFRLANQGRAERAARDAAELAGINAVGMPGRGE
                     SPEAQRAKLLQLASKLEGADTSRLGNEHAAEVPGRGVNYSAYIMDGKGDRILNYSDHT
                     GFSDPTGGRLADNEVPYYMAAYNANPGYGPQALRNQARETMRGAGVESVEFRRVPNDT
                     RSEREQIDQELRDLKIARQVGMRSGRLDSRREARIRQLETRNEELRVEDRKANAKQFK
                     KGDKITSRAGESEVVSINKEGYLRVQLADGKRGWVDPADAEKVSAPSAGEEFAAGTRA
                     ALGEVDPRKGGGWNLPREAPRGSKIEFTSGGNIKVPETDRQARRELEGRPPGGTTAPA
                     ASALPKTESTPAYDKLLATEGPAALQNKTMRGVVEQMEAHGWQLKSQNPMLSLTTWEA
                     PDGRTLAIQHINTKKPTIYSSEHPNAINTRTLTFKAVLAHVVTPTPPETSPGPSVGNA
                     LRSLGLPELSRQHGMTTAPTDPYLRPLYDADRGLAPKGMYGGQGKTLAEVTAVLRRNA
                     TMQRDAAKRADELYGFGGRDMNSARREQAADTMEKVADELERLDTTHRASVAEQERVK
                     ALPPDIRPGRSALAASAAKPAARTP"
     gene            complement(5250..6128)
                     /locus_tag="VF516_12205"
     CDS             complement(5250..6128)
                     /locus_tag="VF516_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108483.1"
                     /translation="MWKEDLHPRDEYGRFRDFIPGDRPGSMGTFDKLAGGLANRAHAR
                     TMEQHEALWQKQFNYYQPLQASGQLTPEIDAQWDRDIEMRDALRERLPEAGRRGLQPH
                     DDGTWRYRDRPDVPFDEHGKRLPRSAVLGYVRVGDTGDEPAAGQSELFGGYWHKPGAR
                     LHAAGGLPELYDDNGRRLSNTTVRALAGLPVTPPTVQHRRYEGAMYRAEVQAGIRREL
                     TRYQRVPFTLPSGERSVLFSGRRVERDSPEVTGANMISPLNPTSRRRTARGQRKRQQT
                     VTWIQRVNQQIEGRMA"
     gene            complement(6136..6330)
                     /locus_tag="VF516_12210"
     CDS             complement(6136..6330)
                     /locus_tag="VF516_12210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108484.1"
                     /translation="MVTSNVEPVRDGQDIIAPKTVSGPDDLVGDAWVILSPGDPDYDM
                     WDRYLRTKEGGAARRPGGGR"
     gene            complement(6324..7925)
                     /locus_tag="VF516_12215"
     CDS             complement(6324..7925)
                     /locus_tag="VF516_12215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108485.1"
                     /translation="MARREGRLPGGRRVWKEWEHPRDDRGRFARKGGAKWAARVVRAV
                     QAGFGDTGAHDPAHSIVGQRELATGHTPGRLPGGGVLDLKKISGDASLRAMLRGAENV
                     KTRDASHLIGQYEVFDGGKYHRIDATGFENGEDYVGWYENGVLRKVARPTITVRKIPG
                     FEPTHAVGRDEHRRMDGWADDARKAPQFRASTNRDRETARAQGILKDREAKLKEATRK
                     ARARANRKYPAKEGYDKRWRDEYVEREIWMEQKNLEYAQGVVRDLEANDDEWLDKASG
                     HNGIFEGKIDRSRPLAVYGDMLHFDAYDNASFVALRTLEEGVPAELHQIVAGWMHGHR
                     TYSDQFKDIPAYKGKLGEAGIFVGAKPVSQLRGWGRTKDEKPRGWGDKHTFDDVNGVV
                     DGSFGGLFVGWTDNSKRRGEGKESHTALHEFGHALDFAIGRDGEGSHSDEWAKLHARS
                     VADAGDRMSPYYKQDGHAGPQEMWAEAFMAWSRAMRDRNDGRTPLDRLLRLRFGGALG
                     VRDQAVIDDLNEYFLKITRNAGVQW"
     gene            complement(7925..10015)
                     /locus_tag="VF516_12220"
     CDS             complement(7925..10015)
                     /locus_tag="VF516_12220"
                     /inference="COORDINATES: protein motif:HMM:NF016988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEX8108486.1"
                     /translation="MAVDAVPRAGGRSALAPKPRLSTLLQQAMRPDEAAKTSIDDILH
                     GRKALEVARERCDEADSFYDGNVGMVWASDAVRLMLDRAGVEALEDFAYAHIPVDAIA
                     KRLQISSVAVAPAEENADGQDVKEDAASSAAEKAIAKLRKDNELDAEEKRLHHEVSLY
                     GEAYLFVWPVYEDGKIVSVDMRVNSAHNVIVIYDEEDPLRPKYALKSWKRQIGDDDDK
                     AAYRATLYYPNRIERWTTEPGGNPAKEDDWYRIAEVPDVDEEDMPELAQDQFYGGEDE
                     RDEGRYGDDHRDDQADLDDNDIPNPWGRVPWFHFRNNRPVGIPEHRYAYGPQQLINKI
                     VYALAGDVDFQSLPQKYILVDPTQDDPLMNLVDPDHPDEEDDDPENTGGSSGLRHSPG
                     EIWRLFGKSVGQFEPGDPSKLIALYDRCVKAMAELTGMPQWAFSRAQGDMPSGESVRE
                     LNGDLNATVQDRRDRYDPIWEDGYEFALHMLGIDGVSVDVRWAPVERVNDLNGWQVIQ
                     AKIAAGVPPQVALEEAGYAPEQVEAWLEDATGADLGRRVALLGQIATATQALGASIVT
                     GAVSAAQVQALISGLFGGILAGTDIKLPKADDFVDPQAQQQAQMQAQQDMQRAQLDHQ
                     AQQGQATREHATATQERGQQFQSDQAEAAHQRGIESFKMQQQAGGGQPRGPGGRNGRD
                     GRRESLSKPTQGGR"
     gene            complement(10186..10386)
                     /locus_tag="VF516_12225"
     CDS             complement(10186..10386)
                     /locus_tag="VF516_12225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108487.1"
                     /translation="MARLSAKRRAKIPTSKFAIKKGRKYPVDTAKRARVALGLVGMHG
                     SAAQKKAVRSAVRRRYPSIGRR"
     gene            complement(10340..>10752)
                     /locus_tag="VF516_12230"
     CDS             complement(10340..>10752)
                     /locus_tag="VF516_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108488.1"
                     /translation="MNLHASRRRGDGRPAGDPLRPQVEPAPEVVERVSPAVVEQPAPI
                     PNDGPSMHDLVMADFQARKALGLARYKALLQSHNGRVAVIDAYQEQLDWLVYMRQMIE
                     EDPQALALLRQRNESYAQEHGQAEREATGEDPHQ"
BASE COUNT         1297 a         3812 c         3657 g         1986 t
ORIGIN      
        1 gggggtacga gccgccggct tggcggccga cgcggcgagc gcggaccgtc cgggccggat
       61 gtccggcggc agcgccttga cgcgctcctg ctcggcgacg ctggcgcggt gcgtggtgtc
      121 gaggcgctcc agctcgtcgg cgaccttctc catggtgtcg gcggcctgct cgcggcgcgc
      181 cgagttcatg tcgcgaccac cgaacccgta cagctcgtcg gccctcttcg ccgcgtcacg
      241 ctgcatcgtc gcgttgcggc gcagcaccgc cgtcacctcc gccagggtct tgccctggcc
      301 gccgtacatg cccttgggcg ccaggccccg atcagcgtcg tacagggggc gcaggtacgg
      361 gtcggtgggt gccgtcgtca tgccgtgctg acgggacagc tcgggcaggc cgaggctgcg
      421 cagcgcgttg ccgacgctcg gccccgggga cgtctccggc ggggtcgggg tgacgacgtg
      481 tgcgagcacg gccttgaacg tcagcgtacg agtgttgatc gcgttggggt gctcgctgct
      541 gtagatcgtc ggcttcttgg tgttgatgtg ctgaatggcc agggtgcggc cgtccggcgc
      601 ctcccaggtc gtgaggctca gcatcgggtt ctggctcttg agctgccagc cgtgcgcctc
      661 catctgctcg acgacgccgc gcatggtctt gttctggagg gctgccggcc cctcggtggc
      721 aaggagcttg tcataggcgg gggtcgattc ggtcttgggt agcgcggacg cggccggcgc
      781 ggtcgtgcca cccggggggc gaccctccag ctcgcggcgc gcctgccggt cggtttcggg
      841 caccttgatg ttgccgccac tggtgaactc gatcttggag cccctggggg cctcgcgggg
      901 caggttccag ccgccaccct tgcgtgggtc gacctcgccg agcgcggccc gggtgccggc
      961 ggcgaactcc tcgccagcgc tcggcgcgct gaccttctcg gcgtcggcgg gatcaaccca
     1021 tccccgctta ccgtcggcga gctgaacgcg gaggtaaccc tccttgttga tgctgaccac
     1081 ttcggactcc ccggcccgac tggtgatctt gtcgcccttt ttgaactgct tggcgttcgc
     1141 cttccggtcc tcgacccgca actcttcgtt gcgggtctcc aactgccgga ttctggcttc
     1201 ccgcctggaa tccagccgcc cggagcgcat gccgacctgg cgcgcgatct tcaggtctcg
     1261 aagctcttga tcaatctgtt cgcgctcact gcgggtgtcg ttcgggacgc gccggaactc
     1321 gaccgactcg acgccggcac cgcgcatcgt ctcacgcgcc tggttccgca acgcctgcgg
     1381 gccgtagccc gggttggcgt tataggcggc catgtagtac gggacttcgt tgtccgccag
     1441 acggccaccg gtcgggtcgc taaaaccggt gtgatcgctg tagttgagaa tgcgatcgcc
     1501 cttgccgtcc atgatgtacg cgctgtagtt caccccccgg ccgggcacct cggcggcatg
     1561 ctcgttgccg agcctcgacg tgtccgcgcc ttcgagcttg ctcgcgagct gaaggagctt
     1621 cgcccgctgc gcctcggggg actcgccacg accgggcatg ccgacggcgt tgatgccggc
     1681 cagttcggcg gcgtcacggg ccgcccgttc ggccctgccc tggttggcga gccggaactg
     1741 ggcggccacc tcggcgcggc gctccggatc gatggtcatg aggccggcga tctgacgctc
     1801 gtcaagacct gtggtgtcga aaccaccgcc agcggcttcg cgcgccgcgc tttccttcgc
     1861 gttgaggtcg gcggcatcct cctgggcgcg ggacatgctc gacgtcttga tcttgatccg
     1921 ttcggtgccg tccgggttga tctggaccac ggtgtggtca tagaacggac ccctgccgat
     1981 gtcaccccgg ctgagatcct tgggccgttc cgtctgacga gcctcgaacc ggaccgggcc
     2041 ggcgcccttg ctgcgggcca tgccgccgcc gcgctggctg gaccagatcc ggtcgacctt
     2101 ggccttgacg tcggccaggt cccgcccggt gccctcctcc agggtggcgc cggtggcgtc
     2161 gctgtaaatc cggtaccggt agtcgccggt gccgtcgagc gggttcatga tttcggcgga
     2221 ggcgcgctgg tcgtccatgt agccgttgcc ggcgcgcacg atgtccggcg tcatggtctt
     2281 ttcgcggccc cggctcgtgc cgaggtcata atcccggttg cggccctgca tcttgagcag
     2341 agcggacacg ttggggcgcc cgtcggtggg cgccacaccg tcgcgggtgg cgcgatcctg
     2401 cgcgctaagc gccgccgact tcgcctcggc ctcccgccgc atctcggcga tacgccgctc
     2461 gtgacgagcg ttcggccctt cagtgccgac ccgaagtacg ttcggctgac ggtcgcccgg
     2521 gatggtctgt gtcagctcgc ggccagactc gtcacgcgcg gtaatggtgt tgccctcgcg
     2581 ggcggtcacc gtgaacttcg ttccctccag cggctggaag ccgtggccct gcacctcacc
     2641 gtcaccgtgc gaggtgaacg cgcttccggt ggtcggcttg atgaactcgt cgcccacctc
     2701 aaggctcgat gcgggaatct ggcggacatt cgaccactcg tagatcttcg gcttggctgg
     2761 cttgacgggc gtgccagcct gcccggtcgc cactgcggcg gccttgcgct cccccacggc
     2821 cgccgctggc gttccggcac cgaggctgcg cgcatgacgc tgaacgccgg ccgccgcttc
     2881 ggctcgcgac acctcgcgag tcttgccggt ctcgacgtcg tacacctgca cctttccggc
     2941 ctcggcgatg gtccgctcgt gggcagcccg catgcccggc cccaggttcg ggttcttcag
     3001 cgcctcccgc gccctcgaaa cctcgcgctg cgcggcctcc tggtcgagct gctcaaccat
     3061 cttgccgcgc gccgggttgc gctcgaacag ggcgccgctg atcctcgcag tactggagtt
     3121 gcgcgccttg ctgctgctgg ctgccgtctc gccgttggtg agcacgtcgg cagcgtgggc
     3181 gaggctgtcg gcccagccga gcagctccgt gccgccgtcg tagttctcct gggtggccga
     3241 gtacctgatc tcgccgttgc ggttggtgcg gctcacccag ccggcaagct ggccgtcccg
     3301 atcggtgatc cggaactcgc ccgaggggtt gtcgggggtg tgccggaccc ggccgacctt
     3361 gaccgcactg cgctggccgg acggtccggc cgtcgcggtg cggtagaggt cggcgtggtc
     3421 gagggtgcct tcctccaggg ccttgatctt ggcgccgttg tccctggggt cggtggagtc
     3481 ctggagcgcc cgctgcttgg cggccgacgc ggacacccgc gccacgctgc cctgtggccg
     3541 gtacaggcgg ccctcaccgc cggagagcgc cgccgtgcgc tcccggtcga ggcgttcggc
     3601 ctcctcgaag ttgccggccg ccatggcgcg ccgcttggcg gccgtggtgc gcgcccagtc
     3661 ttcggcgtcg gccttggtgg tcgacatgta caggtagccc ttggtggcct tctggtcggc
     3721 ctcgttgccg cgcacagccc acccacggcc gctcttgacg acggtcatgc cgtcgaggtc
     3781 gtcggcggcc tgcgtctgcg tcgtggcggc acccggcccc cacttggccc gctggcgtgc
     3841 ggcaaactcc tcgtggctga taccgccacc gggaggcacg atgcggctga aggtggtctt
     3901 ctgctcagac tcactgcccc acttgccgaa gcggccctca agtacctgcg cacggtaacg
     3961 cgcctcgccg gtctgccggt cggcaatctt cgacgccagg aaccgcacgt tgtcctgttg
     4021 ctcgggcgtg agcgccttga aatcgtcgtc ggtgagcgcc tcaagcgtgg ggagcttcga
     4081 gtacgggttg ccgctgaagc tggtccccag cttgatcgcg gcctcttgac gggcctggga
     4141 tgtagtcacc ccgctagtct cggacttggc acgcgacgtg tcaactacgc cgcccccggg
     4201 ggcgcgacct tttgggccgg cgccaccctc cagggcctcc gccgtgcgct ccagagaccg
     4261 gatcgactgc gtcagctccg agaaccgctg ctcatcgtcc tccggcacct cctcgcgctc
     4321 cagttcgagc gcgcgagcgg cctcgcgcag cgtgcccgcc accgccgccg gatcctcgcc
     4381 ccgaccgagg cgatcgttgg cgcggctgat cgcgctcttg gcattgttgt cgtcgggccg
     4441 ctcgatgccg gccgccttca tgacggcgtg aacgctcggc ttctgctcgc cgcgcgccga
     4501 ggtggtcacc ccgccggccg cccgcgccgc ttgctgctta gcctgcatcc gggacaggaa
     4561 atcgtcggtc gactcggtgg cgccgcccgg cgcacggccg gccgaggtga ccccggcggt
     4621 ctcggcgacg tgcgcttcac gggtaccacc acccggcagt cgggcacctg acggcttggg
     4681 cgcctcgggc tcgaccttct gcaccggatg cgtcaggccg agcggacccg tcttgccgtc
     4741 ctggcgacgc acctggtagc gccacacgac caggccggcc cgcttgccct tgaccgggcc
     4801 ggacagcacc tcgtggcggg cgccgttgcg gatgaacacg tcgcccggct tgagcgtgcc
     4861 cggctcgacc gcgtcaggat gcgtcgactc gggcgagccg atcgccctgg tgggcttcgc
     4921 gtgactgctc gacgcgctcg acgggccgcg ctcgaacgtc cccttgggca ggatcgcctt
     4981 cttcggcgcc cgcgcctcgg cgcggctccg ggcaccctgg gtgatggccg acaggtcgat
     5041 aaggccgccc ttgccggtct gcaagcgccc ctggggctcc cgaccaccct cgccgacacc
     5101 gacgtcaccg aaccgcgcgc cgatctgctt gacgaccttg gccgcccagt tggcgcccgc
     5161 cttgcgggag aactcgccct tgtcgttgcg ggggtgcttc gactcgtccc agtgcagcga
     5221 ccgcagcgag cgggtgcgcg ctttgcccgt catgccatcc gtccttcgat ctgctggttg
     5281 acccgctgga tccacgtcac tgtctgttgc cgcttacgct gtccccgcgc ggtgcgccgc
     5341 cggctcgtcg ggttcagcgg tgagatcatg tttgcgccgg tgacctctgg cgagtctcgc
     5401 tccacgcggc gcccggagaa cagtacggac cgctcaccgg acggcaacgt gaacggaacc
     5461 ctttggtagc gggtgagttc ccgacggatg cccgcctgaa cctcggcccg gtacatggcc
     5521 ccctcatagc ggcgatgctg gaccgtcgga ggtgtgaccg gcaagccggc cagcgcgcgc
     5581 accgtggtgt tgctgagccg acgaccgttg tcgtcgtaca gctcgggcag gccaccggcg
     5641 gcatggagac gcgcgcccgg cttgtgccag tagccgccga acagttcgga ctgtcctgcc
     5701 gccggctcgt cgccggtgtc gccgacccgg acatagccga gcaccgccga acgcggtagg
     5761 cgcttaccat gctcgtcgaa cggcacgtcg ggtcgatccc ggtagcgcca ggtgccgtcg
     5821 tcgtgcggct ggaggccacg cctgcccgct tccgggagcc gctcccgcag ggcgtcccgc
     5881 atctcgatgt ctcgatccca ctgggcatcg atctcggggg tgagctgacc gctcgcctgg
     5941 aggggctggt agtagttgaa ctgcttctgc cagagcgcct cgtgttgctc catggtgcga
     6001 gcgtgcgcac ggttcgccag cccgccggcc agcttgtcga aggtgcccat gctgcccggc
     6061 cggtccccgg ggatgaagtc ccggaaccgg ccgtactcgt cccgagggtg aagatcctct
     6121 ttccacatgg ggcctctacc gccccccacc gggccggcgc gcggcgccgc cctccttggt
     6181 gcgcaggtac cgatcccaca tgtcgtagtc cgggtccccg ggcgacagga tcacccacgc
     6241 gtcgccgacg agatcgtccg gcccgctcac agtcttgggc gcgatgatgt cctgtccgtc
     6301 ccggacaggc tcgacgttgc tggtcaccac tgcaccccgg catttcgtgt gatcttcaag
     6361 aagtactcgt tcaggtcgtc gatgacggcc tggtcgcgca cgccgagcgc tccgccgaac
     6421 cgcagcctaa gcaacctgtc cagcggggtg cggccgtcgt tgcggtcacg catggcgcgg
     6481 ctccaggcca tgaaggcttc cgcccacatc tcctgggggc cggcgtgacc atcctgcttg
     6541 tagtaggggc tcatccggtc cccggcgtcg gcaaccgagc gcgcgtgcaa cttcgcccat
     6601 tcgtcggagt gcgagccctc gccgtcgcgg ccgatggcga agtcgagcgc gtgcccgaac
     6661 tcgtgcagcg cggtgtgact ctccttgccc tcaccgcgcc gcttggagtt gtccgtccag
     6721 ccgacgaaca ggccaccaaa tgagccgtcg accacgccgt tgacgtcgtc gaaggtgtgc
     6781 ttgtcgcccc agccgcgcgg cttctcgtcc ttggttctgc cccagccgcg cagttggctc
     6841 accggcttgg ccccgacaaa gatgccggct tcgccgagct tgcccttgta cgccggaatg
     6901 tccttgaact ggtcggagta cgtgcggtgg ccgtgcatcc agccggcaac gatctggtgc
     6961 agctccgccg gtacgccctc ctccagggtg cgcagcgcga caaagctggc gttgtcgtag
     7021 gcgtcgaagt gcagcatgtc gccgtagacg gcgagcggcc gactgcggtc gatctttcct
     7081 tcgaagatgc cgttgtggcc gctggccttg tccaaccact cgtcatcgtt ggcttccagg
     7141 tcccgcacga ccccctgggc gtattcgagg ttcttctgct ccatccagat ctcgcgctcg
     7201 acgtactcgt cccgccagcg cttgtcgtag ccctccttgg ccgggtattt gcggttggcg
     7261 cgggcgcggg ccttccgggt ggcctccttc agcttggctt cccggtcctt gaggatgccc
     7321 tgggcgcggg cggtctcccg gtcgcggttg gtggacgccc ggaactgggg cgccttgcgg
     7381 gcgtcgtccg cccagccgtc catgcggcgg tgctcgtcgc gaccgacggc atgggtgggc
     7441 tcgaatccgg gaatcttccg cacggtgatg gtgggacggg ccaccttgcg cagtacgccg
     7501 ttctcatacc atccgacgta gtcttcgccg ttctcgaatc cagtagcgtc gatcctgtgg
     7561 tatttgcctc cgtcgaacac ctcatactga ccgatcaggt gcgaggcgtc gcgggtcttg
     7621 acgttctcgg cgccgcgcag catcgcgcgc aggctggcgt caccgctaat cttcttcagg
     7681 tcgagcactc cgccgccggg cagccggccc ggggtgtggc cggtcgccaa ctcccgctgc
     7741 ccgacgatcg agtgggccgg gtcgtgggcg ccggtgtcgc cgaagccggc ctgcaccgcg
     7801 cgcacgaccc gggccgccca cttcgcgccg cccttcctgg cgaagcggcc ccggtcgtcg
     7861 cggggatgct cccattcctt ccagacgcgc cggccaccgg gcaggcgacc ctcgcggcgc
     7921 gccattaccg accgccctgg gtgggcttgg acaggctctc gcgccggccg tcacggccgt
     7981 tgcgcccacc cggaccgcgc ggctgtccgc cgccggcctg ctgctgcatc ttgaacgact
     8041 cgatgccgcg ctggtgggcg gcctcggcct ggtcggattg gaactgctgg ccgcgctcct
     8101 gggtggcggt ggcgtgctcg cgggtcgcct ggccctgctg ggcctggtgg tcaagctgcg
     8161 cccgctgcat gtcctgctgg gcctgcatct gtgcctgctg ctgggcctgc ggatccacga
     8221 agtcgtcggc cttgggcagc ttgatgtcgg ttccggcgag gatgccgccg aacagtccgg
     8281 agatcagcgc ctgtacctgg gccgcgctga ccgcgccggt gacgatgctc gcgcccaggg
     8341 cctgggtggc ggtcgcgatc tggccgagca gcgcgacccg gcggccgagg tcggcgccgg
     8401 tagcgtcctc cagccacgcc tcgacctgct cgggggcata accggcctct tcgagcgcga
     8461 cctggggcgg caccccggcg gcaatcttgg cttggatgac ctgccacccg ttgaggtcgt
     8521 tgacccgctc gaccggcgcc cagcgcacat cgacggacac gccgtcgatg ccgagcatgt
     8581 gcagggcgaa ctcgtagccg tcctcccaga tcggatcgta acggtcgcga cgatcctgca
     8641 cggtggcgtt gaggtcaccg ttcagctcgc gcacgctctc gcccgagggc atatcgccct
     8701 gggcgcggct gaacgcccac tgtggcatgc cggtcaactc ggccatggcc ttgacacacc
     8761 ggtcgtacag cgcgatgagc ttcgacgggt cgccgggctc gaactggccc acgctcttgc
     8821 cgaacagtcg ccagatctcg ccggggctgt ggcgcagccc ggacgatccg ccggtgttct
     8881 ccgggtcgtc gtcctcctcg tcggggtggt cggggtcgac gaggttcatc agcgggtcat
     8941 cctgcgtcgg atcgacgagg atgtatttct gcgggagcga ctggaagtct acgtcaccgg
     9001 cgagcgcgta aacgatcttg ttgatcagtt gctgaggccc gtacgcgtag cggtgctcgg
     9061 ggatgccgac cggccggttg ttgcggaaat gaaaccacgg cacccggccc cacgggttgg
     9121 ggatgtcgtt gtcgtcgaga tcggcctggt cgtcgcggtg gtcgtcgccg tagcggccct
     9181 cgtcccgctc gtcctcgccg ccgtagaact ggtcctgcgc cagttccggc atgtcctcct
     9241 cgtcgacatc gggcacctca gcgatgcggt accagtcgtc ctccttggcc gggttcccgc
     9301 cgggctcggt ggtccaccgc tcgatccggt tcgggtagta cagggtggcc cggtaggcgg
     9361 ccttgtcgtc gtcgtcgccg atctggcgtt tccacgactt gagcgcgtac ttgggcctga
     9421 gcgggtcttc ctcgtcatag atgacgatca cgttgtgcgc ggagttgacc cgcatgtcca
     9481 cgctgacgat cttgccgtcc tcgtagaccg gccacacgaa caggtaggcc tcgccgtaca
     9541 ggctcacctc gtggtggagc cgcttctcct cggcgtccaa ctcgttgtct ttgcgcagct
     9601 tcgcgatcgc cttctcggcc gcgctggagg ccgcgtcctc cttgacgtcc tgcccgtcgg
     9661 cgttctcctc ggcgggagcg acggcgacgc tggagatctg gagccgcttg gcgatggcgt
     9721 cgaccggaat gtgggcgtag gcgaagtcct ccagggcttc cacgccggcc cggtcgagca
     9781 tgaggcgcac ggcatcgctg gcccacacca tgccgacgtt gccatcgtag aagctgtccg
     9841 cctcgtcgca gcgctcgcgc gccacctcca gggccttgcg gccgtgcagg atgtcatcga
     9901 tcgaggtctt ggccgcctcg tcgggccgca tcgcctgctg gagcagggtc gacagcctgg
     9961 gcttcggggc gagcgcgctc cgaccaccgg cacgcgggac cgcgtccacc gccatgagtc
    10021 acgctccgta actactgtgt gtaaccggtc tcaccgtacg actgtgcaca gggtagccga
    10081 attgcacgat gctgcaaagt atcttgcacc gaacgcaaca acgcccccga caaatgccgg
    10141 ggggcgttgc ggggcaggcg attggaaggt cagccggtgc ctagtctacc gccgaccgat
    10201 cgaggggtac ctacgccgca ccgccgagcg gacggccttc ttctgggcgg cgctgccgtg
    10261 catgccgacg aggccgagcg ccacccgcgc ccgcttcgcc gtgtcgaccg ggtacttgcg
    10321 acccttcttg atcgcgaact tactggtggg gatcttcgcc cgtcgcttcg cgctcagcct
    10381 ggccatgctc ctgagcgtaa ctctcgttgc gctggcggag cagtgccagc gcctgcgggt
    10441 cctcctcgat catctgacgc atgtacacga gccaatcgag ctgttcctga taggcgtcga
    10501 tgaccgcgac gcggccgttg tgcgactgca acagcgcctt gtaccgggcc agccccagcg
    10561 ccttacgggc ctggaagtcg gccatgacga ggtcatgcat gctcgggccg tcgttgggga
    10621 tcggcgccgg ctgctcgacc acggccggag acacccgctc gaccacctcg ggcgccggct
    10681 cgacctgcgg gcgcagcgga tcgcccgccg gccggccgtc gccgcgccgc cggctggcgt
    10741 gcaggttcat cc
//
LOCUS       DASVOR010000617         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_385545,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000617 DASVOR010000000
VERSION     DASVOR010000617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385545"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2976)
                     /locus_tag="VF516_12235"
     CDS             complement(<1..>2976)
                     /locus_tag="VF516_12235"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8108489.1"
                     /translation="LLAGRLPFEAEDAVGWVHCHVARQPRPFDQVRPSVPRPLIDIVG
                     KLLAKLPDHRYQSALGLHHDLERCLRDWTEHGALASFPLGERDVSAEFRIPQKLYGRD
                     AECAALGDAFERAAGSGTPELVLVSGYAGIGKSSVVRELLRTVVQRRGRLIAGKFEQY
                     KRDIPYFTISQALRELALDILAEDELRIARWRQRFTQALGSNCQLIVDLVPQLGLIVG
                     PQPAVPELPLTESETRLRVVFGRLFAACATPDHPLAMFIDDMQWADSASLALITNLLT
                     DGDNRHLLIIGAYRDNEVDPAHPMVRALEAARRSAARIRDLVLGPLSEHDLGRLVADT
                     VHTSPAEAAPLTGLVRDKTGGNPFFAIQFLTALHHKRLIWFDRDAYRWQWDAARIRAE
                     GYTDNIAELVRGRLYALPGETQTALQLAACLGSTVDAATLAIAGEREVAPALSPALEQ
                     HLLLETVQGEQRTYRFPHDRVHEAAYALVPEPERARIHLAIGRRLLASTPPEELAGKV
                     FEIVSQLDRGAALIESAQERERLAELYLLAGTRAQASTAYASALGYFTAGAALLAEVP
                     EVHRPELAFALELHRAECEHLTGAFDAAEQRLTELARRADSLLDLAAVTSVRIVLYAT
                     LDRPALAAEATLEYLGRTGTAWPAHPTDDEVRCEYDRIWQQLGSRAIEDLADLPPMTD
                     PERRATTELLTLAQPPVQFADENLHSLMVCRAVNLSLEHGNSDASCWAYAALGSMLRT
                     RFGNPAAALRFGQLGFALVERRQPLRWKARIYVELGYRILPWTKQPRDGVELARRAFD
                     AAMETGDLMYAAYACNAPVTLSCLAGDPLAAILDEADAALAFMHKLKFGMMVDVVTTN
                     RQLIRSLRGMTRELGSFDDEAFDEDRFEQRLEAEPGFPACWYWIHKVQARFLAGAHAG
                     AMAAAARARPLLWKIPSFPEGAEHVFYAALACAAHHDSAPDDERPRLRNELAARHAQI
                     AAWAEHGPDRFRDRAELTGA"
BASE COUNT          403 a         1052 c         1114 g          407 t
ORIGIN      
        1 gcgccggtga gctcggcgcg gtcgcggaag cggtcggggc cgtgctccgc ccacgccgcg
       61 atctgggcgt ggcgggctgc gagctcgttg cgcagtcgcg gccgctcgtc gtccggcgcg
      121 ctgtcgtggt gggcggcgca cgcgagcgcg gcgtagaaca cgtgctccgc gccctcgggg
      181 aacgacggga tcttccacag cagcggccgg gccctggccg cggccgccat cgcgccggcg
      241 tgagcgccgg cgagaaaccg cgcctgcacc ttgtggatcc agtaccagca cgcggggaat
      301 ccgggttcgg cctcgaggcg ctgctcgaac cgatcctcgt cgaacgcctc gtcgtcgaac
      361 gagccgagct ctcgcgtcat cccgcggagc gaccggatca gctgccggtt cgtcgtgacg
      421 acgtcgacca tcatgccgaa cttgagcttg tgcatgaacg cgagcgcggc gtcggcctcg
      481 tccaggatcg ccgcgagcgg atcgcccgcc aggcaggaca gcgtgaccgg ggcgttgcag
      541 gcgtacgccg cgtacatcag atcgccggtc tccatcgcgg cgtcgaacgc gcgccgcgcc
      601 agctcgacgc cgtcgcgggg ttgcttcgtc cagggcagga tccggtagcc gagctcgacg
      661 tagatccgcg ccttccagcg cagcggctgg cgccgctcga ccagagcgaa cccgagctgg
      721 ccgaaccgga gcgccgcggc ggggttgccg aaccgcgtcc ggagcatcga gccgagcgcg
      781 gcgtaggccc agcacgaggc atcgctgtta ccgtgctcga ggctcaggtt caccgcccgg
      841 cagaccatca gcgagtgcag gttctcgtcg gcgaactgca cgggcggctg cgcgagcgtg
      901 agcagttcgg tcgtcgcgcg gcgttccgga tcggtcatcg gcggcaggtc ggcgaggtcc
      961 tcgatcgcgc gcgatccgag ctgctgccag atccggtcgt actcgcaccg gacctcgtcg
     1021 tcggtcgggt gcgccggcca cgcggtgccc gtgcgcccca ggtactcgag cgtcgcctcg
     1081 gccgcgagcg cgggccgatc gagcgtcgcg tagagcacga tccgcaccga cgtcaccgcc
     1141 gccaggtcga ggaggctgtc agcgcggcgg gcgagctcgg tgaggcgctg ctccgccgcg
     1201 tcgaacgcgc cggtcaggtg ctcgcactcg gcgcggtgga gctcgagcgc gaacgcgagc
     1261 tcggggcggt ggacctcggg cacctcggcg agcagcgcgg cgcccgcggt gaagtagccg
     1321 agcgccgagg cgtaggccgt cgaggcctgc gcgcgcgtgc cggcgagcag gtacagctcg
     1381 gcgagccgct cgcgctcctg cgccgactcg atcagcgccg cgccgcggtc gagctggctg
     1441 acgatctcga acaccttccc ggcgagctcc tccggtggcg tgctcgcgag cagccggcgg
     1501 ccgatcgcga ggtggatccg cgcgcgctcc ggctcgggca cgagcgcgta ggcggcctcg
     1561 tgcacgcggt cgtggggaaa gcgataggtc cgctgctcgc cctggacggt ctcgagcagc
     1621 aggtgctgct cgagcgcggg ggacagcgcc ggcgcgacct cgcgctcgcc ggcgatcgcc
     1681 agcgtggcgg cgtcgaccgt gctgccgagg cacgccgcga gctggagcgc ggtctgggtc
     1741 tcgcccggca gcgcgtacag ccggccgcgc accagctcgg cgatgttgtc ggtgtagccc
     1801 tcggcgcgga tccgcgccgc atcccattgc cagcggtacg cgtcgcgatc gaaccagatc
     1861 aggcgcttgt ggtgcagcgc ggtcaggaac tggatcgcga agaacgggtt gccgccggtc
     1921 ttgtcccgga ccaggccggt gagcggcgcc gcctcggcgg gtgaggtgtg gaccgtgtcg
     1981 gcgaccagcc ggccgagatc gtgctcggac agcggcccga gcacgaggtc gcggatccgc
     2041 gcggcgctcc gccgcgccgc ctcgagcgct cgcaccatcg ggtgcgccgg atcgacctcg
     2101 ttgtcgcgat aggcgccgat gatcagcaga tggcgattgt cgccgtcggt caggaggttg
     2161 gtgatcaggg cgaggctcgc gctgtccgcc cactgcatgt cgtcgatgaa catcgcgagc
     2221 gggtgatcgg gcgtcgcgca cgccgcgaac agcctgccga acaccacgcg gagccgggtc
     2281 tccgactcgg tcaggggcag ctcgggcacc gccggttgcg gtccgacgat caacccgagc
     2341 tgcgggacca gatcgacgat cagctggcag ttcgacccga gcgcctgggt gaatcgctgc
     2401 cgccaccgcg cgatccggag ctcgtcctcg gccaggatgt cgagcgcgag ctcgcgcagc
     2461 gcctggctga tcgtgaagta cgggatgtcg cgcttgtact gctcgaactt gcccgcgatc
     2521 agccggccgc gccgctggac gaccgtgcgc agcagctcgc gcaccaccga tgacttgccg
     2581 atgcccgcgt agcccgacac caggaccagc tccggcgtgc cagatccggc ggcgcgctcg
     2641 aacgcgtcgc ccagcgccgc gcactcggcg tcgcggccgt acagcttctg cgggatccgg
     2701 aactccgcgg agacgtcgcg ctcgccgagc gggaacgacg cgagcgcgcc gtgctccgtc
     2761 cagtcgcgca ggcagcgctc cagatcgtgg tggaggccga gcgcgctctg gtagcgatgg
     2821 tcgggcagct tcgccagcag cttgccgacg atgtcgatca gcgggcgcgg caccgacggt
     2881 cgtacctggt cgaacggccg cggctggcgc gcgacgtggc agtgcaccca gcccaccgcg
     2941 tcctcggcct cgaacggcaa ccggccggcg agcagc
//
LOCUS       DASVOR010000618         2036 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_181425,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000618 DASVOR010000000
VERSION     DASVOR010000618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181425"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VF516_12240"
     CDS             <1..969
                     /locus_tag="VF516_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108490.1"
                     /translation="LYNIAQAYRQTHDCKNALFFYKRFLALKANDTAKPLSAKTRKAV
                     EDFVAEADTCVKQQTQLSEKPPTQISQKSGGTNVPPPDGEAGDKGRPPADKGRPPTDK
                     GRPPGETARQEPPPPPRKEVATAQKPPSEPDSDPEDTGVTKPAIGGQPRMISARLIGG
                     GSKVSAGSISVPVQATFGVVGGHPFPINPKLTVEAGAAFTFTPTPYQGAMSGGKTGYL
                     VGAMANGGATYEVMPKLGIRGDLGLGALLFGGIDQSPFTNNAPTTGALTMFHARAAVS
                     ADYAITPNLVLTAMPGAFTYSPAAQGIVGGGAIWSYDFMLGIGYRR"
     gene            982..1668
                     /locus_tag="VF516_12245"
     CDS             982..1668
                     /locus_tag="VF516_12245"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEX8108491.1"
                     /translation="MKKCPFCAEEIQDEAIKCRFCSSFLAAAPGQPAADAGPAPGHAG
                     PPPAPFVREAGRDEAIVVRKMIYEGVPSWRTYFRHYVLSLLATAAVIAGLLVLGGKRS
                     GRPASLEIEALYVVVPLAAMGVFWFVLNLYRRSIKFRVTSTAIETERGFLSRRIDVLQ
                     LWRCRDVRFKQRLSDRILGIAHVEVFAQDATTPHLEIVGMPASRELFEQLRDSIEIQR
                     QAKNVYGVVS"
     gene            complement(1669..>2036)
                     /locus_tag="VF516_12250"
     CDS             complement(1669..>2036)
                     /locus_tag="VF516_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HEX8108492.1"
                     /translation="GLSLSGGEPLQQPEAARSLLDAARALGLSTLAFSGYTLDEIRGL
                     PGGPDVLARLDVLIDGRYVARDRLATGLRGSANQRIQLLTDRYALADVEATPVAEIRI
                     GPTGDVVLTGVDPLRLRQR"
BASE COUNT          310 a          716 c          747 g          263 t
ORIGIN      
        1 ctgtacaaca tcgcacaggc gtaccggcag acccacgact gcaagaacgc gctgttcttc
       61 tacaagcgct tcctcgcgct caaggccaac gacacggcca agccgctttc ggccaagacc
      121 cgcaaggcgg tcgaggactt cgtcgccgag gcggatacct gcgtcaagca gcagacccag
      181 ctcagcgaga agccgccgac ccagatcagc cagaagtcgg gggggaccaa tgtgccgccg
      241 cccgacggcg aggccggcga caaggggcgc ccccccgccg acaagggtcg cccccccacc
      301 gacaagggtc gcccccccgg cgagaccgcc cgccaggagc cgccgccgcc gccacgcaag
      361 gaggtcgcga ccgcgcagaa gccgccttcc gagccggaca gcgacccgga ggacaccggc
      421 gtgaccaagc cggcgatcgg cgggcagccg cggatgatct cggcgcggct gatcggcggc
      481 ggtagcaagg tcagcgccgg gagcatctcg gtcccggtcc aggcgacgtt cggcgtggtc
      541 ggcggccacc cgttcccgat caatcccaag ctgacggtcg aggccggcgc cgcgttcacc
      601 ttcacgccga cgccgtacca gggcgcgatg agcgggggca agaccggcta cctggtgggc
      661 gcgatggcca acggcggcgc gacctacgag gtgatgccga agctcgggat ccggggcgac
      721 ctcggcctgg gcgcgctgct gttcggggga atcgaccaga gcccgttcac caacaacgcg
      781 ccgaccaccg gcgcgctgac catgttccac gcccgcgcgg cggtgtcggc ggactacgcg
      841 atcacgccga acctcgtgct gaccgcgatg ccgggcgcgt tcacctacag cccggcggcc
      901 cagggcatcg tcggcggcgg agcgatctgg tcgtatgact tcatgctggg catcggctac
      961 cgccggtagc ccggagcggc gatgaagaag tgtccattct gcgccgagga gatccaggac
     1021 gaggcgatca agtgccggtt ctgtagctcg ttcctcgccg cggcgccggg gcagccggcc
     1081 gccgacgccg gtcccgcgcc ggggcatgcc gggccgccgc ccgcgccgtt cgtgcgcgag
     1141 gccggccgcg acgaggcgat cgtcgtgcgc aagatgatct acgagggcgt gccgtcgtgg
     1201 cggacgtact tccggcacta cgtgctgtcg ctgctcgcga ccgcggcggt gatcgccggc
     1261 ctgctggtgc tcggcggcaa gcggtccggc cggccggcgt cgctggagat cgaggcgctg
     1321 tacgtggtcg tgccgctcgc ggcgatgggg gtgttctggt tcgtcctcaa cctgtaccgc
     1381 cggtcgatca agttccgggt gaccagcacg gcgatcgaga ccgagcgggg cttcctgtcg
     1441 cggcggatcg acgtgctgca gctgtggcgc tgccgcgacg tccgcttcaa gcagcggctc
     1501 agcgaccgga tcctcggcat cgcccacgtc gaggtgttcg cccaggacgc gacgacgccg
     1561 cacctggaga tcgtcgggat gccggcgtcg cgcgagctgt tcgagcagct gcgcgactcg
     1621 atcgagatcc agcgccaggc caagaacgtc tacggcgtgg tcagctgact agcgctgcct
     1681 gagccgcagc gggtcgacgc cggtgagcac gacgtcgccg gtcggcccga tccggatctc
     1741 ggcgaccggc gtggcctcga cgtcggcgag cgcgtagcga tcggtgagca gctggatccg
     1801 ctggttggcc gagccgcgca ggccggtggc gagccgatcg cgcgcgacgt agcggccgtc
     1861 gatcaggacg tcgagccgcg ccagcacgtc ggggccgccg ggcaggccgc ggatctcgtc
     1921 gagcgtgtag ccggagaacg ccagggtcga caggccgagg gcccgggcgg cgtcgagcag
     1981 gctgcgcgcg gcctcgggct gctgcagcgg ctcaccgccg gacagcgaca ggccct
//
LOCUS       DASVOR010000619         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_239533,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000619 DASVOR010000000
VERSION     DASVOR010000619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239533"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(484..1368)
                     /locus_tag="VF516_12255"
     CDS             complement(484..1368)
                     /locus_tag="VF516_12255"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEX8108493.1"
                     /translation="MAIGLGIVVLALVAYLGVLFALTPNVSHLRDAPPTQTSFMRVRA
                     AERTRAAADRVEWIDLGQVSPYLVCAIIKAEDRGFFRHSGFEWGQLRKALWGHVTGGS
                     GIGGSTITQQLARNLYLSPARTLHRKLREALITRRLESSLDKRRILELYLNVVEWGDG
                     VWGVTQAARAYFGRSPAELGLFEAVFLASLLPAPRAGLHGRDIDRAATEERRVLHQLV
                     VSGLIDPAAFSDARARVALLVSSLSRGEPLAQALQAAATPAIRARPLELSEVLANECG
                     LAHELTAGARARRLVKPR"
     gene            complement(1392..>2646)
                     /locus_tag="VF516_12260"
     CDS             complement(1392..>2646)
                     /locus_tag="VF516_12260"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8108494.1"
                     /translation="MVSAHAFRLRDRRMPAVALAAMAIATVVFSRVVLRSDHQLAALS
                     AGWPLLVVAGTLAAIAVHWIRAVVRGRFHALSSVELALASCVMWACIATLAMPRAPLW
                     TYAVLPYLALGGVFVAARRLVDHTAVPWVLALIGALVAASVLLDAAGLAVVVSRRPGG
                     LLGNRNFAGEYLALALPAALAVFTRSRRWWWILVAFGGTLALTRCRTAWLAASVAVVA
                     LVALSPPPLRSRRVLGAALVLAGVLLASVVPTRLAWNEAHPFTSTLSRAVDLEDGSGQ
                     LRVRQYVATLGVLDSRGAWLTGLGPGAWQAAVRAQDRAIAKNRVPHSDYLRVLSDAGA
                     PALGGLCILLVVAALAAWQLRREHPELVVFLGVLALEALADAPLFRPEVIVATGAVLA
                     TLHARLLMARGSSCWLEPAPAGHGR"
BASE COUNT          467 a          922 c          950 g          307 t
ORIGIN      
        1 cttggcgacc gcatcgaagt ccgcgtcctt gatgtcgtcg taggtcttct tgccgccctc
       61 gacgtgctgc tccgtgctcg cgatcgcgac cgtgcggtca cggtcgcacg cgccgccgat
      121 cgcgagcaaa ccgatcaaag ctgcaaacaa aaatttcttc attgcaaaca cctccgcgtg
      181 ccgagcctgc aaccggcaga ccggagcgac ctcgcgacga gcggacgtgt tacgcagcgc
      241 gcagtgcagg cgcgcgcctg cagtgacggc gcgagcacgc agcagcggcg tcgagatcgc
      301 gagcgcgcgg atccgaagcg caacgagcga cgcgccgact gtattgccgg ctgcgggcag
      361 cagccgcgac atcgggccgc gcgaggacgt tcgcgtggca gtgggcttgc agcaggtacg
      421 gcgggaagga agaaccatga acggttcatg agcgatctac gcgacgagtg aaggacgcgc
      481 gggtcagcgt ggtttcacga ggcgtcgcgc acgggccccg gcggtgagct cgtgggcgag
      541 gccgcattcg ttcgcgagca cctccgagag ctcgagcggt cgcgcacgga tcgccggcgt
      601 cgctgcggcc tggagcgcct gcgccagcgg ctcgccgcgc gaaagcgagc tcaccagcag
      661 cgcgacgcgt gcccgcgcgt cggagaatgc tgcgggatcg atcagcccgg agacgaccag
      721 ctgatgaagt acgcgcctct cctcggtggc agcgcgatcg atgtccctgc cgtggagacc
      781 cgcgcgcggc gccggcaaca agctcgcgag gaacacggcc tcgaacaggc cgagctcggc
      841 gggactccgg ccaaagtagg cccgtgcggc ctgcgtcacg ccccacacgc catcgcccca
      901 ttcgacgacg ttgagataga gctcgaggat gcggcgcttg tcgagcgagc tctccagccg
      961 acgcgtgatc agtgcctcgc gaagcttgcg gtggagcgtg cgcgcggggc tgaggtagag
     1021 gttgcgcgcg agctgctgcg tgatcgtgct gccgccgatg cccgagccgc cggtgacgtg
     1081 gccccacaat gccttgcgga gctggcccca ctcgaagccg gagtggcgaa agaagccgcg
     1141 atcctccgcc ttgatgatcg cgcacaccag gtacggcgac acctggccga ggtcgatcca
     1201 ctcgacgcga tctgcggctg cgcgcgtgcg ctccgcggcg cgcacgcgca tgaagctggt
     1261 ctgcgtcggt ggcgcgtcgc gcaggtgact cacgttcgga gtcagggcga acagcacgcc
     1321 cagataggcc acgagcgcga gcacgacgat gcccaggccg atcgccacgc gacggaaccg
     1381 gcggcgccct ctcatcggcc gtgcccggcg ggtgcaggct cgagccagca gctgctgccg
     1441 cgagccatca gcagtcgtgc gtgcagcgtc gccagcacgg ctccggtcgc cacgatgact
     1501 tccggacgga acaggggagc gtcggcgagc gcctcgagcg cgaggacgcc gaggaagacc
     1561 acgagctcgg ggtgctcgcg gcgcagctgc caggccgcca gcgcggccac caccagcagg
     1621 atgcatagcc cgccgagcgc cggcgcacct gcatcggaga gcacgcggag gtaatcgctg
     1681 tgcggaacgc ggttcttggc gatggctcgg tcttgcgcgc gaaccgccgc ttgccacgct
     1741 cccggaccga ggccggtcag ccatgcgccg cgggagtcga gcacgccaag cgtcgcgacg
     1801 tactggcgga cacgtagctg tccagagccg tcctccagat cgacggcgcg cgacagcgtc
     1861 gaagtgaacg ggtgcgcttc gttccacgcg aggcgcgtcg gcaccacgga agcgagcagg
     1921 acaccggcga ggaccagcgc ggcgccgagc acgcgacgac tccgcagcgg cggcggcgac
     1981 agcgcgacca gcgcgaccac ggcgacggac gccgcgagcc acgccgtccg gcaccgggtc
     2041 agcgcgagcg taccgccaaa cgcgacgaga atccaccacc agcgtcgcga gcgcgtgaac
     2101 accgcgagcg cggcgggaag tgccagcgcg aggtattcgc ccgcgaagtt gcgattgccg
     2161 agcaggccac cgggccgccg cgagaccacg acggcaagcc cggcggcgtc gaggagcacg
     2221 ctcgcggcga ccagcgcgcc gatcagcgcg agcacccacg gcaccgccgt gtgatcgacc
     2281 aggcgccgcg cggcgacgaa cacaccgccg agcgcgagat acggcagcac ggcatacgtc
     2341 cacaggggtg ctcgcggcat cgccagcgtg gcgatgcatg cccacatgac gcagctggcc
     2401 agcgcgagct ccaccgagga cagcgcgtgg aaccggccgc gcaccaccgc ccgtatccag
     2461 tgcacggcga tcgccgcgag ggtgccggcg acgacgagga gcggccagcc cgccgagagt
     2521 gcagcgagct gatgatcgga ccgaaggacg actctcgaga acacgaccgt cgcgatcgcc
     2581 atcgccgcca gtgccaccgc gggcatgcgc cggtcgcgca ggcgaaacgc gtgcgcggat
     2641 accatg
//
LOCUS       DASVOR010000620         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_444037,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000620 DASVOR010000000
VERSION     DASVOR010000620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444037"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF516_12265"
     CDS             complement(<1..192)
                     /locus_tag="VF516_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108495.1"
                     /translation="MTPRGHALVAFVVLAALAALETCRLGNPDPAPAGAPPEQFSASR
                     AITTLRGVLGEASLDQPHPL"
     gene            complement(214..1335)
                     /locus_tag="VF516_12270"
     CDS             complement(214..1335)
                     /locus_tag="VF516_12270"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8108496.1"
                     /translation="MTIQARRTRKVDKLARIYDDEIAPCWGARFARLLLRNLLVPERC
                     QVLDVSCGTGDPAIEILRRMSDGSRLIAIDESSAMLDAARRKVAALGPLAKKVYFRTE
                     SAVPKLSFADEVYDLVVCNLGVGDMPSVEIALRDFARVAKPGGEVRCTLPLAGTFEEF
                     HDLYREVLVKHDLHDALDRLDLHIARYPTYDHAEACLAAAGLTGGIEVEEFSLLFRSA
                     REFFFAPVIEYGPLADWKVVAGGGQEMQDVFWYIKEAIDAYFGDRPFHVTVKAALVAG
                     KKPQRPVIDPVDSLTADSITTPVPLVDTDQDSPFDHIETGLPWPPRDIAGGSVVEDSG
                     DAAIEELASDEYELDEDARAEQELDALIDGRKRPQHLID"
     gene            complement(1469..>1777)
                     /locus_tag="VF516_12275"
     CDS             complement(1469..>1777)
                     /locus_tag="VF516_12275"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX8108497.1"
                     /translation="RDNALSKARFEFRWQDQFNLSLDPDTAKDFHDETLPAPAAKGAH
                     FCSMCGPKFCSMKITEEVRQMAAGGDHAAPDVEAGLAAKAAEFLEGGQRIYAGGDDPH
                     "
BASE COUNT          261 a          625 c          631 g          260 t
ORIGIN      
        1 gagcgggtgc ggctggtcga gcgaggcctc tccaagaaca ccgcgcaggg tcgtgatcgc
       61 gcggctcgcc gagaactgct cgggcggcgc gccggccggc gcgggatcgg ggttgccgag
      121 ccggcaggtc tcgagcgcgg cgagcgccgc gagcacgacg aaggcgacca gggcgtgtcc
      181 gcgaggcgtc atcgccgcat tgtacgcggg cggtcagtcg atcaggtgct gcggccgctt
      241 gcgaccgtcg atcagcgcat cgagctcctg ctcggcgcgg gcgtcctcgt cgagctcgta
      301 ctcgtcagac gcgagctcct cgatcgcggc gtcgcccgag tcctcgacca ccgagccgcc
      361 ggcgatgtcg cgcggcggcc acggcagccc ggtctcgatg tgatcgaacg gcgagtcctg
      421 atcggtgtcg accagcggga ccggcgtggt gatgctgtcg gcggtgagcg agtcgacggg
      481 gtcgatgacc gggcgctgcg gctttttgcc cgccaccagg gcggccttga cggtgacgtg
      541 gaacggccgg tcgccgaagt aggcgtcgat cgcctccttg atgtaccaga acacgtcctg
      601 catctcctgg ccgccgccgg cgaccacctt ccagtcggcg agcgggccgt actcgatcac
      661 cggcgcgaag aagaactcgc gcgcgctgcg gaacagcagc gagaactcct cgacctcgat
      721 gccgccggtc aggccggccg cggccaggca ggcctcggcg tggtcgtagg tcggatagcg
      781 ggcgatgtgg aggtcgagcc ggtcgagcgc gtcgtgcagg tcgtgcttga ccaggacctc
      841 gcggtacagg tcgtggaact cctcgaacgt gcccgcgagc ggcagcgtgc agcggacctc
      901 gccgcccggc ttggcgaccc gcgcgaagtc gcgcagcgcg atctcgacgc tcggcatgtc
      961 gccgacgccg aggttgcaca ccacgagatc gtagacctcg tcggcgaacg acagcttggg
     1021 caccgcgctc tcggtgcgga agtacacctt cttggccagc gggccgagcg cggcgacctt
     1081 gcggcgcgcc gcgtcgagca tcgcgctcga ctcgtcgatc gcgatcagcc gcgagccatc
     1141 gctcatccgg cgcaggatct cgatcgccgg atcgccggtg ccgcacgaca cgtcgagcac
     1201 ctggcagcgc tcggggacca gcaggttgcg cagcagcagc cgggcgaacc gcgctcccca
     1261 gcacggcgcg atctcgtcat cgtagatacg cgcgagctta tcgaccttgc gggtacgccg
     1321 ggcctggatc gtcacgacag agcatcaccg acggggtgac gcccgcgaga ataccaagca
     1381 cctcccagcc ggtccatgca gcgttccacc cagctgccag ggtgccagct gccaggtgcc
     1441 gcaccgcgcc acaccgccac cgcccgcgtc agtgcggatc gtcgccgccg gcgtagatcc
     1501 gctggccgcc ctcgagaaac tcggcggcct tggccgccag gccggcctcg acgtccggcg
     1561 ctgcgtggtc gccccctgcg gccatctgcc gcacctcctc ggtgatcttc atcgagcaga
     1621 acttcggccc gcacatcgag cagaagtgcg cgcccttggc cgccggggcc ggcagggtct
     1681 cgtcgtggaa gtccttcgcg gtgtcgggat cgagcgacag gttgaactgg tcctgccagc
     1741 ggaactcgaa ccgcgccttg ctgagcgcgt tgtcgcg
//
LOCUS       DASVOR010000621         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_181515,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000621 DASVOR010000000
VERSION     DASVOR010000621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181515"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VF516_12280"
     CDS             complement(<1..356)
                     /locus_tag="VF516_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108498.1"
                     /translation="MTWRDGVHLTGTPIWCDARRRRDVCFLSSADRVGRTGHGQLIGT
                     AITLALVDATGPGHLAVPLRQRFTLGTVRLELIPSGRGFGAAALHADVAGKTVLYAGA
                     VRTSTGGVGDAAELRT"
     gene            complement(494..1126)
                     /locus_tag="VF516_12285"
     CDS             complement(494..1126)
                     /locus_tag="VF516_12285"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8108499.1"
                     /translation="MAAIRLELRLSHQSDWIKIFDPRDWTIFVPTAEAVGQSTAVRID
                     LDVGGWLVALRGTVVGRRDEPTGVVVALGGTERDKINYLNGFVRGGLLNLREKRRLPI
                     RLAVTYGAVEGPASTFTKDINEEGVFLFTDRPLPETSQVHMLLSLPDRAQPLSLIGKV
                     SHTILAADDEPPGMGIVFDLDDAARNQLVEIVRELEAQLLAGKLPTGAVE"
     gene            complement(1226..>1572)
                     /locus_tag="VF516_12290"
     CDS             complement(1226..>1572)
                     /locus_tag="VF516_12290"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8108500.1"
                     /translation="EVRSAATAPAPAPGDAIAISRSDLDGALADFARLTAGMRGSFSA
                     NGVVIDGVGDGTIFQRAGLRAGDVVTSVDGVRLRTLDDAANLYARVSTARAITAQVVR
                     AGRPITLHVVIQ"
BASE COUNT          219 a          549 c          588 g          216 t
ORIGIN      
        1 caggtccgca gctcggcggc atccccgacg ccgccggtgc tggtccgcac cgcaccggcg
       61 tacagcacgg tcttgccggc gacgtcggcg tggagcgcgg cggcgccgaa cccgcggccc
      121 gacgggatca gctcgagccg caccgtgccg agcgtgaagc gctggcgcag cggcacggcg
      181 aggtgaccgg ggccggtcgc gtcgaccagc gcgagcgtga tcgcggtgcc gatcagctgg
      241 ccgtggccgg tgcgcccgac ccgatcggcc gacgacagga agcacacgtc gcggcgccgg
      301 cgcgcgtcgc accagatcgg cgtcccggtg aggtggacgc catcgcgcca cgtcaccgga
      361 gcgttcgggg tggttcgcgg acgacgcacc accgcacacg gtgccactca tcgaccgatc
      421 gagcaatgat ctggctgacc tacacgtgcg tgatggagat ctcgcccact cgaccgtgcc
      481 ggttcgccct gcctcattcc acggcgccgg tcggcagctt gccggcgagg agctgggcct
      541 cgagctcgcg gacgatctcg acgagctggt tgcgcgccgc gtcgtcgagg tcgaacacga
      601 tgcccatgcc gggcggctcg tcgtccgcgg cgaggatggt gtgcgagacc ttgccgatca
      661 gcgacagcgg ctgggcgcgg tcgggcaggc tgagcagcat gtggacctgc gaggtctcgg
      721 gcagcgggcg gtcggtgaac aggaacacgc cctcctcgtt gatgtccttg gtgaaggtgc
      781 tcgccggccc ctcgacggcg ccgtaggtca ccgcgagccg gatcgggagc cggcgcttct
      841 cgcgcaggtt gagcagcccg ccgcgcacga agccgttcag gtagttgatc ttgtcgcgct
      901 cggtgccgcc gagcgccacg accacgccgg tgggctcgtc gcggcggccg accacggtgc
      961 cgcgcagcgc gaccagccag ccgccgacgt cgaggtcgat ccgcacggcc gtgctctgcc
     1021 cgacggcctc ggcggtcggc acgaagatcg tccagtcgcg cggatcgaag atcttgatcc
     1081 agtcgctctg atgggacagc cgaagctcca gccggatcgc ggccaccggg ccatcgtaaa
     1141 ccactccgat cgcagatcca ccgccgggcc ccggcgtgta cgcgtgtact ggcgtgtagt
     1201 gatgcgacgc ctgcgacgcc tgcgactact ggatcacgac gtggagcgtg atcggtcttc
     1261 cggcgcggac gacctgggcg gtgatcgccc tggcggtcga cacgcgggcg tacaggttgg
     1321 cggcgtcgtc gagcgtgcgc agccggacgc cgtcgacgct ggtgacgacg tcgccggcgc
     1381 gcaggcccgc gcgctggaag atcgtgccgt cgccgacccc gtcgatcacg acgccgttcg
     1441 ccgagaagct gccgcgcatg ccggcggtga gccgcgcgaa gtcggcgagc gcgccgtcga
     1501 gatcgctgcg cgagatcgcg atggcgtcgc cgggggcggg cgcgggtgca gtcgccgcgg
     1561 agcgcacctc gg
//
LOCUS       DASVOR010000622         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_34996,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000622 DASVOR010000000
VERSION     DASVOR010000622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34996"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2360)
                     /locus_tag="VF516_12295"
     CDS             complement(<1..2360)
                     /locus_tag="VF516_12295"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HEX8108501.1"
                     /translation="MVDWAGKLAPAATGQIHVELAHLEADYGYGYDEASGEGRLDRWL
                     RRVPEGKRDVQALGGKFSFDVTPGEASAGFQIRVTAGKATTELVVDGEYPYEYYGYGE
                     DNHADQMPRPARPTQLKVVLPKDIKTGEAVTASIRSPYRGKVLWTVETDRLVTSAWKD
                     VAAGDASWSFTLDRFAPNVYVSAFVIKDPHLESKDAFLPDRAFGVGGAHVVPVEATQP
                     LQITAPKEVRSSSRLTVALDAGKIDGPAFATVAVVDEGILSLTGFASPDPLAQLFARR
                     ALGVETYETIGWTMLHQPAGASSKTGGGEEGFDEASEYGATLGGRVQPVKPVALFSGI
                     VAVGASGKIEIPFQVPQYRGELRVMAVVATPGRVGRAEAHVTVKDPLVLQVTFPRFIT
                     QNDEVQIPVFMTNLSGGPLEVTVALSSESLAIPGLAAPRAATPPLRFTGKDTGTVKIA
                     NGFAETAVFRAKAQIAAGGARLRVVARARGPAGAFEVKDEVDVPFLPAGPRERAIQKI
                     RLDAGALDLAARATVLKNWVPTSETTTFWMTTNPYGESFDHLGYLIHYPYGCIEQTTS
                     STRPLLYVASLVEQVDPQLAEAKIEDMVLAGINRVFTMQTPAGGFGYWPGATEPLEWA
                     TAYATDMLLDARKAGYAVPDDRLKEVMAWIEGRVAQYERGEKIRREPWNHYDEQSEAY
                     LHYVLARAGKGRKARILALIEHMPAGARGEQAEDRTLLEAALFLAGDRRFDKELRAVD
                     ASPIADDRLKEVMAWIEGRVAQYERGEKIRHEPWNHYDEQSEVYLH"
BASE COUNT          305 a          882 c          817 g          370 t
ORIGIN      
        1 tagtgcaggt agacctcgga ctgttcatcg taatggttcc acggctcgtg ccggatcttc
       61 tcgccgcgct cgtactgcgc gacccggccc tcgatccacg ccatcacctc cttgagccga
      121 tcgtcggcga tcggcgacgc gtcgaccgcc cgcagctcct tgtcgaaccg gcggtcgccg
      181 gcgaggaaca gcgcggcctc gagcagggtg cggtcctcgg cctgctcgcc gcgcgccccg
      241 gccggcatgt gctcgatcag cgcgaggatc cgcgccttgc ggcccttgcc ggcgcgcgcc
      301 agcacgtagt gcaggtacgc ctcggactgc tcgtcgtagt ggttccacgg ctcgcgccgg
      361 atcttctcgc cgcgctcgta ctgcgcgacc cggccctcga tccacgccat cacctccttg
      421 agccgatcgt cgggcacggc gtagccggcc ttcctggcgt cgagcagcat gtcggtcgcg
      481 tacgccgtcg cccactccag cggctcggtc gcgcccggcc agtagccgaa cccgcccgcc
      541 ggcgtctgca tcgtgaacac gcggttgatt ccggcgagca ccatgtcctc gatcttggcc
      601 tcggcgagct gcgggtcgac ctgctcgacc aggctcgcga cgtagagcag cggccgggtc
      661 gacgacgtgg tctgctcgat gcagccgtag gggtaatgga tgagatagcc gaggtgatcg
      721 aacgactcgc cgtacgggtt ggtcgtcatc cagaacgtcg tggtctcgct ggtcggcacc
      781 cagttcttga gcacggtcgc cctggccgcg agatcgagcg cgccggcgtc gagccggatc
      841 ttctggatcg cgcgctcgcg cggcccggcg ggcaggaacg gcacgtcgac ctcgtccttg
      901 acctcgaacg cgccggccgg gccccgagcg cgcgcgacga cccgcagccg ggcgccgccg
      961 gccgcgatct gcgccttggc ccggaacacc gcggtctcgg cgaagccgtt ggcgatcttc
     1021 accgtgccgg tgtccttgcc ggtgaagcgg agcggcggcg tggcggccct gggcgccgcg
     1081 agccccggga tcgcgaggct ctcgctcgac agcgcgaccg tcacctcgag cgggccgccc
     1141 gacaggttgg tcatgaacac cgggatctgg acctcgtcgt tctgggtgat gaaccgcggg
     1201 aacgtgacct ggaggaccag cgggtccttg accgtgacgt gcgcctcggc gcggccgacc
     1261 cgccccgggg tcgcgaccac cgccatcacc cgcagctcgc cacggtactg cggcacctgg
     1321 aacgggatct cgatcttgcc actggcgccg accgcgacga tgccggagaa cagcgcgacc
     1381 ggcttgaccg gctggacccg gccgccgagc gtggccccat attcgctggc ctcgtcgaag
     1441 ccctcctcgc cgccgccggt cttcgacgac gcgccggccg gctggtgcag catcgtccag
     1501 ccgatcgtct cgtaggtctc gaccccgagc gcgcgccgcg cgaacagctg ggccagcgga
     1561 tcggggctcg cgaacccggt gagcgacagg atgccctcgt cgaccaccgc gacggtcgcg
     1621 aacgccggcc cgtcgatctt gccggcgtcg agcgcgacgg tcagccggct cgacgaccgc
     1681 acctccttcg gcgcggtgat ctgcagcggc tgggtcgcct cgaccggcac gacgtgtgcc
     1741 ccgccgacgc cgaacgcgcg atcgggcagg aacgcgtcct tggactccag gtgcgggtcc
     1801 ttgatcacga acgcgctgac gtagacgttg ggcgcgaacc ggtcgagcgt gaagctccag
     1861 ctcgcgtcgc ccgccgcgac gtccttccac gccgacgtga ccaggcggtc ggtctcgacc
     1921 gtccacagca ccttgccgcg gtacggcgac cggatgctcg cggtcaccgc ctcgccggtc
     1981 ttgatgtcct tcggcagcac gaccttgagc tgggtcggcc tggccgggcg cggcatctgg
     2041 tcggcgtggt tgtcctcgcc gtagccgtag tactcgtagg ggtactcgcc gtcgaccacc
     2101 agctcggtcg tcgccttgcc cgcggtgacc cggatctgga accccgccga cgcctcgccg
     2161 ggcgtcacgt cgaagctgaa cttgccgccc agggcctgga cgtcgcgctt gccctcgggg
     2221 acccggcgca gccagcggtc gagccggccc tcgccgctgg cctcgtcgta gccgtagccg
     2281 tagtcggcct cgaggtgggc gagctcgacg tggatctggc cggtcgccgc cggggcgagc
     2341 ttgccggccc agtcgaccac cgcgccctcg accg
//
LOCUS       DASVOR010000623         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_414471,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000623 DASVOR010000000
VERSION     DASVOR010000623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414471"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="VF516_12300"
     CDS             complement(<1..1519)
                     /locus_tag="VF516_12300"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108502.1"
                     /translation="MSYRLAGVLCALSACSLNVDYTGTLFACDPDGACPAHYVCQDMR
                     CVPTEPPPAACFAAIGAGDGHSCAIRKDGAAWCWGSNNFGQLGDGTTTDRTVPVAVSG
                     TKLPRLSAIAGGDEHTCALGSDGSVWCWGHNNVGQLGNGSTSDFHAPVAVSNLSDATA
                     IASGAAHTCAIRADHSVVCWGHNDDGQLGNGASNDQSAPSPIDSLHGVTAIAAGGDAT
                     CAVDGNHQLWCWGDNAHGQLGDGTKMSRPMPTPITLPDIAGTAVGDGFSCALSSPSSG
                     GKVTCFGLNGDGQLGATVDPNAEHLIGVPVEITGVAVSFVARPNFACLTDDHQRVWCW
                     GANGNFQLADATSNDRFVPVLAGYGAAAAVVAGGAHTCALSSQGGVTCSGYNGHGQLG
                     NSRRTSQGTPHVVAGVQNAVSVTAGVFHTCAMLRDGTATCWGTNDLGELGDGTWTSRA
                     HPGPVSSLTGAQMLASGFVHSCALAGGAAVCWGFNENGQLGDTTTYTRGYPRPVSG"
     gene            complement(1519..2550)
                     /locus_tag="VF516_12305"
     CDS             complement(1519..2550)
                     /locus_tag="VF516_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108503.1"
                     /translation="MIACLRRSRGLRRLQALPWIALVWLQLEAVAWADDAVAQARAAV
                     AQSDYAAALPLLIAALDTGGRGREELAEIYQLTGVVEAALGDTRAATQAFLHVLVLSP
                     AATLPDGTSPKIKRPFDEAARYVASYGTLQIKFDWPNQIGPAAVVVANDPLDMVAMVR
                     VVTEPGGRQDLEVLSERIEFATPGDAALAYHVNVTVLDVHGNTLAGVVYMRDVRPREP
                     YVPPRSTIVVHPRPRPVYLRWWPYAGAGVATLAATGYVARAARSASHDHDGIIASSAQ
                     HHYGDARAVEDRARRNVLLTNIGLGVSGALAVVAGALYVTRPRDRVEPLGGIAPLPGG
                     GQLVLGGRF"
     gene            complement(2626..>2890)
                     /locus_tag="VF516_12310"
     CDS             complement(2626..>2890)
                     /locus_tag="VF516_12310"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8108504.1"
                     /translation="KRVTGAPRPPAAASKPSGPPGFITIDSSPVYAAIYIDGKSYGET
                     PLVRVELSPGRHVVHAVSPSGATRDVRITIESGKVSPATRIAW"
BASE COUNT          381 a         1026 c         1092 g          391 t
ORIGIN      
        1 ggccggacac cgggcgcggg tagccacgcg tgtaggtcgt ggtgtcgccg agctggccgt
       61 tttcgttgaa gccccagcac acggcggcac cgccggccag cgcgcacgag tggacgaacc
      121 cgctggcgag catctgcgcg ccggtcagcg agctcaccgg cccgggatgc gcgcggctgg
      181 tccacgtgcc gtcgccgagc tcgccgaggt cgttggtgcc ccagcaggtc gccgtgccgt
      241 ccctgagcat cgcgcaggtg tggaacacgc cggcggtgac cgagaccgcg ttctggaccc
      301 cggcgaccac atgcggggtg ccctgcgagg tgcggcggga gttgccgagc tggccgtggc
      361 cgttgtagcc gctgcaggtg acgccgccct gggacgacag cgcgcaggtg tgggcgccgc
      421 cggcgaccac cgccgcggcg gcgccgtagc cggcgagcac cgggacgaag cgatcgttac
      481 tggtagcgtc ggcgagctgg aagttgccgt tcgcgcccca gcaccacacg cgctggtggt
      541 cgtcggtcag gcacgcgaag ttggggcgcg cgacgaacga gaccgcgacg ccggtgatct
      601 cgaccggcac gccgatcagg tgctcggcgt tggggtcgac ggtggcgccg agctggccgt
      661 cgccgttgag cccgaagcag gtcaccttgc cgccgctcga cggcgagctc agcgcgcacg
      721 agaacccgtc gccgaccgcc gtgccggcga tgtcggggag cgtgatcggg gtcggcatcg
      781 ggcgcgacat cttggtgccg tcgccgagct ggccgtgggc gttgtcgccc cagcaccaca
      841 gctggtggtt gccgtcgacc gcgcaggtcg cgtcgccgcc ggcggcgatc gcggtgacgc
      901 cgtgcaggct gtcgatcggc gacggcgcgg actggtcgtt gctggcgccg ttgccgagct
      961 gaccgtcgtc gttgtgaccc cagcacacga cgctgtggtc ggcgcggatc gcgcaggtgt
     1021 gggccgcacc gctggcgatc gcggtcgcgt cgctcaggtt cgacaccgcg accggcgcgt
     1081 ggaagtcgct ggtcgagccg ttgccgagct ggccgacgtt gttgtggccc cagcaccaca
     1141 cgctgccgtc gctgccgagc gcgcaggtgt gctcgtcgcc gccggcgatc gcagagagcc
     1201 tgggcagctt ggtgccggac accgcgaccg gcacggtccg atcggtcgtg gtgccgtcgc
     1261 cgagctggcc gaagttgttg ctgccccagc accacgccgc gccgtccttg cggatcgcac
     1321 agctgtggcc gtcgccggcg ccgatcgccg cgaagcacgc cgcgggtggt ggctcggtcg
     1381 gcacgcaccg catgtcctgg cacacgtagt gcgccgggca cgcgccgtcg ggatcgcacg
     1441 cgaacagggt cccggtgtag tcgacgttga gcgagcacgc ggacagcgcg cagagcacac
     1501 ctgccagtcg ataggacatc agaacctgcc tcccaggacg agttgcccgc cgcccgggag
     1561 cggcgcgatg ccgcccaacg gctcgacacg atcgcgcggc cgggtcacgt acagcgcacc
     1621 ggccacgacc gcgagcgctc cggagacccc gaggccgatg ttggtgagca ggacgttgcg
     1681 ccgggcgcgg tcctcgaccg cccgcgcgtc gccgtagtgg tgctgcgcgc tgctggcgat
     1741 gatcccgtcg tggtcgtggc tcgccgaccg cgccgcgcgc gcgacgtagc cggtggcggc
     1801 gagcgtcgcg accccggcgc cggcgtaggg ccaccaccgc aggtagaccg gccgcgggcg
     1861 cgggtgcacc acgatcgtcg atcgcggcgg gacgtaaggt tccctgggcc ggacgtcgcg
     1921 catgtacacc accccggcca gcgtgttgcc gtggacatcg agcaccgtga cgttcacgtg
     1981 gtacgcgagg gcggcgtcgc ccggcgtcgc gaactcgatc cgctccgaga ggacctcgag
     2041 gtcctgccgg ccgccgggct cggtcaccac gcgcaccatc gcgaccatgt cgagcggatc
     2101 attcgccacg accaccgcgg ccggcccgat ctggttgggc cagtcgaact tgatctgcag
     2161 cgtcccgtag ctcgcgacgt agcgcgcggc ctcgtcgaac gggcgcttga tcttgggcga
     2221 cgtcccgtcc ggcagcgtcg ccgcgggcga cagcacgagc acgtggagga acgcctgggt
     2281 cgcggcccgg gtgtcgccca gcgcggcctc gaccaccccg gtgagctggt agatctccgc
     2341 cagctcctcg cggccgcggc cgccggtgtc gagcgcggcg atcagcaggg gcagcgccgc
     2401 ggcgtagtcc gactgggcga ccgcggcgcg ggcctgcgca accgcgtcgt cggcccacgc
     2461 gacggcctcg agctggagcc agacgagcgc gatccacggc agagcctgga ggcgccggag
     2521 gccccgcgag cgccggagac aggcgatcat ccgcatcaga ctatcactcg ccgcggcgcc
     2581 gggagtggcc gcgccggccg cctgcctcgt gatgccgatc accggctacc aggcgatgcg
     2641 ggtggcgggt gagaccttgc ctgactcgat cgtgatccgg acgtcgcgcg tggcgcccga
     2701 cggcgagacc gcatgcacca cgtgacggcc cggtgacagc tcgacccgga ccagcggcgt
     2761 ctcgccgtag ctcttgccgt cgatgtagat cgccgcgtag accggcgacg agtcgatcgt
     2821 gatgaacccc ggcggaccgc tcggcttgct cgcagcggcg ggcggccggg gcgcgccggt
     2881 gacgcgcttg
//
LOCUS       DASVOR010000624         2174 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_298283,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000624 DASVOR010000000
VERSION     DASVOR010000624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298283"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VF516_12315"
     CDS             <1..1481
                     /locus_tag="VF516_12315"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX8108505.1"
                     /translation="LRATAGRLGLIAIDEAHCISEWGHDFRPDYRRLGDAVRLLGPPR
                     IAAFTATATPEVRRDIAAQLAFASPALHVRGFDRPNLHYAVERVGGGDDKSSALVERI
                     RTRDGGVALVYAATRKNAEKYASALKLAGMRARVYHAGLDGEVRERAQDVFMAGKLDA
                     IVATNAFGMGVDKSDIRLVVHADIPRSPEAYYQEAGRGGRDGKPTRCVLLFNHSDIKL
                     QEFLIDASYPSAEVLRGVWKLLRDRPQLGRLTPYDDELENRLRNQLGGDVNGATMGAA
                     LRILERHGMLRRDDENLMAARPEPGVFLPLDVESLQRRADVERSKLRTMVEYAYYPRC
                     RRQFVLEYFGDQDWSDRDRRCGACDTCEAIAHGRPTAMQLSPSELAAIKNLLSLVGAL
                     HGRFGRMRVVKLAVGSDDDTRFDDLPERGCLRGWRERAALDLLRALEGVGLVEASRGE
                     YPTIALTRRGSQVAAGTLDAGTLDVQMPSPGKQARSKRSRKR"
     gene            complement(1492..1650)
                     /locus_tag="VF516_12320"
     CDS             complement(1492..1650)
                     /locus_tag="VF516_12320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108506.1"
                     /translation="MSPGDAFPRIFSILLRLAFTLALIGGISAAYRAEEMARNRQTPE
                     TAAAPDGE"
     gene            1982..>2174
                     /locus_tag="VF516_12325"
     CDS             1982..>2174
                     /locus_tag="VF516_12325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108507.1"
                     /translation="MPTGSIRPFGLTRLLVGTSLLVGCSSSSPGASPDAGGAGQVLRI
                     VPVSPTGHDHFFGVAFDPQG"
BASE COUNT          320 a          793 c          753 g          308 t
ORIGIN      
        1 cgctgcgcgc cacggccggc cggctcggcc tgatcgcgat cgacgaggct cactgtatct
       61 ccgagtgggg acacgacttt cggcccgact accgccggct cggcgatgcg gtgcggctgc
      121 tcggcccgcc gcggatcgcg gcgttcaccg ccacggcgac tcccgaggtc cggcgcgaca
      181 tcgcggccca gctggcgttc gcgtcgcccg cgctgcacgt ccgcggcttc gaccggccga
      241 acctgcacta cgcggtcgag cgggtcggcg gcggcgacga caagtcgagc gcgctggtcg
      301 agcggatccg cacgcgcgac ggcggtgtcg cgctggtcta cgccgcgacc cgcaagaacg
      361 ccgagaagta cgcgtccgcg ctcaagctcg ccgggatgcg ggcgcgggtc taccacgccg
      421 ggctcgacgg cgaggtccgc gagcgcgcgc aggacgtgtt catggccggc aagctcgacg
      481 cgatcgtcgc gaccaacgcg ttcggcatgg gggtcgacaa gtccgacatc cggctggtcg
      541 tgcacgccga catcccgcgc agccccgagg cgtactacca ggaggccggc cgcggcggcc
      601 gcgatggcaa gccgacccgc tgcgtgctgc tgttcaacca cagcgacatc aagctgcagg
      661 agttcctgat cgacgcgtcg tacccgtcgg ccgaggtgct gcgcggcgtg tggaagctgc
      721 tgcgcgaccg gccgcagctc ggccggctga cgccgtacga cgacgagctc gagaaccggc
      781 tgcgcaacca gctcggcggc gacgtcaacg gcgcgaccat gggcgcggcg ctgcggatcc
      841 tcgagcgcca cggcatgctg cggcgcgacg acgagaacct gatggcggcc cggcccgagc
      901 ccggcgtgtt cttgccgctc gacgtcgagt cgctgcagcg ccgcgccgac gtcgagcgga
      961 gcaagctgcg caccatggtc gagtacgcct actacccgcg ctgccgccgg cagttcgtgc
     1021 tcgagtactt cggcgaccag gactggagcg atcgcgaccg gcggtgcggc gcgtgcgaca
     1081 cctgcgaggc gatcgcgcac ggccggccga ccgcgatgca gctgtcgccc agcgagctcg
     1141 ccgcgatcaa gaacctgtta tcgctggtcg gcgcgctgca cggccggttc ggccggatgc
     1201 gcgtggtcaa gctcgcggtc ggcagcgacg acgacacgcg gttcgacgac ctgccggagc
     1261 gcggctgcct gcgcggctgg cgcgagcgcg ccgccctcga cctgctgcgc gcgctcgagg
     1321 gcgtcggcct ggtcgaggca tcgcgcggcg agtacccgac gatcgcgctg acccggcgcg
     1381 gcagccaggt cgccgccggc acgctcgacg ccggtacgct ggacgtccag atgccgagcc
     1441 ccggcaagca ggccaggtcg aagcgcagcc gcaagcgcta gcccgccccc gtcactcccc
     1501 gtcgggggct gccgccgtct ctggcgtctg ccggttcctg gccatctcct cggcgcgata
     1561 cgccgccgag atgcccccga tcaaggccag cgtgaacgcc agccgcagga ggatcgagaa
     1621 gatcctcgga aacgcgtcgc ccggactcat cacgaccacg gcgagctgga gccccagaac
     1681 acggccaggg cgaggatcag gctgtcgacg cgtggctggc cagcccgacc aggtccaggc
     1741 cgcggatcgg gtcaccgcgc tggcacagcc ggtgtgccct ggagcgcacg ccatgtagtt
     1801 tcccggctca gactctcgca gaacgggaat tgccagttcg ttctcgatgt cgccattcgg
     1861 gcgcgtagtg cgaggacgtg atccgcgcct tgccgcgatt cctgcttgcg aacatccagt
     1921 gtcactgaca acgattgatt ttcaaattca ataacaacct gacaatgcgc aggagctcca
     1981 catgcctaca ggttccatcc gccctttcgg tctcacccgg ctgctggtcg ggaccagcct
     2041 cctcgtcggg tgctcgtctt cctcccccgg cgcaagcccc gatgccggcg gcgccggcca
     2101 ggtgctgcgc atcgtgccgg tctccccgac cggccacgat cacttcttcg gtgtcgcgtt
     2161 cgacccgcag ggca
//
LOCUS       DASVOR010000625         1808 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_64350,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000625 DASVOR010000000
VERSION     DASVOR010000625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1808
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64350"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..929
                     /locus_tag="VF516_12330"
     CDS             63..929
                     /locus_tag="VF516_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX8108508.1"
                     /translation="MGIFSRLGTLIKSNINDLITKAEDPEKMLSQVLLEMQQQLVEAK
                     KAVAVAIADEKKLQKQYTAESDKAKEWERKAMVAVRAGDDNLARQALARKQEHESIAS
                     QFQQQWLSQKQAVEKLKDALRLLNNKIEEAKRKKNILIARKKRAEAQQQIANTMQGLG
                     DTSAFDTFDRMAERIQLMEAEAEAGAELAGELSGDTLESKFLQLEQGGAHSEDDALSE
                     LKAKMGMLEAPKGESAKQLGAGAAVGKDDKGSEGGSAPATAGLGGLTPEDLKELEDLD
                     LSELDAKEAKKA"
BASE COUNT          342 a          591 c          621 g          254 t
ORIGIN      
        1 agtagcacgc tcggagcgag cgttgcaacc acacctttga tcccgtacgc tcggtggaag
       61 ccatggggat cttttccaga ctcgggacgc tgatcaagtc caacatcaac gatctcatca
      121 cgaaggctga ggatcccgag aagatgctgt cccaggtgct gctcgagatg cagcagcagc
      181 tggtcgaggc caagaaggcg gtcgcggtcg cgatcgcgga cgagaaaaaa ctgcagaagc
      241 agtacaccgc cgagtccgac aaggccaagg agtgggagcg caaggcgatg gtcgcggtgc
      301 gcgccggtga cgacaacctc gcgcgtcagg cgctcgctcg taagcaggaa cacgaatcga
      361 tcgcctcgca gttccagcag cagtggctgt cgcagaagca ggcggtcgag aagctcaagg
      421 atgccctgcg cctgctgaac aacaagatcg aggaagccaa gcgcaagaag aacatcctga
      481 tcgctcgcaa gaagcgcgcg gaggcccagc agcagatcgc caacacgatg cagggcctcg
      541 gcgataccag cgcgttcgac accttcgacc gcatggccga gcggatccag ctgatggagg
      601 ccgaggccga ggcgggcgcc gagctcgccg gggagctgtc cggggacacc ctggagtcga
      661 agttcctcca gctcgagcag ggcggcgccc acagcgagga cgacgcgctg tccgagctca
      721 aggccaagat gggcatgctc gaggccccca agggcgagtc cgccaagcag ctcggcgccg
      781 gcgccgcggt cggcaaggac gacaagggtt cggagggcgg ctcggccccc gcgacggccg
      841 gcctcggcgg gctgaccccc gaggacctca aggagctcga ggacctcgac ctctcggagc
      901 tcgacgccaa ggaagccaag aaggcctagc cagcggccgg tttgatccgc gaggaagatc
      961 gaggagttgc cggaaggttc cgatcgcgcg cggcatcgca ggcgcgaatg tgtgctgcgc
     1021 gtggaggtct gatgattcat cacaagtcct gtcccatgtc gtcgtgatcg tcgacgtcga
     1081 cgtggacgtg gtcgttgact caggccgttg aggtggacgg cgacgtacga cgttgatccc
     1141 accgtcgacg ttggacgcga accagtcatc cgccgcaagc ctcgacggag agcggcaacc
     1201 gggatctaac gtcgacggtg gggtcagacg tgtacgtacc gcttcgctgc cgtcaacctc
     1261 aacggtgggg tcaaggtcga cgttcaggtc aaccgcatgt cccccaactc gtgacgatca
     1321 tcagacggta gagtcaaggt caaggtcgag gaccacggtg gccctctgcc ctcgccggga
     1381 tgtaggtgtc tgacagcggg cgcggcgcgc ggccgatcgc ctccgcgaag ggctagtgcc
     1441 cggtcgaggc gcgtccgttc tcgacgaaga tcggcgagat caggttgtgt cggaacggcg
     1501 cgcccccggt gtgaacgaat caccacagcc gcggctcggc cggccgtcgg gcctgcgatc
     1561 gccgccgcaa gccagcagaa gaccagtggt ttcctgcgcc agcgcggcgg ggggttccgc
     1621 cgatcgtcgc cggcggcaga cgctgccgcc ggatcgtggc ttgcagacgc ctggtccatg
     1681 cgcctcgtcg ccggtgtgct gtccctcgcc ctgttcgccc cgttcgcgcg cgccgacgac
     1741 accacgaccc cgccggcgcc gccgccggtc ccgacaccga gccccgcgcc gccggcaccg
     1801 cccgcgcc
//
LOCUS       DASVOR010000626        11984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_444095,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000626 DASVOR010000000
VERSION     DASVOR010000626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11984
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444095"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..368
                     /locus_tag="VF516_12335"
     CDS             171..368
                     /locus_tag="VF516_12335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108509.1"
                     /translation="MVSARGGKYGFVYPSRQRAYKALRRHGLTKSMAAAIANGGRTHA
                     QRSRMAHKAARTRKLRYGGRR"
     gene            497..652
                     /locus_tag="VF516_12340"
     CDS             497..652
                     /locus_tag="VF516_12340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108510.1"
                     /translation="MTINLAAIFYLVALVLAFIAAAFLPAHPRLLPAALGFVAAGLFC
                     SATGLGG"
     gene            complement(649..909)
                     /locus_tag="VF516_12345"
     CDS             complement(649..909)
                     /locus_tag="VF516_12345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108511.1"
                     /translation="MPDETPEEAAAIERIEVAISDYLKLHPGNRFLTSWVVLTSATSI
                     DSDGDEETRYAWIFPPRGLPWHTALGLMDIHRLLMTQRIADS"
     gene            complement(902..2404)
                     /locus_tag="VF516_12350"
     CDS             complement(902..2404)
                     /locus_tag="VF516_12350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108512.1"
                     /translation="MSEPEMGQIDERDTPEAARERAAWTRDIARMLREQTAVAPELRP
                     GYLAAAEWLDPSYDAPRGPGATEWLAQQFHEAYERLAPSYSYQTREASAKPWAEVPEN
                     NRQLMVATVDDLIRTGRLRPGSKFRPKYPDRNENYVPPRLDVERDSGARVEVSSPSGW
                     ITPEWMREGLATVADHIRPLWERAVAAEGTPIEVDLEPLFRWLKLYQDYAERDRPPRM
                     ARLELAGADELSGSNDSWWWVDVSGNGTHWSRFEGHHVQVDVELSTENDRDVNDWKGR
                     DEIRAGGKWLVKLNRQPVAGGYVGGDPLRTLEEIKRRIPELMNHAALHHSDPRPYAEQ
                     LMYRRVYYDRTPAVVVGTILDQGCVILRPVGVEAFPISLYDYDRPDESTDDPYERREA
                     KVELWYSPVHWWRDRLVEGEPAGPQEWAALIERERADEEAKYAAVRAEKFAREQPAQP
                     SGEAVPVVVEDDEDPEGTPTESEYQITEREADHEVWERQTGPLRGSLDDA"
     gene            complement(2401..3144)
                     /locus_tag="VF516_12355"
     CDS             complement(2401..3144)
                     /locus_tag="VF516_12355"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8108513.1"
                     /translation="MKAIIIARDRVTYTRACVLGLAATPGIDEIHIVDHGSTYEPMTA
                     YLGGIGGDRTFTNGGQARVHVHWRGNDHPRSLWTNGTLDVIVRHDERFLVTDCDIEPP
                     ADPGWLEHLGALLDLNPRAVKAGCGLRTDDLPDEYEHADRVRMWEAGYQGFGTLQRDS
                     ASGLAWFNASVDTTLALYRGLEPFNLDPAVRSADVRFMARHLSWYVDSANLAEDERYY
                     RAHALNGVSHWLDPDGYQSAAHLPEGVQP"
     gene            complement(3141..3797)
                     /locus_tag="VF516_12360"
     CDS             complement(3141..3797)
                     /locus_tag="VF516_12360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8108514.1"
                     /translation="MTLFDTTVVIPTHRSRGAVDDPASLLGQAVASVHAGLVVPRSII
                     ITEDTTGAGAAKTRQAALDLALGVGVNPADHIPTEWVSFLDSDDLWYPNHLATHRRLL
                     VGESGTEDLGDVAYSWFDGNGIAKDWEATHRGKTWDPRPGMGHHITMTITVRSAFARQ
                     ASFLGPELHPDWTGEDWRFILQLNELGARFVGTGELTWHYRVHGGNTSGSPQRGDALT
                     "
     gene            complement(3794..4090)
                     /locus_tag="VF516_12365"
     CDS             complement(3794..4090)
                     /locus_tag="VF516_12365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108515.1"
                     /translation="MTDTDPTPVVDVMPEFPLLAKDELAVDVIKYYRMCCESAGYLNQ
                     AAEVEKAIAEFNGWRERHPDKVKRPDHEHVPAGLRKPSRWQQLDVTAGEVTGRE"
     gene            complement(4201..4953)
                     /locus_tag="VF516_12370"
     CDS             complement(4201..4953)
                     /locus_tag="VF516_12370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108516.1"
                     /translation="MTDLDRVVLYSALYGRYEHPKPLPADLGCRAVMFVDDDEIFDEA
                     QAAGWEAWKVADPISPADIARAQAIDPAATAGMLRHKWWKTHPVEAGSMFGAKITLWV
                     DASLLITADRYAERCLEALGDDDWCAVPHPSRDCVYEEALFSATLARYHAGALAQQSA
                     AYVAMGHPRHWGLFATGANVRRHTPPVVEVSHEWWKECTGRSHQDQVSLPVLFRMAQD
                     IGLVDAGPLRWNARMPWARWWGVYEHGRMEKV"
     gene            complement(4983..5231)
                     /locus_tag="VF516_12375"
     CDS             complement(4983..5231)
                     /locus_tag="VF516_12375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108517.1"
                     /translation="MTDLISSDTPPNQREEQFFREEPTGLARELARMLNRASRENASN
                     TPDFILAAYMLDALEAGERLIRRRDRWYSMDPAPGGRR"
     gene            complement(5228..6076)
                     /locus_tag="VF516_12380"
     CDS             complement(5228..6076)
                     /locus_tag="VF516_12380"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8108518.1"
                     /translation="MRVVITGGAGFIGQATIAAVLDHGHTATTFDHPLDVCRPGDVQV
                     GIPVGTDAVIHLAGVLGTAELFDNPHLAVDVNVHGTLTVLERCAEIGAGYVGITMPPA
                     FPSIYTATKLCADRLASAFHLAHGVPVSRVRAFNAYGPGQKHGPSHPQKIIPTFATLA
                     WAGEPIPVWGDGEQTVDLIHAGDVGRMLADALRFGDDQVFDAGTGYPYTVNEIAEFVI
                     DYVSKRTGERSEVKHLPMRLGEQPTKIVAEGAGWDLLSWRPEFSREALVDTIEWYNPD
                     NPEDGR"
     gene            complement(6064..6786)
                     /locus_tag="VF516_12385"
     CDS             complement(6064..6786)
                     /locus_tag="VF516_12385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8108519.1"
                     /translation="MVTQPTPKHEFLHALHELLQPKTYLEIGVQSGTGMVQALPSTQS
                     IGVDPEPWGSAYLRVADHSPTAVIYPVTSDEFFANVVPSQCTPIELAFIDGMHLIENV
                     LRDFINVEQRAAPDGVIVIDDVLPYSAAITSREPLPGDWTGDVWKLWPILRKWRPELH
                     ITMVDVDPTGLMVVQALNLAGAIELATKLDKIIATWAPVDLGRDIADPEVFTARKHAV
                     SPAMALVEITKHRLEGTTSCAL"
     gene            complement(6786..6989)
                     /locus_tag="VF516_12390"
     CDS             complement(6786..6989)
                     /locus_tag="VF516_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108520.1"
                     /translation="MVIYAFAIDYGLNGHEIISVHDEKIDPEMEFRQWQANNAARGLM
                     QPYTTGYSLWRIDGDTGEIEELP"
     gene            complement(6983..7708)
                     /locus_tag="VF516_12395"
     CDS             complement(6983..7708)
                     /locus_tag="VF516_12395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8108521.1"
                     /translation="MRPFWTILRAAVIATHNRPAELRRCVEAIAPQVDLIVVVDNASD
                     PPAELSDAWSSLIEARIVLLRDPEQPPNLSRLWNLGISHAREYLRGGDCTPDATLGPF
                     DVAILNDDAVPPPGWFDAVAGAMREHGADAGSSDPFDFLPPGAVAVYRADTSPGVSTR
                     LAGWAMVLRGQWEGARFDERFRWWCGDDDMSHRARQAGGLVHVGGHLVLNTGADTSTG
                     GVLAEQSARDMQSFVDKWGMRPW"
     gene            complement(7791..8339)
                     /locus_tag="VF516_12400"
     CDS             complement(7791..8339)
                     /locus_tag="VF516_12400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108522.1"
                     /translation="MPQPKYRFNAGLFHSDMLTYEAVDPDAPLYDLTDPPDQHIVGPV
                     VASFRNELARYTYKPGVTLSVEPHEHDNMPLLRIVMEVPDSRNLTPVVPPLIEKRPNG
                     SVGPVMKRKIIRVVLNRRIPEDLTYREWGRDERAERFAWWLKQALEEVEVHEMREWLR
                     CDGELVDDPHAGPKPSPIGPIR"
     gene            complement(8357..9403)
                     /locus_tag="VF516_12405"
     CDS             complement(8357..9403)
                     /locus_tag="VF516_12405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8108523.1"
                     /translation="MTEPTTDQAGRPLKLFFWAAHLVDGCFRYRVEMPAAELARRGHE
                     VAVNMAMPDAIIDTADVVIGQRVHRSPPSVRWQLMAKRRDRYGSPRLVYEIDDDLLSI
                     DPRQNIFAGPFRDPVIRENIMDNMRCADMVTVTTEPLAEMARRYNANVAVIPNCFDES
                     IFDLSLPAYRGTRYVLTTLGWQGSPTHFEDWKVIQPVVAELAQADPALRVRFLGSVHP
                     AGIPESQIDFLKWTNDLTTHYKRVTRFDVGLSPLNGTIFNEAKSGLRYEEYSALGVPS
                     VCSDVPAYRPWVVHGETGFLARTPAEWRRYLLDLINDPGLRLKIGDAARAAARAWTIQ
                     ANGHRWEAAYRSLL"
     gene            complement(9415..9909)
                     /gene="ssb"
                     /locus_tag="VF516_12410"
     CDS             complement(9415..9909)
                     /gene="ssb"
                     /locus_tag="VF516_12410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8108524.1"
                     /translation="MSGAKITIVGNIGKDAPELRFTGTGVPVCVFSVADTPRILDKNK
                     QEWVDGETTWYRVNCWRDLAQNVAEGLAAGTRVIVTGSVRNRKYQDKEGNDRFSLEIE
                     ADAVGPDLKWRTVKVLDGVHEGAKKGGQRGPTEDPFESASTSRPAAQQQAQAQPAAAS
                     SGDW"
     gene            complement(9982..10323)
                     /locus_tag="VF516_12415"
     CDS             complement(9982..10323)
                     /locus_tag="VF516_12415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108525.1"
                     /translation="MGASKPKPVSDEPTIKLSAKQPKPGTPERGDGPLALRMMQGDDV
                     QYLLLVVAPREDARVRGSGARYVAAQMIAMEGMADDADRRDAEALYERAQRRRINGAN
                     PPPQQPMMDWE"
     gene            complement(10304..10633)
                     /locus_tag="VF516_12420"
     CDS             complement(10304..10633)
                     /locus_tag="VF516_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108526.1"
                     /translation="MKLTAYQIEGPYQEWENVAAWSLRGPVVTIRFEPGTVEPPIAEY
                     RLTRRVIGLADDGPPRPAYASRSVPTYERPHPRTDPRPARREPDDDFRWPAPPIRRER
                     HRGRQQT"
     gene            complement(10640..11374)
                     /locus_tag="VF516_12425"
     CDS             complement(10640..11374)
                     /locus_tag="VF516_12425"
                     /inference="COORDINATES: protein motif:HMM:NF005927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HEX8108527.1"
                     /translation="MTPWWREPAAIFDLETTGVNTRTDRVVSWCVGGVGHPLDSSLQP
                     RVQAELVNPGVPIPAGASAVHGIFDQHVRSTGNPPRESLARAVELLANAVKVGIPIMG
                     MNLAYDFSLLHFECLRHGLPTVEQVAGHPLAPVLDAYVLDQRVDRYRAGSRKLAAIAG
                     HYGVTLENAHDAVADAIASADVVYAIAERYPAIGNTGLYLLHLNQVEWKAEQAAGLQA
                     YKRRTDPSKVIDPCWPYCVNPQHTSG"
     gene            complement(11371..11673)
                     /locus_tag="VF516_12430"
     CDS             complement(11371..11673)
                     /locus_tag="VF516_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108528.1"
                     /translation="MGLTTRLCPSPRCRREVVTLLVTGQTGAHKEEVVDPDPTTWDGG
                     GRIRLMTHQPDTAKYPLGRRLQHAGQAFGATGLYRPHSTTCGGGTGRAAPRNREHG"
     gene            complement(11664..11831)
                     /locus_tag="VF516_12435"
     CDS             complement(11664..11831)
                     /locus_tag="VF516_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108529.1"
                     /translation="MNPTTSPSGPPLYGTYHNLPAWLVALILFGAVVFFLLLAWGASR
                     ATGRGSGTTWD"
BASE COUNT         1693 a         4143 c         4202 g         1946 t
ORIGIN      
        1 cgtgctggtg ctggccgtgg ccctcgtcgg gcacgcggcc ggcatctggt agaccgccgg
       61 ccacccaccg caaccgatcc tcccggttcc atggagctgc ccaatcccgg gcgcacggcc
      121 ggcgcggtgg gtggccggtc cgcgtacgct gggcgctgga ggtggtcgcc atggtgagcg
      181 cacgcggcgg gaagtacggg ttcgtctacc cctccaggca gcgcgcctac aaggcgttgc
      241 gccggcacgg actcaccaag agcatggccg ccgcgatcgc caacggcggg cgcacgcacg
      301 cccagcgctc gcggatggcc cacaaggcgg cccggacccg caagctgcgc tacggcggcc
      361 ggcggtaggt ctacgggcgt cccgtggcgc ggacgggacg cctggggtag tgccgggggg
      421 ccagccgcgc cgcagcgctc tccggccaac gtgacgggcc gagtaacgat cacggccccc
      481 gggtaacccg agggccatga ccatcaatct cgccgcgatc ttctacctgg tggcgctggt
      541 gctcgcgttc atcgccgccg cgttcctgcc ggcacacccg cgcctactgc cggccgcgct
      601 cggcttcgtc gccgccggcc tgttctgttc ggccaccggc ctcggcggct agctgtcggc
      661 gatccgctgt gtcatcagca gtcggtggat atccatcaac cccagcgcgg tgtgccacgg
      721 caggccacga ggcgggaaga tccacgcata ccgggtttcc tcgtcgccgt cgctgtcgat
      781 gctcgtggcc gaggtgagca ccacccacga ggtcagaaag cgattccccg ggtggagctt
      841 gagatagtcg ctgatcgcca cctcgatgcg ctcgatggcg gcggcctcct ccggggtctc
      901 gtcaggcatc gtcgaggctg ccgcgcagcg ggccggtctg gcgctcccac acctcgtgat
      961 cggcctcgcg ctcggtgatc tggtactccg actcggtggg cgtgccctcc gggtcctcgt
     1021 cgtcctcgac gacgaccgga acggcctcgc ccgacggctg ggccggctgc tcgcgggcga
     1081 acttctcggc gcggaccgcc gcgtacttcg cttcctcgtc cgcgcgctcc cgctcgatca
     1141 gcgccgccca ctcctgcggg ccggccggct cgccctcgac gaggcggtcc cgccaccagt
     1201 gcaccggcga ataccacagc tcgaccttcg cttcacgccg ctcgtacggg tcgtcggtcg
     1261 actcgtccgg gcggtcgtag tcgtacagcg agatcgggaa tgcctcgacg ccgaccggac
     1321 gcaggatcac gcacccctga tcgaggatcg tgccgaccac cacggcgggc gtgcggtcgt
     1381 agtagacgcg ccggtacatg agctgctccg cgtaggggcg cgggtcgctg tggtgcagcg
     1441 ccgcgtgatt catcagctcg gggatccgcc gcttgatctc ctccagggtg cgcagcgggt
     1501 cgccaccgac gtacccgccg gccaccggct ggcggttcag cttgaccagc cacttgccgc
     1561 ccgcgcggat ctcgtcgcgg cccttccagt cgttcacgtc gcggtcgttc tcggtggaca
     1621 gctcgacgtc gacctgcacg tggtgaccct cgaaccggga ccagtgggtg ccgttgccgg
     1681 acacgtcgac ccaccaccac gagtcattgc tgccgctcag ttcgtcggcg ccggccagct
     1741 ccaggcgggc catgcgcggc ggccggtcgc gttcggcata gtcctggtag agcttcagcc
     1801 agcggaacag gggttccagg tcgacctcga tcggcgtccc ctcggcggcc accgcccgct
     1861 cccacagggg ccggatgtgg tcggccacgg tggcgaggcc ttcacgcatc cactccgggg
     1921 tgatccatcc gctgggcgaa ctcacctcga cccgcgcgcc gctgtcgcgc tcgacgtcga
     1981 ggcggggcgg aacgtagttt tcgttgcggt ccgggtactt cggccggaac ttcgacccgg
     2041 gccgcaggcg gccggtccgg atcaggtcgt ccaccgtcgc caccatgagc tgacgattgt
     2101 tctcgggcac ctccgcccac ggcttcgccg acgcctcccg cgtctggtag ctgtagctcg
     2161 gcgccagccg ctcatacgcc tcgtggaact gctgggccag ccactcggtc gcccctggcc
     2221 ccctgggcgc atcgtaggag gggtcaagcc actcggccgc cgcgaggtag ccggggcgca
     2281 gctccggcgc cacggcggtt tgctcgcgca gcatgcgggc gatgtcacgg gtccaggccg
     2341 cgcgctcgcg tgccgcctcc ggggtgtcgc gctcgtcgat ctggcccatc tccggctcgc
     2401 tcacggctgg accccttcgg gcaggtgggc ggcgctctgg tagccgtccg ggtccagcca
     2461 gtgcgatacc ccgttcaggg cgtgcgcccg gtagtaccgc tcgtcctcgg ccaggttggc
     2521 cgagtcgaca taccacgaca ggtggcgggc catgaagcgg acgtcggcgg aacgcaccgc
     2581 cgggtccagg ttgaacggct ccaggccccg atacagggca agggtggtgt cgacgctggc
     2641 gttgaaccag gccagcccgg aagcgctgtc gcgctggagg gtgccgaagc cctgatagcc
     2701 ggcctcccac atgcgcaccc ggtccgcgtg ctcgtactcg tcgggcaggt cgtcggtacg
     2761 cagcccacag ccggccttca ccgcgcgcgg gttgaggtcg agcagcgcgc cgaggtgctc
     2821 cagccaaccc gggtcggccg gtggctcgat gtcgcagtcg gtgaccagga accgctcatc
     2881 gtggcggacg atcacgtcga gggtgccgtt ggtccacagc gagcgcgggt ggtcgttgcc
     2941 gcgccagtgc acatgcaccc gggcctggcc gccgttggtg aaggtccggt cgccgccgat
     3001 gccgccgagg taggccgtca tcggctcgta cgtcgagccg tggtcgacga tgtgaatctc
     3061 gtcgatgccg ggggtggccg cgaggccgag cacgcaggcg cgcgtgtagg tcacccggtc
     3121 gcgggcaatg atgatcgcct tcatgtcagt gcgtctcctc gttgggggga tccggacgtg
     3181 ttgccgccgt gcacacggta atgccaggtc agctcgccgg tgccgacgaa gcgggcgccc
     3241 agctcgttga gctggagaat gaaccgccag tcttcgccgg tccagtccgg atgcagctcc
     3301 ggacccagga agctggcctg gcgcgcgaac gccgagcgta ccgtgatggt catcgtgatg
     3361 tggtggccca tgccggggcg cgggtcccag gtcttgcccc gatgggtggc ctcccagtcc
     3421 ttggcgatgc cgtttccgtc gaaccaggag tacgcgacgt cgccgaggtc ttccgtgccg
     3481 gactcgccga cgagcagccg gcggtgagtg gccagatggt tggggtacca caggtcgtca
     3541 ctgtccagaa aggacaccca ctcggtcggg atgtggtcgg ccgggttgac gccgacacca
     3601 agggccagat cgagcgcggc ctgccgggtc ttcgccgcgc cggccccggt cgtgtcctcc
     3661 gtgatgatga tcgagcgggg cacgaccagg ccggcgtgca cgctggcgac ggcctggccg
     3721 agcagcgagg ccgggtcgtc gaccgcgccc cggctgcggt gggtggggat gaccaccgtg
     3781 gtgtcgaaca gggtcattcc cgcccggtga cctctccggc ggtcacgtcg agctgctgcc
     3841 agcgggacgg cttgcgcagg cccgccggga cgtgctcgtg gtcggggcgc ttgaccttgt
     3901 ccgggtggcg ctcccgccag ccgttgaact cggcgatggc cttctcgacc tcggcggcct
     3961 ggttgaggta gccggcgctc tcgcagcaca tcctgtagta cttgatcacg tcgaccgcga
     4021 gttcgtcctt ggccagcaag gggaactcgg gcatgacgtc gaccacgggg gtcgggtcgg
     4081 tgtcggtcat ggaagatcat cccttcagac tgctgtaaca catggcgagc gcggccagcg
     4141 cgaacgccgc cactaccaca tagatgaact tgtcgaacag gctggggccg cgccgcgcca
     4201 tcagaccttc tccatcctgc cgtgttcgta gacgccccac catcgtgccc acggcatgcg
     4261 ggcgttccag cgcagcggtc cggcgtcgac caagccgata tcctgcgcca tccgaaacag
     4321 caccggcaag ctcacctgat cctgatggct gcgcccggtg cattccttcc accactcgtg
     4381 cgacacctcg accacgggcg gggtgtgtcg gcggacgttg gcgccggtgg cgaacaggcc
     4441 ccagtgccga gggtgcccca tcgccacgta ggcggcggac tgctgagcca gcgcccccgc
     4501 gtggtaccgc gcgagggtgg ccgagaacag ggcctcttcg tagacgcagt cccgcgacgg
     4561 atgcggcacc gcacaccagt cgtcatcgcc gagcgcctca aggcaacgct cggcgtaccg
     4621 gtcggcggtg atgagcagtg aggcgtcgac ccacagcgtg atcttcgcgc cgaacatcga
     4681 gcccgcctcg accgggtggg tcttccacca tttgtgtcgc agcatgccgg ccgtcgccgc
     4741 cgggtcgatg gcctgcgccc gcgcgatgtc ggccgggctg atcgggtcgg cgaccttcca
     4801 cgcttcccag ccggcggcct gcgcctcgtc gaagatctcg tcatcatcga cgaacatcac
     4861 ggcccgacag ccgaggtcgg cgggcagtgg cttggggtgc tcgtaccggc cgtacagggc
     4921 gctgtacagc accacgcggt cgaggtcggt caccggccgt ccggggcgcc atccctccgc
     4981 gctcaccgtc gaccccccgg cgccgggtcc atgctgtacc agcggtcgcg gcgccggatc
     5041 agccgctcgc ccgcttcgag cgcgtcgagc atgtaggcgg ccaagatgaa gtcaggcgtg
     5101 ttactggcgt tctcgcggct ggcccggttg agcatgcgtg ccaactcccg cgcgaggccg
     5161 gtaggctcct cgcggaaaaa ctgctcctcg cgctggttcg gcggggtgtc ggagctgatg
     5221 agatcggtca ccggccgtcc tccgggttgt cggggttgta ccactcgatc gtgtcgacga
     5281 gcgcttcccg ggagaactcc ggccgccacg acagcaggtc ccagccggcg ccttcggcga
     5341 caatcttggt gggctgctcg ccgaggcgca tcggcaggtg tttgacctcg gagcgctcgc
     5401 cggtccgctt cgacacgtag tcgatcacga attccgcgat ctcgttcacc gtgtacgggt
     5461 agccggtgcc cgcgtcgaag acctgatcgt cgccgaagcg cagcgcgtcg gcgagcatcc
     5521 gtccgacgtc gcccgcgtgg atcaggtcga cggtctgctc accgtcgccc cacaccggaa
     5581 tcggctcgcc cgcccacgcc agcgtggcga acgtcgggat gatcttctgg gggtggctgg
     5641 ggccgtgctt ctggccgggg ccgtaggcgt tgaacgcccg cacccgcgac accggcacgc
     5701 cgtgcgcgag gtggaacgcg ctagcgagac ggtcggcaca gagcttcgta gcggtgtaga
     5761 tcgagggaaa ggccggcggc atcgtgatgc cgacgtagcc ggcgccgatc tcggcgcagc
     5821 gctccagcac ggtgagggtg ccgtgcacgt tgacgtcgac cgccaggtgc gggttgtcga
     5881 acagctccgc cgtgccgagc acacccgcca gatggatgac cgcgtcagta ccgaccggga
     5941 tgccgacctg gacatccccg ggcctgcaca cgtcgagcgg gtgatcgaag gtggttgcgg
     6001 tgtggccgtg gtcgagcacg gccgcgatgg tggcctgccc gatgaagccg gcaccgccgg
     6061 tgatcacaac gcgcatgagg tggtcccctc cagtcggtgt ttggtgatct ccaccagggc
     6121 catggccggg gagacggcat gcttacgggc ggtgaacacc tccgggtcgg cgatgtcccg
     6181 gccgaggtcg accggcgccc aggtggcgat gatcttatcg agcttcgtgg ccagctcgat
     6241 cgcgccggcc agattcagag cctgcacgac catgaggccg gtcgggtcga cgtccaccat
     6301 ggtgatatgc agctcgggcc gccacttgcg caggatcggc cacagcttcc acacgtcgcc
     6361 ggtccagtcg cccggcagtg gctcccgcga ggtgatcgcc gccgagtacg gcagtacgtc
     6421 atcgatcacg atcaccccgt cgggagccgc gcgctgctcg acgttgatga agtcccgtag
     6481 cacgttctcg atgaggtgca taccgtcgat gaacgccagc tcgatcggcg tgcactgcga
     6541 cggcaccacg ttggcgaaga actcgtcgga ggtgaccgga tagatcaccg ccgtgggcga
     6601 gtggtcggcc acccgcaggt atgccgaacc ccacggttcg ggatcgacgc cgatagactg
     6661 ggtggacggc agcgcctgta ccatgccggt cccggactgc accccgattt cgagatacgt
     6721 cttgggttgc aacagctcgt gcaacgcgtg caagaactcg tgcttcgggg tcggttgggt
     6781 caccattatg gaagctcctc gatttcgccc gtgtcgccgt cgatccgcca cagtgaatag
     6841 cccgtggtgt acggctgcat gagcccgcgt gcggcgttat ttgcctgcca ctggcgaaac
     6901 tccatctcgg gatcgatctt ttcgtcgtgc acgctgatga tctcgtggcc gttgagtccg
     6961 tagtcgatgg cgaacgcgta gatcaccacg gacgcatccc ccatttgtcc acaaaggatt
     7021 gcatgtcgcg ggcggactgc tcggcgagca ccccgccagt cgaggtgtcg gcgcccgtgt
     7081 tcagcaccag gtggccgccc acgtggacga ggccgccggc ctggcgggcg cggtggctca
     7141 tgtcgtcgtc gccgcaccac cagcggaacc gctcgtcgaa gcgggcaccc tcccactggc
     7201 cacgcagcac catcgcccac ccggcgagac gcgtcgacac gcctggcgac gtgtcggccc
     7261 ggtagacggc caccgcgccg ggcggcagga agtcgaacgg gtcggagctg cccgcgtcgg
     7321 cgccgtgctc gcgcatcgcg ccggccaccg cgtcgaacca gccgggcggc ggtaccgcgt
     7381 cgtcattgag gatggccaca tcgaaggggc cgagtgtggc gtccggcgtg cagtcaccac
     7441 cccggaggta ctccctcgcg tggcttatgc cgaggttcca gagccgggac aggttcggcg
     7501 gctgctcggg gtcgcgcagc agaacaatgc gggcttcgat cagtgacgac caggcgtccg
     7561 acagttcggc gggcggatcg ctggcgttgt cgacgaccac gatcaggtcg acctgcgggg
     7621 cgatcgcctc gacgcaccgg cgcagctccg ccggccggtt gtgggtggcg atgacagccg
     7681 cccgcaggat ggtccagaac ggcctcaacg ggaccgcctc ccgagccacc acaccggcag
     7741 cccgagcagc gccagcaagc ccaggaacgc gaccaggacg gtcgggccgc tcaccggatc
     7801 ggcccgatcg gcgagggctt cggcccggcg tgcgggtcgt cgacaagctc gccgtcgcag
     7861 cgcagccact cccgcatctc gtgaacctcg acctcttcca gagcctgttt cagccaccag
     7921 gcgaaccgct ccgcccgctc gtcgcggccc cactcccggt aggtcaggtc ttccgggatg
     7981 cgacggttaa gcaccacccg gatgatcttg cgcttcatga ccggaccgac cgacccgttc
     8041 ggccgcttct cgatcagcgg cggcacgacc ggggtcaggt tgcgcgagtc ggggacctcc
     8101 atcacgatcc gcaacagggg catgttgtcg tgctcgtgcg gctcgacact caaggtgacg
     8161 cccggcttgt aggtatagcg ggccagctcg ttgcggaacg acgcgaccac cggcccgacg
     8221 atgtgctggt cgggcgggtc ggtcaggtcg tagagcgggg cgtccgggtc gacggcctcg
     8281 taggtgagca tgtcgctgtg gaacaggccg gcgttgaaac ggtacttggg ctgaggcatg
     8341 ggggatgagt ccttcttcac agcagggaac ggtacgcggc ttcccatcgg tggccgttgg
     8401 cctggattgt ccacgcgcgg gccgccgcgc gggccgcgtc gccgatcttg aggcgcaggc
     8461 ccgggtcgtt gatgaggtcg agcaggtacc ggcgccactc ggccggggtg cgggccagaa
     8521 agccggtctc gccgtgcacc acccacggac ggtacgcggg cacgtcggag cacaccgacg
     8581 gcacgccgag ggccgaatac tcctcgtagc gcagcccgga cttcgcctcg ttgaagatcg
     8641 tcccgttcag tggcgacaac cccacatcga aacgggtgac ccgcttgtag tgggtggtca
     8701 ggtcattcgt ccacttcagg aagtcgatct gtgactcggg gatgccggcc ggatggaccg
     8761 agcccaggaa gcgcacccgc agcgcgggat cggcctgggc cagctcggcg accaccggct
     8821 gaatgacctt ccagtcctcg aaatgcgttg gcgagccctg ccacccgagg gtggtcagca
     8881 cgtaccgcgt gccccggtag gcgggcagcg acagatcgaa gatcgactca tcgaaacagt
     8941 tcgggatgac cgccacgttg gcgttgtagc ggcgagccat ctcggccagc ggctccgtgg
     9001 tcacggtgac catgtccgcg caccgcatgt tgtccatgat gttctcgcgg atgaccgggt
     9061 cacggaacgg cccggcgaag atgttctgac gcgggtcgat ggacagcaga tcgtcgtcga
     9121 tctcgtagac gagccggggg ctgccgtacc ggtcgcggcg cttcgccatg agctgccacc
     9181 ggaccgacgg cgggctgcgg tggacgcgct ggccgatcac gacgtcagcg gtgtcgatga
     9241 tcgcatccgg catcgccatg ttcaccgcga cctcgtggcc gcgtcgggcc agctcggccg
     9301 ccggcatctc gacccggtac cggaagcagc cgtcgaccag gtgggcggcc cagaagaaca
     9361 gcttcagggg tcgcccagcc tggtcggtgg ttggttcggt catggcgggg cggcttacca
     9421 gtcgccggac gaggccgccg ccggctgtgc ctgggcctgc tgctgcgcgg ccggccggct
     9481 ggtcgacgcg gactcgaacg ggtcctcggt cgggccgcgc tggccgccct tcttcgcacc
     9541 ctcgtggacg ccgtcgagca ccttgacggt ccgccacttc aggtcaggcc ccaccgcgtc
     9601 ggcctcgatc tccagggaga accggtcgtt gccctccttg tcctggtact tgcggttacg
     9661 caccgagccg gtgacgatga cccgcgtacc ggcggcgagg ccctccgcca cgttctgggc
     9721 caggtcgcgc cagcagttga cccggtacca cgtggtctcg ccgtcgaccc actcctgctt
     9781 gttcttgtcc aggatccggg gcgtgtcggc caccgagaac acgcacacgg gcacaccggt
     9841 gccggtgaag cgcagctcgg gggcgtcctt gccgatgttg ccaacaatcg tgatcttcgc
     9901 tccgctcatc gcggggtgat ctcctctcgg gaagggtggt ctgatctcac cagtccgggc
     9961 ctgggcttgt caaccgacgt gtcactccca gtccatcatg ggctgttggg gcggcgggtt
    10021 ggcgccgttg atccggcgcc gctgcgcccg ctcgtacagc gcttcggcgt cgcggcggtc
    10081 cgcgtcgtcg gccatgccct ccatggcgat catctgggct gccacgtacc gcgcgccgga
    10141 cccgcgcacc cgggcgtcct cccggggtgc gacgaccagt agcagatact gcacgtcgtc
    10201 cccctgcatc atgcgcagcg cgagcggccc gtctccgcgc tccggggtgc ccggcttcgg
    10261 ttgcttcgcc gacagcttga tcgtcggctc gtcgctgacc ggcttaggtt tgctggcgcc
    10321 cacggtggcg ctccctcctg atcggtggtg ccggccagcg gaagtcgtcg tcgggctccc
    10381 ggcgggccgg tcgggggtcg gtgcgggggt gtggacgctc gtacgtcggc accgaacgtg
    10441 acgcgtacgc gggccggggc gggccgtcgt cggccaaccc gatgacgcgc cgggtcaacc
    10501 ggtattcggc gatcggcggc tcgacggtgc caggctcgaa acggatggtg acgaccgggc
    10561 cgcgcaggct ccaggccgcc acgttctccc actcctggta cggtccctcg atctggtagg
    10621 cggtgagttt catcgattcc tatccggagg tgtgctgggg gttgacacag tacggccagc
    10681 aggggtcgat gaccttggac gggtcggtgc ggcgcttgta cgcctgaagt ccggccgcct
    10741 gctcggcctt ccactccacc tggttgaggt gcagcaggta caggcccgtg ttgccgatcg
    10801 cggggtaccg ctcggcgatg gcgtacacca catccgccga cgcgatcgcg tcggcgaccg
    10861 cgtcatgggc gttctccagc gtcacgccgt aatgcccggc tatcgcggcg agcttgcggg
    10921 agccggcccg gtaccggtcg acccgctgat cgagcacgta ggcgtcgagc accggtgcca
    10981 gcgggtggcc ggccacctgc tcgacggtcg gcaggccgtg gcgcaggcac tcgaagtgca
    11041 gcagcgagaa gtcgtacgcc agattcatgc ccatgatcgg tattccgacc ttgaccgcgt
    11101 tggcgagcag ctccaccgcc cgcgcgagac tctccctggg cgggttgccg gtcgagcgca
    11161 cgtgctggtc gaagatgccg tgcaccgcgc tggcgccggc cgggatcggc acccccgggt
    11221 tgaccagttc ggcttgcacc cggggttgca ggctggagtc gagcggatgc ccgaccccgc
    11281 ccacacacca cgacaccacc cggtccgtac gggtgttcac cccggtggtt tccaggtcga
    11341 agatggcggc cggctcccgc caccacggcg tcaaccgtgc tcccggttcc gtggcgccgc
    11401 acggcccgtg ccgcccccgc acgtggtgct gtgcggccgg tacaggccgg tcgcgccgaa
    11461 cgcctggccg gcatgctgga gccgccggcc gagggggtac ttggccgtgt ccggttggtg
    11521 ggtcatgagc cggatacggc cgccgccgtc ccaggtggtc ggatccgggt cgacgacctc
    11581 ttccttgtgg gcgccggtct gcccggtgac cagcagggtg accacctcgc gccggcagcg
    11641 tggcgacgga cacaggcggg tggtcagtcc catgtcgtgc cgctcccccg cccggtggcc
    11701 cgggaggcac cccaggccag caacaggaag aacaccacgg ccccgaacag gatgagggca
    11761 accagccagg cgggcaggtt gtggtacgtg ccgtacagcg gcgggcctga cggcgaggtg
    11821 gtcgggttca ccggatctgg cccgaccgca acgcctccag gaacgcgtgg atctgcgcgc
    11881 cggtggcctc gctggtcttc tgcaccccgc agaaggtccg cagccggtcg tggacgtcgg
    11941 cggtcgtcca gccggcgggc gcggcggcga acaccatggc gacc
//
LOCUS       DASVOR010000627         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_414500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000627 DASVOR010000000
VERSION     DASVOR010000627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VF516_12440"
     CDS             complement(<1..213)
                     /locus_tag="VF516_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108530.1"
                     /translation="MSSPVTLPAAHATVPSRGFRSPGDAVRADVGRAIAMAAGGAAAF
                     AAVEYPLTLASYAGAPRWVARLELIAL"
     gene            567..>1978
                     /locus_tag="VF516_12445"
     CDS             567..>1978
                     /locus_tag="VF516_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108531.1"
                     /translation="MTWIAKGFVRHGWLAAIAVAYLYVFPFYPQIHSANELPRVYLVQ
                     AIVEDHTFAIDRGVAMWGATADVSPSGGHLYSNKAPGSSLLAVPAYAAVRAIAGPPGL
                     VATVWICRVFAGVLPMLGFLWLLWGFLARWAPDPAARRVVVLGYALGSLAMTYSILFY
                     SHQLGAICIASAWILAVDVVERRRPIAAMAAAGLLAGAAPLVDYQAVFAALPVAVYVV
                     ARLRPSAQLVRAIALAAAGAAPPIALLLAYHAICFGSPWRTGYDASVAFAVFHQHGFL
                     GITELRWEAFWGSLVRPDNGLVALSPGWLLAIPGGVVLWRRGERATCAIAAGAAVIYV
                     LFISSINFWRGGWEVGPRYITAMLPFLLPPIAALVQDWRGRPVRFGLAAGLLGVGVAV
                     YVLSSATFPYWPDSVRHPLYDVAFRLLGEHLTAPNLGRALGAGGILGLVPYLAVAFGA
                     FGLAIARAAGARSLAIAAVVT"
BASE COUNT          220 a          696 c          731 g          331 t
ORIGIN      
        1 cagcgcgatg agctcgagcc gggcgaccca gcgcggggcg ccggcgtagc tcgccagggt
       61 cagcgggtac tcgaccgccg cgaacgccgc cgcgccgccg gcggccatgg cgatcgcgcg
      121 gccgacgtcg gcgcgcaccg cgtcgcccgg gcttcgaaag ccacgggatg gcaccgtcgc
      181 gtgggcggcc ggcaaggtca cgggcgacga cacgatcgat ccggtggtat cacgttcccg
      241 agcacgtgca catgcatccc ccgaagctcg cctgcggagg caacctcgac gtcgaccttg
      301 aggtcgacct tgactccagc gttgagcttg acggcagcga agcgatacgt ggacgtctga
      361 ctccaccgtg gacctcgacc ttgccccgtc gtggcggtcc ctcgaggctc gcagcacagg
      421 atcggtcgac gtcgagcgtc gacggtggac tcgacgtcgt acgtcgccgt caacctcgac
      481 gctccgggtc aacgatcagg tcgacgtcca cgatcacgaa cgccgtgtct accccaaccc
      541 cgaccctttg atcagaacct ctcgagatga cctggatcgc aaagggtttc gtgcgccacg
      601 gctggctcgc cgcgatcgcg gtcgcgtacc tctacgtgtt cccgttctac ccgcagatcc
      661 acagcgcgaa cgagctgccg cgggtctacc tggtccaggc gatcgtcgag gatcacacgt
      721 tcgcgatcga ccgcggcgtg gcgatgtggg gcgcgacggc cgatgtctcg ccgtcgggcg
      781 gccacctgta ctcgaacaag gcgccgggca gctcgctgct cgcggtgccg gcgtacgcgg
      841 cggtgcgcgc gatcgccggc ccacccgggc tcgtcgcgac ggtgtggatc tgccgggtgt
      901 tcgccggcgt gctgccgatg ctcgggttcc tgtggctctt gtggggcttc ctggcccggt
      961 gggcccccga tcccgcggcg cggcgcgtgg tcgtgctggg ctatgcgctg gggtccctgg
     1021 cgatgaccta ctcgatcctg ttctactcgc accagctcgg cgcgatctgc atcgcgagcg
     1081 cgtggatcct cgcggtcgac gtcgtcgagc ggcggcggcc gatcgcggcg atggccgcgg
     1141 ccgggctgct ggcaggggcg gcgccgctgg tcgactacca ggcggtgttc gcggcgttgc
     1201 cggtcgcggt gtacgtcgtg gccaggctcc ggccgagcgc gcagctggtg cgcgcgatcg
     1261 cgctcgccgc ggccggcgcc gcgccgccga tcgcgctgct gctcgcgtat cacgcgatct
     1321 gcttcggcag cccgtggcgc accgggtatg atgcgtcggt cgcgttcgcg gtgttccacc
     1381 agcacggctt cctcgggatc accgagctgc gctgggaggc gttctggggc tcgctggtcc
     1441 gcccggacaa cgggctggtc gcgctgtcgc cgggctggct gctcgcgatc ccgggcgggg
     1501 tcgtgctgtg gcggcgcggc gagcgggcga cctgcgcgat cgcggccggc gcggcggtga
     1561 tctacgtgct gttcatctcg tcgatcaact tctggcgcgg cggctgggag gtcgggccgc
     1621 gctacatcac cgcgatgctg ccgttcctgc tgccgccgat cgcggcgctg gtccaggact
     1681 ggcggggccg gccggtccgg ttcgggctcg cggcggggct gctcggcgtc ggggtcgcgg
     1741 tgtacgtgct gtcgtctgcg acgttcccgt actggcccga cagcgtccgc cacccgctgt
     1801 acgacgtcgc gtttcgcttg ctcggggaac atctcaccgc gcccaacctc ggccgcgcgc
     1861 tcggcgccgg cggcatcctg gggctcgtcc cgtacctcgc ggtggcgttc ggggcgttcg
     1921 gcctggcgat cgcgcgcgcc gcgggagcgc gcagcctcgc gatcgcggcg gtggtgac
//
LOCUS       DASVOR010000628         4624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_502783,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000628 DASVOR010000000
VERSION     DASVOR010000628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502783"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1508
                     /gene="rny"
                     /locus_tag="VF516_12450"
     CDS             <1..1508
                     /gene="rny"
                     /locus_tag="VF516_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEX8108532.1"
                     /translation="GRARGDAGGGQRAAAEEAEKIRAASRVEREAVKQAAEVEGKEAA
                     RKRRAELDEELRARRTELARREDGLAQREREVEKTRREAERRAAELDKKEKSIDGKIK
                     QADSAIEKAEAAQGEARKKLEEIAGLSEEQARKKLEDEVRGQALTSAAAEIKRIEDDA
                     HKEATARAKTIVATAIQRFASEFVHERTVAVVPLPSDDLKGRLIGREGRNIRALEAAT
                     GIDMIIDDTAESITISCFNPVRREIARLAISALVADGRIHPTRIEEVVRKAEKEVEKL
                     CKEAGEQAVFDLGLHRVHPELVKLLGRLKFRQSYAQNVLMHSVEVGYLSGLIAAELGA
                     NVKLARRAGLLHDIGKAIDHEHEGAHAEVGAQLARKHQESPRVCQAIASHHGDEGGAQ
                     PQALLDHIVAAANSLSAARPGARREQLASYIKRLDDLEAICKRFGGVERAYALQAGRE
                     IRVMVMNDQVDDAQAIVMSKEIARAIENEATYPGQVRVVVVRETRASDYAR"
     gene            complement(1639..1920)
                     /locus_tag="VF516_12455"
     CDS             complement(1639..1920)
                     /locus_tag="VF516_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108533.1"
                     /translation="MLRSTSAVRAGSPRRWAAGAVIAATLLAEVPHAPDRTPDHAPDG
                     AADPCAACDRSAAGAPPRSPPAPVVPLPAAASGSVGTSHRPTLRHVIGL"
     gene            complement(1933..2343)
                     /locus_tag="VF516_12460"
     CDS             complement(1933..2343)
                     /locus_tag="VF516_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108534.1"
                     /translation="MTHTQATSGRSSTSSSHPSPGTRPRTVTRPVALPATRAEALELR
                     DLLEANLQALRALHEEHRRMLELNLRTVDSASDLRRELRAIGSPRAPWVRALSETRAL
                     LFVALRELIIGANQAELSMHLPKQPPADPRRPER"
     gene            2414..3526
                     /locus_tag="VF516_12465"
     CDS             2414..3526
                     /locus_tag="VF516_12465"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8108535.1"
                     /translation="MYRRGDLSHVARTLRVLDELRGFRRGRRLSEIAAALGASERTVR
                     RDLADLRSAGFEIESLAIDGRAAARLVERTYSSIAITRRERYTLLAIASLFDVLRGTP
                     LWEDVSSLQRKLEQRMTPEERAEHASLGDRFAYVPDGGTKAYEGKEDVLDALLTGVLS
                     RRVVRFAYRDARGRARGGHLAPYTMLLYKHGLYVVGCRLREPGDGRAIAPGTPVAVWA
                     VERFTDAEALRKAPFTAPPGFRIGELLNGAFGIHVTGDAAPHRVVIEFSAEKAAFVRA
                     RVWHPTQTFEELADHRVRLAFTCVSLMPVVSWVLEWGPPARAIEPPELVAEVIRELEA
                     ARRAYPGDVPHGRGDAGDRAAESRQVGARHAAAGES"
     gene            complement(3487..3864)
                     /locus_tag="VF516_12470"
     CDS             complement(3487..3864)
                     /locus_tag="VF516_12470"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8108536.1"
                     /translation="MPTRERVQELIDAVVNGDHAEAIARFYTDDASMQENAAEPRRGL
                     AHLVERERKVTAQVARIVSHRPEFVAIDGDRVAIHWVFEFTHPDGRIRVIDEIAMQIW
                     DGDKVARERFFYDSPAAAWRAPT"
     gene            3946..>4624
                     /locus_tag="VF516_12475"
     CDS             3946..>4624
                     /locus_tag="VF516_12475"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8108537.1"
                     /translation="MEYTKLGATGLEVSRICLGCMSFGAPDRGNHTWTLPEDQSRPLI
                     KQALDLGINFLDTANVYSDGHSEEIVGRALKDFVKRDDVVLATKVYSRMRKGPNGAGL
                     SRKAILSEVDHSLRRLGTDHIDLYQIHRWDPITPIEETLDALHDVVKAGKARYLGASS
                     MAAWRFTKALYTSRLRGWSEFVSMQNHLNLLYREEEREMLPLCLDQRVAVLPWSPLAR
                     GRLARDPE"
BASE COUNT          698 a         1500 c         1865 g          561 t
ORIGIN      
        1 tgggccgggc gcgcggcgac gccggcgggg ggcagcgcgc ggcggccgag gaagccgaga
       61 agatccgtgc ggcgtcgcgg gtggagaggg aggcggtcaa gcaggccgcg gaggtcgagg
      121 gcaaggaggc ggcgcgcaag cgcagggccg agctcgacga ggagctgcgg gcgcggcgca
      181 ccgagctggc gcggcgcgag gacgggctgg cgcagcgcga gcgcgaggtc gagaagaccc
      241 ggcgcgaggc ggagcgtcgc gcggccgagc tcgacaagaa agagaagagc atcgacggca
      301 agatcaagca ggcggactcg gcgatcgaga aggccgaggc ggcgcagggc gaggcgcgca
      361 agaagctcga ggagatcgcg gggctgtccg aggagcaggc gcgcaagaag ctcgaggacg
      421 aggtccgcgg ccaggcgctg accagcgcgg cggcggagat caagcggatc gaggacgacg
      481 cgcacaagga ggcgacggcg cgggccaaga cgatcgtcgc gacggcgatc cagcggttcg
      541 cgagcgagtt cgtgcacgag cggacggtgg cggtggtgcc gctgccgagc gatgacctga
      601 agggccggct gatcggccgc gagggccgca acatccgcgc gctggaggcg gcgaccggga
      661 tcgacatgat catcgacgac acggcggagt cgatcacgat ctcgtgcttc aacccggtgc
      721 ggcgggagat cgcgcggctg gcgatctcgg cgctggtcgc cgacgggcgg atccacccga
      781 cgcggatcga ggaggtggtg cgcaaggcgg agaaggaggt cgagaagctc tgcaaggagg
      841 ccggcgagca ggcggtgttc gacctcgggc tgcaccgcgt gcatccggag ctggtcaagc
      901 tcctggggcg gctgaagttc cggcagtcgt acgcgcagaa cgtgctgatg cactcggtgg
      961 aggtcggcta cctgtcaggg ctgatcgcgg ccgagctcgg ggcgaacgtg aagctggcgc
     1021 ggcgcgcggg gctgttgcac gacatcggca aggcgatcga ccacgagcac gagggagcgc
     1081 acgccgaggt cggggcgcag ctggcgcgca agcatcagga atcgccgcgg gtgtgccagg
     1141 cgatcgcgtc gcaccacggc gacgagggcg gcgcgcagcc gcaggcgctg ctcgatcaca
     1201 tcgtcgcggc ggcgaactcg ctgtcggcgg cgcggccggg cgcgcggcgc gagcagctgg
     1261 cgtcgtacat caagcggctc gacgacctgg aggcgatctg caagcggttc ggcggggtcg
     1321 agcgcgcgta cgcgctgcag gcgggccgcg agatccgggt gatggtgatg aacgaccagg
     1381 tcgacgacgc gcaggcgatc gtgatgtcga aggagatcgc gcgggcgatc gagaacgagg
     1441 cgacgtatcc ggggcaggtg cgggtcgtgg tggtgcgcga gacgcgggcg tcggactacg
     1501 cgcggtgacg gcgagggtgc gtcaggtccg cggcacgcag tagcacgcgc cggacggcgc
     1561 gaactggaac gccccgcccg ggcagcgcgg acacgtgctg gagcgacgcg gtgcacacga
     1621 gcacggtgag cgaatgcgct acagaccgat cacgtggcgc agggtgggcc gatgcgaggt
     1681 ccccaccgac ccgctggccg ccgcggggag gggcacgacc ggcgccggag gactgcgagg
     1741 cggcgcgccg gcggcagagc gatcacacgc ggcgcatgga tcggcggcgc cgtcgggcgc
     1801 gtgatcgggc gtgcgatcgg gcgcgtgcgg gacctcggcg agcaaggtcg ccgcgatcac
     1861 ggcgccggcg gcccagcgcc ggggcgagcc ggcgcgcacg gcggatgtgg aacgcagcac
     1921 gcgcatcgtg gatcagcgct cgggacgccg cggatcggcc gggggctgct tcggcaggtg
     1981 catcgagagc tcggcctggt tggcgccgat gatgagctcg cgcagcgcga cgaacagcag
     2041 cgcgcgggtc tcggacagcg cccggaccca cggcgcgcgc gggctgccga tcgcgcgcag
     2101 ctcgcggcgg aggtcggacg cgctgtcgac cgtccgcagg ttgagctcca gcatgcggcg
     2161 atgctcctcg tggagcgccc gcagggcctg caggttggcc tccagcaggt cgcgtagctc
     2221 gagcgcctcg gcccgggtcg ccggcagcgc gaccggccgg gtgaccgtgc gcggacgtgt
     2281 gccggggctg ggatgcgagg agctcgtcga cgaccgaccc gaggtggctt gcgtgtgtgt
     2341 catgccaaca agatacgcac cggcccggac aacccttgtc acggcggacg agtccggata
     2401 tgctcgaaat cgcttgtatc gtcgcgggga tctcagccat gtggcgcgga cgctgcgcgt
     2461 gctcgacgag ctgcgcgggt tccggcgcgg ccgccggctg agcgagatcg cggcggcgct
     2521 cggcgcgagc gagcggacgg tccgccgcga cctggcggac ctgcgcagcg ccgggttcga
     2581 gatcgagagc ctggcgatcg acgggcgggc cgcggcgcgg ctggtcgagc gcacgtactc
     2641 gagcatcgcg atcacgcggc gcgagcgcta cacgctgctc gcgatcgcga gcctgttcga
     2701 cgtgctgcgc ggcacgccgc tgtgggagga cgtgtcgagc ctgcagcgca agctggagca
     2761 gcggatgacg cccgaggagc gcgccgagca cgcgtcgctc ggcgatcgct tcgcgtacgt
     2821 gcccgacggc gggaccaagg cgtacgaggg caaggaggac gtgctggacg cgctgctgac
     2881 cggcgtgctg tcgcgccggg tggtgcggtt cgcgtaccgc gacgcccgcg gccgcgcgcg
     2941 cggcgggcac ctcgcgccgt acacgatgct gctctacaag catgggctgt acgtcgtcgg
     3001 ctgccggctg cgcgagcccg gcgacggacg cgcgatcgcg ccggggacgc cggtcgcggt
     3061 gtgggcggtc gagcggttca ccgacgccga ggcgctgcgc aaggcgccgt tcaccgcgcc
     3121 gcccgggttc cggatcggcg agctgctcaa cggcgcgttc ggcatccacg tcaccggcga
     3181 cgcggcgccg caccgcgtgg tgatcgagtt ctccgccgag aaggcggcgt tcgtgcgcgc
     3241 gcgggtgtgg cacccgacgc agacgttcga ggagctggcc gaccaccggg tgcggctggc
     3301 gttcacgtgc gtgagcctga tgccggtggt gtcgtgggtg ctcgagtggg ggccgcccgc
     3361 gcgcgcgatc gagccgcccg agctggtcgc cgaggtgatc cgcgagctcg aggcggcgcg
     3421 ccgcgcgtac cctggcgacg tcccgcacgg tcggggcgac gccggcgacc gggccgcgga
     3481 gagccgtcag gtgggtgcgc gccacgccgc cgcgggcgag tcgtagaaga accgctcgcg
     3541 cgcgaccttg tcgccgtccc agatctgcat cgcgatctcg tcgatgacgc ggatccggcc
     3601 gtcggggtgc gtgaactcga acacccagtg gatcgcgacg cgatcgccgt cgatcgcgac
     3661 gaactccggg cgatggctga cgatccgggc gacctgcgcg gtgaccttgc gctcgcgctc
     3721 gaccaggtgg gcgaggccgc ggcgcggctc ggcggcgttc tcctgcatgc tggcatcgtc
     3781 ggtgtagaac cgggcgatcg cctcggcgtg gtctccgttg accacggcgt cgatcagctc
     3841 ctggacgcgc tcgcgggtgg gcatgcgcgg accgtagcgc cggcgctgcg ccatggctcc
     3901 cgggcggccg acacagatcg cgccggccgc ggtagaacac cggccatgga atacaccaag
     3961 ctcggcgcca ccggcctgga ggtctcgcgg atctgcctgg gctgcatgtc gttcggcgcg
     4021 cccgatcgcg gcaaccacac ctggacgctg cccgaggacc agagccggcc gctgatcaag
     4081 caggcgctcg acctcggcat caacttcctc gacaccgcca acgtgtactc cgacggtcac
     4141 tccgaggaga tcgtcgggcg cgcgctcaag gacttcgtca agcgcgacga cgtcgtgctc
     4201 gcgaccaagg tgtacagccg gatgcgcaag ggccccaacg gcgccgggct gtcgcgcaag
     4261 gcgatcctgt cggaggtcga tcacagcctg cgccggctcg gcaccgatca catcgacctg
     4321 taccagatcc accgctggga tccgatcacg ccgatcgagg agacgctcga tgcgctgcac
     4381 gacgtggtca aggcgggcaa ggcgcgctac ctcggcgcgt cgtcgatggc ggcgtggcgg
     4441 ttcaccaagg cgctgtacac gtcgcggctg cgcgggtgga gcgagttcgt cagcatgcag
     4501 aaccacctca acctgctgta ccgcgaggag gagcgcgaga tgctaccgct gtgcctcgac
     4561 cagcgcgtcg cggtgctgcc gtggagcccg ctcgcgcgcg gccggctggc gcgcgacccc
     4621 gagg
//
LOCUS       DASVOR010000629         5221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_414523,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000629 DASVOR010000000
VERSION     DASVOR010000629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5221
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414523"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2136)
                     /locus_tag="VF516_12480"
     CDS             complement(<1..2136)
                     /locus_tag="VF516_12480"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8108538.1"
                     /translation="MVAGAAAGVLIWRSLRSPVSACEREVRTGDHRAAVGLCLRSYRD
                     TGDERALYWAAKADLYLGDLEGAERLVHRLSPPLDGDARGLLSYLALRQRRAREAQLQ
                     AILASAAHIAAGDRPGLASDAVLMSQAALLAGDFPPALRAADLAITLAQPLGDSHTAN
                     NGYIARADTLRQMGDIQGAGDTLATAAERATMPCDRTWTRLRRATCSIDLGRDELAMA
                     LLAETARENERCKSRDVSVQVASNQAWLLRKRDPARALAKLDELARWDDEGADALLLR
                     GYIAADRGALDDAENFLTRAGTTDDESAEWSWEIARVRGELLEQRGGELARLLAEYEY
                     RRSAAMVAALRAGAPAGSAFLVSAHRWPYDELIALLARERRWRDALAVVLDLDASDML
                     RATAVERAGSRRDTPDVDAAVAHPVVTPPPSVEAVLSAWSDRDLVIVLAQSTKQIGPG
                     KERVYRLHVSKGQVTGDDVGDAQTARAWAKALFEDPGNRDAARALGRMMVPPGSSDRA
                     LHVLAIGALGKVPLAALRDDDGSLLAGKRPLVRVLALRAARPESRGAGSPVVIADPRG
                     DLPGAAAEGSVVARAIGAEARVSGSRSARRASLAELWAGHDAAVLHIAGHVISLGRQR
                     VLPLVDGEVDPAEIVQHGLAPRIAVVASCGSAAATDEEGWGSIAAALLEAGTAAVVAT
                     DRRVDDGATLEMMRDFYAQPDWRADPARAL"
     gene            2601..2930
                     /locus_tag="VF516_12485"
     CDS             2601..2930
                     /locus_tag="VF516_12485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108539.1"
                     /translation="MSYQYFTIPISSVPDAPPYTVWFATWSADQCSFVWSTTRPVAGA
                     AGTTPTYVCGATDCAAPAGATVIIQNSAKTLPPPPVAVSSTASETEFQTALKQQLINT
                     LDGLRSL"
     gene            3354..>5221
                     /locus_tag="VF516_12490"
     CDS             3354..>5221
                     /locus_tag="VF516_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8108540.1"
                     /translation="MSKHIILFLAANPLGTTEFGPGQEARTIQKELERSGQRDRFELV
                     TRWAVQPLDLIRELRKLKPTVVHFSGQGASSGQQPGAGTRRDIAAEFGRPDGVADSDH
                     LGGLFLQGPDGHPQIVTAVALQETFGAAGTSVKLVVLNACYSDIHVEALLVHIDCVVA
                     MGGSLRDDATKAFAIGFYGGLGERQSVAAAYVQGCAAISLTGPSDCERPRLAVRAGID
                     ASQVVLADTRAVDRRRPDPAYPDVEVELLSKRVEDDRVRKDKLRGAEIAADHVDREIL
                     ELRRQLRKGGRLLAGETLSDGRYLLVRVVGRGGFAVVWEAHDSAAHQRVAIKVLHANL
                     GGDPLRRERFFRGAHARMKLVHPAVVRVLEPTREDGGFYYFVMEFVPGGNLREAVLAQ
                     HVNREDILPLILQVGDALALAHKRGMVHRDVKPANILLDEHGSARLTDFDLVNAQDTT
                     GGTRTGALGTVVYAAPECLEKPQEATARADVYGLGMTAIFCLSGRELSLATFRNPEPT
                     IARLKCASRVRNVLRQAVAWEPDKRFADASAMVAELRNAWNLAPERRKQPRKVTTERG
                     RWWMYAGAGVTAAAAVALVVLLPPPPALALNVEVKHTALFRGDEASAEDIAHITVS"
BASE COUNT          868 a         1831 c         1727 g          795 t
ORIGIN      
        1 cagcgcccgc gccggatcgg cccgccagtc gggctgggcg tagaagtcgc gcatcatctc
       61 cagcgtggcg ccgtcgtcga cccgccggtc ggtcgcgacc accgccgccg tgccggcctc
      121 cagcagcgcc gcggcgatcg agccccagcc ctcctcgtcg gtcgccgcgg ccgagccgca
      181 gctggccacg accgcgatcc gcggggccag gccgtgctga acgatctcgg ccggatcgac
      241 ctcgccgtcg accagcggca agacgcgctg gcggcccagc gagatcacgt ggccggcgat
      301 gtgaagcacg gcggcgtcat gccccgccca tagctccgcc aggctcgcgc ggcgcgcgct
      361 ccgcgatccc gacacccggg cctcggcgcc gatcgcccgt gcaacgacgc ttccctcggc
      421 cgcagcgccg ggaaggtcgc cgcgcggatc ggcgatcacg acgggcgaac cggcaccgcg
      481 agactccggc cgcgccgcgc gcagcgccag cactcgcacc agcggacgct taccggcgag
      541 caacgaacca tcgtcgtcgc gcagcgcggc cagcggcacc ttgcccagcg caccgatcgc
      601 cagcacgtgc agcgcgcggt cgctggatcc cggcgggacc atcatccttc ccagcgcgcg
      661 cgccgcgtcg cgattccccg gatcctcgaa cagcgccttc gcccacgcgc gcgcggtctg
      721 cgcatccccc acgtcgtcgc ccgtcacctg gcccttgctc acgtgcaatc gatacacgcg
      781 ttccttcccc gggccgatct gcttggtcga ttgcgccagc acgatgacca gatcccgatc
      841 gctccatgcc gacagcacgg cctccacgct gggcggtggg gtcacgacgg ggtgtgcgac
      901 cgccgcatcg acgtccggcg tgtctcgccg ggaacccgcg cgctcgaccg cggtcgcgcg
      961 cagcatgtcg ctcgcatcga gatcgagcac gacggcgagc gcgtcgcgcc agcgccgctc
     1021 gcgggcgagc agcgcgatca gctcgtcgta cggccagcga tgcgccgaca ccaggaatgc
     1081 cgacccggcc ggggcaccgg cgcggagcgc cgccaccatc gccgcggacc gccggtactc
     1141 gtactccgcg agcagacgag cgagctcacc gccccgctgt tccagcagct ccccccggac
     1201 gcgcgcgatc tcccaggacc actcggccga ttcgtcatcc gtcgtcccgg cccgcgtgag
     1261 gaaattttcg gcgtcatcga gcgcaccgcg gtcggcggcg atgtacccgc gcagcagcaa
     1321 cgcgtccgcg ccctcgtcat cccacctggc gagctcgtcg agcttggcga gcgcgcgggc
     1381 cggatcgcgc ttgcgcagca gccacgcctg gttggacgcg acctgcacgc tgacgtcgcg
     1441 cgacttgcag cgctcgtttt cgcgggctgt ttccgccagc agcgccatcg ccagctcatc
     1501 gcgccccagg tcgatcgagc acgtcgcgcg cctcaggcgg gtccacgtcc ggtcgcacgg
     1561 catcgtcgcg cgctccgccg ccgtggccag cgtgtcaccg gccccctgga tatcccccat
     1621 ctggcgaagc gtgtcggcgc gcgcgatgta tccattgttc gcggtgtgcg agtcgcccag
     1681 cggctgcgcc agcgtgatcg ccaggtcggc cgcgcgcagc gcggggggaa agtctcccgc
     1741 gagcagtgcg gcctgcgaca tcaagaccgc gtcgcttgcc aggcctgggc ggtctccggc
     1801 ggcgatgtgc gccgcgctgg cgaggatggc ctgcagctgg gcctcgcggg cgcggcgctg
     1861 acgcaacgcg aggtagctca gcagcccgcg cgcgtcaccg tcgagcgggg gggacaggcg
     1921 atggacgagc cgctcggccc cctcgaggtc tcccaggtac aggtccgcct tcgcggccca
     1981 gtacagcgcg cgctcatcgc cggtgtcccg gtagctccgg aggcacagcc cgacggcggc
     2041 gcggtggtcc ccggtccgga cttcgcgctc gcacgcggac accggcgacc gcagcgagcg
     2101 ccagatcagc actcccgcgg ccgctcccgc gaccaacccc gcagcgacca tcaggagcgc
     2161 cacgcgacct cgccttgcat gccgtcgaac gtgccgagcc atctccaggt ccgcgggtac
     2221 cagctactac gtccgcgtca ggataccatg ggcgacgaag acgtatgtca ggcggcctgg
     2281 accgcatcag ccaacgcctc gcacatcgcg atcaacggtc gcgcttcaca ccggactccc
     2341 gcgcggcaac gccgcggcca ccacgcggcg cggccggccg tggcgaggtg ggctcgtgca
     2401 gccagtttca cgacagccgc tcccctcaac aattcctggc ggatagcaca acagcttcag
     2461 ggaagtcgcg gaccgcagcg ttgtgctgtc ggatttcgaa tcgtcggtcg ccataaggcc
     2521 atcgtgggcg atgagtgtgg ctcctaggac tgccatctcc cgcctggact tgcagttcca
     2581 acgataggag gtctcctgcc atgagctacc agtacttcac catcccgatc agcagcgtcc
     2641 ccgatgcccc accgtacacc gtgtggttcg ccacctggtc ggccgaccag tgcagcttcg
     2701 tctggtccac gacgcggccg gttgcgggag cggcgggcac gacgcccacg tacgtatgcg
     2761 gtgccaccga ctgcgccgcg cccgccggcg cgaccgtcat catccagaac agcgccaaga
     2821 ccctcccccc gccgccggta gccgtctcca gcaccgccag cgagaccgag ttccagaccg
     2881 cgctcaagca gcagctcatc aacacgctcg acggactgag atcgctgtag cccgcgcgcg
     2941 gctccgccgg ggcgcgtacg ctgtctcgcg tgccccggcg cgataggatg cccgcccgca
     3001 ttcgaggacg tgcatgggac cgtcgtcgga tccagtgatc gatgtggcgc gcccgccctc
     3061 ggatccatcg gacgaggtcc ggcgcaagcc atccgacgtg tcggagcgcg agctacagga
     3121 tgcgctccgc gcatgtgcgt ggaacctcaa ggccgccgcc gaccgactcc ggatcctgcg
     3181 gccgtagatc tacgacctga ttgagcacac cccgacatcc gcaccgcctg cgacctcggt
     3241 gccgatgaga tcgcacttat cgatcggtcg gccggaacgc gatgtcgtat cgtcgctggc
     3301 ctcgccgcct gattgggacc agctaaggca tgcgctcgtg ctggaggtcg tcgttgagca
     3361 agcacatcat cctgttcctt gccgccaacc cgcttggcac cacggagttc gggcccggcc
     3421 aagaagcgcg cacgattcag aaggagcttg agcgaagcgg gcagcgggac cggttcgagc
     3481 tcgtaacgcg atgggccgtg cagccgctgg acctgatccg cgagctacgc aagctcaagc
     3541 caacggtggt gcacttcagc ggacaggggg caagcagtgg acaacaaccg ggcgctggaa
     3601 cacgccggga tatcgcggcc gagtttggcc gccccgatgg cgtggccgac agcgatcatc
     3661 tgggcggcct gttcctccag gggccagacg ggcatccgca gatcgttacg gcagtggcgc
     3721 ttcaggagac gttcggggcg gctgggacct ccgtgaagct cgttgtcctc aatgcttgct
     3781 acagcgatat acacgttgag gcgctgctcg ttcatattga ctgcgtcgta gccatgggtg
     3841 gctcccttcg tgacgatgcg acgaaggcgt tcgcaattgg tttctatggc gggcttgggg
     3901 aacggcagtc ggtagcggcg gcatacgtgc aagggtgtgc cgcgatcagc ctgacgggac
     3961 catcggattg cgaaagacca cgactcgcag ttcgcgctgg tatcgatgcc agccaagtcg
     4021 ttttggctga tactagagca gtcgatcgga gaaggcccga ccctgcctat ccagacgttg
     4081 aggtagagct actgagcaag cgggttgagg atgaccgcgt acggaaggac aagctgcgtg
     4141 gtgccgaaat agccgcggat cacgtcgatc gagagatcct cgagctgcgc agacagctgc
     4201 ggaaaggagg tcgactcctc gctggggaaa ccctaagcga cggccggtac ctgctggtgc
     4261 gtgttgtggg tcggggtggc tttgcagtgg tctgggaggc gcatgactcc gcggcgcatc
     4321 agcgcgtagc aatcaaggtg ttgcacgcga atcttggcgg tgacccgctt cggcgcgagc
     4381 gcttcttccg cggcgcgcac gcgaggatga agttggtgca tcccgcggtg gtgcgcgtcc
     4441 ttgagcccac cagagaggac ggagggttct actacttcgt catggaattc gtacccggcg
     4501 gcaacctgcg ggaggccgtg ctcgcgcagc acgtgaacag ggaggacata ttaccgctca
     4561 tccttcaggt cggcgacgcg cttgccctgg ctcacaaaag gggcatggtc caccgcgacg
     4621 tcaaaccagc aaacattctt ctcgacgaac atggtagtgc aaggctcact gacttcgacc
     4681 tcgtcaacgc gcaagacacg actggtggaa ctcgcaccgg cgcactcggg acagtcgtgt
     4741 atgcggcacc ggagtgcctt gagaagcctc aggaggctac cgcacgagcg gacgtttacg
     4801 gattagggat gacggcgatc ttttgcctct caggccgaga gctctctctg gcgacgttcc
     4861 gcaacccgga gccaacaatt gccaggctga aatgcgcgag ccgggttcga aacgtgctcc
     4921 ggcaagcagt ggcatgggaa ccagacaagc ggtttgccga cgcatctgcc atggttgccg
     4981 aactgcgcaa tgcatggaat ctcgcgcctg agcggaggaa gcaaccgagg aaagtcacga
     5041 ccgagcgcgg gagatggtgg atgtacgcgg gagcaggagt gacggcagcg gcagcggtgg
     5101 cacttgttgt gctgctcccg ccgccacccg cgctcgcgtt gaacgttgag gtcaaacaca
     5161 ccgcgctttt ccgcggcgat gaagccagcg ccgaagacat cgcacacatc accgtatcca
     5221 g
//
LOCUS       DASVOR010000630         2997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_502828,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000630 DASVOR010000000
VERSION     DASVOR010000630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502828"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..2111
                     /locus_tag="VF516_12495"
     CDS             402..2111
                     /locus_tag="VF516_12495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8108541.1"
                     /translation="MRDVDPTRPWTRLYPDWVPGELPLPTESMADVLEATARAAGEAP
                     AIHYFDATISYARLDDLASRFATLLASWGIGHGDRVALYLQNVPQYIVALYGIWKRGA
                     IAVPLNPMFKDKELAYHLEDSGARVLVALESLHAAFARHAAPGSPIERVVTTSELDML
                     GSEPPAAVFAASKKAAPAGTVDLLAALDRTTADPAARARVAPGDLASLGYTSGTTGQP
                     KGAMSSHLNIAYNANVYRVWLQLDARDVILGVAPLFHITGMVAHIAVAAVARCPVVLG
                     YRFDAAQTVALMTRWKPTMTVASITVYLALMNHPSTTREALASLTKALSGGAPVAPGI
                     ADRFEREFGVYIHNVYGLTESNSPTHAVPLGARAPVDPVTGALSVGLPIPGCDVKLVD
                     LDDPTKQVGPGEPGEIADRGPMIVSGYWNKPDANARAFHDGFFLTGDVATRTEDGYFF
                     VVDRKKDMINVSGYKVWPREVEDTLYLHPAVREAAVIGVPDDYRGETVKAFVALKPGS
                     SAAPDEIIEFCKSKIAAYKYPRQVEIVDEIPKTVTGKFLRRALRDQETEHRQDGAPRP
                     SRT"
     gene            2250..>2997
                     /locus_tag="VF516_12500"
     CDS             2250..>2997
                     /locus_tag="VF516_12500"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8108542.1"
                     /translation="MSTSFHLVVTHLVSIVTVVMTTVLISQVVVQHRAPGTTLAWLLA
                     IILIPYAGVPLYLVFGSHKLRRRGGKSQLYAPRPVPAVRGTIAGMLCASGAPPPRPGN
                     EVVVHTTGEQAYAAVIDALEAAARTIHVSTLILGDDPVGHAILAVLERKARAGVEVRL
                     LLDSLYRYRSNRRRLARLVAAGGEMAWFMPVWHVPFISRVRANLRLHRKILVTDGTTA
                     IIGGMNLAHEYMGPTPSPGRWRDLAARVRGP"
BASE COUNT          432 a         1084 c         1037 g          444 t
ORIGIN      
        1 tccggcacgc tcgtcgagct cgccggcggc ggcgcgtacg gcgtcgtgct cgtgacccgg
       61 accggcgcga gcgccgtcgg gacaccgggg cgggtcgtca gcgccgcccc ggccgcacag
      121 actccgaaca gaacgatcgc cactgccaga tacgtatcca ccttcacacg caactcccgt
      181 ctcacgaccc gcgaccaccg gcgtgatgca cgcttgatgc cgcgcgtcaa cgagctcgcg
      241 ggaccgaaac gcgccgaggt tgcatggtcg tcaccggcgg ctcgtcgcgg gtctgtggtg
      301 tcggcgcgcg gtgggcgaga tcgtgcgggt caccgtggca catccagctc gcgtgacacc
      361 ggtggcgggc gcgcagccag acgtggtgaa ctgacgccgc catgcgcgac gtcgacccca
      421 cgaggccgtg gacccggctc tacccggact gggtccccgg cgagctgccg ctccccaccg
      481 agagcatggc cgacgtcctc gaggccaccg cccgcgccgc gggtgaggcg ccggcgatcc
      541 actacttcga cgcgacgatc tcgtacgcgc ggctcgacga tctcgcgtcg cggttcgcga
      601 ccctgctcgc gtcgtggggc atcgggcacg gcgatcgggt cgcgctctac ctgcagaacg
      661 tgccgcagta catcgtcgcg ctgtacggca tctggaagcg cggcgcgatc gcggtgccgc
      721 tcaacccgat gttcaaggac aaggagctcg cctaccacct cgaggactcc ggcgcgcgcg
      781 tgctcgtggc gctcgagtcg ctccacgcgg cgttcgcccg gcacgcagcg cccgggtcgc
      841 cgatcgagcg ggtcgtgacc acgagcgagc tcgacatgct cggcagcgag ccgcccgccg
      901 cggtgttcgc ggcgagcaag aaggccgcgc cggcggggac cgtcgatctg ctcgcggcgc
      961 tcgaccggac gaccgccgat ccggcggcgc gcgctcgggt cgcgccgggc gacctcgcgt
     1021 cgctcggcta cacgtcgggc accaccggcc agcccaaggg cgcgatgagc tcgcacctca
     1081 acatcgccta caacgcgaac gtctaccggg tgtggctcca gctcgacgcg cgcgacgtca
     1141 tcctcggcgt cgcgccgctg ttccacatca ccggcatggt ggcgcacatc gcggtcgccg
     1201 cggtggcgcg ctgcccggtg gtgctcgggt accggttcga cgcggcccag accgtggcgc
     1261 tcatgacgcg ctggaagccg acgatgaccg tcgcctcgat cacggtgtac ctcgcgctga
     1321 tgaaccatcc gtcgaccacg cgcgaggcgc tggcgtcgct gaccaaggcg ctctcgggcg
     1381 gcgcgccggt cgcgccgggc atcgccgatc gcttcgagcg cgaattcggc gtctacatcc
     1441 acaacgtcta cgggctcacc gagtcgaact cgccgaccca cgcggtgccg ctcggcgcgc
     1501 gcgcgccggt cgatccggtc accggcgcgc tctcggtcgg gctgccgatc ccgggctgcg
     1561 acgtgaagct cgtcgacctc gatgacccca cgaagcaggt cgggccgggc gagcccggcg
     1621 agatcgccga tcgcgggccg atgatcgtgt ccggctactg gaacaagccg gacgcgaacg
     1681 cgcgcgcgtt ccacgacggc ttcttcctca ccggcgacgt cgcgacgcgc accgaggacg
     1741 gttacttctt cgtcgtcgat cgcaagaagg acatgatcaa cgtctccggt tacaaggtct
     1801 ggccgcgcga ggtcgaggac acgctgtacc tccaccccgc cgtgcgcgag gcggcggtga
     1861 tcggggtgcc cgacgactac cgcggtgaga ccgtgaaggc gttcgtggcg ctcaagccgg
     1921 gcagctcggc ggcgcccgac gagatcatcg agttctgcaa gtcgaagatc gcggcctaca
     1981 agtatccgcg gcaggtcgag atcgtcgacg agattcccaa gaccgtgacc ggcaagttcc
     2041 tgcggcgcgc cctccgcgac caggagaccg agcatcgcca ggatggtgca ccacggccgt
     2101 cacggacgta gtcactgata cctcactttc aatcccggac agctcgcgct catacgatat
     2161 tgtcgcgtct tgtggtcgga ggcatgcggc gcgcctgcat gcgctcgccg gcagacggtc
     2221 ggtccgccat cggctaagct caccgcgcct tgtcgacgtc gttccacctg gtggtcaccc
     2281 acctcgtctc gatcgtcacg gtcgtgatga ccacggtgct gatctcgcag gtcgtggtcc
     2341 agcaccgcgc gccgggaacc acgctggcgt ggctgctcgc gatcatcctg atcccgtacg
     2401 ccggcgtgcc gctgtacctg gtgttcggca gccacaagct gcgcaggcgc ggtggcaagt
     2461 cacagctcta cgcgcccagg ccggtgccgg ccgtgcgcgg aacgatcgcc gggatgctgt
     2521 gcgcctccgg tgcgccgccg ccgcggcccg ggaacgaggt cgtcgtgcac accaccggcg
     2581 agcaggccta cgcggcggtg atcgatgcgc tcgaggcggc cgcgcggacg atccacgtct
     2641 cgaccttgat cctcggcgat gatccggtgg gccacgcgat cctcgcggtg ctcgagcgca
     2701 aggcccgggc cggcgtcgag gtccgcctgc tgctcgactc gctgtaccgc taccggagca
     2761 accggcggcg gctcgcccgc ctggtcgcgg ccggcggtga gatggcgtgg ttcatgccgg
     2821 tgtggcatgt cccgttcatc agccgggtcc gcgccaacct gcgcttgcac cgcaagatcc
     2881 tcgtcaccga cggcacgacc gcgatcatcg gcggcatgaa cctggcgcac gagtacatgg
     2941 ggccgacgcc gagcccgggg cggtggcgcg atctggcggc gcgtgtgcgc ggtccgg
//
LOCUS       DASVOR010000631         7100 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_6189,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000631 DASVOR010000000
VERSION     DASVOR010000631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6189"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1038
                     /locus_tag="VF516_12505"
     CDS             <1..1038
                     /locus_tag="VF516_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108543.1"
                     /translation="DGTWEVIDGVQRLSTLAHFGTTRPGVLRELGRHEPLTLTGLDRL
                     TEFNGRKLAELPPPIQLQFWRRALRVTALSDHGHPDVRRETFRRLNPGSVAASDHEVG
                     TSVGGGAGPPDRPGDRATVPIRNARLNSTLAATSILLDVASREVRVAAALEESTLTDR
                     QDQAAIRKAGAYLYAAAALERACREGLLAVIAEIDAHGVAPERLRVSLFALHCAAELD
                     AIGGGPRSLERVVRAAAMFETVFGAAHAPLGTVLPLDGRTPRAAHFATIWRVMGFPGP
                     ALPDPTCGLALDDLAEGRNDVAHGVRDAIAFGRSKAVADVVRLVGRVEQMVAHLFESA
                     DAYLDQRLFLR"
     gene            1065..1979
                     /locus_tag="VF516_12510"
     CDS             1065..1979
                     /locus_tag="VF516_12510"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEX8108544.1"
                     /translation="MLTVFGSINVDLTFALPHLPAAGETVLTSTYSQAVGGKGANQAA
                     AAARDGAATGFIGCVGDDGLGATARAALAELGVDVGGLETVPGTTGVAAIWIDGEGKN
                     QIAVASGANSALKADALVRRPLARGGFVVLQMETPAAEVAAVIAHARRAEARVILNLA
                     PALPLLRPALEQVDILVVNEHEAAVLCERLELALRNPADQASGLARHLGNTVIVTLGA
                     AGAIGARQDEVLRVAALAVNAVDTTGAGDCFVGVLASALARGASLHTAMQRAAIAGSL
                     ACTVVGAMPSFPGRAQIDAALLDTGHIP"
     gene            1976..2908
                     /locus_tag="VF516_12515"
     CDS             1976..2908
                     /locus_tag="VF516_12515"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEX8108545.1"
                     /translation="MTRTKILIDCDPGHDDAVAILFAARHLDLVGITTVHGNNTVEHT
                     TRNALALIELAGLDVPVAIGCADPLAQRRAPRAGTGLDGAVLPEPGRRPVDTHAIEFM
                     IETAHRHRGELVLAAIGPQTNVALALRREPRLKDWLREITIMGGSTGIGNVTPVAEFN
                     IHCDPEAAWAVFNSGAPLRMVGLNVTRCTGFDSADVARLKGSGRKVASVIADLMGFYL
                     ARVREWRGVDVAPMHDVCAIVPHVEDGLIDYMFTRVDIELAGAHTRGMTVCDLRQGRS
                     AVPGDRRAANATVAVDAKCRPLIEHVIATLLSYD"
     gene            2935..4023
                     /locus_tag="VF516_12520"
     CDS             2935..4023
                     /locus_tag="VF516_12520"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8108546.1"
                     /translation="MTRTFAMGDPQGPFDKLLEVLGRHGALDGDRLARDVVLVSIGDH
                     FDYDIHDWQTAGQEGLRFLRWLASHDPAQAVLLLGNHDTARVMELVAISDERFAAARA
                     FAWTLHGDPARDVRFLAEFPELQCADLTRRDFASFTVEQRALMMELLLAGRFNLARVG
                     ELTDGREALLTHAGVTTRELGMLGAPAEPRAIADALQDALARAIDRVRDDWTRGISTP
                     LSLEPLHIAGSPGEEGAGLLYHRPSNPDRRGADRAWQFAPGRPRRFDPRTLPLGLTQV
                     VGHTGHNRCLTELDGWATTAACGRKHGGIRTLRLAGQTVTYDLGVADPAPGVADLILI
                     DGELWRVPAAEVALLPLAGLCRAPNGSP"
     gene            4020..4895
                     /locus_tag="VF516_12525"
     CDS             4020..4895
                     /locus_tag="VF516_12525"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8108547.1"
                     /translation="MTSLRVVTLNLWGLEPPLDARLALAARQLRALAPDVVCLQEVRP
                     LGGRSGRTTADVLADALDMTAHYETAVAWEAAHDLPGGEEGLAVIARTLRDTRVLALP
                     EARPGEARILLSAAIDTAGGPIWVHTTHLHYRLDDGVAREHQVLAIDDAIRRFGRDND
                     SPPQILCGDFNAPADSDEIRFLRGLTTLGGRRTHFQDAWLRVHREPVSGDGPAEGITR
                     SSENRFPGSPRAVILARRIDYVFVTTRKKDGRGTVHDCRVVLTEREGAGEVSFCASDH
                     YGVCVDVQVAPIAAT"
     gene            4954..6423
                     /locus_tag="VF516_12530"
     CDS             4954..6423
                     /locus_tag="VF516_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108548.1"
                     /translation="MTIAVRCGRAAALTAIGCVLASAAARTALADPAADPDPAAALAS
                     AREALGFARAGQSVIHYRAQTAAEQNYQSDRSYPPFFSAMTVKEAWFDPASGVERAAL
                     ETTYPGSPASLQVVAGDAGRAFAMTKDGARPLPFSAVRARYLNPWAVIADWSAARDIR
                     SGGREPYRDYKRLVLIRTTPEGEQRLFLDVKTGFPVKLALVEKHYLWGQRSIEYVYSN
                     WTALAGVMVAASSFRLADGKTETSQTIGTVELVSRAAAPALALPAQPDRSPDALPGFL
                     QPLDVTAIQVSPNTYLLSNPGYTEAVTRVGDEVIVFDATQGEDRARKDAEAIARIFPG
                     AHKVTVVVTDLAWPHISGVRYWVASGATIVTHTAACEFLRRVIDRRWTVSPDLLERRR
                     ATVKLKLVGVDATYKLASGAVSLHPIDGIGTETALLGYVSADRFLWASDYIQTLSEPT
                     SYAAEVISAVRQHGLTPERAAAEHLPLTPWSQLEPLGQR"
BASE COUNT         1070 a         2479 c         2472 g         1079 t
ORIGIN      
        1 gacggcacct gggaggtgat cgacggtgtc cagcggctct cgacgctggc gcacttcggg
       61 acgaccaggc caggcgtgct gcgcgagctc ggcaggcacg agccgctcac gctcaccggc
      121 ctcgaccggc tgaccgagtt caacggccgg aagctcgcgg agctgccgcc gccgatccag
      181 ctccagttct ggaggcgcgc gctgcgcgtg accgcgctgt ccgaccacgg ccatccggac
      241 gtccggcgcg agacgttccg gcgcctgaac ccaggcagcg tcgcggcgag cgaccacgag
      301 gtcggcacca gcgtgggcgg cggcgcgggg ccgccggatc gcccgggcga tcgagcgacg
      361 gtcccgatca ggaacgcgcg cctgaacagc acgctggcgg cgaccagcat cctgctcgac
      421 gtcgcgtcgc gcgaggtccg cgtcgccgcc gcgctcgagg aatcgaccct gaccgatcgc
      481 caggaccagg ccgcgatccg caaggccggt gcctacctgt acgccgcggc tgcgctcgag
      541 cgcgcctgcc gcgagggcct gctggcggtc atcgccgaga tcgacgccca cggcgtggcg
      601 cccgagcgct tgcgggtcag cctgttcgcg ctccactgcg ccgcggagct ggacgcgatc
      661 ggtggcggcc cccgatcgct cgagcgcgtg gtgcgggccg ccgcgatgtt cgagaccgtg
      721 ttcggtgccg cgcatgcgcc gctgggaacg gtgctgccgc tcgacggcag gacgccgcgg
      781 gccgcgcact tcgcgacgat ctggcgcgtc atggggttcc ccggacccgc cctgcccgat
      841 cccacctgcg ggctcgcgct cgacgacctg gcagaaggcc ggaacgatgt cgcccatggc
      901 gtgcgggatg cgatcgcgtt cggacggtcc aaggcggtcg cggatgtcgt tcgcctcgtc
      961 ggtcgcgtcg agcagatggt cgcgcatctg ttcgagagcg ccgacgcata tctcgatcag
     1021 cgcctgttcc tccggtgacc acgccagccc agcacgatat gcccatgctg accgtcttcg
     1081 gctccatcaa tgtcgatctg accttcgcgt tgccgcatct gccggccgcc ggcgagacgg
     1141 tgctgacctc gacctacagc caggccgtcg gcggcaaggg cgcgaaccag gccgccgccg
     1201 cagcgcgcga cggcgcagcc accggtttca tcggttgcgt cggggatgac ggccttggcg
     1261 cgaccgcgcg cgcggcgctg gcggagctcg gcgtcgacgt cggcggcctg gagaccgtac
     1321 cgggaaccac gggagtcgcg gcgatctgga tcgatggcga agggaagaac cagatcgccg
     1381 tggcatccgg tgccaactcc gccctcaagg ccgacgcgct cgtgcggcgc cccctcgctc
     1441 gcggcggctt cgtggtgctg cagatggaga cacccgccgc cgaggtggcg gccgtcatcg
     1501 cgcatgcacg gcgagcagag gccagggtca ttctcaatct tgcgccggcg ctgcccctgc
     1561 tccgcccggc gctcgagcaa gtcgacatcc tggtcgtcaa cgagcacgaa gcggcggtgc
     1621 tgtgcgagcg cctggagctg gcactgcgca acccggcaga ccaggcatcc ggactggcgc
     1681 gccatctcgg caacaccgtc atcgtcacgc tcggcgctgc cggggcgatc ggggcgcgcc
     1741 aggacgaggt gctgcgggtc gcggcgcttg ccgtgaatgc cgtcgatacc acgggcgctg
     1801 gagattgctt tgtcggcgtg ctggcgtccg cgctggcgcg gggcgcgagc ctgcacaccg
     1861 ccatgcaacg cgcggccatc gccgggagcc tggcctgcac ggtggtcggg gcaatgccga
     1921 gctttcccgg ccgtgcacag atcgacgccg cgctgctcga caccgggcat atcccatgac
     1981 caggaccaag attctcatcg attgcgatcc gggccacgac gacgcggtgg ccatcctgtt
     2041 cgcggcacgg cacctggatc tggtgggcat cacgaccgtc cacggcaaca acacggtgga
     2101 gcacacgacg cgcaacgcgc tggcgctcat cgagctcgcc ggtctcgacg tgcccgtggc
     2161 gatcggttgc gccgatccgc tggcacagcg cagggccccc cgcgccggga ccggtctcga
     2221 cggcgccgtt ctgccggagc ccgggcgccg acccgtcgac acgcatgcca tcgagttcat
     2281 gatcgagact gcgcaccgcc accgcggcga gctggtgctg gctgcgatcg gcccgcagac
     2341 gaacgtggca ctggcgctca ggcgggagcc gcggctcaag gactggctgc gcgagatcac
     2401 gatcatgggc ggctcgacag ggatcggcaa cgtgaccccc gtcgccgaat tcaacattca
     2461 ctgcgatccc gaagccgcgt gggccgtgtt caacagcggc gccccccttc gcatggtggg
     2521 attgaacgtg acgcgctgca ccgggttcga ctcggccgat gtcgcgcgcc tcaagggctc
     2581 gggtcgcaag gtcgcgagcg tgatcgcaga tctgatgggc ttctatctcg cgcgcgtgcg
     2641 cgagtggcgc ggggtggatg tcgcaccgat gcacgatgtt tgtgccatcg tcccgcacgt
     2701 cgaagacggg ttgatcgatt acatgttcac ccgagtggat atcgagctcg ctggcgcgca
     2761 tacgcgcggg atgaccgtgt gcgatctgcg ccagggacga tccgccgtgc ccggagaccg
     2821 acgagcggcc aatgcgacgg tcgcagtcga tgcgaagtgt cggccgttga tcgagcacgt
     2881 catcgccacg ctgctgtcct atgactgaga gacgccgtgc gctaggatga tcgggtgacc
     2941 cgcacgttcg cgatggggga tccgcaaggg ccgttcgaca agctgctcga ggtcctgggc
     3001 cgtcacgggg cgctcgacgg cgaccggctg gcgcgcgacg tcgtgctggt atcgatcggc
     3061 gaccacttcg attacgacat tcacgactgg cagacggccg ggcaggaggg actgcggttc
     3121 ctgcgctggc tggcgagcca cgacccggcg caggccgtgc tgctgctcgg caaccacgac
     3181 actgcgcggg tcatggagct ggtggcgatc agcgacgagc ggttcgccgc ggcgcgagcc
     3241 ttcgcgtgga cgctccacgg cgatcccgcg cgcgatgtcc ggttcctcgc ggagttcccc
     3301 gagctgcagt gcgccgacct gacccgccgc gactttgcgt cgttcacggt cgagcagcgc
     3361 gcgctgatga tggagctctt gctcgccggg cggttcaacc tggcgcgggt cggcgagctg
     3421 acagacggcc gcgaggcgct gctcacccac gccggcgtga ccacccgcga gctgggcatg
     3481 ctgggcgcgc cggccgagcc gcgggcgatc gcggatgcgc tccaggatgc gctcgcccgt
     3541 gcgatcgatc gcgtacgcga cgactggacg cgtgggatct cgacgccgct gtcgctcgag
     3601 ccgctgcaca tcgcggggag cccgggcgag gagggtgccg ggctgctgta tcaccggccc
     3661 agcaaccccg atcgccgcgg cgccgaccgc gcgtggcagt tcgcgccggg ccggccgcgc
     3721 cggttcgatc cgcgaaccct gccgctcggc ctcacccagg tcgtcggcca caccggccac
     3781 aaccggtgcc tgaccgagct cgacggctgg gcgaccactg ccgcgtgcgg ccgcaagcac
     3841 ggcgggatcc ggacactgcg gctcgcgggc cagaccgtca cctacgatct cggcgtggcc
     3901 gatccggcgc ccggcgtggc cgacctgatc ctgatcgacg gcgagctgtg gcgggttcca
     3961 gccgccgagg tcgcgctgct cccgctcgcc gggctgtgcc gagcgccgaa cggatcccca
     4021 tgacgagcct gcgtgtcgtg accttgaacc tgtggggcct cgagccaccg ctcgatgcgc
     4081 gcctcgcgct cgcggcgcgc cagctccgtg cgctcgcccc cgacgtcgtg tgcctgcagg
     4141 aggtccgtcc gctcggcggc cggtccggcc gcaccaccgc cgatgtcctc gccgacgcgc
     4201 tcgacatgac ggcgcactac gagacggcgg tggcctggga ggcggcgcac gacctgccgg
     4261 gcggggagga agggctcgcc gtgatcgcgc gcaccctccg tgacacccgg gtgctcgcgt
     4321 tgcccgaggc gcggcccggc gaggcgcgga tcctgctgtc ggcggcgatc gacaccgcgg
     4381 gcggcccgat ctgggtgcac accacgcacc tgcactaccg gctcgacgac ggcgtcgcgc
     4441 gggagcacca ggtgctcgcg atcgacgacg cgatccgccg gttcggccgc gacaacgaca
     4501 gcccaccgca gatcctgtgc ggcgacttca atgccccggc cgacagcgac gagatccggt
     4561 tcctgcgcgg gctgaccacc ctgggcggcc gccgcaccca cttccaggac gcctggctgc
     4621 gcgtgcacag agagcccgtc tccggagacg gcccggccga gggcatcacg cggtcgagcg
     4681 agaaccgctt ccccgggtcg ccgcgcgcgg tcatcctcgc tcgccggatc gactacgtgt
     4741 tcgtcaccac gcgcaagaag gacggccgcg gcacggtcca cgattgtcgc gtggtgctga
     4801 ccgagcgcga gggcgctggc gaggtctcgt tctgtgccag cgaccactac ggcgtgtgcg
     4861 tcgacgtcca ggtcgcgccg atcgccgcga catgatctgc agctcccctg gcggatcgcg
     4921 tcgtgctacg ccaggggcat gatcggcggg ctcatgacca tcgctgtccg ttgtggacgc
     4981 gccgcggcgc tcaccgcgat cggttgcgtg ctcgcgtcgg cggccgcccg gaccgccctg
     5041 gccgatccgg cggcggatcc cgatccggcg gccgcgctgg cgagcgcgag ggaggcactc
     5101 ggcttcgcgc gagccgggca gtcggtgatc cattatcgtg cgcagaccgc agcggagcag
     5161 aactaccagt ccgatcgcag ctacccgccg ttcttctcgg cgatgaccgt gaaggaggcg
     5221 tggttcgatc cggccagcgg ggtcgagcgg gcagcgctgg agacgaccta tccgggcagc
     5281 cctgcctcgc ttcaggtggt ggccggcgat gctggacgcg cgttcgcgat gaccaaggac
     5341 ggggccaggc cgttgccgtt ctcggcggtg agagcgcgct acctgaatcc atgggcggtg
     5401 atcgccgact ggtccgcagc cagggacatc cggagcggag ggcgcgagcc gtaccgcgac
     5461 tacaagcggc tcgtcctgat ccggaccacg cccgagggcg agcagcggct gttcctcgac
     5521 gtcaagaccg gtttcccggt gaagctcgcg ctggtcgaga agcattatct ctggggacag
     5581 cgctccatcg agtacgtcta ctcgaactgg accgccctcg ccggcgtgat ggtggccgcc
     5641 tcgagcttcc gcctggctga cggcaagacc gagacgtcgc agaccatcgg gaccgtggag
     5701 ctcgtgtcgc gtgccgcggc gccagccctc gccctgccgg cgcagccgga ccgatcgccc
     5761 gacgcgctgc ccggattcct gcaaccgctc gatgtcacgg cgatccaggt cagcccgaac
     5821 acctacctgc tgtccaatcc tggctacacc gaagcggtga ccagggtcgg ggacgaggtg
     5881 atcgtcttcg acgccacgca aggcgaagac cgggccagga aggatgccga ggcgatcgcg
     5941 cggatctttc ccggcgctca caaggtgacc gtggtggtca ccgaccttgc ctggccccac
     6001 atctccggag tgcgctactg ggtggcaagc ggcgcgacga tcgtgacgca caccgcggcg
     6061 tgcgaattct tgcgcagggt gatcgatcgg cggtggaccg tatcacccga tctcctcgag
     6121 cggcggcgcg cgaccgtcaa gctcaagctc gtcggtgtgg acgcgaccta caagctcgcg
     6181 agcggcgcgg tcagcctgca cccgatcgat ggcatcggca ccgagaccgc cttgctcggc
     6241 tacgtgtcgg cggatcgctt tctgtgggca tcggattaca tccagacgct gagcgagccg
     6301 acttcctatg ccgccgaggt gatcagcgcg gtgcggcagc atggactgac tcccgagcgc
     6361 gccgccgccg agcacttgcc gctcacgccg tggagccagc tcgagccgct gggacagcga
     6421 taacgctgcc agcaaccagc gtactcagcc tcacctgcga cgccgatacc gtaagccagg
     6481 ggtgcgttgc gggtgtaacc tcgtgcgaga ggctcccacg gactgccatc ctgggctccg
     6541 agcacggctg ctccggattg aacattggat gttcccggat ccggagcatt cataagacgc
     6601 atctacgtca ctggtgccgg gatccgccgg tcgttatcct aatcgaaata ttacggcgga
     6661 ttagcactgc gacaccggtg atcacctcgg ggggttcagg cgtctcacag tagagctttg
     6721 cacgaatagc gattgtcgat tccagcggcc ttgcattagt attcttccga tctcaatcac
     6781 gagatcgagc aaacaggaga tatcaatgaa gaagatcccg atccgcgccg ccgagaccat
     6841 caagacgact gctgcactct actgcagctg ttgtgtgcag taacacacag cagcacgggc
     6901 cctgtaccct ccgataggag tacggggtcc gccccttgtt tccccgagaa ctcacacgta
     6961 gatgagccgg tacagcaccg acagccacgt attcgtacag ccgttctccc gacaacccga
     7021 cggagacgag gttgtcatcg gcgtgcagga ctccttcatt gcattgcccg gcgcggccgt
     7081 ggatctcctc gactggctcg
//
LOCUS       DASVOR010000632         5633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_152132,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000632 DASVOR010000000
VERSION     DASVOR010000632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5633
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152132"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3374)
                     /gene="secA"
                     /locus_tag="VF516_12535"
     CDS             complement(<1..3374)
                     /gene="secA"
                     /locus_tag="VF516_12535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEX8108549.1"
                     /translation="MGIFQKIFGSRNQRELRKLQPIVDRINHLEPTIKAKSDDELRAM
                     TGEFKRRLDKGATLDEILPDAFALVREASVRRTGMRHYDVQMIGGIVLHQGKIAEMRT
                     GEGKTLVATLPTYLNALTGQGVHVVTVNDYLAKRDAEWMGRIHSVLGLTTGVIVHGLD
                     DIARQNNYNCDITYGQNNEFGFDYLRDNMKESPDRMVQRGLYYAVVDEVDSILIDEAR
                     TPLIISGQGEASADLYEKIDRLIPRLKREVDYTVDEKAHSAMLTDDGVERIEKLLGVD
                     NLYAASNIVLVHHVNQALRAHTLYKRNVNYLVENNKVIIISEDTGRKMEGRRWSDGLH
                     QAIEAKEGVTVEEENQTLATVTFQNYFRLYKKLAGMTGTADTEAQEFHQIYKLDVSVI
                     PTNRPMIRKDHPDLVYKNEAGKFRAVVADVEDCHQRGQPVLVGTVSVEKSEVVANLLK
                     AKGLPFNVLNAKQHQREAAIVAQAGRKAAITIATNMAGRGTDILLGGNPEAMAKEALA
                     EERAKLAETADGAQPVAPAGDDPPEPYRGSIEPRVDEEARYQELLANFKAQCDAEREE
                     VLAAGGLKIVGTERHESRRIDNQLRGRAGRQGDPGGSRFYLSLQDDLLRIFGLDKMTG
                     LMERLGLEEDVPIESPMVTRSIENAQKKVEGRNFDQRKNVLEYDDVMNQQRKTIYALR
                     RQILEGRYHREPTEDEAKQGIVPEPVTSSGDWTIESLTPELKQYLGGQTAVHRLVPAG
                     GADHGVPPFHPVTALKDSTLTLPDQPRLTTGDAVVYRAGGEPAIDRLVDGETYYVIGE
                     TPGPFALARSKDDVKKGLAIAIGPRRGLVDLVKDAVKERDELVARGEPLTERLQRLVG
                     TRPGWRVLRFEIWRQFGVLLNLEKRYDAERDELLDSITRDVAASLIQQRERMYDLCDT
                     RMAAVIERVLSPDVPDDEWDWDELEDALQEQFATQFELSPGTPDEVAGQMWPVIERRL
                     AEREKELSRAWLMHFQRQFSLTEIDEQWIDHLKTMDALREGIGLQGYGQKDPKKEYKK
                     IGFDMFRDMMDRIGANTVTKLFRVQIQREEEQAQVPQLKHKERHLEEHGAANKSEDEV
                     AAAAEQDSSARQVAAQNKRAARGRPAAAARGGDGDGV"
     gene            3772..4131
                     /locus_tag="VF516_12540"
     CDS             3772..4131
                     /locus_tag="VF516_12540"
                     /inference="COORDINATES: protein motif:HMM:NF020846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease pro-enzyme activation domain-containing
                     protein"
                     /protein_id="HEX8108550.1"
                     /translation="MPRAASSLIASSAAALALGAAPAAAAPLAPAATDLDPIPTYHHF
                     LSMRELADLFAPSQGDIATITRFPSSFRITVNEMFADRLLIKATGGIPGYEPGAGLDV
                     LDVANFSTAVGRELRGH"
     gene            4336..5598
                     /locus_tag="VF516_12545"
     CDS             4336..5598
                     /locus_tag="VF516_12545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8108551.1"
                     /translation="MKIDRDAPPAGRRWSRLVAALVVLAVLVGAGYAFGYRKLRASLA
                     TPEVKIGEVALVSPVQSQVQLTATGYVVALVYAKVASKVPGRIAEIFVEEGQTIEKGS
                     RVARLEDVDFKTQLATARARAASARARIAVAKASLVEIKVQIDRERPMVDKGVTAKAT
                     LDDLEARRGSAEAAVRAAEAEAMATDADVKALEIQLGSYEIITPISGTVVDKLVKVGE
                     GVSPGFGTPGVIEVIDLTSLVVEVDVPEGRLSQVVVDAPCEIVLDAYPTRRYRGAVKE
                     IGRRVNRSKATVPVKIRFVDLADGRPVPGGTSNSGSGEVGGPGAGKPEILPDMAARVS
                     FLTSTIDPATLDTPPRLTVPAGAVVQRNGRDVVFVVDEGEVRMTPVALGAASGDGREL
                     VTKLPPGTKVVIDPPADLGDGQKVKENK"
BASE COUNT          752 a         1949 c         1956 g          976 t
ORIGIN      
        1 accccgtcgc cgtcgccgcc gcgcgcagct gccgccgggc gcccgcgcgc agcccgcttg
       61 ttctgtgctg ccacctggcg cgccgaactg tcctgctcgg ccgccgcggc gacctcgtcc
      121 tccgacttgt tggcggcgcc gtgctcctcc aggtggcgct ccttgtgctt gagctgcggc
      181 acctgggcct gctcttcctc gcgctggatc tggacccgga acagcttggt gaccgtgttg
      241 gccccgatcc ggtccatcat gtcgcggaac atgtcgaatc cgatcttctt gtactccttc
      301 ttcggatcct tctggccgta gccctgcagg ccgatgccct cgcgcagcgc gtccatcgtc
      361 ttgaggtggt cgatccactg ctcgtcgatc tcggtcagcg agaactgccg ctggaagtgc
      421 atcagccagg cgcgcgacag ctccttctcg cgctcggcga gccggcgctc gatcaccggc
      481 cacatctggc cggcgacctc gtcgggcgta cccggcgaga gctcgaactg ggtcgcgaac
      541 tgctcctgga gcgcgtcctc cagctcgtcc cagtcccact cgtcgtcggg gacgtcgggc
      601 gacagcacgc gctcgatcac cgcggccatc cgcgtgtcgc acagatcgta catccgctcg
      661 cgctgctgga tcagcgacgc ggcgacgtcc cgggtgatcg agtcgagcag ctcgtcgcgc
      721 tcggcgtcgt agcgcttctc gaggttgagc agcacgccga actggcgcca gatctcgaac
      781 cggagcacgc gccagcccgg ccgggtgccg accagccgct gcagccgctc ggtcagcggc
      841 tcgccgcgcg cgaccagctc gtcgcgctcc ttgaccgcgt ccttgaccag atcgaccagg
      901 ccgcgccgcg ggccgatcgc gatcgccagg cccttcttga cgtcgtcctt gctgcgcgcg
      961 agcgcgaacg ggccgggcgt ctcgccgatc acgtagtagg tctcgccgtc gaccagccgg
     1021 tcgatcgcgg gctcgccgcc cgcgcggtag accaccgcgt cgccggtcgt cagcctgggc
     1081 tgatcgggca gcgtgagcgt gctgtccttg agcgcggtca ccgggtggaa cggcgggacg
     1141 ccgtggtccg cgccgccggc cgggaccagc cggtggaccg cggtctggcc gcccaggtac
     1201 tgcttgagct cgggggtcag cgactcgatc gtccagtcgc cgctcgacgt caccggctcg
     1261 gggacgatgc cctgcttggc ctcgtcctcg gtcggctcgc ggtggtagcg gccctcgagg
     1321 atctggcggc gcagcgcata gatcgtcttg cgctgctggt tcatgacgtc gtcgtactcg
     1381 agcacgttct tgcgctggtc gaagttgcgg ccctcgacct tcttctgcgc gttctcgatc
     1441 gagcgggtca ccatcggcga ctcgatcggg acgtcctcct cgaggccgag ccgctccatc
     1501 aggccggtca tcttgtcgag gccgaagatc cgcagcaggt cgtcctgcag cgacaggtag
     1561 aaccgcgacc ccccggggtc gccctggcgg ccggcgcgtc cacgcagctg gttgtcgatc
     1621 cggcgcgatt cgtggcgctc ggtgccgacg atcttgagcc cgcccgccgc gagcacctcc
     1681 tcgcgctcgg cgtcgcactg cgccttgaag ttcgcgagca gctcctggta ccgcgcctcc
     1741 tcgtcgaccc ggggctcgat cgagccgcgg tacggctcgg gcgggtcgtc gccggccggc
     1801 gcgaccggct gggcgccgtc ggcggtctcg gccagcttgg cgcgctcctc ggcgagcgcc
     1861 tccttggcca tcgcctcggg gttgccgccg agcaggatgt cggtgccgcg gccggccatg
     1921 ttggtcgcga tcgtgatcgc ggccttgcgc ccggcctggg cgacgatcgc cgcctcgcgc
     1981 tggtgctgct tggcgttgag cacgttgaac ggcaggccct tggccttgag cagatttgcg
     2041 accacctcgg acttctcgac cgacaccgtg ccgaccagca cgggctggcc gcgctggtgg
     2101 cagtcctcga cgtcggcgac caccgcgcgg aacttgccgg cctcgttctt gtagaccagg
     2161 tcagggtggt ccttgcggat catcggccgg ttggtcggga tcaccgagac gtcgagcttg
     2221 tagatctggt ggaactcctg ggcctcggtg tcggcggtgc cggtcatccc ggcgagcttc
     2281 ttgtagagcc ggaagtagtt ctggaacgtc accgtggcca gggtctggtt ctcttcctcg
     2341 accgtgacgc cttccttggc ctcgatcgcc tggtgcaggc cgtccgacca ccgccggccc
     2401 tccatcttgc ggccggtgtc ctcgctgatg atgatgacct tgttgttctc gaccaggtaa
     2461 ttgacgttgc gcttgtacag cgtgtgggcg cgcagcgcct ggttgacgtg gtggacgagc
     2521 acgatgttgc tcgccgcgta caggttgtcg acgccgagca gcttctcgat ccgctcgacg
     2581 ccgtcgtcgg tcagcatcgc cgagtgcgcc ttctcgtcga cggtgtagtc gacctcgcgc
     2641 ttgagccggg ggatcagccg atcgatcttc tcgtagagat cggcggacgc ctcgccctgg
     2701 ccggagatga tcagcggggt gcgcgcctcg tcgatcagga tcgagtcgac ctcgtcgacc
     2761 accgcgtagt acaggccgcg ctggaccatg cggtccggcg attccttcat gttgtcgcgc
     2821 aggtagtcga agccgaactc gttgttctgg ccgtaggtga tgtcgcagtt gtagttgttc
     2881 tgccgcgcga tgtcgtcgag gccgtgcacg atcaccccgg tggtcagccc gaggacgctg
     2941 tggatccgcc ccatccactc ggcgtcgcgc ttggcgaggt agtcgttgac cgtgacgacg
     3001 tgcacgccct ggccggtcag cgcgttgagg taggtcggga gcgtcgcgac cagcgtcttg
     3061 ccctcgccgg tccgcatctc ggcgatcttg ccctggtgca gcacgatgcc gccgatcatc
     3121 tggacgtcgt agtgccgcat gccggtccgc cgcacgctgg cctcgcgcac gagcgcgaac
     3181 gcgtcgggga ggatctcgtc gagcgtggcg cccttgtcga gccggcgctt gaactccccg
     3241 gtcatcgccc gcaactcgtc gtcgctcttg gccttgatcg tgggctcgag gtggttgatc
     3301 cgatctacga tcggctggag tttccgcagc tcgcgctggt tccggctacc gaagatcttc
     3361 tggaagattc ccatgggcga agttcgggtc taccacgttc ccgctcgagg attcgtccca
     3421 agggggtcgc gacggccgta acggagatag gtctcgcaaa gctgcagggt tagctgcccg
     3481 atcgagcagg agcgagacgg gggtggggac ccgacggctc cgccggcggg gcgggggcag
     3541 agcccccgtg ggatcgacga gcttcgggga tggccacctc acggccgcgt ggtggcgcga
     3601 agcttcctgc cgaagcagcg tccggatcgg atcgcgacgc gtcgctgcga gcccggccga
     3661 tttcggagat cgccgcgagc gtgacgcgcg tcgccttcga cggtcgttcg ggggcggttt
     3721 gcccgagcga gatcaggtgc ttacggagga gttggaggtc aaatcaggaa gttgccacgc
     3781 gccgcgagct cgctgatcgc gtcgtcggcc gccgccctcg cgctcggcgc tgcgccggcc
     3841 gccgctgcac cgctcgcgcc ggccgccacc gatctcgacc cgatcccgac gtaccaccac
     3901 ttcctgtcga tgcgcgagct cgccgatctg ttcgcgccga gccagggcga catcgcgacc
     3961 atcacgcgct tcccgagcag cttccgcatc accgtgaacg agatgttcgc cgaccggctg
     4021 ctgatcaagg cgaccggcgg gattcccggt tacgaacccg gcgccggcct cgacgtgctc
     4081 gacgtggcaa acttctcgac ggcggtcggt cgcgagctcc gcggtcactg aggatcacgc
     4141 gcgttcggcg gtccgggccc gtgtcggacc gccgagatcg acggttcacc agggagcccg
     4201 accgttcgtc ctgcgaggac gaccgctcgt cactgcagat cttccaccgc gcgatgcggc
     4261 tttacagcgc ggtccgagtc gtccacagtt cgggctcccg tggagcgcga tcagctctcg
     4321 catgacctcg cgtcgttgaa gatcgaccgc gatgcaccac ccgcagggcg ccgctggtcc
     4381 cggctcgtcg ccgcgctcgt cgtcctcgcg gtcctggtcg gcgccgggta cgcgttcggc
     4441 taccgcaagc tgcgagcgtc gctcgcgaca ccggaggtca agatcggcga ggtcgcgctg
     4501 gtgtccccgg tgcagtcgca ggtccagctc acggcgaccg gctacgtcgt cgcgctggtc
     4561 tacgccaagg tcgcctccaa ggtgccgggc cggatcgccg agatcttcgt agaggagggc
     4621 cagacgatcg agaagggatc gcgggtcgcg cggctcgagg acgtcgactt caagacccag
     4681 ctggcgaccg cgcgggcccg cgcggcctcc gcgcgcgcca ggatcgcggt ggcgaaggcg
     4741 agcctggtcg agatcaaggt ccagatcgac cgcgagcggc cgatggtcga caagggggtc
     4801 accgcgaagg cgacgctcga cgacctcgag gcccgccgcg gctcggccga ggccgcggtg
     4861 cgcgccgccg aggccgaggc gatggccacc gacgccgatg tcaaggcgct ggagatccag
     4921 ctcggcagct acgagatcat caccccgatc tcggggaccg tggtcgacaa gctggtcaag
     4981 gtcggcgagg gcgtgtcgcc cggcttcggc acgcccggcg tgatcgaggt catcgatctg
     5041 acgtcgctgg tcgtcgaggt cgacgtgccc gagggccggc tgtcgcaggt cgtggtcgat
     5101 gcgccgtgcg agatcgtgct cgacgcctat ccgacccggc gataccgcgg cgcggtcaag
     5161 gagatcggcc ggcgggtcaa ccgctcgaag gccaccgtgc cggtcaagat ccggttcgtc
     5221 gatctggccg acgggaggcc cgttcccggg gggacttcaa attccgggtc gggcgaggtg
     5281 ggcggccccg gcgcaggcaa gccggagatc ctccccgaca tggcggcgcg ggtcagcttc
     5341 ctgacctcga ccatcgaccc cgcgacgctc gacacgccgc ccaggctgac cgtgccggcg
     5401 ggcgcggtcg tccagcgcaa cggccgcgac gtggtgttcg tcgtcgacga gggcgaggtc
     5461 cggatgacgc cggtcgcgct cggcgcggcc agcggcgacg gtcgcgagct ggtcaccaag
     5521 ctcccccccg gcaccaaggt ggtgatcgat cccccggccg atctcggcga cggccagaag
     5581 gtcaaggaga acaagtgatg agcgagacaa ccagcgagac tgcgaacgaa aca
//
LOCUS       DASVOR010000633         7939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_122804,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000633 DASVOR010000000
VERSION     DASVOR010000633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7939
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122804"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..3471
                     /locus_tag="VF516_12550"
     CDS             406..3471
                     /locus_tag="VF516_12550"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8108552.1"
                     /translation="MSHVQDQVNKSLKLVGRVLGDKFKLTACIGIGGSGAVYRADQIA
                     LGRTVAVKILNEELSADARMIKRFRDEAMSASRLNHPNCVSIIDYGQSPDGLLYLAME
                     YVKGPTLTQLLVNQNPLPIDRVVDIVMQALAGIEEAHLAGVVHADLKADNIILDQRRA
                     GVDIVKIVDFGIARLVTGVREEDRSISGTPEYMAPEVISGSPPSFASDVYAVGIILYE
                     LLAYKTPFFAGSTTEILANHLKAMVPSLAARREHVPKELEGIVARALAKHPGDRFASA
                     ADMRAALASLATRTKKVATRDICAQCGMTCAPSFKFCPECGTPRQRVEQVLPPPPPAA
                     PGEAVRVLPLSFTGRGAELAQLLAHMRRPPSNDVGLLVMGNDGSGRSALLRHAYQHLT
                     GDTRTIYQIGPDPTGLAAPFYPIRSLLAAVLQLAPGASDGELRDAVLASGLDERDVPG
                     ISQLFGHSTTLLELESPVRRREMVWAALRALERAAAAGPVTIVCEDIDRFDHPSLEIL
                     RRATEMIDLQLPPIVMTAHASFGSQWPASVPRLEVAALDAKDLAAIVARLDKAGLKGL
                     PPVQQLFETTRAYPGHLEHVVRYLLEGGKAEDTNISLPDLIAARLSLLKQSTRDVLQA
                     AAVLGLEPPVDLLRSMLPSDALEAALADAERAGLFSHDPSGELTFTSRLVRDIVYDAT
                     PADVRRSLHAAAAAAVEALSPDIALLGHHHDLAGHAKEAIMLLRRAGDHAAEQLDDIG
                     AGQFYYRALVAVRQAVLSGDDDGSAEGQFVMLSVKLADVLRARGETALARGVLAEARD
                     WSGAPMLIALVDRSSAAIALSEGDVEGAIANLRRGVGRAISTGDMNLVCELYLDLATA
                     LLRAGDPAGAQSELVECIDLATLGEGFTAIDGPEPFWRILRAQAQMVDNAGDSYKALR
                     LAEAALFHAQRVRSRLGAARVQHLLAQLCEKTGLGGKAERYRQAAINEMRSLGDRRAT
                     AELLLNDAPTRSALTPTGRRDDAMQLTQDIAWPEGQDGAKRKSSPPSVK"
     gene            3559..3966
                     /locus_tag="VF516_12555"
     CDS             3559..3966
                     /locus_tag="VF516_12555"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8108553.1"
                     /translation="MGSILLVEPDPATSEAWSEAIAVAGHSVATASGIREAWTVVRDG
                     GFDVVVVDAYDPGSGVVDLALGMNALPDAPPIILVSESPDAPWISVRIGAATFVPKPC
                     DPDEVVTAVNRLLWRVRPLPIVDDEPTMRLRQL"
     gene            4015..5367
                     /locus_tag="VF516_12560"
     CDS             4015..5367
                     /locus_tag="VF516_12560"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8108554.1"
                     /translation="MSADDREPRAEPTALRIEIAPRTILLLLVAIAGVWLAFQLETVL
                     VVVTIALVVVGTLDPMVAWFERRGIRRGRALVLIFIGLAALVAGVLLLTVPPLIGQLL
                     HLIEDAPRGRDRLVDWLRQYRWARPLVQTVRAVPVNDLVLRAGNTLVGYSADLLTLVG
                     YAISTTFLAIYLLADPVRARGLLFAVVPRHYHIKLARILIELKVIVGGYMRGQLITSV
                     AIAVFTFGVLEAFRVDDALALALFAGLTDVIPFIGGYVASAPVIIAVTGSGLGAVIIV
                     TALMFAYQEFESRILVPRVYGKVLRLPPAIVVVSLLVGGTLMGIIGALLALPIAAGLQ
                     MVVRELRVELPGETPRDETTRARDERAEQIYEQLSEGATAADAGVIAAELAHQLRDDE
                     QAGATLTVQMPPTTAIRAAVAEAEADHSGTLGALGEPEPAGPRVRRDTAPGIAEDGPE
                     "
     gene            5364..5879
                     /locus_tag="VF516_12565"
     CDS             5364..5879
                     /locus_tag="VF516_12565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108555.1"
                     /translation="MRIWKLVVLLAGVAGVIGFFTPLIDYRSSDGRITGDASAFEIAR
                     GIDRGGDVRAQAEALGLSRADGERISRAVHAGIEAYAGAIVACFVPAGLLTALGLLLM
                     LRDRMGRLAGLCAIALGGASAFVFARFWQADQVSHDTAASLGLGVYLLLAAGLGGVLA
                     GLGALVSPDRG"
     gene            complement(5860..>7939)
                     /locus_tag="VF516_12570"
     CDS             complement(5860..>7939)
                     /locus_tag="VF516_12570"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8108556.1"
                     /translation="EAARPERLSPRVTAYVEEAMRWLPGEAPELAPSRPGDLSGVLIP
                     PDIGDAHIVVADDNADMRDYLRRLLGARWTVTVATDGERALAAAHGNRPDLILTDVMM
                     PNLDGFGLLRALRVDPALSTVPVVMLSARAGEEARIEGIEAGADEYLVKPFSARELLA
                     RVSNMLQLSRLRRDVELERNRLATFIEQTPIAVVLWEGPELRCSLANEAGHRMARRPL
                     TLGTPLRELFPEIAGSESMRCLERVLRDGAPADVVEDRLTLRSPDGSLHEGYYSTSYR
                     PLQDPEGRTTGVIAVAMDVTEQVRARQAVEASRAEAIVASRSKDEFLAMLGHELRNPL
                     APILTALELMRLRAGDVALRERTIIERQTQHLAALVEDLLDVSRIARGMVQLRCERLY
                     VADVIAKAIETAAPLFEQQRHELVTSVPRDLVVDADPGRLTQVFANLLTNAAKYTDPG
                     GRVIVAARRDGGEVVASVTDTGRGIAADMLGRVFELFVQERQNLDRARGGLGLGLAIV
                     RNLVALHGGRVEVSSPGPRLGSTFTVRLPFAPDSATGAEAAQPASGPDAGPAHAISVL
                     VVDDNVDAATLLGDLLSARGYRTCIAHDAAEVLQLARDFVPDVALLDIGLPAMDGYEL
                     ARRLRELPAWRHVRLLALTGYGQSTDRARSVDVGFDHHLVKPIDLATLERYLPPAGPG
                     ARPGLSRDPG"
BASE COUNT         1056 a         2892 c         2880 g         1111 t
ORIGIN      
        1 gctcgcggcg agctcgcgct cgagcaggcg caggacggcc gggtgacgga tcgcgagcca
       61 ggcggcggcg gcggtggcgc ggccgggggc gcggtcagcg gcggagtcga gcgagaggcg
      121 gtccggtgct cgatggtgga acatgctcga ccctaggcac cgcccgatcc ggcgcgccag
      181 ttttcggagg atcgtcggcg atggtatagt cgagccgata agggggcgct cacgatcggc
      241 gcggcctcca ggcaaggcac tgaaatgaca cagcgaaatg ccgagggcgc gggacgcatg
      301 ttccgcggca ggaaacgttc ggtgcggcgc gcggacggga taatgtggag ggtggagtga
      361 tcgcgtgacg tcggcgcgcc cagcagtccg tcgcaggtgg ccgacgtgag ccacgtccag
      421 gaccaggtca acaagtcgct gaagctggtc ggacgcgtcc tcggcgacaa gttcaagctc
      481 accgcgtgca tcgggatcgg cggcagcggc gcggtgtatc gcgccgacca gatcgcgctc
      541 ggccggaccg tcgcggtcaa gatcctgaac gaggagctgt cggccgacgc gcggatgatc
      601 aagcggttcc gcgacgaggc gatgagcgcg tcgcggctca accaccccaa ctgcgtctcg
      661 atcatcgact acggccagtc gcccgacggg ctgctctacc tcgcgatgga gtacgtcaag
      721 ggcccgacct tgacccagct gctggtcaac cagaacccgc tgccgatcga tcgcgtggtc
      781 gacatcgtga tgcaggcgct cgccgggatc gaggaagccc acctcgcggg cgtggtccac
      841 gccgacctca aggccgacaa catcatcctc gaccagcgcc gggccggggt cgacatcgtc
      901 aagatcgtcg acttcgggat cgcgcggctg gtcaccggcg tgcgcgagga ggatcgctcg
      961 atcagcggca cgccggagta catggcgccc gaggtgatca gcggctcgcc gccgagcttc
     1021 gcctcggacg tctacgcggt cggcatcatc ctgtacgagc tcctggccta caagacgccg
     1081 ttcttcgcgg gctcgaccac cgagatcctc gccaaccacc tcaaggcgat ggtgccgtcg
     1141 ctggcggcgc gccgcgagca cgtgcccaag gagctcgagg gcatcgtcgc ccgcgcgctc
     1201 gccaagcacc ccggcgaccg gttcgcgtcg gcggccgaca tgcgggccgc gctggccagc
     1261 ctggcgaccc ggaccaagaa ggtcgcgacc cgcgacatct gcgcccagtg cggcatgacc
     1321 tgtgcaccga gcttcaagtt ctgtcccgaa tgtggaacgc cgcggcagcg cgtcgagcag
     1381 gtcctgccgc cgcccccgcc cgccgcgccg ggcgaggccg tccgggtcct gccgctttcg
     1441 ttcaccggcc gcggcgcgga gctcgcccag ctgctcgccc acatgcggcg cccgccgagc
     1501 aacgacgtcg gcctgctggt catgggcaac gacggctcgg gccgcagcgc gctgctccgc
     1561 cacgcctacc agcacctcac cggcgacacc cggacgatct accagatcgg ccccgacccg
     1621 accgggctgg cggcgccgtt ctacccgatc cgctcgctgc tcgccgcggt gctgcagctg
     1681 gcgccgggcg cgagcgacgg cgagctgcgc gacgcggtgc tcgccagcgg cctcgacgag
     1741 cgcgacgtgc ccgggatctc gcagctgttc ggccacagca cgacgctgct cgagctcgag
     1801 tcgccggtgc ggcgccgcga gatggtgtgg gcagcgctgc gcgccctgga gcgcgcggcc
     1861 gcggcggggc cggtgacgat cgtgtgcgag gacatcgacc gcttcgatca cccgagcctc
     1921 gagatcctgc ggcgcgcgac cgagatgatc gatctccagc tgccgccgat cgtgatgacc
     1981 gcgcacgcgt cgttcggctc gcagtggccg gcctcggtgc cgcgcctcga ggtcgccgcg
     2041 ctcgacgcca aggacctcgc cgcgatcgtc gcgcggctcg acaaggccgg gctcaagggc
     2101 ctgccgccgg tccagcagct gttcgagacc acgcgggcct accccgggca cctcgagcac
     2161 gtcgtgcgct acctgctcga gggcggcaag gccgaggaca ccaacatctc gctgcccgac
     2221 ctgatcgccg cccggctgtc gctgctcaag cagtcgaccc gcgacgtgct gcaggccgcc
     2281 gccgtgctcg gcctcgagcc cccggtcgac ctgctgcgct cgatgctgcc gagcgacgcg
     2341 ctcgaggccg cgctggccga cgccgagcgc gccgggctgt tcagccacga cccgtcgggc
     2401 gagctcacgt tcaccagccg gctggtccgc gacatcgtgt acgacgccac gccggccgac
     2461 gtccggcgct cgctgcacgc ggccgccgcc gccgcggtcg aggcgctgtc gccggacatc
     2521 gcgctgctcg gccaccacca cgacctcgcc ggccacgcca aggaggcgat catgctgctg
     2581 cgccgcgccg gcgaccacgc cgcggagcag ctcgacgaca tcggcgccgg ccagttctac
     2641 taccgcgcgc tggtcgcggt gcgccaggcc gtgctgagcg gcgacgacga cggcagcgcc
     2701 gagggccagt tcgtcatgct gtcggtcaag ctcgccgacg tgctgcgcgc ccgcggcgag
     2761 accgcgctgg cccgcggcgt gctggccgag gcgcgcgact ggtcgggcgc gccgatgctg
     2821 atcgcgctgg tcgaccgctc gagcgccgcg atcgcgctca gcgagggcga cgtcgagggc
     2881 gcgatcgcca acctgcggcg cggcgtcggc cgggcgatct cgaccggcga catgaacctg
     2941 gtgtgcgagc tctatctcga cctcgcgacc gcgctgctgc gcgccggcga tccggccggg
     3001 gcgcagagcg agctggtcga gtgcatcgat ctcgccacgc tcggcgaggg cttcaccgcg
     3061 atcgacggcc cggagccgtt ctggcgcatc ctgcgcgcac aggcccagat ggtcgacaac
     3121 gccggcgact cctacaaggc gctgcggctc gccgaggctg cgctgttcca cgcccagcgc
     3181 gtgcggtcgc ggctcggcgc cgcccgggtc cagcacctgc tcgcccagct gtgcgagaag
     3241 accgggctgg gcggtaaggc cgagcgctac cgccaggccg cgatcaacga gatgcgcagc
     3301 ctgggcgatc gccgcgcgac cgccgagctg ctgctcaatg acgcgccgac ccggtccgcc
     3361 ctcacgccga ccggccggcg cgatgacgcg atgcagctga cccaggacat tgcctggccg
     3421 gagggtcaag acggcgcgaa gcgcaagtcg agtccaccct cggtcaagta gtcagaaaag
     3481 ttcgactatg ctggaagatg tggtctggac cgcatttact cttgcttgtt tttgggttgt
     3541 ccagcatacc tatttctcat ggggagtatc ttgctcgtcg agcccgatcc agccaccagc
     3601 gaggcgtggt ccgaggcgat cgccgtcgcg ggacattcgg tggcgaccgc gagcggaatc
     3661 cgggaggcgt ggaccgtcgt ccgcgacggc ggcttcgacg tcgtcgtggt cgacgcgtac
     3721 gacccgggct ccggcgtcgt ggatctcgcc ctgggcatga acgcgctacc ggacgcgccg
     3781 ccgatcatcc tggtctcgga gtccccggac gcgccgtgga tctcggtccg gatcggggcg
     3841 gcgacgttcg tgcccaagcc gtgcgacccg gacgaggtgg tcacggcggt caaccgcttg
     3901 ctgtggcgtg tgcgcccact gccgatcgtc gatgacgagc cgacgatgcg gttgcgacag
     3961 ctgtaggacg gtccggactg cggcacggcg cttgcggtgc gatagtgcaa gaccgtgagc
     4021 gccgacgacc gagagccccg ggcggaaccg accgcgctgc ggatcgagat tgccccgcgg
     4081 acgatcctgc tgctgctcgt cgcgatcgcc ggggtctggc tggcgttcca gctcgagacc
     4141 gtgctcgtcg tcgtgacgat cgcgctggtc gtggtcggca cgctcgatcc gatggtggcg
     4201 tggttcgagc gccgcgggat ccggcgcggc cgcgcgctgg tcctgatctt catcgggctc
     4261 gccgccctgg tcgcgggcgt gctgctgctc accgtgccgc cgctgatcgg ccagctgctg
     4321 cacctgatcg aggatgcgcc gcgcggccgc gatcgcctgg tcgactggct gcgccagtac
     4381 cgctgggcgc ggccgctggt ccagaccgtc cgcgccgtgc cggtcaacga cctcgtgctg
     4441 cgcgccggca acacgctggt cggctactcg gccgacctcc tgaccctggt cggctacgcg
     4501 atctcgacca cgttcctcgc gatctacctg ctcgccgacc cggtgcgcgc gcggggcctt
     4561 ctgttcgcgg tggtcccgcg ccactaccac atcaagctcg cgcgcatcct gatcgagctc
     4621 aaggtcatcg tcggcggcta catgcgcggc cagctgatca cctcggtcgc gatcgcggtg
     4681 ttcacgttcg gcgtgctcga ggcgttccgg gtcgacgacg cgctcgcgct cgcgctgttc
     4741 gccgggctga ccgacgtcat cccgttcatc ggcggctacg tcgccagcgc gccggtgatc
     4801 atcgcggtca ccggctcggg gctcggcgcg gtgatcatcg tgaccgcgct gatgttcgcc
     4861 taccaggagt tcgagagccg gatcctggtg ccgcgggtct acggcaaggt gctgcgcctg
     4921 ccgcccgcga tcgtcgtcgt gtcgctgctg gtcggcggaa cgctgatggg catcatcggc
     4981 gcgctgctcg cgctgccgat cgccgccggg ctgcagatgg tggtgcgcga gctccgcgtc
     5041 gagctgcccg gcgagacccc gcgggacgag accacgcgcg cgcgcgacga gcgcgccgag
     5101 cagatctacg agcagctgtc ggagggcgcg accgccgccg acgccggcgt gatcgcggcc
     5161 gagctcgccc accagctgcg cgacgacgag caggccgggg cgacgctcac cgtccagatg
     5221 ccgccgacga cggcgatccg ggccgcggtc gccgaggccg aggccgacca cagcgggacg
     5281 ctgggcgcgc tcggcgagcc cgagcccgcc gggccgcggg tccggcgtga cacggcgccg
     5341 gggatcgccg aggatgggcc ggaatgagga tctggaagct cgtcgtcctg ctcgccggcg
     5401 tcgccggcgt gatcggcttc ttcaccccgc tgatcgacta ccgcagcagc gacggcagga
     5461 tcaccggcga cgccagcgcg ttcgagatcg cgcgcgggat cgaccgcggc ggggacgtcc
     5521 gcgcccaggc cgaggcgctc gggctgtcgc gcgccgacgg tgagcggatc tcccgggcgg
     5581 tccacgccgg catcgaggcc tacgccggcg cgatcgtcgc ctgcttcgtg ccggccgggc
     5641 tgctcaccgc gctcggtctg ctgctgatgc tgcgcgatcg catgggccgg ctcgccgggc
     5701 tgtgtgcgat cgcgctcggc ggcgcgtcgg cctttgtgtt cgcccggttc tggcaggccg
     5761 atcaggtctc gcacgacacg gccgcgtcgc tcggcctcgg cgtgtacctc ctgctcgcgg
     5821 ccggcctggg cggggtgctc gcggggctcg gcgcgctggt cagcccggat cgcggctgag
     5881 ccccggacgc gcgcccggac ccgccggcgg caggtagcgc tccagcgtgg cgagatcgat
     5941 cggcttgacc aggtggtgat cgaagccgac gtcgaccgag cgcgcgcggt cggtgctctg
     6001 gccgtagccg gtcagcgcga gcagccggac atggcgccac gccggcagct cgcgcagccg
     6061 gcgcgcgagc tcgtagccgt ccatcgccgg caggccgatg tcgagcagcg cgacgtcggg
     6121 cacgaagtca cgggcgagct gcagcacctc cgcggcgtcg tgcgcgatgc aggtccggta
     6181 acccctcgcg ctcagcagat cgccgagcag ggtcgcggca tcgacgttgt cgtcgaccac
     6241 cagcaccgag atcgcatgcg ccggcccggc gtccggcccc gatgcgggct gcgccgcctc
     6301 cgcgcccgtc gcgctgtccg gcgcgaacgg caggcggacc gtgaacgtgc tgcccaggcg
     6361 ggggcccggg ctcgacacct cgacccgccc gccgtgcagc gcgacgaggt tgcgcacgat
     6421 cgcgagcccg aggccgaggc cgccgcgcgc gcgatcgagg ttctggcgct cctggacgaa
     6481 cagttcgaac acccggccga gcatgtcggc cgcgatgccg cgcccggtgt cggtcaccga
     6541 ggcgacgacc tcgccgccgt cgcgcctcgc cgcgacgatc acccggccgc ccgggtcggt
     6601 gtacttggcc gcgttggtca acaggtttgc gaacacctgg gtcagccgcc ccggatcggc
     6661 gtcgaccacg aggtcgcgcg gcaccgaggt caccagctcg tggcgctgct gctcgaacag
     6721 cggcgccgcg gtctcgatcg ccttggcgat gacgtcggcg acgtacagcc gctcgcaccg
     6781 cagctgcacc atgccgcggg cgatccgcga cacgtcgagc aggtcctcga ccagcgcggc
     6841 caggtgctgg gtctggcgct cgatgatcgt ccgctcgcgg agcgccacgt cgccggcgcg
     6901 cagccgcatc agctccagcg cggtcaggat cggcgcgagc gggttgcgca gctcgtggcc
     6961 gagcatcgcg aggaactcgt ccttggaccg gctggcgacg atcgcctcgg cgcggctggc
     7021 ctcgaccgcc tggcgggccc ggacctgctc ggtcacgtcc atcgccaccg cgatcacgcc
     7081 ggtggtccgg ccctcggggt cctggagcgg ccggtacgag gtgctgtagt agccctcgtg
     7141 cagcgagccg tcgggggacc gcagggtcag ccggtcctcg accacgtcgg ccggcgcgcc
     7201 gtcgcgcagc acgcgctcga ggcagcgcat cgactcgctg cccgcgatct cggggaacag
     7261 ctcgcgcagc ggtgtgccca gggtcagcgg ccgccgcgcc atccggtggc cggcctcgtt
     7321 ggccaggctg caccgcagct ccgggccctc ccacagcacg acggcgatcg gggtctgctc
     7381 gatgaacgtg gcgagccggt tgcgctcgag ctcgacgtcg cgccgcagcc gtgacagctg
     7441 gagcatgttc gacacccgcg cgagcagctc gcgggccgag aacggcttga ccaggtactc
     7501 gtcggccccg gcctcgatgc cctcgatccg cgcctcctcg ccggcgcgcg ccgacagcat
     7561 cacgaccggc acggtcgaca gcgccgggtc gacgcgcagc gcgcggagca ggccgaagcc
     7621 gtcgaggttc ggcatcatca catcggtcag gatcaggtcc gggcggttgc cgtgcgccgc
     7681 cgccagggcg cgctcgccgt cggtggccac cgtcaccgtc cagcgcgccc cgagcagccg
     7741 gcgcaggtag tcgcgcatgt cggcgttgtc gtcggcgacg acgatgtgcg cgtcgccgat
     7801 gtccggcggg atcagcacgc cggacagatc gccggggcgg ctcggcgcga gctccggcgc
     7861 ctcgccgggc agccagcgca tcgcctcctc gacgtacgcg gtcacgcgcg gcgacagccg
     7921 ctccggacgg gcggcctcg
//
LOCUS       DASVOR010000634         6290 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_502837,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000634 DASVOR010000000
VERSION     DASVOR010000634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6290
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502837"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF516_12575"
     CDS             complement(<1..347)
                     /locus_tag="VF516_12575"
                     /inference="COORDINATES: protein motif:HMM:NF015377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX8108557.1"
                     /translation="MSGAAPTWYTISMWRCRLPAALIVLSACAGSHTRELAVPDVRQA
                     TDYTCSASALQAVLAYYGVEMREDELARELGATPEDGAPPEAIVRVAVAHGLSATKRE
                     HMTVDDLAAEIARG"
     gene            425..2488
                     /locus_tag="VF516_12580"
     CDS             425..2488
                     /locus_tag="VF516_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coagulation factor 5/8 type domain-containing
                     protein"
                     /protein_id="HEX8108558.1"
                     /translation="MKRALHPARLAVAVSRVLLVVIACSAPATAATDFTQTVTQLSAS
                     QVQVSFTPTTPATFVDLHYIPPPTPPIGQQNVRMTNNAGTWQFTIGGLTSGFVLEYWF
                     TYDKNGAAFDSTHFTFTVGGGSTGTCPTQSDIPNFGPNVRIFDPGMATTTIQAQLDAD
                     FNAQKDTQTAQMGTGRIAHLFKPGSYTVHDNVGFYTSVAGLGQNPDDVTINGDVTVDA
                     FNASDQGVALQNFWRSAENLAINPSSGSDRWAVAQAAPFRRIDVHGNLLLAPASNGFA
                     SGGYIADTRVSGQTSSVSQQQWYTRDSSLGSWSGGVWNMVFSGVGGAPSNTFPSPPET
                     TLATTPVSRDVPYLFIDSTGKYRVFLPSLRTSASGPSWASGSTPGTSLPMSQFYVVKA
                     SDTASTINSALASGCNLFFPPGIYHVNQTLNVTRAGTILLGIGYPTVVPDSGVNAMQI
                     ADVDGIRIKGLLFDAGTTSSNALLTVGPSGATASHASNPTTIQDVFFRIGGATVGKAT
                     NSLVVNSNDVLVDHTWAWRADHGNAGTVGWTINTADTGVLVNGNNVLATGLFVEHYQK
                     FQVVWNGQHGKTIFFQNEMPYDVPDQASWNSASGVNGFAAYKVGNNVTSHEGWGLGAY
                     CFFNTNPAVSASHAFEVPTATGVLFHDILTVSLNFKGVITHVINNTGATTPSGTVPVN
                     VVSFP"
     gene            complement(3358..5049)
                     /locus_tag="VF516_12585"
     CDS             complement(3358..5049)
                     /locus_tag="VF516_12585"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8108559.1"
                     /translation="MDQVKGAAEALLRLLRAQEEIAYSLLLERSFPAEAATFAMSVAL
                     LRKGRSLDEAAGASRAAYQSLDDSSRKQLDELRDTRAKLVELTMAGSSDTAELQHLAD
                     HADEVEQALAKQSAPLRARRLLARPSEIVTRVAQALPPDGVFVEVVAFRPSAVRAPRQ
                     DAGSGPQCYLALILHRDASVRVVPLGPAAPIDQLVSDFRAQLTVDPKDGDDTHQVLTA
                     SEALYHRVFEPLRAQFGKADKNIFLSLDSQLHLVPFAALFEGNQYLVETYQFTYLTSG
                     RDLLRGEVGPRNSTTVAMIANPEFIKGRARGPALRSECDRRGLSMVPDRSQSGTHDAA
                     PYRSFRLTSLCPLPGTVEETQDLRQLLPAVRVLLGTNATRQAFLGLRSPGILHVATHG
                     LFRRGTDDAAPGSRTEIESDRAALLNSMLMLAGAGVKSNGEVVVQPDGVVTALDVAGM
                     NLWGTQLVVLSACETGVGDVSNLGQGVYGLRRAVMIAGAETLVTSLWKVDDRATRELM
                     RRYYESLLAGHGRVEAMRETADWMRRRRKHPYYWASFIVVGSRNPLVGIGIDRKQ"
     gene            5428..5589
                     /locus_tag="VF516_12590"
     CDS             5428..5589
                     /locus_tag="VF516_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108560.1"
                     /translation="MELQDKVRRIVDEAARLSRAGFDRGVREIVREFGQKWADAGRCH
                     APASPQKPP"
     gene            5699..>6290
                     /locus_tag="VF516_12595"
     CDS             5699..>6290
                     /locus_tag="VF516_12595"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX8108561.1"
                     /translation="MASIAELGERLAALRLCEAHALDAMRRSLDRHGQLTPIVAFAER
                     DQIQTLDGFKRLRAARALGWPTVAITIAEVADGIDAKIRLAELHQHRGLTELEQGWLV
                     RSLYRDDKLSQPEIARRLDRDKSWVCRRLILVEALDLAVQADVRLGLIAPRAAIALAA
                     LPRGNQVAASAVVIRRGLTVAQTERLVAELREQPEAA"
BASE COUNT         1066 a         2151 c         2091 g          982 t
ORIGIN      
        1 ccccgggcga tctcggccgc cagatcgtcg acggtcatgt gctcgcgctt ggtggcgctc
       61 aggccgtgcg cgaccgcgac gcggacgatc gcctcgggcg gcgctccgtc ctccggcgtg
      121 gcgcccagct cgcgtgcaag ctcgtcctcg cgcatctcga ccccgtagta cgcgagcacc
      181 gcctgcagcg ccgacgcgct acaggtgtag tccgtggcct ggcgaacatc cggcaccgcg
      241 agctcgcgcg tgtgcgagcc ggcgcaggcc gacagcacga tcaacgccgc tggcaggcga
      301 catcgccaca tcgagatcgt gtaccacgtc ggggctgctc cggacatgac gaccgcctcc
      361 tgccctccgt gcgcgacagc gcaatcccat tgatttgatt tcatgcttgc gcttacgtcg
      421 tcgcatgaag cgagctctcc atcctgccag gcttgccgtc gccgtgtcgc gggtcttgct
      481 ggtcgtcatc gcctgcagcg cgcccgcgac ggccgcgacc gacttcaccc agaccgtcac
      541 ccagctgagc gcatcgcagg tccaggtctc gttcacgccg accacgccgg cgacgttcgt
      601 cgacctccac tacatcccac cgcccacgcc gcccatcggt cagcagaacg tccggatgac
      661 caacaacgcc gggacgtggc agttcaccat cggcgggctg accagcggct tcgtgctgga
      721 gtactggttc acctacgaca agaacggcgc ggccttcgac tcgacgcact tcacgttcac
      781 cgtgggcggc ggtagcaccg ggacgtgccc gacccagtcc gacatcccga acttcggccc
      841 caacgtccgg atcttcgatc ccggcatggc caccacgacc atccaggcgc agctcgacgc
      901 cgacttcaac gcccagaagg acacccagac cgcccagatg ggcaccgggc ggatcgcgca
      961 cctgttcaag ccgggctcct acaccgtcca cgacaacgtc ggcttctaca cctcggtcgc
     1021 aggtctgggg cagaaccccg acgacgtgac catcaacggg gacgtgaccg tcgacgcctt
     1081 caacgcctcg gaccagggcg tggcgctgca gaacttctgg cggtccgccg agaacctggc
     1141 gatcaacccg agcagcgggt ccgatcgctg ggccgtggcg caggccgcgc cgttccgccg
     1201 catcgacgtg cacggcaacc tgctgctggc cccggcgagc aacggcttcg cgagcggcgg
     1261 ctacatcgcc gacaccaggg tctccggcca gacctcgtcg gtctcgcagc agcagtggta
     1321 cacgcgagac tcgagcctcg gcagctggtc cggcggcgtg tggaacatgg tgttctcggg
     1381 cgtcggcggc gcgccctcga acacgttccc gagcccgccg gagaccacgc tcgccaccac
     1441 cccggtctcg cgcgacgtgc cgtacctgtt catcgacagc acgggcaagt accgcgtgtt
     1501 cctgccgtcg ctgcgcacca gcgcctccgg cccgagctgg gcgagcggca gcacgcccgg
     1561 cacgtcgctg ccgatgagcc agttctacgt cgtcaaggcc agcgacaccg cgtcgaccat
     1621 caactccgcg ctggcctccg gctgcaacct gttcttcccc ccggggatct accacgtcaa
     1681 ccagaccctc aacgtgaccc gggccggcac catcctcctc ggcatcggct acccgaccgt
     1741 ggtcccggac agcggcgtca acgccatgca gatcgccgac gtcgacggca tccggatcaa
     1801 gggcctcctg ttcgacgccg gcacgacgag ctcgaacgcg ctcttgaccg tcggcccgtc
     1861 cggtgcaacc gcgagccacg ccagcaaccc gacgacgatc caggacgtgt tcttccggat
     1921 cggcggcgcg accgtcggca aggcgaccaa cagcctcgtc gtcaacagca atgacgtcct
     1981 cgtcgatcac acctgggcct ggcgcgccga tcacggcaac gccggcacgg taggctggac
     2041 gatcaacacg gcggacaccg gggtgctggt caacggcaac aacgtcctcg cgaccggcct
     2101 gttcgtcgag cattaccaga agttccaggt ggtctggaat ggccagcatg gaaagaccat
     2161 cttcttccag aacgagatgc cgtacgacgt gcccgatcag gcatcgtgga acagcgcctc
     2221 cggcgtcaac ggcttcgccg catacaaggt gggcaacaac gtcaccagcc acgagggctg
     2281 gggtctgggc gcctattgct tcttcaacac caaccccgcg gtgagcgcct cccacgcctt
     2341 cgaggtgccg accgccaccg gcgtgctgtt ccacgacatc ttgaccgtgt cgctcaactt
     2401 caagggcgtc atcacccacg tgatcaacaa caccggcgcg accacgccct cgggcaccgt
     2461 cccggtcaac gtggtgagct tcccctgagt cgaccgccgc gcttcggagc ggggccctga
     2521 gctcgcaact cgccttccgt caccgcgcgg acgtggtcgg gccggtcgtc aaccgggcgg
     2581 aaaattcttc gttgagagga tcagcgtaag gtcaccggcg cctggccgac gcgtggaggt
     2641 ggtgtatagg cgcagtatcg gaaggcaata ttctcaaatt gaagagtcgg acgctgaggc
     2701 tggatacgtc tttgttagct taaccaaagt agccgattat gaagcattct atcgattgaa
     2761 ctcagatgac acacgtcgag tgttacatcc gttcaatctt cgacagatat ccgaggcgga
     2821 agagaagcga ctggaaggtg ataatatagg acttgcagac cccaggctga ctcatgaact
     2881 ggtagccgaa taccgcggcc gaaaggcatg gcctggtgtg atcaacttcg tgggcactgt
     2941 cgctctagca ggagttcccg aagcattcgc cgggatgact gtgcagcatg tcttcaaaat
     3001 tggcaggtct cacgagtccc tttacctttt tcaagacggc cttaacagta cgtcgacgga
     3061 ctatcgaccg gcgaacctta taggtctgca agccattcgc cgcaagccat aggcgtcgcc
     3121 agcgcaccgg cggccccggc cgagccggtc ggcacgaccc tgggccgcac gcggatagca
     3181 ggaagtgttg ccgtcgtggc gtcgagcgat cgtcctcgtc cagcccgaga tcattctgcc
     3241 ttggcatcgc gccagctttc cgcttctggc agcgccgatc ccggccgcga aagacgtcgc
     3301 cattgccacc cgggaccatc aacctgatcc gcgacatggc ggcgcggtgg atccgcgcta
     3361 ttgcttcctg tcgataccga taccgacgag cggattgcgc gagccgacaa cgatgaacga
     3421 tgcccagtag tacgggtgct tgcgccgccg gcgcatccaa tcggccgtct cgcgcattgc
     3481 ttcgacgcgg ccgtggccag cgagcaagct ctcgtagtag cggcgcatca gctcgcgggt
     3541 cgctcggtca tcgaccttcc agaggctagt gaccaaggtc tctgcgccgg cgatcatcac
     3601 ggcgcggcgc aggccgtaaa cgccctggcc gaggttgctg acatctccga cgccggtctc
     3661 gcaggcggag agcacgacca gctgggtgcc ccacaggttc atcccagcaa catcgagcgc
     3721 cgtgaccact ccgtccggct gaacgacgac ctcgccgttg ctcttaacgc cagcgccggc
     3781 cagcatcagc atggagttca acagcgcggc ccggtcgctc tcgatctcgg tgcgcgagcc
     3841 cggcgccgca tcgtcggtgc cccgacggaa caggccatgc gtggcgacgt ggaggatgcc
     3901 gggcgaccga aggccgagga acgcctgccg ggtggcgttg gttcccagaa gcacccggac
     3961 ggcaggtagc agctggcgga gatcctgcgt ttcctcgacc gtgcccggaa gcggacagag
     4021 ggacgtgagc cggaacgaac ggtatggcgc tgcgtcgtgc gtccccgatt gcgagcggtc
     4081 cggcaccatg ctcaggccac ggcgatcgca ctccgaccta agggccggcc ccctcgccct
     4141 tcccttgatg aactccgggt tggcgatcat cgccactgtc gtgctgttcc gcgggccgac
     4201 ctcgccgcgc agcagatcgc ggcccgaggt gagataggtg aactggtagg tctcgacgag
     4261 atactggttc ccctcaaaca gggcggcgaa cggcaccagg tggagctggc tgtcgagcga
     4321 caggaagatg ttcttgtccg ccttgccgaa ctgggcgcgc agcggctcga acacgcggtg
     4381 gtacagcgcc tcggaagctg tgagcacctg gtgagtgtca tcgccgtcct tcgggtcaac
     4441 ggtgagttgc gcacggaagt cgctgacgag ctggtcgatc ggggccgccg gaccgagggg
     4501 tacgacgcgt acgctcgcgt cgcgatggag gatcagggcc aagtagcact gtgggccact
     4561 gcctgcgtct tggcgcgggg cgcgaacggc ggacgggcga aacgcgacga cctccacgaa
     4621 cacgccatcg gggggcaggg cctgcgcgac gcgcgtgacg atctcgctgg gtcgggcgag
     4681 caggcgcctg gcgcgcagcg gtgcggattg cttcgccagt gcctgctcga cctcgtcggc
     4741 gtgatccgcg aggtgctgga gttcggcggt atcgctgctg ccggccatgg tgagctcgac
     4801 gagcttggcg cgggtgtcgc gaagctcgtc gagttgcttg cgcgaactgt catctaagct
     4861 ctggtaggcc gcgcgcgacg cccccgcggc ctcgtcgagg gaccggccct tgcgcaggag
     4921 tgctaccgac atcgcgaacg tggcggcctc ggcggggaac gacctttcaa gcaggagtga
     4981 gtaggcgatc tcctcctgcg cacgcaggag acggagcaat gcttcagcgg cgcctttgac
     5041 ctgatccact tcgcggcgct ttcgtgatcc agtccggcgc ataggcggcg gccggcgcgg
     5101 cgctttcgtg atccacctcg gcggcggagc cgcaggctga gagctccgcg ctgcgcgggg
     5161 gcgtgacgct gcgccatggc gaagcgtccg cgatgcacgg aatgccggag gcggtacacg
     5221 ccggcggcga cgacgagcga tcggcagaag acgtgcgggc cggcatgccg gctggtgcga
     5281 cgccggaagc aagcgcgggc gcgacgccgc cgcgatcccg agggctatcg cgccgcggag
     5341 cgagcgcggc aggacgtgca ccgggaagga ccgcaggagg agacggacgg gtccggatgt
     5401 cacgcgccgg cttcgccggc tatcccggtg gagttgcagg acaaagtgcg tcgaatcgtg
     5461 gacgaggcgg cgcgcctgtc acgcgccggc ttcgaccgcg gtgtgcgcga aatcgtgagg
     5521 gaattcggcc aaaaatgggc ggacgcgggc cgctgtcacg cgccggcttc gccgcagaag
     5581 ccaccgtgat cacgagggaa tcgcgccgaa aatgggcgcg tgtcacacgc cggcttggtg
     5641 tggcggcgcc ctccggtgct cggatgcagc cgtgactgca aaccagccac cgccacggat
     5701 ggcgagcatc gcggagctgg gcgagcgact cgccgcgctc cggctgtgcg aggcacacgc
     5761 gctcgacgcg atgcgacgct cgctcgatcg ccacgggcag ctcacaccga tcgtcgcgtt
     5821 cgccgagcgc gatcagatcc agacgttgga cggcttcaag cgcctgcgtg cggcgcgcgc
     5881 gctgggctgg ccgacggtgg cgatcacgat cgccgaggtc gccgacggga tcgacgccaa
     5941 gatccggctg gcggagctgc accagcaccg cgggctgacc gagctcgagc agggctggct
     6001 ggtccggtcg ctgtaccgcg acgacaagct ctcgcagccc gagatcgcgc gccggctgga
     6061 tcgcgacaag agctgggtgt gccggcggct gatcctggtc gaggcgctcg acctcgcggt
     6121 ccaggccgac gtgcggctgg ggctgatcgc gccgcgcgcg gcgatcgctc tggccgcgtt
     6181 gccgcgtggc aaccaggtgg cggcctcggc ggtggtgatc cgccgcggat tgaccgtcgc
     6241 gcagaccgag cggctggtcg ccgagctgcg ggagcagccc gaggccgccc
//
LOCUS       DASVOR010000635         2208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_239711,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000635 DASVOR010000000
VERSION     DASVOR010000635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239711"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1392
                     /gene="fumC"
                     /locus_tag="VF516_12600"
     CDS             <1..1392
                     /gene="fumC"
                     /locus_tag="VF516_12600"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEX8108562.1"
                     /translation="TRTETDSMGPIEVDAARYWGAQTERSLHHFRIGSERMPRELIHA
                     LALIKKASAQVNRELGLLSDAKAQAIAAAADEILRGDHDGEFPLVVWQTGSGTQTNMN
                     VNEVTANRASELAGAGRGRDRSIHPNDDVNRSQSSNDVFPTAMHIAAAERTTRHLLPA
                     LAALQGTLASKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAQLDHAAAAIRAALPA
                     VHELALGGTAVGTGLNTHPAYAVRVAARLAELTGLPLVTAPNKFAALAGHDALVGLHG
                     ALKQLATALTKIANDVRWLASGPRSGLGELRIPENEPGSSIMPGKVNPTQCEAMTMVC
                     AQVVGNDVAIAIGAASGNFELNVYKPLIAHNALQSIRLLGDACASFDVHCARGLEPDR
                     AEIERKLHGSLMLVTALAPHIGYDKAAQVAKHAHAHGTLLRDAAIALGYVTGDDFDRW
                     VRPEAMVGPAAER"
     gene            complement(1405..1749)
                     /locus_tag="VF516_12605"
     CDS             complement(1405..1749)
                     /locus_tag="VF516_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108563.1"
                     /translation="MTMPTRQELLVKFGPVDDPEIEARRQHILEALLESSPETRQRLI
                     DKGRAEGLVKGEEKGQLLEARRALRRVLAGRQLTPSQTDEARINACPDLATLERWLDQ
                     AITARSVTEALR"
     gene            complement(2016..>2208)
                     /locus_tag="VF516_12610"
     CDS             complement(2016..>2208)
                     /locus_tag="VF516_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108564.1"
                     /translation="IGSIDTTIKDVQLGPVKMTADRLHFDGIDELEVEFDGFRPVRAT
                     VVVHRVTATHSTLQISSRS"
BASE COUNT          338 a          838 c          710 g          322 t
ORIGIN      
        1 acccgcaccg agaccgacag catgggcccg atcgaggtcg acgccgcccg ttactggggc
       61 gcccagaccg agcgctcgct ccaccacttc cggatcggca gcgagcgcat gccgcgcgag
      121 ctgatccacg cgctcgcgct gatcaagaag gcgtcggcgc aggtcaaccg cgagctcggc
      181 ctgctgtccg acgccaaggc ccaggcgatc gccgccgcgg ccgacgagat cctgcgcggc
      241 gatcacgacg gcgagttccc gctcgtcgtg tggcagaccg gcagcggcac ccagaccaac
      301 atgaacgtca acgaggtcac cgccaaccgc gcgagcgagc tcgccggcgc cggccgcggc
      361 cgcgatcggt cgatccaccc gaacgacgac gtcaaccgct cgcagagctc caacgacgtg
      421 ttccccacgg cgatgcacat cgccgccgcc gagcgcacca cccgccacct cttgcccgcg
      481 ctcgccgcgc tgcagggcac cctggccagc aagtcgcgcg cgttcgccga catcgtcaag
      541 atcggccgca cccacctgca ggacgccacg ccgctcaccc tcggtcagga gatcagcggc
      601 tgggtcgccc agctcgatca cgccgccgcc gcgatccgcg ccgcgctgcc cgccgtccac
      661 gagctcgcgc tcggcggcac cgcggtcggc accggcctca acacccaccc ggcctacgcc
      721 gtccgcgtcg cggcccggct cgccgagctc accggcctgc cgctggtcac cgcgcccaac
      781 aagttcgccg cgctcgccgg ccacgacgcc ctggtcggcc tgcacggcgc gctcaagcag
      841 ctcgccaccg cgctcaccaa gatcgccaac gacgtgcgct ggctcgccag cggtccgcgc
      901 agcggcctgg gcgagctgcg catcccggag aacgagcccg gcagctcgat catgcccggc
      961 aaggtcaacc cgacccagtg cgaggcgatg accatggtat gtgcccaggt cgtcggcaac
     1021 gacgtcgcga tcgcgatcgg cgccgcgagc ggcaacttcg agctcaacgt ctacaagccg
     1081 ctgatcgccc acaacgccct gcagtcgatc cgcctgctcg gcgacgcctg cgcctcgttc
     1141 gacgtccact gcgcgcgcgg tctcgagccc gaccgcgccg agatcgaacg caagctgcat
     1201 ggcagcctga tgctggtcac cgcgctcgcc ccgcacatcg gctacgacaa ggccgcccag
     1261 gtcgccaagc atgcccacgc ccacggcacg ctcttgcgcg acgccgcgat cgccctgggc
     1321 tacgtcaccg gcgacgactt cgaccgctgg gtccgccccg aggccatggt cggcccggcg
     1381 gccgaacgct gaccgcgccg gctgctatcg gagtgcctcg gtcacgctgc gcgccgtgat
     1441 cgcctggtcg agccagcgct ccagggtcgc gagatcgggg caggcattga tgcgcgcctc
     1501 gtccgtttgg ctcggcgtga gctgtcggcc ggcgagcacg cggcgcagcg ctctgcgggc
     1561 ctcgagcagc tggccttttt cctccccctt caccaggcct tcggcccgtc ccttgtcgat
     1621 cagccgctgc cgtgtctcgg ggctgctttc gagcaaggcc tccaggatgt gctgcctccg
     1681 ggcctcgatc tcgggatcat cgaccggacc gaacttcacc agcagctcct gacgcgtcgg
     1741 catcgtcatc ggaagatatt ctagcatgct cagctcccag tcgagcggcc ggcgcgacgc
     1801 gacccactga ccaaattcgt ccagcgcccg acccgaccga gccaggagga acgggatcag
     1861 ctcgtcgacc agcggtagct cgtttgctgc gatccggaga tcgggatgcg ccagttccgc
     1921 agtgcggatt ctgatgttcg gcgtgtgtgg cagtcaagcg gccagtagtg ttgactacgt
     1981 tcgcggcctg tcgctgacga gtttgggatg ctggctcacg atcggctgga gatctgcagt
     2041 gtgctgtgag tcgcggtgac gcggtgaacg acgaccgtgg cgcggaccgg gcggaacccg
     2101 tcgaactcga cctcgagctc gtcgatgccg tcgaagtgca ggcggtcggc ggtcatcttg
     2161 accgggccga gctggacgtc cttgatcgtg gtgtcgatgc tgccgatc
//
LOCUS       DASVOR010000636         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_64424,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000636 DASVOR010000000
VERSION     DASVOR010000636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64424"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VF516_12615"
     CDS             <1..835
                     /locus_tag="VF516_12615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108565.1"
                     /translation="RVTALDVPPGTARLVASGTGSTKDRKLVWADQGGVWTGPAADPH
                     RKTRAALDVPLRGFLASPDGARAIAVYPDQVFADLHHKRSAEVLMTVQLDGQGARRKA
                     IRDGVAVEWSHDARWLLVQDGASTCIMRASGGQYKCWRGFTAASLSADGRWALALGNR
                     DGSRQAPTRSGKSDRSGKSAAKPTPAPLATRPPPTPTPTPSDGNPPGPDDKPWDNLDE
                     PSDEPEAGEPPPAIDDVSVAVPSGPLSLYRLRLECAFTDRPTLLVKVIDGAAVWVPGS
                     P"
     gene            complement(869..1153)
                     /locus_tag="VF516_12620"
     CDS             complement(869..1153)
                     /locus_tag="VF516_12620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108566.1"
                     /translation="MQMSDNPMVALNHAIATAMVRGPAAGLERLDALAHDARLAEHHR
                     LDAVRGHLLERAGDRAGAQRHYRRAAERTTSVPERNYLVMKAARLDEEPR"
     gene            complement(1364..1762)
                     /locus_tag="VF516_12625"
     CDS             complement(1364..1762)
                     /locus_tag="VF516_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8108567.1"
                     /translation="MKYMLMMNTPGGGPYQIARWPQQDIQAHIAFMKDFAKALQESGE
                     LVAAEGLAGPDQAKRVRAGSDGKPITEGVFPEAKEVLAGYWIVDVDRPERAYEIAARA
                     SAAPGPGGKPLNMAIEVREVMSAPPRDLLE"
     gene            2001..>2443
                     /locus_tag="VF516_12630"
     CDS             2001..>2443
                     /locus_tag="VF516_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108568.1"
                     /translation="MRWLAWVMVASLSGCSLAFVNGPPPQHEQLPAFDCTESRVAPVL
                     DTLFAALQVANFAVAAGSTDQQWSDTFNGHPPINRGPAIPLYAALAALGTVSAYYGYS
                     KTGDCREALEAARNRARGTGVGPQAPLPPPPGVPPGTAVPPPVVLP"
BASE COUNT          299 a         1056 c          745 g          343 t
ORIGIN      
        1 ccgcgtcacc gcgctcgacg tcccgcccgg caccgcgcgc ctcgtcgcca gcggtaccgg
       61 gagtacgaag gaccgcaagc tggtgtgggc cgaccagggc ggcgtgtgga ccggccccgc
      121 cgccgacccc caccgcaaga cccgcgccgc gctcgacgtc ccgctgcgcg gcttcctggc
      181 cagccccgac ggcgcccgcg cgatcgccgt ctaccccgac caggtgttcg ccgacctcca
      241 ccacaagcgg tccgccgagg tcctcatgac cgtgcagctc gacggccagg gcgcccgccg
      301 caaggcgatc cgcgacggcg tcgccgtcga gtggtcgcac gacgcgcgct ggctgctggt
      361 ccaggacggc gccagcacct gcatcatgcg cgcgagcggc ggccagtaca aatgctggcg
      421 cggcttcacc gccgcgtcgc tgtccgccga cggccgctgg gccctcgccc tcggcaaccg
      481 cgacggctca cgccaggctc ccacccggtc cggcaagtcc gaccggtccg gcaagtcggc
      541 cgccaagccg acgcctgcgc ccctcgccac ccggccgccg ccaacgccga cgccgacgcc
      601 gagcgacggc aacccgcccg gccccgacga caagccgtgg gacaacctcg acgagccgtc
      661 cgacgagccc gaggcaggcg agccgccccc cgcgatcgac gacgtcagcg tcgccgtgcc
      721 gagcggcccg ctgtcgctgt accggctccg cctcgagtgt gcgttcaccg atcgaccgac
      781 cctgctggtc aaggtcatcg acggcgccgc cgtctgggtg cccggctcgc cgtagccgcc
      841 gcgcccggtc gcatcgtgca agcgcccgtc accgcggctc ctcgtcgagc cgcgccgcct
      901 tcatcaccag gtagttcctt tccggaacgc tcgtcgtgcg ctccgccgcg cgccggtaat
      961 gccgctgcgc ccccgcgcga tcgcccgcgc gctccagcag gtgcccgcgg accgcatcga
     1021 gccggtggtg ctccgccagc cgcgcgtcgt gcgccagcgc gtcgagccgc tccagccccg
     1081 ccgccggccc gcgcaccatc gccgtcgcga tcgcgtggtt gagcgccacc atcgggttgt
     1141 ccgacatctg caacagcagc ccgtacagcg ccaggatctt ccggcacccc gtcgcccggc
     1201 cagcgctccg ccgccgcgat cagcgcctgc tgcaccgcgt cctccgccgc cgcaaagtcc
     1261 cccgaccgcc gcatcacggc gcccagcacc tgcggcgcca gctcacgcag cagcccctcg
     1321 atcacgccct cggtccgccc ctcggtgcgc ggctcccgtg cgctcattcc agcaggtctc
     1381 gaggcggcgc gctcatcacc tcccgcacct cgatcgccat gttcagcggc ttgccccccg
     1441 gccccggcgc cgccgacgcc cgcgccgcga tctcgtacgc ccgctccggc cggtcgacat
     1501 cgacgatcca gtacccggcg aggacctcct tcgcctcggg aaacaccccc tccgtgatcg
     1561 gcttgccgtc gctccctgcc cgcacccgct tggcctggtc gggcccggcc agcccctcgg
     1621 ccgccaccag ctcccccgac tcctgcagtg ccttcgcaaa gtccttcatg aacgcgatgt
     1681 gcgcctggat gtcctgctgc ggccagcgcg cgatctggta cggcccgccg ccgggcgtgt
     1741 tcatcatcag catgtacttc atgcgcgact ccttctcggt ggttgcgctc cctttggtgc
     1801 gcgttcacgg ggacgtcgga gccgacggcc catcctcgac atccgcgacc gacttttttt
     1861 tcgccgcggt ccctgcgccg ccagcgtatc gccgaccgcc gcgcccgcca aaccggccgc
     1921 ctccggagcc ggctgccgcc acccgcggtg cgatgcctcc tgccttgacc tctccacgac
     1981 gatcggctta tagcctcgcc atgcgatggc tggcgtgggt gatggtcgcg tccttgtcgg
     2041 gttgctcgct cgcgttcgtc aacggcccgc cgccgcaaca cgagcagctc cccgccttcg
     2101 attgcaccga gagccgcgtc gctcccgtcc tcgacacgct gttcgccgcg ctccaggtcg
     2161 cgaacttcgc cgtcgccgcg ggctccaccg accagcaatg gtccgacacg ttcaacggcc
     2221 atcctccgat caaccgcggc cccgcgatcc cgctctacgc cgccctcgct gcccttggca
     2281 ccgtcagcgc ctactacggc tactcgaaga ccggcgactg ccgcgaggcg ctcgaggccg
     2341 ccaggaatcg agcccgaggt accggcgtcg gtccccaggc tcccttgccg cccccgccgg
     2401 gagtgccgcc gggcaccgcc gtccccccgc cggtcgtgct acc
//
LOCUS       DASVOR010000637         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_181663,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000637 DASVOR010000000
VERSION     DASVOR010000637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181663"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF516_12635"
     CDS             complement(<1..414)
                     /locus_tag="VF516_12635"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEX8108569.1"
                     /translation="MSRRWIWIAIALVIGAAAVAMLGDVRALPARLGGFRWSAFAAAL
                     GLALGNYTIRCVRWALYLRRQAVRVPPSVSALVFGAGMSLSITPGKVGDLIKSYLLRE
                     LRGVPATRTAPIVIAERVTDLVALLVLAVAGVAAYG"
     gene            507..890
                     /locus_tag="VF516_12640"
     CDS             507..890
                     /locus_tag="VF516_12640"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8108570.1"
                     /translation="MSKAQGASILVVDDDPEIVTMLTTRLGHRDYKVSSAGDGHRALE
                     LARREKPDLVLLDVMMPGKSGWEVARALKQDPVTQGIKIVMVTAIGEQVNEITSPLYG
                     ADAHVDKPFEFERLERVIGGLLTGH"
     gene            complement(877..>1600)
                     /locus_tag="VF516_12645"
     CDS             complement(877..>1600)
                     /locus_tag="VF516_12645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108571.1"
                     /translation="AHSDAASGDKVHADASSAKTTRPVKHTEPRKPEHKAEVAMAPPA
                     RPAPHHGGRSLQDVKSEATGLYRSKNFGGAAAAINSSLSGFSGDDVKDLKAIAAIYSQ
                     LGKAYAVGMAPGTKPVEAYQALLRASDYDREVGSAYSVDLRDKLVAIAPRAATSYMAA
                     KSFEQAFQAVRKAEELGSRSDDLKIVRQNLEDKAKELLRTARSEIATDPDAAKQKLRQ
                     VQGMVERQNTLWQQAAKLLNGP"
BASE COUNT          230 a          557 c          568 g          245 t
ORIGIN      
        1 gccgtacgcc gcgacgccgg cgaccgcgag caccagcaac gcgaccaggt cggtgacgcg
       61 ctcggcgatc acgatcggcg cggtccgggt cgcgggcacg ccgcgcagct cgcgcagcag
      121 gtagctcttg atcaggtcgc cgaccttgcc gggcgtgatc gacagcgaca tgccggcacc
      181 gaacaccagt gcgctgacgc tcggtggtac gcggaccgcc tggcgccgca ggtagagcgc
      241 ccagcgcaca caccggatcg tgtaattgcc gagcgcgagg ccgagcgctg cggcgaacgc
      301 cgaccaccgg aaaccgccga gccgggccgg caacgcgcgg acatcgccga gcatcgcgac
      361 cgccgccgcc ccgatcacca gggcgatcgc gatccagatc cagcgccgag acaccgctcg
      421 cctccgtatc acacttcccg ctacggtgtg ttgcaaaccc gggccagtgg ttgacctcgg
      481 gaagccgcgc ggcgtagcgt ggatccatga gcaaggcgca gggcgcgagc atcctggtgg
      541 tcgacgacga tccggagatc gtaacaatgc tcacgacgcg cctggggcac cgcgactaca
      601 aggtcagctc tgcgggggat ggccaccgcg cactggagct cgcccggcgc gagaagccgg
      661 acctcgtgct gctcgacgtg atgatgcccg gcaagtcggg ctgggaggtc gcgcgcgccc
      721 tgaagcagga tccggtcacc cagggcatca agatcgtgat ggtcacggcg atcggcgagc
      781 aggtcaacga gatcacgtcg ccgctctacg gcgcggacgc gcacgtcgac aagccgttcg
      841 agttcgagcg gctcgagcgc gtgatcggcg gcctgctcac gggccattga gcagcttggc
      901 ggcctgctgc cagagcgtgt tctggcgctc gaccatgccc tggacctgac gcagcttctg
      961 cttggcggcg tcggggtcgg tcgcgatctc gctgcgcgcc gtgcgcaaca gctccttggc
     1021 cttgtcctcg aggttctgcc gcacgatctt gaggtcgtcg ctccggctgc cgagctcctc
     1081 ggccttgcgc acggcctgga acgcctgctc gaagctcttg gcggccatgt acgaggtcgc
     1141 cgcgcgcggc gcgatcgcca ccagcttgtc gcggagatcc accgaatagg cgctgccgac
     1201 ctcgcgatcg tagtcgctgg cgcgcagcag cgcctggtag gcctcgaccg gcttggtccc
     1261 gggagccatc ccgaccgcgt aggccttgcc gagctgggag tagatcgccg cgatcgcctt
     1321 gaggtccttg acgtcgtcgc cgctgaaccc ggacagcgac gagttgatcg ccgccgcggc
     1381 accgccgaag ttcttgctgc ggtacagccc ggtggcctcg ctcttgacgt cctgcaggct
     1441 gcggccgccg tggtgcgggg cgggccgcgc cggcggcgcc atcgcgacct cggccttgtg
     1501 ctcgggcttc ctgggctcgg tgtgcttgac cggccgggtg gtcttggccg agctggcgtc
     1561 ggcgtggacc ttgtcgcccg aggcggcgtc gctgtgggcc
//
LOCUS       DASVOR010000638         3414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_385786,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000638 DASVOR010000000
VERSION     DASVOR010000638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3414
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385786"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1439
                     /locus_tag="VF516_12650"
     CDS             <1..1439
                     /locus_tag="VF516_12650"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX8108572.1"
                     /translation="QLRLVKHLVVDHQNLVVVGDDDQSIYSWRGADPTNILRFAELFP
                     GAQIVKLEQNYRSSRTILAAANEVIAHNPKRHGKQLWSHHDQGEPITHAVAATAEDEA
                     RWVAAEIARLHHDGRRWSDIAVMYRSNLQAKALEDELRQASAPYVMYGGQQFFERKEV
                     KDVIAYLRVALNVRDDLALRRVINYPARGIGATTVERLVTAAQARHAPLWDALRAAIA
                     GGTEALPGIGTAMPRDDADPAPQDVEVADLRGAARNGIIELVHVVSELASALEAGTDV
                     VSATRTLIEDIRLYDDLRDAAGSAAAAQRRIDNVEGLLGSLGRFAEKQRGRAALAEYL
                     RMLSLETSEEREDSGDRVVLTTLHGAKGLEFPVCFMIGLEEELLPHIRTLQPQVTDVI
                     DADHATDISEERRLCYVGITRAQRKLYLTRACARVQRGRSLPRTPSRFLLEIPDELLE
                     VRDIAEEARQAVPTDEVKSFFASFSLDD"
     gene            complement(1766..2359)
                     /locus_tag="VF516_12655"
     CDS             complement(1766..2359)
                     /locus_tag="VF516_12655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108573.1"
                     /translation="MQLPAYMRITTSPVCLVSFGSLVSLGILGALAAPARAEHKDAGK
                     SRERIAAAAAEAVRTERGGASLTTGELGLTGARAARRGMSLEAGEVAAEVRPYAPDIE
                     RCYLDRLGDVSRAGRLDLTFVIGRDGYVVSLDAAAPGLPARTVRQMAICIREAVDAIH
                     FPERRADTTAVVPYYFQHTNTPGGGPQLSCWNPKGCH"
     gene            2589..>3414
                     /locus_tag="VF516_12660"
     CDS             2589..>3414
                     /locus_tag="VF516_12660"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX8108574.1"
                     /translation="MKQTAILGGLVLAACGSAARSPSTGQTTAADHAATAATEPAPSP
                     AQPADPALGFRLQYTDPGGMWLPQQMTLPFHVENFRNMGVAIDAKTLSDPLASPLNAV
                     VSLGGCTASFVSPDGLIVTNHHCVQQALQINTNRKAGVDLVEDGFMAKTMADEKPAGP
                     AQRVSVAQAFKDVTREMRDGLDQIKDPVARREEAEKRLKTLIASCEKDRPSIRCTVRA
                     YFGTAMYIQTENLEIRDVRLVYAPARSVGDFGGEVDNWHWPRHTGDWSFYRAYVGKD"
BASE COUNT          533 a         1240 c         1169 g          472 t
ORIGIN      
        1 cgcagctgcg gctggtcaag cacctggtcg tcgatcacca gaacctggtg gtggtcggcg
       61 acgacgacca gtcgatctac agctggcgcg gcgccgatcc gaccaacatc ctgcggttcg
      121 ccgagctgtt ccccggcgcg cagatcgtca agctcgagca gaactaccgg tcgtcgagga
      181 cgatcctcgc cgccgccaac gaggtgatcg cccacaaccc caagcgccac ggcaagcagc
      241 tgtggtcgca ccacgaccag ggtgagccga tcacccacgc ggtggcggcg accgccgagg
      301 acgaggcgcg ctgggtcgcg gccgagatcg cgcggctcca ccacgacggc cggcggtgga
      361 gcgacatcgc ggtgatgtac cgctccaacc tccaggccaa ggccctcgag gacgagctgc
      421 gccaggccag cgccccgtac gtgatgtacg gcggccagca gttcttcgag cgcaaggagg
      481 tcaaggacgt gatcgcgtac ctgcgggtcg cgctcaacgt ccgcgacgac ctcgcgctgc
      541 gccgcgtgat caactacccg gcgcgcggga tcggcgccac caccgtcgag cggctggtga
      601 ccgccgccca ggcccgtcac gcgccgctgt gggacgccct gcgcgccgcg atcgccggcg
      661 gcaccgaggc gctgcccggg atcggcacgg cgatgccgcg cgacgacgcg gaccctgccc
      721 cgcaggacgt ggaggtggcg gacctgcgcg gcgccgcgcg caacggcatc atcgagctgg
      781 tccacgtggt gtccgagctc gcctccgcgc tggaggccgg caccgacgtc gtctcggcga
      841 cccgcacgct gatcgaggac atccggctgt acgacgacct gcgcgacgcc gcgggctcgg
      901 ccgccgccgc ccagcgccgg atcgacaacg tcgagggcct gctgggctcg ctcggccggt
      961 tcgccgagaa gcagcgcggc cgcgccgcgc tcgccgagta cctgcgcatg ctgtcgctcg
     1021 agaccagcga ggagcgcgag gactccggcg accgcgtggt gctgaccacg ctgcacggcg
     1081 cgaagggcct cgagttcccg gtctgcttca tgatcggcct ggaggaggag ctcctgcccc
     1141 acatccgcac gctgcagccc caggtcaccg acgtgatcga cgccgatcac gcgaccgaca
     1201 tcagcgagga gcgccggctg tgctacgtcg gcatcacccg cgcgcagcgc aagctgtacc
     1261 tgacccgggc ctgcgcgcgc gtccagcgcg gccgctcgct gccgcgcacg ccgtcgcggt
     1321 tcctgctcga gatccccgac gagctgctcg aggtgcgcga catcgccgag gaggcccgcc
     1381 aggcggtgcc gaccgacgag gtcaagagct tcttcgcgtc gttctcgctc gacgactagc
     1441 tgacccgcgt cgtcgcgatg tgaacgaagc tgagctgaag tcgacgtcga agatttgctg
     1501 gatcggcttt ccgtcttgac gttgtcctcg acgatgacct tgacccgaac gttgacgtcg
     1561 acggcgacga tgacgtctga ctccaccctt gaccttgtac cttgactccg gaggaccttc
     1621 gaggtcgagg tcgacggtgg ggtcgacgtc ctacgtcgcc ctcaacgtca acgttcgggt
     1681 caacctcgac gtcaacccgt ccacgtcaac ccccccgcgt ctcgaatcgc cttcccctct
     1741 tcattcagca acccctgagg ccgacctagt ggcagccctt ggggttccag cagctgagct
     1801 gcggaccgcc gcccggcgtg ttggtgtgct ggaagtagta cggcacgacc gcggtcgtgt
     1861 cggcgcggcg ctcggggaag tggatcgcat cgaccgcctc gcggatgcag atggccatct
     1921 ggcgcacggt cctggccggc agccccggcg cggcggcgtc gagcgacacc acgtagccgt
     1981 cgcggccgat cacgaacgtc aggtcgagcc gtccggcgcg gctcacgtcg ccgagccgat
     2041 cgaggtagca gcgctcgatg tccggcgcgt acggccggac ctccgcggcg acctcgccgg
     2101 cctccaggga catgcccctg cgcgccgcgc gggcgccggt gaggccgagc tcgccggtgg
     2161 tcagcgatgc accgccgcgc tcggtgcgga ccgcctcggc tgccgccgcc gcgatgcgct
     2221 cgcgcgactt gccggcgtcc ttgtgctccg cccgggcggg agcggccagc gccccgagta
     2281 tccccagcga cacgagcgag ccgaacgaga cgaggcacac aggcgaggtc gtgatccgca
     2341 tgtacgccgg cagttgcacc cgccatacca cgcacaacca cgcaggatcg cttggcgata
     2401 ccccgggagg acacaccggt ttgtctaccg ggggagacac gctggacggc gggaagcgtc
     2461 aaccgcggta gacgcggagc cgggcgccgc cgcccgacgc atggaccgga ccgttgtctc
     2521 gcgctcccgt cacggcttcg tgttgaggaa tgttcgttcc agatgcgatt cgcggtatgg
     2581 agtcgggcat gaagcagacg gcaatcctcg gtgggctcgt cctcgcggcc tgtggcagcg
     2641 cggccaggag tccgagcacc ggacagacca cggccgcgga tcacgcggcc accgcggcga
     2701 ccgagccggc cccctcgccg gcgcagcccg cggacccggc gctcggcttc cggttgcagt
     2761 acaccgaccc cggcgggatg tggctgcccc agcagatgac cctgccgttc cacgtcgaga
     2821 acttccgcaa catgggcgtc gcgatcgacg ccaagacgct gtccgatccg ctggcgtcgc
     2881 cgctcaacgc ggtggtgtcg ctcggcggct gcaccgcgtc gttcgtgtcg cccgacgggc
     2941 tgatcgtcac caaccaccac tgcgtccagc aggcgctgca gatcaacacc aaccgcaagg
     3001 ccggggtgga cctcgtcgag gacggcttca tggcgaagac catggccgac gagaagcccg
     3061 ccgggccggc gcagcgcgtg tcggtcgctc aggcgttcaa ggacgtcacc cgcgagatgc
     3121 gcgacgggct cgaccagatc aaggacccgg tggcgcgccg cgaggaggcc gagaagcggc
     3181 tcaagaccct gatcgcctcg tgcgagaagg accgcccgag catccgctgc acggtgcgcg
     3241 cgtacttcgg caccgcgatg tacatccaga ccgagaacct cgagatccgc gacgtccgcc
     3301 tggtctacgc gccggcgcgc tcggtcggcg acttcggcgg cgaggtcgac aactggcact
     3361 ggccgcgcca caccggcgac tggtcgttct accgggccta cgtcggcaag gacg
//
LOCUS       DASVOR010000639         1641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_152179,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000639 DASVOR010000000
VERSION     DASVOR010000639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152179"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(279..1025)
                     /locus_tag="VF516_12665"
     CDS             complement(279..1025)
                     /locus_tag="VF516_12665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEX8108575.1"
                     /translation="MISVRSLCKRHGSREVLSGVTAAVERGETIAIVGPSGGGKSTLL
                     RCLNDLEAFDAGEVEIAGVTLRPAMAGGDPALRRLRARVGMVFQQFNLFPHLTALDNV
                     ALAPRVVKRIERAAAERSARELLARVGLADRAASYPHELSGGQQQRVAIARALAQEPE
                     VMLFDEPTSALDPEMRDDVMEVIRGLARGGMTMLVVTHEMQFAHDIASRVWVIDRGTI
                     AEQGPPAQVVDRPETDVAREFFSRIRRAST"
     gene            complement(1000..>1641)
                     /locus_tag="VF516_12670"
     CDS             complement(1000..>1641)
                     /locus_tag="VF516_12670"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEX8108576.1"
                     /translation="GLVVQFLEAAWVTLELSVLAFLLAVPIGTALAVARVYGARPIAG
                     AARLYIELFRGTPVLLQLFVLYYGLADVISLGPVQAAVLGLGLNYGAYEAEVYRGALL
                     AIPRGQSEAARALGFGPAQTLRHVLLPQALRLALPPMTNDFVSLLKDSSLVSVITVIE
                     LTKRLTIAAVDMRGWLIPGLLCAALYLALSFPLSELARRLERRLARDQRPLAL"
BASE COUNT          253 a          597 c          584 g          207 t
ORIGIN      
        1 cgccccgagc ccgccgccga tcgcagcgcc ggcgtacttg ccgacccact ggccccagcc
       61 ttctccgccg atcacggtgg agaggtcgac gacatcgagt ccgggcaggt cgctgaagtt
      121 gatcgctgga ttgtgcatgc gaaggttcct ttctggtgtt ccgagggttc gacggtccct
      181 cgtgcagccc tcgggccagc gcgactgcgc aactcgatca acgagatcgc cggcgacaag
      241 cgcaaaagcg tgctcatcgc gccagacagc ttgatgactc aggtagacgc gcggcggatc
      301 cggctgaaga actcgcgcgc gacgtcggtc tcggggcggt cgacgacctg cgccggcggg
      361 ccctgctcgg cgatcgtgcc gcggtcgatc acccagaccc gcgatgcgat gtcgtgggcg
      421 aactgcatct cgtgcgtgac caccagcatg gtcatgccgc cgcgcgccag gccgcggatg
      481 acctccatca cgtcgtcgcg catctcgggg tcgagcgcgc tggtcggctc gtcgaacagc
      541 atgacctccg gctcctgggc cagcgcgcgc gcgatcgcga cccgctgctg ctggccgccc
      601 gacagctcgt gcggatacga cgcggcgcgg tcggcgaggc cgacccgggc gagcagctcg
      661 cgcgccgagc gctcggccgc agcgcgctcg atccgcttga cgacccgcgg cgccagcgcg
      721 acgttgtcga gcgcggtcag gtgggggaac aggttgaact gctggaacac catgccgacc
      781 cgcgcgcgca gccggcgcag cgccggatcg ccgccggcca tcgccggccg cagggtcacg
      841 ccggcgatct cgacctcgcc ggcgtcgaac gcctcgaggt cgttgaggca gcgcagcagg
      901 gtcgacttgc cgccgcccga cgggccgacg atcgcgatgg tctcgccgcg ctcgaccgcc
      961 gcggtgacgc cgctcagcac ctcgcggctg ccgtggcgct tacagagcga gcggacgctg
     1021 atcacgcgcc agcctcctct ccagccggcg tgccagctcc gacagcggga agctcagcgc
     1081 gaggtacagc gccgcgcaca gaaggcccgg gatcagccag ccgcgcatgt cgaccgcggc
     1141 gatcgtcagc cgcttggtca gctcgatgac cgtgatcacg ctgaccagcg acgagtcctt
     1201 gagcagcgag acgaagtcgt tggtcatcgg cggcagcgcg agccgcagcg cctggggcag
     1261 cagcacgtgg cgcagcgtct gggccggccc gaagccgagc gcgcgcgccg cctcgctctg
     1321 gccgcgcggg atcgcgagca gcgcgccgcg gtagacctcg gcctcgtagg cgccgtagtt
     1381 gagcccgagg ccgagcaccg cggcctggac cggcccgagg ctgatcacgt cggcgaggcc
     1441 gtagtacagc acgaacagct ggagcagcac cggcgtgccg cggaacagct cgatgtagag
     1501 ccgtgccgcg ccggcgatcg ggcgcgcgcc gtagacccgc gcgacggcga gcgcggtgcc
     1561 gatcggcacg gcgagcagga acgcgagcac cgacagctcg agcgtcaccc acgccgcctc
     1621 gaggaactgc accaccagac c
//
LOCUS       DASVOR010000640         2635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_6265,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000640 DASVOR010000000
VERSION     DASVOR010000640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6265"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..1364)
                     /locus_tag="VF516_12675"
     CDS             complement(93..1364)
                     /locus_tag="VF516_12675"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8108577.1"
                     /translation="MCSAAVALSTTADAKTVVVTADKLVDVATGRQTDRPQVVIVDGR
                     ITQVGKQGDAVPAGATRVDLPGVTLLPGLIDMHTHLTSSPLFGGFNRLLFSDSFWAVI
                     STGHAKATLEAGFTTVRNVGSHDYNDVGLRQAIDGGFVEGPRMWTATYAIGATGGHCD
                     STFFPPSMAQKSPAVIDSPLAGRQMVRQLHKYGATVIKICATGGVFSHGDTPGAQQLT
                     LDEIKAIVDEAHMAGLKVAAHAHGASGIKAAILGGVDTIEHASLVDDEGIKLAVQKGA
                     YFGMDIYNTDYTQAEGKKNGVLEENLQKDRDIALIQRQNYQKALRAGVKMIFSTDAGV
                     YPHGTNARQFATMVKWGATPLQAIQSATITAAAALGTPGDVGQVKVGSYGDLVGVSGD
                     PLQDVTLLERPVFVMKGGEVVKRATAIPGSK"
     gene            1431..2420
                     /locus_tag="VF516_12680"
     CDS             1431..2420
                     /locus_tag="VF516_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108578.1"
                     /translation="MHMPNPRCRILSLALTLAAASAGCGGSGNSQPPLPIGESNAASV
                     AAEALITLGQNSFSVQLPTGVTGAAAALRALGPGMVQRLTAAATGPQSADGTMTQPCA
                     LGGTETIAVAGTTATLTFDHCAADAGTTVNGTFRFTGQLSSTTSQSTLTVSFNLTITQ
                     GALSFAESGGYTAVIKIAQAPSDGTSEEVTGDSISISLGVGGKLRDQVTLSSFDIGIS
                     QETTSAGQQAEHFAYDIDSSWLKGHVSVMTTQDLEQVVDLIMPHQYPFAGQILISGAN
                     HTRLQITILGDETFTPPAGQGQIELQIDPGTGTFGAPVWTGWSELSAMVMTGP"
BASE COUNT          378 a          929 c          914 g          414 t
ORIGIN      
        1 gccgtgcatg ccgagcaccg cggcgaggga ggccgcggag atcgcggcga gccagggctg
       61 ggtccaggtg gatcgcatca tggtgatctg cctcacttgc ttccggggat ggccgtggcg
      121 cgcttgacga cctcgccgcc cttcatcacg aacaccgggc gctcgagcag cgtgacgtcc
      181 tggagcggat cgccggacac gccgacgagg tcgccgtagc tgccgacctt gacctggccg
      241 acgtcgccgg gcgtgccgag cgcggcggcc gcggtgatcg tcgcggactg gatcgcctgc
      301 agcggggtcg cgccccactt caccatggtc gcgaactgcc tggcgttcgt gccgtgcgga
      361 tagacgcccg cgtcggtcga gaagatcatc ttcacgccgg cccggagcgc cttctggtag
      421 ttctggcgct ggatcagcgc gatgtcgcgg tccttctgga ggttctcctc gagcacgccg
      481 ttcttcttgc cctcggcctg cgtgtagtcg gtgttgtaga tgtccatgcc gaagtaggcg
      541 cccttctgga ccgcgagctt gatgccctcg tcgtcgacca ggctcgcgtg ctcgatcgtg
      601 tcgaccccgc cgaggatcgc cgccttgatg ccggaggcac cgtgggcgtg ggccgcgacc
      661 ttgaggccgg ccatgtgggc ctcgtcgacg atcgccttga tctcgtcgag cgtcagctgc
      721 tgcgcccccg gcgtgtcgcc gtgcgagaac acgccgccgg tcgcgcagat cttgatcacg
      781 gtcgcgccgt acttgtggag ctggcggacc atctggcggc cggcgagcgg gctgtcgatc
      841 accgcggggc tcttctgcgc catcgacggc gggaagaacg tggagtcgca gtggccgccg
      901 gtcgccccga tcgcgtaggt cgcggtccac atccgcgggc cctcgacgaa gccgccgtcg
      961 atcgcctggc gcaggccgac gtcgttgtag tcgtgcgacc cgacgttgcg caccgtggtg
     1021 aacccggcct ccagcgtcgc cttggcgtgg ccggtcgaga tcaccgccca gaacgagtcg
     1081 gagaacagca ggcggttgaa gccgccgaac agcggcgagg aggtgaggtg ggtgtgcatg
     1141 tcgatcaggc cgggcagcag ggtcacaccc ggcaggtcga cgcgggtcgc gccggcgggg
     1201 acggcgtcgc cctgcttgcc aacctgtgtg atccgcccgt cgacgatcac cacctgcggc
     1261 ctgtcggtct gcctgccggt ggccacatcg accagcttgt cggcggtgac caccacggtc
     1321 ttggcgtcgg cggtagtact cagtgcgaca gcggcgctgc acacgagggc gaggacagtt
     1381 gcgcgcataa gctctccagg gaagcgccgc gaggctatca tggcggcatc atgcacatgc
     1441 cgaatccgcg ctgccggatc ttgtccctgg ccctgaccct ggcggcagcc tctgcaggct
     1501 gcggcggctc cgggaactcc cagccgccgc tgccgatcgg cgagagcaac gcggcgtcgg
     1561 tcgcggccga ggcgctgatc accctcggac agaactcgtt ctccgtgcag ctgcccaccg
     1621 gcgtcaccgg cgccgcggcg gcgctgcgcg cgctcggtcc cggcatggtg cagcggctga
     1681 ccgcagctgc gaccggcccg cagagcgccg acggcacgat gacccagccg tgcgcgctgg
     1741 gtggcacgga gacgatcgcc gttgccggca cgaccgccac gctcacgttc gaccactgcg
     1801 cggcggacgc cggcaccacg gtcaacggca ccttccggtt cacgggccag ctgtcgagca
     1861 ccacgagcca gagcacgctg accgtgtcgt tcaacctcac gatcacgcag ggggcgctga
     1921 gcttcgccga gtcgggcggc tacaccgccg tgatcaagat cgcccaggcc ccgtcggacg
     1981 gcacctcgga agaggtgacc ggagacagca tcagcatctc gctcggcgtc ggcggcaagc
     2041 tgcgcgatca ggtcacgctg tccagcttcg acatcggcat ctcgcaggag acgacctcgg
     2101 cgggtcagca ggccgagcat ttcgcctacg acatcgacag ctcctggctg aagggccacg
     2161 tcagcgtcat gaccacccag gacctcgagc aggtcgtcga cctcatcatg ccgcaccagt
     2221 acccgttcgc cggccagatc ctgatcagcg gcgccaacca cacccgcctg cagatcacga
     2281 tcctcggcga cgagaccttc acgccgcccg ccggtcaggg ccagatcgag ctccagatcg
     2341 atccgggcac cggtacgttc ggcgctccgg tctggaccgg ctggtcggag ctgtccgcca
     2401 tggtgatgac cgggccctag cccgacggtg cgggtcctgc atcgccggag gggatgcagt
     2461 cccgccggtc ccgcctccct ctcgtcgtct ctctgctgag cgcgggcccc gcggcagctc
     2521 agcccgaggc ggggtccggc gatccactcg gtccggtgcg ctgctacgcg accgcgacgc
     2581 gggattcgct tgcgccgatg gagcaggccc ggcagctgtg catcggcgcc gtcga
//
LOCUS       DASVOR010000641         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_94259,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000641 DASVOR010000000
VERSION     DASVOR010000641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94259"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..960
                     /locus_tag="VF516_12685"
     CDS             157..960
                     /locus_tag="VF516_12685"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8108579.1"
                     /translation="MAKTSQRIALPGGALAHDTGASAGALGLLGAVAVFALAIVCFVA
                     ESYFLAMLLVSIALLIYVAGGEIKKLVQSSLTVFFSSRHVVENAVHLSNTVAALRKAL
                     YMKRDDQGNIKAGPIEEGTPIKLPDNPLVRELKAVLRHKKGEESQEYTEYVAHHYYVD
                     CRERYDHFHAHLEFVANVMPLFGLIGTVIGLIGMFDRLGSNTSVENLAPELAISLQCT
                     LFGAILASVYLSAASRFEQRIKALEYDYEILVHALDVLVRHNAHIEVQA"
     gene            957..1649
                     /locus_tag="VF516_12690"
     CDS             957..1649
                     /locus_tag="VF516_12690"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX8108580.1"
                     /translation="MRHRRRPAGKKSHGALWSIIYIDLMTQIMAFFVILWSIEHGARV
                     TQTPSLGTGVGMGDQTARMVDLPGDVLFASGQTQLASDGKAILDQLFGDADSGVLSFD
                     QGGLAQRKLVIHGHTDDVGIKDANFQLGYQRAYAVYQEIRRHSKEVPDHVILCTHADN
                     TPARSLPPSPSERSETGGGGPDGSAGGAGGRAPRGIDRTIADEARAKNRRITIEDLVV
                     ASKPGEPGPGTR"
BASE COUNT          283 a          650 c          610 g          255 t
ORIGIN      
        1 ccgacaccgc gccggtgcgc tcgatgtggc ggcggctcgc cctggccggc gcggtcgccg
       61 ccgggctcgg cgcggccgcg ctcgcggtga tcgatgccat ccgctacacg tacgacccgc
      121 agccaccccc gccccagacc acggagaggc ccgatcatgg caaagactag tcagaggatc
      181 gcactgcccg gcggcgcgct cgcccacgac accggcgcca gcgccggcgc gctcgggctg
      241 ctgggcgccg tcgcggtgtt cgcgctcgcg atcgtgtgct tcgtcgccga gagctacttc
      301 ctcgccatgc tgctggtgtc gatcgcgctg ctgatctacg tcgccggcgg cgagatcaag
      361 aagctcgtgc agtcgtcgct caccgtgttc ttctccagcc gccacgtggt cgagaatgcg
      421 gtccacctga gcaacacggt cgccgcgctg cgcaaggcgc tgtacatgaa gcgcgatgac
      481 cagggcaaca tcaaggccgg gccgatcgag gagggcacac cgatcaagct gcccgacaac
      541 ccgctggtcc gcgagctcaa ggccgtgctg cgccacaaga agggcgagga gagccaagag
      601 tacaccgagt acgtcgcgca ccactactac gtcgattgcc gcgagcgcta cgaccacttc
      661 cacgcccacc tcgagttcgt cgccaacgtg atgccgctgt tcggcctgat cggcacggtg
      721 atcggcctga tcggcatgtt cgatcgcctg ggctcgaaca cctcggtcga gaacctcgcc
      781 cccgagctcg cgatctcgct gcagtgcacg ctgttcggcg cgatcctcgc gtcggtctac
      841 ctcagcgccg cgtcgcggtt cgagcagcgg atcaaggcgc tggagtacga ctacgagatc
      901 ctggtccacg cgctcgacgt gctggtccgc cacaacgccc acatcgaggt ccaggcgtga
      961 ggcaccgccg ccggccggcc ggcaagaaat cgcacggcgc gctgtggtcg atcatctaca
     1021 tcgacctgat gacccagatc atggcgttct tcgtgatcct gtggtcgatc gagcacggcg
     1081 cccgggtcac ccagacgcca tcgctgggca ccggcgtcgg gatgggcgat cagaccgcgc
     1141 ggatggtcga cctgcccggc gacgtgctgt tcgcgtcggg ccagacccag ctggcgtccg
     1201 acggcaaggc gatcctcgac cagctgtttg gcgatgccga cagcggcgtc ctgtcgttcg
     1261 accagggcgg gctggcgcag cgcaagctgg tgatccacgg ccacaccgac gacgtcggca
     1321 tcaaggacgc caacttccag ctcgggtacc agcgcgccta cgcggtgtac caggagatcc
     1381 gccggcactc caaggaggtg cccgaccacg tgatcctgtg cacccacgcc gacaacacgc
     1441 cggcgcgctc gctcccaccg tcgccgagcg agcgcagcga gacggggggt gggggccccg
     1501 acggctccgc cggcggggcg gggggcagag ccccccgtgg gatcgaccgc acgatcgccg
     1561 acgaggcgcg cgccaagaac cgccggatca cgatcgagga cctcgtcgtg gcgagcaagc
     1621 cgggggagcc cgggccgggg accaggtgac cggagcgccg ccgatgatcg ggctcaagag
     1681 cgtggccgcc atcgcggtgt ccgcgctgct ggtggcgctc ggcgccctgc gccacgggct
     1741 cgatccgagc gcgctgcgcc acctcagccc gggcgcgtgc ttcctcaccg gcctggtc
//
LOCUS       DASVOR010000642         2027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_561647,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000642 DASVOR010000000
VERSION     DASVOR010000642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561647"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2027)
                     /locus_tag="VF516_12695"
     CDS             complement(<1..>2027)
                     /locus_tag="VF516_12695"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEX8108581.1"
                     /translation="CSACFINSVDDSMGSILGLAKTEGMLFKYGSGTGSNLSSLRSSR
                     ENLNGGGTASGPVSFMRGFDAFAGAIKSGGKTRRAAKMVILNIDHPDVVDFIDCKAVE
                     EKKAWALIDAGYDGGFNVVGGAYDSVSYQNANHSVRVTDEFMTAVLRDADWHTRAVVD
                     GKKLDTHNARQLMRKMADAAWVCGDPGIQYDTTINDWHPCINTARINASNPCSEYMFL
                     DDSACNLASLNLRKFQRPASPGSGDQNERTGGGDLDVEAFRRACQITIMAMEIIVDNS
                     SYPTERIAENSYRFRPLGLGFANLGALLMSRSLPYDSEPGRAYAAAITALMCGEAYRT
                     SARIAADATGPFPGYADNETPFLRVMRKHRSAVDRIDSAYVPHDLMQAARDAWDGAID
                     LGQRYGFRNGQTTVLAPTGTIAFLMDCDTTGIEPDIAIVKYKRLVGGGLLKIVNHTVP
                     EALARLGYSEAEVEAIVAYIDAHDTIEGAPHLQDEHLPVFDCAFRSSSGTRSIHYMGH
                     IRMMSAVQPFLSGAISKTVNLPTECTVEDIEDAYIQAWKLGLKAIAVYRDGCKRTQPL
                     STNMKQATSDGSKTAELAPAAPAVPSAPVDPLELLAPEERRLVEALRARKSRPAGPPM
                     ANRYKLPDERASFTHKFSIGGHEGYITVGMYEDQSPGEIFVRMAKEGSVIAG"
BASE COUNT          292 a          678 c          705 g          352 t
ORIGIN      
        1 cccgcgatca ccgagccttc cttggccatg cggacgaaga tctcgcccgg cgactggtcc
       61 tcgtacatgc cgaccgtgat gtagccctcg tggccgccga tcgagaactt gtgggtgaac
      121 gaggcgcgct cgtcgggcag cttgtagcgg ttggccatcg gcggcccggc cgggcgcgac
      181 ttgcgggcgc gcagcgcctc gaccagccgg cgctcctcgg gggcgagcag ctccagcggg
      241 tcgacggggg ccgacgggac cgccggcgcg gccggtgcca gctcggcggt cttggagccg
      301 tcgctggtcg cctgcttcat gttggtcgac agcggctggg tccgcttgca gccatcgcgg
      361 tacaccgcga tcgccttgag cccgagcttc caggcctgga tgtaggcgtc ctcgatgtcc
      421 tcgaccgtgc actcggtggg caggttgacg gtcttggaga tcgcgccgga caggaacggc
      481 tggaccgccg acatcatgcg gatgtggccc atgtagtgga tcgagcgggt gccgctcgac
      541 gaccggaacg cgcagtcgaa caccggcagg tgctcgtcct ggaggtgcgg cgcgccctcg
      601 atcgtgtcgt gggcgtcgat gtacgcgacg atcgcctcga cctcggcctc gctgtagccg
      661 agccgggcca gcgcctcggg caccgtgtgg ttgacgatct tgagcagacc gccgccgacc
      721 aggcgcttgt acttgacgat cgcgatgtcg ggctcgatgc cggtggtgtc gcagtccatg
      781 aggaacgcga tcgtgccggt cggggcgagc accgtggtct ggccgttgcg gaagccgtag
      841 cgctggccga ggtcgatcgc gccgtcccac gcatccctgg ccgcctgcat caggtcgtgc
      901 ggcacgtacg cgctgtcgat ccggtcgacc gccgagcggt gcttgcgcat cacgcgcagg
      961 aacggcgtct cgttgtcggc gtagccgggg aacggccccg tcgcgtcggc cgcgatccgc
     1021 gccgaggtgc ggtaggcctc gccgcacatc agcgcggtga tcgcagcagc atacgcccgc
     1081 ccgggctccg aatcgtacgg tagtgaacgg gacatcaaca atgcccccaa gttcgcgaat
     1141 ccgagcccga gcgggcggaa ccggtagctg ttctcggcga tccgctcggt cggatagctc
     1201 gagttgtcga cgatgatctc catcgccatg atcgtgatct ggcaggcgcg ccggaacgcc
     1261 tcgacgtcga gatcgccgcc gcccgtcctc tcgttctggt ccccggaacc tggcgacgcc
     1321 gggcgctgga acttgcgcag gttgagcgac gccaggttgc acgccgagtc atcgaggaac
     1381 atgtactcgg agcacgggtt ggacgcgttg atccgcgccg tgttgatgca cgggtgccag
     1441 tcgttgatcg tcgtgtcgta ctggatgccg ggatcgccgc agacccacgc ggcgtcggcc
     1501 atcttgcgca tcaactggcg tgcgttgtgc gtatcgagct tcttgccgtc gaccaccgcg
     1561 cgggtgtgcc agtcggcgtc gcgcagcacc gcggtcatga actcgtcggt gacccgcacc
     1621 gagtggttgg cgttctggta gctgaccgag tcgtaggcgc cgcccacgac gttgaacccg
     1681 ccgtcgtagc cggcgtcgat cagcgcccac gccttcttct cctcgaccgc cttgcagtcg
     1741 atgaagtcga ccacgtccgg gtggtcgatg ttgaggatca ccatcttggc cgcgcgccgg
     1801 gtcttgccgc cgctcttgat cgcgccggcg aacgcgtcga acccgcgcat gaacgacacc
     1861 ggccccgacg cggtgccgcc gccgttcaag ttctcgcgcg agctgcgcag cgacgacagg
     1921 ttcgaaccgg tgcccgagcc gtacttgaac agcatgccct cggtcttggc cagcccgagg
     1981 atcgagccca tcgagtcgtc gaccgagttg atgaagcacg ccgagca
//
LOCUS       DASVOR010000643         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_502919,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000643 DASVOR010000000
VERSION     DASVOR010000643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502919"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VF516_12700"
     CDS             <1..1378
                     /locus_tag="VF516_12700"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8108582.1"
                     /translation="GPGGIDGDDAPAQPAPADLRLFGAGDADPRAARALRRLAASVGP
                     GDPFLLQPTAGSTGTPKLVRRSYDAFARYARFVGDEVGKLGAPAPPRMLAIGSLTHAF
                     AGHMFALAMRLGAELAVPRRIDTRASLAEVRALDPDLLPMTPRVLAALVRQAQSRAEP
                     RIFGPRARLLISAGGKGNAQSFERLRAEAVEVIEIYGSSEASVVAVTPLGAWRPGCAG
                     VPVGDVELRFAADGELLVRSPGLALGYHGDPDRGGLVGGDGFYRTGDIGRLDEVGYLE
                     VLGRKRDVFSTPEGSNIYPDRIEILLEALDGVDQALVLGDGKSFVTAHLVVRDPPPGE
                     PSLIDCDTDAALYERFGHALMALNGRLELIEQVVAFALYRRPFPDAVYRTVAGAKVAR
                     DRRAFAAAFAAIADRLYARELAGDSPMLVPPKQRRFLDRVASRGAGVPRRVPEVDRGL
                     TRRAGRRS"
     gene            complement(2035..2289)
                     /locus_tag="VF516_12705"
     CDS             complement(2035..2289)
                     /locus_tag="VF516_12705"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8108583.1"
                     /translation="MNAVSERFVGSVRRELLDHVLVLDEDILALLHQYQLYFNESRPH
                     QGIGQRVPTKPVLTSTCPSRSKSRAYSVGFTSTTAGQRDP"
     gene            complement(2455..>2745)
                     /locus_tag="VF516_12710"
     CDS             complement(2455..>2745)
                     /locus_tag="VF516_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108584.1"
                     /translation="LALIVVCATASADTRDRRLTVVEATIPELQAALRSGRIADAPVA
                     ASSRRLMLELGDLTLGAIVTTAGLADRAVCGRCRVTLEEVGGRAPAALPAGA"
BASE COUNT          373 a          989 c          978 g          405 t
ORIGIN      
        1 cgggccggga ggcatcgacg gcgacgacgc gcccgcacag ccggcccccg ccgacctgcg
       61 gctgttcgga gccggggacg ccgatccgcg cgcggctcgg gccctgcgcc ggctggccgc
      121 gtcggtcggt cccggcgatc cgttcctgct ccagccgacg gccggcagca ccgggacgcc
      181 caagctcgtg cggcgatcgt acgacgcgtt cgcgcgctat gcgcggttcg tcggcgatga
      241 ggtcggcaag ctcggcgcgc cggctccgcc gcgcatgctc gccatcggct cgctgaccca
      301 cgcgttcgcc ggccacatgt tcgcgctggc gatgcggctc ggcgccgagc tcgcggtccc
      361 gcgacggatc gatacccggg cgtcgctcgc cgaggttcgc gcgctcgatc ccgacctcct
      421 gccgatgacg ccgcgcgtgc tcgccgccct ggtccgccag gcccagagcc gcgccgagcc
      481 gcggatcttc ggcccgcgcg cccggctgct gatcagcgcc gggggcaagg gcaacgcgca
      541 gtcgttcgag cggctgcgcg ccgaggccgt cgaggtcatc gagatctacg gctccagcga
      601 ggcctcggtc gtcgcggtca cgccgctggg cgcgtggcga ccggggtgcg ccggggtgcc
      661 ggtcggcgac gtcgagctcc ggtttgccgc cgacggcgag ctgctggtcc gcagcccggg
      721 cctcgcgctc ggctaccacg gcgatcccga ccgcggcggc ctggtcggcg gcgacggctt
      781 ctaccgcacc ggagacatcg gccggctcga cgaggtcggc tacctggaag tcctcggccg
      841 caagcgcgac gtgttcagca cccccgaggg ctcgaacatc tatcccgacc ggatcgagat
      901 cctgctggag gcgctcgacg gcgtcgatca ggcgctcgtg ctcggcgacg gcaagagctt
      961 cgtcaccgcg cacctggtcg tccgcgaccc gccgcccggc gagccgagcc tgatcgactg
     1021 cgacaccgac gccgcgctct acgagcggtt cggccacgcc ctgatggcgc tcaacggccg
     1081 gctggagctg atcgagcagg tcgtcgcgtt cgcgctgtac cggcggccgt ttcccgacgc
     1141 cgtgtaccgc accgtggcag gcgccaaggt cgcgcgcgat cgccgcgcgt tcgccgccgc
     1201 attcgccgcg atcgccgacc ggctgtacgc gcgcgagctc gccggcgact cgccgatgct
     1261 cgtgccgccc aagcagcgcc ggttcctcga tcgcgtggcc agccgcggtg ccggcgtacc
     1321 gcgtcgtgtg ccagaagtag atcgcggact cacgcggcgc gccggtcgta gaagttgagc
     1381 agcccgcccg gtcgtcgccg gctcggcgaa ttcgcgggcc tggtcacacc cgaggcgatc
     1441 ctgcgtaccg cggctgatcg cgcgactgct tgggcgagac caagaacagc ctaccttgtg
     1501 gaaggggtac tgcgtacggg cgcagctgga cgttgatggc atggacgcgc cgacgcgtcc
     1561 cccgggcacg gaggatgctg tcgagcggct cccgcgatcg cgcggcgctt gcggtcgagc
     1621 cgggtggacc cgggcgaacc gactgatctc cgcgatcctg tgcgtcgcgg gctgcgcggc
     1681 atcgcatgcc tcgccatcgc cgtcgccgaa caggctccgt cggtgcgagt cgacgctggt
     1741 gcctcgtcgt cgccgatggc gaccagcgag ctcgtcgcgc aagggcccac gaaactcgtg
     1801 tgtacctggc cgacaccgtt ttgctgcgat cccgacccgg acggcgatgg ctgttacacg
     1861 tgcgcgggca atggcacgca gtgcccctag gacctcctag caggacattg aaaaagcgca
     1921 gagatcggac gaactggctt cccgcttact gcattgaagc ggccattggg atcgttcagt
     1981 gcagcggcgg cgctggcgag ccgtgctggc taccgatctc gtccggcagg atcgtcacgg
     2041 gtcacgctgc cctgcggtag tcgacgtgaa gcccaccgag tacgctcgtg acttcgatcg
     2101 gcttggacag gtcgatgtca ggacgggctt cgtcggcacg cgctgcccga tcccctggtg
     2161 cggtcggctc tcgttgaaat acagctggta ctggtggagg agagccaaga tgtcctcgtc
     2221 gagcaccagt acgtggtcga gcagctcgcg gcgcacactg ccgacgaagc gctccgagac
     2281 tgcgttcata ttcgagtgcc actcggcaaa cgcatccccc acttggagca cgaacgaggt
     2341 agccggtggt gccgcttgcg ccccccgact cccgccatgt tcccgctcgg atgcgcaagg
     2401 tggccagcac gcacccagac gaggaccggg gggggctggt tgagcgcatc tgccctacgc
     2461 ccccgccggg agcgccgccg gggcccggcc gccgacctcc tcgagcgtga cgcggcagcg
     2521 gccgcagacg gcgcgatcgg ctaggcccgc ggtagtgacg atggcgccga gcgtgaggtc
     2581 accgagctcg agcatcaggc gcctcgagct tgctgccacg ggcgcgtcgg cgatccggcc
     2641 ggagcgcaat gccgcctgca gctcggggat cgtcgcctcc accaccgtga gtctccggtc
     2701 cctggtatcg gcggaggcgg tggcgcacac cacgatgagc gcgag
//
LOCUS       DASVOR010000644         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_122903,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000644 DASVOR010000000
VERSION     DASVOR010000644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122903"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..282)
                     /locus_tag="VF516_12715"
     CDS             complement(<1..282)
                     /locus_tag="VF516_12715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108585.1"
                     /translation="MCPLCFTKADITAARRRARFEGSAVALAGAIATVTPFLAQAAVL
                     RMAGGASHDWVALASGIVAAVCGGSTIVAARARASGGWLMVGALGVVLGA"
     gene            571..>2465
                     /locus_tag="VF516_12720"
     CDS             571..>2465
                     /locus_tag="VF516_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEX8108586.1"
                     /translation="MSHPTARPPEPTTATAKDPVCGMSVTPGKAKGGSATHGGREYWF
                     CNPGCKDRFLADPERYLQPQTATAQDPVCGMSVTPGKAKGGSATHAGREYWFCNPGCK
                     DRFLADPERYLGADHAPAGSSPGSSAASAAASGIFTCPMHPEVRRPGAGDCPDCGMAL
                     EPEVPAVGTEYVCPMHPEVVRREPGSCPICGMALEPRAAALDADNPELRDMTRRFAVS
                     AALTAPLVVQSMAEMLGAPIHGLSPGALAWIQLALATPVVAWAGAPLFVRGVRSIVLR
                     KLNMFTLIAIGIATAYGFSVIALLAGGALPAAFRGHGGAPAVYFEPAAVITTLVLLGQ
                     VLELRARRATGSAIRALLGLTPRTARRIADDGSEHEIPLDRVARGDRLRIRPGEKVPV
                     DGTVLDGRSAVDESMITGEPIPVEKAAGDRVLAGTVNGTGGLVIRAERVGSETLLAQI
                     VRMVGEAQRSRAPIQRLADVVASYFVPAVLACAVVTFAVWAVAGPPPALVYALVNAVA
                     VLIIACPCALGLATPMSIMVGTGRGARAGVLVKNAEALQLMEKVDTLVIDKTGTLTEG
                     KPSLVTVLPLDGNADGDRLLRLAAALERGSEHPLAAAIVEGAEERGLDLPAAQNFQSH
                     TGKGVSG"
BASE COUNT          323 a          963 c          856 g          323 t
ORIGIN      
        1 ggcgccgagc accaccccga gcgccccgac catcagccag ccgccgctcg cgcgcgcccg
       61 cgccgcgacg atcgtcgacc cgccgcacac cgcggccacg atgccgctgg cgagcgcgac
      121 ccagtcatgg ctcgcaccgc ccgccatccg caggacggcc gcctgggcga ggaacggggt
      181 caccgtcgcg atcgcgcccg ccagcgccac cgcgctgccc tcgaacctcg cccgccgccg
      241 ggctgcggtg atatctgcct tggtgaagca cagcggacac atcagccctc ggtccgcggt
      301 agggaccacg gcttcccttc gaaccgtctt gccgcaccct ccgcacacct ccggcgaggc
      361 ttcgatcggt actgccatgg gccgcgggac gcggtgcacg gcgcaagcca gcgccggctc
      421 gccggcgcgc gcaagcattg cgcgaacatc gcacgcgcgc tccgccgatc gcgacacggc
      481 gtgtgtcacg ggccgtgtca ctgcgacgcg agatgtctct ccgggtggag acaggcggcg
      541 tcggccgctt cgcggtagac aatgccctgc atgagccacc cgaccgctcg gccgccggag
      601 ccgacgaccg cgaccgccaa ggatccggtc tgtggcatgt ccgtcacgcc gggcaaggcc
      661 aagggcggca gcgcgaccca cggtggccgc gagtactggt tctgcaatcc gggctgcaag
      721 gacaggttcc tcgccgaccc ggagcgctat ctccagccgc agaccgcgac cgcccaggat
      781 cccgtctgcg gcatgtccgt gacacccggc aaggccaagg gcggcagcgc cacgcacgca
      841 ggccgcgagt actggttctg caatcccggc tgcaaggaca ggttcctcgc cgacccggag
      901 cgctacctgg gcgcagatca cgccccggcc ggttcgtcac cgggctcctc ggccgcctcc
      961 gccgctgcga gcgggatctt cacctgcccg atgcaccccg aggttcgccg tcccggcgcc
     1021 ggcgactgcc ccgactgcgg catggccctc gagcccgagg tgcccgcggt cggcaccgag
     1081 tacgtgtgcc cgatgcaccc cgaggtcgtg cggcgcgagc ccggcagctg cccgatctgc
     1141 ggcatggcgc tcgaaccgcg cgccgcggcg ctcgacgccg acaaccccga gctgcgcgac
     1201 atgacccggc ggttcgcggt gagcgccgcg ctgaccgcgc cgctggtcgt ccagtcgatg
     1261 gccgagatgc tcggcgcgcc gatccacggg ctgtcgcccg gcgccctggc ctggatccag
     1321 ctcgcgctcg cgaccccggt cgtcgcgtgg gccggtgcgc cgctgttcgt gcgcggcgtc
     1381 cggtcgatcg tgctccgcaa gctcaacatg ttcacgctga tcgcgatcgg tatcgcgacc
     1441 gcgtacggct tcagcgtgat cgcgctgctc gccggtggcg cgttgcccgc ggcgttccgc
     1501 ggccacggcg gcgcgccggc cgtgtacttc gagcccgccg cggtgatcac gacgctggtc
     1561 ctgctcggcc aggtcctgga gctgcgcgcc cgccgcgcca ccgggagcgc gatccgcgcg
     1621 ctgctcggcc tcacgccgcg gaccgcacgg cggatcgccg atgacggcag cgagcacgag
     1681 atcccgctcg accgcgtcgc gcgcggcgac cggctgcgga tccgccccgg cgagaaggtg
     1741 ccggtcgacg gcacggtcct cgacggccgg agcgccgtcg acgagtcgat gatcaccggc
     1801 gagccgatcc cggtcgagaa ggcggccggc gaccgcgtcc tcgccggcac ggtcaacggc
     1861 accggcggcc tggtcatccg cgccgagcgg gtcggcagcg agaccctgct cgcgcagatc
     1921 gtccgcatgg tcggcgaggc ccagcgcagc cgcgcgccga tccagcggct cgccgacgtc
     1981 gtcgcgtcgt acttcgtgcc ggccgtcctg gcctgcgcgg tcgtgacctt cgcggtgtgg
     2041 gcggtggccg gccccccgcc cgccctggtc tacgccctgg tcaacgcggt cgccgtcctg
     2101 atcatcgcgt gcccgtgcgc cctcggcctg gcgaccccga tgtcgatcat ggtcggcacc
     2161 ggccgcggcg ccagggccgg cgtgctggtg aagaatgccg aggcgctcca gctcatggag
     2221 aaggtcgaca ccctcgtcat cgacaagacc ggcacgctca ccgagggcaa gccctcgctc
     2281 gtcaccgtgc ttcccctcga cggcaacgcc gacggcgatc gcctgctccg cctcgccgcg
     2341 gcgctcgagc gcggcagcga gcatccgctg gcggcggcga tcgttgaggg cgccgaggag
     2401 cggggcctcg atttgcccgc ggcccagaat ttccaaagtc acacgggcaa gggcgtttcc
     2461 gggaa
//
LOCUS       DASVOR010000645         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_473838,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000645 DASVOR010000000
VERSION     DASVOR010000645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473838"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF516_12725"
     CDS             <1..226
                     /locus_tag="VF516_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEX8108587.1"
                     /translation="PSYLVRARDIVQVRESSQKMARINEALAAVDRRGVPQWIQMDKD
                     NFRGAIAQLPSREDVTLPIREQLIIELYSK"
     gene            358..1374
                     /locus_tag="VF516_12730"
     CDS             358..1374
                     /locus_tag="VF516_12730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEX8108588.1"
                     /translation="MEPTPEQTAFMAKNWRDLIRPRTLEIEERSETYGKFSCEPLERG
                     FGTTLGVALRRILLSSLQGAAITHIAIEGALHEFTSLPGVVEDVTDIILNLKETLFKV
                     EHDKTYTVRLDKQGDGTVTAGDIALVDGVTCLNPHHRICTLSKDGKIAIEMTIATGRG
                     YAPAERHSSELPVGTIAIDALFSPVRKVNFTVTNARVGQQTDYDKLTLEVWTNGSVRP
                     DDGVAYAAKILKEQLNLFINFEEMAEPVEAPRDEAAEKLNENLWRTVDELELSVRSAN
                     CLQNANIKYIGELVQKTESEMLKTKNFGRKSLKEIKELLAEMGLQLGMKLDNWPGPNP
                     LKKP"
     gene            1409..1828
                     /gene="rplQ"
                     /locus_tag="VF516_12735"
     CDS             1409..1828
                     /gene="rplQ"
                     /locus_tag="VF516_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012660847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEX8108589.1"
                     /translation="MRHRNSGRKLGRNASHRAALFNNLVTSLLTYGRIETTEAKAKEL
                     RGFADATIGWGVSVQALVAKGDKATKDERAQIVHARRMARRTVKTPEALDRLFGEIGT
                     HFATRKGGYTRLLKTRVRKGDAAPMAFVELVGLYTPS"
     gene            2216..>3754
                     /locus_tag="VF516_12740"
     CDS             2216..>3754
                     /locus_tag="VF516_12740"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8108590.1"
                     /translation="MIIAAAAAAASCGTEPGELASVNQAVSAPPAIRFAEIHYDNTGT
                     DTGEAIEVSGPAGADVTGWQVILYNGNGGASYDTKPLSGPIPATCGTRGVLVINYPSN
                     GIQNGSPDGMALINASGAVVEFLSYEGVFAATNGPANGLTSTDIGVSEAGTEPIGKSL
                     QRHADGTWVGPIDNTFGTCNDNDATPPPPVVASVTVSPATATVVVGGTQTFTAAAFDA
                     AQHPISGVTFTWTSSDPTIATVNAGGVATGVAAGDATITAAAPNGVSGSASLHVNPAP
                     QPGIPDTRFSELHYDNTGVDTGEAIEIEGPAGLNITGWTIVLYDGTNRTAYSTQTLSG
                     AIPATCGTRGVVVVNYPVNGIQNGSPDGMVLVDAAGHVVEFLSYEGTFTATGGPANGV
                     VSTDIIAQEPSTTPIGQSLQRNRDGTWTGPVDNSFGQCNPDRTIRNNNITFRGRAPSE
                     PALPVGFQSQLVATERTAGNATVNTTFTWSSDTPDIATVDQFGVITALAAGTAVFRVT
                     AADGT"
BASE COUNT          661 a         1348 c         1240 g          505 t
ORIGIN      
        1 cccgtcgtac ctggtgcgcg cgcgcgacat cgtgcaggtc cgcgagtcgt cgcagaagat
       61 ggcgcggatc aacgaggcgc tcgcggccgt cgatcgccgc ggcgtgccgc agtggatcca
      121 gatggacaag gacaacttcc gcggcgcgat cgcccagctg ccgtcccgcg aggatgtcac
      181 gctgccgatc cgcgagcagc tgatcatcga gctctattcc aagtaacccg acccgatagc
      241 tttcctggtg gtcgagccgg tcgcacggac cggcctcgcc gcccgaaatg gcaggcctcg
      301 aaacctgcat ggcgatcggc cgtgatgaaa gcgagccgat ccgaggagat gcaagcgatg
      361 gaaccgaccc ccgagcagac cgccttcatg gccaagaact ggcgcgacct gatccggccg
      421 cgcacgctgg agatcgagga gcgcagcgag acctacggca agttctcgtg cgagccgctg
      481 gagcgcggct tcggcacgac gctgggcgtc gcgctgcgcc gcatcctgct gtcgtcgctg
      541 cagggcgcgg cgatcaccca catcgcgatc gagggcgcgc tccacgagtt cacctcgctg
      601 cccggcgtcg tcgaggatgt caccgacatc atcctcaacc tcaaggagac gctgttcaag
      661 gtcgagcacg acaagaccta cacggtgcgg ctcgacaagc agggcgacgg caccgtcacc
      721 gccggcgaca tcgcgctggt cgacggcgtg acctgcctca acccgcacca caggatctgc
      781 acgctgtcca aggacggcaa gatcgcgatc gagatgacga tcgcgaccgg ccgcggctac
      841 gcccccgccg agcgccactc cagcgagctg ccggtcggca cgatcgcgat cgacgcgctg
      901 ttctcgccgg tgcgcaaggt caacttcacg gtgaccaacg cgcgcgtcgg ccagcagacc
      961 gactacgaca agctcacgct cgaggtctgg accaacggct cggtccggcc cgacgacggc
     1021 gtggcgtacg ccgccaagat cctcaaggag cagctcaacc tgttcatcaa cttcgaggag
     1081 atggccgagc cggtcgaggc gccgcgcgac gaggccgccg agaagctcaa cgagaacctg
     1141 tggcgcacgg tcgacgagct cgagctcagc gtgaggtcgg ccaactgcct gcagaacgcc
     1201 aacatcaagt acatcggcga gctggtccag aagaccgagt ccgagatgct caagaccaag
     1261 aatttcggcc gcaagtcgct caaggagatc aaggagctcc tggccgagat gggcctgcag
     1321 ctcggcatga agctcgacaa ctggccgggc cccaacccgc tcaagaagcc ctagggcccg
     1381 gtaccggaat cgatacgagg aagacgccat gcgacaccgc aactcaggac gaaagctcgg
     1441 ccgcaatgct tcgcatcgcg cggcgctgtt caacaacctc gtgacctcgc tgctgaccta
     1501 cggccggatc gagaccaccg aggccaaggc caaggagctg cgcggcttcg ccgacgcgac
     1561 gatcggctgg ggcgtgtcgg tccaggcgct ggtcgccaag ggcgacaagg cgaccaagga
     1621 cgagcgcgcc cagatcgtcc acgcgcgccg catggcgcgc cgcacggtca agacgccgga
     1681 ggcgctcgac cgcctgttcg gcgagatcgg cacgcacttc gcgacccgca agggcggcta
     1741 cacccgcctg ctcaagaccc gcgtccgcaa gggcgacgcg gcgccgatgg cgttcgtcga
     1801 gctcgtcggt ctgtacaccc cgtcctgacg cgatctgcgt cgagcgccgc gtccggcggc
     1861 ctgcagcgct cgatcggtct cacgcggcct gtgtcgagcg ccgcgccctg tggcgtgccg
     1921 cgctccgccg gtcgcagctg tcttgcgccc cgggcgccgg ctgcgcggtt tccctcggtc
     1981 cgagcgtgac gccggtcgca gtcgctcgat ctcgcgacag ccgcgtctcg caaacgtcat
     2041 cgtgacggcg gcgtgatggc cgtcgatcgc taggaccgcc ggccgatcac cgggcgcagc
     2101 gatcgcttca gcgaatcaca ctttcaatcc cagcatcggc tgatgtacgg tgcgctgatt
     2161 tgtgctgtct ctggccacga gggagcgaat gatgcgcagt gcgattcgaa cgggcttgat
     2221 catcgcggcg gccgctgccg cggcatcgtg tgggaccgag ccgggggagc tggcgtcggt
     2281 caaccaggca gtgtccgcgc cgccggcgat ccgtttcgcc gagatccact acgacaacac
     2341 cgggaccgac accggcgagg cgatcgaggt gtccggcccg gccggcgccg acgtcaccgg
     2401 ctggcaggtc atcctctata acggaaacgg cggcgcgtcg tacgacacga agccgctgag
     2461 cgggccgatc cccgcgacct gcggcacgcg cggcgtcctc gtcatcaact atccgtccaa
     2521 cgggatccag aacggcagcc cggatggcat ggcgctgatc aacgccagcg gcgcggtggt
     2581 cgagttcctg tcgtacgagg gggtgttcgc cgcgaccaac ggccccgcga acggcctgac
     2641 ctcgaccgac atcggcgtga gcgaggccgg caccgagccg atcggcaagt cgctgcagcg
     2701 ccacgccgac gggacctggg tcggaccgat cgacaacacg ttcggcacct gcaacgacaa
     2761 cgacgcgacg ccgcctccgc cggtggtcgc gagcgtcacg gtgtcgccgg cgaccgcgac
     2821 ggtcgtggtc ggcggcaccc agaccttcac ggcggccgcg ttcgacgccg cccagcatcc
     2881 gatctccggt gtgaccttca cctggacgtc cagcgatccg acgatcgcga cggtcaacgc
     2941 cggcggcgtg gcgaccggcg tggcggccgg cgacgcgacg atcacggccg cggcgcccaa
     3001 cggcgtgtcg ggctcggcgt cgctgcacgt caaccccgcg ccccagcccg ggatccccga
     3061 cacccggttc agcgagctgc actacgacaa caccggcgtc gacaccggcg aggcgatcga
     3121 gatcgagggc ccggccggct tgaacatcac cggctggacc atcgtgctct acgacggcac
     3181 caaccgcacg gcctacagca cccagaccct gagcggcgcg atccccgcga cctgcggcac
     3241 ccgcggcgtg gtcgtcgtca actatccggt caacgggatc cagaacggca gccccgacgg
     3301 catggtgctg gtcgacgccg ccggtcacgt cgtggagttc ctgtcctacg agggcacgtt
     3361 caccgcgacc ggcgggccgg cgaacggggt ggtgtcgacc gacatcatcg cccaggagcc
     3421 atcgacgacg ccgatcggcc agtcgctgca gcgcaaccgc gacgggacct ggaccggccc
     3481 ggtcgacaat tcgttcggcc agtgcaaccc cgatcggacg atccgcaaca acaacatcac
     3541 cttccgcggc cgcgccccga gcgagccggc gctgccggtc gggtttcagt cccagctggt
     3601 cgccaccgag cgcaccgccg gcaacgccac ggtgaacacg acgttcacct ggtcgtccga
     3661 cacgccggac atcgcgaccg tcgaccagtt cggcgtgatc accgcgctcg ccgccggcac
     3721 cgcggtgttc cgcgtgaccg ccgcggacgg cacc
//
LOCUS       DASVOR010000646         4464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_444321,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000646 DASVOR010000000
VERSION     DASVOR010000646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4464
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444321"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1196
                     /locus_tag="VF516_12745"
     CDS             309..1196
                     /locus_tag="VF516_12745"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX8108591.1"
                     /translation="MPNLELEPVRPLSTFRRLALGTWRTAYDPSVYGSLTLRMDPALA
                     YIDAFRAATGKRLTISHLMARAVAAAYQRVPDANATLRRGRIYRRKRIGVFFQVAMRD
                     PATGELDLSGATIHDPERKSLAEICDELDARFAAVRADRDRALSGVRRMFERLPQRLI
                     PAVLELTAFASYTLNLDLRWAGVPRDAFGSVMITNIGSLGLEEAYVPLVPYSRVPVLL
                     ALGAIRETPIARDGRVAVEHTMKLCATFDHRVLDGAHAAAMADTLRAWIEDPIAHFDR
                     IPDPVPDPLPSNARRAIRA"
     gene            complement(1210..1761)
                     /locus_tag="VF516_12750"
     CDS             complement(1210..1761)
                     /locus_tag="VF516_12750"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8108592.1"
                     /translation="MNSPPTQHVFGRGSETVRLPICEADAEGMLQVPTIADLIPVRRI
                     MTRDVVCAREDLDVGALLDLMVRRRIGCVPVVDDRGRPIGMVTKTDLVEHQLAAMMHR
                     GPAVATAGALMMPLAITLDEHATVSHAAAMMSVEDFHHVAIVSDGGALIGVVSTMDIV
                     RWLAANDGVTAGDIAAVATLPAL"
     gene            complement(1898..2569)
                     /locus_tag="VF516_12755"
     CDS             complement(1898..2569)
                     /locus_tag="VF516_12755"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX8108593.1"
                     /translation="MTTARKTDPDLQKSVIQELAWDRRIDETAIGVSVHHGVVTLNGV
                     VGSWAEKHAAEDAAHRVAGVLDVANDIEIRPVWSTRRSDADIAEAVRHALEWDVFVPE
                     RRIQSTVSDAGHVTLTGAVATLQQRDDAERAVRNLDGVRLVTNLIAIEAPRVAPGELR
                     SQIKAALERHVAREADRIAIDVDDHAVVLSGKVASWRERQAVIGAAKGTPGVQRVEDR
                     LEVAS"
     gene            2746..3546
                     /locus_tag="VF516_12760"
     CDS             2746..3546
                     /locus_tag="VF516_12760"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8108594.1"
                     /translation="MSTTLIHRAEARLRGFHDEGHGFDVFGLHPPTLRRTLALGGRLC
                     DRYFRTRSYGVENIPREGPAILVANHGGALPIDAALVVLDVDRATSRIVRPIADRFIP
                     SLPLISTWFARTGAVVGTRANVARLLARGELIAIWPEGAAGIAKPVHDRYQMRTWQVG
                     HAELAIRHRAPIIPVAILGSEEAWPVLGRIRGFHRFGAPFLPVPAVPFPLPLRHQIHY
                     GAPIALHDGLDPSAADDPCVIAHAATRVRLALAALLHDARQARAGRLA"
     gene            3543..>4464
                     /locus_tag="VF516_12765"
     CDS             3543..>4464
                     /locus_tag="VF516_12765"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8108595.1"
                     /translation="MNVLVTGATTPLGRAIVDRLLAAREVGHVLAVGAEHWGPGLPSD
                     GARFHYEQIDLTRPRSVHDLLYGPARALGIDTVLHGVLHRAAGDRGARVHAINVDATR
                     LLVQACAHHPQIRRFVLRSGGEVYAIRADEPNLLDEDHPLAFDAGAPQWLRDRVEADL
                     VTCAQIGTSGLGTSGLAIAVLRCAEILAAGTGSQLWDYLGSRICLRPLGFDPMINVLS
                     VDDALDAVARALASDARGVFNIPGFDTLPLSGLIALAGRRDVPVPGPLLAPLYRLRTR
                     TVGFEFRYDLNARRFHTGGILDGTRARAALG"
BASE COUNT          554 a         1747 c         1534 g          629 t
ORIGIN      
        1 gccagcgcca gcgcggcgag cgcgatcggg cagatcaggc aggcgagccg cagccagccc
       61 ggccagccgc ggaacgtggt gcgctcgtgc ttggagaccc gccagggaac cgagagcgtc
      121 ctcatgactc cctggacagc atgcggcgtg ccggggtggt gcccggaggc cgaggccgag
      181 gccgagatga gatgagacgg cgtggccgcc cgacgcacgc aacggctgcg ccgcgccggg
      241 tcgacgccgc acgcgctgcg cggtcgtccg ggcgccgccg aggcacgcgc ggtgcatcga
      301 ccgcggagat gcccaacctc gagctcgaac cggtccgccc gctctcgacg tttcgccggc
      361 tcgcgctcgg cacgtggcgc acggcgtacg accccagcgt gtacggcagc ctcacgctgc
      421 ggatggaccc cgcgctggcg tacatcgacg cgtttcgcgc cgcgaccggc aagcggctga
      481 cgatcagcca cctcatggcg cgcgcggtcg cggcggcgta tcagcgcgta cccgatgcca
      541 acgcgacgct gcgccgcggc cggatctacc ggcgcaagcg gatcggcgtg ttcttccagg
      601 tcgcgatgcg cgacccggcg accggcgagc tcgacctgtc gggtgcgacg atccacgacc
      661 ccgagcgcaa gtcgctcgcc gagatctgcg acgagctcga cgcccggttc gccgcggtcc
      721 gcgccgatcg cgaccgcgcg ctgtccggcg tgcggcggat gttcgagcgc ctgccgcagc
      781 gcctgatccc ggcggtgctc gagctcaccg cgttcgccag ctacacgctc aacctcgacc
      841 tgcgctgggc cggcgtgccg cgcgacgcgt tcggctcggt gatgatcacc aacatcggct
      901 cgctcggcct cgaggaggcc tacgtgccgc tcgtgccgta cagccgggtg ccggtcctgc
      961 tcgcgctcgg cgcgatccgc gagaccccga tcgcgcgcga cggccgggtc gcggtcgagc
     1021 acacgatgaa gctgtgcgcg acgttcgacc accgcgtgct cgacggcgcg cacgccgccg
     1081 cgatggccga cacgctgcgc gcgtggatcg aggatccgat cgcccacttc gatcgcatcc
     1141 ccgatccggt ccccgatccg ctgccgagca acgcgcggcg cgcgatccgc gcctgactgc
     1201 ctacagcgcc tacagcgccg gcagcgtggc gaccgccgcg atatcgccgg cggtgacccc
     1261 gtcgttggcg gccagccagc gcacgatgtc catcgtcgag accacgccga tcagcgcgcc
     1321 gccgtcggac acgatcgcca cgtggtggaa gtcctcgacc gacatcatcg ccgccgcgtg
     1381 cgagaccgtc gcgtgctcgt cgagcgtgat cgcgagcggc atcatcaggg ccccggcggt
     1441 ggccaccgcc ggccctcggt gcatcatcgc ggcgagctgg tgctcgacga ggtcggtctt
     1501 ggtgaccatg ccgatcggcc ggccgcgatc gtcgaccacc ggcacgcagc cgatccggcg
     1561 gcgcaccatc aggtcgagca gcgcgccgac gtcgaggtcc tcgcgcgcgc acaccacgtc
     1621 gcgggtcatg atccggcgca ccgggatcag gtcggcgatc gtcgggacct gcaacatccc
     1681 ctctgcgtcg gcctcgcaga tcggcaggcg cacggtctcg ctgccacggc cgaacacgtg
     1741 ctgggtcggc ggcgaattca tcgtagtctg catgggtccc tccaccggag gatcgagcaa
     1801 gccgcgggcc agcgcgaccg gagtctcgtc gcgtggattc gcgcgtaccg cgcacagatc
     1861 gcggcgcccc ccgcaggtcc tgcgcgtttc tcgtccgcta cgacgcaacc tccaggcgat
     1921 cctcgacccg ctgcactccc ggcgtcccct tggccgcgcc gatcacggcc tggcgctcgc
     1981 gccacgacgc gaccttgccg gacagcacca ccgcgtgatc gtcgacgtcg atcgcgatgc
     2041 gatcggcctc gcgggcgacg tggcgctcga gcgccgcctt gatctgcgac cgcagctcgc
     2101 ccggcgccac ccgcggcgcc tcgatcgcga tcaggttggt caccagccgg acaccgtcga
     2161 ggttgcggac cgcgcgctcg gcgtcgtcgc gctgctgcag cgtcgccacc gcgccggtca
     2221 gcgtgacgtg gccggcgtcg gacaccgtcg actggatccg gcgctcgggc acgaacacgt
     2281 cccattccag cgcgtggcgg accgcctcgg cgatgtcggc gtcgctgcgc cgggtgctcc
     2341 acaccggcct gatctcgatg tcgttggcga cgtcgagcac gccggcgacc cggtgcgcgg
     2401 cgtcctcggc ggcgtgcttc tcggcccagc tgccgaccac gccgttgagc gtcacgacac
     2461 cgtggtgcac cgacacaccg atcgcggtct catcgatccg gcgatcccag gcgagctcct
     2521 ggatcacgga cttctgcaga tcggggtcgg tcttcctcgc ggtcgtcatg cccagcgctg
     2581 attgcatcgc ccgttccacg gcctgcccgg cctgcgcttt gcgccatcgc cgcaagcctc
     2641 gcgcgcgacc ttgccggctc gcgcagatcc tgcgggtctc ggcgcgccga cccgtgcgtc
     2701 gcgctggcgc gtggtgtgca gcgtttccgc acgaggaggg ctccgatgtc gaccaccctg
     2761 atccaccgcg ccgaggcccg acttcgcggc ttccacgacg agggccatgg cttcgatgtg
     2821 ttcgggctcc acccaccgac cctgcgccgc accctggcgc tcggcggccg gctgtgcgat
     2881 cgctacttcc gcacccggtc gtacggcgtc gagaacatcc cgcgcgaggg cccggcgatc
     2941 ctggtcgcca accacggcgg cgcgctgccg atcgacgccg cgctggtcgt gctcgacgtc
     3001 gaccgcgcga ccagccggat cgtgcggccg atcgccgatc gcttcatccc gtcgctgccg
     3061 ctgatcagca cgtggttcgc gcggaccggc gcggtggtcg gcacccgcgc caacgtcgcg
     3121 cgcctgctcg cgcgcggcga gctgatcgcg atctggcccg agggcgcggc cgggatcgcc
     3181 aagccggtgc acgaccgcta ccagatgcgg acctggcagg tcggccacgc cgagctcgcg
     3241 atccgccacc gcgcgccgat catcccggtc gcgatcctcg gctccgagga ggcgtggccg
     3301 gtgctcggcc ggatccgggg gttccaccgg ttcggcgcgc cgttcctgcc ggtgcccgcc
     3361 gtgccgttcc cgctgccgct ccgccaccag atccactacg gcgcgccgat cgcgctgcac
     3421 gacgggctcg acccttcggc ggccgatgat ccgtgcgtga tcgcgcacgc cgcgacccgg
     3481 gtgcggctcg cgctggcggc gctgctccac gacgcgcggc aggcccgcgc agggaggctg
     3541 gcatgaacgt cctggtcacc ggcgcgacca ccccgctcgg tcgcgcgatc gtcgaccgcc
     3601 tgctcgccgc gcgcgaggtc ggccacgtgc tcgcggtcgg cgccgagcac tggggccccg
     3661 gcctgccgtc ggacggcgcg cggttccact acgagcagat cgatctgacc cggccgcgct
     3721 cggtccacga cctgctctac ggcccggcgc gggccctggg gatcgacacc gtgctgcacg
     3781 gcgtgctcca ccgcgcagcc ggcgaccgcg gcgcgcgcgt ccacgcgatc aacgtcgacg
     3841 cgacccggct gctggtccag gcctgcgcgc accatcccca gatccgccgg ttcgtcctgc
     3901 gcagcggcgg cgaggtctac gcgatccgcg ccgacgagcc caacctgctc gacgaggacc
     3961 acccgctggc gttcgacgcc ggcgccccgc aatggctgcg cgaccgggtc gaggccgacc
     4021 tcgtcacctg cgcccagatc gggacgtctg gtctcgggac ctccggcctc gcgatcgccg
     4081 tcctgcgctg cgccgagatc ctcgccgcgg gcaccggcag ccagctgtgg gactacctcg
     4141 gctcgcggat ctgcctgcgc ccgctcggct tcgatccgat gatcaacgtg ctgtcggtcg
     4201 acgatgcgct cgacgcggtc gcccgcgccc tggcctcgga cgcgcgcggc gtgttcaaca
     4261 tccccggctt cgacaccttg ccgctgtcgg gcctgatcgc gctcgccggc cgccgcgacg
     4321 tcccggtccc cggcccgctg ctcgcgccgc tgtaccggct gcgcacccgg accgtcgggt
     4381 tcgagttccg ctacgacctc aacgcgcgcc ggttccacac cggcggcatc ctcgacggca
     4441 cccgcgcgcg cgccgcgctc ggct
//
LOCUS       DASVOR010000647         2115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_356863,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000647 DASVOR010000000
VERSION     DASVOR010000647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356863"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..504
                     /locus_tag="VF516_12770"
     CDS             184..504
                     /locus_tag="VF516_12770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8108596.1"
                     /translation="MFDTLEMSLTVLEQLAPVETKIRQRRKSLADEIGRAAESVALNV
                     SEARQRAGLDRADLFRRAAGSASELTTALRIARARGYITAADFAAVDAALDRVRAMLW
                     RLTH"
     gene            538..1722
                     /locus_tag="VF516_12775"
     CDS             538..1722
                     /locus_tag="VF516_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108597.1"
                     /translation="MLGLARFVPVKNGGDLSVLAELCQQASEPGVNFGALSRGLTKQK
                     RRWLECVPAIAGRALRDKLALLARFGVEELGGEDGIVQRTDECLERAFGVAMPGLRDS
                     IAEWIQGVSGATDVTVSLIESVVVEPYLAERPGLITRARHRSARQRYLDAVIRSARRV
                     RPLHAVSHQLFTHTPDLTLASVYVKPPAFTGSYDEPLFEGIPLDRVVSLPRAWQSSVI
                     WEPARRSRSCGFRNLHARISGTRTPGGEQALDEGWPDWRDPRFQQEPLPHPSVVPDVP
                     PPPSHRVRVRIAKLLALVLPKLTDDDLRMFTPRSQYVRDHILVDALEEDPTVAERLEL
                     RHVPLGLLALKGYCVWAQLATFCSVVKWRRERLELDIGLLAAGCVPRRSDRHVPAEAE
                     QR"
     gene            complement(1922..>2115)
                     /locus_tag="VF516_12780"
     CDS             complement(1922..>2115)
                     /locus_tag="VF516_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108598.1"
                     /translation="EVFDALAILAAGSWEPSAVRRGHAAVIALSREMDDGRRLRLGRL
                     GFSTADAARLSSRHTRNFM"
BASE COUNT          341 a          719 c          701 g          354 t
ORIGIN      
        1 gctctcgcgc accgtccctt gccgagcgcc gaaccgaccg acgtgacgcg agtggcgccc
       61 ccacagctgg atccatcgac gagtcgcggg tcgcgctgcc cgcgctcgtc acgggacatg
      121 cgctccgcgg cgcatcgggc gacgacgccc ccggtgctgg gggcgaatcc ccaggaggtt
      181 ccgatgttcg atacgctcga gatgtcactc accgtgctgg agcagctcgc gcccgtcgag
      241 accaagatcc gccagcgccg gaagtcgctc gccgacgaga tcggccgcgc cgccgagtcg
      301 gtcgcgctca acgtcagcga agcgcggcag cgggccggcc tcgaccgcgc cgacctgttc
      361 cgtcgggccg ccggctcggc cagcgagctc accacggcgc tgcgcatcgc acgcgcgcgg
      421 ggctacatca ctgcggccga cttcgcggcg gtcgatgctg cgctggatcg ggttcgtgcg
      481 atgctgtggc gcctcaccca ctgagcacat ctcgggcttg ccggcggatc ccgaggcgtg
      541 ctcggccttg cgcgcttcgt cccagtgaag aacggcggag atctctcggt gctcgcagag
      601 ctatgtcagc aagcgagcga gcccggtgtg aatttcggcg cactgtcacg gggcctaaca
      661 aagcagaagc gccgatggct ggagtgcgtg ccggcgatcg ccggtcgcgc gcttcgggac
      721 aagctcgcac tgctcgctcg cttcggagtg gaggagcttg gcggtgaaga cgggatcgtc
      781 caacgcacag acgagtgcct cgagcgggcg ttcggggtgg ccatgcccgg gctccgcgat
      841 tcgattgcag agtggatcca gggcgtcagt ggtgccacgg acgtcacagt gtcgctgatc
      901 gaatccgtgg tcgttgaacc atatctggcg gagcgacccg gtctcatcac ccgggctcga
      961 catcgcagcg ccaggcagcg gtacctcgat gcggtcatcc gttccgcccg acgcgtccga
     1021 ccgctgcacg cggtttcaca ccagctgttc acccacacgc ccgatctaac cctggccagc
     1081 gtgtacgtta agccgccagc cttcactggt tcttatgacg agccgttgtt cgagggcatc
     1141 ccactcgatc gggtcgtgtc tctgccgcgc gcgtggcagt catcggtgat ctgggaaccg
     1201 gcaagacggt cgcgctcgtg tgggttccgg aatttacacg cccgaatttc cggaacccgc
     1261 actcccggcg gcgaacaggc actcgacgaa ggttggcctg actggcgaga cccacgcttc
     1321 cagcaggagc cattgccaca cccgtcggtc gtgcccgatg tgccgccgcc accctctcac
     1381 cgagttcgtg ttcggatcgc caagctgctt gcgcttgtgc tcccgaagtt gaccgacgat
     1441 gacctgcgca tgttcacgcc acggtcgcag tacgtgcgag atcacatact ggtcgacgct
     1501 ctcgaggaag accccaccgt cgccgaacgg ctcgagctcc gccacgttcc tcttggtctg
     1561 ctcgccctga aggggtactg cgtatgggcg cagctcgcga ccttctgctc ggtggtgaag
     1621 tggcggcggg aacgcttgga actcgacatc ggtctcctgg cagccgggtg cgtaccacgg
     1681 cggtctgacc gtcacgttcc agccgaggca gaacagaggt gaggaagcaa ccgtgcaagg
     1741 gtttcggcgg ggaactttcg aaactggttc cacagcccac cttccaatat ggtctccagc
     1801 acaggtacga gcgcctcggg ccgagcgtcg aggtcgatct tgcgctcgcc agtgtcccag
     1861 gtccatatcg gaaccacgcg aggcatgcgc caccattagc acgccgtccg tgcgattcga
     1921 actacatgaa gttccgcgtg tgccgcgacg acagccgggc ggcgtcggcg gtggagaacc
     1981 cgaggcggcc gaggcggagc cggcggccgt cgtccatctc gcgcgacagg gcgatcacgg
     2041 cggcatggcc gcgccgcacg gccgacggct cccagctgcc ggcggcgagg atcgcgaggg
     2101 catcgaacac ctcct
//
LOCUS       DASVOR010000648         5775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_94344,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000648 DASVOR010000000
VERSION     DASVOR010000648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94344"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VF516_12785"
     CDS             complement(<1..856)
                     /locus_tag="VF516_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108599.1"
                     /translation="MLSATACDPHRKAAEPEDEDEDEQVITTAAGSCGVERWAVKTGT
                     DTTANLVNLTPQDTTIAAMRALAMPSSIPSTTRIPGSAETQAWRISATLTLFKLETDS
                     DFHLVLSDASGATMIVEIPSPSCDAGSVWSTQISHSRNAMTAKFTPSGSFQTANVPVI
                     VTGVGMFDFAHGQTGAAPNQIELHPVLDICFPGSSVSGCATSGDFTLSAPASLSTAAG
                     ASGSATISTAGSGGFTGTVALAVSGVPAGASSSLSPASVAAGGSSTLTLSAGTATPGS
                     YAVTVTGTS"
     gene            1498..1629
                     /locus_tag="VF516_12790"
     CDS             1498..1629
                     /locus_tag="VF516_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108600.1"
                     /translation="MRTRQVAPTILRALDLDPAELQAMRRDGTPVLPRLFVGRGPAR"
     gene            complement(1633..2901)
                     /locus_tag="VF516_12795"
     CDS             complement(1633..2901)
                     /locus_tag="VF516_12795"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8108601.1"
                     /translation="MKGILASLTWLALGALDAGCSARMADPLDTAAPTPIRHVVVVVK
                     ENHTFDNYFGSFPGADGIAQIPTAAGLVTPPHAPDVTPRDLCHNHRCALTDYASGAMN
                     GWDQVSGASTGGDNLMYAQYTEADIPNYWQYARHFTLGDRFFANVLGPSFPGHTFVLA
                     AQAGWASGNPDINIDHPYWGCDQDASARVTVEDPKVGAPAQVFPCFDIPSLPDVLPAG
                     VNWRFYGSNFYVLGEVWSMFNAIKPIRMGPGWAHVVNASTFDLDVDHGALPEVSWLVD
                     QDLDDEHPGYGSVCLGENWTVGHLNHLMNSPLWKDTAIVFTMDDFGGWYDHVAPPRQY
                     GLDPASPYGLGFRLPLIVISPYARPGFVFHEVSEQASIPRFIERVFGAPALSTLDPAA
                     QDGQANDLLGAFDFTQAPLPPLPLPLRTCM"
     gene            complement(3008..4543)
                     /locus_tag="VF516_12800"
     CDS             complement(3008..4543)
                     /locus_tag="VF516_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108602.1"
                     /translation="MAVPSDQDIILSLGEDLPIGLWVARAPRGELVYANHTFAEIMGT
                     MPIAGVRVGAFAAPHGVHTRDGAPYPEHLMPFTRAIAERRVIVADDLTIHRRDGKRVE
                     VRAVARPVGDPITHVIVAFWDISREVEAERARAETEQRLRRAQRLEAIGTLAGGIAHD
                     FNNLIFGIKMIAAELAMSEPDPKRREALKLIDDITERSAALTRSLLGFARRGKNRAAP
                     VSVNDLVTSMSELLTRSLPGVTLTFEIEASDRGTVVGDPTQLEQVIMNLVLNARDAVQ
                     SSGRVVVRTRDHGAPGDPGTARFVVLEVKDDGPGIPAEIRDRVFDPYFTTKITGPERG
                     TGLGLATVLGITESHGGSVEIDAGLGGRGTTMRLMLPAASKPPPPRSHPTVGELPRGS
                     GMILVVDDDPMVRKVVSSSLGVLGYQTVEAGGGIEAVQIYRERHDEIRGVVLDMVMPG
                     MTGRATYLALREIDPDVPVLLMSGHTLNESVQEILDLGVRNFVSKPYSIGALAKAMAE
                     LVP"
     gene            complement(4565..5098)
                     /locus_tag="VF516_12805"
     CDS             complement(4565..5098)
                     /locus_tag="VF516_12805"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF892 family protein"
                     /protein_id="HEX8108603.1"
                     /translation="MDTFRADIENIRRRALEKMDDGAVTASYRADRGKVIGVLNEVLA
                     TETVCTLRYRSHYFMARGIHSSGVEDEFLEHAREEQDHADRVAKRITELGGKPNLDPE
                     GLATRSHAQYGEGDDLEEMIKEDLVAERIAIATYSEIVRWLGSDDPTTRRMMEEILAK
                     EEEHADDMAKLLSRVRK"
     gene            5218..>5775
                     /locus_tag="VF516_12810"
     CDS             5218..>5775
                     /locus_tag="VF516_12810"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX8108604.1"
                     /translation="MLRWPGNLVHFLWLVLVVTACGVMYGVLRLAIFLFVWGPRRTPS
                     LGTLRGVILRWAMTALGATFIKLGQVMSTRPDLFEPEIIAQLRRLQDRLPPFPFHRVR
                     ATVEAELGRPLVELFADFEPAPVAAASVAQVHRAHLRDGREVAVKVLRPGVRRQVERD
                     QAILIGFARLIAIHPTWRRSDPVGHT"
BASE COUNT          846 a         2020 c         2002 g          907 t
ORIGIN      
        1 cgctggtgcc ggtcaccgtc accgcgtagc tgcccggggt cgcggtgccc gcggacaggg
       61 tcaaggtcga gctgccgccg gccgcgacgc tggccggaga caggctgctc gacgcgccgg
      121 cgggcacacc gctcaccgcc agcgcgaccg tgcccgtgaa ccctccgctg cccgcggtcg
      181 agatcgtcgc gctgcccgac gccccggcgg cggtggacag cgaggccggc gcggacagcg
      241 tgaagtctcc cgacgtcgca cagccagaga ccgagctccc cgggaagcag atatcgagca
      301 ccgggtggag ctcgatctgg ttgggggccg cgccggtctg cccgtgcgcg aagtcgaaca
      361 tgccgacgcc ggtcacgatc accggcacgt tggcggtctg gaagctgccg ctcggcgtga
      421 acttggcggt catcgcgttc cgcgaatgcg agatctgggt gctccacacg ctccccgcgt
      481 cgcagctcgg cgacgggatc tcgacgatca tcgtcgcccc ggaggcgtcg gacagaacga
      541 ggtggaagtc gctgtcggtc tcgagcttga acagcgtcag cgtggcggag atccgccagg
      601 cctgggtctc cgcgctcccg gggatgcgcg tggtggaagg gatgctcgac ggcatcgcca
      661 gcgcgcgcat ggccgcgatc gtggtgtcct gcggcgtcag gttgacgagg ttcgccgtcg
      721 tgtccgtgcc ggtcttgacc gcccagcgct cgacgccgca gctgccggcg gcggtggtga
      781 tgacctgctc gtcctcatcc tcgtcctccg gctctgcggc cttgcggtgc ggatcgcacg
      841 cggtggccga aagcacaagt ccgaccgtgg ccagtatcat gaaggggcga gttgccgtgg
      901 attcgatcct cacgaggcct ccgcgttcgc tcgaacattg tcaatgaagc gatgcgtgca
      961 cgatcggccg cgaatgctgt gcaggcaatg cgcacatggt taaattacat cgcatcctcg
     1021 cgtcgacgga ttcgcgagcc ggcagcaacg cgaggcaaaa taggacatcg caatgtgtac
     1081 acgcgagcgc gacatcgatg taacggaatc gactcgcaga acgcgcggtt atcacactcc
     1141 cgatcggatc tgcagcggcc cgggcacccg tgatcgaaag ttcatctggc agtggtcgac
     1201 aggatggagc acgcccgcgt acatcggcgc tgcaccgcga tgccggtgcg ggagttccag
     1261 atgcgcattc cacagtcagt tccgcgctgt ctctcttctt gctgatttcc tgttgtccga
     1321 acagcgagcc cgcatcgacc gcccccgaac cggccgtcgg gctgggctcc ggcacggcac
     1381 gatctactcg ctgagcacca agaagttcgc cgagcacggt ggctcgccga cgacgacgcc
     1441 cacgtcgccc tgctggtctc ccacccggcg ctgcggcacg agacggtcga ccggctggtg
     1501 cgcaccaggc aggtcgcccc gaccatcctg cgcgcgctcg acctcgatcc cgccgagctg
     1561 caggccatgc ggcgagacgg cacgccggtc ctgccgaggc tattcgtggg acgcggaccc
     1621 gcgcgctgat cgctacatgc aggtgcgcag cggcagcggc agcggcggca gcggcgcctg
     1681 cgtgaagtcg aacgcgccga gcaggtcatt ggcctgacca tcctgcgccg ccggatcgag
     1741 cgtcgacagc gccggcgcgc cgaacacgcg ctcgatgaac cgcgggatgc tcgcctgctc
     1801 gctgacctcg tggaacacga agccgggccg cgcgtagggc gagatcacga tcagcggcag
     1861 ccggaacccg aggccgtacg ggctggccgg atcgagcccg tactggcgcg gcggggcgac
     1921 gtgatcgtac cagccgccga agtcgtccat cgtgaacacg atcgcggtgt ccttccacag
     1981 cgggctgttc atcaggtggt tgagatggcc gaccgtccag ttctcgccga ggcacacgct
     2041 gccgtagccc gggtgctcgt cgtcgagatc ctggtcgacc agccagctga cctcgggcag
     2101 cgcgccgtga tcgacgtcga ggtcgaacgt cgaggcgttg accacgtgcg cccagcccgg
     2161 ccccatgcgg atcggcttga tcgcgttgaa catcgaccac acctcgccga gcacgtagaa
     2221 gttcgagccg tagaaccgcc agttgacgcc ggcgggcagg acgtcgggca ggctcgggat
     2281 gtcgaagcac gggaacacct gcgccggcgc gccgaccttc ggatcctcga cggtcacccg
     2341 cgcgctggcg tcctgatcgc acccccagta cgggtggtcg atgttgatgt ccgggttgcc
     2401 gctcgcccag cccgcctgcg cggcgagcac gaaggtgtgg cccgggaagc tcggaccgag
     2461 cacgttggcg aagaaccggt cgccgagcgt gaagtggcgc gcgtactgcc agtagttcgg
     2521 gatgtcggcc tcggtgtact gcgcgtacat caggttgtcg ccgccggtcg acgcgcccga
     2581 gacctggtcc cagccattca tcgcgccgct cgcgtagtcg gtgagcgcgc agcggtggtt
     2641 gtggcacaga tcacgcggcg tgacgtcggg cgcgtgcggc ggcgtgacca ggccggcggc
     2701 cgtcgggatc tgcgcgatgc catccgcgcc ggggaacgag ccgaagtagt tgtcgaacgt
     2761 gtggttctcc ttgaccacga cgaccacgtg ccggatcggg gtcggcgccg cggtgtcgag
     2821 gggatccgcc atgcgcgccg agcagccggc gtccagcgcg ccgagcgcca gccaggtcag
     2881 cgatgccagg atgcccttca ccgccggaga ggtagccgaa gccggctcgc gaatcaaccg
     2941 gaactgccgg gccagcatgg ccgatcgcag cacgccgcgg cggccacacc agccggcgag
     3001 gcagcgctca cgggaccagc tcggccatcg ccttggccag cgcgccgatc gagtacggct
     3061 tggacacgaa gttgcgcacg ccgaggtcga ggatctcctg gacgctctcg ttcagcgtgt
     3121 gccccgacat cagcagcacc ggcacgtcgg ggtcgatctc gcgcagcgcg aggtaggtcg
     3181 cgcgcccggt catccccggc atgaccatgt cgagcacgac gccgcggatc tcgtcgtggc
     3241 gctcgcggta gatctggacc gcctcgatgc cgcccccggc ctcgaccgtc tggtagccga
     3301 gcacgccgag cgagctggac accaccttgc gcaccatcgg atcgtcatcc accaccagga
     3361 tcattccact gcccctggga agctcgccca ccgtcggatg agatctcggc ggaggcggct
     3421 tgctcgcggc cggcagcatc aatcgcatcg tcgtgccgcg gccgcccagc ccggcgtcga
     3481 tctccaccga gccgccgtgg ctctccgtga tcccgaggac cgtcgccagc ccgagcccgg
     3541 tcccgcgctc gggcccggtg atcttggtcg tgaagtaggg gtcgaacacg cgatcgcgga
     3601 tctcggccgg gatccccggg ccatcgtcct tgacctcgag cacgacgaac cgcgcggtac
     3661 cgggatcgcc cggcgcgccg tgatcgcggg tccgcaccac cacgcgcccc gacgactgca
     3721 ccgcgtcgcg cgcgttgagc accaggttca tgatcacctg ctcgagctgc gtcgggtcgc
     3781 cgaccacggt gccgcggtcg ctggcctcga tctcgaacgt cagcgtgacg ccgggcagcg
     3841 agcgggtcaa gagctcgctc atcgaggtca ccaggtcgtt gacgctgacc ggcgccgcgc
     3901 ggttcttgcc gcggcgggcg aacccgagca gcgaccgggt gagcgcggcc gagcgctcgg
     3961 tgatgtcgtc gatcagcttg agcgcctcgc ggcgcttggg gtcgggctcg ctcatcgcga
     4021 gctcggccgc gatcatcttg atcccgaaga tcaggttgtt gaagtcatgg gcgatgccgc
     4081 cggcgagcgt gccgatcgcc tcgagccgct gggcccggcg cagccgctgc tcggtctcgg
     4141 cgcgcgcgcg ctcggcctcg acctcgcggc tgatgtccca gaacgcgacg atcacgtgcg
     4201 tgatcgggtc gccgaccggg cgggcgacgg cgcggacctc gacccgcttg ccgtcgcgcc
     4261 ggtggatcgt gaggtcgtcg gcgacgatca cgcggcgctc ggcgatcgcg cgggtgaacg
     4321 gcatcaggtg ctcgggatac ggcgcgccgt cgcgggtgtg gacgccgtgc ggcgcggcga
     4381 acgcgccgac ccggacgccg gcgatcggca tcgtgcccat gatctcggcg aacgtgtggt
     4441 tggcgtagac cagctcgcca cggggcgcgc gagcgaccca cagcccgatc gggaggtcct
     4501 ccccgagcga gagaatgatg tcttggtccg acggtaccgc cacgggcctg tcggaccaaa
     4561 cttatcactt gcggacgcgg gagagcagct ttgccatatc gtccgcgtgc tcttcctcct
     4621 tggccaagat ctcctccatc atccgccgcg tggtcgggtc gtcgctcccc agccagcgga
     4681 cgatctcgga gtaggtggcg atcgcgatcc gctccgcgac caggtcctcc ttgatcatct
     4741 cctccaggtc gtccccctcg ccgtattgcg cgtggctgcg ggtcgccagg ccctcggggt
     4801 cgaggttcgg cttgccaccg agctcggtga tccgcttggc gacccggtcg gcgtggtcct
     4861 gctcctcgcg cgcgtgctcg aggaactcgt cctccactcc ggacgagtgg atcccccggg
     4921 ccatgaaata gtggctgcgg tagcgcaagg tgcagaccgt ctcggtcgca agtacctcgt
     4981 tcagcacgcc gatcaccttg ccgcggtcgg cccggtacga cgcggtgacc gcgccgtcgt
     5041 ccatcttctc gagggcgcgg cggcggatgt tctcgatatc tgcgcggaac gtgtccatga
     5101 ccgcgcacat gtcgcaactt cggggccaac gtaagacctc gaaactcatc gtggatcgtg
     5161 cgcggcggcg ccacatgtgc cgcggcgaca gcgcgctgtg ccaccatgtg gcacgcagtg
     5221 ctgcgatggc cgggtaatct cgttcacttc ctgtggctcg tcctcgtcgt gacggcatgc
     5281 ggggtgatgt acggcgtgct gcgactcgcg atcttcctgt tcgtgtgggg cccccggcgc
     5341 accccatcgc tcgggacgct gcgcggcgtc atcctgcgct gggcgatgac cgcgctggga
     5401 gccacgttca tcaagctggg ccaggtcatg agcacgcggc ccgacctgtt cgagcccgag
     5461 atcatcgccc agctgcgccg gctccaggac cggctgccgc cgttcccgtt ccaccgggtc
     5521 cgcgcgacgg tcgaggccga gctcggccgg ccgctcgtcg agctgttcgc cgacttcgag
     5581 cccgcgccgg tcgccgcggc cagcgtggcc caggtccacc gcgcccacct gcgcgacggc
     5641 cgcgaggtcg cggtcaaggt gctgcgcccc ggcgtgcgcc ggcaggtcga gcgcgaccag
     5701 gcgatcctca tcggcttcgc gcgcctcatc gcgatccatc cgacctggcg gcgctcggat
     5761 ccggtcggcc acacg
//
LOCUS       DASVOR010000649         4029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_94356,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000649 DASVOR010000000
VERSION     DASVOR010000649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94356"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1381)
                     /locus_tag="VF516_12815"
     CDS             complement(218..1381)
                     /locus_tag="VF516_12815"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX8108605.1"
                     /translation="MAVSSFALASCAMEAPAEDQQSDEFDAPTKQVCGGGTFVCKSHV
                     RVDENNRIKPSATPAGLGPADLASAYKLNAAITSTATIAIIDAFHYANAESDLAMYRS
                     TFGLPPCTVASGCLTILNPTHAKAPPAGDDWNLEAALDLDMASAACPTCKLVLVEATD
                     DVGNDLFNEQNTAAARAGVVSISNSWGESSPGNDATLDAQFFTHTQAVNTFVSSGDSG
                     NTGTGGDYPSTSEFVFAVGGTHLVKSTTAARGWTETAWSGAGSTCSGILPEPSFQIGT
                     VPSNACTRRAASDISAVADPNTGVAVFNKNDGGFLVVGGTSASSPFVAGVFARYGISG
                     ATHNASFAYSHKADFFDVTSGNDGRCRTALCRAGTGWDGPTGLGTPNGAVLAP"
     gene            complement(1653..2432)
                     /locus_tag="VF516_12820"
     CDS             complement(1653..2432)
                     /locus_tag="VF516_12820"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8108606.1"
                     /translation="MAEYAAIYTTILVDDAGPIARLTLNRPDKRNPIGPATCGELVTV
                     LAGIRDKPEVRVVVLTGAGAVFSAGGDLSAMQAPAAGPRASLVDLLLAMHDLGKPIIA
                     MVNGPALAGGLGLMVACDLVIAADTATFGTTEIAVGLWPMMITAEIVRNVGRKVALEM
                     MLTGRKLDAREALACGLVTRVVPAAELEAETARLAGELAERSPAAIALGLRAFYRSQD
                     MALEPQLRHLEAELGRVLALEDAAEGIAAFLGKRKPVWKGR"
     gene            2528..3538
                     /locus_tag="VF516_12825"
     CDS             2528..3538
                     /locus_tag="VF516_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108607.1"
                     /translation="MVRGAPAALAAALVVGLGGPVCAPRPAAADQNDLVMSRLATRIT
                     DDTGRVLAVVGQNLEFRALASQLGVVLAPHLLTPADTLGFAGFQFDADVSQTTIDSIQ
                     PYWRVLAGTRDPSGTNHIAHGAGVLRTVGMFAHKGLWFPAPSFELGAGAVHLVGSTIW
                     AAQLYGKLAVHEGFHDLPIPSVAVRGGVSRMMNQRELDLTIASFDVTVSRHLGIGGTW
                     RLDPFAGYDLLIIVPRSQVIDATPDVDPLAPGNQMDAANDFVFRDQANILRQRLLLGA
                     KLQYEVLQLTLEAQLALAGSSVDSHPGTMACQPSSTTTSCSARDTAAAQTTVSLSAGF
                     DF"
     gene            3687..>4029
                     /locus_tag="VF516_12830"
     CDS             3687..>4029
                     /locus_tag="VF516_12830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8108608.1"
                     /translation="MGEPIRIAICDDHPVFRAGLVGLLRDEPDLDIAFEAGSARELRD
                     RIAGAPVDVLLLDLQLPDEHGLAVLPAITDRCRAVIVSAFDDPVQIRRAFEHGAVGFV
                     RKDAAACAILDA"
BASE COUNT          565 a         1474 c         1375 g          615 t
ORIGIN      
        1 gcccagtgca ccgagctggt ctggcagctg cgcggccagg ccgaggcccg ccaggtcgag
       61 ggcgcgcgga tcgcgctgca gcacaacctc gggctcggcg gcgcctgcgt ggtcacgatg
      121 taccgccgcg actgaacgcg aaaaacgaaa ggcgcccggc cggctggccg agcgcctcgt
      181 tcactgcgca ggccaggcga gatgctcgcc ggaccgctca gggagcgagc acggcgccgt
      241 tcggcgtgcc gaggccggtc gggccgtccc agccggtgcc ggcgcggcac agcgcggtgc
      301 ggcagcggcc gtcgttgccg ctggtgacgt cgaagaagtc cgccttgtgg ctgtacgcga
      361 agctcgcgtt gtgggtggcg ccgctgatgc cgtagcgcgc gaacacgccg gcgacgaacg
      421 ggctcgaggc gctggtgcca ccgaccacga ggaagccgcc gtcgttcttg ttgaacaccg
      481 cgacgccggt gttcggatcg gcgaccgccg agatgtcgga cgcggcgcgc cgggtgcacg
      541 cgttgctcgg caccgtgccg atctggaacg acggctcggg caggatgccg ctgcacgtgc
      601 tgcccgcgcc cgaccacgcg gtctcggtcc agccgcgcgc cgcggtggtc gacttcacca
      661 ggtgggtgcc gccgaccgcg aacacgaact ccgacgtgct cgggtagtcg cccccggtgc
      721 cggtgttgcc gctgtcaccg ctcgacacga acgtgttgac cgcctgggtg tgggtgaaga
      781 actgcgcgtc cagcgtggcg tcgttgcccg gcgacgactc gccccagctg ttgctgatcg
      841 agaccacgcc cgcccgcgcc gcggcggtgt tctgctcgtt gaacaggtcg ttgccgacgt
      901 cgtcggtggc ctcgaccagc acgagcttgc aggtcgggca cgcggcgctg gccatgtcga
      961 ggtcgagggc cgcctccagg ttccagtcgt cgcccgccgg cggggccttc gcgtgggtcg
     1021 ggttcaagat cgtcaagcag ccgctcgcca ccgtgcacgg cggcaggccg aacgtgctgc
     1081 ggtacatcgc gaggtcgctc tcggcgttgg cgtagtggaa cgcgtcgatg atcgcgatcg
     1141 tcgcggtcga cgtgatcgcc gcgttcagct tgtacgcgct ggcgaggtcg gccgggccga
     1201 ggccggccgg cgtggcggac ggcttgatcc ggttgttctc gtcgacccgg acgtgcgact
     1261 tgcagacaaa cgtgccacca ccgcatacct gcttggtcgg ggcgtcgaac tcgtcgctct
     1321 gctgatcctc ggccggagcc tccatcgcgc agctcgcgag cgcgaagctg gagacggcca
     1381 cggtgaaaaa tggcgctata acgtgcctgg gcgtgaatcg catggcgcca caatgcacga
     1441 ggctgtgcgc tcctgtgcac gaaaagtctt gtcagtttct gagacactgt cctggcggat
     1501 tctcagtact tcaaacaggg tctgatgaac ttgggttatt gaatacaaat tcaaaaatcc
     1561 gagggggcaa aacgctgcgg gctgtctcac ctctggagag aaccacctcc cttgactcct
     1621 ttatgaaaca attaaaggag atcgggccgc ggtcaccggc ccttccagac cggcttgcgc
     1681 ttgccgagga acgcggcgat gccctccgcg gcatcctcga gcgcgagcac ccggccgagc
     1741 tcggcctcga ggtggcgcag ctggggctcc agcgccatgt cctgcgagcg gtagaacgcg
     1801 cgcagcccga gcgcgatcgc cgccgggctg cgctccgcga gctcaccggc gagccgcgcg
     1861 gtctcggcct cgagctcggc cgccggcacg acccgggtga ccagtccgca cgccagcgcc
     1921 tcgcgggcgt cgagcttgcg gccggtcagc atcatctcca gcgcaacctt gcgccccacg
     1981 ttgcgcacga tctcggccgt gatcatcatc ggccacagcc cgaccgcgat ctcggtcgtg
     2041 ccgaacgtcg cggtgtcggc ggcgatcacc agatcgcacg cgaccatcag cccgagcccg
     2101 ccggcgagcg ccggcccgtt gaccatcgcg atgatcggct tgccgaggtc gtgcatcgcg
     2161 agcaacagat cgaccaggct cgcgcgcggc cccgccgccg gcgcctgcat cgccgacagg
     2221 tcgccgcccg ccgagaacac cgcgccggcc ccggtcagca ccacgacgcg gacctccggc
     2281 ttgtcccgga tacccgcgag cacggtaacc agctcgccgc aggtcgcggg cccgatcggg
     2341 ttgcgcttgt cggggcgatt gagcgtcagc cgtgcgatcg gcccggcgtc gtcgaccagg
     2401 atcgtggtgt agatcgcagc gtactcggcc atgcccgcgg tgtaccacgc ccgtgcgggc
     2461 cgccggccgc cgccgcgtgc tccgcgtcgc gttgacaccc ggtccgcccc gagcctaggc
     2521 tcccgccatg gttcgtggag cgcccgcggc gctcgcggca gcgctcgttg tcggcctcgg
     2581 cggccctgtg tgcgcgccac gccccgccgc cgccgatcag aacgacctcg tgatgtcgcg
     2641 gctcgcgacc cggatcaccg acgacaccgg ccgcgtgctc gccgtggtcg gccagaacct
     2701 cgagttccgc gcgctcgcct cgcagctcgg cgtggtgctc gcgccccacc tgctcactcc
     2761 cgccgacacc ctcgggttcg ccggcttcca gttcgacgcc gacgtgtcgc agaccacgat
     2821 cgacagcatc cagccgtact ggcgcgtgct cgccggcacg cgcgatccga gcggcacgaa
     2881 ccacatcgcg cacggcgccg gcgtgctgcg caccgtcggg atgttcgccc acaagggcct
     2941 gtggttcccg gccccgtcgt tcgagctcgg cgccggcgcg gtccacctcg tcggatcgac
     3001 gatctgggcg gcgcagctct acggcaagct cgccgtgcac gagggcttcc acgacctccc
     3061 gatccccagc gtcgccgtcc gcggcggcgt gtcccggatg atgaaccagc gcgagctcga
     3121 cctgaccatc gcgtcgttcg acgtcacggt gtccaggcac ctcgggatcg gcgggacctg
     3181 gcggctcgat ccgttcgccg gctacgacct gctgatcatc gtgccgcgct cgcaggtgat
     3241 cgacgccacc ccggacgtcg atccgctcgc cccgggcaac cagatggacg ccgccaacga
     3301 cttcgtgttc cgcgatcagg ccaacatcct ccgccagcgc ctcctgctcg gcgccaagct
     3361 gcagtacgag gtgctccagc tcaccctgga ggcccagctc gcgctcgccg ggtcgtcggt
     3421 cgacagccac cccggcacca tggcctgtca gcccagctcg accaccacca gctgcagcgc
     3481 ccgggacacc gcggcggccc agaccaccgt gtcgctctcg gccggcttcg acttctgagc
     3541 gcgccgggca cgacctcggc cacggccgcg acatgacctt gtggacctgc cgggcatgac
     3601 caaagtaacg atctgcacag ccggtggtcg accgcatcgc gcggcgctgg actcggcgtc
     3661 gatcctcggc aggtaatagc cgggcgatgg gcgaacctat ccggatcgcg atctgtgacg
     3721 atcacccggt gttccgcgcg ggcctggtcg ggctgttgcg cgacgagccc gatctcgaca
     3781 tcgcgttcga ggccggctcg gcccgcgagc tgcgcgatcg gatcgccggc gcgccggtcg
     3841 acgtcctgct gctcgatctg cagctgcccg acgagcacgg gctcgccgtc ctgcccgcga
     3901 tcaccgaccg ctgccgcgcc gtgatcgtct cggcgttcga cgacccggtc cagatccggc
     3961 gcgcgttcga gcacggcgcg gtcgggttcg tgcgcaagga cgccgcggcg tgcgcgatcc
     4021 tcgacgcgg
//
LOCUS       DASVOR010000650         4533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_239891,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000650 DASVOR010000000
VERSION     DASVOR010000650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4533
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239891"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2095
                     /locus_tag="VF516_12835"
     CDS             <1..2095
                     /locus_tag="VF516_12835"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX8108609.1"
                     /translation="GSVVSLSLSFDATVTSLFVPLALGLRQVLLPEGDGQLERLSSIL
                     WESGEALLFKLTPAHLEALSRLGGTRASGQGHVRVVGGEQLTESVVHGLRQRLPSARI
                     VNEYGPTEATVGCSVYEVGASHRYTEGLSGAIPIGRPIANTQLYVLDGQRAPLPVGVV
                     GELYIGGVGVARGYLNKPELTAERFVADPFSGDPEARLYRTGDLARWLPDGTLEYVGR
                     IDTQVKIRGYRIELGEIEARIAEHAAVRDAVVVVREAAAGGKHLVAYLRPSPQHAPVL
                     QERLIADVRAHLDAVLPGYMVPATFVCVDQWPLSPNGKLDRRMLPDPGKGDTGKPPHR
                     SPRTAMERYLCTVYAEALRCDPPGIDDDFFAIGGHSLSVLSVHARLRRTLLDIPLQAL
                     YDHRCVADLAAYLESRGASLGIPVGASHCVVPLNESSCTRKLFIVHPLSGVASPYTAL
                     AAALADVRRCYGVQAPDSFEDIALETIAERVAFYTDAIVREQPEGPYDISGFSSGGWL
                     AYEVAVELARRGAMVASMSILDVVPPLDKFKQNPSTYYGAIKGLHQQELKMDWSALDG
                     LDEAEGIERVLAEAKVQELIPAGLGDDNARRHLRYLRNWAYSLNTYETSTSSLNLDVY
                     VSRVSESPDKQALYDDQMHAIWSRFTDGEVRTIACEGDHGTMVDPPHLASLVEALRAR
                     LAASDARSVPVGGQR"
     gene            2128..3375
                     /locus_tag="VF516_12840"
     CDS             2128..3375
                     /locus_tag="VF516_12840"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8108610.1"
                     /translation="MNFLNEVAADYPEAISFASGRPAQQFFDVDAWWEAPARFQRHYA
                     EQMGIDAVEAGRLIAQYGRTAGLINPLIAEQLGNDEGVRCRPDQVVVTAGCQEAMALC
                     IQVLCSSPEDVLLVRDPTYIGATGAADLFGVEVVPLSSVGEDDLCDELREVAARLTAN
                     GRRARALYLIPEFDNPTGRVLTTRCRARLIELCAETRIAVLEDNPYGMFRFEGESVPT
                     MWTLDREGVVIYLATYSKTLCPAVRIGCAVVPERLLGDGDAGRELVATLSERKSFLTV
                     NTSQTNQAMLGGVLLEGGGSLRRRIVPAIAFYRRNRDVLVDSLERAFGADPRCRWNVP
                     EGGFFLTLQLPFRFLQEDLVACASRYGVIPMPMSYFSFSRANDRAVRLAFSNNDPDAI
                     SEGVNRLARYVAERKQEHVDEVA"
     gene            complement(3491..4513)
                     /locus_tag="VF516_12845"
     CDS             complement(3491..4513)
                     /locus_tag="VF516_12845"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8108611.1"
                     /translation="MGIILILATQCAPKLHRREGRPLSSASTLRNGRAVSSPSPEGAR
                     AVASGCRPIFVLGADRSGTSMISETIYRWGANAGDITSLGGANEGNPRGYWEYEPMEE
                     LLDKLFAAAKVSPWHPEFPGRVRALARDPALRAEALRLAAGMEEGPAWFWKEPFLCRC
                     LPFFMDIIPSPLFVVAIRNPYESALSYEKYILPSTLRGTIRLRSLYLLRWQQLVVSVL
                     EHAVNHPSVIYLPYVELVNDPADQCGRLCAFLDRECGVDGDSTQRVQRMTQAVDPALR
                     RNRGDRLFDTADEATPTQRALFRHLLERVRDPALPFSAADYPLPPGGREYLENAELML
                     SMLRKM"
BASE COUNT          721 a         1415 c         1608 g          789 t
ORIGIN      
        1 tggcagcgtg gtgagcctct cgctgtcgtt cgacgcgacg gtgacctcgc tgttcgttcc
       61 gctggcgctg gggctcaggc aggtgctgct gccggaaggc gatggccagc tggagcgcct
      121 gtcgtcgatc ctgtgggagt ccggggaggc gctgctgttc aagctgacgc ccgcgcacct
      181 ggaggcgctg tcgcggctcg gtgggacgcg cgcgtcgggg caggggcacg tgcgggtcgt
      241 gggaggcgag cagctgacgg agtcggtggt gcacggactc cggcagcggc tgccgtcggc
      301 gcggatcgtg aacgagtacg gtccgacgga ggcgacggtg ggttgcagcg tgtacgaggt
      361 gggggcttcg caccggtaca cagaaggtct gtcgggagcg atcccgatcg gtcgtccgat
      421 cgcgaacacg cagctgtacg tgctggatgg ccagcgtgcg ccgctgccgg tgggcgtggt
      481 gggcgagctg tacatcggcg gcgtgggcgt ggcgcggggt tatctcaaca agccggagct
      541 gaccgcggag cggttcgtcg cagacccgtt ctcgggcgat ccggaggcgc ggctgtaccg
      601 gaccggagat ctggcgcgct ggctgccgga cgggacgctg gagtacgtgg gtcggatcga
      661 cacgcaggtg aagatccggg gctaccggat cgagctgggc gagatcgagg cgcgcatcgc
      721 ggagcatgca gcggttcggg acgcggttgt ggtcgtccgg gaggccgctg cgggaggcaa
      781 gcacctggtg gcgtacctgc gtccatcccc gcagcacgcg cccgtcctgc aggagcggct
      841 catcgccgac gtgcgggcgc atctcgacgc cgtcttgccg gggtacatgg tgccggcgac
      901 cttcgtctgc gtcgatcaat ggccgctgtc gccgaacggc aagctcgatc gccgcatgct
      961 gccggacccg ggcaaaggcg acaccgggaa gccacctcat cgctcgccgc gtacggcgat
     1021 ggagcgctac ctgtgcacgg tctacgcaga ggcgttgcgc tgcgatcctc ctgggatcga
     1081 cgatgacttc ttcgccatcg ggggccactc gctgtcggtt ctctccgtcc atgccaggtt
     1141 gcgccgcacc ctcctggata tcccattgca ggctctgtat gaccatcgct gcgtcgccga
     1201 tctcgcggcc tatctggaat cgaggggcgc ctcgctcggg attccagtcg gcgcatcgca
     1261 ctgcgtcgtt ccgttgaacg aatcttcctg cacgaggaag ctcttcatcg tccatccact
     1321 gtcgggcgtg gcatcgccat acacggcact cgccgccgca ctggcggatg tgcgcaggtg
     1381 ttatggcgtg caggcaccgg atagcttcga ggacatcgcg ctggagacca tcgccgagcg
     1441 cgttgcgttc tataccgacg ccatcgtcag ggaacagccc gaaggcccct acgatatctc
     1501 cggcttctcg tccggcggct ggctggcgta cgaggtggcc gtcgagctcg cgcgccgcgg
     1561 ggcgatggtg gcgagcatga gcatcctcga tgtcgtcccg ccgctggaca agttcaagca
     1621 aaacccctcc acctactacg gtgcgatcaa gggccttcac cagcaggagt tgaagatgga
     1681 ctggtccgcg ctcgacgggc tcgacgaggc cgagggaatc gagcgcgtcc tcgctgaggc
     1741 caaggtacag gagctgatcc cggcagggct cggagacgac aatgcaaggc gtcatctccg
     1801 ctatcttcgc aactgggcgt acagcttgaa tacctacgag acttcgacgt cgtcgttgaa
     1861 cctggatgtc tatgtatcca gggtcagcga atcgcctgat aagcaggcgc tgtacgacga
     1921 ccagatgcac gccatctgga gtcgcttcac cgacggggag gtgcgcacga tcgcctgtga
     1981 aggcgaccat gggacgatgg tcgacccgcc gcacctcgct tccctggtcg aggcgctgcg
     2041 ggcgcgcctg gcggcgagtg acgctcgaag cgtgccggtg ggtgggcagc gatgagcgcg
     2101 cggtcgcatc cctcccgact caaggtcatg aacttcctca acgaggtggc tgccgactac
     2161 cccgaggcga tctcgttcgc gtcgggccgt ccggcccagc agttcttcga cgtggatgcc
     2221 tggtgggagg cgcccgcgcg cttccagcga cactacgccg aacagatggg gatcgacgcc
     2281 gtcgaggcgg ggcgcctgat cgcgcagtac gggcgtaccg cgggcttgat caacccgctg
     2341 atcgcggagc agctgggcaa tgacgagggc gtgcgttgcc gaccggacca ggtggtcgtc
     2401 accgccggtt gccaggaggc catggcgctg tgcatccagg tgctgtgctc ctcgcccgag
     2461 gatgtcctgc tggtgcgcga tcccacctat atcggcgcga ccggcgccgc cgacctgttc
     2521 ggcgtggagg tggtgccgtt gagttcggtg ggcgaggacg acctgtgcga cgagctgcgg
     2581 gaggtcgcgg cgcgcctgac ggcgaacggc cgccgtgccc gggccctcta cttgatcccg
     2641 gagttcgaca accccaccgg gcgggtgttg acgacccgct gcagggctcg cctgatcgag
     2701 ctctgcgcag aaaccaggat cgcggtgctg gaggacaatc cctatggcat gttccgcttc
     2761 gagggggagt cggtacccac gatgtggaca ctggaccgcg agggggtggt gatttacctc
     2821 gcgacctact ccaagaccct ctgcccggct gtccgcatcg gctgtgcggt ggtgccggag
     2881 cgcctgctgg gcgacggaga cgccggtcgt gaactggtgg cgacgctgtc cgagcgcaag
     2941 agctttctca ccgtcaacac cagtcagacc aaccaggcga tgctcggtgg cgtgctgctg
     3001 gagggtggcg gatcgttgcg tcggcgcatc gtgccggcga tcgcgttcta caggcgcaac
     3061 cgggatgtcc tggttgactc gctcgagcgg gcctttggcg cggacccgcg gtgccgatgg
     3121 aacgtgccgg agggggggtt cttcctcacc ctgcagctac ccttccggtt cctgcaggag
     3181 gatctggtcg cgtgcgccag ccgctatggc gtgatcccga tgccgatgtc ctatttctcc
     3241 ttcagcaggg ccaacgaccg cgccgtccgc ctggcgttca gcaataacga ccctgacgcg
     3301 atcagcgagg gagtgaatcg cctggcccgt tatgttgccg agcgcaagca ggagcatgtc
     3361 gatgaagttg cttgaccgtc acacgatcga gggatccccg tcaatgagca cgtttaccgg
     3421 ctgctcgcca gctacctgtg atcggctccg tacccagctg cggcgcgagg gcacggagcc
     3481 atgacgagga tcacatctta cgcagcatcg aaagcatcag ttcggcgttc tccaggtatt
     3541 ccctgcctcc ggggggcagg gggtagtcgg ccgcggagaa cggcagcgcg gggtcccgta
     3601 cccgttccag caggtggcgg aacagcgccc gctgggtcgg cgtggcctcg tcggcggtat
     3661 cgaacagacg gtcgccgcga ttgcggcgta gtgcgggatc cacagcctgc gtcatccgct
     3721 gcacgcgctg ggtgctgtcg ccgtccacgc cgcattctcg gtcgaggaag gcgcaaaggc
     3781 ggccacactg gtcggccggg tcgttgacca actccacgta gggtaggtag atgacactgg
     3841 gatgattgac ggcgtgctcc aggaccgaga ccacgagctg ctgccagcgc agcaggtaca
     3901 ggctgcgcag gcggatggtg ccgcgcagcg tgctggggag gatgtacttc tcgtaggaga
     3961 gcgcggactc gtacgggttg cggatagcca ccacgaacag gggagatggt ataatgtcca
     4021 tgaagaacgg caagcagcgg cagaggaagg gttccttcca gaaccacgcc ggcccttcct
     4081 ccatgcccgc ggccagccgc agcgcctccg cgcgcaaggc cgggtcgcga gccagcgcgc
     4141 ggactcgccc ggggaactcc ggatgccatg gcgaaacttt cgctgctgcg aacaacttgt
     4201 cgagcagttc ctccatcggc tcgtattccc aatagcctcg aggattaccc tcgttggcgc
     4261 cacccagcga tgtgatgtcg ccggcgttcg ccccccagcg atagatggtt tcggagatca
     4321 tcgaggtgcc gctacggtcg gcaccaagca cgaagatcgg cctgcagcca ctagccacgg
     4381 ccctggcgcc ttccggagag gggcttgaca ccgcacggcc gttccggagc gtgctggcac
     4441 tcgacagggg gcgtccttcg cggcgatgga gcttgggggc gcattgcgtg gcgagtatga
     4501 ggatgatgcc catgcaccgt caagcggcgc gtg
//
LOCUS       DASVOR010000651         2025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_444375,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000651 DASVOR010000000
VERSION     DASVOR010000651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444375"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1280
                     /locus_tag="VF516_12850"
     CDS             114..1280
                     /locus_tag="VF516_12850"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8108612.1"
                     /translation="MAYAALDYYAIDELFSHEERLVRDTVRELVTTRVMPTIGKHWAE
                     GTFPHELIAELGKLGLLGSSLTGYGCAGITPTAYGLVCQELERGDSGIRSFCSVQSSL
                     VMYPIWAFGSEAQKQRYLPEMAAGRVIGCFGLTEPDFGSNPAGMLTTAVRTGKGYQIT
                     GTKRWITNGSIAQVALVWAKLDGRIHGFLVPTSTPGFSARNIHGKWSLRASVTSELVL
                     EDCEVGEDALLPGVSGMRGPLSCLSQARFGICYGAVGAAMACYDEALRYAQDRVQFKK
                     PIAGYQLVQRKLVDMVSEITKAQLMCWRLGRLKEAGTITGGQISLAKRNCVSIARDIA
                     RDARDILGANGVTDEYQCGRHMLNLESVYTYEGTHDIHTLVVGQDITGLAAFDS"
     gene            1277..>2025
                     /locus_tag="VF516_12855"
     CDS             1277..>2025
                     /locus_tag="VF516_12855"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEX8108613.1"
                     /translation="MTHPFRAPAPDAPDVLICAGLDPSGGAGLIADVRVVSALGCRPV
                     GVVTALTVQNTTAVVDTEAMNAERVREQLEFLLSDVEVRAVKIGMIGSSAIAQGVGRA
                     LALTAAPVVWDPIVHPSRGDVRFVDSLFGHAVAALAPHVTVLTPNAGELAFMTGTAIA
                     SLDDARVVGRALAERLRTAVLVKGGHLGGDESRAESSAESSHESVDLLCEPGGIVELR
                     GRRIAGGEHVHGTGCALSSALAAHLARGLALV"
BASE COUNT          287 a          726 c          721 g          291 t
ORIGIN      
        1 cagaccgtcc cggagcagat cgttgccgta cgcaccaccg gcgaagtata ccgccccggc
       61 acatcccttc acgtggagcc ctcgcatccc ccggggggcg cggagaccaa gccatggcgt
      121 acgccgcact ggattactac gcgatcgacg agctgttcag ccacgaggag cgcctggtgc
      181 gcgacaccgt ccgcgagctg gtgaccaccc gggtgatgcc gacgatcggc aagcactggg
      241 ccgagggcac gttcccccac gagctgatcg ccgagctcgg caagctcggc ctgctcggca
      301 gctcgctgac cggctacggc tgcgccggga tcacgccgac cgcgtacggc ctggtctgcc
      361 aggagctcga gcgcggcgac tcgggcatcc ggtcgttctg ctcggtgcag agctcgctgg
      421 tgatgtaccc gatctgggcg ttcggctccg aggcccagaa gcagcgctac ctgcccgaga
      481 tggccgccgg ccgcgtgatc ggctgcttcg gcctgaccga gcccgacttc gggtccaacc
      541 ccgccgggat gctgaccacc gcggtgcgca ccggcaaggg ctaccagatc accggcacca
      601 agcgctggat caccaacggc tcgatcgccc aggtcgcgct ggtgtgggcc aagctcgatg
      661 gccggatcca tggcttcctg gtcccgacca gcactcccgg gttctcggcg cgcaacatcc
      721 acggcaagtg gtcgctgcgc gccagcgtga ccagcgagct ggtgctcgag gactgcgagg
      781 tcggcgagga cgcgctgctg cccggggtca gcggcatgcg cgggccgctg tcgtgcctgt
      841 cgcaggcgcg gttcgggatc tgctacggcg cggtcggcgc ggcgatggcg tgctacgacg
      901 aggcgctgcg ctacgcgcag gaccgcgtgc agttcaagaa gccgatcgcc ggctaccagc
      961 tggtccagcg caagctcgtc gacatggtgt ccgagatcac caaggcgcag ctgatgtgct
     1021 ggcggctcgg ccggctcaag gaggccggca cgatcaccgg cggccagatc tcgctcgcca
     1081 agcgcaactg cgtctcgatc gcgcgcgaca tcgcccgcga cgcgcgcgac atcctcggcg
     1141 ccaacggcgt caccgacgag taccagtgcg gccgccacat gctgaacctc gagtcggtct
     1201 acacctacga gggcacgcac gacatccaca cgctggtcgt cggccaggac atcaccgggc
     1261 tggcggcgtt cgactcgtga cccatccgtt ccgtgcgccc gcgcccgacg cgcccgatgt
     1321 cctgatctgc gccggcctcg atccgtccgg cggcgccggg ctgatcgccg acgtccgtgt
     1381 ggtcagcgcg ctcggctgcc gcccggtcgg cgtggtgacc gcgctgaccg tgcagaacac
     1441 gaccgcggtg gtagacaccg aggcgatgaa tgccgagcgc gtgcgcgagc agctcgagtt
     1501 cctgctgtcc gacgtcgagg tccgggcggt gaagatcggg atgatcggct cctccgcgat
     1561 cgcccagggc gtcggccgtg cgctcgcgct caccgcggcg ccggtggtgt gggatccgat
     1621 cgtgcatccg tcgcgcggcg acgtgcggtt cgtcgacagc ctgttcggcc acgcggtcgc
     1681 ggcgctcgcg ccccacgtca ccgtgctgac ccccaacgcc ggcgagctcg cgttcatgac
     1741 cgggaccgcg atcgccagcc tcgacgacgc gcgcgtcgtc ggccgggcgc tggccgagcg
     1801 gctgcgcacg gcggtgctgg tcaagggcgg ccacctgggc ggcgacgaga gccgtgccga
     1861 gagcagcgcc gagagcagcc atgaatcggt cgacctcttg tgcgagcctg gcgggatcgt
     1921 cgagctgcgc ggccggcgga tcgcgggcgg cgagcacgtc cacggcaccg ggtgcgcgct
     1981 gtcgtcggcg ctcgccgcgc acctcgcgcg cgggctggcg ctggt
//
LOCUS       DASVOR010000652        13563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_94382,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000652 DASVOR010000000
VERSION     DASVOR010000652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13563
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94382"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /gene="cysC"
                     /locus_tag="VF516_12860"
     CDS             complement(<1..521)
                     /gene="cysC"
                     /locus_tag="VF516_12860"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX8108614.1"
                     /translation="MIGGIVWMTGLSGAGKSTLATALAARLAPVRPVELLDGDDVRTF
                     LSAGLGFSRADRDTNVNRIAFVARLLARHGVLVLVAAISPYAATRARLRELSDAAGHP
                     FVEVFVHAPLETVIERDVKGLYRRAQAGEIPSFTGISDPYEPPAAPDVEVRTDREDRA
                     SCEVRILQALADR"
     gene            618..1676
                     /locus_tag="VF516_12865"
     CDS             618..1676
                     /locus_tag="VF516_12865"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8108615.1"
                     /translation="MRPGGPQARYAFVDALRGVAAIGVVLYHVYSKNLLPMSGYRLPE
                     PLHAIGNNGNLGVYIFFVISGFVITQSIRSSHITPRFVGWFALRRSLRLDPPYWATIA
                     AMIALSYISGRVQRDHAPLPLPTWDAVVAHLFYLQGFLGYPQIVGVFWTLCYELQFYL
                     ALVVLTGVWQWLRPALRTPWVVFGPLWVASLWCITRSPDPTDEALAVYGWPYFFLGVA
                     VNWVHNGDEPPHVLAIVAAGTAALLPFAPLKAGIALVTAGAIYAVSASDKLTSLTLGR
                     PLQYLGRISYSLYLVHMLIGTPLVRFGLRHLGPVHFTTAVALTCLGVAASVAAAHVMY
                     VVIERPAVRWSRRLREAR"
     gene            complement(1692..2810)
                     /locus_tag="VF516_12870"
     CDS             complement(1692..2810)
                     /locus_tag="VF516_12870"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8108616.1"
                     /translation="MTQSAPASTVGAAPRGERAEIVAMTSMRGVAAALVVAYHLKGRG
                     YDSWVRGGYLWVDFFFVLSGFIMAYVYGQMFEREVSVSSYRAFLGKRIARIYPLHLFM
                     LCAYIACGAAGVSACSGGVHGFFTNLFLIQAWGTEHDLTWNRVAWSISAELGFYLVFP
                     FIARAIATTPRSLHAIAPVALFGGLVLLVHHTGTLGVTYDFALIRCIFEATIGMLLYQ
                     VMRDPAPGVKWLATRDWVRWPLLFAPLVLMQFHAHEVAIVAVFALMILPLAFADRSFD
                     RFLRWAPVRHTGVVSYSLYMCHYLVLIFVEGALKRLHLAPSHDDLGLAQLIAVYIGFI
                     TLCVAVATVLYKLVEVPGRRAMNRYFAMRRARLAQAQG"
     gene            complement(2931..3551)
                     /locus_tag="VF516_12875"
     CDS             complement(2931..3551)
                     /locus_tag="VF516_12875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108617.1"
                     /translation="MNTKALITTLAVLGSTSSLAMAHPTHDSAPVSVSAYASWNTGGY
                     ATEYQAPYATEYQAPIVRDHRYAPPPPPVRVRPVVQHRGGDWGRRDGWGYDGQPRETI
                     LADGLTYSAGEFRKDIDLQGRGRFNAVTLHEDDGCNFIAQVVVEFMGGAVQSIPVNRT
                     LQPNQSMTFDLNGTNRAINRIFVYRDYSGQRNTRVPSGGDFTVAAL"
     gene            complement(3808..4863)
                     /locus_tag="VF516_12880"
     CDS             complement(3808..4863)
                     /locus_tag="VF516_12880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX8108618.1"
                     /translation="MKVIIPCGGRSSRYPNQPPKWMLPAHDGRPMLALAVSGVRVAKD
                     DLIVTVLREHEDRYRVRQGLEAAFGQPIRVVVLPEPTRSQPETVARTLEAVDLREPFL
                     VKDSDNTFIVDDVVQETNYVCVDSLNNYDQINPRNKSYLQVDHRDLVTNIREKVVISD
                     LFSVGGYGFLHPDQFLEHYRRLSHHSAEWQRELYISDIIGAMILDGVPFRACRIKGYQ
                     DWGTIAEWRRALLSRKSYFVLLDGFLFERGSEHFHPGFADVKPNAAAVDAVTELAGRG
                     HSFIYLSIRPAALADLTERQLAAAGLPAARVVYGCPISPWVMLTAPDPTLPFQTSHAL
                     ELGPDDPNLAGKLAFET"
     gene            complement(4860..5777)
                     /locus_tag="VF516_12885"
     CDS             complement(4860..5777)
                     /locus_tag="VF516_12885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX8108619.1"
                     /translation="MTSEPLLADPLLSDAADARPLSGLSGARVVLMTRDGRRWFVRKA
                     ARDPAGSARLRRQLEKQLAFGQGLGGAVRVPPVLGHGEVDGRFYFDMEFVRGLDGISY
                     LRRASYGEVIAVADRLCGYLDEIATRAPTGSTGPSLFDALYTKLCDVHRQTDEPGGET
                     LARLFLALDRLRALGAGLGPTMCHGDLTLENLVVDEHGAFWLLDFLDAPFEHWWQDVA
                     KLHQDLDGGWYLLSQPAIARCVLDYLSRRLLAAASRRAPMYPQVHAVLLACTFVRILP
                     YARTTQEWQFVKARVEHYARASSEEMKRS"
     gene            complement(5774..6388)
                     /locus_tag="VF516_12890"
     CDS             complement(5774..6388)
                     /locus_tag="VF516_12890"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HEX8108620.1"
                     /translation="MAARGLIFDLDGTLVDTMPIHYRAYQQTFAEAGIELTPEDFFGN
                     IGGKASETIPRFLRGRSCARSVDELHARKQELARQLLRTEDLPVLAAAELVTAFRGAL
                     PIALASSGSRSGIEVILDRLGWRGHFDAIVTGQDATRGKPAPDIFLLAAERLAVPPGR
                     CVVFEDTDDGVAAGRAAGMTVFDVRRAQAPSSAVLDLLAPARSR"
     gene            complement(6399..7655)
                     /locus_tag="VF516_12895"
     CDS             complement(6399..7655)
                     /locus_tag="VF516_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108621.1"
                     /translation="MLVGVVFATISHLRLAHVANTVHFENIVFGADNLDNFKGIMSGR
                     YGVVGSRKHALAQVVFAVLTAPQKWLGVSARASAMNTLALVVGLFGASFLVLCIRLGA
                     SLAFALASCLFVFATNAFAFQTALFETYILTSLVSTLAVLLFLELADHVTARPVWCAI
                     FAGTAAGIAGLANTSAAAIVLVYSSIVWSRLDRRPRCSRALLSIAMPCAIALTCAVLP
                     SVARDFLHHDAPLQWAVQYTSQWGNLGNLSNASLTSDHVVTFIVFSWVSAGIEPICRY
                     IDSDVVHLAHAPWAMLGLVGGLALVAYAVRRIVREPGAWPLAVGLAIVYGVFFAFYWY
                     FNPWEGMLYSCQWMLMVVCLVVLGLKTARGAAIAILVVAVAMLVSNEPLTWPTRGTFE
                     QACPDDHLPPWEQGPDPATPRTPEVV"
     gene            8325..8675
                     /locus_tag="VF516_12900"
     CDS             8325..8675
                     /locus_tag="VF516_12900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108622.1"
                     /translation="MLFCVVSAASATIGGTLMRKAALAGPILPDQWLPPHVTRGMLLL
                     GGALLAYGMSFIGYFLAVRLASLRLAYPVMVGFTCIGLYSVSVLYFGEPRRLLDVAGI
                     LLVLAGVAILARAA"
     gene            complement(8827..9963)
                     /locus_tag="VF516_12905"
     CDS             complement(8827..9963)
                     /locus_tag="VF516_12905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8108623.1"
                     /translation="MHPAANPDGAVALSVMIPNYNYARYIGETIESVLAQDLGRDLGR
                     DRSQLEIVVCDNASTDDSVSVVRGFRDPRIRLAVNPCNVGFAPNLERVAAMARGRRML
                     LLSSDDKMTPGALAAYARLETALGPRADTAVWGSSTAVIDSKGQRTGVQFDPDPKCWR
                     DSRDEPELTRAVGYPVRSMPAAALLKRSLELLRSPLPFLTTCYPKALHDAVGSYAGGR
                     LMNPDKWFLWKVLSASETAYKIDHPLFEYRVHDSGQGAQEQRSGALKHLADEYIATFN
                     LPEEVLRKAGVTRDVLAAAFIEQDIALRGLLALSQGHRRTARRGIDFGRAAYPELLRA
                     NRKVWALRALLMLGPVGTRVAAALRERVERRWKEQESRPSHDVR"
     gene            10281..12260
                     /locus_tag="VF516_12910"
     CDS             10281..12260
                     /locus_tag="VF516_12910"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HEX8108624.1"
                     /translation="MPRFLGRSIRLVFDLGVMSLAYWLAWLFRFEFQIPAHVLKVAML
                     TWPCVIGLQYGTLVAFGVERIAWRYINMRDAMRVLTATVISATVLVGFRIAHLLPVTT
                     SIIPLGVLAMDFVLGLVGLIGARAAWRVHHELRARRRHPQNGDRHRVFVIGAGEAGVM
                     VAREIVQRPDLHLEPVGFLDDDPFKVGKIVGGFQVLGMTHEVGVLAARLRVTRALITI
                     ANASGADLRRITELCRDAGLDTRIIPGLHEIVGDKVSVSRAREVAIEDLLGREPVKLD
                     EAVVSACLRGRVVLVTGAGGSIGSELCRQICRFAPRRLVLVERFENALFEIHRELTAA
                     FPGVRIDPRIADVCDMDRLAQVFEHTRPEIVFHAAAHKHVAMMEWNPGEAVKNNVGGT
                     RVVATLSDRYQVERFVLVSTDKAVNPSSVMGATKRVAEIYLQAFSQRSATRFVTVRFG
                     NVLGSAGSVVPIFRQQIAAGGPVLVTHPEMRRYFMTIPEASQLVMQAGAMGEGGEIFI
                     LDMGEPVKIVDLARDLITLSGLRPGDDIEIVFTGMRPGEKLFEELSTAAEHAEKTRHP
                     KVLIGKIKPHAWTEVIDGVNHLLDVAGRAEAGMGAGTDAGTETDLVRAALIALVPECR
                     FVRPHVTGRKSSPRGERALTGPHELALAAGGAIRN"
     gene            12271..13428
                     /locus_tag="VF516_12915"
     CDS             12271..13428
                     /locus_tag="VF516_12915"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEX8108625.1"
                     /translation="MRDHFLPFALPDMDEAEVAAAAEVIRSGWLTTGGKVRAFEAAFA
                     ERVGARHAIAVNSCTAALHLALEAIGVKPGDRVVTTPYTFATSGEVIRYLGAHPVFVD
                     VRPDTLNIDPDRVAAALARDRAAAILPVHIAGEPCDLDALRAVAGDIPIIEDAAHALP
                     TRLGDRMIGALSRVTCFSFYATKTLSTGEGGMITTDDDALADRCRIMSLHGISRDAWK
                     RYTAEGSWRYEILAPGFKYNLTDLAAALGLVQLGKLDAMWRRRAELAARYDRAFAGCP
                     ELELPARSAGNQHAWHLYMLRVDNDRLAGGRDALVTELRRRNIGASVHFIPLHLHPYY
                     RDTYGFQPSDFPIATREFEREVSLPIYSRMTDRDADDVIAAVHDSVAALRR"
BASE COUNT         2168 a         4679 c         4654 g         2062 t
ORIGIN      
        1 tggcggtcgg ccagcgcttg caggatgcgc acctcgcagc tcgcgcggtc ctcccggtcg
       61 gtgcgcacct cgacgtccgg cgcggccggc ggctcgtacg gatcggagat cccggtgaag
      121 ctcgggatct cgccggcctg ggcccggcgg tacagcccct tgacgtcgcg ctcgatcacg
      181 gtctcgagcg gggcgtggac gaacacctcg acgaacggat ggccggcggc gtcgctgagc
      241 tcgcgcagcc gcgcgcgggt cgcggcgtac ggcgagatcg cggcgaccag caccagcacg
      301 ccgtgcctgg cgagcagccg cgcgacgaac gcgatgcggt tcacgttggt gtcgcggtcg
      361 gcgcgcgaga accccaggcc cgcggacagg aacgtgcgta cgtcgtcgcc gtcgagcaac
      421 tcgaccggcc ggaccggcgc cagccgcgcc gcgagcgcgg tcgccagcgt cgacttgcca
      481 gcccccgaga gcccggtcat ccagacgatg ccgccgatca cgtgcgcttt atagcgcagg
      541 tcgtcggatc gacgcacccg gcggccacgg ttgcgctacg ctgagccgtc gcccggcggt
      601 ccgcccggcg ctcccgcatg cgtcccggcg gcccccaggc tcgatatgcg ttcgtcgacg
      661 cgctgcgcgg cgtggctgcg atcggggtgg tcctgtacca cgtgtacagc aagaacctgc
      721 tgccgatgtc gggttaccgg ctccccgagc cgttgcacgc gatcggcaac aacggcaacc
      781 tcggcgtcta catcttcttc gtgatcagtg gcttcgtgat cacccagagc atccggagct
      841 cgcacatcac gccgaggttc gtcggctggt tcgcgctgcg gcgctcgctg cgcctcgatc
      901 cgccgtactg ggcgacgatc gcggcgatga tcgccctgtc gtacatctcg ggccgcgtcc
      961 agcgcgatca cgcgccgctg ccgctgccga cctgggacgc cgtcgtcgcc cacctgttct
     1021 acctccaggg cttcctcggc tacccgcaga tcgtcggggt gttctggacc ctgtgctacg
     1081 agctccagtt ctacctggcg ctggtggtgc tgaccggcgt ctggcagtgg ctgcggcccg
     1141 cgctgcgcac accgtgggtc gtgttcggcc cgctgtgggt cgcctcgctg tggtgcatca
     1201 cccgctcgcc cgatccgacc gacgaggcgc tggccgtgta cggctggccg tacttcttcc
     1261 tcggcgtcgc ggtcaactgg gtccacaacg gcgacgagcc gccgcacgtg ctcgcgatcg
     1321 tcgccgccgg caccgccgcg ctgctgccgt tcgcgccgct caaggccggc atcgcgctcg
     1381 tcaccgccgg cgcgatctac gcggtgtcgg cgtccgacaa gctgacgtcg ctcacgctgg
     1441 ggcgccccct ccagtacctc ggccggatct cgtacagcct ctacctcgta cacatgttga
     1501 tcggcacgcc gctcgtgcgg ttcgggctcc gccacctcgg cccggtccac ttcaccacgg
     1561 ccgtcgcgct gacctgcctc ggggtcgcag cgagcgtggc ggccgcgcac gtgatgtacg
     1621 tcgtgatcga gcggccggcc gtacgctgga gccgtcgctt gcgcgaagcc cggtagcgga
     1681 ctccttcgcg atcatccctg cgcttgcgcg agccgcgccc ggcgcatcgc gaagtaccgg
     1741 ttcatcgcgc gccgccccgg gacctcgacg agcttgtaca gcacggtcgc gacggcgacg
     1801 cacagcgtga tgaagccgat gtagaccgcg atcagctggg cgagccccag gtcatcgtgg
     1861 ctcggagcga ggtgcaagcg cttgagggcc ccctcgacga agatcagcac caggtaatgg
     1921 cacatgtaca gcgagtacga gaccacgccg gtgtggcgga ccggcgccca gcgcaggaat
     1981 cgatcgaacg atcgatcggc gaacgcgagc ggcaggatca tcagggcgaa gaccgcgacg
     2041 atcgcgacct cgtgcgcgtg gaactgcatc agcaccagcg gcgcgaacag aagcggccac
     2101 cgcacccagt cgcgggtcgc aagccacttc acgcctggcg ccgggtcgcg catgacctgg
     2161 tacagaagca tgccgatcgt cgcctcgaag atgcagcgga tcagcgcgaa gtcgtaggtc
     2221 acgccgagcg tgccggtgtg atggacgagc agcacgagcc caccgaacag cgcgaccggc
     2281 gcgatcgcgt gcagcgaccg cggcgttgtc gcgatcgcgc gcgcgatgaa cggaaagacc
     2341 aggtagaagc cgagctcggc gctgatcgac cacgccacgc gattccacgt caggtcgtgc
     2401 tcggtgcccc acgcctggat aaggaacagg ttggtgaaga agccgtgcac gccaccggag
     2461 cacgcgctga ccccggcggc cccgcaggcg atatatgcgc acagcatgaa caggtgcagc
     2521 gggtagatgc gcgcgatccg cttgccgagg aacgcacggt agctcgaaac gctgacctcg
     2581 cgctcgaaca tctggccgta gacataggcc atgatgaagc cgctgaggac gaagaagaag
     2641 tcgacccaga ggtagccgcc gcggacccac gagtcgtagc ctctgccctt gaggtggtac
     2701 gcgacgacga gcgcggcggc gacaccgcgc atcgaggtca tcgccacgat ctcggcgcgc
     2761 tcgccgcggg gcgcagcacc gacggtggag gcgggagcgg actgggtcac gggttcgcac
     2821 atcatgccag acccgcacgc accgacccgg aaaatcacga ggatgtcgag cgagaccagg
     2881 cgatgcgaag cagatcctgt cctcgacacc gccctgaacg cgggtctcga tcaaagcgcc
     2941 gcgacggtga agtcgccgcc gctcgggacc ctggtgttcc tctggccgct gtagtcgcgg
     3001 tacacgaaga tccggttgat cgcgcggttg gtgccgttga ggtcgaacgt catcgactgg
     3061 ttgggctgca gagtccggtt gaccgggatc gactgcacag cgccgcccat gaactcgacc
     3121 acgacctgcg cgatgaagtt gcagccgtcg tcctcgtgga gggtcacagc gttgaaccgg
     3181 ccgcgaccct ggaggtcgat gtccttgcgg aactcgccgg cgctgtaggt caggccgtcc
     3241 gccaggatgg tctcccgggg ctgcccgtcg tagccccagc catcgcggcg accccagtcg
     3301 ccgccgcggt gctgcacgac gggacggacg cggacaggcg gcgggggagg cgcgtagcga
     3361 tggtcgcgga cgatcggggc ctggtattcg gtcgcgtacg gggcctggta ttcggtcgcg
     3421 tacccgccgg tgttccacga ggcgtacgca ctgaccgaga ccggcgccga gtcatgggtc
     3481 gggtgcgcca tcgcgagcga cgacgtggac ccgaggacag cgagcgtggt gatgagtgcc
     3541 ttggtgttca tgacgatttc tcctgggtcg gcagcgcttg cgcccgccga tgaccggaga
     3601 tcgtgcagcc gccaggccac ccctaagcca ttgaaatgac gtagtattac atctcgcact
     3661 cctgcgaggt ccgcagccgg actgcgggat ccgcagtcgt cccgcgaccc gctcccggac
     3721 gacctcgccg gcgcgccggg ccgacccggt atcccacagc gccggccaca gcgctggcca
     3781 cagcgccggc cacagcgcag gcctcggtca ggtctcgaac gccagcttgc cggcgaggtt
     3841 cggatcgtcc ggcccgagct cgagcgcgtg gctggtctgg aacggaagcg tgggatccgg
     3901 cgccgtgagc atcacccagg gcgagatcgg acagccgtag accacgcgcg ctgcggggag
     3961 gcccgccgcc gcgagctgcc gctccgtcag gtcggcgagc gcggccggac ggatgctcag
     4021 gtagatgaag ctgtgcccgc gtccggccag ctcggtgacg gcgtccaccg cggcggcgtt
     4081 cggcttgacg tcggcgaacc cgggatggaa gtgctcgctg ccgcgctcga acaggaagcc
     4141 gtcgagcagg acgaaatagg actttcgcga cagcagcgca cgccgccact cggcgatcgt
     4201 cccccagtcc tggtagccct tgatccggca cgcccggaac gggacgccat cgagaatcat
     4261 cgcaccgatg atgtcggaga tgtacagctc gcgctgccac tccgccgaat ggtgcgacag
     4321 gcgccggtag tgctcgagga actgatcggg gtgcaggaac ccataaccgc cgacgctgaa
     4381 caggtccgag atcacgacct tctcgcggat gttggtcacc agatcgcgat ggtcgacctg
     4441 caggtagctc ttgttgcgcg ggttgatctg atcgtagttg ttgagtgaat cgacacagac
     4501 gtagttggtc tcctgcacga cgtcgtcgac aatgaacgtg ttgtcggagt ccttgaccag
     4561 gaacggctcg cgcaggtcga ccgcctcgag cgttctcgcc acggtctcgg gctgactgcg
     4621 cgtcggctcc ggcaggacga ccacccggat cggctggccg aaggcggcct cgaggccctg
     4681 gcgaacccgg tacctgtcct cgtgctcgcg caacaccgtc acgatcaggt cgtccttggc
     4741 gacccggaca ccggacaccg cgagcgccag catcggccgg ccgtcgtgcg caggcagcat
     4801 ccacttgggc ggttggttcg gatagcggct ggaccggccg ccgcacggaa tgatgacctt
     4861 catgatcgct tcatttcctc cgaggaagcc cgtgcatagt gctcgacgcg ggccttcacg
     4921 aactgccatt cctgggtcgt gcgtgcatac ggcaggatcc ggacgaaggt gcacgcgagc
     4981 agcacggcat ggacctgtgg gtacatcggc gcgcgccggg acgcggccgc gagcaggcgg
     5041 cggctgaggt agtcgagcac gcagcgcgcg atcgccggct gcgacagcag gtaccagccg
     5101 ccgtcgagat cctggtgcag cttcgcgacg tcctgccacc agtgctcgaa cggtgcatcg
     5161 aggaagtcga gcagccagaa cgcgccgtgc tcgtcgacga cgaggttctc cagcgtcaga
     5221 tcaccgtggc acatcgtcgg acccaggccc gcaccgagcg cgcggagccg gtcgagcgcg
     5281 aggaacagcc gcgcgagggt ctcgccgccg ggctcgtcgg tctggcggtg gacgtcgcac
     5341 agcttggtgt agagcgcatc gaacaggctc ggcccggtgc tgccggtggg cgcgcgggtc
     5401 gcgatctcgt cgaggtagcc gcacagccga tctgcgacgg cgatgacctc gccgtagctg
     5461 gcccggcgca gatagctgat gccgtccagg ccgcgcacga actccatgtc gaagtagaac
     5521 cggccgtcga cctcgccatg accgaggacc ggcggcaccc gcacggcgcc gccgagtccc
     5581 tgcccgaacg ccagctgctt ctcgagctgc cggcgcaggc gcgcgctacc ggccggatct
     5641 cgcgcggcct tgcgcacgaa ccagcgccgg ccgtcgcggg tcatcagcac gacgcgggcg
     5701 ccggacagcc ccgagagagg gcgcgcgtcc gccgcgtcgg acagcagcgg gtcggcgagc
     5761 agcggctcgc tggtcacctg gacctcgccg gagccagcag atccagcacc gccgaggacg
     5821 gcgcctgcgc gcgccggaca tcgaacaccg tcatcccggc ggcgcggccc gccgcgacgc
     5881 catcgtcggt atcctcgaac acgacgcagc gcccgggcgg gaccgcgagc cgctcggcgg
     5941 cgagcaggaa gatgtccggg gcgggcttgc cgcgggtcgc gtcctgcccg gtcacgatcg
     6001 cgtcgaagtg accgcgccag ccgagccgat cgaggatcac ctcgatgccg gagcgcgagc
     6061 ccgacgaggc cagcgcgatc ggcagcgcgc cacgaaacgc cgtgaccagc tcggccgcgg
     6121 ccaggaccgg gaggtcctcg gtgcgcagca gctgcctggc gagctcctgc ttgcgcgcgt
     6181 gcagctcgtc gaccgaccgg gcgcagctgc ggccgcgcag gaaccggggg atcgtctcac
     6241 tcgccttgcc gccgatgttg ccgaagaagt cctcgggggt cagctcgatc ccggcctcgg
     6301 cgaacgtctg ctgataggcg cggtaatgga tcggcatcgt gtcgaccagc gtaccgtcga
     6361 ggtcgaagat caggccgcgc gccgccacgg tttgcatatc ataccacctc aggcgttctg
     6421 ggggtggctg gatcgggccc ctgctcccag ggcgggagat gatcgtcggg acacgcttgt
     6481 tcgaacgtcc cgcgggtcgg ccaggtcaac ggctcgttcg ataccagcat cgcgacggcg
     6541 accaccagaa tggcaatcgc agcccctcgc gccgtcttca gaccaagcac gaccaggcaa
     6601 accaccatga gcatccactg acacgaatag agcattcctt cccatgggtt gaagtaccag
     6661 tagaacgcga aaaacacgcc atagacgatc gccaatccga cggccagcgg ccaggcccct
     6721 ggttccctga cgatccgccg gacggcgtac gcgaccagcg cgagaccgcc gaccagcccc
     6781 agcatggccc acggtgcatg cgccagatgc acgacgtccg agtcgatgta cctgcatatc
     6841 ggctcgatgc cggcagatac ccagctgaac acgatgaagg tcacgacatg gtccgaagtg
     6901 agcgaagcat tggaaagatt gccgaggttc ccccattgac tcgtgtactg caccgcccac
     6961 tgcaggggcg cgtcatggtg caggaagtct ctcgcgacgg aagggagcac tgcgcaagtc
     7021 agcgcgatcg cacacggcat ggcaatgctc agcaatgcgc gagaacacct cgggcgacga
     7081 tcgagccggc tccagacaat ggacgaatag accagcacga tcgccgcggc cgacgtgttc
     7141 gccagaccgg caattcccgc tgcggtaccc gcgaagatcg cgcaccagac cggccgtgcc
     7201 gtgacatggt ccgcgagctc gaggaacagc aggacagcca aggtgctgac cagcgaggtc
     7261 aggatatacg tctcgaacag cgcggtctga aatgcaaagg cgttggtcgc gaagacgaac
     7321 aagcaagatg cgagggcgaa agcgagtgag gcgccgagcc ggatacagag cacgagaaag
     7381 gacgctccga acagtccgac caccagcgcc agggtgttca tggcgctggc tctggcggag
     7441 acccctagcc acttctgtgg ggcggtcagg acagcaaata cgacctgtgc cagcgcgtgc
     7501 ttgcgcgaac ccacgacgcc gtacctgccg gacatgattc ccttgaaatt atcaaggtta
     7561 tccgcaccga atacgatatt ttcaaaatga acggtgtttg cgacgtgcgc gagacgaaga
     7621 tggctgatgg tcgcgaacac gacgcccacc agcacggcga ccgcgatcgt ccaccggatc
     7681 tgatgacgag gcatccgtcg aggtgtacga acgatctcgt gaatcaactg tacgccaccc
     7741 cgaacgctcg aacctatact agacagtgtc attgcggagt ccaaccatag gctgagatat
     7801 tcaggatcat ggaggaggtt gaatgcagcg tgtggatcga gttgcggcgg agtgaccttg
     7861 acggcatgaa atgagtcgcg cggcgggccg gcgtgctttg acgaggcaaa gccgcgatcg
     7921 ccgcgacgtt agcacggcat gcgcggacac cggggcagcg atgcccgacc caccgccccg
     7981 tcgtggtctg cgcacgccga cccgccgatc acgcgggagt gagccaccgt tagctccatc
     8041 tcgtgccacc ctcttcgcaa gccaaggact cgacccgcgc ccgaggcccc tctggcaaaa
     8101 gccgaagtga tacgattggt tatctgatgt ggttctcccg gtggagcggc tgcttcttct
     8161 gtggagtgcc tccctgcagc aatgacgatt tcatgaacgc cgtcctcggc ggcggaacaa
     8221 agtggatcga tgttcgcgat ggctggtccc ggagcaagcg ccacaggggc tcgtctgtcc
     8281 ctgcgtgttg ccggacatac gacggaggtc cgctgatgcc atgggtgctg ttctgcgtcg
     8341 tttcggcggc ctctgccacc atcgggggca ccttgatgcg caaggcagcg ctggccggcc
     8401 cgattctgcc tgaccagtgg ctgccaccac acgtcacgcg ggggatgctc ctgctcggcg
     8461 gggcgctcct ggcctatggc atgtctttca ttggatattt tctggcggtc cggcttgcct
     8521 ccttgaggct ggcctatccg gtcatggtcg ggttcacctg catagggctg tattccgtgt
     8581 ctgtgctcta tttcggcgag ccgcgccggc tgctggatgt cgccggcatc ctcttggtgc
     8641 tggccggtgt cgcgatcctg gctcgcgcgg catagttcca cggcgcgcgc ggcgttccgg
     8701 tcgcgcgaac cgcttgcgga ccgggagacg gcgtccaccg gtgcattggt tgtcgcgtcc
     8761 cgccgggtcg ttcaatcgcg cgcggtgacg gccaccgcgt tttttcccgc aagccacgat
     8821 tctcgctcag cgtacatcgt ggctcggccg gctctcctgc tccttccacc ggcgctcgac
     8881 ccgctcgcgc aacgccgccg ccacgcgggt cccgaccggc ccgagcatca gcagggcgcg
     8941 caacgcccac accttgcggt tggcgcgaag cagctcgggg tacgcggcgc ggccgaagtc
     9001 gatgccccgg cgcgccgtcc gccggtggcc ctgggacagc gcgagcagcc cgcgcagcgc
     9061 gatgtcctgc tcgatgaacg cggcggcgag cacgtcgcgc gtgacgccgg ccttcctgag
     9121 cacctcctcg ggcaggttga acgtggcgat gtactcgtcg gcgaggtgct tgagcgcgcc
     9181 ggagcgctgc tcctgcgcgc cctggccgga gtcgtggacc cggtactcga acagcgggtg
     9241 gtcgatcttg tacgcggtct cggatgccga cagcaccttc cacaggaacc acttgtccgg
     9301 gttcatcaac cgcccgccgg cgtagctgcc gaccgcgtcg tgcagggcct tggggtagca
     9361 cgtggtcagg aacggcagag gcgaacgcag gagctcgagc gagcgcttga gcagcgcggc
     9421 cgcgggcatc gagcgcaccg ggtagccgac cgccctggtc agctcgggct cgtcgcggct
     9481 gtcgcgccag cacttcggat ccggatcgaa ctgcacgccg gtgcgctggc ccttggagtc
     9541 gatcaccgcg gtcgacgatc cccacaccgc ggtgtccgcg cgcggaccca gcgcggtctc
     9601 gagccgcgcg tacgccgcga gcgcgccggg cgtcatcttg tcgtccgacg acaggagcag
     9661 catccgccgg ccgcgcgcca tcgcggcgac gcgctcgagg ttcggcgcga acccgacgtt
     9721 gcacgggttg accgcgagcc ggatccgcgg atcgcgaaag ccgcggacca cgctgaccga
     9781 gtcatcggtg ctcgcgttgt cgcagaccac gatctcgagc tgcgagcgat cgcgtccgag
     9841 atcgcgcccg agatcctgtg cgaggacgct ctcgatcgtc tcgccgatgt accgggcgta
     9901 gttgtagttc gggatcatca ccgagagcgc gacggcgccg tcggggttcg ctgccggatg
     9961 catgctggtc gtcactgcgt gctcctggtt cgccgcgctc gctgccgttc ccgcatagcg
    10021 cgaccgcgga gccaggcaag gcgactgacc aggctaccag cttcgatccg gtgaggcgat
    10081 cgcaaggcga aaccgcagcg atggctgttc gtctgttgca ctcgccacag aacgcccgtg
    10141 gcgatccgga gatgaacccg ccgcaccacc gggcgctccg tatatgtaaa tacttgaaac
    10201 tcgaaggcta acgatgcctc gaggcggcgt tcaccgcagg gcgttgaacg aaccgccgca
    10261 gaccaccgac taacggtcca atgcctaggt tcctgggccg cagtatccgg ctggtcttcg
    10321 acctgggcgt catgtcgctg gcgtactggc tcgcctggct gtttcgcttc gagttccaga
    10381 tcccggccca tgtgctcaag gtcgcgatgc tgacctggcc ctgcgtgatc ggcctgcagt
    10441 atggcacgct cgtcgctttc ggcgtcgagc gcatcgcgtg gcgctacatc aacatgcgcg
    10501 acgcgatgcg ggtgctgacc gcgaccgtga tctcggccac ggtgctcgtc gggttccgga
    10561 tcgcgcacct cctgccggtc accaccagca tcatcccgct cggcgtgctc gcgatggact
    10621 tcgtcctcgg gctggtcggg ctgatcggcg cgcgcgccgc gtggcgcgtg caccacgagc
    10681 tgcgcgctcg ccgccggcac ccgcagaacg gcgatcgcca ccgcgtgttc gtgatcggcg
    10741 ccggcgaggc cggcgtgatg gtcgcccgcg agatcgtgca gcgcccggat ctccacctcg
    10801 agccggtggg cttcctcgac gacgacccgt tcaaggtcgg caagatcgtc ggcggcttcc
    10861 aggtgctcgg catgacccac gaggtcggcg tgctcgccgc gcggctgcgg gtcacgcgcg
    10921 cgctgatcac gatcgccaac gcgtcgggcg ccgacctgcg ccggatcacc gagctgtgcc
    10981 gcgatgccgg gctcgacacc cggatcatcc ccggcctgca cgagatcgtc ggcgacaagg
    11041 tcagcgtgtc gcgcgcccgc gaggtcgcga tcgaggacct gctcggccgc gagccggtca
    11101 agctcgacga ggcggtggtc agtgcgtgcc tgcgcggccg cgtcgtgctg gtgaccggcg
    11161 cgggcggcag catcggctcg gagctgtgcc gccagatctg ccggtttgcc ccccggcgcc
    11221 tcgtgctggt cgagcggttc gagaacgcgc tgttcgagat ccaccgcgag ctcaccgcgg
    11281 cgttccccgg cgtgcggatc gatccgcgga tcgccgacgt ctgcgacatg gaccggctcg
    11341 cccaggtgtt cgagcacacc cggcccgaga tcgtgttcca cgccgccgcg cacaagcacg
    11401 tcgcgatgat ggagtggaac ccgggcgagg cggtcaagaa caacgtcggc ggcacgcggg
    11461 tggtcgcgac gctgtcggat cgctaccagg tcgagcgctt cgtgctggtg tcgaccgaca
    11521 aggcggtcaa cccgtcgtcg gtgatgggcg cgaccaagcg ggtcgccgag atctacctgc
    11581 aggcgttctc gcagcgctcg gcgacccggt tcgtcaccgt gcggttcggc aacgtgctgg
    11641 ggtcggcggg cagcgtggtg ccgatcttcc gccagcagat cgcggccggc ggcccggtgc
    11701 tcgtcaccca ccccgagatg cgccgctact tcatgacgat cccggaggcc agccagctcg
    11761 tgatgcaggc cggcgcgatg ggcgaaggcg gcgagatctt catcctcgac atgggcgagc
    11821 cggtgaagat cgtcgacctc gcgcgcgacc tgatcacgct gtcggggttg cgccccggcg
    11881 acgacatcga gatcgtattc accggcatgc ggccgggcga gaagctgttc gaggagctgt
    11941 cgaccgccgc cgagcacgcc gagaagaccc gccaccccaa ggtgctcatc ggcaagatca
    12001 agccgcacgc ctggaccgag gtgatcgacg gcgtcaacca cctgctcgac gtcgcgggcc
    12061 gagccgaggc tgggatgggt gccgggaccg acgccgggac cgaaacagat ctggtccgcg
    12121 cggcgctgat cgcgctggtg ccggagtgcc ggttcgtgcg cccgcatgtg accgggcgga
    12181 agtcctcgcc gcgcggcgag cgcgcgctga ccgggccgca cgagctggcg ctggcggccg
    12241 gcggcgcgat ccgcaactga gcctctgcgc atgcgcgacc acttcctccc gttcgcgctg
    12301 cccgacatgg acgaggccga ggtcgccgcg gcggccgagg tgatccgctc gggctggctg
    12361 accaccggcg gcaaggtccg cgcgttcgag gccgcgttcg ccgagcgggt cggcgcccgc
    12421 cacgcgatcg cggtcaactc gtgcaccgcg gcgctccacc tcgcgctcga ggcgatcggc
    12481 gtcaagcccg gcgaccgcgt ggtgaccacg ccgtacacgt tcgcgacctc cggcgaggtg
    12541 atccgctacc tcggcgcgca cccggtgttc gtcgacgtcc gccccgacac gctcaacatc
    12601 gatcccgacc gggtcgcggc ggcgctcgcg cgcgaccggg cggcggcgat cctgcccgtg
    12661 cacatcgcgg gcgagccgtg cgacctggac gcgctgcgcg cggtcgccgg cgacatcccg
    12721 atcatcgagg acgcggcgca cgcgctgccg acccggctcg gcgaccggat gatcggcgcg
    12781 ctgtcgcggg tgacctgctt ctcgttctac gcgaccaaga cgctgtcgac cggcgagggc
    12841 gggatgatca cgaccgacga cgacgcgctg gccgaccgct gccggatcat gagcctgcac
    12901 gggatcagcc gcgacgcctg gaagcgctac accgccgagg gctcgtggcg ctacgagatc
    12961 ctcgcgcccg gcttcaagta caacctcacc gacctcgcgg ccgcgctcgg cctggtccag
    13021 ctcggcaagc tcgacgcgat gtggcgccgg cgcgccgagc tcgccgcgcg ctacgaccgc
    13081 gcgttcgccg gctgccccga gctcgagctc ccggcgcgca gcgcgggcaa ccagcacgcc
    13141 tggcacctct acatgctgcg ggtcgacaac gaccggctcg caggcggccg cgacgcgctg
    13201 gtcaccgagc tgcgccggcg caacatcggg gcgagcgtgc acttcatccc gctgcacctc
    13261 cacccgtact accgcgacac ctacggcttc cagccatccg acttcccgat cgcgacccgc
    13321 gagttcgagc gcgaggtgtc gctgccgatc tacagccgga tgacggatcg cgacgccgac
    13381 gacgtgatcg ccgcggtgca cgacagcgtc gcggcgctgc gcaggtgagc gatgcgcctc
    13441 gagccatgcc cgttcgactc cgagcactac ggcctggcga tcggccggct ggtccgcgag
    13501 gccggcgatg gaccgggtga gctcgccgcg gcgatcgcgg ccgcgcggcg cgagcgctac
    13561 gcc
//
LOCUS       DASVOR010000653         1729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_385976,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000653 DASVOR010000000
VERSION     DASVOR010000653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1729
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385976"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF516_12920"
     CDS             complement(<1..180)
                     /locus_tag="VF516_12920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108626.1"
                     /translation="MQVDLERVKHLAREAARLQPGRPALWIGVRAALVVAAPMLIASR
                     IDPLVMTWATLGGFGV"
     gene            690..1616
                     /locus_tag="VF516_12925"
     CDS             690..1616
                     /locus_tag="VF516_12925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108627.1"
                     /translation="MGETDITFRHLLRDHPQPILRLAFSRNRLTPLGTLDPSVDKPMQ
                     RTSDNLFRVRHGAREAAVHIEVEREWRAEIPRRLFEYATSAMVATRLPVWSVVVLLRP
                     GGRPPSGTGVYRIPGPGGDVIQFRYRVVPLWRLDAHRMRKQLGVQGAPYCVAMRGADE
                     AFVRTLAEDVRADRTQPQSDRQSTMQLLYIVSAAILGAETARRIFHMESIIQDPNVQE
                     LINEWKDVGRAEGRTEGRTEGRNEGRTEGRTEGRAVEARLLLHKVLAARSFAVSSEVR
                     TRIDGESDVSRLESWLEAAVTASSIDEVFRDR"
BASE COUNT          272 a          608 c          574 g          275 t
ORIGIN      
        1 gacgccgaac ccgccgagcg tcgcccaggt catgaccagc gggtcgatcc gcgaggcgat
       61 cagcatcggc gcggccacca cgagcgccgc gcgcacgccg atccacagcg cgggccgccc
      121 gggctgcagc cgcgcggcct cgcgggccag gtgcttcacg cgctccaggt cgacctgcac
      181 gttcaccgtg ggctaccacg ggcggcgggc gccgtcccgg agcatcacgg cctgccccgt
      241 ccctcccgtg cgcgcgattc ccacctgcga tcacggcggg agatgtaccg gcggcgcgac
      301 cagcgctccc gccgttctct cgacccctgg cagctgtccg cgaggctggc gctgcggctc
      361 gagcccgctg tggccatcgc tcgcgagtgc acggccagcg cgacgatcca gtgcaccgct
      421 cgcgagcgtg cgcaccttcg gatggcgact cctggtgtcc ggctctcgga ctccatgaac
      481 acgttcgaat cgacgccgct ccgatcatga tgacaagtca tagccccagt ctccagatcg
      541 gcacgaaccc gggccgagcc ttgcgcacct gcgtgcgccg aagctgcaat cggatgtccg
      601 gctatcggac ataccgtccg gatcccggtc gaatcagcgc agcttcatcg tgacagcggc
      661 agacagacga agtacggtga cactcggcga tgggcgagac cgacatcact ttccggcatc
      721 tcctgcgtga tcatccacag ccgatcttgc gcctggcgtt ttcgcgcaac cggctcactc
      781 cgcttggaac gctcgatccc agcgtggaca aacctatgca gcgcacctcc gacaacctgt
      841 ttcgcgtgcg ccacggtgct cgggaggccg cggtgcatat cgaggtcgag cgggaatggc
      901 gagccgagat cccgcgccgc ctgttcgagt atgcgacctc cgcgatggtg gcgacgcgac
      961 tcccggtgtg gtccgtcgtg gtgttgctgc gtccgggcgg gcgtccaccg tcaggcaccg
     1021 gcgtctatcg catccctggt cccggcggtg atgtcatcca gtttcggtat cgggtggtgc
     1081 cgctgtggcg gctcgatgct caccgcatgc gcaagcagct cggcgtccag ggcgctccgt
     1141 actgcgtcgc catgcgaggc gcggatgaag cgttcgtccg cacgctcgcc gaggacgtac
     1201 gagccgaccg cacgcagccc cagagcgatc ggcagagcac gatgcagttg ctgtacatcg
     1261 tctcggccgc tatcctggga gctgagacgg ccaggaggat cttccacatg gaatcgatca
     1321 ttcaagaccc caatgttcag gagctcatca acgagtggaa ggacgtgggc cgagcggaag
     1381 gccgcaccga gggccgcacc gagggccgca acgaaggccg caccgagggc cgcaccgaag
     1441 gccgcgctgt ggaggcgcgc ttgctgttgc acaaggtgct cgccgcgcgc tcgtttgcgg
     1501 tttcctcgga ggtccgcacg cggatcgacg gcgagtcgga cgtctcccgg ctggagtcct
     1561 ggctcgaagc cgccgtcact gcatcgtcca tcgacgaggt gtttcgcgac cgctgaacgg
     1621 ccggcccgga ttttcggccg ccgcgggcgc aaccgtgcta cgccgccgtc gtgaacctgc
     1681 gcgcgtacgc cgagcagatc gtgacgagct gcgcgctcgc ggacaagct
//
LOCUS       DASVOR010000654        17689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_239951,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000654 DASVOR010000000
VERSION     DASVOR010000654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17689
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239951"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="VF516_12930"
     CDS             <1..1293
                     /locus_tag="VF516_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108628.1"
                     /translation="CQSKGMPATTLTGTVFAPNGTLPLFGVSVYVPNSAPGAFQPGVQ
                     CGSCGDALPGDPIVQVVTDEAGQFVLPQVPDGADIPVVITIGKWRRQIRLARVAACTT
                     QTIEAADTTLPKSRDDLTPATTSVDLPQIAISTGSADSLECLVRRLGIADPEITTDQQ
                     GGKIHLFADTGATVDAVGVGVSSFDAGFRGGAGAFSDSQTLWGTAGDPGKLNDYDIVI
                     LSCEGGQHVETKSQQAMDHLKAYADRGGRVFLSHWHNIWIEGSTSGRNANATKPAVWP
                     GLAQWPNDSPTDPTADTIDETHNPKGPSFATWMLNVGGSPPGMRDAIPLQDGTGKDTC
                     STVDSTSVEQWVYWQMDAQTQMAQDFQFTTPIEQPLGDRCGKVVFSDMHVSGGPVTNG
                     TMIPPYPTSCGTETELSPQEKALAFMFFDISSCVGGLF"
     gene            1489..7320
                     /locus_tag="VF516_12935"
     CDS             1489..7320
                     /locus_tag="VF516_12935"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8108629.1"
                     /translation="MTTGDKIDGRPARILGELEQRIASAGSAEDRLTAAAELHHKFSF
                     DEVAGGDECLAYEPRCTTSCEQCQTMWIGTKAGEVLRLQLGGASVRRQKAMFQSGVRA
                     LAHVHHLGQLLVGLDIGYVVVLDEGLEDYGSAGWQCHPTAALGNHRGHEVPSEPLRYE
                     PGFAIEDPAQPERFMHGITAIRELPGAAGDRGVDAVIFTRAPELLVVRLARDAMTIRG
                     RHPLPGWGRYLTIAGPVGDETLVCVTRSGELREWRCRALRAAPCDPTPMYEDRMNLLP
                     TALATPSGPQRNQFALFLGTSDGLYIRRVRGERPVHVPVTRSAVLSIATVSLPGQGRA
                     DPKHYVALGLEDGRLRVLEEQLLFAWLDGKDAPLAHDFRVALGDAVLGLEIMAPRVPT
                     SRFVLAGLRDHRLRLFHVRSRESLLDDVRARWRAAIGEPAVLVRDQLEAQLTALLVNE
                     AALRERTGTGREHALRYLLVDDVLPRWTACLGASDPRIVQRACELARGADDKVLYRLS
                     ATMGDIAGGDANALIRISQACLVAMPQRDPQRWRAFVSYHLKRFHDCATRPELAADLP
                     RLQHWSRFVRKFLLLGETFADKRVRIKTLMDRNCATHKYLDALIYAAQLEQQRYDLKW
                     RKVAHRDQQGRAQEIARVEIIDKVAIVITAAAEIVFFDLDGTSLPIFEGGHERPCLTP
                     DRGARSSGAIRTRVARVTRKQGTWVRIALSWASGVKSDQPRLTVFDVQFGNRRVDVDG
                     RHAVTTWPPRADGAEIEIHGLFGLPGADAFLVGLDSSESPLALATWDASETGAHRWVL
                     SELSRGDAQRTGPPAVGSTPVRAVAALELEDAPGCYLAAAGAADGSLRFTVFDAGHEE
                     HEIVASDDGPTLLVHPINDIALTVRRGDPEHYGYVCYAGTETGESVCLRVKVDRKAGT
                     IVARQLWRDLHDSPVIAVRPTKKLEPRSPAMYGDDVVFIVTQDGHIAIHRAARSDVED
                     DAVSASNNYYFEGMRFDRINLPKGMTSWASPRSRSGFLAAYPDGELLYGELHAPRESN
                     AHAGMLRVTDQIYAKVMQSQVFHGVDDGVDDKVAEDRKLAICAMVRIGGGALRNYVLR
                     KQLGRVAWNAIDEVGLGKVLDEHFAGLSAGIPEERDRIKLVVKTVSSRVLDLSPTEIL
                     EHCRPGKAYDRIAVYPRVRRACQYLADYLLDAAVQSQRGAVRVRMSIMHALFRANVLW
                     HAALDEQSGRHITSALRDILITCLRDENRIVCVEALRAVAVVLRNITVLLRRARPEDH
                     PEHHRAIRTSFFPRGLTTMQWLVGTILENFTRYRRHSHPVTWSYNTVLVLMFRLFPGD
                     ALGLCEQISQRGLGDHLEAILDRLRGRTVRALRSRIRELWILPLPRQRAQARAEFIDR
                     FADCNISQRLLEYEIATDDSDYIRASGLLHIYRCLALLWKVNHTADITDLPSRWARVA
                     GGPELTGSLAGMTRILDKFVEISRARPEDRVKLLTQVTNLFPDTQSPPEAVRLVLERI
                     VEDWQAIVEPRVPSQGDEVLERRLGAMVAERSSRLVYAVKDDPTRVVTVLRYPGNDTA
                     RLEVNRNARLGRELAERYPESFVAVHALDPTSGCALVDSAMVARDFLAELKVMDPNER
                     SAIAQRAAVQLARALRCLHAEGLNHGDLRAENVLVRNAGGTAIFQLGDLRAVPSGIED
                     SVAALRIPGVLGAAPDGAGKLENWIDLMSLTLFLHEVLTGGKLNPRADRSALADVAAA
                     LRVRGDDVSHVIARMIAGEPCKTVEEFEQAIRSDRPEFEHRPLVGRVAAEFDVFISYD
                     SHQSESVAAELDAHLMRRGIMPFFARRDAVPDRWWDVIERALKRSRAFAILVGDDYRI
                     HGRQHEEVQAARPAARSERLPFHVFLLGASFPPEFWQDPVIRLGRDAEAMAKHIARSF
                     QHPISYPGAAPSADPDLAGPGPSGTGGATGPGTAGGEDEGEPG"
     gene            7407..8663
                     /locus_tag="VF516_12940"
     CDS             7407..8663
                     /locus_tag="VF516_12940"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HEX8108630.1"
                     /translation="MTGKEQYHVFLSYRSVNRTWVLNLYDVLHQQGFEVFLDQVALTG
                     GDELIKKLEQGLTHSQAGILIWSSASADSDWVRKEYQTLERQATKSKSFRFVPVLLDA
                     SELPAFAADRVFLDFRSYPDGPNGGELLRLLYSIVGQPLTPEAAHFAAEQDAVAKQQA
                     NEVVAAVHLKDHERLQELFAAGGLPWETSSALGCKAAEGLIELGKNTEAIAMLEALRA
                     RFPRAIRPKQLHALALARRAHGSDLRDAQRILAALYADGERDPETLGIYGRTWMDRFT
                     LSQDRADLEQSRNLYAEAFEKATDDYYTGVNAAAKSVFLGTPEDLERAEDYARRVQDI
                     VGTTPRQGDYWMTATVGEVLLMQKNYRDAASVYKAGVAMALAKTASHLSTWRQAHRLM
                     EKLQPSPEERTLIRNAFAHLPDPPPP"
     gene            8802..10187
                     /locus_tag="VF516_12945"
     CDS             8802..10187
                     /locus_tag="VF516_12945"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8108631.1"
                     /translation="MTTSVYVVSDLHLGGAPGFRMCSEQGVKRLAALFEHISRQAGQG
                     SRIELVIAGDIVDFLAVEDSGGGGTWSAFTNKHQDALDKLEEILESTAPVWDGLAACA
                     RRGCIVTLMLGNHDLELSFPRLRRRLIEKLGGGPIMFLYDNEAYTNGKLLVEHGNRYD
                     SWNTVDHDGLRRVRSLLSRNQAAAEFATQPGSELVVRVMNKIKRDYAWVDLLKPETGA
                     AIPILAVLATGLWRKAGPAIVEAARAAWRDRVFRADRMPRDDSGYVAELDPEAPVAEP
                     AFPDEDVLAWVEQNVPRDADGSIGALADIEERLLFAAMRKWGEKDSRTFAIENEDERY
                     LDAASTLARGGYSVVIFGHTHLVKRIALQDAPGATYLNTGTWADLIRVPESTLTGEQD
                     AARTEFHEFFQDLEQNRIERIRRLIPTFARVDLDDRGEVVQEGVYFFDEGGAITEVTT
                     AGVLAHLKAAP"
     gene            10184..12109
                     /locus_tag="VF516_12950"
     CDS             10184..12109
                     /locus_tag="VF516_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HEX8108632.1"
                     /translation="MIDFEAQPAVKRATARLKAGRWYGTGYLVRPDRVATCYHVVKDL
                     AAGTPIQCTFADDAHTTRTAVVDVVDADADCALLKLELAMTVDPLRLSSARPAFHFWT
                     FGFPAFTAGVGLSIGAELLDPDTVSAEGHRAFSMYSKQFAGDLPTTMGGLSGSPVLVG
                     GVVVGHMSSVLGAMDAAQVPHLGQAYAVGAAGVMRLLGEPTVSMAQPPVRDRKAAEAA
                     EVTVANQARVFLEIEASASGTEVNAALERGRVHGAVSDEALLFAGNRLLELDLIDDAI
                     AMVERVPETPQRQRDLAVAYSLRGDHAQAQALLKPLTDSHSRAIEGGTLKRRWLETGK
                     TMWLQAACDAYEAGYAIDGDHYPGINAAYCAFELGRRSDGERIAREIIARLQPIATRD
                     KWQEASLAEAHLLTGDLEAARVRYQAAAKKHLAQWRNLAIMRRQARKILELLGQPRAS
                     FDAIFPVPATAAFTGHRVDPDETRGRFAPSRVDGVKRRLARALEERDVQFGFSSAADG
                     GDILFLEALLERGGEAHVYLPFPADAFASSSVAQAWQDRFHALIQKLGRRVVVLSDQM
                     PESGPLRDAGFANCNAAIQEAARHHAGTLDEPPLLIAVVAGDGTDGTGGVGGAHDAVR
                     RWIDRGDRVFQIDPRTV"
     gene            12309..12806
                     /locus_tag="VF516_12955"
     CDS             12309..12806
                     /locus_tag="VF516_12955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108633.1"
                     /translation="MTKLAIEGTCLCKAVEIGVTRRPRQVTQCNCSVCRRYGTLWAYY
                     RRSAVSITAPRGALEDYSVRAGGLKFVRCRSCGCVICWDRPGGGRDQRMGINTRLLDH
                     AQMADVPVKVLDGDQSWRVLGVYVKPEMWVSPSRVEKQPHYQRPASTSRRAPRSRPRS
                     RATAR"
     gene            13612..14997
                     /locus_tag="VF516_12960"
     CDS             13612..14997
                     /locus_tag="VF516_12960"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8108634.1"
                     /translation="MKSSIRSKVRHRTGTLAVCSGVLLAGGIGAGCADAPDDREPVEF
                     ASTEQSVTVAGDPLPGITATDFAAAKANFAAVEGLPDGLGPIFNEKACGNCHTQGAIG
                     GAGVQIERRFGRFDNGIFNSLSNEGGSLRQLFTVGAFTGLGGQACNPALEHEPADATV
                     HNVGRLTTPLFGAGLIDTIPDSVIIANANAQPLSVRGTVNNVKVLLPNPADPTQVLNG
                     TRVGKFGWKAGIAALAQFAADAYVNEMGITTQHCIGGTSVTAFANESKPNGIAVPAGC
                     DDQAPPHAAIAGVPANTDDVVGACAPGQSELQDDVANFLKFMTFLAPVPRLAIDPNVN
                     LQGGTVFNAIGCADCHLLKDYNTGPNPPNGVPANMLFRPRTDFLLHDIGTGDMIGNDG
                     DTLARTKMMRTAPLWGLHVRTKFMHDGSQTSIVGAINAHGGQAATAAFNFFFGISASD
                     QNAMLTAMMSD"
     gene            complement(15021..16661)
                     /locus_tag="VF516_12965"
     CDS             complement(15021..16661)
                     /locus_tag="VF516_12965"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8108635.1"
                     /translation="MTAAHDPTAPPERASPADAVDVLIVGAGFGGLYALYRLRGIGLT
                     AVVLEAAPSVGGTWWTNRYPGARVDIQSLEYSYSFSEPLQQQWRWTERYAGQPELLRY
                     ANHVADRFDLRRDIRLGTRVAAAHFDEAARRWRVVSDAGAAWTARFLVLAIGPLSSPN
                     VPAFPGLDRFAGPVLHSAAWPDPPVDLSERHVAVIGTGSSGVQLIPILAEQARALTVF
                     QRTAAYAVPARNGPLDPAWEARVKADYAGFRARNRKMFSGFGGELSAAPMSALDATEA
                     QREAVFEERWRIGGFSFLGAFRDLLTSPGANELAAEFVRRKIRQIVRDPATAALLCPR
                     YPIGCKRMAVDTGYYETYNRSNVALVDIATQPIEAITPGGLVAGGREHRFDVLVLATG
                     FDAFTGPVVRIDLRGRGGLRIQDKWRAGPVNYLGLTVAGFPNLFYVAGPGSTSAFTNF
                     FVAVEHHVDWIAGCVAWLDARGHATIEASEDAESRWVAFVNAAADRTVYPNCQSWYLG
                     ANVAGKPRVFMPLAAGFPAYAERCAAVASRDYDGFAVG"
     gene            complement(16847..>17689)
                     /locus_tag="VF516_12970"
     CDS             complement(16847..>17689)
                     /locus_tag="VF516_12970"
                     /inference="COORDINATES: protein motif:HMM:NF015857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HEX8108636.1"
                     /translation="RAEPAAGGLAPVVDAPAPVTEAPAVSALAPSPPRPRFYVRLGGA
                     LVKPVSSSRELELADVHGAATLAVHNGPIAGSGATIDSATIPAAILGWVPRTASRRWS
                     GELVLGLPFTVKFRATGTLATTSLAPMALGIPTGIQPLGPDLGEAQAAPVVLTATYRL
                     IDHRYVWPYLGIGPSVLFARNARVTNPILTEVNHPEMSIDPAPGLVLQGGLDARLSRS
                     IYARIDVKFIAFMLARAEVQHVNVKTPGLPLFDSVEVGTAKMSLWVNPFIVQACLGAD
                     FDLW"
BASE COUNT         2830 a         6004 c         6265 g         2590 t
ORIGIN      
        1 tgccagagca aggggatgcc ggcgacgacg ctcaccggga cggtgttcgc gcccaatggc
       61 acgctgccgc tgttcggggt cagcgtgtat gtcccgaact ccgcccccgg cgcgttccag
      121 cccggggtcc agtgcggcag ctgtggcgac gcgctgcccg gcgatccgat cgtgcaggtc
      181 gtcaccgacg aggcggggca gttcgtcctg ccccaggtgc ccgatggcgc cgacatcccg
      241 gtggtgatca cgatcggcaa gtggcggcgc cagatcaggc tcgcccgcgt cgccgcgtgc
      301 acgacccaga cgatcgaggc cgccgatacc acgcttccga agagccgcga cgatctcacg
      361 cccgccacca cgtcggtcga cctgccgcag atcgcgatct cgaccggcag cgccgattcg
      421 ctcgagtgcc tcgtgcgccg gctcggcatc gccgaccccg agatcacgac cgaccagcaa
      481 ggcggtaaga tccacctgtt cgcggacacc ggcgccacgg tggatgccgt cggcgtcggg
      541 gtcagcagct tcgacgcggg cttccgcggc ggcgccgggg cgttcagcga ctcgcagacc
      601 ctgtggggaa ccgccggcga ccccggcaag ctgaacgact acgacatcgt gatcctgtcg
      661 tgcgagggcg ggcagcacgt cgagaccaag tcgcagcagg cgatggacca cctgaaggcc
      721 tatgccgatc ggggcggccg cgtgttcctg tcgcactggc acaacatctg gatcgagggc
      781 agcacctcgg gcaggaatgc caacgcgacc aagccggcgg tctggccagg tctcgcgcag
      841 tggccgaatg acagccccac cgacccgacc gccgatacga tcgacgagac ccacaacccg
      901 aagggcccat cgttcgcgac ctggatgctg aacgtcggcg gctcaccgcc cggcatgcgc
      961 gacgcgattc cgctccagga cggcaccggc aaggacacct gcagcaccgt cgacagcacc
     1021 agcgtcgagc agtgggtgta ctggcagatg gacgcccaga cccagatggc ccaggacttc
     1081 cagttcacga cgcccatcga gcagccgctc ggcgatcgct gcggcaaggt cgtgttctcc
     1141 gacatgcatg tctccggcgg cccggtcacg aacggcacca tgatcccgcc gtatcccacg
     1201 agctgcggca cggagaccga gctgtcgccg caggagaagg cgctcgcctt catgttcttc
     1261 gacatctcgt cctgcgtcgg cggcctgttc tgagcgcacc gccgcggtgc cgtggccgga
     1321 acggaccagc cggtcttacc gctttgacgc cgcgcggcgg atctcctctg cccgggtgac
     1381 gcatgtcgcg gcccggaggg cggtatcggt agatcgcact cacgatgtcg tggtggcctg
     1441 gccatcttcg ccatatccgg gcggcaagcg gaccgccgga ggttggcgat gacgacaggt
     1501 gacaagatcg acggtcgacc ggcgcggatc ctcggcgagc tggagcagcg gatcgcgagc
     1561 gcggggtcgg cggaagaccg cctgacggcg gccgcggagc ttcatcacaa gttctcgttc
     1621 gacgaggtcg ccggcggcga cgaatgcctc gcctacgagc cacggtgcac cacgtcgtgc
     1681 gagcagtgcc agacgatgtg gatcggcacg aaggcgggcg aggtgctccg cttgcagctc
     1741 ggtggcgcga gcgttcgccg ccagaaggcg atgttccaga gcggtgtccg cgcgctcgcg
     1801 cacgtgcacc atctcggcca gctcctcgtc gggctggaca tcgggtacgt ggtggtgctc
     1861 gacgagggcc tcgaggacta cggcagcgcg ggctggcaat gccaccccac cgcggcgctc
     1921 gggaaccatc gcgggcacga ggtcccgagc gagccgctgc gctacgagcc ggggttcgcg
     1981 atcgaggatc ccgcgcagcc ggagcgcttc atgcacggga tcacggcgat ccgggagctt
     2041 cccggcgccg ccggggatcg aggtgtcgac gcggtgatct tcacgcgagc gccggagctg
     2101 ctcgtcgtgc ggctggcgcg cgacgccatg acgatccgcg gccgccatcc gctgccgggc
     2161 tggggccgct acctgaccat cgccgggccg gttggcgacg agacgctggt ctgcgtcacg
     2221 cgcagcggcg agctccgcga gtggcggtgc cgcgcccttc gcgccgcgcc gtgcgatccg
     2281 accccgatgt acgaggatcg catgaacctg ctgccgacgg cgctggccac gccgtccggc
     2341 ccccagcgga accagttcgc gctgtttctc gggaccagcg acgggctgta cattcgccgg
     2401 gtacgcggcg agcgcccggt gcacgtgccg gtgacgcgca gcgccgtgct gtcgatcgcg
     2461 acggtgtcgc tcccggggca gggccgcgcc gaccccaagc actacgtggc gctcggcctc
     2521 gaggacggcc gcctccgcgt cctcgaggag cagctgctgt tcgcctggct cgacggcaag
     2581 gatgcgccgc tggcgcacga cttccgggtc gcgctcggcg acgcggtgct cggcctcgag
     2641 atcatggcgc cgcgcgtgcc gacctcgcgg ttcgtgctcg ccgggctgcg cgatcaccgg
     2701 ctgcggctgt ttcacgtccg cagccgcgaa tcgctgctcg atgacgtgcg cgcgcggtgg
     2761 cgagcggcga tcggcgagcc ggcggtgctc gtccgggatc agctcgaggc gcagctcacc
     2821 gcgctgctcg tcaacgaggc cgcgctgcgc gagcggaccg gcaccggacg cgagcacgcg
     2881 ctgcgatacc tgctggtcga cgacgtgctg ccgcggtgga cggcgtgcct gggagccagc
     2941 gacccgcgca tcgtccagcg cgcctgcgag ctcgcgcgcg gcgccgacga caaggtcctg
     3001 taccggctgt cggcgaccat gggtgacatc gccggcggag acgccaacgc gctgatccgg
     3061 atcagccagg cgtgcctggt cgccatgccg cagcgcgatc cgcagcgctg gcgggcgttc
     3121 gtgtcgtacc acctcaagcg atttcacgac tgcgcgacgc gccccgagct cgcggccgat
     3181 ctgccccggc tgcagcactg gagccgcttc gttcgcaagt tcctgctgct cggcgagacg
     3241 ttcgccgaca agcgggtgcg gatcaagacc ctgatggacc gcaactgcgc gacccacaag
     3301 tacctcgacg cgctgatcta cgccgcgcag ctcgagcagc agcgctatga cctcaagtgg
     3361 cgcaaggtcg cgcaccgcga ccagcagggt cgcgcgcagg agatcgcgcg ggtcgagatc
     3421 atcgacaagg tcgcgatcgt gatcaccgcg gcggccgaga tcgtgttctt cgatctcgac
     3481 ggtacctcgc tgccgatctt cgagggcggc cacgagcgcc cctgcctcac gccggatcgc
     3541 ggggcccgat cgtcgggagc gatccgcacc cgcgtggcgc gggtgacccg caagcaaggg
     3601 acgtgggtcc ggatcgcgct cagctgggcg agcggggtca agtcggacca gccccggctc
     3661 accgtgttcg acgtgcagtt cggcaaccgg cgggtcgacg tcgacggacg gcatgcggtg
     3721 accacctggc cgccgcgcgc cgacggcgcg gagatcgaga tccacggcct gttcggcctg
     3781 cccggcgccg atgcgttcct ggtcgggctc gactcgagcg agtcgccgct ggccctggcg
     3841 acctgggacg catcggagac cggcgcgcac cgctgggtgc tgtcggagct gtcgcgcgga
     3901 gacgcgcagc gcaccggccc gcccgcggtg gggtcgacgc cggtgcgcgc cgtggcggcg
     3961 ctcgagctgg aggacgcgcc cggctgctac ctcgcggcgg ccggcgcggc cgatggctcg
     4021 ctgcgcttca cggtgttcga cgccgggcac gaggagcacg agatcgtggc cagcgacgac
     4081 gggccgacgc tgctcgtcca cccgatcaac gacatcgcgc tcaccgtgcg ccgcggcgac
     4141 cccgagcact acggctacgt gtgctacgcg ggtacggaga ccggcgagag cgtgtgcctg
     4201 cgggtgaagg tcgatcggaa ggccggcacg atcgtcgcca ggcagctgtg gcgcgatctg
     4261 cacgacagcc cggtgatcgc ggtccggccg accaagaagc tcgagccgcg cagcccggcg
     4321 atgtacggcg acgacgtcgt gttcatcgtg acccaggacg gccacatcgc gatccaccgg
     4381 gcggcgcgca gcgatgtcga ggacgacgcc gtcagcgctt ccaacaacta ctacttcgag
     4441 ggcatgcgct tcgatcggat caacctgccg aagggcatga cgtcgtgggc gagcccgcgc
     4501 agccggtcgg ggttcctcgc cgcatacccc gacggcgagc tgctctacgg cgagctccac
     4561 gcgccgcgcg agtcgaacgc ccacgccggc atgctgcgcg tgaccgatca gatctacgcc
     4621 aaggtcatgc agagccaggt gttccacggc gtggacgacg gcgtcgacga caaggtcgcc
     4681 gaggatcgca agctcgcgat ctgcgcgatg gtccggatcg gcggcggcgc gctgcgcaac
     4741 tacgtgctgc gcaagcaact gggacgggtc gcgtggaacg ccatcgacga ggtcggcctg
     4801 ggaaaggtgc tggacgagca tttcgccggc ctcagcgccg gcatccccga ggagcgcgac
     4861 cggatcaagc tggtcgtgaa gacggtgtcg agccgcgtcc tcgacctgtc ccccacggag
     4921 atcctcgagc actgccggcc cggcaaggcg tacgaccgga tcgccgtgta tccccgcgtg
     4981 cggcgggcct gccagtacct ggccgattac ctgctcgacg ccgccgtgca gtcgcagcgc
     5041 ggcgcggtcc gggtccgcat gtcgatcatg cacgcgctgt tccgcgccaa cgtgctgtgg
     5101 cacgcggcgc tcgacgagca gagcgggcgc cacatcacga gcgcgctgcg cgatatcctg
     5161 atcacgtgtc tgcgcgatga gaaccggatc gtctgcgtcg aggcgctgcg cgcggtggcg
     5221 gtcgtgctcc gcaacatcac ggtgctgctc aggcgcgcca ggcccgagga ccatccagag
     5281 caccatcggg cgatccgcac cagcttcttt ccgcgcggcc tgaccaccat gcagtggctg
     5341 gtcggcacga tcctcgagaa cttcacgcgt taccggcgcc acagccaccc ggtgacgtgg
     5401 tcctacaaca ccgtgctcgt gctgatgttc cggctgttcc cgggcgacgc gctcgggctg
     5461 tgcgagcaga tctcgcagcg tgggctgggc gaccacctcg aggcgatcct cgatcgcctg
     5521 cgcggccgga ccgtgcgtgc gctgcgctcg cggatccgcg agctgtggat cctgccgctg
     5581 ccgcgccagc gggcgcaggc gcgagcggaa ttcatcgatc ggttcgctga ttgcaacatc
     5641 agccagcgtc tgctcgaata cgagatcgct accgatgact ccgactatat ccgcgcgagc
     5701 gggctgttgc acatttaccg ctgcctcgcg ctgttgtgga aggtgaacca cacggcggac
     5761 atcacggatc tcccgtcgcg atgggcccgt gtcgccgggg gaccggagtt gaccggcagc
     5821 ctcgcgggca tgacccgcat cctcgacaag tttgtcgaga tctcccgcgc ccgcccggag
     5881 gaccgggtca agctgctcac ccaggtcacg aacctgttcc ccgataccca gagcccgcca
     5941 gaggcggtgc ggctcgtgct cgagcgcatc gtcgaggact ggcaggcgat cgtcgagccc
     6001 agggtcccga gccagggcga cgaggtcctc gagcgcaggc tcggcgcgat ggtcgccgag
     6061 cgcagcagca ggctggtgta cgcggtcaag gacgatccga cgcgcgtcgt caccgtgctg
     6121 cgctatccgg gcaatgacac cgcgcggctc gaggtcaacc gcaacgcgcg gctcggccgc
     6181 gagctggcgg agcgctaccc ggagtcgttc gtcgcggtcc atgcgctcga tccgacctcc
     6241 ggctgcgcgc tggtcgacag tgccatggtc gccagagact tcctcgccga gctcaaggtc
     6301 atggacccga acgagcgctc ggcgatcgct cagcgcgccg cggtgcagct ggcgcgcgcg
     6361 ctgcgctgcc ttcacgccga gggcctgaac cacggcgatc tgcgcgccga gaacgtgctc
     6421 gtccgcaacg ccggcgggac cgcgatcttt cagctcggcg atctgcgggc cgtcccgagt
     6481 ggtatcgagg actcggtcgc ggcgctgcgg attcccggcg tcctcggcgc cgcgccggac
     6541 ggtgccggca agctggagaa ctggatcgat ctgatgtcgc tgacgctgtt cctccacgag
     6601 gtgctcaccg gcggcaagct caatccgcgc gcggaccgca gcgcactcgc ggacgtcgcg
     6661 gcggcgctgc gggtccgcgg cgacgacgtc tcgcacgtga tcgcacgcat gatcgccggt
     6721 gagccatgca agaccgtcga ggagttcgag caggcgatcc gatcggaccg cccggagttc
     6781 gagcatcggc cgctggtggg ccgcgtcgcc gcggagttcg acgtcttcat ctcctacgac
     6841 tcgcaccaga gtgagtcggt ggctgccgag ctcgacgcac atctcatgcg gcgcggtatc
     6901 atgccgttct tcgcgcggcg cgatgcggtt cccgatcgct ggtgggacgt gatcgagcgc
     6961 gcgctgaagc gcagccgggc gtttgcgatc ctggtcgggg acgactaccg cattcacggc
     7021 cgccagcacg aagaggtcca ggctgcgcga ccggcagcgc gcagcgaacg gctgccgttc
     7081 cacgtgtttc tgctcggcgc cagctttccc ccggagttct ggcaagaccc ggtgattcgc
     7141 ctcggccggg acgccgaggc catggcgaag cacatcgctc gcagcttcca gcacccgatc
     7201 tcgtaccccg gcgctgcgcc gtccgcggac cctgatctcg cgggccccgg gcccagcggg
     7261 accggcgggg cgaccggacc cgggactgcc ggcggtgagg acgagggcga gccgggctga
     7321 ggcccgtgga tgagagtacg ctagatcaac gagggggaaa catgacatcc gctgaatggc
     7381 tgcgactggc acccagaccc cggcccgtga ccgggaagga gcagtatcac gtgttcctgt
     7441 cgtaccggtc cgtgaatcgg acctgggtgc tcaatctgta cgacgtgctc caccagcaag
     7501 gcttcgaggt gtttctcgat caggtcgcgc tgaccggcgg cgacgagctc atcaagaagc
     7561 tggagcaagg gctgacacat agccaggccg ggatcctgat ctggtcatcg gcgagcgcgg
     7621 attccgactg ggttcgcaag gaatatcaga ccctcgaacg acaggccacg aaaagcaagt
     7681 cgttccggtt cgttccggtc ctgctcgacg cctcggagct tcctgcgttt gccgccgacc
     7741 gcgtgttcct cgacttcagg agctaccccg acggccccaa tggcggcgag ttgctgcggc
     7801 tgctgtactc catcgtcgga cagccattga cgcccgaagc cgcgcatttc gccgccgaac
     7861 aggacgcggt cgcgaagcag caggcgaacg aggtggtcgc cgccgttcac ctcaaggatc
     7921 acgagcgcct tcaggagctg ttcgcggccg gtgggctacc gtgggaaacc tcgtcggcgc
     7981 tgggctgcaa ggccgccgag ggcctgatcg agctcggcaa gaacaccgag gcgatcgcca
     8041 tgctcgaagc gctgagggcg cgattcccgc gcgcgatccg gcccaagcag ctccacgcgc
     8101 tcgcgctcgc ccggcgagcc cacggcagtg acctccggga cgcgcagcgc attctcgccg
     8161 cgctctacgc cgacggcgag cgcgaccccg agaccctggg catctacggg cgcacgtgga
     8221 tggaccgttt caccctgtcc caggatcgcg ccgacctcga gcaatcgcgg aacctctacg
     8281 ccgaggcgtt cgaaaaggcg accgatgact actacaccgg cgtcaacgcg gccgccaaga
     8341 gcgtgtttct cgggacgccc gaagatctcg agcgtgcgga ggactatgcc aggcgtgtgc
     8401 aggacatcgt ggggacgacg ccgcggcaag gcgactactg gatgaccgcg acggtcggcg
     8461 aggtgctgct catgcagaag aactaccgag acgccgcgag cgtgtacaag gcgggagtcg
     8521 cgatggcgct ggccaagacc gcgtcacatc tcagcacgtg gcggcaggcg catcggttga
     8581 tggagaaatt gcagccatcg cccgaggagc gcacgctgat ccggaatgcg ttcgcgcatc
     8641 tgcctgaccc tccgccgccg tgacgccagc gcggcgcgaa ccgcggaggc ctcgggtcac
     8701 agggcggccg atttgcgtgc cgcgaagccc atcagctgca tcggaaccga tacaatgatc
     8761 gatgcgctcg cggcagtggc tggcgagcgg gaggagctgc gatgaccacg agcgtgtacg
     8821 tcgtatccga tcttcatctg ggaggcgctc ccggattccg catgtgctcc gaacaggggg
     8881 tcaaacggct cgccgcgctg ttcgagcaca tctcccggca ggccggccag ggctcgcgca
     8941 tcgagctcgt gatcgccggg gatatcgtcg acttcctcgc ggtcgaggat tcgggcggcg
     9001 gtgggacgtg gagcgcgttc acgaacaagc atcaggacgc gctggacaag ctcgaggaga
     9061 tcctcgagag caccgcgccg gtgtgggacg ggctcgccgc gtgcgcccgc cggggatgca
     9121 tcgtgaccct gatgctcgga aaccacgatc tcgagctgtc gtttccccgg ctgcgccggc
     9181 ggttgatcga gaagctcgga ggcggaccga tcatgttcct ctacgacaac gaggcctaca
     9241 cgaacggcaa gctgctcgtc gagcacggca atcgctacga cagctggaac accgtcgatc
     9301 acgacggtct gcgccgggtc cgctcgctgc tgtcgcgcaa ccaggcggcg gccgagttcg
     9361 cgacgcagcc gggcagcgag ctggtcgtcc gggtgatgaa caagatcaag cgggactacg
     9421 cctgggtcga tctgctgaag ccggagaccg gcgcggcgat cccgatcctc gccgtgctcg
     9481 ccaccgggct gtggcgcaag gccggcccgg cgatcgtcga ggctgcgcgc gcggcctggc
     9541 gtgaccgggt gttccgcgcg gaccggatgc cgcgcgatga cagcggctat gtggccgagc
     9601 tggatccgga ggccccggtg gccgagccgg cgttccccga cgaggacgtc ctcgcctggg
     9661 tcgagcagaa cgtccctcgc gacgcggacg gctcgatcgg cgcgctcgcc gacatcgagg
     9721 agcgcctgct gttcgccgcc atgcggaagt ggggagagaa ggactctcgc acgttcgcga
     9781 tcgagaacga ggacgagcgc tacctcgacg ctgccagcac cctcgcgcgt ggcggctatt
     9841 cggtcgtgat cttcggccac acgcacctcg tgaagcgcat cgcgctgcag gatgctccgg
     9901 gcgcgacgta tctcaacacc gggacctggg cggatctgat ccgggtgccc gaatcgaccc
     9961 tcacgggcga acaggacgcc gcccggaccg aattccacga gttctttcag gatctcgagc
    10021 agaaccggat cgagcgcatc cgccggctga tccccacgtt cgcgcgcgtc gatctcgacg
    10081 accgggggga ggtcgtccag gagggcgtgt acttcttcga cgagggcggc gcgatcaccg
    10141 aggtcacgac cgcgggcgtg ctcgcccacc tgaaggcggc gccatgatcg acttcgaagc
    10201 ccagcccgcg gtgaagcggg cgaccgcgcg cctcaaggcg ggtcgctggt atggcaccgg
    10261 ctatctcgtg cgacccgatc gggtcgccac ctgctatcac gtcgtcaagg acctcgccgc
    10321 tggtacgccg atccagtgca cgttcgcgga cgacgcgcac accacccgca cggccgtggt
    10381 ggacgtggtc gatgcggacg ccgactgcgc gctgctgaag ctcgagctgg cgatgaccgt
    10441 ggacccgctg cggctcagca gcgcgcggcc cgcgttccac ttctggacgt ttggctttcc
    10501 ggcgttcacc gcgggcgtcg ggctctcgat cggcgccgag ctgctcgatc cggacacggt
    10561 cagcgccgaa ggccaccgtg cgttctcgat gtactcgaag cagttcgccg gggacctccc
    10621 cacgacgatg ggtggactgt ccgggagccc ggtgctcgtc ggtggcgtgg tggtcggtca
    10681 catgtcgagc gtgctgggcg cgatggatgc cgcgcaggtg ccgcatctgg gccaggcgta
    10741 cgccgtgggt gccgccggcg tgatgcggct actcggcgag cccaccgtga gcatggcgca
    10801 gcctcccgta cgcgatcgaa aggcggcgga ggcggccgag gtcaccgtcg caaaccaggc
    10861 gcgggtgttc ctcgagatcg aggcctcggc gtcgggcacc gaggtcaacg ccgcactcga
    10921 gcggggacgc gtccacggcg cggtgagcga tgaggcgctg ctgttcgcag gaaaccggct
    10981 cctggagctg gacctcatcg acgatgcgat cgcgatggtc gagcgggtgc ccgagacgcc
    11041 gcaacggcag cgcgatctcg cggtggcgta ctcgctacgg ggcgatcacg cgcaggcgca
    11101 ggcgctcctg aagccgctca cggactcgca cagtcgcgcg atcgagggtg gcaccctgaa
    11161 gcggcgctgg ctggagactg gcaagacgat gtggctgcag gcagcgtgcg atgcctacga
    11221 ggccggatat gcgatcgacg gcgatcacta tccgggcatc aacgccgcgt attgtgcgtt
    11281 cgagctcggt cgccggagcg acggcgagcg gatcgcgcgc gagatcatcg cgcgcctgca
    11341 gcccatcgcc acgcgtgaca agtggcaaga ggcttctctc gccgaagccc acctgttgac
    11401 cggcgatctc gaggccgcgc gggtgcgcta tcaggccgcc gcgaagaagc acctggcgca
    11461 gtggcgcaat ctcgcgatca tgagaaggca ggcgcgcaag atcctcgagc tcctgggcca
    11521 gccgagagcc tcgttcgatg ccatcttccc ggtccccgcg accgcggcgt tcaccggaca
    11581 tcgcgtcgac cccgacgaga cgagaggccg gttcgcgccg agccgggtcg acggcgtgaa
    11641 gcgtcgcctg gcacgagccc tcgaagaacg cgatgttcag ttcgggttct cgtccgccgc
    11701 cgacggcggc gatatcctgt tcctcgaggc gctgctcgag cgcggcggag aagcgcacgt
    11761 ctacttgccg ttccccgcgg atgcgtttgc gagcagctcg gtcgcgcagg cctggcaaga
    11821 tcgtttccac gccctcatcc agaagctcgg gcggcgcgtc gtcgtgctgt ccgatcagat
    11881 gccggaatcg ggtccgttgc gcgatgccgg ctttgcgaac tgcaacgctg cgatccagga
    11941 ggccgcgcgg catcacgccg ggaccctgga tgagcccccg ctgctgatcg ccgtcgtggc
    12001 aggggatggg acggatggga cgggcggcgt cggaggggcc cacgatgccg tccggcgctg
    12061 gatcgatcgc ggagaccgcg tgttccagat cgacccgcgc accgtctgat ctccgcacgt
    12121 aggcctgccc tcacgatctc cgcgggcatt gccacgcgag cctggaggcg cctgaacatc
    12181 gctcggcgtg aggtgagtgt gggaatcaaa ccggtgacgc gcatcgtcat cgtcaggcgg
    12241 gtcagcagat cgaactggcc gccgcgcgcg aggatccgcg gcgtgtggtt ttggaggaca
    12301 ctgtgcgcgt gacgaagctc gcgatcgaag gaacatgctt gtgcaaggcc gtggagatcg
    12361 gggtgacgcg gcggccacga caggtgacgc agtgcaactg ctcggtgtgt cgccgctacg
    12421 gcacgctgtg ggcgtactac cggcgatcgg cggtgtcgat caccgcgccg cgcggcgccc
    12481 tcgaggacta ctcggtgcgt gcgggcgggc tgaagttcgt gcggtgccgg tcgtgcggtt
    12541 gcgtcatctg ctgggaccgc ccgggcggag ggcgcgacca gcgcatgggc atcaacacgc
    12601 gcctgctcga ccacgcgcag atggcggacg taccggtcaa ggtgcttgac ggcgaccaga
    12661 gctggcgcgt gctcggcgtg tatgtgaagc ccgagatgtg ggtctcaccg tctcgcgtcg
    12721 agaagcagcc tcactatcag cgaccggcct ccacatcgcg tcgagcgccg cgctcgagac
    12781 cgcgatcacg agcgacggcg cgctgagcca gaccgggagc ggcgatctgg agtcagaaat
    12841 ctgagcggag ccagatctgg agccaagatc tggagtcaga aatctgagac gtcgagatcg
    12901 tagcgaggag tgccgccggc acggcgtcgg tgcggccggt gagccgcccg ctggacccgg
    12961 acgtcgagtc attgctgctc gagctcgacg gtgaatacgc cgaccacctg gtcggcaggt
    13021 gttccgcgat cgagctcgcg gagaggacgt tgctggccag gatcgcccgg tcttgcgagg
    13081 agaacgagct gcagcggcga gaatcgcgga atggagtgat cgggagcgtg agtggatcat
    13141 gagagaccac cgagcgcgag cgatccctca cgatcgttcc atggttgtga cttcgatgcc
    13201 ggtggcgacc ggtggtgtga gttgcgctgc acttccacgg caggccgatg cggaaccaca
    13261 tcatgagtga attttctgtt aatcaaattt ctgcatgacg cggcgatttg tccggcatta
    13321 gtagtgggcc agatcgactg gcatcgaaaa attgccgggt ggatccacga gtggttgccg
    13381 aggcgatgga caaaccgatt tcatgaagta cccctcaccg agccagacgc gaccgcgact
    13441 cggccacgat ccgacgcttc atcgcgatgg ctggcgatct gccgcgaccg tcccatggac
    13501 gttcacgccg gcgcggttcc cgtcgggagc aacgccacga cccgcggtaa ctgcatattc
    13561 agctctcaaa atcaagacag caagcaagaa accgcatgag aggagcagta gatgaaaagt
    13621 agcattcgca gcaaggtacg gcatcgaact gggacgctcg cggtgtgcag cggcgtcctc
    13681 ctcgccgggg ggatcggcgc cgggtgcgca gatgctcccg acgacaggga gccggtagag
    13741 ttcgccagca ccgagcaatc ggtcacggtc gccggcgatc cgttgcccgg gatcaccgca
    13801 acggactttg ccgcggcgaa ggccaacttc gcggccgtgg aggggctccc ggacgggctc
    13861 ggtccgatct tcaacgagaa ggcctgcggc aactgccaca cccagggcgc gatcggtggc
    13921 gccggcgtcc agatcgagcg ccggttcggc cggttcgaca acgggatctt caacagcctc
    13981 tccaacgaag gcggatcgct gcgccagctg ttcacggtgg gcgcgttcac cgggttgggc
    14041 ggccaggcct gcaacccggc gctcgagcac gagccggccg acgcgaccgt gcacaacgtc
    14101 ggccgtctga cgacgccgct gttcggcgcc ggcctgatcg acacgatccc ggactcggtc
    14161 atcatcgcga acgccaacgc gcagccgctc tcggtgcgcg gcaccgtcaa caacgtgaag
    14221 gtcctgctgc cgaaccccgc ggatcccacc caggtgctca acggaacgcg cgtgggcaag
    14281 ttcggctgga aggccggcat tgccgcgctc gctcagttcg ccgcggatgc ctacgtcaac
    14341 gagatgggca tcaccacgca gcactgcatc gggggtacgt cggtgacggc cttcgccaac
    14401 gagtcgaagc ccaacggcat cgcggtgcct gcaggctgcg acgatcaggc tccgccgcac
    14461 gctgccatcg cgggggtccc cgccaatacc gacgatgtgg tgggggcctg tgcgcccggc
    14521 cagtccgagc tccaggacga cgtcgcgaac ttcttgaagt tcatgacgtt cctcgcgccg
    14581 gtgccgcgcc tggcgatcga tccgaacgtc aacctccagg gcggcacggt gttcaacgcg
    14641 atcggctgcg ccgattgcca cctgctgaag gactacaaca cgggtcccaa tccgccgaac
    14701 ggcgtgccgg cgaacatgtt gttccggccg cgcacggact tcctgctcca cgacatcggc
    14761 accggcgaca tgatcggcaa cgatggtgac acgctggcgc gcaccaagat gatgcgcacc
    14821 gcgccgctgt ggggcctcca cgtccgcacg aagttcatgc acgacggcag ccagacgtcg
    14881 atcgtgggcg cgatcaacgc acacggtggc caggccgcca cggcggcatt caacttcttc
    14941 ttcggtatca gcgcctcgga ccagaacgcg atgctgaccg cgatgatgtc ggactgatcg
    15001 gaggagccgc tgcgcggccg tcagccgacc gcgaagccgt cgtagtcgcg cgacgcgacg
    15061 gccgcgcagc gctccgcgta ggccgggaaa ccggccgcga gtggcatgaa cacgcgcggc
    15121 ttgccggcga cgttggcacc gaggtaccag gattggcagt tcgggtacac ggtgcggtcg
    15181 gcggccgcgt tgacgaacgc cacccagcgc gactccgcgt cctcgctggc ctcgatcgtc
    15241 gcgtgaccgc gcgcgtcgag ccacgccacg cagccggcga tccagtcgac gtggtgctcg
    15301 accgcgacga agaagttggt gaacgccgag gtgctgccgg ggccggcgac gtagaacagg
    15361 ttcggaaatc cggccacggt gaggccgagg tagttgaccg ggccggcgcg ccacttgtcc
    15421 tggatgcgca ggccgccgcg gccgcgcaga tcgatgcgca cgaccggccc ggtgaaggcg
    15481 tcgaacccgg tcgcgagcac caggacgtcg aagcggtgct cgcgcccgcc ggccaccagg
    15541 ccgcccggcg tgatcgcctc gatcggctgc gtggcaatgt cgaccagcgc gacattggat
    15601 cggttgtagg tctcgtagta gccggtatcg acggccatgc gcttgcaacc gatcggatag
    15661 cgcggacaca gcagcgccgc cgtggcgggg tcgcggacga tctggcggat cttgcggcgc
    15721 acgaactcgg ccgccagctc gttggccccc gggctggtca acagatcccg gaacgcgccg
    15781 aggaagctga acccgccgat ccgccagcgc tcctcgaaca cggcctcgcg ctgcgcctcg
    15841 gtggcgtcga gcgccgacat cggcgccgcg gacagctcgc cgccgaaccc gctgaacatc
    15901 ttgcggttgc gcgcgcggaa cccggcgtag tcggccttga cccgcgcctc ccacgccgga
    15961 tccagcgggc cgttgcgggc cggcacggca taggcggcgg tgcgctggaa cacggtcagg
    16021 gcgcgcgctt gctcggcgag gatcgggatc agctgcacgc ccgacgagcc ggtgccgatc
    16081 acggcgacgt gccgctcgct caggtcgacc ggcgggtcgg gccaggcggc cgagtgcagc
    16141 accggcccgg cgaagcggtc gaggccgggg aacgcgggca cgttgggcga cgacagcggc
    16201 ccgatggcca ggaccaggaa ccgcgcggtc cacgcggcgc cggcgtcgct caccacccgc
    16261 caccgccgcg cggcctcgtc gaagtgcgcc gccgcgaccc gcgtgccgag ccggatgtcg
    16321 cgccgcagat cgaagcggtc cgcgacgtgg ttggcgtagc gcagcagctc gggctggccg
    16381 gcgtagcgct cggtccagcg ccactgctgt tgcagcggct cggagaacga gtacgagtac
    16441 tcgaggctct ggatgtcgac ccgcgccccc gggtagcggt tggtccacca cgtgccaccc
    16501 acgctcggcg ccgcttccag cacgacggcg gtgagcccga tgccgcgcag cctgtacagc
    16561 gcgtacagcc cgccgaaccc ggcgccgacg atcaggacgt cgaccgcgtc ggccgggctc
    16621 gctcgctccg gcggagcggt gggatcgtgc gccgcggtca tcgccgccat catgacaaac
    16681 cgcggcgcat ccgcctagcc cggcctggga gcgcgagtac gtgtccgatc tgctcgcgcg
    16741 ctgcggcaac ctgagcgatg ccgcgcgcaa ggccggcatc gcgcgcggcc acctccaccg
    16801 gctgctgcgc aagcacgatc tcggctgacg cacgcgcggg cggtgactac cagaggtcga
    16861 agtcggcgcc gaggcaggcc tgcacgatga acgggttgac ccacaggctc atcttcgcgg
    16921 tgccgacctc gacgctgtcg aacagcggca ggccgggggt cttgacgttg acgtgctgga
    16981 cctcggcgcg ggcgagcatg aaggcgatga acttcacgtc gatccgggcg tagatcgatc
    17041 gcgacagccg cgcatcgagc ccgccctgga gcaccaggcc gggcgccgga tcgatcgaca
    17101 tctcggggtg gttgacctcg gtgaggatgg gattggtcac cctggcgttg cgggcgaaca
    17161 gcacgctcgg gccgatgccg agatacggcc agacgtagcg atggtcgatc agccggtagg
    17221 tcgcggtgag cacgacgggc gccgcctgcg cctcgccgag atcggggccg agcggctgga
    17281 tgccggtggg aatcccgagc gccatcggcg cgagcgacgt cgtggcgagc gtcccggtgg
    17341 cgcggaactt gaccgtgaac ggcagaccga gcacgagctc gcccgaccag cggcgactcg
    17401 ccgtccgtgg cacccagccg aggatcgccg cggggatggt cgccgaatcg atggtcgcgc
    17461 ccgagccggc gatcgggccg ttgtggacgg cgagggtcgc cgcgccgtgg acatcggcga
    17521 gctcgagctc gcgcgacgac gagaccggct tgaccagggc accgccgagg cggacgtaga
    17581 acctggggcg gggcggcgac ggtgcgagcg cggacacggc cggcgcttcg gtcaccggcg
    17641 cgggagcgtc gaccaccggc gcgaggccgc cggccgccgg ctcggcgcg
//
LOCUS       DASVOR010000655         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_269149,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000655 DASVOR010000000
VERSION     DASVOR010000655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269149"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VF516_12975"
     CDS             complement(<1..636)
                     /locus_tag="VF516_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108637.1"
                     /translation="MGLLACHDKEPPPPQDGVVLIQPGAPPLAPLRYQLTRGATVQSR
                     LVSDVDVQSPELRARMPSQVIDLETVVEDVLASGDARLRITVTGADLRDRPGAASTDV
                     VRAQTAALRGVVITETVAPDGKISDARALAGASPDKLHRELDVLLQTLARVATRLPAE
                     PVGIGATWRERRTLPEGGIRAVSEITYTLTARTGTTIAFTTAAQASGPPQTV"
     gene            1030..1500
                     /gene="bfr"
                     /locus_tag="VF516_12980"
     CDS             1030..1500
                     /gene="bfr"
                     /locus_tag="VF516_12980"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEX8108638.1"
                     /translation="MKGNDEVLTLLNNLLTNELTAINQYFVHAKMCENWGFERLAHKI
                     REESIDEMRHADQVISRILFLEGVPNLQRYHKLRVGETVKEQLESDLQLEYAAIQFLN
                     QGIEVTRKLGDNATEDLFTRILVGEEQHTDWLETQLELIRQVGEQNYLAQQMKK"
BASE COUNT          250 a          503 c          532 g          219 t
ORIGIN      
        1 cacggtctgc ggcgggccag aggcctgggc cgcggtggtg aaagcgatcg tcgtgccggt
       61 gcgcgctgtg agcgtgtagg tgatctcgga cactgcgcgg atcccgccct cgggcagcgt
      121 ccgccgctcg cgccaggtcg caccgatgcc gaccggctcg gccggtagcc gcgtcgccac
      181 gcgcgccagg gtctgcagca ggacgtcgag ctcgcggtgg agcttgtcgg gcgaggcgcc
      241 ggcaagggcc cgcgcgtcgg agatcttgcc gtcgggcgcc acggtctcgg tgatcacgac
      301 gccgcgcagc gccgcggtct gggcccgcac cacgtcggtc gacgccgcgc ccggccggtc
      361 gcggaggtcg gcgccggtca cggtgatccg cagccgggcg tcgccgctgg cgagcacgtc
      421 ctcgaccacg gtctccaggt cgatgacctg cgacggcatc cgcgcgcgga gctcggggct
      481 ctggacgtcg acgtcgctga ccagccggct ctggaccgtc gcgccgcggg tcagctggta
      541 gcgcagcggc gcaagcggcg gagcgcccgg ctggatcagc accacgccgt cttgcggcgg
      601 cgggggctcc ttgtcgtggc aggccaagag ccccaccagc gcaaggtacg tgcagcgcat
      661 cgccgggagt gtgtcgcaaa tagctgtgat ccaccagaaa ttttcgctgc tcggcggggc
      721 cctgaggcgc cttgacagaa ctggatttga aatgcattat caatctcaga gcctgtgctg
      781 gtctgcatct gtcaccccac ctcggagcgc gagctcgacg ccgtcatcga tgatggagcg
      841 cgcaccgtcc aggagatcgg ccagcgctgc ggcgccggga ccggctgcgg gtcgtgcgtc
      901 gaggagctgc gcgatcgcct ggtcagccga ggggcgaatg gttgcccgcg cgattgtgcg
      961 ggcgatctcg tgtcgttaag gtcagctcgc tagtccaccg cgccaggcac ggcgcagcga
     1021 ggcagtccga tgaagggcaa cgacgaggtg ctcacgctcc tcaacaacct gctcaccaac
     1081 gagctgacgg cgatcaacca gtacttcgtc cacgccaaga tgtgcgagaa ctggggcttc
     1141 gagcgcctcg cccacaagat ccgcgaggag tcgatcgacg agatgcgcca cgcggatcag
     1201 gtgatcagcc ggatcctgtt cctcgagggc gtgcccaacc tgcagcgcta ccacaagctg
     1261 cgcgtcggcg agaccgtcaa ggagcagctc gagagcgacc tccagctcga gtacgccgcg
     1321 atccagttcc tgaaccaggg catcgaggtc acgcgcaagc tgggcgacaa cgcgaccgag
     1381 gacctgttca cccggatcct ggtcggcgag gagcagcaca ccgactggct ggagacccag
     1441 ctcgagctga tccgccaggt cggcgagcag aactacctcg cgcagcagat gaagaagtga
     1501 cacg
//
LOCUS       DASVOR010000656         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_152409,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000656 DASVOR010000000
VERSION     DASVOR010000656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152409"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /gene="lipB"
                     /locus_tag="VF516_12985"
     CDS             <1..520
                     /gene="lipB"
                     /locus_tag="VF516_12985"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX8108639.1"
                     /translation="GRRRDAEANVLMPGDVEVIGIERGGDVTYHGPGQIVAYPIVLLG
                     DGERDLHRYLRNLEEGVIATCARFGLACDREPGKTGVWLTTPTGPAAGMRRKICSMGI
                     ACRKWVTFHGLALNVTSDLGYFRRINPCGFEASVMTSMAEQLGGVVELAAVRNSLAEE
                     LGRALGRTTRPS"
     gene            complement(517..1209)
                     /locus_tag="VF516_12990"
     CDS             complement(517..1209)
                     /locus_tag="VF516_12990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108640.1"
                     /translation="MSSDLRAFVAEEIRLHPRVAYQGLLTEEGAATRVAAALPALQRF
                     RHDYAGAISVVDWDHRLPSQNLVLRIYGYYGEDTLDAGYEAFDDRLDQIAERDKYPEF
                     DVPDFDGLAADEAYEIELSPTGQIGRCRLTSAWRRTVASHDAAAAVALVQACEEYGKL
                     VAASPSRPAYLGDLEAVSWTPPCETDHERWTLDVWYLLAFDGRLGSGRSFLADLEARQ
                     IVSVRDFSVRTG"
     gene            complement(1276..1968)
                     /gene="hisG"
                     /locus_tag="VF516_12995"
     CDS             complement(1276..1968)
                     /gene="hisG"
                     /locus_tag="VF516_12995"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEX8108641.1"
                     /translation="MTRPIILALPKGRILEEAAAVFRRAGFDLSPVLGDSRRLVHDCG
                     PLRVLVLRSSDVATYVTHGAADVGVAGSDVLDEEGRDLFEPLDLAIGRCRMIVAERAE
                     PAPPAPGHLPASRTWPTSRAWDDAADRAQAHLRIATKYPRTTRAYLQRKGITAEVIKL
                     SGAIELGPLTGLCDRIVDITQTGETLRQNGLVEIDTVAQVSSRLVVNPARLKLDGDRL
                     AALIDALERAVS"
     gene            complement(1992..>2804)
                     /locus_tag="VF516_13000"
     CDS             complement(1992..>2804)
                     /locus_tag="VF516_13000"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEX8108642.1"
                     /translation="ARRLKGATIVLDVPTVTGCENLIMAAALAKGRTVIENAAREPEV
                     EELALVLNKMGARIQGAGTAIITIEGVDELQPVEHAIIPDRIEAGTFAVAAAMTRGDV
                     LLDGARPEHLDAVIAKLRTAGVQVAAEGGGLRVRGTGEIQPVDIVTQPHPGFPTDMQA
                     QFMVLACLARGQSVIKEMIFENRFMHVPELGRMGADIQIAGRVAMVRGGSRLTGAQVM
                     ATDLRASASLILAGLVAAGKTEVLRVYHLDRGYAAIEAKLRAVGAKIRRARQ"
BASE COUNT          382 a          975 c         1044 g          403 t
ORIGIN      
        1 cggccggcgg cgcgacgccg aggccaacgt gctgatgccg ggcgacgtcg aggtgatcgg
       61 catcgagcgc ggcggcgacg tgacctacca cggcccgggc cagatcgtcg cctacccgat
      121 cgtcttgctc ggcgacggcg agcgcgacct gcaccgctac ctgcgcaacc tcgaggaggg
      181 cgtgatcgcg acctgcgcgc ggttcggcct ggcctgcgat cgcgagcccg gcaagaccgg
      241 ggtgtggctc acgacgccca ccggccccgc tgctgggatg cggcgcaaga tctgctcgat
      301 gggcatcgcg tgccgcaagt gggtcacctt ccacggcctg gcgctcaatg tcacgtcgga
      361 cctcggctac ttccggcgga tcaacccgtg cgggttcgag gcgtcggtga tgacgtcgat
      421 ggccgagcag ctcggcggcg tggtggagct ggccgcggtg cgcaactcgc tcgccgagga
      481 gctcggccgg gcgctcggca ggaccacccg gcccagctaa ccggtgcgga ccgagaagtc
      541 gcggaccgag acgatctggc gggcctcgag gtctgccagg aagctgcggc ccgagccgag
      601 ccggccgtcg aacgcgagca ggtaccagac gtcgagcgtc cagcgctcgt ggtcggtctc
      661 gcacggcggc gtccacgaca cggcctccag gtcgccgagg taggccgggc gcgacggcga
      721 ggccgcgacc agctttccgt actcctcgca ggcctggacc agggcgaccg ccgcggcggc
      781 gtcgtgcgac gcgacggtgc ggcgccacgc cgaggtcagg cggcaccgcc cgatctggcc
      841 ggtcggcgac agctcgatct cgtaggcttc atcggcggcc agcccgtcga agtccgggac
      901 gtcgaactcc gggtacttgt cgcgctcggc gatctggtcg agccggtcgt cgaacgcctc
      961 gtagccggcg tcgagcgtgt cctcgccgta gtaaccgtag atccggagca cgaggttctg
     1021 cgacggtagc cggtggtccc agtcgaccac cgagatcgca ccggcgtagt cgtggcggaa
     1081 tcgctgcagc gccggcaatg cggccgccac gcgggtcgcg gcgccttctt cggtcaagag
     1141 gccctggtag gcgacccggg ggtgcagccg gatctcttcg gcgacgaagg ctcgtaggtc
     1201 ggacgacacc tcggggccga ttgtacacgt ggatcccggg cggcgctcgg ggtgacattg
     1261 catcccctgg cggtgtcagg aaactgcgcg ttcgagcgcg tcgatcagcg ccgccagccg
     1321 gtcgccgtcg agcttgagcc gcgcagggtt gaccaccagg cgcgatgaga cctgcgccac
     1381 ggtgtcgatc tcgaccaggc cattctggcg cagggtctcc ccggtctggg tgatgtcgac
     1441 gatgcgatcg cacagcccgg tcagcggccc gagctcgatc gctccggaca gcttgatcac
     1501 ctcggcggtg atgcccttgc gctgcaggta ggctcgcgtg gtccgggggt acttggtggc
     1561 gatccgcagg tgggcctggg cgcggtcggc cgcgtcgtcc caggcccgag aggttggcca
     1621 ggtccgagag gcagggaggt ggcccggagc gggcggcgcc ggctcggcgc gctcggcgac
     1681 gatcatccga caccggccga tcgcgaggtc gagcggctcg aacaggtcgc ggccctcctc
     1741 gtcgagcacg tcgctgccgg cgacgccgac gtcggccgcg ccgtgggtga cgtaggtcgc
     1801 gacgtcggac gaccgcagca ccaggacgcg cagcgggccg cagtcgtgga ccagccggcg
     1861 cgagtcgccg agcaccggcg acaggtcgaa gccggcgcgg cggaacaccg cggcggcttc
     1921 ctcgaggatg cggcccttgg gcagggcgag gatgatcggt cgggtcatgg gctttggggt
     1981 agccggcggc ctcactgcct ggcgcgccgg atcttcgcac caaccgcgcg cagcttcgcc
     2041 tcgatcgcgg cataaccgcg atcgaggtgg tagacccgca ggacctcggt cttgccggcc
     2101 gcgaccaggc ccgcgaggat caggctcgcc gaggcccgca ggtcggtcgc catgacctgc
     2161 gccccggtca gccggctgcc gccgcgaacc atcgcgaccc gcccggcgat ctggatgtcg
     2221 gcgcccatcc ggccgagctc ggggacgtgc atgaagcggt tctcgaagat catctccttg
     2281 atcaccgact ggcccctggc caggcaggcg agcaccatga actgggcctg catgtcggtc
     2341 gggaacccgg ggtgcggctg ggtgacgatg tcgaccggct ggatctcgcc ggtgccgcgc
     2401 acccgcaggc cgccgccctc ggccgcgacc tggacgccgg cggtgcgcag cttggcgatc
     2461 accgcgtcga ggtgctcggg gcgagcgccg tcgagcagga cgtcgccgcg ggtcatcgcg
     2521 gccgcgaccg cgaacgtgcc ggcctcgatc cggtccggga tgatcgcgtg ctcgaccggc
     2581 tggagctcgt cgacgccctc gatcgtgatg atcgctgtgc cggcgccctg gatccgcgcc
     2641 cccatcttgt tgagcaccag cgcgagctcc tcgacctcgg gctcgcgggc cgcgttctcg
     2701 atcacggtgc ggcccttcgc gagcgcggcg gccatgatca gattctcgca gccggtcacg
     2761 gtcgggacgt cgagcacgat cgtcgcgccc ttgagccgcc gggc
//
LOCUS       DASVOR010000657         2995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_152428,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000657 DASVOR010000000
VERSION     DASVOR010000657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2995
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152428"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..834)
                     /locus_tag="VF516_13005"
     CDS             complement(<1..834)
                     /locus_tag="VF516_13005"
                     /inference="COORDINATES: protein motif:HMM:NF015376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HEX8108643.1"
                     /translation="MATRCLVLAVVLAGCAELGVVGDGTSISVGKASNGYLIEGARLP
                     DHGEGFTTRDVWRARDNRYGTDELIDLVVGVARRMHRQVAGVQIVIADLSGRGGGERY
                     AFHRSHQSGRDADLLYYLRDAGGQPFEPDAMHVFDAAGRARDGSGLTVDVPRTWLLVK
                     ELITAPEAPVQWIFLYEPLARRLLDHAARIGEPEAVIGRARKTLKQPGDSARHDDHMH
                     VRVYCSPADRAYGCIDLGPMELLAERQAEPSPVSELMAALTAPPVPPPATLPPSPPPT
                     GD"
     gene            complement(992..2917)
                     /gene="dnaK"
                     /locus_tag="VF516_13010"
     CDS             complement(992..2917)
                     /gene="dnaK"
                     /locus_tag="VF516_13010"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEX8108644.1"
                     /translation="MGKIIGIDLGTTNSVVAIMEGKDPKVISNEEGARLTPSVVAFDD
                     KGDVLVGQIARRQAITNPENTVFSSKRFMGRKFSDPITQEESKRVPFKIVAAPNGDAA
                     FEIRGKRYSPPEIGARVLMKLKRAAEEYLGEPVTEAVITVPAYFNDSQRQATKDAGKI
                     AGLDVKRIINEPTAAALAYGLDKTKEQLIAVFDMGGGTFDISVLEVGDKVVEVVSTNG
                     DTHLGGDDIDNRLMDWLVAEFKKDTGIDVSKDKMVLQRLKEAAEKAKIELSSVQETEI
                     NLPYLTADATGPKHLLKKLSRAKFEQMIEDIVARAIEPCKKALADAGKKVSDIHEVVL
                     VGGSTRIPMVQAKVKEFFGREPNRSVNADEVVALGAAVQGGVLSGDVKDILLLDVTPL
                     SLAIETLGGVATVLIGRNTTIPTRKSETFSTAADNQTSVEVHVTQGERPMAKDNKTLG
                     KFQLTGIPPAPRGVPQIEVTFDIDANGILNVAAKDKATGAERTIRIEASSGLSESDIK
                     RAVEDAAQHEAEDKLRKETIEARNQLDTLVYSTRKLVQENSAKLGDPEKLMIEEELKE
                     AEAVLERNKDADRPDELRAAFEKLQGAAHKLAEAMYKSAGGGQPGGDGAPSGGGGGGQ
                     QGGGKDDVIDAEFEDKQ"
BASE COUNT          379 a         1046 c         1040 g          530 t
ORIGIN      
        1 atcgccggtc ggcggcgggc tcggcggcag ggtcgccggc gggggtaccg gcggcgcggt
       61 cagcgcggcc atcagctcgc tcaccggcga cggctcggcc tggcgctcgg cgagcagctc
      121 catcggtccg aggtcgatgc agccgtaggc ccggtcggcc ggcgagcagt acacccgcac
      181 gtgcatgtgg tcgtcatggc gcgcgctgtc gcccggctgc ttgagggtct tgcgcgcgcg
      241 gccgatcacc gcctcgggct cgccgatccg cgcggcgtgg tcgagcagcc ggcgcgccag
      301 cggctcgtag aggaagatcc actggaccgg cgcctcggga gcggtgatca gctccttgac
      361 cagcagccag gtccgcggga cgtcgacggt gagcccgctg ccgtcgcgcg cccgcccggc
      421 cgcgtcgaac acgtgcatcg cgtcgggctc gaacggctgg ccgcccgcgt cgcgcaggta
      481 gtacaggaga tcggcgtcgc gcccgctctg gtgcgagcga tggaacgcgt agcgctcgcc
      541 gccgccgcgg ccggacagat cggcgatcac gatctggacg cccgccacct gccggtgcat
      601 ccgccgcgcc acgccgacca cgaggtcgat cagctcgtcg gtgccgtagc ggttgtcgcg
      661 cgcgcgccag acgtcgcggg tggtgaagcc ctcgccgtga tcgggcagcc gcgcgccctc
      721 gatcaggtac ccgttgctcg ccttgccgac cgagatgctg gtgccgtcgc cgaccacgcc
      781 gagctcggcg cagccggcga gcaccaccgc gagcacaagg caccgcgtcg ccatcgagtc
      841 cgtccagtat ggatcgcgcc cgccccgcgg cgatcaaaaa acgcgacgac accggcggga
      901 tccgccggtg tcgccggaac acacacccca cgggccgccg ggggtggccg gaggcccgag
      961 ggaggtcgag gggccgaggg gccgagggga gctactgctt gtcctcgaac tcggcgtcga
     1021 tcacgtcgtc cttgccgccg ccctgctgcc cgcccccacc gccgccggac ggcgcgccgt
     1081 ccccgccggg ctggccaccg cccgcggact tgtacatcgc ctcggcgagc ttgtgggccg
     1141 ccccctgcag cttctcgaac gccgcgcgca gctcgtccgg ccggtcggcg tccttgttgc
     1201 gctccaggac cgcctcggcc tccttgagct cctcctcgat catcagcttc tcggggtcgc
     1261 cgagcttggc gctgttctcc tggaccagct tgcgcgtgct gtagaccagc gtgtcgagct
     1321 ggttgcgggc ctcgatggtc tccttgcgga gcttgtcctc ggcctcgtgc tgcgccgcgt
     1381 cctcgaccgc gcgcttgatg tcggactcgg acagaccgct cgacgcctcg atccggatcg
     1441 tgcgctcggc gccggtcgcc ttgtccttgg cggcgacgtt gaggatgccg ttggcgtcga
     1501 tgtcgaacgt gacctcgatc tggggcacgc cgcgcggcgc cggcgggatg ccggtcagct
     1561 ggaacttgcc gagcgtcttg ttgtccttgg ccatcgggcg ctcgccctgg gtcacgtgga
     1621 cctcgaccga ggtctggttg tcggccgcgg tcgagaacgt ctcgctcttg cgggtcggga
     1681 tcgtggtgtt gcgcccgatc aggacggtgg cgacgccgcc gagcgtctcg atcgccagcg
     1741 acagcggcgt gacgtcgagc agcaggatgt ccttgacgtc gcccgacagc acgccgccct
     1801 ggaccgccgc gccgagcgcg acgacctcgt cggcgttgac gctccggttg ggctcgcggc
     1861 cgaagaactc cttgaccttg gcctggacca tcgggatgcg ggtcgagccg ccgaccagga
     1921 cgacctcgtg gatgtcggac accttcttgc cggcgtcggc gagcgccttc ttgcacggct
     1981 cgatcgcgcg ggcgacgatg tcctcgatca tctgctcgaa cttggcgcgg ctgagcttct
     2041 tgagcaggtg cttgggcccg gtcgcgtccg ccgtgaggta cggcaggttg atctcggtct
     2101 cctgcacgct cgacagctcg atcttggcct tctccgcggc ctccttgagc cgctggagca
     2161 ccatcttgtc cttgctgacg tcgatgccgg tgtccttctt gaactcggcg accagccagt
     2221 ccatcagccg gttgtcgatg tcgtcaccgc cgaggtgggt gtcgccgttg gtcgacacga
     2281 cctcgacgac cttgtcgccg acctcgagga cggagatatc gaacgtgccg ccgcccatgt
     2341 cgaacacggc gatcagctgc tccttggtct tgtccaggcc gtaggccagc gcggccgcgg
     2401 tcggctcgtt gatgatgcgc ttgacgtcga gcccggcgat cttgccggcg tccttggtcg
     2461 cctggcgctg gctgtcgttg aagtacgccg ggacggtgat caccgcctcg gtgaccggct
     2521 cgccgaggta ctcctcggcg gcgcgcttga gcttcatcag cacgcgcgcg ccgatctcgg
     2581 gcggcgagta gcgcttgccg cggatctcga acgccgcgtc gccgttgggg gccgcgacga
     2641 tcttgaacgg aacgcgcttg gactcctcct gcgtgatggg atcgctgaac ttgcgcccca
     2701 tgaaccgctt gctggagaac acggtgttct ccgggttcgt gatggcctga cgccgggcga
     2761 tctggcctac gaggacgtcg cccttgtcgt cgaaggcaac caccgagggg gtcaggcgcg
     2821 cgccttcctc gttcgagatg accttggggt ccttgccttc catgatcgcg accacggaat
     2881 tcgtggttcc caggtcgatg ccgatgatct tgcccatgac gtgaattcct cgattcttgg
     2941 atgtgtgtgc tgcgcaacct aagtagcgtc caagcgaccg tcaagacagg cacgt
//
LOCUS       DASVOR010000658         5228 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_356990,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000658 DASVOR010000000
VERSION     DASVOR010000658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5228
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_356990"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2104
                     /locus_tag="VF516_13015"
     CDS             <1..2104
                     /locus_tag="VF516_13015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108645.1"
                     /translation="GKATDVKLTSASTNIATVPDHVTVSAGASSAEIQYTSVAPGTAI
                     FHATAGSDSVTTTAAVVDAMRVTPPSATTLEVGAHGILLEQLNITVPDAITVPLSSSQ
                     TSIATVDDSVAISKFSSSGLAQITAVSPGASIISASFSGQQASQTITVVDKAQISQVS
                     GTSLFEVGGHGTAQISLNAQLASAAQLTVSPSDASVVSVARTFTIAPGSTFASLDVQG
                     MATGSTNMVFSLNNSTATLPIMVVSKAQLENVFVNTAPSVGYTMQLTVSMNVIAATAH
                     DVTLTSSDPTVVAVPPTVTVFAGKSSASVAITPLKAGDAVITASYNGISRQIDIPIGN
                     SGYAINMYGQTRYVVGALGSISVQTGVSAPTTVSLQSSDPTVVTVPSSLIVNNSEVAP
                     LTALKAGTATITATTNGASATYQVAVVASPGISQFGPSFSVPVNGSMNGYMYLDTPSP
                     SGTAITFTTSDATIAPAPASVTLTNGQTSIPLLIKGGATGTATITANIAGARASAVVY
                     VGSSSAGTGFQNLYVNGYSNLEIGAATLLYAYFYPSPPTGATGTITFGTAGILSTPST
                     TLPISSSSCCNYFPVVGAVAGSSDVTVTVNGVSQTTTINVVSSPTYSLGLTSTVKTGS
                     VVTATISSNAVVAADRTFTLSSSNPAVATVTSPTATIGPSSSSLQATFGVRGVAAGTA
                     TITGTISGKTVTANITVQ"
     gene            complement(2142..2957)
                     /locus_tag="VF516_13020"
     CDS             complement(2142..2957)
                     /locus_tag="VF516_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108646.1"
                     /translation="MLFRVSTRAAVLSGLATWSGCEGCSGGGFDAGGPDLEAAVGRFS
                     LSWSVIDQTTGQPVSCDQLDPNATVFVEAVRDGTGAVESFACRNLQGTSMTPLFQGTY
                     SFSYELHVGGATIAKASGPSGIVIGPGQEVQLASITFQVDASGRLELALRAGAAGNCT
                     GGAAITGFSLSLQHAGVPGDTGCVPVVFVLSGGGTYNAGDCSAPAVSRCIAADETLTV
                     ASRPAGSYQIHVRGKKGALDCWVNDDMLRVPPQSALLSQTLNLALQSETPGCQ"
     gene            complement(3072..3620)
                     /locus_tag="VF516_13025"
     CDS             complement(3072..3620)
                     /locus_tag="VF516_13025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8108647.1"
                     /translation="MNTSIATSVRRARSSDTAALTALVNRAFAVERAFLDGDRTTTEE
                     IASLIASGCFLVLEYAGGIGAAVLVQGPGQHPGAPPSHAYLGMLSVQPELQGMGLGVR
                     LVRVAEAMAEAAGATSMWLRIINLREELSRWYKSLGYRAVGTTPFTHHSLKRPAHFID
                     MAKPLTPSACYPVGDAGAAGMA"
     gene            complement(3721..3843)
                     /locus_tag="VF516_13030"
     CDS             complement(3721..3843)
                     /locus_tag="VF516_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108648.1"
                     /translation="MVKAVSNVTVGTVIVLVHVALAERHRRLQPVVPPDRRIVN"
     gene            complement(4379..>5228)
                     /locus_tag="VF516_13035"
     CDS             complement(4379..>5228)
                     /locus_tag="VF516_13035"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HEX8108649.1"
                     /translation="VFAGYRDPAAEVGRLFRKVDGERPTIEVTTPDQTVHRVWRVQSA
                     ELIGALRAQFAPKKLSVLDGHDRYEALLAYRDELAAKQPLALYSSANYALACLVNLGD
                     PTLIAVPRHRVIRGAVASQAALETARKYFVIDRLAGAAGNLGKLRAALDDTIAHQPAF
                     VVVWPGEPDAWKLTLSPDISPVAEGVQVHRALQKLDPIVADLLFLDRAMPGAQREFVV
                     SAEAALAARPEPGGAVVIMRPLTVEQISHVVELGQVLPAGSTAFHPPLAHGLVSAVID
                     PDEDLV"
BASE COUNT          821 a         1909 c         1750 g          748 t
ORIGIN      
        1 cgggaaggcg accgacgtga agctgacatc ggcgtcgacg aacatcgcga cggtgcccga
       61 ccacgtcacc gtgtccgccg gcgcatcctc cgcggagatc cagtacacgt cggtggcgcc
      121 gggcactgcg atcttccacg cgaccgccgg gagtgactcg gtgaccacga cggccgcggt
      181 cgtcgatgcc atgcgggtca cgccgccgag cgccacgacg ctcgaggtcg gcgcacacgg
      241 catcctgctc gagcagctca acatcaccgt gcccgacgcg atcacggtgc cgctgtcgag
      301 ctcgcagacc tcgatcgcca ccgtcgacga ctcggtcgcg atctccaagt tctccagctc
      361 cggactcgcg cagatcaccg cggtcagccc gggcgcatcg atcatctccg cgagcttcag
      421 cgggcagcag gcatcgcaga ccatcaccgt cgtcgacaag gcgcagatca gccaggtgtc
      481 gggcaccagc ctgttcgagg ttgggggcca cggcaccgcg cagatctcgc tcaacgccca
      541 gctcgcgagc gcggcgcagc tcacggtctc accgagcgac gcgtcggtgg tcagcgtggc
      601 gaggaccttc acgatcgcgc cgggctccac cttcgccagc ctcgatgtcc agggcatggc
      661 gaccggctcg accaacatgg tgttctcgct gaacaactcc accgccacgc tgccgatcat
      721 ggtcgtgagc aaggcgcagc tggagaacgt gttcgtcaac accgcgccct cggtcggcta
      781 caccatgcag ctgacggtct cgatgaacgt catcgcggcg accgcgcacg acgtgacgct
      841 gacctcgagc gacccgaccg tggtcgcggt gccgccgacg gtcaccgtgt tcgccggcaa
      901 gtcgagcgcg agcgtggcga tcacgccgct caaggccggc gacgcggtca tcacggcgag
      961 ctacaacggg atctcgcgcc agatcgacat cccgatcggg aactcgggct acgcgatcaa
     1021 catgtacggt cagacccgct acgtcgtcgg cgcgctcggc tcgatctcgg tccagaccgg
     1081 cgtgtcggcg ccgaccacgg tcagcctgca gtcgagcgac ccgacggtgg tgaccgtgcc
     1141 gtcttcgctg atcgtgaaca actccgaggt cgcgccgctc accgccctga aggccgggac
     1201 cgcgacgatc accgcgacca cgaatggcgc gagcgccacc taccaggtcg cggtcgtggc
     1261 gtcgccggga atctcgcagt tcggtccgag cttctcggtg ccggtcaacg gatcgatgaa
     1321 cgggtacatg tatctcgaca cgccgtcgcc gagcggcacc gccatcacgt tcacgacgag
     1381 cgatgccacg atcgcgccgg caccggcgag cgtcacgctc accaacggcc agacctccat
     1441 cccgctgctg atcaagggcg gcgccaccgg caccgcgacg atcacggcga acatcgccgg
     1501 agcgcgcgcc agcgcggtgg tctacgtcgg tagctccagt gcgggcaccg gcttccagaa
     1561 cctctacgtc aacggctatt ccaacctcga gatcggcgcc gccaccttgc tgtacgcgta
     1621 cttctatccg tccccgccca ccggggcgac cggtacgatc acgttcggca ccgccggcat
     1681 cctcagcacg ccgtcgacca cgctcccgat ctcgtcgagc agctgctgca actacttccc
     1741 ggtcgtcggg gcggtcgccg ggtcgagcga cgtgacggtc acggtgaacg gcgtctcgca
     1801 gaccacgacg atcaacgtcg tgtcgtcgcc gacctactcg ctcggattga cgagcaccgt
     1861 caagaccggc tccgtggtga cggcgacgat ctcgtccaac gcggtggtcg cggccgaccg
     1921 gacgttcacg ctgtcgtcga gcaaccccgc ggtcgcgacc gtgacgtcgc cgaccgcaac
     1981 aatcggtccc agcagcagca gcctgcaggc cacgttcggc gtgcgcggcg tggccgcggg
     2041 cacggcgacg atcacgggga cgatcagcgg caagaccgtc accgcgaaca tcacggtgca
     2101 gtagcccgcg cccgccgggg tccgggcgcc cgcggccccg gtcactggca gccgggcgtc
     2161 tcgctctgca gcgccaggtt gagcgtctgg ctcagcagcg cgctctgcgg cggcacgcgc
     2221 agcatgtcgt cgttgaccca gcagtcgagc gcccccttct tgccccggac gtgaatctgg
     2281 tacgagccgg ccggccggct cgccacggtg agcgtctcgt ccgcggcgat gcaccggctg
     2341 accgcgggcg cgctgcagtc gccggcgttg tacgtgccgc cgcccgacag cacgaacacc
     2401 accggcacgc acccggtgtc gcccggaacg ccggcgtgct gcaggctgag cgagaacccg
     2461 gtgatcgcgg ccccgccggt gcagttcccc gccgcaccgg cgcgcagcgc gagctccaag
     2521 cggccgctcg cgtcgacctg gaacgtgatc gacgcgagct ggacctcctg gccggggccg
     2581 atcacgatcc cgctcggccc gctcgccttc gcgatcgtgg cgccgccgac gtgtagctcg
     2641 tagctgaagc tgtacgtgcc ctggaacagc ggcgtcatcg acgtgccctg gaggttccgg
     2701 cacgcgaacg actccaccgc gccggtgccg tcgcgcaccg cctcgacgaa caccgtcgcg
     2761 ttgggatcga gctgatcgca gctcaccggc tggccggtcg tctgatcgat cacggaccag
     2821 ctgagcgaga atcggcccac cgcggcctcg agatccggcc cgccggcgtc gaagccgccg
     2881 ccgctgcagc cctcgcaacc gctccaggtg gcgaggccgc tcagcaccgc ggcccgtgtc
     2941 gacacccgga acaacatctg tccgatccta ccctgggacc gcctcgtgcg acgaagctcg
     3001 tgctcggcga cgcttgccgg ggtgacggtg accgtgacgg tgaccgtgac cgtgaccacg
     3061 cgtacgctag ctcacgccat gccggccgcg ccggcgtctc ccaccgggta gcaggccgac
     3121 ggcgtcaacg gcttggccat gtcgatgaaa tgcgccggac gcttgaggga gtggtgggtg
     3181 aatggggtag ttcccaccgc gcggtagccc aggctcttgt accagcggct cagctcctct
     3241 cggagattga tgatccggag ccacatcgac gtcgcgcccg cggcctcggc cattgcttcg
     3301 gccacgcgca ccagacgcac gccgagcccc atcccttgca gctcgggctg caccgacagc
     3361 atgccgaggt aggcgtgcga gggcggcgcg cccgggtgct gtccggggcc ctggaccagc
     3421 acggcggcgc cgatcccgcc ggcgtactcc agcaccagga agcagcccga cgcgatcagc
     3481 gacgcgatct cctccgtggt ggtgcggtcg ccgtcgagga aggctcgctc gacggcgaac
     3541 gcgcgattga ccagcgcggt gagcgcggcg gtgtcggagg atcgtgcacg ccggacggag
     3601 gtcgcgatcg aggtgttcac accgatcgcg tatcagatcc ctccgacagt cgcgccgaac
     3661 cgcgccgaac cgcgccgggc cgacgccggg ccgacgccgg gccgacgaca gaccccccgg
     3721 tcagttgacg atgcggcgat ccggcgggac caccggctgc aggcggcggt ggcgctcggc
     3781 cagcgcgaca tgcaccagca cgatcaccgt acccacggtg acgttggata ccgccttgac
     3841 cattcgatca gcccagctcc acatgaggtc gctccttgta gtctgtgtcg ggtccgagat
     3901 ccaggggcgt ggccctcctt gcacgctccg tcgggtcgca tcggcgatga acatccgccg
     3961 ctccgtcgat cgatcactct tgacccatgg ccggtccggg cctggaacct atccctcgtc
     4021 tcgctacgaa cagcgtacgc gttgcgcgtg gcaaacgaac gacgacgcag gcacgttgcc
     4081 acggcaactc gatcacgaca acccgcatcg ccgcgcgctg aacggtttct gcgtgagagc
     4141 tcgcataccc gatgagcagc gatgttcgat gtcatgcgat cgatggcgga ttcggtgcgc
     4201 ccccgcagcc atcgcagcac attcgtcgcg ggagcgcgtc gcgcgaggcg atcaccgcgc
     4261 cgagcgtgtc gtcagcgcac agcctcctgg ggcgcgatcg ccgtccacat caccggtgcg
     4321 gtcggtaagc cagctgtcac cgatcgcgag gtgagtcaat ccgccgcgcg ccggcggctc
     4381 acaccagatc ttcgtcggga tcgatcaccg ccgagaccag gccgtgcgcg agcggcggat
     4441 ggaacgcggt cgatcccgcg ggcaggacct gaccgagctc gacgacgtgg ctgatctgct
     4501 cgaccgtcag cggccgcatg atcaccaccg cgcccccggg ctccggcctg gcggcgagcg
     4561 cggcctcggc gctgaccacg aactcgcgct gcgcgcccgg catggcgcga tcgaggaaca
     4621 gcaggtcggc gacgatcggg tcgagcttct gcagcgcgcg gtggacctgg acgccctcgg
     4681 cgaccgggct gatgtcgggc gacagcgtca gcttccaggc gtcgggctcg ccgggccaca
     4741 ccacgacgaa cgcgggctga tgcgcgatcg tgtcgtcgag cgcggcgcgc agcttgccga
     4801 gattgccggc ggcgcccgcc agccggtcga tcacgaagta cttgcgcgcc gtctccagcg
     4861 cggcctggct ggcgaccgcg ccgcggatca cccggtggcg cggcaccgcg atcagcgtcg
     4921 gatcgccgag gttgaccagg cacgccagcg cgtagttcgc cgacgagtac agcgcgagcg
     4981 gctgcttggc ggcgagctcg tcgcggtagg ccagcagcgc ctcgtagcgg tcgtggccgt
     5041 cgagcacgct cagcttcttg ggcgcgaact gggcgcgcag cgcgccgatc agctcggcgc
     5101 tctggacccg ccacacccgg tgcacggtct ggtcgggggt cgtgacctcg atcgtcggcc
     5161 gctcgccgtc gaccttgcgg aacagccgcc cgacctcggc ggcggggtcg cggtagccgg
     5221 cgaacacc
//
LOCUS       DASVOR010000659         4027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_298668,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000659 DASVOR010000000
VERSION     DASVOR010000659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298668"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF516_13040"
     CDS             complement(<1..822)
                     /locus_tag="VF516_13040"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX8108650.1"
                     /translation="MQRSPVQPSPRLPRRRRAATLAAALAVIAPASIAAADDVQPFAV
                     ALPTVTGPIPSTPDNFGFAIEGFDVQPPVPDGYVIEEFFVSGVGNLYEFTPTGIRVVS
                     PCPAAATSGCTSIPYTTRMIVKRPKRDHDFSGTVVIEALNPSGGFDIAAVWDRSRELF
                     TANGDIFIGWSSKSVIINALKQWNPARYAGLHWDYRPFVPGGNSGVNDGITFDIAAQI
                     GALIKTGGSGSPLSGLRVRHVIESGFSQDGGFTFTQADVFHAIERMPGGGPIYDGY"
     gene            complement(1254..2375)
                     /locus_tag="VF516_13045"
     CDS             complement(1254..2375)
                     /locus_tag="VF516_13045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108651.1"
                     /translation="MTARRLISVLTVALSAGAGVSASVDAQPATQPAAKKPARPTKGK
                     AGKKTPLAPAKNSGSATGSGAGSSTGSADAAGAGAGSAVQMAEDPPPSDMTGTAENPD
                     APHSMMDSDSPPAVAVVAAPVRPAGYPIEEALRPITLPQNLSEVSLLPHAQVSPYAAT
                     TALRVRYGVTPKVQIGLTYLIGAIFDDPTTVEPKQGFHSGKAVGLDATVMLQDWIGIQ
                     VGVPLYISPLAVSLALGVPMKFTFGDKFAIGGLDDFLNIKLDRFAPTFYQEVQNATAA
                     AGTMTNTTQSAGELRISLFGVYQYQPNFALIGRAGIQMEDFATGKTNGCAGVCLTTFI
                     SAGLRYTPRKYLDLGLAIGFDDLAHGGSFAPAGYLAFRI"
     gene            2485..3294
                     /locus_tag="VF516_13050"
     CDS             2485..3294
                     /locus_tag="VF516_13050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108652.1"
                     /translation="MALGALQTDDSPTGASIGRATSEQGPDRRDLIAGSTNQRQRRLA
                     LESARSGRHSRRSRQAVREVAAHPEVPSAFVAGPPLTPARAEDRALFKGRGDVIKLIE
                     HDLAPDRRGVLLVVGQRRMGKTSLCNYLPTYLGTGTVVVVSNFQSLSGDAHRETPHRR
                     VLADIAAHAPGTPAPPESDRWGDGLRWLEELDRACTDRRLLAVIDEIERVEDGIRDGW
                     CSSDFLDFLRAAGDALPPHSFSPAHRLSSAPPRFSLDRSAGERHQPHDLVS"
     gene            3502..3729
                     /locus_tag="VF516_13055"
     CDS             3502..3729
                     /locus_tag="VF516_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108653.1"
                     /translation="MVGDVHLFDEIWRSRTDEERAVLQRLARSDEPGELDPAAIPLVR
                     EGYLARMGDKAAFVVPLFREWIGYSSCELTF"
BASE COUNT          590 a         1379 c         1413 g          645 t
ORIGIN      
        1 gtagccgtcg tagatcgggc cgccgccggg catgcgctcg atcgcgtgga acacgtcggc
       61 ctgggtgaac gtgaagccgc cgtcctgcga gaacccgctc tcgatcacgt ggcggacccg
      121 caggccgctc agcgggctgc cgctgcctcc ggtcttgatc agcgcgccga tctgcgcggc
      181 gatgtcgaac gtgatgccgt cgttgacgcc gctgttgccg ccgggcacga acggccggta
      241 gtcccagtgc aggcccgcgt agcgcgccgg gttccactgc ttgagcgcgt tgatgatcac
      301 cgacttcgac gaccagccga tgaagatgtc gccgttggcg gtgaacagct cgcgcgaacg
      361 atcccacacc gcggcgatgt cgaacccgcc gctcgggttg agcgcctcga tcaccacggt
      421 gccgctgaag tcgtggtcgc gcttcgggcg cttgacgatc atccgcgtcg tgtatgggat
      481 gctggtgcag cccgaggtcg ccgcggccgg gcacggcgac accacgcgga tccccgtcgg
      541 cgtgaactcg tagaggttgc cgacgccgga cacgaagaac tcctcgatga cgtagccgtc
      601 gggcaccggc ggctggacat cgaagccttc gatcgcgaac ccgaagttgt cgggtgtcga
      661 cgggatcggg ccggtgacgg tcggcagcgc gacggcgaac ggctggacgt cgtcggccgc
      721 ggcgatcgac gccggcgcga tcaccgcgag cgccgcggcc agcgtggccg cgcggcgccg
      781 tcgtggcagg cgaggggatg gctgcacagg actgcgttgc atgggactcc tcctccggat
      841 cgggttggca gtcggcgcgg cgaggcacct gcgggggggc gcctggcaac ctcgccggcc
      901 gaccggccgg tcgaacccgg cgagcgattg atgcgccgga ttggatcgcg atcgcaagca
      961 gagatttgca gcgcggctcc gtgatgcggc gcggcgcgcg tgcgcgcgca ccagcgcgct
     1021 gcgtcagccc cgttcgccgt cgcgctgctc cgtaagcgcg ctagcggatc gcggtcgctc
     1081 tgcagtcgct ctcacgtcgt gcggtggagc gcgttgcgtt ctgtgcgtct cacgggaacc
     1141 aaggagttcc cgttgcgggg cccatcggct cgtcctgcgt ggcgagatcc gccggcgacg
     1201 ccatccatcc aggaaccagg ccgtaccccg aggtcgggcg cgaagagctt gcgtcagatg
     1261 cggaacgcga ggtagccggc gggcgcgaac gagccgccgt gcgcgaggtc gtcgaagccg
     1321 atcgccaggc cgaggtcgag gtacttgcgc ggcgtgtagc ggagcccggc gctgatgaac
     1381 gtggtgaggc acacgccggc gcagccgttg gtcttgccgg tcgcgaagtc ctccatctgg
     1441 atgcccgccc ggccgatcag cgcgaagttc ggctggtact ggtacacgcc gaacagcgag
     1501 atccggagct cgccggcgga ctgggtcgtg ttggtcatcg tgccggcggc ggcggtcgcg
     1561 ttctgcacct cctggtagaa cgtcggcgcg aagcggtcga gcttgatgtt caggaagtcg
     1621 tcgagcccgc cgatcgcgaa cttgtcgccg aacgtgaact tcatcgggac gccgagcgcg
     1681 agcgacaccg cgagcgggct gatgtacagc ggcacgccga cctggatccc gatccagtcc
     1741 tgcagcatca cggtggcgtc gagcccgacc gccttgccgg agtggaaccc ctgcttgggc
     1801 tcgacggtcg tcggatcgtc gaagatcgcg ccgatcaggt aggtcaggcc gatctggacc
     1861 ttgggcgtga cgccgtagcg cacgcgcagc gcggtggtcg ccgcgtacgg cgagacctgc
     1921 gcgtggggca agagcgagac ctcggacagg ttctgcggca gcgtgatcgg ccgcagcgcc
     1981 tcctcgatcg gatacccggc ggggcggacc ggcgccgcca ccaccgcgac cgccggcggg
     2041 ctgtcgctgt ccatcatcga gtgcggcgcg tcggggttct ccgcggtccc ggtcatgtcc
     2101 gacgggggcg ggtcctcggc catctgcacc gccgagcccg cgccggcgcc cgccgcgtcg
     2161 gccgagcccg tgctggagcc tgcgccggag ccggtggccg agccgctgtt cttggcgggc
     2221 gcgagcggcg tcttcttgcc ggccttgccc ttggtgggcc tggcgggctt cttggccgcg
     2281 ggctgggtcg cgggctgggc atcgacgctg gccgagaccc ccgcgccagc gctcagggcc
     2341 acggtcaaca cggagatcag gcggcgggcc gtcatgcgtg caacattacc tcaaccaggg
     2401 ggtgtcccga ctgtagcgcc gagcgagacc tcccaggcgt gcgccccgat tcgcctcgaa
     2461 ccgtctgggg agatcgaaaa cctgttggcc ctcggcgccc tgcagacgga tgactcccct
     2521 acgggtgcat cgatcggtcg tgctacctcg gagcaaggcc cggatcgccg cgatctcatc
     2581 gccggtagca ccaaccagcg ccagcgccgc ctggcactcg agagcgcgcg cagcggccgt
     2641 cattcgcgaa gaagccgaca agctgtccgc gaagtcgcgg cgcaccccga ggtcccgtcc
     2701 gccttcgtcg ccgggccgcc gctcaccccc gcgcgggccg aggaccgcgc cctcttcaag
     2761 ggacgcggcg acgtcatcaa gctcatcgag cacgatctcg ctcccgatcg ccgcggcgtg
     2821 ctgctggtcg tcgggcagcg caggatgggc aagacgtcgc tgtgcaacta cctgccgacc
     2881 tatctcggta ccgggaccgt cgtcgtggtg tccaacttcc agtcgctgag cggcgacgcg
     2941 caccgcgaga ccccgcaccg gcgcgtcctc gctgacatcg cggcacacgc gcctggcacg
     3001 ccggcgccgc ccgaatcgga tcgctggggc gacggcctgc gctggctcga ggagctcgat
     3061 cgggcctgca ccgatcggag gctgctggcc gtcatcgacg agatcgagcg ggtcgaggat
     3121 gggatccggg atggctggtg ctccagcgac ttcctcgact tcctgcgcgc cgccggcgac
     3181 gccctcccac cacattcgtt ttctcctgct caccgcctat cttctgcacc gcctcggttc
     3241 tcactggacc gatcggctgg tgagcgccac cagccgcacg atctcgtatc ttgacgagga
     3301 cgacgcccgg gagctcctcg ctcaccccat ccccgagttc cccgagatct accccgaggg
     3361 cggtatcgac ctcatctgcc gcagaccggc cggcatccct acctgctgca gaaggccggc
     3421 gatgacctct gccgcctgct gaacagccgc ggaggtctcc gcaccgccac ccgcggggag
     3481 ctcaccgagg tgttcgatgg catggtcggc gacgtccacc tgttcgacga gatctggcgc
     3541 tcgcgcaccg atgaggagcg cgccgtgctc cagcgactcg ctcggtccga cgagccgggc
     3601 gagctcgatc ccgccgcgat cccgctcgtg cgcgaaggct atctcgcacg catgggtgac
     3661 aaggcggcct tcgtcgtccc gctgttccga gaatggatcg gctactcgag ctgcgagcta
     3721 acattttgat atctttggct tctgcttcgt gactcaaacc tgatggttac gtagggttac
     3781 ggctcgctac gtcaacgacg gggccatgtg attctcgatg cttgcgcaga gccgacggag
     3841 gggtggtctt ggtgcgcgga acgaagagct cgagttcagg tccggtgcag cacgaacctc
     3901 gggccacggt atggtggtgg gttcaccatg tcactcgaac aacttcgacc aacgacgaaa
     3961 ccgcgcctga tagatcttgt cgcgcaagcg gggatcgacg tcacaccttg ggcgtcgacg
     4021 gcggcgg
//
LOCUS       DASVOR010000660         4437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_414896,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000660 DASVOR010000000
VERSION     DASVOR010000660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414896"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2298)
                     /locus_tag="VF516_13060"
     CDS             complement(124..2298)
                     /locus_tag="VF516_13060"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8108654.1"
                     /translation="MRKLLLITVVACGGGTQSAHVPVDPPAHPIAEAPAKAPEVSMPA
                     QHSHPWPTTRVATITDTLHGQKITDPYRWLEDETSSEVQAWMTAQDGYARGVLGGSPH
                     RDDWVARLKQVFYFDAITAPVHRGGRYFYSRKHADKEKTVVYWKQGEAGAEQVLFDPN
                     QWSTDGSKGLGVTSASWDGRYVAYGVKEHNSDEAVLHVHDVAAGKDLADTLEGAKYAN
                     PSWTPDNHGFYYTWVPPVGGAVTVADRPGFAEIRYHKLGTPVASDPVVHAATGNPGTF
                     IHGEVSRDGHWLIVVIQHGWNSTDVYVKDTRKHGAGWTALVEGVDAIFDLTAWRDQFY
                     VMTNHGAPKNRLYKVDPAHLEQAAWKEIVPESDATLEHASVVGEHLVLTYLRHAASEI
                     EIHDLAGKLVHKVDLPPLGSAAGIVGNPDEDTGYIAYTSFTEPQVIYKTSIRTGKTAE
                     WARIKLPVDTAQLTAEQVFYPSKDGTKISMFILSKKGTARDGTHPTILTGYGGFNVSM
                     TPAFASSRAVWLERGGVIAIPNLRGGGEYGEDWHRAGMMLVKQNVFDDFIAAAEFLVR
                     EHYTTPAQLAAKGRSNGGLLMGAVVNQRPDLFAAANAGVGVLDMLRYHKFGIAYAWAG
                     DYGTSDTAEGFKTLYAYSPVHNVRAGQRYPAVLVTTADHDDRVHPLHSFKYTAALQAA
                     QAGDKPILIRVETRAGHGPGTALSKQIEENADVLSFLERYTR"
     gene            complement(2343..2795)
                     /locus_tag="VF516_13065"
     CDS             complement(2343..2795)
                     /locus_tag="VF516_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108655.1"
                     /translation="MALALTSRRSTLAAVASALAAMALAAAVAGRSCRVRQPGPEAAV
                     RDLLQAAKTGDRDAVFELLSSATQDRLAAQARHATDLVGAAQRYTARDLISIGSSDGV
                     SDPADVTVVDEQADRAAVEVVSPAGRSRIDLVKQGGRWRIDLPQYAAP"
     gene            complement(2780..3793)
                     /gene="trpS"
                     /locus_tag="VF516_13070"
     CDS             complement(2780..3793)
                     /gene="trpS"
                     /locus_tag="VF516_13070"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEX8108656.1"
                     /translation="MKTVFSGIQPTGELHLGNYLGAVRNWVALQDEYRCIFCVVDYHA
                     LTQDYDPKEMAKRVTEMVIDLIALGIDPERSLLFLQSMVPEHTELAWVFTNVTGFGDL
                     ERMTQFKDKAENQPDNVNVGLFQYPVLQAADILLYKAERVPVGQDQVQHIELARRIAR
                     SFNHRWGKVFPECEPLLTQLPKLLGLDGKKKMSKSLGNHIPLALFGGAAADPARAAAG
                     VKKLLGRAVTDEKRVSRDDPGNPDDCNIATIHRAMSSAEDLAWMHLGCTTAAIGCVDC
                     KARLADTMTAQFAGYAARRAELIAAPQRVQDILEQGAAKARAIAQRTMGEVHRKLGLW
                     RSR"
     gene            3920..>4437
                     /locus_tag="VF516_13075"
     CDS             3920..>4437
                     /locus_tag="VF516_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108657.1"
                     /translation="MGSLALILGVTACVVHDTPPVNPGYGYYGPQSYGPPPGGYGPGY
                     GSEDAAGNGAVGEPTPYSVDSMPPEPLYEQMTPSPGESFVWIDGYWHWNGYEWVWVNG
                     RWEHDQAGYVYVEPNYDYVGDQFIYTPGYWAPPTRIPRGWHVRDHRNGRPAIVSPPGV
                     GGGAGPPVLGTRP"
BASE COUNT          609 a         1523 c         1578 g          727 t
ORIGIN      
        1 ccgcaagttc gagcgccgct acggccacgt gatggcgcac gcgcgctcgc tgccgcgcaa
       61 gggcgatccg cgacggctgg cggcgatgga agcgctgtgg caagaggcca agcgccggga
      121 gcgctagcgc gtgtagcgct ccaggaacga cagcacgtcc gcgttctcct cgatctgctt
      181 cgacagtgcc gtgccggggc cgtgaccggc gcgggtctcg acccggatca ggatcggctt
      241 gtcgccggcc tgcgccgcct gcagcgcggc cgtgtacttg aacgagtgca gcgggtgcac
      301 ccggtcgtcg tggtccgcag tggtcaccag caccgcggga tagcgctggc cggcgcgaac
      361 gttgtgcacg ggtgagtagg cgtagagcgt cttgaagccc tcggcggtgt cggaggtgcc
      421 gtagtcgccg gcccaggcgt aggcgatgcc gaacttgtgg tagcgcagca tgtcgagcac
      481 gccgacgccc gcgttggcgg cggcgaacag gtccggccgc tggttgacca cggcgcccat
      541 cagcagcccg ccgttcgagc ggcccttcgc ggccagctgg gccggcgtcg tgtagtgctc
      601 gcggaccagg aactcggccg cggcgatgaa gtcgtcgaac acgttctgct tgaccagcat
      661 catgccggcg cggtgccagt cttccccgta ctcgccgccg ccgcgcaggt tggggatcgc
      721 gatcaccccg ccgcgctcga gccacaccgc gcgcgagctc gcgaacgccg gcgtcatgct
      781 gacgttgaac ccgccgtagc cggtgaggat cgtcgggtgg gtcccgtcgc gcgcggtgcc
      841 cttcttcgac aggatgaaca tcgagatctt cgtgccgtcc ttcgacggat agaacacctg
      901 ctcggcggtg agctgcgcgg tgtcgaccgg cagcttgatc cgcgcccact cggccgtctt
      961 gccggtgcgg atcgacgtct tgtagatcac ctgcggctcg gtgaacgacg tgtaggcgat
     1021 gtagccggtg tcctcgtcgg ggttgccgac gatgccggcc gcgctgccga gcggcggcag
     1081 gtcgaccttg tggaccagct tgccggcgag gtcgtggatc tcgatctcgc tcgcggcgtg
     1141 gcgcaggtag gtcagcacca ggtgctcgcc gacgacgctg gcgtgctcca gcgtcgcgtc
     1201 gctctcgggg acgatctcct tccacgccgc ctgctcgagg tgcgcggggt cgaccttgta
     1261 caggcggttc ttcggcgcgc cgtggttggt catcacgtag aactggtcgc gccacgcggt
     1321 caggtcgaag atcgcgtcga cgccctcgac cagcgccgtc cagcccgccc cgtgcttgcg
     1381 ggtgtccttg acgtagacgt cggtcgagtt ccagccgtgc tggatcacga cgatcagcca
     1441 gtggccgtcg cgcgacacct cgccatggat gaacgtgccg gggttgccgg tcgcggcatg
     1501 gacgaccgga tcgctggcca ccggcgtccc gagcttgtgg tagcggatct cggcgaaccc
     1561 cgggcggtcc gcgaccgtca ccgcgccgcc gaccggcggg acccacgtgt agtagaagcc
     1621 gtggttgtcg ggtgtccacg acgggttcgc gtacttggcg ccctcgagcg tgtccgccag
     1681 gtccttgccc gcggcgacgt cgtggacgtg gagcacggcc tcgtcggagt tgtgctcctt
     1741 cacgccgtac gcgacgtagc ggccatccca gctcgccgag gtcacgccga ggcccttgct
     1801 gccgtcggtg ctccactggt tgggatcgaa cagcacctgc tcggcgccgg cctcgccctg
     1861 cttccagtag acgacggtct tctccttgtc ggcgtgcttg cgcgagtaga agtaccggcc
     1921 gccgcggtgg accggcgcgg tgatcgcgtc gaagtagaac acctgcttga gccgcgcgac
     1981 ccagtcatcg cggtgcggcg agccgccgag cacgccgcgc gcgtagccgt cctgcgcggt
     2041 catccaggcc tggacctccg acgaggtctc gtcctcgagc cagcgatacg gatccgtgat
     2101 cttctggccg tgcagggtgt cggtgatggt ggcgacccgc gtggtcggcc acggatggga
     2161 gtgctgggct ggcatcgaga cctcgggagc cttcgcggga gcttccgcga tggggtgcgc
     2221 cggcgggtcg accggcacgt gggcgctctg cgttcctccc ccacatgcga cgaccgtgat
     2281 cagaaggagc ttgcgcatgc tgcgtgatac accgaagccc ctggcgaggt cgagcgtcgc
     2341 cgctacgggg cggcatattg tggaagatcg atccgccagc ggccaccctg cttgaccagg
     2401 tcgatccggg accggccggc cggcgacacg acctcgaccg ccgcgcgatc ggcctgctcg
     2461 tcgaccacgg tgacgtcggc cggatccgac acgccgtcgg agctgccgat cgagatcagg
     2521 tcccgggcgg tgtagcgctg cgctgcgccg accaggtcgg tcgcatggcg ggcctgggcc
     2581 gcgagccggt cctgggtcgc cgacgacagc agctcgaaca ccgcgtcgcg atcgccggtc
     2641 ttggcggcct gcagcagatc gcgcaccgcg gcctcggggc cgggctgcct cacccggcag
     2701 ctgcggccgg ccaccgcggc ggcgagcgcc atcgcggcca gcgcgctggc gaccgcggcc
     2761 aacgtcgagc gccgtgaggt cagcgcgagc gccatagccc gagcttcctg tggacctcgc
     2821 ccatcgtgcg ctgcgcgatc gcgcgcgcct tcgccgcccc ctgctccagg atgtcctgca
     2881 cgcgctgcgg cgccgcgatg agctccgcgc ggcgcgcggc atagccggcg aactgcgcgg
     2941 tcatcgtgtc ggcgagccgg gccttgcagt cgacgcagcc gatcgcggcg gtggtgcagc
     3001 cgaggtgcat ccacgccagg tcctcggccg acgacatcgc gcggtggatc gtcgcgatgt
     3061 tgcagtcgtc ggggttgccg ggatcgtcgc gcgacacccg cttctcgtcg gtgacggccc
     3121 ggccgagcag cttcttgacc ccggcggccg cgcgcgccgg atcggcggcg gcaccgccga
     3181 acagcgcgag cgggatgtgg ttgccgagcg acttgctcat cttcttcttg ccgtcgaggc
     3241 cgagcagctt gggcagctgg gtgagcagcg gctcgcactc ggggaacacc ttgccccagc
     3301 ggtggttgaa gctgcgcgcg atccggcgcg ccagctcgat gtgctgcacc tggtcctgac
     3361 cgaccggcac gcgctcggcc ttgtacagca ggatgtcggc ggcctgcagc accgggtact
     3421 ggaacagccc gacgttgacg ttgtcgggct ggttctccgc cttgtccttg aactgcgtca
     3481 tccgctccag gtcgccgaac ccggtgacgt tggtgaacac ccaggcgagc tcggtgtgct
     3541 cggggaccat cgactgcagg aacagcagcg accgctccgg gtcgatgccc agcgcgatga
     3601 gatcgatcac catctcggtg acgcgcttgg ccatctcctt ggggtcgtag tcctgggtca
     3661 acgcgtgata atcgacgacg cagaagatgc agcggtactc gtcttgcagc gccacccagt
     3721 tgcgaaccgc gccgagatag tttcccagat gcagctctcc cgttggctgg atacccgaga
     3781 acaccgtttt catcggcgtt ctgtgtactt gtggcgcgat ctcccgtcaa ctagactcgc
     3841 cgggcccggt ctcggcgcgc tggggaaacc tcgcagcccg gtggaggaag ccatgcagat
     3901 ccgacgcttt gctcggtggg tggggagctt ggcgctgatc ctgggggtga cggcctgcgt
     3961 cgttcacgac acgccgcctg tgaatcctgg ttacgggtac tacggcccgc agagctacgg
     4021 cccgcccccc ggaggctacg gaccaggcta cggctccgag gacgccgccg gcaacggcgc
     4081 ggtcggcgag ccgaccccgt acagcgtcga cagcatgcca cccgagccgc tctacgagca
     4141 gatgacgccg tcgcccggcg aaagcttcgt ctggatcgac ggctactggc actggaacgg
     4201 ctacgagtgg gtctgggtca acggccgctg ggagcacgat caggccggct acgtctacgt
     4261 cgagccgaac tacgactacg tcggcgatca gttcatctac acgccgggct actgggcgcc
     4321 gccgacgcgg atcccgcgcg gctggcacgt ccgcgaccac cgcaacggcc ggccggcgat
     4381 cgtctccccg ccgggggtcg gcggtggcgc ggggccgccc gtgctcggca cccggcc
//
LOCUS       DASVOR010000661         4104 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_123129,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000661 DASVOR010000000
VERSION     DASVOR010000661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4104
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123129"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VF516_13080"
     CDS             complement(<1..676)
                     /locus_tag="VF516_13080"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX8108658.1"
                     /translation="MACTDANTLGAWLAGALPEAERTAIEDHAAVCDRCHALVQGFAA
                     TRPSSHAARGALTSLTSQVTGTLEPAPEPSEGTPTLPARPGAGTSEFTGTDRFEVVRR
                     IGAGAMGVVYEVWDRERRARFALKTLPRLSWDRLSLFKNEFRVLRGLSHRNLVTLGEL
                     VKDREDWFFTMELVDGAPFLAWACPSGRAPTHPDAPGSGLPAAASGCSAFAFDEARLR
                     DGLRQLA"
     gene            complement(1075..1467)
                     /locus_tag="VF516_13085"
     CDS             complement(1075..1467)
                     /locus_tag="VF516_13085"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8108659.1"
                     /translation="MTAVTPGDPTVWRRHPHAPAPGTELGPAGAIPDGQGREFAFGPE
                     PDAFRMFVVRRGSEVFGYVNVCPHFSLRLNYRPDQFTTRDGEILCSMHFALFRMEDGV
                     CIDGACPGRALDPVPVEVTPGGTLRIAG"
     gene            complement(1483..2946)
                     /locus_tag="VF516_13090"
     CDS             complement(1483..2946)
                     /locus_tag="VF516_13090"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8108660.1"
                     /translation="MQTKLFIGGEFVDGLAGGTIDVMNPFDGEKLADVAEARAEDVDR
                     AVDAAARAFPAWSRTAPSERGRLLFALASAVEANAARLAELESRDTGHPIRDSANLDV
                     PRTVLGFRYFAGMADKIEGSVIPVDPGFLNYVEREPLGVVGQIVPWNFPLMFTSWKLG
                     PALAAGNTVVLKPAELTPLSSLAIAELVREVGFPPGVVNIIPGYGATAGQRLAEHDRV
                     AKLSFTGSTATGRKLVAASAGNLKKLQLELGGKGPNIVFDDAELDAAVAGSAFALFHN
                     QGQACIAGTRILLHAAIADRFLDAFLALARSIRLGDPLDKRTEMGPLTSRLHQERVLG
                     YCEIARREGGEFLIGGKAPSDPALARGCFVEPTLVRAAPHHTVCRDEVFGPFATVTTF
                     SDEDEAIRIANDTRYGLGAGLWTRDVKRAHRVAKAIRSGMVWVNCYKRVHPGSPFGGV
                     GESGYGREMGFEVMREYTQPKSVWINIDAKIPPFYPR"
     gene            complement(2948..>4104)
                     /locus_tag="VF516_13095"
     CDS             complement(2948..>4104)
                     /locus_tag="VF516_13095"
                     /inference="COORDINATES: protein motif:HMM:NF036733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108661.1"
                     /translation="AAYRLRAAAPAAVVFQTIEGPETICRWAEICQTDGGRAQAAASA
                     PAAAKPAAAARPALALQTRFETGAPDPDTGYRSFRAGGYAFHRDRYFAYISHGDVHHQ
                     MPADAFLRALMRDVAWGFFYGTVNFDDVLGTTNFYGEVEMFLGAKNEAFQRAGRDVTE
                     RFASADLMKTFKAILHDWTNEGFDPFAAPLETAAAFGAKHGSNNAAIERQRVSARRMV
                     GLPGDTGLRTDTAGYPVNRMFADVPQDQPRVEVEPGCEHDVAAFNLFGYLSRSDVTWN
                     PSVCSVLRDSLFCPTSEEYILPIDHGNDRVEWFIQLSDEIVWDVKDGATGRPRARVTM
                     RAGDVAAMPADIRHRGFSTKRSMLLVWENGSPAIPELIRTGKAPVVPVEF"
BASE COUNT          569 a         1426 c         1523 g          586 t
ORIGIN      
        1 ccgcgagctg gcgcaggccg tcgcgcagcc gcgcctcgtc gaacgcgaac gcgctgcacc
       61 ccgacgcggc ggcgggcagc ccggagcccg gcgcgtcggg gtgggtcggc gcgcggccgc
      121 tcgggcacgc ccacgccagg aacggcgcgc cgtcgaccag ctccatggtg aagaaccagt
      181 cctcgcggtc cttgaccagc tcgccgagcg tgacgaggtt ccggtgcgac agcccgcgca
      241 gcacgcggaa ctcgttcttg aacagcgaca gccggtccca cgacagccgg ggcagcgtct
      301 tgagcgcgaa ccgcgcccgg cgctcgcgat cccacacttc atagaccacg cccatcgcgc
      361 ccgcgccgat ccgccggacg acctcgaagc ggtcggtgcc ggtgaactcc gacgtcccgg
      421 cgccgggtcg cgcgggcagg gtcggggtgc cctcggacgg ttcgggggcc ggctccagcg
      481 tgccggtcac ctggctggtg aggctcgtca gcgcgccgcg cgccgcgtgg ctcgacgggc
      541 gggtcgccgc gaagccctgg accagcgcgt ggcagcggtc gcacacggcc gcgtggtcct
      601 cgatcgccgt gcgctcggcc tccggcagcg cgccggccag ccacgccccc agggtgttcg
      661 cgtcggtgca tgccacgacg gttccccagt gtagcgcctc cgcgcccggt gcgatgtgag
      721 gaatctgtga caccgggagc gaaccacctc acgctgccgc gggcagcggg caatcaagcg
      781 ctgtcgatca agggttgggg ccttgcctcg ggtgcgccag ccgtccggtg gacgtcgacc
      841 tcggccttga cgttgacggc cgtcgttgag cttgacgtgg ccgtggacgt tgagcccatc
      901 gtcgaccttc acgttgacca tcggtcgcga ttcttcgacg aggatcggag accaccagaa
      961 aaggtcaagg tcgaggatgg agtcgacgtc gccgttcagg tcaatgcctt gcgacaacgt
     1021 caaggtcgac ctcaacgtct tcgcaaggac gttccggcct tcatcgacac ctcttcaacc
     1081 cgcgatccgc agcgtgccgc cgggcgtgac ctcgacgggc accggatcga gcgcgcggcc
     1141 cgggcacgcg ccgtcgatgc acacgccgtc ctccatgcgg aacagcgcga agtgcatgct
     1201 gcacaggatc tcgccgtcgc gcgtggtgaa ctgatccggc cggtagttga gccgcaggct
     1261 gaagtgcggg cagacgttga cgtagccgaa cacctcgctg ccgcgccgca cgacgaacat
     1321 ccggaacgca tcgggctcgg ggccgaacgc gaactcgcgg ccctgcccgt cggggatcgc
     1381 gccggccggg ccgagctcgg tgccgggtgc cggcgcgtgc gggtggcggc gccacaccgt
     1441 cggatcgccg ggcgtcaccg ccgtcaccgc atcaccgggc cgtcaccggg gatagaacgg
     1501 ggggatcttg gcgtcgatgt tgatccacac cgacttgggc tgggtgtact cgcgcatgac
     1561 ctcgaagccc atctcgcggc cgtagccgga ctcgccgacg ccgccgaacg gcgagccggg
     1621 gtggacgcgc ttgtagcagt tgacccagac catgccgctg cggatcgcct tggcgacccg
     1681 gtgggcgcgc ttgacgtcgc gggtccacag cccggcgccc aggccgtagc gggtgtcgtt
     1741 ggcgatccgg atcgcctcgt cctcgtcgct gaacgtcgtg acggtggcga acgggccgaa
     1801 cacctcgtcg cggcacaccg tgtggtgcgg cgcggcgcgc acgagggtcg gctcgacgaa
     1861 gcagccgcgg gcgagcgccg gatcgctcgg cgccttgccg ccgatcagga actcgccgcc
     1921 ctcgcggcgc gcgatctcgc agtagccgag cacgcgctcc tggtgcaggc ggctggtcag
     1981 cgggcccatc tcggtccgct tgtcgagcgg gtcgccgagc cggatgctcc gggcgagcgc
     2041 gaggaacgcg tcgaggaagc ggtcggcgat cgcggcgtgg agcaggatcc gcgtgccggc
     2101 gatgcaggcc tggccctggt tgtggaacag cgcgaacgcg ctgccggcga ccgcggcgtc
     2161 gagctcggcg tcgtcgaaca cgatgttggg gcccttgccg ccgagctcga gctggagctt
     2221 cttgaggttg ccggccgagg cggcgaccag cttgcggccg gtcgcggtcg acccggtgaa
     2281 cgacagcttg gcgacccggt cgtgctcggc gagccgctgg ccggcggtgg cgccgtagcc
     2341 cgggatgatg ttgaccacgc cgggcgggaa cccgacctcg cggaccagct cggcgatcgc
     2401 gagcgagctg agcggggtga gctcggccgg cttgagcacg acggtgttgc cggcggcgag
     2461 cgcgggcccg agcttccagc tggtgaacat cagcgggaag ttccatggca cgatctggcc
     2521 gaccacgccg agcggctcgc gctcgacgta gttgaggaac ccggggtcga ccgggatcac
     2581 cgagccctcg atcttgtcgg ccatgccggc gaagtagcgg aacccgagga ccgtgcgcgg
     2641 cacgtcgagg ttggccgagt cgcggatcgg gtggccggtg tcgcgcgact cgagctcggc
     2701 gagccgcgcc gcattggcct cgaccgcgct ggccagcgcg aacaagaggc gcccgcgctc
     2761 gctcggcgcg gtccggctcc acgccgggaa cgcgcgcgcg gccgcgtcga ccgcgcgatc
     2821 gacgtcctcg gcgcgggcct cggcgacgtc ggcgagcttt tcgccgtcga acgggttcat
     2881 cacatcgatc gtgccgccgg cgaggccgtc gacgaactcg ccgccgatga acagcttggt
     2941 ctgcatgtca gaactcgacc gggaccaccg gggccttgcc ggtgcggatc agctcgggga
     3001 tcgcgggcga gccgttctcc cagaccagca gcatcgagcg cttggtcgag aacccgcggt
     3061 ggcggatgtc ggcgggcatc gcggcgacgt cgcccgcccg catggtgacc cgggcgcgcg
     3121 gccggccggt ggcgccgtcc ttgacgtccc acacgatctc gtcggacagc tggatgaacc
     3181 actcgacgcg gtcgttgccg tggtcgatcg gcaggatgta ctcctcggag gtcgggcaga
     3241 acaggctgtc gcgcagcacc gagcacacgc tcgggttcca cgtcacgtcg gagcgcgaca
     3301 ggtagccgaa caggttgaac gccgcgacgt cgtgctcgca cccgggctcg acctcgaccc
     3361 gtggctggtc ctgcgggacg tcggcgaaca tccggttgac cgggtaaccg gcggtgtcgg
     3421 tgcgcagccc ggtgtcgccg ggcaggccga ccatgcggcg cgcgctgacc cgctggcgct
     3481 cgatcgcggc gttgttgctg ccgtgcttgg cgccgaacgc cgcggcggtc tcgagcggcg
     3541 ccgcgaacgg atcgaagccc tcgttggtcc agtcgtgcag gatcgccttg aacgtcttca
     3601 tcaggtcggc ggacgcgaac cgctcggtga cgtcgcgccc ggcgcgctgg aacgcctcgt
     3661 tcttggcgcc gaggaacatc tcgacctcgc cgtagaagtt ggtcgtcccg agcacgtcgt
     3721 cgaagttgac cgttccgtaa aagaagcccc acgcgacgtc gcgcatcagc gcgcggagga
     3781 acgcgtcggc cggcatctgg tggtggacgt cgccgtggct gatgtacgcg aagtagcggt
     3841 cgcggtggaa cgcgtagcca ccggcgcgga agctccggta gcccgtgtcg gggtcgggcg
     3901 cgccggtctc gaaccgggtc tgcagggcga gcgccggcct tgcggcggca gccggtttcg
     3961 cggccgccgg agcgctcgcc gcggcctggg cgcggccgcc gtcggtctgg cagatctcgg
     4021 cccaccggca gatcgtctcg gggccctcga tggtctggaa caccacggcg gccggcgccg
     4081 cggcgcgcag ccggtacgcg gcct
//
LOCUS       DASVOR010000662         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_444503,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000662 DASVOR010000000
VERSION     DASVOR010000662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444503"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VF516_13100"
     CDS             <1..1213
                     /locus_tag="VF516_13100"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8108662.1"
                     /translation="AMRAELPAVRRIVVLADDHAASLARWLTGHGDADPGVAGSPDDV
                     VFQLYTSGTTGSPKGAQLTHRSFFDLWNGMARQGDPWLGLGPRDVLLIPLPSFHIAGV
                     WWMVRGLAAGSACIVMEAFVGWQVLELIARHRVTRLFAVPAMLQVMLAEPSCRTADLS
                     SLTHIVYGASPAAPALLQQAMATFRCAMVQVYGMTETGNVAVSLRAEDHTLPAGPRMR
                     SAGRALPGVALKIIDAQGRPLGPGQIGEICIHSPANMIGYWNLDAATAETLRDGWIHS
                     GDAGFLDDDGYLFIQDRIKDMIITAGENIYPVEIENALHDHPAVAEAAVIGVPDPRWG
                     EVVKAVVVLRPGAAATAAEILEFLRGRIADFKLPRSVDFAAALPRTPSGKVKKAELRA
                     PYWQGRDRMVN"
     gene            1214..>2226
                     /locus_tag="VF516_13105"
     CDS             1214..>2226
                     /locus_tag="VF516_13105"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEX8108663.1"
                     /translation="MAEAQRTDHAQQLRRALLAIEKAEARADKLEAARREPIAVVGLG
                     CRFPRATTPSEFWDNLVGGVDCIREVPADRWDVDALFDPDPDAPGKMITRWGGFIDGP
                     DQFDPAFFGIAPREAEAIDPQQRIALEVAWEALEDAGLVPAELAGTAVGVFMGACLSD
                     YAQHLAADLTAIDAHSATGVALSIIANRISYLLNLKGPSMAVDTACSSSLVAIHLACQ
                     SLRAGECDVALAGGVNMILTPHEMVFFSRARAMARDGRCKAFDARADGFVRGEGGGVV
                     VLKRASDARATGDRVLALVRGSAVNQDGASNGMTAPNGPSQQRVIRQALASAGLRPSD
                     VDAV"
BASE COUNT          302 a          779 c          831 g          314 t
ORIGIN      
        1 ggcgatgcgc gcggagctcc cggccgtgcg acgcatcgtg gtcctggcgg acgaccacgc
       61 cgcgtcgctg gcgcgctggc tcaccggtca cggcgacgcc gatccgggcg tggccggctc
      121 gcccgacgac gtcgtgttcc agctctacac cagtggcacc accggcagcc ccaagggcgc
      181 gcagctcacc catcgcagct tcttcgatct gtggaacggc atggcgcggc agggcgatcc
      241 atggctcggg ctcgggccgc gcgacgtgct gttgatcccg ctgcccagct tccacatcgc
      301 cggcgtgtgg tggatggtcc gcggcctggc cgccgggtcc gcgtgcatcg tcatggaagc
      361 gttcgtcggc tggcaggtgc tggagctgat cgcgcgccat cgggtcaccc ggctgttcgc
      421 ggtgccggcg atgctccagg tgatgctcgc cgagccgagc tgccggaccg ctgacctgtc
      481 gtcgctgacc cacatcgtct atggcgcgtc gccggccgcc ccggcgctgc tccagcaggc
      541 gatggcgacg ttccgctgcg cgatggtgca ggtctacggc atgaccgaga ccggcaacgt
      601 cgcggtgtcg ctgcgcgccg aggaccacac actgccggcc ggcccgcgga tgcgctcggc
      661 gggccgcgcg ctgcccggcg tggcgctgaa gatcatcgac gcccagggcc gcccgctcgg
      721 accgggccag atcggcgaga tctgcatcca ctcacccgcg aacatgatcg gctactggaa
      781 cctcgacgcg gcgacggcgg agaccctgcg cgatggctgg atccactccg gcgacgccgg
      841 gtttctcgac gacgacggct acctgttcat ccaggaccgc atcaaggaca tgatcatcac
      901 cgcgggggag aacatctatc cggtcgagat cgagaacgcg ctccacgacc accccgcggt
      961 ggcggaggcg gcggtgatcg gcgtgccgga ccctcgctgg ggcgaggtgg tgaaggcggt
     1021 ggtggtgcta cggccgggcg ccgcggcgac cgcggcggag atcctcgagt tcctgcgcgg
     1081 ccggatcgcc gacttcaagc tgccccggtc ggtcgacttc gcggcggcgc tgccacgcac
     1141 gccgagcggc aaggtcaaga aggccgagct gcgcgctccg tactggcagg gccgcgaccg
     1201 catggtgaac tgaatggccg aggcccagcg gaccgatcat gcccagcagc tccggcgcgc
     1261 cctgctcgcg atcgagaagg ccgaggcgag ggctgacaag ctggaggcgg cgcggcgcga
     1321 gccgatcgcg gtggtcggtc tcggctgccg gttcccgcgg gcgacgacgc cgtcggagtt
     1381 ctgggacaac ctggtcggcg gcgtcgactg catccgagag gtgcccgccg atcgctggga
     1441 cgtcgacgcg ctgttcgatc ccgaccccga tgcgcccggc aagatgatca cgcggtgggg
     1501 cggtttcatc gacgggccgg accagttcga cccggcgttc ttcgggatcg cgccgcgcga
     1561 ggccgaggcg atcgatccgc agcagcggat cgcgctggag gtggcgtggg aggcgctgga
     1621 ggacgccggg ctcgtgccgg cggagctcgc cggcaccgcg gtcggcgtgt tcatgggagc
     1681 gtgcctcagc gactacgcgc agcacctcgc cgccgacctc acggcgatcg acgcccacag
     1741 cgcgaccggg gtggcgctca gcatcatcgc caaccggatc tcgtacctgc tcaacttgaa
     1801 gggcccgagc atggcggtcg acaccgcgtg ctcgtcgtcg ctggtcgcga tccacctggc
     1861 gtgccagagc ctgcgcgccg gcgagtgcga cgtggcgctg gccggcggcg tgaacatgat
     1921 cctcacgccc cacgagatgg tgttcttctc gcgggcgcgc gcgatggcgc gggacgggcg
     1981 ctgcaaggcg ttcgacgcgc gcgccgacgg gttcgtccgc ggcgagggcg gcggcgtggt
     2041 cgtgctcaag cgcgcctcgg acgcccgtgc caccggagac cgcgtgctcg cgctggtgcg
     2101 cggctcggcg gtgaaccagg acggcgcgag caacgggatg acggccccga acggcccctc
     2161 ccagcaacgg gtgatccggc aggcgctggc ctcggccggc ctgcgcccgt ccgacgtgga
     2221 cgcggt
//
LOCUS       DASVOR010000663         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_561933,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000663 DASVOR010000000
VERSION     DASVOR010000663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561933"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            126..1451
                     /locus_tag="VF516_13110"
     CDS             126..1451
                     /locus_tag="VF516_13110"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8108664.1"
                     /translation="MPLARKLGPLDATMIVVSGIIGSGIFTTPYLVAKSVETPFLILA
                     VWVLGGVIALCGAYVFAELGTVVPRAGGQYAFFREAFHPLVGFLHGWSLLWIIQSGAT
                     AFVALQCGEYLVKLARLPSSMITPVAIALLLALVVLHGSGIKPGAIVINVITIGKTLA
                     IAALVLGAFFIAGRSGLSFEPLAPPRLHGPGLLSALFAGLAPAMFTYGGWQNSNFVVE
                     EVRDPERTLPRAILLGVAIVIVVYLSSNVAYVHVLSAPVLAATRTPAADLAGQVLGPR
                     GADAQSVLAIISTFGFLNLALMTAPRVYYAMARDGLFFETVARVSPRSHVPVAAIATQ
                     GVLAALFTLGNAYDWLVGYAVFADWIFFALAGVALMVFRRTRPDAPRPYRAPLYPVLP
                     LVFTLAGVGIVANMFVADTRNALIGSAIITAGVPVYLAWRWWRGRHDAR"
     gene            complement(1455..>1827)
                     /locus_tag="VF516_13115"
     CDS             complement(1455..>1827)
                     /locus_tag="VF516_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108665.1"
                     /translation="AGRRGEAVEYFQLRMVGLPEPMVAQLRHAPFRPQLEAMAHTLVY
                     DATIVGDGRLPVARARRVPCPTLAIAGAAHPFLRETAEALARVVPHSRALILESATHD
                     LVPEVLAPPLLAFFAGDAPAR"
BASE COUNT          218 a          645 c          660 g          304 t
ORIGIN      
        1 cgctcgccca ggcgctggcg cgcaacacag gacggcgcag cccggtgtcc tgaggcgccc
       61 ggcggccgcg cactggggcg ccgccggcct cgtattccgc cgccgggccg gcgtaccttc
      121 ggggcatgcc gctcgcgcgc aagctcggtc cgctcgacgc cacgatgatc gtggtgagcg
      181 gcatcatcgg atccggaatc ttcaccacac cgtacctcgt ggcgaagagc gtcgagacgc
      241 cattcctgat cctcgccgtg tgggtgctcg gcggcgtgat cgcgctgtgc ggcgcgtacg
      301 tgttcgccga gctcgggacc gtcgtgccgc gcgccggtgg gcagtacgcg ttcttccgcg
      361 aggcgttcca tccgctggtc ggcttcctgc acggctggtc actgctatgg atcatccagt
      421 cgggtgcgac cgcgtttgtg gcgctgcaat gcggcgagta tctcgtgaag ctcgcccggc
      481 tgccgtcctc gatgatcacg ccggtcgcga tcgcgctgct gctcgcgctg gtagtgctgc
      541 acggaagtgg catcaagccg ggcgccatcg tgatcaacgt gatcacgatc ggcaagacgc
      601 tggcgatcgc ggcgctggtg ctcggcgcgt tcttcatcgc cggtcgctcg gggctgagct
      661 tcgagccgct ggcgccgccg cggctgcacg gtccgggcct gctctcggcg ctgttcgccg
      721 gcctggcgcc ggcgatgttc acctacggcg gctggcagaa ctccaacttc gtcgtcgaag
      781 aggtgcgcga tcccgagcgc actctgccgc gcgcgatcct gctcggcgtg gcgatcgtga
      841 tcgtcgtgta cctgtcgtcc aacgtggcct acgtgcacgt gctgtccgcg ccggtgctcg
      901 ccgcgacccg gaccccggcg gccgatctcg ctggccaggt gctcggcccg cgtggcgccg
      961 acgcgcagag cgtcctggcg atcatctcga cgttcgggtt tctcaacctc gcgctgatga
     1021 ccgcgccgcg ggtctattac gcgatggcgc gcgacggcct gttcttcgag accgtggcgc
     1081 gcgtctcgcc gcgaagccat gttccggtcg ccgcaatcgc gacccagggc gtgctggccg
     1141 cgctgttcac gctcggcaac gcctacgact ggctggtcgg ctacgcggtg tttgccgact
     1201 ggatcttctt cgcgctcgcc ggcgtcgcgc tcatggtgtt ccggcggacc cggcccgacg
     1261 cgccgcggcc gtatcgcgcg ccgctctacc cggtgctgcc gctggtgttc accctcgccg
     1321 gggtcggcat cgtcgccaac atgttcgtcg ccgatacccg caacgcgctg atcggctcgg
     1381 cgatcatcac ggccggcgtg ccggtgtacc tcgcctggcg gtggtggcgc ggccgccacg
     1441 atgcgcgatg acgatcagcg cgccggcgca tcgccggcga agaacgcaag gagcggcggc
     1501 gcgagaacct cgggcacgag atcgtgggtc gcgctctcga ggatcagcgc gcggctgtgg
     1561 ggcaccacac gcgccagcgc ctcggcggtc tcgcgcagga acgggtgggc ggcgccggcg
     1621 atcgccaggg tcgggcaggg gacgcggcgt gcgcgagcca ccggcagacg gccgtcgccg
     1681 acgatcgtgg cgtcgtacac cagcgtgtga gccatcgcct cgagctgcgg gcgaaacggc
     1741 gcgtggcgca gctgggcgac catcggctcg gggagcccga ccatgcggag ctggaagtac
     1801 tcgaccgcct cgccgcggcg gccggcg
//
LOCUS       DASVOR010000664         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_386118,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000664 DASVOR010000000
VERSION     DASVOR010000664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386118"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1932)
                     /locus_tag="VF516_13120"
     CDS             complement(<1..1932)
                     /locus_tag="VF516_13120"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8108666.1"
                     /translation="MAVVDTEQPAAEDAAKPIIVCVDDELNNLRALARVLGGKFTVLL
                     ANNGLEALDHVQKYPEVACVLADLRMPGLPGAELLARVAELRPLCRRAVVTGFPESED
                     LIKAINDGHLHYVITKPWKLSDLLQVVEQLVHTFRLERDNARLVAELRLANQHLREHE
                     RVLVAQLESRGREISAATEQLAQMGIQLDALTLRDGLTGLYTHRAFQERLREECARAL
                     RYGQPVSLMIADIDAFASVNYDLGYQIGDDILRRVALVLQEDESPDRPRGSDIVARYS
                     GEEFVLLMPETGKAGALTKAARLRDAVAVADMPGGRHMSVSIGVASLPDDAADPEGLL
                     TAAEAAVRGAKRGGPGRVHFFSAGDDPSLARSASRQFTPLPQLDRFRPYQERMNEVTA
                     VLMRDRSLSCLLVDLSRLHRVELDLGVAHHSEIYDHAAEVLDRLRSTVLDRSDLICRS
                     GDGDGYLVILSPRPGQRVDLEGLAFAVERAVEDGLAPMVSEVLREQPRITVGSARVLG
                     NSLLRPERIAARLVTDAVAATRNARERKAHRDRSTLQDIILGDGLTSFYQPIVDLGSG
                     DIFAYEALTRGPRGTALESPATLFAIADEVDLTVELDRACFRGALRNALTLEPVHRLF
                     VNLLPMSFYDAAFIEVEVGN"
     gene            complement(2032..>3027)
                     /locus_tag="VF516_13125"
     CDS             complement(2032..>3027)
                     /locus_tag="VF516_13125"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX8108667.1"
                     /translation="PDARPTVAEAAVPAVAAYVAGWLALHPDTGLDAGRDADSSAGLG
                     APCLASRPAAAADADARFVVVRWQVVCAADLARIALDFRAFFAVDARHEAIVTVHARD
                     APGEAVVVRAADPILRVQAGEAIGLAGWIAAGVDHIWEGRDHVSFVLALLLVVMLVRS
                     GTGWDTRPPVATLRRTATIITAFTVAHSLSLIAASLGWVRLPSRLVESLIAVSILYTA
                     IENIVRPDVRWRFVLTFGFGLVHGLGFASVLAVMLPPDHVIAPLLGFNVGVEVGQLVI
                     VVIALPLAWLAARELGAGRYRRGAMPVLSVALAALAVKWLIERVFAVSLLTLWGM"
BASE COUNT          474 a         1066 c         1099 g          388 t
ORIGIN      
        1 gttgccgacc tcgacctcga tgaacgcggc gtcgtagaac gacatcggca acaggttgac
       61 gaacagccgg tggaccggct cgagggtcaa cgcgttgcgc agcgcgcccc ggaagcacgc
      121 gcggtcgagc tcgacggtga ggtcgacctc gtcggcgatc gcgaacaggg tcgccggcga
      181 ttcgagcgcg gtgccgcgcg ggccgcgggt cagcgcctcg taggcgaaga tgtcgccgct
      241 gccgaggtcg acgatcggct ggtagaacga ggtcagcccg tcgccgagga tgatgtcctg
      301 cagggtcgag cgatcgcggt gggccttgcg ctcgcgcgcg ttgcgggtcg cggcgaccgc
      361 gtcggtgacc agccgggcgg cgatccgctc gggccgcagc agcgagttgc cgagcacgcg
      421 ggccgagccg accgtgatcc gcggctgctc gcgcagcacc tcgctgacca tcggcgccag
      481 gccgtcctcg accgcgcgct cgacggcgaa cgccaggccc tcgaggtcga cccgctggcc
      541 gggccgcggc gacaggatca cgaggtagcc gtcgccgtcg cccgaccggc agatcaggtc
      601 ggagcggtcg agcaccgtgc tgcgcagccg gtcgagcacc tcggcggcgt ggtcgtagat
      661 ctcgctgtgg tgggcgaccc cgaggtcgag ctcgacccgg tgcagccgcg acaggtcgac
      721 cagcaggcac gacagcgagc ggtcgcgcat caggaccgcg gtgacctcgt tcatccgctc
      781 ctggtagggc cggaaccggt cgagctgggg cagcggggtg aactggcggc tcgccgaacg
      841 cgccagcgac gggtcgtcgc cggccgagaa gaagtggacg cggcccggcc cgccgcgctt
      901 ggcgccgcgc accgcggcct cggcggcggt cagcaggccc tcggggtcgg ccgcgtcgtc
      961 gggcaacgac gcgacgccga tcgacaccga catgtggcgg ccgcccggca tgtcggcgac
     1021 cgcgaccgcg tcgcgcagcc gcgccgcctt ggtcagcgcg ccggccttgc cggtctccgg
     1081 catcagcagc acgaactcct cgccggagta gcgcgcgacg atgtccgagc cgcgcggccg
     1141 atccggcgac tcgtcctcct gcaggaccag cgcgacccgg cgcaggatgt cgtcgccgat
     1201 ctggtagccg aggtcgtagt tgacgctggc gaacgcatcg atgtcggcga tcatcagcga
     1261 caccggctgg ccgtagcgca gcgcccgcgc gcactcctcg cgcagccgct cctggaacgc
     1321 gcggtgggtg tacaggccgg tcagtccatc gcgcagcgtg agcgcgtcga gctggatgcc
     1381 catctgggcc agctgctcgg tcgccgccga gatctcgcgg ccgcgcgact ccagctgggc
     1441 gaccagcacg cgctcgtgct cgcggaggtg ctggttggcg agccggagct cggcgaccag
     1501 tcgggcgttg tcgcgctcca gccgaaatgt atgaaccagc tgttcgacca cctggaggag
     1561 gtcagagagc ttccacggct tggtgatgac gtaatgcagg tggccatcgt tgatggcctt
     1621 gatcaggtcc tccgactcgg ggaaccccgt gaccaccgcg cggcggcaca gcggccggag
     1681 ctcggcgacc cgcgccaaga gctcggcgcc cggcagcccc ggcatgcgca agtccgcaag
     1741 cacacaggcg acctcggggt acttctggac gtggtccagc gcctccaggc cgttgttggc
     1801 caggagcacc gtgaattttc caccgagcac ccgggcgagc gccctcagat tattgagctc
     1861 gtcatcgacg cagacgatga tcggcttggc cgcgtcctcg gctgctggct gttcggtgtc
     1921 aacgacggcc aacgtgcagg aagtatcgac cttgccgttc ccactgtgaa gagcttcgcg
     1981 cggcgagtta cccgactcta accccgctca cccagcttgc cgccgatcgg cctacatgcc
     2041 ccacagcgtc aacagtgaga ccgcaaatac ccgctcgatc agccatttga cggcgagcgc
     2101 ggcgagcgcg acggacagca ccggcatcgc gccgcgccgg tagcgcccgg cgccgagctc
     2161 gcgggcggcg agccaggcca gcggcagcgc gatcaccacg atcacgagct ggccgacctc
     2221 gacgccgacg ttgaacccca gcagcggcgc gatcacatgg tcgggcggca gcatcaccgc
     2281 gagcacgctg gcgaatccca ggccgtgcac caggccgaac ccgaaggtca ggacgaaccg
     2341 ccagcgcacg tccgggcgga cgatgttctc gatcgcggtg tacaggatcg acacggcgat
     2401 cagcgattcc acgagccggc tcggcagccg gacccagccg agcgacgccg cgatcagcga
     2461 caggctgtgg gcaacggtga acgcggtgat gatcgtggcc gtgcggcgca gcgtggcgac
     2521 cggcggccgg gtgtcccagc cggttccgct gcgcacgagc atcaccacga gcaggagcgc
     2581 gagcacgaag ctgacgtgat cgcggccctc ccagatgtgg tcgacgccgg cggcgatcca
     2641 gccggcgagt ccgatcgcct ccccggcctg gacgcgcagg atcggatcgg ccgcccggac
     2701 cacgacggct tcgccgggtg catcgcgggc gtggacggtg acgatcgctt catggcgcgc
     2761 atcgacggca aagaatgccc ggaagtcgag cgcgatccgc gcgaggtcgg cggcgcatac
     2821 gacctgccag cggaccacga cgaaccgcgc gtcggcgtcg gcggctgcag cgggccggct
     2881 cgccagacac ggcgcaccca ggccggcgct cgaatcggcg tcgcggccgg cgtcaaggcc
     2941 ggtatcagga tggagcgcga gccagcccgc gacgtacgcg gcgaccgcgg ggaccgccgc
     3001 ctcggcgacc gtcggccgcg cgtcggg
//
LOCUS       DASVOR010000665         4867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_357060,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000665 DASVOR010000000
VERSION     DASVOR010000665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357060"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="VF516_13130"
     CDS             <1..1310
                     /locus_tag="VF516_13130"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8108668.1"
                     /translation="RDRRWLRTFVARLTERSGGLRKLWDRSEHDIYGAPGAPRQHQLP
                     NNRFAVQSDGTSIWLTGEGSSDAGDRPFLDRLDLATLRTERLFRADRQSFERVEYVYP
                     AARRSFLTIKESPRDPPNLALRTLGGAVAAPAAGEPSLASDVKMLTGFPDPTPQIRGV
                     SKQIVTTRRADGAQVSFTLYLPPGHRPGTRLPTVFWAYPLEFSDPTTAGQVAARPSAF
                     TQPRGASPVLYALAGYAVLEVSMPVIGPPETVYDSFVEQIVSNARAAIDKAVELGVTD
                     RDRVGVSGHSHGALMTANLLAYSNLFRAGVARSGAYNHTLRPFGFQTEHRTFYTARDT
                     YLKLSPLINAEKIDEPLLLIHGQLDVNPGTVPLQSEKLFEALRGLGATARLVMLPFES
                     HGYEALESIETVMAESVDWFDRYVKNAPPRKAVTSVEPVRPSK"
     gene            1366..2241
                     /gene="tesB"
                     /locus_tag="VF516_13135"
     CDS             1366..2241
                     /gene="tesB"
                     /locus_tag="VF516_13135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HEX8108669.1"
                     /translation="MKGILADLVSLLGLERLDRDLFRGRSQDLGWGAIFGGQVVGQAL
                     SAAAQTVGSGRIVHSLHGYFMRAGDVRGSIIYQIDRLRDGKSFTTRRVVAVQEGEAIF
                     SLEASFQSDEDGLDHQDAMPEVPAPETLRSEREIGLDLAAELPPHLLARATAERPIEI
                     RPVEMRDPRHPVAVPARRRAWYRAIDQLPDEPALHRYLLAYASDFAFLGTALDPHAMS
                     WLTPGLQMASLDHVMWFHRPFRLDDWLLYVVDSPSASGARGLVRGQFFDRAGRLVAST
                     AQEGLIRRHRPTHVP"
     gene            2342..2857
                     /locus_tag="VF516_13140"
     CDS             2342..2857
                     /locus_tag="VF516_13140"
                     /inference="COORDINATES: protein motif:HMM:NF025116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8108670.1"
                     /translation="MRTTLVLTVLAPDRTGLVELLAQRIAAAGGNWEESRMARLAGQF
                     AGILLVTCDTARSDELAAAMRTLESAGLQVTARVAQADPAPAPGPRIQLALTGSDRAG
                     IIRDVSRVLAERGVNVEELESEVSSAPMSGEPLFTARARLRVPPTLSLADLRASLEML
                     AGELMVDLGAE"
     gene            complement(2882..4570)
                     /locus_tag="VF516_13145"
     CDS             complement(2882..4570)
                     /locus_tag="VF516_13145"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8108671.1"
                     /translation="MAIDVTSLANRRATDRWNRVAVGDMLERLTWSYPDQDALVGWPG
                     AFAYPEHARLSYRRANALANRIANALLAAGCAGKDRVLVVCENSVEAVIIKLGVQKAG
                     LVVAPVNPSLAADVVEDVIQRIAPRLAIVDAEVWPRVEAAFARAGMAVAVTLPIGGDA
                     IPGSRGLREFVADAPDTEPDVEIHGDDICELQFTSGTTAMPKAVMLSHTNAYFAASGY
                     ALTLTRGVRCESELRLCSFLPLVYHIGGQVFGLPALLSGGTLVIGRRPVADQVADCVV
                     RERITALWAGSPIMVQAWIEAMQRAQLTAPQLAVIAYGWAAISPAQLAAITRLTSDRV
                     VALEVFGQTEAIACYRFWPHRWPEVFAAHCPESNYVGVPSPLLASHLMAPDGSSLEGK
                     PGVLGEAVYRSPTVMQGYYQDEAATRAAFAHGWFHSGDSCVYDPAGLRIMVDRLKDIV
                     KTGGENVSSLRVESVLVQHPGVLRAAVIGLPNPRWGEAVTAVVMRKPGTEAGIEAGIE
                     AGIDPVSEPDLIAFCRARLAGFETPKRVVFVDRYPETVGGKVLKHVLRAELGGR"
     gene            4685..>4867
                     /locus_tag="VF516_13150"
     CDS             4685..>4867
                     /locus_tag="VF516_13150"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8108672.1"
                     /translation="MAWTLDPAQREILDAADRFARRELYPLAARMDAEEWWPDGVMAR
                     LAAAGLLGITIPGELGG"
BASE COUNT          684 a         1787 c         1722 g          674 t
ORIGIN      
        1 agcgcgatcg ccgctggctg cggacgttcg tcgccaggct caccgagcgc tccggcggcc
       61 tccgcaagct gtgggaccgc agcgagcacg acatctatgg cgctcccgga gcgccgcggc
      121 agcatcagct gcccaacaac cggttcgccg tgcagagcga tgggacgtcg atctggctga
      181 ccggcgaggg ctcgtccgac gccggcgacc ggccgttcct cgatcgcctc gacctcgcca
      241 cgctccgcac cgagcggctg ttccgcgccg atcgccagag cttcgagcgc gtcgagtacg
      301 tgtacccggc cgccaggcgc agcttcctca ccatcaagga gtcgccccgg gacccgccca
      361 acctcgcgct gcgcacgctc ggcggcgcgg tcgccgcgcc cgcggccggc gagcccagcc
      421 tggccagcga cgtgaagatg ctgaccgggt ttcccgaccc gacgccgcag atccgcgggg
      481 tgtccaagca gatcgtgacg accaggcgcg ccgacggtgc gcaggtctcg ttcacgctgt
      541 acctgccgcc gggccaccgg ccgggcacgc ggctgccgac ggtgttctgg gcctatccgc
      601 tcgagttctc cgacccgacg accgcgggcc aggtcgccgc ccggcccagc gcgttcaccc
      661 agccgcgcgg cgcctcgccc gtgctgtacg cgctcgccgg gtacgccgtg ctcgaggtgt
      721 cgatgccggt gatcggcccg ccggagacgg tctacgacag cttcgtcgag cagatcgtct
      781 ccaacgccag ggcggcgatc gacaaggcgg tcgagctcgg cgtcaccgac cgcgaccgcg
      841 tcggcgtgtc cggccacagc cacggcgcgc tgatgacggc gaacctgctg gcgtactcga
      901 acctgttccg cgccggggtg gcgcgcagcg gcgcgtacaa ccacacgctg cggccgttcg
      961 gcttccagac cgagcaccgc acgttctaca ccgcgcgcga cacctacctg aagctgtcgc
     1021 cgctgatcaa cgccgagaag atcgacgagc cgctgctgct gatccacggc cagctcgatg
     1081 tcaacccggg caccgtgccg ctgcagtccg agaagctgtt cgaggcgctg cgcggcctcg
     1141 gcgccaccgc gcgcctggtg atgctgccat tcgaatcgca cggctacgag gcgctcgagt
     1201 cgatcgagac cgtgatggcg gagtcggtcg actggttcga tcgctacgtg aagaacgccc
     1261 cgccgcgcaa ggcggtgacc tcggtcgagc cggtgcggcc gagcaagtga tcggtgctgc
     1321 gccgcggacg aactcaagcc gggcgcgccg tgctacgagc tgtgcatgaa gggcatcctc
     1381 gccgacctgg tatccctcct gggcctcgaa cgcctcgatc gcgacctgtt ccgcggacgc
     1441 agccaggatc tcggctgggg tgcgatcttc ggcgggcagg tggtcggaca ggcgctgtcc
     1501 gcggcggccc agaccgtcgg cagcgggcgg atcgtccact cgctgcacgg ctacttcatg
     1561 cgcgccggcg atgtgcgcgg ctcgatcatc tatcagatcg atcggctgcg cgatggcaag
     1621 agcttcacca cccggcgcgt cgtcgcggtc caggagggcg aggcgatctt cagcctcgag
     1681 gcatcgttcc agtccgacga ggacgggctc gaccaccagg acgcgatgcc cgaggtgccg
     1741 gcgcccgaga cgctgcgctc cgagcgcgag atcgggctcg acctggccgc ggagctcccg
     1801 ccgcacctgc tggcgcgcgc caccgccgag cgtccgatcg agatccgccc ggtcgagatg
     1861 cgcgacccgc ggcacccggt cgcggtcccg gcgcgccgcc gggcgtggta ccgcgcaatc
     1921 gaccagctcc ccgacgagcc agcgctgcac cgctacctgc tcgcctacgc gtcggacttc
     1981 gcgttcctgg gcactgcgct cgatccgcac gcgatgagct ggctgactcc cggcctgcag
     2041 atggcgtcgc tcgatcacgt gatgtggttc catcgcccgt tccggctcga cgactggctg
     2101 ctctacgtgg tcgatagccc gtcggcgtcg ggcgcccgcg gcctggtgcg cgggcagttc
     2161 ttcgaccgcg ccggacggct cgtcgccagc accgcgcagg aggggctgat ccggcggcac
     2221 cgcccgaccc acgtgccctg accgcggtgc cgcgcccggc agcaggcgcc gacgtacgac
     2281 gaggtcggcg aggacggcgc gggaccgctc ccgggcgcgc gcggccatgg caagcttgac
     2341 catgcgcacc acgctggtcc tcaccgttct cgcgcccgat cgcacgggcc tggtcgagct
     2401 gctcgcgcag cggatcgccg cggccggcgg caactgggag gagagccgca tggcgcggct
     2461 cgccggtcag ttcgccggca tcctgctcgt gacgtgcgac accgcgcgca gcgacgagct
     2521 ggccgcggcg atgcgcacgc tcgagtccgc cgggctgcag gtgacggcgc gcgtcgcgca
     2581 ggccgacccg gcacctgcgc ccgggccgcg gatccagctc gcgctgaccg gctccgaccg
     2641 cgccggcatc atccgcgatg tctcgcgcgt cctcgccgag cgcggggtca acgtcgagga
     2701 gctcgagtcg gaggtgtcga gcgcgccgat gtccggcgag ccgctgttca ccgcccgcgc
     2761 gcggctgcgc gtgccgccga ccctctcgct cgccgatctg cgcgcgtcgc tcgagatgct
     2821 ggccggcgag ctgatggtcg atctcggcgc cgagtagccg gcgaacaccg cgccccggcg
     2881 gtcagcggcc gccgagctcg gcgcgcagca cgtgcttgag caccttgccg ccgacggtct
     2941 cggggtaccg gtcgacgaac accacgcgct tgggggtctc gaacccggcg agccgcgcgc
     3001 ggcagaacgc gatcaggtcg ggctcggaga ccgggtcgat tcccgcctcg attcccgcct
     3061 cgattcccgc ctcggtgccc ggcttgcgca tcacgacggc ggtgaccgcc tcgccccacc
     3121 gcggattggg caggccgatc acggcggcgc gcagcacgcc ggggtgctgg acgagcaccg
     3181 actcgacccg caggctcgac acgttctcgc cgccggtctt cacgatgtcc ttcaggcgat
     3241 cgaccatgat gcgcaggccg gccgggtcgt agacgcagct gtcgccggag tggaaccagc
     3301 cgtgcgcgaa cgctgcgcgc gtcgcggcct cgtcctggta gtagccctgc atcacggtcg
     3361 gcgagcggta cacggcctcg ccgagcacgc cgggcttgcc ctcgagcgag ctgccgtcgg
     3421 gcgccatcag gtgcgaggcg agcagcgggc tcggcacgcc gacgtagttg ctctccgggc
     3481 agtgcgccgc gaacacctcg ggccagcgat gcggccagaa ccggtagcag gcgatcgcct
     3541 cggtctggcc gaacacctcg agggcgacga cgcggtcgct ggtgagccgg gtgatcgcgg
     3601 cgagctgcgc cggcgagatc gcggcccagc cgtaggcgat cacggcgagc tggggcgcgg
     3661 tcagctgcgc gcgctgcatg gcctcgatcc aggcctggac catgatcggc gagccggccc
     3721 acagcgcggt gatccgctcg cgcaccacac agtcggcgac ctgatcggcg accggccggc
     3781 ggccgatcac gagcgtgccc cccgacagca gcgcgggcag cccgaacacc tggccgccga
     3841 tgtgatagac cagcggcagg aacgagcaca gccgcagctc gctctcgcag cgcacgccgc
     3901 gggtcagcgt gagcgcgtag cccgacgccg cgaagtacgc gtttgtgtgc gacagcatca
     3961 cggccttcgg catcgcggtc gtgccggacg tgaactgcag ctcgcagatg tcatcgccgt
     4021 ggatctcgac atccggctcg gtgtcgggcg cgtccgcgac gaattcgcgc agtccgcgcg
     4081 agccggggat tgcgtcgccg ccgatcggga gggtgaccgc gaccgccatg ccggcgcggg
     4141 cgaacgcggc ctcgaccctg ggccagacct cggcgtcgac gatggcgagc cggggcgcga
     4201 tgcgctggat cacgtcctcg acgacgtcgg ccgcgagcga cgggttgacc ggcgcgacca
     4261 ccagtccggc cttctgcacg ccgagcttga tgatcaccgc ctccaccgag ttctcgcaca
     4321 ccaccagcac gcggtccttg cccgcgcagc cggcggcgag cagtgcgttg gcgatccggt
     4381 tggcgagcgc gttggcgcgg cggtagctga gccgcgcgtg ctcgggatac gcgaacgcgc
     4441 cgggccagcc gaccagcgcg tcctgatccg ggtagctcca ggtcaggcgc tcgagcatgt
     4501 cgccgaccgc gacgcggttc cagcgatcgg tggcgcgacg gttcgcgagg ctggtgacgt
     4561 cgatcgccat gcgtctactg agtcatactt agcaagtccg atcaagcgca aagtcgaact
     4621 cgagcatatt cgacgacgtg tgtcggatat agacaaatga gacgatctca gcaacactcg
     4681 ccgcgtggcc tggacgctcg acccggcgca gcgcgagatc ctcgatgccg cggaccgctt
     4741 cgcgcgccgc gagctctacc cgctcgccgc ccggatggac gccgaggagt ggtggccgga
     4801 cggcgtgatg gcccggctcg cggccgccgg cctgctcggg atcacgatcc ccggcgagct
     4861 cggtggc
//
LOCUS       DASVOR010000666         4208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_240061,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000666 DASVOR010000000
VERSION     DASVOR010000666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240061"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VF516_13155"
     CDS             complement(<1..462)
                     /locus_tag="VF516_13155"
                     /inference="COORDINATES: protein motif:HMM:NF014034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108673.1"
                     /translation="MSALPGRLEFRDPVRGAALTEALANRARSLGRAPVSVMHVCGSH
                     EQAIARFGLRAAFPAELDVIMGPGCPVCVTDVPEVDEAVALARQGVRIATYGDMLRVP
                     GTAGSLADERARGARVDVVYGVAQAAELARSGEPVVFFASGFETTAVATAAV"
     gene            complement(459..743)
                     /locus_tag="VF516_13160"
     CDS             complement(459..743)
                     /locus_tag="VF516_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HEX8108674.1"
                     /translation="MCLGVPGRVISIDGDIALVDFWGVTKPVRLDVVDAPVAPGDYVL
                     NHVGFAIRRIPPEEIGETLALYDELLRRAGADDLMAADVRGELDATGKRS"
     gene            complement(1066..1479)
                     /locus_tag="VF516_13165"
     CDS             complement(1066..1479)
                     /locus_tag="VF516_13165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108675.1"
                     /translation="MSDVHQVDAGDAPAERDLDQHWKRPPEGMAPGRSGAWWGRANAE
                     PKIGLDQRGEAMTALARRLVATGMSRAEFAFGEVCDLDRGVIDALHAGPTQLPAAGGR
                     RRRRALTTRGRAEAPGLAGALGARRCGYRSQRGDG"
     gene            complement(1619..2335)
                     /locus_tag="VF516_13170"
     CDS             complement(1619..2335)
                     /locus_tag="VF516_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108676.1"
                     /translation="MLHNKDALSTSVRQAHEPSAPYPTSSNAEKYLVLRALFVVSLAL
                     LVGGGIPALLAYNQGVFFQQDEIIIICWLIASIWTQWLSHYRLTCTEFDSHGYVAWSE
                     ALHHKTTERLSDTFSRWQLSSLLLVSAFSSFFVFYWRNIHDRGDSRYLPSALSIQVIG
                     IVSWIIISLPLIATWRHWHGLRACAIHELATSSEEVARIDLRNRMIEAVKPIGSWNFL
                     VSSSLLLVSFLSPLIKLMIH"
     gene            2838..3860
                     /locus_tag="VF516_13175"
     CDS             2838..3860
                     /locus_tag="VF516_13175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEX8108677.1"
                     /translation="MFKAVFGQPEHARGVLRAIVPAALAEALEWSTLTPQPGSFVDAA
                     FQQQHTDLLYAVTWRDGGDALVYLLFEHQSALPTEGLMAERLLGYQVRIWDRWRTNHP
                     RAKTLPMILPIVMYHGASFWSEPRSFDELLEVPAALRSAAEPYLVRFSYLLHDLSGVS
                     DEELRDGAMRTALAKLVAVCFKYARTRTDFVRILGRWMDVAREVLRAPHGLEALAQVM
                     RYILEVNDHVEPEELQELLERDLGPEAKDAIMTAGQRLIEQGRQQGIEQGRQQGIEQG
                     RQQGIEQGRQQGIKGVLLRLLRQRFGDAVNAQVEQRIATASLEQVEAWTGRVLSAATL
                     AEAIAD"
     gene            3899..4114
                     /locus_tag="VF516_13180"
     CDS             3899..4114
                     /locus_tag="VF516_13180"
                     /inference="COORDINATES: protein motif:HMM:NF025619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HEX8108678.1"
                     /translation="MTAGQRLIEQGRQKGRQQGIQEFLLRLLRQRFGDAVDAHVEQRI
                     ATASIEQIETWPSRVLSAATLAELIAD"
BASE COUNT          769 a         1404 c         1340 g          695 t
ORIGIN      
        1 caccgccgcg gtcgcgaccg cggtcgtctc gaaccccgag gcgaagaaca ccaccggctc
       61 gccggagcgc gccagctcgg ccgcctgcgc gacgccgtag accacgtcga cccgggcgcc
      121 ccgcgcacgc tcgtcggcga gcgagcccgc cgtgccgggg acccgcagca tgtcgccata
      181 cgtcgcgatc cgcaccccct ggcgcgcgag cgcgaccgcc tcgtcgacct cgggcacgtc
      241 ggtgacgcac accgggcacc ccggtcccat gatcacgtcg agctcggccg ggaacgcggc
      301 gcgcagcccg aacctggcga tcgcctgctc gtggctgcca cacacgtgca tcaccgacac
      361 aggagcgcgc cccaggctcc gagcgcggtt cgcgagcgcc tcggtcagcg ccgccccgcg
      421 caccggatcg cggaactcga gtctgcccgg aagcgcgctc acgaccgctt ccccgtcgcg
      481 tcgagctcgc cccgcacgtc ggccgccatc aggtcatcgg cccccgcgcg ccgcagcagc
      541 tcgtcgtaca gcgccagcgt ctcgccgatc tcctcgggcg ggatccgccg gatcgcgaag
      601 ccgacgtggt tgagcacgta gtcgccggga gcgaccggcg cgtcgaccac gtcgagccgg
      661 accggcttgg tcacgcccca gaagtcgacg agcgcgatgt cgccgtcgat cgagatcacg
      721 cggccgggaa cgccgagaca catgctgcgg acctttgcat ccgacgtgcc gcgcgtcgac
      781 cgcgcggcgc gcctccgatt gcgcgaaaac cgacggcggc ctcgggcgat cccgcaaacg
      841 ctgcgcgatc tgcgcagagg ctcagcgcca cgcgagcgcg tcggcgacca ccgcctggcc
      901 gagcgcgatt ctgccgtcgc caaggaggca cgtcggcgtg gaggcggacg tcgaggtcac
      961 gcagggcgca ccagccctgg gcagccgtgg ggttctgaaa cgcaccgccg gcagctcgct
     1021 cctcaccagc cgcgccgatc gcatgcgtta ggctgcttcc cgcccctatc catcgccgcg
     1081 ctgcgatctg tacccgcagc gtcgcgctcc cagagcccct gcaagccctg gcgcttcggc
     1141 gcgacctcgc gtggtcagcg cccgccgccg gcggcgtcca ccagccgcag gcagctgcgt
     1201 cgggccggca tgcagcgcgt cgatcacccc gcggtcgaga tcgcagacct caccaaacgc
     1261 gaactcggcg cgcgacatgc cggtggccac gagccgtcgt gccagcgcgg tcatcgcctc
     1321 gccgcgctga tccaggccga tctttggctc ggcattcgct cggccccacc aagccccgct
     1381 gcgaccgggc gccatcccct cggggggccg cttccaatgc tggtcgaggt cgcgctcggc
     1441 gggcgcatcg ccggcgtcga cttggtggac gtcgctcaag gcaggctggc gcagcgcggt
     1501 ccgcgagcag atgccgtacc atcgcagcaa ccgactgaca cgagcttccg tggttccgtg
     1561 gtctcttgtc gtgcgaacat atacaacgaa tggcgaatcg ccctggctgc ctaatattct
     1621 aatgaatcat cagctttata aggggactaa ggaatgaaac tagcagaaga gaggatgaaa
     1681 caagaaaatt ccatgatcca atcggtttta cagcttcaat cattcggttt cgtagatcga
     1741 ttctggcaac ttcctctgac gaagtagcca attcatgaat ggcacacgca cgcaggccat
     1801 gccaatgtcg ccatgttgca atcaaaggaa gagaaataat gatccaggat acgattccaa
     1861 tgacctgaat tgaaagtgct gagggcagat atctgctatc gcctctatcg tggatatttc
     1921 tccaataaaa gacaaagaat gatgagaatg ccgataccaa gagaagagac gatagctgcc
     1981 aacgagaaaa tgtatccgag agacgttccg ttgtcttatg atgcagcgcc tccgaccatg
     2041 cgacataacc atgtgagtcg aattctgtgc aggtgaggcg gtaatgggat agccattgtg
     2101 tccatatcga tgcgattagc caacaaatta taatgatctc atcctgctga aaaaacacac
     2161 cctggttgta ggccaacagc gctggaatcc ctccgccgac caatagagcg agactcacaa
     2221 caaaaagagc tcgaagtacc agatatttct cagcattgct ggaagttggg taaggtgccg
     2281 acggttcatg agcttggcgt actgacgtcg aaagcgcgtc cttgttgtgc agcatatggc
     2341 gatagtgtta gcgtgtagtg tcctatggta ggttaccgga gatattacgt ctagtgaaag
     2401 cttaattcat cgggatatgc ctactcctta ttgtgggctt ctgccgagct gcggagcagt
     2461 tggcatagct gccaatttcg cgacgcgacc tgtcgaccgg catcgcacct gcgccggccg
     2521 ccgttcgatc gcaggtagcg tccctcgccg ccgacacgca gccgcccccc ctcgcggcgg
     2581 ctggccaggg gaagtccggc gctttcgttc gcggccgtcg atgcggatcg ttgcggcccc
     2641 acagtcattg gcgtcggccg gagcgagctc ggcgcgctgg gccgtagcgc tgagcgcagc
     2701 cgaagcgtcg tcgattgcac gacgtcgaag ctgccgagat cggccggacc gtgcggaacc
     2761 ggtgcccgtc gagcttgcct tggtcgcacc gcgctcctac ggtggcgtcg gggatgattc
     2821 cgaacccgca cgacttgatg ttcaaggccg tattcggcca gcccgagcat gcccgcggag
     2881 tgctgcgcgc gatcgtgccg gcggcgctgg ccgaagcgct ggagtggtcg acgttgacgc
     2941 cgcagccagg cagcttcgtc gatgccgcct tccagcagca gcatacggac ctcctgtacg
     3001 cggtgacgtg gcgcgatggc ggcgacgcgc tcgtgtacct gctgttcgag caccagtccg
     3061 cgctcccgac cgagggcctg atggccgagc gcctgctcgg ttatcaggtc cggatctggg
     3121 accgctggcg cacgaatcac ccgcgggcca agacgctgcc gatgatcctg ccgatcgtga
     3181 tgtaccacgg cgcctcgttc tggtcggagc cccggtcgtt cgacgaactg ctcgaggtac
     3241 ccgctgcgct gcggtccgcg gcggagccgt acctggtccg gttcagctac ttgctccacg
     3301 atctctcggg ggtctccgac gaggagctgc gagacggcgc gatgcgaacc gcgctcgcga
     3361 agctggtcgc cgtgtgcttc aagtacgcgc ggacccgcac ggactttgtc cggatcctcg
     3421 gccgctggat ggacgttgcg cgcgaggtgc tccgagctcc gcatggactg gaggcgctgg
     3481 cgcaggtgat gcgctatatt cttgaagtca acgaccacgt ggaacccgag gagctacagg
     3541 agcttctgga acgcgatctc ggacctgaag ccaaggacgc catcatgacc gccggacagc
     3601 gactgatcga acaaggacgc cagcagggca tcgaacaagg acgccagcag ggcatcgaac
     3661 aaggacgcca gcagggcatc gagcagggcc gccagcaggg catcaaggga gtgctcctgc
     3721 gtctcctgcg gcagcgcttc ggcgatgcgg tgaatgctca ggtcgagcaa cgcatcgcga
     3781 ccgcgtctct cgagcaagtc gaagcctgga ccgggcgcgt gctgtcggcc gccacgctcg
     3841 ccgaggcgat cgctgactga accctgggca gcagccgcgc aggatccgtg acgccatcat
     3901 gaccgccgga cagcgactga tcgaacaagg ccgccagaag ggccgtcagc agggtatcca
     3961 ggagttcctg ctgcgcctcc tgcggcagcg cttcggcgat gcggtggacg ctcatgtcga
     4021 gcaacgcatt gcgacggcct ccatcgagca gatcgagaca tggccctcgc gcgtgctctc
     4081 ggccgccacc ctcgcggaac tgattgccga ctgaacccgg gcctgccatg tcgtcctgca
     4141 tttgcggagc tgcccgagac cgcggggtgt agagctcacg atcgccgtgc gatcgcgtcg
     4201 gcgaccat
//
LOCUS       DASVOR010000667         4558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211046,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000667 DASVOR010000000
VERSION     DASVOR010000667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211046"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VF516_13185"
     CDS             <1..454
                     /locus_tag="VF516_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108679.1"
                     /translation="VDSATDQSKVAPSDAGERLARVVKKTVERTGYATSWPGPLPTQT
                     ELASSHSRAFIVASTVKKIDISKVSRLTQISCKVEIRVAPWSGVDGGEKWEANKAASA
                     SGSARATTGNSDREIAGGVRDCLEAVAEDVTTSQILPFLKRLATSSGS"
     gene            complement(455..955)
                     /locus_tag="VF516_13190"
     CDS             complement(455..955)
                     /locus_tag="VF516_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108680.1"
                     /translation="MLRALAMCLAMTTAGCGIADFDVEQPVPAQTIQGSGLPAPLAAI
                     FPLPLNLDISSKIKAKDTGPIDGVTLSSLTLDITSSGGDWSFVTSIDVFVSSTKSGST
                     LPRAKIASVTSPGAVRTLSFHVEGGVNLKPYIDEGSQVDSAGMGTQPTQNVTFDGKGV
                     FTVHPL"
     gene            complement(1075..2289)
                     /locus_tag="VF516_13195"
     CDS             complement(1075..2289)
                     /locus_tag="VF516_13195"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX8108681.1"
                     /translation="MEAASKDVAALAALAARRRRAKNAYLILAVIAAAVAVLWFGHHW
                     WTHGKQDTDDAQVEADVVPIAPRVGGVIKTARVHDNQMVKAGDVLFEIDPVDLDVEVA
                     RCEAELQAAQAQQSAAEAQVGVVQSSSTGGLSSARAALTGAGASVRSAEEAMRAAQAA
                     VERAKADLATAESDLKRQQDLFAQKAGTRHDVEHAQQARDVAKASLDAANAQLAMARD
                     QHNLAQARVAEAEGHVTQSTPVDQAVASAQASAKLAAARVTAAQVALDKAKLQRSYAT
                     VVAPAAGRISRLGAHAGQQVMAGQPLLMLVPVDSYVIANFKESQVGRMKAGDPVDIEI
                     DAYSGETFHGVVDTVSPATGARFSMIPPDNATGNFVKVVQRVPVKILWSSPPGVAMLP
                     GLSAEVTVHTGG"
     gene            complement(2295..3947)
                     /locus_tag="VF516_13200"
     CDS             complement(2295..3947)
                     /locus_tag="VF516_13200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8108682.1"
                     /translation="MTAAPPASGAGGLIPSHAPRQVGKWTVAISIALGSVMSTIDTSI
                     VNVAIPQIRGELGASLQEITWITTGYMIAMVLVMPLTGFLGSFFGQKRVYLVSLVVFT
                     IGSVMCGTARSLEMLVLWRIVQGFGGGALQPTQQAILRQTFPPAEQGMAMAVFSMVIM
                     VGPAVGPVLGGWITDNYSWPWIFFVNLPVGVLGIYLTMRNVIEPADVLAANRARAAVA
                     RKHLDWAGIALMAIAIGALQYVLEEGPQNDWFDSTAILVGTYIAAVGLCAFVIRELTA
                     TAPVVDLRMFRDPTFASGTVIGCLVFAMLMGSMFLLPVFTQEMMKFTATESGVVLLPR
                     TIAMMVASPIVGRLYNQIRPAWLCAFGMVMTALGCWMQADINLATSSADLRLPLAVTG
                     IGFAFLFVPLTTAALANVARHELAGAAGVNSFVRQVGGSIGLSIFANLFTNYSHRAAA
                     GLAENVSILRPEVAAQAMMTRSTIMSHGMGPDAASALTQQSIAGRTLLQGTVIGFDKT
                     FLLQTIAFVVVIPLLLFLRVKRTKAAQGPAPEAPREVAMHLE"
     gene            complement(3944..>4558)
                     /locus_tag="VF516_13205"
     CDS             complement(3944..>4558)
                     /locus_tag="VF516_13205"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8108683.1"
                     /translation="AARVASRAELRSEMRRTMRETVRLAILDAAEELIARHGLHDAAL
                     VQIARRAGVAVGTLYNYFTDRDALIRALFETRRATFRPRMLAAIDAGLDLPFEPRLRR
                     FVRDLFEAFESHRSFLKLAIENEHLKPSASTAPQDLAAGVRDILAAGVREGAIPAATD
                     LLALAITGTIKAIVVQRIQAGAELVRDADAVVDFVLGGARRALR"
BASE COUNT          684 a         1551 c         1703 g          620 t
ORIGIN      
        1 cgtcgacagc gccaccgatc agtccaaggt cgcgccgagc gatgccggcg agcggctcgc
       61 ccgggtcgtc aagaagaccg tcgagcgcac cggctacgcg accagctggc ccggcccgct
      121 gccgacccag accgagctcg cgtcgagcca ttcccgcgcg ttcatcgtcg catcgaccgt
      181 caagaagatc gacatcagca aggtcagccg cctgacccag atctcgtgca aggtcgagat
      241 ccgcgtcgcg ccgtggagcg gcgtcgacgg cggcgagaag tgggaggcca acaaggccgc
      301 ctccgccagc ggctcggcca gggccaccac cggcaactcc gatcgcgaga tcgccggcgg
      361 cgtgcgcgac tgcctcgagg ccgtcgccga ggacgtcacc accagccaga tcctgccgtt
      421 cctcaagcgc ctcgcgacct ccagcggcag ctgactacag cgggtggacg gtgaacacgc
      481 ccttgccgtc gaacgtcacg ttctgggtcg gctgggtacc catgccggcg gagtcgacct
      541 ggctgccctc gtcgatgtag ggcttgaggt tgacgccgcc ctcgacgtga aagctcagcg
      601 tgcggaccgc gcccggcgag gtcaccgagg cgatcttcgc ccggggcagc gtcgagcccg
      661 acttcgtgct ggacacgaac acgtcgatcg acgtcacgaa gctccagtcg ccgccgctcg
      721 acgtgatgtc gagggtcagc gacgacaggg tcacgccgtc gatcgggccg gtgtccttgg
      781 ccttgatctt cgacgagatg tcgaggttga gcggcagcgg gaagatcgcg gcgagcggcg
      841 ccggaaggcc cgagccctgg atcgtctggg ccgggaccgg ctgctcgacg tcgaagtcgg
      901 cgatgccgca accggcggtg gtcatcgcca ggcacatagc gagcgcgcgc agcatgggct
      961 ccgagcatac gccggggcac gcgcggccgc ggcgaccgcg cgcccggatc acgccgcgat
     1021 cacgccgcgc gctggcgcac agggtggtgt ggtggccggc cggatcgagg cggctcagcc
     1081 gccggtgtgg acggtgacct cggcggacag gccggggagc atcgcgacgc cgggcggcga
     1141 cgaccacagg atcttgaccg gcacgcgctg gacgaccttg acgaagttgc cggtcgcgtt
     1201 gtcgggcggg atcatcgaga accgcgcgcc ggtggcgggc gacacggtgt cgaccacgcc
     1261 gtggaacgtc tcgccggagt aggcgtcgat ctcgatgtcg accggatcgc cggccttcat
     1321 ccggccgacc tggctctcct tgaagttggc gatcacgtag ctgtcgaccg ggaccagcat
     1381 gagcagcggc tggcccgcca tgacctgctg gccggcgtgc gcgccgagcc tggagatcct
     1441 gccggcggcc ggggcgacga cggtggcgta gctgcgctgg agcttggcct tgtcgagcgc
     1501 gacctgggcg gcggtgacgc gcgcggcggc gagcttggcg ctggcctggg cggaggcgac
     1561 cgcctggtcg accggcgtgg actgggtgac gtggccctcg gcctcggcga cgcgggcctg
     1621 ggcgaggttg tgctgatcgc gcgccatcgc gagctgggcg ttggcggcgt cgagcgaggc
     1681 cttggcgacg tcgcgcgcct gctgggcgtg ctcgacgtcg tggcgggtgc ccgccttctg
     1741 ggcgaacagg tcctgctggc gcttgagatc ggactcggcg gtcgcgaggt cggccttggc
     1801 gcgctcgacc gcggcctggg cggcgcgcat cgcctcctcc gcgctgcgca ccgaggcgcc
     1861 ggctccggtc agcgccgcgc gcgccgacga caggccgccg gtcgacgacg actggacgac
     1921 gccgacctgg gcctcggccg cggactgctg ggcctgggcg gcctggagct cggcctcgca
     1981 gcgcgcgacc tcgacgtcga ggtccaccgg atcgatctcg aacagcacgt cgccggcctt
     2041 gaccatctgg ttgtcgtgga cccgcgcggt cttgatcacg ccgccgaccc gcggcgcgat
     2101 cggcacgacg tcggcctcga cctgcgcgtc gtcggtgtcc tgcttgccat gcgtccacca
     2161 gtggtggccg aaccacagca cggcgacggc cgcggcgatc accgcgagga tcaggtaggc
     2221 gttcttggcg cggcggcggc gggcggcgag ggcggcgagg gcggcgacgt ctttgcttgc
     2281 ggcttccatg gcggtcactc caggtgcatg gccacttccc gaggcgcctc gggggccggg
     2341 ccttgcgcgg ccttggtgcg cttgacgcgc aggaacagga gcagcgggat caccacgacg
     2401 aacgcgatgg tctgcaggag gaaggtcttg tcgaaaccga tcaccgtgcc ctgcagcagg
     2461 gtgcggccgg cgatcgactg ctgggtgagc gcgctggcgg cgtcggggcc catgccgtgg
     2521 ctcatgatcg tgctgcgggt catcatcgcc tgggcggcga cctcggggcg caggatggat
     2581 acgttctcgg ccaggccggc ggcggcgcgg tggctgtagt tggtgaacag gttggcgaag
     2641 atcgacaggc cgatcgagcc gccgacctgg cgcacgaagc tgttgacgcc ggcggccccg
     2701 gcgagctcgt ggcgcgcgac gttggccagc gcggcggtgg tcagcggcac gaacaggaac
     2761 gcgaagccga tcccggtgac cgccagcggc aggcggaggt ccgcgctcga ggtcgccagg
     2821 ttgatgtcgg cctgcatcca gcagcccagc gcggtcatga ccatgccgaa cgcgcacagc
     2881 cacgccggcc ggatctggtt gtacagccgg ccgacgatcg gcgacgcgac catcatcgcg
     2941 atcgtgcgcg gtagcagcac gacgccggac tcggtcgccg tgaacttcat catctcctgg
     3001 gtgaacaccg gcaggaggaa catcgagccc atcagcatcg cgaacaccag gcagccgatc
     3061 accgtgcccg aggcgaacgt cggatcgcgg aacatccgca ggtcgacgac cggcgcggtc
     3121 gcggtcagct cgcggatcac gaacgcgcac aggccgaccg cggcgatgta cgtgccgacc
     3181 aggatcgcgg tcgagtcgaa ccagtcgttc tgcgggccct cctcgaggac gtactgcagc
     3241 gcgccgatcg cgatcgccat cagcgcgatg ccggcccagt cgaggtgctt gcgcgccacg
     3301 gcggcgcgcg cacggttggc ggcgagcacg tcggcgggct cgatcacgtt gcgcatcgtg
     3361 aggtagatgc cgagcacgcc gaccgggagg ttgacgaaga agatccacgg ccacgagtag
     3421 ttgtcggtga tccagccgcc gagcaccggg ccgaccgccg ggccgaccat gatcaccatc
     3481 gagaacaccg ccatcgccat gccctgctcg gcgggcggga aggtctggcg caggatcgcc
     3541 tgctgggtcg gctgcagcgc gccgccgccg aacccctgga cgatgcgcca cagcaccagc
     3601 atctcgagcg agcgcgccgt gccgcacatc accgagccga tggtgaacac gaccagcgac
     3661 acgaggtaaa cccgcttctg gccgaagaac gagccgagga agccggtcag cggcatgacc
     3721 agcaccatgg cgatcatgta gccggtggtg atccaggtga tctcctggag cgatgcaccg
     3781 agctcgccac ggatctgcgg gatcgcgacg ttgacgatcg aggtgtcgat cgtcgacatc
     3841 accgagccga gcgcgatcga gatcgcgacc gtccacttgc cgacctggcg cggcgcgtgc
     3901 gacgggatga ggccgccggc gccggaagcg ggaggcgcgg cggtcatcgc agagccctcc
     3961 gcgcgccgcc gagcacgaag tcgacgaccg cgtcggcgtc acggaccagc tcggcgccgg
     4021 cctggatccg ctggaccacg atcgccttga tcgtcccggt gatcgcgagc gccaggaggt
     4081 cggtggcggc cgggatcgcg ccctcgcgga ccccggcggc gaggatgtcg cgcacgccgg
     4141 cggcgaggtc ctgcggcgcg gtcgaggccg acggcttcag gtgctcgttc tcgatcgcga
     4201 gcttgaggaa gctgcggtgc gactcgaacg cctcgaacag gtcgcgcacg aaccggcgca
     4261 gccggggctc gaacgggagg tcgaggccgg cgtcgatcgc ggcgagcatc cggggccgga
     4321 acgtggcgcg ccgggtctcg aacagcgcgc ggatcagcgc gtcgcggtcg gtgaagtagt
     4381 tgtacagggt gccgaccgcg accccggcgc gcctggcgat ctggaccagc gcggcgtcgt
     4441 gcagcccgtg gcgcgcgatc agctcctcgg cggcgtcgag gatcgcgagc cgcacggtct
     4501 cgcgcatcgt gcgccgcatc tcgctgcgca gctcggcgcg cgaggcgacc cgcgcggc
//
LOCUS       DASVOR010000668         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_503238,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000668 DASVOR010000000
VERSION     DASVOR010000668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503238"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF516_13210"
     CDS             complement(<1..401)
                     /locus_tag="VF516_13210"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEX8108684.1"
                     /translation="MIGQPYFGEIGNTRTSTVHVIDSRGALVAVEVHTRINAAADPEL
                     AEQLRDDSLNVVQLGGGEMRIAVPVVYHDPAARLMVLVLGDAHRHREIEERIRLLEQL
                     RDDVADIPGYAKDFVVVFGAEGLRALLADRAR"
     gene            complement(434..1105)
                     /locus_tag="VF516_13215"
     CDS             complement(434..1105)
                     /locus_tag="VF516_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108685.1"
                     /translation="MLDSVPPRVREYADQAVSYVRRALGIQLEYDSNTLPVLDHYLRT
                     VPEDQPAALQLVVATSGAYFGEVVRQRLGGRWELADQEAEWRIVLPTGLNFSPVGFVA
                     AAIARADLADLDSEIAAPPRMLPYVQRALARMGEVTIDDYYSLCGRLDTLEHVHEVLV
                     AVAAQMLGDEDLDDVDAAGDPDGAPGDDDDDDDEDDEDIADDDPLGDGAPEPVADDES
                     SGPVN"
     gene            complement(1139..>1692)
                     /locus_tag="VF516_13220"
     CDS             complement(1139..>1692)
                     /locus_tag="VF516_13220"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEX8108686.1"
                     /translation="SSAPLEVVNMDAGSDLDTASGPISVRNVQGEVLTHSVSGLTRLV
                     QVFGSVDAQTLASDLNLDTINGDRLFASVHHGRIEGRRVRARDVELTATEGQILLEAE
                     AALRGRLLVSTYKGDVDVQLRRHGAVMVRAQGTKVDLGAPMQPRPNGWVESSFGQVTG
                     DAAIVEIRSPQGNVRFAIIESRP"
BASE COUNT          232 a          599 c          617 g          244 t
ORIGIN      
        1 cgcgcccggt ccgcgagcag ggcgcgcagc ccttcggcgc cgaacacgac gacgaagtcc
       61 ttcgcgtagc ccgggatgtc ggcgacgtcg tcgcgcagct gctcgagcag gcggatccgc
      121 tcctcgatct cgcggtggcg atgcgcgtcg cccagcacca gcaccatcag gcgggcggcc
      181 gggtcgtggt agaccaccgg cacggcgatc cgcatctcgc cgccgccgag ctgcacgacg
      241 ttcaggctgt cgtcgcgcag ctgctcggcg agctccggat cggcggcggc gttgatcctg
      301 gtgtggacct ccaccgcgac cagcgcgccg cgcgagtcga tgacgtggac cgtggacgtc
      361 cgggtgttac cgatctcgcc gaaatacggc tgaccgatca cgtgcggctc cccggcccgc
      421 gtcgcgccat gggctagttg accggcccgc tcgattcatc gtcggcgacc ggctcgggcg
      481 cgccgtcgcc gagcggatca tcgtcggcga tgtcctcgtc gtcctcgtcg tcgtcgtcgt
      541 cgtcgtcgcc gggcgcgccg tccggatcac cggcggcgtc gacgtcgtcg agatcctcgt
      601 cgccgagcat ctgcgccgcc accgcgacca gcacctcgtg gacgtgctcc agggtgtcga
      661 gccgcccgca cagcgagtaa tagtcgtcga tcgtgacctc gcccatccgc gccagtgcgc
      721 gctggacgta cggcagcatg cgcggcggcg cggcgatctc ggagtcgaga tctgcgaggt
      781 cggcgcgcgc gatcgccgcg gcgacgaacc cgacgggcga gaagttgagg ccggtcggca
      841 gcacgatgcg ccactcggcc tcctggtccg cgagctccca gcgcccaccg agccgctggc
      901 ggacgacctc gccgaagtac gcgcccgagg tcgcgaccac gagctggagc gcggcgggct
      961 ggtcctcggg gacggtgcgc aggtagtggt cgagcaccgg cagcgtattg ctgtcgtact
     1021 ccagctggat cccgagggcc cgccggacgt acgagaccgc ctggtcggcg tactcgcgaa
     1081 cgcgcggggg gaccgaatcg agcatgtcgg ccagcgtacc gcggttcgcg gaaccgggct
     1141 acggccggct ctcgatgatc gcgaaccgga cgttgccctg gggcgagcgg atctcgacga
     1201 tggcggcgtc gccggtgacc tggccgaacg acgattcgac ccagccgttc ggccggggct
     1261 gcatcggtgc gccgaggtcg accttggtgc cctgcgcgcg gaccatgacc gcgccgtggc
     1321 ggcggagctg gacgtcgacg tcgcccttgt acgtggacac cagcaggcgg ccgcgcagcg
     1381 cggcctcggc ctcgagcagg atctggccct cggtcgcggt cagctcgacg tcgcgagcgc
     1441 ggacccggcg gccctcgatc cgaccgtggt ggaccgaggc gaacagccga tcaccgttga
     1501 tcgtgtcgag gttgagatcc gacgccaggg tctgggcgtc gaccgagccg aacacctgga
     1561 ccagccgggt cagccccgac acgctgtggg tcagcacctc gccctggacg ttgcgcaccg
     1621 agatcgggcc cgaggcggtg tcgaggtcgc tgccggcgtc catgttgacg acctcgagcg
     1681 gagcgctcga gg
//
LOCUS       DASVOR010000669         2991 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_35601,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000669 DASVOR010000000
VERSION     DASVOR010000669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2991)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2991)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2991
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35601"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..960)
                     /locus_tag="VF516_13225"
     CDS             complement(<1..960)
                     /locus_tag="VF516_13225"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108687.1"
                     /translation="MPNPQSGTILGAYRVEGTIGSGSMGEVFRGIDTGLNRRVAIKLL
                     SEKHRDSPELRQRFVREGRAVAAISHPNVVQVFATGSYDDRPYIAMELLDGADLGSIV
                     ERSGRLDSLAAAHAVLDAAQGLAAAARAGLIHRDVKPSNLVRLSDGKVKVTDFGLAKP
                     VDPGSEPALTAMGVVVGTPDYIAPEQARGEAIDERVDIYALGGTLYFLLVGMPPFRTG
                     KPAEDKYLKVVARHLRNPPPDALVANPGCDRELAELTRAMMAKKPAERPSYDELIAQL
                     SRIVSRLDPAAVPLLIAARDRLQPIALPPVERRSDESPAADGVG"
     gene            complement(1351..1662)
                     /locus_tag="VF516_13230"
     CDS             complement(1351..1662)
                     /locus_tag="VF516_13230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108688.1"
                     /translation="MNKHRSTQSRVSNDTQASNFEAGTALENLRAEIIDIEALARAAE
                     AAADGLPAPTTDHQRLAFGRIQSLATKTSEQASASLRFANAQVAALTAHLDAHRKATA
                     R"
     gene            2556..2912
                     /locus_tag="VF516_13235"
     CDS             2556..2912
                     /locus_tag="VF516_13235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108689.1"
                     /translation="MRDVRRTSPRIDTYALCWELVDGREVSGLAVDLGALGLCVERPY
                     IGGRTPSAVPLQLELPGTDEVMWATADPRFDTIIARGRQLIRRTGYRIALAARRDLCM
                     LQEIVIETARLRAAAR"
BASE COUNT          447 a         1023 c         1034 g          487 t
ORIGIN      
        1 gccgactccg tcggccgcgg ggctctcgtc ggaccgccgc tcgaccggcg gcagcgcgat
       61 cggctgcaag cggtcgcgcg ccgcgatcag cagtggcacc gccgccggat cgagccggct
      121 gacgatccgc gacagctgcg cgatcagctc gtcgtagctc ggccgctcgg ccggcttctt
      181 cgccatcatc gctcgcgtca gctcggcgag ctcgcggtcg cagcccgggt tggcgaccag
      241 cgcgtcgggc ggtgggttgc gcaggtggcg tgccaccacc ttcaggtact tgtcctcggc
      301 gggcttgccg gtgcgaaacg gcggcatccc gaccagcagg aagtacagcg tgccgccgag
      361 cgcgtagatg tcgacccgct cgtcgatcgc ctcgccgcgc gcctgctccg gtgcgatgta
      421 gtccggcgtg ccgaccacga cccccatcgc ggtcagcgcc ggctcgctcc ccggatcgac
      481 cggcttggcc agtccgaagt cggtgacctt gaccttgccg tccgacagcc ggacgaggtt
      541 cgacggcttg acgtcgcggt ggatcagccc ggcgcgcgcg gccgccgcca acccctgcgc
      601 agcgtcgagc accgcgtgcg ccgccgccag cgagtcgagc cggccgctgc gctcgacgat
      661 cgaccccagg tccgccccgt cgagcagctc catcgcgatg tacggccggt cgtcgtagct
      721 gccggtcgcg aacacctgca ccacgttggg atggctgatc gccgccaccg cccggccctc
      781 gcgcacgaac cgctggcgca gctcggggct gtcgcggtgc ttctccgata gcagcttgat
      841 cgcgacccgg cggttcagcc cggtgtcgat gccgcggaac acctcgccca tcgagcccga
      901 gccgatcgtc ccctcgacgc gataggcgcc caggatcgtt ccggactgcg ggttcggcac
      961 ggcaccattc tagcgccaaa cgcatagcgg ggcgtcgcgc cgcgcccgcc aatcctcgcc
     1021 ggaggacctc gccgtgcccg ccgcggcgca ggcggcagcc gcgtggcgcc atggtcgcgg
     1081 caatggtggg ggagtggcag ccacgctggc cgccacatcg aggcgacgca gcccggaagg
     1141 acctcgggag agcgcgaggg tcgcctgtga ccagccggag caagcggacc aggttgacct
     1201 tgaccgcctg ctcacagtcg ggggccccgc aggaggctcc gttcgggatt tccatccggg
     1261 atctgggagg aagagaggct gtttcatctg cccggctcgc cccatcgact ccagagcgct
     1321 gccggcgggg caagccgcgg atcgcctacg tcagcgagcg gtcgccttgc ggtgcgcatc
     1381 caggtgcgcg gtgagcgcgg cgacctgcgc gttggcgaag cggagcgagg cgctcgcttg
     1441 ctctgaggtc ttggtcgcca gcgactggat ccggccgaac gccaggcgct ggtggtcggt
     1501 cgtcggcgcc ggcagcccat cggcggctgc ctccgcggcg cgggcgagcg cctcgatgtc
     1561 gatgatctcg gcgcggaggt tctccagcgc ggtgccggct tcgaagttgc tggcctgcgt
     1621 gtcgttcgat accctggact gggtgctgcg atgcttgttc atcgtcggct ccttggatcg
     1681 tcggtgttcg agcaccggcg gtccactatg tcagtcgtgc cttgcgccca cggcggcgct
     1741 gccttgttcc gagggaacgt ttctacatgc gccggacagc gcaggaaatc aatcagattg
     1801 tgccagtcat tgatccatct attgaaaagt catgtccgtg ccctgggaca atgaccgtct
     1861 tctctgaatc caaaacaaac caaaaaactc tgcctggagc ctgagctcga gcgtcaggac
     1921 tgtgggcaac tgaagaaggg ctgtaagacg cgcccatgtc gtcctggcgc cccacggcgc
     1981 gtgtcatcag accttccagt tgtccacagg accgtccaca tgctctgcag agcttttgat
     2041 cgagggcgat cggcaccgaa tgctggcgac gcgctgcctc catcgccctc gagcgcgatc
     2101 cgtcgagcgg gtcggcgata catcgcgcag tctgtccgct ctgcagcacc gccgaacgcg
     2161 gcgcgcatga ccgcatggat ggatcagagt gcatcagttc gatcgctggt tgcgaagaac
     2221 tggagacatc tgtctctgat caaatgttgt tgtcgcgaaa tgattgcatt ccaggtgaat
     2281 gccgcttggt gggcaaacct gatgatgcga atggcaatta gtagtgctca ttcaattgac
     2341 attgtgcgca ggcatgatag tccaggggca tggcgcaggc gaagtcgaag cgtggttgca
     2401 agcggcagcc tcggcaacag gagctgttcc ggcgcggcgg caagcggcgc ggtgccgggc
     2461 gaaagcccaa ggggtcgcgc gccggggagc gccaccaggc gcggccggac ttcaagcgtt
     2521 acgatcgcgc gccacggcct gggacaattt ctgccatgcg agatgtccgc cggaccagcc
     2581 cccggatcga cacgtacgcg ctgtgctggg agctcgtcga tggccgcgag gtcagcggtc
     2641 tcgccgtcga cctcggcgcg ctcggcctgt gcgtcgagcg accctacatc ggcggtcgca
     2701 cgccgagcgc ggtcccgctc cagctcgagc taccgggcac cgacgaggtc atgtgggcca
     2761 ccgccgaccc gcgattcgat acgatcatcg cgcgcggccg tcagctgatc cgccggaccg
     2821 gctaccggat cgcgctcgcg gccaggcgcg acctgtgcat gctgcaggag atcgtgatcg
     2881 agaccgcgcg gcttcgcgcc gccgcccggt gagcggccga tccggcgcac gagtgacccc
     2941 gccgcgccgg cgccgggggc tggccgtgtc gcccggttga cggcgcgggg c
//
LOCUS       DASVOR010000670         1785 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_561996,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000670 DASVOR010000000
VERSION     DASVOR010000670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1785
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561996"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            315..1379
                     /locus_tag="VF516_13240"
     CDS             315..1379
                     /locus_tag="VF516_13240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108690.1"
                     /translation="MGLSLGAIFVLAGTLAGLAGCVDTAIRTAPFRSRPDAAEPGSLQ
                     GAFDGRVVDATTHAPIAGALVYAAWTFERGTGLAEPAGAKEFVGSTDAAGNYKVPRLD
                     RSPHGARVTEFTLLVYKRGFIAYRSDRRFSDLGPRMDFAQTDNQVLLERWRNELSHAR
                     HLRFVGAGTAVAALTQWEIADASAELDGRRPRSGDDLRPGRGEGQYIVAAQLLTETDI
                     KARTKYDGGFETGPLSDEPDTATYSSQHFKALGRPETWDVALRMWRLDPGKAQERYDE
                     LLTQLPGIVEKDDIASRSFVASENEIRGVGFLDAPRGVVVLMTCGSNQCISSEDAMSL
                     AQTVYGRVKSLLPVTLGGGK"
     gene            1376..>1785
                     /locus_tag="VF516_13245"
     CDS             1376..>1785
                     /locus_tag="VF516_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108691.1"
                     /translation="MRPLRRPRRLAMVLALALIPASAAAWEPQTTHAGLAEQAALASR
                     LHKRLVSLGFAGGLFEPLTIPPADAPALSAALKLLSPTHGSVPDARGRQAALAWLTAG
                     AALADFPASQAANHFFDPATGAGWIPPERGLGGRL"
BASE COUNT          235 a          679 c          618 g          253 t
ORIGIN      
        1 ctaccgcagt tcaagcagaa cgacgatccc gctgggcttt cgtggccgca catcgaacaa
       61 cgcatcgttt ccccgacatt gaacccgatc caagtgtctc cacaaccgcc cgccacggga
      121 atgggctccg gccggtttgc aacgccatga cggtcttgct aagacgggcc cgctccgtgg
      181 ccgccaactt cgcacgccgt ctcgcgttgt caatcctccg acgtcttggc cccgggcctg
      241 accccggttc tggcccggat gtcgaccccg ttgtcgaccc cgttggctgg cgcgtccgat
      301 cgacggcgct ctcggtgggg ctctcactcg gggcgatctt cgtgctcgcg gggacgctcg
      361 cggggctcgc cggctgcgtg gacaccgcga tccgcaccgc gccgttccgc agccgccccg
      421 acgctgccga gcccggctcg ctgcagggcg cgttcgacgg tcgcgtcgtc gatgcgacca
      481 cgcacgcgcc gatcgccggc gcgctggtct acgccgcgtg gacgttcgag cgcggcaccg
      541 gcctggccga gcccgccggc gccaaggagt tcgtcggctc gaccgacgcc gccggcaact
      601 acaaggtgcc gcggctcgat cgctcgcccc acggcgcgcg ggtcaccgag ttcacgctgc
      661 tggtctacaa gcgcggcttc atcgcgtacc gcagcgatcg ccggttcagc gatctcggcc
      721 cgcggatgga cttcgcgcag accgacaacc aggtgctgct cgagcgctgg cgcaacgagc
      781 tgtcgcacgc gcgccacctg cggttcgtcg gcgcgggcac cgcggtcgcc gcgctgaccc
      841 agtgggagat cgccgacgcg agcgccgagc tcgacggccg gcgccccagg agcggcgacg
      901 acctgcgccc cggccgcggc gagggccagt acatcgtcgc ggcccagctg ctcaccgaga
      961 ccgacatcaa ggcgcgcacc aagtacgacg gcgggttcga gaccgggcca ctgtccgacg
     1021 agcccgacac cgcgacctac tcctcgcagc acttcaaggc gctcggccgg ccggagacct
     1081 gggacgtcgc gctgcgcatg tggcggctcg atccgggcaa ggcccaggag cgctacgacg
     1141 agctcttgac ccagctgccg gggatcgtcg agaaggacga catcgcgagc cgctcgttcg
     1201 tcgcgtcgga gaacgagatc cgcggcgtcg gcttcctcga tgcgccgcgc ggcgtcgtcg
     1261 tgctgatgac ctgcggctcg aaccagtgca tctcgtcgga ggacgccatg tcgctggccc
     1321 agacggtcta cggccgggtc aagtcgctgc tgccggtcac gctcggaggt ggcaagtgag
     1381 gcccctgcgc cggccgcgcc ggcttgccat ggtgctcgcg ctcgcgctga tcccggcgag
     1441 cgcggcggcg tgggagccgc agaccaccca cgccggcctc gccgagcagg cggcgctggc
     1501 gtcgcggctg cacaagcggc tggtgtcgct gggcttcgcc ggtgggctgt tcgagccgct
     1561 gacgatcccg ccggcggacg ccccggcgct gagcgccgcg ctcaagctgc tatcgccgac
     1621 ccacggctcg gtgcccgatg cgcgcggccg gcaagccgcg ctcgcctggc tcaccgccgg
     1681 cgccgcgctc gccgacttcc cggcgagcca ggccgccaac cacttcttcg atccggccac
     1741 cggcgccggg tggatcccgc ccgagcgcgg gctgggcggc cggct
//
LOCUS       DASVOR010000671         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_123223,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000671 DASVOR010000000
VERSION     DASVOR010000671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123223"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /locus_tag="VF516_13250"
     CDS             <1..1233
                     /locus_tag="VF516_13250"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8108692.1"
                     /translation="RTADGTYEAFLYAGLSSVFGTPEAIATVEQSSAHYFQRPDAGYL
                     HVDTDARRLVGWHAGAVGTKRAGEWQGTALANVESPGFELNDLGVLQSADDINLSADL
                     RRTVIRPGERVFQWDAAAGASTSWNFGGLRKPVDLRASGDITSHAFNSAAIALDVTTP
                     GGSDDLTRGGPVMQTGWAEAVTLTATTPRGRAQQLSGSLVGQLSSTLQQGLVASASLA
                     SRVTPALRLDLTPSFTWVETQRQYVTTVTGAGGGERTFGARYLFGQLHRKEAALVIRA
                     TWSLSPELVITLYAQPFASVGRYDRLGELAAAGSGDVRWYEATSRTGPMRAIADGNGG
                     FSTGFSIDEPDFTVLSLRSTAVLRWELSPGSTLFIVWQQSRQGSTTRAQPLHVAAPDV
                     VTQPGVHSLAIKLSYWFG"
     gene            1338..>1738
                     /locus_tag="VF516_13255"
     CDS             1338..>1738
                     /locus_tag="VF516_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108693.1"
                     /translation="MIAISTFAMGCATTSTYSTEYTADEYSPREGVVESVRQIVHRVE
                     GNPAGGAVAGALIGAVLFGGHGPRTLFGAAAGAATGAALSQGSYETRAYEVVVRFDNG
                     ARGVFTYRDYSPFAPGDRIALGPDGPHRVIY"
BASE COUNT          233 a          641 c          613 g          251 t
ORIGIN      
        1 cgcaccgcgg acggcaccta cgaggccttc ctgtacgccg gcctgagctc ggtgttcggc
       61 accccggagg cgatcgcgac cgtcgagcag agctcggcgc actacttcca gcgccccgac
      121 gcgggctacc tccacgtcga caccgacgcg cgccggctgg tcggctggca cgccggggcg
      181 gtcggcacca agcgcgccgg cgagtggcag ggcaccgcgc tggccaacgt cgagtcgccg
      241 gggttcgagc tcaacgacct cggcgtgctg cagtccgccg acgacatcaa cctgtcggcc
      301 gacctgcgcc gcaccgtcat caggccgggc gagcgcgtgt tccagtggga cgccgccgcg
      361 ggcgcgagca cgtcgtggaa tttcggaggg ctgcgcaagc cggtcgatct gcgggccagc
      421 ggcgacatca cgtcgcacgc gttcaactcg gcggcgatcg cgctcgatgt caccacgccg
      481 ggcggctccg atgacctgac ccgcggcggc ccggtgatgc agaccggctg ggccgaggcg
      541 gtgacgctga ccgcgaccac cccccgcggc cgcgcccagc agctgtcggg gtcgctggtc
      601 ggccagctca gctcgaccct gcagcagggc ctcgtcgcgt cggcgagcct ggcctcgcgc
      661 gtgacgcccg cgctgcggct cgatctgacc ccatcgttca cctgggtcga gacccagcgc
      721 cagtatgtca ccaccgtgac cggcgccggc ggcggcgagc gcacgtttgg cgcgcgctac
      781 ctgttcggcc agctccaccg caaggaggcc gcgctggtga tccgcgcgac gtggtcgctg
      841 tcgcccgagc tggtcatcac gctgtacgcc cagccgttcg cgtcggtcgg ccgctatgac
      901 cggctcggcg agctggccgc cgccggctcc ggcgacgtgc ggtggtacga ggcgacctcg
      961 cgcaccgggc cgatgcgcgc gatcgcggac ggcaacggcg gattctcgac cgggttctcg
     1021 atcgatgaac cagacttcac cgtgctgtcg ctgcgctcga ccgcggtgtt gcgctgggag
     1081 ctgtcgcccg gcagtacgct gttcatcgtg tggcagcaat cccggcaggg cagcaccacg
     1141 cgcgcgcaac cgctgcacgt cgccgcgccg gacgtcgtga cgcagcccgg cgtgcatagc
     1201 cttgcgatca agctgtctta ctggttcggt tgacggaatg gcccgtgcct tctccgccgg
     1261 cacatcgccg gcacaccgcc ggcacaccgg atgcatgact ccggtgcatg agagtcgcca
     1321 gaacctgtgc cgcagcgatg atcgcgatat cgacgtttgc gatgggctgc gcgaccacca
     1381 gcacgtattc caccgagtac accgccgacg agtacagccc gcgcgagggc gtcgtggagt
     1441 cggtccgcca gatcgtgcac cgcgtcgagg gcaaccccgc cggcggcgcg gtcgccggcg
     1501 cgctgatcgg cgcggtgctg tttggcggcc acggcccgcg gacgctgttc ggcgcggccg
     1561 ccggcgctgc caccggcgcg gcgctgagcc agggtagcta cgagacccgc gcctacgagg
     1621 tcgtggtccg gttcgacaac ggcgcgcgcg gcgtgttcac gtaccgcgac tactcgccgt
     1681 tcgcgcccgg cgaccggatc gcgctcggcc ccgacggtcc gcaccgcgtg atttactg
//
LOCUS       DASVOR010000672         1809 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_269300,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000672 DASVOR010000000
VERSION     DASVOR010000672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1809
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269300"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            227..829
                     /locus_tag="VF516_13260"
     CDS             227..829
                     /locus_tag="VF516_13260"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX8108694.1"
                     /translation="MHRQLDLLAQQRIGFDASFAELRRVELADGAWVDHVPGWVTGHD
                     LLFEQLERSLVWCTETRRMYDRKVATPRLLATIPATEVAGGVVEDMRRALSARYGEDF
                     VRTTAALYRDGNDSVAWHGDTTARDMERATVATVSLGQPRKFLLKPTAGGASIAYQLG
                     RGDLLVMGGTCQRTWRHAIPKAAHAPGPRIAVMFRPDWGY"
     gene            874..>1809
                     /locus_tag="VF516_13265"
     CDS             874..>1809
                     /locus_tag="VF516_13265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108695.1"
                     /translation="MRAIDWVPGAVIAERYRLVRRVGEGGMGVIWAADDLVTGRRTAL
                     KRIKDAVDDPAARRRFLHEARAASVVRHPNVVEMFDVLELETGPPVLAMELLDGESLR
                     DVLVRDRTLSVPALADVLVPVIGAVGAAHALGVVHRDLKPENIFLARGADGARVVKVL
                     DFGIAKLTALDGEAMRSAGLTTTAVIGTPAYMAPEQVFGERDIDHRADIWALGVVVYE
                     CLAGVRPTEGDNVGQVLKHVVARPFVPLAQQVDGVPDGVSRLVERMLQRERSHRIADL
                     HDALAVLAPFASAPGVPFGAPAAHFARADAAVAETV"
BASE COUNT          224 a          677 c          660 g          248 t
ORIGIN      
        1 cgtgatcttc ggccactggg ccgcgctcgg cctcgacatc ggcccgcacc acgtcggcct
       61 cgacaccggc tgcgtgtggg gccgctcgct gaccgcgatc cggctcgacg accgcatggt
      121 gttccaggtc aaggccgtcg aatcggccag ctgagccgcc gccggagccg cgtcctggcc
      181 agccggccag ctcgccgccg ccgcgccgcg gtgctatccg cccgccatgc accggcagct
      241 cgacctgctc gcgcagcagc gcatcggctt cgatgcgagc tttgccgagc tgcgccgcgt
      301 cgagctcgcc gacggcgcct gggtcgacca cgtcccgggc tgggtcaccg gccacgacct
      361 gctgttcgag cagctcgagc gctcgctggt ctggtgcacc gagacccggc ggatgtacga
      421 ccgcaaggtg gccacgccgc ggctgctcgc caccatcccg gcgaccgagg tcgcaggcgg
      481 cgtggtggag gacatgcgcc gcgcgctgtc ggcgcgctac ggcgaggact tcgtgcgcac
      541 caccgccgcc ctgtaccgcg acggcaatga cagcgtcgcc tggcacggcg acaccaccgc
      601 gcgcgacatg gagcgcgcca ccgtcgccac ggtctcgctc ggccagcctc gcaagttcct
      661 gctcaagccg accgccggcg gcgcctcgat cgcctaccag ctcggccgcg gcgatctgct
      721 cgtcatgggc ggcacctgcc agcgcacctg gcgccacgcg atccccaagg ccgcccacgc
      781 cccgggcccg cggatcgccg tcatgttccg gcccgactgg ggctactgac gccgcggcgt
      841 gaaccgcgaa ggccgatcgg gcaaagtagc tggatgcgcg cgatcgactg ggtgcctggc
      901 gccgtgatcg ccgagcgcta tcgcctcgtc cgccgcgtcg gcgagggcgg gatgggcgtg
      961 atctgggccg ccgacgacct ggtcaccggc cggcgcaccg cgctcaagcg gatcaaggac
     1021 gccgtcgacg atccggcggc gcgccgccgg ttcctgcacg aggcgcgcgc ggcctcggtc
     1081 gtgcgccacc cgaacgtggt cgagatgttc gacgtgctcg agctcgagac cgggccgccg
     1141 gtgctcgcga tggagctgct ggacggcgag tcgctgcgcg acgtgctggt ccgcgaccgg
     1201 acgctgtccg tcccggcgct cgccgacgtc ctggtgccgg tgatcggcgc ggtcggtgcc
     1261 gcgcacgcgc tcggcgtcgt gcaccgcgat ctcaagccgg agaacatctt cctggcccgc
     1321 ggcgccgacg gggcgcgcgt cgtcaaggtg ctcgacttcg ggatcgccaa gctgaccgcg
     1381 ctcgacggcg aggcgatgcg ctcggccggg ctcaccacga cggccgtgat cgggacgccc
     1441 gcgtacatgg cgcccgagca ggtgttcggg gagcgcgaca tcgatcatcg cgccgatatc
     1501 tgggcgctcg gcgtggtggt ctacgagtgc ctggcggggg tccgaccgac cgagggcgac
     1561 aacgtgggcc aggtgctgaa gcacgtggtg gcgaggccgt tcgtcccgct cgcgcaacag
     1621 gtcgacggtg tcccggacgg cgtgtcgcgg ctcgtcgagc gcatgctgca gcgcgagcgc
     1681 tcgcaccgga tcgccgacct gcacgacgcg ctcgcggtgc tcgcgccgtt cgcgagcgcg
     1741 ccgggtgtgc cgttcggcgc gccggcggcg cacttcgcgc gagccgacgc cgcggtcgcc
     1801 gagaccgtc
//
LOCUS       DASVOR010000673         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211069,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000673 DASVOR010000000
VERSION     DASVOR010000673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211069"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1660
                     /locus_tag="VF516_13270"
     CDS             <1..1660
                     /locus_tag="VF516_13270"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX8108696.1"
                     /translation="IWFAGLGIAALTALPRLAAAGGLLLPGAGAVSTSRAGAGVASAD
                     DGEALVLNPAGIAKATGTTITLSAAIIDFAMEFQRRGSYDAVAGESYPYAGQSFAAVK
                     NDASPPLGIGAMQPVPVIAVLSDLGGRLGGLHLGVGVYAPNAYPFRDLCTELSGGCQK
                     YQFGDNPSIPPPPTRYDLMKQEAAVFLPSLAAAYRILPELDVGLRLSWGVANLKSTTA
                     LWGSPTANYEEDIKKDGVINADVTDSFVFGYGLGATYRPTPNLELALNYSSELDIYGK
                     GTATSQLGPSAGLPGLPTTIAPKPDNMATCAPGGTTDALKACVDLALPRSAQLAGRYK
                     FLDAAGRLRGDVELDLDWENWGKTCSDGDFNAGKCVSPGDYRVVIDAAVVLNGLAMIP
                     LNDAKLSHGFQDTYGVRAGGSYHLPVGAARGDGSHDELILRGGLGYETAAARTGWLRA
                     DIDGAARTTITAGAAYRLQRYEFSLGGGAILEGSPSNPNVGGGAAPCNPTMADMTCNG
                     STAHQGPDPINPIRTSDDQVLNPVNQGDYKSHYLLLMLGVSAWF"
     gene            1704..1976
                     /locus_tag="VF516_13275"
     CDS             1704..1976
                     /locus_tag="VF516_13275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108697.1"
                     /translation="MGNSLIKSSRGRRANAAGILPAACSAVVPGLGQLVNGDTDKALG
                     VFAVAAVSGLGFLHIIPIIGPVAGLVYGATWLYGVADGFLEGRKKR"
     gene            2062..2889
                     /locus_tag="VF516_13280"
     CDS             2062..2889
                     /locus_tag="VF516_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8108698.1"
                     /translation="MSDVFDQFQGTASYIASDELRHAVNVAVALARPLLVRGEPGTGK
                     TLLAENLASALRLPLIRWHVKSTTKAKDGLYVYDTVARLHDSRFGDAADVRDIARYIK
                     LGPLGEALAAPSRVVLLIDEIDKADLEFPNDLLHELDVMRFRIDETGREIAAAERPIV
                     VITSNNEKELPDAFLRRCVFHYIQFPSRELMTEIVRVHHPDITDRVLDNALEVFFGLR
                     ATPRLRKKPSTSELIDWICALKKAGVDLTRVSGGIPFLGTLLKTEQDVETVTKRAKA"
     gene            2904..>3595
                     /locus_tag="VF516_13285"
     CDS             2904..>3595
                     /locus_tag="VF516_13285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA containing CoxE family protein"
                     /protein_id="HEX8108699.1"
                     /translation="MLIDFLFELRSRKLPVSTHEWMALMEAMALGLHESSLDGFYQLC
                     RTLCVKDIALYDAYDEAFLAYFKDVHLDALRLTEQLAAWLANPHALDGLTEEQRKALQ
                     ALDIEKLRQLFEQRLKEQKGRHDGGNRWIGTGGTSPFGTGGKNPNAMRAGGGGGRSAM
                     QVADERRFREYRRDVVLDVRQIDVALRGLRRLGREGAQEELDLDETVDKTCRNAGEIE
                     VVFRPPRRNRVKV"
BASE COUNT          557 a         1288 c         1245 g          505 t
ORIGIN      
        1 gatctggttt gcggggctcg ggatcgccgc actcaccgcg ctgccccggc tcgccgcggc
       61 gggcggcctg ctgctgccgg gcgccggggc ggtatccacg tcgcgcgccg gtgccggggt
      121 ggcgtcggcc gacgacggcg aggcgctggt gctcaacccg gcggggatcg cgaaggcgac
      181 cggcacgacg atcacgctgt cggcggcgat catcgacttc gcgatggagt tccagcgccg
      241 cggcagctac gacgcggtgg ccggcgagag ctatccgtat gcgggacagt cattcgccgc
      301 ggtgaagaac gacgcgagcc cgccgctcgg gatcggggcg atgcagccgg tgccggtgat
      361 cgcggtgctg agcgatctgg gcggccggct cggcggcctg cacctcggcg tcggggtgta
      421 cgcgcccaac gcctatccgt tccgcgatct gtgcaccgag ctgtccggcg gctgccagaa
      481 gtaccagttc ggtgacaacc cgagcatccc gccgccgccg acccgctacg acctgatgaa
      541 gcaggaggcg gcggtgttcc tgccgtcgct ggcggcggcc taccgcatcc tgcccgagct
      601 cgacgtcggc ttgcggctgt cgtggggggt cgccaacctc aagtcgacca ccgcgctgtg
      661 gggctcgccg accgcgaact acgaggagga catcaagaag gacggcgtga tcaacgccga
      721 cgtcaccgac agcttcgtgt tcgggtacgg gctcggcgcg acctaccggc cgaccccgaa
      781 cctcgagctc gcgctgaact attcctcgga gctcgacatc tacggcaagg gcacggcgac
      841 ctcgcagctc ggcccgtccg ccggccttcc cgggctgccg accacgatcg ccccgaagcc
      901 cgacaacatg gcgacctgcg cgccgggcgg caccaccgac gcgctcaagg cgtgcgtcga
      961 tctcgcgctg ccgcgcagcg cgcagctcgc cgggcgctac aagttcctcg acgccgccgg
     1021 ccggctgcgc ggcgacgtcg agctcgacct cgactgggag aactggggca agacctgctc
     1081 ggacggcgac ttcaacgccg gcaagtgcgt cagcccgggg gactaccggg tcgtgatcga
     1141 cgccgcggtg gtcctcaacg gcctggcgat gatcccgctc aacgacgcca agctttcgca
     1201 cgggttccag gacacctacg gcgtccgcgc cggcggcagc taccacctcc cggtcggcgc
     1261 cgcgcgcggc gacggcagcc acgacgagct gatcctgcgc ggcggcctgg gctacgagac
     1321 cgcggcggcc aggaccggct ggctgcgggc cgacatcgac ggcgcggcgc gcaccacgat
     1381 caccgccggc gcggcgtacc gcctgcagcg ctacgagttc agcctgggcg gcggcgcgat
     1441 cctcgagggc tcgccgtcca accccaacgt cggcggcggg gcggcgccgt gcaacccgac
     1501 catggccgac atgacctgca acggcagcac ggcccaccag ggccccgatc cgatcaatcc
     1561 gatccggacc tccgacgacc aggtgctgaa cccggtcaac cagggcgact acaagtccca
     1621 ctacctgctg ctcatgctgg gcgtctcggc gtggttttga gccgggccct gtcaggtccg
     1681 acgacccgtg tcataaagga ggcatgggta actcgctgat caagtcgagc aggggcaggc
     1741 gtgccaacgc cgcggggatc ctcccggcgg cgtgcagtgc ggtggttccc ggtctggggc
     1801 agctggtcaa cggcgacacc gacaaggcgc tcggtgtgtt cgcggtcgcc gcggtgtcgg
     1861 ggctcggctt cctgcatatc atcccgatca tcggcccggt tgccggcctg gtgtacggcg
     1921 ccacgtggct gtacggcgtc gccgacgggt tcctcgaggg ccgcaagaag cgttgagcgg
     1981 aaacggagcg ttccgggggc cgtccggtgc cttttcagtc agcactgacc gtttctcgga
     2041 aaatacgcta aggtcggccc aatgtcggat gtcttcgatc agttccaggg tacggccagc
     2101 tacatcgcct ccgacgagct ccgccatgcg gtgaacgtcg cggtcgcgct cgcccggccg
     2161 ctcctggtgc gcggcgagcc gggcaccggc aagacgctgc tcgccgagaa cctcgccagc
     2221 gcgctccgcc tgccgctgat ccgctggcac gtcaagtcaa cgaccaaggc caaggacggg
     2281 ctgtacgtct acgacaccgt ggcccggctg cacgacagcc ggttcggcga cgcggccgac
     2341 gtccgcgaca tcgcgcgcta catcaagctc ggcccgctcg gcgaggcgct cgccgccccg
     2401 tcgcgcgtcg tgctgctgat cgacgagatc gacaaggccg acctcgagtt ccccaacgac
     2461 ctcctgcacg agctcgacgt gatgcggttc cggatcgacg agaccggccg cgagatcgcc
     2521 gccgccgagc ggccgatcgt cgtgatcacg tcgaacaacg agaaggagct gcccgacgcg
     2581 ttcctccggc gctgcgtgtt ccactacatc cagtttccca gccgcgagct gatgaccgag
     2641 atcgtccgcg tccaccaccc cgacatcacc gaccgcgtgc tcgacaacgc gctcgaggtg
     2701 ttcttcggcc tgcgcgcgac cccgcggctg cgcaagaagc cgtcgacctc cgagctgatc
     2761 gactggatct gcgcgctcaa gaaggccggc gtcgacctca cgcgcgtgtc cggcggcatc
     2821 ccgttcctcg gcaccctgct gaagacggag caggacgtcg agacggtcac caagcgggcg
     2881 aaggcctgag acgggagagc tcggtgctga tcgacttcct gttcgagctg cggtcccgca
     2941 agctgccggt gtcgacccac gagtggatgg cgctgatgga ggcgatggcg ctcggtctgc
     3001 acgagagctc gctcgacggc ttctaccagc tgtgccgcac gctgtgcgtc aaggacatcg
     3061 cgctgtacga cgcgtacgac gaggcgttcc tggcctactt caaggacgtc cacctcgacg
     3121 cgctccggct caccgagcag ctcgccgcgt ggctggccaa cccgcacgcg ctcgacggcc
     3181 tgaccgagga gcagcgcaag gcgctccagg cgctcgacat cgagaagctg cgccagctgt
     3241 tcgagcagcg gctcaaggag cagaagggcc gccacgacgg cggcaaccgc tggatcggca
     3301 ccggcggcac ctcgccgttc ggcaccggcg gcaagaaccc caacgcgatg cgcgcgggcg
     3361 gcggcggcgg ccgcagcgcg atgcaggtcg ccgacgagcg ccggttccgc gagtaccgcc
     3421 gcgacgtcgt gctcgacgtc cgccagatcg acgtcgcgct gcgcggcctg cgccggctcg
     3481 gccgcgaggg cgcgcaggag gagctcgacc tcgacgagac ggtcgacaag acctgccgga
     3541 acgccggcga gatcgaggtc gtgttccgcc cgccgcgccg caaccgggtc aaggt
//
LOCUS       DASVOR010000674         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_182092,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000674 DASVOR010000000
VERSION     DASVOR010000674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182092"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1477)
                     /locus_tag="VF516_13290"
     CDS             complement(119..1477)
                     /locus_tag="VF516_13290"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8108700.1"
                     /translation="MASSGIHVCLASCWAICALTDTGCDSLLGKEPNPGYCGSATCPD
                     AGGCRSNADCTSPGAGVCDVMATKQCVQCTATDHAACTGNAPVCSTDNTCRACAAHEE
                     CDSNACSFATGACAAETDVAYVSSTGADNSTCDKSAPCASITAALMTGRHYVKIHGTI
                     DGAVVVDKGRTVTLLADPSAMLTRGSGGDVLTVKDTGTSLTIYDLTISGAQTPSVGIA
                     LAGGVPALSLIRVTVSNNAGGGIASAGSLTIDHSTITGNGSGGPGISITGGSLSLAQS
                     LVSANHGGGVDVTGTTAKFAIVSNKFVGNGAGDPMGSLIGAVNIRANFAITNLLEFNT
                     FYKNLSTDGIGAAIQCDPATFTARANIMFGNGTATNLDPTSGTCMHMYSLAKPGTVPT
                     GTGNLGSDPLFVDAPGGNFHLQAGSPAIGAANALAPLTGLSARDFDDHPRTSPVNLGA
                     LQ"
     gene            complement(1489..2541)
                     /locus_tag="VF516_13295"
     CDS             complement(1489..2541)
                     /locus_tag="VF516_13295"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8108701.1"
                     /translation="MSRRSAIPALIAGTLAAWLASPRAAHAERDVLVVGGGAAERGQL
                     AAVRGAIENTLRKAGWSWPARPPSQKEADSLLKCEDSRSPWTCIPATISGLRASDVQR
                     VFVLSVESRAAPERMLVLTARVIVASSPPAFVVRQRFCEQCSTGQLVQASEELTRQVF
                     GELDARATATAVAVTSDPPDADVRLDGSPAGKTPARLFTGPGKHLVTVTKPDFVYEVR
                     TIDVEPGQTVAVGFTLHAAPSAAVPPPSDPTPGSSPATAPPESRSFRVIPAAAITAGT
                     GLVAFGGYALYLGHLGGPTDRYRYTRATPVGVTSIVLGLGAVGAGLYLWRGPDGSGLF
                     GAVDRHSSSVVWSGRF"
     gene            complement(2538..2921)
                     /locus_tag="VF516_13300"
     CDS             complement(2538..2921)
                     /locus_tag="VF516_13300"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8108702.1"
                     /translation="MQLAQAPTDPSRPAAPPRSSPGAPVAPSAVAPRSPPSPRPSSSG
                     AGGSPAAPSTAATPAVKNGELAIVVRPWALIWLNGKSLGQTPFREAIPAGRYRLRIAN
                     DDVGKDETTLVTVNPDQTTTVQRSW"
BASE COUNT          426 a          978 c         1120 g          446 t
ORIGIN      
        1 ggccgcgcgt cgggcgccgc cagcgcgggc gcggagagtg cacagccgat cacgatcgcc
       61 ccggcgatgc aggcggcccg gcgaggagca gcggtgaggc ggagcgtatg gcgcagggtc
      121 actggagcgc gccgaggttg accgggctcg tccggggatg gtcatcgaag tcgcgcgcgg
      181 acagtccggt gagcggtgcc agcgcgttcg ccgcgccgat cgcggggctt cccgcttgca
      241 gatggaagtt gccgccgggg gcgtccacga acaggggatc gctgcccaga ttgccggtgc
      301 cggtggggac cgtgcctggt ttcgcgaggg agtacatgtg catgcaggtc ccgctggtcg
      361 gatcgaggtt ggtcgcggtg ccgttgccga acatgatgtt ggcgcgcgcc gtgaacgtcg
      421 ccgggtcgca ctgaatcgcg gcgccgatcc cgtcggtgct caggttcttg tagaaggtgt
      481 tgaactccag gaggttggtg atggcgaagt tggcccggat gttcaccgcg ccgatcagcg
      541 agcccatcgg gtcgcctgcg ccattgccca cgaacttgtt gctcacgatg gcgaacttgg
      601 cggtggtgcc agtgacatcg acgccaccgc cgtggttcgc gctgaccaag ctctgtgcga
      661 ggctgagcga gccgccggtg atggagatcc ccggcccgcc gctgccattg ccggtgatgg
      721 tgctgtgatc gatcgtgagc gatcccgcag aggcaattcc gccgccggcg ttgttcgaga
      781 cggtgacccg gatcagtgac agcgccggga caccgccggc gagtgcgatg ccgaccgatg
      841 gcgtctgcgc accggagatc gtgaggtcgt agatcgtgag cgacgtgccg gtatccttga
      901 ccgtcaacac gtcgcccccg gatccgcgtg tgagcatcgc gctcgggtcg gccagcagcg
      961 tcaccgtgcg gcccttgtcg acgacgaccg cgccgtcgat ggtgccgtgg atcttgacgt
     1021 agtggcggcc ggtcatcagc gcagcggtga tgctcgcgca cggcgccgac ttgtcgcagg
     1081 tcgagttgtc ggcgccggtc gaggacacgt acgcgacgtc ggtctcggcg gcgcacgcgc
     1141 cggtcgcgaa cgagcatgcg ttggagtcgc actcctcgtg cgcagcgcag gcccggcagg
     1201 tgttgtcggt gctgcagacc ggggcgttcc cggtgcacgc ggcgtggtcg gtcgccgtgc
     1261 actgcacgca ctgcttcgtt gccatcacat cgcacacgcc cgcgccgggc gacgtgcaat
     1321 cggcgttcga gcggcagccg ccggcgtccg ggcacgttgc gctgccgcag tagcccgggt
     1381 ttggctcctt gccgagcagg ctgtcgcagc cggtgtcggt cagggcgcag atcgcccagc
     1441 acgacgcgag acacacatgg attcctgaac tggccatcac atgtctcctc aaaagcgtcc
     1501 gctccacacc acggacgagc tgtgtcgatc gacggcgccg aacagcccgg aaccatccgg
     1561 gccgcgccag aggtagagcc cggcgccgac cgcgccgagg ccgagcacga tcgacgtgac
     1621 gccgaccggc gtggcgcggg tgtagcgata gcggtcggtc ggcccgccga ggtgtccgag
     1681 gtagagcgcg taaccgccga acgccaccag cccggtgccc gcggtgatcg ctgccgcggg
     1741 gatcacgcga aacgaccggg actcgggggg cgcggttgcc ggcgaggagc ctggcgtcgg
     1801 gtcactcggc ggcggaaccg cggccgacgg tgcggcatgc aaggtgaaac cgaccgcgac
     1861 ggtctggccc ggctcgacat cgatcgtccg gacctcgtac acgaagtcgg gcttggtgac
     1921 cgtgaccagg tgtttgcccg ggccggtaaa cagccgcgcg ggagtcttgc cggcgggcga
     1981 gccatcgagg cggacgtccg cgtcgggtgg gtcggaggtc accgcgaccg cggtcgccgt
     2041 ggcgcgcgca tcgagctcgc cgaacacctg gcgggtgagc tcctcgctgg cctggaccag
     2101 ctgaccggtc gagcactgct cgcagaaccg ctggcggacg acgaacgccg gcgggctcga
     2161 ggcgacgatg acccgcgcgg tgagcacgag cattcgctcc ggggcggccc tggattcgac
     2221 ggacagtacg aacacgcgct ggacgtcgct cgcgcggagc ccgctgatcg tcgcggggat
     2281 gcaggtccac ggcgagcgcg aatcctcgca cttgagcagg ctatcggcct ccttctgcga
     2341 cggcggccgc gcgggccagc tccatcccgc cttgcgcagg gtgttctcga tcgcgccgcg
     2401 caccgcggcg agctggccgc gctccgcggc gccgccaccg accacgagca catcgcgctc
     2461 ggcgtgcgcc gctcggggag atgccagcca ggccgcgagc gtccccgcga tcagcgccgg
     2521 aatcgcggat cgccggctca ccacgacctc tggacggtgg tggtctggtc cgggttgacc
     2581 gtgaccagcg tggtctcgtc cttgcccaca tcgtcattgg caatgcgcaa tcgatacctc
     2641 ccggccggga tggcctcgcg gaacggtgtc tggccgagcg atttcccgtt gagccagatc
     2701 agcgcccatg gacggacaac gatcgcaagc tcgccgttct tgacggccgg ggttgcggcg
     2761 gtggagggcg ccgccggcga tccgccagcg ccgctggaag acgggcgagg cgatggcggc
     2821 gatctcggtg cgacggccga tggcgccacg ggcgcgccgg gtgacgagcg aggtggcgcg
     2881 gcggggcgtg aaggatcggt aggtgcctga gccagctgca cccgttctgc ggccggctgt
     2941 gcggcgtgcg ctggaccgac gggagccgcg
//
LOCUS       DASVOR010000675         2391 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211097,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000675 DASVOR010000000
VERSION     DASVOR010000675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211097"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VF516_13305"
     CDS             <1..262
                     /locus_tag="VF516_13305"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108703.1"
                     /translation="CAVTPDGRRVVSASGDGTLKVWDLASGRAAATLAGHADWVTACA
                     VTPDGRRVVSASRDKTLKVWDLASGRAESTLEGHGGSVTACA"
     gene            280..1374
                     /locus_tag="VF516_13310"
     CDS             280..1374
                     /locus_tag="VF516_13310"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8108704.1"
                     /translation="MVSASRGKTLKVWDLTSGRLEATLAGHAGSVTACAVTPDGRQMV
                     SASNDQTLKVWDLQSGACILTHRVNATCLAIATTATTIIAGDAAGAVWFLDWPSPDAR
                     QGSAQDDRRPDNQPTPSSDRELPAQRPPRKHTILFLAANPLGTDRVALDREARAIQVE
                     LERSGFRDRFELVTRWAAEPLDLLRELRKLKPTVVHFSGHGTAGVAGHASGTKRHRDV
                     DIEPGHNGGDPCSGLLFQDADGGPQLVSTEALEATFGAAGSSVQLTVLSACYSELNAQ
                     ALLQHVDCVVGMSGAIADDAARSFAIGFYGGLGERESIAAAYAQGCAAISLQGLSDRD
                     RPKLAVRTGIDAGKLVLAAYAPDRAAGRGG"
     gene            complement(1808..2113)
                     /locus_tag="VF516_13315"
     CDS             complement(1808..2113)
                     /locus_tag="VF516_13315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX8108705.1"
                     /translation="MGIFDRLRGGNVRIHILIKGRIGDDWKDIDEYVRVPAGTTLGKL
                     LDITDAARLPLREALAHSPHLTDTMMLNGERCPLHANEDRVLADGDEIYLLAPLAGG"
     gene            complement(2125..>2391)
                     /locus_tag="VF516_13320"
     CDS             complement(2125..>2391)
                     /locus_tag="VF516_13320"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8108706.1"
                     /translation="CGERIQNLRAAFNRREGLAPKDFQPHRRMLGEGDGQLEAGPLKG
                     IRVPLPVLRDDYYKSMHWNTTTGHLARSRAAELGMADLLDGYLE"
BASE COUNT          327 a          815 c          906 g          343 t
ORIGIN      
        1 gtgcgcggtg acgccggacg ggcggcgggt ggtctcggcg tcgggggacg gaacactgaa
       61 ggtgtgggac ctggcgagcg ggcgcgcggc ggcgaccctg gcaggccatg ccgactgggt
      121 gaccgcgtgc gcggtgacgc cggacgggcg gcgggtggtc tcggcgtcgc gggacaagac
      181 gctcaaggtg tgggacctgg cgagcgggcg cgcggagtcg acgctggaag gccacggcgg
      241 cagtgtgacc gcgtgcgctt gacgccggac gggcggcggg tggtctcggc gtcgcggggc
      301 aagacgctca aggtgtggga cctgacgagc gggcgcctgg aggcgacgct ggcaggccac
      361 gccggcagtg tgaccgcgtg cgcagtgacg ccggacgggc ggcagatggt ctcggcgtcg
      421 aatgaccaga cgctgaaggt gtgggacctg cagtccggcg cgtgcattct cacccaccgt
      481 gtcaacgcca cctgccttgc gatcgccacg accgcgacca ccatcatcgc cggcgacgcc
      541 gcgggcgcgg tctggttcct cgactggccg tcgccagatg cccgccaagg ttcagcccaa
      601 gacgatcgca ggcccgacaa ccagcccact ccgtcctcgg atcgcgagct ccctgcgcag
      661 aggccgccga ggaagcacac gatcctgttc ctggccgcca atccgctcgg caccgaccgg
      721 gtcgcactcg accgcgaggc gcgcgcgatc caggtcgagc tggaacgcag cggcttccgc
      781 gaccgcttcg agctcgtgac ccgatgggcg gccgagccgc tcgatctgct ccgcgagctt
      841 cgcaagctca agccgacagt ggtccacttc agcggccacg gcacggcagg cgtcgccggc
      901 cacgcttccg ggacgaagcg tcatcgcgat gtcgacatcg agcccggcca caacggcggc
      961 gatccctgct ccggactgct cttccaggac gccgatgggg gcccgcagct cgtgtcgacc
     1021 gaggcgctcg aggcgacatt cggcgcggcg ggatcctcgg tccagctgac cgtcctgagc
     1081 gcctgctaca gcgagctgaa tgcccaggcg ctattgcagc acgtcgactg tgtcgtcggg
     1141 atgagcggcg cgatcgccga cgacgcggca cggagcttcg cgatcggctt ctacggcggc
     1201 ctcggcgagc gcgagtcgat cgccgccgcg tacgcgcagg ggtgcgctgc gatcagcctc
     1261 caggggcttt ccgatcgcga tcgccccaag ctcgccgtcc gcaccggcat cgacgccggc
     1321 aagctcgtcc tggccgcgta cgcgcccgat cgagctgccg gtcgcggcgg ctaggctgcg
     1381 gcggctgaga tcccacgtgg gagcgggatg tcttggtcgg ctgtacgggg ttgcagccgg
     1441 aggctggcac atgctggcat ccacgtgggc gttgcggtct gccgcggtgg cgcggtgccc
     1501 ggacgattcc gcggaatcgt ggtcggcgcc cctgaacggc tgccatggcg agcagatccg
     1561 aagcggagtc ttcgaggcgc gatcgcggga cgtcaacgcg agctcgccgg gggcgaccgc
     1621 gagcaggatg gggtcggacg ccgcgaccgt gaccaggact ggccggtctt gccccgcaca
     1681 taccaagggc tgtccgcgca ggcggcggtg gtgattcggc gggcgcggct ccgactcccc
     1741 atccgcgcag cagcccccgg cgagctcgcg tgtgcgcgtt ctccccggag ctccgcgcga
     1801 cccactccta cccgccggcg agcggggcga gcagatagat ctcgtcgccg tcggcgagca
     1861 cgcggtcctc gttggcgtgc agcgggcagc gctcgccgtt gagcatcatg gtatcggtca
     1921 ggtgcggcga gtgggcgagc gcctcgcgca gcggcagccg cgcggcgtcg gtgatgtcca
     1981 gcagcttgcc gagcgtggtg ccggccggga cgcggacgta ctcgtcgatg tccttccagt
     2041 cgtcgccgat ccggcccttg atcaggatgt ggatccggac gttgccgccg cgcagccggt
     2101 cgaagatgcc catgccgccg cggcctactc caggtagccg tcgagcaggt cggccatgcc
     2161 gagctcggcg gcgcgcgacc gcgcgaggtg gccggtggtg gtgttccagt gcatcgactt
     2221 gtagtagtcg tcgcgcagca ccggcagcgg gacccggatg cccttgagcg ggccggcctc
     2281 gagctggcca tcgccctcgc cgagcatgcg gcggtggggc tggaagtcct tgggcgcgag
     2341 gccctcgcgg cggttgaacg cggcgcgcag gttctggatc cgctcgccgc a
//
LOCUS       DASVOR010000676         2266 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_562044,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000676 DASVOR010000000
VERSION     DASVOR010000676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562044"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="VF516_13325"
     CDS             <1..332
                     /locus_tag="VF516_13325"
                     /inference="COORDINATES: protein motif:HMM:NF036804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YchJ family metal-binding protein"
                     /protein_id="HEX8108707.1"
                     /translation="PSTRSSIDRAGITDWSRQTEWLGLEILDTVRGGEDDDDGIVEFI
                     ARGRTRGAPFAHRERSRFRRRDGVWYYVDGAQPREPARAAAAVGRNDPCPCGSGKKYK
                     RCHGAIP"
     gene            402..1112
                     /locus_tag="VF516_13330"
     CDS             402..1112
                     /locus_tag="VF516_13330"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HEX8108708.1"
                     /translation="MRTLLSLLILSMLFTAPGRAAPPDTHPMKIGIIGAGHIGGALAK
                     LWVAAGHEVLLSSRHPDELKPLAKQLGPRARVGTPREAASFGDVVLVSVPYKALPELG
                     RDLKAELAGKIVLDTCNPYPGRDGDMANEARKKGTGVADPEFLPGTRLVRAFNAINAG
                     SLAGEAHRKPPLVGIPLAGDDAEALRVAQRLVTDAGFDPVVVGGLARARDFDVGTPVY
                     TKLLTAAELRKALGPSKP"
     gene            1181..>2266
                     /locus_tag="VF516_13335"
     CDS             1181..>2266
                     /locus_tag="VF516_13335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108709.1"
                     /translation="MVPERYGSYRVYEQLGKGGMATVHRAEQDTPRGVRQVALKRLVP
                     TLDRRSVALFLDEARLVRYLQHPNIPETYDSGRVFGTYFIAMEYIPGRTLKDLVAHCR
                     TTVGPVPQPIVLDLAMQLCDALDYAHKRRDEHGRPLGIIHRDVTPSNLILAPTGVLKL
                     VDFGLAKARPATEPSVAGVIKGKLGYVAPEYTRGEIDHRADLWAVGIIMYELLTGGRQ
                     LFEAPDAVDTLSRVRELPIPRPSLGNPDVHPDIDDVVMSALERDPRRRWPSAASMRDR
                     IRAVSARLGDPADDRRVIDWVNWAFDQKQGRAAQLTPMMPMPVYTAAATTDPAPTPAP
                     LPPAPRRSLGPVLALVAVILALGALGWV"
BASE COUNT          321 a          855 c          792 g          298 t
ORIGIN      
        1 atccgtcgac ccgcagctcg atcgatcgcg ccgggatcac cgactggtcg cgccagaccg
       61 agtggctcgg gctcgagatc ctcgacacgg tgcgcggcgg cgaggacgac gacgatggca
      121 tcgtcgagtt catcgcccgc ggccgcacgc gcggggcacc gttcgcgcac cgggagcgct
      181 cccggttccg gcgccgcgac ggcgtctggt actacgtcga tggtgcccag ccgcgcgagc
      241 cggcgcgcgc cgcggcggcc gtcggccgca acgatccgtg tccgtgcggc agcgggaaga
      301 agtacaagcg ctgccacggc gcgatcccgt aggtcacgcg cagcgcccgc gcagcggaca
      361 ggacgccgag ccggtgacat ccttctggcc ggaggccatc gatgcgcacc ctgctttcgc
      421 tcctgatcct gagcatgctg ttcacggcgc ccggccgcgc cgcgcccccg gatacgcacc
      481 cgatgaagat cgggatcatc ggcgccggac acatcggcgg ggcgctcgcc aagctctggg
      541 tcgccgccgg gcacgaggtg ctgctgtcgt cccgccatcc cgacgagctc aagccgctgg
      601 ccaagcagct cggccccaga gcgcgggtcg ggacccccag ggaggcagcg agctttggcg
      661 acgtcgtcct ggtctcggtc ccgtacaagg cgctgcccga gctcgggcgc gatctcaagg
      721 ccgagctcgc gggcaagatc gtcctcgaca cctgcaaccc ctacccgggg cgcgacggcg
      781 acatggccaa cgaggcgcgc aagaagggga ccggggtcgc ggatccggag ttcctgcccg
      841 gcacgcgcct ggtccgcgcc ttcaacgcca tcaacgcggg cagcctcgcc ggcgaggcgc
      901 atcgcaagcc gccgctcgtc ggcatcccgc tcgccggcga cgacgccgag gccctgcggg
      961 tcgcccagcg cctggtgacc gacgccgggt tcgatcccgt ggtggtcggc ggcctggcgc
     1021 gcgcccggga cttcgatgtc gggacgccgg tctacaccaa gctcctcacc gccgcggagc
     1081 tgcggaaggc gctcggcccg tcgaagccgt aactcccgac ccgcttccgc cgggccagca
     1141 gctccggagc cggccgcggc tccgatacaa tggaggcgag atggttccgg agcgctacgg
     1201 ctcgtatcgg gtgtacgagc agctcggcaa gggcgggatg gccacggtgc accgcgccga
     1261 gcaggacacg ccgcggggcg tgcgacaggt cgcgctcaag cgactggtgc cgacgctcga
     1321 ccgcaggtcc gtggcgctgt tcctcgacga ggcccggctg gtccggtacc tgcagcaccc
     1381 gaacatcccc gagacctacg actcgggccg ggtgttcggg acctacttca tcgcgatgga
     1441 gtacatcccg gggcggacgc tcaaggacct cgtggcccac tgccggacca cggtcggccc
     1501 ggtgccccag ccgatcgtgc tcgacctcgc gatgcagctg tgcgacgcgc tcgactacgc
     1561 gcacaagcgg cgcgacgagc acggccggcc gctcggcatc atccatcgcg atgtcacgcc
     1621 ctccaacctg atcctcgccc cgaccggcgt cctcaagctg gtcgatttcg gcctcgcgaa
     1681 ggcccggccc gcgacggagc cgagcgtcgc cggcgtgatc aagggcaagc tcggctacgt
     1741 cgctccggag tacacgcgcg gcgagatcga tcaccgggcc gatctgtggg cggtcggcat
     1801 catcatgtac gagctgctca ccggcggccg ccagctgttc gaggcgcccg acgccgtcga
     1861 cacgctgtcg cgggtgcgcg agctgccgat cccgcggccc tcgctcggca acccggacgt
     1921 ccatcccgac atcgacgacg tcgtgatgag cgcgctcgag cgcgatccgc ggcgccgctg
     1981 gccctcggcg gcttcgatgc gcgaccggat ccgcgcggtg agcgcccggc tcggcgaccc
     2041 ggccgacgac cggcgcgtca tcgactgggt caactgggcg ttcgaccaga agcagggccg
     2101 cgcggcccag ctgaccccga tgatgccgat gccggtctac acagccgccg cgacgacgga
     2161 tccggccccg acccctgcac cgttgccgcc ggcgccccgg cgcagcctcg gcccggtcct
     2221 cgccctggta gcggtcatcc tcgcgctcgg cgccctcggg tgggtg
//
LOCUS       DASVOR010000677         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_269356,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000677 DASVOR010000000
VERSION     DASVOR010000677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269356"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VF516_13340"
     CDS             complement(<1..747)
                     /locus_tag="VF516_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108710.1"
                     /translation="MGNEVAKGSAAAQIIAEEERLYGQVQARVAFGEDDDGRPQVGAG
                     DLDADLISLRDQIAEARPEDLPPLVEQMTRLAALRARLGGGKQLPVDITSPYFAHIRL
                     REHGKTKDVLVGKRGFIDRQSNVQIVDWRNAPVSRIYYRYEEGDDYEEEFAGRRVEGL
                     VEVRRNVSIARSNLRRIGAPQGTYLKDARGSWVQAVGQLAPVLHGGRGKAARPVLASG
                     TKGTLGIHHGVARADKALPEIAALIDKEQFD"
     gene            901..>1987
                     /locus_tag="VF516_13345"
     CDS             901..>1987
                     /locus_tag="VF516_13345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108711.1"
                     /translation="MTAVAPQLPLGSRLGSYELIRLIAQGGMADLYLARRVGPGRFER
                     HVAVKVLNRMRAADPEACAMFLDEARLVALLNHHNIASVFEVDVVDGKHYLAMEYVHG
                     ADLREVLARSQGAGELLPFETALSIVCAAATGLDHAHRRRGPDGAPLRLVHRDVSLSN
                     IMVGHDGSVKVVDFGIATTTIASVHTLPGVVRGKASYMSPEQCLGDPVDQRCDVFALG
                     VVLYELTTGARCFAGKTDFERMLAVVHGDYLAPSDLVASYPAELEAVIRTALATDPRQ
                     RYASAAALVEALQRVAVSQGWIGGQGAIQRAMRELFGEVTEPWTSQIDDAPVTEPHSI
                     VSFYASSPASLPRIAEPTRPARRLARGT"
BASE COUNT          261 a          707 c          711 g          308 t
ORIGIN      
        1 atcgaactgc tccttgtcga tcagcgcggc gatctcgggc aacgctttgt cggcgcgcgc
       61 gaccccgtgg tggatcccca gcgtgccctt ggtgccactg gccaggacag ggcgcgccgc
      121 cttgcccctg ccgccgtgca ggaccggcgc gagctggccg accgcctgga cccacgagcc
      181 gcgcgcgtcc ttgaggtagg tgccctgggg cgcgccgatc cggcgcaggt tggagcgggc
      241 gatcgagacg ttgcggcgga cctcgaccag gccctcgacc cgccggccgg cgaactcctc
      301 ctcgtagtcg tcgccctcct cgtagcggta gtagatccgc gacaccggcg cgttgcgcca
      361 gtcgacgatc tggacgttgg actggcgatc gatgaagccg cgcttgccga ccagcacgtc
      421 cttggtcttg ccgtgctcgc ggagccggat gtgggcaaag tacggcgaag tgatgtcgac
      481 cggcagctgc ttgccgccgc cgagccgggc gcgcagcgcc gcgagccgcg tcatctgctc
      541 gaccagcggc ggcaggtcct cgggccgggc ctcggcgatc tggtcgcgca gcgagatcag
      601 gtcggcgtcg agatcgccgg cgccgacctg cggccgcccg tcgtcgtcct cgccgaacgc
      661 gacccgggcc tggacctggc cgtacaggcg ttcctcctcg gcgatgatct gcgccgccgc
      721 gctgcccttt gcgacttcat tgcccatgcc tggaacgggc gaatctaacc cagtcggccc
      781 gggccgcaag cgtgtaagtt cgctcacgcc ggctgcccgg gagttacgtg caagtgaccg
      841 gatccgccgg gccctcggat cccgggaatc tattcgatgt tgtcgatcgt aggatgtgcg
      901 atgacagccg tcgccccaca gctcccgctc ggaagccggc tgggcagcta cgagctgatc
      961 cggctgatcg cgcagggcgg gatggccgat ctctacctgg cgcgccgcgt cggaccgggc
     1021 cggttcgagc gccacgtggc ggtcaaggtg ctgaaccgga tgcgggccgc cgaccccgag
     1081 gcgtgcgcga tgttcctgga tgaggcgcgg ctggtcgcgc tgctcaacca ccacaacatc
     1141 gcctcggtgt tcgaggtcga cgtggtcgat ggcaagcact acctcgcgat ggagtacgtg
     1201 cacggcgcgg acctgcgcga ggtgctcgcg cgcagccagg gcgcgggcga gctgctgccg
     1261 ttcgagaccg cgctgtcgat cgtgtgcgcc gcggcgaccg ggctcgacca cgcccatcgc
     1321 aggcggggcc cggatggggc gccgctgcgg ctggtccacc gcgacgtgtc gctgtccaac
     1381 atcatggtcg gccacgacgg ctcggtgaag gtggtcgatt tcgggatcgc gaccacgacg
     1441 atcgcctcgg tccacaccct gcccggcgtg gtccgcggca aggcgagcta catgtctccg
     1501 gagcagtgcc tgggcgaccc ggtcgaccag cgctgcgacg tgttcgcgct cggcgtcgtg
     1561 ctctacgagc tgaccaccgg cgcgcggtgc ttcgccggca agaccgactt cgagcgcatg
     1621 ctcgcggtgg tccacggcga ctacctcgcg ccgagcgatc tggtcgcgag ctacccggcg
     1681 gagctcgagg cggtgatccg cacggcgctc gccaccgacc cgcgccagcg ctacgcgtcg
     1741 gcggccgcgc tggtcgaggc gctccagcgg gtggcggtca gccagggctg gatcggcggt
     1801 cagggcgcga tccagcgcgc gatgcgcgag ctgttcggag aggtcacgga gccgtggacg
     1861 tcgcagatcg atgacgctcc cgtcaccgag ccgcactcga tcgtctcgtt ctacgcctcg
     1921 tcgcccgcct cgttgccgcg gattgccgag ccgacccgcc cggcgcgccg gctcgcccgc
     1981 ggcaccg
//
LOCUS       DASVOR010000678         7697 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_474213,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000678 DASVOR010000000
VERSION     DASVOR010000678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7697
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474213"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1290)
                     /locus_tag="VF516_13350"
     CDS             complement(<1..1290)
                     /locus_tag="VF516_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEX8108712.1"
                     /translation="MDRTPLNPEQRGAVEHGDGPLMVLAGAGTGKTRVLVHRIARLVE
                     TGTPPWQILAVTFTNKAAGEMRYRLRELLGEGADAMWIGTFHATCARLLRRFGEHVGL
                     TRSFVIFDDDDQIKLVEKLLKETGLDEAVSPRTVLSRIDRAKNRGLDPRTPTGSFDDV
                     IEKVYPLYQAQLAKENAVDFNDLLLKTIELFKFESAARVLTVKFRHVLVDEFQDTNKV
                     QYQLVWRFAEATRNLTVVGDDDQSIYAWRGAEPRNLLDFDRDFPDARVIKLEQNYRST
                     QTILDAANGIIRKNRDRHEKSLWTDRSGGEPIEIYQAGDERGEAYFVAQSIRRLLDDG
                     PASPSEIAILYRTNAQSRVLEEHLRAARVPAKVVGAVSFFERKEIKDVIAYLRLLANP
                     AADSAFERVVNVPARGIGDTTVDRLRAAARAHGTGLLA"
     gene            complement(1333..4740)
                     /locus_tag="VF516_13355"
     CDS             complement(1333..4740)
                     /locus_tag="VF516_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8108713.1"
                     /translation="MTGMTARHLVIAAALAGVAIALPASPAAAQSTTTGAILGRVVDA
                     DTGENLAGVTVVVTSPALQGTQTAISDENGAYKITGLPPGSYLVTFYINQLTVQHPDV
                     NVGLDRTTQLFEKLKLSQAPGEIVKVTGSAPTIDPTSTTQGITIDKNYLHNIPVPGRT
                     FDTALGAAAGSQGDGLGVAFSGSSSLENQYYVDGVNITGPTFGTVGTPVINDFIQEIE
                     VVTGGYNAEYGRATGGVVNVVTKTGSNELRGSIFGTVQPGFLTAAAQSTPINATSIDQ
                     VANNAYIADLGFDLGGPIIKDRLWFYVGFAPQLARTDFTRTTKRQTDCHVVLPSGQES
                     LCDPRPVAMGGFADGRPDIDPRTGFFLTDTLDSEIRSSTVQIYNTIGKLNFAATPENQ
                     LQLTLQALPRFATNPGIFGLEDTGFKAQELTTDLGLKWTSKLDDGRTEIEAVAGWHHD
                     HLETEAMAPILAGQPLQILSGGNLGVWAPGFGGESQQTIAGCADNKAGDPYPDIVNCP
                     MVTRPYLVGGPGPMLNDTEQRLTALAAVTQRVKAFGNHEIKAGVDIDADTADKARLYT
                     GGGLIQNAIGNDIIVTRWVQLKGRQDSPQAMNNTDPRFDNKCSTPSSGGSATATSTTY
                     LCDYLGGDQGDPGTQVTSNTLNWAAYLRDSWQVMPNLTVNAGLRYEEQRLRYATFLQH
                     TTDPLTDVPLGKNAMTLQNLWAPRIGAIYDWTREGRSKVYANWGRFYESIPLDINARS
                     FGGEVQLVQHFSPPGGPMTKCGGFDPRFGGSNGSDCLTSSSATASQEQLIGASGVLIA
                     PGIRPQYLDEFVVGAEYEVIDDLKVGLSYQNRRLGRVVEDVSPDGANTFIIANPGEWS
                     SDEERKLEALIARTDDMATKTRLQNELTLFQGIRIFDKPHRDYNALQLTVTRRFSRQL
                     YLQGSYTYSRVTGNYPGLISYDNAQIDPNISTQYDLIELLANRQGPLPQDRPHYVKLD
                     GYYTFDFHKQGALTLGARIRAFSGIPKNALAAHYLYGANESFLLPRGALGRTDLEHGI
                     DLRAGYSRDIGRGMTMEVFIDVYNLYDYQGSANVDTTYAPANTITGQPQAANPVSGGS
                     YEDLIWVKRVDTRGNETSAPIGRNPNFGNTASRYAPAYGRLGARLTF"
     gene            complement(4753..5955)
                     /locus_tag="VF516_13360"
     CDS             complement(4753..5955)
                     /locus_tag="VF516_13360"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8108714.1"
                     /translation="MALRWITHRAPWAVAACLVVGLVTACSDPTSATDLHPEGPPMVE
                     QVRMEETIGPGQFRVVFGFGTHPDATPDVEHPVTAATASGNRFRIIMDELLRGNNLEE
                     IECQGVVDTDQFARVPLGATPDDIARCTVGRDLLPSRCPASNPRSLCICANDAGCAGT
                     DANKQSFMTPKGESVGVRDDDHDGAADDTRFIQGAVGIRCGAIDVPIDLDSSYWNPSG
                     DQQTPAQGGFDALGPAVVLVPGPGTDVALPTNVDCGLVFSPDVVDKDGNQVCAPPSGD
                     ISAGCTPGDTSAIRFHVEPMTLAAASPTNPPPLRMADFLIQANAPIDPATLANITVTQ
                     DPDTSYTDFTATIAPSIPNQIKIHWTAAGGLAPSTQYTITVPTTVTDRYHQAPLQPLQ
                     IAFTSAAN"
     gene            complement(6155..6622)
                     /locus_tag="VF516_13365"
     CDS             complement(6155..6622)
                     /locus_tag="VF516_13365"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8108715.1"
                     /translation="MGMSSGSGDKKSVQSEINVTPLIDVLLVLLIIFLVVMPIMMKME
                     MLEIPRKISDNEAPDPNATMLTIKVKASGDMVFTDNDKETSINAADLLRTLRPKLEAA
                     QQRGVERVVFVDFDDGVLWANVVSTMDSIRALAADVNHDEIKVALKLKEDKVQ"
     gene            complement(6625..7113)
                     /locus_tag="VF516_13370"
     CDS             complement(6625..7113)
                     /locus_tag="VF516_13370"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8108716.1"
                     /translation="MSGQVGHAHRRWRLSGHKKAHIDVVRNDINVTPLVDVMLVLLII
                     FMLMTLIMGRGHDVILPKAANYSQEKDKMQPVVSVDVDGNLWIEKTKLGPVSVSTLKE
                     MNDQVEGAWKAPKNPDGVGRIYLKADDKVEYGKIYPVLEYLNKTMAMQSVDLAIAKQE
                     DK"
     gene            complement(7116..>7697)
                     /locus_tag="VF516_13375"
     CDS             complement(7116..>7697)
                     /locus_tag="VF516_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8108717.1"
                     /translation="ERLLTFRAASDQSIRYVQALGEYLRQHKVNEALNAARSMPKSPV
                     AKVMESGLTALIQGREALKTEGPDDVGDFDLVDSVNRALERVKERETSNLRRGLAYLG
                     TVASATPFIGLLGTVVGILGTFQLLKGGSVTINEIGPKISEALVSTALGLAVAIIGAM
                     SFNFFTSRVEQYVIDMNDVSSEFIDYVLREGRS"
BASE COUNT         1071 a         2521 c         2735 g         1370 t
ORIGIN      
        1 cgcgagcagg cccgtaccgt gcgcccgggc cgccgcgcgc agccggtcga ccgtggtgtc
       61 gccgatcccg cgcgccggga cgttgaccac gcgctcgaac gccgagtccg ccgcggggtt
      121 ggcgagcagc cggaggtacg cgatgacgtc cttgatctcc ttgcgctcga agaacgacac
      181 cgcgccgacc accttggccg gcacccgcgc cgcgcgcagg tgctcctcca gcacgcgcga
      241 ctgcgcgttg gtccggtaca ggatcgcgat ctcgctgggc gacgccggcc cgtcatcgag
      301 cagccgccgg atcgactgcg ccacgaagta cgcctcgccg cgctcgtcgc cggcctggta
      361 gatctcgatc ggctcgccgc cgctgcggtc ggtccacagc gacttctcgt ggcgatcgcg
      421 gttcttgcgg atgatgccgt tggcggcgtc gaggatggtc tgggtcgagc ggtagttctg
      481 ctcgagcttg atcacccggg cgtcggggaa gtcgcgatcg aagtcgagca ggttacgcgg
      541 ctcggcgccg cgccaggcgt agatcgattg atcgtcgtcg ccgaccaccg tcaggttgcg
      601 ggtcgcctcg gcgaaccgcc acaccagctg gtactggacc ttgttggtgt cctggaactc
      661 gtcgaccagg acgtggcgga acttgaccgt caggacgcgc gccgccgact cgaacttgaa
      721 cagctcgatg gtcttgagca gcaggtcgtt gaagtcgacc gcgttctcct tggcgagctg
      781 ggcctggtac agcgggtaga ccttctcgat cacgtcgtcg aacgacccgg tcggcgtccg
      841 cgggtcgagc ccgcggttct tggcgcggtc gatccgcgac agcaccgtgc gcggcgagac
      901 cgcctcgtcg agcccggtct ccttgagcag cttctcgacc agcttgatct ggtcgtcgtc
      961 gtcgaagatc acgaagctcc tggtcagccc gacgtgctcg ccgaaccgcc gcagcaggcg
     1021 cgcgcaggtc gcgtggaacg tgccgatcca catcgcgtcg gcgccctcgc cgagcagctc
     1081 gcgcagccgg taccgcatct cgcccgccgc cttgttggtg aacgtcaccg cgaggatctg
     1141 ccacggcggc gtgccggtct cgaccagccg cgcgatgcgg tggaccagga cgcgggtctt
     1201 gcccgtgccg gcgcccgcca gcaccatcag cggaccgtcg ccgtgctcga cggcgccgcg
     1261 ctgctccggg ttgaggggcg tgcgatccac gtccggttgc ttagcacctg gttctgacag
     1321 cgggcagccc gactagaacg tcagccgggc cccgagccgg ccgtacgccg gcgcgtagcg
     1381 cgacgcggtg ttgccgaagt tcgggttccg gccgatcggg gccgacgtct cgttgccccg
     1441 ggtatcgacc cgcttgaccc agatcaggtc ctcgtaggac ccgccggaca ccgggttggc
     1501 ggcctggggc tggccggtga tcgtgttggc cggggcgtag gtggtgtcga cgttcgcgct
     1561 gccctggtag tcgtagaggt tgtacacgtc gatgaacacc tccatcgtca tgccgcggcc
     1621 gatgtcgcgg ctgtagccgg cgcgcagatc gatgccgtgc tccaagtcgg tgcgcccgag
     1681 cgcgccgcgc ggcagcagga acgactcgtt cgcgccgtag aggtaatgcg cggcgagcgc
     1741 gttcttcggg atgcccgaga acgcgcggat ccgcgcaccg agggtcagcg cgccctgctt
     1801 gtggaagtcg aacgtgtagt agccgtcgag cttgacgtag tgcgggcgat cctggggcag
     1861 cgggccctgg cggttggcga gcagctcgat caggtcgtac tgggtcgaga tgttcggatc
     1921 gatctgggcg ttgtcgtacg agatcagccc cgggtaattg ccggtgaccc gcgagtaggt
     1981 gtacgagccc tgcaggtaga gctggcggga gaaccgccgg gtcacggtga gctggagcgc
     2041 gttgtagtcg cggtgtggct tgtcgaagat ccggatgccc tggaacagcg tgagctcgtt
     2101 ctgcagccgg gtcttggtcg ccatgtcgtc ggtccgcgcg atcagcgcct cgagcttgcg
     2161 ctcctcgtcc gacgaccact cgcccgggtt ggcgatgatg aaggtgttgg cgccgtcggg
     2221 cgacacgtcc tcgaccacgc ggccgagccg gcggttctgg tacgacaggc cgaccttgag
     2281 gtcgtcgatc acctcgtact cggcgccgac cacgaactcg tcgaggtact gcggccggat
     2341 gccgggtgcg atcaggacgc ccgacgcgcc gatcagctgc tcctggctgg ccgtggcgct
     2401 ggagctggtg aggcaatcgg agccgttgct gccgccgaac cgggggtcga aaccaccgca
     2461 cttcgtcatg gggccaccgg ggggggagaa gtgctggacg agctggacct cgccgccgaa
     2521 cgaccgcgcg ttgatgtcga gcgggatcga ctcgtagaac cggccccagt tggcgtagac
     2581 cttcgaccgg ccctcccggg tccagtcgta gatcgcgccg atccgcggcg cccacaggtt
     2641 ctggagcgtc atcgcgttct tgccgagcgg cacgtcggtc agcgggtcgg tggtgtgctg
     2701 gaggaaggtc gcgtagcgca gccgctgctc ctcgtagcgc aggccggcgt tgaccgtcag
     2761 gttgggcatg acctgccacg agtcgcgcag gtacgccgcc cagttgagcg tgttgctggt
     2821 gacctgcgtc cccgggtcac cctggtcgcc gccgaggtag tcgcacaggt acgtggtcga
     2881 cgtagccgtc gccgagccgc cgctgctggg cgtgctgcac ttgttgtcga accgcggatc
     2941 ggtgttgttc atcgcctgcg gcgaatcctg ccggcccttg agctggaccc agcgggtcac
     3001 gatgatgtcg ttgccgatgg cgttctggat caacccgccg cccgtgtaca gccgggcctt
     3061 gtcggcggtg tcggcatcga tgtcgacccc ggccttgatc tcgtggttgc cgaacgcctt
     3121 gacccgctgg gtcaccgcgg ccagcgcggt gagccgctgc tcggtgtcgt tgagcatcgg
     3181 ccccgggcca ccgacgaggt acggccgcgt gaccatcggg cagttgacga tgtccgggta
     3241 cgggtccccg gccttgttgt cggcgcagcc ggcgatcgtc tgctgcgact cgccaccgaa
     3301 cccgggagcc cacacgccga ggttgccgcc gctcaggatc tgcagcggct ggccggccag
     3361 gatcggagcc atcgcctcgg tctccaggtg atcgtggtgc cagcccgcca ccgcctcgat
     3421 ctcggtccgg ccgtcgtcga gcttcgacgt ccacttgagg ccgaggtcgg tggtcagctc
     3481 ctgcgccttg aacccggtgt cctcgaggcc gaagatcccc gggttggtcg cgaaccgggg
     3541 cagcgcctgg agcgtgagct ggagctggtt ctccggggtc gcggcgaagt tgagcttgcc
     3601 gatcgtgttg tagatctgca cggtgctgct gcggatctcg gagtcgagcg tgtcggtgag
     3661 gaagaacccg gtcctgggat cgatgtcggg acgcccatcg gcgaagcccc ccatggccac
     3721 ggggcgcggg tcgcacagcg attcctggcc gctcggcagc accacgtggc agtcggtctg
     3781 gcgcttggtg gtccgggtga agtcggtccg ggcgagctgg ggggcgaacc cgacgtagaa
     3841 ccacagccgg tccttgatga tcgggccgcc gagatcgaac ccgagatcgg cgatgtacgc
     3901 gttgttggcc acctggtcga tcgacgtcgc gttgatcggc gtggactgcg ccgccgcggt
     3961 caggaacccg ggctggaccg tcccgaagat cgagcccctg agctcgttcg atccggtctt
     4021 ggtcacgacg ttgaccacgc cgccggtcgc gcggccgtac tcggcgttgt acccgccggt
     4081 gacgacctcg atctcctgga tgaagtcgtt gatcaccggc gtgccgaccg tgccgaacgt
     4141 cgggccggtg atgttgacgc cgtcgacgta gtactggttc tccagcgacg agctgccgga
     4201 gaacgcgacg cccaggccgt cgccctgcga gccggccgcg gcgccgagcg cggtgtcgaa
     4261 cgtccggccc ggcaccggga tgttgtgcag gtagttcttg tcgatcgtga tgccctgggt
     4321 ggtcgatgtc ggatcgatcg tcggtgcgct gccggtgacc ttcacgatct cgccgggcgc
     4381 ctgcgacagc ttgagcttct cgaacagctg cgtggtccgg tcgagcccga cgttgacgtc
     4441 ggggtgctgg acggtgagct ggttgatgta gaacgtgacc aggtagctgc cgggcggcag
     4501 cccggtgatc ttgtacgcgc cgttctcgtc ggagatcgcg gtctgggtgc cctgcaacgc
     4561 cggagaggtc accaccaccg tgacgccggc gaggttctcg ccggtgtcgg cgtcgaccac
     4621 gcggccgagg atcgcgccgg tcgtggtcga ctgcgcggcg gcgggcgacg cgggcagcgc
     4681 gatggccacg ccggcgagcg ccgctgcgat cacgagatgc ctggctgtca tgccggtcat
     4741 gcggtcctcc gatcagttcg cggccgaggt gaacgcgatc tgcaagggtt gcagcggcgc
     4801 ctggtggtag cggtcggtca ccgtggtcgg gaccgtgatc gtgtactgcg tgctgggggc
     4861 cagcccgccg gccgccgtcc agtggatctt gatctggttc gggatggacg gtgcgatcgt
     4921 cgcggtgaag tcggtgtagc tcgtgtccgg atcctgggtg acggtgatgt tcgcgagtgt
     4981 ggccgggtcg atcggcgcgt tggcctggat cagaaagtcg gccatgcgca acgggggcgg
     5041 gttggtcggc gacgcggccg cgagcgtcat cggctcgacg tggaagcgga tcgcgctggt
     5101 gtcgcccggg gtgcagccgg cggagatgtc gccgctcggc ggcgcgcaga cctggttgcc
     5161 gtccttgtcg accacgtcgg gtgagaacac gagcccgcag tcgacgttgg tcggcagcgc
     5221 gacgtccgtg cccgggcccg ggaccagcac gaccgcgggg ccgagcgcgt cgaacccgcc
     5281 ctgcgccggc gtctgctggt cgcccgacgg gttccagtag ctgctgtcga ggtcgatcgg
     5341 cacgtcgatc gcgccgcacc ggatcccgac agcgccctgg atgaaccggg tgtcatccgc
     5401 ggcgccgtcg tggtcgtcgt cgcgcacgcc gaccgactcg cccttgggcg tcatgaacga
     5461 ctgcttgttg gcatcggtcc cggcgcagcc ggcgtcgttg gcgcagatgc agagcgagcg
     5521 cgggttgctg gccgggcagc gcgacggcag caggtcccgc ccgaccgtgc agcgcgcgat
     5581 gtcatcgggc gtcgcgccga gcggcacgcg ggcgaactga tcggtgtcga ccacgccctg
     5641 gcactcgatc tcctcgaggt tgttgccgcg cagcagctcg tccatgatga tgcggaaccg
     5701 gttgccggaa gcggtcgcgg cggtgaccgg gtgctcgacg tcgggcgtgg cgtcggggtg
     5761 ggtcccgaag ccgaacacca cgcggaactg ccccgggccg atggtctcct ccatccgcac
     5821 ctgctcgacc atcggcggcc cctcgggatg caggtcggtc gcgctggtcg ggtccgagca
     5881 ggcggtcacg aggccgacca cgaggcaggc ggccacggcc cagggtgccc ggtgcgtgat
     5941 ccagcgcagc gccatgtcca tcgtgttgcg tccctttccg gacgggtgcc cgcccgcggc
     6001 ggcgcacccg acacgagatt ctatcaaggt cgcaactcga aggggtatcc tccgcgaatc
     6061 ggccgctaca ccgttcgcgg atcacgcggc atgaccgccg cggcccgggt gtgatcgacg
     6121 tcgcgcgcgc cggacaggcg cacgcccgcc gcggtcactg caccttgtct tccttgagct
     6181 tgagcgcgac cttgatctcg tcgtggttga cgtcggccgc gagcgcacgg atcgagtcca
     6241 tcgtcgacac cacgttcgcc cacagcacgc cgtcgtcgaa gtcgacgaac accacgcgct
     6301 cgacgccgcg ctgctgggcg gcctccagct tggggcgcag cgtgcggagc aggtcggccg
     6361 cgttgatcga cgtctccttg tcgttgtcgg tgaacaccat gtcgccgctg gccttcacct
     6421 tgatggtgag catcgtggcg ttgggatcgg gcgcctcgtt gtccgagatc ttgcgcggga
     6481 tctccagcat ctccatcttc atcatgatcg gcatcacgac caggaagatg atgaggagca
     6541 cgaggagcac gtcgatcagc ggcgtcacgt tgatttcgct ctggacgctt tttttgtccc
     6601 cggagcccga tgacatcccc atggctactt gtcctcttgc ttggcgatcg cgaggtcaac
     6661 cgactgcatc gccatcgtct tgttgaggta ctccagcacg gggtagatct tgccgtactc
     6721 gaccttgtcg tcggccttga ggtagatccg gccgaccccg tcggggttct tcggcgcctt
     6781 ccacgcaccc tcgacctggt cgttcatctc cttgagcgtg gacaccgaca ccggaccgag
     6841 cttggtcttc tcgatccaga ggttgccgtc gacgtcgacc gacaccaccg gctgcatctt
     6901 gtccttctct tgcgagtagt tggccgcctt ggggaggatg acgtcgtgac cgcggcccat
     6961 gatcagcgtc atgagcatga agatgatgag cagcaccagc atgacgtcga cgagcggcgt
     7021 cacgttgatg tcgttgcgca cgacatcgat gtgcgccttc ttgtgccccg agagccgcca
     7081 gcggcggtgg gcatgtccga cctgcccgga cattgctacg accggccctc acgcagcacg
     7141 tagtcgatga actcgctgga cacgtcgttc atgtcgatca cgtattgctc gacgcgcgag
     7201 gtgaagaagt tgaacgacat ggcgccgatg atcgcgaccg cgagaccgag cgcggtcgac
     7261 accagcgcct cggagatctt cgggccgatc tcgttgatcg tcacgctgcc gcccttgagc
     7321 agctggaacg taccgaggat gccgacgacg gtgccgagca gaccgatgaa cggcgtcgcc
     7381 gacgcgaccg tgccgaggta ggccaggccg cgccgcaggt tcgaggtctc gcgctccttg
     7441 acgcgctcga gcgcgcggtt caccgagtcg acgaggtcga agtcaccgac gtcgtcgggg
     7501 ccctcggtct tgagcgcctc gcggccctgg atcagcgcgg tcaggccgga ctccatgacc
     7561 ttggcgaccg ggctcttcgg catcgaccgc gccgcgttga gcgcctcgtt caccttgtgc
     7621 tgtcgcaggt actcgccgag cgcctgcacg tagcggatgc tctgatcgct cgccgcgcga
     7681 aacgtcagga gccgttc
//
LOCUS       DASVOR010000679         2530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_532651,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000679 DASVOR010000000
VERSION     DASVOR010000679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2530
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532651"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..850)
                     /locus_tag="VF516_13380"
     CDS             complement(<1..850)
                     /locus_tag="VF516_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108718.1"
                     /translation="MLNLTEYRKRPALLADWLPWAGMVAPGVVLNKDGSFQRTARFRG
                     PDLDSATQGELVATSARLNNALRRLGSGWALFVDAVRREAADYPDSSFPEPLSWLVDE
                     ERRAAFEEDASHFESSYHVTLLYLPPEESTARAARMLYENHRSEGVDWRERLEAFVSE
                     TERLFGLLDGVMPEISWLDDAQTLTYLHGCVSTRRHAVAVPEVAMHLDALLADEPLVG
                     GLAPMLGNQHLRALSIRGFPTSTWPGLLDDLNRLGFAYRWSTRFLCLDKAESEKELSR
                     LRRQWFA"
     gene            complement(858..1130)
                     /locus_tag="VF516_13385"
     CDS             complement(858..1130)
                     /locus_tag="VF516_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011002497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirB3 family type IV secretion system protein"
                     /protein_id="HEX8108719.1"
                     /translation="MNSTIGSLAGFEVPLHRSLTEPILLGGAPRTVAIANGTLAAAVG
                     LGLQLWLPGFGLWVIGHSLAVWGARLDPQFMQVFARHIKHRQLLDV"
     gene            complement(1127..1459)
                     /locus_tag="VF516_13390"
     CDS             complement(1127..1459)
                     /locus_tag="VF516_13390"
                     /inference="COORDINATES: protein motif:HMM:NF016820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HEX8108720.1"
                     /translation="MTTSRVSAKPLLYVAAAAALLLAVALPAHAAGSNMPWEAPLQSV
                     LESIQGPVARIIAVIVIISTGLALAFGDTSGGFRKLIQIVFGLSIAFAASSFFLTFFS
                     FTGGAVLA"
     gene            complement(1456..2472)
                     /gene="trbB"
                     /locus_tag="VF516_13395"
     CDS             complement(1456..2472)
                     /gene="trbB"
                     /locus_tag="VF516_13395"
                     /inference="COORDINATES: protein motif:HMM:TIGR02782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer ATPase TrbB"
                     /protein_id="HEX8108721.1"
                     /translation="MNLPDPNGMDASLERRIRMLRTAMGPEIAAALEDPEVVEVLLNP
                     DGSLWVDRLGSGREATGAAIPPAVAERIIRLVAAHVRTEVHPGAPILSAELPETGERF
                     LGVLPPVVRAPSFAIRKRALHIMTLAHYVADGVMTEEQAAFLRRAVRERLNVVIAGGT
                     STGKTTLANALLDEIAETRDRVLILEDTVELQCRSDDHVCMRTEPGVSTMADLVRATL
                     RLRPDRIVVGEVRGSEALDLLKAWGTGHPGGIATVHAGSAHGALTRLEQLVQEVSVTI
                     PRALIAEAVNVIVFIAGRGRSRRVREIARVVGHDSHGYQLDTELSFFPSLPPSNPSST
                     GDPS"
BASE COUNT          459 a          847 c          843 g          381 t
ORIGIN      
        1 ttgcgaacca ctggcggcgc aggcggctca gttccttctc cgactcagcc ttgtccaggc
       61 acaggaatcg cgtgctccag cggtaggcga agcctaagcg gttcagatcg tcgagcaggc
      121 ccggccaagt ggaggtcgga aacccgcgaa tcgacagcgc ccgcaggtgc tgatttccga
      181 gcatcggcgc cagaccgccc actaacggtt cgtcggccag cagcgcgtcc agatgcatgg
      241 ctacctcggg cacggccacc gcatgccggc gcgtggacac gcagccatgc aggtaggtca
      301 aggtctgcgc gtcgtcgagc catgagatct ccggcatcac gccgtcgagc agaccgaaga
      361 ggcgttcggt ctcggacacg aaggcttcga gccgctcacg ccaatccacg ccttcagacc
      421 ggtggttctc atagagcatc cgggccgcgc gggccgtcga ctcctcaggc ggcaggtaca
      481 gcagcgtcac gtggtagctg ctttcgaagt ggctggcgtc ctcctcgaaa gcggcacggc
      541 gttcttcgtc aaccagccac gagagcggct cgggaaagct cgaatcggga tagtcagccg
      601 cttcgcgtcg cacggcgtcg acgaacaagg cccaaccgga gccgagacgc cgtagcgcgt
      661 tgttgagccg cgccgacgtg gcgaccaact ctccttgtgt cgcgctgtcc aggtccggcc
      721 cccggaaccg tgccgtgcgc tggaacgagc catccttgtt gagcacgacg ccgggcgcca
      781 ccattccggc ccacggtaac cagtcggcca gcaacgcggg gcgcttgcgg tattcggtga
      841 gattcaacat cgtgacctca cacgtccagc aactgccggt gcttgatgtg tcgcgcgaag
      901 acctgcatga attgcgggtc caggcgagct ccccagacgg cgagcgaatg tccgatgacc
      961 catagcccga agccgggaag ccagagctgc aggcccaagc cgactgcggc ggccagcgtg
     1021 ccgttggcga tcgccacggt gcgcggtgcc ccacccagca ggatcggctc ggtcaaagac
     1081 cggtgcagcg gcacctcgaa gccggccaga cttccgatgg tggagttcat gccagcaccg
     1141 ccccgcccgt gaagctgaag aaggtcagga agaaactgga cgcggcgaaa gcgatcgaca
     1201 gaccaaacac gatctggatc aatttccgga agccgccgct ggtgtcgccg aaggctaggg
     1261 ccaggccagt cgaaatgatg acgatcactg caatgatccg tgccaccggt ccctgaatgg
     1321 attcgagcac cgattgcagc ggtgcctccc aaggcatgtt cgagccggcc gcatgcgcgg
     1381 gcagcgccac ggcgagcagc agcgccgctg cggcggcaac gtagagaagc ggttttgcgg
     1441 aaacgcgtga agtcgtcatg aaggatctcc tgtggaagaa gggttggaag gaggaagcga
     1501 cgggaagaaa gacagctcgg tatcgagctg gtagccgtgg ctgtcatggc cgacgacgcg
     1561 cgcgatctct cgcacgcggc gcgagcggcc acggccggca atgaagacga tgacgttgac
     1621 cgcctcggca atcagggcgc gcgggatggt cacgctgacc tcctgcacca gttgctccag
     1681 ccgcgtgagc gcgccgtgcg ccgagccggc atgcacggtg gcaatgccgc cggggtgtcc
     1741 agtgccccag gccttgagca ggtccagtgc ttcggagccg cgcacctcgc cgacgacaat
     1801 gcggtccggt cgcagccgca aggtggcacg caccaggtcg gccatggtgc tcacgcccgg
     1861 ctcggtgcgc atgcacacat ggtcgtcgct gcggcactgg agctccaccg tgtcttcgag
     1921 gatcagcaca cggtcgcgcg tctctgcgat ctcgtcgagc aaggcattgg ccagcgtggt
     1981 cttgccggtg ctggtgccgc ccgcgatgac gacgttcagg cgctcgcgca ccgcgcggcg
     2041 caggaacgcc gcctgctcct cggtcatcac gccatcggcc acgtaatggg ccagcgtcat
     2101 gatgtgcagc gcccgcttgc ggatcgcaaa cgacggcgcg cgcacgacgg gcggcagcac
     2161 cccaaggaag cgttcaccag tctcaggcag ctcggccgac aggatcggtg cgcccggatg
     2221 cacctcggtc cgcacgtgcg cggccaccag ccggatgatc cgttcggcca ctgcgggcgg
     2281 aatcgctgcg ccggtggcct cccggcccga gcccagccgg tccacccaaa gcgagccgtc
     2341 cgggttcagc agcacttcga cgacttcggg gtcctccaac gcggccgcaa tctccggccc
     2401 catggctgtg cgcagcatgc ggatgcgccg ctccaacgag gcgtccatgc cgttcggatc
     2461 aggcaggttc acgaggaacc tccatggcgt cggcatcggt ccccgactgg gcatccgcct
     2521 gcgtcgggtc
//
LOCUS       DASVOR010000680         1567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_182142,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000680 DASVOR010000000
VERSION     DASVOR010000680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1567
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182142"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..574)
                     /locus_tag="VF516_13400"
     CDS             complement(<1..574)
                     /locus_tag="VF516_13400"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8108722.1"
                     /translation="MKKFPDFVPPTKVSYLDRGPTLHVRRCTLQSVDDPGHEWTFDKE
                     EVRIGSMEDNDVVLGDDTVSRYHCKITQEDNGYVLVDQRSTNGTFINKVRIREAFLKP
                     GSIVSVGQSQLRFNAREEEVEITPSPSDRCAGLIGGNPRMREFYSIIEKIAPTATTVV
                     IDGETGTGKEVVAQAIHSLSPRSRNDLVVFD"
     gene            complement(675..839)
                     /locus_tag="VF516_13405"
     CDS             complement(675..839)
                     /locus_tag="VF516_13405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108723.1"
                     /translation="MKPALLLVFLALGPALAEQLPIVPRVVSDAVAQSSGTTPPAAPR
                     DAERIWLCGR"
     gene            950..>1567
                     /locus_tag="VF516_13410"
     CDS             950..>1567
                     /locus_tag="VF516_13410"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HEX8108724.1"
                     /translation="MSAGHPRRAPGTPSAEMPGAQSFLPAERTLATMRVAVATCRGCP
                     LYRHATQAVFGEGEAHARVMMIGEQPGDSEDLAGRPFVGPAGRVLDGALAAAGIPRDE
                     VYVTNVVKHFKFTRRGKRRIHDKPTQYEVAACRPWLISELELVAPDIVVVLGATAAQS
                     LLGRMFRVTRERGVERATPLAPHTFATVHPASVLRAPDEAQREEAR"
BASE COUNT          225 a          541 c          565 g          236 t
ORIGIN      
        1 agtcgaacac gaccaggtcg ttgcgcgagc gcggcgacag cgagtggatc gcctgggcga
       61 cgacctcctt gccggtgccg gtctcgccgt cgatcaccac ggtggtcgcg gtcggcgcga
      121 tcttctcgat gatcgagtag aactcgcgca tccgcgggtt gccgccgatc aggccggcgc
      181 agcggtcgga cggcgagggg gtgatctcga cctcctcctc gcgggcgttg aaccgcagct
      241 ggctctggcc gaccgagacg atcgaccccg gcttgaggaa cgcctcgcgg atccggacct
      301 tgttgatgaa cgtgccgttg gtcgagcgct ggtcgacaag gacgtagccg ttgtcctcct
      361 gcgtgatctt gcagtggtag cgcgacaccg tgtcgtcgcc gaggacgaca tcgttgtctt
      421 ccatcgagcc gatccggacc tcctccttgt cgaacgtcca ctcgtggccc gggtcgtcga
      481 ccgactgcag cgtgcagcgc cggacatgca gcgtcggacc gcggtcgagg taactgactt
      541 tggtcggcgg tacgaagtct ggaaacttct tcatgacctg cgccgtcgcc gcatcatagc
      601 cccaaaacca cgcggcgtgc cggccggtgg gtccggttcc tgcagctttg gcggcgcggc
      661 agatctaccg ccacctacct cccacacagc cagatccgct ccgcgtcgcg cggtgcggcg
      721 ggaggggtgg tgccagagct ctgcgccacc gcgtccgaca cgacgcgcgg cacgatgggg
      781 agctgctcgg cgagcgcggg gccgagcgcg aggaacacga gcaacagggc gggcttcatg
      841 cgcgagaaaa cagcatccgc cgtgccatgc gaagcgcttg cgcgtttccg cggggcccgg
      901 gcgcgggcgc cgcacgcgca cgggcatgtc gcgtgcacga tctccggaca tgtcggctgg
      961 tcatccgcgg cgtgcgccgg ggacaccctc ggcggagatg ccgggcgcac agtcgttctt
     1021 gcccgcggag cgcacgctgg ccacgatgcg cgtcgcggtc gccacgtgtc gcggctgccc
     1081 gctgtaccgc cacgccacac aggcggtgtt cggtgaggga gaagctcacg cacgggtgat
     1141 gatgatcggg gagcagcccg gcgactcgga ggatctggca ggccggccgt tcgtcgggcc
     1201 ggccgggcgc gtgctcgacg gcgcgctcgc ggcggccggg atcccgcgcg acgaggtcta
     1261 cgtgaccaac gtggtcaagc acttcaagtt cacgcggcgc ggcaagcgcc ggatccacga
     1321 caagccgacc cagtacgagg tcgccgcgtg ccggccgtgg ctcatcagcg agctcgagct
     1381 ggtcgctccc gacatcgtcg tcgtgctcgg cgccaccgcc gcgcagtcgc tgctcggccg
     1441 gatgttccgc gtcacccggg agcgcggcgt cgagcgcgcc accccgctcg cgccgcacac
     1501 gttcgcgacc gtgcacccgg cgtcggtgct gcgcgcgccg gacgaggcgc agcgcgagga
     1561 ggcgcgg
//
LOCUS       DASVOR010000681         2481 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_35691,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000681 DASVOR010000000
VERSION     DASVOR010000681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35691"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1349
                     /locus_tag="VF516_13415"
     CDS             <1..1349
                     /locus_tag="VF516_13415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108725.1"
                     /translation="FDGAPDLARSIVQRALGGQTLRGEEAAVRRADETIDVLVSAAPI
                     SDVGEAVLGCVVSFADITEQKRAAAERSRLLDSATAARVEAEAANRAKDEFLAMLGHE
                     LRNPLAPILTALELMTLRGEERWPRERQIIERHVRHVVALVGDLLDVSRITRGMVVLQ
                     RRPVAVATVIRDAVESVGALVEERGHALACNVPGDLWVDGDEGRLRQVFSNLLTNAAR
                     YTPPGGAIEVAGAARDGQVVVAVRDNGAGIPPELLPSVFDMFVQGKRTIERSQGGLGL
                     GLSIVRSLVDLHGGSVRASSAGPGRGTEIEVSLPALRELPAGGEVRAVEPAAPASGRR
                     VLIVDDNCDAADLLAEALTEAGFCTRTAYDGPSALTMAGEFAPDVALLDIGLPVMDGY
                     ELARRLRESERGRAMRLVAVTGYGQYSDQVAARTAGFDEHFIKPIDLAVLASALRR"
     gene            complement(1374..>2481)
                     /locus_tag="VF516_13420"
     CDS             complement(1374..>2481)
                     /locus_tag="VF516_13420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108726.1"
                     /translation="LNPFALTPESCINQGGSYDMRSGYCTLSGSVKPTCSGDNPADAT
                     SNCASVDYQVVACILGGFAAMSDGVGPGGLPGPSSCCGQTDYTSCGCARNASDMGSYA
                     DSCSCPGGQDACPPPDDSCGAPGAVSPSGRCDHDPPPDPPQPPQPPQPPDPGDGHLPC
                     LFCFPPGGLPLFPPFGWPLLGPIDPNEMVGPAGVAPSNQVPVDVAMPYQVLFENDGNS
                     TAQEIVVTDALPSTLDWSTLHFTAINYGGRKIVIPSGARSFSQLDLPPIDGCVLAGRG
                     SLGVNVSLGFNSSTGVITLHLTVVDTTTGDFPADAAAGILPPSTACIGGSLEYSVLPL
                     DTDAPGTVITNQASIIFDANPAIVTNQVSNTIAP"
BASE COUNT          347 a          821 c          954 g          359 t
ORIGIN      
        1 gcttcgatgg cgcgccggat ctcgcgcggt cgatcgtcca gcgcgcactg ggcgggcaga
       61 cgctgcgggg tgaggaggcc gcggtgcggc gagccgacga gacgatcgat gtcctggtgt
      121 ctgccgcgcc gatctccgac gtcggcgagg ctgtcctcgg gtgcgtcgtc tcgttcgcgg
      181 acatcacgga gcagaagcgc gcggccgccg agcgatcgcg gctgctcgac agcgcgacgg
      241 cagcacgcgt cgaggcggag gcggccaacc gcgcgaagga cgagttcctc gcgatgctcg
      301 gccacgagct gcgcaacccg ctcgcgccga tcctcacggc gctcgagctg atgacgctgc
      361 ggggcgagga gcgctggccg cgcgagcgcc agatcatcga gcgccacgtg cgccacgtgg
      421 tcgcgctcgt cggtgatctc ctcgacgtct cgcgcatcac ccggggcatg gtcgtgctcc
      481 agcgccgccc cgtcgcggtg gccacggtga tccgcgacgc cgtcgagtcg gtgggcgcgc
      541 tcgtcgagga gcgcgggcac gcgctggcgt gcaacgtgcc gggcgacctg tgggtcgacg
      601 gggacgaggg gcgcctgcgc caggtgttct cgaatctgct gacgaacgcc gcgcgctaca
      661 cgccgcccgg cggcgcgatc gaggtcgcgg gtgcggcgcg cgacggccag gtcgtcgtcg
      721 cggtgcgcga caacggcgcc ggcatcccgc ccgagctcct gccgtcggtg ttcgacatgt
      781 tcgtgcaggg caagcgcacg atcgagcgct cgcagggtgg cctcgggctg gggctgtcga
      841 tcgtgcgcag cctcgtcgat ctgcacggcg gcagcgtccg cgcatccagc gccggtcccg
      901 gccgcggcac cgagatcgag gtcagcctgc cggcgctgcg cgagctccct gccggcggtg
      961 aggtgcgagc ggtggagccg gcggcacccg cctcgggccg gcgggtcctc atcgtcgacg
     1021 acaactgcga cgccgccgac ctgctggcgg aggcgctcac cgaggccggc ttctgcacgc
     1081 gcaccgcgta cgacggtccg tcggcgctca ccatggccgg cgagttcgcc ccggacgtcg
     1141 cgctgctcga catcggcctg cccgtcatgg acggctacga gctcgcgcgc cgcctgcgcg
     1201 agagcgagcg cgggcgggcg atgcggctcg tcgcggtgac cgggtacggc cagtactcgg
     1261 atcaggtggc ggcgcgcacg gcgggcttcg acgagcactt catcaagccg atcgatctgg
     1321 cagtgctcgc cagcgcactg cgccgctgag gtcgggtggc tcgcgatccc cggctatggg
     1381 gcgatcgtgt tcgagacctg gttggtcacg atggcggggt tcgcatcgaa gatgatcgag
     1441 gcctggttgg tgatcacggt gccgggcgcg tcggtgtcga gcggtagcac cgagtactcg
     1501 agcgagccgc cgatgcaggc ggtcgacggc ggcaggatgc ccgcggcggc gtccgccggg
     1561 aagtcgccgg tggtggtatc gaccacggtg agatggaggg tgatgacgcc ggtcgagctg
     1621 ttgaacccga gagagacgtt gaccccgagc gaccctctgc cggcgagcac gcagccgtcg
     1681 atcggcggca gatcgagctg actgaacgac cgggcgccgc tcgggatcac gatcttgcgc
     1741 ccgccgtagt tgatggcggt gaagtgcagc gtgctccagt cgagggtgga gggcagggca
     1801 tcggtgacga cgatctcctg ggcggtgctg ttgccgtcgt tctcgaacag cacctgatag
     1861 ggcatcgcga cgtcgaccgg cacctggttg ctgggagcca cgcccgccgg gccgaccatc
     1921 tcgttcggat cgatggggcc caggagtggc cacccgaacg gcggaaacag gggaagccct
     1981 cccgggggaa aacagaacaa gcagggcagg tgaccgtctc cggggtcagg tggctgtggc
     2041 ggctgtggcg gctgtggcgg atccggaggc ggatcgtggt cgcagcgccc gctgggcgag
     2101 accgcgccgg gggcaccgca gctgtcgtcg ggaggcggac aggcgtcctg accaccgggg
     2161 cacgagcaag agtccgcgta ggaccccatg tccgacgcgt ttcgcgcaca gccgcaggac
     2221 gtgtagtcgg tctgtccgca gcacgacgag ggtcccggaa gcccccccgg accgaccccg
     2281 tcggacatcg cagcaaaccc gccgaggatg caggcgacca cctggtagtc gacggaggcg
     2341 cagttgctgg tcgcgtcggc cgggttgtcc ccgctgcagg tcggcttcac gctcccggag
     2401 agcgtgcagt agccgctcct catgtcgtag ctgcctccct gattgatgca gctctccggc
     2461 gtcagggcga acgggttgag g
//
LOCUS       DASVOR010000682         6771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_474234,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000682 DASVOR010000000
VERSION     DASVOR010000682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474234"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..706
                     /locus_tag="VF516_13425"
     CDS             <1..706
                     /locus_tag="VF516_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108727.1"
                     /translation="AYLAIALVAQRGEHRRLVLIALALGVAASLSHGYLTPALASGML
                     VIAVTLPRSDRLADGLLPDAALLGITALALAAFITVRTQSQYVYLDDARDLTHDLGTA
                     LPGGRLIRTNERTHAFLADLRRTSDAVTARGRRYAIVPDAAGWWAFATQRNPLDVDWW
                     IDVELGFDHPALVQRARNALDAQRGRLTMLVQKYGPDALATELVPVSDEDSSILPYLR
                     AHWREVGETTFFRLYE"
     gene            complement(741..956)
                     /locus_tag="VF516_13430"
     CDS             complement(741..956)
                     /locus_tag="VF516_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family NRPS accessory protein"
                     /protein_id="HEX8108728.1"
                     /translation="MQEDSSESVLKVVVNDEGQYSIWPIDRDTPAGWRDAGKSGTKAE
                     CLAWIEMTWTDMRPASLRAVMDRDAKT"
     gene            complement(1048..1734)
                     /locus_tag="VF516_13435"
     CDS             complement(1048..1734)
                     /locus_tag="VF516_13435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108729.1"
                     /translation="MHLERTGGNFFSEFLRLFIPGEELGYHAPRNRLAGRYAHLPVIG
                     FIRNPWDWYVSFYYHKRFKLDILRNDPALEHLDFASATRALLHLGSDSPPFNQVKQGF
                     VNLCPEVPPLESMGVTKSELQDFRDEGIGFYTWMVLRMFACNRSLDDVRFGKFENLVD
                     EIIRLLIEFEYKITDEMSATLSALKNPNPTNHDHYSKYYDDELRAQVAKQDRLIVERF
                     DYKFEATATV"
     gene            complement(1763..2878)
                     /locus_tag="VF516_13440"
     CDS             complement(1763..2878)
                     /locus_tag="VF516_13440"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108730.1"
                     /translation="MKTRLLSRSDVVEIVRKVGRDALMDAMISSLTDAFLRYDSRAVE
                     VRKRDGFNYDRSPRTLISPEVAGGLLEWMPVLELGAASTIKMVAYNPKNPARYGIPTI
                     MGTIFQHDIKTGHLLAIADGVFLTALRTGAASAIATRILAHPEASVVGVIGCGAQAVT
                     QLHALSRVMRIEKVLAYDLDPEALGSFPDRVAFIGVDVVPTDVASLERNVDVLVTATS
                     VAVGQGPVFPGRALRPHLHINAIGADVPGKTECPATVVEAAALVCPDFRAQAIVEGEC
                     QQLPEDRIGPSLPQIVKDRAHCKDYRSRLTIFDSTGFALEDKVAMELFLGYAEKFGLG
                     TDIAIESMPLDPLDPYSLETEPLMVRPSDRDFSLRRN"
     gene            3072..3935
                     /locus_tag="VF516_13445"
     CDS             3072..3935
                     /locus_tag="VF516_13445"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8108731.1"
                     /translation="MQRDDERAEQFKRDGFLILDRIIDAETVAIARRSLDRILAGQYQ
                     GDRRPPAIRSSPHPILGDSRTTHWVINGRLLDADIWSLVTRAELGCLAARLLETSAVS
                     VIEDQLLDKPPGGAPLATHQDYRYWQFSTSPRMLTCWIALDDMSSEIGTVQVIPGSHR
                     WGQQVVERNIADDFRDSVDGATSYLRVAEALRPPGAEIQFTPVIVPTGGGVLFHSLTI
                     HSSGTNSTDRRRRALSIHYASAECRIILVKTHDQAFPYCFARLKEGDRLVNKYLPEVY
                     SADPPSTRVDS"
     gene            4059..5720
                     /locus_tag="VF516_13450"
     CDS             4059..5720
                     /locus_tag="VF516_13450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108732.1"
                     /translation="MAIYAFHSAPMDVDTHVHLALLDTRFVSPFEMNRWGGPDGETRR
                     WIAAGLYPWYKHPDSYLVEVRPLWSGVVALAYSIFGRAPLPYYLTSLVVFLGFLGAVR
                     AWLRRQLPIELQSLAVCIFALDGLHAESAVWFANLHSVVGGALAMLAVMMHVRWREGW
                     KPGLWLAAGLSVLALAFSETSLGAFAFVVAFELLGSTANAGSRSHRIRALLPLAAICI
                     AFLALLKVGGVGAKLNPLYADPFSEPIRFLSIAAHNLRWYFEASFGGLGLGWSAVLFA
                     VSVPWFWAGMRSRSSQERRVIWAILLGAVLSLAPVLGVIQTPLEPGMFIAVRCLLYFT
                     IGLAVFWAVAVWQAATVAARAGGAAWMRIAAATATLIMILNLFVWSPVRFVGLVRYWF
                     VEDDDAATVEAIQRVPMTCRESRRVVWLRGPRSIMAFREYYLLAARLGWPSTEVGRQV
                     TVAPPGKSPLTLARTGERTIEIRSERGAVYAGDTRLNTPMPLPPGPIPVPGAEVRVLE
                     LAATQPSRIEIHFTDDPTSNCFVVQAPDGVVTRISLPEIGMETRL"
     gene            5727..>6771
                     /locus_tag="VF516_13455"
     CDS             5727..>6771
                     /locus_tag="VF516_13455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108733.1"
                     /translation="MTADGTAPVEVEAAGTASLFHRWSFWLAAIVAFAVVVITLLHTV
                     RDLPNYTGIVTSDPSGTWTGLAVDLAHGEFYRPVVSDEGFGGTRYFPLHVVLHASAIK
                     LGLGPVVAGHLISLGSLIAILVAAYLLLRRMGVQRSLSVCACTLTLGLYSQQLALIEV
                     RGDLLPAALNLWGLVVFVSNRVSNRPRLGLTAALFTLAFLAKMTTVFGAVAVALSLLF
                     AGQRKAALRFIGSMALGCAAGLLAVHVASGGRALQAITAVASGGASWVQILLRAPLLM
                     LKIVLPSDLILAVLALGTAAGLPGQERRDVLPLAVLTTGFITLVMYGSPGVDRNHLID
                     FDFIAVVLLVASGA"
BASE COUNT         1098 a         2196 c         2130 g         1347 t
ORIGIN      
        1 cgcctacctg gcgatcgccc tggtcgcgca gcgtggtgag caccggcgct tggtcctgat
       61 cgcgctcgcg ctcggcgtcg cggcctcgct ctcgcacggc tatctcacgc cggcgcttgc
      121 ctccggaatg ctggtgatcg cagtgacgct gccgcgctcg gatcgcctgg ccgatgggct
      181 actccccgac gccgctctgc tcggcatcac cgcgctcgcg ctcgccgcgt tcatcaccgt
      241 ccggacccag tcccagtacg tctatctgga cgacgcgcgg gacctgaccc acgatctcgg
      301 caccgcgctg cccggcggtc ggctgatccg cacgaacgaa cgcacccatg catttctcgc
      361 cgatctccgc cgtaccagcg acgccgtcac cgcccgcggc cgccgatacg ccatcgtccc
      421 ggacgcggcc gggtggtggg ccttcgcgac gcagcgcaac ccgctcgatg tcgactggtg
      481 gatcgacgtc gagctcgggt tcgatcatcc cgcgctggtg cagcgcgcgc ggaacgcgct
      541 cgatgcccag cgcggccgcc tcacgatgct ggtccagaag tacggtccgg acgcgctcgc
      601 cacggagctc gtgccggtga gcgacgagga ctcgtcgatc ctgccgtatc tccgcgccca
      661 ctggcgcgag gtcggcgaga ccacgttctt caggctctac gaatgatcag ggccggacgc
      721 gccctgctcg agtcgcaatt tcaggtcttc gcgtcgcgat ccatcacggc ccgcaggctc
      781 gcaggccgca tgtctgtcca ggtcatctcg atccacgcga ggcactcggc cttggttccg
      841 ctcttgccag catcgcgcca cccggcaggc gtgtctcgat cgatcggcca gatcgaatat
      901 tgtccctcgt cgttgacgac gaccttgagc acactttctg acgagtcttc ttgcatgatc
      961 tctacgctcg catgaaccag ctgcgaccac aagcctcggc tacttcaccg acggtatcga
     1021 cgccctgcgg acccttccag aatcgagtca gaccgtcgct gtggcttcga acttgtaatc
     1081 gaatcgctcg acgatgagtc gatcttgttt agccacctga gctctgagct cgtcatcgta
     1141 atacttgctg taatggtcgt gattcgttgg gttcggattc ttgagtgcac tcagtgtcgc
     1201 tgacatctca tcggtgattt tatactcaaa ttctatgagc agtcgaatga tctcatcgac
     1261 gaggttctcg aacttcccga acctcacgtc gtcgagtgag cgattgcatg cgaacatgcg
     1321 gagtaccatc caggtgtaaa agccgatacc ttcgtcgcga aaatcctgca gctcgctttt
     1381 cgttaccccc atgctctcca gtggtggaac ctcgggacac agattcacga agccttgctt
     1441 gacctgattg aaaggcggac tatcacttcc gaggtggagc agggcacggg ttgccgaagc
     1501 gaaatcgagg tgctccagag cgggatcatt tcgcaggata tcgagcttga atcgcttatg
     1561 gtaatagaag gagacgtacc agtcccacgg atttcgaatg aatccgatca ccggcagatg
     1621 agcgtagcgc ccggccaggc gattccgcgg agcatgatag ccgagctctt caccgggaat
     1681 gaacaggcga aggaactcgc tgaagaagtt accgccggtc cgctcgaggt gcaagaatac
     1741 gaacttatcc gtcgcaatca tgtcagttcc gccggaggct gaagtccctg tcagaagggc
     1801 ggaccatgag aggttcggtc tccagggaat atgggtcgag cgggtcgagt ggcatcgact
     1861 cgatggcgat gtcggtgccg agcccgaact tttccgcgta acccaggaac agttccatgg
     1921 cgaccttgtc ctcgagcgca aagcccgtcg aatcaaagat cgtgaggcga gatctgtagt
     1981 ctttgcaatg tgcccggtct ttgacgatct gcggcaggct tggcccgatc cgatcctcgg
     2041 ggagttgctg gcactcgccc tctacgatcg cttgcgcgcg gaagtccggg cacaccagag
     2101 cggccgcttc gacgaccgtc gctggacatt cggtcttgcc cggcacgtct gctccgatcg
     2161 cgttgatatg caggtgcggc cggagcgcac gaccggggaa gaccggacct tgcccgaccg
     2221 cgaccgaggt cgccgtgacc aggacgtcca cgttcctctc gagactcgcg acgtcggtcg
     2281 ggaccacatc gacgccgatg aacgcgacgc gatcggggaa cgagcctagt gcttcaggat
     2341 cgagatcgta ggcgagcacc ttctcgatcc gcatgactcg cgaaagggcg tggagctggg
     2401 tcactgcctg agctccacaa ccgatgaccc cgacgacgct cgcttcgggg tgcgcgagaa
     2461 ttcgcgtcgc tatcgcgctc gccgcgcctg ttctcagcgc ggtcaggaac acgccgtccg
     2521 cgatcgccag aagatgcccc gtcttgatgt cgtgctggaa gatcgtcccc atgatggtcg
     2581 ggatgccata gcgcgcagga ttcttcgggt tgtaggcgac catcttgatc gtcgacgcgg
     2641 ctccgagctc gagcaccggc atccattcga ggaggccgcc tgccacctcg ggtgagatca
     2701 gggttcgcgg agagcgatcg tagttgaacc cgtctcgctt tcgcacctcg accgctcgcg
     2761 agtcgtagcg aagaaacgcg tcagtcaacg aggagatcat cgcgtccatc agtgcgtcgc
     2821 gaccgacctt tcgcacgatc tcgacgacat cagaacgaga cagcagccga gtcttcatgc
     2881 gctcgggatt ctgcgcggcg gcgttccgag ctgtcaagga tgcaggacgt aaggtggcct
     2941 cgagctgccg acgtggaaca cagcttgcat agcacgagcg cgggatcggg gctggtacgg
     3001 ggctagctgc gagtggtaca tctcgggcaa gcgagcggtc acgcgcaccg gccctactac
     3061 aaggagagcc cttgcagaga gacgacgagc gggcggagca gttcaaacgc gatgggttcc
     3121 tcatcctcga tcgcatcatc gatgcggaga ccgtcgcgat cgcccggcgc agcctggatc
     3181 ggatcctggc gggtcagtat cagggcgatc gtcgcccacc cgcgatacgg tcatcgccgc
     3241 atccgatcct cggagattcg cgcacgactc actgggtgat caatgggcgc ctgctcgacg
     3301 cggacatctg gtcgctggta acgcgcgccg agctcgggtg cctcgcggcg cggttgctcg
     3361 aaaccagtgc cgtgtcggtg atcgaggatc aactcctcga taagccgcct gggggcgcgc
     3421 cgctcgcgac acatcaggat tatcgttact ggcagttctc gacaagtccg cgaatgctga
     3481 cgtgctggat cgcactcgat gacatgtcct ccgagatcgg cacggtgcaa gtgatccccg
     3541 ggtcgcaccg atgggggcag caggtcgtcg agcgcaacat cgccgatgac ttccgggaca
     3601 gcgtggacgg cgccaccagc tacctgcgtg tggctgaagc gctccgtccg cctggcgcag
     3661 agatccagtt cacgccggtc atcgtgccga ccggcggtgg cgtgttgttt catagcttga
     3721 ccattcacag cagcggaacg aacagcaccg atcggcgaag gcgagccctt tcgatccact
     3781 acgcatctgc cgaatgtcgg atcatcctcg tgaagaccca tgaccaggcg ttcccgtact
     3841 gcttcgcgcg gctgaaagaa ggcgaccgcc tggtcaacaa gtatctgccg gaggtctact
     3901 ctgccgatcc accatctacg cgcgttgact cataagttgc tgcgggatat gttatggatc
     3961 gactggtcgg tgacagctct cgatcgtcaa gtgtgatgtt gaagtcgctt ctttcgaatc
     4021 gacgcgcggt cgtagcgtgc ggtgccgcac ttgtgatcgt ggccatctac gcgttccaca
     4081 gcgccccgat ggacgtggat acgcacgtgc acctggcgct cctcgacaca cgcttcgtct
     4141 cgccattcga gatgaacaga tggggaggac ctgacggcga gacccgtcga tggatcgcgg
     4201 ccggtctcta cccctggtac aagcatcccg attcctatct ggtcgaggtt cggccgctgt
     4261 ggagcggggt ggtcgccctg gcgtactcga tattcggccg cgcaccgctg ccgtactacc
     4321 tgaccagtct tgtggtcttt ctcggtttcc tcggggccgt ccgggcgtgg ctgcggcggc
     4381 agctcccgat cgagctccag agcctcgcgg tctgcatctt tgcgctggat ggtttgcacg
     4441 ccgagagcgc cgtgtggttc gcgaacttac attccgtcgt cggcggcgca cttgcgatgc
     4501 tcgccgtgat gatgcacgta cggtggcgcg aggggtggaa gcccggactg tggcttgctg
     4561 ccgggctgtc ggtgctcgcg cttgccttca gcgaaaccag cctcggcgcc ttcgccttcg
     4621 tcgtcgcgtt cgagctcctc gggtctaccg caaacgccgg gtcacgctcg catcggattc
     4681 gcgccctcct accgctcgcg gcgatctgca tcgcgttcct ggccctgctc aaagtgggtg
     4741 gtgtcggagc caagctgaac ccgctctacg ccgatccatt cagcgagccg atccggttcc
     4801 tgtccatcgc agcgcacaac ctccgctggt atttcgaggc ctcgttcggt gggctcggcc
     4861 tcggatggtc cgctgtgctc ttcgcggtca gcgtcccctg gttctgggcc ggtatgcgct
     4921 cgcgatcctc gcaagagcgt cgcgtcatct gggccatcct cctcggcgcg gtgctttcgc
     4981 tcgcacccgt gctcggtgtc atccagactc ccctcgagcc cggcatgttc atcgccgtgc
     5041 gctgcttgct ctattttacg atcggcctgg cggtgttctg ggcagtcgcg gtctggcagg
     5101 cggccaccgt cgctgcccgc gcgggcgggg cagcgtggat gcggatcgct gcggcgacag
     5161 cgacgctgat catgatattg aacttgttcg tctggtcccc cgtacggttc gtcgggctcg
     5221 tccgttactg gttcgtcgaa gacgacgacg cggccaccgt cgaagcgatc cagcgcgttc
     5281 cgatgacatg ccgcgaatcc aggagggtcg tctggcttcg tggaccgcgt tcgatcatgg
     5341 ccttccggga gtactacctg ctggccgcgc gcctcggctg gccttccacc gaggtcggac
     5401 gccaggttac cgtggcgccg ccggggaaga gcccgctcac cctcgcgaga accggcgaac
     5461 gtacgatcga gatccgcagc gagcgaggcg ccgtctatgc gggagatacg cggttgaaca
     5521 ctccgatgcc cttgccaccc gggccgattc ccgttccggg cgccgaggtc agggtgctcg
     5581 agctggccgc gacgcagccg tcgcgcatcg agatccactt cacagacgat ccgacgtcga
     5641 actgcttcgt cgttcaggct cccgacggtg tggttacccg gatctcgtta cccgaaatcg
     5701 ggatggagac acggctgtga gaggcaatga ccgctgacgg aaccgcgccc gttgaagtcg
     5761 aggctgcagg aacggcgagc ctcttccatc gctggagctt ctggctcgcg gcgatcgtcg
     5821 cgttcgcagt ggtcgtcatc accctgctcc acaccgttcg cgatctgccg aactacaccg
     5881 gaatcgtgac cagcgatccg tcgggcacct ggaccgggct ggcggtcgat ctggcgcatg
     5941 gcgaattcta ccggcctgtc gtctccgacg agggcttcgg cgggacacgc tatttcccgc
     6001 tacacgtcgt cctccacgcg agcgcgatca agctggggct gggacccgtc gtggcaggcc
     6061 atctgatctc gctcggctcg ctgatcgcga tcctggtggc agcctatctg ctgctgcggc
     6121 gcatgggcgt gcaacgttcg ctgtcggtct gcgcgtgcac cctcaccctg ggtctgtatt
     6181 cgcaacagct cgcgctcatc gaggtgcgcg gagacctgct cccggcagcg ctgaacctgt
     6241 gggggctggt ggtcttcgtc tccaatcggg tctccaatcg gcctaggctc ggtctgaccg
     6301 cggcgctgtt cacgctcgcc ttcctggcaa agatgacgac cgtgttcggc gcggtcgccg
     6361 tggcgctgtc gttgctattc gccggtcagc gcaaggccgc cctgcgcttc atcggatcga
     6421 tggcgctcgg ctgcgccgca gggctgctgg ccgtccacgt cgcgagcggc gggcgagcgc
     6481 tgcaagcgat caccgcggtc gcctccgggg gcgcgtcgtg ggtccagatc ctgttgcgcg
     6541 ctccgctgct gatgctcaag atcgtgctgc cgtccgactt gatcctcgcg gtactcgcgc
     6601 tgggcaccgc cgccgggctc ccgggacagg aacgccggga cgtgctgccg ctggcggtcc
     6661 tcacgaccgg cttcatcacg ctggtcatgt acggctcgcc gggtgtcgat cggaatcacc
     6721 tcatcgactt cgacttcatc gccgtggtgc tcctggtcgc ctcgggcgct c
//
LOCUS       DASVOR010000683         2976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_444696,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000683 DASVOR010000000
VERSION     DASVOR010000683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444696"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF516_13460"
     CDS             complement(<1..369)
                     /locus_tag="VF516_13460"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8108734.1"
                     /translation="MIGVSQYRRIRPLPSVADAEAVAGVLRDPAACGYDPANVALLQE
                     SDATREQILDELDRLGHKAGPEATALVYFSGHGGTWPGGDAYLMPIEADGSSQDRLEA
                     TAISGRLLGAKLAAIRAERLT"
     gene            815..2194
                     /locus_tag="VF516_13465"
     CDS             815..2194
                     /locus_tag="VF516_13465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108735.1"
                     /translation="MSSEPQPSAPVSVDIVPGRLSARVHAHDIATQQARIPCWSYVTD
                     GLAAHDQPEIVFTLRREPGEAAERFPDDPLRLFATIHELAERGLRVTAGSQTEFGDAR
                     LFDQHVLYVPAQPLSGVPLPPSCLTALLVTADELRAVRTFGPTRVLARMGQAAIHYPF
                     PPWADRRRRGMAMDRTFEASALAKLPRASAQDVRVTLHGDRIVVSVLRVEPPVWPDRL
                     AQLPDRVPLALLTAFDPTANGCLVWVPGQKEPEAIIPTGSDASRVCGCFFAVFADPPA
                     NTGKILEDGFAMQLTGEAWQALRRALIEGEELTIPAADGMSCALTWRDQADDRPVEAS
                     WQASAPEPPAERPGKVALGPVRLLTSEAEFAQRTSAEQLGAFCRELQRCVERVLADRD
                     EHLELRLQVICRPRSHRVGLSHKGDASEDLLDALIDALEQVPTLPVRAEVSFEVELSV
                     ATGPRERFM"
     gene            complement(2187..>2976)
                     /locus_tag="VF516_13470"
     CDS             complement(2187..>2976)
                     /locus_tag="VF516_13470"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8108736.1"
                     /translation="AMRRALGTGFSIQFLTDEPHIPWELMRPVVDDEARDLLGVTHPV
                     ARGLQAYPDRLRPALPATGEILTIAPDYGRPPGRGLPALPGARHESAMLQQRFAAVAI
                     RSTARGLLEAFQDRRGVAVRLVHFAGHGKSEVPVDRSRIACEDDDVSVAQLRNAENRL
                     GENYGTFVVLNACESGAAGDALADVGGWGEAFAYRKFSGMIAPLWAVLDRDATAAMER
                     LFTALLHDRTPVGEALRDMRAELGSLSPTYLSYVYYGDVHARFT"
BASE COUNT          393 a         1059 c         1103 g          421 t
ORIGIN      
        1 ggtcagccgt tcggcgcgga tcgccgcgag cttggcgccg agcagccggc cggagatcgc
       61 ggtggcctcc agccggtcct gcgagctccc gtcggcctcg atcggcatca ggtacgcgtc
      121 gccgccgggc caggtcccgc cgtggcccga gaagtagacc agcgcggtgg cctcggggcc
      181 ggccttgtgg ccgagccgat cgagctcgtc gaggatctgc tcgcgggtgg cgtccgactc
      241 ctgcagcagc gcgacgttcg ccgggtcgta gccgcaggcg gccggatcgc gcagcacgcc
      301 ggccacggct tcggcgtcgg cgaccgaggg cagcggccgg atccgccggt actgagagac
      361 tccgatcaca acggcgtggg catttgtcag gctcatatca cttcccgcgt cgggtgccgc
      421 tggatcgtcc cgcggctcca tggccgatag gcgacgcacg gcggccgatc atgacagacg
      481 atcagcgctg acaggcggtg atccagggta gggatgcctg cgcgtgagcg gagcgtccgg
      541 gaatgcctgg cggggcgagt gcgttggttc gcgatgaatt tcagcacggc tgcggtgatc
      601 gtcgccgcag tccaccgacc gcgagggcgg ccggtgcggc gggcagccgc ggctggccgg
      661 cgtgtggtgg gatccgccgc gccgccgggc gctgctgctg gtcatcttcc cgggccgcga
      721 cctgtgtgaa gacgagccgg cgctgtggcg ggcctggtag cggcgcgccg gcgttgtcgt
      781 cgacaggcgt cgcgtctaag ctcgtcggac cgccatgtcg agcgaacccc agccgagcgc
      841 gccggtgagc gtggacatcg tccccggccg gctgtcggcc cgggtccacg cccacgacat
      901 cgcgacgcag caggcgcgga tcccgtgctg gtcgtacgtc accgacgggc tggccgcgca
      961 cgatcagccc gagatcgtgt tcacgctgcg ccgcgagccg ggcgaggccg ccgagcgctt
     1021 tcccgacgat ccgctccggc tgttcgcgac gatccacgag ctcgccgagc gcgggctgcg
     1081 cgtcaccgcc ggcagccaga ccgagttcgg cgacgcgcgg ctgttcgacc aacacgtgct
     1141 ctatgtcccg gcccagccgc tgtccggcgt gccgctgccg ccgtcgtgcc tgaccgccct
     1201 gctggtgacg gcggacgagc tgcgcgcggt gcgcacgttc ggcccgaccc gggtgctcgc
     1261 gcgcatgggg caggccgcga tccactaccc gttcccgccg tgggcggatc gccgccgtcg
     1321 cggcatggcc atggaccgca cgttcgaggc cagcgcgctg gcgaagctgc cgcgcgcgtc
     1381 ggcccaggac gtccgcgtca cgctgcacgg cgaccggatc gtggtcagcg tgctgcgcgt
     1441 ggagccgccg gtgtggccgg accggctggc gcagctgccg gatcgcgtgc cgctcgcgct
     1501 gctgaccgca ttcgatccga ccgccaacgg ctgcctggtc tgggtcccgg ggcagaagga
     1561 gccggaggcg atcatcccga ccggcagcga cgcgtcgcgg gtgtgcggct gcttcttcgc
     1621 ggtgttcgcc gacccgccgg cgaacaccgg gaagatcctg gaggacggct tcgcgatgca
     1681 gctgaccggc gaggcctggc aggcgctgcg ccgcgcgctg atcgagggcg aggagctgac
     1741 gatcccggcg gccgacggca tgtcctgcgc gctgacctgg cgcgaccagg ccgatgaccg
     1801 cccggtcgag gccagctggc aggcctccgc gccggaaccg ccggcggagc gccccggaaa
     1861 ggtcgcgctc ggcccggtgc ggctgttgac gtcggaggcc gaattcgccc agcggacgtc
     1921 ggccgagcag ctcggcgcgt tctgccgcga gctgcagcgc tgcgtcgagc gcgtgctcgc
     1981 cgatcgcgac gagcacctcg agctccggct ccaggtgatc tgccggccgc gcagccatcg
     2041 cgtcggcctg tcccacaaag gggacgcctc cgaggacctg ctggacgcgc tgatcgacgc
     2101 gctcgagcag gtcccgacgc tgccggtccg cgccgaggtc agcttcgagg tcgagctgtc
     2161 ggtggcgacc ggaccgcgcg agcggttcat gtgaacctgg cgtggacgtc tccgtaatac
     2221 acgtaggaca gatacgtcgg cgacagcgag ccgagctccg cgcgcatgtc gcgtaacgct
     2281 tcgccgaccg gcgtcctgtc gtgcagcaat gccgtgaaca gccgctccat ggcggcggtc
     2341 gcatctcggt cgagcaccgc ccagagcggt gcgatcatgc cgctgaactt ccggtacgcg
     2401 aacgcctcgc cccatcctcc gacgtcggcg agcgcgtcgc ctgcagctcc ggactcgcag
     2461 gcgttgagca cgacgaacgt gccgtagttc tcgccgagtc ggttctcggc gttgcgcagc
     2521 tgcgcgaccg agacgtcgtc gtcctcgcac gcgatgcgtg accggtcaac gggcacctcg
     2581 ctcttgccgt ggccggcgaa atgcaccagc cgaacggcca ccccgcggcg atcctggaac
     2641 gcttcgagca ggccgcgcgc ggtggagcgg atcgcgaccg cggcgaaccg ctgctgcaac
     2701 atcgccgatt cgtggcgcgc gccgggcaat gccggcaggc cgcgccccgg cggtcgcccg
     2761 tagtcgggtg cgatcgtcag gatctccccg gtggcaggca acgccggacg caggcgatcg
     2821 gggtaggcct gcagaccccg cgcgacggga tgcgtgacgc caagcaggtc cctggcctcg
     2881 tcgtcgacga ccggccgcat cagctcccac gggatgtgcg gctcgtcggt gaggaactgg
     2941 atcgagaagc ccgtcccgag ggcccggcgc atcgcg
//
LOCUS       DASVOR010000684        17271 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_269376,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000684 DASVOR010000000
VERSION     DASVOR010000684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17271
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269376"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VF516_13475"
     CDS             <1..465
                     /locus_tag="VF516_13475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108737.1"
                     /translation="EMGSLFATQQNIRICASQPNVTFSGTCRFPNAVTVAAANGHFHK
                     RGKSFTIFSWNGTSLDHPADTSMFYESDTWNDPPMATNLSVAEPASSGIWWDCAYQWQ
                     PPSQFTCDDVNAKDPLHQNDCCYTFGGITDVGEHCNVFLYYYPKVESDVFCL"
     gene            531..1130
                     /locus_tag="VF516_13480"
     CDS             531..1130
                     /locus_tag="VF516_13480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108738.1"
                     /translation="MLLLPAGCGGKHEAGIDAGPKCPIGDLEAPAELEIVHQDATSTV
                     LQTQSMAQIPLLPPPQGGWIVLLGARARNIDGCQVTLTTALVDRCDQQIIQIDKRPTR
                     LEPGADGWGTSTVTTFGNLPVCPELTAKRDLHDVPYDVTVVVEDSGGQKASATLPVVP
                     TCPTGDALCLCQCDRDYVIGSSCNPPPTGGHGTCPSTGP"
     gene            1372..1659
                     /locus_tag="VF516_13485"
     CDS             1372..1659
                     /locus_tag="VF516_13485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108739.1"
                     /translation="MLEVHVRTLILESLQEVIQEKPIDKQFEVSDRTKLLAKGSEMDS
                     LDYLNFSTCLEERLFRAIGREVDLSSLVVDHADAFQDVNRLAGFLAPRLAQ"
     gene            1740..2210
                     /locus_tag="VF516_13490"
     CDS             1740..2210
                     /locus_tag="VF516_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108740.1"
                     /translation="MSGGMGQERIAFDWVQVAGKTVTASCHLSDYFKSPWDFEFHLNA
                     VTGMAMISQLAMIHGMVLCGVDRKDFEILMMDYQQTMTNLIRDREGIRLSMTVVQRTV
                     VPAGWRGQHPRTFFKWTFTVNDGTWVGSIAAAFPFGPHGEGLELVRRQDGKTEP"
     gene            2210..4792
                     /locus_tag="VF516_13495"
     CDS             2210..4792
                     /locus_tag="VF516_13495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8108741.1"
                     /translation="MATLSASQALLDSLDRRRDHTALYQAGRDHTIAELLDRAGEWVA
                     YLRAAGIGPGSVCAATGEFSFNTSAFLIATLMLRCTVVPLAADSGENSQLLGLAAADI
                     VVDLDRDALHHQVVTVAKHDSRLLRDFLNTGQPGIITFTSGSAGEPKGVLHDFDRALK
                     NFGRFDRKGHRSLIFLRFNHLGGINTLLSVLASSGGVAIFPEQRTAEEIAALVARARV
                     ELLPVTPTFLAMLLASRGHERHDMSSLRLISYGAEPMPQTLLDRMNQLFPQVRFHQTY
                     GTTELCLLRTQSRSSDSLWVKLGGDGFEIRVIDGLLYVRSEFAMLGYLNAPNPIGPDG
                     WLATGDRVEQDGEWIRFLGRDSDIINVGGEKVTPLEIESVLIGAENIRDVRVYGEKDA
                     VFGARVVADVQLLTDEDPTELRRRLRRICLEKLARYKVPVKFNIVTRSFHSGRFKKVR
                     ASGPPRTSMLKTEAWVLHEGPLPVKGDRPQLPRGSQGIFPDGAFGLETLELREPTGDE
                     CLIEPLYGCWEGNFSHAIARVPVDICRQRGESKVVLGNSGVARVLRPGPDAKGLREGD
                     LCLLFGNGVADRYGYMQLAHAFDAPNTMGLLAKRANVPDRNLMPIPRDSPFTLPQWAA
                     FSLRYITAWANHRVAYGAWRLQVPEQLFPEAFPVWAWGGGVAFAQLTLARIKDSARCV
                     LIASSPARRELAARAGLETLDRSLYPELSFDASRYDADPEYAARYRDCEKRFLQEVRA
                     RTDGEGVAIFIDNIGAPVFRVTLKALAREGVVATCGWKKGMRLEILRAMECIARHTFV
                     HTHYASRQEGEDAVRFACERNWMVPRDALGKIWEWNEVAKLAEGHMTGAIDSYFPIFR
                     INPE"
     gene            4789..5565
                     /locus_tag="VF516_13500"
     CDS             4789..5565
                     /locus_tag="VF516_13500"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8108742.1"
                     /translation="MIGVITRRTHGPVVELCLDRRPANALGRAACEQWTAALRDAVTA
                     GADAIVLSGPSGVFSSGADLPELARADRSGVAAFCRALGGLVTEVAHCPVPIAAALTG
                     HCLGAASGVALLCDARFMATGHHRFRINYVAMGLVPPLYVRRAVERLVGPGQAARLLV
                     GALAVAPDEARALGLVERTAAPGEVVGAALDWCRQQLALPRTAMLETRKRLREELVAC
                     VTPENLGIDEMTDRWFGAEVQVRLAKLVTAQDGRVIANGE"
     gene            5581..6360
                     /locus_tag="VF516_13505"
     CDS             5581..6360
                     /locus_tag="VF516_13505"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF018949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8108743.1"
                     /translation="MSRIAALVYGGIAYLGFLAVTLYAIGFVGNFHVRIADGIYFVPQ
                     AVDLGRPAVPTLEALLIDGALLLLFAVQHSGMARQSAKSRLTRFISPAIERTTYVMAT
                     NVVLALLFWQWRAIGGTLWAFGDGTSAAVVLRVLNLGGWVLAFVATFLINHLELFGLQ
                     QVYAHARGAELSGPTFRQPYLYRWIRHPLYLGFLIAFWATPVMTWGHVLFSVAATGYI
                     LVGVQLEERDLIRVFGDRYREYRQRVPMLLPRPGSRQKETV"
     gene            6397..7302
                     /locus_tag="VF516_13510"
     CDS             6397..7302
                     /locus_tag="VF516_13510"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8108744.1"
                     /translation="MSVLRDLVADRHVIDVLDRSLPIAEQIGQAEVWLPGRTFVTPEI
                     LDAAPRLRLVQYFGTGYDRANVPELERRGLRLARVPATNARSVAELNLLLMLALGRRF
                     QDIPAALAQGLQAHPMGRELGGATVVIVGLGACGGELARLCRGLSMRVVAAVRRPDSV
                     TDPAVDAVVSSTGAGFHAALARADFVCLHARLTDETRGMFGAAELAAMKRGAYLVNTA
                     RAELVDRAALEQALDAGRIGGCALDVFWTEPVPADDPLVHHPRCIVTNHVAGFTEQST
                     RSSFELVARNLDALAADRPLLGQVL"
     gene            complement(7307..8035)
                     /locus_tag="VF516_13515"
     CDS             complement(7307..8035)
                     /locus_tag="VF516_13515"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8108745.1"
                     /translation="MTDRIVLITGGSRGLGGALVSTFLQAGDTVATFSRSSTALVEKC
                     QAEYPGRYVWESIDGIDFDKAAGFVRTTYRRFGRLDVVVNNLGVMKDGLLPLMQNEHI
                     HQVIALNVECTARIAREAARYMTKQGSGVIINVSSVTGLRGFRGVAVYGATKAAIDGL
                     TRGLARELGSRGIRVNSVAPGHLETETGAHLNPQQLEQIARRTPLQRLPEMGDVTPVV
                     EFLASPAAGFITGQTLVIDGGLTC"
     gene            complement(8064..8819)
                     /locus_tag="VF516_13520"
     CDS             complement(8064..8819)
                     /locus_tag="VF516_13520"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8108746.1"
                     /translation="MTTPVLQIEHRGAVSEIRLCREEAHNALDTALLQALDDAIADVT
                     GRPATRALIVSGQRVFCSGADLEHLATQSPEQVRAFCALGTGVLDRLASAPILTIAAV
                     GGAALGGGFELVLACDMAVATPGALFGLPEIQLGAIPSFGGVRRLCRRLPPSTARELV
                     YSGRTLTAARAKELGLVLDVVKPTDLVEHCATLASRIARNAPLALAAAKRLFVADELG
                     DGSVERELAEAQQLVSTADWHEGLTARRNKRRP"
     gene            complement(8842..9993)
                     /locus_tag="VF516_13525"
     CDS             complement(8842..9993)
                     /locus_tag="VF516_13525"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8108747.1"
                     /translation="MSNWQPIPSTLLAQMPRTYAMADLVAEREAMRARGIDLLDVSIG
                     ELHLAPDAQTREALARAAADVAFNRYPELRGSRELRAAFADFYRRRYGVELDPDREVL
                     ALLGTKEGIVNLARAFADREHPVYCGRVGYPVYRAAALQASAELVALGGEWEDGYRPA
                     FTGPPRRGGVAFVCSPANPTGAVLDRADIERFAAQCRERDVVLCFDAAYAELSGTGAP
                     LLAIPTVGRRGVVELHSLSKSLCLAGWRVGFAVGDPDLIEGLARVKSFCDSGVPTPQQ
                     HALRHLLPMCDGILDRTRRQIADLQEVLRAQLAALGCEIFETRAGLFCWLKPGRPGTE
                     FARACLNEGVVLVPGAVFGPGGVDCVRISATLSADRMAELAERLRRVLR"
     gene            complement(9990..10958)
                     /locus_tag="VF516_13530"
     CDS             complement(9990..10958)
                     /locus_tag="VF516_13530"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108748.1"
                     /translation="MADDRSTTFIVGLGVVLPPQHRVPAGGDLAYRGWNHYRRAAEQD
                     HPSTMGARAVADALARASLQPADLSLVIYAGVSRDYPPSWSAAVAIMDEIGVGTGCVG
                     MDIINGCLGALLGLEIARRWLRGGEGQHVAVVCAERWAGSIARDDAAQKRLWGNSDGA
                     AAAIVSASPAGAALEVASVSLLNVPEYVDHVLVKYGGTRHPVAPPHEDPQRRILSGRP
                     YEQLTDHYRASYESVATRAIALANHRPDTLVCNQISPNVVDLIADALAYGPAQVIRTG
                     TENGHMGPADVLVGLRAAVDLGCSTVLAAASSPYSFGAAVLRRSAG"
     gene            complement(10951..11640)
                     /locus_tag="VF516_13535"
     CDS             complement(10951..11640)
                     /locus_tag="VF516_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108749.1"
                     /translation="MLSGRVETVLRWMLVGGLVCGAIGLLSQVACAIALISALYLLGM
                     RHGLRTHHTTIAIKLWLLAFAFTPSTDVWSLDAVIARQLGTEIAPAPEAYGWCLQLCR
                     TALAVVIFATGVAKVRNMSRNAGFCHRGNLSDLLRLHDYPLFFVKPLFSLSRFIRRSP
                     WLERAFAVGVVVAELGFPITLFWPVAGWFFVPAILAMIIGFRLFIGARFDLLAVGVVV
                     FFLPWERLLHG"
     gene            complement(11852..12370)
                     /locus_tag="VF516_13540"
     CDS             complement(11852..12370)
                     /locus_tag="VF516_13540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108750.1"
                     /translation="MLSLVALLVLAGHAVAIVRFRSYWPFDHYPMYSFPISRMRYPIV
                     RGGHLTMFSLVEVNDDGTTTDLLSGADIYPEAFHPLDRIEVVVTLVRAGMLEGLEREL
                     PASQHIDDRGAGSMAQLQKALCDLLEFARTNRPSLSTLRLVALEWSDFRNPQSDFKHP
                     DRSRVIAEVSHS"
     gene            complement(12390..13202)
                     /locus_tag="VF516_13545"
     CDS             complement(12390..13202)
                     /locus_tag="VF516_13545"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8108751.1"
                     /translation="MPTVTVNGISAQVSETGEGEPVVLLHSGAGEARDWRRFTAALPA
                     GYRCAALDFYGCGGTPAWPGPAALTMDDQAHLVATLARSIGAPVHLCGHSYGGAVALR
                     LAVTQPELVRTLSLIEPQCYPLLREISDPLFEVSESLWHSFCAALERGEPEHGWRQFI
                     DYYSGDGFFDRLRPEMRASFVAVSPIERWAVLFSNPTTIDDARRVQVPTLVLCGENTT
                     PPERRMCEIIASAVPRATLGVLPGAGHMSPITHPKEVAGTLARYISTHLGAS"
     gene            complement(13242..14006)
                     /locus_tag="VF516_13550"
     CDS             complement(13242..14006)
                     /locus_tag="VF516_13550"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8108752.1"
                     /translation="MTQSVEDFTRLIRSRLQDEVRREVEEGHAFFRTWYAGKLTRDQV
                     GAFYQTIYFEAVHAGRLLGRLLSVAPNRDAFNVVAQNMGEELGAGQIENSHPELILRL
                     AGRYGRPIPRVLEEGPVAPLATLWPELGIQYADRSFAAGLASFTYIEAGVPSRHAQQR
                     KALVEHYGAEPALLAFHDQHLSMDAAEAAGGKNITDASVPGYGGDDVHVERQVGHIAR
                     FARSADDQNLVLQAISAICDAKIKYYCQCNEMIAEA"
     gene            14338..>17271
                     /locus_tag="VF516_13555"
     CDS             14338..>17271
                     /locus_tag="VF516_13555"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX8108753.1"
                     /translation="MAAGPPKGDLPEPATVNTFAKTFTKIVQRLTPPLGCDRLTIADG
                     LPNSNVTAIAQDARGFMWFGTQDGLARYDGTRMTVYRPNDQDPHSISSGYITSLALDS
                     SGKLWIATSEHGVNLYDPETDRFERIGPGKGGLTSEGVTAIVRDAKNRIWFAMGDGGL
                     NRYDTATRTITAYLAKPLDVPITAIDADREGNLWLGTASETVLRWNPDNNGATVSFRP
                     NAESARATPITALRARSSGQVWIGTDGDGLFRLDPTTKQVARYRSDPNDWGTISDDHV
                     SVLREDRHHTLWIGTANGLDQMADTGRVVRYQHDPNDPDNPMNLAFASVESLFQDAGG
                     VLWTGGFTVGVCKFDEFRMKFGHYRTNSYAKSFFQDPDGSLWVGTLNGLYRYDWSAGR
                     VTIYHSLGRPVGAVQEPVALESVWLVALHKDRRGTLWISVGRKGLVGFDPTTDTYRQY
                     LPNTEDPDSLPADTIYDIAEDEHGDLWLASWGGGLVRFDPRSETFTSFTTNDSSGISS
                     NHLYAVYPDPKEKNLLWVGTAKGGLDRFDLAQHTARAYHHDDKDPSSLSGDDIVSVYR
                     EPSGTLWVGTYSSGFDRLDPQTGKAERFTTSNSRLTNDTVYGILSDDTGKLWLSTNGG
                     GLLQFDPQTKQITAYDSSEGVQDNEFSQGAFMRNKSGELFFGGPRGFNAFFPRDIKRD
                     PYVPPVVVTGFKRFNQDLALTRPVWTLPSITLSYSDSFEVQFAALAFAAPKRNRYAYK
                     LEGFDSKFIETDRPYATYTKLAGGDYTLRIRAGNQDGDWNEQGIALKLTVTPPWWRTW
                     PAYIAYVLLMVGAGVTLIQVQKARVMRAEREGRLAVVERDLALTGAVQTGFLPEQNEI
                     STPGFQLVGVYRPADSCGGDWWWHEPLPGGRHLVMVGDVTGHGPGPAMVTAAVATALR
                     VLVGSGVNDVEHTLTLLNREVLRVAKGKYHMTMAAMELDEASGHWRLLSAGAPPLLTL
                     GERGEH"
BASE COUNT         2850 a         5935 c         5749 g         2737 t
ORIGIN      
        1 gagatgggct cgctgttcgc gacccagcag aacatccgca tctgcgccag ccagccgaac
       61 gtgaccttct cggggacgtg ccggttcccc aacgcggtga cggtcgcggc cgccaacggt
      121 cacttccaca agcgcggcaa gtccttcacg atcttctcgt ggaacggcac ctcgctcgac
      181 cacccggccg acacctcgat gttctatgag agcgacacct ggaacgatcc accgatggcc
      241 acgaacctga gcgtcgccga gccggcgagc tcgggcatct ggtgggactg tgcgtaccag
      301 tggcagccgc cgtcccagtt cacgtgcgac gacgtcaatg ccaaggatcc gctgcaccag
      361 aacgactgct gttacacctt cggcggaatc accgacgtcg gtgagcactg caacgtgttc
      421 ctgtactact atcccaaggt cgagtcggac gtattctgtc tgtgatcgga gcggtgatcg
      481 gaccgccggg gcgtcgccgg ggtcaatgga ttccggcagc cgcggcgagc ttgctgctcc
      541 tgccggccgg gtgcggcggc aagcacgagg ccggaatcga cgcgggaccg aagtgtccga
      601 tcggcgatct cgaggcgccc gccgagctcg agatcgttca ccaggacgcc accagcacgg
      661 tcctccagac ccagtcgatg gctcagatcc cgctgttgcc gccgccccag ggtgggtgga
      721 tcgtcctgct cggcgcacgc gcgcggaaca tcgatggctg ccaggtcacg ctgacgacgg
      781 cgctcgtgga ccgctgcgat cagcagatca tccagatcga caagcgcccg acccgtctgg
      841 agccgggcgc cgatggctgg gggacctcga ccgtgacgac gttcggcaac ctgccggtct
      901 gtccggagct caccgcgaag cgggacctcc atgacgtgcc ctacgacgtc acggtggtgg
      961 tcgaggacag cggcggccag aaggcctccg ccacgctccc cgtcgtgcct acctgcccga
     1021 ccggcgacgc gctgtgcctg tgtcagtgcg atcgcgacta tgtcatcgga agttcctgca
     1081 acccaccgcc gaccggcggg cacggaacct gtccgtccac agggccgtaa attcacgcga
     1141 cccgacctgg atctacccgt caagaattct ctgtggtcct gctttcgttc cttaccgcgg
     1201 ctcacctcgg tccggcgccg acgatgtagt gggccgtctc actcagcgac catcgagtcg
     1261 catgcgacac ttggcgagcc gcacgccggc ttgatactgt gtgcgcacag gcgggcgggc
     1321 accacgcggg atgaccacgc tggatcgaga agtcatggat taggagtcat gatgttggaa
     1381 gtccacgtcc gcacgctgat tctcgaaagc ctccaagagg tcatccagga aaagccgatc
     1441 gacaagcagt tcgaggtctc cgatcgcacc aagctcctgg cgaagggctc ggagatggat
     1501 tccctcgact atctcaactt ctcgacctgt ctcgaggaac gtctgttccg ggcgatcggt
     1561 cgggaggtgg acctgtcgtc actggtcgtc gatcatgctg atgcctttca ggatgtcaat
     1621 cgactggccg ggttcttggc ccctcgactg gcacagtgac agtgaccaaa ccagattggg
     1681 tcgagaccag gcgacggctg gagcctggcg agctcgaacc attgtacgcg ccattcctca
     1741 tgtccggcgg gatgggacag gagcggatcg cgttcgactg ggtgcaggtc gcaggaaaga
     1801 cggtgacggc cagctgtcac ctctccgatt acttcaaatc tccctgggat ttcgagttcc
     1861 acctcaacgc cgtgaccggc atggcgatga tctcgcagct ggcgatgatt cacggcatgg
     1921 tcctttgcgg cgtcgatcgc aaggacttcg agatcctgat gatggactac cagcagacga
     1981 tgacgaacct catccgcgac cgcgagggca tccggctctc catgacggtc gtgcagcgaa
     2041 ccgtcgtacc tgcaggctgg cgcgggcagc acccgcgcac cttcttcaag tggacgttca
     2101 ccgtgaacga cgggacctgg gtgggctcga tcgccgcggc gttcccgttt ggcccccatg
     2161 gcgaggggct ggagctcgtg cgccggcagg acggcaagac ggaaccatga tggctacgct
     2221 ctccgcatcg caggcgctgc tggacagcct cgaccgaagg cgggatcaca ccgcgctcta
     2281 tcaagcaggg cgcgaccaca ccatcgccga gctgctcgac cgtgccggag agtgggtcgc
     2341 ctacctgcga gctgcgggca ttggcccggg atcggtgtgc gccgccaccg gcgagttctc
     2401 gttcaacacc agcgcctttc tgatcgcgac cttgatgctg cgctgcacgg tggtccccct
     2461 cgcggcggat tcgggcgaga actcacagct cctgggcctc gcagccgccg atatcgtggt
     2521 ggatctcgat cgcgatgccc tccatcacca ggtggtcacg gtcgcgaagc acgacagccg
     2581 gctcctgcgg gactttctga acaccgggca gccgggcatc atcacgttca cgtccggatc
     2641 cgccggcgag cccaagggcg tcctccacga cttcgatcga gccttgaaga acttcgggcg
     2701 cttcgatcgc aaggggcacc gctcgctgat ctttctccgg ttcaaccacc tcggcgggat
     2761 caacacgctg ctctcggtgc tcgcgtcgag cggtggtgtc gcgatctttc ccgagcagcg
     2821 caccgccgag gagatcgccg cgctggtcgc gcgagcccgc gtggagctgt tgcccgtgac
     2881 gcccacgttc ctcgcgatgc tgctggcgtc gcgcggccac gagcgccacg acatgagctc
     2941 gctcaggctc atcagctacg gcgccgagcc catgccccag accttgctgg accgcatgaa
     3001 ccagctgttt ccgcaggtcc ggttccacca gacctacggt acgaccgagc tgtgcctgct
     3061 gcggacccag tctcgctcgt cggactcgct gtgggtgaag ctcgggggtg acgggttcga
     3121 gatccgcgtg atcgacggac tgctctacgt ccgatccgag ttcgccatgc tcggctatct
     3181 caacgctccc aatccgatcg gaccggacgg ctggctggca acgggcgatc gcgtggagca
     3241 ggatggcgag tggatccggt tcctcggccg cgactcggac atcatcaacg tcggcggcga
     3301 gaaggtcacc ccgctggaga tcgagtcggt gctgatcggc gccgagaaca tccgcgacgt
     3361 tcgcgtgtac ggcgagaagg acgcggtgtt cggcgcgcgg gtcgttgccg acgtccagct
     3421 gctgaccgac gaggacccga ccgagttgcg gcgacggctc cgccggatct gcctcgagaa
     3481 gctggcgcgc tacaaggtcc cggtcaagtt caacatcgtc acgaggtcct tccattcggg
     3541 acgattcaag aaggtgagag catccgggcc gccgaggaca tcgatgctga agaccgaagc
     3601 ctgggttctc cacgagggtc ccctccccgt caagggcgac cgaccgcagc ttccgcgcgg
     3661 ctcccagggc atctttcccg acggcgcgtt cggtctcgag accctcgagc tccgcgagcc
     3721 gaccggcgac gagtgcttga tcgagccgct ctacggctgc tgggagggga acttctccca
     3781 cgccatcgcg cgtgtcccgg tcgacatctg ccggcagcgc ggggagagca aggtggtgct
     3841 gggcaacagc ggcgtggcac gcgtgctccg gcccggtccc gacgccaagg ggctccgcga
     3901 gggcgacctg tgcctcctgt tcggcaacgg ggtggccgac cgctatggct acatgcagct
     3961 ggcccacgca ttcgacgcgc ccaacacgat ggggctcttg gccaagcgcg cgaacgtgcc
     4021 ggaccgcaac ctcatgccga tcccgcgcga ctcgcccttc acgctgccgc aatgggcagc
     4081 gttctcgctg cggtacatca cggcgtgggc gaaccatcgc gtcgcctacg gtgcctggcg
     4141 cctgcaggtc cccgagcagc tgtttcccga ggcgtttccg gtctgggcct ggggcggcgg
     4201 ggtggccttc gcgcagctga ccctggccag gatcaaggac tccgcgcggt gcgtcctcat
     4261 cgcatcatcg ccggcccggc gcgagctcgc cgcacgggcc ggcctggaaa cgctcgaccg
     4321 ctccctttac cccgagctct ccttcgatgc atcgcgttac gacgcggatc ccgagtacgc
     4381 cgcgcgctat cgcgattgcg agaagcgctt cctccaggag gtgcgcgcgc gaaccgacgg
     4441 cgagggcgtg gcgatcttca tcgacaacat cggcgcgccg gtgtttcgcg tgacgctcaa
     4501 ggcgctggcc cgcgagggtg tcgtggccac ctgcggctgg aagaagggga tgcgcctgga
     4561 gatccttcgc gcgatggaat gcatcgcgcg ccacaccttc gttcatacgc attatgccag
     4621 ccgtcaggaa ggcgaggacg cggttcgctt cgcctgcgag cgcaactgga tggttccccg
     4681 ggacgcgctc ggcaagatct gggagtggaa cgaggtcgcg aagctggcag agggtcacat
     4741 gacaggtgcc atcgacagct atttcccgat cttccggatc aaccccgagt gatcggcgtg
     4801 atcacgcggc gcacgcacgg tcccgtcgtc gagctgtgcc tcgaccgcag gccggccaac
     4861 gcgctcggcc gcgccgcctg cgagcagtgg acggcagccc tgcgcgacgc agtcaccgcg
     4921 ggtgccgatg ccatcgtgct gagtggcccg agcggtgtgt tctccagcgg cgcggatctc
     4981 cccgagctgg cccgcgccga ccgcagcggc gtcgctgcgt tctgccgcgc gctgggcggg
     5041 ttggtcaccg aggtggcgca ctgcccggtc ccgatcgcag cggcgctgac cggccattgc
     5101 ctgggggccg cgtccggcgt ggcgctgctg tgcgatgccc gcttcatggc gaccggccat
     5161 catcggttcc ggatcaatta tgtcgccatg ggcctggtcc cgccgctgta cgtccgccgc
     5221 gccgtggagc ggctcgtcgg ccccggccag gctgcgcggc ttctggtcgg cgcgctcgcc
     5281 gtcgcgcccg acgaggctcg agcactgggc ctcgtggagc ggaccgccgc gcccggcgag
     5341 gtggtcggcg cggccctcga ttggtgcaga cagcagctgg cgctaccacg caccgccatg
     5401 ctcgagaccc gcaagcgcct gcgcgaggag ctcgtggcat gcgtcacgcc cgagaacctg
     5461 gggatcgacg agatgacgga tcgctggttc ggcgccgaag ttcaagttcg tttggccaag
     5521 ctggtcaccg cccaggacgg gcgcgtgatc gccaacggcg aatgaggaag gagatctcgc
     5581 atgtcacgaa tagctgcgct tgtctatggt ggaatcgcct acctgggctt cctggcggtg
     5641 accctgtacg ccatcgggtt cgttggcaat ttccatgtcc ggatcgccga cggcatctac
     5701 ttcgtcccgc aggcggtcga tctcggcagg cctgcggtgc cgaccctgga ggccctgctg
     5761 atcgacggtg cgctgctgct cctgttcgcg gtccagcaca gcggcatggc tcgccagagc
     5821 gcgaagtcgc ggctcacgcg cttcatctca ccggccatcg agcgcacgac gtacgtgatg
     5881 gcgaccaacg tggtcctggc cctgctgttc tggcagtggc gcgcgatcgg cggcacgcta
     5941 tgggcattcg gtgacggaac cagcgccgcc gtcgtgcttc gcgtcctgaa cctcggcggc
     6001 tgggtcctgg cattcgtcgc cacgtttctc atcaatcacc tcgagctgtt cggcttgcag
     6061 caggtgtacg cgcatgcgcg cggcgccgag ctgtccgggc cgacgttccg gcagccgtat
     6121 ctctaccgct ggatccgaca tccgctgtat ctcggcttct tgatcgcgtt ctgggccacg
     6181 ccggtgatga cctggggaca cgtgttgttc tccgtcgccg ccaccggcta catcctggtg
     6241 ggggtccagc tcgaggagcg agacctgatc cgggtgttcg gcgaccgcta ccgcgagtat
     6301 cgccagcgcg ttcccatgct gcttcctcgg cccggatccc gccagaagga aaccgtgtag
     6361 ccggtgcgcg tgctgggcac acatcacgct cacctgatga gcgtgctgcg ggacctggtc
     6421 gccgatcgac acgtgatcga tgtcctcgac cggtcgctgc ccatcgcgga gcagatcgga
     6481 caggccgagg tctggttgcc ggggcggacg ttcgtgacgc ccgagatcct cgacgctgcg
     6541 ccgcgccttc gactcgtcca gtacttcgga acgggatacg accgcgccaa cgtgcccgag
     6601 ctcgagcggc gcgggctccg gctggctcgg gttcccgcca cgaacgcgcg ctcggtggcg
     6661 gagctcaacc tcctcctgat gctggcgctc ggccgccgct tccaggacat cccggcagca
     6721 ctcgcgcagg gactgcaggc ccacccgatg ggacgcgagc tcggcggcgc cacggtcgtg
     6781 atcgtgggac tgggggcctg cggcggggag ctggcgcggc tgtgccgcgg cctgtcgatg
     6841 cgcgtcgtgg cggcggtgcg gcggcccgac tccgtgacgg atccggcggt cgacgcggtc
     6901 gtgagctcga cgggggcagg ctttcacgcc gcgctcgctc gcgcggattt cgtctgtctg
     6961 cacgcccggc tcaccgacga gacccgtggg atgttcgggg ccgcggagct cgcggcgatg
     7021 aagcgcggtg cgtacctcgt caacaccgcg cgggcagagc tcgtcgaccg ggccgcgctc
     7081 gagcaggcgc tcgacgccgg gcgcatcggt gggtgcgccc tggacgtgtt ctggaccgag
     7141 cccgttccgg ccgacgatcc gctcgttcac caccctcgtt gcatcgtgac caaccacgtc
     7201 gccgggttca ccgagcagag cacgcggtcg agcttcgagc tcgtcgcgcg caacctcgac
     7261 gcgctggcgg cggaccggcc tctcctcggt caggtcctgt gaacggctaa cacgtcagtc
     7321 caccgtcgat gacgagcgtc tgtccggtga tgaaaccggc cgcgggtgac gcgaggaact
     7381 cgaccaccgg cgtcacatcg cccatctcgg gcagccgctg cagcggcgta cgccgcgcga
     7441 tctgctcgag ctgctgcggg ttcaggtggg ctccggtctc ggtctcgagg tgaccgggcg
     7501 ccaccgagtt gacccggatc ccgcggctgc ccagctcgcg cgccaggcct cgcgtcagcc
     7561 cgtcgatcgc ggccttggtg gctccgtaca ccgccacgcc gcggaagccg cgcaggccgg
     7621 tcaccgacga gacgttgatg atcacgcccg aaccctgctt ggtcatgtag cgcgccgctt
     7681 cgcgcgcgat ccgcgcggtg cactccacgt tgagcgcgat gacctggtgg atgtgctcgt
     7741 tctgcatcag cgggagcaat ccgtccttca tcacgccgag gttgttgacg acgacatcga
     7801 gccggccgaa ccggcgatac gtggtccgga cgaagccagc cgccttgtcg aagtcgatgc
     7861 cgtcgatcga ctcccacacg taacgaccgg ggtactcggc ctggcacttc tcgaccagcg
     7921 ccgtcgagga ccggctgaac gtggccacgg tgtcgcccgc ctggaggaac gtcgagacca
     7981 gtgcgccacc caggcctcga ctgccacccg tgatgagaac aatcctgtcg gtcatggcat
     8041 gactcctttg tacttccgct cgatcacggg cggcgcttgt tccgtcgcgc ggtcagcccc
     8101 tcgtgccagt ccgccgtgga caccagctgc tgtgcctccg cgagctcgcg ctcgacactg
     8161 ccatcgccga gctcgtccgc gacgaacagc cgcttggcgg cagccagcgc cagcggcgcg
     8221 ttgcgcgcga tccgcgacgc gagcgtcgcg cagtgttcga cgaggtccgt cggtttcacc
     8281 acgtcgagga ccagccccag ctccttggcc ctggcggcgg tgagcgttcg cccgctgtag
     8341 accagctcgc gcgcggtgga cggcggcagc cggcgacaca gccggcgcac cccaccgaag
     8401 ctggggatcg caccgagctg gatctccggc aggccgaaca gcgcaccggg ggtcgccacc
     8461 gccatgtcac aggccaggac cagctcgaag ccgcccccca gcgcagcacc accgaccgcc
     8521 gcgatcgtga ggatcggcgc gctcgccagc cgatcgagca cgccggtgcc gagggcgcag
     8581 aacgcgcgca cctgctccgg gctctgcgtc gccagatgtt ccagatccgc cccgctgcag
     8641 aacacccgct ggccgctcac gatcagcgcc cgggtcgccg ggcgcccggt cacgtctgcg
     8701 atggcgtcgt cgagcgcctg caacagcgcc gtatcgagtg cgttatgagc ttcctcgcgg
     8761 cacagccgga tctcggacac ggccccgcga tgctcgatct ggaggacggg tgtggtcatg
     8821 gctgctggtt tcctcctgag atcatcgtag gacgcgccgc agccgctcgg ccagctcggc
     8881 catgcgatcc gcggacaagg tcgcgctgat ccgcacacag tccacgccac cgggaccgaa
     8941 caccgcaccc gggacgagca cgacaccttc gttcaggcac gcacgcgcga actcggtccc
     9001 gggccgcccg ggcttgagcc agcagaacag gcccgcacga gtctcgaaga tctcgcagcc
     9061 cagagccgcg agctgtgcgc gcaacacctc ctgcaggtcg gcgatctgcc ggcgcgtgcg
     9121 atcgaggatg ccatcgcaca tcggcaggag atggcgcagc gcgtgctgct gcggcgtcgg
     9181 cacacccgag tcgcagaacg acttgacccg ggccagaccc tcgatgaggt cggggtcgcc
     9241 gaccgcgaag cccactcgcc agcccgccag gcagagcgac ttggacagcg agtgcagctc
     9301 gacgacgccg cgccggccga cggtggggat cgcgaggagg ggggctccgg ttccggacag
     9361 ctcggcatag gcggcgtcga agcacagcac cacgtcgcgc tcgcggcact gcgccgcgaa
     9421 gcgctcgatg tctgcgcgat ccaggacggc gccggtcggg ttcgccggcg agcacacgaa
     9481 cgccacccca ccgcgtcgag gtgggccggt gaacgccggg cggtacccgt cctcccactc
     9541 gccgccgagg gcaaccagct cggcgctcgc ctggagcgct gcagcccggt agaccgggta
     9601 accgacccgg ccacaataga ccggatgttc gcgatctgcg aacgcccggg ccaggttgac
     9661 gatgccctcc ttggtgccga gcagggccag gacctcgcgg tcgggatcga gctcgacccc
     9721 gtatcgccgg cgatagaaat ccgcgaacgc agctcgcaac tcgcgactgc cacgcagctc
     9781 gggataccgg ttgaacgcca cgtcggcggc cgctcgggcc agcgcctcgc gggtctgcgc
     9841 gtccggcgcg agatggagct cgccgatcga tacgtcgagc aggtcgatgc cgcgcgcccg
     9901 cattgcctcg cgctcggcga cgagatctgc catcgcatag gtcctcggca tctgcgccag
     9961 cagcgtcgaa gggatcggtt gccagttgct catcctgcgc tcctccgaag tacggctgcg
    10021 ccaaaggaat acggactgct ggcggctgcc agcaccgtcg aacagcccag gtcgaccgcc
    10081 gctcggagcc cgaccagcac atcggccggg cccatgtgcc cgttctccgt gccggtccgg
    10141 atcacctgcg cagggccgta cgccagcgcg tccgcgatca ggtccaccac gttgggagag
    10201 atctgattgc acacgagggt gtcgggccgg tggtttgcca gggcgatcgc ccgcgtggcc
    10261 acgctctcgt aggaggcccg atagtggtcc gtcagttgct cgtaaggacg gccgctgagg
    10321 atccgccgct gcggatcctc gtgcggaggc gcgaccgggt gacgcgtgcc accgtacttc
    10381 acgagcacgt gatcgacgta ctccggcacg ttcaacagcg agacgctggc gacctccagc
    10441 gccgctccgg cgggcgaggc cgacacgatc gcggcggccg cgccgtccga gttgccccac
    10501 agccgcttct gggcggcgtc atcgcgcgcg atcgaaccgg cccagcgctc ggcacacacg
    10561 accgcgacgt gctggccctc tcccccgcgc aaccaccgcc tggcgatctc cagaccgagc
    10621 agggcgccca gacatccatt gatgatgtcc attcccacac accccgtccc gacaccgatc
    10681 tcgtccatga tcgcgaccgc cgcggaccac gacggcgggt aatcgcgcga gactcccgcg
    10741 tagatcacca gcgacagatc ggcgggttgg agcgacgccc gagccagcgc gtccgcgacg
    10801 gcgcgtgcgc ccatcgtgct cggatgatcc tgctccgccg cgcgtcgata gtggttccag
    10861 ccgcgatagg cgaggtcgcc gcccgcgggg acgcggtgct gcggggggag cacgaccccc
    10921 agcccgacga tgaaggtcgt ggatcgatcg tcagccatgg agcaggcgtt cccagggcaa
    10981 gaagaacacg acgacgccca cggccagcag atcgaaacga gcgccgatga acaggcgaaa
    11041 cccgatgatc atggcgagga tcgcaggcac gaagaaccag ccggcgaccg gccagaacag
    11101 cgtgatcgga aaccccagct ccgcgacgac cacgccgacc gcgaacgctc gctccagcca
    11161 cggagatcgc cggatgaatc gactcaagct gaacagcggc ttgacgaaga acagcgggta
    11221 gtcgtggagc cgcaacagat cggagaggtt cccgcgatgg cagaagcccg cgttcctgga
    11281 catgttgcga accttggcaa cgccggtcgc gaagatcacg accgcgaggg cggtcctgca
    11341 cagctgtaga caccagccgt atgcctccgg tgcgggggcg atctcggtgc cgagctgtct
    11401 ggcgatcacc gcgtccagcg accacacgtc ggtcgatggc gtgaacgcga acgccagcag
    11461 ccagagcttg atcgcgatcg tcgtgtggtg ggtcctgagg ccgtgccgca tgcccagcag
    11521 gtacagcgcg gagatcaagg cgatggcgca ggcgacctgc gagagcaggc cgatggcgcc
    11581 gcagaccagg cccccgacca gcatccagcg caggaccgtc tcgacgcgcc ccgacagcat
    11641 cggaacgcga accgctcgca gcaaccacgg tggctgccag tgcgccgcca ggtaggtccg
    11701 ggcgcgttgc caggccggaa agtcgtaggc cggatcccgg agcttgaaca gcgcgtagcc
    11761 caggatggcg gctctcgcca gggcgacctg tgccccggtc tgcggagcga accagtagtg
    11821 gtcccagctc cgctcgagct gtagcagtag ttcaggaatg gctcacttcc gcgatgaccc
    11881 tcgatcgatc agggtgtttg aagtccgatt ggggattccg gaagtcagac cactccaggg
    11941 cgaccagccg gagcgtgctc agcgatggtc ggttcgtccg cgcgaattcg aggagatcgc
    12001 agagcgcttt ctggagttgc gccatcgagc ccgcgccgcg gtcgtcgatg tgctggctcg
    12061 cagggagctc ccgttcgagc ccctccagca tgccggcgcg cacgagcgtg acgacgacct
    12121 cgatgcgatc gagggggtgg aacgcttcgg gatagatgtc cgcgccgctg agcagatccg
    12181 tcgtcgtacc gtcgtcgttc acctccacca gcgagaacat cgtcaaatgg ccgcctcgca
    12241 cgatcggata tcgcatccgg gaaatcggaa acgagtacat ggggtagtga tcgaagggcc
    12301 agtacgagcg aaatcgcacg atcgccaccg cgtggccggc gagcacgagc agggcgacga
    12361 gcgagagcac gaggtgcatg gcgcggctgt cagctggctc ccaggtgggt cgagatatac
    12421 ctggcgagcg tgcccgcgac ctccttcggg tgagtgatcg gggacatgtg cccggccccg
    12481 ggaagcaccc cgagcgtggc gcgcggcacc gcgctggcga tgatctcgca catgcgacgc
    12541 tccggcggcg tcgtgttctc accacacagc accagcgtcg gcacctgcac tcgccgcgcg
    12601 tcgtcgatcg tcgtggggtt cgagaacagc acggcccagc gctcgatcgg cgacacggcc
    12661 acgaagctcg ctcgcatctc cgggcgcagc cggtcaaaga acccgtcacc gctgtagtag
    12721 tcgatgaact ggcgccaccc gtgctctggc tcgccccgct cgagggccgc gcagaagctg
    12781 tgccacagcg actcggacac ctcgaacagg ggatcactga tctcgcgcag gagcggatag
    12841 cattgcggct cgatcagcga cagtgtccgc accagctcgg gctgcgtgac cgccaggcga
    12901 agtgcgaccg cccctccgta cgaatgcccg cacaggtgca cgggcgcgcc gatgctgcga
    12961 gcgagcgtcg ccacgagatg ggcctggtcg tccatcgtga gcgcggcggg gccgggccac
    13021 gccggcgtac cgccgcagcc atagaagtcg agtgctgcgc accggtatcc ggctggaagc
    13081 gcggcggtga aacggcgcca gtcgcgcgcc tcaccggcgc cgctgtgcag gagcacgacg
    13141 ggctctccct caccggtttc ggaaacctgc gccgagatgc cgttgactgt cacggtgggc
    13201 attcgcgtct cctgggcgtc tcttcatgca gctgctgccc gtcacgcctc cgcgatcatc
    13261 tcgttgcact ggcagtagta cttgatcttc gcgtcgcaga tcgcggagat ggcctgcagc
    13321 accaggttct ggtcgtccgc gctgcgtgcg aacctggcaa tgtgcccgac ctgccgttcc
    13381 acgtgcacat catccccgcc gtagcccggc accgacgcgt ccgtgatgtt cttgccaccg
    13441 gctgcttccg cggcgtccat cgacagatgc tggtcgtgga atgccagcag cgcgggctcg
    13501 gctccgtagt gctcgaccag cgccttgcgc tgctgggcat ggcgcgacgg aacgccggcc
    13561 tcgatgtagg tgaacgacgc gagtccggcg gcgaagctgc ggtcggcgta ctggatgccg
    13621 agctccggcc acagcgtggc gagcggcgcc accggcccct cttcgagcac gcgcgggatg
    13681 ggacgaccgt agcggccggc gagccggagg atcagctccg ggtgactgtt ctcgatctgg
    13741 ccagctccca gctcctcacc catgttctgg gcgaccacgt tgaaggcgtc gcgattgggc
    13801 gccaccgaga gcagtcgccc gaggagcctt cccgcgtgga ccgcctcgaa gtagatcgtc
    13861 tgatagaacg ctccgacctg atcgcgagtg agctttcctg cgtaccacgt gcggaagaac
    13921 gcgtggcctt cctcgacctc tcggcgcact tcgtcctgca ggcgcgaacg gatgagacgt
    13981 gtgaagtcct cgacggattg ggtcatctct acttctcctg ctcgtggatc tcagcttcgc
    14041 ccgagcaagc gactcccaac cagactcgcg cctacccctg cggatcgctc tcgatcagcg
    14101 ctgtcgtcgg accaccggca gctcggggga atcaccacgc gccggacgct aacacatcgc
    14161 gccacaacat acgacagccg gcccgtagga cctcgtgaca gcccggcgta ggcgtgtgta
    14221 ggaggccggc gacggtctac gtaccgcgct ccggatggtg tatgttgcaa gccaacaacc
    14281 atggtgcaga agctaaccgt cgcgtgggtg gtggcactct tggcggcacg gtcggcgatg
    14341 gcggcaggtc ctccgaaagg agacctcccc gaacccgcca cggtcaacac ttttgccaag
    14401 actttcacga agatcgtgca gcggctcacg ccgcccctgg gatgcgatcg cctgacgatc
    14461 gccgacggtc tgccgaactc caatgtgacg gcgatcgcac aggacgcgcg gggcttcatg
    14521 tggttcggca cgcaagacgg gctcgcccgc tacgacggga cccggatgac ggtgtaccgg
    14581 ccgaacgacc aggatcccca ttcgatctcg tccggataca tcacgtcgct cgcgctcgac
    14641 agcagcggca agctctggat cgcaacctcc gagcacggcg tcaacctcta cgatcccgaa
    14701 accgatcggt tcgagcggat cggccccggc aagggtgggc tgacctccga aggtgtcacc
    14761 gcgatcgtgc gcgacgcgaa gaaccggatc tggttcgcga tgggggatgg cgggttgaac
    14821 cgctacgaca cggccaccag gacgatcacc gcgtatctcg cgaaaccgct ggacgtgccg
    14881 atcacggcga tcgacgccga ccgcgaaggc aacctgtggc tcggcaccgc cagcgagacg
    14941 gtgttgcggt ggaaccccga caacaacggg gcgaccgtga gcttccggcc gaacgcggaa
    15001 tccgcgcgcg ccacgccgat caccgcgctc cgcgcgaggt ccagcggcca ggtgtggatc
    15061 gggaccgacg gcgatgggct gttccggctg gatccgacga cgaagcaggt cgcccggtac
    15121 cgctccgacc cgaacgactg gggcacgatc agcgacgacc acgtctccgt cctgcgcgag
    15181 gatcggcacc acaccttgtg gatcggcacc gcgaacgggc tcgaccagat ggcggacacc
    15241 gggcgggtgg ttcgctacca gcacgacccg aacgaccccg acaacccgat gaacctcgcg
    15301 ttcgcctcgg tggagtcgct gttccaggac gccggcggcg tcctgtggac cggcggcttc
    15361 acggtcggtg tgtgcaagtt cgacgagttc cgcatgaagt tcgggcatta ccgaacgaac
    15421 tcgtacgcga agtcgttctt ccaggatccc gacggatcgc tgtgggtcgg gacactgaac
    15481 gggctctacc ggtacgactg gtcggccggg cgggtcacga tctatcacag cctggggcgc
    15541 ccggtcggag cggtgcagga gccggtcgcg ctcgaatcgg tgtggctcgt cgcgttgcac
    15601 aaggatcgcc gcggaacgct gtggatctcc gtcggccgca aggggctcgt cgggttcgac
    15661 ccgaccacgg acacgtaccg gcaatacctg ccgaacaccg aggacccgga cagcctgccc
    15721 gccgacacga tctacgacat cgccgaggac gagcacgggg atctctggct ggcgtcctgg
    15781 ggcggaggcc tggtccggtt cgatccgcgc tccgagacct tcacctcctt cacgacgaac
    15841 gactccagcg gcatcagctc caaccacctg tacgcggtgt atcccgatcc caaggagaag
    15901 aacctcctct gggtcggcac ggcgaaggga ggcctcgatc gcttcgatct ggcacagcac
    15961 accgccaggg cgtaccacca cgacgacaag gacccgtcga gcctgagcgg cgacgacatc
    16021 gtcagcgtgt accgcgagcc gagcggcacc ttgtgggtgg gcacgtacag cagcggcttc
    16081 gaccgcctcg atccgcagac cggcaaggcc gagcgcttca cgaccagcaa ctctcgcctg
    16141 accaacgaca ccgtctacgg catcctgtcc gacgacacgg gcaagctctg gctcagcacc
    16201 aacggcggcg gcctgttgca attcgatccg cagaccaagc agatcaccgc gtacgactcc
    16261 tccgagggcg tccaggacaa cgagttcagc cagggagcgt tcatgcgcaa caagtccggc
    16321 gagctgttct tcggcggccc ccgcgggttc aatgcattct tcccacgcga catcaagcga
    16381 gatccctatg taccgccggt ggtggtgacc ggcttcaagc gattcaatca ggacctggcg
    16441 ctcacccgtc ccgtctggac cttgccatcg atcaccctgt cgtactcgga ctcgttcgag
    16501 gtgcagttcg cggcgctggc gttcgcggcg cccaagcgga accgctacgc gtacaagctc
    16561 gagggcttcg acagcaagtt catcgagacc gatcgtccct acgcgaccta caccaagctc
    16621 gcaggtggtg actacaccct gcgcatccgc gcgggcaacc aggacgggga ctggaacgag
    16681 cagggcatcg cgctgaagct gacggtcacc ccgccgtggt ggcgcacgtg gcccgcctac
    16741 atcgcctacg tgctcctgat ggtcggagcc ggcgtcaccc tgatccaggt ccagaaggcg
    16801 cgcgtgatgc gggccgaacg ggaaggccgg ctcgcggtcg tcgagcgcga tctggcgctg
    16861 accggtgccg tgcagaccgg ctttctcccc gagcagaacg agatcagcac gccggggttc
    16921 cagctggtcg gtgtgtatcg gcccgccgat tcgtgcggcg gcgactggtg gtggcacgag
    16981 cccctgccag gaggacgcca tctggtgatg gtgggcgacg tcaccggtca cggccccgga
    17041 ccggcgatgg tgaccgccgc cgtcgcgacc gcgctccgcg tgctcgtcgg cagcggcgtc
    17101 aacgacgtcg agcacacgct cacgctgctc aatcgcgagg tgctacgcgt cgcgaagggc
    17161 aagtaccaca tgacgatggc ggccatggag ctcgacgagg ccagcggaca ctggcgcctg
    17221 ctcagcgcgg gcgcgccgcc gctgttgacc ctcggcgagc gcggcgagca c
//
LOCUS       DASVOR010000685         4410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_64932,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000685 DASVOR010000000
VERSION     DASVOR010000685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64932"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF516_13560"
     CDS             <1..183
                     /locus_tag="VF516_13560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108754.1"
                     /translation="ERIRDHPERWPFYPHVPGGFRWCRFRRFPYAMIYELFPDVTHVL
                     AVAADRRMPGYWADRA"
     gene            complement(396..692)
                     /locus_tag="VF516_13565"
     CDS             complement(396..692)
                     /locus_tag="VF516_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108755.1"
                     /translation="MELSESMKKMWWRRRESKATMLTLHDAPGKEIREVPTAAAAPSV
                     AEHHGEAVDRSEVATAAPDPVIAELEAALAMRLAGADARALRRVLRRIEELLDE"
     gene            1224..2855
                     /locus_tag="VF516_13570"
     CDS             1224..2855
                     /locus_tag="VF516_13570"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8108756.1"
                     /translation="MSSLPSPQRAHVLDIPFAMRGIAAMSKARWDKEHGVFIYDGAEL
                     PPALAPFRAQPYSWEMHVQRELADAPPRKPSPPHASITLRAHQQEAARAIGAARVAGF
                     PGFLLADDVGLGKTITAWAAVLAMPAARRVLVVCPLAVIAHWRRTIEAMGDGGKDIVI
                     LNYDRLGKLFEVSDAARKKIRTKKGLARAGTAPELDVIILDESHRLKNPTAARSKLAA
                     KLVANAGFCLWLSATAGQNPLELSYLAPLLARVTGSSARDLKDFEQWCLDQQLGVTRG
                     NFGKWEWRGERSDCEKVRAMLFEPARAGQAIAGIRRQPEHIAGWPAINRILAPIELAA
                     EARALYEEAWTAFRREMELVPRGRDPKSALAASLRLRQKSSLIRAPGTIELARELLDN
                     GHQVAISVAFLETLTALREAFETGVGAVRCVAIHGGIPAAAREAERLRFQRGEARVVL
                     FTVEEGISLHQGEHDDAPRSEVIHDLRWSAIQMAQIEGRCHRDGRFAQVYWTYADDTV
                     EEKIARVVAGRIQSMKEMIGDDTETLREIENLLRG"
     gene            complement(2918..3481)
                     /gene="yjgA"
                     /locus_tag="VF516_13575"
     CDS             complement(2918..3481)
                     /gene="yjgA"
                     /locus_tag="VF516_13575"
                     /inference="COORDINATES: protein motif:HMM:NF016626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HEX8108757.1"
                     /translation="MADRDDRGESRRQIARRQQRKAGDRSARLANALMKLPVSALGKL
                     GLDEDLRASVDRARAVTAHVARRRAERTLAGDLRRIDLVALDARLAQVQTTGAAEPQQ
                     FHLAEQWRARMIEEGLAAAAEFPGGVTEPLPQLIASAQRERVTGKPPGAARALFRHIA
                     AVLKAQPVTPVASGEDAAADDADDEPG"
     gene            complement(3812..4309)
                     /locus_tag="VF516_13580"
     CDS             complement(3812..4309)
                     /locus_tag="VF516_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108758.1"
                     /translation="MTFIACLLVTRLVCPRRRSARRRIDDRDAVFSASNLAQLRADHL
                     ELRARRRGDARHLAAGRDLYQIRFVVTFVAVGVRFRPDMSVLRTGPCRAAGQGTNPWL
                     GSFAGLAATTATTATDDGGCNLDQLTGAGAAVELAAGASGAAMGHRWRPDRRALPDDE
                     AAFVE"
BASE COUNT          636 a         1638 c         1502 g          634 t
ORIGIN      
        1 gaacgcatcc gcgaccatcc cgagcgctgg ccgttctacc cgcacgtgcc gggcggcttt
       61 cgctggtgcc ggttccgccg gtttccgtac gcgatgatct acgagctgtt tccggacgtc
      121 acgcatgtgc tcgctgtcgc tgccgatcgg cgcatgcccg gctactgggc cgacagagcg
      181 tagctcgccc ccgcgctccg ggccgagctg gtcgccaccc caccgcgctg gcccaccctc
      241 gccgcgatcg ccgccgagcg ggtgacccgt gaccggcgcg ccgaggtcgc gctgctgcct
      301 gccgcgagca ggcgacgcag tgcagctcca gcgccacagc tgcgccgccg gtgctcggcg
      361 ccgaggtcct cgccatgcca cgccgagatc gctggctact cgtccagcag ctcctcgatc
      421 cggcgcagca cgcgccgcag cgccctcgcg tcggcgcccg ccagccgcat cgccagcgcg
      481 gcctccagct cggcgatcac cgggtccggt gcggctgtag cgacctcgct acgatccacc
      541 gcctctccat gatgctcggc aacactcggc gcagctgccg ccgtcggaac ctcgcggatt
      601 tccttgcctg gagcgtcgtg gagcgtcaac atcgtggcct tcgactcccg ccgcctccac
      661 cacattttct tcatgctttc gctgagttcc aagggctgtc acacggtccg ggtggcgtcg
      721 cgagatccgt agcttcgcga cctcggggca ggtcttcgcc caccggacgc cacgatcgta
      781 cccatcgaac gccgagcgac gcttcttggt gtgcgtgacg cggtgctcga ggacgtttgc
      841 atcggccccg tcctcgagcg cgagcgtgat gaacgtcgcg cgcatgtcat agtgcctccg
      901 gtgccgcatg ccgagcgccg gcaggtcgtc ccgggaccag cgcttgtagc tgtaatcggt
      961 gccgcggaac ggctcgcccg atcgtgtacg acgcttcgcc gcgtccctca ccaccagcgc
     1021 ggcctcgagc acggcggcgc ggcacggcac tgcccagccg acgtggttcg agctccttgg
     1081 ccgctcggcg cgctcgccgc cactccgggc cgggcgggtg agctccgggc ccgccactct
     1141 acgctccggc gggcgagccg ccacgtggtt gcccgcgtga ttcgagcgga ggtccggtca
     1201 gaggcccgtg atatctcgcc ggcgtgagct cgctcccatc gccgcaacgt gcgcatgtcc
     1261 tcgatatccc gttcgcgatg cgcggtatcg ccgccatgag caaggcacgc tgggacaagg
     1321 agcacggcgt attcatctat gacggcgccg agctcccgcc cgcgctcgcg ccgttccgcg
     1381 cgcagccgta ctcgtgggag atgcacgtgc agcgcgagct cgccgacgcg ccgccccgca
     1441 agccgtcacc gccgcatgcg tcgatcacgc tgcgagcgca ccagcaggag gccgcccgcg
     1501 cgatcggtgc ggcgcgcgtc gcgggctttc ccggtttcct gctcgccgac gacgtcggcc
     1561 tcggcaagac gatcaccgca tgggccgcgg tcctggcgat gccggcggcg cgccgggtgc
     1621 tcgtcgtgtg tccgctcgcg gtgatcgcgc actggcgccg cacgatcgag gcgatgggcg
     1681 acggcggcaa ggacatcgtg atcctcaact acgaccggct cggcaagctg ttcgaggtca
     1741 gcgacgccgc ccgcaagaag atccggacga agaaggggct tgcgcgcgcc ggcaccgccc
     1801 ccgagctcga cgtgatcatc ctcgacgaga gccaccggct gaagaacccg accgccgcgc
     1861 gctcgaagct cgcggccaag ctcgtggcga atgccggctt ctgcctgtgg ctctcggcca
     1921 cggcgggcca gaacccgctc gagctgtcgt atctcgcgcc cctgctcgcc cgggtgaccg
     1981 gcagcagcgc ccgcgacctc aaggacttcg agcaatggtg cctcgaccag cagctcggcg
     2041 tgacgcgcgg caacttcggc aagtgggaat ggcgcggcga gcgcagcgac tgcgagaagg
     2101 tccgcgccat gctgttcgag cccgcgcgcg ctggccaggc catcgcgggc atccgccggc
     2161 aaccggagca catcgccggc tggcccgcga tcaaccggat cctcgcgccg atcgagctcg
     2221 ccgccgaggc acgcgcgctc tacgaggagg cctggacagc gttccgccgc gagatggagc
     2281 tcgtgccgcg cgggcgcgat ccgaagtccg cgctcgcggc gtcgctccgg ctgcgccaga
     2341 agagctcgct gatccgcgcg cccggtacca tcgagctcgc gcgcgagctg ctcgacaacg
     2401 gccaccaggt cgcgatctcg gtcgcgttcc tcgagacgct caccgcgctg cgcgaggcgt
     2461 tcgagaccgg cgtcggtgcg gtccggtgcg tggcgatcca cggcggtatc ccggccgcgg
     2521 cccgcgaggc ggagcgcctg cgcttccagc gtggcgaggc gcgcgtggtc ctgttcaccg
     2581 tcgaggaggg catctcgctc caccagggcg agcacgacga cgcgccccgg tccgaggtga
     2641 tccacgacct gcgctggtcc gcgatccaga tggcgcagat cgagggccgc tgtcaccgcg
     2701 acggccggtt cgcgcaggtc tactggacct acgccgacga caccgtggag gagaagatcg
     2761 cgcgcgtggt cgccggtcgc atccagtcga tgaaggagat gatcggcgac gacaccgaga
     2821 cgcttcgcga gatcgagaac ctgctgcgcg gctgagcgcg gccgcgacgg cagcggatgg
     2881 atcgggccgc ggccagcccc aggtcagcgc cgacgcgtca cccgggttcg tcgtcggcat
     2941 catcggccgc cgcgtcctcg ccgctcgcga ccggggtcac cggctgggcc ttcagcaccg
     3001 cggcgatgtg tcggaacagc gcgcgcgccg cgcccggcgg cttcccggtc acccgctcgc
     3061 gctgcgccga cgcgatcagc tgcggcagcg gctccgtgac accgcccgga aactcggccg
     3121 cggctgcgag gccctcctcg atcatccggg cgcgccactg ctcggccaga tgaaactgct
     3181 gcggctccgc cgcgccggtc gtctgcacct gcgcgagcct ggcatcgagt gcgaccagat
     3241 cgatccggcg aagatcgccg gcgagcgtgc gctcggcgcg gcgacgcgcg acatgcgccg
     3301 tcaccgcgcg ggcgcgatcg acggacgcgc gcagatcctc gtcgagcccg agcttgccga
     3361 gcgccgacac cggcagcttc atgagcgcgt tcgcgagccg ggccgagcga tcgccggcct
     3421 tgcgctgctg acggcgtgcg atctggcgcc ggctctcgcc gcggtcgtca cgatctgcca
     3481 tcgcggcatc ttcgctcctt catccggcga ccaccagcga gcacgcgccg gatgaaacta
     3541 cggtcgctcg gcgcgccgat gcgtttcgag ctcctcgcgc ggaaacggca ccgtcttgcg
     3601 ctcgaacgcg agctggcgat ggaacgcgtt cagggcgacc acgaccgcgg catggtgccg
     3661 gacactcgcc gagacactcg ccggcgcgtg tgccagcgcg ccgccttcgg cacagcgccg
     3721 gagcagctcg tcgcaatcgg ccgcgccgcg ccgcgcggtg acagccagcc ggccactcgg
     3781 ccatcgtgta ccagcggtca tagccgacgt gctactcgac gaatgcggct tcgtcgtcgg
     3841 gcagcgcccg gcgatccgga cgccaccgat ggcccatggc cgcacccgat gctcctgccg
     3901 cgagctccac ggccgcgcca gcgccggtga gctggtcgag gttgcagccg ccgtcgtccg
     3961 tcgccgttgt tgccgtcgtc gcggccaagc ccgcgaagct ccccaaccaa gggttagttc
     4021 cctggcccgc agcgcggcac ggcccggtcc ggagtacaga catgtccggt ctgaaccgga
     4081 cgccaactgc aacgaacgtc acaacgaaac gaatctggta caggtctcgc cccgccgcga
     4141 ggtgacgcgc atcgccgcgc cgccgggcgc gaagctcgag gtggtcggct cgtaactgcg
     4201 cgagattgct cgcggaaaaa actgcatcgc gatcgtcgat ccggcggcgc gccgatcgtc
     4261 gtctcggaca gaccaaccga gtcacaagga gacacgcgat gaaggtcatg ttgttcgcga
     4321 tcgcaaccaa gcaaaccgaa gcgggccggc ctcctaccaa ggaggcggtg ctcgcgatgc
     4381 acaagttcaa cgaggagctg gagaaggccg
//
LOCUS       DASVOR010000686         3119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211175,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000686 DASVOR010000000
VERSION     DASVOR010000686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211175"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1961)
                     /locus_tag="VF516_13585"
     CDS             complement(<1..1961)
                     /locus_tag="VF516_13585"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8108759.1"
                     /translation="MSNAKFRAGMVGAGNICEFHVAAVKKLAPDVELVGVTDLDAARA
                     QANAEKWGTTAYKDMDALIAAGANVIHVLTPPAAHARVAIAALERGCHVLIEKPIAED
                     VDDARRVGALARQKGLTASVNHSLLYDPQVKRALDAVAAGALGQVVSVDILRGSEYPP
                     YEGGPLPPWYRDAGYPFRDIGVHCLYLIQALLGPIEDVDAEWSSLGGDPNLAFDEWRA
                     MVRCARGLGQFQLTYNTRPLQSQLIIHGTRSVLRVDLFAMFHGKRSNTPLPKAAERLV
                     NAFADSIQPLIDVPINVWKFVKKEVQAYQGLRDLVAEFYRRLAAGEPPPVSVDDAAQV
                     VVWVEKVARAADAEHRDRLAKFTLSDKVPFLVTGASGSLGRATVKRLRADGHRVRVFQ
                     RRIPTIPEDGVEYCFGNLGDPEAVDRAVRGAEVVIHCGAAMKGGWPEHKGGTVVGTQN
                     VIDACKKHKVRQLVHISSMSVIDWAGSAGNGPVSEAANLEPRPDERGAYTRAKLEAEQ
                     LVASAAAAGLPAVILRPGQIFGGGIPLINGAVARSAGGRWLVLGDGKLELPLVYIDDV
                     VDAIMAAVAKQLTRGEVIQIIDPEHLTQEDVLGLAGGNKAVLRVPRPVVFALGKLSEY
                     PLGALGRPSPVAVYRLKSALARLHYDSDRA"
     gene            complement(1958..>3119)
                     /locus_tag="VF516_13590"
     CDS             complement(1958..>3119)
                     /locus_tag="VF516_13590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8108760.1"
                     /translation="AKDWHEDPTSNHHVLRELARTRRVLWLNSLATRTPKLSSGRDLG
                     KIRRKLAEFTRGPENVENDLWVFTPLVLPLPASPLARTVNRQILRATIRALRLRLGIA
                     RFELWTFLPNTADYVGTLGEDRAVYYCVDEWSMFSYLDRAETAAAERRLLERVDAVFA
                     INHALADAKRQVNPATFVSPHGVDHALFARALDPALAVPADLAALPRPRIGFYGTLRD
                     WVDFELLAHIARARPAWSIVLLGQVLCDVSAVRGLPNVHLLGQKRHDELPAYCKGFDA
                     GMIPYRLDERMKFVNPLKLREYLAAGVPVVSTPLHEVVRYAHLCRVAGTPEAFVAALD
                     EAIAEADPATRRGRSHAMAKETWSARVAEVMRILDEMAQRDQAQQREQTEMR"
BASE COUNT          445 a         1062 c         1159 g          453 t
ORIGIN      
        1 gcgcgatcgc tgtcgtagtg caggcgggcg agcgcgctct tgagccggta gaccgcgacc
       61 gggctcggcc ggccgagcgc gccgagcggg tactcgctga gcttgccgag cgcgaacacg
      121 accgggcgcg ggacgcgcag cacggccttg ttgccgccgg ccaggccgag cacgtcctcc
      181 tgggtcaggt gctcggggtc gatgatctgg atgacctcgc cgcgggtgag ctgtttggcg
      241 accgcggcca tgatcgcgtc gaccacgtcg tcgatgtaga ccagcggcag ctcgagcttg
      301 ccgtcgccga gcaccagcca gcggccgccg gcgctgcgcg cgaccgcgcc gttgatcagc
      361 gggatgccgc cgccgaagat ctggccgggg cggaggatga cggcgggcag gccggcggcg
      421 gcggccgagg cgaccagctg ctcggcctcg agcttggccc gggtgtaggc gccgcgctcg
      481 tcgggccggg gctcgaggtt ggcggcctcg ctgaccgggc cgttgccggc cgagcccgcc
      541 cagtcgatga cggacatcga cgagatgtgg accagctggc ggaccttgtg cttcttgcag
      601 gcgtcgatca cgttctgggt gccgacgacg gtgccgccct tgtgctcggg ccagccgccc
      661 ttcatcgcgg cgccgcagtg gatcacgacc tcggcgcccc ggaccgcgcg gtcgacggcc
      721 tccggatcgc cgaggttgcc gaagcagtac tcgacgccgt cctcggggat cgtcgggatc
      781 cggcgctgga acacgcggac gcggtggccg tcggcgcgca gccgcttgac cgtggcgcgg
      841 ccgagcgagc ccgacgcgcc ggtgaccagg aacggcacct tgtcggacag ggtgaacttc
      901 gcgagccgat cgcggtgctc ggcgtcggcg gcgcgcgcga ccttctcgac ccagacgacg
      961 acctgcgcgg cgtcgtcgac ggacaccggc ggcggctcgc cggccgcgag ccggcggtag
     1021 aactcggcga ccaggtcgcg caggccctgg taggcctgga cctccttctt gacgaacttc
     1081 cagacgttga tcgggacgtc gatcagcggc tggatcgagt cggcgaacgc gttgaccagc
     1141 cgctcggcgg ccttgggcag cggcgtgttg gaccgcttgc cgtggaacat cgcgaacaga
     1201 tcgacgcgca gcacgctccg ggtgccgtgg atgatcagct ggctctgcag cggccgcgtg
     1261 ttgtaggtca gctggaactg gccgaggccg cgcgcgcagc gcaccatcgc gcgccactca
     1321 tcgaacgcga ggttgggatc gccgcccagc gagctccact cggcgtcgac gtcctcgatc
     1381 gggccgagca gcgcctggat caggtacagg cagtgcacac cgatgtcgcg gaacggatag
     1441 ccggcgtcgc ggtaccacgg cggcagcggg ccgccctcgt agggcgggta ctccgagccg
     1501 cgcaggatgt cgaccgagac gacctggccg agcgcgccgg cggcgaccgc gtcgagcgcg
     1561 cgcttgacct gcgggtcgta gagcagcgag tggttgacgc tcgcggtcag gcccttctgc
     1621 cgggcgagcg cgccgacccg gcgggcgtcg tcgacgtcct cggcgatcgg cttctcgatc
     1681 aggacgtggc agccgcgctc gagcgccgcg atcgcgaccc tggcgtgcgc ggcgggcggg
     1741 gtcaggacat ggatcacgtt ggcgccggcg gcgatcagcg cgtccatgtc cttgtacgcg
     1801 gtggtgcccc acttctcggc gttggcctgg gcgcgcgcgg cgtcgagatc ggtgacgccg
     1861 accagctcga cgtcgggcgc gagcttcttg accgcggcga cgtggaactc gcagatgttg
     1921 ccggcgccga ccatgccggc acgaaacttt gcattgctca tcgcatctcc gtctgttctc
     1981 tctgctgtgc ctgatctctc tgggccatct catccaggat gcgcatcacc tcggcgacgc
     2041 gcgcggacca ggtttctttc gccatcgcat gggagcggcc gcgccgggtc gccggatcgg
     2101 cctctgcgat cgcctcgtcg agcgcagcga cgaaggcctc cggcgtgccg gccacgcggc
     2161 acagatgggc gtagcgcacg acctcgtgca acggggtcga gaccaccggc acgccggccg
     2221 ccaggtactc gcggagcttc aacgggttga cgaacttcat ccgctcatcg aggcgatacg
     2281 gaatcattcc ggcgtcgaag cccttgcaat aagcgggcaa ctcatcgtgg cgcttctggc
     2341 cgagcaagtg aacgttggga agcccgcgca ccgccgagac atcgcacagc acctggccga
     2401 gcagcacgat cgaccacgcc gggcgggccc gcgcgatgtg cgcgagtagc tcgaaatcga
     2461 cccagtcgcg caaggtgccg tagaacccga tgcgcggccg gggcagcgcg gcgaggtcgg
     2521 ccggcaccgc cagcgccggg tcgagcgcac gcgcgaacag cgcatggtcg acgccgtgcg
     2581 gcgacacgaa cgtcgcgggg ttgacctggc gcttggcgtc ggccagggca tggttgatcg
     2641 cgaacaccgc gtcgacccgt tcgagcaggc ggcgctcggc ggccgcggtc tcggcgcgat
     2701 cgaggtagga gaacatcgac cactcgtcga cgcagtagta caccgcgcgg tcctcgccga
     2761 gcgtgccgac gtagtccgcg gtgttgggca ggaacgtcca cagctcgaac cgagcgatgc
     2821 cgagccgcag ccggagcgcg cggatcgtgg cgcgcaggat ctggcggttg accgtgcgcg
     2881 cgagcgggct ggccggcagc ggcaggacca gcggcgtgaa cacccacagg tcgttctcga
     2941 cgttctcggg cccgcgcgtg aactccgcca gcttgcgccg gatcttgccg aggtcgcggc
     3001 ccgacgacag cttcggcgtg cgggttgcta gcgagttgag ccacagcacc cggcgggtgc
     3061 gcgcgagctc gcgcaggacg tggtgattcg aggtcggatc ctcgtgccag tccttcgcg
//
LOCUS       DASVOR010000687         3834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415136,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000687 DASVOR010000000
VERSION     DASVOR010000687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415136"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1273)
                     /locus_tag="VF516_13595"
     CDS             complement(62..1273)
                     /locus_tag="VF516_13595"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8108761.1"
                     /translation="MSVIQAFHRTSVPALAGSDSIEHQLAALHALQLEHPFWNCRLLT
                     AFAQGALSRDDVRYVFSQYHLYSSSFTRFIAAVMANSESDLHRAQLAQNLWEEGGGCE
                     PSRRHAQIFRNFLNSSLGIDDVSRIVYAPYTRYFVREYLAQCQRSEPVWGTAFLSLGT
                     EGIVSRMYETIRTGLLLAGIPAGELEFFDIHIACDDDHALTLENMMMSYAGQPGWFDA
                     CLGAMRRALDLRMEFFEAIFEDLQRMRVEPIVLRMQNRESLARGLDDSELCHRQGGTT
                     IAMYDNEVARLNIRFTVERLPLAAEVLDPRMVRIPPGKFNEKHKHAHETLIHILEGSG
                     QVLIDDRVLPVSAGDSILVPRWAMHQTQNLGDTEMRFLAVTDFRLSQRAFVGDATDYR
                     MNADIDAKLRS"
     gene            2047..3021
                     /locus_tag="VF516_13600"
     CDS             2047..3021
                     /locus_tag="VF516_13600"
                     /inference="COORDINATES: protein motif:HMM:NF013810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108762.1"
                     /translation="MTRRLSWILVVLASAGAGCAQGGPGDPGDLESTTVQGVTFNGPA
                     NQFAGGFNPSNAPDGGFGGGSCTASRTPVIFVHGNSVNATFFAKPTTNGAPTVYATLK
                     AAGYNDCELFGITWLNPTEQANQQLNFHTATKAAMIRDFITAVKAYTGKSKVNILAHS
                     MGVTVALHGITFGNLWGSIDTFVNVAGGLRGLSSCLSVGFANPAFTTCGSQNVFDSNT
                     FGFWPDGAAGASNPRTGTSSSVGFRNEPARRTAVRFFTISGGSSDAIVGGVQCQFNAS
                     AQLKAQLNVAVDHLSSFTNTGAIMNTMLNTACTGTACCAGYSGSCTNL"
     gene            3050..>3834
                     /locus_tag="VF516_13605"
     CDS             3050..>3834
                     /locus_tag="VF516_13605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8108763.1"
                     /translation="MRFGIFMAPFHPAGVNPTILIDGDLQLIEHLDRLGYEEAWIGEH
                     HSAGSEIIASPEIFIAAAAERTRRIKLGTGVVSASYHHPLWVAERAVQLDHMTKGRFM
                     LGLGPGALPSDAAMIGLDMPQTRELLEDAVDVITRLMRSDEPVNFENSRWTLRNARLH
                     LRPYTHPHPEIAIAAVASPSGPRLAGRYGHGLLSVGATTAAGFDVLALHWNVMEERAA
                     HYGVTVDRNAWRLVGLVHLAETREQAYRDVEHGILQWFDYFQNT"
BASE COUNT          626 a         1354 c         1267 g          587 t
ORIGIN      
        1 caccgacggc cacggtaggt tcgtccagtc gctggggtag cgccccgccg tccgccgtcg
       61 ctcagctccg gagcttggcg tcgatgtcgg cgttcatccg gtagtcggtg gcgtcgccca
      121 cgaacgcgcg ctggctgagc cggaagtcgg tgaccgcgag gaaccgcatc tcggtgtcgc
      181 cgaggttctg ggtctggtgc atcgcccagc gcgggaccag gatcgagtcg ccggccgaca
      241 ccggcagcac gcggtcatcg atcagcacct gcccgctgcc ctcgaggatg tggatcagcg
      301 tctcgtgggc gtgcttgtgc ttctcgttga acttgccggg cgggatccgc accatgcgcg
      361 gatcgagcac ctcggcggcg agcggcagcc gctcgaccgt gaaccggatg ttgagcctgg
      421 cgacctcgtt gtcgtacatc gcgatcgtcg tgccgccctg gcggtggcac agctcgctgt
      481 cgtcgagccc gcgcgccagc gactcgcggt tctgcatgcg cagcacgatc ggctcgaccc
      541 gcatccgctg gaggtcctcg aagatcgcct cgaagaactc catccgcaga tcgagcgcgc
      601 gccgcatcgc gccgaggcat gcgtcgaacc agcccggctg gccggcgtac gacatcatca
      661 tgttctccag cgtgagggcg tggtcgtcgt cgcacgcgat gtggatgtcg aagaactcga
      721 gctcgcccgc ggggatcccg gcgagcagca ggccggtccg gatggtctcg tacatccgcg
      781 acacgatgcc ctcggtgccg agcgacagga acgcggtgcc ccacaccggc tcggagcgct
      841 ggcactgggc caggtactcg cgcacgaagt accgggtgta aggcgcgtag acaatccgcg
      901 agacgtcgtc gatgccgagc gagctgttca ggaagttgcg gaagatctgg gcgtgacggc
      961 gcgacggctc gcagccgccg ccctcctccc acaggttctg cgccagctgg gcgcggtgca
     1021 ggtcgctctc cgaattggcc atcaccgcgg cgatgaaccg cgtgaagctc gacgagtaga
     1081 ggtgatactg cgagaacacg taacggacgt cgtcccgcga cagcgcgccc tgcgcaaacg
     1141 cggtgagcag ccgacagttc cagaacgggt gctccagctg cagcgcgtgc agcgccgcga
     1201 gctggtgttc gatggaatcg ctgcccgcga gcgcaggaac cgacgtacga tgaaatgcct
     1261 ggatgacgct cactactgtg ctccttcttg gtgcccgatg gcaggtaccg atgcgaacgg
     1321 tcgcctgagc acttcgcgtg ccgggcgatc gatcacacgg cagccggact tcgccatgcg
     1381 tggcggatcg ttcgccggac cgtctggcgc ataaccgcgt ggagcgcgcc gacaaatcgc
     1441 cgccgtgcga acgcgctcac gacgcctgtc cccggaagct gcggtcgcca cccctggtca
     1501 ctcgcttgac catgaacgat gacgcatgtg ccggggaaac cactacacgg ggcctgggcg
     1561 ctcgcggtcg acgaggtcta cccggcgatt tccgtcgagc cgtcatgttg ccgtccggta
     1621 tccggacaca cggaaccggg tgtcgggcat ccatggcggt cgccggcgcc gggatgtgcg
     1681 acgccgacgc tcgcagagca cgcgcgcgtg cgaatcggcc gaacgatacg ggcgtgccgc
     1741 tgatcacgaa cagcgcggtt gtgtacctgg cgcacaccga cggcgttgca gtcccggcgc
     1801 aacgcccgcc ggacctcggc tcgtgaccca cggcgatccg ccccgtcctg ccccatgacg
     1861 cgatccgctg cacctgctgc gtcaccgccg acgctcgcag atcgtgccag ccgcgtcaca
     1921 tacgacgcac gacgcgcacg actctccagg acttggtgat agcttcgcgt ggcctgcgcc
     1981 gtgccagaga tccgggcccg atcgggccac tggcaaccac gaaggagcga aacgatgaca
     2041 ccattcatga cacggcggct ctcctggatc ctcgtcgtcc tggccagcgc cggggccgga
     2101 tgtgcgcagg gggggcccgg cgatcccgga gatctggagt ccaccaccgt ccagggcgtg
     2161 accttcaacg gcccggccaa ccagtttgcg ggcggcttca acccgtccaa cgcgcccgac
     2221 ggcgggttcg gcggcggcag ctgcacggcg agccggaccc cggtgatctt cgtccacggc
     2281 aacagcgtga acgcgacgtt cttcgccaag ccgacgacca acggcgcgcc gacggtctac
     2341 gccacgctga aggccgcggg ctacaacgac tgcgagctgt tcggcatcac ctggctcaat
     2401 ccgaccgagc aggccaacca gcagctcaac ttccacaccg ccaccaaggc ggcgatgatc
     2461 cgcgacttca tcacggcggt gaaggcctac accggcaagt cgaaggtcaa catcctcgcg
     2521 cactcgatgg gtgtgaccgt cgcgctgcac ggcatcacgt tcggcaacct gtggggcagc
     2581 atcgacacct tcgtcaacgt cgcgggcggc ctccgcgggc tgtcgagctg cctgtcggtc
     2641 ggcttcgcca atcccgcgtt caccacgtgc ggcagccaga acgtgttcga ctccaacacc
     2701 ttcgggttct ggccggacgg cgccgccggg gcgtcgaatc cccgcaccgg caccagcagc
     2761 tcggtcgggt tccgcaacga gccggcgcgg cggaccgcgg tgcggttctt cacgatctcc
     2821 ggcggcagct cggacgcgat cgtcggcggt gtgcagtgcc agttcaatgc gtccgcacag
     2881 ctcaaggccc agctcaacgt cgccgtcgat cacctgtcgt cgttcaccaa caccggagcg
     2941 atcatgaaca ccatgctgaa cacggcgtgc accggcacgg cgtgctgcgc gggatactcc
     3001 ggatcctgca ccaacctgta agccctggga ggcagtgcgg atgtcgcgta tgcgtttcgg
     3061 aatcttcatg gccccgttcc accccgccgg ggtcaatccg accatcctga tcgacggcga
     3121 tctccagctg atcgagcacc tcgaccggct gggctacgag gaggcatgga tcggcgagca
     3181 ccactccgcc ggcagcgaga tcatcgcgtc gcccgagatc ttcatcgccg cggcggccga
     3241 gcgcacccgc cggatcaagc tgggcaccgg cgtcgtcagc gcgagctatc accacccgct
     3301 gtgggtcgcc gagcgcgcgg tccagctcga tcacatgacg aagggccggt tcatgctggg
     3361 cctgggcccc ggcgcgctgc cgagcgacgc ggcgatgatc ggcctcgaca tgccgcagac
     3421 ccgcgagctg ctcgaggacg cggtcgacgt catcacgcgg ctgatgcgca gcgacgagcc
     3481 ggtcaacttc gagaacagcc ggtggacgct gcgcaacgcc cggctgcacc tccgcccgta
     3541 cacccatccg caccccgaga tcgcgatcgc cgcggtcgcc tcgccgtccg gtccgcggct
     3601 cgccggtcgc tacggtcacg gcctgctctc ggtcggcgcc accacggccg cggggttcga
     3661 cgtgctcgcg ctgcactgga acgtcatgga ggagcgcgcg gcgcactacg gcgtcaccgt
     3721 cgaccgcaac gcgtggcggc tggtcggcct ggtccacctc gccgagaccc gcgagcaggc
     3781 ctaccgggac gtcgagcacg gcatcctcca gtggttcgac tacttccaga acac
//
LOCUS       DASVOR010000688         7109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_182191,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000688 DASVOR010000000
VERSION     DASVOR010000688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7109
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182191"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            816..1724
                     /locus_tag="VF516_13610"
     CDS             816..1724
                     /locus_tag="VF516_13610"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8108764.1"
                     /translation="MTTPVPDERMRLIGTGKPLEAGILARMEGFFGADFSGVRVHEGP
                     IAEQMGALAFTLGETLYFSPGLYNIRTREGLELLGHELAHVVQQRDGRVANPYGRGVA
                     IVQDPSLEAEADLMGQRAAKAILSGTGIGQAAMGSRPGAAVTDPDVLDADMPTESLDP
                     ALQLRHLKHQYNSLRGTSASVAATIVSGVNTARGSISVNGQQVASATGFSGHSMRRSW
                     VSREARRMNRGGFNGAARQADAEIQVLLRLEDQIEEEDLDVYGGHLYINSDLEICRVC
                     RRCIRVFCRYYGLTDHMSSHQESTLY"
     gene            2033..2566
                     /locus_tag="VF516_13615"
                     /pseudo
     CDS             2033..2566
                     /locus_tag="VF516_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
     gene            2662..3315
                     /locus_tag="VF516_13620"
     CDS             2662..3315
                     /locus_tag="VF516_13620"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEX8108765.1"
                     /translation="MSIQTIERPAGINVYGSQLVRVDPDFAILDFSVIATEATPALAF
                     SKAREAAAAVRSFVGNSSIPDADVRTSQMSLEQAFEHQGGQRRAVGFTAKVGFQVVTE
                     ALGRVELLLAGVVDAGANQVDRVTFQTSRLREIRTEARRQAFARAKAKAELFALAAGV
                     RLGHVLHVEDINPDQYGAHRHYQADVDMTDEDPERTATNPGSIVVNAAVMVCFAILP"
     gene            complement(3401..4747)
                     /locus_tag="VF516_13625"
     CDS             complement(3401..4747)
                     /locus_tag="VF516_13625"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8108766.1"
                     /translation="MLPPGPSSHPFLNMVRWVRRPAPMLEQLAATYGPAFSVRLPGMP
                     EPMVFFSDPAAIKDIWTGDPDVLRAGEANAILRSALGPSSLLLLDGLEHLRERRLMLP
                     PFHGDRMRAYGDAMRDATLAELARWKHGVAFPIHGAMQTITLEVIMRTIFGVGDEPRL
                     RPLRDALVRYTTLGTSRVGTAVLLLTPAKYAVRVQQAAAHGHGRLLPWSPIVRAQSET
                     DRIVRDVIAARRRGGTAGRTDVLSMLLDARDEHGAAMTDDELRDEMLTLLLAGHETTA
                     TTLAWVIHHLLAHPPHLARVRAEVDEAGDDVERMVFLDAVIKETLRLTPIIPLVARHL
                     ARAATIGGHALPAGAVAAAAIYLVHRRPDLWPDPARFDPTRFAGKKPDPTHYFPFGGG
                     TRRCLGMAFAIFEMKIVLATILARVDLAAASDRVRLVRRGITLAPSGGMPIVARPR"
     gene            complement(4784..5356)
                     /locus_tag="VF516_13630"
     CDS             complement(4784..5356)
                     /locus_tag="VF516_13630"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8108767.1"
                     /translation="MVEPASSYVAYEEYLAAEQKSDTKHEWLDGVIYAMAGGTLEHSR
                     LAQNLGAALKTALSGCDVFQSDAMTFVRATQLSTYADVAVVSGPVESQKIERNGRVLG
                     EALLNPTVLVEVLSETTEEYDRGEKFAHYMRIPSLREYVLVSQGTPRIEVFRRPERGH
                     WIHQEARAGGTIAIAGRTIHVDDIYRRASA"
     gene            complement(5419..6090)
                     /gene="rpiA"
                     /locus_tag="VF516_13635"
     CDS             complement(5419..6090)
                     /gene="rpiA"
                     /locus_tag="VF516_13635"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF001924.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEX8108768.1"
                     /translation="MTDDFKRQAAHAALAELPPDGVIGIGTGSTARLFIAALGELVAA
                     GRRYTCVPTSEASRIQAEGLGIPLLSDDGPWDIAVTVDGADEVDDALDLIKGGGGAHT
                     REKIVNFSSRRNVIVVDASKRSRRIGEKWAVPVEVLPFGHLATRAHLARLGAPTLRLR
                     DGAPFRTDAGNLIYDVACGPIADPRALDAALHAIPGVVETGLFLGRADVVLIAGTTGV
                     ERRAR"
     gene            complement(6087..>7109)
                     /locus_tag="VF516_13640"
     CDS             complement(6087..>7109)
                     /locus_tag="VF516_13640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108769.1"
                     /translation="RGSRRRIAGRRARFSAGGCYGPWMRTAGILAAHVLSTAALHGPA
                     RADTARAWAAAKAGLPADTKVVIGIDIATVQKTQLFATFYPKLHDQPDIAKVLDAMKD
                     GCKIDPLAVIQGLVVASSGEQDDGAMYLAISGIDRNKLSSCLQTTAQANAPAAEAGDK
                     PKVTIKQTGNITELSRGGDSGYFGWVGTNVLIVAFHATDKASLTRWMGGKGALAKSDV
                     GKALARVNTSAAMWGAGTGTKELQPGMTAKGGYGAIAYAKGQLSVDIHAVMESAAQAG
                     SASMMANQQLNLIKATGQLPAEIAPVIQAITVSADNDEVRIKGSVAEKDVLGAVAFAI
                     NNFGSP"
BASE COUNT         1113 a         2311 c         2584 g         1101 t
ORIGIN      
        1 ggccgacgcg gccaagcccg ctgcgcacac gcccgccaag ccgtagcgtc gcctcacccc
       61 tcgcgaggga ttcccgggat ggccgacccc ctggccattg gctgaaaacg ctcggtcgcg
      121 ccggagctcg aaagccagcc accatgggcg atcggctcac tcgcgagtca gccagtcgtt
      181 cccgccggcc gacagcccgg cctgcggctg ccgcagcttg gccgaaaatt ggcccgcctg
      241 cgcaccgggg ataccatggc gtcgtcgcag ggcggaccga gcgcctcgcc gcgctgcaag
      301 accatcgcgg ccgtgatggg cacacgcgac gggtgactgt cgctgcaacc acgagggggt
      361 ttccaacgat gttcacgaac cggtcgcaac agagcatggt gttgcagggc tacttccgca
      421 tcgagcgggt tcgcggttgc gggtgtcaga ccgcagcggt tcagcccagg agccggacgg
      481 actgggcagc gaaggcgccg cggccggatc tgatgcccgc gtggaagcca cgtggagcgg
      541 cgcaggccat gatggccgcc gggacaggat ggcgcggggc gccgcgaccc gatctgatgc
      601 ccgcgtggaa gcccagtgga gcggcgcagg ccaaggcacg agccccgcgg cctgatccga
      661 tgcccgcatg gaaggcaagc ggagcggcgc aggccaatgc agcggcgggg gcggcgtggc
      721 ccggggcacc gcgacccgac ctgctgcccg gaatgggcaa gccgaggatt ggcttgccgg
      781 gtggggcagt gcaagcgcgg gccaaccagg gtgtcttgac aacgccggtg ccggacgagc
      841 ggatgcggct gattggcact gggaagccgc tcgaggccgg gatcctggcg cggatggaag
      901 gattctttgg tgccgacttt tcgggtgtgc gggtgcacga ggggcccatc gcggagcaga
      961 tgggggcgct ggcattcacg ctgggagaga cattgtattt ttcgccgggg ctttataata
     1021 tcaggactcg cgagggactg gagttgctcg ggcacgagct ggcgcatgtg gtgcagcaac
     1081 gggatgggcg ggtggcaaat ccctatggcc gtggggtggc gatcgtgcag gacccgagcc
     1141 tggaggcgga ggcggacctg atggggcagc gagcggccaa ggcgatcctg tcagggacgg
     1201 ggatcgggca ggcggcgatg ggatctcgtc cgggtgcggc agtgactgat cctgatgtac
     1261 ttgatgcaga tatgcccaca gagtcgcttg atccggctct gcagctacgt cacctcaagc
     1321 atcaatacaa ctccttacgt ggcacgtcag cctcggtggc ggcgaccatc gtatcgggcg
     1381 tcaacaccgc tcgtggctcg atctccgtga atggacagca ggttgcgtcc gcaactggtt
     1441 tcagcggaca ttccatgaga cgctcctggg tgtcccgtga agccaggcgc atgaacaggg
     1501 gaggcttcaa cggcgccgca cgacaagcgg acgctgagat tcaagtgcta ttgaggcttg
     1561 aagatcagat cgaagaggaa gatctggacg tctatggtgg gcacctgtat attaactcgg
     1621 atctggagat ctgtcgggtc tgtcgccggt gcatcagagt cttctgcagg tattacggct
     1681 tgaccgatca catgtccagc catcaagaaa gcactctgta ctgacaggag gcgctgccag
     1741 tcttgccggg tctggctccg ggatccgcgg tcggggccag gcgacaacga acacgctcgc
     1801 ccgtgggtgc agatcgcggc aggtcctgcc cggccacggg cggcggttca ccgcgccgga
     1861 cgcggaggcg atgcgggcgg cggtgctacg ggcggcggcg tgaaacggca gacgagccgg
     1921 ccgtaaccgc tcgcaccgaa gggacgtata ctgaggcatg ctgcgtccac tcgaccgcac
     1981 gtcgccggcg acccagaccg cgaccttcgg cctcggttga ttctggggtc ccgatgcccg
     2041 gttcgggtgt caccctgcga tcgttcgcac gtgcgtcggc tacgccggcg gcagcaccgt
     2101 gaacccgacg taccacgacc tcgccgatca caccgaggtg ttccaggtcg acttcgaccc
     2161 tgcgaagctg tcgtacgagg agctgctgcg gcagatctgg gcgaaccggc gcggcggacc
     2221 gagctaccgc ggacagtaca tggaggcggt gttctgcatg gacgccgagc aggaggcgac
     2281 cgcgcggcgg ctcgggatcc gcgcaccgat catccccggc gcgcggttct atctcgccga
     2341 ggattaccac cagaagtact acctccggca cgagcgcgcg ctgatcgcgg agctcgccga
     2401 ctacacgccg cgccagctcg tcgagtcgac ggtcgccgct cggctcaatg gctacgtctc
     2461 cgggcgcggc acactcgcgc agctgcatga cgagctgccg cagcttgggc tgtccgcggc
     2521 ggcggcgacc catctcgagc ggctggtcgc ctcgcgctcg cgatgagcca acgcagtgta
     2581 atcactcgac aacggcgtgt cttccgacga cgcgacgcga ggcgatgatc ctgtcaacga
     2641 tgggcactat gctcttcatc gatgagcatc cagacgatcg agcgtccagc aggtatcaac
     2701 gtgtatggtt cgcagctggt acgggtcgat cctgacttcg cgatcctcga cttttccgtg
     2761 atcgcgaccg aagccacgcc cgcgctggcg ttctccaagg cacgagaagc cgccgcagcg
     2821 gtacgaagct tcgtcgggaa ctccagcatc ccggacgcag atgttcgcac ctcgcagatg
     2881 tctctcgagc aagccttcga gcatcaaggt ggccagcgac gagcggtcgg gttcaccgcg
     2941 aaggtcgggt tccaggtcgt gaccgaggcg ctcggtcgcg tcgagctctt gcttgcggga
     3001 gtggtcgacg ccggcgccaa tcaggtcgat cgagtcacgt tccagaccag ccggcttcgc
     3061 gagattcgca ccgaggcgcg ccgccaggcg ttcgcgcgcg ccaaagcgaa ggccgagctc
     3121 tttgccctgg ctgcaggcgt gagactcggc catgtgctcc acgtggagga catcaacccg
     3181 gaccagtacg gtgctcaccg ccattatcaa gccgacgtgg acatgaccga cgaagatccg
     3241 gagcgcaccg ccacgaaccc cggaagcatc gtcgtgaacg ctgcggtcat ggtgtgcttc
     3301 gcgattcttc cgtgatcccg ctcgacgggc gtagaagtcg gacgagcgct ggacacccct
     3361 gcgtgtaggc gttctgctgg gcgctacacg cgacacactc ctaacgcggc cgcgcgacga
     3421 tgggcatgcc gcccgacggc gcgagcgtga tgccgcggcg caccagccgc acgcggtccg
     3481 aggctgcggc gaggtcgacg cgcgcgagga tcgtcgcgag cacgatcttc atctcgaaga
     3541 tcgcgaacgc catcccgagg cagcggcgag tgccaccgcc gaacgggaag taatgggtcg
     3601 gatcgggctt cttgccggcg aagcgcgtcg ggtcgaaccg cgcggggtcg ggccacaggt
     3661 cggggcgacg gtgcacgagg tagatcgccg cggcggcgac ggcgcccgcg ggcaaggcgt
     3721 gaccgccgat cgtggccgcg cgcgcgaggt ggcgagcgac gagcgggatg atcggcgtga
     3781 ggcgcagggt ctccttgatc accgcgtcga ggaagaccat gcgctcgacg tcgtcgccgg
     3841 cctcgtcgac ctcggcgcga acgcgcgcga ggtggggcgg atgcgcgagc aggtgatgga
     3901 tcacccaggc gagcgtggtc gccgtcgtct cgtgccccgc gagcaggagc gtcagcatct
     3961 cgtcgcgcag ctcgtcgtcg gtcatcgccg caccgtgctc gtcacgcgcg tcgagcagca
     4021 tcgagagcac gtcggtgcgg cccgcggtgc ccccgcggcg gcgggcggcg atcacgtcgc
     4081 gcacgatgcg atcggtctcg gactgcgcgc ggacgatcgg cgaccacggc agcaggcggc
     4141 cgtggccgtg cgcggcggcc tgctgcaccc gcaccgcgta cttcgccggg gtgagcagca
     4201 ggacggcggt gccgaccctc gacgtgccca gcgtggtgta gcggacgagg gcgtcgcgca
     4261 gcgggcgcag ccgcggctcg tcaccgacgc cgaagatcgt acgcatgatc acctcgaggg
     4321 tgatcgtctg catcgcgccg tggatcggga aggcgacgcc gtgcttccag cgcgcgagct
     4381 cggcgagcgt ggcgtcgcgc atcgcgtcgc cataggcgcg catgcgatcg ccgtggaacg
     4441 gcgggagcat caggcggcgc tcgcgcaggt gctcgaggcc gtcgagcagc agcagcgagc
     4501 tcgggccgag cgccgagcgc aggatggcgt tcgcctcgcc cgcgcgcagg acgtcggggt
     4561 cgccggtcca gatgtccttg atcgccgcgg ggtcggagaa gaacaccatc ggctcgggca
     4621 tgcccgggag gcgcacggag aacgccgggc cgtaggtcgc ggcgagctgc tcgagcatcg
     4681 gcgctggcct gcgcacccag cgcaccatgt tgaggaacgg gtgcgagctc ggtccgggtg
     4741 gcagcacgcc acgagtgtag cgatgcccgg ccggctcacg ccactacgcg ctggcccgcc
     4801 gatagatgtc gtcgacgtgg atcgtccgtc cggcgatcgc gatcgtcccc ccggctcgcg
     4861 cttcctggtg gatccagtgg cctcgttccg gacgccggaa cacctcgatc cgcggcgtgc
     4921 cctgcgagac cagcacgtac tctcgaaggc tcgggatccg catgtagtgc gcgaacttct
     4981 cgccccggtc gtactcttcc gtcgtctccg agagcacctc gacgagcacg gttgggttca
     5041 gcaacgcctc gccgagaacg cggccgttgc gttcgatctt ctgcgactcg acggggccag
     5101 agacaaccgc cacatcggca tacgtcgata gctgggtcgc gcgcacgaac gtcatcgcat
     5161 cggactgaaa cacgtcgcag ccggagagcg cggtcttcaa ggccgcgccc aggttctgcg
     5221 cgaggcggct atgctcgagc gttccgcccg ccatcgcgta gatcacgcca tcgagccact
     5281 cgtgcttcgt gtcgctcttc tgctccgccg cgaggtactc ctcgtaggcg acatagctcg
     5341 acgcgggttc aaccatcgga ccgattgtac catcgacgcc cggaggtgca gcgcaatgga
     5401 cgtgcgggac ggacgccgtc agcgcgcgcg gcgctcgacg cccgtggtgc cggcgatcag
     5461 gacgacgtcg gcgcggccga ggaacaggcc ggtctcgacc acgccgggga tcgcgtggag
     5521 cgcggcgtcg agcgcgcgcg ggtcggcgat cgggccgcac gcgacgtcgt agatcaggtt
     5581 tccggcgtcg gtgcggaacg gggcgccgtc gcggaggcgc agggtcggtg cgccgagccg
     5641 ggcgaggtga gcgcgggtgg cgaggtggcc gaacggcagg acctcgaccg gcacggccca
     5701 cttctcgccg atccggcgcg atcgcttgga cgcgtcgacg acgatgacgt tgcggcgcga
     5761 gctgaagttg acgatcttct cgcgggtgtg ggcgccgccg ccgcccttga tcaggtcgag
     5821 cgcgtcgtcg acctcgtcgg cgccgtcgac ggtgaccgcg atgtcccacg ggccatcgtc
     5881 ggagagcagc gggatgccga gcccctcggc ctggatccgg ctggcctccg aggtcgggac
     5941 gcaggtgtag cgccggccgg cggcgacgag ctcgccgagc gcggcgatga acagccgcgc
     6001 ggtcgaaccg gtgccgatgc cgatcacgcc gtcgggcggc agctcggcga gcgcggcgtg
     6061 ggcggcctgg cgcttgaagt cgtcggtcat gggctgccga aattgttgat cgcaaatgca
     6121 acggcgccca gcacgtcctt ctcggcgacg ctgcccttga tccggacctc atcgttgtcg
     6181 gccgacaccg tgatcgcctg gatcaccggg gcgatctcgg ccggcagctg gccggtcgcc
     6241 ttgatcaggt tgagctgctg attggccatc atcgatgccg atccggcctg cgcggcgctc
     6301 tccatcaccg cgtggatgtc gacggacagc tgacccttgg catacgcgat cgcgccgtag
     6361 ccgcccttgg cggtcatgcc gggctggagc tccttggtgc cggtgccggc gccccacatc
     6421 gcggcggagg tgttgacccg agcgagggcc ttgccgacgt cggacttggc gagcgcgccc
     6481 ttgccgccca tccaccgggt cagcgatgcc ttgtcggtgg cgtggaacgc cacgatgagg
     6541 acgttcgttc cgacccagcc gaagtagccg ctgtcgccgc cgcggctgag ctcggtgatg
     6601 ttgccggtct gcttgatggt gaccttgggc ttgtcgccgg cctcggcggc cggcgcgttg
     6661 gcctgggcgg tggtctgcag gcacgacgac agcttgttgc gatcgatgcc ggagatcgcc
     6721 aggtacattg cgccgtcgtc ctgctcgccc gacgacgcga cgacgaggcc ctggatgacc
     6781 gcgagcggat cgatcttgca gccgtccttc atcgcgtcga gcaccttggc gatgtcgggc
     6841 tggtcgtgca gcttggggta gaacgtcgcg aacagctggg tcttctgcac ggtggcgatg
     6901 tcgatgccga tcacgacctt ggtgtcggcg ggcagtccgg ccttggccgc ggcccaggcg
     6961 cgggcggtgt cggcgcgggc cgggccgtgg agagcggcgg tcgacaggac gtgggcggcg
     7021 aggatcccgg cggtgcgcat ccatggacca tagcagccgc cggccgagaa tctcgcgcgg
     7081 cggcccgcga tgcggcggcg ggagccgcg
//
LOCUS       DASVOR010000689         2894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_298932,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000689 DASVOR010000000
VERSION     DASVOR010000689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298932"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..643)
                     /locus_tag="VF516_13645"
     CDS             complement(107..643)
                     /locus_tag="VF516_13645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108770.1"
                     /translation="MAGRAARSRRNQVAAATPARPPASQRPGGPGGGAVHRDVRRWIY
                     AGLDLLFAAAYAFAIWKVIPNRLPSAAAHLWTFPGASLAMAIGMVLGGPRGWWIAVVA
                     GSAALASLFVLIVRILISAAFLAGVYGAFGKAAATFALVMVALVVELVALLPIVQVKY
                     LLTRSGRRAFGRTGREGS"
     gene            687..878
                     /locus_tag="VF516_13650"
     CDS             687..878
                     /locus_tag="VF516_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108771.1"
                     /translation="MQPILAESAPHRRPLVLAAVLTLAASIAAVAVAMPRPHHTRWHR
                     HHHLPYNGQCPYGYVYVAR"
     gene            complement(894..>2894)
                     /locus_tag="VF516_13655"
     CDS             complement(894..>2894)
                     /locus_tag="VF516_13655"
                     /inference="COORDINATES: protein motif:HMM:NF037016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pre-peptidase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8108772.1"
                     /translation="SPPVLSGLTDQVAQVGTELMVNLHATDPDGDALTYKFQAPDLTD
                     LDGHAAITVSPSGTGVFKWTPTQADVGLHAFDFTVADAGHTITGTIHIDVRSAIGTAT
                     APVFRQPLGSGTTLDLTQQTCVDVDIVIDAGSSTVRISQIDPVIDGAQLNVRDGHTAT
                     WHWCPTKAQQAQPRYMLVLSADDGDNPKVVKNYLVVLRGNSGTSCPGTPPVISHTPHD
                     VSSIVDLAIDATVTDDKGIKDAPLFYYSMTQPASPPDLSKMSQTTMKAQSGDRTNGVY
                     EATVPNPVAQMPAGTKQVLYYVIAATDNDDTTGTCNHTTTSQVYSMTVTSTGTANLPI
                     CAACTSDAQCGTGDECVRMGSSGATFCLQACGAGCPAGYTCSTTPVTSVDGASAPQCV
                     PQSGTCEMTTTSCVDDSWEVNDSLSQAKANPALTPGSYDLVSCPEPGDDFFSNDDWFR
                     IAVTGDSQIDLKLSGGTATDLDLFLNDANGNVIKRSVSLSSSEEVTTCLPAGTYYAEV
                     VGFGSARNPYKLIYTSTAQSCTAACVDDSHEPDSSAATARTAALPGFSSTGNQICPDN
                     DDWYKVRLFTGDLLVVDLTFNQSTPSQDLDIHLYKDGVDLTPCDASNPDTCAVDNGQS
                     GSSNEHAEFLITSPCDSGCDYDVVVRGFNHSANGYNIAITVE"
BASE COUNT          407 a          994 c         1034 g          459 t
ORIGIN      
        1 ggcgcgatcc ggaacgccag gtacacgacg gcggtggcgg cgaggcacac cgcgccgggc
       61 gcgatccaca ccgccgccag cgccagggcg cgcatcgcac gccgcgtcac gagccctcgc
      121 gcccggtccg gccgaacgcg cgacggccgg accgcgtcag caggtacttg acctgcacga
      181 tcggcagcag cgcgaccagc tcgaccacga gcgcgaccat caccagcgcg aacgtcgcgg
      241 cggccttgcc gaacgcgccg tacacgccgg cgaggaacgc cgccgagatc aggatccgga
      301 cgatcagcac gaacagcgac gccagcgccg ccgacccggc gacgacggcg atccaccagc
      361 cccgcggccc gccgagcacc atcccgatcg ccatcgccag gctcgccccc gggaacgtcc
      421 acaggtgggc cgccgccgac ggcagccggt tggggatcac cttccagatc gcgaacgcgt
      481 aggcggccgc gaacagcaga tcgagcccgg cgtagatcca ccgccggacg tcccggtgga
      541 cagcgccgcc gcccgggccc cccgggcgct gtgacgcagg cggccgtgca ggtgtcgcgg
      601 ccgcgacctg gttgcgtctc gagcgcgccg cgcggccggc catctcggag cgagcataag
      661 ccggagcgcg ctggtatcgt agggccatgc agccgatcct cgccgagtcc gcgccgcatc
      721 gccgccccct ggtgcttgcc gccgtgctca ccctcgcagc gtcgatcgcc gcggtcgccg
      781 tcgccatgcc gcgaccgcac cacacgcggt ggcatcgcca ccatcacctg ccgtacaacg
      841 gccagtgccc gtacggctac gtctacgtcg cgcgctagcg gcgtgcgcgc gtctcactcc
      901 acggtgatcg cgatgttgta gccgttcgcc gagtggttga agccgcgcac cacgacgtcg
      961 tagtcgcagc ccgaatcgca gggcgatgtg atcaggaact ccgcgtgctc gttggacgag
     1021 ccgctctggc cgttgtcgac cgcgcacgtg tctggattgc tcgcgtcgca cggcgtgaga
     1081 tcgacgccgt ccttgtagag atggatgtcg agatcctggc tcggggtcga ctggttgaac
     1141 gtgaggtcga cgaccaggag gtcgccggtg aacaggcgca ccttgtacca gtcgtcgttg
     1201 tccgggcaga tctggttgcc ggtcgacgag aagccgggca gcgccgccgt gcgcgcggtg
     1261 gcggccgagc tgtcgggctc gtggctgtcg tcgacgcacg ccgcggtgca cgactgggcg
     1321 gtgctggtgt agatcagctt gtatgggttg cgcgcgctgc cgaagccgac gacctcggcg
     1381 tagtacgtgc cggccggcag acacgtggtg acctcctcgc tggagctcag gctcaccgag
     1441 cgcttgatca cgttgccgtt cgcgtcgttc aagaacaggt cgaggtcggt cgcggtgccg
     1501 ccggacagct tgagatcgat ctgcgagtcg cccgtcacgg cgatccggaa ccagtcgtcg
     1561 ttcgagaaga agtcgtcccc cggctccggg cagctcacca ggtcgtagct gcccggggtc
     1621 agcgccgggt tggccttcgc ctggctcagg ctgtcgttga cctcccagct gtcatccacg
     1681 cagctcgtgg tcgtcatctc gcacgtgccg ctctgcggga cgcactgcgg ggccgacgcg
     1741 ccgtcgaccg aggtcaccgg cgtggtcgag caggtgtagc cggccgggca gcccgcgccg
     1801 cacgcctgca gacagaacgt tgccccgctc gaccccatcc gcacgcactc gtcgccggtg
     1861 ccgcactgcg catcgctggt gcacgcggcg cagatcggca ggttggccgt gccggtcgag
     1921 gtcaccgtca tcgagtagac ctgcgacgtc gtcgtgtggt tgcaggtgcc ggtggtgtcg
     1981 tcgttgtcgg tcgcggcgat cacgtagtac aggacctgct tggtgcccgc cggcatctgg
     2041 gcgaccgggt tgggcacggt cgcctcgtac acgccgttgg tgcgatcgcc gctctgcgcc
     2101 ttcatcgtcg tctggctcat cttcgacaga tccggcggcg aggccggctg ggtcatcgag
     2161 tagtagaaca gcggcgcgtc cttgatgccc ttgtcgtcgg tgacggtcgc gtcgatcgcg
     2221 aggtcgacga tcgacgacac gtcgtgcggc gtgtggctga tcaccggcgg cgtgcccggg
     2281 cagctcgtgc cgctgttgcc gcgcagcacg accaggtagt tcttgaccac cttggggttg
     2341 tcgccgtcgt cggcggacag caccagcatg tagcgcggct gggcctgctg cgccttggtc
     2401 gggcaccagt gccaggtcgc ggtgtggccg tcgcggacgt tgagctgcgc gccgtcgatc
     2461 accggatcga tctggctgat ccgcacggtc gacgagccgg cgtcgatcac gatgtcgacg
     2521 tcgacgcagg tctgctgggt caggtcgagc gtggtgccgc tgcccagcgg ctggcgaaac
     2581 accggggcgg tcgccgtgcc gatcgccgag cggacgtcga tgtggatcgt cccggtgatg
     2641 gtgtggcccg catcggccac cgtgaagtcg aacgcgtgga ggccgacgtc ggcctgggtc
     2701 ggggtccact tgaacacgcc ggtgccgctc ggcgacaccg tgatcgcggc gtggccatcg
     2761 aggtcggtga ggtcgggagc ctggaacttg taggtcagcg cgtcgccgtc gggatcggtg
     2821 gcgtggaggt tgaccatcag ctccgtgccg acctgtgcca cctgatcggt gagtccggac
     2881 agcaccggcg gcga
//
LOCUS       DASVOR010000690         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_298933,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000690 DASVOR010000000
VERSION     DASVOR010000690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298933"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(509..2131)
                     /locus_tag="VF516_13660"
     CDS             complement(509..2131)
                     /locus_tag="VF516_13660"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HEX8108773.1"
                     /translation="MRRTAIALVAITGIASAQPLPSDPRTLDGDPRLGDPRSTGVGPK
                     VPITLRPGGEVLGGDIVAPVSPLGLVPSEPPLSIEHPIDPETYVCGPGDVFELDFWGS
                     QNLQLKLTTDLEGRAFIPRVGFIPVAGKTLSVVRTAMKSKIRAVYPGLSFDITLLSPR
                     SFVVHVVDNVKQPGTYTSRALDRVSTVIARAGIATGSRRRIAIKHHDGTTGRADLVRY
                     ELTGDVAYNPFLLDGDVITVPFAYPVVTITGAVRRPGPYELVATKDLAELLDLAGGFT
                     SSVSRALPVRLVRRNARQQQATIELPFAGDAAPNTPLQDDDLITVRGSQELERSVLVI
                     GTEVGTIGMGGVGGADGTRAEPPDPATTSRRVSFVDGDTVLSLFNRIGGIKAPGDLRR
                     SYIARPRPGQPPEVIPVDLQALLVRREFGADRPVQPDDTIVVPPMQYSVLVEGAIARA
                     GLYNYDPAFGVPEYIAHAGGRTRTARDLDEAQLIDASGHTHPFRPGMKPAPGDAILVP
                     ERNFSRSEIAQLIIAGASLVVSGIAVTIAATR"
BASE COUNT          370 a          788 c          811 g          361 t
ORIGIN      
        1 gttgtcgggg tagacgatgt tcgcgaccgc cagtggcgcg ggcgccgcag cgtcgctctg
       61 gggcggtacg gacgcgtccg gtacttccag cgccgaccgt tgcaacgaga ccgatcctga
      121 cccgccgccg ccacaggcaa cacacacaag cgccacgacc gagatccgca tcgcttgtgt
      181 ggttcgacgg gtcgtgcgac cggtcgagac gaacgtggcg acggggcccg ggagctcgcc
      241 ggccttcttg gcagtcatca ccttggatcg cattcaaaac tccgggggaa tctgtgtcgg
      301 tttcgactct atggtcccgc tgaatctact gttccggccg cgccgcgtac agatcatccc
      361 gctcgcaact gcgagcgggc ttgaggtggt gcggaattaa cgattccgtg ccactgccgc
      421 gatttgccac ctcgctgacc gcgacccact tcgccatgat cagcgcggct cgttgctgcg
      481 ccagtacaac ttcacaccga tcgcgagctt accgcgtcgc ggcgatggtg accgcgatcc
      541 cgctgaccac gaggctggcg ccggcgatga tcagctgcgc gatctcggag cggctgaagt
      601 tgcgctcagg cacgaggatc gcgtcgcccg gcgcgggctt catcccggga cgaaacgggt
      661 gggtgtgtcc gctggcgtcg atcagctggg cctcatcgag atcgcgcgcc gtgcgcgttc
      721 ggccccccgc gtgggcgatg tactcgggca ccccgaacgc cgggtcgtag ttgtagagcc
      781 cggcccgtgc gatcgcgccc tcgaccagca cgctgtactg catcggcggc acgacgatcg
      841 tgtcgtccgg ctggacgggc cgatcggcgc cgaactcgcg gcggacaagc agggcctgga
      901 gatcgacggg gatcacctcg ggcggctggc cgggtcgcgg ccgtgcgatg tacgagcggc
      961 ggagatcgcc gggcgccttg atcccgccga tccggttgaa caacgacagc acggtgtcgc
     1021 cgtccacgaa cgagacacgg cggctcgtcg tcgcaggatc ggggggctcc gcgcgcgtac
     1081 catccgcgcc acccacgcca cccatgccga tcgtgccgac ctccgtaccg atgaccagca
     1141 cgctccgctc gagctcctgg ctgccgcgca cggtgatcag atcgtcatcc tgcagcggcg
     1201 tgttcggcgc ggcatcgccc gcgaacggca gctcgatcgt cgcttgctgc tgccgcgcgt
     1261 tgcgacggac cagccgcacc ggcaacgcgc ggctgacgct gctcgtgaat ccgccggcca
     1321 gatcgaggag ctcggcgaga tccttggtcg cgaccagctc gtaggggccg ggccggcgaa
     1381 cggcgccggt gatggtcacg accgggtagg cgaacggcac ggtgatcacg tcaccgtcga
     1441 gcaggaacgg gttgtaggcg acatcgccgg tgagctcgta gcgcacgagg tcggccctac
     1501 cggtggtgcc gtcgtggtgc ttgatcgcga tccgccggcg cgagccggtc gcgatgccgg
     1561 ccctggcgat cacggtcgac acgcgatcga gggcacggct ggtataggtg cccggctgct
     1621 tgacgttgtc gacgacgtgg acgacgaacg agcgcggcga cagcagcgtg atatcgaagc
     1681 tgaggcccgg gtagaccgcg cggatcttcg atttcatggc ggtccggacc acggacaggg
     1741 tcttgccggc aacggggatg aacccgaccc tcgggatgaa cgcgcggccc tcgaggtccg
     1801 tcgtgagctt gagctggagg ttctgcgagc cccagaagtc gagctcgaac acgtcgccgg
     1861 ggccgcacac gtaggtctcg ggatcgatcg gatgctcgat cgacagcggc ggctcggacg
     1921 ggaccaaccc cagtggcgat acgggcgcga cgatgtcgcc gccgagaacc tccccgccgg
     1981 gccgcagggt gatcgggacc ttcggaccta cgcccgtcga tctgggatca cccaatctgg
     2041 gatcaccgtc caatgtcctg ggatcggacg gcagtggctg cgccgacgcg atgcccgtga
     2101 tcgcgacgag cgcgatagcg gttctgcgca ctcctggcat cgcgggcacg ctaccgcagg
     2161 cgtcgcagcg acgctactgc gccccacgta gaagcgccac gacgaccacg gtctgccaca
     2221 ggatccgcgt cccggggctg cgacggcatg gatcgccctg gacgcgatgc gataccgtag
     2281 cgcatcccac gaccgcgacg acacctggcc cgcggcctgg ccgcggtgat
//
LOCUS       DASVOR010000691         4655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_386334,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000691 DASVOR010000000
VERSION     DASVOR010000691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386334"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..650)
                     /locus_tag="VF516_13665"
     CDS             complement(<1..650)
                     /locus_tag="VF516_13665"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8108774.1"
                     /translation="MRELLVRNADFRRLFLASVVSLAGDWFSFIAVAGLVTELTGRAG
                     APAFVFAATVLPVFLASPVAGALADRFDRRRILVIADLARVPIALALCAAAHWRSTPL
                     AFAAVTALAVGASFSDPIASAATPNLVDGADLASAQSLMGAVWGSMLMVGAGIGGLVA
                     ELFGRQAAFVVDAASFLASAWLIAGIRRPMQQAVGRRDAGSMRDALGYIRTTPVVLR"
     gene            complement(758..1468)
                     /locus_tag="VF516_13670"
     CDS             complement(758..1468)
                     /locus_tag="VF516_13670"
                     /inference="COORDINATES: protein motif:HMM:NF037747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HEX8108775.1"
                     /translation="MGRKEDVRQSTGRKADSNKPEFLAWDQFSDKKPQEALQAIYQHA
                     EDFSERCREWYWQSIRGKRLVSTGARFTAFTLGALGVIAPLAAAAIGQPGAQQSLSLL
                     AVPALAAAGLTQLADRVFGWSSGWLRYMSTVTAMESLTRQFQLDWAGYFVTLGQAQPV
                     QVKPLFDIAQRFEVQIADLQKRETDAWVIEFNTGLSALNDIVKNAHESSQKSAAEIRD
                     ALQQLNKASAVDRPERPN"
     gene            complement(1862..3298)
                     /locus_tag="VF516_13675"
     CDS             complement(1862..3298)
                     /locus_tag="VF516_13675"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108776.1"
                     /translation="MTLRPVWICLLLFGCGSKQPAPANPSPPNPDDEPAQTAAAAPTP
                     PPAPDPAPAPTPPPAADKPLFERLGGLPAITAVVEEFVGRTTTDPRIKQRFFNTDATQ
                     LKKLLVEFVASASGGGVKYPGRDMASSHAGMDLVDEEFGALVEDLVAALNKFNVPERE
                     KGELLGALGPLKPQIVVPADKLHPIPDAKLAKVSAMIPSVKNPEAAELLAAAVTAGKR
                     GQRSYAEQLFSRAELVAGPAALASASEVFREGAPPRIHTALKASPNDKPQLVPAVGKS
                     DEDSPAKRKEAGALHGTLTVEGKPLSSLGLVMLWPKGGKAVKRPAKFRVIEQRNKTFA
                     PHLMAVPVGSTVAFPNFDSIYHNVFSLSAPQRFDLGLYKSGDMRQVVFDKPGIIRLGC
                     NIHANMSAYLVVVDAPHYVVSEQDGSFSFRSLRPGKYKVQAWSEQSAEPMISEITVKA
                     GDNPLTLDLKGGASQGPSEDKFGASRAP"
     gene            complement(3317..>4655)
                     /locus_tag="VF516_13680"
     CDS             complement(3317..>4655)
                     /locus_tag="VF516_13680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108777.1"
                     /translation="IDRRSDVFSIGILLYELSTQSKLFRGTSDFALLQQIVENPIPLP
                     SARVPNYPRELQQIVMKALAKNPAERYPTAQALQLDLEEFAREHKLAMSSVRIARLMS
                     ALFEKRNDAWIRAQRAHSDHFIEVGATPAPAGHHTPTPGFLAVGSAPSTPSSENTPLP
                     TAAMLAEKIGTGQSGLGPAASPARRSPVLWLVGAILAAAVAVAVTVVDHTMSTAGDKA
                     MSAALSADVEKLAATFDAAARSAHMRADGIATTPMLRQAIETDAATLSDMANTEMVFT
                     ADRGEALEVFQFRGDRSTSLLRIPKTALSVQPLKGRATRFQTDGKGVTLAASAPISAY
                     RSGVAGGLVISAPVDLTAIRRTFGDHATYASLTGLGADVQLAGTPDNRGNAPVKLLVP
                     SSGDWNAGNAAVIASPKPAPGLIWAGHARYMSGGLAALLLAGFVVQLVRRSRS"
BASE COUNT          658 a         1619 c         1703 g          675 t
ORIGIN      
        1 cggagcacga ccggcgtggt gcggatgtag cccagcgcgt cgcgcatcga gccggcgtcg
       61 cgccgcccga ccgcctgctg catcggccgc cggatgccgg cgatcagcca cgccgacgcc
      121 aggaacgacg ccgcatcgac cacgaacgcg gcctggcggc cgaacagctc ggcgaccagg
      181 ccgccgatcc cggcgccgac catcagcatc gagccccaca ccgcgcccat cagcgactgc
      241 gccgacgcga ggtccgcgcc gtcgaccagg ttcggggtcg cggcgctcgc gatcggatcc
      301 gagaagctgg cgccgaccgc gagcgcggtg accgcggcga acgccagcgg cgtgctgcgc
      361 cagtgcgcgg ccgcgcacag cgccagcgcg atcggcacgc gcgcgaggtc ggcgatcacc
      421 aggatgcgcc ggcgatcgaa gcgatccgcg agcgcgccgg cgaccggcga cgcgaggaac
      481 accggcagca cggtcgccgc gaacacgaac gccggggcac cggcccggcc ggtcagctcg
      541 gtgaccagcc ccgcgaccgc gatgaacgag aaccagtcgc cggccagcga caccacgctg
      601 gccaggaaca gccggcggaa gtcggcgttg cgcaccagga gctcgcgcat cgcgcgcacc
      661 gctgcaagct agaccatcgc cgcgctcgcc gccacgccgc tgccaatcca ccagcggcag
      721 ggcgatcgct tggccgtgct gctcgtcgcg gccgcggtca gttcggtcgc tccggtcggt
      781 ccacggcgga cgccttgttc agctgctgca gtgcatcgcg gatctcggcc gcagacttct
      841 gggagctctc atgcgcgttc ttcacgatgt cgttgagcgc ggacaggccg gtgttgaact
      901 cgatgaccca cgcatcggtc tcgcgcttct gaagatctgc gatctggacc tcgaagcgct
      961 gggcgatgtc gaacagcggc ttgacctgga ccggctgggc ctgccccagc gtgacgaagt
     1021 atcccgccca gtcgagctgg aactgccggg tcaggctctc catcgcggtc accgtcgaca
     1081 tgtagcgcag ccatccgctc gaccagccga acactcgatc ggcgagctgc gtgagcccgg
     1141 cggccgcgag cgccggcacg gcgagcagac tcagcgattg ctgggcgcct ggctgcccga
     1201 tcgccgcggc ggcgagcggc gcgatcacgc ccagcgcgcc gagggtgaac gcggtgaagc
     1261 gcgcaccggt ggagaccagg cgcttgccgc gaatgctttg ccagtaccac tcccggcacc
     1321 gctccgagaa gtcctcggcg tgctgataga tcgcctgcaa cgcctcctgc ggcttcttgt
     1381 cgctgaactg atcccaggcg aggaactccg gcttgttgct atcggccttg cgtcccgttg
     1441 attgcctgac gtcttccttg cgtcccatcg atccctccac gcggcctcac aggttcaccg
     1501 cggcgcctac acggccccgc ggtgccgccc cccaggcggc acgattcacg aagacacctt
     1561 cccccacacg cggcccatgg tactcggctc tgtccttcgc gtccactggc gaacgcagtt
     1621 gctacccgac gattaacccg gattgccggt ccggagcgcc ggaagtcagc tggcaggggg
     1681 ggccggcgct cggtgggtgg gcggcgagag gcccacagcg agcggaggaa cgcgacgagg
     1741 tcggagtgct cctgtcgccg gcgcaggccg aaacggcgaa ggccgcggcg ctccatcgca
     1801 ggagcggctc gcggcctgga gatcaggggg ctcgccggcg gagccgggcg ggcggggaac
     1861 gctacggcgc gcgcgaggcg ccgaacttgt cctcgctcgg gccctgcgac gcgccgccct
     1921 tgaggtcgag cgtcagcggg ttgtcgccgg ccttgacggt gatctcgctg atcatcggct
     1981 ccgcgctctg ctcgctccac gcctgcacct tgtacttgcc ggggcgcagg ctgcggaacg
     2041 agaagctgcc gtcctgctcg gacacgacgt agtgcggagc gtcgacgacg acgaggtagg
     2101 ccgacatgtt cgcgtggatg ttgcagccga gccggatgat gccgggcttg tcgaacacga
     2161 cctggcgcat gtcgccgctc ttgtacagcc cgaggtcgaa gcgctgcggc gccgagagcg
     2221 agaacacgtt gtggtagatc gagtcgaagt tcgggaacgc gacggtcgag ccgaccggga
     2281 ccgccatcag gtgcggcgcg aacgtcttgt tgcgctgctc gatcacgcgg aacttcgccg
     2341 ggcgcttgac ggccttgccg cccttgggcc acagcatcac cagcccgagg ctggacagcg
     2401 gcttgccctc caccgtcagc gtgccgtgca gcgcgccggc ttccttccgc ttggccgggc
     2461 tatcctcgtc cgacttgccg accgcgggga ccagctgcgg cttgtcgttc ggcgacgcct
     2521 tgagcgcggt gtggatccgg ggcggggcgc cctcgcggaa cacctcggac gcgctggcca
     2581 gggccgccgg gccggcgacc agctcggcgc ggctgaacag ctgctcggcg taggaccgct
     2641 gcccgcgctt gccggcggtg accgcggcgg cgagcagctc ggccgcctcc gggttcttga
     2701 ccgacgggat catcgcgctg accttggcga gcttggcgtc cgggatcgga tggagcttgt
     2761 ccgcggggac gacgatctgc ggcttgagcg ggccgagcgc tccgagcagc tcgcccttct
     2821 cgcgctccgg gacgttgaac ttgttgagcg cggcgaccag gtcctcgacc agcgcgccga
     2881 actcctcgtc gacgagatcc atgccggcgt gggagctcgc catgtcgcgc ccggggtact
     2941 tgacgccgcc gccgctggcc gaggccacga actcgaccag gagcttcttg agctgggtcg
     3001 cgtcggtgtt gaagaaccgc tgcttgatcc gcgggtcggt ggtggtgcga ccgacgaact
     3061 cctcgaccac ggcggtgatc gcgggcaggc cgcccagccg ctcgaacagc ggcttgtcgg
     3121 cggcgggtgg aggtgtcggc gccggcgccg ggtccggggc gggtggaggt gtcggcgccg
     3181 ccgccgccgt ttgcgccggc tcgtcgtcgg ggttgggcgg cgatgggttc gcgggcgcgg
     3241 gctgcttcga gccgcacccg aagagcagca agcagatcca cacgggtctg agcgtcacgg
     3301 gatctcctgt cgaggattag gaacgcgagc ggcgtaccag ttggacgaca aagcccgcca
     3361 gcagcagcgc cgcgagccct ccggacatgt aacgagcgtg tccggcccag atcaagccgg
     3421 gggccggctt tggtgaggcg atcaccgcgg cgttgcccgc gttccagtct ccgctcgacg
     3481 gcacgaggag cttgaccggc gcgttgccgc ggttgtcggg cgtcccggcg agctggacgt
     3541 cggcgcccag gccggtgagc gaggcgtagg tcgcgtggtc gccgaacgtg cggcggatcg
     3601 cggtcagatc gaccggcgcc gagatcacca ggccgcccgc gacgccggag cggtacgccg
     3661 agatcggcgc gctcgccgcc agggtcacac ccttgccgtc ggtctggaac cgggtggccc
     3721 ggcccttgag cggctgcacc gagagcgcgg tcttggggat ccgcaacagc gacgtcgagc
     3781 ggtcgccgcg gaactggaac acctccagcg cctcaccgcg gtcggcggtg aacaccatct
     3841 cggtgttggc catgtcgctg agcgtcgcgg cgtcggtctc gatcgcctgg cgcagcatcg
     3901 gggtcgtggc gatcccgtcg gcgcgcatgt gggcggaccg cgccgcggcg tcgaacgtgg
     3961 ccgcgagctt ctcgacatcg gccgacagcg cggcgctcat cgccttgtcg cccgcggtgc
     4021 tcatcgtgtg gtcgacgacg gtgaccgcga ccgcgaccgc ggccgcgagg atcgcgccga
     4081 cgagccacag caccggcgaa cggcgcgccg gcgaggccgc cgggccgagc ccgctctgcc
     4141 cggtgccgat cttctcggcg agcatggcgg cggtcggcag cggcgtgttc tcggacgatg
     4201 gcgtcgacgg cgccgagccg accgcgagga accccggggt cggcgtgtga tggccggccg
     4261 gggccggcgt ggcgccgacc tcgatgaagt ggtcgctgtg cgcgcgctgg gcgcggatcc
     4321 aggcgtcgtt gcgcttctcg aacagcgccg acatcagcct cgcgatccgg accgacgaca
     4381 tcgcgagctt gtgctcgcgg gcgaactcct cgaggtcgag ctggagcgcc tgcgccgtgg
     4441 ggtagcgctc ggcggggttc ttcgcgagcg ccttcatcac gatctgctgg agctcgcgcg
     4501 ggtagttggg gacgcgcgcc gacggcagcg ggatcgggtt ctcgacgatc tgctggagca
     4561 gcgcgaagtc gctggtgccg cggaacagct tggactgggt cgacagctcg tagagcagga
     4621 tcccgatcga gaacacgtcg ctgcgccggt cgatc
//
LOCUS       DASVOR010000692         3411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_182247,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000692 DASVOR010000000
VERSION     DASVOR010000692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3411
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182247"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF516_13685"
     CDS             complement(<1..276)
                     /locus_tag="VF516_13685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108778.1"
                     /translation="MATRQASLALRDAMTAAERAAASAVICDAANATLAARLSPGQVV
                     ALYAAKGSEVNTERIDAFARGRGLVVAYPRVNGRARLLAFHAVLPEAR"
     gene            complement(410..730)
                     /locus_tag="VF516_13690"
     CDS             complement(410..730)
                     /locus_tag="VF516_13690"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8108779.1"
                     /translation="MDKREAVRVPVRVPALCRSEGVVIDGTVEDLSRTGLFLRASEVM
                     AEGSSAEIHLNLPGEEPLQLSAEVVRVENTEERAGMGLRIVHDAQDCRALANFIMRQH
                     ATLR"
     gene            946..1017
                     /locus_tag="VF516_13695"
     tRNA            946..1017
                     /locus_tag="VF516_13695"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:978..980,aa:Lys,seq:ttt)
     gene            1034..1106
                     /locus_tag="VF516_13700"
     tRNA            1034..1106
                     /locus_tag="VF516_13700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1068..1070,aa:Glu,seq:ttc)
     gene            1348..1629
                     /locus_tag="VF516_13705"
     CDS             1348..1629
                     /locus_tag="VF516_13705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108780.1"
                     /translation="MSAAVAALAGLDAQPERLQVGLERVHGVGGVGGVDRGHVAGGHH
                     EVDGATHPLAKSLRRPRVVIWNPTPRNFQSAPSVQRMSPRSRRISSASL"
     gene            1726..2460
                     /locus_tag="VF516_13710"
     CDS             1726..2460
                     /locus_tag="VF516_13710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108781.1"
                     /translation="MKYGGFAFCFHIGEDQSRELLDRDVVLLFVGEHAEAHPAEGLSS
                     RVPTQARVVQSPSLQATTSMARREPGPSWSPSSPPPRPAVLLRGRQHRRAGRGSRRPQ
                     RAARAPGTVPGNLARPPAQPGTRATAASTAALAKRIAENQRSGVRLAAMAKVATSAAK
                     RSKLKKTERCRKRPAGKGVFQTAAMVGGIVSGVRSVKELAYWVKDLIAKPKPKRRGKS
                     GVAKPKKPTKKFTARATKKPVRGRAH"
     gene            complement(3084..>3411)
                     /locus_tag="VF516_13715"
     CDS             complement(3084..>3411)
                     /locus_tag="VF516_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108782.1"
                     /translation="APEQDGLGARPGWSLRELVRRKLGDAARVTALVSQDGKRVGLDA
                     AAWGDAGATPILRVNHQGQLRFHWVGGKGRKAGLKDVRTIEVVVPAAASGGQLGGQNR
                     SSLIEL"
BASE COUNT          542 a         1236 c         1096 g          537 t
ORIGIN      
        1 ccgcgcctcc ggcagcaccg catgaaacgc cagcagccgc gctcgcccgt tcacccgcgg
       61 ataggccacc accagcccgc gcccgcgcgc gaacgcgtcg atccgctccg tgttgacctc
      121 gctgcccttc gccgcgtaca gcgccaccac ctggcccggc gacagtcgcg ccgcgagcgt
      181 cgcgtttgcc gcatcacaga tcaccgcgct cgccgccgcg cgctccgccg ccgtcatcgc
      241 atcgcgcaac gcgagcgacg cctgccgggt ggccaatttg ccacgcgggc ctcgagggtc
      301 tgggggcagc acgggcggga gcaagtgact aggcggcgat catagccaca acgcgcggca
      361 gcctatcaag cccccaagcc atcgaatcgg ctaggaaaac caatacgact cagcgcagcg
      421 tcgcgtgctg gcgcatgatg aagttcgcca gcgcccggca gtcctgcgcg tcgtgcacga
      481 tccgcagccc catccccgca cgctcctccg tgttctccac gcgcacgacc tcggcgctca
      541 gctgaagcgg ctcctccccc ggtaagttca ggtggatctc ggccgacgat ccctccgcca
      601 tgacctcgga agcccgcaaa aacaggccgg tacgcgacag gtcctcgacc gtgccgtcga
      661 tcaccactcc ctccgaccgg cagagcgccg ggacccggac ggggactcga actgcttccc
      721 gcttgtccat gtgccaaaag gtacggtagc gactcacgct ggcgagagca aactttctcc
      781 ggtccgcctc acggccatag ctggctgaaa atgctcgggg aatcggccat cacagcctga
      841 gcaacgctcg cgcgcaaggc tttggcctcg gcgctgcccg cggccgcgac cgcccgccgg
      901 cttgcattct cgccgcagat catccagatt gcgcggcacg ccagagggcc catagctcag
      961 tggtagagca ccggcctttt aagccgaggg tcgaaggttc gagaccttct gggctcaccc
     1021 cctaactccg caagacccca tcgtccagag gcctaggaca cccgcctttc acgcaggtaa
     1081 cacgggttcg aatcccgttg gggtcgcttg ttttatccaa cgtttctgag cgcttagctc
     1141 ggtggtagca gagctggtag caagctccag gacctcaccg ccgagcaggc ccacgcggag
     1201 cttgctcacc tcgcggcaga tcgtcgccca tgggaaccgc tcgtaacccg agttcacgtc
     1261 atcggacaca gtgtgtgtag tgcaccttac gatcagcgag tcggccccgt cttcgatggt
     1321 ccgggttttg taccaggccc gcaggtcatg tccgccgcgg tcgcggcgct cgccggcctc
     1381 gacgcgcagc ccgagcgcct gcaggtcggc cttgaacgcg tgcacggcgt cggcggcgtc
     1441 ggcggcgtcg accggggtca tgttgcgggt gggcaccacg aggtcgatgg cgccacccat
     1501 cccttggcga agtcgcttcg gcggcctcgt gtcgtgatct ggaacccgac gccgcggaat
     1561 ttccagagcg cgccctcagt ccagcggatg agcccgcggt cgagaaggat ctccagcgcc
     1621 tcgttgtagc gctcccagcg gcgaaactgc ttcggctcgg tcgaagggtc tggagtgtgt
     1681 tggcccttgt agccggtgaa cggcgccaga ggatcgttgg tcacattgaa atacggcggc
     1741 ttcgccttct gttttcatat cggcgaagat cagtcgcgcg agctgctcga tcgcgatgtt
     1801 gtccttctct tcgtcggtga gcatgctgag gcccatcccg ccgagggtct ctcgtcgaga
     1861 gtgccgacgc aagctcgcgt ggtgcagtcg ccatcgctgc aggccaccac cagcatggcg
     1921 cgccgcgagc cggggccgag ctggtctcca tcgtcgccgc ctccgcgacc agccgtgctc
     1981 ctccggggcc gacagcaccg gcgagctggt cgaggtagcc gccgccctca gcgagctgcc
     2041 cgagctcccg gtactgtccc cggaaacctc gcccgcccgc cggcgcagcc cggcacccga
     2101 gccaccgctg ccagcaccgc cgcgctggcc aagcggatcg ccgaaaacca gcgcagcggt
     2161 gtacgactcg ccgcgatggc gaaggtagcg acgagcgccg caaagaggtc gaagttgaag
     2221 aagaccgagc gatgcagaaa gcggcccgcc ggcaagggtg tgttccagac cgccgcgatg
     2281 gtcggtggca tcgtcagcgg cgtgcgcagc gtgaaggagc ttgcttactg ggtgaaggac
     2341 ctgatcgcga agccgaagcc gaaacggcgc ggcaagtccg gcgtggcgaa gccgaagaag
     2401 ccgaccaaga agttcaccgc gagggcgacc aagaaacccg tgcgcggccg ggcgcactag
     2461 ccgggctcct cttaaacctc ggtgacctcc cggctgcgcg cacgatgttc gagcaggcgc
     2521 ttccgttggt ccgcacgacc tacagcgacg atcacccgct cgtcgcggcc atccgttcaa
     2581 agctttctga actgcgtcgg cgaatgagtt gagcgatgtc gtcctaggtc agattccagt
     2641 tcgccgccgg cggcgacccg tgacaacagg aagtctccgc cgccgagatc gccctcgcca
     2701 agcgaggcgc gctaccgccc gccgcgcttc gtgggcgcgc tcggtccctc gatctggtcg
     2761 agcgcccggg tcagcagcga gcgcaccgga tcggcgcggc tgatcttgcg actctgcgta
     2821 acggcgggtc cccgccgtta cagcccggct cgcgatccgc gctcgcgagc gccgcggtca
     2881 tgccgccatg ctcggatcag gcgcgccgct caatccggag cacccaggaa cttcgcggag
     2941 cgctggctgg ttccgctcgt ccgccatgcg gagccggggt cgctcgtcga tcgcgtatcc
     3001 tgggatcgcg tgcatcgcgt cctcgctatt cgcgtcgtgc gctgaagtag ccgatgcctc
     3061 cgtcggccac ctcttccggc aacctacagc tcgatcaggg acgaccggtt ctgaccgccg
     3121 agttggccgc cgctcgcggc agccggcacg acgacctcga tcgtccggac gtccttcagg
     3181 cccgccttgc ggcccttgcc gccgacccag tggaaccgga gctggccctg gtggttgacg
     3241 cgcaggatcg gcgtggcgcc ggcgtcgccc caggccgccg cgtcgagccc gacgcgcttg
     3301 ccatcctggc tgaccagggc cgtgacccgc gcggcgtctc ccagcttgcg gcggaccagc
     3361 tcgcgcagcg accagccggg ccgcgcgccg aggccgtcct gctccggcgc c
//
LOCUS       DASVOR010000693         1578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211249,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000693 DASVOR010000000
VERSION     DASVOR010000693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211249"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF516_13720"
     CDS             complement(<1..375)
                     /locus_tag="VF516_13720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108783.1"
                     /translation="MRYEDAVESLFRAPHEQFVTERRRLAGELKAAGDKAGAGKLAKL
                     ARPPISAWIVDQLWWRSRDLFEALFATAEQLRRGELGAAAAHRDAIARLRAQAAQLLG
                     EAGHAATEATLRRVAQTLSALAA"
     gene            complement(459..707)
                     /locus_tag="VF516_13725"
     CDS             complement(459..707)
                     /locus_tag="VF516_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108784.1"
                     /translation="MPRTNSDWDIVDEASLESFPASDPPAWGSYHAVASEATVAIPDA
                     NGIVVRRSPLRIALWLIAATAALGGLILLGAKLRRRWR"
     gene            complement(738..1025)
                     /locus_tag="VF516_13730"
     CDS             complement(738..1025)
                     /locus_tag="VF516_13730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108785.1"
                     /translation="MRSIRFQENPPDGTWVALSADMQPSYLSEQAPGLEGPDYVALVI
                     EWNDEEDDAAALVTSSEPAYPRGTIRTIAAVVGALGALLLAAWGIRRLRSA"
     gene            1037..1360
                     /locus_tag="VF516_13735"
     CDS             1037..1360
                     /locus_tag="VF516_13735"
                     /inference="COORDINATES: protein motif:HMM:NF036497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8108786.1"
                     /translation="MSQRERRIPWIAVGVAAVGIAIAAWRIDRLRSAPLALASRHVSA
                     SDLVKLRPGSSCAALGLEDGDVIRAINGTPLVSADQVLELVARAPRRSRSISCAAAGQ
                     CFSTT"
BASE COUNT          222 a          538 c          586 g          232 t
ORIGIN      
        1 cgcggcgagc gccgacaggg tctgggccac ccggcgcagc gtggcctcgg tcgcagcgtg
       61 gccggcctca cccaggagct gtgcggcctg ggcgcgcagc ctggcgatcg cgtcgcggtg
      121 tgcggcggcg gcaccgagct cgccgcggcg gagctgctcg gcggtggcaa acaatgcctc
      181 gaacaggtcg cgggagcgcc accacagctg gtcgacgatc cacgccgaga tcggcggccg
      241 cgccagcttg gcgagcttgc cagcgcctgc cttgtcgccg gccgccttga gctcgccggc
      301 gagccgtctg cgctcggtca cgaactgctc gtggggagcc cggaacagcg actcgacagc
      361 gtcctcgtag cgcatcactc acatcgtatc agcgcacagc ggctcacgct gccccgccag
      421 cgcacggtgc gcgtagcgcc gtgcgtgcat gccccggctc agcgccagcg gcggcggagc
      481 ttggcgccga gcaagatcag gccgcccagc gcggcggtcg ccgcgatcag ccacagcgcg
      541 atccgcaggg ggctgcgccg gacgacgatc ccgttggcgt cggggatcgc gacggtcgcc
      601 tcggacgcca cggcgtgata cgagccccac gccggcgggt cactcgccgg gaacgactcg
      661 aggctcgctt catcgacgat gtcccagtcg gagttcgtgc ggggcatggt ggccgtggtt
      721 tgcactcgac gtgccgatca ggccgagcgc aggcggcgga tgccccaggc ggcaaggagc
      781 agtgcgccca gtgcgcccac gaccgcagcg atcgtgcgaa tcgtgccgcg ggggtaggcg
      841 ggttcggagc tggtgaccag cgcggcggcg tcatcctcct cgtcattcca ttcgatgacc
      901 agggccacgt agtccgggcc ctcgagtccg ggggcttgtt cgctgaggta ggagggctgc
      961 atgtccgctg agagagcaac ccacgtgccg tccggcgggt tttcctggaa ccggatcgag
     1021 cgcatgctct ccggagatgt cgcagcgcga gcggcggatt ccgtggatcg cggtcggggt
     1081 cgcggcggtc gggatcgcga tcgccgcgtg gcgcatcgat cggctgcgat cggcccctct
     1141 cgcgctggcg agccggcatg tctcggcaag cgatctggtg aagctccggc cgggctcctc
     1201 ctgcgcggcg ctcggcctgg aggacggcga cgtgatccgc gcgatcaatg gcacgccgct
     1261 ggtgtccgcc gatcaggtcc tggagctggt ggccagggcc ccacgcaggt cgcgctcgat
     1321 ctcgtgcgcc gcggccggcc aatgcttctc aactacttga tctattaggg gatcggcccc
     1381 gtgccggttc gggctggacg cactgtcaga ccgagctgat actgaacata tgttcccatg
     1441 agcgacgacc tcggagacct gttttcgaag gcgaagggcg tggccgccac cgcccgcggc
     1501 acctggatgc agaaggggct ccagaaggcg agccgggacc cggtcgagcc tgccggggcg
     1561 cccggcgacg agagcggc
//
LOCUS       DASVOR010000694         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_503434,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000694 DASVOR010000000
VERSION     DASVOR010000694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503434"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2183
                     /locus_tag="VF516_13740"
     CDS             <1..>2183
                     /locus_tag="VF516_13740"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8108787.1"
                     /translation="FTLASPAEVQRFVAILRELRAEPSCYLVLALRADFYGTFMDSAL
                     WPEAPGMSRLDVVPLRGAALAQAIRDPARAVRVYLEAPLCDRLVADAAEEPGVLPLVQ
                     ETLRLLWDRRRERFLGLDAYEALGRGGRALDVAIARRAATAMAGLGATEQTIARRVLL
                     RLVSFGEGRPDTRRQQEVAALRSADDDDEDFSRVLRQLVEHRLITVGEAEPDHGALAD
                     LSHEALITGWPELQMWIARRRADEQRRRRLEAKVGEWIEHGRGTASLLDAIELAEARA
                     WLQSDAARELGFGPELPELVQASGAELARLERQRRRRTARAIALLVGFTLVASSLAVM
                     AWQQRRDAQRSGALAQQRLGMRYLEAGRAYVVADHPMQALPFLIEARAQGIDGRILEL
                     LFAQAASNLPLVTLAGHGSFVTGAAFSPEGARLLTISNEQVRLWDSATGKALAPPLVH
                     RGPVQMAVFSPDGTRLATASYDLTAQVWDAATGKQVTPPLVHQGLVHGVAFSADGASL
                     VTAGGDLTARVWDATTGALRLTLPHAAPVTAVAFSPDGSRVVTASEDRVARIWDAATG
                     TQLIARLAHDGVVTAAAFSRNGAYIATASEDMTARVWDATTGAPITAPLVHDASVIAA
                     GFDRDGARLVTASGDRAQVWDAASGTPVGEPLVHTGLVTSARFSPDGTWVVTASADHT
                     ARVWDAATGQPLVVLEHQDAIADAAFSPDGTRVVTASEDGTARVW"
BASE COUNT          301 a          754 c          830 g          298 t
ORIGIN      
        1 gttcacgctg gccagccccg ccgaggtcca gcggttcgtc gcgatactgc gcgagctgcg
       61 cgccgagccg tcatgctacc tggtactcgc cctgcgcgcg gacttctacg ggacattcat
      121 ggacagcgcg ctgtggcccg aggcgcccgg catgtcgcgc ctcgatgtgg tgccgctgcg
      181 tggcgcggcg ctcgcacagg cgatccgcga tccggcccgc gcggtgcgcg tgtacctcga
      241 ggcgccgcta tgcgatcgtc tggtcgcgga cgccgccgag gagcccggtg tgcttcccct
      301 ggtgcaggag acgttgcggc tgctgtggga ccgacgccgc gagcgcttcc tcggactcga
      361 cgcctacgag gcgctcggcc gcggaggccg cgcgctcgat gtcgccatcg cgcggcgggc
      421 ggcgaccgcg atggcgggac tcggcgcaac cgagcagacc atcgcgcggc gcgtgctgct
      481 ccggctggtg agcttcggcg agggtcggcc cgacacccgc cgccagcagg aggtggcggc
      541 gctgcggtcg gccgacgacg acgacgagga cttctcgcgc gtgctgcgcc agctggtcga
      601 gcaccggctg atcaccgtcg gcgaggccga gcccgatcac ggcgcgctgg cggacctgtc
      661 gcacgaggcg ctgatcacag gatggccgga gcttcagatg tggatcgcac ggcgtcgggc
      721 cgatgagcag cgccgccgcc ggctcgaggc caaggtcggc gagtggatcg agcatggtcg
      781 cggcacggcc agcctgctcg atgccatcga actggcggaa gcgcgggcat ggctgcagag
      841 cgatgccgcg cgcgaactcg gcttcgggcc agagctgccc gagttggtcc aggcaagcgg
      901 cgcggagctc gctcgcctcg agcgccagcg ccggcggcga accgcgcgcg ccatcgcgct
      961 gttggtcggc ttcaccctgg tggcatcgag cctggcggtc atggcgtggc agcagcgccg
     1021 cgacgcgcag cgcagtggcg ctctggccca gcagcggctg gggatgcggt acctcgaggc
     1081 cggacgggcg tacgtggtcg cagatcaccc gatgcaggca ctgccgttcc tgatcgaggc
     1141 cagggcccag gggatcgatg gccggatcct ggagctcctg ttcgcccaag cggcgagcaa
     1201 cctgccgctg gtcacgctgg caggccacgg ctcgttcgtc accggcgcgg cgttcagccc
     1261 tgaaggtgcg cggttgctca cgatcagcaa cgagcaggtg cggctgtggg actccgccac
     1321 gggcaaggcg ctggctccgc cactcgtgca tcgcggcccg gtgcagatgg ctgtgttcag
     1381 ccccgacggt acacggctgg ccacggcgag ctacgatctc accgcgcagg tgtgggacgc
     1441 ggcgaccggc aagcaagtga cgccgccact ggtgcatcag ggcctggtcc acggggtcgc
     1501 attcagcgcc gacggcgcgt cgctcgtgac cgccggagga gacctgacgg cgcgggtgtg
     1561 ggacgcgacg accggcgcgc tgcggctcac gctgccgcac gccgcgccgg tcacggccgt
     1621 cgcgttcagc cccgacggca gccgagtcgt caccgcgagc gaagatcgcg tcgcgcgtat
     1681 ctgggacgcg gcgaccggca cgcagctgat cgcgcggctc gcccacgatg gcgtcgtgac
     1741 cgcagcagcg ttcagccgca acggcgcgta catcgccacg gcgagtgagg acatgactgc
     1801 gcgggtgtgg gacgcgacga ccggcgcgcc gatcacggcg ccgctcgtcc acgacgcatc
     1861 ggtcatcgcc gccgggttcg atcgcgacgg tgcgcggctg gtcactgcga gcggtgatcg
     1921 cgcgcaggtg tgggacgcgg cgagcggtac gccggtcggg gagccactcg tccacaccgg
     1981 cctggtaaca tcggcgcggt tcagccccga cggtacgtgg gtcgtcaccg cgagcgctga
     2041 ccacaccgcg cgggtatggg acgcggcgac cggacaaccc ctcgttgtcc tcgagcatca
     2101 ggacgcgatc gccgacgccg cgttcagccc cgacggaacg cgcgtcgtca cggcgagtga
     2161 ggatgggacg gcgcgggtgt ggg
//
LOCUS       DASVOR010000695         5299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_182271,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000695 DASVOR010000000
VERSION     DASVOR010000695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182271"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            69..221
                     /locus_tag="VF516_13745"
     CDS             69..221
                     /locus_tag="VF516_13745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108788.1"
                     /translation="MSQKAPQDPDLRRAPTQKLKTQKRPNTDLPPDDDVEVPLDDPGK
                     PGAPMK"
     gene            295..1347
                     /locus_tag="VF516_13750"
     CDS             295..1347
                     /locus_tag="VF516_13750"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8108789.1"
                     /translation="MVEDPRATLSLLTQRTEESARLLQLAGTTVLVADATMIQIYDLL
                     KRLATSDLSVLVSGETGVGKEHVAHALHHWSRRAARPFVAINCASLPEALIESELFGH
                     ERGAFTSAVSSKPGHLEAASGGTVFFDEVGELPLAAQAKLLRVLESRRVLRIGSRKED
                     PIDVRVIAATNRDLEAEVKEGRFREDLFFRLGGAKIVIPPLRERPRELAILARCFLER
                     ACAAAGRVVPELSPATLAVLARHRWSGNVRELKNEMEYVAATVTELVIEPWHLSERII
                     ATSPSTTATARSPARAEGELRFRPIHDELRDLELRRMREALFAAGGVQKRAAELIRMP
                     LRTFAMKYKQYGLGDR"
     gene            complement(1363..2673)
                     /locus_tag="VF516_13755"
     CDS             complement(1363..2673)
                     /locus_tag="VF516_13755"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8108790.1"
                     /translation="MWRIALACLLASAPAAASPTIIKAAHLIDGVSDRPRDGVAVLVD
                     GDAIVALGTAAELTAKAPGAKLVDLGGATLLPGMIDAHTHVFLDDDVGPGVYDAILLK
                     HSAPYRAIEAVANARTALMYGFTALRDLETEGAMYADVDVKNAIAHGVVPGPRMQVAT
                     RAFAPTGMYPLRAGYNWELELPIGVQTIDGPDQARKAVREQVSFGADWIKFYADHGVY
                     EGRPDRPVRSIVNFTPAEAAAIVDEAHRLGVKVAAHANGWDGIDSALRAGVDSIEHGQ
                     GITDDLATRMIAQSVVWCPTMMAYQYAIKKLGADPRSWMAAHHKAALARAFKRGVRIA
                     FGTDAGAFPWSMNPAAEAKLMVDAGMPAMAVIRSVTSAAAALLDPLCRPGAKACAKSE
                     LGEIAAGKHADLIAVAGNPLQDITELERVTWVMKGGAVWKSPAP"
     gene            complement(2708..3313)
                     /locus_tag="VF516_13760"
     CDS             complement(2708..3313)
                     /locus_tag="VF516_13760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8108791.1"
                     /translation="MVEPAEREASERAIRALCEAGDHAAATTAALRGYGVELLGFLRA
                     LAGDHDLAAEAFAELGEDVWKGLPKFRWESSLRSWLYALARNALAQLRRDPRRRRERN
                     LPLSIAPEMAAVVRTITREIQRTEVKDEFRLLREQLDPEEHEILLLRLDRDMAWKDIA
                     RILGGDGDLDTRAAALRKRFERAKQRLKKLAVERGLIQRRK"
     gene            complement(3328..5013)
                     /locus_tag="VF516_13765"
     CDS             complement(3328..5013)
                     /locus_tag="VF516_13765"
                     /inference="COORDINATES: protein motif:HMM:NF006181.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8108792.1"
                     /translation="MGTRHLQFWPNVPHHLTPPETSLWFNAEVSATRFPGKTCLNFYD
                     SVVTFSAFRREAERLAGFLQQACGVKRGDRVALYLQNSPQFVIAYYAILRADAMVVPI
                     NPMNLTKEVGHIVQDSGATVLFAAQDGPSMPSLSRIQPLVGNGLSHVIVACYSDYLTT
                     PTDLDVPAWIKEPRQSQTGSGLTLWSDAMAAGATPGPHLAGPDDLCVMPYTSGTTGNP
                     KGCVHRHRSVMFTTVAGPQWNRTFQDETILSILPYFHATGMQGGMNAPIYAGVSIILL
                     PRWDRDVAGQLIQRYKVTGFTAVPTMIVDLLSNPRLASYDLSSLRQVGGGGAAMPEAV
                     AQKLQDLCGLEFIEGYGLSETIAPTHINPPQRPKKQCLGIPIFNTDARVVNPDTLVEL
                     PPGEVGEIISHGPQVFDGYWNNPQANAACFVELDGKRFFRTGDLARTDEDGYYFLVDR
                     LKRMINVAGFKVWPAEVESMLYGHPDIQEAVIIAGQDPRRGETVKAVVVLKPAAKGRV
                     TEADLVRWARDNMAAYKVPASFEFVETLPKSATGKILWRVLQEQQDARERAAT"
BASE COUNT          739 a         1849 c         1919 g          792 t
ORIGIN      
        1 cgagctccgc cggggctcgg ggcttgcaca tgggtgtcgg cgcatccacg tcaatccagg
       61 agatgaccat gtcgcagaaa gctccgcagg atccggacct ccggcgcgcc ccgacgcaga
      121 agctgaagac gcagaaacgg ccgaacaccg acctgccgcc cgacgacgac gtcgaggtcc
      181 cactcgacga tcccggcaag ccgggagcgc cgatgaagtg agcaggcgcc cgcggcggcc
      241 acgatgacac agcgagcgac ggtcggtgcc cgaccgatcc atggagaagg cgacatggtc
      301 gaggacccca gagcgacgct gagcttgctc acgcagcgga cggaggagag cgcgcgcctg
      361 ctccagctcg caggcacgac ggtgctcgtc gccgatgcca cgatgatcca gatctacgat
      421 ctgctcaagc gcctcgcgac cagcgatctc tcggtgctgg tctcgggcga gaccggcgtc
      481 ggaaaggagc acgtggcgca tgcgcttcat cactggtcgc gacgcgcagc ccggccgttc
      541 gtcgcgatca actgtgcgtc cctgcccgag gcgctgatcg agagcgagct gttcggccac
      601 gagcggggcg cgttcacgag cgccgtgtcg agcaagcccg ggcatctcga agcggccagc
      661 ggtggcacgg tgttcttcga cgaggtggga gagctgccgc tcgccgcgca agccaagctg
      721 ttgcgcgtgc tcgaatcgcg ccgcgtgctc cggatcgggt cgcgcaagga agatccgatc
      781 gatgtccgcg tcatcgcagc aaccaatcgc gacctcgagg cagaggtcaa ggaagggcgg
      841 tttcgcgagg atctgttctt ccgcctcggt ggcgccaaga tcgtcatccc gcccctgcgc
      901 gagcggccgc gcgagctggc gatcctggcg cggtgctttc tcgagcgcgc gtgcgcggcc
      961 gccggtcgcg tggttcccga gctgtcgcct gcgacgctcg cggtgctcgc gcgtcaccgc
     1021 tggtcgggca acgtccgcga gctcaagaac gagatggagt acgtcgccgc gaccgtcacc
     1081 gagctggtga tcgagccctg gcacctctcg gagcggatca tcgcgacgag cccgagcacg
     1141 accgcaacgg cgcggtcccc agcccgcgcg gagggcgagc tgcgattccg gccgatccac
     1201 gacgagctgc gcgatctcga gctccgccgc atgcgggaag cgctgttcgc cgccggcggc
     1261 gtgcagaagc gagcggcgga gctgatccgc atgccgctgc ggacgttcgc gatgaagtac
     1321 aagcagtacg gtctcggcga tcgctgaacg ccccggccgt gatcacggcg cgggcgactt
     1381 ccacaccgcg ccgcccttca tcacccaggt cacccgctcg agctccgtga tgtcctgcag
     1441 cgggttgccg gcgaccgcga tcaggtcggc gtgcttgccg gccgcgatct cgccgagctc
     1501 ggacttcgcg cacgccttcg cgccgggccg gcacagcgga tcgagcagcg ccgcggcggc
     1561 cgacgtcacc gagcggatca ccgccatcgc cggcatcccg gcgtcgacca tcagcttggc
     1621 ctcggccgcc gggttcatgc tccacgggaa cgccccggcg tcggtgccga acgcgatccg
     1681 cacgccgcgc ttgaacgccc gggcgagcgc ggccttgtgg tgcgccgcca tccagctccg
     1741 cgggtcggcg ccgagcttct tgatcgcgta ctggtacgcc atcatcgtcg ggcaccacac
     1801 cacgctctgc gcgatcatcc gggtcgcgag gtcgtcggtg atgccctggc cgtgctcgat
     1861 cgagtcgacc ccggcgcgca gcgcggagtc gatcccgtcc cagccgttgg cgtgcgccgc
     1921 gaccttgacc ccgagccggt gcgcctcgtc gacgatcgcc gccgcctcgg ccggcgtgaa
     1981 gttcacgatg ctgcgcaccg ggcggtccgg ccggccctcg tagaccccgt ggtcggcgta
     2041 gaacttgatc cagtcggcgc cgaagctgac ctgctcgcgc accgccttgc gcgcctggtc
     2101 cggcccgtcg atcgtctgca ccccgatcgg cagctcgagc tcccagttgt agccggcgcg
     2161 cagcgggtac atcccggtcg gcgcgaacgc gcgggtcgcg acctgcatcc gcggccccgg
     2221 caccacgccg tgcgcgatcg cgttcttgac gtcgacgtcg gcgtacatcg cgccctcggt
     2281 ctcgaggtcg cgcagcgcgg tgaacccgta catcagcgcg gtccgcgcgt tggccaccgc
     2341 ctcgatcgcg cggtacggcg cgctgtgctt gagcaggatc gcgtcgtaca ctcccgggcc
     2401 gacgtcgtcg tcgaggaaca cgtgagtgtg ggcgtcgatc atcccgggca acagcgtcgc
     2461 accgccgaga tcgacgagct tggccccggg agccttggcg gtcagctccg cggcggtgcc
     2521 gagcgcgacg atcgcgtcgc cgtcgaccag caccgcgacg ccgtcgcgcg gtcgatccga
     2581 caccccgtcg atcaggtgcg cggccttgat gatggtgggc gaggcagcgg cgggggcgct
     2641 ggcgagcagg caggcgaggg cgattctcca catggcccgc atgctctcac cgtccgcggc
     2701 gggttcgcta cttgcggcgc tggatcaggc cgcgctcgac cgcgagcttc ttgagccgct
     2761 gcttggcgcg ctcgaaccgc ttgcgcagcg cggccgcgcg ggtgtcgagg tcgccgtcgc
     2821 cgccgaggat gcgcgcgatg tccttccacg ccatgtcgcg gtcgagccgg agcagcagga
     2881 tctcgtgctc ctcgggatcg agctgctcgc gcagcaggcg gaactcgtcc ttgacctcgg
     2941 tgcgctggat ctcgcgcgtg atggtgcgga ccacggccgc catctccggg gcgatcgaca
     3001 gcggcaggtt gcgctcgcgg cggcggcgcg ggtcgcggcg gagctgggcg agcgcgttgc
     3061 gcgccagcgc gtacagccac gaccgcagcg acgactccca gcggaacttc ggcaggccct
     3121 tccagacgtc ctcgccgagc tcggcgaacg cctcggccgc gaggtcgtgg tcgccggcga
     3181 gcgcgcgcag gaagccgagc agctcgacgc cgtagccgcg cagcgcggcg gtggtcgccg
     3241 cggcgtggtc gccggcctcg cacagcgcgc ggatcgcccg ctcgctggcc tcgcgttcgg
     3301 ccggttccac cacgggatca gtctacgcta cgtggcggcg cgctcgcgcg cgtcctgctg
     3361 ctcctgcagc acgcgccaca ggatcttgcc ggtcgccgac ttgggcaggg tctcgacgaa
     3421 ctcgaagctc gccgggacct tgtaggccgc catgttgtcg cgcgcccagc gcaccaggtc
     3481 ggcctcggtg acccggccct tcgccgccgg cttgagcacg acgacggcct tgacggtctc
     3541 gccgcgccgc gggtcctggc cggcgatgat caccgcctcc tggatgtcgg ggtggccgta
     3601 cagcatggac tcgacctcgg cgggccagac cttgaagccg gcgacgttga tcatccgctt
     3661 gaggcgatcg accaggaagt agtagccgtc ctcgtcggtg cgcgccagat cgccggtgcg
     3721 gaagaaccgc ttgccgtcga gctcgacgaa gcacgccgcg ttggcctggg ggttgttcca
     3781 gtagccgtcg aacacctgcg ggccgtgcga gatgatctcg ccgacctcgc cgggcggcag
     3841 ctcgaccagg gtgtcggggt tgaccacccg cgcgtcggtg ttgaagatcg ggatgccgag
     3901 gcactgcttc ttgggccgct gcggcgggtt gatgtgggtc ggcgcgatgg tctcgctcag
     3961 gccgtagccc tcgatgaact cgagcccgca caggtcctgg agcttctggg ccaccgcctc
     4021 gggcatcgcc gcgccgccgc cgccgacctg gcgcaggctc gacaggtcgt agctggcgag
     4081 cctggggttc gacagcaggt cgacgatcat cgtcggcacc gcggtgaacc ccgtgacctt
     4141 gtaacgctgg atcagctggc ccgcgacgtc gcggtcccag cgcggcagaa ggatgatcga
     4201 gacgccggcg tagatcggcg cgttcatgcc gccctgcatg ccggtggcgt ggaagtacgg
     4261 caggatcgac aggatcgtct cgtcctggaa cgtccggttc cactgcggcc cggcgaccgt
     4321 ggtgaacatg acgctgcggt ggcggtggac gcagcccttg gggttgccgg tggtgcccga
     4381 ggtgtagggc atcacgcaca ggtcgtcggg gccggcgagg tgcgggccag gggtggcgcc
     4441 ggcggccatc gcgtcgctcc acagcgtcag cccggagcct gtctgcgact ggcgcggctc
     4501 cttgatccac gccggcacgt cgaggtcggt cggcgtcgtg aggtagtcgg agtagcacgc
     4561 gacgatcacg tgggacaggc cgttgccgac cagcggctgg atccgcgaca gcgacggcat
     4621 gctggggccg tcctgcgcgg cgaacagcac ggtcgcgccg ctgtcctgca cgatgtggcc
     4681 gacctccttg gtcaggttca tcgggttgat gggcacgacc atcgcgtcgg cccgcaggat
     4741 cgcgtagtag gcgatcacga actgcgggct gttctgcagg tacagcgcga cgcgatcgcc
     4801 gcgcttgacg ccgcacgcct gctggaggaa cccggcgagc cgctcggcct cgcggcggaa
     4861 cgccgagaag gtcaccacgc tgtcgtagaa attgaggcag gtcttgccgg ggaaccgcgt
     4921 cgccgagacc tcggcgttga accacagcga ggtctcgggc ggggtcaggt gatgcgggac
     4981 gttcggccag aactgcaggt gacgggtacc catgctgcct ccgcggttcg tgccgggccg
     5041 gccgcgaacc ggcgacaatc tatcaccggc cgggcaccgg ccgggtggcg gcgtgccccg
     5101 ccgatttggg gcactgtgtt ctgtgcgagg tggtgtgcgc ccaggggaca cccaccgtcg
     5161 ggcgccggat cagcgggccc cgccgccggg cagctgggcg agcaggtcgt cggcctgctg
     5221 gacccgctcg ggcatgtgga ggtcggcgta cgcggcgcgc gcggcgccga gcagcgggcg
     5281 ggcctcgccg gcgcgcccc
//
LOCUS       DASVOR010000696         2485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_503452,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000696 DASVOR010000000
VERSION     DASVOR010000696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2485
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503452"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VF516_13770"
     CDS             <1..194
                     /locus_tag="VF516_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108793.1"
                     /translation="HDAAHHVLDDAIARGVWRPDDAQALRATIPELSQAEASQVLADL
                     SRAINTGQVQVAMHDRPIL"
     gene            complement(220..1122)
                     /locus_tag="VF516_13775"
     CDS             complement(220..1122)
                     /locus_tag="VF516_13775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8108794.1"
                     /translation="MSETLDEQQFGAVTVLYGDRRGKYPHGNTLIVRGRDQAVAIDPS
                     LGLVARGSPPPRVDRVLHSHCHEDHFAGSFLYPETPWWFHQADLPGIRSLDALMDLYD
                     YPEPIRSTWSKVVVDQFHFTPRPDAAAFHGGDVFDLGGVTVEAIHAPGHTRGHCVFRV
                     EPVGLVYLADIDLSSFGPYYGDAWSDLEDFERTLANVRDLDARYYATFHHVGVIEGKA
                     AYLERLDRYAAVIADRERRLVAYLAEPHTLDDIVRHRFVYRPQDQVLFADGVERRSMS
                     LHLARLVRDGRVHELEPGLYRSAA"
     gene            1232..2167
                     /locus_tag="VF516_13780"
     CDS             1232..2167
                     /locus_tag="VF516_13780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8108795.1"
                     /translation="MTRLVSSFPALAVPACAAPLSPANTPGAALAAEITEHDVRVGEL
                     SLHYLRAGHGGPIVFLHGFAETSHMWRPALEALAHGHTVLAIDLPGAGGSSRPETGHD
                     KKTMAREIRALVRLLGYHRIQLVGHDIGLMVAYAYAAQFPGEVDSLTLMDAFLPGVGA
                     YQEMLLAPDSWHFHFHGPTPEALVAGRERVYLEHFWNDFAADPGRSLSEADRERYARE
                     YAKPPGIRSTMAYFAALDQDAADFAGFARTPLTMPILALGGEHASGKFLAEQAELIGS
                     HVTAVILLDAGHWLMEEAPDRTLAELVRFLHGPRS"
     gene            2258..>2485
                     /locus_tag="VF516_13785"
     CDS             2258..>2485
                     /locus_tag="VF516_13785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108796.1"
                     /translation="MAGLAGCSSPVVFHGKSTLTVTGTPAHVAQAPRVEVRDQKIEIH
                     EKIQFDNNRATIRSASFELMDEIGAVIAKHPQ"
BASE COUNT          374 a          881 c          872 g          358 t
ORIGIN      
        1 cccacgacgc cgcgcaccac gtcctcgacg atgcgatcgc acgcggcgtg tggcggcccg
       61 atgacgcgca ggcgctgcgc gcgacgatcc ccgagctcag ccaggccgag gcatcgcagg
      121 tgctcgccga cctgtcccgc gcgatcaaca ccggccaggt gcaggtcgcg atgcacgacc
      181 gtccgatcct ctgacggctg cgcgagctgt ggcctgcggt cacgccgcgc tgcggtagag
      241 gcccggctcg agctcgtgca cgcggccgtc gcgcaccagg cgcgcgaggt gcagcgacat
      301 gctgcgccgc tcgacaccgt cggcgaacag cacctggtcc tgcggccggt agacgaagcg
      361 gtgccggacg atgtcgtcca gcgtgtgcgg ctcggcgaga tacgcgacga gccgccgctc
      421 gcggtcggcg atgacggccg cgtagcggtc gagccgctcg aggtacgcgg ccttgccctc
      481 gatcacgccg acgtggtgaa aggtggcgta gtaccgcgcg tcgaggtcgc gcacgttcgc
      541 cagcgtgcgc tcgaagtcct ccagatcgga ccacgcgtcg ccgtagtacg ggccgaagct
      601 ggacaggtcg atgtcggcga gatagaccag cccgaccggc tcgacgcgga acacgcagtg
      661 accgcgggtg tgccccggcg cgtggatcgc ctcgacggtg actccgccga gatcgaacac
      721 gtcgccgccg tggaacgccg ccgcatcggg ccgcggcgtg aagtggaact ggtcgaccac
      781 caccttcgac cacgtcgagc ggatcggctc ggggtagtcg tagaggtcca tcagcgcgtc
      841 gagcgagcgg atccccggca gatcggcctg gtgaaaccac cagggcgtct cggggtacag
      901 gaagctgccg gcgaagtggt cctcgtggca gtggctgtgc agcacgcggt cgacgcgggg
      961 cggcggcgag ccgcgcgcga cgaggccgag cgatgggtcg atcgcgaccg cctggtcgcg
     1021 cccgcgcacg atcaacgtgt tgccgtgcgg atacttgccg cgccgatcgc cgtacaggac
     1081 ggtgaccgcg ccgaattgct gctcgtcgag cgtttcgctc acgcacgcat cataccttgg
     1141 ctcacggctt cgaaccgtcc ggccgcggat cgcacgatct cgcagtcgcc ggcctcgcgc
     1201 cgcatggcac ggcgactgct cgatgggttc catgacccgc ctcgtgtcct cgttccccgc
     1261 gctcgccgtc cccgcgtgcg cggctccgct gtcccccgcg aacacgcccg gcgctgcgct
     1321 cgccgccgag atcaccgagc acgacgtccg cgtcggcgag ctctcgctgc actacctgcg
     1381 ggcgggccac ggcggaccga tcgtgttcct gcacggcttc gccgagacca gccacatgtg
     1441 gcggcctgcg ctggaggcgc tggcgcacgg ccacaccgtg ctcgcgatcg atctccccgg
     1501 cgccggcggg tcgagccgtc cggagaccgg ccatgacaag aagacgatgg cgcgcgagat
     1561 ccgcgcgctc gtgcgcttgc tcggatacca ccggatccag ctggtcggtc acgacatcgg
     1621 cctgatggtc gcctacgcct acgccgcgca gttccccggc gaggtcgaca gcctgacgct
     1681 gatggacgcg ttcctgcccg gcgtcggcgc ctaccaggag atgttgctgg cgccggactc
     1741 ctggcacttc cacttccacg ggcccacccc cgaggcgctc gtcgccgggc gcgagcgggt
     1801 ctacctggag catttctgga atgacttcgc cgccgacccc gggcgatcgc tgtcggaggc
     1861 ggaccgcgag cggtatgcgc gcgagtacgc caagccgccc gggatccgct cgacgatggc
     1921 gtacttcgcc gcgctcgatc aggacgccgc cgacttcgcg gggttcgcgc ggaccccgct
     1981 caccatgccg atcctcgcgc tcggcgggga gcatgcctcg gggaagttcc tcgccgagca
     2041 ggccgagctg atcggctcgc acgtcaccgc cgtcatcctc ctcgacgccg gccactggtt
     2101 gatggaggag gcgcccgacc gcaccctcgc cgagctcgtg aggttcctcc acggacccag
     2161 gtcatgaccg cgcgaccggg atcacagcca gagtgcggcg gcgcggaata agcgctgtct
     2221 cgacggcgtc ctgcgatgga tgcgaagatg gttgatcgtg gccggactgg cgggctgtag
     2281 cagcccggtc gtgtttcacg gcaagagcac gctgacggtg acggggacgc cggcgcacgt
     2341 cgcgcaggcg ccacgcgtcg aggtgcgaga tcagaagatc gagatccacg agaagatcca
     2401 gttcgacaac aaccgggcga cgatccggtc ggcgtcgttc gagctgatgg acgagatcgg
     2461 cgcggtgatc gccaagcacc cgcag
//
LOCUS       DASVOR010000697         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_328126,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000697 DASVOR010000000
VERSION     DASVOR010000697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328126"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VF516_13790"
     CDS             complement(<1..420)
                     /locus_tag="VF516_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8108797.1"
                     /translation="MERPSIRQLESLVAVAETRSFRRAATTLGISQPALSAQVQAAEQ
                     ILGIQVFERDRRSVLITPAGEDVVSRAREALIAVDAVGDAARLRSEPLVGSLRMGVIP
                     TVAPYWLPALLPEVHRRFPRLELILREDQTSRLLALLS"
     gene            729..1295
                     /locus_tag="VF516_13795"
     CDS             729..1295
                     /locus_tag="VF516_13795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8108798.1"
                     /translation="MSIDHLKDRLPDHARDLRINLGAITSSTTLKPQQAWGTALAAAV
                     ASRNVEVVQAIAEDARLSPEAASAARGAASIMAMNNIYYRFQHMMGGGSAYADLPARL
                     RMQIIGRPGVDSVDFELWCLAASVITGCENSVRNHENMVRERGGTAEQVQDAVRIAAV
                     IHAVALTLDSTPLMILEVAVPALPIKAV"
     gene            1307..>1879
                     /locus_tag="VF516_13800"
     CDS             1307..>1879
                     /locus_tag="VF516_13800"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8108799.1"
                     /translation="MPSTSAASATEPSLFGASCGVYVRIVRLALAEKGVAYRLVERDR
                     PELDGPWPVGAQHHAFGRMPAFEHNGFVLSETGAITRYIDEGFAGPALQPASPRGRAR
                     MHQIISLLDNLAYHTLVWDIFVERVRAAFEGRAPDQTRIAAVLPIAETCLDELVELMA
                     DAPYLAGERVSLADLHAAPMMIYAHMSPEGA"
BASE COUNT          310 a          667 c          643 g          259 t
ORIGIN      
        1 ggacagcagc gcgagcagcc gcgaggtctg gtcctcgcgc aggatcagct cgagccgcgg
       61 gaaccggcgg tggacttcgg ggagcagggc gggcagccag tacggagcga ccgtcgggat
      121 gacccccatg cgcaacgagc cgaccagcgg ctcactgcgc agccgggcgg cgtcgccgac
      181 cgcgtccacc gcaatcagcg cctcacgggc ccggctcacc acgtcctcac cggcgggagt
      241 gatcaacacg ctgcggcgat cacgctcgaa gacctggatg ccgaggatct gctcggccgc
      301 ctggacctga gccgacagcg cgggttgcga gatgcccagg gtggtggcgg cccgccggaa
      361 gctgcgggtt tcagccacag caaccaggct ctcgagctgg cggatcgacg gacgctccat
      421 gataggacca cgctatcaga aggatagaat cgggtgattg gacttatcaa ctggggacgg
      481 gcactgtggc gctagagacc ccctcatgtc gatcctccga accaggtgcg cgttgctgac
      541 cgaggccggc ggcatcggat cggacaggca gcgcgtcgag cttagcgatc gcgggttcga
      601 aggcaaggtc catgggaacc agagtcgagc agtgtccgtt cacctggccg gacgttgcgg
      661 tgagggtcag cccaccctca tcgccgcgtg accggatctt cctgcagcgc acagcgtaag
      721 gagcccgcat gtcgatcgac cacctcaagg accgcctgcc cgaccacgcc agggacctac
      781 gcatcaacct cggagcgatc acgagctcga cgacgctcaa gccgcagcaa gcgtggggca
      841 ccgcgctcgc cgccgcggtc gcgtcgcgca acgtcgaggt cgtccaggcg atcgccgagg
      901 atgcgaggct gtcacccgag gcggcgtccg ccgcgcgggg cgccgcctcg atcatggcga
      961 tgaacaacat ctattaccgg ttccagcaca tgatgggcgg cggctcggcg tatgccgatc
     1021 tgcccgcccg gctgcggatg cagatcatcg gccggcccgg cgtcgacagc gtcgacttcg
     1081 agctgtggtg cctggcggcc tcggtgatca ccggctgcga gaacagcgta cgcaaccacg
     1141 agaacatggt gcgcgagcgc ggcggcaccg ccgagcaggt ccaggacgcg gtccggatcg
     1201 ccgcggtgat ccacgcggtc gcgctgaccc tcgacagcac cccactgatg atcctggagg
     1261 tagccgtccc cgccttgccc atcaaggccg tgtgagggtg acccggatgc ccagcacctc
     1321 cgcagcgagc gcgaccgagc ccagcctgtt cggcgccagc tgcggcgtct acgtccggat
     1381 cgtccggctc gcgctcgccg agaagggcgt cgcgtaccgg ctggtcgagc gcgaccgccc
     1441 cgagctcgat ggcccgtggc cggtcggggc gcagcaccac gcgttcggcc ggatgccggc
     1501 gttcgagcac aacgggttcg tgctctccga gaccggcgcg atcacgcgct acatcgacga
     1561 gggcttcgcc gggccggcgc tgcagcccgc gagcccgcgc ggccgcgccc gcatgcatca
     1621 gatcatctcg ctgctcgaca acctcgccta ccatacgctg gtctgggaca tcttcgtcga
     1681 gcgagtgcgc gccgcgttcg agggccgcgc gcccgaccag acgcggatcg ccgccgtact
     1741 gccgatcgcc gagacctgcc tcgacgagct ggtcgagctc atggccgacg ccccgtacct
     1801 cgccggcgag cgggtcagcc tggccgatct ccacgccgcg ccgatgatga tctacgccca
     1861 catgtccccc gagggcgcc
//
LOCUS       DASVOR010000698         5411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_6974,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000698 DASVOR010000000
VERSION     DASVOR010000698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6974"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF516_13805"
     CDS             <1..327
                     /locus_tag="VF516_13805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108800.1"
                     /translation="GGRLAGACEDDRDEQEPGFHGRRILRHGGPDRQGFPITPARLAR
                     TIHHAAGCDRRGAGISGCTAGSRSSSTSSTILTALPGVRPVPLCPLEILRDHAIAFTM
                     KITTCR"
     gene            complement(367..1290)
                     /locus_tag="VF516_13810"
     CDS             complement(367..1290)
                     /locus_tag="VF516_13810"
                     /inference="COORDINATES: protein motif:HMM:NF025619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HEX8108801.1"
                     /translation="MVAAVTRTRFDQAGKQLVRDALEGHCSVETDAEVPADTRRIDLW
                     VTPREASAPLPDHLGLLGRITSGSVTLEFFHNTPSGEELRVCLIKHGEFRHFLSRRKT
                     LPPLPIQWVISSGRPDAGIDGLGFRPITGGPRGIYESPPLHYTRLVVVNELHVARDTL
                     LVRLLGAGSVLKQAIDELQALPAEAPERRLALPVLLRLRLTVPIDPAQQTSDDQEFLM
                     NTQDIVETWRREAIQEGVEQGVKQGLEQGVKQGLEQGERKLLLRQLRRRFGAEVDGEI
                     ERRVAAASAEQVEVWAERVLSAATLTELLAD"
     gene            complement(1925..3127)
                     /locus_tag="VF516_13815"
     CDS             complement(1925..3127)
                     /locus_tag="VF516_13815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108802.1"
                     /translation="MAPGMIVEGDVVAVHGYWTDDRSRIVTEATVQTPDGPVVVSQLG
                     GSADGIGMITIPGPPRLELGMQVAVAAHTDLDRSQREHVVLDSVKVLADPPDYVRRGP
                     TRAGHFLYWESRCVFLTIDDAGTIAIPGDGEFPVIDASIATWNDAATPSCSYLKLMSD
                     GRQPLEVGNDHVNLIKFRDTTWGRPAVGNDPPRMYPAQAAAFTTVLYVDDGSARDGAI
                     YDADIELNGVYFAIAVNGQTSANSQLCHAELQNTLTHELGHLIGLEHTCLALGDPQRI
                     DGQGNPVPDCSAVAATPTLPASMQILDATMYPFQDCGETKKATLSSDDIQALCGIYPV
                     ARDPGICSRVGADGLVPPDDVGADSAVPSGGHGCCDASASARPDASLVLAGATFVLVM
                     RRRRAART"
     gene            3215..4540
                     /locus_tag="VF516_13820"
     CDS             3215..4540
                     /locus_tag="VF516_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HEX8108803.1"
                     /translation="MFDPAIYAARREAYMQAIGPQAVAVVCSLPERVRNGDAHYVFRQ
                     HSDLVYLTGFLEPEAVLVLRPGAEKEKVVMFVRPSDPEAELWDGRRAGLDGARQRYGA
                     DAAYPAAELPARLWELIANTEELHYSLGLDEDMDRLIGAALVRLRRLEKRGKRPPRAV
                     VDPRVGLHELRLHKRPEELAALATAAQITCDAHRLAMRHGRPGSQEHELEALINHTFR
                     SRGGHGPGYSTIVGAGANATILHYIANASPIRDGDLVLIDAGCEYQHYTADVTRTWPA
                     SGRFTAPQRRVYDAVLATQKSAIAMVRPGITLDDIHNHCVKQLTAAMLELGLLTGTVD
                     ERVTDQGYRKFYMHGTSHWLGLDVHDVGAYTREGKPRPLEPGMVITVEPGLYIAAGSD
                     APAELRGIGVRIEDDVVVTEAGHEVLTHACPKEVEELEAACLAAPASAA"
     gene            4760..5245
                     /locus_tag="VF516_13825"
     CDS             4760..5245
                     /locus_tag="VF516_13825"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8108804.1"
                     /translation="MKHRPALERSARRLCRNRHDAEDLVQATLVRAWQARAQFLPGGN
                     IGGWLYRILSNHHRDNLRRKSGIQVPLPDEVPDLASEDDDSSPLLAMPPEVVLEALHQ
                     LPDELRQPLELSVIHGKRYREIAEQLNIPINTVGTRIRRARHRLLDQFADDQRRAGGG
                     R"
BASE COUNT          851 a         1892 c         1868 g          800 t
ORIGIN      
        1 ggcgggcggc ttgctggagc atgcgaggac gaccgcgacg agcaggaacc tggctttcat
       61 ggccggcgca tcctacgcca cggaggtccg gaccggcagg gcttcccgat cacgccggcc
      121 cggctggccc ggacaattca ccacgccgcc ggctgcgacc gtcgcggcgc gggcatctcg
      181 ggttgtacgg ccggttctcg ttcgtcaagt acgtccagta ctatcctcac tgcgcttccg
      241 ggcgtgcgcc ccgtaccgct gtgtcccttg gagatccttc gcgatcatgc gatcgccttc
      301 acgatgaaga tcacgacgtg ccggtagagc aactcaaccg cgcgggcaag ccggtgcgat
      361 cggtgttcag tctgccagga gctcggtgag cgtcgcggcc gacaacactc gctcggccca
      421 gacctcgacc tgctcggcag atgccgccgc aacgcggcgc tcaatctcgc cgtcgacctc
      481 ggctccaaag cgtcgccgta gctggcgcag gagcagcttg cgttcgccct gttccagacc
      541 ttgcttgacg ccttgttcca gaccttgctt gacgccctgc tcgacgccct cctggatcgc
      601 ttcccgccgc caggtctcga cgatatcctg ggtgttcatc agaaactcct ggtcatcgct
      661 ggtctgctgc gcagggtcga tcggcacggt caagcgtaag cgtagcagaa ccggcagcgc
      721 gagcctacgc tccggtgcct ccgccggcag ggcctgaagc tcgtcgatcg cctgctttag
      781 taccgagccg gcaccgagca gacgcacgag cagcgtgtca cgcgccacgt gcagttcgtt
      841 caccaccacg agccgcgtgt agtggagcgg cgggctttca tagatgccgc gtggcccgcc
      901 agttatcgga cggaatccca ggccatcgat gccggcgtca gggcggcctg aagagatcac
      961 ccactggatc ggaagcggcg gaagcgtctt gcgtcgcgac aggaaatgcc ggaactcgcc
     1021 gtgcttgatc aagcacacgc ggagctcttc accactgggc gtgttgtgga agaactcgag
     1081 cgtgaccgag ccactggtga tgcgccccag gagacctaga tggtcgggca gcggcgcgct
     1141 ggcctcgcgg ggggtgaccc agagatcgat gcgacgcgtg tcggccggga cttccgcgtc
     1201 ggtctcgacg gagcagtggc cttcgagcgc atcgcgaacc agctgcttgc cggcctgatc
     1261 gaatcgcgtg cgcgtcaccg cggcgaccat cgcacgccgc gatgcgaggg caaagaaacg
     1321 gacgtgaacg cagccgcctg aaagcgtttg catgcgttgg aacgccgacg cttcgtacgc
     1381 tttgatgctg gccaggcgag cagcagcgga cgatgcaagc gggagcgagg caggcgaact
     1441 gcagcggcga ggcgagccgc agcggccagg gcgcgcggcg gcgcgccgat ccaaatcgcg
     1501 cgcagccact ccggcatcgc ggcagcccct gtcatttacc ggccattcca atcgactgcc
     1561 atcgtggttc gccggcgaac ctccatagga gctgagcctt ccaggctggt cgattccagc
     1621 accggacgtg tcgtagtcga ccccgtcgtg tcgcctgatg gatagcatcg aggcgatcga
     1681 tgaagcagcc attgcaggcc aggtcggcga cgaagaagca cgtgatcctg tttctggcgg
     1741 gtctgggacg cggccaccgg gaagccgctc agccgcaagc tagaacatca gttggcggta
     1801 tcgaaggcca gagggttctc gatcgacctc tcgggcgtgc gccccgatct gcctgacgcc
     1861 gcgagccacc gcgagcaaag cgagcggaag ggagcgaccg agacgtcgtg atgaatggcc
     1921 cgggctaggt gcgcgccgct ctgcgccgcc gcatcaccag gacgaacgtc gctccggcga
     1981 ggaccaggct cgcatccggc cgggcgctgg cggacgcgtc gcagcagccg tggccgcccg
     2041 acggcaccgc gctatcggcg ccgacgtcgt cgggtgggac caggccgtcg gcgcccacgc
     2101 ggctgcagat ccccgggtcc cgcgcgaccg ggtagatgcc acacagcgcc tggatgtcgt
     2161 cgctggacag cgtcgccttc ttggtctcgc cgcagtcctg gaacgggtac atcgtggcgt
     2221 cgaggatctg catgctggcg ggcagcgtcg gtgtggccgc caccgccgag cagtccggca
     2281 ccgggttgcc ctggccatcg atccgctgcg gatcgccgag cgccaggcag gtgtgctcga
     2341 ggccgatcag gtggccgagc tcgtgggtga gggtgttctg cagctcggcg tggcacagct
     2401 ggctgttggc gctggtctgt ccgttcaccg cgatcgcgaa gtagaccccg ttgagctcga
     2461 tgtcggcgtc gtagatcgca ccgtcgcgcg cgctgccgtc gtcgacgtac agcaccgtgg
     2521 tgaaggccgc ggcttgcgca gggtacatgc gcggcggatc gttgccgacc gccggccgcc
     2581 cccacgtggt gtcgcggaac ttgatcaggt tgacgtggtc gttgccgacc tcgagcggct
     2641 ggcgcccgtc gctcatgagc ttgaggtacg agcacgacgg cgtggccgcg tcgttccagg
     2701 tcgcgatcga cgcgtcgatc accgggaact ccccgtcgcc ggggatcgcg atggtccccg
     2761 cgtcgtcgat ggtcaggaac acgcagcgcg attcccagta caggaagtgg ccggccctgg
     2821 tcggcccgcg gcggacgtag tcgggtggat cggcgagcac cttgacgctg tcgagcacca
     2881 cgtgctcgcg ctgcgatcgg tcgaggtccg tgtgcgcggc gaccgcgacc tgcatcccga
     2941 gctcgagcct cggtggcccc gggatcgtga tcatgccgat gccgtcggcg ctgccgccga
     3001 gctggctcac cacgaccggc ccatcgggcg tctgcaccgt cgcctcggtc acgatccgcg
     3061 agcggtcgtc ggtccagtag ccatgcacgg cgaccacgtc gccctccacg atcatccccg
     3121 gtgccatgcc ggcaatgtac cagagccgcg cgatggcaag ctccgggagt ggaagcatcg
     3181 ggaccgagcg gacccgcgtt gctatagtca ccgcatgttc gacccggcga tctacgccgc
     3241 ccggcgcgag gcgtacatgc aagcgatcgg cccccaggcg gtggccgtgg tctgctcgct
     3301 gcccgagcgc gtgcgcaacg gcgatgcgca ctacgtgttc cgccagcact ccgacctggt
     3361 ctacctgacc ggcttcctcg agccggaggc ggtgctggtg ctgcggcccg gcgccgagaa
     3421 ggagaaggtc gtgatgttcg tccggccgag cgacccggag gccgagctgt gggacggccg
     3481 gcgcgccggg ctcgacggcg ccaggcagcg gtacggcgcc gacgccgcgt accccgccgc
     3541 cgagctgccc gcccggctgt gggagctgat cgccaacacc gaggagctgc actacagcct
     3601 cgggctcgac gaggacatgg accgcctgat cggcgccgcg ctggtccggc tgcgccggct
     3661 ggagaagcgc ggcaagcgcc cgccgcgcgc ggtggtcgat ccgcgggtcg gcctgcacga
     3721 gctccggctc cacaagcggc ccgaggagct cgccgcgctc gcgaccgcgg cgcagatcac
     3781 gtgcgacgcg caccggctcg cgatgcgcca cggccggccc ggcagccaag agcacgagct
     3841 cgaggcgctg atcaatcaca cgttccgcag ccgcggcggc cacggcccgg gctacagcac
     3901 catcgtcggc gcgggcgcca acgcgacgat cctgcattac atcgccaacg cgagcccgat
     3961 ccgcgacggc gacctggtcc tgatcgacgc cggctgcgag taccagcact acaccgccga
     4021 cgtcacccgg acgtggccgg cgagcggccg gttcaccgcg ccgcagcgcc gggtctacga
     4081 cgccgtgctc gcgacccaga agtcggcgat cgcgatggtc cggcccggga tcacgctcga
     4141 cgacatccac aaccactgcg tcaagcagct caccgcggcg atgctggagc tcggcctgct
     4201 caccggcacg gtcgacgagc gggtcaccga ccagggctat cgcaagttct acatgcacgg
     4261 cacctcgcac tggctcggcc tcgacgtcca cgacgtcggc gcgtacaccc gcgagggcaa
     4321 gccgcgtccg ctcgagcccg gcatggtgat cacggtcgag cccgggctct acatcgccgc
     4381 cggcagcgac gccccggccg agctccgcgg catcggcgtg cggatcgagg acgacgtcgt
     4441 ggtcacggag gccggccacg aggtgctgac ccacgcctgc cccaaggagg tcgaggagct
     4501 ggaggcagct tgcctcgccg caccggcgtc cgcggcatga cgtaggtggt tcaccgagaa
     4561 aagtcgccga aaagaaagtc ggggtcggcg cgtctatcga agtggtcgct ccatgtcccc
     4621 cgcggcgcct atcgccttga cccttcgcgg ctcgcgtgcc aacgtcgggc tgccgtttct
     4681 tgaacacggt ggccaatgga ggccaatgcg cgggggtcgc gggaccgaca gtgatcaacc
     4741 cgacttcccc gacgagctcg tgaagcaccg accggcgctg gagcgatccg cgcgccggct
     4801 gtgtcggaac cggcacgacg cggaagatct cgtccaggcg accctggtac gggcgtggca
     4861 agcgcgcgcg cagttcctgc ccggcggcaa catcggcggc tggctgtacc ggatcctgtc
     4921 caaccaccac cgcgacaatc tgcgccgcaa gagcggcatt caagttccgc tgcccgacga
     4981 ggtcccggat ctcgcgtccg aggacgacga cagctcgccg ctgctggcga tgccgcccga
     5041 ggtcgtgctg gaggcgctgc accagctgcc cgacgagctg cgccagccgc tcgagctgtc
     5101 cgtgatccac ggcaagcgct accgcgagat cgccgagcag ctgaacatcc cgatcaacac
     5161 cgtcggcacg cggatccggc gcgcgcgaca ccggctgctc gatcagttcg cggacgacca
     5221 gcgccgcgcg ggaggtggcc gatgaccgag cgctgtgacc agctgcatgc ctacgtcgac
     5281 ggcgagctcg ccgaggccga ggccgccgag ttcgaggccc acctcgcgac ctgcgaggac
     5341 tgcctcgccg agctgccccg gctgctcgcg ctgctcgagg cgctcgacgg cgccgcccgg
     5401 gtcgcccgga c
//
LOCUS       DASVOR010000699         4484 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415238,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000699 DASVOR010000000
VERSION     DASVOR010000699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415238"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..992)
                     /locus_tag="VF516_13830"
     CDS             complement(51..992)
                     /locus_tag="VF516_13830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108805.1"
                     /translation="MADPSVGLADPPATFSCGTCGAGLSFAGVRSAACPYCASPHVVE
                     RPPDAGRPQPELVVAFTGDAAFARRQLERWLGSRTWFADARIQRARVEDLRGVYVPGY
                     LYSAVAHTDYTASIGEQYIEIETRETTDAEGHKKTEIRTVTRTEYRPLSGHHVGYVTD
                     VLVSASAGLTDAELAAIEPYDYLHLRRYAPALIAGWIAEEFARDPDHCRRASREAAID
                     DVGLRLRRFMPGDGHSDLSWSTSVTWESLAPVLVPVWVLAVRYRDDRRPLRVVINGQT
                     GAIAGKVPLAWWKVALAVLVIAGIAAAIAWLWHGGAP"
     gene            complement(1059..1958)
                     /locus_tag="VF516_13835"
     CDS             complement(1059..1958)
                     /locus_tag="VF516_13835"
                     /inference="COORDINATES: protein motif:HMM:NF016946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HEX8108806.1"
                     /translation="MQRFLIFLERHVLVPQPIARLVVVRIAASLAIVGFMSSRIVHAD
                     DWLSVAGFRLPEIADYRQPLALPGLPVWAAWAFGALLAISGLATAAGAFTRWSSGVFA
                     ALLTYVALADRLEAFTVSKLAPMVALVLCLTPSGARASVDAWRLRRRDPAAVLPDQVS
                     GGCVLFFQILLPVFYFSSGRCKAHGDWKSDHFVLWTHLHDSYQTAVSWFIANHLPVEM
                     WRVLQSAVLVFEYAAPVWFGLPWTRPYALVFGVGMHLMIGLMFGPVIWFSLLMMTLLI
                     ASYAPATWLARALHLRPSRPMSP"
     gene            complement(1943..2506)
                     /locus_tag="VF516_13840"
     CDS             complement(1943..2506)
                     /locus_tag="VF516_13840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108807.1"
                     /translation="MRKPEPPPIARGPGPRWFRFGLIALALVYYGALVHHPPDVKWVR
                     PAAFFTDATCLFPRSDVIAQEYRLEVWVCGREWALIDPAPYFPIRPDDKESRFQRLAY
                     FYSRTRPAMQALDAYVSAGHAAGADDGVTGTIGGIRLVKVERPLPAPGDPVERYHFDP
                     RAPIPPEQRHDLYYTPESQRKRRCSGS"
     gene            2868..3908
                     /locus_tag="VF516_13845"
     CDS             2868..3908
                     /locus_tag="VF516_13845"
                     /inference="COORDINATES: protein motif:HMM:TIGR04552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04552 family protein"
                     /protein_id="HEX8108808.1"
                     /translation="MVSPVLPRVARIASTSELTLADIDAVRLLLRGDSVIDWHRLNYA
                     DHAEVDRFLRLNEFDPDNDEEMARLEDVRSDAVEYLSRAFAMAIPDEVAADLPARDLF
                     LVASSHGPHQKWACVVLKVMHIIHHINGRGALLTLSVSDEIIFREIELKVMQVVEQLR
                     AAGAPLAEWEWSRKPKDSQITKLLAKRSTIAANIYDKLRFRLIVPTPDDLVPMIATLT
                     RQLIPFNYVVPGESVNQLVDLQHVLQDRLDGAQAPTENPYNEFSGPEYRIVNFVADLP
                     LRVDRLLSRAESPPDNTHVVFVLTEFQIADKATSLRNESGASSHEAYKARQNERVRMR
                     LFREKDDPLPPD"
     gene            complement(3927..>4484)
                     /locus_tag="VF516_13850"
     CDS             complement(3927..>4484)
                     /locus_tag="VF516_13850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108809.1"
                     /translation="AVVPFLRVESWPLVIGFFAAMAGMAGISELSYRRGASVAPVALA
                     ATFATALLVSRVAGPFVLTPVVIEGIALGLIATGPLIGRPWIVIAWLALALVVPIALE
                     AAGVFAQTWWFDGDTLQIHSAVVHGSDAAREVALIAANLLFIVMAVLFARATHRDRRD
                     AERRLHIQAWHLRHLLPDRTRTAPA"
BASE COUNT          729 a         1591 c         1603 g          561 t
ORIGIN      
        1 caggtcgccg tcctccagcg cggtctggca ccgcgtgcac gccgcggcgg tcatggcgcg
       61 cctccgtgcc acagccaggc gatcgccgcg gcgatgccgg cgatgaccag gaccgccagc
      121 gcgaccttcc accacgcgag cggcaccttg ccggcgatcg ccccggtctg gccgttgatc
      181 acgacgcgca gcgggcggcg gtcgtcgcgg tagcgcacgg cgagcaccca gaccggcacg
      241 aggaccggcg cgagcgactc ccaggtcacg gacgtgctcc acgacaggtc actgtggcca
      301 tcacccggca tgaaccggcg cagccgcagg ccgacgtcgt cgatcgcggc ctcgcggctc
      361 gcccgccggc agtggtcggg gtcgcgcgcg aactcctcgg cgatccagcc ggcgatcagc
      421 gccggcgcgt agcggcgcag gtggaggtag tcgtagggct cgatcgcggc gagctcggcg
      481 tcggtcagcc cggccgacgc gctgaccagg acgtcggtga cgtagccgac gtgatggccg
      541 gacagcgggc ggtactcggt gcgggtcacc gtgcggatct cggtcttctt gtggccctcg
      601 gcgtcggtgg tctcgcgggt ctcgatctcg atgtactgct cgccgatcga cgccgtgtag
      661 tcggtgtgtg cgaccgccga gtacaggtac cccggcacgt agacgccgcg caggtcctcg
      721 acccgggcgc gctggatccg cgcgtcggcg aaccaggtcc ggctgccgag ccagcgctcg
      781 agctggcgcc gggcgaacgc cgcgtcgccg gtgaacgcga cgaccagctc gggctgcggc
      841 cgcccggcat ccggcgggcg ctcgacgacg tgggggctcg cgcagtacgg gcaggccgcg
      901 ctgcgtacgc cggcgaacga caggcccgcc ccgcaggttc cgcacgagaa ggtcgcaggg
      961 ggatcggcca gaccgacgct cggatccgcc atcctctcag cgtagccgag accgcgcgtg
     1021 ggatcatcct gcccggcacg gccgccgcgc gcccggggtc acggggacat cgggcgcgac
     1081 gggcgcagat gcagcgcgcg ggccagccag gtcgccggcg cgtagcttgc gatcaggagc
     1141 gtcatcatga gcagcgagaa ccagatcacc gggccgaaca tgaggccgat catgaggtgc
     1201 atcccgacgc cgaacaccag ggcgtagggc cgggtccacg gcaggccgaa ccacaccggc
     1261 gcggcgtact cgaacaccag cacggcgctc tggagcacgc gccacatctc gacggggaga
     1321 tggttggcga tgaaccagct caccgcggtc tggtacgagt cgtggaggtg ggtccacagc
     1381 acgaagtggt cggacttcca gtcgccatgg gccttgcacc ggcccgacga gaagtagaac
     1441 accggcaaga ggatctggaa gaacaggacg caaccgccgg acacctggtc gggcagcacc
     1501 gcggccggat cgcgccgccg cagccgccag gcgtcgaccg aggcccgcgc gccgctcggc
     1561 gtcaggcaca gcacgagcgc gaccatcggc gcgagcttgg acacggtgaa cgcctcgagc
     1621 cggtcggcga gcgcgacgta ggtgagcagc gcggcgaaca caccgctcga ccaccgcgtg
     1681 aacgcgcccg cggcggtcgc cagcccggag atcgcgagca gcgccccgaa cgcccacgcc
     1741 gcccacaccg gcaggcccgg cagggcgagc ggctggcggt agtccgcgat ctcgggcagc
     1801 cggaacccgg cgaccgacag ccagtcgtcg gcgtggacga tccgggacga catgaagccg
     1861 acgatcgcga gcgacgcggc gatccggacc acgacgagcc gcgcgatcgg ctgcggcacg
     1921 agcacgtggc gctcgaggaa gatcaggaac cgctgcatcg ccgcttcctc tgcgactcgg
     1981 gggtgtagta gaggtcgtgg cgctgctcgg gcgggatcgg cgcgcgcgga tcgaagtggt
     2041 agcgctcgac cggatcgccc ggggcgggca gcgggcgctc gaccttgacc agccggatcc
     2101 cgccgatcgt gccggtgacg ccatcatcgg cgccggcggc gtgcccggcc gagacatagg
     2161 cgtcgagcgc ctgcatcgcc ggccgggtgc ggctgtagaa gtacgccagc cgctggaacc
     2221 gcgactcctt gtcgtcgggc cggatcggga agtagggcgc ggggtcgatc agcgcccact
     2281 ctcggccgca gacccagacc tccagccggt actcctgagc gatcacgtcc gacctgggaa
     2341 acaggcaggt cgcatcggtg aagaacgccg cggggcggac ccacttgacg tccggcgggt
     2401 ggtgaaccag cgcaccgtag tagaccagcg cgagcgcgat gagcccgaac cggaaccagc
     2461 gtgggccggg gcctcgggcg atcggcggag gttcaggctt cctcacagaa ggcacgctac
     2521 ggcggatgtg gccggatcgg tgtgaaatgt aacaggaccc ggcaggaagc ggacgggatg
     2581 gctactcgat cagccacctg cgcggcctgg ccattccctg ccgagatctg cccccggcgt
     2641 cgccgggagt gggcgcggcg tggcgccaca tgccgtttgt tggactcgcg ttcccgccgc
     2701 ggcgagaatt tgccgcggaa cacgcggacg acccaccgcg ttgttgctag caagggacgt
     2761 acatgatcgc cgtcattgga aacgtgatcg ccgggctcgg cgtgctcagc ctgttcaccg
     2821 ccacgttgcg gctgtccaag ccccgcaagc gatcatagac tcgcagggtg gttagccccg
     2881 tcctgccacg ggtggcgcgg atcgcctcga cctcggagct gacgctcgcc gacatcgacg
     2941 ccgtgcgcct gctcctgcgc ggcgactcgg tgatcgactg gcaccggctg aactacgccg
     3001 accacgccga ggtcgaccgg ttcctccggc tcaacgagtt cgatcccgac aacgacgagg
     3061 agatggcgcg gctggaggac gtgcgcagcg acgcggtcga gtacctgtcg cgggcgttcg
     3121 cgatggcgat ccccgacgag gtcgccgccg acctgccggc gcgcgacctg ttcctggtcg
     3181 cgtcgagcca cggcccgcac cagaagtggg cgtgcgtcgt gctcaaggtg atgcacatca
     3241 tccaccacat caacggccgc ggcgcgctgc tcacgctgtc ggtgtccgac gagatcatct
     3301 tccgtgagat cgagctcaag gtcatgcagg tggtcgagca gctgcgcgcc gccggggcgc
     3361 cgctcgccga gtgggagtgg agccgcaagc ccaaggactc ccagatcacc aagctgctcg
     3421 ccaagcgctc gacgatcgcc gccaacatct acgacaagct gcggttccgg ctgatcgtgc
     3481 cgacccccga cgacctcgtg ccgatgatcg ccacgctgac ccgccagctg atcccgttca
     3541 actacgtggt gcccggcgag tcggtcaacc agctggtcga cctccagcac gtgctgcagg
     3601 accggctcga cggcgcgcag gcgccgaccg agaacccgta caacgagttc tccggccccg
     3661 agtaccggat cgtcaacttc gtcgccgacc tgccgctgcg ggtcgatcgc ctgctgtcgc
     3721 gcgccgagtc gccgccggac aacacccacg tcgtgttcgt gctcaccgag ttccagatcg
     3781 ccgacaaggc gacctcgctg cgcaacgagt ccggcgcgtc gagccacgag gcctacaagg
     3841 cgcgccagaa cgagcgggtg cggatgcggc tgttccgcga gaaggacgac ccgctgccgc
     3901 cggactgagg tgcggcgctg ggcgggtcac gccggcgcgg tgcgcgtgcg atccggcagc
     3961 aggtgccgga ggtgccaggc ctggatgtgg agccggcgct cggcgtcgcg gcgatcgcgg
     4021 tgggtcgcgc gcgcgaacag caccgccatc acgatgaaca gcaggttcgc cgcgatcagc
     4081 gcgacctcgc gcgccgcgtc gctgccgtgg accaccgcgg agtggatctg cagcgtgtcg
     4141 ccgtcgaacc accaggtctg cgcgaacacg cccgccgcct cgagcgcgat cggcacgacc
     4201 agcgcgagcg ccagccatgc gatcacgatc cacggccggc cgatcagcgg cccggtcgcg
     4261 atcagcccga gcgcgatgcc ctcgatcacg accggggtca gcacgaacgg cccggcgacc
     4321 cgcgacacca gcagcgcggt cgcgaacgtc gcggcgagcg cgaccggcgc gaccgatgcg
     4381 ccgcgccggt acgacagctc cgagatcccc gccatcccgg ccatcgccgc gaagaacccg
     4441 atcacgagcg gccagctctc gacccggagg aacggcacca cggc
//
LOCUS       DASVOR010000700         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_65080,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000700 DASVOR010000000
VERSION     DASVOR010000700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65080"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VF516_13855"
     CDS             <1..276
                     /locus_tag="VF516_13855"
                     /inference="COORDINATES: protein motif:HMM:NF020135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8108810.1"
                     /translation="ATRRSIDEMVAHNRRSAKSHSGIWRDLAVRKRKTEVDAQIAVVA
                     EIAALHGRTTPVLERLVRLVHEIEDGQRPQGRANLDSLLGALAEAAP"
     gene            273..1043
                     /locus_tag="VF516_13860"
     CDS             273..1043
                     /locus_tag="VF516_13860"
                     /inference="COORDINATES: protein motif:HMM:NF009466.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8108811.1"
                     /translation="MNIEFTGKTVIVTGGAHGLGRAIAHAFAARGARTWTCDILADGL
                     AQTAAQASPAAGGCLETAVVDVTDRAAVGQFVDRIAEATGRIDVLVNNAGGVCGQVGQ
                     PIEAVTQAQWQVIFDVNVHGSFHCAQAVAPHMKAARAGRIVHISSGAGLGVSLTGIQA
                     YASAKAAQIGLTRQLAHELGPFGITVNSIAPGFVRSNPTTERQWEAYGAEGQQRLIER
                     IALRRLGSPADIAHAVLFFASDHAAWITGQVLSVNGGA"
     gene            complement(1044..>1576)
                     /locus_tag="VF516_13865"
     CDS             complement(1044..>1576)
                     /locus_tag="VF516_13865"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8108812.1"
                     /translation="DWLALYYAARTLLAANFAAEIKGIVARVGREPGDDELGPLARRS
                     YEAGKQVSGADALAATQRMRALAWQLLEQFSDFDVFLTPVLGTLPPRTDELDPLAGDL
                     RAFDQRTAATFPFTPPFNMTGQPSISLPLVQSPSGLPIAMMFTARYGDEATLFRLAAQ
                     LEQARPWADRHPPIWG"
BASE COUNT          223 a          542 c          616 g          195 t
ORIGIN      
        1 gcgacccggc gctcgatcga cgagatggtc gcgcacaacc ggcggtcggc gaagtcgcac
       61 agcgggatct ggcgcgacct cgcggtgcgc aagcgcaaga ccgaggtcga cgcgcagatc
      121 gcggtcgtcg ccgagatcgc ggcgctgcac ggccggacca cgccggtgct cgagcgcctg
      181 gtccggctcg tccacgagat cgaggacggc cagcggccgc agggccgtgc caacctcgat
      241 tccctcctcg gtgccctcgc cgaggcggcg ccgtgaacat cgagttcacc ggcaagaccg
      301 tgatcgtgac cggcggcgcg cacggcctcg gccgcgcgat cgcacacgcg ttcgcggcgc
      361 gcggcgcgcg gacatggacg tgcgacatcc tggcggacgg cctggcgcag accgcggcgc
      421 aggcgagccc ggcggcgggc ggctgcctcg agaccgcggt ggtcgatgtc accgaccggg
      481 cggcggtcgg gcagttcgtc gatcgcatcg ccgaggcgac cgggcggatc gatgtcctgg
      541 tcaacaacgc cggcggggtc tgcgggcagg tcggccagcc gatcgaggcg gtgacccagg
      601 cgcagtggca ggtgatcttc gacgtcaacg tccacggcag ctttcactgc gcgcaggcgg
      661 tggcgccgca catgaaggcg gcgcgcgccg gccggatcgt ccacatctcg agcggcgcgg
      721 ggctcggcgt cagcctgacc gggatccagg cctacgcgtc ggccaaggcg gcgcagatcg
      781 ggctgacccg ccagctcgcc cacgagctcg ggccgttcgg gatcacggtc aacagcatcg
      841 cgcccgggtt cgtgcgctcg aacccgacca ccgagcggca gtgggaggcg tacggcgccg
      901 agggccagca gcgcctgatc gagcgcatcg cgctgcggcg gctcggatcg ccggccgaca
      961 tcgcccacgc ggtgctgttc ttcgcctcgg accacgcggc gtggatcacc gggcaggtcc
     1021 tgtcggtgaa cggcggcgcg tagtcagccc cagatcggcg ggtggcggtc ggcccacggc
     1081 cgcgcctgct cgagctgcgc ggcgagccgg aacagcgtgg cctcgtcgcc gtagcgcgcg
     1141 gtgaacatca tcgcgatcgg caggccgctg ggggactgca cgagcggcag cgagatcgac
     1201 ggctggccgg tcatgttgaa cggcggggtg aacgggaagg tcgcggcggt gcgctgatcg
     1261 aaggccctga gatcgcccgc gagcggatcg agctcgtcgg tgcgcggcgg gagggtgccg
     1321 agcaccgggg tgaggaagac gtcgaagtcg gagaactgct cgagcagctg ccaggccagc
     1381 gcgcgcatgc gctgcgtggc ggccagcgcg tcggcgccgc tgacctgctt gccggcctcg
     1441 tagctgcgcc gcgccagcgg gccgagctcg tcgtcgcccg gctcgcgccc gacgcgggcg
     1501 acgatgccct tgatctcggc cgcgaagttg gccgccagca gggtgcgggc ggcgtagtac
     1561 agcgcgagcc agtcga
//
LOCUS       DASVOR010000701         1554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_562289,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000701 DASVOR010000000
VERSION     DASVOR010000701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1554
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562289"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF516_13870"
     CDS             <1..214
                     /locus_tag="VF516_13870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108813.1"
                     /translation="VRLARAEALHATGAHDVARHAIADARARLLAIADKICDPDYRQS
                     FLHGVPENARTLALAQTWVGTAAPDR"
     gene            634..1257
                     /locus_tag="VF516_13875"
     CDS             634..1257
                     /locus_tag="VF516_13875"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108814.1"
                     /translation="MELADLLEERFEIEDRIQGGGMGEVFRARDRVSGEPVAIKVIAS
                     GHDPRGARFAREVRLLSELSHPAIVRYVAHGVTAPGVPFLVMEWLDGEDLRRRLEREP
                     LTIGESVALAARVAEALGVAHARGIVHRDLKPSNLFLPDRRIDQVKVLDFGIARRDGL
                     THLTRTGTLIGTPGYMAPEQARSHEVVDARADVFALGCVLFECLTGS"
     gene            1342..>1554
                     /locus_tag="VF516_13880"
     CDS             1342..>1554
                     /locus_tag="VF516_13880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108815.1"
                     /translation="MPEDLDALVARMLARDPALRPSDGASLAAALAALDPRAHSSIVP
                     PRGQSVRRPALTDGERRLLSVVLLGPA"
BASE COUNT          219 a          541 c          565 g          229 t
ORIGIN      
        1 cgtccggctc gcccgcgccg aggcgctgca cgcgaccggc gcacacgacg tcgcgcggca
       61 cgcgatcgcc gacgcccgcg cgcgcttgct ggccatcgct gacaagatct gcgacccgga
      121 ctatcgccag agcttcctcc acggcgtccc cgagaacgcc cggacgctcg cgctggcgca
      181 gacctgggtc ggcaccgcgg cgccggatcg ctaggcgttg ccaggcgcgc caggacggaa
      241 agcgcgcaca tcgcgtctgt ccggtgggct ggctggcacg atgagcagac cgcatacctg
      301 atccgtcggc gaacattgtt attgctgctc actaccaaga tcacatccat tcgttgaagc
      361 gttgttcgag acatgattgc cggtagctat cgccgaccgc cgatcggcga cgccgcgctg
      421 cgcctgtgag gcatcgggcc ggcgcgacgc gcctggagca ttgcgaagca ggtcagacga
      481 acccagcaag gtgagcgatc caacggagta ggaagttgcg cacagcgtca ctgcacagct
      541 cgcgggagtg tggagcgcat ggcgtgcgca cctcggtaat tcgggtgcca gggcttgttg
      601 tcaccgtcga gccgggggat ggttgggccg gcgatggagc tcgcggatct gctggaggag
      661 cggttcgaga tcgaagaccg gatccaaggt ggcggcatgg gcgaggtgtt tcgcgcccgc
      721 gaccgcgtct ccggcgagcc ggtcgccatc aaggtgatcg ccagcggtca cgatccccgc
      781 ggcgcgcggt tcgcccgcga ggtccggctg ctgtccgagc tctcgcatcc ggcgatcgtg
      841 cgctacgttg cgcacggcgt cacggcgccg ggcgtgccgt tcctggtcat ggagtggctc
      901 gacggcgagg acctccggcg ccggctggag cgcgagccgc tcacgatcgg cgaatcggtc
      961 gcgctcgccg cccgggtcgc cgaggcgctc ggcgtcgccc acgcccgcgg catcgtccat
     1021 cgcgatctca agccgagcaa cctgttcctg cccgaccgcc ggatcgacca ggtcaaggtg
     1081 ctcgacttcg gcatcgcgcg acgggacggg ctgacccacc tgacccggac cgggacgctg
     1141 atcggaacgc ccggctacat ggcgcccgag caggcgcgca gccacgaggt ggtcgacgcg
     1201 cgcgccgacg tgttcgcgct cggctgcgtg ctgttcgagt gcctgaccgg gtcgtagccg
     1261 ttcgagggcg acaccgcgat cgcgatcctc ggcaagatcc tgttcgagga cgcgccccgg
     1321 gtcagcgcgc tgtggcccga ggtgcccgag gatctcgatg cgctggtcgc ccggatgctg
     1381 gcgcgcgacc ccgcgctgcg gccgagcgac ggagcgagcc tcgccgcggc gctggcggcg
     1441 ctcgacccgc gggcccatag ctcgatcgtc ccaccgcgcg ggcagtcggt gcggcgcccg
     1501 gcgctgaccg acggcgagcg gcggctgctg tcggtggtac tgctcgggcc ggcc
//
LOCUS       DASVOR010000702         2842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_328155,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000702 DASVOR010000000
VERSION     DASVOR010000702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328155"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            231..1091
                     /locus_tag="VF516_13885"
     CDS             231..1091
                     /locus_tag="VF516_13885"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8108816.1"
                     /translation="MDRLYLLAPAMLLLATFCLAFAVYCGRCAVGRPPVIHHVKHNQL
                     FGPFLARFLVWLIGPLERLLIGRVSPNAITLASLLLCGITGAAVALGHLPGAVWLYVF
                     AGVLDVLDGRIARQSNQQTPCGALLDSVSDRWGELFVFAGYLWFLQDSPWLVAVMAAI
                     GGSMMVSYTRARAESLGVVLSGGVMQRAERIVLVACGSLIAAWCGADAETSALVDPIL
                     GITMLVCGVGATATAISRWIIAYRELARRHPLRGGPDHAAVLAPPSPPAPARVLIPPH
                     VRKVAPIEQS"
     gene            complement(1123..1890)
                     /locus_tag="VF516_13890"
     CDS             complement(1123..1890)
                     /locus_tag="VF516_13890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEX8108817.1"
                     /translation="MKLATWNINSIRAREERLTTWLGREKPDVLCLQETKVEDAGFPL
                     AALRTAGYEVAMFGQRSYNGVAIASTAPLTDVSRGLGDDPADDEARLIAATTHGMRVV
                     CVYVPNGQDLASDRYPYKLAWFKRLRAFLDRTTSPDQPVALCGDMNVTPDDRDVWSPD
                     RWQNQIHCSPPERAALADVAAFGLTDAFRHHHGDDKLYSWWDYRGVAFFKDQGLRIDL
                     IYLSSPLLARCTACVIDRNARKGQDASDHAPVVAVLA"
     gene            2003..2146
                     /locus_tag="VF516_13895"
     CDS             2003..2146
                     /locus_tag="VF516_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108818.1"
                     /translation="MKAQNDLSLWSIIERLQRRLERQGMTPATADRALSLALRRLAMS
                     PTK"
     gene            complement(2214..2522)
                     /locus_tag="VF516_13900"
     CDS             complement(2214..2522)
                     /locus_tag="VF516_13900"
                     /inference="COORDINATES: protein motif:HMM:NF021188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEX8108819.1"
                     /translation="MEIDFPYELSDQDARGRLEILGRYLANKHGIKVTWVDAAKAKFS
                     GKYLVVRIDGELTLGGGRAKFRGEDPGFLWRGRAKDYIQNKLATYLDPKNAPEHLPTG
                     "
     gene            complement(2559..>2842)
                     /locus_tag="VF516_13905"
     CDS             complement(2559..>2842)
                     /locus_tag="VF516_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108820.1"
                     /translation="VAARFLDIARTDLAQAGQAGIDGATRRAVEDQLPAMRDSLAQAC
                     ADGRWSAAVRNCLVAARDHVGFEACEQQLTDDQRRDLDRANRGAAGSAP"
BASE COUNT          377 a          979 c         1028 g          458 t
ORIGIN      
        1 gacgagccag cgcatggcgg aactatatgc cgaccgcgcg caggcgcgct ctgcgacgcg
       61 cgcgcgcgac gcgcggtcgt tggcgcgcag gtggcgcgca tgaaacaaaa gttcactcaa
      121 aggaccattc gacagcccga aatctcgatg ccaggctttg accttggccg gcctagccgg
      181 gtcgtgataa ccccatctgc ggcgcctcgc gccgcctcct cgaggcccac gtggacaggc
      241 tctacctcct tgcgccggcg atgttgctgc tggcgacgtt ctgcctcgcg ttcgcggtgt
      301 actgcgggcg ctgcgcggtg ggccggcctc cggtgattca ccacgtcaag cacaaccagc
      361 tgttcggtcc gttcctcgcc cggttcctgg tctggctgat cggaccgctg gagcggctgc
      421 tgatcggccg ggtgtcgccc aacgcgatca cgctcgcgtc gctgctgctg tgcgggatca
      481 ccggcgcggc ggtcgcgctc ggccacctgc ccggcgcggt gtggctctac gtgttcgccg
      541 gggtgctcga cgtgctcgac ggacggatcg cgcggcagtc gaaccagcag acgccgtgcg
      601 gcgcgctgct cgactcggtg agcgatcgct ggggcgagct gttcgtgttc gccggctacc
      661 tgtggttcct gcaggactcg ccgtggctgg tcgccgtgat ggccgcgatc ggcggctcga
      721 tgatggtgag ctatacccgg gcgcgcgccg agagcctggg cgtcgtgctg tcgggcggcg
      781 tcatgcagcg cgccgagcgg atcgtgctgg tcgcgtgcgg cagcctgatc gcggcgtggt
      841 gcggcgccga cgccgaaacc tcggcgctgg tcgacccgat cctcgggatc acgatgctgg
      901 tctgcggcgt gggcgcgacg gccacggcga tcagccgctg gatcatcgcc taccgcgagc
      961 tcgcgcggcg ccacccgctg cgcggcgggc ccgaccacgc cgcggtgctc gcaccgccca
     1021 gcccgccggc gccggcgcgc gtgctgatcc cgccgcacgt ccgcaaggtc gcgccgatcg
     1081 agcagagctg acctgaccca gcaggcccgt ttttcaggcc tgctaggcca ggacggcgac
     1141 caccggggca tggtcgctcg cgtcctggcc cttgcgcgcg ttgcgatcga tgacgcacgc
     1201 ggtgcagcgc gcgagcagcg gcgagctcag gtagatcagg tcgatccgca gcccctggtc
     1261 cttgaagaac gcgacgccgc ggtagtccca ccacgagtac agcttgtcgt cgccgtggtg
     1321 gtggcggaac gcgtcggtca ggccgaacgc ggcgacgtcg gcgagcgcgg cgcgctcggg
     1381 cgggctgcag tggatctggt tctgccagcg gtccggcgac cacacgtcgc gatcgtcggg
     1441 ggtgacgttc atgtcgccgc acagcgcgac cggctgatcg ggtgaggtcg tgcggtcgag
     1501 gaaggcgcgc agccgcttga accacgcgag cttgtacggg tagcggtccg acgccaggtc
     1561 ctggccgttg gggacgtaga cgcagaccac gcgcatgccg tgggtggtcg ccgcgatcag
     1621 gcgggcctcg tcgtcggccg ggtcgtcgcc gaggccgcgg ctgacgtcgg tcagcggcgc
     1681 ggtcgaggcg atcgccacgc cgttgtagct gcgctggccg aacatggcga cctcgtaccc
     1741 cgcggtccgg agtgccgcga gggggaaccc ggcgtcctcg accttggtct cctgcaggca
     1801 cagcacgtcg ggcttttccc ggccgagcca cgtggtcagg cgctcctcgc gcgcgcggat
     1861 cgagttgata ttccaggtcg caagcttcac gcagacaacc taccgcacgg cgctgcaatc
     1921 caaccgggag agcggtgtcg ggaatgtatc gggggcggta cgagttgtag accctgagag
     1981 gccgtcgaac gtgaggatcg acatgaaggc acagaacgac ctgtcgctgt ggagcatcat
     2041 cgagcggttg cagcgccgcc tggagcgcca gggcatgacg ccggcgaccg cggatcgcgc
     2101 gctgtcgctc gcgctgcgcc ggctggcgat gtcgccgacc aagtgacccc agcgcgcgcc
     2161 gcgcgccttc acgctcctac ctacgcctcc tggccacggc cctggcggcg cggctacccc
     2221 gtcggcaggt gctcgggggc gttcttcgga tcgaggtagg tggcgagctt gttctggatg
     2281 tagtccttgg cgcggcctcg ccagaggaac cccgggtcct caccgcggaa cttggcgcgg
     2341 ccgccgccga gcgtcagctc gccgtcgatg cggaccacga ggtacttgcc ggagaacttc
     2401 gccttggcgg cgtcgaccca ggtcaccttg atgccgtgct tgttcgcgag gtaccggccg
     2461 aggatctcga gccgcccccg ggcgtcctga tcggacagct cgtacggaaa atcgatctcc
     2521 atccctagat ccgatcgtct accacgtttc gggcgccgct acggcgcaga tccggcggcg
     2581 ccccggttcg cgcggtccag atcacggcgc tggtcgtcgg taagttgttg ctcgcacgcc
     2641 tcgaagccga cgtggtcgcg ggcggccacc aggcaattcc ggacggcggc gctccaccgt
     2701 ccgtcggcgc acgcctgcgc cagggagtct cgcatcgccg gcagctggtc ctcgaccgcg
     2761 cggcgcgtcg cgccgtcgat cccggcctgg ccggcctggg cgaggtcggt gcgcgcgatg
     2821 tccaggaacc ttgcagcgac cg
//
LOCUS       DASVOR010000703         2344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_123537,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000703 DASVOR010000000
VERSION     DASVOR010000703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123537"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1054)
                     /locus_tag="VF516_13910"
     CDS             complement(<1..1054)
                     /locus_tag="VF516_13910"
                     /inference="COORDINATES: protein motif:HMM:NF039196.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8108821.1"
                     /translation="MAESFETKLNGGRASKNGNSRKAKPKTKPRPPGRSGTFSDELQY
                     VLMRFKEGDFASRMPASFVGMEGKVADLLNEILAVSERRATEITRICRVVGKEGKLKQ
                     RMNVPGAVGGWADEIEALNTLIYDLVWPTTEVTRAIGAVAKGDLSQSMALEVDGRRLE
                     GEFLRSAQLVNNMIDQLSVFTSEVTRVAREVGTEGKLGGQAQVKGVSGVWKDLTESVN
                     QMAGNLTAQVRNISEVTIAVASGDLSKKITVDVRGEILQLKEAINTMVEQLRSFASEV
                     TRVAREVGTEGKLGGQAVVPGVAGTWKDLTDNVNSMASNLTGQVRNIAEVTTAVARGD
                     LSRKITVDVKGEILQLK"
     gene            complement(1216..>2344)
                     /locus_tag="VF516_13915"
     CDS             complement(1216..>2344)
                     /locus_tag="VF516_13915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108822.1"
                     /translation="AARRAAEEASRGKDEFVAMLSHELRNPLWAAANALAVLRAARPG
                     LGPELAIAERQIGQLTHLVGDLLDVGRLTRGTIELRREAVQLARVIADAVDDARPRIE
                     RRAHHVAANVSDDLLVDADRHRLRQVVENLLDNAVDHTPDHGHIAISAALDGSDVRIT
                     VRDDGRGIPAALLPSVFELFVQGEQPLDRSDGRLGVGLTLARTLVELHGGTIEAHSDG
                     PGLGATFAVRWPRASEATPTAEIVPLARPSPVARRRVLIVDDNSDAAEMLAALLETLG
                     YEIALATDGAVALDVARDFAPQIALLDIGLPIMDGYEVARRLRQLDACRDTLLVAISG
                     YGQPGDRERSRQAGFAHHLVKPVELATLTSLLETVQGDPTR"
BASE COUNT          309 a          823 c          836 g          376 t
ORIGIN      
        1 ccttgagctg gaggatctcg cccttgacgt cgacggtgat cttgcggctg aggtcgccgc
       61 gcgcgaccgc ggtcgtcacc tcggcgatgt tgcggacctg gccggtcagg ttactggcca
      121 tcgagttgac gttgtcggtg aggtccttcc aggtgccggc gacgccgggc acgacggcct
      181 ggccgccgag cttgccctcg gtgccgacct cgcgggcgac gcgggtcacc tccgaggcga
      241 acgaccgcag ctgctcgacc atcgtgttga tcgcttcctt gagctgcagg atctcgccgc
      301 ggacgtcgac cgtgatcttc ttggacaggt cgccggacgc cacggcgatc gtcacctcgg
      361 agatgttgcg gacctgcgcg gtcaggttgc cggccatctg gttgaccgac tcggtcagat
      421 ccttccacac gccggacacg cccttgacct gggcctggcc gccgagcttg ccctcggtgc
      481 cgacctcgcg ggcgacgcgg gtcacctccg aggtgaacac cgacagctga tcgatcatgt
      541 tgttgaccag ctgggcggag cgcaggaact cgccttcgag ccgccggccg tcgacctcga
      601 gcgccatcga ctggctgaga tcgcccttgg cgaccgcgcc gatcgcgcgg gtgacctcgg
      661 tggtcggcca gaccaggtcg tagatcagcg tgttcagcgc ctcgatctcg tcggcccagc
      721 cgccgaccgc gcccggcacg ttcatccgct gcttgagctt gccctccttg ccgacgaccc
      781 ggcagatccg ggtgatctcg gtcgcgcgac gctcgctcac cgcgaggatc tcgttgagca
      841 ggtcggctac cttgccctcc ataccgacga agctggccgg catgcgcgac gcgaagtcgc
      901 cctccttgaa ccgcatcagg acatactgca gctcatcgga gaacgtgccg gatcgcccgg
      961 ggggacgcgg cttggtcttc ggcttcgcct tccgggagtt gccgttcttc gacgcgcgtc
     1021 cgccgttgag tttggtttcg aagctctcgg ccatctcttg ctccgtgcgg gctaggacta
     1081 gcgccactgt gccacacgtc ctgagctcgg ggggagcatg gtatcaagcg cacagtcgtc
     1141 actgtgcggc tgcgcgcagc ggggcattgc cgcacacctg atgacagacc gatggtcagg
     1201 cggtaggaaa tcggactacc gcgtggggtc gccctggacg gtctctaata atgacgtcag
     1261 cgtcgcgagc tcgaccggct tgaccaggtg gtgcgcgaag ccggcctggc gggagcgctc
     1321 ccggtcgccg ggctggccgt agccgctgat cgcgaccagc agcgtgtcgc ggcaggcgtc
     1381 cagctggcgc aggcgccgcg cgacctcgta gccgtccatg atcggcaggc cgatgtccag
     1441 cagggcgatc tggggagcga agtcgcgcgc gacgtccagc gcgacggcgc cgtcggtcgc
     1501 gagcgcgatc tcgtacccca gggtctcgag cagcgcggcc agcatctcgg ccgcgtcgga
     1561 gttatcgtcc acgatcagga cccgccggcg cgcgacgggg gacgggcggg cgagcggcac
     1621 gatctcggcg gtcggggtcg cctcggacgc ccggggccac cgcacggcga acgtcgcgcc
     1681 gaggccgggg ccgtcgctgt gggcctcgat cgtcccgccg tggagctcga ccagggtccg
     1741 cgcgagcgtc aggccgaccc cgagccgtcc gtcgctgcgg tccagcggct gctcgccctg
     1801 gacgaacagc tcgaacacgc tgggcaacag cgccgccggg atgccgcggc cgtcgtcgcg
     1861 gaccgtgatc cggacgtccg acccgtcgag cgcggcggag atcgcgatgt gcccgtgatc
     1921 cggcgtgtgg tcgactgcgt tgtcgagcag gttctccacc acctggcgga gccggtggcg
     1981 gtcggcatcg accagcaggt cgtcggagac gttcgccgcg acgtggtgcg cgcggcgctc
     2041 gatccgcggg cgcgcatcgt cgaccgcgtc ggcgatcacg cgggcgagct ggaccgcctc
     2101 gcggcggagc tcgatcgtgc cgcgcgtgag ccgcccgacg tcgagcaggt cgccgaccag
     2161 gtgcgtcagc tggccgatct ggcgctcggc gatcgcgagc tccggaccca gccccggccg
     2221 cgccgcgcgc agcaccgcca gtgcgttggc cgcggcccac agcgggttgc gcagctcgtg
     2281 gctcagcatc gcgacgaact cgtccttgcc ccggctcgcc tcctcggcgg cgcgccgggc
     2341 ggcg
//
LOCUS       DASVOR010000704         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_474449,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000704 DASVOR010000000
VERSION     DASVOR010000704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474449"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF516_13920"
     CDS             <1..210
                     /locus_tag="VF516_13920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108823.1"
                     /translation="GRLTPAARDAGHAAIRNRLGAAQLSASGPIVVRLVDGWLDDLGA
                     ILGAVTDAEIDPRFVEGVLVEVHRS"
     gene            complement(224..2101)
                     /locus_tag="VF516_13925"
     CDS             complement(224..2101)
                     /locus_tag="VF516_13925"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8108824.1"
                     /translation="MKRTVALSLLIASLAPAARAAPEPDPIADRVARMSAVGFCVAPS
                     FSPDGASIAFLSNLTGVPQVFTVPTDGGWPVQVTAGSDPAGAPAWSPKGDLIAYTVSP
                     GGGLNTQVHVVAPDGTGARRLTAGGKDNNQLWVWTDDGAALTLSSNERDAASFDAYLV
                     DARSGRKTLVARIGGTGGVGEVSRDGRRAVLSRTRSRGDSDLFLVDLAGKTEIPLTHH
                     TPPGQSSGQIAPDGRTVYLRTNNNRDRMAFGRIRIGADGKPGAFEVIAERGDAEVDGF
                     ALSHDGAAAAIVWNAAGKSLLALVDLAAARPAITPIAVPDDIVGGVTFSRDGKRLAMA
                     VGGATRPTDVWVLDIASRKTWQVTHSPHPGVALAELQRPELVSFKAHDGLALSGWLYR
                     PPGATGPAPYVVSFHGGPEGQERPAFRSDYQALLGQGIGVFAPNVRGSSGFGKTFVNL
                     DNGERRIESLQDIKASVDFLVGQHIAHPRKIGVMGGSYGGYMTMAALTEFPDLFAAGA
                     DLYGIVNFATFFAHSEPWMAAISTTEYGDPKTQKDLLDRLSPIHKIDRIKAPLIVLHG
                     ANDTNVPVIEAEQAVAALKKRGSPVEYVLFPDEGHGWRKTANRITSTVAITKFFVKYL
                     K"
BASE COUNT          299 a          821 c          891 g          335 t
ORIGIN      
        1 ggccggctca cccccgccgc ccgcgacgcc ggccacgccg cgatccgcaa ccggctcggc
       61 gccgcccagc tgtcggcctc cggcccgatc gtcgtccgcc tggtcgacgg ctggctcgac
      121 gacctcggcg cgatcctcgg cgcggtcacc gacgccgaga tcgacccgcg gttcgtcgag
      181 ggcgtcctcg tcgaggtcca ccgcagctga ccgtcgcggc cggctacttc aggtacttga
      241 cgaagaactt cgtgatcgcg acggtcgagg tgatccggtt ggcggtcttg cgccagccgt
      301 ggccctcgtc ggggaacagc acgtactcga ccggcgagcc gcgcttcttg agcgccgcga
      361 cggcctgctc ggcctcgatc accggcacgt tggtgtcgtt ggcgccgtgc agcacgatca
      421 gcggcgcctt gatccggtcg atcttgtgga tcggcgacag ccggtcgagc aggtccttct
      481 gggtcttcgg atcgccgtac tcggtggtcg agatcgcggc catccacggc tcggagtgcg
      541 cgaagaaggt cgcgaagttg acgatgccgt acaggtcggc gcccgccgcg aacaggtcgg
      601 ggaactcggt gagcgcggcc atggtcatgt agccgccata cgacccgccc atcacgccga
      661 tcttcctggg gtgggcgatg tgctgcccga cgaggaagtc gacgctggcc ttgatgtcct
      721 ggagcgactc gatccggcgc tcgccgttgt cgaggttgac gaaggtcttg ccgaagcccg
      781 acgagccgcg cacgttgggg gcgaacacgc cgatgccctg gccgagcagc gcctggtagt
      841 cgctgcggaa cgccgggcgc tcctggccct cgggtccgcc gtggaagctg acgacgtagg
      901 gggccgggcc ggtggcgccg ggtggccggt acagccagcc cgacagcgcc aggccgtcgt
      961 gggccttgaa gctgaccagc tcggggcgct ggagctccgc gagcgccacg ccggggtgcg
     1021 ggctatgggt cacctgccag gtcttgcggc tggcgatgtc gagcacccag acgtcggtcg
     1081 gccgggtcgc gccgccgacc gccatggcga gccgcttgcc gtcgcgcgag aacgtcacgc
     1141 cgccgacgat gtcatcgggg accgcgatcg gcgtgatcgc ggggcgcgcg gcggcgaggt
     1201 cgacgagcgc gagcaggctc ttgcccgcgg cgttccacac gatcgcggcg gcggcgccgt
     1261 cgtgcgacag cgcgaagccg tcgacctcgg cgtcgccgcg ctcggcgatc acctcgaacg
     1321 cgcccggctt gccgtcggcg ccgatccgga tccggccgaa cgccatgcga tcgcggttgt
     1381 tgttggtgcg gaggtagacg gtccgaccat ccggggcgat ctgtccggac gactggccgg
     1441 ggggcgtgtg gtgagtcagc gggatctcgg tcttgccggc gaggtcgacg aggaacaggt
     1501 cgctgtcgcc gcggctgcgc gtccgggaca ggaccgcgcg gcggccgtcg cggctgacct
     1561 cgccgacgcc gccggtgccg ccgatccgcg cgaccagggt cttgcgcccc gacctggcgt
     1621 cgacgaggta ggcgtcgaag gacgccgcat cgcgctcgtt ggacgacagc gtgagcgcgg
     1681 cgccgtcgtc ggtccacacc cagagctggt tgttgtcctt gccgcccgcg gtcagccgcc
     1741 gcgcgccggt gccgtcgggc gcgacgacgt ggacctgggt gttgaggccg ccacccggcg
     1801 agaccgtgta cgcgatcagg tcgcccttcg gcgaccacgc gggcgcgccg gccggatcgc
     1861 tgcccgcggt gacctggacc ggccagccgc cgtcggtcgg gacggtgaac acctggggca
     1921 cgccggtcag gttggacagg aacgcgatcg acgcgccgtc cggggagaac gacggcgcga
     1981 cgcagaaccc cacggcggac atccgcgcga cccggtcggc gatcggatcg ggctcgggcg
     2041 ccgcacgggc cgccggggcg agggacgcga tcagcagcga cagcgcgacg gttcgcttca
     2101 tggcgccgac tatcgtgcga ggcgccacgc ctcgcaaccc gatcccggtg tgcgcctgat
     2161 ggcaccggat gcgaggcccg ggccgggtgt gctaacgaca tggccatgtc tcccgcgaca
     2221 ccgccagcct accgctcggg gatggcgacc ggtctcggtg tggtgctgct cgccggggcg
     2281 gcgctcggcg tggccgatgt cgtgcacacc ggcggcggcg cggggccggc ggcgccgctg
     2341 ctcgcg
//
LOCUS       DASVOR010000705         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_474454,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000705 DASVOR010000000
VERSION     DASVOR010000705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474454"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2827)
                     /locus_tag="VF516_13930"
     CDS             complement(<1..2827)
                     /locus_tag="VF516_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8108825.1"
                     /translation="MADPLAPFGAATQTWFRQSFAEPTPVQARGWASIAAGRHTLMLA
                     PTGSGKTLAAFLWCLDRLARAPRGEPGTRVVYVSPIKALAYDVERNLRAPLAGLVRLG
                     AGGEITVDVRTGDTPAKERERQRRQPADILITTPESLYLVLAGRSRERLRAVDTIIVD
                     EIHALAPTKRGIHLGLSLERLCHLVTGAGGAEPQRIGLSATQRPLAEVARYLGGDRPV
                     EVVDASAPPAMDLAIEVPAAEMDHPGTAARDGGGMWPLIYPRLLALILAHRTTIIFVN
                     SRRLAERVSQQLTELAASTGAAPEGAELVRAHHGSVARHQRLEIEEALKAGRLRAIAA
                     TSSLELGIDMGTVDLVIQVESPGAVSRGLQRIGRAGHHVGGVSVGRIVPKFRGDLLEA
                     TVVADRMRAGEVEAIRVPDSALDVLAQQIVAMVAADAWRVDELERLIHRTASYRDLSR
                     AALTGVLDMLAGRYPSDEFAELRPRLTWDRQTDVLVGRKGARLLSVVSGGTIPDRGTY
                     AVHAGASGPRVGELDEEMVAESRKGETFLLGATTWRIEDITRDRVIVSPAPGEPGKMP
                     FWRGEGPGRPLELGRALGAFCRELDGRLARDPAGAHGWLTGAHRLDRFAADNLIAYLG
                     EQRAATGGVPTDRAITIERFRDELGDVRVCVLSPFGSRVHAPWSLVLARQLEAELGYP
                     IHPLYTDDGIALRFADGDALPTDDQLIPDPDDVEAVLVDELARSSVFASHFRENAARA
                     LLLPRRRPGQRTPLWAQRLRAQQLMGVALRFPAFPITLETYREVLRDVFDLPALVEVL
                     RQIRSRAIRIEPAVTETASPFARSLVFDYVAAFLYEGDAPLAERKAQALTLDRNMLAE
                     LIGGGELRELLDLEVMAEVEAELQQRAPERRARSADEIHDLLRRVGDLSTDEIAERAD
                     PEVLAGGLAGGLVAAIDALAASRRIAAV"
     gene            3115..>3405
                     /locus_tag="VF516_13935"
     CDS             3115..>3405
                     /locus_tag="VF516_13935"
                     /inference="COORDINATES: protein motif:HMM:NF020892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8108826.1"
                     /translation="MTTSLLTTARPDVHARPHLGFAYELVLADALARHRRRRGRDVRF
                     VTGSDDNSLKNVAAAAREGVAPHALVERNTAAFRALGPLLDVAPDDFVRTSVD"
BASE COUNT          437 a         1367 c         1179 g          422 t
ORIGIN      
        1 ggaccgccgc gatccgccgg ctcgcggcca gcgcgtcgat cgcggccacg agccccccgg
       61 ctagcccgcc ggcgagcacc tcgggatccg cgcgctcggc gatctcgtcg gtcgacaggt
      121 cgccgacccg gcgcaggagg tcgtggatct cgtcggccga acgagccctc cgctccggcg
      181 cgcgctgctg cagctcggcc tcgacctcgg ccatcacctc gagatcgagc agctcgcgca
      241 gctcgccgcc gccgatcagc tcggccagca tattccggtc gagcgtcagc gcctgcgcct
      301 tgcgctcggc gagcggcgcg tcgccctcgt agaggaacgc cgcgacgtag tcgaacacca
      361 gcgagcgggc gaacggcgac gcggtctcgg tcaccgccgg ctcgatccgg atcgcgcgcg
      421 agcggatctg gcgcagcacc tcgaccagcg ccggcaggtc gaacacgtcg cgcagcacct
      481 cgcggtaggt ctccagcgtg atcgggaacg cgggaaaccg cagcgccacc cccatcagct
      541 gctgcgcgcg cagccgctgc gcccacagcg gcgtgcgctg gcccggccgg cgccgcggca
      601 agagcagcgc gcgcgccgcg ttctcccgga agtggctggc gaacacgctc gaccgcgcca
      661 gctcgtcgac cagcacggcc tcgacgtcgt cgggatccgg gatcagctgg tcgtcggtcg
      721 gcagcgcgtc gccgtcggcg aaccgcagcg cgatcccgtc gtcggtgtac agcgggtgga
      781 tcgggtagcc gagctcggcc tcgagctggc gcgccagcac cagcgaccac ggcgcgtgga
      841 cccggctgcc gaacggcgac agcacgcaga cccggacgtc gccgagctcg tcgcggaacc
      901 gctcgatcgt gatcgcgcga tcggtcggca ccccgccggt cgccgcgcgc tgctcgccga
      961 ggtacgcgat caggttgtcg gccgcgaacc gatcgagccg gtgcgccccg gtcagccagc
     1021 cgtgcgcccc ggccggatcg cgcgccagcc ggccgtcgag ctcgcggcag aacgcgccga
     1081 gcgcccggcc gagctcgagc ggccgccccg ggccctcgcc gcgccagaac ggcatcttgc
     1141 ccggctcgcc cggcgccggc gacacgatca cccggtcgcg cgtgatgtcc tcgatccgcc
     1201 aggtcgtcgc gccgagcagg aaggtctcgc ccttgcggct ctcggccacc atctcctcgt
     1261 cgagctcgcc gacccgcggg ccagaggccc cggcgtgcac cgcgtacgtc ccgcgatccg
     1321 ggatcgtccc gcccgacacc acgctgagca gccgcgcgcc cttgcggccg accagcacgt
     1381 cggtctggcg atcccaggtc agccgcggcc ggagctcggc gaactcgtcg ctcgggtagc
     1441 gcccggccag catgtcgagc accccggtca gcgcggcgcg cgacaggtcg cggtagctcg
     1501 cggtgcggtg gatcagccgc tcgagctcgt cgacccgcca cgcgtcggcc gccaccatcg
     1561 cgacgatctg ctgggccagg acatccagcg cgctgtccgg cacccggatc gcctcgacct
     1621 cgccggcgcg catccggtcc gccaccaccg tcgcctccag caggtcgccg cggaacttcg
     1681 gcacgatccg cccgacgctg accccgccga cgtggtggcc ggcgcggccg atccgctgca
     1741 gcccgcgcga caccgcgccc ggcgactcga cctggatcac caggtcgacc gtgcccatgt
     1801 cgatgccgag ctcgagcgac gacgtcgcgg cgatcgcgcg cagccgcccg gccttgagcg
     1861 cctcctcgat ctccagccgc tggtggcgcg ccaccgagcc gtggtgcgcg cggaccagct
     1921 cggcaccttc gggcgccgcg ccggtcgacg ccgcgagctc ggtcagctgc tggctcaccc
     1981 gctccgccag ccgccgcgag ttgacgaaga tgatcgtcgt ccggtgggcg aggatcagcg
     2041 cgagcagccg cgggtagatc agcggccaca tcccgccgcc gtcgcgcgcg gccgtccccg
     2101 ggtggtccat ctcggccgcc ggcacctcga tcgccaggtc catcgccggc ggcgcgctgg
     2161 cgtcgaccac ctcgaccggc cggtcgccgc ccaggtagcg cgcgacctcg gcgagcggcc
     2221 gctgggtcgc cgacagcccg atccgctgcg gctccgcgcc gcccgcgccg gtcaccagat
     2281 ggcacagccg ctcgagcgac agcccgaggt ggatcccgcg cttggtcggc gccagcgcgt
     2341 ggatctcgtc gacgatgatc gtgtcgaccg cgcgcagccg ctcgcgcgac cgccccgcga
     2401 gcacgaggta cagcgactcc ggcgtcgtga tcaggatgtc cgccggttgc ctgcgctggc
     2461 gctcgcgctc cttggccggc gtgtctcccg tccgcacgtc gaccgtgatc tcgccgcccg
     2521 cgccgagccg gaccagcccg gccagcggcg cgcgcaggtt gcgctcgacg tcgtacgcca
     2581 gcgccttgat cggcgacacg tacaccaccc gtgtgcccgg ctcaccgcgc ggcgcgcgcg
     2641 ccagccggtc caggcaccac aggaacgccg ccagcgtctt gccgctgccg gtcggcgcca
     2701 gcatcagcgt gtgccggccc gccgcgatgc tcgcccagcc gcgcgcctgc accggcgtcg
     2761 gctcggcgaa gctctgccgg aaccaggtct gggtcgcagc cccgaacggc gccagcggat
     2821 cggccacggc ggcgaccata gcaagccggg cggcgcggca cgtgggcggc cggaggatcg
     2881 gagctgtgcg cgagcatcgg caagaagctg cagctcccgg tctcagactt gcctggaccc
     2941 gcgatgtgct acggtggttt cgtgcgccgc acgtctcgaa tgcagatgcc gatgtccctc
     3001 gaaggacctc gggcatccgt gcgcgctgtt tgacccaacg tgccgagcgg ctgccctcgt
     3061 ggcggctgca cggcttcgtc cgagggcggc ctccggccca gctggaggat cgccatgacc
     3121 acgtcactcc tcaccaccgc caggcccgac gtccacgccc ggccgcacct cggcttcgcc
     3181 tacgagctgg tgctcgccga cgcgctcgcc cgccaccggc gccgccgcgg gcgcgacgtc
     3241 cggttcgtca ccggcagcga cgacaacagc ctgaagaacg tcgccgccgc cgcccgcgaa
     3301 ggcgtcgcgc cgcacgcgct ggtcgagcgc aacaccgcgg cgttccgcgc gctcggcccg
     3361 ctgctcgatg tcgcaccgga cgacttcgtc cggacctcgg tcgat
//
LOCUS       DASVOR010000706         4332 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_562346,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000706 DASVOR010000000
VERSION     DASVOR010000706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4332)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4332)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4332
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_562346"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF516_13940"
     CDS             complement(<1..236)
                     /locus_tag="VF516_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108827.1"
                     /translation="MHDLAEGLALHWLGLAHFHGHGVEKDLARARALQLEAAARGVVD
                     AQLELSLLLAQGIGGRRDARGAARWEAKAAEAGHP"
     gene            complement(329..1030)
                     /locus_tag="VF516_13945"
     CDS             complement(329..1030)
                     /locus_tag="VF516_13945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108828.1"
                     /translation="MSAGGEAFVAALGRPDPAGPPGLSFPNPVDQETWDYLYPEVGAG
                     YYLNRFLYLFGANLSSLEPCLQAWSFLLPTDGARVILGRNAYGALLILENANLSGTKS
                     RVYVLDPIGVVYRRNPQAALENLFGYWLPNKALPNFFNSGIYDTWVAGSGRYLADDEI
                     LGIKVPLGLGGSLALDNFQPEPIVRYYQTTGPIYAKAFAQMAASAGRPPADEDTGKKT
                     EKPGRGKRGGKRGKT"
     gene            complement(1027..>4332)
                     /locus_tag="VF516_13950"
     CDS             complement(1027..>4332)
                     /locus_tag="VF516_13950"
                     /inference="COORDINATES: protein motif:HMM:NF026942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 15 domain-containing
                     protein"
                     /protein_id="HEX8108829.1"
                     /translation="LIDAMAKAADLMNWAGLSRLTFPSFQNKFEAGVIAVSAENFGFT
                     VGGFLQGGGSLALDNARISFDGSAKVTIPGGSEGELRIKQDPTGALTGSLNILVQIGS
                     VAGTVVATLSRGFVSIMGQVGYSGDRLSGKVTLVATDEATARDITLAKPSAGGDVPIE
                     LPGPDKPARPGKRAFCGWGQLTFRVTDWLAGAATVIVNSRGQATIIGEIAPPKEFILF
                     DQKEWIKKILKLEIRAGYGIPVVGQVGIFASIGLDAIAKVGPGKLYNIKLSGAYSTDP
                     RVARNLAIEGTLNISAFAGLRLRAEAGLVVTILGHDIKAGVGLNAIAGVRGYVEATPR
                     IGMRELAPGGKREYFLQGHLEIAAQPVLGFSGDLFVAIETPWWSPLSDKRWTWPLFSI
                     EYPLPGEFGIGADVDYVLGSRQWPKIAFGEVNFDASKFMTDVMNDNTDSGSGGEQKKQ
                     GEWQEGLGAAGKGGAKNKGGAGKKPGELDEDGPIGEEETFSDGKETHRLWFEQKGVNT
                     TLMMASGRPTGVQGKLAEFDHSIDLLGEDKQNHARSLLGKVKEQLAGLDTEADQLAAM
                     RLAARRAKEVYAKAKDRSRKKGSKGKDGLRELAKKVTADQRKVRDPLSQLAFLILEIP
                     FKPGAATAAMDGGTQALRTVEDGGGAAVNADGKEIRKFLADISPGGPIIKAKNPAGVQ
                     AVKLAREQVLPLATDLKKIKIRGGRINRLQADRLVQLFGKVEKVISKLGQKLLRIRNL
                     HRALKIDPIAQKNISFIFYRLPVAQHQQNLEAEVARQLKKQQDGMNRLTVDQFLVNAD
                     TYQSDKFEYFDKLRGKAKAAVVAELEERARTAIAGAHGNTEKLSKELTKLQAQLKTVE
                     DQIAAETKAIAEARERTAQAQATRDAAKARRHELEKQRSQVNREFRAATREQGAQGAT
                     LASAQLVETQLAALDLEDANATKATMSSDEVRAFTARYENLEAWRKKHKKALGAISAD
                     KAFLATWDGMQLKGNDFAYAILHNPDQVVGGKGMLDLDAIKVPPTGSSPEHKAEWDRY
                     VQRVKDQIGAHYVNQRLGGFAGEGETREGWPKHVPDLRKHAKIESRTNAAYGIWRMTV
                     KLDPQYEPPVTKK"
BASE COUNT          605 a         1491 c         1398 g          838 t
ORIGIN      
        1 ggatgacccg cctcggcggc cttggcctcc cagcgcgctg cgccgcgcgc gtcgcgccgg
       61 ccgccgatcc cttgcgccag cagcagcgat agctcgagct gggcgtcgac gacaccgcgc
      121 gcggcggcct cgagctgcag cgcgcgcgca cgggcgagat ccttctcgac gccgtggccg
      181 tggaagtgag ccagaccgag ccagtgcagc gcgagcccct cggcgagatc gtgcaccacg
      241 gtgctcgaac gagcgcgcga gcctccgtca ctgccgagaa cgaacggcag cccgcgctga
      301 tagtgcggtg cacgaggccg cctcggggtc acgtcttgcc tcgcttgccg ccgcgcttcc
      361 cgcgaccggg cttttccgtc ttcttgccgg tgtcctcgtc ggcaggtggc cggcccgcag
      421 atgccgccat ctgcgcgaac gccttcgcgt agatcggtcc ggtcgtctgg tagtagcgaa
      481 cgatcggctc tggctggaag ttgtccagcg cgagactgcc gcccaggccg agtggaacct
      541 tgatcccgag aatctcgtcg tcagcgagat atcggccgct gccggcgacc catgtgtcat
      601 agatgcccga gttgaagaag ttcggcagcg ccttgttcgg aagccagtag ccaaacaggt
      661 tctcaagcgc agcttgtggg tttcgtcggt agacgacgcc gatggggtcg agcacgtaca
      721 cgcgcgactt cgtcccggaa agattcgcat tttcgaggat gaggagcgcc ccgtaggcat
      781 tccggccgag gatcacgcgc gcgccgtcgg taggcagcag gaacgaccaa gcctgcagac
      841 acggctcaag cgagctcagg ttagctccga acaggtacag gaaccggttc aagtagtagc
      901 cggcgccaac ctccgggtac agatagtccc aggtctcctg gtcgaccggg ttcgggaaag
      961 acagaccggg cggccccgca ggatccggcc gccccagcgc cgcgacgaac gcctctccgc
     1021 cggcgctcat ttcttcgtca ccggtggctc gtactgcgga tccagcttca cggtcatcct
     1081 ccagatgccg tacgcggcgt tggtgcgcga ctcgatcttc gcatgttttc tgaggtcggg
     1141 gacatgcttc ggccacccct ctctcgtctc gccctcaccc gcaaagccac ccagccgctg
     1201 gttcacatag tgagcgccga tctggtcctt cacccgttgc acgtagcggt cccactcggc
     1261 cttgtgctcc ggtgatgagc cggtcggagg cactttgatg gcgtcgaggt cgagcattcc
     1321 ctttccaccg acgacctggt cgggattgtg aaggatcgcg taggcgaagt cgttgccctt
     1381 cagctgcatt ccgtcccacg tggcaagaaa ggccttgtcg gcggagatcg ccccgagcgc
     1441 cttcttgtgt ttcttgcgcc aggcctcgag gttctcgtag cgggcggtga acgcacgcac
     1501 ctcgtcgctc gacatcgtcg ccttggtcgc gttcgcatcc tcgaggtcga gcgctgccag
     1561 ttgcgtctcg accagctgcg cgctggcgag tgtcgcgccc tgtgcgcctt gctctctggt
     1621 tgccgcgcga aactctcggt tgacctggct gcgttgcttc tccagctcgt ggcgccgcgc
     1681 cttggccgcg tcgcgcgtcg cctgggcctg cgccgttcgc tctcgcgctt ccgcgatcgc
     1741 cttcgtctcg gccgcgatct ggtcttcgac ggtcttgagc tgcgcttgca gcttggtgag
     1801 ctctttcgag agcttctccg tgttaccgtg cgcgccggcg atggcagtcc tggcccgctc
     1861 ttcgagctcg gcgacgacgg cggccttcgc cttgccccgc agcttgtcga agtattcgaa
     1921 cttgtccgac tggtacgtgt cggcattgac gagaaactgg tcgacggtga gacggttcat
     1981 cccgtcctgc tgcttcttga gctggcgggc cacctcggcc tcgagattct gttgatgctg
     2041 cgcgaccggc aaccggtaga agatgaaact gatgttcttc tgcgcgatcg ggtcgatctt
     2101 caacgcgcga tgcaggttcc tgatcctcag cagcttctgt cctagcttgc tgatgacctt
     2161 ctcgaccttg ccgaacagct gcacgagacg gtcggcctgc agccggttga tccttccgcc
     2221 tcggatcttg atcttcttga gatcggtcgc gagcggcagc acctgctccc gtgccagctt
     2281 cacggcctgg actcccgccg gattcttcgc cttgatgatc ggtccccccg gggagatgtc
     2341 tgccaggaac ttccggatct ccttgccatc cgcgttaacc gccgcgccgc cgccgtcctc
     2401 gacggtccgc agcgcctgag tgccgccatc catcgccgcc gtcgcggcgc ccggcttgaa
     2461 agggatctcc aggatgagga acgcgagctg gctcagcgga tcgcgcacct ttcgctgatc
     2521 ggcagtgacc ttcttagcca gctcgcgcag gccatccttc cctttgctgc ccttcttgcg
     2581 agatcggtcc ttggccttcg cgtagacctc tttcgcacgc cgggcggcca gcctcatcgc
     2641 cgccagctgg tcggcctccg tatcgagccc cgcgagttgc tccttcacct tgccgagcag
     2701 gctccgcgcg tggttctgct tgtcctcacc gagaagatcg atcgagtggt cgaattctgc
     2761 cagctttcct tggacgcccg tcgggcgccc gctggccatc atcagcgtgg tgttcacacc
     2821 cttctgctcg aaccacagcc gatgcgtctc tttgccgtcg ctgaacgtct cctcttcgcc
     2881 gatcggcccg tcctcgtcga gctcgcccgg cttcttgccc gcgccgccct tgttcttggc
     2941 gccgcccttg cccgcggcgc cgaggccctc ctgccactcg ccctgcttct tctgctcgcc
     3001 gccgctgccg ctgtcggtgt tgtcgttcat gacgtcggtc atgaacttcg acgcgtcgaa
     3061 gttgacctcg ccgaacgcga tcttcggcca ctgcctggag ccgagcacgt agtcgacgtc
     3121 ggcgccgatg ccgaactcgc cgggcagcgg gtactcgatg ctgaacagcg gccaggtcca
     3181 gcgcttgtcg gacagcggcg accaccacgg ggtctcgatc gcgacgaaca ggtcgcccga
     3241 gaacccgagc accggctgcg cggcgatctc gaggtggccc tggaggaagt actcgcgctt
     3301 gccgccgggg gcgagctcgc gcatgccgat gcgcggcgtg gcctcgacgt agccgcgcac
     3361 gccggcgatc gcgttgagcc cgacgccggc cttgatgtcg tggccgagga tggtgacgac
     3421 gagcccggcc tcggcgcgca gccgcaggcc ggcgaacgcg gagatgttga gcgtgccctc
     3481 gatcgcgagg ttcctggcga cgcgcggatc ggtcgagtac gcgccggaca gcttgatgtt
     3541 gtacagcttg cccgggccga ccttggcgat ggcgtcgagg ccgatgctgg cgaagatgcc
     3601 gacctgcccg acgacgggga tgccgtatcc ggcccggatc tcgagcttga ggatcttctt
     3661 gatccactcc ttctggtcga agaggatgaa ctccttgggc ggtgcgatct cgccgatgat
     3721 cgtggcctgg cccctggaat tgacgatcac ggtggccgcg ccggcgagcc agtcggtgac
     3781 ccggaacgtg agctgccccc agccgcagaa cgcgcgcttg ccgggcctgg cgggcttgtc
     3841 cggcccgggc agctcgatcg gaacgtcgcc gcccgcgctc ggcttggcga gggtgatgtc
     3901 gcgggcggtg gcctcgtcgg tggcgaccag cgtgaccttg ccgctcagcc ggtcgccgct
     3961 gtagccgacc tggcccatga tgctgacgaa gccgcgcgac agcgtcgcga ccacggtgcc
     4021 cgcgacgctg ccgatctgga ccaggatgtt gagcgagccg gtcaacgccc cggtcggatc
     4081 ctgcttgatc cgcagctcgc cctccgagcc gccggggatc gtgaccttgg ccgagccgtc
     4141 gaacgagatc cgggcgttgt cgagcgcgag gctgccgccg ccctgcagga agcctcccac
     4201 ggtgaacccg aagttctcgg cgctgaccgc gatcacgccg gcctcgaact tgttctggaa
     4261 cgacgggaag gtgagccggg acaggcccgc ccagttcatg aggtcggcgg ccttggccat
     4321 cgcgtcgatc ag
//
LOCUS       DASVOR010000707         5057 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_386519,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000707 DASVOR010000000
VERSION     DASVOR010000707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5057
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386519"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VF516_13955"
     CDS             complement(<1..1013)
                     /locus_tag="VF516_13955"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8108830.1"
                     /translation="MRSALVDLSRVRHLPDWKSWQALTAQGAPFLTPEFFTLASRLNG
                     GDGLVAEAWDGDRLVGALPVVRHGNQLTALACDYTPGYDYCGAPDGIEAIWRALREDR
                     GWNQLVFGKLPIDSPLATRLPTLANAEGCPVAIHLDSRHPYFALPGFEAAMSPKFLSN
                     LQRCARKAGGVELERIASADRAAFDEALAIEAMAWKGIAGTSIATDPRAQHFYRTLIR
                     LFGRRGRGTLYFLRAGGRRIATLFALEDGHTLYALKIGYDPAAANLSPGHLLVWKVAA
                     EAAQRGLQELDFVGREDDWKRKWTSRSHETVAVTLYRDSAAGLARYAAHLARPHLPGA
                     LRGG"
     gene            complement(1155..2198)
                     /locus_tag="VF516_13960"
     CDS             complement(1155..2198)
                     /locus_tag="VF516_13960"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8108831.1"
                     /translation="MAASVSVIVPTFNRARWLGNAIRSALQQLQAGDELIVIDDGSTD
                     DTREVVAAFGERVTYVRGEHRGAGPARNLGLARARGDLVAFLDDDDEWLPGKLARQRA
                     FLDAHPDVLFCFSDFQVVTGDGEVQHRFLRHWRHDARSFRDMFGPPERIAGCDVYVGD
                     LYVWQLTGLYVLANTLVVRRAAAGDALHFAEDLQTYEDVECFIRLSRQGKAAYFDLET
                     AQQTDRAAGRLSQLSLITKLRCHLAILERQYGRDAEFLATHRLVYERTIRQMQAQLLR
                     LQILRGDLAGARHTLAALPHAPLHLWLVLHLPDPWIGRALEGYRRVHTTAGRWLQEPS
                     GSRARHAPSHSPP"
     gene            2565..3758
                     /locus_tag="VF516_13965"
     CDS             2565..3758
                     /locus_tag="VF516_13965"
                     /inference="COORDINATES: protein motif:HMM:NF013631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLAT/LH2 domain-containing protein"
                     /protein_id="HEX8108832.1"
                     /translation="MADQRPPVPGRSFGPEPLWILGHNTNSIEAVNAALDQGANAVEI
                     DVTAYAHDLNALCIDHAGLMGDDPGRASAPAFESFLRELREVVDRRPELCMVEFDLKP
                     PASRPELGPVLSGAIREFLTRGTTLSVILSVADVTTSHADRPRGSSVFDRINGDVRAR
                     EGFMIDSDHDIDGVAEFFLNKQEVARFGYGNGTAVPLVDEGMMVYRTPIEKACWMRAV
                     RGTPGFVEAWTVNSVDNLKLYLHLGVNGLICDPEGIERVRDLLQHGELARRYRLAERS
                     DDPMVPDNFAYGLTVVTTDKRLAGTDASVAFTLSGDSGSASTTVDASLHRRLESGSTH
                     YFVLYSADLGNLRSITVQSDRSHLAPGWHLHSIVVESSRYGGKKTAGFDTWIDSADPV
                     TRELG"
     gene            3997..4923
                     /locus_tag="VF516_13970"
     CDS             3997..4923
                     /locus_tag="VF516_13970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108833.1"
                     /translation="MLPLCLVTLCLATASHLAGCAASDPAPEVSDPELTPDELAALPD
                     AAAPDHDGLADAIAAEPPETDAATFDTDEATEQTDVAVEDTDETAASLIQPQAALLKF
                     GLHPRASDALRSIGITASRILQTIGNAPASAGTHAQDGVVNGHPYSAATDLSVSGLSN
                     AQIKNLLARLAKVGFAAWFRWPGHDGWPASEVRHIHAVYANAKMKAALRSQVRSWLVG
                     RNGLVSNTLYSFFQWTAAEKAVVRNKFAQSGEGTTNAGASCFVGGLYCGGDKIGGDPG
                     TLFRCTGTGAPTVVRHCANGCAVHPGSDDACR"
BASE COUNT          780 a         1735 c         1793 g          749 t
ORIGIN      
        1 ccaccgcgca gcgccccggg caggtgggga cgcgccaggt gcgcggcgta gcgcgcgagg
       61 cccgcggcgc tgtcgcgata cagcgtgacc gcgacggtct cgtgcgatcg gctggtccac
      121 tttcgcttcc agtcgtcctc acgtccgacg aagtcgagct cctgcaagcc gcgctgtgcg
      181 gcctcggcgg cgaccttcca cacgagcagg tggccggggc tgaggttcgc ggcggccggg
      241 tcgtagccga tcttgagcgc gtagagcgtg tggccgtcct ccagcgcgaa cagcgtcgcg
      301 atccggcgac ctccggcacg caggaagtac agcgtgcccc gcccgcggcg cccgaacagc
      361 cggatcagcg tccggtagaa gtgctgggcg cgtggatcgg tggcgatgct ggtcccggcg
      421 atgcccttcc aggccatggc ctcgatcgcg agcgcctcgt cgaacgccgc gcggtcggcg
      481 gaggcgatgc gctcgagctc gacgccgccg gccttgcgcg cgcagcgctg caggttggac
      541 aggaacttcg gcgacatcgc cgcttcgaac ccgggcagcg cgaagtacgg atgacgtgaa
      601 tcgagatgga tcgcgaccgg gcacccctcg gcattggcca gcgtcggcaa gcgggtcgcg
      661 agcggcgaat cgatcgggag cttgccgaac acgagctggt tccagccgcg gtcctcgcgc
      721 agcgcgcgcc agatcgcctc gatcccgtcg ggcgctccgc agtagtcgta ccccggggtg
      781 tagtcgcagg cgagcgcagt gagctggttg ccgtggcgga ccaccggcag cgcgccgacc
      841 aggcggtcgc cgtcccaggc ctcggcgacg agcccgtcgc cgccgttgag acgtgacgcg
      901 agcgtgaaga actcgggggt caggaacgga gctccctggg ccgtgagcgc ttgccagctc
      961 ttccagtccg gaaggtggcg gacgcgagat agatcgacga gggcggagcg catgctccgt
     1021 cgatcagcaa gcgacattcc agggtgctgt ggcgctggcc ggcaccgagc gcgcatcgat
     1081 cgcgccggcc gtcgctgctg gcgcagcaga tgcgccgcgg gcccggcgtg cggcggtgat
     1141 cccgcgcacg gcggtcacgg tggcgagtgg gagggcgcgt ggcgagcacg agagccagac
     1201 ggctcctgga gccatcgccc ggccgtggtg tggacgcgcc gatagccctc gagcgcccga
     1261 ccgatccatg ggtccggcag gtgcagcacc agccagaggt gaagcggcgc gtgcggcagc
     1321 gctgccagcg tgtgccgcgc acccgccagg tcgccgcgca agatctgcag ccgcaggagc
     1381 tgcgcctgca tctgccggat cgtgcgctcg tacaccaggc gatgcgtcgc gaggaactcg
     1441 gcgtcgcggc cgtactgccg ttcgaggatc gcgaggtgac agcgcagctt ggtgatcagc
     1501 gacagctgcg agagccgacc ggcggcgcga tcggtctgct gcgcggtctc gaggtcgaag
     1561 tacgccgctt tcccctggcg cgacagccgg atgaagcact cgacgtcctc gtaggtctgc
     1621 agatcctcgg cgaagtgaag cgcgtccccg gcggcggcac gccggaccac cagcgtgttc
     1681 gcgaggacgt acaggccggt cagctgccag acgtacaggt cgccgacgta gacgtcgcag
     1741 ccggcgatgc gctccggggg cccaaacatg tcgcgaaacg atcgcgcgtc gtgccgccag
     1801 tgccgcagga agcggtgctg gacctcgccg tcgccggtca ccacctggaa gtcggagaag
     1861 cagaacagca cgtccggatg ggcgtcgagg aatgcgcgct gcctcgcgag cttgccgggc
     1921 agccattcgt cgtcgtcgtc gaggaaggcg accaggtcgc cgcgggcgcg cgcgaggccc
     1981 aggttgcgcg ccgggccggc gccgcggtgc tcgccgcgca cgtaggtcac gcgctcgccg
     2041 aacgcggcga ccacctcgcg ggtgtcatcg gtcgatccgt cgtcgatcac gatcagctca
     2101 tcgccggctt gcagctgctg cagcgccgag cggatcgcgt tgcccagcca gcgggcgcgg
     2161 ttgaaggtcg gcacgatgac agagaccgag gcggccatgg catccctcct atgcgagtgg
     2221 ctcggcacct tcgcgctcgt gcctggccgg ctccggcggc tcggtcatcg tcgcctcgtt
     2281 acgaaagacg agttgcttgg cgggcatgtc ggcgcatgga gcaacatgca tgccttctcg
     2341 tgatcacgta tcgtgtgctg tcatccgacc aagatgatcg ccggatcgtc ggggccccgt
     2401 agcgcgagca cgggtgtgcc agtggtggat ggggtccgag atcgaggcgg cgcaagcgct
     2461 ccggccactc gcgacatggg cgcaatggat gcgcgatatg cgcgggtgtg acgtcgccgc
     2521 ggacgttgct acactcacgg tcgtcaactg aatcgaggcc tatcgtggca gatcaacgcc
     2581 ccccggtgcc cggtcgttcg ttcggacccg agccactgtg gatcctggga cacaacacga
     2641 attcgatcga ggcggtcaac gccgcgctgg accagggcgc caatgccgtc gagatcgacg
     2701 tcaccgcgta cgcccacgat ctgaacgcgc tgtgtatcga tcacgccggc ctcatgggtg
     2761 acgatccggg gcgcgcgagc gcgccggcct tcgagtcgtt cctgcgcgag ttgcgcgagg
     2821 tggtcgatcg ccgacccgag ctgtgcatgg tggagttcga tctcaagccg cccgcttcgc
     2881 gcccggagct cggtcccgtg ctgtcgggcg ccattcgcga gttcctcacc cgggggacga
     2941 cgctcagcgt gatcctgtcg gtcgcggatg tcaccacgag ccacgccgat cgcccgcgcg
     3001 gcagctcggt gttcgaccgc atcaacggtg atgtgcgtgc ccgcgagggc ttcatgatcg
     3061 actccgacca cgacatcgac ggggtcgcgg agttcttcct gaacaagcag gaggtcgctc
     3121 gcttcgggta tggcaatggc accgcggttc cgctggtcga tgaaggcatg atggtctatc
     3181 gcacgcccat cgagaaggcc tgctggatgc gcgcggtgcg tggcaccccc ggcttcgtgg
     3241 aggcgtggac cgtcaacagc gtcgacaacc tcaagctcta cctgcacctc ggtgtcaacg
     3301 ggctgatctg cgaccccgag ggcatcgagc gcgtgcgaga cctcctccag cacggcgagc
     3361 tcgccaggcg ctatcggctg gccgagcgtt ccgacgatcc gatggtgccg gacaacttcg
     3421 cgtacgggct caccgtcgtg accaccgaca agcgcctcgc ggggaccgat gcgagcgttg
     3481 cgttcacgct gagcggcgac agcggctcgg cctcgaccac ggtggacgcc agcctccacc
     3541 gtcgcctgga gtccggatcg acccattact tcgtgctgta cagcgccgat ctaggcaact
     3601 tgagatccat caccgtgcag agcgatcgga gccacctcgc gcccggctgg caccttcact
     3661 ccatcgtggt cgagagcagc cggtatggtg gcaagaagac cgccggcttc gacacctgga
     3721 tcgactcggc cgatcccgtc acgcgagagc tcgggtgacc gaccgcggca gcttcggtcg
     3781 agcgacgaac agggagcgtc ggacgagatc gccgtcacgg aacggcgcgc gtcaccatcc
     3841 cgctgccact gcctgtccgc atctgtagcg cgcgccgcta cacccggatg gcgattcagc
     3901 tgcagcgtcg cggagatagc gctgggtacg cggcttgccc ttgctcgtcg ccatcacgcc
     3961 ggggtcgctg accccaaagg agaaccgatg actcgcatgc tacctctctg cctcgtcact
     4021 ctgtgcctcg ccacggcgag ccacctcgcc ggctgcgccg cgtccgaccc cgcgcccgag
     4081 gtcagcgatc ccgagttgac acccgacgag ctcgccgcgc tgccggacgc cgccgcgccc
     4141 gatcacgatg ggctcgccga cgcgatcgcg gccgagccgc ccgagaccga cgcggcgacg
     4201 ttcgacaccg acgaggcgac cgagcagacc gacgtcgcgg tcgaggacac cgacgagacc
     4261 gccgcgtcgc tcatccagcc ccaggctgcg ctgctcaagt tcggcctgca tccgcgcgcc
     4321 tcggacgcgc tgcgctcgat cggcatcacc gcgtcgcgca tcctgcagac gatcggcaat
     4381 gcgccggcgt cggccggcac ccatgcccag gacggcgtgg tcaacggcca cccgtacagc
     4441 gcggcgaccg acctcagcgt gagcgggctc agcaatgcgc agatcaagaa cctgctcgcg
     4501 cggctggcca aggtcggctt cgcggcgtgg tttcgctggc ccggccacga tggctggccg
     4561 gcttcggagg ttcgccacat ccacgcggtc tatgccaacg ccaagatgaa ggccgcgctg
     4621 cgctcgcagg tccggtcgtg gctggtcggc cgcaatggcc tggtcagcaa cacgctgtac
     4681 agcttcttcc agtggaccgc agccgagaag gcggtggtgc ggaacaagtt cgcgcagagc
     4741 ggggagggca cgaccaacgc cggcgcgagc tgcttcgtcg gcggtctgta ctgcggcggt
     4801 gacaagatcg gcggcgaccc cggcacgctg ttccgctgca cgggcaccgg cgcgccgacc
     4861 gtggtgcgcc actgcgccaa cggctgcgcg gtgcacccgg gcagcgacga cgcgtgcagg
     4921 tgagcggcgc gtctgctatc gcagcccgac ccgggcgagc aggcggtgga agtggacccg
     4981 gtcgatgccg gcggcgcgcg ccgcggcgct gacgttgttg ccgtgcagca cgagcagctg
     5041 ctcgaggtag cggcgct
//
LOCUS       DASVOR010000708         1883 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_299145,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000708 DASVOR010000000
VERSION     DASVOR010000708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299145"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VF516_13975"
     CDS             <1..357
                     /locus_tag="VF516_13975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108834.1"
                     /translation="PADLVRTGLAISGVFELAPLIPTTMNTKLRLDPEAAADASPLFW
                     PPPPPGRTLVAAVGEHETPEFHRQSRAIVERWASAGVATEYLSVAGVHHFTIVDELAR
                     PGNALFDRVVALTRTP"
     gene            408..1703
                     /locus_tag="VF516_13980"
     CDS             408..1703
                     /locus_tag="VF516_13980"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8108835.1"
                     /translation="MNVRQLVEPDRVHRTVYTDQQIFDRELTQIFERVWVYCGHQTQV
                     PQPGDYHVFTIGRQPMFMVRGKDGQIHVLHNRCPHRGVQLVGNQKGNVGGALVCSYHA
                     WSFQLDGKFRSTPLPHGYDGTRVGAGNPDCSVKPAARVDSYRGFVFASLSPTGPSLLD
                     FLGEAKVAFDDMCDRSPVGEVEVVPICHRVIQHSNWKFFMENQLDALHPSVTHQSTGV
                     AAGRIEKKLKEETGQAPLYYHYLSAFASKFEQWDAVQTINFPHGHGILKAYMGLRPQD
                     PDTRAHEAMLVEAYGPDRTEEYLSRGIHHVLVYPYLSVQSPLQQLRCLRPISPSKTLS
                     EIWHFRLKGAPEAIYRRSLWYYNLVNSPATMVNADDLENWTKGQWGLESEGGDWVSFH
                     RDYGGDTDQNGVIYSNHGTSEAVMRNQFRAWVQYMADTP"
BASE COUNT          309 a          698 c          608 g          268 t
ORIGIN      
        1 ccggcggacc tggtgcgcac cggcctggcg atcagcgggg tgttcgagct cgccccgctg
       61 atcccgacga cgatgaacac caagctgcgg ctcgaccccg aggccgcggc cgacgccagc
      121 ccgctgttct ggccgccgcc gccgcccggc cgcacgctgg tcgccgcggt cggcgagcac
      181 gagacccccg agttccaccg ccagagccgc gcgatcgtcg agcgctgggc gagcgccggc
      241 gtggcgaccg agtacctgtc cgtcgccggc gtccaccact tcacgatcgt cgacgagctg
      301 gcccggcccg gcaacgcgct gttcgaccgc gtcgtggcgc tgaccagaac tccttgaatt
      361 cccgaggagc tttagtaggc ttggtcggtt tccgggaggt gatcgcgatg aatgtccgcc
      421 aactggtcga gccagatcgg gtccaccgca cggtctacac cgatcagcag atcttcgacc
      481 gcgagctgac gcagatcttc gagcgcgtct gggtctactg cggacaccag acccaggtcc
      541 cccagccagg cgactaccac gtgttcacga tcggccgcca gccgatgttc atggtgcgcg
      601 gcaaggacgg ccagatccac gtcctgcaca accgctgccc gcaccgcggc gtgcagctgg
      661 tcggcaacca gaagggcaac gtcggcggcg cgctggtctg ctcgtaccac gcgtggagct
      721 tccagctcga cggcaagttc cgctcgaccc cgctgccgca cggctacgac ggcacgcggg
      781 tcggcgccgg caaccccgac tgcagcgtca agccggccgc gcgcgtcgat tcctaccgcg
      841 gcttcgtgtt cgccagcctg tcgccgaccg gcccgtcgct gctcgacttc ctcggcgagg
      901 ccaaggtcgc gttcgacgac atgtgcgatc gctcgccggt cggcgaggtc gaggtcgtgc
      961 cgatctgcca ccgcgtgatc cagcactcga actggaagtt cttcatggag aaccagctcg
     1021 acgcgctgca cccgtcggtg acccaccagt cgaccggcgt cgccgcgggc cggatcgaga
     1081 agaagctcaa ggaggagacc ggccaggcgc cgctctacta ccactacctg tcggcgttcg
     1141 cgtccaagtt cgagcagtgg gacgccgtgc agaccatcaa cttcccgcac ggccacggca
     1201 tcctcaaggc ctacatgggc ctgcgcccgc aggaccccga cacccgcgcc cacgaggcga
     1261 tgctcgtcga ggcctacggc ccggacagga ccgaggagta cctgtcgcgc ggcattcacc
     1321 acgtcctggt ctatccctac ctctcggtgc agtcgccgct gcagcagctg cgctgcctgc
     1381 ggccgatctc gccgagcaag acgctgtcgg agatctggca cttccggctc aagggcgcgc
     1441 ccgaggcgat ctaccgccgc tcgctctggt actacaacct ggtcaactcg ccggccacga
     1501 tggtcaatgc cgatgatctg gagaactgga ccaagggcca gtggggcctc gagtccgagg
     1561 gcggcgactg ggtgagcttt caccgcgact acggcggcga caccgatcag aacggcgtga
     1621 tctactccaa ccacggcacc tcggaggccg tgatgcgcaa ccagttccgc gcctgggtcc
     1681 agtacatggc ggacaccccc tgagccgaac gaggcgatga ccatggcgag caccccgagc
     1741 aacggcaacc cgcccgcacc ggcgcacccc gacggtaccc cgggcgcgcg cctggcccag
     1801 gacatcgtga tcgagaacgc cggcggcgac gcggtcggcg cgcaggcgat cgcgcccgac
     1861 cacgccgcgc gcgccgcgcc gtc
//
LOCUS       DASVOR010000709         1778 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_386540,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000709 DASVOR010000000
VERSION     DASVOR010000709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1778
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386540"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..584
                     /locus_tag="VF516_13985"
     CDS             96..584
                     /locus_tag="VF516_13985"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HEX8108836.1"
                     /translation="MLGEVIGSHDGKLFRSFAQSLTLDNLLAVANAHLDELAPRYQLE
                     RVPRHDLELQVIDRDLGAEVRSVQSLSGGESFLVSLALALGLSSMSAHDVRVRTLLID
                     EGFGTLDPATLDSALSVFDALQATGRQVGVISHVPALVERVGAHVRVVQRGGGRSEVI
                     VA"
     gene            865..1173
                     /locus_tag="VF516_13990"
     CDS             865..1173
                     /locus_tag="VF516_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108837.1"
                     /translation="MVSILFVGLIGCRAGEGQSANRSDLASNTSTDDPSQVCPYVCVP
                     GILCQMPDGSCTAACNDCLCRRDGGRVVGACPKADSPTGAFQAAASQVVAASDVDRAR
                     "
     gene            1186..>1778
                     /locus_tag="VF516_13995"
     CDS             1186..>1778
                     /locus_tag="VF516_13995"
                     /inference="COORDINATES: protein motif:HMM:NF027439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8108838.1"
                     /translation="MTQHVRVTTADRVTTIRLDRPDKKNAITIEMYDALCAALEAAAA
                     DPAVRAVVSGGTRDCFTAGNDLGDFARAAQGGDTSSGRRFLHTLAGFDKPLIGAVAGV
                     AIGIGTTMLLHCDLVYAAPTARFRTPFVDLALVPEAGSSLLLPALVGARRAAQLLLLG
                     EQLDAQAALAWGLITAVVDDPDAAAAAAAGKLAGLPPG"
BASE COUNT          237 a          649 c          607 g          285 t
ORIGIN      
        1 ccgcttcact cgccgctgat ggcgatgcgc gagcgcgccg cgcgagtgcg caagccgagc
       61 tcgcctctgc ggagcaagcg gccgaagtcg atcgcgtgct gggcgaagtg atcggctcgc
      121 acgacggcaa gctgttccgc agcttcgcgc agagcctgac gctcgacaac ctgctcgcgg
      181 tcgccaacgc ccacctcgac gagctcgccc cgcgctacca gctcgagcgc gtgccgcgcc
      241 acgacctcga gctccaggtc atcgatcgcg atctcggcgc cgaggtccgc tcggtccaga
      301 gcctgtccgg cggcgagagc ttcctggtct cgctcgcgct cgcgctcggt ctgtcgtcga
      361 tgtcggccca cgacgtccgg gtccgcaccc tgctgatcga cgagggcttc ggcacgctcg
      421 acccggccac gctcgacagc gcgctgtcgg tgttcgacgc gctgcaggcc accgggcgcc
      481 aggtcggcgt gatctcgcac gtgccggcgc tggtcgagcg ggtcggtgcg cacgtccgcg
      541 tcgtccagcg cggcggcggg cgcagcgagg tgatcgtcgc ctgacgatca gcgatccgct
      601 gcgccggcgg atgatgcgcg atcggccgat catcgaatgg ctcgctcgct cgccgatggc
      661 tgctggtttt ttgtcgtgca tgcgcgcgca ccgggcgtgt ggagcgtgct catcaggtca
      721 acgacgaccg acacagtctg atgcagtatc tcgccgggtg agagtctgct gatatacctc
      781 agcgcgccgt ggctcgtcgt gaattctggc tcatgacagg agtaggccat ctcaaggaga
      841 atcgatatgc gttccatgct ctccgtggtg tcgatcctgt tcgtggggct gatcggttgc
      901 agggccggcg agggccagag tgccaatcgc tccgatctcg ccagcaacac cagcacggac
      961 gatccgtcac aggtctgccc ctatgtctgc gtgccgggga tcctgtgcca gatgcccgat
     1021 ggcagctgca cggcggcctg caacgactgc ctctgcaggc gcgacggcgg aagggtggtc
     1081 ggcgcgtgcc ccaaggccga ctcgccgacg ggcgcgttcc aggccgccgc gtcgcaggtc
     1141 gtggcggcca gcgacgtcga ccgcgcgcgg tgatatgagt accgcatgac ccagcacgtc
     1201 cgcgtcacca ccgccgatcg cgtcaccacg atccggctcg atcgacccga caagaagaac
     1261 gcgatcacga tcgagatgta cgatgcgctc tgcgccgcgc tcgaggccgc cgcggccgac
     1321 cccgcggtcc gcgccgtcgt gagcggcggc acgcgcgact gcttcaccgc tggcaacgac
     1381 ctcggcgact tcgcgcgtgc ggcgcagggc ggcgacacct cgtccgggcg ccgcttcctg
     1441 cacacgctcg ccgggttcga caagcccttg atcggcgcgg tcgccggcgt cgcgatcggc
     1501 atcggtacca cgatgctgct ccactgcgac ctggtctatg ccgcgccgac cgcccggttc
     1561 cgcacgccgt tcgtcgacct cgcgctggtg cccgaggccg gctcgtcgct tctgctgccg
     1621 gctctggtcg gtgcgcggcg cgccgcccag ctgctgctgc tcggcgagca gctcgacgcc
     1681 caggccgcac tggcctgggg cctgatcacc gcggtcgtcg acgaccccga cgctgccgcg
     1741 gccgccgccg ccggcaagct cgccggcctg ccgcccgg
//
LOCUS       DASVOR010000710         2021 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211427,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000710 DASVOR010000000
VERSION     DASVOR010000710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2021
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211427"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VF516_14000"
     CDS             <1..247
                     /locus_tag="VF516_14000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108839.1"
                     /translation="TCPADAALWTAPGWRELAFSIDGEHRFSYQYTPESSGLAATLRA
                     VGDVACDGTVRVVELQLTVAGGQVRRAWSHRTTAPPP"
     gene            312..728
                     /locus_tag="VF516_14005"
     CDS             312..728
                     /locus_tag="VF516_14005"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8108840.1"
                     /translation="MIAIPVALAILAGACGKNSQPPPPSSGTPIAEKESGPSRPAGQP
                     SQAQAMFNTVCVMCHGPDGTGNGPAASTLNPRPRNYTDPAWQASVTDDQIKEIILKGG
                     AGVGKSPSMPGQPQLQDHPDVLDGLVKIIRGFGKHP"
     gene            725..>2021
                     /locus_tag="VF516_14010"
     CDS             725..>2021
                     /locus_tag="VF516_14010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108841.1"
                     /translation="MIATSPDGQWAAVKRGREVMLLAGGAGPAAAGPATADRTTVRID
                     LPTDDADLVMVGSPSVLAVVTRGPAAGGEPCHRIALYQPPHPDAVAGHDLDGAMRIAG
                     LTGSRLVLVSRDGKAVTIVRIAGRALATQAIDPGSPVEFAVGLERNQVLFSLLRKLET
                     WDAVSGRPLLRMQLPLPPPPRTVGPAHGFLWLTRPGGDDILVYRMSDGRPFRHVLGVP
                     IDDVIYHPASPLLILVTARGLVRLHCYAHSLTRIDAPWQPGMELGQLVAGEDISLLGM
                     AEHDAEPWRVPIGGTGAPAITIDAPEAAGEPVITAADKLRAMRERSLQETLDRADRAD
                     SPAPPERAERPERPRLQRAGGLRISLADRTDPVSYLDRPNRSDLFHPLSRDDRPDRSD
                     LSSRDDRPERNERAERGDGIDPPDRGDRAAAVRSLALSAT"
BASE COUNT          284 a          795 c          702 g          240 t
ORIGIN      
        1 gacctgcccg gccgatgccg cgctgtggac cgcgccgggc tggcgcgagc tggcgttctc
       61 gatcgacggc gagcaccgct tcagctatca gtacactccc gagtcgagcg ggctggccgc
      121 cacgctgcgc gcggtcggcg acgtggcctg tgacggcacc gtccgcgtgg tcgagctcca
      181 gctcaccgtc gcgggcggcc aggtgcggcg cgcgtggtcg caccggacca ccgcgccgcc
      241 gccatagccc gggcggcggg tcgcaatcgc cgagcggtcg cggcacaata ggcgcgctta
      301 tgcgcagcct cgtgatcgcg atccccgtcg ccctcgccat cctcgccgga gcatgtggca
      361 agaacagtca gccgcccccg ccgagctccg gcacgcccat cgccgagaag gagagcgggc
      421 ccagccgccc ggccggccag ccgtcgcagg cccaggcgat gttcaacacc gtgtgcgtga
      481 tgtgccacgg ccccgacggc accggcaacg gtcccgccgc ctcgaccctc aaccccaggc
      541 cgcgcaacta caccgacccg gcgtggcagg ccagcgtcac cgacgaccag atcaaggaga
      601 tcatcctcaa gggcggcgcc ggcgtgggca agagcccatc gatgcccggc cagccccagc
      661 tccaggacca ccccgacgtg ctcgacgggc tggtcaagat catccgcggc ttcggcaagc
      721 atccatgatc gcaacgtctc ccgacggcca gtgggcagcg gtcaagcgcg gtcgggaggt
      781 gatgctactg gccggcggcg ccggcccggc ggcggccggc ccggcgacgg ccgaccgaac
      841 gacggttcga atcgacctgc cgaccgatga cgccgatctc gtgatggtcg ggtcgccgag
      901 cgtgctggcg gtcgtgaccc ggggcccggc tgcgggcggc gagccgtgcc accgcatcgc
      961 cctgtaccag ccgccacacc ccgacgcggt cgccggccac gacctcgacg gcgcgatgcg
     1021 gatcgccggg ctcaccggct cgcggctggt gctggtctcg cgcgacggca aggcggtgac
     1081 gatcgtgcgg atcgccggcc gcgcgctcgc gacccaggcg atcgaccccg gcagcccggt
     1141 cgagttcgcg gtcggcctcg agcgcaacca ggtgctgttc agcctgctcc gcaagctcga
     1201 gacctgggac gcggtgtccg gccgcccgct gttgcgcatg cagctgccgc tgccgccgcc
     1261 gccgcgcacg gtcggcccgg cgcacggctt tctgtggctg acccggcccg gcggcgacga
     1321 catcctggtg taccggatgt ccgacggccg gccgttccgc cacgtgctcg gcgtgccgat
     1381 cgacgacgtg atctatcacc ccgcatcgcc gctcctgatc ctggtcaccg cgcgcggcct
     1441 ggtccggctg cactgctacg cgcactcgct gacgaggatc gacgcgccct ggcagccggg
     1501 catggagctc ggccagctcg tggccggcga ggacatcagc ctgctcggca tggccgagca
     1561 cgacgccgag ccgtggcgcg tcccgatcgg cggcaccggc gcgcccgcga tcacgatcga
     1621 cgctccggaa gctgccggcg agccggtgat caccgcggcc gacaagctgc gcgcgatgcg
     1681 cgagcgcagc ttgcaggaga cactcgaccg cgccgaccgg gccgacagcc ccgccccacc
     1741 cgagcgcgcc gagcgccccg agcgtccccg cctccagcgc gccggcggtc tccggatcag
     1801 cctggcggac cgcaccgacc cggtcagcta cctcgaccgc ccgaaccgca gcgacctgtt
     1861 ccacccactc agccgtgacg accgcccgga ccgcagcgat ctgagcagcc gcgacgaccg
     1921 cccggaacgc aacgagcgcg ccgagcgcgg cgacggcatc gacccgcccg accgtggcga
     1981 tcgagctgcg gcggtgcggt cgctcgcctt gtcagcgacg g
//
LOCUS       DASVOR010000711         2978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_123614,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000711 DASVOR010000000
VERSION     DASVOR010000711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123614"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="VF516_14015"
     CDS             complement(<1..844)
                     /locus_tag="VF516_14015"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX8108842.1"
                     /translation="MYTHAKTVFAQALAEIDAAGLWKHERIISSPQAAHIRSEGREVV
                     NFCANNYLGLSSHPQVIAAARAALDDRGYGLSSVRFICGTQDRHKELEAAISAFLGTE
                     DTILYGSCFDANGGLFETLLGEDDAVISDSLNHASIIDGIRLCKAERLRYEHDDLDDL
                     EAKLKASAGKRLRLIATDGVFSMDGDIARVDAICDLAERYGALVMVDDSHATGFVGRT
                     GRGSPELRGCMDRIDVFTSTLGKALGGASGGFTSGRREIIDLLRNRSRPYLFSNTLAP
                     PIVAG"
     gene            complement(905..1336)
                     /locus_tag="VF516_14020"
     CDS             complement(905..1336)
                     /locus_tag="VF516_14020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108843.1"
                     /translation="MSSTPPPASPAAPILTTSADSIQYAVGNPSFRGRTVVKVTGSGA
                     VDVSFERGGKTDHYTGQLDADELRGLRDTLASHDPRALHSARATGKPDEARIELTIRG
                     TAGDSQVTLWDGEQWQMPSLRALVVAFNAIASKTSGGKVKY"
     gene            complement(1653..2627)
                     /locus_tag="VF516_14025"
     CDS             complement(1653..2627)
                     /locus_tag="VF516_14025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8108844.1"
                     /translation="MKILLTGAGGQIGHDLIGALVAGGHEVVSTDLAPRPPSHAHAGG
                     ESWQRLDVTDAAATVALMMEIKPDLVFHLAAILSARGEQDPRLAYDVNQTGTWNVLEA
                     CRRAKVKRLVFTSSIAVFGPLPGDGLPDPTPDDVALHPTTMYGVTKVSGELLCEYYRD
                     KFGVDCRGVRFPGLISAAMPGGGSSDYALFMYVDGVRKNGYEAFARADTRIPLMYMPD
                     GVRALLEVAMADRVRLTRAIYNIAAFSPRADEIAASVQTVLPGARFTFRPDPARQAIL
                     DSWPKSLDDSAARRDWGWKPRFDLDAMTRDLIPKIRRMLELGAILSSH"
BASE COUNT          403 a          995 c         1148 g          432 t
ORIGIN      
        1 agcccgccac gatcggcggc gcgagggtgt tcgagaacag gtacggccgc gaccggttgc
       61 gcagcagatc gatgatctcg cgccggcccg acgtgaagcc gcccgacgcg ccgccgagcg
      121 ccttgcccag cgtcgaggtg aacacgtcga tccggtccat gcagccgcgc agctcgggcg
      181 agccgcggcc ggtccggccg acgaacccgg tcgcgtggct gtcgtcgacc atgaccagcg
      241 cgccgtagcg ctcggcgaga tcacagatcg cgtcgacccg cgcgatgtcg ccgtccatcg
      301 agaacacgcc gtcggtggcg atcaggcgca gccgcttgcc ggcgctcgcc ttgagcttgg
      361 cctcgaggtc gtcgagatcg tcgtgctcgt agcgcagccg ctcggccttg cacagccgga
      421 tcccgtcgat gatcgaggcg tggttgaggc tgtcggagat caccgcgtcg tcctcgccga
      481 gcagggtctc gaacagcccg ccgttggcgt cgaagcacga gccgtacagg atggtgtcct
      541 cggtgccgag gaacgccgag atcgcggcct cgagctcctt gtggcggtcc tgggtgccgc
      601 agatgaaccg cacgctcgac aggccgtagc cgcgatcatc gagcgcggcg cgcgccgcgg
      661 cgatcacctg ggggtggctc gacagcccga ggtagttgtt ggcgcagaag ttgacgacct
      721 cgcggccctc gctgcggatg tgcgcggcct gcggcgacga gatgatgcgc tcgtgcttcc
      781 acagccccgc cgcgtcgatc tcggcgagcg cctgggcaaa cacggtcttc gcatgggtgt
      841 acatgggcgc tagcctagag cgccgcgagc cgttcgggta gggtagcgcc ggcagaccgg
      901 cgaatcagta cttgaccttg cccccggagg tcttgctggc gatcgcgttg aacgcgacca
      961 cgagcgcgcg cagcgatggc atctgccact gctcgccgtc ccacagcgtg acctggctgt
     1021 cgcccgcggt gccgcggatg gtgagctcga tgcgcgcctc gtcgggcttg ccggtcgccc
     1081 gggcgctgtg gagcgcgcgc ggatcatggc tggccagggt gtcgcgcagg ccgcgcagct
     1141 cgtcggcgtc gagctgcccg gtgtagtggt cggtcttgcc gccgcgctcg aagctgacgt
     1201 cgaccgcgcc gctgccggtg accttgacca cggtgcgccc gcgaaagctc gggttgccga
     1261 ccgcgtactg gatggagtcg gcggaggtgg tgaggatcgg cgccgcgggg ctggccggcg
     1321 gtggagtgga ggacatggcg ctgctcgagg gggtgggggc tggcgccgcg tgcgcgggcg
     1381 ctaccgtggg gacggggacc gtggtggcgg ccggcgtcgg gctggtgtgg ctgggaggtg
     1441 ccgcctgctc cccggaggaa gcgcatgctg cgaccgtggc gagggcgagc ccggcggtgc
     1501 ggaggatcat ggcagcttga gctcccagcc gggcaagatc cggtcctccc ctgcggaatc
     1561 ctgaccagcg tacaccatgt gtacgggcga gcccacggga tccttcctcc gtcgggccag
     1621 cgtccgggcg gaggcggccg ccggttgcgc gctcagtgcg acgacaggat cgcgccgagc
     1681 tcgagcatcc ggcggatctt ggggatcagg tcgcgggtca tcgcgtcgag gtcgaagcgc
     1741 ggcttccagc cccagtcgcg gcgcgcggcc gagtcgtcga gcgacttcgg ccaggagtcg
     1801 aggatcgcct ggcgcgccgg atcggggcgg aaggtgaacc tggcgcccgg cagcacggtc
     1861 tggaccgagg cggcgatctc gtcggcgcgc ggcgagaacg cggcgatgtt gtagatcgcg
     1921 cgggtcagcc ggacgcggtc ggccatcgcg acctcgagca gcgcgcggac gccgtcgggc
     1981 atgtacatca gcgggatccg ggtgtcggcg cgcgcgaacg cctcgtagcc gttcttgcgc
     2041 acgccgtcga cgtacatgaa cagcgcgtag tccgacgagc cgccgcccgg catcgcggcg
     2101 ctgatcaggc cggggaaccg gacgccgcgg cagtcgacgc cgaacttgtc gcggtagtac
     2161 tcgcacaaga gctcgcccga gaccttggtg acgccgtaca tcgtcgtcgg gtgcagcgcg
     2221 acgtcgtcgg gcgtggggtc gggcagcccg tcgccgggca gcgggccgaa caccgcgatg
     2281 ctggaggtga acaccaggcg cttgaccttg gcgcgccggc acgcctcgag cacgttccag
     2341 gtcccggtct ggttgacgtc gtaggccagg cgcgggtcct gctcgccgcg cgccgacagg
     2401 atcgccgcga ggtggaacac caggtccggc ttgatctcca tcatcagcgc gacggtggca
     2461 gcggcgtcgg tgacgtcgag ccgctgccac gactcgccgc ccgcgtgggc atggctcggc
     2521 gggcgcgggg cgagatcggt ggacacgacc tcgtggccgc cggcgaccag cgcgccgatc
     2581 aggtcatgtc cgatctgccc gcctgccccg gtgaggagga tcttcatgag gcgcgcatca
     2641 tccactggtc gtcgtcgggc ggcaaggccg ggatcgccct ccggtggcga cgcgtgtggc
     2701 cctccagcat ccggcggcgg cgacgatcag gtcggcgagc tcctccgggg cgagctggtc
     2761 ggcgctggcg gacgaggcgc agatcccggc gtgctacgat cacagcggcc atgaccgcgc
     2821 acgcccggct cgcagcgctc gccgcgaaga tcgaagcgga tcccggggcg ctggccgggg
     2881 tgcgcgacaa gggcgacgcg ctcgccgatc ggccggagct gtccgacggc gacgacgcgc
     2941 tgcgctggcg gatcgccgtg gtccgcgcgg tgatggcg
//
LOCUS       DASVOR010000712         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_240479,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000712 DASVOR010000000
VERSION     DASVOR010000712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240479"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(133..525)
                     /locus_tag="VF516_14030"
     CDS             complement(133..525)
                     /locus_tag="VF516_14030"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8108845.1"
                     /translation="MSQDFTAIMPFMRLCGIAITSATAGEVKGTLPWREDLCTTAGVV
                     HGGALMTFADSLGATCAFLNLPAGATTSTIESKTNFFRPVTKGAAHGTTTPLHVGRTT
                     IVVQTDIRDDAGKRVALVTQTQAVITRS"
     gene            complement(561..>2148)
                     /locus_tag="VF516_14035"
     CDS             complement(561..>2148)
                     /locus_tag="VF516_14035"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8108846.1"
                     /translation="RRVQALRTLARDLARTRQVLHDAIADDAGMPVPLRNTLLDAEGE
                     LAGQAALLSQTTAALLDEVEALRRTIGELQRGLTRIRLESARSLMLHAARTLRALQRS
                     TGVKVELRTFGEATEFDKAVAEHLTDPLTQLLRNAMAHGIETPGERVAHGKLALATIT
                     IRARQDGHSLVLEVSDDGRGIDTAAVSRRLVATGRWSAARARLADDADVLEALLATGV
                     STRGEADELAGRGVGIGMVRQSVAKLGGEIKVVSAAGKGTTFTLCMPLSASLAQAMLF
                     KVDGQVYAVSSVHVADTTHVEPHATTAMVRSDALPVVRLEQLLGHGAATDRRPALVVT
                     FAGKRVVCTVDKIVGAREIIIKPLGPLLAPLTVYAGATISGSGKVQLILDPAQLVRRA
                     YPDAAEAAIDPRSAPMMLAGRALVVDDSRAIREAMTSMLGSEGWIVDVAEDGVRALQL
                     ARQQRYDLVVTDLEMPELGGFDFIARLRSDDRLKATPVVVITSRASPEHRRRARDLGV
                     RALVAKPITRRKLLEALAAR"
BASE COUNT          266 a          839 c          752 g          291 t
ORIGIN      
        1 cacgtcgagc ccggcgcccg cccgcgccga gctccggccg gcgccggcgg caagctccgg
       61 ctcggcgtgt cgcaccgcgg cccgcgcgac gacgacggcg agcgcaagaa gcgcggcaag
      121 cgccgcacgt gatcacgatc gcgtgatcac cgcctgggtc tgggtcacca gcgcgacgcg
      181 cttgcccgcg tcgtcgcgga tgtcggtctg cacgacgatc gtcgtgcgcc cgacgtggag
      241 cggcgtcgtc gtgccgtgcg cggcgccctt ggtgaccggc cggaagaagt tggtcttgga
      301 ctcgatcgtg gaggtcgtcg cgccggccgg caggttgagg aacgcgcagg tcgcgccgag
      361 gctgtcggcg aacgtcatca gcgcgccgcc gtggaccacg ccggcggtgg tgcacagatc
      421 ctcgcgccag ggcagggtgc ccttgacctc gccggcggtc gccgacgtga tcgcaatccc
      481 gcacagacgc atgaacggca tgatcgcggt gaagtcctgg ctcatggcgg ctcctttgcg
      541 ccgcgagcat atgccgcacg tcaccgcgcc gcgagcgcct ccagcagctt gcgccgcgtg
      601 atcggcttgg cgaccagcgc ccgcacgccg aggtcgcggg cgcgccggcg gtgctcgggg
      661 ctggcgcgcg acgtgatcac cacgaccggc gtcgccttca gccggtcgtc gctgcgcagc
      721 cgcgcgatga agtcgaaccc gccgagctcg ggcatctcga gatcggtgac caccaggtcg
      781 tagcgctgct ggcgcgccag ctgcagcgcc cgcacgccgt cctcggcgac gtcgacgatc
      841 cagccctcgc tgcccagcat gctcgtcatc gcctcgcgga tcgcccgcga gtcgtcgacc
      901 accagcgcgc ggccggccag catcatcggc gccgatcgcg gatcgatcgc cgcctccgcc
      961 gcgtcggggt acgcccgccg caccagctgc gccggatcga ggatcagctg gaccttgccc
     1021 gagccgctga tcgtcgcccc ggcgtacacc gtgagcgggg cgagcagcgg cccgagcggc
     1081 ttgatgatga tctcgcgcgc gccgacgatc ttgtcgaccg tgcacaccac gcgcttgccg
     1141 gcgaacgtca ccaccagcgc cggccgccgg tcggtcgccg cgccgtggcc gagcagctgc
     1201 tccagccgca ccaccggcag cgcgtcgctg cgcaccatcg ccgtcgtcgc gtgcggctcg
     1261 acgtgcgtgg tgtccgcgac gtggacgctc gacaccgcgt agacctggcc gtcgaccttg
     1321 aacagcatcg cctgggcgag cgacgccgac agcggcatgc acagcgtgaa cgtcgtgccc
     1381 ttgcccgccg ccgataccac cttgatctcg ccaccgagct tggcgaccga ctgccgcacc
     1441 atcccgatgc cgacgccgcg cccggccagc tcgtcggcct cgccgcgcgt cgagaccccg
     1501 gtggcgagca gcgcctcgag cacgtcggcg tcgtcggcca gccgcgcccg cgccgcgctc
     1561 caccgcccgg tcgcgaccag ccgccggctg accgccgcgg tgtcgatccc gcgcccgtcg
     1621 tcgctgacct ccagcaccag cgagtggccg tcctggcggg cccggatcgt gatcgtggcg
     1681 agcgccagct tgccgtgcgc cacgcgctcc cccggcgtct cgatcccgtg cgccatcgcg
     1741 ttgcgtagca gctgggtcag cggatcggtc aggtgctcgg ccaccgcctt gtcgaactcg
     1801 gtcgcctcgc cgaacgtgcg cagctcgacc ttgaccccgg tcgaccgctg cagcgcgcgc
     1861 agcgtgcgcg ccgcgtgcag catcaggctg cgcgccgact ccagtcggat ccgggtcagc
     1921 ccgcgctgca gctcgccgat cgtccggcgc agcgcctcga cctcgtcgag cagcgccgcg
     1981 gtggtctggc tgagcagcgc ggcctgcccg gcgagctcgc cctcggcgtc gagcagcgtg
     2041 ttgcgcagcg gcaccggcat cccggcgtcg tcggcgatcg cgtcgtgcag cacctgccgg
     2101 gtgcgcgcca ggtcgcgcgc cagcgtgcgc agcgcctgga cccgccgc
//
LOCUS       DASVOR010000713         5242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_269632,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000713 DASVOR010000000
VERSION     DASVOR010000713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5242
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269632"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VF516_14040"
     CDS             complement(<1..758)
                     /locus_tag="VF516_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEX8108847.1"
                     /translation="MSDELAIAKRKADHLEVAASGRAEFVKSTLLEHVHLFHQALPEL
                     TLDEIDLSTVLVGKRLAAPLVITGMTGGTAEAASVNRDLARAAQAAGVALGLGSQRAM
                     AEHPELAGTYQIRDVAPDVVLLGNIGGVQAIAMGPDRVVALATQIGADAICVHLNPGQ
                     ELIQDRGDRDFRGVRDAIARLVEVSPVPVIVKETGCGLSVEAARALVAAGVHTVDVSG
                     AGGTSWVAVEAVRAAEGSDAAALGAELWDWGIPTA"
     gene            1169..1666
                     /locus_tag="VF516_14045"
     CDS             1169..1666
                     /locus_tag="VF516_14045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108848.1"
                     /translation="MNAGQFIQWQDLFAIVDDGSIPISRYGVIETAMREQAKTYPQGI
                     AVLVILPPDARPPPEEVRTTVKNVLMRLVSSLSCLTYVVEGTGFKGVAARATLVGMKI
                     FASRPYPIYVETSVREALAKITSHMTNGHAVSTEVILKAISEARLVWQAPSQARNVDH
                     ELTLK"
     gene            complement(1696..2319)
                     /locus_tag="VF516_14050"
     CDS             complement(1696..2319)
                     /locus_tag="VF516_14050"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8108849.1"
                     /translation="MIHSVPWHDKGIYGAFLAQTYYYVNHSTRLLALAASRFQSSEER
                     IHRRFLKHATEEMSHEILALRDLKHLGLSLESYHEFPSTRAFYQTQYYMIEHVSPWSF
                     WGYILMLEGLALTEGTWLFREIKQHHGEKAGSFVKVHAIEDIGHMAEAEQALRLLPDS
                     QRPLVLEQIANSCFYYSAMLEQCKGYAAVHRKGGVGSGDNATAAAIA"
     gene            complement(2380..3087)
                     /locus_tag="VF516_14055"
     CDS             complement(2380..3087)
                     /locus_tag="VF516_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108850.1"
                     /translation="MLAADGATPTMTSLSLKIFYPDAISTGEIHDYNEAFAFWYEIWL
                     ETRREVDATLAAPSDSFSRQSEILVLYSDGKPIATCCHRYVDLRHHCVLYDSYFTSAI
                     WPASVKSLIPGLGHTCVLGSHIFIHPAFRKCRGGLPIKNIVCALSMTHVNATRPDVLL
                     GAARIDKGIHKVFHDAGAVSLRADASWYHIPVDLIALFPNKSPMQIDAQYDDIVRSIG
                     KTCHRFAVNYLQRTTAT"
     gene            complement(3838..>5242)
                     /locus_tag="VF516_14060"
     CDS             complement(3838..>5242)
                     /locus_tag="VF516_14060"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEX8108851.1"
                     /translation="TVYGLTSLDPENWDTRRFLFAGIVPGMVLVGMLSVVAVTVAVMK
                     KLPRQKFDLGELGRSLALAFPELCIPFGVIVGLAVGFDLPQVAALIVVYTIVLEVGVA
                     RLLVRLGIGWGQPLAPRVLWSVSREAMAMVGAIFIIIFASTALTNFMVTDEVPRKLVA
                     WTQSHVESKIVFLLAINVILLIVGTVMDIFSAIVVVLPLIGPIAKAYGIDPYHLGVIF
                     LLNLEVGYLHPPVGLNLFITSVKFQRPITEVMWATIPFLVTMIIALLTITYVPKLTVV
                     PEAERTGRTADLVAMVHTEVERLQVGGDVTLVDAAGNPLRDAHGQPIVRHIADCDKLD
                     GIAKDGCQNLFFSVKDCRGKPDQKTCEHKAIASWVVKSLNGSDDGTSPIIVVTEVPLV
                     DSNGDPVNDKAGNRRVCKLPACATVIDKDSCRDLFITASNCKILPPDGGDIDACVKDR
                     TAAWYDQNNGSLDSSCP"
BASE COUNT          906 a         1685 c         1753 g          898 t
ORIGIN      
        1 gcggtcggga tgccccagtc ccacagctcg gcgccgagcg ccgctgcgtc cgagccctcg
       61 gcggcgcgca ccgcctcgac cgcgacccag ctcgtgccgc cggcgccgga cacgtcgacg
      121 gtgtggaccc cggccgcgac cagcgcgcgc gcggcctcga ccgacaggcc gcagccggtc
      181 tccttgacga tcaccggcac cggcgagacc tcgaccagcc gcgcgatcgc gtcgcgcacg
      241 ccgcggaagt cgcggtcgcc gcggtcctgg atcagctcct ggccggggtt gaggtggacg
      301 cagatcgcgt cggccccgat ctgcgtggcg agcgcgacca cccggtccgg gcccatcgcg
      361 atcgcctgca cgccgccgat gttgccgagc aggaccacgt cgggcgcgac atcgcggatc
      421 tggtaggtcc cggcgagctc ggggtgctcg gccatcgcgc gctgcgagcc gaggccgagc
      481 gcgaccccgg cggcctgggc cgcgcgcgcc agatcgcgat tgaccgaagc cgcctcggcg
      541 gtgccgccgg tcatcccggt gatcaccagc ggcgccgcca gccgcttgcc gaccagcacc
      601 gtcgagaggt cgatctcatc gagcgtgagc tcgggcagcg cctggtggaa caggtggacg
      661 tgctccagca aggtcgactt gacgaactcg gctcggcccg aggcggcgac ctcgaggtgg
      721 tcggccttgc gcttggcgat cgccagctcg tctgacacgc gctgtcttta ccatgccggc
      781 cacaccgcgc cgaagcaccc ccgggcgcgc cggcgcacgg atcgcggccg ccgggccggg
      841 cggcgagcga caccgaatcc gcctcgccgc ggccgctgtt ccgcgtgcgg agcgagcgat
      901 cgcggcgaaa tttccagcca cagtgtgggg cgccctcacg tccacgatcg tccgcgtgat
      961 gcgcaggtga gatgatggcg ctgagggcgg tttcgctcga ttgtacagtc gacatgaaga
     1021 tcagcgccgg ggcgccgtgc cgctgacgca tcggatcgga cgcacgaggc cgctgtgatg
     1081 tgagtctccc cgagcgaaaa aaccacagcg cagggttgtc ggcgttcgtg ccagagtcat
     1141 atatgaatca ggtcacctgg aggtcgccgt gaacgcaggg cagttcatcc aatggcagga
     1201 tctgttcgcg atcgtcgacg acggctcgat cccgatctcg cggtacggag tgatcgagac
     1261 cgccatgcga gagcaggcga aaacttatcc tcagggcatc gcggtcctcg tgatcctacc
     1321 gcccgacgcc aggccgccgc cggaggaggt ccggacgacg gtcaagaacg tcctgatgcg
     1381 cctggtgagc tcgctgtcgt gcctgaccta cgtggtcgag gggaccgggt tcaagggggt
     1441 cgcggcgcgc gccacgctgg tcgggatgaa gatcttcgcg tcgcgcccct acccgatcta
     1501 cgtcgagact tcggtgcgcg aggcgctcgc caagatcacg tcgcacatga ccaacggcca
     1561 cgcggtctcg accgaggtca tcctcaaggc gatctcggag gcccgcctgg tctggcaggc
     1621 gccgtcgcag gcgcggaacg tcgaccacga gctcacgctc aagtgaaccg cgggccgcgc
     1681 ggcgcgcggg ccccgtcagg cgatcgcggc cgcggtcgcg ttgtcaccgg agccgacccc
     1741 gcccttgcgg tggaccgccg cgtacccctt gcactgctcc agcatggcgc tgtagtagaa
     1801 gcacgagttg gcgatctgtt cgagcaccag cgggcgctgg ctgtcgggca gaagtctcag
     1861 cgcctgctcg gcctcggcca tgtggccgat gtcctcgatc gcatggacct tgacgaagct
     1921 gccggccttc tcgccgtggt gctgcttgat ctcgcggaac agccaggtgc cctcggtcag
     1981 cgcgaggccc tcgagcatga ggatgtagcc ccagaagctc cacgggctga cgtgctcgat
     2041 catgtagtac tgggtctggt agaacgcgcg cgtcgacgga aactcgtggt agctctccag
     2101 cgacaacccg aggtgcttga ggtcgcgcaa cgcgaggatc tcatggctca tctcctcggt
     2161 cgcgtgcttg aggaagcgac ggtggatgcg ctcctccgac gactggaacc ggctcgcggc
     2221 gagcgccagc agccgcgtcg agtgattgac gtagtagtag gtctgcgcca ggaatgcccc
     2281 gtagatcccc ttgtcgtgcc acgggacact gtgaatcacc ctgcccaact cgttcagatg
     2341 aacctggagc tcgcgaaaca tcttggcctc cattcgcatt caggtcgccg tcgtgcgttg
     2401 caggtaattg accgcaaagc ggtgacaggt cttgccgatc gaccgcacga tgtcgtcgta
     2461 ctgcgcgtcg atctgcatcg gagacttgtt cggaaacagc gcgatcaggt cgaccgggat
     2521 gtggtaccag ctggcgtcgg cgcgcagcga gaccgcgccc gcgtcgtgaa acaccttgtg
     2581 gatccccttg tcgatccgcg cggcgccgag caggacgtcg ggccgggtgg cgttgacgtg
     2641 agtcatcgac agcgcgcaca cgatgttctt gatcggtagg ccccctcgac acttgcggaa
     2701 cgcggggtgg atgaagatgt ggctgccgag cacgcaggtg tggccgagcc ccgggatcaa
     2761 cgacttgacg ctggccggcc agatcgcgct cgtgaagtac gagtcgtaga gcacgcagtg
     2821 atggcggagg tcgacgtagc ggtggcagca cgtcgcgatc ggcttgccgt cgctgtacag
     2881 caccaggatc tcgctctgtc gcgagaagct gtcggagggt gcggcgagcg tcgcatcgac
     2941 ttcccgccgg gtctcgagcc agatctcgta ccagaacgcg aacgcttcgt tgtagtcgtg
     3001 gatctcgccc gtcgagatcg cgtccggata gaagatcttg aggctcaagg acgtcatcgt
     3061 cggcgtggcg ccgtcggcag cgagcatgtg gttcaccctc gctccgcacg ctgccgggcc
     3121 gtcatccgga gcgcatcacg cgcgaccagg gccgagtggc ctgtcccggt caaccggtgt
     3181 atgtgtgact cacgaaccac ggagtaggtc gcagctagga gggaaatctg cgccagacct
     3241 cgaccagctg ttccataaat ctgcaccccg ggtgggggcg ccagccccgc aggatctaag
     3301 tgcgatctgc attgcgtttt tatcgcatct atctcaagcc taatgaaagt aggctggatg
     3361 gaattcaatg ctgctcgatg gaattttcct tatgtaatca attgtatgta tattatacat
     3421 caaggctgaa attacttaac tatcattgaa aatagaaggg accctcgatt ggaacgctct
     3481 gtcgtggata ctgtacggat attcaggtat agatgtccgt tccgcgacgc gccaggtgtt
     3541 cgagctcaca taattatgtg agcgaatata caaaataatc ctggtcagcc gcgctttttg
     3601 taagcatcga tgctgatcaa tgcagccgcg tgctgcagga acaagggcag ccacgcgcgc
     3661 agacgcacac cgatcgtcag ccattatccg acaacgatcg tgtcttgttg actgcagtaa
     3721 attgctacat acgatgcagc aatttactac tgctctgaca cttgtgtact gccccgcttg
     3781 gcgctgggcg gagcacgagt taccgcgctc ggcggtccag cgccggccgt gatcgaatca
     3841 cgggcagctg ctgtcgagcg agccgttgtt ctgatcgtac caggcggcgg tgcggtcctt
     3901 gacgcaggcg tcgatgtcgc cgccgtcggg cggcaggatc ttgcagttcg acgcggtgat
     3961 gaacaggtcg cggcacgagt ccttgtcgat caccgtggcg cacgcgggga gcttgcacac
     4021 ccgccggttg ccggccttgt cgttgaccgg atcgccgttg ctgtcgacca gcggcacctc
     4081 ggtgaccacg atgatcgggc tggttccgtc gtcggagccg ttgaggctct tgaccaccca
     4141 cgacgcgatc gccttgtgct cgcaggtctt ctggtcgggc ttgccgcggc agtccttgac
     4201 cgagaagaac aggttctggc agccgtcctt ggcgatcccg tcgagcttgt cgcagtcggc
     4261 gatgtgccgg acgatcggct ggccgtgcgc gtcgcgcagc ggattgcccg cggcgtcgac
     4321 cagcgtcacg tcgccgccga cctgcaggcg ctcgacctcg gtgtggacca tcgcgaccag
     4381 gtccgcggtc cggccggtcc gctcggcctc gggcacgacg gtcaacttcg gcacgtaggt
     4441 gatcgtcagc agcgcgatga tcatcgtgac caggaacggg atcgtcgccc acatcacctc
     4501 ggtgatcggg cgctggaact tgacgctggt gatgaacagg ttgaggccga ccggcgggtg
     4561 gaggtacccg acctcgaggt tgagcaggaa gatcacgccg aggtggtacg gatcgatccc
     4621 gtaggccttg gcgatcgggc cgatcagcgg cagcacgacg acgatcgccg agaagatgtc
     4681 catcaccgtc ccgacgatca gcaggatcac gttgatcgcg agcaggaaca cgatcttcga
     4741 ctcgacgtgg gactgggtcc acgcgaccag cttgcgcggg acctcgtcgg tgaccatgaa
     4801 gttggtcagc gcggtcgacg cgaagatgat gatgaagatc gcgccgacca tcgccatcgc
     4861 ctcgcggctg acgctccaca gcacgcgcgg cgcgagcggc tggccccagc cgatcccgag
     4921 ccggacgagc agccgcgcca cgccgacctc gagcacgatg gtgtagacca cgatcagcgc
     4981 ggcgacctgc ggcaggtcga agccgaccgc gaggccgacg atcacgccga acgggatgca
     5041 cagctcgggg aacgcgagcg ccaggctgcg gccgagctcg ccgagatcga acttctggcg
     5101 cggcagcttc ttcatcaccg cgacggtgac cgcgaccacc gacagcatgc cgaccagcac
     5161 catgccgggc acgatgccgg cgaacaggaa ccgccgggtg tcccagttct cgggatcgag
     5221 gctggtcagc ccgtagaccg tg
//
LOCUS       DASVOR010000714         3796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_240502,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000714 DASVOR010000000
VERSION     DASVOR010000714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240502"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF516_14065"
     CDS             <1..291
                     /locus_tag="VF516_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108852.1"
                     /translation="VMMAGVIVAGVGVISHSLAWRNVDVVKRDRDPQRYLDDRDKLII
                     ERDIAYGAYAVGGVVLATGLVLALTARSTGEGAQVSAAVTPGGATLSVAWSR"
     gene            279..1616
                     /locus_tag="VF516_14070"
     CDS             279..1616
                     /locus_tag="VF516_14070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108853.1"
                     /translation="MVAMRLRLEAAVAAASAFGAACLTIQPYQGPGGPALVDYSVDGR
                     GGATVTTPSLALHFAGGSGFHFPDQLLIDNVDVMGHAASPCWSQSGTGISVVPMPRVS
                     GDQVDAALPTMTSQLQAKLSGPAVVQVEVDWTTRWTFPAGEMCSTNLNHIPGGSSTFT
                     VFPDGQIVRNDHLTDVNPGMEQIMPGQCVCPDTPMIGGELINNQFILNAFWTFDRTRF
                     PEQIGLGNNPTPDPLGIAPDKIVTNYSTICLNSMGGKYQIASAWVLPPKTPDVSPLAA
                     AYGYDTLLSLGVEKPGTPNLQFPWDVHGALILEQSSCTAAFNRAIAYTTPQKLAITSG
                     SATADLVRSQLDGMYGGDPGNGNAPGFDVSDGPTTLSLAAPRPDEPPPPPLTGSFTVW
                     LRFPRGSVPGVPIPTRKGATDGWFVPQRVDDQNWIVWFRDPLQAGETITIQPN"
     gene            1828..2511
                     /locus_tag="VF516_14075"
     CDS             1828..2511
                     /locus_tag="VF516_14075"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEX8108854.1"
                     /translation="MSWFGNYLRSSIGAKHIMAVTGLLLLLFAIVHMIGHLQMFGGQD
                     VYNAYAHFLQELWEVKWPVRAGLLALLVIHVAVAIGLVARNRAARPAGYVRYRPVTSS
                     WVGRVMAGTGLIVFAFLVFHILHFTLGQVQPAYFHVMDPKDRWDAYSMFVRGFQSPAI
                     YVAYLIGIAVLALHLGHGASSWLQSLGLRHPKYPADRLGPALAAILFVGYMVPPTAVV
                     LGILRLPGA"
     gene            2595..3796
                     /gene="sdhA"
                     /locus_tag="VF516_14080"
                     /pseudo
     CDS             2595..3796
                     /gene="sdhA"
                     /locus_tag="VF516_14080"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase (quinone) flavoprotein
                     subunit"
BASE COUNT          589 a         1388 c         1231 g          588 t
ORIGIN      
        1 gtgatgatgg ccggcgtgat cgtcgccggc gtaggcgtga tcagccactc cttggcgtgg
       61 cggaacgtcg acgtcgtgaa gcgcgatcgc gatccgcagc gctacctcga cgatcgcgac
      121 aagctcatca tcgagcgcga catcgcgtac ggcgcctatg cggtcggcgg cgtcgtgctc
      181 gccaccgggc tcgtgctcgc gctgaccgcg cgcagcactg gcgagggcgc gcaggtctcg
      241 gcggcggtca ctccgggagg cgcgacgctg tccgtcgcgt ggtcgcgatg aggctccggc
      301 tcgaagcggc ggttgcggcg gcgtccgcgt tcggcgccgc ctgcctcacg atccagccgt
      361 accagggccc tggtggtccg gccctggtcg actattccgt cgacggccgc gggggcgcca
      421 ccgtcacgac ccccagcctc gcgctgcact tcgccggcgg cagcggcttc cacttccctg
      481 accagctgct gatcgacaac gtcgatgtca tgggacacgc ggcctccccg tgctggagcc
      541 agagcgggac gggcatctcg gtggtgccga tgccgcgggt cagcggagat caggtggacg
      601 ctgcgctccc gaccatgacc agccagctcc aggcgaagct gagcggcccc gcggtggtgc
      661 aggtcgaggt cgactggacg acccgctgga ccttcccggc cggggagatg tgcagcacga
      721 acttgaatca catcccgggc ggctcatcga cgttcaccgt gttcccggac ggccagatcg
      781 tgcgtaacga tcacctgacc gacgtgaacc ccgggatgga gcagatcatg ccgggccagt
      841 gcgtgtgccc cgacacgccc atgatcgggg gcgagctcat caacaaccag ttcatcctga
      901 acgcgttctg gacgttcgat cgcacgcggt tcccggagca gatcgggctg ggcaacaacc
      961 cgaccccgga tccactcggg atcgcgcctg acaagatcgt cacgaactac agcacgatct
     1021 gcctcaactc catgggtggc aagtaccaga tcgcctcggc gtgggtgctt cccccgaaga
     1081 ccccggatgt gtcgccgctc gcagcggcat acggctacga cacgctcctg tcgctcggtg
     1141 tcgagaagcc gggcaccccg aacctccagt tcccgtggga cgtccatggc gcgctgatcc
     1201 tcgagcagtc cagctgcacc gccgcgttca accgcgcgat cgcctacacc accccgcaga
     1261 agctcgcgat caccagcggc tccgccacgg ccgacctggt gcggtcgcag ctcgacggta
     1321 tgtacggcgg cgatccgggg aacggcaacg cccccggttt cgacgtctcc gacggcccca
     1381 ccacgctgag cttggcggcg ccccggcccg acgaaccgcc gcctcctccg ctcacgggct
     1441 cgttcacggt gtggttgcgg ttcccccggg ggtcggtgcc gggcgtgccg atcccgaccc
     1501 gcaagggggc gaccgacggg tggttcgtgc cccagcgggt cgacgatcag aactggatcg
     1561 tgtggttccg ggacccgctg caggccggcg agacgatcac gatccagccg aactgaccgg
     1621 cgcagccgcc ggcggacgcg gcccgcggct gcgaacgcca ccgagccggc gcaaatctcg
     1681 cgcggcgttc accgaccgcg accgtcgatc gcggcgcgcc cgccttgccc gccatcgccg
     1741 gtcgtggtgg atgatcgaac ccgtgcgacc gggagccgca tcgaactgcc gcaggccgcg
     1801 caccgcggaa cgccagagga cctcgcgatg agctggttcg ggaactacct tcgctcctcg
     1861 atcggagcca agcacatcat ggccgtcacc ggcctgttgc tgctgctgtt cgcgatcgtc
     1921 cacatgatcg gccacctgca gatgttcggc ggccaggacg tgtacaacgc ctacgcacac
     1981 ttcctgcagg agctgtggga ggtcaagtgg ccggtgcggg cggggttgct cgcgctgctg
     2041 gtgatccacg tcgcggtcgc gatcggcctg gtcgcgcgca accgcgccgc gcggcccgcc
     2101 ggctacgtcc gctaccggcc ggtgacctcg tcgtgggtcg gccgggtgat ggcgggcacc
     2161 gggctgatcg tgttcgcgtt cctggtgttc cacatcctgc acttcacgct cggccaggtc
     2221 cagcccgcct acttccacgt gatggatccc aaggaccgct gggacgcgta ctcgatgttc
     2281 gtacgcggct tccagagccc ggcgatctac gtcgcatacc tgatcgggat cgcggtgctc
     2341 gcgctgcacc tcggccacgg cgcgtcgagc tggctgcagt cgctcggcct gcgccacccc
     2401 aagtaccccg ccgaccggct cggcccggcg ctcgccgcga tcctgttcgt cggctacatg
     2461 gtgccgccga ccgcggtggt gctcggcatc ctccggctcc cgggggcctg aagaggcatg
     2521 ccatgacgct caagctcgac ccacgcaccc ccgccggacc gatcgagcag caatggtcga
     2581 acgcccggtt cgagatgaag ctggtcaatc cggccaacaa gcgcaagtac aaggtgatcg
     2641 tcgtcggctc ggggctcgcc ggcggcgcgg ccgccgccac gctcgccgag ctcggctacg
     2701 acgtcgcgtg cttctgcttc caggactcgc cgcgccgcgc gcactcgatc gccgcgcagg
     2761 gcgggatcaa cgcggccaag aactaccaga acgacggcga ctcggtccat cggctgttct
     2821 acgacaccgt caagggcgga gacttccggg cccgcgagtc caacgtccac cggctcgccg
     2881 aggtctcggt cgacatcatc gaccagtgcg ccgcgatggg tgtgccgttc gcgcgcgagt
     2941 acggcggcac gctcgccaac cgcagcttcg gcggcgccca ggtgtcgcgc acgttctacg
     3001 cccgcggcca gaccggccag cagctcctgc tcggcgtgta cgccgcgctg tcgcggatga
     3061 tcggagccgg caaggtgcgc atgttcccgc gcaccgagat gctcgacgtc gtcgtcgacc
     3121 ccggctcggg ccgcgcgtgc ggcatcgtcg tgcgcgacct cgtcaccggc gtcatcacct
     3181 cgcacgctgc ccacgcggtc gtcctcgcca ccggcggcta cggcaacgtg ttctatctct
     3241 ccaccaacgc caagggctgc aacgtcactg ccacctaccg cgcctacaag cgcggcgcag
     3301 gattcgccaa tccctgcttc acatagattc atccgacctg cattccggtc gccggtgatt
     3361 atcaatccaa actgaccctc atgtccgagt cgctccgcaa cgacggccgg gtctgggtcc
     3421 ccaggagcaa ggacgacgtc ggcaagcacc ccgatcagat cgccgagacc gcgcgcgact
     3481 attacctcga gcgcaagtat cccagcttcg gcaacctgtc gccgcgcgac atcgccagcc
     3541 gcgccgccaa ggagcaatgc gacgagggtc gcggcgtcgg cccgggtggg cgcggcgtct
     3601 acctcgactt ccgcgacgcc ctgcgccgcg acggcgagga caccatccgc gacaagtacg
     3661 gcaacctgtt cgagatgtac gagcgcatca cgggcgaaaa cccctacaag gtcccgatgc
     3721 gcatctaccc cgccgtccac tacaccatgg gcggcctctg ggtcgattac caccttcaaa
     3781 ccaccattgc cggcct
//
LOCUS       DASVOR010000715         2558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415419,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000715 DASVOR010000000
VERSION     DASVOR010000715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2558
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415419"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="VF516_14085"
     CDS             complement(<1..435)
                     /locus_tag="VF516_14085"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8108855.1"
                     /translation="MPPVACRMGTASDLELQLHDPSGTVSREHAELVPVEVDDERLWK
                     VHELRSKNGLYCDGEPLWAFVLRPGLEVRLGSLRLIAESMELIGLLSFVRRCLGWVAE
                     RQEHVDQALQSLRDWAAQRAELVIVGDGDLQPVVRRLHNLVIG"
     gene            complement(619..801)
                     /locus_tag="VF516_14090"
     CDS             complement(619..801)
                     /locus_tag="VF516_14090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108856.1"
                     /translation="MDCALDEPRESTEPLAFAQGGVLELDRGREDKQHHVAVDCALDE
                     PRESTEPLAFAQTPNQ"
     gene            complement(1022..>2558)
                     /locus_tag="VF516_14095"
     CDS             complement(1022..>2558)
                     /locus_tag="VF516_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8108857.1"
                     /translation="SFAPAGSHLAVGTYDGVASIWELPGSVHVRSLRDAGASVDAVAF
                     SRDGVLIAAASRDGTEQVWDAASGGLRAQFNSHHDKIYAVEFSISGDLILSAGADGAV
                     VVSNVTTGMPVARLEGPKGLIFAAHFDSESRRVVGASFDGTARVWDAAPPYRRWDSPQ
                     IGAECDTEESLVPDQRFIALSCRDHGTHVWDTARSELLAELPGVTPVEGNYSSALPAL
                     TATGDRAAIARGNVVEVYALPSGQLLRTIVHPAAVNAVAFAPAGHDLVSGAVDGSLLF
                     AHDESDPVALPTSSAGIDAAAILADGRVVVADAGGRLRVIGADRNALLMDVAASSRIR
                     LLRPSPDGRWLITISTASEQTSPVLWDLGQYRLVARLEGHVGRVFTARFVAVGGGREI
                     LTAGSDGTARLWDAATGRLRQSFRGDSHFLVDAAVAPGGSVLVAGGSDGFLRFWDTSN
                     GRLLWMLQAHRSYVIGVHYEGDDLITRGFAGDVARWALPSPDAIIESCHAGACASAAL
                     AGK"
BASE COUNT          475 a          904 c          773 g          406 t
ORIGIN      
        1 cccgatgacg aggttgtgca ggcgacgcac caccggctgc agatcaccat cgccaacgat
       61 gaccagctcg gcgcgctgcg ccgcccagtc acggaggctc tgcagcgcct gatcgacgtg
      121 ctcttgtcgc tcggccaccc agcccaggca ccgccgcacg aatgacagaa ggccgatcag
      181 ctccatgctc tccgcgatga ggcgcaacga gccgaggcgc acctcgagtc ccggccgcaa
      241 gacgaaggcc cacagaggtt cgccgtcgca atataggccg ttcttcgacc gcagttcgtg
      301 gaccttccag aggcgctcgt catcgacctc gaccggcacg agctcggcat gctcgcgcga
      361 gaccgtgccg ctcgggtcgt gtagctgcaa ctccaggtcg cttgccgttc ccatccggca
      421 cgcgaccggt ggcacgggca gaccgtacac gcggtccgtg ctgtgctcgc gaagccggat
      481 gacggcatcc cggatgcgcc agctacgccg aggaacggtc atcgttgaga cgtcaggcat
      541 caccagccct ccagccactt cctctttcgc atcccctgcc gcacgttgac aagctttgcc
      601 tgtgagctct gcgccacctc actgattcgg cgtctgtgcg aacgccaacg gttcggtgga
      661 ctcccgcggc tcgtcgagcg cacagtccac cgcaacgtgg tgctgcttat cctcgcgtcc
      721 ccgatcgagc tcgagcacgc cgccttgtgc gaacgccaac ggttcggtgg actcccgcgg
      781 ctcgtcgagc gcgcagtcca ccgcaacgtg gtgctactta cttagctggc tccgcagcag
      841 ccgcagcctg gagcggacaa cccgtgccgg cgtcttgcgt gcccacaact cggtcgtcgg
      901 ccccagcggc gaccaggacg agatccatct gagtgagagc ccggatcgtt tcagaacgga
      961 gggtgagctt gcgggttgat ttcttcatca tgctatgata gctcgcccgc gttctggccg
     1021 cttattttcc tgccagcgcc gccgatgcgc atgcgccggc gtggcacgac tcgatgatcg
     1081 cgtctggaga cggcagcgcc caacgtgcga catctccggc gaaacccctc gtgatgaggt
     1141 cgtcgccttc gtagtgcaca ccgatgacgt aggacctgtg cgcttgcagc atccagagca
     1201 agcgcccgtt cgacgtgtcc cagaaccgca ggaacccatc gctaccgccc gcaacgagca
     1261 ccgagccgcc aggagccacc gccgcatcta cgaggaaatg tgaatcccct cgaaagctct
     1321 ggcgtaggcg accggttgcc gcgtcccaga gccgcgccgt tccatccgac ccagccgtga
     1381 ggatctcgcg cccaccgccg acggccacga aacgcgctgt gaatacccgt cctacgtgac
     1441 cctcgagccg cgcgacgagc cgatactgac caagatccca cagtaccggc gaggtctgct
     1501 cgctcgcggt cgagatggtg atcagccacc tgccatccgg tgatggtcgc agcaacctga
     1561 tgcgggatga tgctgcgacg tccatcagca gcgcgttgcg gtccgcccca atgactcgca
     1621 accggccccc ggcatcagcc accaccaccc gaccatcagc gagaatggcc gcagcatcta
     1681 tcccggcgga tgatgtcggc aacgcgaccg gatcactctc gtcgtgcgcg aacaggagcg
     1741 aaccgtcgac tgcaccgctg accaggtcgt ggcctgctgg ggcgaacgca accgcattga
     1801 ctgccgccgg atggacgatc gtgcgaagga gctggccgct gggaagcgcg tagacctcca
     1861 ccacgttgcc acgggcaatc gcggctcggt cgccggtagc tgtcaacgct ggcaatgccg
     1921 aggagtagtt tccctcgacg ggagtcacgc ctggcagctc cgcgagcagc tcgctgcgcg
     1981 cggtgtccca gacgtgggta ccgtggtccc tgcagctaag cgcaatgaac cgctgatcgg
     2041 ggacaagact ctcctcggtg tcacactccg cgccgatctg cggcgaatcc caccggcgat
     2101 acggcggggc cgcatcccac acccgggcgg taccgtcgaa cgacgcgccg acgacgcgcc
     2161 gcgactccga atcgaagtga gccgcgaaga tgaggccctt ggggccttcc agcctggcaa
     2221 ccggcatccc cgttgtgacg ttcgagacga caaccgcgcc gtctgcgccg gcagagagga
     2281 tgaggtcgcc agatatggag aactctactg catagatctt gtcgtgatgc gaattgaact
     2341 gggcacgcaa gccccccgac gctgcatccc acacctgttc cgtgccatca cgactcgccg
     2401 ccgctatgag cacgccatcg cgagagaacg cgacggcatc gaccgacgca cccgcatcgc
     2461 gcaaggatcg aacgtgcaca ctaccgggga gctcccagat cgaagcaacg ccgtcatacg
     2521 ttccaaccgc aagatgcgag ccggctggag cgaagctc
//
LOCUS       DASVOR010000716         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_386636,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000716 DASVOR010000000
VERSION     DASVOR010000716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386636"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF516_14100"
     CDS             <1..322
                     /locus_tag="VF516_14100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108858.1"
                     /translation="PPDAGGPDAGIASFSPGPSPGAEAPRGSASAPTPLSSLAAELKR
                     ACDATLDPGKAASLRTTLPSLIVECWCQPHDAVHAQAAFRRLASQRDRETVRRACKKL
                     RVDL"
     gene            complement(345..1448)
                     /locus_tag="VF516_14105"
     CDS             complement(345..1448)
                     /locus_tag="VF516_14105"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8108859.1"
                     /translation="MSDAAAEVTGTDHRTGTLIVMTRNQYLGADLEPLFSAPDAATFN
                     REVIAALDQIAANRFPERALSLALEIARQQPDAVGFQEVYDFTLDGANGPPPYRDQLS
                     DTLRALALLGQHYVVAAQVQELATRVPVDFNGDGVADAVVGVLDRDVILVRRGLAFTP
                     VPFSATCARPSLDGGPGCNYQVIATAHTPVGDIPVQRGFVGIDLTVRGHAYRFVNTHL
                     EIPDVDPSNPLSPAIQAAQAHELIGVLAATRPAGRNLIVVGDFNSSPEQKVTVVGGVP
                     IVPPYLQMAKAGFTDTWLADPRVRPGFTAHQAPNLRNRRSLLSTRIDLIWSRKPPAAA
                     RVDRVGAEPSSRTLFTHLWPSDHAGVVARLRFW"
     gene            1794..>2069
                     /locus_tag="VF516_14110"
     CDS             1794..>2069
                     /locus_tag="VF516_14110"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEX8108860.1"
                     /translation="MSDARSLRGRRIAILAADGFEKIELSAPLRAMQLAGAKVDVVSL
                     RRGRIRGVNLHEPASRIHVDKTVQEADPAAYDALLIPGGFINPDLLRQ"
BASE COUNT          292 a          706 c          780 g          291 t
ORIGIN      
        1 accgcccgac gcgggtgggc ccgacgccgg catcgcgtcg ttctcgccgg gcccctcgcc
       61 gggcgccgag gccccgcgcg gcagcgcgag cgctccgacc ccgctgtcga gcctcgccgc
      121 cgagctcaag cgcgcgtgcg acgccacgct cgatcccggc aaggccgcgt cgctgcgcac
      181 gacgctgccg tcgctgatcg tcgagtgctg gtgtcagccg cacgatgcgg tccatgccca
      241 ggcggcgttc cgccggctcg ccagccagcg cgatcgcgag accgtgcgcc gtgcctgcaa
      301 gaagctgcgc gtcgatctgt gacccggctg tgaccccgcc gccgctacca gaaccgcagg
      361 cgggcgacga ccccggcgtg atccgacggc cacaggtggg tgaacagcgt gcgcgaggac
      421 ggctcggcgc cgacccggtc gacgcgcgcg gccgccggcg gcttgcgcga ccagatcaga
      481 tcgatgcggg tgctgagcag cgatcgccgg ttccgcagat taggggcctg gtgggcggtg
      541 aagccgggcc ggacccgcgg atcggccagc caggtatcgg tgaacccagc cttggccatc
      601 tggaggtagg gcggcacgat ggggacgccg ccgaccacgg tgaccttctg ctcggggctg
      661 gagttgaagt cgccgaccac gatcaggttg cggccggcag ggcgggtcgc cgcgagcacg
      721 ccgatcagct cgtgcgcctg ggcggcctgg atcgcgggcg acagcgggtt gctcggatcg
      781 acgtcgggga tctcgaggtg ggtgttgacg aagcggtagg catggccgcg caccgtgagg
      841 tcgatgccga cgaagccgcg ctggaccggg atgtcgccga cgggggtgtg cgcggtggcg
      901 atcacctggt agttgcagcc cggtccgccg tcgagcgacg gccgggcgca ggtcgccgag
      961 aacggcaccg gcgtgaacgc caggccccgg cgcaccagga tgacatcgcg atcgagcacg
     1021 ccgaccacgg cgtcggccac gccatcgccg ttgaaatcga ccgggacccg ggtcgcgagc
     1081 tcctggacct gcgcggcgac cacgtagtgc tggccgagca gcgccagcgc gcgcaaggtg
     1141 tccgagagct ggtcgcggta gggcggcggg ccgttggcgc cgtcgagcgt gaagtcatag
     1201 acctcctgga acccgacggc gtcgggctgc tgccgcgcga tctccagggc gagcgagagc
     1261 gcgcgctcgg ggaaccggtt cgcggcgatc tggtcgagcg ccgcgatcac ctcgcggttg
     1321 aacgtggcgg cgtccggcgc gctgaacagc ggctccaggt ccgcaccgag gtactggttg
     1381 cgtgtcatca cgatcagcgt gcccgtccgg tggtcggtgc cggtcacctc ggcggcggca
     1441 tctgacacgt cgggatcgac gggaccgggc gcgtcagcgc aggcgcacag tgcggcgagc
     1501 atcgcgaatg cgaagcgcgg tttttctatc tcccacatca ccaaagcctc ctcgcagcgc
     1561 cactgcccgg atcacgcgcg atctcgtgcc acagcgacct gccaggacgg atgcgtgcat
     1621 ctggtgggcc cggcggtggt gggcgaccgc gcgcggaggc cggtcgcagc ggcgtttctt
     1681 gctgcggttt gccgcacgga gcgcgaccct ctcggcgagc gtgtcgtcag ggcggagcgg
     1741 aggtcgccgt cgcggcctgc cgattgcttc gtatcgggca ggaggttttt cccatgtctg
     1801 acgcgcgaag tctcaggggg cgccggatcg cgatactggc cgcggacggc ttcgagaaga
     1861 tcgagctctc ggcgccgctg cgcgcgatgc agctcgccgg cgcaaaggtg gacgtcgtct
     1921 cgctgcgccg cggccggatc cgcggcgtca acctgcacga gccggcatcc cggatccacg
     1981 tcgacaagac cgtgcaggaa gcggatcccg cggcgtacga cgcgctgttg atcccgggcg
     2041 gtttcatcaa ccccgacctg ttgcggcaa
//
LOCUS       DASVOR010000717         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_65221,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000717 DASVOR010000000
VERSION     DASVOR010000717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65221"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="VF516_14115"
     CDS             <1..948
                     /locus_tag="VF516_14115"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8108861.1"
                     /translation="SVDGTFALDGLAAGSYTVRGRGADDREAIASPVDAGARDVTLRL
                     HALAAIDVRLIGFAGTPAVDAENARGDFQKYHGVVAGDRARIAGLPAGRYLVTAHTAE
                     EIDAATVVVADGAVAEVALTGHGSGHLIATVREFATGAPVGDMRCLVYPTADGLIGGD
                     PAWDYDVAPSTDAQGQVMIDPAPAGQSAVYCHNPVAGQSSARAFVTLGRGERATALLF
                     TVRLVPPGAGDIGVELDQGFPLTVTSVRDDGPAAAAGIRAGDAIVALDGRAIGQLSKG
                     GVLHWIMNQPIGAAIRITVRRGAETRTVRVVVGHSLASP"
     gene            1308..2297
                     /locus_tag="VF516_14120"
     CDS             1308..2297
                     /locus_tag="VF516_14120"
                     /inference="COORDINATES: protein motif:HMM:NF020032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108862.1"
                     /translation="MILILTTPQDGAASRGEALLRARGIETLRVDGAFPSEASLHLRW
                     ERSGACARWLSFRDRRIDLADVRAVWHRRVRIECSPRIRDRRAMEYAVAESKHLLRNL
                     WMDLAAPWVPAPQPISVDAEEKLRQLSLAASLGFEIPPSLVTNEPRQLLAFWREHRGQ
                     VITKLLASPSLRESGLDRTYARFTETMTHRDLAALQSVELCPTFAQAYVPKHVELRIT
                     IVNDRVFAAAIHSQRSAHSRVDWRKYDLARASYERHALPPEIEARCVALTRKLGLRYG
                     AIDMILTPDGRYVFLELNPTGEYSWIEAYTGLPITEAICDLLVDLARSGAAPA"
     gene            2294..3325
                     /locus_tag="VF516_14125"
     CDS             2294..3325
                     /locus_tag="VF516_14125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04500 family putative peptide maturation
                     system protein"
                     /protein_id="HEX8108863.1"
                     /translation="MSDEIPLTSALDCLVHLATENVSPEDAIERIKVLRAEHPGLDWE
                     IVWAEQVFASRFDYDLLIGHPTLGTVSLGLTPGDGVPFPLRGVQRWAEMEVVRVNHRS
                     LSMREVAPMLDPYWGEARIYQRIVDHCIVRRELEVHDVPISDDELQRGVDEFRRQRGL
                     DTPEATERWLTEHGLTLDRLEDWVEHDLRTATLRHRLVGATAEAAFRQDPSAFDELHC
                     AAFFSSDVDTWVSEIRRGAGFFEVAERALAGRRDEERSARSILFETVRRADVEASEPL
                     TVGVFAAQLHGAGPYVTLIRSVSPVTWEEARAEVEGRLFATWLARQRQKAIIEWNWGA
                     GLPKPSGEP"
     gene            3453..3827
                     /locus_tag="VF516_14130"
     CDS             3453..3827
                     /locus_tag="VF516_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108864.1"
                     /translation="MTFARQHPVMSVLGAAGVGLIGGPEIALGVVLGAGVAVLIDRRG
                     GERAVGEVVEASAGDAAEGARHVRQRAREILDGAPEIVKQRARAVVQAVRGQIAPMPE
                     PEQAPTHEASAQGAGPTPSSAV"
     gene            3996..>4232
                     /locus_tag="VF516_14135"
     CDS             3996..>4232
                     /locus_tag="VF516_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108865.1"
                     /translation="MLARDIEASAGGRPGITRVVADPRSGRVLVEYEPGVVLDRELEQ
                     LRRSARRRSARTEPVGPAWQSEWHADDPAAVIARL"
BASE COUNT          623 a         1420 c         1521 g          668 t
ORIGIN      
        1 agcgtcgatg ggacgttcgc gctcgacggg ctggccgccg gcagctacac ggtgcgtggg
       61 cggggcgccg acgatcgcga ggcgatcgcc tcgccggtgg acgccggggc gcgcgacgtc
      121 acgctgcgcc tgcacgcgct cgcggcgatc gacgtccggc tgatcgggtt cgcggggaca
      181 cctgcggtcg acgccgagaa cgcgcgcggc gacttccaga agtatcacgg ggtggtcgcc
      241 ggcgatcgcg cgcggatcgc cgggctgccg gccggccgct acctggtcac cgcgcacacc
      301 gccgaggaga tcgacgcggc gaccgtggtc gtggcagacg gcgcggtcgc cgaggtcgcg
      361 ctgaccggcc atggctcggg tcacctgatc gccacggtcc gtgagttcgc gaccggcgcg
      421 ccggtcggag acatgaggtg cctggtgtat ccgaccgccg acgggctgat cggcggcgac
      481 ccggcgtggg actacgatgt cgcgccgtcg accgacgcgc agggccaggt gatgatcgac
      541 ccggcgcccg ccgggcagag cgcggtgtac tgccacaacc ccgtcgcggg gcagagctcc
      601 gcgcgcgcgt tcgtgaccct gggccgcggt gagcgtgcga ccgcgctgct gttcacggtg
      661 cgcctggttc cgccgggagc cggcgacatc ggcgtggagc tcgaccaggg attcccgctc
      721 accgtcacca gcgtgcgcga tgacgggccg gccgccgccg ccggcatccg cgccggcgat
      781 gcgatcgtcg cgctcgacgg ccgcgcgatc ggccagctgt ccaagggcgg agtgctgcac
      841 tggatcatga accagccgat cggcgcggcg atccgcatca ccgtgcggcg aggcgcggag
      901 acccggacgg tgcgcgtggt ggtcggccac tcgctggcgt cgccgtgatt cggccgccca
      961 cgcgctcgga ggcggggttg cgccgaggtc gacctgacct ggcccgggcg cccgaatccc
     1021 gcttgtccgt tctggcccgc ccgtgataca ttcgctggtc catcgtcacg gtcaagaagg
     1081 ggctccatgg tcaagaagtc aaagcaggtt cgaaccaagc tggccgtcct tccgcgtcat
     1141 ccgcatcatg gccggaccct tccggccagg acgccccggg tcctggcgga cgaccaggag
     1201 ctctcacggg tcctcggggc cctgaccctg cagcacgaga gtccaacgta cgacacgacc
     1261 tttccgagtg acccgaacgg gctggcgaaa aatgatctcg tcgattgatg attctgatcc
     1321 tcacgacacc gcaagacggc gcggctagtc gaggagaggc gcttctgcgg gcgcgcggca
     1381 tcgagacgct gcgcgtcgat ggtgcgtttc ccagcgaagc cagcctccac ctccgatggg
     1441 agcgtagcgg tgcgtgtgct cggtggctgt cgttccgtga ccggcgcatc gacctcgccg
     1501 acgtccgggc cgtgtggcat cgcagggtgc gcatcgagtg ttcaccgcgg attcgcgacc
     1561 ggcgtgcgat ggaatatgca gtcgcggaat cgaagcacct gttgcgaaac ctctggatgg
     1621 atctggcagc gccgtgggtc ccagcgccac aaccgatctc ggtcgatgcc gaagaaaaat
     1681 tgcgccagct ctcacttgcc gcgtcgctcg ggttcgaaat tccgccgagc ttggtgacca
     1741 acgagcctcg gcagcttctg gcgttctggc gtgagcaccg tggtcaggtc atcacgaagc
     1801 tcctggcctc tccttcattg cgcgagagcg gcctcgacag aacctatgcg cgattcacgg
     1861 agaccatgac gcatcgcgac ctcgccgcgc tccaatcggt cgagctgtgc ccgacgttcg
     1921 cgcaggcgta tgtcccgaag cacgtcgagc tgcgcatcac catcgtgaac gatcgcgtct
     1981 ttgccgccgc gatccactcc cagcgctcgg cgcacagtcg cgtcgattgg cgcaagtacg
     2041 atctggctcg cgcgtcctat gaacggcatg cgctgccgcc cgagatcgag gcgcgatgcg
     2101 ttgcgctgac acggaagctg gggctccgtt atggcgcgat cgacatgatc ctgacccctg
     2161 atggtcgtta cgtctttctc gagctcaacc cgacgggcga gtactcgtgg atcgaggcct
     2221 acaccgggct gccgatcacc gaggccatct gcgacctcct cgtcgacctt gcacggtccg
     2281 gagccgcccc ggcgtgagcg acgagatccc tctcacgtcg gcgctcgact gtctcgtcca
     2341 cctggcgacc gagaacgtct cgccggagga cgccatcgag cgcatcaagg tcttgcgagc
     2401 cgagcacccc ggtctcgact gggagatcgt gtgggccgag caggtctttg cgtcacgctt
     2461 cgattacgac ctgctgatcg gtcatccgac gctcgggacc gtgtcgctcg gtctcacgcc
     2521 aggggacggg gtgccattcc cgttgcgcgg ggttcagcgc tgggcagaga tggaggtggt
     2581 gcgcgtcaac catcgctcgc tgtcgatgcg cgaggtggcg cccatgctcg acccatactg
     2641 gggtgaagcg cggatctacc agagaatcgt cgaccattgc atcgtgcggc gcgagctcga
     2701 ggtgcacgac gttccgatct cggacgacga gcttcagcgt ggtgtcgacg agtttcgccg
     2761 tcagcgtggg ctcgacacac ccgaggcgac cgaacgctgg ctgaccgagc atggcctgac
     2821 gctcgaccgg ctcgaagact gggtcgagca tgacctcagg accgcgaccc tgcggcatcg
     2881 cctcgtgggc gcgaccgccg aggccgcgtt tcgccaggac ccctcggcgt tcgatgagct
     2941 tcactgcgcc gcgttcttca gcagcgacgt cgatacgtgg gtctcggaga tccggcgtgg
     3001 ggccggcttc ttcgaggtgg ccgagcgtgc actggcggga cgccgtgacg aggagcgatc
     3061 ggcgcgtagc attctgttcg agaccgtgcg gcgggccgac gtcgaagcct ccgagccgct
     3121 cacggtcggc gtcttcgccg cgcagctcca cggtgccggg ccctacgtga cgctcatccg
     3181 ttccgtttct cccgtgacgt gggaggaggc acgcgcagag gtcgagggac ggctgttcgc
     3241 aacctggctc gcccgccagc ggcagaaggc gatcatcgag tggaactggg gtgccggtct
     3301 ccccaagccg tcgggtgaac cgtgacgatg gtcgcgaacg cgccgcgtgg ccatggatcg
     3361 gtacacgaga tgcaccccgc gtgggcatgc aggacaaccg caacgaatcc aaaccaaccc
     3421 acgccccggg tccccctcgg ctccggaagc tgatgacgtt tgcgcgccag cacccggtga
     3481 tgtcagtgct cggcgcggcg ggagtagggc tgatcggcgg gcccgagatc gcgctcggcg
     3541 tggtgctcgg cgccggggtc gccgtgctga tcgaccggcg cggcggggag cgagccgtcg
     3601 gcgaggtggt ggaggcgagc gccggcgacg cggccgaggg cgcacggcac gtgcgtcagc
     3661 gcgctcggga aatcctcgat ggcgcgcccg agatcgtgaa gcagcgggcc cgcgccgtgg
     3721 tccaggcggt gcgcgggcag atcgcgccga tgcccgagcc cgagcaggca ccgacgcacg
     3781 aggcctcggc gcagggcgca ggaccaactc cgagctctgc ggtctgacga gctccgcggt
     3841 gtgacgcgac gaccgggtcg tcgccgcgca cgaagcttgc ttggcgttcg gtgatgcttt
     3901 cgttgccgga tgatggtgga gccgcggcgc tcgcgcgtca cggtcgtgcg tcgcgacggg
     3961 tccgactgga gatcggagca ctgatcggca atccgatgct cgcccgcgat atcgaggcct
     4021 cggccggcgg ccgtccgggg atcacgcgcg tcgtcgcgga tccgcgttca ggccgcgtgc
     4081 tggtcgagta cgagccgggc gtggtgctcg atcgcgagct cgagcagctg cggcgctcgg
     4141 cgcgccggcg cagcgcgcgg accgagccgg tgggcccggc gtggcagtcc gagtggcacg
     4201 cggacgaccc cgcggcggtg atcgcgcggc tc
//
LOCUS       DASVOR010000718         3955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415435,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000718 DASVOR010000000
VERSION     DASVOR010000718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415435"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1048)
                     /locus_tag="VF516_14140"
     CDS             complement(<1..1048)
                     /locus_tag="VF516_14140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108866.1"
                     /translation="MEPSFRPGTTVLGTYRIDDTLGRQGMYMALRVSHPQLGELVLKM
                     LLPETATSLSVHARFVREAQAALRLRGEHVARLIDVGISSDGVPYLVTEAGRGVDLGA
                     ELSRRGALIPAEAVDYVLQVCDALAEAHAHGIVHRRVRPASVLLTARPDGSLLVKLLD
                     FGVIPPPPAAAPIGTIDPVPRPVPGADAAGSDAAYMAPEQIRWPDDVDARADIWSVGA
                     VLYECLTGQRPFPDPTSGSGRVAMSGAPRPMDPQIPAGLQAAVLRCLETDREARFPSI
                     AALAAAIAPYAHDHRMAGFLVERANRLAQGVYDEADPVAAPGRYQVPEVPPMPAVLAP
                     AVAPGGSRAWTRRRH"
     gene            complement(1189..3570)
                     /gene="lon"
                     /locus_tag="VF516_14145"
     CDS             complement(1189..3570)
                     /gene="lon"
                     /locus_tag="VF516_14145"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX8108867.1"
                     /translation="MPKTLPIVPMRGAVLFPGVSLPITAARPQTLRAIEAAVRDPEHR
                     VLAVAQRSDVDEVTADNLFTIGTIATISSLERGLGGVRVALEGRERGIVMRVTPKDGY
                     LLATVSEATELPPLNPTDATFLALHREVRDRAAELATKRGLPAEAVGQMLNQISEPGQ
                     LADLVAGYLDAPLADRQGLLEALAIEDRLRRVLILVQRQIDVLSAQEAIQSKVKEELG
                     ERQREVYLREQLRAIQKELGEGDEAADSSLAELKAKLDKLPLPDDARREVDREWARLS
                     RIGRESMESQVIRTFLETVAELPWGTVTDDHLDIQEAAKILDEDHHGLRDVKDRVLEF
                     LAVHQLAGAKARKGRILLFAGPPGVGKTSIAKSIARAMGRKYIRISLGGARDEADIRG
                     HRRTYIGAMPGRIIQGMRQAGSKNPVFLLDEVDKLGVSYQGDPASALLEVLDPAQNNS
                     FVDHYLGVPFDLSEVMFIATANFLQNIPAPLLDRMEVVEFAGYTEAEKLAIAKDYLLP
                     RQRDENGLTAEQLEVTDDALRDVITSYTREAGVRQLERELGRLARKVARRIAAHEIER
                     ATVDRGDIRALLGRPRVHPEKAAPEDQIGIATGMYYTPAGGDIMFVEASVMPGKGELI
                     LTGQLGDVMKESARAAWTFARAHAAWLSIDDRVFERDLHVHVPAGAIPKDGPSAGIAM
                     ATAIISALSGRPASHEIAMTGEITLSGRVLPIGGLKEKVLGAVRAGITHIVIPRDNEP
                     DLEDLPADVRDRLTVSPVDALSEALAITLRDAALRDGHLAIAERGTGGARLTH"
     gene            3580..>3955
                     /locus_tag="VF516_14150"
     CDS             3580..>3955
                     /locus_tag="VF516_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108868.1"
                     /translation="MTTVVRPCNLPEVPGGGRGATGRSARADEAPDRATWRQHMWIDL
                     RGVLARLVALAVVMAPGGLAGPAHAAPGEGPPAWANDFTARLEALALLQTLNAELLSH
                     DSATLTLDRWCDAHHLAAPAKVV"
BASE COUNT          518 a         1398 c         1490 g          549 t
ORIGIN      
        1 cgtggcggcg ccgcgtccat gcgcgcgaac cgcccggggc gaccgccggc gccagcaccg
       61 ccggcatcgg cggcacctcc ggaacctgat atcgccccgg cgcggcgacc gggtcggcct
      121 cgtcgtacac gccctgcgcg agccggttcg cgcgctcgac caggaagccc gccatccggt
      181 ggtcatgggc gtacggcgcg atcgcggcgg cgagcgcggc gatcgacgga aaccgcgcct
      241 cgcgatcggt ctcgaggcag cgcagcacgg ccgcctgcag gcccgccggg atctgcggat
      301 ccatcggccg cggcgcgccg ctcatcgcga cccgcccgct gccggacgtc gggtcgggga
      361 acggccgctg gccggtcagg cactcgtaga gcaccgcgcc gaccgaccag atgtcggcgc
      421 gcgcatcgac gtcgtcgggc caccggatct gctccggggc catgtacgcg gcgtcgctcc
      481 cggccgcgtc ggctccgggc accggccggg gcaccggatc gatcgtgccg atcggggcgg
      541 cggcgggcgg tggggggatg accccgaagt cgagcagctt gaccagcagc gagccgtcgg
      601 gtcgcgcggt gagcagcacg ctggccggcc ggacgcggcg atgcacgatc ccgtgcgcgt
      661 gagcctcggc gagcgcgtcg cagacctgga gcacgtagtc gacggcctcg gccgggatca
      721 gcgcgccgcg ccgcgagagc tcggcgccga gatcgactcc gcgcccggcc tcggtcacga
      781 ggtacggcac gccgtccgac gagatcccga cgtcgatcag ccgggccacg tgctcgccgc
      841 gcagccgcag cgccgcctgc gcctcgcgca cgaaccgcgc atgcaccgac aggctggtcg
      901 cggtctccgg cagcagcatc ttgagcacga gctcgccgag ctgcggatgg ctgacccgga
      961 gcgccatgta catgccctgg cggcccagcg tgtcgtcgat ccggtacgtt ccgagcacgg
     1021 tcgtgcccgg gcggaagctc ggctccatcc ggcgtcgagc ctagaggaag ccccgcactc
     1081 gctgcaagcg cgcttgcgcg ggccgcgacg cgagcgagcg cgactcactc cacgtgccgc
     1141 cctgcagcac tcagcaattc gccgagacgg ggtggccgtg cagtccgatc agtgggtgag
     1201 ccgggcgccg cccgtgccgc gctcggcgat cgccaggtgg ccgtcgcgca gcgcggcatc
     1261 gcgcagcgtg atcgcgaggg cttcggacag cgcatcgacc ggcgacacgg tcaggcgatc
     1321 gcggacgtcg gccggcaggt cctcgaggtc gggctcgttg tcgcgcggga tcacgatatg
     1381 ggtgatcccg gcgcgcacgg cgccgagcac cttctccttg agcccgccga tcggcagcac
     1441 gcggcccgac agcgtgatct cgccggtcat cgcgatctcg tggctcgccg gtcgaccgga
     1501 cagcgcggag atgatcgcgg tggccatcgc gatgccggcg gacggaccgt ccttggggat
     1561 cgcaccggcc gggacgtgga cgtggagatc gcgctcgaac acgcggtcgt cgatcgacag
     1621 ccacgcggcg tgggcgcggg cgaacgtcca ggcggcgcgc gccgactcct tcatcacgtc
     1681 gccgagctgg ccggtcagga tcagctcgcc cttgcccggc atgaccgagg cctcgacgaa
     1741 catgatgtcg ccgccggccg gcgtgtagta catgccggtc gcgatgccga tctggtcctc
     1801 gggcgcggcc ttctcggggt ggacgcgcgg ccggccgagc agggcgcgga tgtcgccgcg
     1861 gtcgaccgtg gcgcgctcga tctcgtgcgc cgcgatccgg cgggcgacct tgcgggcgag
     1921 ccggccgagc tcacgctcga gctggcggac gccggcctcg cgggtgtagc tggtgatcac
     1981 gtcgcggagc gcgtcgtcgg tgacctccag ctgctcggcg gtcaggccgt tctcgtcgcg
     2041 ctggcgcggc agcaggtagt ccttggcgat cgcgagcttc tcggcctcgg tgtagccggc
     2101 gaactcgacg acctccatgc ggtcgagcag cggcgccggg atgttctgca ggaagttcgc
     2161 ggtcgcgatg aacatcacct cggacaggtc gaacggcacg ccgaggtagt gatcgacgaa
     2221 cgagttgttc tgcgccgggt cgagcacctc gagcagcgcg ctcgccgggt cgccctggta
     2281 ggacacgccg agcttgtcga cctcgtcgag caggaacacc gggttcttgc tgccggcctg
     2341 cctcataccc tggatgatcc ggccgggcat cgcaccgatg tacgtgcggc ggtggccgcg
     2401 gatgtcggcc tcgtcgcgcg cgccgccgag cgagatccgg atgtacttgc ggcccatcgc
     2461 gcgggcgatc gacttggcga tcgaggtctt gccgacgccg ggcggaccgg cgaacaggag
     2521 gatgcggccc ttgcgcgcct tggcgccggc gagctggtgg acggcgagga actcgagcac
     2581 gcgatccttg acgtcgcgca ggccgtggtg gtcctcgtcg aggatcttgg cggcctcctg
     2641 gatgtcgagg tgatcgtcgg tgacggtgcc ccacggcagc tcggcgacgg tctccaggaa
     2701 ggtccggatc acctgcgact ccatcgactc gcggccgatc cgcgacagcc gcgcccactc
     2761 gcggtcgacc tcgcggcgcg cgtcgtcggg cagcggcagc ttgtcgagct tggccttgag
     2821 ctccgcaagg ctgctgtcgg cggcctcgtc gccctcgccg agctccttct ggatcgcgcg
     2881 cagctgctcg cgcaggtaga cctcgcgctg gcgctcgccg agctcctcct tgaccttgga
     2941 ctggatcgcc tcctgggcgc tcagcacgtc gatctggcgc tggaccagga tcagcacgcg
     3001 gcgcagccgg tcctcgatcg cgagcgcctc gagcaggccc tggcggtcgg cgagcggcgc
     3061 gtcgaggtaa ccggcgacga ggtcggcgag ctggccgggc tcggagatct ggttcagcat
     3121 ctggccgacg gcctcggcgg gcaggccgcg cttggtcgcg agctcggcgg cgcggtcgcg
     3181 gacctcgcgg tggagcgcga ggaacgtcgc gtccgtgggg ttgagcggcg ggagctcggt
     3241 ggcctcggac acggtcgcca gcaggtagcc gtccttgggc gtgactcgca tgacgatgcc
     3301 gcgctcgcgg ccctcgagcg cgacgcgcac gccgcccagg ccgcgctcga gcgaggagat
     3361 cgtcgcgatg gtgccgatcg tgaacaggtt gtcggcggtg acctcgtcga cgtcgctgcg
     3421 ctgcgccacc gcgagcacgc ggtgctccgg gtcgcgcacg gcggcctcga tggcgcgcag
     3481 cgtctgcggg cgcgcagccg tgatcggcag gctgacgccc gggaacagca cggcaccgcg
     3541 catcggtacg atcgggaggg tcttgggcat gatagatcga tgaccacggt ggtgcgcccg
     3601 tgcaacctgc ccgaagtccc gggcggtggt cgcggggcca ccgggcggag cgcccgcgcg
     3661 gacgaggcgc cggaccgagc tacctggagg caacacatgt ggatcgatct gcgaggagtc
     3721 ctggcccggc tggtcgcgct cgcggtcgtc atggcccccg gcggcctcgc cgggccggcg
     3781 cacgcggcgc ccggcgaagg gccaccagca tgggccaatg acttcaccgc gcggctggag
     3841 gcgctcgcgc tgctccagac gctgaacgcc gagctgctca gccacgacag cgcgacgctg
     3901 acgctcgacc gctggtgcga tgcgcatcac ctcgcggcgc cggccaaggt cgtcg
//
LOCUS       DASVOR010000719         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415462,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000719 DASVOR010000000
VERSION     DASVOR010000719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415462"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..676)
                     /locus_tag="VF516_14155"
     CDS             complement(<1..676)
                     /locus_tag="VF516_14155"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108869.1"
                     /translation="MNATPPVESTPHRRGLGLDPRMVSMLLALLAIWIALELLTDGLF
                     LSPRNLYNLSIQASAIAVISCGMVFVIVARQIDLSVGSLLAFTGMLATFVQVNWLEGT
                     PAGWLISIAVGIGAGVVVGLFQGWWVGYRGLPALIVTLAGFLMYRGAAFLVADGQTIA
                     PLDETYQVLGGGPAGSLGVPASWAVGAVGCAWLAWQVWSTRRERARYTADQGPAWLDV
                     VKVVAGV"
     gene            complement(698..1756)
                     /gene="xylF"
                     /locus_tag="VF516_14160"
     CDS             complement(698..1756)
                     /gene="xylF"
                     /locus_tag="VF516_14160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_function: GO:0033222 - xylose binding [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8108870.1"
                     /translation="MTLSFGLGPGRRWTILLATVGILAAARIAAAQTVGVSWSNFQEE
                     RWKRDEAAMKAAIEKAGGKYISADAQGSNEKQVANIEDLVTRGAKVLVVLAWDADAVL
                     PAVKRAQAEGIPVIAYDRLIQDKDVFYLTFDNVEVGRMQAQGVFAKKPAGNYVFIKGA
                     PTDPNADLLHKGQLEVLNGAIKAGKIKVVGEQYTEGWAPENAQKNMEQILTKTGNKVD
                     AVVASNDGTAGGVVAALRGQNMVGIPVSGQDGDIAALNRVALGEQTVSVFKDARKLGE
                     AAGTIAVQLAKGKKPKDIAGTQTWAEGSKKIAMTSMFLKPVPVTSDNLDYVITAGWAT
                     KAQVCKGVAAAKAPKACK"
     gene            1883..>2641
                     /locus_tag="VF516_14165"
     CDS             1883..>2641
                     /locus_tag="VF516_14165"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8108871.1"
                     /translation="MSRVRWGILSTSGFARNKVLPAWRGCRHVELAAIASRDLARARA
                     LAAEFGIARAHGSYDALLADPEVDAVYNPLPNHLHVPWTIKALEAGKHVLCEKPIAMT
                     AAEAETLLAAARRHPRLKVMEAFMYRLHPQWQRTVEIVQAGGVGEPRVVQSLFSYFNA
                     DPGNIRNQADAGGGALMDIGCYAISLARLVFGGEPRRVLGLIDSDPRFATDRLTAGVL
                     DFGGRSSSFTVSTQLQPYQRVHVLGTEGRIELEIP"
BASE COUNT          386 a          942 c          887 g          426 t
ORIGIN      
        1 cgacgccggc gacgaccttg acgacgtcga gccacgccgg tccctggtcg gcggtgtacc
       61 tggcgcgctc gcggcgcgtg ctccacacct gccaggccag ccacgcgcag cccaccgcgc
      121 cgactgccca gctcgccggg acgccgagcg agccggccgg gccgccgccc agcacctggt
      181 aggtctcgtc gagcggcgcg atggtctggc catcggcgac caggaacgcg gcgccgcggt
      241 acatcaggaa cccggcgagc gtgacgatca gcgccgggag gccgcggtag ccgacccacc
      301 agccctggaa cagcccgacc acgacgccgg cgccgatccc gacggcgatg ctgatcagcc
      361 agcccgcggg cgtgccctcg agccagttga cctggacgaa cgtcgccagc atgccggtga
      421 acgccagcag cgagccgacc gacaggtcga tctggcgcgc gacgatcacg aacaccatgc
      481 cgcacgagat caccgcgatc gccgaggcct ggatcgacag gttgtagagg ttgcgcggag
      541 acaggaacag cccgtcggtc agcagctcga gcgcgatcca gatcgcgagc agggccagca
      601 gcatggacac catgcgcggg tccaggccga ggccgcggcg gtgcggcgtc gactcgacgg
      661 ggggcgttgc gttcatcggc gcgacgccgt caccgagcta cttgcaggct ttcggcgcct
      721 tggccgccgc gacccccttg cagacctggg ccttggtcgc ccagccggcg gtgatcacgt
      781 agtcgaggtt gtccgacgtg acggggaccg gcttcaagaa catcgacgtc atcgcgatct
      841 tcttgctgcc ctcggcccac gtctgcgtgc cggcgatgtc cttgggcttc ttgcccttgg
      901 cgagctggac cgcgatcgtg ccggcggcct cgccgagctt gcgcgcatcc ttgaacacgc
      961 tgacggtctg ctcgccgagc gcgacccggt tgagcgcggc gatgtcgccg tcctggcccg
     1021 acaccgggat cccgaccatg ttctggccgc gcagcgcggc gaccacgccg cccgcggtgc
     1081 cgtcgttcga cgccacgacc gcgtcgacct tgttgccggt cttggtcagg atctgctcca
     1141 tgttcttctg cgcgttttcg ggcgcccagc cctccgtgta ctgctcgccc acgaccttga
     1201 tcttgccggc cttgatcgcc ccgttcagca cctcgagctg acccttgtgg agcaggtcgg
     1261 cgttggggtc ggtcggtgcg cccttgatga agacgtagtt gccggccggc ttcttcgcga
     1321 acacgccctg ggcctgcatc cggccgacct cgacgttgtc gaacgtcagg tagaacacgt
     1381 ccttgtcctg gatcaatcgg tcataggcga tcaccgggat gccctcggcc tgggcccgct
     1441 tcaccgcggg cagcaccgcg tcggcgtccc acgcgagcac gaccagcacc ttggcgccgc
     1501 gcgtcaccag gtcctcgatg ttggcgacct gcttctcgtt cgagccctgg gcgtcggcgc
     1561 tgatgtactt gccgcccgcc ttctcgatcg ctgccttcat cgcggcctcg tcgcgcttcc
     1621 agcgctcctc ctggaagttc gaccacgaga ccccgaccgt ctgggccgcg gcgatgcgcg
     1681 cggcggccag gatcccgacg gtggcgagca ggatcgtcca gcgacggccc gggccaaggc
     1741 caaaggatag tgtcatacgt gctctcctcc ggacagcgtg gtttacgatt gtggaatctc
     1801 ggaatcgact gtgggcgccg ctcgccgaac atgtcaaggc tggcggtgat gagaaattga
     1861 attacattac aggagcgtca cgatgtcacg agtcagatgg gggattctca gcacgtccgg
     1921 attcgcgcgc aacaaagtgt tgccggcgtg gcgcggctgc cggcatgtcg agcttgctgc
     1981 gatcgcgtcg cgcgatctgg cccgggcgcg cgcgctcgcc gccgagttcg ggatcgcccg
     2041 ggcccacggc tcgtacgacg cgctgctcgc cgatcccgag gtcgacgcgg tgtacaatcc
     2101 gctgcccaac cacctccacg tgccgtggac gatcaaggcg ctcgaggccg gcaagcacgt
     2161 gctgtgcgag aagccgatcg cgatgaccgc cgcggaggcc gagaccctgc tcgccgccgc
     2221 gcgccgccac ccgcggctca aggtcatgga ggcgttcatg taccgcctcc atccgcagtg
     2281 gcagcgcacg gtggagatcg tccaggccgg cggggtcggc gagccgcgcg tggtgcagtc
     2341 gctgttctcg tacttcaacg ccgatccggg caacatccgc aaccaggccg acgccggcgg
     2401 cggcgcgctg atggacatcg gctgctacgc gatctcgctc gcccggctgg tgttcggcgg
     2461 cgagccgcgc cgcgtgcttg gcctgatcga cagcgatccg cggttcgcga ccgaccggct
     2521 caccgccggg gtgctcgact tcggcgggcg gagctcatcg ttcactgtat cgacccagct
     2581 ccagccctac cagcgcgtcc acgtgctcgg caccgagggc cggatcgagc tcgagatccc
     2641 g
//
LOCUS       DASVOR010000720         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_503678,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000720 DASVOR010000000
VERSION     DASVOR010000720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503678"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1589
                     /locus_tag="VF516_14170"
     CDS             <1..1589
                     /locus_tag="VF516_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108872.1"
                     /translation="ADRGAADRRRDVGPAVVTLARALTGCAAAWLAAGLGLGLGLAGC
                     IDPDYHCTTNHDCDVGEAGRCEVDRRCTAYDAMCSSHRRYRDHSGPASNTCLDDSATP
                     VNLCAAGQPPARRDGCAAAVCKALPSCCDTGWSEACVQQAQLSCSDLVCDTRIAITAS
                     DQAGQSELWNLTWDGTHWAMSPDPRTGVLAWLAPAPGDMQPRLAFLQSSSMTPPAMAE
                     LELDGRSFPLATGTYLEATSVDFDRDGRPTAVLGYNDMNGNPRLGIVKLDDGSSRATA
                     AFASHLSWGDLDLDAFPDGMAAAASPASGNYRLLSNAEATDRNRTIGAVVETSMGSSP
                     TTGSPAVRGFGWIDANRDQVLDVIAYGSEINLHLGQRVGLPDTPQIRIDCDPPAISSA
                     CNGSGGTANTQTAQSFAATAVPEASGAALVIATFPIPATVAPSLHRVELGGAKFGLTQ
                     VSPYTFPSCGASPCPAILAVIARDLDGDHKLDVIAIDSELQVYTALGSQLQLTRAIKL
                     TPTLAMPIFDVRTSVTGAPR"
     gene            complement(1604..>2070)
                     /locus_tag="VF516_14175"
     CDS             complement(1604..>2070)
                     /locus_tag="VF516_14175"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8108873.1"
                     /translation="HTTERVDARDIEHTVWRIDPSRSTVGFEVPVLGGLMKVRGGFDR
                     YDGTLACSGDMLLEALVACAGVTLRAGATALSIPVRGGTVSAEGDLDFRGTLGVAKDA
                     PVGFCAIRLRFELDSDAAPDQLARLIELTERYCVIYQTLRHPPSIALEPVAP"
BASE COUNT          290 a          791 c          713 g          276 t
ORIGIN      
        1 cagccgatcg cggcgccgcg gaccgccggc gtgacgtggg tccagcggtg gtgacgctcg
       61 cccgtgcgct caccgggtgc gctgccgcct ggctcgcggc tggcctcggt ctcggcctcg
      121 gcctcgccgg ctgcatcgac cccgactacc actgcacgac caaccacgac tgcgacgtcg
      181 gcgaggccgg ccgctgcgag gtcgaccggc ggtgcaccgc gtacgatgcg atgtgctcct
      241 cgcaccgccg ctaccgcgat cactcgggcc cggcgtcgaa cacctgcctc gacgactccg
      301 ccacgccggt caacctgtgc gcggcgggcc agccaccggc ccggcgcgac ggctgcgccg
      361 ccgcggtctg caaggcgctg ccgagctgct gcgacaccgg ctggtcggag gcctgcgtgc
      421 agcaggccca gctgtcgtgc agcgacctgg tgtgcgacac ccggatcgcg atcaccgcga
      481 gcgaccaggc ggggcaatcc gagctgtgga acctgacgtg ggacggcacg cactgggcga
      541 tgtcgcccga tccccgcacg ggcgtgctgg cgtggctggc cccggcgccc ggcgatatgc
      601 agccgcggct cgccttcctg cagagctcga gcatgacgcc gccggccatg gccgagctcg
      661 agctcgacgg ccgatcgttc ccgctcgcga ccggcacgta cctggaagcg acctcggtcg
      721 acttcgatcg cgacggccgg ccgaccgccg tgctcggcta caacgacatg aacggcaacc
      781 cgcgcctggg catcgtcaag ctcgacgacg gctcgtcacg tgccaccgcc gcgttcgcct
      841 cccacctgtc gtggggcgac ctcgacctcg acgcgttccc cgacggcatg gcggccgcgg
      901 cgagcccggc atcgggcaac taccggctgc tgtcgaacgc cgaggccacg gatcgcaacc
      961 gcacgatcgg cgccgtggtc gagacctcga tgggcagcag cccgaccacg ggctcgccgg
     1021 cggtgcgcgg cttcggctgg atcgatgcca accgcgacca ggtgctcgac gtgatcgcct
     1081 acggcagcga gatcaacctc cacctcggcc agcgcgtcgg gctgcccgac acgccgcaga
     1141 tccggatcga ctgcgatccc ccggcgatct cgtcggcatg caacggcagc ggcgggacgg
     1201 ccaataccca gactgcccag tcgttcgccg ccacggcggt gcccgaggcc agtggcgccg
     1261 cgctggtgat cgcgacgttc ccgattcccg ccacggtggc gccgtcgctg caccgggtcg
     1321 agctcggcgg cgcgaagttc gggctcaccc aggtgtcgcc gtacacgttc ccctcgtgcg
     1381 gcgcctcgcc gtgcccggcg atcctcgcgg tgatcgcgcg cgatctcgac ggcgatcaca
     1441 agctcgacgt catcgcgatc gacagcgagc tgcaggtcta caccgcgctc ggcagccagc
     1501 tccagctgac ccgcgcgatc aagctgacgc cgacgcttgc catgccgatc ttcgacgtcc
     1561 gcacctcggt caccggcgcg ccgcggtagc ggcgttcacg gcgctacggc gcgaccggct
     1621 ccagcgcgat cgacggcggg tgccgcagcg tctggtagat cacgcagtag cgctcggtca
     1681 gctcgatcag cctggcgagc tggtcgggcg ccgcgtcgct gtcgagctcg aaccgcagcc
     1741 ggatcgcgca gaacccgacc ggcgcgtcct tggcgacgcc gagcgtgcca cggaagtcga
     1801 gatcgccctc ggccgacacc gtgccgccgc gcaccgggat cgacagcgcg gtcgcgcccg
     1861 cgcgcagggt cacaccggcg cacgcgacca gcgcctcgag cagcatgtcg cccgagcacg
     1921 cgagcgtgcc gtcgtagcgg tcgaacccgc cgcggacctt catcaggcct ccgagcaccg
     1981 ggacctcgaa cccgacggtc gagcgcgacg gatcgatgcg ccagacggtg tgctcgatgt
     2041 ctcgcgcgtc gacgcgttcg gtggtgtggg
//
LOCUS       DASVOR010000721         3756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_182570,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000721 DASVOR010000000
VERSION     DASVOR010000721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3756
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182570"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VF516_14180"
     CDS             <1..246
                     /locus_tag="VF516_14180"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX8108874.1"
                     /translation="LGFAGALAEVGLPSAEIPLALFSFNVGIELGQLAFISCVIADGA
                     ALRRLSIPWPRRATAVPAYAIGSLAIYWMIERVTALW"
     gene            563..1216
                     /locus_tag="VF516_14185"
     CDS             563..1216
                     /locus_tag="VF516_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8108875.1"
                     /translation="MITLYGFGNIFPEGRGETKDLRAQWALEETGLPYRVHALDHAGG
                     ELDGDAYSRISPFHQVPVIDDDGFVVAESAAVVLYLAEKAGKLIPSDVQGRTRVVQWC
                     FAAVSTVDPTFVCLDVIEIFDKDAHKLKTEVRKLAGRWLKDVERRLQDREWIACADFT
                     VADIMMACVLRGIRKTDLMEPFPRIKAYYERCLARPAWQRTLSLYAERLGVSVDDIR"
     gene            1369..1623
                     /locus_tag="VF516_14190"
     CDS             1369..1623
                     /locus_tag="VF516_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108876.1"
                     /translation="MPLVFGDASGCDTRRHAVPNVETLHLASRRVVHVAVTRHPTQAW
                     TAQQLRNATMDEEAPAVLLRDRDDKFGPEFDRAAQGVGTK"
     gene            1650..1898
                     /locus_tag="VF516_14195"
     CDS             1650..1898
                     /locus_tag="VF516_14195"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8108877.1"
                     /translation="MNAVAERFVGSANRELLDHVLLIDDQHLADLLRQYQRYFNESRP
                     HQGIEQRVPAGSLTVTDSSKPIAVTSVLGGLHVDYRRA"
     gene            2033..2884
                     /locus_tag="VF516_14200"
     CDS             2033..2884
                     /locus_tag="VF516_14200"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8108878.1"
                     /translation="MYVKVGDIDIFYTIAGVGPTCLVPSLAGTPIYERTFTPALQDVM
                     QLVFVELRGNRTATGEIDALTFDAMVDDLDGVRRALGLGRVAVLGHSAHSILALAYAA
                     RYPEWTSHVLAIAGAPGMSAGVLARRATYWDLVASPERKRILAENQARLTEEVLARLT
                     PSERIIVPYAASGPLYFPDPTYDCTPLWEGHEQISDRLFARFWGPQGQFATFDPEANL
                     PNVVAPVFVAQGVFDFIAPPHAWAGELEKLPNATYRAFERSGHYPQLDERELFGEAVA
                     GWLGRGW"
     gene            complement(2934..3179)
                     /locus_tag="VF516_14205"
     CDS             complement(2934..3179)
                     /locus_tag="VF516_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108879.1"
                     /translation="MRSLPPRDDRRRVRVLPSRDARGRFVSFPTTSAPSWYVLCADDY
                     RIPGEAEVMPMQVSAPGRLVAPISVHRAAPDPLRPSR"
BASE COUNT          606 a         1284 c         1279 g          587 t
ORIGIN      
        1 ctgggcttcg ccggggcgct cgccgaggtc ggcctgccgt cggccgagat cccgctggcg
       61 ctgttctcgt tcaacgtcgg gatcgagctc ggccagctcg cgttcatctc ctgcgtcatc
      121 gcggacggcg ccgcgctccg gcggctgtcg atcccctggc cgcgccgggc caccgcggtc
      181 ccggcctacg cgatcggcag cctggcgatc tactggatga tcgagcgcgt cacggccctg
      241 tggtgacgca ttccattctc cggatctgca caacctggtg gtgccgctgg gtgagaacca
      301 cctgcggcca ggtcggtccc atcgtgtgca gaacgtcgtc cagcgggaaa cgcgagggct
      361 cgcccggctg gtgaccagcc aacgggaacg cgccggtcga gcgccgcgag cggctgggcg
      421 gactgctcca cttctgctac cggctcgccg catgagcccg cgatcgactt ctggcacacg
      481 acggggtcac gcgggcgagg ccgtccgcgg cgtggcacgc tccggcgcgg gccggtgaat
      541 ccggccgccg atcggagacg acatgatcac gctctatggc ttcggaaata tctttcccga
      601 ggggcgcggc gagacgaaag acctccgggc gcagtgggcg ctcgaggaga ccgggctgcc
      661 gtatcgagtc cacgcgctcg accacgccgg cggcgagctc gacggcgacg cctacagccg
      721 gatcagcccc ttccaccagg tgccggtcat cgacgacgac ggcttcgtgg tggccgagtc
      781 ggccgcggtg gtgctctacc tcgccgagaa ggccggcaag ctgatcccaa gcgacgtcca
      841 gggccgcacc cgcgtcgtcc agtggtgctt cgctgcggtc agcaccgtcg atccgacctt
      901 cgtgtgcctc gacgttatcg agatcttcga caaggacgcc cacaagctca agacggaggt
      961 gcgcaagctc gccggccgct ggctcaaaga cgtcgagcgc cgcctccagg accgcgagtg
     1021 gatcgcctgc gccgatttca cggtggccga catcatgatg gcctgcgtcc tgcgcggcat
     1081 ccgcaagacc gacttgatgg aacccttccc gaggatcaag gcctactacg agcgctgtct
     1141 ggcccgcccg gcctggcagc gcaccctcag cctctacgcc gagcgactcg gcgtgagcgt
     1201 cgacgacatc cgctagtact gacgcacctg gcggcgcatc gcggggagat gtcccccgcg
     1261 ggccctcctc aggctgtgcc gtggtgagcc tcggcgccgg ctcggcgggc gaagcccgcc
     1321 tgcgcccaca cgctcggtac ccagacgaac ggcgcgagga ccattcccgt gccgctcgtg
     1381 ttcggcgacg cttccggttg cgacaccagg aggcacgccg tgccgaacgt cgagacttta
     1441 catctggcgt cgcggcgtgt cgtccacgtc gcagtgacgc gccacccgac ccaggcatgg
     1501 acggcgcagc agctgcgcaa cgccacaatg gacgaggaag cgccggccgt tctgctgcgg
     1561 gatcgcgacg acaagttcgg ccctgaattc gaccgagcgg cgcagggcgt cggcacgaag
     1621 tgatccggac ggcggttcgg gcgccgaaca tgaacgcggt cgcggagcgc ttcgtcggca
     1681 gcgcaaaccg cgagctgctc gaccacgtgc tgctcatcga cgaccagcat cttgccgacc
     1741 ttctgcgcca ataccagcgc tacttcaacg agagccgacc tcaccagggc atcgagcaac
     1801 gcgtaccagc gggttccctg acggtcaccg actcgtctaa gccgatcgcg gtcacgagcg
     1861 tgctcggcgg gctccacgtc gactatcgca gggcgtgatg catcgtgatc ctgccggacg
     1921 agatcggtag ccagcacgca cctccgcgca ggggcgtttg cgcggccggg tgccttcccc
     1981 tacgatgtcg gctgatcggc cacggacaac caggacagag gagcggcgcg caatgtacgt
     2041 aaaggtcggc gacatcgaca tcttctacac gatcgccggt gtggggccga cgtgcctcgt
     2101 ccccagcctc gccgggacgc ccatctatga gcggaccttc accccggcgc tccaggacgt
     2161 gatgcaactg gtcttcgtcg agctgcgcgg gaatcgcacc gccaccgggg agatcgatgc
     2221 cctcaccttc gacgcgatgg tcgacgacct ggacggggtg cgacgcgcgc tcggcctggg
     2281 ccgggtcgcg gtgctcgggc actcagccca ctccatcctc gccctggcct atgcggcgcg
     2341 ctatcccgag tggacctccc atgtcctggc gatcgcgggc gcgccgggga tgagtgcggg
     2401 ggtgttggcg cggcgcgcga cgtattggga tctggtcgcc tcgcccgagc gcaagcgcat
     2461 cctggccgag aaccaggcca ggctgacgga ggaggttctg gcgcggctga cgccgagcga
     2521 gcggatcatc gtgccctacg cggcgagcgg gccgctctac ttccccgacc cgacctatga
     2581 ctgcaccccg ctctgggagg ggcacgagca gatctccgac cgtctcttcg cgcgcttctg
     2641 ggggccgcag ggacagttcg ccacgttcga ccctgaagca aatctaccga acgtcgtcgc
     2701 cccggtcttc gtcgcccagg gcgttttcga tttcatcgcg ccgccgcatg cctgggcagg
     2761 tgagctggag aagctgccga acgcgacgta ccgggcgttc gagcgcagcg gtcactaccc
     2821 gcagctggac gaacgggagc tgtttggcga ggcggtggcg ggctggttgg ggcggggttg
     2881 gtaggaggcg caaggcggtg ccgggttgag cccggcaccg cccctgctga gattcaccta
     2941 gagggccgca gcgggtcggg ggctgcccga tgcacgctga tcggcgcgac caaacgcccc
     3001 ggcgcgctca cctgcatcgg catcacctcg gcttcgccgg ggatccggta gtcgtccgcg
     3061 cagagcacgt accagctcgg cgcgctcgtc gtggggaacg agacgaaccg accgcgcgca
     3121 tcgcgcgacg gcagcacccg cacgcgacga cgatcatcgc gcggcggcag cgagcgcacg
     3181 cgcgatgcct cggtcgcggc acccgtgcgc gccacgaacc ggccccgcgc gtcccgcggc
     3241 gggaggatgc gagcccgcgc ggccggggtt gcgatcgtct ccatcatggc caatcgctat
     3301 tgcggagcat gtgccgaatt cgtgagctcc tatctatgtg catgatcgcg agacgagcca
     3361 attgaccaat tcgatctgtt gtcctggagc aacgctgtga attggcggca cagatcacga
     3421 tctgctcgtc gagacaatct gcatttcaat ctccagttct gtgcagcatg cgtaatacgg
     3481 agcttgtaag tcgcaatgcg cgagcgctgg tcaacgcgag cgacttcaag gcgcggtgcc
     3541 tgcgcgtgct cgacgaggtg caggcgaccg gcgtgcggga agctgcacgc acgggttgaa
     3601 aacgggtcct tgcccaaccc acctcgaggt gcgggaacgc taggtgtcgt taccgaacat
     3661 cgtccacggc gatcgagacc tgaaaccccg gacgacctga gggtgggtac tcgtcccaac
     3721 gaggacgcag atgacagcgg aggcagatga gccgcg
//
LOCUS       DASVOR010000722         5876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_503717,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000722 DASVOR010000000
VERSION     DASVOR010000722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5876
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503717"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF516_14210"
     CDS             <1..237
                     /locus_tag="VF516_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108880.1"
                     /translation="AGTRDHNTETTNQLAAFAQQIAQRDPAVDTDTFDRFWYVLNASF
                     WYGLADVARASRLDYGVLDVAEECSTAFFATDPE"
     gene            complement(254..2413)
                     /locus_tag="VF516_14215"
     CDS             complement(254..2413)
                     /locus_tag="VF516_14215"
                     /inference="COORDINATES: protein motif:HMM:NF041121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8108881.1"
                     /translation="MHDGEAGRHPRDRRGRSRAARRCDLVVPVPAACTAEAGSGRGGR
                     WRLVEDATVERIAAGGLWLPVLEPARAPALELVFVIDCDPRLRPWRELMQALIAVVRS
                     SGRLRNVTTWTIDGHALTAQLRRGGGGQPRSARHVVDPTGRTVVLVVSDFAGPLWREG
                     DGLASFQTWARAMPTTLVSLLPERLWDRTVLGGLSQVALSAWQRAQVSAALGCEPAIE
                     DDAVALPVITVDPTSLRAWTALVAGDPRARLTGVAALPDPSVDTRVRAMLRRDLLDGG
                     DAAQAAQRAAQLREELAAAMHAGELEDPVERFQREASPSVIRLAAALVRAPLRLDVMR
                     LVQRAIAPATGPLELAEILGSRLFALRPNGGVREESVWFDIEPTVRAQIAGLGTARDG
                     VRVLEEVGRYVRDHLGGRYDFEALMLDPDSQPGDIPDELRPFAELYVEELGRMGGRYR
                     ELARRLAIATVPRKLRTDTAARLAEPPAPPPAPLETSDAEILQHAANGDLRGALRLLI
                     RRDGTAVYNYCRAALDDKVLGEDVQQQVFLAALRDLPRFSGRTSLRAWLFAIARHRVL
                     DVAKARRRAPREVDEGEAVRAPDPQPLAHERLDDARLREALGQCLQKLPPHILTAVLL
                     RFQQGFTFEEMADVCREKPGTLQAQVARALPALRRCIEDRTGDQILTAHKKPVETFFE
                     KLGRTGDKLSEQQVEAALEQLGGAGGKPPGPGPSGAQ"
     gene            complement(2406..3404)
                     /locus_tag="VF516_14220"
     CDS             complement(2406..3404)
                     /locus_tag="VF516_14220"
                     /inference="COORDINATES: protein motif:HMM:NF019348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8108882.1"
                     /translation="MTEASASTSWHIYQGTNAARPGGPPRIPPPPPWRTPKARPGSAI
                     AVTEMEADFQPFLADDETRHMVNAALLLRRPLLVTGNPGTGKTRLAYAVAHELNLGPV
                     LRWSINTSSTLRDGLYDYDAIGRLQATAPGADGAPARASALGDFLTLGPLGTALLPWD
                     RPRVVLVDEIDKSHVDLPNDLLHVFEDGQFPIRELLRAESAERSATVIGHGRVAIPDG
                     IVRCSEFPLVIMSSNGEREFPAAFRRRCLELHVKPPSAERLGEIIAEHFSKGRFAGGL
                     PDGWQRLCKEFVERRDRDALATDQLMNAIYLRTCGVGKDPVWDQVLASVWHALSDA"
     gene            complement(3401..4735)
                     /locus_tag="VF516_14225"
     CDS             complement(3401..4735)
                     /locus_tag="VF516_14225"
                     /inference="COORDINATES: protein motif:HMM:NF039783.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108883.1"
                     /translation="MRRDPPRGSTLSPGRRAQLTAALVGAFQNSIEELRELASYYDAG
                     LAVNLPGANASVKAVILGVVDMSCDCGESIVESVARSARRRRPHNRALAVAVSETWPF
                     ADGLDYEYVDELVRQVRAAGISVEQLRRATRSVLGAGRPPLWDARGPEPVADLIEGLA
                     QLGGHPPRLALVAHQLCSSGRVCEGIAGWLQRIAPDLVAAGAATADHDARMLLLLRRG
                     VDDRYKGEIYFSEPDGRAKFVAELRDQVALDGVPAMFAATVADSEDLRAALLHAELAR
                     VEVMLPVRDLLAPVERWDLFGSGPIGELYPVVVRPLERALAMQHHEYVGAANLWRSAW
                     KAVRRAPVMRQLELDPAQVEAAVRAHPKRWCVAGFRCAAPSRPDHLAALERLVKRGVA
                     AMLALRHDGSWPKHLQADLTLPGAVHDHRCDRPEHPVMLLWDDVDFIPGSRR"
     gene            complement(4934..5782)
                     /locus_tag="VF516_14230"
     CDS             complement(4934..5782)
                     /locus_tag="VF516_14230"
                     /inference="COORDINATES: protein motif:HMM:NF039430.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector-associated domain EAD1-containing
                     protein"
                     /protein_id="HEX8108884.1"
                     /translation="MLSPGRRAQLTAALLGAFQNSIDDLRELASYYEAEGLAANLPGA
                     NASVKAVIRAVVDVSCDCGEAIVESVARSARRRRPHNQALATVVGETWPFADGLDHEC
                     VDELIRQVHAAGLSLEQLRQATQSVLGAVRPPLWDAHGSEPSEDLIEGLAQLRGRPLK
                     LALVARRLGGAHGLPEGIARWLQRVAPDLVDPVPEVGSAGAASCDHVAAAPNDVRLYD
                     RLSRLPDTVFEQIVFYARIDRSLIAPPSAPLATRMLDVAKLAAVDQDLCRRLAAELDQ
                     RAPWTR"
BASE COUNT          884 a         2150 c         1995 g          847 t
ORIGIN      
        1 gccggcaccc gcgatcacaa caccgagacc accaaccagc tcgcggcgtt cgcccagcag
       61 atcgcgcagc gcgatcccgc ggtcgacacc gacaccttcg accggttctg gtacgtgctc
      121 aacgcgagct tctggtacgg cttggccgac gtcgcgcgcg cgtcgcgcct cgactacggc
      181 gtgctcgacg tggccgagga gtgctcgacc gcgttcttcg cgaccgaccc cgagtgatca
      241 cgccgcagcg cggctattgt gcgccagacg ggccagggcc aggcggcttg ccgccagcgc
      301 cgcccagctg ttcgagcgca gcctctacct gttgctcgga cagcttgtcc cccgtgcggc
      361 ccagcttttc aaagaaggtc tccaccggct tcttgtgggc ggtcaatatc tggtcgccgg
      421 tcctgtcctc gatacaacga cggagggccg gcaacgcacg agcgacctgt gcctgcaggg
      481 tgcccggctt ttcccggcaa acgtcagcca tctcctcgaa tgtgaatccc tgctggaagc
      541 gcaagagcac cgcggtcagg atatggggcg ggagtttttg gaggcactgc cccagagcct
      601 cacgcaagcg agcatcatcg aggcgctcgt gggccagcgg ctgcggatcc ggcgcgcgca
      661 cggcctcgcc ctcatcgacc tcgcgcggtg cccttcggcg agccttggct acatcgagga
      721 cccggtgacg agcaatggcg aacagccacg cgcgcaacga agtcctgccg gagaaccgcg
      781 gcagatcgcg caacgcggcg agaaacacct gttgttgtac atcctcgccc agtaccttat
      841 cgtcgagggc agcgcggcag tagttataca cggcagtgcc atcgcgtcgt atcagcagtc
      901 tcagagcgcc tctgaggtca ccgttcgcgg catgctggag gatctccgcg tccgacgtct
      961 cgagcggggc gggaggcggc gcaggcggtt cggcaagcct ggcagccgta tcggtccgga
     1021 gcttgcgcgg cacagtggcg atcgccaggc gccgggcgag ctcgcgatac cggccaccca
     1081 tgcggccgag ctcctcgacg taaagctcgg cgaacggccg cagctcatcc gggatatcgc
     1141 cgggctggct gtccggatcg agcatcagcg cctcgaagtc ataacgaccg cctaggtgat
     1201 cgcgcacgta gcggccgacc tcctccagca cccgcacgcc gtcgcgcgcc gtccccagac
     1261 ccgcgatctg cgcgcgcaca gtgggctcga tatcgaacca caccgactcc tcccgcaccc
     1321 cgccgttggg ccgcagggcg aacagccgac tgcccaggat ctcggcgagc tccagcggcc
     1381 ccgtggccgg cgcgatcgcg cgctggacca gccgcatcac gtcgagccgt aacggggccc
     1441 gcaccagcgc tgccgcgagc cggatcacgc tggggctggc ttcgcgctgg aaccgctcga
     1501 ccgggtcctc gagctctccg gcgtgcatgg cggcggccag ctcttcgcgc aactgcgcgg
     1561 cgcgctgggc cgcctgtgca gcgtcgccgc catcgagcag gtcgcgccgg agcattgcgc
     1621 gaacgcgggt gtccacggac gggtctggca gcgcggcaac gccggtcaac cgtgcgcggg
     1681 ggtcgccggc gaccagcgct gtccaggcgc gcagtgaggt cggatcgacc gtgatgaccg
     1741 gcagcgccac cgcgtcatcc tcgatcgcgg gttcgcatcc cagcgccgcg ctgacctggg
     1801 cgcgctgcca cgcggacagt gcgacctgcg acaggccgcc gagcaccgtg cgatcccaca
     1861 gccgctcagg cagcagcgag accagcgtcg tcggcatcgc gcgggcccac gtctgaaagc
     1921 tggccaggcc gtcgccctcg cgccacagcg gccctgcaaa gtcggagacc acgagcacga
     1981 cggtccggcc agtgggatcc acgacgtggc gcgcgctgcg cggctggcct ccgccgccgc
     2041 ggcgcagctg cgcggtcagc gcgtggccgt cgatcgtcca cgtcgtgacg ttgcgcagcc
     2101 ggcccgacga gcgcaccacc gcgatcagcg cctgcatcag ctcgcgccac ggtcgcagcc
     2161 ggggatcgca gtcgatcacg aacaccagct cgagcgccgg cgcgcgcgcg ggctcgagca
     2221 ccggcagcca cagcccgccc gcggcgatgc gctccaccgt ggcgtcctcg accaggcgcc
     2281 accgaccccc gcggccggat ccggcctcag ccgtgcatgc ggcaggtact gggacaacca
     2341 gatcgcatcg gcgagctgct ctagatcgac cgcgccggtc gcgaggatgg cgcccagctt
     2401 ctccatcatg catcgctcag cgcgtgccac acgcttgcca ggacctggtc ccagaccgga
     2461 tccttcccga cgccgcaggt ccggagatag atcgcgttca tcagctgatc ggtcgcgagc
     2521 gcgtcgcgat cgcgccgctc gacgaactcc ttgcacagcc gctgccagcc gtcgggcaac
     2581 ccgcccgcga accgaccctt gctgaagtgc tccgcgatga tctcgcccag ccgctccgcg
     2641 ctcggcggct tgacatggag ctcgaggcag cgccggcgaa acgccgccgg gaactcgcgc
     2701 tcgccattgc tgctcatgat gacgagcgga aactccgagc agcgcacgat accgtccggg
     2761 atcgcgaccc tgccgtggcc gatcacggtc gccgaccgct cggcggactc ggcccgcaac
     2821 agctcgcgga tcgggaactg tccgtcctcg aagacgtgca gcagatcgtt cggcaaatcg
     2881 acgtggctct tgtcgatctc gtcgaccagc actacacgcg ggcgatccca cggcaacagc
     2941 gcggtaccga gtggaccgag cgtgaggaag tcgccgagcg cactcgcccg ggcgggagcc
     3001 ccgtcggcgc cgggcgccgt cgcctggagc cggccgatcg cgtcgtagtc atagagccca
     3061 tcgcgcagcg tcgagctggt gttgatcgac cagcgcagga ccggcccgag gttgagctcg
     3121 tgcgcgaccg cgtacgccag gcgcgtcttg cccgttcccg ggttgccggt gaccagcagc
     3181 ggccggcgca gcagcagcgc cgcgttgacc atgtgccggg tctcgtcgtc ggccaggaat
     3241 ggctggaagt ccgcctccat ctcggttacc gcgatcgccg accccggcct ggccttgggc
     3301 gtccgccagg gcggcggcgg cggaatgcgc ggtgggccgc cgggccgcgc ggcattggtg
     3361 ccctggtaaa tgtgccacga cgtcgacgca ctcgcttcgg tcatctccgg ctccctggaa
     3421 tgaagtccac atcgtcccac agcagcatca cgggatgctc cgggcggtcg catcggtgat
     3481 cgtgcacggc gcccggcagc gtgaggtcgg cctggaggtg cttgggccac gagccgtcgt
     3541 ggcgcagcgc gagcatcgcc gcgacgccgc gcttcacgag gcgctccaac gcggccagat
     3601 gatccggccg gctcggcgcc gcgcagcgga atccggcgac gcaccatcgc ttcgggtgcg
     3661 cccggaccgc ggcttcaacc tgtgccggat cgagctccag ctggcgcatc actggagctc
     3721 gccgcacggc cttccaggcg ctgcgccaca ggttcgcggc cccgacgtac tcgtgatgct
     3781 gcatcgcgag cgcgcgctcc agtggccgca cgaccacggg gtacagctct ccgatcggcc
     3841 cgctgccgaa caggtcccag cgctcgaccg gtgcgagcag atcgcggacc ggaagcatga
     3901 cctcgacccg tgcaagctcg gcatggagca gcgcggcgcg tagatcctcg ctgtccgcga
     3961 ccgtggccgc gaacatcgcc ggcaccccgt cgagcgccac ctgatctcgg agctcggcca
     4021 cgaactttgc gcgtccgtcg ggctccgaga agtagatctc gcccttgtag cgatcgtcga
     4081 cgccgcggcg cagcagcagc agcatccgcg catcgtgatc tgccgtcgct gcgccggcgg
     4141 ccaccaggtc cggcgcgatg cgctgcagcc agccggcgat gccttcgcag acacggccgg
     4201 agctgcagag ctggtgcgcg actagcgcga gcctcggcgg atgcccaccg agctgcgcga
     4261 gcccctctat caagtccgcg acgggctcgg ggccacgcgc gtcccacagc ggcggacgcc
     4321 cggcgccgag cacgctccgg gtggctcgcc gcagctgctc gacggatatt ccggcggcac
     4381 ggacctgccg gaccagctcg tcgacatact cgtagtcgag cccgtcggca aacggccagg
     4441 tctcgctgac cgcgacggcc agtgcccggt tgtgcggccg ccttcgccgt gcgctgcgag
     4501 ccacggactc aacgatcgac tcaccgcagt cgcatgacat gtccacgacg ccgaggatca
     4561 ccgccttgac cgatgcgttc gctccgggca ggttcaccgc cagcccggcg tcgtagtacg
     4621 acgcgagctc gcgtagctcc tcgatgctgt tctgaaacgc accgaccagc gccgccgtga
     4681 gctgtgcgcg gcgcccgggg ctcagcgtgg atccccgggg cgggtcacgt cgcatcgccc
     4741 ggctacgcga tggcttcgac atacgatcgc acgctgggat gattcgcgct ccgccagatc
     4801 gagatcccgt ggttgtgtcc gtgtacttga cagacccagg tcgcgacggc gtatccgcga
     4861 tggaacaact gattcgagtc gtggagctgg cggttgggcc cgaaccacgc ggcgaccgga
     4921 tctcccgagg ctgctaccgc gtccatggcg cccgctggtc gagctcggcg gcgagccgtc
     4981 ggcacagatc ctggtcgacc gccgcgagct tcgcgacgtc gagcatgcgc gtggcgagcg
     5041 gtgcgctcgg cggcgcgatc aggctgcgat cgatccgcgc gtagaacacg atctgctcga
     5101 agaccgtgtc cgggagccgt gacagccgat cgtacaggcg cacgtcgttg ggggcggccg
     5161 cgacgtggtc gcagctggcc gcgcctgcgc tgccgacctc cggcacggga tccactagat
     5221 ctggtgcgac gcgctgcagc cagcgggcga tgccctcggg gagaccgtgg gcaccgccga
     5281 gccggcgtgc gaccagcgcg agcttcagcg gtcgtccgcg gagctgcgcg agcccttcga
     5341 tcagatcctc cgagggctcg gagccgtgcg cgtcccacag cggcggacgc actgcaccga
     5401 gcacgctctg ggtggcttgt cgcagctgtt cgagcgacag ccctgcggca tggacctgcc
     5461 ggatcagctc gtcgacgcat tcgtggtcga gcccgtcggc gaacggccag gtctcgccga
     5521 ccacggtggc cagcgcctgg ttgtgcggcc gccttcgccg cgcgctgcgc gccacggact
     5581 cgacgatcgc ctcgccgcag tcgcacgaca cgtccacgac cgcgcggatc accgccttga
     5641 ccgacgcgtt cgctccgggc aggttcgccg ccagcccctc ggcctcgtag tacgacgcga
     5701 gctcgcggag gtcgtcgatg ctgttctgga aggcaccgag cagcgcggcg gtgagctgcg
     5761 cgcggcgtcc ggggctcagc atgggccccc gggcgggctg ttcggcgtga acgctctcac
     5821 catcgcgccg cgcaacccac gcttgtcctc cagcttcaag cgcatcgccg ccatgt
//
LOCUS       DASVOR010000723         2872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_328394,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000723 DASVOR010000000
VERSION     DASVOR010000723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328394"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1286)
                     /locus_tag="VF516_14235"
     CDS             complement(<1..1286)
                     /locus_tag="VF516_14235"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX8108885.1"
                     /translation="MPPLTRAEATSYKETSLHAHVMEFIAGLAARNDPRLAVSSFGTR
                     PGGRDLPLCVLSAHGVRTPDEARRRGLPVVLIINGIHAGEVEGKESSMMLMRDLLDGQ
                     NADLLGELTLVVVPLFNPDGNDAVDPANRRLDLPRLHGQIGPEKVGTRVTASGINLNR
                     DYLRQAAPEMRLLQSRVCQVWQPELTIDCHATNGSVHRYAMTYDVPHTVATGRAEPIA
                     YMRTKMMPAVTAALARDHQLLAGWYGNFVEDERSLDAQLDADPTAAVTEGWMTYTHHP
                     RFGSNYRGLTNRLDLLLECYSYLPFADRVRTTYACLLEALRYTAGHAGEIVQLVAASR
                     APRDRIAIRYRLDAFHDPIEVPTRTPRTLDGAPSMARLRYFANFAGANVVDRPPAYLV
                     DAAVAEHLVRHGLAVEPAPAHVDAEVATIAEIASEGG"
     gene            complement(1343..1582)
                     /gene="minE"
                     /locus_tag="VF516_14240"
     CDS             complement(1343..1582)
                     /gene="minE"
                     /locus_tag="VF516_14240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEX8108886.1"
                     /translation="MFRGKPSSREVAKGRLHLVLAHDRVGLEGGKLQEMRREIADVIS
                     RYVPIDLDAVEIQIESKSRATELRVSSPLPARGSG"
     gene            complement(1619..2422)
                     /gene="minD"
                     /locus_tag="VF516_14245"
     CDS             complement(1619..2422)
                     /gene="minD"
                     /locus_tag="VF516_14245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEX8108887.1"
                     /translation="MGRAIVITSGKGGVGKTTTTANLGAAIAQLGMSVVLIDADIGLR
                     NLDVVMGLENRIVYHLVDVVQRKCTAGKALIKDRRLDNLWLLPASQTDDKEAVTPADM
                     RKLVGELKAAYDYVLIDCPAGIEQGFKNAIAGADEAVVVATPEVSSIRDADRVVGLLA
                     AQDIPTRLIVNRISAQMVKKGDMLSQSDVIEILALELIGAVPLDEHVVASTNKGMPAV
                     LDGKSSAAREFARIARRVIGLDTETERETGGGWFSRIGRALGFSSATAA"
     gene            complement(2431..2853)
                     /locus_tag="VF516_14250"
     CDS             complement(2431..2853)
                     /locus_tag="VF516_14250"
                     /inference="COORDINATES: protein motif:HMM:NF015716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEX8108888.1"
                     /translation="MLAAVGARDAAAAGDDESADETPVGPRLVVGPVRSGRIVDHVGH
                     LIVVGDVNPGAAVRAEGSLIVLGRLRGVAHAAIGRDSGCIIALSLQPQQLRIGRMVAR
                     AGDADRPSGGAEIAYVTGETIVVERFSGRLPSGLAASM"
BASE COUNT          391 a         1046 c         1000 g          435 t
ORIGIN      
        1 ccgccctcgc tcgcgatctc ggcgatcgtg gcgacctcgg cgtcgacgtg ggccggcgcc
       61 ggctcgaccg cgaggccgtg gcgcaccagg tgctcggcga ccgcggcgtc gaccaggtac
      121 gcgggcggcc ggtcgaccac attggccccg gcgaagttgg cgaagtagcg cagccgcgcc
      181 atcgacggtg cgccgtcgag cgtccgcgga gtgcgggtcg gcacctcgat cggatcgtgg
      241 aacgcgtcga gccggtagcg gatcgcgatg cggtcgcgcg gcgcgcggct ggccgcgacc
      301 agctgcacga tctcgcctgc atggcccgcg gtgtagcgca gcgcctcgag caggcaggcg
      361 taggtcgtcc gcacccggtc ggcgaacggc aggtaggagt agcactcgag cagcaggtcg
      421 agccggttgg tcaggccgcg gtagttgctg ccgaaccgcg ggtgatgggt gtaggtcatc
      481 cagccctcgg tcaccgccgc ggtcgggtcg gcgtcgagct gggcgtcgag cgagcgctcg
      541 tcctcgacga agttgccgta ccagccggcg agcagctggt gatcgcgggc gagcgccgcg
      601 gtgaccgccg gcatcatctt cgtgcgcatg tacgcgatcg gctccgcgcg cccggtcgcc
      661 acggtgtgcg gcacgtcgta ggtcatcgcg tagcggtgga ccgagccgtt ggtcgcgtgg
      721 cagtcgatcg tcagctcggg ctgccagacc tggcagaccc gcgactgcaa cagccgcatc
      781 tccggcgccg cctgccgcag gtaatcgcgg ttgaggttga tgcccgaggc ggtcacccgc
      841 gtgccgacct tctcggggcc gatctggccg tgcagcctgg gcaggtcgag ccggcggttc
      901 gccggatcca ccgcgtcgtt gccgtcgggg ttgaacagcg gcacgaccac cagcgtcagc
      961 tcgccgagca gatcggcgtt ctggccgtcg agcaggtcgc gcatcagcat catgctcgac
     1021 tccttgccct cgacctcgcc ggcgtggatc ccgttgatga tcagcacgac cggcaggccg
     1081 cgccgccgcg cctcgtcggg cgtgcgcacg ccgtgcgccg acagcacgca cagcggcagg
     1141 tcgcggccgc ccggacgggt gccgaacgac gacaccgcga gccgcgggtc gttgcgcgcc
     1201 gcgagccccg cgatgaactc catcacgtgg gcgtggagcg aggtctcctt gtacgaggtg
     1261 gcttcggcgc gcgtcagggg tggcatcgcg gcatcgtgtc acgccggccc ggcgattgcg
     1321 cgccgcaatc gccgcgatgc gatcagccgc tgccgcgcgc cgggagcggg ctcgacaccc
     1381 ggagctcggt cgcgcggctc ttgctctcga tctggatctc gaccgcgtcg agatcgatcg
     1441 gcacgtagcg cgagatcacg tcggcgatct cgcgccgcat ctcctgcagc ttgccgccct
     1501 ccagcccgac ccggtcgtgg gccaggacca ggtggaggcg gcccttggcg acctcccggc
     1561 tcgatggctt cccgcgaaac atgttccaca taaggactcc gttcgtggtc gcggggccct
     1621 aggccgcggt tgccgacgag aaccccagcg cgcggccgat ccgcgagaac cacccgccgc
     1681 cggtctcgcg ctcggtctcg gtgtccaggc cgatcacccg ccgcgcgatc cgcgcgaact
     1741 cgcgcgccgc gctcgacttg ccgtcgagca ccgccggcat gcccttgttg gtcgaggcca
     1801 cgacgtgctc gtcgagcggc accgcgccga tcagctcgag cgcgaggatc tcgatcacgt
     1861 cgctctgcga cagcatgtcg cccttcttga ccatctgcgc cgagatccgg ttgacgatca
     1921 gccgggtcgg gatgtcctgg gccgcgagca ggccgaccac ccgatcggcg tcgcggatcg
     1981 acgacacctc gggcgtcgcg accaccaccg cctcgtcggc tccggcgatc gcgttcttga
     2041 acccctgctc gatccccgcc gggcagtcga tcagcacgta gtcgtacgcc gccttgagct
     2101 cgccgaccag cttgcgcatg tcggccggcg tcaccgcctc cttgtcatcg gtctgcgacg
     2161 ccggcaagag ccacaggttg tcgagccggc ggtccttgat cagcgccttg ccggccgtgc
     2221 acttgcgctg caccacgtcg accaggtggt agacgatccg gttctcgagg cccatcacga
     2281 cgtcgaggtt gcgcagaccg atgtcggcat cgatcagcac cacgctcatc ccgagctgtg
     2341 cgatcgcggc cccgaggttg gcagtcgtgg tggtcttgcc gaccccaccc ttgcccgaag
     2401 tgatgacgat tgctcgaccc atgttgtctc ctacatgctt gcggcaaggc cgctgggaag
     2461 tcgtccagag aaccgctcga ccacgatggt ctcgccggtg acatacgcga tctcggcacc
     2521 gccactgggg cgatccgcat cgccggctcg ggcgaccatg cgcccgatcc tgagctgttg
     2581 cggctgcagc gacagcgcga tgatgcagcc ggagtcgcgg ccgatcgccg cgtgcgccac
     2641 cccgcgcagc cggccgagca cgatgaggct gccctcggcg cgcaccgcgg cccccgggtt
     2701 gacgtcgccg acgacgatca ggtgcccgac gtgatcgacg atcctccccg agcgcacggg
     2761 gccgaccacc agccgcggcc cgacgggcgt ctcgtcggcg gattcgtcat caccggccgc
     2821 tgccgcgtcg cgcgcgccga ccgccgcgag caccgccgcc agcgccgcga cc
//
LOCUS       DASVOR010000724         3129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_65329,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000724 DASVOR010000000
VERSION     DASVOR010000724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65329"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VF516_14255"
     CDS             complement(<1..192)
                     /locus_tag="VF516_14255"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX8108889.1"
                     /translation="MADNSGVNLVLAAEIAAAHAAGRPLVALESTILAHGLPWPDNLG
                     IARELEAAVRAGGAVPATVA"
     gene            269..424
                     /locus_tag="VF516_14260"
     CDS             269..424
                     /locus_tag="VF516_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108890.1"
                     /translation="MSSTTRLLVLGVVKLFQPVHGYEVRRELLSWRAQEWASVQLGSI
                     YNALKTG"
     gene            complement(757..972)
                     /locus_tag="VF516_14265"
     CDS             complement(757..972)
                     /locus_tag="VF516_14265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108891.1"
                     /translation="MKFRLTEQLLDEARRFALRSACRDCFYWRPERGACAHDWPDEGQ
                     RRWPLDAPNPDGSPPTEAAFCKEFELR"
     gene            complement(969..1223)
                     /locus_tag="VF516_14270"
     CDS             complement(969..1223)
                     /locus_tag="VF516_14270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108892.1"
                     /translation="MIRFLVYLAIVIALAWFATTVQLGKHTLVGHIRAIWHTEEVRDL
                     KDGVKERARPAVERLERGIHATTGSGREAAAHGSGAEASQ"
     gene            complement(1282..>3129)
                     /locus_tag="VF516_14275"
     CDS             complement(1282..>3129)
                     /locus_tag="VF516_14275"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8108893.1"
                     /translation="CVDAAVLGGLGAVWLSALAVAEPAGNIDLNAFRPAMDSRGYLTV
                     NASQVLGDKEISFGLGALDWGHSLLKLDGMGHTYSIDDMIGATLIGAFGIHAGPAELE
                     FGASVPFLIMSGDRGPDVGQNEFKLDGQGIGNIGVHFKTRFLKTSRGPHIGLGVIASL
                     YLPTTNPTDRFLGEASRSNGTTGGKLVPQVMGILDKEFGRTGALRMSLNAGIRIRSAE
                     TFTNSDPGIDNAPVTNGTVTAGSEIPFGFGIAYALSRQKFDLVGEVIGSIPLGDHTNY
                     QPLEVLGGVKLYLARNSFLSLGVGRGLVPDKGANPGVRAMIGIVFEPNIGDRDGDGIK
                     DDVDKCPDEPEDFDGFEDEDGCPDPDNDHDGVPDVDDRCPDIPGPKENDGCPITANND
                     RDGDGIPDNVDKCPDQPEDKDGFQDEDGCPDPDNDGDGIPDNVDLCPNDPEDKDGFED
                     ADGCPDPDNDHDRIPDKDDKCPNEPETYNGYQDEDGCPDRGRVVVTDTSIEILDVIYF
                     EYDKAVILPKSFPILDAVAATLQGNPSIQLVEIQGHTDERGDDTYNLELSDRRAKAVM
                     KYLVDKGVDPKRLTAHGYGETMPLDNRHNEAAWAKNRRVAFLIIKRTND"
BASE COUNT          412 a         1037 c         1138 g          542 t
ORIGIN      
        1 ggcgaccgtc gccgggaccg cgccgcccgc gcgcaccgcc gcctcgagct cgcgcgcgat
       61 ccccaggttg tcgggccacg gcaggccgtg cgcgaggatc gtcgactcca gcgcgaccag
      121 cgggcggccc gcggcgtgcg ccgccgcgat ctcggctgcg agcaccaggt tcacgccaga
      181 gttatccgcc atcgcgcggg cggccggtag tcgtttcgtc acccggccgc cggctgcaca
      241 gcggccggcc gccgtgctac gaggaattgt gtcgtccacg acccgcctgc tcgtgctcgg
      301 cgtggtcaag ctgttccagc cggtccacgg ctatgaggtg cgccgcgagc tgctcagctg
      361 gcgcgcccag gagtgggcgt cggtccagct gggctcgatc tacaacgcgc tcaagacggg
      421 gtgagtcagg tgagacgtgc ggtcgagcgt caccgcgagc gcgcctgccg aggaccccct
      481 cgccgcgcgc agttccgcag cgctccccac gagcgaaccg agaaaccgcg aggcgagcgc
      541 gagcacatcg cgcacgatct cgccaagcgc gaggactgtt tgagcacggc gaggggaggc
      601 cgacggcgag cgcgagcggc gcctgtgttc ttcgccggtg tctgtgcgag cggcgccgcc
      661 gcgctcgccg ccggcctcgc cgtcgcgacc cgcacgtttc gccgcgaatc gcgtgacgcg
      721 cgatcgctac gacttgttcc gcgggcggaa tatcgctcag cgcagctcga actccttgca
      781 gaacgcggcc tcggtcggcg gcgagccgtc ggggttcggg gcatcgagcg gccaccgccg
      841 ctggccctcg tccggccagt cgtgggcgca ggcgccgcgc tccgggcgcc agtagaagca
      901 gtcgcggcac gccgagcgca gggcgaatcg cctcgcctcg tcgagcagct gctcggtgag
      961 ccggaacttc actgcgaggc ttcggcgccc gagccgtgcg cggccgcctc gcgccccgag
     1021 ccggtcgtgg cgtggatgcc gcgctccagg cgctcgaccg ccgggcgcgc cctctccttc
     1081 acaccgtcct tgagatctcg gacttcttcg gtgtgccaga tcgcccggat atgaccgacc
     1141 agggtgtgct tgccgagctg cacggtggtc gcgaaccacg ccagggcgat cacgattgcg
     1201 aggtacacga ggaatcggat catgattcaa ccgtagcgcg ggcgtgggcg cgcgccagtt
     1261 tcattcggta gcgcgttggt ttcagtcgtt cgtgcgcttg atgatcagga atgcgacgcg
     1321 ccggttcttg gcccaggccg cctcgttgtg gcggttgtcg agcggcatgg tctcgccata
     1381 tccgtgtgcg gtcaggcgtt tgggatcgac gcccttgtcg accaggtact tcatgaccgc
     1441 cttggcgcgg cgatcggaca gttcgaggtt gtaggtgtcg tcgccgcgct cgtcggtgtg
     1501 gccctggatc tcgaccagct ggatgctcgg gttgccctgc agggtcgcgg cgaccgcgtc
     1561 gaggatcggg aacgacttgg gcaggatcac cgccttgtcg tactcgaagt agatcacgtc
     1621 gaggatctcg atcgaggtgt cggtgaccac gacccggccg cggtcgggac agccgtcctc
     1681 gtcctggtag ccgttgtagg tttcgggctc gttggggcac ttgtcgtcct tgtccgggat
     1741 ccgatcgtgg tcgttgtcgg gatccgggca gccgtcggcg tcctcgaagc cgtccttgtc
     1801 ctcggggtcg ttggggcaca ggtcgacgtt gtcggggatg ccgtcgccgt cgttgtcggg
     1861 atcggggcag ccgtcctcgt cctggaaccc gtccttgtcc tcgggctgat ccgggcactt
     1921 gtcgacgttg tcggggatgc cgtcgccgtc gcggtcgttg ttggcggtga tcgggcagcc
     1981 gtcgttctcc ttggggccgg ggatgtcggg gcagcggtcg tcgacgtcgg gcacgccgtc
     2041 gtggtcgttg tcggggtcgg ggcagccgtc ctcgtcctcg aagccgtcga agtcctcggg
     2101 ctcgtcgggg cacttgtcga cgtcgtcctt gatgccgtcg ccgtcgcggt ccccgatgtt
     2161 gggctcgaac acgatgccga tcatcgcgcg gaccccgggg ttggcgccct tgtcggggac
     2221 caggccgcgg ccgacgccga gcgacaggaa cgagttgcgc gcgaggtaca gcttgacgcc
     2281 gccgagcacc tcgagcggct ggtagttggt gtgatcgccg agcgggatcg agccgatcac
     2341 ctcgccgacc agatcgaact tctggcgcga cagcgcgtag gcgatgccga acccgaacgg
     2401 gatctcggag cctgcggtga ccgtgccgtt ggtgaccggg gcgttgtcga tgccgggatc
     2461 gctgttggtg aacgtctcgg cgctgcggat ccggatcccg gcgttgagcg acatccgcag
     2521 cgcgccggtc cggccgaact ccttgtcgag gatgcccatc acctggggca cgagcttgcc
     2581 gccggtcgtg ccgttcgagc ggctggcctc gccgaggaac cgatcggtcg ggttggtggt
     2641 cggcaggtac aggctcgcga tcacgccgag gccgatgtgc ggcccgcggc tggtcttgag
     2701 gaaccgggtc ttgaagtgga cgccgatgtt gccgatgccc tggccgtcga gcttgaactc
     2761 gttctgaccg acgtcggggc cgcgatcgcc gctcatgatg aggaacggca ccgacgcacc
     2821 gaactcgagc tcggccggtc cggcgtggat gccgaacgcg ccgatcagcg tcgcgccgat
     2881 catgtcgtcg atcgagtagg tgtggcccat gccgtcgagc ttgagcaggc tgtggcccca
     2941 gtcgagcgcg ccgagcccga acgagatctc cttgtcgccc aggacctgcg aggcattgac
     3001 cgtgaggtag ccgcgcgagt ccatcgccgg ccggaacgca ttgagatcga tgttgccggc
     3061 gggctccgcg accgcgagcg cggacagcca gacagcgccc aggcccccca ggacggcggc
     3121 atcgacgca
//
LOCUS       DASVOR010000725         9437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_182590,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000725 DASVOR010000000
VERSION     DASVOR010000725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9437
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182590"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..263)
                     /locus_tag="VF516_14280"
     CDS             complement(<1..263)
                     /locus_tag="VF516_14280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108894.1"
                     /translation="MKTLTEFSTLTLRKAAAARAAAGGAKVGAPAAELDDAMDASERA
                     AADAPPEDAAAEASGEAEAEASGEAETEGQTSGEPPAGDAQGEA"
     gene            complement(332..1381)
                     /locus_tag="VF516_14285"
     CDS             complement(332..1381)
                     /locus_tag="VF516_14285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108895.1"
                     /translation="MRNPFPLIAAALALAVAALGGCPKNVPGAGSVPSVPGGGGVPGG
                     LGGVSGEVDPNTCGNYALMDGGAKLKAFLQATKDLEITSEETVKVVKQSCEMIGREMG
                     MTDADFAGDTKAVCDKVYKTLRDNMKVAFKAQAGLKIKYKPAVCRVNVEAEAKAAAEC
                     EGHASADVGASCTGACHGRCDGTCSGKAGTGGNSAQCDGECKGTCHGECEGHAKVNAS
                     AQCRASASVKANAEVHCSEPELTVEAAAKAELDKSKAEMVIRALRNGLPKALSIQAHL
                     EPLKDAVAIWAQSAQELKDMGPKFINSFKDQAMCISGQLAAAVALIGKINTNVSVSVD
                     VSVQASATASGSAGA"
     gene            complement(1603..3207)
                     /locus_tag="VF516_14290"
     CDS             complement(1603..3207)
                     /locus_tag="VF516_14290"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEX8108896.1"
                     /translation="MPPERTTLGAIDAGSNAIRMVVAELGPSELVRIEAERVPVRLGH
                     GAFTRGELDDAVIDQAVAAFVHFRERFDHHGVTIYRAVGTSAVRNATNRDVLLHRLYH
                     EAGIELEVIDGEEEARLVRKSVVHSLGPTGTPPRAILDLGGGSLEVNLRYGPTWRGYS
                     LPVGTVRLMETFGLDGAIAEAEAGMVRRYTATLMHTIARGATGNGLAAATGGNAEALA
                     KIVGDGNPVMPSFDLAALEKALPSIVGVSVEERMAQFGVKRDRAEVLAIAALVFATAA
                     RQLGISRLVAPGVGVREAVLLELAETAREEQAKAEGAHDKALLTAARAFANRVDHDTT
                     HGEHVRQLARTLFHQLRDVHGLPGELGVVLEVAALLHDVGEVVNVRGHHKHSEYMIRW
                     ARIPGLDDTSREMIALLARTHRKDAARAKQIINESSLAKDRRAQVRRLSGLLRVADAL
                     DSEHRSRVEQIVCTRMGDAVVLDLVVRDGPSRDDAQLLRKSDLLVEELGMPIKITVAR
                     PISSPGTPPVEAAGGGTVVAIRALHR"
     gene            complement(3314..4474)
                     /locus_tag="VF516_14295"
     CDS             complement(3314..4474)
                     /locus_tag="VF516_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8108897.1"
                     /translation="MKKVISIVFGLAVLVIIGGAFVYLWKKSQAKPVVWQTEHAEVTD
                     ITKKTVATGSIVPRQEVDVKPKVTGVLSELYVAPGKIVKLGDPLGKISIIPDAMAMNQ
                     AESQVRTAQIGFDNAKRELERNEALFKQGVVADAELQKFRTDFAMARQQLDVAGSNLQ
                     LVREGATRAQGKSSTLIVTATTAGMVIDVPVKVGFSVIQANNFNPGTTVAVIADMDDM
                     IFDGRVDEAEVAKVKEGMTLKIKVGAIEKDTLEGKLEYIAPKGKEIDGAIQFEIKAAV
                     KQKPGIQIRANYSANADIVLEEKKQVLAVREALVQYDHDKPYVEIETTPQTFVRRDVK
                     LGLSDGIKVEVLSGVTPADRVKIPDNAGPAVPAAGSGSGAGSGSAAKPPPRK"
     gene            complement(4480..5733)
                     /locus_tag="VF516_14300"
     CDS             complement(4480..5733)
                     /locus_tag="VF516_14300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8108898.1"
                     /translation="MFDLDNWQEVWATLRSNLLRTFLTALGVCWGIFMLMAMLGFASS
                     MQEGTKRQMKGIATNVLWVWGQTTTVAYDGMPIGRQVKFDISDIENLRRLPDIEWLAP
                     RLRLGGWMSGYNLSYEGKTGSYAVFADYPDFRHIIAFEYEAGRFINDRDIAEQRKVVV
                     IGRAIRDELFGAGVDPIGKYVKISGVYFEVVGITRTLRSGQAGDQDAHTAFVPFTTLK
                     SAFHMGDRVGFFALTARPGSDGPELERQVRATLAKRHRLSPDDQLAIGSFNAFVFFGK
                     IQMFFIVLSIVGWVFGGATLLAGVVGVSNIMLITVKERTKEIGVRKALGATPMSIVAM
                     VMKESIVLTLIAGLVGVAAGTLLLAVGGSVFQSMPDSPMGPPLLAFATVMAAVAVLIA
                     SGAAAGIMPAAHAASIRPIEALRTE"
     gene            complement(5745..7121)
                     /locus_tag="VF516_14305"
     CDS             complement(5745..7121)
                     /locus_tag="VF516_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8108899.1"
                     /translation="MIVALLLVGGVLALGIAALLAITLLRISGVLLVQWRDLLSIDRW
                     AEVFDTIRRNKLRTALTMIAVSWGIFVLVFLLGLGKGLDNGLRHTFARDATNGIWMAA
                     AKTSIAHDGYDVGRKLTFDNRDYARVQSAKVPGPAGPGIDHISGQFFIRGGQFGGGEM
                     VIKYNGKANPFQINAVHPDAIYLAAQMVESGRFLDFVDIEHRRKSAVIGRGVRDFLFK
                     DENPLGKWITVVGVPFQVVGVFSGEGNAEQERQIYIPVTTAQLAFNGADRLGMLEFTI
                     GDANAEEAKAIADQVVAQLAERHHFDPKDPQAVRVHNNVEQFQRFSKMFLAISIFVVF
                     IGVLTLLAGVIGVSNIMMIAVKERTKEIGVRKALGATPNSIIAMIIQEAIFLTGVAGL
                     LGLSAGVALLELVSRAVQNEFIRNPAIDLRIGVAATIGLVIAGALAGFFPARTAARVN
                     PVHTLRDQ"
     gene            complement(7118..7858)
                     /locus_tag="VF516_14310"
     CDS             complement(7118..7858)
                     /locus_tag="VF516_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8108900.1"
                     /translation="MLRLEKVNKYYRVATHSLHALRDVDFEVQDGECVAIMGASGSGK
                     STLLNILGILDDYDTGEYWLGDTLIRDLSEKKAANYRNRFIGFVFQSFNLLPFKTALE
                     NVALPLYYQGITRRKRNQIATQYLERVGLRDWSEHTPAEMSGGQKQRVAIARALIGKP
                     RLILADEPTGALDSETSSQIMDLLTEINRTGITVLVVTHDQEVASRTQRTIRLRDGQI
                     VDSVISGTNTPHTPTTTAAGRSAAMTPR"
     gene            complement(7942..8451)
                     /locus_tag="VF516_14315"
     CDS             complement(7942..8451)
                     /locus_tag="VF516_14315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX8108901.1"
                     /translation="MAKLPTRSPTHEDVPAGRLAAMGLELEDIADGDTVALPALIELA
                     EDRGRPASHYLAAVALATELRVAPAAPVTVRVCAGNCQRWGALDVLDHLAGQLSGQRS
                     GQRSGQLAGQLSGRHAGGAPRFAIAPVSCLDRCDHAPACEIHGTHGQLVVAPATPAAI
                     DDALRELAG"
     gene            complement(8461..8823)
                     /locus_tag="VF516_14320"
     CDS             complement(8461..8823)
                     /locus_tag="VF516_14320"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX8108902.1"
                     /translation="MNSSLAISLGVALCAAGCGGTGLGADTRADITARMTSAQQPLAA
                     CYHAGLKTNRKLAGTVVVAFAAAPSTGQFTDIQVTRDELGDPGVRQCVIDEVGKLKLA
                     KPQATRLSISYPIHFAPN"
     gene            complement(8816..9334)
                     /locus_tag="VF516_14325"
     CDS             complement(8816..9334)
                     /locus_tag="VF516_14325"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8108903.1"
                     /translation="MRHLLFAALVAAGPGCSLITVQQDPFPPLEVRADRPPATPPRVV
                     LTASSITIGEKVQFATGSAEILAVSHGLLDEVAKVLTDNLQIELLQVEGHTDSTGTAA
                     LNRKLSQQRAESVMKYLASKGIAAGRMQAKGFGPDKPIADNATDDGREKNRRVEFNIL
                     KQGPKKTVVRDE"
BASE COUNT         1341 a         3243 c         3327 g         1526 t
ORIGIN      
        1 gcctcgccct gcgcgtcgcc ggccggtggt tcgcccgagg tctggccctc ggtctcggcc
       61 tcgccggacg cctcggcctc ggcctccccg gacgcctccg cggcggcgtc ctccggcggt
      121 gcgtccgccg cggcgcgctc ggatgcatcc atcgcatcgt cgagctccgc ggccggtgcg
      181 cccacctttg cgcctcccgc ggcagcgcgt gcagccgctg ccttgcgcag ggtaagcgtc
      241 gagaattcgg tgagcgtctt catgggaggc tagctacacc aaagggtccc tgtgcgccag
      301 gggtgcgacg ggcgatcgca ggtccccgga ctcaggcacc ggccgagccg ctggcggtcg
      361 cgctggcctg gaccgacacg tcgaccgaga ccgacacgtt ggtgttgatc ttgccgatca
      421 gggcgacggc ggcggcgagc tggcccgaga tgcacatcgc ctgatccttg aatgaattga
      481 tgaacttggg ccccatgtcc ttgagctcct gggcgctctg ggcccagatc gccaccgcgt
      541 ccttgagcgg ctccaggtgg gcctggatcg acagcgcctt gggcaggccg ttcctgagcg
      601 cccggatcac catctcggcc ttggacttgt cgagctccgc cttggcggct gcctcgaccg
      661 tgagctcggg ctcggagcag tggacctcgg cgttggcctt gaccgacgcg ctggcccggc
      721 actgggccga ggcgttgacc ttggcgtggc cctcgcactc accgtggcag gtgcccttgc
      781 actcgccgtc gcactgcgcg ctgttgccgc cggtgccggc cttgcccgag caggtgccgt
      841 cgcagcggcc gtggcaggcg ccggtgcagc tcgcgccgac gtcggccgac gcgtggccct
      901 cgcactcggc ggcggccttg gcctcggcct cgacgttgac ccggcagacc gcgggcttgt
      961 acttgatctt gagcccggcc tgcgccttga acgcgacctt catgttgtcg cgcagcgtct
     1021 tgtagacctt gtcgcagacc gccttggtgt cgccggcgaa gtcggcgtcg gtcatcccca
     1081 tctcacggcc gatcatctcg cagctctgct tgacgacctt gaccgtctcc tcgctggtga
     1141 tctcgaggtc cttggtcgcc tggaggaacg ccttgagctt ggcgccgccg tccatcagcg
     1201 cgtagttgcc gcaggtgttc ggatccacct cgccggacac gccgccgagg ccaccgggga
     1261 ccccaccgcc gccgggcacg ctggggaccg agccagcgcc gggcacgttc ttgggacaac
     1321 caccgagcgc cgcgaccgcc agggcgagcg cagctgcgat caaagggaag ggattgcgca
     1381 tgtcgggatg gttatcaaaa actcgcaggg aacgcggggt tcgggcggcc aaaaacgcgc
     1441 gcccgggccc gcggtggggg cctgcatcag gccggcctcg cgcacccgtg gcggaaaact
     1501 gccgccatgc gcggaacgcc ccgccccgag atggcaacga tggcaccgag acggtgcccg
     1561 aggcgatgcc ggggcgatgc cgggacgatg ccgggacgaa gatcaacggt gcagcgcgcg
     1621 gatcgccacc accgtgccac cgccggcggc ctcgaccggc ggggtgccgg gggatgagat
     1681 aggccgcgcg accgtgatct tgatcggcat gccgagctcc tcgaccagca ggtcggactt
     1741 gcgcagcagc tgggcgtcgt cgcgcgacgg cccgtcgcgg accacgaggt cgagcacgac
     1801 cgcgtcgccc atccgcgtgc acacgatctg ctcgacccgc gagcggtgct cgctgtcgag
     1861 cgcgtcggcg acgcgcaaca ggccgctgag ccggcggacc tgggcccgcc gatccttggc
     1921 caggctcgac tcgttgatga tctgcttggc gcgcgcggcg tccttgcggt gggtccgggc
     1981 gagcagcgcg atcatctcgc gcgaggtgtc gtcgagcccg gggatccgcg cccaccggat
     2041 catgtactcg ctgtgcttgt ggtggccgcg cacgttgacc acctcgccga cgtcgtggag
     2101 cagcgccgcg acctcgagca cgacgccgag ctcgccgggc aggccgtgga cgtcgcgcag
     2161 ctggtggaac agcgtgcgcg cgagctggcg gacgtgctcg ccgtgcgtgg tgtcgtggtc
     2221 gacccggttg gcgaacgcgc gggccgcggt gagcagcgcc ttgtcgtgcg cgccctcggc
     2281 cttggcctgc tcctcgcgcg cggtctcggc gagctcgagc agcaccgcct cgcggacgcc
     2341 gaccccgggc gcgaccagcc gcgagatccc gagctggcgc gcggcggtgg cgaacaccag
     2401 cgccgcgatg gcgagcacct cggcgcggtc gcgcttgacc ccgaactgcg ccatgcgctc
     2461 ctcgaccgac accccgacga tcgacggcag ggccttctcc agcgcggcga gatcgaagct
     2521 cggcatcacc gggttgccgt cgccgacgat cttggccagc gcctcggcgt tgccgccggt
     2581 cgccgccgcc agcccgttgc cggtcgcacc gcgcgcgatc gtgtgcatca gcgtggccgt
     2641 gtagcgccgc accatgccgg cctcggcctc ggcgatcgcg ccgtcgagcc cgaacgtctc
     2701 catcaggcgc acggtgccga ccggcagcga gtagccgcgc caggtgggcc cgtagcgcag
     2761 gttgacctcc aggctgccgc cgccgaggtc gaggatcgcg cgcggcggcg tcccggtcgg
     2821 gccgagcgag tggaccaccg acttgcggac cagccgggcc tcctcctcgc cgtcgatgac
     2881 ctcgagctcg atcccggcct cgtggtagag ccggtggagc aggacgtcgc ggttggtcgc
     2941 gttgcgcacc gcgctggtgc cgaccgcgcg atagatcgtc acgccgtggt ggtcgaatcg
     3001 ctcgcggaag tggacgaacg ccgcgaccgc ctgatcgatc accgcgtcgt cgagctcgcc
     3061 gcgggtgaac gcgccgtggc cgagccggac cggcacgcgc tcggcctcga tccggaccag
     3121 ctcgctcggc cccagctcgg cgacgaccat ccggatcgcg ttcgatccgg cgtcgatcgc
     3181 gcccagcgtg gtccgctcgg gaggcatcac gagcaagcta gcacgctggc tgcccggtca
     3241 cggtgccggt caccccggag cgtgcccgga gagcgcgatc cgatcccggg tgagcggagc
     3301 cgctcgctgc gcgctacttc ctgggcggcg gcttggcggc gctccccgat ccggcgccgg
     3361 agcccgaccc ggccgccggc accgccgggc ccgcgttgtc ggggatcttc acgcggtcgg
     3421 ccggggtcac acccgacagc acctcgacct tgatgccgtc ggacagcccg agcttgacgt
     3481 cgcgccggac gaaggtctgg ggcgtcgtct cgatctcgac gtagggcttg tcgtgatcgt
     3541 actggaccag cgcctcgcgc accgcgagca cctgcttctt ctcctcgagc acgatgtcgg
     3601 cgttggcgct gtagttggcc cggatctgga tgccgggctt ctgcttgacc gccgccttga
     3661 tctcgaactg gatcgcgccg tcgatctcct tgcccttggg cgcgatgtac tcgagcttgc
     3721 cctcgagcgt gtccttctcg atcgcgccga ccttgatctt gagggtcatg ccctccttga
     3781 ccttggcgac ctcggcctcg tcgacccggc cgtcgaagat catgtcgtcc atgtcggcga
     3841 tcaccgcgac cgtggtcccg gggttgaagt tgttggcctg gatcaccgag aacccgacct
     3901 tgaccggcac gtcgatcacc atgcccgcgg tggtcgcggt cacgatcagg gtcgacgact
     3961 tgccctgggc gcgggtcgcg ccctcgcgga ccagctggag gttcgagccc gcgacgtcga
     4021 gctgctggcg cgccatcgcg aagtcggtgc ggaacttctg gagctcggcg tcggcgacca
     4081 cgccctgctt gaacagcgcc tcgttgcgct cgagctcgcg cttggcgttg tcgaacccga
     4141 tctgggcggt gcggacctgc gactcggcct ggttcatcgc catcgcgtcg gggatgatcg
     4201 agatcttgcc cagcggatcg ccgagcttca cgatcttgcc cggagcgacg tagagctcgc
     4261 tgagcacgcc cgtgaccttg ggcttcacat cgacctcctg gcgcggcacg atcgagccgg
     4321 tcgccacggt cttcttggtg atgtcggtca cctcggcgtg ctcggtctgc cacaccaccg
     4381 gcttggcctg cgacttcttc cacaggtaga cgaacgcgcc gccgatgatc acgaggacgg
     4441 ccaggccgaa gacgatggag atgactttct tcatggagct cactcggtac ggagcgcctc
     4501 gatcgggcgg atcgaggcgg catgagcggc cggcatgatg ccggcggccg cgccggatgc
     4561 gatcaacacg gcgaccgcgg ccatcacggt ggcgaaggcg agcaggggcg ggcccatcgg
     4621 tgaatcgggc atgctctgga acacgctgcc gccgaccgcg agcaagaggg tgccggcggc
     4681 gacgccgacg aggcccgcga tcaacgtgag cacgatcgac tccttcatca ccatcgcgac
     4741 gatcgacatc ggggtcgcgc cgagcgcctt gcgcacgccg atctccttgg tgcgctcctt
     4801 gacggtgatc agcatgatgt tcgagacccc gaccacgccg gcgagcaagg tcgcgccgcc
     4861 gaacacccag ccgacgatcg acagcacgat gaagaacatc tggatcttgc cgaagaacac
     4921 gaacgcgttg aacgagccga tcgcgagctg gtcgtcgggc gacagccggt gccgcttggc
     4981 gagcgtggcg cggacctggc gctcgagctc ggggccgtcg ctgccgggcc tggcggtgag
     5041 cgcgaagaac ccgacgcgat cgcccatgtg aaatgcgctc ttgagcgtcg tgaacggcac
     5101 gaacgcggtg tgcgcgtcct ggtcgcccgc ctgtcccgag cgcaaggtcc gggtgatgcc
     5161 gacgacctcg aagtacacgc cgctgatctt cacgtacttg ccgatcggat cgacgccggc
     5221 gccgaacagc tcgtcgcgga tcgcccggcc gatcaccacc accttgcgct gctcggcgat
     5281 gtcgcggtcg ttgatgaacc ggccggcctc gtactcgaac gcgatgatgt ggcggaagtc
     5341 ggggtagtcg gcgaacaccg cgtagctgcc ggtcttgccc tcgtagctga ggttgtagcc
     5401 gctcatccag ccgccgagcc ggagcctggg cgcgagccac tcgatgtcgg gcaggcgacg
     5461 caggttctcg atgtcggaga tgtcgaactt cacctgccgc ccgatcggca tgccgtcgta
     5521 ggcgaccgtc gtggtctgcc cccacaccca cagcacgttg gtcgcgatcc ccttcatctg
     5581 acgcttcgtg ccctcctgca tgctcgacgc gaagccgagc atcgccatca gcatgaagat
     5641 cccccagcac acaccgagcg cggtcaggaa cgtgcgcagc aggttgctgc gcagcgtcgc
     5701 ccagacctct tgccagttgt cgagatcgaa catgatgggt ctcgttattg gtctcgcaag
     5761 gtgtggacgg ggttgacgcg ggcggcggtg cgcgcgggga agaagccggc gagcgcaccg
     5821 gcgatcacca ggccgatcgt cgcggcgacg ccgatgcgca ggtcgatcgc aggattgcgg
     5881 atgaactcgt tctggaccgc gcggctgacg agctcgagca gcgcgacgcc ggccgacagc
     5941 ccgagcaggc ccgcgacccc ggtcaagaag atcgcctcct ggatgatcat cgcgatgatc
     6001 gagttgggcg tggcgccgag cgccttgcgg acaccgatct ccttggtgcg ctccttgacc
     6061 gcgatcatca tgatgttcga gacgccgatc accccggcga gcagcgtgag cacgccgatg
     6121 aagacgacga agatcgagat cgccaggaac atcttgctga accgctggaa ctgctcgacg
     6181 ttgttgtgga cgcggaccgc ctgcgggtcc ttgggatcga agtggtggcg ctcggcgagc
     6241 tgcgcgacca cctggtcggc gatggccttg gcctcctcgg cgttcgcgtc gccgatcgtg
     6301 aactcgagca tgccgagccg gtcggcgccg ttgaacgcca gctgcgcggt cgtcaccggg
     6361 atgtagatct ggcgctcctg ctcggcgttg ccctcgccgc tgaacacgcc gaccacctgg
     6421 aacggcacgc cgacgacggt gatccacttc ccgagcgggt tctcgtcctt gaacaggaag
     6481 tcgcgaacgc cgcgcccgat caccgccgac ttgcggcgat gctcgatgtc gacgaagtcg
     6541 aggaacctcc cggactcgac catctgcgcc gcgaggtaga tcgcgtcggg gtgcacggcg
     6601 ttgatctgga acgggttggc cttgccgttg tacttgatca ccatctcgcc gccgccgaac
     6661 tggccgccgc ggatgaagaa ctgacccgag atgtggtcga tgcccgggcc ggcggggccg
     6721 gggaccttcg ccgactggac gcgcgcgtaa tcgcggttgt cgaacgtcag cttgcggccg
     6781 acgtcgtagc cgtcgtgggc gatgctggtc ttggccgccg ccatccagat gccgttcgtc
     6841 gcatcgcgcg cgaaggtgtg gcgcaggccg ttgtcgaggc ccttgccgag cccgagcagg
     6901 aacaccagca cgaagatgcc ccacgacacg gcgatcatcg tcagcgccgt gcgcagcttg
     6961 ttgcggcgga tcgtgtcgaa cacctcggcc cagcggtcga tcgacagcag atctcgccac
     7021 tggaccagca gcacgcccga gatccggagc agggtgatcg cgagcagcgc tgcgatcccg
     7081 agcgcgagga cgccgccgac gaggaggagc gcgactatca tcgtggagtc atcgccgccg
     7141 acctgccggc cgctgtggtc gtgggagtgt gcggggtgtt ggtcccggag atcaccgagt
     7201 cgacgatctg gccgtcgcgc agccggatcg tgcgctgtgt gcggctggcg acctcctggt
     7261 cgtgggtcac gaccaggacc gtgatcccgg tccggttgat ctcggtcagc aggtccatga
     7321 tctggctcga ggtctcgctg tcgagcgcgc cggtcggctc gtcggcgagg atcagccgcg
     7381 gcttgccgat cagcgcgcgc gcgatcgcga cccgctgctt ctggccgccg gacatctccg
     7441 ccggggtgtg ctcggaccag tcgcgcaggc cgacccgctc gaggtactgc gtcgcgatct
     7501 ggttgcgctt gcgccgcgtg atgccctggt agtacagcgg caaggcgacg ttctcgagcg
     7561 cggtcttgaa cggcagcagg ttgaacgact ggaacacgaa gccgatgaac cggttgcggt
     7621 agttcgcggc cttcttctcg ctgaggtcgc ggatcagggt gtcgcccagc cagtattcgc
     7681 cggtgtcgta gtcatcgagg atgcccagga tgttgagcaa ggtcgacttg cccgagcccg
     7741 acgcacccat gatcgccacg cactcaccgt cctggacctc gaagtcgacg tcgcgcagcg
     7801 cgtgcaggct gtgggtcgcg acccggtaat acttgttgac cttctcgagc cgcagcaccc
     7861 gaaccaggct agcgctaacc gtgaacgcga gccagcccaa gacgcgtgcg acacgtgtga
     7921 cagcgcaggt cgcagctgtc gctagccggc gagctcgcgg agcgcgtcgt cgatcgcggc
     7981 cggcgtggcc ggtgcgacga cgagctggcc gtgcgtgcca tggatctcgc acgccggcgc
     8041 gtgatcgcag cggtcgaggc agctgaccgg cgcgatcgcg aaccgcggcg cgccgccggc
     8101 gtgacgtccg gatagctgcc cggcgagctg tccggatcgc tgtccggatc gctgtccgga
     8161 tagctgcccg gcgagatgat cgagcacgtc gagcgcgccc cagcgctggc agttcccggc
     8221 gcacacccgg acggtgaccg gcgcggccgg cgcgacccgg agctcggtgg cgagcgccac
     8281 cgccgcgagg tagtggctgg ccggccggcc gcggtcctcg gccagctcga tcagcgcggg
     8341 cagggcgacg gtgtcgccat cggcgatgtc ctcgagctcc agccccatcg ccgccaaccg
     8401 gccggccggg acgtcctcgt gggtcgggga tcgggtcggg agcttggcca tggtcacccc
     8461 ctagttcggg gcgaagtgga tcgggtagct gatcgacagc cgggtcgcct gcggcttggc
     8521 gagcttgagc ttgccgacct cgtcgatcac gcactggcgg acgccgggat cgccgagctc
     8581 gtcgcgcgtg acctggatgt cggtgaactg gccggtgctg ggcgcggcgg cgaacgcgac
     8641 gaccacggtg ccggcgagct tgcggttggt cttcagcccg gcgtggtagc acgcggcgag
     8701 cggctgctgc gccgaggtca tccgcgcggt gatgtcggcg cgggtgtcgg cgcccaggcc
     8761 ggtgccgccg cagccggcgg cgcacagcgc gacgccgagc gagatcgcga gggagctatt
     8821 catcgcgcac caccgtcttc ttggggccct gcttgaggat gttgaactcg acgcgccggt
     8881 tcttctcccg gccgtcgtcg gtcgcgttgt cggcgatcgg cttgtcgggc ccgaacccct
     8941 tggcctgcat ccggccggcg gcgatgccct tggacgcgag gtacttcatc accgactcgg
     9001 cgcgctgctg cgacagcttg cggttgagcg ccgcggtgcc ggtcgagtcg gtgtggccct
     9061 cgacctggag cagctcgatc tgcaggttgt cggtcaggac cttggcgacc tcgtcgagca
     9121 ggccgtgcga gaccgccagg atctcggccg agccggtggc gaactggacc ttctcgccga
     9181 tggtgatgct cgacgcggtc agcaccacgc gcggcggcgt ggccggtggc cggtcggccc
     9241 ggacctcgag cggtgggaat gggtcctgct ggaccgtgat caacgaacaa cccggcccgg
     9301 cggcgacgag cgccgcgaag aggaggtgtc gcatggccag gcatgataca gcgatcgggc
     9361 catgatcacg gtccaccacc tcaataactc gcggtcgcag cgcgtgctgt ggctgctcga
     9421 ggagctcgcg ctgccct
//
LOCUS       DASVOR010000726         6105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415534,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000726 DASVOR010000000
VERSION     DASVOR010000726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6105
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415534"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1231
                     /locus_tag="VF516_14330"
     CDS             <1..1231
                     /locus_tag="VF516_14330"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8108904.1"
                     /translation="PVDAAFDPLSPAYLDDPYAILAALPLAEAPIFYAPSIGYYVVTR
                     YADIAQVFDDPATYSAAVAQAPLVPLSPEAQRILLDGGHQPQPSMVSLDEPAHARLRK
                     PAARAFAVRRVNDLVPTIRRVAAELLDAVAAGGAEFDLIDALAFPLPASIVFSLIGVP
                     EADVPQLKVWCGHRAALSWGRPRPEDQVEIATHMAAYRRYLRELVDRKRAARGDDLTG
                     DLLAIHDEDPERLTLAEIASILFSLSFAGHETTTGLIGNTVRRLLEAPERWDAIIRDP
                     ALIPAAVDESLRFDPSVPVWRRVTTRPVALAGALLPRGARLFLWLAAANRDASVFPDP
                     ERFDLHRHNADQHLAFGKGLHYCLGAHLGKVEARIVLDELTRRFPRLRLVPGQRLSFH
                     PNISFRGPQQLRVRAAP"
     gene            complement(1280..1585)
                     /locus_tag="VF516_14335"
     CDS             complement(1280..1585)
                     /locus_tag="VF516_14335"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8108905.1"
                     /translation="MKTKTGCAVPWHWHTANEQLMIVDGTARVEMKDMGKPITLQAGG
                     FAMPSHHVHEFRCTQACTFLLYSDQKLDIHYVDPQGKEISPDEALKPVRETAATAMK"
     gene            2137..3105
                     /locus_tag="VF516_14340"
     CDS             2137..3105
                     /locus_tag="VF516_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HEX8108906.1"
                     /translation="MRLALGPGVLGLLAVVQAACFNPHVPRGVPCSVVDRSCPEGQAC
                     VAGFCGGTLGMDAAVDATPDAFIDSDGDKVPDAIDNCPHVPNPDQGDEDKDGLGDLCD
                     PCPIDADNTDPDGDGVAGLCDPHPNTPGDKIVAFQGFHGAIPSDEWKVVGAGTIATDK
                     DSAVITNTANQVTALVALGAGTTFGNGMIMASVIVDATPGGANVRTALTVGLPYNSAT
                     GVGILCQLHAPNPDSTNGRELSLFDSKVNQEQAFNPFVWDTGAAYRLTMIRTGGSNYA
                     CSETDAGGTVKTATNSTGSIPAQSEVALVASGTSAHVAWVLVVSSP"
     gene            complement(3133..3657)
                     /locus_tag="VF516_14345"
     CDS             complement(3133..3657)
                     /locus_tag="VF516_14345"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8108907.1"
                     /translation="MEIRSLRASDDRSVFHSGDEALDRFFHRYAGQNQFRHHLGVTYV
                     AVDGDRILGFATVAPRHIDIEDLPERLRKKLPRYPIPVLGLARLAVDTSARSIGLGSQ
                     LLRFVLQLAAKLADEVGCAGVVVDAKPGAVELYARYGFTAFDALEGQSDARPQTTLTW
                     LPIQAVEHATKPPH"
     gene            complement(3647..3916)
                     /locus_tag="VF516_14350"
     CDS             complement(3647..3916)
                     /locus_tag="VF516_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108908.1"
                     /translation="MVKETQISAQISETTRGLMEKHVRRTGVKKGHLIEQALLHHLQA
                     LDELPAEYVVHPRIVVSRKTGEDLLRRAEAAEPTPALRKLMRDGD"
     gene            4074..4817
                     /gene="pyrH"
                     /locus_tag="VF516_14355"
     CDS             4074..4817
                     /gene="pyrH"
                     /locus_tag="VF516_14355"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEX8108909.1"
                     /translation="MVKPAYKRILLKISGEALMGHQGYGINPEVLTHVAGEIAETARL
                     GVEVAVVVGGGNIFRGVSESSRGMDRASADYVGMLATVMNSVSLQEAIEKQGVVTRVQ
                     SAIPMSQLAEPYIRRRAIRHLEKGRVVIFGAGTGNPFFTTDTAAALRAMEIGAEILLK
                     ATKVDGVYDKDPAKHSDARRYATVTFTDALKQDLKVMDATAFALCRDNEMRIIVFDMT
                     TRGNIHRVVCGEPVGTTIVKDNQQTRFAN"
     gene            4836..5393
                     /gene="frr"
                     /locus_tag="VF516_14360"
     CDS             4836..5393
                     /gene="frr"
                     /locus_tag="VF516_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEX8108910.1"
                     /translation="MINDITSDTEDGMKKAVDSFKRDLQKIRTGRANTAMLDGIKVDY
                     YGTPTPVNQVATVQVVDARLITIKPWEKSMLQVIDKAIRASDLGINPVADSELVRLPI
                     PPLTQERRKELAKVVGKQTEEARVAVRSARRDAMDMVKDAEKDKQITEDERKNGEKKI
                     QELTDKYIAMVDDIAKAKEKEIMEL"
     gene            5425..>6105
                     /locus_tag="VF516_14365"
     CDS             5425..>6105
                     /locus_tag="VF516_14365"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108911.1"
                     /translation="MSGIPAGAGQLLGKRLGKYEILALLALGGTAEIYLARIAGAAGF
                     EKYVVVKCLHDHLADDTEFVKMFLDEARLAAHLDHSNIVQTMELGEHEGRYYMVMEFL
                     AGLSLAMIVRRVGERLPSMRVPVPLVLNILAQASAGLHYAHEKAMNGKALNIVHRDIS
                     PQNLVISFEGVVKLVDFGIAKAELRETRTRSGTIKGKFAYMSPEQCVANNVDRRTDVF
                     ALGVIGHEL"
BASE COUNT          982 a         2086 c         2149 g          888 t
ORIGIN      
        1 cccggtggac gcggcgttcg atccgctgtc cccggcgtac ctcgacgacc cgtacgcgat
       61 cctggccgcg ctgccgctgg ccgaggcgcc gatcttctac gcgccgtcga tcggctacta
      121 cgtcgtcacc cgatacgccg acatcgcgca ggtgttcgac gatccggcga cctactccgc
      181 ggcggtggcc caggcgccgc tcgtgccgct gtcgcccgag gcccagcgga tcctgctcga
      241 cggcggccac cagccgcagc cgtcgatggt gagcctcgac gagccggccc acgcccggct
      301 gcgcaagccg gcggcccgcg cgttcgccgt caggcgggtc aacgacctgg tgccgacgat
      361 ccgccgggtc gccgccgagc tgctcgacgc ggtggcggcg ggcggcgccg agttcgacct
      421 gatcgacgcg ctggcgttcc cgctgcccgc ctcgatcgtc ttctcgttga tcggcgtgcc
      481 cgaggccgac gtcccccagc tcaaggtctg gtgcggccac cgcgcggcgc tgagctgggg
      541 ccggccgcgc cccgaggacc aggtcgagat cgccacgcac atggcggcgt accggcgcta
      601 cctgcgcgag ctggtcgacc gcaagcgcgc ggcgcgcggc gacgacctga ccggcgacct
      661 gctggcgatc cacgacgagg accccgagcg gctcacgctc gccgagatcg cctcgatcct
      721 gttctcgctc agcttcgccg gccacgagac cacgaccggg ctgatcggca acaccgtgcg
      781 ccgcctgctc gaggcgcccg agcggtggga cgccatcatc cgcgatccgg cgctgatccc
      841 ggccgcggtc gacgagagcc tgcggttcga tccctcggtg ccggtgtggc gccgggtcac
      901 cacgcgcccg gtcgcgctgg cgggcgccct gctgccgcgg ggtgcgcggc tgttcctgtg
      961 gctcgccgcg gccaaccgcg acgcgtcggt gttccccgat cccgagcgct tcgacctgca
     1021 ccgccacaac gccgaccagc acctcgcgtt cggcaagggc ctgcactact gcctgggcgc
     1081 ccacctcggc aaggtcgagg cccggatcgt cctcgacgag ctcacccgcc ggtttccgcg
     1141 cctgcggctc gtccccggcc agcggctgag ctttcacccc aacatctcgt tccgcggccc
     1201 gcagcagctc cgcgtccgcg ccgcgccgta gcctgctgca cgacgcaggg cggctgccgc
     1261 cgggcacggg cgcacgccac tacttcatcg cggtggcggc cgtctcgcgc accggcttca
     1321 gggcttcgtc gggcgagatc tctttcccct ggggatccac gtagtggatg tcgagcttct
     1381 ggtcggagta gaggaggaac gtgcatgcct gggtgcatcg gaattcatgg acgtggtgcg
     1441 acggcatcgc gaagccgccg gcctggagcg tgatcggctt gcccatgtcc ttcatctcca
     1501 cgcgcgcggt gccgtcgacg atcatcagct gctcgttcgc cgtatgccag tgccacggaa
     1561 ccgcgcagcc ggtcttggtc ttcatcagcg cgacgaacgc accttgtgcc gggtcgccgc
     1621 tctggagacc tcgcggtcga cgcgtccagc tgcaggctgg gtgccggtag cccttcgcgg
     1681 ctgcacgaac gaacggtctt ctcagccccg gccgcgcagg atcacgatcg cggggtcatc
     1741 atgcacgggt gggatgcgcg ccagaagcgc gtcgtactgc tcgacggtga tcacgttcca
     1801 ttcgagcgct gctgcgaggt agccgcgcca ggcgatcgcc gttggctcgg ggcagctcgg
     1861 ggccggcggt cgcgctctgt gatggggatc gcacgggcga gggccccgga ccgtgcggac
     1921 cgcacgccac cagcaggacg aaccgcgcaa aggccgagcg caacaccgcg ccatcgtgcc
     1981 atgatgcgcg tcgcgggcat gtcgaacgga tggccatgga atctcacacg tccgccggga
     2041 agccccgacg ctgagcccgc gtatgcagcg atgatgcaga tcggcgcccc ggaataccgg
     2101 ccgaccgcgc cacgttggcg ggagaacgtg cgcgcgatgc ggttggccct cgggcccggt
     2161 gtgctgggcc tgctcgcggt ggtccaggcg gcgtgcttca acccgcacgt gccgcgcggg
     2221 gtgccgtgca gcgtggtgga caggtcgtgc cccgagggcc aggcgtgcgt cgccgggttc
     2281 tgcggtggga cgctgggcat ggacgcggcg gtcgatgcga cgcccgatgc gttcatcgac
     2341 agcgacggcg acaaggtccc cgatgcgatc gacaactgtc cccacgtgcc caaccccgac
     2401 cagggtgacg aggacaagga tgggctgggc gacctctgcg acccgtgccc gatcgacgcc
     2461 gacaacaccg accccgacgg cgacggcgtc gccggcctgt gcgatccgca ccccaacacc
     2521 ccgggcgaca agatcgtcgc gttccagggc ttccacggcg ccatcccgag cgacgagtgg
     2581 aaggtggtgg gcgccggtac catcgcgacg gacaaggact ccgcggtgat caccaacacc
     2641 gcgaaccagg tgaccgcgct ggtcgcgctg ggggcgggga cgacgttcgg caacggcatg
     2701 atcatggcga gcgtgatcgt cgacgcgacg cccggaggcg ccaacgtccg gaccgcgctg
     2761 accgtcgggc tgccctacaa ctcggcgact ggcgtgggaa tcctgtgtca gctccacgcg
     2821 cccaacccgg actcgacgaa cggccgcgag ctctcgctgt tcgactcgaa ggtgaaccag
     2881 gagcaggcct tcaacccgtt cgtgtgggac accggggcgg cgtaccggct cacgatgatc
     2941 cggaccggcg gcagcaacta cgcgtgctcg gagaccgacg caggtggcac tgtgaagacg
     3001 gcgaccaact cgacgggctc gatccccgct cagtccgagg tcgcgctggt cgcctccggc
     3061 accagcgcgc acgtcgcctg ggtgctcgtc gtctcgtcgc cgtgaccgcg cggcgccgcg
     3121 tcccgggtgg tgtcagtgcg gaggcttggt cgcgtgctcg accgcctgga tcggcagcca
     3181 cgtcagggtg gtctgcggcc gcgcgtccga ctgaccttcc agcgcatcga aggcggtgaa
     3241 cccgtaccgg gcatagagct cgacggcgcc cggcttggca tcgacgacca cgccggcgca
     3301 accgacctcg tcggccagct tcgcggccag ctggagcacg aagcggagca gctgggagcc
     3361 gaggccgatc gacctggcgg acgtgtcgac cgcgagccgg gcgagcccga gcaccgggat
     3421 cgggtaccgg ggcagcttct tgcggaggcg ctcggggaga tcctcgatgt cgatgtggcg
     3481 cggggccacc gtggcgaagc cgaggatccg gtcgccgtcg accgccacgt aggtcacgcc
     3541 gaggtgatgg cggaactggt tctggccggc gtagcggtgg aagaaccggt ccagggcttc
     3601 gtcgcccgaa tgaaacaccg agcgatcgtc gctggcccgg agcgacctaa tctccatcgc
     3661 gcatcagctt gcgcagcgcc ggtgtcggct ccgcggcctc ggcgcgacga agcaggtcct
     3721 cgccggtctt ccgcgacacg acgatccggg gatggacgac gtactccgcg ggcagctcgt
     3781 cgagcgcctg caggtggtgg agcagcgcct gctcgatcag gtggcccttc ttgacgccgg
     3841 tccgccggac gtgcttctcc atgagcccgc gcgtcgtctc ggagatctgg gccgagatct
     3901 gggtctcctt caccatggca gcatgtcctc acgaaaattc tacgttcttg tagcactcga
     3961 ggcaagcgcg ggtggggtcc ctgacgatgg ggcgccggta tcgcgccgga cccgcatcga
     4021 tcgatgcgca ccggttccca acgacgcgcc gcggcgctaa ggtcgcaagc gcgatggtca
     4081 agcccgcata caagcgcatc ctcctcaaga tctccggcga agcgctgatg gggcaccagg
     4141 ggtacgggat caaccccgag gtcctgaccc acgtggccgg cgagatcgcc gagaccgcgc
     4201 ggctcggcgt cgaggtcgcc gtggtggtcg gcggcggcaa catcttccgc ggcgtgtcgg
     4261 agtcgtcgcg cggcatggac cgggcgagcg cggactacgt cggcatgctc gccacggtga
     4321 tgaactcggt atcgctgcag gaggcgatcg agaagcaggg ggtggtgaca cgcgtgcaat
     4381 cggcgatccc gatgtcgcag ctcgccgagc cctacatccg gcgccgggcg atccgccacc
     4441 tcgagaaggg ccgggtcgtg atcttcggcg cgggcaccgg caacccgttc ttcaccaccg
     4501 acaccgcggc ggcgctgcgc gcgatggaga tcggcgccga gatcctgctc aaggcgacca
     4561 aggtcgacgg cgtgtacgac aaggacccgg ccaagcactc cgacgcacgg cgttacgcca
     4621 cggtgacctt caccgacgcg ctcaagcagg atctcaaggt gatggacgcg acggcgttcg
     4681 cgctgtgccg ggacaacgag atgcgcatca tcgtgttcga catgacgacg cgtggcaaca
     4741 ttcaccgggt ggtctgcggc gagccggtcg gaacgacgat cgtcaaggac aaccagcaga
     4801 cccggttcgc gaactagagg ccccccgagg acatcatgat caacgacatc accagcgata
     4861 ccgaagacgg catgaagaag gcggtcgact cgttcaagcg cgatctccag aagatccgca
     4921 ccggccgcgc caacaccgcg atgctcgacg gtatcaaggt cgactactac ggcacgccga
     4981 cgccggtgaa ccaggtcgcg acggtgcagg tggtcgacgc gcggctgatc accatcaagc
     5041 cgtgggagaa gtcgatgctt caggtgatcg acaaggcgat ccgggcgagc gacctcggga
     5101 tcaacccggt ggctgactcc gagctggtcc ggctgccgat cccgccgctg acccaggagc
     5161 gccgcaagga gctcgccaag gtggtcggca agcagaccga ggaggcgcgg gtcgcggtgc
     5221 ggtcggcgcg gcgcgacgcg atggacatgg tgaaggacgc cgagaaggac aagcagatca
     5281 ccgaggacga gcgcaagaac ggcgagaaga agatccagga gctgaccgac aagtacatcg
     5341 cgatggtcga cgacatcgcg aaggccaaag agaaagagat catggagctg tagcctgtcg
     5401 ggcgacgggg taagataccc gccgatgtcc ggtattccgg caggcgccgg ccagctgctc
     5461 ggcaagcgcc tcggcaagta cgagatcctc gcgctgctgg cgctcggcgg gacggcggag
     5521 atctacctgg cgcggatcgc cggtgcggcc gggttcgaga agtacgtcgt ggtgaagtgc
     5581 ctccacgacc acctcgcgga tgacaccgag ttcgtgaaga tgtttctcga cgaggcgcgg
     5641 ctcgcggcgc acctcgatca ctcgaacatc gtccagacca tggagctcgg cgagcacgag
     5701 ggccgctact acatggtgat ggagttcctc gccgggctgt cgctggcgat gatcgtgcgg
     5761 cgggtcggcg agcggttgcc gagcatgcgg gtccccgtgc cgctggtgct caacatcctc
     5821 gcccaggcca gcgcggggct gcactacgcg cacgagaagg cgatgaacgg caaggcgctg
     5881 aacatcgtgc accgtgatat ctcgccgcag aacctggtga tcagcttcga gggcgtggtc
     5941 aagctggtcg acttcgggat cgccaaggcc gagctgcgcg agacccggac gcggtcgggg
     6001 acgatcaagg gcaagttcgc gtacatgtcg cccgagcagt gcgtggccaa caacgtcgac
     6061 cggcggaccg acgtgttcgc gctcggcgtg atcggccacg agctg
//
LOCUS       DASVOR010000727         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_328411,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000727 DASVOR010000000
VERSION     DASVOR010000727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328411"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(885..1577)
                     /locus_tag="VF516_14370"
     CDS             complement(885..1577)
                     /locus_tag="VF516_14370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108912.1"
                     /translation="MGFVEAEGGWGFQLGELAYLPDEKPTRYKHPLVTGWSGGLTGGW
                     FVGNDLAVIGSYQYRTASTREGSITGVLDRIQGHIHYHTFALGVRMYRATGPGRLRAE
                     FAVGLAFPFHTELEYDYGPALALAGIRGTGTRIENYDLGYGAQAQIGYELPVAEIPYG
                     SLYVALALELRAFQSRNWGRSTELDNFVTDLAAMPPTATTATIDNENGAAPPRQYAVS
                     DVTLRLAIGARF"
     gene            complement(1863..>2152)
                     /locus_tag="VF516_14375"
     CDS             complement(1863..>2152)
                     /locus_tag="VF516_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108913.1"
                     /translation="PARARDRERRKQSPDSPHWVTSCPAGDANDPPAARSDALRSDGN
                     RLAGNPILQIRRRAIAGADHEIHASTLDLNDCRSRCIPLHCMRARVHIGSG"
BASE COUNT          366 a          730 c          734 g          322 t
ORIGIN      
        1 cggcgaccgg gcgatcgagc agcaagtcgt gcgcgaggta gacctggccc atcgcgccga
       61 caccgagcag ccggaccagg cggtactcgc cgaactcgtc gggagggctc cacgcacggc
      121 cgccggcacc cacctcccgc gacgggcgat gatcgcagct tgccggccgc gcaggctgaa
      181 cctcagagcg agccacgggc gatccactcc tgatcgagaa gcagtcgagc cgcagcgcag
      241 cgcaccaggc acctcctcat catcaatagt ggaccttact tcaagatcaa aggaagacgt
      301 cctcaaaacg aatctggcag acaaaattca catacaacca gggagcgaat ctgctgtgtg
      361 cggccgactg ctgcgtcatc gccggtgggc gtgagataca gtgtcggtga gattcactat
      421 cgaagcggcg gcgatgtcgg ccgatgtcag gccgacgaac cgtgcgagga cattcgcgcg
      481 cagccatgcg ctgcacgaac atcgacggtc cgccgcgcgg gccgtgacac cggcgcacgg
      541 tcgatccaga acacatgacc tccgcgacgc gcaccggcag gaagcatgta tttgctccgg
      601 cacagtctcc cacgcgctcc gctgcggccg gcgcgcgtgg ccggtcacga ccggtgtgcg
      661 tgggcgctca gcgacgccgc cctgacggcg gagcgcaacg cgagccctgc acgagcgcgc
      721 cccggatcct gttcgcgttc gcgtgacctg ggggcgcaat gtggcagtgc gggtacgccg
      781 agcgcgctcg tgcagcgcgt gccccgagcc tcatgacgcc ggtacgtcct gacgccgcgc
      841 ctcaccggcg gccgggatcg tgcgcccgag gcgcgaggtc gccgtcagaa ccgcgcgccg
      901 atggcgagcc gcagcgtgac atccgagacc gcatactgcc tcggcggggc cgcgccgttc
      961 tcgttatcga tcgtggcggt ggtcgccgtc ggcggcatcg ccgcgaggtc ggtcacgaag
     1021 ttgtcgagct cggtcgacct gccccagttg cggctctgga acgcgcggag ctcgagcgcg
     1081 agcgcgacgt acaggctgcc gtacgggatc tcggccaccg gcagctcgta gccgatctgc
     1141 gcctgcgcgc cgtagccgag gtcgtaattt tcgatcctcg ttccggtgcc gcgaatgccc
     1201 gcgagcgcga gcgccgggcc gtagtcgtac tcgagctcgg tgtggaacgg aaacgcgagg
     1261 ccgacggcga actcggcgcg cagccggccc ggtcccgtcg cgcgatacat gcgcacgccg
     1321 agcgcgaagg tgtggtagtg gatgtggccc tggatgcggt cgagcacgcc ggtgatgctg
     1381 ccctcgcggg tcgacgcggt ccggtactgg tagctgccga tcacggccag gtcattgccc
     1441 acgaaccagc ccccggtgag cccccccgac cagccggtga ccagcgggtg cttgtaccgg
     1501 gtcggcttct catcgggcag gtacgcgagc tcgccgagct ggaagcccca gccaccctcc
     1561 gcctcgacga atcccatttc ggacggaacg ggggcgtcgc cttccgcgcg gctcgctgca
     1621 ggccacgcga gggacagacc gatgattgtg gtgaagaacg gtcgcatatg catggcggcc
     1681 ggccgtgcaa gcagcacacc gggacggctg gttcgtggct ccgagcaccg accgcgcgtg
     1741 aacaaccatc tcctcgcgag cgcgggcgcc ggatcaccga gcacccacgc ggtgcgatgc
     1801 tgcgcatcct cgcatcaccg ggagtgcacg acctggcccg gatcctccgc catcactacc
     1861 gatcatccag agccgatatg cactcgcgct cgcatgcagt gcagtggaat gcagcgcgat
     1921 cgacaatcat tcagatccag ggtggacgcg tgaatctcgt gatcggcacc ggcgatcgct
     1981 cgtctgcgaa tctgcaggat cggattgccc gcgagccggt tgccatcgga gcgcagtgca
     2041 tccgatcgcg ctgctggagg gtcgtttgca tctccagcag ggcaggaggt cacccaatga
     2101 ggcgaatcag gagactgttt tctgcgctcg cgatcgcggg cgcgagctgg gt
//
LOCUS       DASVOR010000728         6532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_240663,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000728 DASVOR010000000
VERSION     DASVOR010000728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6532
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240663"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1868)
                     /locus_tag="VF516_14380"
     CDS             complement(<1..1868)
                     /locus_tag="VF516_14380"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108914.1"
                     /translation="MMAAGAKAAAGKPPRKPLVEPGTRVAHYEIIRALGRGGMGEVHL
                     ARDVRLGRLVALKFLIPSSPEVARMLLVEARATAKCKHENIVVIHDMNEYRGMPYLVL
                     EYLEGKSLNRLHAESRLPVIRMIEVIAAVVRALDHAHRVGIVHRDLKPDNIFVTTTGD
                     VKVLDFGIAKLVGSPASHAAQTPESPAVHDDETVITLSSKGPVGTRPYMAPEQWLGAD
                     IDHRTDIWAVGVILFRLVTGKYPYELDGPALMYAVAMPTEPVRSVRALVPDFHPELAA
                     IIDRCLRKAKDERFATARELLDALEPLLPHHAVAADDDRCPYPGLMAFQEADAERFFG
                     RDDQIARLVGRLATQPLISIVGPSGVGKSSFVRAGIVPALKREGPWGTIAMRPGRSPL
                     LALAALSDPARAHDLEYVRAVAQQMFEQPGYLGALLRWRAASHGCRVLLYIDQFEELY
                     TLVHDLRERVAFVTCLRAAADDPSSPVRVVLSLRSDFLDRAAEDRAFMASVTDGMHYL
                     MPLGHDGLRQALVRPAEQAGHVFESDELVGRMIADVALAPGALPLLQFAAARMWDARD
                     RSRRVMTAASYLAMGGIAGALAVHADAVLAELSPGRRRLAQSVFQRLVTADGTRAIV"
     gene            complement(1868..2827)
                     /locus_tag="VF516_14385"
     CDS             complement(1868..2827)
                     /locus_tag="VF516_14385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108915.1"
                     /translation="MTAPAAHADSKSATPWSRGVSEAAQKRALRLFQDGNAFLEDEKY
                     TEAVAKYEQALSAWDNPSIRFNLAICLINMRQPLVAWDHLQQALRFGDAPLGKRLYAE
                     GMRYVAVLESSLAQLTVRSTQPGIKVMVDGALALDGPGEHSMKLLAGKHQMVASRPGY
                     TTLSRALDLAAGTPDVERIALELEKVTEKVTVQRYNYERRWAWWVPWSLEGGAAALAL
                     VAAGFYVTASNEMVRYDQALLLRCPMGCSDADLASLKAQYDHARRNSGVAVGMWVGAG
                     AVAITGGVMAVLNRPFRVEGHPVTPSLAVSRDSVSAAISFALE"
     gene            3066..3854
                     /locus_tag="VF516_14390"
     CDS             3066..3854
                     /locus_tag="VF516_14390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108916.1"
                     /translation="MVGLARTPIVLVLLFGACVKSQAFPCGDLVCPESSVCAKGNTCV
                     SGSLVTACSGRAEGATCTLSELGNGTCQSGLCITGICGDGKVNGIEACDGADLGGRTC
                     VDFGSTDAKGLACTPDCSFDKSGCNGYCGDGHKGTEEQCDGSDFGGKTCTDFSPPGTT
                     NKFYRGGAPTCTIDCKINVSSCTGGWCGDGVKQFGEDCDGSDLGKPPPTCMSLGHPGA
                     ATPLACDPMKCTFTDDSCSCGPLIGICAPNIPTCVFNGGTYSCQ"
     gene            3965..4654
                     /locus_tag="VF516_14395"
     CDS             3965..4654
                     /locus_tag="VF516_14395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108917.1"
                     /translation="MGMSCGPSGDTCVYNDMIEACHLLADGAPCTVPGLPPNKCMSGV
                     CQASRCGDGRITGAEECDGTELASKTCQSLGFYEAGGLACGSDCKFDTKRCVGKCGDG
                     IKNGPEQCDKSDLGGATCFTAGYYKPLGLACKSDCTFDTTQCSGGHCGDGIINGLELC
                     DGTALPLVSGKTADCTALGFQGATTSLKCSKTCRYTTSSCLCTPTTRCNAKTQRCECS
                     KTGGCGCVAAK"
     gene            4638..4919
                     /locus_tag="VF516_14400"
     CDS             4638..4919
                     /locus_tag="VF516_14400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8108918.1"
                     /translation="MSLPSSTDPAPDQREVEAAIKSFLAAYHPNGARGAARIASVQNL
                     WLEINSLTLLELVVYVEQKFAITVRPIDFAPQNFSSLDAIARFVLRRRS"
     gene            complement(4933..5679)
                     /locus_tag="VF516_14405"
     CDS             complement(4933..5679)
                     /locus_tag="VF516_14405"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX8108919.1"
                     /translation="MRLADLQRQFQAAILEPSAPGSVDGLGLTIPGRVRVHHSHFWAR
                     MSEFIANWQPLLARYLGAEEMDQVVRRYIAAHPPRTVVATGVCAQLADFLRTAEPWSA
                     WPIVGELAAIDYRRALIRAGAEEPTVTKARLAAIDPAVIASIRFRLKQRSAVMTSRFQ
                     LDVSRLHLLARDTPLDARPVHRLVHLTGRRYATIELDPRSVRAFEPLVEGMTMSALDD
                     HLAGLGFDDGERRRFLDHVLDNDLLVAIQA"
     gene            complement(5670..>6532)
                     /locus_tag="VF516_14410"
     CDS             complement(5670..>6532)
                     /locus_tag="VF516_14410"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX8108920.1"
                     /translation="VAAVPDVIDLGVGVGLRPEHYPEFLRDDVTPARWVEVNAEKYLP
                     DRGGKRGAHLQVLEHVRARMPVVLHGTSMSLGSARDESEAYLARLKELCAIIEPAMVS
                     DHLCWTGIDGVHLYDLLPLPLVRESVELLVERIARAQDALGRRILVENLSTYMTFASS
                     EMSEWEFVSEVVQRADCHLLLDINNVHVSCFNHGWDAHAYLHGIPHDRVRQIHLAGYT
                     DHGRFKIDTHGEPVHEPVWELFRWYGDRYGTINPMIERDSDVPPWSELAGELRAMSAI
                     VSDLAEPRCA"
BASE COUNT         1023 a         2282 c         2268 g          959 t
ORIGIN      
        1 acgatcgcgc gcgtgccgtc cgcggtgacc aggcgctgga acaccgactg ggcgagccgg
       61 cggcgccccg gcgacagctc ggcgagcacg gcgtcggcgt gcaccgcgag cgcaccggcg
      121 atcccgccca tcgcgaggta gctcgcggcc gtcatcaccc ggcgcgagcg gtcccgcgcg
      181 tcccacatcc gggccgccgc gaactgcagc agcggcagcg cgcccggcgc cagcgcgacg
      241 tcggcgatca tccggccgac cagctcgtcc gactcgaaca cgtggccggc ctgctcggcg
      301 ggccgcacca gcgcctggcg cagcccgtcg tggccgagcg gcatcaggta gtgcatgccg
      361 tcggtcacgc tcgccatgaa cgcccggtcc tcggcggcgc ggtcgaggaa gtccgagcgc
      421 agcgacagca cgacgcggac cgggctcgac ggatcgtcgg ccgccgcccg caggcaggtc
      481 acgaacgcca cgcgctcgcg cagatcgtgg accagcgtgt agagctcctc gaactggtcg
      541 atgtagagca gcacgcggca gccgtggctg gccgcgcgcc agcgcaggag cgcgcccagg
      601 tagcccggct gctcgaacat ctgctgcgcg accgcgcgga cgtactcgag gtcgtgagcg
      661 cgggccggat cgctcagcgc ggcgagcgcc agcagcggtg agcgccccgg ccgcatcgcg
      721 atcgtgcccc acggcccttc gcgcttgagc gccggtacga tcccggcgcg gacgaacgac
      781 gacttgccga cgcccgacgg gccgacgatc gagatcagcg gctgggtcgc gagccggccg
      841 accaggcgcg cgatctggtc gtcgcggccg aagaaccgct cggcgtcggc ctcctggaac
      901 gccatcaacc cgggatacgg acagcgatcg tcgtcggcgg cgaccgcgtg gtgcggcagc
      961 agcggctcca gcgcgtcgag cagctcgcgc gcggtggcga accgctcgtc cttggccttg
     1021 cgcaggcagc ggtcgatgat cgcggcgagc tcggggtgga agtcggggac cagcgcccgc
     1081 acgctgcgca ccggctcggt cggcatcgcc accgcgtaca tcagcgccgg gccatcgagc
     1141 tcgtacgggt acttgccggt caccagccgg aacaggatca cgccgaccgc ccagatgtcg
     1201 gtgcggtggt cgatgtcggc gccgagccac tgctccggcg ccatgtacgg ccgggtgccg
     1261 accgggccct tgctgctcag cgtgatgacg gtctcgtcgt cgtggaccgc cggggattcc
     1321 ggcgtctgcg cggcgtggct cgccggactg cccaccagct tggcgatgcc gaagtcgagg
     1381 accttcacgt ctcccgtggt ggtgacgaag atgttgtcgg gcttgagatc gcggtggacg
     1441 atgccgacgc ggtgggcgtg atcgagcgcg cggaccaccg cggcgatcac ctcgatcatc
     1501 cggatcaccg gcagccggct ctcggcgtga agccggttga gcgacttgcc ctccaggtac
     1561 tcgagcacca ggtacggcat gccgcggtac tcgttcatgt cgtggatcac gacgatgttc
     1621 tcgtgcttgc acttcgccgt ggcgcgggcc tcgacgagca gcatccgcgc gacctcgggg
     1681 ctcgacggga tcaggaactt cagcgcgacc aggcggccga gccggacatc gcgcgcgagg
     1741 tggacctcgc ccatcccgcc gcggccgagc gcgcggatga tctcgtagtg cgcaacccgg
     1801 gtgccgggct cgaccagcgg cttcctcggc ggcttgccgg ccgcggcctt cgcgccggcc
     1861 gccatcatca ctccagcgcg aacgagatcg ccgcgctcac cgaatcgcgc gacaccgcca
     1921 gcgacggcgt gaccgggtgg ccttccaccc tgaacggccg gttcagcacc gccatcacgc
     1981 cgccggtgat cgccaccgca ccggccccca cccacatgcc gaccgccacg ccgctgttgc
     2041 gccgcgcgtg gtcatactgc gccttgagac tcgcgaggtc ggcatcgctg caccccatcg
     2101 ggcacctcag cagcagcgcc tggtcgtagc gcaccatctc gttgctcgcg gtgacgtaaa
     2161 agccggcggc cacgagcgcg agcgccgcgg cgccgccctc gaggctccac ggcacccacc
     2221 acgcccagcg ccgctcgtag ttgtagcgct gcacggtcac cttctccgtc accttctcca
     2281 gctccagcgc gatccgctcc acgtccggcg tgccggccgc caggtcgagc gcgcgcgaca
     2341 aggtggtgta gccgggccgg ctcgcgacca tctggtgctt gccggcgagc agcttcatcg
     2401 agtgctcgcc cgggccatcg agcgccagcg cgccgtcgac catcaccttg atcccgggct
     2461 gggtcgaccg caccgtcagc tgggcgaggc tcgactcgag caccgcgacg tacctcatcc
     2521 cctcggcgta cagccgcttg ccgagcggcg cgtccccgaa ccgcagcgcc tgctgcaggt
     2581 ggtcccatgc gacgagaggc tgccgcatgt tgatcaggca gatcgccagg ttgaagcgga
     2641 tgctgggatt gtcccaggca ctcaacgcct gctcgtactt ggcgaccgcc tcggtgtact
     2701 tttcgtcctc gaggaacgcg ttgccgtcct ggaacagcct cagcgctcgc ttctgcgccg
     2761 cctccgagac gcccctggac cacggcgtcg ccgacttgct gtcggcgtgg gcggccggtg
     2821 cggtcatggc gccgaccacc gacaaccaca cggcgatggc aactcccagc ctcactgcgc
     2881 caacggtagc acttgccatc gatcaaatgg gtatcggaca gccatggcgg gcccgttcga
     2941 gcggaacgtt gtgcagcgga atgcacaaag gcggctcgga tcacgcggat gccgcactcc
     3001 gtcatgacac atgacgcgga cggtcgagtg gctgtggcgg accggcgacc tgatacactc
     3061 ggttcgtggt gggcctcgct cgcacaccga tcgtcctggt tctgctgttc ggcgcatgcg
     3121 tgaagagcca ggcgtttccg tgcggcgatc tggtgtgtcc ggagagtagc gtgtgcgcaa
     3181 aaggcaacac ctgcgtctcg gggtcgctgg tcacggcgtg cagcggccgg gccgagggcg
     3241 cgacctgtac gctgtccgag ctcggcaacg gaacctgcca gagcggcctg tgcatcaccg
     3301 ggatctgcgg cgacggaaaa gtgaacggca tcgaggcctg cgacggcgcg gacctcggcg
     3361 gcaggacctg cgtggacttc gggtcgaccg acgccaaggg cctggcgtgc actcccgatt
     3421 gctcgttcga caagtccggc tgcaacggct attgcggcga cggccacaag ggcaccgagg
     3481 agcagtgcga tggcagcgac ttcggtggca agacctgcac cgatttctcg ccgcctggca
     3541 cgacgaacaa gttctatcgc ggcggcgcgc ccacctgcac gatcgactgc aagatcaacg
     3601 tctcgagctg caccggcggg tggtgcggcg acggtgtcaa gcagttcggg gaagactgcg
     3661 acggcagtga tctcggcaag ccaccgccca cctgcatgag cctcggccat ccgggagcgg
     3721 cgacgccgct ggcctgcgac ccgatgaagt gcacgttcac cgacgatagc tgctcatgcg
     3781 ggccgctcat tgggatctgt gccccaaata ttccgacctg tgtcttcaac ggggggacgt
     3841 actcgtgcca gtgaggttcg ccatgccaag accgatcgcg gctgtatgcg tgatgctggt
     3901 ggggttcgcg ctcgctgcgt gcgtccagag tcacgcgagc cgctgcggga gcctcacctg
     3961 cccgatgggg atgagctgcg ggccgtcggg cgacacctgc gtgtacaacg acatgatcga
     4021 ggcgtgccac ctgctcgccg acggtgctcc ctgcaccgtc ccggggctgc cgccgaacaa
     4081 gtgcatgagc ggcgtgtgcc aggcgagccg gtgcggcgac ggacgcatca ccggcgccga
     4141 ggagtgcgac ggcaccgagc tcgcgtcgaa gacctgccag tcgctcggct tctacgaggc
     4201 cggcggcctg gcctgcgggt ccgattgcaa gttcgacacg aagcgttgtg tcggcaagtg
     4261 cggcgacggc atcaagaacg ggcccgagca gtgcgacaag agcgacctcg gcggcgcgac
     4321 gtgcttcacc gccgggtact acaagccgct gggcctggcg tgcaagtcgg attgcacgtt
     4381 cgacaccacg cagtgcagcg gtggccactg cggcgacggc atcatcaacg gcctcgagct
     4441 gtgcgacggc accgcgctgc ccctggtgag cggcaagacc gccgattgca cggcgctcgg
     4501 attccagggc gcgacgacca gcctcaagtg ctcgaagacc tgccggtaca ccacgtcgag
     4561 ctgcctgtgc accccgacga cccgctgcaa cgcgaagacg cagcgctgcg agtgctcgaa
     4621 gaccggtgga tgtggctgtg tcgctgccaa gtagcaccga ccctgctccc gaccagcgcg
     4681 aggtcgaagc ggcgatcaag tcgttcctcg ccgcgtacca tccgaacgga gcgcgcggcg
     4741 ccgcgcggat cgcgagcgtc cagaacctgt ggctcgagat caactcgctg acgctgctcg
     4801 agctggtcgt gtacgtggag cagaagttcg cgatcaccgt gcggccgatc gacttcgcgc
     4861 cgcagaactt ctcgagcctc gacgcgatcg cgcgattcgt cttgcgccgg cgatcctgag
     4921 cccgatcgag tctcaggcct ggatcgcgac cagcaggtcg ttgtcgagca cgtggtcgag
     4981 gaaccgccgt cgctcgccgt cgtcgaaccc gagacccgcc aggtggtcgt cgagcgcgga
     5041 catcgtcatg ccttcgacca gcggctcgaa tgcgcggacc gagcgcggat cgagctcgat
     5101 cgtcgcgtac cggcgtccgg tcaggtggac gagccggtgc accggtcgag catcgagggg
     5161 cgtgtcgcgc gcgagcaggt gcaagcgcga gacgtcgagc tggaacctgg acgtcatcac
     5221 cgcagaccgc tgcttcaggc gaaaccggat cgacgcgatc acggccggat cgatcgcggc
     5281 gagcctggcc ttcgtgaccg tcggttcctc cgcgccggcg cggatcagcg cgcggcggta
     5341 gtcgatcgcg gccagctcgc cgacgatcgg ccaggccgac cacggctccg cggtgcgcag
     5401 gaagtccgcg agctgtgcac acacgccggt ggcgacgacc gtccgcggcg gatgcgcggc
     5461 gatgtaccgc cggaccacct gatccatctc ctcggcgccg aggtacctcg cgagcagcgg
     5521 ctgccagttg gcgatgaact cgctcatccg cgcccagaag tgcgagtgat gaacgcggac
     5581 gcgcccgggg atcgtcaacc cgagcccgtc gacgctcccg ggcgcgctcg gttcgaggat
     5641 cgcggcctgg aactggcgct ggaggtcggc taggcgcacc gcggctccgc gagatcggag
     5701 acgatcgcgc tcatcgcgcg gagctcgccc gccagctcgc tccagggtgg cacgtcggag
     5761 tcgcgctcga tcatcgggtt gatcgtgccg tagcgatcgc cgtaccagcg gaacagctcc
     5821 cacaccggct cgtgcacggg ctcgccgtgg gtgtcgatct tgaaccggcc gtggtcggta
     5881 tagccggcga gatggatctg ccgcacgcga tcgtgaggga tcccgtgcag gtaggcgtga
     5941 gcgtcccagc cgtggttgaa gcacgacacg tggacgttgt tgatatcgag cagcaggtgg
     6001 cagtcggcgc gctggacgac ctccgagacg aactcccact ccgacatctc gctggacgcg
     6061 aaggtcatgt aggtcgacag attctcgacc aggatccggc gcccgagcgc gtcctgcgcg
     6121 cgcgcgatcc gctcgacgag cagctcgacg ctctcccgga ccagcggcag cggcagcagg
     6181 tcgtagaggt ggacgccgtc gatccccgtc cagcacaggt gatcggacac catggcaggc
     6241 tcgatgatcg cgcagagctc cttcagccgt gccaggtacg cctcgctctc gtcgcgcgcc
     6301 gaccccagcg acatcgacgt gccatggagg accaccggca tgcgcgcgcg cacgtgctcg
     6361 agcacctgga gatgcgcgcc gcgcttgccg ccgcggtccg ggaggtattt ctccgcgttg
     6421 acctcgaccc agcgggcggg cgtgacgtcg tcgcgcagga actcggggta gtgctcgggg
     6481 cgaagcccga cgccgacgcc gaggtcgatg acgtcaggca ccgcagccac cg
//
LOCUS       DASVOR010000729         3542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415560,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000729 DASVOR010000000
VERSION     DASVOR010000729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415560"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF516_14415"
     CDS             <1..403
                     /locus_tag="VF516_14415"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8108921.1"
                     /translation="APARQPTAGDGRKVLVVDDNRDAAATLADLLEAMGYTTRVAHDG
                     PNALRVAAEFDPELALLDIGLPVMDGYELAQRLRGRPGGRPLRLVAVTGYGQEADKQR
                     TREASFDAHMVKPVHVDHLQGVIRRLFPPPG"
     gene            complement(406..705)
                     /locus_tag="VF516_14420"
     CDS             complement(406..705)
                     /locus_tag="VF516_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108922.1"
                     /translation="MTPYIHVSFQRIDRNRSIESAIHRWVARFEAMRFEVRRASATVE
                     PGGRGRTMVKLSLVLADGTSRTVDATRTDPYVAVSDAFRSVRQQLLAAPAGRLVA"
     gene            complement(1016..1372)
                     /locus_tag="VF516_14425"
     CDS             complement(1016..1372)
                     /locus_tag="VF516_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108923.1"
                     /translation="MRNLGTAAQAVRADRRNERCSETDVESEHTMTEATTRWDDLHGI
                     TDELQLKIHLAEMDARDRWQELQPQLAEIEHKIADAGGVASKTVEHELAELGAGLRHL
                     SGDLVMRLRGNYVRGW"
     gene            1440..2078
                     /locus_tag="VF516_14430"
     CDS             1440..2078
                     /locus_tag="VF516_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108924.1"
                     /translation="MRAGRGVTEQRVFVHYAGIRGKIRDGDILLFRGKSWISRIICRV
                     TGSPYSHAAIVSWWNDRLMVLEAVGKGIVVSRMSVVVHQYSGKVELWTTDEKLGRTDV
                     IRAAQRLLGKRYAPYKLIRNLARLVFGRPRHQEADPEAPPDDFVCSEFVSRVWRAGGI
                     DLVADAPDLYTKPSDIAKSPRLRKVGGVLRGQPTELTGDSLDRLPASAAGAR"
     gene            complement(2088..2867)
                     /locus_tag="VF516_14435"
     CDS             complement(2088..2867)
                     /locus_tag="VF516_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108925.1"
                     /translation="MAFFTLACAFHSTSRLLERILPQAIRALTVPTRHDFEVVLVADR
                     SRADVVAALLPRLDELGVDELRFRRTRRHSFSGAPSNNFHANHFITTNPYLITFTDDT
                     FVAKTDPEFDVLDAIASLFEHHPELVLVSKVDDHDEWDRPLVDVGPELEPGIRSVNRA
                     VDQLIAYDTARFLPIARRFGAFHRDTFVSQPGFEYQWENMVSHAGTTGGRKIAYPERW
                     PLRVRHADLRIEHGSMHGTGNEDVKLQCFARLLETQGLEKR"
     gene            complement(2889..>3542)
                     /locus_tag="VF516_14440"
     CDS             complement(2889..>3542)
                     /locus_tag="VF516_14440"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosyltransferase-related protein"
                     /protein_id="HEX8108926.1"
                     /translation="TVVESDTAPRWREVIEPYADHYLFAEKPTTFNKSWAVNAGIVNT
                     PGPTQVICILDADVLVDRDFIARNVARFTRPGTGGHLPYRNMTCLTAAATSWAIDARV
                     RGGVAELDPARLRGFQLRRPPGCCVWVRAEAFHRIGGMDERYEGWGGEDNDFAYRFDV
                     AAPLDHHDDWLLHMYHPPASLLRDDGELVNAHIPPLSWRPDQPIGALDRFAGPTPRA"
BASE COUNT          498 a         1204 c         1297 g          543 t
ORIGIN      
        1 cgcaccggcc cggcagccca ccgccggcga cggccgcaag gtgctcgtcg tcgacgacaa
       61 ccgggacgcc gccgcgacgc tcgcggatct gctggaggcg atgggataca ccacccgggt
      121 ggcgcacgac gggcccaacg cgctgcgggt cgccgcggag ttcgaccccg agctcgcgct
      181 gctcgacatc gggctgccgg tgatggacgg ctacgagctc gcgcagcggc tccgcggccg
      241 accgggcggt cggccgctgc ggctggtcgc ggtgaccggc tacgggcagg aggccgacaa
      301 gcagcgcacc cgcgaggcca gcttcgacgc gcacatggtc aagccggtcc acgtcgatca
      361 cctccagggc gtgatccgcc ggctgttccc gccgcctggc tgacgctacg cgaccagccg
      421 gccggccggc gccgcgagca gctgctggcg taccgaacga aacgcatccg agaccgccac
      481 gtacggatcg gtacgcgtgg cgtccaccgt tcgcgatgtt ccatccgcga gcaccagcga
      541 gagcttgacc atcgtgcgac ctcgcccgcc gggctcgacg gtggcgctcg ctcgtcggac
      601 ctcgaaccgc atcgcctcga agcgcgcgac ccagcgatgg atcgcggact cgatcgaccg
      661 atttcgatcg atgcgctgga agctcacgtg aatgtacgga gtcatggacc gagcgccatg
      721 cagaccttgc gccatctcat gcctggcccc tggcctgata aaggctcata ataactcgag
      781 tccgtcgtgg cgagatcgct cggtgagcgt gcgtatattt cgcacggtcg cacgcccgct
      841 cgcacggcgt cccccgaggt ggctgttgcg gtgacgtccg ggccatttcg ggatcgctgc
      901 gccgcgcgat tcccgggagg catcggctcg gtgcgcaagc gccgcgtggt gcgaccgccg
      961 cggcgcgtgg tctgcgcggc ctcgtgtcga ccgcgcggcg gatcgcctgc gcgccctacc
     1021 agcccctcac atagttgccg cgcagccgca tcaccagatc gccgctcagg tggcgcaggc
     1081 cggcgccgag ctcggccagc tcgtgttcca ccgtcttgct cgccacgccg ccagcgtcgg
     1141 cgatcttgtg ctcgatctcg gccagctgcg gctgcagctc ttgccagcgg tcgcgcgcat
     1201 ccatctcggc gaggtggatc ttgagctgga gctcatcggt gatcccgtgc aggtcatccc
     1261 agcgggtggt ggcttcggtc atggtgtgct cgctttccac gtccgtctcg ctgcatcgct
     1321 cgttccgacg gtcggcgcgc accgcctgcg cggccgtgcc gaggttgcgc acaacgtacg
     1381 ccgggccgtg cggcgtattc tgcgtgactc gcgcggcggc actgatctag gatggttctg
     1441 tgcgagctgg cagaggggtc acggagcagc gcgtgttcgt gcactacgcg gggatccggg
     1501 gcaagatccg cgacggcgat atcctgctgt tccgcggcaa atcgtggatc agccgcatca
     1561 tctgccgggt caccggctcg ccgtactcgc acgccgccat cgtctcgtgg tggaacgacc
     1621 gcttgatggt gctcgaggcg gtgggcaagg ggatcgtggt gtcgcgcatg tcggtcgtcg
     1681 tccaccagta ctccggcaag gtcgagctgt ggacgaccga cgagaagctg gggcgcaccg
     1741 acgtgatccg cgccgcccag cgcctgctcg gcaagcgcta cgcgccgtac aagctgatcc
     1801 gcaacctcgc gcggctggtg ttcgggcggc cgcgccacca ggaggccgat cccgaggcgc
     1861 cgcccgacga cttcgtgtgc tcggagttcg tcagccgggt gtggcgcgcc ggcggcatcg
     1921 acctcgtggc ggacgcgccc gacctgtaca ccaagccgag cgacatcgcg aagtcaccgc
     1981 gcctgcgcaa ggtcggcggc gtgctccgtg gccagcccac ggagctcacc ggcgacagcc
     2041 tcgaccggct ccccgcctcc gcggccggcg cgcggtagct cgatggctca gcgcttctcg
     2101 aggccctggg tctcgagcag ccgcgcgaag cactggagct tgacgtcctc gttgcccgtg
     2161 ccgtgcatcg agccgtgctc gatgcggagg tcggcgtggc ggacgcggag cggccagcgc
     2221 tcgggatagg cgatcttgcg cccgccggtc gtgccggcgt ggctgaccat gttctcccac
     2281 tggtactcga agccgggctg gctgacgaag gtgtcgcggt ggaacgcgcc gaaccggcgg
     2341 gcgatcggca ggaaccgcgc ggtgtcgtac gcgatcagct ggtcgaccgc gcggttgacc
     2401 gagcggatgc cgggctcgag ctcggggccg acatcgacca gcggccgatc ccactcgtcg
     2461 tgatcgtcga ccttgctgac cagcacgagc tcggggtggt gctcgaacag gctcgcgatc
     2521 gcgtcgagca cgtcgaactc cgggtcggtc ttggccacga acgtgtcgtc ggtgaacgtg
     2581 atcaggtagg ggttggtggt gatgaagtgg ttggcgtgga agttgttcga cggcgcgccc
     2641 gagaacgagt gccgccgggt ccggcggaac cgcagctcgt cgaccccgag ctcgtcgagc
     2701 cggggcagca gcgccgcgac gacgtcggcg cgcgagcgat cggcgaccag cacgacctcg
     2761 aagtcgtggc gggtcggcac ggtcagggcg cggatcgcct ggggcaggat gcgctcgagc
     2821 agccgcgacg tgctgtggaa cgcgcaggcc agggtgaaga aagccatcgg ggaacctcgg
     2881 ggcaggggtc aggcgcgggg cgtggggccc gcgaaccggt cgagtgcgcc gatcggctga
     2941 tcgggtcgcc agctcagcgg cgggatgtgg gcgttgacca gctcgccgtc gtcgcgcagc
     3001 agcgaggccg gcgggtggta catgtggagc agccagtcgt cgtggtgatc gagcggcgcg
     3061 gcgacgtcga agcggtacgc gaagtcgttg tcctcgccgc cccagccctc gtagcgctcg
     3121 tccatgccgc cgatccggtg gaacgcctcg gcgcgcaccc acacgcagca gccgggcggc
     3181 cggcggagct ggaagccgcg cagccgtgcg ggatcgagct cggcgacgcc gccgcggacc
     3241 cgggcgtcga tcgcccacga cgtcgccgcg gcggtcaggc acgtcatgtt gcggtagggc
     3301 aggtggccgc cggtgccggg ccgggtgaac cgcgcgacgt tgcgcgcgat gaagtcgcgg
     3361 tcgaccagca cgtcggcgtc gaggatgcag atcacctggg tcgggccggg cgtgttcacg
     3421 atcccggcgt tgaccgccca tgacttgttg aacgtcgtcg gcttctcggc gaacaggtag
     3481 tgatcggcgt acggctcgat cacctcgcgc cagcgcggcg cggtgtcgga ctcgaccacg
     3541 gt
//
LOCUS       DASVOR010000730         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211656,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000730 DASVOR010000000
VERSION     DASVOR010000730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211656"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1637
                     /locus_tag="VF516_14445"
     CDS             <1..1637
                     /locus_tag="VF516_14445"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX8108927.1"
                     /translation="ALEAWRQAGAPALVDAAQLDHAAEGPTEGFDEGFDEGVSRTPRP
                     ADPVYIAYTSGSTGRPKGIVQTHGGFHYLIRWFGARFGIEPGRAIAQWTSVGHDPCYA
                     EVFGALCSGATVSVVPRNLRQEPARVLRWLQDERISLVMTVPSFCREMLALVDGADAL
                     ADLEAILLTGEDLPVALAAAWRERFGDRTRLWNLYGPTESIVITSHPVDRVDAARLRV
                     PVGTPVDGCQIFVVNAEGQVCPTGVPGEIFVRSPFLATGYHGMPEATARGFVQNPLHG
                     DYPDRVYRTGDLARWLPDGTLEFLGRADGQVKIRGLRVEISEVEAVLGRRPEIEECAV
                     VFLDERGAQRLVAYAVARGEADAASVLAALGDELPRFMVPSALVWLDAMPRLANGKID
                     RQRLRGLAVQVPGRPYVAPEGALEQTIAGAFAELLRIEAERVGRHDDFFALGGHSLLA
                     ARLVNQLRDSQGVDLYVQHVFNHPTVAQLAQLVADRRAAGGVDDLEQQLAALLSRVGE
                     LSEDATERLLSREDTDDEPSTPELGAPRRRQRAHSK"
     gene            1646..>2175
                     /locus_tag="VF516_14450"
     CDS             1646..>2175
                     /locus_tag="VF516_14450"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX8108928.1"
                     /translation="MLDKLYADVTRLSPQKQKLLRTLMAQQGLSPSQLPIVAVERGAA
                     DYPLSFPQQRIWLLHRLAGSSLYNTPWILRLRGPLDRARLAAALARIVQRHEALRSCF
                     PFVDGSPRQRILPPAPVELPAVDLRAVSGAAEREARLHRELVAEIQRPLDLERGPIVR
                     ARLFALGTDEHVLVATT"
BASE COUNT          268 a          840 c          746 g          321 t
ORIGIN      
        1 gggcgctgga ggcctggcgg caggcgggcg cgcccgcgct cgtcgatgcc gcgcagctcg
       61 accacgccgc ggagggcccc accgagggct tcgatgaggg cttcgatgag ggcgtgagcc
      121 gcacgccgcg gcccgcggac ccggtctaca tcgcctacac ctcgggctcg accggcaggc
      181 ccaagggcat cgtccagacc cacggcggct tccactacct gatccgctgg ttcggcgcgc
      241 gcttcggcat cgagcccggc cgggcgatcg cgcagtggac ctcggtcggc cacgatccct
      301 gctacgccga ggtgttcggc gcgctgtgct ccggcgccac cgtcagcgtg gtgccgcgca
      361 acctccggca ggagccggcg cgcgtgctgc gctggctcca ggacgagcgc atctcgctgg
      421 tgatgacggt gccgagcttc tgccgcgaga tgctggcgct ggtcgacggt gccgacgcgc
      481 tcgccgatct cgaggcgatc ctgctcacgg gcgaggacct gcccgtcgcc ctggccgccg
      541 cctggcgcga gcgcttcggc gaccggacgc ggctgtggaa cctctacggt ccgaccgagt
      601 cgatcgtgat caccagccac cccgtcgacc gcgtcgatgc cgcgcggctg cgcgtcccgg
      661 tcggcacgcc ggtcgatggt tgccagatct tcgtcgtcaa cgccgagggt caggtgtgcc
      721 ccaccggcgt gccgggcgag atcttcgtgc gctcgccgtt cctcgccacc ggctaccacg
      781 gcatgccgga ggccaccgcg cgcggcttcg tgcagaaccc gctgcacggc gactacccgg
      841 accgcgtcta tcgcacgggc gatctcgccc gctggctgcc ggacggtacg ctcgagttcc
      901 tcggccgcgc cgacggccag gtcaagatcc gcggcctgcg cgtggagatc tccgaggtcg
      961 aggcggtgct cggccgccgg ccggagatcg aggagtgcgc cgtggtgttt ctcgacgagc
     1021 ggggcgcgca gcgcctggtc gcctacgccg tcgcccgcgg cgaggccgac gccgcctcgg
     1081 tgctcgccgc gctcggtgac gagctgccgc gcttcatggt gccgtccgcc ctggtctggc
     1141 tcgatgccat gccacgcctc gccaatggca agatcgaccg ccagcgcctg cgcggcctcg
     1201 ccgtccaggt ccccggccgg ccctacgtgg cacccgaggg ggcgctcgag cagaccatcg
     1261 ccggcgcgtt cgccgagctg ctccgcatcg aggccgagcg cgtcggtcgc cacgatgact
     1321 tcttcgccct cggcggccat tcgctgctcg ccgcccgcct ggtcaaccag ctgcgcgaca
     1381 gccagggggt cgacctctac gtccagcacg tcttcaacca ccccacggtc gctcagctcg
     1441 cgcagctcgt cgccgatcgc agggccgcgg gcggcgtcga cgatctggag cagcagctgg
     1501 ccgcgctgct gtctcgcgtg ggagagctct ccgaggacgc caccgaacgc ctgctgtcac
     1561 gtgaggacac cgacgacgaa ccatcgacac ccgagctcgg cgctcctcgg cgacgtcagc
     1621 gagctcacag caaatgagtc attccgtgct ggacaagctc tacgccgacg tcaccaggct
     1681 gtccccccag aagcagaagc tgctgcggac cttgatggcc cagcaggggc tgtcgccgtc
     1741 gcagctgccg atcgtcgccg tcgaacgcgg ggccgcggac tatccgctgt cgttcccgca
     1801 gcagcggatc tggctgctcc atcgcctggc cggctccagc ctctacaaca cgccctggat
     1861 cctgcgcctg cgcgggccgc tcgatcgcgc gcgcctcgcc gccgcgctgg cccgcatcgt
     1921 ccagcgccac gaggcgctgc gctcgtgctt tccgttcgtc gacggctcgc cgcgccagcg
     1981 catcctgccg ccggcgcccg tcgagctgcc ggccgtcgac ctgcgcgcgg tgtccggcgc
     2041 ggccgagcgc gaagcgcgcc tgcaccgcga gctcgtggcg gagatccagc gtcccctcga
     2101 cctcgagcgc gggccgatcg ttcgcgcccg cctgttcgcg ctcggcaccg atgagcacgt
     2161 gctggtcgcc accac
//
LOCUS       DASVOR010000731         1738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_357723,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000731 DASVOR010000000
VERSION     DASVOR010000731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357723"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VF516_14455"
     CDS             <1..1391
                     /locus_tag="VF516_14455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108929.1"
                     /translation="HAAQYLERIGEPDPGVRADHYREGDDLAAAAPLYCRAADQAAAG
                     HDLATALRRVELGLRCGPEGKLLGAMRGLQCYAHFWNDQWETAYPFGSEALELLRAGS
                     YRWCKTMGTMLTITSLSGKLYHFGRLVDAFAAVEVDPEARTAYVEAGALLAVMFAYLG
                     RRTQSRLFVERVEAIGGLQGDDDATAAGWVHFARVYHSRLLGSDPWHTLAISRQGSDA
                     FEKAGSTRNYVFLNNFTGIVQAELGDAEAGAATMRRVLLVAQKLNEALALSRTGVCLA
                     FILCFHGPISACDEAVQLADQTVKTRGINSIYLGMAHDVLGHAHLRNGRIEAAEREAR
                     RALEVLTSIPVLRLSASATLIRVLLATGRATEAKALADAGIIEAERIDGVGFAEVRLR
                     VAAAEARHAAGDVDGACAALRSALDHITARADRITDPTWRASYLGRVPDNLRARELAD
                     ALGIPGYPPSAA"
BASE COUNT          240 a          678 c          588 g          232 t
ORIGIN      
        1 gccacgccgc gcagtacctc gagcggatcg gcgagcccga tcccggcgtg cgcgccgatc
       61 actaccgcga gggcgacgat ctggccgccg ccgcgccgct gtactgccgc gccgccgacc
      121 aggcggccgc cggccacgac ctcgccaccg cgctccggcg ggtcgagctc ggcctgcggt
      181 gcggccccga gggcaagctg ctcggcgcga tgcgcggcct gcagtgctac gcgcacttct
      241 ggaacgatca gtgggagacc gcgtacccgt tcggcagcga ggcgctggag ctgctgcgcg
      301 ccggctcgta ccgctggtgc aagacgatgg gcacgatgct cacgatcacc agcctgtccg
      361 gcaagctcta ccacttcggc cggctggtcg atgcgttcgc cgccgtcgag gtcgaccccg
      421 aggcgcgcac cgcctacgtc gaggccggcg cgctgctcgc ggtgatgttc gcctacctcg
      481 ggcggcgcac ccagtcccgg ctgttcgtcg agcgcgtcga ggcgatcggc gggctgcagg
      541 gcgacgacga cgccaccgcc gccggctggg tccacttcgc gcgcgtctac cactcgcggc
      601 tgctcggcag cgacccgtgg cacaccctgg cgatctcgcg ccagggcagc gacgcgttcg
      661 agaaggccgg ctcgacccgc aactacgtct tcctcaacaa cttcaccggc atcgtgcagg
      721 ccgagctcgg cgatgccgag gccggcgccg cgacgatgcg gcgcgtgctg ctggtcgccc
      781 agaagctcaa cgaggcgctc gcgctgtcgc gcaccggcgt gtgcctcgcc ttcatcctgt
      841 gcttccatgg accgatctcc gcctgcgacg aggcggtcca gctcgccgat cagaccgtca
      901 agacccgcgg catcaactcg atctacctcg gcatggcgca cgacgtgctc ggccacgccc
      961 acctccgcaa cggccggatc gaggccgccg agcgcgaggc gcgccgcgcc ctcgaggtcc
     1021 tgacctcgat cccggtgctg cggctgagcg catcggccac gctgatccgc gtgctgctcg
     1081 cgaccggccg cgccaccgag gccaaggcgc tggcggacgc cgggatcatc gaggccgagc
     1141 ggatcgacgg cgtcgggttc gccgaggtcc ggctccgggt cgccgccgcc gaggcccgcc
     1201 acgccgccgg cgacgtcgac ggcgcctgcg ccgccctgcg ctcggcgctc gaccacatca
     1261 cggcgcgcgc cgaccggatc accgatccga cctggcgcgc cagctacctc ggccgcgtgc
     1321 cggacaacct gcgcgcgcgc gagctggcgg acgcgctcgg catcccgggc tacccgccgt
     1381 ccgccgcctg accgcgcagc gctcccgcgc agctcgcttc ccgctcgagt ggcgctgctt
     1441 ccgggccagc cgaccgaccg ggcggttgca gcgtcgatcg acgaaccggg gggctgacac
     1501 cacagcggaa cagaagacgg tcgctcggga cgaaacaggc gcgaaacacg gaggacgcgc
     1561 accgtgcaca cgcagcgctc ccggtgcgat ctcacgcacc gcatcgcccg ggatcggcgg
     1621 tctgtgccgt cgcgcacgga cgcgacgcgt tactccgatc ggatcccgct cctatacaaa
     1681 ataggtttgc catccgcacc gatcgatttc tgattgatgc gccgcccgcg ctctcctc
//
LOCUS       DASVOR010000732         6162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_7399,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000732 DASVOR010000000
VERSION     DASVOR010000732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6162
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7399"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..223)
                     /locus_tag="VF516_14460"
     CDS             complement(<1..223)
                     /locus_tag="VF516_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108930.1"
                     /translation="MRIITSRAEFDIESPACNVGVMQRGDIWWSRCDLAFEDFLWRGN
                     LRPVVLLSSAAAATLAVRAMWIVAPANDSR"
     gene            complement(520..1110)
                     /locus_tag="VF516_14465"
     CDS             complement(520..1110)
                     /locus_tag="VF516_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108931.1"
                     /translation="MTTFTPGESPQGVNRHTFEIDDYQSLNDALIRLPTCDPKVVFPA
                     QICTIPNSFKDGQNDGQSFTVIIGDSIEDITYSWNAALSLPTWMRYNLGYLWLPTELA
                     ANETIRSGLGKFINHCVTKMSIGNERIVKFSSVSLEQSELTDTVDAFSNQLWRSKLRQ
                     RLEQSSLSKHVAPNSFLTLSRGSDMFRWLQLRPLGI"
     gene            1812..2675
                     /locus_tag="VF516_14470"
     CDS             1812..2675
                     /locus_tag="VF516_14470"
                     /inference="COORDINATES: protein motif:HMM:NF025619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HEX8108932.1"
                     /translation="MVAAAMRTPFDQFGKQMVRDALEGRCSVEPDAEVPADTRRIDLW
                     VTPHEAGASLPDYLGLLGRITSSSVTLEFFHHTPSGEELRVCLIKHGEFRHFLSRRKT
                     LPPLPTQWVISSGRPDAGIDGLGFRPMTSGIRGIYESPPLQHTRLVVVSELPVTRDTL
                     LVRLLGAGAVVKQAIAELQALPAEASERRLALPILLRLRLAVPIDPAQQTSDDQEFLM
                     NTQDIVENWRREAIREGERKLLLRLLRRRFGTEVDGETERRVATAPAEQVEAWAERVL
                     SAGTLAELLAD"
     gene            complement(2811..3977)
                     /locus_tag="VF516_14475"
     CDS             complement(2811..3977)
                     /locus_tag="VF516_14475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108933.1"
                     /translation="MTWDHENKAGSGHHTRTSAVDTAGVGPGKRTLTEALGHPAGAPA
                     RAAEARHPDVDPGNDASPADVKLVAGRPPKAVGGAEASGAAEPEAEEARVHQVLDLDT
                     AIQRYAPLVFLAKGEKYGPASADAFIQNSRLRWSHDAGFPDDEIADKGKIDEAKLGGN
                     GKNNAAYTDQVESQVLPIKHGPHIRSDQDVRPNDGKGDGGNEGFFLDLDNSRRGSLGS
                     TKSPVYYEAKPGHYITYWFFYAYNDGPFPGGADNHEGDWERISVKLDASNRATAVAYY
                     QHEGSKQLPWGQVPKQGSHPIVYSAKGSHASYAAPGTYGLQKVAGVPLAKDHASRGQA
                     WATWKNLKNAQTQAWYGYGGAWGEVGNPEVDSVLHDETTGPQGPSHKKPAPDNF"
     gene            4209..5039
                     /locus_tag="VF516_14480"
     CDS             4209..5039
                     /locus_tag="VF516_14480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8108934.1"
                     /translation="MKLYAISDLHVGFEPNRHLWSTLGDHRDDWLAIAGDVGETEDHL
                     RFVLQIATSRFQRVLWVPGNHELWTLPDTDGGLRGEARYRRLVAICRDHGVLTPEDPY
                     PTWPGDGPPCVLAPMFLLYDYSFRPDHVAEDQAVAWARESGIECTDEVVLHPDPHPSR
                     AAWCRSRCALTRARLAGVPADCRTVLINHFPLRRDLARLPAIPRFSIWCGTRETESWH
                     VTYRAFAVVSGHLHIRSTRWIDGVRFEEVSLGYPRQRAPARAIEDFLREILPGPAIPS
                     "
     gene            complement(5183..5539)
                     /locus_tag="VF516_14485"
     CDS             complement(5183..5539)
                     /locus_tag="VF516_14485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8108935.1"
                     /translation="MGLPVADLDASVAFYTRALAPLGYQLVMRREQFAGFGVAGKPDF
                     FLGAGKPTDRLHVAFRARGRAEVCAFHAAALAAGGKDNGPPGVREMYHPNYYGAFVHD
                     PDGHNIEAVCHDPYLD"
     gene            5593..>6162
                     /locus_tag="VF516_14490"
     CDS             5593..>6162
                     /locus_tag="VF516_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108936.1"
                     /translation="MRTVLAVVFLACVLPRTAHAGRTFYGWLYGTEVVPERGVELQTW
                     VQDETDKYSTRNKETWLGWGPLVGITDELELGLPIELEWADVPLPNDMPGRRVSFTFK
                     RFGIEARYRLAPPDPVEAPALVPLVRVAVKRDVTARSNVRVEGDAVVSYQAGTVQVLA
                     DVGFTGDLASSASHLELHPGIGVSALVTGE"
BASE COUNT          998 a         2052 c         2048 g         1064 t
ORIGIN      
        1 cgcggctatc gttcgccgga gccacgatcc acatcgctcg aaccgcgagc gttgctgctg
       61 ccgccgacga aagcagcacc actgggcgga ggttccctcg ccagaggaag tcctcgaagg
      121 cgagatcaca tcgactccac cagatgtcac cccgctgcat aacgccaacg ttacacgcgg
      181 gcgattcgat gtcgaactcg gcccgtgatg tgatgatgcg cacgccgcgc ttgtcccgtg
      241 cgccagaccc acccgcgaac gccgccgacc cgcgtcaggc cgaccgcgag cgcgccgcgg
      301 gtccctacgc gcgtgctcag gatcacgcga cgcggagcca gctcggcgac gtgcggatcg
      361 aactcgtcac ggcagccgat cagcgacacg gcaaccgacg gtacggagcg ccccgacgac
      421 acacaagaga tggccatcga cgactaggga agccctcgac ggtctcaacg acctgccaat
      481 tcgatcgcaa gtcatccgaa tcaagtgatt cgttgaggtc taaataccca agggccgcaa
      541 ctgcagccag cggaacatat cagagccgcg actcaatgta aggaatgagt tgggcgctac
      601 gtgctttgaa agcgaagact gttccagtct ctggcgcaat tttgagcgcc aaagctgatt
      661 gctgaatgca tcaacagtat ctgttagctc tgattgttcg agtgagacag aggagaattt
      721 aacaatgcgt tcattgccaa tgctcatctt agttacgcaa tgattgatga actttcccag
      781 accagatctg atagtttcgt tagcagctaa ttccgttggc aaccaaaggt aaccaagatt
      841 gtaacgcatc caggtaggca gcgacaacgc agcattccac gagtaggtta tatcttcaat
      901 gctatcacca attatgacag tgaaactctg tccgtcattc tgtccgtcct tgaatgaatt
      961 cggtatcgta cagatctgag cgggaaacac caccttgggg tcacaggtgg gcagccgaat
     1021 gagcgcatca ttgagagact ggtaatcgtc gatctcgaag gtatggcgat tgacaccctg
     1081 cggggattcg ccgggagtaa aggtagtcaa ttcgtgcgct tcaacactgt cagatggcag
     1141 gggccgaacc gtggatctgg tcgcacacca ggatctccat cgaacctggc gagcaccgct
     1201 gctgttaaca gcgggtgccc gccgttacag ctgctgcggt tccagggcta ctgaaattga
     1261 ccggttatgc ggcaaagccg aaggcacatc cgaccacact tccggcatgc tgcgaacgcc
     1321 aagccaggcg ccgagcgatc gcgcgcgtgt cccgacctgc agggcggcgg ggcgaggaca
     1381 agcagctcca gctcgatcgc cacgtgggcc gtacgaccca agccctggtc gaggtgggcc
     1441 cgcggtggcg ctcgaacctg tgacaaggca tcgcgcgtcg ggaaggttgc gccatgaatt
     1501 gattcattaa cgcccggccg accgtgagcg cgccgctggc ccggggcgct cggccgaccg
     1561 caggagaaat cccgaccgag gttgtttgcg tgtccgattc tcggccaatc ggcatcatgt
     1621 gtccgatggg cggacactcc ggacagccgg acaagctggc ggcgaggatc gtgaggccgc
     1681 ataacgaacg gtctgtgcgc aggctgcgcc gccgcggcca gcgcagtcga agcattgggg
     1741 ctcgatcgcg cgcaacgctt ccaggcggct gcgttcatgc ccgtttcttt gccctcgcac
     1801 cagtccgtgc gatggtcgcc gcggcgatgc gtacgccgtt cgatcagttc ggcaagcaga
     1861 tggttcgcga cgcgctcgaa ggtcgctgct ccgtcgagcc cgacgcagag gtcccggccg
     1921 acacgcgtcg aatcgatctc tgggtcacac cccacgaggc gggcgcatcg ctgcccgact
     1981 acctgggcct cctggggcgc atcaccagca gctcggtcac gctcgagttc ttccaccaca
     2041 cgcccagtgg ggaagaacta cgcgtgtgct tgatcaagca cggcgagttc cggcatttcc
     2101 tgtcgcgacg caagacgctt ccgccgcttc cgacccagtg ggtgatctcg tcaggccgcc
     2161 ccgacgcagg catcgatggc ctgggattcc gtccgatgac ctccgggata cgcggcatct
     2221 acgagagccc gccgctccag cacacgcggc tcgtggtggt gagcgagctg ccggtaacgc
     2281 gcgacaccct gctcgtgcgc ctgctcggcg cgggggccgt ggtgaagcag gcgatcgccg
     2341 agcttcaggc gctgccggcg gaggcatccg agcgtaggct cgcgctgccg attctgctac
     2401 gattacgctt ggccgtgccg atcgaccctg cgcagcagac cagcgatgac caggagtttc
     2461 tgatgaatac ccaggatatc gtcgaaaact ggcgtcggga agcgatccgg gaaggcgagc
     2521 gcaagctgct gctgcgcttg ttgcggcgac gctttggaac cgaggtcgac ggtgagacgg
     2581 agcgccgcgt tgcgacggca cccgccgagc aggtcgaagc ctgggccgag cgggtgttgt
     2641 cggccggaac gcttgccgag ctcctggcag actgaacata gactgcaccg gcgagccgcg
     2701 cggctgagtt gcgcgaccga cagagcgaag gtcgagacgg ccgaggctga cctcctgcga
     2761 ggctgccgag ctccgaccgc ggatcggtcg gcggcctggc agccccgccg tcagaagttg
     2821 tcgggcgccg gcttcttgtg cgacgggcct tgcgggccgg tggtctcgtc gtggaggacc
     2881 gagtcgacct ccgggttgcc gacctcgccc cacgcgccgc cgtagccgta ccaggcctgc
     2941 gtctgggcgt tcttcaggtt cttccaggtc gcccaggcct ggccacggct cgcatgatcc
     3001 ttcgcgagcg gcacgccggc caccttctgc agcccgtacg tcccgggcgc ggcatacgag
     3061 gcatgcgatc ccttggcgct gtagacgatc gggtggctgc cctgcttggg gacctgcccc
     3121 catggcagct gtttcgagcc ctcgtgctgg tagtacgcga ccgccgtcgc gcggttgctt
     3181 gcgtcgagct tgaccgagat ccgctcccag tcgccttcgt ggttgtcggc gccgcccggg
     3241 aacgggccgt cgttgtaggc gtagaagaac cagtacgtga tgtagtggcc gggcttcgcc
     3301 tcgtagtaca cgggcgactt cgtggaaccc aggctaccgc gcctggagtt gtcgagatcg
     3361 aggaagaaac cctcgttgcc gccgtcgccc ttgccatcgt tgggccgcac gtcctggtcg
     3421 gagcggatgt gcgggccgtg cttgatcggc aagacctggg attcgacctg gtcggtatag
     3481 gcggcgttgt tcttgccgtt gcctcccagc ttcgcttcgt cgatcttccc tttgtcggcg
     3541 atctcgtcgt cggggaaccc cgcgtcgtgc gaccagcgca gccgcgaatt ctggatgaac
     3601 gcatccgcgc tggccggtcc gtacttctcg cccttggcga ggaataccag cggcgcatag
     3661 cgctggatcg ccgtgtcgag gtcgagcacc tgatggaccc gcgcctcctc ggcctccggc
     3721 tccgcggcgc ccgacgcctc ggcgccgccc accgctttcg gcggcctgcc ggcgaccagc
     3781 ttgacatccg ccggcgatgc gtcgtttccg gggtcgacgt ccgggtggcg cgcctcggcg
     3841 gcccgggccg gtgcaccggc gggatggccg agcgcctcgg tcaaggttcg cttgccgggg
     3901 ccgacaccgg cggtgtccac cgcgctcgtt cgcgtgtggt ggcccgatcc ggccttgttc
     3961 tcgtggtccc aggtcatcgt gcctcccgat ggtgagcgat ggacaccgga cacgccgcct
     4021 cgtgcgtgga acacagcaga cgtatcatgg gtcggcgccg cctcgtccat cgtccggcgg
     4081 cgaagtgcgg ctcactgcgc ggagcgccac ggtcggggga gaaaatcgcc cgctcgcacg
     4141 tatcctgtgg atcgacgcgc aggttccctg gcgcatagac cacacgatct tcggcgatca
     4201 taggcgcgat gaagctgtac gcgatcagcg atctgcacgt cgggttcgag ccgaaccgcc
     4261 acctgtggtc cacgctcggc gaccaccgcg acgactggct ggcgatcgcc ggcgacgtcg
     4321 gcgagaccga ggaccatctg cggttcgtgc tccagatcgc cacctccagg ttccagcgcg
     4381 tgctctgggt gcccggcaac cacgagctgt ggacgctgcc cgacaccgac ggcggcctgc
     4441 gcggcgaggc gcgctaccgc cgcctggtcg cgatctgccg ggaccacggc gtgctgacgc
     4501 cggaggaccc ctacccgacg tggccgggcg acggtccgcc gtgcgtgctc gcaccgatgt
     4561 tcctgctcta cgactactcg ttccgccccg atcatgtcgc cgaggaccag gcagtcgcgt
     4621 gggcgcggga gagcgggatc gagtgcaccg acgaggtcgt cttgcatccc gacccccatc
     4681 ccagccgcgc cgcctggtgc cgctctcgct gtgccctcac ccgcgcgcgt ctcgcgggcg
     4741 tgccggccga ttgtcgcacg gtcctcatca accacttccc gctccgtcgc gacctcgcac
     4801 ggctcccggc gatcccgcgg ttctcgatct ggtgcggcac ccgcgagacc gagtcctggc
     4861 acgtcaccta ccgcgcgttc gccgtggtct ctggacacct ccacatccgc tcgacgcgat
     4921 ggatcgacgg ggtccggttc gaggaggtct cgctcggtta cccgcgccag cgagctccgg
     4981 cgcgggcgat cgaggatttc ctgcgcgaga tcctacccgg tcccgccatc ccgagctgaa
     5041 ccggtcccac gtacgcgtgc tcgggccagg tcgacggttc cagcgctgca ccttcggtga
     5101 ggcgccatgc atcccgttgc ccgagcgtcc aggccggtgc gatgctcctc acgcgtagcc
     5161 ggcggcgtgg tggatgatct gatcagtcga ggtacggatc gtggcacacc gcctcgatgt
     5221 tgtgcccgtc gggatcgtgg acgaacgcgc cgtagtaatt cgggtggtac atctcgcgga
     5281 cgccgggcgg gccgttgtcc ttgccgccgg cggcgagcgc cgcggcgtgg aacgcgcaga
     5341 cctcggcgcg gccccgggcg cggaacgcga cgtgcagcct gtcggtcggt ttgccggcgc
     5401 cgaggaagaa gtccggcttg ccggccacgc cgaacccggc gaactgctcc ctgcgcatca
     5461 cgagctggta gcccagcggt gcgagcgccc gtgtatagaa cgccacactc gcgtcgagat
     5521 ctgcgaccgg aagccccatg tgatcgatca tgggcgcgac gataccccac agcgttaggc
     5581 taggatgccg tggtgcgtac cgtcctggcc gtcgttttcc tcgcgtgtgt cctgccgcgg
     5641 accgcacacg caggccgtac cttctacggc tggctctacg gcacggaggt ggtgccggag
     5701 cgcggggtcg agctgcagac ctgggtccag gacgagaccg acaagtacag cacgcgcaac
     5761 aaggagacct ggctgggctg ggggccgctg gtcggcatca ccgacgagct ggagctcggg
     5821 ctgccgatcg agctcgagtg ggcggacgtg cccctgccga acgacatgcc gggccggcgg
     5881 gtgtcgttca cgttcaagcg gttcggcatc gaggcgcgct atcgcctcgc gccgccggac
     5941 ccggtcgagg cgccggcgct ggtgccgctg gtccgggtcg cggtcaagcg cgacgtcacc
     6001 gcgcgcagca acgtgcgggt cgagggcgac gccgtggtgt cgtaccaggc cgggacggtc
     6061 caggtcctcg ccgacgtcgg gttcaccggc gacctcgcgt cgagcgcgtc ccacctggag
     6121 ctgcatcccg gcatcggggt cagcgcgctg gtcaccggcg ag
//
LOCUS       DASVOR010000733         3149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_474772,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000733 DASVOR010000000
VERSION     DASVOR010000733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474772"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VF516_14495"
     CDS             <1..367
                     /locus_tag="VF516_14495"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HEX8108937.1"
                     /translation="PPPSERPRREARPQPARPDREPREHPKLCEHRPAREPREQTVEG
                     SAWFTINIGRSKNADPKWLIPLLCRRGNIAKSEIGKIQILMRETRVEIRGDASAQFAR
                     SVRKPDDRDRNIHIEPLDA"
     gene            complement(439..1485)
                     /locus_tag="VF516_14500"
     CDS             complement(439..1485)
                     /locus_tag="VF516_14500"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8108938.1"
                     /translation="MAIELCSLRAELRSAGVFEHREARSWGKLGLMAAALAACLVGIA
                     TWGVVAAIALVPVAGVLCTSIAMCGHEGSHKSFSESPRRNALLVYLVFPLFTGLGSLY
                     WHNKHDRLHHGHPNVEGMDPDIKPFPFVSSLGDHQRCNRGERWFQRNFQRYAFWPMSL
                     LMAIGMRRSSMAYLGTYARTKGIDRAWLVEVACMVVHYTCWIVIPTIVWGPFAGLLTY
                     TAIWSVVGICLALVFAPAHIGLPVVNDQYRDWLHQIETTRNLELPRVVSIFFIGLDYQ
                     IEHHLFPKIPHMNLPKAAAITRAWCARAQIPYRSEPYLHALGDAARFIRDAWAREAIS
                     PVDMRGVLRATVLD"
     gene            1574..1903
                     /locus_tag="VF516_14505"
     CDS             1574..1903
                     /locus_tag="VF516_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108939.1"
                     /translation="MHAIVSSRAGSSSEDLPVLGWRYREPYSTKATFITLAIEDGARS
                     EILRDRMTHAKICRSAFDGYERGPNWIETCGEVAKLQIARRAPAVEVVELGTVRATAG
                     AISDGTL"
     gene            complement(2096..2251)
                     /locus_tag="VF516_14510"
     CDS             complement(2096..2251)
                     /locus_tag="VF516_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108940.1"
                     /translation="MNVTTKLTWYGSRPATSLGIRFVEFVGGHVGYAVDPVAFAAELA
                     PLLTAPP"
     gene            complement(2663..2971)
                     /locus_tag="VF516_14515"
     CDS             complement(2663..2971)
                     /locus_tag="VF516_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108941.1"
                     /translation="MALEDEEVRHHAAREADSKATTLSPGKRTLTEAVGMQLSANASG
                     AAPTNHTDPGAIQQAAAQGLAGGDATLPHSHRATRDRDLFFHHEVRSGRWFPTPRTRY
                     "
BASE COUNT          551 a         1028 c         1073 g          497 t
ORIGIN      
        1 gccgccgccg tcggagcggc cgcgccgcga ggccaggccg cagccggccc ggccggaccg
       61 cgaaccgcgc gagcacccga agctgtgcga gcatcggccg gcccgcgagc cgagggagca
      121 gaccgtcgaa ggatcggcgt ggttcacgat caacatcggg cgctcgaaga acgctgatcc
      181 gaagtggctg atcccgctcc tgtgccgtcg cggcaacatc gcgaagagcg agatcggcaa
      241 gatccagatc ctgatgagag agacccgcgt cgagatccgc ggcgatgcca gcgcgcagtt
      301 cgcccggtcc gtccgcaagc cggacgacag ggaccgcaac atccacatcg aaccgctcga
      361 cgcctgacgg cgtcagcgcg cgccaccccg cgagcaggtc atgctcgtgg cgcgatgccg
      421 ctcaggagcg gctcggtgct aatcgaggac ggtcgcgcgg agtaccccgc gcatgtcgac
      481 ggggctgatc gcttcgcggg cccacgcatc gcgaatgaac cgggcggcgt cgccgagcgc
      541 gtgcaggtac ggctcgctcc ggtagggtat ctgggcgcgc gcgcaccacg cacgcgtgat
      601 cgccgccgcc ttcggcaggt tcatgtgggg gatcttcggg aacaggtggt gctcgatctg
      661 gtagtcgagc ccgatgaaga agatggacac cacgcgcggc agctcgaggt tgcgcgtcgt
      721 ctcgatctga tggagccagt cccggtactg atcgttgacg accggcaagc cgatgtgcgc
      781 cggcgcgaat acgagcgcga ggcagatccc gacgaccgac cagatcgcgg tataggtcaa
      841 cagacccgca aacgggcccc acacgatcgt cgggatgacg atccagcacg tgtagtggac
      901 caccatgcac gcgacctcga cgagccaggc acggtcgatt cccttggtgc gggcataggt
      961 tccgaggtaa gccatcgacg accgccgcat gccgatcgcc atcaacagcg acatcggcca
     1021 gaacgcgtag cgctggaagt tgcgctggaa ccagcgctcg ccgcgattgc agcgctggtg
     1081 atcacccagc gacgacacga acggaaacgg cttgatatcg gggtccatgc cctcgacgtt
     1141 ggggtgaccg tgatggagcc ggtcgtgctt gttgtgccag tacagcgagc cgagcccggt
     1201 aaacaggggg aacacgaggt agacgaggag cgcgtttcgc cgaggcgatt cggagaagct
     1261 cttgtggctg ccttcgtggc cgcacatcgc gatcgacgtg cacagcactc ccgcgaccgg
     1321 gacgagggcg atcgccgcga cgacccccca cgtcgcgatg ccgacgagac acgcggccaa
     1381 cgcggccgcc atcaacccga gcttgcccca gctcctcgcc tcgcgatgct cgaacacacc
     1441 agccgagcgc agctccgccc gtaggctaca cagctcaatt gccatccggt gtggaccatc
     1501 gcccgaattc gtgatccttc gcgagcttgc tatgtcacga atcggtcacg taagccgggc
     1561 ctcgaggtag ccggtgcacg ccatcgtaag ctcgcgcgcc ggctcgtcct cggaggacct
     1621 gccggtgctc ggctggcgct accgcgagcc gtacagcacc aaggccacgt tcatcacgct
     1681 ggcgatcgag gacggtgccc ggtcagagat cctccgagac cgaatgacgc acgcgaagat
     1741 ctgccgcagc gcgttcgacg gctacgaacg cgggccgaac tggatcgaga cctgcggcga
     1801 ggtcgccaag ctgcagatcg cccggcgcgc gccggcggtg gaggtcgtgg agcttggtac
     1861 agtccgtgct acagctggcg caatctccga cggaacgttg tagggggagg cggcgggagt
     1921 cgaatcctgc tggcgatgcg ctccacgacg ttccgatcgt ggattctcgc gcactttgcc
     1981 tggtcgaggg taccacgaac gccatggagc gcagcggact ccgttcattc cccctctgtt
     2041 ctggacgttc tggctcggcc tcgaccatgt gtggcgagta cacggtcttc gcggatcacg
     2101 gcggcgcggt cagcagcggc gcgagctcgg cggcaaacgc gacgggatcg acggcatagc
     2161 cgacgtggcc gccgacgaac tcgacgaacc ggatgccgag cgatgtcgcg ggccgagacc
     2221 cgtaccaggt tagtttcgtt gtgacgttca tctcattgac gtccggttca gaccagacat
     2281 gtctgtactc cggacagtcc gtggcgcgct acggccagaa actaacccgg tggggagctt
     2341 tcgcgagata atctcgacgc ccctcaacag gagtccgatc gatgacaccc gtgcggaagc
     2401 gagccaccga aggttgatct tgcgacgaga ggagctgcga cgtctcgacc ccagccacct
     2461 ccggctggta gagggtggtg gccagacgga ttcggccgga ccctgcaccg aaagctgcat
     2521 cggctgcact atcaccacgc tggcggccag ccttggctgt gacgcgtagc ctcgggcctg
     2581 tgaggttcga ggcgagactg tcgtgagcag tcgcgtcatc gtgttggcgt cgtgcgcagc
     2641 gcggtgacgc tcatgcctgc ggtcagtagc gcgtgcgtgg cgtcgggaac cagcgacccg
     2701 agcgtacttc gtggtgaaag aacaggtcgc gatcccgagt cgcgcggtgt gagtggggca
     2761 gtgtggcatc tccaccggcc agtccctgag cggcggcttg ctggatcgcg ccgggatcgg
     2821 tgtggttcgt tggcgccgca ccggatgcgt tggcagagag ttgcatgccc accgcctccg
     2881 tgagcgttcg cttgccgggg gagagagtag tcgctttgct gtccgcctcg cgtgccgcgt
     2941 gatgtcgaac ttcttcatcc tcgagcgcca ccgccgcggc ttcacgaact acgcgacgcg
     3001 ttgaccgcag aatcgcgagc tctcgcgagc aggacgtcgc gctcgcgctc gttgcgggtc
     3061 agctccgccg cgcgctgaaa ttccgcgcgc gcctcgtcgc cgcgtccgag cttcgcgagc
     3121 aggtcgccgc gcaccgccgg cagcaggtg
//
LOCUS       DASVOR010000734         1630 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_474782,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000734 DASVOR010000000
VERSION     DASVOR010000734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474782"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..1408
                     /locus_tag="VF516_14520"
     CDS             278..1408
                     /locus_tag="VF516_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108942.1"
                     /translation="MERVVPIHSQFSLLDLSRTAGAIDAAEHERVDVAFIGTSAVWFG
                     FEPRRFDAELAARGHPLTSFNFGLVPTSVRLQRFFADAICEAHRARGTRWTMMIYEIG
                     PADFLDKVVAHDVTDPSIAAYEAQVAAMMFRPAELVSELRRDPGGTLRALLAKPLGHR
                     GPAAVLEAVSTYLYEDLEPEPWRKPVPELLAAVRPFWDVHRRTTWLPEARGFLPVGPE
                     ARALYDEIMRLNHTEKELAKQRVMVTEDLGLLPMEVNAAAVERLIEQIRVLARCPERV
                     VLVVMPISPNIMRVMRPEARRRLADVIANVQRNTGYPVLDFVDPPELGLDDYYDAEHL
                     SLTVSAGKFSSLLADRVADLLDHPARPAGGAASPGKPDLRDR"
     gene            1405..>1630
                     /locus_tag="VF516_14525"
     CDS             1405..>1630
                     /locus_tag="VF516_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108943.1"
                     /translation="MTFTTIYFALFLTAFVVLFHVARRFPARARQLVVVANIAFYATW
                     GVAYVPLLLGSSLLAFVAARRIAAARAPVQR"
BASE COUNT          218 a          575 c          571 g          266 t
ORIGIN      
        1 cgcacagcgc gaagtacatc cggcgcatgt ccacgatctc gagctggtgc ccgtcgtgat
       61 cgtgaagggc gatggcgccg tcgccggcaa cgcgcgcccg ctgtgcgaga tccacggggg
      121 cgttcgtagc atccatcgat cccggatcgc aagctcctat gttagcgtgt tcgccgtgag
      181 cgagactcgc gagcccgcgg tcgtgatgcg cgcagcaggc actgcggtgg cggtcctcgc
      241 actggcattg ttggcggcac gcggcttcgc gcagtgtgtg gagcgcgtcg tgccgatcca
      301 cagccagttc tcgctgctcg atctgagccg cacggccggc gcgatcgacg ccgcggagca
      361 cgagcgcgtc gatgtggcct tcatcggcac atcggccgtg tggttcggat tcgagccgcg
      421 ccggttcgac gcggagctgg ccgcgcgcgg ccaccccctg acgtcgttca acttcgggct
      481 ggtgccgacg tccgtgcggc tccagcggtt cttcgccgat gcgatctgcg aggcccatcg
      541 agcgcgcggc acgcgctgga cgatgatgat ctacgagatc gggcccgccg acttcctgga
      601 caaggtggtc gcgcatgacg tcaccgaccc gtcgatcgcg gcctacgagg cgcaggtcgc
      661 ggcgatgatg ttccggccgg cagagctcgt ctccgagctg cgccgcgatc ccggtggcac
      721 gctgcgcgcg ctgctcgcga agccgcttgg ccatcgcggc ccggcggccg tgctcgaggc
      781 cgtgtcgacg tatctctacg aggacctcga gccggagccg tggcggaagc ccgtaccaga
      841 gctgctcgcg gcggtcaggc ctttctggga cgtgcatcgc cggacgacgt ggctccccga
      901 ggcccgcggc ttcttgccgg tcggcccgga ggcgcgcgcc ttgtacgacg agatcatgcg
      961 gctcaaccac accgagaagg agctggccaa acagcgcgtc atggtgaccg aagacctcgg
     1021 cctgctgccg atggaggtca acgccgccgc cgtcgagcgc ctcatcgagc agatccgcgt
     1081 gctcgcgcgg tgccccgagc gcgtcgtgct cgtggtgatg ccgatcagcc cgaacatcat
     1141 gcgcgtgatg cggccggagg cgcgcaggcg gctcgcggac gtgatcgcga acgtccagcg
     1201 caacaccggg tatccggtgc tcgacttcgt ggaccccccc gagctggggc tcgacgacta
     1261 ctacgacgcc gagcatctct cgctgacggt gagcgccggg aagttcagca gcttgctcgc
     1321 ggaccgcgtg gccgacctcc tcgaccaccc cgcgcgacca gccggcggcg ccgcctcgcc
     1381 cggcaagccg gacctccgcg accgatgacg ttcacgacga tctacttcgc gctgttcttg
     1441 accgcgttcg tcgtgctgtt ccacgtcgcg cgcaggttcc cggcgcgggc gcggcagctc
     1501 gtcgtggtcg cgaacatcgc gttctatgcg acctggggcg tcgcgtacgt gccgctgctg
     1561 ctcggctcga gcctgctggc gttcgtggcg gcgcgccgca tcgccgccgc gcgcgcgccg
     1621 gtgcagcgcc
//
LOCUS       DASVOR010000735         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211730,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000735 DASVOR010000000
VERSION     DASVOR010000735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211730"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VF516_14530"
     CDS             <1..625
                     /locus_tag="VF516_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108944.1"
                     /translation="ERLAPGIELHVERGRLFARSVPAAGEPIELIPLGGDRFRVAAAS
                     GSHLRLGRDREGRRIVVAGWQYLVEEPRARSLAFAVAPGICLAILMTGLVLPFGVLRR
                     RVGPVPGSGWPLCVTAACVAVPRLFLAALDPFTLGELNLYTAGIFVGTLVFAAGSAGS
                     LVQALLWLPLPGSVVGKLHRLAFGLTACCATAYLAAYGIIGIRLWSY"
     gene            770..1150
                     /locus_tag="VF516_14535"
     CDS             770..1150
                     /locus_tag="VF516_14535"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8108945.1"
                     /translation="MRGRKTIVVLDDEPGFCDTVKDALEEEGYAVETAMDGRSGLELL
                     RGLRTTPCLLLLDLAMPVLDGNAVYRELLADPALASIRVVVATSDPTRAPRGVQVLAK
                     PLSLARLIGLVRTCCADRPDDAPR"
     gene            complement(1160..>1975)
                     /locus_tag="VF516_14540"
     CDS             complement(1160..>1975)
                     /locus_tag="VF516_14540"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8108946.1"
                     /translation="RHWLITYYPVRGPAGEVSQVGVVVVDVTREQRARVELERTIEYN
                     EKFTAMLGHDLRNPLNTIATAAQLLQRRATAPEIGRPAARIVSAAERMSGMIAQLLDL
                     ARVRVTGLELAPRPLDLAELFGAVLNELIELHPGAQVDLAAAGALHGRWDGDRLTQLL
                     SNVVGNALAHGEPGAPVQVQLDGSDPQRVAIRIANRGAIADDVLATLFEPFRGIHHRC
                     ERTRGLGLGLYISKEIVHAHRGRIAVQSTRDAGTCFEIELPRDVRPAVRPGPD"
BASE COUNT          228 a          682 c          767 g          298 t
ORIGIN      
        1 cgagcgcctg gcacccggga tcgagctcca cgtcgagcgc ggccggctgt tcgctcgctc
       61 cgtgccggcg gccggcgaac cgatcgagct gatcccgctc ggcggtgatc ggttccgcgt
      121 cgccgcggcg agcggcagcc atctccggct gggacgcgac cgcgagggcc ggcggatcgt
      181 ggtcgccggc tggcagtacc tggtcgagga gccgcgcgcg cggtcgctgg cgttcgccgt
      241 cgcgcccggc atctgcctgg cgatcctgat gaccggcctg gtgctgccgt tcggcgtgct
      301 gcggcggcgg gtcgggccgg tgccggggtc cggctggccg ctgtgcgtga ccgcggcatg
      361 cgtcgcggtc ccccggctgt tcctcgccgc gctcgatccg ttcaccctcg gcgagctcaa
      421 cctctacacc gcgggcatct tcgtcgggac gctggtgttc gcggccggct cggccggcag
      481 cctggtccag gcgctgctgt ggctgccgct gccggggtcg gtcgtgggca agctgcaccg
      541 cctggcgttc gggctcaccg cgtgctgcgc gaccgcgtac ctcgcggcct acgggatcat
      601 cgggatccgg ctgtggagct actgaccgac atcggcggca ctcgatcgct ttccgggaat
      661 cgccaggagg tgccgatctt ttgcgggtcc cgggtccgga gttcgtgtcg ggcgcctgac
      721 acggtggcgc gcccgaccgg atcgcgaaaa aggtgcaaca ctgacgacga tgcgtggacg
      781 taagaccatc gtcgtcctgg atgacgagcc ggggttttgc gatacggtga aggacgccct
      841 cgaagaggag ggttacgcgg tcgagaccgc gatggacggg cgcagcggcc tggagctctt
      901 gcgcgggctg cgcacgacgc cgtgcctcct gctgctcgac ctcgccatgc cggtgctcga
      961 tggcaacgcg gtgtaccgcg agctgctggc cgatccggcg ctcgcgtcga tccgcgtcgt
     1021 ggtcgcgacg tcggacccga cgcgcgcgcc gcgcggggtc caggtgctgg ccaagccgct
     1081 gtcgctcgcg cggctgatcg gcctcgtccg cacctgctgc gcggaccggc cggacgacgc
     1141 gccgaggtga cgcgggcggt cagtctggcc cgggccggac cgccggccgg acgtcccggg
     1201 gcagctcgat ctcgaagcag gtgccggcgt cgcgcgtcga ctgcacggcg atgcggccgc
     1261 ggtgggcgtg gacgatctcc ttggagatgt acaggccgag cccgagcccg cgggtgcgct
     1321 cgcagcggtg gtggatgccg cggaacggct cgaacagggt ggccagcacg tcgtcggcga
     1381 tcgcgccgcg attggcgatc cggatcgcga cgcgctgcgg atcgctgccg tcgagctgga
     1441 cctggaccgg cgcgccgggc tcgccgtgcg ccagcgcgtt gcccacgacg ttggacagca
     1501 gctgggtgag ccggtcgccg tcccaccggc cgtgcagcgc gccggcggcg gccagatcga
     1561 cctgcgcgcc ggggtggagc tcgatgagct cgttgagcac ggccccgaac agctcggcga
     1621 gatcgagcgg ccgcggcgcg agctcgaggc cggtgacccg gacccgggcg agatcgagca
     1681 gctgcgcgat catccccgac atccgctcgg cggcgctcac gatccgggcg gcggggcggc
     1741 cgatctcggg ggcggtcgcc cggcgctgca acagctgggc ggcggtcgcg atcgtgttga
     1801 gcgggttgcg caggtcgtgg ccgagcatcg cggtgaactt ctcgttgtac tcgatggtgc
     1861 gctccagctc gacgcgcgcg cgctgctcgc gggtgacgtc gaccacgacc acgccgacct
     1921 ggctgacctc gcccgcgggg ccgcgcaccg ggtagtaggt gatcagccag tggcg
//
LOCUS       DASVOR010000736         2221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_123885,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000736 DASVOR010000000
VERSION     DASVOR010000736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123885"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="VF516_14545"
     CDS             <1..513
                     /locus_tag="VF516_14545"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX8108947.1"
                     /translation="GPRAVFDEVEPYLAALGQGASYVGEGELSRMVKICHNVFLGVVI
                     QSLAEITVLAQKAGVPRHAFLDFMNKSVLGSVFSRYKTPALVNLDFAVTFTPHLLRKD
                     LDLGLDAGRALDVPMPLASQVRDLVQALIGNGHADKDFASLILMQARASGIELAPENV
                     PVDDGLSGAS"
     gene            510..1550
                     /locus_tag="VF516_14550"
     CDS             510..1550
                     /locus_tag="VF516_14550"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8108948.1"
                     /translation="MTALATLAALVGLGIVLAAPGAAAQPAAKTPPAPQPVRVIAFDG
                     GHNLAIWAAQRQGFFEDNGVRVILSFTPSSTAAVTGMFDDKFDIAALAIDNVIAYQEG
                     QGEAKIPDHPDLFAFLAVDDGLLGLVAAPAIHRIADLKGKTLSVDAMTTGFAFVLREL
                     LLRAGLQESDVRIVSAGGTPNRYRELVAGKHDATLLRTPFELLARDRGCHVVATADGI
                     GPYLGTVAAARRSWASHNEAALVGFIRGYRAGLAWVSDPKNREIAEAILVANARDMTP
                     ALARRAYDVLLAPHSGLSRDGALDPARIRTVLALRSKFAEPKKTLDDPARYIDPSYRD
                     KAAAAPPAPPAH"
     gene            1737..>2221
                     /locus_tag="VF516_14555"
     CDS             1737..>2221
                     /locus_tag="VF516_14555"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8108949.1"
                     /translation="MGCALDQIGTADTSSDPEADQALPAIEPVVTTGSARESLSSLVV
                     GPDAPQQWSFAVLSDLHLPNPRVATVDRTIEALIALGVRLVIVTGDHTNGSEKVDHRR
                     SRVQAWWTSVTDVLEPLHDAGISVLPIAGNHDTYLSWQREGYEQAFADLVRWAAPFKV
                     NP"
BASE COUNT          327 a          830 c          743 g          321 t
ORIGIN      
        1 gggccgcggg ccgtgttcga cgaggtcgag ccgtacctcg ccgcgctcgg ccagggcgcg
       61 agctacgtcg gcgagggcga gctgtcgcgc atggtcaaga tctgccacaa cgtgttcctc
      121 ggcgtggtga tccagtcgct cgccgagatc acggtgctcg cccagaaggc cggcgtgccg
      181 cgccacgcgt tcctcgactt catgaacaag agcgtgctcg gctcggtgtt cagccgctac
      241 aagacgcccg cgctggtcaa cctcgacttc gcggtgacgt tcacgcctca cctcctgcgc
      301 aaggacctcg acctcgggct cgacgccggc cgcgcgctcg acgtgccgat gccgctcgcg
      361 tcgcaggtcc gcgacctggt acaggcgctg atcggcaacg gccacgccga caaggacttc
      421 gcgtcgctga tcctgatgca ggcccgggcg tccgggatcg agcttgcgcc cgagaacgtc
      481 ccggtcgacg acgggctgtc gggggcgtca tgaccgcgct cgccacgctc gccgcgctcg
      541 tcgggctcgg catcgtcctc gccgcccccg gcgcggctgc ccagcccgcc gccaagacgc
      601 cgcccgcccc gcagccggtc cgcgtgatcg ccttcgacgg cggccacaac ctggcgatct
      661 gggccgccca gcgccagggc ttcttcgagg acaacggcgt ccgcgtgatc ctcagcttca
      721 cgccgagctc gaccgccgcg gtcaccggca tgttcgacga caagttcgac atcgccgcgc
      781 tcgcgatcga caacgtcatc gcgtaccagg agggccaggg tgaggccaag atccccgacc
      841 accccgatct gttcgcgttc ctggcggtcg acgacggcct gctcggcctg gtcgctgcgc
      901 ccgcgatcca ccggattgcc gacctcaagg gcaagacgct gtcggtcgac gcgatgacca
      961 ccggctttgc gttcgtgctg cgcgagctgc tgctgcgcgc cgggctgcag gagtccgatg
     1021 tccgcatcgt gtccgccggc ggcacgccca accgataccg cgagctcgtc gccggcaagc
     1081 acgacgcgac gctgctccgc accccgttcg agctgctcgc cagggaccgc ggctgccacg
     1141 tggtggcgac cgccgacggg atcggcccgt acctcggaac ggtcgccgcg gcgcgccggt
     1201 catgggcgag ccacaacgag gccgcgctgg tcggcttcat ccgcggctat cgcgccgggc
     1261 tggcctgggt gtccgatccg aagaaccgcg agatcgccga ggcgatcctg gtcgccaacg
     1321 cgcgcgacat gacccccgcg ctcgcccgcc gcgcctacga cgtgctgctc gcgccgcaca
     1381 gcggcctgtc gcgcgacggc gcgctcgacc cggcgcggat ccggaccgtg ctcgcgctgc
     1441 gcagcaagtt cgccgagccg aagaagacgc tcgacgaccc ggcccgctac atcgacccca
     1501 gctatcgcga caaggcggcc gccgcgccgc ccgcgccgcc cgcgcactag tctacaaggc
     1561 atagcgccca ggagagcgcg ttggcgtctg gcgatcgcgc cccgcgaatt tattccatga
     1621 gcatccagga tcaggacctt cggaggtctc gcgaatagag cgggcgcgca ccgcgttgat
     1681 cctccatgcg ctcggccgca gtgatcgttc tcggagcgct ccccatcgta gcgagcgtgg
     1741 ggtgcgccct cgaccagatc ggcaccgcgg acaccagcag cgacccagaa gccgaccagg
     1801 cgctgccggc gatcgaaccg gtcgtgacga cgggctctgc gcgcgagtca ctctcgagcc
     1861 tggtcgtcgg tcctgacgcg ccgcagcagt ggagcttcgc cgtgctctcc gacctgcacc
     1921 tccccaaccc acgggtcgcc acggtcgacc ggacgatcga ggcgctgatc gcgctgggcg
     1981 tgcggctggt gatcgtcacg ggagaccaca cgaacgggtc ggagaaggtg gaccaccggc
     2041 gcagtcgggt tcaggcgtgg tggacttccg tcaccgatgt cctcgagccg ctgcacgacg
     2101 ccgggatctc ggtcctgccg atcgccggca atcacgacac gtacctgagc tggcagcgcg
     2161 agggctacga gcaagcgttc gccgacctcg tgcgctgggc agcgccgttc aaggtcaacc
     2221 c
//
LOCUS       DASVOR010000737         5299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_182708,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000737 DASVOR010000000
VERSION     DASVOR010000737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5299
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_182708"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VF516_14560"
     CDS             <1..974
                     /locus_tag="VF516_14560"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8108950.1"
                     /translation="RRLDARDGFGRVIAESAVMRGVVADARRVAVTDETVLLLGESGT
                     GKNLLARAIHNASARGAAPFIEVHAAALPETLVEGELFGRDKGAYTGATDAKPGHAEA
                     AHGGTLFLDEIGELAAPIQVKLLRFLQDRSFTRLGSTQPRRSDARIIAATNRDLSAAV
                     KAGSFREDLYYRLAVFPIVVPPLRERPDDVRATALSLLAARGLGPDRITAAALDQLAA
                     YAWPGNVRELENALGRALVLAGTEPIAPDHLPPAVVRTAKAAAPSILDELLVPEFSLD
                     ALERDLIHHALVKAGGNKAAAARLLGITRRRLYSRLDSLGEPAHDDE"
     gene            complement(1342..2262)
                     /locus_tag="VF516_14565"
     CDS             complement(1342..2262)
                     /locus_tag="VF516_14565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEX8108951.1"
                     /translation="MNRPTFADPKTDFVFKRIFGSEQHKDVLVAFLNDMLGLDEAHRI
                     VRVELLSPEQRPAVAELKLSIVDVKCTDARGVVYVVEMQVLQVEGFEKRVVYNVAKAY
                     VNQITQGDGYPNLNDVVGITICDFVLWPDSEARRLPMLTRWRMTEQHTGAKELGQIQL
                     VFLELPKYDVSRPPQTVVEKWAYFFREADNLTMVPEVLAEPPFIDALDAARTAGFTAS
                     EWDAYILAGIAIQNERGALAVAEKRGEQRGREDGLRQGREDGLRQGIEALCVALGIEL
                     TDPRRRELASLDAAGLETLLARLGQHKRWA"
     gene            complement(2596..5127)
                     /locus_tag="VF516_14570"
     CDS             complement(2596..5127)
                     /locus_tag="VF516_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108952.1"
                     /translation="MATTVIAALPALPPAAQLHPMYLPDYPASPAIVPPLAPLPHGST
                     VDQVARTGQSPPRVGLPEPRSETPGLTRVLRAFGLTRVGQLGEDGLLSAVFRAAIPDA
                     FRDSLQVLVEFFGELFTSAQPFGDRVVQLGTPAIGDLASRDHYVLFVDDLDREPLGGP
                     ATPEAAVLRSLRPLQTTAFRARVAPLAEQLAASRTPAGSVVIAILAAPQLGQGARETI
                     FALRKERNLYVVPIAASEVRRACEAGTARLLLLNRIADLHTVSDPFAVLDDFTDPTRC
                     IGFAAEVADLVKHIIAGGTLVSVAGPPGSGKTSVVAMAEYGCDAASAARRFVRLPCHE
                     LTRRDPEPLIRELRDCVDELDRSAAPRRSHGSIPPAPPGSGAFDLRTALLHLGARPQP
                     DGDPAAPPSPSPPRREQLVIVLEDADRLIRLASSAEPDAALRDRARELWHRLDQLCAS
                     GGHTVIVTSVRDFQDQVPLERPVDVARVPLRALNRGESDRLVNSLGELVGFSPTRRAL
                     ARLHRESGGNVYALRLLCSTVIRTMRERPGYSPLARLEVVPGMVDAAAAHIAATGSSF
                     RAHVSVWLDDIEKLVLQHVARERPRSPRQLRRALEASADAAQIVQALDGLELMGLVES
                     RRGRHRVRIPLFERWIDTHLDPPPRQRDAIQHRRVSKIAIGCTVALLLAGAYEAWLRS
                     TRSAEAQPLGDCTFELDHPDRAGTDEAFDLLVYQTCKAAAPHQLALQPVRSALRISAA
                     ASDCTPAAASCTAMFKATAGQQAHDAYRVQLVVDAQPLLASAIAKDRFASMRSIGEKT
                     VPTIAWVLPLISVVVAFHKDLRRSLRSIAQLLGRRRTPHTEPAPP"
BASE COUNT          727 a         1908 c         1977 g          687 t
ORIGIN      
        1 cccgccggct cgacgcccgc gacggctttg gccgcgtcat cgccgagtcc gcggtgatgc
       61 gcggcgtggt cgccgacgcc cgccgcgtcg ccgtcaccga cgagaccgtg ctgctgctcg
      121 gcgagagcgg caccggcaag aacctgctgg cccgcgcgat ccacaacgcg agcgcgcgcg
      181 gcgcggcgcc gttcatcgag gtccacgccg ccgcgctgcc cgagaccctg gtcgagggcg
      241 agctgttcgg ccgcgacaag ggcgcctaca ccggcgcgac cgacgccaag ccgggccacg
      301 ccgaggccgc gcacggcggc acgctgttcc tcgacgagat cggcgagctc gccgcgccga
      361 tccaggtcaa gctgctgcgc ttcctccagg accgcagctt cacccggctg ggctcgaccc
      421 agccccgccg gtccgacgcc cggatcatcg ccgcgaccaa ccgcgacctg tccgccgcgg
      481 tcaaggccgg cagcttccgc gaggacctct attaccggct cgccgtgttc ccgatcgtcg
      541 tcccgccgct gcgcgagcgc cccgacgacg tccgcgccac cgcgctcagc ctgctcgccg
      601 cgcgcggtct gggccccgac cggatcaccg ccgccgcgct cgatcagctc gcggcgtacg
      661 cctggcccgg caatgtccgc gagctcgaga acgcgctcgg ccgcgcgctc gtcctcgccg
      721 gcaccgagcc gatcgccccg gaccacctgc cgcccgccgt ggtccgcacc gccaaggccg
      781 ccgcgccctc gatcctcgac gagctgctcg ttcccgagtt cagcctcgac gcgctggagc
      841 gcgacctgat ccaccacgcg ctggtcaagg ccggcggcaa caaggccgcc gccgcccgcc
      901 tgctcggcat cacgcgccgc cgcctgtact cgcggctcga cagcctcggc gagccggcgc
      961 acgacgacga gtgagcgagc ggagcggctg cgccgacgag ccagccatcg gcgccggtat
     1021 cggaccgccg tcagccgcgc gtgctcgggc accgcgccgg ctcaccgatc cacggcctgt
     1081 agccccggcg gtccagcagg cgcacgaggt cgccccggcc atcccacacc cccaccgagg
     1141 ccgccatccc caccgcgacc atccgcaccg gggcgcctcg tgtcgccgtg atccgaccct
     1201 ggtcggccgc gcgccgcaga ccccggcgat tcacgagaag atcacgagcc gcagcggcat
     1261 gctgcgcatc gtgtgacgag ccagtgacgg ctgcgtcgcg ccgagccgaa gatcgcgcgg
     1321 cattcgacca agggagccct gtcacgccca gcgcttgtgc tgtccgagcc gcgccagcag
     1381 cgtctccagc cccgcggcgt ccaggctcgc gagctcgcgc cgccgcgggt cggtgagctc
     1441 gatgcccagg gcgacacaga gggcctcgat gccctgtcgc aggccgtctt cgcggccctg
     1501 ccgcaggccg tcttcgcggc cgcgctgctc gccccgcttc tcggccacgg ccagtgcgcc
     1561 gcgctcgttc tggatcgcga tcccggcgag gatgtaggcg tcccattcgc tcgcagtgaa
     1621 cccggcggtc cgggcggcat cgagcgcgtc gatgaacgga ggctcggcga gcacctccgg
     1681 caccatcgtc aggttgtcgg cctcgcggaa gaagtaggcc cacttctcga ccaccgtctg
     1741 cggcggccgg ctcacgtcgt acttgggcag ctccaggaac acgagctgga tctgaccgag
     1801 ctccttcgca ccagtgtgct gttcggtcat tcgccatcgg gtcagcatcg gcagccggcg
     1861 cgcctcgcta tcgggccaca gcacgaagtc gcagatcgtg atcccgacga cgtcgttgag
     1921 attcgggtac ccgtcgccct gggtgatctg gttcacatag gccttggcaa cgttgtagac
     1981 cacgcgcttc tcgaagccct cgacctggag cacctgcatc tcgacgacgt agaccacccc
     2041 gcgtgcgtcg gtgcacttga cgtcgacgat cgataacttg agctcggcga ccgccgggcg
     2101 ctgctcgggc gacagcagct cgacgcgcac gatgcgatgc gcctcgtcga ggccgagcat
     2161 gtcgttgagg aacgcgacca ggacgtcctt gtgctgctcg gagccgaaga tccgcttgaa
     2221 cacgaagtcg gtcttggggt ccgcgaaggt cgggcgattc atgctgcttg ctcggcgggc
     2281 ggctgtgcgt tcggcagaat accgcatcca cctccatcgg catcatcgcg gcgaaaaata
     2341 ggcgagcgga tccagcgtat tacgcagcat cggcgcacgt agggcgccga cgctggattt
     2401 ctcgtcaggt tcgaggccgc catggatact ggatgacgag gcacatggag ctcgagcagt
     2461 cgccgagatc gttcgccggc atcaggccgg ggtgtgcgcc gtggcctacg gcgtgaccgg
     2521 cgatcgcgcg ctccgcgagg acatcgcccc ggagacggtc ctcgcggcgt ggcgcggtgc
     2581 gccggccctg cgcgatcacg gcggcgccgg ctcggtgtgc ggcgtgcggc ggcggccgag
     2641 cagctgggcg atcgagcgca gcgatcggcg aaggtccttg tggaacgcga cgaccacgct
     2701 gatcagcggc agcacccagg cgatcgtggg aacggtcttc tcgccgatcg accgcatcga
     2761 ggcgaaccgg tccttggcga tcgccgacgc cagcagcggc tgggcgtcga cgacgagctg
     2821 gacgcggtac gcgtcgtggg cctgctggcc cgcggtggcc ttgaacatcg cggtgcacga
     2881 ggcggcggcg ggggtgcagt cggaggccgc cgcggagatc cgcagggccg agcgcacggg
     2941 ctggagcgcg agctggtgcg gcgccgcggc cttgcaggtc tggtagacga gcaggtcgaa
     3001 ggcctcgtcg gtcccggcgc gatcggggtg gtcgagctcg aaggtgcagt cgccgagcgg
     3061 ctgggcctcg gcgctgcgcg tgctgcgcag ccacgcctcg tacgcgccgg ccagcagcag
     3121 cgcgacggtg cagccgatcg cgatcttgga gacccggcgg tgctggatcg cgtcgcgctg
     3181 gcgcggcggc ggatcgaggt gggtgtcgat ccagcgctcg aacagcggga tgcgtacgcg
     3241 gtggcggccg cgccgggact cgacgaggcc catcagctcg agcccgtcga gcgcctggac
     3301 gatctgcgca gcgtcggcgc tggcctccag cgcgcggcgg agctggcgcg gcgatcgcgg
     3361 gcgctcgcgc gcgacgtgct gcaggacgag cttctcgatg tcgtcgagcc agaccgagac
     3421 gtgggcgcga aagctggacc cggtggcggc gatgtgcgcg gcggcggcgt cgaccatacc
     3481 gggcacgacc tcgagccggg cgagcggcga gtagcccggg cgctcgcgca tcgtgcggat
     3541 cacggtgctg cacaagaggc gcagcgcgta gacgttgccg ccggactcgc ggtgcaggcg
     3601 ggcgagcgcg cgccgggtcg ggctgaagcc gaccagctcg ccgagcgagt tgaccagccg
     3661 atcggactcg ccccggttga gcgcgcgcag cggcacgcgc gcgacgtcga ccgggcgctc
     3721 gagcgggacc tggtcctgga agtcccggac gctggtcacg atgacggtgt ggccgccgga
     3781 cgcgcacagc tggtcgaggc ggtgccacag ctcgcgcgcg cggtcgcgca gcgcggcgtc
     3841 gggctcggcg ctcgacgcca ggcggatcag gcggtcggcg tcctccagga cgatgaccag
     3901 ctgctcgcgc cgcggcgggc tcggcgaggg cggcgccgcg ggatcgccgt ccggctgcgg
     3961 tcgggcgccc aggtgcagga gcgccgtgcg cagatcgaac gcaccggagc ccgggggggc
     4021 cggagggatc gagccgtgcg accgccgggg agccgcgctg cggtccagct cgtccacgca
     4081 gtcccggagc tcgcggatca ggggctcggg atcgcggcgg gtgagctcgt ggcacggcag
     4141 gcggacgaac cggcgcgcgg cgctggccgc atcgcagccg tactccgcca tcgccaccac
     4201 gctggtcttg cccgacccgg gcggtccggc gacgctcacg agggtgccgc cggcgatgat
     4261 gtgcttgacc aggtccgcga cctcggcggc gaagccgatg cagcgcgtcg ggtccgtgaa
     4321 gtcatcgagg accgcgaacg ggtcgctgac ggtgtggaga tcggcgatcc ggttgagcag
     4381 gagcagccgg gcggtgccgg cctcgcatgc gcggcggacc tccgacgctg cgatcggcac
     4441 gacgtagagg ttgcgctcct tgcgcagcgc gaagatggtc tcgcgcgcgc cctggccgag
     4501 ctgcggggcg gccaggatcg cgatcaccac cgagcccgct ggggtccggg aagcggccag
     4561 ctgctcggcg agcggcgcga cgcgcgcgcg gaacgcggtg gtctggagcg ggcgcaggct
     4621 cctcaggacc gcggcctccg gcgtcgccgg cccgccgagg ggttcgcgat cgaggtcgtc
     4681 gacgaacagg acgtaatggt cgcgggacgc gagatcgccg atcgccgggg tcccgagctg
     4741 gacgacgcgg tcaccgaacg gctgcgcgct ggtgaacagc tcgccgaaga actcgaccag
     4801 gacctgcagc gagtcgcgga acgcgtcggg gatcgcggcg cggaacaccg cggagagcag
     4861 gccgtcctcc ccgagctgcc cgacgcgcgt caggccgaac gcccgcagga cccgcgtgag
     4921 ccccggagtc tcggaccttg gctccgggag ccccaccctc ggcgggctct ggccggtccg
     4981 cgccacctgg tcgacggtcg agccgtgcgg gagtggcgcc agcgggggca cgatcgccgg
     5041 cgaggccgga tagtccggca ggtacatcgg gtggagctgc gcggccggag gcagcgccgg
     5101 gagcgccgcg atgacggtgg tcgccatgct ctgcggcgtc ggggtccggg actcgccgta
     5161 ccggatctgc gtcgccggcg tcccctcggc cgggagcgcg accacggcgg gcagcggtgc
     5221 cggccggttc gccggcggaa cgctccccgg ctcgacagcc gggatcgaca gctccggcgg
     5281 tgtgccggcg gggagctca
//
LOCUS       DASVOR010000738         6358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_95275,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000738 DASVOR010000000
VERSION     DASVOR010000738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95275"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VF516_14575"
     CDS             complement(<1..739)
                     /locus_tag="VF516_14575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108953.1"
                     /translation="MRFRCALIATCAAGAVAAPAVRADDAPAARTENPRDVFGLPPAQ
                     VKAENPRDVFNLPRAPAAPSCDDGLDFGCAMATDPLDPATPYALSTWLPRDYLRRLPV
                     ADATQDLVASYALGAFRDEAGVVIGGATGLENRWTIDGAPADGLRTGAVDTLVPIQFL
                     DGLWISAGGFSARDRVSTGGMIDARLRRGTAHHEIEVDGWTGLSRAPSVGPSASGSFT
                     VRKLTADPGPFATASVVATGPIEPLSPL"
     gene            complement(846..1199)
                     /locus_tag="VF516_14580"
     CDS             complement(846..1199)
                     /locus_tag="VF516_14580"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8108954.1"
                     /translation="MAKGAARVVQHRLGARMQALRADRELTQEAVADRIGISQKYLSE
                     LERGQKSPSWETLVAIAHKGFDIKLASLMFGIDEDIGAEAKHINDVLAGRPKEARYDV
                     LRAVELILRAGEGSK"
     gene            complement(1538..1990)
                     /gene="trxA"
                     /locus_tag="VF516_14585"
     CDS             complement(1538..1990)
                     /gene="trxA"
                     /locus_tag="VF516_14585"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEX8108955.1"
                     /translation="MATVEITADNFQQTIEKPGIVLLDFWAQWCGPCRAFAPVFERAS
                     ETHQDITFGKIDTDAQQELAGAFEITAIPTLMLFRDGVPLFRHSGTLPPAAMEQLVAQ
                     ARALDMDQVRAEIAAHEHEHGPGCNHDHGEPDDDEPDAGEPDAGDREH"
     gene            2140..2718
                     /locus_tag="VF516_14590"
     CDS             2140..2718
                     /locus_tag="VF516_14590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8108956.1"
                     /translation="MSSPISPVVVGYDFSQSGRAALHRAVTLAGRAPFHVLHVVCAVD
                     PREAIPALPSYNGVDYMYAARVQEALAAETQHELDRADVQGSVHFFVHARIGKPAPEI
                     LALAEEVGADLIIVGSKRLTPLERLILGSTSEQVMRDAGCTVEIARAKQYPDVVLLPV
                     EPVDPVHTYVPPHRYTYEDHRVNLRPAEWPLY"
     gene            2771..3547
                     /locus_tag="VF516_14595"
     CDS             2771..3547
                     /locus_tag="VF516_14595"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX8108957.1"
                     /translation="MSEKPRLPGSPRRQAAIALIGSVAITLAMFLIPRPEQTWWLLAP
                     FRWLHIYVHEFGHGIAAVLVGGQFDQFEMWTYSGVATIGGVSGSFASAFVSAGGLCGP
                     AVVGGLFLATGRRPGLARVALAAFGAFMAVSLVLWTRTPFGWGFGAVVAALSLLIAIG
                     ARPAHAQAVLVFLGVQLALSVYTGGGYLFTQYVTIQNGQHTPSDTQQMSQALFLPYWF
                     WGIVCAAFSALVLLGGLWLYVRPGRTAPSVRTGGKARLAA"
     gene            complement(3605..3976)
                     /locus_tag="VF516_14600"
     CDS             complement(3605..3976)
                     /locus_tag="VF516_14600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108958.1"
                     /translation="MAKAKAKKSKTKTKTKAAKAAPAKKSAKPAAKAKPAAKAKAKPA
                     AKKPAAKKPAAKKPAAKPAKPAKPAPPPEPQMDEHEEHEEHNSSSESEHWDEPAAPPV
                     PVNEPVEDEGEHDDEYEDDMS"
     gene            complement(3988..4806)
                     /locus_tag="VF516_14605"
     CDS             complement(3988..4806)
                     /locus_tag="VF516_14605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8108959.1"
                     /translation="MSLRGKTLFITGASRGIGLAIGLRAARDGAHVAIAAKTTAPNPR
                     LPGTIYTAAEEINGAGGKALALVCDIRFEDQVTAAVAATVEAFGGIDILVNNASAISL
                     TGTEHTPMKRFDLMHQINTRGTFLCSQACLPHLRKAENPHILNISPPLNMEERWFAPH
                     VAYTMAKFGMSMCVLGMAGELRRDGIAVNALWPRTAIATAAVQNLLGGDVAVRGSRKP
                     SIMGDAAHAILTKPSREFTGRFCIDDDVLAAEGVTDFRAYAMDPNAQLLPDYFI"
     gene            complement(5448..>6358)
                     /locus_tag="VF516_14610"
     CDS             complement(5448..>6358)
                     /locus_tag="VF516_14610"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8108960.1"
                     /translation="VVAEAVRYAAAAGRPVDRDALEASVGRRLSLRLGTYGTVVHRKA
                     RFAELVLPDDVIDTLHDMIAMVRERAQILERWGYQRHLGISRGVAGLFSGEPGTGKTM
                     AASVVASELGLELVRIDLSAVVSKYVGETEKNLGRIFDEAQDAHAMLLFDEADSLFGK
                     RTELKTAQDRFANLEVNYILQRMESFDGVSVLTTNAESAIDPALQRRLNFRIRFPEPE
                     PEERARLWRQLLPPQAGLHDRVDFFALAERFEMTGGYIKNAVVRAAVIAARAGRGLTA
                     EDLWTGAHQEYVEMGKVMPQLPRGTL"
BASE COUNT          863 a         2253 c         2206 g         1036 t
ORIGIN      
        1 ccagcggcga cagcggctcg atcggcccgg tcgccaccac cgacgcggtc gcgaacggcc
       61 ccgggtcggc ggtcagcttg cgcaccgtga agctgcccga cgcgctcggc ccgacgctcg
      121 gcgcgcgcga cagcccggtc cagccgtcga cctcgatctc gtggtgcgcg gtgccgcgcc
      181 gcagccgggc gtcgatcatg ccgccggtgc tcacccgatc gcgcgccgag aacccgccgg
      241 cgctgatcca cagaccgtcg aggaactgga tcggcaccag ggtgtcgacc gcgccggtgc
      301 gcaagccgtc ggccggcgcg ccgtcgatcg tccagcggtt ctcgaggccg gtcgccccgc
      361 cgatcaccac gccggcctcg tcgcggaacg cgccgagcgc gtagctggcc accaggtcct
      421 gggtcgcgtc ggcgaccggc agccggcgca ggtagtcgcg cggcagccag gtcgacagcg
      481 cgtacggcgt ggccgggtcg agcgggtcgg tcgccatcgc gcagccgaag tccaggccgt
      541 catcgcacga cggcgcggcc ggggcccggg gcaagttgaa cacgtcgcgc ggattctcgg
      601 ccttgacctg ggccgggggc aggccgaaca catcgcgcgg attctcggtc cgggccgcgg
      661 gcgcatcgtc ggcgcggacg gcaggtgcgg cgaccgcgcc ggctgcgcag gtcgcgatca
      721 gcgcgcaccg gaaccgcacg cttgaccgta acacgacgca cgcggttcac cgcgacgccg
      781 gccgtcgatg tgtgacgggc tcggcgcgcc cgccccctct ggtaggagcg agcgccggag
      841 cgcgcctact tggagccttc gccggcgcgc aggatcagct cgaccgcgcg cagcacgtcg
      901 tagcgggcct ccttgggccg gccggcgagc acgtcattga tgtgcttggc ctcggcgccg
      961 atgtcctcat cgatgccgaa catcagcgac gcgagcttga tgtcgaagcc cttgtgggcg
     1021 atcgcgacca gcgtctccca gctcggtgac ttctgcccgc gctccagctc actcaggtac
     1081 ttctgcgaga tgccgattcg atcggcgacc gcttcctgcg tgagctcgcg atcggcgcga
     1141 agcgcctgca tgcgcgcccc caggcgatgc tgaacaactc gggcggcacc tttggccatc
     1201 gatcgcgagc gtaggtttgc tcgctccatc tctgtaacac gctggtagct ctgctatggg
     1261 ttatctatac ccacccatag cagtgaagcc cacagtttct ggaagcatcc gatctctccg
     1321 agatcctctt gcttcaacgc cacacgcatc gccgcccccg atcgacgcgc gcggtgcggc
     1381 cgaccatcgt gccgaaatcc gcaacgattc cgcatggatt gcaggaccgg cggcgcggcc
     1441 gcgccacgcg gacccagctt ctggctcatc accgtcccgg atttgcgttc acccgctgcc
     1501 ccgcggtgca gggtccccga gggacccatg cgccggatca gtgctcgcgg tcgccggcgt
     1561 cgggctcgcc ggcgtcgggc tcgtcgtcgt cgggctcgcc gtgatcgtgg ttgcagccgg
     1621 gcccgtgctc gtgctcgtgc gccgcgatct cggcgcggac ctggtccatg tcgagcgcgc
     1681 gggcctgggc gaccagctgc tccatcgccg cgggcggcag cgtcccggaa tgccggaaca
     1741 gcggcacgcc atcgcggaac agcatcagcg tcgggatcgc cgtgatctcg aacgctccgg
     1801 cgagctcctg ctgagcgtcg gtgtcgatct tgccgaacgt gatgtcctgg tgagtctcgg
     1861 acgcgcgctc gaacaccggc gcgaacgccc ggcacgggcc gcaccactgg gcccagaagt
     1921 cgagcaggac gatcccaggc ttctcgatcg tctgctggaa gttgtccgcc gtgatctcga
     1981 ctgttgccat cgcgcgacca tggcctgtcg cgcgagacac cgcaagagcg ccgatcgggt
     2041 tgcgagcgca cgcgcgcggc gcgcggccgc cgcccgctgc accgcggccc ttccctcctg
     2101 gacaagaacc gtggcacgag cgttgctgcg ctcgcgacca tgagctcgcc gatcagtccg
     2161 gtcgtcgtcg gctacgactt cagccagagc ggccgcgccg ctctccaccg cgcggtaacg
     2221 ctcgccgggc gtgcgccgtt tcatgtcctg cacgtcgtgt gcgccgtgga tccccgggaa
     2281 gcaatccctg ccctgccctc gtacaacggt gtcgactaca tgtacgcggc gcgtgtccag
     2341 gaggccctcg ccgccgagac ccagcacgag ctcgaccgcg ccgacgtcca gggcagcgtg
     2401 cacttcttcg tccacgcgcg gatcggcaag cccgcgccgg agatcctggc gctcgccgag
     2461 gaggtcggcg cggacctcat catcgtcggc agcaagcggc tgaccccgct ggagcggctg
     2521 atcctcggct cgacctccga gcaggtgatg cgcgacgccg gctgcaccgt cgagatcgcc
     2581 cgggccaagc agtatccgga cgtggtgctg ctcccggtcg agccggtcga tcccgttcac
     2641 acctacgtgc cgccgcaccg ctacacctac gaggatcacc gcgtgaacct gcgcccggcg
     2701 gagtggcctt tgtactgacg attcgcgtgg gtggcatggc gccgggcgca cagcggtcta
     2761 catttacagg atgtcggaga aaccgcgctt gccagggtcg cctcggcgcc aggcggcgat
     2821 cgcgctgatc ggcagcgtcg cgatcacgct cgcgatgttt ctgatccccc gtccggagca
     2881 gacctggtgg ctgctcgccc cgtttcgctg gctgcatatc tacgtccacg agttcggcca
     2941 cggcatcgcc gctgtgctgg tgggcggaca gttcgaccag ttcgagatgt ggacgtactc
     3001 gggagtcgcc accatcggcg gcgtctcggg gagttttgcc tcggcgttcg tgagcgccgg
     3061 cggactgtgc ggcccggccg tggtcggcgg gctgttcctg gcgaccggcc gccgccccgg
     3121 gctcgcgcgg gtcgcgctcg ctgcgttcgg cgcgttcatg gcggtgtcgc tcgtactgtg
     3181 gacccgcacc ccgttcggct gggggttcgg cgccgtggtc gccgcgctga gcctgctgat
     3241 cgcgatcggc gcgcgtccgg cgcacgccca ggccgtgctc gtgttcctcg gcgtgcagct
     3301 cgcgctgagc gtctacaccg gcggcggcta cctgttcacc cagtacgtga cgatccagaa
     3361 cggccagcac accccgtcgg acacccagca gatgtcccag gcgctgttcc tgccgtactg
     3421 gttctggggg atcgtatgcg cggcgttctc ggccctggtg ctgctcggcg ggctgtggct
     3481 gtacgtccgg cccggccgca cggcgcccag cgtgcgaacc ggcggcaaag cgcgactggc
     3541 cgcctagcgc ggctcacctc cgttgccatc gtcgccatcg tcgcagtccg gccggcggcg
     3601 ccggctacga catgtcgtct tcgtactcgt cgtcgtgctc gccttcgtcc tcgaccggct
     3661 cgttgaccgg caccggaggc gcggcgggct cgtcccagtg ctcggactcg gaggagctgt
     3721 tgtgctcctc gtgctcctcg tgctcgtcca tctggggctc ggggggcggg gccggcttgg
     3781 cgggcttggc gggcttggcc gcgggcttct tggccgcggg cttcttggcc gcgggcttct
     3841 tggccgcggg cttggccttg gccttcgcgg cgggcttggc tttcgcggca ggcttcgccg
     3901 acttcttcgc cggcgccgcc ttggcggcct tcgtcttcgt cttggtcttc gacttctttg
     3961 ctttggcctt ggccattgtg gcgctcccta gatgaagtaa tcgggcaaga gctgagcgtt
     4021 gggatccatc gcgtacgcgc ggaagtcggt cacgccttcc gcggcgagta cgtcgtcgtc
     4081 gatgcagaac cggccggtga actcgcgcga tggcttggtc aggatcgcgt gggccgcgtc
     4141 gcccatgatc gacggcttgc gcgagccccg gaccgcgacg tccccgccga gcaggttctg
     4201 gaccgcggcg gtggcgatcg cggtgcgcgg ccacagcgcg ttgaccgcga tgccgtcgcg
     4261 gcggagctcg ccggccatgc cgagcacgca catgctcatc ccgaacttcg ccatcgtgta
     4321 cgcgacgtgc ggcgcgaacc accgctcctc catgttgagc ggaggcgaga tgttgaggat
     4381 gtgcgggttc tccgccttgc gcaggtgcgg caggcaggcc tgcgagcaca ggaacgtgcc
     4441 gcgcgtgttg atctggtgca tcagatcgaa ccgcttcatc ggcgtgtgct cggtgccggt
     4501 caggctgatc gcgctcgcgt tgttgaccag gatgtcgatg ccgccgaacg cctcgacggt
     4561 cgccgccacc gcggcggtca cctgatcttc gaaccggatg tcacagacca gcgcgagcgc
     4621 cttgccgccc gcgccgttga tctcctccgc cgcggtgtag atcgtgcccg gcagcctggg
     4681 gttgggcgct gtggtcttcg cggcgatcgc gacatgggcg ccatcgcgcg cggcgcggag
     4741 cccgatcgcc agcccgatgc cgcgactggc accggtgatg aacagcgtct ttccccgcag
     4801 cgacatgaga atacgctaga ccagaatgcg tcgaggttca tcagaactgg tcggggttca
     4861 tcgcgtcgcg ggcagatccg gatctccccg gtcgatctat ctcgcacgcc gcttgcgccc
     4921 cggcgatgcc ggtgaccgag ccgatcccgg aacaccaggc cccccgagca accccgacgc
     4981 cggggcgtcg tggctgcgaa gacacacccg gtgaggagcc cgatcctcac tccgagcagc
     5041 gaaattgtcg tcgggaacac cggaccgtcg ggtcggcggt gcgcggcggc aatcacgacg
     5101 gcaccccgga gctccgccgg ctgcaccgcg gatccggcgt gcgcaaacgc tcgctcgctc
     5161 gcgatcaccg aacgagattt ttctagtact atcaaacaga tatgacaagg tgggcagccg
     5221 acagcagggc ccggcaatcc acatccgagt gtggcatcgc gcggtcagcg gcgccggggc
     5281 gagcaagccc gcggcgcgag aagcgcgcaa cgatcgcgcg cgccgcgagc tccgctgcga
     5341 gcgggacccc ttcggcgcgg aggtgaccga gagttgcgcg cacgatccgc gctggagaca
     5401 tcttcggtca tggacgcctc acaggaacac cggggagatc ccgtgggtca cagggtcccc
     5461 cgcggcagct gcggcatgac cttccccatc tccacatatt cctggtgggc gccggtccag
     5521 aggtcctcgg cggtcaggcc gcggccggcc cgggcggcga tcacggcggc gcggaccacc
     5581 gcgttcttga tgtaaccccc agtcatttca aagcgttccg ccaaggcgaa gaagtcgacc
     5641 cggtcgtgca gcccggcctg cggcggcagc agctggcgcc acagccgggc gcgctcctcg
     5701 ggctccggct cggggaaccg gatccggaag ttgagccggc gctgcagcgc cgggtcgatc
     5761 gcgctctcgg cgttggtggt caggaccgac acgccgtcga agctctccat ccgctgcagg
     5821 atgtagttga cctcgaggtt ggcgaaccgg tcctgggcgg tcttgagctc ggtccgcttg
     5881 ccgaacagcg agtcggcctc gtcgaacagg agcatcgcgt gggcgtcctg agcctcgtcg
     5941 aagatccggc cgaggttctt ctcggtctcg ccgacgtact tcgacaccac ggcggacagg
     6001 tcgatccgga ccagctcgag gccgagctcg gaggccacga cgctggccgc catggtcttg
     6061 ccggtgcccg gctcgccgga gaacagcccg gcgacgccgc gcgagatccc gaggtggcgc
     6121 tggtagcccc agcgctcgag gatctgggcg cgctcgcgga ccatcgcgat catgtcgtgc
     6181 agcgtgtcga tcacgtcgtc gggcagcacc agctcggcga accgcgcctt gcggtggacc
     6241 acggtgccgt aggtcccgag ccggagcgac agccggcggc cgaccgaggc ctcgagcgcg
     6301 tcgcgatcga ccggccggcc ggccgccgcg gcgtagcgaa ctgcctcggc gacgacat
//
LOCUS       DASVOR010000739         2812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_211744,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000739 DASVOR010000000
VERSION     DASVOR010000739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211744"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VF516_14615"
     CDS             complement(<1..300)
                     /locus_tag="VF516_14615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108961.1"
                     /translation="MPRPSRIAATMVAKLSSASTMSDTSRVTSVPVLPIAIPTWASRS
                     AGASLTPSPVIATIAPRAFQSRTLRSLSSGVARAWTRPSGGSPGRAMPSSRAITCA"
     gene            complement(328..696)
                     /locus_tag="VF516_14620"
     CDS             complement(328..696)
                     /locus_tag="VF516_14620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108962.1"
                     /translation="MLRDANDRNSWTTTSTATARLRARVRPPSSAHANTPNVTAVCSA
                     PVTASSRHSARPRIGSAGGRGGRRMTSASGGSTTSASAGRPSVTRLIHNSWSGNSGSG
                     SPASAAISITASSAVLPDSR"
     gene            complement(791..1798)
                     /locus_tag="VF516_14625"
     CDS             complement(791..1798)
                     /locus_tag="VF516_14625"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEX8108963.1"
                     /translation="MTRACITWFAALCVSACSSTERSSSDEQDLWELQGTQLQGVQML
                     GTQAQGMTLVGFQFAGATLNGAALSNVHIEKGELVATQNQVELRGLALKDAHLIAQLH
                     NVHVNPPTTLSVEYWISDIVAESASYDPTNTGHTFLYTLKQNVDNTGDWELACPVDLD
                     SDHVAIPTTAIWDEHGNRIESNTLFTFGCTSGAVGKCYRWGYRPWLTGYGDVVSAHWA
                     CTRLARSDFCGNGTSTTHEGTEINMWDNLPSPGPIQARGTTPPGMLFEAGWNTSGAVC
                     LSHQRWLVDGTLVALQCPLRLLPPLLGVLNPTVCDTVSQVLGLNADALIFNESYILDL
                     Y"
     gene            complement(1859..>2812)
                     /locus_tag="VF516_14630"
     CDS             complement(1859..>2812)
                     /locus_tag="VF516_14630"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8108964.1"
                     /translation="PSLRLPAGSLVLAFEAPGHAPARLPVLLAHGETLAVRISLPAAA
                     AVPPGMIYVPPGRFLFGGAEDAERQVFKAAPLHEVTTPGYLIGRYEVTFAQWIEYLDD
                     LAPEERRRRTPRSDNVVTTATALTLTEIGGGRWRLRLTPTTRTYTAETGQRLHYERRA
                     KRADQDWMRFPVSGVSYEDAIAFASWLDRTRRIPGARLCNEYEWERAARGADARASSD
                     GDTFAPDDANVDVTYGREPLAFGPDEVGSHPGSRSPVGADDMAGNVWEWTRSVEHLDE
                     PVLRGGSWYQGVLSARSMNREHGEPTLRGTLVGLRLCATPP"
BASE COUNT          387 a          997 c          992 g          436 t
ORIGIN      
        1 cgcgcaggtg atcgcgcgcg agctcggcat cgcgcggccg ggcgagccgc ccgacggccg
       61 ggtccatgcc cgggcgacgc ccgaggacaa gctgcgcagc gtgcgcgact ggaaggcgcg
      121 cggcgcgatc gtcgcgatga ctggcgacgg ggtcaacgac gcgccggcgc tgcgcgaggc
      181 ccacgtcggg atcgcgatgg gcaggaccgg caccgaggtg acccgcgagg tgtccgacat
      241 cgtgctcgcc gacgacaact tcgcgaccat cgtcgccgcg atccgcgagg gccgcggcat
      301 cttcgacaac atccgcaagg cgctggtcta cctgctgtcg ggcaacaccg ccgagctcgc
      361 cgtgatgctg atcgccgcgc tcgccgggct gccgctgccg ctgttgccgc tccagctgtt
      421 gtggatcaac ctggtcaccg acggcctgcc ggcgctcgcg ctggtcgtcg atccgcccga
      481 ggccgacgtc atgcgccggc cgccccggcc gccggccgaa ccgatcctcg gccgcgccga
      541 gtggcgcgag ctcgcggtga ccggcgcgct gcagaccgcg gtgacgttcg gcgtgttcgc
      601 gtgggcgctg gacggcggcc tgacccgcgc gcgcagcctg gcggtcgccg tgctggtggt
      661 cgtccagctg ttccggtcgt tcgcgtcgcg cagcacgacc cgctcgacgc cgcgctgtgc
      721 ctggcgctcg ggctggtgcc ggtcaccgtg gtcgagctcg ccaagctggt ccgcgccggc
      781 gccgggcgga tcagtacaga tccaggatgt aggactcgtt gaagatcagg gcgtccgcgt
      841 tcaggccgag cacctgcgag acggtgtcgc acacggtcgg gttcaggacc cccaggagcg
      901 gcgggagcag ccgaaggggg cattgcagcg cgaccagcgt cccgtcgacc agccagcgct
      961 ggtggctcag gcacaccgcg ccgctggtgt tccacccggc ctcgaacagc atccccggcg
     1021 gcgttgtccc gcgggcctgg atcggccccg gcgacggcag gttgtcccac atgttgatct
     1081 ccgtgccctc gtgcgtggtc gacgtgccat tgccgcagaa gtcggagcgc gccagccgtg
     1141 tgcaggccca gtgggcggag accacgtcgc cgtatccggt cagccacggc cggtagcccc
     1201 agcggtagca cttgccgacc gcgccgctgg tgcagccgaa cgtgaacagc gtgttcgact
     1261 cgatccggtt gccgtgctcg tcccagatcg cggtcgtcgg gatcgcgacg tgatcgctgt
     1321 cgaggtccac cggacaggcg agctcccagt cgccggtgtt gtcgacgttt tgtttgagcg
     1381 tgtagaggaa ggtgtgcccg gtgttcgtcg ggtcgtagct cgcggactcc gccacgatgt
     1441 cgctgatcca gtactcgacg gacagggtcg tcggcgggtt gacgtggacg ttgtgcagct
     1501 gggcgatcag gtgcgcgtcc ttgagcgcga gaccgcgcag ctcgacctgg ttctgcgtgg
     1561 cgaccagctc gcccttctcg atgtgcacgt tgctcagcgc cgcaccgttc agcgtggcgc
     1621 cggcgaactg gaagcccacg agcgtcatgc cctgggcctg ggtgcccagc atctgcacgc
     1681 cctgcagctg ggtgccctgc agctcccaga ggtcctgttc atcggagctg gaccgctccg
     1741 tggatgaaca cgcagacacg cacagcgccg cgaaccatgt gatgcaagct ctcgtcatga
     1801 aatccccttg tccatcgtcg ttgttgtcgg aatcgtgaca ttctgtatgt gcacgcgatc
     1861 agggtggcgt cgcgcacagg cgcaatccca ccagggtgcc acgcagggtg ggctcgccgt
     1921 gctcacggtt catgctgcga gcgctgagca cgccctggta ccagctgccg ccgcgcagca
     1981 ccggctcatc cagatgctcg accgaacgcg tccactccca cacgttgccg gccatgtcgt
     2041 cggcgccgac cggactgcgc gagccggggt gcgagccgac ctcgtccgga ccgaacgcga
     2101 ggggctctcg gccgtacgtc acatcgacgt tcgcatcatc cggcgcaaag gtgtcgccgt
     2161 cggagctcgc gcgagcatcg gccccgcggg cggcgcgctc ccactcgtat tcgttgcaca
     2221 gccgcgcacc cgggatgcga cgcgtgcgat cgagccagct cgcgaacgcg atggcatcct
     2281 cgtacgacac tccggacacc ggaaacctca tccagtcctg atccgcgcgc ttcgccctgc
     2341 gctcgtagtg cagccgctgc ccggtctcgg cggtgtaggt ccgcgtcgtc ggcgtcaagc
     2401 ggagcctcca ccgcccgcct ccgatctcgg tgagcgtgag cgcggtcgcc gtggtcacca
     2461 cgttgtccga cctcggggtg cgccggcggc gctcctcggg cgcgaggtcg tcgagatact
     2521 cgatccactg ggcgaaggtc acctcgtatc tgccgatgag gtagccgggg gtcgtgacct
     2581 cgtggagcgg cgccgccttg aacacctggc gctccgcatc ctccgcgccg ccgaacagga
     2641 accggcccgg cggcacgtag atcatccccg gcggtaccgc cgccgccgcg gggagcgaga
     2701 tccggaccgc cagggtctcg ccgtgcgcca ggagcaccgg gagccgcgcc ggcgcgtgcc
     2761 cgggcgcctc gaacgcgagc accagcgagc cggccggcag gcggagcgac gg
//
LOCUS       DASVOR010000740         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_474819,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000740 DASVOR010000000
VERSION     DASVOR010000740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474819"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VF516_14635"
     CDS             complement(<1..290)
                     /locus_tag="VF516_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8108965.1"
                     /translation="MRVLVTGATGFLGEHLCRVLVERGHTVRGLARTRSAILAELGVE
                     HIRGDVLAGPELDRALDGVEAVCHLAGAVSRDPGDAQRMMRLHVDGTRRVLER"
     gene            complement(423..>1691)
                     /locus_tag="VF516_14640"
     CDS             complement(423..>1691)
                     /locus_tag="VF516_14640"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX8108966.1"
                     /translation="REHVKKVGKGLPLYKRLKVVHLWDHDLPKTASRKVRRRDVIKEL
                     ERLERAARGGAELKKLETQPGSAGAWLHDLIADVAQKKRGTVTAEARLDELGFDSLMF
                     TELAVALEAAGVELPDPAELQAVETVADVERLIARLGARTRSDKPRRDRIAREKAAED
                     KKAAADDDIAVPRPLVALGRRALRGGMKALYRNVLDTQVYGAAQVPPFGGYIVCANHA
                     SHLDTGLIKYALGEQGEALVALAAKDYFFDDPVRRMYFENFTNLVPMERHGSLRESLR
                     LAGEVLRDGYILLIFPEGTRSETGVMTDFKPSLGYLALTNKCGILPMYLAGTHEAMPK
                     GRYLPRRGERVAAHIGPFVSYREVVELAGKGSRSEQYKAVTHHIEGVIRRLAPRDQAW
                     TLGDAGTMPMAEYLALHGKASAHAEDDARE"
BASE COUNT          207 a          629 c          592 g          263 t
ORIGIN      
        1 cgctccagca cgcgccgcgt gccgtcgacg tgcagccgca tcatccgctg cgcgtcgccg
       61 ggatcgcgcg acaccgcgcc cgcgaggtgg cacaccgcct cgacgccgtc gagcgcgcgg
      121 tcgagctcgg ggccggccag cacgtcgccg cggatatgct cgacgccgag ctcggcgagg
      181 atcgccgagc gcgtacgggc caggccgcgc acggtgtggc cgcgctcgac caggacgcgg
      241 cacaggtgct cgccgaggaa tccggtcgcg ccggtgacca ggaccctcac gcgcctgcct
      301 cgtctttccg gaggggacct ttccgtcccc tctgccgcgg cggagccgcg acattcaccc
      361 ctccgcgcgt cgcgtcgcgc ggccctgcac ctgcgagctc ggtgcttctc ggttcgaaat
      421 gctcattcac gcgcgtcgtc ctccgcatgc gcgctggcct tgccgtgcag cgccaggtac
      481 tcggccatcg gcatggtgcc ggcatcgccg agcgtccacg cctgatcgcg cggggcgagc
      541 cgccggatca cgccctcgat gtggtgggtc accgccttgt actgctcgct gcgcgagccc
      601 ttgcctgcga gctccaccac ctcgcggtac gacacgaacg ggccgatgtg cgccgcgacc
      661 cgctcgccgc gccgtggcag gtagcggccc ttgggcatcg cctcgtgggt gcccgccagg
      721 tacatcggca ggatgccgca cttgttggtc agcgcgaggt agccgagcga cggcttgaag
      781 tcggtcatca cgccggtctc gctccgcgtg ccctcgggga agatcaggag gatgtagccg
      841 tcgcgcagga cctcgccggc gagcctgagc gactcgcgca gcgagccgtg gcgctccatc
      901 ggcaccaggt tggtgaagtt ctcgaagtac atccggcgca ccggatcgtc gaagaagtag
      961 tccttggccg cgagcgcgac cagcgcctca ccctgctcgc cgagcgcgta cttgatgagc
     1021 ccggtgtcga ggtggctggc gtggttggcg cacacgatgt agccgccgaa cggcgggacc
     1081 tgggccgcgc cgtagacctg ggtatcgagc acgttccggt acagcgcctt catcccgccg
     1141 cgcagcgccc ggcggccgag cgcgaccagc gggcgcggca cggcgatgtc atcgtcggcc
     1201 gccgccttct tgtcctcggc cgccttctcg cgggcgatcc ggtcgcgccg cggcttgtcg
     1261 ctgcgggtcc gggcgccgag ccgcgcgatc agccgctcga cgtcggccac agtctccacc
     1321 gcctggagct cggccggatc ggggagctcc accccggcgg cctcgagcgc gaccgcgagc
     1381 tcggtgaaca tcagcgagtc gaagccgagc tcgtcgagcc gtgcctcggc ggtcacggtg
     1441 ccgcgcttct tctgcgcgac gtcggcgatc aggtcgtgca gccacgcgcc ggcgctgccg
     1501 ggctgggtct cgagcttctt gagctcggcg ccgccccggg ccgcgcgctc caggcgctcc
     1561 agctccttga tcacgtcgcg ccgccggacc ttgcgcgacg cggtcttggg caggtcgtgg
     1621 tcccacaggt ggacgacctt gagccgcttg tacagcggca ggcccttgcc gaccttcttg
     1681 acgtgctcgc g
//
LOCUS       DASVOR010000741         1745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_240798,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000741 DASVOR010000000
VERSION     DASVOR010000741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1745
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240798"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1466)
                     /locus_tag="VF516_14645"
     CDS             complement(<1..1466)
                     /locus_tag="VF516_14645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8108967.1"
                     /translation="MQAPIQGSKAAITATVMAAALMAMIDISIVNVALSDIRASFGTP
                     IDQIGWVSTGYMMANIVVIPMTGWLQRRFGFRRYFSTSVLIFTIASALCGLSWNLPSL
                     VLFRVLQGIGGGAIIPTSQAILFARYPRAQHGMAAALFGLGASTGPLLGPTIGGYMID
                     WANWHWIFLVNVPIGLTVAFLIPRVLEEPGFTPDMTRIDATGIGLLAIGMASLQYVLE
                     EGNREGWADSTRILVLGALAAIAILTFIVHELEAPHPVVDLRGFKSRSYSAGTGLNFL
                     LGLAVFGAAYLFSLYCGAVMRYQALDIGRVFLLAGASQIFLMPAIGKIANRLDPRVML
                     VWGVGFTTLSQYVASHLYSDAAFIDLVVPNLIRSFGLAFVFIPVSVAALSDLPLAQRG
                     NATGLFNLTRELGGSLGTAWMGKVVADGITTHSSQLGEHVTSYNPIAQAQWLAIARGG
                     MDPPTMLAGRVLREAMVLSFEDGFRITMTAIGLGIVMV"
     gene            complement(1470..>1745)
                     /locus_tag="VF516_14650"
     CDS             complement(1470..>1745)
                     /locus_tag="VF516_14650"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX8108968.1"
                     /translation="VVANLKETQLADIKDGQPAEIEIDAFDGTLNGHVDSIAAGTGSR
                     FSLLPPENASGNFIKVTQRVPVRIKIDDRRGLVLRPGMSAEVVIHTR"
BASE COUNT          301 a          588 c          608 g          248 t
ORIGIN      
        1 atcaccatca cgatgccgag gccgatcgcg gtcatcgtga tccggaagcc gtcctcgaac
       61 gacagcacca tggcctcgcg cagcacccgg cccgccagca tggtcggtgg gtccatcccg
      121 ccgcgcgcga tcgcgagcca ctgggcctgg gcgatcggat tgtaggacgt gacgtgctcg
      181 ccgagctgcg acgagtgggt ggtgatgcca tcggcgacga ccttgcccat ccacgccgtg
      241 ccgagcgagc cgccgagctc ccgggtcagg ttgaacagcc cggtcgcatt gccgcgctgc
      301 gcgagcggca gatcggacag cgccgcgacg ctgaccggga tgaacacgaa cgccaggccg
      361 aacgagcgga tcaggttcgg caccaccaga tcgatgaacg cagcgtccga gtacaggtgc
      421 gacgccacgt actgcgacag cgtcgtgaac ccgacgcccc acaccagcat cacccgcggg
      481 tcgagccggt tggcgatctt gccgatcgcc ggcatcagga agatctgcga cgcgccggcg
      541 agcaggaaca cccggccgat gtcgagcgcc tggtagcgca tgaccgcgcc gcagtacagc
      601 gagaacaggt aggctgcgcc gaacaccgcg agcccgagca ggaagttgag ccccgtgccg
      661 gcggagtagc tgcggctctt gaacccgcgc aggtcgacca ccgggtgggg ggcctcgagc
      721 tcgtggacga tgaacgtgag gatcgcaatc gcggcgagcg cgccgagcac caggatccgc
      781 gtgctgtcgg cccagccctc gcggttgccc tcctcgagca cgtactgtag cgacgccatc
      841 ccgatcgcga gcaggccgat gccggtcgcg tcgatccgcg tcatgtcggg cgtgaacccg
      901 ggctcctcca gcacgcgcgg gatcaggaac gcgacggtca gaccgatcgg cacgttgacc
      961 aggaagatcc agtgccagtt cgcccagtcg atcatgtagc cgccgatcgt cgggccgagc
     1021 agcgggccgg tgctcgcgcc gagcccgaac agcgccgccg ccatgccgtg ctgggcgcgc
     1081 gggtagcgcg cgaacaggat cgcctgcgag gtcgggatga tcgcgccgcc gccgatgccc
     1141 tgcagcacgc ggaacagcac cagcgacggc aggttccacg acaggccgca cagcgcgctg
     1201 gcgatcgtga agatcaggac cgaggtcgag aagtagcgcc ggaacccgaa ccggcgctgc
     1261 agccagccgg tcatcgggat cacgacgatg ttggccatca tgtagccggt cgaaacccag
     1321 ccgatctggt cgatcggcgt gccgaagctg gcgcggatgt cgctgagcgc gacgttgacg
     1381 atcgagatgt cgatcatcgc catcagcgcc gccgccatca cggtcgccgt gatcgcggcc
     1441 ttggagcctt gaatcggggc ctgcacggct taccgggtgt ggatcacgac ctcggcgctc
     1501 atgccggggc gcagcacgag gccgcggcga tcgtcgatct tgatcctgac tggcacgcgc
     1561 tgggtgacct tgatgaagtt gccgctcgcg ttctccggcg gcagcagcga gaaccgcgag
     1621 ccggtgccgg cggcgatgct gtcgacgtgg ccgttcagcg tgccgtcgaa cgcgtcgatc
     1681 tcgatctcgg ccggctggcc gtccttgatg tcggcgagct gggtctcctt gaggttggcg
     1741 accac
//
LOCUS       DASVOR010000742         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415719,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000742 DASVOR010000000
VERSION     DASVOR010000742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415719"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF516_14655"
     CDS             complement(<1..552)
                     /locus_tag="VF516_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX8108969.1"
                     /translation="MTKAKAGGEQPVEIPDTLPILPLRNSVLFPGAIIPIDVGRRKSV
                     RLVEDAIAKERPVIGILTQKDARTEDPGSGDLYMVGCAARILKVIKLAKDNFSVILQG
                     VSRFEVAMFDGAEPFLAARVRSIPDPTSSDVELDALVMNLKDIAKRVVKLMPELPKEA
                     GALVDSVTEAGHLADLITSHLELE"
     gene            874..>1821
                     /locus_tag="VF516_14660"
     CDS             874..>1821
                     /locus_tag="VF516_14660"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8108970.1"
                     /translation="MQPANFSTILGVGPAAGRSLPGTRIGQYEVIRLLGRGGMGEVHL
                     ARDLRLGRLVAIKLLTTRQPNLGERFLAEARATAHCHHENIVVIHEVGEHDGTPYMVF
                     EYLEGQTLRQWLTDTAGPHARVPPARAVELMLPVVRALVYAHDQGIVHRDLKPENVML
                     TRGGGVKVLDFGVAKRLTAPVIAAERITEDVAPDAASSTLAGTLPYMSPEQLTLGAVD
                     HRADVWAAGIMLFELVAGAHPVLDPSAPPAALIAIADPDVAMPCATERLPEIGSLAAV
                     IDRCLIKQPEHRTASARVLLAELDGLAPGRRSVAVGDDGS"
BASE COUNT          253 a          641 c          648 g          279 t
ORIGIN      
        1 ctcgagctcg aggtggctcg tgatcagatc cgcgaggtga ccggcctcgg tgacgctgtc
       61 gaccagggcg ccggcctcct tgggcagctc cggcatcagc ttgaccacgc gcttggcgat
      121 gtccttgagg ttcatgacca gggcgtcgag ctcgacgtcc gacgatgtcg gatccggaat
      181 gctgcggacc cgggcggcga ggaacggctc ggcgccgtcg aacatcgcga cctcgaaccg
      241 cgacacgccc tgcaggatca ccgagaagtt gtccttggcg agcttgatga ccttgaggat
      301 ccgcgcggcg cacccgacca tgtacaggtc gcccgatccc gggtcctcgg tgcgcgcgtc
      361 cttctgggtc aggatgccaa tgaccgggcg ctccttggcg atcgcgtcct cgaccagccg
      421 gaccgacttg cgacggccca cgtcgatggg gatgatcgca ccggggaaca acacggagtt
      481 gcgcagcggg aggatgggga gcgtgtcggg aatctcgacc ggctgctctc cccctgcctt
      541 tgccttggtc atggactgga agctagcggc ggatctccgg gggcgtcaag ccgacctgcc
      601 tgtaatgacg ggcagtttct cgtcggcctc acgcacgtga ggccgacgag acgcgcgcca
      661 cctacaggtg cggtcccggt cgattcacgg gcgggcgggt tttccgtgaa tcgccgatca
      721 tggcgggcgc ggcggcgctt ttgcgggtcc ggagctgccg ggcggagcgg cgttgccggg
      781 gtttgacgct gggctttgca ggacccggcg ttcatggtga actgccgtcc tcgccgcgtc
      841 gggtcacgtc gcgattcacc tcgcggtttc cgcatgcagc cagccaactt ctccacgatc
      901 ctgggtgtcg gcccggccgc gggacggtcg ttgccgggca cccggatcgg ccagtacgag
      961 gtgatccggc tgctcgggcg cggcggcatg ggcgaggtgc acctggcccg cgatctccgc
     1021 ctcggccggc tggtcgcgat caagctgctg accacccggc agcccaacct cggcgagcgg
     1081 ttcctggccg aggcgcgggc caccgcgcac tgccaccacg agaatatcgt ggtgatccac
     1141 gaggtcggcg agcacgacgg cacgccgtac atggtgttcg agtacctgga gggtcagacg
     1201 ctgcggcagt ggctgaccga caccgccggc ccccacgcgc gggtcccgcc cgcccgcgcg
     1261 gtcgagctga tgctgccggt ggtgcgcgcg ctggtctacg cgcacgacca gggcatcgtc
     1321 caccgcgatc tcaagcccga gaacgtcatg ctgacccgcg gcggcggggt gaaagtcctc
     1381 gacttcggtg tcgccaagcg gctgaccgcg ccggtgatcg ccgccgagcg gatcaccgag
     1441 gacgtcgcgc ccgacgcggc gagcagcacg ctggccggca cgctgcccta catgtcaccc
     1501 gagcagctga cgctcggcgc cgtcgatcac cgcgccgacg tgtgggcggc cggcatcatg
     1561 ctgttcgagc tggtcgccgg cgcccacccg gtgctcgacc cgagcgcgcc gccggccgcg
     1621 ctgatcgcga tcgccgaccc cgacgtcgcg atgccgtgcg cgaccgagcg cctgcccgag
     1681 atcgggtcgc tcgccgcggt catcgaccgc tgcctgatca agcagcccga gcaccgcacc
     1741 gccagcgcgc gcgtgctgct cgccgagctc gacggcctgg cgcccggccg ccgcagcgtg
     1801 gcggtcggcg acgacggcag c
//
LOCUS       DASVOR010000743         4814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_36364,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000743 DASVOR010000000
VERSION     DASVOR010000743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4814
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36364"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1460
                     /locus_tag="VF516_14665"
     CDS             <1..1460
                     /locus_tag="VF516_14665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108971.1"
                     /translation="GYARRMSRRLVSPSLARALGIGILAALAALGALPDLERTASANG
                     RPPGTSSIVFRRGHESDIIAGLTFGMAISHDGGATWHWMCDDALGIGNSAYDPIYSYT
                     PAGTLFATTLGGLIAMRDGCTFNPAPSGKAFVSAHALGPDGAFYYAAAQTAMNGFSSD
                     FKIYRSIDDGMTFPVSAQPDDPGDVNVLWQSIAVAPSNKLVVYLSGSRYIPVSPGSTE
                     TRRDYLLYRSDDGGASWAPQTPPAPTTQGLTLMPPNSLIHIVGIASDDSKHVYARVEY
                     IDGMTTDALYVSNDFGASWTQIHSQPDRFIAFVARAALHMGHHDLLTTTLKFGTEISH
                     DDGKTWAPLANAPHINTLTENAAGELWAGTQNYGIGQAQSDDAGIMKTTDLSAWTKVL
                     RYQDLVGPVAGCGADTVQQKTCNQSTLWCGVCAQLGCTPSASYVCPIAASEAPVTPPR
                     TRGGCCDAGSGAGGPLALTLSVATLLWRPRRRSSR"
     gene            1457..2485
                     /locus_tag="VF516_14670"
     CDS             1457..2485
                     /locus_tag="VF516_14670"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8108972.1"
                     /translation="MTQILGPLDRLAYRSQHWSLLLQATLLQEATRLVSRLPRPRLSG
                     AEVRAVLRRREQLHARDLANVEAGLYPRELLFDIPVGRYMRALPRLLRDTPRVVRRRK
                     AGAFKDIPEVDKQRYPAYYRRTFHWQTDGYFSDHSAEVYELGVELLFRGTADVMRRQI
                     IPPVTHAIRAAGGASRVRLLDVACGTGRTLHQLAVAHPALRLYGIDLSPAYVRLARRR
                     LADVAEVALAVENAEALPFADGAFDVVTSVYLFHELPRNTRRAVAREMLRVVRPGGLV
                     VLEDSAQLAESAEIATALHSFPEEFHEPFYADYLEDDLAELLREVGFSVESSEPQFVA
                     KVVVARRP"
     gene            complement(2497..3105)
                     /locus_tag="VF516_14675"
     CDS             complement(2497..3105)
                     /locus_tag="VF516_14675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108973.1"
                     /translation="MYNPTAIDGSWAHGPCPPLASNSVQLQVLKQKAAKAARSLVGKY
                     SNRLRALDAKFSLHIDFTKIPESRLDAHYDIRHRIAHDQGLLAPDDPSIEPRVVLGNV
                     VSITEADWKAMISDFLVAVAEVDRASKSSLIADHGIVLYVSQVVTETRTKGIGLRIGT
                     IRQMISDRWKLSVSSKDIESALGALGIPILEPHEKLRRRIVK"
     gene            3674..4096
                     /locus_tag="VF516_14680"
     CDS             3674..4096
                     /locus_tag="VF516_14680"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8108974.1"
                     /translation="MKIERATHVDDELVTALTRLIPQLNTNAPLPTHDDLAMMLASPS
                     TYLLIACDSDIVGTLTLTLFRTLVGLQGRINTVIVDAAARGHGIGEALTREAMRISRD
                     AGVRRITLSSRLDREPAHRMYLRCGFELTGSRQFHMTL"
     gene            4260..>4814
                     /locus_tag="VF516_14685"
     CDS             4260..>4814
                     /locus_tag="VF516_14685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108975.1"
                     /translation="MRQERWFRGGDGSSAFDWQRPAQDLDVPLDVARALYLRATRLVG
                     DPRRAEVLYQRWLRDAAAARPPVTPAPVPGRRTRVEHEARDQRQPRPENAATVGLGKW
                     TRALLETEIDEAVPGAQEVHRVMAALSAGAVRPSSARPSDSLREDDAQTSTSASTPTA
                     FPHNHTGPWDAASQAASVQGQLVAL"
BASE COUNT          838 a         1688 c         1457 g          831 t
ORIGIN      
        1 cgggctacgc tcgccgcatg tcccggcggt tggtatcccc gtcgctcgcc cgcgcgctcg
       61 gcatcgggat cctcgctgcc ctcgccgctc tcggcgcgct gcccgacctg gaacgcacgg
      121 cgtcggccaa cggccgcccg cccgggacct cgtcgatcgt gttccgccgg ggccacgaga
      181 gcgacatcat cgccggcctg acgttcggca tggcgatctc gcacgacggc ggcgcgacct
      241 ggcactggat gtgcgacgac gcgctcggca tcggcaacag cgcgtacgat ccgatctact
      301 cctacacgcc cgcgggcacg ctgttcgcca ccacgctggg cggcctcatc gcgatgcgcg
      361 acggctgcac gttcaacccg gcaccgtcgg gcaaggcgtt cgtgtcggcc cacgcgctcg
      421 gtcccgacgg cgcgttctac tacgcggcgg cgcagaccgc gatgaacggc ttctcatccg
      481 acttcaagat ctacaggagc atcgacgacg gcatgacgtt cccggtgtcc gcgcagcccg
      541 acgatcccgg cgacgtgaac gtcctgtggc agtcgatcgc cgtcgcgccg tccaacaagc
      601 tggtcgtcta cctcagcggc tcccgctaca tcccggtctc gcccgggagc accgagacca
      661 ggcgtgatta cctgctgtat cgcagcgacg acggcggcgc cagctgggcc cctcagactc
      721 cgcctgctcc gaccacccag ggtctcaccc tgatgccccc gaactcgctg atccacatcg
      781 tcggcatcgc cagcgatgat tcgaaacacg tctacgcgcg cgtcgagtac atcgacggca
      841 tgaccaccga cgccctgtac gtcagcaacg acttcggcgc cagctggacg cagatccaca
      901 gtcagccgga tcggttcatc gcgttcgtgg ctcgcgccgc gcttcacatg ggacaccacg
      961 acctcctcac caccaccttg aagttcggca ccgagatctc gcacgacgat gggaagacct
     1021 gggcgccgct cgccaacgcg ccccacatca acaccctgac cgagaacgcg gccggtgagc
     1081 tgtgggcggg cacccagaac tacggcatcg gccaggccca gtccgacgac gctggcatca
     1141 tgaagaccac cgacctgtcg gcctggacca aggtcctgcg ctaccaggac ctggtgggcc
     1201 cggtggccgg ctgcggcgcc gataccgtcc agcagaagac gtgcaaccag agcacgctgt
     1261 ggtgcggcgt ctgcgcgcag ctcggatgta ccccgtcggc ctcctatgtc tgtccgatcg
     1321 cggccagcga ggcgccggtc accccgccga ggaccagggg cggatgctgc gacgccggct
     1381 cgggcgccgg cggtccgctg gcgctcacgc tgtcggttgc tacgctcctg tggcggcccc
     1441 gccgccgatc ctcccgatga cccagatcct cggcccgctc gaccggctcg cgtatcgctc
     1501 gcagcactgg tccctgctgc tccaggcgac cttgctgcag gaggcgacgc gcctcgtgtc
     1561 gcggctgccg cggccccggc tgtcgggcgc cgaggtccgc gccgtgctgc gccgccgcga
     1621 gcagctccac gcccgcgatc tcgccaacgt cgaggccggc ctgtacccgc gcgagctgct
     1681 gttcgacatc ccggtcggcc gctacatgcg cgccctgccc cgcctgctgc gcgacacgcc
     1741 gcgcgtggtg cgccgcagga aggccggtgc gttcaaggac atccccgagg tcgacaagca
     1801 gcgctacccc gcctactacc ggcggacctt ccactggcag accgacggct acttcagcga
     1861 ccactcggcc gaggtctacg agctcggcgt cgagctcctg ttccgcggca ccgccgacgt
     1921 catgcgccgc cagatcatcc cgccggtgac ccacgcgatc cgcgcggccg gcggcgcgtc
     1981 gcgcgtccgc ctgctcgacg tcgcgtgcgg caccgggcgc acgctgcacc agctcgcggt
     2041 cgcccacccc gcgctgcggc tgtacggcat cgacctgtcg cccgcctacg tccggctcgc
     2101 gcgccggcga ctcgccgacg tcgccgaggt cgcgctcgcg gtcgagaacg ccgaggcgct
     2161 gccgttcgcc gacggcgcgt tcgacgtcgt gaccagcgtc tacctgttcc acgagctgcc
     2221 gcgcaacacc cgccgcgcgg tcgcccgcga gatgctgcgc gtcgtccgcc ccggcggcct
     2281 ggtcgtcctc gaggactcgg cccagctcgc cgagagcgcc gagatcgcga ccgcgctgca
     2341 cagcttcccc gaggagttcc acgagccgtt ctacgccgac tacctcgagg acgacctcgc
     2401 cgagctgctc cgcgaggtcg ggttctccgt cgagtcgagc gagccgcagt tcgtcgccaa
     2461 ggtcgtcgtc gcccgccggc cgtaacgtat gctgtcttat ttcacaatcc gccgcctgag
     2521 cttttcatgt ggttccaata ttggaatgcc aagtgcacca agcgcagact caatgtcctt
     2581 actagacact gacaatttcc atcgatcaga tatcatttga cgaatagtac caatcctcaa
     2641 cccaatgcct tttgttcggg tctcggtaac tacttgcgat acatacaaaa caattccatg
     2701 atcagctatt aagctggact tacttgcgcg atccacttca gccaccgcca ctaagaagtc
     2761 agagatcatg gccttccagt ccgcttctgt gatggatacg acattaccaa gaactacacg
     2821 cggctcgata ctggggtcat ctggtgcgag cagaccctga tcatgtgcaa tgcgatggcg
     2881 tatgtcgtag tgtgcatcca atcttgattc tggtatcttc gtgaagtcga tatgcaatga
     2941 aaatttggca tcaagcgccc gaagccgatt actgtacttg cctacaagac tccgcgcggc
     3001 cttggcagct ttctgcttca gtacttgcag ttgaacggaa ttgcttgcaa gtggtgggca
     3061 cggcccgtgg gcccatgagc catcgatcgc ggtgggattg tacaatgaac acaatataca
     3121 gtatgagctc agcgaagtct cgaatgctga gcatgaataa agaacagagg tgcgacgaag
     3181 tgtagctaaa ttcctatcga gagacgcccg aagcgccgag agcggaatag ttaattggct
     3241 ctgttcgcca gagtcaacag atggccagag tgtatccaca cgtggatcgc tgctcgttgt
     3301 acctgattta gcgagcgctt ccaagatgct agcagttcga ccataggcat acttcgtgcg
     3361 ccagtagagt tgattgacgc tcgttatctc agccttgaat atgtgaaacg gttgaagatg
     3421 gctatctggc ttcaggtcct aattgtcgca aaactccgct gggatagctc ttagaccggc
     3481 aaatattttt cgcgagatgc tcccaactat tcgctcgatc gtccgatacc tgcagctcga
     3541 tccgcttgtt accgccaggg agcgggcaac gtgactgctg ccgcgcctgg atctccgaag
     3601 ttttcactgt gcgccgcact gtgcgcggtc gttgtatctc acggtcgccg cgccgcgggc
     3661 gatactcggg acaatgaaga tcgagcgcgc cacacacgtc gacgacgaac tcgtcaccgc
     3721 cctcacgcgg ttgatcccgc agctgaacac gaacgcgccg ctccccaccc acgacgacct
     3781 cgcgatgatg ctcgccagtc catcgacgta cctcctgatc gcctgcgatt cggacatcgt
     3841 tggcactctg acactcacgc tgttccgcac cctggtcggc ctccagggcc gcatcaacac
     3901 cgtcattgtg gacgcggccg cgcgcggcca cggcatcggc gaggcgctga cccgcgaggc
     3961 gatgaggatc tcacgtgacg ccggcgttcg ccggatcacg ctgagctcgc gcctcgaccg
     4021 agagcctgcc caccggatgt atctccgctg cggcttcgag ctgaccggga gccggcagtt
     4081 tcacatgaca ctgtagtagc ctcgtgccct gcggcaccgt gtgggctcag gtgagacgcg
     4141 caaacacgat cagggccgag cgctcccggc aaggtacggc aacgcaggcg ctcattcacc
     4201 tgcacagcgc gtaggatgca cgcacgtcct tcaggcggca ggaacccagc aggagtgtga
     4261 tgcgtcaaga gcggtggttt cgcggcggtg atgggtcgtc agcgttcgac tggcagcgtc
     4321 cggcgcagga tctcgacgtt ccgctggatg ttgcgcgcgc gctgtacctt cgcgcaaccc
     4381 ggctcgtagg cgacccgcgt cgagcggagg tgctctacca gcgctggctg cgcgacgctg
     4441 cggcagcccg accgcctgtc acgccagcac ccgtgccagg ccgccggact cgcgtcgagc
     4501 acgaggcacg agatcagcgg caacctcggc cggagaacgc cgcaaccgtc ggtctcggga
     4561 aatggacacg cgcgctgctg gaaaccgaga tcgatgaggc tgtgcccggc gcccaagagg
     4621 tccatcgcgt gatggccgcg ctgagcgcgg gcgccgtccg acccagcagc gccagaccat
     4681 cggacagcct gcgcgaggac gacgcacaga cgagcacaag cgcaagcacg cctacggcgt
     4741 tcccgcacaa tcacaccggc ccctgggatg cggcatcgca ggccgcttcc gtacagggac
     4801 agctggttgc actc
//
LOCUS       DASVOR010000744         1726 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_503895,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000744 DASVOR010000000
VERSION     DASVOR010000744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_503895"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="VF516_14690"
     CDS             <1..268
                     /locus_tag="VF516_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8108976.1"
                     /translation="FVGLFGTVVGIMNAMSKFKGDEEVKFAMVAGPISEALIVTAVGI
                     GVAVGAVILFNFFSQRANRIATEMKLLTDEFLELLLDTPRNPGT"
     gene            293..799
                     /locus_tag="VF516_14695"
     CDS             293..799
                     /locus_tag="VF516_14695"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8108977.1"
                     /translation="MAIGKLPDEDGGGDEAGEAIFAEINITPLTDVFLVMVIIFMVSA
                     LAVQVEARREKHKVKEEQQQAEVEKKSGLKVNLPSGQAQEIDVKEQSLVLVIPISGDV
                     SVGGKIMRDDQLDAVFHAAFQKDKKTQVVLKADRGVAHGRVVNIMERAKQAGLTHLAI
                     GTSGGGGG"
     gene            complement(855..>1726)
                     /locus_tag="VF516_14700"
     CDS             complement(855..>1726)
                     /locus_tag="VF516_14700"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEX8108978.1"
                     /translation="ARTLVLGPPALEPAERQARLATAAATLGTTPEDVESLLWIDLAM
                     ERPVVLPEGRPRETELAAFANLERIQRCVRRAHDLQLRVWDRATELVRTVARYGLISQ
                     IRCDGDATVLDVMGPLALFHATIVYGRALAQLVPLLAEHPRYELDIRCRISGEEARLH
                     VAPPVWLPPVPASRRAPSTAERLASDLEALGHAVEREPPPIASGAHLLFPDLVLDRGG
                     ARWFIEVLGFATRDYLAAKLDRYRGAGTPRVVLCADLATAPNCELSAQVCGFTRHVEV
                     DELLATIDGGSGG"
BASE COUNT          273 a          623 c          603 g          227 t
ORIGIN      
        1 gttcgtcggc ttgttcggca ccgtggtcgg catcatgaac gcgatgagca agttcaaggg
       61 cgacgaggag gtcaagttcg cgatggtggc cggcccgatc tccgaggcgc tgatcgtcac
      121 cgcggtcggc atcggcgtcg cggtcggcgc ggtcatcctg ttcaacttct tcagccagcg
      181 cgccaaccgg atcgccacgg agatgaagct cctgaccgac gagttcctcg agctcttgct
      241 cgacacgcca aggaatcccg ggacatgacc aagcgcgcga aaggcgatgg cgatggcgat
      301 cggtaagctg cctgacgagg acggcggcgg ggacgaggcc ggcgaggcga tcttcgccga
      361 gatcaacatc accccgctga ccgacgtctt cctcgtgatg gtgatcatct tcatggtgag
      421 cgcgctcgcc gtgcaggtcg aggcccggcg cgagaagcac aaggtcaagg aggagcagca
      481 gcaggccgag gtcgagaaga agagcggcct caaggtcaac ctgccgtccg gtcaggccca
      541 ggagatcgac gtcaaggagc agtcgctggt cctggtcatc ccgatctccg gcgacgtctc
      601 ggtcggcggc aagatcatgc gcgacgacca gctcgacgcc gtgttccacg ccgcgttcca
      661 gaaggacaag aagacccagg tcgtgctcaa ggccgatcgc ggcgtcgccc acggccgggt
      721 cgtcaacatc atggagcgcg ccaagcaggc cggcctgacc cacctcgcga tcggcaccag
      781 cggcggcggc ggcggctgat ccatcccggc atcccggcat cccggggtcc cggcatcccg
      841 gcgagcgctc gccatcatcc gccggatccg ccgtcgatcg tcgcgagcag ctcgtcgacc
      901 tcgacgtggc gggtgaagcc gcagacctgc gccgagagct cgcagttcgg tgcggtggcg
      961 aggtcggcgc acagcacgac gcgcggcgtc ccggcgcccc ggtagcggtc gagcttggcc
     1021 gccaggtagt cgcgggtcgc gaaccccagc acctcgatga accagcgcgc gccgccgcga
     1081 tcgaggacga gatcggggaa caggaggtgc gcgccgctcg cgatcggcgg cggctcgcgc
     1141 tccaccgcgt gacccagcgc ctccagatcg ctcgccaggc gctccgccgt gctcggcgcc
     1201 cggcgcgagg ccgggaccgg cggcagccac accggcggcg ccacgtgcag ccgcgcctcc
     1261 tcgccgctga tccggcagcg gatgtcgagc tcgtaccgcg gatgctcggc gagcagcggc
     1321 accagctggg ccagcgcgcg gccgtagacg atcgtcgcgt ggaacagcgc gagcggcccc
     1381 atcacgtcga gcaccgtcgc gtcgccgtcg caccggatct gcgagatcag cccgtagcgc
     1441 gccaccgtgc gcaccagctc ggtcgcgcgg tcccacaccc gcagctgcag gtcgtgcgcg
     1501 cgccggacac agcgctggat ccgctccagg ttggcgaacg cggccagctc ggtctcccgg
     1561 ggccggccct ccggcagcac caccggccgc tccatcgcca ggtcgatcca cagcagcgat
     1621 tcgacgtcct cgggcgtggt ccccagcgtc gccgccgcgg tcgccagccg ggcctggcgc
     1681 tcggccggtt ccagcgccgg cggtccgagc accagcgtgc gcgccc
//
LOCUS       DASVOR010000745         3195 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_386922,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000745 DASVOR010000000
VERSION     DASVOR010000745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386922"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VF516_14705"
     CDS             <1..278
                     /locus_tag="VF516_14705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108979.1"
                     /translation="LAFLVIAVVVAWRVFRVPLLLNIGTGYAAQQTCACLYVSHRTLD
                     SCLGDLEPLARKLISVRPGADEVTASGFALVTATAHYEKGFGCALRD"
     gene            complement(326..1126)
                     /locus_tag="VF516_14710"
     CDS             complement(326..1126)
                     /locus_tag="VF516_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108980.1"
                     /translation="MARKLLALMVSVAPACLVRELPVDPGNASVTLSGPWIQGQPGSA
                     ALEWPNKELCDEEDWSCTGGSRLTMTVMSVQCSGCDVLDDPTGRTALNGTSFVAMATT
                     DGPILVTADLRFDATGEIRRVVASATGDHEVMLEAPCRLLDPAVPINFDVPDSQLRDC
                     TTTRRPDDRVVVFPLVRTYHGALRLPFGCYDLCSTTWFHKFRSQSSIAITPPPVDWGH
                     SSAVHADGYSDGSFAILPPLGSTSTVSLSAPLASGEISTVSVAIPPVQ"
     gene            1370..2899
                     /locus_tag="VF516_14715"
     CDS             1370..2899
                     /locus_tag="VF516_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108981.1"
                     /translation="MTVRLVAGVMLVSVCGTPPAPPIITTPIAATLVGGSLVLDGARF
                     GERDPSSKLVYVGGTTPSTDPAVTAWADTSITVVLPASARSGPLAVQTADGTSLPVQL
                     EVYAYDFFAVPPTPSTNASPLAVAVDAAHRVWLTSEFQRGELHELDPTAGSPRVSAFP
                     VALPPPPGPFATRIFGDQRTEISEIAESIIVDDHGRVWASQGGGLLYDASHGMFANHS
                     RIVMHDPATATTSIWNMPGDHNEVTGLAWDAAAQRLWFAQSGFDAGAAIGSFDPAQVA
                     PDPCSPACFDFATPVPPTGFTFRALPETQAYPTKLLLDQDGPGGSVGHIWYSAYLANY
                     LGRLDVATGAVDEVPLPPSIGPQGAFWHSSGPWDLVATAGGGILFNEQFDSRIGRLDA
                     ARVRRGDPACTTPGGDSNPCVSLVRVPGADLATELAHSITLDASGRLWYSVHGPNTAA
                     GKASLGIALPDLSAIVRLPPLPGAPTDPLANDGIVIDPTTGDIWIAEYFRHRLGRLRR
                     I"
BASE COUNT          493 a         1138 c         1051 g          513 t
ORIGIN      
        1 tgctcgcgtt cctggtgatc gccgtcgtcg tcgcctggcg cgtgtttcgc gtgccgctgc
       61 tcctgaacat cgggaccggc tatgccgcgc agcagacctg cgcctgcctg tacgtcagcc
      121 accgcaccct cgacagctgc ctcggcgatc tcgagccgct cgcgcgcaag ctgatctccg
      181 tccgcccggg cgccgacgag gtcaccgcca gcggcttcgc cctggtgacc gccaccgcgc
      241 actacgagaa gggcttcggc tgcgcgctgc gcgactgacg ctcgtcggcg cgcgcgccgg
      301 cgatgccgcg gaaccgcgac gccattcact gcaccggcgg gatcgcgacc gataccgtcg
      361 agatctctcc gctcgcgagc ggtgcgctca gcgataccgt cgaggtcgat cccagaggtg
      421 gcaagatcgc aaacgagcca tcggagtagc cgtcggcgtg aactgcggag ctgtggcccc
      481 agtcgaccgg tggcggcgtg atggcgatcg aggattgcga gcggaacttg tggaaccagg
      541 tagtggagca cagatcatag caaccgaacg gaagccggag ggcgccgtga taggtgcgaa
      601 cgaggggaaa caccacgacg cgatcgtcag gccgccgtgt ggtggtgcag tcgcgaagct
      661 gcgaatccgg gacatcgaag ttgatcggca cggcgggatc gagcagccgg cacggtgcct
      721 cgagcatgac ctcgtgatcg cccgtggccg acgccacgac gcgccggatc tcgcccgtgg
      781 cgtcgaaccg gaggtccgcc gtcacgagga tcggaccgtc ggtcgtcgcc atggcaacaa
      841 agctggtgcc attcagcgcc gtgcggccgg tcggatcgtc caggacgtca catccgctgc
      901 actggacgct catcaccgtc atcgtcagcc tcgagcctcc cgtgcacgac cagtcttctt
      961 cgtcgcacag ctccttgttg ggccactcca gggcggcgga acctggctgt ccctggatcc
     1021 acggtccgct cagcgtcacc gacgcattcc cggggtccac cggcagctcc ctcacgaggc
     1081 atgcaggagc gaccgacacc atcaacgcca ggagcttcct cgccatatcc ccgaccatag
     1141 caatcgtcgg accgcagcgc tcgtcacgcg atctccgacg ggtgcagccg tcgagacggt
     1201 tggattcgcc caggctgatg agatcctgac accttcgccg gaggaaatcg cggaatgcgc
     1261 gcgatcggag gtggcagcgc gacgcgctcg ggtgtcgatc cgatatgatg gatccaccct
     1321 gcgcgcgcgt tctctcgggc gagtcatctg ccatcccgga ggtggcgtct tgaccgtccg
     1381 gctggttgcc ggcgtgatgc tggtgtcggt gtgcggcacg ccgcccgcgc ctccgatcat
     1441 caccaccccg atcgccgcaa ccctggtcgg cggctcgctc gtcctggacg gcgcgcgatt
     1501 cggcgaacga gatccgagca gcaagctcgt ctacgtcggc gggaccacgc cgtcgaccga
     1561 cccggccgtg acggcgtggg cggacacgtc gatcaccgtc gtgctgccgg cgagcgcacg
     1621 gtcgggtccg ctcgcggtcc agacagcgga cgggaccagc ttgcccgtcc agctcgaggt
     1681 ctacgcatac gacttcttcg cggtgccgcc gacgccgagc accaacgcct caccgctggc
     1741 ggttgccgtc gatgccgcgc accgtgtgtg gttgacctcg gagttccagc gcggtgagct
     1801 tcacgagctc gatccgaccg ccggctcgcc gcgggtatcg gcgttcccgg tcgcgctccc
     1861 gccgcctccc ggtccgttcg cgacccggat cttcggcgac caacgcaccg agatcagcga
     1921 gatcgccgag tcgatcatcg tcgacgatca cggaagagtc tgggcgtccc agggcggtgg
     1981 gttgctctac gacgcgagcc acggcatgtt cgcgaaccac agccggatcg tcatgcacga
     2041 tccggcgacc gcgacgacct cgatctggaa catgcccggc gatcacaacg aggtgacggg
     2101 cctggcgtgg gatgcggcag cacagcgact ctggttcgca cagagcgggt tcgacgccgg
     2161 cgcggcgatc ggctcgttcg atcccgcgca ggtcgcgccc gatccttgct cgccggcatg
     2221 cttcgatttc gccacgccgg ttccgccgac cggattcacg ttccgcgcac taccggagac
     2281 ccaggcgtat cccacgaagc tgctgctcga tcaggatggt cccggcggct cggtcggtca
     2341 catctggtac agcgcgtacc tcgcgaacta tctgggccgg ctcgacgtcg cgaccggcgc
     2401 ggtcgacgag gtgccgctcc ccccgagcat cggtccgcaa ggcgcgttct ggcactcgtc
     2461 gggaccgtgg gacctcgtcg cgaccgccgg cggcgggatc ctgttcaacg aacaattcga
     2521 ttcgcggatc ggccgattgg atgcagcgcg tgttcgcagg ggggatccgg cgtgcacgac
     2581 acccggcggc gacagcaatc cgtgcgtctc gctggtccgg gttcccggcg cagatctcgc
     2641 gaccgagctc gcacactcga tcaccctcga tgcctccggc cgcctctggt actcggtgca
     2701 cggtccgaac accgcggccg gcaaggcctc gctcggcatc gcgctgccgg atctctcggc
     2761 catcgtgcgg ttgccgccgc tcccaggcgc gccgaccgat ccgctcgcaa atgacgggat
     2821 cgtcatcgac ccgaccacgg gcgacatctg gatcgcggag tacttccgtc atcgcctcgg
     2881 ccggttgcgg cggatctgac tcatttgggc cagtaagaac caacgtagcc cgaacttcga
     2941 gatgatcaag cgtgctccgg gccctgcagg tcttcgaccc ggccgcgcgc tcccagagcc
     3001 atgtacgggc gggcgcggac cacctcgaga tcgcgcacgc tcgctgccgc cgcaggatca
     3061 tcgcctcgcc cgggacgcgc cgccgatgaa ggtcgggctg ttctaccgac cccggccgac
     3121 gcccagcacc atcttcggcg cgggcatcgc cgcggacgac ttcgtccact ccctgctgcg
     3181 gcacgggacc tgcgc
//
LOCUS       DASVOR010000746         5564 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_95356,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000746 DASVOR010000000
VERSION     DASVOR010000746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95356"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VF516_14720"
     CDS             complement(<1..604)
                     /locus_tag="VF516_14720"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108982.1"
                     /translation="MGNVLAFCEFNGAQLRSSALANLSFARQAAQAHGGEVIALLVGA
                     GASAAGPEAAKYAPKVVAADDPRLAHYLAETFAPVVAEVARAQGATLISATATAVGKD
                     LAPRVAGLLDAGMASDIAKLVGKDTFVRPVLAGNAYSTVQVSTPIIVVTPRQSEFEPA
                     AELGSPGAVTTAAPGNVDALGAEFVRLDAQKSDRPELSDAK"
     gene            complement(613..1440)
                     /locus_tag="VF516_14725"
     CDS             complement(613..1440)
                     /locus_tag="VF516_14725"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEX8108983.1"
                     /translation="MKILVPVKRVPDPTQKAKVKDGHIDLTGVPWIVNTFDEYAVETA
                     LRLLENGAENTRLPGSEIVVVSLGPDDAAKELRTCLAMGADRAIHVVTSDAEIDSLTV
                     VKVLSAIVAAEKPDTVIMGKQAVDGDANQVGQMLAAKLGWPQATFAASVELGADKASF
                     TVGREVDGGVEYKKVPLHSVITVDLRIVGPRAVKNGVTPESHNYSGEGARLPALKGIM
                     AAKKKPMAQAKLADYGAAPSPVIKEQSVALPPPRTAGQIVPDIATLVKKLADEAKVL"
     gene            complement(1437..2207)
                     /locus_tag="VF516_14730"
     CDS             complement(1437..2207)
                     /locus_tag="VF516_14730"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8108984.1"
                     /translation="MTVHSSPDRPERGDRGGGDRGGGDRGGGDRSGDKRERILVAAER
                     IFARHGFFAARVSEIAREAGVADGTIYLYFKSKDDLLISLFENRMKQVNEQLRRAIAN
                     KPPGEQLRAFIRTYLQLVSDEPTAAEVLTIELRQSSKFMKEYDNPQFADFLRMLGGII
                     ADGQARGELDTAIPSHIAARMIFGIVDELALAWVLAKQPLRGAAAPAASAAIPGPAPG
                     TRPKKFDIVRAADWVVAMVTGGLEQGQANKTPKKETPS"
     gene            2340..3233
                     /locus_tag="VF516_14735"
     CDS             2340..3233
                     /locus_tag="VF516_14735"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8108985.1"
                     /translation="MIACAPLLITGACEKKTKPNDTGAVAALDNAVAPPAAEGPADTS
                     PLQGIDVSKLEGDKSQLFYKLINSLKSPCGKAHSLRTSFSSDTSCKRAPFAVRYVLAM
                     VEDEGTEDRVREEYARKYERPAQPVKLDTSRAPHIGANDPPIKLVEFYDYECPHCQAF
                     KAQLEQVEADKSAEVGVSFMMFPIESKHPEARSAAQAAIAASQQGKFKEMHDKLFVPQ
                     AAHGRDAVLGYAKEIGLDIAKFQKAYDDASPQVTTDLQQGQDAGVDATPTLFFNDRKY
                     EGPMVAKYIEMWIDEDLAVNR"
     gene            3242..3796
                     /locus_tag="VF516_14740"
     CDS             3242..3796
                     /locus_tag="VF516_14740"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8108986.1"
                     /translation="MRPTAMRAVFLRPGFASGLAAAAAALLLATPARADVLKVGDRLA
                     ELDVATDGNGKPVKLKSFKGKWVVLTVGADWCKPCAKELPTWDKLAGELKGKVVFLAV
                     DIDDEIDVGKKFHDKLKLHNMVRAYLPSEKSGVAASYGSDHMPSTFVGDPQGVVRLVR
                     EGFEAGDADGEYKKFKDQLAKLVK"
     gene            3951..4535
                     /locus_tag="VF516_14745"
     CDS             3951..4535
                     /locus_tag="VF516_14745"
                     /inference="COORDINATES: protein motif:HMM:NF018446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HEX8108987.1"
                     /translation="MSKGRLEAFSDGVIAILITIMVLELKVPHSTDPHALSAMLPVFL
                     AYVLSFVYLGIYWNNHHHMLQVAQRVSGRVLWANLHLLFWLSLVPFVTGWMGENGNAP
                     FPTACYGAVLLLAGVAYLILQRSIIVAEGEGSKLAAAVGRDIKGKLSAVMYAASIPLS
                     FVHVVIADAIYVMVALMWLVPDPRLARRMQQEGA"
     gene            complement(4834..5274)
                     /locus_tag="VF516_14750"
     CDS             complement(4834..5274)
                     /locus_tag="VF516_14750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108988.1"
                     /translation="MTRAAVLAGMLVAACSRRAPLASCDDNLHGVYTAGPDERWMLVA
                     RRDSLEAYPLFPDVDGPRELEIGPRRIDFTRSPASPAALAGTVHRRYMRRDAICEAQL
                     PVHITRCAGDTLELVLADPAPPLGFSPCRWPGPAPSRLVRWQRE"
     gene            complement(5300..>5564)
                     /locus_tag="VF516_14755"
     CDS             complement(5300..>5564)
                     /locus_tag="VF516_14755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HEX8108989.1"
                     /translation="MKILRSRILEAERERQMASEAASRKSQVGSGDRSEKIRTYNFPQ
                     DRVTDHRIGLTKHNLPAIMDGNLLDIIEALRTHDQAERLKGLD"
BASE COUNT          851 a         1916 c         1905 g          892 t
ORIGIN      
        1 ccttggcgtc cgacagctcc ggccggtcgc tcttctgcgc gtcgagccgg acgaactcgg
       61 cgccgagcgc gtcgacgttg ccgggggcag cggtcgtcac cgcgccgggc gagcccagct
      121 cggccgcggg ctcgaactcg gactggcgcg gcgtcacgac gatgatcggc gtcgacacct
      181 gcacggtgga gtacgcattg ccggccagga ccggccggac gaacgtgtcc ttgccgacga
      241 gcttggcgat gtccgacgcc atcccggcgt cgagcagccc ggcgacccgc ggggcgagat
      301 ccttgccgac cgcggtcgcg gtcgccgaga tcagcgtggc gccctgggcg cgcgcgacct
      361 cggcgacgac cggcgcgaac gtctcggcga ggtagtgagc gagacgggga tcgtcggctg
      421 ccaccacctt gggggcgtac ttcgccgcct cgggcccggc cgcggacgcc ccggcgccga
      481 cgagcagggc gatcacctcg ccgccgtggg cttgcgcggc ttgccgggcg aacgacaggt
      541 tggccagcgc gctcgagcgc agctgcgcgc cgttgaattc acagaacgcg aggacgttac
      601 ccatggcggc tcctacagga ccttcgcctc atcggcgagc ttcttcacga gggtggcgat
      661 gtcggggacg atctggccgg cggtgcgcgg cggcggcagc gcgaccgact gctccttgat
      721 caccggggac ggcgcggcgc cgtagtcggc gagcttggcc tgggccatcg gcttcttctt
      781 cgcggccatg atgcccttga gcgccggcag ccgggcgccc tcgccggagt agttgtgcga
      841 ctccggggtg acgccgttct tgaccgcgcg cggtccgacg atgcggaggt cgaccgtgat
      901 cacgctgtgc agcgggacct tcttgtactc gacgccgccg tcgacctcgc ggccgaccgt
      961 gaacgacgcc ttgtccgccc cgagctcgac cgacgcggcg aacgtcgcct gcggccagcc
     1021 cagcttggcg gcgagcatct ggccgacctg gttggcgtcg ccgtcgaccg cctgcttgcc
     1081 catgatcacg gtgtcgggct tctcggccgc gacgatcgcg ctcagcacct tgaccaccgt
     1141 gagcgagtcg atctccgcgt cgctggtcac gacatggatc gcgcggtccg cgcccatcgc
     1201 cagacaggtc cggagctcct tggcggcgtc gtcgggcccg agcgacacca cgacgatctc
     1261 gctgcccggg agccgggtgt tctcggcgcc gttctcgagc aggcggagcg cggtctcgac
     1321 cgcgtactcg tcgaacgtgt tgacgatcca gggcacgccc gtcagatcga tgtggccgtc
     1381 cttgaccttg gccttctgtg tcgggtcggg gacccgcttg accggaacga gaatcttcac
     1441 gagggcgtct ccttcttggg ggtcttgttc gcctggccct gctccaggcc accggtgacc
     1501 attgccacga cccagtcggc cgcgcgcacg atatcgaact tcttcggtcg ggtaccaggc
     1561 gccggccccg ggatcgcagc ggacgccgcc ggcgccgctg ccccccgcag cggctgcttg
     1621 gccagcaccc aggccagcgc cagctcgtcg acgatgccga agatcatgcg cgccgcgatg
     1681 tggctcggga tcgccgtgtc gagctcgccg cgcgcctggc catcggcgat gatcccgccg
     1741 agcatgcgca ggaagtcggc gaactgcggg ttgtcgtact ccttcatgaa cttgctcgac
     1801 tggcggagct cgatcgtcag cacctcggcc gcggtcggtt cgtccgacac cagctgcagg
     1861 tacgtgcgga tgaaggcgcg cagctgctcg ccgggcggct tgttcgcgat cgcccggcgc
     1921 agctgctcgt tgacctgctt catccggttc tcgaacagcg agatcagcag gtcgtccttg
     1981 ctcttgaagt acaggtagat cgtgccgtcg gcgacgccgg cctctcgcgc gatctccgac
     2041 acccgcgcgg cgaagaaccc gtggcgcgcg aagatccgct ccgcggcaac caggatccgc
     2101 tcgcgcttgt cgccgctccg gtcaccgcct ccacggtcac cgcccccacg gtcaccgccc
     2161 ccacggtcac cgcgctcggg tcgatcgggc gatgaatgaa ccgtcattca gtgctgtaca
     2221 tactccccgt gacgcaggac gtcaagccgt gcgacgacgg gagccggctt gaaacgcatc
     2281 agagcgtgca ctacaacctg cgggtgtcaa acgcacgaat ttcgggacat ttcgtcctca
     2341 tgatcgcctg tgcgccgctg ctcatcaccg gcgcgtgcga gaagaagacg aagccgaacg
     2401 ataccggcgc ggtggccgcc ctcgacaacg ccgtcgcgcc gccggccgcc gagggcccgg
     2461 ccgacacctc gccgctgcag ggtatcgacg tgagcaagct cgagggcgac aagagccagc
     2521 tgttctacaa gctgatcaac tcgctcaagt cgccgtgcgg caaggcccac agcctgcgca
     2581 cctcgttcag ctccgacacg tcgtgcaagc gggcgccgtt cgccgtgcgc tacgtcctgg
     2641 cgatggtcga ggacgagggc acggaggacc gggtgcgcga ggagtacgcc cgcaagtacg
     2701 agcggcccgc ccagccggtc aagctcgaca ccagccgcgc gccgcacatc ggcgccaacg
     2761 accctccgat caagctcgtc gagttctacg actacgagtg cccgcactgc caggcgttca
     2821 aggcgcagct cgagcaggtc gaggccgaca agtccgccga ggtcggcgtc tcgttcatga
     2881 tgttcccgat cgagtccaag caccccgagg cgcgcagcgc cgcccaggcc gcgatcgccg
     2941 ccagccagca gggcaagttc aaggagatgc acgacaagct gttcgtgccc caggccgcgc
     3001 acggccgcga cgccgtgctg ggctacgcca aggagatcgg cctcgacatc gccaagttcc
     3061 agaaggccta cgacgacgcg tcgccccagg tcaccaccga cctccagcaa ggccaggacg
     3121 ccggtgtgga cgccaccccc accttgttct tcaacgaccg caagtacgag ggcccgatgg
     3181 tcgccaagta catcgagatg tggatcgacg aagatctcgc ggtgaatcga taatcggctc
     3241 gatgcgtccc accgccatgc gcgccgtctt ccttcgcccc ggcttcgctt cgggcctcgc
     3301 ggcggccgcc gccgccctcc tgctcgccac ccccgcccgc gccgacgtcc tcaaggtcgg
     3361 cgatcgcctc gccgagctcg acgtcgccac cgacggcaac ggcaagccgg tcaagctcaa
     3421 gtcgttcaag ggcaagtggg tcgtcctcac cgtcggcgcc gactggtgca agccgtgcgc
     3481 gaaggagctg ccgacctggg acaagctcgc cggcgagctc aagggcaagg tcgtgttcct
     3541 cgcggtcgac atcgacgacg agatcgacgt cggcaagaag ttccacgaca agctcaagct
     3601 gcacaacatg gtgcgcgcct acctgccgtc cgagaagtcc ggcgtcgccg ccagctacgg
     3661 ctccgaccac atgccgtcga ccttcgtcgg cgacccgcaa ggcgtcgtcc gcctggtccg
     3721 cgagggcttc gaggccggcg acgccgacgg cgagtacaag aagttcaagg accagctggc
     3781 caagctggtc aagtagcctc ctggccggac aataccgcgg ggctcggcgg aattcgcgtt
     3841 cgaaactggc tcactcgatc cgcggcgcaa accacaacta cgacgaccgc tggcatccca
     3901 cgctgacctt caatctgctc acgggcctgt gaagcggtac attcggcgcg atgagcaaag
     3961 gtcgcctcga agccttcagt gatggcgtca tcgctatcct gatcacgatc atggtgctcg
     4021 agctcaaggt gccccacagc acggatccac acgcgttaag cgcgatgttg cccgtgttcc
     4081 tggcctacgt gctcagcttc gtgtacctgg gtatctattg gaacaatcac catcacatgc
     4141 tccaggtggc ccagcgcgtg agcggccgcg tgctgtgggc gaatctgcat cttctgttct
     4201 ggttgtcgct ggtgccgttc gtcaccgggt ggatgggcga aaacggtaac gcgccgtttc
     4261 cgacggcatg ctacggcgcg gtgctgctgc tggcgggggt cgcgtacctg atcctgcagc
     4321 ggagcatcat cgtcgccgag ggcgagggtt cgaagctggc tgctgcggtg ggccgtgata
     4381 tcaagggcaa gctctcggcg gtgatgtacg cagcctcgat tccgctctcg ttcgtgcacg
     4441 ttgtcattgc cgacgcgatc tacgtcatgg tggccctcat gtggttggtg ccggatccgc
     4501 gcctcgcgcg gcggatgcag caggaaggcg cgtaaccgat cgcgagcctg ccctgttcgt
     4561 gagcgcgcac gagctcggcg gccgccaccc agcaacggac gtgaagctcg agtcacccga
     4621 agggcaagcg cgcgtcgctt caccagcgga ggcagctgtg agccggggct cgatgaccag
     4681 cagcttgccg agctcgtaga cgtcgtcgaa gaagtcgtcc cagcgctgct cggcgaggtc
     4741 tcgccgcgcg cgcgtcacaa ctcccgggag ctcatcggcc gtcacttctt ccccacgttc
     4801 atgtgggggg cctctctcgt gccgtggcgc atcttactcg cgctgccagc ggaccaggcg
     4861 tgatggggcg gggccgggcc accggcacgg tgagaagccg agcggcgggg ccggatcggc
     4921 gagcaccagc tcgagggtgt cgccggcgca gcgggtgatg tggacgggga gctgcgcctc
     4981 gcagatcgca tcgcggcgca tgtagcggcg atgcacggtg ccggcgagcg cggcggggct
     5041 cgccggcgag cgggtgaagt cgatccggcg cgggccgatc tcgagctcgc gcgggccgtc
     5101 gacgtcgggg aacagcggat aggcctcgag cgagtcgcgg cgggcaacga gcatccagcg
     5161 ctcgtccggg ccggcggtgt agacaccgtg caggttgtcg tcgcacgagg cgagcggcgc
     5221 gcgtcggctg caggctgcga cgagcatgcc ggcgagcacg gccgcgcggg tcatgcgcgc
     5281 gtgctccgcc ggcgggacgt caatcgaggc ccttgagccg ctcggcctgg tcgtgggtgc
     5341 ggagggcctc gatgatgtcg aggaggttgc cgtccatgat cgccggcagg ttgtgcttgg
     5401 tcaggccgat ccggtggtcg gtgacgcggt cctgggggaa gttgtaggtc cggatcttct
     5461 cggagcggtc gcccgagccg acctgggact tgcggctcgc cgcctcgctg gccatctggc
     5521 gctcgcgctc ggcctcgagg atgcgcgagc ggaggatctt catc
//
LOCUS       DASVOR010000747         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_328600,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000747 DASVOR010000000
VERSION     DASVOR010000747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328600"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..968
                     /locus_tag="VF516_14760"
     CDS             396..968
                     /locus_tag="VF516_14760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108990.1"
                     /translation="MDGLLRVLTWICAAIPLVVGLAATRAPAPARAILFGVAVAMLAM
                     YAFIWLWMRPTGFVVAPEGLVIDWPVRRRRIQAAAVTAARILNRDQLQRELGRIVRIS
                     CTHTQSPSGRQTGEPTVSHEGSARAASRWVRAGQDRAGLVELWVSRTDWMVLVECTGR
                     RSLLITPVDRAVRERTGSSRLVSLDRRGCA"
     gene            complement(1084..1236)
                     /locus_tag="VF516_14765"
     CDS             complement(1084..1236)
                     /locus_tag="VF516_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108991.1"
                     /translation="MKRPRMLTGAYAARPHGCEQRHVGLSDGRTWSSSAIQQRLAQLL
                     SELVTP"
     gene            complement(1233..>1834)
                     /locus_tag="VF516_14770"
     CDS             complement(1233..>1834)
                     /locus_tag="VF516_14770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108992.1"
                     /translation="QLAWRSEPPAIATAHARTAELQRRLRRHRRVAWAYTLFDVCASV
                     ALFGIGAYMLIRSPTLPVLVWSISVFVFTAIAMGGAIWSRRDALAASADSTSDFLAAL
                     RLRLDRRERVPRFLLRLAAAEIAFGLAFYAIWSPEWLGRAAKIYGVIALALAAWSRWY
                     RRRLGRERAQLDALCGEPEPPDAPDPPGDGGAAPGTHGA"
BASE COUNT          283 a          624 c          660 g          267 t
ORIGIN      
        1 cggtcgctca tgtcgattca gctcgcgcaa gagctcccag tcgtcgagat cggcgaacac
       61 cggatcgatg tcgcgaatcg ggacgagctc gacctgcagc aacgcatggc cagcgccgcg
      121 agcacgggct gaggaaagtt gtggtcgagc gcgaacaggc gaggtggcac gccgtcgacc
      181 tcaagccgcg cgcttgctca ggttgtcggc gagctggtcc tcgagctcca gcgcttgttc
      241 gatctgcggc gcgctcacgt acggatacag tcgatggatc gccgtcacgt cctaccgcat
      301 ccatatggcc gagcctagca cggcggcgat ccacggactc ggcgcctggc gccatggcac
      361 cctggagaga tggtgacacg atacccgctc gcgcgatgga cggcctgctt cgcgtgctga
      421 cctggatctg cgccgcgatc ccgctggtcg tcgggcttgc agcgacccgt gccccggcgc
      481 cggcacgcgc gatcctgttc ggcgtcgccg tcgcaatgct cgcgatgtac gcgttcatct
      541 ggctgtggat gcgcccgacc ggcttcgtgg tcgcgcccga gggcctcgtc atcgactggc
      601 cggttcgccg gcgccggatc caggccgccg ccgtcaccgc cgcccggatc ctcaatcggg
      661 atcagctgca gcgcgagttg ggccggatcg tcaggatcag ctgcacccat acgcagtccc
      721 cttcagggcg gcagaccggt gagcctaccg tgtcccatga gggatcggcg cgggcggcct
      781 cgcggtgggt tcgggctggc caggaccgcg cgggtctcgt cgagttgtgg gtctcgcgca
      841 cggactggat ggtgcttgtc gaatgcaccg gacgacgatc gctgctgatc acgccggtgg
      901 atcgagcggt tcgtgagcga actggctcgt cgcgcctcgt gagtctcgat cgcaggggtt
      961 gtgcatgatc caaaccctcg ggatccgagc tctaccggcc gaagcgatgg aaggccggcg
     1021 ctcagaccag ccgctgcgta accccgcgag gctcatccat cgcagttgga gcaacgatat
     1081 cgtctatggg gtgaccagct ccgagagcag ctgggcgagc ctctgctgga tcgccgagct
     1141 actccacgtc cgtccatcgg acagcccgac gtggcgctgc tcgcagccgt gcggccgagc
     1201 tgcataggcc ccggtcaaca tcctgggacg cttcaagctc cgtgggtacc gggagccgcg
     1261 ccgccgtcgc ccggcgggtc cggggcatcc ggcggctcgg gctcgccgca cagcgcatcg
     1321 agctgggcgc gctcgcggcc cagccggcgg cgataccacc gcgaccacgc cgccagcgcc
     1381 agggcgatga cgccgtagat cttggccgcg cggccgagcc attcgggcga ccagatcgca
     1441 tagaacgcga ggccgaaggc gatctccgcg gcggcgaggc gcaggaggaa gcgcggcacg
     1501 cgctcgcggc gatcgaggcg cagccgcagc gcggcgagga agtccgaggt ggagtccgcc
     1561 gaggccgcga gcgcatcgcg gcggctccag atcgcgccgc ccatggcgat cgcggtgaac
     1621 acgaacacgc tgatcgacca cacgaggacc ggcagggtcg gcgaccggat cagcatgtag
     1681 gcgccgatgc cgaacagcgc gaccgaggcg cagacgtcga acagggtgta ggcccaggcg
     1741 acgcggcggt ggcgccgcag ccggcgctgg agctccgcgg tccgcgcatg agcggtcgcg
     1801 atggcgggcg gctcgctgcg ccaggcgagc tgcc
//
LOCUS       DASVOR010000748         7262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_240866,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000748 DASVOR010000000
VERSION     DASVOR010000748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7262
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240866"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VF516_14775"
     CDS             <1..192
                     /locus_tag="VF516_14775"
                     /inference="COORDINATES: protein motif:HMM:NF024930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HEX8108993.1"
                     /translation="RGTPVAVPMPGNASVLDVVAGEQHTCAVLADGSVACWGDDRFGQ
                     LGDGILADQTPVAPLLPCP"
     gene            complement(239..7228)
                     /locus_tag="VF516_14780"
     CDS             complement(239..7228)
                     /locus_tag="VF516_14780"
                     /inference="COORDINATES: protein motif:HMM:NF036757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HEX8108994.1"
                     /translation="MIPQWANVRNQSQSQNRVDAGFVLGQGAGAVTLVFCDDQYVRYS
                     GGYEGFVDEGYPKVIAHLAAADGAFPGLPAPLPSGLRGAFAGTDGNLHAFGPPPSDTS
                     QPQLYVSSASPGTLRPLNERWGIVDNKLWDNEFVNAALVAGGKLFLFSGDQYVRYSGT
                     DRTSVDEGYPRKIASSYAKEIGATALAPIVNQGVDAALTLGGTTFYFVQDQVVTSDHP
                     DVAQPLVPRWGLVDNRLQASKKLDAALVAPSGKLYLFAGPQVSVYSGADRTYVDEEYP
                     RAIATDIGGLWPDAFRADLDAAAGFDGRAYLFKGAQHVRISDFRLTRPDAGYPLDNAG
                     KLVDRFDFELGALPDGWRIKQLFDDFSSQTTTVLDTLDIPPADPVAGLARATQWPASE
                     ITALLAIFALAPSALVDARAVVRLARCFELADRIGTTPSRLKSQVWDLVFGAGAPQLQ
                     AAADVLYGLIKVATSPKDWPGVARSLLDPVESARRDALVAYLVENLQRPGPTGPEHLR
                     DANDLYEYLLTDVQMDASSTTSKIVEAINSVQLFYHRALIHLEDLPDPVRVNLKAWWP
                     WMKNYRIWEANRKVFLHPENYIRPELRPVKSPAFEELEQKLLQDEITAITVTDGYQRY
                     LESFHEIAQLRIVGGYRYTFHDDAGAERTGVFTIGVSRKEPPVHYYRLGTLTSEDHID
                     WQPWQKTGITIDSDRVQPVYAFNRLFLFWIEPQPFNATAFSVGSSQTFSSDSDDTKRV
                     RLTLKFSFYNFTKEWVAPPTVVLDPGNASDPAVLPTVFSVAAAADVQLTARNPAPAGN
                     SDQDFIYLTFRFLFWEYQVGQLTAGLDVQRPGPADQGWKLMGFGSLSSPSPQLPFADP
                     MAQFPAQIGIPLREVSAIVPWSAHLESDMGSWFSFDAKGGTFLCRPVNAPPRAPGTIL
                     GRAFPGVSAAFDTLSGDVFVFSTDAGKLGFHHYVHAEHAWRDPVFVGDPAWPWGRSIG
                     VFEKFPDKRIANVIVALSDVTYVLLDPASAPTHFTYPRGTYDATAEHFATDLKVGSPT
                     LDQLVGTGVPSLSWHDAVADGRSLVRAFKLPGANQAVLVTQGGGGGALNVTTASLDDL
                     RKASADQDGHSPFDGWDRLDTVFQVDAPEPRVAFSRGTQLVILTWSSKAWTTAALGVL
                     PDGAPGLSAAFRGVDGKLYFFCGSQYAEVDPANLGGNQVFVPVAARWGRPSLFSWLVS
                     SVDGAVFGPDGKLYLFAAPPDDGSPLRGYCLRYSSFDPAALDKLVLDDPLTTPPRVAD
                     VWLSLFRGQPSVERVTAAFARNGRAYLFGQRGDRTPFAARYTQATQSPFAADSGYPMQ
                     LTEVASNLSADFYAQVLAADQQFSITRLTSHTSEQFGRRLFASGIPGLLSLDTQRLSE
                     LPRFTRLSDAVGRSAAPDELFVNPEFVTEYPGKDASQGLDFASASGFYYREIFFHIPY
                     LIAQALKQEQRFDDAERWYEYVFDPTSQDGNTQFWRYIEFLQDTALTPLQEQIDAYRS
                     DPFDPHKIAELRPTAYRKAFVMSYIDNLLAWGDLLFRQFTRESLGEATMLYVRAADLL
                     GKKPEDLGKRAMALADSLTYEQVRDRTIAAASDDILELENGVPPLSGAAAPQVIPNDS
                     LFNPYFYLPENEQFVELWSRVGDRLFKIRNGLNIDGVKQSLALFAPPVDVMALVQAFA
                     SGAGLAQVLADYSAAVPHYRFAFMLGRARELTGRLSGLGSALLSALEKKDAEELSLLR
                     NTQEHAILDMQLQIKQQQLEAAKQSLTALQEGLKNARARETHYQELLTAGLTAHEQEQ
                     IAQMNVGQQHSQTGNSYSIASSLGSIIPQIGSPFAMTFGGQQIGPALAAMATSFKARG
                     EAASFQSSLAATFGGWDRRSQDWQLQKTLATGDMQQIGRQIKAAEIQVDIANQEIQVQ
                     RKQIKNNEAIDTFMNSKFTSRQLYQFMIGKLSAVYFQTYQLALGYAKAAQRAMQFELA
                     LPESDIQFIGAAYWDSLKKGLTASEQLQLDLDRLEKAHCDANERRMEITRYVSLVQVD
                     PLALVRLKEQGSCEFELTEALFDEDFPGHYCRQIKTVSLSFPAVVGPYHNFNATLTQL
                     GHRTLLTPDKAALKYLLQGPSSSTGQPAAVPAATVLRVDWRPNQQVALSTGTNDAGLF
                     QVDYHDERFLPFEGTGAVSTWRLEVNGVDGPLHRQTLSDVILTVRYTARTGGSAFAET
                     VKSALGGTSRDSAWLLNLASDFPDRWQAFMNKPSDGMVFSVERRRLPGASDRKVTGVY
                     LHYERVDEPVDDLSRQGVTLNGTSIKPGAFKTGLTLPLLEQGQDPARATWKLVPSGGA
                     DKFSTRNLRTILLVVTYATKPSF"
BASE COUNT         1102 a         2416 c         2600 g         1144 t
ORIGIN      
        1 cgcggcacgc cggtggcggt gccgatgccc ggcaacgcca gcgtgctcga cgtcgtcgcc
       61 ggtgagcagc acacctgcgc cgtgctcgcc gacggcagcg tcgcgtgctg gggtgacgac
      121 cgcttcggcc agctcggtga cggcatcctg gccgatcaga cgccggtcgc gccgctgctg
      181 ccgtgcccgt gacgccgccc gccgtcgacg tcgatcggcc aggccggcct cgcccggctc
      241 agaacgacgg cttggtggcg taggtgacga ccagcaggat ggtcctgagg ttgcgggtgc
      301 tgaacttgtc cgcgccgccg gacggcacca gcttccacgt cgcgcgcgcc ggatcctggc
      361 cctgttcgag cagcggcagg gtgagcccgg tcttgaacgc gcccggcttg atcgaggtgc
      421 cgttgagcgt gaccccctgg cggctgagat cgtccaccgg ctcgtcgacc cgctcgtagt
      481 gcaggtagac gccggtgacc ttgcgatcgc tggcgccggg caggcggcgg cgctccaccg
      541 agaacaccat cccgtcggac ggcttgttca tgaacgcctg ccagcggtcc gggaagtccg
      601 acgcgaggtt gagcagccag gcgctgtccc gcgacgtgcc gccgagcgcg ctcttcacgg
      661 tctcggcgaa cgcgctcccg ccggtgcgcg ccgtgtagcg cacggtcagg atcacgtcgc
      721 tgagggtctg gcgatggagc ggaccatcca cgccgttgac ctcgaggcgc caggtcgaca
      781 ccgcgccggt gccctcgaac ggcaggaacc gctcgtcgtg gtagtcgacc tggaacagcc
      841 cggcgtcgtt ggtgccggtg gacagcgcga cctgctggtt gggacgccag tcgacgcgca
      901 gcaccgtggc ggccggcacc gcggccggct ggccggtcga cgagctcggc ccctggagca
      961 ggtacttcag cgcggccttg tccggcgtca gcagcgtgcg gtggccgagc tgggtcaggg
     1021 tcgcgttgaa gttgtggtac gggccgacca cggcggggaa cgacagcgag acggtcttga
     1081 tctgccggca gtagtgcccg gggaagtcct cgtcgaacag cgcctcggtg agctcgaact
     1141 cgcagctgcc ctgctccttg agccggacca gggccagcgg gtcgacctgc accagcgaca
     1201 cgtacctggt gatctccatc cggcgctcgt tggcgtcgca gtgcgccttc tccagccggt
     1261 cgagatcgag ctggagctgc tcgctcgcgg tcagcccctt cttgaggctg tcccagtagg
     1321 cggcgccgat gaactggatg tcggactccg gcagcgcgag ctcgaactgc atggcgcgct
     1381 gggccgcctt cgcgtacccg agcgccagct gataggtctg gaagtacacc gccgacagct
     1441 tgccgatcat gaactggtag agctgccggc tggtgaactt cgagttcatg aacgtgtcga
     1501 tcgcctcgtt gttcttgatc tgcttgcgct ggacctggat ctcctggttg gcgatgtcga
     1561 cctggatctc cgcggccttg atctgccggc cgatctgctg catgtcgccg gtggcgagtg
     1621 tcttctggag ctgccagtcc tggctgcggc gatcccagcc gccgaacgtt gccgccagcg
     1681 agctctggaa cgaggctgcc tcgcctcgcg ccttgaacga cgtcgccatg gctgcgagcg
     1741 ccgggccgat ctgctggccg ccgaacgtca tcgcgaacgg cgagccgatc tgtggaatga
     1801 tgctgccgag cgacgaggcg atactgtagc tgttccctgt ctgcgagtgc tgctgcccga
     1861 cattcatctg cgcgatctgc tcctgctcgt gggcggtgag cccagcggtc agaagctcct
     1921 gatagtgcgt ctctcgcgcc cgggcgttct tgaggccctc ctggagcgcg gtgagcgact
     1981 gcttggcggc ctcgagctgc tgctgcttga tctggagctg catgtccagg atcgcgtgct
     2041 cctgggtgtt gcggagcagg ctcagctcct cggcgtcctt cttctccagc gcggacaaga
     2101 gcgcgctgcc caggccgctc agccggccgg tcagctcgcg ggcgcggccg agcatgaacg
     2161 cgaaccggta gtgcggcacc gcagcgctgt agtccgccag cacctgggcg agcccggcgc
     2221 cggaggcgaa cgcctggacc agcgccatca catcgaccgg cggcgcgaac agcgccagcg
     2281 actgcttcac gccatcgatg ttgaggccat tccggatctt gaacagccga tcgccgaccc
     2341 ggctccacag ctcgacgaac tgctcgttct ccggaagata gaagtacggg ttgaacaggc
     2401 tgtcgttcgg gatcacctgc ggcgcggcgg cgccggacag cggcgggacg ccgttctcga
     2461 gctccaggat gtcgtcgctg gcggccgcga tcgtgcgatc gcggacctgc tcgtaggtca
     2521 gcgagtccgc cagcgccatc gcgcgcttgc ccaggtcctc gggcttcttg cccagcaggt
     2581 ccgcggcgcg cacgtagagc atggtggcct cgccgaggct ctcgcgcgtg aattgccgga
     2641 acagcagatc gccccatgcc agcaggttgt cgatgtagct catcacgaac gccttgcggt
     2701 acgcggtcgg ccgcagctcg gcgatcttgt gggggtcgaa cgggtcgctc cggtaggcgt
     2761 cgatctgctc ctgcagcggc gtcagcgcgg tgtcctggag gaactcgatg taccgccaga
     2821 actgcgtgtt gccgtcctgg ctggtcggat cgaacacgta ctcgtaccag cgctcggcgt
     2881 cgtcgaagcg ctgctcctgc ttgagcgcct gggcgatcag gtacgggatg tggaagaaga
     2941 tctcgcggta gtagaagccg ctggcgctgg cgaaatcgag cccctgtgac gcgtccttgc
     3001 ccgggtactc ggtgacgaac tcggggttga cgaagagctc gtccggcgcc gccgatcgcc
     3061 ccacggcgtc ggatagccgc gtgaaccggg ggagctccga cagccgctgg gtgtccagtg
     3121 acaggagccc cgggatcccg ctcgcgaaca gccgccgccc gaactgctcc gaggtatggc
     3181 tggtcagccg ggtgatcgag aactgctggt ccgccgccag gacctgggcg tagaagtcgg
     3241 cggacaggtt ggacgccacc tcggtcagct gcatcgggta cccggaatcc gcggcgaacg
     3301 ggctctgcgt cgcctgcgtg taccgcgccg cgaacggcgt acgatcgccg cgctgcccga
     3361 acaggtaggc acggccgttg cgggcgaacg ccgcggtcac gcgctcgacg ctcggctgtc
     3421 cgcggaacag gctgagccag acgtcggcca ccctgggcgg agtcgtgagc ggatcgtcca
     3481 gcaccagctt gtccagcgcg gccgggtcga acgagctgta acgcaggcaa tacccgcgga
     3541 gcggcgaccc gtcgtccggg ggcgccgcga acaggtagag cttgccgtcc ggtccgaaca
     3601 ccgcgccgtc caccgacgac accagccagg agaacagcga gggccggccc cagcgcgcgg
     3661 cgaccgggac gaacacctgg tttccgccga ggttggccgg atcgacctcg gcatactggc
     3721 tgccgcagaa gaagtagagc ttgccgtcca ccccgcggaa cgcggcggac agcccgggcg
     3781 ctccgtcggg gagcacgccc agcgcggccg tggtccaggc cttcgaggac caggtgagga
     3841 tcacgagctg cgttccgcgg ctgaacgcga cgcgcggctc gggagcatcg acctgaaaca
     3901 cggtgtccag gcggtcccag ccgtcgaacg ggctgtggcc atcctggtcg gcggaggcct
     3961 tgcgcaggtc gtccaggctg gcggtggtga cgttcagcgc gccgccgccg ccgccctggg
     4021 tcaccagcac cgcctggttc gcgcccggga gcttgaaggc ccgcaccagc gatcgcccgt
     4081 cggcgaccgc gtcgtgccac gacagggacg gcacgcccgt cccgacgagc tggtccagcg
     4141 tgggggagcc taccttcagg tcggtcgcga agtgctcggc cgtcgcgtcg taggtgcccc
     4201 ggggatacgt gaagtgcgtg ggagcggacg cggggtcgag caggacgtac gtgacatcgc
     4261 tcagcgccac gatgacgttg gcgatccgct tgtccggaaa cttctcgaac accccgatcg
     4321 accggcccca gggccacgcc gggtccccga cgaacaccgg atctcgccac gcatgctcgg
     4381 catgaacgta gtgatgaaaa cccagcttgc cggcgtcggt gctgaagacg aagacgtcgc
     4441 cggacagggt atcgaaggcg gccgacacgc cggggaacgc gcggcccagg atggtccccg
     4501 gggcacgggg tggggcgttc accgggcggc acaggaaggt tccgcccttg gcgtcgaagc
     4561 tgaaccagct acccatgtcg gactccagat gggcgctcca ggggacgatg gcgctgacct
     4621 cgcggagcgg aatgccgatc tgcgcgggga actgtgccat cggatcggcg aacgggagct
     4681 gcggcgacgg cgagctgagc gagccaaagc ccatcagctt ccagccctgg tccgccgggc
     4741 ccggccgctg cacgtccagc ccggcggtga gctgccccac ctggtattcc cagaacagga
     4801 accggaaggt gagatagatg aagtcctgat cgctgttgcc cgccggcgcg gggttgcggg
     4861 cggtgagctg cacgtccgcc gcggcggcca ccgagaacac cgtcgggagg accgctggat
     4921 cggacgcgtt gccgggatcg agcacgaccg ttggcggcgc cacccactcc ttggtgaagt
     4981 tgtagaacga gaacttgagc gtgagcctga cccgcttcgt gtcgtccgag tcgctgctga
     5041 aggtttgact gctcccgacg gagaacgcgg tcgcgttgaa cggctggggc tcgatccaga
     5101 acaggaacag ccggttgaac gcgtacaccg gctgcacgcg gtcggagtcg atcgtgatcc
     5161 cggtcttctg ccagggctgc cagtcgatgt gatcctcgga ggtgagggtg ccgagccggt
     5221 agtaatggac cggcggctcc ttgcgcgaca cgccgatcgt gaacaccccg gtgcgctccg
     5281 cgccggcgtc atcgtggaac gtgtagcggt atccgccgac gatgcggagc tgcgcgatct
     5341 cgtggaacga ctcgaggtac cgctgatagc cgtccgtgac ggtgatcgcg gtgatctcgt
     5401 cctggagcag cttctgctcg agctcctcga acgccgggct cttcaccggc cgcagctcgg
     5461 ggcggatgta gttctcgggg tggaggaaca ccttccggtt ggcctcccag atccggtaat
     5521 tcttcatcca cggccaccac gccttgaggt tcaccctcac cgggtcgggg agatcctcga
     5581 ggtggatcaa cgcgcggtgg tagaacagct ggacgctgtt gatcgcctcg acgatcttcg
     5641 acgtcgtcga cgacgcgtcc atctggacgt cggtgagcag gtactcgtag aggtcgttgg
     5701 cgtcgcgcag gtgctcgggg ccggtgggtc ccggccgctg gaggttctcc acgaggtagg
     5761 ccaccagcgc gtcgcgcctc gcggactcca ccggatcgag cagcgaccgc gctaccccgg
     5821 gccagtcctt cgggctggtc gctaccttga tcaggccgta gaggacgtcg gccgccgcct
     5881 ggagctgagg cgccccggcg ccgaacacca ggtcccagac ctgcgacttg agcctggacg
     5941 gagtggtccc gatccgatcg gccagctcga agcagcgcgc gagccggacc acggcccgtg
     6001 catcgacgag cgcgctcggc gcgagcgcaa agatcgccag gagcgcggtg atctcgctcg
     6061 ccggccactg cgtcgcgcgc gcgagcccgg ccaccgggtc ggccggcggg atgtcgaggg
     6121 tatcgagcac cgtggtggtc tgcgacgaga agtcgtcgaa cagctgcttg atccgccacc
     6181 cgtccgggag cgcgcccagc tcgaagtcga agcggtccac gagcttgccg gcgttgtcca
     6241 gcgggtagcc cgcgtcgggc cgggtgagcc ggaagtccga gatccggaca tgctgggcgc
     6301 ccttgaacag gtaggcgcgg ccatcgaacc cggcggcggc gtccagatcg gcgcggaagg
     6361 cgtcgggcca caggccaccg atgtccgtcg cgatggcccg cggatactcc tcgtcgacgt
     6421 acgtgcggtc cgcgccggag tacaccgaga cctggggtcc ggcgaacagg tacagcttcc
     6481 cgctcggagc cacgagcgcg gcgtcgagct tcttgctggc ctggagccgg ttgtcgacca
     6541 ggccccagcg ggggaccagc ggctgggcca cgtcggggtg atcgctggtg acgacctgat
     6601 cctggacgaa gtagaacgtc gtgccgccga gggtcagcgc cgcatcgacg ccctggttca
     6661 cgatcggcgc gagcgcggtg gcgccgatct ccttggcgta cgacgacgcg atcttccgcg
     6721 gatagccctc gtcgaccgag gtgcgatcgg tcccggagta ccggacgtac tggtcgccgc
     6781 tgaacaggaa cagcttgccg ccggcgacca gcgcggcgtt gacgaactcg ttgtcccaca
     6841 gcttgttgtc gacgatgccc cagcgctcgt tcagcgggcg cagggtgccg gggctggccg
     6901 acgacacgta gagctggggc tgcgacgtat cggacggcgg cggcccgaag gcgtggaggt
     6961 tgccgtcggt cccggcgaag gcgccgcgca gcccggacgg cagcggggcc ggcagcccgg
     7021 ggaacgcgcc atcggccgcg gccaggtgag cgatgacctt gggataaccc tcgtcgacga
     7081 agccctcgta acccccgctg tagcggacgt actggtcgtc gcagaacacg agggtcaccg
     7141 cgccggcgcc ctggccgagc acgaagccgg cgtcgacgcg gttctgcgac tggctctggt
     7201 tgcggacgtt cgcccactgc gggatcaccg gcgcgggcgc cggcgactgc gagctggtga
     7261 ac
//
LOCUS       DASVOR010000749         1901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_36458,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000749 DASVOR010000000
VERSION     DASVOR010000749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36458"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF516_14785"
     CDS             <1..183
                     /locus_tag="VF516_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8108995.1"
                     /translation="TTTSAPAAAAPGNGAAAGAHAPAAGSSVGTATIARGFAWMFSGA
                     KLPAPRLLDLLLGTGA"
     gene            185..1342
                     /gene="cfa"
                     /locus_tag="VF516_14790"
     CDS             185..1342
                     /gene="cfa"
                     /locus_tag="VF516_14790"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEX8108996.1"
                     /translation="MVATATRKLLSSLDPRRAQDLFVDMLARADIQVGGSRPWDLQVH
                     DERVWTRLLRDGTLGAGEAYVDGWWDAPALDQFIDHVLRARFDESLRENWTLVAQAVR
                     ARVLNLQSITRSFDNGQHHYDIGNDLYEAMLGGRLLYTCAYWHDATTLDEAQDAKLDL
                     VCRKIGLKAGMRVLDLGCGWGGFAAFAAERYGATVTGYTVSQEQVRWAKDHYPKLPID
                     IRLDDYRNATSTFDAVVSIGLMEHVGWKNYRGYMQLVDRLLAPGGVAFVHTIAGNTPR
                     SHIEPWFDKYIFPNAVLPTLARLASAMEGILIPEDVHNIGEDYDRTLMAWWHNFDAAW
                     PTLRARYGDTFYRRWKYYLLSCAGAFRSRSQQLFQIVMTRPGTAAPPGRRG"
     gene            complement(1425..>1901)
                     /locus_tag="VF516_14795"
     CDS             complement(1425..>1901)
                     /locus_tag="VF516_14795"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEX8108997.1"
                     /translation="FTLGCTTGVIAKCYRWGYRPWVTGYGDLVAMHWACTRLARADYC
                     GDGVPHTQDGTWINLWDDLPPPGPIQQHGAKTPDGMHFEAAWNTDGAECLSRTRWKAD
                     MRSISRTCPGRRMSGGDAKKFCDTVDDAHRHGLTVLLFNESFDDPLHPKLGALVKR"
BASE COUNT          278 a          742 c          590 g          291 t
ORIGIN      
        1 acgacgacgt ccgctcccgc cgccgcagca ccgggcaacg gcgccgcggc cggggcgcac
       61 gctcccgccg ccggctcctc ggtcggcacc gccacgatcg cacggggctt tgcctggatg
      121 ttctccggtg ccaagctccc cgcgccccgt ctcctcgatc tcctccttgg cacaggagcc
      181 tgacatggtc gccaccgcca cccgcaagct gctgtccagc ctcgatcccc gccgcgccca
      241 ggacctgttc gtcgacatgc tcgccagggc cgacatccag gtcggcggca gccgtccctg
      301 ggacctccag gtccacgacg agcgcgtctg gacgcggctg ctccgcgacg gcacgctcgg
      361 cgccggcgag gcctatgtcg acggctggtg ggacgccccg gcgctcgatc agttcatcga
      421 tcacgtcctg cgcgcccggt tcgacgagag cctgcgcgag aactggaccc tggtcgccca
      481 ggccgtccgg gcccgcgtcc tcaacctgca gtcgatcacg cgctcgttcg acaacggcca
      541 gcaccactac gacatcggca acgacctcta cgaggccatg ctcggcggcc gcctgctcta
      601 cacctgcgcc tactggcacg acgccaccac gctcgacgag gcccaggacg ccaagctcga
      661 tctggtgtgc cgcaagatcg gcctcaaggc ggggatgcgc gtcctcgatc tcggctgcgg
      721 ctggggcggg ttcgccgcgt tcgccgccga gcgctacggc gccaccgtca ccggctacac
      781 ggtgtcccag gagcaggtcc gctgggccaa ggaccactac cccaagctgc cgatcgacat
      841 ccggctcgac gactaccgca acgccaccag cacgttcgac gccgtggtct cgatcggcct
      901 gatggagcac gtcggctgga agaactaccg cggctacatg cagctcgtcg atcgcctgct
      961 cgcccccggc ggcgtcgcgt tcgtccacac catcgccggc aacacgccgc gctcgcacat
     1021 cgagccgtgg ttcgacaagt acatcttccc caacgcggtg ctgccgaccc tcgcacgcct
     1081 cgccagcgcg atggagggca tcctcatccc cgaggacgtc cacaacatcg gcgaggacta
     1141 cgaccgcacc ctgatggcct ggtggcacaa cttcgacgcc gcctggccca cgctgcgcgc
     1201 ccgctacggc gacacgttct accggcgctg gaagtactac ctgctgtcgt gcgccggcgc
     1261 gttccgctcg cgcagccagc agctgttcca gatcgtcatg acccgccccg gcaccgcggc
     1321 gccccccggc cgccgcggct gaccggccgg tgccgccgga cccgacctga tacgctacgc
     1381 agggttcacc ggcacaccac caccggatgg tgtccctggc cccctcatcg tttcaccagc
     1441 gctcccagct ttggatggag cgggtcgtcg aacgactcgt tgaacagcag gacggtcagc
     1501 ccgtggcggt gcgcgtcgtc caccgtgtcg cagaacttct tcgcgtcgcc ccccgacatg
     1561 cgccgcccgg ggcatgtcct ggagatcgac ctcatgtccg ctttccagcg cgtgcggctc
     1621 agacactccg cgccgtccgt gttccatgcg gcctcgaagt gcattccgtc cggtgtcttc
     1681 gcgccgtgct gctggatcgg accgggcggc ggcaggtcgt cccagagatt gatccacgtg
     1741 ccgtcctggg tgtgcggcac gccgtcgccg cagtagtcgg cgcgcgcgag ccgcgtgcag
     1801 gcccagtgca tcgcgaccag gtcgccgtag cccgtgaccc acggtcgata accccagcgg
     1861 tagcacttcg cgatcacgcc ggtggtgcag ccgagggtga a
//
LOCUS       DASVOR010000750        12067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_95384,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000750 DASVOR010000000
VERSION     DASVOR010000750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12067
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95384"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1871
                     /locus_tag="VF516_14800"
     CDS             <1..1871
                     /locus_tag="VF516_14800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEX8108998.1"
                     /translation="VREAMHAVFLYHAIKAGLDMAIVNAGQLAVYDELDADLRGCVED
                     LVLDRRPDATERLLELAATLGGDAARQVDELAWRRQPLADRLAHALVNGITDFIDPDI
                     AEALTVYEKPLDIIEGPLMAGMNVVGELFGSGKMFLPQVVKSARVMKKAVAILEPLMD
                     AEKRRTGLASAKGKMVIATVKGDVHDIGKNIVAVVLRCNGYEVTDLGVMVPGHKILDT
                     AAELGADLVGLSGLITPSLDEMIGVASEMTRRGLTLPLLIGGATTSGKHTAVKIAPAY
                     AGPTIHVPDASLAVGVMGKLLSSERGGYVAEIRAKQASLRDAHAASQGRALLTLDQAR
                     ARRPKLVFDATTVPVPAFLGARNVSVALGELVPWIDWSPFFHTWELSGRYPAILDDPR
                     KGEAARKLFADAQALLRRIVTERLLAARGTYGLFPAGSTADDEIAVFDPADRARELVR
                     FAMPRQREDKDVCYSLADFIAPLGIAPVGGHPDHLGAFIVTAGIGTDDLAARFERDHD
                     DYSAIMTKALADRLAEAFAEYLHHRVRVDWGYGAGEALSHDDIIAENYRGIRPAFGYP
                     ACPDHAPKATLFALLDHADHHGVSLTESYAMYPTASVSGLYFAHPAATYFSVGRQR"
     gene            complement(1880..2446)
                     /locus_tag="VF516_14805"
     CDS             complement(1880..2446)
                     /locus_tag="VF516_14805"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8108999.1"
                     /translation="MNAMKHEFPPRSLDERRRTGCDRLDEVWAGVPHLVPPPSIFHQD
                     FEYALEQALRAITARRGLRTLHNIALSVPGTGWNEYRVPDLCVAHPGQLSEQALEGHA
                     ELVVEILSPRDESREKLPFYAGRGVREVWLIDPVSRDVEVLALRSRSYERVLAIDGTT
                     HAPALAIDLRVVAGPRLQLVDGAFTSEV"
     gene            2641..2865
                     /locus_tag="VF516_14810"
     CDS             2641..2865
                     /locus_tag="VF516_14810"
                     /inference="COORDINATES: protein motif:HMM:NF016028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SlyX family protein"
                     /protein_id="HEX8109000.1"
                     /translation="MSDRPETAPDADAERWLDLDVKLAFQERLIRELDALVREFGDRL
                     AKAERELAELRQAMPPPVPLGPINEPPPHY"
     gene            2962..3732
                     /locus_tag="VF516_14815"
     CDS             2962..3732
                     /locus_tag="VF516_14815"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX8109001.1"
                     /translation="MHRRLVVLALTAGCGSVQNSTPDGMPPDGSVDLARGCALEAQMD
                     EASWLTGSPVVNSCGGSGGQLTGMTAMPVSDAIRGRVGSFSGNACVNFTSTAALHGTT
                     GLTMSAWVKPTGLNGVDSNGVITKRVDRGVQSEYSLNVWTGYHVWVDFGDVDRYSGTA
                     TLSNNVWVQLTAVFDGTRAPADRVRLFINGVPDPLQHAVIGNLGTTLPSYDSPVHVGC
                     TPAPMAMTQQTFIGELDNVTIWNRALSDAEIAQLYANG"
     gene            3844..4146
                     /locus_tag="VF516_14820"
     CDS             3844..4146
                     /locus_tag="VF516_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109002.1"
                     /translation="MQKLRADSGHPMQHALQREQARERLAALSPGGSPQRAIPVASAA
                     VIEVRAAVMPCPQCGGLYRLHEHTRPLPGVRRIDVACRQCSTPRALWFRIVDDEPN"
     gene            4187..4492
                     /locus_tag="VF516_14825"
     CDS             4187..4492
                     /locus_tag="VF516_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109003.1"
                     /translation="MIRRKRTEQRERARAMRKLVRDREKLAALSAGGSEQRPIHVTSA
                     AVVEVRIGHLSCPQCGGEYRIKEHRAPASGLRSVDVTCQICGVPRTLWFRIGSAEAN"
     gene            4876..5430
                     /locus_tag="VF516_14830"
     CDS             4876..5430
                     /locus_tag="VF516_14830"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8109004.1"
                     /translation="MRIFISYSSTDAAHAAAISARLPPEYRVFFDDHALAPGHAYDEK
                     IAREIAECDLFVFLLSPAALEPGGYALTELKIFQKRWRNPQGRLLVVMVRDVNLRSDD
                     PVLAYLTAVTVLRPLGNVAAEVVREIAQLSVPRPSGQRPPTCPEMQIVHELLQARDFQ
                     ARREVGELVTWWRPLRSGSCARGV"
     gene            5746..7341
                     /locus_tag="VF516_14835"
     CDS             5746..7341
                     /locus_tag="VF516_14835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109005.1"
                     /translation="MRLVEGAEDGRYTLHPAVRDGFLRGMAITNSRDGNRAVCDTLLA
                     GLRTGPGAPPPTELTTLDEIEELIHHTLELDDVNEAWQLFEYRMGGFENLGWRLGAFE
                     RIDRILRRIAQHVPAPGASALGLVARRALEHAWARSLAARGEIAQAIARCTVVRDQGL
                     YDEALIELDMLAELYVLSGRLRHALECVEEALTHAKHQGDHEAKARTLGLLVHIQALR
                     GKRLAAKQAAHGQAQRELDEPFFHVRRLPAMHHLTRLGELDEAARILDRAEQVDRASW
                     GGDGPAIQALALERAEIALAHGDHDRIEPRVRAVREWATQHDAKELLCWSGRLLIEQR
                     LQAWHTGTAVEPAELRAVLQLAMVEVDRARSNGLGLYHIDLRIQRARLRLLTGDPLAA
                     IADLAIAFEDGLPADDSGPDVPAARQPSCKYAWGVADARRCLGHALLLYVAIDLGDAK
                     GDPRTLLERPEVRAAIRELESARNVMHTMGDPRADEIERELEAVARGLLTASPLDRVV
                     EPLAEADRLRATRVITAALAAAR"
     gene            7605..8936
                     /locus_tag="VF516_14840"
     CDS             7605..8936
                     /locus_tag="VF516_14840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109006.1"
                     /translation="MAAKTPAGAGGAIDEHRLVAPGDQDALAILAEHHGPPSAEAVGQ
                     HARRVGAVQRPQQDGPVGQSHHLVPIRAERRGARLVEVRAIGACIEHDGLAGPVGIPD
                     LRGFPLVDHQDLRRVRAERHVARNPHICPLGPRDHDRGTGPVGSVHACAAIADGDQDA
                     LPIEAEHGLVEKRTFVDAAEHDRCPLAIGVPHARGTVARCRDDAHPVRAEVRGPDLGA
                     VATEDDGGALAIRGPDPGRSVRGRGDDALTGAVELRREYRARVSSQKNPRLTATLRVE
                     HPHRAVAACGDHLGAIRAETRALDPPVGVLADQPARVTDEERARHELIQQRARRVHAT
                     ARLGRAVELGEHQRGGQRVILRHAARHRHQAGGPRRARGLRQVLRGFPGLRRAPQGVA
                     AESYDDRHDRRGSRERDEPPAPCGRQARPDEALQRRRQAREQAGIQHLRLA"
     gene            complement(8998..10395)
                     /locus_tag="VF516_14845"
     CDS             complement(8998..10395)
                     /locus_tag="VF516_14845"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8109007.1"
                     /translation="MSAVFISHSSADALVAAEIAELLRQRGHRSIFLDFDPALGFPAG
                     RSWEKELYAQLRACRVVIVLCSAASMASDWCFAEITHARALGKHVIPVKIAPCVMRPL
                     LMDVQIVDLTVDRENDLGQLWRGLEDAGLDPAGTFERDPERPPYPGLLAFEEQDAAVL
                     FGRDAEIRAGLDVLRRQHRFDGARFVVFLGASGSGKSSLVRAGLVPRLRRDGAHWQVL
                     GVMRPSDAWPSMLAALFTDAFARCGSAVDRTSLLADFEDADRRVAALLARCRELRVAA
                     RALTASVVLVLDQLEEAFDVRHQADVAALWSMVGAALAAPRSPLLVAATLRSDFFGVF
                     QTHASVMALAYEQVPVDAIPVSRFPELIEGPAQLAGLELAVGLTGALVQDTAAADALP
                     LLAFALRELWERRSRQRIDLATYRNELGGLAGSVARAAEAVLQVHRPSSAELAALLGP
                     AGRLARRRPGVPAVA"
     gene            complement(10429..10683)
                     /locus_tag="VF516_14850"
     CDS             complement(10429..10683)
                     /locus_tag="VF516_14850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109008.1"
                     /translation="MAIDDLDTLEHKLGKRGFRRIDGHLYECTACHARAVRKYATLGR
                     TGGRDISLCEACGVATSWRSVAGMEQREQDVGFDLRAFLG"
     gene            complement(10707..11465)
                     /locus_tag="VF516_14855"
     CDS             complement(10707..11465)
                     /locus_tag="VF516_14855"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8109009.1"
                     /translation="MTKPDVRSQVGEPEWQLRVDLAACYRLVAHFGWSDLIFTHISAR
                     LPGPEHHFLINPYGMTFDEITASSLVKIDLEGQKVMDSPYEINPAGFTIHSAIHAARD
                     DARCVLHTHSVNGVAVSAQKCGVLPISQQSTFVLSSLAYHDYEGVALNADEKPRLVRD
                     LGDKVFLMLRNHGLLTVGPTVADAFVAMYTFEATCMIQIRAQAGGELVRVDPRIIEGA
                     MAARQTVTQGVGPGALAWPGLLRMLDRIDPSFRS"
     gene            11564..>12067
                     /locus_tag="VF516_14860"
     CDS             11564..>12067
                     /locus_tag="VF516_14860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109010.1"
                     /translation="MPDRPMHTVPGLFDRLTALYADGMRASIEGRLHDAVARFSEGIQ
                     LDDQFLQGSVTLYAQRAFVYQRLGDNVAAIRDYGRAIEMEPPANQAQYLFHRGMCFAA
                     LGGHEEHAVNDFGRAIALSPDQPGPYHLRGKLYATDLGRYPEAIADFDCLLAMHPVAE
                     AYQLRGYA"
BASE COUNT         1862 a         4365 c         4081 g         1759 t
ORIGIN      
        1 gggtccgcga ggcgatgcac gcggtgttcc tctaccacgc gatcaaggcc gggctcgaca
       61 tggcgatcgt caacgccggc cagctcgcgg tctacgacga gctcgatgcg gatctgcgcg
      121 ggtgcgtcga ggacctcgtg ctcgaccggc ggccggacgc caccgagcgg ctgctcgagc
      181 tcgccgcgac gctgggcggc gacgcggccc gccaggtcga cgagctggcg tggcgccgcc
      241 agccgctggc cgaccggctc gcccacgcgc tggtcaacgg catcaccgac ttcatcgatc
      301 cggacatcgc cgaggcgctg accgtgtacg aaaagccgct cgacatcatc gagggcccgc
      361 tgatggccgg gatgaacgtc gtcggcgagc tgttcggctc gggcaagatg ttcctgcccc
      421 aggtcgtcaa gtcggcgcgc gtgatgaaga aggcggtcgc gatcctcgag ccgctgatgg
      481 acgccgagaa gcggcgcacc gggctcgcct ccgccaaggg caagatggtg atcgccacgg
      541 tcaagggcga cgtccacgac atcggcaaga acatcgtcgc ggtggtgctg cgctgcaacg
      601 gctacgaggt caccgacctc ggggtcatgg tgcccggcca caagatcctc gacaccgcgg
      661 ccgagctcgg cgccgacctc gtcgggctgt ccggcctgat cacgccgtcg ctcgacgaga
      721 tgatcggcgt ggcgtccgag atgacccggc gcggcctgac gctgccgctg ttgatcggcg
      781 gcgcgaccac gagcggcaag cacaccgcgg tcaagatcgc accggcctac gccggaccga
      841 cgatccacgt ccccgacgcg tcgctcgcgg tcggcgtgat gggcaagctg ctgtcgagcg
      901 agcgcggcgg ctacgtcgcc gagatccgcg ccaagcaggc ctcgctgcgc gacgcccacg
      961 ccgcgtcgca gggccgcgcg ctgctgacgc tcgaccaggc gcgcgcccgc cggccgaagc
     1021 tcgtgttcga cgccaccacc gtgccggtcc ccgcgttcct cggcgcccgc aacgtcagcg
     1081 tcgcgctcgg cgagctcgtg ccgtggatcg actggtcgcc gttcttccac acctgggagc
     1141 tgtccggccg ctacccggcg atcctcgacg acccccggaa gggcgaggcc gcgcgcaagc
     1201 tgttcgccga cgcccaggcg ctgctccgcc ggatcgtcac cgagcggctg ctcgccgcgc
     1261 gcggcaccta cgggctgttc ccggcggggt cgaccgccga cgacgagatc gccgtgttcg
     1321 atcccgccga tcgcgcgcgc gagctcgtcc ggttcgccat gccgcgccag cgcgaggaca
     1381 aggacgtgtg ctactcgctc gcggatttca ttgctccgct tggcattgct ccggtcggcg
     1441 gccaccctga tcacctcggc gcattcatcg tcaccgccgg gatcggcacc gacgatctcg
     1501 ccgcccggtt cgagcgcgac catgacgact actcggcgat catgaccaag gcgctcgccg
     1561 accggctcgc cgaggcgttc gccgagtacc tccaccaccg ggtccgcgtc gactggggct
     1621 acggcgccgg cgaggcgctg tcccacgacg acatcatcgc cgagaactac cgcggcatcc
     1681 gccccgcgtt cggctacccc gcgtgcccgg accacgcccc caaggccacg ctgttcgcgc
     1741 tgctcgacca tgccgatcac cacggcgtgt cgctgaccga gtcgtacgcg atgtacccga
     1801 ccgcctcggt gtccgggctg tacttcgccc acccggccgc cacgtacttc tcggtcggcc
     1861 gccagcgctg acccgcgcgc tatacctcgc tggtgaaggc gccgtcgacc agctgcagcc
     1921 gcggcccggc gaccacgcgg agatcgatcg ccagtgccgg ggcatgggtc gtgccatcga
     1981 tcgccaggac gcgttcgtag ctgcggctgc gcagcgcgag cacctcgacg tcacgcgaga
     2041 cagggtcgat caaccacacc tcacggacgc cacggccggc gtagaatggc agcttttcgc
     2101 ggctctcgtc gcgaggcgac aggatctcga ccaccagctc cgcgtggcct tcgagcgcct
     2161 gctccgacag ctgccccggg tgcgcaacgc acaggtcagg gacccggtat tcgttccagc
     2221 cggtgccggg aacggagagg gcgatgttgt gcagcgtgcg gagcccgcgc cgcgcggtga
     2281 tcgcacgcag cgcctgctcc agcgcgtatt cgaagtcctg atggaagatg gaaggtggcg
     2341 gcacgaggtg tggaactccc gcccagactt catccagccg atcgcacccg gtccggcgtc
     2401 gctcgtcgag cgacctcggc gggaactcgt gcttcatcgc gttcatgcga ggagtgtagc
     2461 ctggaagcat tccggaaccc cttagcaagc agtgcgcccg tgcagatccc cgagatctca
     2521 tcggcaggtg tccggagcgt ccggccggcg gccggggttg tccggaatcc ggccaaatgc
     2581 cggacagcgc gagcgacgcg tccggcgccc ggacagctcg atcgcggcta tcgtagcgcc
     2641 atgtccgacc gccccgagac cgcccccgac gccgacgccg agcgctggct cgatctcgac
     2701 gtcaagctcg ccttccagga gcgcctgatc cgcgagctcg acgccctggt ccgcgagttc
     2761 ggcgaccggc tcgccaaggc cgagcgcgag ctcgccgagc tgcggcaagc catgccgccg
     2821 ccggtgccgc tcggcccgat caacgagccg ccgccgcact actgaaccgg cattgcactc
     2881 gatgtgcggc agaccacggc acgcggcacg gcgagccgtc gatcgtgtct cggcagcgcg
     2941 cgggtcgggt agcatctctc catgcatcgc cggctcgtgg tcctggcgct caccgcgggc
     3001 tgcggcagcg tccagaattc aactcccgac ggcatgccgc ccgacgggtc ggtcgatctg
     3061 gcgcgcggct gcgcgctcga ggcccagatg gacgaggcgt cgtggctgac cggcagcccg
     3121 gtcgtcaaca gctgcggcgg cagcggcggc cagctcaccg gcatgaccgc gatgccggtg
     3181 tccgacgcca tccgcggccg ggtcggcagc ttctcgggca acgcgtgcgt caacttcacg
     3241 agcaccgccg cgctccacgg caccaccggc ctgacgatga gcgcatgggt caagccaacc
     3301 ggcctcaacg gggtggactc caacggcgtc atcaccaagc gggtcgaccg cggcgtgcag
     3361 tccgagtact cgctgaatgt gtggaccggc taccacgtgt gggtcgactt cggcgacgtg
     3421 gaccgctatt ccggcaccgc gaccctgtcg aacaacgtct gggtccagct caccgccgtg
     3481 ttcgacggca cgcgggcgcc ggccgaccgg gtccggctgt tcatcaacgg cgtgcccgac
     3541 ccactgcagc acgccgtcat cggtaacctc ggcaccaccc tgccgagcta cgactcgccg
     3601 gtccacgtcg gctgcacgcc ggcgccgatg gccatgaccc agcagacctt catcggcgag
     3661 ctcgacaacg tcacgatctg gaatcgcgcg ctcagcgacg ccgagatcgc gcagctctat
     3721 gccaatggct agcgtcgatc ctgtccggcc tcgatgggcg caccgagcgg ccgcgtaccc
     3781 agcgcgcggc aactgaccga tcggtcagct gctcgcgcgc cgcgcctgtc ctacgctgag
     3841 aaggtgcaga agctccgcgc cgactccggg catccgatgc agcacgccct gcagcgcgag
     3901 caggcgcgag agcggctcgc ggcgctgtcg ccgggtggct ccccgcagcg cgcgatcccg
     3961 gtcgcgtcgg cggcggtgat cgaggtccgc gcggcggtga tgccgtgccc gcagtgcggc
     4021 ggcctgtacc ggctgcacga gcacacgcgc ccgctgcccg gcgtccgccg gatcgacgtc
     4081 gcatgtcggc agtgcagcac gccgcgcgcg ctgtggttcc ggatcgtcga cgacgagccc
     4141 aactgacgag gcggcgcccg gcggtccgtg ctacacgaac aggcctgtga tccgccgcaa
     4201 acgcaccgag cagcgcgagc gtgcccgcgc gatgcgcaag ctggttcgcg atcgcgagaa
     4261 gctcgccgcg ctgtcggccg gcggctccga gcagcggccg atccacgtga cctcggccgc
     4321 cgtggtcgag gtccggatcg gtcacctgag ctgcccgcag tgcggcggcg agtaccggat
     4381 caaggagcac cgcgcgccgg cgtccgggct gcgcagcgtc gacgtgacct gccagatctg
     4441 cggcgtgccg cgcacgctgt ggttccggat cggcagcgcc gaggccaact gagccgggat
     4501 gccgccgaac gtcaagctcc aagtatcctt gactacttgg atatttccgt cggggtggca
     4561 ggcccctggc ttcatccgtg acagggcttc gtccgctata ggcgatgatg gccacgcaga
     4621 ccgaggcgct cccgctcgca tcgatcgcat cggcccgggc gccagcgagc cggtgcgcgt
     4681 ttgcgaaccg acgaatcccc gggcccgagg ttgaccgcgg agcagaacct gcggctcgcg
     4741 gtgcaatcgg gattacgcca cggcggcgac gacttctcga ccgacccgtg gcggagcctc
     4801 cagcccctgg gctcagcacg ggcgagtcgc gtcgtgggga gtgttaccat ggtaggtggg
     4861 gcgacgcaga tcttcgtgag gatcttcatc tcgtactcga gcaccgatgc cgcacacgca
     4921 gccgcgatct ccgcacggct gccaccggag tacagggtgt tcttcgacga ccacgcgctg
     4981 gcgccgggac acgcctacga cgagaagatc gcgcgcgaga tcgcggagtg cgacctgttc
     5041 gtgttcctgc tcagtcccgc ggcgctggag ccgggtggct atgcgctgac cgagctgaag
     5101 atcttccaga agcgctggcg taacccgcag ggtcgcctgc tcgtcgtcat ggttcgcgac
     5161 gtgaacctcc ggtccgacga tccggtcctc gcatacctga ccgccgtcac ggtgctgcgt
     5221 ccgctgggga acgttgccgc cgaggtggtt cgggagatcg cgcaactctc ggtgcccagg
     5281 ccatctggcc aacgcccgcc cacctgccca gagatgcaga tcgtgcatga gctgctgcag
     5341 gcgcgcgatt tccaggcgcg ccgcgaggtc ggcgagctcg tcacgtggtg gcggccgttg
     5401 cgctccggct cctgcgcgcg cggggtatag cgacgggctc ggatgccgcg ctcgccgccc
     5461 tcgccagcga acacggcaac cacgcgctca gcgtcgatct ccttggtggg ctcatcgcac
     5521 gctactacgg tggtgatccg gctcggcttc ctgcggttcc ctcggtgcca cagcagaacg
     5581 tcgcgggtgt cgcggatccc gatgatgagg agctgcagga gcaggagcgc aggttctcgc
     5641 gaatcgcaat gcgatatcgc actctgttct gcgagcggga tccggattca ctcgccgtgc
     5701 tcgagcacat ttgcctgttc cgtaccggtg tgtctgctct cgttgatgcg gctcgtggag
     5761 ggtgcggagg acggaaggta cacgcttcat cccgcggttc gagatggctt tcttcgcggc
     5821 atggccatta cgaactcgcg cgacggtaat cgcgccgtat gcgatacact gctcgccggc
     5881 ctgcgcaccg gcccgggcgc tccgccgccc acggagctca cgacgctcga cgagatcgag
     5941 gagctcatcc atcacacact cgagctcgat gacgtcaatg aggcatggca gctgttcgag
     6001 taccgcatgg gcgggttcga gaacctcggc tggcgcctcg gagccttcga gcgcatcgac
     6061 cggatcttgc gccggatcgc gcagcacgtg ccagcgcctg gcgcgagcgc cctcggcctt
     6121 gttgcgcggc gcgcgctcga acatgcatgg gcgcgaagcc tggcggcgcg cggcgagatc
     6181 gcgcaagcca tcgcgcgctg caccgtggtc cgcgaccagg gcctgtacga cgaagcgttg
     6241 atcgagctcg acatgctggc cgaactgtac gtgttgtccg gtcgtctccg gcatgcgctc
     6301 gaatgcgtcg aggaggcgct gacacatgcg aagcatcagg gcgaccatga ggccaaggcc
     6361 cggacgctcg gcttgctcgt ccacatccag gcgctgcgcg gcaagcggct cgcggccaaa
     6421 caggccgcgc acggtcaagc tcagcgagag ctcgacgagc cgttctttca tgtgaggcgc
     6481 ttgcctgcga tgcaccacct cacacggctc ggcgaacttg acgaggcagc gcggatcctc
     6541 gatcgcgccg agcaggtcga ccgcgcctcg tggggcggcg acggtccagc gatccaggca
     6601 ctggcgctcg agcgcgccga gatcgcgctc gcgcatggcg accacgaccg cattgagccg
     6661 cgcgtccggg cagtccgcga gtgggcgacc caacacgacg ccaaggaatt actctgctgg
     6721 tcggggcgct tgctcatcga gcagcggctt caggcgtggc acacgggcac ggcggtcgag
     6781 ccggccgagc tacgcgcggt gctgcagctc gcgatggtcg aggtcgaccg ggcgcgctcg
     6841 aacggcctgg gtctctatca catcgacctc cggatccagc gcgcccgcct ccggttgttg
     6901 actggtgatc cgctggccgc gatcgcggac ctggcaatcg cattcgagga tggcctacct
     6961 gccgacgaca gcggtccaga cgttcccgcg gcgcggcagc caagctgcaa gtacgcatgg
     7021 ggagtagcgg atgctcgacg ctgccttggc catgcattgc ttctgtacgt cgcgatcgac
     7081 ctcggcgacg ccaaaggcga tccccgcacg ctactcgagc gccccgaggt tcgcgcggcc
     7141 attcgagagc tcgagagcgc tcgcaacgtc atgcatacga tgggcgatcc gcgcgcggat
     7201 gagatcgaac gggagctcga ggcagtggca cgcggcttgt tgacggcatc accgctcgat
     7261 cgtgtcgtcg agccccttgc cgaggcggac cgactccgcg ccacgcgggt catcacggca
     7321 gcgctcgccg ctgccaggta gccggtcgag agaatcacga ggcgcgtcct tctgcatgac
     7381 ggagcacgag cagctaccct gatggcggcg aacgtccatg atctcgctcg cagcgactcg
     7441 aaaccgggca tgaacgccgc ttgctcgacc cagtcgcgat gtccgaccag ctccacgggc
     7501 tcgccggcgc ccgccgcgac ccatacgcgc gcggtgccat cggctgatgc ggtcaggatc
     7561 gcgcgctcgt cggggctgaa cacggcggac gtgacccagt tctggtggcc gcgaagacgc
     7621 cggcgggtgc cggtggggcg atcgatgaac atcgcctcgt tgccccaggc gaccaggacg
     7681 cgctggccat ccttgctgaa caccacggcc cgccctccgc cgaggccgtc ggccagcacg
     7741 cgcggcgcgt cggggcggtc cagcgtccac agcaagacgg ccccgtcgga cagagtcacc
     7801 acctcgtgcc catccgggct gaacgtcgcg gcgctcgtct tgtcgaagtg cgcgcgatcg
     7861 gagcgtgcat cgagcacgat gggctggccg gtcccgtcgg cattccagat ctgcgcgggt
     7921 tccccctcgt cgaccaccag gacctgcgtc gcgtccgggc tgaacgacac gtcgctcgca
     7981 atccgcatat ctgtcccttg gggccgcgcg accacgaccg gggtaccggt cccgtcggct
     8041 cggtacacgc gtgcgctgcc atcgccgacg gcgaccagga cgcgctgccc atcgaggctg
     8101 aacacggcct cgtcgagaaa cgtaccttcg tcgacgctgc cgagcacgac cggtgtcccc
     8161 tggccatcgg cgttccacac gcgcgcggta ccgtcgctcg atgccgtgac gatgcgcatc
     8221 ccgtccgggc tgaagtgcgc ggtccggacc tcggcgccgt ggccacggaa gacgatgggg
     8281 gtgccctcgc catccgcggc ccagacccgg gccgttctgt ccgcggacgc ggtgacgatg
     8341 cgcttaccgg agcggtcgaa ctccgccgtg aataccgcgc gcgcgtgtcc tcgcagaaga
     8401 atccgcgcct caccgccacg ctgcgcgtcg aacacccgca ccgtgccgtc gccgcctgcg
     8461 gtgaccacct gggtgccatc cgggctgaaa cgcgcgcgct cgatcccccc gttggggtcc
     8521 tggccgatca gccagcccgt gtgaccgacg aggagcgcgc gcgtcacgaa ctgattcagc
     8581 agcgcgcgcg ccgtgttcac gccaccgctc ggctcgggcg tgccgtcgag ctcggcgagc
     8641 atcagcgagg cggtcaacgg gtcatcctcc gtcatgccgc gcgccatcgc caccaggccg
     8701 gaggcccacg ccgcgcgcgt ggtctgcgcc aggtgctccg cggcttcccg ggcctgcgtc
     8761 gcgcgccgca aggcgtagcc gccgagagct acgatgatcg ccacgatcgc cgcggcagcc
     8821 gtgagcgcga tgagccgccg gcgccgtgcg gccgccaggc tcgtccggac gaagcgctgc
     8881 agcgccggcg tcaagcgcgc gagcaggccg gtattcagca cctgcgcctg gcgtagcagc
     8941 tccccgatca gcggctcacg cccttgcgcg ctatcttcgt aggcgatctc gagtcgctta
     9001 cgccacagca ggaacacctg gtcgtcggcg agccagccgg ccagccgggc ccagcagcgc
     9061 cgccagctcg gcgctcgacg ggcggtggac ctgcagcacg gcttccgcgg cacgcgccac
     9121 cgaaccggcc agccccccga gctcgttgcg atacgtcgcg agatcgatcc gctggcgcga
     9181 gcgacgctcc cacagctctc gcagcgcgaa cgccagcagg ggcagcgcgt cggcggctgc
     9241 cgtgtcctgc acgagcgccc cagtcaaccc cacagccagc tcgagtcccg ccagctgcgc
     9301 cggcccctcg atcaactcgg ggaagcggga aaccgggatc gcatcgaccg gcacctgctc
     9361 gtacgcaagg gccatgacgg acgcgtgggt ctggaacacg ccgaagaagt cggagcgcag
     9421 cgtggctgcc acgagcaggg ggctccgggg tgccgcgagg gcagctccaa ccatggacca
     9481 cagggcggca acgtcggcct ggtgtcgcac atcgaacgct tcttcgagct ggtcgagcac
     9541 gagcacgacg ctcgcggtga gcgcgcgggc ggcgacacgc agctcgcgac agcgcgcgag
     9601 aagcgccgcc acccggcggt ctgcgtcctc gaagtcggcc agcagcgatg tccgatcgac
     9661 ggcggagcca caccgcgcga acgcatccgt gaacagcgcc gccagcatcg atggccacgc
     9721 gtcggacggc cgcatcacac cgaggacctg ccagtgcgcg ccatcgcggc gcagacgtgg
     9781 cacgaggccg gccctcacca gcgacgactt tccactcccc gacgcgccca ggaacacgac
     9841 gaaccgcgca ccatcgaagc gatgctgccg ccggagcacg tcgagtcccg cgcggatctc
     9901 ggcgtcgcgt ccgaacagca ccgcggcatc ctgctcctcg aacgcgagca agccagggta
     9961 cggcggccgt tccggatccc gctcgaaggt ccccgccgga tcgagcccgg cgtcctcgag
    10021 gccgcgccac agctgcccga ggtcgttctc tcggtcgacg gtgagatcga cgatctgcac
    10081 gtccatgagg agcggcctca tgacgcacgg cgcgatcttc accgggatca cgtgcttgcc
    10141 aagtgcgcgt gcgtgcgtga tctcggcgaa gcaccagtcc gatgccatcg acgctgcgct
    10201 gcacaggacg atcaccacac ggcacgcgcg cagctgggcg tagagttcct tctcccagct
    10261 ccgaccagca ggaaacccga gtgccggatc gaagtccagg aagatcgagc gatggccgcg
    10321 ctgccgcagc aactcggcga tctcggccgc aaccagcgcg tcggcgctgc tatggctgat
    10381 gaataccgcg ctcatgagat gatcgggtcg gcgcgacccg ccaggcacct agccgaggaa
    10441 cgcgcgcaga tcgaacccga cgtcctgctc gcgctgctcc atcccggcga cgctgcgcca
    10501 cgacgtggcg accccgcagg cctcgcacag cgagatatcg cgcccgccgg ttcgcccgag
    10561 cgtggcgtac ttgcgcaccg cgcgcgcgtg gcacgcggtg cactcgtaca ggtgtccgtc
    10621 gatgcggcgg aacccgcgct tgccgagctt gtgctcgagg gtgtccagat cgtcgatcgc
    10681 catgccgcag cgtaccgcgc gcgcggctac gaccggaagc tgggatcgat gcggtcgagc
    10741 atgcgcagaa ggcccggcca ggccagcgcg ccggggccga cgccctgggt cacggtctgg
    10801 cgcgccgcca tcgcaccctc gatgatcctc gggtcgaccc ggaccagctc gccgccggcc
    10861 tgggcccgga tctggatcat gcaggtcgcc tcgaacgtgt acatcgccac gaacgcgtcg
    10921 gcgaccgtcg ggccgaccgt cagcagaccg tggttgcgca gcatcaggaa gaccttgtcg
    10981 ccgaggtcgc ggaccagccg cggcttctcg tcggcgttga gcgcgacgcc ctcgtagtcg
    11041 tggtacgcca gcgacgacag cacgaacgtc gactgctgcg agatcggcaa gacgccgcac
    11101 ttctgggccg acaccgccac cccgttgacg ctgtgggtgt gcagcacgca cctcgcgtcg
    11161 tcgcgcgcgg cgtggatcgc gctgtggatc gtgaagcccg ccgggttgat ctcgtacggg
    11221 ctgtccatca ccttctggcc ctcgaggtcg atcttgacca ggctcgacgc ggtgatctcg
    11281 tcgaacgtca tcccgtacgg gttgatcagg aagtggtgct ccggcccggg cagccgcgcc
    11341 gagatatggg tgaagatcag atcggaccag ccgaaatgcg ccaccagccg gtagcacgcg
    11401 gcgaggtcca cgcgcagctg ccactcgggt tctccgacct gcgatcgcac atccggcttg
    11461 gtcatgcgac gatggtagcg caagatcgcc gcgcggcggc aggagcgcga accgccgcgg
    11521 cccgcgcttt ccttcggcgt cggactgagc gatcgttccg gcgatgcccg atcgcccgat
    11581 gcacaccgtg cccggcctgt tcgaccgcct gaccgcgctg tacgcggacg gcatgcgagc
    11641 gagcatcgag ggccgactgc acgacgcggt cgcgcggttc agcgagggca tccagctcga
    11701 cgaccagttc ctccagggca gcgtcaccct gtacgcgcag cgcgcgttcg tctaccagcg
    11761 gctcggcgac aacgtcgcgg cgatccgcga ctacggccgg gcgatcgaga tggagccgcc
    11821 ggccaaccag gcccagtacc tgttccaccg cggcatgtgc ttcgccgcgc tcggcggcca
    11881 cgaggagcac gcggtcaacg acttcggccg ggcgatcgcg ctgtctcccg atcaacccgg
    11941 cccctaccac ctgcgcggca agctctacgc caccgacctc ggccgctacc ccgaggcgat
    12001 cgccgacttc gactgcctgc tcgccatgca cccggtcgcc gaggcctacc agctgcgcgg
    12061 ctacgcc
//
LOCUS       DASVOR010000751         3359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_65596,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000751 DASVOR010000000
VERSION     DASVOR010000751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65596"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1341)
                     /locus_tag="VF516_14865"
     CDS             complement(<1..1341)
                     /locus_tag="VF516_14865"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8109011.1"
                     /translation="MGMLSPEELARLTSAESLFASPIPVQSVSSDEFMPAPQTPRQRE
                     FESRVKQIGSTMARRLGMTRRRFFQTAAGMAAAFVAMNDTYGPLFGVSRAEAATPDQA
                     DARAKKLAGQFIMDMHTHFLRPGTRIQTFVEQRKAVGKAGWNPALGGKEQTIDDLMFA
                     NYLKEIYLDSDTKVACISGAPSEIPDDWFLTNEMKAQARADVNGLSGSKRMLAHAIFT
                     PGFPGWMDAIDHAIEALKPDSMKGYTIGDNTNKHLSKHPWRMDDEKLVYPAYEKFLKA
                     GLKNVCVHKGLFPPEVEKQFPHLLEYSDVRDVGKAAKDWPQLNFIIYHGGYRFAGGGK
                     VEDAWAQFEQTGRIDWVTDLAEIPAKYGVKNVYADVGQLFAQSTIADPRVSAVMMGQM
                     IKGLGVDHVCWGTDAIWTGSPQWQIEALRRLEIPEPLQKKYGLAPLGPADGPVKN"
     gene            1683..>3359
                     /locus_tag="VF516_14870"
     CDS             1683..>3359
                     /locus_tag="VF516_14870"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8109012.1"
                     /translation="MGAQLDFRGLVDAATDVIAVFDRDRRYVYINAALERITGVPGGS
                     VLGKRNDEVMPADDALVWTEALDDVSRSGRPRAIESTLATPHGPRRFASVLTTVSDGL
                     VCAVSRDITEIGRRYDEVDLDMFTELARRTGVALDNARLLAAEQEARRHAEEARDRTR
                     RLQQLTAALSGALDEHDVVSIMVNAGRDAVGAGAGFAWLLRGGDTLELAGSAHDDRPI
                     RLDSFQAIAMTAPLPVYDVVRSGQPMLFESLAALKASYPQAAPASDLPFRSWAVIPIV
                     LAGRGAGAVSFSFAEERTFRDDDRELLTAMAGQASLALERARLYDALQNTAQQLATAN
                     RRKDEFLAMLGHELRNPLAPIATALDVMEIKDPSAMRLEREVIRRQVDHLSRLIGDLL
                     DVSRITRGKIQLTREVLELGTVLAAAMEMAAPMLERRMQQLAVDVPYGGLLVDADPTR
                     LAQVFQNLLINAAKYSEPRSEITLRARGRSDHIVVEVVDQGMGISPDLLPRLFDLFVQ
                     GERALDRSQGGLGIGLTIARSLCELHGGTITAASAGPGRGSTFTVTLPRAE"
BASE COUNT          459 a         1164 c         1204 g          532 t
ORIGIN      
        1 gttcttgacc gggccatcgg cgggcccgag cggcgccagg ccgtacttct tctgcagcgg
       61 ctcggggatc tcgagccggc gcagcgcctc gatctgccac tgcggcgagc cggtccagat
      121 cgcgtcggtg ccccagcaca cgtggtcgac gccgaggccc ttgatcatct ggcccatcat
      181 cacggcggac acccgcgggt cggcgatcgt gctctgcgcg aacagctgcc cgacgtcggc
      241 gtagacgttc ttcacgccgt acttggccgg gatctccgcg aggtcggtga cccagtcgat
      301 ccggccggtc tgctcgaact gcgcccacgc gtcctcgacc ttgccgccgc ccgcgaagcg
      361 gtagccgccg tgatagatga tgaagttgag ctgcggccag tccttggcgg ccttgccgac
      421 gtcgcgcacg tcggagtatt cgagcaggtg gggaaactgc ttctcgacct cgggcggaaa
      481 caggcccttg tggacacaga cgttcttcag gcccgccttg aggaacttct cgtacgccgg
      541 atagaccagc ttctcgtcgt ccatccgcca cggatgcttg gacaggtgct tgttggtgtt
      601 gtcgccgatc gtgtagccct tcatcgagtc gggcttgagc gcctcgatcg cgtggtcgat
      661 cgcgtccatc cagcccggaa agccgggagt gaagatcgcg tgggccagca tccgcttcga
      721 gccggacagc ccgttgacgt cggcgcgcgc ctgcgccttc atctcgttgg tgaggaacca
      781 gtcgtcgggg atctccgacg gcgcgccgct gatgcacgcg accttggtgt cgctgtcgag
      841 gtagatctcc ttgagatagt tcgcgaacat caggtcgtcg atcgtctgct ccttgccgcc
      901 gagcgccggg ttccagcccg ccttgccgac cgccttgcgc tgctcgacga aggtctggat
      961 ccgcgtgccc ggccgcagga agtgggtgtg catgtccatg atgaactggc cggcgagctt
     1021 cttggcgcgc gcgtcggcct ggtcgggcgt ggcggcctcg gcgcgcgaca cgccgaacag
     1081 cgggccgtag gtgtcgttca tcgcgacgaa cgccgccgcc atccccgccg cggtctggaa
     1141 gaaccgccgc cgggtcatgc ccagccggcg cgccatcgtc gagccgatct gcttgacgcg
     1201 cgactcgaac tcgcgctgcc gcggggtctg cggcgcgggc atgaactcgt cgctcgacac
     1261 cgactgcacg gggatcgggg atgcgaacag cgactcggcg ctggtcaacc tggcgagctc
     1321 ttccggggac agcataccca tgggccgatc ctcctcgtgc gtgggtcccg atccgtatcg
     1381 agcggccgcg ccgccgtcaa cgccgtcgat cggggcgatg ggtggggcga tgtgcctcgc
     1441 ctgcaggtcc gcgccggcag tctatcggcc ggcggtggcc gctgcccggc aggccggctg
     1501 ggtgtgggtc tggtcacagg tcgccggagg gcgctccgga gccggggagc cgtgcgcgag
     1561 cgcccggtgg atcgtggggc gaccgtgcga tttttccgcc cgtgtggcaa cagcgttcgc
     1621 tcaagccaca atgcggcagg gcctcgggtt gacgccggcg cacagctctg cacgctgggc
     1681 atgtgggggc gcagctcgac ttccggggac tggtcgatgc agcgacggac gtgatcgccg
     1741 tgttcgatcg cgaccggcgc tacgtctaca tcaatgccgc cctggagcgc atcaccgggg
     1801 tgccgggggg cagcgtgctc ggcaagcgca acgacgaggt gatgccggcc gacgacgcgc
     1861 tggtgtggac cgaggcgctc gacgacgtca gccgctcggg ccggccgcgc gcgatcgagt
     1921 ccacgctcgc gacgccgcac ggcccgcgcc ggttcgcctc ggtgctcacc acggtgtccg
     1981 acggcctggt ctgcgcggtg tcgcgcgaca tcaccgagat cggccggcgc tacgacgagg
     2041 tcgacctcga catgttcacc gagctcgcgc ggcgcaccgg cgtggcgctc gacaacgcgc
     2101 ggctgctggc ggcggagcag gaggcgcggc gccacgccga ggaggcgcgc gaccgcacgc
     2161 gccggctcca gcagctgacc gcggcgctgt cgggcgcgct cgacgagcac gacgtggtct
     2221 cgatcatggt caacgcgggc cgcgacgcgg tcggcgccgg cgccgggttc gcctggctgc
     2281 tgcgcggcgg cgatacgctg gagctggccg gctccgcgca cgacgacagg ccaatccggc
     2341 tcgacagctt ccaggccatc gcgatgaccg cgccgctgcc ggtgtacgat gtcgtacgca
     2401 gcggtcagcc gatgctgttc gagagcctcg ccgcgctgaa ggcgagctat ccccaggcgg
     2461 ccccggccag cgacctgccg ttccgctcct gggcggtgat cccgatcgtg ctcgccggcc
     2521 gcggcgcggg cgcggtgtcg ttctcgttcg ccgaggagcg cacgtttcgc gacgacgatc
     2581 gcgagctgct caccgcgatg gcgggtcagg cctcgctcgc gctcgagcgc gcccggctgt
     2641 acgacgcgct gcagaatacc gcgcagcagc tggcgaccgc caaccgccgc aaggacgagt
     2701 tcctcgccat gctcggccac gagctgcgca acccgctcgc gccgatcgcg accgcgctcg
     2761 acgtgatgga gatcaaggac cccagcgcga tgcgcctgga gcgcgaggtg atccgtcgcc
     2821 aggtcgatca cctgtcgcgg ctgatcggag atctgctcga cgtctcgcgg atcacgcgcg
     2881 gcaagatcca gctgacccgc gaggtcctgg agctcggcac cgtgctcgcc gccgcgatgg
     2941 agatggcggc cccgatgctg gagcggcgca tgcagcagct cgcggtcgac gtcccgtacg
     3001 gaggcctgct ggtcgacgcc gatccgacgc ggctcgccca ggtgttccag aacctcttga
     3061 tcaacgcggc caagtacagc gagccgcggt cggagatcac gttgcgcgcg cgcgggcgta
     3121 gcgaccatat cgtcgtcgag gtcgtcgatc agggcatggg gatctcgccc gacctgctgc
     3181 cacggctgtt cgacctgttc gtccagggcg agcgcgcgct cgatcgctcg cagggcggcc
     3241 tcgggatcgg gctgacgatc gcgcgcagcc tgtgcgagct gcacggtggc acgatcacgg
     3301 cggccagcgc cggccccggc cgcggcagca cgttcaccgt gacgctgccg cgggcggag
//
LOCUS       DASVOR010000752         1862 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_386998,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000752 DASVOR010000000
VERSION     DASVOR010000752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1862
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_386998"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..829
                     /locus_tag="VF516_14875"
     CDS             <1..829
                     /locus_tag="VF516_14875"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830301.1"
                     /note="cleaves the ring of
                     2,3-dihydro-2,3-dihydroxybenzoyl-CoA forming
                     6-hydroxy-3-hexenoyl-CoA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA-dihydrodiol lyase"
                     /protein_id="HEX8109013.1"
                     /translation="VVQGPDAPPPAELGDPRGGPALADTWSLRAFRELDDALLRIRFD
                     LADIGVITVRTVGDPKLVIAHDEALTKSTTGFAREVRLLQRRVLKRFDNTARSFYAVA
                     DRPDACFAGSLLELALGADRFYMLIDRDEKIAVATSVANQGFLTMSSGLSRLDARFYG
                     EPGRAQKVLARGSEGPIPSEEAEELGIATIAADDIDFEDELRIAIEERASLSPDALTG
                     MEQSLRFVGPETLETKIFGRLSAWQNWIFTRANSTGEHGALTLYGQPERPQFQWKRT"
     gene            832..>1862
                     /gene="boxB"
                     /locus_tag="VF516_14880"
     CDS             832..>1862
                     /gene="boxB"
                     /locus_tag="VF516_14880"
                     /EC_number="1.14.13.208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0010128 - benzoate catabolic process via
                     CoA ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX8109014.1"
                     /translation="MTAVLTDEKIPNNVNLSSDKRLQRALEAWQPHFIDWWKQMGPVG
                     WQERDVYLRTAVSVETDGWAHFDYVKMPEYRWGIFLEPKQDGRTHGFGDFHGQPVWDE
                     VPGEFRNMLRRLVVTQGDTEPASVEQQRHLGATAPSLYDLRNLFQVNVEEGRHLWAMV
                     YLLHTYFGRDGREEAEELLARRSGDADKPRILGAFNEPTSHWLSFFMFTMFTDRDGKY
                     QLSALAESGFDPLARTCRFMLTEEAHHMFVGETGVQRVVKRACELMKQDPSGDPRALG
                     GIDLPTVQKYLNLWYSLSLDLFGGEVSSNAADAFAAGLKGRYKEDRYDEHKLVGSHFA
                     MDVLEGGKVV"
BASE COUNT          291 a          675 c          629 g          267 t
ORIGIN      
        1 cgtcgtccag ggccccgacg cgccgccgcc cgccgagctc ggcgatccgc gcggcggccc
       61 ggcgctcgcc gacacgtgga gcctgcgcgc gttccgcgag ctcgacgacg cgctgctccg
      121 gatccggttc gacctggccg acatcggcgt gatcaccgta cgcacggtcg gcgatccgaa
      181 gctggtgatc gcgcacgacg aggcgctcac gaagtcgacc accgggttcg cccgcgaggt
      241 tcgcctgctc cagcgccgcg tgctcaagcg gttcgacaac accgcgcgca gcttctacgc
      301 ggtcgccgac cgccccgacg cgtgcttcgc cggctcgctg ctcgagctcg cgctcggcgc
      361 cgaccggttc tacatgctga tcgacaggga cgagaagatc gcggttgcca cctcggtcgc
      421 caaccagggc ttcttgacca tgtcgtcggg gctgtcccgg ctcgacgcgc ggttctacgg
      481 cgagcccggc cgcgcccaga aggtcctggc gcgcggcagc gaggggccga tcccgtcgga
      541 ggaggccgag gagctcggca tcgcgacgat cgccgccgac gacatcgact tcgaggacga
      601 gctccgcatc gcgatcgagg agcgcgcgtc gctgtcgccc gacgcgctga ccggcatgga
      661 gcagtcgctc cggttcgtcg gccccgagac cctcgagacc aagatcttcg gccgcctgtc
      721 ggcctggcag aactggatct tcacccgcgc caactcgacg ggcgagcacg gcgcgctcac
      781 cctgtacggc cagcccgagc ggccgcagtt ccagtggaag aggacttgat catgaccgcc
      841 gtgctcaccg acgagaagat ccccaacaac gtcaacctgt cgagcgacaa gcgcctgcag
      901 cgcgcgctcg aggcctggca gccgcacttc atcgactggt ggaagcagat gggcccggtc
      961 ggctggcagg agcgcgacgt ctacctgcgc accgcggtgt cggtcgagac cgacggctgg
     1021 gcgcacttcg actacgtcaa gatgcccgag tatcgctggg gcatcttcct cgagcccaag
     1081 caggacggcc ggacccacgg gttcggcgac ttccacggcc agccggtgtg ggacgaggtc
     1141 cccggcgagt tccgcaacat gctgcgccgg ctggtcgtca cccagggcga caccgagccg
     1201 gccagcgtcg agcagcagcg ccacctcggc gcgaccgcgc cgtcgctcta cgacctgcgc
     1261 aacctgttcc aggtcaacgt cgaggagggc cgccacctgt gggcgatggt ctacctgctc
     1321 cacacctact tcggccgcga cggccgcgag gaggccgagg agctgctcgc ccggcgctcg
     1381 ggcgacgccg acaagccgcg catcctcggc gcgttcaacg agccgacctc gcactggctg
     1441 tcgttcttca tgttcacgat gttcaccgac cgcgacggca agtaccagct gagcgcgctg
     1501 gccgagagcg gcttcgaccc gctggcgcgg acctgccggt tcatgctgac cgaggaggcc
     1561 caccacatgt tcgtcggcga gaccggcgtg cagcgggtgg tcaagcgcgc ctgcgagctg
     1621 atgaagcagg atcccagcgg cgatccgcgc gcgctcggcg gcatcgacct gccgaccgtc
     1681 cagaagtacc tcaacctgtg gtactcgctg tcgctcgacc tgttcggcgg cgaggtgtcg
     1741 tccaacgccg ccgacgcgtt cgccgccggc ctcaagggcc gctacaagga ggaccgctac
     1801 gacgagcaca agctggtcgg ctcgcacttc gcgatggacg tgctcgaggg cggcaaggtc
     1861 gt
//
LOCUS       DASVOR010000753         7908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_415867,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000753 DASVOR010000000
VERSION     DASVOR010000753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7908
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415867"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF516_14885"
     CDS             complement(<1..321)
                     /locus_tag="VF516_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109015.1"
                     /translation="MTRWGASRTYQQLAATWINGLKLHAAILPLAEFAAVIGNLRGAR
                     FGRDGAWRASCLFGALDEARHTQIPLLLMHELLRWDPQFDWTHKLHHTNNWVAIAARH
                     LTDEL"
     gene            complement(638..1435)
                     /locus_tag="VF516_14890"
     CDS             complement(638..1435)
                     /locus_tag="VF516_14890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109016.1"
                     /translation="MSRSASGGRWDPVFGWVSAAQRREADLFYADLVGRPARRPLARA
                     RFGEAADDASEAIDVNDSRRKTFREMWDLIVKFRWQGTPAVFTNDLILCICWEESTFL
                     NIWQQNDSGALGPAKGFGQLEPSALALIRRKFAFTESVAELQRLMDQDAFSVQFIGRV
                     LHTLFDNLRAQYPSLRSDDEATKRSALLNGYAGYLTDHAQWRKDIVDGWYAAERLLVA
                     GHGPSGYPTRTAAQQALDAATPANRKMRDRAAWTAFLAQLCQTLPDP"
     gene            complement(1565..2002)
                     /locus_tag="VF516_14895"
     CDS             complement(1565..2002)
                     /locus_tag="VF516_14895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109017.1"
                     /translation="MSAAQHSLDQARSLARIAALEADPTVYVEQLAAELPGAATLVEL
                     EARDAALVAALGQIDAMIARAMRIRLDHALANDTSIGAPTRSVFASTITGYAGRLSLL
                     GARSTSSSRSRTRTRCGSGYAMAFRSRTGRSRRGCAGQTTSTS"
     gene            complement(2166..2336)
                     /locus_tag="VF516_14900"
     CDS             complement(2166..2336)
                     /locus_tag="VF516_14900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109018.1"
                     /translation="MKKTTRDKKFGLARETIRELTSPEDKAALARVVGGSCAPMAPST
                     HCTALCIDDAEA"
     gene            complement(2416..3666)
                     /locus_tag="VF516_14905"
     CDS             complement(2416..3666)
                     /locus_tag="VF516_14905"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEX8109019.1"
                     /translation="MSLAWRPLFSGDLAAQMLGIVDDIADATTGPTEHPGNLPAEDAP
                     AWRCSLDALAGQSLLHAYLALHGGTEARADTAIALLDQATDAAGVPAVRPGLYYGFAG
                     TSWVASHLAGRLFEETQDNCLEVDEVLLATLADPMWNARYELLSGVVGMGVYARERLP
                     RPSAVRLIEAVIGRLESCAERNAAGVAFFNPVETLSPEYRDVASRGWYNLGIAHGIAG
                     VISFLGSLCRLGVAVERAAPLLADSVAWLLTRELPAGGDYRFKCTYTPDRDIDICRLG
                     WCHGDLAVATALLVAARGAGEPSWEQHARRIARSAAALAVDSTPVPDASLCHGAAGIG
                     HLFNRLYQETDEPSLGEAARSWLRKAVAMREPGHGFGGYQLYERGKKYNNPGFRVGSS
                     GIALALLAAASPVPPDWDRLLMAS"
     gene            4058..4924
                     /locus_tag="VF516_14910"
     CDS             4058..4924
                     /locus_tag="VF516_14910"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8109020.1"
                     /translation="MNYAAISPTARLVAYFRRRSDVPLAGTIAERIGAADAVRAMLGG
                     ADVAPGVVKWMAPLFEARYRSVEREIRAAGVRQVVELAAGFTFRGAALAGPALRYVET
                     DLPALHAQRAALRAALEHDGAIAAAPELVFAAADASSVDAIVGLGDLLIPGAPVAVVH
                     EGLLQYFSMAEKREVARGVAALLARFGGVWITPDLERLGDPLFARWTDPQFRAIAGFL
                     SDSTRRHMPDAAFPSHDAIPTFFAELGFAAAARPQLDGSFALRSAGPAGAGEDDLAAL
                     QASRQIWTLRLR"
     gene            5111..6076
                     /locus_tag="VF516_14915"
     CDS             5111..6076
                     /locus_tag="VF516_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109021.1"
                     /translation="MSNPRYGIRATALALATASAGCGGSGTSQPPLPLSESNATSVAA
                     EALIFTELPADSQIGGIMPGVAAGLRSLDPGPVQWLTALATGPQNVDGTVTNPCDVSG
                     TVTVTTAGTTTTITFTNCMDNTSSTLNGTLRSTRQTSSTSNQISTTVSFNLTGTQGSQ
                     TFAESGGYTELRTLGQTSSDPTESELTGDSFSMSFSVDGKLRDQVTMSSFDIDTLEQL
                     TPTEQHVEHFTYEVDSSRLRGHIGVMTTQDLKTGLNVFPFTGQILISGANHTRLQITV
                     LGDEIFMPPAGQGQLELQIDPGTGTFGAPIWANWLTLSALATIER"
     gene            complement(6127..7506)
                     /locus_tag="VF516_14920"
     CDS             complement(6127..7506)
                     /locus_tag="VF516_14920"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX8109022.1"
                     /translation="MVLTARRGLGIALSAALAAAACNHSLFDNRPGGGSAGSGSDAGS
                     DGPGVPASCPAGCLGDAAADFDGSATGMTHVWRYLEDQGRSWQAMTLSNSTMTGQIDP
                     ANRITTCENQKGAPACAALPDALLMSSGTSAVDPAIEFTTPTDRPAQVIQLILHAYLA
                     SGTGQTIRIYRNSREDLLFTSPLTATSRVDHAVVVDALPGDRFLVAISGDAAASEVGL
                     QLYVNATGLHFPNSCQLALSFAGVNTTTTTTVQDPCSKYTFTSLEFTAPPPAQTFAAT
                     PFVPGPGPYPEQMPGVSVGQGMFLQRDGAAPMNALDWSHDTTVQLWAQPTMLDQLKSD
                     SFLFDDFDIEACGGVELSVGEVSGTDPHITLHGCTDPVTMADSAITPAPFFPPDSAWH
                     FVRVVRNAAGFDLCIDGVYVASLPVKPGPIASNNPPDLGKPLVYNEGASYVGGVDDVR
                     VFTAALPCR"
     gene            complement(7497..>7908)
                     /locus_tag="VF516_14925"
     CDS             complement(7497..>7908)
                     /locus_tag="VF516_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109023.1"
                     /translation="DAGLAPPLGVRFAIGAGFGTNLRLAGETGAPSLGAAVAIGARFE
                     LGLDAVMVAYAIIPSIRIRIAGDGLALHAIGAVPYSLTSGSMSERFAAPAIGLGLRYR
                     PTPGLAVRLEGYAAFAGRDHGTAIPVFLGAERWF"
BASE COUNT         1144 a         2808 c         2813 g         1143 t
ORIGIN      
        1 cagctcgtcg gtgaggtggc gcgcagcgat cgcgacccag ttgttggtgt ggtgaagctt
       61 gtgggtccag tcgaactgcg gatcccagcg caacagctcg tgcatgagca aaagcgggat
      121 ctgggtgtgg cgcgcctcgt cgagcgcgcc gaacaggcag ctcgcccgcc acgcgccgtc
      181 ccgaccgaac cgcgcgcccc gaaggttccc gatcacggcg gcgaactcgg cgagcggcag
      241 gatggcggcg tgcagcttga gcccgttgat ccaggtcgct gcgagctgct ggtaggtcct
      301 cgatgcgccc catcgcgtca cgcaccgagt cgaccgccgc gtccttgtcg gcctgcgtcg
      361 tgacgtagtc gctgaacacc ggccggtacg gctcgtccca gtcccgccac gccgcgtgcg
      421 gcagccacgg ctcaccgctc gcgactgcgg gaaatacctg ctcgaccggg acgtacgagt
      481 agtcccaatc gagtttcctg cggaggccca gccatgtgct tcgcggcagc atcgaccttg
      541 gggcgggcta gcgcgatcgt gcatcgtgtc cacaggcggc ttcatcgggc ctgggaccgt
      601 ggcacgagag gcgcgcaccg tcgcgggtgc gaccgcgcta cggatcgggc agcgtctggc
      661 agagctgcgc gaggaacgcg gtccaggcgg cgcggtcgcg catcttgcgg ttcgccggcg
      721 tggcggcgtc gagcgcctgc tgggccgccg tgcgcgtcgg gtagccgctc gggccgtgac
      781 cggcgaccag caggcgctcg gcggcatacc agccgtcgac gatgtccttg cgccactgcg
      841 cgtggtcggt caggtacccg gcgtagccgt tcaggagcgc gctccgcttc gtcgcctcgt
      901 cgtcgctgcg caggctcggg tactgcgcgc gcaggttgtc gaacagcgtg tgcaggacgc
      961 ggccgatgaa ctgcacgctg aacgcgtcct ggtccatcag gcgctgcagc tcggccaccg
     1021 actccgtgaa cgcgaacttg cggcgaatga gggccagcgc gctcggctcg agctggccga
     1081 accccttcgc gggaccgagc gcgccgctgt cgttctgctg ccagatgttc aggaacgtgc
     1141 tctcttccca gcagatgcag aggatgaggt cgttcgtgaa cacggccggc gtgccctgcc
     1201 agcggaactt cacgatcagg tcccacatct cgcggaacgt cttgcggcgg ctgtcgttga
     1261 cgtcgatcgc ctcgctcgcg tcgtcggcgg cctcgccgaa gcgcgcgcgc gccagcggcc
     1321 ggcgcgcagg gcggccgacg agatcggcgt agaacagatc ggcctcgcgg cgctgcgccg
     1381 ccgacaccca cccgaacacc ggatcccacc tgccaccgct cgcggatcgg ctcatcacgg
     1441 cctcctggtc gtcacgcctc gtggcgcgcg gcgggatagt aacacgcgcc gcaagatgcg
     1501 ccgccgtgat ggcaaggtgg tcagtggtcc agctgcccag cgatcgctgg ctccagctcc
     1561 cgggtcagct tgtcgacgta gtttgacccg cgcagcccct cctggatcgc cctgtcctgg
     1621 atcgaaaagc catggcgtag ccagaaccgc agcgcgttcg ggttctgctc ctggacgatg
     1681 aggtggagcg cgcgcccagc agcgacagcc gcccggcgta gcccgtgatc gtcgacgcga
     1741 acacgctccg ggtcggcgcg ccgatcgagg tgtcgttcgc gagggcgtga tcgaggcgga
     1801 tccgcatcgc gcgcgcgatc atcgcgtcga tctggccgag cgcggcgacc agcgccgcgt
     1861 cgcgcgcctc gagctcgacg agcgtggccg caccggggag ctcggcggcg agctgctcga
     1921 cgtacacggt ggggtcggcc tcgagcgccg cgattcgcgc gagcgagcgg gcctgatcca
     1981 ggctgtgttg cgccgcactc atggcacggt ctttcgcaca gcagtgatta tgccacgcgg
     2041 cgcgattcgc gggccggccc atcacccgcc ggcggcggta ccgccgaagc aagccgctca
     2101 cgccgcgacc gccgggaccg cagacatgcg tttgcgattc cggcctggtt cgctcggcgc
     2161 ggcgatcagg cctccgcgtc gtcgatgcac agggccgtgc agtgcgtcga cggcgccatg
     2221 ggggcgcagc tgccgccgac gacccgggcc agcgcggcct tgtcctcggg actggtcagc
     2281 tcgcggatcg tctctctggc caatccaaat ttcttgtctc ttgtcgtctt cttcattgcg
     2341 gactcctttt gtgtcgtttg cagtcaccgc attgcgacag cagcgcggtc gagcgatgtc
     2401 ggcccggacg ggctatcacg aggccatcag caggcgatcc cagtccggtg ggaccggcga
     2461 cgccgcggcg agcagcgcca gggcaatgcc gctcgatccg acgcggaacc ctgggttgtt
     2521 gtacttcttg ccgcgctcgt acaactggta gccgccgaac ccgtgcccgg gctcgcgcat
     2581 cgcgacggcc ttgcgcagcc agctccgcgc ggcctcgccg agcgatggct cgtcggtctc
     2641 ctgatagagg cggttgaaca ggtggccgat accggcggcg ccgtggcaca ggctggcgtc
     2701 ggggaccggc gtgctgtcga cggcgagcgc cgccgcagac cgcgcgatcc ggcgcgcgtg
     2761 ctgctcccag ctcggctcgc cggcgccgcg ggccgcgacc agcagcgcgg tggcgacggc
     2821 gaggtcgccg tggcaccagc cgaggcggca gatatcgatg tctcgatccg gtgtataggt
     2881 gcacttgaac cggtagtcac cgcccgccgg gagctcgcgc gtgagcagcc acgcgacgct
     2941 gtcggcgagc agcggcgcgg cgcgctcgac ggcgacgccg aggcggcaca gcgagccgag
     3001 aaacgagatg acgccggcga tgccgtgcgc gatgccgagg ttgtaccagc cgcgcgacgc
     3061 cacgtcgcgg tactccgggc tgagcgtctc gaccgggttg aagaacgcca cgccggccgc
     3121 gttgcgctcg gcgcacgact cgagccggcc gatcacggcc tcgatcaggc gcaccgccga
     3181 gggccgcggc aagcgctcgc gggcatagac acccatcccc acgacgccgc tgagcagctc
     3241 gtacctggca ttccacatcg gatcggcgag cgttgcgagc agcacctcat cgacctcgag
     3301 gcagttgtcc tgggtctcct cgaacagccg gccggcgagg tgggacgcga cccagctggt
     3361 ccccgcgaag ccgtagtaca gcccgggccg cacggcaggc acaccggcgg cgtcggtcgc
     3421 ctgatcgagc agcgcgatcg ccgtgtcggc gcgcgcctcg gtcccgccgt gcaaggcgag
     3481 gtaggcgtgc agcaggctct gaccggccag ggcgtcgagc gagcaccgcc aggccggtgc
     3541 gtcctcggcg ggaaggttgc cggggtgctc ggtcggaccc gtggtcgcgt ccgcgatgtc
     3601 gtcgacgatg ccgagcatct gggccgcgag atcaccggag aacaggggac gccacgcgag
     3661 actcatgcgc tcgagtctac gcagcgcgcg gcgcgcgatg tggctgcccg gcgcgatcct
     3721 tgctgtcatc gcttgcccgg gaagcgaccc tggcggactg catgacctgg tcgagtgccg
     3781 gtcgagccag caagtcgccg tcgatcattg ccatggatgc gctgcatacg gagcatctgg
     3841 cgcatcatgc tgcgggacat cggctgcaag ttcccgaagt gcatcgcaat gcggctccct
     3901 ccgcgcaacc gcagctgtgc tcgcccgcac ggcgccgacg caatggcagc ctgaacctgc
     3961 gcgacgcgca cgcatcaccc gcctgccggg cagcctcgtg ccgggatcgg gcgtcggcgc
     4021 ggcccaccgc ttgcccgccg cggcgcatcc taccgggatg aactatgcag ccatcagccc
     4081 gaccgccagg ctcgtcgcct actttcgccg gcgatcggac gtgccgctcg ccggcacgat
     4141 cgccgaacgg atcggcgccg ccgacgcggt gcgcgcgatg ctcgggggcg ccgacgtcgc
     4201 gcccggcgtg gtgaagtgga tggcgccgct gttcgaggcg cgctaccgca gcgtcgagcg
     4261 cgagattcgc gccgccggcg tgcgccaggt cgtcgagctg gcggccggct tcacgttccg
     4321 cggcgccgcg ctggccggac cggcgctgcg ctatgtcgag accgacctgc cggcgctgca
     4381 cgcccagcgc gccgcgctgc gcgccgcgct ggagcacgac ggcgcgatcg cggcggcccc
     4441 ggagctggtg ttcgctgccg ccgacgcatc gtcggtcgac gcgatcgtcg ggctcggcga
     4501 cctgctgatc cccggcgcgc cggtcgccgt ggtccacgag gggctgctgc agtacttctc
     4561 gatggccgag aagcgcgagg tcgcgcgcgg cgtggcggcg ctgctcgccc ggttcggcgg
     4621 cgtgtggatc acgcccgacc tcgagcgcct cggcgatccg ctgttcgcgc gatggaccga
     4681 tccgcagttc cgcgcgatcg cgggcttcct gagcgactcc acgcgccgcc acatgcccga
     4741 cgccgcgttc cccagccacg acgcgatccc gacgttcttc gccgagctcg ggttcgccgc
     4801 cgccgctcgc ccgcagctcg acggcagctt cgcgctgcgc tccgcgggcc cggcgggcgc
     4861 cggcgaagac gacctggccg cgctgcaggc cagccggcag atctggacgc ttcgcctgcg
     4921 ctaggccggc gaccgacatc cggacggctg cgggagctgc gtgaggtgat cacggcgccg
     4981 tccttgcgct ttgccgggag ggccaccccg caacggcgag ccaggccatc atgacattcg
     5041 cgtgatcccg cgagacgcat ccaccgcagc gatgagatcc tccccgccgg aaccatcgag
     5101 gaccacgcac atgtcgaatc cacgctacgg aatccgggcc acggccctgg cgctggcgac
     5161 cgcctccgcg ggctgcggcg gctccggaac ctctcagccg ccgctgccgc tcagcgagag
     5221 caacgcgacg tcggtcgcgg ccgaggcgct gatcttcacc gagctgcccg ctgactcgca
     5281 gatcggcggc atcatgcctg gtgtggcggc ggggctgcgc agcctcgatc ccggcccggt
     5341 gcagtggctg accgcgctcg cgaccggccc gcagaacgtc gacggcacgg tcaccaaccc
     5401 gtgcgatgtc agtgggacgg tgacggtcac caccgccggc acgacgacca cgatcacgtt
     5461 caccaactgc atggacaaca ccagcagcac gctcaacggc accctccggt ccacccgcca
     5521 gacgtcgagc acctcgaacc agatctcgac gaccgtgtcg ttcaacctca ccggcacgca
     5581 gggctcgcag acattcgcag agtccggggg ctacaccgaa ctgcgcacgc tcggccagac
     5641 ctcgtcggac cccaccgagt ccgagctgac cggagacagc ttcagcatgt cgttcagcgt
     5701 cgacggcaag ctgcgcgatc aggtcacgat gtccagcttc gacatcgaca ccctcgagca
     5761 gctcaccccg acggagcagc acgtcgagca cttcacctat gaggtcgaca gctcccggct
     5821 ccggggccac atcggcgtca tgaccaccca ggacctcaag accggcttga acgtcttccc
     5881 gttcaccggc cagatcctga tcagcggcgc taaccacacg cgcctgcaga tcacggtgct
     5941 cggcgacgag atcttcatgc cgcccgccgg tcagggccag ctcgagctcc agatcgatcc
     6001 gggcaccggc acgttcggcg cgccgatctg ggccaactgg ctgacgctgt ctgccctcgc
     6061 cacgatcgag cggtagaccc gcgtcgccgg tgtgcgtcct gtatcgaccg aggtcgtcgg
     6121 cccggatcag cggcagggca gcgcggcggt gaacacgcgg acgtcgtcga ccccaccgac
     6181 gtacgacgca ccctcgttgt acacgagcgg cttgcccagg tcgggcgggt tgttcgacgc
     6241 gatcgggccg ggcttgaccg gcaggctcgc gacgtagacg ccatcgatgc acaggtcgaa
     6301 cccggcggcg ttgcggacca cgcgaacgaa gtgccaggcg ctgtccggcg ggaagaaggg
     6361 cgcgggcgtg atcgcgctgt ccgccatcgt caccgggtcg gtgcagccgt gcagcgtgat
     6421 gtgcggatcg gtcccgctga cctccccgac cgagagctcc accccgccgc acgcctcgat
     6481 gtcgaagtcg tcgaacagga acgagtcgct cttcagctgg tcgagcatgg tcggttgcgc
     6541 ccacagctgc accgtggtgt cgtgcgacca gtcgagcgcg ttcatcggcg ccgcgccgtc
     6601 gcgctggagg aacatgccct ggccgacgga gacgccgggc atctgctccg ggtaggggcc
     6661 ggggcccggc acgaacggcg tcgccgcgaa ggtctgcgcg gggggcggag cggtgaactc
     6721 gaggctggtg aacgtgtact tgctgcacgg gtcctgcacc gtggtggtgg tcgtggtgtt
     6781 caccccggcg aacgacagcg cgagctgaca gctgttcggg aagtgcagtc cggtggcgtt
     6841 gacgtagagc tgcaggccga cctcgctggc ggccgcatcg ccgctgatcg cgaccaggaa
     6901 gcggtcgccg gggagcgcat cgaccacgac ggcgtgatcg acgcggctgg tcgcggtgag
     6961 cgggctggtg aacagcaggt cctcgcggct gttgcgatag atccggatcg tctggccggt
     7021 gccgctcgcc aggtaggcgt gcaagatgag ctggatcacc tgggccggcc tgtccgtggg
     7081 ggtggtgaac tcgatcgcgg ggtcgaccgc gctggtgccc gacgacatca gcagggcgtc
     7141 gggcaacgcg gcgcacgccg gggcgccctt ctggttctcg caggtcgtga tccggttggc
     7201 ggggtcgatc tggcccgtca tcgtcgagtt gctgagggtc atcgcctgcc aggacctgcc
     7261 ctgatcctcg aggtagcgcc agacgtgggt catgccggtg gcgctgccgt cgaagtccgc
     7321 ggcggcgtcg ccgaggcagc ccgcggggca cgacgccggg acgcccgggc cgtcggaccc
     7381 ggcgtcgctg ccactcccgg cgctgccgcc gccggggcgg ttgtcaaaca gcgagtgatt
     7441 gcaggccgcg gccgcgagcg cggcggaaag cgcgatcccg aggccacggc gagcggtcag
     7501 aaccatcgct ccgctccgag gaacaccggg atcgccgtgc cgtgatcgcg accggcgaac
     7561 gcggcgtagc cctcgagccg gacagccagg ccgggggtcg gccggtagcg cagccccagg
     7621 ccgatcgccg gggcggcgaa ccgctcggac atcgacccgc tggtgagcga gtacggcacc
     7681 gcgccgatcg cgtgcagcgc caggccgtcg ccggcgatcc ggatccggat cgacgggatg
     7741 atcgcgtacg ccaccatcac ggcgtcgagg ccgagctcga accgcgcgcc gatcgcgacc
     7801 gcggccccga gcgatggcgc gccggtctca ccggccaggc gcaggttggt gccgaacccg
     7861 gcgccgatcg cgaaccgcac cccgagcggc ggcgcgaggc cggcgtcg
//
LOCUS       DASVOR010000754        15926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_240945,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000754 DASVOR010000000
VERSION     DASVOR010000754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15926
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240945"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /locus_tag="VF516_14930"
     CDS             <1..2197
                     /locus_tag="VF516_14930"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8109024.1"
                     /translation="DSTAGAASTPAPVEGKPEARPFPKQPGPAKRVEAMPRGPAPLRI
                     DGKLDEPAWATAKPAGDFFRRWPTEGWPSQPTTFRVLYDDQALYFGIVSILAPGEKPR
                     ITNLKRDAVTRFWYDDNVQLEIDVAHGKQDLMLFGVNAAGVQLDAMQLDNGRQFVIEW
                     DAQWESASHVEGNVWTVEIKIPASELGGLSRLATIGINVVRNDMMLAETHSWSLIPQG
                     LPLEHTPLYGELHGTDVGTHTPLVAEAYVLGAAPGLADTLNTHDRDYGAKAGLDLRTR
                     LGASTFVEASVLTDFAQVELDNQQLNLDRFALFYPEKRDFFLSGLDVFNFGRAGIAQP
                     LFTRTIGLDANGDIERVWGGLKAYRRNDRFSFGLLDVVTAETANSPLTNWAAGRARVR
                     VGDATSIGVLGTLKQPLTQGTSAPAATENFTAGADFESRFTDRATASGFGSVSATPDT
                     TNPHGVLAGGDLSVGGDVFGPKLSLLYVSRHYDSQTGFVARTDIASIDGLLPVRYFFA
                     NERIRALELDVTGGVTTDAAIRHLETGYVSGYVTAETTGSIKPYVSWRALHDVVSDDF
                     ALSPALTVHAGTYRSVTGVMGVSTTFPAMVLLDLSYSPTTGFFGGERQSISESLSAPL
                     GQHVLVVENATGAWVKLGDQHDFLYVVNGELDLRASRQLGLEMLATYDSVKAAFQGQA
                     RLRWDPIAGTSVFLVYRDDRRTAMTAGTRSTTADDHSLVLKVTSLFNVL"
     gene            2204..4171
                     /locus_tag="VF516_14935"
     CDS             2204..4171
                     /locus_tag="VF516_14935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109025.1"
                     /translation="MHMVRIHPVLALVAAVLAACQGHATTPPAAPPAPRAVPAAVADP
                     PASPDHLELIRALMHAPEIAFDGAAFPEPRERIRAYVRALLADDATYPGLLSALYAFT
                     RYPMYPSAVPFLVQHTVIDGTPVFYGHYAHDKGATGPASAPPPCPKSAAVKVVPWWDP
                     STEVLVCPDSYRPNVRMFAKDRDCGTLSDFQLLVATRSPQENICGCGPNLIYCAPNEE
                     IDWKIRGAAQAEFIDTIAYFIKSELPFQDILTTPATMRSRIGDFFYLKSRLLAGEHVD
                     WNELQGDDKAMRPRPWGLGGGVLSTPQVRWFDCADRVVIAHIWEDFLCIAQESNRVDT
                     EILLHGTKTANLRRNLDHLAEVPGCRDCHARMEFGSRFLEFSTGKDDGTTYSPERSPR
                     ESRIYGTSADDVLWEGHATSTNLGRVLSQQPQFVDCQVDKVLKRFYGNDPVPSGVRDE
                     LRDRFDRSRNFAQLLSDTAVARFAPSAPPPAARAWTEIAEDACDACHDGSDARRKRMP
                     PADRRDAIQAARRVANYAMPPSPGRMDDAERRTLAMGLCQSAGLRAGECTDMLYPFDA
                     SADLLPLDTLIERITAQRSGDPSPEFTAILNELKSNFRGELAAAAPDAPPEDSLNVSA
                     YLYLLTNDACAARPDRATCIAKLLDADRRRR"
     gene            4182..5396
                     /locus_tag="VF516_14940"
     CDS             4182..5396
                     /locus_tag="VF516_14940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109026.1"
                     /translation="MLDRRQFLALGATTAVAGALHRTALAQPAAQASLPHAPVRPRTF
                     VQIILRGGFDPILATDPKDPEQVARDIDVPYRDREVVERRGRRFGPVLRPLGALLDDL
                     VVVRGIKIYTVSHPAAMRRLLELRNEVARNDRLTPGFTGVLDGQLPWRPIGCLRQLGG
                     IEAGIGAARDLSVRYEQIGTLERLRRLAKTGERCPQDPRLDTLLQHLAAANEPVKVTT
                     PRLPAVIGRNSAEDTTQTWSRYLADLFFVLENDLANTVLLYAPFPYWDSHQANRAIQH
                     GCWAVFAPILATILGELRERKSPRGCPLADEVGLLVSSEIGRFPRLNSYAAGKDHFPE
                     ISCLLSGPGLNRTAIGATNERMAGTMIADGGTRRFLELDDLGASILQWFGVTDRERLG
                     YLGRGVPGLLAG"
     gene            5459..7075
                     /locus_tag="VF516_14945"
     CDS             5459..7075
                     /locus_tag="VF516_14945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109027.1"
                     /translation="MIAPDSVTAPAACAPSARAGWLAALTIAAAGMLVALYHADSRAL
                     HHDEAASLRKSQYSWDQLVHPRQFEDAPTLPNQAGPSANTTSELLNRWLFIAGFKVWT
                     DAFGSSPLAMRGFSIGFHGLFVLAFYALLLELLRESSTRTVPRPRIVALIALALGTLN
                     ALALHQAGQVRNYSLCTTLLMLTAWAILRAARRGRWTDWALVAVFGIAAFYSFNVAMM
                     TLAGLWTWFGLRRLFRAPARAESPVARTSHAPWIAAAGTTVLALLPAVLHLASQRGSH
                     GAAGWWMPVLTWSRFFEFAPGFVFGDSLFLHNSPATGVAILAAFALALYRTWRIGFLL
                     PVVAGLLPFLALVAASLTGPNVVIPRYFLVLSPFLYTCMAIALCTIPSHRLQLAAVAG
                     VIAVVLAFSVRSVTTWDRQSAGGSREIAAWIQRNVEPGATVIVSPWLYLAVRYYFPPE
                     ADVRVYVRADTGIPFYMSGPLLDRRDRVTSLSDLIGDPPKARHAAYVHVGINEPPIDP
                     VALPEGWTVEQRKRFESPFVISEVVVVRTH"
     gene            7170..8453
                     /locus_tag="VF516_14950"
     CDS             7170..8453
                     /locus_tag="VF516_14950"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8109028.1"
                     /translation="MSANRPVGLLEVVVNNRNFRLLWLAQLISQLGDWFNVVATATLV
                     GYLTGSAAAIGVLFAVRLAAPVFVSPLAGVCADLMNRKHILIASDIARALTMLGFLLI
                     RTPDDIWLVYTLTAIQLGLSGLFYPTREAILPDLARGGELVAANVLGMASWSVMVSVG
                     TAIGGVIAGVWGPAPAFMIDAMTFVISALLLAQIRYAPVLRAEHARPSVRSAIAQYGH
                     SLAYLRANPAIFIAVLHNVFIAAFAATTSDIFAAGIARDVFVYGAGGSISLGIMLACS
                     GLASGMAPGSFLRTFGGTFARKHHAIIAGYAIIAIGAFVVAPLASFATALAGIALRGT
                     GSSLVWVFSTQLLLEQVPEHLRGRVFATHFAMFQLAGASAALGVGHALDALGYSAVMD
                     ALGGVVLVPGVAFTVWYLARGRRPVAVAVEQPADS"
     gene            8788..9813
                     /locus_tag="VF516_14955"
     CDS             8788..9813
                     /locus_tag="VF516_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8109029.1"
                     /translation="MRGKVIDFLREENRVLREQLGGRRLRFTDDQRRRLAVKGRIVGR
                     RRLGEFAGLVTPDTILRWYRELIARKYDGSARRRFGRPTIATDVEQLVVRMATENPTW
                     GYTRLVGALSNLGHQVGRNTVKRALVRHGLDPAPTRGKRMPWKTFLRTHLGAIAAADF
                     FSVEVLTLTGLVRYFVLFVIDLQTRRVQIGGVVRQAYGAWMTQVARNLTDGVDGFLIG
                     KRHLLHDRDPLFTDEFRGVLAAAGVKCLRLPARSPDLNSVAERFVLSIKSECLSKLVP
                     LGECHLRRAVSEYVEHYHRERNHQGVGNRLLTPAERAVRPANSNAPIERRERLGGLLN
                     FYYRRAA"
     gene            10438..11046
                     /locus_tag="VF516_14960"
     CDS             10438..11046
                     /locus_tag="VF516_14960"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8109030.1"
                     /translation="MVAAANIPAGEYVPTADHRVVLAGATWEHYELELAMRGEKSAPR
                     LAYLEGVLELMSPSRDHERITSYLGRLIEVYAEHADIELSPYRSWTLKAGRTAGGEPD
                     ECYIVGADQSKDRPDLVIEVIWTSGGIDKLALYQRLGIEEVWFWIAGRLEVHALRGDS
                     YERMSRSRWLPGLDLDLMCTFLDRRSVHIAKRDFREALRNAT"
     gene            11069..11371
                     /locus_tag="VF516_14965"
     CDS             11069..11371
                     /locus_tag="VF516_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109031.1"
                     /translation="MHPVASYADKSRGAHESQPKARSRQPIPGRPILVVQIVAPVVQR
                     RVDDNYASRSCQLTARVATMPCEVVLPTRRIARSPESWEITPWVNGVLVQAKGLEF"
     gene            11371..14250
                     /locus_tag="VF516_14970"
     CDS             11371..14250
                     /locus_tag="VF516_14970"
                     /inference="COORDINATES: protein motif:HMM:NF018357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1156 domain-containing protein"
                     /protein_id="HEX8109032.1"
                     /translation="MASSARGPRRLIERDLPIRAISAHARDGRSVHHGHITAIHMWWA
                     RKPLPSCRAVLAAAALPDPVDEACPAEFIAKASEIVSELDQQPPCDRTALRDRLLRLI
                     SDYASWQLAREPGHRQAIRSLIAAAHEAIYGAGPPLLADPFSGGGSIPLEGLRLGASV
                     YASDLNPVAALLGKVTLEYVQRHGTRLLDEVERCGQELDAQAHDYLSRFYPTTDRLPA
                     AYLSFRRIRCEGPGCHADVVLTSKFVLARRGARSIGLVLDGWDGDTPRFKIVEGPPTL
                     FPAPTVRRGAGTCLRCGYTMTVERLREQLGAKQGGSHDALLVAVAVTNVDSTRAFRAP
                     IDADRAAIEAAAQEIARLESESGALPTFPEEKLPPIGTLGFRVQRYGMTRWRDIFTSR
                     QLLTLSTYVRLLRKVLDDDHAVDGLGVAVRSCLALAVDRLADYQNTGCSWNPSSSALP
                     HLFTRQAMPIIWDFGEANPLAESSGSWRGAVEHVLRGLRNALVANHGADVVLASASRH
                     PLPNDCAHLLVTDPPYYDAIPYADLSNFFYVWLRRMLHDDHPSLFGTELVPRDDECIV
                     NPVEGKDRTYYRTVMTQALAEARRVTRPDGVGVVIFAHKSTSGWEDLLAAMLEAGWII
                     TASWPIDTENTMRLRARNSAVLASSIHLVCRPREHANGELITDTVGDWRAILAELPQR
                     IHAWMPRLAAEGVVGADAVFACLGPALELFSRYARVEKVSGEAVTLAEYLEQVWAAVA
                     REALSMIFADADATGLEADARLTAMWLWTLAGGVSAAAANEGDDEEALNEASNDEDGD
                     AQGRYLLEFDAARKIAQGLGARLEELGTLVAIEGPTARLLPVADRARHLLRRSDVAPV
                     RGRKGGARQAEMFAVEQDAAARPESDRLPEPGRTMLDRLHQAMLLFASGRADALRRFL
                     VDTGVGQQAALWRLAQALAALYPSGSDEKRWVEGVLARKRAMGFG"
     gene            14285..>15926
                     /locus_tag="VF516_14975"
     CDS             14285..>15926
                     /locus_tag="VF516_14975"
                     /inference="COORDINATES: protein motif:HMM:NF016358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HEX8109033.1"
                     /translation="MAMKPWYKVVIPREDLRSGRPLDASEFAVHLQHVRDGRAASVYQ
                     DPKEFFERTFLTRTLGEFAIQLLRRLSGIRVETSAVFDLSTQFGGGKTHALTLAYHLV
                     RHGDAAKEWKGVESLLHEAGLTRVPKAATAVFVGTEFDSMVGRGGTDGTPRRLTPWGD
                     LAFQLGGAEAYHLLAQHDVEGIAPGGDVLEKLLPTDQPSLLLLDEVMNYVNRARKSGL
                     ASQLYTFLHNLSEVARARDNVVLAVSIPLAEGEMTVEDHADFQRLKKLLDRLGKAIVM
                     SAEAETSEIIRRRLFEWGGLPKEAIETVDDYAAWFAQNKPLLPSWFPIDKAREELKAC
                     YPFHPSVLSVFERKWQGLPQFQRTRGVLRLLALWVARAYSEGYKGGHKDPLIALGTAP
                     LEDPMFRAAVFDQLGEGRLEVAVTTDIVGKEHAHALRLDAESTAEVKKARLHRKVATS
                     IFFESNGGQQRGESTLPEVRLAVAEPSLDISNVEQCLEALADSCYFLSAENNKVRFSF
                     QANLNKLLADRRASVSPGAIEEYVRAEVQKVLAKGAGVN"
BASE COUNT         2452 a         5626 c         5253 g         2595 t
ORIGIN      
        1 cgacagcacg gccggggccg cgtccacgcc cgcgccggtc gagggcaagc ccgaagcgcg
       61 tccgttcccg aagcagcccg gccccgcgaa gcgcgtcgag gcgatgcccc gcgggcccgc
      121 accgctgcgg atcgacggca agctcgacga gcccgcgtgg gcgaccgcga agccggcggg
      181 cgacttcttc cggcgctggc ccaccgaggg ctggccgagc cagccgacga ccttccgggt
      241 cctctacgac gatcaggcgc tctatttcgg gatcgtctcg atcctcgcac cgggcgagaa
      301 gccgcgcatc accaacctca agcgcgacgc ggtcacgcgg ttctggtacg acgacaacgt
      361 ccagctcgag atcgacgtcg cccacggcaa gcaggatctc atgctgttcg gcgtcaacgc
      421 ggccggcgtc cagctcgacg cgatgcagct cgacaacggg cgccagttcg tgatcgagtg
      481 ggacgcgcag tgggagagcg cctcgcacgt cgagggcaac gtgtggacgg tcgagatcaa
      541 gatcccggcg agcgagctcg gcggcctgtc gcgcctcgcg accatcggga tcaacgtcgt
      601 gcgcaacgac atgatgctcg ccgagaccca ctcgtggagc ctgatcccgc agggcctgcc
      661 gctcgagcac accccgctct acggcgagct gcacggcacc gacgtcggca cgcacacgcc
      721 gctcgtcgcg gaggcctacg tcctcggggc cgcgcccggc ctcgccgaca cgctcaacac
      781 gcacgatcgc gattacggcg ccaaggccgg gctcgatctg cgcacccggc tcggcgcgtc
      841 gacgttcgtc gaggcgtcgg tgctgaccga cttcgcacag gtcgagctcg acaaccagca
      901 gctcaacctc gaccgcttcg cgctgttcta tcccgagaag cgcgacttct tcctgtcggg
      961 cctcgacgtg ttcaacttcg gccgcgccgg catcgcgcag ccgctgttca cgcggacgat
     1021 cgggctcgac gcgaacggcg acatcgagcg ggtgtggggc gggctcaagg cgtaccgccg
     1081 caacgaccgc ttcagcttcg gcctgctcga cgtcgtgacc gccgagaccg cgaactcgcc
     1141 gctgaccaac tgggccgccg gccgcgcgcg cgtccgcgtc ggcgacgcga cgtcgatcgg
     1201 cgtgctcggc acgctcaagc agccgctgac gcaggggacg tcggcgccgg ccgcgaccga
     1261 gaacttcacc gcgggcgccg acttcgagag ccggttcacc gaccgcgcga ccgcctcggg
     1321 cttcggctcg gtcagcgcga cgcccgacac cacgaacccc cacggtgtcc tcgcgggcgg
     1381 cgatctctcg gtcggcgggg acgtgttcgg gccgaagctg agcctgctct atgtctcgag
     1441 gcactacgat agccagacgg gcttcgtcgc gcgcaccgac atcgcgtcga tcgacgggtt
     1501 gctgccggtg cgctacttct tcgcgaacga gcgcatccgc gcgctcgagc tcgacgtgac
     1561 cggcggggtg acgaccgacg ccgcgatccg gcacctcgag accggttacg tcagcggcta
     1621 cgtcaccgcc gagaccaccg gctcgatcaa gccgtacgtg tcgtggaggg ccctgcatga
     1681 cgtcgtgagt gacgacttcg cgctgtcacc cgcgctcacc gtccacgccg gcacgtatcg
     1741 ctcggtcacc ggcgtcatgg gcgtgtccac gacgttcccc gcgatggtgc tgctcgacct
     1801 gtcctacagc cccacgaccg ggttcttcgg cggcgagcgc cagtcgatct ccgagagcct
     1861 gtcggcgccg ctcggccagc acgtgctcgt cgtcgagaac gcgacgggcg cctgggtcaa
     1921 gctcggggac cagcacgact tcctctacgt ggtgaacggc gagctcgatc tccgggcgtc
     1981 caggcaactc gggctcgaga tgctcgcgac gtacgactcg gtcaaggcgg cgttccaggg
     2041 tcaggcccgc ctgcgctggg atccgatcgc gggcaccagc gtgttcctgg tctatcgcga
     2101 cgaccgccgg acggccatga cggccggcac gcgatcgacg acggcggacg accacagcct
     2161 cgtcctcaag gtgacctcgc tgttcaacgt gctctaggag ctcatgcaca tggtacgcat
     2221 ccatcctgtc ctcgcgctcg tggccgccgt gctcgcggca tgccagggcc acgccaccac
     2281 cccgccggcg gccccgccgg cccccagagc ggtacccgcg gccgtcgccg acccgcccgc
     2341 gagtccagat cacctggagc tgatccgggc gctgatgcac gcgccggaga tcgcgttcga
     2401 cggcgccgcg tttcccgagc cgcgcgagcg catccgcgcc tacgtccgcg ccttgctcgc
     2461 cgacgatgcc acctacccgg ggctgctatc ggcgctgtat gcgttcacgc gctacccgat
     2521 gtatccgagc gcggtgccgt tcctggtcca gcacaccgtg atcgacggaa cgccggtgtt
     2581 ctatggccac tacgctcacg acaagggggc gaccggcccc gcatcggccc cgccaccttg
     2641 cccgaagtcc gcggcggtca aggtggtccc gtggtgggat ccatcgaccg aggtcctggt
     2701 gtgtccggac tcgtaccgtc ccaacgtccg gatgttcgcc aaggatcgcg actgcggcac
     2761 gctctccgac ttccagctcc tggtcgccac ccgatcgccg caggagaaca tctgcggctg
     2821 cggtccgaat ctgatctact gcgcacccaa cgaggagatc gactggaaga tccggggagc
     2881 cgcccaggcg gagttcatcg acacgatcgc ctacttcatc aagagcgagc tgccgttcca
     2941 ggacatcctg accacgccag cgacgatgcg ctcgcggatc ggcgacttct tctacctgaa
     3001 gtcgcgcctg ctcgccggcg agcacgtcga ctggaacgag ctccagggcg acgacaaggc
     3061 gatgcgcccc cggccgtggg gcctcggcgg cggcgtgctg tccacgccgc aggtccggtg
     3121 gttcgactgc gccgaccgcg tcgtcatcgc gcacatctgg gaggatttct tgtgcatcgc
     3181 gcaggagtcg aaccgggtcg acaccgagat cctgctccac ggcaccaaga ccgcgaacct
     3241 gcgccgcaac ctcgaccacc tcgccgaggt ccccggctgc cgtgactgcc atgctcggat
     3301 ggagttcggc tcgcggttcc tcgagttctc gaccggcaag gacgacggca cgacgtattc
     3361 ccccgagcgc tcgccgcgcg agagccggat ctacggcaca tcggcggatg acgtgctgtg
     3421 ggaaggccat gccacctcga ccaacctcgg gcgggtgctc agccagcagc cgcagttcgt
     3481 cgattgccag gtcgacaagg tcctcaagcg gttctacggc aacgatccgg tgccgtcggg
     3541 agtgcgggac gagctccgcg accgcttcga tcggtcccgc aacttcgcac agctcctgag
     3601 cgacaccgcc gtcgcccggt tcgcgccctc tgccccgccc cccgcggctc gcgcctggac
     3661 cgagatcgcc gaggatgcgt gcgatgcgtg ccacgacgga tccgatgccc ggcgcaagcg
     3721 catgccgcct gccgaccggc gcgacgccat ccaggccgcg cggcgggtcg ccaattacgc
     3781 catgccgccg agcccggggc ggatggacga tgccgagcgc cggacgctgg ccatgggcct
     3841 gtgccagagc gccgggcttc gcgccggcga gtgcacggac atgctgtatc cgttcgacgc
     3901 cagcgccgac ctgctcccgc tcgacaccct gatcgaacgc atcaccgcgc agcggtccgg
     3961 cgatccgtcc ccggagttca ccgcgatcct caacgagctc aagtccaact tccgcggcga
     4021 gctggccgcg gcggcgcccg acgcgcctcc cgaggacagc ttgaacgtca gcgcgtacct
     4081 gtacctgctc acgaacgatg cctgcgcggc tcggccggac cgcgccacgt gcatcgcaaa
     4141 gctgctcgat gccgaccggc gtcggcgcta gggagatcgc catgcttgac cgacgacaat
     4201 tcctcgcact cggcgcgacg accgcggtcg ccggcgccct gcaccggacg gcgctcgccc
     4261 agcccgccgc ccaggcctcc cttccccacg cccccgtgcg tccgaggacg ttcgtacaga
     4321 tcatcctgcg cggtgggttc gacccgatcc tggcgaccga tcccaaggat cccgagcagg
     4381 tcgcgaggga catcgacgtc ccgtaccgcg atcgcgaggt cgtcgagcgc cgtggccggc
     4441 ggttcggacc ggtgctgcgt ccgctcggcg cgctgctcga cgacctggtg gtggtccgcg
     4501 gcatcaagat ctacaccgtg tcgcacccgg ccgcgatgcg ccgcttgctc gagctccgca
     4561 acgaggtcgc gcgcaacgat cgcctcaccc ccgggttcac cggcgtgctc gatggccagc
     4621 tgccgtggcg cccgatcgga tgcctccggc agctcggcgg aatcgaagca ggcatcggcg
     4681 cagcccgcga cctcagcgtc agatacgagc agatcggtac gctggagcgg ctgcgcaggc
     4741 tcgcgaagac cggtgagcga tgcccgcagg atccgcggct cgacaccttg ctccagcacc
     4801 tggcagccgc gaacgagccg gtcaaggtca ccacgccccg gctcccggcg gtcatcggcc
     4861 gcaactcggc ggaggacacc acgcagacct ggtcgcgtta cctcgccgac ctgttcttcg
     4921 tgctggagaa cgatctcgcc aacaccgtgc tgctctacgc gccatttccg tactgggact
     4981 cgcatcaggc gaaccgcgcc atccagcacg ggtgctgggc ggtgttcgcc ccgatcctgg
     5041 cgacgatcct cggcgagctg cgcgagcgca agagcccgcg aggctgcccg ctcgccgatg
     5101 aggtcgggct cctggtctcg agcgagatcg ggcggtttcc gcggctcaac tcgtacgcgg
     5161 cgggcaagga ccactttccc gagatctcgt gtctgctcag cggcccgggc ctgaaccgga
     5221 ccgcgatcgg cgccaccaac gagcgcatgg ccggcacgat gatcgccgac ggcggtacgc
     5281 gacggttcct ggagctcgac gatctgggcg cctccatcct gcagtggttc ggagtcaccg
     5341 accgcgagcg gctgggctac ctcggccgag gcgtgcccgg cctcctcgcg ggatagccag
     5401 ccatgtcgat ccacgcttcg aacgcccccg ggcggttcgc gcaaggaggc acgacgccat
     5461 gatcgctccc gattccgtca ctgccccggc ggcgtgcgcc ccgagcgcgc gagccggctg
     5521 gctcgccgcc ctcacgatcg ccgccgccgg catgctcgtc gcactgtatc acgccgattc
     5581 gcgcgccctg caccacgacg aggccgcctc gctgcgcaag agccagtact cgtgggacca
     5641 gctcgtccac ccgcgacagt tcgaggacgc accgaccttg ccgaaccagg ccggaccgtc
     5701 ggcgaacacg acctcggagc tgttgaaccg ctggctgttc atcgccgggt tcaaggtgtg
     5761 gaccgatgcg tttggcagct cgccgctcgc gatgcgcggg ttcagcatcg ggttccacgg
     5821 cctgttcgtg ctcgcgttct acgcgctgtt gctcgagctg ctgcgcgagt cctctacccg
     5881 gaccgtgccg cgaccgcgga tcgtcgcgct gatcgcgctc gcgctcggca cgctcaacgc
     5941 gctcgcgctg caccaggcgg gccaggtgcg caactactcg ctgtgcacga cgctgctgat
     6001 gctgaccgca tgggccatct tgcgcgcggc gcggcgcggc cgatggaccg actgggcgct
     6061 ggtcgcggtg ttcgggatcg ccgcgttcta ctcgttcaac gttgcaatga tgacgctcgc
     6121 cggactgtgg acgtggttcg ggttgcgccg cctgttccgc gcccccgcgc gcgccgagtc
     6181 gccggtggcg aggacgagcc acgcgccgtg gatcgcggcg gccgggacga ccgtgctcgc
     6241 gctgctcccc gccgtgctcc acctcgcgtc gcagcgcggc tcgcacggtg cggcgggctg
     6301 gtggatgccg gtgctcacat ggtcgcggtt cttcgagttc gcgccgggct tcgtgttcgg
     6361 cgatagcctg ttcctccaca actcgccggc gaccggcgtc gccatcctcg ctgcgttcgc
     6421 gctcgcgctc taccggacgt ggcgcatcgg gttcctgctc ccggtcgtgg ccgggctcct
     6481 gcccttcctc gcgctcgtcg ccgcgtcgct gaccggcccg aacgtggtga ttccccgcta
     6541 cttcctcgtg ctctcgccct tcctctacac ctgcatggcg atcgcgctgt gcacgatccc
     6601 gtctcaccgg ctccagctcg ccgccgtcgc tggggtgatc gcggtcgtgc tcgcgttcag
     6661 cgtgcgcagc gtgaccacgt gggaccgcca gagcgccggg ggatcgcgcg agatcgcggc
     6721 atggatccag cgcaacgtcg aacccggcgc gaccgtgatc gtgtcgccgt ggctctacct
     6781 cgcggtccgc tactatttcc cgcccgaggc cgacgtccgc gtctacgtgc gcgccgacac
     6841 cgggatcccg ttctacatga gcgggccgtt gctcgaccgc cgcgatcgcg tcacgtcgct
     6901 ctccgacctg atcggcgatc ccccgaaggc acgccacgcc gcctacgtcc acgtcggcat
     6961 caacgagccg ccgatcgatc cggttgcact gcccgagggc tggaccgtcg agcagcgcaa
     7021 gcgattcgag tcgccgttcg tgatctccga ggtcgtggtg gtgagaaccc actagacgat
     7081 ggcgacgttc gagtccgcaa ggatcatgtt cctggtctgc cgcgcgtcgc agccctcgcc
     7141 cccccgtgac cgcgcccgga ggctcgacga tgtccgctaa ccgcccggtc ggcctgctcg
     7201 aggtcgtcgt gaacaaccgc aacttccggc tgctctggct cgcccagctg atcagccagc
     7261 tcggcgattg gttcaacgtc gtcgcgacgg cgactctcgt cggctacctg accggctcgg
     7321 cggcggcgat cggcgtgctg ttcgcggtgc gcctggccgc gccggtgttc gtcagcccgc
     7381 tcgccggcgt gtgcgccgac ctgatgaacc gcaagcacat cctgatcgcc tcggatatcg
     7441 cccgcgcact cacgatgctc ggcttcctgc tgatccgcac gccggacgac atctggctgg
     7501 tctacaccct caccgcgatc cagctcggcc tcagcggcct gttctacccg acgcgcgagg
     7561 cgatcctgcc cgacctcgcc cggggcggcg agctcgtcgc ggcgaacgtg ctcggcatgg
     7621 cctcgtggtc cgtgatggtc tcggtcggca cggcgatcgg cggtgtgatc gccggcgtgt
     7681 gggggccggc ccccgcgttc atgatcgatg cgatgacgtt cgtgatctcc gcgctcctgc
     7741 tcgcgcagat ccgctacgcg ccggtcctgc gcgccgagca cgcacggcct tcggtgcgct
     7801 cggccatcgc gcagtatggc cacagcctcg cctacctccg cgcgaacccc gcgatcttca
     7861 tcgccgtgct ccacaacgtc ttcatcgctg cgttcgccgc gaccacctcg gacatcttcg
     7921 cggcgggcat cgcgcgcgac gtcttcgtct acggcgccgg tggcagcatc tcgctcggta
     7981 tcatgctcgc ctgctccggg ctcgcctcag gcatggcgcc cggcagcttc ctccggacgt
     8041 tcggcggcac gttcgcgcgc aagcaccacg cgatcatcgc gggctacgcg atcatcgcca
     8101 tcggcgcgtt cgtggtcgca cctctcgcga gcttcgccac cgcgctcgcc gggatcgcgc
     8161 tgcgcgggac cggcagctcg ctggtctggg tgttctcgac ccagctcctg ctcgagcagg
     8221 tccccgagca cctgcgcgga cgcgtcttcg cgactcactt cgcgatgttc cagctcgccg
     8281 gcgccagcgc cgcgctcggc gtcggccacg cgctcgacgc gctcggctac tctgcggtga
     8341 tggacgcact cggcggcgtc gtgctcgtcc caggcgtcgc gttcacggtg tggtatctgg
     8401 cgcgcggccg tcgcccggtg gcggtggctg tcgagcagcc ggcggacagc taacacggct
     8461 ctgaggccgg ccaggccgac ttgtcgtcgc gatcagatcg ctggcgtcgt gtgccgaaag
     8521 cccgccggca ggaaatgcga tgcgtattca agcgttgtcg tgctcgtcgc gggccccagg
     8581 ccgatcaccg gcagcgctac catggtgccg cgatgggcac cgaagctgtg cggttcttgg
     8641 tcctgatatg aagtgacctg tcgaggtcta cttggtgttt gtttgaacgt cggtgcgatt
     8701 ctctcctcag tgaggggggc cgtaggcccc cctcactggc gctccagccg cagcgaggcc
     8761 ggagtcaagg ccgcgcagcg ccgagctgtg cgagggaagg tcatcgactt cctgcgcgag
     8821 gagaaccgcg tgctccgcga gcagctcggc ggacggcgcc tacgcttcac cgacgaccag
     8881 cggcggcgcc tggccgtcaa gggccggatc gtcggccgtc gcaggctcgg cgagttcgcg
     8941 gggctggtca cacccgacac gatcctgcgc tggtaccgcg agctgatcgc ccgcaagtac
     9001 gatggctcgg cgcgccgccg gttcgggcgc cctactatcg ccaccgacgt ggagcagctc
     9061 gtcgtccgga tggcgacgga gaatccgacc tggggctaca cgcggctggt cggcgcgctc
     9121 agtaacctcg gccatcaggt cggccgcaac accgtcaagc gcgccctcgt ccggcatggc
     9181 ctcgaccccg cgcccacgcg cggcaagcgg atgccgtgga agacgttcct tcgcacgcac
     9241 ctgggcgcga tcgccgccgc ggacttcttc tcggttgagg tgctcacgct gacggggctc
     9301 gttcgctact tcgtgctgtt cgtgatcgac ctccagaccc ggcgcgtgca gatcggcggc
     9361 gtggtgcggc aggcgtacgg cgcgtggatg acgcaggtcg cacgcaatct caccgacggt
     9421 gtggacgggt ttctgatcgg caagcggcac ctgcttcacg accgggatcc gctgttcacc
     9481 gacgagttcc gcggcgtgct cgcagcggca ggggtgaagt gcctgcgcct ccccgcgcgc
     9541 agtcccgact tgaattccgt ggccgaacgc tttgtgctct cgatcaagtc ggagtgcttg
     9601 agcaagctgg tgccacttgg tgaatgccac ctacggcgtg cggtatcgga gtacgtcgag
     9661 cactatcacc gcgagcgcaa ccaccagggc gtgggcaacc ggctgctcac gccagccgaa
     9721 cgggcggtgc gaccggccaa cagcaacgcg ccgattgagc gccgcgaacg gcttggcggg
     9781 ctgcttaact tctactaccg gcgcgccgca tgagtccgcg atccacttct ggcacacgac
     9841 gcggtgcgcg tggcagccac gtggcagcgg ctccgggggc tacggaagca aatcctatga
     9901 ttctagccag ttaagtcgtg cgcgaagggg gactcgaacc cccacggtgt tacccactag
     9961 cacctcaatc tcaacgaact gcagcaattt tcgctttcta cacgctgacc catacccgcc
    10021 aaggcgattt ccccagcgtg ttcatgcagt tgcgcagtta cccgcgccga ttttcggcct
    10081 ccggtggctt tgcaaagagc ttccgcatcg gcccctcgac tcgcgcccgt cgcgttcgtc
    10141 agccgagctg ctgggccccg cattcacagc gaccgctgat gtcgccgagg tggagccgcc
    10201 agctctcaca aggggaggcg cgatcgccgg aacgcggcgc ctcgcacgca ggcacgatgc
    10261 acgaggcccg tcctgcggcg ctcgcgaccc gcgtcgagat ccgagccgca tgcagcggtg
    10321 agcgcaagcc ttgcgagtgc ccctgccgaa cgcatccagc agggcgcacg atcttcccgt
    10381 cgagcgttcc ggtaggctcg cacccatcgg cagacctcgc atgctagcct tcctgccatg
    10441 gtcgcagcgg cgaacattcc cgccggggag tacgtcccca ccgcggatca ccgcgtcgtc
    10501 ctcgccggtg cgacctggga gcactacgag ctggagctcg cgatgcgcgg cgagaagtcg
    10561 gcgccgcggc tcgcgtacct cgaaggagtg ctggagctga tgagcccgtc gagggatcac
    10621 gaacggatca cgtcgtacct cgggaggctg atcgaggtct acgccgagca cgcggacatc
    10681 gagctgtcgc cgtatcgcag ctggacgctc aaggcgggcc gtaccgccgg cggcgagccc
    10741 gacgagtgct atatcgtggg cgccgaccag tcgaaggacc gccccgacct cgtcatcgag
    10801 gtgatctgga ccagcggcgg tatcgacaag ctcgcgctct accagcgcct cggcatcgaa
    10861 gaggtctggt tctggatcgc cggtcggctc gaggtccatg cgctgcgagg cgacagctac
    10921 gagcgcatgt cgcgcagccg ctggctgccg ggcctcgacc tggacctgat gtgcaccttc
    10981 ctcgaccgcc gctcggtgca catcgccaag cgcgatttcc gcgaggcgct gcgaaacgcg
    11041 acgtaagatc aagtaccgta acggcgggat gcatccggtg gcgagctacg cagacaagtc
    11101 acgaggggca cacgaatctc aacccaaggc gcgctcgcgc cagccgatcc cgggcaggcc
    11161 gatcttggtg gtgcagatcg tcgcgccagt ggtgcagcgg cgtgtagacg acaactacgc
    11221 ctcgagatcc tgccagctga ctgcacgcgt cgcgacgatg ccgtgcgagg tcgttctacc
    11281 gacgcgcaga atcgcgcgat ccccggaaag ttgggaaatc acgccctggg tcaacggcgt
    11341 actggtgcag gcgaaggggc tcgaattctg atggcttcta gcgcgcgagg cccacggcgc
    11401 ctgatcgaga gagatctgcc aattcgcgcc atctcggccc acgcgcgcga tggccgctcg
    11461 gtccaccacg gccacatcac ggcgatccac atgtggtggg cgcgcaaacc actgccgtca
    11521 tgccgtgccg tgttggccgc ggcggcgttg cccgatcctg tcgacgaggc gtgccccgcg
    11581 gagttcatcg cgaaggcctc cgagatcgtc agcgagctcg atcaacaacc accatgcgat
    11641 cgtaccgcgc tccgtgatcg gctcctgcgg ctaatcagtg actacgcctc atggcagctc
    11701 gcgcgcgagc ccggtcacag acaggccatc cggtccttga tcgcggcggc gcacgaggcg
    11761 atctacggtg ctggaccgcc actactcgcg gacccgttct ctggcggcgg atcgatccca
    11821 ctcgaagggc ttcgcttggg cgccagcgtc tatgcgtccg acctgaatcc tgtcgcagcg
    11881 ctgctgggca aggtgactct cgaatacgtg cagcgacacg ggacgcggtt gctcgacgag
    11941 gtggagcgct gcgggcagga gttggacgcg caggcgcacg actatctgtc gaggttctat
    12001 ccgacgaccg atcgcttgcc tgccgcctat ctctcattcc ggcggatccg ttgcgagggg
    12061 ccgggatgcc acgcggacgt cgtgcttaca agcaagttcg tgctggctcg ccgcggcgcg
    12121 cgatcgatcg gcctcgtgct cgacgggtgg gatggcgaca caccgcggtt caaaatcgtc
    12181 gaagggccgc ccaccctgtt cccagctccg acagtgcgcc gcggcgcagg cacatgcctg
    12241 cgatgcggct acacaatgac ggtggagcga ctgcgcgagc aactcggggc caagcaggga
    12301 ggctcgcacg acgcgctgct cgtcgcggtc gctgtcacga acgtcgacag cacgcgcgcg
    12361 tttcgcgcgc cgatcgatgc cgaccgtgct gcgatcgagg cggccgcgca ggagatcgcg
    12421 cggctggaga gcgagagcgg cgcgctgccc acgttccccg aggagaagct accgccgatc
    12481 ggtacgctcg ggtttcgcgt ccagcgctac ggtatgacgc gctggcgcga catcttcacg
    12541 tcgcgccaac tcctgacgct ctcgacctac gtgcgtctgc tgcgtaaggt gctcgacgat
    12601 gaccacgcag tcgatgggct cggtgtcgcg gtccgcagct gcctcgcgct cgctgtagat
    12661 cggctggccg actaccagaa caccggttgc tcctggaacc caagcagctc cgcgcttccg
    12721 cacctcttca ctcgccaggc catgcccatc atctgggact tcggcgaggc gaacccgctc
    12781 gctgagtcat cggggagctg gcgtggcgcc gtcgagcatg tccttcgcgg actgcgcaac
    12841 gcgctcgtcg cgaatcacgg tgccgacgtc gtgctcgcct cggcgagccg gcacccactg
    12901 cctaacgact gcgcgcacct actggtgacc gatccgccat actacgatgc gatcccgtac
    12961 gccgatctct cgaacttctt ctacgtgtgg cttcggcgaa tgttgcacga cgatcatccc
    13021 agcctgttcg gaactgaact cgtgccccgc gacgacgagt gcatcgttaa cccggtcgag
    13081 gggaaggacc gcacctacta tcggaccgtg atgacgcagg cactcgcgga ggcgcggcgc
    13141 gtcacccgcc ccgacggcgt cggggtcgtc atctttgccc acaagtcaac cagtgggtgg
    13201 gaagatctgc ttgcggcgat gctcgaggcc ggatggatca tcacggcatc gtggccgatc
    13261 gataccgaga acacgatgcg ccttcgggca cgcaactcgg ccgtgctcgc ctcctcgatc
    13321 caccttgtat gccgtccgcg tgagcacgcg aacggcgagc tgataaccga tacggtgggc
    13381 gactggcgcg caatcctcgc agagctgccg caacgaatcc acgcgtggat gccacggctc
    13441 gcagccgagg gcgtcgtcgg tgccgatgca gtgttcgcgt gtctcgggcc ggcgcttgag
    13501 ctgttctcgc gctacgctcg cgtcgagaag gtatccggag aggcagtcac cctggcggaa
    13561 tacctcgaac aagtgtgggc cgctgttgcg cgagaagcct tgtcgatgat cttcgcggac
    13621 gccgatgcca cgggcctcga agccgacgcg cgccttacgg cgatgtggct gtggacgctc
    13681 gctggcggag taagcgctgc agcggcgaat gaaggcgacg acgaggaggc gctcaatgag
    13741 gcgagcaacg acgaagatgg cgatgcacag gggcggtacc tgcttgagtt cgatgcggcg
    13801 cgcaagatcg cgcaggggct cggggcacgc ttagaggaac tcggcacgct ggtggcgatc
    13861 gaagggccga ccgctcggct gctgccagtc gccgaccgcg ccaggcactt gctgcggcgg
    13921 agcgacgtcg cgcccgtgcg tgggcggaaa ggtggtgcgc ggcaggcaga gatgttcgcc
    13981 gtggagcaag atgccgccgc gcgccccgag tcggatcgac tccccgagcc cggacggacg
    14041 atgctcgacc gcctgcatca ggcaatgctc ctgttcgcga gcggtcgagc cgatgcgctc
    14101 cgccggttcc ttgtcgacac aggtgtcggc cagcaggctg cgttatggcg gctcgcccag
    14161 gccctcgccg cactgtaccc gagcggctcc gacgaaaaac gctgggtcga gggcgtcctc
    14221 gcacgcaagc gcgctatggg tttcggatga gcgcgcgact ggactccgca gaagagaggt
    14281 acgtatggcg atgaagccct ggtacaaggt cgtcatcccg cgggaggatc tccgcagcgg
    14341 caggccgctt gacgcctctg agttcgcggt ccacctgcag cacgtgcgcg acggccgcgc
    14401 cgcttcagtc taccaggacc ccaaggagtt cttcgagcgc acgttcctca ccagaacgct
    14461 tggtgagttt gcgatccagc tcttgcgccg gctcagcggc atccgcgttg agacctcagc
    14521 ggtcttcgac ctttccaccc agttcggcgg cggcaagacc cacgcgctca cgctcgcgta
    14581 ccacctggtt cgccacggtg acgcggcgaa ggaatggaag ggtgtcgaga gcctcctcca
    14641 cgaagcgggt ctcacacgcg tgccgaaggc agcaaccgcc gtgtttgtcg ggaccgagtt
    14701 cgactcgatg gtcggccgcg gcggcaccga tggcacgccg cggcggctta ccccatgggg
    14761 ggatctcgcg ttccaactcg gcggagccga ggcgtaccat ctcctcgcgc agcacgacgt
    14821 cgagggtatc gcgccgggcg gcgatgtcct cgagaaactc ttgccgacag accagccgtc
    14881 cctgctcctc ctcgacgagg tgatgaacta cgttaatcgc gcgcgcaaga gcgggctcgc
    14941 aagccagctc tacacgttcc tgcacaacct ctccgaggtt gcgcgggcgc gggacaacgt
    15001 tgtgctcgca gtctcgatcc cattggcgga aggtgaaatg acggtcgagg accacgcgga
    15061 tttccagcgc ctcaagaagc tgctcgatcg cctcggcaag gccatcgtga tgtccgccga
    15121 ggcggagacc tcggagatca tccgccgccg attgttcgag tggggaggcc tacccaagga
    15181 agcgatcgaa acagtcgatg actatgccgc gtggttcgcg caaaacaagc cgctgctgcc
    15241 gagctggttt ccgatcgaca aggcgcgcga ggagttgaag gcctgttacc cgtttcaccc
    15301 ctcggttctc tcggtcttcg agcgcaaatg gcagggactg ccgcagttcc agcgcacacg
    15361 tggtgtgcta cgcctgctcg cgctgtgggt ggctcgcgcc tactcggagg gctacaaggg
    15421 cgggcacaag gatccgctga tcgcacttgg gacggcaccc ctcgaggatc cgatgttccg
    15481 tgccgccgta ttcgatcagc tcggggaagg gcggctcgag gtcgcggtga ccaccgatat
    15541 cgtaggtaag gagcacgcgc acgcgcttcg gctcgatgcc gagagtactg ctgaggtgaa
    15601 gaaagcgcgc ctccatcgca aggtcgcaac ctcgatcttc ttcgagtcga acggcgggca
    15661 gcagcgcggc gagtccacgc ttcctgaagt ccggcttgcc gtggccgagc caagcctcga
    15721 cattagcaac gtcgaacagt gcctcgaggc gctggccgac tcctgctact tcctgagtgc
    15781 cgagaacaac aaagtccggt tcagcttcca ggcgaatctc aacaagctgc tggcggaccg
    15841 gcgggcaagc gtgtcccccg gggcgatcga ggagtacgtc cgcgccgagg tgcagaaggt
    15901 gcttgcgaaa ggggccgggg tcaacc
//
LOCUS       DASVOR010000755         1965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_504033,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000755 DASVOR010000000
VERSION     DASVOR010000755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504033"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..627)
                     /locus_tag="VF516_14980"
     CDS             complement(121..627)
                     /locus_tag="VF516_14980"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX8109034.1"
                     /translation="MAADPRTRSIWTIGHSRHPIDRFLALLREHGVEVLVDARSHPAS
                     RFSPQFARRPLERAVGDAGIRYVFLGQALGGRPADPAAYGPDGKLDYDRVERLDAYQH
                     GIDQLLDEAARGRVCVLCAEEDPARCHRRLLIARTLVRRGVRVLHLRGSGAVEPETEL
                     GGGQLALL"
     gene            complement(709..1608)
                     /locus_tag="VF516_14985"
     CDS             complement(709..1608)
                     /locus_tag="VF516_14985"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8109035.1"
                     /translation="MATQQPQAPSREPAKPPFPPQHQDKPGIEAELRPRPRYQAPAYK
                     PAGKLAGKRALVTGGDSGIGRAVAVLYAREGADVAINYLPAEQVDADETKRAIEACGR
                     RCVQYPGDLTDATFCRQLVNCACDELGGLDILVSNAAYQNRKSLAELTPEELDRTFRT
                     NIYAYFFLAQAAIERMPQGSAIIATSSTNAFADPSEMLPDYSATKGAINQLTKVLALQ
                     LVDRNIRVNAVAPGPVWTPLNVADQGMTDEQVEKFGAQTPLQRPAQPDELAPSYVFLA
                     SEGDASYITGIILPVLGGETIGG"
     gene            1703..>1965
                     /locus_tag="VF516_14990"
     CDS             1703..>1965
                     /locus_tag="VF516_14990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109036.1"
                     /translation="MRNPPPQLHLGVPLGGRLMDGRSRGLRGKLACHCLLAEIGDPRI
                     LIGRLALIGQRGSQDAAASGCAGHSLTLGTAPTAAASRAAVRLG"
BASE COUNT          252 a          725 c          701 g          287 t
ORIGIN      
        1 cggctgtgtg gcggcgtgcg gcggcggcgg tactccagcc cgctggcgga cgacgcaccg
       61 tgggcgaccg cggtcttcgg gccgagcaac atctggcagg ccacgatcga cctgtcgcag
      121 ctatagcagc gcgagctggc cgccgccgag ctcggtctcg ggctcgaccg cgccgctgcc
      181 gcggaggtgg agcacccgga ccccgcgccg caccagcgtc cgcgcgatca gcagacggcg
      241 gtggcagcgc gccggatcct cctcggcgca cagcacgcac acccggccgc gcgccgcctc
      301 gtcgagcagc tgatcgatcc cgtgctggta ggcgtcgagc cgctcgaccc gatcataatc
      361 gagcttgccg tccgggccgt aggccgccgg atcggccggc cgcccgccca gcgcctggcc
      421 gaggaacacg tagcggatcc cggcgtcgcc gaccgcgcgc tccagcggcc ggcgtgcgaa
      481 ctgcggcgag aaccggctgg ccgggtggct gcgcgcgtcg accagcacct cgacgccgtg
      541 ctcgcggagc agcgcgagga accgatcgat cgggtgccgg ctgtgtccga tcgtccagat
      601 cgaccgtgtg cggggatcgg ccgccatggc ccaagcatgc cccgaccggc gccgcgcggc
      661 agccggcgag ctactgtcga gacctctccg cctgcccccc gccgggcgtc agccgccgat
      721 cgtctcgccg ccgagcaccg gcaggatgat gcccgtgatg tagctggcat cgccttccga
      781 cgcgaggaac acatacgacg gcgccagctc gtcgggctgc gccgggcgct gcagcggcgt
      841 ctgcgcgccg aacttctcga cctgctcgtc ggtcatgccc tgatcggcga cgttgagcgg
      901 cgtccacacc gggccgggcg cgaccgcatt gacccggatg ttgcggtcga ccagctgcag
      961 cgcgagcacc ttggtcagct ggttgatcgc gcccttggtc gccgagtagt cgggcagcat
     1021 ctccgacggg tcggcgaacg cgttggtcga gctcgtcgcg atgatcgccg agccctgcgg
     1081 catgcgctcg atcgcggcct gtgccaggaa gaagtacgcg tagatgttgg tgcggaacgt
     1141 ccggtcgagc tcttcgggcg tcagctcggc cagcgacttg cggttctggt acgcggcgtt
     1201 ggacaccagg atgtcgagcc cgccgagctc gtcgcacgcg cagttcacca gctggcggca
     1261 gaacgtggcg tcggtcagat cgcccgggta ctggacgcac ctgcgtccgc acgcctcgat
     1321 cgcgcgcttg gtctcgtcgg cgtcaacctg ctcggccggg aggtagttga tcgccacgtc
     1381 ggcgccctcg cgggcgtaca gcaccgcgac cgcgcgtccg atccccgagt cgccgccggt
     1441 cacgagcgcg cgcttgcccg cgagcttgcc ggccggcttg tacgctggcg cctgatagcg
     1501 cggccgggga cgaagctcgg cctcgatccc cggtttgtcc tgatgctggg gcggaaacgg
     1561 cggcttggcc ggttcgcgcg aaggagcctg tggttgttgc gtcgccatat ccggcggtgt
     1621 tgcaagcgcg ccgccagccg cccggcgtgc accatcggcg gatcctcgac gcacgtggcg
     1681 cggcgcttgc tcgaacccga acatgcggaa tccaccaccg caactgcatc tcggcgtgcc
     1741 gctcggcggc cggctgatgg acggtcgctc gcgcggcctg cgcggaaagc tcgcatgcca
     1801 ttgcctgctc gccgagatcg gtgacccgcg gatcctgatc ggccgcctgg ccctgatcgg
     1861 tcagcgcggc agccaggacg ccgcggcctc cggctgcgcc ggccactcgc tcaccctcgg
     1921 gacggccccc accgccgccg cctcgcgcgc cgccgtgcgg ctcgg
//
LOCUS       DASVOR010000756         4886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_36539,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000756 DASVOR010000000
VERSION     DASVOR010000756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4886
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36539"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VF516_14995"
     CDS             complement(<1..653)
                     /locus_tag="VF516_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109037.1"
                     /translation="MQYGGVARERVVDSAGHHAARTARAVPNAIHIAPRGERVRVVGY
                     HGPPTYRRVPDALLVDDRRWYLIALLCRKFPGRNEDVGDVGWIGCRQDETMIQHSLLV
                     LILLTSTAIAQPGTAPPAEQPAPAGTDLDALIAKAQAEAPTIETVVRGTFRRARRALS
                     IGPTVGLWTGAIIGPGDIDAALTIGVGFETFKVPVLPEMETIQDLIVERVKAQLKQR"
     gene            691..1305
                     /locus_tag="VF516_15000"
     CDS             691..1305
                     /locus_tag="VF516_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
                     /protein_id="HEX8109038.1"
                     /translation="MTGSRHHAPATTRNREPIRQVLERVLPPHGTVLEIASGSGEHAI
                     YFARAFPGLTWQSSDASESALASIAAWLAEVQLPNLRPPIALDVTAAVWPVVHADAIV
                     CINMVHIAPWEAALGLFDGAARLLSPGSPIYLYGPYRFGGQFTAASNAEFDRGLRQRD
                     PRWGVRDVDELAAAAGVHGFALEEVVEMPANNHSLLFRAGRAGY"
     gene            complement(1409..2254)
                     /locus_tag="VF516_15005"
     CDS             complement(1409..2254)
                     /locus_tag="VF516_15005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109039.1"
                     /translation="MNLSAMGEVLVFGASKEIEYPFLCTPCCHITMVSDDDNDVDRVL
                     ARLGHCTNQQVQLLKRTSSTRGVTLRVDTGGGRIPLTITMWKMTYGDFRKVAPSKRYD
                     CLIDKASWLYTDPQGWAEYLYKLKVDGYLITDLDYTWEGTAPWVLEVFGLYEVTNSVV
                     GYHRAQHVGYGYDNDSARVYVKVKDVPTSLMHKALLGVAQMDPLLKLYRYPRMNLVGS
                     YLDWDKYQAQANLLRAIAQHARQLDPFRYGSLARRLDQVHQQLTQWIHLREQEDIVAG
                     EHYRQ"
     gene            2760..3689
                     /locus_tag="VF516_15010"
     CDS             2760..3689
                     /locus_tag="VF516_15010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8109040.1"
                     /translation="MIERERKASSAEARGRKVQRASGSEPPVSVILAAVTELEESNRQ
                     FVEAWRLFARAASGGIIESRDGLEIAFAGLALPLVNMMFLSSPVRDAGDLRRRLDAAA
                     TFGRSRGVPWMFTLCEDWLPADAAAEAPAILAAAGLAPMTAATGMVADELAPPRRAAP
                     PALDLRSADGTAAYRELSDLNMIAYSMPVEWGHEAFAREGLFRGGDIWPDIGYLDGTA
                     VSTSTTALVDGRLYVMMVATAADHRNRGYAEAVMRRSLARAEAATGIRRTVLHASPAG
                     APLYAAMGYRSTGRFTMYIVGTGQSSSTSGSAG"
     gene            complement(3727..3897)
                     /locus_tag="VF516_15015"
     CDS             complement(3727..3897)
                     /locus_tag="VF516_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109041.1"
                     /translation="MEALPRARAKEPAVPCHQPPSAVSATRIRGDYKKAIIALNQALC
                     LDSSQRNGSNAA"
BASE COUNT          768 a         1615 c         1683 g          820 t
ORIGIN      
        1 atccgctgct tcagctgcgc cttcacacgc tcgacgatca ggtcctgtat cgtctccatc
       61 tccggcagca ccggcacctt gaaggtctcg aacccgacgc cgatcgtcag cgcggcatcg
      121 atgtcgccgg gaccgatgat cgcccccgtc cacaacccga ccgtcggccc gatcgacagc
      181 gcgcgccgcg cgcgccgaaa cgtgccgcgc acgaccgttt cgatcgtcgg cgcctccgcc
      241 tgcgccttcg cgatcagcgc gtcgaggtcg gtgccggccg gtgccggctg ctccgccggg
      301 ggcgccgtac ccggctgtgc gatcgcggtg ctcgtcaaaa ggatcaggac gagcaagcta
      361 tgttggatca tcgtctcatc ttgccgacag ccgatccatc ctacatcacc gacatcttcg
      421 ttccgtcctg gaaacttccg gcaaagaagc gcgatcaggt accagcgacg gtcgtctacc
      481 agcaatgcat cgggaaccct tcgatatgta ggcggcccgt gatagccgac gacacgtacc
      541 cgctcgccgc gcggcgcgat gtggatcgcg ttcggtaccg cgcgcgcggt gcgcgctgcg
      601 tgatggccgg cgctgtcgac gacacgctcc cgtgcgacgc ccccgtattg cacctcggac
      661 ccgaggcctg ttccgagcta caacgcgtgc atgacgggtt ccaggcatca tgcgccggcc
      721 acgacgcgca accgcgagcc gattcgccag gtgctggagc gtgtgctgcc cccgcacggc
      781 accgtgctgg agatcgcgag cggcagcggc gagcacgcga tctacttcgc gcgcgcgttc
      841 ccgggcctca cctggcagtc gagcgacgca tcggaatcgg cgctcgccag catcgcggcg
      901 tggctcgccg aggtccagct gcccaacctg cgcccaccga tcgcgctcga cgtcaccgcg
      961 gcggtgtggc cggtcgtgca cgccgatgcg atcgtctgca tcaacatggt tcacatcgct
     1021 ccgtgggagg ccgcgctcgg gctattcgac ggtgccgcgc gtctgctgtc gcccggctcg
     1081 ccgatctatc tgtacggtcc gtaccgcttc ggcggccagt tcaccgcggc gtcgaatgcc
     1141 gagttcgatc gcggcctgcg ccagcgcgat ccgcggtggg gcgtgcgcga tgtcgacgag
     1201 ctcgcggccg cggccggagt ccacggcttc gcgctcgagg aggtcgtcga gatgccggcg
     1261 aacaaccatt ccctcctgtt tcgcgccggg cgtgctggct actgatgtca tccggcggga
     1321 tcatggttcg tcacgcccgt gctggctacc ttcagaatcg cccgaggtca ggtagccagc
     1381 acggggctcg ctggcggcct cgtatttttt actggcggta gtgttcgccc gccacgatgt
     1441 cctcctgttc cctcaggtgg atccactgcg tgagctgctg gtggacctga tcgaggcgcc
     1501 tggccagcga cccgtagcgg aaggggtcga gctgacgggc atgttgcgcg atcgcccgaa
     1561 gcaggttggc ctgcgcctgg tacttgtccc agtcgaggta cgagcccacg aggttcatgc
     1621 ggggataccg gtagagcttg agcaggggat ccatctgcgc gacaccgaga agcgccttgt
     1681 gcatgagcga cgtcggcacg tccttgacct tcacgtacac gcgcgcggag tcattgtcgt
     1741 agccgtagcc gacgtgctgc gctcgatggt agcccaccac gctgttggtg acctcgtaca
     1801 ggccgaacac ctcgagcacc cagggtgcgg tgccttccca ggtgtagtcg aggtcggtga
     1861 tgaggtagcc atcgaccttg agcttgtaga ggtattccgc ccagccctga ggatcggtgt
     1921 acagccacga ggccttgtcg atcaggcaat cgtagcgctt cgacggcgca accttccgga
     1981 agtcgccgta cgtcatcttc cacatcgtga tggtcaacgg gatccggcca ccgccggtgt
     2041 ccacccggag cgtgacgccc ctggtggagc tggtgcgctt gagcagctgg acctgctggt
     2101 tggtgcagtg gccgagccgc gcgagcacgc ggtcgacgtc gttgtcgtcg tcgctgacca
     2161 tggtgatgtg acagcacggc gtgcacagaa acgggtattc gatctccttg ctcgctccga
     2221 acaccagcac ctctcccatg gcgctcagat tcatgtactc acggatcgtg agcagcatct
     2281 cgtagcgccc ggcggtcatc gtgggcgggc gcgcgagtgt cggataggac tgcgagttcg
     2341 cgcggcgagg cccgacgggg agcgccagga gcctggaggt cggcgggatt ctggtcggcc
     2401 tgggatcagg tattcggaca atctcgccat tgatgatcac cgtgcggctc atgcgcgatc
     2461 gccattgaac cgcacggcga cgacgcgggc aagaaagcgt ctgcccggtg cgatcggcgc
     2521 gatgcggccg atcggcgact agatcaggta cagcgagtag cccagcgcgg cggcccagct
     2581 cgccgcgcgc tcgatgatcg cgttcgccgg ctgcgcccgc gtcgtcggca gccgctgcac
     2641 gagcacgaac gcgcccgcga tcaggacgcc gcagatcgac agcgtgcgcg cgaacgcctg
     2701 ttcgacgtgc gcatcgagcg cgccgatgaa ccgcgcgagg ctcgcgggga gttctgtggg
     2761 tgatcgagcg cgagcgcaag gcctcctcgg cggaggcgcg agggcggaag gtgcagcgag
     2821 cgtcaggctc ggagccgccg gtgagcgtta tactggccgc cgtgacggag ctcgaagaat
     2881 cgaatcgtca gttcgtggaa gcgtggaggc tgttcgctcg ggctgccagc ggcggcatca
     2941 tcgagagtcg cgatggcctc gagatcgcat tcgccggact ggcgctgccg ctggtgaaca
     3001 tgatgttcct gtcgtcgccg gtgcgcgacg ccggcgactt gcggcgccgg ctcgacgccg
     3061 cggcgacgtt cggccggagc cggggggtgc cgtggatgtt cacgctctgc gaggactggc
     3121 tgcctgcgga tgccgccgcc gaggcgccgg cgatcctcgc cgcggccggg ctggcgccga
     3181 tgacagcagc gacgggcatg gtcgctgacg agctggcacc gccgcgccgg gccgcgccgc
     3241 cggcgctcga cctccgcagc gcggatggca ccgcggcgta ccgcgagctg tccgacctca
     3301 acatgatcgc gtacagcatg cccgtcgagt gggggcacga ggcgttcgct cgcgagggcc
     3361 tgttccgcgg cggcgacatc tggcccgaca tcggctacct cgacggcacg gcggtctcga
     3421 cctcgacgac cgcgctcgtc gacggtcggc tctacgtcat gatggttgcg acggccgccg
     3481 accaccgcaa ccggggatac gccgaggcgg tcatgcgccg atcgctcgcg cgtgcagagg
     3541 ccgcgaccgg catccggcgc acggtcctgc acgcgagccc cgccggcgcg ccgctctacg
     3601 cggcgatggg ctatcgctcg acgggccgct tcaccatgta catcgtgggc accggtcaga
     3661 gctcgagcac gagcggaagc gcggggtaga tgccgcggcc gcgcgctcgg gcgccgtggt
     3721 caccgatcac gcggcgttcg agccgttgcg ctgcgagcta tcgaggcaga gcgcctggtt
     3781 gagggcgatg atcgccttct tgtagtcgcc gcggatccgg gtcgcgctga ccgcgctcgg
     3841 cggctgatgg catgggacgg ctggctcctt ggctctggcc cggggtaacg cctccacgcg
     3901 aagcggttca gccccggata tcgacctcga tcccgtaggc cggtgaacac ggaacggcac
     3961 gcgcacctcg aagccgcagg ccatgctcat gcggtccttg cggtcgagca ggaccggcag
     4021 ccagggcgtg atgtacgtcc actttgcgga ggctcatctc cggccactcc cggagccgat
     4081 cctgagcgcg caacgcggcc acgatgaccc cgacaagagg gtcatcgcga tgatctttgc
     4141 gcgagcgaat tgtgctcagc gtggcagtaa cgtggcagta aaaccgggtc tcccggtcga
     4201 taatacttta atttcagcta gttaaagggc ggagcggacg ggacttgaac ccgacaggcg
     4261 gcaagccaag atcgtcaacc attacgcgat tctgtagtca gcacgctggc ttgcgcgccg
     4321 ttggtcgtcc cgcctcgtcc cttctgatcc cgcctgatcc cgatgcgtgt tgcagcaagc
     4381 ttgcagcacg aatccgcgga gtatctgggt ggcctgttgg aaatgaaatc ggatatcaaa
     4441 atcgaaaaca accagcgatg gggcaaacct cgggggcggc cggcagtcag cggctagcgc
     4501 ggcggagcag gttgggaagg cggtgaccgg gcgggcggcg atctggcgag ccggttgcgg
     4561 aaggcgcgcc gctcgctgct ggcgctgggg ggctcgctgc ggacgtaatc cgatgggccg
     4621 aactcggccc gagccaagcg cgcgccggct gccgccgcac cgcatcgtcg gcatgctcga
     4681 ccgcgacttc accaaggcgc cggaatcacc ccgaggccag ctacgcaacc gtcgagagcg
     4741 cggtgctgcg tgagatgacc gagcggacca tgttctcgat ctgcgacgac aagacccggt
     4801 ttcacctcaa cggcgtgttc ttcgagagcg gcggctcgaa gtgccagatg gtgtcgaccg
     4861 acggacaccg gctgggatgt cgagct
//
LOCUS       DASVOR010000757         6470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_270087,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000757 DASVOR010000000
VERSION     DASVOR010000757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270087"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1021)
                     /locus_tag="VF516_15020"
     CDS             complement(<1..1021)
                     /locus_tag="VF516_15020"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109042.1"
                     /translation="MADLDLSGRTLGEFVLREQIGAGGYGAVYRCEQPVLKRHAVVKV
                     LHAGRRRNNTAKERFLREAQLASRLDHPYAAHVYSFGAEDGGLLWIAMELVQGITLGD
                     LLKSRGPMPLDEFVPFFECVAQVVQAAHTRGIVHRDLKPSNMMVIESGGRLFPKLLDF
                     GIAKLNDDSEAAEAHDDEDEPALGGPVDRTATVRIRVTPQRVQRTRTDSKPAARLTRR
                     GAVIGSAPYMSPEQWGDPCAVGPATDIYSLGCVAYEALTGHAPFTAANTDEYYKQHLR
                     ADPPSLGNAFSPDLDRVLQRALAKAPEARHASALELAAEFRAVLRAQPREQLRSAAQH
                     WQDRAR"
     gene            complement(1259..2029)
                     /locus_tag="VF516_15025"
     CDS             complement(1259..2029)
                     /locus_tag="VF516_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109043.1"
                     /translation="MPREHDLREFCCRLFGWSDDHASLVDDVMWTLLSAVARRTAIGL
                     QGESDLVPIAHSLHCRLVGAERPFVVCDPRRRDSDGSVRAPPSRKAGMPALDAAIGGS
                     LCLRSNRLPDDFDLLAASFHESGSAALFVCLHSNDRITDLLCRPLKIPPLTERLPEFG
                     RLLNEYLDEAAQILGARHVRLSERMQQSVLHHVRSLSDLEKAALRLVALQSAGNLYRA
                     AHQLHMAPISLIRWMTRRGWTPGALRGTDERDGGADDR"
     gene            2440..3543
                     /locus_tag="VF516_15030"
     CDS             2440..3543
                     /locus_tag="VF516_15030"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8109044.1"
                     /translation="MVELETSRTKTDSAGPNGWSICDEVTRLREWGTDIVYPLPSRRE
                     TSIIGASGDCWLRLWDPTGRISRRHAALSYGVDGWSLSDLQSKNGVHLDGARVASLSL
                     VPGAEIRIGPVTLIAESPRLIALRQLLERIIGWGEERREEVDQALFTIRIAATHREPL
                     LLCGAGNLVPIARSLHHHALGNQPFIVCKPRTPYAQGMDALAAATGGTLCIWRNQQPD
                     DFDDVVSALRGPAARALLVVCAHALPRGNDIASQIVTVVRSILIPPLADRARELNRII
                     DAYFADAISAFGGGWVTAADREWVANNASSSLHQIGMATRRIVALHACDESVTHASKL
                     LAMSHGSLSDWLARRSLPGRSQMSDEEDDADDR"
     gene            complement(3562..4527)
                     /locus_tag="VF516_15035"
     CDS             complement(3562..4527)
                     /locus_tag="VF516_15035"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipase family protein"
                     /protein_id="HEX8109045.1"
                     /translation="MDSSKTPSDSISQIPVDPSLTLALAQASVAAYADYEGKPVVPPP
                     GYRFLDRWTGWDSFVLGGQEERFGVLFRSIASPPTYIFAFRGTDSDLDIWNDLFAETT
                     TFVPYDSSKTPQPPAYVSAGFYGIYSLTGGSMQRSMQQQVFALLQKYGIDFFYITGHS
                     LGAALSSLFTLDVRLTTPHLRPSNMNFASPMVGTERWAQVYAQQGAPTRRVFNYYDYA
                     PDLPPLPYYTHVGTSFQCAFYEYNEWYPHLLNRHRMLNLQTVLNNAVWRTPQIWIGYF
                     PDAVDPSVTMISVPPPVGERPGTVAALREVKRREHAPAEAAAPHH"
     gene            4972..6072
                     /locus_tag="VF516_15040"
     CDS             4972..6072
                     /locus_tag="VF516_15040"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8109046.1"
                     /translation="MTTRREFLANAVGLAGVCFVGCSLLGPHRAHAQPRRTVKVGGKR
                     VKVIDVHAHAVVPEALELAGQKLGEGIWRPEVAMPANADQRIAAMDAQGIDVEALSIN
                     AFWYASEREVAAKICKLQNERLAEVCGKYPDRFVAYASVALQYPELAADQLEQGIKKY
                     GLRGAAIGGSCAGKELSDPMFHPFWAKAEALGAPVFIHPQPSGAPTELKQRFQGNGYL
                     ENVVGNPLETTIALSHLIFGGSLDRFPRVKIIAAHGGGFLPSYSGRADHGCLSRPASC
                     PGGAFGPIKKKPSEYLRQMYFDTMVFTAEGLRHLAAEVGASQLVVGTDSPYPWTTTAV
                     DHVLTTPTLSDADKLAILQGTAAKLLNISTQA"
     gene            6081..>6470
                     /locus_tag="VF516_15045"
     CDS             6081..>6470
                     /locus_tag="VF516_15045"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8109047.1"
                     /translation="MSHPSHHARIHPDKIAYRMARSGDAISYRELDARSNQGAQLFRA
                     LGLQAGDHIALVMENRLEFMAICWAAQRSGLYYTTISSYLTRDEVAYIVADCGARLVI
                     ASPRYGDSLGGVDRDAPGVPALSMVGEP"
BASE COUNT         1007 a         2172 c         2302 g          989 t
ORIGIN      
        1 gcctggcgcg gtcctgccag tgctgcgccg ccgaccggag ctgctcgcgc ggctgggcgc
       61 gcagcacggc gcggaactcg gcggcgagct ccagcgcgct ggcatggcgg gcctcgggcg
      121 ccttggccag ggcgcgctgc aggacgcgat cgaggtccgg cgagaacgcg ttgccgagcg
      181 agggtgggtc ggcgcgcagg tgctgcttgt agtactcgtc ggtgttcgcc gcggtgaacg
      241 gcgcgtggcc ggtcagcgcc tcgtaggcga cgcagcccag cgagtagata tcggtcgccg
      301 ggccgacggc gcacggatcg ccccactgct cgggggacat gtacggtgcc gagccgatca
      361 ccgccccgcg ccgggtcagg cgtgcggccg gcttggaatc ggtgcgggtc cgttgcacgc
      421 gctgcggggt cacgcgaatg cgcaccgtcg ccgtgcgatc gacgggcccg ccgagggccg
      481 gctcgtcctc gtcgtcgtgc gcctcggcgg cttcgctgtc gtcgttcagc ttggcgatcc
      541 cgaagtcgag caacttgggg aacaggcgac cgccgctctc gatcaccatc atgttcgatg
      601 gcttcaggtc gcggtgcacg atgccgcgcg tgtgggcggc ttgcacgacc tgggcgacgc
      661 actcgaagaa cggcacgaac tcgtcgagcg gcatcggccc gcgcgacttc agcagatcgc
      721 cgagcgtgat gccctggacc agctccatcg cgatccacag caggccgccg tcctcggcgc
      781 cgaacgagta gacgtgggcc gcgtagggat ggtcgaggcg agacgccagc tgggcctcgc
      841 gcaggaaccg ctccttcgcg gtgttgttac gccgccgccc cgcgtgcagg accttgacga
      901 cggcgtgacg tttgagcacc ggttgctcgc agcggtacac ggcgccatag ccacccgcgc
      961 cgatctgctc gcgcagcacg aactcgccca gcgtacgccc tgagaggtca aggtctgcca
     1021 tcggctccgc cgcaaggcta gacacagcac atgcgcaggg atactgtggc agatgcagca
     1081 acttgctgca tctttcagaa cttgcggtgc ggaaaggtgc gatggcgtcg tacgcgtggc
     1141 ggctatgccc cgtgtcgagg cttgcagctt cgatgcgtcc cggaccgccg catgcttgcg
     1201 caacctctac gggcctgtgg gttgctgcgc tgcgccggcc gcctgcggca tgccgaggtc
     1261 agcggtcgtc ggcgccgcca tcgcgttcgt ccgtgccgcg cagtgcgccg ggcgtccatc
     1321 cgcggcgggt catccagcgg atcagcgaga tcggcgccat gtggagctga tgggccgctc
     1381 gatacaggtt ccccgcgctc tggagcgcca cgaggcgcag cgccgccttc tcgaggtccg
     1441 acagcgatct gacatgatgc agaacggact gctgcatgcg ctccgagagc cggacatggc
     1501 gtgcgccgag gatctgcgcg gcctcgtcga ggtactcgtt cagcaggcgc ccgaactccg
     1561 gcaggcgctc ggtcagcggc gggatcttga gcgggcggca cagcaggtcg gtgatgcggt
     1621 cattgctgtg caggcacacg aacagcgccg ccgacccgga ttcgtgaaac gacgccgcca
     1681 gcaggtcgaa gtcgtcgggc agccggttgg accgcagaca cagcgagccg ccgatcgccg
     1741 catcgagcgc cggcatcccg gccttgcggc tcggcggagc ccgcacggag ccatcgctgt
     1801 cgcggcgccg cggatcgcag accacgaacg gtcgctccgc gccgaccagt cggcagtgca
     1861 gcgagtgggc gatcggcacc aggtccgatt caccctgcaa tccgatcgcg gtccgccgcg
     1921 cgacggcgct gagcagggtc cacatcacat catcgacgag gctggcgtgg tcatcggacc
     1981 agccgaacag gcgacagcag aactcccgaa gatcgtgctc gcgtggcatg gcggtgccgc
     2041 tttcctctcc gatcgaatgc ggttccagga aacgacggtt cacgttcgaa gattctgggt
     2101 ctgaagcaac gatattcctc gacatcgtga ggtttcgcga catgaggctc tgtggggatg
     2161 gaagatggga atgcatcatg tgcttcgcac tgcatcatcc gcgctgaacc gcactctctg
     2221 ctacccggcg gcgccgctgg tcgattggcc ggctcagggc tgcaaacgcg accgcagcat
     2281 attgctcgat ccacacggcg tggccagacg gcgtggggcg gccggcgcgg ggccggctgc
     2341 agcgtcggac ggcaccgggc tccgtcggtt tcgcggctca ggatgcaaaa ccagaccggg
     2401 cgttcgcggg cgacggaacg agcgtatgct ccggggccca tggtcgagct agagacatcc
     2461 cggaccaaga cggactcggc gggcccgaac ggctggagca tctgcgatga agtgacccgg
     2521 ctgcgtgagt ggggaaccga catcgtctac ccgctgccat cgcggcgcga gacctccatc
     2581 atcggtgctt ccggggactg ctggctgcgg ctgtgggacc cgaccgggcg gatctcgcgg
     2641 cggcatgccg cgctgagcta cggcgtggac ggctggtcgc tctcggacct ccagagcaag
     2701 aacggcgtcc acctcgacgg tgcccgcgtg gcctcgctgt cgctggtgcc cggcgccgag
     2761 atccgcatcg gaccggtcac cctgatcgcc gagagcccga ggctcatcgc gctgcgccag
     2821 ctgctcgagc ggatcatcgg ctggggcgag gagcgccggg aggaggtcga ccaggcgctg
     2881 ttcacgatcc gcatcgccgc cacccaccgc gagccgctgc tgttgtgcgg tgcggggaac
     2941 ctggtaccga tcgcccggtc actgcatcat cacgcgctgg gcaatcagcc attcatcgtc
     3001 tgcaagccca ggactccgta cgcccagggc atggacgcgc tggccgccgc gaccggaggc
     3061 acgctgtgca tctggcggaa tcagcagccc gacgacttcg atgacgtggt gtcggcgctg
     3121 cgcgggcccg ccgctcgcgc cctgctcgtg gtgtgtgccc acgccttgcc gcgcgggaac
     3181 gacatcgcct cgcagatcgt cacggtcgtg cgctcgatcc tcatcccgcc gctcgccgat
     3241 cgggcccgcg agctcaatcg catcatcgat gcctacttcg cggatgcgat ctccgccttt
     3301 ggcggcgggt gggtcaccgc ggcagatcgc gaatgggtcg cgaacaacgc atcttcctcc
     3361 ttgcatcaga tcgggatggc gacgcgtcgc atcgtcgcgc tgcatgcctg cgacgagagc
     3421 gtgacgcacg cttcgaagct gctcgcgatg tcgcacggct cgctgtcgga ctggctcgct
     3481 cgccgatcgc tccccggcag gtcgcagatg tccgacgagg aggacgacgc ggacgaccgc
     3541 tagcaccgcc ggctgccgcg ctcagtggtg cggcgcggcg gcctccgcgg gcgcgtgctc
     3601 gcgccgcttc acctcgcgca gcgctgcgac ggtgccgggg cgctcgccga cgggcggcgg
     3661 aacgctgatc atcgtgaccg aggggtcgac ggcgtccggg aagtagccga tccagatctg
     3721 gggggtgcgc cacaccgcgt tgttgagcac ggtctgcagg ttgagcatgc ggtgccggtt
     3781 caggagatgg ggataccact cgttgtactc gtagaaggca cactggaacg atgtgcccac
     3841 gtgcgtgtag tacggaagcg gcggcagatc gggagcgtag tcatagtagt tgaagacccg
     3901 ccgggtcggc gcaccctgct gggcgtagac ctgtgcccag cgctcggtgc cgaccatggg
     3961 gctcgcgaag ttcatgttgc tcgggcgcag gtgaggtgtg gtcagcctga catcgagggt
     4021 gaacagcgat gacagcgcgg cgcccaggct gtggccggtg atgtagaaga agtcgatgcc
     4081 gtacttctgc agcagggcaa acacctgctg ctgcatcgac cgctgcatcg acccgccggt
     4141 gagggagtag atgccgtaga accccgcgct cacgtaggcc ggaggctgcg gggtcttgct
     4201 gctgtcgtac ggcacgaacg tggtggtctc ggcgaacagg tcgttccaga tgtcgagatc
     4261 cgagtcggtg ccgcgaaacg cgaagatgta ggtcggcgga ctggcgatcg agcgaaacag
     4321 cacgccgaac cgctcttcct ggccgccgag cacgaagctg tcccacccgg tccagcgatc
     4381 caggaaccgg tagcccgggg gagggaccac cggtttgccc tcgtagtcgg cgtaggccgc
     4441 caccgacgcc tgtgcgagcg ccagcgtcag gcttggatcg acggggatct gcgagatcga
     4501 gtcggacggt gtcttcgagc tgtccatgag tgcctccgtt agacgccaca gttcacgcac
     4561 cgcgcccgcc ggcccgcggg ggcgggtcac ggtcgcgaca cgccgacgac gctagtcccc
     4621 gcaacgtgag aagacaatta ccggcgatta cagcagatca cggcgggcgg tccagcgccg
     4681 cgcgatcgtg gaccgccgtg tggacgagcc agcgcgcttc gccggtgcat ggcgcaagcg
     4741 tctgctcgtc cgctgcctcg cctcgctcgc ctgcacgctg ctcaccggcc gcggccgatg
     4801 attctgctga ccgtgcgcgg ctttgcccag gtccggatcg gacggaacac ggctgtcgcc
     4861 gcacgcgacc agctgtgaga ggatgccgcg cacgctcgcg gccggcggca gcgccgcggc
     4921 gggatcccca gccaccgcaa ccgcatcgcc gcagcttcag gaggattcga tatgacgacg
     4981 cgaagagagt tcctggccaa cgcggtcggt ctcgccggcg tgtgcttcgt gggctgcagc
     5041 ctcctcgggc cgcaccgcgc gcacgcccag ccccggcgca ccgtcaaggt cggcggcaag
     5101 cgcgtcaagg tgatcgacgt ccatgcccac gcggtggtgc ccgaggcgct cgagctcgcc
     5161 gggcagaagc tcggcgaggg gatctggcgg ccggaggtcg cgatgccggc gaacgcggac
     5221 cagcggatcg cggcgatgga cgcccagggc atcgacgtcg aggcgctcag catcaacgcg
     5281 ttctggtacg cgtccgagcg cgaggtcgcg gcgaagatct gcaagctcca gaacgagcgg
     5341 ctcgccgagg tctgcggcaa gtacccggat cgcttcgtcg cctacgcgtc ggtcgcgctc
     5401 cagtacccgg agctggcggc cgaccagctc gagcagggga tcaagaagta cggcctgcgc
     5461 ggcgccgcga tcggcggcag ctgcgcgggc aaggagctca gcgatccgat gttccacccg
     5521 ttctgggcca aggcggaggc gctcggcgcg ccggtgttca tccacccgca gccgagcggc
     5581 gcgccgaccg agctcaagca gcggttccag ggcaatggct acctggagaa cgtggtcggc
     5641 aatccgctgg agaccacgat cgcgctgtcg cacctgatct tcggtggatc gctcgaccgg
     5701 ttcccgcggg tgaagatcat cgcggcgcac ggcggcgggt tcctgccgtc atactcgggg
     5761 cgcgccgatc acggctgcct gtcgcggccg gcgagctgcc cgggcggcgc gttcgggccg
     5821 atcaagaaga agccgagcga gtacctgagg cagatgtact tcgacacgat ggtgttcacg
     5881 gccgaggggc tgcgccacct ggccgccgag gtcggcgcga gccagctcgt ggtcggcacc
     5941 gactcgccgt acccgtggac gacgaccgcg gtcgaccacg tactgaccac gccgaccctg
     6001 tccgacgccg acaagctggc gatcctgcag ggcacggcgg cgaagttgct caacatcagc
     6061 acgcaggcct gacccgggcg atgtcgcacc catcgcacca cgcccggatc caccccgaca
     6121 agatcgcgta ccggatggcg agatccggcg acgcgatcag ctatcgcgag ctcgacgcgc
     6181 ggtcgaacca gggcgcgcag ctgttccgcg cgctcggcct gcaggccggc gatcacatcg
     6241 cgctggtgat ggagaaccgc ctggagttca tggcgatctg ctgggcggcg cagcgcagcg
     6301 ggctgtacta cacgacgatc agcagctacc tgacccgcga cgaggtcgcc tacatcgtgg
     6361 ccgattgcgg tgcgcggctc gtgatcgcgt cgccccggta cggcgactcg ctcggcggcg
     6421 tcgatcgcga cgcgcccggc gtgccggcgc tgtccatggt cggcgagccg
//
LOCUS       DASVOR010000758         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_241009,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000758 DASVOR010000000
VERSION     DASVOR010000758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241009"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2201)
                     /locus_tag="VF516_15050"
     CDS             complement(<1..>2201)
                     /locus_tag="VF516_15050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109048.1"
                     /translation="AGNAIYAVSNVEGRYDSVQTYDAGILSFGIMQWTLHAGSLQKFL
                     GFLKDKSGPEGRAAFQDDFAAKGIDVKPNGGQYQLWYNGKEYPLGADGEGKAAIDQLV
                     RQDKPTARRWAEIFAAAGADARVQKAEFERAKEMYQETQGIKFTDKVVDQSLKACKKT
                     FHAKYRDQYGKAEAWMTASPKAGALFFSMRVNNPKYANAAFLKAIDAFYDAHGTDRAR
                     WPANWGEDFGNQVEAKCKETLDTWHSEGGNEGRVEKTLRFWNQAQNQAGAPAATNAAP
                     AAKPAAPAVAAAPANKDPAADGKLDAARTQKLRAQYAAIMKQFVRGKIDQGEAVKSLI
                     AYDEKLNGGKPSVEGLVLLPLLLADLVKATLARSSAAPAPAPAPAPAKHEVTKPAQAE
                     VKPPDSITDVKQPPAAARPPVAPANVDAAPPHVAGKAISKILKLPRPLPQVHAFLPPG
                     GAKGNVEVFLFLHGMFAYHDRSQQKGFRPGKDINDPNPDQAMNLAGAMAATARNLVTL
                     APVAHFDGEWPLWNELAENKGFKELIFQSVDQLSSDLSITPALGVGSVSIAGHSGGGS
                     GLGAAAKQLGDLVHDMTYEDAGYADKPGKSGWKDSHDKVAAWLLGGDSDKVLRVMLHG
                     PHNHLESTILHSHFNKESLEHTAQVLGKHGVTVTREEGNHDKRTIDGTTYLDHKLHVS
                     GLPGTRTVSVFVLPSEDHMALRNLSTQHLITEGRDTEFAAGAAPAGGPLKA"
BASE COUNT          251 a          746 c          792 g          412 t
ORIGIN      
        1 ccgccttgag cgggccgccg gccggggcgg cgccggccgc gaactcggtg tcgcggccct
       61 cggtgatcag gtgctgggtc gacaggttgc gcagcgccat gtggtcttcg ctgggcagga
      121 cgaacaccga cacggtgcgc gtccccggca gaccgctcac gtggagcttg tggtcgaggt
      181 acgtggtccc gtcgatggtg cgcttgtcgt ggttgccctc ctcgcgcgtc acggtgacgc
      241 cgtgcttccc gagcacctgc gcggtgtgct cgagcgactc cttgttgaag tgcgagtgca
      301 ggatcgtgct ctccaggtgg ttgtgcgggc cgtgcagcat cacgcgcagc accttgtcgg
      361 agtcgccgcc gagcagccag gccgcgacct tgtcgtgcga gtccttccag cccgacttgc
      421 ccggcttgtc ggcgtagccg gcgtcctcgt acgtcatgtc gtggaccagg tcgccgagtt
      481 gcttggcggc tgcgccgagc cccgagccgc cgccgctgtg accggcgatc gagaccgagc
      541 cgacgccgag cgccggcgtg atcgacaggt cgctgctgag ctgatcgacc gactggaaga
      601 tcagctcctt gaaccccttg ttctcggcga gctcgttcca cagcggccac tcgccgtcga
      661 agtgcgcgac cggcgcgagg gtcaccaggt tgcgcgcggt cgccgccatc gcgccggcga
      721 ggttcatcgc ctggtcgggg ttgggatcgt tgatgtcctt gccggggcgg aagccctttt
      781 gctggctgcg gtcgtgatag gcgaacatgc cgtggaggaa caggaacacc tcgacgttgc
      841 ccttggcgcc gccgggtggc aggaacgcgt ggacctgcgg cagcggccgg ggcagcttca
      901 ggatcttcga gatcgccttg ccggcgacgt gcggcggcgc ggcatccacg ttcgccggag
      961 cgaccggcgg cctggcggcg gccgggggtt gcttgacgtc ggtgatgctg tccggcggct
     1021 tgacctcggc ctgcgcgggc ttggtcacct cgtgcttggc tggcgccggt gcgggggccg
     1081 gcgccggtgc cgcggagctg cgcgccagcg tggccttcac caggtcggcg agcagcagcg
     1141 gcagcagcac cagcccctcg accgacggct tgccgccgtt cagcttctcg tcgtaggcga
     1201 tcaggctctt gaccgcctcg ccctgatcga tcttgccgcg cacgaactgc ttcatgatcg
     1261 cggcgtactg cgcgcgcagc ttctgggtcc gggcggcgtc gagcttgcca tcggccgcgg
     1321 gatccttgtt cgccggcgcg gcggcgaccg ccggggccgc gggcttggcc gcgggagccg
     1381 cgttcgtcgc ggccggtgcg ccggcctggt tctgcgcctg gttccagaac cgcagcgtct
     1441 tctcgacccg gccctcgttg ccgccctcgc tgtgccaggt gtcgagcgtc tccttgcact
     1501 tggcctcgac ctggttgccg aagtcctcgc cccagttcgc cggccacctg gcgcggtcgg
     1561 tgccgtgcgc gtcgtagaac gcgtcgatcg ccttgaggaa ggccgcgttg gcgtacttgg
     1621 ggttgttgac ccgcatcgag aagaacagcg cgccggcctt cgggctcgcc gtcatccagg
     1681 cctcggcctt gccgtactgg tcgcggtact tggcgtggaa cgtcttcttg caggccttga
     1741 gcgactggtc gacgaccttg tcggtgaact tgatgccctg ggtctcctgg tacatctcct
     1801 tggcgcgctc gaactcggcc ttctggaccc gcgcgtcggc gccggccgcg gcgaagatct
     1861 ccgcccaccg ccgcgcggtc ggcttgtcct ggcgcaccag ctggtcgatc gcagctttgc
     1921 cctcgccgtc ggcgccgagc gggtactcct tgccgttgta ccagagctgg tactggccgc
     1981 cgttcggctt gacgtcgatg cccttggcgg cgaagtcgtc ctggaacgcg gcgcggccct
     2041 cggggccgga cttgtccttc aggaagccga ggaacttctg caggctgccg gcgtgcagcg
     2101 tccactgcat gatgccgaac gacaggatgc cggcgtcgta ggtctggacc gagtcgtagc
     2161 ggccctcgac gttggacacc gcgtagatcg cgttgccggc g
//
LOCUS       DASVOR010000759         2497 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_358044,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000759 DASVOR010000000
VERSION     DASVOR010000759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358044"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..488
                     /locus_tag="VF516_15055"
     CDS             <1..488
                     /locus_tag="VF516_15055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109049.1"
                     /translation="QAAAREPDGRVDLTPRSLRIEPAELEHLKQLNDLVASPRAAKRF
                     INLYRMIRSMLDDEALDRLITGGYKITQVCLAVVIGSPSRGAELFEAILSGRVTSRAE
                     LASWCTERAAKVDGTRDRLTLRYLARRDSEFTDWNMVSDAVRRVARFSFETGSVLRHY
                     S"
     gene            698..1732
                     /locus_tag="VF516_15060"
     CDS             698..1732
                     /locus_tag="VF516_15060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109050.1"
                     /translation="MTGWTHVRAGSARLSWIAAAVLAATLPLAQGCATQGAGDPASGE
                     EEPTGEMVLSLTQPGPHGEIYQLTNANFDISNAQGTFTINGNGFSSQLSVPMPPGVAS
                     IFLRDGWTLGKSTDGGVTFQPVSALLGSFNPIGVRVLANQPAFVRFDFLIRQTNGNLQ
                     LSFGVVTDPRELAGGFVVQTATGALADYALPQNQRMDFAVFFQLSSVESVTLADGTKQ
                     RIYTSFNSGSSFGPIPVPDGALAAEFYSDHLGILSGPVASDLTGATLTYTVAAHPDGS
                     ITVSGDLISGNTELTFAPDAIDAVLPPLDADGFPADAFFYDSDVPCTLDFGPDTITGT
                     LRVRNLPPAP"
     gene            complement(1742..>2497)
                     /locus_tag="VF516_15065"
     CDS             complement(1742..>2497)
                     /locus_tag="VF516_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109051.1"
                     /translation="HARDAPALAVAAATDIAFRQFTYNNNLTPARQRDDREGGEVLTG
                     ATVELWPTTLLHLGALPGLSLYGRFQIGVNPQPVTIRDSVTGAVMPTALTTSWRSLEV
                     SLHQRWTLANTGTVEVGAGYTSDRYQFTTDGTPTGDAQRAMVPDAAYKAVRIGGRASL
                     LLGRLEPYLTAENRLVLSGGQMEHRYSVSTSVNGVQGALGAVVHLGRFEVRAEASLLL
                     YSWSFRYDNGDDDKADGGSDQIERIGLTVGYVY"
BASE COUNT          363 a          877 c          873 g          384 t
ORIGIN      
        1 cgcaggcggc cgcgcgcgag ccggacggcc gggtcgatct gacgccgcgc agcctgcgga
       61 tcgagccggc cgagctcgag cacctgaagc agctcaacga cctcgtcgcg tcgccgcgcg
      121 cggccaagcg gttcatcaac ctgtaccgga tgatccggtc gatgctcgat gacgaggcgc
      181 tcgatcgcct gatcaccggc ggctacaaga tcacgcaggt ctgcctcgcg gtcgtgatcg
      241 gcagcccgtc gcgcggcgcc gagctgttcg aggcgatcct gtccgggcgc gtgacgtcgc
      301 gggccgagct ggcgagctgg tgcacggagc gagccgccaa ggtcgacggc acgcgcgacc
      361 gcctcacgct gcgctacctc gcccggcgag acagcgagtt cacagactgg aacatggtca
      421 gtgatgccgt gcgccgggtg gcgcggttct cgttcgagac cggaagcgtg ttgcgccatt
      481 acagctgatc cggatcacag ctggcggagc aatcttccgc ttgttgccgc atgagccccg
      541 cgcgcgactc cgtatcttga gtcttctata cgtaaccagc gcacagaccc cggggtccgt
      601 gcaccggcta cgcaagacgg cgcgcgggag acggccatgt cggccgtcgg cgcgtcgcct
      661 tgccaacaag gagcttgtga tgatctgcag tgaatcgatg accgggtgga cacacgttcg
      721 agcgggcagc gcgcggctgt cgtggatcgc cgccgctgtg ctcgcggcca cgctaccgct
      781 ggcccagggc tgcgccacgc agggcgccgg cgatcccgcc tccggtgagg aggagccgac
      841 gggcgagatg gtgctctcgc tgacccagcc ggggccgcac ggcgagatct accagctgac
      901 caacgcgaac ttcgacatca gcaatgcgca ggggacgttc acgatcaacg gcaacggctt
      961 cagttcccag ctgagcgtgc cgatgccacc cggcgtcgcg tcgatctttc tgcgcgacgg
     1021 ctggacgctg ggcaagtcga ccgacggcgg cgtgacgttt cagccggtgt cggcgctgct
     1081 cggcagcttc aaccccatcg gggtccgcgt cctggccaac cagccggcgt tcgtccggtt
     1141 cgacttcctg atccggcaga ccaacggcaa cctgcagctc tcgtttggtg tcgtgaccga
     1201 cccgcgcgag ctcgcggggg gcttcgtcgt ccagaccgcg accggcgcgc tggcggatta
     1261 cgcgctgccg cagaaccagc ggatggactt cgcggtgttc ttccagctgt cgtcggtgga
     1321 gtcggtgacg ctggccgacg gcaccaagca gcgcatctac acctcgttca acagcggcag
     1381 ctcgttcggc ccgatcccgg tgccggacgg cgcgctcgcc gcggagttct acagcgacca
     1441 cctcggcatc ctgtcgggac cggtcgcgag cgatctgacc ggcgcgaccc tgacctacac
     1501 ggtcgccgcc cacccggacg gcagcatcac ggtgtccggc gatctgatca gcggcaacac
     1561 cgagctcacg ttcgctcccg acgcgatcga cgcggtgctg cccccgctcg acgccgacgg
     1621 gttcccggcc gacgcgttct tctacgactc ggacgtcccg tgcaccctgg acttcgggcc
     1681 ggacacgatc accggcacgc tgcgggtccg caacctcccg ccggcgccgt gacccgcgcc
     1741 gtcagtacac gtagccgacg gtgagcccga tgcgctcgat ctggtcgctg ccaccgtcgg
     1801 ccttgtcgtc gtcgccgttg tcgtagcgga acgaccagct gtagagcagc aggctggcct
     1861 cggcgcggac ctcgaaccgg ccgaggtgga ccaccgcgcc gagcgcgccc tgcacaccgt
     1921 tgaccgaggt gctcaccgag tagcgatgct ccatctggcc gcccgacagc accaggcggt
     1981 tctcggcggt gaggtagggc tcgagccggc cgagcagcag cgacgcgcgg ccgccgatcc
     2041 gcacggcctt gtaggcggcg tccggcacca tggcgcgctg cgcgtcgccg gtcggcgtcc
     2101 cgtcggtggt gaactggtag cgatcgctgg tgtagccggc gccgacctcg accgttcccg
     2161 tgttcgcgag cgtccagcgc tggtgcaggc tgacctccag gctgcgccac gacgtggtga
     2221 gcgcggtcgg catcaccgcg ccggtcaccg agtcccggat cgtgacgggc tgcgggttga
     2281 ccccgatctg gaaccggccg tagagcgaca ggccgggcag ggcgccgagg tggagcagcg
     2341 tggtgggcca cagctcgacg gtcgcgccgg tcagcacctc gccaccctcg cggtcgtcgc
     2401 gctgccgcgc cggggtcagg ttgttgttgt aggtgaactg gcggaacgcg atgtcggtcg
     2461 cggcggccac cgcgagcgcc ggggcgtcgc gcgcgtg
//
LOCUS       DASVOR010000760         1872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_299786,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000760 DASVOR010000000
VERSION     DASVOR010000760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1872
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299786"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..705)
                     /locus_tag="VF516_15070"
     CDS             complement(<1..705)
                     /locus_tag="VF516_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109052.1"
                     /translation="MRSAIFAACLICGCGRIDFAPLTGDAFVGSGGGVGGGDPGDAGG
                     VPSDVAPAGACGGVVLLADDFEDGAIAPVWTELTGTNLTVAETAGFLQITYGSNVPAS
                     QAAGYITTSNLDFTGTCVDVELAMAPNPATLASAEVRIGTPTNCSVFDVTNGQLNGAQ
                     HRGASISRMPAIPYDPVAQRHLRLREAAGVWYFEISPDGSAYTTIYTVMNSFALQTST
                     TLRLVASSASAVNSGGV"
     gene            complement(919..>1872)
                     /locus_tag="VF516_15075"
     CDS             complement(919..>1872)
                     /locus_tag="VF516_15075"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8109053.1"
                     /translation="PPPLPPHGKPAEVVGTEHWTTRLAGDAKVRLFLWRKRLRSPAAG
                     EGRQGTILFVHGSSISSTPTFDLQVPGRPELSAMDWFARKGYDTWCVDCEGYGRSDKA
                     RPINADVSNGADDLVAASDYIMKQTGGDKLLLYGESSGGLRAALHAQRNPEQVKRLAV
                     DAFVWTGEGSPTLAERRKRLPQFRASNRRPIDRDFIRSIFTRDHPGTSDQTIVEAFAD
                     AALARDTSVPTGTYIDMCANLPLVDPEKLSVPVLILRGQYDGIAALDDVMRFFARLPD
                     PDKQFVMMPGIAHSSMRAKNFQIIYHALDAYFSQPAPIYVG"
BASE COUNT          279 a          634 c          676 g          283 t
ORIGIN      
        1 gacgccgccg ctgttgaccg cgctcgccga gctcgcgacc agccgcaacg tggtcgacgt
       61 ctgaagcgcg aacgagttca tcaccgtgta gatcgtcgtg tacgcactgc cgtccgggga
      121 gatctcgaag taccagacgc cagcggcctc gcgcaggcgc aggtgtcgct gggcgaccgg
      181 gtcgtagggg atcgccggca tcctgctgat cgaggcgccg cgatgctgcg cgccgttcag
      241 ctgcccgttc gtgacgtcga acacgctgca gttggtgggc gtgccgatcc ggacctcggc
      301 gctcgccagg gtcgcggggt ttggcgccat cgcgagctcg acgtcgacgc aggtcccggt
      361 gaagtcgagg ttgctcgtcg tgatgtaacc ggccgcctgg ctcgcgggca cgttgctgcc
      421 gtacgtgatc tggagaaagc cggcggtctc ggccacggtc aggttggtcc ccgtcagctc
      481 ggtccacacc ggcgcgatcg cgccgtcctc gaagtcgtcg gccagcaaca ccaccccacc
      541 gcacgcaccg gcgggcgcga cgtcagaggg cacgcccccg gcatcgccgg ggtcaccgcc
      601 gccaaccccg ccaccgctgc cgacgaacgc gtcacccgtg agcggcgcga aatcgatgcg
      661 cccacagccg cagatcaggc atgccgcgaa gatggccgac cgcacccttc cgagcatatc
      721 tcgcctggtc cggcggtgcg gacttcccgg ccggtctgcg acagcgggta agcacctgcg
      781 acgagcaacg gcaacgcttg gatacgcatc gcatttccag ccggcggacg ttcaccacat
      841 gacgaggtcg cggcgaggac cgcggcgcgc gccacggcga ccgtcgtcac gaggatgaca
      901 gcaggtagct ccgcatgtct aaccgacgta gatcggcgcc ggctggctga agtacgcgtc
      961 gagcgcgtga tagatgatct ggaagttctt cgcgcgcatg ctggagtgcg cgatgccggg
     1021 catcatcacg aactgcttgt cgggatcggg cagcctggcg aagaaccgca tgacgtcgtc
     1081 gagcgcggcg atgccgtcgt actggccgcg caggatcagc accggcacgc tcagcttctc
     1141 ggggtcgacc agcggcaggt tggcgcacat gtcgatatag gtgccggtcg gcacgctggt
     1201 gtcgcgcgcg agcgcggcgt cggcgaacgc ctcgacgatg gtctggtccg aggtgccggg
     1261 gtgatcgcgc gtgaagatgc tgcggatgaa gtcgcggtcg atcggccggc ggttgctggc
     1321 gcggaactgc ggcaggcgct tgcgccgctc ggccaaggtc gggctgccct cgccggtcca
     1381 cacgaacgca tcgaccgcga gccgcttgac ctgctcgggg ttgcgctggg cgtggagcgc
     1441 ggcgcgcagc ccgccggacg actcgccgta cagcaagagc ttgtcgccgc cggtctgctt
     1501 catgatgtag tcggacgcgg cgaccaggtc gtcggcgccg ttggacacgt cggcgttgat
     1561 cggccgcgcc ttgtccgagc gcccgtagcc ctcgcagtcg acgcaccagg tgtcgtagcc
     1621 cttgcgggcg aaccagtcca tcgccgacag ctcgggccgg ccggggacct ggaggtcgaa
     1681 ggtcggcgtc gacgagatcg acgagccgtg gacgaacagg atcgtgccct gccggccctc
     1741 gcccgccgcg gggctgcgca gccgcttgcg ccacaggaac agccggacct tggcgtcgcc
     1801 ggcgagccgg gtcgtccagt gctcggtgcc gacgacctcg gcgggcttgc cgtgcggtgg
     1861 cagcggcggc gg
//
LOCUS       DASVOR010000761         2013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_328785,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000761 DASVOR010000000
VERSION     DASVOR010000761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328785"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VF516_15080"
     CDS             <1..902
                     /locus_tag="VF516_15080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109054.1"
                     /translation="RAPLWTAVAVAALVAAAAGSAAASPTQDLDRARQAFREHDYESA
                     AKLATYLLYPEERLGLPVDLVEAHVILGASDFEIGHRAEAKREFERALQIQPEKVLTD
                     MLFSEGAIRLFDETKADIEARARRDAELRKIADERERIRKYRDSLVVVERRSFGVNFL
                     PFGAGQFQNKQPTRGLVFAASEGLTGGLSVGIFLYLSGKYGLNASVKPPEVIQVRQLE
                     QIEIGAGIAFFAIYALGVVDSLLHYKPRVQIEGDDSLLPLPSDVPDTRKPRPGKTSLL
                     DRVHLAPIAAPGGAGIGLIWEND"
     gene            902..>2013
                     /locus_tag="VF516_15085"
     CDS             902..>2013
                     /locus_tag="VF516_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8109055.1"
                     /translation="MPSLRLTAPGHPPTVYNLYKKITSLGSGPDNDIVLPDPLVVDGF
                     AVHFDGQTYTVLAPRKAEFVVNGKKRGKHKLSHDDRLVLGSCELKFAMIDEAPPLEEE
                     AAETIAELDAYTKLYEFSERLIHQRDLGELLEALMDAVIEITNADKGFLVLLEGDTID
                     VKVARNLNRENIADAVSQLSDSIIAKVVRSRKPVIVSDAMRDDEFSAAKSVMQLKVSS
                     VICVPLLDRGRLLGLIYVGNDAIRDLFQQDTLRILTVFASQASLIIANALLLNELRID
                     NKRLNERLEQYRFGEIVGTSPPMQQVFRKVEKIAATDISVLITGETGTGKELIAREIH
                     NRSPRAGKPFVTINCGAIPENLLESELFGHVKGAFTG"
BASE COUNT          342 a          721 c          661 g          289 t
ORIGIN      
        1 gccgcgcgcc gctgtggacc gccgtggccg tcgccgcact ggtcgccgcc gccgcgggca
       61 gcgccgccgc atcgccgacc caggacctcg accgggcgcg ccaggcgttc cgcgagcacg
      121 actacgagtc ggcggccaag ctcgcgacct acctgctcta ccccgaggag cggctcgggc
      181 tgccggtcga tctggtcgag gcccacgtga tcctcggcgc gagcgacttc gagatcggcc
      241 accgcgccga ggccaagcgc gagttcgagc gcgcgctgca gatccagccc gagaaggtgc
      301 tgaccgacat gctgttctcc gagggcgcga tccggctgtt cgacgagacc aaggccgaca
      361 tcgaggcccg ggcccggcgc gacgccgagc tgcgcaagat cgccgacgag cgcgagcgga
      421 tccgcaagta ccgcgacagc ctggtcgtgg tcgagcgccg gtcgttcggg gtcaacttcc
      481 tcccgttcgg cgccggccag ttccagaaca agcagccgac ccgcggcctg gtgttcgccg
      541 ccagcgaggg cctgaccggc gggctgtcgg tcggcatctt cctctacctg tccggcaagt
      601 acgggctcaa cgcctcggtc aaaccgcccg aggtcatcca agtccgccag ctcgagcaga
      661 tcgagatcgg cgcgggcatc gcgttcttcg cgatctacgc gctgggcgtg gtcgactcgc
      721 tcctgcacta caagccgcgg gtccagatcg agggcgacga ctcgctgctg ccgctgccca
      781 gcgacgtgcc cgacacccgg aagccgcgcc cgggcaagac ctcgctgctc gaccgcgtcc
      841 acctcgctcc gatcgccgcg ccgggcggcg ccggcatcgg actgatctgg gagaacgact
      901 gatgccttcg cttcgcctca ccgcacccgg gcacccgccc acggtctaca acctctacaa
      961 gaagatcacc tcgctcggct cgggacccga caacgacatc gtgctgcccg atccgctggt
     1021 ggtcgacggc ttcgcggtgc acttcgacgg ccagacctac accgtgctcg cgcccaggaa
     1081 ggccgagttc gtggtcaacg gcaagaagcg cggcaagcac aagctgtcgc acgacgaccg
     1141 cctggtgctc ggcagctgcg agctcaagtt cgcgatgatc gacgaggcgc cgccgctcga
     1201 ggaggaggcc gccgagacca tcgccgagct cgacgcctac accaagctct acgagttctc
     1261 ggagcggctg atccaccagc gcgacctcgg cgagctgctc gaggcgctga tggacgcggt
     1321 catcgagatc accaacgccg acaagggctt cctggtcctg ctcgagggcg acacgatcga
     1381 cgtcaaggtc gcgcgcaact tgaaccgcga gaacatcgcc gacgcggtgt cgcagctgtc
     1441 ggactcgatc atcgccaagg tcgtgcgatc gcgcaagccg gtgatcgtgt ccgatgcgat
     1501 gcgcgacgac gagttctcgg cggccaagag cgtgatgcag ctcaaggtgt cgagcgtgat
     1561 ctgcgtgccg ctgctcgacc gcggccggct gctcggcctg atctacgtcg gcaacgacgc
     1621 gatccgcgac ctgttccagc aggacacgct gcggatcctc acggtgttcg cgtcgcaggc
     1681 ctcgctgatc atcgccaacg cgctgctgct caacgagctg cggatcgaca acaagcggct
     1741 caacgagcgg ctcgagcagt accggttcgg cgagatcgtc gggaccagcc cgccgatgca
     1801 gcaggtgttc cgcaaggtcg agaagatcgc cgcgaccgac atcagcgtgc tgatcaccgg
     1861 cgagaccggc accggcaagg agctgatcgc gcgcgagatc cacaaccgct cgccgcgggc
     1921 cggcaagccg ttcgtcacga tcaactgcgg cgcgatcccc gagaacctgc tcgagtccga
     1981 gctgttcggc cacgtcaagg gcgcgttcac cgg
//
LOCUS       DASVOR010000762         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_212105,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000762 DASVOR010000000
VERSION     DASVOR010000762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212105"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1732)
                     /locus_tag="VF516_15090"
     CDS             complement(56..1732)
                     /locus_tag="VF516_15090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109056.1"
                     /translation="MKTSRIQMFALVLAAGCAASPEWPPAQFANAPVVWRVDDRRNVP
                     VPPAERPTYLNFYNYNGVVLRPLTRAMELPPPHRALGVNALDDVPDSTWFTNRIGVRD
                     MTIDEVRTGPNLVGSPELHRPWTVHRTKPGTPEVGLFITDARGVEFLLKFDLPGHPEE
                     ETAAHVLAGKLLWACGQHVTDDSVVQFGPRDLVPAPDAVVEDVFGNTHPLGRAELDDI
                     LRSTQTTPDGRLRAVASRWLPGKSLGGIPSEGVRADDPNDRIPHEMRRDLRGAYPMVA
                     WVDHVDMQEGNFIDSWIEDGGRHYVVHHMIDFGKALGVMNTTKLNPRPGFEYDLDLGA
                     MAHWLGTLGIVRRPWEGQNPPPLIGVGMLGVEHFDPDGWVPGTQAYLPLLIADRFDKF
                     WGAKIVIRFSPAQIRAAVEAGRLSDPRAVDYLTATLVARQRATARYWFDQVNPLDHFV
                     IVPHDGGAELCFDDLALAYRLGADPAATRYHLMSYDRNGNPIGAATAFRPTGAGGHAC
                     IGPLTLATGGDRYTIFRVETTRPDVALATLVYAALDPRTSAPRVIGIYRL"
     gene            complement(1729..>3141)
                     /locus_tag="VF516_15095"
     CDS             complement(1729..>3141)
                     /locus_tag="VF516_15095"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8109057.1"
                     /translation="LLSLYGDYEKHPKDIFYGIGNHGETPEPPPANRELTAADVAGAP
                     QPGNESGRLDVEDRDSTARRALRGVLYVPKVLVEVVLAPFQLPVWAYDRYHLKELYYR
                     VFYNEDRTIGVIPTASYATEYGFTAGAKFLATDLAGEQELLWVSAQFGGTYQQAYQAY
                     LDSGDRLGDHLLLSLYGDYEKHPKDIFYGIGNHGETPEPPFPVNPYLVPVTVKTRFRQ
                     RIERFVGTINWRMLDDLYLVSSNEVTDRHFSPATTGEPINEIYNTSTLVGWPSTKYFY
                     TELALRYDTRGRYNLFEPAAFYSLGTLVEAWGGFNHALNDLADYWRYGLNFQHFFRLA
                     ESPRVLMLRFHGEAVSAGLSDVPFDMLPKLGGPRDLRGYTPDRFRDRIAWVGTVEYSW
                     DLSNYVAAALFTDVGRVYPSLDDITYKHMRAGFGVSFRFYGPNSFWLDATLATSADGG
                     FQATASFDPIYFIRPRVRRR"
BASE COUNT          480 a         1017 c         1152 g          492 t
ORIGIN      
        1 gcccgctacc agccgtatta ctacggttcg accctggtct atcgaccgat gtagctcaca
       61 gccgatagat cccgatcacg cgcggtgcgc tggtcctggg atcgagggcg gcgtacacga
      121 gggtcgccag cgcgacgtcg ggccgcgtgg tctcgacccg gaagatggtg tagcgatcgc
      181 cgccggtcgc cagggtcagc gggccgatgc aggcatgacc gccggcgccg gtcgggcgga
      241 acgccgtggc cgccccgatc gggttcccgt tccggtcgta cgacatcagg tggtagcgcg
      301 tcgcggccgg gtccgcgccg agccggtagg ccagcgccag gtcgtcgaag cacagctcgg
      361 caccgccgtc gtgcggcacg atcacgaagt ggtcgagcgg gttgacctga tcgaaccagt
      421 agcgcgcggt ggcgcgctgg cgcgcgacca gggtggcggt caggtagtcg accgcgcgcg
      481 gatcgctcag ccgtccggcc tcgaccgcgg cgcggatctg cgcgggcgag aaccggatca
      541 cgatcttcgc gccccagaac ttgtcgaacc ggtcggcgat cagcagcggc aggtaggcct
      601 gcgtgccggg cacccagccg tcggggtcga agtgctcgac gccgagcatc ccgaccccga
      661 tcagcggtgg cgggttctgg ccctcccacg gccggcggac gatccccagc gtgcccagcc
      721 agtgcgccat cgcgccgagg tcgaggtcgt actcgaagcc gggccgcggg ttgagcttgg
      781 tcgtgttcat cacgccgagc gccttgccga aatcgatcat gtggtgcacg acgtagtggc
      841 gaccgccgtc ctcgatccac gagtcgatga agttgccctc ctgcatgtcg acgtgatcga
      901 cccatgcgac catcgggtac gcgccgcgca ggtcgcggcg catctcgtgc gggatgcggt
      961 cgttggggtc gtccgcgcgc acgccttcgc tggggatccc gccgagcgac ttgccgggca
     1021 gccagcgcga cgccaccgcg cggagccggc cgtcgggagt ggtctgcgtg ctgcggagga
     1081 tgtcgtcgag ctcggcgcgg ccgagcgggt gcgtgttgcc gaacacgtcc tcgacgaccg
     1141 cgtcgggcgc gggcacgagg tcgcgggggc cgaactgcac gacggagtcg tcggtgacgt
     1201 gttgcccgca ggcccacagc agcttgccgg cgagcacgtg cgccgccgtc tcttcttcgg
     1261 ggtggcccgg gagatcgaac ttgagcagga actcgacgcc gcgggcatct gtgatgaaca
     1321 ggccgacctc cggggtgccg ggcttggtgc ggtgcacggt ccacggccgg tgcagctcgg
     1381 ggctgccgac caggttgggg ccggtgcgca cctcgtcgat cgtcatgtcg cgcacgccga
     1441 tccggttggt gaaccaggtc gagtcgggca cgtcatcgag cgcgttgacc ccgagcgcgc
     1501 gatgcggcgg cggcagctcc atcgcgcggg tcagcgggcg caggaccacg ccattgtagt
     1561 tgtagaagtt caggtaggtc gggcgctcgg ccggcgggac cggcacgttg cggcgatcgt
     1621 cgacccgcca gaccaccggg gcgttggcga actgcgcggg cggccactcc gggctcgcgg
     1681 cgcagccggc cgcgagcacg agcgcaaaca tctggatccg ggaagtcttc atcgtctcct
     1741 cacgcgcggt ctgatgaaat agatgggatc gaaggacgcc gtcgcctgga agccgccgtc
     1801 ggccgacgtg gccagggtgg cgtcgagcca gaagctgttc gggccgtaga accggaacga
     1861 gacgccgaag ccggcgcgca tgtgcttgta cgtgatgtcg tcgagcgacg ggtacacacg
     1921 accgacgtcg gtgaacagcg cggccgcgac gtagttcgac agatcccacg agtactcgac
     1981 cgtgccgacc cacgcgatgc ggtcgcggaa ccggtcgggc gtgtagccgc gcagatcgcg
     2041 tggcccgccg agcttgggca gcatgtcgaa cggcacgtcg gacagcccgg cggagaccgc
     2101 ctcgccgtgg aaccggagca tgagcacgcg cggactctcg gcgagccgga agaagtgctg
     2161 gaaattgagg ccgtagcgcc agtagtcggc gaggtcgttg agcgcgtggt tgaagccacc
     2221 ccacgcctcg acgagcgtgc ccagcgagta gaacgccgcg ggctcgaaca ggttgtagcg
     2281 gccgcgggtg tcgtaccgga gcgcgagctc ggtgtagaag tacttcgtgc tcggccagcc
     2341 gaccagcgtc gacgtgttgt agatctcgtt gatcggctcg ccggtggtcg ccggcgagaa
     2401 gtggcgatcg gtcacctcgt tcgagctcac gaggtagagg tcgtcgagca tgcgccagtt
     2461 gatcgtcccc acgaaccgct cgatgcgctg gcgaaaacgg gtcttgaccg tgaccggaac
     2521 cagatagggg ttgaccggga atggcggctc cggcgtctcg ccgtggttcc cgatgccgta
     2581 gaagatgtcc ttcgggtgct tctcgtagtc gccgtagagc gagagcagga gatgatcgcc
     2641 gaggcggtcg ccgctgtcca ggtacgcctg gtacgcctgc tggtacgttc cgccgaactg
     2701 ggccgatacc cacagcagct cctgctcgcc tgccaggtcg gtagccagga acttcgcgcc
     2761 ggcggtgaat ccgtactccg tcgcgtacga cgccgtcggg atcacgccga tcgtgcggtc
     2821 ctcgttgtag aacacccggt aatagagctc cttgaggtgg tagcggtcgt acgcccacac
     2881 cggcagctga aacggcgcca gcacgacctc gacgaggacc ttgggtacat agagcacgcc
     2941 gcgcagcgca cgccgcgcgg tcgagtcgcg gtcctccacg tcgagccggc cgctctcgtt
     3001 cccgggctgg ggcgcgccgg cgacgtcggc cgcggtgagc tcgcggttcg ccggtggcgg
     3061 ctccggcgtc tcgccgtggt tcccgatgcc gtagaagatg tccttcgggt gcttctcgta
     3121 gtcgccgtag agcgagagca g
//
LOCUS       DASVOR010000763         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_445549,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000763 DASVOR010000000
VERSION     DASVOR010000763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445549"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VF516_15100"
     CDS             <1..982
                     /locus_tag="VF516_15100"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8109058.1"
                     /translation="LEVAVAERDAAQQARVVREGELLALRTSSRELAGELERLTDVVH
                     RDEVARAEQRLRIEQLETAAVEQYGVDLDALVAEYGPEELVPPSAADLASADADGKPQ
                     PEPAPYERAVQEKRAARAERDLALLGKVNPLALEEFAALEERHAFLSTQLEDLKATRR
                     DLLTVIREVDERIHDVFASAYADTAREFEIVFRTLFPGGEGRLVLTDPEDMLATGIEV
                     EARPPGKKIKRLSLLSGGERSLTAVALLVAIFRARPSPFYVLDEVEAALDDVNLGRLL
                     NLIEELRSTSQLIVISHQKRTMEIADALYGVSMRGDGITTVISQRLRESA"
     gene            1076..1330
                     /locus_tag="VF516_15105"
     CDS             1076..1330
                     /locus_tag="VF516_15105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109059.1"
                     /translation="MPAIQIRDVPPDLYRRLRERAAADRVTLSAYVLRLLERDAGRPS
                     AQEWFAALADREPVRDADVAGALDGSRSDRDTRAARVLRH"
     gene            1314..1700
                     /locus_tag="VF516_15110"
     CDS             1314..1700
                     /locus_tag="VF516_15110"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8109060.1"
                     /translation="MYCVIDAGALLELLLRTLRAESVQSVVDDRQMVAPAVIDAEVLS
                     ALRGLERSRAVTPARAALAVADLREAPVERYPLGSLLDRAWGLRSRLNAYDALYVALA
                     VELDCPLVTTDDRIPSPRLPAKVLHA"
BASE COUNT          264 a          707 c          753 g          276 t
ORIGIN      
        1 gctcgaggtc gcggtcgccg agcgggacgc cgcccagcag gccagggtgg tccgcgaggg
       61 cgagctgctc gcgctgcgca cctcgagccg cgagctggcc ggcgagctcg agcggctcac
      121 cgacgtcgtg caccgggacg aggtggcccg ggccgagcag cggctgcgga tcgagcagtt
      181 ggagaccgcg gcggtcgagc agtacggggt ggacctcgac gcgctggtcg ccgagtacgg
      241 acccgaggag ctggtgccgc cgtcggccgc cgacctggcc agcgccgacg cggacgggaa
      301 gccacagccc gagccggccc cgtacgagcg ggcggtgcag gagaagcggg ccgcccgggc
      361 cgagcgcgac ctggccctgc tgggcaaggt caacccgctc gcgctggagg agttcgcggc
      421 gctggaggag cggcacgcgt tcctgtccac ccagctggag gacctgaagg cgacccggcg
      481 cgacctgctc acggtgatcc gcgaggtcga cgagcgcatc cacgacgtgt tcgcctcggc
      541 gtacgcggac accgcgcggg agttcgagat cgtcttccgc acgctgttcc cgggcggcga
      601 aggccggctg gtgctcaccg acccggagga catgctcgcc accggcatcg aggtcgaggc
      661 caggccgccg ggcaagaaga tcaagcggct gtcgctgctg tccggcggcg agcgctcgct
      721 gaccgcggtc gcgctgctgg tcgcgatctt ccgggcccgc ccgtcgccgt tctacgtgct
      781 ggacgaggtc gaggccgcgc tcgacgacgt caacctcggc cggctgctca acctgatcga
      841 ggagctgcgc tcgacctcgc agctgatcgt catcagccac cagaagcgga cgatggagat
      901 cgccgacgcg ctgtacgggg tgagcatgcg cggcgacggc atcaccacgg tgatcagcca
      961 gcggctgcgc gagtccgcct gaggacgcac ggcctgggcc gaccgtcgca acttctgccg
     1021 gccgatcgca gcaaagtttt cggagcgcac caccgcggcg ctagcatctg atgccgtgcc
     1081 tgccatccag atccgggacg tgccgcccga tctgtaccgc cgactgcgcg agcgcgcggc
     1141 ggcggatcgg gtgacgctct ctgcgtacgt gctgaggctg ctcgagcggg acgcgggtcg
     1201 cccgtcggcg caggagtggt tcgccgccct cgccgaccgg gagccggtgc gtgacgcgga
     1261 cgtagcgggg gctctggacg ggtcgcggtc cgaccgcgac acgcgggccg cccgtgtact
     1321 gcgtcattga cgccggggcg ctgctcgaac tcctgctgcg cacgctccgc gccgagtcgg
     1381 tgcagtccgt ggtcgacgac cggcagatgg tggcgccggc ggtgatcgac gcggaggtgc
     1441 tctccgcgct gcgcggcctc gaacgcagcc gcgccgtcac gccggcccgg gccgcgctgg
     1501 ccgtcgccga cctgcgcgag gcgccggtcg agcgctaccc gcttggctcg ctgctggacc
     1561 gggcctgggg cctgcgcagc cggctcaacg cgtacgacgc gctctacgtc gcgctcgctg
     1621 tcgagctgga ctgcccgctg gtcaccaccg acgaccgcat ccccagcccc cgcctccccg
     1681 ccaaggtcct gcacgcctga ctccatcggg ggcggccccc gtaacccccg ggcggtggct
     1741 cgggtgctct ggtgagtctt cgaccgacct cttccaacga gccccgccga tggcggaggg
     1801 ccccatcatg actactaccc gccgggttag gtgccggctc tcctgagagg atcgacggca
     1861 tggagtgggt gatcctcgcg gtggcgatcg tgatcgtgct cgtgattgcg attgcgggtc
     1921 tggtggtgcc gcggcggcgg gggacgaggt tgccgccggc gccggagaag gcggttccgc
     1981 cgctgggggc cgacaccgag
//
LOCUS       DASVOR010000764         2822 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_7821,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000764 DASVOR010000000
VERSION     DASVOR010000764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2822
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7821"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2822
                     /locus_tag="VF516_15115"
     CDS             <1..>2822
                     /locus_tag="VF516_15115"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX8109061.1"
                     /translation="ATAVAPASPPPPPEDGAATAHAASPFPIVGIGASAGGLEALDQF
                     LRNIPGDWDASFVIVQHHDPAHGHMLVEVLRKMAHMEVMEAADGTRIEPRHVYVIPPG
                     KNMSLLHGTLHLLPQSFEASRVLPIDFFFRSLAHDQGERSVGVILSGMGTDGTLGLRA
                     IKEAAGVAFVQAPSSAKFDGMPRSAIDAGLADVVLPVDELPRRIFSYRDHATAAHVST
                     ETRATTAQTAVEKICLLLRDYSGNDFTLYKRSTIYRRIERRMGLHQIHDHTQYLRFLR
                     EHPPEVEILFNELLIGVTSFFRDPGEWERLRHEILPGLLTQRASGGVLRAWVPGCSTG
                     EEAYSLAIVFREVLEPIKTLRNINVQIFATDIDRRAIEKARLGIYPENIAADVSPERL
                     RRFFVRDDRGYRVSKDIRETVVFAPQNVLTDPPFTKVDIVSCRNLLIYLSGEVQKRLI
                     PLFHYCLNPGGALFLGTAETIGSFTNLFVPLDGRTRLYRRLDQSQALPLDVQFASLVR
                     TRHLPEEPIVPQPPVKQASPNLQGVVDRLLVQRFAPLGILCDPHGEVLYVSGRAGKYL
                     EPAVGKTNVNIFAMAREGLRYELSRTFTKALRGGERPVTVRDLKVGTNGGTQRVNLAV
                     QKLAEPQELSGTVIVVISEAETGPAPSRRQPAPRGGPRTRELEDELQRSREQVQTIRE
                     EMQTSQEELKSTNEELQSTNEELQSTNEELTTSKEEMQSLNEELQTVNHELQSKVDEL
                     SRSNNDMKNLLNSTDIATLFLDADLRVRRFTTATAQIIKLIPADTGRPVTDIASDLDY
                     PQLADDAREVLRTLVFQEKQVVARDSRAFSVRIMPYRTLDNVIDGVVITFTDITAARA
                     LERAAREQADQLRQMAEALPHLVFGARADGAFDFISRQWVEYTGVPDVEQLQWLWLDQ
                     VHPADRERVRDEWRAAIRAGSHLDTEL"
BASE COUNT          460 a         1019 c          950 g          393 t
ORIGIN      
        1 gcgacggccg tcgcgcccgc gtcgccgccg ccgcccccgg aggacggcgc tgcgaccgcg
       61 catgccgcca gtccgttccc catcgtcggc atcggcgcat ccgccggcgg gctggaggcg
      121 ctggatcagt tcctgcgcaa catccccggc gactgggacg cgtcgttcgt gatcgtccag
      181 catcacgatc ccgcccacgg gcacatgctg gtcgaggtgc tgcgcaagat ggcgcacatg
      241 gaggtgatgg aggccgccga cggcacccgc atcgagccgc gccatgtcta cgtgatccct
      301 ccgggcaaga acatgtcgct gctgcacggg acgctgcacc tgttgccgca gtcgttcgag
      361 gccagccgcg tgctcccgat cgacttcttc ttccgctcgc tcgcccacga ccagggagag
      421 cgcagcgtcg gcgtgatcct gtccgggatg ggcaccgacg gcacgctcgg cctgcgcgcg
      481 atcaaggagg ccgccggcgt ggcgttcgtg caggcgccga gctcggcgaa gttcgacggc
      541 atgccgcgca gcgcgatcga cgccgggctg gccgacgtgg tgctgccggt cgacgagctg
      601 ccccgtcgga tcttctcgta ccgcgatcac gcgaccgcgg cgcacgtcag cacggagacg
      661 cgcgcgacca ccgcgcagac cgcggtcgag aagatctgcc tgctgctgcg cgactactcc
      721 ggcaacgact tcacgctcta caagcgcagc acgatctacc gccggatcga gcgccggatg
      781 gggctgcacc agatccacga ccacacccag tacctgcggt tcctgcgcga gcacccgccg
      841 gaggtcgaga tcctgttcaa cgagctcctg atcggggtca ccagcttctt ccgcgatccc
      901 ggcgagtggg agcggctgcg ccacgagatc ctgcccgggc tgctcacgca gcgcgcgtcc
      961 ggcggcgtgc tgcgggcctg ggtgccgggc tgctcgaccg gcgaggaggc gtactcgctg
     1021 gcgatcgtgt ttcgcgaggt gctcgagccg atcaagaccc tgcgcaacat caacgtgcag
     1081 atcttcgcga ccgacatcga tcgccgcgcg atcgagaagg cgcggctcgg gatctacccg
     1141 gagaacatcg cggccgacgt gtcgcccgag cggctgcgcc ggttcttcgt ccgcgacgac
     1201 cgcggctacc gggtcagcaa ggacatccgc gagaccgtcg tgttcgcgcc gcagaacgtg
     1261 ctgaccgatc caccgttcac caaggtcgac atcgtgtcgt gccggaacct cctgatctac
     1321 ctgtccggcg aggtccagaa gcggctgatc ccgctgttcc actactgcct caaccccggc
     1381 ggcgcgctgt tcctgggcac cgcggagacc atcggcagct tcaccaacct gttcgtgccg
     1441 ctcgacggtc ggacccggct gtaccgccgg ctcgaccagt cgcaggcgct cccgctcgac
     1501 gtccagttcg cgtcgctcgt ccgcacgcgc cacctgccgg aggagccgat cgtgccgcag
     1561 ccaccggtca agcaagcatc gccgaacctc cagggcgtgg tcgatcggct gctggtccag
     1621 cggttcgcgc cgctcgggat cctgtgcgac ccgcacggcg aggtgctgta cgtcagcggc
     1681 cgcgccggca agtacctgga gcccgccgtc ggcaagacga acgtcaacat cttcgccatg
     1741 gcgcgcgagg gcctgcgcta cgagctgtcg cgcacgttca ccaaggcgct gcgcggggga
     1801 gagcgtcccg tcaccgtgcg cgacctcaag gtcgggacca acggcggcac ccagcgcgtc
     1861 aacctggccg tgcagaagct ggccgagccc caggagctct ccggcaccgt gatcgtggtg
     1921 atcagcgagg ccgagaccgg gccggcgccg tcgcgacgcc agccggcgcc ccgcggcggc
     1981 ccgcgcacgc gcgagctgga ggacgagctg cagcgctcgc gcgagcaggt ccagaccatc
     2041 cgcgaggaga tgcagacctc gcaggaggag ctcaagtcga ccaacgagga gctgcagagc
     2101 accaacgagg agctgcagag caccaacgag gagctcacca cctccaagga ggagatgcag
     2161 tcgctgaacg aggagctgca gaccgtcaac cacgagctgc agtccaaggt cgacgagctg
     2221 tcgcggtcga acaacgacat gaagaacctg ctcaacagca cggacatcgc gacgctgttc
     2281 ctcgacgccg acctccgggt ccggcggttc accaccgcga ccgcgcagat catcaagctg
     2341 atccccgccg acaccggccg gccggtgacc gacatcgcgt ccgatctcga ctacccgcag
     2401 ctcgcggacg atgcgcgcga ggtcctgcgc acgctggtgt tccaggagaa gcaggtcgtg
     2461 gcgcgcgaca gccgcgcgtt ctcggtccgg atcatgccgt accgcacgct cgacaacgtg
     2521 atcgacggcg tggtgatcac gttcaccgac atcacggcag cccgcgcgct ggagagggcg
     2581 gcgcgcgagc aggccgacca gctgcgccag atggccgagg cgctgcccca cctggtgttc
     2641 ggcgcgcgcg ccgacggcgc gttcgacttc atcagccggc agtgggtcga gtacaccggc
     2701 gtgcccgacg tcgagcagct gcagtggctg tggctcgatc aggtccatcc cgcggaccgc
     2761 gagcgcgtgc gcgacgagtg gcgcgccgcg atccgcgccg ggagccacct cgacaccgag
     2821 ct
//
LOCUS       DASVOR010000765         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_358151,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000765 DASVOR010000000
VERSION     DASVOR010000765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358151"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            247..1131
                     /locus_tag="VF516_15120"
     CDS             247..1131
                     /locus_tag="VF516_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109062.1"
                     /translation="MASLAGRAAADEGAAGTGSGAATTDPGTDGGAVVKDDGFFTRIK
                     ILGSTTGQMQKDNATLSGGLGLSAENTRHFLYLLINSGRAEKPPASGAPSTSYGQFLL
                     NPPTTDLSLTLRDRYFLNDHALTLGAYAQLDGGRGDISATVAGAQETSQGFAVALDAG
                     AVGKMVISRQRNINLFLFTGLSSRFLGGDAARDDDLKLATLMTTKTAFFGANGSLLLQ
                     LGEVYAGLQLSYLSGNVPGLSGAQLIPVVGLRGGFDISTSAGKATAARPVGPAVVRAD
                     SPEAAQPPPAQPLPCVIF"
     gene            complement(1144..2382)
                     /locus_tag="VF516_15125"
     CDS             complement(1144..2382)
                     /locus_tag="VF516_15125"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8109063.1"
                     /translation="MLSRRTAWLVAIAATLTMTVSYIDRQALSVLSVAVMNDLDISKQ
                     QYGWLGSAFSFAYLFGTPLAGWWIDLAGARRGLLVSVLAWSAVAALHAVVPGFGVLFV
                     LRLALGITEGPGFPGAAQTIQRVLPAADRERGFGVLFTGSSFGAMVVPPFASWVYRQA
                     GWQVAFLVTTAAAGVLWLPLWYAVTRPQAVRARLDTAASAPSEPRPRFDEMVGHPLVL
                     RALAAIFCAAPVFAFPTFWGASFLNVTFGVTQGDVGHYLWLPPLVFDAAAITFGHLAA
                     RQRRPEGVPARGLVATGVALCAALALLPLTTTAWQATVGMSIAMAGSAVVYTLVTADL
                     LSRMPLGIASFAAGILAGSQSLALIIVSPLVGALVDRLHSYQVPTLALGLWAVPGGAV
                     WLAWRPALRFIPRARVHKAG"
     gene            complement(2417..>2908)
                     /locus_tag="VF516_15130"
     CDS             complement(2417..>2908)
                     /locus_tag="VF516_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109064.1"
                     /translation="YYSSEHDAMVMNIGPGAGTLVHEVVHPFVEANFPGAPSWFNEGL
                     ASLYERPTEKKGHIWGLPNWRLPNLQREIAARTLPAMTRMLGTTSEQFYNADFDAYAY
                     ARYLLLYLQDQGKLTEFYRAFVADRDDPTGRKALEAVLGEPLDSFEPRWRKWAVALRG
                     DNR"
BASE COUNT          460 a          998 c         1079 g          371 t
ORIGIN      
        1 tgatgatcga gtcggtgctc gtgcgggtcg cggtattcct catgacaagg atgtaatgcg
       61 cccggggccg gacgtgcccg atgtgcggcg gctgtgtgcc ggcgtcgccg tgaccagggc
      121 gctcgcctgc gcgagcagac cttcaccagc caaggtcgat cgatcgtgtc acgatcgtgc
      181 ggacaaagct gcactcaagg aaggagtggc gtgatgagct ctcgggcgta catgacgatc
      241 atcgtagtgg cgtcgctggc aggccgggcc gcggcggacg agggtgcggc tggcacgggc
      301 agcggcgcgg cgaccacgga ccccggcacc gacggcggcg cggtcgtcaa ggacgacggc
      361 ttcttcaccc ggatcaagat cctcggcagc acgacggggc agatgcagaa ggacaatgcc
      421 acgctgtcgg gcggtctggg gctgagcgcg gagaacaccc ggcacttcct ctacctgctg
      481 atcaacagcg gacgggccga gaagcctccc gcgagcgggg cgccgtcgac ctcgtacggc
      541 cagttcctgc tgaacccgcc gacgaccgat ctgagcctga cgctgcggga tcgctatttc
      601 ctcaatgacc atgctctgac gctcggtgcc tatgcccagc tcgacggcgg ccggggcgac
      661 atctccgcca cggtcgccgg cgcgcaggag accagccagg ggtttgccgt cgcgctcgac
      721 gcgggcgccg tcgggaagat ggtgatctcc cggcaacgca acatcaacct gttcctgttc
      781 accgggctgt cgtcgcggtt cctcggcggt gatgcggccc gcgacgacga tctgaagctg
      841 gccaccctga tgaccacgaa gaccgcgttc ttcggcgcca acggctcgct gctgctgcag
      901 ctcggcgagg tctatgccgg attgcagctg tcgtacctgt ccggcaacgt gccggggctg
      961 agcggcgcac agctgattcc ggtggtcggg ttgcgcggcg gattcgatat ctcgacctcc
     1021 gccggcaagg ccacggcggc ccgtccggtg ggcccggctg ttgtgcgcgc ggactcgccc
     1081 gaggcggcgc agccaccgcc ggcgcagccc ctgccgtgcg tcatcttctg atcgggcgga
     1141 ccctcagccg gccttgtgga cccgggcgcg cgggatgaac cggagcgccg ggcgccaggc
     1201 gagccagacc gcgccgcccg gcaccgccca caggccgagc gcgagggtcg ggacctggta
     1261 gctgtggagc cggtcgacga gggcgccgac cagcgggctg acgatgatca gcgcgagcga
     1321 ctgcgagccg gcgaggatgc cggcggcgaa cgacgcgatc ccgagcggca tgcgggagag
     1381 caggtcggcg gtgaccaggg tgtagaccac ggcggagccg gccatcgcga tcgacatgcc
     1441 gaccgtcgcc tgccacgccg tggtggtcag cgggagcagc gcgagggccg cgcacagcgc
     1501 cacgccggtg gcgaccaggc cgcgcgccgg gacgccctcg gggcggcgct ggcgggcggc
     1561 gaggtggccg aacgtgatcg ccgcggcgtc gaacaccagc ggcggcagcc acaggtaatg
     1621 gccgacatcg ccctgggtca cgccgaacgt cacgttgagg aagctggcgc cccagaaggt
     1681 cgggaacgcg aacaccggcg cggcgcagaa gatcgcggcg agcgcgcgca gcacgagcgg
     1741 gtgaccgacc atctcgtcga accgcggccg cggctcggag ggggcgctcg cggcggtgtc
     1801 gagccgggcg cgcaccgcct gcgggcgggt gaccgcgtac cacagcggga gccacagcac
     1861 gccggccgcg gcggtggtga ccaggaacgc gacctgccag ccggcctggc ggtacaccca
     1921 cgacgcgaac ggcgggacga ccatcgcgcc gaacgacgag ccggtgaaca gcacgccgaa
     1981 gccgcgctcg cggtcggccg ccggcaggac gcgctggatg gtctgcgcgg cgcccgggaa
     2041 cccggggccc tcggtgatgc cgagcgcgag ccggagcacg aacagcacgc cgaacccggg
     2101 caccacggcg tggagcgcgg cgaccgccga ccacgccagc accgagacca gcaggccgcg
     2161 gcgcgcgccg gcgaggtcga tccaccagcc ggcgagcggc gtgccgaaca ggtaggcgaa
     2221 cgagaacgcc gagccgagcc agccgtactg ctgcttggag atgtcgagat cgttcatcac
     2281 cgcgaccgac agcaccgaca gcgcctggcg gtcgatgtag ctcacggtca tcgtcaacgt
     2341 cgcggcgatc gcgacgagcc acgcggtgcg ccgggacagc atggcccgga cgatactcca
     2401 actcgcggcg cggggctcag cggttgtcgc cgcgcagcgc caccgcccac ttgcgccacc
     2461 tgggctcgaa gctgtcgagc ggctcgccga gcaccgcctc gagcgccttc cggccggtcg
     2521 gatcgtcccg gtcggcgacg aacgcccggt agaactcggt cagcttgccc tgatcctgga
     2581 ggtagagcag caggtagcgc gcgtaggcgt aggcgtcgaa gtcggcgttg tagaactgct
     2641 cgctggtggt gccgagcatc cgggtcatcg ccggcagcgt gcgcgcggcg atctcgcgct
     2701 gcaggttggg cagccgccag ttcggcaggc cccagatgtg ccccttcttc tcggtcggcc
     2761 gctcgtagag cgacgccagc ccctcgttga accacgacgg cgcgcccggg aagttggcct
     2821 cgacgaacgg gtgcacgacc tcgtggacca gcgtgccggc gccggggccg atgttcatca
     2881 ccatcgcgtc gtgctccgac gagtagta
//
LOCUS       DASVOR010000766         1940 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387164,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000766 DASVOR010000000
VERSION     DASVOR010000766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1940
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387164"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1227..1415)
                     /locus_tag="VF516_15135"
     CDS             complement(1227..1415)
                     /locus_tag="VF516_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109065.1"
                     /translation="MVRFELGPRFVPFSGTRAPGLPPGAASLAAPPEQLLLNVPLSVA
                     LPLRRHASFVTTIALTVN"
     gene            1724..>1940
                     /locus_tag="VF516_15140"
     CDS             1724..>1940
                     /locus_tag="VF516_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109066.1"
                     /translation="MTGDEPELRTARTSGATAEVAPFADDAALLQALFDAVTARAKAA
                     IAHRQWLGLLSRRGDAAATLFLDGDRID"
BASE COUNT          338 a          611 c          656 g          335 t
ORIGIN      
        1 cgacgggcac gccgccacgg taccaccgat gcacatcgct tcgcgcgccg cgatcgcatc
       61 ctggaggaac gccgtgacgg gcagatgcac ggtcaaagac acgatcggca gcgcgtcgtt
      121 cctgccaacc gcgccgcaga acacaaggga aggacctcgg gagaccgcac ggtggcttgt
      181 gaccggcccg agacagctcg ccaggcctac ttggacggcc cgctcacaac ccggggctcg
      241 cgccgatgct ccgttcggat ttcttctttt tttctcttcg gaggagaagc atcctcgggg
      301 agcaaggcgc cgcctgccgg atcgcgccgg caacagctcg acccgcgtcg tgtcgtggcg
      361 agcggccgtt gccagcgcgc gtcgacctgc tctgacgtct gcgctcagcg agccaccgcc
      421 tttcggcgtg cctccgtctg cgcggtatgc gccgccacct gcgcgttggc gaagcggagc
      481 gatgcgctcg cctgctccga cgctttcgtg gtgagtgact ggatccggcc gaacaccagg
      541 cgctggcggt cactcgtcgg gaacggaagc gcgtcggcgg cggctcgccg gtgtgaccgc
      601 accggcgggc cgctatgatc atgccttgcg cctatggcgg cgctgccttg ttccggagga
      661 acgtttctgt atcgccaggc cggcgtgaga agtcaatcca gtatcggtag cttgattcat
      721 ccattcgatg aacaatcata tttatagatt ggaacagtcg agatcttctg aatcagatcc
      781 ataaaaatca tcttccagcg agcgtatggt cgagggccag gactgtggag aacagaagga
      841 cggctgtgag ccgcgtatgg ggcgatctgg tcagctgcag cgcgggtcat caggcgtccc
      901 tgttctccat caggccgccc gaggttccag gggaccttcg ctcgaggatt cgtcgacgag
      961 gcacagctcg gcttcgccgt gcgttggcgc ggcatggggc cacggaacct gcccgcccag
     1021 ctacgctgcg gtgccccggt cccatgtgga tcagcccgtg gcacttgcgc gatcgcatcg
     1081 ccttggagtc gagatcggaa ttcggacctc atggaccctg gattcatgtc ttcgatcgta
     1141 tacatttgtc gatacgcgta catgattcca gttcgctcaa gccagaccgt aatcatcgaa
     1201 tatgaacgat aattagatat gcggattcaa ttgacagtca gcgcgatcgt ggtaacgaag
     1261 gaggcatggc ggcgaagcgg aagcgcgacc gaaagcggca cgttcagcag cagctgttcc
     1321 ggcggggcgg caagcgacgc ggcgccgggc ggaagcccag gggcgcgcgt gccggagaag
     1381 ggcacgaaac gaggcccaag ttcaaaccgt accatgtccc attctccagc ggcatctcgt
     1441 tccccgactg ggcggagctg gagggccagc catggatgtg gccgatccgc gagacatacg
     1501 atccgctggt ggttcgccgg ccgcaaacct ggttgctccg ggagggttgg actggtgcgg
     1561 atggtgaggc aggtagagcc tccccgtggt tcccttcgat cgaccatcgc gtacgagcga
     1621 tgcggaggcc gccggtctcg cgatgcccag cgctcacacg atcggggcgc aaaacccggc
     1681 gccggcggtg cgagcgtcgc gcagtttcgc atacgatgat gggatgaccg gggacgagcc
     1741 cgagttgcgc acggcgcgga ccagcggcgc caccgccgag gtcgcgccgt tcgcggacga
     1801 cgcggcgctg ctccaggcgc tgttcgacgc ggtcacggcg cgggcgaagg cagcgatcgc
     1861 ccaccggcag tggctgggcc tgttgtcccg gcgcggcgac gccgcggcca cgctgttcct
     1921 cgacggcgac cggatcgacg
//
LOCUS       DASVOR010000767         2337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_299862,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000767 DASVOR010000000
VERSION     DASVOR010000767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299862"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF516_15145"
     CDS             complement(<1..284)
                     /locus_tag="VF516_15145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109067.1"
                     /translation="MPRCAGCVDLQRGQTVEGSFFHEPVNTADYDAGSAIDHQILDNH
                     YDSMAQADAIATRVGEPNVRAAYFMGHVEAIGLGPSTRGEHSLAGLASWAP"
     gene            complement(259..672)
                     /locus_tag="VF516_15150"
     CDS             complement(259..672)
                     /locus_tag="VF516_15150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109068.1"
                     /translation="MYRRPAASPAILRYTTCMSTQRNVLVIGFDPHAFPEYDARPIVA
                     AIAVGMQRFEAEGIPAELCLVQPDATAEPEIVARLTARDWACVVIGGGIRKHDPVIEL
                     FERVIHLVRQHAPGAAIAFNTNPTDCLDAALRWLR"
     gene            682..1254
                     /locus_tag="VF516_15155"
     CDS             682..1254
                     /locus_tag="VF516_15155"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8109069.1"
                     /translation="MTMASRTAPHRYSYAEYLVYEQDSGMKHEYHGGEIIAMAGGSRR
                     HNALASRISAALEIGRKPGCVAFQSDQRVRILATGKATYPDASMVCGPIEGDPADPSG
                     ATITNPTLIIEVLSPTTEQNDRGDKWQHYQLLPSLQEYVLVSQSHPRVEYYRRLATGG
                     WEYHDATEGVVHLASGAVLDLARLYAQLPD"
     gene            complement(1262..1981)
                     /gene="deoD"
                     /locus_tag="VF516_15160"
     CDS             complement(1262..1981)
                     /gene="deoD"
                     /locus_tag="VF516_15160"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF004489.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0043096 - purine nucleobase salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX8109070.1"
                     /translation="MSVHLEARPGDIAPIVLLPGDPLRAQFVAERFLDGAVRHNQVRN
                     ALGYTGRYRRRPISVQSTGMGMPSFSIYAHELIESYAARVLIRIGTCGALQPELAIRD
                     VVLAMTASTDSAMNHHRFAGMDFAPAADFDLLRRAHELATGRGLAVRVGGVLTSDAFY
                     PDREDWWRIWARHGVLAAEMETAALYTLASRSGVRALSILTVSDHIVTGAHSSADERQ
                     TGFGAMAELALAVAASLPESG"
     gene            complement(2018..>2337)
                     /locus_tag="VF516_15165"
     CDS             complement(2018..>2337)
                     /locus_tag="VF516_15165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEX8109071.1"
                     /translation="AAAWANVLGRGDYVTPHTHAGAAWSAVLYVDAGDSDEARGGFLS
                     LRDPRAGAAMVITGANAHDSACTFHLYPRTGMLVVFPAWLLHWVPVYQGARPRISIAA
                     NLR"
BASE COUNT          346 a          801 c          867 g          323 t
ORIGIN      
        1 ggggcccagc tcgccagccc cgccagcgag tgctcgccgc gcgtcgacgg gccgaggccg
       61 atcgcctcga cgtggcccat gaagtacgcg gcacgcacgt tgggctcgcc gacccgcgtc
      121 gcgatcgcgt cggcctgcgc catcgagtcg tagtggttgt cgaggatctg gtgatcgatc
      181 gcgctgccgg cgtcgtagtc tgcggtgttg accggctcgt gaaagaacga gccctcgacg
      241 gtctggccgc gctggaggtc aacgcagcca gcgcagcgcg gcatcgaggc agtcggtcgg
      301 gttggtgttg aacgcgatcg cggcgccggg cgcgtgctgg cgcacgaggt ggatcacgcg
      361 ctcgaacagc tcgatcactg ggtcgtgctt gcggatgcca ccgccgatca cgacgcacgc
      421 ccagtccctg gcggtcaggc gggcgacgat ctcgggctct gccgtcgcgt ccggctggac
      481 caggcacagc tcggccggga tgccctcggc ctcgaaccgc tgcatgccga ccgcgatcgc
      541 ggcaacgatc ggcctggcat cgtactcggg gaatgcgtgg ggatcgaagc cgatcacgag
      601 gacgttccgc tgggtcgaca tgcaggtcgt gtagcgcagg atcgccggcg aggccgctgg
      661 tctgcggtac actggggtcg catgacgatg gcctcgcgaa cggcgcccca ccgctattcc
      721 tacgcggagt atctcgtgta cgagcaggac agcgggatga agcacgagta ccacggcggc
      781 gagatcatcg cgatggccgg agggtcccgg cgccacaacg cgctggcgtc acggatcagc
      841 gcggcgctgg agatcggccg caagcccggt tgcgtcgcgt tccagtcgga tcagcgggtc
      901 cggatcctgg ccaccggcaa ggccacgtac cccgatgcgt cgatggtgtg cggcccgatc
      961 gagggcgacc cggccgaccc gagcggagcg acgatcacga accccacgct gatcatcgag
     1021 gtgctgtcgc cgaccaccga gcagaacgac cgcggcgaca agtggcagca ctaccagctc
     1081 ctgccgtcgc tccaggagta cgtgctggtc agccagtcgc acccgcgcgt cgaatactat
     1141 cgccgtctgg cgaccggcgg ctgggagtac cacgatgcca ccgagggcgt cgtgcacctc
     1201 gcctccgggg ccgtgctcga tctggcgcgg ctctacgcgc agctgccgga ctgacctggg
     1261 actagcctga ctccggcagc gacgccgcga ccgcgagcgc gagctcggcc atggcgccga
     1321 agccggtctg gcgctcgtcg gcggacgagt gcgcgccggt cacgatgtga tcgctgacgg
     1381 tcaggatgct cagcgcgcgc accccggacc ggctcgcgag cgtgtagagc gccgcggtct
     1441 ccatctcggc ggcgagcacg ccgtgccgcg cccagatccg ccaccagtcc tcgcggtcgg
     1501 gatagaacgc gtcgctggtc agcacgccgc cgacgcggac cgcgaggccg cggccggtcg
     1561 cgagctcgtg ggcgcggcgc agcaggtcga agtcggcggc cggcgcgaag tccatgccgg
     1621 cgaaccggtg gtggttcatc gcggagtcgg tcgacgcggt catcgccagc acgacgtcgc
     1681 ggatcgcgag ctcgggctgc agcgcgccgc acgtaccgat ccggatcagc acgcgcgcgg
     1741 cgtagctctc gatcagctcg tgggcgtaga tcgagaacga cggcatgccc atgccggtgc
     1801 tctgcaccga gatcggccgg cggcggtagc gcccggtgta gccgagcgcg ttgcgcacct
     1861 ggttgtggcg gacggcgccg tcgaggaagc gctcggcgac gaactgcgcg cgcagcggat
     1921 cgccgggcaa cagcacgatc ggcgcgatgt cgccgggcct ggcttcgagg tgaacgctca
     1981 tgctcgctcc acgggcggcg catgatacac gcccggctca ccgcaggttg gccgcgatgc
     2041 tgatccgcgg ccgggcgccc tggtagaccg gcacccagtg cagcagccag gccgggaaca
     2101 cgacgagcat gccggtccgc gggtagaggt ggaacgtgca cgcgctgtcg tgggcgttgg
     2161 cgccggtgat caccatcgcg gcgccggcgc gcggatcgcg cagcgacagg aacccgccgc
     2221 gggcctcgtc gctgtcgccc gcgtcgacgt agagcacggc gctccacgcg gcgccggcgt
     2281 gggtgtgcgg cgtgacgtag tcgccgcggc cgagcacgtt ggcccacgcg gcggcgt
//
LOCUS       DASVOR010000768         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_65799,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000768 DASVOR010000000
VERSION     DASVOR010000768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65799"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1579)
                     /locus_tag="VF516_15170"
     CDS             complement(<1..1579)
                     /locus_tag="VF516_15170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109072.1"
                     /translation="MLFRVDADLLQLDDLVLRRGERIDEDARERLGLRKGRAKTLTNR
                     IHVAFCALAAARVYRSDGCLSVDEIGTLDGWSREGSVAKLVANEFEFGAGIIQQLGRG
                     GEAELRLRLPTAEIQLGELPETVQKLFDREQSASVPLDDLLPQPIVDRELATAVAEAL
                     RDASPDLTLLVRPAYQGGRSSLVFRGTALRTLGDALGQPPADAVLVKVPLGISGGKLA
                     VNARALDHERRLLTALAGWPGLPPRVETLRIDLRSLGEALRRQHHVHVHDEALAMDAL
                     VLPLGQLRTNEAEPRLLRSIIDETASPMRRLSRRDIHNALTLAERLAQLLRMLHRERR
                     AHRHLSVDSIVVRYELGIFGAVLAPDLAASVQVPRGGDDLLYRDLFELGRVLAHVITG
                     AALSRERQNFQPPEFPGRRLIKRAVVGEGETVARLLACVSRYLLEASPGADAERDDDR
                     PTASLLDHFLEEIEHIDMLHWARARGVPERRVSIAGATPAASQSAVPTREIWERSAAD
                     PVATRLVTISHLVDMATL"
     gene            1938..>2491
                     /locus_tag="VF516_15175"
     CDS             1938..>2491
                     /locus_tag="VF516_15175"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8109073.1"
                     /translation="MRRRALYLGANPPGTVSLGLDEEVRAIRQELRSARYRCFDLVPS
                     WGSQPNDLVRELRESSPAIVHLSGHACKAGGREVAPGSAHRDVRVDPATDVEGGGGLV
                     LHARDGSVQIVPYTVVKEIFELAGASVRLVVLTACNTEPLASLLLEHVDCVIGMDGPI
                     GDRAALEFSRELYATLGDGASVEQA"
BASE COUNT          381 a          817 c          931 g          362 t
ORIGIN      
        1 gcagggtggc catgtcgacg aggtgcgaga tcgtgaccag ccgggtcgcg accggatcgg
       61 cggcggatcg ctcccagatc tcgcgcgttg gcaccgcgct ctgggaggcg gccggcgtcg
      121 ccccggcgat cgagacccgg cgctccggca cgccgcgcgc gcgggcccag tgcagcatgt
      181 cgatgtgctc gatctcctcg aggaagtgat cgagcagcga cgccgtgggg cgatcgtcgt
      241 cgcgctcggc gtcggccccg ggcgaggcct cgagcaggta gcggctcacg caggccagca
      301 ggcgcgcgac ggtctcgccc tcgcccacca ccgcgcgctt gatcagccgg cggccgggaa
      361 actcgggcgg ctggaagttc tggcgctcgc ggctgagcgc ggctccggtg atgacgtggg
      421 cgagcacccg gcccagctcg aacaggtcgc gatacaggag gtcatccccg ccgcgcggga
      481 cctggaccga cgccgcgaga tcgggggcca ggaccgcccc gaagatcccg agctcgtagc
      541 gcacgacgat cgagtcgacc gacaggtgac ggtgcgcgcg gcgctcgcgg tggagcatgc
      601 gcaggagctg cgcgagccgc tcggcgaggg tcagcgcgtt gtggatgtcg cgccggctca
      661 gccggcgcat cgggctggcg gtctcgtcga tgatgctgcg cagcaggcgc ggctcggcct
      721 cgttggtccg cagctgaccg agcgggagca ccagcgcatc catcgccagc gcctcgtcgt
      781 ggacgtggac gtgatgctgg cggcgcagcg cctcgcccag cgagcgcaga tcgatgcgga
      841 gcgtctcgac ccgcggggga agtcccggcc agccggccag cgcggtgagc aaccggcgct
      901 cgtggtcgag cgcccgggcg ttcacggcga gcttgccgcc gctgatcccg agcggcacct
      961 tcaccagcac cgcgtcggcg gggggctggc ccagcgcgtc tcccagcgtt cgcagggccg
     1021 tgccgcggaa caccagggag ctccggccgc cctggtacgc cgggcggacc agcaaggtca
     1081 gatcggggct ggcgtcgcgc agcgcctcgg cgaccgcggt cgcgagctcg cgatccacga
     1141 tcggctgcgg cagcagatcg tcgagcggca ccgacgccga ctgctcgcgg tcgaacagct
     1201 tctggacggt ctcggggagc tcgccgagct ggatctcggc ggtcggcagg cggagccgga
     1261 gctcggcctc gccgccgcgg ccgagctgct ggatgatgcc ggcgccgaac tcgaactcgt
     1321 tggcgaccag cttcgccacc gagccctcgc gcgaccagcc gtcgagcgtt ccgatctcgt
     1381 cgaccgagag gcagccgtcg ctgcgataca cacgggccgc ggccagcgcg cagaacgcaa
     1441 catgaatacg attcgtgagg gttttcgctc ggcccttgcg aaggccgagg cgctcgcgcg
     1501 cgtcttcatc gatgcgctcg ccgcgacgga gcacgaggtc atcgagctgc agcagatcgg
     1561 catctacgcg aaataacatg cggagtacgg ggcttgctcg agtgctgcgg cggtgcggac
     1621 cgtggggtac ggacgaccct gcagcgaatt accgcacggt cgtgaatatc cattgtggac
     1681 gaaattgtgc tggatcatca agaggacttc tgacaggctc accgtacacg atgtcccgga
     1741 tgttcggaac gtatgtctcg cggatgaacc atcataaatt tcggtgaatt gaggtgggtt
     1801 atgctgtact ggcacacatg cagcgcggcg agtgcagggg aacgctcgtc acatgatgga
     1861 gagcattctg agcagattct gagatgtctc gagcgaagcg gcacgatgtc tcgccgtggc
     1921 gcacgcgggg gacggccatg aggcgcaggg cgctctacct cggggccaac ccgcccggga
     1981 cagtgagtct cgggctggac gaggaggtgc gcgcgatccg gcaggagctc cgcagcgccc
     2041 ggtaccgttg cttcgatctc gtgccctcct gggggagcca gccgaacgac ctggtccgcg
     2101 agctgcgcga gtccagcccg gcgatcgtcc acctcagcgg ccatgcgtgc aaggccgggg
     2161 ggcgggaggt ggcgccgggg agcgctcatc gcgacgtacg tgtcgatccc gcgacggatg
     2221 tcgagggcgg cggcgggctg gtactgcacg cccgcgacgg cagcgtgcag atcgtgccgt
     2281 acaccgtcgt gaaggagatc ttcgagctcg cgggagcgtc cgtgaggctc gtggtgctga
     2341 ccgcgtgcaa caccgagccg ctcgcgtcgc tgctgctcga gcacgtcgac tgcgtgatcg
     2401 gcatggacgg gccgatcggc gatcgcgcgg ccctggagtt ctccagggag ctgtacgcga
     2461 cgctcgggga tggcgcctcg gtcgagcagg c
//
LOCUS       DASVOR010000769         3102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000769 DASVOR010000000
VERSION     DASVOR010000769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1229
                     /locus_tag="VF516_15180"
     CDS             <1..1229
                     /locus_tag="VF516_15180"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX8109074.1"
                     /translation="QLASLTLGDGGAAVIVDRAPDGRPGRGEAGAVAGRQGGVMTAIA
                     AAPPWMLCETRPGPAAPFAARFESIGAYLPERRMTTRELMASTRHRTRIDLERLTGIR
                     EHRVCGPDETALSLAIAAARDCLARSRYTGADLDMVISASITRDADGATHRFEPPLSL
                     SIKRAIGAPAATSFDISNACAGMLTGVFVLNDFIRRGAIRRGMVVSGEHITGLGSNAA
                     QDIRSIFSLQLASLTLGDGGAAVIVDRAPDGRPGIVLAGFTTLAAHSRLCIGLPALHR
                     PGAKMYTRARKIHEVAMADGPILLEDVLGHAGAHLGEIDWLIPHQTSVRAIRAGERAL
                     AERLGEHPGHVGVTADEYGNTASTTLFLALHRYLTEGVLRAGEKILLLSVASGLQVGV
                     VLFVMDELEASHGHPH"
     gene            1213..2121
                     /locus_tag="VF516_15185"
     CDS             1213..2121
                     /locus_tag="VF516_15185"
                     /inference="COORDINATES: protein motif:HMM:NF020130.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEX8109075.1"
                     /translation="MGTRIEAAATAQPRSRWVGRGALHLSDTAAARCLRRAHQRPDDL
                     DLLINAGLYKDHNAAEPALAAIIQDDLGANRGHPPRVGHHGTFSFDVLNGGCGLITAA
                     QLIDAFVGHGGARFGLIVAADADPSPRTSRSFPFSPAGGAMLIAHTDDTAGFQRFALR
                     TFPEHAPLFEADLRWDPRAGATRRGRNVLEVHEAPGFGACCVDRAVEVATGFLADAGL
                     TAPDIDLVIASQYPRGFGLLVTRRLGIPTVRLPAVPPAFAAAHTAGPIAALETAITTG
                     RFARARHTLFVTAGAGLTIGLALYRR"
     gene            complement(2245..2700)
                     /locus_tag="VF516_15190"
     CDS             complement(2245..2700)
                     /locus_tag="VF516_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109076.1"
                     /translation="MINAPDMSTPVTRGELRAELAELETRLEQKLEQKLEQKLEQKLE
                     QLEQKLEQKLAPLATRAELEMWAGALMARMNDTDQRMTRLDQRMTGLEQHMIGMEQRL
                     VAELARHSRANAESMSAQITVIDEKYAGLPGRVKQLETDVSTLKRRRRG"
BASE COUNT          411 a         1207 c         1027 g          457 t
ORIGIN      
        1 tgcagctcgc gtcgctcacc ctgggcgacg gcggcgccgc ggtgatcgtc gatcgcgccc
       61 ccgacggccg cccgggccga ggcgaggccg gcgcggtcgc cggtcggcaa ggcggcgtca
      121 tgacggcgat cgcggccgcg ccaccgtgga tgctgtgcga gacccggccc ggcccggcgg
      181 caccgttcgc ggcgcggttc gagagcatcg gcgcgtacct gcccgagcgg cggatgacca
      241 cgcgcgagct gatggccagc acgcggcacc gcacccggat cgacctcgag cgcctgaccg
      301 ggatccgcga gcaccgcgtg tgcggccccg acgagaccgc gctcagcctc gcgatcgccg
      361 cggcgcgcga ctgcctggcg cgctcgcgct acaccggcgc ggacctcgac atggtgatca
      421 gcgcgagcat cacgcgcgat gccgacggcg cgacccaccg cttcgagccg ccgctcagcc
      481 tctcgatcaa gcgcgcgatc ggcgcgcccg ccgcgaccag cttcgacatc tcgaacgcgt
      541 gcgccggcat gctgaccggc gtgttcgtgc tcaacgactt catccggcgc ggcgcgatcc
      601 ggcgcggcat ggtggtgagc ggcgagcaca tcaccggtct cggcagcaac gccgcgcagg
      661 acatccgcag catcttcagc ctgcagctcg cgtcgctcac cctgggcgac ggcggcgccg
      721 cggtgatcgt cgatcgcgcc cccgacggcc gcccgggcat cgtgctggcg ggcttcacca
      781 cgctcgctgc ccacagccgg ctgtgcatcg ggctgccggc gctgcaccgg ccgggcgcca
      841 agatgtacac ccgcgcgcgc aagatccacg aggtcgcgat ggccgacggc ccgatcctcc
      901 tggaggacgt cctcggacac gccggtgcgc acctcggcga gatcgactgg ctgatcccgc
      961 accagacctc ggtccgcgcg atccgcgccg gcgagcgcgc gctcgccgag cggctcggcg
     1021 agcatcccgg ccacgtcggg gtcaccgccg acgagtacgg caacaccgcc tcgaccacgc
     1081 tgttcctcgc gctccaccgc tacctgaccg agggcgtgct ccgcgccggc gagaagatcc
     1141 tcttgctgtc ggtcgcctcc ggcctccagg tcggggtcgt gctgttcgtc atggacgagc
     1201 tggaggccag ccatgggcac ccgcattgaa gccgcggcca ccgcgcagcc gcgcagccgc
     1261 tgggtcggcc gcggcgcgct tcacctgagc gacaccgccg cggcccgctg cttgcgccgc
     1321 gcccaccagc gccccgacga tctcgacctc ctgatcaacg ccgggctgta caaggatcac
     1381 aacgctgccg agccggcgct cgccgcgatc atccaggacg acctcggcgc caaccgcggc
     1441 cacccgccgc gggtcggcca ccacggcacg ttctcgttcg acgtgctcaa cggcggctgc
     1501 ggcctgatca ccgccgcgca gctgatcgat gcgttcgtcg gccacggcgg cgcccggttc
     1561 ggcctgatcg tcgcggccga cgccgacccg tcgccgcgca cctcgcgcag cttcccgttc
     1621 tcgcccgccg gtggcgcgat gctgatcgcg cacaccgacg acaccgccgg cttccagcgc
     1681 ttcgcgctgc gcacgttccc cgagcacgcg ccgctgttcg aggccgatct gcgctgggat
     1741 ccgcgcgccg gcgccacccg ccgcggtcgc aacgtgctcg aggtccacga ggccccgggg
     1801 ttcggggcgt gctgcgtcga ccgcgccgtc gaggttgcca ccggcttcct cgccgacgcc
     1861 ggcctgaccg cccccgacat cgacctcgtg atcgccagcc agtacccgcg cggcttcggc
     1921 ctcctggtca cccgccggct cggcatcccg accgtccggc tgcccgcggt cccgcccgcg
     1981 ttcgccgccg cccacaccgc cggcccgatc gccgccctcg agaccgcgat caccaccggc
     2041 cggttcgccc gtgcccgcca tacgctgttc gtcaccgccg gcgcgggcct caccatcggc
     2101 ctggcgctct accgccgctg accgtcggct cgcggcgcgg ccggccctcc gggtgaccgt
     2161 accgacctcg actgcctcac gcggatcggg ctacgcctcc agctcgccac gtgccactgg
     2221 cgtcgacatg gagcggatcc ctcgtcatcc tcgtcggcgc cgcttgaggg tggacacatc
     2281 ggtctcgagc tgcttcacgc ggcccggtag gccggcgtac ttttcatcga tcaccgtgat
     2341 ctgcgcggac atcgactcgg cgttcgcccg gctatgacgc gcgagctctg caaccaggcg
     2401 ttgctccatg ccaatcatgt gttgctccag gccagtcatg cgttggtcca agcgagtcat
     2461 gcgttggtcg gtgtcgttca tacgagccat cagggctcct gcccacatct ccagctccgc
     2521 cctggtcgcc agaggcgcga gcttctgctc gagcttctgt tcgagctgtt cgagcttctg
     2581 ttcgagcttc tgttcgagct tctgctcgag cttctgctcg agccgtgtct cgagctctgc
     2641 gagctcggcc cgcaactcgc cgcgtgtcac cggggtcgac atatcaggtg cgttgatcat
     2701 caccctacct cctggcacgc gggagcgaga agcagctccc gcggtgatcg cacagcgccg
     2761 cgaagcgggg cgtgcatgcg ccgaccggag gagcgcttgc ttcgaccgga gccggcgtcc
     2821 tgcgcgtcgc ccagtgggca tggagaaagg gatcgaacat catcttggaa acaggtcatt
     2881 tccgtcggag cagcaacttg cttgccgacg tgcgcccgcg tgatgctgca cagttggcgg
     2941 atcgcgtggc cgatatccgg ccagggcgtc cgaaatccgg ccggacaccg gacacggaca
     3001 cgaacaaggc gacgcggccc gcgatcgatg cttcacgccg agggcagttg cgcaggtgga
     3061 tggcgggccg cccgcgctgc gcagcggacc gcgcaccgca cc
//
LOCUS       DASVOR010000770         1830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_563118,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000770 DASVOR010000000
VERSION     DASVOR010000770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1830
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563118"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..739)
                     /locus_tag="VF516_15195"
     CDS             complement(<1..739)
                     /locus_tag="VF516_15195"
                     /inference="COORDINATES: protein motif:HMM:NF017396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070008 - serine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S28 family serine protease"
                     /protein_id="HEX8109077.1"
                     /translation="MNRAVGFIVAALVAGSGCGDDSGPPPMTNDELMARLRALHGVTV
                     QEGDSAPENFSYYILHFTQPIDHHDPSKGTFQQQVSLLHHNELAPFPLIVYTSGYADE
                     DGGTPVELTTLLDANQISIEHRFYGTSQPDPVDWSMLTVEQAAMDEHDIIQALRTIYA
                     GSFLSVGESKGGITALAHRRFYPDDVRGTVAYVAPMSFNVPDPRYPDQLDQVGMPTLS
                     DCHAAVRDLASMMLSRRAQMLDLARSDA"
     gene            857..>1830
                     /gene="ychF"
                     /locus_tag="VF516_15200"
     CDS             857..>1830
                     /gene="ychF"
                     /locus_tag="VF516_15200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEX8109078.1"
                     /translation="MALSVGIVGLPNVGKSTVFNALTSGKADAANYPFCTIDPNVGIV
                     PVPDDRLRRIESHIKTQKVIPAIVEIVDIAGLVAGASQGEGLGNKFLANIRETNAILM
                     MVRCFEDPNVIHVAGSVDPMRDIEVIDLELALADVETAEKRLKKAQGAAKTGSKEGKA
                     ELAFTERVYAHLQTGKAARTLALSEDERKQIATWGLLTAKPVLYCCNVGEADLPRGNA
                     WSDAVRARAQHEGAGVVILCGKVEAELAELPPGDRAELLASYGLAEPALATLARECYR
                     LLGLQSYFTAGEKEIRAWTIRKGALAPEAAGVIHTDFEKGFIRAQVYSV"
BASE COUNT          319 a          599 c          623 g          289 t
ORIGIN      
        1 cggcgtcgga cctggcgaga tccagcatct gcgcgcggcg gctcagcatc atgctggcga
       61 ggtcgcgcac ggcggcgtgg caatcggaca gcgtcggcat gccgacctga tcgagctgat
      121 ccggatagcg cggatccggc acgttgaacg acatcggcgc gacgtacgca acggtgccgc
      181 ggacgtcgtc gggatagaac ctgcggtggg ccagcgcggt gatcccgccc ttggattcgc
      241 cgacgctgag gaacgagcca gcgtagatcg tgcgcagcgc ctggatgatg tcgtgctcgt
      301 ccatcgccgc ctgctcgacc gtgagcatgc tccagtcgac cggatccggc tgcgacgtgc
      361 cgtagaagcg gtgctcgatc gagatctggt tggcgtcgag cagcgtggtc agctcgaccg
      421 gggtgccgcc gtcttcgtcc gcgtagccag aggtgtaaac gatcagtggg aacggagcga
      481 gctcgttgtg gtgaagcagg gagacctgct gctggaacgt ccccttcgag gggtcgtggt
      541 ggtcgatcgg ctgcgtgaag tggagaatgt agtacgagaa attctcgggg gccgagtcac
      601 cctcctgcac cgtcacaccg tgcagagcgc gcagacgagc catcagctcg tcgttggtca
      661 tggggggcgg tccggagtcg tctccacatc cgctgccggc caccagcgcg gcaacgatga
      721 atccaaccgc ccgattcatg gggccaaact ctgccataga attccccggc cccgacccta
      781 gcaagcgcca actttttgat ggggatgcac cgctaccttg cgatcccagc gagatccagg
      841 gtaggcattg cccgccatgg cgctgtccgt cgggatcgtg ggcctgccga acgtcggcaa
      901 gagcactgtg ttcaatgctc tcacctcggg aaaagccgac gcggctaact atccgttctg
      961 caccatcgat cccaatgtag ggatcgtacc ggtgcccgat gaccgcctgc ggcgcatcga
     1021 gagccacatc aagacgcaga aggtcatccc cgcgatcgtc gagattgtcg acatcgccgg
     1081 cctggtcgca ggagcgtcgc agggcgaggg cctcggcaac aagttccttg ccaacatccg
     1141 tgagaccaac gccatcttga tgatggttcg gtgcttcgag gacccgaacg tgatccacgt
     1201 cgcggggtcc gtcgacccga tgcgcgacat cgaggtgatc gatctggagc tcgcgctcgc
     1261 cgatgtcgag accgccgaga agcggctgaa gaaggcccag ggcgcggcga agaccgggag
     1321 caaggagggc aaggccgagc tcgcgttcac cgagcgcgtc tacgcccacc tccagaccgg
     1381 caaggcggcg cgcacgctcg cgctgtccga ggacgagcgc aagcagatcg cgacctgggg
     1441 cctgctcacc gccaagccgg tgctgtactg ctgcaacgtc ggcgaggccg atctaccgcg
     1501 cggcaacgcg tggagcgacg cggtgcgcgc gcgcgcccag cacgagggtg ccggcgtcgt
     1561 gatcctgtgc ggcaaggtcg aggccgagct cgccgagctg ccgcccggcg accgcgccga
     1621 gctgctcgcg tcgtacggcc tcgccgagcc cgcgctcgcg acgctcgccc gcgagtgcta
     1681 ccgcctgctc ggcctgcagt cgtacttcac cgccggcgag aaggagatcc gcgcctggac
     1741 gatccgcaag ggcgcgctcg ctcccgaggc ggccggcgtg atccacaccg acttcgagaa
     1801 gggcttcatc cgcgcccagg tctactcggt
//
LOCUS       DASVOR010000771         3141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_475221,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000771 DASVOR010000000
VERSION     DASVOR010000771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475221"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF516_15205"
     CDS             <1..346
                     /locus_tag="VF516_15205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109079.1"
                     /translation="LAGGSAAPARPAPAAPVAPAPRGPDGYAPGPPSARPSPRQQAVV
                     VDDPSLSLPIAARRPWALIVVVLLIDLGLAAAGIWMLTQGLSERSSRPAPARSGERTV
                     PAGAAPSPGRPS"
     gene            343..1401
                     /locus_tag="VF516_15210"
     CDS             343..1401
                     /locus_tag="VF516_15210"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8109080.1"
                     /translation="MTRSETSSSYRSPSTGAHHLVGIEVLVLDGDRSVHAGIEQLLSE
                     AHLHVTCVTDAERAQALVERQFFSVALVDIDTPAPRAGIATIRAIKQASPTSMVIALT
                     PRRSFDDAVDAVRAGAIDLVLKAPESVAYLQERVIDAAGRSVGKREVDSVLDDVRGVH
                     EEFLQRFMDAERRAIDLADKLAGRDRSAAERVEELRVLVVDEVDDFVTAMTDAAPPGF
                     AFVHATSGGEGLDRISSGRFHYAMIAEDVSDLPARTIARTVRNQHPDTVVLTFLGPAD
                     NGRVDLVETHGSRPLLQPFRDAQQLVARLDELAEAWRAKARERRYTQAFRERHYDFLR
                     RYVELKTKIERALHDGPG"
     gene            complement(1408..2529)
                     /locus_tag="VF516_15215"
     CDS             complement(1408..2529)
                     /locus_tag="VF516_15215"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX8109081.1"
                     /translation="MKVNPDVFFKFHDGKFVVWNYRAHEQFELTLPYLQRLYAIGTAE
                     SQPVALSTASPVASLATPPAAPEPAQIDAELRAADLVSASYPGAAWGWDSLSHIFHVG
                     TSSLLAPGAALPREDSAEAYLESCKSMDPAEPAVIPALDGEVFALPTPDVERLERLSL
                     WDALIARKTAREFTGEPVDLEAVSNILWATFGAVHGPDRGDVDVFGVKSYGYRRTSPS
                     GGSLQTTEPYLVNLTITGLPAGVYHYHSIGHRLTRIDGEIPAADLGPLLAGQTFANDL
                     GFAIFMVARFDKMWWKYPHSRSYRVALMDVGHLSQTFHLVCTGYGLTSWLTGAFYDEE
                     IARRLRLDPARQAVLLMVGAGKGAGRAISPAILKALSSP"
BASE COUNT          472 a         1141 c         1063 g          465 t
ORIGIN      
        1 gctcgccggc gggtcggcgg ctccggcgcg cccggccccc gcggccccgg tcgcgccggc
       61 cccgcggggg cccgacgggt acgcgcccgg ccctccgagc gcgcggccat cgccgcggca
      121 gcaggccgtg gtggtcgacg atccatcctt gtcgctgccg atcgcggcgc gccggccgtg
      181 ggcgctgatc gtcgtcgtgc tgctgatcga cctcggcctc gccgccgccg gcatctggat
      241 gctgacccag ggcctgagcg agcggtcgag ccgccctgcg ccggcgcgat ccggagaacg
      301 caccgtgccg gcgggcgccg caccctcccc tggccggccg tcatgacccg ctccgagacc
      361 tcgagctcgt accgctcccc gtcgaccggc gcgcaccacc tggtcgggat cgaggtcctc
      421 gtgctcgacg gcgatcgcag cgtccacgcc ggcatcgagc agctgctgtc ggaggcccac
      481 ctgcatgtca cctgcgtgac cgacgccgag cgcgcgcagg cgctggtcga gcgccagttc
      541 ttctcggtcg cgctggtcga catcgacacg ccggcgccgc gcgccgggat cgcgacgatc
      601 cgcgcgatca agcaggcatc gccgacctcg atggtgatcg cgctgacccc gcggcggtcg
      661 ttcgacgacg cggtcgacgc ggtgcgggcc ggcgcgatcg acctggtgct caaggcgccc
      721 gagtcggtcg cctacctcca ggagcgcgtg atcgacgccg ccgggcgttc ggtcggcaag
      781 cgcgaggtcg actcggtgct cgacgacgtg cgcggcgtcc acgaggagtt cctgcagcgc
      841 ttcatggacg ccgagcgccg cgcgatcgat ctcgccgaca agctcgccgg ccgcgatcgc
      901 tcggccgccg agcgggtcga ggagctgcgc gtgctggtgg tcgacgaggt cgacgacttc
      961 gtgaccgcga tgaccgacgc cgcgccgccg ggcttcgcgt tcgtgcacgc gacctcgggc
     1021 ggcgagggcc tcgaccggat cagctccggc cgcttccact acgcgatgat cgccgaggac
     1081 gtgtccgacc tgccggcgcg gacgatcgcc cgcacggtcc gcaaccagca ccccgacacc
     1141 gtggtgctga cgttcctcgg cccggccgac aacgggcggg tcgatctggt cgagacccac
     1201 ggctcgcgcc cgctgctcca gccgttccgc gacgcccagc agctggtcgc ccggctcgac
     1261 gagctggccg aggcctggcg ggccaaggcg cgcgagcgcc gctacaccca ggcgttccgc
     1321 gagcgccact acgacttcct gcgccgctac gtcgagctca agaccaagat cgagcgcgcg
     1381 ctgcacgacg gccccgggta gccggcgcta cggcgagctc agcgccttca ggatcgccgg
     1441 gctgatcgcc ctgccggcgc ccttgccggc accgaccatc agcagcaccg cctggcgcgc
     1501 cggatcgagc cgcagccgcc gggcgatctc ctcgtcgtag aacgccccgg tcagccagga
     1561 cgtcaatccg tatccggtgc acacgaggtg gaaggtctgc gacaggtgcc cgacgtccat
     1621 cagcgccact cgatacgatc gcgagtgcgg atacttccac cacatcttgt cgaaccgcgc
     1681 gaccatgaag atcgcgaacc ccagatcatt ggcaaaggtc tgaccggcga gcagcggccc
     1741 gaggtccgcg gccgggatct cgccatcgat cctcgtcaat ctgtgaccaa tagaatggta
     1801 atgatacaca ccggccggta gcccggtgat cgtcaggttg accaggtagg gttccgtggt
     1861 ctgcaggctg ccgcccgacg gcgaggtccg gcgatagccg tagctcttga ccccgaacac
     1921 gtcgacatcg ccgcggtccg ggccgtggac cgcgccgaac gtggcccaca ggatgttgct
     1981 gaccgcctcg agatcgaccg gctcgccggt gaactcgcgc gcggtcttcc gggcgatcag
     2041 cgcgtcccac agcgacagcc gctcgagccg ctcgacgtcg ggggttggca gcgcgaacac
     2101 ctcgccatcc agggccggga tcacggccgg ctcggcggga tccatcgact tgcaggactc
     2161 gaggtaggcc tcggcgctgt cttcccgggg cagcgccgcg ccgggggcga gcagcgagct
     2221 ggtcccgacg tggaagatgt gcgagaggct gtcccagccc cacgccgcgc cggggtagct
     2281 cgccgacacc agatcggccg ctcgcagctc cgcatcgatc tgcgccggct ccggcgcggc
     2341 cgggggcgtg gccagggacg cgaccgggga cgcggtagac agtgcgacgg gctgcgactc
     2401 cgcagtgccg atcgcgtaca gccgctgcag gtacggcagc gtcagctcga actgctcgtg
     2461 tgcccggtag ttccagacaa cgaacttgcc atcatgaaac ttgaagaaga cgtccgggtt
     2521 gactttcatt cgaaacaaac tccgaaatca ccattccatt tccccgcgac caggtggtgg
     2581 agcaatgcgc ggcgctggac aaacgcacag cggcggcccg cgctgcggcg tgccacgccg
     2641 gcaccggcgc gcccgtctcg gcgccgccgc cgcgaatgca gctcccgtac aacggagctc
     2701 gcggcgctac ttcttcttga cgatcaacag ccactgcagc ggtatccaat tcgtacactc
     2761 gaggtttatt ttcttcattc gattctttct ccctggttgg caggtgggag tcgtgattct
     2821 gtacgctctc ggcaaactcc gcaaggccgc acgacgcgag acacactagt tgacaacggc
     2881 gcacgcgctg tctggtcttg gcaatggaat gatctgcacg tgcagctttg ttagccacag
     2941 atcatcgctg tcattccggc gagatatcgg ctcactcgga ccaccgtcgg accggcgccc
     3001 gggctcggcg gccagcgcgc cggagcgcgg ctcgatcgca gcttcgatcg cagccgggat
     3061 cgcaacctcg atcgcgtctc catgcgcggc ggagcgcgac gaacggtcgc agccgatacg
     3121 cgcgcgcggc agtgctgtgc c
//
LOCUS       DASVOR010000772         3074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_65867,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000772 DASVOR010000000
VERSION     DASVOR010000772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3074
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65867"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF516_15220"
     CDS             complement(<1..272)
                     /locus_tag="VF516_15220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109082.1"
                     /translation="MVKVGDPSYASELFVLQFLAEPGMQLGVVTTSYPRFPGDPAGNF
                     VAAHVAALRALGHHVEVIAAGGGATDNEPRDAARGGVNRPALPGEPP"
     gene            complement(603..1391)
                     /locus_tag="VF516_15225"
     CDS             complement(603..1391)
                     /locus_tag="VF516_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8109083.1"
                     /translation="MYRELRIAVVIPAFNERGKIAATVATVPELVDHILVIDDASRDD
                     TSRQAELAALRRAEPARVEVIRHAANRGVGGAIITGYRRAIAIGAEVAAVMAGDGQMD
                     PHDLPALLDPIADGTADYVKGNRFLHPAIWTAMPATRILGNAALSMATRVTSGYRHVF
                     DSQCGYTAIHRRALAAIDLDKLFPRYGYPNDLLSRLRVADMRVVDVPVRPIYGPAWRS
                     GIHLGTVLHPMPWVLLRSFATRLAAQARRRLLPSEPVPLAPEQA"
     gene            1510..1989
                     /locus_tag="VF516_15230"
     CDS             1510..1989
                     /locus_tag="VF516_15230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEX8109084.1"
                     /translation="MAGGVNKVILVGHLGADPDMRYTPRGQGVCELRLATSESWNDKN
                     GQRQERTEWHRIVVWGKRAEVCSKYLSKGRQVFVEGRIQTRTYDDKDGNKRYITEIIA
                     NDVQFLGGGRDGGGGGGGRGGRSDEGPPPPPSDGDFGGYGGGGGGGGGGGPDDDIPF"
     gene            2116..2484
                     /locus_tag="VF516_15235"
     CDS             2116..2484
                     /locus_tag="VF516_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109085.1"
                     /translation="MRIALTSLAIAAAIVPGAAGAAPASQAERPRGLSLEQIRGVMRA
                     HASEFNACFVAARRHDRQLHGPFVYQIEVDKKGKVTLARPYQPSGVATFDRCVIDVLL
                     RARFPGGPASVETPLVFEPS"
     gene            2499..>3074
                     /locus_tag="VF516_15240"
     CDS             2499..>3074
                     /locus_tag="VF516_15240"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8109086.1"
                     /translation="MTPATTPSPDADRPPRAERDLWGHADFLRLWAAQAVSAFGSRIT
                     RTALPIIAVSTLHKPAAMVGVLGAMQLIPGIPLAMVAGGVVDRGHKRRILIAADVIRA
                     ALVASLTVAWALDALVMSHVILVGAGVGAATALFQITDLAYLPQLLGRRRLAEGNAKL
                     ETTEAIAEITGPASAGVLIGALGAPLAVAIDA"
BASE COUNT          449 a         1066 c         1132 g          427 t
ORIGIN      
        1 ggcggctcgc ccggcaaagc cgggcgattc acccctccgc gcgccgcgtc gcgcggctcg
       61 ttgtcggtgg cgccgccgcc ggccgcgatc acctcgacgt ggtggccgag cgcgcgcagc
      121 gcggcgacgt gggccgcgac gaagttgccg gcgggatcgc cggggaaccg ggggtacgac
      181 gtggtgacga cgccgagctg catgccgggc tcagccagga actgtagaac aaagagctcg
      241 gacgcatacg acggatcgcc gaccttgacc atgacctcga cgtcgacccc cgcgttgatc
      301 ttgacggcag cgaagcgaca cgtaaacgtc tgaccccacc ttcgacctcg acctcgcccg
      361 gtcatcgcgc tccgtcgagg ctcgcggcac aggatcggtc gacgtccaac gtcgatggtg
      421 gactcgacgt cgtacgtcgc cgtcaatctc aacgctccgg gtcaacgatc aggtcgacgt
      481 cgacgatcac gaacgcccta tcgaagactc gtgacggatc atcagacgtc gagatgtacc
      541 tcgacggtcc ggatcgtgcg ccgagctcgt ccgacggcct tgacctacac agcttcgttc
      601 cttcaggcct gctccggggc gagcggcacc ggctcgctgg gcaacaggcg gcggcgggcc
      661 tgggcggcga gccgggtcgc gaacgaccgc agcagcaccc agggcatcgg gtgcagcacg
      721 gtgcccaggt ggatgccgct gcgccacgcc gggccgtaga tcggccggac cggcacgtcg
      781 acgacgcgca tgtcggcgac gcgcagccgc gacagcaggt cgttggggta gccgtagcgc
      841 gggaacagct tgtcgaggtc gatcgcggcg agcgcgcggc ggtggatcgc ggtgtaaccg
      901 cactgcgagt cgaacacgtg gcggtagccc gaggtcaccc gggtcgccat cgacagcgcg
      961 gcgttgccga ggatgcgggt cgccggcatc gcggtccaga tcgccgggtg gaggaagcgg
     1021 ttgcccttga cgtagtcggc ggtgccgtcg gcgatcgggt cgagcagcgc cggcaggtca
     1081 tgcgggtcca tctgaccgtc gcccgccatc accgccgcga cctcggcgcc gatcgcgatc
     1141 gcgcggcggt agccggtgat gatcgcgccg ccgaccccgc ggttggcggc gtggcggatg
     1201 acctcgacgc gggccggctc ggcgcgccgc agcgcggcca gctcggcctg gcgcgaggtg
     1261 tcgtcgcgcg acgcgtcgtc gatcaccagg atgtggtcga ccagctcggg gacggtcgcc
     1321 acggtggcgg cgatcttgcc gcgctcgttg aaggccggga tcacgacggc gatgcgcagc
     1381 tcgcggtaca ccccggtggt tgtaaatcgc cccggccggg gcgtccaccg tcgcagttgt
     1441 catagccgcg tgctatctgc tccccatcga cgggatggag tcatcccgga acgcgaaaga
     1501 aggaccatca tggccggcgg agtcaacaaa gtcatcctcg taggacacct cggtgccgat
     1561 cctgacatgc gctacacacc gaggggtcag ggcgtgtgcg agctccgcct cgccaccagc
     1621 gagagctgga acgacaagaa cgggcagcgc caggagcgca ccgagtggca ccggatcgtg
     1681 gtgtggggca agcgcgccga ggtctgctcg aagtacctgt cgaagggccg ccaggtgttc
     1741 gtcgagggcc ggatccagac ccggacctac gacgacaagg acggcaacaa gcgctacatc
     1801 accgagatca tcgccaacga cgtccagttc ctcggcggcg gccgcgacgg cggcggcggt
     1861 ggcggcgggc gtggcggccg gtccgacgaa ggtccgcctc ctccgccgtc ggacggcgac
     1921 ttcggcggct acggcggggg cggaggcggt ggcgggggcg gtggccccga cgacgacatc
     1981 ccgttctgac cggcgttcga tcggagccgg caacgccggg ggtcgccgca cggttgccag
     2041 ggcgcggccg gtctcggaca tctcgttcgg aggtccgttg ccagggcgcg cggagtctcg
     2101 cataacgtgg gttcgatgcg gatcgcgctg accagcctgg cgatcgcggc ggcgatcgtg
     2161 cccggggccg ccggcgccgc gccagcctcg caggccgagc ggcccagggg cctgagcctc
     2221 gagcagatcc gcggcgtgat gcgcgcccac gcgtccgagt tcaacgcatg cttcgtcgcc
     2281 gcgcgccgcc acgaccggca gctccacggg ccgttcgtct accagatcga ggtcgacaag
     2341 aagggcaagg tgaccctggc gcgtccgtac cagccgtccg gcgtcgcgac cttcgatcgc
     2401 tgcgtcatcg atgtactgct gcgcgcccgg ttcccgggtg ggccggcgag cgtcgagacg
     2461 ccgctcgtct tcgagccgag ctgatcgccg atccgaacat gacccctgcc acgacgccgt
     2521 cgccggacgc tgaccgcccc ccacgggccg agcgcgacct gtggggccac gccgacttcc
     2581 tccggctgtg ggcggcccag gcggtcagcg cgttcggctc gcggatcacg cgcaccgcgc
     2641 tgccgatcat cgcggtgagc actctccaca agcccgcggc gatggtcggc gtgctcggcg
     2701 cgatgcagct gatccccggg atcccgctgg cgatggtggc gggcggggtg gtcgaccgcg
     2761 gccacaagcg gcggatcctg atcgcggccg acgtgatccg cgcggcgctg gtcgcgtcgc
     2821 tgacggtcgc gtgggcgctc gacgcgctgg tgatgtccca cgtgatcctg gtcggcgccg
     2881 gggtcggcgc ggcgaccgcg ctgttccaga tcaccgacct ggcgtacctg ccgcagttgc
     2941 ttggccggcg gcggctcgcc gagggcaacg ccaagctgga gaccaccgag gcgatcgccg
     3001 agatcaccgg cccggcgagc gccggcgtgc tgatcggcgc cctcggcgcg ccgctcgcgg
     3061 tcgcgatcga cgcc
//
LOCUS       DASVOR010000773         7935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_95671,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000773 DASVOR010000000
VERSION     DASVOR010000773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7935
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95671"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VF516_15245"
     CDS             <1..699
                     /locus_tag="VF516_15245"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8109087.1"
                     /translation="DPASTEILTSLVEQSAAPLCAIENGQYAIDSTGFSTVTYDRWFD
                     QKHGKLNARHQWVKLHIACGTVTHAITAARVTSEGDCPQLPALLASTMKHHDVRELSA
                     DKAYSSVDNHNVMESLNVEAFVPFKVNAVVNPKAPVWSRHLVEFQLNQERFLAHYHRR
                     SAVETTFAMIKARFGASVASRLPTARANEVLAMCVAHNACCLVKAIFTAGLAPAFWQD
                     APAALLSLVPEEQA"
     gene            696..992
                     /locus_tag="VF516_15250"
     CDS             696..992
                     /locus_tag="VF516_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109088.1"
                     /translation="MSDLTAKEQKAVRTALRFLRLRAGAWKPLVKALRYEYDSIQKVA
                     SGKKAVTPALALRVARFVGIPMDELLAGQWLSPRTCPHCGHPPDDFVDEDTMPG"
     gene            1024..1449
                     /locus_tag="VF516_15255"
     CDS             1024..1449
                     /locus_tag="VF516_15255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109089.1"
                     /translation="MVRTDYVLAVMAAAQVPLSPVEVQKLFFILDKKAGAQLGGPFFN
                     FTPYAYGPFDAAVYHELERLAERGLASIYGQSTTCRYGISAAGLKPGAAALGAITMSG
                     YISQVAEYVRSSSFAQLILAVYAEWPEMMANSIFQGYPR"
     gene            1446..2219
                     /locus_tag="VF516_15260"
     CDS             1446..2219
                     /locus_tag="VF516_15260"
                     /inference="COORDINATES: protein motif:HMM:NF012452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109090.1"
                     /translation="MTLLVGIRCSDGVVVAADRAVTLGSMEQPTISHPACKISVLDGS
                     VIVAGSGYTGTLQRFCDVVEDTLLNCADCAGYQQKRPIEIGRILCTAGRKDFQSTMMK
                     DGIFGALVAFPSVMGRGPQLCEFHEKDFQPDLKDAGTWFVSMGYGRPIADSYLAFMRD
                     TFWRGGMPTLAHGIFSAAWVMQQSIRVSPGFIDEPIDIAVLQGEKARMLEPRELTYHM
                     GAVDRAMKHFGTFDPTASAPGIEQTAAVPLAPSPEPPPK"
     gene            complement(2463..6773)
                     /locus_tag="VF516_15265"
     CDS             complement(2463..6773)
                     /locus_tag="VF516_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8109091.1"
                     /translation="MTAIRLEFDAVLDVDAGAAAGSAWDLVPGPSGLPVAFRSFVGGS
                     GQPLRVAAAVSPDMGATAAVHTLLPLVQALKPRCVAMCGVCAGRRGKTQLGDVVAAER
                     LFFHDTGKQLPDHVQQDLTTYKLRDDWKAALEGFDAAAHFRDAAWLQRRPLTSEWREH
                     RALIALRDGVPEPWQAVEPGLGAESWKQIIAALRKRELLAASGSTLTDKGRRAADDLL
                     FEHQGALPDLSPTGAFQPFRVHVAPMGSGARVIEDEAIWSFISRAMRKTLALEMESAA
                     LAELVHRQRQHQLDTVVMKGVMDFADHGRDDQFKAFAARASAECLLWFLRQHVATEAA
                     DSARSAPASSDRLDYRIERQRHASFLGRGAQLARLDRLLVEDEADHWVVVTGGPGMGK
                     SAILTAWLSRREAAGAVVPHHFIRRGEGGWDEPAKIVASLSAQIEACFPAQRDPDARP
                     ESRLADLLARVATHELAPRGDHLVLLIDGLDEYDAPASAYNPLAAFLPQAVPRVRLLC
                     ASRPRHPYLEALEARGGVLIEIDLDDPSAAADNDAIVRAFWRREAAALGLDEQFVDEA
                     VASARGNMQHAVTLRKHLASVPPEQRRVEAIPRGLKALLALLWRRVTNNPVATRGLGV
                     LCAAREPLSLDELGAVARWEDAAQREAFRHAALEFLVETRRDDGTAEYRLHHDSIRAH
                     AMEQLGPAVVRDHHAALAQELATWPAPADAASRRYALRHALTHRAEAGDWAAVGQLAL
                     DVGFLEAKCRELGVQDVEADLARAAERCRARGDAAIGRDLHTLARAVARESHWLHHEP
                     AALAGQLWNRLRRMGWSAEDLDRRVRWPADAPASLRVRHVASRESPALLRDLVGHSSG
                     VYACAITPDGSRVVSAAEDETLAIWDLDTGRALMTLEGHTNWVRDCTVTPDGRRVISA
                     SADETLKVWDLDTGRVLMTLEGHTGWVDGCAVTPDGGRVISASNDGTLKIWNLATGRC
                     LATLEHHIDEVISCAVIAGGERVASASGDGTIKVWDLETGFLRTTIEAHVDGVYTCTV
                     SPDGRRAVSTAQDGILKVWDLETARLLLTIEAHAEAVNRCAVTFDGRRVISTSDDHTL
                     KIWDLETGRLLATLAGHAETVNACAVTRDDRYLVSAAHDRTLKVWDLEASRGATTSEG
                     HAERVDSCAMTPDGQRVISASYDGTLKLWDVETGRLVKTFEGHTSWVQGCAVTPDGRR
                     MISASMDHTLKVWDLRTGRLVKTLKGHTAAVSSCAVTPDGRRVVSVSDDRTFGIWNIM
                     TGHLFKTLERDGDWVGSGALTPDGQCVVSTASDGTLKVWDLATGRLVKTLEGHTELVN
                     RCAVTLDGRRVVSASRDWTLRVWDLATGRSVKTLEGHTDQVHGCAMTPDGRHVISVSD
                     DRTFKIWDLTTYECLATHRGDTGFSSAAATTTVICAGDAAGALWFLDRGPGPPKRSPP
                     "
     gene            complement(6954..7151)
                     /locus_tag="VF516_15270"
     CDS             complement(6954..7151)
                     /locus_tag="VF516_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109092.1"
                     /translation="MGGAGGSTGGDGGDGANKDGQGGNAAGAGGTGTSAAGGGGGGGG
                     VGVIKAYQGQSLPGTVSPPAG"
     gene            complement(7288..>7935)
                     /locus_tag="VF516_15275"
     CDS             complement(7288..>7935)
                     /locus_tag="VF516_15275"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8109093.1"
                     /translation="CPVARAPVSHAQCGSTLDFTPINQYRGDLADAQLREDAVVLIDG
                     NCTGTLIAAAAGPVVLTAGHCVGLGDRALLAFNVEAAPDGDPLVTSGTVIERAGAPDY
                     ALIALDQLPAATPTPLTTRSTERLAIIQHPRGGPKVIAEGDFFAECHGEISYLDLDTL
                     VGSSGAGVLNAQGFLYGVHSDGNCDASGGGDNSGWSAASIVAASAYLQDGDLADR"
BASE COUNT         1250 a         2780 c         2661 g         1244 t
ORIGIN      
        1 gatccggcat cgaccgagat cctgacctcg ctcgtcgaac aatcggccgc gccgctctgt
       61 gcgatcgaga acggccagta cgcgatcgac tccacgggtt tctcgacggt gacgtacgat
      121 cgctggttcg atcagaagca cggcaagctc aacgcgcggc accagtgggt caagctccac
      181 atcgcgtgcg gcaccgtcac gcacgcgatc accgcagcgc gcgtgacgtc cgagggcgac
      241 tgcccgcagc tacccgcgct gctcgcgagc acgatgaagc accacgacgt gcgcgagctc
      301 tcggctgaca aggcgtattc gtcggtcgac aaccacaacg tgatggagtc gctcaacgtc
      361 gaggcgttcg ttccgttcaa ggtcaatgcg gtggtcaacc ccaaggcccc ggtgtggtcg
      421 cgccacctcg tcgagttcca gctcaaccaa gagcggttcc tcgcgcacta ccaccgccgg
      481 agtgcggttg agacgacctt tgccatgatc aaggcccgct tcggcgcgtc ggtcgcgtcg
      541 cgactgccga ccgctcgcgc caacgaagtg ctcgcgatgt gcgtcgcgca caacgcgtgt
      601 tgcctggtca aggccatttt caccgccggg ctcgcgcccg cgttctggca ggacgcgccc
      661 gccgccctgc tctccctggt tcccgaggag caagcatgag cgatctgacc gcgaaggagc
      721 agaaggccgt ccgcaccgcg ctgcggttcc tgcggctgcg cgcgggcgcg tggaagccac
      781 tggtcaaggc gctgcggtac gagtacgaca gcatccagaa ggtggcgagc gggaagaagg
      841 ccgttacccc ggcgctcgcg ttgcgcgtcg ccagattcgt gggcataccg atggatgaac
      901 tcctggccgg ccagtggctg tcgccccgga cgtgcccgca ctgcggccac ccgccggacg
      961 acttcgtgga cgaggacacg atgccggggt gaccgcggcg ccggcccaat ggtatggtcc
     1021 cccatggtcc gaaccgatta cgtcctggcc gtgatggcag ccgcacaggt accgttgagc
     1081 ccggtcgaag tgcagaagct gttcttcatc ctcgataaga aggctggcgc acagcttggc
     1141 gggccattct tcaacttcac gccgtatgcc tacggaccgt tcgatgccgc cgtctaccac
     1201 gaactggaac gcttggccga gcgcgggcta gcgtccatct acggccagag caccacatgc
     1261 aggtacggca tttccgccgc cggattgaag cccggagccg ccgcactggg cgccatcacg
     1321 atgtccggct atatcagcca ggtcgccgaa tatgtccgct cgtcctcgtt cgcccagctg
     1381 atcttggctg tctatgccga atggccggag atgatggcaa acagcatctt ccagggttac
     1441 ccacgatgac gcttctcgtc ggcattcgct gcagcgacgg cgttgttgtt gctgcagatc
     1501 gagcggtgac gctcggctcg atggagcaac caacgatctc tcatcccgcg tgcaagatct
     1561 cggtcctgga tggcagcgtg atagttgctg ggagcggcta cactggcacg ttgcagcgct
     1621 tctgcgatgt tgttgaggac accttgctca actgcgctga ctgcgctggc tatcaacaga
     1681 aacgtccgat cgaaattggc cgcatcttgt gcacggctgg tcgcaaggat tttcagtcga
     1741 caatgatgaa ggacggcata ttcggcgccc tggtcgcatt tccaagcgtg atgggccgcg
     1801 gcccacaact ttgtgagttc cacgaaaagg actttcagcc cgatctaaaa gacgccggca
     1861 catggttcgt ttcgatgggc tacggtcgcc ccatagccga ttcatatctc gcgttcatgc
     1921 gtgacacttt ctggcgcggc ggaatgccga ctctcgcgca tgggatattc tccgcagcgt
     1981 gggtcatgca gcagtccatt agagtctcgc ccggcttcat cgatgaaccg atcgacatcg
     2041 ccgtcctgca gggcgagaag gcgcgcatgc ttgagccacg tgagttgacc taccacatgg
     2101 gcgccgtcga tcgggcgatg aaacacttcg ggacgttcga tccaacggca tccgcaccag
     2161 ggatcgaaca gacggcggcc gtacctttgg caccgagccc agagccgccg ccgaagtagc
     2221 ctgggagcag atcccggatg cagatcccgg ccccggcgcc tcacctgcac atttattcgg
     2281 agcgctgcac atttattcga ggctggaggt caaaaacgtg caaagccgac gctgtccggg
     2341 aaatggccgg attccggccg ggcgtccgag atccggccaa acaccggaca agagacaaga
     2401 gacaagagca cgcaacttgg caacgaaggt cgggttcgga gcgaacgccg cgatcgactg
     2461 gctcacggcg gactgcgctt cggcgggccg gggccccggt cgaggaacca gagtgcgccc
     2521 gcggcgtcgc cggcgcagat cacggtcgtg gtcgcggccg cggacgaaaa gcccgtgtcg
     2581 ccgcgatggg tggccagaca ctcgtaggtc gtgaggtccc agatcttgaa ggtccggtcg
     2641 tccgataccg agatcacgtg tcggccgtcg ggagtcatcg cgcagccatg cacctggtcg
     2701 gtatggcctt cgagtgtctt caccgagcgc cccgtcgcga gatcccagac cctgagcgtc
     2761 cagtccctgg aggccgagac cacgcggcgg ccgtcgaggg tcaccgcgca gcgattcacc
     2821 agctcggtat ggccctcgag cgtcttgacg aggcgaccgg tcgcgaggtc ccagaccttg
     2881 agcgtcccat cgctcgcggt cgagaccacg cactggccat cgggagtcag cgcgccgctg
     2941 cccacccagt cgccatcacg ctcgagcgtc ttgaacaggt ggccggtcat gatgttccag
     3001 atcccgaacg tccggtcgtc ggacaccgag accacgcgcc ggccatcggg agtcaccgca
     3061 cagctcgata ccgccgcggt atggcccttg agcgtcttga ccagacgacc ggtcctgagg
     3121 tcccagacct tgagggtgtg gtccatggac gccgagatca tgcgccggcc atcgggagtc
     3181 accgcgcagc cctgcaccca gctggtatga ccttcgaatg tcttgaccag gcgaccggtc
     3241 tcgacgtccc agagcttgag cgtcccgtca taggacgccg agatcacgcg ctgcccatcg
     3301 ggggtcatcg cgcagctgtc cactcgttcg gcatggcctt cgctcgtggt ggcgccgcga
     3361 ctggcctcga gatcccagac cttgagcgtc cggtcgtggg ccgccgagac caggtatcga
     3421 tcatcgcggg tcaccgcgca ggcgttgacc gtctcggcat ggcccgcgag ggtcgcgagc
     3481 agacgaccgg tctcgaggtc ccagatcttg agcgtgtggt cgtcggaggt cgagatcacc
     3541 cgccggccat cgaaggtcac cgcgcagcga ttcaccgcct cggcatgcgc ttcgatcgtc
     3601 aggagcaggc gagcggtctc gagatcccag accttgagga tcccgtcctg cgccgtggag
     3661 accgcgcgcc ggccgtcggg actcaccgtg caggtataca ccccatcgac gtgggcttcg
     3721 atcgtcgtgc gcaggaagcc ggtctcgagg tcccagacct tgatcgtccc gtcaccggac
     3781 gccgaggcca cgcgctcgcc acctgcgatc accgcgcagc tgatcacctc gtcgatatga
     3841 tgttcgagcg tggcgaggca gcggcccgtg gcgagattcc agatcttgag cgttccgtcg
     3901 ttggacgcgg agatgacgcg cccgccatcg ggagtcaccg cgcagccatc cacccagcca
     3961 gtatggcctt cgagcgtcat gagcacgcgc cccgtgtcga ggtcccagac cttgagcgtc
     4021 tcgtcggcgg acgcggagat gacgcgccgg ccatcggggg tcaccgtgca gtccctgacc
     4081 cagttggtgt ggccttcgag cgtcatgagc gcgcgccccg tgtcgaggtc ccagatcgcg
     4141 agcgtctcgt cttctgctgc cgagaccaca cggctgccat cgggcgtgat ggcacacgcg
     4201 tacactcctg aggaatggcc gacgagatcg cgcagcagcg cggggctctc gcgagatgcg
     4261 acgtgacgca cgcgcaggga cgcgggagca tccgcaggcc atcggacccg ccggtcgagg
     4321 tcctccgcgc tccaccccat ccgccgcagc cggttccaca gctgcccggc gagcgcggct
     4381 ggctcgtggt gcaaccagtg agactcgcgt gccacggcgc gtgcgagggt atggaggtcg
     4441 cgaccgatgg cggcgtctcc ccgcgcgcgg catcgctccg cggcgcgcgc gaggtcggcc
     4501 tcgacatcct ggacgcccag ctcgcggcac ttggcctcga ggaaccccac gtcgagggcg
     4561 agctggccga ccgcggccca gtcgccggcc tcggctcgat gggtgagcgc gtggcgcaag
     4621 gcataccggc ggctggcggc atcggcgggc gccggccacg tcgcgagctc ctgtgccagg
     4681 gcggcgtgat ggtcgcgcac caccgccgga ccgagttgct ccatggcgtg cgcgcggatc
     4741 gagtcgtggt gcaggcggta ctctgccgtg ccatcgtcgc gccgcgtctc gaccagaaac
     4801 tccagggcgg cgtggcggaa cgcctcccgc tgggcagcgt cctcccagcg ggcgaccgcg
     4861 cccagctcgt cgagcgacag cggctcgcgc gccgcgcaca ggacgccgag cccgcgcgtc
     4921 gcgacggggt tgttcgtgac gcggcgccac agcaacgcga gcagcgcctt gaggccccgc
     4981 gggatggcct ccacgcggcg ttgttccggc ggcacgctcg ccaggtgctt tcgcaaggtg
     5041 accgcgtgct gcatgttgcc gcgggcgctc gccaccgcct cgtcgacgaa ctgctcgtcg
     5101 agcccgagcg ccgccgcttc gcgccgccag aacgcgcgga cgatcgcgtc gttgtcggcg
     5161 gccgcggacg ggtcgtcgag atcgatctcg atcagcacgc cgccgcgcgc ctcgagcgct
     5221 tcgaggtacg ggtgccgcgg ccggctcgcg cacaggagcc gcacgcgcgg caccgcctgg
     5281 ggcaggaacg cggccagcgg attgtaggca ctggccggcg catcgtactc gtcgaggccg
     5341 tcgatcagca gcaccaggtg gtcgccgcgc ggcgccagct cgtgggtcgc cacgcgcgcc
     5401 aggaggtccg ccagccgtga ctccggcctc gcgtccggat cgcgctgcgc cggaaaacac
     5461 gcctcgatct gcgccgacag cgaggccacg atcttcgccg gctcgtccca gccgccctcg
     5521 ccgcggcgga tgaagtggtg cggcacgacc gcgccggccg cctcgcgccg cgacagccac
     5581 gcggtcagga tggcgctctt gcccatgccg gggccgccgg tcaccacgac ccagtgatcg
     5641 gcctcgtcct cgacgagcag ccggtcgagc ctcgcgagct gggcgccgcg gcccaggaag
     5701 ctggcgtggc gctgccgctc gatgcggtag tcgaggcgat cgctcgacgc cggtgccgat
     5761 cgcgcgctgt ccgccgcctc ggtcgcgacg tgctggcgca gaaaccacag caagcactcg
     5821 gccgacgcgc gcgccgcgaa cgccttgaat tgatcgtcgc ggccgtggtc ggcgaagtcc
     5881 atcacgccct tcatcaccac cgtgtcgagc tggtgctgcc gctggcggtg gaccagctcg
     5941 gccagcgccg cgctctccat ctccagcgcg agcgtcttgc gcatcgcgcg cgagatgaag
     6001 ctccagatcg cttcgtcctc gatcacgcgc gcgcccgagc ccatcggcgc gacgtgcacg
     6061 cggaacggct gaaacgcacc cgtcggtgac aggtcgggca gcgccccctg gtgctcgaac
     6121 agcagatcat ccgcggcccg gcggcccttg tcggtcagcg tgctcccgga agcggccagg
     6181 agctcgcgct tgcgcagcgc ggcgatgatc tgtttccacg actcggcgcc gaggcccggc
     6241 tcgaccgctt gccacggctc gggcacgccg tcgcggagcg cgatcaacgc gcggtgctcg
     6301 cgccactcgc tggtgagcgg ccggcgctgg agccacgcgg catcgcgaaa gtgcgccgcg
     6361 gcgtcgaacc cctccagggc agccttccag tcgtcgcgga gcttgtacgt ggtcagatcc
     6421 tgctggacgt ggtcgggcag ctgcttgccg gtgtcgtgaa agaacagccg ttcggcagcc
     6481 acgacgtcac cgagctgggt cttgccgcgc cgccccgcgc agacgccgca catcgcgaca
     6541 cagcgaggct tcagcgcttg cacgaggggc aagagcgtat ggaccgcggc ggtcgctccc
     6601 atgtccggcg acaccgcagc ggcgacgcgc aacggctgcc cgctgccgcc gacgaacgag
     6661 cggaacgcga ccggcaatcc gctggggccc ggcacgagat cccacgccga gcctgcagcc
     6721 gcgcccgcat cgacgtcgag caccgcgtcg aactccagcc ggatggcggt caagatcacg
     6781 acatcggcgc gccgtatccc tgcatcccaa tcttttgcga cggtctgtgc agtccttgtg
     6841 cccacaggct ccgagtgacg atcggtcgcc ctgcgacgcg tgtcaacacg ccgcgccgcc
     6901 atgcggatcg acgagatccg gacgatgacg ccgagcaccg tgtcgacgtc ctgctagcct
     6961 gccggcggcg acaccgtgcc gggcagcgac tggccctggt aggccttgat cacgccgacg
     7021 ccaccgccgc cgccaccgcc accggccgcc gaggttccgg ttccacccgc gccggcggca
     7081 ttgccgccct ggccatcctt gttcgcgcca tcgccaccgt ccccgccggt gctgccgccg
     7141 gcacctccca tggcagccat cttcggatcc agcggcgcat cgctgccatt gtggcccacg
     7201 agtccgccgc tgccttcccc gccgctgcca ccgttcgcga agatcttggc gccgccgcca
     7261 tctgtgaccc cagacacttt tgctcggtca gcgatccgcg agatcgccgt cctgcaagta
     7321 ggcggacgcc gcgacgatgc tcgccgcgct ccagccggag ttgtcgccgc cgccgctggc
     7381 gtcgcagttg ccgtcgctgt ggacgccgta caggaagccc tgcgcgttga gcacgcccgc
     7441 acccgagctg ccgaccaggg tgtcgagatc gaggtacgag atctcgccgt ggcactcggc
     7501 gaagaagtcg ccctcggcga tcaccttggg gccgccgcgc ggatgctgga tgatcgcgag
     7561 ccgctcggtc gaccgcgtgg tcagcggggt cggggtcgcg gccggcagct gatcgagcgc
     7621 gatcaacgca tagtccggcg cgccggcgcg ctcgatcacg gtgccgctgg tgaccagcgg
     7681 gtcgccgtcg ggggcggcct cgacgttgaa tgcgagcagc gcgcgatcgc ccaggccgac
     7741 gcagtgcccg gcggtgagca ccaccgggcc ggcggcggct gcgatcagcg tgccggtgca
     7801 gttgccgtcg atcaggacga cggcgtcctc gcgcagctgc gcgtcggcga ggtcgccgcg
     7861 atactggttg atcggcgtga agtcgagcgt gctgccgcac tgcgcgtgcg agaccggggc
     7921 gcgcgccacc gggca
//
LOCUS       DASVOR010000774         1655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_212229,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000774 DASVOR010000000
VERSION     DASVOR010000774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1655
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212229"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..>1655)
                     /locus_tag="VF516_15280"
     CDS             complement(204..>1655)
                     /locus_tag="VF516_15280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEX8109094.1"
                     /translation="FGTPIDQIGWVSTGYMMANIVVIPMTGWLQRRFGFRRYFSTSVL
                     IFTVASALCGLSWNLPSLVLFRVLQGIGGGAIIPSSQAILFARYPRTQHGMAAALFGL
                     GAITGPLLGPTIGGYMIDWASWHWIFLVNVPIGLTVAFLIPRVLQEPGFTPDTTRIDA
                     TGIGLLAIGMASLQYVLEEGNREGWTDSTRILVLGGLAAIAILTFIVHELEAPHPVVD
                     LRVFKNRNYAAGTGLNFLLGLAVFGAAYLFSLFCGAVMRYQALDIGRVFLLAGASQIF
                     LMPVIGKIANRLDPRLMLVWGVGFTTLSQFVASHLYSDAAFIDLVVPNLIRSFGLAFV
                     FIPVSVAALSDLPLAQRGNATGLFNLTRELGGSLGTAWMGKVVADGITTHSSQLGEHV
                     TPYNPIAQAQWLRIARGGMDPPTMLAGRVLREAMVLSFEDGFRITMTAIGLGIVMVML
                     LKRSAGHGAPTLPVAVAREGSGDAHRAPPSGAH"
BASE COUNT          283 a          574 c          591 g          207 t
ORIGIN      
        1 acgtcgcgtc gggccggtcg cgggcccagc gcaccagcca gtcgccgacg cagcgcgccg
       61 gtgcgccggg cgtggcgggc gattccagga ccaggcctcc cggtgtgcgg tggctgcgaa
      121 cctcggctgg gagcagctcg atcatggccg ccatgacccg acgatagctc gctccgcggg
      181 cggcgcgcga gccgatccgc cgatcagtgc gcgccgctcg ggggcgcgcg atgggcgtcg
      241 ccgctgcctt cgcgtgccac ggcgacaggc agcgtcgggg cgccgtggcc ggcggaccgc
      301 ttgagcagca tcaccatcac gatgccgagg ccgatcgcgg tcatcgtgat ccggaagccg
      361 tcctcgaacg acagcaccat ggcctcgcgc agcacccggc cggcgagcat ggtcggcgga
      421 tccatcccgc cgcgggcgat cctgagccac tgggcctggg cgatcgggtt gtagggcgtg
      481 acgtgctcgc cgagctgcga cgagtgggtg gtgatgccgt cggcgacgac cttgcccatc
      541 cacgccgtgc cgagcgagcc gccgagctcg cgggtcaggt tgaacagccc ggtcgcgttg
      601 ccgcgctgcg cgagcggcag atcggacagc gccgcgacgc tgaccgggat gaacacgaac
      661 gccagaccga acgagcggat caggttgggc accaccagat cgatgaacgc ggcgtccgag
      721 tacaggtgcg acgccacgaa ctgcgacagc gtcgtgaacc cgacgcccca caccagcatc
      781 agccgcgggt cgagccggtt ggcgatcttg ccgatcaccg gcatcaggaa gatctgcgac
      841 gcgccggcga gcaggaacac ccggccgatg tcgagcgcct ggtagcgcat caccgcgccg
      901 cagaacagcg agaacaggta cgccgcgccg aacaccgcga gcccgagcag gaagttgagc
      961 ccggtgcccg cggcgtagtt gcggttcttg aacacccgca gatcgaccac cgggtgaggg
     1021 gcctcgagct cgtggacgat gaacgtgagg atcgcgatcg cggcgagccc gccgagcacc
     1081 aggatccgcg tgctgtcggt ccagccctcg cggttgccct cctcgagcac gtactgcagc
     1141 gacgccatcc cgatcgcgag caggccgatg ccggtcgcgt cgatccgcgt ggtgtcgggc
     1201 gtgaacccgg gctcctgcaa gacgcgcggg atcaggaacg cgacggtcag gccgatcggc
     1261 acgttgacca ggaagatcca gtgccagctc gcccagtcga tcatgtagcc gccgatcgtc
     1321 ggtccgagca gcgggccggt gatcgcgccg agcccgaaca gcgccgccgc catgccgtgc
     1381 tgggtccgcg ggtagcgcgc gaacaggatc gcctgcgagc tcgggatgat cgcgccgccg
     1441 ccgatgccct gcagcacgcg gaacagcacc agcgacggca ggttccacga caggccgcac
     1501 agcgcgctcg cgaccgtgaa gatcaggacc gaggtcgaga agtagcgccg gaacccgaac
     1561 cggcgctgca gccagccggt catcgggatc acgacgatgt tggccatcat gtagccggtc
     1621 gacacccagc cgatctgatc gatcggcgtg ccgaa
//
LOCUS       DASVOR010000775         3367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_241260,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000775 DASVOR010000000
VERSION     DASVOR010000775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241260"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF516_15285"
     CDS             <1..237
                     /locus_tag="VF516_15285"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX8109095.1"
                     /translation="PPAKVDRYTDDQLYFLAYAQSWCDKQTPEVLETRAHSNPHSPPM
                     WRVNGVIVDQPEFGRAFHCAAGAAMNPGKQCSVW"
     gene            complement(239..1003)
                     /locus_tag="VF516_15290"
     CDS             complement(239..1003)
                     /locus_tag="VF516_15290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109096.1"
                     /translation="MRTVSLLALAIAAVGCGGSQPLPRTNLHGYAARLEAADRYDQLA
                     DAHRRAAGPPDTVINPDNYQCGDINLDEELTSGGKPLVRFGAHSVPCWNPAEEHAQRQ
                     RLAAERLHRRAQDERHAAGRLIEAELEACRGISPAEIEHSPFAHRQEIAEVIPHRERG
                     EIRGVRIVFKPVLGLSPAWMEQAIACHRARFERLGEPPSYLADDPTLVSGAAATVSLH
                     RGHLEVLVEARDDVAAHVALARAQDLVRPRTQTAVR"
     gene            complement(1072..1890)
                     /locus_tag="VF516_15295"
     CDS             complement(1072..1890)
                     /locus_tag="VF516_15295"
                     /inference="COORDINATES: protein motif:HMM:NF025389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4239 domain-containing protein"
                     /protein_id="HEX8109097.1"
                     /translation="MPRWVRTFLHLSPVVGGVSVASVLGLLVFREFVPLDVLRSAEDA
                     IGNYLQTVGGIYAVLLAFVVYVVWGQFNEARGYLDREATALVDLHRTASALPSRSRTE
                     IQRGLRAYVDAVLRDEWPAMACRDEATIDRVGELLEQVWIAIHACRPCNDCQHTVYGE
                     VLSRFNQLTDLRTSRLSSARARIPVAMTILLYNGAVLTVGSVYLLAFDRWWLHAIVTA
                     ALAGAIAHILFLIRDLDDAFDGDWQVARTPFEHARKNFERAAHVVDVDAVLPAG"
     gene            2071..>3367
                     /locus_tag="VF516_15300"
     CDS             2071..>3367
                     /locus_tag="VF516_15300"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8109098.1"
                     /translation="MTQREKRRTRAQDAASSLGDFIRDRQQDILTEWLAEVRLLPIAS
                     ALDRPALIDHVPDVLDRIAAMADCLSRGEAPEPPKQEADLHAKIRLEEGFDLPQVVSE
                     FTILRDCITRLWEQSVVNPSHIDELRVVNKAIDNAVGASVDRFTRARDRTLIALDRIA
                     SEALESKSLDDLLQRLLKVFVEFTAAVDTAAILIREGDRLRVRAALGLNRESEVGLTM
                     KIGEGFAGTVAKERRAIGIAAGTAVPFIRSEILRSAGIRALYGVPLIDGHGLIGVAHM
                     GSLTANEFSMQDKRLLNAMANRATSAIFQHLLREAAEKSAAELSTILESIPAAVFIGN
                     ERAVTRATRAGLDLLGYDDSDQLAWPPVVSLVEALDMRDAGSGARLDVGNGPGRTAVR
                     GVTYQRDVLIHDHTAAKDAIVHAVAAPIVRDGNVIGAVAV"
BASE COUNT          545 a         1158 c         1187 g          477 t
ORIGIN      
        1 ccgcccgcca aggtcgaccg ctacaccgac gatcagctgt acttcctggc ctacgcccag
       61 tcgtggtgcg acaagcagac gcccgaggtc ctcgagaccc gcgcgcactc caacccgcac
      121 tcgccgccga tgtggcgggt caacggcgtg atcgtcgatc agcccgagtt cggccgcgcg
      181 ttccactgcg ccgccggggc ggcgatgaac cccggcaagc agtgcagcgt gtggtgatct
      241 agcgcaccgc ggtctgggtc cggggccgca ccaggtcctg ggcgcgggcg agcgcgacgt
      301 gcgcggcgac gtcgtcgcgg gcctcgacca gcacctcgag gtggccgcgg tgcagcgaga
      361 ccgtcgcggc cgcgccggac accagcgtcg ggtcgtccgc caggtagctc ggcggctcgc
      421 cgagccgctc gaaccgtgcg cggtgacagg cgatcgcctg ctccatccac gccggcgaca
      481 ggccgagcac cggcttgaac acgatgcgca cgccgcggat ctcgccgcgc tcgcggtgcg
      541 ggatcacctc ggcgatctcc tgtcggtggg cgaacggcga gtgctcgatc tccgccgggg
      601 agatcccgcg acacgcctcg agctcggcct cgatcaggcg gcccgccgcg tgccgctcgt
      661 cctgggcacg gcggtgcagg cgctcggccg cgagccgctg gcgctgggcg tgctcctcgg
      721 cggggttcca gcacggcacc gagtgcgcgc cgaaccgcac cagcggcttg ccgccgctgg
      781 tcagctcttc atccaggttg atgtcgccgc actggtagtt gtcggggttg atcaccgtgt
      841 caggcgggcc cgccgcgcgg cgatgggcat ccgcgagctg atcgtaccgg tccgccgcct
      901 ccaggcgggc ggcgtagccg tggaggttcg tcctcggcag cggctgcgag cctccgcagc
      961 cgacggccgc gatcgcgagc gcgagcaggg aaacggtccg catgatccga cgcccgtgca
     1021 tgtcctgcgc cgggggcggt cggggcattt ctgcgggcgg cggtcgcccg gctagccggc
     1081 cgggagcacc gcgtcgacgt cgaccacgtg ggcggcccgc tcgaagttct tgcgggcatg
     1141 ctcgaacggc gtccgggcga cctgccagtc gccgtcgaac gcgtcatcga gatcgcggat
     1201 caggaacagg atgtgcgcga tcgccccggc gagcgcggct gtcacgatcg cgtgcagcca
     1261 ccagcggtcg aacgccagca ggtagaccga gcccaccgtg agcacggcgc cgttgtagag
     1321 caggatcgtc atcgccaccg ggatccgcgc gcgcgccgag ctgagccggc tggtccgcag
     1381 gtcggtgagc tggttgaacc gcgacagcac ctcgccgtag acggtgtgct gacagtcgtt
     1441 gcacggccgg cacgcgtgga tcgcgatcca gacctgctcg agcagctcgc cgacccggtc
     1501 gatcgtcgcc tcgtcgcggc acgccatcgc cggccactcg tcgcgcagca ccgcgtcgac
     1561 gtacgcccgc agcccgcgct ggatctcggt gcgcgaccgc gacggcagcg cgctcgccgt
     1621 ccggtgcagg tcgaccagcg ccgtggcctc gcgatcgagg tagccgcgcg cctcgttgaa
     1681 ctggccccac acgacgtaga ccacgaacgc gagcagcacc gcgtagatcc cgccgaccgt
     1741 ctgcaggtag ttgccgatcg cgtcctcggc ggagcgcagg acgtcgagcg gcacgaactc
     1801 gcggaacacg agcagcccca ggacgctcgc cacggagacg cccccgacga ccggagacag
     1861 gtgaaggaag gtgcggaccc agcgcggcat gggccggcag tgtagcgagc ttgccggcga
     1921 aggtgagcgc cgacgccgca ccgtgcgcca ttggcacagg cacggcgcca cctgcgctgg
     1981 cgcacatcag ggtatcgccg gaccgtggtt cgcgtgcgct gagggatcgc cgcatgcaag
     2041 gaacctcggg agctacgccg gaaacacagg atgacccagc gcgaaaagcg tcgaacgagg
     2101 gcgcaggacg cagcatcgag tcttggcgac ttcattcgcg accgtcaaca agacatcctg
     2161 acggagtggc ttgccgaggt acgcttgctg cccatcgcga gcgcactgga tcggccggcg
     2221 ttgatcgacc acgtcccgga tgtgctggat cgcatcgcgg cgatggcaga ttgcctctcg
     2281 aggggggagg cgccggagcc gccgaagcaa gaagcagatc tgcacgcgaa gatacgactg
     2341 gaggaaggct tcgatctgcc gcaggtggtc agcgagttca cgattttacg cgactgcatc
     2401 acgcggctct gggagcagag cgtcgtgaac ccgtcgcaca tcgacgagct gcgggtcgtc
     2461 aacaaggcga tcgacaatgc ggtcggtgca tccgtcgatc gcttcactcg cgcacgagat
     2521 cggacgctca tcgcactcga tcggatcgct tccgaggcgc tcgagagcaa gagcctcgac
     2581 gatctcttgc agcggctcct caaggtgttc gtcgagttca cggccgccgt cgataccgcc
     2641 gcgatcctga ttcgcgaagg cgaccggtta cgagtccgcg ccgccctcgg actcaaccgc
     2701 gagagcgaag tcggcctcac gatgaagatc ggagaaggat tcgcaggcac ggtcgccaag
     2761 gagcgccggg cgatcgggat cgcggccggc actgcggttc cgttcatccg gagcgagatt
     2821 cttcgctcgg ccggtatccg ggctctgtac ggcgtcccgc tcatcgacgg gcacggcctg
     2881 atcggcgtcg cgcacatggg ctcgctgact gccaacgagt tctcgatgca ggacaagcgc
     2941 ctgctcaacg cgatggccaa ccgcgcaacg agtgcgatct tccagcactt gctccgggaa
     3001 gctgccgaga aatccgcggc agagctgtcg acgatcctcg aaagcatccc cgcggcggtc
     3061 ttcatcggaa acgagcgagc ggtcacgcga gccactcgcg cagggctcga cctgctgggt
     3121 tacgacgact ccgaccagct cgcgtggcca ccggtggtga gcctcgtcga ggcgctcgac
     3181 atgcgcgacg cgggtagcgg cgcgcggctg gacgtcggga acggtccggg acgcaccgcg
     3241 gtgcgaggcg tcacctatca gcgagacgtg ctcatccacg atcacacagc cgcgaaggac
     3301 gcgatcgtgc atgccgtggc ggcaccgatc gtgcgcgacg gcaacgtgat cggcgcggtc
     3361 gcggtgg
//
LOCUS       DASVOR010000776         2875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_65924,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000776 DASVOR010000000
VERSION     DASVOR010000776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65924"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VF516_15305"
     CDS             <1..497
                     /locus_tag="VF516_15305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109099.1"
                     /translation="WIPAFALGAAAAAVALLVVRPPATDVAVLDEIVGDHLRIVGAPR
                     PLDVETNDMHNVKPWFTGKLDFVPPVNFVGDDEFHLRGGSLAVFQGHKAAAFVYQRRL
                     HTITLLVHADAAARQRAETTSRGFHVISWASGGFGMALVSDIGWDDLRQLEARLGSAS
                     GEKE"
     gene            782..1141
                     /locus_tag="VF516_15310"
     CDS             782..1141
                     /locus_tag="VF516_15310"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8109100.1"
                     /translation="MVKGAARAAQLRRRLGARIRALRDDRGLTQEVIANRIGVSPKYV
                     SQLECGQRSPSWETVVAIAHYGFETGVAALMFGIDEDLEADVQDLSEVLAGRTKEARR
                     DLLQAVRLMLRAGAGSK"
     gene            1324..1683
                     /locus_tag="VF516_15315"
     CDS             1324..1683
                     /locus_tag="VF516_15315"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8109101.1"
                     /translation="MVKGAARAAQFRRHLGARIRALRDARKLTQEVIAGRIGVSPKYV
                     SQLECGQRSPSWETVVAIAHHGFDIKVAALMFGIDEDIEVEVQDLSEVLAGRPKEARR
                     DLLHAVRLMLRAGAGSQ"
     gene            1800..>2875
                     /locus_tag="VF516_15320"
     CDS             1800..>2875
                     /locus_tag="VF516_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8109102.1"
                     /translation="MPHPNQYKSNLRDLTFLLFEQFKLGDLLGKAPYANWGKDEVLAV
                     LEEAYGWVQKHLGPYNRSGDDEGCRLIDGQVHVPAGFKEAWKALYEAGWRTLAVDEAH
                     GGQAGPLTLALMVEEFMCGANTSFNMYPALTHGAADVILAFGTPAQQATYVPNLFNGK
                     WAGTMCLTEPHAGSDVGSASTTATRRSDGKYDIRGVKIFISGGDHDMTDNIVHMVLAR
                     TPDAPPGTKGLSLFIVPKRRLDGTPNDVTVGGIEHKMGIRASTTAMLNFGDGGGCIGE
                     LVGTAEQKGMSQMFHLMNSARIGVGIQSLALASSAYLNALDYARERKQGSSIKQWKDA
                     TAPRVPIIDHPDVRRMLLDMKARV"
BASE COUNT          473 a          997 c          976 g          429 t
ORIGIN      
        1 cctggattcc cgcgttcgcg ctcggcgccg ccgccgccgc ggtcgccctg ctcgtcgtcc
       61 gccccccggc caccgacgtc gccgtgctcg acgagatcgt cggcgaccac ctgcggatcg
      121 tcggcgcgcc gcgcccgctc gacgtcgaga ccaacgacat gcacaacgtc aagccgtggt
      181 tcaccggcaa gctcgacttc gtcccgccgg tcaacttcgt cggcgacgac gagttccacc
      241 tgcgcggcgg ctcgctcgcc gtgttccagg gccacaaggc ggcggcgttc gtctaccagc
      301 gccggctcca caccatcacg ctcctggtcc acgccgacgc cgcggcccgc cagcgcgccg
      361 agaccacgag ccgcggcttc catgtcatca gctgggcgag cggcggcttc ggcatggccc
      421 tggtctccga catcggctgg gacgacctcc gccagctcga ggcccgcctg ggcagcgcct
      481 cgggcgagaa ggagtgaccg ccccgcatcg tccgcacgac gccctggtcg ctcgccgagc
      541 atgccagcgt catccccacg aagggcaaga atgagcgcat gcggaagcca agagtgggcc
      601 atgaagctcc caggcggcca tgcggtgatg cgtgacgagc tgtcagtagg aagcactggt
      661 cagcttcact gctcgacgct ctatcgccga ggtgcgtccc tgctgtcagt agaagttgcg
      721 ctgatcttct gagctttcgg taggttatcg ccatcgccgg gcacgcctac gctggccgtc
      781 gatggtcaag ggcgccgctc gcgcagctca gctccggcgc cgcctcggtg cgcggatccg
      841 agcgctccgt gacgatcgcg ggctcacgca ggaggtcatc gcgaaccgca tcggcgtctc
      901 gccgaagtac gtgagccagc tcgagtgcgg ccagcggtcc ccgagctggg agaccgtggt
      961 ggcaatcgcg cactacggct tcgagaccgg ggtcgccgcg ctgatgttcg ggatcgacga
     1021 ggatctcgag gccgacgtac aggatctcag cgaggtgctc gccggccgga cgaaggaagc
     1081 ccggcgcgac ctgctgcagg ccgtgaggct gatgctgcgc gcgggcgcag gatcgaagta
     1141 gtggggccgg atctggcggc cgtggggtgg tgcaggacga gctgtcagta ggaagcatct
     1201 ggtcagctca ctgctcgacg ctctattgcc cggaggcgcg tctactcgca gtagaagtcg
     1261 ccctgatgtt ctgaactttc agtaggttat cgccatcacc cggcgcgcct aggctgaccc
     1321 tcgatggtca agggcgccgc tcgcgcggca cagtttcggc gtcacctcgg ggcgcggatc
     1381 cgagcactcc gggacgcacg caagctcacg caggaggtga tcgcaggccg catcggcgtc
     1441 tcgccgaagt acgtcagcca gctcgagtgc ggccagcgat cgccgagctg ggagaccgtg
     1501 gtggcgatcg cgcaccacgg cttcgacatc aaggtcgccg cgctgatgtt cgggatcgac
     1561 gaggacatcg aggtcgaggt ccaggatctg agcgaggtgc tcgccggccg accgaaggaa
     1621 gctcgccgcg atttgctgca tgccgttcgg ctgatgctgc gcgccggcgc aggctcgcag
     1681 tagcggcgaa gtcgcggcgc cgctgggcgg atcgggtgca tcggcccaca tcgaccttga
     1741 cgcgtcgtgt tagcggcgtc ataaaatgaa tgacggttca ttcaccgcga ggtccgacga
     1801 tgccgcaccc caatcagtac aagtccaacc tccgcgatct gacgttcctc ctgttcgagc
     1861 agttcaagct cggcgacctg ctcggcaagg cgccgtacgc gaactggggc aaggacgagg
     1921 tgctcgcggt gctcgaagag gcctacggct gggtccagaa gcacctcggg ccctacaacc
     1981 gctccggcga cgacgagggc tgccggctga tcgacggcca ggtccacgtc cccgccggct
     2041 tcaaggaggc gtggaaggcg ctgtacgagg cgggctggcg gacgctcgcc gtcgacgagg
     2101 cgcacggcgg ccaggccggg ccgctgacgc tcgcgctgat ggtcgaggag ttcatgtgcg
     2161 gcgcgaacac ctcgttcaac atgtacccgg cgctgaccca cggcgcggcc gacgtgatcc
     2221 tcgcgttcgg cacgcccgcg cagcaggcga cctacgtgcc gaacctgttc aacggcaagt
     2281 gggccggcac gatgtgcctg accgagccgc acgccggcag cgacgtcggc tcggcgagca
     2341 ccacggcgac ccgccgctcc gacggcaagt acgacatccg cggggtcaag atcttcatct
     2401 ccggcggcga ccacgacatg accgacaaca tcgtccacat ggtgctcgcc cgcaccccgg
     2461 acgcgccgcc cggcaccaag ggcctgtcgc tgttcatcgt gcccaagcgc cgcctcgacg
     2521 gcacgcccaa cgacgtcacg gtcggcggca tcgagcacaa gatggggatc cgggcgtcga
     2581 ccaccgcgat gctcaacttc ggcgacggcg gcggctgcat cggcgagctg gtcggcaccg
     2641 ccgagcagaa gggcatgtcg cagatgttcc acctgatgaa cagcgcgcgg atcggcgtcg
     2701 gcatccagag cctggcgctc gcgtcgtcgg cgtacctcaa cgcgctcgac tacgcccgcg
     2761 agcgcaagca gggcagctcg atcaagcagt ggaaggacgc gaccgcgccg cgcgtcccga
     2821 tcatcgacca ccccgacgtc cgccgcatgc tgctcgacat gaaggcgcgg gtcga
//
LOCUS       DASVOR010000777         2291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_299986,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000777 DASVOR010000000
VERSION     DASVOR010000777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299986"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VF516_15325"
     CDS             complement(<1..309)
                     /locus_tag="VF516_15325"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8109103.1"
                     /translation="MRRRQAGFTLTEMMVVVTIIGILVTMAIVYMRGHVRPIDVANRV
                     GDLVREASRRAVALGPVRANVAVTMGKARTQISAAHTDRTLPGSSPPITFTTVTFTLW
                     R"
     gene            complement(306..1478)
                     /locus_tag="VF516_15330"
     CDS             complement(306..1478)
                     /locus_tag="VF516_15330"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8109104.1"
                     /translation="MTRTRQRGFTLIELMVALLVSSLLVGMILAIFSRMSLAYRSQQQ
                     ISGVQQILAAARAAIELDAKQAGFGMPQGFKTAVPLLTNPQSPVQVTNNSNNAGPDQI
                     AFFYADTSTQAVLTQPSPVTLTGAVSFDDVSAFSALMLVVLTTASTASNPLSATSPDI
                     ATYDACVLRLATVNVPGKQVTFDTTAPWGTSFAHCTIIVPGRTMMYRFVAHAYRIDTT
                     TPARAQLGALQQSVTGGLLSNVPPGNLEVWKDVAYGFSDLQTAVRLYDRDAPANVDGD
                     DDVNRNWFSGENQGAVTTLTTPPANTALLGMTISLVAHTERNVEGIATTATPNLIALP
                     ALGGPPIPDHNSLSDRASTALPTAPPWPDPLLPGQRIYRWTSFQVDFRNLGVGVGL"
     gene            complement(1475..1987)
                     /locus_tag="VF516_15335"
     CDS             complement(1475..1987)
                     /locus_tag="VF516_15335"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8109105.1"
                     /translation="MGACSETPARSTSRPSPARRAGRRQRGFTLLELLITLSVTTIGL
                     IGLLALHLSIARGNDGASRAAEAEQITVTALESLRAQSPTGMMKTLTGNSATLPPVTA
                     LPYTVLGRNNQPYSVTRSVTALTATSPSLWLIRVVTSWGEGGAAISLPGAPLNHSIAL
                     EVIRTIEEQL"
BASE COUNT          339 a          758 c          829 g          365 t
ORIGIN      
        1 ccgccacagc gtgaaggtga cggtcgtgaa cgtgatcggc ggggacgagc ccggcagcgt
       61 gcggtccgtg tgggcggcgg agatctgggt ccgcgccttg cccatcgtga ccgcgacatt
      121 ggcgcgcacc ggaccgagcg ccaccgcacg gcgacttgcc tcacgcacca ggtcgccgac
      181 ccggttcgcg acgtcgatcg gccggacgtg acctcgcatg tagacgatcg ccatggtgac
      241 caggatcccg atgatggtga ccaccaccat catctcggtc agcgtgaacc ccgcctggcg
      301 ccgcctcaca gtccgactcc gacccccagg ttgcgaaagt cgacctggaa gctggtccag
      361 cggtagatgc gctggcccgg cagcagcgga tcgggccacg gcggcgcggt cggcagcgcg
      421 gtcgacgcac ggtcgctgag cgagttgtgg tcggggatcg gggggccccc cagggccggc
      481 aaggcgatca ggttcggcgt cgccgtcgtg gcgatgccct cgacgttgcg ctcggtgtgg
      541 gcgaccaggc tgatcgtcat gccgagcagc gcggtgttgg ccggtggcgt cgtgagcgtg
      601 gtcaccgcgc cctggttctc gccggagaac cagttgcggt tgacgtcgtc gtcgccgtcg
      661 acattggcag gagcatcgcg gtcgtagagg cgcaccgcgg tctggagatc gctgaagccg
      721 tacgccacat ccttccagac ctcgaggttg cccgggggca cgttgctcag caggccgccg
      781 gtcaccgact gctgcagggc accgagctgg gcgcgcgccg gtgtcgtcgt atcgatgcgg
      841 tacgcatgtg cgacgaagcg gtacatcatc gtgcggcccg ggacgatgat cgtgcaatga
      901 gcgaacgacg ttccccacgg cgccgtcgtg tcgaacgtga cctgcttgcc cggcacgttg
      961 acggtcgcga gcctcaggac gcaggcgtcg taggtcgcga tgtcggggct cgttgcggac
     1021 agaggattcg acgcggtgga ggcagtcgtc aagacgacga gcatgagggc gctgaacgcc
     1081 gacacgtcat cgaagctgac ggcgccggtc agcgtcaccg ggctgggctg ggtcagcacc
     1141 gcctgggtcg acgtgtcggc atagaagaac gcgatctgat cgggtcccgc gttgttgctg
     1201 ttgttggtga cctggaccgg ggattgcggg ttggtcagca gggggaccgc ggtcttgaag
     1261 ccctgcggca tcccgaaccc tgcttgcttg gcgtcgagct cgatcgccgc gcgcgccgcg
     1321 gcgaggatct gctggacccc ggagatctgc tgctggctgc ggtaggccag cgacatccgc
     1381 gagaagatcg ccaggatcat cccgaccagc agggagctca cgagcagcgc gaccatcagc
     1441 tcgatcaagg tgaagccgcg ctgcctggtg cgggtcatag ctgctcctcg atcgtgcgaa
     1501 tgacctccag cgcgatcgag tgattgagcg gagcaccggg caagctgatc gccgcccccc
     1561 cctcgcccca gctggtcacg acccggatca gccacaggct cggcgaggtt gcggtcagcg
     1621 ccgtgaccga tcgcgtgacg ctgtagggct ggttgttgcg gcccagcacg gtgtacggca
     1681 gcgcggtgac cggtggaagc gtcgctgaat tgccggtcag cgtcttcatc atgccggtgg
     1741 gcgactgcgc gcgcagcgat tccagcgcgg tcacggtgat ctgctcggcc tccgcggccc
     1801 ggctcgcgcc gtcgttgccg cgcgcgatgc tgaggtgcag cgcgagcagc ccgatcaggc
     1861 cgatcgtcgt gacgctcagc gtgatcagca gctcgagcag ggtgaacccc cgctggcgcc
     1921 ggcccgcgcg acgcgccggc gagggtcgcg acgtagatcg cgccggcgtc tccgaacagg
     1981 cacccatcac ggccgagctt accgcgacgt ggaagtccct cgtgaaattt gtgccggccc
     2041 cgggttgagc tcgattcccg agcttgattc ccgagatcgc gctgccggtg tcgcaggtgc
     2101 gggtgccgat gccgtccagg gtcgctaccc gagcgtgctc accgcggcca gccgtcggcg
     2161 tcgagctcga tcagcccacc gccgcccaca tcgccgcgcg ccgcgagctc ggccagcgcg
     2221 tcgcgcagcg tcgggacccg gtgactcccc gagggcgcgt cggtccggac cgcggttccg
     2281 gatccgccgg t
//
LOCUS       DASVOR010000778         2097 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_445717,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000778 DASVOR010000000
VERSION     DASVOR010000778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_445717"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1164
                     /locus_tag="VF516_15340"
     CDS             235..1164
                     /locus_tag="VF516_15340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109106.1"
                     /translation="MVAIALVLGGSAAHAATGQSASRHKRAKRPVEAAPAPREDSAEA
                     DAAAPTDAKQVALETADAAGDSDDRDTGKDTGKLVRSAPAPADRGWQVAIGPYLWASS
                     VDAHISLGGSVSTGLDIGFIPLVQHARYGAEILGEVRHGRFALYGDLMYGAEAVTGST
                     SVASVMVTLSGNASSLLVDGAAGYQLIGDEDAVFSLEARAGIRYQRTEVNGQLAIAGF
                     TLQSPDAVDDGSDAIVGTRAVFRPARWVFCSGVFDLGVFGSSDRTWSASADASLRVSS
                     HFLVSAGWRTLTMDRAQVSLSMQGPRAAVQLVF"
     gene            complement(1204..1857)
                     /locus_tag="VF516_15345"
     CDS             complement(1204..1857)
                     /locus_tag="VF516_15345"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8109107.1"
                     /translation="MHGLPLVIAAVLAAGCADRGGHDRLLQPGSAELQQRAPEAFRVR
                     LDTTRGPIVLEVQRAWAPHGVDRFHALVRAGYYDDAAIFRVRAGVFAQFGIHGEPAIA
                     RRWRTRTIPDDPRVLSNTRGTLAYAFKDPDGRTTQVFINLRDNHAAFDAEPFVPFARV
                     IEGMDVADALYAGYGERAGGGIRAGKQDPVFEGGNAYLRRDFPDLDYIQRATIEPGR"
     gene            complement(1866..>2097)
                     /locus_tag="VF516_15350"
     CDS             complement(1866..>2097)
                     /locus_tag="VF516_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109108.1"
                     /translation="SHRSGLRPHAPVVAGGGVVGTTVPDDATWAASDLEAGDVVVFSA
                     LTVHCALDNVTADRLRVSVDYRYRPRPSPALS"
BASE COUNT          295 a          743 c          771 g          288 t
ORIGIN      
        1 ggcgatgcac aacctgtgca gccgggtgga gcaacgacgc agcgcaccgg gcggccttcc
       61 gtcgagccag cccgtcgatg aagccccgcc gtgggaagcg gcgccccagg gtgtcgcccg
      121 tggtagccac gcggatcctc ctgccgtgtt ccggaattgc gcacttggcc ggcaaaacgc
      181 gtggctgccg gtttgcagct cgcatcgcgc atgcgtgcgc acactccagg tctcgtggtt
      241 gccatcgcgc tcgttctcgg cgggtctgct gcgcatgctg cgaccggtca gagcgcgagc
      301 cgtcacaaga gagcgaagcg accggtcgag gccgcgcccg cgccgcgcga ggacagcgcg
      361 gaggcagacg ccgccgcgcc gaccgacgcg aagcaggtcg cgctcgagac ggcagacgcc
      421 gccggcgaca gcgacgacag ggacaccggc aaggacaccg ggaagctcgt gaggtccgcg
      481 cccgcgccgg cggaccgcgg ctggcaggtc gcgatcggac cgtacctgtg ggcgtcctcg
      541 gtggacgccc acatctcgct cggcggttcg gtgtccaccg gcctggacat cgggttcatc
      601 ccgctcgtgc agcacgcccg gtacggcgcc gagatcctcg gcgaggtccg gcacggccgg
      661 ttcgcgctct acggcgatct catgtacggc gccgaggccg tgaccggctc gaccagcgtc
      721 gcgtccgtga tggtgacgct cagcggcaac gcgagcagct tgctggtcga cggcgcggcc
      781 ggctaccagc tgatcggcga cgaggatgcg gtgttctcgc tggaggcgcg ggccgggatt
      841 cgctaccagc gcaccgaggt caacggccag ctcgccatcg cgggcttcac cctgcagagc
      901 cccgacgcgg tcgacgacgg cagcgatgcg atcgtcggca cgcgcgcggt gttccggccc
      961 gcccgctggg tgttctgctc cggcgtgttc gacctcggcg tgttcggcag ctcggaccgc
     1021 acctggtcgg cgtccgccga cgccagcctg cgcgtgtcgt cgcacttcct ggtctcggcg
     1081 ggctggcgca cgctgaccat ggaccgcgcg caggtcagcc tctcgatgca agggccgcgc
     1141 gccgccgtgc agctggtgtt ctgacgcggg cctcgtcacc agggcgaccg gtcgcgcggc
     1201 cttctagcga cccggctcga tcgtggcacg ctggatgtag tcgagatcgg gaaagtcgcg
     1261 gcgcagatag gcattgccac cctcgaacac cggatcttgc ttccccgccc ggatcccgcc
     1321 gccggcccgc tcgccgtacc cggcgtagag cgcgtcggcg acgtccatgc cctcgatcac
     1381 gcgcgcgaac ggcacgaacg gctcggcgtc gaacgcggcg tggttgtcgc gcaggttgat
     1441 gaacacctgc gtcgtccggc cgtccggatc cttgaacgca taggccagcg ttccgcgcgt
     1501 gttggacagc acgcgcggat cgtcggggat cgtgcgggtg cgccagcgcc gggcgatcgc
     1561 cggctcgccg tggatgccga actgcgcgaa cactccggca cggacccgga agatcgccgc
     1621 gtcatcgtag tacccggcgc gcacgagtgc gtggaaccga tcgacgccgt gcggcgccca
     1681 ggcgcgctgg acctcgagga cgatcggccc ccgggtggtg tcgagccgga cgcgaaacgc
     1741 ctccggcgcg cgctgctgga gctcggcgct gccgggttgc agcaggcggt cgtgcccgcc
     1801 gcggtccgca cagcccgcgg ccaggacggc cgcgatcacg agcggaaggc catgcacggt
     1861 ggggttcatg acagcgccgg cgacggtctc gggcggtagc gatagtcgac cgacacgcgc
     1921 agccgatcgg ccgtgacgtt gtcgagcgcg cagtggaccg tgagcgcgga gaacaccacg
     1981 acgtcgccgg cctcgagatc gctcgcggcc cacgtcgcgt cgtcgggaac ggtggtgccg
     2041 acgacgccac cgcccgcgac gaccggcgcg tgcgggcgaa gcccggagcg gtgcgag
//
LOCUS       DASVOR010000779         1730 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387282,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000779 DASVOR010000000
VERSION     DASVOR010000779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1730)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1730)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1730
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387282"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1432)
                     /locus_tag="VF516_15355"
     CDS             complement(38..1432)
                     /locus_tag="VF516_15355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109109.1"
                     /translation="MLGEDSRLKALLARLMRPFTKVHPEEAAMVVLMTGCAFALLTAY
                     YLLKIVREPLILSGGGAAVKLYARAGQGLLMVGFVHFYGQLARRVGRVKLLAIVFLFF
                     LSNLAVFAVLAGTELSTGLAFGLAFFLWVGVFSYTVIAQFWALAADIYTEEQGKRLFP
                     IIGGGSSIGAMVGALFARWLMQFGVQALMTTALVILLGCLGVIVWIERRAATEHVPGP
                     HEDKHPDEPVARESAWHLLARDRYLLSIAGLVVFLNWVNSSGEYLLDRVLTAGASDAA
                     AAAGIDRLRYIGMFKADYSFWYNAIGVVLQLFAVARVFKLIGVRNALFVMPIFAFGAY
                     GLASLLPMLAVIRLVKIGENSLQYSLQDTTRNALFLVTSRVEKFVGKTAVDTIAVRLG
                     AVMTSITVLIGTSLGWSIATFLVINAGLALAWLGFVLLIRGEHRRRSTEEPAEIATEP
                     HSPGPGHHAAAAAR"
     gene            complement(1531..>1730)
                     /locus_tag="VF516_15360"
     CDS             complement(1531..>1730)
                     /locus_tag="VF516_15360"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8109110.1"
                     /translation="IITHPADATVVLDSRRLGKTPLDETIAAEPGKHVIRLRRKGYAL
                     HKLDVSLDADITEDISLTPQK"
BASE COUNT          305 a          611 c          576 g          238 t
ORIGIN      
        1 ctggcccagc accgctgagc gccgcggcgc ctcggaccta gcgcgcggcg gccgcggcgt
       61 ggtgcccggg ccccgggctg tgcggctcgg tcgcgatctc ggccggctcc tcggtgctgc
      121 gccgccggtg ctcaccgcgg atcagcagca cgaacccgag ccaggccagc gccagcccgg
      181 cgttgatcac gaggaacgtg gcaatcgacc agcccagcga cgtaccgatg aggaccgtga
      241 tcgaggtcat caccgcaccg agccgcaccg cgatggtgtc gaccgcggtc ttcccgacga
      301 acttctcgac ccgcgacgtc accaggaaca gcgcgttccg ggtcgtgtcc tgcagcgagt
      361 actgcagcga gttctctccg atcttgacca gccggatcac cgcgagcatc ggcagcaagc
      421 tggcgaggcc gtaggcgccg aacgcgaaga tcggcatcac gaacagcgcg ttgcgcacgc
      481 cgatcagctt gaacacacgc gcgactgcga acagctgcag cacgacaccg atcgcgttgt
      541 accagaacga gtagtcggcc ttgaacatgc cgatgtagcg cagccggtcg atgcccgcgg
      601 cggctgcggc atcgctcgcg ccggcggtga gcacccgatc caggaggtac tctccggagc
      661 tgttgaccca gttgaggaac acgacgaggc cggcgatcga caggaggtag cgatcacgcg
      721 ccagcaggtg ccaggcgctc tccctggcga ccggctcgtc ggggtgcttg tcctcgtgcg
      781 ggccagggac atgctcggtg gcggcgcggc gctcgatcca gacgatgacg ccgaggcaac
      841 cgagcaggat cacgagcgcc gtggtcatca gcgcctgcac gccgaactgc atcaaccacc
      901 gggcgaacag cgctccgacc atcgcaccga tcgagctgcc gccgccgatg atcggaaaca
      961 gccgcttgcc ctgttcctcg gtgtagatgt cggcagccag cgcccagaac tgcgcgatca
     1021 ccgtgtagct gaacacgccg acccacagga agaacgccag gccgaacgcc aggccggtcg
     1081 acagctcggt cccggcgagc acggcgaaca ccgcgaggtt cgagaggaag aacaggaaca
     1141 cgatcgcgag cagcttgact cgcccgacgc gccgcgcgag ctggccgtaa aagtggacga
     1201 accctaccat cagcagcccc tgcccggcgc gcgcgtacag cttcaccgcc gcgccgcccc
     1261 cgctcaggat gaggggctcc cgcacgatct tgagcaggta atacgccgtg agtagcgcga
     1321 acgcacaccc ggtcatcagc accaccatcg ccgcttcctc ggggtggacc ttggtgaacg
     1381 gccgcatcag gcgcgcgagc aacgccttga ggcgcgaatc ttcgcccagc atgcccgggg
     1441 tagagcaagc cgcgtgcaca gccccgagcc ccggatccgc gcacggcttg cgcggaacgc
     1501 cggcgccgcg ccagccttgc gggctgcgtc ctacttctgc ggggtcagcg agatgtcctc
     1561 ggtgatgtcg gcatcgagcg agacgtcgag cttgtgcagc gcgtagccct tgcggcgcag
     1621 cctgatcacg tgcttgcccg gctcggcggc gatggtctcg tcgagcggcg tcttgccgag
     1681 ccgcctgctg tcgagcacca ccgtcgcgtc cgccgggtga gtgatgatcc
//
LOCUS       DASVOR010000780        21255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_358318,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000780 DASVOR010000000
VERSION     DASVOR010000780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 21255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21255
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358318"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1752)
                     /locus_tag="VF516_15365"
     CDS             complement(<1..1752)
                     /locus_tag="VF516_15365"
                     /inference="COORDINATES: protein motif:HMM:NF003619.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HEX8109111.1"
                     /translation="MSTEVGTEAISREPSRTGAGERAPGDQAKRDEELARIDAFWRAA
                     NYLSLGQIYLLDNPLLRKPLAREHIKPRLLGHWGTTPGLNFLYAHLNRVIKTYDLDMI
                     YVIGPGHGGPALVANAYLEGTYSEVYPDIGQDEDGMRKLFKQFSFPGGIPSHVAPETP
                     GSIHEGGELGYALSHAYGAAFDNRDLLVACVVGDGEAETGPLATSWHGNKFLNPARDG
                     CVLPILHLNGYKIANPSFLARIPKDELGKLLEGYGYRPYFVEGHEPQAMHRQMAETLD
                     VIIQEIHRIWKDARERGRIDRPTWPMIVLRSPKGWTCPREIDGKRCEGTWRSHQVPMS
                     DMDKPEHVAVLERWMKSYHPEQLFDATGRLKPELAELAPRGQRRMSANPHANGGLLLR
                     DLRLPSFRAYAVEVVAPGAVLAEATRLQGKFLRDVMKLNLEHRNLRLFSPDENNSNRW
                     QDVLEVTNRCFMAQILPEDDHLAPDGRVMEVLSEHQCQGWLEGYLLTGRHGFFSCYEA
                     FIHIIDSMFNQHAKWLKVSRDIPWRRPIASLNYLLSSHVWRQDHNGFSHQDPGFIDHV
                     VNKKAEVIRVYLPPDGNT"
     gene            2107..3054
                     /locus_tag="VF516_15370"
     CDS             2107..3054
                     /locus_tag="VF516_15370"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8109112.1"
                     /translation="MVVAAVVVIAAAAVAWALWPRTSRPWYIIAGNGRLEATEYDVAT
                     KLAGRLAELAPHEGDAVQSGQRVGRIAIEDVRADWSAAKSQAQAADHASREARDSVRA
                     AESSLVLARQNLARVEYMYKQRATTADVLDRNRSDLRTAEANLAAARDRVAEAVSKTS
                     AAQSDAVSLGSRVSDAELKAPVTGPVLHRIVEPGAMLAVGAKVLTVLDLSDMWMNVYF
                     PTDEVGKISVGDEAKIVLDALPDEQIPARVTFVSPKNQFTPREVETHNEREKLMFRVK
                     VRVDQSWLANHGAQLKAGMPGVTYLRLDPTQPWPQRLKG"
     gene            3059..5791
                     /gene="rbbA"
                     /locus_tag="VF516_15375"
     CDS             3059..5791
                     /gene="rbbA"
                     /locus_tag="VF516_15375"
                     /inference="COORDINATES: protein motif:HMM:NF033858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HEX8109113.1"
                     /translation="MGSDRLNETVAARLVGVSHTYGKVHALDGIDLEIPANRMVGFIG
                     PDGVGKSSVLALLSGARRIQAGSVTVLDHDLKSSRARSAVQPRIAYMPQGLGRNLYPT
                     LSVQENVDFFGRVFGQDREERRRRIDDLLASTGLAAFRDRPAAKLSGGMKQKLGLCCS
                     LIHDPDLLILDEPTTGVDPLSRRQFWELIARIRAGRAGMTTLVATAYMEEADSFDWLV
                     AMDGGHVIGTGSPAELKARTHASTLEGAFVALLPEKGGGGPLVVPPRPAGHDVVINAE
                     GLTQRFGKFTAVDHVSFQIERGEIFGFLGSNGCGKTTTMKMLTGLLPPTEGEARLFGR
                     PVSGKDLATRRQVGYMSQAFSLYAELTVMQNLELHARLFDLEDREQRIAALVERFDLA
                     AHADITAEALPLGIRQRLSLAVAVVHSPRILILDEPTSGVDPVARDQFWALLIELSRQ
                     QGVTIFVSTHFMNEAERCDRISLMHAGKVLACDTPAALVASKGAANLESAFITYLTEV
                     AEPSAAPEPTAPAPAPAAQRSEAQPGSLFSPRRLLAYAYREGLELRRDRLRMTFALLG
                     SVLLMLFLGYGITLDVENLPFAVLDRDRTPESRDYVQNIAGSRYFQERPPLTDEADLR
                     HEMAAARIAVALEIPARFGTDFRGGRQPEVGTWIDGAMPFRGETARGYVEGMHLLYLS
                     DLREQTQGPQAGALPAVLETRYRYNQDVRSIIAMVPAVIPMLLMFIPALLAALAVVRE
                     KEMGSITNLYVTPVTRLEFLLGKQLPYVAVGMMSLLLLVIEARVVFGVPVKGSLLALL
                     LATFLYVGATTGLGFFISTLTRSQVAALAATSIITLLPTVSFSGVITPVSSLVGSSAL
                     IGQGWPATYFIRASRGIFTKALGLADLRPEMLALLIFPPVLMAAAVALLSRQEK"
     gene            5794..6915
                     /locus_tag="VF516_15380"
     CDS             5794..6915
                     /locus_tag="VF516_15380"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8109114.1"
                     /translation="MRPANVYHLGIKELRSLAADPILVFFVVVAFSFTVYANSTISFE
                     LRNAQIAIVDQDHSQLTQRIANAFLPPYFRAPLQVGWADMDGLLDHSQASFALVFPPS
                     FEQDVLEGRRPEVQLNVDANVVSQAFIGTGYIQKILYREVAEFLRTAPPGGELPIRLT
                     QRVSFNQNLQSSWFSGAMELVNSITMISILLVGAAVLREREHGTLEHLLVMPLTPAEI
                     MLAKVWSNGLVIVALAVLSLQIVIEGIIGLPMAGSLALFIAALVLFLFSTTAIGIYLS
                     TLARSMPQFALLVILTIIPLELLSGNLTPRESMPRFVQDIMLVAPTTHFVRIAQGILF
                     RGAGLSVLWPSMLAIVAIGAVLFLLALQRFRISVTQTQL"
     gene            6972..8354
                     /locus_tag="VF516_15385"
     CDS             6972..8354
                     /locus_tag="VF516_15385"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8109115.1"
                     /translation="MAIALYTGRASANERMTVDEAVKLALAQNLPLLATEEVASASHE
                     VVKSVRATLLPTVQAADQFQHWNCPGAISLVRLPEGLRCRSDLEGGPPPPPDLSAFTP
                     AQQQQLESLINGFAAIPVVVRDRTTNIFAVTIDQPLLGLWRRSVEVATARAAASAADA
                     GVLVTQADVAQGVRTYFLQLFEARAEEQIAAASVRELDEQVASAKTRLAVHVITESDL
                     LRVEVAAANARQDQISARADAEVRKAELLDVLGLAPSADVEFVEPTALLADSQPLAPD
                     VKAVTERAIARRPEVARATSEHEAAEHLTTARHLALLPEIDAQLGYIRTDGTIASIHN
                     QEYIGVSASWTVWDWGTRTHLADAAARVAHAAALELDERRRQVAVEVKTTLVQTNAAR
                     AAVAVAKQTIGSAEEAYHAMQARVTAGTATTTDLLDAQTALTRARLNLTRARYSEAVR
                     HVAYLRAIGE"
     gene            complement(8672..9763)
                     /locus_tag="VF516_15390"
     CDS             complement(8672..9763)
                     /locus_tag="VF516_15390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109116.1"
                     /translation="MDLSNNFFSPQGSNGRYCGTCHAPEDGWSITPATVALMFLLTDG
                     TDPLFVNNLDTDTPTSDMSTPAARWNSITMLRQGKFTRKVSPPVVRDYEVIAADDPFG
                     VGTTSSLWFFRRPLPAANFRSATVMWDGANTVATGLRDGLIKQARSNVTGAQQGAPAA
                     DAVIFDIVDYENALSFAQLVIPSVGRLDSDGAHGGPEAASAQPLVEGRFDLYDAWQSS
                     SNPRRRQIWRGQEIFNNVNPPSGHRCGGCHTAANNGQNVNGQMFDVGISRPALARPDM
                     AVYTFQRVSDGAIVQTTDPGRGIRTGNFADLNHFKVPGLRGLAARAPYFHNGLAATLE
                     DVVALYQKSLGFVFTPEEAADLVAFLQAL"
     gene            10650..10907
                     /locus_tag="VF516_15395"
     CDS             10650..10907
                     /locus_tag="VF516_15395"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HEX8109117.1"
                     /translation="MADDPVHPPAERTDHSPWNLLLLLSIVVPLLTFLYDGDEPRLFG
                     FPRFYWMQLSFVVLGIVCTAIVYRKTRRKPGLPAQNKERQP"
     gene            10904..12514
                     /locus_tag="VF516_15400"
     CDS             10904..12514
                     /locus_tag="VF516_15400"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX8109118.1"
                     /translation="MTGINWTALIVLAILFLIVAGMGFVAGSWRRPATMHHLDEWGLG
                     GRNFGGWITWFLIGGDIYTAYTFVAVPALLFATGATGFFAMPYTVILYPIAFLPLIRL
                     WSVSHRHGFVTPADFVRARFGSPTLALLVAITGIVATMPYIALQLVGIEAVLKTIGLT
                     GDDFVTKHLPLIIAFGILAAYTYQSGLRAPALIAFVKDTLIYVVILVAVLWLPHALGG
                     WAAIFDAANQKFTAAKRGSLLLPPGSQLQYLTLAFGSALALFLYPHSVTGILASKNRD
                     IIKRNMAALPAYTLLLGLIALLGFMAIAAAPQGVAPIVTDGKPDTNTVIPVLFGKMFP
                     SWFAGIAYAAIGIGALVPAAIMSIAAANLFTRNIYVEYLDQNSSHERQAQVSKVVSLV
                     VKFGALLVILFIDPQFSIDLQLIGGVIILQTLPAVAIGLFTRWFHRGGLIAGWFAGML
                     AGMGMLYSIPNPVTHREHFGGSAFALAKLGLGTNLTIYTGFVAVTVNLIVTILVTLIL
                     RAAGVADGRDATERSDYFADEGDPKLTR"
     gene            complement(12522..13430)
                     /locus_tag="VF516_15405"
     CDS             complement(12522..13430)
                     /locus_tag="VF516_15405"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8109119.1"
                     /translation="MTASDSTAANVTPVGFVGIGAMGEPMALNLVRAGTKVLAWNRTR
                     DKTAALAAAGATIAADVAEVFAQAPVVILMLLDDKAIDDVVGRGTPTFANRVKNRLII
                     HMGTTAPAFSKALDADIRAAGGRYVEAPVSGSRRPAELGQLVVMLAGEASAIEVARPL
                     LAPMCRDAIVCGPAPNALLMKLAVNQFMITMVTGLAEAVHFASSHKLDLDRLVSVINA
                     GPMASDISKIKISKLVARDFAVQASITNVLESNRLITGAARSAGIASPLIDVCYALYG
                     ETMRLGLGGSDIAAVLRAIEERSAKH"
     gene            complement(13504..14196)
                     /locus_tag="VF516_15410"
     CDS             complement(13504..14196)
                     /locus_tag="VF516_15410"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8109120.1"
                     /translation="MSTFWELADATRVQTEYIADRRLKWMQAAPLPSLQRVLSQYRFF
                     TIYYISDLALLVHRLPFSRIRSLLAEFLNDELGNSDAELSHPQLYDSFLLSIGVERSS
                     FENCAYRDNVALLEEISTLMLTESPEQGVGLRGLGGECLCQLYLEAMHGYFSKNPAIQ
                     AMADRIDWRFWNIHAGELDISHRVRLREAIDQAIATSPRVEREICAGYEKSKGAWEAF
                     WSNIFAREAVTA"
     gene            complement(14271..15482)
                     /locus_tag="VF516_15415"
     CDS             complement(14271..15482)
                     /locus_tag="VF516_15415"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8109121.1"
                     /translation="MDRYIFPEHVISCLAFDARDLFVVEIAPNPTRGTHRSRESKMEA
                     LDQVTRPSAHPPEPSVVADQLAAGSANHPVWKAQILRCISANWLYRDDMRFVLRIFSA
                     LEGTRRDCMSVLETRAGSNTGVRSISDSKPNPILSAWASELSETMDPTVELPTFYRYF
                     ALQCSLFCRDGDIGDVAAFLLGGELVMARAYTYMAKALREATDRPAPEVEPGKTRELA
                     GVIASAPSPAGPSSHQAALEQALAYYVQLFEDLYQAVRSRRMATASDRIQARASLQRE
                     DQPPIPLSPGVGPIIMETRDDDNNIHFSVERYPGNYEALDPRVVRIPPGRTNNRHKHA
                     HETLFYFISGTGRILVGSTWVPVKAGDAVFAPRWAIHQTHNTGNTELVLLAITDYYLT
                     HQVYVGAYDKV"
     gene            15923..16915
                     /locus_tag="VF516_15420"
     CDS             15923..16915
                     /locus_tag="VF516_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8109122.1"
                     /translation="MTTGTSTELVGAPVVSAAKPAEAVNPGARNTMIGVFFGLSTAVV
                     WAGWAVATRFAMKTTLAPHDVTFLRYSVSCLVLLPVLIKNGFALKQIGWVRLIVMVVG
                     AGAPFMLLTSTGMRFAPASHVATLMIGAMPLFVALMAAVLFRQRFNRMQVLGFATVLA
                     GVACIGGYAIVFDRSAGGEWRGDALFVLCGILFAGYTHAQRRSGITALHAASLVSTCS
                     FVAFSPFYFFVFHSNLATAPRQEVVFQTFAQGIGIAVLGLLLYGEAVRRLGAARGAVA
                     GSLVPGLASLFAAPLLGEFPRASTVVGVVLVSVGVLLVTGVLSRRTAEPAAAGR"
     gene            complement(16956..17294)
                     /locus_tag="VF516_15425"
     CDS             complement(16956..17294)
                     /locus_tag="VF516_15425"
                     /inference="COORDINATES: protein motif:HMM:NF022731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3088 family protein"
                     /protein_id="HEX8109123.1"
                     /translation="MQDQLFVLEPGFDDHGTSYFCPHCAQVVGFLTYFPQVRDTLEIS
                     AVDFPRPRQRIIELVGEANQAMPLLVLAGVPAAVPGVTVDEANGRRFVKNAISIMRYL
                     AVTRGVPVPH"
     gene            complement(17381..18115)
                     /locus_tag="VF516_15430"
     CDS             complement(17381..18115)
                     /locus_tag="VF516_15430"
                     /inference="COORDINATES: protein motif:HMM:NF015437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8109124.1"
                     /translation="MSLGHLIAEFEAADSLEQLQAAMNRAISALEIPAYVFVHRNEQS
                     REHVILAGVGGQAVDPQSSTSAWFDVVVARAADENLPFFWVTADLPCASDKNLDRVLH
                     DPLHGRDVDLEVSYGWTVPVHDAAGRTAVMSFIGSRDLDRFKLTIELHRATLHAMTIY
                     FQVYVCRMTRLQASVPAFELTPRERQCLEWAARGKSRSDIGQIMGLSPRTVKFHLENA
                     QRKLNVARTTQAVLRAVSLGLIAVTE"
     gene            18803..19375
                     /locus_tag="VF516_15435"
     CDS             18803..19375
                     /locus_tag="VF516_15435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109125.1"
                     /translation="MITCTTDPGTGAILRCEPGGGSGAGSGSGSGSNTCQDVDEDGDG
                     EPHDQGDDGDHTGTVSADHDGGGDASKDSDGDGISDDRDCDQRPGEDGMRTELPYDVR
                     PQLGATTRPVLDAFAERGGTAPQIVSVTMDGGTWRLTELQAGTAFTVTQADCDHAGNR
                     ATGRDRVVVTWKNADQTQSTDHLDVRYCER"
     gene            complement(19391..20290)
                     /locus_tag="VF516_15440"
     CDS             complement(19391..20290)
                     /locus_tag="VF516_15440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8109126.1"
                     /translation="MLYPGERAARAPISVLIVGETGTGKEVVAESIHRMSPRAGAPFV
                     RLNCAALTETLVESELFGHEKSAFTGATATKHGFFEAAAGGTLFLDEIGELPLTTQVK
                     LLRVLERWRIVRVGGTTELPVDVRLVCATHRDLEIEVQRGRFREDLFFRIAAFVIPVP
                     PLRDRRSEIAPLAVHFAHEIATDLRQPAVGFTPDALDALQHASWPGNVRELRNAIERA
                     VVLSEGGKIGREHLPEQILEQTRTPPRDALDVRQRVASVEREAVIAALDASDGNQTQA
                     AKRLGVSRFALIRMMAKHDIKRR"
     gene            complement(20401..>21255)
                     /locus_tag="VF516_15445"
     CDS             complement(20401..>21255)
                     /locus_tag="VF516_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8109127.1"
                     /translation="LNAVGPLTDHTAQGGSADDAFHVVIPSLPGYGFSAKPKNTGWDP
                     DHIARAWAELMKRLGYREFAAQGGDWGAIVTDLMGVQAPEGLLGIHSNMPGAVPPEIS
                     KAVPSGAPPPDGLSDDEKRAYEDLSFVYTKGIGYATELSLRPQTLYGLADSPVALAAW
                     MIDHDSRSYEDIAQAFVDGEPVDNLTPDELLDNITLTWLTNTGVSSGRLYWENKLGFF
                     DVKNVSVPAAVSVFPMEMYRAPRSWVEKAYPNLIYFNEVDRGNHFAAWQEPELYSNEV
                     RAGLRALR"
BASE COUNT         3300 a         7173 c         7144 g         3638 t
ORIGIN      
        1 cgtgttcccg tcgggcggca ggtagacccg gatcacctcg gccttcttgt tgaccacgtg
       61 gtcgatgaat ccgggatcct ggtggctgaa gccgttgtgg tcctgccgcc agacgtgcga
      121 gctcagcagg tagttgagcg aagcgatcgg ccgccgccac gggatgtctc tggacacctt
      181 caaccacttg gcgtgctggt tgaacatcga atcgatgatg tggatgaatg cctcgtagca
      241 gctgaagaag ccgtgccggc cagtgagcag gtagccctcg agccagccct ggcactggtg
      301 ctctgacagc acctccatca cgcgaccgtc gggggccagg tgatcgtcct cgggcaggat
      361 ctgcgccatg aagcagcggt tggtcacctc gaggacgtcc tgccagcggt tcgagttgtt
      421 ctcgtccggg ctgaacagcc ggaggttccg gtgttcgagg ttgagcttca tcacgtcgcg
      481 aaggaacttg ccctgcagcc gggtcgcctc ggcgagcacc gcgcccggcg ccacgacctc
      541 gacggcgtag gcgcgaaagc tcggcaagcg caggtcgcgc agcagcaggc cgccgttggc
      601 gtgcgggttc gcgctcatcc gccgctgccc tcggggcgcg agctccgcca gctcgggctt
      661 gagccggccg gtcgcgtcga acagctgttc ggggtgatag ctcttcatcc agcgctcgag
      721 caccgcgacg tgctccggct tgtccatgtc gctcatcggg acctggtgac tgcgccaggt
      781 gccctcgcag cgcttgccgt cgatctcgcg cggacacgtc caccccttgg gggagcgcag
      841 cacgatcatc ggccaggtcg gccggtcgat ccggccgcgc tcgcgcgcat ccttccagat
      901 ccggtggatc tcctggatga tcacgtcgag cgtctcggcc atctgccggt gcattgcctg
      961 gggctcgtgg ccctcgacga agtacggccg gtagccgtag ccctcgagca gcttcccgag
     1021 ctcatccttg gggatccgcg cgaggaagct cggattggcg atcttgtagc cattcaggtg
     1081 gaggatgggg agcacgcagc cgtcacgcgc cgggttcaag aacttgttgc cgtgccagct
     1141 cgtggcgagc ggtccggtct cggcctcgcc atcgccgacc acgcacgcga cgagcaggtc
     1201 gcgattatcg aacgccgcgc cgtacgcatg cgacagcgca tatccgagct cgccgccctc
     1261 gtggatcgat ccgggagtct cgggcgcgac gtggctgggg atcccgcccg ggaagctgaa
     1321 ctgcttgaac agcttccgca tcccgtcctc gtcctggccg atgtccggat agacctcgct
     1381 gtaggtcccc tcgagatacg cattggcgac cagcgccggg ccgccgtgcc ccgggccgat
     1441 gacgtagatc atgtcgaggt cgtaggtctt gatcacgcgg ttcaggtgcg cgtagaggaa
     1501 gttcaggccc ggcgtggtgc cccagtgccc cagcaagcgc ggcttgatgt gctcgcgggc
     1561 gagcggtttg cgcagcagcg gattgtcgag cagatagatc tggccgaggg aaaggtagtt
     1621 cgccgcgcgc cagaatgcgt cgatcctggc cagctcctcg tcgcgcttag cctgatcacc
     1681 gggggcgcgt tcgcccgcgc cggtgcggga tggttctctg gagatcgctt cggtgcccac
     1741 ttcggtgctc acttcggggt tcattctgat cgctcctcgc ccgatgcaac aaatgtgttc
     1801 ggcgctcctg gagcaggtag caaggagcga gccgcgacga cgaaatcgcg tcgttcctga
     1861 tcgcacggcg cgcaccgagc tcactgtcct ggccatcgcc catcgcgatc cggagcggat
     1921 gaccgacgac gccagcaagc ggtggcccct tcgcgtgcct gctggctctg tcgtcgacgc
     1981 acgccgcgcc tccaggcgcg gcagggcagc aatcaaccgc aggacaacgc gacagtgtct
     2041 gggcacatcg gttgcaccac ggagccgcgc gtggagcaaa cctttcgtac gaagaagtgg
     2101 tttcgcgtgg tggtcgccgc ggtcgtggtc atcgcggcgg cggccgtggc ctgggcgctc
     2161 tggcccagaa catcgcggcc ctggtacatc atcgccggta atgggcggct cgaggcaacg
     2221 gagtacgacg tggcgaccaa gctcgccggc cgtctcgccg agctcgctcc tcacgagggc
     2281 gacgcggtgc agtccggaca gcgggtggga cggatcgcca tcgaggacgt gcgcgcggac
     2341 tggtcggctg ccaagtcgca ggcgcaagca gcggatcatg cctcgcgcga ggcgcgcgac
     2401 agcgtgcgcg ctgcggagag ctccctggtg cttgcccgcc agaacctcgc ccgcgtcgag
     2461 tacatgtaca agcaacgcgc gaccacggcg gacgtgctcg atcgcaatcg atcggacctc
     2521 cggaccgccg aggccaacct ggcggccgcc cgagaccgcg tggccgaggc ggtttccaag
     2581 acctcggccg cccagtccga tgccgtgagc ctcggctcgc gcgtgtcgga tgccgagctc
     2641 aaggcgccgg tgacggggcc cgtgctccac cggatcgtcg aaccgggcgc catgctggcg
     2701 gtgggcgcca aggtcctgac cgtgctcgac ctctccgaca tgtggatgaa tgtctacttt
     2761 ccgaccgacg aggtcgggaa gatctccgtc ggcgacgagg cgaagatcgt cctcgacgcg
     2821 cttccggacg agcagattcc ggcgcgggtg accttcgtgt caccgaagaa ccaattcacg
     2881 ccgcgcgagg tcgagaccca caacgagcgg gagaagctga tgttccgcgt gaaggtccgt
     2941 gtcgaccaga gctggctcgc gaaccacggg gcccagctca aggccgggat gccgggggtg
     3001 acctacctcc gcctcgaccc gacgcagccc tggccccagc gcttgaaggg gtgaccgcat
     3061 ggggagcgac cgcttgaacg agaccgtcgc cgcccgcctc gtcggggtga gccacaccta
     3121 cggcaaggtg cacgcgctcg acggcatcga cctcgagatc cctgccaacc gcatggtggg
     3181 gttcatcggc cccgacggcg tcggcaagtc cagcgtgctc gccctgctct cgggggcgcg
     3241 tcgcatccag gccggcagcg tcaccgtgct tgatcacgat ctcaagagct cccgtgcacg
     3301 atccgcggtg cagccgcgca tcgcgtacat gccgcagggg ctcggccgca atctctaccc
     3361 gacgctgtcg gtccaggaga acgtcgactt cttcgggcgt gtattcggcc aggaccggga
     3421 ggagcggcgc cgccggatcg acgatctgct cgccagcacg gggctcgccg cgttccgcga
     3481 ccgccccgcc gcgaagctgt ccggcggcat gaagcagaag ctcggcctgt gctgctcgct
     3541 gatccacgac ccggacctcc tgatcctcga cgagccgacg accggcgtcg atccactgtc
     3601 ccgccgccag ttctgggagc tcatcgcgcg gatccgcgcg ggccgtgcgg ggatgaccac
     3661 gctcgtcgcc acggcgtaca tggaggaggc ggacagcttc gactggctgg tcgcgatgga
     3721 cggcgggcac gtgatcggca cgggcagccc cgccgagctg aaggcgcgca cccacgcgtc
     3781 taccctcgaa ggcgccttcg tggcgctgtt gccggagaag ggcggcggtg gccccctggt
     3841 cgtcccgccg cgccccgccg ggcacgacgt ggtcatcaac gccgaaggcc tcacccagcg
     3901 gttcggcaag ttcaccgccg tcgatcacgt gagcttccag atcgagcggg gggagatctt
     3961 cggcttcctc gggtccaacg gctgcggcaa gacgaccacg atgaagatgc tcacgggcct
     4021 gttgccaccc accgagggcg aggcccggct gttcggccgc ccggtgagcg gcaaggatct
     4081 cgcgacccgc cgtcaggtcg gctacatgtc gcaggcgttc tcgctctacg ccgagctgac
     4141 ggtcatgcag aacctcgagc tgcacgcgcg gctgttcgat ctcgaggatc gagagcaacg
     4201 gatcgccgcc ctggtcgagc gcttcgacct cgcagcgcat gccgacatca cggcggaggc
     4261 gctgccgctc gggatccgcc agcggctgtc gctcgccgtc gcggtggtcc actcgccgcg
     4321 catcctcatc ctggacgagc caacctccgg cgtcgatccc gtcgctcgcg accagttctg
     4381 ggcgctgctc atcgagctct cgcgacagca gggcgtcacc atcttcgtct cgacgcactt
     4441 catgaacgag gcggagcgct gcgatcggat ctcgttgatg cacgccggca aggtgctggc
     4501 gtgtgacacg ccagcggccc tggtggcctc gaagggcgcg gcgaacctcg agagtgcctt
     4561 catcacctac ctgacggagg tcgccgagcc gagcgccgcg ccggagccca ccgcgccggc
     4621 gcccgcaccg gccgcccagc gctccgaggc gcagcccggt tcgctgttca gcccgagacg
     4681 cctgctcgcc tatgcatatc gcgagggcct cgagctccgc cgcgatcgcc tccgcatgac
     4741 gttcgcgctg ctcggctccg tgctgctgat gctgttcctc ggctacggga tcacgctgga
     4801 cgtggagaac ctgccgttcg ccgtgctcga ccgcgaccgc acgccggagt cacgcgacta
     4861 cgtccagaac atcgccggca gccgctactt ccaagagcgt ccgccgctga ccgacgaggc
     4921 agacctgcgg cacgagatgg ccgccgcgcg catcgcggtc gcgctcgaga tcccggcgcg
     4981 cttcggcacc gactttcgcg gaggtcgcca gcccgaggtc ggcacatgga tcgacggggc
     5041 gatgccgttc cgcggggaga cggcgcgcgg ctacgtcgag ggcatgcacc tgctctacct
     5101 ctccgatctg cgcgagcaaa cacagggacc gcaggccggt gcgctccctg ccgtgctcga
     5161 gacccgatac cgctacaacc aggacgtccg cagcatcatc gcgatggtgc cggcggtgat
     5221 cccgatgctc ctcatgttca tcccggcgct gctcgcggcg ctcgccgtgg tccgggagaa
     5281 ggagatgggc tcgatcacca acctctacgt cacgccggtc acccgtctcg agttcctgct
     5341 cggcaagcag ctgccgtacg tcgccgtggg catgatgagt ctgctgttgc tcgtgatcga
     5401 ggccagggtc gtgttcggtg tgccggtcaa gggcagcctg ctcgcgctgc tcctggcaac
     5461 gttcctctac gtcggcgcga ccaccggcct gggcttcttc atctcgacgc tcacgcgtag
     5521 ccaggtcgcg gcgctcgccg ccacgtcgat catcacgctg ctcccgaccg tctcgttctc
     5581 gggtgtcatc accccggtgt cctcgctggt ggggagcagc gcgctcatcg gccagggctg
     5641 gcccgcgacg tacttcatcc gtgcgtcgcg cgggatcttc accaaggcgc tcggtctggc
     5701 ggacctgcga cccgagatgc tcgccctctt gatctttccc ccggtgctca tggcagcggc
     5761 cgtcgcgctg ctgtcgcgcc aggagaagtg acgatgcgac cggccaacgt gtaccacctc
     5821 gggatcaagg agctgcgcag cctcgccgcg gatcccatcc tcgtgttctt cgtcgtggtg
     5881 gcgttctcgt tcacggtgta cgccaactcg accatctcgt tcgagctgcg caacgcgcag
     5941 atcgcgatcg tcgatcagga tcactcgcag ctcacccagc ggatcgcgaa cgcgttcctc
     6001 ccgccgtact tccgcgcccc cctgcaggtc gggtgggcgg acatggacgg actgctcgat
     6061 cacagtcagg cgagcttcgc gctggtgttc cctcccagct tcgagcagga cgtgctcgag
     6121 gggcgccgcc cggaggtcca gctcaacgtc gacgccaacg tcgtctcgca ggcgttcatc
     6181 ggcaccggct acatccagaa gatcctctac cgcgaggtcg ccgagtttct ccggaccgcg
     6241 ccgcctggcg gcgagctccc gatccggctc acgcagcgcg tcagcttcaa ccagaacctc
     6301 cagagctcct ggttctcggg cgcgatggag ctcgtcaaca gcatcacgat gatctcgatc
     6361 ttgctcgtgg gcgcagccgt cctgcgcgag cgcgagcacg gaacactcga gcacctgctc
     6421 gtgatgccgc tcacgcccgc cgagatcatg ctcgcgaagg tctggtccaa cgggctggtc
     6481 atcgtcgcgc tcgccgtgct ctcgctccag atcgtgatcg aagggatcat cggccttccc
     6541 atggcgggat cgctcgcgct gttcatcgcg gcgctggtgc tgttcctgtt ctcgacgacc
     6601 gcgatcggga tctacctcag cacgctggct cgctcgatgc cgcagttcgc cttgctcgtc
     6661 atcctcacga tcattccgct cgagctcctg tccggtaacc tgaccccgcg cgagagcatg
     6721 cccaggttcg tccaggacat catgctcgtc gcgcccacga ctcacttcgt gcggatcgca
     6781 cagggcatcc tgtttcgcgg cgccggcttg agcgtcctgt ggcccagcat gctcgcgatc
     6841 gtcgcgatcg gcgccgtcct gttcttgctc gcgctgcagc ggttccggat ctcggtgacg
     6901 cagacccagc tgtgaacact acgttgggag aacgaatgat gcgaggacaa cgaatgatgc
     6961 gagcgctcgg attggcgatc gcgctgtata ccgggcgggc ctccgcgaac gaacgcatga
     7021 cggtggacga ggcggtgaag ctcgcgctcg cgcagaacct gccgctcctc gcgacggagg
     7081 aggtggcgag cgcgagccac gaggtcgtga agtcggtccg cgcgacgttg ctgccgacgg
     7141 tccaggccgc cgaccagttc cagcactgga actgtccggg ggccatctcg ctggtgcgcc
     7201 tcccggaggg tctccggtgc cgctccgacc tggagggcgg ccccccgccg ccgccggacc
     7261 tctcggcctt cacgccggcc cagcagcagc agctggaatc actcatcaac gggttcgccg
     7321 cgattccggt ggtcgtccgc gaccggacga ccaacatctt cgccgtgacg atcgaccagc
     7381 cgctcctggg gctctggcgc cggagcgtcg aggtcgccac cgcgcgcgcc gccgcgagcg
     7441 ccgcggacgc aggcgtcctg gtcacccagg cggacgtcgc gcagggcgtg cgcacctact
     7501 tcctgcagct gttcgaggcg cgtgccgagg agcagatcgc cgcggcatcg gtgcgcgagc
     7561 tggacgagca ggtcgcgtcc gcgaagacgc ggctcgccgt gcacgtgatc accgagtcgg
     7621 atctcctgcg tgtcgaggtc gccgccgcga atgcgcggca agaccagatc tctgcgcgcg
     7681 ccgatgccga ggtccgcaag gcggagctcc tcgacgtgct cggcctcgca ccgagcgccg
     7741 acgtggagtt cgtcgagccg acagcgctgc tcgctgactc ccagccgctg gcaccggacg
     7801 tgaaggccgt gacggagcgg gcgatcgcgc gccgtccgga ggtcgcccgg gcaacgtccg
     7861 agcacgaagc cgccgagcac ttgacaacgg cgcggcacct tgcgctgctc ccggagatcg
     7921 atgcccagct gggatacatt cgcaccgacg ggacgatcgc ctccatccac aaccaggagt
     7981 acatcggagt gtccgcgagc tggaccgtct gggactgggg tacgcgcacc catctggcgg
     8041 acgcggccgc gcgggtggcc catgcagccg ccctggagct cgacgaacgc cggcgacagg
     8101 tcgcggtcga ggtgaagacc acactggtgc agaccaacgc ggcacgcgcc gccgtggcgg
     8161 tcgcgaagca gaccatcggc agcgcggagg aggcctatca cgccatgcag gcgcgggtga
     8221 cggcgggcac cgcgacgacg accgatctcc tcgatgcgca gaccgcgctg acccgggcgc
     8281 ggctcaacct cacgcgcgcg cggtactcgg aggccgtccg gcacgtggcg tacctccgcg
     8341 cgatcggcga gtgacgcgcg aactggctcg cagccgagtc ccgcgcgtca gccgcctgct
     8401 gcacaagaac gagcgcgatg tgcacgaagc accgcgttgc cccaggggcc ctcgctccgg
     8461 accgggttgt catgtcgggt ccatcaccgc cagggccaaa tcgctggttg aagtcggcga
     8521 gcgcacgctc gctgtggtgc gcgacgcggt gctgattttc gatcggccga acccgtgcag
     8581 gctgcgccga tgcaggaggc agcgtgatgg cgtccggaac gacaagaacc cgccgaagcg
     8641 ggttcagtgt caagggattg ccaaatttcg gctacagcgc ctggaggaac gcgaccagat
     8701 cggcggcctc ctcgggcgtg aacacgaagc cgagcgactt ctggtagagg gcgacgacgt
     8761 cctcgagcgt ggccgcgagg ccgttgtgga agtacggcgc gcgcgcggcc aggccgcgca
     8821 ggccggggac cttgaagtga ttcagatcgg cgaagttgcc ggtgcggatg ccgcggcccg
     8881 gatcggtggt ctgcacgatc gcgccgtcgc tgacgcgctg gaacgtgtag accgccatgt
     8941 ccggccgggc gagcgcgggc cgcgatatgc cgacgtcgaa catctggccg ttcacgttct
     9001 ggccgttgtt ggcggcggtg tggcagccgc cgcagcggtg gccgctcggc gggttcacgt
     9061 tgttgaagat ctcctggccg cgccagatct ggcggcgccg cgggttgctg ctggactgcc
     9121 aggcgtcgta gaggtcgaac cggccctcga ccagcggctg ggcgctcgcc gcctccgggc
     9181 caccgtgcgc gccgtcggag tcgagccgcc cgaccgaggg aatgacgagc tgggcgaacg
     9241 acagcgcatt ctcgtagtcc acgatgtcga agatcacggc gtcggcggcg ggcgcgccct
     9301 gctgggcgcc ggtcacgttg ctgcgcgcct gcttgatcag gccgtcgcgc agcccggtcg
     9361 cgaccgtgtt ggcgccatcc cacatcacgg tggcgctgcg gaagttcgct gccggcagcg
     9421 ggcggcggaa gaaccacagg ctcgaggtcg tgccgacgcc gaacggatcg tcggccgcga
     9481 tgacctcgta gtcgcggacc accggcggcg acaccttgcg ggtgaacttg ccctgccgca
     9541 gcatcgtgat cgagttccag cgcgccgccg gagtcgacat gtcggatgtg ggagtatcgg
     9601 tgtcgaggtt gttgacgaac agcggatcgg tcccgtcggt gagcaggaac atcagcgcga
     9661 cggtcgccgg cgtgatgctc cagccgtcct cgggagcgtg gcatgtcccg cagtagcggc
     9721 cgttgctgcc ctgggggctg aagaagttgt tgctcagatc caccgagccg gaagggtgaa
     9781 acgacgcggc gaatcccgag ctatcaggaa ccgggagatt gttcgggatc tgatggaatt
     9841 gccgagcgag taattcctgc cgatctgggc tcgtatcctg gggcgcttgc gcgcatcccg
     9901 cgacgaggag aactgcagag atcgacgtgg acagggacgt gtaggtgaaa gaatgcacgt
     9961 gatgaccctc cgctgtcgtg gaccttagaa tgttcatgca gcgaaaagca cctatattca
    10021 tctggcagtc tgacgattgc tcttgtgcca tcacggacac cggcaatccg tcacattcat
    10081 gtacacctag aaaaaagcta tcgctcggtc tccgtcacgt gcctcgacgc acgaaaaatc
    10141 gtctgtcacg tttacgtaca ctgtcgaaaa aacgatcgct cggccgccgc catgtgcctc
    10201 gacgtacgaa aaacggtctg tcacgtttgc gtacactgtc gaaaaaacga tcgctcggcc
    10261 gccgtcacgt gcttcgcggc acagaaacaa tccgccctgc gcaaatgccg tcgaacaacg
    10321 atcgctcggc cgccgtcacg ttccttgatg gaaaaagcag ctccagagat tccagcattc
    10381 tgcgacaacg caattgatga gtcgtgatga ttacagtgcg aatgctgtcg ggtaatcgac
    10441 gccggtgagc gatatccggc gaccgcgtcg gtgtatgaca ggctcgtact cgaacgcgca
    10501 gatctgcacc gagtcggcca tcgcgacgcc gctccgccgt ctcaagctat cgagatcgct
    10561 acgataacgc accgagtcct ggattcgcgg cacgagcagg cgctgcacat ctccgacaaa
    10621 tcccactgga tccccgttac agtgcccgcg tggccgacga tcccgtacat ccacccgccg
    10681 agcgaaccga tcacagcccg tggaacctgc ttctgctgct gtcgatcgtg gttccgctct
    10741 tgaccttcct ctacgacggc gacgagccgc ggctgttcgg cttcccgcgg ttctactgga
    10801 tgcagctgtc gttcgtcgtg ctcggcatcg tgtgcaccgc gatcgtgtac cggaagacca
    10861 ggcgcaagcc cgggctgccc gcgcagaaca aggagcgcca gccgtgaccg ggatcaactg
    10921 gaccgcgctg atcgtcctcg ccatcttgtt cctgatcgtc gcgggcatgg gcttcgtcgc
    10981 cgggagctgg cggcggccgg cgacgatgca ccacctcgac gagtgggggc tcggcggccg
    11041 gaacttcggc ggctggatca cctggttcct gatcggcggc gacatataca ccgcgtacac
    11101 gttcgtcgcg gttcccgcgc tcttgttcgc gaccggcgcg accgggttct tcgcgatgcc
    11161 gtacaccgtg attctctacc cgatcgcgtt cctcccgctg atccggctgt ggtcggtgtc
    11221 gcatcgccac ggtttcgtga ccccggcgga cttcgtgcgc gcccggttcg gttcaccgac
    11281 gctggcgctg ctggtcgcga tcaccggcat cgtcgcgacc atgccctaca tcgcgctgca
    11341 gttggtcggc atcgaggcgg tgctcaagac gatcgggctc accggcgatg acttcgtcac
    11401 caagcacctg ccgctgatca tcgcgttcgg catcctcgcc gcgtacacct accagtccgg
    11461 gctgcgcgcg ccggcgctga tcgcgttcgt caaggacacg ctgatctacg tcgtgatcct
    11521 cgtggcggtg ctgtggctgc cgcacgcgct cggcggctgg gccgcgatct tcgacgccgc
    11581 gaaccagaaa ttcaccgcgg ccaagcgcgg cagcctgctg ttgccgccgg gcagccagct
    11641 ccagtacctg acgctggcgt tcgggtcggc gctcgcgttg ttcctgtatc cgcactcggt
    11701 gacaggcatc ctggcgagca agaaccgcga catcatcaag cgcaacatgg cggcgctgcc
    11761 cgcctacacg ctgctgctcg gcctgatcgc gctgctcggg ttcatggcga tcgccgccgc
    11821 cccgcagggc gtcgccccga tcgtcaccga tggcaagccc gacaccaaca ccgtgatccc
    11881 ggtgctgttc ggcaagatgt tcccgtcgtg gttcgccggc atcgcctatg ctgcgatcgg
    11941 gatcggcgcg ctggttcccg ccgcgatcat gtcgatcgct gcggccaacc tgttcacccg
    12001 caacatctac gtcgagtacc tggaccagaa cagctcgcac gagcggcagg cccaggtctc
    12061 caaggtcgta tcgctggtgg tcaagttcgg cgcgttgctc gtgatcctgt tcatcgaccc
    12121 gcagttctcg atcgacctcc agctgatcgg cggcgtgatc atcctgcaga ccctgccggc
    12181 ggtcgcgatc ggtctgttca cgcgctggtt ccatcgcggc ggcttgatcg ccggctggtt
    12241 cgcggggatg ctcgccggaa tggggatgct gtacagcatc cccaacccgg tgacccaccg
    12301 cgagcacttc ggcggctcgg cgttcgcact cgccaagctc ggcctgggaa ccaacctgac
    12361 catctacacc gggttcgtcg cggtgaccgt gaacctgata gtcacgatcc tcgtcacgct
    12421 gatcctccgc gccgcgggtg tcgccgacgg ccgcgacgcg accgagcgct ccgactactt
    12481 cgccgacgag ggcgatccca agctgacccg gtgagcgcag atcagtgctt cgcgctgcgc
    12541 tcctcgatcg cgcgcagcac cgcggcgatg tcggagccgc cgagcccgag ccgcatcgtc
    12601 tcgccgtaca gcgcgtagca cacgtcgatc agcggtgatg cgatgccggc actgcgcgcc
    12661 gctccggtga tcagccggtt gctctccagc acgttggtga tcgatgcctg gaccgcgaag
    12721 tcgcgggcga ccagcttcga gatcttgatc ttcgagatgt cgctcgccat cggtccggcg
    12781 ttgatcaccg agaccaggcg gtccaggtcg agcttgtggc tgctcgcaaa gtgcacggcc
    12841 tcggcgaggc cggtgaccat cgtgatcatg aactggttga ccgccagctt catcaggagc
    12901 gcgttcggcg ccggcccgca cacgatggcg tcgcggcaca tcggtgcaag cagcgggcgc
    12961 gccacctcga tcgcgctggc ctcgcctgcg agcatcacga ccagctggcc gagctcggcg
    13021 ggccggcgcg agcccgagac cggggcctcg acatagcgcc cgccggcggc ccggatatcg
    13081 gcatccagcg ctttcgagaa cgccggcgcg gtcgtcccca tgtggatgat cagccggttc
    13141 ttcacccggt tcgcgaacgt cggcgtgccg cgcccgacca cgtcgtcgat cgccttgtcg
    13201 tcgagcagca tgaggatgac gaccggggcc tgcgcgaaca cctcggcgac atcggccgcg
    13261 atggtcgcgc cggccgccgc cagcgccgcg gtcttgtccc gcgtgcggtt ccacgcgagg
    13321 accttggtcc ccgcccggac caggttgagc gccatgggtt cccccatggc cccgatcccg
    13381 acgaagccga ccggcgtcac gttcgcggca gtcgaatcac tggctgtcat tgcgcatcct
    13441 gttcttggtg gtggagaacg gtggaggtag gcgcgcggtg cgccgctcag ggagcggcac
    13501 ggttcatgcc gtgaccgcct cccgggcgaa gatgttactc cagaacgctt cccacgcacc
    13561 cttgctcttc tcgtacccgg cacagatctc gcgctccacc cgcggcgagg tcgcgatcgc
    13621 ctgatcgatc gcttcgcgca gccgcacgcg atgtgagatg tcgagctcgc cagcgtggat
    13681 gttccagaac cgccagtcga tccggtccgc catggcttgg atcgccggat tcttgctgaa
    13741 gtagccgtgc atcgcctcca ggtagagctg gcacaggcac tcaccaccca gcccgcgcag
    13801 cccgacaccc tgctcgggtg actcggtgag catcagtgtg ctgatctcct ccagcagcgc
    13861 gacgttgtcc cgataggcgc agttctcgaa cgacgaccgc tcgacgccga tgctgagcaa
    13921 gaagctgtcg tagagctggg ggtgcgacag ctcggcgtcc gaattgccga gctcgtcgtt
    13981 gaggaactcc gccagcaggc tgcgtatccg gctgaacggc aggcggtgga cgagcaacgc
    14041 caggtcgctg atgtagtaga tggtgaagaa gcggtactgc gagagcacgc gctgcaggct
    14101 cggcagcggc gcggcctgca tccacttgag gcgccggtcg gcgatgtatt cggtttggac
    14161 tcgggtggcg tccgcgagtt cccagaaggt gctcatcggg gacatcgttc cggacttggt
    14221 ctcggctgtt tcaggtgcca tggttgcaac tcgttaggca ggggttcggg tcagaccttg
    14281 tcgtaggctc cgacgtagac ctggtgggtg aggtagtaat cggtgatcgc gagcaggacg
    14341 agctcggtat tgccggtgtt gtgagtctgg tgaatggccc agcgcggcgc gaacacggcg
    14401 tcgccagcct tcaccggcac ccaggtcgat ccgacgagga ttcgaccggt accggagata
    14461 aaatagaaca gcgtctcgtg ggcgtgcttg tgtcggttgt tggtcctgcc cggggggatc
    14521 cgcacgaccc gcggatccag cgcctcgtag ttaccgggat agcgctccac cgagaaatgg
    14581 atgttgttgt cgtcgtcccg ggtctccatg atgatcggcc cgacgccggg cgacagcggg
    14641 atcggtggct gatcctcccg ctggaggctg gcccgggcct ggatgcgatc cgacgcggtg
    14701 gccatgcgcc gagaccgcac cgcctggtag agatcctcga acagctggac gtagtacgcc
    14761 agcgcctgct ccagcgcggc ctggtgcgat gacggaccgg cgggtgacgg tgcgctcgcg
    14821 atgacgccgg cgagctcgcg tgtcttgccg ggctcgacct cgggggccgg gcgatcggtg
    14881 gcctcgcgca gcgccttggc catgtacgta tatgcgcgtg ccatgaccag ctcgccgccg
    14941 agcaggaagg ccgccacgtc accgatgtcg ccgtcgcgac agaacaatga acactgtagt
    15001 gcaaaatacc ggtaaaaggt cggtagctcg accgtcgggt ccatcgtctc cgacagctca
    15061 ctcgcccacg cggagagaat cgggttcggc ttgctgtccg agatcgaccg tacgccggta
    15121 ttgctgccag cgcgggtctc gaggaccgac atgcagtctc gccgcgttcc ctcgagtgca
    15181 gagaagattc gcaggacgaa ccgcatgtcg tcccgataca gccagttcgc cgagatgcag
    15241 cgcaggatct gtgctttcca caccggatgg ttggctgagc ctgccgctag ctgatctgca
    15301 acgacggacg gctcgggcgg atgggccgac ggtcgagtca cctggtctag cgcttccatc
    15361 ttggattccc tgcttctgtg agtgccgcgg gttgggttcg gtgcaatttc aacgacaaac
    15421 agatcgcgcg catcgaacgc gaggcaggaa atcacatgtt ctggaaagat gtacctgtcc
    15481 atgaggacag gcacggagtc ctcgtgatgg gcggacatcg agtgaactgc cgaagattcc
    15541 tggattgtct attgatgtag acggttgtgc gtttgaccgc gtgtttgaca ctgtccatct
    15601 ggacgtcttg ccgtcgatcc ggatttgcat gcgcagcatt gcgcggcggc ggatcccgag
    15661 gtcgggctgg tactcggcgc gtcgcgtcgc gtcggcatcc cgcatccata gcggtgcgga
    15721 cctcggttcg agcggcgccg aagctgcgca gcattttgct gcagcgagcg agccgcccgc
    15781 gaaaccgacc ggcccggccg acgtggtgag cgcggttagg ataccatcct cgaatgaatc
    15841 cgcaaatgcg cgctgccaca ggtcgatccg caggaccggc cggacgaagc tcggcgatct
    15901 tgcacgccca caggtgcggg tgatgacgac ggggacgagc acggagctgg tcggggcccc
    15961 ggtggtatcc gccgcgaagc cggccgaggc cgtcaaccca ggtgcccgga acacgatgat
    16021 cggcgtgttc ttcgggctct cgacggccgt ggtgtgggcg ggatgggcgg tggcgacgcg
    16081 gttcgcgatg aagacgacgc tcgcgccgca tgacgtcacg ttcctgcgct actcggtgtc
    16141 ctgcctggtc ctgctgccgg tcctgatcaa gaacgggttt gcgctgaagc agatcggctg
    16201 ggtcaggctg atcgtgatgg tcgtcggcgc cggcgctccg ttcatgttgc tgacctcgac
    16261 cggcatgcgg ttcgcgccag catcgcatgt cgccacgctg atgatcggcg cgatgccgct
    16321 gttcgttgcc ctgatggccg ccgtcctgtt ccggcagcgg ttcaaccgga tgcaggtgct
    16381 cgggttcgcc acggtgctcg ccggggtggc gtgcatcggc ggctacgcga tcgtgttcga
    16441 ccgctcggcg ggtggggagt ggcgcggtga tgccctgttc gtgctgtgcg gcatcctgtt
    16501 cgccggctac acccacgcgc agcgccgctc cggcatcacc gccctgcatg ccgcatcgct
    16561 ggtgagcacc tgctcgttcg tcgcgttctc cccgttctat ttcttcgtgt tccactccaa
    16621 cctcgcgacc gcgccgcggc aggaggtggt gtttcagacc ttcgcgcagg gcatcggcat
    16681 cgcggtgctc ggcctgctgt tgtacggcga ggccgtgcgg cggctcggcg cggcgcgcgg
    16741 cgcggtggcc ggctcgctgg ttccgggcct ggcatcgctg ttcgcggcgc cgctgctcgg
    16801 cgagttcccg cgggcctcga ccgtggtcgg ggtcgtgctg gtgagtgtcg gcgtcttgct
    16861 cgtcaccggt gtgctgtcgc ggcgcacggc agagccggcg gctgccggac gctaggcgac
    16921 gctaggcgac gccgggcgtg gggcgtggca gccggtcagt gcgggaccgg gacgccgcgg
    16981 gtcacggcga ggtagcgcat gatcgagatc gcgttcttga cgaagcgacg cccgttggcc
    17041 tcgtcgaccg tcacgcccgg caccgccgcc ggcacgcccg cgaggaccag gagcggcatc
    17101 gcctggttcg cttcgcccac cagctcgatg atccgctggc gcggcctcgg gaagtccacc
    17161 gcgctgatct ccagcgtgtc gcgtacctgt gggaagtacg tcaggaaccc cacgacctgc
    17221 gcgcagtgag gacagaagta cgacgtcccg tgatcatcga agccaggctc cagcacgaac
    17281 agctggtctt gcataggcgt attctactgc tgttgccgac catccgtcca ccctgctcgg
    17341 cgtcgcgcgg gtcagcatgg gcgtcggccg gcctcgtcgg tcattcggtc acggcgatga
    17401 gaccgagcga gacggcgcga agcaccgcct gcgtggtgcg cgccacgttc agcttgcgct
    17461 gcgcgttctc gagatggaac ttgacggtgc gcgggctcag acccatgatc tggccgatat
    17521 ccgagcggct cttgccccgg gcggcccact ccaggcactg ccgctcgcgt ggcgtgagct
    17581 cgaacgccgg cacgctggcc tgcagtcttg tcatcctgca gacgtacacc tgaaagtaga
    17641 tcgtcatcgc atgcaacgtc gcccgatgca gctcgatcgt cagcttgaac cggtcgagat
    17701 cgcgcgatcc gatgaagctc atcactgcgg tgcggccggc cgcatcatgc accgggaccg
    17761 tccagccgta gctgacctcc aggtcgacgt cgcgaccgtg cagcgggtca tgcagcaccc
    17821 gatcgaggtt cttgtcggac gcacacggca gatccgcggt gacccaaaag aacgggaggt
    17881 tctcgtcggc ggctcgcgcc acgacgacgt cgaaccatgc cgacgtgctg gactgcgggt
    17941 cgacggcttg tcccccgacg cctgcgagga tcacgtgctc gcgtgactgc tcgtttcgat
    18001 gcacgaagac gtatgcgggg atctcgagcg cagagatcgc ccgattcatg gcagcttgca
    18061 gctgttccag gctgtcggcg gcttcgaact cggcgatgag gtggcccagg ctcacgacgc
    18121 gtctccggcc gaacagcgta ttgcacccgc ggtcgtctcc ctcgaagcac agatttcggt
    18181 cgtgacctgg aatacctcgc aaagagtgtt agaaatcgag cgcacgacat gtatgtcagt
    18241 gtgtcgctcc cgcgtcaggc cgtaagtgat tgccaggccc atgggccgga tacggcatat
    18301 cacgcgctta ctgcccattg tgggttcttt cttacacata cagccacggc gatcccatcg
    18361 agcacccgct cgtcccgtga agacggaatc gccgcccatg ggctctgaag tcgcgataca
    18421 atatttcgca gagtgcgtca aggcaactcc gttttcggac cttacaagac ggttcggtct
    18481 tgtcggacac acggtcgtcg atcgactccg gacgtcttcg ccgacgaggc gatccggaga
    18541 tccaccgatt gccgcggcgg gcattgccag ttgccggctc gcgtgcggcg accgcacgac
    18601 tcgctgtacg aagcctgctc gtcgccctcc gccggcgcgg ccaactcacg gatcgcgctg
    18661 gcgttctcgg tggcatgttg ctggcaatgg cggtctggcc atgcagcttc gatcgactct
    18721 ctcgctgacc ttgatggctg tcctcccgct cggcgccctc gcatccggtt gctcgtccga
    18781 gcacacatcg accgcaggcc agatgatcac ctgtaccacc gatcccggta ccggcgcgat
    18841 cctgcgctgc gagccgggcg gcggctccgg cgctggcagc ggcagcggta gcggctcgaa
    18901 cacctgccag gacgtcgacg aggacggcga cggcgagccc cacgaccagg gcgacgacgg
    18961 cgaccacacc ggcaccgtca gcgccgacca cgacggcggc ggggacgcca gcaaggacag
    19021 cgatggcgac ggcatctccg acgaccgcga ctgcgaccag cgccccggcg aggacggcat
    19081 gcggaccgag ctgccgtacg acgtgcggcc ccagctcggc gcgacgacgc gtccggtgct
    19141 cgacgcgttc gccgagcgcg gcgggacggc gccgcagatc gtctcggtca cgatggacgg
    19201 cggcacgtgg cggctcaccg agctccaggc cggcaccgcg ttcaccgtga cccaggccga
    19261 ctgcgatcac gccggcaacc gcgccaccgg ccgcgaccgc gtggtcgtga cctggaagaa
    19321 cgccgaccag acccagagca ccgaccacct cgacgtccgc tactgcgagc ggtgagcgcg
    19381 gcgcgccgat ctaccggcgc ttgatgtcgt gcttggccat catccggatc agcgcgaacc
    19441 gcgacacgcc gagccgcttg gccgcctggg tctggttgcc gtcgctggca tcgagcgcgg
    19501 cgatcaccgc ctcgcgctcg acgctggcga cccgctggcg gacgtcgagc gcgtcgcgcg
    19561 gcggcgtccg ggtctgctcg aggatctgct cgggcaggtg ctcgcggccg atcttgccgc
    19621 cctcgctgag cacgacggcg cgctcgatcg cgttgcgcag ctcgcgcacg ttgccgggcc
    19681 agctcgcgtg ctggagcgcg tcgagcgcgt cgggggtgaa gccgaccgcg ggctggcgca
    19741 ggtcggtcgc gatctcgtgc gcgaagtgga ccgcgagcgg ggcgatctcg ctccggcggt
    19801 cgcgcagcgg cggcaccggg atcacgaacg cggcgatccg gaagaacagg tcctcgcgga
    19861 accggccgcg ctggacctcg atctcgaggt cgcggtgggt cgcgcacacc aggcggacgt
    19921 cgaccggcag ctcggtggtg ccgccgaccc gcacgatccg ccagcgctcg agcacgcgca
    19981 gcagcttgac ctgggtggtc agcggcagct cgccgatctc gtcgaggaac agcgtgccgc
    20041 cggccgccgc ctcgaagaac ccgtgcttgg tggcggtcgc gccggtgaac gcgctcttct
    20101 cgtggccgaa cagctcggac tcgaccaggg tctcggtcag cgccgcgcag ttgagccgga
    20161 cgaacggcgc accggcgcgc gggctcatcc ggtggatcga ctcggcgacg acctccttgc
    20221 cggtgccggt ctcgccgacg atcagcacgc tgatcggcgc ccgcgccgcg cgctcgcccg
    20281 ggtacagcaa cctcgcatcc ccgcgttcct gagtattagg ttcgctcagc gcggggcatg
    20341 cacggacgtg gggacggagc cagcccgtac gggtgcgctc cggacccacg tccaaggtgc
    20401 tcagcgcagc gccctgagac cggccctcac ctcgttcgag tagagctccg gctcctgcca
    20461 ggcggcgaag tggttgcccc ggtcgacctc gttgaagtag atgaggttgg ggtacgcctt
    20521 ctccacccag ctccgcggcg cccggtacat ctccatgggg aagacgctca cggcggcggg
    20581 gacggacacg ttcttgacgt cgaagaagcc gagcttgttc tcccagtaga gacgacccga
    20641 ggagaccccc gtgttcgtca accatgtgag cgtgatgttg tcgaggagct cgtccggcgt
    20701 gaggttgtcc acaggctctc catccacgaa cgcctgcgcg atgtcctcgt agctcctcga
    20761 gtcgtggtcg atcatccaag ccgcaagggc cacgggtgag tccgccaacc cgtagagcgt
    20821 ctggggacgc aacgacaact ccgtcgcgta gccgatgccc ttcgtgtaca cgaagctcag
    20881 gtcctcgtac gcgcgctttt cgtcgtccga gaggccgtcg gggggcgggg cgccggaggg
    20941 gaccgccttc gagatctcgg gtgggacggc gcccggcatg ttggagtgga tgccgagcag
    21001 tccctcgggt gcctgcacac ccatcaggtc cgtgacgatc gcaccccaat cgccgccctg
    21061 cgccgcgaat tcgcggtagc cgagacgctt catcagctcc gcccacgcac gtgcgatgtg
    21121 gtcggggtcc cagccggtgt tcttcggctt ggcggagaac ccgtagccgg gcagcgacgg
    21181 gatcacgaca tggaacgcgt cgtccgcgct gccgccttgg gccgtgtgat cggtgagtgg
    21241 gccgactgcg ttgag
//
LOCUS       DASVOR010000781         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_329045,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000781 DASVOR010000000
VERSION     DASVOR010000781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329045"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1762)
                     /locus_tag="VF516_15450"
     CDS             complement(<1..1762)
                     /locus_tag="VF516_15450"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8109128.1"
                     /translation="MPSLVFLAGPIAGRRYKLADGEYVIGRRSDCQIFVPDMRVSRQH
                     ARLWKTGESWEIEDLGSNNGTFVNGVKVQAATQIRHDDEILIANNRIRVETRESTPEG
                     KIPADGGNAVTIVDVSGGASMIRSREDSGSGRLPIVSSGILSVADQRAVRLLERKLDA
                     LTQILQATAASDTSDALVSKLVDALLDLFPQAEDIGVLVEDERTGELKVQCQRHRKQA
                     FGGDLRVPGTIIQHVLSDRRGVLLREHADENNEGVGARMGAPLIFHGVHYGVVYVESK
                     HAGFRQEDVDLLQTVATQTGLAMHATRVAAQLARREKLERDLRVARQIQRSLLPANVP
                     QVVGLEFAVHYEPAYQIGGDFYDFIWHDPSHLGLTVGDVAGKAISAALYMARLTSELR
                     SRAAIARTPARLLRRVNQEIAQLGDDGIFATLVYCIYDLENRSLVFTNAGHCVPRLRR
                     GDRVFPLQAERAHTPPLGVTSELEAGEARVQLHSGDMLIMVSDGILEARDARGNDYGL
                     SRLSRRIRTARGKVDDVVKAILADIDSHCGEQGQQGDDMTLLAMSIDQRRAKRKTTTL
                     PGVLPGEGGPTRLTTEGGTEA"
     gene            1866..>3126
                     /locus_tag="VF516_15455"
     CDS             1866..>3126
                     /locus_tag="VF516_15455"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8109129.1"
                     /translation="MQALGNFINGAFVAPTGMAVVSRNPAADGAVVFETGMALAAAGD
                     AVRAAAAAQPAWGRLTLAERAGALDRFKQAISARADALADAIVLETGKLRSEARAEIQ
                     TLINRFDLARAAVAAEATGKQVAPGEQLRYQPLGVVGVIGPFNFPLHLCHAHVIPALL
                     TGNTVVVKPSDLTPLCGQRYAEAAQAADLPPGVFNLVIGTGELGAALLEEPAVRGLCF
                     TGSWATGRRILAATLDRPEILVALELGGKNTCVVLDDCAMRQAVHEVVVGGYLSAGQR
                     CTGTERVLVHRKVVDRFVAALAQVARELPFGHPDDPAVFAGPLVSQAALARVDAALDA
                     ARKAGAEAIAVIEASPYGFANAVFTGSPARFEHIYARTRAGIVNRNRSTNLASPKLPF
                     GGVGRSGNYRPTGRGRRATRPRRSPCSR"
BASE COUNT          405 a         1081 c         1172 g          468 t
ORIGIN      
        1 gcgcctcggt gccgccctcg gtggtcagcc gcgtcgggcc gccctcaccg gggagaaccc
       61 ctggtaatgt cgtggttttt cgcttggcgc ggcgctggtc gatcgacatc gcgagcaggg
      121 tcatgtcgtc gccctgctgg ccttgctcgc cgcagtggct gtcgatgtcg gcgaggatcg
      181 ccttgaccac gtcgtcgacc ttgccgcgcg cggtgcggat ccggcgcgac agccgggaca
      241 ggccgtagtc gttgccgcgc gcgtcgcgcg cctcgaggat gccgtccgac accatgatca
      301 gcatgtcgcc ggagtggagc tggacccggg cctcgccggc ctcgagctcc gaggtgacgc
      361 cgagcggcgg ggtgtgggcg cgctcggcct ggagcgggaa cacgcggtcg ccgcggcgga
      421 gccggggcac gcagtggccg gcgttggtga acaccaggct gcggttctcc aggtcgtaga
      481 tgcagtagac cagcgtggcg aagatgccgt cgtcgccgag ctgggcgatc tcctggttga
      541 cgcggcggag cagccgcgcc ggcgtccgtg cgatcgcggc gcgcgaccgg agctcggagg
      601 tcagccgcgc catgtacagc gccgcgctga tcgccttgcc ggcgacgtcg ccgaccgtga
      661 ggccgaggtg cgacgggtcg tgccagatga agtcgtagaa gtcgccgccg atctggtacg
      721 ccggctcgta gtgcacggcg aactccaggc cgacgacctg cgggacgttg gcgggcaaga
      781 ggctgcgctg gatctggcgg gcgacgcgca ggtcgcgctc gagcttctcg cgccgcgcga
      841 gctgggcggc gacccgggtc gcgtgcatcg ccaggccggt ctgggtcgcg acggtctgca
      901 gcaggtcgac gtcctcctgg cggaagccgg cgtgcttgct ctcgacgtag accacgccgt
      961 agtgcacgcc gtggaagatc agcggcgcgc ccatccgggc cccgacgccc tcgttgttct
     1021 cgtcggcgtg ctcgcggagc agcacgccgc ggcgatcgct cagcacgtgc tggatgatcg
     1081 tgccggggac gcggaggtcg ccgccgaacg cctgcttgcg gtggcgctgg cactggacct
     1141 tgagctcgcc ggtgcgctcg tcctcgacca gcacgccgat gtcctcggcc tgcgggaaca
     1201 ggtcgagcag cgcgtcgacc agcttgctga ccagcgcgtc cgaggtgtcg ctggccgcgg
     1261 tcgcctgcag gatctgggtc agcgcgtcga gcttgcgctc gagcaggcgc accgcgcgct
     1321 ggtcggcgac gctgaggatg ccggagctga cgatcggcag ccggcccgac ccggagtcct
     1381 cgcggctgcg gatcatcgat gcgccgccgc tgacgtcgac gatggtgacc gcgttgccgc
     1441 cgtcggccgg gatcttgccc tcgggagtgc tctcgcgggt ctcgacccgg atccggttgt
     1501 tggcgatcag gatctcgtcg tcgtggcgga tctgcgtggc ggcctggacc ttgacgccgt
     1561 tgacgaacgt gccgttgttc gatccgagat cttcgatctc ccacgactcg ccggtcttcc
     1621 acagccgggc gtgctgccgc gacacgcgca tgtcggggac gaagatctgg cagtccgagc
     1681 ggcggccgat cacgtactcg ccgtccgcga gcttgtagcg gcgccctgcg atcgggccgg
     1741 ccaagaacac caacgagggc atccgcgcgg cctaaatagc acagttctca ggaattggcc
     1801 cagacacacc cgggcaggaa ccgcgccgac ttgacccccc gggggtcggg cggtgtactt
     1861 cgccggtgca ggcgctcggt aacttcatca acggcgcgtt cgttgcgccg accggcatgg
     1921 ccgtggtgtc ccggaacccg gcagccgatg gcgcggtggt gttcgagacc ggaatggctc
     1981 tggcggcggc cggggatgcg gtgcgcgccg cggccgcggc gcagccggcg tggggacggt
     2041 tgaccctcgc agaacgggca ggggccctgg accgcttcaa gcaggcaatt tctgcgcggg
     2101 cggacgcgct cgccgatgcc atcgtgctgg agaccggcaa gctgcgcagc gaggccaggg
     2161 ccgagatcca gacgctgatc aaccgcttcg acctcgcgcg cgcggcggtg gccgccgagg
     2221 cgaccggcaa gcaggtcgcg ccgggggagc agctgcgcta ccagccgctc ggcgtggtcg
     2281 gcgtgatcgg cccgttcaat ttcccgctcc acctgtgcca cgcccacgtg atccccgcgc
     2341 tgctcaccgg caacacggtg gtggtgaagc cgagcgatct cacgcccctg tgcggccagc
     2401 gctacgccga ggcggcccag gccgcggact tgccgcccgg ggtgttcaac ctcgtgatcg
     2461 gcaccggcga gctcggcgcc gccctgctgg aggagccggc tgtgcgcgga ctgtgcttca
     2521 ccggcagctg ggcgaccggc cgccggatcc tggccgcgac gctggaccgc cccgagatcc
     2581 tggtcgcgct ggagctcggc ggcaagaaca cctgcgtcgt gctggacgac tgtgcgatgc
     2641 gccaggcggt ccacgaggtc gtcgtgggtg ggtacctgtc ggccggtcag cgctgcacgg
     2701 ggaccgagcg ggtgctggtc caccgcaagg tcgtcgaccg cttcgtcgcg gcgctggccc
     2761 aggtcgcgcg cgagctgccg ttcgggcacc ccgacgaccc ggccgtgttc gccgggccgc
     2821 tggtctcgca ggcggcgctc gccagggtcg acgccgcgct ggacgccgcg cgcaaggcgg
     2881 gcgccgaggc gatcgccgtg atcgaggcca gcccgtacgg cttcgccaac gcggtgttca
     2941 ccggctcgcc ggcgcggttc gagcacatct acgcccggac ccgcgcgggg atcgtcaacc
     3001 gcaaccgctc gaccaacctc gccagcccca agctgccgtt cggcggcgtg ggccggagcg
     3061 gcaactaccg gccgacgggg cgtgggcggc gcgcaacgcg acctcggcgg tcgccgtgct
     3121 cgagaa
//
LOCUS       DASVOR010000782         9377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_183244,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000782 DASVOR010000000
VERSION     DASVOR010000782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9377
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183244"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VF516_15460"
     CDS             <1..892
                     /locus_tag="VF516_15460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109130.1"
                     /translation="AANLKNIRVRTSTIAVQAEATASLTVNNLDIMGIAVTGTGPCST
                     GIALNGAAKLVAMTLTTRDLGATLEARDQSVVAIANATISGNQTCSGSALMSVISTAS
                     FSLSDSVLDGGASGIAIAAQSTSFHATISNTIVRNMQGDGLGGFPNLKASFQMTGGEL
                     SNNKQSGAELGQGTWTFTNVAIRQNKNLAFYLQGANLVMRNCIVVGNGFGIDVYQDSV
                     ADLGTMASPGNNVLQNNSNASVFAESSVPVNAIGNTWNPNIQGADGNGKYITTAAIPG
                     PVAAVNNGNFQISDGCSLSR"
     gene            complement(1393..2067)
                     /locus_tag="VF516_15465"
     CDS             complement(1393..2067)
                     /locus_tag="VF516_15465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109131.1"
                     /translation="MPRMRTLLFLSLVLVGCSDNSNNNPPADAATPDTTTVALDCPSY
                     CTEIQANCTGANAQYPTETTNAHCLAACASFPTGTLADTSMNTLGCRIYHAGAPAKSD
                     PATHCPHAGPGGDLTSVTSPPGTCGDACTSFCTLEIKACGTTTSAPTTGQYADMNACI
                     TACKAFPNSTHKYTITSAGDSLACRLYHATNAAITGNAATHCPHTGPTPAGATNPCLA
                     GSAATP"
     gene            2397..2765
                     /locus_tag="VF516_15470"
     CDS             2397..2765
                     /locus_tag="VF516_15470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HEX8109132.1"
                     /translation="MMRPLLPENRVDPAIREAIASNHRDIIDEVERAVAAHDVVVVGM
                     KQNPFPRRARKALTAAGIPFHYLEYGSYLNTWRRRTALKIWSGWPTFPMIFVKGVLIG
                     GAEDVQKWIDSGELAKRLGR"
     gene            complement(2879..4825)
                     /gene="htpG"
                     /locus_tag="VF516_15475"
     CDS             complement(2879..4825)
                     /gene="htpG"
                     /locus_tag="VF516_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HEX8109133.1"
                     /translation="MSQAPAPAGSPGSTERHEFQAEVKQLLDLMVHSLYSDKDIFLRE
                     LISNASDALDKLRFEQLTRTELGASDLHIRIDVDRAARTVSVSDNGIGMTRDEVVQNI
                     GTIARSGTREFLSSLDEAQKKNVPPELIGQFGVGFYSAFMVADRITLVTRRAGQTAAT
                     RWESTGDGSYTLADAERPTQGTTVTLHLKPSGPDPEHGRDYTADQVLKDIVKRYSDFV
                     TYPIRMQRWRDGDKPSDPKILEDETLNSMKAIWDRPRGEVTDTEYKEFYRHVAHDWTD
                     PLRSIPIKIEGTLEAYALLYIPSKAPFDLYSAEMKRGVQLYVKRVFVMDECKELMPTY
                     LRFVKGVVDAHDLSLNVSREILQKDRQIQVIRKQLVKKVLSTLDEMKRDKPDDYLPFW
                     AAFGPVLKEGLLAFDTADQDRLLDLVITHSTHAADKLASLDDYVGRMKEGQDAIYFLT
                     GPSYEAIARSPLLEAFTAKGYEVLLFTDPVDELWLERAPRFKDKPLTSIGRGEVKLGS
                     DDERKQAAETLDEKQKEFADLLAFFRVQLQNEVKEVRLSSRLTSSPVCLVSDEHDLTP
                     RMQKMLEQLGQKPPKIKPILELNPGHPLLPKLHALFASNKADPRLATYAEVLLGQAHL
                     ADSGQLPDPAGFARALGDLMLHGV"
     gene            4807..5877
                     /locus_tag="VF516_15480"
     CDS             4807..5877
                     /locus_tag="VF516_15480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8109134.1"
                     /translation="MQGLGSWLVGRLVSRTPARRNGSVRKNSRLGNAPPHHRGLGGRM
                     EAIAIDKLSKSYGRINALSNVSFKIEPGEVIGLLGPNGAGKTTLMKILTGYLEPDTGD
                     VQVHGIDVVSDPLAAQRRIGYLPESAPLYGEMLVQEYLEMMAAMRGVPEERRRDRMVE
                     VIRATGLTDRVVQPISTLSKGFRQRVGIAQAIIHEPDILILDEPTTGLDPAQIVEIRD
                     LIVGLAKKSTVLLSTHILSEVEATCERVLVIMQGALRADAKLADLRNANAAVVAIEAA
                     ASGVADVLNKVAGVTSVEAAGTAGDFQRWRVTSGSKDDLCPALFDALRTTRWKIGELR
                     PEPKTLERVFRDLAERAGQVAP"
     gene            5874..8684
                     /locus_tag="VF516_15485"
     CDS             5874..8684
                     /locus_tag="VF516_15485"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEX8109135.1"
                     /translation="MIGTVVKKELRSYFNSAIAVIFLAAFLLVALFTFFVAEKFFARG
                     LADLRPLFKWMPRLLIILVSALSMRQWADERRVGTLEVLLTLPVPRWQLVVGKFLAGM
                     VLIAIALGLTLILPITIAHMGNLDLGPVVGGYLATLLLAAAYLVIGMCVSAATDNEIV
                     AFIGTALVCGVADVVGYVAGDIGRLFGTEARFESVARGVLDLRDLAYYGGIVAVGIAL
                     NVLLLDRLTWSRGPRGKTRRSAAMLAVALVAANAVGLDVWMAPIRRVRLDLTQDGAYS
                     LSDSTRKILGGLDERLLIRGYFSEKSHPKLEPLVPQLRDLLEEYRIAGGNKVRVEFVD
                     PTDSEDAKREAKERFGIDPTPLRFATQTEKTVVNAYFAIAIEYGDQHAVIGLDELIAV
                     RVMDIGELEISLKNPEYQLTKTIKKTVAEFSSVDQLFASTAGKIQLTAYVTPKTLPEN
                     WKDGPAKLQKVVDELTKQAGGKLAYATVEPKTEAEMRDLFSKFGLRPHQELFSSQVFY
                     FDVLLQVGNRLVRINPPDKLGDAELKAAVIEGLKRAAPGFTRVVGLWSPPQGPPTPMM
                     QGMPPQQMPPPQSFRSLQRALAGNYEVRDVQLTAPVPDDVEALVVAGPASLDAKAAEH
                     LDQFVMRGGALVLLDGRFRLAPGAGLSVEKVTTGLESMLQKWGVAVEDQMVMDSKSDV
                     FPVPENRDLGNGMVVREIQQLPYPYFVKMDGGQVASSSMITGGLTGSVMHWASPVKAD
                     AKAGDDEHRVEVLLRSSDGSWLTTSTQVEPNARTYPKTGFPGPKDTDKKGSQVMAVAI
                     TGGFASSVEKPKDKPKDPADKDAAPLIQHSPPDTRIVVFGSSAFVSDDVLGLAQQLES
                     DLATSNLELVHNAVDWSLADTDLLGIRSRNAAARALTVSTQSRGTWAVVNAAIALLGL
                     IAVVGFAWLRRRSVQPIISKEA"
     gene            8686..>9377
                     /locus_tag="VF516_15490"
     CDS             8686..>9377
                     /locus_tag="VF516_15490"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEX8109136.1"
                     /translation="MLTRFHKILIAALGVQLVLAVIVLTRGDDSAALKERPILAGFDA
                     AKVTRLQVFGKLAGATDAAKPDAKPLDLVKRDKDWVLASGFDYPVEQKKLDDVLSPIA
                     KLAAAAPIATQAARHKQLHVADDDFDRKLVITAGGKDVTLFVGAPAGARRSAVRLGGD
                     DKVYAVAGLTAFAIGIEPRQWIDTAYVKIPRDDVHKLVVQRDGKTVEMTKAAPPSPPA
                     AGSGSAAGSGSGA"
BASE COUNT         1565 a         2817 c         3443 g         1552 t
ORIGIN      
        1 agccgcgaat ctcaagaata ttcgagttcg caccagcacg atcgcagtac aagccgaagc
       61 gactgccagc ctgacagtca ataacctgga catcatgggt attgctgtca caggtactgg
      121 tccatgctcc acagggatcg ctctcaatgg agctgcaaag ctggtcgcga tgaccttgac
      181 cacgcgcgat cttggcgcaa cgctggaggc cagagaccag agcgtcgtgg ccatcgcaaa
      241 tgcgaccata tctggaaatc agacctgttc aggttccgcg ctcatgtcgg ttatatcgac
      301 agcgagtttt agtctgagcg acagcgttct ggatggtgga gccagtggga ttgcaatcgc
      361 tgcccaatcg acatcatttc acgctacaat atcgaatacg atcgttcgaa acatgcaagg
      421 tgatgggttg ggcggattcc caaatctcaa ggcgtcgttt caaatgactg gcggtgagtt
      481 gtcaaataat aaacaaagtg gtgctgagtt gggacaaggc acctggacat ttacaaatgt
      541 agctatccga cagaacaaga acctagcatt ctacttgcaa ggtgctaact tggtcatgcg
      601 gaattgtatt gttgtcggaa atggatttgg tatagatgtg tatcaggatt cagtagccga
      661 tctcggaaca atggcgagtc caggaaataa tgtgctccag aacaattcga acgcaagcgt
      721 gttcgcggag tccagtgttc cagtcaatgc gatcggtaac acatggaatc ctaatataca
      781 gggcgcggac ggaaatggaa agtacataac aactgctgct atcccaggac cagtggcagc
      841 ggtcaataat ggcaattttc agataagtga tggctgtagt ctctcgcggt aataagcgcg
      901 atcccgcttc cccgcggata gtccacgtcg cgcgaggcgc tgcagtatca ccaggggctg
      961 cttgacacta ctgcgtcagg ttggggcggc cggcgctcac cgcagcgcgg cgcatggcac
     1021 gctggcgatg gcttgcgcga tcgcgagccc gccgagccag cggggccgcg aggtgagcag
     1081 gagcgcgctc atccgtcacg ctctgccgac ggcaccgtcc cgaccccccc gctgcttcct
     1141 cgccgaccgc agtggctctc gtcaagtgat cgatcttact cccgtcgagc actgacatga
     1201 aagcgccgca cacgtacgca gtaccccttg agggcggtag accaagaaca ttacgctttg
     1261 cggtccatcc tcgcggcctg atgcggagcc gacggtcgcg ggtccatgtc ggacacgaaa
     1321 gagctctaga aaaagcgcgt cgcgagctgg cgagtacgtg gtgagactgc cgagaagtac
     1381 gaatccagtg gctcagggcg tggctgccga gccagcaagg catgggttgg tggcgcccgc
     1441 cggcgtgggt cccgtgtgag ggcagtgggt cgcggcgttg cccgtgatgg ccgcgttggt
     1501 ggcgtggtac aggcggcatg ccagcgaatc gcctgcagag gtgatcgtgt acttgtgggt
     1561 cgagttgggg aacgccttgc acgcggtgat gcacgcgttc atatcggcgt actgaccggt
     1621 cgtgggcgcc gacgtcgtcg taccgcaggc cttgatctcg agcgtgcaga agctggtgca
     1681 ggcatcgccg cacgtgcccg gaggcgaggt gacactcgtg aggtcaccac cggggccggc
     1741 gtgaggacag tgggtggcgg gatcactctt cgcaggagcg ccggcgtgat aaatccggca
     1801 gccgagcgtg ttcatggagg tgtcggcgag cgtgccggtg gggaacgaag cgcaagccgc
     1861 caagcaatgg gcgttggtcg tttcggtcgg gtactgagcg ttcgcaccag tgcagttggc
     1921 ctggatctcg gtgcagtagc tggggcagtc cagcgcgacg gttgtcgtgt cgggcgtggc
     1981 cgcgtcggcc ggcgggttgt tgttgctgtt gtcgctgcag ccgacgagga caagcgagag
     2041 gaataggagg gttcgcatgc gcggcatcgt cggagaggtc gctgccgatc gcaactatct
     2101 cccgccgcga atattgcgaa acgttgctaa ctcgggtcac acccggtcag ggcggcgtgt
     2161 agggggtggc gtcgccgagg acggcgatgc agatcgctgt gcgtctggac acaggctcgg
     2221 aacacggccg tgaccgcgtg cgcagtcgtc gaggcgtgtt gccccgtcga gagcctgcgc
     2281 tggagagatc tgtcagggtt gaatatttcg gtaatccgcg aaaccatcct ggctgctcgg
     2341 cttcgacggt ggcccgtcgg gaagcgggcg ttcgcgggtc gctgtagtgt cggcgcatga
     2401 tgagaccgct gctgcccgaa aaccgcgtgg atcctgcgat tcgcgaggcg atcgcctcga
     2461 accaccgcga catcatcgac gaggtcgagc gcgcggtggc ggcgcacgac gtggtcgtgg
     2521 tcgggatgaa gcagaacccg tttccgcgcc gggcgcgcaa ggcgctgacc gcggcgggaa
     2581 tcccgttcca ctacctcgag tacggcagct acctgaacac ctggcggcgg cggacggcgc
     2641 tcaagatctg gagcggctgg ccgacgttcc cgatgatctt cgtgaagggg gtgctgatcg
     2701 gcggcgccga ggatgtgcag aagtggatcg actcggggga gctggcgaag cggctcgggc
     2761 gctgacgggg gccggctggc tggtcgggcc gcgagcaggc gcgtaactgc tgcggcggtg
     2821 ccggcagagg agtcgggcag cgcggccgac gcaaccaacg ccccccggcc ggcgcgggtc
     2881 acacgccgtg gagcatgagg tcgccgaggg cgcgggcgaa gccggccggg tcggggagct
     2941 ggccggagtc ggcgaggtgg gcctggccga gcaggacctc ggcgtaggtg gcgaggcgcg
     3001 ggtcggcctt gttggaggcg aacagggcgt ggagcttggg gagcagcggg tggccggggt
     3061 tgagctcgag gatcggcttg atcttgggcg gcttctggcc cagctgctcg agcatcttct
     3121 gcatccgcgg ggtgaggtcg tgctcgtcgc tgaccaggca gaccggcgac gaggtgaggc
     3181 ggctggacag gcggacctcc ttgacctcgt tctggagctg gacccggaag aacgcgagca
     3241 ggtcggcgaa ctccttctgc ttctcgtcga gcgtctcggc ggcctgcttg cgctcgtcgt
     3301 cggagccgag cttgacctcg ccgcggccga tcgaggtgag gggcttgtcc ttgaacctgg
     3361 gggcgcgctc gagccagagc tcgtcgaccg ggtcggtgaa caggaggacc tcgtagccct
     3421 tggcggtgaa cgcctcgagg aggggggacc tggcgatcgc ctcgtaggac gggccggtga
     3481 ggaagtagat cgcgtcctgg ccttccttca tgcggccgac gtagtcgtcg agcgaggcca
     3541 gcttgtcggc ggcgtgggtc gagtgggtga tgacgaggtc gagcagcctg tcctggtcgg
     3601 cggtgtcgaa cgcgagcagg ccctccttga gcacggggcc gaacgcggcc cagaacggga
     3661 ggtagtcgtc gggcttgtcg cgcttcatct cgtcgagggt ggacaggacc ttcttgacca
     3721 gctgcttgcg gatgacctgg atctggcggt ccttctggag gatctcgcgc gagacgttga
     3781 gcgagaggtc gtgggcgtcg accacgccct tgacgaagcg caggtaggtc ggcatcagct
     3841 ccttgcactc gtccatgacg aagacgcgct tgacgtagag ctggacgccg cgcttcatct
     3901 cggcgctgta caggtcgaac ggggccttgc tcgggatgta gagcagggcg taggcctcga
     3961 gggtgccctc gatcttgatc gggatcgagc gcagcgggtc ggtccagtcg tgggcgacgt
     4021 ggcggtagaa ctccttgtac tcggtgtcgg tgacctcgcc cctggggcga tcccagatcg
     4081 ccttcatcga gttgagggtc tcgtcctcga ggatcttggg gtcggacggc ttgtcgccgt
     4141 cgcgccagcg ctgcatccgg atcggatagg tgacgaagtc ggagtagcgc ttgacgatgt
     4201 ccttgagcac ctggtcggcg gtgtagtcgc gtccgtgctc cggatccgga ccagagggct
     4261 tgaggtggag ggtgacggtg gtgccctggg tggggcgctc ggcgtcggcg agggtgtagg
     4321 agccgtcgcc ggtcgactcc cagcgggtcg cggcggtctg accggcgcgg cgggtgacga
     4381 gggtgatgcg atcggcgacc atgaaggcgg agtagaagcc gacgccgaac tggccgatca
     4441 gctcgggcgg cacgttcttc ttctgagcct cgtccaggga gctcaggaac tcgcgggtgc
     4501 cggaccgcgc gatcgtgccg atgttctgga cgacctcgtc gcgggtcatc ccgatgccgt
     4561 tgtcggacac cgagacggtg cgcgcggcgc gatcgacgtc gatccggatg tggaggtcgg
     4621 aggcgccgag ctcggtccgg gtcagctgct cgaaccgcag cttgtcgagc gcgtcggacg
     4681 cgttggagat cagctcgcgg aggaagatgt ccttgtccga gtacagcgag tggaccatga
     4741 ggtcgaggag ctgcttgacc tcggcctgga attcatggcg ttcggtagat cccggggatc
     4801 cggcgggtgc aggggcttgg ctcatggctc gtgggcaggt tagtaagcag gacgccggcc
     4861 aggcgaaacg ggtccgttcg aaaaaattcc cggctcggga atgcaccacc gcatcaccgc
     4921 ggtcttgggg ggcgcatgga agcgatcgcg atagacaagt tatccaagag ttacgggcgg
     4981 ataaacgctc tgtcgaacgt cagcttcaag atcgagccgg gcgaggtcat cggcctgctc
     5041 ggccccaacg gggccggcaa gacgacgctc atgaagatcc tgaccggcta cctggagccg
     5101 gataccgggg acgtccaggt gcatggcatc gacgtcgtgt cggatccgct cgccgcgcag
     5161 cgccggatcg gctacctgcc ggagagcgcg ccgctgtacg gcgagatgct ggtccaggag
     5221 tacctggaga tgatggcggc gatgcgcggg gtgcccgagg agcgccgccg cgatcgcatg
     5281 gtcgaggtga tccgggcgac cggtctgacc gatcgggtgg tccagccgat ctcgaccctg
     5341 tccaagggct tccggcagcg ggtcgggatc gcccaggcga tcatccatga accggacatc
     5401 ctgatcctgg acgagccgac gaccggcctc gatccggcac agatcgtgga gatccgcgac
     5461 ctgatcgtcg ggctggccaa gaagtcgacc gtgctgctgt cgacgcacat cctgtcggag
     5521 gtcgaggcga cctgcgagcg ggtgctggtg atcatgcagg gcgcgctgcg cgccgacgcc
     5581 aagctcgccg acctgcgcaa cgccaacgcc gcggtggtcg cgatcgaggc cgcggcgagc
     5641 ggggtcgcgg acgtgctgaa caaggtggcc ggggtgacgt cggtcgaggc cgcgggcacc
     5701 gccggcgact tccagcgctg gcgggtgacg tccgggagca aggacgacct gtgcccggcg
     5761 ctgttcgacg cgctgcgcac gacgcgctgg aagatcggtg agctcagacc cgagcccaag
     5821 acgctggagc gcgtgttccg cgacctcgcg gagcgcgcag gacaggtggc gccatgatcg
     5881 gtaccgtcgt caagaaagag ctgcggagct acttcaactc cgcgatcgcg gtgatcttcc
     5941 tggccgcgtt cctgctggtc gcgctgttca cgttcttcgt cgcggagaag ttcttcgcgc
     6001 gcgggctggc ggatctgcgg ccgctgttca agtggatgcc gcggctcttg atcatcctgg
     6061 tgtcggcgct gtcgatgcgg cagtgggccg acgagcgccg ggtcggcacg ctcgaggtgc
     6121 tgctgacctt gccggtgccg cgctggcagc tggtggtcgg caagttcctc gccggcatgg
     6181 tgctgatcgc gatcgcgctc gggctgacgc tgatcctgcc gatcacgatc gcgcacatgg
     6241 gcaacctcga tctcgggccg gtggtcggcg gctacctggc gacgctgctg ctggcggcgg
     6301 cgtacctggt gatcgggatg tgcgtgtcgg cggcgaccga caacgagatc gtcgcgttca
     6361 tcggcacggc gctggtgtgc ggggtcgcgg acgtggtcgg gtacgtcgcc ggtgacatcg
     6421 gccggctgtt cggcaccgag gcgcggttcg agagcgtggc gcgcggcgtg ctcgacctgc
     6481 gcgacctcgc ctactacggc gggatcgtcg cggtcggcat cgcgctgaac gtgctgctgc
     6541 tcgaccggct gacctggagc cgcggtccgc gcggcaagac ccggcgcagc gccgcgatgc
     6601 tcgcggtggc gctggtcgcc gcgaacgcgg tcgggctcga cgtctggatg gcgccgatcc
     6661 gccgggtccg gctcgacctg acccaggacg gcgcgtacag cctgtcggac tcgacccgca
     6721 agatcctggg cgggctcgat gagcggctgc tgatccgggg ctacttctcg gagaagagcc
     6781 accccaagct cgagccgctg gtgccgcagc tgcgcgatct gctcgaggag taccggatcg
     6841 cgggcggcaa caaggtccgg gtcgagttcg tcgatccgac cgactccgag gacgccaagc
     6901 gcgaggccaa ggagcggttc gggatcgacc cgacgccgct ccggttcgcg acccagaccg
     6961 agaagacggt ggtcaacgcg tacttcgcga tcgcgatcga gtacggcgac cagcacgcgg
     7021 tgatcgggct cgacgaactg atcgcggtgc gcgtgatgga catcggcgag ctcgagatct
     7081 cgctgaagaa ccccgagtac cagctgacca agacgatcaa gaagacggtc gccgagttct
     7141 cgagcgtcga ccagctgttc gcgtcgaccg cgggcaagat ccagctgacc gcgtacgtca
     7201 cgccgaagac gctgcccgag aactggaagg acgggccggc caagctgcag aaggtggtcg
     7261 acgagctgac caagcaggcc ggcggcaagc tggcctacgc gacggtcgag cccaagaccg
     7321 aggccgagat gcgtgacctg ttcagcaagt tcgggctgcg cccgcaccag gagctgttct
     7381 cgagccaggt gttctacttc gacgtgctgc tccaggtcgg caaccggctg gtccggatca
     7441 acccgccgga caagctgggc gacgccgagc tcaaggccgc ggtgatcgag gggctcaagc
     7501 gcgccgcgcc ggggttcacc cgggtggtcg ggctgtggtc accgccgcag ggaccgccga
     7561 cgccgatgat gcagggcatg ccgccgcagc agatgccgcc gccgcagtcg ttccgcagcc
     7621 tgcagcgcgc gctggcgggt aactacgagg tccgcgacgt ccagctgacc gcgccggtgc
     7681 ccgacgacgt cgaggcgctg gtggtcgcgg ggccggcgtc gctcgacgcc aaggcggccg
     7741 agcacctcga ccagttcgtg atgcgcggcg gcgcgctggt gctgctcgac ggccggttcc
     7801 ggctcgcccc gggcgccggg ctgagcgtcg agaaggtgac gaccgggctc gagtcgatgc
     7861 tccagaagtg gggcgtcgcg gtcgaggacc agatggtgat ggacagcaag agcgacgtgt
     7921 tcccggtgcc ggagaaccgc gatctcggca acggcatggt cgtgcgcgag atccagcagc
     7981 tgccgtaccc gtacttcgtc aagatggacg gcggccaggt ggcgtcgtcg agcatgatca
     8041 ccggcgggct gaccggctcg gtgatgcact gggcatcgcc ggtcaaggcc gacgccaagg
     8101 ccggcgacga cgagcaccgg gtcgaggtgc tgctgaggtc gtcggacggg tcgtggctga
     8161 ccacgtcgac ccaggtcgag cccaacgcgc ggacctaccc caagaccggc ttccccggtc
     8221 ccaaggacac cgacaagaag ggcagccagg tgatggcggt ggcgatcacc ggcgggttcg
     8281 cgagcagcgt ggagaagccc aaggacaagc ccaaggaccc ggccgacaag gacgccgcgc
     8341 cgctgatcca gcactcgccg ccggataccc ggatcgtcgt gttcggatcg tcggcgttcg
     8401 tgtcggacga cgtgctcggg ctcgcccagc agctcgagtc ggacctcgcg acgtccaacc
     8461 tcgagctggt gcacaacgcg gtcgactggt cgctggccga caccgacctg ctcgggatcc
     8521 ggtcgcgcaa cgcggcggcg cgggcgctga ccgtgagcac ccagtcgcgc ggcacgtggg
     8581 ccgtcgtgaa cgccgcgatc gcgctgctcg ggctcatcgc ggtggttggc ttcgcctggc
     8641 tgcggcggcg ctccgtccag cccatcatct cgaaggaggc gtgagatgct cacgcggttt
     8701 cacaagatcc tgatcgcggc gctgggggtc cagctggtgc tcgccgtgat cgtgttgacc
     8761 cgcggcgacg acagcgccgc gctgaaggag cgcccgatcc tcgccgggtt cgacgcggcg
     8821 aaggtgacgc ggctgcaggt gttcggcaag ctggccggcg cgaccgatgc cgccaagccg
     8881 gacgccaagc cgctcgacct ggtcaagcgc gacaaggact gggtgctggc gtcggggttc
     8941 gactacccgg tcgagcagaa gaagctcgac gacgtgctgt cgccgatcgc caagctggcg
     9001 gccgcggcgc cgatcgcgac ccaggccgcc cggcacaagc agctgcacgt cgccgacgat
     9061 gacttcgacc gcaagctggt gatcacggcc ggcggcaagg acgtcacgct gttcgtcggc
     9121 gccccggcgg gcgcgcggcg cagcgcggtg cggctcggcg gcgacgacaa ggtctacgcg
     9181 gtggccgggc tgaccgcgtt cgcgatcggc atcgagccgc ggcagtggat cgacaccgcc
     9241 tacgtcaaga tcccgcgcga cgacgtccac aagctcgtgg tccagcgcga cggcaagacc
     9301 gtggagatga cgaaggccgc accgccttcg ccgccggccg cggggagcgg gagcgcggcg
     9361 ggcagcggga gcggggc
//
LOCUS       DASVOR010000783         3483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_475385,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000783 DASVOR010000000
VERSION     DASVOR010000783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475385"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1626)
                     /locus_tag="VF516_15495"
     CDS             complement(<1..1626)
                     /locus_tag="VF516_15495"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HEX8109137.1"
                     /translation="MCPDALPWIAVAIAATLAACGPTPPAGTGCRAALLPGDLVITEV
                     FADYQASGQASGGATGTDTGKEWFEIYNATDAAIDLSGLTLTASRGDGSRPRTHTMRE
                     ARIAPGQYFTLGSAAPGQTPAYVDYGYGTDLGDLANTDGKLALACGDAEIDSATYTAV
                     RPGHARELTGAQPPDYTLNDDPDSWCQATAAEFEAGNFGTPGAANDCRPVIAGQCSDG
                     GAQRSAVPPGPGDLVITEVMASPTRVSDAAGEWLEARVMTDVDLVGVGVGRIAGTPDV
                     IAGPDCHPVRAGSYVVFARSADPAVNGGLPAAAIAGTFRFNLISGSTTAPGDVEIIAG
                     TTVVDAVTWTHSTSGAALQLDPGRIDPSANDAESNFRDATQPYGAGDLGTPGAGNAQC
                     ALLPGPGMCDDGGRIRAIVPPRPGELVISEILANPANVAGQTDATREWFEVASTGAAA
                     FDLNELGVGRIGAAGAQVLSARCIPVAPHGFAVFARSADPALDGMLPVVHATFKLGLV
                     DSNGDLQIAQGATVLDAVRWTSVTPGVARQLDPG"
     gene            complement(1687..2067)
                     /locus_tag="VF516_15500"
     CDS             complement(1687..2067)
                     /locus_tag="VF516_15500"
                     /inference="COORDINATES: protein motif:HMM:NF014168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8109138.1"
                     /translation="MRAIVPVGRVLFALIFVASVVGHFSSAEISEAAAHGVPLATILV
                     PLSGLIALVGGVSVMLGYRARFGALLLLVFLVPVTLIMHKFWGLPDPQMAMMQKIQFM
                     KNTSLIGACLLLMYHGSGPYSLDG"
     gene            complement(2137..3069)
                     /locus_tag="VF516_15505"
     CDS             complement(2137..3069)
                     /locus_tag="VF516_15505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109139.1"
                     /translation="MRRPMTSCILLLASCGAHISSNPEDEHGSLDAGVGVDASTPGHD
                     AAPAADAAPLGPWSAPAAVTVAATGAIEDDVTLTSNALEMVYAIADPTTGKKDLYYTS
                     RTAIGAPWAPVTKLPFDRDTSSEETPRFSPDDKTLYFASDRAADDGNLDIYAVTHAAT
                     TDALWGLPRPVAPFATPATEKWLSPCSDGHYVVVQSTAAGDTDLFEGTLGSAAPPAPI
                     DVLNTTMTETGAFLTADCLTVYFASFRVTPEKIFVSHRASVGSPWQPPAPVDDFKIAG
                     GNGAQEDPWLSPDGRTFAFASDAAGTKDVYLSTR"
     gene            3272..>3483
                     /locus_tag="VF516_15510"
     CDS             3272..>3483
                     /locus_tag="VF516_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109140.1"
                     /translation="MKNTLGSLLLTTLLLGGGAGCVVRARAAVVAEPPPPPVAVVEVE
                     SEPPPPRVVVMEPRPGFLFIEGRWNWS"
BASE COUNT          473 a         1190 c         1314 g          506 t
ORIGIN      
        1 gccgggatcg agctggcggg cgacgcccgg ggtcaccgac gtccagcgca cggcgtcgag
       61 cacggtcgcg ccctgggcga tctggaggtc gccgttgctg tcgaccaggc cgagcttgaa
      121 cgtcgcgtgg accaccggca gcatgccgtc gagcgcgggg tcggccgagc gggcgaacac
      181 cgcgaacccg tgcggcgcca ccgggatgca gcgcgccgac aggacctgcg cgccggccgc
      241 gccgatccgg ccgacgccga gctcgttgag gtcgaacgcg gcggcgccgg tgctggcgac
      301 ctcgaaccac tcgcgggtgg cgtcggtctg gccggcgacg ttcgccgggt tggcgaggat
      361 ctcgctgatc acgagctcgc cggggcgcgg cgggacgatc gcgcggatcc ggccgccgtc
      421 gtcgcacatc ccggggccgg gcaacagggc gcactgcgcg ttgccggcgc cgggcgtgcc
      481 gaggtcgccc gcgccgtacg gctgggtcgc gtcgcggaag ttgctctcgg cgtcgttggc
      541 gctcggatcg atgcgacccg ggtcgagctg gagcgcggcg ccgctggtcg agtgggtcca
      601 ggtcaccgcg tcgaccacgg tggtcccggc gatgatctcg acatcgcccg gcgcggtggt
      661 cgatccgctg atcaggttga accggaaggt cccggcgatc gccgccgcgg gcagcccgcc
      721 gttgaccgcg gggtcggccg agcgcgcgaa caccacgtag ctgccggcgc ggacagggtg
      781 gcagtcgggg ccggcgatga cgtccggcgt gccggcgatc cggccgacgc cgaccccgac
      841 gaggtcgacg tcggtcatca cccgggcctc gagccactcg ccggcggcgt cgctgacccg
      901 ggtcgggctc gccatcacct cggtgatcac caggtcgccc ggccccgggg gcaccgcgct
      961 gcgctgcgcg ccgccatcgc tgcactggcc ggcgatcacc ggccggcaat cgttcgccgc
     1021 gcccggcgtc ccgaagttgc cggcctcgaa ctcggcggcg gtggcctggc accagctgtc
     1081 cggatcgtcg ttgagcgtgt aatccggcgg ctgcgcgccg gtgagctcgc gcgcgtggcc
     1141 gggccggacc gccgtgtagg tcgcgctgtc gatctccgcg tcgccgcacg ccagcgccag
     1201 cttgccgtcg gtgttcgcca ggtcgccgag gtcggtgccg tagccgtagt cgacgtaggc
     1261 gggcgtctgg ccgggcgccg cgctgcccag cgtgaagtac tggccgggtg cgatgcgggc
     1321 ctcgcgcatc gtgtgcgtgc gcggccgcga gccgtcgccg cggctcgccg tgagggtgag
     1381 tccggacaga tcgatcgccg cgtccgtggc gttgtagatc tcgaaccact cttttccggt
     1441 gtcggtgccg gtggcgccgc cggaggcctg gccggaagcc tggtagtccg cgaatacctc
     1501 ggtgatcacc aggtcgcccg gcagcagggc ggcccggcag cctgtgccgg cgggcggcgt
     1561 cggaccgcac gcggcgagcg tggcggcgat cgcgacggcg atccagggca gggcgtcggg
     1621 gcacacgccg ggccatctat cgtattcgcg ctcgggtgtc gagcgagtcg tcctcgcggg
     1681 cctggtctag ccgtcgaggc tgtacggccc cgagccgtgg tacatgagga gcaagcacgc
     1741 gccgatcagc gacgtgttct tcatgaactg gatcttctgc atcatcgcca tctgcggatc
     1801 cggcaggccc cagaacttgt gcatgatcag ggtcacgggc acgaggaaca ccaggagcag
     1861 cagggcgccg aaccgggcgc gatagcccag catcacgctg accccaccga ccagcgcgat
     1921 cagcccggag agcggaacga ggatggtggc gagcggcacg ccgtgcgctg ccgcctcgga
     1981 gatctccgca gacgagaagt gcccgacgac gctggcgacg aagatcaacg cgaacagcac
     2041 ccggccgaca gggacaatgg ctcgcatgcc gggcgaagtt gcaaccgaca tgcccgcacc
     2101 ggccggcggc tggcatggcc gtggcacacg ccccggtcag cgcgtgctga ggtagacgtc
     2161 cttggtgccg gcggcgtcgc tggcgaacgc gaacgtgcgg ccgtcgggcg acagccacgg
     2221 atcctcctgc gcgccgttgc cgccggcgat cttgaagtcg tcgacgggag ccgggggctg
     2281 ccacggcgag ccgacgctcg cgcggtgcga gacgaagatc ttctccggcg tgacgcggaa
     2341 cgaggcgaag tacacggtca ggcaatcggc ggtgaggaac gcgccggtct cggtcatggt
     2401 cgtgttgagc acgtcgatcg gcgccggcgg cgcggcgctc ccgagcgtgc cctcgaacag
     2461 gtcggtgtcg ccggccgccg tgctctggac gaccacgtag tgaccgtcgc tgcacggcga
     2521 cagccacttc tcggtcgccg gggtggcgaa tggcgcgacc ggccggggca gtccccacag
     2581 cgcatccgtg gtcgccgcgt gggtcaccgc gtagatgtcg aggttgccgt cgtccgccgc
     2641 gcggtcggac gcgaagtaca gcgtcttgtc atcgggagaa aatctcggag tctcctcgct
     2701 gctggtgtcg cggtcgaacg gcagcttggt gaccggcgcc cacggcgcgc cgatcgcggt
     2761 gcgcgacgtg tagtacagat ccttctttcc ggtcgtcggg tcggcgatcg cgtacaccat
     2821 ctccagcgcg ttcgaggtca gggtgacgtc gtcttcgatc gctccggtcg cggcgacggt
     2881 caccgcggcc ggggccgacc acgggccgag cggcgcggcg tcggcggccg gtgccgcgtc
     2941 atggccggga gtgctcgcgt ccacgccgac accggcatcg agcgagccgt gctcgtcctc
     3001 cgggttgctg ctgatgtgtg caccgcatga cgcgagcagc aagatgcacg acgtcatggg
     3061 ccggcgcatg cggcgatggt aacgtagggc ccccgcagcc cggagcagcg atgcagcacc
     3121 gatgtcgtac aagtcacgca gggcagactg ccccgatgtg gtgtgaagac gacaggcccg
     3181 tggctacacc cgtcggcaga ggcccgcgga atgtctggca atctgcctgg tctctggatt
     3241 gctctgcaga tcgagcgcaa ggaggatcac aatgaagaac acactcggta gcttgttgct
     3301 caccaccctg ttgctcggcg gcggtgccgg ctgcgtggtt cgcgcgcggg cggccgtcgt
     3361 cgcggagccg ccgccgccgc cggtcgccgt cgtcgaggtc gagtcggaac cgccaccgcc
     3421 gcgcgtggtc gtcatggagc cgcggcccgg cttcctcttc atcgagggcc gctggaactg
     3481 gtc
//
LOCUS       DASVOR010000784         3370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_329086,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000784 DASVOR010000000
VERSION     DASVOR010000784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329086"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF516_15515"
     CDS             complement(<1..442)
                     /locus_tag="VF516_15515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109141.1"
                     /translation="MPADAASTGPPRGPDRRGLRADCARCFGLCCVAPAFAASSDFAI
                     DKPAGQACPHLQASFRCAIHPRLRQLGFPGCTVYDCFGAGQHVSQVTFGGKDWRSAPD
                     TAQRMFDVFAIMRQLHELIWYLCEARELAAARPLHAALGDACDAT"
     gene            654..1895
                     /locus_tag="VF516_15520"
     CDS             654..1895
                     /locus_tag="VF516_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8109142.1"
                     /translation="MHRLWWVALATIAIASSAAAADPTEIGGWFGPRLFSSSSGLGYL
                     DDAPAHPALQNGIAFGGRVARPFLPWLVPELELALMPTSTSAVGGARAASVLWFEPRL
                     HLRFELWQGRRLQPFVLAGGGSPVSLSTASKTYGTSITGDGYLGGGVRFATQKGFDFR
                     FDARIALLPSQPGSGFGLTPELDFGIGIEFHVGEPRPSAPDEARLAQAPPPDRDGDGI
                     PDDQDACPDRPEDFDGFEDSDGCPDIDNDMDRVLDIADKCPNVPETYNGYEDEDGCPD
                     TVPPDVDALRGTIEGLLYAEGETVVRDSALSSIRKIARTLAAHPNVRVVLIGHTDDRE
                     AKQLASGDPGQPSDLDALAADLSRARAEAVKNALVAAGIPARRVLVDGKGADEPVADN
                     GKPRGRLANRRVEIKLYIPPR"
     gene            2188..>3370
                     /locus_tag="VF516_15525"
     CDS             2188..>3370
                     /locus_tag="VF516_15525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109143.1"
                     /translation="MLADLLSDRFEIEVQIPGGGMGAVFRGRDRASGAAVAIKVLTDD
                     RAHNTARFAREVELLSELSHPGIVRYIAHGETPSGQLFLVMEWLEGEDLRHRLARGPL
                     AMGEAVTLATRVAAALSAAHARGIVHRDLKPSNLFLPGGHIDQVKVLDFGIAHREGRT
                     QLTQTGAMIGTPGYMAPEQARSNAPIDARVDVFALGCVLFQCLTGIPAFEGDSPVAIL
                     GKILFSEAPRVSALWPHVPEDLDTLVATMLAKDPALRPSDGANLAAALAALGPGMHSS
                     AVAPRERVVLSPAITGGERRLLSVVLLGAAADGEPAEAALEPAIKPYGGRMEQLVDGS
                     TLVVLETDRHVATDQAAQAARCALAMRAITSDRPLGIAVGRAESTQPGLRAGEGIDRA
                     AR"
BASE COUNT          469 a         1266 c         1190 g          445 t
ORIGIN      
        1 cggtcgcgtc gcacgcgtcg ccgagcgcgg cgtggagcgg gcgcgccgcg gccagctccc
       61 gcgcctcgca caggtaccag atcagctcgt ggagctgccg catgatcgcg aacacgtcga
      121 acatccgctg cgcggtgtcc ggtgcgcttc gccagtcctt gccgccgaac gtgacctgag
      181 acacatgctg gccggccccg aaacagtcgt agaccgtgca gcccgggaag ccgagctgcc
      241 ggagccgggg gtggatcgcg cagcgaaagc tcgcctgcag gtgcggacag gcctgccccg
      301 ccggcttgtc gatcgcgaag tccgacgacg ccgcgaacgc cggcgcgacg cagcacagcc
      361 cgaagcagcg cgcacagtcc gcccggaggc cgcggcgatc ggggccccgg ggtggccccg
      421 tggacgcggc gtcggccggc atcgccgaca ggatactcga cgtgtccgcg tccccctgcc
      481 gagcaggatc gaagcgggga atcgaccggt gtgggcgttc atgtagggcg cgcggcctcc
      541 gccatgcata agtccagtct cacgcgacta acctcggccc gtgcctcagg atcgagcggc
      601 tccacggggg ctctcggtcg accatgatgc tgcgatgcgt agactcgaac gcgatgcatc
      661 gcctgtggtg ggtcgcgctg gcgacgatcg cgatcgcgtc gtccgcggcc gcggcagacc
      721 ccaccgagat cggcggctgg tttggcccac gcctgttctc gtcgagctcg gggctcggct
      781 acctcgatga tgcgcccgcc cacccggcgc tgcagaacgg catcgcgttc ggcggccggg
      841 tcgcccgccc gttcctgccc tggctggtcc ccgagctcga gctcgcgctg atgccgacca
      901 gcaccagcgc cgtcggcggc gccagggcag ccagcgtgct gtggttcgaa ccgcggctcc
      961 acctccggtt cgagctgtgg cagggccgcc ggctccagcc gttcgtcctc gccggcggcg
     1021 gctcgccggt gtcgctgtcg accgcgagca agacctacgg cacctcgatc accggcgacg
     1081 gctacctcgg cggcggcgtg cggttcgcca cccagaaggg cttcgacttc cggttcgacg
     1141 cccggatcgc gctcttgccc tcgcagcccg gctccgggtt cgggctgacc cccgagctcg
     1201 acttcggcat cggcatcgag ttccacgtcg gcgagccgcg cccgtccgcg cccgacgagg
     1261 cccggctcgc ccaggccccg ccgcccgacc gcgacggcga cggcatcccc gacgatcagg
     1321 acgcctgccc cgatcgcccc gaggacttcg acggcttcga ggacagcgac ggctgccccg
     1381 acatcgacaa cgacatggat cgcgtgctcg acatcgccga caagtgcccc aacgtgcccg
     1441 agacctacaa cggctacgag gacgaggacg gctgtcccga caccgtgccg cccgacgtcg
     1501 acgcgctgcg cggcacgatc gagggcctgc tctacgccga gggcgagacc gtcgtgcgcg
     1561 actcggcgct gtccagcatc cgcaagatcg ccaggacgct cgccgcccac cccaacgtcc
     1621 gggtcgtgct gatcggccac accgacgatc gcgaggccaa gcagctcgca agcggcgacc
     1681 ccggtcagcc ctcggatctc gatgcgctgg ccgccgacct gtcgcgcgcg cgcgccgagg
     1741 cggtcaagaa cgcgctggtc gccgcgggca tcccggcgcg gcgggtcctg gtcgacggca
     1801 agggcgccga cgagccggtc gccgacaacg gcaagccgcg gggccggctc gccaaccgcc
     1861 gggtcgagat caagctgtac atcccgccgc gatagccggg tggggagcgc ttcgagcacc
     1921 cggccgggct cacgctgatc ctcggcgacc cgcccgacat gcgcgcctca ctcagcgtga
     1981 ctgtaattgt ccggctctcg gacgccttcg gaggagtgcc aggcctgcac gggcgcacgt
     2041 gctgcgattc gcgctgacac cgcgcgacgc agacgacggc gagctcggcc aggtctccgc
     2101 ggagtggcgg gacggctgct cgatcctggg gtaacgtcaa cccgacgtgt catcacccca
     2161 ggcccgggga ggtcggaggg acgcaaggtg ctcgcggatc tgctgagcga tcgattcgag
     2221 atcgaggtcc agatcccggg cggcggcatg ggcgcggtgt tccgcggccg cgatcgcgcc
     2281 tcgggcgcgg ccgtggccat caaggtcctc accgacgacc gtgcgcataa caccgcacgc
     2341 tttgcgcgag aggtcgagct gctctccgag ctgtcgcacc ccggcatcgt ccgctacatc
     2401 gctcacgggg agactccctc gggccagctg ttcctggtca tggagtggct cgagggcgag
     2461 gacctccgcc accgcctcgc gcgcgggccg ctcgccatgg gcgaggcggt cacgctggcg
     2521 acccgcgtcg ccgccgccct cagcgcggcc cacgcgcgcg gcatcgtgca ccgcgatctc
     2581 aagccctcca acctgttcct ccccggcggc cacatcgatc aggtcaaggt gctcgacttc
     2641 ggcatcgcgc accgggaggg ccgcacccag ctgacccaga ccggcgcgat gatcgggacg
     2701 ccgggataca tggcccccga gcaggcgcgc agcaacgcgc cgatcgacgc cagggtcgat
     2761 gtgttcgcgc tcggctgcgt gctgttccag tgtctgaccg ggatcccggc gttcgagggc
     2821 gacagccccg tcgccatcct gggcaagatc ctgttcagcg aggccccgcg ggtgagcgcg
     2881 ctgtggcccc acgtgcccga ggacctggac acgctggtcg ccacgatgct ggccaaggat
     2941 cccgcgctcc ggccgagcga cggggccaac ctggccgcgg cgctggccgc gctcgggccg
     3001 gggatgcaca gctcggccgt ggcgccgcgg gaacgcgtcg tgctctcccc ggcgatcacc
     3061 ggcggcgagc ggcggctgct gtccgtggtg ctgctcggcg ccgccgcgga cggcgagccg
     3121 gccgaggccg cgctcgagcc tgcgatcaag ccgtacggcg gccgcatgga gcagctcgtc
     3181 gatggctcga ccctcgtggt gctcgagacc gaccgccacg tcgccaccga tcaagcggcg
     3241 caggccgcgc gctgcgcgct cgcgatgcgg gcgatcacca gcgaccggcc cctgggcatc
     3301 gcggtcggcc gggccgagtc gacgcagccc gggctgcgcg ccggcgaggg gatcgaccgt
     3361 gccgcgcgcc
//
LOCUS       DASVOR010000785         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_95798,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000785 DASVOR010000000
VERSION     DASVOR010000785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95798"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VF516_15530"
     CDS             <1..594
                     /locus_tag="VF516_15530"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HEX8109144.1"
                     /translation="RIGELGGDPARVAVAGESAGGNLITALALTACQRRDEPWARAVF
                     DTGLVPRAVLPFCAMLEVSRPERFSQRRRLPRWIDGMIHDASASYLHGHPPGPGPATA
                     LADPLRVLEESVAGLPVQRGWDRPLPPFFAPVGTRDPLLDDTRRLARALDGLGVPCEA
                     RYYPGGIHAFHALVWDPAARACWRDALAFLDRHMRKP"
     gene            601..>2008
                     /locus_tag="VF516_15535"
     CDS             601..>2008
                     /locus_tag="VF516_15535"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8109145.1"
                     /translation="MTPPRRQLGFSITAAIVIANMIGTGVFTSTGLQARDLHDPATIL
                     IAWVVGGVLALCGAAAYAELGAMMPQAGGEYVYLSRAYHPAVGFLSGWVSLTAGFSAS
                     IAVSALAFATYLARLVPALDAPAAWLTANLAVGDHHLCTIALGAREAVAIGLIVGITA
                     LHAFDTRLGGWVQAGFTGSKVLLILGFVLCGLAFGRGDWRNLTAQHGGLANLTTPQFA
                     MALMYVSFAYSGWNAAAFVAGEVVRPERTLPRSLLTGAGIVMAVYVLLNLVFLYAVPS
                     DALAGPPGGGPVLEVGDAAARQLFGARAAALVTSVIALALVSGVSAMMIAGPRIYAAM
                     AADGALPEPLSRYSRRGVPAVAVVAQGALGIVFVLVGDLDQLIRFVGFMLTIFAALTV
                     GAVFVLRRRGHRAPYRTFGYPVTPILFIALSGWIAYAQLEEHFAEGLVVLVVLAVGGV
                     LYRVFRRAPRRSTDAAPDA"
BASE COUNT          195 a          755 c          739 g          319 t
ORIGIN      
        1 cgaatcggcg agctcggcgg cgatccggcg cgggtcgcgg tcgccggcga gtcggccggc
       61 ggcaacctga tcaccgcgct cgcgctgacc gcgtgccagc gccgcgacga gccgtgggcc
      121 cgcgcggtgt tcgacaccgg cctggtgccg cgcgcggtgc taccgttctg cgccatgctc
      181 gaggtgtcgc ggcctgagag gttctcgcag cgccggcggc tgccgcgctg gatcgatgga
      241 atgatccacg acgccagcgc gtcgtacctg catggtcatc cgcccgggcc ggggccggcc
      301 accgcgctcg ccgatccgct gcgcgtgctg gaggagtcgg tcgccgggct ccccgtgcag
      361 cgcggctggg accgcccgct gccgccgttc ttcgcgccgg tcggcacgcg cgatccgctg
      421 ctcgacgaca cccggcggct ggcccgcgcg ctcgacgggc tcggcgtgcc gtgcgaggcg
      481 cgctactacc cgggcggcat ccacgcgttc catgccctgg tgtgggatcc ggccgcgcgc
      541 gcgtgctggc gcgacgcgct ggcgttcctc gatcgccaca tgcgcaagcc gtagctcctc
      601 gtgacgccac ctcgccgcca gctcgggttc tcgatcaccg ccgcgatcgt catcgcgaac
      661 atgatcggca ccggcgtgtt caccagcacc gggctgcagg cccgcgacct ccacgatccg
      721 gcgacgatcc tgatcgcgtg ggtggtcggc ggcgtgctcg cgctgtgcgg cgctgcggcc
      781 tacgccgagc tcggcgcgat gatgccccag gccggcggcg agtacgtcta cctcagccgg
      841 gcctaccacc ccgcggtcgg gttcctcagc ggctgggtgt cgctgaccgc cgggttctcg
      901 gcgtcgatcg cggtgtcggc gctcgcgttc gcgacctacc tcgccaggct ggtgcccgcg
      961 ctcgacgcgc cggcggcctg gctgaccgcg aacctcgcgg tcggcgacca ccacctgtgc
     1021 acgatcgcgc tcggcgcgcg cgaggcggtc gcgatcggcc tgatcgtcgg gatcaccgcg
     1081 ctgcacgcgt tcgacacccg gctcggcggc tgggtccagg ccgggttcac cgggtccaag
     1141 gtgctgctga tcctcgggtt cgtcctgtgc ggcctcgcgt tcggccgcgg cgactggcgg
     1201 aatctgacgg cccagcacgg cgggctcgcc aacctcacca ccccgcagtt cgcgatggcg
     1261 ctgatgtacg tcagcttcgc gtactcgggc tggaacgcgg cggccttcgt cgcgggcgag
     1321 gtcgtgcgac ccgagcgcac gctgccgcgc tcgctgctca ccggcgccgg catcgtgatg
     1381 gcggtgtacg tgctgctcaa cctcgtgttc ctctatgcgg tgccgtccga cgcgctcgcc
     1441 ggaccgcccg gcggcggtcc ggtgctcgag gtcggcgacg ccgccgcgcg ccagctgttc
     1501 ggcgcgcgcg cggcggccct cgtgacctcg gtgatcgccc tcgcgctggt gtccggggtc
     1561 agcgcgatga tgatcgcggg cccgcggatc tacgccgcga tggcggccga cggcgcgctg
     1621 cccgagccgc tgagccggta cagccggcgc ggcgtgccgg ccgtcgcggt ggtcgcgcag
     1681 ggcgcgctcg gcatcgtgtt cgtgctggtc ggcgacctcg accagctgat ccggttcgtc
     1741 gggttcatgc tcacgatctt cgcggcgctg accgtcgggg cggtgttcgt gctgcgccgc
     1801 cgcggccacc gcgcgccgta ccgcacgttc ggctacccgg tcaccccgat cctgttcatc
     1861 gcgctgtcgg gctggatcgc ctacgcccag ctcgaggagc acttcgccga gggcctggtc
     1921 gtgctcgtcg tgctcgcggt cggcggcgtg ctgtaccggg tgttccgccg cgcgccgcgg
     1981 cggtcgacgg acgccgcgcc ggacgcgc
//
LOCUS       DASVOR010000786        10022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_300059,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000786 DASVOR010000000
VERSION     DASVOR010000786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300059"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VF516_15540"
     CDS             <1..454
                     /locus_tag="VF516_15540"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEX8109146.1"
                     /translation="FLERLLHRTGIQGDDRRFVDETAHVQAVALVELLATGDPEPLEF
                     RLPLAKLLSGRPLESDFAPDRPITPAQIAETDHLLAAVIDHAPVGELSIPGLRAAFLQ
                     RRAALTTRDGAWLLQVERQPHDAVLDRFPWSWSWIKLPWMPDPLRVEW"
     gene            421..1824
                     /locus_tag="VF516_15545"
     CDS             421..1824
                     /locus_tag="VF516_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8109147.1"
                     /translation="MDARSAAGGVVMPGDVITETLRDLERELAWFVRLLDLRLRSYFP
                     PAGADGDTGPVRALDLAELPPPDHGDSRSPWASFLRDNPLDLVERAALVLAMVPHLRP
                     RLLDVFFTRNATFDRRFTEFGGARLDGEFEPTGETLAFLLGGDALDARLAVARVLEPD
                     RRLIRGDVLRLGTTGRELPAMKAPLRISPEHLSLFTLGRLPRPVLSADFPAQRIETAL
                     DWSDLILHPGTRKQLGEIQAFITHGHTLMRDWNMAARLRPGHRTLFHGPPGTGKTLTA
                     ALLGKTAGRDVYRVDLSLVISKYIGETEKNLARVFDRAQQQDWIVFFDEADALFGKRT
                     ETKDAHDRYANQEVAYLLQRLETFDGVVVLASNMRENLDPAFTRRFESVIYFPLPRPA
                     ERLELWRRGISPRARLDAGVDLAALAEAHELSGGSIMNVIRQVSLAAIADGERPITAD
                     DLQQAIRRELAKDGKRP"
     gene            1836..3704
                     /locus_tag="VF516_15550"
     CDS             1836..3704
                     /locus_tag="VF516_15550"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX8109148.1"
                     /translation="MTARRTAAPHGAPDPSPTTNRASIAAAPPAYGIDLVDAAAGGRG
                     AGASATLPLEQALRRLPALPQNDALGVLKRFQRAIAIRISTGERRIQLAMSQRAESFG
                     SYLIGGTIEVFGLTSLPSEQWEEAWKPLNAAYATIAGREVKASLDLLLAAAAATNRYW
                     EQLNAHLEKTDQGADRSIFALQALEAAGAVAASLLTGGGAAGILVGAGYAGTQNLVGQ
                     ATSMSIGIQDKIDWAGLAFDTVFGAITGVLGGKLGNVVLKRLVGNPAVASVSRRLLAE
                     VVSDLISGRLSSILQTTAHTLFDQLRGREALTMEQFIGHLSDELMDPKAMFLDAILGR
                     AAKLAHASSARPGAPAAHEAAAPREPAPAAHEIPPPAPLHAQEPGGLELDLSGSREGW
                     NKHVEEYQARDEAKLRAETQKPGFGAESSERGGTEHHTGMNFHEYSAAEVAIGLRVDW
                     DPQAGRPRKVTYNFTSAAAKVASQVTERSFHQEPRLKGESAQSREKAYTKSGYDKGHL
                     AQRQAGKVDPAIDAALGLENPTGREVERALDVLTNVAPMTPTLNRGKGSAWASAENRT
                     AQFALQHGYVTVEITPIYDAQPPRLSDGTPIPRQFRRTIKSGPTGNVLEDLVFDNK"
     gene            4204..5793
                     /locus_tag="VF516_15555"
     CDS             4204..5793
                     /locus_tag="VF516_15555"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX8109149.1"
                     /translation="MATPNEKLAASLEELRQLQLDGSRVFASTQLSRTSRERLLKQGF
                     LQEAMKGWLISASPTVQPGDTTPWFASFWEFCRRYCDDHFGNAWHVSPEQSLLLHAED
                     TAIPRQVIIYSPAANNNRIDLLFETSLFALKQKAMPPPGDLETRQGVRVFRIDAALIR
                     VPEGFFADHPIAAQVVLGRIRDPSELLARLLDGGHTVVAGRLAGAFRHLGQPAIADEV
                     VAAMKSAGHAVRETDPLDAERAVAPMPRSAPPIVARLHALWVSARDTVLAALPPPPGR
                     PRDRKAYLRRVDEIYQLDAYHSLSIEGYQVTPDLVQRVASGSRDPELQPLDRDSANAL
                     AARGYWLAFRRARDTVRAILGSAGGVELLRTAHRDWYREMFSPHVAAGLMTAGSLAGY
                     RTHPVYLRGSRHVPPRSDVLRDAMPALFDLIEGEPSPAVRAVLGHWLFGYVHPFPDGN
                     GRIARLLMNALLAGGGYPWTVIRVEHRDEYLAALEAASVAGDIGPFARFIAKQLRAAA
                     VRPPRPRTPRRRATTRRPRRR"
     gene            6026..6691
                     /locus_tag="VF516_15560"
     CDS             6026..6691
                     /locus_tag="VF516_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109150.1"
                     /translation="MPTISRETVLIDRRQPMLRWSAVFAGGACSVGIWLLLELLGVGA
                     GLYAHNAADSAGAMHAASVATRVWSLVAQVIALFFGGMVAGKFAQTYERKLAGLHGLV
                     MWALTSLVGMWATVWVITMITAGVQRAGYGLGTVEISAADTGKALLILGCSMLISLIT
                     AVAGAMAGLRRPDRPADSGGRFSRTEPGYPPPGEPVTTAPPYPTPMTGPATPVIPDDS
                     VPR"
     gene            complement(6693..7820)
                     /locus_tag="VF516_15565"
     CDS             complement(6693..7820)
                     /locus_tag="VF516_15565"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8109151.1"
                     /translation="MPHKTAVILRAADIDRTEIALEQRLNPRSRFRDAWLGHLAGFER
                     VGVSRGRIPPGGESFAYHAHHAEEEWVFILSGRARARIDDREVDLGPGDFAAFPAPQA
                     PHLLTNPFDEDCVYLMGGEARIATDVVRYPDLGKSYALVRGVGPHTAFHALGPAEFPF
                     GPPAQDGTAAPGWRVIGCRGCGSVIAEAALVLAGIAYEREEVDYTEPGPARDRLLAIN
                     PLGQVPTLILPDGSVMTETAAIALHVDELVPAAGLFPAPRDPLRREALRWLMFLIAAV
                     YPTFTYGDDPAKWVGDAGPALRDATHAHRQRLWQQVEAAARGPWFLGPRFSMLDVYVA
                     VMTRWRPRRAWFAEHCGKLSAIAAALDGDPRLAALWAANFA"
     gene            complement(8042..8935)
                     /locus_tag="VF516_15570"
     CDS             complement(8042..8935)
                     /locus_tag="VF516_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEX8109152.1"
                     /translation="MLAIDLTGKHALVAGVADDAGLGFAIAKALGEAGATVSVATWPP
                     ALGIFKTMLERGKLDASLTVRGGGKLQFAKIYPLDAEFDRLDDVPAELRDSRRYRDLG
                     DFTIGGLAAAIAADGGVDIVVHSLANGPEVKKPLLETSRKGYLAAVSASAYSMVSMVS
                     HLGPIMRRGGAFVSLTYMASERVVPGYGGGMSSAKAALESDTRVLAYEAGRAWGHRVN
                     VISAGPYASRAASAIGFIGQMIEYAKANSPLPEAITAADVGATAAFLCSPLASGITGT
                     TIYVDKGYHAMGVAVNRPTLT"
     gene            9037..>10022
                     /locus_tag="VF516_15575"
     CDS             9037..>10022
                     /locus_tag="VF516_15575"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8109153.1"
                     /translation="MRDLLFRGAARAALAPPLVHVLARSRRRGVDRDLDRQVAALLAG
                     QRLLRLPPLDSMDPPHARAYAETALSPLEVAPVAMAEVTDTTVAGRGGPIPVRIFVPH
                     DASPHWIIYLHGGGGVIGSIRSAEPATRWIAARTRCVVASVEYRLGPEHRHPAAIEDA
                     CAAWDALSARVPDGRLAVAGDSFGGYLSAQVDHHTIATGRRRPDLQVLIYPAVDMTQS
                     SPSIDRLADGYLLTRALIQWFRGNYLNPGDDRRAVSPMFWPDHRGAARAIVATAGYDP
                     LVDEGDRYAELLARAGTEVRHRRYPSLIHGFLSLAGRVAAARAAVDELCGDIR"
BASE COUNT         1438 a         3760 c         3428 g         1396 t
ORIGIN      
        1 gttcctcgag cggctgttac accgcaccgg catccagggg gacgatcggc ggttcgtcga
       61 cgagaccgcc cacgtccagg cggtcgcgct cgtcgagctg ctcgccaccg gcgacccgga
      121 gccgctggag ttccgtctgc cgctcgccaa gctgctgtcc ggccggccgc tggagtccga
      181 tttcgcaccg gaccgtccga tcactcccgc gcagatcgcc gagaccgacc acctgctcgc
      241 cgcggtgatc gaccatgcac cggtcggcga gctgtcgatc ccgggcctgc gcgccgcgtt
      301 cctgcagcgc cgcgcggcgc tgaccacccg cgacggcgcc tggctgctcc aggtcgagcg
      361 gcaaccccac gacgcggttc tcgaccgctt cccgtggtcg tggagctgga tcaagctgcc
      421 gtggatgccc gatccgctgc gggtggagtg gtgatgcccg gcgacgtcat caccgagacc
      481 ctgcgcgacc tggagcgcga gctcgcctgg ttcgtccgcc tgctcgatct ccggctgcgc
      541 agctactttc cacccgccgg cgcggacggt gacaccggcc cggttcgcgc gctcgacctc
      601 gccgagctgc cgccgcccga tcacggcgac tcgcgctcgc cgtgggccag cttcctgcgc
      661 gacaacccgc tcgatctcgt cgagcgcgcc gcgctggtcc tcgccatggt cccacacctc
      721 cgcccgcggc tgctcgacgt gttcttcacc cgcaacgcga cgttcgaccg ccggttcacc
      781 gagttcggcg gggcccgcct cgacggcgag ttcgagccga ccggcgagac cctcgcgttc
      841 ctgctcggcg gcgatgcgct cgacgcccgg ctcgcggtcg cgcgcgtgct cgaacccgat
      901 cgccggctga tccgcggcga cgtgctgcgg ctcggcacca ccggccgcga gctgccggcc
      961 atgaaggcac cgctgcggat ctccccggag catctgagcc tgttcacgct cggtcgcctg
     1021 ccgcggccgg tgctgtccgc cgacttcccc gcgcagcgca tcgagaccgc gctcgactgg
     1081 agcgatctga tcctgcaccc cggcacgcgc aagcagctcg gcgagatcca ggcgttcatc
     1141 acccacggcc acacgctgat gcgcgactgg aacatggccg cccggctgcg ccccggccac
     1201 cgcaccctgt tccacggacc gccgggcacc ggcaagaccc tgaccgccgc gctgctcggc
     1261 aagaccgccg gccgcgacgt ctatcgcgtc gatctctcgc tcgtgatctc caagtacatc
     1321 ggggagaccg agaagaacct ggcgcgcgtg ttcgatcgcg cccagcagca ggactggatc
     1381 gtgttcttcg acgaggccga cgcgctgttc ggcaagcgca ccgagaccaa ggacgcccac
     1441 gatcgctacg ccaaccagga ggtcgcgtac ctgctccagc ggctggagac gttcgacggc
     1501 gtcgtggtgc tggcctcgaa catgcgcgag aacctcgacc ccgcgttcac ccgccggttc
     1561 gagtcggtga tctacttccc gctgccgcgg ccggccgagc gcctcgagct gtggcggcgc
     1621 ggcatctcgc cgcgcgcgcg cctcgacgcc ggcgtcgacc tcgccgcgct cgccgaggcc
     1681 cacgagctgt ccggcggctc gatcatgaac gtgatccgcc aggtctcgct ggccgcgatc
     1741 gccgacggcg agcgcccgat caccgccgac gacctccagc aggcgatccg ccgcgagctc
     1801 gccaaggacg gcaagcgccc atgaagcaga ccaggatgac agcccgccgc accgccgcgc
     1861 cgcatggcgc acccgatccg agcccaacga cgaaccgcgc gtcgatcgcg gcggcaccgc
     1921 cggcctacgg gatcgacctc gtggacgcgg cagcgggggg ccgcggcgcc ggggcctccg
     1981 ccaccctgcc gctcgaacag gcgctgcgac ggctccccgc gctgccgcag aacgacgccc
     2041 tcggggtgct caagcgattt cagcgcgcca tcgcgatccg gatctcgaca ggcgaacggc
     2101 ggatccagct ggcgatgtca cagcgcgccg agagcttcgg cagctacctg atcggaggca
     2161 cgatcgaggt gttcggcctc acctcgctgc cgtccgaaca gtgggaggag gcgtggaagc
     2221 cgctgaacgc tgcctatgcc acgatcgccg ggcgcgaggt gaaggcgagc ctggatctcc
     2281 tgctcgccgc cgcggccgcg accaaccgct actgggaaca gctcaacgcc cacctcgaga
     2341 agaccgacca gggcgccgac cgctcgatct tcgccttgca ggcgctcgaa gcggcgggcg
     2401 cggtcgccgc ctcgttgttg accggcggcg gcgccgccgg gatcctcgtc ggcgctggtt
     2461 atgccgggac ccagaacctg gttggccagg ccacctcgat gagcatcggc atccaggaca
     2521 agatcgactg ggccgggctc gccttcgaca ccgtgttcgg cgccatcacc ggtgtgctcg
     2581 gcggaaagct cggcaatgtc gtcctgaaga ggctggtggg caacccggcg gtggcgtcgg
     2641 tgagccgccg cctgctcgcc gaggtcgtct ccgatctgat ctccggccgg ctgtcgtcga
     2701 tcctgcagac gaccgcgcac acgctgttcg accagctgcg cggccgcgag gcgctgacca
     2761 tggagcagtt catcggccat ctgagcgatg agctgatgga ccccaaggcc atgttcctcg
     2821 acgcgattct cggacgcgcc gcgaagctgg cacacgcgtc cagcgccagg ccgggcgcac
     2881 cggcagcgca cgaggctgct gccccgcgcg agcccgcgcc cgcggcgcac gagataccgc
     2941 cccccgcgcc cctgcacgcg caggaacccg gcggactcga gctcgacctc agcggcagcc
     3001 gcgagggctg gaacaagcac gtggaggagt atcaggcgcg agacgaggcc aagctgcgcg
     3061 ccgagaccca gaagcccggg ttcggtgcgg agtcgagcga gcgaggtggc accgagcacc
     3121 acaccggcat gaacttccac gagtacagcg ccgccgaggt cgcgatcgga ctccgcgtcg
     3181 actgggatcc gcaggccggc cggccccgca aggtcaccta caacttcacg tccgccgcgg
     3241 ccaaggtcgc gtcgcaggtc accgagcgca gctttcacca ggagccgcgg ctcaagggag
     3301 aatccgcgca atcccgagag aaggcctaca cgaaatcggg ttacgataaa ggccatctcg
     3361 cccagcggca ggccggcaag gtcgatcctg ccatcgacgc cgcgctcgga ctggagaacc
     3421 cgaccggccg cgaggtggag cgcgcgctcg acgtcctgac caacgtcgcg ccgatgaccc
     3481 cgaccttgaa caggggcaag ggcagtgcct gggcctccgc cgagaaccgg acggcgcagt
     3541 tcgcgctcca gcacggctac gtcaccgtcg agatcacgcc gatctatgac gcccagccgc
     3601 ccaggctcag cgatggcacg ccgatcccca ggcagttccg ccgcaccatc aagagcggcc
     3661 ccacgggcaa cgtgctggag gacctggtgt tcgacaacaa gtgatctcga cggagatgac
     3721 tcgtcgcgct cgcgccgcca gccgtggacg aatccgatcc ggggtttcgc tccgatcgcc
     3781 ggtgtcgacc cgccccaact ccagccccgg caccgcggca acccgcccaa ctccggcccc
     3841 ggcgatgtcg gcgtctgcgt ccgctgcggt cactgcgtcc acgagctgat gctcaacggc
     3901 gcctgctact tctgcggctc caccgacctc gatccggtcg cgatgtcgtc cagaccgccc
     3961 cgggtcatcc cgcccgatca cctgatccgg aagaagccga cctgatcagt gggatgcttg
     4021 ctgaccccgg acaagcatct gtaattaaag gataatccgg aaaccgcagg attcacgtga
     4081 tctggttctg caggattcaa ttgtaatgct acaacatcat ccacgtgtaa ccctgcaaca
     4141 tcttccacgt gtaaccctgc aacatcttcc acgtgtgaca acgcagaccc gtcctgaact
     4201 gccatggcca ctccgaacga aaagctcgct gcctcgctgg aagagctgcg ccagctccag
     4261 ctcgatggat ctcgcgtgtt cgcgtcgacc cagctgtccc gcacctcccg agagcggctc
     4321 ctgaagcaag ggttcctgca ggaggcgatg aagggctggt tgatctcggc gagcccgacc
     4381 gtacagccgg gtgacaccac gccgtggttc gcctcgttct gggagttctg ccggcgatac
     4441 tgcgacgacc atttcggcaa cgcatggcac gtgtcgccgg agcaatcgct gctgctccac
     4501 gcggaggaca ccgccatccc caggcaggtc atcatctaca gcccggccgc gaacaacaac
     4561 cgcatcgatc tgctgttcga gacctcgctg ttcgctctca agcagaaggc gatgccgccg
     4621 ccaggcgatc tggagacccg ccagggggtg cgcgtgttcc gcatcgacgc cgcgctgatc
     4681 cgggtaccgg agggcttctt cgccgaccat cccatcgcgg cccaggtggt gctcggacgg
     4741 atccgcgatc cgtccgagct gctggctcgc ttgctcgacg gcggccacac ggtcgtcgca
     4801 ggccggctcg ccggcgcatt ccgccacctc ggtcagcccg cgatcgccga cgaggtcgtg
     4861 gcggccatga agtccgcggg ccacgccgtt cgcgagaccg atccgctcga cgccgagcgc
     4921 gcggtcgcgc ccatgccgcg atcggcgccg ccgatcgtcg cccggctcca cgcgctgtgg
     4981 gtatcggcac gcgacacggt cctcgcagcg ctgccgcctc cgcccgggcg gccccgcgat
     5041 cgcaaggcat atctgcgccg cgtcgacgag atctaccagc tggatgcata tcactcgctg
     5101 tcgatcgagg ggtaccaggt gacgcccgat ctcgtccagc gcgtcgccag cggcagccgg
     5161 gatcccgagc tccagccgtt ggatcgcgac agcgccaacg ccctcgcggc gcgcggttac
     5221 tggctcgcgt tccgccgcgc ccgcgacacc gtccgcgcca tcctcggctc cgccggcggg
     5281 gtcgagctgc tgcggaccgc ccaccgcgac tggtaccggg agatgttcag cccgcacgtc
     5341 gccgccggcc tgatgaccgc cggttcgctc gcaggctacc ggacgcatcc ggtgtacctg
     5401 cgcggctcgc ggcatgtccc gccgcggtcg gacgtgctcc gggacgcgat gcccgcgctg
     5461 ttcgacctca tcgagggcga gccctcgccc gcggtccgcg ccgtgctcgg ccactggctg
     5521 ttcggatacg tgcatccgtt ccccgatggc aacggccgga tcgcccgctt gctgatgaat
     5581 gcgctgctgg ccggcggtgg ctacccgtgg acggtgatcc gggtcgaaca ccgcgacgag
     5641 tacctcgcgg cgctggaagc ggcgagcgtg gccggcgaca tcgggccgtt cgcgaggttc
     5701 atcgcgaagc agctgcgcgc cgcagcggtg cgcccaccgc ggccgcgcac gccgcgacgc
     5761 cgagcgacga cacgacgccc acggcggcgg tgatcctgcg gacaattccg atcgccgcgc
     5821 aggattgcac tcacgcgaac acgcggatcg cagcgtcgcc gaccgccggt ggcttgcgag
     5881 ttcgccggag ggccgagcgc gaatgcctcg acggtggtgt gcgatcgcga ccaaccccgc
     5941 tgcgcacgcg tgcggccggg cgcgttttca cgagcgcacg tgcggccgac gttgcagcgc
     6001 tcccgtcggg aggtactccc cgatcatgcc caccatcagt cgtgaaaccg tcctgatcga
     6061 tcgcaggcag ccgatgctgc gctggagcgc cgtgttcgcg ggcggcgcgt gcagcgtggg
     6121 catctggctg ctgctggagt tgctcggcgt cggtgccggg ctgtacgcgc acaacgccgc
     6181 ggacagcgcc ggggcgatgc acgcggcgag cgtcgcgacc cgcgtgtggt cgctggtcgc
     6241 gcaggtgatc gcgctgttct tcggcgggat ggtcgccggc aagttcgcgc agacctacga
     6301 gcgcaagctc gccggcctgc acggcctcgt gatgtgggcg ctgacctcgc tggtcgggat
     6361 gtgggccacg gtctgggtga tcacgatgat caccgcgggc gtccagcgcg ccgggtacgg
     6421 cctggggacg gtcgagatct cggccgcgga caccggcaag gcgctgctca tcctcggctg
     6481 ctcgatgctg atcagcctga tcacggcggt ggcgggcgcg atggccgggc tgcgtcggcc
     6541 ggatcgtccc gccgacagcg gcggccggtt ctcgcgcacc gagcccgggt acccgcctcc
     6601 cggcgagccg gtcacgactg caccgccgta cccgacgccg atgaccggcc cggcgacgcc
     6661 ggtgatcccg gacgactcgg tcccgcgctg agtcacgcga agttggcggc ccacagcgcg
     6721 gcgagccgcg gatcgccgtc gagcgcggcc gcgatcgccg acagcttgcc gcagtgctcg
     6781 gcgaaccatg cccgccgcgg ccgccaccgc gtcatcaccg cgacgtagac gtccagcatc
     6841 gagaaccgcg gtccgaggaa ccacggcccg cgcgcggcgg cctcgacctg ctgccacagc
     6901 cgctggcggt gcgcgtgggt cgcgtcgcgc agcgccggcc cggcgtcgcc gacccacttc
     6961 gccgggtcat cgccgtaggt gaacgtcggg tacaccgcgg cgatcaggaa catcagccag
     7021 cgcagcgcct cgcggcgcag cgggtcgcgc ggcgcgggga acagccccgc tgcgggcacc
     7081 agctcgtcga cgtgcagcgc gatcgccgcg gtctcggtca tcacgctgcc gtcgggcagg
     7141 atcagcgtcg gcacctggcc gagcgggttg atcgccagca ggcgatcgcg cgccggcccg
     7201 ggctcggtgt agtcgacctc ctcgcgctcg tatgcgatcc ccgcgagcac cagggcggcc
     7261 tcggcgatca ccgagccgca gccgcggcag ccgatcacgc gccaccccgg cgcggccgtg
     7321 ccgtcctgcg ccggcgggcc gaacgggaac tccgccggtc cgagcgcgtg gaacgccgtg
     7381 tgcggtccga cgccgcggac cagcgcgtag ctcttgccga gatcgggata gcgcacgacg
     7441 tcggtcgcga tccgcgcctc gccgcccatc aggtacacgc agtcctcgtc gaacgggttg
     7501 gtcaacaggt gcggcgcctg cggcgcgggg aacgccgcga agtcgccggg cccgaggtcg
     7561 acctcgcgat cgtcgatccg cgcccgcgcg cgccccgaca ggatgaacac ccactcctcc
     7621 tcggcgtggt gcgcgtggta cgcgaacgac tcgccgcccg gcgggatccg gccgcgcgac
     7681 actccgaccc gctcgaaccc ggcgaggtgc ccgagccacg cgtcgcggaa ccgcgagcgc
     7741 gggttcaggc gttgctccag ggcgatctcg gtccggtcga tgtcggcggc gcgcaggatg
     7801 acggcggtct tgtgaggcat gccccgctat accccgcccg gccgccacgg cgcttgcacg
     7861 cgcttgcgtg cgccgggaaa acgccggccg gatacgacag acgcctgtca ggtttcggcg
     7921 gacgcgacga cgctggccgc gtcggggatc gctgcgctcc cagctgcgat ccaggttgcg
     7981 acgcgggccg acctgccggc gatgatccgg cgcggcccgt gcgacgagca cgccgacggc
     8041 cctaggtcag cgtcgggcga ttgaccgcga cgcccatcgc gtgatagccc ttgtcgacgt
     8101 agatcgtcgt gccggtgatg ccgctggcga gcgggctgca caggaacgcg gcggtcgcgc
     8161 cgacgtccgc ggcggtgatc gcctcgggca gcggcgagtt ggccttggcg tactcgatca
     8221 tctggccgat gaagccgatc gcgctcgcgg cgcgcgacgc gtagggcccg gcggagatca
     8281 cgttgacgcg gtggccccac gcccggccgg cctcgtacgc cagcacgcgg gtgtcggact
     8341 ccagcgcggc cttggccgac gacatgccgc cgccgtagcc cggcacgacg cgctccgagg
     8401 ccatgtaggt cagcgacacg aacgcgccgc cgcgccgcat gatcgggccg aggtgggaga
     8461 ccatcgacac catcgagtac gccgacgcgg acaccgcggc gaggtagccc ttgcgcgagg
     8521 tctcgagcag cggcttcttg acctcggggc cgttggccag gctgtggacg acgatgtcga
     8581 ccccgccgtc ggcggcgatc gcggcggcga gcccgccgat cgtgaagtcg ccgaggtcgc
     8641 ggtagcggcg gctgtcgcgc agctcggcgg ggacgtcgtc gagccggtcg aactcggcgt
     8701 cgagcgggta gatcttcgcg aactggagct tgccgccgcc gcgcacggtg agcgacgcgt
     8761 cgagcttgcc gcgctcgagc atggtcttga agatgccgag cgcgggcggc caggtcgcga
     8821 ccgagaccgt cgcgccggcc tccccgagcg ccttggcgat cgcgaagccg aggccggcgt
     8881 cgtcggccac gccggccacc agcgcgtgct tgcctgtgag gtcgatcgcg agcatgcgac
     8941 ttgatcgcgc gaaacccgcg cggaatcaac cgccgggccg ccaacctgcc gaccgcgcgc
     9001 ccgcgcactc cgccgtgcga tgataccgtc cggcgcgtga gagatctgct gtttcgcggc
     9061 gccgcgcggg ccgcgctcgc ccccccgctc gtccatgtcc tggcgcgcag ccggcggcgc
     9121 ggcgtcgatc gcgacctcga tcgccaggtc gcggccctgc tcgcgggcca gcgcctgctc
     9181 cgcctgccgc cgctcgactc gatggacccg ccgcatgccc gggcgtacgc cgagaccgcg
     9241 ctgtcgccgc tcgaggtcgc gccggtcgcg atggccgagg tcaccgacac caccgtggcc
     9301 ggccgcggcg gcccgatccc ggtgcggatc ttcgtgccgc acgacgcctc gccgcactgg
     9361 atcatctacc tccacggcgg cggcggcgtg atcggctcga tccgcagcgc ggagccggcg
     9421 acccggtgga tcgcggcccg gacccgctgc gtcgtcgcgt cggtcgagta ccggctcggc
     9481 cccgagcacc gccaccccgc cgcgatcgag gacgcgtgcg cggcgtggga cgcgctgtcc
     9541 gcgcgcgtac ccgacggccg gctcgccgtc gccggcgaca gcttcggcgg ctacctctcg
     9601 gcgcaggtcg atcaccacac gatcgccacc ggccgccgcc gcccggatct ccaggtcctg
     9661 atctacccgg cggtcgacat gacgcagagc tcgccgtcga tcgaccggct cgccgacggc
     9721 tacctgctca cccgtgcgct gatccagtgg ttccgcggca actacctgaa cccgggcgac
     9781 gaccgccggg cggtgtcgcc gatgttctgg cccgatcacc gcggcgccgc ccgcgcgatc
     9841 gtcgccaccg cgggctacga cccgctggtc gacgagggcg accgctacgc cgagctgctc
     9901 gcgcgcgccg gcaccgaggt ccgccaccgc cgctacccgt cgctgatcca tggcttcctc
     9961 agcctggccg gcagggtcgc ggccgcgcgc gccgcggtcg acgagctgtg cggcgacatc
    10021 cg
//
LOCUS       DASVOR010000787         4154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_300092,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000787 DASVOR010000000
VERSION     DASVOR010000787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300092"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..290
                     /locus_tag="VF516_15580"
     CDS             <1..290
                     /locus_tag="VF516_15580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109154.1"
                     /translation="CHPAEQRTGLHRVAQHGTCNDCHGAHEPHPADDRATCLRCHTAQ
                     VNHEPRAVRCATCHPFSSGPHASTTPPGRPPIAPPGPRSGNDPAAPRGRRE"
     gene            432..872
                     /locus_tag="VF516_15585"
     CDS             432..872
                     /locus_tag="VF516_15585"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8109155.1"
                     /translation="MNATEARTTLLAQHEQLRLLLDTCSRLARQQRAGEPVTVELDEA
                     LAELRDEFADHNRLETALIAELLQGPAAWSTQMVDRMLEEHVAEHAAFWELLSGTGVE
                     VADRIEELTEELDAHMSAEERTFLSPMTLRDDVIRRRTRELATE"
     gene            complement(903..1481)
                     /locus_tag="VF516_15590"
     CDS             complement(903..1481)
                     /locus_tag="VF516_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8109156.1"
                     /translation="MSNTHAQVVVGYDFSHSGAAALSRAIALAARAPFHVLHVACIVD
                     RHAQLPGHEHHGRVDFAYTERIQQAVIDVIDQELRAAGLEARIQFYVHVRIGKPADEL
                     LGLASDIGADLIIVGSKGLTGVERLVLGSVSEKVVREARCTVEVARPKTYDYVELSHI
                     VEVAPHRTYVPPHRYTYDDPRLEMRPPEWPLY"
     gene            1605..2051
                     /locus_tag="VF516_15595"
     CDS             1605..2051
                     /locus_tag="VF516_15595"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8109157.1"
                     /translation="MSLAPTSRVPPSGQLLDQHHREIEEACLALLGAGYAGEPCELTR
                     RWGEIEHQLYDHMMAEEHFLFPAYQRDEPENAQDLRDQHARLRALALEIGVAVQLHTV
                     RMEQIQAFVAVLRAHGQREEASLYRWADRHLADDERHRMHDYLAAG"
     gene            complement(2053..2673)
                     /locus_tag="VF516_15600"
     CDS             complement(2053..2673)
                     /locus_tag="VF516_15600"
                     /inference="COORDINATES: protein motif:HMM:NF024911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8109158.1"
                     /translation="MASACTCHLSHAPRLVVVTGGPGAGKTALLEVVQRDLCWHAVVL
                     PEAASILWRGQFPRHPTIAGRKAAQRAIVRVQIEMQRMTIEEGRAGLIVCDRGTLDGL
                     AYWPGSEREYFEDIASTKQRELERYATVIHMRTPSLDHGYMPSPFRIESAAEAALIDE
                     QIDAAWAGHPRRFMIDSDDDFVAKLHRALDLIRAEVPACCRARPSS"
     gene            2914..3555
                     /locus_tag="VF516_15605"
     CDS             2914..3555
                     /locus_tag="VF516_15605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109159.1"
                     /translation="MRSFALAILLIGCATTPRPESKGGPRGLRASEHLDAAAQHDAMA
                     RERETWPDMRPAAPGDLRPVSMPWYRSWDTAGEHDRIAAIHRAQAAEIHAQFDEACRD
                     LPEGDARISPLEAYGIGGWNTTTGVIVYLSPDAGPPDRLIARMKCHRAAMLLAPSNME
                     DCPLDLPDIALDARGDEGGVTVSIVVRNLDLVPELQRRAAHDLEAAAQLRSKH"
     gene            3570..>4154
                     /locus_tag="VF516_15610"
     CDS             3570..>4154
                     /locus_tag="VF516_15610"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8109160.1"
                     /translation="MPAYSSEAILRDGASVTIRSLTAADRDHVISVFHRMSPRSVRHR
                     FFTTKRELSPSDLAFLDQLADGPDAALAAIVHTPAGERALGIGRYVVLPDGRSAEVAF
                     EVGDADQGRGIGTLLLEHLAVNARRRGIATFIAEVEGDNRQMMEMFTTSGFAVSNSLD
                     ERTFHVEFPTEDTERFVATAAAREREAAARSLRAV"
BASE COUNT          592 a         1576 c         1435 g          551 t
ORIGIN      
        1 cctgccaccc cgccgagcag cgcaccggcc tgcaccgcgt cgcgcagcac ggcacctgca
       61 acgactgcca cggcgcgcac gagccgcacc ccgccgacga tcgcgcgacc tgcctgcgct
      121 gccacacggc gcaggtcaac cacgagccca gggcggtgcg ctgcgcgacc tgccacccgt
      181 tcagcagcgg tccgcacgcg agcaccacgc cgccggggcg cccgccgatc gcgccgccgg
      241 gtccccgttc cggcaacgac ccggctgccc ctcgagggcg ccgcgagtga gccggcctcc
      301 gagctttccg caccgcatgc ggcgatcctg gcggcgcggc gcaccggctg cgcgaaaccc
      361 ccgtcaagcg gacctaaccc ggtgcaaacc cagcgcggtg cccggcccga cgtttgcgaa
      421 agcccgaggt catgaacgcc accgaagccc gtaccacgct cctggcgcag cacgagcagc
      481 tccgcctcct cctggacacc tgcagccggc tggcccggca gcagcgcgcc ggcgagccgg
      541 tcaccgtcga gctcgacgag gcgctcgccg agctgcgcga cgagttcgcc gaccacaacc
      601 ggctggagac cgcgttgatc gccgagctgc tccagggtcc ggcggcctgg agcacgcaga
      661 tggtcgaccg catgctggag gagcacgtcg cggagcacgc cgcgttctgg gagctgctgt
      721 ccggcacggg cgtcgaggtc gccgaccgga tcgaggagct caccgaggag ctcgacgcgc
      781 acatgtcggc cgaggagcgg acgttcctca gcccgatgac gctgcgcgac gacgtgattc
      841 gccgccggac ccgcgagctc gcgaccgagt agccgcggcc gcggccgggg ctgttgcggt
      901 cgctagtaca gcggccactc cgggggccgc atctcgagcc gggggtcgtc atacgtgtag
      961 cggtggggcg gcacgtacgt gcgatgcggc gcgacctcga cgatgtggct cagctcgacg
     1021 taatcgtagg tcttgggccg ggcgacctcg acggtgcacc gcgcctcgcg caccaccttc
     1081 tcggagaccg agcccagcac gaggcgctcg accccggtca ggcccttgct accgacgatg
     1141 atcaggtcgg cgccgatgtc gctcgccagg ccgagcagct cgtcggccgg cttgccgatc
     1201 cggacgtgga cgtagaactg gatccgggcc tcgaggcccg cggcccgcag ctcctggtcg
     1261 atcacgtcga tcaccgcctg ctggatgcgc tcggtgtagg cgaagtcgac ccgcccgtga
     1321 tgctcgtggc cgggcagctg tgcgtggcgg tcgacgatgc acgcgacgtg gagcacgtgg
     1381 aacggtgccc gggcggccag cgcgatcgcg cgcgacagcg ctgcggcgcc gctgtgactg
     1441 aagtcgtagc cgacgacgac ctgtgcgtgg gtgttgctca tgcggtgatt catcgcaacc
     1501 gacatgccag ccatccgccg gccggaacgc gcaaccaccg cggccgggcg ccggttcgcc
     1561 gcggtctccg gctggacacc ggcttgcaac accggtcgca caccatgtcc ctcgctccga
     1621 cctcccgcgt gccgccgagc ggccagctcc tcgaccagca tcaccgcgag atcgaagagg
     1681 cctgcctcgc gctcctcggc gctgggtatg ccggcgaacc ctgcgagctc acccggcgct
     1741 ggggggagat cgagcaccag ctgtatgacc acatgatggc cgaggaacac ttcctgtttc
     1801 cggcctacca gcgcgacgag cccgagaacg cgcaagatct gcgcgaccag catgcccggc
     1861 tgcgcgcgct cgcgctggag atcggcgtcg cggtccagct ccacaccgtc cgcatggagc
     1921 agatccaggc gttcgtcgcc gtgctgcggg cccacgggca gcgcgaggag gcgtcgctgt
     1981 accgctgggc cgaccgccac ctggccgacg acgagcgcca ccggatgcac gactacctcg
     2041 cagcgggtta ggtcagctcg acggcctcgc ccggcagcac gccgggacct cggcgcggat
     2101 caggtcgagc gcgcggtgca gcttggccac gaagtcgtcg tcgctgtcga tcatgaaccg
     2161 ccgcggatgg cccgcccacg cggcgtcgat ctgctcgtcg atcagcgcgg cctcggcggc
     2221 gctctcgatc cggaacgggc tcggcatgta gccgtgatcg agcgacggcg tccgcatgtg
     2281 gatcacggtg gcgtagcgct cgagctcgcg ctgcttggtc gacgcgatgt cctcgaagta
     2341 ctcgcgctcg gagcccggcc agtaggccag gccgtcgagc gtgccgcgat cgcagacgat
     2401 cagcccggcg cggccctcct cgatcgtcat gcgctgcatc tcgatctgga cccgcacgat
     2461 cgcgcgctgc gccgccttgc ggcccgcgat cgtcgggtgg cgcgggaact ggccgcgcca
     2521 caggatgctg gccgcctcgg gcagcacgac ggcgtgccag cacaggtcgc gctggacgac
     2581 ctcgagcagc gcggtcttgc cggcgcccgg cccgccggtg acgacgacca gccgcggcgc
     2641 gtggctcagg tggcaggtgc aggccgacgc catggctcag cggccctcgc cggtggacgt
     2701 tgtgggcatc accgcacgaa gtgcatcgcg tgttccacgc gcgcgagcga tgtagtcccc
     2761 atggaccacg ggtcctcggc gcggacggcg tccgcaaggt ttgcgggccc acggggtgcg
     2821 cgtgcagcgc ttgcgtcggt gaccgtcgaa gcacgcgcag atcgcgctgg acgctcggcg
     2881 ctgcgcggca cgctccatgc tcggggatcg gtcatgcgct cgttcgccct tgccatcctc
     2941 ctgatcggtt gtgcgaccac cccccgtccc gagtccaagg gcggaccgcg cggcctgcgg
     3001 gccagcgagc acctcgatgc cgccgcccag cacgacgcga tggcccgcga gcgcgagacc
     3061 tggccggaca tgcgcccggc cgcgcccggc gacctgcggc cggtctcgat gccgtggtac
     3121 cgctcgtggg acaccgccgg cgagcacgac cggatcgccg cgatccaccg cgcccaggcc
     3181 gccgagatcc acgcccagtt cgacgaggcg tgccgcgacc tgcccgaggg cgacgcccgg
     3241 atctcgccgc tcgaggccta cggcatcggc ggctggaaca ccaccaccgg cgtgatcgtg
     3301 tacctgtcgc ccgacgccgg tccgcccgac cggctgatcg cgcggatgaa gtgtcaccgc
     3361 gccgcgatgc tgctggcgcc gtcgaacatg gaggattgcc cgctcgacct ccccgacatc
     3421 gcgctcgacg cgcgcggcga cgagggcggc gtcacggtgt cgatcgtcgt gcgcaacctc
     3481 gacctcgtcc ccgagctcca gcgccgcgcg gcgcatgacc tcgaggccgc cgctcagcta
     3541 cgctcgaagc actaggaacc gcgacccaca tgcccgccta cagctccgag gccatcctgc
     3601 gcgatggcgc ctccgtcacg atccgctcgc tcaccgccgc cgaccgcgac cacgtcatct
     3661 ccgtgtttca ccggatgagc ccgaggtcgg tgcggcaccg gttcttcacc accaagcgcg
     3721 agctgtcgcc gtccgacctc gcgttcctcg accagctcgc cgacggcccc gacgccgcgc
     3781 tcgccgcgat cgtccacacg ccggccggcg agcgtgcgct cggcatcggc cgctacgtcg
     3841 tgctgcccga cggccggtcc gccgaggtcg cgttcgaggt cggcgacgcc gatcagggcc
     3901 gcggcatcgg cacgctgctg ctcgagcacc tcgcggtcaa cgcgcgccgg cgcggcatcg
     3961 cgacgttcat cgccgaggtc gagggcgaca accgccagat gatggagatg ttcaccacca
     4021 gcgggttcgc ggtctcgaac agcctggacg agcgcacctt ccacgtcgag ttcccgaccg
     4081 aggacaccga gcggttcgtc gccaccgccg ccgcgcgcga gcgcgaggcc gcggcgcgga
     4141 gcctgcgcgc cgtg
//
LOCUS       DASVOR010000788         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_329125,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000788 DASVOR010000000
VERSION     DASVOR010000788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329125"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..2943)
                     /locus_tag="VF516_15615"
     CDS             complement(37..2943)
                     /locus_tag="VF516_15615"
                     /inference="COORDINATES: protein motif:HMM:NF014868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HEX8109161.1"
                     /translation="MLAPLTDNALAVLRARYLVRDGGVVVETPDQLFRRVAQHVAAAE
                     TAMGYSAAEVAAAQVAFERMMSALEFLPNSPTLMNAGRPLGQLAACFVVPVDDSTTGV
                     FEAVRWAAEIQKTGGGTGFAFSRLRPAGDLVASTGGAASGPVSLMQVFDVATEAIKQG
                     GTRRGANMGILRVDHPDILEFIAIKLDPTRMRNFNLSVAVTDAFMAAVRAGASYALIN
                     PRTGQATTELDARRVLDAIANAAWACGDPGLVFIDRINDAHPTPELGAIEATNPCVTG
                     DTRVWVEDRGLVPIADLVDTTPRIATLDGERVVFRQATQVIATGIRPIYRLTTVEGLE
                     LRLTADHLVTTDRGDVPAEDIEPGDRIRIVSVEAPAALDQDSRDARIGELIGWLTGDG
                     HITNHSGDKRTAVLSFYGRDKREAAPRLLDVARHLISDSSLTLNSVDTRDLVYIRSQR
                     LLHALRELGIDEGCKRCIPEYVWRGSAAVVAGYLRGLFSADGSVQGSTGRGASVRLAA
                     NELGILRGAQQLLLRLGIRSVLYTHRRGEALRLLPDATGVLREYRCAAQHELSIARAS
                     LRTFADAVGFVIHDKTERLRAILDSYGRGPYRDRAWVTVNDIVPEGEAEVFDLTEPTT
                     RHFFANGMLVHNCGEQPLLAFESCTLGSIDVGRFVTTGELDWVRLAAVIRDAVRFLDD
                     VIDANRYPLPEIERATKATRKIGLGLMGWADALAALGLAYDSEAALALAERLAGFLER
                     ESLAASAALAERRGRFPAWPGSRWLRAGHPALRNSTTTTIAPTGTISIIAGCASGIEP
                     LYALAYRRNVLDGAELTEINPHFRRIAAERGFASDALFAAVALHGGVRGRPDVPADVQ
                     RVFPTAHEVDVATHVRMQAAFQRHVHAAVSKTINLRHDATAHDVKAAYQLAYELGCKG
                     ITVYRDGSRDGQVLVTGARSAVVAAAPSCPECGSVLVVQSGCRLCRHCGWSVCG"
     gene            3013..>3227
                     /locus_tag="VF516_15620"
     CDS             3013..>3227
                     /locus_tag="VF516_15620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109162.1"
                     /translation="MILLVLIALVARVDAAPGGASVERAMRYADALTGAGHAAVLDED
                     GDEEAEDRAAERAEIDDRDDEEGIAEGA"
BASE COUNT          470 a         1080 c         1226 g          451 t
ORIGIN      
        1 cggcgggccc gcggattgct gtcgtcgggc ggcatgtcaa ccacagaccg accagccgca
       61 atgccggcac aatcgacatc cactctgaac caccagcacg gagccgcact cggggcagct
      121 cggtgcagca gcgacgacgg cgctgcgcgc gccggtgacc agcacctggc cgtcccggct
      181 gccgtcccgg tacaccgtga tgcccttgca tcccagctcg tacgcgagct ggtaggcggc
      241 cttcacgtcg tgcgccgtcg cgtcgtgccg caggttgatg gtcttgctga ccgcggcgtg
      301 gacgtggcgc tgaaacgcgg cctgcatccg gacgtgggtc gcgacgtcga cctcgtgcgc
      361 ggtggggaac acgcgctgga cgtcggccgg gacgtcgggc cggccgcgga ccccgccgtg
      421 cagggcgacg gcggcgaaca gggcgtcgga cgcgaacccg cgttcggcgg cgatccgccg
      481 gaagtgtgga ttgatctcgg tgagctcggc cccgtcgagg acgttgcgcc ggtaggcgag
      541 cgcgtagagc ggctcgatcc cgctggcgca gccggcgatg atgctgatcg tcccggtcgg
      601 ggcgatcgtc gtggtggtcg agttacgcag cgctgggtgc ccggcgcgca gccaccgcga
      661 accgggccac gcggggaacc tgccgcggcg ctcggcgagc gcggccgagg ctgccagcga
      721 ctcgcgttcg aggaacccgg cgagccgctc ggcgagcgcg agcgcggcct cggagtcgta
      781 ggcgaggccg agcgcggcga gcgcgtcggc ccaccccatg agccccaggc cgatcttgcg
      841 ggtcgccttg gtcgcccgct cgatctcggg cagcgggtag cgattggcgt cgatgacgtc
      901 gtcgaggaac cgcaccgcgt cgcggatcac ggcggccagc cgcacccagt cgagctcgcc
      961 ggtggtgacg aaccggccga cgtcgatcga gccgagcgtg cacgactcga acgcgagcag
     1021 cggctgctcg ccgcaattgt ggacgagcat cccgttcgcg aagaagtggc gggtggtcgg
     1081 ctcggtcagg tcgaacacct cggcctcgcc ttccgggacg atgtcgttca cggtgaccca
     1141 ggctcgatcc cggtacgggc cgcggccgta ggagtccagg atggcgcgca gccgctcggt
     1201 cttgtcgtgg atgacgaatc cgaccgcgtc ggcgaaggtt cgcaggctgg cgcgcgcgat
     1261 cgacagctcg tgctgggcgg cgcagcgata ctcgcgcaag actcccgtcg cgtcgggcaa
     1321 caggcgcagc gcctcgcctc ggcggtgcgt gtagagcacg ctgcggatcc ccagtcgcaa
     1381 caacagctgc tgcgctccgc gcaggatgcc gagctcgttc gcggcgagcc ggacactggc
     1441 gccgcggccc gtcgagccct gcaccgatcc gtctgccgag aacaggccgc ggaggtagcc
     1501 ggcgaccacg gcggcgctac cgcgccagac gtattcggga atgcaccgct tgcagccctc
     1561 atcaatgccc agctctcgca gggcatggag cagccgctgg cttcggatat agaccaggtc
     1621 gcgtgtgtcg acggagttca acgtcagcga cgagtcgctg atgagatggc gggcgacgtc
     1681 gagcaaccgg ggtgctgcct ctcgcttgtc gcgcccgtag aagctcagca ccgcggtgcg
     1741 cttgtcaccg gagtgattcg tgatgtggcc gtcgccggtc agccagccaa tcagctcgcc
     1801 gatccgcgca tccctggaat cctggtcgag agcagccggg gcctcgacgg agacgatgcg
     1861 gatgcggtcg cctggttcga tgtcctctgc ggggacgtcg ccgcggtcag tggtgacgag
     1921 gtggtcggcg gtgagccgga gctcgaggcc ttcgaccgtg gtgagccgat agatcggtcg
     1981 gatcccggtc gcgatcacct gcgttgcctg ccggaacacg acgcgctcgc cgtcgagcgt
     2041 ggcgatgcgc ggggtcgtat cgaccagatc ggcgatcggg acgaggccgc gatcctcgac
     2101 ccacacccgc gtgtcgccgg tgacacaagg attggtcgcc tcgatcgcgc cgagctcggg
     2161 ggtggggtgg gcgtcgttga tccggtcgat gaacaccagg cccgggtcgc cgcacgccca
     2221 cgcggcgttg gcgatcgcgt cgaggacgcg acgcgcgtcg agctcggtgg tggcctggcc
     2281 ggtgcgcggg ttgatcagcg cgtagctcgc gccggcgcgt accgcggcca tgaacgcatc
     2341 ggtcaccgcg accgacaggt tgaagttgcg catccgggtc ggatcgagct tgatcgcgat
     2401 gaactcgagg atgtccgggt ggtcgacgcg caggatgccc atgttggcgc cgcgccgcgt
     2461 accgccctgc ttgatcgcct cggtcgccac atcgaacacc tgcatcagcg acacggggcc
     2521 cgacgcggcg ccgccggtcg aggcgacgag gtcgccggcc gggcgcagcc gggagaacgc
     2581 gaagccggtg ccaccaccgg tcttctggat ctcggcggcc cagcgcacgg cctcgaacac
     2641 gccggtggtg gagtcgtcga ccgggaccac gaagcaggcg gcgagctggc cgagcggccg
     2701 gccggcgttc atcagggtcg gcgagttggg caggaactcg agcgccgaca tcatgcgctc
     2761 gaacgcgacc tgggcggcgg cgacctcggc cgcgctgtag cccatggcgg tctcggcggc
     2821 ggcgacgtgc tgcgcgaccc ggcggaacag ctgatcggga gtctcgacca cgacgccgcc
     2881 gtcgcgcacc aggtagcggg cgcgcaggac cgcgagagcg ttgtcggtga gcggcgcaag
     2941 cacgtgcgca ccgtacgatc cgcggcatgg tcacgcaacc ggccccgacg gcgggtcgcg
     3001 tggctgttga tcgtgatcct gctcgtgctg atcgcgctcg tcgcgcgggt cgacgccgcg
     3061 cccggcggtg cgtcggtcga gcgggcgatg cggtacgcgg acgcgctgac cggcgcgggg
     3121 cacgcggcgg tgctcgacga ggacggcgac gaggaggccg aggaccgcgc ggccgagcga
     3181 gcggagatcg acgaccgcga cgacgaggag gggatcgcgg agggcgc
//
LOCUS       DASVOR010000789         4254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_241424,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000789 DASVOR010000000
VERSION     DASVOR010000789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241424"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /locus_tag="VF516_15625"
     CDS             complement(<1..1028)
                     /locus_tag="VF516_15625"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8109163.1"
                     /translation="MRTKSLVIGVIAAVLPVAAYAQGAGDDPASLQGDDTQWVIPAKN
                     YSATRFSTLSSINPGNVKNLKVAWSMSTGTNQGHEGAPLVVGSTMYVISSYPNIVYAI
                     DMAKDGVIKWKYAPKQDPKAVPVACCDLVHRGMNYSHGKILFSTLDAQVIALDANTGK
                     ELWKAKNGDPSKGETITAAGLIVKDKYIVGISGGEFGVRGHVTAYDINSGKQVWRAYS
                     TGPDEELLLEPDFNAANPHYGQKGQGTSTWQGDAWKRGGGTTWGWYSYDPQLNLFYYS
                     TGNPGTWNPDQRLGDNKWSMTIWARNPDTGKAKWGYQMTPHDAWDYDGVNESILADLT
                     IGGKKVPAL"
     gene            1250..2308
                     /locus_tag="VF516_15630"
     CDS             1250..2308
                     /locus_tag="VF516_15630"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8109164.1"
                     /translation="MRSLGWFATLVVSGGVAGGLAGCKKAPEPAQAPEREERVYVSDE
                     DGGNVVVLSTASDAVVARIPVGKRPRGLRVSPDGTRLFVALSGLPKAGPGVDESKLPP
                     ADPTADGIGIVDLTQLKLLRTISAGKDPELFDVTRDGKRLWVSNEDTAEASLVDVEAG
                     QVVKRAPVGKEPEGVGIHPGGKVVYVTNEADNSVSVLDIESGQPVAQIATGARPRSVV
                     FTADGARAFVSCENAHLLTLIDARSHTRTGDLELPGDGVRPMGLVVSPDGRTLYTSGG
                     RGKTVHVIALDGAAPQVKSTIANVGARPWGIGVTKKGDRLYTANGSAGDVSVIDIATG
                     TVTRHIAVGGAPWGIAIR"
     gene            complement(2335..3216)
                     /gene="fghA"
                     /locus_tag="VF516_15635"
     CDS             complement(2335..3216)
                     /gene="fghA"
                     /locus_tag="VF516_15635"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR02821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEX8109165.1"
                     /translation="MAELVVRSEQRCHGGTIGFYSHASTSTGTEMRFAVYLPPGAETG
                     RLPVLYYLAGLTCTEETFMIKAGALPLAAAAGIILVAPDTSPRGVELPGDREHWDFGI
                     GAGFYVDATESPWAQHYRMYSYVVDELPAIIEARFPAGPRRGIFGHSMGGHGALVVAL
                     RNPARYHSVSAFAPIANPMAVPWGQKAFGRYLGPDRARWEAYDASTLLRRAPSDRDIL
                     VDQGLTDQFLERELHPEALEAAARASGSRVTVRRHAGYDHSYWFIHTFIADHIAWHRE
                     RLHDAGAAPRASRGTAS"
     gene            complement(3216..>4254)
                     /locus_tag="VF516_15640"
     CDS             complement(3216..>4254)
                     /locus_tag="VF516_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013202337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEX8109166.1"
                     /translation="PKAGEVLVELKATGVCHTDEFTRSGADPEGLFPVIFGHEGAGVV
                     VEVGPGVTSVAPGDHVIPLYTPECRGCKSCLSRKTNLCTAIRATQGKGLMPDGTSRFS
                     YKGQKLHHYMGCSTFANHTVLPEIALAKVRKDAPFASICYLGCGVTTGVGAVLWTAKV
                     EAGANVVVFGLGGIGLNVIQGARLVGADKIIGVDINPAREAIGRKFGMTHFVNPKAIQ
                     GDLVAHLVELTGGGADYSFECVGNVGLMRQALECCHRGWGVSVIIGVAGAGQEIATRP
                     FQLVTGRVWKGSAFGGARGRSDVPRLVDWYMDKKIEIDSLITHKLPLDRINEAFDLMH
                     AGASIRTVVEF"
BASE COUNT          622 a         1464 c         1499 g          669 t
ORIGIN      
        1 agcgccggaa ccttcttgcc gccgatcgtg aggtccgcga ggatgctctc gttgacgccg
       61 tcgtagtccc acgcgtcgtg cggcgtcatc tggtagcccc acttcgcctt gccggtgtcg
      121 ggattgcgcg cccagatcgt catcgaccac ttgttgtcgc cgagccgctg gtcggggttc
      181 caggtcccgg ggttgccggt cgagtagtag aacaggttga gctgcggatc gtaggagtac
      241 cagccccagg tcgtgccgcc gccgcgcttc caggcgtcac cctgccacgt gctggtgccc
      301 tggcccttct ggccgtagtg cgggttggcc gcgttgaagt ccggctcgag caggagctcc
      361 tcgtcgggcc ccgtgctgta ggctcgccag acctgcttgc cgctgttgat gtcgtacgcg
      421 gtcacgtggc cgcggacgcc gaactcaccg ccggagatgc cgacgatgta cttgtccttc
      481 acgatcaggc cggcggcggt gatggtctcg cccttgctgg ggtcgccgtt cttggccttc
      541 cacagctcct tgccggtgtt cgcgtcgagc gcgatcacct gggcatcgag cgtggagaac
      601 aggatcttgc catgcgagta gttcatcccg cggtgcacca ggtcgcagca cgcgaccggc
      661 accgccttgg ggtcctgctt cggcgcgtac ttccacttga tgacgccgtc cttcgccatg
      721 tcgatggcgt agacgatgtt ggggtacgag ctgatcacgt acatcgtcga gccgaccacc
      781 agcggcgcgc cctcgtggcc ctggttggtg ccggtcgaca tcgaccacgc gaccttcagg
      841 ttcttcacgt tgcccgggtt gatgctgctc agcgtgctga agcgagtggc tgaatagttc
      901 tttgccggta tgacccactg ggtatcgtcg ccctggagcg acgcggggtc atctccggcc
      961 ccctgggcgt acgctgcgac cggcagcacg gcggcaatga ctccaatgac gagactcttc
     1021 gttctcatgg gtagcctccc atctttcatc acacgctagg aaaaacgaat gacgatgatt
     1081 gcgaaacgga cagtatgcca agaattacag gcatgcaaca tcagatttac aatagattgc
     1141 agattcgtcg ctggtgcgac gtccgcgcgc cggcccgcga tcgaccggcg cagaatgccc
     1201 cggcagctcg gccctggcga agccgcttgt gctgtactac agcttgccca tgcgatctct
     1261 cggttggttc gcgacgctcg ttgtctccgg cggcgtcgcc ggtgggctcg ccggctgcaa
     1321 gaaggcgccg gagccggccc aggccccgga gcgcgaggag cgggtctacg tctcggacga
     1381 ggacggcggc aacgtcgtcg tcctcagcac cgccagcgac gcggtggtcg cgcgcattcc
     1441 ggtcggcaag cggccgcgcg gcctgcgggt atcccccgac ggcacccggc tgttcgtcgc
     1501 gctgtcgggg ctgcccaagg cggggcccgg ggtggacgag agcaagctgc cgccggccga
     1561 ccccaccgcc gacggcatcg gcatcgtcga cctcacgcag ctcaagctgc tgcgcacgat
     1621 ctcggccggc aaggatcccg agctgttcga cgtgacccgc gacggcaagc gcctgtgggt
     1681 ctcgaacgag gacaccgccg aggcgtcgct ggtcgacgtc gaagccggcc aggtcgtgaa
     1741 gcgggcgccg gtcggcaagg agccggaggg cgtcggcatc cacccgggcg gcaaggtcgt
     1801 ctacgtcacc aacgaggccg acaactcggt gtcggtgctc gacatcgaga gcggccagcc
     1861 ggtggcgcag atcgccaccg gcgcccgccc gcgctcggtc gtgttcacgg cggacggcgc
     1921 gcgcgcgttc gtgtcgtgcg agaacgcgca cctgctgacc ctgatcgacg cgcggagcca
     1981 cacccgcacc ggcgacctcg agctgcccgg cgacggggtg cgcccgatgg gcctggtggt
     2041 ctcgccggac ggccgcacgc tctacacctc cggcggccgc ggcaagaccg tgcacgtgat
     2101 cgcgctcgac ggcgcggcgc cgcaggtcaa gagcacgatc gccaacgtcg gcgcgcggcc
     2161 gtgggggatc ggcgtcacga agaagggcga ccggctgtac accgccaacg ggtccgccgg
     2221 ggatgtctcg gtgatcgaca tcgccaccgg cacggtgacg cgccacatcg cggtgggcgg
     2281 ggcgccgtgg ggcatcgcca tccggtgagc caggcgagat cgtgggccgc gcggtcatga
     2341 ggcggttccg cgagaggcgc gcggcgcggc gccggcgtcg tgcaggcgct cgcggtgcca
     2401 cgcgatgtgg tccgcgatga acgtgtggat gaaccagtac gagtggtcgt atccagcgtg
     2461 gcggcgcacg gtcacgcggg acccggacgc gcgggccgcg gcctccagcg cctcggggtg
     2521 cagctcgcgc tcgaggaact gatcggtgag cccctggtcg acgaggatgt cgcggtcgct
     2581 cggcgcgcgc cggagcagcg tgctcgcgtc gtaggcttcc caccgggcgc gatcggggcc
     2641 gaggtagcgg ccgaacgcct tctggcccca cggcacggcc atcgggttgg cgatcggcgc
     2701 gaacgccgac accgagtggt agcgcgccgg gttgcgcagc gcgacgacca gcgcgccgtg
     2761 gccgcccatc gagtgcccga agatgccgcg gcgcgggccc gccgggaacc gcgcctcgat
     2821 gatcgccggc agctcgtcga ccacgtagct gtacatccgg tagtgctgcg cccacggcga
     2881 ctcggtggcg tcgacgtaga accccgcgcc gatcccgaag tcccagtgct cccggtcgcc
     2941 cggcagctcg acgccgcgcg ggctggtgtc gggcgcgacc aggatgatcc cggcggcggc
     3001 ggcgagcggc agcgcgcccg ccttgatcat gaacgtctcc tcggtgcagg tcagcccggc
     3061 caggtaatac agcaccggca gccgcccggt ctcggcgccg ggcggcaggt agaccgcgaa
     3121 ccgcatctcg gtgccggtcg aggtcgaagc gtgggagtaa aagccgatcg tgccgccgtg
     3181 gcagcgctgt tcactgcgga cgacgagctc cgccatcaga actcgaccac cgtccggatc
     3241 gacgcaccgg cgtgcatgag gtcgaacgcc tcgttgatcc ggtcgagcgg cagcttgtgg
     3301 gtgatcagcg agtcgatctc gatcttcttg tccatgtacc agtcgaccag ccggggcacg
     3361 tcgctgcggc cgcgcgcgcc gccgaacgcc gagcccttcc acacccggcc ggtgaccagc
     3421 tggaacggcc gcgtcgcgat ctcctggccg gcgccggcga cgccgatgat cacgctgacg
     3481 ccccagccgc ggtgacagca ctccagcgcc tggcgcatca gcccgacgtt gccgacgcac
     3541 tcgaagctgt agtcggcgcc gccgccggtg agctcgacga ggtgcgcgac gaggtcgccc
     3601 tggatcgcct tggggttgac gaagtgggtc atcccgaact tgcgaccgat cgcctcgcgc
     3661 gcggggttga tgtcgacgcc gatgatcttg tcggcgccga ccagccgcgc gccctggatc
     3721 acgttgagcc cgatgccgcc gagcccgaac accacgacgt tggcgccggc ctcgaccttc
     3781 gcggtccaca gcacggcacc gacgccggtg gtcacgccgc acccgaggta gcagatcgac
     3841 gcgaacggcg cgtccttgcg gaccttggcg agcgcgatct ccgggagcac ggtgtggttg
     3901 gcgaacgtcg agcaccccat gtagtggtgg agcttctggc ccttgtacga gaaccggctg
     3961 gtcccgtcgg gcatgaggcc cttgccctgc gtggcgcgga tcgccgtgca caggttggtc
     4021 ttgcgcgaca ggcaggactt gcagccgcgg cactccggcg tgtagagcgg gatcacgtga
     4081 tcgccgggcg cgaccgacgt gacgcccggg cctacctcca ccaccacccc ggcgccctcg
     4141 tggccgaaga tcaccgggaa caggccttcg ggatcggcgc ccgaccgcgt gaactcgtcg
     4201 gtgtggcaca cgccggtcgc cttgagctcg accagcacct cgccggcctt gggg
//
LOCUS       DASVOR010000790         4772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_300109,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000790 DASVOR010000000
VERSION     DASVOR010000790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300109"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF516_15645"
     CDS             <1..234
                     /locus_tag="VF516_15645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109167.1"
                     /translation="PVRRLLFEVVDLDDPAGLAALCRDDARELPVPGTDRTIAGAPPK
                     RTGVGVTRLGTTAAIALGAYAHALGELGAPADR"
     gene            complement(241..1395)
                     /locus_tag="VF516_15650"
     CDS             complement(241..1395)
                     /locus_tag="VF516_15650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109168.1"
                     /translation="MIDPSTLRVVPSKTARETLRASAVLGAEPQVDGQVTPFAGLLPV
                     WQYTVTAAQDGKTYSGSIVGQAPTSNASTTVPTVIIPVVIRMTQGGTTFVFDPTQPDA
                     GCLGGTNTALALTQGSPLFSNAAFTLNGVSVGTTQWGDANARGEFFSQLAVGSNYHVL
                     LSPTVGPTLTISLNVRNGNSTGEVFSVSGTQCGTNATTNPAARIAVVNINTIDAQLQS
                     YITAHGLTTGQFPFFVTYNAVISNGAANNLNNCCILGYHNALSSGQTYGIGEFEGRNQ
                     TVFTGVSDVAAPSHEVNEWLNDPFGNNPTPAWGGIGQVGGCQTNLEDGDPLSGTLMPA
                     VSLNGFVYHLQELAYFSWFYGGTSLGTGGKFSSNGTFGGAAKLCPPGGTN"
     gene            2119..2436
                     /locus_tag="VF516_15655"
     CDS             2119..2436
                     /locus_tag="VF516_15655"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX8109169.1"
                     /translation="MRWLGMLVLLVASTAHAELSREQVRKTMKAHIREIAACFDTEPP
                     PPDQRVVVAFTIAPSGQVIESVGTGNPPVDDCAAAVIKKVKFPASNSATHVVYPFWLD
                     ATR"
     gene            complement(2568..>4772)
                     /locus_tag="VF516_15660"
     CDS             complement(2568..>4772)
                     /locus_tag="VF516_15660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109170.1"
                     /translation="ASGPQAKLEVSAPGDAAEVEADAIADAATRGGPPTPVHAAPTPV
                     ARHVIHRAALQQGESDKFDASQPGCLVGEAAFGSEVERMLAQPDGHAKLRELLDIPTC
                     YDNARAGLRNYLGRTTDLQIVKKIVRARYDKVLTEEIPWDVPGLKQMYLALDHLPRAH
                     VELNTELTEFQRHRSGPGKVSGGGFYGNKVSSLNYDAISLDAQNTGMGAPGDPLRDVN
                     RFDTAARHEVGHAVDHKGGFSGSYGATSKGGGWKEHPDLDALVDELVAHSPGPIGRLP
                     PSRKQEVVALLKKLVRDGETFEFERKLQAVMAASGWGQPKTVADPDSQNYVTQPDNAS
                     LGTQHVQQALLSDHKARGAVTMFANDDRTTADSIKEDKDALAAAAQLLVNAEGNNTSA
                     EVRTLVESIKLEQYLEGATDPFWQAFAQNHASAASQLLPACGTPSLEALLDAIVPKDN
                     LATKLFQLLQVRAATPEIATMTAEQFRELARDGAHRPRLVDKTKALDELKANGQPVVS
                     HLFEIGDRRDLSDLLTRMVQRDVNVIAPLMALVTSNPKFNQGFNKVSAKADWTKQGAR
                     SNAEGHLRDDARDGTAAYLAAEQDLRNANSGADAQLVDEMKHRTTATAKAAQLGAVSN
                     GETDSFKTAALICNDPAVKALDETVGISPWVKDMPSVIDGRVYHEAYAGRWVSYEAAA
                     RARKVSNYQFRAPGEWFAEAYAAYYEPSQVKGSKLNQRDPDTFTYFQTHVDK"
BASE COUNT          687 a         1615 c         1591 g          879 t
ORIGIN      
        1 ccggttcgcc ggctgctgtt cgaggtcgtc gatctcgacg accccgccgg gctcgccgcg
       61 ctgtgccgcg acgacgcccg cgagctgccg gtgccgggga ccgatcgcac catcgccggc
      121 gcgccgccca agcgcaccgg ggtcggcgtc acccggctcg gtacgaccgc ggcgatcgcg
      181 ctcggcgcct acgcccacgc gctcggtgag ctcggcgccc ccgcggatcg ctgaccgagg
      241 tcagttggtc ccgccgggcg ggcacagctt ggccgcgccc ccgaaggttc cgttgctcga
      301 gaacttgcct cccgttccga gcgaggtacc gccatagaac cacgagaagt aggcgagctc
      361 ctgcaggtga tagacgaacc cgttcaagct cacggcgggc atcaacgtgc cgctcagcgg
      421 gtcaccgtct tccaggttgg tctggcagcc accgacctgt ccgatgccgc cccaggccgg
      481 cgtcggattg ttcccgaatg gatcgttcaa ccactcgttc acctcgtgcg agggcgcggc
      541 gacgtccgag acgcccgtga acaccgtctg attgcggccc tcgaactccc cgattccgta
      601 ggtctgaccg ctactcagtg cattatgata acccagaata cagcagttgt tcaggttgtt
      661 cgccgctccg ttggagatca ccgcattgta cgtcacgaag aaggggaact gtccggtggt
      721 cagcccgtgg gccgtgatgt agctctgcag ctgtgcatcg atcgtattga tgttcacaac
      781 cgcgatcctg gcggccgggt tggtggtggc attggttccg cactgcgtcc cgctgaccga
      841 gaatacctct ccggtgctgt ttccgttcct cacgttgagg ctgatggtca gggtcgggcc
      901 gacggtgggg ctcagcaaca cgtggtagtt gctgcccacc gccagctggc tgaagaactc
      961 gccgcgcgcg ttcgcgtccc cccactgcgt ggtgccgacg ctcacgccgt tgagcgtgaa
     1021 cgcggcgttg ctgaacagcg gcgagccctg cgtcagcgcc agcgcggtgt tcgttcctcc
     1081 gaggcagccg gcgtccggct gcgttggatc gaacacgaac gtcgtcccgc cctgcgtcat
     1141 ccggatcacc accgggatga tcaccgtcgg gacggtcgtc gaggcgttgc tggtgggagc
     1201 ctggccgaca atggagccag aatacgtctt cccatcttgc gccgcggtga cggtgtactg
     1261 ccacaccggc aacaaccccg cgaacggggt cacttgaccg tcgacctggg gctcggcgcc
     1321 aaggaccgcg gatgcgcgaa gcgtctcccg ggcggtcttg ctggggacga cccgcaacgt
     1381 cgacgggtcg atcacccgga gcccgtccgg cccccgttgc tgggcgaacg cgggcgccgc
     1441 gaacagcgca gagcagagaa acgggacgaa gtatctagct ttcatcgttt actatccttc
     1501 tgcaatggac catctcgttt tctcgcccag gagctgcgcg caaactaact cgcccatcac
     1561 gcggtgttcc tccagcgagg ggggtgcagc gatgatcggt ttgttggcgc acgactcctg
     1621 atgagcgatg tcaccctcac tcaatcgagg cggagtcaag acagccgcga attctttgca
     1681 gcggtaacat ggcagtcggc tctgatcaac tgattcttga atgaagcgcg tggctgttcg
     1741 tatccgtgtc gactgtggca tcggtacctg gagcaaatcg ctacacgcgt atcgacatta
     1801 acgggagtat cattttgtcg tcgatccacc ccgcagcgga tcgcgccacc gtcgtggacg
     1861 gcgctcgcat cgtggctgtg taattctcgg acacgcggat gaggttgcgg caacacaact
     1921 ccactgccat cccctgtcac gtatgccggc aaaccaggga ttcgagggac gaaccagaac
     1981 tccgccacgg ccgttctgga gccgttcgaa gccgatcgtt cagggcgtgc gttcgtccgg
     2041 gccgcgcagc accaggagca tcggccgcac gatgcgccgg ggcggccggc ggtcggcgtt
     2101 ccgtgtcatg cttgcgtgat gcggtggctc ggcatgctcg tcctgctcgt ggcgtcgacg
     2161 gcacacgccg agctctcccg ggagcaggtc cgcaagacga tgaaagcgca catccgggag
     2221 atcgccgcct gcttcgacac cgagccgccg ccacccgatc agcgggtggt ggtcgcgttc
     2281 acgatcgcgc cgagcggcca ggtcatcgag tcggtcggga ccggcaaccc gccggtcgac
     2341 gactgcgccg cggccgtgat caagaaggtc aagttcccgg cgtcgaacag cgcgacacac
     2401 gtcgtgtatc cgttctggct cgacgcgact cgatgactgc acgcagctgt cactcgcggc
     2461 gacgcgctcg aggcggccga ggcgctgcac cgtggcggac gaagctgggc ggaggccggg
     2521 gatgcgcctt gcgcacggcg ctgacttcga cgtctggtag ccgcctccta cttgtccacg
     2581 tgggtctgga agtacgtgaa ggtgtcggga tcgcgctggt tcagcttgga gcccttcacc
     2641 tgcgatggct cgtagtaggc cgcgtatgcc tcggcgaacc actcaccggg ggcgcggaac
     2701 tggtagttgg acaccttgcg cgcccgcgcc gccgcctcgt agctcaccca ccggccggcg
     2761 tacgcctcgt ggtacacgcg cccatcgatc accgatggca tgtccttcac ccacggtgaa
     2821 atgccgacgg tctcgtcaag cgccttcacg gcggggtcgt tgcagatcag cgcggcggtc
     2881 ttgaagctgt ccgtttcccc gttgctgacc gcgccgagct gcgcggcctt cgccgtcgcg
     2941 gtcgtgcggt gcttcatctc gtcgaccagc tgggcgtccg cgccggagtt ggcgttgcgc
     3001 agatcctgct cggcggcgag gtaggcggcg gtaccgtcgc gcgcatcgtc ccgcaggtgg
     3061 ccctcggcgt tgctgcgcgc gccctgcttc gtccagtctg ccttcgccga caccttgttg
     3121 aatccctgat tgaacttcgg gttgctggtg acgagcgcca tgagcggcgc gatcacgttc
     3181 acgtcgcgct ggaccatgcg ggtgagcagg tcggagagat cgcgcctgtc gccgatctcg
     3241 aacaggtggg acaccaccgg ctggccgttg gccttcagct cgtccagcgc cttggtcttg
     3301 tcgaccagcc gcgggcggtg cgcgccgtcg cgcgcgagct cgcgaaactg ctcggccgtc
     3361 atggtcgcga tctccggcgt ggcggcgcgt acctgcagca gctggaacag cttcgtcgcc
     3421 aggttgtcct tggggacgat cgcgtcgagc agggcctcca gcgatggggt gccgcacgcg
     3481 ggcagcagct ggctcgccgc gctggcgtgg ttctgcgcga acgcctgcca gaacggatcg
     3541 gtggctcctt ccaggtactg ctccagcttg atgctctcca ccagcgtgcg gacctcggca
     3601 ctcgtgttgt tgccctcggc gttcacgagc agctgcgcgg cggctgcgag cgcgtccttg
     3661 tcctccttga tgctgtccgc ggtggtgcgg tcgtcgttgg cgaacatcgt cactgccccc
     3721 ctcgctttgt gatcgctgag cagcgcctgc tgcacgtgct gcgttccaag cgatgcgttg
     3781 tcgggctgcg tgacgtagtt ctgcgagtct ggatccgcga cggtctttgg ctggccccag
     3841 cccgacgccg ccatcacagc ctgcagcttg cgctcgaact cgaaggtctc gccgtcgcgt
     3901 accagcttct tcaggagcgc caccacctcc tgcttgcgcg acggcggcag gcggccgatc
     3961 ggccccggcg agtgcgccac cagctcgtcg accagcgcgt ccagatcggg atgctccttc
     4021 cagcccccac ccttgctggt cgcaccgtag ctgccggaga agccgccctt gtggtcgacg
     4081 gcgtgaccca cctcgtggcg cgccgcggtg tcgaagcggt tgacgtcgcg cagcggatcg
     4141 ccgggcgcgc ccatgccggt gttctgggcg tccaggctga tcgcgtcgta gttgagcgac
     4201 gaaaccttgt taccatagaa gccgccgccg gacaccttgc cgggaccgga cctgtggcgc
     4261 tggaactccg tcagctccgt gttcagctcg acgtgcgcgc gcggcaggtg atcgagcgcg
     4321 aggtacatct gcttgagccc tggcacatcc caggggatct cctcggtcag caccttgtcg
     4381 tagcgcgcgc ggacgatctt cttcacgatc tgcaggtccg tcgtgcggcc gaggtagttg
     4441 cgcaggccgg cgcgcgcgtt gtcgtagcag gtcgggatgt cgagcagctc gcgcagcttg
     4501 gcgtggccgt ccggctgggc gagcatccgc tccacctcgc tgccgaacgc cgcctcgccg
     4561 acgaggcatc ccggctggct ggcgtcgaac ttgtccgact caccctgctg cagcgccgcg
     4621 cggtggatga catggcgcgc gaccggtgtc ggcgccgcgt gcaccggggt cggcggaccg
     4681 ccgcgggtgg cggcgtcggc aatcgcgtct gcctcgacct ccgccgcatc gccgggcgcg
     4741 ctcacctcca gcttggcctg cggcccgctg gc
//
LOCUS       DASVOR010000791         7018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_270503,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000791 DASVOR010000000
VERSION     DASVOR010000791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7018
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270503"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="VF516_15665"
     CDS             <1..585
                     /locus_tag="VF516_15665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109171.1"
                     /translation="LDFISSRDPNHYYVDNNSEPAVGARLSGTADFDTTGHGVTFGAS
                     VMAGHYDSARQLGFAIGGADIVVNLEGLVLRAEYLVRRTDIVVPPMSTAWTYPLGTPG
                     IPDDHFLKHGFYGEADVPLGAVDLFARFDGLLRFGNTLASSGLSSNARLLRYTAGGSV
                     RISENIRIKASAEYYQSNDLGNDVALHLAVATPF"
     gene            complement(677..1234)
                     /locus_tag="VF516_15670"
     CDS             complement(677..1234)
                     /locus_tag="VF516_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109172.1"
                     /translation="MTGDWAQQFRDIPATLAVRPDLRAAMRRLLDQHELPVKVTEGGD
                     RLAQRRAILGALFDGALTLDQAVAETEARLSRESSPHRTSNLVFATGWAGRLVHTHTS
                     VFYTWAVLDHLLAAGESRCFVPHSPGEAATSTCSRLLAGRDHPADVLRDRLIDVYVHK
                     EPSRAPLVPNHPHCTHVVAPLPGRG"
     gene            1303..2724
                     /gene="rimO"
                     /locus_tag="VF516_15675"
     CDS             1303..2724
                     /gene="rimO"
                     /locus_tag="VF516_15675"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEX8109173.1"
                     /translation="MATRRDAPAAASGQRIYFVSLGCPKNQVDTELMLGQVQAAGHAL
                     VDAPEAADVIVVNTCAFIDAAKEESVDTILEMAQHKQSGARQLVVTGCLAQRYADELA
                     RDIPEIDHLLGSADFPSIAGVLGRAPAPAGRGRAGGKGRGKARLPVIQVAETPSYIYD
                     HDAPRVRIGARHSAYVKIAEGCDRPCAFCIIPKLRGPQRSRAIDDIVAEVRGLARDGV
                     VEVNLIAQDLTRYGWDAGSSPETRATLARLLRALGRVDGVAWIRLHYTFPSAFDDELI
                     DAIASEPRVVKYIDVPLQHISDAMLRRMRRGHSGRVTRELVAKLRARIDGVVLRTTFI
                     VGHPGETAAEFDELCAFVAESKFDRAGAFTYSVEPGTVSALLPHRVDPEVAAERQQTL
                     MEIQRQISRARGEAMVGREIDVLVEGVSSESEYLMEGRWYGQAPGIDGVTYLADGQVP
                     PGSLVRARVTQASDYDLAATLER"
     gene            complement(2754..3476)
                     /locus_tag="VF516_15680"
     CDS             complement(2754..3476)
                     /locus_tag="VF516_15680"
                     /inference="COORDINATES: protein motif:HMM:NF024141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8109174.1"
                     /translation="MTAPAPTPAPGDRGVPPTWLMIRTLAGVTLKRLTRGKALWIAAV
                     LAALPVVFAAVLQGRGRPASPNELLAPSLLLFAVLPALLVGASVGEEIEDRTSTYLWS
                     RPIVRWAVLAGKLIALTPIVVALSVGGWALATALARGVPSIAGCLGLAAGAVATSLVV
                     AGIAVVVPRHGMALTIAYMLVDLFVGELPFSLQQLAVTYHIRVLAGLEGAPAVAAPLI
                     GLAVIAGVWTAIGLSRIRRIEV"
     gene            complement(3473..4414)
                     /locus_tag="VF516_15685"
     CDS             complement(3473..4414)
                     /locus_tag="VF516_15685"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8109175.1"
                     /translation="MGAAAGVVVADRCSKWYGHVLGVSDVSWTLRGGIVGLLGPNGAG
                     KSTLIKMMAGLLQPSRGTLAVYGQSPFHDTAVRRRIGYAPEHEQTWDELTARELVTAM
                     ARLAGVPAGKADATARAALDQVGMTAAMNRKVRGFSKGMRQRTKLATAIAHDPDFLLL
                     DEPLTGVDPMARIDIVARIKQLGEAGKTVVVSSHVLYEIEALTSDILVIYRGQVLAEG
                     NVYAIRQLIDRHPHRIRVECDQPHALAAALAGEDHVARLSFERGAVVIETRDPDRCYD
                     QIAGAVLGAGLAVTALSSPDNNLGAVFEYLTGDGGRR"
     gene            complement(4438..5331)
                     /locus_tag="VF516_15690"
     CDS             complement(4438..5331)
                     /locus_tag="VF516_15690"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8109176.1"
                     /translation="MSAGQIHDLGYKRYVGSRRAVSTRWTVIMRHQIATAWAGWWRFK
                     AWLVAAFLITAVSSVLLYTLGDRLFRRIGGFGGALTRFVDGILPDSTIWYCKAGFLVS
                     LAISATVVAGDLQSGAFTFYFARSMRPRDYVLGKLAGLGVILALIMAAGPLVLAAVRV
                     GLSDNLDQAIDMLPTLYKALAVGALGTLLYTAIPLGFSALIANRRHAMAVWAAYYLLV
                     GWLALGLSLLTTPALAALDLASALHAVSLHLFGLQATGRGADPPTSAALIALLGHAAI
                     AIGLVVWRVRRAQRTGVGGAS"
     gene            complement(5328..6296)
                     /locus_tag="VF516_15695"
     CDS             complement(5328..6296)
                     /locus_tag="VF516_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8109177.1"
                     /translation="MTARIELAGLTKRYGAITALDRVTAAVDGKIIGLLGPNGAGKST
                     LLKCLLGLLPYDGTARVLGLEAARDGAEIRDRVGYMPEQESFLSGMSAVELCTYAGEL
                     SGLPRTEAMQRAHAALYYAGLEEKRYQPIDGYSTGMKQRVKLAQALVHDPEILFLDEP
                     TNGLDPRSREEMLALIVDLPARRGCAIVLSTHLLPDVDRVCDQAVIMHRGAVRFAGTV
                     DELRGARGRDSELFVTVKADAARLAGELTAAGATCRVTSPIALQVDLPASATSQLVFA
                     AARTAGVQVRELGVRRESVEAAFLRVLGEDGAARGVAPAAYPGGAR"
     gene            complement(6293..6751)
                     /locus_tag="VF516_15700"
     CDS             complement(6293..6751)
                     /locus_tag="VF516_15700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEX8109178.1"
                     /translation="MRDASASRVVARCRGSGGADGWLFALAVLVAACGGAPRHEAPPP
                     RTGMATGVQRGLATFYGAEQQGGPTASGERFDRNKLTAAHRTLPLGTRVRVTNTRNGR
                     SVEVRINDRGPYGHGRIIDLSEAAARRLDMIAAGVVPVIVEVLPGAGGGP"
BASE COUNT          978 a         2576 c         2563 g          901 t
ORIGIN      
        1 ctcgacttca tctcgtcgcg cgatcccaac cactactacg tcgacaacaa ctccgagccc
       61 gcggtcggtg cgcggctgtc gggcaccgcc gacttcgaca ccaccggcca cggcgtcacg
      121 ttcggcgcgt cggtcatggc cgggcactac gacagcgcgc ggcagctcgg cttcgcgatc
      181 ggcggcgcgg acatcgtggt caacctcgag ggcctggtgc tgcgcgccga gtacctcgtg
      241 cgccgcaccg acatcgtggt cccccccatg tccacggcgt ggacctaccc gctgggcact
      301 cccggcatcc ccgacgacca cttcctcaag cacgggttct acggcgaggc cgacgtcccg
      361 ctcggcgcgg tcgacctgtt cgcccggttc gacggcctgc tccggttcgg caacacgctc
      421 gcgtcgagcg gcctgtcctc gaacgcccgc ctcttgcgct acaccgccgg cggctcggtc
      481 cggatcagcg agaacatccg gatcaaggcc agcgccgagt actaccagtc caacgacctc
      541 ggcaacgacg tcgccctcca cctcgcggtc gccacgccgt tctgaccgtt gcggctgccg
      601 gtaccgcctc ggcaccctcg cgcagttcga gcgtgtccgc atgcgcatcg ctacgcaatg
      661 taccagaagc cgcggctcag ccccgtccgg gcagcggcgc gacgacgtgg gtgcagtgcg
      721 ggtggttggg gaccagcggc gcgcgcgacg gctccttgtg gacgtagacg tcgatcagcc
      781 ggtcgcgcag cacgtcggcc gggtggtcgc ggccggcgag cagccgcgag cacgtgctgg
      841 tcgcggcctc gcctggcgag tggggcacga agcagcgcga ctcgccggcg gcgagcaggt
      901 ggtcgagcac cgcccaggtg tagaacacgc tggtgtgggt gtggacgagg cgcccggccc
      961 agccggtggc gaacaccagg ttgctggtgc ggtgcggtga gctctcgcgc gacagccggg
     1021 cctcggtctc ggccacggcc tggtcgaggg tcagcgcgcc gtcgaacagc gcgccgagga
     1081 tcgcgcggcg ctgggcgagg cggtcgccgc cctcggtgac cttgaccggc agctcgtgct
     1141 ggtccagcag ccggcgcatc gccgcgcgca ggtcgggccg caccgccagg gtcgccggga
     1201 tgtcgcggaa ctgctgcgcc cagtcgccgg tcacctaccg aagctacccc agaggtttcg
     1261 gattgacagg gccgggtccg cggatcagac tgcccggctt cgatggcgac tcgtcgtgat
     1321 gccccggccg cggcctctgg tcagcgcatc tacttcgtgt cgctcggctg tccgaagaac
     1381 caggtcgaca ccgagctgat gctcggccag gtccaggccg ccggccacgc gctggtcgat
     1441 gcgcccgagg cggcggacgt catcgtggtg aatacctgcg cgttcatcga cgccgccaag
     1501 gaggagagcg tcgacacgat cctggagatg gcgcagcaca agcagtccgg ggcgcgccag
     1561 ctggtggtca ccggctgcct ggcccagcgc tacgccgacg agctcgccag ggacatcccc
     1621 gagatcgacc acctgctggg ctcggcggac ttcccgtcga tcgcgggcgt gctggggcgc
     1681 gcaccggcgc cggccgggcg gggcagggcg gggggaaagg gccgcggcaa ggcgcggctg
     1741 ccggtgatcc aggtcgccga gacgccgagc tacatctacg accacgacgc gccgcgggtc
     1801 cggatcggcg cgcgccacag cgcgtacgtg aagatcgccg agggctgcga ccggccgtgc
     1861 gcgttctgca tcatccccaa gctgcgcggc ccgcagcgct cgcgcgcgat cgacgacatc
     1921 gtcgccgagg tccgcgggct cgcccgagac ggcgtggtcg aggtcaacct gatcgcccag
     1981 gacctgacgc gctacggctg ggacgccggg tcgtcgcccg agacccgcgc cacgctcgcc
     2041 cggctgctgc gcgcgctcgg ccgggtcgac ggcgtggcgt ggatccggct gcactacacg
     2101 ttcccgagcg cgttcgacga cgagctgatc gacgcgatcg cgagcgagcc ccgggtcgtc
     2161 aagtacatcg acgtcccgct ccagcacatc agcgacgcga tgctgaggcg gatgcggcgc
     2221 ggccactccg gccgggtgac gcgcgagctg gtcgccaagc tgcgcgcgcg gatcgacggc
     2281 gtcgtgctgc gcaccacgtt catcgtcggc caccccggcg agaccgcggc ggagttcgac
     2341 gagctgtgcg cgttcgtcgc cgagtcgaag ttcgatcgcg cgggggcgtt cacctactcg
     2401 gtcgagcccg gcacggtgtc ggcgctgctc ccgcaccgcg tcgatccgga ggtcgcggcc
     2461 gagcgccagc agacgctgat ggagatccag cgccagatca gccgggcgcg cggcgaggcg
     2521 atggtcggcc gcgagatcga cgtgctggtc gagggcgtgt cgtcggagtc cgagtacctg
     2581 atggagggcc ggtggtacgg ccaggcgccc gggatcgacg gcgtgaccta cctcgcggac
     2641 ggccaggtgc cacccggctc gctggtccgc gcccgggtca cgcaggccag cgactacgat
     2701 ctcgccgcca cgctcgagcg gtagcgcgcg ggtcgactcg ggctgcgcgc ggctcagacc
     2761 tcgatccggc ggatccggga aaggccgatc gccgtccaca cgccggcgat caccgcgagc
     2821 ccgatcagcg gcgcggcgac tgccggggcg ccctccagcc ccgcgagcac gcggatgtgg
     2881 taggtgaccg cgagctgctg cagcgagaac ggcagctcac cgacgaacag gtcgacgagc
     2941 atgtaggcga tggtcagcgc catgccgtgg cgcggcacga cgaccgcgat cccggcgacg
     3001 accagcgagg tcgcgacggc gccagcggcg aggccgaggc agcccgcgat cgacggcacg
     3061 ccgcgggcga gcgcggtggc gagcgcccag ccgccgacgc tcagcgcgac gacgatcggg
     3121 gtcagcgcga tcagcttgcc ggcgagcacc gcccaccgca cgatcggccg cgaccacagg
     3181 tacgtgctgg tccggtcctc gatctcctcg ccgaccgagg cgccgaccag cagggccggc
     3241 agcaccgcga acagcagcag cgacggtgcg agcagctcgt tcggcgacgc cgggcggccg
     3301 cggccctgga gcaccgcggc gaacaccacc ggcagcgccg cgagcacggc ggcgatccac
     3361 agcgccttgc cgcgggtgag ccgcttcagc gtcaccccgg cgagcgtccg gatcatcagc
     3421 caggtcggtg ggaccccgcg atcgcccggc gcgggcgtcg gcgccggcgc cgtcatcgcc
     3481 gccccccgtc gccggtcagg tactcgaaca ccgcgccgag gttgttgtcg ggcgacgaca
     3541 gcgcggtgac cgcgagcccg gcgccgagca cggcgccggc gatctggtcg tagcagcggt
     3601 ccggatcgcg ggtctcgatc accaccgcgc cgcgctcgaa gctgagccgg gcgacgtggt
     3661 cctcgccggc gagcgcggcg gccagcgcgt gcggctgatc gcactcgacc cggatccggt
     3721 gcgggtggcg atcgatcagc tggcggatcg cgtagacgtt gccctcggcc aggacctggc
     3781 cgcggtagat caccaggatg tccgaggtca gcgcctcgat ctcgtagagc acgtggctcg
     3841 acaccacgac ggtcttgccg gcctcgccga gctgcttgat ccgcgcgacg atgtcgatcc
     3901 gcgccatcgg gtcgacgccg gtcagcggct cgtcgagcag caggaagtca gggtcgtggg
     3961 cgatcgcggt cgcgagcttg gtgcgctggc gcatgccctt ggagaacccg cggaccttgc
     4021 ggttcatcgc ggccgtcatc ccgacctgat cgagcgcggc ccgggcggtg gcgtcggcct
     4081 tgccggccgg cacgccggcg agccgggcca tcgcggtgac cagctcgcgc gcggtcagct
     4141 cgtcccaggt ctgctcgtgc tcgggcgcgt agccgatccg ccgccgcacc gcggtgtcgt
     4201 ggaacgggct ctggccgtag accgccagcg tgccgcgcga cggctggagc aggcccgcca
     4261 tcatcttgat cagggtcgac ttgccggcgc cgttggggcc gagcagcccg acgatgccgc
     4321 cgcgcagcgt ccagctgacg tcggacacgc cgagcacgtg gccgtaccac ttgctgcagc
     4381 ggtcggcgac gaccaccccg gcggccgcgc ccacggctgc gggagatagc gacggcgtca
     4441 cgacgcacct cccacgccgg tgcgctgtgc gcggcggacc cgccacacca ccaggccgat
     4501 cgcgatcgcg gcgtggccga ggagcgcgat cagcgccgcg ctggtcggcg gatcggcgcc
     4561 ccggccggtc gcctggaggc cgaacaggtg gagcgacacc gcgtgcagcg cggacgcgag
     4621 gtcgagcgcg gcgagcgcgg gcgtggtcaa gaggctcagg ccgagcgcga gccagccgac
     4681 cagcaggtag tacgcggccc acaccgccat ggcgtggcgg cggttggcga tcagcgcgga
     4741 gaacccgagc gggatcgcgg tgtagagcag ggtgccgagc gcgccgaccg cgagcgcctt
     4801 gtacagcgtc ggcagcatgt cgatcgcctg gtcgaggttg tccgacagcc cgacccgcac
     4861 cgcggcgagc accagcgggc cggccgccat gatcagcgcc aggatcacgc cgagcccggc
     4921 gagcttgccg agcacgtagt cgcgcggccg catcgagcgc gcgaagtaga acgtgaacgc
     4981 gccgctctgg aggtcgccgg cgaccaccgt cgcgctgatc gccaggctga cgaggaagcc
     5041 ggccttgcag taccagatcg tcgagtccgg gaggatgccg tcgacgaacc gggtcagcgc
     5101 gccgccgaac ccgccgatcc gccggaacag ccggtcgccc agcgtgtaga gcaggaccga
     5161 cgacacggcg gtgatcagga acgccgcgac cagccaggcc ttgaaccgcc accagcccgc
     5221 ccacgcggtc gcgatctggt gccgcatgat cacggtccac cgcgtgctga ccgcgcgccg
     5281 gctgccgacg tagcgcttgt agccgagatc gtggatctgt ccggcgctca tcgcgcacct
     5341 ccggggtagg ccgccggcgc gacgccgcgg gccgctccgt cctcgccgag cacgcggagg
     5401 aacgccgcct cgacgctctc gcgccgcacg ccgagctcgc ggacctgcac accggcggtg
     5461 cgcgccgcgg cgaacaccag ctggctggtg gcgctggccg gcaggtcgac ctggagcgcg
     5521 atcggcgagg tcacccggca tgtcgcaccc gcggcggtca gctcgccggc gagccgcgcc
     5581 gcgtcggcct tgaccgtgac gaacagctcg gagtcccggc cgcgcgcgcc gcgcagctcg
     5641 tcgaccgtcc ctgcgaaccg caccgcgccg cggtgcatga tcaccgcctg atcgcacacc
     5701 cggtcgacgt cgggcaggag gtgggtcgac agcacgatcg cgcagccgcg ccgcgccggc
     5761 agatcgacga tcagcgcgag catctcttcg cgcgaccgcg ggtcgagccc gttggtcggc
     5821 tcgtcgagga acaggatctc ggggtcgtgg accagcgcct gggccagctt cacgcgctgc
     5881 ttcatgccgg tcgagtagcc gtcgatcggc tggtagcgct tctcctcgag cccggcgtag
     5941 tacagcgcgg cgtgggcccg ctgcatcgcc tcggtgcgcg gcaggccgga cagctcgccg
     6001 gcgtaggtgc acagctcgac cgcgctcatc ccggacagga acgactcctg ctccggcatg
     6061 tagccgaccc ggtcgcggat ctcggcgccg tcgcgggcgg cctcgaggcc gagcacgcgc
     6121 gcggtcccgt cgtagggcag caggccgagc aggcacttga gcagggtcga cttgcccgcg
     6181 ccgttggggc cgagcaggcc gatgatcttg ccgtcgaccg ccgcggtgac gcggtcgagc
     6241 gcggtgatcg cgccgtagcg cttggtcagc ccggcgagct cgatccgcgc ggtcatggtc
     6301 cgcctcccgc gccgggcagc acctcgacga tcaccggcac cacgccggcg gcgatcatgt
     6361 cgagcctccg ggcggcggcc tccgacagat cgatgatgcg gccgtggcca tagggtccgc
     6421 ggtcattgat tcgcacctcc accgagcgtc cgttgcgcgt gttggtcacc cgcacgcggg
     6481 tcccgagcgg cagggtccgg tgcgcggcgg tgagcttgtt gcggtcgaac cgctcgcccg
     6541 acgcggtcgg gccgccctgc tgctcggcgc cgtagaacgt cgcgagcccg cgctggaccc
     6601 ccgtcgccat gccggtgcgc gggggcggtg cctcgtgccg cggggcgccg ccgcaggccg
     6661 ccaccagcac cgccagcgcg aacagccagc catccgcacc gcccgatccg cgacatcgtg
     6721 cgacgacgcg ggacgcggac gcatccctca cgagcgccac gcggctcctc gccccgatcg
     6781 cgcgccaggt ccgcgattcc agcgaacatc acgcggcgag ccgcgcgatg gaacgcgcag
     6841 gtgcccccgt cctcgatccg gcatctcggc aatgtagcag ctcggccttg cacatcgcgt
     6901 gctcgcgccg gctgcgccct cagcgggtgc agccggcccc gagcgtccgg ccggccttgt
     6961 cgagcgccgc cttgcggagc tgggcgccgg tcggtccatc gccgagcttc ttgcggcg
//
LOCUS       DASVOR010000792         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_300127,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000792 DASVOR010000000
VERSION     DASVOR010000792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300127"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VF516_15705"
     CDS             complement(<1..523)
                     /locus_tag="VF516_15705"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEX8109179.1"
                     /translation="MKTALLFPGQGSQRVGMGRDLAHEFAVARQTYEEADDVLGFALS
                     RLCFEGPEDELTLTKHTQPAILTTSIAVFRALAERGLAFDVVAGHSLGEWTALVAAGS
                     IAFRDAVRLTHLRGQYMQDAVPAGQGAMAAVMGLDRAALLAVCERARAPGEPVEQANL
                     NGAGQIVISGSAAA"
     gene            676..1287
                     /locus_tag="VF516_15710"
     CDS             676..1287
                     /locus_tag="VF516_15710"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8109180.1"
                     /translation="MTMKIFGHPMSTCTRKVLMTLAETNTPYEFVLVDFAKGEHKQAP
                     HTQRQPFGQVPAMQDGDFELYESRAMCRYINEKVNGPLIPRDLRDRARMEQWISIETS
                     NFTGPTMKFVFHHIFQRHQTPETLETAAGQLNTALGIMDKQLAAHPFIAGGSFTLADV
                     SFMPYIEYAMNTPAKEHFAKHAHVMTWWNKISERPTWRKVAGR"
     gene            1695..>2973
                     /locus_tag="VF516_15715"
     CDS             1695..>2973
                     /locus_tag="VF516_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8109181.1"
                     /translation="MASGVAMMVATNLCSLGIVTFMGRGIQAIKDARPDDVPRDVALL
                     IAFALATAITRILSRVWIFNAARAAEYDLRSDLFGHLMTLSPGYYRDHPTGDVMSRLT
                     NDVQTVRAMWGAGAVHLANAVTAFATVLTLMVMLDPKVAVLSILPYPLIFFTGQALSR
                     RVYRTTREVQAELGTLSSRIQEDLGAIQVIKTYGLEDVRRKGFLAGSQRLLDKNMAAA
                     KVRVQLGPILNTLAPLGVAILILVGGRAIIHNEITVGYFTEFSVLLARLVWPTLTLGF
                     MLALVQRGRASWSRLVELQQTRTDIPDGDGPPAAASAQPARVEVKDLSIQIGGRAILD
                     RVSFELAPGSITAIVGRTGSGKSTLVEALCRLIAVPPGAIFLDGRDITAIPLASLRGQ
                     IGYAPQEAFLFSTTIADNIAMGYGGGTAIPAARA"
BASE COUNT          473 a         1085 c          966 g          449 t
ORIGIN      
        1 ccgccgccgc gctgccgctg atcacgatct ggcccgcgcc gttgaggttg gcctgctcga
       61 ccggctcgcc cggtgcgcgg gcgcgctcgc acaccgccag gagcgcggcg cggtcgagcc
      121 ccatcacggc cgccatcgcg ccctgcccgg ccggcaccgc gtcctgcatg tactggccgc
      181 gcaggtgggt cagccgcacg gcgtcgcgga acgcgatcga ccccgcggcg accagcgcgg
      241 tccactcgcc gagcgagtgg ccggcgacga cgtcgaacgc aaggccgcgc tccgcaagcg
      301 cccggaacac cgcgatcgag gtggtgagga tcgccggctg cgtgtgcttg gtgagcgtca
      361 gctcatcttc ggggccctcg aagcacagcc gcgacagcgc gaaccccagt acatcgtcgg
      421 cctcctcgta ggtctgccgc gcgacggcga actcgtgcgc gagatcgcgg cccatgccga
      481 cccgttgcga gccctggccg ggaaacagca gcgcggtctt catcgccggc tatgtcgcac
      541 ggcgaggcgg cgtggggaag tgactcaccc gacgtttcga tcgcgcggcg cgttgtcgcg
      601 ccgccacaca gcgcacgcac gatcgtcacc cccgaaccta cgaacctacc gcagaaccgt
      661 gctaggagcg ggcccatgac gatgaagatc ttcggccacc cgatgtccac ctgcacgcgc
      721 aaggtcctca tgacgcttgc cgagaccaac accccctacg agttcgtgct cgtcgacttc
      781 gccaagggcg agcacaagca ggcgccgcac acccagcgcc agccgttcgg ccaggtcccg
      841 gcgatgcagg acggcgactt cgagctctac gagtcgcgcg cgatgtgccg ctacatcaac
      901 gagaaggtca acggaccgct gatcccgcgc gacctgcgcg accgcgcgcg gatggagcag
      961 tggatcagca tcgagacctc gaacttcacc ggcccgacga tgaagttcgt gttccaccac
     1021 atcttccagc gccaccagac cccggagacg ctcgagaccg ccgccggtca gctcaacacc
     1081 gcgctcggga tcatggacaa gcagctcgcc gcgcatccgt tcatcgccgg cggcagcttc
     1141 acgctcgccg acgtctcgtt catgccgtac atcgagtacg cgatgaacac gccggccaag
     1201 gagcacttcg ccaagcacgc gcacgtgatg acgtggtgga acaagatcag cgagcggccg
     1261 acctggcgca aggtcgccgg ccgctgagtc gatgcaaggg accgaggtcg atcccggaca
     1321 ttcgaccttg tcctcgacga tgaccttgac ccgaacgttg acgtcgacgg caacgtagtc
     1381 gtctgccccc cacccttgac cttgcaggtt gactccggag gaccgtcggc acggagcgga
     1441 ggtcgacgtc gaggccgacg tcaaaccgtc aacgtcacca ccgcttgcct cccggatcgc
     1501 cctccctttc gttcgaccac ccgcgcaccg gacagatccc ctgtaattca tcaattccac
     1561 taaggaaatc ggctccggct atatgcctcc ccggtcggtg agtgcgcaga tcgccgattg
     1621 ggtgatagac aaggcactcc cacgtggccg gtgcgcgcag ctccctgggt tacctgaggc
     1681 cgtaccggcg ggcggtggcc agcggggtcg ccatgatggt ggcgaccaac ctgtgctcgc
     1741 tcggcatcgt gaccttcatg ggccgcggca tccaggcgat caaggatgcg cgtcccgacg
     1801 acgtgccgcg cgacgtcgcg ctcctgatcg cgttcgcgct cgcgacggcg atcacccgga
     1861 tcctgtcgcg ggtctggatc ttcaacgccg cgcgcgctgc cgagtacgac ctgcgcagcg
     1921 atctgttcgg ccacctgatg acgctgtccc ccggctacta ccgggaccac ccgaccggcg
     1981 acgtgatgtc gcggctgacc aacgacgtcc agaccgtgcg tgcgatgtgg ggcgcgggcg
     2041 cggtccacct cgccaacgcg gtgaccgcgt tcgccacggt gctgacgctg atggtgatgc
     2101 tcgatccgaa ggtcgccgtg ctgtcgatcc tcccctaccc gctgatcttc ttcaccgggc
     2161 aggcgctgtc gcggcgggtc taccggacca cgcgcgaggt ccaggccgag ctcggcacgc
     2221 tgtcgtcgcg gatccaggag gacctcggcg cgatccaggt gatcaagacc tacggcctgg
     2281 aggacgtgcg acgcaagggc ttcctcgctg gctcgcagcg cctgctcgac aagaacatgg
     2341 cggcggccaa ggtccgcgtc cagctcggcc cgatcctcaa cacgctggcg ccgctcggcg
     2401 tcgcgatcct gatcctggtc ggcggtcgcg cgatcatcca caacgagatc accgtcggct
     2461 acttcaccga gttctcggtc ctgctcgcgc gcctggtgtg gcccacgctg acgctcggct
     2521 tcatgctcgc cctggtccag cgcggccgcg cgtcgtggag ccggctggtc gagctccagc
     2581 agacccggac cgacatcccc gacggcgacg ggccgccggc ggccgcgagc gcgcagccgg
     2641 cgcgggtcga ggtcaaggac ctcagcatcc agatcggcgg ccgcgcgatc ctcgatcgcg
     2701 tgtcgttcga gctggcgccg ggcagcatca ccgcgatcgt cggccgcacc ggctcgggca
     2761 agagcacgct ggtcgaggcg ctgtgccggc tgatcgcggt gccgccgggc gcgatcttcc
     2821 tcgatggtcg cgacatcacg gcgatcccgc tggcgtcgct gcgcggccag atcggctacg
     2881 cgccccagga ggcgttcctg ttctcgacca cgatcgccga caacatcgcg atgggctacg
     2941 gcggtggcac cgcgatcccg gcggcccgcg ccc
//
LOCUS       DASVOR010000793         3435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_358441,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000793 DASVOR010000000
VERSION     DASVOR010000793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3435
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358441"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VF516_15720"
     CDS             complement(<1..478)
                     /locus_tag="VF516_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109182.1"
                     /translation="MFSAAGFAVPPTRILACGIWLVGFAGCYNPATLEEGAPCQRSEQ
                     CPEPQQCVLGRCSLHAPPPPDARPATPPDAMVDAMPVPCVADGLSCASGTAPSVFQCG
                     THCWVKCAEMMARATAETRCNNWMGALGEIDDATEQTCVASHISTATFWIGAIQSNP"
     gene            complement(619..897)
                     /locus_tag="VF516_15725"
     CDS             complement(619..897)
                     /locus_tag="VF516_15725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109183.1"
                     /translation="METSEAQRIAAVRYGSLQRLAIAPRRGSHRVISDGAAASPNMSA
                     MDDAAACTNRIFLEGHHIQALGRRWRDKPPKYGLAVLLEAARPIGVRE"
     gene            927..2261
                     /locus_tag="VF516_15730"
     CDS             927..2261
                     /locus_tag="VF516_15730"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8109184.1"
                     /translation="MASTAPASSRWHTGLTSTHWRVLTASFLGWIFDGYETYALIAVL
                     PAALRSLLTPEQLKTPAVWAGTAIGATLLGWGIGGLVGGTLADYFGRKRVMIASVLLY
                     AGLSGLTALSPSFSALIALRFTTGMAMGSEWSTGVAMVAETWPNRARAKGAGFLQSGF
                     GWGTLLAAVIWWVVSRWSPLGDQSWRLMFVIGAVPALFTLYIRRAMAESESWLKAVRE
                     RRWAATEAEPGVARAERRPFTVTEIFREPESRRRVLLMFVLSLTSIVGWYAISTWLPA
                     HVQSLAVHEGQPHPDEWAARAGILYTAGAVMAYVSSGFVIDAIGRRRFLVLTYAGALV
                     LTPVTYFWVTSAQAILAVAAINGFFTLGLAYSWLAIYPPELFTPSVRSTAASLVFNGT
                     RLIAWVFPILAGKLIPHLGGIPRTAMTFGVIYVLGLIVPWFLPETKGKLLPE"
     gene            complement(2289..2789)
                     /locus_tag="VF516_15735"
     CDS             complement(2289..2789)
                     /locus_tag="VF516_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8109185.1"
                     /translation="MSSSTDPSAPATPGAAPPRPFRVLGLQQIAIGGPSKAALRRLWV
                     DLFGLPITGTFRSERENVDEDILAIAGVEVDLMQPIDPQGKPKVHEPPLNHIGLWVDD
                     LPAAVAWLTAQGMRFTPGGIRRGAAGHDVCFIHPRGDATAPLSGEGVLIELVQAPPDV
                     IARLGA"
     gene            complement(2828..3244)
                     /locus_tag="VF516_15740"
     CDS             complement(2828..3244)
                     /locus_tag="VF516_15740"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HEX8109186.1"
                     /translation="MRALIILCALATVARAQPKPPPPSPDDPIGDKLFPPDRIMGHQQ
                     ELGLDDATRKTIVAQIQQFQAAAVKIQWDLKAAGDALANLLAAPAVDEAKALAEADKV
                     MTLEHDLKKAHLTLLIRLKNALTPTQQARLRGLRGH"
     gene            complement(3241..>3435)
                     /locus_tag="VF516_15745"
     CDS             complement(3241..>3435)
                     /locus_tag="VF516_15745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109187.1"
                     /translation="VAAAAALVGVAGVVLRPAPGAPELAQGGVGMSSTSLRLPLDSLL
                     AVPGEDLLASTPSFDQGALP"
BASE COUNT          457 a         1187 c         1246 g          545 t
ORIGIN      
        1 ccgggttgct ctggatcgcg ccgatccaga acgtggccgt gctgatgtgc gaggccacgc
       61 aggtctgctc ggtcgcgtca tcgatctcgc ccagcgcgcc catccagttg ttgcacctcg
      121 tctcggccgt cgcgcgcgcc atcatctcgg cgcacttcac ccagcaatgg gtgccgcact
      181 ggaacaccga gggggccgtg ccactcgcac agctcaggcc gtcggcgacg cacggcaccg
      241 gcatcgcgtc gaccatcgca tcgggtggcg tggccgggcg cgcatcgggc ggaggcggcg
      301 cgtgcagcga gcagcgaccg agcacgcact gctggggctc cgggcactgc tccgaccgct
      361 gacacggcgc cccctcctcc agcgtcgccg ggttgtaaca gccggcgaac ccgaccagcc
      421 agatcccaca ggcgaggatc cgggtcggcg gcaccgcgaa gccggctgca gaaaacatct
      481 gtcgtacatc ctattacgtc tgacgcgccg gcggttgttc ccatccgtgg tggtgcgggg
      541 caccgatcgt gaggcccgac caggcatcga gccatggccg cggcatccct cgatggcagc
      601 cgaacgctgt ggtccaggtc actctcgaac gccgatcgga cgggcggcct cgaggagcac
      661 ggcaaggccg tattttggag gcttgtctcg ccaccgtctg cccagtgctt ggatgtggtg
      721 accctccagg aagatccggt ttgtgcacgc cgccgcatca tccatcgcgg acatgttcgg
      781 cgatgccgcc gcgccatcgc tgatcacgcg atgggaaccc cttcgaggtg cgatcgcgag
      841 acgctggagc gatccgtacc gcaccgccgc gatgcgctgc gcctcggacg tctccacccg
      901 cccgggagac gattaccatg cgcggcatgg cgtccaccgc acctgcctcg agccgctggc
      961 acaccggcct gacgagcacc cactggcgcg tcctgacggc gagcttcctg ggctggatct
     1021 tcgacggcta cgagacctac gcgctcatcg cggtgctgcc cgcggcgctg cggagcctgc
     1081 tgacccccga gcagctgaag acgcccgccg tctgggccgg cacggcgatc ggcgcgacgc
     1141 tgctcggctg gggcatcggc gggctcgtcg gtggaacgct cgcggactac ttcggtcgca
     1201 agcgcgtgat gatcgcctcg gtcttgctct atgcgggact gtccgggctc accgcgctga
     1261 gcccgagctt ctccgcgctg atcgcgctgc ggttcacgac cgggatggcg atgggcagcg
     1321 agtggagcac cggcgtcgcg atggtcgccg agacctggcc caaccgggcg cgcgccaagg
     1381 gggccggctt cctgcagtcc gggttcgggt gggggacgct gctcgccgcc gtgatctggt
     1441 gggtggtgag ccggtggagc ccgctggggg atcagtcgtg gcggttgatg ttcgtgatcg
     1501 gggcggtccc ggcgctgttc acgctgtaca tccggcgcgc catggcggag tccgagtcgt
     1561 ggctcaaggc cgtgcgcgag cgccggtggg ccgcgacgga agccgaaccg ggcgtcgccc
     1621 gcgccgagcg ccggccgttc acggtcaccg agatctttcg cgagccggag agccgccggc
     1681 gcgtcctgct catgttcgtc ctgtcactga cctcgatcgt cggctggtac gcgatctcca
     1741 cctggctgcc cgcccatgtc cagagcctcg ccgtccatga aggccagccg catcccgacg
     1801 agtgggccgc cagggcgggc atcctgtaca cggccggtgc cgtgatggcc tacgtgtcct
     1861 cgggctttgt gatcgacgcc atcggccgcc ggaggttcct ggtgctgacg tacgcgggcg
     1921 cgctggtgct gacgccggtg acgtacttct gggtcacgtc cgcgcaggcg atcctggccg
     1981 tggcggcgat caacgggttc ttcacgctgg gactggcgta ctcgtggctg gcgatctatc
     2041 cgcccgagct gttcacgccg agcgtgcgct cgacggcggc gagcctcgtg ttcaacggga
     2101 ctcgcctgat cgcctgggta tttcccatcc tggcggggaa gctgatcccg catctgggcg
     2161 ggattccacg gaccgccatg acgttcgggg tcatctacgt gctcgggctc atcgtcccgt
     2221 ggttcctccc ggagacgaag ggcaagctgc tgccggaatg atcgcgggac ggccgcgagg
     2281 agtcgcggct acgccccgag ccgcgcgatc acgtcgggcg gcgcctggac cagctcgatc
     2341 agcacgccct cgccggacag cggcgcggtg gcgtcgcccc gggggtggat gaagcagacg
     2401 tcgtggccgg cggcgccgcg ccggatcccg cccggcgtga accgcatgcc ctgcgcggtg
     2461 agccaggcga ccgcggcagg caggtcgtcg acccacaggc cgatgtggtt gagcggcggc
     2521 tcgtggacct tcggcttgcc ctgcggatcg atcggctgca tcaggtcgac ctcgacgccg
     2581 gcgatcgcga ggatgtcctc gtcgacgttc tcgcgctcgc tgcgaaatgt cccggtgatc
     2641 ggcaggccga acaggtcgac ccacagccgg cgcagcgcgg ccttgctcgg cccgccgatc
     2701 gcgatctgct gcaggccgag cacgcggaac ggccgcggcg gggcggcgcc gggggtggca
     2761 ggggcggatg ggtcggtgga gctcgacacg gtggcgcgag cttacgccag acggcgcgcc
     2821 ccggccgtca gtgaccgcgc agcccgcgca gccgcgcctg ctgcgtcggg gtcagcgcgt
     2881 tcttgagccg gatcagcagg gtgaggtgcg ccttcttgag gtcgtgctcc agcgtcatca
     2941 ccttgtcggc ctcggcgagc gccttcgctt cgtcgaccgc gggtgcggcg agcaggttcg
     3001 cgagcgcgtc gcccgccgcc ttgaggtccc actggatctt cacggccgcc gcctggaact
     3061 gctggatctg ggcgacgatc gtcttgcggg tcgcgtcgtc gagcccgagc tcttgctggt
     3121 ggcccatgat ccgatccggc gggaacagct tgtcgccgat cggatcgtcg ggcgatggcg
     3181 gcggcggctt gggctgcgcg cgggcgacgg tcgcgagcgc gcacaggatg atgagtgctc
     3241 tcatggcagg gctccttgat cgaacgacgg tgtcgacgcg agcagatcct cgccgggcac
     3301 ggcgagcagc gaatcgagcg gcaggcgcag cgacgtcgag ctcatgccga cgccgccctg
     3361 cgcgagctcg ggcgccccgg gagccggccg cagcacgacc ccggcgacgc cgaccagggc
     3421 ggccgcggcc gcgac
//
LOCUS       DASVOR010000794        18309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_124546,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000794 DASVOR010000000
VERSION     DASVOR010000794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 18309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18309
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124546"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..7396
                     /locus_tag="VF516_15750"
     CDS             113..7396
                     /locus_tag="VF516_15750"
                     /inference="COORDINATES: protein motif:HMM:NF036757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HEX8109188.1"
                     /translation="MLDAGHRGAHSIHAVGPEQFAAQLAPAGVTTDEANAMFARADQV
                     HATSLTLLANFNSAFTGATPAAVAAPRITSPAITALASLPTVQSLFNTADYCACSDCR
                     SVHGPAAYFVDILEFLKSRMAPDGTTSARDVVLGRSTPTVILGRRPDLKFIQLSCSNT
                     NGVVPYVDLVCEILEDAVGAAPTDNDLKARQTSGSPEELRANPAFVRDTAYTTLSSAQ
                     FPITAPFDLFGVTVRAFLRQLGITRNDLMTAFQSSSSPTDAQIAAERFGLNATALNVI
                     TTASPSAPWTLWGLQQSNNSVPDPRKPDDTTAVRVGSNLQIMAFVPILLDRAKLSHRE
                     LIQLLEARFVNPNIPASGGNPAKALWIVETGVQIGTQTNVASCDTGLQSVTGWTDDLL
                     TGFNRFIRLWRQLGCTIWDLDKVLMAPQLCNGVLDATAIAQLGKLDVIARRLNLPWDE
                     LLTLWGPIDSVDYINVLDPEEPVVRSVYKRKFRSATIAQVATVFTDDPGALTSNLDDP
                     NVIAGISAALAISADDLKRIRAAAGLTTPSGGSAPALNLTNLSKITRYVTLASGLGLS
                     IADLLTAITVSGVNPFPGSLTPAATLQFFTAIDQIQSAGFTLPELNYLLLHGAVVDSK
                     IGLLDTTITAWLEDVRKALVRLGSTDAATAADVVVRRLGALLPLDLTLTQQVLQAKLP
                     GSSQTMASLFTDPALTARNTDGSFTTPTARGNFAAIYDACTAIDKMRIVLARWNVSPV
                     DALWLLKNAHTASWMELHTLPAHAGATTPATLDAFDALHKNVVVQQTLASLAGVRLFD
                     FVLAPGGTGAGTLATAVTNVAQLGGWADADINALATRFNWTTPGQLVAGATVPRIRDL
                     MAWPRKLGTDVTTTLVFAGATVGSSEASKARQLTKSRFSNDEWLGVAAAIQDRLREQK
                     RAALVDYLLANPQSARNQKWVTVEDLYGFFLIDPQMSPVADTTRIKQAVASLQLFIQR
                     CFLQLELFNVDSDSVWKQWDWMKRFRMWEANRKIFLYPENWYDPAQRRDMSPYFQALV
                     NDLQQTNLTNDVAEDALRGYLHKLVDVAHLEVSGLWEEPVQGSAQSILHVVARTRKTP
                     YVYSYRRRETTGGWSAWEPLDDGTNADHIMPVIWNKRLFMLWTEFTEKPQPVADADHA
                     VPSSAAGTLVPLPKKYWELALSWIERRNNIWLPKRQSQRKQIINGVFSGSTNQVFKWQ
                     VAFRATTPLPTGAPTGTTLSNLALSLYEPTGPSSQALAQWILTSPQDEPQLLNTDLTT
                     LATVAVEDMKYVAALPPGTAQIGARPKFPNTAGSPVDMNYVFNAFGGDAPNVTGSLGL
                     QRPVGSIPLVTILNKITMARVISVRYGDINQFSSPFFISDPQRTFFGTFTTSSNWKLQ
                     TFYHPYADAFVQQLNQGGIPGLYDRALQVNPDAARGLTAAFDFNATYTPNTDTTILAT
                     PYPTETIDYTQSGPYAIYNWELFLHTPLLIAKRLADNQRFDEALRWFHYIFNPNTVNV
                     PGSSSPGPVPARYWNPKVFRDLVSADYTAQQIEQVLALVDQGVADAVQRVSDWRNNPF
                     DPNLVAAARPVAYQKAVVMAYISTLIAWGDQLFRGDTVESINEATQLYLLASQLLGPR
                     PQSLRAIQPKQTSNYQDLGTSIDAFANVMVNIENIVSLAPPTTTGSPPTPTMLHTFYF
                     CIPPNDQMLSCWDTVADRLFKIRHGMNLDGVARPLSLFEAPIDPGLLARAAAAGIDIS
                     AALADAAASVGCYRFQTLWQAAHDLCQDVRGLGSAILSATERKDAEEMARLKATQEVA
                     VTTAVREVKAKQLEEARASKTALLSTRAMAEIRKAYYEDPARRNLMNDAETQAQDLNT
                     QAQHNEEAAQDHDRNASRTAWIPSITVGVSGFGGSPTLHTTFGVENYRAHVAALTAVL
                     RDKAGINSRKAGQSTTFASYQRRKDEWDLQLALVTQELDQLDKQLIAADLRIAIAENE
                     LAVQDRQIADAQDTAALLQSKFTSQELYTWMVSQLSSTYFQAYQLAYDLAKRASKAYS
                     FELGQPDPGFIQFGYWDSLHQGLVAGDKLLLDLRRLQAAHLNNNTRELELTKHVSLLQ
                     LDPEQLVRLRETGSCDFILPESLFDLDQPGHFYRRLKTVSVTLPCVTGPYHGVNATLT
                     LVGHSIRTSTNASGYGTTTTDNGLPSDPARFTYVASDNRSIAISTGREDAGMFEVNLR
                     DERYLPFEAAGAISRWHLDLQQQNNRFDLATLSDVVLHARYTARDGGGALRQAASDAV
                     SANPPPGARVQLLSARTEFPDAYARLFAPTGSGQRLDLALAAQHFPFVPASQQITITG
                     VLAILTFTNDKNYTDYANLTPGLRLTARLGFTPGDGTLPTASKPFAPETTHLSGVPAT
                     DAVALSGPIGPVTIGFVESELVNATTLLVQPETQPDSTVLHRLIRDKIDDLLIVINFQ
                     VGPKT"
     gene            7518..15191
                     /locus_tag="VF516_15755"
     CDS             7518..15191
                     /locus_tag="VF516_15755"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEX8109189.1"
                     /translation="MTDNKDRYGSGDRAARRADSGSSGSGSTTSTARDGRFSAPALSL
                     PKGGGAIRGIGEKFAAHPATGTGKLSIPLPLSPGRAGFTPALSLDYDSGAGNGPYGFG
                     WKVDYPAITRRTDQGLPQYRDDRESDVFLISGAEDLVPVTKGDGSRDELVRNGYRVTR
                     YRPRIEGLFARIERWASLTDRADVFWRSISRDNVTTTYGKTAASRIADPADSTKIFTW
                     LVCESFDDKGNASTYEYIAEDSRNVNRGLASEAHRSDASRGTQRYLKRVKYGNRTSRL
                     VQPDLSTMEWLFELVMDYGDHSGEAPSVAPDQDWAVRPDPCSMARAGFEVRTYRRCRR
                     ILMFHRFAELGISPRLVRSLELDYDDFTYPPGFDVRTELAWQGSTRTASLLRRVTSFG
                     HADGGLRTSLPPLELTYSRPQIVEDVQTLADDSYANLPVGVDDRQASWLDLDGEGLSG
                     VLSEYEGAWYYKPNLGNGRLGSVKPVAQRPSIARRGRTKFLDLDGDGALDLVELDHPR
                     AGFHERGERGQWSAFRPFISQPDVTFDDPNLRLIDLTGDGRADVLITQDDQLCWHPSL
                     GEQGFGPRVALRMPADEAQGPRVVFEDGTETIFLADMSGDGLSDIVRVRRSQICYWPN
                     LGYGRFGRRVTMDSSPVLDSQERFDPRRVRLADIDGSGAVDLIYLGADAVRIYFNRAG
                     NGWTAPFQLRAFPEFDHTSNVQVADVLGNGTACLVWSSPLAHSAPVPLRFVDLMGGQK
                     PHLLVGIENNFGARTVIKYTASTQFYLQDQQAGRPWITRLPFPVHVVERVEIYDDISR
                     NRFVTRYAYHHGFYDGVEREFRGFGMVEQIDTEEIAALAADQQLPPATNIDQSSQVPP
                     VLTRTWFHTGVFIGADRISRFFAGLLDGTDRGEYYREPGLTDAQAAQLLLDDTVLPPG
                     LTPDEAREACRALKGMMLRQEIYALDGTDQQPHPYAVTEQNFTLRLVQPKGANRHPVL
                     FSHPRESIVYHYERVPSDPRVAHTLTLEVDGFGNTLKTATVGYGRRTADPALSADDQA
                     QQAAIHITYLDNQVTNAIQASDDYRVPLPSEAKTYELTGLALAAGASRFTLDQLINAG
                     TSAAEIAYEQTPTASVVEKRLIECLRTLYRRNDLTAAAPFGTLESLALPYETYRLAFT
                     PGLVTTVYGTRITDATLSTDGGYVHSESDARWWVPSGRVSFSASDVTPAAELTTAQQH
                     FFLARRYRDPFGEASAVTFDAYDLLVAETSDALGNRITVGERDSAGNLVSAGHDYRVL
                     QPRVITDPNANRSAVSFDALGMVVGMAVMGKRDDSPRRGDLLDGFVPDLTDDVVAAHL
                     ADPLSNPLAILASATVRTVYDRFAYYRTKQTAQPAPTVVYTLTRETHDADLSPGQQTK
                     TQHRFAYSDGFGREIQHKAQAEPGSVPVRDGNGQIIVVNGVPQLTTTAVSPRWVGSGW
                     TVFNNKGKPVRQFEPFFTDRSSFEFDVRIGVSPIVCYDPIGRVVATVQANHAWEKVSV
                     GAWQQVTWDVNDTALIADPREDLDVGAMLGRLDSNDLLPTWYAQRQGGDLGSDEQDAA
                     TKTAAHAATPTIANFNSLGRTFLTIAHNRFKPSDAPPGDPPVEEHYVSRVVLDIEGNA
                     RRMLDAAGRTVVQYDYDMLGHRLHQASMEAGEHWKLEDVLGKPIHSHDSRGHHTRTEY
                     DALRRPLRTFVLGADPQRPTTELLVVKTDYGEGQPSDVQLNLRTRAYRQYDGAGIVMN
                     DAFDFKGNPLQTHRQLVTDYKAVADWSTTPALQQDSFASSTTYDALDRPLSVTTPDAS
                     IVRPSFNEANLIERVDVQLQGAQTTTAFVANIDYDARGQRTRLVLGNNVTTDYAYDRL
                     TFRLKRLVTTRSTDQATLQDLGYTYDAVGNVTRIHDAAQQTIYFNNQVVTPDSSYVYD
                     AVYRLIHTDAREHIGQSLPTQTTWNDQFYINLPHPGDGQAMRRYGEDYRYDAVGNLLQ
                     LVHQASGGSWTRSYTYQETSQLEPGAASNRLSRTAVGSDPPDLYTHDVHGNMTAMPHL
                     AAMQWDYQDRLSTTSRQVVNSGTPETTYYVYASTGERVRKVTERQNGTRKSERIYLGG
                     FEIYRAYDASGATVTLERDTLHVMDDHRRIALVDTRVVGTESGVPQQLVRYQLDNQLG
                     SATLELDDQAQIISYEEYYAYGSTSYQAGRSVAEVSLKRYRYTGMERDEETGFAYHGA
                     RYYAPWLGRWLSADPSGVDNDGPNLYAYVHNNPITYVDPSGLEGEEPQKGQFQWAKTK
                     EENDARDERLQGLFEYLKVHGVGAGSLEKHLEKHGDLEILKHYGYEKPSVLDSPNADN
                     RREMNQRAVMRAIYNYHYEHIGTPQTEAKPLTQANVDAHNAAVAEGQRIESWTNTTLA
                     GAHTSVIGAGVAEVTGHFTDDPKKIDAGRRTGSLVGDVGLTLSPVIAQRLAALRALSA
                     ASQAPVNGSGGSIKSVGGGTSSPGSPLTLVGSGGSGSGLQGRLVLVTSWASRGITPDL
                     RPGRWVQLGGPTWLNFLKTGLIGPKAYLSGTFPYLRIAPSNVPFSNSISGLVPPSSLS
                     WPPGVEAWKGLLGQRILKP"
     gene            15188..15517
                     /locus_tag="VF516_15760"
     CDS             15188..15517
                     /locus_tag="VF516_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109190.1"
                     /translation="MTYIAFIVTGLFLMKILWNVTVPYTLAVRSFRAKDGESRGISLL
                     PFVELALLGVAAGLSLTERGSPWSWSIGTTVLVGGAIAVASYVHLIIAGAIAGWVVKK
                     LRARRGR"
     gene            15563..15898
                     /locus_tag="VF516_15765"
     CDS             15563..15898
                     /locus_tag="VF516_15765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109191.1"
                     /translation="MMIDETAAQNALAGLFRAGVGHWNILTGVAQAMSVAGKQRLDIV
                     DFFWRLMNASPAVLDEEQLNLVDDFTGALLGNCPLDRIIRLHGDPEDPQALGELVVAD
                     AARWKPPGS"
     gene            complement(15923..16582)
                     /locus_tag="VF516_15770"
     CDS             complement(15923..16582)
                     /locus_tag="VF516_15770"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8109192.1"
                     /translation="MALLATRASKNPADWSDRELLRCVLRNESRAWSELIRRYRPLIY
                     RCITKVTLKYAPSLSAADLDEIYADVMMQLVRDDMHKLRIYNPARGTKLGSWIGMISV
                     NAAYDFLRSAGRRPLLDKVDGMLDPHEECDRTPLDQLIEKERWEHLNSLLSDFTDKDR
                     TFVELYYQRGMEADEIAVEMQISLKTVYSKKHKIRAHLVRCLQGLAAAGDSPIADLAV
                     A"
     gene            complement(16914..>18309)
                     /gene="glpK"
                     /locus_tag="VF516_15775"
     CDS             complement(16914..>18309)
                     /gene="glpK"
                     /locus_tag="VF516_15775"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEX8109193.1"
                     /translation="GRGYQEFRQIYPEPGWVEHDPEDIWASVCSALQAALQGVDPASV
                     AAIGITNQRETTVVWDRATGQAVHRAIVWQDRRTAARCAELTAAGKGPRVRELTGLTL
                     DPYFSGTKLAWLLHHVPGAAAAASAGELAFGTIDSYLVWRLTGGAIHATDVSNASRTL
                     LFDLHQLAWSDEMLELIGAPRAVLPGVVESSGTLGVTRGVPGIRDGVPIAGLAGDQQS
                     ALFGQACFTPGDAKCTYGTGAFILMNTGDRPIASHSGLLTTVAWKLATGELRYALEGS
                     AFIAGAAVQWLRDGLQLFASAADVEALAASVPDSGGVIVVPAFAGLGAPHWRPEARGL
                     ITGLTRGATRGHIARATLEGIALQNVDILRAMERDAGRPLTVLKVDGGASANDLLMQF
                     QSDVLGVEISRPDLVETTALGAAFLAGLGTGVWKDQAQVQKTWREQKRFTPAADRARV
                     AEHLARWDAAVRKA"
BASE COUNT         3136 a         6420 c         6001 g         2752 t
ORIGIN      
        1 gatcggcagc gacgcgctgc ccgcgggcga ggaccgcgcc gaggtcatcg ccgacctgaa
       61 gacgctgcag cgcgcctaca ggctgacctc gcgcttcgac cacgccaagg cgatgctcga
      121 cgccggtcat cgcggcgcgc actcgatcca cgcggtcggc cccgagcagt tcgccgcgca
      181 gctggcgccg gccggggtga ccaccgacga ggccaacgcg atgttcgccc gggccgacca
      241 ggtgcacgcg acctcgctca cgctgctggc caacttcaac agcgcgttca ccggcgcgac
      301 gcccgccgcg gtcgccgcgc cgcgcatcac cagtccggcg atcacggcgc tcgccagcct
      361 ccccacggtc cagtcgctgt tcaacacggc ggactactgc gcgtgctcgg actgccgctc
      421 ggtccacggc ccggcggcct acttcgtcga catcctggag ttcctgaagt cgcggatggc
      481 gccggacggc acgacgagcg cgcgcgacgt cgtgctgggt cgctccaccc ccaccgtcat
      541 cctggggcgc cgtcccgacc tcaagttcat ccagctgtcg tgcagcaaca ccaacggcgt
      601 cgtcccgtac gtcgacctgg tgtgcgagat cctcgaggac gcggtcggcg cggccccgac
      661 cgacaacgac ctcaaggcgc gccagacctc ggggagcccc gaggagctgc gggccaaccc
      721 ggcgttcgtc cgggacaccg cgtacacgac gctgtccagc gcgcagtttc cgatcacggc
      781 gccgttcgat ctgttcggcg tcacggtccg ggcgttcctc cgccagctcg gcatcacccg
      841 gaacgacctg atgacggcgt tccagagttc atcgagcccg accgatgccc agatcgcggc
      901 cgagcggttc gggctcaacg cgaccgcgct gaacgtcatc accaccgcga gcccgtcggc
      961 gccgtggacg ctgtggggtc tgcagcagag caacaactcg gtccccgatc cgcgcaagcc
     1021 cgacgatacg accgccgtcc gcgtcggcag caacctgcag atcatggcgt tcgtgccgat
     1081 cctgctcgac cgcgcgaagc tgtcgcaccg cgagctgatc cagctgctgg aggcgcggtt
     1141 cgtcaacccg aacatcccgg ccagcggggg caacccggcc aaggcgctct ggatcgtgga
     1201 gaccggcgtc cagatcggta ctcagaccaa cgtcgcctcg tgcgataccg gcctgcagag
     1261 cgtcacgggc tggaccgacg atctactgac cgggttcaac cgcttcatcc ggctgtggcg
     1321 ccagctcggc tgcacgatct gggacctcga caaggtgctg atggcgccgc agctctgcaa
     1381 cggcgtgctc gacgccacgg cgatcgccca gctcggcaag ctcgacgtga tcgccaggcg
     1441 gctgaacctg ccgtgggacg agctgttgac gctgtggggg ccgatcgaca gcgtcgatta
     1501 catcaacgtg ctcgaccccg aggaaccggt cgtgcgctcg gtctacaagc ggaagttccg
     1561 cagcgccacc atcgcgcagg tcgcgacggt gttcaccgac gatcccggcg cgctgaccag
     1621 caacctcgat gatccgaacg tgatcgccgg gatctcggcc gcgctcgcga tctcggccga
     1681 cgacctcaag cggatccgtg ccgccgccgg gctgacgacc ccgtccggcg ggagtgcgcc
     1741 cgcgctcaac ctgacgaacc tgagcaagat cacgcggtac gtgaccctcg ccagcggcct
     1801 cggcctctcg atcgccgatc tgctgacggc gatcacggtc agcggggtga acccgttccc
     1861 gggctcgctc acgccggccg cgacgctcca gttcttcacc gcgatcgatc agatccagag
     1921 cgccggcttc acgctgccgg agctgaacta tctgctgctc cacggcgcgg tggtcgacag
     1981 caagatcggc ctgctcgaca ccacgatcac ggcgtggctg gaggacgtgc gcaaggccct
     2041 ggtccggctc ggcagcacgg atgccgcgac cgccgcggac gtcgtggtgc ggcggctcgg
     2101 cgccctgctc ccgctcgatc tgacgctcac ccagcaggtc ctgcaggcca agctgcccgg
     2161 ctcctcgcag accatggcgt cgctgttcac ggatcccgcg ctcaccgccc gtaacacgga
     2221 tggctcgttc accacgccca ccgcgcgcgg caacttcgct gcgatctacg acgcctgcac
     2281 cgcgatcgac aagatgcgca tcgtgctggc gcgctggaac gtgagcccgg tcgacgccct
     2341 gtggctgctg aagaacgcgc acacggccag ctggatggag cttcacacgc tgcccgccca
     2401 cgccggcgcg accacgccgg cgacgctcga tgcgttcgat gcgctgcaca agaacgtggt
     2461 cgtgcagcag acgctggcga gcctcgccgg ggtgcggctg ttcgacttcg tcctcgcgcc
     2521 cggaggcaca ggcgccggca cgctcgcgac cgcggtcacc aacgtggccc agctcggcgg
     2581 ctgggccgat gcggacatca acgcgctggc gacccggttc aactggacca ccccgggtca
     2641 gctggtggcc ggcgccacgg tgccccggat ccgcgacctc atggcctggc cgcgcaagct
     2701 cggcaccgac gtcaccacga ccctggtgtt cgccggggcc acggtcggca gcagcgaggc
     2761 gagcaaggcg cgccagctga ccaagtcccg gttcagcaac gacgaatggc tcggcgtcgc
     2821 cgctgcgatc caggatcgcc tgcgcgagca gaagcgcgcc gcgctggtcg actacctgct
     2881 ggcaaatccg cagtccgcgc gcaatcagaa gtgggtcacg gtcgaggatc tgtatggctt
     2941 cttcctgatc gatccgcaga tgtcgccggt cgcggacacc acgcggatca agcaggcggt
     3001 ggcctcgctc cagctgttca tccagcgctg cttcctgcag ctcgagctgt tcaacgtcga
     3061 cagcgacagc gtctggaagc agtgggactg gatgaagcga ttccggatgt gggaggccaa
     3121 ccggaagatc ttcctctatc cggagaactg gtacgatccg gcgcagcggc gcgacatgtc
     3181 gccgtacttc caggccctgg tcaatgacct ccagcagacc aacctgacca acgacgtcgc
     3241 cgaggacgcg ctccgcgggt acctgcacaa gctggtcgac gtcgcccacc tcgaggtcag
     3301 cggcctgtgg gaggagccgg tgcagggaag cgcccagtcg atcctgcacg tggtcgcgcg
     3361 cacgcggaag acgccgtacg tctactcgta tcgccggcgg gaaaccacgg gggggtggtc
     3421 cgcgtgggag ccgctcgatg atgggaccaa cgcggatcac atcatgccgg tgatctggaa
     3481 caagcggctg ttcatgctgt ggaccgagtt caccgagaag ccacagccgg tcgcggatgc
     3541 cgatcacgcg gtcccgagca gtgcagcggg cacgctcgtg ccgctaccca agaaatactg
     3601 ggagctcgcg ctgtcgtgga tcgagcgtcg caacaacatc tggctgccca agcggcagag
     3661 ccagcgcaag cagatcatca acggcgtgtt cagcggcagc accaaccagg tcttcaagtg
     3721 gcaggtcgcg ttccgggcca ccacgccgct gcccaccgga gcgcccaccg gaaccacgct
     3781 gtccaatctc gcgctgtcgc tctacgagcc gaccgggccc agctcgcagg cgctggccca
     3841 gtggatcctg accagccccc aggacgaacc gcagctgctg aacaccgacc tgaccacgct
     3901 cgccacggtg gcggtggaag acatgaagta cgtcgccgcg ctgccgcccg gcacggccca
     3961 gatcggcgcg cggccgaagt ttccgaacac ggccggcagc ccggtcgaca tgaattacgt
     4021 cttcaatgcc ttcggtggcg atgcacccaa cgtgacggga tcactggggc tccagcggcc
     4081 ggtcgggtcg atcccgctcg tcaccatcct gaacaagatc acgatggcgc gggtgatcag
     4141 cgtgcgctac ggcgacatca accagttctc ctcgccattc ttcatctcgg atccccagcg
     4201 cacgttcttc gggaccttca ccacctccag caactggaag ctgcagacgt tctaccaccc
     4261 gtacgccgac gccttcgtcc agcagctcaa ccagggcggg atccccgggc tgtatgaccg
     4321 ggcgctccag gtcaaccccg acgcggcgcg cggcctgacg gcggcgttcg acttcaacgc
     4381 gacgtacacc ccgaacaccg atacgaccat cctggcgacg ccctatccga cagagacgat
     4441 cgactacacg cagtcggggc cctacgcgat ctacaactgg gagctgttcc tccacacgcc
     4501 gctcttgatc gcgaagcggc tcgccgacaa ccagcggttc gacgaggcgc tgcgctggtt
     4561 ccactacatc ttcaacccca acacggtgaa cgtcccgggg tcgagctcgc cggggcccgt
     4621 gcctgcccgc tactggaacc cgaaggtgtt ccgcgacctc gtgagtgccg actacaccgc
     4681 ccagcagatc gagcaggtgc tcgcgctggt cgaccagggg gtcgccgacg cggtgcagcg
     4741 ggtctccgac tggcgcaata acccgttcga tccgaacctg gtcgcggcgg cgcggccggt
     4801 tgcctaccag aaggccgtcg tgatggccta catctcgacg ctcatcgcct ggggtgatca
     4861 gctgttccgc ggcgacaccg tcgagtcgat caacgaggcc acgcagctct acctgctggc
     4921 gagccagctg ctggggccgc ggccgcagag cctgcgcgcg atccagccca agcagaccag
     4981 caactatcag gatctcggca cgtcgatcga cgcgttcgcg aacgtgatgg tcaacatcga
     5041 gaacatcgtg tcgctggcac ctccgacgac caccggaagc ccgcccacgc cgacgatgct
     5101 ccacacgttc tacttctgca tcccgcccaa cgatcagatg ctgagctgct gggacacggt
     5161 cgccgaccgg ttgttcaaga tccgccacgg catgaacctc gatggcgtgg cccggccgct
     5221 ctcgctgttc gaggcgccga tcgatccggg gctgctggcg cgcgccgcgg ccgcgggcat
     5281 cgacatctcg gccgcgctcg cggatgccgc ggccagcgtc ggctgctacc ggttccagac
     5341 cctgtggcag gcggcccacg atctgtgcca ggacgtccgc ggcctgggca gcgcgatcct
     5401 gtccgcgacg gagcggaagg acgccgagga gatggcgcgc ctgaaggcga cgcaggaggt
     5461 cgcggtgacc accgcggtgc gtgaggtcaa ggccaagcag ctcgaggaag ccagggccag
     5521 caagaccgcg ctgctgagca cgcgcgcgat ggcggagatc cgcaaggcgt actacgagga
     5581 tcccgcgcgt cgcaacctca tgaacgacgc ggagacccag gcgcaggacc tcaacacgca
     5641 ggcccagcac aacgaggagg ccgcgcaaga tcacgatcgg aatgcctcca ggacggcatg
     5701 gatcccgtcg atcaccgtcg gagtcagcgg attcggtggt tcgcccacgc tccataccac
     5761 cttcggagtc gagaactacc gggcccacgt cgccgcgctg accgccgtgc tccgtgacaa
     5821 ggccggtatc aacagccgaa aggccggcca gtcgacgacc ttcgcgagct accagcgccg
     5881 caaggacgaa tgggacctcc agctcgcgct cgtgacccag gagctcgatc agctggacaa
     5941 gcagctgatc gccgccgacc ttcgcatcgc gatcgccgag aacgagctcg cggtccagga
     6001 ccgccagatc gcggacgcgc aggacaccgc ggcgctgctc cagtcgaagt tcaccagcca
     6061 ggagctctat acctggatgg tgtcgcagct gtcgtcgaca tacttccagg cctatcagct
     6121 cgcctacgat ctggccaagc gcgccagcaa ggcgtactcg ttcgagctcg gccagcccga
     6181 cccgggcttc atccagttcg gctactggga cagcctgcac cagggcctgg tcgcgggcga
     6241 caagctgctg ctcgatctgc gccggctgca ggccgcgcac ctgaacaaca acacgcgcga
     6301 gctcgagctg accaagcacg tgtcgctgct ccagctcgac ccggagcagc tggtcaggct
     6361 ccgcgagacc ggatcgtgcg acttcatcct gcccgaatcg ctgttcgacc tcgatcagcc
     6421 cgggcacttc taccgccgcc tcaagacggt gagcgtcacg ctgccgtgcg tgaccggtcc
     6481 gtaccacggc gtcaacgcga cgctgacgct ggtcggtcac tcgatccgga cctcgacgaa
     6541 cgcgagcgga tatggcacga cgacgaccga caacggcttg ccgtccgacc cggcgcgctt
     6601 cacgtacgtg gcctcggaca accggtcgat cgcgatcagc accggccgcg aggacgccgg
     6661 catgttcgag gtcaacctgc gcgacgagcg ctacctgccg ttcgaggccg ccggcgcgat
     6721 cagccgctgg cacctcgacc tgcagcagca gaacaaccgg ttcgatctcg cgacgctgag
     6781 cgacgtggtc ctccacgcgc gctacaccgc gcgcgacggc ggcggtgcgt tgcgccaggc
     6841 ggccagcgat gcggtgagcg cgaaccctcc gcccggcgcg cgggtgcagc tgctcagcgc
     6901 gcgcaccgag ttcccggacg cctacgcgcg cctgttcgca ccgaccggat ccggacagcg
     6961 cctcgacctc gcgctcgccg cgcagcactt cccgttcgtc cccgccagcc agcagatcac
     7021 catcacggga gtcctcgcga tcctgacgtt caccaacgac aagaactaca ccgactacgc
     7081 gaacctgact cccgggctgc ggctcaccgc gcgcctcggg ttcacccccg gcgacgggac
     7141 actgcccacg gcgtccaagc cgttcgcgcc tgaaaccacg cacctcagcg gtgtgccggc
     7201 caccgacgcg gtcgcgctgt ccggaccgat cggcccggtg acgatcgggt tcgtcgagag
     7261 cgagctcgtc aacgcgacca cgttgctcgt ccagcccgag acccagccgg acagcacggt
     7321 ccttcaccgc ctgatccgcg acaagatcga cgacctcttg atcgtgatca acttccaggt
     7381 ggggcccaag acctagtccg gcgttcgagg agcggccgct gcgcagcggg cggccgggtg
     7441 caagccggcg acggtcgagg accgccgcca tcggcggtcg tctggccgtc gtgttcttga
     7501 agctacgagg tgttgcaatg accgacaaca aggatcgata tggaagcggc gaccgcgcgg
     7561 cgcgccgggc cgacagcgga agcagcggct cgggctcgac gacgagcacc gccagggacg
     7621 gacggttctc cgctccagcg ctatctctac ccaagggtgg cggcgcgatc aggggcatcg
     7681 gcgagaagtt cgcggctcat cccgccaccg gcaccggcaa gctctcgatt cctctgccgc
     7741 tcagtccggg gcgcgcggga ttcacaccgg cgctgtccct cgactacgac tccggtgccg
     7801 gcaacggacc ttatggcttt ggctggaagg tcgattatcc ggcgatcacg cgccgcaccg
     7861 atcagggact gccgcaatac cgagatgatc gcgagtccga tgtattcctg atctcgggcg
     7921 ccgaggacct ggtgccggtg accaagggcg acggctcgcg cgacgagctg gtgcggaacg
     7981 gctatcgggt cacgcgctac cggccgcgca tcgaaggtct gttcgcacgc atcgagcgct
     8041 gggccagcct gaccgatcgc gcggacgtgt tctggcgctc gatcagtcgc gacaacgtga
     8101 cgacgacgta cggcaagacg gccgcgagcc ggatcgcgga tccggccgat tcgacgaaga
     8161 tcttcacgtg gctggtgtgc gaatcgttcg acgacaaggg caacgcgtcg acctacgagt
     8221 acatcgccga ggacagtcgc aacgtgaatc gcgggctggc cagcgaggcc catcgcagcg
     8281 acgcgagccg gggcacccag cgctacctca agcgcgtgaa gtacggcaac cggacgtctc
     8341 gcctcgtcca gcccgatctg tcgaccatgg agtggctgtt cgagctggtg atggactatg
     8401 gcgatcacag cggtgaagcg ccgagcgtcg cgccggacca ggactgggcg gtccgcccgg
     8461 atccatgttc gatggcccgc gccggcttcg aggtccggac gtatcgccgc tgccggcgca
     8521 tcctgatgtt tcatcgcttc gccgagctcg ggatctcccc gcgcctggtg cgctcgctgg
     8581 agctggacta cgacgacttc acctacccgc cggggttcga tgtccggacc gagctcgcgt
     8641 ggcaaggcag cacacgcacc gcgtcgctgc ttcgccgcgt gacatcgttc ggtcacgccg
     8701 acggcgggct ccgcacgtcg ctgccgccgc tcgagctgac gtactcgcgg ccacagatcg
     8761 tcgaggacgt ccagacgctg gccgacgaca gctacgccaa cctgccggtc ggagtcgacg
     8821 atcgccaggc cagctggctc gatctcgacg gcgagggcct gtcaggcgtg ctcagcgagt
     8881 acgagggggc atggtactac aagcccaacc tcggcaacgg ccggctcggg tcggtcaagc
     8941 ccgtcgcaca gcgaccgtcg atcgcgcgcc gcggccggac caagttcctc gatctcgatg
     9001 gcgatggcgc gctcgatctg gtcgagctcg accacccgcg cgccggcttc cacgagcgcg
     9061 gcgagcgcgg ccagtggtcg gcgttccggc cgttcatcag ccagcccgat gtgacgttcg
     9121 acgatccgaa cctccggttg atcgacctca ccggcgacgg ccgtgcggac gtgctgatca
     9181 cgcaggacga ccagctgtgc tggcatccct cgctgggcga gcagggcttc ggcccgcgcg
     9241 tcgccctgcg gatgcccgcc gacgaggcgc aggggccgcg cgtcgtgttc gaggacggca
     9301 ccgagacgat cttcctggcg gacatgtcgg gcgacggcct gtcggacatc gtacgcgtcc
     9361 ggcgatcgca gatctgctac tggccgaacc tcggctacgg acggttcgga cggcgcgtca
     9421 ccatggacag ctcgccggtc ctcgactcgc aggagcgctt cgacccgcga cgggtccggc
     9481 tggcggacat cgacggctcg ggggccgttg acctgatcta tctgggtgcc gacgcggtcc
     9541 ggatctactt caatcgcgcg ggcaatggct ggacggcgcc gttccagctc cgcgcgttcc
     9601 cggaattcga ccacacgtcg aacgtccagg tcgccgatgt tctgggcaac gggacggcgt
     9661 gcctggtgtg gtcgagcccc ctggcgcaca gtgcgccggt gccgcttcgc ttcgtcgacc
     9721 tgatgggcgg gcagaaaccg cacctgctcg tcgggatcga gaacaacttc ggcgcgcgca
     9781 cggtcatcaa gtacaccgcg tccacccagt tctacctcca ggaccagcag gccgggcggc
     9841 cgtggatcac gcgcttgccg tttccggttc acgtcgtcga gcgcgtcgag atctacgacg
     9901 acatcagccg gaatcgcttc gtcacccgct atgcctatca ccacggattc tacgatgggg
     9961 tcgagcgcga gttccgcggg tttggcatgg tcgagcagat cgacaccgag gagatcgccg
    10021 cgctggccgc cgaccagcag ctgccgccgg cgaccaacat cgatcagtcc tcgcaggtcc
    10081 cgccggtctt gacccgcacg tggttccaca ccggcgtgtt catcggcgcc gaccgcatct
    10141 cgcggttctt cgcgggcctg ctcgacggca ccgaccgggg cgagtactac cgcgagcccg
    10201 ggttgaccga cgcgcaggcc gcacagctcc tgctcgacga cacggtcctg cccccggggc
    10261 tcaccccgga cgaggcccgt gaagcctgcc gcgccctcaa gggcatgatg ctgcggcagg
    10321 agatctacgc gctcgacggg accgaccagc aaccgcaccc gtatgcggtc accgagcaga
    10381 acttcacgct gcgcctggtg cagcccaaag gcgccaatcg ccacccggtg ctgttctcgc
    10441 atccgcgcga gtcgatcgtc tatcactacg agcgcgtgcc cagcgatccg cgcgtggcgc
    10501 acacgttgac gctcgaggtc gacggcttcg ggaacaccct gaagacggcg acggttggct
    10561 atggccggcg gacggccgat ccggcgctgt ccgcagacga tcaagcccag caggccgcga
    10621 tccatatcac ctatttggac aatcaggtca ccaatgccat ccaggcgtcc gatgactacc
    10681 gagttccgct gcccagcgag gcgaagacct acgagctcac cggcctcgcg ctcgccgcgg
    10741 gcgccagccg gttcacgctc gatcagctga tcaacgcagg cacctccgcc gcggagatcg
    10801 cctacgagca gacaccgacc gccagcgtgg tggagaagcg gctgatcgag tgcctgcgga
    10861 cgctgtaccg gcggaacgac ctcaccgcgg cggcgccgtt cggaacactc gaatcgctgg
    10921 cgctaccgta cgagacgtac cggctcgcct tcacgcccgg cctcgtcacc accgtctacg
    10981 gcacccggat cacggacgcg acgctctcga ccgacggtgg ctatgtccac agcgagtccg
    11041 acgcgcggtg gtgggtcccg tcgggccgtg tgtcgttcag cgccagcgac gtcacccccg
    11101 cggccgagct gaccacggcc cagcagcact tcttcctggc gcggcgctat cgcgacccgt
    11161 tcggcgaggc gagcgcggtg acgttcgacg cctatgacct cttggtcgcc gagaccagcg
    11221 atgcgctggg caaccgcatc accgtcggcg agcgcgacag cgccggcaat ctcgtcagcg
    11281 ccggccatga ctatcgcgtg ctgcagccgc gcgtgatcac cgatccgaat gccaaccggt
    11341 cggccgtcag cttcgatgcc ctgggcatgg tcgtgggcat ggcggtcatg ggcaagcgcg
    11401 acgacagccc gcgccgcggc gacctcctgg acggcttcgt ccccgacctg accgacgatg
    11461 tcgtggccgc tcacctggcc gatccgctga gcaatccact tgcgatcctc gccagcgcca
    11521 cggtgcgcac ggtctacgac cggttcgcgt attaccgcac caagcagacc gcgcagcccg
    11581 cccccacggt cgtctacaca ctcacccggg agacccacga cgccgatctc tccccgggtc
    11641 aacaaacgaa gacccagcat cgcttcgcgt actcggatgg cttcgggcgt gagatccagc
    11701 acaaggccca ggccgagccg ggctcggtgc cggtgcgcga tggcaacggg cagatcatcg
    11761 tggtcaacgg cgtcccacag ctcacgacca cggccgtatc gccgcgctgg gtcggcagcg
    11821 gctggaccgt gttcaacaac aagggcaagc cggttcgcca gttcgagccg ttcttcaccg
    11881 accgttcgag cttcgaattc gacgtccgca tcggtgtcag tccgatcgtc tgttacgatc
    11941 cgatcggccg cgtggtcgcg accgtgcagg caaaccacgc ctgggagaag gtgagcgtcg
    12001 gggcgtggca gcaggtgacc tgggacgtca atgacaccgc gctgatcgca gatcccaggg
    12061 aggatctcga cgtcggcgcc atgctgggcc ggctcgacag caacgatctg ttgccgacct
    12121 ggtatgcgca gcgccagggc ggcgacctgg gctccgacga gcaggacgct gccaccaaga
    12181 ccgccgcgca tgcagcgact cccacgatcg cgaatttcaa ttcgctcggg agaacgttcc
    12241 tgaccatcgc gcacaaccgg ttcaagccca gcgatgcgcc gcccggcgat ccaccggtcg
    12301 aggagcatta cgtcagccgc gtcgtgctcg acatcgaggg caacgcacgc cggatgctcg
    12361 acgccgcggg ccggaccgtc gtgcagtacg actacgacat gctcggtcac cggctccacc
    12421 aggcgagcat ggaagccggc gagcactgga agctcgagga cgtgctcgga aagccgatcc
    12481 acagccacga cagccgcggc catcacaccc gtaccgagta cgatgcgctg cgacgcccgc
    12541 tgcgaacctt cgtgctgggg gcagacccgc agcgccccac gaccgagctc ctcgtcgtca
    12601 agaccgatta cggcgagggg caaccgagcg acgtccagct caacctgcgg acccgagcat
    12661 atcgccagta cgatggcgcc ggcatcgtca tgaacgatgc ctttgacttc aagggcaacc
    12721 cgctgcagac ccaccgccag ctggtgacgg actacaaggc ggttgcggac tggtccacga
    12781 cacccgcgct gcagcaggac agcttcgcga gcagcaccac ctacgacgcc ctcgatcggc
    12841 cgctcagcgt caccaccccg gacgcgagca tcgttcgtcc gagcttcaac gaggccaacc
    12901 tgatcgagcg cgtagatgtt cagctgcagg gtgcacagac caccaccgcg ttcgtggcca
    12961 atatcgacta cgatgccagg ggccagcgta cgcggctcgt actcggcaac aacgtcacga
    13021 ccgactacgc gtatgaccgg ttgacgttcc ggctgaaacg gctcgtgacc acccgctcga
    13081 ccgatcaggc gacgctgcag gatctcggct acacctacga cgcggtcggc aacgtcacga
    13141 ggatccacga tgccgcccag cagacgatct acttcaacaa ccaggtcgtc accccggata
    13201 gcagctacgt ctacgatgcc gtgtaccggt tgatccacac cgacgcgcgc gagcacatcg
    13261 gccagtcgtt gccgacccag accacgtgga acgaccagtt ctacatcaac ctgccgcacc
    13321 ccggcgacgg ccaggccatg cggcgctacg gcgaggacta tcgctacgac gcggtcggca
    13381 acctcctgca gctcgtccac caggcatccg gcgggagctg gacgcgcagc tacacgtatc
    13441 aagagaccag ccagctcgaa ccgggcgccg ccagcaaccg cctgagccgc accgcagtcg
    13501 gcagcgatcc tccagatctc tatacccacg acgtgcacgg caacatgacc gcgatgccac
    13561 atctcgcggc catgcagtgg gattaccagg atcggttgag cacgacgtcg cgacaggtgg
    13621 tgaacagcgg gacgccggag acgacctact atgtctatgc gtcgaccggc gagcgcgtcc
    13681 gcaaggtcac ggaacgccag aacggcacgc gcaagagcga acggatctat ctcggcggat
    13741 tcgagatcta tcgcgcctac gacgccagtg gcgccacggt gaccctggag cgcgacacgt
    13801 tgcacgtgat ggatgaccat cgtcgcatcg cgctcgtgga tacccgggtc gtcggaaccg
    13861 agtccggcgt gccgcagcag ctcgtgcgat accagctcga caaccagctc ggttccgcca
    13921 cgctggagct ggacgatcag gcgcagatca tctcctacga ggagtattac gcgtacggca
    13981 gcacgtccta ccaggcgggc cgcagcgtcg ccgaggtcag cctgaagcgt taccgctata
    14041 caggcatgga gcgcgacgag gagacggggt ttgcgtacca cggcgcgcga tactacgcac
    14101 cgtggctggg caggtggctg tctgcggacc catccggggt cgacaacgac ggtcccaacc
    14161 tgtacgccta cgtccacaat aaccccatca cctacgtcga tccatccgga ctcgagggcg
    14221 aggagcccca aaagggccag tttcaatggg ccaagaccaa ggaagagaac gacgctcgag
    14281 acgagcggct ccaggggctg ttcgagtacc tgaaggtgca cggggtgggc gccgggagcc
    14341 tcgagaagca cctcgagaag cacggggatc tcgagatcct caagcactac ggctacgaaa
    14401 aacccagtgt cctcgattca ccgaatgccg ataatcgacg ggagatgaac cagcgcgcgg
    14461 tgatgcgcgc gatatacaac tatcattacg agcacatcgg aaccccccaa accgaggcca
    14521 agccgctgac gcaagcgaac gtcgatgccc acaacgccgc ggtcgcggag ggccagcgga
    14581 tcgagtcatg gacgaacacg acactggccg gtgcacacac ctcggtcatc ggggcaggcg
    14641 tcgccgaggt cacgggccat ttcacggacg atccgaaaaa gatcgacgcg gggcggagga
    14701 caggctctct cgtgggcgat gtcgggctca ccttgtcccc ggtcattgca cagcggctcg
    14761 ccgctctgcg tgccctgagc gccgcttcgc aagctcccgt gaatggctcc ggcgggtcga
    14821 tcaagagcgt cggcggcgga acctcttcgc cgggctcccc gttgacactg gtcggcagtg
    14881 gcggcagcgg cagcggcttg caaggcagac tggtccttgt cacctcgtgg gcatcgcgcg
    14941 gcatcacgcc ggacctgaga ccggggcggt gggtgcagct gggaggaccg acctggctga
    15001 atttcttgaa gaccggtctg atcgggccaa aggcgtacct ctctggtaca ttcccatacc
    15061 tgcgaatcgc accatcgaac gttccattct ccaattcgat ttccggactg gtccctccgt
    15121 cttcgctcag ctggccaccc ggtgtcgaag cgtggaaggg gctcctcgga cagcggatat
    15181 tgaagccatg acctatatcg cattcattgt caccggcctc ttcctgatga agatcctgtg
    15241 gaatgtcacg gtcccgtaca ccctggccgt gagatcgttt cgcgccaagg acggcgaatc
    15301 ccgtggcatc tcgctgttgc cgtttgtcga gctcgcgctc ctgggagtcg ccgcggggct
    15361 gtccttgacc gagcgcggtt ctccctggtc gtggagcatc ggtacgaccg tcctggtggg
    15421 aggtgcgatc gccgtcgcgt cctacgtcca cctcatcatc gcgggggcga tcgcagggtg
    15481 ggtcgtcaag aagctccgcg cacgccgcgg gcggtgaccg ctctgtcccg ttcacatccc
    15541 cgtggcagac ggtgagaggg caatgatgat cgacgagacc gcggcacaga acgcgctggc
    15601 tggcctgttt cgggccggcg tcgggcactg gaacatcctc accggcgtgg cgcaggcgat
    15661 gtcggtggcg ggcaagcagc gcctcgacat cgtggacttc ttctggcggc tgatgaacgc
    15721 atcgcccgcg gtgctcgacg aggagcagct gaatctcgtc gacgacttca ccggtgcgct
    15781 gctcggcaac tgtccgctgg atcggatcat ccggctgcac ggcgatccgg aggaccccca
    15841 ggcgctcggc gagctcgtcg tcgcggacgc cgcgcgctgg aagccgcccg gctcgtagcg
    15901 gtcgtccgac cgcgtcaccg gctcaggcga cggcgaggtc ggcgatcggg ctgtcgccgg
    15961 cggcggcgag gccctggagg cagcggacca ggtgggcgcg gatcttgtgc ttcttggagt
    16021 acacggtctt gaggctgatc tgcatctcga ccgcgatctc gtcggcctcc atcccgcgct
    16081 ggtagtagag ctcgacgaac gtgcggtcct tgtcggtgaa gtcggagagc agcgagttga
    16141 ggtgctccca gcgctccttc tcgatcagct ggtcgagcgg ggtgcggtcg cactcctcgt
    16201 gcgggtcgag catgccgtcg accttgtcga gcagcgggcg gcggccggcg ctgcgcagga
    16261 agtcgtaggc ggcgttgacc gagatcatgc cgatccacga gccgagcttg gtgccgcgcg
    16321 ccgggttgta gatccggagc ttgtgcatgt cgtcgcgcac cagctgcatc atcacgtcgg
    16381 catagatctc atcgagatca gccgccgaca ggctcggcgc gtacttgagc gtgaccttgg
    16441 tgatgcagcg gtagatcagc ggccggtagc gccggatcag ctcgctccag gcgcgagact
    16501 cgttccggag gacgcaccgc agcagctcgc gatccgacca gtccgcaggg ttcttcgagg
    16561 cgcgtgtcgc gagaagagcc atgtgcattc ctccgggccg aacggaggag caacccggat
    16621 gccacgccta accgactgga tttatttggc gaagcggctc catgaacggt gcattcgacc
    16681 aagaattggt gaaatcgacc agcgctgcgg cgagatcggg aagaaccccc ggcgacgcgg
    16741 cgtactaccc actcccggtg catgcagatc ttgcctgcgt ctcgatcctg gcccaggccg
    16801 gccctggacc gggccacgtc cggcctggcc gggccgggtt tctcggatga ggtgcgatgt
    16861 gggtggcgcg cagcgtcaat cgcagcggtg gcgtcccggg gggccgggcc aggtcacgcc
    16921 ttgcgaaccg cggcgtccca gcgcgcgagg tgctcggcga cgcgggcgcg gtctgccgcc
    16981 ggcgtgaacc gcttctgctc gcgccaggtc ttctggacct gtgcctgatc cttccacacg
    17041 ccggtgccca ggccggcgag gaacgcggcg cccagggcgg tggtctcgac caggtcgggg
    17101 cgcgagatct cgacgccgag cacgtcgctc tggaactgca tcagcaggtc gttggccgag
    17161 gcgccgccgt cgaccttgag cacggtgagc ggccggccgg cgtcgcgctc catcgcgcgc
    17221 aggatgtcga cgttctgcag cgcgatgccc tcgagcgtcg cgcgcgcgat atggccgcgg
    17281 gtcgcgccgc gggtcaggcc ggtgatcagg ccgcgcgcct cgggccgcca gtgcggcgcg
    17341 ccgagcccgg cgaacgcggg gacgacgatc acgccgccgg agtcgggcac cgaggcggcc
    17401 agcgcctcga cgtcggcggc cgacgcgaac agctggaggc cgtcgcgcag ccactgcacc
    17461 gccgcgccgg cgatgaacgc cgagccctcc agcgcgtagc gcagctcgcc ggtcgcgagc
    17521 ttccaggcca ccgtggtgag caggccgctg tgcgacgcga tcggccggtc tccggtgttc
    17581 atcaggatga acgcgccggt gccgtaggtg cacttggcat cgcccggggt gaagcaggcc
    17641 tggccgaaca gcgccgactg ctgatcgccg gcgagcccgg cgatcggcac gccgtcgcgg
    17701 atcccgggca cgccgcgcgt caccccgagg gtgcccgacg actcgaccac gccgggcagg
    17761 accgcgcgcg gcgcgccgat cagctcgagc atctcgtcgg accacgccag ctggtggagg
    17821 tcgaacagca gggtgcgcga cgcgttgctg acatcggtgg cgtggattgc gccgccggtc
    17881 agccgccaca ccaggtagct gtcgatcgtg ccgaacgcga gctcgcccgc gctcgccgcg
    17941 gcggccgcgc cgggcacgtg gtggagcaac cacgcgagct tggtgcccga gaagtacggg
    18001 tcgagggtga ggccggtcag ctcgcggacg cgggggccct tgccggcggc ggtgagctcg
    18061 gcgcagcgcg ccgccgtgcg gcggtcctgc cagacgatgg cgcggtgcac ggcctggccg
    18121 gtggcgcggt cccacaccac ggtggtctcg cgctggttgg tgatgccgat ggccgccacc
    18181 gaggcggggt ccacgccctg gagggccgcc tggagggcgc tgcacaccga ggcccagata
    18241 tcctcggggt cgtgctcgac ccagcccggc tcggggtaga tctggcggaa ttcctggtag
    18301 ccgcggccg
//
LOCUS       DASVOR010000795         2859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_95893,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000795 DASVOR010000000
VERSION     DASVOR010000795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2859
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95893"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2027
                     /locus_tag="VF516_15780"
     CDS             <1..2027
                     /locus_tag="VF516_15780"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX8109194.1"
                     /translation="PGAWGGERSGSEPTLSDRVADYQLRAVVDPVKHTIEGSEKLRWR
                     NRSDRAVRTLYFHTYLNGFEGPGSTWLTEKARYGQFRSGVELKPGEAGYIEVRSIVQG
                     GAQGGQPAAMRYVQPDGGPPTDRSVLRVDLPSPVAPGGETSLDIAFHDQLPRVVARTG
                     YFGSYHLVAQWFPKIGVLELAGERGAQGPRWNCHEFHLHSEFYADWGSYDLEIVAPRG
                     FTVGATGARTGEPADTSAGAVHRYHQDDVHDAAFTVWDGFAPALRGSYDGQGSPHVDV
                     QVLYPPEYPSSGRVALQATIDALGYFSRTLGPYPYKQVTVVVPPYNATESGGMEYETF
                     FTTIGGGPGFDAAVRYVTVHEFGHGYFMGLLASNEFEEPFLDEGMNEWWDSRMMENED
                     LTFDFGLAGRLGFRLPAIHIWDFQWLGGSYDAGRPLADPIAGSSWQRMSAGTYGAVYP
                     RTALVMHDLEHQLGGDAIARGMKLYYARWHHRHPSAADLRDALADAAPEHRDAIQAWF
                     DQQVYASQLVDDEIDRVESTEILPEPGRIVKDGKTLERTRDEVDREIADRRAAFHAGS
                     DDVGPFPFRSVVRAQRHAAQVPQTVVVEFSDGSQERIAWPVGERWHRWVFERPVKVTQ
                     ARLDPAGALLLDVNKLDDGRTREPHRFPATRMAVEATTWVDVLLSLVSSL"
     gene            2024..>2859
                     /locus_tag="VF516_15785"
     CDS             2024..>2859
                     /locus_tag="VF516_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109195.1"
                     /translation="MKTRTIIGRAMRRALQWRLLVLSPVVLWAAAVATLLPLLHFLGG
                     LFDHSTRWRELAASLDSAALAGLAKAMMTPAAAGLPAGIGTSLVLTALLAPFLAGAAL
                     VATEDDVRPRFPALLSGAAGYYGRMFRLQIAALLPLAVAMVIASLAIAWSSGVSDRAM
                     SEAATHISFRIAVGVAALAVFLAQLVADAGRARLAVEPLRRSALFALGAGIKLVVKQP
                     LRALAVGLPATLISLAVAAVLLVVRQQIAQSSVAALVLAFVFAQLAVASIAWGHAARL
                     CGL"
BASE COUNT          361 a         1025 c         1055 g          418 t
ORIGIN      
        1 cgcccggtgc gtggggcggc gagcgcagcg gcagcgagcc gacgctgtcg gatcgcgtcg
       61 ctgactacca gctgcgcgcg gtcgtcgatc cggtcaagca cacgatcgag ggcagcgaga
      121 agctgcgctg gcgcaaccgc agcgatcgcg cggtgcggac gctgtacttc cacacctacc
      181 tcaacggctt cgagggaccg gggtcgacct ggctgaccga gaaggcgcgc tacgggcagt
      241 tccggtccgg cgtcgagctc aagccgggcg aggcgggcta catcgaggtg cgctcgatcg
      301 tgcagggcgg cgcgcagggc gggcagcccg cggcgatgcg ctacgtccag cccgacggcg
      361 ggccgccgac cgatcgcagc gtgctgcgcg tcgacctgcc cagcccggtc gcccccggcg
      421 gcgagacctc gctcgacatc gcgttccacg atcagctgcc gcgcgtggtc gcccgcaccg
      481 ggtacttcgg cagctatcac ctggtcgccc agtggttccc caagatcggc gtgctcgagc
      541 tcgccggtga gcgcggcgcg cagggcccgc ggtggaactg tcacgagttc cacctgcact
      601 ccgagttcta cgccgactgg gggagctacg acctggagat cgtggcgccc agggggttca
      661 cggtcggcgc gaccggcgcg cggaccggcg agcccgccga cacctccgcg ggcgccgtgc
      721 atcgctacca ccaggacgac gtgcacgacg ccgcgttcac ggtgtgggac ggcttcgcgc
      781 ccgcgctgcg cggcagctac gacgggcagg gcagcccgca cgtcgacgtc caggtgctct
      841 acccgccgga gtacccgagc tcggggcgcg tcgcgctgca ggcgacgatc gatgcgctcg
      901 gctacttctc gcgcacgctc ggcccgtatc cctacaagca ggtgaccgtg gtcgtgccgc
      961 cgtacaacgc gaccgagtcc ggcggtatgg agtacgagac gttcttcacc acgatcggcg
     1021 gcggccccgg gttcgacgcc gcggtccgct acgtcaccgt gcacgagttc ggccacggct
     1081 acttcatggg cctcctggcg tcgaacgagt tcgaggagcc gttcctcgac gagggcatga
     1141 acgagtggtg ggactcacgg atgatggaga acgaggatct gacgttcgac ttcgggctcg
     1201 ccggccggct cggcttccgg ctgccggcga tccacatctg ggacttccag tggctgggcg
     1261 gcagctacga cgccggccga ccgctcgccg atccgatcgc cgggagctcg tggcagcgga
     1321 tgagcgcggg gacctacggc gcggtctatc cccgcaccgc gctggtgatg cacgacctcg
     1381 agcaccagct cggcggcgac gcgatcgcgc gcgggatgaa gctgtactac gcgcgctggc
     1441 accatcgcca cccgagcgcg gcggacctgc gcgacgcgct cgccgacgcg gcgccggagc
     1501 accgcgatgc gatccaggcc tggttcgacc agcaggtcta cgcgtcccag ctcgtcgacg
     1561 acgagatcga tcgggtcgag tccacggaga tcctgcccga gcccgggcgg atcgtgaagg
     1621 acggcaagac cctcgagcgg acgcgggacg aggtcgaccg cgagatcgcc gaccggcggg
     1681 cggcgttcca cgccggcagc gacgacgtgg ggccgttccc gttccgcagc gtggtccgcg
     1741 cccagcgcca cgccgcgcag gtaccgcaga ccgtcgtcgt cgagttctcc gacggcagcc
     1801 aggagcggat cgcgtggccg gtcggcgagc gctggcatcg ctgggtgttc gagcggccgg
     1861 tcaaggtgac ccaggcgcgg ctggatcccg ccggcgccct cctgctcgac gtcaacaaac
     1921 tcgatgacgg ccggacccgg gagccgcatc gcttccccgc cacgcggatg gcggtggagg
     1981 ccacgacctg ggtcgacgtg ctgctctcgc tggtgagctc gctatgaaga cacggacgat
     2041 catcggccgc gcgatgcggc gcgcgctgca gtggcggctc ctggtgctgt cgccggtcgt
     2101 gctgtgggcg gcggccgtcg cgacgctgct cccgctgctc cacttcctcg gcgggctgtt
     2161 cgatcactcg acccggtggc gcgagctcgc cgcgtcgctc gactcggccg cgctcgccgg
     2221 gctcgccaag gcgatgatga cgcctgccgc ggcgggcctt ccggccggca tcgggaccag
     2281 cctggtgctg accgcgctgc tggccccgtt cctggccggc gccgcgctgg tcgcgacgga
     2341 ggacgacgtg cggccgaggt tccccgcgct gctgtccggc gcggccggct actacgggcg
     2401 gatgtttcgc ctgcagatcg ccgcgctgct gccgctcgcg gtcgcgatgg tcatcgcgag
     2461 cctcgcgatc gcctggtcgt ccggggtgtc cgaccgcgcg atgtccgagg ccgcgacgca
     2521 catcagcttc cggatcgcgg tgggggtcgc ggcgctcgcg gtgttcctcg cgcagctcgt
     2581 cgccgacgcc gggcgcgccc ggctcgcggt ggagccgctc cggcggtccg cgctgttcgc
     2641 gctcggggcc ggcatcaagc tcgtcgtgaa gcagccgctc cgggcgctgg cggtcgggct
     2701 gccggcgacc ctgatctcgc tcgcggtcgc cgcggtgctg ctcgtggtcc ggcagcagat
     2761 cgcgcagtcg agcgtcgccg ccctggtgct cgcgttcgtg ttcgcccagc tcgccgtggc
     2821 gtcgatcgcg tggggccacg cggcccggct gtgcgggct
//
LOCUS       DASVOR010000796         5168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_358474,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000796 DASVOR010000000
VERSION     DASVOR010000796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358474"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1545)
                     /locus_tag="VF516_15790"
     CDS             complement(<1..1545)
                     /locus_tag="VF516_15790"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109196.1"
                     /translation="MRNHWICAPILGLAAALIGCADHPYDPGAPAVDPTAPSVHILAP
                     ARGTFAGDVQTLTVTGTATDDTAVAAVQVNGVAAALAGDGSWTATIPVSPGTQLIHAE
                     ARDAQGNVGKESRAVVVGPLQPIAAPVPRAIAAALSAQTFGAISRGITGFLQTGDLAA
                     AVAPLNPVIDVGGGPDCNYVQAAITQLSVGSASSVTLVPQPGGLALDVELDRVTLGMH
                     LAYSVICLDGGSDVTITASHVKVTGLLGVAVQAGAFDIALTGQNVQLTGVNVDLSGLP
                     GEVIGWLDLDHALASVVGWAAQQLVVPALDSALGSLNDTRTIDVLGTPVDIHVAPAQI
                     AFDASGAVIELDTTLRAHGDAGAPGYVFLPGQEPAMSTARGFALAVADDAANQLLTSL
                     WAARGLDHSFDLTTGSYGDIGKLYDRVELSAAVPPFVAASGGALRLTIGDLIASFKNG
                     DALATQIAVSAEVDVKVVADATGRPRLDVGTPTTYVDVLDEHVDGANVLSNAQFEVIA
                     SFALARV"
     gene            complement(1494..2000)
                     /locus_tag="VF516_15795"
     CDS             complement(1494..2000)
                     /locus_tag="VF516_15795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109197.1"
                     /translation="MLIGYAVGTGEPTERIIARAASQPKPHALDALRASLTAFALAPL
                     SFSVDGRPLSPTSVHAKIGLDSGARPMVVVLATFALPVGGELAIRSKDPRTTRISWQD
                     RASGRIALDRAPAQGHWYTAVASFLLPLVSPAASRLSSPPASPLVTGSSPCATTGSVP
                     PYLDSPPR"
     gene            complement(2141..>5168)
                     /locus_tag="VF516_15800"
     CDS             complement(2141..>5168)
                     /locus_tag="VF516_15800"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX8109198.1"
                     /translation="AIGGPHAIGRVGDFLLENDQIRLVIADTGVDPADPGKTTYGRVN
                     TTFGGTLVDADLRRPGGDGGHGNDQLAELLPGFAFMIINPTRVTVTRDGADGQPAEVT
                     VVGTPADLLQEVGLLDTGLLGTSSLQLSQTYRLPPGKRYVEIDTTIKNVSGGARPFPF
                     LDPTQLRDLGFDIPGIDQIQLSVPLGQLPLLGGEQDLFAPGAGFNIRFTIDHSYDQSA
                     GFPAFPGLVIDYLASRGKGVSYGLTMPRSPDNYVHAYAAGYPDQTLTPYSMLLPFTYA
                     GVAGVYMYRPPAQLGPGEQFTYTAYFVIGKGDVASVLDTIYELHGEPTGTFGGRVIDQ
                     QTSQPVAGANLIVLDAAGRVVDQIATDRNGSFLAHLAPGSYRYLAVTDDRLATEPAGV
                     TIAAGVQIGVGPIEMAPPATLVVSAIDELGRHAPAKIQLLGHVAPADRGKDPRTFLYS
                     LERGERQRPTAFDGTDRYVENAWWTVDGRVSAAVRPGTYDLIVTRGPEYEITQKTVVL
                     RPGAFTAAQVALVRAYQTDGWIAGDFHIHAQPSTDSGLPIADRVTSCAAEGLEAAIST
                     DHNYITDYAPVIAQAGLDSWLLGIPGMELTTFEMGHFNGYPLRVDPGSTRGGEFRWAG
                     TPPQGLFDQLRALAIDSGTGTGSGTGSSTRRGIVQVNHPRQAVLGYFAQFFVDQATAE
                     PYTPSGILGIFAPYGDEFRPENYSTDFDAIEILTGSRLEDVHTFRAPDPLPPGPFPDP
                     QPVPGQVVVGPDGRPSFPGVVETWFTLLDRGHRATAMGVSDSHHLLGDEPGYARTLIY
                     VGAGKDVPGGTTRDDVIDAVRGHRAITTNAPFVELTIGGARIGDTITAPGGSVDVDLH
                     VRSPSWAKVDHLVVYANSQVVADQAIPAAQGTDYQTRVHLRLAVDSWVVAEATGSGNL
                     FPVVSAVEFPPLDATVLIKALSHGLNLGALPITSNLRPSSTHIVTPYALTNPIWIDVD
                     GNGWTPPRPPLPRRTAPAGPPPDVRTRFDALPEISR"
BASE COUNT          712 a         1699 c         2040 g          717 t
ORIGIN      
        1 cacgcgcgcc agcgcgaacg aggcgatgac ctcgaactgg gcgttggaca gcacgttggc
       61 gccgtcgacg tgctcgtcga gcacgtcgac gtaggtggtc ggcgtgccga cgtcgagccg
      121 cggccggccg gtggcgtcgg cgacgacctt gacgtcgacc tcggcgctga ccgcgatctg
      181 ggtggcgagc gcgtcgccgt tcttgaagct ggcgatcagg tcgccgatcg tgagccggag
      241 cgcgccgccg gacgcggcga cgaacggcgg caccgcggcc gacagctcga cgcgatcgta
      301 gagcttgccg atgtcgccgt agctgccggt ggtgaggtcg aagctgtggt cgaggcccct
      361 ggcggcccac aggctggtga gcagctggtt ggcggcgtcg tcggcgaccg cgagggcgaa
      421 gccgcgagcg gtcgacatcg ccggctcctg gccgggtagg aacacgtagc ccggcgcgcc
      481 ggcgtcgccg tgggcgcgca gcgtcgtgtc gagctcgatc acggcgccgc tggcgtcgaa
      541 cgcgatctgc gccggggcga cgtggatgtc gaccggcgtg ccgagcacgt cgatggtccg
      601 ggtgtcgttg agcgagccga gcgcgctgtc gagcgccggc acgaccagct gctgcgcggc
      661 ccagccgacg accgacgcga gcgcgtggtc gaggtcgagc cagccgatga cctcgcccgg
      721 caggccgctg agatcgacgt tgacgccggt cagctggacg ttctggccgg tcagcgcgat
      781 gtcgaatgcg ccggcctgga cggcgacgcc gagcaggccg gtgaccttga cgtggctcgc
      841 ggtgatcgtg acgtcgctcc cgccgtccag gcagatcacc gagtacgcca ggtgcatgcc
      901 gagcgtgacg cgatcgagct cgacgtcgag cgcgaggccg cccggctggg gcaccagggt
      961 gacgctgctc gcgctgccga ccgacagctg ggtgatcgcg gcctggacgt agttgcagtc
     1021 cgggccgccg ccgacgtcga tcaccgggtt gagcggcgcg accgcggccg ccaggtcgcc
     1081 ggtctggagg aagccggtga tgccgcggct gatcgcgccg aaggtctgcg ccgacagcgc
     1141 ggcggcgatc gcccggggga ccggcgcggc gatcggctgg agcgggccga cgacgaccgc
     1201 gcgcgactcc ttgccgacgt tgccctgcgc gtcgcgcgcc tccgcgtgga tcagctgggt
     1261 gcccggcgag accgggatgg tcgcggtcca cgagccatcg ccggcgagcg ccgcggcgac
     1321 gccgttgacc tgcaccgcgg cgacggcggt gtcgtcggtc gcggtcccgg tgacggtcag
     1381 ggtctggacg tcgcccgcga acgtgccgcg cgcgggtgcg aggatgtgca cgctgggtgc
     1441 ggtcggatcg accgccgggg cgccggggtc gtacgggtgg tcggcgcagc cgatcagcgc
     1501 ggcggcgagt ccaagtatgg gggcacagat ccagtggttg cgcatggcga gcttcccgtt
     1561 acgagcggcg acgccggagg cgacgaaaga cgcgaggccg caggcgacac gagaggcagc
     1621 aagaaggagg ccacggccgt gtaccagtgg ccctgcgccg gcgcgcggtc gagggcgatc
     1681 cggccgctcg cgcgatcctg ccaggatatc cgcgtggtgc gtgggtcctt cgagcggatc
     1741 gcaagctcgc cacccacggg cagcgcaaag gtcgccagca cgaccaccat cggccgcgcg
     1801 ccgctatcga ggccgatctt ggcgtggacc gaggtgggcg acagcggccg gccatcgacg
     1861 gagaacgaca gcggcgcgag ggcgaatgcg gtgagcgatg cacgcagcgc gtcgagcgcg
     1921 tgtggtttcg gctggctggc cgcgcgggcg atgatgcgct cggtcggctc gccggtgccc
     1981 accgcgtagc cgatcagcac cgccagctca caccgctcga cctggaccac gaccgtgtgc
     2041 accggcggga atacatgcgc ccaggctggt ggtccagcca gcgagatggc cagtacagta
     2101 gccaccccga cccgcgggag ggctcgctcg acgcggcccg tcatcgcgag atctccggga
     2161 gcgcgtcgaa ccgggtacgg acgtcgggcg gcggtccagc gggcgctgtg cggcggggca
     2221 acggcggccg gggcggggtc cagccgttgc cgtcgacgtc gatccagatc gggttggtga
     2281 gcgcgtacgg ggtgacgatg tgggtcgagc tcgggcgcag gttggaggtg atgggcagcg
     2341 caccgaggtt gagcccgtgc gacagcgcct tgatcagcac ggtggcgtcg agcggcggga
     2401 actcgaccgc cgacaccacc gggaacaggt tgcccgatcc ggtggcctcg gcgacgaccc
     2461 agctgtcgac cgccaggcga agatggacgc gggtctggta gtcggtgccc tgggccgcgg
     2521 ggatcgcctg atcggcgacg acctggctgt tggcgtacac gaccaggtgg tcgaccttgg
     2581 cccacgacgg cgagcgcacg tgtaggtcga cgtcgacgct gccgccgggc gcggtgatcg
     2641 tgtcgccgat ccgggcgccg ccgatcgtca gctcgacgaa cggggcgttg gtggtgatcg
     2701 cgcggtggcc gcggaccgcg tcgatcacgt cgtcgcgggt ggtgccgccg gggacgtcct
     2761 tgccggcgcc gacgtagatc agcgtgcgcg cgtagccggg ctcgtcgccg agcaggtggt
     2821 ggctgtcgga gacgcccatg gcggtggccc tgtggccgcg atcgagcagc gtgaaccagg
     2881 tctcgaccac gccggggaac gacgggcggc cgtcgggccc gacgacgacc tggccgggca
     2941 ccggctgggg gtccgggaac ggcccgggcg gcagcgggtc cggcgcgcgg aacgtgtgga
     3001 cgtcctccag ccggctgccg gtcaggatct cgatcgcgtc gaagtcggtc gagtagttct
     3061 cggggcggaa ctcgtcgccg tagggcgcga agatcccgag gatgccgctc ggcgtgtagg
     3121 gctcggccgt cgcctggtcg acgaagaact gcgcgaagta gccgagcacg gcctggcgcg
     3181 ggtggttgac ctggacgatg ccgcgccggg tgctggagcc ggtgccagag ccggtaccgg
     3241 tgccggaatc gatggcgagc gcgcgcagct ggtcgaacag cccctgcggc ggcgtgccgg
     3301 cccagcggaa ctcgccgccg cgcgtcgatc cgggatcgac ccggagcggg tagccgttga
     3361 agtggcccat ctcgaacgtg gtgagctcca tgccggggat cccgagcaac cagctgtcga
     3421 gccccgcctg ggcgatcacc ggcgcgtagt cggtgatgta gttgtgatcg gtcgagatcg
     3481 cggcctcgag gccctcggcg gcgcagctcg tgacccggtc ggcgatcggc aggccgctgt
     3541 cggtcgacgg ctgcgcgtgg atgtggaagt cccccgcgat ccagccgtcg gtctggtagg
     3601 cgcggaccag cgcgacctgc gcggcggtga acgcgccggg gcgcagcacg acggtcttct
     3661 gcgtgatctc gtactcgggg ccgcgcgtga cgatcaggtc gtaggtcccc gggcgcacgg
     3721 cggcggacac ccggccgtcg accgtccacc acgcgttctc gacgtagcga tcggtgccgt
     3781 cgaacgcggt cgggcgctgg cgctcgccgc gctccagcga gtacaggaac gtccgcggat
     3841 ccttgccgcg gtcggccggg gcgacgtggc cgagcagctg gatcttggcc ggcgcgtggc
     3901 ggccgagctc gtcgatcgcc gacaccacga gggtcgccgg cggcgccatc tcgatcggcc
     3961 cgacgccgat ctggacgccc gcggcgatcg tgacgccggc cggctcggtc gcgagccggt
     4021 cgtcggtgac cgcgaggtag cgatagctgc cgggcgcgag gtgggccagg aagctaccgt
     4081 tgcgatcggt ggcgatctga tcgaccacgc ggccggcggc gtcgagcacg atgaggttgg
     4141 ccccggcgac cggctggctg gtctgctgat cgatcacgcg gccgccgaac gtgccggtcg
     4201 gctcgccgtg cagctcgtag atcgtgtcga gcaccgaggc gacgtcgccc ttgccgatca
     4261 cgaagtacgc ggtgtacgtg aactgctcgc cgggcccgag ctgggccggc ggccggtaca
     4321 tgtagacccc ggcgaccccg gcgtaggtga acggcaacag catcgagtac ggcgtgaggg
     4381 tctggtccgg gtagccagcg gcgtacgcat ggacgtagtt gtccggcgat cgcggcatgg
     4441 tcaggccgta cgacacgccc ttgccgcggc tcgccaggta gtcgatcacc agacccggga
     4501 acgccgggaa cccggccgac tgatcgtagc tgtggtcgat cgtgaaccgg atgttgaagc
     4561 cggcgccggg ggcgaacagg tcctgctcgc cgccgagcag cggcagctgg ccgagcggca
     4621 cgctgagctg gatctgatcg atccccggga tgtcgaagcc gaggtcgcgg agctgggtcg
     4681 gatcgaggaa cgggaacggc cgggcaccgc cgctgacgtt cttgatcgtg gtgtcgatct
     4741 ccacgtagcg cttgccgggc ggcagccggt aggtctggct gagctggagg ctcgacgtgc
     4801 cgagcaggcc ggtgtcgagc aggccgacct cctgcagcag gtccgccggc gtgccgacca
     4861 cggtgacctc ggcgggctgg ccgtcggcgc cgtcgcgcgt gacggtgacg cgggtcgggt
     4921 tgatgatcat gaacgcgaac ccgggcaaga gctcggcgag ctgatcgttg ccgtggccgc
     4981 cgtcgccccc cggccgccgc aggtcggcgt cgaccagcgt gccgccgaac gtggtgttga
     5041 cccggccgta ggtcgtcttg cccggatccg ccggatcgac cccggtgtcg gcgatcacca
     5101 gccggatctg gtcgttctcc agcaggaagt cgccgacccg gccgatcgcg tgcgggccgc
     5161 cgatcgcc
//
LOCUS       DASVOR010000797         2182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_241457,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000797 DASVOR010000000
VERSION     DASVOR010000797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241457"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="secD"
                     /locus_tag="VF516_15805"
     CDS             <1..1176
                     /gene="secD"
                     /locus_tag="VF516_15805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEX8109199.1"
                     /translation="PPTTPPAGVDPKQYAQALAQFDKLTCAPNSGASASLDKPADVIA
                     SCDQNGGSKYLLAPAIIEGTEIAGASAVAPTSTSPQWTVSLDFKSSGQATWARYTSQH
                     NVTVTPNDPANNVAFVLDGKTISAPAIQGTINGQTQITGSFTQSQAEDLANVLKYGAL
                     PLTFQQQEALTVSPTLGSEQLKAGLLAGGIGVALVFIYSLLYYRALGLVMIASLILSG
                     LLVYAVLVILGRQIGFTLTLAGIAGFIVAIGITADSFVVFFERLKDEIRDGRSPRSAV
                     PRAWVRARRTILSADAVSFLAAAILYYLAAGTVKGFAFTLGMSTILDLVIVFLFTHPV
                     VTLFARNKAFMSPRVSGLGSVQRVAAQRAAAERAAAANQPPARSAAGRRGRVPAKES"
     gene            1189..>2182
                     /gene="secF"
                     /locus_tag="VF516_15810"
     CDS             1189..>2182
                     /gene="secF"
                     /locus_tag="VF516_15810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX8109200.1"
                     /translation="MFRRLDAGEPVFDFIGKRKRWYWVSAVLLLLSITSFIVRGFNFG
                     IEFDGGTQFQFRATTAQAADVQSVAEQAGADVQNTPQIVGAGGNRSILVKAGELSAPE
                     QTKVKNALQSRFGEQVTVQAVSSSWGSDITKAAIRGLVFFLIAVCIFIAIRFEWKMAV
                     GAIAALLHDLLLTAGIYSIVGFEVSPSTIVGLLTILGFSLYDTVVVFDKVDENARGIL
                     GGSRITYSGAANLAVNQTLMRSINTSLIALLPVAGLLFVGAGLLGVGTIKDLALVLFV
                     GLAAGAYSSIFLATPIVCELKEREPKYKALAKRVAQRRAAEARGEAPNGARRQKA"
BASE COUNT          321 a          792 c          717 g          352 t
ORIGIN      
        1 ccgcccacga ccccgccggc cggcgtcgat ccgaagcagt acgcgcaggc cctcgcgcag
       61 ttcgacaagc tgacgtgcgc gcccaactcc ggcgccagcg ccagcctgga caagcccgct
      121 gacgtcatcg cctcctgcga ccagaacggc ggcagcaaat acctgctggc gcccgcgatc
      181 atcgagggca ccgagatcgc cggcgcctcc gcggtcgcgc cgacctcgac cagcccgcag
      241 tggacggtct cgctcgactt caagagcagc ggccaggcga cctgggcccg ctacacctcc
      301 cagcacaacg tcaccgtcac gcccaacgac ccggccaaca acgtcgcgtt cgtgctcgac
      361 ggcaagacga tctcggcccc ggccatccag gggacgatca acggccagac ccagatcacc
      421 ggcagcttca cccagtcgca ggcagaggac ctggccaacg tgctcaagta cggcgcgctc
      481 ccgctgacct tccagcagca ggaggcgctg accgtctcgc cgacgctcgg ctccgagcag
      541 ctcaaggccg gcctgctggc cggcgggatc ggcgtcgcgc tggtgttcat ctacagcctg
      601 ctctactacc gcgcgctcgg cctggtgatg atcgccagcc tgatcctgtc cggcctcctg
      661 gtctacgccg ttctggtcat cctcggccgg cagatcggct tcaccctgac cctggccggc
      721 atcgccgggt tcatcgtcgc gatcggtatc acagccgact cgttcgtcgt gttcttcgag
      781 cgattgaagg acgagatacg cgacggcaga tcgccgcggt ccgcggtgcc gcgcgcctgg
      841 gtgcgggccc ggcggacgat cctgtccgcg gacgcggtct cgttcctcgc cgcggcgatc
      901 ctctactacc tggccgccgg cacggtgaag ggcttcgcgt tcaccctggg catgtcgacg
      961 atcctcgacc tggtgatcgt cttcctgttc acccacccgg tcgtcacgct gttcgcccgc
     1021 aacaaggcgt tcatgtcgcc ccgggtctcc ggcctcggat cggtgcagcg ggtggcggcc
     1081 cagcgggccg ccgccgagcg tgccgcggcc gcgaaccaac cgcctgcccg ttccgccgcc
     1141 ggccgccgcg gccgcgtccc ggcgaaggag tcctgagcca tgatcagcat gttccgccgg
     1201 ctggacgcgg gcgagccggt cttcgacttc atcggcaagc gcaagcggtg gtattgggtg
     1261 tccgcggtcc tgctgctgct gtcgatcacc agcttcatcg tccgcgggtt caacttcggc
     1321 atcgagttcg acggcggcac ccagttccag ttcagggcca cgaccgcgca ggcggccgac
     1381 gtgcagtcgg tcgccgagca ggccggcgcg gacgtgcaga acacgccgca gatcgtcggc
     1441 gccggcggca accggtcgat cctcgtcaag gccggcgagc tgtccgcgcc ggagcagacc
     1501 aaggtcaaga acgcgctgca gtcccggttc ggtgagcagg tcaccgtgca ggcggtgagc
     1561 tcgtcctggg gcagcgacat caccaaggcg gcgatccgcg gcctggtgtt cttcctgatc
     1621 gcggtgtgca tcttcatcgc gatcaggttc gagtggaaga tggcggtcgg cgcgatcgcg
     1681 gcgttgctcc acgacctgct gctcaccgcc ggcatctact cgatcgtcgg cttcgaggtc
     1741 agcccgtcga cgatcgtcgg cctgctgacg atcctcggtt tctccctgta cgacacggtc
     1801 gtcgtgttcg acaaggtcga cgagaacgcg cgcggcatcc tcggcggctc ccggatcact
     1861 tactcggggg cggcgaacct ggccgtcaac cagacgctga tgcgctcgat caacacctcg
     1921 ctgatcgcgc tgctgccggt cgccgggctg ctgttcgtcg gcgccgggct gctcggcgtc
     1981 ggcacgatca aggacctggc gctggtgctg ttcgtcggcc tcgccgccgg tgcatactcc
     2041 tcgatcttcc tggccactcc gatcgtctgt gagctcaagg agcgggagcc gaaatacaag
     2101 gcgctggcca agcgggtcgc gcagcggcgg gccgccgagg ccaggggcga ggcgccgaac
     2161 ggcgcccgcc ggcagaaggc ga
//
LOCUS       DASVOR010000798         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387470,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000798 DASVOR010000000
VERSION     DASVOR010000798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387470"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF516_15815"
     CDS             complement(<1..204)
                     /locus_tag="VF516_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109201.1"
                     /translation="MTWAHDVAPFTATSGIPALAAGQEIPCGAGCDAGRAAVAAEPGG
                     GVSACQRNLWRGAGLAGDDGSVGH"
     gene            337..1557
                     /locus_tag="VF516_15820"
     CDS             337..1557
                     /locus_tag="VF516_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109202.1"
                     /translation="MGLDLLRDRGTPIERQRFHWKELVQEPISKLDADALTRVRVILM
                     NGIESEANRFGHAAARLFPALQRPLAVIRRIEQHQQTLVNWLLPADQTPLETTIGYEQ
                     VAIEVTASVASAEPDPYMAAIFRFGLLEDFDHMYRFAALMDRTEGKDASTLLQGYTDI
                     LPGRPTTIEHRHPLDDLRSPYDRRHADLLSKLHALTIMAGENQTHDYYMTIGPMFADP
                     TARLLYAEIASIEEQHVTQYESLIDPRESALEKWLLHEACEAYNYWSCLEFEDDRRIR
                     AIWERMLDYELGHVQAVARLFEDHEGRDAEEVLERQIREPVQYASHRDLVRDVLRAEV
                     NLGADGSHLIDRTRDHEPERTRRYRAELNAEIQPSNVVAAGWTWQPGGELAARDTGKR
                     SDSNGVPPEQGGKS"
     gene            1554..2270
                     /locus_tag="VF516_15825"
     CDS             1554..2270
                     /locus_tag="VF516_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8109203.1"
                     /translation="MKRQVESSTAPGKNHTGLAPHAKERDDMVRGAQEFVPTSSGGAD
                     EIAQLRIAYARAGGPHGTMSAASVDAARLPVLDKLGARLVFERTGTRLYDALISKLDA
                     YGPFDGGPTRQQLVEIRDQEHAHALLAQDLIRALGGDPTMVTPCGNLQATASRGILDV
                     LVDPRTSLIDGLEAILIAELADQDSWAALARSAEALGDAGTTAKITQAQQTELQHLKK
                     VRGWLEAADRLRAKAATEVH"
     gene            2285..3124
                     /locus_tag="VF516_15830"
     CDS             2285..3124
                     /locus_tag="VF516_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109204.1"
                     /translation="MKELIDMPREDREYRNYRDDDDDRRRESHPFRGNQYTSGSSRDD
                     DRSYRSRGRDDDDERGGYRGSSRMPERDDYGRFVSDDDRRGGYRSRGRDRDDDDRDMR
                     SSGRGRGGWFGDPEGHSEASRRGWAERDDDRGGGRRMSEDRGGYRGSRMPERDEQGRF
                     TSDDDRRGRSPYEDRDDDRGGYRSRGRDRDDDDRDMRRSSGRGGWFGDPEGHSEASRR
                     GWERGDHGQSGWFGDSRGHAEASRRGWERGDHAQSGWFGDPEGHSEASRRGWEHGHNG
                     RGR"
     gene            complement(3154..>3649)
                     /locus_tag="VF516_15835"
     CDS             complement(3154..>3649)
                     /locus_tag="VF516_15835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109205.1"
                     /translation="SNGKPVSLLRIPKSAPALPPPSGRDTRFRSDGLSVTLAASMPIS
                     GYREGLAGGIVIAAPVDLAAVRRALDEHTAGASLIGLGSELMLVDHRGGAGGPPVELT
                     IPSMGAWTAGAAKLIATPKRGPGLSWAGPARYAAGGLAVLLLIGFVVNIRRKPQLQPP
                     PPQV"
BASE COUNT          606 a         1318 c         1270 g          455 t
ORIGIN      
        1 gtggcccacg ctgccatcat cgccagcaag cccggcgccg cgccacaggt ttcgctgaca
       61 ggcggaaacg ccaccgcctg gctccgccgc gaccgccgcg cgcccggcgt cgcagcccgc
      121 gccgcaggga atctcctgcc ccgccgcgag cgcgggaatg cccgaggtcg ccgtgaacgg
      181 cgcaacgtcg tgcgcccagg tcacggcgca cgtaccgccg tcggtcgatc tgtgtccagc
      241 cgacgcactc ggagcccggc gcccctgtcc acgcgcttcc acgcgcatcg atcgagaggg
      301 catcacatct gcatcccgac aggcgacgga ggacccatgg gactcgactt gcttcgagat
      361 cgcggcacgc cgatcgaacg ccagcgcttc cactggaagg agctggtcca ggagccgatc
      421 agcaagctcg acgcggacgc gctgacccgc gtccgcgtca tcctgatgaa tgggatcgaa
      481 tcggaggcca accggttcgg ccacgcggcg gcccgcctgt ttccggcatt gcaacgcccg
      541 ctcgcggtca tccgtcggat cgagcaacac cagcagaccc tcgtcaactg gctattgccg
      601 gccgaccaga ctccgctcga gaccacgatc ggctacgagc aggtcgcgat cgaggtcacg
      661 gccagcgtcg cgagcgccga gccggatccc tacatggcgg cgatcttccg gttcggactg
      721 ctcgaggact tcgatcacat gtaccggttc gcggcgctga tggatcgcac cgagggcaag
      781 gacgccagca cgctgttgca aggctacacc gacatcctgc ccgggcggcc gacgacgatc
      841 gagcaccgcc atccgctcga cgacctgcgc agtccctacg atcgccggca cgcggatctg
      901 ctgagcaagc tccacgcact caccatcatg gcaggcgaga accagaccca cgattactac
      961 atgacgatcg gcccgatgtt cgccgatccc acggcgcgcc tgctgtacgc cgagatcgcc
     1021 tcgatcgagg agcagcacgt cacacagtac gagtcgctga tcgaccctcg cgaatctgcg
     1081 ctggagaagt ggctattgca cgaggcgtgc gaagcctaca actactggag ctgcctcgaa
     1141 ttcgaggatg atcgccggat ccgcgcgatc tgggagcgca tgctcgatta cgagctagga
     1201 catgtccagg ccgtggcgcg gctgttcgag gatcacgagg gccgggatgc cgaggaggtg
     1261 ctcgaacgcc agatccgcga gccggtccag tatgccagcc atcgcgacct cgtgcgcgac
     1321 gtgctgcgcg ccgaggtcaa cctcggcgcc gatggcagcc acttgatcga tcgcacgcgc
     1381 gatcacgagc ccgagcgcac gcggcgctac cgcgccgagc tcaacgccga gatccagccg
     1441 tcgaacgtcg tcgcggccgg ctggacctgg cagcccggcg gcgagctggc cgcgcgggac
     1501 accggcaagc gctccgattc caacggcgta ccgccggagc aaggaggcaa gtcgtgaagc
     1561 gccaggtaga gagctcgacg gccccgggga agaaccacac cggcctcgcg ccgcacgcca
     1621 aggagcgcga cgacatggtg cgaggggccc aggagttcgt gccgacctcg tccggcgggg
     1681 ccgacgagat cgcccagctg cgaatcgcct acgcgcgcgc cggtggtccc cacggcacca
     1741 tgtccgcggc gtcggtggac gcggcccggt tgccggtgct cgacaagctg ggagcccgcc
     1801 tggtgttcga gcgcaccggc acccggctct acgatgcgtt gatctccaag ctcgacgcgt
     1861 atggcccgtt cgacggcggt ccgacccgcc agcagctggt cgagatccgc gaccaggagc
     1921 acgcgcatgc gctgctcgcc caggacctga tccgtgcgct cggcggcgac cccacgatgg
     1981 tgacgccgtg cggcaacctg caggcgaccg cgagccgggg catcctcgat gtgctcgtcg
     2041 atccgcgaac cagcctgatc gatggcctgg aggccatcct gatcgccgag ctcgcggatc
     2101 aggacagctg ggcagcgctc gcgcgcagcg cggaggcgct gggcgacgcc gggacgaccg
     2161 cgaagatcac gcaagcacaa cagaccgagc tccagcacct gaagaaggtg cggggctggc
     2221 tggaagcggc ggaccggctc cgcgccaagg cggccaccga agtccactga cgcttgcaca
     2281 acccgtgaag gagttaatcg acatgccgag ggaagatcgc gagtatcgca actatcgtga
     2341 cgatgacgac gaccgtcggc gcgagagtca cccgtttcgc ggcaaccagt acacgagcgg
     2401 ctcgagccgc gacgacgacc gctcgtaccg ctcgcgcggc cgggacgacg atgacgagcg
     2461 cggaggctac cgcggctcgt cgcggatgcc ggagcgcgac gactacggtc ggttcgtgag
     2521 cgacgacgat cgccgcggtg gctatcgctc gcgcgggcgc gaccgcgacg acgacgatcg
     2581 cgacatgcgc agcagcggtc gcggccgcgg cggctggttc ggtgacccgg agggccactc
     2641 cgaggcatcg cgccgcggct gggccgagcg cgacgacgat cgcggcggtg gacgccggat
     2701 gtccgaggat cgcggcggct accgcggctc gcggatgccg gagcgcgacg agcagggccg
     2761 gttcacgagc gatgacgacc gccggggccg ctcgccctac gaggatcgcg acgacgaccg
     2821 gggcggctat cgctcgcgcg ggcgcgaccg cgatgacgac gatcgcgaca tgcgccgcag
     2881 cagcgggcgc gggggctggt tcggcgatcc cgagggtcac tcggaggcgt cgcgccgcgg
     2941 ctgggagcgc ggcgaccatg gccagagcgg ctggttcggc gactcgcgcg gccacgcgga
     3001 agcatcgcgc cgcggctggg agcgcggcga ccacgcccag agcggctggt tcggcgatcc
     3061 cgaaggtcac tcggaggcgt cgcgccgcgg ctgggagcac ggccacaacg gacggggccg
     3121 gtagccgacc gacctacccg gccctgcgcc ccgtcacacc tgcggcggcg gcggctgcag
     3181 ctgcggcttg cgccggatgt tcaccacgaa cccgatcagc agcagcactg ccaggccgcc
     3241 cgccgcgtag cgcgccggcc ccgcccagct cagcccgggc ccgcgcttgg gcgtcgcgat
     3301 cagcttcgcc gcccccgcgg tccacgcccc catcgacggg atcgtcagct cgaccggcgg
     3361 gccgccggcg ccgccgcggt ggtcgaccag catcagctcg ctgcccagcc cgatcagcga
     3421 cgctccggcg gtgtgctcgt cgagagcgcg ccgcaccgcc gccagatcga ccggagccgc
     3481 gatcacgatc ccgccggcca gcccctcgcg atatcccgag atcggcatgc tcgcggccag
     3541 cgtcacgctc agcccatcgc tgcggaaccg cgtgtcgcgg ccgctcggcg gcggaagcgc
     3601 gggcgccgac ttcgggatcc ggagcagcga caccggcttg ccgttgctg
//
LOCUS       DASVOR010000799         1761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_66159,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000799 DASVOR010000000
VERSION     DASVOR010000799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1761
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66159"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /gene="groL"
                     /locus_tag="VF516_15840"
     CDS             <1..1152
                     /gene="groL"
                     /locus_tag="VF516_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830443.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX8109206.1"
                     /translation="MIAEAMKKVGKEGVITVEEAKTLTSELDVVEGMQFDRGYLSAYF
                     VTDSERMEAVLNDPYILIHEKKISNMKDLLPVLEQVAKQGKPLVIIAEDVDGEALATL
                     VVNKLRGTLHVAAVKAPGFGDRRKAMLQDIAALTGGQAVTEDLGLKLENLTLQDLGRA
                     KRISIDKDNTTIIEGAGKKQDIDARVKTIRREIEETTSDYDREKLQERLAKLVGGVAV
                     IRVGAATEVEMKEKKARVEDAMYATRAAVEEGIVPGGGVALVRCIAALDKLTFDDDRR
                     YGVNILRRALEEPLRQIANNAGVDGSIVVAKVKEGSGAFGYDAAKLEYTDLVKAGVID
                     PAKVVRTALQNASSVSGLMLTTETLIAEKPKKEDKAAGGHAGHGHDFED"
BASE COUNT          318 a          609 c          607 g          227 t
ORIGIN      
        1 atgatcgccg aggcgatgaa gaaggtcggc aaggaaggcg tgatcacggt cgaggaggcc
       61 aagacgctga cctccgagct cgacgtggtc gagggcatgc agttcgaccg cggctacctg
      121 tcggcgtact tcgtgaccga ctccgagcgg atggaggcgg tgctcaacga cccgtacatc
      181 ctgatccacg agaagaagat ctcgaacatg aaggacctgc tcccggtgct cgagcaggtc
      241 gccaagcagg gcaagccgct ggtgatcatc gccgaggacg tcgacggcga ggcgctggcc
      301 accctggtgg tcaacaagct gcgcggcacg ctgcatgtcg ccgcggtcaa ggcccccggg
      361 ttcggcgatc gccgcaaggc gatgctgcag gacatcgcgg cgctcaccgg cggccaggcg
      421 gtcaccgagg atctcggcct caagctcgag aacctgacgc tgcaggacct cggccgcgcc
      481 aagcggatct cgatcgacaa ggacaacacc acgatcatcg agggcgccgg caagaagcaa
      541 gacatcgacg cccgggtcaa gacgatccgc cgcgagatcg aggagaccac ctcggactac
      601 gaccgcgaga agctgcagga gcggctcgcc aagctggtcg gcggcgtcgc ggtgatccgc
      661 gtcggcgcgg cgaccgaggt cgagatgaag gagaagaagg cgcgcgtcga ggacgcgatg
      721 tacgcgaccc gcgcggccgt cgaggagggc atcgtccccg gcggcggcgt cgcgctggtc
      781 cgctgcatcg cggcgctcga caagctgacg ttcgatgacg accgccgcta cggcgtcaac
      841 atcctgcgcc gcgcgctcga ggagccgctg cgccagatcg ccaacaacgc cggcgtcgac
      901 ggctcgatcg tcgtcgccaa ggtcaaggag ggctcgggcg cgttcggcta cgacgcggcc
      961 aagctcgagt acaccgacct ggtcaaggcc ggcgtgatcg atccggccaa ggtcgtgcgc
     1021 accgcgctgc agaacgcgtc gtcggtgtcg ggcctgatgc tcaccaccga gacgctgatc
     1081 gccgagaagc ccaagaagga agacaaggcg gccggcggcc acgcgggcca cggccacgac
     1141 ttcgaggact gaacggcggg acggaaacgc cagagctcga tgcttcgcct cgcctaggca
     1201 gtcacatctc cgacggccca cgctgggtcc tcccccgacc ccatcccggc gtgaggccgc
     1261 ctgacgcggc gtccgcaaac accccctgcg gacgccgcct ttttttggtg cgacaccggt
     1321 cgtcgggcga tgtcgcaggc gaacgccggt cgcatccgga ctgtggcgtg cgcgtcaccc
     1381 ggcaggaccg ccgtgtcctg tcgcgccgcc gacagtgtcg acaacccatg cggggaggcc
     1441 gcctcgtgcg cggcgcaaac cgagcgatcg cccgcgcgac gcgaagcaaa tcgccggcag
     1501 cggccactca ggtcgacacg gattgccgcg gcatgggcgt tgcttcgttc gccaagcccg
     1561 gacgccatgc ctgaagcgat gaacgatcca gcggcccgtg ggtacacagc catgttgcct
     1621 cgttgcatga ctttcttcac gctgctcgcg tgggcgggca gcgccgccgc gcaggagccg
     1681 ccgccgaacc ctccgccgcc gcccaccgct ccgagccccg acgcgccggt cgcgacgatc
     1741 accggccggg tgatcgacgc g
//
LOCUS       DASVOR010000800         5452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_183430,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000800 DASVOR010000000
VERSION     DASVOR010000800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183430"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF516_15845"
     CDS             <1..459
                     /locus_tag="VF516_15845"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEX8109207.1"
                     /translation="QVHRIPAERAVPAAAATDYERALVAALGAPPRFDLVLLGMGPDG
                     HTASLFPGSPALGDTAHWVVANPVTSPLVHGTTTRITLTAPAINAARRVRFLVAGPDK
                     AAALAQVLAGPRDPTRYPSQRVAPDSGDLVWFVDQAAASALPALSPRAPA"
     gene            585..1616
                     /gene="glk"
                     /locus_tag="VF516_15850"
     CDS             585..1616
                     /gene="glk"
                     /locus_tag="VF516_15850"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEX8109208.1"
                     /translation="MILAGDIGGTKTVLALCDAAPPPGATTPGALTFTIVREQVYPCA
                     DYPSLEAVLAAFLPAGAPHGPQGVTAACFGVAGPVVDGVAQITNLPWRIDAAALAAQL
                     AVPVALLNDLQATALGALVLPATAFAVLQPETAPTRDGTIAVIAPGTGLGESLLVHDG
                     VRYRALPSEGGHADFAPGTDEEIALLHHLRGLHGDHVSYERVLSGAGIGDLYGFVRAA
                     TGTPEPAWLTAELASGDRNATISHVALAGRDPACVRALEIFVEILGAEAGNLALRGLT
                     TGGVVIGGGIPPKILPALQHRRFLDRFMAKGRFAAWMASLGVRVTLEPRAALFGAAHH
                     AATHKDARP"
     gene            1613..3217
                     /gene="zwf"
                     /locus_tag="VF516_15855"
     CDS             1613..3217
                     /gene="zwf"
                     /locus_tag="VF516_15855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX8109209.1"
                     /translation="MSEPKHPAPAAPAAPPASLSPGAASVSVTSSVTGEILGDVTTKR
                     RRPDPTAIVIFGATGDLAGRKLAPALYNLMLDSSLTDPTVIIGVSRGELTGPQFADHL
                     RPRVTEFSRRKVEPAMWDKFASTLDFVGGEFDSDATYTALKAKLDAAAARGTRGNRMF
                     YLSTPPSVFPMILQKLQQHGLIERAVQRSGKPACRVIVEKPFGRDLASARQLNEMISS
                     YLDESQIYRIDHYLGKETVQNILVFRFGNSIFEPIWNRNHVDFVEITAAESIGIEGRG
                     AFYEATGVVRDIIQNHLLQVMSLVTMEVPASFSADDIRDEKSQVLRSVRSLDLNDVAN
                     HCVRGQYRGYRDEDGVAPDSQTPTYAAMRFMIDSWRWQGVPFYLRAGKRLAERVTEVN
                     IHFRAVPLVLFKEEAAGSVLQPAMLTLRIQPHEGISLRFVAKVPGENINVGNVLMTMT
                     YADAFKRPISEAYERLLLDCMRGDATLFNRRDSVDRAWELIQPVLQIWEATPGVHLYE
                     PGSAGPAAADEMIARDGRSWRDLARS"
     gene            3282..3890
                     /locus_tag="VF516_15860"
     CDS             3282..3890
                     /locus_tag="VF516_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8109210.1"
                     /translation="MKLEYLKLTNFRGFREFEIPFDPQFTVLLGGNMAGKTAVLDGAA
                     FRLRRLLGPVQARNIEDDDVRQVVVTSGGVPLLQPEFPVLIEARTLEDRPQESSRVSV
                     AWQLYRGGANKPTENADGPYTLLNFPINKDLPVLAHFGVHRNWGRKQIADDLKGIGNR
                     YDGYTGAFDTQSAHAIMAGWIRKQPTCSYNKVMGTSSLNSPR"
     gene            3818..4549
                     /locus_tag="VF516_15865"
     CDS             3818..4549
                     /locus_tag="VF516_15865"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8109211.1"
                     /translation="MDPEATYVQLQQGNGYVQPQLAAIEAAVRSCLEGLKRFWFDVQF
                     DELRLELGNGDIRPFYMLSEGCRNMTALVADIAWRASVLNPHHGPNAHKLTEGIVLID
                     ELDLHLHPGWQRRIVADLRRTFPKIQFIVTTHSPQIVASVHRDQVRLLDRNQLVSAKP
                     YVEGRDSSEILEDVFGVPARPEEVKAEIKEVYQLIEAARFAEARARLDKLEERLGPHD
                     AELIRVRWLLDTEDNGPLASSAAST"
     gene            5004..5252
                     /locus_tag="VF516_15870"
     CDS             5004..5252
                     /locus_tag="VF516_15870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109212.1"
                     /translation="MQNDLVDKDIEALNLNAAPLVRARKAAFFAAWNDLKRANFAIGY
                     MRKLEQSLRITPSVRARPYAEFLRYHVRKKIHQKGHTP"
BASE COUNT          911 a         2069 c         1613 g          859 t
ORIGIN      
        1 caggtccacc ggatccccgc cgagcgcgcc gtccccgccg cggcggccac cgactacgag
       61 cgcgcgctgg tcgctgcgct cggcgcgccg ccgcggttcg atctcgtgct gctggggatg
      121 ggccccgacg gccacaccgc ctcgctgttc ccgggcagcc ccgcgctcgg tgacaccgcg
      181 cactgggtcg tcgccaaccc cgtcacctcg ccgctggtcc acggcaccac cacccgcatc
      241 acgctgaccg ccccggcgat caacgccgcg cgccgcgtcc ggttcctggt cgccggcccc
      301 gacaaggccg ccgcgctcgc ccaggtcctc gccgggccgc gcgatcccac gcgctatccg
      361 tcgcagcggg tcgctccgga ctccggcgat ctggtgtggt tcgtcgatca ggccgccgcc
      421 tcggcgctcc cggcgctctc tccaagggcg cccgcgtgat ccacggcgat ctcggcgtcc
      481 tgccctcctt cgcttgcgtc acgaggcccg ttgcggtccc ggggcgcccg cggcattgtg
      541 tcccggcctt gatcttgtcc tcggcctggc cctggaggtg acccgtgatc ctcgccggtg
      601 acatcggcgg caccaagacc gtgctcgccc tgtgcgacgc cgcgccaccc ccgggcgcca
      661 ccacccccgg cgcgctgacg ttcacgatcg tccgcgagca ggtctacccg tgcgccgact
      721 atccctcgct ggaggccgtg ctcgccgcgt tcctcccggc cggcgccccg cacggcccgc
      781 agggtgtgac cgccgcgtgc ttcggcgtcg ccggcccggt cgtcgacggc gtcgcccaga
      841 tcaccaacct gccctggcgg atcgacgccg ccgcgctcgc cgcccagctc gccgtcccgg
      901 tcgcgctgct caacgatctc caggccaccg cgctcggcgc gctcgtcctg cccgccaccg
      961 cgttcgccgt cctccagccc gagaccgctc ccacccgcga cggcacgatc gccgtgatcg
     1021 cccccggcac cggcctgggc gagtcgctgc tggtccacga tggcgtgcgc taccgcgccc
     1081 tgccgtccga gggcggccac gccgacttcg cccccggcac cgacgaggag atcgccctgc
     1141 tccaccacct gcgcggcctc cacggcgacc acgtcagcta cgagcgcgtg ctgtccggcg
     1201 ccggcatcgg cgacctctac ggcttcgtcc gcgccgccac cggcaccccc gagcccgcct
     1261 ggctcacggc cgagctcgcc tcgggcgacc gcaacgccac gatctcgcac gtcgccctcg
     1321 cgggccgcga tccggcgtgc gtccgcgcgc tcgagatctt cgtcgagatc ctcggcgccg
     1381 aggccggcaa cctcgcgctc cgcggcctga ccaccggcgg cgtcgtgatc ggcggcggca
     1441 tcccgcccaa gatcctcccc gcgctccagc accgccgctt cctcgaccgc ttcatggcca
     1501 agggccggtt cgccgcgtgg atggccagcc tcggcgtccg cgtcaccctc gaaccccgcg
     1561 ccgcgctgtt cggcgccgcg caccacgcag ccactcacaa ggacgcacgc ccatgagcga
     1621 acccaagcat cccgctccgg ccgccccggc cgcgccgccc gcgtcattgt cccccggcgc
     1681 ggcgtcggtg tccgtcacgt cctcggtgac cggcgagatc ctcggcgatg tcaccacgaa
     1741 gcggcgccga cccgacccga ccgcgatcgt gatcttcggc gccaccgggg acctcgccgg
     1801 ccgcaagctc gcccccgcgc tctacaacct gatgctcgac agctcgctga ccgatccgac
     1861 cgtgatcatc ggcgtcagcc gtggcgagct caccggcccg cagttcgccg accaccttcg
     1921 cccgcgggtc accgagttct cgcgccgcaa ggtcgagccc gcgatgtggg acaagttcgc
     1981 cagcacgctc gacttcgtcg gcggcgagtt cgacagcgac gccacctaca ccgcgctcaa
     2041 ggccaagctc gacgccgccg cggcccgggg cacccgcggc aaccgcatgt tctacctctc
     2101 gaccccgccg tcggtgttcc cgatgatcct ccagaagctc cagcagcacg gcctgatcga
     2161 gcgcgccgtc cagcgctccg gcaagccggc gtgccgcgtg atcgtcgaga agccgttcgg
     2221 ccgcgacctc gcctcggcgc gccagctcaa cgagatgatc agcagctatc tcgacgagtc
     2281 gcagatctac cggatcgacc actacctggg caaagagacc gtccagaaca tcctcgtgtt
     2341 ccggttcggc aactcgatct tcgagccgat ctggaaccgc aaccacgtcg acttcgtcga
     2401 gatcaccgcc gccgaatcga tcggcatcga gggccgcggc gcgttctacg aggccaccgg
     2461 cgtggtccgc gacatcatcc agaatcacct gctccaggtc atgtcgctgg tcaccatgga
     2521 ggtccctgcc tcgttctcgg ccgacgacat ccgcgacgag aagtcccagg tcctgcgctc
     2581 ggtcaggagc ctcgacctca acgacgtcgc caaccactgc gtgcgcggcc agtaccgcgg
     2641 ctatcgcgac gaggacggcg tcgcccccga ctcccagacc ccgacctacg ccgcgatgcg
     2701 cttcatgatc gacagctggc gctggcaggg cgtcccgttc tacctgcgcg ccggcaagcg
     2761 gctcgccgag cgcgtcaccg aggtcaacat ccacttccgc gccgtcccgc tcgtcctgtt
     2821 caaggaggaa gccgccggct cggtcctcca gcccgcgatg ctcaccctgc gcatccagcc
     2881 ccacgagggc atctcgctgc ggttcgtcgc caaggtcccc ggcgagaaca tcaacgtcgg
     2941 caacgtcctc atgaccatga cctacgccga cgccttcaag cgcccgatct ccgaggccta
     3001 cgagcgcctc ctgctcgact gcatgcgcgg cgacgccacc ctgttcaacc gccgcgactc
     3061 ggtcgaccgc gcctgggagc tgatccagcc cgtgctccag atctgggaag ccaccccggg
     3121 cgtccacctc tacgagcccg gctccgccgg cccggccgcc gccgacgaga tgatcgcccg
     3181 cgacggccgc tcctggcgcg acctcgcccg cagctgagcc ggcggcctac cccgggcacg
     3241 gttcgcggta agctgcgttt tagacgaacc agcccaggcc catgaagctc gagtacctca
     3301 agctgacgaa cttccgcggc ttccgcgagt tcgagatccc gttcgacccg cagttcaccg
     3361 tcctgctcgg cggcaacatg gcgggcaaga cggctgtact ggacggagca gccttcaggt
     3421 tgcgcagact tttgggaccc gtgcaggcac gcaacatcga agatgacgat gttcgtcaag
     3481 tcgttgttac cagcggtggc gtcccactcc ttcaaccaga atttccagtc ctgatcgaag
     3541 caaggactct cgaggaccgc cctcaagaga gttctagagt ttcggtcgca tggcagctat
     3601 atcgcggagg agccaacaag cccacagaga acgcagacgg tccatatacg cttctaaatt
     3661 ttccaatcaa caaagatctc ccagttcttg cacacttcgg agtacaccgc aattggggac
     3721 gtaagcagat cgctgacgat ctaaagggca taggcaatcg ctacgatggg tatacgggtg
     3781 cattcgatac tcaatccgct catgcaataa tggccggatg gatccggaag caacctacgt
     3841 gcagctacaa caaggtaatg ggtacgtcca gcctcaactc gccgcgatag aagctgcagt
     3901 caggtcctgc ctcgaagggc tgaagcggtt ctggttcgac gtacaattcg acgagcttcg
     3961 actagaactc ggcaatggag acattcgacc attctatatg ctcagcgagg gttgccgcaa
     4021 catgacggca ctggtcgcag acatcgcctg gcgagcttca gtcctcaacc cccaccacgg
     4081 tccgaatgct cacaagctta ccgaaggcat cgtattgatc gatgaactcg accttcatct
     4141 acatccaggg tggcagcgac gcatcgtggc cgacctgcgg cggacgttcc ccaagatcca
     4201 gttcatcgtc acaactcact caccacagat cgtggcgagc gtccaccgcg accaggtccg
     4261 cttgctcgat cggaaccaac tggtctcggc caagccctac gtcgagggcc gcgactccag
     4321 cgagatcctg gaggacgtgt tcggtgtgcc ggcgcgaccg gaagaagtca aggccgagat
     4381 caaggaagtc tatcagctga tcgaggccgc aaggtttgcc gaggcgcggg ctcgccttga
     4441 caagctggag gagcgacttg gccctcatga cgccgaactc atccgggtgc gatggctact
     4501 cgacaccgag gataacggtc cgctcgcgtc ctcggccgca tcgacatgat tcgttacacg
     4561 aagagcccgc caccggcggt gctgacgacg atcgctgcgg caaggccacg acgagagtgg
     4621 aacgatctcg gccgggagga ccgcgatccg atccgcgctg cgctggtccg cgaccagggc
     4681 tggctctgcg cctactgcca gagccgtatc aagcccgagg actccgctgc aggcgtcagc
     4741 cagatgaaga tcgagcactg gaccccccgc cacccagacc gagatgccgg catcgaggac
     4801 accgaccatc acttcctgtg gtccaacttg cttggcgtgt gccccggtgt tcggccgaac
     4861 ggaacgccgc ggccgatcgg aacatacctc actgcgacac gagtcgcggc aacgccaccc
     4921 tgttcctgca tcctgtcgag ggccgtggcc cggatccccg agaacacctt cagtattcca
     4981 aggatggcga ggtcgcatcg tcggtgcaga atgacctcgt cgacaaggat atcgaggcac
     5041 tgaatctcaa cgcggcacct ctggttcgcg cccgcaaggc cgcattcttc gcagcatgga
     5101 acgatctcaa gcgggccaac ttcgcgatcg gctacatgcg caagctcgag cagagcctcc
     5161 ggatcacgcc cagtgtccgc gctcgaccct acgctgagtt tctgcgttac cacgtgcgca
     5221 agaagatcca tcagaaaggg cacacgccct gaagcgcagc aacccgcggg tcgcagactt
     5281 aggttcccac aacccgcctc ggcgcaaggc cacgcgatca gtcgtcagcg atatcggcga
     5341 ccatcgtcgc cccgtgttcc atcagccgcg acaagtaacg gtacgggtcg gtcttgcggc
     5401 gctgcaccag cttcttggct gtcttcgttc caacggtgag cacgccgatc ag
//
LOCUS       DASVOR010000801         2494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_66162,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000801 DASVOR010000000
VERSION     DASVOR010000801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66162"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..1897)
                     /locus_tag="VF516_15875"
     CDS             complement(209..1897)
                     /locus_tag="VF516_15875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8109213.1"
                     /translation="MLTFSSDPDVAEQQMNAIIFYLTAFGYIDGKFDFTEKTFVRIYI
                     RQLVTARAKAAMPDADPATRDEVVTRFVAHFLEVFEQIDRSVKDLFDEAVADGEDVEK
                     FVYAKLKLRSYEIFQSFDPANQHELLTCVDELIYADGSVHPAEQAFRQELEALLDSPT
                     ALGIHDIEVMRESSRIAIEGPASLRSRLEDHPFFESFEQHYSADPARIRAQVAGDLDL
                     MRRFVAQLGEQRRAGAGRLAGKHTVAEFAGERPFLDGHVYVHPAEPGRVYELTVLGDL
                     HGCYSCLKGALLQADFFAKLEAWRLDRRQPEPKLVLLGDYIDRGRFSYNGVLRTMMQL
                     FLAAPDHVFPLRGNHEYYIEYRGRIYGGVKPAEAINTLVNHVPGEVFAEYMKLFEELP
                     SMLFFDDVLFVHAGIPRDADADKITDFAALNDPELRFQMLWSDPSTADFIPADLQAQN
                     ARFPFGKRQFEAFMNKLGCSIMVRGHEKVDAGFRPMYGEAASLITLFSAGGADNNDLP
                     EDSSYRAVTPMAATIRIDGGTVQVTPWLIDYERFNDPRRNRFFASPPEIEHKAG"
     gene            1968..>2494
                     /locus_tag="VF516_15880"
     CDS             1968..>2494
                     /locus_tag="VF516_15880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109214.1"
                     /translation="MRVWLLVAVAVCVALSGAPAARAEPHLEASGFIGVDWFGKRSSL
                     GNSWAPEQVPGTSVVFGGRIGWLVPPALPGDLRLALEAELGFAPAFTGSSVGDGRMMY
                     FAPVFEWRGQAVLRFTRWRTVVPHLVVGAGGDSVVSSSPFMADDTEPIAHWGAGLIAP
                     MTDGWQLRVDVRQGV"
BASE COUNT          348 a          823 c          926 g          397 t
ORIGIN      
        1 ccttgtcgcg gcagcgccgg accagcacga cggtcaacgg accgcggccg cgggtgtagc
       61 gcgcgccggt cccggcgtcg tgctcggcga tccgcgcggc gacgtcgcgc gcgatcccgc
      121 agtacagggt gtcgtcccgg caccgcgcga cgtagacgta ccaggccacg ccgcgagcat
      181 cgtcccgcgg cgcgccgcgc tgcaagcgtc agccggcctt gtgctcgatc tcgggcggcg
      241 acgcgaagaa ccggttgcgc cgcggatcgt tgaaccgctc gtagtcgatc agccacggcg
      301 tgacctgcac cgtgccgccg tcgatccgga tcgtcgcggc catcggggtc accgcgcggt
      361 agctcgagtc ctcgggcagg tcgttgttgt cggcgccgcc cgccgagaac agcgtgatca
      421 gcgacgccgc ctcgccgtac atcggccgga acccggcgtc gaccttctcg tggccgcgca
      481 ccatgatcga gcagccgagc ttgttcatga acgcctcgaa ctgccgcttg ccgaacggga
      541 accgcgcgtt ctgggcctgg aggtcggcgg ggatgaagtc ggcggtcgac ggatccgacc
      601 acagcatctg gaaccgcagc tcggggtcgt tgagcgccgc gaagtcggtg atcttgtcgg
      661 cgtcggcgtc gcgcgggatc ccggcgtgca cgaacaggac atcgtcgaag aacagcatgc
      721 tgggcagctc ctcgaacagc ttcatgtact cggcgaacac ctcgcccggc acgtggttga
      781 ccagcgtgtt gatcgcctcg gccggcttga ccccgccgta gatccggccg cggtactcga
      841 tgtagtactc gtggttgccg cgcagcggga acacgtggtc gggcgcggcg aggaacagct
      901 gcatcatcgt gcgcagcaca ccgttgtacg agaaccggcc gcgatcgatg tagtcgccga
      961 gcagcaccag cttgggctcg ggctggcggc ggtcgagccg ccaggcctcg agcttggcga
     1021 agaagtcggc ctggagcagc gcgcccttga ggcagctgta gcagccgtgg agatcgccga
     1081 gcacggtgag ctcgtagacc cggccgggct cggcggggtg gacgtagacg tggccgtcga
     1141 ggaacggccg ctcgcccgcg aactcggcga ccgtgtgctt gccggcgagc cggccggcgc
     1201 cggcgcggcg ctgctcgccg agctgggcga cgaaccgccg catcaggtcg agatcgccgg
     1261 cgacctgggc gcggatccgc gccgggtcgg cggagtagtg ctgctcgaag ctctcgaaga
     1321 acgggtgatc ctccagccgc gagcggagcg acgccgggcc ctcgatcgcg atccggctcg
     1381 actcgcgcat gacctcgatg tcgtggatgc cgagcgcggt cggcgagtcg agcagcgcct
     1441 cgagctcctg gcggaacgcc tgctcggcgg ggtggacgct gccgtcggcg tagatcagct
     1501 cgtcgacgca ggtcagtagc tcgtgctggt tcgccgggtc gaagctctgg aagatctcgt
     1561 agctccggag cttgagcttg gcgtagacga acttctcgac gtcctcgccg tcggcgaccg
     1621 cctcgtcgaa caggtccttg acgctgcggt cgatctgctc gaagacctcc aggaagtgcg
     1681 ccacgaaccg ggtgacgacc tcgtcgcggg tcgccggatc ggcgtcgggc atcgccgcct
     1741 tggcgcgcgc ggtcaccagc tggcggatgt agatccggac gaacgtcttc tcggtgaagt
     1801 cgaacttgcc gtcgatgtac ccgaacgcgg tcaggtagaa gatgatcgcg ttcatctgct
     1861 gctcggcgac gtccggatcg ctgctgaagg tgagcatcgg cgaggctagc ttgccccggc
     1921 cgtgattgcc cgtgcaagcg ccgagacagc gctgatagga tggagcggtg cgcgtctggt
     1981 tgctcgtcgc cgtcgcggtt tgcgtcgccc tgagcggcgc accggcggcg cgcgccgagc
     2041 ctcatctcga ggcgagcggg ttcatcggcg tcgactggtt cggcaagcgc agctcgctcg
     2101 gcaactcgtg ggcgcccgag caggtgcccg ggacgtcggt ggtgttcggc gggcggatcg
     2161 gctggctcgt gccgccggcg ctgccgggcg atctgcggct cgcgctcgag gccgagctcg
     2221 ggttcgcacc ggcgttcacc gggtcgagcg tgggcgacgg ccggatgatg tacttcgcgc
     2281 cggtgttcga gtggcgcggc caggcggtgc tgcggttcac ccggtggcgc accgtggtgc
     2341 cccacctcgt ggtcggcgcc ggcggcgaca gcgtggtgtc gagctcgccg ttcatggccg
     2401 acgacaccga gccgatcgcg cactggggcg ccggcctgat cgcgccgatg accgacggct
     2461 ggcagctgcg ggtcgacgtt cgccaggggg tgat
//
LOCUS       DASVOR010000802         4870 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_37009,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000802 DASVOR010000000
VERSION     DASVOR010000802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37009"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3828)
                     /locus_tag="VF516_15885"
     CDS             complement(<1..3828)
                     /locus_tag="VF516_15885"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8109215.1"
                     /translation="MRASEQPGATPGEPVRASASLDQWSGARMPVQQFLPLAVRAAGE
                     LAALHARGAIHQNIRPSSILFDAATGELAFVSPSIAEWTTPTLSEGSLPYISPEQTGQ
                     MNRPIDSRSDLYSLGVVFYQLLAGRLPFEAKDAVGWMHCHVAHQPRPLDQARPSVPRP
                     VMDIVGKLLAKLPDHRYQSALGLRHDLERCLREWSEGGALAAFPLGERDVSEEFRIPQ
                     QLYGRNVESAVLRESFERAAESGIPELVLVSGYAGIGKSAMVREMLRTVVRQRGWHIA
                     GKFEQYKRDIPYFAIIQALRELALHILTESKPRIAKRRQQLAHALGPHGKLVVDLVPQ
                     LGLIVGPQPPVPELSLTDSETRLRLVFGRLFAACATPEHPLAMFIDDMQWADTASLAL
                     VANLLTDSDTRHLLIIGAYRDNEVDPPHPLVHMLEALRCGDARIRDLVLGPLSEEDLG
                     RLVADTVHASPAEAAPLASLVRDKTGGNPFFAIQFLTALYHKRAIWFDREAHRWRWDA
                     ARIRTEGYTDNIAELMRGRLYALPSETQAALQRAACISGTVDDVTLAIACEREVAAVL
                     RPAIEQHLLFETVHAERRTYRFPHDRVHEAAYALVPQPERAKVHLEIGWRLLASTTPE
                     DLPGKVFEIVSQLDRGAALIESEHDRERLAELYLLAGSRAQASTAYASALHYFTAGAE
                     LLAKEPEVRRPELTFALDLHRAECEFLTRAFDASEQRLAGLTRRAAGLVDLAAVTSAR
                     IELYLMIDQGVLGVEAILEYLRRAGTEWPVHPTDDEVRREYERIWQQLGPRAIEDLVD
                     LPSMTDPEYRATTDLLTLAAPVALFTDENLYALMVCRVVNLSLEHGNSDASCMGYVAL
                     GVILGMRLGNPAAGFRFGQVGLDLVERRGPLRWKARVYGEFAHRILPWTKHPRDGIEL
                     ARRAFDAAIETGDLTFAAYTCSSRCTLAWLAGDPLAAVLPEADAALAFMQKLKFGMMV
                     DAIGVYRQLIRSLRGMTRELGSFDDEEFSEDRFEQHLGDEPRFAACWYWIHKVQARFW
                     AGDHAGAVAAAARARPMLWAMPAFSEVAEQVFYAALAYAAHYNAAPEDERPRLRDELA
                     ARQAQIAAWAEHGPDRFRDRAELTGAELARIRGEHDQAARRYEQAIRSARAGGYVQIE
                     ALAYEVAARFHRARGQALIADAYVREAYIRYVRWGAEGKARQLRHAHPELELQPAGSA
                     TVALRPEQLDQLSVIKALQTISSVMDKDLLSRTLLRFLLEKGGAQRVVLVTSRDGELE
                     IAAEARVDEPSAAE"
     gene            complement(3815..4294)
                     /locus_tag="VF516_15890"
     CDS             complement(3815..4294)
                     /locus_tag="VF516_15890"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8109216.1"
                     /translation="MFATAAYAGIEPRRSAPPRVQPSCFVTGDRKEVDTSRFVLIVDD
                     DPDLLDVTSFVVENEGIAVETARNGKEALALLATGRLPALVLLDMMMPVMNGWEFLAA
                     VANERLFKGIPIVVLTAAEHAEVLGAMEVLSKPTDLKELIRVVERYVRKDGDKDAGV"
BASE COUNT          738 a         1687 c         1690 g          755 t
ORIGIN      
        1 ctccgctgcg gagggctcgt cgaccctcgc ctcggccgcg atctcgagct cgccgtcgcg
       61 tgaggtgacc aggaccacgc gttgtgcgcc gcccttctcc agcaggaacc gcagcagcgt
      121 gcgtgacagc aggtccttgt ccatgacgct ggagatcgtc tgcaacgcct tgatcaccga
      181 cagctggtcg agctgctcgg ggcgcagcgc cacggtcgcc gagccggccg gctggagctc
      241 gagctcgggg tgggcgtggc ggagctgtcg cgccttgccc tcggctcccc accggacgta
      301 gcggatatag gcctcgcgca cgtaggcgtc ggcgatcagc gcctggcccc gcgcgcgatg
      361 aaaccgcgcc gccacctcgt aggcgagcgc ttcgatctgg acgtaaccgc ccgcgcgcgc
      421 gctgcggatc gcctgctcgt agcgccgggc tgcctggtcg tgctcgccgc gaatgcgcgc
      481 gagctcggcg ccggtgagct cggcgcgatc gcggaagcga tcggggccat gctcggccca
      541 cgccgcgatc tgtgcctggc gggcagcgag ctcgtcgcgc agccgtggcc gctcgtcctc
      601 gggcgcagcg ttgtagtggg cggcatacgc gagcgcggcg tagaacactt gctccgcgac
      661 ctcggagaac gctggcatcg cccacagcat cggccgtgcc ctggctgcag ccgccaccgc
      721 gccggcatga tcgccggccc agaaccgcgc ctgcaccttg tggatccagt accagcacgc
      781 cgcgaagcgc ggttcgtccc cgaggtgctg ctcgaaccga tcttcggaga actcctcgtc
      841 gtcgaacgag ccgagctccc gtgtcatccc tcggagcgac cggatcagct gccggtacac
      901 cccgatggcg tcgaccatca tgccgaactt gagcttctgc atgaacgcga gtgcggcatc
      961 ggcctcggga aggacagccg cgagcggatc gcctgccagc caggcgagcg tgcaccggct
     1021 gctgcaggtg tacgccgcga acgtgagatc gccggtctcg atcgctgcgt caaaggcgcg
     1081 ccgcgccagc tcgatgccat cgcgcgggtg cttcgtccac ggcaggatcc ggtgggcgaa
     1141 ttcgccgtag acgcgcgcct tccagcgcag cggcccgcgc cgctccacca aatccaaccc
     1201 gacctggccg aaccggaacc ccgctgcggg gttgcccaac cgcattccga ggatcacacc
     1261 gagcgcgaca tagcccatgc acgaggcgtc gctgttgccg tgctcgaggc tcaggttcac
     1321 cactcgacag accatgagcg cgtacaggtt ctcgtcagtg aacagcgcaa ccggcgccgc
     1381 gagcgtgagc agatccgtcg tcgcgcggta ctccggatcg gtcatcgacg gtaggtcgac
     1441 gagatcctcg atcgcgcgcg ggccgagctg ctgccagatc cgctcgtact cgcgccggac
     1501 ctcgtcgtcg gtcggatgca ctggccactc ggtgcccgcg cgccgcagat actcgagtat
     1561 cgcctcgacg ccgagcacgc cctgatcgat catcaagtag agctcgatcc gcgccgacgt
     1621 gaccgctgcc aggtcgacga gacctgcggc gcggcgcgtg agcccggcga gtcgttgctc
     1681 ggacgcgtcg aatgcgcggg tcaggaactc gcactcggcg cggtggagat cgagcgcgaa
     1741 cgtgagctcg gggcggcgca cctcgggctc cttcgcgagc agctcggcgc ccgcggtgaa
     1801 gtagtggagc gccgacgcgt aggccgtcga ggcctgcgcg cgcgagccgg cgagcagata
     1861 cagctcggcg agccgctcgc ggtcgtgctc cgactcgatc agcgcggcac cacgatcgag
     1921 ctggctcacg atctcgaata ccttcccggg aaggtcctcc ggtgtcgtgc tcgcaaggag
     1981 ccgccagccg atctccaggt ggacctttgc gcgctccggc tggggcacga gcgcataggc
     2041 ggcctcgtgc acgcgatcgt gggggaaccg gtaggtgcgc cgctcggcgt ggacggtctc
     2101 gaacagcagg tgctgctcga tcgcggggcg tagcaccgct gcgacctcgc gttcacacgc
     2161 gatcgccagc gtgacgtcgt cgaccgtgcc gctgatgcac gccgcccgct ggagcgcggc
     2221 ctgggtttcg ctcggcagcg cgtacagccg gccgcgcatc agctcggcga tgttgtcggt
     2281 gtagccctcg gtgcggatcc gggcggcgtc ccatcgccag cggtgcgctt cgcgatcaaa
     2341 ccagatcgcc cgcttgtggt agagcgcggt caggaactgg atcgcgaaga acgggttgcc
     2401 gccggtcttg tcgcggacga ggctcgcgag cggcgccgcc tcggcgggcg atgcatggac
     2461 cgtgtcggcg acgagccggc cgagatcctc ctccgacagc ggtccgagca cgaggtcgcg
     2521 aatccgcgcg tcgccgcacc gcagcgcttc gagcatgtgc accagcgggt gaggcggatc
     2581 gacctcgttg tcgcgatacg cgccgatgat cagcaggtgg cgggtatcgc tgtcggtcaa
     2641 caggttagcg acgagggcaa ggctcgcggt gtccgcccat tgcatgtcgt cgatgaacat
     2701 cgcgagcggg tgctcggggg tggcgcacgc cgcgaacagc ctgccgaaca ccaggcggag
     2761 ccgcgtctcc gagtcggtca gcgagagctc gggcacgggc ggttgcggtc cgacgatcag
     2821 cccgagctgc gggaccagat ccacgaccag cttgccgtgc ggtccgaggg cgtgcgcgag
     2881 ctgctgccgc cgcttcgcga tccgaggctt gctttccgtc aggatgtgga gcgcgagctc
     2941 gcggagcgcc tggatgatcg cgaagtacgg gatgtcgcgc ttgtactgct cgaacttgcc
     3001 tgcgatgtgc cacccgcgct gcctcacgac cgtgcgcagc atctcgcgca ccatcgccga
     3061 cttgccgatg cccgcgtacc ccgacaccag gaccagctcc ggtatcccgg actcggcggc
     3121 gcgctcgaac gactcgcgca gcaccgcgga ctcgacgttg cggccgtaca gctgctgcgg
     3181 gatccggaac tcctcggaga cgtcccgctc gccgagcggg aatgccgcga gcgccccgcc
     3241 ctcgctccac tcgcgcaagc agcgctccag atcgtggcgc agccccagcg cactctgata
     3301 gcgatggtcg ggtagcttcg cgagcagctt gccgacgatg tccatcaccg ggcgtggcac
     3361 cgatggtcgc gcctggtcga gcggccgtgg ctggtgcgcc acgtggcaat gcatccatcc
     3421 caccgcgtcc ttggcttcga acggtaaccg gccggcgagc agctggtaga acaccacgcc
     3481 gagcgagtac agatccgagc ggctgtcgat cggccgattc atctgcccgg tctgctccgg
     3541 tgagatgtac ggcagcgagc cctcgctcag cgtgggggtc gtccactccg cgatcgaggg
     3601 actcacgaac gcaagctccc ccgtggccgc atcgaacaga atgctggagg gcctgatgtt
     3661 ctggtggatc gcaccgcgcg cgtgaagcgc ggcgagctcg cccgccgcgc ggaccgccag
     3721 cggcaggaat tgctggaccg gcatccgcgc accgctccac tgatcgagcg aggcgctcgc
     3781 gcgcactggc tctcccggcg ttgcgccggg ctgctcagac gcccgcatcc ttgtctccgt
     3841 ctttgcgaac gtagcgctcg acgacccgga tcagctcctt gagatccgtt ggcttgctca
     3901 ggacttccat cgccccgaga acctccgcat gctcggccgc cgtcaacacc acgatcggga
     3961 tccccttgaa caatcgttcg ttcgcgaccg cggccaggaa ctcccagccg ttcatcacgg
     4021 gcatcatcat atcgaggagc acgagcgccg gcagcctgcc ggtggccagc agcgcgagcg
     4081 cttccttgcc gttcctcgcg gtctccacgg ctatcccctc gttctcgacc acgaagctcg
     4141 tcacgtcgag gagatccgga tcgtcatcga cgatgaggac gaacctggat gtgtcgacct
     4201 ccttccggtc gcccgtcacg aagcagctcg gttgcacccg cgggggcgcc gaccggcggg
     4261 gctcgattcc tgcgtatgcc gcggtcgcga acatcgccac caggctacct ccatcatgat
     4321 gctcgcgtac gtggaggtag acctcgcgtg accagcaagt gagctttgag tgagagagta
     4381 tctgaactcc aggtggtatt ccgacgagac aaggagatcc gggcgatgtc catcgtctcg
     4441 gccggcgcgt cgctcgtggg ctcgagcgcg cgcaggatct cctcgtagtc gccggtccgg
     4501 aacgcgtggc agcatcgggt gggagatcac gggcgtgcgc ccgatggtcg tgccgtcgaa
     4561 gcggaacagc aggccgggcg cgagatcgcc gaccggccgg atctggaacg gccggttcgt
     4621 ggtcgcgtag gtcggcggct cgcaggcgtg ctgcgggacc tcggcgacca gcaccggtga
     4681 ggtacagccg gcggtgaaca cccgcgtcgc ggtggtgacg gcgaccggaa tgctgcggat
     4741 cgcaccgcgc aggctgcgcg gccggcagtc ggcgcccagc gcggtgtcga acagccgatc
     4801 gtcgagcacg cggagccggg tggcggggtc ggtggcggcc gccgggccgg cgccggggtc
     4861 ggcgcgagat
//
LOCUS       DASVOR010000803         4986 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387490,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000803 DASVOR010000000
VERSION     DASVOR010000803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4986)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4986)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4986
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387490"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..357)
                     /locus_tag="VF516_15895"
     CDS             complement(<1..357)
                     /locus_tag="VF516_15895"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8109217.1"
                     /translation="MSTVTLNNGVEMPAIGLGVFQTPPEETRAAVEAALATGYRHIDT
                     AAAYGNERQVGEAVLKSGVDRSDVFLETKIWISDYGYDQTLHGYEKSAGKLGVDRIDL
                     LILHQALPSEFDKTLEA"
     gene            complement(382..1215)
                     /locus_tag="VF516_15900"
     CDS             complement(382..1215)
                     /locus_tag="VF516_15900"
                     /inference="COORDINATES: protein motif:HMM:NF004824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEX8109218.1"
                     /translation="MSTWLITGSSSGLGRDLARAVLEAGHNAVLTARDPGSVKDLADA
                     HPDTALAVALDVTDPAQVTQAVRAGEERFGAVDVLVNNAGYGYRAAVEEGDDADVATL
                     FATNFFGPVALIKAVLPGMRARRHGAIVNISSIGARICPAGSGYYSASKSALEGMSGA
                     LRKELAPLGISVTVVEPGAFRTDFAGRSLQQSTTVIDDYAETAGKRRKEVDTAHGAQP
                     GDPARAAQAIITAVEADEPPALLILGPDALNAIRGVIDAQLVELTDWENTSLGTDLRS
                     F"
     gene            complement(1220..1990)
                     /locus_tag="VF516_15905"
     CDS             complement(1220..1990)
                     /locus_tag="VF516_15905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8109219.1"
                     /translation="MGIDRSIARRRGSMTLMNQARVAVITGASSGIGAATARALAADG
                     HRVALLARRVDRIKALADELGHGALAIEADVTDRDALVAAADRVRQEFGRLDALVNNA
                     GIMLLGPFSAEQREDYRRMVEVNLLGAITTTEVFLDQLKDGGGDIVNISSVAGRTARA
                     GNGVYAATKWGLNGWSESLRRELLPDVRVTLIEPGAVATELPTHITHPETRQGTQQLY
                     DQVEVTAEDVAEVIAFAVSRPRRLAIHEILLRPAGQEL"
     gene            2083..2955
                     /locus_tag="VF516_15910"
     CDS             2083..2955
                     /locus_tag="VF516_15910"
                     /inference="COORDINATES: protein motif:HMM:NF037627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8109220.1"
                     /translation="MDNQVEVRDFLRTRRARVSPQQAGVIGGSRRRVTGLRREEVATL
                     AGVSTDYYAKMERGALTGVSPEILDAVARALQLDEAETEHLHDLARAAGPTPRRTRNQ
                     PATPTIRPSLQRFLDAITGAPAWITNPRRDTLATNALGRALLAPMLDDPASGGGNNAR
                     FTFLSPAARIFYPDWEQAADSTVASMRSAAGRNPHDKPLTDIIGELVTRSDEFRHRWS
                     AHNVRFHRAGTKRIHHPDVGDLEFSYEGLELPDSPGLVLYAYTAVAGSPTEERLQLLG
                     SLAATRTTPSTQDN"
     gene            2982..3296
                     /locus_tag="VF516_15915"
     CDS             2982..3296
                     /locus_tag="VF516_15915"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8109221.1"
                     /translation="MTEPTPAQRAIGGFAPKLVDLTDDVLFGDVWERSELGKRDRSLA
                     TVASLVTSGAFEQLPNHLRLAKQNGLTETELIEAIVHLAFYAGWPKAMSAIPVAKKVF
                     AE"
     gene            3437..3733
                     /locus_tag="VF516_15920"
     CDS             3437..3733
                     /locus_tag="VF516_15920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109222.1"
                     /translation="MFEVAGLAGAVGVKGGGEAADQTRDEDRVEVPEVVGAHLVGLEM
                     DGGELVGAEGAAGEDEQELVAFLLSRSPLDDQERADGRLDSEFFVDLADARCQG"
     gene            4609..>4986
                     /locus_tag="VF516_15925"
     CDS             4609..>4986
                     /locus_tag="VF516_15925"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8109223.1"
                     /translation="MIFVSYAKKDTSVASSVLSRMAAEDLEVFDWQGERRGDDFIDKI
                     EGKIREATSFVALMSPNFLASTWCRRERNLALIRDNDARRRTGAPFLQILKISDVPQA
                     DAGFLQPYDWFDMGRLDDLVTALR"
BASE COUNT          772 a         1679 c         1769 g          766 t
ORIGIN      
        1 ggcctccaga gtcttgtcga actccgacgg gagcgcctgg tgcaggatca gcaggtcgat
       61 ccggtcgacg ccgagtttgc cggcgctctt ctcgtacccg tgcagcgtct ggtcgtagcc
      121 gtagtcgctg atccagatct tggtctccag gaagacgtcg gatcggtcga cgcccgactt
      181 caggacggcc tcgccgacct gccgctcgtt gccgtaggcc gccgcagtgt cgatgtgccg
      241 gtagccggtc gcgagcgcgg cctcgacggc ggcgcgggtc tcctccggcg gggtctggaa
      301 gacgccgagg ccgatggcgg gcatctcgac gccgttgttc agagtcacgg tagacatgag
      361 tgattccttc tggtcaggaa ttcagaagct gcggaggtcg gtgccgaggc tggtgttctc
      421 ccagtccgtc agctcgacga gctgggcgtc gatgacgccg cggatggcgt tcagtgcgtc
      481 ggggccgagg atcagcaggg cgggcggctc gtcggcctcg acagcggtga tgatcgcttg
      541 ggcggcgcgg gccgggtcgc cgggctgggc gccgtgggcg gtgtcgacct ccttgcggcg
      601 cttgccggcg gtctcggcgt agtcgtcgat gacggtggtg gactgctgca gggatcggcc
      661 ggcgaagtcg gtgcggaagg cacccggctc gacgacggtg acgctgatgc cgagcggggc
      721 gagttccttg cgtagcgccc cggacatgcc ctccagggcg gacttgctcg ccgagtagta
      781 gccggatccg gccgggcaga tccgcgcgcc gatggaggag atgttgacga tggcgccgtg
      841 ccggcgggcg cgcatgcccg gcaggacggc cttgatcagc gcgaccgggc cgaagaagtt
      901 ggtcgcgaac agggtggcga cgtcggcgtc gtcgccctcc tcgacggcgg cgcggtagcc
      961 gtacccggcg ttgttgacca ggacgtcgac cgcgccgaag cgctcctcgc cggcccggac
     1021 ggcctgagtc acttgggcgg ggtcggtgac gtcgagcgcg acggctaggg cggtgtcggg
     1081 gtgggcgtcg gcgaggtcct tgacggagcc ggggtcgcgg gcggtgagga cggcgttgtg
     1141 cccggcctcg agtacggccc gggccaggtc gcggccgagg ccggacgagc tgccggtgat
     1201 cagccaggtg gacatggtgt cagagctcct gtccggccgg gcggagaagg atttcgtgga
     1261 tggccagccg ccgcggccgg ctgacggcga aggcgatgac ctcggcgacg tcctcggcgg
     1321 tgacctcgac ctggtcgtag agctgctggg tgccctggcg cgtttcgggg tgggtgatgt
     1381 gggtgggtag ttcggtcgcg acggcgccgg gctcgatgag ggtgacccgg acgtcgggca
     1441 gcagttcgcg ccgcagcgac tccgaccagc cgttgaggcc ccatttggtg gccgcgtaca
     1501 caccgtttcc tgcgcgggcg gtgcgaccgg cgacggagga gatgttcacg atgtccccgc
     1561 cgccgtcctt gagctggtcg aggaacacct cggtggtggt gatcgcaccg aggaggttga
     1621 cctcgaccat gcgccggtag tcctcacgct gctccgcgga gaatgggccg agcagcatga
     1681 tgccggcgtt gttgactagc gcgtcgaggc ggccgaactc ctgccggacc cggtcggcgg
     1741 cggcgacgag ggcgtcccgg tcggtcacgt cggcctcgat cgcgagtgcg ccgtgcccga
     1801 gctcgtcggc cagcgccttg atccggtcga cccggcgagc gagcagcgcc acgcggtggc
     1861 cgtcggcggc gagcgcacgt gccgtggcgg cgccgatccc ggaggaggcg ccggtgatga
     1921 cggcgacgcg agcctggttc atgagagtca tggatcctct tcgtcgggcg atgcttcggt
     1981 cgatgcccat tccactcgct tagatccgcg cctggggcgg cgggtctatc cgtgtactgg
     2041 cagtccaccc caaccgcgac cggcgcctcg tacggtgacg gcgtggacaa ccaggtcgag
     2101 gtacgcgact tcctccggac ccgccgcgca cgcgtcagcc cgcagcaggc aggggtcatc
     2161 ggtggcagcc ggcgtcgggt caccggcctg cgccgcgagg aagtcgccac gctcgccggg
     2221 gtgagcaccg actactacgc caagatggaa cgcggcgccc tgaccggggt ctcacccgaa
     2281 atcctcgacg cggtcgcccg tgccctccaa ctggacgagg ccgagaccga gcacctgcac
     2341 gacctggccc gcgccgccgg ccccacgccc cgtcggactc ggaaccagcc ggccacgccg
     2401 accatacggc cgtcactgca acggttcctc gacgccatca ccggcgcacc cgcctggatc
     2461 acgaacccac ggcgggacac cctggccacc aatgcgctcg gccgggcgtt gctcgcgccg
     2521 atgctcgacg acccggccag cggcggcggc aacaacgccc ggttcacctt tctcagcccc
     2581 gccgcccgca tcttctaccc ggactgggag caggccgcag acagcaccgt cgcctcgatg
     2641 cgctccgccg ccggccgcaa cccgcacgac aagccgctga ccgacatcat cggcgagttg
     2701 gtcacccgct ccgacgaatt ccggcaccgg tggtccgcgc acaacgtccg cttccaccgc
     2761 gccggcacca aacggatcca tcaccccgac gtcggcgacc tcgaattcag ctacgagggc
     2821 ctcgaactac ccgacagccc cggactggtg ctgtacgcgt acacggccgt cgccggctct
     2881 cccaccgagg agcgcctgca gctgctcggc agcctcgccg caacccgcac cacaccgtcc
     2941 actcaggaca actgaccgcg cctcggagga agccaccagc catgaccgaa ccgacgcctg
     3001 cgcagcgagc gatcggcggc ttcgcgccca agctcgtcga cctcaccgac gacgttctgt
     3061 tcggtgacgt ctgggaacgc tccgagctcg gcaagcgcga ccgcagcctc gccaccgtcg
     3121 ccagcctcgt cacctcgggc gccttcgagc aactgcccaa ccacctacgc ctcgccaagc
     3181 agaacggcct caccgagacc gagctcatcg aggcgatcgt gcacctggcc ttctacgcgg
     3241 gctggccgaa agccatgtcc gccatcccgg tcgccaagaa ggtcttcgcc gaataggcag
     3301 ctggcggcgc gccgtctctg agggccgcgc gaccgaggac ggcctaaggc cgcccgcggt
     3361 gtcgcgcggg cccggccgga cggccagctt gatccaagat cggccaattc ggcagcggag
     3421 cggctggtgt ggcgccgtgt tcgaggtggc gggtctggcc ggcgcggtag gcgtcaaggg
     3481 cggcggcgag gcggcggacc agacgcggga tgaggaccgc gtcgaggtcc cggaagtcgt
     3541 aggagcgcac ctcgtcggcc tggagatgga tggcggcgag ctggtcggcg ctgagggtgc
     3601 cgccggcgaa gatgaacagg agcttgtcgc cttcctgctc agccggagcc cactcgatga
     3661 ccaggagcgg gccgatgggc ggctcgattc cgagttcttc gtggacctcg cggacgcacg
     3721 ctgccagggg tgactcgccc ggctcggcgt aacggccggg gaccgatctc tccgttgcgg
     3781 gatcggggcc ggccgttcga gacgccggac gcgcgcctcg agcgcgtcgg tcgagccggc
     3841 gtcgaccatg ccgcgaccgg tcgagggcgc cgcgatccgc cagccatgtg cagccatgcc
     3901 gcgcatggcc agggcgacga cgagtcaatt ggtggaccaa gatcgtctcg aaggttaata
     3961 gcgatcttgc tcagtacaga ataggtggtc tgagctgcgc aaatagatgc gcgctcggca
     4021 ggactcgaac ctgcaacctc ttgatccgta gtggcctgcg gttcatctaa cctgcaggtt
     4081 tgtggtcgcg gctcaggttt ctctggcgtg ctcgcgctca tgacaggcac tggacggcac
     4141 cagttcgtgg accacttggt ggaccaagat cccttcggtc cggctgcctg catcttccgg
     4201 tctgcaggaa ccgccccaca gccaggccga cgtgagcggc tgcagggcag tacagccaga
     4261 ggcatgctgc tagcgacggg caggagatcc ttcgccgctt caagaccctg caccgtactt
     4321 ctcgaccgtg gagtcgaccg caatatggcg acagcacgat cgccatcacc tgttcggggg
     4381 catcaggagc ggcggccacc ccaccaggcg catccttcgg acaagcgctt ttccagactg
     4441 tagtgtggcg ccgcgcgtcg gcagggatga cactagtgct gggcaactcc gggaggcgcc
     4501 tctggccgcg acaacagaaa ggccgccggg tcaggccgag acgccggcag ccgacagtta
     4561 cgacgtcggg acggccgcat ccggggcgcg cggggagcac agagccaagt gattttcgtc
     4621 agctacgcca agaaggacac atcggtcgct agcagcgtgc tcagccggat ggcggcggag
     4681 gatcttgagg tgttcgactg gcaaggtgag cgacgcggcg acgacttcat agacaagatc
     4741 gagggcaaga tccgcgaggc cacgtccttc gtcgctctca tgtcccccaa cttcctcgcg
     4801 tcgacatggt gccgccgtga acgcaacctt gcactgatac gtgacaacga cgcgcgccgc
     4861 cggacgggcg caccgttcct ccagattctg aagatcagcg acgtgccaca ggcggacgcc
     4921 ggcttcctgc agccgtacga ctggttcgat atgggacgcc ttgacgactt ggtgacggcc
     4981 ctgcgc
//
LOCUS       DASVOR010000804         2293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_270616,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000804 DASVOR010000000
VERSION     DASVOR010000804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270616"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..352)
                     /gene="rpmE"
                     /locus_tag="VF516_15930"
     CDS             complement(143..352)
                     /gene="rpmE"
                     /locus_tag="VF516_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEX8109224.1"
                     /translation="MKDTAGHPHYRPAHITCACGAVHDTFSTRGDFGVDICSNCHPFF
                     TGKQKLLDTAGRIDRFNKRYAKKTS"
     gene            complement(505..1374)
                     /locus_tag="VF516_15935"
     CDS             complement(505..1374)
                     /locus_tag="VF516_15935"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8109225.1"
                     /translation="MARVLRLPDHGRLLVCTDLQGCLRDFQRFVQIFEQALIDHHGDA
                     HVLFTGDLIHGPHIDPEDWPDFLGEYYRDASGEVMMGFAELSARYPGRVHALLGNHEH
                     GHIGGPHTAKFAADEVALLEQILGPAATARMRSIIQTFALAAVTRCGAVFTHGAPAAQ
                     IDSLAELEAADLSGANYASPLDVLDTPVVGKILWARSATDAEARRFLRAVNGTIAIYG
                     HDVIPEGYEINGDTQMVVSTSFGVFDTNKVYVSLDLGAKYRVIHDLRIGHEVLPLYPD
                     RAPPRLGGRARPN"
     gene            1484..1747
                     /locus_tag="VF516_15940"
     CDS             1484..1747
                     /locus_tag="VF516_15940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEX8109226.1"
                     /translation="MRANVTGRVQGVAYRASTVEQARVHGVTGWVRNLPDGSVELEAQ
                     GDEARVSALLAWCRHGPPAARVAGVTVEDREIVAGERDFVQRR"
     gene            complement(1777..>2293)
                     /locus_tag="VF516_15945"
     CDS             complement(1777..>2293)
                     /locus_tag="VF516_15945"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8109227.1"
                     /translation="AGLELATSSEADYDKLDDARKAELLDQLELDEATARMQWRRGDQ
                     AVLATLGLRPAGIAWAARGPIEAPELGRELRMAKYDAYIHSVYVAPAARGRAVAPLML
                     EHLTKELRATDAYRSWALIAADNHASLRAFQKASFTPVCDVIHAKMATVDRVVCRPPD
                     PEAQELLGLTS"
BASE COUNT          313 a          747 c          879 g          354 t
ORIGIN      
        1 cgcgcttgtt gatgacctcc gcggtgccga gcagggcgcc gagctcctcg tggcggcgct
       61 cgagcgtcgc catcttgtcg cggaagttct gggtgtcgaa catggcacgg gcctcgcgca
      121 gggcgccgcc gccggccggg ggttacgaag tcttcttcgc gtaccgcttg ttgaaccggt
      181 cgatccggcc ggcggtgtcg agcagcttct gcttgccggt gaagaacgga tggcagttcg
      241 agcagatgtc gacgccgaag tcgccgcggg tggagaacgt gtcgtggacc gcgccgcagg
      301 cgcacgtgat gtgcgcgggg cggtagtggg gatgacctgc tgtgtctttc atgggatacc
      361 tcgttacccg gccggggccg ggggctcgcg gtgagcccgt atctataggt acggcgaccg
      421 ccgaaagcaa gccggccggg gcggtgtccg gcggcccggg ggctcccagg cgacctgaaa
      481 tcagggactt gctatccgtg acggtcagtt ggggcgggcc cgaccgccga gccggggcgg
      541 cgcccggtcg gggtagaggg gcaggacctc gtggccgatc cgcaggtcgt ggatcacgcg
      601 gtacttcgcg ccgaggtcga ggctgacgta gaccttgttg gtgtcgaaca cgccgaagct
      661 ggtcgagacc accatctggg tgtcgccgtt gatctcgtag ccctcgggga tgacgtcgtg
      721 gccgtagatc gcgatcgtgc cgttcaccgc gcgcaggaac cgccgcgcct cggcgtcggt
      781 cgccgagcgc gcccacagga tcttgccgac cacgggagtg tcgagcacgt cgagcgggct
      841 ggcgtagttt gcgccggaca ggtcggcggc ctcgagctcg gcgagcgagt cgatctgggc
      901 cgccggcgcg ccgtgggtga acaccgcgcc gcaccgggtg accgcggcga gcgcgaaggt
      961 ctggatgatg ctgcgcatcc gcgcggtcgc ggccgggccg aggatctgct cgagcagcgc
     1021 gacctcgtcg gccgcgaact tggcggtgtg cggcccgccg atgtggccgt gctcgtggtt
     1081 gccgagcagc gcgtggacgc gacccgggta gcgcgccgac agctcggcga agcccatcat
     1141 gacctcgccg ctcgcgtcgc ggtagtactc gcccaggaag tcgggccagt cctcgggatc
     1201 gatgtgcggg ccgtggatca ggtcgccggt gaacaggacg tgggcgtcgc cgtgatggtc
     1261 gatcagcgcc tgctcgaaga tctggacgaa gcgctggaaa tcgcgcaggc aaccctgcag
     1321 gtcggtgcat acgagcagcc gaccgtggtc gggtagtcgc agcacgcgtg ccatcgattc
     1381 gtcacgctat caggggttcc ccagggccgg tagccgcgtg gtccgacaat cgctacacgc
     1441 acaggcgagc cggctgtgcc acattttcca catcatgcgt cgtgtgagag ccaacgtgac
     1501 cggccgcgtc cagggcgtgg cgtatcgcgc gtcgaccgtc gagcaggcgc gcgtgcatgg
     1561 cgtcaccggc tgggtccgca acctgcccga cggctcggtc gagctggagg cccagggcga
     1621 cgaggcgcgg gtctccgcgc tgctcgcctg gtgccggcac ggaccgccgg ccgcgcgcgt
     1681 cgccggcgtg acggtcgagg accgcgagat cgtcgcaggc gagcgcgact tcgtacagcg
     1741 gcgctgaacg gcagcgcgtc gcagggtgtc ccgcgctcac gacgtgagcc cgagcagctc
     1801 ctgggcttcc ggatccggcg ggcggcacac gacccggtcg acggtggcca tcttggcgtg
     1861 gatcacgtcg cacaccgggg tgaacgacgc cttctggaac gcgcgcagtg acgcgtggtt
     1921 gtcggccgcg atcagcgccc acgagcggta ggcgtcggtg gcgcgcagct ccttggtgag
     1981 gtgctccagc atgagcggcg cgaccgcgcg accgcgcgcg gcgggggcga cgtacacgct
     2041 gtggatgtag gcgtcgtact tggccatccg cagctcgcgg cccagctcgg gcgcctcgat
     2101 cggcccgcgg gcggcccacg cgatgccggc cgggcgaagg ccgagcgtcg cgagcaccgc
     2161 ctggtcgccg cgccgccact gcatccgcgc ggtggcctcg tcgagctcga gctgatcgag
     2221 cagctcggcc ttgcgcgcgt cgtcgagctt gtcgtaatcg gcctccgagg acgtggcgag
     2281 ctcgaggccg gcg
//
LOCUS       DASVOR010000805         2578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387495,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000805 DASVOR010000000
VERSION     DASVOR010000805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387495"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1506)
                     /locus_tag="VF516_15950"
     CDS             complement(<1..1506)
                     /locus_tag="VF516_15950"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8109228.1"
                     /translation="MPGLSKVIVLDPDARARRQVQLGFERDGVPTAAAATAAELDGPG
                     DDTGLIVVGGIDGHGVELVRRARGWLDGRGIDAPLVFAGRGAEPRDATAAGADEVVRH
                     PAYLRDVVTIGRLLRGRPADQRGRIVGNLIELTGVFTLVRALSALGRSATLTLIRGLR
                     RGEVRFYFGEVTSAQVGLIHGQAALHQLLLWTDARFDFHHEDVVRRHQIPLSRDELFA
                     DAEQFLEGVRDSSGGLSPAMVLEQDLPRVQSLAKQIPTEVYGVLRMFDGHRALADVLE
                     DSAYRVFETLRVAQRAVEAGLIRVIDKPPPRPGWRTILALEDWLVGGEPRDAVAGSDA
                     TEAASEPVNEAGNEAGNEAVNEAMNDAGNEAVNEAMNDAGKAVNVAVNDPRPATIRDA
                     RGAPVPPARASGQGAGRSAGPGTGQGAGQGAGQGAGQGAGKTARKRRKKKRRTDTPPA
                     IAIGASGLPEINWGALVPRIVGAEVGPLAGVVPASHVSGEIVTTARTSGGND"
     gene            1567..>2578
                     /locus_tag="VF516_15955"
     CDS             1567..>2578
                     /locus_tag="VF516_15955"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8109229.1"
                     /translation="MIALEILHGTASQRLHRISGAGAAVTTIGRSPDSTVVIGDFHLS
                     GDHAQITRVGDHYVFRDLRSTNGSAIERDGRRIPVDAAARWEIALADGDVLLLGNHHD
                     PVRIAVRIGDDDGPAELGERLIASRSIRNLPAVADQLERDPGEALRVYKSLQPLGARL
                     EHGEVIEACLSAAFQLLPRATHVAILLRSETDKDRFALAVSRERGKPEPRDPRDPREP
                     RDPRTGGDAVRASRAVLRRVLADRAAVLTANAQEALSSESILGGQILSILALPLWRGD
                     DIIGLIQADNRASAGMFSETDLEVGLLLAAQASLAIDNATLVQRLRVAEERARGENVY
                     LRR"
BASE COUNT          325 a         1013 c          895 g          345 t
ORIGIN      
        1 gtcgttgccg ccggacgtcc gtgccgtcgt cacgatctcc cccgaaacgt ggcttgccgg
       61 cacgaccccg gcgagcggcc cgacctccgc accgacgatc cgcggcacca gcgcgcccca
      121 gttgatctcc gggaggcccg aggcgccgat cgcgatcgcc ggcggcgtgt cggtgcggcg
      181 cttcttcttg cgccgcttgc gcgcggtctt tccagcgccc tggccagcgc cctggccagc
      241 gccctggcca gcgccctggc cagtgcccgg accggcgctg cggccggctc cttgtcccga
      301 ggcccgggcc ggcgggaccg gtgcgccccg ggcatcgcgg atcgtggccg gccgcgggtc
      361 gttcaccgca acgttcacgg ccttcccggc gtcgttcatc gcctcgttca cggcctcgtt
      421 cccggcgtcg ttcatcgcct cgttcacggc ctcgttcccg gcctcgttcc cggcctcgtt
      481 caccggctcg ctcgccgcct cggtcgcatc gctccccgcg accgcgtcgc gcggctcgcc
      541 gccgaccagc cagtcctcga gcgccaggat cgtgcgccag cccgggcgcg gcggcggctt
      601 gtcgatcacg cggatcaggc cggcctcgac cgcgcgctgc gcgacccgca gggtctcgaa
      661 cacgcggtag gccgagtcct ccagcacatc ggccagcgcg cggtggccgt cgaacatccg
      721 cagcacgccg tagacctcgg tggggatctg cttggccagc gactggaccc gcgggaggtc
      781 ctgctccagc accatcgccg gcgacagccc gccgctggag tcgcgcacgc cctccaggaa
      841 ctgctcggcg tcggcgaaca gctcgtcgcg cgacagcggg atctggtggc gccggaccac
      901 gtcctcgtgg tggaagtcga accgcgcgtc ggtccacaac aggagctggt gcagcgcggc
      961 ctgaccgtgg atcagtccga cctgcgccga cgtgacctca ccgaagtaga acctcacctc
     1021 gccgcgccgc agcccgcgga tcagggtgag cgtcgcgctc cggcccagcg cggacagcgc
     1081 gcgcaccagc gtgaacaccc cggtgagctc gatcaggttt ccgacgatgc ggccgcgctg
     1141 atcggccggc cgcccgcgca gcagccggcc gatcgtcacg acatcgcgca gatacgccgg
     1201 gtgccgcacc acctcgtcgg ccccggccgc ggtcgcgtcg cggggttcgg cgccccggcc
     1261 ggcgaacacc agcggcgcgt cgatgccgcg gccgtcgagc cagccccggg cccggcgaac
     1321 cagctccacg ccgtggccgt cgatgccgcc gaccacgatc agccccgtgt cgtctccggg
     1381 gccgtccagc tccgccgcgg tcgcggccgc cgcggtgggg acgccatcgc gctcgaaccc
     1441 cagctggacc tgccggcggg cccgcgcatc gggatcgagc acgatgactt tcgagagtcc
     1501 cggcatgcgt cgcgagagag tgtgcagtat aaacctcgat caggagtggt gcagcgccac
     1561 gtttccgtga tcgccctcga aatcctccac ggcaccgcgt cgcaacggct gcatcggatc
     1621 tccggcgcgg gggccgccgt cacgaccatc ggccggtccc ccgatagcac ggtggtgatc
     1681 ggcgacttcc acctgtccgg cgaccacgca cagatcacgc gggtcggcga tcactacgtg
     1741 ttccgcgacc tgcggtcgac caacggctcg gcgatcgagc gcgacggccg ccggatcccg
     1801 gtcgacgccg cggcgcgctg ggagatcgcg ctggccgacg gcgacgtgct gctgctcggc
     1861 aaccaccacg acccggtgcg gatcgcggtg cggatcggcg acgacgacgg tcccgccgag
     1921 ctcggcgagc ggctgatcgc gtcgcgctcg atccgcaacc tgccggcggt cgccgaccag
     1981 ctcgagcgcg atccgggcga ggcgctccgg gtctacaagt cgctccagcc gctcggcgcc
     2041 cggctcgagc acggcgaggt gatcgaggcc tgcctgtccg ccgcgttcca gctcctgccg
     2101 cgcgccaccc acgtcgcgat cctgttgcgc tccgagaccg acaaggaccg gttcgcgctg
     2161 gcggtgtcgc gcgagcgcgg caagccggag ccgcgcgacc cgcgcgaccc gcgggagccg
     2221 cgcgacccgc gcaccggcgg cgatgccgtg cgcgccagcc gcgccgtgct gcgccgcgtg
     2281 ctcgccgatc gcgccgcggt gctgaccgcc aacgcgcagg aggcgctgtc gagcgagtcg
     2341 atcctcggcg gccagatcct gtcgatcctc gcgctgccgc tgtggcgcgg cgacgacatc
     2401 atcggcctga tccaggccga caaccgcgcg tcggccggca tgttcagcga gaccgacctc
     2461 gaggtcggcc tgctgctcgc cgcccaggcg tcgctggcga tcgacaacgc cacgctggtc
     2521 cagcggctgc gggtcgccga ggagcgcgcc cggggcgaga acgtctacct gcggcgca
//
LOCUS       DASVOR010000806         3306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_504494,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000806 DASVOR010000000
VERSION     DASVOR010000806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504494"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1431)
                     /locus_tag="VF516_15960"
     CDS             complement(205..1431)
                     /locus_tag="VF516_15960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8109230.1"
                     /translation="MNSGDTAWLLVSAALVMFMTPGLALFYGGMVQGKNVLSTLMHSF
                     FALGLVSVQFAFIGYSLCFGPTWHGVIGGLDFLGLSGVGDQAFDSSHQFPMLAHFAYQ
                     CMFAVITPALISGAYAERMKFSAYVVFTLAWTTLVYDPIAHWVWQSDGWLAQLGAIDF
                     AGGTVVHLSSGVSALICALVLGKRRGYPAVRHPPHDLTLTVIGAGILWFGWFGFNAGS
                     ALHADGLASLAFVNTHLAAAAGALAWAIIEASRAKKATMLGVASGLVAGLVAITPAAG
                     YISPMAAIGLGGAAGLICYGGVLLKTRLGYDDTLDAFGVHGIGGATGALATGLLTRAA
                     LNNGHGGGLDLLSKQAIGIAAAGAWAAVVTFVVLKGIDLVIGLRVDAETEHDGLDGAL
                     HGESAYGSPGSVAHGS"
     gene            complement(1553..2185)
                     /gene="upp"
                     /locus_tag="VF516_15965"
     CDS             complement(1553..2185)
                     /gene="upp"
                     /locus_tag="VF516_15965"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEX8109231.1"
                     /translation="MAGTVHVIDHPLVQHKLSLMRSKDTSTSTFRNLLREISMLLAYE
                     VCRDLPTTLIPIETPIAPMMAPILDGKKTVLVSIMRAGSGILDGMLEILPSARVGHIG
                     LYRDPKTLVAVEYYYKVPDNMHDRDAIVVDPMLVTGNSAVAAVDRLKESSPRSIKFVC
                     LLTCPEGLAAFHAQHPDVPVYTASIDERLNEKSYIVPGLGDAGDRLFGTK"
     gene            complement(2231..2380)
                     /locus_tag="VF516_15970"
     CDS             complement(2231..2380)
                     /locus_tag="VF516_15970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109232.1"
                     /translation="MSHLENIATRQRKSRVRDALFATFVALAAITAVTTVSQAVVASS
                     PSQHR"
     gene            complement(2788..>3306)
                     /locus_tag="VF516_15975"
     CDS             complement(2788..>3306)
                     /locus_tag="VF516_15975"
                     /inference="COORDINATES: protein motif:HMM:NF019570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1688 family protein"
                     /protein_id="HEX8109233.1"
                     /translation="LDGLAPIWPGRIELDGVNLGDVWRHPAAGGTGPSAGLVPFHKLS
                     QWLAYSLFEPLERTGRQITQPGALTGLPEYRNGGLLIDLGVLVPRRDDIRTAVHAPGD
                     EVIVEWRALTVALLDRVAEAVRQRLGKTPTELPLARVLEGGTWAAGRAVARDLRPGGG
                     PPLHIRSDGTVF"
BASE COUNT          515 a         1112 c         1207 g          472 t
ORIGIN      
        1 ggggccgcgc cgggggcggc cgggggcgca gtggccggag cgtcggggcg cgggccagcg
       61 gcgtccttgt cggcgcgtcc agtgggatcg gggactttct tcttcaccgg tgccaagctg
      121 ccgtgagtat acccagccgg ccgcggcgtg cggcggccgg gagcgggtta ctcaggtctc
      181 acggatccgc cggccggggg gcggtcagct gccgtgggcc acgctgccgg ggctgccgta
      241 cgctgattcg ccgtgcaggg cgccgtcgag gccgtcgtgc tcggtctcgg cgtcgacccg
      301 aagcccgatc accaggtcga tgcccttgag caccacgaag gtgacgaccg cggcccacgc
      361 cccggctgcc gcgatgccga tcgcctgctt cgacagcagg tcgaggccgc cgccgtggcc
      421 gttgttgagc gccgcgcggg tgagcaggcc ggtggccagc gcgccggtcg cgccgccgat
      481 gccgtgcaca ccgaacgcgt cgagcgtatc gtcgtagccc agccgggtct tgagcagcac
      541 cccgccgtag cagatgaggc cggcggcgcc gccgaggccg atcgccgcca tcggcgagat
      601 gtagccggcc gccggggtga tcgcgaccag gccggcgacc aggccggacg cgacgccgag
      661 catcgtcgcc ttcttggcgc gcgacgcctc gatgatcgcc caggccagcg cgccggccgc
      721 ggcggcgagg tgggtgttga cgaacgcgag cgacgccagg ccgtcggcgt gcagcgccga
      781 cccggcgttg aagccgaacc agccgaacca caggatgccg gcgccgatca cggtcagcgt
      841 caggtcgtgc ggcgggtggc gcaccgccgg gtagccgcgg cgcttgccga gcacgagcgc
      901 gcagatcagc gcggagacgc ccgacgacag gtgcacgacc gtgccgcccg cgaagtcgat
      961 cgcgccgagc tgggcgagcc agccgtcgga ctgccacacc cagtgggcga tcgggtcgta
     1021 gaccagcgtg gtccacgcca gcgtgaacac cacgtaggcc gagaacttca tgcgctcggc
     1081 gtaggcgccc gagatcagcg ccggggtgat caccgcgaac atgcactggt aggcgaagtg
     1141 ggcgagcatc gggaactggt gcgagctgtc gaacgcctga tcgccgacgc cgctcaggcc
     1201 caggaagtcg agcccgccga tcacgccgtg ccaggtcggc ccgaagcaca ggctgtagcc
     1261 gatgaacgcg aactgcacgc tgacgaggcc gagcgcgaag aagctgtgca tcagcgtcga
     1321 cagcacgttc ttgccctgga ccatgccgcc gtagaacagc gccagcccgg gcgtcatgaa
     1381 catcaccagt gcggccgaga cgagcagcca agcggtatcg cccgagttca tcccaccatc
     1441 ctgaccggct cgtgtttccg gcgcgtaacc ggcgcgaaaa agcttcgggc gagtgtcgcg
     1501 ttcgacgtga accgcgcttt actttcgagg agaggccccc tcgaatccgg atctacttgg
     1561 tgccgaacag gcggtcgccg gcgtcgccga ggccggggac gatgtagctc ttctcgttga
     1621 gccgctcgtc gatcgacgcg gtgtagaccg ggacgtcggg gtgctgggcg tggaacgcgg
     1681 cgaggccctc ggggcaggtc agcagacaca cgaacttgat cgatcgcggc gagctctcct
     1741 tgagccggtc gacggccgcg acggcggagt tgccggtcac gagcatcgga tcgacgacga
     1801 tcgcgtcgcg atcgtgcatg ttgtcgggga ccttgtagta gtactcgacc gcgaccagcg
     1861 tcttggggtc gcggtacagg ccgatgtggc cgacccgcgc cgacggcagg atctcgagca
     1921 tcccgtcgag gatgccgctg ccggcgcgca tgatcgagac cagcacggtc ttcttgccgt
     1981 cgaggatcgg cgccatcatc ggcgcgatcg gggtctcgat cgggatcagc gtcgtcggca
     2041 agtcccggca cacctcgtag gccaggagca tgctgatctc gcggagcagg ttgcgaaagg
     2101 tgctggtgct ggtgtccttc gatcgcatca gcgacagctt gtgttgcacc agcggatggt
     2161 cgatgacgtg gaccgtgcct gccatgtcgg cggaaaatac cgcagacacg acaggcacgt
     2221 ggcctggcgc ctagcgatgc tggctcggcg agctcgccac gacggcctgc gagaccgtgg
     2281 tgaccgccgt gatcgccgca agcgcgacga acgtggcgaa gagcgcgtcg cgaacccggc
     2341 tcttgcgctg gcgcgttgca atgttctcca gatggctcat gttcagactt cctgttggtg
     2401 cggcaggtgc cgcggtggcg cccctatatt cagcttccgc gccaacttgg accaaacgaa
     2461 ttcaagtgct tagagtgata actacggggc gcgacgtgtc gcgtcaaatg tcgccccctt
     2521 gtcgctgtcg cacccgtgtc ctacccccgc gaaaccgcgc cggatcaggc gccaggccga
     2581 gcggttgaca gtgtcgcgca tccgaatgca ccagcgcgca cggagcgatc tcctcgacgg
     2641 ccatcgctgc ccggtcacac aggttttcgt cggtgggctc gcttgcagac tccgccgcac
     2701 ccgaagcgcg tcgcgggccg gatcggtgcc agaagcgacg tcagcgccgc ccgaacggac
     2761 gcaagatgtg acgcccgacg tcggtggtca gaacaccgtt ccatcggacc ggatgtggag
     2821 cggcggccca ccgcccggcc gcaggtcgcg ggcgacggcg cgccccgcgg cccaggtgcc
     2881 gccctcgagg acgcgggcga gcggcagctc ggtgggggtc ttgccgagcc gctggcggac
     2941 cgcctcggcg acgcggtcga gcagcgcgac ggtgagcgcg cgccactcga cgatgacctc
     3001 gtcgccgggc gcgtggaccg cggtgcgaat gtcgtcgcgc cgcggcacca ggacgccgag
     3061 gtcgatcagg aggccgccgt tgcgatactc ggggagcccg gtcagcgcgc cgggctgggt
     3121 gatctgccgg ccggtgcgct ccagcggctc gaacagcgag tacgcgagcc actgcgacag
     3181 cttgtggaag ggcaccaggc cggccgatgg tccggtgccc ccggcggccg gatggcgcca
     3241 cacgtcgccg aggttgaccc cgtcgagctc gatccggccg ggccagatcg gcgcgaggcc
     3301 gtcgag
//
LOCUS       DASVOR010000807         3661 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_358557,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000807 DASVOR010000000
VERSION     DASVOR010000807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358557"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VF516_15980"
     CDS             complement(<1..1542)
                     /locus_tag="VF516_15980"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109234.1"
                     /translation="MRNLGIFTTLGLAAALSGCADHPYDPGAPAIDPTAPRVHILAPA
                     RGTFAGNVQTLTVTGTALDDTGVAAVQVNGVAAMVAGDGSWTATIPVAPGPQLIHAVA
                     RDAQGNVGKESRAVVVGPLQPIAAPVPQAIAAAMSAQTFAAIGRGITGFLSTGDLAAA
                     VAPLNPVIDVGGGPDCNYVQAAITKLSVGSASSVTLLPEPGGLALDVELDRVTLGMHL
                     AYSVVCLDGASDLTITASHIKVAGMLGVGVKAGAFDIALTGQTVDLTGVNIDVTGLPG
                     EVISWLDLDHALASVVGWAARQLVVPALNSALGSLNDTRTVNVLGTAVDIHLAPAHIA
                     FDASGAVIELDTVLRAQGDAGAPGYVFLPSQEPAMSTDRGFALAVADDAANQLLTSLW
                     AARGLDRGFDLATGSYGDIGKLYDRVELSAGVPPFIAASSGSHRLTIGDLIASFKNGD
                     ALATQIAVSAEVDVRVVADATGRPRLDVGTPTTYVDVLDEHVDGANVLSNAQFEVIAS
                     FALARV"
     gene            complement(1494..2123)
                     /locus_tag="VF516_15985"
     CDS             complement(1494..2123)
                     /locus_tag="VF516_15985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109235.1"
                     /translation="MTGGRSLVSILPLGVATALAISLAGPPAWAHVFPPLHTVVVQVE
                     RCELAVLIGYAAGTGEPTERIIARAASQPKPHALDALRASLTAFAFAPLSFTLDGRPL
                     SPTSVHAKIGVDGGGTRPMVVVLATFALPGGGELAIRSRDPRTTRISWQDRSSGRIAL
                     DRAPAQDHWYTAVASFLLPLVSPLSSPLAREGSACATSGSSPRLDSPPR"
     gene            complement(2120..>3661)
                     /locus_tag="VF516_15990"
     CDS             complement(2120..>3661)
                     /locus_tag="VF516_15990"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX8109236.1"
                     /translation="TYDLIITRGPEYEITQKTVVLRPGAFTAEQVALVRAYRSDGWIA
                     GDFHLHAQPSTDSGLPIATRVASCAAEGLEAAISTDHNYITDYAPAIAASGLDGWLLG
                     IPGMELTTFEMGHFNGYPLRVDPGSTRGGEFRWTGTPPQGLFDQLRALAIDSGSGTGR
                     GIVQVNHPRQAVLGYFAQFFVDQATGQPYTPTGILGIFAPYGDEFQHENYSTDFDAIE
                     ILTGSRLEDVHTFRAPDPLPPGPFPDPQPVPGQIVVGPDGRPSFPGVVETWFTLLDRG
                     HRATAMGTSDSHHLLGDEPGYARTLLHVGAGKDVPGGYTRDDVIDAVRGHRAITTNAP
                     FVELTIGDARIGDTITAPGGSVDVDVHVRSPSWARVDHLVVYANSQIVADQAIPASQG
                     TDYQTRIHLRLAVDSWVVAEATGKGNLFPVVSPVEFPPLDATVLIRALSHGLDLGALP
                     LTSNLRPSSTHIVTPYAITNPIWIDVDGNGWTPPRPPLPRRAAPGGPPPDIRARFDAL
                     PEISR"
BASE COUNT          523 a         1193 c         1453 g          492 t
ORIGIN      
        1 cacgcgcgcc agcgcgaacg aggcgatgac ctcgaactgg gcgttggaca gcacgttggc
       61 gccgtcgacg tgctcgtcga gcacgtcgac gtaggtggtc ggcgtgccga cgtcgagccg
      121 cggccggccg gtggcgtcgg caaccacccg gacgtcaacc tcggcgctga ccgcgatctg
      181 ggtggcgagc gcgtcgccgt tcttgaagct ggcgatcagg tcgccgatcg tgagccggtg
      241 cgaaccgctg gatgccgcga tgaacggcgg gacgccggcc gacagctcga cgcgatcgta
      301 gagcttgccg atgtcgccgt agctgccggt ggcgagatcg aagccgcggt cgaggcccct
      361 ggcggcccac aggctggtga gcagctggtt ggcggcgtca tcggcgaccg ccagggcgaa
      421 gccgcgatcg gtggacatcg ccggctcctg gctgggcagg aacacgtagc ccggcgcgcc
      481 ggcgtcgccc tgcgcgcgca gcacggtgtc gagctcgatc accgcgccgc tggcgtcgaa
      541 cgcgatgtgc gccggggcga ggtggatgtc gacggccgtg ccgagcacgt tgaccgtccg
      601 ggtgtcgttg agcgagccga gcgcgctgtt gagcgccggc acgacgagct ggcgcgcggc
      661 ccagccgacg accgaggcga gcgcgtggtc gaggtcgagc cagctgatga cctcgcccgg
      721 caggccggtg acgtcgatgt tgacgccggt gagatcgacg gtctggccgg tcagtgcgat
      781 gtcgaacgcg ccggccttga cgccgacgcc gagcatgccg gcgaccttga tgtggctcgc
      841 ggtgatcgtg agatcgctcg cgccgtcgag gcacaccacc gagtacgcca ggtgcatgcc
      901 gagcgtgacg cgatcgagct cgacgtcgag cgcgaggccg ccgggctcgg ggagcagggt
      961 gacgctgctc gcgctgccga ccgacagctt cgtgatcgcg gcctggacgt agttgcagtc
     1021 ggggccgccg ccgacgtcga tcaccgggtt gagcggcgcg accgcggccg ccaggtcgcc
     1081 ggtcgaaagg aagccggtga tgccgcggcc gatcgcggcg aaggtctgcg ccgacatcgc
     1141 ggcggcgatc gcctggggga ccggggcggc gatcggctgg agcgggccga cgacgaccgc
     1201 gcgcgactcc ttgccgacgt tgccctgtgc atcgcgcgcc accgcgtgga tcagctgggg
     1261 gcccggcgcg accgggatcg tcgcggtcca cgagccatcg ccggcgacca tcgcggcgac
     1321 gccgttgacc tgcaccgccg cgacgccggt gtcgtcgagc gcggtgccgg tgacggtcag
     1381 ggtctggacg ttgccggcga acgtgccgcg cgcgggcgcg aggatgtgca cgcgaggcgc
     1441 ggtcggatcg atcgccggag cgccggggtc gtagggatgg tcggcgcagc cgctcagcgc
     1501 ggcggcgagt ccaagcgtgg tgaagatccc gaggttgcgc atgccgagcc ttccctggcc
     1561 aggggcgacg aaagaggcga caccagaggc agcaagaagg aggccacggc cgtgtaccag
     1621 tggtcctggg cgggagcgcg atcgagggcg atccggccgc tcgagcgatc ctgccaggat
     1681 atccgcgtgg tgcgcgggtc ccgcgagcgg atcgcaagct cgccaccgcc gggcagcgca
     1741 aacgtcgcca gcacgaccac catcggccgc gtcccaccgc catcgacgcc gatcttggcg
     1801 tggacggagg tgggcgagag cggccggccg tcgagcgtga acgacagcgg cgcaaaggca
     1861 aacgcggtga gcgaggcacg cagtgcgtcg agcgcgtgtg gtttcggctg gctggcggcg
     1921 cgggcgatga tccgctcggt cggctcgccg gtgcctgccg cgtagccgat cagcaccgca
     1981 agctcacagc gctcgacctg gaccaccacc gtgtgcagcg gcgggaacac atgcgcccag
     2041 gctggcggtc cagccagcga gatggccagt gcagtagcca ccccgagcgg caggatcgac
     2101 acaagggagc gcccgccggt catcgcgaga tctccgggag cgcgtcgaac cgggcacgga
     2161 tgtcgggcgg cggcccaccg ggcgccgcgc ggcgcggtag cggcggccgg ggcggagtcc
     2221 agccgttgcc gtccacgtcg atccagatcg gattggtgat cgcatagggc gtcacgatgt
     2281 gggtcgagct cggccggagg ttggaggtca gcggcagcgc gccgagatcg agcccgtgcg
     2341 acagcgcccg gatcaggacg gtggcgtcga gcggcgggaa ctcgaccggc gacaccaccg
     2401 gaaacaggtt gcccttgccg gtggcctcgg cgacgaccca gctgtcgacc gcgaggcgga
     2461 ggtggatgcg ggtctggtag tcggtgccct ggctggccgg gatcgcctga tcggcgacga
     2521 tctggctgtt ggcgtacacg accaggtggt cgacccgggc ccacgacggc gagcgcacgt
     2581 ggacgtcgac gtcgacgctg ccgccgggcg cggtgatcgt gtcgccgatc cgggcgtcgc
     2641 cgatcgtcag ctcgacgaac ggcgcgttgg tggtgatcgc gcggtggccg cggaccgcgt
     2701 cgatcacgtc gtcgcgggtg tagccgcccg gcacgtcctt gccggcgccg acgtggagca
     2761 gggtgcgcgc gtagcccggc tcgtcgccga gcaggtggtg gctgtcggag gtgcccatgg
     2821 cggtggcccg gtggccgcga tcgagcaggg tgaaccaggt ctcgaccacg ccggggaacg
     2881 acgggcggcc gtcgggtccg acgacgatct ggccgggcac cggctggggg tccgggaacg
     2941 gccccggcgg cagcggatcg ggcgcgcgaa acgtgtggac gtcctcgagc cggctgccgg
     3001 tgaggatctc gatcgcgtcg aagtcggtcg agtagttctc gtgctggaac tcgtcgccgt
     3061 agggcgcgaa gatcccgagg atgccggtcg gcgtgtacgg ctggcccgtc gcctggtcga
     3121 cgaagaactg cgcgaagtag ccgagcacgg cctggcgcgg gtggttgacc tggacgatgc
     3181 cgcgcccggt gccggagccg gaatcgatcg cgagcgcgcg cagctgatcg aacagcccct
     3241 ggggcggcgt gccggtccag cggaactcgc cgccgcgtgt cgagcccggg tcgacccgga
     3301 gcgggtaccc gttgaagtgg cccatctcga acgtggtgag ctccatcccg gggatcccga
     3361 gcagccagcc gtcgagcccg ctcgcggcga tcgccggcgc gtagtcggtg atgtagttgt
     3421 gatcggtcga gatcgcggcc tccaggccct cggcggcgca gctcgcgacc cgggtggcga
     3481 tcggcagccc gctgtcggtc gacggctgcg cgtggaggtg gaagtcgccc gcgatccagc
     3541 cgtcggagcg gtaggcgcgg accagcgcga cctgctcggc ggtgaacgcg ccggggcgca
     3601 gcacgacggt cttctgcgtg atctcgtact cgggcccgcg cgtgatgatc aggtcgtagg
     3661 t
//
LOCUS       DASVOR010000808         2448 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_416437,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000808 DASVOR010000000
VERSION     DASVOR010000808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416437"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1286
                     /locus_tag="VF516_15995"
     CDS             <1..1286
                     /locus_tag="VF516_15995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109237.1"
                     /translation="SPASAAAEADDAGDPTGTDADPAALAAKVQDLEDKLASLQNQQA
                     NKPFPIKITGYGDLGAFATQGDGTGFRRDIGHSAMPSLANYGWVFYGDLLATQINSRG
                     DAADLGQAPGVDRFDSVHSGGHLTFLVNELNLGVKAGLGPTAMFTGSVNFTPRSGSNF
                     SLGDAFDIDIAQLEWMPTDDGKTSIFIGKVDSVLGNEYKTRRASDRFGITPSLMARYT
                     TGTAIGIKARTLLLDDHLILAAAVTNGSFVTEQFFFSQETDSNNFKTVSGRAALRFPI
                     LGGTVEVGPSGSWGTQDTTPDNGPKMWFVGVDAELAMPRIAVKAQWLKGKAPGDPDTM
                     TYALDLKQGGYVEADVLVTPLIGVLGRAEFRDADMTLGQERMYITRNWRATGGVRLSI
                     SPNATLKAEYSHNGEYGGTPSIPDDVVTTSAVMAF"
     gene            1297..2274
                     /locus_tag="VF516_16000"
     CDS             1297..2274
                     /locus_tag="VF516_16000"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEX8109238.1"
                     /translation="MRTPQGSRLALLTLASGLGFTLAPSAQAQPRRPAPTPAVTKADV
                     ERLDRKIEEQQQRLDKLIKLQIQYLQLLAAMTDGHTPPPVVTPEPKPDTPPKPEPKTE
                     PKPEPKPVVAAAAEPKPKAAPPPGPVPAAAKPKPKAEAVGNVVGKVTGAADAVVYVDD
                     IVVPTRGTATMKQEGKQFMPPVLIVQKGTTVQFPNLDAFFHNVFSVTPDNSFDLGSYR
                     QGETKGVTMSKPGVVNVYCNMHPQMVGHILVVPNGNYVHAGKDGFFRLSNVPTGHHRI
                     VAWAPDSKPVVTEADVNETEAATVELELKRGRTGPHLKKDGLPYGSYDK"
BASE COUNT          420 a          946 c          775 g          307 t
ORIGIN      
        1 cctcgccggc gagcgccgcc gccgaggccg acgatgccgg cgacccgacc ggcaccgacg
       61 ccgaccccgc ggcgctcgcc gccaaggtcc aggacctgga ggacaagctg gcgtcgctgc
      121 agaaccagca ggccaacaag ccgttcccga tcaagatcac cggctacggc gatctcggcg
      181 cgttcgccac ccagggcgac ggaaccggct tccgccgcga catcggccac tcggcgatgc
      241 catcgcttgc caactacggc tgggtgttct acggcgacct gctcgcgacc cagatcaact
      301 cgcgcggcga cgccgccgac ctcgggcagg ccccgggcgt cgatcgcttc gactcggtgc
      361 actcgggcgg ccacctgacg ttcctggtca acgagctgaa cctcggcgtc aaggccggcc
      421 tcggcccgac cgccatgttc accggcagcg tcaacttcac cccgcgctcc ggcagcaact
      481 tcagcctcgg cgacgcgttc gacatcgaca tcgcgcagct cgagtggatg ccgaccgacg
      541 acggcaagac gtcgatcttc atcggcaagg tcgactcggt gctcggcaac gagtacaaga
      601 cccgcagggc gtccgatcgg ttcggcatca cgccgagcct gatggcgcgc tacacgaccg
      661 gcaccgcgat cggcatcaag gcgcgcacgc tgctgctcga cgaccacctg atcctcgccg
      721 cggcggtcac caacggctcg ttcgtcaccg agcagttctt cttctcccag gagaccgact
      781 ccaacaactt caagaccgtg tcgggccgcg ccgcgctccg gtttccgatc ctcggcggca
      841 cggtggaggt cggcccgtcg ggctcgtggg ggacccagga caccacgccc gacaacggcc
      901 ccaagatgtg gttcgtcggc gtcgacgccg agctcgccat gccgcgcatc gccgtcaagg
      961 cgcagtggct caagggcaag gcgccgggcg acccggacac catgacctac gccctcgatc
     1021 tcaagcaggg cggctacgtc gaggccgacg tgctcgtcac cccgctgatc ggcgtgctcg
     1081 gccgtgccga gttccgcgac gccgacatga ccctcggcca ggagcggatg tacatcaccc
     1141 ggaactggcg ggcgaccggc ggcgtccggc tgagcatcag cccgaacgcg accctcaagg
     1201 ccgagtactc gcacaacggc gagtacggcg gcacgcccag catccccgat gacgtcgtca
     1261 ccacgtcggc cgtgatggcc ttctgaaagg agtcccgtgc gcacacccca aggctcccgc
     1321 ctcgcgctgc tcaccctcgc cagcggcctc ggcttcacgc tcgcgccatc ggcgcaggcc
     1381 cagccgcgcc ggcccgctcc caccccggcc gtcaccaagg ccgacgtcga gcggctcgac
     1441 aggaagatcg aggagcagca gcagcggctc gacaagctga tcaagctgca gatccagtac
     1501 ctccagctgc tcgccgcgat gaccgacggc cacacgccgc cgcccgtggt cacgcccgag
     1561 cccaagcccg acaccccgcc caagcccgag cccaagaccg agcccaagcc cgagcccaag
     1621 ccggtggtcg ccgccgccgc cgagcccaag cccaaggccg cgccgccacc ggggcctgtg
     1681 cccgcggcag ccaagcccaa gccgaaggcc gaggccgtcg gcaacgtggt cggcaaggtc
     1741 accggcgcgg ccgatgcggt ggtctacgtc gacgacatcg tggtgccgac ccgcggcacg
     1801 gcgacgatga agcaggaagg caagcagttc atgccgccgg tgctgatcgt ccagaaggga
     1861 accacggtgc agttcccgaa cctcgacgcg ttcttccaca acgtgttctc ggtgacgccc
     1921 gacaactcgt tcgacctcgg cagctaccgc cagggcgaga ccaagggcgt gacgatgtcc
     1981 aagccgggcg tggtgaacgt gtactgcaac atgcacccgc agatggtcgg ccacatcctg
     2041 gtcgtcccca acggcaacta cgtgcacgcc ggcaaggacg ggttcttccg gctgtcgaac
     2101 gtgccgaccg gccaccatcg catcgtcgcc tgggcgcccg actccaagcc ggtcgtcacc
     2161 gaggccgacg tcaacgagac cgaggcggcg acggtcgagc tcgagctcaa gagggggcgc
     2221 accggcccgc acctcaagaa ggacgggctg ccctacggca gctacgacaa gtgatcatgg
     2281 tcgcgccgcg cgccatcggc tcccgctcgc gcatcccgcg cggagtgtgc cgtgcgcgcg
     2341 cgcgcgacgc ctcgaacggc tcgatcccac ggggggcgct gccccccgcc ccgccggcga
     2401 agccgtcggg gtccccaccc cccgcctcgc ttcgctcgat cccacggg
//
LOCUS       DASVOR010000809         2080 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_212550,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000809 DASVOR010000000
VERSION     DASVOR010000809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212550"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2080)
                     /locus_tag="VF516_16005"
     CDS             complement(<1..>2080)
                     /locus_tag="VF516_16005"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HEX8109239.1"
                     /translation="GMARRIGDPALAARLGEAMRGEVLFDAFTRGRYSTDASIYQIEP
                     LGVALPRDADDVVAAIALARADGFPIIARGGGSSQNGQPLGEAVVLDTSRHMNRVLEV
                     DAEAGTATVQPGIVLDELNRQLRPTGWCFPVDVSTSAHATLGGMTGNNSAGTRSIQYG
                     LMVHNVLEVEAVLADATRVVFGELPPPGGAPARLAELAEALHTLYAANADEIARRTPK
                     VLRKVGGYNLYALGEPRPNLARLMVGSEGTLGLFTRIKLRLARIPRHRVGAVCHFGRF
                     FDAMDMTRFLVELAPSAVELVDRKMMELARALPVFRATLERHVRGAPDAILLVEFAGD
                     EQAPLIDALGRLEQLLADHGYPGSVVRLVDAAAQAEMTAVRKAGLNIMMSMKGDGKPV
                     SFIEDCAVPLPHLAEFTDRLTRLFTRHGTEGTWYAHASVGCLHVRPILNMKDPSGART
                     MRAIAEEAFSIVREYEGSHSGEHGDGLSRSEFHAMMFGDRMVRAFEAVKDAFDPRGLL
                     NPGKIVRAPRMDDRRLFRYKPGYGALPVVTALDWSDAGGLVAATEMCNNNGSCRKFDA
                     VMCPSYQATGDERDATRGRANSLRLALTGQLGPDALVSDAMKETMDLCVGCKACRREC
                     PTGVDMARMKIEFLHHWNARHGVPAQAALTAYLPRYAPVARRLAPMINLRNRVPGLAA
                     LGERLLGLSRK"
BASE COUNT          281 a          756 c          757 g          286 t
ORIGIN      
        1 cttgcgggac aggccgagca gccgctcgcc gagcgccgcc aggccgggca cgcggttgcg
       61 caggttgatc atcggcgcga gccggcgcgc gacgggcgcg tagcgcggca ggtaggcggt
      121 gagcgcggcc tgcgccggca cgccgtggcg cgcgttccag tggtgcagga actcgatctt
      181 catgcgcgcc atgtcgacgc cggtcgggca ctcgcgccgg cacgccttgc agccgacgca
      241 cagatccatg gtctccttca tcgcgtcgga caccagcgcg tcggggccga gctggccggt
      301 cagcgccagc cgcaggctgt tggcgcggcc gcgcgtcgcg tcgcgctcgt cgccggtggc
      361 ctggtagctc gggcacatca ccgcgtcgaa cttgcggcag ctgccgttgt tgttgcacat
      421 ctcggtggcg gcgacgaggc cgcccgcgtc cgaccagtcg agcgcggtga cgaccggcag
      481 cgcgccgtag cccggcttgt agcggaacag ccggcggtcg tccatccgcg gcgcgcgcac
      541 gatcttgccc gggttgagca ggccgcgcgg atcgaacgcg tccttgaccg cctcgaacgc
      601 gcggaccatg cggtcgccga acatcatggc gtggaactcc gagcggctca ggccatcgcc
      661 gtgctcaccg gagtgcgagc cctcgtactc gcggacgatc gagaacgcct cctcggcgat
      721 cgcccgcatc gtccgggcac cggacggatc tttcatgttg aggatcggcc ggacgtgcag
      781 gcagccgacc gaggcgtgcg cgtaccaggt gccctcggtg ccgtgccggg tgaacagccg
      841 ggtcagccgg tcggtgaact ccgcgaggtg gggcagcggc accgcgcagt cctcgatgaa
      901 cgacaccggc ttgccgtcgc ccttcatcga catcatgatg ttgagcccgg ccttgcgcac
      961 cgcggtcatc tcggcctgcg ccgcggcgtc gaccagccgg accaccgagc cggggtagcc
     1021 gtggtcggcg agcagctgct cgagccggcc gagcgcgtcg atcagcggtg cttgctcgtc
     1081 gcccgcgaac tcgaccagga ggatcgcatc cggcgcgccc cggacatggc gctcgagcgt
     1141 ggcgcggaac accggcagcg cgcgcgcgag ctccatcatc ttgcgatcga ccagctcgac
     1201 cgcgctgggc gcaagctcga ccaggaaccg ggtcatgtcc atcgcgtcga agaaccggcc
     1261 gaagtggcac accgcgccga cccggtggcg cgggatccgg gcgagcctca gcttgatccg
     1321 ggtgaacaac ccgagcgtgc cctcggagcc gaccatcagc cgcgcgaggt tgggccgcgg
     1381 ctcgccgagc gcgtacaggt tgtagccgcc gaccttgcgc agcaccttcg gcgtgcgccg
     1441 cgcgatctcg tcggcgttgg ccgcgtacag cgtgtgcagc gcctcggcga gctcggcgag
     1501 ccgcgccggc gcgccgccgg ggggaggcag ctcgccgaac acgacccggg tcgcgtcggc
     1561 cagcaccgcc tcgacctcga gcacgttgtg caccatcagg ccgtactgga tcgaccgcgt
     1621 gccggccgag ttgttgccgg tcatcccgcc gagcgtggcg tgcgccgagg tcgacacgtc
     1681 gaccgggaag caccagccgg tcggccggag ctggcggttg agctcgtcga gcacgatccc
     1741 cggctgcacg gtggcggtgc cggcctcggc gtcgacctcc agcacgcggt tcatgtggcg
     1801 gctggtgtcg agcaccaccg cctcgccgag cggctggccg ttctgcgagc tgccgccgcc
     1861 gcgcgcgatg atcgggaagc cgtccgcgcg cgccagcgcg atcgcggcga ccacgtcgtc
     1921 ggcgtcgcgc ggcagcgcca ccccgagcgg ctcgatctgg tagatcgacg cgtcggtcga
     1981 gtaccggccg cgggtgaacg cgtcgaacag cacctcgccg cgcatcgcct cgcccagccg
     2041 ggccgcgagc gccggatctc cgatccgccg ggccatgcct
//
LOCUS       DASVOR010000810         2762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_8269,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000810 DASVOR010000000
VERSION     DASVOR010000810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8269"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..474
                     /locus_tag="VF516_16010"
     CDS             202..474
                     /locus_tag="VF516_16010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109240.1"
                     /translation="MSDAWTVSEPMTWSEICERFPDQWIALVALDWADDHDEPFRTAL
                     VAGCGSRREAMAQARPLARLFEPIGPFFTGDPPAAQRALPPIVYVA"
     gene            complement(496..1161)
                     /locus_tag="VF516_16015"
     CDS             complement(496..1161)
                     /locus_tag="VF516_16015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109241.1"
                     /translation="MTVPRIAMVMVLGAWAGCIDSSFGGVKQANVPASQLAIEPTLVD
                     SDAAPPDGKIPVIVQFFQGNEYVMLSSATLTIDGVAVPYGSMGYTTRIPIVPSGDSIT
                     FTHVRAGVTTQFTYRVPSRPTITSPMANEVVPRSPNLMIGYASSNGLAVRPLAADAVA
                     GTSGIEQSDNGMAFLDVSGLRSGAGSVGVARRYVTTPSQTGFRSATVTYTITSLPTPV
                     TWQ"
     gene            1500..1970
                     /locus_tag="VF516_16020"
     CDS             1500..1970
                     /locus_tag="VF516_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109242.1"
                     /translation="MEQGSHGGTEIHPEDSCDPATFGALCNPSFRGTTTLAQFSAELD
                     ATRRVAAWEYGGGSGNIRLGQSLRVDNRGGEIHTFTVVAQFGGGRVPILNQKSGNTTV
                     APECVAGASATNVDIASGTGINVTTGASGAIKTRGTFKVQCCIHPWMRTTVTVQ"
     gene            complement(2105..>2762)
                     /locus_tag="VF516_16025"
     CDS             complement(2105..>2762)
                     /locus_tag="VF516_16025"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8109243.1"
                     /translation="PIASAMKLTLSFDNGPDPDVTPAVLDVLRGRGVTAQFFVLGKQL
                     GDPRCRRLVERARDAGHLVGNHSFSHAVPLGRDDRPGAVDAEIAATEALLAPLVPGPK
                     RFRPIGGGELGRHLLTRRAVDYLVAHGYSCILWNSVPRDWVDPDGWSARALADGTTRP
                     HTLLVLHDIPNACLAGLPGFIDAARAAGFELVADAPPDCAPIVDGRVVGSLDELVAAA
                     "
BASE COUNT          437 a          987 c          958 g          380 t
ORIGIN      
        1 gccgagctgg cgcgcgagcg gatcgcgatc gacgccgagc gcgcccggct cgccgtgctc
       61 gcgatcgcgc gcgacaccgc gcgcagcgac gaacgcgagc tggtcaccgc gctcgacgcg
      121 ctcaccgccg acctgccgtg acccggaccc cggccggctg aaaacttgcc cgcgccgccg
      181 aacctccaca ggctgggcac catgagcgat gcgtggacgg tctccgagcc gatgacctgg
      241 agcgagatct gcgagcgctt tcccgaccag tggatcgcgc tggtcgcgct cgactgggcc
      301 gatgatcacg acgaaccgtt ccgcaccgcg ctggtcgccg gctgcggctc gcgccgcgag
      361 gcgatggccc aggcccgccc gctggcccgg ctgttcgagc cgatcggccc gttcttcacc
      421 ggcgatccgc cggccgcgca gcgggcgctg ccgccgatcg tgtatgtcgc gtgacggcgc
      481 tcgacccgac cgcgcctact gccaggtcac cggggtcggc aacgacgtga tcgtgtacgt
      541 gaccgtcgcg gaccggaagc cggtttggct tggcgtggtg acgtaccgcc gggccacgcc
      601 gaccgacccg gctcccgacc gcaacccgga cacgtcgagg aacgccatcc cgttgtcgga
      661 ctgctcgatg ccgctggtgc cggccacggc gtccgccgcg agcgggcgca ccgccagacc
      721 gttcgaggac gcgtagccga tcatcaggtt cggcgagcgc ggcaccacct cgttggccat
      781 cggcgacgtg atcgtgggcc gcgacggcac ccggtaggtg aactgagtgg tcacccccgc
      841 ccggacatgc gtgaacgtga tcgagtcgcc cgacggaacg atcgggatcc gcgtggtgta
      901 ccccatcgag ccgtacggca ccgccacgcc gtcgatggtc agggtggccg agctgagcat
      961 gacgtactcg ttcccctgaa agaactgcac gatcaccggg atcttgccgt cgggcggcgc
     1021 cgcgtcggaa tcgacgagcg tcggctcgat cgcgagctgg ctggcgggga cgttggcctg
     1081 cttgactccg ccgaagctgg aatcgatgca cccggcccag gcacccagaa ccatgaccat
     1141 cgcgatccgc ggcaccgtca tggtcaccta ccctacttcg tttcgcgcac catgccagag
     1201 cgctcgcatc acccaggggc gccaggatta catttcttac ctattaatct gtaattttaa
     1261 ttactataat gagtcgtaat ctagcgattc ctgcaccgct ctgcgggata cccaggttga
     1321 cggaaatctg agggggcgat atggccggag gcgcaaagtt ccccgattcc tccggggaaa
     1381 aaaggagtac cggtatgtct cacttcgcct cacgcgcttc caccctctgt gcaacgagcg
     1441 cactcctgtt cgcgttcgga tgcgccgcag atccggcgga tggccccccg gagctcgccg
     1501 tggagcaagg ctcccacggg ggcaccgaga tccatcccga ggacagctgc gatccggcga
     1561 cgttcggcgc gctgtgcaac ccgagcttcc gcggcacgac cacgctcgcg cagttcagcg
     1621 ccgagctcga cgccacccgg cgggtcgcgg catgggagta cggcggcggc tcggggaaca
     1681 tccgcctcgg ccagagcctg cgggtcgata accggggcgg cgagatccac acgttcacgg
     1741 tggtggccca gttcggcggc ggccgcgtgc cgatcctcaa ccagaagtcc ggcaacacca
     1801 cggtcgcccc ggagtgcgtc gcgggagcca gcgcgaccaa cgtcgacatc gcctcgggca
     1861 ccggcatcaa cgtgaccacc ggcgccagcg gcgcgatcaa gaccaggggc acgttcaagg
     1921 tccagtgctg catccacccg tggatgcgga ccaccgtcac ggtccagtga gcgatcgacg
     1981 cggccgcgcg accggcgcgg ctgcatccag gtcgaccggc gccacgcgga cgtgaccgat
     2041 cgatcgccga cgacggcacc aacctcggcg gcggcgcggt cgacgcggcg aactgaacga
     2101 cctgctacgc cgccgcgacg agctcgtcga ggctgccgac gacgcggccg tcgacgatcg
     2161 gcgcacagtc cggcggcgcg tcggccacca gctcgaaccc ggccgcgcgg gcggcgtcga
     2221 tgaagccggg cagcccggcc aggcaggcgt tggggatgtc gtgcagcacg agcagcgtgt
     2281 gcggccgggt cgtgccgtcg gcgagcgcgc gggccgacca gccgtccggg tcgacccagt
     2341 cgcgcggcac gctgttccac aggatgcacg agtagccgtg cgccaccagg tagtcgaccg
     2401 cgcgccgggt caagaggtgg cggccgagct cgccgccgcc gatcggacgg aaccgcttcg
     2461 ggccgggcac gagcggtgcg agcagcgcct cggtcgcggc gatctcggcg tccaccgcgc
     2521 cggggcgatc gtcccggccg agcgggacgg cgtggctgaa cgagtggttg ccgaccaggt
     2581 ggccggcgtc gcgcgcgcgc tcgaccagcc ggcggcaccg cggatcgccg agctgcttgc
     2641 cgagcacgaa gaactgcgcg gtgacgccgc ggccccgcag cacgtcgagc accgccggcg
     2701 tcacgtcggg atcgggcccg ttgtcgaacg acagcgtcag cttcatcgcg ctagcgatag
     2761 gt
//
LOCUS       DASVOR010000811         9592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_96010,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000811 DASVOR010000000
VERSION     DASVOR010000811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9592
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96010"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..3049)
                     /locus_tag="VF516_16030"
     CDS             complement(155..3049)
                     /locus_tag="VF516_16030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109244.1"
                     /translation="MKAAALALVPALALLALTAPARAGDPTRVWRTIESDHFVIHYYA
                     PLDDVARRLAVVAERAHRTLSPALAHRPDSKTIVVLVDDTDSANGFASVLPRNMIQVF
                     ATGPTGFNELDDHEDWLYGLIAHEYTHILHLDTMEGLPNIYNRIFGKIWAPNQIMPRW
                     IIEGLAVYEESKRSAGGRDRGTRFDQFIRTGYDEHKDLRLDEITGAPRAFPHGNAIYV
                     YGSHFLRYIFDRFGDDVPGKMAHEGGSYAPPYAVNRQIARVVGVPFTELYDSWKRYLG
                     DRYSLQETAAERRGLAVGRALSHSAESNFWPHYSADGKEIYWVQYDGYSLPCVRAMPV
                     GGDQRQARDVARIDAMGPFDLLGDGSLVFEQTRTYRREYSYQDLMRWDGVTHQMVRLS
                     TGRRARDPAVSRDGRRVAFSMNEHSESVLAVQDLVPDAPVTVVWRGERFDQAYQPAWS
                     PDGTRIAFSAWRKGGLRDILVVELATGRVEAITDDRAIDMEPMWSRDGRYLLFDSDRT
                     GIQNIYAFDTADRSLWQVTNVLGGAFQPAPSPDGTRLAFASAVPAGGWDLYEIPLDPA
                     SWQPARDYVDDKPPAVVIRDREAAVTAPRPYRALESLAPQTWSATYNLGSVSSASVTT
                     GGSDAAGLHVYSLAVAADTDKQTPNIGAAYAYSGLRPGLRIAGARSLNDRGGFRIDGR
                     NFLFTEEDWSGALSVSIPFESRPESSWALSFNYDLDWFRLVKAPMFVLDPTQRVPTHP
                     PTDYVQSGFSSRVAYSSVKATTFGYGPTAGWDGAVSLRLDHPALGATYRNFTVAYGLD
                     IYQRLWGATPVFALRLAGAVRAGDLIGPGGFSLGGVPSQDVVMSIVNNLRFGVSGYLR
                     GYPPRVLAGNQYHLLNLEYRQELFQIERGVDTFPIYLRRVDLAVLGDGGTAFDTAFNA
                     ARDVRGSLGGALRLDAFFWYFMPGTFEIGYARGLAHDGINETWFLLTGSL"
     gene            complement(3046..4824)
                     /gene="ptsP"
                     /locus_tag="VF516_16035"
     CDS             complement(3046..4824)
                     /gene="ptsP"
                     /locus_tag="VF516_16035"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEX8109245.1"
                     /translation="MSESRHEVRRQGLGVSPGIAFGRAYLIGRDTLKAPRHHIEPDDV
                     DTEIARLYKAIAASDKQLARIKERLASENESDYHIITAHQMMLHDEHLVGAAVGYIRD
                     ELINAEWGLRRAVDDIAGVFDRMQDEYLRGRRADVEFVFERVLRSLLGRETGPVQPPP
                     DAIVVAYDLSPADTAQLHKAAVAGMITDAGGKTSHTAIIARAHEIPAVVGLENITELV
                     ETDDRLLIDGGSGIVIVNPTPSTVAEFRDEQRRQVAVSATLHALRDLPGVTLDGAEIE
                     LMCNIDGPDEIEDALDYGAMGVGLFRTEYLFMNRDDPPDEERHYQTAVDVLRRLGGKP
                     ATIRTYDLGADKLAPFLEEAELLEVNPALGLRSIRLCLSQLGRPMFKAQLRGLLRASA
                     HGPLQIMFPMISGVAELRAAKAVVDEVKLELQQEGQAFDEHVKLGIMIEMPSAALSAE
                     FLAEECDFFSIGTNDLIQYTMAVDRVNEYVSYLYEPLHPSLLRLLGQVARAAHSTGIP
                     VAVCGEMAGEPMIAPVLIGLGIRELSMSAVSVPEVKALIRATRTPETEALVQRIARLG
                     TAAEVRAMVADYVFGLTPHPGAKGPV"
     gene            complement(4832..5119)
                     /locus_tag="VF516_16040"
     CDS             complement(4832..5119)
                     /locus_tag="VF516_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEX8109246.1"
                     /translation="MAADDRAERTLQIQNELGLHARAGTKFVQAASKFTGCDITVSKG
                     NNEVNGKSILSLLTLMAPKGTTITIRARGDRAEEAVATLAALIEDKFGEGK"
     gene            complement(5119..5955)
                     /gene="rapZ"
                     /locus_tag="VF516_16045"
     CDS             complement(5119..5955)
                     /gene="rapZ"
                     /locus_tag="VF516_16045"
                     /inference="COORDINATES: protein motif:HMM:NF015620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEX8109247.1"
                     /translation="MQIVIVTGLSGAGKSTALRALEDIEFYCVDNIPVPLVAQMVEHL
                     GNEGDRDKLAIAIDSRQRRNLTAWTTVVTRLRGSGHRLEVMYLDASDEVLLRRFSETR
                     RRHPLSGDELSEGIRLDREVMADLRVGAAVIDTSYLNMHQLKAIIQERYGGTGKLAVT
                     LVSFGFKHGLPQELNHVFDVRFLPNPYFEPTLAHLDGREPQVAAYVLGPDGLELVRQI
                     EGILRFSLPQFHKEGKLYVTICVGCTGGRHRSVVIVEELRRRLGTAWDILVRHRDIDR
                     GG"
     gene            complement(5956..6915)
                     /gene="hprK"
                     /locus_tag="VF516_16050"
     CDS             complement(5956..6915)
                     /gene="hprK"
                     /locus_tag="VF516_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEX8109248.1"
                     /translation="MSSAITVGELLERAELEVTAVAGQPGLARIVTVPRIQKPGLALT
                     GWHEQLHPHRVLVLGGTEIEYLADHPPARELGIATLLASDPACVVVCRGLTPPVELVN
                     AAEQRGVPVLVSQLATADFISAVTAWMSDRLAPSTDMHGVLMDVLGIGVLLLGKSGIG
                     KSETALDLVVRGHRLVADDVIKIRRQGGHVVGRGAGIIGHHMEIRGLGIINVKDLFGI
                     SAVRDTKKIELVVELREWSEHEEYDRLGFDDRFDRILEVSVPAVQLPVRPGRNLATLI
                     EVAARNLLLKLQGTHSARAFRDQLHRAMEESEATGDVPMDAVE"
     gene            complement(6916..7377)
                     /locus_tag="VF516_16055"
     CDS             complement(6916..7377)
                     /locus_tag="VF516_16055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX8109249.1"
                     /translation="MKIVDLIRRDMVVPALQATDKRGILEELAHYMAGRHPRIDRTTL
                     SRVLIEREQLASTAIGEGVAIPHGKLGAVGEIVACLGRAPTGVDFDSMDGQPTYLFFV
                     LVAPESSTGAHLKALARISRVFKDPEFRRRLLAAPDAEAMYHVVAEEDAKY"
     gene            complement(7465..8106)
                     /gene="raiA"
                     /locus_tag="VF516_16060"
     CDS             complement(7465..8106)
                     /gene="raiA"
                     /locus_tag="VF516_16060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEX8109250.1"
                     /translation="MQFAVTFRHMEPTEALKSYARERMERVRKYLPDPISCHVVLSTE
                     RHNHRIDVTFHLHNGLSVTGHEITENMYSSIDLCIAKIERQVRKYKGKLEGMRARPHH
                     VVAQPWSHSIVSEAFGGAVAGGQSNGASHDHHEVSPSPEFKVVKTEKFHAQPLSVENA
                     IVQLNLMHEPFLVFCHDTDGRVAVVYKRDDGSYGLIETLAAPTPAAPTPASAA"
     gene            complement(8386..>9592)
                     /gene="rpoN"
                     /locus_tag="VF516_16065"
     CDS             complement(8386..>9592)
                     /gene="rpoN"
                     /locus_tag="VF516_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEX8109251.1"
                     /translation="TEIKGDATAVNEIDWENYLDNYSMAPAMPAFRGDGEELPSLEAT
                     LTRPPSLYDHLAWQLKMTNLPPDQLEIGMTILGNIDADGYIKDPPLDEIAADLGVDPT
                     LCEEVLEKIQTFDPIGVGARSLAECMLIQAVAAGQDDDLVVKIIKSHLGNLEKKNYAA
                     IARDLKQPLEEIYEAAKVIMEFDPRPGRQYSSDDPHYVTPDVYIHKVGDKYFVVPNDD
                     GLPKLKISGFYKNAMGNSAASKDYVQDKLRSAQWLIRSIQQRQRTIIKVAESILKFQR
                     EFFDKGISHLKPLILRDVAEDIGMHESTVSRVTTSKYVHTPQGIFELKFFFNSGISRT
                     NGDDLASQAVKSKIKELVDGEDVKRPHSDQKIVELLKKSGIDIARRTVAKYREQLGIL
                     SSSKRKQVF"
BASE COUNT         1378 a         3285 c         3384 g         1545 t
ORIGIN      
        1 gtgctggagt cgatattgcc ggtgaccagc gggtaggcgg cggtcggctg gtcgacggtc
       61 gcgaccggcg ccgccttcgg ccgctcggcc ggcgcttcga tcgtcgcctc ggtcgtcccc
      121 tcggccggcg cgaccaccgg cggcttgcgc tcggtcacag gctccccgtg agcaagaacc
      181 aggtctcgtt gatcccgtcg tgggcgaggc cgcgcgcgta gccgatctcg aacgtccccg
      241 gcatgaagta ccagaagaac gcgtcgagcc gcagcgcgcc gccgagcgac ccccggacgt
      301 cgcgcgcggc gttgaacgcg gtgtcgaacg cggtgccgcc atcgccgagc accgcgaggt
      361 cgacgcgccg caggtagatc gggaaggtgt cgacgccgcg ctcgatctgg aacagctcct
      421 ggcggtactc gaggttcagc aggtggtact ggttgccggc gagcacgcgc ggcggatagc
      481 cgcgcaggta gccgctgacg ccgaaccgga ggttgttgac gatcgacatc acgacgtcct
      541 gggacggcac gccgccgagc gagaacccgc cggggccgat caggtcgccc gcgcgcaccg
      601 cgccggccag gcggagcgcg aacaccgggg tcgcgcccca cagccgctgg tagatgtcca
      661 ggccgtaggc gacggtgaag ttgcgatagg tcgcaccgag cgcggggtga tcgagccgca
      721 ggctgaccgc gccgtcccag ccggcggtcg ggccgtagcc gaacgtcgtc gccttgacgc
      781 tggagtacgc gacccggctg ctgaagccgg actgcacgta gtcggtcggc gggtgggtcg
      841 gcacgcgctg ggtgggatcg agcacgaaca tcggcgcctt caccaggcgg aaccagtcga
      901 ggtcgtagtt gaacgacagc gcccagctcg actccgggcg cgactcgaac gggatgctga
      961 ccgacagcgc cccggaccag tcctcctcgg tgaacaggaa gttgcggccg tcgatccgga
     1021 atccgccgcg gtcgttcagg ctgcgcgcgc cggcgatccg caggccgggg cgcagcccgc
     1081 tgtaggcgta ggcggcgccg atgttcggcg tctgcttgtc ggtgtcggcg gccaccgcca
     1141 gcgagtacac gtgcaggccg gccgcgtcgc tgccgccggt ggtcaccgac gccgacgaca
     1201 ccgagcccag gttgtaggtg gcgctccagg tctggggcgc caggctctcg agcgcgcggt
     1261 acggccgcgg cgcggtgacg gcggcctcgc ggtcgcggat caccacggcg ggcggtttgt
     1321 cgtcgacgta gtcgcgcgcc ggttgccagc tcgccggatc gagcgggatc tcgtagaggt
     1381 cccagccgcc ggccggcacc gcggacgcga acgccaggcg ggtgccgtcg ggcgacggcg
     1441 cgggctggaa cgcgccaccg agcacgttgg tgacctgcca cagcgagcgg tcggcggtgt
     1501 cgaacgcgta gatgttctgg atcccggtgc gatcgctgtc gaacaacagg taccggccgt
     1561 cgcgggacca catcggctcc atgtcgatcg cgcggtcgtc ggtgatcgcc tcgacccgcc
     1621 cggtggcgag ctcgacgacc aggatgtcgc gcagcccgcc cttgcgccac gccgagaacg
     1681 cgatccgcgt gccgtcgggc gaccacgccg gctggtaggc ctgatcgaac cgctcgccgc
     1741 gccacaccac ggtgaccggc gcgtcgggca cgaggtcctg caccgcgagc acgctctcgg
     1801 agtgctcgtt catcgagaac gcgacccgcc ggccgtcgcg cgataccgcg gggtcgcggg
     1861 cgcggcggcc ggtcgacagc cgcaccatct gatgggtcac gccgtcccac cgcatcaggt
     1921 cctggtacga atactcgcgc cggtaggtcc gggtctgctc gaacaccagc gagccatcgc
     1981 cgagcagatc gaacggcccc atcgcgtcga tccgggcgac gtcgcgggct tggcgctgat
     2041 cgccgccgac cggcatcgcg cggacgcacg gcagcgagta gccgtcgtac tggacccagt
     2101 agatctcctt gccgtcggcc gagtagtggg gccagaagtt gctctcggcg ctgtggctca
     2161 gcgcgcggcc gaccgcgagc ccgcggcgct cggcggcggt ctcctgcagg ctgtagcggt
     2221 cgccgaggta gcgcttccag ctgtcgtaca gctcggtgaa cggcacgccg acgacccgcg
     2281 cgatctggcg gttgaccgcg tacggcggcg cgtagctgcc gccctcgtgc gccatcttgc
     2341 cgggcacgtc gtcgccgaac cgatcgaaga tgtagcgcag gaagtgcgag ccgtagacgt
     2401 agatcgcgtt gccgtgcggg aacgcgcgcg gcgcgccggt gatctcgtcg agccgcaggt
     2461 ccttgtgctc gtcgtacccg gtgcggatga actggtcgaa ccgggtgccg cggtcgcggc
     2521 cgcccgcgct gcgcttggac tcctcgtaga ccgccaggcc ctcgatgatc cagcgcggca
     2581 tgatctggtt gggcgcccag atcttgccga agatccggtt gtagatgttc ggcaggccct
     2641 ccatcgtgtc gaggtggagg atgtgggtgt actcgtgggc gatcaggccg tacagccagt
     2701 cctcgtgatc gtcgagctcg ttgaacccgg tcgggccggt cgcgaacacc tggatcatgt
     2761 tgcgcggcag cacgctggcg aacccgttgg cgctgtcggt gtcgtcgacc aggacgacga
     2821 tcgtcttgct gtccggccgg tgggcgagcg cgggcgacag cgtgcggtgc gcgcgctcgg
     2881 cgaccaccgc gagccggcgc gcgacgtcgt cgagcggcgc gtagtagtgg atcacgaagt
     2941 gatcggactc gatcgtccgc cagacgcgcg tggggtcgcc ggcgcgggcc ggggcggtca
     3001 gcgcgagcag cgccagcgcc ggcaccagcg ccagcgccgc ggccttcaca ccggtccctt
     3061 ggccccgggg tgcggggtca acccgaacac gtagtcggcg accatcgcgc ggacctccgc
     3121 ggcggtgccg agcctggcga tccgctggac cagcgcctcg gtctcggggg tccgggtcgc
     3181 gcggatcagc gccttgacct cggggaccga gaccgcgctc atcgacagct cgcggatccc
     3241 caggccgatc agcaccggcg cgatcatcgg ctcgcccgcc atctcgccgc acaccgcgac
     3301 cgggatgccg gtgctgtgag ccgcgcgggc gacctggccg agcaggcgca acagcgacgg
     3361 gtgcagcggc tcgtacaggt acgacacgta ctcgttcacc cggtcgaccg ccatcgtgta
     3421 ctggatcagg tcgttggtgc cgatcgagaa gaagtcgcac tcctcggcga ggaactcggc
     3481 ggacagcgcg gccgacggca tctcgatcat gatgccgagc ttgacgtgct cgtcgaacgc
     3541 ctggccctcc tgctggagct cgagcttgac ctcgtcgacc accgccttgg ccgcgcgcag
     3601 ctcggcgacg cccgagatca tcgggaacat gatctggagc gggccgtgcg ccgacgctcg
     3661 cagcaggccg cgcagctggg ccttgaacat cggccggccg agctgcgaca ggcacagccg
     3721 gatcgagcgc aggccgagcg ccggattgac ctccaggagc tcggcctcct cgaggaacgg
     3781 cgccagcttg tcggcgccga ggtcgtaggt ccggatcgtc gccggcttgc cgccgagccg
     3841 gcgcagcacg tcgaccgcgg tctggtagtg gcgctcctcg tcgggaggat cgtcccggtt
     3901 catgaacagg tactcggtgc ggaacagccc gacgcccatc gcgccgtagt cgagcgcgtc
     3961 ctcgatctcg tccggcccgt cgatgttgca catcagctcg atctcggcgc cgtcgagcgt
     4021 caccccgggc agatcgcgca gcgcgtggag cgtggcgctg accgcgacct ggcggcgctg
     4081 ctcgtcgcgg aactcggcga cggtcgacgg cgtcgggttg acgatcacga tgcccgagcc
     4141 gccgtcgatc agcaggcggt cgtcggtctc gaccagctcg gtgatgttct ccagcccgac
     4201 caccgcgggg atctcgtggg cgcgcgcgat gatcgcggtg tgcgaggtct tgccgccggc
     4261 gtcggtgatc atcccggcga ccgcggcctt gtggagctgc gcggtgtcgg ccggcgacag
     4321 gtcgtaggcg acgacgatcg cgtcgggcgg cggctggacc gggccggtct cgcggccgag
     4381 caggctgcgc agcacgcgct cgaacacgaa ctcgacatcc gcgcgccgcc cgcgcaggta
     4441 ctcgtcctgc atgcggtcga acaccccggc gatgtcgtcg accgcgcggc gcagccccca
     4501 ctcggcgttg atcagctcgt cgcggatgta gccgaccgcg gcgccgacca ggtgctcgtc
     4561 gtgcagcatc atctggtgcg cggtgatgat gtggtagtcg ctctcgttct cggacgcgag
     4621 ccgctccttg atccgggcga gctgcttgtc gctcgcggcg atcgccttgt acagccgcgc
     4681 gatctcggtg tcgacgtcgt cgggctcgat gtggtggcgc ggcgccttga gcgtgtcgcg
     4741 gccgatcagg taggcgcggc cgaacgcgat cccgggcgac acgccgagcc cctggcggcg
     4801 gacctcgtga cggctctcgg acatcgcggc gctacttccc ctctccgaac ttgtcctcga
     4861 tcagcgcggc cagcgtcgcg acggcctcct cggcgcggtc gccgcgcgcc cggatcgtga
     4921 tcgtcgtgcc cttgggcgcc atcagggtga gcaggctgag gatgctcttg ccgttgacct
     4981 cgttgttgcc cttgctcacg gtgatgtcgc agccggtgaa cttcgaggcc gcctggacga
     5041 acttggtccc ggcgcgcgcg tgcagcccca gctcgttctg gatctgcagc gtccgctcgg
     5101 cgcgatcgtc ggccgccatc agcccccccg atcgatgtcg cggtggcgga ccaggatgtc
     5161 ccacgcggtg cccagccgcc ggcgcagctc ctcgacgatc accaccgagc ggtggcggcc
     5221 gccggtgcaa ccgacgcaga tcgtgacgta cagcttgcct tccttgtgga actgcggcag
     5281 cgagaaccgc aggatgccct cgatctggcg gaccagctcc agcccgtcgg gcccgagcac
     5341 gtaggccgcg acctggggct cccggccgtc gaggtgggcc agcgtcggct cgaagtacgg
     5401 gttgggcagg aaccggacgt cgaacacgtg gttgagctcc tgcggcaggc cgtgcttgaa
     5461 gccgaacgac accagggtga cggcgagctt gccggtgccg ccgtagcgct cctggatgat
     5521 cgccttgagc tggtgcatgt tgaggtacga ggtgtcgatc accgccgcgc cgacgcgcag
     5581 gtcggccatc acctcgcgat cgagccggat cccctcgctg agctcgtcgc ccgacagcgg
     5641 gtggcgccgc cgggtctcgc tgaaccggcg cagcagcacc tcgtcgctgg cgtcgaggta
     5701 catcacctcc aggcggtggc ccgagccgcg caaccgggtc accacggtgg tccacgcggt
     5761 caggttgcgg cgctgccgcg agtcgatcgc gatcgcgagc ttgtcgcgat cgccctcgtt
     5821 cccgaggtgc tcgaccatct gcgcgaccag cggcacgggg atgttgtcga cgcagtagaa
     5881 ctcgatgtcc tcgagcgcgc gcagcgccgt gctcttgcct gcgcccgaca gcccggtgac
     5941 gatcacgatc tgcatctact ccaccgcgtc catcggcacg tcgcccgtcg cctcggactc
     6001 ctccatcgcc cggtgcagct gatcgcgaaa cgcgcgcgcc gagtgcgtgc cctggagctt
     6061 gagcagcagg ttgcgcgccg cgacctcgat cagggtcgcc aggttgcggc cgggccggac
     6121 cgggagctgg accgccggca ccgacacctc gaggatccgg tcgaaccgat cgtcgaaccc
     6181 gaggcggtcg tactcctcgt gctcgctcca ctcgcgcagc tcgacgacca gctcgatctt
     6241 cttggtgtcg cgcaccgccg agatcccgaa caggtccttg acgttgatga tgccgagccc
     6301 gcggatctcc atgtggtggc cgatgatccc ggcgccgcgc ccgaccacgt gccccccctg
     6361 gcggcggatc ttgatcacgt cgtcggcgac cagccggtgg ccgcggacca cgaggtcgag
     6421 cgcggtctcg ctcttgccga tcccgctctt gccgagcagc agcacgccga tcccgagcac
     6481 gtccatcagc acgccgtgca tgtcggtcga cggcgcgagc cggtcggaca tccaggcggt
     6541 gaccgcggag atgaagtccg cggtggcgag ctgcgacacc agcaccggca cgccgcgctg
     6601 ctcggcggcg ttgaccagct cgaccggcgg ggtcagcccg cggcacacca cgacgcacgc
     6661 cggatcactg gcgagcaggg tggcgatccc gagctcacgc gccggaggat gatcggcgag
     6721 gtactcgatc tcggtgccgc cgagcacgag cacgcggtgg ggatgcagct gctcgtgcca
     6781 gccggtgagc gcgagccccg gcttctggat ccgcggcacc gtcacgatcc gcgccagacc
     6841 gggctgcccc gccaccgcag tgacctccag ctctgcgcgc tccagcagct cgccgaccgt
     6901 gatcgcgctc gacattcagt acttggcgtc ctcttcggcg acgacgtggt acatcgcttc
     6961 ggcgtccgga gccgcgagca gccggcggcg aaactcgggg tccttgaaca cgcggctgat
     7021 ccgggccaga gccttgaggt gggcaccggt cgacgattcc ggcgccacca gcacgaagaa
     7081 caggtaggtc ggctgaccgt ccatcgagtc gaagtcgacc ccggtcggcg cccggccgag
     7141 gcatgcgacg atctcgccga ccgcgccgag cttgccgtgc gggatcgcca cgccctcgcc
     7201 gatcgccgtg gaggcgagct gctcgcgttc gatcagcacg cgcgacagcg tcgtgcggtc
     7261 gatccgggga tggcggcccg ccatgtagtg cgccagctcc tcgaggatgc cgcgcttgtc
     7321 cgttgcctgc agcgcgggca cgaccatgtc gcggcggatg aggtcgacga tcttcatcag
     7381 tcgaccacga acgtaccact ttctggatcc cgaggggccg gcccgaacgc ggtccagacc
     7441 cgtccgaggc ctgcaaaaac cggatcaggc ggcggaggcc ggcgtcggcg ccgcgggcgt
     7501 cggtgccgcc agcgtctcga tcaggccata gctgccgtcg tctcgcttgt agaccaccgc
     7561 gacgcggcca tcggtgtcgt ggcagaacac cagaaacggc tcgtgcatca ggttcagctg
     7621 cacgatcgcg ttctccaccg acagcggctg agcgtggaac ttctcggtct tgacgacctt
     7681 gaactcgggc gacgggctga cctcgtggtg atcgtgggac gctccgttgc tctggccgcc
     7741 cgcaacggcg ccgccgaacg cctcggacac gatcgagtgc gaccagggct gtgcgaccac
     7801 gtggtgcggc cgggcgcgca tgccctcgag cttgcccttg tacttgcgga cctggcgctc
     7861 gatcttggcg atgcacaggt cgatcgagct gtacatgttc tcggtgatct cgtgaccggt
     7921 gacggacagc ccgttgtgga ggtggaaggt gacgtcgatg cgatggttgt gacgctcggt
     7981 actgaggacg acgtggcaag agatcggatc gggcaggtac ttgcgaactc gctccattcg
     8041 ctcccgcgcg tatgacttca aggcctcggt cggttccatg tggcggaacg tcaccgcgaa
     8101 ttgcatcgag ccctccgtga tggtgtaagg ccatcatacc gccgcccaca ccggacgatc
     8161 actcgatcgt cgacgggatt tcacacgggg gtcgcgattc tactgcaatc cccacggcga
     8221 tgggcgcggc gcaatgaggc atctccagcc gaccgcgcgg gtcggtcacg gtaggtgacg
     8281 ctccccgtgg gtccacacga cccaggcgca cagtctgcgg ccgggcgcac tggccggacc
     8341 ggggcgtgcc ctgacgcgca ccgcggtgcg gcgagaaccg tggtcctaga acacctgctt
     8401 gcgcttgctc gacgacagga tgccgagctg ctcgcggtac ttcgcgaccg tgcggcgcgc
     8461 gatgtcgata ccggactttt tcaggagctc cacgatcttc tgatcgctgt gcgggcgctt
     8521 gacgtcctcg ccgtcgacca gctccttgat cttggacttc accgcctgcg aggccaggtc
     8581 gtcgccgttg gtccgcgaga tgccgctgtt gaagaagaac ttgagctcga agatgccctg
     8641 cggcgtgtgg acgtacttcg acgtggtcac ccgcgacacc gtcgactcgt gcatgccgat
     8701 gtcctcggcg acgtcgcgca ggatcagcgg cttgaggtgc gagatgccct tgtcgaagaa
     8761 ctcgcgctgg aacttcagga tcgactcggc gaccttgatg atcgtgcgct ggcgctgctg
     8821 gatcgagcgg atcagccact gcgccgagcg cagcttgtcc tggacgtagt ccttggacgc
     8881 cgccgagttg cccatcgcgt tcttgtagaa cccgctgatc ttcagcttgg gcagaccgtc
     8941 gtcgttgggc accacgaagt acttgtcgcc gaccttgtgg atgtagacgt cgggggtgac
     9001 gtagtgcggg tcgtccgacg agtactgccg cccgggccgc gggtcgaact ccatgatcac
     9061 cttggcggcc tcgtagatct cctcgagcgg ctgcttgagg tcgcgggcga tcgcggcgta
     9121 gttcttcttc tccaggttgc cgaggtggct cttgatgatc ttgaccacca ggtcgtcgtc
     9181 ctggccggcg gcgaccgcct ggatcagcat gcactcggcg agcgagcgcg cgccgacccc
     9241 gatcggatcg aaggtctgga tcttctccag cacctcctcg cacagcgtcg gatcgacccc
     9301 caggtcggcc gcgatctcgt cgagcggcgg gtccttgatg tagccgtcgg cgtcgatgtt
     9361 gccgaggatc gtcatcccga tctcgagctg gtcgggcggc aggttggtca tcttgagctg
     9421 ccacgccagg tggtcgtaca ggctcggcgg acgggtcagc gtggcttcga gcgacggcag
     9481 ctcctcgccg tcgccgcgga acgccggcat ggcgggggcc atcgagtagt tgtcgaggta
     9541 gttctcccag tcgatctcgt tgaccgcggt cgcgtcgccc ttgatctcgg tc
//
LOCUS       DASVOR010000812         3076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_241614,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000812 DASVOR010000000
VERSION     DASVOR010000812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241614"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VF516_16070"
     CDS             <1..535
                     /locus_tag="VF516_16070"
                     /inference="COORDINATES: protein motif:HMM:NF015129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109252.1"
                     /translation="ALALIAIRAVAGDLALRVRATRSVAYLHPGVAGEIVHGTDVVGW
                     FGEVHPDVRKRLGIEGAVFAFDLELEKLPLAGPAQMQPIPRFPGSTRDVSLLLAAELP
                     AARVIEVIEQVAEPLVQRIRLLEDYRDPKLGDGRKSMLWSIEYRSPDRTLTDAEVDKA
                     HEIIVGRLVENLPAQRR"
     gene            complement(551..1249)
                     /locus_tag="VF516_16075"
     CDS             complement(551..1249)
                     /locus_tag="VF516_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX8109253.1"
                     /translation="MVDDLLSGRVTAFRREQGFGVITLDDGRDVRFDASDCNCASVPE
                     EGTVVRLRVAPARWGGGLKALHVEPRGSSTLAVPASPPTLDEQIAALQREHLVGELSE
                     HVMARLSDRVFGGRIGNASLLDVLDAFYATDSSRGRHDGYLRFMPPLPSVGDALARFA
                     TALGMALPRPVACSIDDLVPQINRVLEASRDPRRVYPLATSRHWRAYYLLDTERAQRL
                     AKILPFAPTAGIAS"
     gene            1847..2137
                     /locus_tag="VF516_16080"
     CDS             1847..2137
                     /locus_tag="VF516_16080"
                     /inference="COORDINATES: protein motif:HMM:NF001401.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEX8109254.1"
                     /translation="MTKADIIEAVYEKVGGFSKKEAAEIVETVFNVVKETLERGEKIK
                     ISGFGNFVVRDKKDRMGRDPQRAVPILIPARRVLTFKPSQVLKNILNGLPPV"
     gene            2203..2276
                     /locus_tag="VF516_16085"
     tRNA            2203..2276
                     /locus_tag="VF516_16085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2237..2239,aa:Pro,seq:ggg)
     gene            2318..>3076
                     /gene="surE"
                     /locus_tag="VF516_16090"
     CDS             2318..>3076
                     /gene="surE"
                     /locus_tag="VF516_16090"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEX8109255.1"
                     /translation="MTRPLVLCSNDDGIDAPYLNALAALIEDFAEVLVVAPERQRSAA
                     SHAITLHKPLRLTEVGPRRFALSGTPVDCVYLGILKLCDRLPAVVVSGVNDGYNLGSD
                     VFYSGTVAAAVEGALRGAAGIACSLAPRAEDPDRAIRFAAAVVRAAVAEPMPAGTVLN
                     VNMPRTPSERYQWTVLGRRLYSDDVAERRDPRGRPYYWVGGGPAGHDQVEGTDCVAVA
                     RGWNSITPMHLDLTDRSRVSAPPWALEGFHVVVGS"
BASE COUNT          467 a         1097 c         1062 g          450 t
ORIGIN      
        1 ggcgctcgcg ctgatcgcga tccgcgcggt cgccggcgat ctcgcgctgc gcgtgcgcgc
       61 gacccgcagc gtcgcgtacc tccaccccgg cgtcgccggc gagatcgtcc acggcaccga
      121 cgtcgtcggc tggttcggcg aagtccaccc cgacgtccgc aagcggctgg gcatcgaggg
      181 cgcggtgttc gcgttcgacc tcgagctcga gaagctgccg ctggccggcc cggcgcagat
      241 gcagccgatc ccccggttcc cggggtcgac ccgcgacgtc tcgctgctgc tcgccgccga
      301 gctgccggcc gcgcgcgtga tcgaggtcat cgagcaggtc gccgagccgc tggtccagcg
      361 gatccggctg ctcgaggact accgcgatcc caagctcggc gacggccgca agagcatgct
      421 gtggtcgatc gagtaccgct cgccggatcg cacgctgacc gacgccgagg tcgacaaggc
      481 gcacgagatc atcgtcggcc gactggtcga gaacctgccg gcgcagcgcc gctgaccgcg
      541 gcggcgcggg ctacgacgcg atccccgcgg tgggcgcgaa cggcaggatc ttcgcgagcc
      601 gctgggcgcg ctcggtgtcg agcaggtagt aggcgcgcca gtggcggctg gtcgccagcg
      661 ggtacacccg gcgcgggtcg cgggaggcct ccagcacccg gttgatctgc ggcacgagat
      721 cgtcgatcga gcaggcgacc gggcggggga gcgccatgcc gagcgccgtg gcgaacctgg
      781 cgagcgcgtc gccgaccgac ggcagcggtg gcatgaaccg caggtagccg tcgtggcgcc
      841 cgcgggacga gtcggtggcg tagaacgcgt cgagcacatc gagcagcgag gcgttgccga
      901 tccggccgcc gaacacacgg tccgacagcc gcgccatgac gtgctcgctg agctcgccga
      961 cgaggtgctc gcgctgcagt gccgcgattt gctcgtccag ggtcggcggc gacgccggca
     1021 ctgccagcgt ggaggagcca cgcggctcga cgtgcagcgc cttgaggcca ccgccccagc
     1081 gcgccggtgc gacgcggagc cggaccacgg tgccttcttc cgggacgctc gcgcagttgc
     1141 agtccgaggc gtcgaacctg acgtcccggc catcgtcgag ggtgatcacg ccaaagcctt
     1201 gttcgcgccg gaacgcggtg acgcggccac tgagcaggtc gtccaccatg cgggcagtat
     1261 cgcctcgacg aagcgcagcg tcacgcagaa ctgtctcacg cgcgggcatt ccacacacgg
     1321 tacaatcact gcaccatcac cgaacgccgc tcgtcgcagc ctgcctacga aaatccgacg
     1381 agatgaaacg tcgatgtgac catgctcgca cgcgctgcga gcttgccgca ccgggtgcgc
     1441 accgcaccgc accgcactct cctgacgtgg cctcggatgg accccgcgat cacgtcctgc
     1501 atcgcggggc tgatggagtc aagcggcaag ggcaagggca ggggcagacg tctacgttgc
     1561 cgtcgacgcc cagggtgggg tcaaggtcca cctccaaggc gtcaacgacg gcaagcgctc
     1621 cgtcgatgtg gacggtttga cgtcgacgtg gaccgcgaac gttgccgttg atggcgacgt
     1681 acgacgtcga ctccaccgtc gacctcgtgt cctcttcctc cacatcgggg cgatcggatc
     1741 gccgttggcc caatcgtcca ttgggctaac ggggatcggc agcaattccg gtagcttact
     1801 ggcgccgccc gggcagcttc gtgataccct gggtcctatg gcgcgcatga ccaaggccga
     1861 catcatcgaa gccgtctacg agaaggtcgg cgggttctcg aagaaggaag ccgccgagat
     1921 cgtcgagacc gtgttcaacg tcgtgaagga gactctcgag cgcggcgaga agatcaagat
     1981 ctccggcttc ggcaacttcg tggttcgcga caagaaggac cgcatgggcc gcgaccctca
     2041 gcgcgcggtg ccgatcctga tcccggcgcg ccgcgtcctg accttcaagc ccagccaggt
     2101 gctcaagaac atcctcaacg gcctgccgcc cgtgtaagtg gtccggcgcg gccgtcggtt
     2161 gacgcgatgg tccggatcgg atagaccact gcagccttcg atcgaggcgt agcgcagcct
     2221 ggtagcgcac cagactgggg gtctgggggt cgcaggttca aatcctgtcg cctcgacgag
     2281 ccttggccga tcgcccccag cgatcggccc ccttttcatg acccggcccc tggttctctg
     2341 cagcaacgac gacggcatcg acgcgccgta tctcaacgcc ctggccgcgc tgatcgagga
     2401 cttcgccgag gtcctggtcg tggccccgga gcggcagcgc tcggccgcct cgcacgcgat
     2461 cacgctgcac aagccgctgc ggctcaccga ggtcggaccg cgccggttcg cgctgtcggg
     2521 caccccggtc gactgtgtct acctcggcat cctcaagctg tgcgaccggc tcccggccgt
     2581 cgtcgtgtcc ggcgtcaacg acggctacaa cctcggcagc gacgtgttct actccggcac
     2641 ggtcgccgcg gcggtcgagg gcgcgctgcg cggcgccgcc ggcatcgcct gctcactcgc
     2701 cccgcgcgcc gaggaccccg accgcgcgat ccggttcgcc gccgccgtcg tgcgcgccgc
     2761 ggtcgccgag ccgatgccgg cgggcaccgt gctcaacgtc aacatgccgc ggacgccgag
     2821 cgagcgctac cagtggaccg tgctcggccg gcggctgtac tccgacgacg tcgccgagcg
     2881 ccgcgatccg cgcggccgcc cgtattactg ggtcggcggc ggcccggccg gtcacgatca
     2941 ggtcgagggc accgactgcg tcgccgtcgc gcgcggctgg aacagcatca cgccgatgca
     3001 cctcgacctc accgatcgca gccgggtcag cgcgccgccg tgggcgctcg aaggcttcca
     3061 cgtcgtcgtg ggctcg
//
LOCUS       DASVOR010000813         3682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_504607,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000813 DASVOR010000000
VERSION     DASVOR010000813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504607"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VF516_16095"
     CDS             <1..467
                     /locus_tag="VF516_16095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109256.1"
                     /translation="SVLARAARSTRYFSVLVVGPGELSTELGGSIPASAVVTVPALTP
                     LQISQYLDAWLRATRPPCTPPLIITVDAALIAGHRANGNLDQLNALARQMIERGGPVL
                     TSWDAWTASDGRHTNGVAERPAHPAAWPTPEVLQLINHCRLAAGIAERSPPG"
     gene            complement(691..2028)
                     /locus_tag="VF516_16100"
     CDS             complement(691..2028)
                     /locus_tag="VF516_16100"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8109257.1"
                     /translation="MKAFRQLLTATLLLPGSLIVASCSSGDDAQDPKALDPGLAVATQ
                     ADDPAPGPHVNAVTEAPQGFDGLPNGNCTPAQFRDAGLTFREVEAFEDGIGPTFNNTS
                     CVSCHESPRGTAEAQDTNTSNLVFGGTGSQITELRAGHFNGISFVDHPGGSLINDRAN
                     DRSIQEHILSGNEVQTLRLTLSIAGDGFVEAIDSNTLAAIAAAQPAAQRGTLIQVPVL
                     EAPGQTRTGRFGWKDQNSSLLSFAGDAYVNEMGITNRLFQTENTSNGTNVEGGTFDGK
                     GDPSPTGEDDASDIDAFTLFMRCLKAPPRGPQNASTANGNTQFNNIGCAVCHVGTITT
                     APAGTVINGGALTVSTALGDKNIHPFSDFLLHDVGTGDGIVQNGGQGTRNQVRTAPLW
                     GIGARTRFMHDGASLTVSAAIQRHANQGATAKNNFNVLSATNQQDVINFIFSL"
     gene            complement(3443..>3682)
                     /locus_tag="VF516_16105"
     CDS             complement(3443..>3682)
                     /locus_tag="VF516_16105"
                     /inference="COORDINATES: protein motif:HMM:NF012982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HEX8109258.1"
                     /translation="NRLGVRAVEAVLAAPGAAFIHCAFGHGRSATVAAAVLVRRGDAT
                     LDDVERILRAARPRIGLNAHQRRALAEAVARMAGE"
BASE COUNT          572 a         1225 c         1239 g          646 t
ORIGIN      
        1 tctcggtgct cgcccgcgcc gcgcgcagca cgcgctactt cagcgtgctg gtggtcggcc
       61 ccggcgagct gtcgaccgag ctcggcggct cgatcccggc gagcgcggtg gtcaccgtcc
      121 cggcgctgac cccgctgcag atcagccagt acctcgacgc ctggctgcgc gcgacccgcc
      181 cgccgtgcac gccgccgctg atcatcacgg tcgacgccgc gctgatcgcc ggccaccgcg
      241 ccaacggcaa cctcgaccag ctcaacgcgc tcgcgcgcca gatgatcgag cgcggcgggc
      301 cggtgctcac gtcgtgggac gcgtggaccg ccagcgatgg ccgccacacc aacggcgtcg
      361 ccgagcggcc cgcgcacccg gccgcgtggc cgacgcccga ggtcctgcag ctgatcaacc
      421 actgccggct cgccgccgga atcgccgagc gcagcccgcc cggctgaccg gggtccggtc
      481 atcgcgtcgt gaggacgcgt ggacggccgc gacgacggcc accagacgag cgggctcacc
      541 gagccgccgg cccggctggc cgacggctcg acgggctgta gcgaatcgaa cactgccgcc
      601 actgccgcgt cgccgccggg ctggccgagc ggcccagcgg ggcatgacct cgcgccctgc
      661 ccgacgagcg acgcgcgtag cttccgggct ctacagcgag aagatgaagt tgatcacgtc
      721 ctgctggttg gtcgcgctca ggacgttgaa gttgttcttc gcggtcgcgc cctggttggc
      781 gtggcgctgg atcgcggccg acaccgtcag gctcgcgccg tcgtgcatga accgggtgcg
      841 ggcaccgatg ccccacagcg gcgcggtgcg gacctggttg cgcgtgccct ggccgccgtt
      901 ctgcacgatg ccgtcgccgg tgccgacgtc gtggagcagg aagtcgctga acgggtggat
      961 gttcttgtca ccgagcgcgg tcgacacggt gagcgcgccg ccgttgatca ccgtgcccgc
     1021 cggggcggtg gtgatggtgc caacgtggca gaccgcgcag ccgatgttgt tgaactgggt
     1081 gttgccgttg gccgtgctgg cgttctgcgg gccgcgcggc ggcgccttca ggcagcgcat
     1141 gaacagcgtg aacgcgtcga tgtcgctcgc gtcgtcctcg ccggtcgggg acggatcgcc
     1201 cttgccgtcg aacgtgccgc cctcgacatt ggttccgttg gacgtgttct cggtctggaa
     1261 cagccggttg gtgatgccca tctcgttgac gtacgcgtca ccggcgaacg acagcaggct
     1321 ggagttctgg tccttccagc cgaaccggcc ggtgcgggtc tggcccggcg cctcgagcac
     1381 cgggacctgg atcagcgtgc cgcgctgcgc ggccggctgg gcggcggcga tcgccgccag
     1441 cgtgttgctg tcgatcgcct cgacgaagcc atcgcccgcg atgctgagcg tgagccggag
     1501 cgtctggacc tcgttgcccg acaggatgtg ctcctggatc gagcggtcgt tcgcgcgatc
     1561 gttgatcagc gagccacccg ggtggtcgac gaagctgatg ccgttgaagt gaccggcgcg
     1621 gagctcggtg atctggctgc cggtgccgcc aaagaccaag ttgctggtgt tggtgtcctg
     1681 agcctccgcg gttccgcgcg ggctctcgtg gcagctgacg cacgaggtgt tgttgaacgt
     1741 cgggccaatg ccgtcctcga acgcctcgac ctccctgaac gtgaggcccg catctctgaa
     1801 ctgggccggg gtgcagttcc cgttgggtag gccgtcgaaa ccctgcggtg cctcggtgac
     1861 cgcgttgacg tgcgggcccg gcgcggggtc gtcagcctgg gtcgcgacgg cgaggccggg
     1921 atccagcgcc ttgggatcct gggcgtcgtc gcccgacgag cacgacgcca cgatcagcga
     1981 gccgggtagt agcaaggtgg ccgttaagag ctggcggaac gctttcatga cctgtttctc
     2041 ctggggttcg aaacgatgag cgggtggaca tcacccattg gacactgatg ggacccgtac
     2101 cgtattgccg agacgcccct gtgcttttgg ttccaaccaa acagcagctg gacccgccgg
     2161 ccctctcacc ccgaatctgc gcggtggacc ttacgtagcc gcgtgactca ttgtcaatca
     2221 caatgggatc gacattcgag agcttggttc tgatccttgt actgcgaacg taattggtag
     2281 ttatcactcc cgcgcacagc cctcggtcgg tccacaggtg ctcgcgatac ctactggtgg
     2341 tcaagtggat ccagaggatc gcaaatgccc ggaaccgctt cgatcacgcg atgcgtcgaa
     2401 cgccgccgtc ctgtcgcccc tcagatttcc gcggccaata tcatattggc caatttgaga
     2461 tctcagtttt ttgttgtttg gagtctcgtt gaccgcgtca aacgcagccg cgcgcggcgg
     2521 gtatggaatg gaccgttgcg gtgagcagcc cgggatctgg tcgcaatgtg acagcgttgc
     2581 gatggtccga cgcgactccc tatctggaga gctgccgatt ctgtaggagg ccgtcgggcg
     2641 ctggtcgccc ggcctcacca gctctcgagg ccaccgtaac acgcgcgatc gatcgaaccg
     2701 cgagtttttt gtagctgccg cgctgcgtgg cgtgtcccag gcgcgccctg gcgatcgagc
     2761 cgccgtccga ccggtcagca tcgatcgcgg cgcccggcag gtgaggagca gccgcgtcga
     2821 gtccctgcga gcgtgagcgc accgtgcaca gcgcggcgag tgttccacaa atggacgctc
     2881 tcgtcacgag ctgatctcga tcggctcccc gcaccgggcg gccgctgcac cggaacgccc
     2941 cactcgacgc gcgcgtcgtc gcatgcggcg cggtccggcg agctcgacct cggggtcgac
     3001 atcgcccgcg atcgggcgga atgcggccgg cgctcgtcgc ctcgtcagga agtgatcgat
     3061 cgcggagccg cgcatatcga gaactcgaga tggcgccccg gccagcggcg gtcagtcgag
     3121 tgtccgcgga tcgaccccac gagaggaggt cgtcaacgcc ggcgccaaac cggtaaagcc
     3181 gaatcgtgat ctccggcgta ctggtgatgc agccctcacg atcagcgatt tgaaatctcg
     3241 tacatggaat tacgtcataa cgaagtctta ataagacgag atttattatc tccaaataat
     3301 agtattctgg ctctcgattt tttgagttta taataaggtt cggccacccg accaatgtcc
     3361 gtctgacata aatctatata aaatcatata gttagtcttc atgaccacga ctgcacaata
     3421 tcctaccttt actgcgcaac gcttactcac cggccatgcg cgcaaccgcc tcggcgagcg
     3481 cgcggcgctg gtgggcattg agcccgatcc gcgggcgcgc cgcgcgcagg atgcgctcga
     3541 cgtcgtccag cgtggcgtca ccccggcgca ccagcaccgc ggccgcgacg gtcgccgagc
     3601 ggccatggcc gaacgcgcag tggatgaacg cagcgccggg ggccgcgagc acggcctcga
     3661 ccgcgcgcac gccgaggcgg tt
//
LOCUS       DASVOR010000814         2468 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_534060,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000814 DASVOR010000000
VERSION     DASVOR010000814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2468)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2468)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2468
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534060"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2302
                     /locus_tag="VF516_16110"
     CDS             <1..2302
                     /locus_tag="VF516_16110"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8109259.1"
                     /translation="FDDAVIAAVKAFQFEPATYGGKPVPVEITFTHTFLPPPPPPPPA
                     TDTGPPTTSVLRGRLVELGTRAPVQGATVTARVGERSYVAEADPKGHFELPLPEGPAS
                     ITVTAPGHNRFQQQESLAARQELAVTYLVERDRYDPYEFVTVGEQRREEVSRISLRGA
                     EIKQVPGTFGDPFRVVQALPGVASVVSLLPFPIVRGATPSSTGFLLDGTRVPLLYHLL
                     SGPSVIHPDFIDEIQFFPGGAPVLYGGYTGGIIDGRTARARSDEHLLDFDANLLQLGG
                     LVRQPIPQIDATATASGRYGYPGLVLSLATNQVSLSYWDYQLRLDGGNARNGWTIFAF
                     GANDELDTPAPTADPNDPNPPLAPSLILGFHRLDLRLHRTLDSVVTSFRAVLGYDKTY
                     SMGTDFSVLVAEPSWHLTWKPGDALSITTGVEGSVHKVSQGAGMAMTDNNPLSTITSQ
                     LGTFTTGGAMAEALVRPTHDWLIRPGVRADVYSDQTATRSAVDPRLTVRYRLADRELP
                     DVAPGSDDSAVWLKGAAGIYHQPPRFVLPLPGLDLMPIQYGLLRSFQTSLGVEVPLAD
                     KFQLTTEGYFNYMDPTIFDLSVNDASAVQGANASLIPTQIVLGGQRGQMLVDRLTQPQ
                     LGRAYGAEILLRRQARSGIFGWVSYTLSRSERMRDGQWVAYDFDRTHLINLVAGLPLR
                     RNWDIGLRLQYQSGKPETITAGYNDARGTGYLRFDARVDKRAVWRSWLLDFYVDITNI
                     ALLPEEVQVGTVIRYVLPTVGVRGRF"
BASE COUNT          357 a          947 c          799 g          365 t
ORIGIN      
        1 gttcgacgac gcggtgatcg ccgcggtcaa ggcgttccag ttcgagccgg cgacctacgg
       61 cggcaagccg gtgccggtcg agatcacgtt cacccacacg ttcctgccgc ccccgccgcc
      121 gcccccgccg gcgaccgaca ccggcccgcc gacgacctcg gtgctgcgcg gccggctggt
      181 cgagctcggc acccgcgctc ccgtccaggg cgccaccgtc accgcccggg tcggcgagcg
      241 cagctatgtc gccgaggccg acccgaaggg ccacttcgag ctgccgctgc ccgaggggcc
      301 ggccagcatc accgtcaccg cgcccggcca caaccgcttc cagcagcagg agtcgctggc
      361 cgccaggcag gagctcgcgg tgacctacct ggtcgagcgc gaccgctacg atccgtacga
      421 gttcgtcacg gtcggcgagc agcgccgcga ggaggtctcg cggatctcgc tgcgcggcgc
      481 cgagatcaag caggtccccg gcacgttcgg cgatccgttc cgcgtggtcc aggcgctgcc
      541 cggcgtcgcg tcggtggtgt cgctgttgcc gttcccgatc gtgcgcggcg cgaccccgag
      601 ctcgaccggg ttcctgctcg acggtacccg cgtgccgctg ctctaccacc tcctgtcggg
      661 gcccagcgtg atccaccccg acttcatcga cgagatccag ttcttcccgg gcggcgcgcc
      721 ggtgctgtac ggcggctaca ccggcggcat catcgatggc cggaccgcgc gcgcccgcag
      781 cgacgagcac ctgctcgact tcgatgcgaa tctgttacag ctcggcggcc tggtccgcca
      841 gccgatcccg cagatcgacg cgacggccac ggcctccggc cgctacggct accccggcct
      901 cgtgctcagc ctcgcgacca accaggtgtc gctgtcgtac tgggactacc agctccggct
      961 cgacggcggc aacgcgcgca acggctggac gatcttcgcg ttcggcgcca acgacgagct
     1021 cgacaccccg gcgccgaccg ccgatcccaa cgatccgaat ccgccgctcg caccgtcgct
     1081 gatcctcggc ttccaccggc tcgatctccg gctccaccgc acgctcgaca gcgtggtcac
     1141 cagcttccgc gcggtgctcg gctacgacaa gacctactcg atgggcaccg acttcagcgt
     1201 gctggtcgcc gagccgtcgt ggcacctgac gtggaagccc ggcgacgcgc tcagcatcac
     1261 gaccggcgtc gagggctcgg tccacaaggt ctcgcagggc gccggcatgg cgatgaccga
     1321 caacaacccg ctgtcgacca tcacgtcgca gctcggcacg ttcaccaccg gcggcgcgat
     1381 ggccgaggcg ctggtccggc cgacccacga ctggctgatc cgccccggcg tgcgcgccga
     1441 cgtgtactcc gaccagaccg cgaccaggtc ggccgtcgat ccgcggctca ccgttcgcta
     1501 ccggctcgcc gaccgcgagc tccccgacgt cgcgccgggc agcgacgaca gcgcggtgtg
     1561 gctcaagggc gccgccggga tctaccacca gccgccgcgg ttcgtgctgc cgctgccggg
     1621 cctggatttg atgccgatcc agtacggcct gttgcgctcg ttccagacca gcctcggcgt
     1681 cgaggtcccg ctcgccgaca agttccagct caccaccgag ggctacttca actacatgga
     1741 tccgacgatc ttcgatctgt cggtcaacga cgcgagcgcg gtccagggcg ccaacgcgag
     1801 cctgatcccg acccagatcg tgctcggcgg ccagcgcggc cagatgctgg tcgaccggct
     1861 gacccagccg cagctcggcc gggcctacgg cgccgagatc ctgctccgcc gccaggccag
     1921 gtccgggatc ttcggctggg tgtcgtacac gctgtcgcgc tccgagcgca tgcgcgacgg
     1981 ccagtgggtg gcgtacgact tcgatcgcac ccacctgatc aacctggtcg ccggcctgcc
     2041 gctgcgccgc aactgggaca tcggcctgcg cctgcagtac cagagcggca agcccgagac
     2101 catcaccgcc ggctacaacg acgcgcgcgg caccggctac ctgcggttcg acgcccgggt
     2161 cgacaagcgc gcggtgtggc ggagctggct gctcgacttc tacgtcgaca tcaccaacat
     2221 cgcgctgctg ccggaggagg tccaggtcgg gaccgtgatc cgctacgtgc tgccgaccgt
     2281 cggcgtgcgc ggccggttct agcgatgcca gcacatccgg aacgacaccc aggagacgag
     2341 atgaccaacg acacgatcga tgcccagctc ctcgacctca tgctgcgcgc cggctcgcgc
     2401 tgggtgctct ggctcttgct ggcgctcagc ctcgccgcgg tcgcggtcat gatcgaccgg
     2461 atctggtt
//
LOCUS       DASVOR010000815         1529 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_212637,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000815 DASVOR010000000
VERSION     DASVOR010000815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212637"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VF516_16115"
     CDS             <1..326
                     /locus_tag="VF516_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109260.1"
                     /translation="SLVAALGVDPDRLLASAAPSPGEARKLALALGLGRHAWCLVLDE
                     PTNHLDLPSIERLEEALVAYPGALVLVSHDDRFAARCTATTWRVAGGKVDTAPVSESA
                     ASLAI"
     gene            complement(359..>1529)
                     /locus_tag="VF516_16120"
     CDS             complement(359..>1529)
                     /locus_tag="VF516_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEX8109261.1"
                     /translation="RDPFELLDEIAHDLQLDVVPASWPIGMGREFRGCYDLFADQLIL
                     MERTRGEYVQEGITVAGLADPRLDELLPDTAVAKLREDVEMVRGLCPKFDLESYRAGH
                     LTPVFFGSAVNNFGVRELLAGVARLAPPPRPQPAREHPVDPTDAKVSGFVFKIQANMD
                     PKHRDRIAFVRLASGHFTRGMRLTVPRTGKALGVHNAMLFQANERELAEEAWAGDIIG
                     IPNHGTLRIGDALTEGEILHFTGIPSFAPEFLRRVRADDPMKAKHLGRALEQIAEEGG
                     AQVFKMFLGSDFVVGVVGALQFEVLADRIRSEYDLPCHFEATSFEVARWVDADDPKLI
                     KRFADANRDNIAEDHSGATVFLARNDWQLNRARQDFPQLRFLQTREQAPLTAASA"
BASE COUNT          209 a          505 c          592 g          223 t
ORIGIN      
        1 tgtcgctggt cgcagcgctc ggcgtcgatc ctgatcgcct gctcgcctcc gccgcgccat
       61 cgcccggcga ggcccgcaag ctcgccctcg cgctcggcct gggccgccac gcctggtgcc
      121 tcgtgctcga cgagccgacc aaccacctcg acctgccgtc gatcgagcgc ctggaagaag
      181 ccctggtcgc ctatcccggc gcgctcgtcc tggtcagcca cgacgaccgg ttcgccgccc
      241 gctgcaccgc gaccacctgg cgcgtggccg gcgggaaggt cgacacggcg ccggtcagcg
      301 aatcggcggc atcgctcgcg atctaaggac ggtccgcgat ggaaccgtcg ccgccgactc
      361 acgccgaggc ggcggtcagc ggggcctgct cgcgggtctg gaggaagcgg agctggggga
      421 agtcctgccg ggcgcggttg agctgccagt cgttgcgggc gaggaacacg gtggcgccgg
      481 agtggtcctc ggcgatgttg tcgcggttgg cgtcggcgaa ccgcttgatc agcttgggat
      541 cgtcggcgtc gacccagcgc gcgacctcga acgaggtggc ctcgaagtgg cagggcaggt
      601 cgtactcgga gcggatccgg tcggcgagga cctcgaactg cagggcgccg accacgccga
      661 cgacgaagtc cgagccgagg aacatcttga acacctgggc gccgccctcc tcggcgatct
      721 gctcgagggc gcggccgagg tgcttggcct tcatcgggtc gtcggcgcgg acgcggcgca
      781 ggaactcggg ggcgaagctc gggatgccgg tgaagtgcag gatctcgccc tcggtgagcg
      841 cgtcgccgat ccgcagggtg ccgtggttgg ggatgccgat gatgtcgccg gcccaggcct
      901 cctcggcgag ctcgcgctcg ttggcctgga acagcatggc gttgtggacg ccgagcgcct
      961 tgccggtgcg cggcacggtg agccgcatgc cgcgggtgaa gtggccggag gcgaggcgga
     1021 cgaacgcgat gcggtcgcgg tgcttggggt ccatgttcgc ctggatcttg aagacgaagc
     1081 cggagacctt cgcgtcggtc gggtcgaccg gatgctcgcg cgcgggctgc gggcggggcg
     1141 gcggggcgag ccgggcgacg ccggcgagca gctcgcggac gccgaagttg ttgaccgcgc
     1201 tgccgaagaa caccggggtg aggtggccgg cgcggtacga ctcgaggtcg aacttcgggc
     1261 acagcccgcg gaccatctcg acgtcctcgc gcagcttggc gaccgcggtg tcggggagca
     1321 gctcgtcgag cctggggtcg gccaggccgg cgacggtgat gccctcctgg acgtactcgc
     1381 cgcgggtacg ctccatcagg atcagctggt cggcgaacag gtcgtagcag ccgcggaact
     1441 cgcggcccat gccgatcggc cagctcgccg gcacgacgtc gagctggagg tcgtgggcga
     1501 tctcgtcgag cagctcgaac ggatcgcgg
//
LOCUS       DASVOR010000816         4237 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_183559,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000816 DASVOR010000000
VERSION     DASVOR010000816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_183559"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..926)
                     /locus_tag="VF516_16125"
     CDS             complement(6..926)
                     /locus_tag="VF516_16125"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8109262.1"
                     /translation="MARLLWRDAQGIEGSLDLTSAEIRIGRAMDCAIRTDDAMVSRHH
                     SRVFWGGGGYILEDLSSANGVYFQEQRVQSHLFKHGDAVRCGSLWLRFVDTGPSADGG
                     GAGQPPPMIPSHPGMGAVVPIAQTGHVSALQSQPQPPAQPPPQPPPAPPASTGPSEAD
                     VEIRVLRRRVEQLQTELRVYRTNKFNQDTARRMEDLENDLSMMEIERDNLKAKLERAE
                     QQLTLEAGSVKVRRALEIAAATAETAASLNDLLSSLRIEVMAAEGEFEQYSQSIPRAS
                     FELIRQSLKDGAGHCDEARELLRKLREVAE"
     gene            1007..2416
                     /gene="purF"
                     /locus_tag="VF516_16130"
     CDS             1007..2416
                     /gene="purF"
                     /locus_tag="VF516_16130"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEX8109263.1"
                     /translation="MCGVFGIHGHDEAANIAYLGMHALQHRGQESAGLVAVEASEGRL
                     RRQAAMGLVSEAFNRETLAKLPGSVAVGHVRYSTAGGSELRNAQPFVVEYAGGQLAIC
                     HNGNLLNAQELRAELEASGSIFQTSSDTEVIVHLMAKAREADVLGRLMAALRRVRGAY
                     SLICLTHDARMIGVRDPNGFRPLVIGRLKDAFVLSSETCAFDLIEAEFIREVEPGEIV
                     VVDKGGMTSHRLPDTAPPSFCVFEHVYFARPDSLVNGKSVYRVREKLGRRLAQEAPVV
                     ADVVIPVPDSGVPSAIGYANEAKIPFEMGLIRSHYVGRTFIEPQDSIRHFGVRLKLSP
                     VRSIVDGKRVVVVDDSLVRGTTSRKIIKMLRNAGAREVHLRISAPPTTHPCYYGIDTP
                     HRSELVAASHTVEEIGRYVTCDSIAYLSHEGLMAAVGAETGSAKGSAGTGYCSACFTG
                     KYPVALGTPDLVQLRRARV"
     gene            2508..3551
                     /gene="prfB"
                     /locus_tag="VF516_16135"
                     /pseudo
     CDS             2508..3551
                     /gene="prfB"
                     /locus_tag="VF516_16135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00020.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            3823..>4237
                     /locus_tag="VF516_16140"
     CDS             3823..>4237
                     /locus_tag="VF516_16140"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8109264.1"
                     /translation="MYKMIAFSALAALCVAGCNKKKDEGGGSAAAMTKEDKGPAKGPE
                     SAGAVTINGSGSTFQKQFQEVAIEGFSKASKDVKVNYGAGGSGKGRQDFSDMVTDYGC
                     TDGLFKDADAAKAKGGAFLYFPILLGAITVSYNLDG"
BASE COUNT          705 a         1346 c         1566 g          620 t
ORIGIN      
        1 cgcggctact cggcgacctc gcggagcttg cggagcagct cgcgggcctc gtcgcagtga
       61 cccgcgccgt ccttgagcga ctggcggatc agctcgaagc tggcgcgcgg gatgctctgc
      121 gagtactgct cgaactcgcc ctcggcggcc atgacctcga tgcgcaggct cgacagcagg
      181 tcgttgagcg aggccgcggt ctcggcggtg gcggcggcga tctcgagcgc gcggcggacc
      241 ttgacgctgc cggcctcgag ggtgagctgc tgctcggcgc gctcgagctt ggccttgagg
      301 ttgtcgcgct cgatctccat catcgagagg tcgttctcga ggtcctccat ccggcgggcg
      361 gtgtcctggt tgaacttgtt ggtgcggtag acgcggagct cggtctgcag ctgctcgacg
      421 cggcggcgca ggacccggat ctcgacgtcg gcctcgctgg ggccggtgct ggccggcggg
      481 gcgggcggcg gctgcggcgg gggctgagcc gggggctggg gctgggactg cagcgccgag
      541 acgtggccgg tctgggcgat cgggacgacc gcgcccatgc cggggtggga cgggatcatc
      601 ggcgggggct ggccggcgcc gccgccgtcg gcggacgggc cggtgtcgac gaaccgcagc
      661 cacaggctgc cgcagcgcac ggcgtcgccg tgcttgaaca ggtggctctg gacccgctgc
      721 tcctggaagt agacgccgtt ggccgagctc aggtcctcga ggatgtagcc gccgccgccc
      781 cagaacaccc gcgagtggtg gcgggagacc atggcgtcat cggtgcggat ggcgcagtcc
      841 atcgcgcggc cgatccggat ctccgccgag gtgaggtcga gggaaccctc gataccctgt
      901 gcatcgcgcc agaggagccg ggccacgtgt ctcaaaaagg taagccagac cggagagatc
      961 ctcaagttcg ctactgagga tcacaccgcc atggtaagtg agcgccatgt gcggcgtctt
     1021 cggcatccac ggccatgacg aggcggcaaa catcgcctac ctcggcatgc acgcgcttca
     1081 gcatcggggg caggagtcgg ccgggctggt ggcggtcgag gccagcgagg gccggctccg
     1141 gaggcaggcg gcgatgggcc tggtttcgga ggctttcaat cgcgagaccc tggccaagct
     1201 gccgggaagc gtggcggtcg gccatgtccg gtactcgacc gccggggggt cggagctgcg
     1261 caacgcccag ccgttcgtgg tcgagtacgc cggcggccag ctggcgatct gtcacaacgg
     1321 caacctcctg aatgcacagg agttacgggc cgagctggag gcgagcggct cgatcttcca
     1381 gacctcgagc gacaccgagg tcatcgtcca tctgatggcc aaggcccgag aggcggacgt
     1441 gctgggccgg ctgatggcgg cgctgcgccg ggtgcgcggc gcgtactcgc tgatctgcct
     1501 gacccacgac gccaggatga tcggcgtgcg cgacccgaac gggttccggc cgctggtgat
     1561 cggccggctc aaggacgcgt tcgtgctgtc gagcgagacc tgcgcgttcg acctgatcga
     1621 ggccgagttc atccgtgagg tcgagccggg cgagatcgtg gtggttgaca agggcgggat
     1681 gacgtcgcac cggctgccgg acaccgcccc gccgtcgttc tgcgtgttcg agcacgtgta
     1741 cttcgcccgg cccgacagcc tggtgaacgg caagtcggtc taccgggtgc gcgagaagct
     1801 cggccggcgg ctggcgcagg aggcgccggt ggtcgccgac gtggtgatcc cggttccgga
     1861 ttccggagtg ccgtcggcga tcgggtatgc caacgaggcc aagatcccgt tcgagatggg
     1921 gctgatccgg tcgcactacg tcggccggac gttcatcgag ccgcaggact cgatccgaca
     1981 cttcggcgtg cggctcaagc tgtcgccggt gcgctcgatc gtcgatggca agcgcgtcgt
     2041 cgtggtcgac gactcgctgg tccgcggcac gacgtcgcgc aagatcatca agatgctgcg
     2101 caacgcgggg gcccgcgagg tccacctccg gatcagcgcg ccgccgacga cgcacccgtg
     2161 ctactacggc atcgatacgc cgcatcgtag cgagctggtc gcggcatcgc acacggtcga
     2221 ggagatcggg cgctacgtga cctgtgactc gatcgcgtac ctgtcgcacg agggcctgat
     2281 ggcggcggtg ggcgccgaga cggggtcggc gaagggcagt gccgggactg gatactgctc
     2341 ggcgtgcttc acaggtaagt acccggttgc actgggaacg ccggaccttg tccagctccg
     2401 ccgggccagg gtatagagcg ccatgggttt ggaagctgta gatgtccgcg ccgtgcgtgc
     2461 gcagctgaag gagctcggcc agaaggccca ggagctccgg aggttacttt gacgtcgacc
     2521 agaagcgcct gaagatcgag gagctcgatt cgatcgccgc gaagccgacg ttctgggacg
     2581 accagaagaa ggcgcagacg ctcttgcgca gcaagaagga gctcgaggcg atcgtcggcg
     2641 gctacgacgc gcaggtccgc gcgctgtcgg acgccgaggt cctgctcgag ctcgccgagg
     2701 aggccagcga cgaggcgtcg gcgcgcgagg tcgagtccag cgtcaccgcc gtccgcaagg
     2761 cgctcgacga catcgagttc aagcgcatgc tgtcgggcga gctcgacagc ggcggggcga
     2821 tcctccagat cgtctcgggg gccggcgggg tcgatgcgtc ggactgggcg cagatgctga
     2881 tgcgcatggt gatccggtac gccgagcgca agggctggaa ggtcgagatc ctcgaggaga
     2941 cgcgggccga ggaggccggg atcaagagcg cgacgctcac cgtggccggc gactacgcct
     3001 acggcctgct caaggccgag gacggcgtgc accggctggt ccgggtgtcg ccgttcgaca
     3061 ccaatggccg gcgccagacg tcgttcgcgg cgatccgggt cacgccggac atcgacgaca
     3121 acatcgaggt cgagatcgac gaggccgacc tcaagatcga cacctaccgg gccggcgggg
     3181 ccggcggcca gcacgtcaac aagaccgact cggcggtgcg gatcacgcac ctgccgacga
     3241 agatcgtcgt gcagtgccag aacgagcgca gccagcactc gaacaaggcc aaggcgatga
     3301 agctgctgcg cgcgcggctg tacgactacg agctcgccaa gcgcgaagcc aaggccgccg
     3361 cggacgccgc ggcccggctc gacgtcgagt ggggctcgca gatccgcagc tacgtgctgt
     3421 tcccgtacca gcaggtcaac gaccaccgca ccgagctcaa gacctcggac gtcgacggtg
     3481 tgctcgacgg cgatctcgac gacctgatcc gcacctacct gctcagggca gcggatcagc
     3541 cgaagaacta agcccgcggc gcgaagcgac gcgcccggct gcgaagcaga cgggagacct
     3601 gcgaagcagg tcaggcgtga ctcggcgata gccgagaact gaccgccgcc cgtggcgagc
     3661 cgtattcagt gtagtgcctg cgcggacccg cgcggcacca gggcgcacgc cgagcgccga
     3721 tcacccagcc gcgaccctgt gagtcagcgc tcttgcccga ccgtcacagt ccggtcacag
     3781 acggtgcgca gatgcgtcac gaaggctgct tacggtcacg gcatgtacaa gatgatcgcg
     3841 ttctcagccc tggcggcgct gtgcgtggcc gggtgcaaca agaagaagga cgagggtggg
     3901 ggcagcgccg ctgcgatgac caaggaggac aagggtccgg ccaagggtcc ggagtccgcc
     3961 ggtgcggtca cgatcaacgg cagcgggtcg accttccaga agcagttcca ggaggtcgcc
     4021 atcgagggat tctcgaaggc cagcaaggac gtgaaggtga actacggcgc cggcggctcg
     4081 ggcaaggggc gtcaggactt ctccgacatg gtcaccgact atggatgcac cgacggcctg
     4141 ttcaaggacg ccgacgcggc caaggccaag ggcggcgcgt tcctgtactt cccgatcctg
     4201 cttggcgcga tcacggtgag ctacaacctc gacggcg
//
LOCUS       DASVOR010000817         2615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_37192,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000817 DASVOR010000000
VERSION     DASVOR010000817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2615
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37192"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..2173
                     /gene="speA"
                     /locus_tag="VF516_16145"
     CDS             185..2173
                     /gene="speA"
                     /locus_tag="VF516_16145"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEX8109265.1"
                     /translation="MARAKALIAHDPLRRWTAADANDTYNIPRWGCGYFGVNEQGNLI
                     VTPRPNGDASAAAPNVVQLGDASSGGAIDMKELIDELVARGIQLPILLRFSDILRARI
                     ELLCGAFNAAIREYGYAGQYRGVYPIKVNQNRTVVEEIIEFGRPYHYGLEAGSKPELL
                     AIMAIHQDDESIIICNGYKDEEYIETALLASKLGKTVILVIEKPSELEHIHRVAERVK
                     IKPSLGIRARLSSRGAGRWEQSGGDFSKFGLSAAEMVDAVQKLKDWGEVDALKLIHFH
                     LGSQISAIKSIKNALREAGRLFVEMYKMGAKGLSYLDVGGGLGVDYDGSQTNFASSMN
                     YTIQEYANDVVYAIKEICDADQVPHPNIVSESGRAVAAHHSVLVVNVLGVTEFNTNVP
                     RELQRPAPPLVTNLWETYQSVSAKNLLEAYHDAVEYKDQVLQLFNLGHLSLEHRVLCE
                     NLFWATCEKVLMAVRNRDHLPEELEGLEKTLSDTYFCNFSMFQSVPDAWAVDQLFPIC
                     PIHRLNEEPTRRATLADITCDSDGKIDSFIDLRDVKHVLELHPKHDDQDYYLGIFLVG
                     AYQEILGDLHNLFGDTNTVHVQLSPEGDYDVKEVVAGDTVSEVLAFVDYSPSDLLGRL
                     RNSVEQALRKKLMTIEESRTLINRFRQGMIGYTYLDRE"
     gene            complement(2209..>2615)
                     /locus_tag="VF516_16150"
     CDS             complement(2209..>2615)
                     /locus_tag="VF516_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109266.1"
                     /translation="AAGLTGAFRALLATAAMPAAARFDPAAGPVVRPDGIEIAASVGD
                     LLAGKLASAANATLDGRYLSGPVWTGAESPTSAQGAPCSDWGKSDASALAMVGELASA
                     QAGFFGLIDLSGFHVGRNKPCNSSFPVYCLEH"
BASE COUNT          451 a          897 c          871 g          396 t
ORIGIN      
        1 tgtaggtgtc agtcgcgtcg gcggcggtcc accggcggag cggatcggtc gagagcaatg
       61 ccttcgcgcg ggccatgatc tttgatcatg ggattacgtg catttgcccc cccgcgcaat
      121 ggggatctgc gcgcggtttc ccgttgcacc ggcggtcaat tgtgcgtaag tgcatgttgg
      181 aaccatggcc cgcgccaagg cactgatcgc ccacgacccg ctccgccggt ggaccgccgc
      241 cgacgcgaac gacacctaca acatcccgcg ctggggctgc gggtacttcg gggtcaacga
      301 gcagggcaac ctgatcgtca cgccgcggcc gaacggcgac gccagcgcgg cggcgcccaa
      361 cgtggtccag ctcggcgatg cctcgtccgg cggcgcgatc gacatgaagg agctgatcga
      421 cgagctggtc gcgcgcggca tccagctgcc gatcctgctc cggttcagcg acatcctgcg
      481 ggcgcggatc gagctcttgt gcggcgcgtt caacgccgcg atccgcgagt acggctacgc
      541 cggccagtac cgcggcgtgt acccgatcaa ggtcaaccag aaccgcaccg tcgtcgagga
      601 gatcatcgag ttcggccggc cctaccacta cgggctggag gccggcagca agcccgagct
      661 cttggcgatc atggcgatcc accaggacga cgagtcgatc atcatctgca acgggtacaa
      721 ggacgaggag tacatcgaga ccgcgctcct ggcctcgaag ctcggcaaga cggtgatcct
      781 cgtgatcgag aagccgagcg agctcgagca catccaccgg gtcgccgagc gggtgaagat
      841 caagccgtcg ctcgggatcc gggcgcggct ctcctcgcgc ggcgccgggc ggtgggagca
      901 gtcgggcggc gacttctcca agttcgggct gtccgcggcc gagatggtcg acgcggtcca
      961 gaagctcaag gactggggcg aggtcgacgc gctcaagctg atccacttcc acctcggctc
     1021 gcagatctcg gcgatcaaga gcatcaagaa cgcgttgcgc gaggccggcc ggctgttcgt
     1081 cgagatgtac aagatgggcg ccaagggcct gtcgtacctc gacgtcggcg gcgggctcgg
     1141 cgtcgactac gacggctcgc agaccaactt cgcgtcgtcg atgaactaca cgatccagga
     1201 gtacgccaac gacgtggtct acgcgatcaa ggagatctgc gacgccgacc aggtcccgca
     1261 ccccaacatc gtgagcgagt ccggccgcgc ggtcgccgcg caccactcgg tgctggtggt
     1321 caacgtgctc ggcgtgaccg agttcaacac caacgtgccc cgcgagctgc agcgcccggc
     1381 gccgccgctg gtcaccaacc tgtgggagac ctaccagagc gtcagcgcca agaacctgct
     1441 cgaggcctac cacgacgcgg tcgagtacaa ggaccaggtg ctccagctgt tcaacctcgg
     1501 gcacctgtcg ctggagcacc gcgtgctgtg cgagaacctg ttctgggcga cctgcgagaa
     1561 ggtgctgatg gcggtgcgca accgcgacca cctgcccgag gagctcgagg ggctcgagaa
     1621 gacgctgtcc gacacctact tctgcaactt ctcgatgttc cagtcggtgc ccgatgcgtg
     1681 ggcggtcgac cagctgtttc cgatctgccc gatccaccgg ctcaacgagg agccgacccg
     1741 gcgcgccacg ctggccgaca tcacgtgcga ctccgacggc aagatcgact cgttcatcga
     1801 cctgcgcgac gtcaagcacg tgctggagct ccaccccaag cacgacgacc aggactacta
     1861 cctcggcatc ttcctggtcg gcgcctacca ggagatcctc ggcgacctcc acaacctgtt
     1921 cggcgacacc aacaccgtgc acgtccagct gtcgcccgaa ggcgactacg acgtcaagga
     1981 ggtggtcgcc ggtgacacgg tcagcgaggt gctggcgttc gtcgactact cgccgtccga
     2041 cctgctcggc cggctgcgca acagcgtcga gcaggcgctg cgcaagaagc tgatgacgat
     2101 cgaggagagc cgcacgctga tcaaccggtt ccgccagggg atgatcggct acacgtacct
     2161 cgatcgcgag tagcgcgtgc tgcacctgcg cccggccggc tgtcgccgtc agtgctcgag
     2221 gcagtagacc ggaaagctgc tgttgcacgg cttgttgcgg ccgacgtgga agccgctgag
     2281 gtcgatgagg ccgaagaagc cggcctgcgc cgacgccagc tcaccgacca tcgcgagcgc
     2341 gctggcgtcg ctcttccccc agtcgctgca cggggcgcct tgcgccgacg tcgggctctc
     2401 ggcgcccgtc cacaccgggc cgctgaggta gcggccgtcg agcgtcgcgt tggcggcgct
     2461 cgccagcttg cccgcgagca gatcgccgac gctggcggcg atctcgatgc cgtcgggccg
     2521 caccaccggt cccgcggccg ggtcgaaccg cgccgcggcc ggcatcgccg cggtcgcgag
     2581 cagcgcacga aacgccccgg tgaggccagc tgccg
//
LOCUS       DASVOR010000818         1646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_241690,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000818 DASVOR010000000
VERSION     DASVOR010000818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1646
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241690"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..476)
                     /locus_tag="VF516_16155"
     CDS             complement(<1..476)
                     /locus_tag="VF516_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109267.1"
                     /translation="MSGPKRNPDRLNTIGVVVVGICGAVLVYVTIVALQAFYVNDTSE
                     IQTMADYGGQDTAAKSLRTDQLSRINEYGTNPRPAAAKAGEAQRPQTYRVPIDVAMKR
                     VVEDARADPGALVPSLGRSDKATVLPIFGRPKLIPAPAAAAPAGAAPAAGAGSGATA"
     gene            complement(489..>1646)
                     /locus_tag="VF516_16160"
     CDS             complement(489..>1646)
                     /locus_tag="VF516_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109268.1"
                     /translation="HVDHATGEPAKWARFLYDYVIGWSFIFSICLGVLWLILLHFLVR
                     GRWATVVRRIAEAMASALPWVFASGLVFVIPLLAGYDNLYFWAHPDAAKDPTLAFKLG
                     WLSPGFFAVRYVFYAVVFSAMAAYFARKSRQQDESGDPKISESLRVASGPAMVVYSLA
                     LCLAAFDILMSMAPRWYSTIYGVNFWGGACVGGFSALALMVLGIQRTGRLTHSVTPEH
                     YHDIGKWMFAFTFFWAYTAFSQFMLQWYGNMPEETVWYKYRLFGDWTWVSIAILVGFW
                     AFPFVFLMSRWTKRIVPSLVFFAVWQLVFHWLDLYWNVMPSYDWAREAHERGPLTGAI
                     AYHHVGFSPLDITVWFALIGVLLFAIGRNLRGNLIPVKDPTLGLSLAHENL"
BASE COUNT          300 a          565 c          558 g          223 t
ORIGIN      
        1 gcggtggctc cgctgccggc accggcggcg ggcgctgccc cagcgggcgc agccgcggcg
       61 ggcgccggga tcagcttggg acggccgaag atcggcagca cggtcgcctt gtccgagcgg
      121 ccgagcgatg gcaccagcgc gcccggatcg gccctggcat cctcgacgac gcgcttcatc
      181 gcgacgtcga tcggcacccg gtaggtctgc ggccgctgcg cctccccggc cttggccgcc
      241 gccggccgcg ggttggtgcc gtactcgttg atccggctca gctggtcggt ccgcagcgac
      301 ttcgcggcgg tgtcctggcc gccgtagtcg gccatggtct ggatctccga ggtgtcgttc
      361 acgtagaacg cctggagcgc gacgatcgtg acgtacacca gcacggcgcc gcagatcccg
      421 acgacgacga cgccgatcgt gttcagccgg tctggatttc gcttgggtcc cgacatggcg
      481 attggctcct agaggttctc gtgggcgagc gacaggccca gcgtcggatc cttgaccggg
      541 atcaggttgc cgcgcaggtt gcggccgatc gcgaacaaga ggacgccgat cagggcgaac
      601 cacacggtga tgtcgagcgg cgagaacccg acgtggtgat acgcgatggc gccggtcaac
      661 gggcccctct cgtgggcctc ccgcgcccag tcgtacgacg gcatcacgtt ccagtacagg
      721 tcgagccagt ggaacacgag ctgccacacc gcgaagaaca ccagcgacgg cacgatccgc
      781 ttggtccagc gcgacatcag gaacacgaac gggaacgccc agaacccgac gaggatcgcg
      841 atgctcaccc aggtccagtc gccgaacagc cggtacttgt accacaccgt ctcctcgggc
      901 atgttgccgt accactgcag catgaactgc gagaacgcgg tgtaggccca gaagaacgtg
      961 aacgcgaaca tccacttgcc gatgtcgtgg tagtgctcgg gggtgaccga gtgggtcagc
     1021 cgcccggtgc gctggatgcc gagcaccatc agcgcgagcg cggagaaccc gccgacacac
     1081 gcgccgcccc agaagttgac gccgtagatc gtcgagtacc acctgggcgc catcgacatc
     1141 aggatgtcga acgccgccag gcagagcgcc agcgagtaga cgaccatcgc cggccccgac
     1201 gcgacccgca ggctctcgga gatcttggga tcgccggact cgtcctgctg gcgcgacttg
     1261 cgcgcgaagt acgccgccat cgccgagaac accacggcgt agaacacgta gcgcaccgcg
     1321 aagaacccgg gcgacagcca gccgagcttg aacgccagcg tcgggtcctt cgccgcgtcc
     1381 gggtgcgccc agaagtacag gttgtcgtag ccggcgagca gcgggatcac gaacaccagc
     1441 ccggacgcga acacccaggg cagcgcgctc gccatcgcct cggcgatccg gcgcaccacg
     1501 gtcgcccacc ggccgcgcac caggaaatgg agcaggatca gccagagcac gccgaggcag
     1561 atgctgaaga tgaacgacca gccgatcacg tagtcgtaga ggaaccgggc ccacttcgcg
     1621 ggctcgccgg tcgcgtggtc gacgtg
//
LOCUS       DASVOR010000819         3632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_446124,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000819 DASVOR010000000
VERSION     DASVOR010000819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446124"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1110
                     /locus_tag="VF516_16165"
     CDS             <1..1110
                     /locus_tag="VF516_16165"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8109269.1"
                     /translation="AAVTVPVFILPGHVDRCISLELGYGRWAAGPVGDGVGSNVYPLR
                     TSTRPWFAPAAAIAATAERTPLAVTQEHWRQHDRQIAMAAALDDYRRDPDFTRERWQP
                     EQTMLPPQFENFFQWGMTIDTMICSGCSACVIACQAENNVPSVGAEDVRRNREMHWLR
                     IDLYREGSEDDPRYINQPMLCQHCANAPCEYVCPVYATQHSPDGLNEMIYNRCIGTRF
                     CSNNCPYKVRRFNWFNFTRDTPPTRQLQYNPNVTVRDRGVMEKCTYCVQRIRAADIAV
                     RKQHRDIRPGEVVTACQQACPTGAIQFGRLDHDQTLNVQWRGESRHYAVLSQLGTRPR
                     TVYLAKIWNPSPDLPMQLGEGDPERDPERDPEGDR"
     gene            1107..2489
                     /gene="nrfD"
                     /locus_tag="VF516_16170"
     CDS             1107..2489
                     /gene="nrfD"
                     /locus_tag="VF516_16170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX8109270.1"
                     /translation="MTQPLLIGRPSDAALGDELLGTTWRAGGTGWKIAFAITGALTGL
                     LIALLTYAMATGIGVFGNNIPVAWAFPIINFVWWIGIGHAGTFISAILYLFEQGWRTS
                     INRLSEAMTLFAVLQAAIFPVLHLGRPWFAYWLFPYPSLMEVWPQVKSALPWDAAAVS
                     TYFTVSLLFWYLGLVPDFASLRDSAPTRRRRAIYGLFALGWNGSATTWRHYKAVYLVL
                     AGLATPLVLSVHSVVSSDFAIALVPGWHSTIFPPYFVAGAIFSGFAMVLTLAIPTRRV
                     FGLHNVITTKHLENCAKLIMVTGWIVAYGYMMEWFLDWYSGDPSEMYQAFVARPFGPN
                     KVVFYLSMICNVGVPQIFWWKRARLNVWILWFAALLINVGMWSERMMIIVQSLQREFL
                     PSQWGDYSPTWVDIGILFGTVGFFTFLFLLFLRFVPFISLSEMREMTHELVKHERGAG
                     APHPEEHPHG"
     gene            2482..3009
                     /locus_tag="VF516_16175"
     CDS             2482..3009
                     /locus_tag="VF516_16175"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEX8109271.1"
                     /translation="MADRILAQYPHEDALVAAIHRLRDKGYRALEAYLPFPAIGVEEA
                     LTTPSRLPYLCFFFGILAAATAYALQWLLNGYLYPLVVGGRPPHFPLTFVPITFEMGV
                     LFASFTAFLGLLWRGRLVRLTDPIQGTPGFDSATRDRFWLEVSAADPAFDLDRTRADL
                     VETGALRIELPEVGP"
     gene            3006..3602
                     /locus_tag="VF516_16180"
     CDS             3006..3602
                     /locus_tag="VF516_16180"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8109272.1"
                     /translation="MIRALITVALCGLTGCGGCSPWDLDLERMIEQPKYTTYQACEVC
                     PGGTIMMPPPAGTMPRGEPVAPTPLVLGREAGRTLDRFPVAVTRGVLDRGRNRFDIFC
                     AACHGRLGNGVSKVAENMTLRRPANLLTAPYTEYPPGRIFNTITQGYGLMRSYAGELP
                     IADRWAVVAYVQALQLSQHVELDALPAHDRSEASRWLK"
BASE COUNT          524 a         1292 c         1215 g          601 t
ORIGIN      
        1 gccgcggtga ccgtgccggt gttcatcctg cccggccacg tcgatcgctg catcagcctg
       61 gagctcggct acggccggtg ggccgcgggg ccggtcggcg acggcgtcgg cagcaacgtc
      121 tacccgctgc gcacgtccac ccggccgtgg ttcgcgccgg ccgcggcgat cgcggcgacc
      181 gccgagcgca cccccctcgc cgtcacccag gagcactggc ggcagcacga tcggcagatc
      241 gccatggcgg ccgcgctcga cgactaccgg cgcgatcccg acttcacgcg cgagcgctgg
      301 cagcccgagc agaccatgct gccgccgcag ttcgagaact tcttccagtg gggcatgacg
      361 atcgacacga tgatctgcag cggctgcagc gcgtgcgtga tcgcatgcca ggccgagaac
      421 aacgtgccat cggtcggcgc cgaggacgtc cgccgcaacc gcgagatgca ctggctgcgg
      481 atcgacctct accgcgaggg cagcgaggac gatccgcggt acatcaacca gccgatgctg
      541 tgccagcact gcgcgaacgc gccgtgcgag tacgtgtgcc cggtctacgc cacgcagcac
      601 agccccgacg ggctcaacga gatgatctac aaccgctgca tcggcacgcg gttctgctcc
      661 aacaactgtc cctacaaggt ccggcggttc aactggttca acttcacgcg ggacacgccg
      721 cccacccggc agctgcagta caacccgaac gtcaccgtcc gcgatcgcgg cgtgatggag
      781 aagtgcacgt actgcgtgca gcggatccgc gccgccgaca tcgcggtgcg caagcagcac
      841 cgcgacatcc gccccggcga ggtcgtgacg gcgtgccagc aggcgtgtcc gaccggcgcg
      901 atccagttcg ggcggctcga ccacgaccag acgctcaacg tgcagtggcg cggcgagagc
      961 cgccactatg cggtgctcag ccagctcggg acgcggccgc gcacggtgta cctcgcgaag
     1021 atctggaatc cgagccccga cttgccgatg cagctcggcg agggggatcc ggaacgggat
     1081 ccagaacggg atccggaggg agatcgatga cccagccgct gctgatcggg cgcccgagcg
     1141 acgccgccct cggcgacgag ctgctcggca ccacctggcg cgccggcggc accggctgga
     1201 agatcgcgtt cgcgatcacc ggcgcgctga cggggctcct gatcgcgctc ctcacctacg
     1261 ccatggcgac cgggatcggc gtgttcggga acaacatccc ggtcgcctgg gcgttcccga
     1321 tcatcaactt cgtgtggtgg atcgggatcg gccacgccgg gacgttcatc tccgccatcc
     1381 tgtacctgtt cgagcagggc tggcggacgt cgatcaaccg gctgtccgag gcgatgacgc
     1441 tgttcgcggt gctgcaggcc gcgatcttcc ccgtgctgca cctcggccgg ccgtggttcg
     1501 cgtactggct gtttccgtat ccatcgctga tggaggtctg gccgcaggtc aagagcgcgc
     1561 tgccgtggga cgccgccgcg gtctcgacct acttcacggt gtcgctgctg ttctggtacc
     1621 tcgggctggt cccggacttc gcgtcgctgc gcgacagcgc gccgacccgg cggcgccggg
     1681 cgatctacgg cctgttcgcg ctgggctgga acggcagcgc gaccacctgg cgccactaca
     1741 aggcggtcta cctggtgctg gccgggctcg cgacgccgct ggtgctcagc gtgcacagcg
     1801 tggtgtcgag cgacttcgcg atcgcgctgg tccccggctg gcactcgacg atcttcccgc
     1861 cgtacttcgt cgccggcgcg atcttcagcg gcttcgccat ggtgctcacg ctcgcgatcc
     1921 cgacgcgccg cgtgttcggg ctgcacaacg tcatcaccac caagcacctg gagaactgcg
     1981 cgaagctgat catggtcacc ggctggattg tcgcctacgg ctacatgatg gagtggtttc
     2041 tcgactggta ctcgggcgac ccctccgaga tgtaccaggc gttcgtcgcc cggccgttcg
     2101 gacccaacaa ggtcgtgttc tacctgtcga tgatctgcaa cgtcggcgtg ccgcagatct
     2161 tctggtggaa gcgcgcgcgc ctcaacgtct ggatcctgtg gttcgccgcg ctgctgatca
     2221 acgtcggcat gtggtcggag cgcatgatga tcatcgtcca gagcctgcag cgcgagttcc
     2281 tgccctcgca gtggggtgac tactcgccga cctgggtcga catcggcatc ctgttcggca
     2341 cggtcgggtt cttcacgttc ctgttcctcc tgtttctccg cttcgtcccg ttcatctcgc
     2401 tgtcggagat gcgcgagatg acgcacgagc tcgtcaagca cgagcgcggc gccggcgcgc
     2461 cgcaccccga ggagcacccg catggctgac cggatcctgg cccagtaccc gcacgaggac
     2521 gccctcgtcg cggcgatcca ccggctgcgc gacaagggct atcgcgcgct cgaggcctac
     2581 ctgccgttcc cggcgatcgg cgtcgaggaa gcgctgacca ccccgtcgcg cctgccgtac
     2641 ctgtgcttct tcttcggcat cctggccgcg gcgacggcgt acgcgctgca gtggctgctc
     2701 aacggatacc tctacccgct ggtggtcggc ggccggccgc cgcacttccc gctcacgttc
     2761 gtgccgatca cgttcgagat gggcgtcttg ttcgcgtcgt tcaccgcctt cctcggcctg
     2821 ctgtggcgcg gccggctggt ccggctcacc gacccgatcc agggcacgcc gggcttcgac
     2881 tcggcgaccc gcgaccggtt ctggctcgag gtctcggccg ccgatccggc gttcgatctc
     2941 gaccggaccc gcgccgatct ggtcgagacc ggcgcgctgc gcatcgagct cccggaggtc
     3001 gggccatgat ccgcgcgctg atcaccgtcg cgctgtgcgg gctgaccggc tgcggcgggt
     3061 gctcgccgtg ggatctcgac ctcgagcgca tgatcgagca gccgaagtac acgacctacc
     3121 aggcgtgcga ggtctgcccg ggcggcacga tcatgatgcc cccgccggcc ggcacgatgc
     3181 cgcgcggcga gccggtcgcg ccgacgccgc tggtgctcgg ccgcgaggca ggtcgtaccc
     3241 tcgatcggtt tccggtcgcg gtgacccgcg gcgtgctcga ccgcggccgc aaccggttcg
     3301 acatcttctg tgcggcgtgc cacggccggc tcggcaacgg cgtgtccaag gtcgccgaga
     3361 acatgacatt gcgccggccc gccaacctgt tgaccgcgcc ctatacggaa taccccccgg
     3421 gtcggatctt caacacgatc acccagggct acggcctgat gcggtcgtac gcaggcgagc
     3481 tgccgatcgc cgaccgctgg gcggtggtcg cgtacgtcca ggcgctccag ctgtcgcagc
     3541 acgtcgagct cgacgcgctg ccggcgcacg accgatcgga ggcatcgcga tggctgaagt
     3601 gaggcgcttt gcaggcacgc ggatcgcggg ga
//
LOCUS       DASVOR010000820         2354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_270833,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000820 DASVOR010000000
VERSION     DASVOR010000820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270833"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..>2354)
                     /locus_tag="VF516_16185"
     CDS             complement(28..>2354)
                     /locus_tag="VF516_16185"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl hydrolase family 65 protein"
                     /protein_id="HEX8109273.1"
                     /translation="EPWSVHETALSLDHLAATESLFALSNGHIGVRGNLDEGEPHGLP
                     GTYLNGVYELHPLPYAETNYGAPESGQTIINVTNGKLIRLLVGDEPFDLRYGELLAHD
                     RLLDLRTGTLRRTVKWESPAHRTIRVTSTRFVSFVHRAILAISYEVEPLDGLAEIVVQ
                     SELVANESVPVHAKDPRAAIALESPLEAEHSSWWDSSAILVHRTKRSGLRVAAMMDHT
                     VSGTAQMRTSTEVTPDLARFVIIDALEPGQKLRLVKFVAYGWSEKRTLPALRDQVAAA
                     VSSAKQAGWDDLLAEQRAYLDEFWAHADVELEGDAELQQAIRFALFQVLSAAARAERR
                     GIPAKGLTGNGYDGHTFWDADLYVVPVLTYTVPAAAADALRWRQSILPAAEARAQSLG
                     HAGAAFPWRTIHGEEGSGYWPAGTAAFHINADIAAAVLWYVEATGDERFERDVAVEIL
                     VKTARLWRSLGHHDLQGEFRIDGVTGPDEYSALADDNVYTNLMAQRNLSGAADVCARH
                     VDRALEFGVTPDEMAFWRAAAERMRIPYDHRLGVHPQAEGFTDHEVWDFEHTRPDQYP
                     LLLYFPYFDLYRKQVVKQPDLVLAMVLCPWAFTPDQLDRNFAYYERITVRDSSLSAAA
                     QAIAAARCDHLRLAFDYAAEATLIDLRDLEHNVADGLHLGALAGSWLAFVFGFGGMRV
                     NGGALAFRPKLPEGLTRYSFSITWRARCLHVDVTRARAKYTLRTPGTVRIAHYGEALE
                     VTDTAPISRPLPPIPPREPPTQPPGRAPRRRSPG"
BASE COUNT          337 a          815 c          870 g          332 t
ORIGIN      
        1 gccccgtgca gcgggtcgca cacggcgcta ccccggcgag cggcggcggg gcgcacggcc
       61 gggcggctgg gtcggcggct cgcgcggcgg gatcggcgga agcggacgcg agatcggcgc
      121 cgtgtcggtg acctcgagcg cctcgccgta gtgcgcgatc cgcaccgtgc cgggagtccg
      181 cagcgtgtac ttcgcccgcg cgcgcgtgac gtcgacgtgc aggcaccgcg cgcgccacgt
      241 gatcgagaac gagtaacgcg tgagtccctc gggcagcttg ggccggaacg ccagcgcgcc
      301 gccgttcacc cgcatgccac cgaagccgaa cacgaacgcc agccaggatc cggccagcgc
      361 ccccaggtgc aggccgtcgg cgacgttgtg ctcgagatcg cggagatcga tgagcgtggc
      421 ctcggccgcg tagtcgaagg ccagccgcag gtgatcgcag cgtgcggcgg cgatcgcctg
      481 ggcggccgcg gacagcgacg aatcccggac cgtgatccgc tcgtagtagg cgaagttgcg
      541 gtcgagctgg tcgggcgtga acgcccacgg acagagcacc atcgcgagca cgaggtcggg
      601 ctgcttgacg acctgcttgc ggtacagatc gaagtacggg aagtacagca gcagggggta
      661 ctggtccgga cgcgtgtgct cgaagtccca cacctcgtga tcggtgaagc cctcggcctg
      721 cgggtggacg ccgagccggt ggtcgtaggg gatccgcatg cgctcggcgg ccgcgcgcca
      781 gaacgccatc tcgtcgggcg tgaccccgaa ctccagcgcg cgatcgacgt ggcgcgcgca
      841 cacgtcggcc gcgccgctca ggttgcgctg cgccatcagg ttggtgtaga cgttgtcgtc
      901 ggcgagcgcg ctgtactcgt ccgggccggt gacgccgtcg atccggaact cgccctggag
      961 atcgtggtgg ccgagcgagc gccacagccg cgccgtcttg accaggatct cgaccgcgac
     1021 gtcgcgctcg aaccgctcgt cgccggtcgc ctcgacgtac cacaggaccg cggccgcgat
     1081 gtcggcgttg atgtggaacg ccgcggtgcc ggccggccag tagccggagc cctcctcgcc
     1141 gtggatcgtc cgccacggga acgccgcgcc ggcgtggccc agggactgcg cgcgggcctc
     1201 ggcggcgggc aggatcgact ggcgccagcg cagcgcatcc gcggccgcgg ccggcacggt
     1261 gtacgtgagc accgggacca cgtacaggtc ggcgtcccag aacgtgtggc cgtcgtagcc
     1321 gttgccggtc aggcccttgg ccgggatgcc tcgccgctcg gcgcgcgccg cggccgacag
     1381 gacctggaac agcgcgaacc ggatcgcctg ctggagctcg gcgtcgccct cgagctcgac
     1441 gtcggcgtgc gcccagaact cgtcgaggta cgcgcgctgc tcggccagca ggtcgtccca
     1501 gcccgcctgc ttggccgagc tcaccgcggc cgcgacctga tcgcgcagcg cggggagcgt
     1561 gcgcttctcc gaccagccgt acgcgacgaa cttgaccagg cgcagcttct gtccgggctc
     1621 gagcgcgtcg atgatgacga accgcgccag atcgggagtc acctcggtcg aggtgcgcat
     1681 ctgggcggtg ccgctcaccg tatggtccat catcgccgca acccgcagcc cgcttcgctt
     1741 ggtccggtgg acgaggatcg cggagctgtc ccaccaggac gagtgctcgg cctcgagcgg
     1801 cgactcgagc gcgatggctg cgcgcggatc cttggcgtgg accgggaccg actcgttggc
     1861 gaccagctcc gactgcacga cgatctcggc gaggccgtcg agcggctcga cctcgtacga
     1921 gatcgcgagg atcgcgcggt gcacgaacga cacgaaccgc gtcgaggtca cccggatcgt
     1981 ccggtgcgcg ggcgactccc attttaccgt gcggcgcagc gtgccggtgc gcaggtcgag
     2041 caggcgatcg tgcgccagca gctcgccgta gcgcagatcg aacggctcgt cgccgaccag
     2101 cagccggatc agcttgccgt tggtgacgtt gatgatcgtc tggcccgact cgggcgcgcc
     2161 gtagttggtc tcggcgtacg gcagcgggtg gagctcgtac acgccgttca ggtacgtgcc
     2221 agggagcccg tgcggctcgc cctcgtcgag gttgccgcgc acgccgatgt ggccgttcga
     2281 cagcgcgaac agcgactcgg tggccgccag gtggtcgagg ctcagcgcgg tctcgtgcac
     2341 cgaccacggc tcga
//
LOCUS       DASVOR010000821         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_329511,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000821 DASVOR010000000
VERSION     DASVOR010000821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329511"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VF516_16190"
     CDS             <1..387
                     /locus_tag="VF516_16190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="BtuF-related (seleno)protein"
                     /protein_id="HEX8109274.1"
                     /translation="WIDPLMIGGTWMPELIELAGGQAAGATAGAPAPTITPADLAALA
                     PDVVVVKPCGFSLPRALEERDLIERHVIAACPTRPRVYVTDGNAYFNRPGPRLVESLE
                     IMAACIHPERFDDLQCTHAAVIARLA"
     gene            complement(406..714)
                     /locus_tag="VF516_16195"
     CDS             complement(406..714)
                     /locus_tag="VF516_16195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109275.1"
                     /translation="MRTIALAALLVFGLVSSADAGRRGGGGGGSHSSRSHSGSHSGMT
                     GTGSSSSSHAVRGYTTKRGTYVAPHRQTNPDHTQRNNYSTKGNVNPWTGKDGTRNASH
                     "
     gene            908..>1932
                     /locus_tag="VF516_16200"
     CDS             908..>1932
                     /locus_tag="VF516_16200"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8109276.1"
                     /translation="MTDSSRPPERRFRVGLLGYGLAGRVFHAPLIAACPDLVLASVVT
                     RDPERRAALRAVHPEAEALDAPEQLWERAGSLDLVVVATPNRSHVPLARAALQAGLPV
                     VVDKPLAPSVAEARALIEAARAAGRWLTVFQNRRWDGDFLTVRKLIADGALGTVHRFE
                     SRFERWNPVLRPGWRESRNPADAGGLLFDLGAHLIDQAIQLFGPVRTVYGELDSRRED
                     AVIDDDAFVALTHASGVRSHLWMSKVAAQRAPRFRVLGDRATFVKYGLDGQEPAMAVG
                     VVPGSASWGEEPADRWGFLGEEGTAQPVATVPGAYPRFYEAVAAALRSDAPLPVDPDD
                     AVTALEI"
BASE COUNT          236 a          701 c          697 g          298 t
ORIGIN      
        1 tggatcgatc cgctgatgat cggcggcacc tggatgcccg agctgatcga gctcgccggt
       61 gggcaggcgg ccggggccac cgccggtgcg cccgccccga cgatcacgcc cgccgacctc
      121 gctgcgctcg cgcccgacgt cgtcgtggtc aagccgtgcg gcttctcgct gccgcgcgcg
      181 ctcgaggagc gcgacctgat cgagcgccac gtcatcgccg cctgccccac ccggccgcgg
      241 gtctacgtca ccgacggcaa cgcctacttc aaccgcccgg gcccccggct cgtcgagtca
      301 ctcgagatca tggccgcgtg catccacccc gagcggttcg acgatctcca gtgcacgcac
      361 gccgccgtca tcgcgcgcct cgcctgaggt cgcacccggt cgtcgctagt gcgaggcgtt
      421 cctggtgccg tccttgccgg tccacgggtt gacgttgccc ttggtcgagt agttgttgcg
      481 ctgggtgtga tccgggttgg tctggcggtg cggcgccacg taggtgccgc gcttggtcgt
      541 gtagccgcgc acggcgtggc tgctcgacga cgagccggtg ccggtcatgc cggagtggct
      601 gccggaatgg ctgcgtgaag agtgcgagcc accgcctcca ccgccgcggc ggccggcgtc
      661 cgccgaggac acgagcccga aaaccagcaa cgctgccagc gcgattgtcc gcatgatcct
      721 catcctcccg atccggtcga cagcccgtca tatcacacag tcggatctcg cagaccgtcg
      781 agatcgggta ctcgcggggt aaccaccgtt gtgcagcttc gacacatccc ccggacgggc
      841 gctgcggccg cggtgagatc tgcccatcgc aggagcggcg cgcgtcctgt tgttgtttac
      901 tggtgaaatg acggactcat cgcgcccgcc tgagcgccgg tttcgcgtcg gcttgctcgg
      961 ctacggcctc gccggccggg tgtttcacgc gccgctgatc gcagcgtgcc ccgatctcgt
     1021 gctcgcctcg gtggtcaccc gcgatcccga gcggcgcgcc gcgctgcgcg ccgtgcatcc
     1081 cgaggccgag gcgctcgatg cgcccgagca gctgtgggag cgcgccggct cgctcgacct
     1141 cgtggtggtc gcgacgccca accgcagcca cgtcccgctc gcgcgcgccg cgctgcaggc
     1201 cggcctgccg gtcgtggtcg acaagccgct cgcgccgagc gtggccgagg cccgcgcgct
     1261 gatcgaggcg gcgcgggcgg ccggccggtg gctcaccgtg ttccagaacc ggcgctggga
     1321 cggcgatttc ctgaccgtgc gcaagctgat cgccgacggc gccctgggca cggtccatcg
     1381 cttcgagtcg cggttcgagc gctggaaccc ggtgctgcga ccgggctggc gcgagagccg
     1441 caatccggcg gacgcaggcg ggctgctgtt cgacctcggc gcgcacctga tcgaccaggc
     1501 gatccagctg ttcgggccgg tgcgcacggt ctacggagag ctcgacagtc gccgcgaaga
     1561 cgcggtcatc gatgacgatg cgttcgtcgc gctgacccac gcgtcgggcg tgcgctcgca
     1621 cctgtggatg agcaaggtcg ccgcgcagcg cgcgccgcgg ttccgcgtgc tcggcgaccg
     1681 cgcgacgttc gtcaagtacg ggctcgacgg gcaggagccg gcgatggcgg tcggcgtcgt
     1741 ccccggctcg gcgagctggg gcgaggagcc ggccgatcgc tgggggttcc tcggcgagga
     1801 gggcaccgcg cagccggtcg ccacggtgcc gggcgcctac ccgcggttct acgaggcggt
     1861 ggccgccgcg ctgcgcagcg acgcgccgct gccggtcgat ccggacgacg cggtcaccgc
     1921 gctggagatc at
//
LOCUS       DASVOR010000822         3582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_534190,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000822 DASVOR010000000
VERSION     DASVOR010000822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534190"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..2698)
                     /locus_tag="VF516_16205"
     CDS             complement(5..2698)
                     /locus_tag="VF516_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109277.1"
                     /translation="MAVSVLLATSSAALAWTGPQATLANKNGRTVIQICDQGGACTTS
                     TAFMLAVRNINWTPALYESQVAINYLSSFASGVVPIIHMVLNSTSDPTIDDAVAHLNT
                     LSPRPYVYLRLRADAPTGAFEAMQMVNLQGQSVSDSNNTVTLSPFSYSEAWLQYQEAQ
                     IEHVLTRFDAEYPGRLIGVNIGYEVGHEWFFRPNGVDATGLPSSTGCGTGPDGAPSCA
                     FVPWSNDSGSQAGSIGRHLFYLHDYSPTTQAAFCAWSVLPASLRTGCRAATVVERNNA
                     VPGVPLPGLGLARGVFLDPGDLGSLRAAYYNRFNSEQTVNAITRILARAKQVSGNRLL
                     TSAYYGYLNDLDAALPSSGHTALSSLLASNAIDIVAGPYSYRSPGYGIGSRWLGNPFL
                     PQGVPDSPRLNGKLWVHEDDTRTFFADEPLNTGTPDTAGSLKTVGTLWDSIRILRRNL
                     LSAGLHNTGSWFLDLGAVGWFGKPTDDSDSQALWANLVNAFNGVNKLQLGAPNRFVPQ
                     VAVFTDDRSPNYLAALTPAGESTFTFSTDIAYSLTDALARIGTPVKQYLLSDLLNASL
                     DVSAIKLAVLPNAWNVPANIRQAIDTRLRTPGRTLLFVYAAGYMNGDAAAATGNIGAF
                     TGINVVAGAGTPQLSESFNVNGTTVPGGPAYPLTPWFQISDSSATPLGSYTFAGGTSL
                     ARKTIAVAGGSYTSVLAAAPTLPVAMLRKISEDAGVFHFTGAGDIVDAAGNMMTLHAA
                     TSGNKTISFPQTMPRIFETAIYPSDSLMCNGCSSLASQFFNAGDTRAFRWTTPPFGNF
                     ELISGSSTLEGWTADMDVPDQSISVSVYINGPVGVGTLFASFTASAPRPDLTAAFGIP
                     GNHGFVQTLASCPHGAMLYAYGLDPESNGDGSSFIGAHACP"
     gene            2997..>3582
                     /locus_tag="VF516_16210"
     CDS             2997..>3582
                     /locus_tag="VF516_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX8109278.1"
                     /translation="MAARILAHIEAGTTDTSDGVWCEPVANYRSPERLRAELEVLRRH
                     PRPLCPAAVVPETGRFLTLRVDGRSIVVVRNEDGVVRGFLNACRHRGNEIAEGSGCAK
                     AFVCRYHGWTYDLDGALRHVPHEDGFPGLDRDRHGLVPVAIEERHGIVFLAGPGAPGA
                     RGDDPGELPELIAADQRLIGSDESVLPVNWKLYLE"
BASE COUNT          596 a         1091 c         1303 g          592 t
ORIGIN      
        1 gcggtcaggg gcaggcgtgg gcgccgatga agctcgagcc gtcgccgttg ctctccggat
       61 cgaggccgta tgcatatagc atcgcgccgt gtgggcacga cgcaagtgtc tgcacgaagc
      121 cgtgattccc cgggatgcca aacgccgccg tcaagtccgg ccgcggagcg gacgcggtga
      181 agctggcgaa cagcgtgccg acgcccacgg gaccgttgat gtacacgctc accgagatcg
      241 attgatccgg aacgtccatg tccgcagtcc agccttccag cgtggagcta ccgctgatca
      301 actcgaagtt gccgaacggc ggcgtggtcc agcgaaacgc gcgcgtgtcg ccggcgttga
      361 agaactgcga agcgagcgac gagcaaccat tgcacatcag gctgtcggac ggatagatgg
      421 cggtctcgaa gatccgcggc atggtctgcg gaaagctgat ggtcttgttg ccagaggtcg
      481 cggcgtgcag ggtcatcatg ttgccggctg cgtcgacgat gtctcctgcg ccggtgaagt
      541 ggaacacgcc ggcgtcctcc gagatcttgc gcagcatcgc gaccggcagg gtgggggctg
      601 cggccaggac cgacgtgtac gagccgccgg cgacggcgat ggtcttgcgc gcgagcgagg
      661 tgccgccggc gaacgtgtac gagccgagcg gcgtcgccga cgagtcgctg atctggaacc
      721 acggcgtgag cgggtacgcg ggcccgcccg ggacggtcgt tccgttgacg ttgaagctct
      781 cgctgagctg cggcgtgccc gcgcccgcga ccacgttgat gccggtgaag gcgccgatgt
      841 tgccggtggc ggccgcggcg tcaccgttca tgtagcctgc cgcgtagacg aacaggagcg
      901 tccggcccgg tgtccgcagc cgggtatcga tggcttgccg gatgttggcc ggtacgttcc
      961 aggcgttggg cagcaccgcg agcttgatgg cgctcacgtc caaactggcg ttcagcagat
     1021 cgctgagcag atattgcttc accggcgttc cgatgcgcgc cagtgcgtcg gtcagcgaat
     1081 aggcgatgtc ggtcgagaac gtgaaggtgc tctcgccggc cggggtgagc gcggccaggt
     1141 agttcggtga gcggtcgtcg gtgaacacgg cgacctgcgg cacgaagcgg ttgggcgccc
     1201 cgagctggag cttgttcact ccattgaagg cgttgaccag gttggcccac agcgcctgcg
     1261 agtcggaatc gtcggtgggc tttccgaacc agccgacggc acccaggtcg aggaaccagg
     1321 agccggtgtt gtgcaggccg gcggacagca ggttgcggcg gaggatccgg atcgaatccc
     1381 acagggtacc taccgtcttc aaggatcccg cggtatccgg ggtgcccgtg ttgagtggct
     1441 catcggcaaa gaacgtgcgg gtatcgtcct cgtggaccca cagcttgccg ttcaaacgtg
     1501 gcgagtcggg cacgccctgc ggcaggaagg ggttgccgag ccagcggctg ccgattccat
     1561 agccggggct tcgataggag tacggaccgg cgacgatgtc gatagcgttg ctggcgagca
     1621 gcgacgacag ggcggtgtgg ccgctgctcg gaagcgcggc gtcgagatcg ttcaagtagc
     1681 catagtacgc ggacgtgagc agccggtttc cggacacctg cttggcccgc gccaggatgc
     1741 gggtgatcgc attgacggtc tgctcggagt tgaaccggtt gtaataggcc gcccgcaacg
     1801 aaccgagatc gccgggatcg agaaacacgc cgcgcgcgag gcccagcccg ggcagtggga
     1861 cgcccggcac ggcgttgttg cgctcgacca cggtcgcggc gcggcagccc gtccgcagcg
     1921 aagcgggcag caccgaccag gcgcagaacg cggcctgggt cgtcggcgag tagtcgtgca
     1981 ggtaaaacag gtggcggccg atcgagccgg cctgcgaacc ggagtcgttg gaccacggca
     2041 cgaacgcgca gctgggggcg ccgtcggggc cggtgccgca tccggtgctg ctcggcaggc
     2101 cggtggcgtc gactccgttc ggccggaaga accactcgtg gccgacctcg tagccgatgt
     2161 tcacgccgat gaggcgtccg gggtactctg catcgaagcg ggtgagcaca tgctcgatct
     2221 gcgcctcctg gtactgcagc caggcctcgc tgtacgagaa cggcgagagc gtgacggtgt
     2281 tgttgctgtc gctcaccgac tggccctgca gattgaccat ctgcatcgcc tcgaacgccc
     2341 ccgtgggagc gtctgcccgg agccgcaggt acacgtaggg ccgcgggctc aacgtgttca
     2401 ggtgtgcgac cgcatcatcg atcgtcggat cgctcgtcga gttgagcacc atgtggatga
     2461 tgggcaccac gccgctggcg aacgagctca ggtagttgat ggcgacctga gactcgtaga
     2521 gcgcgggggt ccagttgatg ttgcggaccg ccagcatgaa ggcggtcgag gtggtgcagg
     2581 caccgccctg atcgcagatc tggatcaccg tgcggccatt cttgttggcg agcgtggctt
     2641 gcgggccggt ccaggcgagc gcggcggaag atgtggcgag gaggacagaa actgccatcc
     2701 agatcgcgcg ggaacgtttc atgagacctc ggggagttgc ggaagatgga ctatgtcgaa
     2761 acggggctgt gtcgtcaagg tcgagtcagg tcacgagtct ggatttcaat ccgtccagtc
     2821 gccgggaaga catttctccg aattcacccg agcagtcgag tgagtcgcca tccccggagc
     2881 gcggtctcat gggctcctca tgaaggaacg actcacactg gcacggcgca aacgagcggg
     2941 ccgacgttat catggcgggg aggagatcca tgtccagctt tcgcgatgac cgggccgtgg
     3001 ccgcccgtat cctggcgcac atcgaggcgg ggactaccga caccagcgat ggcgtgtggt
     3061 gcgaaccggt cgcgaactat cgctcgccgg agcggctgcg cgccgaactc gaggtcctgc
     3121 ggcgccatcc ccggccgctg tgcccggcgg cggtggtgcc cgagaccggg cggttcctga
     3181 cgctccgcgt cgacggccga tcgatcgtgg tggtccgcaa tgaggatggc gtggtccgcg
     3241 ggttcctgaa cgcgtgccgg catcgcggca acgagatcgc cgaaggctcg gggtgcgcga
     3301 aggcgttcgt gtgccgctac cacggctgga cctacgatct cgacggcgcg ctccggcacg
     3361 tgccgcacga ggatggcttc cccggcctcg accgcgatcg ccatgggctg gtcccggtcg
     3421 cgatcgagga gcgccacggc atcgtgttcc tcgccggtcc cggcgcgccg ggtgcgcgcg
     3481 gagacgaccc cggcgagctc cccgagctga tcgctgccga tcagcggctg atcgggagcg
     3541 acgagtctgt cttgccggtc aactggaaac tctacctcga ga
//
LOCUS       DASVOR010000823         4806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_241838,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000823 DASVOR010000000
VERSION     DASVOR010000823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4806
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_241838"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF516_16215"
     CDS             complement(<1..218)
                     /locus_tag="VF516_16215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8109279.1"
                     /translation="MELRVNGQPRQVDADPDTPLLWVLRDELGLTGTKYGCGLEQCGA
                     CAVLCDGRAVLSCATPAEAFVGRELRTI"
     gene            324..941
                     /locus_tag="VF516_16220"
     CDS             324..941
                     /locus_tag="VF516_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109280.1"
                     /translation="MPLQNRVTPLGDLIADPARGLVYGNRGCLHDERGEIKRRFATRR
                     WIACRLEFKGWRRKALMQPGKFTELFFLDEATAFAAGHRPCALCRREDYNRFLDLVGA
                     SGADEIDERLHRERLAGAGRRLRPAAPDELPDGAFVLHDGEPWLVLGAHLLRWTPSGY
                     TARVPRSGRRVETITPASLLAVLRAGWEPVVPLLHPSATAPARTR"
     gene            complement(957..3506)
                     /locus_tag="VF516_16225"
     CDS             complement(957..3506)
                     /locus_tag="VF516_16225"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX8109281.1"
                     /translation="MRKVVLWTAFCFFACGGPHASNPDGGSTGGGGGVCGDGKVTDNE
                     QCDDGNTVPGDGCSANCQAEQGFMCIVPNAPCLQIKYCGDGIVQPPEVCDDGNSKGGD
                     GCDGTCHPEPNFTCPTPGQPCVSAFACGDGKVQGTEQCDDGNTTPGDGCEANCTITPG
                     WTCAMPGTPCTKTCGNGKIDPGEQCDDGNTGSGDGCSTSCQIENNFVCPVPGMPCVAA
                     VCGDGKVHGSEQCDDGNLTDNDGCSATCMVEDGWVCPNPGRPCISDCGDGKVRGTEQC
                     DLGPNNGQNMGCSATCTVQPGFVCPGNVCRSTVCGDGVKEGSEQCDDGNLVPYDGCSP
                     TCTIEPKCTGGTCTAVCGDGLKFPNEECDDGNLQDGDGCSHDCKLEPGFNCAANNQPP
                     AATLVIPILYRDMLYNGTKAPPFAAPGHADFQNFNSGIAHGLVKAALGADGKPVWNNN
                     FGDNSGQSLTGAVNFCWWYHDTGCNGATSTNAFARPVFLDAAAKPTTLTLTQISPNVY
                     QFNNQTFFPLDGLGWNAVAGATPQTDLGSDGIRHNFSFTSELHYPFTYDASKPPKFDF
                     TGDDDVWVFINGHLAVDLGGIHAATNGSVTLDAAHATQFGLVDGGMYSIDMFQAERHT
                     LASTYKLTLSGFVHTVTTCSPICGDGVVEGNEVCDDGINDGRPGGCMPGCMARAPFCG
                     DGVVTTPPEACDDGSNLVTYGGTSKLCGPGCQFAPYCGDGVVSNGEQCDDGPKNGMST
                     CSASCTLPAYCGDGIKNGTEQCDNGVNDNSYGTCNRDCTLAPYCGDGSKNGPEQCDLG
                     AMNSPTAYGPGQCTAACTIAPYCGDGIVEPQFGEQCDGGAGCFDCHYAIIQ"
     gene            complement(3798..>4806)
                     /locus_tag="VF516_16230"
     CDS             complement(3798..>4806)
                     /locus_tag="VF516_16230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8109282.1"
                     /translation="AAQLDAALAVVAAPQPGLGETRAVVIIHRGKLVAERYMPGYGPD
                     TPLLSWSMAKSITHAMVGIAVRKGLVDIDKPMGNPRWPAGDARAQIPWRNWLNMIDGQ
                     DYHEIGVTDETRSDAAKMLYGPGRLDVAGYSASLPLVHPPATHWNYNSAGVNLIADAL
                     GRVFAPGAVGAERRARMSATMKAELFAPIGMTSAQPEFDAAGTFVGSAFVYATARDWA
                     RFGLLYLRDGMWQDTRILPEHWVDFARTRTPVADCDTYGAGFWITPPTGRGKPDRALT
                     PDGPRDLFAAQGHEGQLVVIVPSKDLVVVRLGHLADLSGWAPLGAWVERVVQLFPDAR
                     A"
BASE COUNT          664 a         1700 c         1685 g          757 t
ORIGIN      
        1 tcgatcgtgc gcagctcgcg gccgacgaac gcctcggccg gggtcgcgca cgacagcacc
       61 gcgcgcccgt cgcacagcac cgcgcacgcg ccgcactgct cgagcccgca gccgtacttc
      121 gtcccggtga gcccgagctc gtcgcgcagc acccagagca gcggcgtgtc cgggtcggcg
      181 tcgacctggc gtggctggcc gttcactcgc agctccatcg cgggcgatgc tagcaccgct
      241 ccgagcccgc ggtgccggcg cctgcacgac atccgggagg cggagaatcg ctcatgggcg
      301 tactccggcg atactgtcga gcgatgccgc tccagaaccg cgtcaccccg ctcggcgacc
      361 tgatcgccga ccccgcccgc gggctcgtct acggcaaccg cggctgcctc cacgacgagc
      421 gcggcgagat caagcgccgg ttcgccaccc ggcgctggat cgcctgccgg ctcgagttca
      481 agggatggcg gcgcaaggcg ctgatgcagc cgggcaagtt caccgagctg ttcttcctgg
      541 acgaggcgac cgcctttgcc gccgggcacc gcccctgcgc gctgtgccgc cgcgaggact
      601 acaaccggtt cctcgatctc gtcggtgcga gcggcgccga cgagatcgac gagcggctcc
      661 accgcgagcg gctcgccggc gccggccggc gcctccgccc cgccgcaccc gacgagctcc
      721 cggacggagc gttcgtgctc cacgacggcg agccgtggct cgtgctcggc gcccacctcc
      781 tgcgctggac gccgtccggc tacaccgcgc gcgtcccccg atccggacgc agggtggaga
      841 cgatcacgcc ggcgtcgctg ctcgcggtgc tgcgcgccgg ctgggagccg gtcgtgccac
      901 tcctgcaccc atcggcgacg gcgccggctc gaacgcggtg acggtcgcgg tcccccctac
      961 tggatgatcg cgtagtggca gtcgaagcag ccggcgccgc catcgcactg ctcgccgaac
     1021 tgcggctcga cgatgccgtc gccgcagtac ggcgcgatgg tgcacgccgc ggtgcactgc
     1081 cccgggccgt aggcggtggg agagttcatc gcgccgaggt cgcactgctc ggggccgttc
     1141 ttgctgccat cgccgcagta cggcgccagc gtgcagtcgc ggttgcaggt gccgtaggag
     1201 ttgtcgttga cgccgttgtc gcactgctcg gtgccgttct tgatgccgtc gccgcagtac
     1261 gcggggagcg tgcacgacgc cgagcaggtc gacatcccgt tcttcgggcc gtcatcgcac
     1321 tgctcgccgt tgctgaccac gccgtcgccg cagtacggcg cgaactggca gcccggaccg
     1381 cacagcttgc tggtcccgcc gtaggtcacg aggttgctgc cgtcatcgca cgcctcgggc
     1441 ggcgtggtca cgacgccgtc gccgcagaac ggcgcgcggg ccatgcagcc gggcatgcag
     1501 ccgccgggtc tgccgtcatt gatgccgtcg tcgcacacct cgttgccctc gacgacgccg
     1561 tcgccgcaga tcgggctgca cgtggtcacg gtgtggacga acccggacag cgtcagcttg
     1621 taggtcgacg cgagggtgtg gcgctcggcc tggaacatgt cgatcgagta catcccgccg
     1681 tcgaccaggc cgaactgcgt ggcgtgcgcc gcgtcgagcg tgacgctgcc gttggtcgcc
     1741 gcgtggatgc cgccgaggtc gaccgcgagg tggccgttga tgaacaccca gacgtcgtcg
     1801 tcgccggtga agtcgaactt cggcggcttg ctcgcgtcgt aggtgaacgg atagtgcagc
     1861 tcgctggtga acgagaagtt gtgccggatg ccgtcgctgc cgaggtcggt ctgcggagtg
     1921 gccccggcga cggcgttcca gcccagtcca tcgagcggga agaacgtctg gttgttgaac
     1981 tggtagacgt tgggcgagat ctgggtcagc gtcagcgtcg tcggcttggc cgcggcgtcg
     2041 aggaacaccg gccgggcgaa ggcgttggtc gaggtcgcac cattgcagcc ggtgtcgtgg
     2101 taccaccagc agaagttgac cgcgccggtc agcgactgcc ctgagttgtc gccgaagttg
     2161 ttgttccaca ccggcttgcc atccgcgccg agcgcggctt tcaccagacc gtgggcgatc
     2221 ccgctgttga agttctggaa gtctgcgtgg ccgggggccg caaacggcgg cgccttggtc
     2281 ccgttgtaaa gcatgtcgcg atacaggatc ggaatgacca gcgtggccgc cggcggctgg
     2341 ttgttcgccg cgcagttgaa gccgggctcc agcttgcagt cgtgggagca gccgtcgccg
     2401 tcctggaggt tgccgtcgtc gcactcctcg ttcgggaact tgaggccgtc gccgcacacc
     2461 gccgtgcagg tgccgccggt gcacttgggc tcgatggtgc aggtcggcga gcaaccgtcg
     2521 taggggacca ggttgccgtc gtcgcactgc tcgctgccct ccttcacgcc gtcgccgcac
     2581 acggtcgaac gacacacatt gccgggacac acgaagccgg gctggaccgt gcacgtggcg
     2641 gagcagccca tgttctgtcc gttgttcggg cccaggtcgc actgctcggt gccgcggacc
     2701 ttgccgtcgc cgcagtccga gatgcagggc cggccagggt tcgggcacac ccagccgtcc
     2761 tcgaccatgc aggtcgccga gcaaccgtcg ttgtccgtca ggttgccgtc gtcgcactgc
     2821 tcgctgccgt ggaccttgcc gtcgccgcac accgcggcca cgcacggcat gccggggacc
     2881 gggcacacga agttgttctc gatctggcag ctggtgctgc agccgtcgcc gctccccgtg
     2941 ttgccgtcgt cgcactgctc gcccggatcg atcttgccgt tgccgcacgt cttcgtgcag
     3001 ggcgttccgg gcatcgcaca ggtccagccc ggcgtgatcg tgcagttggc ctcgcagcca
     3061 tccccgggcg tggtgttgcc gtcgtcgcac tgctcggtgc cctggacctt gccgtcgccg
     3121 cacgcgaacg ccgacacgca cggctgcccc ggcgtcggac aggtgaagtt cggctcgggg
     3181 tgacaggtgc cgtcgcagcc gtcgccgccc ttcgagttgc catcgtcgca gacctcgggc
     3241 ggctggacga tgccgtcgcc gcagtacttg atctggaggc acggagcgtt cgggacgatg
     3301 cacatgaagc cctgctcggc ctgacagttg gcgctgcaac cgtcgcccgg gaccgtgttg
     3361 ccgtcgtcgc actgctcgtt gtcggtgacc ttgccgtcgc cgcagacccc gccgccaccg
     3421 ccggtcgagc cgccgtcggg gttgctggcg tgaggtccac cgcacgcgaa gaaacagaat
     3481 gctgtccaca gaacgacctt gcgcatcacc accaggctac ccgccgcgag aaaagactca
     3541 atgctttctg cggcagctct tctcgacgtg tcggatcgcc acatcggtag gctggagtgc
     3601 gacgcacgcg cgtacgtgct cgccgcatgc aagtccgtgg tctccttggc gccggcgcaa
     3661 gagccgtcgg cgatcgggac aactgtcccc gggccagccg accgcagcga cgatcgggac
     3721 aactgtcccc ggtgatcgga cagcaagagc gaccggcagc gcccccgctc ggcgacggtg
     3781 gcctggccgg cggtcgacta cgcgcgagcg tcggggaaca gctgcaccac gcgctcgacc
     3841 cacgcgccga gcggcgccca gccggacaga tcggcgaggt ggccgaggcg gacgaccacc
     3901 agatccttcg acggcacgat caccacgagc tgaccctcgt ggccctgcgc ggcgaacaga
     3961 tcgcgcggac cgtcgggggt gagcgcccgg tccggcttgc cccggccggt gggcggcgtg
     4021 atccagaagc cggcgccgta ggtatcgcag tccgcgacgg gggtcctggt gcgcgcgaag
     4081 tcgacccagt gctcgggcag gatgcgtgtg tcctgccaca tcccgtcgcg caggtagagc
     4141 agaccgaacc gcgcccagtc gcgcgccgtc gcgtacacga acgccgagcc gacgaaggtg
     4201 ccggccgcgt cgaactcggg ctgcgccgac gtcatgccga tcggcgcgaa cagctcggcc
     4261 ttcatcgtcg cgctcatccg ggcgcgacgc tccgcgccca cggccccagg tgcgaacacc
     4321 cggcccaggg cgtcggcgat caggttcacg cccgccgagt tgtagttcca gtgcgtcgcg
     4381 ggcgggtgga cgagcggcag cgacgccgag tagccggcga catcgagccg gccggggccg
     4441 tacagcatct tggcggcgtc gctgcgcgtt tcatcggtca cgccgatctc gtgatagtcc
     4501 tgcccgtcga tcatgttgag ccagttgcgc cagggaatct gcgcgcgcgc gtcgccggcg
     4561 ggccagcgcg gattgcccat cggcttgtcg atgtcgacca ggcccttgcg caccgcgatg
     4621 ccgaccatcg cgtgcgtgat cgacttggcc atcgaccacg acagcagcgg cgtgtcgggg
     4681 ccgtagccgg gcatgtagcg ctccgcgacc agcttgccgc ggtggatgat gaccaccgcg
     4741 cgggtctcgc cgaggcccgg ctgcggcgcg gcgaccacgg ccagcgccgc gtcgagctgc
     4801 gcggcg
//
LOCUS       DASVOR010000824         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_475870,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000824 DASVOR010000000
VERSION     DASVOR010000824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475870"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2092
                     /locus_tag="VF516_16235"
     CDS             <1..>2092
                     /locus_tag="VF516_16235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109283.1"
                     /translation="IGIHESEPLSAQHDQLRARVAERVPAGDRHRVTAFLGELVGAAV
                     SNDSGDDDPALRAARQDARLMNEQMRRAWLDFLLAETAAHPVLVVLDDLHWGDFGTVR
                     FLDAALRDRGKQPWMVLALARPEVFEVFPRLWAERQNVQALRLKELGRKAGERLVRQV
                     LGDAVGSDTVERLVKQADGNAFYLEELIRAVAEGKDAALPETVLAMVETRLARLPFEA
                     RRVLRAASVFGEVCWESGVVTLLGGAMGATMVHTWLAKLVEQELLIARPDSRFPGERE
                     LAFRHALLREGAYATLTADDQRRAHRLAGEWLERSGEAEPMVLAGHARRGGDLARAAA
                     LYLRAAEQAVHVLDNEGAIARASLGLGCDPLPELRFALLGIRCHASHRLQRHLQGHGL
                     ADAEELVRSAPPGSVPWAQGMNSYQLAMMMTGREDDLRASLASLAEVTPAPGAAGWIS
                     FLFVGSLFLLDHVGRIPPATALEEPFLALARQRRDEEPLAWFWWNVSVGLRASHAHED
                     PWRGLVHCDAIQPISDLIGGDLIFPAMQVLRGMNLWHLGAHARAVELLESVPAADTTL
                     GEISPLRRIVLSWLYADRGALDEARALAIEVREVCREHHDRLDEARCTWVLAEVLRRG
                     GDLEGAEREIEAARAMAMPLDHPSMLATLAVIRLAQGRAADALAAAEDAMARCAAMGG
                     AGLFRAAAVRLVHAE"
BASE COUNT          259 a          760 c          784 g          289 t
ORIGIN      
        1 catcgggatc cacgagagcg agccgctctc cgcgcagcac gaccagctgc gcgcgcgggt
       61 cgccgagcgc gtccccgccg gcgatcgcca ccgcgtgacc gcgttcctcg gcgagctggt
      121 cggcgcggcg gtctccaatg actccggcga cgacgacccc gcgctgcgcg ccgctcgcca
      181 ggacgcccgg ctgatgaacg agcagatgcg cagggcctgg ctcgacttcc tgctggccga
      241 gaccgccgcg cacccggtgc tggtcgtgct cgacgatctg cactggggcg acttcgggac
      301 cgtgcggttc ctcgacgccg cgctgcgcga tcgcggcaag cagccgtgga tggtgctcgc
      361 gctggcgcgc cccgaggtgt tcgaggtgtt ccccaggctg tgggcggagc gtcagaacgt
      421 ccaggcgctc cggctcaagg agctcggccg caaggcgggc gagcggctgg tccgccaggt
      481 gctgggcgac gcggtcggca gcgacacggt ggagcgcctg gtcaagcagg ccgacggcaa
      541 cgcgttctac ctcgaggagc tgatccgcgc ggtcgccgag ggcaaggatg cggcgctgcc
      601 ggagaccgtg ctcgccatgg tcgagacccg gctggcgagg ctgccgttcg aggcccgccg
      661 ggtgctgcgc gccgccagcg tgttcggcga ggtgtgctgg gagagcggcg tggtcacgct
      721 gctcggcggg gcgatggggg cgacgatggt gcacacgtgg ctggcaaagc tcgtcgagca
      781 ggagctgctg atcgcgcggc cggacagccg gttccccggc gagcgcgagc tcgcgttccg
      841 ccacgcgctg ctccgcgagg gcgcctacgc cacgctgacc gccgacgacc agcggcgcgc
      901 gcatcgcctc gccggcgaat ggctcgagcg atccggcgag gccgagccca tggtcctggc
      961 cggacacgcc cggcgcggtg gagacctcgc gcgcgccgcc gcgctctatc tccgggccgc
     1021 ggagcaggcg gtgcacgtgc tcgacaacga aggcgcgatc gcccgcgcca gcctcggtct
     1081 gggctgcgat ccgctgcccg agctccggtt cgccctgctc ggcatccgct gccacgcgtc
     1141 ccaccgcctg caacggcacc tgcaagggca cgggctcgcc gacgcggagg agctggtgcg
     1201 ctcggcgccg ccgggctcgg tcccgtgggc gcagggtatg aactcgtacc agctggccat
     1261 gatgatgacc ggccgggaag acgatctccg ggcgtcgctt gcgtcgctcg ccgaggtcac
     1321 cccggccccc ggagcggcgg ggtggatctc gttcctgttc gtcggcagcc tcttcctgct
     1381 cgaccacgtc gggcgcatcc cgccggccac cgcgctggag gagccgttcc tcgcgctcgc
     1441 ccgccagcgg agagacgagg agccgctcgc gtggttctgg tggaacgtct ccgtcggcct
     1501 gcgggcatcc catgcccacg aggacccatg gcgcggcctg gtgcactgcg atgcgatcca
     1561 gccgatctcc gatctgatcg gtggcgacct catctttccc gccatgcagg tgctgcgcgg
     1621 catgaacctg tggcatctgg gcgcgcacgc tcgcgcggtg gagctgctcg aatccgtccc
     1681 cgcggcagac accacgctgg gcgagatcag cccgctgcgc cggatcgtcc tgtcctggct
     1741 gtacgccgac cgcggcgcgc tcgacgaggc gcgcgcgctg gcgatcgagg tccgcgaggt
     1801 ctgccgcgag catcacgatc gcctggacga ggcccgatgc acctgggtgc tcgccgaggt
     1861 gcttcgccgg gggggcgacc tcgagggcgc cgagcgcgag atcgaggccg cgcgcgccat
     1921 ggcgatgccg ctcgaccatc cgagcatgct ggccacgctg gcggtgatcc gcctcgccca
     1981 gggacgcgcc gccgacgcac tcgcggccgc cgaggatgcg atggcccggt gcgccgccat
     2041 gggcggggcc ggcctgttcc gcgccgcagc ggtgcgcctg gtccacgccg ag
//
LOCUS       DASVOR010000825         3078 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_329571,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000825 DASVOR010000000
VERSION     DASVOR010000825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329571"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VF516_16240"
     CDS             <1..468
                     /locus_tag="VF516_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEX8109284.1"
                     /translation="EFAEVAVDEATGSVTLRATFPNPDGVLLPGMYVRAVLDQAVATT
                     AILAPQQGISHDPKGNATALVVGAGDKVEPRTLVADRAIGDRWLVTSGLSAGDRLVVE
                     GLNKVRPGMLVHPVEVAAAKAAAPAGKPTDAAKPTEAATDAPARPADAPAKER"
     gene            470..>3078
                     /locus_tag="VF516_16245"
     CDS             470..>3078
                     /locus_tag="VF516_16245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8109285.1"
                     /translation="MLARFFANRPVFAWVITISVILAGIASIRALPIAAYPDVALPSV
                     NISAGYPGASAETVENSVTQVLEQQLTGIDGLVYFSSSSSSAGQAGINVTFKQGTNPD
                     TAQVQVQNKVQQAIPRLPPAVQQQGIVVSKAQSNFLMVVIVYDRNDKATEADISDYVT
                     STIQDPIARVDGVGSVQIFGGSYAMRIWIDPTRLQAFSLMPSDVEAAIATQNVQVSAG
                     KIGQQPAPPGQQLNATVTAQSKLRTPQEFRDIILKYDPSGATVRLGDVARVELGTEAY
                     DFAVRLNRHPASGFAVNLAPNANAMAVASAVKDTVSRLEATMPAGWKVAYPFDTTKFI
                     RLSILEVVKTLIEAIALVVIVMFVFLQSWRATLIPVIAVPVVLLGTFSILQLLGYTIN
                     TLTMFAMVLSIGLLVDDAIVVVENTERLMREEHLSPLDATVRSMKEITGALIGIATVL
                     SAVFLPMAFFSGSTGVIYRQFSVTIVASMLLSVLVALTISPAMCASLLRSKGAHGTSH
                     RGPFGWFNRGLERVARGYQGGVARMLRGPFRWLVPYLAIVGAMAWLVLRLPTGFLPTE
                     DQGIGFAIWTLPSGAVLPRTVEVGKAVEHHFLDVEQANVESIATVSGFSFVGVGQNTG
                     IAFIEFKDWDQRPGKASTTEAVTGRAIGMLSAVRDAQIFALTPPAIQGLGNSQGFDFQ
                     LQAAGIDRAALAQARDQLLAAAAKDPKLIAVRRGDLPETPQLHIDIDQAKAVAHGVAP
                     PDIASTLAAAWGGVYVNDFIDRGRVKRVFVQGDAAFRSKPEDLDAWYVRGGSGAMTPF
                     SAFATTRWTFGAESLSRYNGLASYDIQGTGAPGVSSGEAMDEVAGLGRTISGTTSSWS
                     GLSYQEQLASGQPL"
BASE COUNT          452 a         1107 c         1073 g          446 t
ORIGIN      
        1 gagttcgccg aggtcgcggt cgacgaggcc accggcagcg tcacgctgcg cgcgacgttc
       61 cccaaccccg acggcgtgct gctgcccggc atgtacgtcc gcgcggtgct cgatcaggcg
      121 gtcgcgacca cggcgatcct cgcgccgcag cagggcatct cgcacgaccc caagggcaac
      181 gcgaccgcgc tggtggtcgg cgcgggcgac aaggtcgagc cccggacgct ggtcgccgac
      241 cgcgcgatcg gcgatcgctg gctggtgacc agcggcctgt ccgccggcga ccggctggtc
      301 gtcgaggggc tcaacaaggt ccgccccggg atgctggtgc acccggtcga ggtcgcggcg
      361 gccaaagccg cggcgccggc gggcaagccg accgacgcgg ccaagcccac cgaggcggct
      421 acggacgcgc cggccaggcc cgcggacgcg ccggccaagg agcgctgacg tgctcgcccg
      481 gttcttcgcc aaccggccgg tgttcgcgtg ggtgatcacg atttccgtga tcctcgccgg
      541 catcgcctcg atccgcgcgc tgccgatcgc ggcgtacccc gacgtcgcgc tgccgagcgt
      601 caacatctcc gccggctacc ccggcgcgtc ggccgagacc gtcgagaaca gcgtgaccca
      661 ggtgctcgag cagcagctga ccgggatcga cggcctggtc tacttctcca gctcgtcgag
      721 ctcggccggc caggccggca tcaacgtcac gttcaagcag ggcaccaacc ccgacaccgc
      781 gcaggtccag gtccagaaca aggtccagca ggcgatcccg cgcctgccgc cggcggtgca
      841 gcagcagggc atcgtggtgt ccaaggccca gagcaacttc ctgatggtcg tgatcgtcta
      901 cgaccgcaac gacaaggcga ccgaggccga catctcggac tacgtcacca gcacgatcca
      961 ggatccgatc gcgcgggtcg acggcgtcgg ctccgtccag atcttcggcg gcagctacgc
     1021 gatgcggatc tggatcgacc cgaccaggct gcaggcgttc agcctgatgc cgtcggacgt
     1081 cgaggccgcg atcgcgaccc agaacgtcca ggtctcggcc ggcaagatcg gccagcagcc
     1141 ggcgccgccc ggccagcagc tcaacgccac cgtgaccgcg cagtccaagc tgcgcacgcc
     1201 gcaggagttc cgcgacatca tcctcaagta cgacccgagc ggcgccacgg tccggctcgg
     1261 cgacgtcgcg cgcgtcgagc tcggcaccga ggcctacgac ttcgcggtcc ggctcaaccg
     1321 ccaccccgcg tcgggcttcg cggtcaacct cgcgcccaac gccaacgcga tggcggtggc
     1381 gagcgcggtc aaggacacgg tgagccggct cgaggccacg atgccggcgg gctggaaggt
     1441 cgcgtacccg ttcgacacca ccaagttcat ccggctgtcg atcctcgagg tcgtcaagac
     1501 gctgatcgag gcgatcgccc tggtcgtgat cgtgatgttc gtgttcctgc agagctggcg
     1561 ggcgaccctg atcccggtga tcgcggtgcc ggtcgtgctg ctcggcacgt tctcgatcct
     1621 ccagctgctc ggctacacga tcaacacgct gacgatgttc gcgatggtgc tgtcgatcgg
     1681 cctgctggtc gacgacgcga tcgtcgtggt cgagaacacc gagcggctga tgcgcgagga
     1741 gcacctgtcg ccgctcgacg ccaccgtgcg ctcgatgaag gagatcaccg gcgcgctgat
     1801 cggcatcgcg accgtgctgt cggcggtgtt cctgccgatg gcgttcttca gcggctcgac
     1861 cggcgtgatc taccgccagt tctcggtcac gatcgtcgcg tcgatgctgt tgtcggtgct
     1921 ggtcgcgctc acgatctcgc cggcgatgtg cgcgtcgctg ttgcgcagca agggcgccca
     1981 cggcacgagc catcgcgggc cgttcggctg gttcaaccgc ggcctcgagc gcgtggcgcg
     2041 cggctaccag ggcggcgtcg cgcggatgct gcgcggcccg ttccgctggc tcgtgcccta
     2101 cctcgcgatc gtcggcgcga tggcgtggct ggtgctccgg ctgccgaccg ggttcctgcc
     2161 gaccgaggac cagggcatcg ggttcgcgat ctggaccctg ccgtcgggcg cggtgctgcc
     2221 gcgcaccgtc gaggtcggca aggcggtcga gcaccacttc ctcgacgtcg agcaggccaa
     2281 cgtcgagtcg atcgcgacgg tctcggggtt cagcttcgtc ggcgtcggcc agaacaccgg
     2341 catcgcgttc atcgagttca aggactggga ccagcgcccg ggcaaggcca gcaccaccga
     2401 ggcggtcacc ggccgtgcga tcggcatgct gtccgcggtg cgcgacgccc agatcttcgc
     2461 gttgaccccg ccggcgatcc agggcctggg caactcgcag ggcttcgact tccagctcca
     2521 ggccgccggc atcgaccgcg ccgcgctcgc gcaggcccgg gaccagctgc tcgcggccgc
     2581 ggccaaggac ccaaagctga tcgcggtccg gcgcggcgat ctgcccgaga ccccgcagct
     2641 ccatatcgac atcgaccagg ccaaggcggt cgcgcacggc gtggcgccgc ccgacatcgc
     2701 cagcaccctg gcggcggcgt ggggcggcgt gtacgtcaac gacttcatcg atcgcggccg
     2761 ggtcaagcgc gtgttcgtcc agggcgacgc ggcgttccgc agcaagcccg aggacctcga
     2821 cgcgtggtac gtgcgcggcg ggtcgggcgc gatgacgccg ttcagcgcgt tcgcgaccac
     2881 gcggtggacg ttcggcgccg agtcgctgtc gcgctacaac ggcctggcgt cgtacgacat
     2941 ccagggcacg ggcgcacccg gcgtcagctc cggcgaggcg atggacgagg tcgccggcct
     3001 cggccggacg atctccggaa cgacgtcgtc gtggagcgga ctgtcctacc aggagcagct
     3061 cgcgagcggc cagcccct
//
LOCUS       DASVOR010000826         5589 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_66474,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000826 DASVOR010000000
VERSION     DASVOR010000826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5589
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66474"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VF516_16250"
     CDS             <1..469
                     /locus_tag="VF516_16250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109286.1"
                     /translation="AAEPQAPARPVPVPSGPRGPVIRSIPARWRCHKTPGCFSDPWPG
                     SVIVWPAWAAYQDNGRTGNVLRFVYSTKGEPLYPYMGPWAHGCEVTAESGMVNVVEWK
                     RGSDQWRETVLGPGESHVIALVPPEDGALIEGADDSSLFSVSLRGCTPQRIER"
     gene            472..1638
                     /locus_tag="VF516_16255"
     CDS             472..1638
                     /locus_tag="VF516_16255"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8109287.1"
                     /translation="MDFALTEGQQAIVDSVRQLCAGFDLDYWRRRDAEGGFPHDFYAA
                     VARAGYLGIAMPAQHGGAGLGITEAVLVMQAIAQSGAGLSGASAVHMNIFGLHPVVVF
                     GTAEQQARWLPPVIRGEHQACFGVTEPDAGLNTLALKTRAVRDGDDYVVSGQKIWIST
                     AQVAHKMLLLARTTPIAERAAPTDGLTLFYTDLDRSRVEVREIDKHGRACVDSNLVFI
                     DGWRVPAADRIGDEGKGFQYILHGFNPERMLIAAEAVGVGRAALQLAARYASERVVFD
                     RPIGKNQAIQHPLAQRWMELEAANLMILKAAWLYDQGQPCAAEANAAKYLAGEAGYRA
                     CETAVMTHGGMGYAKEYHVERLLRESLIPRIAPVSRELILSFIAEKVLALPKSY"
     gene            complement(1770..2702)
                     /locus_tag="VF516_16260"
     CDS             complement(1770..2702)
                     /locus_tag="VF516_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX8109288.1"
                     /translation="MRRHGILIVEDDYLLAAELGATIQRAGNLLVGPAPSIDQALRLL
                     QDGAVVDAAILDIHLRDEMVFPVADALTARGAPFLFITGHDSVVPERFIQAALCKKPL
                     VPKQVRFELQRVLDPPWFVAIGASGADGLVDLCALLGALPAGLNAAFLVVLHRGWGVS
                     SSLCKILARAGPHRVVVARPGIRLEPGRVYIGEPSRHLVLGRRSDSEVIDDPDRIHGG
                     RTVDLLFHSVAGCAGARTIGVVLSGGLDDGSRGLAAIHQAGGRAMVVQAHSELGSGMP
                     THAMARVSGVNLVGTPSELAREIALITRGLAWPH"
     gene            complement(2711..3025)
                     /locus_tag="VF516_16265"
     CDS             complement(2711..3025)
                     /locus_tag="VF516_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109289.1"
                     /translation="MTDDYRLVAHRGIQTGHLATPERVLVAELQHRTRNLLGVVRAIA
                     SKTPGELRLGRGVRTAVSRSNRRQGCRQGGSRMTTLAGRRIRIYDKPVEPTALAGALL
                     TP"
     gene            complement(3301..4125)
                     /locus_tag="VF516_16270"
     CDS             complement(3301..4125)
                     /locus_tag="VF516_16270"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8109290.1"
                     /translation="MSVTTTRIALATGAQLPQVGLGVYLSPAGAATRDAVTAALELGY
                     RHVDTARIYGNEADVGAAIRAGGVPRDQVFVTTKLWNDDHGYDDALRALDKSLARLGM
                     DHVDLYLIHWPVPGKRRDSWRALERAFSDGKARAIGVSNYLVPHLRELLGEAKVIPHV
                     NQIELSPFLQRRDTCALCREHGIAIEAYSPLTRGQRLGHPAVAAIARATGRTPAQVLL
                     RWGIQHGFVVLPKSVHRERIAENAAVFDFALDAAAMARLDELEEGLATGWDPDGEP"
     gene            4184..4804
                     /gene="lepB"
                     /locus_tag="VF516_16275"
     CDS             4184..4804
                     /gene="lepB"
                     /locus_tag="VF516_16275"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8109291.1"
                     /translation="MSSSPPPAGSRIRRIAREVVMVAVFALVLMSARSSLADHYLVPT
                     GSMQPTVEIDDRVLVNKAAYGLRVPFSHSYLARFDGPAAGDVVVLDAPDEDKVLLKRV
                     VGVPGTRVEVRGGRIRIDGKPAPVAQRGDELFEQLGRADHALRLTRGGGPDWGPVTIP
                     EGRYLVMGDNRGDSRDGRFFGLVTREAILGRAVGVFWRSGPTWDGL"
     gene            4866..5378
                     /locus_tag="VF516_16280"
     CDS             4866..5378
                     /locus_tag="VF516_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109292.1"
                     /translation="MRASRAVGGLRGLALAALAACGGPGVIHPRPPDCRIEVFPSPPG
                     DAYVQVGEMDFGEYVIHKPQYQYKSPYTLAADMRPQICAVGGDTLVAEQDATGVIVRA
                     TVYRHANESDIPAPYPKQPPPKAESCEGGCGPGFACERDVCVPQAPTCDPACGDGETC
                     GADRLCHPDS"
BASE COUNT          809 a         1916 c         2098 g          766 t
ORIGIN      
        1 cgccgccgag ccgcaggcgc cggcgcgccc ggtgcccgtg ccgagcggac ctcgcggccc
       61 ggtgatccgc tcgatcccgg cgcgctggcg ctgccacaag acgcccggtt gcttcagcga
      121 tccgtggccg ggctcggtca tcgtctggcc ggcgtgggcg gcgtaccagg acaacggccg
      181 cacgggcaac gtgctgcgct tcgtgtactc gaccaagggc gagccgctgt atccgtacat
      241 ggggccgtgg gcgcacggct gcgaggtcac ggcggagtcg gggatggtca atgtcgtcga
      301 gtggaagcgc ggatcggacc agtggcggga gaccgtgctc gggccgggcg agtcgcacgt
      361 gatcgcgctc gtcccgccgg aggacggggc gctgatcgag ggggccgacg actcgtcgct
      421 gttcagcgtg tcgctgcggg gctgcacgcc gcagcggatc gagcgctagc catggacttc
      481 gcgctcaccg aaggccagca ggcgatcgtc gacagcgtcc ggcagctgtg cgcgggcttc
      541 gatctggact actggcgccg gcgcgacgcc gagggcggct tcccgcacga cttctacgcc
      601 gcggtcgcgc gggcggggta cctcgggatc gcgatgccgg cgcagcacgg cggcgccggg
      661 ctgggcatca ccgaggcggt gctggtgatg caggcgatcg cgcagtccgg cgcggggctg
      721 tcgggcgcct cggcggtgca catgaacatc ttcgggctgc acccggtggt ggtgttcggc
      781 acggcggagc agcaggcgcg ctggctgccg ccggtgatcc gcggcgagca ccaggcgtgc
      841 ttcggggtca ccgagccgga cgccgggctc aacacgctgg cgctgaagac ccgggcggtg
      901 cgcgacggcg acgactacgt ggtcagcggg cagaagatct ggatctcgac ggcgcaggtc
      961 gcgcacaaga tgctgctcct ggcgcgcacg acgccgatcg cggagcgcgc ggcgccgacc
     1021 gacgggctga ccctgttcta caccgacctc gatcgcagcc gcgtcgaggt ccgcgagatc
     1081 gacaagcacg gccgcgcgtg cgtcgactcg aacctggtgt tcatcgacgg ctggcgggtc
     1141 ccggccgccg atcggatcgg cgacgagggc aagggcttcc agtacatcct gcacggcttc
     1201 aaccccgagc gcatgctgat cgcggccgag gcggtcgggg tcgggcgcgc ggcgctgcag
     1261 ctcgcggcgc ggtacgcgag cgagcgcgtg gtgttcgacc ggccgatcgg caagaaccag
     1321 gcgatccagc acccgctcgc gcagcgctgg atggagctcg aggcggcgaa cctgatgatc
     1381 ctgaaggcgg cgtggctcta cgaccagggc cagccgtgcg ccgcggaggc caacgcggcg
     1441 aagtacctgg cgggcgaggc ggggtatcgc gcgtgcgaga ccgcggtgat gacccacggc
     1501 ggcatggggt acgcgaagga gtaccacgtc gagcgcctgc tgcgcgaaag cctgatcccg
     1561 cggatcgcgc cggtgagccg ggagctgatc ctgagcttca tcgccgagaa ggtgctggcg
     1621 ctgccgaagt cgtactgagc ggtcacggct cgccgtcggg atcccagccg gtggcgagga
     1681 cttcctcgag cccgtcgagc cgtgccatcg gtcgcccatc gtgcaccatc cgcacctggg
     1741 tctgccggta ctcgcagccg gcaagagggt cagtgcggcc atgcgagccc cctggtgatc
     1801 agggcgattt cacgtgcgag ctcgctcggg gttcccacga ggttcacgcc ggacacccgg
     1861 gccatcgcat gggtcggcat cccgctgccc agctcggagt gcgcctggac gaccatggct
     1921 ctgccccctg cctggtggat cgccgcgagc ccgcgcgagc cgtcgtcgag gccgcccgac
     1981 aggaccacac cgatcgttcg cgccccggcg caacccgcga cagaatgaaa caggaggtcg
     2041 acggtgcggc cgccatggat gcggtccgga tcgtcgatca cctcgctgtc cgaccggcgg
     2101 ccgagcacga ggtgccgcga tggctcgccg atgtacacgc gaccgggttc caggcgtatc
     2161 cccggtcgag cgacgacgac gcggtggggg cccgctcggg cgaggatctt gcacaggctg
     2221 ctcgacacgc cccagccgcg atgcagcacg accaggaacg cggcgttcag accggcgggg
     2281 agcgcgccga gcagcgcgca cagatccacg aggccatcgg cgccggacgc gccgatcgcc
     2341 acgaaccacg gcggatcgag gacccgctgc agctcgaacc ggacctgctt cggcaccagc
     2401 ggcttcttgc acaaggcggc ttggatgaac cgctcgggca cgacgctgtc gtggccggtg
     2461 atgaacagaa acggcgcgcc ccgcgcggtc aacgcatcgg cgacgggaaa caccatctcg
     2521 tcgcgcaggt ggatatcgag gatcgccgca tcgacgaccg cgccgtcctg cagcaggcgc
     2581 agcgcctgat cgatcgacgg agccgggccg acgagcaagt tccctgcgcg ctggatcgtt
     2641 gcaccgagct cggcggcgag cagatagtcg tcctcgacga tcaggatccc gtgtcttcgc
     2701 aagatgggcg tcatggcgtg agcagcgccc cggcgagcgc ggtgggctcg accggcttgt
     2761 cgtagatccg gatgcgccgc ccggcgaggg tcgtcatcct cgaccctcct tgccggcatc
     2821 cttgccgccg attcgatcgt gaaacagcgg tccgaacgcc tcgaccgagt cgcagctcgc
     2881 caggggtctt gctcgcgatc gcgcgcacga cgccgagcag gttgcgggta cgatgctgca
     2941 gctccgcgac caggacgcgc tccggcgtcg cgagatgacc ggtctggatc ccccggtgcg
     3001 cgaccagccg gtagtcatcg gtcaccggat cagcgctccg agcgcgccgc cgccttgcaa
     3061 gaagtccgcc tcgacagcca tcggcatgag ggcaccatga tctggaagcg cactctccac
     3121 aagagaattc gaggacccct gatccgagca agtcgagcaa gctccgacgc ggccgttcgg
     3181 tcgtcgcggt ctcatgtcgg gcgacgaacg gcagccgcgc tcatgcgccg catccccgat
     3241 cggtgtgcgg tggagcgcgg tgacggcgag caccgggcga cggcgacacc gagcacgcgg
     3301 tcacggctcg ccgtcgggat cccagccggt ggcgaggcct tcctcgagct cgtcgagccg
     3361 ggccatcgcg gcggcgtcga gcgcgaagtc gaacaccgcc gcgttctcgg cgatgcgctc
     3421 gcggtggacc gacttgggta gcacgacgaa gccgtgctgg atgccccagc gcaagaggac
     3481 ctgggccggg gtgcggccgg tggcgcgcgc gatcgccgcg accgccgggt ggccgaggcg
     3541 ctggccgcgg gtcagcgggc tgtaggcctc gatcgcgatg ccgtgctcgc ggcacagcgc
     3601 gcaggtgtcg cggcgctgga ggaacggcga cagctcgatc tggttgacgt gggggatgac
     3661 cttggcctcg ccgagcagct cgcgcaggtg cggcaccagg tagttgctga cgccgatcgc
     3721 gcgggccttg ccgtcggaga acgcgcgctc gagcgcccgc cacgagtcgc ggcgcttgcc
     3781 cgggaccggc cagtggatca ggtagaggtc gacgtggtcc atgccgagcc gggccaggct
     3841 cttgtcgagc gcgcgcagcg cgtcgtcgta gccgtggtcg tcgttccaca gcttggtcgt
     3901 gacgaacacc tgatcgcggg gcacgccgcc ggcgcggatc gcggcgccga cgtcggcctc
     3961 gttgccgtag atccgcgcgg tgtcgacgtg gcgatagccg agctcgagcg ccgcggtgac
     4021 ggcgtcgcgg gtggccgcgc cggccggaga caggtagacg ccgaggccga cctgggggag
     4081 ctgtgcgccg gtggcgagcg cgatgcgcgt ggtggtgacc gacatggccg aactataagg
     4141 gtacgccggg aatatgcccc gtacggacgc cggtaagacg gtcatgtcga gctcccctcc
     4201 gcccgcaggg tcccggatcc gccggatcgc gcgcgaggtc gtgatggtcg cggtgttcgc
     4261 gctggtgctg atgtcggcgc gctcgtcgct cgcggaccac tacctcgtgc cgaccggctc
     4321 gatgcagccg accgtcgaga tcgacgatcg cgtgctggtc aacaaggcgg cctacgggct
     4381 gcgcgtgccg ttctcgcaca gctacctggc ccggttcgac gggccggcgg ccggcgacgt
     4441 ggtggtgctc gacgcgccgg acgaggacaa ggtcctgctc aagcgggtgg tcggcgtccc
     4501 gggcacgcgg gtcgaggtcc gcggcggccg gatccggatc gacggcaagc ccgcgccggt
     4561 cgcgcagcgc ggcgacgagc tgttcgagca gctcggccgg gccgatcacg cgctgcggct
     4621 gacccgcggc ggcgggcccg actgggggcc ggtcacgatc cccgagggcc gctacctggt
     4681 gatgggcgac aaccgcggcg acagccgcga tggccggttc ttcgggctgg tgacgcgcga
     4741 ggcgatcctg ggccgggcgg tcggggtgtt ctggcggtcc ggtcccacct gggacggcct
     4801 gtagccgccg atcgacggtc tggacacgcg gcgacgcccc gcggtactca ccggaggtgg
     4861 cgatcttgcg agcctcgcgc gccgtcggcg gcctccgggg cctcgcgctc gcggcgctcg
     4921 cggcgtgcgg cgggccgggc gtgatccatc cccggccgcc ggactgccgg atcgaggtgt
     4981 tcccgagccc gcccggcgac gcctacgtcc aggtcggcga gatggacttc ggcgagtacg
     5041 tcatccacaa gccgcagtac cagtacaaga gcccgtacac gctggccgcc gacatgcggc
     5101 cgcagatctg cgcggtcggc ggcgacacgc tggtggccga gcaagacgcc acgggggtga
     5161 tcgtccgcgc cacggtgtat cgccacgcca acgagtccga catcccggcg ccgtacccca
     5221 agcagccgcc gcccaaggcg gagagctgcg agggtgggtg cggccccggc ttcgcgtgcg
     5281 agcgcgacgt ctgcgtcccg caggccccga cgtgcgatcc ggcgtgcggc gacggcgaga
     5341 cctgcggcgc cgaccggctg tgtcacccgg acagctgagc gtcgcgccac agcacgccgc
     5401 ggtcccggcg atccggtgtg cggccacgca cccgccgcgc gctcgtacat acggaatgcc
     5461 acccaggagg ctgctcgacg atgtcgaggt gacggtgctc gacgcctacc tcgacgatct
     5521 cgacatcgag ctgacccggg acgacgtcac ggcgtgcgcc gtcgtatccg ggacggagct
     5581 ctgcgaata
//
LOCUS       DASVOR010000827         2541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_446251,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000827 DASVOR010000000
VERSION     DASVOR010000827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446251"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="VF516_16285"
     CDS             complement(<1..1260)
                     /locus_tag="VF516_16285"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8109293.1"
                     /translation="MSIARQAARGVAWNMVWGISTRVVTLVGQLVLARFLLPDDYGAA
                     TDAAIVALTANAFTAFSFGQYLIAKRASAEVAFQAWVIHLALGLVALAVIYPLRGPIG
                     RWLETPELGQYLLGYAVSYLVIDRVRYVPERLLMRALRFRALATINGIGELAYITASL
                     ALVRAWGAYAIVAGAVIKSLITSVLFLSAAPRSEWLVRARLRAGDLRDLLTYGVPIMI
                     AIVTDTAAKKWDNAVVSRLFGGGVVGAYNYAYNLADTPISNVAEHIGEVLMPSFSRME
                     PAQRERAAVRAASLMGLVVSPFGVGLGAVAPTVAAAFFDDRWGRLMPAMLAILSVMFV
                     FRPMTWSVIAYVQAVQRTRIVMLSSLVRAILVLTGVAVCGYAGGPYWACAGAGLGFLV
                     TCAATIVTAGRVTALPVGAYLRAVARPL"
     gene            complement(1266..2138)
                     /locus_tag="VF516_16290"
     CDS             complement(1266..2138)
                     /locus_tag="VF516_16290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8109294.1"
                     /translation="MRDEVLTLGGDGRLVGIVSHPSGGQRVARGTTMAPGTLDPGLIL
                     LNAGVLHRVGPHRLHVTLARRIAGAGLTGLRLDLGGIGDSTAASHTTSFRESAVADTR
                     LAMTGMTEMFGIPRFVLFGICAGADNAIATALADDRVAGIILVDPPTHSTRRSRLRYL
                     YSRVAKRGRPQNVVRWGLKAVERGLQGAIARLDGSEEPARPSGKREVPPLPVYGAQLT
                     RLVERGVRILAIFSGIHGAGYNHPDQLFEWFPALRGRIDHAYFSDANHTFTELSVQAE
                     LVDTVTRWMAKNFG"
     gene            2356..>2541
                     /locus_tag="VF516_16295"
     CDS             2356..>2541
                     /locus_tag="VF516_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109295.1"
                     /translation="MFRVLATQLERAGYAALRFDYSGTGDSLGDSRAATVDAWVGDVA
                     IAADRLRSASGAARLAVV"
BASE COUNT          413 a          898 c          892 g          338 t
ORIGIN      
        1 cagcgggcgc gcgaccgcgc gcaggtaggc gccgaccggc agcgcggtga cccggccggc
       61 ggtgacgatc gtggcggcgc aggtcacgag gaagccgagc ccggcgccgg cgcacgccca
      121 gtacgggccg cccgcgtagc cgcacaccgc gaccccggtc aggaccagga tcgcgcggac
      181 caggctcgac agcatgacga tgcgggtgcg ctgcaccgcc tggacgtagg cgatcacgga
      241 ccatgtcatc ggccggaaca cgaacatcac gctgaggatg gcgagcatcg ccggcatcag
      301 ccggccccag cggtcgtcga agaacgccgc cgccaccgtc ggcgccaccg cgccgaggcc
      361 gacgccgaac ggcgacacca ccaggcccat cagcgaggcc gcgcgcaccg ccgcgcgctc
      421 gcgctgcgcg ggctccatcc gcgagaacga cggcatcagc acctcgccga tgtgctcggc
      481 gacgttgctg atcggcgtgt cggcgagatt gtaggcgtag ttgtaggccc cgaccacgcc
      541 gccgccgaac agccgggaca ccacggcgtt gtcccacttc ttggccgcgg tgtcggtgac
      601 gatcgcgatc atgatcggca ccccgtaggt caacagatcg cggagatcgc cggcgcgcag
      661 ccgcgcgcgc accagccact ccgagcgcgg cgccgccgac aggaacagca ccgaggtgat
      721 cagcgacttg atcaccgcgc cggccacgat ggcgtacgct ccccacgccc ggaccagggc
      781 gagcgaggcg gtgatgtacg cgagctcgcc gatgccgttg atcgtggcca gcgcgcgaaa
      841 ccggagcgcg cgcatgagca gccgctcggg cacgtagcgc acgcggtcga tcacgaggta
      901 cgagaccgcg tagccgagca ggtactggcc cagctccgga gtctcgagcc agcgcccgat
      961 cggcccgcgc agcgggtaga tgaccgcgag cgcgacgagg cccagggcga ggtggatcac
     1021 ccaggcctgg aacgcgacct cggccgacgc gcgcttggcg atcaggtact ggccgaacga
     1081 gaacgcggtg aacgcgttgg cggtcagcgc gacgatcgcc gcgtcggtcg ccgcgccata
     1141 gtcgtcgggg agcaggaacc gggcgagcac gagctgaccg accagcgtca ccacgcgggt
     1201 cgagatcccc cacaccatgt tccatgccac gccgcgcgcg gcttgccggg cgatcgacat
     1261 ccagctcagc cgaagttctt cgccatccag cgcgtgacgg tgtcgaccag ctcggcctgt
     1321 acggacagct cggtgaacgt atgattggcg tcggagaagt acgcatgatc gatccggccg
     1381 cgcagcgcgg ggaaccattc gaacagctgg tcggggtggt tgtagcccgc gccgtggatc
     1441 cccgagaaga tcgccaggat acggacgccg cgctcgacga gccgggtcag ctgcgcgccg
     1501 tagaccggca gcggtggcac ctcccgcttg ccgctgggcc gcgcgggctc ttcgctcccg
     1561 tcgagccgcg cgatcgcacc ctgcagcccg cgctccacgg ccttcagccc ccagcgcacg
     1621 acgttctgcg gcctgccgcg ttttgcgaca cggctgtaaa gataccggag ccggctgcgc
     1681 cgcgtagaat gcgtcggggg atcgaccagg atgataccgg ccacccggtc atccgccagc
     1741 gcggtcgcaa ttgcattatc cgcgcccgcg cagatcccga acagcacgaa gcgtgggatc
     1801 ccaaacatct cggtcatgcc ggtcatcgcg agccgggtgt cggcgaccgc gctctcgcgg
     1861 aagctcgtgg tgtgggaggc cgccgtgctg tcgccgatgc cgccgaggtc gagccgcagg
     1921 ccggtgagcc ccgctcccgc gatccggcgc gccagcgtga catgcaaacg gtgcggtccg
     1981 acgcggtgaa gcacccccgc attcaggagg atcaagccgg ggtcaagtgt ccccggcgcc
     2041 attgtagtgc cgcgcgcaac ccgctgacct ccggacggat ggcttacgat cccgacgagc
     2101 cgcccgtcgc cgccaagcgt gagcacctcg tctcgcacac tcgggtcgat catcggaagt
     2161 ccttgccaca cacgggttgc ggtcatggag ggcgcgattg taaactggtt tgcgttgccc
     2221 ggggtgcgaa tagagtactt cggcgcgcag caccagctgc tcggcgcgct ccaccgtccg
     2281 cagcggctgc gaccgcgcag caccgctgtg ttgttgtgta atccattcgg tgaagaggct
     2341 tctcgcgcac accgcatgtt ccgcgtgctg gcgacccagc tcgagcgcgc gggatacgcg
     2401 gcgctgcggt tcgattactc ggggaccggc gactcgctcg gcgacagccg tgcggccacg
     2461 gtcgatgcct gggtcggcga cgtcgcgatc gcggccgacc ggctgaggtc ggcgtcgggc
     2521 gcggcgcggc tcgcggtggt c
//
LOCUS       DASVOR010000828         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_329593,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000828 DASVOR010000000
VERSION     DASVOR010000828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329593"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..349
                     /locus_tag="VF516_16300"
     CDS             <1..349
                     /locus_tag="VF516_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109296.1"
                     /translation="SSHAGFSMRISNALFASFVALCLALPLAACGSSSPADNDPFDTL
                     QACYDDHHSGTDHLPVQQAIATCCLDHPIGGMAAPTCLTTQADCEAHVHTALPSIAAS
                     DITAACMIYISQK"
     gene            346..1146
                     /locus_tag="VF516_16305"
     CDS             346..1146
                     /locus_tag="VF516_16305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109297.1"
                     /translation="MTAGRTIGAGGRIAVALVGLIAPLMPGVARADHDMAAMSANHQD
                     ASELSVGLSVEAAEFDTTYYVGSYQGVAPSLGWMHGRFGASAMIGLYHLTENGLSTYG
                     AGDAMLGGMATVVDGEALHAGVALHVMLPTGSELDNLGMGHMMATPSVWATWRSAPLI
                     VSVSSGYGRALAGLGGHNHGMMPLVDPMNMQELTFSAAADLDLGHGLQLGGKTLGGVP
                     IGTGQTRVIGGGRLAWGTPRVSTGLEVQVGLAGDPFTVRGVVDTALRF"
     gene            1168..1590
                     /locus_tag="VF516_16310"
     CDS             1168..1590
                     /locus_tag="VF516_16310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109298.1"
                     /translation="MRLVLLGLLFAGCTTTAVPTPTRAVCPTPDPMTLTWDSFGQKFM
                     TEFCTDCHSSTLPHSERNGAPLFHDYDMLMTVLEIPDHIDQWAGAGPAATNTIMPPAR
                     CPSTPGGPIDRDCPQPALQQRMDLSAWLACEVNRPHTF"
     gene            complement(1621..2145)
                     /locus_tag="VF516_16315"
     CDS             complement(1621..2145)
                     /locus_tag="VF516_16315"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8109299.1"
                     /translation="MTRSVLAATAIAALAVIVASGGCSGSPGGPTINSKLTEPTTASA
                     SASSSVVSADILAREPVANTATVKHILISWRDLADSFQGHLDSRAEKRSKADAEAEVR
                     SLIKQLQDGADFDALMKAHSEDLGSAMSGRAFTVTPDAQLVIEFRQLSLRLKPGEFGV
                     CQSDFGFHIIKRIT"
     gene            complement(2142..3053)
                     /locus_tag="VF516_16320"
     CDS             complement(2142..3053)
                     /locus_tag="VF516_16320"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX8109300.1"
                     /translation="MPPPLPFAQLPRAARRVTQQGSSFYVIGDERALLRDAYHIFLRL
                     RWSASIGLIAAGFAVTNLVFAALYYVAGGVAGTDGSLFDALSFSIQTLATIGYGAMYP
                     QSRAATTIMAVQAVASVILTALATGLVFSKFARPTTRVGFSRHAVITQHEGQRTLIFR
                     AGNRRSNVIVEATIHVVAVFTTTTAEGKLFYKAVDLPMVRDRQVGMTRGWTVMHVIDE
                     RSPLHEASADKLKQLEMELYIALTGIDDITMQTVHAIHKYTDDQIRVDHHFEDTLIAL
                     DNGEFVVDMTRFDAVVPDAAARDSVRP"
     gene            3140..3472
                     /locus_tag="VF516_16325"
     CDS             3140..3472
                     /locus_tag="VF516_16325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109301.1"
                     /translation="MPQADEVSLERLVELLPPRIWYLTSNGQDMWCRRPYGFLFSTGQ
                     AAEAFARAMGTGEQLFAIGLDAGALISDDMLAGLRNTAVTRLFIDPAIDPDSGDVHGQ
                     ILRLAPLT"
     gene            complement(3581..>3848)
                     /locus_tag="VF516_16330"
     CDS             complement(3581..>3848)
                     /locus_tag="VF516_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HEX8109302.1"
                     /translation="HGGLSRGVRDAMVQRFQESEGASALVLSLKAGGTGLNLTAADHV
                     FLIDPWWNPAVEDQAADRAHRIGQDKPVMVYRMVARDTVEERIL"
BASE COUNT          549 a         1328 c         1369 g          602 t
ORIGIN      
        1 ttcctctcac gcagggtttt caatgcgtat ctccaacgct ctgttcgcct cgttcgtcgc
       61 gctttgcctc gcactccccc tcgccgcttg cggcagcagc agcccggccg ataacgatcc
      121 gttcgacacg ctccaggcct gctacgacga ccaccacagc ggcaccgacc atctgccggt
      181 ccagcaggcg atcgccacgt gctgcctcga ccatccgatc ggcggcatgg ccgcgccgac
      241 gtgcctcacc acccaggcgg actgcgaagc ccacgtgcac acggcgctgc cctcgattgc
      301 cgcctccgac atcaccgccg catgcatgat ctacatcagc cagaagtgac cgccggtcgt
      361 acgatcggtg ccggcggccg gatcgctgtc gcgctggtgg ggttgattgc cccgctgatg
      421 cccggcgtcg cccgcgcgga ccatgacatg gcggcgatgt cagcgaatca ccaggatgcc
      481 tccgagctct cggtcggcct gtcggtcgag gccgccgagt tcgacacgac gtactacgtc
      541 ggcagctacc agggcgttgc gccgtcgctc ggctggatgc acgggcggtt cggcgcgagc
      601 gcgatgatcg gcctgtatca cctcacggag aacgggctca gcacgtacgg cgccggggac
      661 gcgatgctgg gcgggatggc gacggtggtc gacggcgagg cgctgcacgc cggcgtcgcg
      721 ctccacgtca tgttgccgac cggctcggag ctcgacaacc tgggcatggg ccacatgatg
      781 gcgacgccgt cggtgtgggc gacctggcgc tcggcgccgc tgatcgtctc ggtgagcagc
      841 ggctacggcc gggcgctggc cgggctcggc gggcacaacc acgggatgat gccgctggtc
      901 gatccgatga acatgcagga gctgaccttc agcgccgcgg ccgatctcga cctcggtcac
      961 ggcctgcagc tcggcggcaa gacgctcggc ggcgtgccga tcggcaccgg gcagacccgc
     1021 gtgatcggcg gtgggcggct ggcgtggggc accccgcggg tgtcgaccgg gctcgaggtc
     1081 caggtcgggc tcgccggcga tccattcacc gtgcgcggcg tcgtcgacac cgcgctgcgt
     1141 ttctaggagc cttgctcgga ggttgtgatg cgactcgtct tgcttggctt gctgttcgcg
     1201 gggtgtacca ccacggcggt tccgacgccg accagggcgg tgtgcccgac tcccgacccg
     1261 atgacgctga cctgggacag cttcggtcag aagttcatga ccgagttctg caccgactgc
     1321 cactcgagca cgctgccgca ctccgagcgc aacggtgcgc cgctgttcca cgactacgac
     1381 atgctgatga ccgtgctcga gatccccgat cacatcgacc agtgggcggg ggccggcccg
     1441 gccgcgacca acacgatcat gccgccggcg cgctgcccga gcacgccggg cggcccgatc
     1501 gaccgcgact gcccgcagcc ggcgctgcag cagcgcatgg acctgtcggc ctggctggcc
     1561 tgcgaggtca accggccgca cacgttctga ctcgggggtg aagtgcgtga atgcagcgcg
     1621 ttacgtgatg cgcttgatga tgtgaaagcc gaagtcgctc tggcacacac cgaactcgcc
     1681 cggcttgagc cgcagcgaca gctggcggaa ctcgatcacg agctgggcgt cgggcgtgac
     1741 cgtgaacgcg cggccgctca tcgcgctgcc gaggtcctcg gagtgcgcct tcatcagcgc
     1801 gtcgaagtcg gcgccgtcct ggagctgctt gatcagcgag cggacctcgg cctcggcgtc
     1861 ggccttgctg cgcttctcgg cccgcgagtc gaggtggccc tggaacgagt cggccaggtc
     1921 gcgccagctg atcaggatgt gcttgacggt cgcggtgttg gcgaccggct cgcgggcgag
     1981 gatgtcggcc gagaccaccg acgaggaggc ggaggccgac gcggtcgtcg gctcggtgag
     2041 cttgctgttg atcgtcgggc cgcccgggct tccggagcag ccccccgagg caacaatgac
     2101 tgccagggcg gcgatcgcgg tcgcggcgag cacggagcgt gtcatgggcg cacgctatca
     2161 cgggccgccg cgtccggcac gacggcgtcg aaccgggtca tgtcgacgac gaactcgccg
     2221 ttgtcgaggg cgatcagcgt gtcctcgaag tggtgatcga cgcggatctg atcgtcggtg
     2281 tacttgtgga tcgcgtggac ggtctgcatc gtgatgtcgt cgatgccggt cagcgcgatg
     2341 tagagctcca tctcgagctg cttgagcttg tcggcgctgg cctcgtgcag cgggctgcgc
     2401 tcgtcgatca cgtgcatcac ggtccacccc cgggtcatgc cgacctgccg gtcgcgcacc
     2461 atcggcagat cgaccgcctt gtagaacagc ttgccctccg cggtggtggt cgtgaacacg
     2521 gcgacgacat ggatggttgc ttcgacgatg acattgctgc gccggttgcc tgcccggaag
     2581 atcagcgtgc gctggccctc gtgctgggtg atcaccgcgt ggcgcgagaa cccgacccgg
     2641 gtggtcggtc gggcgaactt gctgaacacc aggccggtcg ccagcgcggt gaggatcaca
     2701 ctggcgacgg cctggaccgc catgatcgtg gtcgccgccc ggctctgggg gtacatcgcg
     2761 ccgtagccga tcgtcgccag ggtctggatg ctgaacgaca gcgcgtcgaa caacgagccg
     2821 tcggtcccgg cgaccccgcc ggccacgtag tacagcgcgg cgaacaccag gttggtcacg
     2881 gcgaacccgg ccgcgatcag cccgatcgac gccgaccacc gcagccgcag gaagatgtgg
     2941 tacgcgtcgc gcagcagcgc gcgctcgtcg ccgatcacgt agaagctcga cccctgctgg
     3001 gtcacccggc gggcggcgcg gggcagctgg gcgaacggca gcggcggcgg cacgccggga
     3061 tcctcggccc ggccgggtgc cgggaccagt tttcggcggc cctcgcggca cccggcggcc
     3121 ggaccgggta caacgggcca tgccccaagc ggacgaggtc tcgctcgagc ggctggtcga
     3181 gctcctgccg ccgcggatct ggtacctgac gtcgaacggc caggacatgt ggtgccgccg
     3241 gccgtacggc ttcctgttct cgaccggcca ggcggccgag gcgttcgccc gggcgatggg
     3301 caccggcgag cagctgttcg cgatcgggct cgacgccggc gcgctgatct ccgacgacat
     3361 gctcgccggc ctgcgcaaca ccgcggtcac ccggctgttc atcgacccgg cgatcgaccc
     3421 cgactcgggc gacgtccacg gccagatcct gcggctcgcc ccgctgacct gaccggctgc
     3481 gctacgcgag cagcgcgagc aggtcgtcgc gggtgatcgc ggcggcggag ccggcctcgg
     3541 cgagcgcggc gtcggccagc gcgcgcttgc ggtcctgcag ctagaggatg cgctcctcga
     3601 cggtgtcgcg cgcgaccatg cggtagacca tcaccggctt gtcctggccg atccggtggg
     3661 cgcgatcggc ggcctggtcc tcgaccgcgg ggttccacca cgggtcgatc aggaacacgt
     3721 ggtcggcggc ggtgaggttg aggccggtgc cgccggcctt gagcgagagc accagcgccg
     3781 acgcgccttc cgactcctgg aagcgctgca ccatcgcatc gcgcacgccg cgggacaggc
     3841 cgccgtgg
//
LOCUS       DASVOR010000829         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_563839,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000829 DASVOR010000000
VERSION     DASVOR010000829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563839"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1063)
                     /locus_tag="VF516_16335"
     CDS             complement(<1..1063)
                     /locus_tag="VF516_16335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109303.1"
                     /translation="MVSSRTCQAAMVVALAAGCSDQAARVQLVPVGGDCGRPMGANQV
                     KVTAYGPGGSRAASVGFDESVAFASFPADTEQFAVEVFIGGGVLAAEGKSAPLAFDAL
                     PDGGKIYVRMAPPDGFCELPPMIEPRSQPLVAPAGNGALVVGGIGPNGPLSTAEYYDP
                     TAAAFVPVEVPSALVDPQGFAGAALATLPDGRVALTGGPGSVFIVFDPTRRAFVTDPT
                     KINGRAFHAAIATGDQEVLLAGGCTDVTMQRCDGVAKMQTERYRLGMLSEPVFSPVLA
                     SGFRTGAQLFDLGVQLDGARRYLLAGGITAASLASVGTPGRADRFALDDQDPAEVVMG
                     GHAQPAALDGSAVLTAFAED"
     gene            1088..>2120
                     /locus_tag="VF516_16340"
     CDS             1088..>2120
                     /locus_tag="VF516_16340"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109304.1"
                     /translation="MPESQRDDPPPAIDLPATFGKYFLTEKLATGGMAEIYLAKIVGP
                     GGFEKQLVIKQIHPRLSGQRHFVDLFVAEAKILVTLAHGNIVPVYELGVIDDTYFIAM
                     EYIDGPTLYRLTETLRRRGVRMAPAVAAWITARILEGLDYAHRKGDGVIHRDLSPRNV
                     MLSRDGEVKLVDFGIAVTLGDAGEDAAGESAPTGSFPYMSPEQVRKQPLTGQTDLFSV
                     GVLLWEMLVGRRLFARSDPDATLRAVTDGDIARPSAGSPEVPARLDDVVMRALERDPA
                     ARWASAGDMLAALQRYLYSLDETPGPRDVAALVARYGPPETRRLPTHHDELDADAAPD
                     APRPRPWTAS"
BASE COUNT          298 a          834 c          730 g          258 t
ORIGIN      
        1 cgtcctcggc gaacgcggtc agcaccgcgc tgccgtcgag cgcggcgggc tgggcatggc
       61 cgcccatcac gacctcggcg gggtcctggt cgtcgagcgc gaaccgatcg gcgcggccgg
      121 gcgtgcccac actggcgaga ctggccgccg tgatcccgcc ggcgagcagg tagcgccgcg
      181 cgccgtcgag ctgcacgccg aggtcgaaca gctgcgcgcc ggtccggaac ccggacgcca
      241 ggaccggcga gaacaccggc tcgctcagca tgccgagccg gtagcgctcg gtctgcatct
      301 tggccacgcc gtcgcaccgc tgcatcgtca catcggtgca gccgccggcg agcagcacct
      361 cctgatcgcc ggtcgcgatc gcggcgtgaa acgcgcgccc gttgatcttc gtcggatcgg
      421 tgacgaacgc gcgccgggtg ggatcgaaca cgatgaacac gctccccggg ccgccggtca
      481 gggcgacccg gccgtccggc agcgtcgcca gcgccgcccc cgcgaacccc tgcgggtcga
      541 ccagcgcgga cggcacctcg accggcacga acgccgccgc ggtgggatcg tagtactcgg
      601 cggtcgacag cggcccgttc gggccgatcc cgccgaccac cagcgcgccg ttgcccgccg
      661 gggcgaccag cggctgcgac cgcggctcga tcatcggcgg cagctcgcag aacccgtcgg
      721 gcggcgccat ccgcacgtag atcttgccgc cgtcgggcag cgcgtcgaac gccagcggcg
      781 ccgacttgcc ctccgcggcg agcacgccgc cgccgatgaa cacctcgacc gcgaactgct
      841 cggtgtccgc cgggaagctg gcgaacgcca ccgactcgtc gaacccgacc gacgcggcgc
      901 ggctgccccc gggcccgtac gcggtcacct tcacctggtt cgcgcccatc ggccgcccgc
      961 agtcgccgcc gaccggcacc agctggacgc gcgccgcctg gtcgctgcag cccgccgcga
     1021 gcgcgaccac catcgccgcc tggcacgtgc gagaggacac caccgaccgc atgatactct
     1081 tgtcgcgatg ccggagagtc agcgggacga tccgcccccc gcgatcgacc tgccggcgac
     1141 gttcggcaag tacttcctga ccgagaagct cgcgaccggc ggcatggccg agatctacct
     1201 ggccaagatc gtcgggccgg gcgggttcga gaagcagctc gtcatcaagc agatccaccc
     1261 ccggctgtcc ggccagcgcc acttcgtcga cctgttcgtc gccgaggcca agatcctggt
     1321 caccctcgcc cacggcaaca tcgtcccggt ctacgagctc ggcgtgatcg acgacacgta
     1381 cttcatcgcc atggagtaca tcgacggccc gacgctgtac cggctgaccg agacgctgcg
     1441 ccggcgcggc gtgcggatgg cgccggcggt cgcggcgtgg atcaccgcgc gcatcctcga
     1501 gggcctcgac tacgcgcacc gcaagggcga cggcgtgatc caccgcgacc tgtcgccgcg
     1561 caacgtgatg ctgtcgcgcg acggcgaggt caagctggtc gacttcggga tcgcggtcac
     1621 gctgggcgac gccggcgagg acgccgccgg cgaatcggcg ccgaccggct cgttcccgta
     1681 catgtcgccc gagcaggtcc gcaagcagcc gctgaccggg cagaccgacc tgttctcggt
     1741 cggtgtcctc ctgtgggaga tgctggtcgg ccgacgcctg ttcgcgcgca gcgatcccga
     1801 cgccacgctg cgcgcggtga ccgacggcga catcgcgcgc ccgtcggccg gcagccccga
     1861 ggtcccggcc cggctcgacg acgtcgtgat gcgcgcgctc gagcgcgatc cggcggcgcg
     1921 ctgggcctcc gccggcgaca tgctcgccgc gctgcagcgc tacctgtaca gcctcgacga
     1981 gacccccggg ccgcgcgacg tcgcggcgct ggtcgcgcgc tacggcccgc ccgagacccg
     2041 ccggctgccg acccatcacg acgagctcga cgccgacgcc gcccccgacg ccccgcggcc
     2101 ccggccgtgg accgcgagcc
//
LOCUS       DASVOR010000830         2932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_475923,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000830 DASVOR010000000
VERSION     DASVOR010000830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2932
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_475923"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..1809
                     /locus_tag="VF516_16345"
     CDS             187..1809
                     /locus_tag="VF516_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEX8109305.1"
                     /translation="MIAFSSVSKQYGGQILFVDASFQINPGEKVGLVGPNGAGKTTVF
                     RLITGEDQPDDGAIERPKKLTLGYFRQDVGELRGRSILAETCAGAGEVARLGEELAAL
                     TARLEAAGDDLDDVVARFGEVQARYQDLGGYELEARAQTILHGLGFADDQMQNDVGTL
                     SGGWKMRVALAQILLARPELLLLDEPTNYLDLESILWLEGFLRDYTGTVVMTCHDREI
                     MNRVVGKIVEIDGGALRSYSGNYEFYERMRDLEAARREAEYARQQARLAKESRFIERF
                     KTHVAKAAQVQSRLKKLDKVEKIAEPRRIVEKRFEFRTPARSGDDVVKLSGLRKAYGA
                     RVVHAGLTLTMRRRERWAVMGENGAGKSTLLKMIAGALPPDAGEAAIGASVALGYFAQ
                     HVMDQLSGDRTVLEELTEHAPAANQGTLRNLAGAFGFHDDDVFKPVRVLSGGEKARLA
                     LAKLLYDAPNLLVLDEPTNHLDIATKRALVAALADYEGTLVFVSHDRQFLRALANRVL
                     ELSAAGPQIYGGSYDEYVSSTGREAPGMRAPS"
     gene            complement(1829..>2932)
                     /locus_tag="VF516_16350"
     CDS             complement(1829..>2932)
                     /locus_tag="VF516_16350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109306.1"
                     /translation="HQAPHGALGARVSALWLLAAACGGAGSPPAAPAQPTVDARTAEK
                     DARGLVVEIFQTIDRGNTDSMFSLLADPLTVLGPRRADAMATRSDALVALGKVVDRRA
                     PKTKSHPQLRSTALTVAVSPGGRSAWAFDVVRFEGQSIAVTAILSNTSDLWSVTTAAL
                     ALVPSAARARTESARDAIVPPGVPPGGAAAGKTDPAAARVVEDWKKGLLDPDRWGTEL
                     MSQSDAIYAGPTAGQVAHGKQAIKQLWKARTKANVREAVSGEITARVTPDGQLVWLSA
                     PVTRVADGEDPLPLRSFAVYEKDGAAWQLIVLHEGLAIDEPGSGTAYKKIVPAPPRAE
                     PAKPDDKKPGDTATAKAKPARKKKKPAPKPDDP"
BASE COUNT          404 a         1047 c         1070 g          411 t
ORIGIN      
        1 cccacggcgt cgcctcggcg aggatccggt ggatcgcgcg cgcgctggcc tcggtcagcg
       61 cgccgcggac ctggacccgg cggtcgcgcg cgtacgcggc ggtgagcgcg ggcacatcga
      121 ggtccgggtt gatatcgagc aggtgcggtc cgtccatggc cctagcatgc tatagaccgc
      181 gccgacgtga ttgcgttcag ctcggtcagc aagcagtacg gcggccagat cctgttcgtc
      241 gacgcctcgt tccagatcaa cccgggcgag aaggtcgggc tggtcggccc caacggcgcc
      301 ggcaagacca ccgtgttccg gctgatcacc ggcgaggacc agcccgacga cggcgcgatc
      361 gagcggccga agaagctgac cctggggtac ttccggcagg acgtcggcga gctgcgcggg
      421 cggtcgatcc tggccgagac ctgcgccggc gccggcgagg tcgcccggct cggcgaggag
      481 ctcgcggcgc tgaccgcgcg gctcgaggcc gccggggacg atctcgacga cgtggtcgct
      541 cggttcggcg aggtccaggc ccgctaccag gacctcgggg gctacgagct cgaggcccgc
      601 gcccagacga tcctgcacgg cctggggttc gccgacgacc agatgcagaa cgacgtcggc
      661 acgctgtccg gcggctggaa gatgcgggtc gcgctggccc agatcctgct cgcccgcccc
      721 gagctgctgc tgctcgacga gccgaccaac tacctcgacc tcgagtcgat cctgtggctc
      781 gagggcttcc tgcgcgacta caccggcacg gtcgtgatga cctgtcacga tcgcgagatc
      841 atgaaccgcg tggttggcaa gatcgtcgag atcgacggcg gcgcgctgcg ctcgtacagc
      901 ggcaactacg agttctacga gcggatgcgc gacctcgagg cggcgcgccg cgaggccgag
      961 tacgcccgcc agcaggcccg gctggccaag gagtcccggt tcatcgagcg gttcaagacc
     1021 cacgtcgcca aggcggccca ggtccagagc cggctcaaga agctcgacaa ggtcgagaag
     1081 atcgccgagc cgcgccggat cgtcgagaag cggttcgagt tccgcacccc ggcgcggtcg
     1141 ggcgacgatg tcgtcaagct gagcgggctg cgcaaggcgt acggggcgcg cgtcgtccac
     1201 gccgggctga ccctcaccat gcggcgcagg gagcgctggg cggtgatggg cgagaacggg
     1261 gccggcaagt cgaccctgct caagatgatc gccggggcgc ttccgcccga cgccggcgag
     1321 gcggcgatcg gcgcgtcggt cgcgctcggc tacttcgcgc agcacgtcat ggaccagctg
     1381 tccggagacc ggacggtgct ggaggagctg accgagcacg cgcccgccgc caaccagggc
     1441 acgctgcgca acctcgccgg cgcgttcggg ttccacgacg acgacgtgtt caagccggtc
     1501 cgcgtgctgt cgggcggcga gaaggcgcgg ctcgcgctgg ccaagctgct ctacgacgcg
     1561 ccgaacctgc tggtgctcga cgagccgacc aaccacctcg acatcgccac caagcgcgcg
     1621 ctggtcgccg cgctggccga ctacgagggc acgctggtgt tcgtgtcgca cgatcgccag
     1681 ttcctgcgcg cgctcgccaa ccgcgtgctg gagctgtcgg ccgccgggcc gcagatctac
     1741 ggcggcagct acgacgagta cgtcagctcg accggccgcg aggcgcccgg catgcgcgcg
     1801 ccgtcgtgac ccggccgcac ccctggcatc agggatcgtc gggcttgggc gccggcttct
     1861 tcttcttcct ggccggcttg gccttggccg tcgcggtgtc gcccggcttc ttgtcgtcgg
     1921 gcttggccgg ctcggcccgg ggcggcgccg ggacgatctt cttgtaggcg gtgccgctgc
     1981 ccggctcgtc gatcgcgagc ccctcgtgca ggacgatcag ctgccacgcc gcgccgtcct
     2041 tctcgtacac cgcgaagctg cgcagcggca gcggatcctc gccgtcggcg acccgggtga
     2101 ccggcgcgct cagccacacc agctggccgt cgggcgtgac ccgtgcggtg atctcgccgc
     2161 tgaccgcctc gcggacgttg gccttggtcc gcgccttcca cagctgcttg atcgcctgct
     2221 tgccgtgcgc gacctgccct gccgtcgggc cggcgtagat cgcatcgctc tggctcatca
     2281 gctcggtgcc ccagcgatcc ggatcgagca ggcccttctt ccagtcctcg accacgcgcg
     2341 cggcggccgg atcggtcttg cccgcggcgg cgccgccggg cgggacgccg gggggaacga
     2401 tcgcgtcgcg cgccgactcg gtcctggcgc gcgccgcgct cggcaccagc gcgagcgccg
     2461 cggtggtcac cgaccacagg tccgaggtgt tcgacaggat cgcggtcacg gcgatggact
     2521 ggccctcgaa gcggacgacg tcgaacgccc acgccgagcg gccacccggc gacaccgcga
     2581 cggtgagcgc ggtcgaccgc agctgcgggt ggctcttggt cttcggggcc cggcgatcga
     2641 cgaccttgcc gagcgcgacc agcgcatcgg agcgggtggc catcgcgtcg gcgcggcgcg
     2701 ggccgagcac ggtcagcgga tcggcgagca gcgagaacat cgagtcggtg ttgccgcggt
     2761 cgatggtctg gaagatctcg acgaccaggc ccctggcgtc cttctcggcg gtgcgcgcat
     2821 cgaccgtcgg ctgcgccggc gcggcgggcg gcgaaccggc gccaccgcac gcagcggcga
     2881 gcagccacag cgcggaaacc cgtgccccta gcgcgccatg cggcgcttga tg
//
LOCUS       DASVOR010000831         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387832,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000831 DASVOR010000000
VERSION     DASVOR010000831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387832"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..631)
                     /locus_tag="VF516_16355"
     CDS             complement(158..631)
                     /locus_tag="VF516_16355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109307.1"
                     /translation="MTAPATSGATVSASEEAELERESYGWQIAIADVAGTLLLFRNDH
                     SSASVGLMVYALGGPIIHGAHEQGGRAVASLTLRLGLPLASAWVWGRCPSSADECEND
                     GAVAVGLVLGFVTAMVIDATALSHPVKVKKPARMTWAPHVAATRQQVGLGVLGQF"
     gene            1106..1372
                     /locus_tag="VF516_16360"
     CDS             1106..1372
                     /locus_tag="VF516_16360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109308.1"
                     /translation="MPLTIIDHAASTGVLRDTYDRMLERPLPPVYRPRHGGAPGIIQA
                     HSLDPLLIPKVFGCSSTLNGAGPLSWPERELVNAVTSRLNQCLY"
BASE COUNT          218 a          566 c          567 g          219 t
ORIGIN      
        1 gtacgagccg ttcatcaccg ggtggctcga cgagcagacc gaccaggcgt ggggccggcc
       61 ggccgacctc gagatcctcg acgacggctc gctgctgatc agcgacgact acaagggcgt
      121 gctgtaccgc gtgacctacc ggaagtagcc gccgcgctca gaactgcccg agcacgccga
      181 gcccgacctg ctggcgcgtc gccgcgacgt gcggggccca ggtcatgcgg gcgggcttct
      241 tgaccttcac cgggtgcgac agcgcggtcg cgtcgatcac catcgcggtg acgaagccca
      301 ggacgaggcc gacggccacg gcgccgtcgt tctcgcattc atcggcgctc gaggggcagc
      361 gcccccacac ccacgcgctg gccagcggca acccgaggcg cagcgtcagg ctggccacgg
      421 cgcggccccc ctgttcgtgc gcgccgtgga tgatcgggcc gcccagcgcg tagaccatca
      481 ggccgacgga cgcgctgctg tgatcgttcc tgaacagcag cagcgtgccg gccacgtcgg
      541 cgatcgcgat ctgccagcca tagctctcgc gctcgagctc ggcttcttcg ctcgcggaca
      601 ccgtcgcgcc ggaagtcgcc ggcgccgtca tcgacggcgg ggtgagcgtg ggcggggtcg
      661 gagccgcagc gcggaccggc gtcgcgggcg aggtggtcga cggcggcgct ggcacagcgg
      721 agggcggcgg tgacggtgcg ggtgccggcg ctgacgctgg tgcgctcgat tctgcaggcg
      781 aggaatccgc atccggcggc ggtgcagctt ctggcggcgc cggcgcgggc actgcttctc
      841 ccggcggcgc gctgtccggc ggtgcatccg gcggagccgg ctgtgcgctc gccgtcgaga
      901 tcatggccat ccaacccagg aaccagacaa tccgcatggc gaccggcact acaaggagca
      961 tgccacgcgg aagtgcgtga cgatcctcgg ccgctgcggg agcggcgcga catggccggc
     1021 agcgccccgg ccggcaccgc gacacgcgcg tcgcgatcgg cattgttgcc gtcgaccacg
     1081 tgacgtaccc tggcctcccc ctgcgatgcc actgaccatc atcgaccacg cagcctcgac
     1141 cggcgtgctg cgcgacacct atgaccggat gctggagcgt ccattgccac cggtgtaccg
     1201 gccgcggcac ggcggggcgc ccggcatcat ccaggcccac agcctcgatc cgctgctgat
     1261 ccccaaggtg ttcgggtgct cgtcgaccct caacggcgcg gggccgctga gctggcccga
     1321 gcgcgagctg gtcaatgcgg tgacctcccg gctcaatcaa tgcctgtatt gaacggcgtg
     1381 tcacgcagag tttctgcgtg tcgcgctgga cggtcgtgac gagctgtcgc gggcggtgat
     1441 cgccgatccg cacgccgtcg cagcgcgctg gccggacgac cgccggcgca tgctcgccga
     1501 gatcgcgagc gtggtcaccc tggcgccgtg ggcgctgtcg cccggccatc gcgcccgagc
     1561 gcgcggcgcc
//
LOCUS       DASVOR010000832         2249 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_416730,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000832 DASVOR010000000
VERSION     DASVOR010000832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416730"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VF516_16365"
     CDS             <1..475
                     /locus_tag="VF516_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109309.1"
                     /translation="LGRAVATLAEGVATLAALREGELGWTIAAALRAIDLPIPSGLNS
                     EIAGEDASIAERARILKKEMSRKAKATVQHLAQTRPGELAQVEAFRLGALAIADRAGL
                     LWAGDLAVALAQLDVGRGGKALIDSRGALELTAWSVSEDHLQLRERLAIGLKGVR"
     gene            472..>2249
                     /locus_tag="VF516_16370"
     CDS             472..>2249
                     /locus_tag="VF516_16370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109310.1"
                     /translation="MTTPKGPSKPGPKPFLGDDDLSELDAWVETFDALHGGPELSAEP
                     PGELDATQLVSSLAQTGVTQPPDGGLDAPPDLLDLPEPSARPTPPVAGSFDLSRAPSV
                     TAIDSDPDEGEAFGGDDDDDDEVFTSASRPGAPAPEDALFDDDIAPPPAPEPLRRGPA
                     IVRRNSAQTPTPPTDHLAISEAPHSRRDSDAMFSEQTRVADFGELEQRLHSSREMPAA
                     APDDDDYLDIEVSDEKAAAPEVPVFEPFPTGRRTAHVVRRTEPGRGPSGQRPVVELDL
                     EDDLSPARPLSPPASEDDFSDVAAAVGASDDLAVPDIPQRRPVPRGGSIPEIAVDVRP
                     RANLDDALAGLRSEESGEFGGRHPFDEPQQFGDEFGDPDQFDGPDQFANPQQFSDEFG
                     DPDQFDEPQQFAPSPFGAPNQFGAPNQFDAPNQFDEPQHFDEPNRFAASGFDMPDPFA
                     AGDEFGDPDQFDDPHAFDEPQPGPALPPIAPLTPSGAVRRPVPSSETVRGMSISQIAE
                     AGMLRPPAITDLYPRIKTPTSVPPIGGPSSPPPGVAPIPAGQVPSHTILEFGLPSRPA
                     RPTRPPPQPPPLQPVSQPGIPLPPPAA"
BASE COUNT          310 a          909 c          773 g          257 t
ORIGIN      
        1 gctcggccgc gcggtcgcca cgctcgccga gggcgtcgcc acgctcgccg cgctgcgcga
       61 gggcgagctc ggctggacga tcgccgccgc gctgcgcgcg atcgacctgc cgatcccgtc
      121 cgggctgaac agcgagatcg ccggcgagga cgccagcatc gccgagcgcg cccggatcct
      181 gaagaaggag atgagccgca aggccaaggc gaccgtgcag cacctggccc agacccgccc
      241 cggcgagctc gcgcaggtcg aggcgttccg gctcggtgcg ctcgcgatcg ccgaccgcgc
      301 gggccttctg tgggcgggcg acctcgcggt ggcgctcgcc cagctcgacg tcggccgcgg
      361 cggcaaggcg ctgatcgaca gccgcggcgc gctggagctg accgcatggt cggtgtccga
      421 ggaccacctc cagctgcgcg agcggctcgc catcgggctg aaaggcgtac gatgacgacc
      481 cccaagggac ccagcaagcc cggccccaag ccgttcctcg gcgacgacga cctgtccgag
      541 ctcgacgcct gggtcgagac cttcgacgcg ctgcacggcg gccccgaact ctccgccgag
      601 ccgccgggcg agctcgacgc gacccagctg gtgtcctcgc tggcccagac cggcgtcacc
      661 cagccgccgg acggcggtct cgacgcgcca cccgatctgc tcgacctgcc cgagccgagc
      721 gcacggccga ctccgccggt ggccggcagc ttcgatctct ctcgcgcgcc gtcggtgacc
      781 gcgatcgaca gcgatccgga cgagggcgag gcgttcggcg gcgacgacga cgatgatgac
      841 gaggtgttca cctcggcgtc gcggcccggt gccccggccc ccgaggacgc gctgttcgac
      901 gacgacatcg cgccgccacc ggcgcccgag ccgctgcgcc gcggcccggc gatcgtgcgc
      961 cgcaactcgg cccagacccc gacgccgccg accgatcacc tcgcgatcag cgaggccccg
     1021 cactccaggc gcgactccga cgcgatgttc tccgagcaga cccgggtcgc cgacttcggc
     1081 gagctcgagc agcggctgca ctcgtcgcgc gagatgccgg cggcggcgcc ggacgacgac
     1141 gactacctcg acatcgaggt cagcgacgag aaggccgccg cgcccgaggt cccggtgttc
     1201 gagccgttcc cgaccggtcg gcgcaccgcc cacgtcgtgc gccgcaccga gcccggccgc
     1261 ggaccatccg gccagcgccc ggtggtcgag ctcgacctcg aggacgatct gtcgccggcc
     1321 cggccgctgt cgccaccggc ctcggaggac gacttctccg acgtcgccgc cgcggtcggc
     1381 gccagcgacg acctcgcggt gcccgacatc ccgcagcgcc ggccggtccc ccgcggtggc
     1441 tcgatcccgg agatcgcggt cgacgtgcgg ccgcgggcca acctggatga cgcgctcgcc
     1501 gggctgcgca gcgaggagtc gggcgagttc ggcggccgtc acccgttcga cgagccgcag
     1561 cagttcggcg acgagttcgg cgatcccgac cagttcgacg gcccggacca gttcgccaac
     1621 ccgcagcagt tcagcgacga gttcggcgat cccgaccagt tcgacgaacc ccagcagttc
     1681 gcgcccagcc cgttcggcgc gcccaaccag ttcggcgcgc ccaaccagtt cgacgcgccc
     1741 aaccagttcg acgagccgca gcacttcgac gagcccaacc ggttcgcggc cagcgggttc
     1801 gacatgcccg acccgttcgc cgcgggcgac gagttcggcg atcccgacca gttcgacgat
     1861 ccgcacgcgt tcgacgagcc gcagccgggc ccggcgctgc cgccgatcgc cccgctgacc
     1921 ccgtccggcg cggtgcgccg gcccgtgccg agcagcgaga ccgtgcgcgg gatgtcgatc
     1981 agccagatcg ccgaggccgg catgctgcgg ccgcccgcga tcaccgacct gtacccgcgg
     2041 atcaagaccc cgacgtcggt gccgccgatc ggcggcccat cctcgccacc gccgggggtt
     2101 gcgccgatcc cggccgggca ggtcccgagc cacacgatcc tcgagttcgg actgccgtcc
     2161 cgtcccgcgc ggccgacccg gccgccgccc cagccgccgc cgctccagcc ggtgagccag
     2221 cccggaatcc cgcttccgcc cccggcagc
//
LOCUS       DASVOR010000833         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_8614,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000833 DASVOR010000000
VERSION     DASVOR010000833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8614"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..>1525)
                     /locus_tag="VF516_16375"
     CDS             complement(365..>1525)
                     /locus_tag="VF516_16375"
                     /inference="COORDINATES: protein motif:HMM:NF005557.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxyadenosine deaminase"
                     /protein_id="HEX8109311.1"
                     /translation="CVVMPGLVQAHVHTCQTLARGRADDLELLDWLRTVIWPYEAALD
                     EAALTASAELACAELLLGGTTAILDMATVHHPDAVFAAAERAGIRATIGKAMMDAPDP
                     QIPPGLRESTRASLDESAALIARWHGAAGGRLRYAYAPRFVLSCTDDLLREVGAEARA
                     RGVRIHTHASESTGEVALVRQRFGKDNIAVLDQLGLLGDHCCIAHCIHLSADERRLLA
                     ARGAHVCHCPSSNLKLASGICDVPALIAAGVSVAIGADGAPCNNNLDGLLELRLTALL
                     HKHRTGPRALPAPEVVRMATLGGAAALGLADQIGSLEPGKRGDVIAVDVTAIHTVPTA
                     SPWSAVAYAAHAGDVRHVAVDGALVVRDGELRTLERFRVRERAQAAAARLFR"
BASE COUNT          209 a          546 c          584 g          186 t
ORIGIN      
        1 ctcgcccagc gcggtcgaca ccgacggcac gagcccggcg agcgcgggat tcatcatcgc
       61 cggcctccgc catcgcggag gtcgaccgcc ggaacgtgac ggacgtgacg gagcgggctt
      121 ccagcgtaca gacgactcga catgggttct cgacttgcgc gtggctccga tccagcacgg
      181 cagacccccg gagccacggg ggcatgtcct tcggaccgca ccgcggcccg gcgccctgac
      241 tgaacggctc accgccgacg atcaataaaa cgcaacagcg gtctagcacg tacatctcgg
      301 agcgtctagc agaacgcggt cagagcttcc cgcacgcgcg gcgcgcgcga ccggcgcacg
      361 ctcgtcagcg aaatagccgc gccgccgcgg cttgtgcgcg ctcgcggacc cggaaccgct
      421 cgagcgtgcg gagctcgccg tcgcgcacga ccagcgcgcc gtcgaccgcg acgtggcgga
      481 cgtcaccggc gtgcgcggcg taggcgaccg ccgaccacgg gctcgcggtc ggcacggtat
      541 ggatcgcggt gacgtcgacc gcgatcacgt cgccgcgctt gccgggctcg agcgagccga
      601 tctggtcggc gaggccgagc gccgccgcgc cgcccagcgt ggccatccgg accacctcgg
      661 gcgcgggcag cgcgcgcggc ccggtgcggt gcttgtggag cagcgcggtg agccggagct
      721 cgagcaggcc gtcgaggttg ttgttgcacg gcgcgccgtc ggcgccgatc gcgaccgaca
      781 cgccggcggc gatcagcgcg gggacgtcgc agatccccga cgcgagcttg aggttcgacg
      841 atgggcagtg gcagacgtgg gcgccgcgcg cggcgagcag ccggcgctcg tcggccgaca
      901 ggtggatgca gtgggcgatg cagcagtggt cgccgagcag gccgagctgg tcgagcacgg
      961 cgatgttgtc cttgccgaac cgctggcgga ccagcgcgac ctcgccggtg ctctcgctgg
     1021 cgtgggtgtg gatccgcacg ccgcgcgcgc gggcctcggc gccgacctcg cggagcaggt
     1081 catcggtgca cgacagcacg aaccgcggcg cgtacgcgta gcgcagccgg ccgccggcgg
     1141 cgccgtgcca gcgcgcgatc agcgcggccg actcgtcgag cgacgcccgg gtcgattcgc
     1201 gcagtccggg cgggatctgc gggtcgggcg cgtccatcat cgccttgccg atcgtcgccc
     1261 ggatcccggc gcgctcggcg gcggcgaaca ccgcgtcggg gtggtggacg gtcgccatgt
     1321 cgaggatcgc ggtggtcccg ccgagcagga gctcggcgca cgccagctcg gcgctcgcgg
     1381 tcagcgcggc ctcgtcgagc gcggcctcgt acggccagat cacggtgcgc agccagtcga
     1441 gcagctcgag gtcgtcggcc cggccgcgcg ccagggtctg gcaggtgtgg acgtgggcct
     1501 ggaccaggcc gggcatcacc acgca
//
LOCUS       DASVOR010000834         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_66542,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000834 DASVOR010000000
VERSION     DASVOR010000834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66542"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VF516_16380"
     CDS             <1..257
                     /locus_tag="VF516_16380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109312.1"
                     /translation="PCEWFDGQGREVARGTFAHGAPRDGTFLDWSRYQPDLKDPWTVA
                     QHGKDWITLFEASYLSQSPDYQQVRVAYQAGVRVEPRTSP"
     gene            complement(261..1604)
                     /locus_tag="VF516_16385"
     CDS             complement(261..1604)
                     /locus_tag="VF516_16385"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8109313.1"
                     /translation="MSAILVVDDERGIRALCSDVLGRAGYEVESVDSAAGALAAVTRR
                     RFELILCDINLPDQDGVSLLPRLLVGDPPPAVLLITAYPSIDTAVRGMKLGARDYIGK
                     PFSPDELRLVIRRALDEDELRRAHGELTKRLAYGSMIGDSPAMQQLRETIDKVAKADA
                     TVLITGESGTGKELVARALHFAGRRASRSFVPVNCGALVGTLLDSELFGHLRGAFTGA
                     DTAKRGLFLAADGGTLFLDEIGELPLELQPKLLRALQDGEIKPVGGTASVRVDVRVIA
                     ATNRALDEAVKAGTFREDLFYRLAVITLEVPPLRNRPGDIAQLARHFADDAALRAERG
                     RLELTDAAIAYLAAQPWPGNVRELENTIERAVILATGNVLDIADVAAKRPATPAATLQ
                     TFSGDHVPSLDELERAHILRVLELCEGQKTKAAALLRINRTTLWKKLRQYGIEGE"
     gene            1906..2148
                     /locus_tag="VF516_16390"
     CDS             1906..2148
                     /locus_tag="VF516_16390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109314.1"
                     /translation="MPVGTPDDMAPEVWLGEPPGRRTDVDSLGALRFELLAGAPPFAD
                     VEPAAAALGGSISCPVERREITGPTPARRPRNLYNT"
BASE COUNT          400 a          885 c          936 g          407 t
ORIGIN      
        1 gcccctgcga atggttcgac ggacagggcc gcgaggtcgc gcgggggacg ttcgcgcacg
       61 gggcccctcg ggacggtacc ttcctcgact ggtcgaggta ccagcccgac ctcaaagatc
      121 cctggacggt cgcgcagcac ggcaaggact ggatcacgct gttcgaggca agctatctct
      181 cgcagtcgcc cgattatcag caggtccggg ttgcttacca ggccggtgtg cgggtggagc
      241 ctcgtacgtc accctgaaca tcactcgccc tcgatgccgt actggcgcag cttcttccac
      301 agcgtggtgc ggttgatccg gagcagggcg gcggccttgg tcttctggcc ctcgcacagc
      361 tcgagcacgc gcaggatgtg ggcgcgctcc agctcgtcga gcgacggcac gtggtcgccg
      421 ctgaacgtct gcagcgtggc cgccggggtc gccggccgct tggcggcgac gtcggcaatg
      481 tcgagcacgt tgccggtcgc caggatcacg gcgcgctcga tcgtgttctc gagctcgcgc
      541 acgttgcccg gccacggctg ggcggcgagg taggcgatcg cggcgtcggt cagctcgagc
      601 cggccgcgct cggcgcgcag cgcggcgtcg tcggcgaagt gccgcgcgag ctgcgcgatg
      661 tcgcccggcc ggttgcgcag cggcgggacc tcgagcgtga tcaccgcgag ccggtagaac
      721 aggtcctcgc ggaacgtgcc ggccttgacc gcctcgtcga gcgcccggtt ggtcgcggcg
      781 atcacccgga cgtcgacccg gaccgaggcc gtgccgccga ccggcttgat ctcgccgtcc
      841 tgcagcgcgc gcagcagctt gggctggagc tcgagcggca gctcgccgat ctcgtcgagg
      901 aacagcgtgc caccgtcggc ggccaggaac agcccgcgct tggcggtgtc ggcgccggtg
      961 aacgcgccgc gcaggtggcc gaacagctcg ctgtcgagca gcgtgccgac cagcgcgccg
     1021 cagttgaccg ggacgaacga acggctcgcg cggcggccgg cgaagtgcag cgcgcgggcg
     1081 accagctcct tgccggtgcc ggactcgccg gtgatcagca cggtggcgtc ggccttggcg
     1141 accttgtcga tggtctcgcg cagctgctgc atcgcgggcg agtcgccgat catcgagccg
     1201 taggccagcc gcttggtcag ctcgccgtgg gcgcggcgca gctcgtcctc gtcgagcgcg
     1261 cgccggatca ccaggcggag ctcgtcgggc gagaacggct tgccgatgta gtcgcgggcg
     1321 ccgagcttca tgccgcgcac cgcggtgtcg atcgacgggt aggcggtgat caacaggacc
     1381 gcgggcggcg ggtcgccgac cagcaggcgg ggcaacagcg acacgccgtc ctggtccggc
     1441 aggttgatgt cgcacaggat cagttcgaac cgccggcgag tcaccgcggc gagcgccccg
     1501 gcggcggagt cgaccgactc gacctcgtag ccggcgcggc ccagcacgtc gctgcacaac
     1561 gcgcggatgc cgcgctcgtc gtcgacaacg aggatggcgc tcacctcgca ggggttatca
     1621 cggccgcgcg cggggcgcca cgcgtccgtg aaaccacgtg gatccccggc gaggtgaccg
     1681 acccgtccac accgctgctt gccccaacat cgtcgcgatc tatcgggtcg ggcacgctcg
     1741 acagccggcc gtccctggtg accgggccgc gacgcgccgc cgcaacgtgg tgcactggtc
     1801 tgctcgttca gcaggggtac tgcgacgtcg agccgggcaa cgtggtgatc gatcccgacg
     1861 gagtgcccaa gatcatcgac ttcgggctgg cccggatcgc cgacggtgcc ggtcgggacc
     1921 ccggacgaca tggcgcccga ggtctggctc ggcgagccgc cgggtcgccg caccgacgtc
     1981 gactcgctcg gcgcgctgcg gttcgagcta ctcgctggcg ctccgccgtt cgccgatgtc
     2041 gagccggctg cggccgcgct cggtggatcg attagctgcc cggtggagcg tcgggagatc
     2101 acaggcccga cgcccgcgcg caggccacgc aatctatata acacctgatc gaaacgagcg
     2161 caattgattc ggcagatatt tattggcaga atatcgcgtt aatgatgaat gagtgatgct
     2221 gagctctgcg ctccatgcgc aatgaatctc gatcaaaatc accgactctc gttgagctga
     2281 tctacgccat tgcgccgttc atggcagtga acctgatagt gaacgggcat ccagagctcg
     2341 cgggatacct gcagggcaca gagcgggcgt ggcgatgtcc gggcgcggtg catcgagcaa
     2401 ctcgacatgg cagacgaaac ggccgttact ttgctgatca gactctcctc ttcgaaaagg
     2461 agagaaaatt ctcctgcccg gagcctgcgc gcgggcgtcg ggcctgtggg tggtggcgca
     2521 aaggccggtg agctgccgcg ccagactagc cgcgatggct gcggcgcgca ggtcacgggc
     2581 cgagccacca tccacaggca gcctgtgatc tcccgatgct ccacctcg
//
LOCUS       DASVOR010000835         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_124982,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000835 DASVOR010000000
VERSION     DASVOR010000835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124982"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..1389)
                     /locus_tag="VF516_16395"
     CDS             complement(73..1389)
                     /locus_tag="VF516_16395"
                     /inference="COORDINATES: protein motif:HMM:NF011656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8109315.1"
                     /translation="MPDAAPPEPTPWAKIATVVRVTGGNFLEMFDFFLFGIYAPHIAR
                     VFFPTGDEFASLMLAFGTFGAGFLMRPLGAIVLGAYVDRVGRRPGLILTLAIMAAGTI
                     LIAFVPGHAVIGIAAPLLVLIGRLLQGFSAGVELGGVSVYLAEIATPGRKGFYVSWQS
                     ASQQVAIVVAAALGYALQAGMAPATLAAWGWRVPFFVGCLIIPVIFVIRRSLQETPAF
                     LARRHRPTLREIVSSLVDHWFVVAGGTLLVVMTTVSFYLITVYTPTFGKVVLQLGAGG
                     SLLVTFCVGLSNLFWLPVMGALSDRIGRRPILVGFTLLTILTSYAAMSWLVAAPSLSR
                     MLLVELWLSVLYGSYNGAMIVTLTEVMPTEVRTTGFSLAYSLATTLGGFTPAISTWLI
                     ERLDNRAAPALWMSFSALCGLAGTAMLLSRRRGYGASSRSGWSATT"
BASE COUNT          299 a          623 c          595 g          190 t
ORIGIN      
        1 cgaggccgac acgctcgaca aggtcgaccc gcaggcgctc gccgacgacg tcgcggcggc
       61 cgccgcgctg gcctacgtgg tcgccgacca gccggagcgg ctcgacgcgc cgtagccgcg
      121 ccggcgcgac agcagcatcg ccgtcccggc caggccgcac agcgccgaga agctcatcca
      181 cagcgcgggc gccgcccggt tgtcgagccg ctcgatcagc caggtcgaga tcgccggcgt
      241 gaagccaccg agcgtggtcg ccaggctgta cgcgagcgag aacccggtgg tccggacctc
      301 ggtcggcatc acctcggtca gcgtgacgat catcgcgccg ttgtagctgc catacagcac
      361 cgacagccag agctcgacga gcagcatgcg agacaggctc ggggccgcca ccagccacga
      421 catcgccgcg tacgaggtga ggatggtcag cagcgtgaag ccgacgagga tcggccgccg
      481 gccgatccgg tccgacagcg cgcccatcac cggcagccag aacaggttcg acagaccgac
      541 acagaacgtc accagcaggc tgccgccggc gccgagctgg aggacgacct tgccgaacgt
      601 cggcgtgtag accgtgatca ggtagaagct gaccgtcgtc atgaccacga gcagcgtgcc
      661 gccggcgacc acgaaccagt ggtcgaccag cgagctcacg atctcccgca gggtcgggcg
      721 gtgacggcgg gcgaggaacg ccggggtctc ctgcagcgag cggcggatca cgaagatcac
      781 cgggatgatc aggcagccga cgaagaacgg gacccgccat ccccacgcgg cgagcgtggc
      841 cggcgccatc ccggcctgca gcgcataacc cagcgcggcg gccaccacga tcgcgacctg
      901 ctggctcgcc gactgccagc tgacgtagaa ccccttgcgc cccggcgtgg cgatctccgc
      961 caggtacacc gacacgccgc cgagctcgac gcccgccgag aacccctgca gcagccggcc
     1021 gatcaggacc agcagcggcg ccgcgatgcc gatcacggcg tggccgggga cgaacgcgat
     1081 caggatcgtg cccgcggcca tgatcgccag ggtcaggatc agtccggggc ggcggccgac
     1141 gcgatcgacg tacgcgccga gcacgatcgc accgagcggc cgcatcagga acccggcgcc
     1201 gaacgtcccg aacgccagca tcagcgaggc gaactcgtca ccggtcggga agaacacccg
     1261 cgcgatgtgc ggcgcgtaga tgccgaacag gaagaagtcg aacatctcca ggaagttgcc
     1321 gccggtgacg cgcacgacgg tcgcgatctt ggcccacggg gtcggctcgg gtggcgcggc
     1381 gtcaggcatc acgagctccc tcgggcgggc ggcgccgccg gcccaccaca cgcaaccagc
     1441 acgagcagca gccgggcgaa acccactcgg gaaatctact gctgtgcggc gccgctgaac
     1501 atcgcgatcc gcgcccggga ttgcggcaga tcccggggga tactccggac atgctcgatc
     1561 gcgtcgcccg gctgctggag cgcccgctcg tgatccgcga gccgttcgcg ccgctcgacg
     1621 cgatcgtcgt gctcggcgcg ccgctcggcc cgggtggttc gctgtcgccg gtcgcctccg
     1681 agcgcgtggt cgcggccgcc gcgctgt
//
LOCUS       DASVOR010000836         2146 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_504875,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000836 DASVOR010000000
VERSION     DASVOR010000836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504875"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF516_16400"
     CDS             complement(<1..442)
                     /locus_tag="VF516_16400"
                     /inference="COORDINATES: protein motif:HMM:NF012510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX8109316.1"
                     /translation="MFRKVLVANRGEIACRVLRTLRDLGVASVAVYHVTDRHAPHVAL
                     ADERVELRHAVPVSAYLAADQRLAACAATGADALHPGYGFLSENEDFADACEAAGIVF
                     IGPSAASMRKMGSKAGAKILMQAHGVPVVPGYTGEDQDPALLARE"
     gene            complement(408..1067)
                     /locus_tag="VF516_16405"
     CDS             complement(408..1067)
                     /locus_tag="VF516_16405"
                     /inference="COORDINATES: protein motif:HMM:NF019958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8109317.1"
                     /translation="MTQPAYSRLPRERRVADIMAAARAVFDEKGYAAASVAEIAERAG
                     VVEGSVYRYFEHKRALLIAVVERWYGELLSDFDEHLKVITGTWNRLRYLVWKHLSTIE
                     KQPMLVRLVFEELRSGPDYRRTAVFQVNRRYTRRTIDILKEGIARGEIRRDIPLGIAR
                     DMIFGGVEHHTWAFVRGEGAFSPVAAADAIVDILRRGIATAAAAPEAPHVPKSPRRKP
                     R"
     gene            complement(1064..>2146)
                     /locus_tag="VF516_16410"
     CDS             complement(1064..>2146)
                     /locus_tag="VF516_16410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109318.1"
                     /translation="ASITYPDGHRLQATLLIVGHDARAKAERVGAAILERVERINQGR
                     GLGRFRATSIEALGAERYLGAASRARDTREVVLAIGAAHDSEDALKALAREIAPAATS
                     MVQSITGFAGGRPSVQPVVRLASCLVPRPLVVPVVELRGAIIEVAGASDAPPGPGVAE
                     SAPASKKAAMDEAREGGVDGLTSTLTPNVEVDGDVRRRLHRRPQTVDASSSPASGIPT
                     GIPGASPAAARDEDSDGDAPGPPRTVPLRWLARGRSGDKGNTANIGILARSPDFVPVL
                     RAQLTADRVKQFLAHLVQGEVRRHEWPGLAGWNFVCRDALGGGGIASLRFDPQGKALA
                     QILLEMPIDVPIDVLESLAVADPEAG"
BASE COUNT          296 a          760 c          792 g          298 t
ORIGIN      
        1 cttcgcgcgc gagcagcgcc gggtcctggt cttcgccggt atagcccggc acgaccggca
       61 cgccgtgcgc ctgcatcagg atttttgcgc cggccttgga acccatcttg cgcatcgagg
      121 ccgcgctggg gccgatgaag acgatgccgg cggcttcgca cgcgtcggcg aagtcttcgt
      181 tctccgacaa gaagccgtag ccggggtgga gcgcgtcggc gccggtcgcg gcgcaggccg
      241 cgagccgctg gtcggcggcg aggtaggcgc tgaccgggac cgcgtggcgc agctcgaccc
      301 gctcgtcggc gagcgcgacg tgcggcgcgt ggcggtcggt cacgtgatag accgcgacgc
      361 tcgcgacgcc gaggtcgcgc agcgtgcgca ggacgcggca cgcgatctca ccgcggtttg
      421 cgacgaggac ttttcggaac atggggagcc tcgggggcag cagcagcggt ggcgatgccg
      481 cggcgcagga tgtcgacgat cgcatcggcg gcggcgaccg gcgagaacgc gccctcgccg
      541 cggacgaacg cccaggtgtg gtgctcgacg ccgccgaaga tcatgtcgcg cgcgatgccg
      601 agcgggatgt cgcgccggat ctcgccgcgc gcgatgccct ccttgaggat gtcgatcgtg
      661 cgccgggtgt agcggcggtt gacctggaac accgcggtgc ggcggtagtc gggacccgag
      721 cgcagctcct cgaacaccag ccgcaccagc atcggctgct tctcgatcgt cgacaggtgc
      781 ttccacacca ggtagcgcag ccggttccac gtgccggtga tgaccttgag gtgctcgtcg
      841 aagtcggaca gcagctcgcc gtaccagcgc tcgacgacgg cgatcaggag cgcgcgcttg
      901 tgctcgaagt agcggtacac gctgccctcg acgacgccgg cgcgctcggc gatctcggcg
      961 accgacgcgg cggcgtagcc cttctcgtcg aacaccgcgc gcgcggccgc catgatgtcg
     1021 gcgacccgac gctcgcgcgg tagccgcgag taggccggct gggtcatccg gcctccggat
     1081 cggcgaccgc gagcgactcc agcacatcga tcggaacatc gatcggcatc tcgagcagga
     1141 tctgggcgag cgccttgccc tgcggatcga agcgcagcga cgcgatcccg ccgccgccga
     1201 gcgcgtcgcg gcacacgaag ttccagcccg cgagcccggg ccactcgtgg cggcgcacct
     1261 cgccctgcac gaggtgggcg aggaactgct tcacgcggtc cgcggtgagc tgcgcgcgca
     1321 gcacgggcac gaaatcgggc gagcgcgcca ggatgccgat gttcgcggtg ttgcccttgt
     1381 cgccgctgcg gccgcgcgcg agccagcgca gcgggacggt ccggggcggc ccgggtgcgt
     1441 cgccgtcact gtcctcgtcc cgggcagccg cgggcgacgc gcctgggatg ccagtcggaa
     1501 tgccggatgc cggcgacgac gaagcgtcga cggtctgagg tcgacggtgg agtcgacgtc
     1561 gtacgtcgcc gtcaacctca acgttcgggg tcaacgtcga cgtcaaaccg tcaacgccac
     1621 cctcgcgtgc ctcgtccatc gctgctttct tcgacgccgg ggcggactcg gcgaccccgg
     1681 gtccgggtgg cgcatcggac gccccggcta cctcgatgat cgcgccgcgg agctcgacga
     1741 ccggcacgac cagcggtctg ggcaccaggc acgacgcgag gcgcacgacc ggttgcacgc
     1801 tcggccggcc gcccgcgaac ccggtgatcg actggaccat cgacgtcgcg gcgggggcga
     1861 tctcgcgcgc cagcgctttg agcgcgtcct cgctgtcgtg cgccgcgccg atcgcgagca
     1921 cgacctcgcg ggtatcgcgt gcgcgcgacg ccgcgcccag gtagcgctcc gcgccgagcg
     1981 cctcgatcga cgtcgcgcgg aaccggccga gcccccggcc ctggttgatc cgctcgaccc
     2041 gctcgaggat cgccgcgccg acccgctcgg ccttggcgcg ggcgtcgtgg ccgacgatca
     2101 gcagcgtggc ctgcaggcga tggccgtcgg ggtaggtgat gctcgc
//
LOCUS       DASVOR010000837         1819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_563892,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000837 DASVOR010000000
VERSION     DASVOR010000837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_563892"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VF516_16415"
     CDS             complement(<1..591)
                     /locus_tag="VF516_16415"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8109319.1"
                     /translation="MHGADDRSTAARTGGQLVVDQLAIHGVDLVFCVPGESYLAVLDA
                     LYDARDRIRLINARHEAGAANMAEAYGKLTGRPGIAMVTRGPGACHAAIGVHTAAQDS
                     TPMVLLVGQIERWAADRETFQEVDYRQMFGPLAKWVAQVDRAERVPEYLARAFHVAMS
                     GRPGPVVLALPEDMLTDAVRVADAAPAVAIQPAPDPA"
     gene            complement(578..1441)
                     /locus_tag="VF516_16420"
     CDS             complement(578..1441)
                     /locus_tag="VF516_16420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04325 family methyltransferase"
                     /protein_id="HEX8109320.1"
                     /translation="MIGTHFLSFRDDAATEGGWRGFPRRALRSIAFSAPVRARLEARY
                     ERHFATADKAHLFRGVYDSFEAAVNAAPGSKPIGYDHPGPASMYRNNLETVFLSDYPV
                     LFWLARVLGAGTRRLFDLGGHVGVRFYGFRTRLALPDTLVWQVMDVPAVVRAGRELAI
                     ERCAANLAFTDRRDDLAGADVLLATGSLQYLEKPLHDILAPIANRPPHVIINQLPMHD
                     GAAYYTLQSIGQAFCPYRIEDRRGLIAGMAALGYEQRDAWQTPEKLCPIPFHPERSVR
                     GYEGMYFHARR"
     gene            complement(1595..>1819)
                     /locus_tag="VF516_16425"
     CDS             complement(1595..>1819)
                     /locus_tag="VF516_16425"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8109321.1"
                     /translation="EFFFAPAARDAARAAAHAQSIAVAEQIQHEDVAICESVQRGLAS
                     RAYRAGRLSVRREAGEHLFHRLLARDLRGA"
BASE COUNT          263 a          684 c          637 g          235 t
ORIGIN      
        1 ggccggatcg ggggcgggct ggatcgcgac cgccggcgcg gcatcggcca cacggaccgc
       61 gtcggtcagc atgtcctcgg ggagcgccag cacgaccggc cccggccgcc ccgacatcgc
      121 gacgtggaac gcgcgcgcca ggtactccgg cacgcgctcg gcgcgatcga cctgcgcgac
      181 ccacttggcg agcggcccga acatctgccg gtagtcgacc tcctggaacg tctcgcggtc
      241 ggccgcccag cgctcgatct ggccgaccag cagcaccatc ggcgtcgagt cctgcgcggc
      301 ggtgtgcacg ccgatcgccg cgtgacacgc gcccggcccg cgcgtgacca tcgcgatccc
      361 cggccgcccg gtgagcttgc cgtacgcctc ggccatgttg gccgcgcccg cctcgtgccg
      421 cgcgttgatc agccggatcc ggtcgcgcgc gtcgtacagc gcatcgagca ccgcgaggta
      481 gctctcgccg gggacacaga acaccaggtc gacgccgtgg atcgcgagct gatccacgac
      541 cagctgcccg ccggtccgcg cggccgtgga gcgatcgtca gcgccgtgca tgaaagtaca
      601 tgccctcgta gccgcgcacc gagcgctcgg gatggaacgg gatcgggcac agcttctccg
      661 gggtctgcca cgcgtcgcgc tgctcgtagc cgagcgccgc catgcccgcg atcaggccgc
      721 ggcggtcctc gatgcgatac gggcagaacg cctgtccgat gctctgcagc gtgtaatacg
      781 ccgcgccgtc gtgcatcggc agctggttga tgatcacgtg cggcggccgg ttcgcgatcg
      841 gcgcgaggat gtcgtggagc ggcttctcga gatactgcag cgatccggtc gcgagcagga
      901 catcggcgcc cgccaggtcg tcgcgccggt cggtgaacgc caggttcgcc gcgcaccgct
      961 cgatcgcgag ctcgcggccg gcgcgcacca ccgcggggac gtccatgacc tgccagacca
     1021 gcgtgtcggg cagcgccagc cgggtgcgaa agccgtagaa ccgcacgccg acatggcccc
     1081 cgagatcgaa cagccgccgc gtcccggcgc cgagcacgcg ggccagccag aacagcaccg
     1141 gatagtcgga caggaacacg gtctcgaggt tgttgcggta catcgacgcc gggcccggat
     1201 gatcgtagcc gatcggcttg gagccgggcg ccgcgttcac cgcggcctcg aagctatcgt
     1261 acacaccgcg aaacaggtgc gccttgtccg cggtcgcgaa gtggcgctcg tagcgcgcct
     1321 ccagccgcgc gcggaccggc gcgctgaacg cgatcgagcg cagcgcgcgc cgcgggaacc
     1381 cgcgccagcc gccctcggtg gccgcgtcat cgcggaacga caggaaatgc gtgccgatca
     1441 tggtggaccc ttggtgtccc agaatgggca tcccagaatg ccccacgcgg cccgctcgcg
     1501 cacgcgacca cggccgtgat tgccccggcg atgcgcgccc tccggcgcct ggacgccgat
     1561 gtcgtaccgc ctccgacccg ccgttcgccg tgcgctatgc gccgcgcagg tcgcgcgcca
     1621 gcagccgatg gaacaggtgc tcgcccgcct cgcggcgcac cgacagccgt cccgcgcgat
     1681 acgcgcgcga cgccaggccg cgctgcacgc tctcgcagat cgcgacgtcc tcgtgctgga
     1741 tctgctcggc caccgcgatg ctctgcgcgt gggcggccgc gcgcgccgcg tcccgggcgg
     1801 ccggcgcgaa gaagaactc
//
LOCUS       DASVOR010000838         4761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_212893,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000838 DASVOR010000000
VERSION     DASVOR010000838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212893"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VF516_16430"
     CDS             <1..192
                     /locus_tag="VF516_16430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109322.1"
                     /translation="RQLADHGRPVQYEMRIPPATSASTASRRRLFVLQPSTSAARANR
                     VITADAEAGDPRPGLSQDG"
     gene            complement(644..1498)
                     /locus_tag="VF516_16435"
     CDS             complement(644..1498)
                     /locus_tag="VF516_16435"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8109323.1"
                     /translation="MTDPNPPADAPIYRGMNRAALDAAYNNSAAVADSDRWLEQWRRR
                     SAEVRGAPGARLDIAYGPKERNRFDYFPSGRPGAPLFVFLHGGYWQRNAKDLFAFIAE
                     GPRQRGIDVAVVGYTLAPDARLSEIVREIADALSFLHGNAAVFGFDRRALCVGGWSAG
                     GHLAAMSCGHPAVRSALAISGIFDLEPIALNYINDRLRLDAAEVATLSPAHTLRPGWA
                     PLALAVGGDELPELQRQSTSFHDIADSLGVAMALHVLPGHHHYSILDELARPDGALTG
                     ELLRLVAA"
     gene            complement(1541..2365)
                     /locus_tag="VF516_16440"
     CDS             complement(1541..2365)
                     /locus_tag="VF516_16440"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8109324.1"
                     /translation="MPELQPSAPPEHAAGILRVAGVDKTFPAAAAPVEALRDIDLSVR
                     DGEFVCLLGASGCGKSTLLRIIAGFERATRGSVSVRGIAVDRPGPDRGMVFQDYALFP
                     WLTVRQNIEFGPRQRGVPPAELARTTARFLAMVGLTASADRYPYQLSGGMKQRVAIAR
                     VLANHADILLMDEPFGALDELIRAKLQDDLIEIWQSTKLTILFVTHSVEEAVLLADRI
                     LVMTAGPGRIDGEVHVDLPRPRDVSSPEFNAVRRDVTQRLTSHLVRSPAAAAGSEP"
     gene            complement(2358..3224)
                     /locus_tag="VF516_16445"
     CDS             complement(2358..3224)
                     /locus_tag="VF516_16445"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8109325.1"
                     /translation="MHPPTSAAETVDRAIAPASVSPAPGRSARRLVREVALGAVVPLG
                     LLVFWHAMVRWTGTRLIPPPREVAVTMWDFAVGGIYDDAYSASLPVHLWKSVQRVYGG
                     FLCAALAGVPLGLAIGRLPVVRAMLDPTLSLLRPIPVTAWLPLSMIFFGLGPKSAVFL
                     VFLGAFYPILLNTILGVKSVDPRLFEAAEMLGCRGPRLFRAVVLPAALPSIFTGLRLG
                     AGVAWLMIVVGEMTGVPEGLGAVIMDGRTLSRTDLVITGMILIGVAGFVSDRILLRLN
                     NHFLRWSPQHHA"
     gene            complement(3226..4182)
                     /locus_tag="VF516_16450"
     CDS             complement(3226..4182)
                     /locus_tag="VF516_16450"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrtA/SsuA/CpmA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8109326.1"
                     /translation="MHRTSRREFLRLAAATAAFATGSRRAWADHRVIKVGTLKLIHGI
                     TPYFYDRFATADLTFEVVPFESPTDGKNAVVTGTVDFGIYGIAAATLGAANGEPVVVI
                     AAACNRGMAIVARQDAPIAAIKDLRGKRVGIWPGSTQEVVLLDRLSAEGLTIKDISPV
                     RVSFSDMAPALARGDIDAYVGAEPGPGISLASGAGKIVEYPYSTPIGPLNMVLSTRRE
                     LVQKDPALIRALLQIHRKASEYAMSHRDAFVAMATQKLGQHKPSIEKAAPNVELTWEI
                     DAAFLQRARYYGTQMLAKKQIRQLPDYNTFIDPSFVKAIAGP"
     gene            4314..>4761
                     /locus_tag="VF516_16455"
     CDS             4314..>4761
                     /locus_tag="VF516_16455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109327.1"
                     /translation="MKTALVLVAVLSAAAPASAFTLEQVMSAPFCSGLVVAARAPRIA
                     WTVNLRGERNVWVAEAPAWKPHAVTHYRGDTGQPLDALAISPDGKLVVFVRGGEGEHT
                     PNPTSLAQPPKRQVIAAAADGSGEPRVLGDGGDGLQLSPDGSRVAWL"
BASE COUNT          720 a         1646 c         1741 g          654 t
ORIGIN      
        1 cgccagctcg ccgatcacgg gcgcccggtc cagtacgaga tgcggatccc gcccgcgacg
       61 tccgcgtcca ccgcgtcgcg ccgccggctg ttcgtgctgc agccgagcac cagcgccgcg
      121 cgcgccaacc gggtgatcac ggcggacgcc gaggccggcg acccgcgccc ggggctgtcg
      181 caagatgggt agccagcgca gcaggcgcgc agtaggttgg atggatcggc ttcgacggtg
      241 aacgagtact acatggaacc gtacaagcag atcagcggtg gcaagtgacc tcatgcgcgc
      301 cgcccgggcc accatcatcg cgctggccat ggccggcgtc gcgtgccagc cggccccgat
      361 cgcgccaccg aagggagcag gaccgaaaac agcagacatg gcatgcgaag gaacgtttct
      421 ctcatgacat ccgcttgccg cgcgctggtg ctggctcgag gccgtgccga aggggaaccc
      481 acttggctgt gttgtggccg acctggtggc cgaggttacc tgttacccag cgccccgacc
      541 agccgcgtct aaccctaggt tggattctcc gtcgtcatcc agacgacgag ctgttccacg
      601 tcgatggcga cggtaggacg cccggtccgg cggcgcgccg agcctaggcc gcgacgaggc
      661 ggagcagctc gccggtcagc gcgccgtcgg ggcgggcgag ctcgtcgagg atcgagtagt
      721 ggtgatggcc cggcagcacg tgcagcgcca tcgccacgcc cagcgagtcg gcgatgtcgt
      781 ggaagctcgt cgactggcgc tggagctccg gcagctcgtc gccgccgacc gcgagggcca
      841 gcggcgccca cccggggcgc agcgtgtgcg ccgggctcaa cgtggcgacc tccgcggcgt
      901 cgagccgcag ccggtcgttg atgtagttga gcgcgatcgg ctccagatcg aagatgccgc
      961 tgatcgccag cgcgcttcgc acggcgggat gaccgcacga catggcggcg aggtggccgc
     1021 ccgccgacca gccgcccacg cacagggcgc gccggtcgaa gccgaacacg gccgcgttgc
     1081 cgtggaggaa cgacagcgcg tcggcgatct cgcggacgat ctcggacagc cgcgcgtccg
     1141 gggccagggt gtagccgacg acggcgacgt cgatcccgcg ctggcgaggc ccctcggcga
     1201 tgaacgcgaa caggtccttc gcgttgcgct gccagtagcc cccgtgaagg aacacgaaca
     1261 gcggggcgcc ggggcgtccc gacgggaagt agtcgaagcg gttccgctcc ttcgggccgt
     1321 aggcgatgtc gaggcgagcg cccggggctc cgcggacctc cgcgctgcgg cggcgccact
     1381 gctcgagcca gcggtcgctg tcggcgacgg cggcgctgtt gttgtaggcg gcgtcgaggg
     1441 ccgcgcggtt catcccgcga tagatcggcg cgtcggcagg tgggttgggg tcggtcatgt
     1501 caggcatggc atgggtctcc gggttccgac agctccgaca tcagggctcc gagcctgctg
     1561 cggccgcggg cgagcggacg aggtggctgg tcagtcgctg ggtcacgtcg cggcgcacgg
     1621 cgttgaactc cggcgacgag acgtcgcggg gccgcggcag atcgacgtgg acctcgccgt
     1681 cgatccggcc cgggccggcg gtcatgacga ggatccgatc ggcgagcagg acggcctcct
     1741 ccacggagtg ggtcacgaac aggatggtca gcttggtgct ctgccagatc tcgatcaggt
     1801 cgtcctggag cttggcgcgg atcagctcgt cgagcgcgcc gaagggctcg tccatcagca
     1861 ggatgtcggc gtggttcgcg agcacgcggg cgatcgcgac gcgctgcttc atgccgccgg
     1921 acagctggta cggatagcga tcggcggacg cggtgagccc gaccatcgcc aggaaccggg
     1981 cggtggtccg tgcgagctct gccgggggga cgccgcgctg gcgcgggccg aactcgatgt
     2041 tctggcgcac ggtcagccag gggaacagcg cgtagtcctg gaacaccatg ccgcggtcgg
     2101 ggcccgggcg gtcgacggcg atgccgcgga ccgagaccga tccgcgcgtg gctcgctcga
     2161 agccggcgat gatgcgcagc agggtggact tgccgcaccc cgacgcgccg agcaggcaca
     2221 cgaactcgcc gtcccggacc gagagatcga tgtcgcgcaa ggcctcgacc ggcgcggccg
     2281 ccgccggaaa cgtcttgtcg acgccggcga cccgcaggat tcccgcggcg tgctccggcg
     2341 gagcgctggg ctgcagctca ggcatgatgc tgcgggctcc agcgcaggaa gtggttgttg
     2401 agccggagca ggatccggtc ggacacgaag ccggccacgc cgatgaggat catgcccgtg
     2461 atcacgagat cggtgcgcga cagcgtgcgg ccgtccatga tgaccgcgcc gaggccttcc
     2521 gggacgccgg tcatctctcc gacgacgatc atgagccacg cgaccccggc gcccagccgc
     2581 aggccggtga agatgctcgg cagcgccgcc gggagcacca ccgcgcggaa cagccgcggc
     2641 ccgcggcagc cgagcatctc ggcggcctcg aacagccgcg ggtcgaccga cttcacgccg
     2701 aggatggtgt tgagcaggat cgggtagaac gcgccgagga agaccaggaa cacggccgat
     2761 ttcggcccga gcccgaagaa gatcatcgac agcggcagcc acgcggtgac cgggatcggg
     2821 cggagcagcg acagcgtcgg atcgagcatc gcgcggacca ccggcagccg gccgatcgcg
     2881 aggcccaggg ggacgccggc cagcgccgcg cacaggaatc cgccgtagac ccgctgcacc
     2941 gacttccaca ggtgcacggg gaggctcgcg ctgtaggcat cgtcgtagat cccgccgacc
     3001 gcgaagtccc acatcgtcac cgcgacctcg cgcggcggcg ggatcaaccg cgttccggtc
     3061 cagcgcacca tcgcgtgcca gaacacgagc agaccgagcg gcaccacggc gcccagcgcg
     3121 acctcgcgca cgaggcgtcg cgcgctgcgc ccgggcgccg ggctcacgct cgcaggggcg
     3181 atcgcgcgat cgacggtctc ggcggccgac gtgggagggt gcatctcagg gccccgcgat
     3241 ggccttcacg aagctcggat cgatgaacgt gttgtagtcc ggcagctggc ggatctgctt
     3301 cttggccagc atctgcgtgc cgtagtagcg ggcgcgctgg aggaacgccg cgtcgatctc
     3361 ccaggtcagc tcgacgttgg gcgcggcctt ctcgatcgac ggcttgtgct ggccgagctt
     3421 ctgggtcgcc atcgccacga acgcgtcgcg gtggctcatc gcgtactcgc tcgccttgcg
     3481 gtggatctgg agcagcgcgc ggatcagcgc gggatccttc tgcaccagct cgcgccgcgt
     3541 gctcagcacc atgttgagcg ggccgatcgg tgtcgagtac ggatactcga cgatcttgcc
     3601 ggcgccgctg gcgaggctga tgcccggccc gggctcggcc ccgacgtacg cgtcgatgtc
     3661 gccgcgcgcg agcgccggcg ccatgtcgct gaacgacacg cggaccggtg agatgtcctt
     3721 gatcgtcagg ccctcggcgg acagccggtc gagcagcacg acctcctggg tcgagcccgg
     3781 ccagatcccg acccgcttgc cccggaggtc cttgatcgcc gcgatcggcg cgtcctggcg
     3841 cgcgacgatc gccatgccgc ggttgcaggc agcggcgatc acgacgaccg gctcgccgtt
     3901 ggccgccccg agcgtcgcgg cggcgatccc gtagatgccg aagtcgacgg tgccggtgac
     3961 cacggcgttc ttgccgtcgg tgggactctc gaacggcacc acctcgaacg tcaggtccgc
     4021 cgtcgcgaac cggtcataga agtacggcgt gatgccgtgg atgagtttca gcgtcccgac
     4081 cttgatgacg cggtgatccg cccatgcgcg ccggctgccg gttgcaaacg cggcggtcgc
     4141 cgccgccaga cgcaggaact ctcgacgaga cgttcggtgc atgactcccc tcgtgatcgc
     4201 aatctccgca ggcaaggcgc tcgccgccat gcctgcccac gcatcgcggg cgacagcatc
     4261 gcacaccggg tatccccatg tcttgcctcg tcgctgtgga ttacagtggc gccatgaaaa
     4321 ccgccctcgt gctggtcgcc gtcctgtccg ccgccgcgcc ggcgtcggcg ttcacgctcg
     4381 aacaggtcat gtccgcgccg ttctgcagcg ggctggtcgt tgccgcgcgg gcgccgcgca
     4441 tcgcgtggac cgtgaacctg cgcggcgagc gcaacgtgtg ggtcgccgag gcgccggcgt
     4501 ggaagccgca cgcggtgacc cattaccgcg gcgacaccgg acagccgctc gacgcgctcg
     4561 cgatcagccc cgacggcaag ctcgtggtgt tcgtccgcgg cggcgaaggc gagcacacgc
     4621 cgaacccgac gagcctggcc cagccgccga agcgccaggt gatcgcggcg gccgccgacg
     4681 gcagcggcga gccgcgcgtg ctcggcgacg gcggcgacgg gctccagctg tcgcccgacg
     4741 gctcgagggt cgcgtggctg g
//
LOCUS       DASVOR010000839         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_416816,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000839 DASVOR010000000
VERSION     DASVOR010000839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416816"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VF516_16460"
     CDS             <1..304
                     /locus_tag="VF516_16460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109328.1"
                     /translation="RLEFVDRRPDGLRQNQRLTARILIEERPNVLKVERGPFLETGGG
                     NTAYFVVDGIAERRPIRTGAISLDAVEIVSGASPGDRIVVTGADSFGTAQRVRIAE"
     gene            355..1071
                     /locus_tag="VF516_16465"
     CDS             355..1071
                     /locus_tag="VF516_16465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8109329.1"
                     /translation="MKDVSKVYRTELVETHALRDLTLEVGDGEFVAVTGPSGSGKTTF
                     LNIAGLLEELTGGEYLLDGVEVKQLDDRARSRLRNEKIGFVFQSFNLIPDLDLFDNVD
                     VPLRYRGLTRGERKKRIEAALERVGLASRVRHFPAELSGGQQQRVAIARAIAGDPKLL
                     LADEPTGNLDSQMAREVMQLLEELNAQGTVVVIVTHDPEIAARAQRNVHIVDGVASDL
                     ERGPSLVRSARAAAQPSAQG"
     gene            1077..2378
                     /locus_tag="VF516_16470"
     CDS             1077..2378
                     /locus_tag="VF516_16470"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8109330.1"
                     /translation="MFAYYLDLARRSFRRNVALTALMIIAIAFGVGASMTTLTVLHVL
                     SVDPIPGKSQQLYYVQLDPVRALGYMPGEEPMEQMTRLDSEALLRAARADRQALMTSG
                     RAAVEPQRPGLAPFFVDGRWTSADLFAMFQVPFLAGAAWSADDDTRAGRVAVVTRALA
                     DKLYGTTDAVGRTIRVNGHDLRVIGVIADWRPTPHFYDLNNDVYGAGEQLFVPFSTSA
                     DLKLQRSGSLDCWDEVAGDPLAVGAPCVWTQLWVELGSRDKAIAYRDFLVSYSLEQMR
                     TGRFQRPPNVRLRTVTEWLDFKGVVPNDVRLQTWIAFGFLLVCLINTVGLLLTKFLRR
                     SAEIGVRRALGASKRSIFVQLLVEAGAIGLVGGTLGLGLAWLGLWAVRHQPADYADLA
                     RLDLSMLGSTFALAILASLLAGLLPAWRGCQVTPAIQLKSH"
     gene            2386..3600
                     /locus_tag="VF516_16475"
     CDS             2386..3600
                     /locus_tag="VF516_16475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8109331.1"
                     /translation="MHFFPILSTLRRHRTAAALIVLEIALTCAIVCNAIFLIRDRLSR
                     MDRPSGVAEAELVRVQLTGIGTQSDQHALTAQDLAALRAIPGVKFAAATNMVPFGLSS
                     WNNTVSTKFDDVNGTGAGVYIGSPDLLETLGVELVAGRDFTPDEYIDFDEGNPGEMRV
                     SSVIITRDLADYWFPGKSPIGQAFYATGKEPQTIVGVIDRLHRPNVNRSGRESFAVVL
                     PLRAPSASGGDYLLRVDPARKGDVLAAVEPTLNKVSPSRIILKRQTFAEIRDNYFKAD
                     RAMAYLLIGVSLALLIITALGVVGLASFWVQQRTRQIGVRRALGATRGDILRYFQLEN
                     FILASAGIVLGMALAYAINLWLMHKYEVARLPVEFLPVGAGLLWILGQIAVLGPAMRA
                     ATIPPAIATRTV"
     gene            3634..>4742
                     /locus_tag="VF516_16480"
     CDS             3634..>4742
                     /locus_tag="VF516_16480"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8109332.1"
                     /translation="MPTVLVIDDNPAVATALEVLFSLRDLRTLSADSPARALAILDRQ
                     PVDVVIQDMNFRADTTSGEDGAALFRVLRARFPDLPVILLTAWTQLESAVELVKAGAA
                     DYLAKPWDDNTLLTAVHNLIELSETRRELARRRSDETRRKTELEQRYDLRGAVFADPA
                     MERVIALACQVARADVPVLITGPNGAGKEKIAEIVQANSAVPDGPFVTLNCGALPSEL
                     IEAELFGAEIGAYTGAVRAREGKFEAADGGTLFLDEIGNLSANGQMKLLRVLETGRFE
                     RLGGSRERQVKVRVISATNADLHAMIRAGTFREDLYYRLNTIEIRVPPLADRVDDILP
                     LARAFLAAGLAPGKRLSADAREALRHHSWPGNVREL"
BASE COUNT          681 a         1768 c         1606 g          687 t
ORIGIN      
        1 ccggctggag ttcgtcgacc ggcggcccga cgggctgcgc cagaaccagc ggctgaccgc
       61 gcggatcctg atcgaggagc ggcccaacgt gctcaaggtc gagcgcggcc cgttcctcga
      121 gaccggcggc ggcaacaccg catacttcgt cgtggacggc atcgccgagc gccggccgat
      181 ccggaccggg gcgatcagtc tggacgcggt cgagatcgtg tcgggcgcca gccccggcga
      241 ccggatcgtg gtcaccggcg cggacagctt cggaaccgcc cagcgggtgc ggatcgcgga
      301 gtaggaatcc cagcccaccg agcctgcagc gaggaaccca tgcccatgct tcacatgaaa
      361 gacgtctcca aggtgtaccg gaccgagctg gtcgagaccc acgcgctgcg cgacctgacg
      421 ctggaggtcg gcgacggcga gttcgtcgcc gtgaccggtc cgtcgggctc gggcaagacc
      481 acgttcctca acatcgccgg cctgctcgag gagctgaccg gcggcgagta cctgctcgac
      541 ggcgtcgagg tcaagcagct cgacgaccgc gcgcggtcgc ggctgcgcaa cgagaagatc
      601 gggttcgtgt tccagagctt caacctgatc cccgacctcg acctgttcga caacgtcgac
      661 gtcccgctgc gctaccgcgg gctgacccgg ggcgagcgca agaagcggat cgaggccgcg
      721 ctcgagcggg tcggcctggc gtcgcgcgtc cggcactttc ccgccgagct atccggcggc
      781 cagcagcagc gggtcgcgat cgcgcgggcg atcgccggcg accccaagct gctgctcgcc
      841 gacgagccga ccggcaacct cgactcgcag atggcgcgcg aggtcatgca gctgctcgag
      901 gagctcaacg cgcagggcac cgtggtcgtg atcgtcaccc acgaccccga gatcgccgcg
      961 cgcgcccagc gcaacgtcca catcgtcgac ggcgtcgcca gcgacctcga gcgcggcccg
     1021 tcgctggtcc gctccgcccg cgccgcggcg cagccgagcg cccagggata gccgccatgt
     1081 tcgcgtacta cctcgacctc gcgcgccgca gcttccggcg caacgtcgcg ctgaccgcgc
     1141 tgatgatcat cgcgatcgcg ttcggcgtcg gcgccagcat gaccacgctc accgtgctcc
     1201 acgtgctgtc ggtcgacccg atcccgggca agagccagca actgtactac gtgcagctcg
     1261 atccggtccg cgcgctcggc tacatgcccg gcgaggagcc gatggagcag atgacccggc
     1321 tcgactccga ggcgctgctg cgcgcggcca gggccgaccg ccaggccctg atgaccagcg
     1381 gccgggccgc ggtcgagccc cagcgccccg ggctggcgcc gttcttcgtc gacgggcggt
     1441 ggacctcggc cgacctcttc gcgatgttcc aggtcccgtt cctcgccggc gcagcgtgga
     1501 gcgcggacga cgacacccgc gcgggccggg tcgccgtggt cacgcgcgcg ctcgccgaca
     1561 agctgtacgg caccaccgat gcggtcggcc gcacgatccg ggtcaatggc cacgacctcc
     1621 gggtgatcgg cgtgatcgcc gactggcggc cgaccccgca cttctacgac ctcaacaacg
     1681 atgtctacgg cgcgggcgag cagctgttcg tgccgttctc gacctcggcc gacctcaagc
     1741 tgcaacgcag cggcagcctg gactgctggg acgaggtcgc cggcgatccg ctcgcggtcg
     1801 gcgccccgtg cgtgtggacc cagctctggg tcgagctcgg cagccgggac aaggccatcg
     1861 cgtaccgcga cttcctggtc agctactcgc tcgagcagat gcggaccggg cggttccagc
     1921 ggccgcccaa cgtccggctg cgcaccgtga ccgagtggct cgacttcaag ggcgtggtgc
     1981 cgaacgacgt ccggctgcag acctggatcg cgttcggctt cctgctggtg tgcctgatca
     2041 acaccgtggg cctgttgctc accaagttcc tgcggcggtc ggccgagatc ggcgtgcggc
     2101 gcgcgctcgg tgcgtcgaag cggtcgatct tcgtccagct gctcgtcgag gccggcgcga
     2161 tcgggctggt cggcggcacg ctcggcctcg gcctggcgtg gctcgggctg tgggcggtgc
     2221 gccaccagcc ggccgactac gccgacctcg cgcggctcga cctgtcgatg ctcggctcga
     2281 cgttcgcgct cgcgatcctc gccagcctgc tcgccggtct gctccccgcg tggcgcggct
     2341 gccaggtcac gcctgccatc cagctcaagt cgcactgagg gccccatgca cttcttcccg
     2401 atcctgtcca cgctccgccg ccaccgcacc gccgccgcgc tgatcgtgct cgagatcgcg
     2461 ctgacctgcg cgatcgtgtg caacgcgatc ttcctgatcc gcgatcgcct gtcgcgcatg
     2521 gaccggccga gcggcgtcgc cgaggccgag ctggtccgcg tccagctcac cggcatcggc
     2581 acgcagagcg accagcacgc gctgaccgcg caggacctcg ccgcgctgcg cgcgatcccc
     2641 ggcgtcaagt tcgccgccgc caccaacatg gtcccgttcg gcctgtcgtc gtggaacaac
     2701 accgtgtcga ccaagttcga cgacgtcaac ggcaccggcg ccggcgtcta catcggctcc
     2761 ccggacctgc tcgagaccct cggcgtcgag ctcgtcgccg gccgcgactt cacgcccgac
     2821 gagtacatcg acttcgacga ggggaacccc ggcgagatgc gggtgtcgtc ggtgatcatc
     2881 acgcgcgatc tcgccgacta ctggttccca ggcaagagcc cgatcggcca ggcgttctat
     2941 gccaccggca aggagccgca gaccatcgtc ggggtgatcg accggctgca ccggccgaac
     3001 gtgaaccgct ccgggcgcga gagctttgcc gtggtgctgc cgctccgggc gccctcggcc
     3061 tcgggcggcg actacctgct gcgggtcgat cccgcccgca agggcgacgt gctcgccgcc
     3121 gtcgagccga ccctcaacaa ggtcagcccg agccgcatca tcctgaagcg tcagacgttc
     3181 gccgagatcc gcgacaacta cttcaaggcg gaccgcgcga tggcctacct gctgatcgga
     3241 gtctcgctcg cgctcctgat catcacggcg ctcggcgtcg tcgggctcgc cagcttctgg
     3301 gtccagcagc ggacccgcca gatcggcgtg cgccgcgcgc tcggtgcgac ccgcggcgat
     3361 atcctgcgct acttccagct cgagaacttc atcctggcga gcgccggcat cgtgctcggc
     3421 atggcgctgg cctacgcgat caacctctgg ctcatgcaca agtacgaggt cgcgcggcta
     3481 cccgtcgagt tcctgccggt cggcgcgggc cttctgtgga tcctcggcca gatcgcggtg
     3541 ctcggcccgg cgatgcgcgc cgcgacgatt ccgcctgcga tcgcgacccg caccgtgtag
     3601 cctgggtctg gacctggacc tggtacggaa gcgatgccca ccgtcctcgt gatcgacgac
     3661 aacccggcgg tggcgaccgc gctcgaggtg ctgttctcgc tgcgcgacct gcgcacgctc
     3721 agcgccgact cgccggcccg ggcgctggcg atcctcgacc gccagccggt cgacgtcgtg
     3781 atccaggaca tgaacttccg cgccgacacc acctcgggcg aggacggcgc cgcgctgttc
     3841 cgcgtgctgc gggcgcggtt ccccgatctg ccggtgatcc tgctcaccgc gtggacccag
     3901 ctcgagtcgg cggtcgagct ggtaaaggcc ggcgccgccg actacctcgc caagccgtgg
     3961 gacgacaaca cgctgctcac cgccgtccac aacctgatcg agctgtccga gacccggcgc
     4021 gagctcgcgc ggcggcgcag cgacgagacc cggcgcaaga ccgagctcga gcagcgctac
     4081 gacctgcgcg gcgcggtgtt cgccgacccg gcgatggagc gcgtgatcgc gctggcctgc
     4141 caggtcgcgc gcgccgacgt gccggtcctg atcaccggcc ccaacggcgc cggcaaggag
     4201 aagatcgccg agatcgtcca ggccaactcc gcggtccccg acggcccgtt cgtcacgctc
     4261 aactgcggcg cgctgccctc ggagctgatc gaggccgagc tgttcggcgc cgagatcggc
     4321 gcgtacaccg gggccgtgcg cgcgcgcgag ggcaagttcg aggccgccga cggcggcacg
     4381 ctgttcctcg acgagatcgg caacctgtcg gccaacggcc agatgaagct gctccgcgtg
     4441 ctggagaccg gccggttcga gcgactcggc ggaagccgcg agcgccaggt caaggtccgc
     4501 gtgatcagcg cgaccaacgc cgacctccac gcgatgatcc gcgccggcac gttccgcgag
     4561 gacctgtatt accggctcaa cacgatcgag atccgcgtcc cgccgctcgc cgatcgggtc
     4621 gacgacatcc tgccgctggc ccgggcgttc ctggccgcgg gcctcgcccc cggcaagcgg
     4681 ctgtccgccg acgcgcgcga ggcgctgcgc caccacagct ggccgggcaa cgtgcgcgag
     4741 ct
//
LOCUS       DASVOR010000840         3221 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_358990,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000840 DASVOR010000000
VERSION     DASVOR010000840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358990"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2040)
                     /locus_tag="VF516_16485"
     CDS             complement(<1..2040)
                     /locus_tag="VF516_16485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger-like domain-containing protein"
                     /protein_id="HEX8109333.1"
                     /translation="MRTRAWLSVRRYRRSPRYDVGAGALVLLMVVVAAAGVYAVMLAA
                     VAFVAWLLRIATRRRPEASAEPPLQIPTSAPYRAVLPVDPAVFVDDAEAKAVAKRVFE
                     QWVSGLPRAPARSVDLVRAVDIRTRQVGRLVTEFEGRRVVECCEPAPGREPPRPPPFV
                     RQSIDPWNPPHDLPRVSRFIAACWTCGASGRVECASCRGTARRPCTGCDGSGKYYGQA
                     ANGTHRLLNCKTCRGKGDVRCADCTCGKVDCETCHQAKKLACWLEIRSDLRQDVQFAP
                     EDAATLPFVWCRRDAPVADDQLTLDARIVDKVTRARPLAATELPVTVPPEWRSKHGPS
                     LQAHVEPGERVRSQSFTLLSVPFASVTYAVLGEQHAVTLEGLRMLAPPVTAPAAEPFA
                     RRARMLGRLALAFAALPVAIGAIYAARGEYFTSGRATGLVAGVAIAAAATAALAYAAL
                     WNVTLGRRRARTWAMAAIAPVTLASILAAVAEPRLDRARDLLDAGGLDEATAELRALN
                     VPEEDAAWADLHLRHSLEVTTCATATAELRPIPTEQPQRRQAQAHADQLAIAETEAAI
                     RAHDPDAAAIALGCTSEPGRTSPTALALRAQIADLQAGRCLQRKDWACALDRAASGSD
                     PATLRADALAAINAEANAQIAAAAREPSLDQRIADEQAALDLWQSYLLDPQYPDPP"
     gene            2163..2921
                     /locus_tag="VF516_16490"
     CDS             2163..2921
                     /locus_tag="VF516_16490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109334.1"
                     /translation="MKVAAVVVGTDDERFRRVDANRERMQLAERIAGFTKSTADVILL
                     PAGFLAAKADREVRRHAESLAEVFGKQVLLAGVDESDETRSASKDRRFGKSKDATGNG
                     EGHPFWVFASRNGKLIGGPWQQRSAFSGDAVSDPAPRCVSVGESTIGVLICGELYNRA
                     IADSLAEAKPDLVVDLAHLSMKRFTRSLHRVAEAAECPVLHVQHVALGSRTAAKWTAG
                     TQEARGDRAVDWASYNEPDLASDGLWAEVKLWKV"
BASE COUNT          506 a         1009 c         1214 g          492 t
ORIGIN      
        1 gggcggatcc gggtactgcg gatccaggag gtaactttgc cacagatcga gcgcagcctg
       61 ctcgtcggcg atgcgctgat ccaggctcgg ttcgcgggct gcagcggcga tctgcgcgtt
      121 cgcctcagcg ttgatcgccg cgagcgcgtc ggcgcggagc gtggccggat cgctgccgga
      181 ggcggcgcgg tcgagggcgc atgcccagtc cttgcgctga aggcagcgtc cggcctggag
      241 gtcggcgatc tgggcgcgca gtgcgagcgc agtggggctg gtgcggccag gctcgctcgt
      301 gcagccgagg gcgatcgcgg cggcatcggg atcgtgtgcg cgaatggccg cctcggtctc
      361 ggcgatggcg agctggtcgg catgcgcctg ggcttgtctg cgctggggct gctccgttgg
      421 aatgggccgc agctctgcgg tggcagttgc gcaggtggtg acttcgagcg agtggcggag
      481 gtggaggtcg gcccatgcag catcctcctc tggtacattc agcgcgcgta gctcggcggt
      541 ggcctcgtcg agcccgccgg catcgaggag gtcgcgggcc cgatcgaggc gcggttcggc
      601 gacggcagcc aggatgcttg cgagcgtgac cggcgcgatg gcagccatgg cccatgtccg
      661 cgcgcggcgg cggccgagtg tgacgttcca cagcgctgcg taggcgagcg cggcggtcgc
      721 cgcggcggcg atggctacgc ctgcgaccag tccggtggcg cgaccgctgg tgaagtactc
      781 gccacgggcg gcgtagatcg caccgatcgc aacgggcagt gcggcgaacg cgagcgcgag
      841 gcgtccgagc atgcgagcgc gtcgcgcgaa cggctcggcg gccggggcgg tcaccggcgg
      901 cgcgagcata cggagcccct ccagtgtgac ggcatgctgc tccccgagaa cagcgtacgt
      961 gacggaagcg aacgggacgg acagcaaggt gaaagactgt gaccgcacgc gctcgccggg
     1021 ctcgacgtgc gcttgcaggc tcgggccgtg cttgctgcgc cattccgggg ggacggtcac
     1081 ggggagctcg gtggcagcga ggggccgcgc ccgagtgacc ttgtcgacga tgcgcgcgtc
     1141 gagagtgagc tggtcatcgg cgaccggcgc gtcgcgccga caccatacga acggcagggt
     1201 ggccgcatcc tcgggcgcga actggacgtc ctggcgcagg tcactcctga tctccagcca
     1261 gcatgcgagc ttcttggctt gatggcaggt ctcgcagtcg accttgccac acgtacagtc
     1321 cgcgcagcga acatcgccct tgcctcggca ggtcttgcag ttcagcaaac gatgggtccc
     1381 gttggccgcc tggccgtagt acttgccgga gccgtcgcag ccggtacatg ggcggcgcgc
     1441 ggtgccgcgg cacgaagcgc actcgacgcg gccgctcgcc ccgcaggtcc agcacgcggc
     1501 gatgaagcgg ctaacgcgcg gtaagtcatg cggtgggttc cacggatcga tggactgtcg
     1561 gacgaagggc ggcggacgcg gtggctcgcg gccgggagcc ggttcacagc actccacgac
     1621 gcggcgacct tcgaactccg tgacgaggcg gccgacctgg cgggtccgga tgtcgacggc
     1681 gcgcacgagg tcgacgcttc gggccggggc gcgaggcaaa ccgctgaccc actgctcgaa
     1741 gacacgcttc gcgaccgcct tcgcctccgc gtcatccacg aacaccgcgg gatcgaccgg
     1801 caggacggcg cgatacggcg ccgaggtggg tatctgcagc ggcggttctg cggaggcctc
     1861 gggacgacgt cgcgtcgcaa tccgcaagag ccaggcgacg aacgccacgg ccgccagcat
     1921 caccgcgtac acgcccgcgg cggcaacgac gaccatgagg aggacgagcg cgccggcgcc
     1981 gacgtcgtag cgcggcgagc gcctgtaccg acgaaccgac aaccaggcac gcgttctcat
     2041 gcatcctccc ggccatcgac caggttcggc tacggccgcg gcggagtcaa tgcagcggct
     2101 ggctgcacct gaagcccaga tgttcttgca gcgccgtgtt acagacgagg aggagcctat
     2161 cgatgaaggt cgcagcggtc gtggttggaa cggatgacga gcggttccgt cgagtggacg
     2221 ccaaccgcga gcgaatgcag ttggccgaac gcatcgccgg attcacgaag agcacggcgg
     2281 atgtcatact gctgccggcg gggttcttgg cggccaaggc cgatcgcgaa gtcaggcggc
     2341 acgccgagtc gctcgccgag gttttcggga aacaggtgtt gctcgcgggg gtcgatgaga
     2401 gcgacgaaac tcgctcagct tcgaaagatc gacgtttcgg gaagtctaag gatgcgaccg
     2461 gcaacggtga ggggcatccg ttctgggtct ttgcgtcgcg caatgggaaa ctgattggag
     2521 gcccttggca gcagcgctct gcattctccg gtgacgctgt gtcggatcct gcaccgcgct
     2581 gcgtgtcggt tggggaatcc acgatcggtg tgctgatctg cggggagctc tacaaccggg
     2641 cgatcgcgga tagcctggca gaggcaaagc cggacctggt agtcgacctc gcgcatctca
     2701 gcatgaagcg cttcaccagg agcttgcaca gggtcgcgga ggctgctgaa tgcccggtgc
     2761 tccacgttca gcacgttgcg ctcggaagcc ggaccgccgc caagtggacg gcgggcactc
     2821 aggaagcacg cggcgatcgc gccgtggact gggcttcgta caacgagccc gacttggcaa
     2881 gcgatggctt gtgggccgag gtgaagctct ggaaggtgtg accgcggatg aatgcgcggg
     2941 cagcgtacca ccgcgactgg caaggacggg ctggcgcagc gcttactaga gatccccaga
     3001 gcggtatctc ctccaatccc gcgtcggggt gctggtgcgc ttgatctggc cgcgggttgc
     3061 ctgacgctgc ggtcagcgcg cggggacgtc gtcgccgacg gattcgccga gcacgaggtc
     3121 gacctgctcg gcgtcgatgt tcgcgccgcc gacccgggcc tcgccgagct cgccgtgggc
     3181 ggagacgcgg agcgtggcgc cggacaccgt ctggatcgtg t
//
LOCUS       DASVOR010000841         4835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_300702,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000841 DASVOR010000000
VERSION     DASVOR010000841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4835
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300702"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VF516_16495"
     CDS             <1..320
                     /locus_tag="VF516_16495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109335.1"
                     /translation="AAAAAEVRSLEVRLAGLRMDVRAEIATAQTRLSAAISAMDAAAD
                     IPPILDREVQLLDKALRAGGIELAAWAQQAHRLVEVGRAYDDAVLALQRARAAWARLA
                     AQR"
     gene            complement(345..1763)
                     /locus_tag="VF516_16500"
     CDS             complement(345..1763)
                     /locus_tag="VF516_16500"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEX8109336.1"
                     /translation="MHHHARLSACLLSTGLTGLLIGLLGCGGGAPSGFSKGDHWTFPL
                     VGPLEDGVLIAPATVHGKGPYLFAIDPDASISAIDKQVADDAGLTMGRGPTLLAEDGS
                     EQSRAFAELIDLQLGTLAVDRRTVILVPTDFYNTEGRRVNGVIGRDVLADTLVFGFDR
                     DQGIAMLSTQQTFTPPPDAIAVHYNSVEADPVMVAATRGHSSGGMESSNTDDASRIGV
                     VTRDNSTSIDPTPVVRRVAAVQIGGVQVAMHLDLGGPVSQLREPLWAKVKAQPTAGKQ
                     RLVDEAATVRQVTQEAFASDVALGAARAPRITLVSYVDKRFAEGKVDGALGLDFFRSY
                     AVYANWATHTYYLKPRGDAAATATARFGRWGAALPSCAHPGCVTASLTETTGGTRLDV
                     VRDPEAAKRALEIRIGVTPAAGKSVAALVVELPAAVDKISGGVPANYEGAKATVLDTS
                     PFTRPCDSPEGCVYQFDAPAAK"
     gene            complement(2105..3667)
                     /locus_tag="VF516_16505"
     CDS             complement(2105..3667)
                     /locus_tag="VF516_16505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109337.1"
                     /translation="MHIVASSHWGRRVIVSVLCVAAAILPLAAEADPRSPWPRELLGW
                     AALGPPKTIRDLRAYADAIQPGLGTALSEVALRAALARAAGMRSLDGFDSAHWPYVLA
                     AGVDGARPIALVGRVTDARQLAASAGSACIIARSGWAVVGDERVVTRIADYALAAIAT
                     KPQPRVATAEIYLSRVLDHYRSELEIARNLWLTLATMSSGGRGAAPAGLIEALDSLAS
                     TTDRLVAMLQVTPEFSTLELRLTPKSGSWLAKLVTLQRRTSYRILERLPAGPAVMRIG
                     GHLEAGPFRDELIRLIPAHVKPRADRSGPMIIQQAASELTGDFAVAIDVSATAPVTFT
                     YLAGLTSTAAGERTLATYIAATQGGYSLQTFNGVFWAKPSPKLTIYHGVTLHSIDLVS
                     QPSTVSPAGPSPLPVDRLDGRVEYAAFDGLGMELKADDSEREAIRTIDAARGKAPHFV
                     LPPLLQASSGRGDSLVLILDIARMIEAVWGHAAAGAPPLVVTAGRADQDARITIAIPA
                     VVLRMAASLGGP"
     gene            complement(3734..>4835)
                     /locus_tag="VF516_16510"
     CDS             complement(3734..>4835)
                     /locus_tag="VF516_16510"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8109338.1"
                     /translation="AAVSSEGARLITAGRDLSRIWDMNTGAPLTRPLDEAGPSNAAAF
                     SASGARVITARANRARVWDAASGAPLGPPIVEASPVTALVFSLDGTRVVVASGDHARV
                     WDVESGTPVTAPFTHRGLVTAVAFSPDSSRVATASWDGTARVWSAQTGQPMTAPLEHG
                     AGVATVAFRPDGAVLATAGGTHARLWDATGHAVARLDHRGLVNGIAFSPDGSRLATAS
                     ADHTARLWDAATGAPVTMALEHMNAVASVAFDFDGTLLITASSTAVRIWDAATGLPVT
                     TALDHPAPVLSAVFSPDSRHIVTTTRDQVARVWDVPANRASLEAWRQRALCCPMALMR
                     GVLTAQPNPLPTCPDDSAPPSSSPSALTRSSR"
BASE COUNT          702 a         1734 c         1696 g          703 t
ORIGIN      
        1 gcgccgccgc cgccgccgag gtgcgcagcc tggaggtccg gctcgccggg ctgcgcatgg
       61 acgtgcgcgc cgagatcgcc acggcgcaga cccggctgtc cgctgcgatc tccgcgatgg
      121 atgcggcggc cgacatcccg ccgatcctcg atcgcgaggt ccagctgctc gacaaggcgc
      181 tgcgcgccgg cggcatcgag ctcgcggcgt gggcgcaaca ggcgcatcgc ctggtcgagg
      241 tcggtcgcgc ctacgacgac gccgtgctgg cgctgcagcg ggcgcgggcc gcctgggcgc
      301 gcctcgcggc gcagcgctga ccgagctcgg cggctccccg ggggctattt cgcggccggg
      361 gcgtcgaact ggtacacgca gccctcgggg ctgtcgcacg gccgggtgaa cggcgaggtg
      421 tcgagcacgg tggccttggc gccctcgtag ttcgccggca cgccgccgct gatcttgtcg
      481 acggcggccg gcagctcgac gaccagcgcc gcgaccgact tgcccgccgc cggggtcacc
      541 ccgatgcgga tctccagcgc gcgcttggcg gcctcgggat cgcgcacgac gtcgagccgg
      601 gtcccgccgg tggtctcggt caggctcgcg gtgacacagc cggggtgcgc gcagctgggc
      661 agcgccgcgc cccaccggcc gaaccgggcg gtcgcggtgg ccgcggcgtc gccgcgcggc
      721 ttgaggtagt acgtgtgggt cgcccagttc gcgtacaccg cgtacgaccg gaagaagtcg
      781 aggccgagcg cgccgtcgac cttgccctcg gcgaaccgct tgtcgacgta cgagaccagc
      841 gtgatccgcg gcgctctggc cgcgccgagc gccacgtcgc tcgcaaacgc ctcctgggtg
      901 acctggcgca ccgtcgcggc ctcgtcgacc agccgctgct tgccggcggt cggctgggcc
      961 ttgaccttgg cccacagcgg ctcgcgcagc tgactgaccg ggccgcccag gtcgaggtgc
     1021 atcgcgacct ggacgccgcc gatctgcacg gccgcgaccc ggcggacgac cggcgtcgga
     1081 tcgatgctcg tcgagttgtc gcgggtcacc acgccgatcc gcgacgcgtc gtcggtgttc
     1141 gacgactcca tgccaccgct gctgtggccg cgcgtcgccg ccaccatcac cgggtcggcc
     1201 tcgaccgagt tgtagtggac cgcgatcgcg tccggcggcg gcgtgaaggt ctgctgcgtc
     1261 gacagcatcg cgatgccctg atcccgatcg aacccgaaca ccagcgtgtc ggcgagcacg
     1321 tcgcggccga tcacgccgtt cacccggcgg ccctcggtgt tgtagaagtc cgtcgggacc
     1381 aggatcacgg tccgccggtc gaccgccagc gtcccgagct ggagatcgat gagctcggcg
     1441 aacgcgcgcg actgctcgct gccgtcctcg gccaagagcg tcggcccgcg gcccatggtc
     1501 agcccggcgt cgtcggcgac ctgcttgtcg atcgccgaga tgctggcgtc ggggtcgatc
     1561 gcgaacaggt acggcccctt gccgtggacg gtggccggcg cgatcagcac gccgtcctcg
     1621 agcggcccga ccagcgggaa cgtccagtgg tcccccttcg agaaccccga cggagctccc
     1681 ccgccacagc cgaggaggcc gatcaggagg ccggtcaagc cggtcgagag caggcatgca
     1741 gacagacgcg cgtggtggtg caccgtgggt tccttccgtg agggcggctt gcggttgggc
     1801 cgcagctgaa cccagcacgc tcccgagcgc aagcgtgagc agcatttccc gaccgcggag
     1861 cgaatccctc cacggtcggg ccagttggct cggcgcgggg cgtgttgctt ggaggttgtt
     1921 ctcgtggtgc cggggatcaa gctccgcccc agcatcgcgc ttagcacgct cggcgcgagg
     1981 cgacgttcgt caacttcacc gacctgctcc gaagccaccg cggttcctcg cggcgctgcg
     2041 gattcgacgg ccgcaacgct ctccagctgg gctaccggct cgcggacgaa gcagatgaag
     2101 gtgttcatgg tccacccaag ctcgccgcca tgcgcagcac aaccgctgga attgcgatag
     2161 tgatccgggc gtcttggtca gcgcgccccg cggtcacgac cagcggcgga gcgccggccg
     2221 ccgcgtgtcc ccagaccgcc tcgatcatcc gtgcgatgtc gaggatgagc accaagctgt
     2281 ccccccgccc gctcgatgcc tgcagtagcg gcggcaagac gaaatgcggt gccttgccac
     2341 gcgccgcatc gatcgtgcga atggcctcgc gctcgctatc gtccgccttg agctccatgc
     2401 cgaggccgtc gaacgccgcg tactccacgc ggccatcgag ccggtcgacc ggaagcggcg
     2461 acggtcccgc cggcgatacc gtcgaaggtt ggctcacgag atcgatgctg tgcagcgtga
     2521 caccgtggta aattgtcagt tttgggctcg gcttggccca gaacacgccg ttgaacgtct
     2581 gcaacgagta ccccccctgg gtcgccgcga tgtacgtcgc gagcgtgcgc tcgcccgcgg
     2641 cggtactggt gagcccagca aggtacgtga atgtaacggg cgcggtggcc gagacgtcga
     2701 tcgccacggc gaagtcacca gtcaactcgc tggccgcctg ctggatgatc atcggaccag
     2761 accggtcggc ccgcggcttg acatgcgccg ggatcaggcg gatgagctcg tcgcgaaacg
     2821 ggcccgcctc caggtgaccg ccgatccgca tgactgcggg gcccgcaggc agccgctcga
     2881 ggatcctgta gctcgtgcgc cgctgcagcg tgaccagctt cgccagccat gaaccggact
     2941 tgggggtgag gcgcagctcg agcgtggaga actccggtgt gacctggagc atcgccacca
     3001 gtcgatcggt cgtcgaggcg agcgagtcga gtgcttcgat cagccctgcg ggggctgcgc
     3061 cacgaccacc gctcgacatc gtcgcgaggg tgagccacag gttgcgcgcg atctcgagct
     3121 ccgatcgata gtgatcgagg acgcgcgaca ggtagatctc ggcggtggca acccgcggct
     3181 gcggcttcgt cgcgatcgcc gccagcgcat agtcggcgat gcgagtaacc acccgctcgt
     3241 cccccacgac cgcccagccg ctccgtgcga tgatgcacgc tgagccggcg ctcgccgcga
     3301 gctgccgggc gtcggtcacc cgtccgacca gagcgatcgg ccgagcgccg tcgactcctg
     3361 cagcgagcac gtatggccag tgcgcggaat cgaagccatc gagggaccgc atgcccgctg
     3421 cccgtgccaa cgccgcacgc agcgccacct ccgacagcgc agtccccaag cccggctgga
     3481 tcgcatcggc gtacgcccgc agatcgcgga tggttttcgg tggaccgagc gcggcccagc
     3541 cgagcaactc gcgaggccat ggcgaccgcg gatccgcctc ggcagcgaga ggcaggatcg
     3601 ctgccgcaac gcagagcact gagacgatga cgcgacgacc ccagtgagaa ctagcgacga
     3661 tgtgcattgt caaccatcct tcctcgtgtc gatgcagatg cttgcagttg ccagagcagc
     3721 gcaggctgcg cggtcagcga gacgatcggg tcagtgcact cggtgaactc gatggcggtg
     3781 cgctgtcgtc ggggcacgtc ggcaaggggt tcggctgcgc ggtgaggacg ccgcgcatga
     3841 gcgccatcgg gcaacacagc gcgcgctgcc gccacgcctc cagcgaggcg cggttggccg
     3901 gcacgtccca cacccgggcg acctgatcac gcgtcgtggt gacgatgtgc ctgctgtcgg
     3961 ggctgaacac ggcgctcagc accggtgcag ggtgatcgag cgcggtggtc accggcagac
     4021 cggtcgcggc atcccatatc cggacggccg tactgctcgc cgtgatcagc agcgtgccgt
     4081 cgaagtcgaa cgcgaccgac gccacggcat tcatgtgttc gagcgccatc gtcaccggcg
     4141 cgccggtggc cgcgtcccac agccgcgctg tgtggtctgc gctcgcagtc gcgagtcgcg
     4201 atccgtctgg actgaacgcg atcccgttga ccagtccgcg atgatcgagc cgcgccaccg
     4261 cgtggccggt ggcatcccac agccgcgcgt gggtcccgcc cgcggtcgcc agcaccgcgc
     4321 cgtcgggccg gaacgccacg gttgcaaccc cagcgccgtg ctcgagcggc gctgtcatgg
     4381 gttggccggt ctgtgcactc cacacgcgcg cggtcccatc ccagctcgcg gtggcgacgc
     4441 ggctgctgtc ggggctgaac gcaaccgcgg tcacgaggcc gcggtgcgtg aaaggcgcgg
     4501 tcactggtgt gcccgactcc acgtcccaca cccgcgcgtg gtcgccgctc gccaccacga
     4561 cacgcgtacc atcgaggctg aacaccaagg cggtcaccgg actcgcctcg acgatcggcg
     4621 ggccaagcgg cgctccgctc gcggcgtccc acacccgcgc ccggttcgcg cgcgccgtga
     4681 tcacccgcgc gccgctcgcg ctgaacgccg cggcattgct cggtccggcc tcgtcgagcg
     4741 ggcgggtcag cggtgcgccg gtgttcatat cccagatccg ggacagatcg cgacctgccg
     4801 tgatcaagcg cgcgccctcg gagctgaccg cggcc
//
LOCUS       DASVOR010000842         6263 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387955,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000842 DASVOR010000000
VERSION     DASVOR010000842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6263
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387955"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VF516_16515"
     CDS             complement(<1..349)
                     /locus_tag="VF516_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109339.1"
                     /translation="MLWYSLLAALGLLFVLSVSLPVPRRIWALAIYSVKYGLLWLTDV
                     VGLRKLYLMATGRGGKYQRLTRPMLIRMWCEDMGPTFIKFGQIVASSAGMFPDAYVKE
                     FQKVLDRVKPFPFE"
     gene            478..1329
                     /locus_tag="VF516_16520"
     CDS             478..1329
                     /locus_tag="VF516_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8109340.1"
                     /translation="MLTTVTAGPHSVRGISVGGVYTSLAVPELGLLFDAGAPLRSLAA
                     IDTILLSHGHADHVGALPALLGIRGLASKPRPPRVVMPAEIEHDLTAALAALSRLQRF
                     PLDIEPIGLRPGDELALRGDLSVRAVRTFHPVPSLGYVVVRRVAKLRPEFQGMTGPQI
                     GALRRGGTDVTEYDDRLELAYATDTLISALDHAPELYEAGTLILECTFLDERKSLAAA
                     RAGCHIHLDEVIERAERFANRHVVLMHFSQLYRPDEIPGILDARLPPELRRRVVPFIP
                     GGGHWPG"
     gene            1332..1667
                     /locus_tag="VF516_16525"
     CDS             1332..1667
                     /locus_tag="VF516_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX8109341.1"
                     /translation="MAKRPGPGPDDIDADLLDGGPPDPEATPPLTDELDLHTFRPRDC
                     ADVVAEYLRAAQDAGLPRVRIVHGKGTGALRRIVHGVLDGHPAVRGYRLAGEHGGWGA
                     TVVELLPQT"
     gene            2296..2508
                     /locus_tag="VF516_16530"
     CDS             2296..2508
                     /locus_tag="VF516_16530"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HEX8109342.1"
                     /translation="MGDEHPRRFEAPHRNALWQFDFTELRVGCERRWLLVIEDDFSRF
                     VVGHVLAEGPTSEVVVAALQDAIRQD"
     gene            complement(2505..2759)
                     /locus_tag="VF516_16535"
     CDS             complement(2505..2759)
                     /locus_tag="VF516_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109343.1"
                     /translation="MAWPIAAVSRFAHGLTNHTQRGGCEWQVIVGPADILKRWGSYFI
                     DKITVPEIDAWVPDLTTNYSPETIWHHVGLFRRIVGKARG"
     gene            complement(3103..3727)
                     /locus_tag="VF516_16540"
                     /pseudo
     CDS             complement(3103..3727)
                     /locus_tag="VF516_16540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISKra4 family transposase"
     gene            complement(3905..4783)
                     /locus_tag="VF516_16545"
     CDS             complement(3905..4783)
                     /locus_tag="VF516_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109344.1"
                     /translation="MPETRAEKKRLENAERHKQLEERRAVEVARCKSAPKEKKEEGAL
                     DVLGKKIAIVAGLFGIVSTVVSVYLAFLKIPNERNRIDAEVAKLQADVKKLNSDVTLT
                     TSQVELSKAQLLKLRNETKDARSPNFAAKAPPEVGLEEAGQYPIHWTIENKKQATNLT
                     VLAYGFRVWSATRKEVDVPDGKQPWNKLQSVAIYVSQDAVPVGGTSLFVNISRGYTIG
                     AASTIQLDGWLDRAPKPGEIVEAFMFAGELADGHCAIISLPQEKGHVGNFRPVSDDGT
                     ISNEDCLAEFSFEVVK"
     gene            5386..5628
                     /locus_tag="VF516_16550"
     CDS             5386..5628
                     /locus_tag="VF516_16550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109345.1"
                     /translation="MRTGNAHAATHHVSPSGHDDSTGQPTIVANAKGTFVAAWNAGRN
                     LVATTRLERDRASGAPVTVGSNLGSISLAIASGGPS"
     gene            complement(5798..6118)
                     /locus_tag="VF516_16555"
     CDS             complement(5798..6118)
                     /locus_tag="VF516_16555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8109346.1"
                     /translation="MFDTLEMSLTVLEQLAPVETKIRQRRKSLADEIGRAAESVALNV
                     SEARQRAGLDRADLFRRASGSASELTTALRIARARGYITAAEHAAVDAALDRVRAMLW
                     RLTH"
BASE COUNT         1024 a         2133 c         2047 g         1059 t
ORIGIN      
        1 cctcgaacgg gaacggcttg acccggtcga gcaccttctg gaactccttg acgtaggcgt
       61 cggggaacat cccggcggac gacgcgacga tctggccgaa cttgatgaac gtcgggccca
      121 tgtcctcgca ccacatccgg atcagcatcg ggcgggtcag ccgctggtac ttgccacccc
      181 ggccggtggc catcaggtac agcttgcgca gccccacgac atcggtgagc cacagcagac
      241 cgtacttcac gctgtagatc gcgagcgccc agatccggcg cggcaccggc aacgataccg
      301 acagcacgaa caggagcccc agcgcggcca gcagcgagta ccaaagcatg ctcagcgcca
      361 atcctagcac caaggtcgtg cgccccggcc cggcagccgt gccggaacca cggcgaattc
      421 cggctaacgg tgacggtgag cgccggtgaa ccaccatgcg ccccgtatac tcgcgcgatg
      481 ttgacgaccg tcaccgctgg accgcattcg gtgcgcggga tctcggtcgg cggcgtctac
      541 acctcgctcg cggtgcccga gctcggcctc ctgttcgacg ccggcgcccc gctgcgctcg
      601 ctggcggcga tcgacacgat cctgctgtcg cacggccacg ccgatcacgt cggcgcgctg
      661 cccgcgctgc tcggcatccg cggcctggcg agcaagcccc ggccaccgcg cgtcgtgatg
      721 ccggccgaga tcgagcacga cctcaccgcc gcgctcgccg cgctgtcgcg gctgcagcgg
      781 ttcccgctcg acatcgagcc gatcggcctg cgccccggcg acgagctcgc gctgcgcggc
      841 gacctcagcg tccgcgccgt gcgcacgttc cacccggtgc cgtcgctggg ctacgtggtg
      901 gtgcggcggg tcgccaagct gcgccccgag ttccagggca tgaccgggcc gcagatcggg
      961 gcgctgcggc gcggcggcac cgatgtcacc gagtacgacg accggctcga gctcgcctac
     1021 gcgaccgaca cgctgatctc ggcgctcgac cacgcccccg agctgtacga ggccggcacg
     1081 ctgatcctgg agtgtacgtt cctcgacgag cgcaagtcgc tcgccgccgc gcgcgccggc
     1141 tgccacatcc acctcgacga ggtgatcgag cgcgccgagc ggttcgccaa ccgccacgtc
     1201 gtgctgatgc acttctccca gctgtatcgc cccgacgaga tcccgggcat cctcgacgcc
     1261 cggctgccgc ccgagctgcg ccgccgggtc gtgccgttca tccccggcgg cgggcactgg
     1321 ccggggtagc cgtggcgaag cgccccggtc ccggccccga cgacatcgac gccgacctgc
     1381 tcgacggcgg cccgcccgac cccgaagcca ccccgccgct caccgacgag ctcgacctcc
     1441 acacgttccg gccgcgcgac tgcgccgacg tggtcgccga gtatctgcga gccgcgcagg
     1501 acgccggcct gccccgggtc cggatcgtcc acggcaaggg caccggcgcg ctgcgccgga
     1561 tcgtccacgg cgtgctcgac ggccaccccg cggtgcgcgg ttaccggctc gccggcgagc
     1621 acggcggctg gggcgcgacc gtggtcgagc tgttgccgca aacctaggtg tcgtagcgtc
     1681 acacgtcttg ccgctttcgg tgtcgataaa tccgcaagtc ttcccccacg caggggcgac
     1741 gtagaggcgg gcgccacagg ccggcgatgc cggtcaggag gcttcgtgat cacttctcat
     1801 tcgtctcgta aaagtacggt gtttcgaagt ccggttcgcg ccgcggcaat cgagctcgca
     1861 ttccggcgcg ggggcggagg tactcgcccg cggagcatgc gcgtgcgctg tcgctgatcc
     1921 ttgccgggat gcagcgggag taggtcgcga ctgagatcgg ctgttcgacg gagtcgctgc
     1981 gtcggtggta cgcggagggc aagaagcagg gtctggtgac cgagcctggc caagggaaag
     2041 acacgtcggt tgcggcgagg ccggccgcgg cggagccagg cacggcgacg agcgcgccgc
     2101 acgaccccgg cgcagggctc ggcgagcacg aggtcaaggc gatcctcgac tacaagaaga
     2161 agcacccgtc gatggggccg gcgcagatcc gggctcagct caatcggttc ctgggctggc
     2221 gggtgtcggt caaggcgatc gcccgcgtgc tgcgcaaggc cggggtgcgc gccggtgcat
     2281 cgcaagggcc gcccggtggg cgacgagcac ccgcggcggt tcgaggcgcc gcaccgcaac
     2341 gcgctctggc agttcgactt caccgagctt cgcgtcggct gcgagcgccg ctggctgctc
     2401 gtgatcgagg acgacttctc gcggttcgtc gtcggacacg tgctcgccga gggccctacg
     2461 agcgaggtcg tggttgcggc gctgcaggac gcgatccggc aggactagcc acgcgccttg
     2521 ccgacgatgc gccggaacaa cccgacatga tgccagatcg tctcggggct ataattcgtc
     2581 gtcaagtcgg gaacccaagc atcgatctcg ggcaccgtga tcttgtcgat gaaataggat
     2641 ccccaccgct tgaggatgtc tgccggccca acgataacct gccactcgca tccacccctc
     2701 tgcgtgtggt tggtcaggcc gtgcgcgaag cggctcaccg ctgcgatggg ccaggccatg
     2761 tacgaagcga ccccgctacc gcggcggcca ggccatgagc ctactgcagc gatcgcgaat
     2821 aatccgctag tcgccgtgac tcctgcgcat gtcaccgtgg cttcggcgca tcgactcggt
     2881 ctcgttgtga tcttccatgg aacctctcgg gtgtgatgag gacggcctcc acgagttaag
     2941 catccttcgg gctgaacgca agctggtggc cagggcatta gaaaacgggt gtggctcttt
     3001 cagatcatag ctcgacggga gaggtttcga tcacttgacg agcttcagat gggccggcgg
     3061 ggtggcagcg ggttcggcac ggtggcgtcg gcatagtgac tggtgagtgc gccggtgctc
     3121 ctgtgcgagg tggaacggca gtagtcctcg aagtcaccgc tggcgcggag cgcacgcagt
     3181 cgcaagacgg cttcgcccca gtcaacgacc agcgggctcg ggttcgaccc aggcggtcct
     3241 tcaccagata gcgacaggca ccctcgatta cgccgggcgc gatcggcagg ccatcggcga
     3301 gcgcacggtc gtagtgaaga agcttggtgt tgttgaccag gtagccgcgg cccgttcgac
     3361 cggcttgccg gcgtcacgtc caacgcgtgg ctcttgatca tcgcccgggg gctcttggcc
     3421 acatcgccgc cgccgcggcc ctggagcaga gccaggagtc gagcatcgac ccactgctcg
     3481 gcttcgtcac cgccttcggc gtgaaatgcg taggcggcgc gccacaggca ctcgatcacg
     3541 tgcaccacat cgagcacgat cgtgatcagg acaccgaagc tgtgagcggc atgccggatg
     3601 cgccggagct ggtcctcgtt gccgtcgacg agcacgaccc agcgacgacg atgctcagga
     3661 tctcgccgca gtgcttcctc gaatgcatct tcgatgacgc gctgggcacg tgctcgacgc
     3721 tcgccacacc cgcttactcg tgggatgcgg ccatcgcttc gctgtggcga tcgtcgcggc
     3781 gcatgcggag gacatcaggc ggcagacgct acccgctgac gaccgatccg gatagatcca
     3841 tcgcggtcaa cctcgcggaa tcgatcagct ttccatcgtc gcagaagatc cgcaccatta
     3901 gaaactactt gaccacctcg aacgagaact ccgcgagaca atcctcgttg gagatcgtcc
     3961 cgtcatcgga gacgggacga aaattcccga cgtgcccctt ctcttgtggc agagaaatga
     4021 tcgcacaatg accatccgca agttcgccag caaacatgaa cgcctcgact atttcgcccg
     4081 gcttcggtgc tctgtcaagc cagccgtcga gctgaatagt ggaagccgca ccgatggtat
     4141 atcccctgga tatgtttaca aacaaactgg tgccccccac gggcactgcg tcttgactga
     4201 catatattgc tactgattgc agcttgttcc acggctgctt cccatccgga acatctactt
     4261 cttttcgggt cgcgctccag accctgaagc catacgccaa cacggtcaag ttagtggctt
     4321 gcttcttgtt ctcgatcgtc cagtgtatgg ggtattgacc ggcctcttcg agacccactt
     4381 ccggaggggc cttggccgcg aagtttggag aacgagcatc cttcgtttcg tttcgcagct
     4441 taagaagttg cgctttggac agctccactt gggatgtggt tagggtaacg tcagagttta
     4501 atttcttgac gtccgcttgg agctttgcga cttcagcgtc gattctattt cgctcgttgg
     4561 gtatcttcaa gaaggcaagg tagactgaaa caacagtgga cacgatgcca aacaagccag
     4621 cgacgatcgc aatcttctta cccaggacat ctagggcacc ctcctccttt ttttctttgg
     4681 gagcgctctt gcaccgtgcc acctcaaccg cccgccgttc ctccagttgc ttgtgacgct
     4741 ccgcattctc aagacgcttc ttttctgctc ttgtttccgg cacggctcct catctcctct
     4801 tttgcgtcaa tgttagcaat gccaaagcca gcgtaccatg ggttcgcagg accgagttac
     4861 cgcggtcaca gtgtcgccaa gcgttggcca tcggcgcttt cggctttcgg gaagaggctc
     4921 aagccgttgt tgccgacctg gcgacgctgg agcgccacga ctgccagcgt acacgcgagg
     4981 tcgtgcacag agggagggtg cagctgccgc tccgtagcta ctactgggac ccagcataac
     5041 gagttccagg gccactcgtg ctgcggcggc gctggcgacc aactcggccc ggctccaatc
     5101 acgtagcggg taggaatgcc acggggacat gcctggttgt tttaggccat cgcctgcagc
     5161 cgcggatctc ggctgtggta gatggcgcgg ttcgcggcct gggctgccgc agcgcagcga
     5221 agtcacggcc agcacgttcg cccacgacag ctcggccagg gcccgcggat cgtcggtgag
     5281 cgggcagtga ccaactcgct gaactcgtgg ctgctgcgcc gcgggagctg agcgtcgacg
     5341 ggcctcgtcg cgcatgccgc aatggcttga ccgtgtccac cacccgtgcg cactggcaat
     5401 gcccacgccg ccacgcacca cgtatcgccg tccggccacg acgacagcac cggccagccg
     5461 accatcgtcg ccaacgccaa gggcaccttc gtggcggcgt ggaacgccgg ccggaatctc
     5521 gtggccacga ctcgactcga gcgcgaccgc gcgtccggcg ctccggtcac ggtcgggtcg
     5581 aacttgggca gcatcagcct ggccatcgcc tccgggggac cgtcctgatg ttgaccagtg
     5641 taacaacggg tgcccgccgt tacagccgcc gccggcgacc cgcgagcgca gatccgccgc
     5701 gagcggccgg ctgggtcgcc ctccaagccg cgcgccaggc cgatcacgag cgccgcagcg
     5761 gggatccccg ctgcgcgatg gggaatcgag gcggtgctca gtgggtaagc cgccacagca
     5821 tcgcgcgaac ccgatccagc gcggcgtcga ccgcagcgtg ttcggccgcg gtgatgtagc
     5881 cgcgcgctcg tgcgatgcgc agcgccgtgg tcagctcgct ggccgagcct gaggcgcgcc
     5941 ggaacaggtc agcgcggtcg aggccggcgc gctgccgcgc ttcgctgacg ttgagggcga
     6001 ccgactcggc ggcgcggccg atctcgtcgg cgagcgactt gcggcgctgg cggatcttgg
     6061 tctcgacggg tgcgagttgc tccagcacgg tgagcgacat ctcgagcgta tcgaacatcg
     6121 gaacctcctg gtgattcgcc cccagcaccg ggggcgtcgt cgcccgacgc gccgcggagc
     6181 gcatgtcccg tgacgagcgc gggcaccgcg acccgcggcg cgtcgatgga tccagctgtg
     6241 ggggcgccgc tcgcgtcccg tcg
//
LOCUS       DASVOR010000843         2674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_125095,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000843 DASVOR010000000
VERSION     DASVOR010000843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125095"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="VF516_16560"
     CDS             <1..236
                     /locus_tag="VF516_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109347.1"
                     /translation="ARAAALRLLSRGDGLNLSMWARALSRTADRAGLLLCGDVPAAFA
                     GAREIGELDRDLVEFAYSAAHVKLRVQLGHTRG"
     gene            277..1545
                     /locus_tag="VF516_16565"
     CDS             277..1545
                     /locus_tag="VF516_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HEX8109348.1"
                     /translation="MTDHELIYDWNSIEKVAPLSPRRRVQFLDETLRDGIQSPSVVDP
                     TIEDKLRLVELANDLGIDTMDIGLPGAGKRAVDDVTTIAEHIKASRLRIKPGCAARTH
                     QHDVQAVIDISQKVGIEIEILCFIGSSPIRQYAEDWDLARMLKLSADAIDLGRRYNLP
                     VSYVTEDTTRSRPEVLTRLFTNAVEHGASRLVVCDTVGHATPDGIRNLLKFTRYVLDG
                     AGCPQVGIDWHGHNDRGLGVVNSIFAIEFGADRIHGTALGIGERVGNAALDQILLNLK
                     LLGELPDRDLSKLLEWTQTATRATHVPIHPQYPLSGSDAFRTATGVHAAAIIKAEKKG
                     DHWLADRIYSGVPAGMFGKEQSIEVGHYSGESNVVYWLKKRGYEPTKELVAAVLGAAK
                     SGNHVLGDAEIQAVIERVHGRGTGHAGPSH"
     gene            complement(1537..2436)
                     /locus_tag="VF516_16570"
     CDS             complement(1537..2436)
                     /locus_tag="VF516_16570"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8109349.1"
                     /translation="MGLTALLVLAVTSFAAATVNGALGYGYSSISVPIALLVTVGRVL
                     NPALVIVEVAINLYALYWNRRTAGRVIRRVIPLAIGLVPGVIAGALLLGRVEPTTMKV
                     IVFSALLPLILLQASGRRWPIRRERRAAVPLGAGVGVVYGLTTISGPPLALFWNNQGF
                     AKEDFKVALAIVRSIESVCALLAYAYLGLLTSESAAILPWIVPGVLIGFPLGHVLIQR
                     IGVETFRRICMSFDAYIVAFGLSRTLDSVGVPSVAAYQVMIAASILDTALLWTFFRRR
                     QPEPPLLEPTLPPPSAAGATGSV"
BASE COUNT          450 a          954 c          932 g          338 t
ORIGIN      
        1 aggcccgggc cgccgcgctg cgcctcctgt cgcgcggcga cggcctcaac ctgtcgatgt
       61 gggcgcgcgc gctgtcccgc accgccgacc gcgccggcct gctgctgtgc ggcgacgtgc
      121 cggccgcgtt cgccggggcc cgcgagatcg gcgagctcga ccgcgatctg gtcgagttcg
      181 cgtacagcgc cgcgcacgtc aagctgcgcg tccagctcgg gcacacccgc ggctgaccgg
      241 ttatagtacc cgccaagcag gcgaagcgag gttcgcatga ccgaccacga gctgatctac
      301 gactggaact cgatcgagaa ggtcgcgccg ctgtcgccgc gccgccgcgt gcagttcctc
      361 gacgagacct tgcgcgacgg catccagtcg ccgtcggtgg tcgatccgac gatcgaggac
      421 aagctgcggc tggtcgagct cgccaacgac ctcgggatcg acacgatgga catcgggctg
      481 cccggcgccg gcaagcgcgc ggtcgacgac gtcacgacga tcgccgagca catcaaggcc
      541 agccggctgc ggatcaagcc gggctgcgcg gcgcgcaccc accagcacga cgtccaggcg
      601 gtgatcgaca tctcgcagaa ggtcgggatc gagatcgaga tcctgtgctt catcggcagc
      661 tcgccgatcc ggcagtacgc cgaggactgg gacctggcgc gcatgctcaa gctcagcgcc
      721 gacgcgatcg acctcggccg ccgctacaac ctgccggtgt cgtacgtcac cgaggacacc
      781 acgcggtcgc ggcccgaggt gctgacccgg ctgttcacca acgcggtcga gcacggcgcg
      841 tcgcggctgg tcgtgtgcga caccgtcggc cacgccacgc ccgacgggat ccgcaacctc
      901 ctgaagttca cccgctacgt gctcgacggc gccggctgcc cccaggtcgg gatcgactgg
      961 cacggccaca acgaccgggg cctgggcgtg gtcaactcga tcttcgcgat cgagttcggc
     1021 gccgatcgca tccacggcac cgcgctcggc atcggcgagc gggtcggcaa cgccgcgctc
     1081 gaccagatcc tgctcaacct caagctgctc ggcgagctgc ccgaccgcga tctgagcaag
     1141 ctcctcgagt ggacccagac cgcgacgcgc gcgacgcacg tcccgatcca cccccagtac
     1201 ccgctgtccg gaagcgacgc gttccgcacc gcgaccgggg tccacgccgc ggcgatcatc
     1261 aaggccgaga agaagggcga ccactggctc gccgaccgga tctactcggg cgtcccggcg
     1321 gggatgttcg gcaaggagca gtcgatcgag gtcggccact actccggcga gtccaacgtg
     1381 gtgtactggc tcaagaagcg cggctacgag ccgaccaagg agctggtcgc cgcggtgctc
     1441 ggcgccgcca agagcggcaa ccacgtgctc ggcgacgccg agatccaggc cgtgatcgag
     1501 cgggtccacg ggcgcggcac cggccacgcc gggccgtcac actgacccgg tagctccagc
     1561 cgccgacggc ggcggcaagg tcggctccag cagcggcggc tcgggctggc ggcggcggaa
     1621 gaacgtccac aagagcgcgg tgtcgaggat cgacgccgcg atcatcacct ggtacgcggc
     1681 gaccgacggc acgccgacgc tgtccagcgt gcgcgacagc ccgaacgcca cgatgtacgc
     1741 gtcgaagctc atgcagatcc ggcggaacgt ctcgacgccg atccgctgga tcagcacgtg
     1801 gccgagcggg aagccgatca gcacgccggg cacgatccac ggcaggatcg cggcgctctc
     1861 ggacgtgagc aggccgaggt aggcgtaggc gagcagcgcg cacaccgact cgatcgatcg
     1921 cacgatcgcg agcgcgacct tgaagtcctc cttggcgaac ccctggttgt tccagaacag
     1981 cgcgagcggc gggccggaga tcgtcgtcag gccgtagacc acgccgaccc cggcgccgag
     2041 cggcaccgcg gcgcggcgct cgcggcggat cggccagcgc cggcccgacg cctggagcag
     2101 gatcagcggc agcagcgcgc tgaacacgat caccttcatg gtggtgggct cgacgcggcc
     2161 gagcagcagc gcgccggcga tcacgccggg caccaggccg atcgccagcg ggatcacgcg
     2221 gcggatcacg cggcccgcgg tgcgccggtt ccagtacagc gcgtacaggt tgatcgcgac
     2281 ctcgacgatc accagcgcgg ggttgagcac ccggccgacc gtgaccagca gcgcgatcgg
     2341 caccgagatc gacgagtagc cgtagccgag cgcgccgttg accgtggcgg cggcgaagct
     2401 cgtgacggcg aggacgagga gagcggtgag acccatcgag acccctgggc gagtgccagc
     2461 agccgaaccg gcgttataac ggatgcgtac cccatatccg ttatcttagc tacccacgat
     2521 atttcgtgga aaacgcgtga cctcggcgcg cgcaggacct atcttcggcc catggcgatc
     2581 cccggcgatg gcgagcggct cgacgtggcc ggtgcgtccg gagttcagcc gcgcggcgag
     2641 ccggtgcggt cgtccggcga ggcgttcccc acgc
//
LOCUS       DASVOR010000844         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_66656,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000844 DASVOR010000000
VERSION     DASVOR010000844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66656"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..676)
                     /locus_tag="VF516_16575"
     CDS             complement(101..676)
                     /locus_tag="VF516_16575"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8109350.1"
                     /translation="MDRVEHLTESEGIEMSDELPEMIPPVDNAPAAPVLRREALLPNE
                     RSRGLGTVAIVCTMAGVASGLALATTLMAMQVADSMSSRGCPYREAQIVPQAGVRTDH
                     GFLGVTYYPRDGYADIEQVLPGTPAAAVDLRPGDHVLAVNHITLGSPSSDLTERIFEC
                     HPGDTATLLVERHGERLTVRPTLVAWPTISY"
     gene            869..>1693
                     /locus_tag="VF516_16580"
     CDS             869..>1693
                     /locus_tag="VF516_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109351.1"
                     /translation="MALVVVVTAVYGLLRARFSGAELGDNIASILNKRMRGRIEIGSI
                     EWSSASLQKVLTGGWVPVTIRDVRVWDDCALSAAITGDEGDELRTGDPNEDCTPDDRP
                     DLDPTSRRKPRKLLLRTDLVTADIDIHAVMFGHHDFVFRNLWVHGGEALLEQTREPYP
                     LHAYDRTIVSLVTAFYPRQLPGFRAGITAGDPPPIFDLRDIHIAGLNLTLHMSPYTSA
                     RSPSIGYGFTARLEGVDVDASTDPANSSYLYMDATDPLVAKFYVRLAAAARRGTVRI"
BASE COUNT          267 a          601 c          573 g          252 t
ORIGIN      
        1 ccaccgacta ctgcagcctg tgacggagct gccccgcgag ctcggccccg gcgcagccgg
       61 gacgccgatc gacggcgacg atcgacccgc ggcgtaggga tcagtacgag atggtcggcc
      121 acgccaccag cgtcggccgc accgtcaggc gctcgccatg gcgctcgacg agcagggtcg
      181 ccgtatcccc cgggtggcac tcgaagatcc gctcggtgag atcgctgctg ggcgagccca
      241 gcgtgatgtg gttcaccgcc agcacatggt cgccgggacg gaggtccacg gcggctgcgg
      301 gcgtccccgg cagcacctgc tcgatatccg cataaccgtc gcgcggatag tacgtaactc
      361 ccaggaatcc gtgatccgtg cggacgccgg cctggggcac gatctgcgcc tcgcgatacg
      421 gacacccccg ggaggacatg ctgtcggcga cctgcatcgc catgagcgtc gtggcaagcg
      481 cgagtcccga ggccacgccg gccatcgtgc agacaatggc gaccgtgccc agcccgcggc
      541 tgcgctcgtt cggcagcaac gcctcgcgcc ggaggacggg ggcggcgggc gcgttgtcaa
      601 ccggggggat catctcgggc agctcgtcgg acatctcgat tccctcgctt tcggtcaagt
      661 gttcgacccg gtccatgcgg gtcgcttcgg ccgtgcctat gtcgagtata gggggggccc
      721 cccaacatga ccacgacgaa atgcgccggg cggacgcaat cgtgaagccg cgcacgcccg
      781 agtgcgtcga atggccatgg tagattccgg agctccggtg aacacgcccg tccctcgacg
      841 caggcgccgg tggctcacag ccatcggctt ggcgctggtg gtggtggtga ccgccgtgta
      901 cggcctgctg cgcgcgaggt tctccggcgc cgagctcggc gacaacatcg cgtcgatcct
      961 caacaagcgg atgcgcggcc ggatcgagat cggatcgatc gagtggtcga gcgcgtcgct
     1021 gcagaaggtt ctcaccggag gctgggtccc ggtgacgatc cgcgacgttc gcgtgtggga
     1081 cgattgcgca ctgagcgctg cgatcacggg cgacgagggc gacgagctgc gcaccggcga
     1141 ccccaacgag gactgtacgc cggatgaccg accggatctc gacccgacgt cccggcgcaa
     1201 gccgcgcaag ctgctgctgc gcaccgacct ggtgaccgcc gacatcgaca tccacgccgt
     1261 gatgttcggc caccacgact tcgtgttccg caacctgtgg gtccatggcg gagaggcgct
     1321 gctcgagcag acccgcgagc cgtacccgct gcacgcctac gaccgcacga tcgtcagcct
     1381 ggtcaccgcg ttctacccgc gccagctgcc ggggttccgc gccgggatca ccgccggcga
     1441 cccgccgccg atcttcgacc tgcgcgacat ccacatcgcc gggctcaacc tcacgctgca
     1501 catgtcgccg tacacgtcag cgcggtcgcc gtcgatcggc tacggcttca ccgcgcggct
     1561 cgagggtgtc gacgtcgacg ccagcaccga tccggcgaac agctcgtatc tctacatgga
     1621 cgcgaccgac cccctggtcg ccaagttcta cgtccggctc gcggccgcgg cgcggcgtgg
     1681 cacggtgcga atc
//
LOCUS       DASVOR010000845         3842 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_387973,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000845 DASVOR010000000
VERSION     DASVOR010000845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387973"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VF516_16585"
     CDS             complement(<1..1007)
                     /locus_tag="VF516_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109352.1"
                     /translation="MLAGKTIGVLAVIGIAGVTGAVGVLVSSSATPRPLAHAPVAPDQ
                     PARPRPRPQLTTTSDVRATALHRAHLQAAERVDCASCHDMRAREFRAPPVGTCLGCHT
                     GHEPAVHGSAPEASCFTCHDFLRPDGASAWARRCETCHDRAQGSYPAIAIHQKQGCGT
                     CHPVHPRRGVRPADCLDCHDTRVTGHPANGSGRAVCRTCHLPHRPAREALARCQACHA
                     AGARDVPVEVRIPPTALFRGHDRCLQCHTGHTFTRASVKPCRDCHAGKHVLAADRVPA
                     HAACTSCHDPHAAQSTPVARCAICHAAIAASSKHPRDPGGRDCVGCHPPHQELGPSRA
                     AL"
     gene            988..2265
                     /locus_tag="VF516_16590"
                     /pseudo
     CDS             988..2265
                     /locus_tag="VF516_16590"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
     gene            complement(2590..>3842)
                     /locus_tag="VF516_16595"
     CDS             complement(2590..>3842)
                     /locus_tag="VF516_16595"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8109353.1"
                     /translation="APASLDLAADRAVVPVAVPVPPSGGSVGFTFAEDRRGWVARIPE
                     HLQLPAVAYGAGKIYVSGGFESVSFYGLDAETGRIDWATTNLEDNGPTAAVFEDDRVL
                     FNTESCTLFALDARTGRRLWFRKLGDPTLAQVTTADGLVYASHPGESGQELTAYRVKD
                     GEPVWTRSIGAELLAAPVISGDAIYATNLHGVVFRFARRTGKLAWARGLDATTAPWIT
                     DNELFVTRQVGRREQQIVVAADTGKLLRAHDQSSGHHAGDVPGDARALGNDSWKQVWA
                     FEGSRPVVDRGVRYVAMGGEIRASDAATGELLWQRRYAGAPVDRRSVGSVALAGSEVV
                     IATRDGKLFGLDVDTGYTLWSYAIGHRVIAEPVIARGWVYTSTEDGYVVALQVGDRTL
                     DGWHMFGGNPRHDGPVASPPPRGV"
BASE COUNT          500 a         1365 c         1442 g          535 t
ORIGIN      
        1 agcgccgcgc gcgacgggcc gagctcctgg tgcggcggat gacagccgac gcagtcgcgg
       61 ccgcccggat cgcgcgggtg cttcgagctc gcggcgatcg cggcatggca gatcgcgcac
      121 cgcgcgaccg gcgtcgactg cgccgcgtgc ggatcgtggc agctcgtgca cgccgcgtgc
      181 gcaggcaccc ggtcggcggc gagcacgtgc ttgcccgcgt ggcagtcgcg gcacggcttg
      241 accgacgcgc gggtgaacgt gtggccggtg tggcactgca ggcatcgatc gtggccgcgg
      301 aacagcgccg tcggcggaat ccgcacctcg acgggcacgt cgcgcgcacc ggccgcgtgg
      361 cacgcctggc agcgcgcgag cgcctcccgg gcggggcgat gcggcaggtg gcaggtccgg
      421 cacaccgccc gccccgatcc gttcgcgggg tggccggtga cccgggtatc gtgacagtcg
      481 aggcagtccg cgggccggac gccgcgccgc gggtggaccg gatggcaggt gccgcagccc
      541 tgcttctggt ggatcgcgat cgccgggtac gagccctggg cacggtcatg gcaggtctcg
      601 caccggcgcg cccacgccga cgcgccgtcc ggacgcagga agtcgtggca ggtgaagcag
      661 ctcgcctccg gggcgctgcc gtgcaccgcg ggttcatggc cggtgtggca gcccaggcac
      721 gtgccgaccg gcggcgcgcg gaactcgcgg gcgcgcatgt cgtggcagct cgcgcagtcg
      781 acccgctcgg cggcctggag gtgcgcgcgg tgcagtgccg tcgcgcggac gtcgctcgtc
      841 gtcgtcagct gggggcgcgg gcgcggccgg gccggctgat caggtgcgac cggagcgtgc
      901 gcgagaggcc gcggggtagc gctcgacgag accagcacgc cgaccgcgcc ggtcacgccg
      961 gcgatcccga tcaccgcgag cacgcctatg gtcttccccg cgagcacgga tctgcacctc
     1021 caggaacggt atccccagtc gccagcccct ttggagaggc ggccgtgcat cacgcagacc
     1081 agcgatcggc gcaccgattc gcgatcatcg tgcagttcgc gcgtccggac cggcgaagct
     1141 tcgcaagtcc tgcgcagata cggaatccga tccagcgagt gcgcagaacg cagcctcggc
     1201 cggcccgcgc aatcgccgcg cgacgacgca aacggctcgt gcgacaactt gccagctctg
     1261 ctcggcgacg agcagcgtaa catcatccat gtgaaggtca tcgcgtccac cggcgagacc
     1321 atcgcgagca tcgcccgcgc ggtggtcatt gcatcggtcg cggcgctgtg cgtggtcgtc
     1381 tatgccgccg gcacgctgcg cggcctcgcg atccgcgacc ggagcgcgcg cgatcggcac
     1441 cgcgatcgcc ggcgcggacg cttgttgctc tggtcgttcg cgcggctggg cgcgacgttc
     1501 gtcaagatcg gccaggtcgc aggctcgcgg ccggacctgt tctccccggg cgtgatcgcc
     1561 gagctgcgcc cgctccagga tcacgtcccg ccgtttccct atcgccgggt gcgtacgatc
     1621 ctcgcgcgcg cgctcggcgc gccgatcgaa gcgcggttcc gcgagttcga tccggtgccg
     1681 gtcgcggcgg gcagcgtcgc gcaggtccac cgggctgtgc tgagcggctg cgacgaggtc
     1741 gcggtgaagg tgctgaggcc cgggatccgc gagcaggtgc gccgcgacgc gcggctgctg
     1801 ctctggctcg cccacgtcgt gcatgcgctg tcgccgcgcg cccgcgccgc cgacgtgatc
     1861 ggccacgtgc ggagcctggt caccgggatc gccgcgcaga ccgagctgcg ctacgaggct
     1921 cgcaactacc ggcggttccg ccgcgacttc gccggctcgc gcacggtggc attcccgctg
     1981 gtctacccgc agcactcgac gcgcgacgtg ctcgtgatgg agttcgtgca cggcgtgacc
     2041 ctcgatcggc tgcgccccga gcaggtcccg caggtgtcgc gcgcgctgcg cgaggcgttc
     2101 ttcgcgatgt gcttcgaaca cggacacgtc cacgccgacc tgcactccgg caacgtgctc
     2161 gtgcgcgacg acggggtcgt cgtgctggtc gacgtcggcc tggtcaagta cctctcggcc
     2221 ggcgtgatcg atcagctgat cgacttcggg cgctgcaccg cgatcggtga cgcgcgcgat
     2281 ctggtcgcgc acctgcgcac gcactaccgc tgccacgcga ccaccgactg gggagcggtg
     2341 gcggccgatg cggcggcgtt gatcgcggag ctgcgccggc gatcgctggc ggagatcgag
     2401 gtcagctccg tcgtcgggca cctgttcgcg ctcgcgcgca agcatcggat ccgtccggtg
     2461 ccggagctga ccctcgtgct gctcggcatg gtcacgatcg agggcctcgc gaagcgcctc
     2521 gatccgacgg tgaacatgat gtccgaggtc gcggcgttcc tcggcccgcg catcgcacaa
     2581 gggcgccggc tacacgccgc ggggcggcgg cgaggcgacc gggccgtcgt ggcgcgggtt
     2641 gccgccaaac atgtgccagc cgtcgagggt gcggtcgccg acctgcagcg cgacgacgta
     2701 gccgtcctcg gtgctggtgt agacccagcc gcgcgcgatc accggctcgg cgatcacgcg
     2761 gtggccgatc gcgtacgacc atagggtgta gccggtgtcg acgtcgagcc cgaacagctt
     2821 gccgtcgcgg gtggcgatca cgacctcgga gccggcgagc gcgaccgagc cgaccgagcg
     2881 gcgatcgacg ggggcgccgg cgtagcggcg ctgccagagc agctcgccgg tggcggcgtc
     2941 gctcgcgcgg atctcgccgc ccatcgcgac gtagcgcacg ccgcggtcga cgacggggcg
     3001 cgagccctcg aaggcccaca cctgcttcca gctgtcgttg ccgagcgcgc gggcgtcgcc
     3061 cggcacatcg ccggcatggt ggccggatga ctggtcgtgg gcgcggagca gcttgccggt
     3121 gtcggcggcg acgacgatct gctgctcccg gcgcccgacc tggcgggtga cgaacagctc
     3181 gttgtcggtg atccacggcg cggtggtcgc gtcgagcccg cgggcccatg ccagcttgcc
     3241 ggtccggcgg gcgaaccgga acacgacgcc gtgcaggttc gtggcgtaga tcgcgtcgcc
     3301 cgagatcacc ggcgcggcga gcagctcggc gccgatcgac cgggtccaca ccggctcgcc
     3361 gtccttgacc cggtacgcgg tgagctcctg gccggactct ccggggtgcg atgcgtagac
     3421 caggccgtcg gcggtggtga cctgcgccag cgtcggatcg ccgagcttgc ggaaccacaa
     3481 ccgccggccg gtcctggcgt cgagcgcgaa cagggtgcag ctctcggtgt tgaacaggac
     3541 gcgatcgtcc tcgaacaccg cggcggtcgg gccgttgtcc tccaggttgg tcgtcgccca
     3601 gtcgatccgg ccggtctcgg catcgagccc gtaaaagctg accgactcga agccgccgga
     3661 gacgtagatc ttgccggcgc cgtaggcgac cgcgggcagc tgcaggtgct cggggatccg
     3721 cgcgacccag ccgcggcgat cctcggcgaa cgtgaacccg accgagccgc ccgacggcgg
     3781 gaccgggacc gcgaccggca ccacggcgcg atcggccgcg agatcgaggc tcgcgggtgc
     3841 gg
//
LOCUS       DASVOR010000846         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_125120,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000846 DASVOR010000000
VERSION     DASVOR010000846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125120"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1449
                     /locus_tag="VF516_16600"
     CDS             151..1449
                     /locus_tag="VF516_16600"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEX8109354.1"
                     /translation="MAEGKTDKPPLQPAQVCGAKYLDRVFALLAKLRDDGCARDKAGN
                     RTLYYDRYASLVLLSMFSPAMDSLRAIQEASALAKVQKLFGTDRRFSLGSLSEAARVF
                     DPQLLEAVVAELGRELLPLADCHDPRLAPVRQDLRRTVTLVDGTLLKALPRLVASMWT
                     TSRSGNPMHGWRLHCQFDLSLGVPLEAGLTDYRNSGESDERAVLEANLRPGRCYVMDR
                     GYFQYGLFDAISLVGSDYVCRVKRSIAYEVVLERTVDAAGRAAGVTRDVVVRAGSDER
                     VERRANHAVRLVYVEAEVCPRAKRGSTKPAGPAEKQVLILATNMLDVPADVVALLYRY
                     RWTVELFFRFFKRVMGCRHLLSDDPDGVRIQCYCAVIACMLLALWTGRKPDKSTWRMV
                     GWFLSGMAQESEVLAHLSRRPDNRGVKLAAKEALWKKLGL"
BASE COUNT          232 a          539 c          613 g          269 t
ORIGIN      
        1 ccggcgttca ggttggcgga agcggtggtt ggtgttttgg gtgccattat ggcaggtggc
       61 tttgttccca atactgttcg gcacggcgtt cgcgcgagca gcaagatctc gacttgcgcc
      121 cgttccgcgg gtgtggcatg atcgcgcgtg atggccgagg gcaagaccga caagccgccg
      181 ctccaacccg cccaagtctg cggggccaag tacctcgacc gggtcttcgc gctcctggcc
      241 aagctgcgcg acgacgggtg cgcccgcgat aaggccggca accgcacgct ctattacgac
      301 cgctacgcgt cgctcgtgct gctctcgatg ttcagcccgg cgatggactc gctgcgggcc
      361 atccaggagg cctcggcgct ggcgaaggtg cagaagctgt tcggcaccga ccgccgcttc
      421 tcgctcggct cgctgtccga ggcggcgcgg gtgttcgacc cacagctcct ggaggcggtg
      481 gtcgccgagc tgggcaggga actgctgccg ctggccgact gccacgaccc gcggctcgcc
      541 ccggtgcggc aggacctgcg gcggacggtc acgctggtcg acggcacgct gctcaaggcc
      601 ctgccgcggc tggtcgcgtc gatgtggacg accagccgca gcggcaaccc gatgcacggc
      661 tggcggctgc actgccagtt cgacctctcg ctgggcgtgc cgctggaggc ggggctgacg
      721 gattatcgca acagcggcga atccgacgag cgcgccgtgc tcgaggcgaa cctgcggccc
      781 ggccgctgtt acgtgatgga ccgcgggtac ttccagtacg gcctgttcga cgcgatctcg
      841 ctggtcggca gcgactacgt ctgccgggtg aagcggtcga tcgcctacga ggtggtgctg
      901 gagcgaacgg tcgacgccgc gggccgggcg gcgggggtga cgcgggacgt ggtcgtgcgg
      961 gccggctcgg acgagcgcgt cgagcggcgg gcgaaccacg ccgtgcggct ggtctacgtc
     1021 gaggcggagg tttgtccgcg ggccaagcgg ggctcgacca agcccgccgg cccggcggag
     1081 aagcaggtgc tgatcctggc gaccaacatg ctggacgtgc cggcggacgt ggtcgcgctg
     1141 ctctaccggt accgctggac ggtcgagctg ttcttccgct tcttcaagcg ggtgatgggg
     1201 tgccggcacc tgctcagcga cgaccccgac ggcgtgcgga tccagtgcta ctgcgcggtg
     1261 atcgcgtgca tgctgctggc gttgtggacc ggccgcaagc ccgataagtc cacgtggcga
     1321 atggtcggtt ggttcctgag cgggatggcc caggagtcgg aggtgctggc gcacctgagc
     1381 cggcggccgg acaaccgggg cgtgaagctg gcggccaagg aggcgctctg gaaaaaactg
     1441 ggcctgtagc gccggggcga tcgcgtcgcg cgcgacccgc ccgggttcac gctgcgcgca
     1501 tttaatccga aaccgcggcg cccccgctcc ccccgcgtca aagccccgtc ccgccccgcc
     1561 ccaacccgcc gcgccaccgc cgcagcaagg gttgggccgg acagtattgc tttgttttgc
     1621 gcggatgggt gagtggaaga ttgtggaaca ttc
//
LOCUS       DASVOR010000847         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_37557,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000847 DASVOR010000000
VERSION     DASVOR010000847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37557"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VF516_16605"
     CDS             <1..611
                     /locus_tag="VF516_16605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109355.1"
                     /translation="FTTRQIRGGVPPGVVALGRILFELQGCNTCHGGGKWTISTKDFV
                     SPPAAGDIFTERTGMFSEQPVPNQFLARFLVDIGSFNLGVAGAVPPNPIGGDIGAAEF
                     ATAQVIAGMFVLQPGALGFDYNNDGKGNGFNVPSLLGIGNLQPYYHNGACETLACVVS
                     DVNHRTAMGRLPDILEHPVLQALVVKFVESIDADTRPFASTR"
     gene            complement(841..>1811)
                     /gene="lipA"
                     /locus_tag="VF516_16610"
     CDS             complement(841..>1811)
                     /gene="lipA"
                     /locus_tag="VF516_16610"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEX8109356.1"
                     /translation="MPDIESRVHLPVVGQPAAAPSAAPGDEPAPRRRHPPWIRATLPT
                     KPAYFELRAMVKELQLHTVCESASCPNIGECWTRRALTIMILGDICTRSCRFCDVTTG
                     RPLPPDPDEPRRVAEMLGRLSLEHTVITCVDRDDLADFGAAHWAETIRRVKAACPAMT
                     LEVLAGDFKGKTELVDVVLDADPDVFAHNLETVPRLSRAVRVQASYPRSYRILEHARK
                     RDAVTKTGLMLGLGETRDEVEQVLRDLAGLGVDIVTLGQYLAPSKQHLAIERYVPPAE
                     FAELEQHARDVGITHVVAGPLVRSSYHADGQAQLVRELRAAKRPAT"
BASE COUNT          251 a          626 c          669 g          265 t
ORIGIN      
        1 cgttcaccac gcggcagatc cggggcggtg tgccgcccgg tgtcgtggcc ctggggcgga
       61 tcttgttcga gctgcagggc tgcaacacgt gccacggcgg cggcaagtgg acgatcagca
      121 ccaaggactt tgtctcgccg ccggcggcgg gcgacatctt caccgagcgc acgggcatgt
      181 tctcggagca gccggtgccg aaccagttcc tggcgcggtt cctggtcgac atcggatcgt
      241 tcaacctcgg cgtcgcgggg gctgtcccgc ccaacccgat cggcggggac atcggtgcgg
      301 cggaattcgc caccgctcag gtcattgccg gcatgttcgt cctgcagccg ggcgcgctcg
      361 gcttcgacta caacaacgac ggcaagggca acggcttcaa cgtgccgtcg ctgctcggca
      421 tcggtaacct gcagccgtac taccacaacg gcgcgtgcga gaccctggcg tgcgtcgtga
      481 gcgacgtgaa ccatcgcacg gcgatggggc ggctgcccga catcctcgaa caccccgtgt
      541 tgcaggcgct ggtggtgaag ttcgtcgagt cgatcgacgc cgacaccagg ccgttcgcca
      601 gcacccggtg atggttcgcc cgcgtgcgtg acggccgctg aacgccagcc gtcgggccgg
      661 cgctccgcga cccgaccagg gggctctgct caagcgccac tcagagccgg cgggagccga
      721 gggcgagacg accgacgatc acgccgcgct gggggctgcc gacgacgagc tgcggactgc
      781 atccgggatc gacgacggca cgagcctgcc ggtccaggac agcgagatcc agagccgccg
      841 ctatgtcgcc gggcgcttgg cggcgcgcag ctcgcggacc agctgggcct ggccgtcggc
      901 gtggtagctc gaccgcacca gcggcccggc gaccacgtgg gtgatgccca cgtcgcgggc
      961 gtgctgctcg agctcggcga actcggcggg cgggacgtag cgctcgatcg ccaggtgctg
     1021 cttgctcggc gcgaggtact ggccgagcgt gacgatgtcg acgccgagcc cggcgaggtc
     1081 gcgcagcacc tgctcgacct cgtcgcgggt ctcgccgagc ccgagcatca ggccggtctt
     1141 ggtgaccgcg tcgcgcttgc gcgcgtgctc gaggatccgg tagctgcgcg ggtagctggc
     1201 ctggacgcgg accgcgcgcg acagccgcgg cacggtctcc aggttgtggg cgaacacgtc
     1261 gggatcggcg tcgagcacga cgtcgaccag ctcggtcttg cccttgaagt cgccggcgag
     1321 cacctcgagc gtcatcgccg ggcacgccgc cttgacccgg cggatcgtct cggcccagtg
     1381 cgcggcgccg aagtcggcca ggtcgtcgcg gtcgacgcag gtgatcaccg tgtgctcgag
     1441 cgaaagccgg ccgagcatct cggcgacccg gcgcggctcg tcgggatccg gcggcagcgg
     1501 ccggccggtg gtgacgtcgc agaaccggca cgaccgggtg cagatgtcgc cgaggatcat
     1561 gatggtgagc gcgcggcggg tccagcactc gccgatgttc gggcagctcg cgctctcgca
     1621 caccgtgtgc agctgcagct ccttgaccat cgcgcgcagc tcgaagtacg ccggcttggt
     1681 cggcagcgtc gcgcggatcc acggcggatg ccgccgccgc ggcgcgggct cgtcccccgg
     1741 tgcggcgctc ggtgcggccg cgggctgtcc gacgaccggc aggtgcacgc gcgattcgat
     1801 gtccggcatc g
//
LOCUS       DASVOR010000848         5360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_505006,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000848 DASVOR010000000
VERSION     DASVOR010000848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505006"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1719
                     /locus_tag="VF516_16615"
     CDS             <1..1719
                     /locus_tag="VF516_16615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX8109357.1"
                     /translation="GFEVIVSGAQKGVGSSRETAAQCEVFSGSRLAIASSFAPIHARN
                     NINIGQLMGDHDMLRRLERGESIPLAEFTRGHDAITARIIESGGLLPFCARLSRGEIA
                     VPPTGTAPRPMNLTEKILARKLLPGQGSHVKPGDAVLVKVDTGYSHEFTTAQVDKFLA
                     DEYGAGYRLADPHRFAVFEDHLIYATGVAAMARFADKIEELRERQRRFAAATGVRNYS
                     AVDGVSPGICHQVAREQLIDPGDFVQATDSHTCMGGASGALAWGVGATEYAALIYWGY
                     TPIAVPEAIRFELAGRLPAGTSAKDVMLHILATYARREDTLNRVMEFGGDGLFALSPD
                     ERATLANMATECSARGAVMEVDDIMLRWIAERRGGDPARTVDALRAKVVAPDPGAEYA
                     GGVHRIDLAQIRPMVATPGDPDRGIASDPKNGALIPEIGRVTIHIAYGGSCTAGKRDD
                     LDMYAQVMAEAERAGKKVADGVRFYIQFGSQEVEAYARDRGYTDLFARTGVEVIKPGC
                     GACIGCGPGVSDRADQVTVSAINRNYKGRSGPGRLYLASPLTVAASAVTGEIVEYHAG
                     MFSRRP"
     gene            1879..2499
                     /locus_tag="VF516_16620"
     CDS             1879..2499
                     /locus_tag="VF516_16620"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8109358.1"
                     /translation="MKLLYQTHSPHARKVLVMAHEVGIADRLEVIHQETSPMVRNDAV
                     FALNPLGKVPVLVLSDVDCLFDSMVICEYLDGLHDGPRMFPPHGAARWHALRLQALAQ
                     GMADAGIQLRWEIERRPEAFRWPKLGDGYRDKLVTSYDFIEAEISLDGPVDIGQISLA
                     CTLSWLAFRDLPWFGDRHPRLAVWFEAFGHRPSMARTPLSGQTHDR"
     gene            2766..3122
                     /locus_tag="VF516_16625"
     CDS             2766..3122
                     /locus_tag="VF516_16625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109359.1"
                     /translation="MWRTAVVLIAITLAACSSDPRPAIDAGPPPIVDAPAGCPALPDA
                     AHFDFFGDVCSAAPFPANTVCHTDQNGDHGWCIGPDSGGAGTCRPMAYDASRCPVCPS
                     GTQRFAPAGGSYCAPN"
     gene            complement(3129..4307)
                     /locus_tag="VF516_16630"
     CDS             complement(3129..4307)
                     /locus_tag="VF516_16630"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX8109360.1"
                     /translation="MPVDALVDRSARRDSGGIGGFVIYVADARNDTIRKITAAGVVTT
                     FAGVAGDPGSADGTGAGARLSDPDGVAVDGAGTVYVADTFNSTIRKITAAGAVTTLAG
                     AAGFTGTDDGTGAAARFNLPEAVAVDGAGNVYVADTQNFTIRKITADGVVTTLAGVAG
                     VAGSADGTGAEAQFGNPTGLAVDGAGNVYVADRDNATIRKVTPAGVAVDGAGNVFVAD
                     LGNFVIRKVTPAGVVTTFAGTAGMRGSVDGTGPAARFEVVTDLAIDDAGNLYVADFGA
                     NVIRKITSAGVVTTLAGTADTSGSADGTGAAARFNRPTGVAVDPAGNVYVTDTQNHTL
                     RKITPAGVVTTVAGTAGVAGIVLGATPGLAFPEYLAIVGDSIVCTDNRAVLRLRHGAR
                     "
     gene            complement(4406..>5360)
                     /locus_tag="VF516_16635"
     CDS             complement(4406..>5360)
                     /locus_tag="VF516_16635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109361.1"
                     /translation="GTYDLKTTPLPWPPANPDGSLVAPPTAPCGIQRPGINTTPAVAA
                     DGTIYVVSRAHGNPRYAYVIAVGPDLTPRWATSLRDILHDNCGVTVASDATATERTTH
                     CRTGTPTGVERVTGLAPAAQVDDQSSSSPVALPDGGVLYGAFTGYNSARGHLFKLDRS
                     GAVAATFDFGWDSTPAVVVGLDDYRIVVKDNHYGTDTSGVDLGPFYITELDAQLRVLW
                     QFQSTNTKSCARQPDGSIACTSDHPHGFEWCINAPAVDRDGTVYANSEDGTTYAITAN
                     GQLRDAIFLDRALGAAYTPVALDRSGRVFALNAGRLAVLGR"
BASE COUNT          744 a         1924 c         1911 g          781 t
ORIGIN      
        1 ggcttcgagg tcatcgtgtc cggcgcccag aagggcgtcg gcagtagccg cgagaccgcc
       61 gcgcagtgcg aggtgttctc cggcagccgg ctcgccatcg cgtcgtcgtt cgcgccgatc
      121 cacgcccgca acaacatcaa catcggccag ctgatgggcg accacgacat gctgcgccgg
      181 ctcgagcgcg gcgagtcgat cccgctggcc gagttcacgc gcggccacga cgcgatcacc
      241 gcgcggatca tcgagagcgg cggcctgctc ccgttctgcg cccggctgtc gcgcggcgag
      301 atcgccgtgc cgccgaccgg caccgcgccg cggccgatga acctgaccga gaagatcctc
      361 gcccgcaagc tgctccccgg ccagggcagc cacgtcaagc ccggcgacgc cgtgctggtc
      421 aaggtcgaca ccggctactc gcacgagttc accaccgccc aggtcgacaa gttcctcgcc
      481 gacgagtacg gcgccggcta ccggctcgcc gacccgcacc ggttcgcggt gttcgaggac
      541 cacctgatct acgccaccgg cgtcgccgcg atggccaggt tcgccgacaa gatcgaggag
      601 ctgcgcgagc gccagcgccg gttcgccgcc gccaccggcg tgcgcaacta ctccgcggtc
      661 gacggcgtgt cgcccggcat ctgccatcag gtcgcccgcg agcagctgat cgaccccggc
      721 gacttcgtcc aggccaccga ctcgcacacc tgcatgggcg gcgcctcggg cgcgctcgcg
      781 tggggcgtcg gcgccaccga gtacgcggcg ctgatctact ggggctacac gccgatcgcc
      841 gtccccgagg cgatccggtt cgagctcgcc ggccggttgc ccgccggcac ctcggccaag
      901 gacgtcatgc tccacatcct cgccacctac gccaggcgcg aggacaccct caaccgcgtc
      961 atggagttcg gcggcgacgg cctgttcgca ctgtcgcccg acgagcgcgc caccctcgcc
     1021 aacatggcca ccgagtgctc ggcgcgcggc gcggtcatgg aggtcgacga catcatgctg
     1081 cgctggatcg ccgagcggcg cggcggcgac ccggcccgca ccgtcgacgc gctgcgcgcc
     1141 aaggtcgtcg cccccgaccc cggcgccgag tacgccggcg gtgtccaccg catcgacctc
     1201 gcgcagatcc gcccgatggt cgccaccccg ggcgatccgg accgcggcat cgccagcgac
     1261 cccaagaacg gcgccctgat ccccgagatc ggcagggtca cgatccacat cgcctacggc
     1321 ggctcgtgca ccgccggcaa gcgcgacgac ctcgacatgt acgcccaggt catggccgag
     1381 gccgagcgcg ccggcaagaa ggtcgccgac ggcgtccggt tctacatcca gttcggctcc
     1441 caggaggtcg aggcctacgc cagagaccgc ggctacaccg acctgttcgc gcgcaccggc
     1501 gtcgaggtca tcaagcccgg ctgcggcgcc tgcatcggct gcggccccgg cgtcagcgac
     1561 cgcgccgatc aggtcacggt ctcggcgatc aaccgcaact acaagggccg ctccggcccc
     1621 ggccggctct acctcgcctc gccgctcacc gtcgccgcct cggcggtcac cggcgagatc
     1681 gtcgagtacc acgccggcat gttctcgcgg cgcccgtagc cctctactcg ccggagatga
     1741 tgacgtcctt gttgctgtac gcgtacgcgc ccggtggccc tcgtcgcgcg tccggccagg
     1801 cgcatcgcaa cgaggggagc ggcggcgacc tgcggccctg gggcacgcgg gcggcgctat
     1861 gcttgtcccg gagccaagat gaaactcctg taccagacgc actcgccgca cgcccgcaag
     1921 gtgctcgtga tggctcacga ggtcggcatc gccgatcgcc tcgaggtgat ccaccaggag
     1981 accagcccga tggtccgcaa cgacgccgtg ttcgcgctca acccgctggg caaggtcccg
     2041 gtgctggtcc tctcggatgt cgactgcctg ttcgactcga tggtgatctg cgagtacctc
     2101 gacggcttgc acgacggccc gcggatgttt ccgccgcacg gcgccgcgcg ctggcacgcc
     2161 ctgcgcctgc aggcgctggc gcaaggcatg gccgacgccg ggatccagct gcgctgggag
     2221 atcgagcgcc ggcccgaagc gttccggtgg cccaagctcg gcgatggcta ccgagacaag
     2281 ctggtcacga gttacgactt catcgaggcc gagatctcgc tcgacggtcc ggtcgacatc
     2341 ggccagatct cgctggcctg cacgctgagc tggctcgcgt tccgcgacct gccgtggttc
     2401 ggcgaccgcc atccgcgact cgcggtgtgg ttcgaggcat tcggccatcg gccgtcgatg
     2461 gccaggaccc cgctgtccgg ccagacccac gatcggtgag ccggcgtgcg gactaccgct
     2521 cctccaccgc gatcccttcg accgcctgct catcgcgcag gcccggatcg agggaatgac
     2581 gatcctgacc cgcgaccaca tcatcaccca gtacgatgtg gccacgttcc cggcttgaga
     2641 atcgcgagga cgtgcccgat gccggctact gggccctgct agcggcgcgc gcgaggctga
     2701 ctcagatttg gagtggaatg cggcaggttg caggcggggg ctgggcgacc tacgctcgcg
     2761 gctgcatgtg gagaaccgcg gtggtgctga tcgcgatcac gctcgcggcg tgctcgagcg
     2821 accctcggcc ggccatcgac gccgggccgc cgcccatcgt cgacgcaccc gcgggctgcc
     2881 cggcgctgcc ggacgcggcg cacttcgact tcttcggcga cgtgtgctcc gcggcgccgt
     2941 tccccgcgaa caccgtatgc cacaccgatc agaacggcga tcatggttgg tgcatcggcc
     3001 ccgacagcgg cggtgcgggg acgtgtcggc cgatggcgta cgacgcaagc cgctgcccgg
     3061 tctgcccctc tggcacccag cgtttcgccc cggccggcgg gtcctactgt gcgccgaact
     3121 gacggccgct acctggcgcc gtggcggagc cgcagcacgg cgcggttgtc ggtgcacacg
     3181 atggagtcgc cgacgatcgc gaggtactcc gggaacgcga gcccaggcgt cgcgccgagg
     3241 acgatgccag cgacgcccgc ggtcccggcc acggtcgtca cgacgccggc gggggtgatc
     3301 ttgcggaggg tgtggttctg cgtgtcggtg acgtagacgt tgccggcggg gtcgaccgcc
     3361 acgccggtcg gtcggttgaa gcgcgccgcg gcgccggtgc catccgcgct gcccgaggtg
     3421 tccgcggtgc cggcgagcgt ggtcacgacg cccgcggagg tgatcttgcg gatcacattc
     3481 gcgccgaagt cggcgacgta gaggttgccg gcgtcgtcga tcgccaggtc ggtcacgacc
     3541 tcgaaccgcg cagccgggcc ggtgccatcg acgctgccgc gcatgccggc agtgccggcg
     3601 aacgtcgtca ccacccccgc cggcgtgacc ttgcggatga cgaagttgcc gaggtcggcg
     3661 acgaacacgt tgccggcgcc gtcgaccgcc acgcccgcgg gggtgacctt gcggatcgtg
     3721 gcgttgtccc ggtcggcgac gtagacattg ccggcgccat ccaccgcgag accggtcggg
     3781 ttgccgaact gggcctcggc gcccgtgccg tcggcgctgc cggcaacgcc cgcgacgccg
     3841 gccagcgtcg tcacgacccc gtcggcggtg atcttgcgga tcgtgaagtt ctgggtgtcg
     3901 gcgacgtaga cgttgcccgc gccgtcgact gccacggcct cgggcaggtt gaaccgagcg
     3961 gccgcgccgg tgccgtcgtc ggtgccggtg aacccggcgg cgccggcgag cgtggtgacg
     4021 gcgccggcgg cggtgatctt gcggatcgtg gagttgaagg tgtcggcgac gtagacggtg
     4081 cccgcgccgt cgaccgccac gccgtcgggg tcggacagcc gtgccccggc gcccgttccg
     4141 tccgcgctgc ccggatcccc ggcgacgccg gcgaacgtcg tgaccacccc ggccgcggtg
     4201 atcttgcgaa tcgtgtcgtt gcgggcatcg gcgacataaa taacgaatcc tcctatgccg
     4261 ccactgtctc gtcgggcgga gcgatcgacc agggcatcaa ctggcaaaca cgatccaccc
     4321 aaaggtgatg ttgtgatctg gatatcgacc gcgaccgatc gacgtgactg cagccacgcg
     4381 tctcggcaac cgcgcgcgcc gccggctacc ggccgagcac ggcgagccgg ccggcgttga
     4441 gcgcgaacac ccggccggag cgatcgagcg cgaccggggt gtaggcggcg ccgagcgcgc
     4501 ggtcgaggaa gatcgcgtcg cgcagctggc cgttggccgt gatcgcgtag gtggtgccgt
     4561 cctcgctgtt ggcgtagacc gtgccgtcgc ggtcgaccgc gggggcgttg atgcaccact
     4621 cgaagccgtg cgggtgatcg ctggtgcagg cgatcgatcc gtcgggctgg cgcgcgcagc
     4681 tcttcgtgtt ggtgctctgg aactgccaga gcacgcggag ctgggcgtcg agctcggtga
     4741 tgtagaacgg gccgaggtcg acgccgctgg tgtcggtgcc gtagtggttg tccttgacga
     4801 cgatccggta gtcgtccagg ccgacgacca ccgcgggcgt gctgtcccag ccgaagtcga
     4861 acgtggcggc gaccgcgccg ctgcgatcga gcttgaacag gtggccgcgc gcgctgttgt
     4921 agccggtgaa cgcgccgtac agcacgccgc cgtcgggcag cgcgaccggc gacgacgacg
     4981 actggtcgtc gacctgcgcc gccggagcca ggccggtgac gcgctcgacg ccggtcgggg
     5041 tgccggtccg gcagtgggtg gtccgctcgg tggccgtggc gtcggacgcg accgtgacgc
     5101 cgcagttgtc atgcaggatg tcgcgcagcg aggtcgccca tcgcggcgtg aggtcggggc
     5161 cgaccgcgat gacgtaggcg tagcgcggat tgccgtgcgc gcgggacacc acgtagatcg
     5221 tgccgtcggc ggcgaccgcg ggagtcgtgt tgatgccggg gcgctggatg ccgcacggcg
     5281 cggtcggcgg cgcgaccagg ctgccgtccg ggttcgcggg cggccacggc agcggcgtgg
     5341 tcttcaggtc gtaggtgccg
//
LOCUS       DASVOR010000849         2900 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_476149,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000849 DASVOR010000000
VERSION     DASVOR010000849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476149"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1336
                     /locus_tag="VF516_16640"
     CDS             <1..1336
                     /locus_tag="VF516_16640"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEX8109362.1"
                     /translation="PAPSSATIELPSGRTIADDAAQDTPSVVNVFSERRVDRGDRAPG
                     LADPFFRYFFGVPRGVQRAPQRERSLGSGVIVSPDGVILTNSHVVENAETIKVALKDG
                     RELDARLVGTDPQSDIAVLRVDQTQLPAIAIADSSKSRIGDLVLAIGNPFGIGQTVTM
                     GIISAVGRANMGITDYEDFIQTDAAINPGNSGGALVDMDGKLVGINTAIASQTGGYQG
                     IGFAIPSNMAMQIETAILRDGKVTRGWLGIAVQDVTSDLAKAMDLTPHHGVLVSDVAH
                     GGPAARAGIQRGDVITAIDGTALDDAGQLRTMVALAGKNKRVAVQLERHGKPMTVQAT
                     LGEAPTTRQAQAQAQVEDGVLSGLAVQPLDRALRDRLHVPDNIVGVVVTGVDRRGGPA
                     AALLREGDVILEVNRQPVPTVEAFRDAARAAGDRALLLVDRNGVAIYVTLSR"
     gene            complement(1341..1901)
                     /locus_tag="VF516_16645"
     CDS             complement(1341..1901)
                     /locus_tag="VF516_16645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109363.1"
                     /translation="MYARSLAVHRLGILAFALAIAGCPPSPNIPRPPGTDGGARWAFA
                     IAEPVIHGIAPDAELREILGAVVNLDGRIPSNGGDWSFVAWSPTRSTIQVTVSFHGTT
                     STSQRTDAPPGPGIQVPLPANWADSIAVFAATSGKRDPGATIANLVVLNVASYTEAPG
                     KATWGINFNAGQNQLITATGSYIGPE"
     gene            complement(1997..2497)
                     /locus_tag="VF516_16650"
     CDS             complement(1997..2497)
                     /locus_tag="VF516_16650"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8109364.1"
                     /translation="MTDQPHGSSAPAPRFHYAKAAEGGYKAMLGLEAFLHSCGLEAGL
                     IHLLKLRVSQINGCAFCIDMHWKDLRALGETEQRLYSLGAWHESPFYSDRERAALAWA
                     EAVTELADGHVPDEVYRRVRGQFDEQGVANLTFVVATINAWNRLAIASRTPPGSYQPR
                     KPAQHA"
     gene            2506..>2900
                     /locus_tag="VF516_16655"
     CDS             2506..>2900
                     /locus_tag="VF516_16655"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8109365.1"
                     /translation="MTASSGRVQRGAADQIHRRVHAAHWPIPPPEAVPSIADLRGARL
                     VYQRQMEDRRAAPRQPAHVSAALETAQGRSTIAITRDISTRGLLLLTRAALQVGELVK
                     LTVALGDGPGTLSGKVVRVEDLDGHELWR"
BASE COUNT          417 a         1064 c         1019 g          400 t
ORIGIN      
        1 cccggcgccg tcgtcggcga ccatcgagct gccgtcgggg cgaaccatcg ccgacgacgc
       61 cgcccaggac accccgagcg tggtcaacgt gttctccgag cggcgggtcg accgcggcga
      121 ccgcgcgccg ggtctcgccg atccgttctt ccggtacttc ttcggcgtgc cccgcggtgt
      181 gcagcgcgcc ccgcagcgcg agcgcagcct gggctccggc gtcatcgtat cgccggacgg
      241 cgtcatcctg accaacagcc acgtggtcga gaacgccgag acgatcaagg tcgcgctcaa
      301 ggacggccgc gagctcgatg ccaggctggt cggcaccgat ccgcagagcg acatcgcggt
      361 gctgcgggtc gaccagacgc agctccccgc gatcgcgatc gcggactcgt cgaagagccg
      421 gatcggcgat ctcgtgctcg cgatcggcaa cccgttcggg atcgggcaga ccgtgacgat
      481 ggggatcatc agcgcggtcg gccgggccaa catggggatc accgactacg aggacttcat
      541 ccagaccgac gccgcgatca acccgggcaa ctcgggcggc gcgctggtcg acatggacgg
      601 caagctggtc ggcatcaaca cggcgatcgc gtcgcagacc ggcggctacc agggcatcgg
      661 gttcgcgatc ccgagcaaca tggcgatgca gatcgagacc gcgatcctgc gcgacggcaa
      721 ggtcacgcgc ggctggctcg gcatcgcggt ccaggacgtg acctcggatc tcgccaaggc
      781 gatggacctg acgccgcacc acggcgtgct ggtctccgat gtggcgcacg gcggccccgc
      841 cgcccgggcc ggcatccagc gcggcgacgt gatcaccgcg atcgacggca cggcgctcga
      901 cgacgccggc cagctccgca cgatggtcgc gctcgccggc aagaacaagc gcgtggcggt
      961 gcagctcgag cgtcacggca agccgatgac ggtccaggcg accctgggcg aggcgccgac
     1021 cacccgccag gcccaggcgc aggcccaggt cgaggacggc gtgctgtccg gcctggcggt
     1081 ccagccgctc gatcgcgcgc tgcgcgaccg gctgcacgtg ccggacaaca tcgttggcgt
     1141 cgtcgtgacc ggcgtcgatc gccgcggcgg tccggcggcg gcgctgctgc gcgagggcga
     1201 cgtgatcctc gaggtcaacc gccagccggt ccccaccgtc gaggcgttcc gcgacgcggc
     1261 ccgcgcggcc ggcgatcgcg ccctgctgct cgtcgaccgc aacggcgtgg cgatctacgt
     1321 cacgctgtcg cgctgaccgg ctactccggc ccgatgtagc tgccggtggc cgtgatcagc
     1381 tggttctggc cggcgttgaa gttgatgccc cacgtcgcct tgccgggcgc ctcggtgtac
     1441 gacgcgacgt tgagcacgac caggttcgcg atcgtcgcgc ccggatcgcg cttgccgctg
     1501 gtcgccgcga acaccgcgat gctgtccgcc cagttcgccg gcagcgggac ctggatcccg
     1561 gggcccggcg gcgcgtcggt gcgctgcgag gtcgaggtcg tgccatggaa gctcaccgtc
     1621 acctggatcg tcgatctcgt cgggctccac gcgacgaagc tccagtcccc gccgttcgac
     1681 gggatccggc catcgaggtt gaccaccgcg ccgaggatct cgcgcagctc ggcgtcgggg
     1741 gcaatcccgt ggatcaccgg ctcggcgatc gcgaacgccc accgggcgcc cccgtcggtg
     1801 cccggcggac gcgggatgtt cgggctcggc ggacagccgg cgatcgccag ggcgaacgcg
     1861 aggatcccga gccggtggac cgcgagcgag cgtgcgtaca tcatcccctc ccagacccgg
     1921 cgcaatgccg aggatctgca acctatcacg cccgcgatcc gcggaacagc tgccgcgcgc
     1981 ggccgagttt gccagctcag gcgtgctggg ccggcttgcg gggctggtag ctgcccggcg
     2041 gcgtgcgcga cgcgatcgcg agccggttcc acgcgttgat cgtcgccacg acgaacgtca
     2101 ggttcgcgac gccctgctcg tcgaactggc cgcggacccg ccggtacacc tcgtcgggca
     2161 catggccgtc ggccagctcg gtcaccgcct cggcccaggc cagcgcggcg cgctcgcggt
     2221 cgctgtagaa cgggctctcg tgccaggcgc cgagcgagta cagccgctgc tcggtctcgc
     2281 cgagcgcgcg caggtccttc cagtgcatgt cgatgcagaa cgcgcagccg ttgatctgcg
     2341 acacccgcag cttgagcagg tggatcagcc ccgcctccag cccgcacgag tgcaggaacg
     2401 cctccagccc gagcatcgcc ttgtagccgc cctcggccgc cttggcatag tggaaccgcg
     2461 gcgccggggc gctcgaaccg tgtggttggt cggtcattgc cagatgtgac cgcgagctcc
     2521 ggccgcgtgc aacgtggtgc ggcggatcag atccaccgcc gggtccacgc cgcgcactgg
     2581 ccgatcccgc cgcccgaggc cgtcccgtcg atcgccgatc tgcggggcgc ccggctggtg
     2641 taccaacgtc agatggaaga tcgacgagca gccccccgac agccagccca tgtctcggcc
     2701 gcgctcgaga ccgcgcaggg ccgatcgacc atcgcgatca cccgcgacat cagcacccgc
     2761 ggcctgctcc tcctgacccg cgcggcgctc caggtcggcg agctcgtcaa gctgaccgtg
     2821 gcgctcggcg atggccccgg cacgctgtcc ggcaaggtcg tgcgcgtcga ggacctcgat
     2881 ggccacgagc tgtggcgcta
//
LOCUS       DASVOR010000850         6505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_534502,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000850 DASVOR010000000
VERSION     DASVOR010000850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6505
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534502"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..519)
                     /locus_tag="VF516_16660"
     CDS             complement(1..519)
                     /locus_tag="VF516_16660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(241..243),aa:Sec)
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX8109366.1"
                     /translation="MTITLLDPTGERGSVERPGAPRPPALDGQVVALLDISKPRGEVF
                     LDQLATRLATRGATVRRYAKPTFTKPAPPDLRRRIAEECDAVIEALADUGSCTSCSVH
                     DITDLEGAGLPGVFVASAEFVAAAAAQARALGFDAARVFVPHPIQDRTDDEMRALADA
                     AVDEVVARLLAR"
     gene            complement(516..1964)
                     /locus_tag="VF516_16665"
     CDS             complement(516..1964)
                     /locus_tag="VF516_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX8109367.1"
                     /translation="MVEPPRLPEGLVAVVKRDCPTCGLVAPVLGELAARGRSLTVYTQ
                     DDPEFPPAVAPRIDDRELTVSWHHRIETVPTLLRIENGVEVARTEGWSRSAWEALAGV
                     SPLGAELPPHRPGCGSLSVDPVRADQLRVRFEGGRLAARRIDLGEIEDELEAAYDRGW
                     TDGLPVVPPTPARVLRMLDGTRRAPDEVVAVVPPDLVPVTVEKVAINAVMAGCKPEYL
                     PVVLAAVQAACTDRFNMHGLLCTTYFSGPVIVVNGPIAAQLGMNSGVNCLGQGNRANA
                     TIGRALQLVVRNVGGGLPGGIDRATFGAPGKYTFCFAEDEAGSPWEPLSVEQGFAPGV
                     STVSLFPGHGPSAIVDQISRTPESLARSLAAKLRATAHPKLVMGMGALLIVSPEHARV
                     FAAAGWTKARLRAALDELLLIPGEELVRGAGGIEEGMPAALAAGRRLPKFRPGDLAIV
                     HAGGSAGMFSAIIEGWVSGERGSQLTTQEVIP"
     gene            2159..2737
                     /locus_tag="VF516_16670"
     CDS             2159..2737
                     /locus_tag="VF516_16670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109368.1"
                     /translation="MSTCGRLFQVDKVVAYFVAADKHVVIVADVFQNPTADHVKICPN
                     PIAIPPAREFLVIGTTEPGIHPLRVVKRRISTTFVSDATPRSVIVYTQGIDAPVRQEV
                     SVGSVAPPPLAPGDAEPPTASRPHAPVEVTGFSEEFSLEQAMRNALTQAVAKLGGPPR
                     NPDVAIQIDIQDISASTGGNIRPGLTVRATGR"
     gene            2761..3255
                     /locus_tag="VF516_16675"
     CDS             2761..3255
                     /locus_tag="VF516_16675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8109369.1"
                     /translation="MRTGFVPTAFSVIVDAGAPVPPASEDATAARVRDAWRDASDAQF
                     ARQIKELFGHMSVKSDRLAPVVDEIVRSDRRAIGDVVYDLSVTAVRDELPRIRAPVLI
                     ILADGALQDDFRRQAHAVRDHEVVVVPDTGHFVMIDDPPAFFAVIDDFLIKHPRGAHR
                     LPES"
     gene            complement(3252..3686)
                     /locus_tag="VF516_16680"
     CDS             complement(3252..3686)
                     /locus_tag="VF516_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109370.1"
                     /translation="MRYLLLLSLAACSPTRVVVKWWSTGAGTNERIEIPSSGNGNYTR
                     TDNGRVEKDERVILTSDQVHELDELFLGQGACQLKHDPEYTPRADEGQTTLELAFPDQ
                     TCKVVLYNTEWDRGHAQPIAETMRSMRPLQRTKPTTRKVNPL"
     gene            3916..4260
                     /locus_tag="VF516_16685"
     CDS             3916..4260
                     /locus_tag="VF516_16685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109371.1"
                     /translation="MRTRPTTSFEPLGAGDLDRASGGYSQALFSAMQQAVSMGLTINS
                     TTTGGHAPNSMHYKGRAFDSIGTPAQMQSFYNWAAANTHPHELIHKNQFLKDGHPHAP
                     IGGHNGHVHFSI"
     gene            4297..4506
                     /locus_tag="VF516_16690"
     CDS             4297..4506
                     /locus_tag="VF516_16690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109372.1"
                     /translation="MTTLSSLLSGTELAAVCGGAQNPQAQAVCNRQQFDWMAAHMVPD
                     NALRPGVQRHVVAHDAQVCGFPMPR"
     gene            4545..5597
                     /locus_tag="VF516_16695"
     CDS             4545..5597
                     /locus_tag="VF516_16695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8109373.1"
                     /translation="MSAWSSVLGLSVLLLACAQAQPPPALSAPAGAPPPSSSPSAGDP
                     RFDAELSGYPYPFAVQFLELASQQETVRMAYMDVAPAQPNGHTVILLHGKNFPASTWE
                     PTIRLLTARGYRVIAPDQIGFGKSSKPARYQYSFHQLAGNTRAVLDRAGVTKVSVVGH
                     SMGGMLATRFALMFPDRVEKLVLVNPIGLEDWKTVVPYRSIDQWYEQEKKATPESVRD
                     YQRNAYYGGTWKPEYERLIETSIGVLHHPDYPKIAWASALIYDMIFTQPVVYEFPNLK
                     VPTLLIIGQRDRTALGKAGVPKEIAATLGDYPALGKRTAAAIPGAQLVEIPGVGHLPQ
                     VEAFEAYSQALAKFLP"
     gene            complement(5614..>6505)
                     /locus_tag="VF516_16700"
     CDS             complement(5614..>6505)
                     /locus_tag="VF516_16700"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109374.1"
                     /translation="LEAAQDDATRLAIADMERAGLDILTDGEIRRESYFNRFATALSG
                     IDIDQPGLVESRSGKQVPMPRVVGKVQRLRPVEQRDAEFLRRHTRRPIKITLPGPFTM
                     TQLAKDEHYGDERALAMDYADAVHQEACDLVAAGADFIQLDEPYLQAHPDKARRYGVE
                     AIDRALAGLAAPTIVHMCFGYAYVVKDKPSGYSFLPELDRCRAAQVSIEAAQPRLDLA
                     ILGALPSKTMMVGVLDLGDPAIETPDTVAARIDQALQHIAPERLVIAPDCGMKYLARD
                     VAFGKLVAMVEGTRRVRARL"
BASE COUNT          942 a         2335 c         2268 g          960 t
ORIGIN      
        1 ctaccgcgcg agcaggcgcg ccacgacctc gtcgaccgcg gcgtcggcca gcgcgcgcat
       61 ctcgtcgtcg gtgcggtcct ggatcgggtg gggcacgaac acacgcgctg cgtcgaagcc
      121 gagcgcacgg gcctgcgccg ccgcggcggc gacgaactcg gccgaggcca cgaacacgcc
      181 gggcagaccg gcgccctcga ggtcggtgat gtcgtgcaca ctgcacgacg tgcaactccc
      241 tcaatcggcg agcgcctcga tgaccgcatc gcactcctcg gcgatccgcc gccgcaggtc
      301 ggggggcgcc ggcttggtga acgtgggctt ggcgtagcgc cggacggtcg cgccgcgcgt
      361 ggcgagccgc gtcgcgagct ggtcgaggaa cacctcgccg cgcggcttgg agatgtcgag
      421 caaggcgacg acctggccgt cgagcgccgg gggccgaggg gcgcccggac gttcgacgga
      481 gccgcgctcc ccggtcgggt cgagcagggt gatggtcacg ggatcacctc ctgggtcgtg
      541 agctggctgc cgcgttctcc cgacacccag ccctcgatga tggcagaaaa catgcccgcg
      601 ctgccaccgg catgaacgat cgcgaggtcg ccggggcgga acttgggcag ccgccggcct
      661 gccgcgagcg cggccggcat gccctcctcg atcccgccgg ccccgcgcac cagctcctca
      721 ccggggatga gcaagagctc atcgagcgcg gcgcgcagcc gggccttggt ccagccggcc
      781 gcggcgaaca cccgcgcgtg ctccggtgac acgatgagca gtgcgcccat gcccatcacc
      841 agcttggggt gggcggtggc ccgcagcttg gcggcaaggc tccgggccag cgactcgggc
      901 gtccgtgaga tctgatcgac gatcgcggac ggaccgtgtc cgggaaacag ggacaccgtc
      961 gatactcccg gagcgaaccc ctgctcgacc gacagcggct cccacggcga gccggcctca
     1021 tcctccgcga agcagaaggt gtacttgccc ggagcgccga acgtcgcccg gtcgatgccg
     1081 ccgggtagcc cgcccccgac gttgcggacc accagctgca gggcccgccc gatcgtcgcg
     1141 ttggcccggt tcccctggcc gaggcaattg acgccgctgt tcatgccgag ctgcgccgcg
     1201 atcggaccat tgaccacgat gaccgggccg gaaaaatacg ttgtgcacag aagtccgtgc
     1261 atgttgaacc ggtcggtgca cgccgcctgg accgcggcga ggaccaccgg caggtactcc
     1321 ggcttgcagc cggccatcac cgcgttgatc gcgaccttct cgacggtcac cggcaccaga
     1381 tcgggcggga ccacggcgac gacctcgtcg ggagcgcgcc gcgtgccgtc gagcatgcgc
     1441 agcacccggg ccggggtcgg cggcacgacc ggcaggccgt cggtccagcc gcgatcgtag
     1501 gccgcctcca gctcgtcctc gatctccccg agatcgatcc gtcgcgccgc gagccggcca
     1561 ccctcgaagc gcacccgcag ctggtcggcg cgcaccgggt cgaccgacag cgagccgcag
     1621 cccggccggt gcgggggcag ctcggcgccg agcgggctga ccccggccag cgcctcccac
     1681 gcgctgcgcg accagccctc ggtccgcgcg acctcgacgc cgttctcgat cctgagcagc
     1741 gtcggcacgg tctcgatccg gtggtgccac gacacggtca gctcgcggtc gtcgatgcgc
     1801 ggcgccacgg ccggcgggaa ctcgggatcg tcctgcgtgt acaccgtgag cgaccggccg
     1861 cgcgccgcga gctcgccgag caccggcgcg accagtccgc aggtcggaca gtcgcgcttc
     1921 accacggcga ccaggccttc gggcagccga ggcggctcga ccatggccgc gactctagtc
     1981 cgaaagccgc gcaccggaca atcgacggcg gcgcaatgga ccatcgcgat cgtccatctg
     2041 taccggtcgg ccgccgggtg cgtcgcgcgg cgtgccgcgc gcagtttgca gtcttttgcc
     2101 ctaccgccga gcacgaacgg cgctaggctc cgcatcaggg ggcaaaagga gggtgaggat
     2161 gagtacgtgt ggaaggttgt ttcaggtcga caaggtggtt gcgtacttcg tcgcggcgga
     2221 caagcacgtc gtgatcgtgg ccgacgtgtt ccagaacccg acggcggatc atgtgaagat
     2281 ctgtcccaat ccgatcgcga tcccgccggc gcgggagttc ctcgtgatcg gtaccacgga
     2341 gcccgggatc catccgctgc gggtggtcaa gcgccggatc tcgacgacgt tcgtctcgga
     2401 cgccacgccc cggagcgtga tcgtgtacac gcaggggatc gacgctccgg tgcgccagga
     2461 agtctcggtc ggcagcgtgg caccgccccc gctggcgccc ggcgatgcgg agccaccgac
     2521 cgccagccgg ccgcacgcgc cggtcgaggt caccgggttc tcggaggagt tctcgctgga
     2581 gcaggcgatg cgaaatgccc tgacgcaggc cgtcgcgaag ctcggcggtc cgccgcgcaa
     2641 tcccgatgtc gcgatccaga tcgacatcca ggacatctcg gccagcaccg gggggaacat
     2701 tcgccctggc ctgacggtcc gggcgaccgg ccgatagccg gcgaccgcgg cgcgcccgcg
     2761 ttgcgcacgg gcttcgtgcc caccgcgttc tccgtcatcg tcgacgccgg cgcaccggtc
     2821 cccccggcgt ccgaagatgc gaccgccgcc cgggtccgcg atgcctggcg cgatgcgagc
     2881 gatgcgcagt tcgcgcgcca gatcaaggag ctgttcggcc acatgtcggt caagtccgat
     2941 cggctcgcgc ccgtggtcga cgagatcgtc cggtcggatc gccgcgcgat cggcgacgtg
     3001 gtctacgatc tgtcggtcac cgcggtgcgc gacgagctgc cccggatccg cgcgccggtc
     3061 ctgatcatcc tcgccgacgg cgcgctgcag gacgacttcc gccgccaggc ccacgcggtg
     3121 cgcgaccacg aggtcgtcgt cgtccccgac accggccact tcgtgatgat cgatgatccg
     3181 ccggcgttct ttgcggtgat cgacgacttc ctgatcaagc acccgcgcgg tgcgcaccgc
     3241 ctgcccgaaa gctagagcgg gttcaccttc cgcgtcgtgg gcttcgtccg ctgcagcggt
     3301 cgcatcgatc gcatcgtctc ggcgatcggc tgggcgtggc cgcggtccca ctcggtgttg
     3361 tacagcacga ccttgcaggt ctgatcgggg aacgccagct cgagcgtggt ctggccctcg
     3421 tcggcccgcg gcgtgtactc ggggtcgtgc ttgagctgac acgcgccctg gccgaggaac
     3481 agctcatcga gctcgtggac ctggtcgctc gtgaggatca cccgctcgtc cttctcgacc
     3541 ctgccgttgt cggtccgggt gtagttgccg ttgccgctgc tcgggatctc gatgcgctcg
     3601 ttggtgccgg cgcccgtgct ccaccacttc acgacgaccc gcgtcgggct acaggcggcg
     3661 agcgagagca ggaggaggta gcgcatcggg cgagtgtaac gcatcgcatc gcgcgtttgc
     3721 gccggccgcg gtggcaaccc gcgtggcctg gcggcaagct cggtcgccac cggtggcgct
     3781 cgatccgcgt gaccgcacgc accgccggcg ccgatctcgt ctaccgcggt cggcgatcgc
     3841 ggcgcgtcat gtcgaccgcg ggtttcggat cgcgccgggc gcgcgatggc atcgccgctg
     3901 cagtgtcgtc gcgccatgcg aacacgaccc acgacaagct tcgagccgct cggcgccggc
     3961 gatctcgacc gcgcgagcgg cggctactcc caggccctgt tctcggcgat gcagcaggcg
     4021 gtgagcatgg gcctgaccat caactcgacg acgaccggcg gccacgcgcc gaactcgatg
     4081 cactacaagg ggcgcgcgtt cgactcgatc ggcacgccgg cccagatgca gagcttctac
     4141 aactgggcgg cggcgaacac gcacccgcac gagctgatcc acaagaacca gttcctgaag
     4201 gacggccatc cccacgcgcc gatcggtggc cacaacggcc acgtccactt ctcgatctga
     4261 tcccgcgatc tgatctctcg acccgaggag ccgaccatga cgaccctgtc ttccctgctt
     4321 tccggcaccg agctcgccgc ggtctgcggc ggcgcacaga acccccaggc ccaggcggtc
     4381 tgcaaccggc agcagttcga ctggatggcc gcgcacatgg tgccggacaa cgcgctccgg
     4441 cccggcgtgc agcgccacgt cgtcgcgcat gacgcccagg tctgcggctt tccgatgccg
     4501 cgctagtcgc gacgccggcc gtggtgcgcg gtagggtacg cgtcatgtcg gcctggtcct
     4561 ccgtcctcgg tctctcggtg ctcttgctcg catgtgcgca ggcccagccg ccgccggcgc
     4621 tgagcgcgcc cgccggagcg ccgccgccct ccagctcgcc gtccgccggc gatccgcggt
     4681 tcgacgccga gctgagcggc tatccgtatc cgttcgcggt ccagttcctc gagctggcct
     4741 cgcagcagga gacggtgcgg atggcgtaca tggacgtcgc gccggcgcag cccaacggcc
     4801 acacggtcat cctgctccac ggcaagaact tcccggcgtc gacctgggag ccgacgatcc
     4861 ggctcttgac cgcgcgcggc tatcgcgtga tcgcgcccga ccagatcggc tttggcaagt
     4921 cgagcaagcc ggcgcgctac cagtacagct ttcaccagct cgccggcaac accagggccg
     4981 tgctcgaccg cgcgggggtg acgaaggtca gcgtcgtcgg gcactcgatg ggcggcatgc
     5041 tcgccacccg gttcgccctg atgttccccg accgggtcga gaagctggtg ctggtcaatc
     5101 cgatcgggct cgaggactgg aagaccgtgg tgccgtatcg ctcgatcgat cagtggtacg
     5161 agcaggagaa gaaggccacg cccgagtcgg tgcgcgacta ccagcgcaac gcctactacg
     5221 gcgggacgtg gaagccggag tacgagcggc tgatcgagac ctcgatcggc gtcttgcacc
     5281 accccgacta ccccaagatc gcgtgggcct cggcgctgat ctacgacatg atcttcaccc
     5341 agccggtggt ctacgagttc ccgaacctca aggtgccgac gctcctgatc atcggccagc
     5401 gcgaccggac cgcgctgggc aaggccgggg tgcccaagga gatcgccgcg acgctgggcg
     5461 actatcccgc gctcggcaag cgcaccgccg cggcgatccc cggggcgcag ctcgtcgaga
     5521 tccccggcgt cggccacctg ccgcaggtcg aggcgttcga ggcctattcg caggcgctcg
     5581 cgaagttcct gccgtgatcg cgctgggatg agatcacagc cgcgcgcgca cccggcgcgt
     5641 gccctcgacc atcgcgacga gcttgccgaa cgcgacgtcg cgcgccaggt acttcatccc
     5701 gcaatccggc gcgatcacca ggcgctcggg cgcgatgtgc tgcagcgcct gatcgatgcg
     5761 cgccgcgacg gtgtccggcg tctcgatcgc cggatcgccg aggtcgagca cgccgaccat
     5821 catcgtcttg ctcggcagcg cgccgaggat cgcgaggtcg agccgcggct gcgccgcctc
     5881 gatcgagacc tgcgcggcgc ggcagcggtc gagctcgggc aggaacgagt acccgctcgg
     5941 cttgtccttg acgacgtagg cgtagccgaa gcacatgtgg acgatggtcg gcgccgcgag
     6001 gccggcgagc gcgcgatcga tcgcctcgac gccgtagcgc cgcgccttgt cggggtgggc
     6061 ctggaggtac ggctcgtcga gctggatgaa gtcggcgccc gccgcgacga gatcgcatgc
     6121 ctcctggtgg accgcgtcgg cgtagtccat cgcgagcgcg cgctcgtcgc cgtagtgctc
     6181 gtccttggcg agctgggtca tggtgaacgg ccccggcagc gtgatcttga tcggccggcg
     6241 ggtgtggcgg cggaggaact cggcgtcgcg ctgctcgacc gggcgcagcc gctggacctt
     6301 gccgaccacg cgcggcatgg ggacctgctt gccgctgcgg ctctccacga ggccgggctg
     6361 gtcgatgtcg atgccggaca gcgcggtggc gaaccggttg aagtagctct cgcgccggat
     6421 ctcgccgtcg gtcaggatgt cgagcccggc gcgctccatg tcggcgatcg ccagccgggt
     6481 cgcgtcgtcc tgcgccgcct cgagg
//
LOCUS       DASVOR010000851         1947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_505078,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000851 DASVOR010000000
VERSION     DASVOR010000851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505078"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF516_16705"
     CDS             <1..656
                     /locus_tag="VF516_16705"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8109375.1"
                     /translation="RAAYGDSLDQLLVKCAGDPGCVARIGQRIGAAEVILVGVSELGD
                     VILTMQRIDVAGREITGRVADSLAPGSAPTDDQILGYTNRLLPPSDFLRFGVIDIVAN
                     LPGAAVTIGGAARGLTPIEALKLPAPASYDIRIEKAGYVPFTTRVALPPDGEIKVEAE
                     LVWARRGAPAWYQHWYVLAAAGVIVAGAGGTALYFATRTTTPAPPGDGTLHVTGSIH"
     gene            complement(637..1092)
                     /gene="rimI"
                     /locus_tag="VF516_16710"
     CDS             complement(637..1092)
                     /gene="rimI"
                     /locus_tag="VF516_16710"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEX8109376.1"
                     /translation="MIIAPATPADLDAIDEIERHSFPQPWPRAAFEAELTRPVARLDV
                     ARAPRVIGFCNYWIVPEPGANAGEVHIHTIATHPDHRRAGVGAALLDRALAEGRAAHC
                     NLATLEVRRGNAPAIALYRRAGFRVVHVRARYYQHNGEDALVMISEWSL"
     gene            complement(1089..1703)
                     /gene="yihA"
                     /locus_tag="VF516_16715"
     CDS             complement(1089..1703)
                     /gene="yihA"
                     /locus_tag="VF516_16715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HEX8109377.1"
                     /translation="MTRIAARFVTSAAQPSGFPPPSLPEIAVVGRSNVGKSSLINALV
                     GQPNLARTSRTPGRTRLINWFEIDARFYLVDLPGYGYAEVSQATRDSWRPLIEGYLAG
                     RQSLAGVLLLIDIRRGVQDEELDFAPWLAEREMPVVVALTKADKLPKNKRMLEVRRAR
                     AALGLARDPLAVSALGGDGIEPLWRAVVQLAAQPRQGKRPRQAR"
BASE COUNT          248 a          732 c          730 g          237 t
ORIGIN      
        1 cccgcgcggc gtacggcgac agcctcgacc agctgctggt caagtgcgcg ggcgaccccg
       61 gctgcgtcgc gcggatcggc cagcgcatcg gcgcggccga ggtgatcctc gtcggcgtca
      121 gcgagctcgg cgacgtgatc ctcacgatgc agcggatcga cgtcgccggc cgcgagatca
      181 ccggccgggt cgccgactcg ctcgccccgg gcagcgcgcc gaccgacgac cagatcctgg
      241 gctacaccaa ccggctgctg ccgccgagcg acttcctgcg gttcggcgtg atcgacatcg
      301 tcgccaacct gcccggggcg gcggtgacga tcggcggcgc ggcgcgcggg ctgaccccga
      361 tcgaggcgct caagctgccg gcgccggcga gctacgacat ccggatcgag aaggccggct
      421 acgtgccgtt caccacccgg gtcgcgctac cgcccgacgg cgagatcaag gtcgaggccg
      481 agctggtgtg ggcgcggcgc ggcgcgccgg cgtggtacca gcactggtac gtgctcgccg
      541 cggcgggcgt gatcgtcgcc ggcgcgggcg ggaccgcgct gtacttcgcc acgcgcacca
      601 ccacaccggc gccgccgggc gacggcacgc tccacgtcac aggctccatt cactgatcat
      661 cacgagcgcg tcctcgccgt tgtgctggta gtagcgcgcg cggacgtgga cgacgcggaa
      721 ccccgcgcgc cggtacagcg cgatcgccgg cgcgttgccg cggcggacct cgagcgtcgc
      781 gaggttgcag tgcgcggcgc ggccctcggc gagcgcgcgg tcgagcagcg cggcgccgac
      841 cccggcgcgc cggtgatcgg ggtgggtggc gatcgtgtgg atgtggacct cgccggcgtt
      901 cgcgccgggc tcggggacga tccagtagtt gcagaacccg atcacccggg gcgcgcgggc
      961 gacgtcgagc cgcgccaccg ggcgggtcag ctcggcctcg aacgccgcgc gcggccacgg
     1021 ctgagggaac gagtggcgct cgatctcgtc gatcgcgtcg agatcggcgg gcgtcgccgg
     1081 ggcgatgatc atctcgcctg ccgcgggcgc ttcccctgcc gcggctgggc ggcgagctgg
     1141 accaccgcgc gccacagcgg ctcgatcccg tcgccgccga gggccgacac cgcgagcgga
     1201 tcgcgcgcca ggccgagcgc ggcgcgggcg cggcggacct ccagcatccg cttgttcttg
     1261 ggcagcttgt cggccttggt cagcgcgacg acgaccggca tctcgcgctc ggcgagccac
     1321 ggcgcgaagt cgagctcctc gtcctgcacg ccgcggcgga tgtcgatcag cagcagcacg
     1381 ccggcgagac tctggcgccc ggcgaggtag ccctcgatca gcggccgcca gctgtcgcgc
     1441 gtcgcctggc tgacctcggc gtagccgtag cccggcagat cgaccaggta gaaccgcgcg
     1501 tcgatctcga accagttgat cagccgggtc cgccccggcg tgcgcgacgt gcgcgcgagg
     1561 ttgggctggc cgaccagcgc gttgatcagg ctcgacttgc cgacgttgga ccggccgacc
     1621 acggcgatct cgggcagcga cggcggcggg aagcccgacg gctgcgccgc cgaggtgaca
     1681 aagcgcgcgg cgatccgggt catggccggg gcgccatcgg atgcaccggc gcatccgggt
     1741 cgatcccacg gcgggctttg ccccccgccc cgccggcaga gccggcaggg tccccacccc
     1801 ccatctcgct gcgctcgatc ccacggggag cgttgtcccc cgccccgccg gcagagccgg
     1861 cagggtcccc actccccatc gcgctgcgcg cgatccgcgc agcggcgcgg ccgggcgccc
     1921 ggagaccacc cgcgcgcggg cccgcaa
//
LOCUS       DASVOR010000852        10867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_271196,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000852 DASVOR010000000
VERSION     DASVOR010000852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271196"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..251)
                     /locus_tag="VF516_16720"
     CDS             complement(69..251)
                     /locus_tag="VF516_16720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109378.1"
                     /translation="MARRTGDPLPVFKVGGIVRLNTNDLEDWLVRQRDRSMRMPAHAP
                     VLPATPGDDVTLRLIA"
     gene            689..2554
                     /locus_tag="VF516_16725"
     CDS             689..2554
                     /locus_tag="VF516_16725"
                     /inference="COORDINATES: protein motif:HMM:NF017400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase S55 domain-containing protein"
                     /protein_id="HEX8109379.1"
                     /translation="MRGFVRHCAAAGVLAYATIAYAQPTEIFPVSQVQRGQTGYGLTT
                     FAGTKPERFTFEVVSVVKNFLPKQDIILVKSDDPKMAVSGFWQGMSGSPLYIDDKLVC
                     AFSYGFRFNKVALGGCTPIEYMKHEADAVRRGKVVQPAGVAYKTVQPMAASLEDWRRL
                     TPTVDAAEAMAALGPVRKSWLLSAPLPSPVTRPPAVDDQVMTASVPLSIAGFSAPAFG
                     QLQKLFGDSNIVPMRAGGTSGGTGGKLEAGPTQFLPGAPLAVDLIRGDMSAVGICTVS
                     YVDGDKVLSCGHPIFQSGEFYAPVSTASIAAVVPSAQSAFLMGTSINEIGSLVQDRQA
                     AIVADTGLRSPTIPVDIAITSGTDKHVEKGNFHVEILSNKFLTPALAGAAVMNAINYY
                     LPDKDNVTARVESRVQIAGQEPINFVDYVYANDGAASVMGAVRGLRVLVPLLLNPYAP
                     VSVERVELKVDLRFEANYGEIKEIKIPGSELSVGHNVLQVLMSTWDGKDITEEVPVDV
                     PASLAGGIVQVEVCAGDAAKLDAPPPVDLPSLLRAFRSLLPGNIWAVTLYPADEGVAL
                     DGKLVRDLPQSALDKLHPQSHTQRAQLYKPIARTKSPSRRVVNGTSSTLVHVRAR"
     gene            2584..4797
                     /locus_tag="VF516_16730"
     CDS             2584..4797
                     /locus_tag="VF516_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109380.1"
                     /translation="MKAIDLEGAFPPPLSRQSREIHPSARRVARSIPLSIACSALLLA
                     LPAGAVVTATWGVETYPQWDAGDATSAFITSAGELRPGWDIKRTALEGDAVWSSLHLA
                     DGSVLLGSDAGGAIYKLSGDAATRLVALPGAIAVVALAQTSDGAVWAGAMPGNKLWKI
                     DVAAGKATPTGPLGKDKDVETVWSLTAVGNTVYAGTGPAGKLFAVTGGVAKEVFATDD
                     KRITALTVAGDGAVWLGTSDRALVFRFDPKLGEARAMADFAGNEVSSLAAFQGGVIAA
                     ANDLVEQPPPTAKTPAQVEAAEKPTAAKGQVAKPPEVGSRPGADKDPVPVTDLGRKGA
                     KKGKGALFRIGKDGRLDQLHALTQTYFTSIAVGPDGAIYAGAADKGRIYMVDTDDSIA
                     TAFDVDERAVSQLWFDHGLLSFATDDASAAYRATGRASQARYASDVYDAKAVSRFGKL
                     TWAASGKVKLETRSGNTAKPGVGWSKWQVPTAIGKIGGGTEGGKIASPAGRYLQFRIA
                     LEDSAARVRRVTSYYVPQNQPTTVQDVSVEIANKESLPTLKDSAAKPRSPTLRVKWKI
                     ENPDGDETSYTLEARREGEANWRPIQTGKAPLTTTSWEWNTETYPDGWYRLRVTASDA
                     AANSPDRALTSSQTTTMFAIDNTRPVIDGLTVTHPRAQARATDALSTIAEMAYSVDDG
                     PWQLGASADGLFDDLTEDLRIELPAGLARGTHTLAVRVADAAGNIGSTSATFVVK"
     gene            complement(4822..6153)
                     /locus_tag="VF516_16735"
     CDS             complement(4822..6153)
                     /locus_tag="VF516_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109381.1"
                     /translation="MTRLSATAALLFVVGCSYGDNDFPVPDHGDPPALTGPLLGFETA
                     AVTGANASVLPAGFTGPLETSCAQDVTRGFIAELIEHGFNNPQVAYEWAPVAPGPTSA
                     PPIRQPEFFASGTVTGLDRSGLDFRPSHPFGFDTTWDFRVDAPFLGLVHNRPGDPNDG
                     DAIHAELESGLYPDAQFGIVPTAGDRVAMKGAWILDCGHPPYEAEIHPPTFLAVARAE
                     GTDTVALAFANPYRSSQLYGLPEETTVFSDDTRFSAMDASPFTRALTIEVIQAAFGEI
                     TQFELHALLEATHLDPVTWYVCAPPRPSPSARLHYSFRFVARRGVTISAATRGDSGCL
                     EFHAEAGSGYRPFVPDRLDHPWTWAEINSEASSQAGSDVDVRQAIVDTLKQQGLPADG
                     VALRPETATVVDEYMPLQPRGGANADGPIAVVGSADDQPFPFYGRVRVGWK"
     gene            7143..9842
                     /locus_tag="VF516_16740"
     CDS             7143..9842
                     /locus_tag="VF516_16740"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8109382.1"
                     /translation="MEAPLSGATVTIVGTGIATTTDEAGRFQLAAPSGRVVLRAEVSG
                     FRAVERPLTVTAGKTSEVDLPMTLDQLLTEVVVVVGSRTPRTNVETTAPVDVVTAEEI
                     SHVGKTETGRVLSTLAPSYISTPQTIADGTDHVDPAALRGLGPDQVLVLVNGKRRHKS
                     ALLNVNGTYGRGTVGTDLNAIPVDSIKRIEVLRDGAASQYGSDAIAGVINIVTKDYTD
                     LLDVTTMTGETGSRDGFQMKTGANYGFKIGKKGFLNITGDFLKKNPTNRAGQYNGFIF
                     VPAPTAPAGSPPGTPPDPAAVVAAQMADQAELTRRGMTRDDFRMREGEAGEQSAMGGY
                     NLEIPIDESTNFYSFGDLGHRNGDAAGFNRFPNATNQVVLALYPNGFLPEIQSNINDL
                     EVTAGVKRKGDWTIDASITHGQNSFQFNVEHSNNASLGALSPTSFDAGTLSSQQTLAD
                     LDLLRKIDTGFLKSLAFVAGSEVRTESYKITPGDDASWEFGPNNQWANDPTKVVKTSD
                     GKVPVPGAQVFPGFQPSNAVDRTRNNVGVYAGLESEIVKSLNADIGGRYENYSDFGNS
                     FTGKAALRVPLGSALAIRGAVSTGFRAPSLQQLWFNNTSTVFINDPMTGMLVPNQTLT
                     LNNANPIVRNAFGIPALRQETSQNASAGLTFRPLDNLSITADGYFVRLKNRITLTNAF
                     QTSTPGAAGMVIRQILAPFPGVTSAQFFANAVDTDTMGADIVGDYAVNTGAGTLLFSS
                     ALNLTKTEVKDVHAPKQLSDTFASVTPADFANLYFDRLARNRLEDSVPHVKGYASLRY
                     NFRQLTALVRADYYGQVRYKPDLVSDSQTFSPKTLFDAELGYQFTKNMQLTVGGDNIF
                     NTFPDQNTKANNLFNNRLIYNRNVSQFGLNGGFYYGKLELTFF"
     gene            9898..>10867
                     /locus_tag="VF516_16745"
     CDS             9898..>10867
                     /locus_tag="VF516_16745"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109383.1"
                     /translation="MKTAEPTAPEEFGPYLVYERLGIGGMATVHRAKKRGIAGFERGV
                     ALKRMLPHLADDAEFIHSFVREAKLASLLVHPNIAQIYDFGRVGSVYYIAMEHVDGFD
                     VRKMLRYSNRHKDPLPLPVVLSILGELCDALEYAHTFVDEHGAPQGIVHRDVSPSNLI
                     VAHSGHLKVIDFGIAKANARQLHTESGRVKGKLGYMSPEAVSGRAFGPMSDVFSAGVV
                     AHELLTAHPLFSARTDYDTLIRIHEAEIPPPSRRNPSVPAALDEIVLAALHREPDRRL
                     QSAGAFRQGLDFVAEQAGIRFSARDITEWRARIATSDDPWGSRSSLP"
BASE COUNT         1831 a         3832 c         3651 g         1553 t
ORIGIN      
        1 ggacagcgga cgactcatga gggccggttc ttagtgcgaa ccgcgcgtca tgtccacccc
       61 gccggaggct aagcgatcaa ccgaagcgtg acgtcatcac ccggtgtcgc ggggagcacc
      121 ggagcatgcg ccggcatgcg catcgagcgg tcgcgctggc ggaccagcca gtcctcgagg
      181 tcgttggtgt tgaggcgaac gatcccgccg accttgaaca ccggcagcgg atcgccggtg
      241 cggcgtgcca tgtacctgca ccaacgctcg gagcgaccca gggccgtagc gattcccttc
      301 cacgtttcca cgaaattgga catgccatta cctccgtgag ccgcttcggt ttgcagaggc
      361 tgtgccgagg tccgaccggt ctgctaagtc gtcggagaga ctggcccgtg gatgtcaagt
      421 ccatacatcg ccggagatgg aagcccacca acgaccgacg caaaacctga cacagttgtc
      481 gcgaacttgc cccaccgccc ccagtgctcc tcgacgcaac gtgagccgcc gaccattcct
      541 ggcaaattcc cggaatgtca ggctgctccg ggcagtcgcg gcggcgcgac cgggccgcga
      601 cacgaggcgt gttccggtcc ccggcatctg cgcccggctc agggttggtt gtggcggcac
      661 ctgcacaggg ccgctatgct cgctgcacat gcgcggattt gtgcggcact gcgccgctgc
      721 cggggtgctc gcgtacgcca cgatcgcgta cgcgcagccg accgagatct ttccggtgtc
      781 ccaggtacag cgaggacaga ccgggtacgg cttgacaacc ttcgccggga ccaaacccga
      841 gcgcttcacc ttcgaggtcg tctcggtggt gaagaacttc ctccccaagc aggacatcat
      901 cctcgtcaag tcggacgacc ccaagatggc ggtgtccggg ttctggcagg gcatgtccgg
      961 aagcccgctg tacatcgacg acaagctggt gtgcgcgttc tcctacgggt tccggttcaa
     1021 caaggtcgcg ctcggcggct gcacgccgat cgagtacatg aagcacgagg ccgatgccgt
     1081 gcgccgcggc aaggtggtcc agccggcggg cgtcgcctac aagaccgtgc agccgatggc
     1141 ggcctcgctg gaggactggc ggcggctgac cccgacggtc gacgccgcgg aggcgatggc
     1201 ggcgctcggc ccggtgcgca agagctggct gctgtcggcg ccgctgccgt cgccggtgac
     1261 ccgcccgccg gcggtcgacg accaggtgat gaccgcgtcg gtgccgctgt cgatcgccgg
     1321 gttctcggcg cccgcgttcg gccagctgca gaagctgttc ggcgattcca acatcgtgcc
     1381 gatgcgcgcc ggcggaacgt cggggggcac cgggggcaag ctcgaggccg gcccgaccca
     1441 gttcctgccg ggcgcgccgc tcgcggtcga cctgatccgc ggcgacatga gcgcggtcgg
     1501 gatctgcacg gtgtcgtacg tcgacggcga caaggtgctg tcgtgcggcc acccgatctt
     1561 ccagtccggc gagttctacg cgccggtgtc gaccgcgagc atcgcggcgg tggtgccgtc
     1621 ggcgcagagc gcgttcctga tggggacctc gatcaacgag atcggctcgc tggtccagga
     1681 ccgccaggcg gcgatcgtcg ccgacaccgg gctgcgctcg ccgacgatcc cggtcgacat
     1741 cgcgatcacg agcggcaccg acaagcacgt cgagaagggc aacttccacg tcgagatcct
     1801 gagcaacaag ttcctgaccc cggcgctcgc cggcgccgcg gtgatgaacg cgatcaacta
     1861 ctacctgccg gacaaggaca acgtcaccgc gcgggtcgag tcgcgggtcc agatcgccgg
     1921 ccaggagccg atcaacttcg tcgactacgt gtacgccaac gacggcgcgg ccagcgtgat
     1981 gggcgcggtg cgcgggctgc gcgtgctggt cccgctgctg ctcaacccgt acgcgccggt
     2041 gtcggtcgag cgggtcgagc tcaaggtcga cctccggttc gaggccaact acggcgagat
     2101 caaggagatc aagatcccgg gctccgagct gtcggtcggc cacaacgtgc tccaggtgct
     2161 gatgagcacc tgggatggca aggacatcac cgaggaggtc ccggtcgacg tgccggccag
     2221 cctggccggc gggatcgtcc aggtcgaggt ctgcgccggc gacgccgcca agctcgacgc
     2281 gccgccgccg gtcgacctgc cgtcgctgct ccgcgcgttc cgctcgctgc tgcccggcaa
     2341 catctgggcg gtgacgctgt accccgccga cgagggcgtc gcgctcgacg gcaagctggt
     2401 gcgcgacctg ccgcagagcg cgctcgacaa gctccacccg cagagccaca cccagcgcgc
     2461 ccagctgtac aagccgatcg cgcgcaccaa gtctccctcc aggcgcgtcg tcaacggcac
     2521 gtcgtcgacg ctggtccacg tccgcgctcg ctgatccgag cacttgcagg agatctccgt
     2581 cccgtgaaag ccatcgatct cgagggggcc ttcccgcccc ctctttcgcg gcagagccgc
     2641 gaaattcacc cctccgcgcg tcgcgtcgcg cggtcgatcc ctctgtccat cgcgtgcagc
     2701 gccctgctgc tcgcgctccc cgccggcgcc gtcgtcaccg cgacctgggg cgtcgagacc
     2761 tacccgcaat gggacgccgg tgacgcgacc agcgcgttca tcacctcggc cggcgagctc
     2821 cgccccggct gggacatcaa gcgcaccgcg ctcgagggcg acgcggtgtg gagctcgctg
     2881 catctcgccg acggcagcgt gctgctcggc tcggacgcgg gcggcgcgat ctacaagctg
     2941 tcgggcgacg ccgcgacccg cctggtcgcg ctgcccggcg cgatcgccgt cgtcgcgctc
     3001 gcccagacca gcgacggcgc ggtgtgggcc ggcgcgatgc cgggcaacaa gctgtggaag
     3061 atcgacgtcg ccgccggcaa ggcgacgccg accggcccgc tcggcaagga caaggacgtc
     3121 gagaccgtgt ggtcgctgac cgcggtcggc aacacggtct atgccgggac cggcccggcg
     3181 ggcaagctgt tcgcggtcac cggcggggtc gccaaggagg tgttcgccac cgacgacaag
     3241 cggatcaccg cgctgaccgt ggccggcgac ggcgcggtgt ggctcggcac cagcgatcgc
     3301 gcgctggtgt tccggttcga tcccaagctc ggcgaggcgc gcgcgatggc cgacttcgcg
     3361 ggcaacgagg tgtcgtcgct cgcggcgttc cagggcggcg tgatcgccgc ggccaacgac
     3421 ctggtcgagc agccgccgcc gaccgccaag accccggcgc aggtcgaggc cgccgagaag
     3481 cccaccgccg ccaagggcca ggtcgccaag cctcccgagg tcggctccag gccgggcgcc
     3541 gacaaggatc cggtcccggt gaccgacctc ggccgcaagg gcgccaagaa gggcaagggc
     3601 gcgctgttcc ggatcgggaa ggacggccgg ctcgaccagc tgcacgcgct gacccagacc
     3661 tacttcacgt cgatcgcggt cggccccgat ggcgcgatct acgccggcgc cgccgacaag
     3721 ggccggatct acatggtcga caccgacgac tcgatcgcca ccgcgttcga cgtcgacgag
     3781 cgcgcggtgt cgcagctgtg gttcgatcac ggcctgttgt cgttcgccac cgacgacgcg
     3841 tcggcggcgt atcgcgcgac cggccgggcc tcgcaggcgc gctacgccag cgacgtgtac
     3901 gacgccaagg cggtgtcgcg gttcggcaag ctgacctggg cggcgtccgg caaggtcaag
     3961 ctcgagaccc gcagcggcaa caccgccaag cccggcgtcg gctggagcaa gtggcaggtg
     4021 ccgaccgcga tcggcaagat cggcggcggc accgagggcg gcaagatcgc cagcccggcc
     4081 ggccgctacc tgcagttccg gatcgcgctc gaggacagcg cggcccgggt ccgccgggtc
     4141 accagctact acgtgccgca gaaccagccg accacggtgc aggacgtctc ggtcgagatc
     4201 gcgaacaagg agtcgctgcc cacgctcaag gactcggcgg ccaagccgcg cagcccgacg
     4261 ctgcgggtca agtggaagat cgagaacccc gacggcgacg agaccagcta cacgctcgag
     4321 gcccggcgcg agggcgaggc caactggcgg ccgatccaga ccggcaaggc gccgctgacc
     4381 acgacgtcgt gggagtggaa caccgagacc taccccgacg gctggtaccg gctccgggtc
     4441 accgcgtccg acgccgcggc gaactcgccc gaccgcgcgc tgacgtcgag ccagaccacg
     4501 acgatgttcg cgatcgacaa cacccggccg gtgatcgacg gcctcacggt gacccatccg
     4561 cgtgcgcagg cgcgcgccac cgacgcgctc agcacgatcg ccgagatggc gtactcggtc
     4621 gacgacgggc cgtggcagct cggcgcctcg gcggacgggc tgttcgacga cctcaccgag
     4681 gacctgcgga tcgagctgcc ggcggggctg gcgcgcggga cccacacgct cgccgtccgg
     4741 gtcgcggacg cggccggcaa catcggctcg acgtcggcaa cgttcgtcgt gaagtagccg
     4801 cgcgccgacc gcgggtggcc gtcacttcca gccgacgcgc acgcggccgt agaacgggaa
     4861 cggctgatcg tcggccgagc cgaccacggc gatcgggccg tctgcgttcg ctccaccgcg
     4921 cggctggagc ggcatgtact cgtcgacgac ggtggcggtc tcggggcgaa gcgccacgcc
     4981 atccgcgggg agcccctgct gcttcagggt atcgacgatc gcctgccgca cgtcgacatc
     5041 gctgccggct tgcgagctgg cctcgctgtt gatctcggcc caggtccacg ggtggtcgag
     5101 gcgatccggg acgaacggcc ggtagccgct gccggcctcg gcgtgaaact cgaggcagcc
     5161 gctgtcgccc cgggtggccg ccgagatcgt gacgcccctg cgtgcgacga agcggaagct
     5221 gtagtgcagc cgcgccgacg gcgacgggcg cggtggcgcg cacacgtacc aggtgaccgg
     5281 atcgaggtgg gtggcctcga gcagggcgtg aagctcgaac tgcgtgatct cgccgaacgc
     5341 agcctggatc acctcgatcg tgagcgcgcg ggtgaacggg ctggcgtcca tcgccgagaa
     5401 ccgcgtgtcg tcggagaaca ccgtggtctc ctcggggagg ccgtagagct gcgagctgcg
     5461 gtacgggttg gcgaacgcca gcgcgaccgt gtcggtgccc tcggcgcgcg ccacggccaa
     5521 aaacgtcggt gggtggatct cggcctcgta gggcgggtgc ccgcagtcga ggatccaggc
     5581 gcccttcatc gcgacgcgat cgccggccgt cggcacgatg ccaaactgcg cgtcggggta
     5641 cagcccgctc tccagctcgg cgtggatcgc gtcgccatcg ttgggatcgc cgggccggtt
     5701 atggaccagg ccgagaaacg gggcgtcgac ccggaagtcc cacgtggtgt cgaacccgaa
     5761 cgggtgcgac ggccggaaat cgaggccgga gcggtcgagc ccggtcacgg tgcccgacgc
     5821 gaagaactcc ggctgccgga tggggggggc agaggtcggg ccgggggcga ccggcgccca
     5881 ctcgtaggcg acctgggggt tgttgaagcc atgctcgatg agctcggcga tgaatccgcg
     5941 ggtcacgtcc tgggcgcacg acgtctcgag cggcccggtg aagccggccg gcagcacgga
     6001 cgcgttcgcg ccggtgaccg cggcggtctc gaagccgagc agcgggccgg tcagcgcggg
     6061 tgggtcgccg tgatcgggga cgggaaaatc gttgtccccg tagctacagc ccacgacgaa
     6121 gaggagcgcg gcggtcgcag agagcctggt catgatgaat gatcttccgg gtgaccgtcc
     6181 gagacaagcg cgcagccgga acccgccgga tcaggtgtcg cggcgcagcg ttgggcggta
     6241 ctcgtgaagc cgcgagcggg ccgccaggcg cccatgcccg gcgtccgcat cggatcgacc
     6301 gcctcggctg aaccgcggtc cagggcgccc gccgcggtgg ccagccacgg acgagccgtc
     6361 aacagatacc gcaccgacat gggtcggcgc cgccagcgtc ccagctgcga caggcgattc
     6421 accggaggtg cccactcgcg tcatcactcg ctttcatgga tggattccag atctcagatc
     6481 acgaaatagc tatcagaatc ctgttctgcg gtgcggtctg ttgcgcttga taacgtacga
     6541 acgctgacaa cgaaatctcc agacgaccgg cctgagctga ctttcgccgt ccgcaactcg
     6601 ccgcgggcac agcgatccgg cgagcacatg tcagccgcgg tcatcgcctc tccggagctg
     6661 agggtttttc aggcacatgg ctgcgagcga tggtgatcgc gaggtgggtt cgcgcggtcg
     6721 cacagcggct catcctggac tacaggtccg gagctcgatc cggatgcgat ggaccggtgt
     6781 catcagggtt tttacgacgt gagccatcga accgaaattt agtgtaaata aaatacagct
     6841 tcatatctgg aactccctgc ttgcattgca ggtccatgtt gatactgtcg tgagccacgg
     6901 gaggtcctgt gcattcgaga ttgcattcac tattaccgta tctctcggcg gttatccttg
     6961 gtattggccc tggtacccag gttgcatcgg cacagccggc accgagcgat acaccccccg
     7021 cagatccgca aaccgatcct tcaaatacga atcagccggc gagcgaacct ccgtcgggtg
     7081 aggtcaaggc gtcgccctcc ggcgagaccg gcacgatctc gggcacgatc cggtcgatcg
     7141 acatggaggc gccgctgtcg ggagccacgg tgacgatcgt cggcaccggg atcgcgacga
     7201 ccaccgacga ggcggggcgc ttccagctgg ccgcgccctc cggacgggtc gtgctgcgcg
     7261 ccgaggtcag cgggttccgc gcggtcgagc ggccgctgac ggtcacggcc ggcaagacct
     7321 ccgaggtcga cctgccgatg acgctcgacc agctgctcac cgaggtcgtg gtcgtcgtcg
     7381 gttcgcgaac gccgcgcacc aacgtcgaga ccaccgcccc ggtcgacgtg gtgaccgccg
     7441 aggagatctc ccacgtcggc aagaccgaga ccggccgcgt cctgtcgacg ctggcgccgt
     7501 cgtacatctc gacgccacag accatcgccg acggcaccga ccacgtcgac cccgccgcgc
     7561 tgcgcggcct ggggcccgat caggtgctcg tcctggtcaa cggcaagcgc cgccacaaga
     7621 gcgcgctgct caacgtcaac ggcacctacg gccgcggcac ggtcggcacc gacctcaacg
     7681 cgatcccggt ggactcgatc aagcggatcg aggtcctgcg cgacggcgcc gcctcgcagt
     7741 acggctcgga cgcgatcgcc ggcgtgatca acatcgtgac caaggactac accgacctgc
     7801 tcgacgtcac gacgatgacc ggggagaccg gctcgcgtga cggcttccag atgaagaccg
     7861 gcgcgaacta cgggttcaag atcggcaaga agggcttcct gaacatcacc ggcgacttcc
     7921 tcaagaagaa cccgaccaac cgcgccggtc agtacaacgg cttcatcttc gtgcccgcgc
     7981 ccaccgcgcc cgccggcagc ccgcccggca ccccacccga cccggccgcg gtagtcgccg
     8041 cgcagatggc ggaccaggcg gagctcaccc ggcgcggcat gacccgcgac gacttccgga
     8101 tgcgggaagg cgaggccggc gagcagagcg ccatgggcgg ttacaacctg gagatcccga
     8161 tcgacgagtc gacgaacttc tacagcttcg gcgacctcgg ccaccgcaac ggcgacgcgg
     8221 ccggattcaa ccggttcccg aacgcgacca accaggtcgt cctggcgctc taccccaacg
     8281 gcttcttgcc ggagatccaa agcaacatca acgacctcga ggtgacggcc ggcgtcaagc
     8341 gcaagggcga ctggaccatc gatgccagca tcacccacgg ccagaactcg ttccagttca
     8401 acgtcgagca ctcgaacaac gcctcgctcg gcgcgctcag cccgacgtcg ttcgatgcgg
     8461 gcacgctgag cagccagcag accctcgccg acctcgatct gctgcgcaag atcgacaccg
     8521 gcttcctcaa gtccctggcg ttcgtcgccg gcagcgaggt gcgcaccgag agctacaaga
     8581 tcacgccggg cgacgacgcg tcctgggagt tcggtcccaa caaccaatgg gccaacgatc
     8641 cgacgaaggt ggtgaagacc tccgacggca aggtcccggt gcccggcgcc caggtcttcc
     8701 ccggtttcca gccgagcaac gcggtcgacc gcacccgcaa caacgtcggc gtgtacgccg
     8761 gcctcgagtc cgagatcgtc aagagcctca acgcggacat cggcggccgc tacgagaact
     8821 acagcgactt cggcaactcg ttcaccggaa aggccgcgct gcgcgtgccg ctgggcagcg
     8881 cgctcgcgat ccgcggcgcg gtgagcacgg gattccgcgc gccgtcgctg cagcagctgt
     8941 ggttcaacaa cacgtcgacc gtcttcatca acgaccccat gaccggcatg ctggtgccca
     9001 accagacgct gaccctcaac aacgccaacc ccatcgtccg caacgcgttc gggatcccgg
     9061 cgctcaggca ggagacctcg cagaacgcca gcgccggcct cacgttccgt ccgctcgaca
     9121 acctgtcgat cactgccgac gggtacttcg tccggctcaa gaaccggatc acgctgacca
     9181 acgcgttcca gaccagcaca cccggcgcgg cggggatggt gatccggcag atcctcgcgc
     9241 cgtttccagg cgtgaccagc gcgcagttct tcgccaacgc agtcgacacc gacacgatgg
     9301 gtgcagacat cgtcggcgac tacgcggtca acaccggcgc cggcacgctg ctgttcagca
     9361 gcgcgctgaa cctgaccaag accgaggtca aggacgtcca cgccccgaag cagctgtcgg
     9421 atacgttcgc gagcgtcact ccggcggact ttgccaacct ctacttcgat cggctggcgc
     9481 gcaaccggct cgaggactcc gtgccacacg tcaaggggta cgcgtcgctg cgctacaact
     9541 tccggcagct gacggccctg gtccgcgccg actactacgg ccaggtccgc tacaagcccg
     9601 acctggtttc cgacagccag accttcagcc ccaagacgct gttcgacgcc gagctcggct
     9661 accagttcac caagaacatg cagctcaccg tcggcggaga caacatcttc aacaccttcc
     9721 cggaccagaa caccaaggcc aacaatctgt tcaacaaccg cctcatctac aaccggaacg
     9781 tctcgcagtt cggcctgaac ggtgggttct actacggcaa gctcgagctg acgttcttct
     9841 gatccgcgcc gcagccccgc cggatgagcc ccacaggtgc ccgcgcagga acgtgccgtg
     9901 aagaccgctg agcccaccgc tcccgaagaa ttcggcccgt acctcgtgta cgagcggctc
     9961 gggatcggcg gcatggcgac ggtccaccgc gccaagaagc gcgggatcgc cgggttcgag
    10021 cgcggcgtcg cgctcaagcg gatgctgccg cacctggccg acgacgccga gttcatccac
    10081 tcgttcgtgc gcgaggccaa gctcgcgtcg ctgctggtcc accccaacat cgcgcagatc
    10141 tacgacttcg ggcgggtcgg ctcggtctac tacatcgcga tggagcacgt cgacgggttc
    10201 gacgtgcgca agatgctgcg ctacagcaac cgccacaagg atccgctgcc gctccccgtg
    10261 gtgctgtcga tcctcggcga gctgtgcgac gcgctggagt acgcccacac gttcgtcgac
    10321 gagcacggcg cgccgcaggg catcgtgcac cgcgatgtct cgccgtccaa cctgatcgtc
    10381 gcgcacagcg ggcacctgaa ggtgatcgac ttcgggatcg ccaaggccaa cgcccgccag
    10441 ctccacaccg agagcggccg ggtcaagggc aagctcggct acatgtcgcc ggaggcggtc
    10501 agcggccgcg cgttcggccc gatgtccgac gtgttctccg ccggcgtggt cgcccacgag
    10561 ctgttgaccg cgcacccgct gttctcggcc aggaccgact acgacacgct gatccggatc
    10621 cacgaggccg agatcccgcc gccgtcgcgg cgcaacccga gcgtgccggc ggcgctcgac
    10681 gagatcgtgc tcgccgcgct ccaccgcgag cccgatcgcc ggctgcagtc cgccggggcg
    10741 ttccgccagg ggctcgactt cgtcgccgag caggccggca tccggttctc ggcgcgcgac
    10801 atcaccgagt ggcgggcccg gatcgccacc tccgacgatc cgtggggctc gcgcagctcg
    10861 ctgcccg
//
LOCUS       DASVOR010000853         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_505115,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000853 DASVOR010000000
VERSION     DASVOR010000853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505115"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..1388
                     /locus_tag="VF516_16750"
     CDS             189..1388
                     /locus_tag="VF516_16750"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX8109384.1"
                     /translation="MGELAPVSPRERVATLDVLRGFALLGVLLANLYSLFSFRFAIHE
                     PPDASIADVAARWFMVLVVRGRAQTLLTLLFGFGFAVQLLRARERGQRVLPVYLRRLL
                     ALFAIGWLHVLLLSWVDVTWGYALVALFLLPFVRVSDRTRLIAAAALTIIPAVVYAFP
                     AVGPAFHALLFDQPRATYVEAFAAAARSGDRIAILHAHVTLALLWTLGSNGVWYLPWM
                     LARFQLGLVAGARRWFDRDGADHLPLFRKLLVGGAVGTVVGWVPEALIAAHLFDPAGH
                     GLAVAVAVAIVSRLALLAQVAMYVAIVVRVMQRPAWRRALAVIAPVGRMPLTTYLMQS
                     LICTSLFYGWGLAWRTPPPAACVGLGLAIFAVQVAIAHVWLRWFRFGPAEWLWRSIVY
                     WRPQPMR"
BASE COUNT          164 a          548 c          545 g          281 t
ORIGIN      
        1 accgcatgcg gagtgcagct ttacagctcc acgccgtttg agatcactca cgttcacaag
       61 cgatcacgcc atgttgacaa cagaagcaac gcgcaccgcg actcgcgtgc cgcttcacgg
      121 tccagctcga tcccgagcca cagtattgtg agcatggcat tccgcatgcg aaccggtatt
      181 cttgtctcgt gggggaactg gcgccggtat cgcctcgtga gcgcgtcgcg acgctcgacg
      241 tgctgcgtgg ctttgcattg ctcggagtcc tgctcgcgaa cctctactcg ctgttcagct
      301 tccggttcgc gatccacgag ccgcccgacg ccagcatcgc cgacgtggcc gcgcgctggt
      361 tcatggtgct ggtggtgcgc ggccgggcgc agacgctctt gacgctgctg ttcggcttcg
      421 gcttcgccgt gcagctgctg cgcgcacgcg agcgcggcca gcgcgtgctg ccggtctacc
      481 tgcggcggct gctcgcgctg ttcgcgatcg gctggctgca cgtgctgctg ctgtcgtggg
      541 tcgacgtgac gtgggggtat gcgctcgtcg ccctgttcct gctcccgttc gtgcgcgtca
      601 gcgatcgcac ccggctgatc gccgccgcgg cgctgacgat catcccggcg gtggtctacg
      661 cgttccccgc ggtggggccg gcgttccacg ccctgctgtt cgatcagccc cgcgccacct
      721 acgtcgaggc gttcgccgcc gcggcgcgca gcggcgaccg gatcgcgatc ctgcacgcgc
      781 acgtgaccct cgcgctgctg tggacgctcg gcagcaacgg ggtgtggtac ctgccgtgga
      841 tgctcgcccg gttccagctc gggctggtcg ccggcgcccg gcgctggttc gaccgcgacg
      901 gcgccgacca cctcccgctg ttccgcaagc tgctggtcgg cggcgccgtc gggacggtgg
      961 tcgggtgggt gccggaagcg ctcatcgcgg cgcacctgtt cgatccggcc ggccacgggc
     1021 tggcggtggc cgtggccgtg gcgatcgtct cgcggctcgc gctgctcgcg caggtcgcga
     1081 tgtacgtcgc gatcgtcgtg cgggtgatgc agcgaccggc gtggcgccgc gcgctcgccg
     1141 tgatcgcgcc ggtcggccgc atgccgctga ccacgtacct gatgcagagc ctgatctgca
     1201 cgtcgctgtt ctacggctgg gggctcgcct ggcgcacgcc accgcccgcg gcgtgcgtcg
     1261 gcctcgggct cgcgatcttc gcggtccagg tcgcgatcgc gcacgtctgg ctgcgctggt
     1321 tccggttcgg cccggccgag tggctgtggc ggtcgatcgt ctactggcgg ccccagccga
     1381 tgcggtgacg cgccgatcag cgcgggctcg cctggtggat cgcgtcgagc tcctgccagc
     1441 gcgcgaacag ccgctcgacc tcggcgcgcg cggcgtcgag ccctgcgatc agcgccggga
     1501 cctcggtcgg ccggtccttg aacacggccg gatccgac
//
LOCUS       DASVOR010000854         3535 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_154677,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000854 DASVOR010000000
VERSION     DASVOR010000854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154677"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VF516_16755"
     CDS             complement(<1..529)
                     /locus_tag="VF516_16755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109385.1"
                     /translation="MRNTAELPADLLDGRLAPSEPEDASSATRIAHVAQLADRGDHPT
                     AAREAAGLIEAGIHDIRLIAFYLFGVFLDRGIGYLPALLGRATALIADDLAALRPGRR
                     KLQVVNSATAWLFEHMSAQLVFHARQRSATWESWAGEADAALPDAIASGCTKLTLALE
                     AVIDAPLAAVPLARVR"
     gene            complement(526..912)
                     /locus_tag="VF516_16760"
     CDS             complement(526..912)
                     /locus_tag="VF516_16760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HEX8109386.1"
                     /translation="MAIAVCTGATMMCSFGVAPSSLVVLPLQHVLTRTPSANITDFAP
                     VMNIPPFGACTSLANPTVASATAAALGVLTPMPCVPVTAGPWVPGSMKVMNGGPPAVD
                     NNCKLMCSWGGVIQVLNPGQTVVIVG"
     gene            complement(903..2684)
                     /locus_tag="VF516_16765"
     CDS             complement(903..2684)
                     /locus_tag="VF516_16765"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8109387.1"
                     /translation="MAQSISDPAFAPAFALETPAVHGSPVLGWVAESQTAPARFLAAL
                     ESQARDLGVAPAKTTAAGADLYHDLVARHTGRGGRALRCHVRAALSGVGHWEDLTFDE
                     LHARCTYRAAWWAAQGIGPGSSLCVIFPVGVEFAVSLLAALRLGAAVSWIDPQGPDFV
                     TTRLAALAPEHVATSPFLARRLGPFAKLVLPPDALAPLPETYSHTYAPGEVCLQLFSP
                     LHAPGAAVAVTAGAAYAGALRDGLVCFALRPGDLLAAPGFHALQHQPAMLFAAWIVGA
                     AYLHMDEADAVRDPALLDVPLRTLGVSPRVRDAYLQARRGHRPPWAHIVRNPEEPCDW
                     EAWRAFLEGCDLGELPISNLVADAAAAGALLSSPRRPGKHALAYLQSVMPAPGRPWQL
                     LDFTRSGQPAAADAGVYARLGEGKKPKPLDPQYLVLARRGAEYLYGGTLEPRRCGRLF
                     PVDEVLAACARAPFLDGAAVVPVVSGGSASEARFVLVGFTGDEPRAQFDAALDRRCAE
                     LRARIAAALGDDAVPDQVVLFALYARRANGAIDQAWVQAQYLVGLLCRQAQAPVFHRL
                     TALRRSMRAAAAPPGPHPGPDKESPWP"
     gene            3022..>3535
                     /locus_tag="VF516_16770"
     CDS             3022..>3535
                     /locus_tag="VF516_16770"
                     /inference="COORDINATES: protein motif:HMM:NF017409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109388.1"
                     /translation="MKKVKLPMRFLVLGDFTGASDTPLDEREVQSILPGMQLKNFMSD
                     LDVTAPIEPRKLQQQLKGSLTGHITARFKKQPDPKATTADLLVTGTGKVRGAGKDNGL
                     GDFEGTVAIKGELKGVPLKDGVPTVTSATLRIAGKVRGDIDQGGITGTVQGSFPLTVA
                     AAPSSTSTDNT"
BASE COUNT          573 a         1209 c         1319 g          434 t
ORIGIN      
        1 ggcggacgcg ggccagcggc accgccgcga gtggcgcgtc gatcacggcc tcgagcgcga
       61 gcgtgagctt ggtgcatccg gaggcgatgg cgtcgggcag cgcggcgtcg gcctcgcccg
      121 cccaggactc ccaggtggcg ctgcgctgcc tggcgtgaaa cacgagctgg gccgacatgt
      181 gctcgaacag ccaggcggtg gcgctgttga cgacctggag cttgcggcgg ccgggccgga
      241 gcgcggcgag atcgtcggcg atcagcgccg tcgcgcgccc gagcagcgcc gggaggtagc
      301 caataccgcg gtccaggaac acgccgaaca ggtagaaggc gatcaaccgg atgtcgtgga
      361 tcccggcctc gatcaggccg gcggcctccc gtgcggccgt ggggtggtcg ccccggtcgg
      421 cgagctgcgc gacgtgcgcg atccgcgtgg cggacgaggc gtcctcgggc tcggacgggg
      481 cgagccgtcc gtcgaggagg tcggccggta gctcggcggt gttcctcatc cgacgatcac
      541 cacggtctgg ccggggttga gcacctggat gacgccgccc cagctgcaca tcagcttgca
      601 gttattgtcg acggccggtg gcccgccgtt catgaccttc atgctgccgg gcacccaggg
      661 ccccgccgtc accggcacgc agggcatcgg cgtcagcacc cccagcgccg ccgcggtcgc
      721 cgaggccacg gtcggattgg cgagcgacgt gcacgcgccg aacggcggga tgttcatgac
      781 gggggcgaag tcggtgatgt tggccgaggg cgtcctggtg aggacgtgct ggagcggcag
      841 caccacgagg ctggacggcg ccacgccgaa gctgcacatc atcgtcgcgc cggtacacac
      901 ggctatggcc acggggactc cttgtccgga ccggggtgcg ggccgggggg cgccgccgcg
      961 gcgcgcatcg atcgccgcag ggcggtcagt cggtggaaga ccggggcctg cgcctggcgg
     1021 cacagcaggc cgacgagata ctgcgcctgg acccacgcct gatcgatcgc gccatttgcc
     1081 cggcgcgcgt acagcgcgaa caggacgacc tggtccggga ccgcgtcgtc accgagcgcg
     1141 gcggcgatgc gcgcgcgcag ctcggcgcag cggcgatcga gcgccgcgtc gaattgggcg
     1201 cgcggctcgt ccccggtgaa gccgaccagc acgaaccgcg cctccgaggc ggacccgccg
     1261 ctgaccaccg gaaccacggc ggcaccgtcg aggaacgggg cgcgcgcaca ggcggcgagc
     1321 acctcgtcca ccgggaacag ccggccgcac cggcgcggct cgagcgtgcc gccgtagagg
     1381 tactcggcgc cgcggcgcgc caggaccaga tactgtggat cgagcggttt gggcttcttg
     1441 ccctctccca ggcgcgcgta caccccggca tccgccgccg cgggctggcc gctgcgcgtg
     1501 aagtcgagga gctgccacgg ccggccgggc gccggcatca cgctctggag atacgccagg
     1561 gcgtgcttgc cgggccgccg cggcgacgac agcaaggcgc cggcggcggc ggcgtccgcg
     1621 acgaggttcg agatcggcag ctcgcccagg tcgcagccct cgaggaacgc gcgccaggcc
     1681 tcccagtcgc acggctcctc ggggttgcgg acgatgtgcg cccagggggg ccggtgaccg
     1741 cggcgggcct ggaggtaggc gtcgcggacg cggggcgaga cgcccagggt ccgcagcggg
     1801 acgtccagca gcgccggatc gcggacggcg tccgcctcgt ccatatgaag gtacgcggcg
     1861 cccacgatcc aggccgcgaa cagcatcgcc ggctggtgct gcagcgcatg aaagccggga
     1921 gccgcgagca gatcgcccgg acgcagcgcg aagcagacca ggccgtcgcg cagcgccccg
     1981 gcatacgcgg ccccggcggt gaccgccacc gcggcacccg gcgcgtggag cggtgagaac
     2041 agctgcaggc acacctcgcc gggcgcgtag gtgtgggagt aggtctcggg cagcggcgcg
     2101 agcgcgtcgg gaggaagcac gagcttggcg aatggaccga gccggcgcgc gaggaacggg
     2161 ctggtggcga cgtgctcggg ggccagcgcg gccaggcgcg tggtcacgaa gtccggcccc
     2221 tgcggatcga tccacgacac cgcggctccg agccggagtg ccgccaggag cgacaccgca
     2281 aattcaactc ccacgggaaa tatcacgcag agggacgatc ccgggccgat gccttgtgcg
     2341 gcccaccagg cggcgcgata cgtgcaccga gcgtggagct cgtcgaacgt gagatcctcc
     2401 cagtgaccga cgccggagag ggcagcgcgc acgtggcagc gcagcgcccg cccgccgcgg
     2461 cccgtgtggc gagccacgag atcgtggtac aggtccgccc cggccgcggt ggtcttggcc
     2521 ggtgcgacgc cgaggtcgcg ggcctgggac tcgagcgcgg cgaggaacct cgcgggtgcc
     2581 gtctggctct cggcgaccca gccgagcacc gggctgccgt ggacggcggg cgtctcgagg
     2641 gcgaacgccg gagcgaatgc cggatcggaa atggactggg ccacgatgtg ctcgcgcggc
     2701 gaagcgccct gcgccgcttc gcccggtgtg gaaggtgatc gtaggcggcg cgttgcgcgg
     2761 gctcaagttt tcacgatcga ctgtacggat tcccggatga ctggacatgg agctcgcggc
     2821 gccggaggtc gccgaccaat tttttttgac cacctggata tttcacttca gagtcgcttt
     2881 cacgagcgcc tgcgcacatc aacccacgtc cattgcagcc aagcaggcag acacacactt
     2941 cgagaaagcg gggagcgaat gatcaagcca gtgcctcgat ccagattgaa catcacctat
     3001 cggacgaaga tcgaaggtac tttgaagaag gtgaagctcc cgatgcggtt cctcgtgctc
     3061 ggcgacttca ccggcgccag cgacacgccg ctcgacgagc gcgaggtgca gtcgatcctg
     3121 ccggggatgc agctcaagaa cttcatgtcc gacctcgacg tcaccgcacc gatcgagccc
     3181 cgtaagctgc agcagcagct caagggcagc ctgaccggcc acatcacggc caggttcaag
     3241 aagcagccgg accccaaggc gaccacggcc gacctgctgg tcaccgggac cggcaaggtc
     3301 cgcggcgcag gcaaggacaa cggactgggg gacttcgagg gcaccgtcgc gatcaaaggc
     3361 gagctcaagg gggtcccgct caaggacggc gtgcccacgg tcaccagcgc cacgctgcgc
     3421 atcgcgggca aggtgcgggg ggacatcgac cagggtggca tcaccggcac ggtgcagggc
     3481 tcgttcccgc tcaccgtggc cgccgcgccg tcgtccacca gcaccgacaa cacca
//
LOCUS       DASVOR010000855         6163 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_154683,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000855 DASVOR010000000
VERSION     DASVOR010000855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154683"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..796
                     /locus_tag="VF516_16775"
     CDS             <1..796
                     /locus_tag="VF516_16775"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEX8109389.1"
                     /translation="SPAAPASPGARGPAHIAPSVTLLAAPGIPATAPEVVDRVQQFYA
                     GIKQVTAQFRQSVTNNTFGSTKTSDGSVWIMKPGKMRWDYLEKRKDAVEVKKSFISNG
                     SSLYVVEHDNKQVVKKNLQQDLMPVAVSFLYGKGDLRAEFNAEIDKTGKYGEKDDIVL
                     RLTPKQASAQYKNLYLVVSPRDFHVSQSVIVDSSSNVNHFRFFAPDFEKPIKDSYFEF
                     DERSVKNYRVIDADASRDGLAGTGGLTPPAAPPAKPPPAKPPTTKP"
     gene            complement(803..1285)
                     /locus_tag="VF516_16780"
     CDS             complement(803..1285)
                     /locus_tag="VF516_16780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109390.1"
                     /translation="MANCKLCHAARLETESELIQTICAPCATKIGTTPMPPPRRPDVP
                     CPRCRGTRFIRVFPRERFVIDTASKWLGADFGSIGAMYAPMAATYDVSIVRHQVGVMI
                     GAPEVRGLDLTRPRGLIARYICSTCGFVELFCENPESIPIGSEYMSDVVDYGGSKPYR
                     "
     gene            1387..3036
                     /gene="gpmI"
                     /locus_tag="VF516_16785"
     CDS             1387..3036
                     /gene="gpmI"
                     /locus_tag="VF516_16785"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEX8109391.1"
                     /translation="MPAPSLRPHPRQPSPRGPVVLVVMDGVGRGAGDEADAVAIAATP
                     TLDRLWVPGVRAELRAHGKAVGLPSDDDMGNSEVGHNALGAGKVYAQGAVLVGRAIDS
                     GALFQGPVWREVVARGAAGGAIHFLGLLSDGNVHSHISHLEALLAAAARAGARRLYVH
                     ALLDGRDVAPTSALEYIDRLERYLADLRGSGADARIASGGGRMQITMDRYEADWKMVE
                     RGWHTHVLADARPFASATAAVETLRAEQPGVIDQDLPPFVVTPPAPVQDGDAVVMFNF
                     RGDRAIEISRAFDDPELRAFDRVRRPDVLYAGMMEYDGDLHIPRHFLVAPPEIDRPMG
                     ELLAAAHIPQLAISETQKYGHVTYFWNGNRSGMFDRGTETYIEIPSDTLPFDQRPWMK
                     AAEITDRLIAELRTGKHRFARVNYANGDMVGHTGSFDATVIAVETVDLQLARLARAIE
                     ELQGILVVTADHGNADEMFQRDKSGQIARDKKTGQPVVKTSHTLNAVPFLIHDPVRSD
                     RYEIDPARARTAGIAHVTATCLELLGLTPPDDLEPSLLRFR"
     gene            3033..3698
                     /locus_tag="VF516_16790"
     CDS             3033..3698
                     /locus_tag="VF516_16790"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8109392.1"
                     /translation="MTTDSDRPDDVRPAAHAVVPPAPGPDAGASAPGASNGSTKALIG
                     VLLAGVAGLVIFLFVNVSRDGRIHPGPTPAHAECTRGQPDCLPEVNYTDTAGVAYKRD
                     QLAGKVVLVNFWATWCHPCQSEIPALSHVYDKYKAKGVVFLGVMTDNVDSQQLLNFQS
                     DYEMTYPVVRANSDLMVSYHYPDALPTTFVYDRFGKQVLSHVGPLRESDLDGLLGQLV
                     AQN"
     gene            complement(3703..4746)
                     /locus_tag="VF516_16795"
     CDS             complement(3703..4746)
                     /locus_tag="VF516_16795"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX8109393.1"
                     /translation="MIEPLHEHEPPRVRLANLPTRGHWLRYGQTDRPRGEADRFRVWV
                     KRDDQTGSELMGNKVRKLEYLMAEAVAEDATHVITCGGEQSNHARATAFAAAQLGLSS
                     ILILRTDDPENPPAATGNILLDRLVGAELQWISRPAWRDRNQLLAEAADRVRRAGDRP
                     YVIPEGGSNALGSWGYLRAAHELADDLAGIAAPDHPVTVVYACGSGGTAAGLILGAKL
                     LRFAERGIRLAAINVCDDRAYFVDVISRICAEAEARWQLGTNVTEADIDIVDGHVGRG
                     YARSRPEELATIRDVCRSDGIVLDPVYTGKAFHGMVTELAADPQRFGSAIAFLHTGGL
                     FGLVAPSDIAEVL"
     gene            4833..5699
                     /locus_tag="VF516_16800"
     CDS             4833..5699
                     /locus_tag="VF516_16800"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8109394.1"
                     /translation="MKRSAAIIARIDRMVRCALGLGLGVVLSLVLSLADGGRAAAKAW
                     PTPAAGDSASGGPEILFTFDDGPNPKTTPLVLDTLARHHIHAVFFLVGEMAANRSPRV
                     AAIVQRILRDGHVIASHTMKHHNLCRAPEAAAAADIDDGKAAVEQAAGVPTAWFRVPF
                     GVRCDRLERMLDERHLTHFHWDLDPQEWKHNDAGRAVRYVTGELGRAYGREVLLMHDI
                     KQATTKALPQILDWIDAENVRREQSHKKPIRIVDAPALAVERLPRGLIAWLGDATAGA
                     RALPAALASVLP"
     gene            5837..>6163
                     /locus_tag="VF516_16805"
     CDS             5837..>6163
                     /locus_tag="VF516_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109395.1"
                     /translation="MGQETTITSISMGAGEAAPGASARPQLVLVAISDLPHEPSSRHL
                     LDDVDEVRFGRGERSAVRTRVDGRRVLELRVPDRRMSADHGRLVRGPSGWVLDDPDSK
                     NGAIVDG"
BASE COUNT          935 a         2260 c         2126 g          842 t
ORIGIN      
        1 gtccccagcc gccccggcct ctcccggcgc ccgcggcccg gcgcacatcg cgccgagcgt
       61 gacgctgctc gcggcaccgg ggatcccggc gaccgccccg gaggtcgtcg accgcgtcca
      121 gcagttctac gccggcatca agcaggtgac cgcacagttc cgccagtcgg tcaccaacaa
      181 cacgttcggc agcaccaaga ccagcgacgg ctcggtctgg atcatgaagc ccggcaagat
      241 gcgctgggac tacctcgaga agaggaagga cgccgtcgag gtcaagaaga gcttcatctc
      301 gaacggctcc tccctctacg tcgtcgagca cgacaacaag caggtcgtga agaagaacct
      361 gcagcaggac ctgatgccgg tcgcggtgtc gttcctgtac ggcaagggcg acctgcgcgc
      421 cgagttcaac gccgagatcg acaagaccgg caagtacggt gagaaggacg acatcgtcct
      481 ccggctcacc cccaagcagg cgtcggcgca gtacaagaac ctctacctgg tggtctcgcc
      541 cagggacttc cacgtctcgc agtcggtgat cgtcgactcg tcgagcaacg tgaaccactt
      601 ccggttcttc gcgcccgact tcgagaagcc gatcaaggac agctacttcg agttcgacga
      661 gcgcagcgtc aagaactacc gcgtgatcga cgccgacgcg tccagggacg gcctcgccgg
      721 caccggcgga ctgactccgc cggccgcgcc gccggccaag ccgccgccgg ccaagccgcc
      781 gaccacgaaa ccgtagcggg ccctagcgat acggcttcga tccgccgtag tcgacgacgt
      841 cgctcatgta ctccgaaccg atcgggatcg actccgggtt ctcgcagaac agctcgacga
      901 agccgcacgt gctgcagatg tagcgcgcga tcaggccgcg cggccgcgtg aggtcgaggc
      961 cccggacctc gggagcgccg atcatgacgc cgacctggtg gcggacgatc gacacgtcgt
     1021 aggtcgccgc catcggcgcg tacatcgcgc cgatcgaacc gaaatcggcg cccagccact
     1081 tgctggcggt gtcgatcacg aagcgctcgc gcggaaacac ccggatgaac cgcgtgcccc
     1141 ggcagcgcgg gcacggcacg tcgggccggc gcggcggcgg catcggggtg gtgccgatct
     1201 tggtggcaca cggcgcgcag atggtctgga tcagctcgga ctcggtctcg agccgcgcgg
     1261 cgtggcacag cttgcagttc gccatggctc attgtggcag gcccctgcag gcccgtggca
     1321 cgcacgcggc cgcacgcggc acgcgactct cgcccccgaa ccgcgcccgt gcgctaaggt
     1381 cccggcatgc cagcgccctc cctccgtccc cacccgcgcc agccgtcgcc ccgggggccg
     1441 gtcgtgctgg tcgtgatgga cggcgtgggc cgtggcgccg gcgacgaggc cgacgcggtc
     1501 gcgatcgccg cgaccccgac cctcgatcgg ctctgggtcc cgggtgtgcg cgccgagctc
     1561 cgggcacacg gcaaggcggt cggcctgccg agcgacgacg acatgggcaa cagcgaggtc
     1621 ggccacaacg ccctcggcgc cggcaaggtc tatgcccagg gcgccgtgct ggtcggccgc
     1681 gcgatcgact ccggcgcgct gttccagggc ccggtctggc gcgaggtggt ggcgcgcggc
     1741 gccgccggcg gcgcgatcca cttcctgggc ctcttgtccg acggcaacgt ccactcgcac
     1801 atctcgcacc tcgaggcgct gctcgccgcc gccgcgcgcg ccggggcccg ccggctgtac
     1861 gtccacgcgc tgctcgacgg ccgcgacgtc gcgccgacca gcgcgctgga atatatcgac
     1921 cgcctggagc ggtacctcgc agacctccgc ggatccggcg ccgatgcccg gatcgcctcc
     1981 ggcggcggcc gcatgcagat cacgatggac cgatacgagg cggactggaa gatggtcgag
     2041 cgcggctggc acacccacgt gctcgccgac gcccggccgt tcgcctcggc gaccgccgcg
     2101 gtcgagaccc tgcgcgccga gcagcccggc gtcatcgacc aggacctgcc gccgttcgtg
     2161 gtcacgcctc cggccccggt ccaggacggc gacgccgtcg tgatgttcaa cttccgcggc
     2221 gaccgcgcga tcgagatctc gcgcgcgttc gacgaccccg agctccgcgc gttcgaccgc
     2281 gtgcgccggc ccgacgtgct ctacgccggc atgatggagt acgacggcga cctccacatc
     2341 ccgcggcact tcctcgtcgc gccgcccgag atcgaccggc cgatgggcga gctcctggcc
     2401 gcggcgcaca tcccccagct cgcgatctcc gagacccaga agtacggcca cgtcacctac
     2461 ttctggaacg gcaaccgcag cgggatgttc gaccgcggca ccgagaccta catcgagatc
     2521 ccgtccgaca ccctgccgtt cgaccagcgg ccgtggatga aggccgccga gatcaccgac
     2581 cggctcatcg ccgagctccg gaccggcaag caccgcttcg cgcgcgtcaa ctacgccaac
     2641 ggcgacatgg tcggccacac cggctcgttt gacgcgaccg tgatcgcggt cgagaccgtc
     2701 gacctccagc tcgcccggct cgcccgcgcc atcgaggagc tccagggaat cctggtggtt
     2761 acggcagatc atggcaacgc ggacgagatg ttccagcgcg acaagtccgg ccagatcgcg
     2821 cgcgacaaga agaccggtca gccggtggta aagaccagcc acacgctcaa cgctgtgcca
     2881 ttcttgatcc acgatccggt ccggtccgac cgctacgaga tcgatcccgc gcgggctcgc
     2941 accgccggca tcgcccacgt gacggccacc tgcctggagc ttctcggcct gaccccgccc
     3001 gacgacctgg agccttccct gctgaggttt cgatgacgac agactccgat cgccccgacg
     3061 acgtccgccc cgctgcacat gcagtggtcc ccccggcccc cgggcccgac gccggcgcct
     3121 ccgcccccgg tgcctcgaac ggcagcacca aggcgctgat cggcgtcctg ctcgccggcg
     3181 tcgccggcct ggtgatcttc ctgttcgtca acgtgtcgcg cgacggccgg atccaccccg
     3241 gcccgacgcc ggcgcacgcc gagtgcaccc gcggccagcc cgactgcctg cccgaggtca
     3301 actacaccga caccgccggc gtcgcctaca agcgcgatca gctcgccggc aaggtcgtgc
     3361 tggtcaactt ctgggcgacc tggtgccatc cctgccagag cgagatcccg gcgctgtccc
     3421 acgtctacga caagtacaag gccaagggcg tcgtgttcct cggcgtgatg accgacaacg
     3481 tcgacagcca gcagctgctc aacttccagt ccgactacga gatgacctac ccggtcgtcc
     3541 gcgccaactc cgacctcatg gtctcctacc actaccccga cgcgctgccg acgaccttcg
     3601 tctatgaccg cttcggcaag caggtgctca gccacgtcgg cccgctgcgc gagagcgacc
     3661 tcgacgggct gctcggccag ctcgtcgcac agaactgacc gatcacagca cctcggcgat
     3721 gtccgaaggt gcgaccaggc cgaacagtcc gccggtgtgg aggaaggcga tcgcgctgcc
     3781 gaaccgctgc gggtcggcgg cgagctcggt gaccatgccg tggaacgcct tgccggtgta
     3841 gaccgggtcg agcacgatgc cgtcgctgcg gcacacgtcg cggatcgtgg cgagctcctc
     3901 ggggcggctc ctggcgtagc cgcggccgac gtggccgtcg acgatgtcga tgtcggcctc
     3961 ggtgacgttg gtgccgagct gccagcgcgc ctcggcctcg gcgcagatcc ggctgatcac
     4021 gtcgacgaag taggcgcggt cgtcgcacac gttgatcgcg gcgagccgga tgccgcgctc
     4081 ggcgaagcgc agcagcttgg cgccgaggat caggccggcg gcggtgccgc ccgagccgca
     4141 ggcgtagacc acggtgaccg ggtgatcggg cgcggcgatg ccggcgaggt cgtcggcgag
     4201 ctcgtgggcg gcgcgcaggt agccccagct gccgagcgcg ttcgagccgc cctcggggat
     4261 gacgtagggc cggtcgccgg cgcgccgcac gcggtccgcg gcctcggcga gcagctggtt
     4321 gcgatcgcgc cacgccggcc gcgagatcca ctgcagctcg gcgccgacca ggcggtcgag
     4381 caggatgttg ccggtcgccg ccggcgggtt ctccggatca tcggtgcgca ggatcaggat
     4441 gctgctcagg ccgagctgcg cggcggcgaa cgcggtcgcc cgcgcgtggt tggactgctc
     4501 gccgccgcac gtgatcacgt gggtcgcgtc ctcggcgacc gcctcggcca tcaggtactc
     4561 cagcttgcgg accttgttgc ccatgagctc gctgccggtc tggtcgtcgc gcttgaccca
     4621 gacccggaag cggtcggcct cgccgcgcgg ccggtcggtc tggccgtagc gcagccagtg
     4681 gccgcgggtc ggcaggttgg cgagccgcac gcggggcggc tcgtgctcgt ggagcggttc
     4741 gatcatgcgg tgaatgtaga cgaccggccg tgccgctgcg ctgcgatcgc ccgaaacatg
     4801 acgcctgcag caggcgtgtt agacccgaag gggtgaaacg cagcgcagcg atcatcgcgc
     4861 ggatcgatcg gatggtccgg tgtgccctgg ggctcggtct cggggtcgtt ctcagcctcg
     4921 tgctctcgct cgcggacggc ggccgcgcgg ccgccaaggc ctggccaacg ccggcggccg
     4981 gtgactcggc gtcgggcggc cccgagatcc tgttcacgtt cgacgacggc cccaacccga
     5041 agaccacacc gctggtgctc gacacgctcg cccggcacca catccacgcg gtgttcttcc
     5101 tggtcggcga gatggcggcg aaccggagcc cccgggtcgc cgcgatcgtc cagcggatcc
     5161 tgcgcgacgg ccacgtgatc gcgagccaca cgatgaagca ccacaacctg tgccgcgctc
     5221 cggaggccgc ggccgccgcc gacatcgacg acggcaaggc cgcggtcgag caggccgccg
     5281 gcgtgccgac cgcgtggttc cgcgtcccgt tcggcgtgcg ctgcgaccgg ctggagcgca
     5341 tgctcgacga gcgccacctc acgcacttcc actgggatct cgatccccag gagtggaagc
     5401 acaacgacgc cgggcgcgcg gtccgctacg tcaccggcga gctcggccgc gcctacggcc
     5461 gcgaggtcct gctgatgcac gacatcaagc aggcgacgac caaggcgctg ccccagatcc
     5521 tcgactggat cgacgccgag aacgtgcggc gcgagcagtc gcacaagaag ccgatccgga
     5581 tcgtcgacgc gccggcgctg gcggtcgagc ggctgcccag gggcctcatc gcctggctcg
     5641 gcgatgccac cgccggtgcc cgcgcgctgc ccgccgcgct cgccagcgtg ctgccgtagc
     5701 cagcccgcgc gtttccgcac ccgggcgtcg ggacggtgcg ggatcgcggc cagggcgacg
     5761 accggaggat cgccagggag tggggccggg gagtggggcc agggaggaag gccaggagta
     5821 aggatagact agtcgcgtgg gccaggagac cacgatcacc agcatctcga tgggcgccgg
     5881 cgaggccgcg cctggcgcga gcgcgcgccc gcagctggtg ctggtcgcga tcagcgatct
     5941 gccgcacgag ccgtcgtcgc gccacctgct ggacgacgtc gacgaggtcc ggttcgggcg
     6001 gggcgagcgg tcggcggtgc ggacccgggt cgacggccgg cgcgtgctgg agctccgggt
     6061 ccccgatcgc cggatgtcgg ccgaccatgg ccggctggtg cgcggaccgt cgggctgggt
     6121 gctcgacgat ccggactcca agaacggcgc gatcgtcgac ggg
//
LOCUS       DASVOR010000856         7351 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_154717,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000856 DASVOR010000000
VERSION     DASVOR010000856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154717"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF516_16810"
     CDS             complement(<1..250)
                     /locus_tag="VF516_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109396.1"
                     /translation="MQTSTLPWRARGAPSLPEVHRTVAVNTRGALVRRVLAFAGPGYL
                     VAVGYMDPGNWATGVAAGSAFGMSLLWVIVLSNLMAITL"
     gene            complement(262..1158)
                     /locus_tag="VF516_16815"
     CDS             complement(262..1158)
                     /locus_tag="VF516_16815"
                     /inference="COORDINATES: protein motif:HMM:NF016925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEX8109397.1"
                     /translation="MFMHHKKLQVTVRVDQTNPSLANLMLEQFGGPQGELAAAMRYFT
                     QSLAELDPGRKDLLLDIATEELSHLEVIGTVVSMLNKGARGKIAEATESHAQMYREIS
                     GSQNDSHITQLLYGAGAPLTNSAGLPWTAAYIDTITDPTADLRSNIAAEARAKIIYER
                     LIGLTDDPGVRDTLCFLMTREIAHQKQFEKALYSIEHNFPPGRLPGEPRFTNVYFDLS
                     QGPGNETGPWNRPDHWKVIENREDQMAVEGGDGQAHVNLTHQEAKAVQAAAERTKSNE
                     NCDPVTGADLGASNQCAPDRQG"
     gene            1723..2445
                     /locus_tag="VF516_16820"
     CDS             1723..2445
                     /locus_tag="VF516_16820"
                     /inference="COORDINATES: protein motif:HMM:NF021576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2306 domain-containing protein"
                     /protein_id="HEX8109398.1"
                     /translation="MRVPSRLLTPLRVLVLIALAAGSAAITASSLAYFDDDLAPFVLE
                     KLPLPLEDVWMLALRMHVVAAAVCLPGCLLLSLGIVIRRPRLHRWLGRVTAAAVLLAL
                     VPSGLYLSWFAKGGLPSTVGFMLSGAIVAVAMVQGVRTAWARRFVEHRRWVLHVLAQL
                     SVAVTSRVMLFAFDAAGVDEQLAYLVSLWLPVAGSFGLVELLSIRTRTRTAPSTRRTH
                     EAPVDARHSPLLPYDLRRAHGG"
     gene            2390..2863
                     /locus_tag="VF516_16825"
     CDS             2390..2863
                     /locus_tag="VF516_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109399.1"
                     /translation="MLAILLFSRTTFAEPTAASGAPPDAKSAEAKTAQALVSRELIQP
                     LAAKESTRSKFSRASLPAQARRVRILDDQPQQDARGNAFVRFAVDAHHGFTADDDESR
                     WQLATITGCAYLDRSQVFVKRGDEYRPAAFLLGKNLKAAAETTCQPAQAQVAHKD"
     gene            3211..3765
                     /locus_tag="VF516_16830"
     CDS             3211..3765
                     /locus_tag="VF516_16830"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX8109400.1"
                     /translation="MKLRIALTSLLALVLVVCAALGHRAAADHREVALRAALRGLNEV
                     PPTTSRATATLRATLDEAGQRISFTLEYRNMMANPTAAHFHFGPTKVNGGVMVFLCGG
                     GGQPACPPATSGKITGTITAANVVGPGDQGIAPRNFADVVYAILTGNAYVNIHSSRFP
                     NGELRGQVEPSGAGRAEDRAGSED"
     gene            complement(3762..6899)
                     /locus_tag="VF516_16835"
     CDS             complement(3762..6899)
                     /locus_tag="VF516_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase domain-containing protein"
                     /protein_id="HEX8109401.1"
                     /translation="MSGPQDRRWIEPWIVGTAASALAVGCQPAGETSQIVSAVTLPVT
                     VTNAMYKIQKTNPDPYHAFSGGVATIGAAKNEFEAFQIVIPGLANNVNATASDLTCSS
                     GGCAGARITAASCTSGTGTGGNVRLYRERYYDTVIGSSNWSDTHAMLGTLPDALIPQV
                     DEFYNECRAFGFSVPSGSNGVIWVDVFVPQNQPAGTYTGSVTVTSDQGSATVPVSLQV
                     WDFALPPTATLHSTFGMSGSAIRVQHAAGAICDPAIASLHRLYGALGLDHRVTINMLD
                     DECNNTASYIASTYGALFDGTLSTAPAITRLAGAHTTSIQYLQGLTDSTALASWASTM
                     HAHTGWFQQLFQYTADEPSGSGFSSIAPAAMAAHAADPNFRTLVTTDLDEAYAAGVNY
                     NRDINRFVEIIDHLEGTSGNERWEYNDYPQGSGANWQAINPINEVWTYQSCDSHGCGA
                     AGTDRWPSYMIDARPMRNRALEWFSFKNRLQGELYYDVNFAWNGGEWTNQFYFSGHGD
                     GTLLYPGTPAAVSGGSTEAIGGSHHIPIASLRLKLIREGMEDYEYMALLDSQGDRAFA
                     ESEIAAVFPGGAVNTEGDPEMLYAKRQEMACRILTHLGKPCPGGPQTLTVTRTGTGSG
                     TVTSTPAGISCGATCSASFSPGTVTLTATASAGSTFTGWSGDCTGTGACSLDMSVSHN
                     ATATFTASGGCSVDDCFDRADGALGASWNVVQPTIQIVSSQAVATGTGAESAVFLDNV
                     GPDQDVSAVCTIAVSSGSNCGVMARYTDADNLYYAYFDIGLQRVDLWRRSGGVVTQLG
                     SAARTLVAGTPYPLRLVVTGSSLQVYFNSESTPAISATDSVLPTGSYGGVHAFANTAS
                     SVRWDNFNVAASGALFYDTFDRTTGLGASWNITAGGGFTTDGTYAVSTTSQNWARIVP
                     SLGTNDYRVTAELIVPSGSLFSGIIARTATINTDETMYAAQLSTDGHVNLYRRNAYTW
                     TLLAANPATITAGVQYTVQLAVSGSNPVHLEVWLNGVQQISFDDASPGRITAGIPGIE
                     NYDVGVKYNSFRVDR"
     gene            complement(7165..>7351)
                     /locus_tag="VF516_16840"
     CDS             complement(7165..>7351)
                     /locus_tag="VF516_16840"
                     /inference="COORDINATES: protein motif:HMM:NF023326.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HEX8109402.1"
                     /translation="LWQGYLRRRDELRARDRSLDELDEFRWLVEELRVQIFAPELKTA
                     VPVSPQRLQEVWARLSR"
BASE COUNT         1061 a         2528 c         2552 g         1210 t
ORIGIN      
        1 gcagcgtgat cgccatcagg ttcgacagca cgatcaccca cagcagcgac atcccgaacg
       61 ccgagcccgc cgcgacgccg gtcgcccagt tccccggatc catgtatccg accgccacga
      121 ggtagcccgg gcccgcaaac gcgagcacgc ggcgcacgag cgccccccgg gtgttcaccg
      181 cgaccgtgcg atggacctcg ggcagactcg gcgctccacg ggcgcgccac ggcagggtgg
      241 aggtctgcat ggcgctggcg gtcagccttg ccggtcggga gcgcactggt tgctggcgcc
      301 gagatccgcg ccggtcaccg gatcgcagtt ctcgttggac ttggtgcgct cggcggcggc
      361 ctggaccgcc ttggcctcct ggtgcgtcag gttgacgtgg gcctgaccgt cgccgccctc
      421 gaccgccatc tggtcctcgc ggttctcgat gaccttccag tggtcggggc ggttccacgg
      481 cccggtctcg ttgcccgggc cctgcgacag gtcgaagtac acgttggtga accgcggctc
      541 gcccggcagc cggcccggcg gaaagttgtg ttcgatcgag tacagtgcct tctcgaactg
      601 cttctggtgc gcgatctcgc gggtcatcag gaagcacagg gtgtcgcgca cgcccggatc
      661 gtcggtcagg ccgatcagcc gctcgtagat gatcttcgcg cgcgcctcgg cggcgatgtt
      721 cgagcgcaga tccgcggtcg gatcggtgat cgtatcgatg tacgccgcgg tccacggcaa
      781 accggcggag ttggtcagcg gggccccggc gccgtacagc aactgggtga tgtggctgtc
      841 gttctggctg cccgagatct cccggtacat ctgggcatgc gactcggtgg cctccgcgat
      901 cttgcccctg gcacccttgt tcagcatcga gaccaccgtg ccgatcacct cgaggtggct
      961 gagctcctcg gtcgcgatgt cgagcagcag gtccttgcgg cccggatcga gctccgcgag
     1021 cgactgggtg aagtagcgca tcgccgcggc gagctcgccc tgcggcccgc cgaactgctc
     1081 gagcatgagg ttggccagac tcggattggt ctggtcgaca cgcacggtga cctgtagctt
     1141 cttgtggtgc atgaacatgg tgacgccact ccttccggcg tgctcgccat gcaacgcatg
     1201 ctccgcagcg cggtcagcgg aacccggcga aaccagacgg tcgcatcgcc gcgagcagcg
     1261 cgccgacgac acgcaggtgc gtcggcgcac cacggacaag cgcgcgtcca ctcgtcatga
     1321 tgaatcacgg agggtattcc acgaatgttg gtcttcatgc gacagccccg caccggcgtc
     1381 cggtccggag tctgccgctc gtgccggcga tcgtgcggcg gggctccgga ccgcgcggtt
     1441 ccaccacgac cgcaccgggt gggagctggt ggtagggtcc ggccatgcgt tacaacctac
     1501 tagggcaaat gcgtcggtcg cgcgcgtcgc gctcgcatgg cagcgcggcg ccgcgcgcac
     1561 gccgaccgag gcggagatcc gcgcggtcgc cgaccgggtg aagtgacgtt cgaacgccga
     1621 tctgaaacaa cgcgcaagac cgcgcgatca ttccgctctg gcggcgctgg ggggccgggc
     1681 cgcgatagac cgcgggctcg cctcgcgcgt tggagaagtt ccatgcgggt tccctcgcgg
     1741 ctgctcacgc ccctgcgggt gctggtcctg atcgcgctgg cggcgggctc ggccgcgatc
     1801 acggcctcca gcctggcgta cttcgacgac gacctggcgc cgttcgtcct cgagaagctc
     1861 ccgctgccgc tcgaggatgt gtggatgctg gcgctgcgga tgcacgtggt cgccgcggcc
     1921 gtgtgcctcc ccggctgtct cctgctgtcg ctgggaatcg tgatccggcg cccgcggttg
     1981 caccgctggc tgggccgcgt caccgcggcg gcggtcctgc tcgcgctggt cccgtccggc
     2041 ctgtacctgt cgtggttcgc caagggcggc ctgccctcga cggtggggtt catgctgtcc
     2101 ggggcgatcg tcgccgtcgc catggtccag ggcgtgcgca ccgcgtgggc gcggcgtttc
     2161 gtcgagcacc ggcgctgggt gctccacgtg ctcgcgcagc tcagcgtggc cgtgacctcg
     2221 cgcgtgatgc tgttcgcgtt cgacgccgcc ggcgtcgacg agcagctcgc ctacctcgtc
     2281 agcctgtggc tgccggtcgc cggaagcttc gggctggtcg agctcctgtc gatccgaacc
     2341 cgtacccgga ccgccccgtc tacccggagg actcatgaag cgcctgtcga tgctcgccat
     2401 tctcctcttc tcccgtacga ccttcgccga gcccacggcg gctagcggcg cgccgcccga
     2461 cgcgaagagc gccgaggcga agaccgccca ggcgctggtc agccgcgagc tgatccagcc
     2521 gctcgccgcc aaggagagca cccggagcaa gttctcccgc gcgagcctgc ccgcgcaggc
     2581 gcggcgagtc cgcatcctcg acgaccagcc gcaacaggat gcgcggggga acgcgttcgt
     2641 gcgcttcgcg gtcgatgccc accacggctt caccgcggac gacgacgagt cgcggtggca
     2701 gctcgccacc atcaccggct gcgcatacct cgatcgcagc caggtgttcg tcaagagggg
     2761 cgacgagtac cggccggccg cgttcctgct gggcaagaac ctgaaggccg ccgccgagac
     2821 gacctgccag ccggcgcagg cgcaggtcgc gcacaaggac tgagcgctgg cttcctcccc
     2881 ggcgagcgcc ggcgcacgcc cgcagcgcag ggggaaaccg cgcggctcga cgacgaccgc
     2941 gcgacgtggc gcgatgcact gggtcgcggc aggtggtcgt tcggatccgg ataccggata
     3001 cgcgacattg ccagcgtcct gcacgatatt gccagcgttg cccgaaaatc actcggtgat
     3061 ggagtgacgg catcttgcgg agtgatggct gtccggctat gagccaatca ccgaaacgcg
     3121 agatgcgcga ggcgtaggac gcaatcgaat cgatgctcgg atgtgggcgt cggcatcatg
     3181 gcatctcgcc acagccacag gggaagggat atgaagctcc gtatcgcact gacatcattg
     3241 ctcgcgctgg tcctcgtggt ctgcgccgcg ctgggccacc gcgccgccgc cgaccatcgc
     3301 gaggtcgcgc tgcgcgccgc gctgcgcggg ctcaacgagg tgccgccgac cacgagccgc
     3361 gcgaccgcaa cgctgcgcgc cacgctcgac gaggccggcc agcggatctc gttcacgctg
     3421 gagtaccgca acatgatggc caacccgacg gcggcgcatt tccacttcgg cccgaccaag
     3481 gtcaacggtg gcgtcatggt gttcttgtgc ggcggcggcg gccagccggc atgtccgccc
     3541 gcgacctcgg gcaagatcac cgggaccatc accgcggcga acgtcgtcgg tcccggcgat
     3601 caggggatcg cgccccgcaa cttcgcggac gtcgtgtacg cgatcctgac cggcaacgcg
     3661 tacgtcaaca tccacagcag caggttcccg aacggcgagc tccgcggcca ggtggagccg
     3721 tccggcgccg gccgcgcgga ggaccgcgcc ggttccgagg actagcgatc gacccggaac
     3781 gagttgtact tcacgccgac gtcgtagttc tcgatccccg ggatgccggc tgtgatccgg
     3841 cccggcgatg cgtcatcgaa gctgatctgc tgcacgccgt tcagccatac ctcgaggtgc
     3901 acggggttcg atccgctcac cgcgagctgt accgtgtact ggacacccgc ggtgatcgtc
     3961 gcggggttcg cggcgagcag cgtccaggtg tacgcattgc ggcggtacag gttgacgtgg
     4021 ccatcggtcg atagctgggc ggcgtacatc gtctcgtcgg tgttgatcgt cgcggtgcgc
     4081 gcgatgatcc ccgagaacag cgagcccgag gggacgatca gctccgcggt cacccggtag
     4141 tcgttggtgc ccaggctggg cacgatgcgc gcccagttct gcgatgtggt ggagaccgcg
     4201 tacgttccgt cggtggtgaa cccgccgcct gccgtgatgt tccagctcgc gcccaggccg
     4261 gtcgtgcgat cgaacgtgtc gtagaacagc gcgccgctcg cggcgacgtt gaagttgtcc
     4321 caccggaccg agctcgcggt gttcgcgaac gcgtgaacac cgccgtagct gccggtcggg
     4381 agcacgctgt cggtcgccga gatcgccggg gtcgactcgc tgttgaagta gacctgcagc
     4441 gagctgccgg tgaccacgag gcgcagcggg tagggcgtcc cggcgaccag cgtgcgcgcc
     4501 gcgctgccga gctgggtgac cacgccgccg ctgcgccgcc acagatcgac gcgctgcagc
     4561 ccgatgtcga agtacgcgta gtacaggttg tcggcgtcgg tgtagcgcgc catgacgccg
     4621 cagttgctgc ccgagctcac ggcgatcgtg cacaccgccg acacgtcctg gtcgggcccg
     4681 acgttgtcca ggaacaccgc gctctccgcc cccgtaccgg tcgccacggc ctgcgacgag
     4741 acgatctgga tcgtcggctg gaccacgttc cagctcgcgc cgagcgcgcc gtctgcgcga
     4801 tcgaagcagt cgtcgaccga acacccgccg gaggcggtga acgtggccgt ggcgttgtgg
     4861 ctcaccgaca tgtcgaggct gcacgcgccg gtgccggtgc agtcccccga ccagccggtg
     4921 aacgtcgacc cggcgcttgc ggtggcggtg agcgtgacgg tacccggcga gaagctcgcg
     4981 ctgcacgtcg cgccgcacga gatcccggcc ggcgtcgacg tgacggtgcc gctcccggtc
     5041 cccgtcctgg tcaccgtcag cgtctgcggc ccgccggggc acggcttgcc gaggtgggtc
     5101 aggatccggc aggccatctc ctggcgcttg gcgtacagca tctccggatc gccctcggtg
     5161 ttgaccgcgc cgccggggaa caccgccgcg atctcggact ccgcgaatgc acgatcgccc
     5221 tggctgtcca gcagcgccat gtactcgtag tcctccatgc cctcgcggat cagcttgagc
     5281 cgcagcgacg cgatcgggat gtggtggctg ccgccgatcg cctcggtgct gccgcccgac
     5341 actgccgccg gcgtccccgg gtagagcagc gtcccgtcgc cgtggccgga gaagtagaac
     5401 tggttcgtcc actcgccgcc gttccaggcg aagttgacgt cgtagtacag ctcgccctgg
     5461 agccggttct tgaagctgaa ccactcgagc gcgcggttgc gcatcggccg cgcgtcgatc
     5521 atgtacgacg gccagcgatc ggtcccggcg gcaccgcacc cgtgcgaatc gcatgactgg
     5581 taggtccaaa cctcgttgat cgggttgatc gcctgccagt tcgcgccgct gccctgagga
     5641 tagtcgttgt actcccagcg ctcgttgccc gacgtcccct cgaggtggtc gatgatctcg
     5701 acgaaccggt tgatgtcgcg gttgtagttg acgccggcgg cgtacgcctc gtcgagatcg
     5761 gtcgtgacca gcgtgcggaa gttcggatcc gccgcgtggg cggccatcgc ggccggagcg
     5821 atgctggaga accccgagcc gctcggctcg tccgcggtgt actggaacag ctgctggaac
     5881 catccggtgt gggcgtgcat cgtcgacgcc cagctcgcga gcgccgtgct gtcggtcagc
     5941 ccttgcaggt actggatcga cgtcgtgtgg gcgccggcca gccgggtgat cgcgggtgcc
     6001 gtggacagcg tgccgtcgaa cagcgcgccg taggtactcg cgatgtagct cgcggtgttg
     6061 ttgcactcgt catcgagcat gttgatcgtc acccggtgat cgagccccag cgcgccgtac
     6121 agccggtgca ggctcgcgat cgccgggtcg cagatcgcgc cggccgcgtg ctggacccgg
     6181 atcgcgctgc cgctcatccc gaacgtcgag tgcagggtgg cggtcggcgg caacgcgaag
     6241 tcccagacct gcagcgatac cggcaccgtc gcgctgccct ggtcggacgt gaccgtgacg
     6301 ctgccggtat acgtgccggc cggctgattc tgcgggacga acacgtcgac ccagatcacg
     6361 ccgttgctgc ccgagggaac gctgaagccg aacgcgcggc actcattgta gaactcgtcg
     6421 acctgcggga tcagcgcatc gggcagggtg ccgagcatgg catgtgtatc cgaccagttc
     6481 gacgagccga tcaccgtatc gtagtagcgc tcgcgataca gccggacgtt gccgccggtg
     6541 ccggtgccgc tggtgcagct cgccgcggtg atccgcgcac ccgcgcagcc gcccgacgag
     6601 caggtcaggt cgctcgcggt cgcattgacg ttgttggcga gcccgggaat cacgatctgg
     6661 aacgcctcga actcgttctt ggcggcgccg atcgtggcga cgccgcccga aaatgcatga
     6721 taaggatcag gattagtttt ctggatcttg tacattgcat tcgtgaccgt cacggggagc
     6781 gtgacggcac tgacgatctg cgaggtctca cccgcgggct gacagcccac ggcgagcgcg
     6841 ctcgccgcgg tccccacgat ccatggctcg atccagcggc ggtcctgcgg tcccgacatc
     6901 gacgacctct tcgagttgtg tttgggattc cgaattgcac tcgctgtgcc ggtgaccggg
     6961 cgctcgcggc gccggctgcg cgcgctcggg ggcgaagcac cgacgccgtc gtcgcctcag
     7021 cgtggcgtgc gagcgcgcgg cgatggtgtg cggcgtcgtg cggcaagacg ggtctcgcaa
     7081 tgtcgcgcgg cgtctcgccc tgccgcgatc ccggtaacgc gaggtgtcat gcggatgagg
     7141 tcggcgccac cacgccgcgg tgcttcagcg cgacagccgc gcccacactt cctgcagccg
     7201 ctgcggtgac accggtacgg cggtcttgag ctcgggcgcg aagatctgga cccggagctc
     7261 ctcgaccagc cagcggaact cgtcgagctc gtcgagcgat cggtccctgg cccgcagctc
     7321 gtcgcgccgg cgcaggtagc cttgccacag c
//
LOCUS       DASVOR010000857         2946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_329948,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000857 DASVOR010000000
VERSION     DASVOR010000857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2946
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329948"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF516_16845"
     CDS             <1..206
                     /locus_tag="VF516_16845"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8109403.1"
                     /translation="TGLARRDAMALGILMNTRGLMELIVLNVGLELGVVSPMLFTLLV
                     IMVVVTTIATTPVLDRLRRGAAI"
     gene            453..>2946
                     /locus_tag="VF516_16850"
     CDS             453..>2946
                     /locus_tag="VF516_16850"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8109404.1"
                     /translation="MEIRPKWLHVAVGALAAGGLITALSSGTPAATSSASFTMHILAP
                     VGPESPEELRMFPTAVKTDSRGRVITGQTSDPVKNIVGAEAAGTRAGVARFTKITFGA
                     QYDTGRPNDSHRVDPNHPALSSGSEHWPHRSQPFRSWPSAVALTPDGSKLYVSLPGRE
                     GYPDFRVAVVNTSSRTVSRWIDLRPSGQTLGTRPIGLRVSPVNTAISAHPYVVVVNEY
                     GNFASVIDTATDTVLGQFETDFYAEDLVFNAAGTRLYTTDRFKNQVHAFRIDAGPTFT
                     ALGTIPTGTSDLDRSHPRDLDISADGNTLYVANTVGHTIAVINIASDANTLVRTMPVG
                     GLATDVKIAGRWGIVSGHDTSNVMNGPETGHGLPTVQNGVAIRNNGQPLGYQPLMTDT
                     TKATTFDDLGSTLSVFDTATNQFVYRYVDTGRDQSMLVTPGQTVDLGDHEAASKIIQG
                     SGPEQLWVRGNFLFVAQMHSDKVEVFSVNQNPSAPSGILAERGFEFTGGITPQGITVS
                     PDGQTVYVANMQTEDVSFLHADATGALTRQGFVTVGVTGSTPDPSRPGNNGSNLFATD
                     EEVGLRWFFTQSYSDDGQKSCGHCHWQSRHDGSQWNVGANAIGGVKSVPQNKDLSDNW
                     PEWFEGLNNNMSAYASACNGELVNAEKLTTLFPQSTLSARLAAREQFVQNATSANSTA
                     IGRSELSGDAQSIGFYKMAFDQILWTQNETRRLPNPRAQFPTSSEASQIARGKQLFST
                     EVGSGGAGCSSCHHNGDQTVNGVPNDTFQDYNLHEPGVISETTVDGDGPFQRLIADYF
                     FIQGGQPQDEGSRQNISSRNTRHLRSFWDGVPR"
BASE COUNT          514 a         1072 c          941 g          419 t
ORIGIN      
        1 cgaccggcct cgcgcgccgc gacgcgatgg cgctcggcat cctgatgaac acgcgcggcc
       61 tgatggagct gatcgtgctg aacgtgggcc tcgagctcgg ggtggtgtcc ccgatgctgt
      121 tcacgctgct ggtgatcatg gtggtggtca ccacgatcgc gaccactccc gtgctcgacc
      181 ggctgcgccg cggcgcagcg atctgacgcc gccgcactcc gcggtcgatt cgcgtattcg
      241 ccgtcacatt gtgttctgcc cgcaatgggg tgcaaatcgt gcgctgccga gatcggcagt
      301 gcggcgctcg tgtagctgta acgttttccc cgccagcgca ctccgttgct ccacgcgcga
      361 tctggaggac gcgacgtgtc caatttgggc gtcctccgcg tcgagatcga cgagatcgcc
      421 gggatcgtct gcaacgacaa ggagagcaga tcatggagat aagaccgaag tggcttcacg
      481 tcgcggtggg ggcgctcgcg gctggagggc tcatcacggc gctgtcatcg ggtacgcccg
      541 cggcgacatc gtccgcgagc ttcacgatgc acatcctcgc cccggtcggt ccggaatcgc
      601 cggaggagct gcggatgttc cccaccgcgg tcaagaccga ctcgcgcggc cgggtgatca
      661 ccgggcagac cagcgatccg gtgaagaaca tcgtcggtgc cgaggccgcc ggcacgcgcg
      721 ccggggtggc gcggttcacc aagatcacgt tcggcgcgca gtacgacacc ggccggccca
      781 acgactcgca ccgcgtcgac cccaaccatc cggcgctgag ctcgggcagc gagcactggc
      841 cgcaccgcag ccagccgttc cgcagctggc cgagcgccgt ggcgctgacc cccgacggca
      901 gcaagctcta cgtctcgctg cccgggcgcg aggggtaccc cgacttccgg gtcgccgtgg
      961 tcaacacctc gagccgcacg gtgagccggt ggatcgacct gcggccgtcc ggacagacgc
     1021 tcggcacgcg cccgatcggc ctgcgggtgt cgccggtcaa caccgcgatc tcggcccacc
     1081 cctacgtggt cgtcgtcaac gagtacggga acttcgcgtc cgtgatcgac accgccaccg
     1141 acaccgtgct cggccagttc gagaccgact tctacgccga ggacctggtg ttcaacgccg
     1201 cgggcacccg gctgtacacc acggatcggt tcaagaacca ggttcacgcg ttccgcatcg
     1261 acgccggccc gacgttcacc gcgctcggca cgatcccgac cggcaccagc gatctcgatc
     1321 gctcgcaccc gcgcgatctc gatatcagcg ccgacgggaa caccctctac gtcgcaaaca
     1381 ccgtcggcca caccatcgcc gtcatcaaca ttgccagtga tgccaatacc ctggtccgca
     1441 ccatgccggt cggcgggctc gccaccgacg tcaagatcgc cggccgctgg ggcatcgtgt
     1501 cgggccacga caccagcaat gtgatgaacg ggccggagac cggccacggc ctgccgacgg
     1561 tgcagaacgg cgtcgcgatc cgcaacaacg gccagccgct cggctaccag ccgctgatga
     1621 ccgacacgac caaggccacg acgttcgacg acctcggcag cacgctcagc gtgttcgaca
     1681 ccgcgaccaa ccagttcgtc taccgctacg tcgacaccgg gcgcgaccag tcgatgctgg
     1741 tgaccccggg ccagaccgtc gacctcggcg atcacgaggc ggcgtcgaag atcatccagg
     1801 gctcgggccc ggagcagctg tgggtgcgcg gcaacttcct gttcgtcgcc cagatgcact
     1861 ccgacaaggt ggaagtgttc agcgtcaacc agaacccgtc ggcgccgtcg gggatcctcg
     1921 ccgagcgcgg cttcgagttc accggcggca tcaccccgca gggcatcacg gtgtcgcccg
     1981 acggccagac cgtgtacgtc gccaacatgc agaccgagga cgtgtcgttc ctccacgccg
     2041 acgccaccgg cgcactgacc cggcagggct tcgtcaccgt cggcgtcacc ggcagcaccc
     2101 cggacccgag ccggcccggc aacaacggct ccaacctgtt cgccaccgac gaggaggtcg
     2161 gcctgcgctg gttcttcacc cagtcgtact ccgacgacgg ccagaagtcg tgcggccact
     2221 gccactggca gagccgccac gacggcagcc agtggaacgt cggcgccaac gcgatcggcg
     2281 gcgtcaagtc ggtgccgcag aacaaggacc tgtccgacaa ctggcccgag tggttcgagg
     2341 gcctcaacaa caacatgtcg gcctacgcgt cggcgtgcaa cggcgagctg gtcaacgccg
     2401 agaagctgac cacgctgttc ccgcagtcga cgctgtcggc gcggctcgcc gcgcgcgagc
     2461 agttcgtcca gaacgcgacc tcggccaact cgacggcgat cggacgctcc gagctcagcg
     2521 gcgacgcgca gtcgatcggc ttctacaaga tggcgttcga ccagatcctg tggacgcaga
     2581 acgagacccg ccggctgccc aacccgcgcg cgcagttccc gacctcgagc gaggccagcc
     2641 agatcgcccg cggcaagcag ctgttcagca ccgaggtcgg atcgggcggc gccggctgct
     2701 cgagctgcca ccacaacggc gaccagacgg tcaacggcgt gcccaacgac acgttccagg
     2761 actacaacct ccacgagccc ggcgtgatca gcgagaccac ggtcgacggc gacgggccgt
     2821 tccagcggct gatcgccgat tacttcttca tccagggcgg ccagccccag gacgagggca
     2881 gccggcagaa catctcgagc cgcaacaccc gccacctgcg cagcttctgg gacggcgtgc
     2941 cgcgct
//
LOCUS       DASVOR010000858         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_446676,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000858 DASVOR010000000
VERSION     DASVOR010000858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_446676"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VF516_16855"
     CDS             <1..262
                     /locus_tag="VF516_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8109405.1"
                     /translation="VTLDGNPIDVTAYEFSILRALAQRPGRVLSREQLLDLAKGSAEL
                     SFDRSIDVHVSRLRAKLGDDSRNPKILKTVRGAGYLLAGGDV"
     gene            259..1593
                     /locus_tag="VF516_16860"
     CDS             259..1593
                     /locus_tag="VF516_16860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8109406.1"
                     /translation="MSAAPAGKPPRRRRLVYRVYGFVAVLAIAIMAALIILPRYTRTP
                     KVLAPHAAVTQYLIDRWSLRSPAGLNDAVFRFYARVLGKLSLYDASGRLLHSNADPPL
                     DAPTEAEKRLLRNEKWLLDFDRIVLRSDDGSILGVYAPDRGGFPWSYVIPLGAAILVL
                     VGLASMWFSRRLVRPLDQLATAARRFGAGDTTARAHLTRADEVGDVGRAFDDMADRTA
                     AVLQAQRQLMGDVSHELRTPLARIRVALELALEDPVAASDVLADVGADLDEIDQLIND
                     IQTTSRLDGEHVKLDREATSVSELVDRATRRFTARHPGRQLQRPPDDAPAGDRPIRCD
                     PVLLRRALDNLLDNAAKYSDAGTPVTLAVQPNGTTIAFEIVDRGIGMSPAELDRAFTP
                     FWRADTSRARKTGGVGLGLALARRIARAHGGDVTLASHPGQGTTARLEVPLA"
     gene            1705..>2015
                     /locus_tag="VF516_16865"
     CDS             1705..>2015
                     /locus_tag="VF516_16865"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEX8109407.1"
                     /translation="MKLKRWLPRIIVFALAVGAAAFLLVKKPWAKGDDPITFSTVTVS
                     KGSIAAQVTANGTLSAVKTVQVGAQVSGRVVELHADFNDKVKQGQVIAKLDDVVLKSQ
                     I"
BASE COUNT          291 a          751 c          676 g          297 t
ORIGIN      
        1 ggtgacgctc gacggcaacc cgatcgacgt caccgcctac gagttctcga tcctgcgcgc
       61 gctggcccag cgccccggcc gcgtgctgtc gcgcgagcag ctgctcgatc tcgccaaggg
      121 ctcggccgag ctcagcttcg atcgctcgat cgacgtccac gtgtcgcggc tgcgcgccaa
      181 gctcggcgac gacagccgca acccgaagat cctgaagacc gtgcgcggcg cgggctacct
      241 gctcgccgga ggcgatgtgt gagcgctgcg cccgccggca agccgccgcg gcggcgccgc
      301 ctggtctacc gggtctacgg gttcgtcgcc gtgctcgcga tcgcgatcat ggcggcgctg
      361 atcatcctgc cgcgctacac ccgcacgcca aaggtcctcg cgccccacgc cgcggtgacc
      421 cagtacctga tcgatcgctg gtcgctgcgc agtccggccg gcctcaacga cgctgtgttt
      481 cggttctacg ccagggttct cggcaagctg tcgctgtacg acgccagcgg ccgcctcttg
      541 catagcaacg cggatccgcc gctcgatgcg ccgaccgagg ccgagaagcg cctgctgcgc
      601 aacgagaagt ggttgctcga tttcgatcgg atcgtgctgc gcagcgacga cggttcgatc
      661 ctcggcgtgt acgcgccgga tcgcgggggc ttcccgtgga gctacgtgat cccgctcggc
      721 gcggcgatcc tcgtgctggt cgggctcgcg tcgatgtggt tctcgcgccg gctggtgcgc
      781 ccgctcgacc agctcgccac cgcggcgcgc cggttcggcg ccggcgacac caccgcgcgc
      841 gcccacctga cccgcgccga cgaggtcggc gacgtcggcc gcgcgttcga cgacatggcc
      901 gaccgcaccg cggcggtgct gcaggcgcag cgccagctga tgggggacgt gtcacacgag
      961 ctgcgcaccc cgctcgcccg gatccgggtc gcgctggagc tcgccctgga ggatccggtc
     1021 gcggcgtccg acgtgctcgc cgacgtcggc gccgacctcg acgagatcga tcagctgatc
     1081 aacgacatcc agaccacgtc gcggctcgac ggcgagcacg tcaagctcga ccgcgaggcg
     1141 acctcggtca gcgagctggt cgatcgcgcg acccggcggt tcaccgcccg ccaccccggc
     1201 cgccagctgc agcgccctcc cgacgatgca ccggccggtg accggccgat ccgctgcgat
     1261 ccggtgctgc tgcgccgggc gctcgacaac ctgctcgaca acgccgccaa gtactcggac
     1321 gcgggtaccc cggtcacgct cgcggtccag cccaacggca ccacgatcgc gttcgagatc
     1381 gtcgaccgcg ggatcgggat gtcgccggcc gagctcgacc gcgcgttcac gccgttctgg
     1441 cgcgccgaca ccagccgggc ccggaagacc ggcggggtcg gcctcggcct ggcgctcgcg
     1501 cgccggatcg cgcgcgcgca cggtggggat gtcacactcg cgtcgcaccc gggacagggc
     1561 acgacggcgc ggctcgaagt cccgctcgcg tgacggccat gccgcgctgc tcccgccgcc
     1621 gtccgcatgg acggaatctt caccgcaaca taccgcaata atcgagcaac tttcggttcg
     1681 taccgaggta tcaccttgcc caccgtgaag ctaaaacgct ggctcccccg gatcatcgtg
     1741 ttcgcgctcg cggtcggcgc ggccgcgttc ctcctcgtca agaagccgtg ggcgaagggc
     1801 gatgatccga tcacgttctc gacggtgacg gtgtccaagg gcagcatcgc ggcccaggtc
     1861 accgccaacg gcacgctgtc ggcggtcaag accgtccagg tcggcgccca ggtgtcgggc
     1921 cgcgtcgtcg agcttcacgc cgacttcaac gacaaggtca agcagggcca ggtcatcgcc
     1981 aagctcgacg acgtcgtgct gaagtcgcag atcga
//
LOCUS       DASVOR010000859         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_534666,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000859 DASVOR010000000
VERSION     DASVOR010000859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534666"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(476..1540)
                     /locus_tag="VF516_16870"
     CDS             complement(476..1540)
                     /locus_tag="VF516_16870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8109408.1"
                     /translation="MTTRPTTEAERITELEDELAAARRTIDVLITRLERTGTAPPAQG
                     ELFDAAVRLGNVLEERTREVEDARAEFRALRANLDQIVRQRTRALAESEAQLRARNAE
                     LVRQGKIKAEFISIAAHELRTPLTSIVGYLDLFQEGRFGELPPLIGRPLSSVRRNAHR
                     LKRLVDDMLDVSRIEQGRMPLRREPVNLGEVVSDVVTELEPMAKARELHVATHIREVE
                     SIDADRDKLHQIAVNLVTNAIRYTPEQGEILIDVDEAPSERYPGGWARLRVRDNGVGI
                     AEEDRQRIFDPFLHIHPAKHHTSALPDSAGLGLFIAKGLVDLHGGLITVDSQPDVFTE
                     FTVLLPFKSHQRRDSEPPER"
     gene            complement(1537..>2383)
                     /locus_tag="VF516_16875"
     CDS             complement(1537..>2383)
                     /locus_tag="VF516_16875"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX8109409.1"
                     /translation="LTHSHDAVHRAAAALGTSADALEPGRHVAITLVDGRYDREEAFC
                     IGTAAAAPRIRFVGGCTSTDLDHGDGASPRTSQTSVWVNGEVLTDAGVVVVLESDQPF
                     HAVTSAHLVPTELKTVVTAVSGRVIEQLDGRPAAPRLRQLIASIGDRLDEPRPAHAFA
                     RYLDGVPYVRSLHEIAGDHLVLTCAVEPGHVLRVMQPGDLLGATRRDLATATTRLGGS
                     IAAFLAFSSTGRHDEAVARGTDRELAAAYAACHAVGFHTASEQSGMLLVNHTLTGLAI
                     GAPKS"
BASE COUNT          338 a          811 c          871 g          363 t
ORIGIN      
        1 cgcctcgctg ccgtcgagct caacgctcga gtcaaggtcg acgtcatcgt cgacgttctt
       61 cgtgtcgacg tcgagcgtaa cgggaggagc cacgagcgcg caacttgtga cgaatcatca
      121 gacgtcgccg tcgacctcaa cgctctgggt caacgatcag gtcgacgtcg acgatctcga
      181 acgccatatc gaagacttgt gacgaatcat cagacggcga cgatgacgtc tgatcccacc
      241 cttgaccttg cacctggact gcggtgggaa acagccgcgc gccggcggag ccgctacgtc
      301 acgatcgcgg cgatgtggac ggagatgagc tgaggtcgac gccgaggtcg acggtggggg
      361 caacgtcgta cgtcgccgtc aacctcgacg ttcggtgtca acgtcgacgt caaaccgtcg
      421 acgtcagcaa ccgccaggcc gcgtcgctcc ggcgacagaa gatggctcgc gcgagctaac
      481 gctcgggcgg ctcggagtcc cgccgctggt ggctcttgaa cggcaagagc accgtgaact
      541 cggtgaacac gtcgggctgc gagtccaccg tgatcagccc gccgtggagg tcgaccaggc
      601 ccttggcgat gaacagcccg agccccgccg agtcgggcag cgccgaggtg tggtgcttgg
      661 cggggtggat gtggaggaac ggatcgaaga tccgctggcg gtcctcctcg gcgatcccga
      721 ccccgttgtc gcgcacgcgc agccgcgccc agccgccggg gtagcgctcg ctgggcgcct
      781 cgtcgacgtc gatcaggatc tcgccctgct ccggggtgta gcggatcgcg ttggtcacca
      841 ggttgaccgc gatctggtgc agcttgtcgc ggtcggcgtc gatcgactcg acctcgcgga
      901 tgtgggtcgc gacgtggagc tcgcgggcct tggccatcgg ctcgagctcg gtcacgacgt
      961 cgctgacgac ctcgccgagg ttgaccggct cgcggcgcag cggcatccgg ccctgctcga
     1021 tccgcgacac gtcgagcatg tcgtcgacca gccgcttgag gcggtgcgcg ttgcgccgca
     1081 ccgagctcag cgggcggccg atcagcggcg gcagctcgcc gaaccggccc tcctggaaca
     1141 gatcgaggta gccgacgatg ctggtcagcg gcgtgcgcag ctcgtgggcg gcgatcgaga
     1201 tgaactcggc cttgatcttg ccctggcgca ccagctcggc gttccgcgcg cgcagctggg
     1261 cctcggactc ggcgagcgcg cgggtccgtt gccgcacgat ctggtcgagg ttggcgcgca
     1321 gcgcgcggaa ctcggcgcgg gcgtcctcga cctcgcgggt gcgctcctcc agcacgttgc
     1381 cgagccggac cgcggcgtcg aacagctcgc cctgggcggg cggcgcggtg ccggtgcgct
     1441 ccagccgggt gatcaggaca tcgatcgtcc gccgcgcagc ggccagctcg tcctccagct
     1501 cggtgattcg ttccgcttcc gtcgtgggac gcgtggtcat gatttcggtg ctccgatcgc
     1561 gagaccggtg agggtgtgat tgacgagtag catcccggac tgctcgctcg cggtatggaa
     1621 gcccacggcg tggcacgccg cgtaggcggc ggcgagctcg cggtcggtcc cgcgcgccac
     1681 ggcctcgtcg tggcgccccg tgctggagaa ggccaggaat gccgcgatgc tgccgccgag
     1741 ccgggtcgtc gcggtcgcga ggtcgcgccg ggtggcgccg agcagatcgc cgggctgcat
     1801 cacgcgcagc acgtggccgg gctcgaccgc gcaggtcagc accaggtgat cgccggcgat
     1861 ctcgtgcagc gatcgcacgt agggcacgcc gtcgaggtag cgcgcgaacg cgtgcgccgg
     1921 ccggggctcg tcgagccggt cgccgatgct cgcgatcagc tggcgcagtc gcggcgccgc
     1981 cggccggccg tcgagctgct cgatcacccg gcccgacacc gcggtgacca cggtcttgag
     2041 ctcggtcggg accaggtgcg ccgaggtcac cgcgtggaac ggctgatcgc tctccagcac
     2101 gaccaccacg ccggcgtcgg tcaggacctc gccgttgacc cacaccgagg tctgcgacgt
     2161 gcgcggcgac gcgccgtcgc catgatcgag atcggtcgag gtgcagccgc cgacgaaccg
     2221 gatccgcggc gcggccgcgg ccgtgccgat gcagaacgcc tcctcgcgat cgtagcggcc
     2281 gtcgaccagc gtgatcgcga cgtggcggcc gggctccagc gcgtcggccg aggtgccgag
     2341 cgccgccgcg gcgcggtgga ccgcgtcgtg gctgtgggtg agc
//
LOCUS       DASVOR010000860         4166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_37777,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000860 DASVOR010000000
VERSION     DASVOR010000860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4166
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37777"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(410..1396)
                     /locus_tag="VF516_16880"
     CDS             complement(410..1396)
                     /locus_tag="VF516_16880"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8109410.1"
                     /translation="MKLLGTTGVKISRVALGTMAFGGDADDATAAAIWRAARDAGVNL
                     FDTADVYNEGRSEQVLGRLMRDDRDQIVVASKAYFPTGKGANDRGASRYHLVRAVEAS
                     LRRLATDRIDLYYLHRFDDLAALDETLRGLEDLVRAGKILYPACSNFAAWQVAHALGL
                     QRLHGWAPLVAIQPMYNLVKRQAEVEILPMAQSLGLAVIPYSPTGGGLLTGKYSPDRR
                     PEHGRLTDNKMYAVRYGDPRYLTTAAQFAALAAELGHAPATLAVAWVASHPGVTSVLI
                     GGRNPEQLTPTLAAAQLVLDDATRARISALSAEPPPATDRNEEATAHNYGTR"
     gene            complement(1393..1959)
                     /locus_tag="VF516_16885"
     CDS             complement(1393..1959)
                     /locus_tag="VF516_16885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109411.1"
                     /translation="MSEVTLQDRERALENQFYDKENQEKLAAMKRKLDAQASKEELRK
                     ASGMTDDAVLDQLVALGLRGNTIAALSLVPLIQVAWADGTIQDNERTAILQGAHGKGL
                     EEGSDGYELLQRWLERQPGDELFSAWESYIKALASQLNDEQNRLLKNQIVGFAKLVAT
                     AAGGILGFGKVSASEEKVLHRIEAAFHR"
     gene            2085..3656
                     /locus_tag="VF516_16890"
     CDS             2085..3656
                     /locus_tag="VF516_16890"
                     /inference="COORDINATES: protein motif:HMM:NF021524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 family protein"
                     /protein_id="HEX8109412.1"
                     /translation="MGLWLVPMAILGGIALLVFAVLYALDHHVTGGISDPHPDSAVGR
                     YVRFEPTLISDALSGLAGMTAAVLGIVITVVSLLVQLTAERYTGVAQMFLRDRRNVVV
                     MAYYVVTCVVGVALSLSLHNEFVPRAAVFSMMCATAFGLIMMLPYFAYVFRFLEPVNL
                     VARIEREAAIDVASAATTRDPDAIVDGQAPALAALEELTDITSNSISGKDKIIASRAV
                     DAIRDFVTGYLAVKGRADPRWFAIGDRIRDNPDFVAMDPESLRELEADRTWLEWKALR
                     QYLAISAEAQGEMPDINYLIAIDTRYVGEAALARGDRAVVELAFRFMNSYLRAALNAR
                     AVRTAYNVMNQYRLLVEAMIRGGADDLALDGVRHMIYYGRTSYDMQLGFVTETVAYDV
                     SALCEFAHIQGQSGQGRAGLDDEMLAMFLELDQPLRVKRQESGLQGVRKAQVKLACYY
                     LAAGITDRAERIAADMTGEDRDRLRSIREQLAGVESKKYWEIIDRGRNFDYMPPRQKE
                     QMDRFFAMLDHDAVR"
     gene            3668..>4166
                     /locus_tag="VF516_16895"
     CDS             3668..>4166
                     /locus_tag="VF516_16895"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8109413.1"
                     /translation="MSRTLVMGDPQAPFGKLLEVLDRHQALDGDRLASDVTLVSIGDH
                     FDYDSANWQTAGEDGLKFLRWLASHDAAQVKLLLGNHDVSRVMELVAITDERFAAARA
                     FARVLDGDPARDARFRAAFPELPTAGLAGRDFASFSVEQRALVMELLLAGRFDLALVG
                     ELPDGR"
BASE COUNT          554 a         1456 c         1486 g          670 t
ORIGIN      
        1 gccgacgtgg tgtgaaacac ctggacgagg aagcggacgt cttccggtcg ctgcaggacg
       61 acgtcttcgc ggctcacggc ggtcatttcc acgagatgct tccagcgtac acgagccgcg
      121 ccggtgcgtg gtgccccatg cccacggttt ggcgcgctcc ccgatgcacg acctttctac
      181 gcgcgcccgt cgggcgggct tcccgggatc cgtgaacgga catgcggggt taaatgcccc
      241 tggcggctgc tgtattcccg gcgcgcggct cggtgctcac gccgcggcaa gcgatgccac
      301 gccggcggcg agcgttcgat gcaatcgaga ccggcgtgct gcgatccgtc cgagcgtacg
      361 gcggccgatc ggcatgggca ggccagctcg cggttgccgg ccgtcgcggc taccgcgtgc
      421 cgtagttgtg cgcggtggct tcctcgttgc gatcggtcgc cggcggcggc tcggccgaca
      481 gcgccgagat ccgcgcccgg gtcgcgtcgt cgagcaccag ctgggcggcc gccagcgtcg
      541 gggtgagctg ctcggggttg cggccgccga tcagcacgct ggtcacgccg gggtgcgacg
      601 cgacccacgc gaccgccagc gtcgccgggg cgtggccgag ctcggccgcg agcgcggcga
      661 actgggccgc ggtggtcagg tagcgcggat cgccgtagcg caccgcgtac atcttgttgt
      721 cggtcagccg gccgtgctcg ggccgccggt ccgggctgta cttgccggtg agcaggccgc
      781 cgccggtcgg gctgtacggg atcacggcga gcccgagcga ctgcgccatc ggcaggatct
      841 cgacctcggc ctggcgcttg accaggttgt acatcggctg gatcgcgacc agcggcgccc
      901 agccgtgcag ccgctggagg ccgagcgcgt gggcgacctg ccaggccgcg aagttcgagc
      961 acgccgggta gaggatcttg ccggcgcgca ccaggtcctc gaggccgcgc agggtctcgt
     1021 cgagcgcggc caggtcgtcg aaccggtgca ggtagtacag gtcgatccgg tcggtggcga
     1081 gccggcgcag gctggcctcg acggcgcgca ccaggtggta gcgcgacgcg ccccggtcgt
     1141 tggcgccctt gccggtcggg aagtacgcct tgctcgcgac cacgatctgg tcgcggtcgt
     1201 cgcgcatcag ccggccgagc acctgctcgg agcggccctc gttgtagacg tcggcggtgt
     1261 cgaacaggtt gacgccggcg tcgcgcgcgg cgcgccagat cgcggccgcg gtggcgtcgt
     1321 cggcgtcgcc gccgaacgcc atcgtgccga gcgcgacccg cgagatcttg accccggtgg
     1381 tgccgagcag cttcatcggt gaaacgccgc ctcgatccgg tgcaggacct tctcctcgga
     1441 ggcggacacc ttgccgaacc cgaggatgcc gccggccgcg gtggcgacca gcttggcgaa
     1501 cccgacgatc tggttcttga gcagccggtt ctgctcgtcg ttgagctgcg acgcgagcgc
     1561 cttgatgtag gactcccagg ccgagaacag ctcgtcgccc ggctggcgct cgagccagcg
     1621 ctggagcagc tcgtagccgt cgctgccctc ctccaggccc ttgccgtggg cgccctggag
     1681 gatcgcggtg cgctcgttgt cctggatcgt gccatcggcc cacgcgacct ggatcagcgg
     1741 gaccagcgac agcgcggcga tcgtgttgcc gcgcaggccg agcgcgacca gctggtcgag
     1801 cacggcgtcg tcggtcatgc ccgacgcctt gcgcagctcc tccttggacg cctgggcatc
     1861 gagcttgcgc ttcatcgcgg cgagcttctc ctggttttcc ttgtcgtaga actggttttc
     1921 gagcgctcgc tcgcggtctt gcagggtcac ttcgctcacg acggctcctt gggtggaccc
     1981 ttccaaccct aacgcagttt cgccccctat cccacccggt ggggcgctga ggtaacgtcg
     2041 gccgcgtgag ctcacggccg cgccgtcgtc cgcaccgcag cgggttgggt ctctggctgg
     2101 tcccgatggc gatcctcggc ggcatcgcgc tcctggtgtt cgccgtgctg tacgcgctcg
     2161 accaccacgt cacgggtggg atctcggatc cccacccgga cagcgcggtc ggccgctacg
     2221 tgcggttcga gccgaccctg atctccgatg cgctgtccgg gctcgccggg atgaccgccg
     2281 cggtgctcgg catcgtgatc accgtggtgt cgctgctcgt ccagctcacc gcggagcgct
     2341 acaccggcgt ggcgcagatg ttcctgcgcg atcgccgcaa cgtcgtggtg atggcgtact
     2401 acgtggtgac ctgcgtggtc ggcgtcgcgc tcagcctgtc gctgcacaac gagttcgtgc
     2461 cgcgcgccgc ggtgttctcg atgatgtgcg cgaccgcgtt cggcctgatc atgatgctgc
     2521 cgtacttcgc ctacgtgttc cggttcctcg agccggtcaa cctggtcgcc cggatcgagc
     2581 gcgaggccgc gatcgacgtc gcgagcgcgg cgaccacccg cgatcccgac gcgatcgtcg
     2641 acggccaggc cccggcgctg gccgcgctcg aggagctgac cgacatcacg tcgaactcga
     2701 tctcgggcaa ggacaagatc atcgcgagcc gcgcggtcga cgcgatccgc gacttcgtca
     2761 ccggctacct cgcggtcaag ggccgcgccg atccgcggtg gttcgcgatc ggcgaccgga
     2821 tccgcgacaa cccggacttc gtcgcgatgg atcccgagtc gctgcgcgag ctcgaggccg
     2881 accggacctg gctcgagtgg aaggcgctgc gccagtacct ggcgatctcc gccgaggccc
     2941 agggcgagat gccggacatc aactacctga tcgcgatcga cacccggtac gtcggcgagg
     3001 ccgcgctcgc gcgcggcgac cgcgcggtgg tcgagctcgc gttccggttc atgaacagct
     3061 acctgcgcgc cgcgctcaac gcccgcgcgg tgcgcaccgc gtacaacgtg atgaaccagt
     3121 accgcctgct ggtcgaggcg atgatccgcg gcggcgccga cgacctcgcg ctcgacggcg
     3181 tgcgccacat gatctactac ggccggacca gctacgacat gcagctcggg ttcgtcaccg
     3241 agaccgtggc gtacgacgtg tcggcgctgt gcgagtttgc ccacatccag ggccagtcgg
     3301 ggcagggccg ggccgggctc gacgacgaga tgctcgcgat gttcctcgag ctcgatcagc
     3361 cgctgcgggt caagcgccag gagtccgggc tgcagggcgt ccgcaaggcc caggtcaagc
     3421 tcgcctgcta ctacctcgcc gccgggatca ccgaccgcgc cgagcggatc gccgccgaca
     3481 tgaccggcga ggaccgcgac cggctgcgct cgatccgcga gcagctggcc ggcgtcgagt
     3541 ccaagaagta ctgggagatc atcgatcgcg gccgcaattt cgactacatg ccgccgcgcc
     3601 agaaggagca gatggatcgc ttcttcgcga tgctcgacca cgacgcggtg cgctaagctg
     3661 gcgagcgatg tcccgcacgc tggtgatggg cgatccacag gcgccgttcg gcaagctcct
     3721 ggaggtgctc gaccggcacc aggcgctgga cggcgaccgc ctggcgagcg acgtcacgct
     3781 ggtgtcgatc ggcgaccact tcgactacga ctccgcgaac tggcagaccg ccggggagga
     3841 cggcctgaag ttcctgcgct ggctggcgag ccacgacgcc gcgcaggtca agctgctgct
     3901 cggcaaccac gacgtgtcgc gggtgatgga gctcgtggcg atcaccgacg agcggttcgc
     3961 ggcggcccgc gcgttcgccc gcgtgctcga cggcgatccg gcgcgcgatg cccggttccg
     4021 cgcggcgttc cccgagctgc cgaccgccgg gctcgccggc cgcgacttcg cgtcgttctc
     4081 ggtcgagcag cgcgcgctgg tcatggagct cttgctcgcg gggcggttcg atctcgcgct
     4141 ggtcggcgag ctgcccgacg gccgcg
//
LOCUS       DASVOR010000861         1710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_9064,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000861 DASVOR010000000
VERSION     DASVOR010000861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9064"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VF516_16900"
     CDS             <1..336
                     /locus_tag="VF516_16900"
                     /inference="COORDINATES: protein motif:HMM:NF015019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEX8109414.1"
                     /translation="TELHAATVVGDTVGDPFKDTSSVALNPVIKFTTLFGVLAVGLAL
                     ELAAKAQEAGSPTFNYVRYAVAVLGLLIALTFAYRSFYGMRIGKAAEESPAQSSAPKV
                     EPKADSAAS"
     gene            433..>1710
                     /gene="hutU"
                     /locus_tag="VF516_16905"
     CDS             433..>1710
                     /gene="hutU"
                     /locus_tag="VF516_16905"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR01228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEX8109415.1"
                     /translation="METIRAPRGSALSCKGWPQEAALRMLMNNLDPDVAERPADLVVY
                     GGTGKAARSWDDYHTIVRELRRLDSDHTLLVQSGRAVGVFRTHEEAPRVLIANANLVG
                     NWATWEHFRELERKGLMMYGQMTAGSWIYIGSQGIVQGTYETFAAAGKTHWNSDLAGR
                     FVLSGGLGGMGGAQPLAATMAGGVFLGVEIDPSRVERRLATRYLDELAPDLDTALRRV
                     DEHRRARTARSIAVIGNAADVYAELVRRGVTPDLVTDQTAAHDPLVGYIPQGLSLAAA
                     AELRARDPDEYIRRARASMAVQVEAMVAMQRAGAHVFDYGNNLRAQAELGGARDAFRY
                     PGFVPAYIRPLFCEGKGPFRWAALSGDPDDIRATDEAVLDEIRDDPMLRRWMTMARER
                     IAFQGLPARICWLGYGQRHRVGLRFNELVATGKV"
BASE COUNT          236 a          606 c          631 g          237 t
ORIGIN      
        1 accgagctgc acgccgcgac cgtggtcggc gacaccgtcg gcgatccgtt caaggacacg
       61 tcgtcggtcg cgctcaaccc ggtgatcaag ttcaccacgc tgttcggcgt gctcgcggtc
      121 ggcctcgcgc tcgagctcgc ggccaaggcg caggaggcgg gctcgccgac cttcaactac
      181 gtgcgctatg cggtcgcggt gctcggcctg ttgatcgcgc tgaccttcgc ctaccgctcg
      241 ttctacggca tgcggatcgg caaggcggcg gaggagtccc cggcgcagtc gtcggcgccc
      301 aaggtcgagc ccaaggccga ctcggcggcg agctgagccg gcggcccgcg gagttgcggg
      361 tcggctgaag aagggcgcac tcaccgtgcg ccctttgcgg tctttttcgt cccggggtgg
      421 cacgatgccg acatggagac gattcgtgcg ccgcgcggta gcgcgctgag ctgcaagggc
      481 tggccgcagg aggccgcgct gcggatgctg atgaacaacc tcgacccgga cgtcgccgag
      541 cggccggccg acctggtggt ctacggcggc accggcaagg cggcgcgcag ctgggacgac
      601 taccacacca tcgtgcgcga gctgcgccgg ctggacagcg accacacgct gctggtccag
      661 tccgggcgcg cggtcggcgt gttccgcacc cacgaggagg cgccgcgcgt gctgatcgcg
      721 aacgccaacc tggtcggcaa ctgggcgacc tgggagcact tccgcgagct cgagcgcaag
      781 ggcctgatga tgtacggcca gatgaccgcg ggctcgtgga tctacatcgg ctcgcagggc
      841 atcgtgcagg gcacctacga gacgttcgcc gccgccggca agacccactg gaacagcgac
      901 ctcgccggcc ggttcgtgct gtcgggcggc ctgggcggga tgggcggggc ccagccgctg
      961 gccgcgacca tggcgggcgg cgtgttcctc ggcgtcgaga tcgatccgag ccgggtcgag
     1021 cgccggctcg cgacccgcta cctcgacgag ctcgcgcccg atctcgacac cgcgctgcgc
     1081 cgggtcgacg agcaccgccg ggcgaggacc gcgcgctcga tcgccgtgat cggcaacgcc
     1141 gccgacgtct acgccgagct ggtccgccgc ggcgtgacgc cggacctggt caccgaccag
     1201 accgcggccc acgatccgct ggtcggctac atcccgcagg gcctgagcct ggccgcggcc
     1261 gccgagctgc gcgcacgcga ccccgacgag tacatccggc gggcgcgggc gtcgatggcg
     1321 gtccaggtcg aggcgatggt cgcgatgcag cgcgccggcg cccacgtgtt cgattacggc
     1381 aacaacctgc gcgcccaggc cgagctcggc ggcgcacgcg atgcgttccg ctaccccggc
     1441 ttcgtgccgg cctacatccg gccgctgttc tgcgagggca aggggccgtt ccgctgggcg
     1501 gcgctgtcgg gcgatcccga cgacatccgc gcgaccgacg aggcggtgct cgacgagatc
     1561 cgcgacgatc cgatgctgcg gcggtggatg acgatggcgc gcgagcggat cgcgttccag
     1621 ggcctgccgg cgcggatctg ctggctcggc tacggccagc gccaccgggt cggcctgcgg
     1681 ttcaacgagc tggtcgccac cggcaaggtc
//
LOCUS       DASVOR010000862         1818 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_329999,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000862 DASVOR010000000
VERSION     DASVOR010000862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329999"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1373
                     /locus_tag="VF516_16910"
     CDS             <1..1373
                     /locus_tag="VF516_16910"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEX8109416.1"
                     /translation="LDAFWTELRAGRCAITEVPAGRWRADLLYRPQLEAGKTISKWGG
                     FLDAIEDFDPGYFAMTDEEATCLDPAVRLMLEGIEACLADAGYTAPEVGGRAVGVFAG
                     ARVSDYWRRTGSRTGSAGFGADQNFIAARIAHHLDLGGPCFVVDSACSSSLVAVQLAA
                     RSLLAGESELAFAAGVEVLLDERPYLEFSAARALSPSGRCRVFDERADGFVPGEGCGV
                     VLLKRLDRALRDGDRIRAVIEAVAVNNDGRTVGLTTPNPAAQTAVILRALEQAGRTAR
                     DIALIEAHGTGTMLGDPIELRALTDAFASHALLPGSCAIGSVKSNLGHLLSAAGIAGL
                     LKGMLSLERGEIPPTLFCERPNPRFEFARSPFYPVVGLQRWPDDRPRVAGVSAFGLGG
                     TNAHLILAAHEPASHAVPVRHPLAPPQLRRRRLWLDREPVIARPEIAPHPLIASILDL
                     QFDGAS"
     gene            1376..1615
                     /locus_tag="VF516_16915"
     CDS             1376..1615
                     /locus_tag="VF516_16915"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8109417.1"
                     /translation="MDPHAIFEAIRRNVIAVVPEVAAEQIAPDCTLSDLGLNSLDRAE
                     VITLTMADLAISVPVHEFHRGNSIAALVEVMRRYS"
     gene            1612..>1818
                     /locus_tag="VF516_16920"
     CDS             1612..>1818
                     /locus_tag="VF516_16920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109418.1"
                     /translation="MIATITGVGVTCSLGASADAFEQALRRGRSGIAADPWGGDGPPF
                     GAVIDGFTLAGALAARSALPRALLE"
BASE COUNT          227 a          662 c          664 g          265 t
ORIGIN      
        1 atctcgacgc gttctggacc gagctgcgcg ccggccggtg cgcgatcacg gaggtcccgg
       61 cggggcggtg gcgcgccgac ctgctgtacc ggccgcagct cgaagccggc aagacgatca
      121 gcaagtgggg tggcttcctc gatgcgatcg aggacttcga cccggggtac ttcgcgatga
      181 ccgacgagga ggcgacctgc ctggacccgg ccgtgcgcct catgctcgaa ggcatcgagg
      241 cgtgcctcgc cgacgccgga tacaccgcgc cggaggtcgg cggtcgcgcg gtcggcgtgt
      301 tcgccggagc gcgggtatcg gactactggc gccggaccgg ctcgcggacc ggcagcgccg
      361 gcttcggcgc ggaccagaac ttcatcgcgg cgcggatcgc gcaccacctc gacctcggcg
      421 ggccgtgctt cgtggtcgac agcgcgtgct cgtcgtcgct ggtcgcggtc cagctcgcgg
      481 cgcgcagcct gctcgccggc gagtccgagc tcgcgttcgc ggccggcgtc gaggtgctgc
      541 tcgacgagcg gccctacctc gagttcagcg ccgcgcgcgc gctgtcgccg tcggggcgct
      601 gccgggtgtt cgacgagcgc gccgatggct tcgtccccgg cgagggctgc ggcgtcgtcc
      661 tgctcaagcg gctggatcgc gcgctgcgcg acggcgaccg catccgcgcc gtgatcgagg
      721 ccgtcgcggt caacaacgac ggccgcaccg tcgggctgac cacgccgaac ccggcggcgc
      781 agaccgcggt gatcctgcgc gccctcgagc aagccgggcg caccgcgagg gacatcgcgc
      841 tgatcgaggc gcacgggacc ggcacgatgc tcggcgatcc gatcgagctg cgcgcgctca
      901 ccgacgcgtt cgcgagccac gccctgctcc cggggagctg cgcgatcgga agcgtcaagt
      961 cgaacctcgg ccacctgctg agcgcggcgg gcatcgccgg cctgctcaag gggatgctct
     1021 cgctcgagcg cggcgagatc ccgccgacgc tgttctgcga gcgccccaac ccgcgcttcg
     1081 agttcgcgcg ctcgccgttc tacccggtcg ttggcctgca gcgctggccc gacgaccggc
     1141 cgcgggtcgc gggcgtgagc gcgttcggtc tcggaggcac caacgcgcac ctgatcctcg
     1201 ccgcgcacga gccggcgagc cacgcggtgc cggtgcgcca cccgctcgcg ccgccgcagc
     1261 tccggcgccg ccgcctgtgg ctggatcgcg agccggtcat cgcccggccc gagatcgcgc
     1321 cgcaccccct gatcgcatcg atcctcgatc tccagttcga cggagcttcc tgatcatgga
     1381 cccgcacgcc atcttcgaag cgatccggcg caacgtgatc gcggtcgtcc ccgaggtcgc
     1441 ggccgagcag atcgcgccgg actgcacgct gtcggatctc gggctcaaca gcctcgatcg
     1501 tgccgaggtg atcacgctca cgatggcgga cctcgcgatc tcggtgccgg tccacgagtt
     1561 tcatcgcgga aacagcatcg cggcgctggt cgaggtgatg cggaggtact cgtgatcgcg
     1621 acgatcaccg gcgtgggggt gacctgctcg ctcggcgcga gcgccgacgc cttcgagcag
     1681 gcgttgcgcc gaggtcgctc ggggatcgct gcggatccgt ggggcggcga tggcccgccg
     1741 ttcggtgcag tgatcgacgg cttcacgctc gccggggcgc tcgcggcgcg atccgcgttg
     1801 ccccgggcgc tgctcgag
//
LOCUS       DASVOR010000863         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_271356,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000863 DASVOR010000000
VERSION     DASVOR010000863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271356"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(753..825)
                     /locus_tag="VF516_16925"
     tRNA            complement(753..825)
                     /locus_tag="VF516_16925"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(790..792),aa:Phe,seq:gaa)
     gene            1083..>2045
                     /locus_tag="VF516_16930"
     CDS             1083..>2045
                     /locus_tag="VF516_16930"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX8109419.1"
                     /translation="MSSITACQLRSQYLAFFASRGHAAIPSASLVPANDPSVLFTTAG
                     MHPLAPYLLGQPHPAGRRLVDVQECVRTGDIDAVGDDTHLTWFEMLGNWSLGDYGRDE
                     TIAWSFEFLTRELGLAVERLGVTCFAGDEEVAEDRASAEIWRRLGMPAARIARLGRDD
                     NWWGPVGATGPCGPDTEMFYWVDGSPPPEVLDVRDRRWVEIWNNVFMSHAKTAGGEIV
                     ALPRVSVDTGMGVERTLVALNGLGSVYDVDTLRPLRDELCELASAPHERELRVLADHV
                     RAACMVIADGVAPSNKDRGYVLRRLVRRGLVAARRLGLPGDWHRR"
BASE COUNT          306 a          645 c          806 g          288 t
ORIGIN      
        1 gaccatgtgg cgcttgagga tcgcgacctt ctgctcggtg gtgaagtggc ggcgggaacg
       61 cttggaactc gacatcggtc tcctggcagc cgggtgcgta ccacggcggt ctgaccgtca
      121 cgttccagcc gaggcagaac agatgctgga ggagatccac gaggacgcgc ggaccgggcc
      181 tgtcgtgccc tcatggtcga acgtccgccg ggacctcaac gcgctggtcc gcgcgtacaa
      241 caaggcccag gcgaaggctg cagcggcgga gaagcgtccg gcgccgccgg cgatgcgcaa
      301 catcagcccc aacgatctgc ggcgcacgtt cgccagctgg gtcaagcaag cgggcgttga
      361 ctcgatggtg gtcgcgaagc tgctggggca caccacctcg cgcatggtcg agctggtgta
      421 cggccacctg aacgacgcca cgaccagcgc agcgatcacc aagctgccgg cgccgcccgc
      481 ccccagcctc gccgagccgg ccgcgcgtgc ggtgaccgtt ccggtacggc ggcgacgcgg
      541 cgtcgcgggc gaagcgccga ccttaaagct ggccggtgac aatgccgctg cacccccacc
      601 gagcgagccg gtgccgcggt ggcggagtcg cgccgggcgc gtcgatccag cccccgggag
      661 cggtagtaaa tgggtagtaa gacccctgcg aaacgagcga ctagagagac ggatgagaca
      721 acccgagtct cagtcttcgt cggaagttac ggtgcccagg gtcggaattg aaccaacgac
      781 acgcggattt tcagtccgct gctctaccaa ctgagctacc tgggcggcgg aaggggagac
      841 tacacagggc gggtcgcgct ggcaaccgga ccgggtgcga tcgtggcggg cgtttgcgcg
      901 caggaagggc gcgccgtata gtggcagcat gtcgatcgtt cgtgccgcgg ctgcctttgc
      961 gccggctatc gccggtggtg gccccgccgc gcgcgtgaac tgacgacgct cgctgccggc
     1021 cgccacccaa cggggcggcc gacgacgtcg tggcgtggcc gctcggaaag gaccgaactg
     1081 ccatgtcatc gatcaccgct tgccagcttc gctcgcagta cctcgcgttc tttgcgtcgc
     1141 gcggccacgc cgcgatcccg tcggcgtcgc tggtgccggc gaacgatccg agcgtgctgt
     1201 tcacgacggc cgggatgcac ccgctcgcgc cgtacctgct ggggcagccg cacccggcgg
     1261 ggcggcggct ggtggacgtg caggagtgcg tgcggaccgg ggacatcgac gcggtcgggg
     1321 acgacacgca cctgacgtgg ttcgagatgc tcggcaactg gtcgctcggg gactacgggc
     1381 gcgacgagac gatcgcgtgg agcttcgagt tcttgacccg cgagctcggg ctggccgtgg
     1441 agcggctcgg ggtgacgtgc ttcgccgggg acgaggaggt ggcggaggat cgcgcgtcgg
     1501 cggagatctg gcggcggctc gggatgccgg cggcgcggat cgcgcggctc gggcgcgacg
     1561 acaactggtg gggtccggtg ggggcgacgg ggccgtgcgg gccggacacc gagatgttct
     1621 actgggtgga cggctcgccg ccgccggagg tgctcgacgt gcgcgaccgg cgctgggtcg
     1681 agatctggaa caacgtgttc atgagccacg cgaagacggc aggcggggag atcgtggcgc
     1741 tgccgcgggt gagcgtcgac acggggatgg gagtggagcg cacgctggtg gcgctgaacg
     1801 ggctgggctc ggtgtacgac gtcgacacgc tgcggccgct gcgcgacgag ctgtgcgagc
     1861 tggcgagcgc gccgcacgag cgcgagctgc gggtgctggc ggatcacgtg cgcgcggcgt
     1921 gcatggtgat cgccgacggg gtggcgccga gcaacaagga ccgcggctac gtgctgcggc
     1981 ggctggtgcg gcgcgggctg gtggcggcgc gccggctcgg gctgccgggg gactggcacc
     2041 ggcgc
//
LOCUS       DASVOR010000864         2109 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_330006,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000864 DASVOR010000000
VERSION     DASVOR010000864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330006"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..226
                     /locus_tag="VF516_16935"
     CDS             <1..226
                     /locus_tag="VF516_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109420.1"
                     /translation="TAAAYDCYDSVIGRRAMDYRDKLAYLSRHRGRTLGLGLAIAGLL
                     LVPGLNLLALALGSAGATVADHAIRRGAHR"
     gene            complement(227..1201)
                     /locus_tag="VF516_16940"
     CDS             complement(227..1201)
                     /locus_tag="VF516_16940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109421.1"
                     /translation="MCHLRDLPCAIAVIGLAAGGGCGGVNNGHGPPDAAIDARPPDSA
                     ALPDAAAARCDPSKPFGMPALIPNINSDVRDQGAELIDDLTIIFGSDRSPPGLYIATR
                     ATPTSPFGTPAPLTAINATPGATGVAGPTLTADGLTLYYAVTTAQDPGDIYVTHRNSR
                     ADAFAAGTPVTGLNSPTTDLDPFISADGSTLYFDSGRLTSVSLRLYAAVRQASGSFAA
                     PQPLTAFNSQDAAIVDGHPVLSPDGLTLYWSSTRTDGGAQGLTDIWKASRTSTAGDFG
                     AAVRVPELSSAKGESVSWVSPDNCMVLLQSDRPGGPGLQDIYQAVRPL"
     gene            complement(1333..>2109)
                     /locus_tag="VF516_16945"
     CDS             complement(1333..>2109)
                     /locus_tag="VF516_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109422.1"
                     /translation="WIEGVGVWWGDGARAWQLARLATAVALELAFVALAIRQLRRATG
                     DELAEDRLARPLAQLLPPRAARLISFELVIMTLALRFPFDGRRPSPPGFTYHRDSPLR
                     LFLPVLPLLGAGDVALLELVLLPHAPGWLRIVVHVLAVYGLLWVVGLWSSLRSRPHVI
                     GDGRAVLHRGVLCRVEVPLAAIASVAPVPVFSDDWKRRAYRKGAIRLDIAGPPALELR
                     LREPVRPVGVLGPGRPGDRLLVSVDDLEGFQRAIAAGSGA"
BASE COUNT          325 a          751 c          768 g          265 t
ORIGIN      
        1 caccgccgcg gcgtacgatt gttacgattc ggtgatcggc cggcgcgcga tggactatcg
       61 cgacaagctc gcctacctgt cgcggcaccg cggccgcacg ctcggcctgg ggctcgcgat
      121 cgccggcctg ctgctggtcc ccggcctcaa cctgctcgcg ctcgcgctcg gcagcgccgg
      181 cgcgacggtc gccgatcacg cgatccggcg cggcgcgcac cgctgatcac agcggccgga
      241 ccgcctggta gatgtcctgc aggccggggc cgccgggacg gtcggactgg agcaggacca
      301 tgcagttgtc cggtgagacc cacgacacgg actcgccctt cgccgagctg agctccggaa
      361 cccggaccgc cgcgccgaag tcgcccgccg tggacgtccg cgacgccttc cagatgtcgg
      421 tgagcccctg ggcgcccccg tcggtccggg tcgagctcca gtacagcgtg agcccgtcgg
      481 ggctcagcac cgggtgaccg tcgacgatcg cggcgtcctg gctgttgaag gcggtcagcg
      541 gctggggtgc ggcgaacgag ccgctggcct ggcggaccgc ggcgtacagg cgcagcgaga
      601 cactggtgag gcgcccggag tcgaagtaca gcgtggatcc gtccgcggag atgaacggat
      661 cgagatcggt cgtgggcgag ttgaggccgg tgaccggagt gcccgcggcg aaggcatcgg
      721 cccggctgtt ccggtgggtc acgtagatat ctcccggatc ctgggccgtc gtgaccgcgt
      781 agtacagggt caagccatcg gcggtcagcg tcggcccggc cactccggtc gctccggggg
      841 tcgcattgat cgcggtgagc ggcgccggag tgccgaacgg gctggtcggc gtcgcgcggg
      901 tcgcgatgta cagcccgggc ggcgaacgat cactcccgaa gatgatcgtg aggtcgtcga
      961 tgagctccgc gccctggtct cggacatcgc tgttgatgtt ggggatcagc gccggcatgc
     1021 cgaacggctt gctcggatca cagcgcgcgg ccgccgcatc cggcagcgcc gccgagtccg
     1081 gcggcctcgc atcgatcgcc gcgtcgggcg ggccgtgacc attgttgacg ccgccgcacc
     1141 cgccgcccgc cgccaggccg atcaccgcga tcgcgcacgg cagatcccgc agatgacaca
     1201 tgtgcattcg tgatgttctc ccaggatatt ccaatgcatg ttatcggtgc gctccgagga
     1261 aatacaggaa tgccccagcc gcatgatgcg gcacctcgcg tgacgcaggt gacagcgcgg
     1321 agcccctgct gctcaggctc cagaccccgc tgcgatcgcg cgctggaacc cctcgaggtc
     1381 gtcgacggat accagcaggc gatccccggg ccgaccgggg ccgagcacgc cgaccgggcg
     1441 gactggctcg cgcagccgga gctcgagcgc cggcgggccc gcgatgtcga gccggatcgc
     1501 ccccttgcgg tacgcgcggc gcttccagtc gtcggagaac acgggcaccg gcgcgaccga
     1561 cgcgatcgcc gccagcggga cctcgacccg gcacagcacg ccgcggtgca gcacagcgcg
     1621 gccatcgccg atcacgtgcg gccgcgagcg aagcgaggac cacaacccca ccacccagag
     1681 caggccgtac accgcgagca cgtggacgac gatccgcagc caccccggcg cgtgcggcaa
     1741 gaggaccagc tcgagcagcg cgacatcgcc ggcgccgagc agcggcagga ccggcaagaa
     1801 caggcgcagc gggctgtcgc ggtggtaggt gaagccgggc ggcgaggggc gccgcccgtc
     1861 gaacggaaac cggagcgcca gcgtcatgat gaccagctcg aacgagatca gccgggcggc
     1921 gcgcggcggc agcagctggg cgagcggacg ggcgagccgg tcctcggcca gctcgtcgcc
     1981 cgtggcgcgc cggagctggc ggatcgcgag cgcaacgaat gccagctcga gagcgacggc
     2041 cgtggcgagc cgtgccagtt gccaggccct ggcgccgtcg ccccaccaca cgcccacgcc
     2101 ctcgatcca
//
LOCUS       DASVOR010000865         2800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_66950,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000865 DASVOR010000000
VERSION     DASVOR010000865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2800
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66950"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..555)
                     /locus_tag="VF516_16950"
     CDS             complement(73..555)
                     /locus_tag="VF516_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8109423.1"
                     /translation="MPVDSHTYYRTYGPMVLRRCRKLLGEEQAARDAMHDVFVQVLSR
                     ADELADQAPSSLLFRIATNVCLNRLRTRRRRPEDGDPELLVQIAEETDPAARSAARAA
                     LDALFRHEPEDTALIAVLHLHDRMTLQEVAAEVGMSVSGVRKRLQKLRTKLHALEVSP
                     "
     gene            1515..1712
                     /locus_tag="VF516_16955"
     CDS             1515..1712
                     /locus_tag="VF516_16955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX8109424.1"
                     /translation="MAEGTIKRLTDKGFGFISNGSGKDMFFHMSAVEGTRFEDLREGQ
                     KVTYNEGHGPKGPRAENVRVV"
     gene            complement(1709..2332)
                     /locus_tag="VF516_16960"
     CDS             complement(1709..2332)
                     /locus_tag="VF516_16960"
                     /inference="COORDINATES: protein motif:HMM:NF017771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HEX8109425.1"
                     /translation="MEKPRIVSAEEWRRARDELLQAEKEATRAMDALAAQRRRLPMMR
                     FDASYEFEAPAGPTTLLGLFEGRRQLVVYQFMDLGPDRFCPGCTKFTSSVTDLERLAE
                     RGVSWATVSNMPLAQIEALRARMGWAMPFVSSRGTPFADDCGAGRGFMLSVFLRDGDE
                     VYRTYCTTQRGVDRLVFVHNILDLTPYGRQEDWEDSPTGWPQRPTYG"
     gene            complement(2433..2564)
                     /locus_tag="VF516_16965"
     CDS             complement(2433..2564)
                     /locus_tag="VF516_16965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109426.1"
                     /translation="MTARTLFESAVPELRAVVGEVAGRNDQDGVMRWLAAALAAGRV"
BASE COUNT          406 a         1058 c          901 g          435 t
ORIGIN      
        1 cgatgcggtc gcggctcgcg gggggaacct cgtcgagcgc cgcgcgttcg acgagccagt
       61 cgggtaggga cgtcacggtg aaacctccag ggcatggagc ttggttcgga gcttctgcag
      121 ccgcttgcgc acccccgaca ccgacatccc gacctcggcc gccacctcct gcagcgtcat
      181 tcgatcgtgc aggtgcagca cggcgatcag cgccgtgtcc tcgggctcgt ggcggaacag
      241 cgcgtccagc gccgcccgcg ccgcgctgcg cgccgccgga tccgtctcct cggcgatctg
      301 caccagcagc tccggatcgc cgtcctccgg ccgccgccgc cgcgtccgca gccggttgag
      361 gcagacattg gtggcgatcc ggaacagcag cgacgacggc gcctgatcgg cgagctcatc
      421 ggcgcggctg aggacctgga cgaagacgtc gtgcatggcg tcgcgtgcgg cttgttcctc
      481 gcccagcagc ttccggcacc gccggagcac catcggaccg taggtgcggt aatacgtgtg
      541 ggaatcgacg ggcacggcag ctgaggggcg ccggtgtaac accgcagccc cccaaaagtg
      601 cccctggcct ttttgccccg ccgcgcccgc ccgcgccggc tcgccacccg gcgggccgcc
      661 ggtgtgcgac gtggtgcgcg atcgagcggg gagcccgcca tcaccctccc tggtccggcg
      721 ccgtggtacc cgatcgatcc tggcgcccgc catcaccctc cctggtccgg cgccgtggta
      781 cgccatcgat cctggcgccc gccatcaccc tccctggtcc ggcgccatgg tttcgcgatc
      841 gacccaggcg cccgacgtta ttcgggccgt ttcggcctcg tggagccggg cgtcgcggag
      901 cccgcctgtg aggcgaccgc tgcagcaggc tgcgcgctcg aggttggccc ggcgctcgac
      961 gttggctcag cgctcgaggt cgcctcgccg gccggctcat ccccctcgat cccctcgatc
     1021 ggcggcccct tgccggaccg cggtgcggca cgccgtgcct ctcgtcgttc ggccttggca
     1081 cgcgccttgt ctttcagctt catctcgcgg tcgcgcttcg cgaacgcggg tcgttgggtc
     1141 gccatgtgtg cctcgcttcc gtggtaaacg atcgccggag catcgtcccc tccgaagcgg
     1201 ctcggagggc gacgttccgc acgcttcgtg gtcgcgctcg ccctcgtcga gaacgagccc
     1261 tcttcctacc acgaacgtcg ccgctgtacc atccggcaca cccgcgcccg ggcccggggg
     1321 cttccccggt cgcacgcgtg accgccctgc ggccgccggc gcctccgtac gaggtccgtc
     1381 catgtcatcc gacaatttgt caagggatcg ctcgacagcc ggcgcgatcc agggcaaaca
     1441 ggaccgctgg ccacgcgatc gaacggggcc gaatctctct ccccggcgcg gaaccacgaa
     1501 tgatggagac accgatggca gaaggtacga tcaagcgtct gaccgacaag ggctttggat
     1561 tcatcagcaa cggctcgggc aaggacatgt tctttcacat gtccgccgtc gagggcaccc
     1621 ggttcgaaga cctgcgcgaa ggtcagaagg tgacctacaa cgaaggccac ggacccaagg
     1681 gcccacgcgc cgagaacgtg cgcgtcgtct agccgtacgt cggccgctgc ggccaccccg
     1741 tcggcgagtc ctcccagtcc tcctgccggc catacggcgt caggtccagg atgttgtgga
     1801 cgaacaccag ccggtccacc ccgcgctgcg tcgtgcagta cgtccggtac acctcgtcgc
     1861 cgtcgcgcag gaacacgctc agcatgaacc cgcgcccggc cccacagtcg tccgcgaatg
     1921 gcgtccctcg tgacgacacg aacggcatcg cccaccccat ccgcgccctc agcgcctcga
     1981 tctgcgccag cggcatgttc gacaccgtcg cccagctcac cccgcgctcg gcaagccgct
     2041 ccaggtccgt cacgctgctc gtgaacttcg tgcaccccgg gcagaagcga tccggcccga
     2101 ggtccatgaa ctggtacacc accagctgcc tccggccctc gaacagcccg agcagcgtcg
     2161 tcggccccgc cggcgcctcg aactcgtagc tggcgtcgaa cctcatcatc ggcagccggc
     2221 gccgctgcgc cgccagcgcg tccatcgccc gcgtagcttc cttctcggcc tggagcagct
     2281 cgtcgcgcgc gcggcgccac tcctcggcgg acacgatcct cggtttctcc atgctccaag
     2341 catgccgcgg aacgcggcag ctggcgcccc gggccacgtc attcgacgac cgccgcgcgg
     2401 gtcgctcggg gatccgctga gcgccgcggg cgtcacaccc ggccggcggc gagcgccgcg
     2461 gcgagccagc gcatcacgcc gtcctggtcg ttgcgcccgg cgacctcgcc aaccaccgcg
     2521 cgcagctcgg gcaccgcgct ctcgaacagc gtccgagcgg tcatgctgaa gcccccttca
     2581 ttttcctcga accgggtcgg gtgcaatgta tgcagaaagg gtgtcgaggc actgcgacgc
     2641 cagggttggg ggaagagtac aagcggccag gtacgcttgg gcggcggtag ggttggtcct
     2701 cgcgttctgg accatcccgg cgagccagga gaagtcctcg gaggcgtatc tacgtcgctg
     2761 cctgccaacg acgtgcacgc caacctctcg aatgaaattg
//
LOCUS       DASVOR010000866         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_388324,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000866 DASVOR010000000
VERSION     DASVOR010000866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388324"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VF516_16970"
     CDS             complement(<1..436)
                     /locus_tag="VF516_16970"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8109427.1"
                     /translation="MGRLLDAARRFAAGRPEAGDLDAVHEAVAYFGRAVTRHFVDEEG
                     SVFPRLSTRRPELAAALAELSAEHPAQIAMQAEVAAAARDLDGDARPAAGKTLLDAAT
                     RLADAHRSHVGREDQIFATAHEALTAEDDAEIAAEMEMRRDRE"
     gene            583..1530
                     /gene="trxB"
                     /locus_tag="VF516_16975"
     CDS             583..1530
                     /gene="trxB"
                     /locus_tag="VF516_16975"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEX8109428.1"
                     /translation="MLENVVIIGSGPAAHTAAIYAARANLTPVLFEGFMAGGIAAGGQ
                     LTTTTDVENFPGFPEGIQGPELCERFRAQSARFGTRIYTETVDRVDLSQRPFRFWTEE
                     REGAARALIIATGATAKRDDIPGTRDHELWQKGVSACAVCDGALPMFRGKPLFVVGGG
                     DSAMEEGSFLSKFASKVHIVHRRDELRASAIMQQRARDNPKIELLLSHRVSVVEGGHG
                     VESVRVVDLKTGLERSMPAAGLFFAIGHVPNTRFLGGQVATDEQGYILTTPGTTLTSV
                     PGVFAAGDVQDKKYRQAITAAGSGCMAALEAEQYLSHNH"
     gene            1708..2847
                     /locus_tag="VF516_16980"
     CDS             1708..2847
                     /locus_tag="VF516_16980"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8109429.1"
                     /translation="MTREPQPPYRESGVTKILALLLLIVAGALVYTVIQQRRQPEHYV
                     SFEPRPIAQRPDDKLGADEQLTIDVFSKFSRSVVHITSLQTRRDRMTLDVAEIPQGTG
                     SGFVWDQDGHIVTNFHVVQLGNAASVTLNDNTTYPATIVGTAPDKDIAVLHIDAPPQK
                     LLPLPLGQSSTLKVGQKVLAIGNPFGLDQTLTTGVVSGLGREIKSVTQRSIFDVIQTD
                     ASINPGNSGGPLLDSSGRLIGINTAIYSPSGANAGIGFAVPVDTVNSIVPQLIKYHKV
                     KRPGLGIASVRDEIAMQNNIDGVVIGSVASGGAAEKAGLVGLSQDLDGTQLGDVIVKI
                     DGTDIHRSGDLFRVLDAHNVGDEVEVTVVNQGHRRTVKVTLQDVR"
     gene            2886..>3222
                     /locus_tag="VF516_16985"
     CDS             2886..>3222
                     /locus_tag="VF516_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109430.1"
                     /translation="MAPQPRPAWRNTLWRRARSLTAETAFQGLAGLASLHPAARPSRH
                     GIAVERDVVYGPDRRWHQLDLYRPVHRPGPWPVVFYVHGGAFHLLSKDTHWLMGLVFA
                     RHGYLVVNIS"
BASE COUNT          496 a         1148 c         1112 g          466 t
ORIGIN      
        1 cctcgcggtc gcggcgcatc tccatctccg cggcgatctc cgcgtcgtcc tcggcggtga
       61 gcgcctcgtg cgcggtggcg aagatctggt cctcgcggcc gacgtggctg cggtgggcat
      121 cggcgagccg ggtcgccgcg tcgagcaagg tcttgccggc ggcggggcgg gcgtcgccgt
      181 cgagatcgcg cgcggccgcg gcgacctcgg cctgcatggc gatctgcgcc gggtgctcgg
      241 cggacagctc ggcgagcgcc gcggcgagct cggggcggcg ggtcgacagc cgcgggaaca
      301 ccgagccttc ctcatcgacg aagtgccggg tgaccgcgcg cccgaagtac gcgaccgcct
      361 cgtgcacggc gtcgaggtcg ccggcctcgg ggcgccctgc cgcaaaccgc cgcgcggcgt
      421 ccagcagcct gcccatcacc tcgtcgtgac gccggtggca ccgctccagt tgcgcgaggc
      481 tggcaagctc cataccgggc aaggatccac gccccgaggt gctgcgacaa gcgccggacg
      541 tcgttgaaac aggggccgat cgcttgctag acctaaccgc ccatgctcga gaacgtcgtc
      601 atcatcggca gcggacccgc cgcccacacc gccgcgatct acgccgcgcg cgccaacctc
      661 acgccggtgc tgttcgaggg cttcatggcc ggcgggatcg cagccggtgg ccagctcacc
      721 acgacgaccg acgtcgagaa cttccccggc ttccccgagg gcatccaggg ccccgagctg
      781 tgcgagcggt tccgtgccca gtcggcgcgg ttcggcaccc ggatctacac cgagaccgtc
      841 gaccgcgtcg atctctcgca gcggccgttt cggttctgga ccgaggagcg cgagggcgcc
      901 gcgagggcgc tgatcatcgc gaccggggcg accgccaagc gcgacgacat cccggggacc
      961 cgcgatcacg agctgtggca gaagggcgtg tcggcgtgcg cggtgtgcga tggcgcgctg
     1021 ccgatgttcc gcggaaagcc gctgttcgtg gtcggcggcg gcgactcggc gatggaggag
     1081 ggaagcttcc tcagcaagtt cgcctccaag gttcacatcg tgcaccgccg cgacgagctg
     1141 cgcgcgtcgg cgatcatgca gcagcgcgcc cgcgacaacc ccaagatcga gctgctgctg
     1201 tcccaccggg tatcggtggt cgagggcggc cacggcgtcg agtcggtgcg cgtggtcgac
     1261 ctcaagaccg gcctcgagcg ctcgatgccc gcggccggcc tgttcttcgc gatcggccac
     1321 gtgcccaaca ccaggttcct cggcgggcag gtcgccaccg acgagcaggg ctacatcctg
     1381 accacgccgg gcaccaccct gacctcggtc ccgggggtgt tcgcggccgg cgacgtgcag
     1441 gacaagaagt accgccaggc gatcaccgcg gcgggctcgg gctgcatggc cgcgctggag
     1501 gccgagcagt acctcagcca taaccactga cagcgccgtg gcaagggcga tccgcgatcc
     1561 ccggccggtg cgagcggtgg acgcccgtgt gacgcggagc gcgtggttct tgcatcgcgt
     1621 gaggcgctcg catcaccggg aatatcgcga gccacggtca ggtagccctc cgtggaaggc
     1681 cgagccatgt cacaattcct ctaccccgtg acccgagagc cgcaaccgcc gtaccgcgag
     1741 tccggcgtga ccaagatcct cgcgctcctc ctgctgatcg tcgccggcgc gctggtgtac
     1801 accgtgatcc agcagcggcg gcagcccgag cactacgtgt cgttcgagcc ccggccgatc
     1861 gcgcagcggc ccgacgacaa gctcggcgcg gacgagcagc tgacgatcga cgtgttctcg
     1921 aagttctcgc gctcggtcgt acacatcacc agcctgcaga cccggcgcga ccggatgacg
     1981 ctcgacgtcg ccgagatccc gcagggcacc ggctcgggct tcgtgtggga ccaggacggc
     2041 cacatcgtca ccaacttcca cgtggtgcag ctcggcaacg ccgcgtcggt cacgctcaac
     2101 gacaacacga cgtaccccgc gaccatcgtc ggtaccgcac ccgacaagga catcgcggtg
     2161 ctccacatcg acgcgccgcc gcagaagctg ctgccgctgc cgctcggcca gtcgtcgacg
     2221 ctcaaggtcg gccagaaggt gctcgcgatc ggcaacccgt tcgggctcga ccagacgctg
     2281 accaccggcg tggtgtccgg gctcggccgc gagatcaaga gcgtcaccca gcgctcgatc
     2341 ttcgacgtga tccagaccga cgcgtcgatc aacccgggta actcgggcgg cccgctgctc
     2401 gacagctcgg gccggctgat cgggatcaac accgcgatct actcgccgtc gggcgccaac
     2461 gccggcatcg gcttcgcggt gccggtcgac accgtcaact cgatcgtccc gcagctgatc
     2521 aagtaccaca aggtcaagcg ccccgggctg ggcatcgcct cggtccggga cgagatcgcg
     2581 atgcagaaca acatcgacgg tgtggtgatc ggcagcgtcg cgtccggcgg tgccgctgaa
     2641 aaggccggcc tcgtcgggct gagccaggat ctggacggga cccagctcgg cgacgtcatc
     2701 gtcaagatcg acggcaccga catccatcgc tccggcgacc tgttccgggt gctcgacgcg
     2761 cacaacgtgg gcgacgaggt cgaggtgacg gtcgttaacc agggacatcg ccgcacggtc
     2821 aaggtaacgt tacaagacgt ccggtgagcc gagcttccgt agcagcaccc gaggggcttg
     2881 attcgatggc gccgcagccg aggccggcgt ggcgcaacac gctgtggcgc cgtgcgcgat
     2941 cgctgaccgc cgagaccgcg ttccaggggc tggcggggct cgcgtcgctg catccggcgg
     3001 cgcggccgtc gcgccacggc atcgcggtcg agcgcgacgt cgtctacggc ccggatcggc
     3061 gctggcacca gctcgacctg taccggccgg tgcaccgccc cgggccgtgg ccggtcgtgt
     3121 tctacgtcca cggtggagcg tttcacctgc tgtcgaagga cactcactgg ctgatgggcc
     3181 tggtgttcgc gcgccacggc tacctggtgg tcaacatctc ct
//
LOCUS       DASVOR010000867         2528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_301103,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000867 DASVOR010000000
VERSION     DASVOR010000867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2528
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301103"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VF516_16990"
     CDS             complement(<1..492)
                     /locus_tag="VF516_16990"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX8109431.1"
                     /translation="MPALVFIDGNELRAQEIRRILPGIPLAVEALQTGFSTSAPEPAQ
                     RARDKVLAHPVAAGCFAEAVDLTTLDGKSLRLELDSENENRFCKWWRETPARLHLVVA
                     LRRAPGAEIELVTGVCEGRIADQPAGPHVLGWDRLFVPAPDGAAGEGDKGDEEGDDGI
                     TLAQ"
     gene            complement(854..>2528)
                     /locus_tag="VF516_16995"
     CDS             complement(854..>2528)
                     /locus_tag="VF516_16995"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109432.1"
                     /translation="TTEAFATYGVIDDRTPGGAIAQPVALPGQVVPRFGGLEVITSST
                     HLAALTDAMLYLVHYPFECSEQRSSRILAIAALRDVLAAFHTKDMPSAAAMADSVRVD
                     LERLSQLQNPDGGVAFWDRGYPSVPYLSVYVASALAAARQKGFAVPGAILDRARPYLR
                     DIERHYPDFYPPDVRRAISAFALSTRKQLGDADVAKGKRLIAEAGGVDKLPLEADGWL
                     LGLFAQSPAAADERKAIVRHALNRVSETAGAASFTTGYGDGSYLLLASDRRVDAVMLD
                     ALIQDGADPDLISKLVTGLLGHRKAGRWLNTQENTFALVALDRYFQTYEKATPDFVAR
                     VWLGNDYAGEHAFRGRSTDNDQIAIAMKDVASHDRAQLTIQRAGTGRLYYRIGMTYAP
                     ASLQLAPADHGFVVERRYEAVDDPKDVTRAADGTWHIKAGARVRVRLSMVNDSRRYHV
                     ALVDPLPAGLEILNPALATTGPIPEDPKQASQRGPYWWWYGPWYEHQNLRDERAEAFA
                     SLVWEGVHGYDYVARATTPGSFVVPPPRAEEMYMPETFGRGASDRVIVE"
BASE COUNT          348 a          903 c          891 g          386 t
ORIGIN      
        1 ctgggcgagc gtgatgccgt cgtcgccctc ttcgtcgccc ttgtcgccct cacccgccgc
       61 gccgtccggc gccggcacga acagccggtc ccagcccagg acgtggggcc cggccggctg
      121 gtcggcgatc cggccctcgc acacgccggt gaccagctcg atctcggcgc ccggcgcgcg
      181 gcgcagcgcg accaccaggt ggagccgcgc cggggtctcg cgccaccact tgcagaaccg
      241 gttctcgttc tcggagtcga gctcgagccg caggctcttg ccgtcgagcg tcgtcaggtc
      301 cacggcctcc gcgaagcagc cggcggcgac cggatgggcc agcaccttgt cgcgcgcgcg
      361 ctgggcgggc tcgggcgcgc tggtcgagaa cccggtctgc agcgcctcca ccgcgagcgg
      421 aatgcccggc aagatccgcc ggatctcctg cgcccgcagc tcgttgccat cgatgaacac
      481 cagggccggc accgccgcac cctaccccaa tccgccggcg cgcgggggtc acgcgcccgg
      541 tcaagaagtg tcgatcaaag gttgggtgct tgcctcaggt gcgccaacgt ccgatggacg
      601 tcgacctgga ccttgacgtt gacggccgcc gttgaggttg acgtggccgg agacgttgag
      661 ccccatcgtc gaccttcacc ttgaccatcg gtcgcgattc ttcgacgagg atttgcatca
      721 gaaggtcgac gtacaagggc aagggtggag tcgacgtcca cgttgccgtt caggtcgacg
      781 ccttgggaca acgtcgaggt caacgtcaac gtcttcgcaa ggacgttccc ggtctttcat
      841 cgagacttcc tgttcactcg acgatcacgc gatcgctggc gccgcgcccg aacgtctcgg
      901 gcatgtacat ctcctcggcc ctgggcggcg gaacgacgaa gctgcccggc gtggtcgcgc
      961 gtgcgacgta gtcgtagccg tggacgcctt cccacacgag cgacgcgaac gcctcggcgc
     1021 gttcgtcgcg caggttctgg tgctcgtacc acggcccgta ccaccaccag tacggcccgc
     1081 gctggctcgc ctgcttgggg tcctcgggga tcggcccggt ggtcgccagc gccgggttca
     1141 ggatctcgag ccccgccggc agcgggtcga ccagcgcgac gtggtagcgc cggctatcgt
     1201 tgaccatcga cagccggacc cggacccggg ctccggcctt gatgtgccag gtcccgtcgg
     1261 ccgcgcgcgt cacgtccttc ggatcgtcga ccgcctcgta gcgccgctcg accacgaagc
     1321 cgtggtccgc cggcgcgagc tggagcgacg ccggcgcgta ggtcatgccg atccggtagt
     1381 acagccggcc ggtgccggcg cgctggatcg tcagctgcgc ccggtcgtgg ctcgccacgt
     1441 ccttcatcgc gatcgcgatc tggtcgttgt cggtcgaccg gccgcggaac gcgtgctcgc
     1501 cggcgtagtc gttgcccagc cagacccgcg cgacgaagtc cggcgtcgcc ttctcgtagg
     1561 tctggaagta gcgatcgagc gcgaccagcg cgaacgtgtt ctcctgggtg ttgagccagc
     1621 ggcccgcctt gcggtggccg agcagcccgg tcacgagctt ggagatcaga tcagggtccg
     1681 cgccgtcctg gatcagcgcg tcgagcatca ccgcgtcgac ccggcggtcg ctggccagca
     1741 gcaggtagct gccgtcgccg tagccggtgg tgaagctcgc ggcgccggcg gtctccgaca
     1801 cccggttcag cgcgtggcgc acgatcgcct tgcgctcgtc ggcggcggcc gggctctgcg
     1861 cgaacagccc gagcagccag ccgtcggcct cgagcggcag cttgtcgacg ccgccggcct
     1921 cggcgatcag ccgcttgccc ttggcgacgt cggcgtcgcc gagctgcttg cgggtggaca
     1981 gcgcgaacgc gctgatcgcc cgccgcacgt ccggcggata gaagtccgga tagtggcgct
     2041 cgatgtcgcg caggtacggc cgggcgcggt cgaggatcgc gcccggcacc gcgaacccct
     2101 tctggcgcgc cgcggcgagc gcgctggcga cgtagaccga gaggtagggc accgacggat
     2161 agccgcggtc ccagaacgcg accccgccgt cggggttctg cagctgcgac agccgctcga
     2221 gatcgacccg gacgctgtcc gccatcgccg ccgcggacgg catgtccttg gtatggaacg
     2281 cggcgagcac gtcgcgcagc gccgcgatcg ccaggatccg cgacgaccgc tgctcggagc
     2341 actcgaacgg gtagtgcacg aggtagagca tcgcgtcggt cagcgcggcg aggtgggtcg
     2401 acgaagttat gacctcgagg ccgccgaacc ggggcacgac ctggccgggc agcgcgaccg
     2461 gctgggcgat cgcgccgccc ggcgtccggt cgtcgatcac gccgtaggtc gcgaacgcct
     2521 cggtggtc
//
LOCUS       DASVOR010000868         3061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_242427,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000868 DASVOR010000000
VERSION     DASVOR010000868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3061
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242427"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..392)
                     /locus_tag="VF516_17000"
     CDS             complement(<1..392)
                     /locus_tag="VF516_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109433.1"
                     /translation="MNKIAIAFLAATSLASFGCKNKGGAGQAMEMMKKYKADLCACKD
                     HDLDCGKKATDAYQKSMADWAAKNAGEKTDTSVKPDPEMEKLTTEVAECSKRVMTPDM
                     GAAGGAAGGAAAGTPPAGGAAPAGGDTAR"
     gene            complement(616..2562)
                     /locus_tag="VF516_17005"
     CDS             complement(616..2562)
                     /locus_tag="VF516_17005"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX8109434.1"
                     /translation="MKKVERFTTQCLAGLRRAQALRPQLIAAPRTVDQALERYNELLT
                     AMFESNALAGLMSEVHPDEAIRDAARECEQEVEQFRSELMLDREVYDALAAVEVSGAD
                     AETQRFLAHTLRDYRRAGVDQPPEVRTRLKQIDEELTRLNQSFSKNISEDVRTIEVTD
                     PARLAGLPPDFVAAHPADRDGKIRITTDYPDYNPFLTYASDDELRHQLYVAFRSRGDQ
                     HNEAALREILKLRAEKAALLGFTNWADYITADKMIGSGARAAEFIDKVWKLASPRAEQ
                     DYKELLRQLQELAPGATAVADWQKMWLENLVKKQRYEVDAGEVRQYFPHDRVLAGLLE
                     ITAEIFDLTYVPAGDAERWHSSVLVYDVVRGRGAEQTKLGRIYLDMHPRDGKYKHAAQ
                     FPLKDGVRGVQLPEGVLVCNFPAPDPGGPGRPPNAGLMEHDDVVTMFHEFGHLMHHVL
                     GGHQKWISQSGISTEWDFVEAPSQMFEEWACSYDTLKRFARHHETGEVIPRSLVDNMR
                     RADKFGLGTATVQQIFYAAISLNFHRADADALDQLAEVQRLQKRYTPFAYVPGTRFHT
                     SFGHLVGYSAMYYTYLWSLVIAKDLLTPFQARGLMSTEVTYVYRDKVLVPGGSRDAAD
                     LVRDFLGRSYDFAAYEKYLRETAA"
     gene            2592..>3061
                     /gene="miaE"
                     /locus_tag="VF516_17010"
     CDS             2592..>3061
                     /gene="miaE"
                     /locus_tag="VF516_17010"
                     /inference="COORDINATES: protein motif:HMM:NF017939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HEX8109435.1"
                     /translation="MDDVPQLSDALPLCASGPAWLATALGDLDAVHADHLHCERKAAQ
                     SALSLIRSYPERADLVDAMARLAHDETRHVIQVAHLMARRDQPASYDHGDDYAAALRA
                     EIRGREPDRLLDRLLVFAIIEGRSAERLGLLAGALGDPRARELYASLAAAEHRHR"
BASE COUNT          429 a         1057 c         1058 g          517 t
ORIGIN      
        1 cgcgccgtgt caccaccggc cggagccgcg ccacccgcgg gcggcgtacc cgcagccgcg
       61 ccacccgcag cgccgccggc cgcgcccatg tcgggcgtca tcacgcgctt gctgcactcg
      121 gcgacctcgg tggtcagctt ctccatctcc ggatccggct tcacgctggt gtccgtcttc
      181 tcgccggcgt tcttcgcggc ccagtcggcc atcgacttct ggtaggcgtc ggtcgccttc
      241 ttgccgcagt ccagatcgtg gtccttgcag gcgcagagat ccgccttgta cttcttcatc
      301 atctccatcg cctggccggc gccacctttg ttcttgcagc cgaacgaagc gagggacgtg
      361 gcagccagga acgcgatcgc gatcttattc atcggtatct ccggttggtc tcgtgtgcgt
      421 tcgttgtaag ggcatccggc catgcgcgca aagcaaacgt gcatgaactt cgatgaaagc
      481 gatcgcgcac tcgtcgtcga gcgacgacaa tcttatccgg gcgaagacaa ttgcatgcgt
      541 gaacgagcaa cggcgtctcc agccccgaca caatcctgcg acccgatcac aattgtaatc
      601 aggtggtgac gatcgtcagg ccgcggtctc gcgcaggtac ttctcgtagg cggcgaagtc
      661 gtacgacctg ccgaggaagt cgcgcaccag atccgcggcg tcgcggctgc cgccgggcac
      721 gagcaccttg tcgcggtaga cgtaggtgac ctcggtcgac atcagcccgc gcgcctggaa
      781 cggcgtgagc aggtccttcg cgatcaccag cgaccacagg tacgtgtagt acatcgccga
      841 gtagccgacg aggtggccga acgaggtgtg gaaccgcgtg cccggcacgt aggcgaacgg
      901 ggtgtagcgc ttctgcaggc gctggacctc ggcgagctga tcgagcgcgt cggcgtcggc
      961 gcggtggaag ttgagcgaga tcgcggcgta gaagatctgc tgcaccgtcg cggtgcccag
     1021 accgaacttg tcggcgcgcc gcatgttgtc gaccaggctg cgcgggatca cctcgccggt
     1081 ctcgtggtgg cgcgcgaacc gcttcagggt gtcgtagcta cacgcccact cctcgaacat
     1141 ctgcgacggc gcctcgacga agtcccactc ggtggagatc ccgctctggc tgatccactt
     1201 ctggtggccg cccagcacgt ggtgcatgag gtggccgaac tcgtggaaca tcgtcacgac
     1261 gtcgtcgtgc tccatcagcc cggcgttggg cggccggccc gggccgccgg gatccggcgc
     1321 ggggaagttg cagaccagca ccccctcggg gagctggacg ccgcgcacgc cgtccttgag
     1381 cgggaactgc gcggcgtgct tgtacttgcc gtcgcgcggg tgcatgtcga ggtagatccg
     1441 cccgagcttg gtctgctccg cgccccggcc gcgcacgacg tcgtagacca gcacgctcga
     1501 gtgccagcgc tcggcgtcgc cggccggcac gtaggtcagg tcgaagatct cggcggtgat
     1561 ctcgagcagg ccggcgagca cgcgatcgtg cgggaagtac tggcggacct cgccggcgtc
     1621 gacctcgtag cgctgcttct tgaccaggtt ctccagccac atcttctgcc agtcggcgac
     1681 cgcggtcgcg cccggcgcga gctcctggag ctggcgcagc agctccttgt agtcctgctc
     1741 ggcccgcggc gaggcgagct tccacacctt gtcgatgaac tcggccgcgc gcgcgccgct
     1801 gccgatcatc ttgtcggcgg tgatgtagtc ggcccagttg gtgaacccga gcagcgccgc
     1861 cttctcggcg cgcagcttca ggatctcgcg cagcgcggcc tcgttgtgct ggtcgccgcg
     1921 gctgcggaac gcgacgtaca gctggtggcg cagctcgtcg tcgctggcgt aggtgaggaa
     1981 cgggttgtag tcggggtagt cggtggtgat ccggatcttg ccgtcgcggt cggccgggtg
     2041 cgccgcgacg aagtcgggcg gcagcccggc gagccgcgcc gggtcggtga cctcgatcgt
     2101 gcggacgtcc tccgagatgt tcttcgagaa gctctggttg agcctggtca gctcctcgtc
     2161 gatctgcttg agccgggtgc ggacctcggg cggctggtcg accccggcgc gccggtagtc
     2221 gcgcagcgtg tgggcgagga accgctgggt ctcggcgtcg gcgcccgaca cctcgaccgc
     2281 cgcgagcgcg tcgtagacct cgcggtcgag catgagctcg gagcggaact gctcgacctc
     2341 ctgctcgcac tcgcgggccg cgtcgcggat cgcctcgtcg gggtggacct ccgacatcag
     2401 ccctgcgagc gcgttgctct cgaacatcgc ggtcaggagc tcgttgtagc gctccagggc
     2461 ctggtcgacc gtgcgcggcg cggcgatgag ctgggggcgc aacgcctggg cgcggcgtag
     2521 cccggcaaga cactgcgtgg tgaaccgctc gaccttcttc acgtctgtgg agactgtaac
     2581 atcgttccgc gatggacgac gtccctcagc tgtcagacgc gctacccctg tgcgccagcg
     2641 gtccggcctg gctggccacc gcgctcggcg acctcgacgc cgtgcacgcc gaccacctgc
     2701 actgcgagcg caaggccgcc cagtcggcgc tgtcgctgat ccgcagctac cccgagcgcg
     2761 ccgatctggt cgacgcgatg gcgcggctcg cccacgacga gacccgccac gtgatccagg
     2821 tcgcccacct gatggcgcgg cgggatcagc cggcgagcta cgaccacggc gacgattacg
     2881 ccgcggcgct gcgcgccgag atccggggcc gcgagccgga ccgcctgctc gaccgcctgc
     2941 tggtgttcgc gatcatcgag ggccggtcgg ccgagcggct cggcctgctc gccggcgcgc
     3001 tcggcgatcc ccgggcgcgc gagctgtacg ccagcctggc cgccgccgag caccgccacc
     3061 g
//
LOCUS       DASVOR010000869         1718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_9173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000869 DASVOR010000000
VERSION     DASVOR010000869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1718
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..>1718)
                     /locus_tag="VF516_17015"
     CDS             complement(130..>1718)
                     /locus_tag="VF516_17015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8109436.1"
                     /translation="DGSFIRVFQDVRGKYGSEGDYVMTRPLRGPLNNSKVDHSTDTYD
                     TIDWLIKNVPETNGKVGMIGSSYEGFTVLMALVHPHPALKAAIPECPMVDGWRGDDWF
                     HNGAFRQTNFDYMLGQETQRGEGKRVPRAGYDDYDNFRRAGSAGEFARISGLDQLGFW
                     RKLSEHPAYDAWWQGQALDRLLAAEPLTVPTMIVTSLWDQEDIYGGITTYQALEPKDK
                     ANDRLFLVLGPWRHSGMGADGSTLGPLRFDSDPSLAFRRDLARPFFEAYLRDRPTRPS
                     VAPATVYETGTNTWRKLPRWPLACASGCPSPLRPLYLQPGGRTGFPAPTGEGFDEYVS
                     DPAKPVPYLPRPVRFANSDAWHTWLVSDQRSVADRPDVLSYVTEPLTQPLRLAGAPQV
                     NLYAATTGSDADWVVKLIDVYPDEHPPRPELGGYQLPLAMDIFRGRYRDSFEHPTAIP
                     AGAVQRYRFTLPAVDHVVLPGHRLMVQIQSSWFPLYDRNPQTFVDNIFFAKPADYRKA
                     TQRIYHQPGAASAIELPVAP"
BASE COUNT          232 a          538 c          697 g          251 t
ORIGIN      
        1 gtcgacctcg acgccggccg ccccgccgcc gcgaccaggg ccggctcggg cgagcccgac
       61 gacgccgagc ccgatcccga cgagccgggc gagtccggcg gcgcgggcag cggcagcaac
      121 tgactcgcgc tacggcgcga ccggcagctc gatcgcgctg gccgcgccgg gctggtggta
      181 gatccgctgg gtcgccttgc gatagtcggc gggcttggcg aagaagatgt tgtcgacgaa
      241 ggtctgcggg ttgcgatcgt agagcgggaa ccagctcgac tggatctgga ccatgaggcg
      301 gtggccgggc agcacgacgt ggtcgacggc ggggagggtg aagcggtagc gctggacggc
      361 gccggcgggg atcgcggtcg ggtgctcgaa gctgtcgcgg tagcggccgc ggaagatgtc
      421 catcgcgagc gggagctggt agccgccgag ctcgggccgg ggcgggtgct cgtcggggta
      481 gacgtcgatc agcttgacga cccagtccgc gtcgctgccg gtggtcgcgg cgtagaggtt
      541 gacctgcggt gcgccggcga gccggagcgg ctgggtcagc ggctcggtga cgtagctcag
      601 cacgtcgggg cgatcggcga cgctgcgctg gtcggacacc agccaggtgt gccaggcgtc
      661 ggagttggcg aaccggaccg ggcggggcag gtagggcacc ggcttggcgg gatcggagac
      721 gtactcgtcg aagccctcgc cggtcggcgc gggaaagccg gtgcggccgc cgggctggag
      781 gtacagcggg cggagcgggg acgggcagcc gctggcgcag gcgagcggcc agcgcggcag
      841 cttgcgccag gtgttggtgc cggtctcgta gacggtggcc ggcgcgacgc tcggccgggt
      901 cggccggtcg cgcaggtagg cctcgaagaa cgggcgcgcc aggtcgcggc ggaacgccag
      961 cgacggatcg ctgtcgaacc gcagcggacc gagcgtgctg ccgtcggcgc ccatcccgga
     1021 gtgccgccac gggccgagca cgaggaacag ccggtcgttg gccttgtcct tgggctcgag
     1081 cgcctggtag gtcgtgatgc cgccgtagat gtcctcctgg tcccacaggc tggtgacgat
     1141 catcgtcggc acggtgagcg gctcggcggc gagcagcctg tcgagcgcct ggccctgcca
     1201 ccaggcatcg taggccgggt gctcggacag cttgcgccag aacccgagct ggtcgaggcc
     1261 gctgatccgc gcgaactcgc cggccgagcc ggcgcggcgg aagttgtcgt agtcgtcgta
     1321 gccggcgcgc gggacccgct tgccctcgcc gcgctgggtc tcctggccga gcatgtagtc
     1381 gaagttggtc tggcggaacg cgccgttgtg gaaccagtcg tcgccgcgcc agccgtcgac
     1441 catcgggcac tcggggatcg cggccttgag cgccgggtgg ggatggacca gcgccatcag
     1501 cacggtgaag ccctcgtacg acgagccgat catgccgacc ttgccgttgg tctcggggac
     1561 gttcttgatc agccagtcga tcgtgtcgta ggtgtcggtc gagtggtcga ccttgctgtt
     1621 gttgagcggg ccgcgcagcg ggcgggtcat gacgtagtcg ccctcggagc cgtacttgcc
     1681 gcggacgtcc tggaagaccc ggatgaagct gccgtcgg
//
LOCUS       DASVOR010000870         1935 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_125468,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000870 DASVOR010000000
VERSION     DASVOR010000870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1935
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125468"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1869)
                     /locus_tag="VF516_17020"
     CDS             complement(<1..1869)
                     /locus_tag="VF516_17020"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX8109437.1"
                     /translation="MKKPRSKKVTAVKAAPAGARTPVAVEPAPRVSTHPAPPAAPAPS
                     TRPAAAQAYTLDGPVPPEAEGLPRKVGALLGIGQRALGIKSFRPGQAQAFEHLLAGED
                     LLAVMPTGSGKSLLYQLTSLVVPGVTVVVSPLIALIKDQLDKMVAKGVSACKIDSTLT
                     VKQRREVDELVRSPGGKLLLTTPERMADPEFRVFLREACGGVGVSRFVVDEAHCVSTW
                     GHDFRPAYLSLRKALEDVGRPPVLATTATAPPHVRDDILFQLGMEGATTVTTTFDRPN
                     LHYEVIVFGDEDEKMKTLVTLLKKLPRPGIVYCATVKKVEELYEALTRWGIPVGRYHG
                     RMTKRERDDSQQKFMDSSELVMVATNAFGLGVDKANIRNVLHYHVPGSVEAYAQEAGR
                     GGRDGKPARCVLLFSPDDVAIQEYFLSGSYPTKRQVRQVYEALEAFTEVHRAGASAGG
                     GPAAKLAEESPPTVGNIALSSGVGQQRTRTVLSLLKDEHFVIEQEGGLYLLADPPPEP
                     ELIADRARQYEARRIADRQRLDALLAYVKAPGCRNQAILEYLGEPDPPRCGRCDNCLR
                     SREAALAASTEATRLGASLTRQLDEDVGEAKPRREIRARVVRIDQPAAAPAGATAAA"
BASE COUNT          249 a          688 c          704 g          294 t
ORIGIN      
        1 ggccgccgcc gtggcacccg cgggcgccgc ggccggctga tcgatccgca ccacgcgcgc
       61 gcggatctca cgccgcggct tggcctcgcc gacatcctcg tcgagctgac gggtcagcga
      121 tgcgccgagc cgggtcgcct cggtcgacgc ggcgagcgcg gcctcgcgcg accgcaggca
      181 gttgtcgcac cgcccgcagc gcggcggatc gggctcgccg aggtactcga ggatcgcctg
      241 gttgcggcag cccggcgcct tgacgtaggc gagcagcgcg tcgagccgct ggcgatcggc
      301 gatccggcgg gcctcgtact ggcgcgcgcg atcggcgatc agctcgggct cgggcggcgg
      361 atcggcgagc aggtacagcc cgccctcctg ctcgatcacg aagtgctcgt ccttgagcag
      421 cgacagcacg gtgcgggtgc gctgctggcc gacgcccgac gacagcgcga tgttgccgac
      481 cgtcggcggc gactcctcgg cgagcttggc cgccggaccc ccgccggcgc ttgcgccggc
      541 acgatgcacc tcggtgaacg cctccagcgc ctcgtagacc tggcggacct ggcgcttggt
      601 cgggtagctg cccgacagga agtactcctg gatcgcgacg tcgtcgggcg agaacagcag
      661 cacgcagcgc gccggcttgc cgtcgcgccc gccgcggccg gcctcctggg cgtaggcctc
      721 gaccgagccc ggcacgtggt agtgcaggac gttgcggatg ttggccttgt cgacgccgag
      781 gccgaacgcg ttggtcgcga ccatcaccag ctcggacgag tccatgaact tctgctggct
      841 gtcgtcgcgc tcgcgcttgg tcatccggcc gtggtagcgc ccgaccggga tgccccagcg
      901 ggtcagcgcc tcgtacagct cctcgacctt cttgaccgtc gcgcagtaca cgatgcccgg
      961 acgcggcagc ttcttgagca gcgtcacgag cgtcttcatc ttctcgtcct cgtcgccgaa
     1021 gacgatcacc tcgtagtgca ggttggggcg atcgaaggtc gtggtgaccg tggtcgcgcc
     1081 ctccatcccg agctggaaca ggatgtcgtc gcggacatgc ggcggcgcgg tcgccgtggt
     1141 cgcgagcacc ggcgggcggc cgacgtcctc gagcgccttg cgcagcgaca ggtacgccgg
     1201 gcggaagtcg tggccccagg tcgacacgca gtgcgcctcg tcgaccacga accgcgacac
     1261 gccgacgccg ccgcacgcct cgcgcaggaa cacccggaac tccgggtccg ccatccgctc
     1321 gggcgtggtc aagagcagct tgccgcccgg cgagcgcacc agctcatcga cctcgcggcg
     1381 ctgcttgacg gtcagcgtcg agtcgatctt gcacgcgctg acgcccttgg cgaccatctt
     1441 gtcgagctgg tccttgatca gcgcgatcag cgggctgacc acgaccgtga ccccgggcac
     1501 gaccagcgag gtcagctggt acagcagcga cttgccgctg ccggtcggca tcaccgcgag
     1561 caggtcctcg ccggcgagca ggtgctcgaa cgcctgggcc tggccggggc ggaacgactt
     1621 gatcccgagc gcgcgctggc cgatgccgag cagcgcgccg accttgcggg gcagcccctc
     1681 ggcctcgggc gggaccggac cgtccagggt gtaggcctgc gccgcggcgg ggcgcgtcga
     1741 gggagccggg gctgccgggg gcgccggatg tgtggagacc cgtggcgctg gttcgaccgc
     1801 gaccggggtt cgggcaccgg caggtgcggc cttgaccgcc gtgactttct tgctgcgagg
     1861 tttcttcaca gaagccggct cgaccggggt tttttttgcc ggctcggcgc gccgggtggt
     1921 ccgcttggcc ttcgt
//
LOCUS       DASVOR010000871         2020 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_125475,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000871 DASVOR010000000
VERSION     DASVOR010000871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2020
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125475"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF516_17025"
     CDS             <1..214
                     /locus_tag="VF516_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109438.1"
                     /translation="RERRWRRGVRHARAPAVGRHTVVGVRGGMAIECLPGRLRLRGGS
                     YWDQKRCSHVEVAMIPLSRYWQIALA"
     gene            complement(377..1228)
                     /locus_tag="VF516_17030"
     CDS             complement(377..1228)
                     /locus_tag="VF516_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109439.1"
                     /translation="MPDHAVSRQLAARAVATIGGNPFYLQLLGEQLAGLAPPLDEGAL
                     KEALSRLVFHRTGRLAMFFEAELTRVVGRSAQALAILEQLARAPCRPADLQAALRLSS
                     SSAVNYLARLGDVIRRRDDDRYELTDPVMALWLKWRAPGGAAVPMTVVGDDGERDVAR
                     ALADLGFELVYQSKASRGAFDLLAIRAGVMVGVQVKRSALPLHFTAAAWKRMEAEARR
                     LGWLWIVAAASPDGVVTFLRPGARPPPERGRPVRVGGHRQPPGVGRRRLHTTSPPVTR
                     SSRSSRR"
     gene            complement(1318..>2020)
                     /locus_tag="VF516_17035"
     CDS             complement(1318..>2020)
                     /locus_tag="VF516_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013799987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8109440.1"
                     /translation="EALPRHLGERWRERMGTDILDGIGSTEMLHIFLSNRPGDIHYGT
                     TGRPVPGYDLELRGDDDRPIPEDGPGSGAAEGALWVRGPTSCTGYWNERTRSLATFHG
                     PWTRTGDRYSRGADGTWTYGGRGDDMLKVSGIWVSPFEVESALAAHPAVLEAAVVGHE
                     DADGLTKPKAFVVLRDPAAGGPALADELKSFVKTHLAPFKYPRWVEVVGELPKTATGK
                     IQRFKLRGGAQTRRT"
BASE COUNT          279 a          703 c          743 g          295 t
ORIGIN      
        1 ccgcgagcgg cgctggcgtc gaggcgttcg gcatgcacga gctccagccg tcggtcgcca
       61 caccgtggtc ggcgtgcgcg gcggcatggc catcgagtgc ctgccgggcc ggctgcggct
      121 gcgcggcggc tcgtactggg accagaagcg gtgctcacat gtcgaagtgg caatgatacc
      181 gctatcccgg tactggcaga tcgcccttgc ttgaagcatt gccgcttttc gcgggtcgtg
      241 gcgatccctg aaggccgcgc cggacgtccc gcgcgcgctg gagctcatag aatcggcggg
      301 cgcaggactc gagctcgccg cggtgtcacc tcgacctcgt catcaggcgc acgcaccacc
      361 ggcacttcgt ttgcgttcaa cggcgactgg acctcgacga cctcgtcacc ggcggcgacg
      421 tggtgtggag gcgtcgtcga cccacgccag gaggttgacg atggccgccg acgcggacag
      481 gccgacccct ttccggcggc ggccgcgcgc cgggtcggag gaaggtgacg acgccgtcgg
      541 ggcttgccgc cgccacgatc cagagccagc cgagtcttcg agcttcagcc tccatccgtt
      601 tccaggctgc ggcggtgaag tgcagcggca gcgccgagcg cttcacctgg acgcccacca
      661 tcaccccggc gcggatcgcg aggaggtcga acgcgccacg cgaggccttc gactggtaca
      721 cgagctcgaa gccgaggtcg gccagcgcgc gggcgacgtc gcgctcaccg tcgtcgccca
      781 ccaccgtcat cggcaccgcg gcgcctcccg gggcgcgcca cttcagccag agtgccatca
      841 ccgggtcggt cagctcgtag cggtcgtcat cgcgccggcg gatgacatcg ccgaggcgcg
      901 ccaggtagtt caccgcggac gacgaggaga gccgcaaggc cgcctggaga tcggcggggc
      961 gacatggtgc gcgtgcgagc tgctcgagga tcgccagcgc ttgtgccgag cggccgacga
     1021 cgcgggtgag ctccgcttcg aagaacatcg cgagccgtcc cgtacggtgg aagaccagcc
     1081 gcgacagcgc ttctttcagc gcgccttcgt cgagtggcgg cgccagcccc gcgagctgct
     1141 ccccgaggag ctggaggtag aacgggttgc cgccgatggt cgctaccgcg cgggcggcga
     1201 gctgccgcga caccgcgtgg tcgggcaccg ctccctcgac gaggagggca atggcgtccg
     1261 cctccgcgaa acgttacaaa tgtcggtccc ggctacgtcg tgcggaagcg gccggggtca
     1321 cgtgcggcgg gtctgggcgc cgccgcgcag cttgaaccgc tggatcttgc cggtcgcggt
     1381 cttgggcagc tcgccgacga cctcgaccca gcgcgggtac ttgaacggcg cgaggtgggt
     1441 cttgacgaag ctcttgagct cgtcggccag ggccgggccg ccggcggccg gatcgcgcag
     1501 cacgacgaac gccttgggct tggtcaggcc gtcggcgtcc tcgtggccga ccaccgcggc
     1561 ctcgagcacg gccgggtgag cggcgagcgc cgactcgacc tcgaatggcg acacccagat
     1621 cccgctgacc ttgagcatgt cgtcgccgcg gccgccgtag gtccaggtgc cgtcggcgcc
     1681 gcggctgtag cggtcgccgg tgcgggtcca cgggccgtgg aacgtcgcga ggctgcgcgt
     1741 gcgttcgttc cagtagccgg tgcagctggt cgggccgcgc acccacagcg cgccctcggc
     1801 ggccccggag ccggggccat cttcagggat gggtcgatcg tcgtcaccgc gcagctcgag
     1861 atcgtagccc ggcaccggcc ggccggtcgt gccgtagtgg atgtcgcccg gccggttgct
     1921 gaggaagatg tgcagcatct cggtcgagcc gatgccatcg aggatgtcgg tccccatccg
     1981 ctcgcgccag cgctcgccga ggtggcgggg cagggcctcg
//
LOCUS       DASVOR010000872         2600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67015,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000872 DASVOR010000000
VERSION     DASVOR010000872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2600
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67015"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2028)
                     /locus_tag="VF516_17040"
     CDS             complement(<1..2028)
                     /locus_tag="VF516_17040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109441.1"
                     /translation="MKSESADLVVTQTASPTEAPSDDDRGRRVPPVTGDDPSAATQIG
                     NPTRYRILGEHGRGARGRVSRAHDRSLGRDIAIKELISRGDLDEVRFLREALITARLE
                     HPGIVPVYEAGRWPDGTPFYAMKLVAGRPLSEILAERTTVDERIPLLHHVIAVADAIA
                     YAHGRNIIHRDLKPANIIVGDFGETVVIDWGLAKDLTVPEAATASDGPPHLPQDDGLT
                     ATGSVMGTPAYMPPEQRRGVPVDQRADVFAIGAMLWELCALQRTPPSSAAQRRRILRR
                     AGIDRDLIAIIDKAVDPDPACRYPDAGALASDLKAFKAGARISARDYSLLAMLVHWTR
                     RHRALAVSVAAAMTLVVVGDIYYVRNIAAERDRADQERDRARLSEASVLIEHDPTRAR
                     SLLASLTARSPQYALLMSRANQHAAARVILEPAAIAAVFRAPGAAELVLLTPDGEFYR
                     VDPQTGARQLIDHEVGRAVTYHAGDWLYTRKPFDTRAMSIATPSTHDALNARGLTRVS
                     RLVSLRDSVYALDAQGDLYRLGREDPVLVRRDVHQVAGDGNWLLICSQDGTLEGLRDG
                     TVMLRERCPHRLSPGTMVVRGDDYAAVTDTGMLIASRAGHVLKLPTAISGEFELALSS
                     AGILAVADYAQGGSAWFIRPDASAIARAPVHPGPVAVAAEGRYAAWGYYDGT"
     gene            2217..>2600
                     /locus_tag="VF516_17045"
     CDS             2217..>2600
                     /locus_tag="VF516_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109442.1"
                     /translation="MFAPTPRSSSRAPDGLPLVMHVHRAVDVRGPVLRALDASAPLDR
                     DLERTLADLERTTRSCTITRRMVRVGRRAYYETAGSVAGHLGATLLARMLGAVSPLIL
                     PDDVAAAREPSRFENSVLLSVQELDA"
BASE COUNT          371 a          938 c          911 g          380 t
ORIGIN      
        1 cgtgccgtca tagtatcccc aggcggcata tcgcccctcg gctgccacgg cgaccggccc
       61 tggatgcacg ggagcccgcg cgatcgcgga cgcgtcgggg cggatgaacc aggcgctgcc
      121 tccctgtgcg tagtcagcga ctgccaggat tccggcactc gagagcgcca gctcgaattc
      181 ccccgagatc gcggtcggca gcttgaggac atggcctgcg cgagaggcga tcagcatgcc
      241 ggtgtcggtc acggcggcgt agtcgtcgcc gcgaaccacc atcgttcccg ggctcagtct
      301 gtggggacat cgctcgcgga gcatgaccgt gccgtcgcgg aggccctcga gcgtcccgtc
      361 ctgcgagcag atcaggagcc agttgccatc cccggcgacc tgatgaacgt cgcgccgtac
      421 gagcacggga tcctcgcggc ccaaccggta caggtcaccc tgcgcatcga gcgcatagac
      481 cgaatcccgc aacgacacga gcctggacac ccgggtcaat ccacgtgcgt tgagcgcatc
      541 atgcgtcgac ggtgtcgcga tgctcatggc cctggtgtcg aatggctttc gggtgtagag
      601 ccagtcgccc gcgtggtagg tcaccgcccg gccgacctcg tggtcgatca gctggcgggc
      661 gccggtctgc ggatccacgc gatagaactc accgtcgggc gtcagcagca caagctcggc
      721 ggcaccgggc gcccggaaga ccgccgcgat cgctgccggc tccaggatga cgcgggcggc
      781 ggcgtgctgg ttggcacggc tcatcagcag cgcatactgc ggagatcgcg ccgtcaacga
      841 cgcgagcagg ctccgggcgc gcgtcggatc gtgctcgatg aggaccgacg cctccgacag
      901 ccgggcgcga tcgcgctcct gatccgcgcg gtcgcgctcg gccgcgatgt tgcggacgta
      961 gtagatgtcg ccgacgacca cgagggtcat cgccgccgcg accgacaccg cgagcgcgcg
     1021 atgccggcgg gtccagtgca cgagcatggc cagcagcgag taatcacgcg ccgagatccg
     1081 ggcgcccgcc ttgaatgcct tgaggtcgga cgccagcgca ccggcgtcgg ggtagcggca
     1141 cgcgggatcc gggtcgactg ccttgtcgat gatcgcgatc aggtcgcggt cgatgcccgc
     1201 gcggcgcagg atccgccggc gctgggcagc gctgctcggc ggcgtccgct gcagggcgca
     1261 cagctcccac agcatggcgc cgatcgcgaa cacgtcggcg cgctggtcga ccggcacgcc
     1321 gcgccgctgc tccggcggca tgtaggccgg cgtgcccatg acgctgccgg tggccgtgag
     1381 cccatcgtcc tgcgggagat gaggcggccc gtcgctggcc gtcgcggcct cgggcacggt
     1441 gagatccttg gccagccccc agtcgatcac gacggtttcc ccgaagtcgc cgacgatgat
     1501 gttggccggc ttgaggtcgc ggtggatgat gttgcggccg tgggcgtagg cgatcgcgtc
     1561 ggccacggcg atcacgtggt ggagcagggg gatgcgctcg tcgaccgtcg tgcgctcggc
     1621 gaggatctcg ctcagcgggc gaccggcgac cagcttcatg gcatagaacg gggtgccgtc
     1681 gggccagcgg cccgcctcgt acaccgggac gatgcccgga tgctcgaggc gcgcggtgat
     1741 caacgcttcg cgcaagaagc gcacctcgtc caggtcgccg cgcgagatca gctccttgat
     1801 cgcgatgtcg cgaccgaggc tgcgatcgtg ggcgcgcgac acgcggcctc tggcaccgcg
     1861 gccgtgctcg ccgaggatcc ggtagcgcgt ggggttgccg atctgggtcg ccgccgacgg
     1921 gtcgtcgcct gtaaccggcg ggacgcgtcg acctcggtca tcgtccgacg gcgcctccgt
     1981 gggcgaggcg gtctgggtca ccaccaggtc tgccgactcg gacttcatcc agcgtacccc
     2041 gcagtctacc ggaagtcgcg ccctcggcga ccggtcggcc atgagcgcgc gtcccaagct
     2101 tttcccggcc ggcgtgtcca accgggacat cgcaccggag ccaccgatga tcgaacccac
     2161 ccaccggatc cgcgaatccc accgcaccat cgagatcacc cgccccgccg atctcgatgt
     2221 tcgccccgac gccgcgttcg agctcgcgcg cgcccgacgg cctgccgctc gtgatgcacg
     2281 tccatcgcgc ggtcgacgtc cgtggcccgg tgctgcgcgc cctcgacgcc agcgcgccgc
     2341 tcgatcgcga tctggagcgc acgctggccg atctggagcg caccacccgg tcgtgcacca
     2401 tcacccgccg catggtgcgc gtcggccgcc gcgcgtacta cgagaccgca ggctcggtcg
     2461 ccggccacct cggcgccacg ctgctcgccc gcatgctcgg cgcggtcagc ccgctgatcc
     2521 tgcccgacga cgtcgccgcc gcgcgcgagc cgtcgcggtt cgagaacagc gtcctgctct
     2581 cggtccagga gctcgacgcc
//
LOCUS       DASVOR010000873         2111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_9195,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000873 DASVOR010000000
VERSION     DASVOR010000873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9195"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..801
                     /locus_tag="VF516_17050"
     CDS             <1..801
                     /locus_tag="VF516_17050"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HEX8109443.1"
                     /translation="GRAVAVVAGMLGEAYPEVAREAGAIARVITEEAGRFEHALEHGL
                     RVLERRAALDGKAAFDLLQTHGLPFEVTRELAEAAGKPVDEDGFRREQEAHRTRSRPA
                     GGETFRGGLADHSAEVVGYHTVTHLLLAALRAVLGPHVIQRGSNLTHERLRLDFSHGD
                     KLGPEELARVAARVDGWLARDLVVERATMTEAEARGSGAIGAFGEKYGEIVSVYGIVD
                     RATGEVVSRELCGGPHVASTAELRGRRLRIVREQAIASGIRRIKAVLG"
     gene            1022..>2111
                     /locus_tag="VF516_17055"
     CDS             1022..>2111
                     /locus_tag="VF516_17055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109444.1"
                     /translation="MGRADGTDPAMVDTPVPLDGDGHELIGMIIDGRYRLDATLGRGG
                     MGLVYRAAHVGLRRQVAVKILHPSLAASPEVRNRFEREALAVGKIDHPNCVSVYDVGR
                     LPDGALYLAMELLEGRALADVLEQDGQIAPGRVLHILAGVLRGLAHIHAAKLIHRDIK
                     PENIFLIRQGQDEDFAKILDFGIAKPMAASDLDDGVKLTQAGMAFGTPIYMAPEQALG
                     NPMDGRADLYAAAVIAYEMLCGQPPFYSEDKLEVMAMHTARPVPPMRQRLIKGGRPVP
                     SSIERLVVRGLTKKPSDRYATAEEFLAEVERALRTPDGGVTDVVLERRGDTGSQPLVV
                     AGGEVRITGETDFAAGAAQTVVDAQASIA"
BASE COUNT          299 a          669 c          877 g          266 t
ORIGIN      
        1 gggcgcgcgg tggcggtggt ggcgggcatg ctgggcgagg cgtaccccga ggtcgcgcgc
       61 gaagcggggg cgatcgcgcg ggtgatcacc gaggaggccg gccggttcga gcacgcgctg
      121 gagcacgggc tgcgggtgct cgagcggcgc gcggcgctcg acggcaaggc ggcgttcgac
      181 ctgctgcaga cccatgggct gccgttcgag gtcacgcgcg agctggcgga ggcggcgggc
      241 aagccggtcg acgaggacgg gtttcgccgc gagcaggagg cgcaccgcac gcggtcgcgg
      301 ccggccgggg gcgagacgtt tcgcggcggg ctcgcggacc actcggcgga ggtcgtcggc
      361 taccacacgg tcacgcacct gttgctggcg gcgctgcgcg cggtgctcgg gccccacgtg
      421 atccagcgcg gctcgaacct cacgcacgag cggctgcggc tggacttctc gcacggcgac
      481 aagctcggcc ccgaggagct ggcgcgggtc gcggcgcggg tcgacggctg gctggcgcgc
      541 gacctggtgg tcgagcgcgc gacgatgacc gaggccgagg cgcgggggag cggcgcgatc
      601 ggggcgttcg gcgagaagta cggcgagatc gtctcggtgt atgggatcgt ggatcgcgcg
      661 accggcgagg tggtgagccg cgagctctgc ggcggaccgc acgtggcgtc gaccgcggag
      721 ctgcgcggcc ggcggctgcg gatcgtccgc gagcaggcga tcgcgtcggg gatccgccgg
      781 atcaaggcag tgctgggctg agcgctgcga atctggatga cacgggcccg cgacgacggc
      841 cttgcaactc ctttcagcgt ctggcggctg ggcaatttgt gcgccgcagc acgcgacggc
      901 gtccgggaga taacgcagcg ccggtgcgca cagggccggc gtgaggcgcc gcgggacgtc
      961 ggggaaaacc gacgacgcga tgggcgcgat cggggcgaga tggcacaatg agggccgcag
     1021 catgggcaga gccgacggca ccgatccggc gatggtggac acgcctgtcc cgctggacgg
     1081 cgatggccac gagctgatcg gcatgatcat cgacgggcgc taccggctcg acgcgacgct
     1141 cgggcgcggc gggatggggc tggtgtaccg cgcggcgcac gtcgggctgc gccggcaggt
     1201 cgcggtcaag atcctgcatc cgtcgctggc ggcgtcgccg gaggtgcgca accggttcga
     1261 gcgcgaggcg ctggcggtcg gcaagatcga tcacccgaac tgcgtgagcg tgtacgacgt
     1321 cgggcggctg ccggacggcg cgctgtacct ggcgatggag ctgctggagg gccgcgcgct
     1381 ggccgacgtg ctggagcagg acggccagat cgcgccgggc cgggtgctgc acatcctcgc
     1441 cggggtgctg cgcgggctgg cgcacatcca cgccgccaag ctgatccacc gcgacatcaa
     1501 gccggagaac atcttcctga tccggcaggg ccaggacgag gactttgcca agatcctgga
     1561 cttcgggatc gccaagccga tggcggcgtc ggatctcgac gacggggtca agctgacgca
     1621 ggccgggatg gcgttcggca cgccgatcta catggcgccc gagcaggcgc tgggcaaccc
     1681 gatggacggc cgcgccgacc tgtacgccgc ggcggtgatc gcgtacgaga tgctgtgcgg
     1741 gcagccgccg ttctactccg aggacaagct cgaggtgatg gcgatgcaca ccgcgcggcc
     1801 ggtgccgccg atgcggcagc ggctgatcaa gggcgggcgg ccggtgccgt cgtcgatcga
     1861 gcggctggtg gtgcgcgggc tgaccaagaa gccgagcgac cgctacgcga cggcggagga
     1921 gttcctggcc gaggtcgagc gcgcgctgcg cacgccggac ggcggggtga ccgacgtcgt
     1981 gctcgagcgg cgcggcgaca ccggatcgca gccgctggtg gtcgctggcg gcgaggtccg
     2041 gatcaccggg gagaccgact ttgcggcggg cgccgcccag acggtggtcg acgcgcaggc
     2101 ctcgatcgcc g
//
LOCUS       DASVOR010000874         3902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_184351,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000874 DASVOR010000000
VERSION     DASVOR010000874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184351"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VF516_17060"
     CDS             <1..674
                     /locus_tag="VF516_17060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8109445.1"
                     /translation="REAFADRYRVVTFDHRGTGDSDKPEAPPYTTRGFADDAIAVLDH
                     LGIERAHAYGMSMGGRIGQWLGIAYPGRLGALVLGCTTPGNAHGVRRPAHLDPVLLSG
                     DPERILPYLASLAWAAANRGFLAERNAWLLAHPVPPHARQLHYQASEGHDAWDQLPQI
                     TTPTLLIHGSDDEMNVVANARLLAERIPGAELHIIPGARHVYYWDHGEEANRVVGEFL
                     ARHPL"
     gene            complement(700..2211)
                     /locus_tag="VF516_17065"
     CDS             complement(700..2211)
                     /locus_tag="VF516_17065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109446.1"
                     /translation="MYRGAAFDREYDADAMIGDVKQLIATDPETGARALGELALLYVS
                     TETPHAYCRGFALSLWDDAAMQAALPRKWLDSVENYLVDIIAAYPRIAGRIPRAFDRN
                     GCVGPLVSARDVPALLAHVEQVLDAMPPGDRVRYRPLWEVLRVAAARGLGYWEGTDID
                     VIQTHESWLAPVRRPRVVTAPSPLTSAHARPLAMSGDRMLVGEHFVLHELDTASFPPG
                     AVTHDDMQVTAAAFTPWGTEFVRMVTDREARPFKFSYFELPDRRPLAIDPGFAVGLAR
                     PTRDGVLLFPLPTTQRRTGARPVVLRLPNVLEPLALPEAADAERIECEAVAFGDGSLL
                     VVWDGVPYRWDGVAPPESLGGTLEAPDDPCAAVALSDGSIVGGFGRKLVRIDRDGQRA
                     AVLPLDNVVGVARGPEDTLIISEGDNPEGDALKLWWPATRELTHVQPKLLELDERPMF
                     VYFDANAQLVVAARPRMWHALAWSELDAMRRVSEDEFIARRAALVERPSRGPG"
     gene            complement(2411..3502)
                     /locus_tag="VF516_17070"
     CDS             complement(2411..3502)
                     /locus_tag="VF516_17070"
                     /inference="COORDINATES: protein motif:HMM:NF016103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HEX8109447.1"
                     /translation="MSASHRRHAVSVLALAVLGCGGRYGGAPVERPQPAARGMEAAAL
                     PYQIVDARTGKSVDEPAFWGRLAAAHAVCVGEEHPNPHHHWMQLHVVRELIKRLPPGT
                     RLALAMEMFQRPFQGVLDDFAARRIDAAQLRSRSGYEDRWGYDYAFYGPTIDAALGAG
                     AQLLAANAAKELTKKVVHHGLESLTPEERARLPELKLDDRIHRAWFDALMEDMGGSAA
                     HSHSKSKDATDDGDQAAGKPSPRHGGDAGDEMPSADRVYTVQVIWDETMADTSARWLA
                     ANPGGHLILLAGNGHCHDSAIINRMKRRGIADAVSVRGVIDDGEGSVGEALARPINDF
                     LVVLQMPPDVQRAARADDKDDDRADDKAR"
     gene            3526..>3902
                     /gene="truD"
                     /locus_tag="VF516_17075"
     CDS             3526..>3902
                     /gene="truD"
                     /locus_tag="VF516_17075"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HEX8109448.1"
                     /translation="MTLPYLTAALAGTGGTLRTTDDDFVVDEELPYAPSGAGDHVFVR
                     IEKRGTTTVDAARALARALGVRDRDIGIAGMKDRHAVTRQWLSLPPPVAPEQALAVEL
                     PGVRVLEAHRHAHKLRTGHVRANR"
BASE COUNT          540 a         1409 c         1428 g          525 t
ORIGIN      
        1 tgcgcgaggc gttcgccgac cgctaccggg tggtgacgtt cgaccaccgc ggcacgggcg
       61 acagcgacaa gccggaggcg ccgccgtaca cgacgcgcgg gttcgccgac gatgcgatcg
      121 cggtgctcga ccacctgggg atcgagcggg cgcacgcgta cggcatgtcg atgggcggcc
      181 ggatcggcca gtggctgggc atcgcgtatc ccgggaggct cggggcgctg gtgctgggct
      241 gcacgacgcc gggcaacgcg cacggggtgc ggcggccggc gcacctcgac ccggtgctgc
      301 tgagcggcga cccggagcgg atcctgccgt acctggcgtc gctggcgtgg gcggcggcga
      361 accgcggctt cctggccgag cgcaatgcgt ggctgctggc gcacccggtg ccgccgcacg
      421 cgcgccagct gcactaccag gcgagcgagg ggcacgacgc gtgggatcag ctaccgcaga
      481 tcaccacgcc gacgctgctg atccacggca gcgacgacga gatgaatgtg gtggccaacg
      541 cgcggctgct cgccgagcgc atccccggcg ccgagctgca catcatcccc ggcgcgcggc
      601 acgtgtacta ctgggaccac ggcgaggagg cgaaccgcgt ggtgggcgag ttcctggcgc
      661 gccacccgct gtagccagcg cgcgcggccg cggcgccggt catcccggcc cgcgcgacgg
      721 gcgttcgacc agggctgcgc gccgggcgat gaactcgtcc tcggagacgc gccgcatcgc
      781 atcgagctcg ctccacgcca gcgcgtgcca catccggggt cgcgccgcga cgaccagctg
      841 ggcgttcgcg tcgaagtaga cgaacatggg ccgctcgtcg agctccagca gcttgggctg
      901 gacgtgcgtg agctctcgcg tcgccggcca ccacagcttc aaggcatccc cttccgggtt
      961 atcgccctcg ctgatgatca gcgtgtcctc cggaccgcgc gccactccca cgacgttgtc
     1021 gagcggcagc acggccgcgc gctggccgtc gcgatcgatg cgaacgagct tgcgaccgaa
     1081 cccgccgacg atcgatccat cggacagcgc aacggcggcg catggatcgt cgggcgcctc
     1141 cagcgtaccg ccgagcgact ccggcggcgc gacgccatcc cagcgatatg gcacgccatc
     1201 ccacacgacg agcagcgagc cgtcgccgaa cgcgaccgcc tcgcactcga tgcgctccgc
     1261 atccgccgcc tcgggcagcg ccagcggttc gagcacgttg ggcaggcgca gcacgaccgg
     1321 gcgcgcgccg gtgcggcgct gcgtcgtggg cagcgggaac agcagcacgc catcgcgggt
     1381 gggccgcgcg aggccgaccg cgaaccccgg atcgatggcg agcggccggc gatccggcaa
     1441 ctcgaagtag ctgaacttga agggccgcgc ctcgcgatcc gtgaccatcc ggacgaactc
     1501 cgtgccccac ggggtgaacg ccgcggcggt cacctgcatg tcgtcgtgcg tgaccgcgcc
     1561 gggcgggaag ctcgccgtgt ccagctcgtg gagcacgaag tgctcgccga cgagcatccg
     1621 gtccccggac atcgcgagcg gccgcgcgtg cgcgctggtc agcggactcg gcgcggtcac
     1681 cacccgaggc cggcgcaccg gcgcgagcca gctctcgtgg gtctggatga cgtcgatgtc
     1741 ggtgccctcc cagtagccca ggccgcgcgc cgcggcgacg cgcagcacct cccacagcgg
     1801 ccggtaccgt actcgatcgc ccggcggcat cgcgtccagc acctgctcga cgtgcgccag
     1861 cagcgcaggc acgtcgcgcg ccgacaccag cggtccgacg cagccgttgc gatcgaacgc
     1921 acgcgggatg cgcccggcga tccgggggta cgccgcgatg atgtccacga ggtagttctc
     1981 gacgctgtcg agccactttc tcggcagcgc cgcctgcatc gcggcgtcgt cccacagcga
     2041 cagcgcaaac ccgcggcagt acgcgtgggg cgtctcggtc gacacgtaga gcagcgcgag
     2101 ctcgccgagc gcgcgggccc cggtctcggg atccgtggcg atcagctgct tgacgtcgcc
     2161 gatcatcgcg tcggcgtcat actcgcgatc gaacgcagcg ccgcgataca tgccgcgcag
     2221 gaacctcgcc gagaacctcg cgagcgaagc gcggtcgacg acgtggaggg tgcaatccca
     2281 gcccacacta ccccctcgcc ctcgcgcaac ggtcggaacg cggcgatccg gcgatcgccg
     2341 gatccagcct ggatcgcatg ctcgcgtcgt cgtcgcagcg catcgaccgc tccaccaggt
     2401 ctcggcgcgg ttacctggcc ttgtcgtcgg ccctgtcgtc atccttgtca tcggccctgg
     2461 cggcgcgctg gacgtcgggc ggcatctgca gcacgacgag gaagtcgttg atcggccggg
     2521 cgagggcctc gccgaccgag ccctcgccgt cgtcgatcac gccgcgcacc gagacggcgt
     2581 cggcgatgcc gcggcgcttc atgcggttga tgatcgcgga gtcgtggcag tggccgttgc
     2641 cggcgagcag gatcaggtgg ccgcccgggt tcgcggccag ccagcgcgcg ctggtgtcgg
     2701 ccatggtctc gtcccagatc acctggacgg tgtagacccg gtcggcgctc ggcatctcgt
     2761 cgccggcgtc gccgccgtgc cgggggctcg gtttgcccgc ggcctggtcg ccgtcgtcgg
     2821 tggcgtcctt ggacttgctg tgcgagtgcg ccgcgctgcc gcccatgtcc tccatcagcg
     2881 cgtcgaacca cgcgcggtgg atgcggtcgt cgagcttgag ctcgggcagc cgggcccgct
     2941 cctcgggggt cagcgactcg aggccgtggt gcacgacctt cttggtcagc tccttggcgg
     3001 cgttggcggc cagcagctgg gcgccggcgc cgagcgcggc gtcgatcgtc gggccgtaga
     3061 acgcgtagtc gtagccccag cggtcctcgt agcccgaccg cgagcgcagc tgcgcggcgt
     3121 cgatccggcg ggcggcgaag tcgtcgagca cgccctggaa cgggcgctgg aacatctcca
     3181 tcgccagcgc gagcctggtg ccgggcggca gccgcttgat cagctcgcgc acgacgtgga
     3241 gctgcatcca gtggtggtgc gggttggggt gctcctcgcc gacgcacacc gcgtgcgccg
     3301 cggcgagccg gccccagaac gcgggctcgt cgaccgactt gccggtccgc gcatcgacga
     3361 tctggtacgg cagcgccgcg gcctccatgc cgcgcgctgc gggctgggga cgctcgaccg
     3421 gggcaccgcc gtagcgaccg ccgcaaccaa ggacggccag cgcgaggacc gaaaccgcgt
     3481 ggcggcggtg cgatgctgac atgctccgat ggtacaccgt gacgtgtgac cttgccgtac
     3541 ctcactgcag cgctcgccgg gaccggtggg accctgcgca cgacggacga cgacttcgtg
     3601 gtggatgagg agctcccgta cgcgccctcg ggtgcgggag atcacgtgtt cgtgcggatc
     3661 gagaagcgcg gcacgacgac ggtcgatgcg gcgcgggcgc tggcgcgcgc gctcggcgtg
     3721 cgcgatcgcg acatcggcat cgccgggatg aaggaccgcc acgcggtgac ccggcagtgg
     3781 ctgtcgctgc cgccgccggt ggcgcccgag caggcgctcg cggtcgagct gccgggcgtg
     3841 cgggtgctgg aggcccaccg ccacgcgcac aagctgcgca ccgggcacgt ccgcgccaac
     3901 cg
//
LOCUS       DASVOR010000875         1687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_213407,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000875 DASVOR010000000
VERSION     DASVOR010000875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213407"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VF516_17080"
     CDS             <1..677
                     /locus_tag="VF516_17080"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEX8109449.1"
                     /translation="AIQPELAQYDPTATGHTFLYTLEQWVDDTASWQPACQVDTDGRN
                     VAIPLAAIWDEHGDRSVSSAMFTFGCTTGVIAKCYRWGYRPWVTDYGDLTTMHWTCTR
                     MARADYCGNGVPHTHDGTLINMWDTLPPPGPIQSHGGLLPPLGMLFEAGWNTGGAVCL
                     SRARWPLDKNGLFVNLCPGRLVPPGLLGATVCDTVADALAQDPNARMFNESYLNVSGT
                     VTQVLR"
     gene            complement(662..>1687)
                     /locus_tag="VF516_17085"
     CDS             complement(662..>1687)
                     /locus_tag="VF516_17085"
                     /inference="COORDINATES: protein motif:HMM:NF014213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M18 family aminopeptidase"
                     /protein_id="HEX8109450.1"
                     /translation="HTDSPNLRIKPNAEYRSEGYAQVGVEVYGGALLNSWLDRDLGVA
                     GRVIVRDDRGALDSRLVCLERPLLRVPQLAIHLDREVNDKGLVLNRQDHLSPVLGLAA
                     DGASLGALIAEAAGVAADRVVAGDLMLFDVTPPVRGGAAGELIFSARLDNLAMCHTAV
                     TAIAQITRPSAIPVIALFDHEEVGSESAAGAASAVLPRILERLAGSREAFHQACARST
                     CVSADMAHAVHPNYPGRHEPRHRPQLNGGPVIKTNTQQRYATSAATAAMFAELCRAEE
                     VAVQHYVHRTDLPCGTTIGPITATRLGIATVDVGNPMLSMHSARELAGARDPELMTRV
                     LTRYLST"
BASE COUNT          231 a          617 c          596 g          243 t
ORIGIN      
        1 ccgcgatcca gcccgagctc gcgcagtacg atccgaccgc gaccggccac accttcctgt
       61 acacgctgga gcagtgggtc gacgacaccg cgtcgtggca gccggcgtgt caggtcgaca
      121 ccgacggccg caacgtcgcg atcccgctgg cggcgatctg ggacgagcac ggcgaccgca
      181 gcgtgtcgag cgcgatgttc acgttcggct gcaccaccgg cgtgatcgcg aagtgctatc
      241 gctggggcta ccggccgtgg gtcaccgatt acggcgacct gacgacgatg cactggacct
      301 gcacccggat ggcgcgcgcc gactactgcg gcaacggcgt gccgcacacc cacgacggca
      361 ccctgatcaa catgtgggac accctgccgc cgcctgggcc gatccagagc cacggtggcc
      421 tgctgccgcc gctcggcatg ctgttcgagg ccggctggaa caccggcggc gcggtgtgcc
      481 tgagccgcgc gcgctggccg ctcgacaaaa acggcctgtt cgtcaatctg tgccccggcc
      541 gtctggtccc acccggcctg ctcggcgcga cggtgtgcga tacggtcgcc gatgccctgg
      601 cccaggaccc gaacgccagg atgttcaacg agtcgtacct caacgtgtcc ggcaccgtga
      661 ctcaggtgct caggtagcgc gtcagcacgc gggtcatgag ctcggggtcg cgggcgccgg
      721 cgagctcgcg cgccgagtgc atgctgagca tcgggttgcc gacgtcgacc gtcgcgatgc
      781 cgagccgcgt cgcggtgatc ggcccgatcg tcgtgccgca cggcaggtcg gtccggtgca
      841 cgtagtgctg gaccgccacc tcctcggcgc ggcacagctc ggcgaacatc gccgcggtcg
      901 ccgcgctggt cgcgtagcgc tgctgggtgt tggtcttgat caccggcccg ccgttgagct
      961 gcgggcggtg gcgcggctcg tggcggcccg ggtagttcgg gtgcacggcg tgcgccatgt
     1021 cggccgagac gcaggtcgag cgcgcgcacg cctgatggaa cgcctcgcgg ctgccggcga
     1081 gcctctcgag gatccgcggc agcaccgcgc tcgccgcgcc cgccgcgctc tcgctgccga
     1141 cctcctcgtg atcgaacagc gcgatcaccg ggatcgccga cggccgcgtg atctgcgcga
     1201 tcgccgtgac cgcggtgtgg cacatcgcga ggttgtcgag ccgcgccgag aagatcagct
     1261 cgcccgccgc gccgccccgc accggcggcg tcacgtcgaa cagcatcaga tcgccggcga
     1321 ccacgcggtc cgcggcgacc cccgccgcct cggcgatcag cgcgcccagc gacgcaccgt
     1381 cggcggcgag cccgagcacc ggcgagaggt gatcctggcg gttgagcacc agccccttgt
     1441 cgttgacctc gcgatcgagg tggatcgcga gttgcggcac gcggagcagc ggtcgctcca
     1501 ggcacaccag ccggctgtcc agcgcgccgc ggtcgtcgcg cacgatcacg cggccggcga
     1561 cgccgaggtc gcgatcgagc cacgagttga gcagcgcgcc gccgtagacc tcgacgccga
     1621 cctgcgcgta gccctcgctc ctgtactccg cgttcggctt gatccgcagg ttcgggctgt
     1681 cggtgtg
//
LOCUS       DASVOR010000876         5202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417302,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000876 DASVOR010000000
VERSION     DASVOR010000876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417302"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..916
                     /gene="argC"
                     /locus_tag="VF516_17090"
     CDS             <1..916
                     /gene="argC"
                     /locus_tag="VF516_17090"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX8109451.1"
                     /translation="VHPNLDGATDLVFEDLAPAEAARGCDVALLALPHKVTAAKVPEL
                     IAAGVKIVDMSGDFRLRDAAAYEKYYGARHPHPELLGTFVYGLPELHRAEIKRAKDVA
                     SPGCFATAMELALLPLARAGLLDGATVHVTGITGSSGSGIAPGAGTHHPSRAGNLKTY
                     KPLEHQHVPEVVQALGDAGARDVQLRFVPVSAPLTRGIFATCFLELAADVDAARLAAL
                     FDESYAREPFTRRPRKRLPEVVAVAGSNFIEVGFAVGPASGGKRTVTCFSAIDNLIKG
                     GAGQAIQNMNLMLGCDERASLEDVGNWP"
     gene            913..1737
                     /gene="argB"
                     /locus_tag="VF516_17095"
     CDS             913..1737
                     /gene="argB"
                     /locus_tag="VF516_17095"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX8109452.1"
                     /translation="MTRPTIIKIGGEVIGSGEAAVLAGDVKALVGGGARIAIVHGGGP
                     QATDLQKRLGIPTVQVAGRRVTDPATLDVMKMVLAGKLNVDLCATLLAAGLTPVGLHG
                     ASGHLIRAVRRPPKPYPSAGPDPVDLGLVGDVTGFHLPLLDTLWAAGYLPVIACLGAD
                     ATGGVYNINADLVGNQLAAALRAERLFLVTSTPGVLRDVNDPASRLTRLSCAEARRAI
                     GDGVVTGGMIAKLEEAMAVIDQGVGAIHILGKLAAGDLVRAVREPGSVGTTLVADA"
     gene            1830..2534
                     /locus_tag="VF516_17100"
     CDS             1830..2534
                     /locus_tag="VF516_17100"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8109453.1"
                     /translation="MRTSVVGIAMLGWLAACAPPAAAPRSPEPAACDQGKGPSCVELA
                     ERYDHGTGVEQSTARALELYLQACNHDVARGCRAAGDITTRGEGVPRDEARGLALREK
                     ACQLDDAIACNTMGVAYSEGKDGAAAVDHARARTHYEKACKLHNGVGCFNLGNVFRIG
                     EGTPVDLRRALAEFQRGCDLDAAQGCTEAAIIYCEGQAVPRDLDKGVALFEKGCRLGS
                     AVACKNRDTFRDAQKP"
     gene            2691..3356
                     /locus_tag="VF516_17105"
     CDS             2691..3356
                     /locus_tag="VF516_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109454.1"
                     /translation="MACAVVAGVGCPAMHGGSFEATGFRSTYGYEIPYQHGTQSLLPA
                     DWALVNYERTSGGGWARKTSDRYVTKYELDDDDNGGDGRSGSFVEWPTYAVRYEHRVH
                     AGAIWLRDIDEVHVAPFARTSRVQLVLLRAPQDEVYEPGNGHLPTHRYPVVLLAGYSN
                     MPADFAKGLDDFHGLLQKIAIGNKTGLTLELAPSAPPPPVGTPPAATTPADTVPPPPP
                     AQP"
     gene            3399..4001
                     /locus_tag="VF516_17110"
     CDS             3399..4001
                     /locus_tag="VF516_17110"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8109455.1"
                     /translation="MEPEDVPPPVDFHDPAHARKWTDETPRKRPWRPSFFAEFCTALR
                     GARLRILELGSGPGHLAREILTRCDVAAYLALDFSSAMHDLAREHLDELASRVTFVTR
                     DFRTPDWTAGLSAFDAIVTMQAVHETRHKRRALPLLAQARTILRPGGQLLYCDHYFED
                     GKKVGLMLDRAEQPEVLRAAGYSDVELLRDEGGMALYAAR"
     gene            4080..4265
                     /locus_tag="VF516_17115"
     CDS             4080..4265
                     /locus_tag="VF516_17115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109456.1"
                     /translation="MSYQKVCKRRGRVANQASAVSVPSKYLGGISVGVHLLTVDQFPV
                     VAWKGKKITGVLGVAMP"
     gene            complement(4341..>5202)
                     /locus_tag="VF516_17120"
     CDS             complement(4341..>5202)
                     /locus_tag="VF516_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109457.1"
                     /translation="SSGLAARGLLALHAILAGDLVIARGVLPALASEPAHRALAGTIA
                     GMLARADAIAGVSPLDAGDLRGWHFVTTGGLLLHRARHGLDHGMNGRYAFVQDGHADC
                     LEGLRRLAAVIAAWRRPIECVIAPDERASRILAIAAAEVLGCTVGSLDAPAGLVVVYD
                     LDRLGPADRAALRIADPARPLYAHSAVWTEERPVAADLVVRLCQFYRAPWDARTGIDP
                     ATGAPCAIPADDTSEAELAARITGAPLAADALDDLAVVLALAGVVHPAATGERSRAWA
                     GSPVPSLRFT"
BASE COUNT          753 a         1968 c         1803 g          678 t
ORIGIN      
        1 cgtgcacccc aacctcgatg gcgccaccga cctggtgttc gaggacctcg cacccgccga
       61 ggccgcccgc ggctgcgacg tcgccctgct cgccctgccc cacaaggtca ccgccgccaa
      121 ggtccccgag ctgatcgccg cgggggtcaa gatcgtcgac atgtccggcg acttccggct
      181 gcgcgacgcc gccgcgtacg agaagtacta cggtgccagg cacccccacc ccgagctgct
      241 cgggaccttc gtctacggcc tgcccgagct ccaccgcgcc gagatcaagc gcgcgaagga
      301 cgtcgcgtct cccggctgct tcgccaccgc gatggagctc gccctgctgc cgctcgcccg
      361 cgccggcctg ctcgacggcg ccaccgtcca cgtcaccggc atcaccggct cgtcgggctc
      421 gggcatcgcc cccggcgccg gcacccacca cccgagccgc gccggcaacc tcaagaccta
      481 caagccgctc gagcaccagc acgtccccga ggtcgtccag gccctgggcg acgccggcgc
      541 cagggacgtc cagctgcggt tcgtcccggt gtccgcgccg ctgacccgcg gcatcttcgc
      601 gacctgcttc ctcgagctcg ccgccgacgt cgacgccgcc cggctcgccg ccctgttcga
      661 cgagtcctac gcccgcgagc cgttcacgcg ccggcccagg aagcggctgc ccgaggtcgt
      721 cgcggtcgcc ggctccaact tcatcgaggt cggcttcgcc gtcggcccgg cgtccggcgg
      781 caagcgcacc gtcacctgct tctcggcgat cgacaacctg atcaagggcg gcgccggcca
      841 ggcgatccag aacatgaacc tgatgctcgg ctgcgacgag cgcgccagcc tcgaagacgt
      901 cgggaactgg ccgtgacccg ccccacgatc atcaagatcg gcggcgaggt gatcggctcc
      961 ggcgaggccg ccgtgctcgc cggcgacgtc aaggcgctgg tcggcggcgg tgcccggatc
     1021 gccatcgtcc acggcggcgg cccccaggcc accgacctgc agaagcggct cggcatcccg
     1081 accgtccagg tcgccggccg ccgcgtcacc gatccggcca cgctcgacgt catgaagatg
     1141 gtcctggccg gcaagctcaa cgtcgacctg tgcgccaccc tgctcgccgc cggcctcacg
     1201 cccgtcggcc tgcacggcgc gtccggccac ctgatccgcg ccgtccgccg cccgccgaaa
     1261 ccctacccca gcgccggccc cgatccggtc gacctcggcc tggtcggcga cgtcaccggc
     1321 ttccacctgc cgctgctcga caccctgtgg gcggccggct acctcccggt catcgcctgc
     1381 ctgggcgccg acgccaccgg cggcgtctac aacatcaacg ccgatctcgt cggcaaccag
     1441 ctcgccgcgg cgctgcgcgc cgagcgcctg ttcctcgtca cctccacccc cggcgtgctg
     1501 cgcgacgtca atgacccggc ctcgcggctc acccgcctgt cgtgcgccga ggcccgccgc
     1561 gcgatcggcg acggcgtcgt caccggcggc atgatcgcca agctcgagga ggcgatggcc
     1621 gtgatcgacc agggcgtcgg cgcgatccac atcctcggca agctcgccgc cggcgacctg
     1681 gtccgcgccg tccgcgagcc cggctcggtc ggcaccacgc tggtcgccga cgcctgattg
     1741 ccatcgcacg ccgccggcga tcgccatcgc accgtcggtc cgtggcgatc cgggtcgatc
     1801 gcatcgggca ggcgtcgtac gatgccgcga tgcggacgtc ggtcgtcggg atcgcaatgc
     1861 tcggatggct cgcggcgtgc gctccccccg cggccgcgcc caggtcgccg gagccggccg
     1921 cgtgcgatca gggcaagggg ccgagctgcg tcgagctggc ggagcgctac gaccacggca
     1981 ccggcgtcga gcagagcacc gccagggcgc tggagctcta tctccaggcc tgcaaccacg
     2041 acgtcgcgcg cggctgccgt gccgcgggcg acatcaccac gcgcggcgag ggggtccccc
     2101 gcgacgaggc ccgcggcctg gcgctgcgcg agaaggcgtg ccagctcgac gacgcgatcg
     2161 cgtgcaacac catgggagtc gcctactccg aaggcaagga cggcgcagcc gcggtcgacc
     2221 acgctcgcgc gcggacgcac tacgagaagg cgtgcaagct ccacaacggc gtcggctgct
     2281 tcaacctcgg caacgtgttc cgcatcggcg agggcacccc ggtcgatctg cggcgcgcgc
     2341 tcgccgagtt ccagcgcggg tgcgatctcg acgcggcgca gggatgcacc gaggcggcga
     2401 tcatctactg tgagggccag gccgtgcccc gcgacctcga caagggggtg gcgctgttcg
     2461 agaagggctg ccgcctcggc agcgcggtcg cgtgcaagaa ccgcgatacg ttccgcgacg
     2521 cgcagaagcc gtgagccgat tgcgatgctc gggaatcggt gggtgccggc gccgcgcttg
     2581 atcgcagcct gggcattgca gtagggtcgc gcgtcatgac gaaatctcgg cgcgaggcgt
     2641 gtggtcgcgg gcgaggggat cgaccgggga cgctggcaag ctttgtcgcg gtggcgtgcg
     2701 ccgtcgtggc gggcgtgggc tgtccggcga tgcacggcgg gagcttcgag gccaccggct
     2761 tccggtcgac gtacggttac gagatcccgt accagcacgg gacccagagc ctcttaccgg
     2821 ccgactgggc gctcgtgaac tacgagcgga cgtcgggtgg gggatgggca cgcaagacgt
     2881 cggaccgcta tgtcacgaag tacgagctcg acgacgatga caacggcggc gacggccggt
     2941 ccgggtcatt cgtcgagtgg cccacgtatg ccgtgcgcta tgagcaccgc gtccatgccg
     3001 gcgcgatctg gctgcgcgac atcgacgagg tccacgtggc tcctttcgcc cgcacgtcgc
     3061 gcgtccagct cgtgctgctg cgcgcgcccc aggacgaggt ctacgagccg ggcaatggcc
     3121 acctgcccac gcatcgctac ccggtggtcc tgctcgccgg gtactcgaac atgccggcgg
     3181 actttgcgaa ggggctcgac gactttcacg ggctgctgca gaagatcgcg atcgggaaca
     3241 agaccgggct caccctcgag ctcgccccgt ccgcgccgcc acctcccgtc ggcacgccgc
     3301 cggcagccac cacgccggcc gacaccgtgc caccccctcc cccggcgcag ccatagggct
     3361 tcgcgccagt agctgccgtc ccgtggcatg gtgaccgtgt ggagcccgag gacgtaccgc
     3421 cccctgtcga ctttcacgat ccggcacacg cgcggaagtg gaccgacgag acgcctcgca
     3481 agcgaccgtg gcgcccgagc ttcttcgccg agttctgcac ggcgctgcgc ggcgcgcggc
     3541 tgcgcatcct cgagctcggc tccggccccg gccacctggc gcgcgagatc ctcacgcgct
     3601 gcgacgtcgc cgcgtatctc gccctcgact tctccagcgc gatgcacgac ctcgcgcgcg
     3661 agcacctcga cgagctcgcc tcgcgcgtca cgttcgtcac gcgcgacttc cgcacgcccg
     3721 actggaccgc aggcctctcc gcattcgacg cgatcgtcac gatgcaggcg gtgcacgaga
     3781 cccgccacaa gcgccgcgcg ctgccgctgc tcgcccaggc gcgcacgatc ctgcggccag
     3841 gcggccagct gctctattgc gaccactatt tcgaggacgg caagaaggta gggctgatgc
     3901 tcgaccgcgc cgagcaaccc gaggtattgc gcgccgcggg ctattccgac gtcgagctac
     3961 tgcgcgacga aggcggaatg gcgctctacg ccgcccggtg aacgacggcg gcgcactgcg
     4021 gtggacacct ggagtgggtc gagacccacc gcgtcgcgca ggtcacgccg aatggctcgg
     4081 tgagctacca gaaggtctgc aagcggcgcg gccgggtcgc caaccaggcg agcgcggtca
     4141 gcgtcccgtc gaagtacctc ggtggcatct cggtcggcgt ccacctgctc accgtggacc
     4201 agttcccggt ggtggcctgg aagggcaaga agatcaccgg agtgctcggg gtcgcgatgc
     4261 cgtgacacgg cggaatagga tcagccgctg gacgcgagca aacacacgca gcccgatgcg
     4321 tggcggcctg cggtgagcca tcaggtgaag cgcaggctgg gcacaggcga tccagcccac
     4381 gcccgcgagc gctcgccggt ggcggccggg tgcacgacgc cggccagcgc gagaaccacg
     4441 gcgagatcgt cgagcgcgtc ggcggcgagc ggcgcaccgg tgatccgcgc ggcgagctcg
     4501 gcctcgcttg tgtcgtcggc cgggatcgcg cacggcgcac ccgtggcggg atcgatgccg
     4561 gtccgcgcgt cccacggcgc gcggtaaaac tggcacagtc gcacgaccag atcggccgcg
     4621 accgggcgct cctcggtcca caccgcgctg tgcgcgtaga gcgggcgcgc cggatcggcg
     4681 atgcggagcg ccgcgcggtc cgccgggccg agacgatcga ggtcgtagac cacgacaaga
     4741 cccgccggcg cgtcgagcga gccgaccgtg cagcccagga cctcggccgc agcgatcgcg
     4801 aggatccggc tggcgcgctc gtcgggcgcg atcacgcact cgatcggccg ccgccatgcg
     4861 gcgatcacgg cggcgagccg gcgcaggccc tcgaggcagt ccgcgtgacc gtcctggacg
     4921 aacgcgtagc ggccgttcat gccgtgatcg aggccatggc gcgcacggtg cagcagcagg
     4981 cctcctgtgg tgacgaagtg ccagccgcgc agatcaccgg cgtcgagcgg cgacacgccg
     5041 gcgatcgcgt cggcgcgcgc cagcatgccc gcgatcgtcc cggcgagcgc gcgatgcgcc
     5101 ggctcgctgg ccagcgcggg gagcaccccg cgcgcgatca cgagatcgcc ggcgaggatc
     5161 gcgtggagcg caagcaggcc acgcgcggcg agccccgagc tc
//
LOCUS       DASVOR010000877         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_213427,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000877 DASVOR010000000
VERSION     DASVOR010000877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213427"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..2141)
                     /locus_tag="VF516_17125"
     CDS             complement(15..2141)
                     /locus_tag="VF516_17125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX8109458.1"
                     /translation="MAPNTAVMFVLSGTCLALSIPRVRSRARRWLAHALALLSGLLAA
                     LTLAAYVFGVDLGLARLILRASERTAPETALAFMLLASALLTLDLTRRRALIPVELLA
                     MGALVLGALALGGYAYGAPQFYAPDVHRHPVGMAVHTSAGLIVLAIGVLCAAGNAGLM
                     ATVANPRLAGRVVRRVLAIVLVVPVVGFFAAWAQNTGLYDPPGATVITTVGGLVVAVL
                     SALRLGHWLDRTDAERRRSDDRLRALRDWGEVFDHASFGAWLGSADGHLIRVNEAFAR
                     MHGCTRGAAEGRPLAEFFPEDRREELATSLCIVHDRGHHRWESENLRTDGSVFPVVID
                     SGAVYGRDGEVRYRATYVQDITEDKRAEAEGARLAAIVRSTDDAIVSTTLDGIVVDWN
                     HAAERAFGYAADEMIGRSIELIVPEDRRAERRALIAKTMAGEPMVGVEAIRVCKDGQQ
                     IPVALTMSALRDSSGRASGISVIERDISVLKQLQREHEEWSAVVAHDLRQPASTIRFA
                     AEMLRGLDGEPKQRVLVRVDHACGRLEQMIEDLLDVSRIEARRLVVRPSSLRVAPLLE
                     EILDSAPDLARRCTVQVEDGAVEVWADAGRVSQVLSNLLSNADKYSYRGTRIDVHVAR
                     AEGMVEITVTNEGPGIEPDEIPLLFSRFARTRAARRGQAPGLGLGLYISSGLVEAQGG
                     RMWVESTPGQRTSFHFTLPGAPPGKP"
     gene            complement(2452..>2831)
                     /locus_tag="VF516_17130"
     CDS             complement(2452..>2831)
                     /locus_tag="VF516_17130"
                     /inference="COORDINATES: protein motif:HMM:NF021523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2252 family protein"
                     /protein_id="HEX8109459.1"
                     /translation="SHQGKRIAEGQHRMQTWTDPLLGWTAIGGVPFYVRQLSDHKASI
                     DPADLRRSPLVEYARVCGETFAKAHGRTGDAAVLYGYAGQSDKLDRAIAELALLGADQ
                     VTRDWEILAGAIGRGDLEAKELD"
BASE COUNT          429 a         1035 c          981 g          386 t
ORIGIN      
        1 ctgatcggcg cgcttcaggg ctttcccggc ggcgccccgg ggagcgtgaa gtggaacgac
       61 gttcgttgcc ccggggtgct ctcgacccac atccgtccgc cctgcgcctc gacgagcccg
      121 ctggagatgt agagcccgag cccgagtccc ggcgcctggc cgcgccgcgc cgcgcgggtc
      181 cgcgcgaacc gcgagaacag cagcgggatc tcatcgggct cgatgcccgg gccctcgttc
      241 gtcacggtga tctccaccat gccctccgcg cgcgcaacgt gaacgtcgat ccgcgttccg
      301 cggtagctgt acttgtcggc gttcgacagc aggttggaca gcacctggct gacccgccca
      361 gcgtcggccc acacctcgac cgcgccgtcc tcgacctgca cggtacagcg gcgtgcgaga
      421 tccggcgcgg aatcgaggat ctcttcgagg agcggtgcga cgcgaagact gctcggccgc
      481 acgaccagcc ggcgcgcctc gatccgcgac acgtcgagca ggtcctcgat catctgctcg
      541 agccgcccgc aggcgtggtc gacgcgcacg agcactctct gcttcggctc gccgtcgagg
      601 ccccggagca tctcggccgc gaaccggatg gtcgacgcgg gctggcggag atcgtgcgcg
      661 acgaccgccg accactcctc atgctcgcgc tggagctgct tgagcacgct gatatcgcgt
      721 tcgatcaccg agatgccgct ggcgcggccc gaggagtccc ggagcgcgga catcgtgagc
      781 gcgaccggga tctgctgccc atccttgcac acgcggatgg cctcgactcc gaccatcggc
      841 tcaccggcca tcgtcttcgc gatcagcgcg cgccgttcgg cccggcgatc ctcgggaacg
      901 atgagctcga tcgaccggcc gatcatctcg tcggccgcat agccgaaggc gcgctcggcg
      961 gcgtggttcc agtcgaccac gatcccgtcg agcgtcgtcg acacgatggc gtcatccgtc
     1021 gagcgcacga tcgccgccag ccgggcgcct tcggcctcgg cacgcttgtc ctccgtgatg
     1081 tcctggacgt acgtggcgcg gtagcggacc tcgccatccc gcccgtagac cgcgccggag
     1141 tcgatgacga ccgggaacac gctgccgtcc gtgcggaggt tctccgattc ccagcggtgg
     1201 tggccacggt catggacgat gcagaggctc gtcgcgagct cctcgcgccg gtcctcgggg
     1261 aagaactcgg cgagcggcct gccctcggcc gcgccccgcg tgcagccatg catccgggcg
     1321 aacgcttcgt tcacccggat caggtgaccg tccgcggacc cgagccaggc gccaaagctc
     1381 gcgtggtcga agacctcccc ccagtcgcgc agtgcgcgca accgatcgtc gctccggcgc
     1441 cgctccgcgt cggtgcggtc gagccagtgg ccgagccgga gcgcggagag caccgcgacg
     1501 accaagccgc cgaccgtggt gatgacggtg gcccccggcg gatcgtacag cccggtattc
     1561 tgcgcccacg ctgcgaagaa gccgacgacg gggacgacca gcacgatcgc gagcacgcgg
     1621 cggacgacgc ggcctgcgag gcgcgggttg gccaccgtcg ccatcagccc ggcgttgccg
     1681 gccgcgcaca gcacgccgat cgcgaggacg atgagcccgg ccgaggtgtg gaccgccata
     1741 cccacggggt ggcgatgtac gtcgggcgcg tagaactgcg gagcgccgta cgcgtagccc
     1801 ccgagcgcga gcgcccccaa gaccagcgcc cccatggcca gcagctcgac ggggatcagc
     1861 gcgcgccgcc gggtgaggtc gagcgtgagc agcgccgagg cgaggagcat gaacgcgagc
     1921 gccgtctcgg gcgcggtccg ttccgacgcg cgaaggatca gcctcgccag cccgagatcg
     1981 acaccgaaga cgtacgccgc cagcgtgagc gccgcgagca gcccggagag cagggcgagc
     2041 gcgtgcgcta accaccgcct cgcgcgcgaa cgcactcgcg ggatcgacag cgcgaggcag
     2101 gtgccgctca gcacgaacat gaccgcggtg ttcggtgcca tcggccaatc gccgagggga
     2161 ttcacgatcc cctcgagacg atccgcccat cccacaagca cgacggcgcc gagcaggatg
     2221 gcaaccgcag cggccacgcg cccgccgatc gcgaacccac ggtcacggcg agcctcgtcc
     2281 gtgccctgat gctcccctgc ttcggtcatc gtctgccctg atacgccggg agggaagcaa
     2341 gatggatact aggtcgcgac gacccagccg gatgcggacg aggcggtcgc cctctggacg
     2401 ccatcctcct cgtcttccgg agaggatacg gccgaggccc agcgcgccgg ttcagtcgag
     2461 ctctttggcc tcgagatcgc cgcggccgat cgcgccggcc aggatctccc agtcgcgggt
     2521 gacctgatcg gcgccgagca gggcgagctc ggcgatcgcg cggtcgagct tgtcggactg
     2581 cccggcgtag ccgtagagca ccgcggcgtc gccggtccgg ccgtgcgcct tggcgaacgt
     2641 ctcgccgcac acccgcgcgt actcgaccag cggcgagcgc ctgaggtcgg cgggatcgat
     2701 cgacgccttg tggtcggaca gctggcggac gtagaacggc acgccgccga tcgccgtcca
     2761 gccgagcagc ggatcggtcc aggtctgcat ccggtgctgg ccctcggcga tccgcttgcc
     2821 ctggtggctg g
//
LOCUS       DASVOR010000878         1678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_38032,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000878 DASVOR010000000
VERSION     DASVOR010000878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1678
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38032"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..510
                     /locus_tag="VF516_17135"
     CDS             <1..510
                     /locus_tag="VF516_17135"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEX8109460.1"
                     /translation="KPAADGAARGLVKDFSRRPALYSQDGTRHATCCIVIRLKRAREP
                     AARGDGHRVLVDRLWPRGLRKADAHIDAWRKDVAPSTELRRWFSHDPRRFPEFRERYR
                     QELRSEPARAALAELARRAARGTVTLVYAARDELHNNAVVLAQELSRRVTRVRAQRAV
                     VSRARTRPA"
     gene            complement(482..844)
                     /locus_tag="VF516_17140"
     CDS             complement(482..844)
                     /locus_tag="VF516_17140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109461.1"
                     /translation="MTKPTPITESAVFDLAAIDRELRSEDAYLRDGHTARTLVREPAI
                     RIVLVVIKAGARIAEHRAQEITSIQALTGAVRLALSDRSLDLAAGRVLVVAPDLRHHV
                     EAVADSALLLTLAWCERG"
     gene            1044..>1678
                     /locus_tag="VF516_17145"
     CDS             1044..>1678
                     /locus_tag="VF516_17145"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8109462.1"
                     /translation="MSSIPSAVPSSLNVPVSEAAVAARAAPASLASRAARGALWTVLS
                     SIGGRAIGVLGTLAMTRFLHPDQIGEVSDAMIIVMTASWVTVWGFGQYSVVKGRGAEA
                     DEVTWYATVGYAGLGAISLGLVAVLGGRLTPFLGAPHAAVYIPGLAFALYLRRLAAMP
                     ERVLTRDMKFGASGVSSAAGEASYTATALGFAVLGFGGWAIVIGNIVQSIV"
BASE COUNT          210 a          590 c          620 g          258 t
ORIGIN      
        1 aagcccgccg cggacggcgc cgcacgcggg ctcgtcaaag atttctcgcg gcggccggcg
       61 ttgtacagcc aggatggcac gcgccatgcg acctgctgca tcgtgatccg actcaagcga
      121 gcccgcgaac cggcggcgcg cggcgacggt catcgcgtgc tcgtcgatcg gctgtggccg
      181 cgcggactgc gcaaggccga cgcgcacatc gatgcatggc gcaaggacgt ggcgccgagc
      241 accgagcttc gccgctggtt cagccacgac ccgcggcgat ttcccgagtt tcgcgagcgc
      301 taccggcaag agctccgctc cgagccggcc cgtgcggcgc tcgccgagct cgcgcgtcgc
      361 gccgctcgcg gcacggtcac gctggtgtac gcggcgcgcg atgagctgca caacaacgcg
      421 gtcgtgctcg cgcaggagct gtcgcgccgg gtcactcgcg tgcgcgcgca gcgggcggtg
      481 gtcagccgcg ctcgcaccag gccagcgtga gcagcagggc gctgtcggcg accgcctcga
      541 cgtggtggcg gagatcgggc gccaccacca gcacgcgtcc agcggccaga tcgagcgacc
      601 ggtcgctcag cgcgagccgc acggcgccgg tgagcgcctg gatcgacgtg atctcctgcg
      661 cgcgatgctc tgcgatccgc gcgccggcct tgatcaccac cagcacgatc cggatcgccg
      721 gctcgcggac cagcgtgcgc gcggtgtggc cgtcgcgcag gtaggcgtcc tcggatcgca
      781 gctcgcgatc gatcgcggcg agatcgaaca cggcactttc ggtgatcggt gtgggcttgg
      841 tcatctcgac tcctctgcct ttggaacagt ctgttcgacc aatccgcgtg aggccatcgc
      901 ggcggcgcgg ggatcgccgt gccgccgcgc tacagccgtg gcgcgcgagc gcgtgcgaaa
      961 ccgctcgccg cgatctaact cgcacttgac cgcctccgtc ggtggcgggg cgcggtttgc
     1021 atgtgaggct gcgaactgtc gccatgtcgt cgatcccatc cgcggtcccg agctccctga
     1081 acgtcccggt gtccgaggcg gcggtcgcgg cgcgcgctgc gccggcgtcg ctcgcgagcc
     1141 gcgctgcccg cggtgcgctg tggaccgtgc tgtcgagtat cggcggccgc gcgatcggcg
     1201 tgctcggcac gctggcgatg acgcggttcc tccaccccga ccagatcggc gaggtgtcgg
     1261 atgcgatgat catcgtgatg accgcgagct gggtgacggt ctgggggttc ggacagtact
     1321 cggtcgtcaa gggccgcggc gccgaggccg acgaggtcac ctggtacgcg accgtgggct
     1381 acgccgggct cggcgcgatc tcgctcgggc tggtcgcggt gctcggcggc cggctgacgc
     1441 cgttcctcgg cgcgccgcac gccgcggtgt acatccccgg cctggcgttc gcgctgtacc
     1501 tccggcggct cgcggcgatg ccggagcgcg tgctgacccg cgacatgaag ttcggcgcgt
     1561 cgggggtgtc gagcgccgcc ggcgaggcga gctacaccgc gaccgcgctc ggcttcgccg
     1621 tgctcggctt cggcggctgg gcgatcgtga tcggcaacat cgtgcagtcg atcgtcat
//
LOCUS       DASVOR010000879         2140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417334,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000879 DASVOR010000000
VERSION     DASVOR010000879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2140
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417334"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VF516_17150"
     CDS             complement(<1..510)
                     /locus_tag="VF516_17150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109463.1"
                     /translation="MLEVRARRERGGALNHSEVLNASFVSDEGQVIRLLLEAAPRPSQ
                     GGHTRFYVSDLTTPNSGHATGSGTDRASALLDGVRQILESGSAYGRGSVSVRIDGRTF
                     SRRIEASQAALLMETIENAAMAISIAAVAAAPFTGGASLAILLPVGVIGAVPSAYRLA
                     TRAENGTLRF"
     gene            complement(540..1721)
                     /locus_tag="VF516_17155"
     CDS             complement(540..1721)
                     /locus_tag="VF516_17155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109464.1"
                     /translation="MPPPWLRYDLFVQEVARFGPQLRDYVTALERFRASQAEPDRAAA
                     VAALRALLDAINVPARVLEQIRGDAALVAHDAYRALWPVPQGQPPHLAAPDRAPDPRI
                     AELFLMFARTQPAQSTAALQATDARRELLDRFRRFVVNAATTPGATGDLVIQDAPGRA
                     NAQPHPSTMSSYPVLQPPLFEAALRTDHRFVMQLQFPSVFDAFARTSYFWERVRVPDS
                     QIGGVTDAATARGERPTRGEVGSAHSDRANRYARADIGRAVQDITSNLGEAGVGALEI
                     AAAAAIFRYIGTGLRLAFEIATRPDNEQPIVFPEEGVYLVRCRAVPIPGDNDEVVRAP
                     SVAYLPVFATSGQRMAEQQTEQAQAMNDQAITRLAEIERLLAGPGQVSDRAALEAERG
                     R"
     gene            complement(1643..>2140)
                     /locus_tag="VF516_17160"
     CDS             complement(1643..>2140)
                     /locus_tag="VF516_17160"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8109465.1"
                     /translation="LFGAHAFTLGDDLFFSAGAYAPGDAQGRWVLAHETEHVLQARRG
                     AAANVVRRTPGSVAGHAPTTGHAPTTDTPVAMSFALTGITFIVPENITTRLLGWLDAQ
                     HLAVDVTAAQRELISLGMATTQAWHGFPGATPPSWQRRCRRRGCATTCSSRRSRASAR
                     SCVTT"
BASE COUNT          278 a          751 c          828 g          283 t
ORIGIN      
        1 gaagcgcagc gtgccgttct cggcgcgggt cgccaggcgg taggccgacg ggaccgcgcc
       61 gatgacgccg accggcagca ggatcgccag agaggcgccg ccggtgaacg gcgcggcggc
      121 gacggcggcg atcgagatcg ccatcgccgc gttctcgatg gtctccatca gcagcgcggc
      181 ctggctggcc tcgatgcggc gcgagaacgt gcgaccatcg atccgcacgg atacgctgcc
      241 gcgcccgtac gcgctgcccg actcgaggat ctgccggacg ccgtcgagga gggcgctcgc
      301 gcggtcggtg ccactgccgg tggcatggcc gctgttcggg gtcgtcaggt cggagacgta
      361 gaaccgggtg tgcccgccct gggacggccg cggcgccgcc tccaggagca ggcggatcac
      421 ctggccctcg tcgctgacga acgacgcgtt gaggacctcg gagtggttca gagcgccgcc
      481 ccgctcgcgc cgcgcgcgca cctccagcac ctgctgcagc gactccagct ggcgctcgat
      541 cagcgcccgc gctctgcctc gagggcggcg cggtcggaca cctggcccgg tccggcgagc
      601 aggcgctcga tctcggccag gcgcgtgatc gcctggtcgt tcatcgcctg cgcctgctcg
      661 gtctgctgct ccgccatccg ctgtccggag gtggcgaaca ccggcaggta ggccacgctc
      721 ggcgcccgga ccacctcgtc gttgtcgccg gggatcggga cggcgcggca gcgcacgaga
      781 tagacgcctt cctcgggaaa gacgatcggt tgctcgttgt ccggccgggt ggcgatctcg
      841 aacgccaggc gaaggccggt gccgatgtag cggaagatcg cggcggcggc ggcgatctcc
      901 agcgcgccca cgcccgcctc gccgaggttg ctcgtgatgt cctggaccgc gcggccgatg
      961 tcggcgcgcg cgtagcggtt ggcgcggtcg gagtgagcgc tgcccacctc gccccgggtc
     1021 ggccgctcgc cgcgggcagt ggccgcgtcg gtgacgccgc cgatctggct gtcgggcacg
     1081 cgcacccgct cccagaagta cgaggtgcgg gcgaacgcgt cgaacaccga cgggaactgc
     1141 agctgcatga cgaaccggtg atcggtccgg agcgccgcct cgaacagcgg cggctggagc
     1201 accggatacg agctcatcgt cgatgggtgc ggctgcgcgt tggcgcggcc gggtgcgtcc
     1261 tggatcacca ggtcgccggt ggcgccgggc gtggtggcgg cgttcacgac gaagcggcgg
     1321 aaccggtcga gcagctcgcg ccgcgcgtcc gttgcttgca gcgccgccgt cgactgcgcc
     1381 ggctgggtcc gcgcgaacat caggaacagc tcggcgatgc gcgggtcggg cgcccggtcg
     1441 ggcgcggcca ggtggggcgg ctgaccctgc ggcaccggcc acagcgcgcg gtaggcgtcg
     1501 tgcgcgacca gcgccgcgtc gccgcggatc tgctcgagga cgcgcgcggg cacgttgatc
     1561 gcgtcgagca gcgcgcgcag cgccgccacc gccgccgccc ggtcgggctc ggcctgcgag
     1621 gcgcggaagc gctccagcgc cgtcacgtag tcacgcagct gcgggccgaa gcgcgcgacc
     1681 tcctggacga acaggtcgta gcgcagccac ggcggcggca ccgacgttgc cagctcggcg
     1741 gcgtcgcgcc cgggaagccg tgccaggcct gcgtggtggc catgcccagg ctgatcagct
     1801 cgcgctgggc cgcggtgacg tcgacggcga ggtgctgcgc gtcgagccag ccgagtagcc
     1861 gcgtggtgat gttctcgggg acgatgaacg tgatcccggt gagcgcgaac gacatcgcca
     1921 ccggcgtgtc ggtggtgggc gcatgtcctg tggtgggcgc gtgtccagcg acggagcctg
     1981 gcgtccggcg caccacgttc gcggcggcac cgcgacgcgc ctgcaggacg tgctcggtct
     2041 cgtgcgccag cacccagcgg ccctgcgcat caccgggggc gtaggctccg gccgagaaga
     2101 acaggtcgtc accgagcgtg aacgcgtgcg cgccgaacag
//
LOCUS       DASVOR010000880         2201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_301242,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000880 DASVOR010000000
VERSION     DASVOR010000880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301242"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="VF516_17165"
     CDS             complement(<1..563)
                     /locus_tag="VF516_17165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109466.1"
                     /translation="MTFTAIAGRERQHVIVVMLGAVADGGQVPDELALRQVIGPYGGH
                     LEQLADGSTIVVLEAGQHAATDHVARAARCALALREVANRRAMAIAMGQVESIRELTG
                     GDVLDRARRLLSRVASAPGTPTPIAIDELSAGLLDARFEVIERDDGLMLCGERALLQG
                     ARTLLGRPTPCVGRDWELDALTAILDGG"
     gene            859..1725
                     /locus_tag="VF516_17170"
     CDS             859..1725
                     /locus_tag="VF516_17170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8109467.1"
                     /translation="MPDAASARSYATVFHEIAGEYDRHRPAYPDALIDQACKLAGIGP
                     GAPVLEIGCGTGQLTRSLLARGLRVTAVEPGDQLIARARDQLQDLGDVQFVNARLEDV
                     SLPPAHYGAVFCASAIHWIDPDVSWREAASALVDRGTLALVSYFGFEEPRSAPDQQAL
                     RAAMAEIAPELAAAWPTYRDLDGTLAGIAARRDNVSEIWGWLGSYDIERRVAASLFHD
                     VQVAAVPTLLEHTADELTALLGTMSFWARLTPRQRDALAAETQALHRRLGRPIRSSTV
                     ACLVTARRQPRT"
     gene            1773..>2201
                     /locus_tag="VF516_17175"
     CDS             1773..>2201
                     /locus_tag="VF516_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109468.1"
                     /translation="MFDLPARSSLPAAPEPLVEQVLGARAIVLDELREHRVADHRVAG
                     LVALDAVVDRLEHCVADAARERREPDELQVREPRLEHEVGRDRELDRVRGEQQLVGEV
                     ARSDRDPVVGVVKLVGPVELRIPAQQLVHEQKRDLGVARLV"
BASE COUNT          328 a          798 c          755 g          320 t
ORIGIN      
        1 cccccatcga ggatcgcggt cagcgcgtcg agctcccagt cgcgcccgac gcacggcgtc
       61 ggccggccca agagcgtcct cgctccctgg agcagcgcgc gctcgccgca cagcatcaac
      121 ccgtcatcgc gctcgatcac ctcgaaccgc gcgtcgagca gaccggcgct cagctcgtcg
      181 atcgcgatcg gcgtcggggt ccccggcgcg ctcgccaccc gcgacaacag ccgccgcgcg
      241 cggtcgagca catcgcctcc ggtcagctcg cggatcgact cgacctggcc catcgcgatc
      301 gccatcgcgc ggcggttggc gacctcgcgc aacgcgagcg cacagcgcgc agcccgggcg
      361 acatggtcgg tcgcagcgtg ctggcccgcc tcgagcacga cgatcgtcga gccgtcggcg
      421 agctgttcga gatgaccgcc gtagggaccg atcacctgcc gcagggccag ctcgtccggc
      481 acctgcccgc cgtccgccac ggcccccagc atcaccacga tcacgtgctg gcgttcgcgg
      541 ccggcgatgg ccgtgaacgt cacgatccgc gcccccggcg ccgcggatcc cggcgtcgtg
      601 atgcgttgca gccccgtggg gagctcgtag gagtgagcgc gtgcatgtcg ccaccgtgtt
      661 ccaccggggt acacatgata gtcaacccgc ccgccttgca cgaggcaagt ccagcgaagt
      721 tcgggcgagg agtacacctc gacggtgcga gcgccgaggg tcgcaaggaa ctcggaggac
      781 tcggggcgac tcgcgccgtc gaccgacagc gctcctcgtg gctgacagcc gcccgccgcc
      841 gtggtctacc ttgcgaccat gccggacgca gccagcgcgc gcagctacgc caccgtcttc
      901 catgagattg ccggcgagta tgaccgtcac cgtccggcgt acccggacgc actcatcgac
      961 caggcgtgca agctggccgg tatcgggccc ggcgcgccgg tgctcgagat cggctgcggc
     1021 accgggcagc tgacacgctc tctgctggcc cggggcctgc gggtgaccgc ggtcgaaccc
     1081 ggtgaccagc tgatcgcgcg ggcgcgggat cagctgcagg acctcggcga cgtgcagttc
     1141 gtcaacgcgc gcctggagga tgtgtcgctg ccgcccgcgc actacggggc ggtgttctgc
     1201 gcgtcggcga tccactggat cgatccggat gtgagctggc gcgaggcggc cagcgcgctg
     1261 gtcgatcgcg ggaccctggc gcttgtctcc tacttcggat tcgaggagcc gcgcagcgcc
     1321 ccggatcaac aggcgctgcg cgccgccatg gccgagatcg cccctgagct cgccgccgcg
     1381 tggccgacct accgcgacct cgacggcacg ctcgcgggca tcgcggcgcg gcgcgacaac
     1441 gtgtcggaga tctgggggtg gctcggaagc tatgacatcg agcgcagggt tgcagcatcg
     1501 ctgttccatg acgtacaggt tgccgcggtg ccgaccctcc tggagcacac cgcggacgag
     1561 ctcactgctc tgctgggcac catgtcgttc tgggcacgac tgacaccccg acagcgcgac
     1621 gcgctcgccg ccgaaacgca agccctccat cggcggctcg gccggccgat ccgctccagc
     1681 accgtcgcct gccttgtcac ggcacggcgc cagccccgca cctgacccga gctcctgggc
     1741 gacgtcgccg acgagcaccg agctacgccg gcgtgttcga cctgccggcg aggtcgtcgc
     1801 tgccagctgc gccagaacca cttgtcgagc aggtactggg cgcgcgcgcg atcgtgctcg
     1861 acgagcttcg cgagcaccgg gtggccgatc atcgagtggc gggcctcgtc gctctggatg
     1921 ctgtggttga ccgtctcgaa cactgcgtcg ccgacgccgc gcgcgagcga cgcgagcccg
     1981 acgaactgca ggttcgtgaa ccccgactcg aacacgaagt tggtcgcgat cgcgaactcg
     2041 atcgggtccg aggcgagcaa cagctcgtcg gtgaggtggc gcgcagcgat cgcgacccag
     2101 ttgttggtgt ggtgaagctt gtgggtccag tcgaactgcg gatcccagcg caacagctcg
     2161 tgcatgagca aaagcgggat ctgggtgtgg cgcgcctcgt c
//
LOCUS       DASVOR010000881         3720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_388533,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000881 DASVOR010000000
VERSION     DASVOR010000881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388533"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF516_17180"
     CDS             complement(<1..555)
                     /locus_tag="VF516_17180"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8109469.1"
                     /translation="MSCVPIARLAVAVLLLPSVPALGGPTEIDLSTALARAHKLSPAA
                     IAARSGVAVAQGAVTTAELVFTENPEIDAGAGPRLTTARPIEADVRIEQNLELGRRVP
                     RRRLARAGVVQAHAEADAALRELDREVASAFYEAMFADRSAELAQRSEDLARRAAEAA
                     DRRRKAGEITDLDANLARSALGRAR"
     gene            1102..1551
                     /locus_tag="VF516_17185"
     CDS             1102..1551
                     /locus_tag="VF516_17185"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8109470.1"
                     /translation="MPARPRLSGHTLRFMRRLWELVHALAVSSKRMGRTLGVTGPQRL
                     VIRVVGQAPGATASEISDILGLHPSTLTGVLARLENQGLLQRRVDAADRRRVRFRLTP
                     AGRRIDRERKGTVEAAVRRAIGRADPQMIAHTEAVIGLLVAELGRLD"
     gene            2232..3470
                     /locus_tag="VF516_17190"
     CDS             2232..3470
                     /locus_tag="VF516_17190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109471.1"
                     /translation="MTANDAQVPSSFLQGAKTSARRLPAHTAPNGGITMAAKAQSGID
                     TLINFSSSFTTPGFDNDGNPQSVWPYTMVGRAPEQNRTTRFNAPIIPVTVELLDRNGN
                     VGTTATGAPLRMVAGQDTIDLTLNSPVFQKVDTTAGHVQLTDGVMRSEFFFRAGDGDG
                     DSDDQGYHTILDPHVKTGRTIQLPFGTYRFAPKADGSCCLFVLAEINVFANLLFPPTG
                     DDTTTVMGAAEHAGDMKTTDITTLLFRDVYLFEGVPSNCCVLGFHSVDVEPGTRQNGN
                     RERDFVMNYSSYISDGLFGGGFTDVTALSHEVAELFNDPFINNATPWWLSIDPFTGAA
                     LCQNNLETGDVVEVLSSNATAQFALHNRTYHPSNEALLQWFAFESPSSAHLGAWSFPD
                     ETTIQSLSPGPLLPGCVPSN"
BASE COUNT          594 a         1308 c         1247 g          571 t
ORIGIN      
        1 gcgggcgcgc ccgagcgcgc tgcgcgccag gttcgcgtcg aggtcggtga tctcgccggc
       61 cttgcgcctt cgatcggcgg cctccgcggc tcttcgcgcg agatcctcgc tgcgctgggc
      121 cagctcggcg ctgcggtccg cgaacatcgc ctcgtagaac gcggatgcga cctcgcgatc
      181 gagctcgcgt agcgcagcgt cggcctcggc gtgcgcctgc accacgccag ctcgtgcgag
      241 gcggcgcctg ggtacacgcc gcccgagctc gaggttctgc tcgatccgca catcggcctc
      301 gatcggccgc gcggtggtca ggcgggggcc agcgccggca tcgatctccg ggttctcggt
      361 gaacacgagc tcggcggtgg tgaccgcacc ctgcgcaacg gcgacgccgc tgcgggcagc
      421 gatcgccgca ggggacagct tgtgtgctcg ggcaagcgcg gtggacagat cgatctcggt
      481 cggtccaccc agcgcaggca cagacgggag aagcagcacg gccacggcga gccgcgcgat
      541 cggaacacaa gacatggacg taccctcagc aagccgcgct cgcgcgcggt gtcaaaacga
      601 cgtcaactcg atggatggac gagtcaaacg gcgtgccgcg gcacgtgcga gatctcgcga
      661 gggtcggggc tctggtgaac cccgtcatcg ctgtcaaacg ggggcgctgc ttgcgtcgcc
      721 ggcacgagcg tgaccgcagc gacggatgga ggaacgtgcg tctgcaggct cgggcagtgg
      781 caggggcacg acggggaatc gctcccaggc gtgcagtcgt catcgcagcc gtcgttgcgg
      841 cactcggcct cgcaggtttg ccggcgcatc gcctccgcga tgcccgttgc ctgaagcagg
      901 gcgagcacca gcagcagcgc acggggagcg gacacgggtc atccttagca ccgcgttccc
      961 cacgatacga ggagcgtgag cgcgctctgc gccggtgcgt gggtcggggg gagggggatc
     1021 cgatacggcg gcgtgctctc cgcggtaaca cgcggatcta caaggtgatc cacccgtaca
     1081 tcgttggtgt acaaaccgat catgcccgcg cggcctcggc tctccggaca caccctgcgg
     1141 ttcatgcgac ggctctggga gctggtccac gcgctcgcgg tgagctccaa gcggatgggc
     1201 cgtacgctcg gcgtgaccgg cccgcagcgc ctggtgatcc gcgtggtcgg ccaggcgccc
     1261 ggcgccaccg cgagcgagat ctcggacatc ctcggcctgc acccgagcac gctgaccggc
     1321 gtgctggccc ggctcgagaa ccagggcttg ctgcagcgcc gggtcgatgc cgcggaccgc
     1381 cgccgggtcc ggttccggct caccccggcg ggccggcgga tcgatcgcga gcgcaagggg
     1441 accgtggagg cggcggtgcg ccgcgcgatc ggccgcgccg atccgcagat gatcgctcat
     1501 accgaggccg tgatcgggct gctcgtcgcg gagctcggtc gcctcgactg agtcggattc
     1561 tgccgagcgg gagtcgagca gatcgcgaat cgacaaggaa tgatgccccc acagctcact
     1621 gtcgtcttac cgatctgtcg acaggacagg catcgcgact cagatcttcg tgaccggtgg
     1681 ggcgcgcctc ccgtcgtgtg acgccagcat ggcgcgagcc gccgcgatct gcgcgccgtg
     1741 acgagcggcg caggtggctg cggggtcgtt cgtgagatgc ttgttgcgca cggctctggg
     1801 ttgcgttcat gaagccttca cgatcgaggc tgccagcaca cccaccaagg atcttgtgtc
     1861 gatgcgggac gatctgatga gcatgttcat tcttgaaact tgactcccat gttcaattcc
     1921 tcggtcgctg cggcgcatcg cgcgcgcgcg cagcgaccgc gatgacggca gagcgtcgcg
     1981 tgcgacacca ccgtgacacc tcacggatcg gacggatcga tcctgtgagc ggtccatcac
     2041 tgcctgtgat ctcgaagtcg caatcagcag acgattgcca gagacgtcgc atgtagccga
     2101 gcacacggcg atgctacgaa gggctcggag gaatcatggg aaacaagagt attgttgcac
     2161 tactaatcgc agccgccgcg ggctgctctg actcgtcgtc agcgatcacc acggcttcca
     2221 gccagcaggc cgtgacggcg aatgatgcgc aagttccgtc gtcgttcctg cagggcgcca
     2281 agacgtccgc ccggcgcctg cccgcgcaca ctgcgcccaa cggcgggatc acgatggccg
     2341 cgaaggccca gagcggcatc gacacgctga tcaacttctc gagctcgttc accacgccgg
     2401 gcttcgacaa cgacggcaac ccgcagtccg tgtggccata caccatggtc ggccgggcgc
     2461 ccgagcagaa ccgcaccacg cggttcaacg cgccgatcat cccggtcacc gtcgagctgc
     2521 tcgaccgcaa cggcaacgtc ggcacgaccg cgaccggcgc cccgctgcgc atggtcgccg
     2581 ggcaggacac gatcgacctg accctgaact ctcccgtgtt ccagaaggtg gataccaccg
     2641 ccggccacgt gcagctcacc gacggtgtga tgcgctccga gttcttcttc cgcgccggcg
     2701 acggtgacgg tgatagcgac gatcagggct accacacgat cctcgacccc cacgtcaaga
     2761 ccggccgcac gatccagctg ccgttcggca cctaccggtt cgcgcccaag gccgacggca
     2821 gctgctgcct gttcgtgctc gccgagatca acgtgttcgc gaacctgctg ttcccgccga
     2881 ccggcgacga caccaccacc gtgatgggcg ccgccgagca cgccggcgac atgaagacca
     2941 ccgacatcac cacgctgctg ttccgcgacg tctacctgtt cgagggcgtg ccgtcgaact
     3001 gctgcgtgct cggcttccac agcgtcgacg tcgagcccgg cacccggcag aacggcaacc
     3061 gcgagcgcga cttcgtgatg aactactcga gctacatctc cgacggcctg ttcggcggcg
     3121 gcttcacgga cgtcacggcg ctcagccacg aggtggcgga gctgttcaac gacccgttca
     3181 tcaacaacgc cacgccgtgg tggctgtcga tcgacccgtt caccggcgcc gcgctgtgcc
     3241 agaacaacct cgagaccggc gacgtggtcg aggtgctgtc gtcgaacgcc acggcccagt
     3301 tcgcgctgca caaccggacc taccacccgt ccaacgaggc gctgctgcag tggttcgcgt
     3361 tcgagtcgcc gtcgtcggcc cacctcggcg cgtggagctt cccggacgag acgacgatcc
     3421 agtcgctgtc gcccggcccg ctgctccccg gctgcgtccc gtcgaactag tcgagatccc
     3481 ccgggtgcga cgccggcacc tcgccggcgt gacgcacggt gacgcaggct gaccgccgcc
     3541 tctccggcgg cgagtcgtga tactgcggag ggcatgaagc gcaccgcatt gctcggtctc
     3601 cggctcttcc tggcgctctt cctggcgccg gcgctcgcgc tcgccggctg cggttcggcc
     3661 acgacggcgt cgcaacccgc caaccgccag ccgcaggccg gcccgcggcc gccgatcgcc
//
LOCUS       DASVOR010000882         2090 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_564511,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000882 DASVOR010000000
VERSION     DASVOR010000882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564511"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF516_17195"
     CDS             complement(<1..321)
                     /locus_tag="VF516_17195"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109472.1"
                     /translation="MGAGSRSRDSRVKNGPNAAVGGGPLAAAPAAHVGATSSTDPLHE
                     TNARVGEAARKLDAAILRAQAKAALTGLGWKPAIAHAAVAAAAEAQGDTPTLERMIFE
                     ALRRC"
     gene            complement(1440..1814)
                     /locus_tag="VF516_17200"
     CDS             complement(1440..1814)
                     /locus_tag="VF516_17200"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8109473.1"
                     /translation="MNSIVSNLIVPSIEACLPFYVERLGFTRTVDVPHDGKLGFVILK
                     RGAVELMLQSCASVAADIAGLDPDGFRAALYVEVDQLSPIRKALQGWPLVVPERTTFY
                     GAREIIVRDPAGNVVCFASREA"
     gene            complement(1868..>2090)
                     /locus_tag="VF516_17205"
     CDS             complement(1868..>2090)
                     /locus_tag="VF516_17205"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8109474.1"
                     /translation="ITPKQLARIARMQRAAAALRRGGDLARLAAELGYFDQSHLSREL
                     RDLVGVTPAALATERPTVLSHLFGAPPAP"
BASE COUNT          305 a          708 c          751 g          326 t
ORIGIN      
        1 gcatcgccgc aatgcttcaa agatcatgcg ctccagcgtc ggcgtgtccc cctgcgcctc
       61 tgccgcggca gcgaccgcgg cgtgcgcgat cgcaggcttc cagcccaggc cggtcagcgc
      121 cgccttggcc tgggcacgaa ggatcgctgc gtcgagcttg cgggcggctt ctccgacgcg
      181 agcgttcgtc tcgtgtaagg gatccgtgga gctcgtcgcg cccacgtggg cggcgggcgc
      241 ggcggcgagc ggcccgccgc cgacagccgc gttcggaccg ttcttgaccc tggaatcccg
      301 cgatcgcgac cccgcgccca cgtgggcgct gtccgccggc gatccggtga tctcggccgc
      361 ctcccgagcg ccgctcgttc accaggccga cgaacctgca tatcatccgc cgtgcccgta
      421 atcgtcagcg cgccgcggtg gtgtgcgagg tggcacgcgc tgcatagcag gacgaggttc
      481 gaggcgtcat gtccaccacc atcggcgcga tggatcagat gatggatctc caacccgcgg
      541 gacgagcgac agccgtccac gcgacagcgg ccgccgtcgc ggcgccacac gaaccgcgcg
      601 acgctcggcg ggatgtcctg gtacgcccgc tccggctcgt cgccgtcgat cgagccgatg
      661 tgctgggcgt cgcacagtgc gcggtcgacc gccgcagcgt cgatcgcgat caactgcctt
      721 ccagtcgagc cgggcgtccc gcgacgggcc gggggcccac gtgggcgtcg gcgcggcgtc
      781 gatcactgca tcttcgattc cgccatccag catgaccgtc tcctgctctt gtcgcgcatc
      841 gcttctagca cacgagtttt cgacgccatg ggttgagcgc tgcgcggtga tcggaggtct
      901 ctatcaagaa attgcggccc gcccgcgatt ccgtcgttgc agcgcatgcc agcgtccttc
      961 gcgggctgat caacaggcag ttctcggcga cgtacttgat agtcgccagc agaccgtcaa
     1021 gaacaatcga ctctgcaatg cgatgatgcc agcaattgcc actctgcatc gctcactcgg
     1081 tcgaacggct tgctcgatca gcgggtcacc gggggcgcct tgttgcccga cggtggaagc
     1141 ttcgtcgcgt ccgtgtctcg ggcccctgct tgctctcgat ctccagcgcc acgcatccgc
     1201 gagatccgat gcaccgcgga tctcgccgac gctcccgtgc ggcgtgtcga agaaccgccg
     1261 cggaggacgg ccatggcgac gactgcgtcg gcgaggtcga gcacgatgct actggcgggg
     1321 cgctcgtctg accggcggcc tcggagttgc caggagcctt gctcgctcga gatctgcccg
     1381 aatgaggcaa gtttcggggc cccgcccgcc cccgcgatgc gccgtcaggc gcgttagcac
     1441 taggcctcgc gcgaggcgaa gcagaccacg ttgccggcgg ggtcgcggac gatgatctcg
     1501 cgggcgccgt agaacgtggt gcgctcgggg acgacgagcg gccagccctg gagcgccttg
     1561 cggatcgggg agagctggtc gacctcgacg tagagcgcgg cgcggaagcc gtcggggtcg
     1621 aggccggcga tgtcggcggc gaccgaggcg cagctctgca gcatcagctc gaccgcgccg
     1681 cgcttcagga tcacgaagcc gagcttgccg tcgtggggca cgtcgacggt ccgggtgaag
     1741 ccgaggcgct cgacgtagaa cggcaggcag gcttcgatgg acgggacgat caggttggag
     1801 acgatggagt tcatgccgcc aatctagggc ggccggcggg ccggggattg gagaaatgga
     1861 acctggctca cggggccggc ggcgcgccga acaggtggga cagcacggtc gggcgctcgg
     1921 tggcgagcgc ggccggcgtg acgccgacca ggtcgcgcag ctcgcgcgac aggtgggact
     1981 ggtcgaagta gccgagctcg gcggcgagcc gtgcgaggtc gccaccccgg cggagcgcgg
     2041 cggccgcgcg ctgcatccgt gcgattcgcg ccagctgctt gggggtgatc
//
LOCUS       DASVOR010000883         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_564516,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000883 DASVOR010000000
VERSION     DASVOR010000883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_564516"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VF516_17210"
     CDS             <1..714
                     /locus_tag="VF516_17210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109475.1"
                     /translation="PDPARYVEVLRAVPQLDRMALTDEDRMRAAAYAAERARTAARAA
                     AESDPDALRAHLRSLELVVRVRRIGDDDVARTAQLTQKTNQFNLTTVRRSEADVEALR
                     RDPAWRLYALDVTDRFGDYGTTGLVFAHRVDDTTWDLDTVLLSCRVLGRGVESALLRV
                     VTADLIEAGARRLTARMIPTKKNAPVRDFLPRHGFLTAPGAAPGDGPGEAFVKQPLPG
                     APFDDGHIAAQLEPGPGPR"
     gene            711..1448
                     /locus_tag="VF516_17215"
     CDS             711..1448
                     /locus_tag="VF516_17215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8109476.1"
                     /translation="MTIRDRVRHKFDRLRDQLEREDIDATSRALHIAERTLISMGLWP
                     SVSVSHERFYELTAAPRQFRGLRDLRIRLGTPDDAPAMTALDGTPGWRIAQRFVRGDL
                     VYVGEHDGRLLAHSWFHRGPEPFDEDRPLLPRWEVPADAFWSYNAFTLPEARASGVFV
                     KLFQTALRELMVDRGAARVRCRVKAANAASNSLHQRFGFAALGTLVTLSVPGARVLSW
                     EGSGRTRRWIEPRDTASVMAFPPEAGG"
     gene            1445..>1690
                     /locus_tag="VF516_17220"
     CDS             1445..>1690
                     /locus_tag="VF516_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109477.1"
                     /translation="MRLHHLGWVGNDLARMRAAFERDGAELVSPAIDDPIQRVTVQFL
                     REPATGELWELVVPLGDPAASPLASRLARGGGLDHVCY"
BASE COUNT          207 a          637 c          624 g          222 t
ORIGIN      
        1 cccgatccgg cgcgctacgt cgaggtgctg cgcgcggtgc cccagctcga ccgcatggcg
       61 ctgaccgacg aggaccgcat gcgcgccgcg gcctacgccg ccgagagggc gcggaccgcc
      121 gcgcgcgccg ccgccgagag cgaccccgac gcgctgcgcg cccacctgcg atcgctggag
      181 ctggtcgtcc gggtccgccg gatcggcgac gacgacgtcg cgcgcaccgc ccagctgacc
      241 cagaagacca accagttcaa cctgaccacc gtgcgacgca gcgaggccga cgtcgaggcg
      301 ctgcgccgcg atccggcgtg gcggctgtac gcgctcgacg tcaccgatcg gttcggcgac
      361 tacggcacga ccggcctggt gttcgcgcac cgggtcgacg acacgacctg ggacctcgac
      421 acggtgctgc tgtcgtgccg cgtgctcggc cgtggggtcg agagcgcgct gctccgggtg
      481 gtcaccgcgg atctgatcga ggccggcgcg cgccggctga ccgcccggat gatcccgacc
      541 aagaagaacg ccccggtccg cgacttcctg ccgcgccatg ggtttcttac ggcccccggt
      601 gcggcccccg gcgacggccc cggcgaggcg ttcgtcaagc agccgctgcc gggggcgccg
      661 ttcgacgacg gccacatcgc ggcccagctc gagccaggcc cggggccgcg gtgaccatcc
      721 gcgatcgcgt ccgccacaag ttcgaccggc tgcgcgatca gctggagcgc gaggatatcg
      781 acgccaccag ccgcgcgctg cacatcgccg agcggacgct gatctcgatg gggctgtggc
      841 cgtcggtgtc ggtcagccac gagcggttct acgagctgac cgcggcgccc cggcagttcc
      901 gcgggctgcg cgatctgcgg atccggctcg gcacgcccga cgatgccccg gcgatgaccg
      961 cgctcgacgg cacgcccggc tggcggatcg cccagcggtt cgtgcgcggc gacctggtct
     1021 acgtcggcga gcacgacggc cgcctgctgg cgcactcgtg gttccatcgc ggccccgagc
     1081 cgttcgacga ggaccgcccg ctgctgccgc gctgggaggt gccggccgac gcgttctgga
     1141 gctacaacgc gttcaccttg cccgaagcgc gcgcgtccgg ggtgttcgtc aagctgttcc
     1201 agaccgcgct gcgcgagctg atggtcgacc gcggcgcggc ccgggtgcgc tgccgggtca
     1261 aggccgccaa cgccgcctcg aactcgctcc accagcggtt cggcttcgcg gcgctcggca
     1321 cgctggtcac gctgtcggtg ccgggggcgc gggtgctgtc gtgggaaggc agcggccgga
     1381 cgcggcgctg gatcgagccg cgcgacaccg cctcggtgat ggcgttcccg cccgaggccg
     1441 gcggatgagg ctccaccacc tgggctgggt cggcaacgac ctggcgcgga tgcgggccgc
     1501 gttcgagcgc gacggggccg agctggtgtc cccggcgatc gacgacccga tccagcgcgt
     1561 caccgtccag ttcctgcgcg agccggcgac cggcgagctg tgggagctgg tcgtcccgct
     1621 cggcgatccg gcggcgtcgc cgctcgccag ccggctcgcc cgcggcggcg gcctcgacca
     1681 cgtgtgctac
//
LOCUS       DASVOR010000884         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417431,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000884 DASVOR010000000
VERSION     DASVOR010000884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417431"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1005)
                     /locus_tag="VF516_17225"
     CDS             complement(<1..1005)
                     /locus_tag="VF516_17225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109478.1"
                     /translation="MFYLLGEQGHHLPSFPLARALQARGHEIVYLSIRDLQHPIESRG
                     FGYVPVHEDIWPPGTLTAQAQLSREEREYAGMVRRRRMQDEPMRQRVERQIRDIDPDI
                     ALVDNIARDMVLLVHSLGVPCLRISTSLPVLADELPPITSSLAPDADHFLLELERHQL
                     PTSMSSPAVLAALSFQVARFGYPPHEVAVRTSLVPELSRYPMVVLCEEPFDLPRRGAR
                     PLYCAHRPELDRAESVDPALRDFVGEHSIILVSLGTHSGKHRHSERVYRAVADTMRAH
                     PGWRAVVAASEQFSTQGCLADPPANLLVRRHVPQLWLLRRSKVFVTHTGLGSVREAIA
                     L"
     gene            1184..1870
                     /locus_tag="VF516_17230"
     CDS             1184..1870
                     /locus_tag="VF516_17230"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8109479.1"
                     /translation="MSDDRATITFCFDDGWRNAHTRAAPILERHGHRGTFYVITQMPD
                     YMLAEGEGRMTAEQWQDLASRGHEIGCHSRTHRRFPSCSAELVADEIEGSKDDLARLG
                     LAAETFAYPYGALPRSGDGPVQQAGFRAARLYNDRSNPRTADRWRLGAHGILASHTLA
                     DAARLIQDGIDHRSWLIFAFHQIESPAPQYGCTPELLDAICAHVSERGIPVVTVRDGL
                     RWLDSTATSA"
BASE COUNT          332 a          693 c          692 g          290 t
ORIGIN      
        1 cagcgcgatc gcctcgcgca ccgatccgag cccggtgtgg gtcacgaaca ccttcgatcg
       61 gcgcagcagc cagagctggg gcacgtggcg ccggacgagc aggttggcgg gcggatccgc
      121 caggcagccc tgggtcgaga actgctcgct cgcggccacc acggcgcgcc acccgggatg
      181 tgcgcgcatc gtgtcggcca cggcgcggta cacgcgctcc gagtgccgat gcttccccga
      241 gtgggttccg agggagacca ggatgatcga atgctcgccg acgaagtcgc gcagcgccgg
      301 gtccacggac tcggcacgat ccagctcggg acgatgcgcg cagtacagcg gccgggcgcc
      361 gcggcgtgga agatcgaacg gctcctcgca caggacgacc atggggtagc gcgacagctc
      421 cggtaccagc gacgtccgga cggcgacctc gtggggcgga tagccgaagc gcgccacctg
      481 gaaggagagc gcggccagca ccgcgggact cgacatggac gtcggcagct ggtggcgctc
      541 gagctcgagc agaaaatggt cggcgtccgg ggccaggctg gaggtgatcg gcgggagctc
      601 gtcggccagc accggcagcg acgtggagat ccggaggcac ggcacgccca ggctgtgcac
      661 gaggagcacc atgtcgcgcg cgatgttgtc gaccagcgcg atgtccggat cgatgtcccg
      721 gatctgccgt tcgacccgct gtcgcatcgg ctcgtcctgc attcgcctgc gccgcaccat
      781 gcccgcgtac tcccgctcct cgcgcgacag ctgagcctgc gcggtcagcg tcccgggcgg
      841 ccagatatcc tcgtgcaccg gcacgtagcc aaaccctcgc gactcgatcg ggtgctgcag
      901 gtcgcgaatc gagagataga cgatctcgtg gcctcgggcc tggagcgcgc gggccagcgg
      961 aaacgatggg agatggtgtc cctgctcccc caggaggtaa aacatcaggc gcatcgtcga
     1021 actcccgaac tttcacgaag ttatcgcatc cctcgctgct ttcggacccg ccttgcctgg
     1081 tcgcacctgt tttccttggc tcggcccgat cgcgggaaaa acaccgcggc ggtgctggta
     1141 cgctcccgcg ggcgtcccga ccgcggacga ggagcctccg gcgatgagtg acgatcgagc
     1201 gacgatcacg ttctgcttcg acgatggctg gcgcaacgcg cacacgcgcg cggcgccgat
     1261 cctcgagcgc cacggccatc gcggaacctt ctacgtgatc acgcagatgc ccgactacat
     1321 gctcgcagag ggagaggggc ggatgacggc cgagcagtgg caggatctcg catcgcgcgg
     1381 acacgagatc gggtgtcact cacgaacgca ccgccggttt ccgagctgct ccgcggagct
     1441 cgtcgccgac gagatcgagg gctcgaagga cgatctcgca cgactcgggc tcgcggccga
     1501 gacgttcgcg tatccctacg gagcgctgcc gcgatcgggc gacggtcccg tgcagcaggc
     1561 gggcttccgc gcggctcgcc tctacaacga tcgatccaac ccgcggaccg cggaccgctg
     1621 gcggctcggc gcgcacggga tcctcgcgtc gcacacgctg gcggacgctg cgcgactcat
     1681 ccaggatggg atcgatcacc gtagctggct gatcttcgcg tttcaccaga tcgaatcgcc
     1741 ggcaccgcaa tacggctgca cgcccgagct tctcgacgcg atctgcgccc acgtgtccga
     1801 gcgcgggatt cccgtggtca cggtacgcga cgggttgcgc tggctggact cgacggccac
     1861 gtcggcctga cccgtcgcgg tacgtccggc agctggagcc cgagccgcac ccgacggaat
     1921 cgatcacacg cgcgacgttc gcgaccgcgc tcgggaccca tcggcgctac gcggaccacc
     1981 tcgcgttcct gcgaggcgaa agcatcg
//
LOCUS       DASVOR010000885         3157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_271622,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000885 DASVOR010000000
VERSION     DASVOR010000885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_271622"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1532)
                     /locus_tag="VF516_17235"
     CDS             complement(<1..1532)
                     /locus_tag="VF516_17235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109480.1"
                     /translation="MPPPPPDRTETLAPRAGCASTMPAETGPACGGDRSDPGDLAADD
                     PDRYQQVSEHARGGLGRVVRAVDRRLGRTVAVKELLRHDASNEARFLREAMITARLEH
                     PGIVPVHEAGRWPNGDPYYVMKLVEGRTLKELIAEATTLRARLGLLAHVIAVADAVGY
                     AHSQGVIHRDLKPSNVIVGAFGETIVVDWGLARDRKRDLPEPGEDILTAAGSGVSTVS
                     GKVVGTPAYMSPEQARGGEVDERADVYALGAVLYEVLAGSPPHADSTPQAVLDRVIAG
                     PPRPLSMVVPSVPDELATIVRKAMARDPDARYPNAIALAEDLRRYTTGQLVSAHSYST
                     WALLRKKLARHRNAAVVAALSGILLGAIGVTSVRRVIAERNIARRERELAEVALTSAE
                     KRKRELVLLQAETALTKDPTAALAWLKSYDVGPDERSRVVDMVDEALALGVARHVFRP
                     GDFVIDAQFTLDGSTLVAAARDGTLHGYDLATGVETLLGQVPAQPKAMVLTADGELAI
                     TG"
     gene            complement(1756..>3157)
                     /locus_tag="VF516_17240"
     CDS             complement(1756..>3157)
                     /locus_tag="VF516_17240"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8109481.1"
                     /translation="RPYQRDGVAWLQHLREHGAGGVLADDMGLGKTLQTIAHILIEKQ
                     DGRLDRPAMVVTLTSLVGNWQRELARFAPSLKVCVYHGPDRAQLRGRLAEHDVVITTY
                     PLVARDRDALAAVELHLLVLDEAHAIKNDGAQTAEAVRRLVARHRLCLSGTPIENHLG
                     ELWSLFDFLMPGLLGSRDDFQVGFREPIEARGDKLRVEALRDRVRPYILRRTKDSVAR
                     ELPGKTQLVRAVSMTGAQRELYESIRLAAHADVRRQIRTRGLAGSTIAILDALLKLRQ
                     VCCDPRLVTCDAAREVTGSAKLDVLLEMIGQGLADGRRILVFSQFARMLGLISEGLLA
                     RGVGHVTLTGSTPDRQKPIDAFQTGRADVFLISLKAGGAGLNLVGADTVIHYDPWWNP
                     AAQAQATDRAHRIGQTKPVFVHDLIIAGSVEERMLGLQRHKRDLADAILEPAARRGTR
                     LSERDVDDLLAPLADV"
BASE COUNT          407 a         1150 c         1158 g          442 t
ORIGIN      
        1 tcgccggtga tcgcgagctc gccgtcggcg gtcagcacca tcgccttggg ctgcgccgga
       61 acctggccca gcagggtctc gacgccggtc gccagatcgt agccgtgcag cgtgccgtcg
      121 cgcgccgcgg cgaccagggt cgatccgtcg agcgtgaact gggcgtctat cacaaagtcg
      181 ccggggcgga acacgtggcg cgcgaccccc agcgcgagcg cctcgtcgac catgtcgacc
      241 acccggctgc gctcgtcggg gccgacgtcg tagctcttga gccaggccag cgcggcggtc
      301 ggatccttgg tcagcgcggt ctcggcctgg agcagcacca gctcgcgctt gcgcttctcg
      361 gcggaggtca gcgcgacctc ggcgagctcg cgctcgcggc gcgcgatgtt gcgctcggcg
      421 atcacccggc ggaccgacgt gacgccgatc gcgccgagca ggatcccgct cagcgccgcg
      481 accaccgccg cgttgcggtg gcgcgccagc ttcttgcgca gcagcgccca ggtcgagtac
      541 gagtgcgcgc tgaccagctg gccggtggtg taccgccgca ggtcctcggc gagcgcgatc
      601 gcgttcgggt agcgcgcgtc gggatcgcgc gccatcgcct tgcgcacgat cgtcgcgagc
      661 tcgtccggca cgctcggcac caccatcgac agcggccgcg gcggacccgc gatcacgcgg
      721 tcgagcaccg cctgcggcgt ggaatcggcg tgcggcggtg agccggcgag cacctcgtac
      781 agcaccgccc ccagcgcgta gacgtcggcg cgctcgtcga cctccccgcc gcgcgcctgc
      841 tcgggcgaca tgtaggccgg cgtcccgacg accttgccgg acaccgtcga gactcccgag
      901 ccggccgccg tgaggatgtc ctcgccgggc tcgggcaggt cgcgcttgcg gtcgcgcgcg
      961 aggccccagt cgacgacgat ggtctcgccg aacgcgccga cgatcacgtt ggacggcttg
     1021 aggtcgcggt ggatcacgcc ctggctgtgg gcgtagccga ccgcgtcggc gaccgcgatc
     1081 acgtgggcga gcaggcccag ccgcgcgcgc agcgtggtcg cctcggcgat cagctccttc
     1141 agcgtgcggc cctcgaccag cttcatcacg tagtacgggt cgccgttcgg ccaccggccg
     1201 gcctcgtgga ccgggacgat gccggggtgc tccagccggg cggtgatcat cgcctcgcgc
     1261 aggaaccgcg cctcgttgga cgcgtcgtgg cggagcagct ccttgaccgc gacggtgcgg
     1321 ccgagccggc gatcgaccgc gcgcaccacg cggcccagcc cgccccgggc gtgctcgctg
     1381 acctgctggt agcggtccgg atcgtcggcg gcgaggtcgc cgggatccga gcgatcgccg
     1441 ccgcacgcgg gcccggtctc ggccggcatg gtcgaggcgc agccggcccg gggagccagc
     1501 gtctcggtgc gatcgggcgg cggcggcggc atggagcggc tcgagaggca ctaacctccc
     1561 acccgatcat cagattccca cgatccgggg tcggttgacg caagatcttc gatcttgtgc
     1621 ggcgggatcg gttgcctggg agggacgtct ccgcaggggt ccgagttgca cccgcagccg
     1681 gcggcggcat gtgtacgccc cggaccacat ccgatctgtg ccggagacct gtgccgggcc
     1741 ggctccgcga gtcgctcaga cgtcggcgag cggggccagc aagtcgtcga catcgcgctc
     1801 gctgagccgg gtgccgcgcc gcgccgcagg ctcgaggatc gcgtccgcca ggtcgcgctt
     1861 gtggcgctgc agccccagca tgcgctcctc gaccgagccc gcgatgatca ggtcgtggac
     1921 gaacaccggc ttggtctgac cgatgcggtg agcgcgatcg gtcgcctggg cctgggccgc
     1981 ggggttccac cacgggtcgt agtggatgac ggtgtcggcg ccgaccaggt tgagcccggc
     2041 gcccccggcc ttgagcgaga tcaggaacac gtcggcgcgc ccggtctgga acgcgtcgat
     2101 cggcttctgg cggtccggcg tgctcccggt cagcgtcacg tggccgaccc cgcgggcgag
     2161 cagcccctcg ctgatcagcc ccagcatgcg ggcgaactgc gagaacacca ggatccgccg
     2221 gccgtcggcg agcccctggc cgatcatctc gagcaggacg tcgagcttgg cgctgccggt
     2281 gacctcgcgc gccgcatcgc aggtgaccag ccgcggatcg cagcacacct ggcgcagctt
     2341 gagcagcgcg tcgaggatcg cgatcgtcga gccggccagc ccgcgggtcc ggatctggcg
     2401 gcggacgtcg gcgtgggccg cgagccggat cgactcgtag agctcgcgct gggcgccggt
     2461 catgctcacc gcgcgcacca gctgggtctt gccgggcagc tcccgggcga ccgagtcctt
     2521 ggtgcggcgc aggatgtacg gccggacccg gtcgcgcagc gcctcgacgc gcagcttgtc
     2581 gccgcgcgcc tcgatcggct cgcggaagcc gacctggaag tcgtcgcggc tgccgagcag
     2641 ccccggcatc aggaagtcga acagcgacca cagctcgccg aggtggttct cgatcggcgt
     2701 ccccgacagg cacagccggt ggcgcgcgac gagccggcgc accgcctcgg cggtctgcgc
     2761 cccgtcgttc ttgatcgcgt gggcctcgtc gagcaccagc aggtggagct ccacggcggc
     2821 cagcgcgtcg cggtcgcgcg cgaccagcgg ataggtcgtg atcacgacgt cgtgctcggc
     2881 gagccggccg cgcagctggg cgcgatccgg cccgtgatag acgcagacct tgagcgacgg
     2941 cgcgaacctc gccagctcgc gctgccagtt gccgaccagg ctggtcagcg tcaccaccat
     3001 cgccggccga tcgagccggc cgtcctgctt ctcgatcagg atgtgcgcga tggtctgcag
     3061 cgtcttgccc agccccatgt cgtcggcgag caccccgccg gcgccgtgct cccgcaggtg
     3121 ctggagccag gccacgccgt cgcgctggta cggccgg
//
LOCUS       DASVOR010000886         3108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447048,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000886 DASVOR010000000
VERSION     DASVOR010000886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447048"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VF516_17245"
     CDS             <1..775
                     /locus_tag="VF516_17245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109482.1"
                     /translation="LIAKPIAGAGSADTYRVDDAAALDAALARMHHVTEASCEEFVAG
                     DEYTFDTVCIAGAPAFENVAQYLPRPLDARSNEWISPVIITVRDLAQPRLAAGLALGR
                     QVLGALGMGDGFTHMEWYRTASGEVVFGEIGCRPGGAHLVDQMNYTCDIDLFREWARA
                     VCWGRFEAPTARKYNAAIIFKRAKGHGRITRIDGLAEFLRRHGEHVVEQRLLPPGSPR
                     RDWKKTLVSDGFLLLRHPDWDRTYQLAMEAATDITMWAE"
     gene            838..1401
                     /locus_tag="VF516_17250"
     CDS             838..1401
                     /locus_tag="VF516_17250"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HEX8109483.1"
                     /translation="MEVYTIGHSTRTLDELLALLRAHGVRTLADIRSIRRSRTNPQFD
                     EPALRKALGRRYVVIDELGGRRGKPKRPPRLANDAWDHPSFRNYADHALTAEFRAGLT
                     RLLRLPGPVAIMCAEAVWWRCHRRIVADHLLARGVAVAHILSERPATPATLTPFAVIT
                     RGRVHYPRCGGLLDPPQDSRLASSPRS"
     gene            2411..>3108
                     /locus_tag="VF516_17255"
     CDS             2411..>3108
                     /locus_tag="VF516_17255"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8109484.1"
                     /translation="MSAALESAGHDHGVHKVKHVIVIMQENHSFDNYFGALAYAPGSP
                     YHSPRRERGDGDGDADDRGGCQADDHDCVDGLSCQVDAAGTFHCSNTNPDDDGSTVHA
                     FHNPVRCVVPDLDHGWAGTHLQVNFDHPTDTLHQQLMNGFVRENDVREQPDGGVETPT
                     DDGTMGFYNQDEIPFYYELAQQFAIDDRYFSSVLGPTFPNRSYLMTATSFGHLTTQDE
                     FPPPGGYKPITGTI"
BASE COUNT          513 a         1094 c         1023 g          478 t
ORIGIN      
        1 gctgatcgcc aagccgatcg ccggcgcggg cagcgccgac acctaccggg tcgacgacgc
       61 ggccgcgctc gacgccgcgc tggcgcgcat gcaccacgtc accgaggcca gctgcgagga
      121 gttcgtcgcg ggcgacgagt acacgttcga caccgtgtgc atcgccggcg cgccggcgtt
      181 cgagaacgtc gcgcagtacc tgccgcgccc gctcgacgcg cggtccaacg agtggatcag
      241 cccggtgatc atcacggtgc gcgacctggc gcagcccagg ctcgccgccg gcctcgcgct
      301 cgggcgccag gtcctcggcg cgctcggcat gggcgacggc ttcacccaca tggagtggta
      361 ccgcacggcg agcggcgagg tcgtgttcgg cgagatcggc tgccggccgg gcggcgcgca
      421 cctggtcgat cagatgaact acacctgcga catcgacctg ttccgcgagt gggcgcgcgc
      481 ggtgtgctgg ggccggttcg aggcgccgac cgcgcgcaag tacaacgccg cgatcatctt
      541 caagcgcgcc aagggccatg gccggatcac ccggatcgac ggcctggccg agttcctgcg
      601 ccgccacggc gagcatgtcg tcgagcagcg cctgctgccg ccgggcagcc cgcgccgcga
      661 ctggaagaag acgctggtca gcgacggctt cctcctcctc cgccaccccg actgggaccg
      721 cacgtaccag ctcgcgatgg aggccgccac cgacatcacg atgtgggccg agtgaacgac
      781 cgcccgtgag cgcatgagcg cactcgttgg cgccgcgtgg cggcggtagc cttgggagtg
      841 gaggtctaca cgatcggcca ctcgacgcgc acgctcgacg agctgctcgc cctgctccgc
      901 gcccatggcg tgcggacgct ggccgacatc cgatcgatcc ggaggtcacg gaccaacccg
      961 cagttcgacg agcccgcgct gcgcaaggcg ctcggccggc gctacgtggt gatcgacgag
     1021 ctcggcggcc ggcgcggcaa gccgaagcgc ccgccgcggc tggccaacga cgcctgggac
     1081 cacccgagct tccgcaacta tgccgaccac gcgctcaccg ccgagttccg cgccgggctg
     1141 acccggctgc tccgcctgcc cggaccggtc gcgatcatgt gcgccgaggc ggtgtggtgg
     1201 cgctgccacc ggcgaatcgt cgccgatcac ctgctggccc gtggcgtcgc cgtcgcccac
     1261 atcctgtccg agcgccccgc gaccccggcg acgctgaccc cgttcgcggt catcacgcgc
     1321 ggccgcgtgc actatccgcg gtgcggcggg ctgctggatc cgccgcagga ttcgcggctg
     1381 gccagttcac cacgatcatg atacccgcgg cagcaagaac gatctcccga tcgaaattcg
     1441 tgacctccac gccactgccc gtccacacct ggaagtgtgt ggcctgaaat tccagacatc
     1501 ggttggcgca gatccgactg cgatgggcga tcacgatgtc ctggggcggt ggtccattaa
     1561 catccagctc ggtgcgcggg agtatgaacg tgagatcatc ctgttggtca cactgctggc
     1621 aaacattgac ttcctggttg cctcctcata acgatgcgcc tcgaaggagg ccttatgaaa
     1681 cacatcctga agtcagcgtt actgtcgggc gccctcacgc tatgtgcgac gatgttcggt
     1741 tgcagtaact tctcgccgcc cagtgacccc gggacagagg gggagatctc gtctgagatc
     1801 gagagtgcgt gcgactcatt ctgccagaac caaggcggct cgttctaccg cgcgacgtcc
     1861 gccacgtctc agagcgtatg tacgctacag agcggtcagt ggtttacagc tgacgatggc
     1921 tacaccgggt cgcagccggg atgttgctgc aagtgcacgc tgtcgtccgg gaactgtttg
     1981 taggcaaggc gttgccagtc gagcgtgacc cccgcaaacg gatcatcacg ccgccaggcc
     2041 tccggaccgt ctgagcgcct gttcgacgtc gtcgagcgcg ggttcggcga tgggaccgcg
     2101 acgaacagcc tggcgtgcag ccggcctctg ccgcctgtcg ggtcgtgaag taaccctcct
     2161 gggcgctcgc ggtctcgaac aggcggtccc agctcggctt tctggcgggg cggggtcgcg
     2221 aaagtgccgt ctcacggcag ctgaatgtcg cgagatcttc acccagcaca gcattctacc
     2281 gccgcaccga aatgtcatcc tcgatcacac accaccccgg gagcggacca tggtctcacg
     2341 agctgtttgc tgtgccctgc cctttgccgc ggtgctcgcc gcgtgcggcg tcgacgagcc
     2401 gatcgcggac gtgagcgccg cgctcgagag cgccggccac gatcacggcg tgcacaaggt
     2461 gaagcacgtc atcgtgatca tgcaggaaaa ccactcgttc gataactact tcggcgccct
     2521 ggcgtacgcg ccgggcagcc cgtatcacag cccgcgccgc gagcgcgggg acggcgacgg
     2581 cgacgccgac gaccgcggcg gctgccaggc ggacgaccac gactgcgtcg acggcctgag
     2641 ctgccaggtc gacgccgcgg gcacgttcca ctgcagcaac accaaccccg acgatgatgg
     2701 cagcacggtg cacgcgttcc acaacccggt gcgctgcgtg gtgccggatc tcgatcacgg
     2761 gtgggccgga acgcatctcc aggtcaactt cgaccacccg acggacacgc tgcaccagca
     2821 gctgatgaac ggcttcgtgc gcgagaacga cgtccgcgag cagcccgacg gcggcgtcga
     2881 gaccccgacc gacgacggga cgatggggtt ctacaaccag gacgagatcc cgttctacta
     2941 cgagctcgcg cagcagttcg cgatcgacga tcggtacttc tcgtcggtgc tcggcccgac
     3001 gttccccaac cgctcgtacc tgatgacggc gacctcgttc ggccacctga cgacgcagga
     3061 cgagttcccg ccgcccgggg gctacaagcc gatcaccggg acgatctt
//
LOCUS       DASVOR010000887         5086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_301341,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000887 DASVOR010000000
VERSION     DASVOR010000887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5086
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301341"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="VF516_17260"
     CDS             complement(<1..270)
                     /locus_tag="VF516_17260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109485.1"
                     /translation="MIESFVDLTYRGLSLGRRVRLSQVRPSTGYLELPAPMPVGAQIA
                     IAAEDGTAFDAVVTAVHEQVAGSDRTPGMTVAPALAAEPASAWWAA"
     gene            312..1157
                     /locus_tag="VF516_17265"
     CDS             312..1157
                     /locus_tag="VF516_17265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002637287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8109486.1"
                     /translation="MGLLDRLQQLRGAVPDDDIAGRTQALVVRGNEFGFDEFGASRES
                     LRGVLRIARWLYRHYFRASAFGVEHIPATGRVIFVANHSGQLPFDGLVIGAACFLEPP
                     QPRLVRSMVEYFVPTVPFASYLFARWGQITGTPENCRRLLAAEEAVLVFPEGARGISK
                     NFSRRYQLAEFGKGFLRLALEMRAPIVPIAVIGAEEQAPAINIKPLARLLGAPSFPVV
                     PYPPFLPLVPLPVKYRLYFGEPIRMTGDPDDDDEVLDEKARIVRNRIQSMIQIGLRER
                     EHVFW"
     gene            1179..2138
                     /locus_tag="VF516_17270"
     CDS             1179..2138
                     /locus_tag="VF516_17270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8109487.1"
                     /translation="MTEAAERPAARPRTVVVTGIAGRLGRIVARRLHHELDWRIVGLD
                     RRPMPGRPKDIEHHQVDLRSKKARDVFRAGEVDALIHLGVMHDPRARPAEIYSWNITG
                     TTKLLEYCQAYRVPKVVLLSSANVYGPRPDNPQFLTEDAPLLAAQRFPQMRDLVEIDH
                     LVSTFLWRAQGVETVILRPVHIVGPVHNAPSNYLRIQRPPVLLGFDPMVQLIHYQDIA
                     DAIVAALGPGRRGIYNIVGPGEVPLSAVLRELGREPVQIPHPVARPILSVAFRLGISS
                     FPVAELDFIRYVCMVDGRRANAELGFRPRFGLRETIHAVDEPA"
     gene            2577..3362
                     /locus_tag="VF516_17275"
     CDS             2577..3362
                     /locus_tag="VF516_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109488.1"
                     /translation="MNKPLSSLGVAIAVATVSLLAGCQLYFGSSGSSGGGGGGGANGG
                     GSDGNPPGAKCTMDNQCSAGCFCASGVCTEGGFCATDKDCGGGFHCDTSRSSCIPNPQ
                     CTADAQCKQGSMCDTKSGACFATCLCTNDADAIKAGFGWCDETRSTCMTGSDPAGACL
                     GAVSCTTAVPACPENQVPLIKDGCFTGKCRAITACEGAPVCGSLQHQDDCTARNTDCT
                     PIFAGHNCHGTTCGVSDADCVCDSYTFASCDVKGTPVTIVPGN"
     gene            3483..3920
                     /locus_tag="VF516_17280"
     CDS             3483..3920
                     /locus_tag="VF516_17280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109489.1"
                     /translation="MGFQFAETMAGTVEWAAEPGIKHPFSFEITAEAASTREYLHDGK
                     ATLRGVVHAPPLARAAEAEGVITIRPIDQRIIRYELHFTGDDGKPYELLGQKDIRWRS
                     PVHSWTYLPADILDAGHRKVGSCETRFDLQRDGWSFLRSFRRL"
     gene            complement(3927..>5086)
                     /locus_tag="VF516_17285"
     CDS             complement(3927..>5086)
                     /locus_tag="VF516_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109490.1"
                     /translation="EVKYNTLSSLARLFELGDEGMKAYALQMKTRLFATAVFPQPYKD
                     QFDTNPAVKEEVSTIVGGVSDLIRASSSDTVISVAARQGAAFADALFGVIGGDITADY
                     LRGKDYPHYKMLSLGLAGAGATNYLTFVKTKRAKLLQYKAAIHLDRYDEESRKTNALN
                     QNRIEVLTAKATELEDELRTKLTEQASTAATLAKLTEFQTEIATIEAAIDLDFGVGQG
                     EIQDQLRELQGDETAHNSYAGGGVEDGASVSDVYAVGLACSLFALLAVVPGFMGANSA
                     PELHKILRRTEKLKAYDEDANVAQIRLLAGLKPTMEGGRRLEAIIQEILDAGNARPNF
                     IADPTGQSHTFAVLWVNGAYRPKDNGESDSRTDIMNTRAFREKTIGTTWRL"
BASE COUNT          699 a         1865 c         1694 g          828 t
ORIGIN      
        1 cgcggcccac cacgccgacg cgggctcggc ggcgagcgcc ggcgccacgg tcatgcccgg
       61 cgtccggtcc gagccggcga cctgctcgtg gaccgccgtc acgaccgcgt cgaacgccgt
      121 tccgtcctcg gcggcgatcg cgatctgcgc gccgaccggc atcggcgccg gcagctccag
      181 gtagccggtg ctcggccgta cctggctcag ccggacccgc cggccgagcg agagtccccg
      241 atacgtgagg tcgacgaagc tttcgatcac accgcgatca tatcgcaccg cggccgtgca
      301 tactgccggt cgtgggcctg ctcgatcgcc tgcaacagct ccgaggcgcc gtgcccgacg
      361 acgacatcgc cggccgcacc caggcgctgg tcgtgcgcgg caacgagttc gggttcgacg
      421 agttcggcgc atcgcgcgaa tcgctgcgcg gcgtgctgcg catcgcgcgc tggctgtacc
      481 gccactactt ccgcgccagc gcgttcggcg tcgagcacat cccggcgacc ggccgggtga
      541 tcttcgtcgc caaccactcc ggccagctgc cattcgacgg cctggtgatc ggcgcggcgt
      601 gcttcctcga gccaccccag ccgcgcctgg tgcgctcgat ggtcgagtac ttcgtgccga
      661 ccgtgccgtt cgcttcgtac ctgttcgcgc gctggggcca gatcaccggc acaccggaga
      721 actgccgccg cctgctcgcg gccgaggagg ccgtgctggt gttccccgag ggcgcgcgcg
      781 ggatctcgaa gaacttctcc aggcgctacc agctcgccga gttcggcaag ggcttcctgc
      841 ggctcgcgct cgagatgcgg gcgccgatcg tcccgatcgc ggtgatcggc gccgaggagc
      901 aagcgccggc gatcaacatc aagccgctgg cgcggctgct cggcgcgccg tcgttcccgg
      961 tcgtgcccta cccgccgttc ctgccgctcg tcccgctgcc ggtcaagtac cggctgtact
     1021 tcggcgagcc gatccggatg accggcgacc ccgacgacga cgacgaggtg ctcgacgaga
     1081 aggcgcgcat cgtcaggaac cgcatccagt cgatgatcca gatcggcctg cgcgagcgcg
     1141 agcatgtgtt ctggtgagcc gatgccgcgg gggcggcggt gaccgaagcg gccgagcggc
     1201 cggccgcgcg cccgcgcacg gtcgtggtca ccgggatcgc ggggcggctc gggcggatcg
     1261 tcgcgcgccg cctccaccac gagctcgact ggcggatcgt cgggctcgac cgccggccga
     1321 tgccggggcg gcccaaggac atcgagcacc accaggtcga tctgcgcagc aagaaggcgc
     1381 gcgacgtgtt ccgcgccggc gaggtcgacg cgctgatcca cctcggcgtc atgcacgacc
     1441 cgcgcgcccg gccggccgag atctactcct ggaacatcac cggcacgacc aagctgctcg
     1501 agtactgcca ggcgtatcgc gtgcccaagg tcgtgctgct gtcatcggcc aacgtctacg
     1561 gcccgcgccc ggacaaccca cagttcctca ccgaggacgc cccgctgctc gccgcccagc
     1621 gcttccccca gatgcgcgat ctggtcgaga tcgaccacct ggtgtcgacc ttcctgtggc
     1681 gcgcgcaggg cgtcgagacc gtgatcctgc gcccggttca catcgtcggc ccggtccaca
     1741 acgcgccgtc gaactacctg cggatccagc ggccgccggt gctgctcggc ttcgatccga
     1801 tggtgcagct gatccactac caggacatcg ccgacgcgat cgtggcggcg ctcggccccg
     1861 gccggcgcgg gatctacaac atcgtcgggc cgggcgaggt gccgctgtcc gccgtgctcc
     1921 gcgagctcgg ccgcgagccc gtgcagatcc cacacccggt ggcaaggccg atcctgtcgg
     1981 tcgccttccg gctcgggatc tcgagcttcc cggtcgccga gctcgacttc atccgctacg
     2041 tctgcatggt cgacggccgc cgcgccaacg ccgagctcgg ctttcgcccg cgcttcgggc
     2101 tgcgtgagac cattcacgcg gtcgacgagc cggcgtagcg ccgcgccgtc gccccggtgg
     2161 gcgctttgcc ccgcgttgcc cggcgctccc gcctgccgcg ttcggttgcc gccgtcacgg
     2221 atctgccgcc aaccgaacag acggcggcgt cgcgtgtgtc gtcctgcggt gtggtgccgg
     2281 cgtggcgtct cgatacaggg agtgtggccc gacgttcaca cccgacgagg cagcacggcc
     2341 gccgggcgcc cgcttgccac cggcgctcgt cggtagcccg cgccgcctcc gcgccgccga
     2401 tgccgcaagc agtggtggtt tttcgggcag ttttgtcaga tcctctcctc gtacgacccg
     2461 tcggccccac cgccgcgtgt cgaaatcttg ccgcattgtc cacgcgctcc gcggtcttgc
     2521 ctgcatggca cgaccgatgc ttcgcgctgg taccgcacgc caaaggacgc ccccccatga
     2581 acaagccgct ctcttcactc ggtgttgcga tcgcagtcgc cacggtctcc ctgctcgctg
     2641 gctgccagct ctacttcggc tccagcggct cttcgggagg cggcggtgga ggcggcgcga
     2701 acgggggtgg ctccgacggc aaccccccgg gcgcgaagtg cacgatggac aaccagtgct
     2761 ccgccggctg cttctgtgcc agcggcgtct gtaccgaggg cggcttctgc gccacggaca
     2821 aggactgcgg tggtggcttc cactgcgaca ccagccggtc atcctgtatc cccaacccgc
     2881 agtgcacggc ggacgcacag tgcaagcagg ggtcgatgtg cgacaccaag agcggcgcct
     2941 gctttgcgac ctgcctctgc accaacgacg ccgacgcgat caaggccggc ttcggctggt
     3001 gtgacgagac gcgcagcacg tgcatgaccg gcagcgatcc ggcgggcgcg tgcctcggcg
     3061 cggtcagctg caccacggca gtcccggcct gccccgagaa ccaggtgccg ctgatcaagg
     3121 atggctgctt caccggcaag tgccgcgcga tcaccgcgtg cgagggcgct ccggtgtgcg
     3181 gctcgctgca gcaccaggat gactgcaccg cccgcaacac cgactgcacg ccgatcttcg
     3241 ccgggcacaa ctgccacggc accacgtgcg gcgtcagcga cgcggactgc gtgtgcgact
     3301 cctacacctt cgcgtcgtgc gacgtgaagg gcacgccggt gacgatcgtc ccgggcaact
     3361 gaacgcgcat ccggccgggt cgtccggcgc gcaagggagg tcgccatccg gcggcctctt
     3421 ctgcatttcg ggcgtttcgg ccgtttcggg cgcttcggcg gcccatcggc taggcttgcg
     3481 gtgtgggttt ccagttcgcc gagacgatgg ccggcaccgt cgagtgggcg gccgagccgg
     3541 gcatcaagca tccgttctcg ttcgagatca ccgccgaggc agcgtcgacc cgcgagtacc
     3601 tgcacgacgg caaggccacg ctgcgcggcg tcgtccacgc gccgccgctg gccagggccg
     3661 ccgaggccga gggcgtcatc acgatccggc cgatcgacca gcgcatcatc cgatacgagc
     3721 tgcacttcac cggcgacgac ggcaaaccct acgagctgct cgggcagaag gacatccggt
     3781 ggcgctcgcc ggtccacagc tggacctacc tgcccgccga catcctcgac gccggtcacc
     3841 gcaaggtcgg cagctgcgag acccgcttcg atctccagcg cgacggctgg agcttcctgc
     3901 gcagcttccg ccggctgtga cgcccgctac agccgccagg tcgtgccgat cgtcttctcc
     3961 cggaacgccc tcgtgttcat gatgtcggtg cgcgagtcgg actcgccgtt gtccttgggc
     4021 cggtaggcgc cgttgaccca gagcaccgcg aaggtgtggg actggccggt cggatccgcg
     4081 atgaagttcg gccgcgcatt gcccgcatcg aggatctcct ggatgatcgc ctcgagccgg
     4141 cggccgccct ccatcgtcgg cttgaggccg gcgagcaggc ggatctgcgc cacgttggcg
     4201 tcctcgtcgt aggccttcag cttctcggtc ctgcgcagga tcttgtgcag ctccggcgcg
     4261 ctgttcgccc ccatgaagcc ggggaccacc gccagcagcg cgaacaggct gcacgccaga
     4321 cccaccgcgt agacatcgct gaccgacgcg ccgtcctcga cgccgccgcc ggcgtagctg
     4381 ttgtgcgcgg tctcgtcgcc ctggagctca cggagctgat cctggatctc cccctggccc
     4441 accccgaagt cgagatcgat cgcggcctcg atcgtggcga tctcggtctg gaactcggtc
     4501 agcttggcca gggtcgcggc ggtgctcgcc tgctcggtca gcttcgtcct cagctcgtcc
     4561 tcgagctcgg tcgccttcgc ggtcaggacc tcgatccggt tctggttcag cgcgttggtc
     4621 ttgcgggact cctcatcata ccggtcgagg tggatcgccg ccttgtactg gagcagcttg
     4681 gcccgcttcg tcttgacgaa ggtcaggtag ttggtcgcgc cggcgccagc gaggccgagc
     4741 gacagcatct tgtagtgcgg gtagtccttc ccccgcaagt agtccgccgt gatgtcgccg
     4801 ccgatgaccc cgaacagcgc atcggcgaac gcggcgccct gccgggccgc caccgagatc
     4861 acggtgtccg agctcgacgc ccggatcaga tcgctcacac cgccgacgat ggtggagacc
     4921 tcctccttga cggcggggtt ggtgtcgaac tgatccttgt atggctgcgg gaacaccgcg
     4981 gtcgcgaaca gccgcgtctt catctgcagc gcgtacgcct tcatgccctc gtcgccgagc
     5041 tcgaacagcc tcgccaggct gctgagcgtg ttgtacttga cctcct
//
LOCUS       DASVOR010000888         1711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_155075,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000888 DASVOR010000000
VERSION     DASVOR010000888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1711
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155075"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(624..1565)
                     /locus_tag="VF516_17290"
     CDS             complement(624..1565)
                     /locus_tag="VF516_17290"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8109491.1"
                     /translation="MKALLVAMVAIAIGCSSKPSPPPSSLIEDAGAGDGGLDPAELAA
                     LYDHKCVAGDLEACRNLGVMYAEGTGVSVDRQRSAKLFLQACNGGNLSACNNLALAYL
                     EGTGVERQPTRAAEVLQKACDAGYKLACRNLGLLLRDGRALPADLVRAQVLLDKACKG
                     GVPLACTNAGDVDAMRAVKGGAARYKEMVAHYKQGCDAGDPTSCRQLGIAYLEGRGLP
                     KSTSAAALWLERACMPDDPVACRLLGILRLQGAGVARDGERGRQLLVRACDAKDDEAC
                     RMLQVMESGLPVLPGDDAGVGDGGSANPADARISPGP"
BASE COUNT          238 a          630 c          571 g          272 t
ORIGIN      
        1 tgtttcgcga tcctgccgcg ctcgccgcga tccgccgcgc ggcgatggcg cgcgactcgt
       61 cctggaccgc atccgcgcag cagtacgtcc agctctaccg ctcgctcatc gcgtgacgcg
      121 gctgcgacca tcaggctgtc gcgttgtcac atggacgccg ggttgtccac ccatcgagcc
      181 tggccccggc tggacgccgg tacgtccacc ccgtcgatct cccgacaggc gttctgccag
      241 gcagatctgc cgcagaatgc ctcagatagg gagatcagcg tccgggctcc ggacggttcg
      301 gtccgcgggt cggacggctg cgtccggtgt gcggactggc ccgtccgccg atcggaccgc
      361 tacacccggg ctgtgcgccg aacaccagtc cggagtgcgg acgacgggcc gttttcccct
      421 gtcaacaact ggccccccgg gccgggttat tcacaaccag gcaagagcta actgcatgat
      481 tcgcaaggct ctgagcctcg ctctcgcgtc atgtgaataa gctgtgatcc gtgtgtgagg
      541 cccccctgcg ggagcgatga cggttcagcc acttacgcgt catgtcatgc gatctgctgt
      601 gttatcttac acgccgcgga cgatcatggt ccgggcgaaa ttctcgcgtc cgctgggttt
      661 gccgacccac cgtcacccac gccggcatcg tctccaggga gcacagggag gccgctctcc
      721 atcacctgga gcatccggca ggcctcgtcg tccttcgcat cacaagcgcg caccagcagc
      781 tgccggccgc gctcgccgtc ccgcgcaact cctgcgccct gcagccgcag gatgccgagc
      841 agacggcacg ccacggggtc atcgggcatg caggcgcgct ccagccagag cgccgcggcg
      901 ctggtcgatt tcggcagccc cctgccctcc aggtaggcga tgccgagctg gcggcacgac
      961 gtgggatcgc cggcgtcgca gccctgcttg tagtgcgcga ccatctcctt gtagcgcgcg
     1021 gcgccgccct tgaccgctcg catcgcgtcg acgtcgccgg cgttggtgca cgcgagcggg
     1081 acgccgccct tgcacgcctt gtcgagcagc acctgggcgc gcaccaggtc ggcgggcagc
     1141 gcgcggccgt cgcgcagcag caggccgagg ttgcggcacg ccagcttgta gccggcgtcg
     1201 cacgccttct gcagcacctc ggctgcccgg gtcggctggc gctcgacccc ggtgccctcg
     1261 aggtaggcga gcgcgaggtt gttgcaggcc gacaggttgc cgccattgca cgcctgcagg
     1321 aacagcttgg ccgatcgctg gcggtcgacc gacacgccgg tgccctcggc gtacatcacg
     1381 cccaggttgc ggcaggcctc gaggtcgccg gcgacgcact tgtggtcgta cagcgccgcg
     1441 agctcggccg gatcgaggcc cccgtcgccg gcgcccgcat cctcgatcaa cgacgacggc
     1501 ggcgggctcg gcttgctgct gcagccgatc gcgatcgcga ccatcgcaac gagcagagcc
     1561 ttcacggagt ccgttggtat cacaggtcga gcaccgcggg gccctgcacc gcggcgcgca
     1621 ccgtccggta cgccgcgtcc gcccgcggct cccggtgcgg gatggcgcca tagccggtga
     1681 ggatcgccgc ggtcaccacc gccgcgatcg c
//
LOCUS       DASVOR010000889         3165 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_359663,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000889 DASVOR010000000
VERSION     DASVOR010000889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359663"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF516_17295"
     CDS             <1..241
                     /locus_tag="VF516_17295"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109492.1"
                     /translation="LEFGVMRDGGALKVYGAGLLSSFGECGRFETAAELVPFDLDVMA
                     DTPFDPTDYQRTLFVAPSFEVMQRELLAWLARRAR"
     gene            complement(308..862)
                     /locus_tag="VF516_17300"
     CDS             complement(308..862)
                     /locus_tag="VF516_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109493.1"
                     /translation="MIVSLRALIASAGAGLAACGAPAASVVKDEPPAPALALDLSFTG
                     TLGTPGLVAHCAAKNISSQPVHVFDSARMPYLIVDQGTLVVLHGVSPPGDRLLGAIEI
                     PLTRVLPPGETLAFDVPLAPLHLRSHYGDEPDPGLHGATPIVCRVAHGATPIDAAARA
                     HTTITALLAWQQFDASPTLVVKLP"
     gene            2176..2919
                     /locus_tag="VF516_17305"
     CDS             2176..2919
                     /locus_tag="VF516_17305"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8109494.1"
                     /translation="MRSSRSKREPKRRGQRELFRRGGKRRGAGRKPKGARAGERHQAR
                     PEFKPYHPLHVVMRVAPEVGSLWRRAMYKALRDATITAALRERFRIVHISLQRTHVHL
                     LVEADHKTALARGMQGFTISAARHINTALGSGARRRRGKVFADRYHVEVITTPTRARH
                     ALGYVLGNWRKHGEDRDGLASTWLVDPFSSGISFPDWAELEGQPWMWPIRETYDPLMV
                     RRPQTWLLREGWKVGGRAISARDVPSKPR"
     gene            2897..>3165
                     /locus_tag="VF516_17310"
     CDS             2897..>3165
                     /locus_tag="VF516_17310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109495.1"
                     /translation="MCRASRADAPRSGENDPQQGAAVRPTCGRPARPARGGWFIDDRH
                     QREDATQARVRATRYPTDERVCWMPAGRHDRTAVLPLSGRMPAAGA"
BASE COUNT          510 a         1046 c         1127 g          482 t
ORIGIN      
        1 gctggagttc ggggtgatgc gcgacggcgg cgcgctcaag gtctacggcg cgggcctcct
       61 gtcgtcgttc ggcgagtgcg gccggttcga gaccgcggcc gagctcgtgc cgttcgacct
      121 cgacgtcatg gccgacacgc cattcgaccc gaccgactac cagcgcacgc tgttcgtcgc
      181 cccgagcttc gaggtcatgc agcgcgagct gctggcctgg ctggcccgcc gcgcgcggtg
      241 aggagccccg aggctgcgct cgaggctgcg cccgaggctg tgcccgaggc tgtgcccgag
      301 gctgtgatca aggcagcttg acgaccaggg tcggcgacgc gtcgaactgc tgccacgcga
      361 gcagcgcggt gatcgtcgtg tgcgcgcgcg ctgcagcgtc gatcggcgtc gcgccgtggg
      421 cgacccggca cacgatcggc gtggcgccgt gcaaccccgg gtcgggctcg tcgccgtagt
      481 ggctgcgcag gtggagcggc gcgagcggga cgtcgaacgc gagcgtctcg cccggcggca
      541 gcacccgggt cagcgggatc tcgatcgcgc cgagcaggcg gtcgccgggc ggcgacacgc
      601 cgtgcagcac gaccagcgtg ccctggtcga cgatcaggta cggcatccgc gccgagtcga
      661 acacgtggac cggctgactg gagatgttct tcgcggcgca atgcgcgacg agcccgggcg
      721 tgccgagcgt gccggtgaag ctgaggtcca gcgcgagcgc gggggccggc ggctcgtcct
      781 tgacgacgga ggccgccggc gcgccgcacg cggccagccc ggcgccggcg ctggcgatca
      841 gcgcgcgcag gctcacgatc atggccacgg atcggcgccg catgcgtcca tttgcgtaac
      901 acgaaccgac gacgcggctc aagccggccg ccgcccgagg gtcgtccgtg cattcacgaa
      961 cgaggctcgc gcggatcttt tcagtgccac tcgacgtgga ggatgctcgc cggcgcctcg
     1021 gcggcctggc cagcacccct gacccgaagc ccgccccgca acagcgctgt cgtcgccggc
     1081 acccgaaggc cgccgcgcat gcccttccgg gatcccgaac caaaaatcga tccggaaggg
     1141 agcgttcgcg cggggcgatg cactgtgaat gggtcgtcct ggtcggcctg gtcggcttca
     1201 accggccagt cacagggccg tccacgcggt ctcccgaccc atggcccagc cggctgcgac
     1261 gaggatggtg atccctcgtc ggctgctcga ctggacggct ccatgcgcga agctacctcg
     1321 gcggaataac ctgctgcact ccggcgtgcc tccagctgcg ccatgagcgc atggacctgc
     1381 gtgttcgcga agcggagcga tgcgcttgct tgccgagaaa tcttggtcgc aacgacggga
     1441 cagtcaaatc gccgacgagg gaccaccagg atcgtcgcag gcagctgggc catggaccgg
     1501 gagaccgtgt ggacggccct gtgactggcg cgccgcagct cacgaggtcg accaggacgc
     1561 cccactcaca gtgcatcgcc ccgcgccgac gctcccttcc aatgaatgtt gattcatttc
     1621 cggatctccg gagaagggct cgctcggcgc gagtagcggt gcgcggagct gtctcggatc
     1681 agtgacctgc tgcgctccgg cgcgcctcca gaatcatgat cgcaagatgc gtttttcttc
     1741 ctctggatcg gagagcctaa aatatataaa tgcaagaaag aggcctggag tcgaggcgag
     1801 gactctggga aatggaagga cgactgtgag ccgaggcggc gtcgacctcg tttggcgcgg
     1861 cgcgggtcac ccgcgtcccc gttctctatg ggccgccccg aggctctgca gcggatctcg
     1921 ctcgacgatt cgtcgaaggc agcacaacgt gttttgatcg atccgtcacg tcagcactgc
     1981 ctgcccgtcc gctaccagcg aagtgggagc tcccactgca ccgactcgtg atgcattgtc
     2041 gtcattcaag gacaagtaac ctgatccgga tacccgagag tacgtgtctc gatgacttgg
     2101 caatcgtctg gaatgaatac caattagacc tgctcaatca ttgacaagct gtgcattcgt
     2161 ggtaacgatg cgctcatgag gtcgtcgagg tcgaagcgcg agcccaagcg gagaggtcag
     2221 cgggagctgt tccggcgcgg cggcaagcga cgcggtgccg ggcggaagcc gaagggcgcg
     2281 cgtgccggcg agcggcacca ggcgcggccg gagttcaagc cgtatcatcc gctgcacgtc
     2341 gtgatgcgag tcgcgccgga ggttggcagc ctgtggcggc gggcgatgta caaggcgctg
     2401 cgcgatgcca cgatcacggc ggcgctgcgc gagcggttcc ggatcgtaca catctcgttg
     2461 cagcgcacgc acgttcactt gctcgtcgag gccgatcaca agacggcgct tgcccgcggg
     2521 atgcagggct tcacgatctc ggcggcgagg cacatcaaca ctgcgctggg gagcggcgcg
     2581 cgccggcgcc gtggcaaggt gtttgccgat cgctatcacg tcgaggtcat cacgacgccg
     2641 acccgggcgc gccacgctct tgggtacgtg ctggggaact ggagaaagca cggggaagac
     2701 cgcgacggcc tggcgagcac ctggctcgtc gatccgtttt ccagcgggat ctcgtttccc
     2761 gactgggcgg agctggaggg tcagccctgg atgtggccga tccgcgagac ctacgatccg
     2821 ctgatggtgc gaagacccca gacctggttg ctccgggagg gatggaaggt cggagggcgc
     2881 gcgatcagcg cgcgcgatgt gccgagcaag ccgcgctgac gctccccgtt cgggcgagaa
     2941 cgacccgcag caaggcgcag cggttcgacc aacctgcggc cggccggccc gcccggcgcg
     3001 cggcggctgg ttcatcgacg accggcacca gcgcgaggac gcgacccagg cgcgggtccg
     3061 cgcgacgcgc tatccgaccg acgagcgcgt gtgctggatg cccgccggcc ggcacgaccg
     3121 caccgcggtg ctgccgctga gcggaaggat gcctgccgcc ggtgc
//
LOCUS       DASVOR010000890         3155 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_213606,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000890 DASVOR010000000
VERSION     DASVOR010000890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3155
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213606"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..1205
                     /gene="ugpC"
                     /locus_tag="VF516_17315"
     CDS             33..1205
                     /gene="ugpC"
                     /locus_tag="VF516_17315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX8109496.1"
                     /translation="MATVVLRDVRKSFDTVEVIRGVSLTVSDGEFVVFVGPSGCGKST
                     LLRLIAGLETITAGEVVIDGERSNDLPPAERGIAMVFQSYALYPHMSVYDNMAFALKL
                     AHMAKDEIRDRVERAAGILQIRELLGRKPRALSGGQRQRVAIGRAIVRAPKVFLFDEP
                     LSNLDAALRVQMRIEIARLHKDLKTTIIYVTHDQVEAMTMASRIVVLNAGLVEQVGTP
                     LELYHRPGNRFVAGFIGSPKMNFLPGTLVAAEPTRARVALRDGTELTCAVDATRAAQG
                     AELTVGIRPEHTSPVSEGTAPGTAPSPYRSDPVGGQLRGEVALVENLGESALIYVRVP
                     GTEGLTLCRIEGASLAREGEPIELAVPPGVVHVFDAEGKAFPRTVDALPGAERRAS"
     gene            1382..2491
                     /locus_tag="VF516_17320"
     CDS             1382..2491
                     /locus_tag="VF516_17320"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8109497.1"
                     /translation="MSAVSISTALVATLATIGCKKPKEGGGAGGSGGSIALLLPESQT
                     TRYESADRPFFEAKFKQLCPNVDIVYSNANQVAADQQQQAEAAITRGVKVLVLDPVDG
                     DAAGAIVKSAKAKNIPVISYDRLIKGGSSPDYYISFDNEQVGKLQAQALLEKLASGGN
                     AKPKITMINGSPTDNNATLFKNGAHSVFDGKVDIVKEDAAANWKPEEAQQLMEQFIAA
                     VGKDGFDGAYCANDGTAGGAIAAMKAAGIDPKTRPICGQDAEVAAVQRILSGEQYLTV
                     YKAIQPEAEKTAELACALFKGEKPAADVVNNKVNNGTADIPSVLLTPIAVTAAGGGTT
                     KSVAETIVKDKFYGPDTVAKLCKGYEPACQAASIK"
     gene            2467..>3155
                     /locus_tag="VF516_17325"
     CDS             2467..>3155
                     /locus_tag="VF516_17325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8109498.1"
                     /translation="MSGGIDQIAGQIARTPLLELHGISKRFGAVQALEGVQFSVSAGE
                     VVALVGDNGAGKSTLIKIISGIYRADQGTIRWQGRPVAIATPNDSTALGIATVYQDLA
                     LCDNLDVVANLFLGRELATPGGPLGVMDEVTMERRAVEVLRRLSVKIPSVRIPIASLS
                     GGQRQSVAVARSVMGDNKVVILDEPTAALGMAQTHEVLELIKRLRAQGLGVVLISHNL
                     ANVYEVADRITV"
BASE COUNT          523 a         1084 c         1106 g          442 t
ORIGIN      
        1 tgcggcgacc ggctatggtc ggatcgccgc acatggccac cgtcgtcctg cgcgacgtcc
       61 gcaaatcgtt cgacaccgtc gaggtcatcc gcggcgtcag cctgaccgtc tccgacggcg
      121 agttcgtcgt gttcgtcggc ccgtcgggct gcggcaagtc gaccctgctg cgcctgatcg
      181 ccgggctgga gaccatcacc gccggcgagg tcgtgatcga cggcgagcgc tccaacgacc
      241 tgccgccggc cgagcgcggc atcgcgatgg tgttccagag ctacgcgctg tacccgcaca
      301 tgtcggtcta cgacaacatg gcgttcgcgc tcaagctcgc ccacatggcc aaggacgaga
      361 tccgcgaccg ggtcgagcgc gcggccggca tcctccagat ccgcgagctg ctcggtcgca
      421 agcccagggc gctgtcgggc gggcagcgcc agcgcgtggc gatcggtcgg gcgatcgtgc
      481 gcgcgcccaa ggtgttcctg ttcgacgagc cgctgtccaa cctcgacgcc gcgctccggg
      541 tccagatgcg gatcgagatc gcccggctgc acaaggatct caagacgacg atcatctacg
      601 tcacgcacga tcaggtcgag gcgatgacga tggcgagccg gatcgtcgtg ctcaacgccg
      661 ggctggtcga gcaggtcggc acgccgctcg agctgtatca ccggccgggc aaccggttcg
      721 tcgccggctt catcgggtcg cccaagatga acttcctgcc gggtacgctg gtcgccgccg
      781 agccgacccg ggcccgggtc gcgctgcgcg atggcaccga gctgacctgc gcggtcgacg
      841 cgacgcgcgc cgcccagggc gccgagctga ccgtcgggat ccggcccgag cacaccagcc
      901 cggtcagcga ggggacggcg ccgggtacgg cgccgtcgcc gtatcggtcg gacccagtcg
      961 gcggccagct gcgcggtgag gtcgccctgg tcgagaacct cggcgagagc gcgctgatct
     1021 acgtccgggt gcccgggacc gagggcctga cgctgtgccg gatcgagggc gccagcctgg
     1081 ccagggaggg cgagcccatc gagctcgccg tcccgccggg cgtcgtccac gtgttcgacg
     1141 ccgagggcaa ggcgttcccg cgcacggtcg atgcgctgcc cggcgccgag cgtcgcgcga
     1201 gctgagcgcg ccgtttcgcg caaagtcagc gttgcgtttg gcaacacggt ttccgtgcag
     1261 cgatcccgtg atcgcggcgt gcggctgttg atcccgggtc gcccccgcca tagggtcgag
     1321 gcgtcgcggt ccccgcccac ggtggcgctg gaggcatccc atgagactgt ccaattcgat
     1381 cgtgtcggcc gtttcgatct cgactgcgct cgtcgcgacg ctcgcgacga tcgggtgcaa
     1441 gaagcccaag gagggcggcg gcgcgggagg cagcggcggc agcatcgcgc tgctcttgcc
     1501 ggagtcgcag accacgcgct acgagtcggc cgatcggccg ttcttcgagg cgaagttcaa
     1561 gcagctgtgt cccaacgtcg atatcgtgta ctccaatgcc aaccaggtcg cggccgacca
     1621 gcagcagcag gccgaggccg cgatcacgcg cggcgtgaag gtgctggtgc tcgatccggt
     1681 cgacggcgac gccgccggtg cgatcgtcaa gagcgccaag gccaagaaca tcccggtgat
     1741 cagctatgac cgcctgatca agggcggctc gagcccggat tattacatct cgttcgacaa
     1801 cgagcaggtc ggcaagctgc aggcccaggc gctgctggag aagctggcca gcggcggcaa
     1861 cgcgaagccg aagatcacga tgatcaacgg ctcgccgacc gacaacaacg cgacgctgtt
     1921 caagaacggc gcccacagcg tgttcgacgg caaggtcgac atcgtcaagg aggacgcggc
     1981 cgccaactgg aagcccgagg aggcgcagca gctgatggag cagttcatcg ccgcggtcgg
     2041 caaggacggg ttcgacggcg cctactgcgc caacgacggc accgccggcg gcgcgatcgc
     2101 cgcgatgaag gccgccggga tcgaccccaa gacccggccg atctgcggcc aggacgccga
     2161 ggtcgccgcg gtccagcgga tcctgagcgg cgagcagtac ctcaccgtgt acaaggcgat
     2221 ccagcccgag gccgagaaga ccgccgagct ggcgtgcgcg ctgttcaagg gcgagaagcc
     2281 ggcggccgat gtcgtcaaca acaaggtcaa caacggcacc gccgacatcc cgtcggtgct
     2341 gctgactccg atcgcggtca ccgcggccgg cggcggcacc accaagtcgg tcgccgagac
     2401 catcgtcaag gacaagttct acggcccgga cacggtggcc aagctctgca aaggctacga
     2461 gccggcgtgt caggcggcat cgatcaaata gccgggcaaa tagctcgcac gccgctgctc
     2521 gagctgcacg ggatcagcaa gcggttcggc gcggtccagg cgctggaggg cgtccagttc
     2581 agcgtgtccg ccggcgaggt cgtcgcgctc gtcggcgaca acggcgccgg caagtcgacg
     2641 ctgatcaaga tcatctccgg catctaccgc gccgaccagg gcacgatccg ctggcagggg
     2701 agaccggtcg cgatcgccac gcccaacgac tcgacggcgc tcggcatcgc gaccgtctac
     2761 caggacctcg cgctgtgcga caacctcgac gtggtcgcca acctgttcct cggccgcgag
     2821 ctcgcgacgc cgggcggacc gctcggcgtg atggacgagg tcacgatgga gcgccgcgcc
     2881 gtcgaggtgc tgcgccggct gtcggtcaag atccccagcg tgcggatccc gatcgcgtcg
     2941 ctgtccggcg gccagcgcca gtcggtggcg gtggcgcgct cggtgatggg cgataacaag
     3001 gtcgtgatcc tcgacgagcc gaccgccgcg ctcggcatgg cccagaccca cgaggtgctc
     3061 gagctgatca agcggctgcg cgcccagggc ctgggcgtgg tgctgatcag ccacaacctc
     3121 gccaacgtgt acgaggtcgc cgaccggatc accgt
//
LOCUS       DASVOR010000891         6330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_301397,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000891 DASVOR010000000
VERSION     DASVOR010000891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6330
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301397"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF516_17330"
     CDS             <1..415
                     /locus_tag="VF516_17330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109499.1"
                     /translation="IDEGAEGVDLMPTVLQAIGAPASPAPQGEPLEPLAQGIGRGWAR
                     PSYASQYEYAHAMRIGRWKLRVGPRGTPLLGDMAADPGETRDAASGHPVERRMLTDNL
                     GLFLALRTQWRKAEWGVVTSVSPAGARALDGAFTP"
     gene            412..1647
                     /locus_tag="VF516_17335"
     CDS             412..1647
                     /locus_tag="VF516_17335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109500.1"
                     /translation="MTGRARPEAVLACAIGAACAACAACATQDVSPVASTSPRIADGP
                     SSSPAGDEARNEAVDELDSRESFEPGVSVESVEPIPLGPADVLSPEAEVWLKGVTHVH
                     ARASGDSSEPPGNVIGWYEQHGYDFIALTDHNRVSELDRSNDTRGQVTLRDPHAGLIV
                     FSGIELTHNPIGCLPIGDASRNCRIHVNLVGPTARPAGRIEWAERHSHLRIDMYQAAV
                     VAQTALGGIAQINHPQWLWGMTPDLLVELAHRGMPLIEIANAAFSRWNAGDVAHPSTE
                     VLWDAALARGATLWGIASDDAHDYEGHGKYPPGGGWVMVRARRDPRAILDALAAGRFY
                     ASNGVVLERAEVEGDELAIAVDPAAPGSYTIDFIENGRRVDSVHGRFARRRVPAAGYV
                     RAVVTRNDGKRAWVQPARR"
     gene            complement(1689..2309)
                     /locus_tag="VF516_17340"
     CDS             complement(1689..2309)
                     /locus_tag="VF516_17340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8109501.1"
                     /translation="MIRLAIAEDHTIVRWALREALSKADDIEVVGEAGTAAETLEMIK
                     RERPDVLILDISLPDRSGFDVLTEMRPMDTAPLVVVLTWHTELSYAARAIAAGAHGYV
                     NKAVEPDELLAAIRAVSRGEQVIPPGVEELLRTGDGHPASALTAREQQVMEMLARGMT
                     NREIAEHLEISIKTVDTHRGHVLKKLGLRNNSELTRFAVKHGYTAL"
     gene            2775..3401
                     /gene="udk"
                     /locus_tag="VF516_17345"
     CDS             2775..3401
                     /gene="udk"
                     /locus_tag="VF516_17345"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEX8109502.1"
                     /translation="MLRRPYVVGIAGGTGSGKTTVADKLAAAMPPGRCVTLDHDAYYR
                     DQGHLPPADREAINYDHPSALDSALLALHLRELRDGRTVEVPIYDFATHTRRRDTRTV
                     APARVILVEGILVFAEPALREQMDIKIFVDTDADIRLMRRIRRDLEERGRTFQSVRDQ
                     YHATVRPMHLEYVEPSKRWADLILPEGGDNRVALDVLLGQLWRVVAEP"
     gene            complement(3398..4372)
                     /locus_tag="VF516_17350"
     CDS             complement(3398..4372)
                     /locus_tag="VF516_17350"
                     /inference="COORDINATES: protein motif:HMM:NF008024.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEX8109503.1"
                     /translation="MRAIQIDRFGGADEMHLADVPVGDPGPGEVRVRHRACGINFIDI
                     YHRTGLYPNQLPLVLGCEGAGVVEAVGAGVTHLAVGDRVAYAARTPGSYAEVRVLSAI
                     PVVKLPDAIDFDTGAAMMLKGLTVRYLLRKTRVELAPGEPILWHAAAGGVGLIACQWA
                     KALGLRLIGTAGSDDKCKLAEQHGAAYTINYRREDVVARVREITGGKGVNVVYDPVGK
                     DTWERSLDCLAPLGVLVSFGNASGPVAPVNLGVLSLKGSLYVQRPTLGTHQATREAAQ
                     EGADDLFAMVASGKVKIPVERRYPLAEAAQSHRDLEGRGTTGAGVLVP"
     gene            4412..5344
                     /locus_tag="VF516_17355"
     CDS             4412..5344
                     /locus_tag="VF516_17355"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8109504.1"
                     /translation="MLVVVTGGAGFIGSHTVDRLLGEGHRVVVLDDFRTGKRANLAHH
                     AGSDRLDVVACDVSHGIFAALAPITARHGPVERIVHLAAQVSVVHSVQNPLVDMQVNY
                     GGTLHVLEYARATGVQKVVFASSAAVYGEVATLPVDEDTPTRPVSPYGIDKLASEHAL
                     DYYAAVHGVPATSLRFFNVYGPRQDPSSPYSGVISIFAERARAGRALTIFGDGGQTRD
                     FVYVGDVVRAIVAALGDGADRVIANVGTGGEITVLELARSIVELCGGRSAIEHAPPRA
                     GEILKSRARVDRLRDALGVVAETSLIDGLRETLR"
     gene            complement(5376..5825)
                     /locus_tag="VF516_17360"
     CDS             complement(5376..5825)
                     /locus_tag="VF516_17360"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8109505.1"
                     /translation="MRALARSRLGLGTALVALCAAAGCSRRADAPEQRPAAATMASVS
                     PVSVDELDHLLASRQCVAVDANGDSTRRQMGVIPGAVLLRDLDAVDQLPADRSTHLVF
                     YCTNSACRASHEAAEKAIAAGYRDVRVLPDGIAGWVKAGKRTAATSI"
     gene            5876..6067
                     /locus_tag="VF516_17365"
     CDS             5876..6067
                     /locus_tag="VF516_17365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109506.1"
                     /translation="MPRAVVAHLARALIWGTTWYAIRVSIAAYPTLALFERGLPLTGR
                     AYLGAAITLSGLAVSLRRR"
     gene            complement(6080..>6330)
                     /locus_tag="VF516_17370"
     CDS             complement(6080..>6330)
                     /locus_tag="VF516_17370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109507.1"
                     /translation="PIESTRRFFESELPSRVPSPRAALMFHCGGRKLYAEATGRLPEL
                     ASVFSAAPPSIGMHVLFEIYCGFTINTTLTTLAFGATP"
BASE COUNT          857 a         2237 c         2323 g          913 t
ORIGIN      
        1 gatcgacgag ggcgccgagg gcgtcgacct gatgccgacg gtgctgcagg cgatcggcgc
       61 gcccgcgtcg ccggcgcccc agggcgagcc gctggagccg ctggcccagg gcatcgggcg
      121 cggctgggcc cggccgtcgt acgcctcgca gtacgagtac gcgcacgcga tgcggatcgg
      181 ccgctggaag ctccgggtcg gcccgcgcgg cacgccgctg ctcggcgaca tggcggccga
      241 tccgggcgag acccgcgatg ccgcatcggg tcatcccgta gagcgccgga tgctgaccga
      301 caacctcggc ctgttcctcg cgctccgcac ccagtggcgc aaggccgagt ggggcgtggt
      361 gaccagtgtc agtcccgccg gggcgcgcgc gctcgatgga gcgttcacgc catgaccggc
      421 cgtgcgaggc cggaagcggt gctggcgtgc gcgatcggcg cagcgtgcgc ggcgtgcgcg
      481 gcatgcgcga cccaggacgt gtcgccggtc gcgtcgacct cgccgcggat cgccgatggg
      541 ccgagctcca gcccggcggg cgacgaggcg agaaacgagg cggtggacga gctggattcc
      601 cgcgagtcgt tcgagcccgg cgtgtcggtc gagtcggtcg agccgatccc gctcgggccc
      661 gccgatgtcc tgtcgccgga ggccgaggtc tggctcaagg gagtgaccca cgtccacgcc
      721 cgggcgtcgg gggattcgag cgagccgccg ggcaacgtga tcggctggta cgagcagcac
      781 ggctacgact tcatcgcgct gaccgaccac aaccgggtca gcgagctcga ccgcagcaac
      841 gacacccgcg gccaggtcac gctgcgcgat ccgcacgccg ggctgatcgt gttctccggg
      901 atcgagctca cccacaaccc gatcggctgc ctgccgatcg gcgatgccag ccggaactgc
      961 cgcatccacg tcaacctggt cggaccgacc gcccggccgg ccggccggat cgagtgggcg
     1021 gagcggcaca gccacctccg gatcgacatg taccaggccg cggtcgtggc ccagaccgcc
     1081 ctcggcggca tcgcccagat caaccacccg cagtggctgt ggggcatgac cccggatctc
     1141 ctggtcgagc tggcgcaccg cgggatgccg ctgatcgaga tcgccaacgc cgcgttctcc
     1201 aggtggaacg ccggggacgt ggcgcacccg tcgaccgagg tgctatggga cgcggcgctg
     1261 gcgcgcggcg cgacgctatg ggggatcgca tccgatgacg cccacgacta cgagggtcac
     1321 ggaaagtacc cacccggggg tggctgggtg atggtgcgcg cgcgccgcga tccccgggcg
     1381 atcctcgatg cgctggccgc cgggcggttc tacgcgtcga acggcgtggt gctggagcgc
     1441 gcggaggtcg agggcgacga gctggcgatc gccgtcgatc cggccgcccc gggcagctac
     1501 acgatcgact tcatcgagaa tggcaggcgg gtcgactcgg tccacggccg gttcgcgcgg
     1561 cgccgcgtgc cggcggccgg ctatgtccgt gctgtcgtga cccgcaacga tggcaagcgc
     1621 gcctgggttc agcccgcccg gcggtgagcc cgggcggcgt gcctgcccat gaggggaggg
     1681 ggatcgtcct agagcgccgt gtagccgtgc ttgaccgcga accgggtgag ctcggagttg
     1741 ttgcgcaacc cgagcttctt caggacatgg ccgcggtggg tgtcgacggt cttgatgctg
     1801 atctccaggt gctccgcgat ctcgcggttg gtcataccgc gcgccagcat ctccatgacc
     1861 tgctgctcgc gggccgtgag ggcgctggcg gggtggccgt cacccgtcct gagtaattct
     1921 tctaccccag gggggatcac ctgctcaccg cggctgaccg cgcggatcgc ggccaggagc
     1981 tcgtcgggct cgaccgcctt gttcacgtag ccgtgcgccc cggccgcgat ggctctcgct
     2041 gcgtagctga gctcggtgtg ccaggtgagc acgacgacca gcggcgccgt gtccatcggc
     2101 cgcatctcgg tgagaacgtc gaagccggag cgatccggca ggctgatgtc gaggatcagt
     2161 acatccggcc gctcgcgctt gatcatctcg agcgtttctg cggctgtgcc cgcttctccg
     2221 acgacttcga tgtcgtctgc cttgctgagc gcctctctca gcgcccagcg aacaatggtg
     2281 tggtcttctg cgatcgccag tcttatcatc gggacctccg cggcctgtgt gcgaaaatta
     2341 agcaaccccc gcgcctacct ggggtacgcc tgggtgaggc cgatctgtcc acgtgttgca
     2401 tgaaagctgc actctgagat ctcttcgtcg gtgaggtgcc gagccgcgcg gcgacggccg
     2461 agatcggtcg cgcttcgtga tccgcgctac cggcctcggg ggccgtcgag caactccttc
     2521 tcacagctta gtggcatccg gcggcaagcg cacgtagttc ctcgcgttga tgcaacgcgc
     2581 gtacgcggat cccggtacgc cggcgaccgc tgcgctcggg ggaggccggg cgcccggccc
     2641 tgcccggacg gcctgtccgg gacccgtaca gccgtgcaag gcctgcgcgg cgggcgcggt
     2701 cgcgcgctgg cgcgcgagaa attcggggag tgcagcgcgt ggggggtgca gcgcgtgggg
     2761 ataggatggc cgcgatgttg cgccgcccct acgtcgtcgg gatcgccgga ggcaccggct
     2821 cgggcaagac cacggtcgcc gacaagctcg ccgccgcgat gccgcccgga cgctgcgtca
     2881 cgctcgatca cgacgcgtac taccgcgatc aggggcacct gccgccggcg gaccgcgagg
     2941 ccatcaacta cgaccacccg tcggcgctcg acagcgcgct gctggcgctc cacctgcgcg
     3001 agctgcgcga cgggcgcacc gtcgaggtgc cgatctacga cttcgcgacc catacccggc
     3061 gccgcgacac ccggaccgtg gcgccggcgc gggtgatcct cgtcgagggc atcctggtgt
     3121 tcgccgagcc cgcgctgcgc gagcagatgg acatcaagat cttcgtcgac accgacgccg
     3181 acatccggct gatgcgacgg atccgccgcg acctggagga gcgcggccgg acgttccagt
     3241 cggtgcgcga ccagtaccac gcgaccgtgc gcccgatgca cctggagtac gtcgagccga
     3301 gcaagcggtg ggccgacctg atcctccccg agggcgggga caaccgggtc gcgctcgacg
     3361 tgctgctcgg ccagctgtgg cgcgttgtcg cggagcccta gggcaccagg acgccggcgc
     3421 ccgtcgtgcc gcggccctcg aggtcgcggt gggactgggc cgcctcggcg agtgggtagc
     3481 gccgctcgac cgggatcttg accttgccgc tggcgaccat cgcgaacagg tcgtcggccc
     3541 cctcctgcgc ggcctcgcgg gtcgcctggt gggtgcccag cgtgggccgc tggacgtaca
     3601 gcgagccctt gagcgacagc acgccgaggt tgaccggcgc caccgggccc gaggcgttgc
     3661 cgaagctgac cagcacaccg agcggcgcga ggcaatccag cgagcgctcc caggtgtcct
     3721 tgccgaccgg gtcgtagacg acgttgacgc ccttgccgcc ggtgatctcg cggacccggg
     3781 cgacgacgtc ctcgcggcgg tagttgatgg tgtaggccgc gccgtgctgc tcggcgagct
     3841 tgcacttgtc gtcgctgccc gcggtgccga tcaggcgcag cccgagcgcc ttcgcccact
     3901 ggcacgcgat caggccgacg ccgccggccg ccgcgtgcca caggatcggc tcgccggggg
     3961 ccagctcgac ccgggtcttg cgcagcaggt accgcaccgt gagccccttg agcatcatcg
     4021 cggcgccggt gtcgaagtcg atcgcgtcgg gcagcttgac gaccgggatc gcggacagga
     4081 cgcggacctc ggcgtagctg cccggggtcc gcgcggcgta ggcgacccga tcgccgaccg
     4141 cgaggtgcgt gacgccggct ccgaccgcct cgaccacgcc ggcgccctcg cagccgagca
     4201 cgagcggtag ctggttcggg tacagcccgg tgcggtggta gatgtcgatg aagttgatgc
     4261 cgcacgcccg gtgcctcacg cggacctccc cggggccggg gtcgcccacg gggacgtcgg
     4321 ccaggtgcat ctcgtccgcg ccaccgaatc ggtcgatctg gatcgctctc atggtggccg
     4381 gaacctacca ccggcggggt atagtggatc catgctcgtg gtcgtaaccg ggggcgccgg
     4441 cttcatcggc tcgcacaccg tcgatcggct gctcggcgag ggccaccggg tcgtcgtgct
     4501 cgacgacttc cgcaccggca agcgcgccaa cctcgcgcac cacgccggga gtgaccggct
     4561 cgacgtggtg gcgtgcgacg tgtcgcacgg catcttcgcc gcgctggccc cgatcaccgc
     4621 gcggcacggg ccggtcgagc gcatcgtcca cctcgccgcg caggtctcgg tcgtgcactc
     4681 ggtgcagaac ccgctggtcg acatgcaggt caactacggc ggcacgctgc acgtcctcga
     4741 gtacgcgcgc gccaccggcg tccagaaggt cgtgttcgcc tcgtcggccg cggtctacgg
     4801 cgaggtcgcg accctgccgg tcgacgagga caccccgacc cgcccggtgt cgccctacgg
     4861 gatcgacaag ctcgcctccg agcacgcgct cgactactac gcggccgtcc acggcgttcc
     4921 ggcgacctcg ctgcggttct tcaacgtcta cggcccgcgc caggacccgt cgagcccgta
     4981 ctccggcgtg atctcgatct tcgccgagcg cgcccgggcc gggcgggcgc tgacgatctt
     5041 cggcgacggc ggccagaccc gcgacttcgt ctacgtcggc gacgtcgtgc gcgcgatcgt
     5101 cgcggcgctc ggcgatggcg ccgaccgcgt gatcgccaac gtcggcaccg gcggcgagat
     5161 caccgtgctc gagctcgcgc gctcgatcgt cgagctgtgc ggcgggcgga gcgcgatcga
     5221 gcatgcgccg ccgcgcgccg gcgagatcct caagtcgcgc gcgcgggtcg accggctgcg
     5281 cgatgcgctc ggcgtggtcg ccgagacctc gctgatcgac ggcctgcgcg agacgctgcg
     5341 ctagccgtgg ggcgtggcga gcaggagagc tcggactaga tcgacgtcgc cgcggttcgc
     5401 ttgccggcct tgacccagcc ggcgatgccg tcggggagca cgcggacgtc gcggtagccc
     5461 gcggcgatcg ccttctcggc ggcctcgtgc gaggcccggc acgcggagtt ggtgcagtag
     5521 aacaccaggt gggtggaccg gtcggccggc agctggtcga ccgcgtcgag gtcgcggagc
     5581 agcacggcgc cggggatcac gcccatctgg cgccgcgtcg agtcgccgtt ggcgtcgacc
     5641 gccacgcact ggcggctcgc cagcaggtgg tcgagctcgt cgaccgagac cggcgagacc
     5701 gacgccatgg ttgcggccgc gggacgctgc tcgggggcgt cggcgcgccg ggagcagccg
     5761 gcggccgcgc agagcgcaac gagcgcggtg cccagcccga gccgagatct cgccagcgcg
     5821 cgcatgaccg gaccgtaccc gagcgcccat gccccggcaa tgcgacactg cgccggtgcc
     5881 tcgcgccgtg gtcgctcacc tcgcgcgcgc gctgatctgg ggaacgacct ggtacgcgat
     5941 ccgggtctcg atcgctgcat atcccacgct cgcgctgttc gagcgcgggc tgccgctcac
     6001 cgggcgtgcc tacctcggcg ccgcgatcac gctgagcggg ctcgccgtga gcctgcgccg
     6061 gcgatgaccg gggcggtgat cacggggtcg cgccgaacgc cagcgtggtg agcgtggtgt
     6121 tgatcgtgaa gccgcagtag atctcgaaca gcacgtgcat gccgatgctg ggcggggcgg
     6181 cgctgaacac gctggccagc tcgggcaggc ggccggtggc ctcggcgtac agcttgcggc
     6241 cgccgcagtg gaacatcagc gcggcgcgcg gcgacgggac ccgcgacggc agctcggact
     6301 cgaagaaccg ccgcgtgctc tcgatggggt
//
LOCUS       DASVOR010000892         5662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447124,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000892 DASVOR010000000
VERSION     DASVOR010000892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5662
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447124"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..982
                     /locus_tag="VF516_17375"
     CDS             <1..982
                     /locus_tag="VF516_17375"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8109508.1"
                     /translation="HKAFFSTGGSEANEVALKMARQFRKKPEIAYLENGYHGLTLGSL
                     EVTANQKYRESAGKPLGTDNYAVPTAYCYRCKYGPHTQCKVECLDGIDALFDAHPGTA
                     GLIAEPIQAVGGLAPPQKWWDRLDAARKKRGIVLIADEIQTGMGRTGKMFAIDHYGLS
                     PEILTGGKGLSGGVGSLGLTAASNEIATTFFGATTPTNGGNAVSAAGGIAMIETIFAE
                     GLIENAARMGRYFTEAAWALRDPWIGDVRFTGLLGGIELVKNRDTREVLGKTEMGKVK
                     DHLHAQGMLITVSGLHGNVLRLQPPLSITAQQLDQFIAALRNTLRTVRGT"
     gene            1058..1783
                     /locus_tag="VF516_17380"
     CDS             1058..1783
                     /locus_tag="VF516_17380"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8109509.1"
                     /translation="MNLFAIRKYVDRLLHPVVASLARLPLHANAWTMFGALVGLVGGV
                     VFFYGYWGWGFALLLVRGLIDHIDGYKARNFNQRSTFGAVMDDVCDRWVLGIMYAGGC
                     LNLSYDYPHVLIVLGFGITGSLTNVIIKLSIYAESQQDIWREKGKIGHPVDVVGLFGS
                     AEFLIYYGFGVLFTALLHDPRPALAGCWAVAIMSHVSLLQRVRFAWKRYRFVDPGLAA
                     GVDPAGQPDSPAPAGIMTDAEAS"
     gene            1783..2493
                     /locus_tag="VF516_17385"
     CDS             1783..2493
                     /locus_tag="VF516_17385"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8109510.1"
                     /translation="MAAASSKAAGKPAGKPAGPRVVSPWNPANAVTASRFLFLPPLWW
                     SVAHGYHQWATLFIILCGLIDKLDGLVARIFDCRSAFGELFDAITDGLCYGFGLILVA
                     AYGWAPVIPVGCVIGLGLLNTAMRAVYIRRAGRATNYKSYAMERIVGYTGFLIGFATS
                     GMEVTFFYWAFVPLMLIIVLHDAKRMLIDPVPGPAPGPAVEPVEQAAGWSAAPATEPS
                     RSVRRSGPVTGAAAEVRP"
     gene            2490..2720
                     /locus_tag="VF516_17390"
     CDS             2490..2720
                     /locus_tag="VF516_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109511.1"
                     /translation="MTELPDGVFHVLISLFTGGLAGAWLIHDLVLLARVVRRRGTGRR
                     DPRVGDQVFGCVMGIVIGVVGLVGTLRYNGVF"
     gene            complement(2717..3466)
                     /locus_tag="VF516_17395"
     CDS             complement(2717..3466)
                     /locus_tag="VF516_17395"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8109512.1"
                     /translation="MTWLGHATAVAELGRARVVFDPLLPWRARRAGRVDAVLVTHSHI
                     DHLNRWSLKAIDRDTHLVVPRGARHIVADLGFRQLTELTAGDALEIAGLAITAVPTRH
                     DFGRWRKGGSPEALGYVVAGGGAALHHAGDVDFSDHAIFDDIGKRHAIDAALLPIGGM
                     LPTWYYRWRRRVFDRGIHIDPDCALDIFERLGARTLVPVHWGTVHVLGLPGAPRRRLA
                     QVAGQRGTTARVRVLAHGERLELAQASSQEH"
     gene            3607..4242
                     /locus_tag="VF516_17400"
     CDS             3607..4242
                     /locus_tag="VF516_17400"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX8109513.1"
                     /translation="MFILTGVAAGALTGVPIGPVNVAVIDAAYRHTLRRAIAVGLGGA
                     CADFLYAALGILGVTPVLRSYAIVSPILYAVSGIVLLVYGFLTARSQPVTPTHHDALV
                     PAVPENELVLGFKTGLLLILLNPAAVVTWVVITEQIADHLGSPSTLTGLLCAVGVMGG
                     SFAWFALVAHLTQRGKNVLGDKAAWIPRVIGVALMVYAVYLIARAVKYAVT"
     gene            complement(4223..4495)
                     /locus_tag="VF516_17405"
     CDS             complement(4223..4495)
                     /locus_tag="VF516_17405"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX8109514.1"
                     /translation="MARRSWKDVLRPGLDVVFVGYNPSRLAWRTGHHYANPGNRFYHL
                     LHDAGLTPRRFAPAECRDLPGLGIGLADLLPTPARPVRAVTTSRRT"
     gene            complement(4589..>5662)
                     /locus_tag="VF516_17410"
     CDS             complement(4589..>5662)
                     /locus_tag="VF516_17410"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8109515.1"
                     /translation="PRASGAPIAPPGEHRLASREALVFWVVGALVLGVVVVLHVAADV
                     LLLTFAGLLFGNALRGAAEWLSRRTRSGVGQSLVVCILGLLALGAGAVLWVAPLVADQ
                     AGQLESQLGAAIADLQAWLSRTQLVHALSTAQPGGLERQATGWLTEHAGFAGSFLLGV
                     GGVLGAVVYVLFVTLYFAFSPAVYRRGMLALVPPGQRARAEQLLDALTTTLRRWLGAQ
                     LITMVALAIASGIGLRLLGIPLAFALGLLAGVLLFVPYLGSIASAIPALLIALTVSPM
                     HVLYVALLYLGIHLAEGYLLSPLLQRRAVDTPALLVLGSQLIAGALWGILGLMFATPI
                     VATVLVVVRMLYVEDTLGSDQGR"
BASE COUNT          822 a         1960 c         2061 g          819 t
ORIGIN      
        1 ccacaaggcg ttcttctcga cgggcggctc ggaggccaac gaggtcgcgc tcaagatggc
       61 gcggcagttc cgcaagaagc ccgagatcgc gtacctcgag aacggctacc acggcctgac
      121 cctgggctcg ctcgaggtca cggcgaacca gaagtaccgc gagtcggcgg gcaagccgct
      181 cggcaccgac aactacgcgg tcccgacggc gtactgctac cgctgcaagt acgggccgca
      241 cacccagtgc aaggtcgagt gcctcgacgg catcgacgcg ctgttcgacg cccacccggg
      301 caccgccggg ctgatcgccg agccgatcca ggcggtcggc gggctggccc cgccgcagaa
      361 gtggtgggac cggctcgacg cggcccgcaa gaagcgcggg atcgtgctga tcgccgacga
      421 gatccagacc ggcatgggcc gcaccggcaa gatgttcgcg atcgatcatt acggcctttc
      481 gcccgagatc ctcacgggcg gcaaggggct gtccggcggg gtcggctcgc tcgggctgac
      541 cgcggcgtcc aacgagatcg cgacgacgtt cttcggcgcc accacgccga ccaacggcgg
      601 caacgcggtg tcggcggccg gcgggatcgc gatgatcgag acgatcttcg ccgagggcct
      661 gatcgagaac gcggcccgga tgggtcgtta cttcaccgag gcggcgtggg cgctccgaga
      721 tccctggatc ggcgatgtcc ggttcaccgg cctgctcggg ggcatcgagc tggtcaagaa
      781 ccgcgacacc cgcgaagtgc tcggaaagac cgagatgggt aaggtgaagg accatcttca
      841 cgcccagggg atgctgatca cggtctcggg cttgcacggg aacgtgctgc ggttgcagcc
      901 tccgctatcg atcacggcgc aacagctcga tcagttcatc gccgccctgc gaaatacatt
      961 gcgcaccgtc cgggggacgt gatcgatcgt atggttggcg agccgtcggt ggactgtccg
     1021 gccggcggca acttcgatta gagtccctcg gtccccgatg aacctgttcg cgatccgcaa
     1081 gtacgtcgac cggctgctgc acccggtcgt ggcctcgctc gcccggctgc cgctgcacgc
     1141 caatgcctgg acgatgttcg gggcgctggt cgggctggtc ggcggcgtgg tgttcttcta
     1201 cggctactgg gggtgggggt tcgcgctgtt gctggtgcgt gggctgatcg atcacatcga
     1261 cggctacaag gcgcgcaact tcaaccagcg ctcgacgttc ggcgcggtga tggacgacgt
     1321 ctgcgaccgc tgggtgctcg ggatcatgta cgccggcggc tgcctgaacc tcagctacga
     1381 ctacccgcac gtcctgatcg tgctcggatt cggcatcacc ggctcgctga ccaacgtcat
     1441 catcaagctg tcgatctacg ccgagagcca gcaggacatc tggcgcgaga agggcaagat
     1501 cggccacccg gtcgacgtcg tcgggctgtt cggctcggcg gagttcctga tctactacgg
     1561 gttcggcgtg ctgttcaccg cgctgctcca cgacccgcgg cccgccctcg ccgggtgctg
     1621 ggcggtcgcg atcatgagcc acgtgtcgct gctccagcgc gtgcggttcg cgtggaagcg
     1681 ctaccgcttc gtcgatcccg ggctcgccgc cggcgtggat ccggcgggcc agccggattc
     1741 gccggcgccg gcgggcatca tgaccgacgc cgaggcgtcg tgatggcagc cgcttcatcc
     1801 aaggcagccg gcaagccagc cggcaagcca gccggcccgc gggtggtgtc gccgtggaac
     1861 ccggccaacg cggtcaccgc gtcgcggttc ctgttcctgc cgccgctgtg gtggtcggtc
     1921 gcgcacggct accaccagtg ggcgacgctg ttcatcatcc tgtgcgggct gatcgacaag
     1981 ctcgacggcc tggtggcccg gatcttcgac tgccgcagcg cgttcggcga gctgttcgac
     2041 gcgatcaccg acgggctgtg ctacggcttc gggctgatcc tggtggcggc ctacggctgg
     2101 gcgccggtga tcccggtcgg ctgcgtgatc ggcctgggcc tgctcaacac ggcgatgcgt
     2161 gcggtctaca tccggcgcgc cggccgggcg accaactaca agtcgtacgc gatggagcgg
     2221 atcgtcggct ataccggctt cctgatcggg ttcgcgacca gcggcatgga ggtcaccttc
     2281 ttctactggg cgttcgtgcc gctgatgctg atcatcgtgc tgcacgacgc caagcgcatg
     2341 ctgatcgatc cggtgccggg gcccgcgccc gggccggcgg tggagccggt cgagcaagcc
     2401 gccggatggt cggccgcgcc ggccaccgag ccgtcgcggt cggtccgcag gtccgggccc
     2461 gtgaccgggg ccgccgcgga ggtccggcca tgaccgagct gcccgacggt gtgttccacg
     2521 tgctgatcag cctgttcacc ggcgggttgg cgggcgcgtg gctgatccac gatctcgtgc
     2581 tcctcgcgcg ggtcgtccgg cgccgcggca ccggacgccg cgatccgcgg gtcggcgacc
     2641 aggtgttcgg ctgcgtgatg gggatcgtga tcggcgtggt cggcctggtc gggacgctgc
     2701 ggtacaacgg cgtgttctag tgctcctgcg acgacgcttg cgccagctcc aggcgctcgc
     2761 catgggcgag cacgcgcacc cgagcggtcg tgccgcgctg cccggcgacc tgcgccagcc
     2821 ggcggcgcgg cgcgccgggc aggccgagca cgtggacggt cccccagtgc accgggacca
     2881 gcgttcgcgc gcccaggcgc tcgaagatgt cgagcgcgca gtcgggatcg atgtggatcc
     2941 cgcgatcgaa cacgcggcgg cgccagcggt agtaccaggt cggcagcatg ccgccgatcg
     3001 gcagcagcgc ggcgtcgatc gcgtggcgct tgccgatgtc gtcgaagatc gcgtgatcgg
     3061 agaagtcgac gtcgcccgcg tgatggagcg cggcgccgcc gccggcgacc acgtagccca
     3121 gcgcctcggg cgagccgccc ttgcgccagc gcccgaagtc gtggcgggtc ggcaccgccg
     3181 tgatcgcgag cccggcgatc tcgagcgcgt cgccggcggt cagctcggtc agctggcgaa
     3241 acccgaggtc ggcgacgatg tgcctggcgc cgcgcggcac gaccaggtgg gtgtcgcgat
     3301 cgatcgcctt gaggctccag cggttcaggt ggtcgatgtg cgagtgcgtg accagcaccg
     3361 cgtcgacccg gccggcgcgg cgggcgcgcc acggcagcag cggatcgaac accacgcggg
     3421 cgcggccgag ctcggcgacc gcggtggcgt ggccgagcca ggtcacggtc gggccaccgg
     3481 cgctcggcgc gtgctgcaat gcggcagagg agcccagcgg ttccggccgg tccagcgccc
     3541 ttggcgagcg cggctttctg gcagctgggc ccacggtgct gctatagcac ggaggcttcc
     3601 gtgctgatgt tcatcctgac cggggttgct gcgggcgcgc tcaccggagt gccgatcggc
     3661 ccggtcaacg tcgcggtgat cgacgccgcc tatcgccaca cgctgcggcg cgcgatcgcg
     3721 gtcggcctcg gcggcgcgtg cgccgacttc ctgtacgcgg cgctcggcat cctcggcgtc
     3781 acgccggtgc tgcggtcgta cgcgatcgtc tcgccgatcc tctacgccgt gagcggcatc
     3841 gtgctcctgg tctacggctt cttgaccgcg cgcagccagc cggtgacccc gacccaccac
     3901 gacgcgctgg tgcccgcggt gccggagaac gagctggtgc tcggcttcaa gaccggcctg
     3961 ctgctgatcc tgctcaatcc cgccgcggtc gtgacctggg tcgtgatcac cgagcagatc
     4021 gcggaccacc tgggcagccc gtcgacgctg accggccttt tgtgcgcggt cggtgtgatg
     4081 ggcggcagct tcgcgtggtt cgccctggtc gctcacctca cccagcgcgg caagaacgtg
     4141 ctcggcgaca aggccgcctg gatcccgcgc gtgatcggcg tcgcgctgat ggtgtatgcg
     4201 gtctacctga tcgcgcgggc cgtcaagtac gccgtgacgt agtcactgcc cggaccgggc
     4261 gggccggggt agggagcagg tcggccaggc cgatgccgag gccgggcagg tcgcggcact
     4321 cggcgggtgc gaagcggcgc ggcgtgagcc cggcgtcgtg gagcaggtga tagaaccggt
     4381 tgcccgggtt ggcgtagtgg tgcccggtgc gccacgccag gcgcgacggg ttgtagccga
     4441 cgaacacgac gtcgaggccg ggccggagca cgtctttcca ggagcggcga gccaccggcg
     4501 gagtatagcg agcgcagccc gcggcctccg ggaacccttc gaggcgctat cgcgaggcgc
     4561 gtgtgcgcgc tctcctgggc gccatgccct accgaccctg gtcggacccg agcgtgtcct
     4621 cgacgtacag catccggacc acgaccagca cggtcgccac gatcggggtc gcgaacatca
     4681 ggccgaggat gccccacagc gcgccggcga tcagctggct gccgagcacg agcagcgccg
     4741 gcgtatcgac cgcgcggcgc tgcagcagcg gcgacagcag gtagccctcg gccaggtgga
     4801 tgccgaggta cagcagcgcg acgtagagca cgtgcatcgg actgacggtg agcgcgatca
     4861 gcagcgccgg gatcgccgac gcgatcgagc cgaggtacgg cacgaacagc agcacgccgg
     4921 cgagcaggcc gagcgcgaag gcgagcggga tgccgagcag gcgcagcccg atccccgatg
     4981 cgatcgcgag cgcgaccatc gtgatcagct gcgccccgag ccagcgccgc agcgtcgtgg
     5041 tcagcgcatc gagcagctgc tcggcgcgcg cgcgctgccc gggtgggacg agcgcgagca
     5101 tgcctcgccg gtagaccgcc ggtgagaacg cgaagtacag cgtcacgaac agcacgtaca
     5161 ccaccgcacc gagcaccccg ccgacgccga gcaggaagct cccggcgaac ccggcgtgct
     5221 cggtcagcca gccggtcgcc tgccgctcga gcccgcccgg ctgcgcggtg gacagcgcgt
     5281 ggacgagctg cgtcctgctc agccaggcct gcaggtccgc gatcgccgcg ccgagctggc
     5341 tctcgagctg cccggcctgg tcggcgacca gcggggcaac ccacagcacc gcgccggcgc
     5401 ccagcgcgag caagcccagg atgcacacca ccaggctctg cccgaccccg gacctggtcc
     5461 ggcgcgacaa ccactcggcc gcgccgcgca gcgcgttgcc gaacagcagg ccggcgaacg
     5521 tcagcagcag gacgtccgcg gcgacgtgca gcacgaccac gacgccgagc accagcgcgc
     5581 cgaccaccca gaacacgagc gcctcgcggg aggcaaggcg atgctcgccg ggcggagcga
     5641 tcggcgctcc ggatgcccgg gg
//
LOCUS       DASVOR010000893         2611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_330351,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000893 DASVOR010000000
VERSION     DASVOR010000893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_330351"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="VF516_17415"
     CDS             <1..324
                     /locus_tag="VF516_17415"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEX8109516.1"
                     /translation="GIELHALVGRGNPLGAGSPGARVQRRPLQAGDLVVWYTDGVVEA
                     QDPSGAPFGDRRLQHLLKKLDRRRLTPPAVHDLVLRGVAVHRAGRPLADDETLVVAQV
                     REAAP"
     gene            321..1097
                     /locus_tag="VF516_17420"
     CDS             321..1097
                     /locus_tag="VF516_17420"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8109517.1"
                     /translation="MRLRTLSYNIHKCIGGVDRRYDPSRIVEVIQKLDVDVAMLQEVD
                     GGVARSNHDHQVDLLAERLGLRYRTWFPNVDVRGGGQYGNAILSRYPIIESTNLDLTI
                     RFKKRRSALHAVLRVRHDGVDRTVHVYNMHLGLARYERRRQLRMFLDSHPFQNLHHDT
                     PIVVGGDLNDVYGGLGELLAPAGFRGIERRPLTFPAWGPMRALDAIFVRGAMDFLQLS
                     RCDSELARRASDHRPLVAEVRLQAHPHPEHGHHKPPAPRP"
     gene            complement(1106..1279)
                     /locus_tag="VF516_17425"
     CDS             complement(1106..1279)
                     /locus_tag="VF516_17425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109518.1"
                     /translation="MPTAADEPLIRRQVFELIHLEVPGVPVLSRDEIAPGTRPRILHR
                     IDALREAGRASAS"
     gene            complement(1410..1730)
                     /locus_tag="VF516_17430"
     CDS             complement(1410..1730)
                     /locus_tag="VF516_17430"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8109519.1"
                     /translation="MSDLGAMAIRFDDEADAELLGAGQYYAERSPKAAADFAAAVEAA
                     LVQVQQMPRSAPAWPGRPDVRRRVLAKFPYAIVYMVVEPTEIIVVAVEHVKRRPGYWL
                     GRVR"
     gene            complement(1798..2043)
                     /locus_tag="VF516_17435"
     CDS             complement(1798..2043)
                     /locus_tag="VF516_17435"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX8109520.1"
                     /translation="MAIEAVLDEALRLPEEERAELVSRLLDSLEDSEVVEPGHVAAWT
                     EVIDRRVERIREGRAKLIDADVTMARLRAVVASSRLR"
     gene            complement(2082..>2611)
                     /locus_tag="VF516_17440"
     CDS             complement(2082..>2611)
                     /locus_tag="VF516_17440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8109521.1"
                     /translation="KSGETVQLGYVDQSRDALDADKSIYDEISEGAEHLQLGERSVNA
                     RAYVSSFNFKGQDQQKKVGILSGGERNRVHLAKMIKRAGNVLLLDEPTNDLDVDTLRA
                     LEEALLNYAGCAVVISHDRWFLDRIATHILAFEGDSHVEWFEGNFADYEADRHRRLGT
                     EADQPHRIKYKPLRA"
BASE COUNT          408 a          956 c          837 g          410 t
ORIGIN      
        1 gggatcgagc tccacgcgct ggtcgggcgc ggcaacccgc tgggcgccgg ctcgcccggc
       61 gcccgggtcc agcgccggcc gctccaggcc ggtgatctcg tggtctggta taccgacggc
      121 gtggtcgagg cccaggatcc atccggcgcg ccgttcggcg atcgccggct gcagcacctg
      181 ctcaagaagc tcgatcgccg gcggctgacc ccgcccgcgg tccacgacct cgtgctgcgc
      241 ggtgtcgccg tccaccgcgc cggccggccg ctcgccgacg acgagaccct ggtcgtcgcg
      301 caggtccgcg aggccgcacc gtgaggctgc gcacgctcag ctacaacatc cacaagtgca
      361 tcggcggggt cgaccggcgc tacgacccca gccggatcgt cgaggtgatc cagaagctcg
      421 acgtcgacgt cgcgatgctg caggaggtcg acggcggggt cgcgcggtcc aaccacgacc
      481 accaggtcga tctgctcgcc gagcggctcg gcctgcgcta ccgcacctgg ttccccaacg
      541 tcgacgtccg cggcggcggc cagtacggca acgcgatcct gtcgcgctac ccgatcatcg
      601 agagcacgaa cctcgacctg acgatccggt tcaagaagcg gcgcagcgcc ttgcacgccg
      661 tgctccgggt ccgccacgac ggcgtcgatc gcacggtcca cgtctacaac atgcacctcg
      721 gcctcgcgcg ctacgagcgg cggcgccagc tccgcatgtt cctcgacagc catccgttcc
      781 agaacctcca ccacgacacc ccgatcgtcg tcggcggcga cctcaacgac gtgtacggcg
      841 ggctcggcga gctgctcgcg ccggcggggt tccgcggcat cgagcgccgg ccgctgacgt
      901 tccctgcctg ggggccgatg cgcgcgctcg acgcgatctt cgtgcgcggc gcgatggact
      961 tcctgcagct gtcgcgctgc gacagcgagc tcgcgcggcg cgccagcgat caccgcccgc
     1021 tggtcgccga ggtgcggctg caagcgcacc cccaccccga gcacggacac cacaagccgc
     1081 ccgcgccgcg cccgtagcta gctaactaac tcgcactggc gcgcccggcc tcccgaagtg
     1141 catcgatgcg gtggaggatc cgagggcgcg tcccgggcgc gatctcgtcg cgcgacagca
     1201 cgggcacgcc cggcacctcc aggtgaatga gctcgaacac ctgacggcgg atcaggggct
     1261 cgtcagcagc agtcggcatg aagcccgcgt cgatgacagc acgtggtcga gcggctcgcg
     1321 gcgtgcactg ccgaagaagc gttccgcgac cgcgtccata ttcggcgccc ggaccgcggc
     1381 cgtagcccgc cggtcacgcc aggcgcgcgt caccgaaccc gaccaagcca gtaaccgggg
     1441 cgtcgtttca catgttccac ggcaaccaca atgatctctg ttggttccac caccatgtac
     1501 acgatcgcat agggaaactt ggccagcact cggcggcgca cgtccggccg ccctggccac
     1561 gccggcgcag accgtggcat ctgctggacc tgcacgagcg cagcctccac cgcggcagcg
     1621 aagtccgcag cagccttcgg cgaccgctcc gcgtagtact ggcctgcgcc cagcagctcc
     1681 gcatccgcct catcgtcaaa ccgtatcgcc atggcgccga gatcgctcat ttgtcaagct
     1741 aaacgtcggc gacgtggccg tcgctgcaac tatgcggcag ttttcccaaa cggtcgatca
     1801 acgtagccga gacgaagcta cgacggctcg caaccgcgcc atcgtgacat ccgcgtcgat
     1861 cagcttcgcg cgaccttcgc ggatcctttc cacgcggcgg tcgatcacct cggtccaggc
     1921 agccacatgg ccaggttcaa ccacttcgct gtcttccaag ctatcgagta accgcgaaac
     1981 gagctcggca cgctcttcct ccggcagtct gagcgcctca tcgagcacgg cttcgatcgc
     2041 cacgggacca gtgtaccgca tttctccgcc gccacaaata gttacgcgcg cagcggcttg
     2101 tacttgatcc ggtgcggctg gtcggcctcg gtgccgagcc ggcggtggcg atcggcctcg
     2161 tagtccgcga agttgccctc gaaccactcg acgtgcgagt cgccctcgaa cgcgaggatg
     2221 tgggtcgcga tccggtcgag gaaccagcgg tcgtggctga tcaccacggc acaacctgcg
     2281 tagttgagca gcgcttcctc gagcgcacgc agcgtgtcca cgtcgaggtc gttcgtcggt
     2341 tcgtcgagca gcagcacgtt gccggcgcgc ttgatcatct tggcgaggtg gacgcggttg
     2401 cgctcgccgc ccgacaggat gccgaccttc ttctgctggt cctggccctt gaagttgaag
     2461 ctcgacacgt aggcccgggc gttgaccgag cgctcgccga gctgcaggtg ctcggcgccc
     2521 tcgctgatct cgtcgtagat cgacttgtcg gcgtcgagcg cgtcgcggct ctggtcgacg
     2581 tagccgagct ggacggtctc gccgctcttg a
//
LOCUS       DASVOR010000894         2205 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_535067,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000894 DASVOR010000000
VERSION     DASVOR010000894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535067"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VF516_17445"
     CDS             complement(<1..1039)
                     /locus_tag="VF516_17445"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109522.1"
                     /translation="MTGLIGRGGMGAVYAAEHALLGRPAAIKVLLPELSHKQDIVMRF
                     FNEARAATAIRHPGIIEIYDFGWTPSGAAFIVMERLEGETLGQRSSRVRLRWPAVLSI
                     ARQIAGALAAAHAQGIVHRDLKPDNVFLVPDPEVPGGERIKLLDFGIAKLADSSLSNM
                     TSAGTLMGTPTYMAPEQCRGVAIDHRVDLYALGCVIYELCCGRPPFVGEGTGDVLAAH
                     IHLPVPPMAALSPDIPPAVERLVQHLLAKAPAERMPSAEELIRAIDADTTVRDSATTG
                     PRRMVPVRTSPSVTTLSGAAAMSVPVTPGGRRRWPIAALSVSLAAITIVTVTLAMRRR
                     DDARTLAPPPVE"
     gene            complement(1488..>2205)
                     /locus_tag="VF516_17450"
     CDS             complement(1488..>2205)
                     /locus_tag="VF516_17450"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2891 family protein"
                     /protein_id="HEX8109523.1"
                     /translation="AYFAAPNRASYERPYGWAWLLQLAAELHTWDDADGRRWAAHLQP
                     LTDLLVARYLAFFPRQTYPIRTGVHSSTAFGFALARDHARAVGHRELEALIVERSAAY
                     YAADRDAPAAWEPSGDEFLSPSLIEADLMRRVLPPAEFPAWLHAFLPRGLPPSLQQPA
                     IVTDRSDGKLAHLDGLNLSRAWCLRAIAAALPAGDPLRSQLTASADAHAAAGLAHVAT
                     GDYAGEHWLASFAVYLLGVG"
BASE COUNT          333 a          758 c          825 g          289 t
ORIGIN      
        1 gttcgacagg gggcggggcg agcgtgcgcg cgtcatctct ccgtcgcatc gcgagcgtca
       61 cggtcacgat ggtgatcgcg gccaggctca ccgagagcgc ggcgatcggc caacgccgcc
      121 gcccacccgg cgtcaccggc acgctcatcg ccgccgctcc cgacagcgtg gtgaccgatg
      181 gcgaggtcct caccggaacc atgcgacgcg ggcccgtcgt ggcggagtcc cgaacggtgg
      241 tatccgcgtc gatcgctcgg atcagctcct cggcgctcgg catacgctcg gcgggggcct
      301 tggccaggag gtgctggacc agccgctcca ccgcgggcgg aatgtccggg cttagcgccg
      361 ccatcggtgg caccggcaaa tggatgtggg cggcgagaac gtcgcccgtg ccctcgccga
      421 cgaacggcgg ccggccgcag cacagctcgt agatcacgca gccgagcgcg tagagatcca
      481 cgcggtgatc gatcgcgacc ccgcggcact gctcgggcgc catgtaggtc ggcgtcccca
      541 tcagcgtccc ggcgctggtc atgttcgaca acgacgagtc cgcgagcttg gcgatcccga
      601 agtcgagcag cttgatccgc tcgccgcccg gcacctcggg atcggggacc aggaacacgt
      661 tgtccggctt gagatcgcgg tggacgatgc cctgggcgtg cgcggcggcc agcgcgccgg
      721 cgatctgccg cgcgatcgac agcacggcgg gccagcggag ccgcacccgc gacgatcgct
      781 ggccgagcgt ctcgccctcc aggcgctcca tgacgatgaa cgccgcgccg ctgggggtcc
      841 agccgaagtc gtagatctcg atgataccgg ggtggcgaat cgcggtcgcc gcgcgcgctt
      901 cgttgaagaa ccgcatcacg atgtcctgct tgtgcgacag ctccggcagc agcaccttga
      961 tcgccgccgg acggccgagc agcgcatgct cggcggcgta caccgcgccc atcccacccc
     1021 ggccgatcag accagtcacg cggtactgcc cgatcgtcgt gccgtccatc aaacgggctc
     1081 gatagtacag caacgatccg gagcgacaat catggagtgg atgccctcgc cgcgtcgtcc
     1141 tgtgtagcgt aacgctgcag tacaccagct gcatacagcg gccgtgcgcg tgatgttgtg
     1201 cagcctcctg gtcagcgtcg cgcggcggct gcggcgtccg cgagctcgtc gacggtaagc
     1261 cttctgtcga acgatggcga gtcgaccgcg agtctgcgtc gccgagcctc gacccggagc
     1321 cgcgcgatct cggcgcgaag ccgggcgagt gactcgcgat gctggcggcc gacgtcggcg
     1381 aaggtgcgct tgaacagctc gccgagctgc tcgtcgctcg gctcgaacga cggaatcccg
     1441 cgcgaggagc cccgtacacc cgccgacggc gagagcgcga acgccggcta accaacgccg
     1501 agcaggtaga ccgcgaacga ggcgagccag tgctcgcccg cgtagtcgcc ggtggcgacg
     1561 tgggccaggc cggcggcggc gtgggcgtcg gccgaagccg tcagctgcga gcgcagcgga
     1621 tcgccggccg gcagggcggc agcgatggcg cgcaggcacc acgcgcgcga caggttgagg
     1681 ccgtcgaggt gggcgagctt gccgtcgctg cgatcggtga cgatcgccgg ctgctggagc
     1741 gacggcggca ggccgcgcgg caggaacgcg tgcagccagg ccgggaactc ggccggcggc
     1801 agcacgcggc gcatcaggtc ggcctcgatc agcgacggcg acaggaactc gtcgccgctc
     1861 ggctcccacg ccgccggggc gtcgcgatcg gctgcgtagt aggcggccga gcgctcgacg
     1921 atcagcgcct cgagctcgcg gtggccgacc gcgcgcgcgt ggtcgcgcgc gagcgcgaag
     1981 ccgaacgcgg tgctggagtg gacgccggtg cggatggggt aggtctgcct ggggaagaac
     2041 gcgaggtagc gcgcgacgag caggtcggtc agcggctgca ggtgagccgc ccagcgccgg
     2101 ccgtcggcat cgtcccaggt gtggagctcg gcggcgagct ggagcagcca ggcccagccg
     2161 tacggccgct cgtagctggc ccggttgggg gcggcgaagt aggcg
//
LOCUS       DASVOR010000895         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67302,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000895 DASVOR010000000
VERSION     DASVOR010000895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67302"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..>1703)
                     /locus_tag="VF516_17455"
     CDS             complement(47..>1703)
                     /locus_tag="VF516_17455"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8109524.1"
                     /translation="VCGNCSVGCNVRVDVRVDKLLRQYSRTNDDIDDGFLCDRGRWDT
                     ETVNSPDRLRTPLIRRNGQLQPASWEEALTLIAGRLRDIKGQHGSQAIGGIGSTHTTN
                     EEAYLFQKLLRAGLGTNNIDHYHGAFPTTERNALPWVWTDSIAGLEKASHIVLIGADA
                     YHRQPIVDLRIRKAIRGGARVYVVTPEPNRLDRLAAGVIRYRAGQTTAVARALLNVVT
                     SEQLTRGAFAEQRAASIATRGASVAQDTPERAAEVAGVDATTLRELAREIAGAKGAVI
                     LYDEMATREPNAATLAADLLDLALLTDNYGRPGAGVGPLFEDNNSLGARDMGLLPDTL
                     PGYRPVNDLAGRSALSIAWNAPIPAEPGKTYEQMLDGGVKALYVLGANPAGHASVQQL
                     AKLEALDLLVVQDLFLTETAQRATVVLPALAYTEKDGSFTNTERCVQVVRQAMQPLPG
                     AKADWEILLGLARALGLGWTYLSPAEILSEIGRTTPLYAGVTRRALGDKGARWPLAAG
                     ERTAEGRATVTGSPYLTWQMIEQGVAPVSAQAGELVAPRGGGE"
BASE COUNT          254 a          568 c          594 g          287 t
ORIGIN      
        1 ttgaccagcg cggcgatgag aaagattgcg agatccgaca tagtctctat tctcctcccc
       61 cgcgcggggc aaccagctca ccggcctgcg cgctcaccgg cgcgacgccc tgctcgatca
      121 tctgccatgt cagataggga ctgccggtca cggtcgcgcg tccttcggcg gtgcgctcac
      181 cagcggccag cggccagcgg gcacctttgt cgcccaacgc gcggcgcgtc acaccggcat
      241 agagcggcgt cgtgcgcccg atctccgaga ggatttccgc cggtgagagg tacgtccagc
      301 cgaggccaag cgcccgcgct aggccgagga ggatctccca gtcggctttc gcgccgggca
      361 gcggctgcat cgcctgccgc accacctgga cacagcgctc ggtgttggtg aagctgccgt
      421 ccttttcggt gtaggccagc gcgggcagca ccaccgtcgc gcgctgcgcc gtctccgtca
      481 ggaagagatc ctgcaccacc agcagatcga gcgcctccag cttcgcaagc tgctgcacgg
      541 aggcatggcc ggcgggattc gcgccgagca cgtagagcgc tttgacgccg ccgtcgagca
      601 tttgctcgta ggtcttgcct ggctcggcgg ggattggggc attccaggcg atgctgagcg
      661 ccgagcgccc cgccagatcg ttcaccgggc gatatccggg cagcgtgtcg ggcagcaagc
      721 ccatgtcacg cgcgccgagc gagttgttgt cctcgaagag cggtccgacg cctgcacccg
      781 gtcgcccgta gttatccgtc agcagcgcga ggtcgaggag gtcggcggcg agcgtcgcgg
      841 cgtttggctc gcgtgtcgcc atctcgtcgt agagaatgac cgcgcccttc gcgccggcaa
      901 tctcgcgggc aagctcacgc agcgtggtgg catccacccc ggctacctct gcggcgcgct
      961 ccggcgtatc ctgcgcgacc gatgcgccgc gcgtggcgat gctggcggcg cgctgctcgg
     1021 cgaatgcgcc gcgcgtcaac tgctcactcg tcacgacgtt gagcaacgcg cgggcgacgg
     1081 ccgtggtctg gccggcgcgg tagcggatca cgcccgccgc gagtcggtcc agccggttcg
     1141 gctcaggcgt gacgacgtag acgcgcgcac caccgcggat cgccttgcgg atgcgcagat
     1201 cgacgatggg ctggcggtga taggcgtccg cgccgatcag gacgatgtgc gaggcttttt
     1261 cgagtcccgc aatcgaatcg gtccagaccc agggcagcgc gttgcgctcg gtcgtgggga
     1321 acgcgccgtg gtagtgatcg atattgttgg tgccgagacc ggcgcgcagc agcttctgga
     1381 agaggtaggc ttcctcgttg gtggtgtggg tcgagccgat gccgccgatg gcctgcgagc
     1441 cgtgctggcc cttgatgtcg cgcagccgcc cggcgattaa ggtcagcgcc tcctcccaac
     1501 tggcgggctg aagctggcca ttgcgccgga taagcggcgt gcgcaggcgg tcggggctat
     1561 tgacggtctc tgtatcccag cggccacggt cgcagaggaa gccatcgtcg atatcgtcgt
     1621 tggtgcgcga gtactggcgc agcagcttat cgacgcgcac atccacgcgc acgttgcagc
     1681 cgaccgagca gttgccgcaa acg
//
LOCUS       DASVOR010000896         2330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_476795,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000896 DASVOR010000000
VERSION     DASVOR010000896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476795"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..127
                     /locus_tag="VF516_17460"
     CDS             <1..127
                     /locus_tag="VF516_17460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8109525.1"
                     /translation="TCEEVAAAIVFLASPAAQFITGATLRLDGGQSLWGHSWEIP"
     gene            263..1024
                     /locus_tag="VF516_17465"
     CDS             263..1024
                     /locus_tag="VF516_17465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8109526.1"
                     /translation="MDLELTGKLCLVSGASRGIGRAIALALAREGARIAAVARNRDDL
                     DALLAELPGAGHTAIVADAASEEGASAAVDGCVKVLGGIDVVVAVVGKSFARSASAMD
                     DADLASSLDANLWSAARVAQRAVPHLIARGGGSITMLASIWGREAGGAPGYNTAKAAV
                     IALAKALARDYARHAIRVNSVAPGSILFPGGGWDRRRQADPDGIAAFVEREIPMGRFG
                     TPDEVAEVVAFLSSARARWITGACIPVDGGQGRSF"
     gene            1554..>2330
                     /locus_tag="VF516_17470"
     CDS             1554..>2330
                     /locus_tag="VF516_17470"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109527.1"
                     /translation="MTSSEPPAPTVRIEVTLGETVGRYQVRRPLGAGGMGQVYLARDV
                     VLGRSVALKIVGLDRSIEVSTEQFLHEARAIARLNHPNIVQLYDFGEYKGGFYLALEY
                     VEGDTLRERVRAGTMALDEALRHARAIADGLCHAHDHGVYHCDLKPSNVMVGKDGRVR
                     VVDFGIARTGDSRATASSGTPDWMAPEQWTGGRVTDRVDAWSLGIVTAQLLTGRHPLG
                     DAPEVRREAARSPTRIAAWTPERRDVPPAVIELVTRSLERE"
BASE COUNT          319 a          825 c          856 g          330 t
ORIGIN      
        1 cacctgcgag gaggtcgccg ccgcgatcgt cttcctcgcc tcccctgccg cccagttcat
       61 caccggcgcc acgctccgcc tcgacggcgg ccagtccctg tggggccaca gctgggagat
      121 cccgtagcgg tccgctgcca aggccgtgcg cgctgcgcga cgcgcttcgt acaaaaaacc
      181 gccgtatctg acgtcaggcg gaccttgcca aaactgtcgg tcccagaata cctcccgcgg
      241 cgccgtgggg gatactgggc tcgtggacct cgagctcacc ggcaagctgt gtctggtcag
      301 cggcgcgagc cgcggcatcg gccgcgcgat cgccctggcc ctggcccgcg agggcgcacg
      361 gatcgcggcg gtggcgcgca accgcgacga cctcgacgcg ctgctcgccg agctgcccgg
      421 cgcagggcac accgcgatcg tcgccgacgc cgccagcgag gaaggcgcca gcgcggcggt
      481 cgatggctgc gtcaaggtgc tcggcgggat cgacgtcgtg gtcgccgtgg tcggcaagtc
      541 gttcgcccgc agcgcctcgg cgatggacga cgccgacctc gcgagctcgc tcgacgccaa
      601 cctgtggagc gcggcgcggg tcgcccagcg cgccgtgccg cacctgatcg cgcgcggcgg
      661 cggctcgatc acgatgctcg cgtcgatctg gggccgcgag gccggcggcg cgccgggcta
      721 caacaccgcc aaggccgcgg tgatcgcgct cgccaaggcg ctggcccgcg actacgcgcg
      781 ccacgcgatc cgcgtcaact cggtcgcgcc gggctcgatc ctgttccccg gcggcggctg
      841 ggaccggcgg cggcaggccg atcccgacgg catcgccgcg ttcgtcgagc gcgagatccc
      901 gatgggccgg ttcggcacgc ccgacgaggt cgccgaggtc gtcgcgttcc tgtcgtcggc
      961 gcgcgcccgc tggatcaccg gcgcctgcat cccggtcgac ggcggccagg gccgcagctt
     1021 ctagatcacc cggcgaacgg cctcacgtgc tcggcatcgc gcggcatcgc gcggcatcgc
     1081 gcatcctggg gtggtttcgc acactcgcgc ggacgatggt cgttggtgtc ggaaccgcac
     1141 tgcgcgcgat ggtgagcgag ctcgatccgg cccgggctcc cggcgcgccc ggcatggccg
     1201 ggttcttgcc gggctgattt gttcgcgatc gcggtggagc gagatgactc gtatacgata
     1261 caggtgagtt cgtccgctcg agctgctcgc gcgcgccacg ggcgcagtgt gctgggtcgc
     1321 gagcgcggct acaccggcgt acctgaatga ctgcatcgaa acccagcacc gagcacgggc
     1381 gtcagaggct gtctggctta cgttgcttac agctgctcga taacgccgcg gggcggcttg
     1441 cgagatcgcc gatcagtccg tagcttctgc gggacagggg gatgggcgac gacggcgacg
     1501 acggtacggg tctcgaggac atggtggcgg aacagccgac gctctcgctt gcggtgacct
     1561 cctcggagcc accggcaccg acggttcgca tcgaggtcac gctcggtgag acggtcgggc
     1621 gctatcaggt ccggcgcccg ctcggcgcgg gcgggatggg ccaggtctac ctcgcgcgcg
     1681 atgtcgtgct cggccggtcg gtcgcactca agatcgtcgg gctggaccgc agcatcgagg
     1741 tctcgaccga gcagttcctc cacgaggcgc gggcgatcgc gcggctcaac caccccaaca
     1801 tcgtccagct ctacgacttc ggcgagtaca agggtggatt ctacctcgcg ctggagtacg
     1861 tcgagggaga cacgctgcgc gagcgggtgc gggccggcac gatggcgctc gacgaagcgc
     1921 tgcgccacgc ccgcgcgatc gccgacggcc tgtgccacgc ccacgaccac ggcgtgtacc
     1981 actgcgatct caagccgagc aacgtgatgg tcggcaagga tggccgggtc cgcgtggtcg
     2041 acttcgggat cgcgcgcacc ggcgacagca gggcgaccgc gtcgtccggc acgccggact
     2101 ggatggcgcc ggagcagtgg accggcggcc gggtgaccga tcgggtcgac gcctggtcgc
     2161 tcgggatcgt gaccgcgcag ctgttgaccg gacgccaccc gctgggcgac gccccggagg
     2221 tgcgccgcga ggccgcccgc agcccgacgc ggatcgccgc gtggacgccg gagcggcgcg
     2281 acgtgccgcc ggcggtgatc gagctcgtca cccggtcgct ggagcgcgag
//
LOCUS       DASVOR010000897         5236 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_242788,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000897 DASVOR010000000
VERSION     DASVOR010000897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5236
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242788"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF516_17475"
     CDS             <1..196
                     /locus_tag="VF516_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109528.1"
                     /translation="QMDACLLARDHGVPPTLVAEALGLTAEQVARVYRDIDAKRAAAR
                     YLHAPPLLVDPPGIPGGWGP"
     gene            755..1660
                     /locus_tag="VF516_17480"
     CDS             755..1660
                     /locus_tag="VF516_17480"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HEX8109529.1"
                     /translation="MNAVGPAPIRSFNRYELKYLIHASEIERIVADVLGQMSPDRYGD
                     ASGSYVISNLYYDSPELHMLRSKIVGNNYRRKVRVRTYGDGSGQPPRTAMVEIKQRLS
                     RTTQKRRVVVPLDEALRICEARSTWEWDDAADRAVAEEIRCLVLALALRPACTISYRR
                     RAFVGSRFEPGLRLTIDTDLWGAPPRLDFYTQRPSAFLMPRDWSILEIKVDDRVPSWI
                     NELVTRHDCQLRRVSKYCLGMAKLHNIMLPMAAWPSSSDPRPGELVRAEPATSIVPLP
                     VRHMPSSPLDTNRHEEQLSAATRTP"
     gene            1657..2367
                     /locus_tag="VF516_17485"
     CDS             1657..2367
                     /locus_tag="VF516_17485"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HEX8109530.1"
                     /translation="MNPSDHKALSDAFAGVLDLSAAIPPEQIAVSLALSLVLSLAIAT
                     TYRITHRGVSYSQTYAHTLVIMSLVITVIMLIVGSSIARAFSLVGAMSIIRFRNAVKE
                     TKDIGFIFLVMAIGMACGARFYTLAAIAAFLLCTVILLLFRANMFGKAQSSRILCVRM
                     PADRDHDGTLAPVFSRYLDDSSLISMESVGGGSLQELVYSVVLKRNAEPRRLIDSLRH
                     VNENQKVSLVLGQQEVDL"
     gene            2364..3461
                     /locus_tag="VF516_17490"
     CDS             2364..3461
                     /locus_tag="VF516_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109531.1"
                     /translation="MRGPLRKKAWVLVPSLLALIAMVPTYSWTARYRMPGEARVRQLT
                     FDTKGPDLVINEFAVASPDGKYFLIQHSEDPKLVTQPDGTKTMDFKETSNWDIYRYNV
                     DGTGRLQLTSDPTSEDQPTWSPDGKTIVYRKLNGKQFDLYLMDADGSNKRELLIDPAH
                     DEKTPAFSRDGKKVVFFSDRDGIKWNLYTIDIATRKVERLTHDQVEDKHPQFTPDGHV
                     VFHSSRDSTHVTLEGGEEFDIMNLWSLDPATGTLAELTSSGSFSSAYGMRDNRHAWIS
                     PDGKYVAFHSNVIDADRKHPGIYRKRHRELCIATIDGSRRVYLTMGDHRNFKHPSWAA
                     DGRGLFFVFKEKGHAWNAGFMDVSDALERLR"
     gene            3497..4123
                     /locus_tag="VF516_17495"
     CDS             3497..4123
                     /locus_tag="VF516_17495"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX8109532.1"
                     /translation="MLRKLLPRETSFFDYFEQHAELTLDGATEFLSLVSSGVNIEVKA
                     RRIKEIEHETDVITHRCVEALHKTVITPMDSAQIHRLITRMDDVMDAIEAAAERIALY
                     KLRQMTPEVQAMADVLKSATQVLLDACRLLRNVRDPEAILKKCIDVNRLENEADAILR
                     CAVARLFDQEDDPVMIIKWKEIYESLETAADRCEDVANIIENVILDLT"
     gene            4132..5142
                     /locus_tag="VF516_17500"
     CDS             4132..5142
                     /locus_tag="VF516_17500"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX8109533.1"
                     /translation="MAITTVVIVTVLVALAFDVINGFHDAANSIATIVATRLMTPRKA
                     VAWAAFFNFVALFIFHQGVAKTVSSLVKVDARDPAFVYVVLAGLIGAVLWNLLTWFLA
                     LPSSSSHAIMGGFSGAGLAYGGVGVLDLPKLRITAEFIVIAPVIGLALGSVLLVIVSW
                     LIRRLRPPQVQRMFHGIRMFSAAAYSIGHGGNDAQKTMGIILAVLIAGGHVSSGATVG
                     PTWVAVACYSAMAIGTAMGGWRIIKTLGSRLTKLSSHGAFCAELSGAVTLFAATALQV
                     PVSTTHTITGAIVGVGSTNKLRGIRWGLASNIVWAWVLTIPASAGIGIACVKLFEKAG
                     VI"
BASE COUNT          895 a         1746 c         1717 g          878 t
ORIGIN      
        1 ccagatggac gcctgcctgc tcgcccgcga tcacggcgtc ccgccgacgc tggtcgccga
       61 ggcgctcggc ctgaccgccg agcaggtcgc gcgggtctat cgcgacatcg acgccaagcg
      121 cgcggccgcc cgctacctgc acgcgccgcc gctgctggtc gatccgcccg ggatccccgg
      181 cgggtgggga ccgtgaccta ggcctcctcg tcgaggtccg tcaggtaccc ggcggtgcgg
      241 gcgggcagcg ccgcgatcgc cggcccctgc aacacactgt cccctcacag cgacggatcg
      301 aggaaccgcc tggttcctcg caactcagtg cccctccaca gggacggatc gaggaagcgc
      361 ctggttcctc gcaacagggc gtccatggac ggcgaggcag cgcggacgga ctggtgcgca
      421 gagtgctgtg ctgccggaac gcggagtgac tctcgacgct gcgctcgctg aaatcgaatt
      481 gcaaaatcga attgcaattc acgtacgacg ccggctggaa acacatccga aacaggcatg
      541 gaacggccct gacgagatgt gaatcccgcc gtccgaagct tgcacatcgc gcggggttga
      601 tcccgggcag cttacggttc gcatggtgga tgttcgatct tcggcggaca gccccggcct
      661 cgattgcaat gtcgattgca gttcttcggc atcgtgagtt ttgcgtgaag atcccgttgc
      721 ataatcttcg caaaaatgtc acttgactgg acccatgaac gcggtgggtc ccgctccgat
      781 tcggagcttc aatcggtacg agctcaagta tctgattcac gcatcggaga tcgagagaat
      841 cgtcgccgac gtcctcggcc agatgtctcc cgatcgctat ggcgatgcca gcggttcgta
      901 cgtgatttcg aatctttatt acgacagtcc cgagctgcac atgctgcgct cgaagatcgt
      961 cggcaacaac taccgccgca aggtgcgcgt ccggacctac ggcgacggga gcggccagcc
     1021 gccgcggacc gcgatggtcg agatcaagca gcggctcagc cgcaccacgc agaagcggcg
     1081 cgtggtggtc ccgctcgacg aggcgctgcg gatctgcgag gcgcggagca cgtgggagtg
     1141 ggacgacgcc gccgatcgcg ctgtggccga agagatccgg tgcctggtcc tggcgctcgc
     1201 gctgcggccg gcgtgcacga tctcgtatcg ccgccgcgcg ttcgtcggca gtcggttcga
     1261 gcccgggctc cggctgacca tcgacaccga tctatggggc gccccaccgc ggctggactt
     1321 ctatacccag cgtccgagcg cgttcctcat gccgcgcgac tggtcgatcc tggagatcaa
     1381 ggtcgacgac cgggtcccga gctggatcaa cgagctggtg acccggcacg actgccagct
     1441 tcgccgcgtc agcaagtact gcctcggaat ggccaagctt cacaacatca tgctgccgat
     1501 ggcggcgtgg ccgtcgagca gcgatcccag gccgggcgag ctcgttcgcg ccgagccggc
     1561 cacgtccatc gttccattgc ctgtccggca catgccgtct tcccccctgg acaccaaccg
     1621 ccatgaagag cagctgagcg ctgccacgag gaccccatga atcccagtga tcacaaggct
     1681 ctgtccgacg cgttcgcggg cgtgctcgat ctgagcgccg cgattccccc cgagcagatc
     1741 gcggtcagcc tggccctgag cctggtgctc agcctggcaa tcgcgacgac ctatcgcatc
     1801 acccaccgcg gcgtctcgta ctcgcagacc tacgcccaca ccctggtcat catgtccctg
     1861 gtgatcacgg tgatcatgct gatcgtcgga tcgagcatcg cccgggcgtt ctcgctggtc
     1921 ggcgcgatgt cgatcatccg gttccgcaat gcagtgaagg agaccaagga catcgggttc
     1981 atcttcctgg tcatggcgat cggcatggcg tgcggcgcgc ggttctacac gctggcggcg
     2041 atcgcggcgt tcctgctgtg caccgtcatc ctgctcctgt tccgcgccaa catgttcggc
     2101 aaggcgcaga gctcgcggat cctgtgcgtc cggatgccgg ccgaccgcga tcacgatggc
     2161 acgctggcgc cggtgttctc gcgctacctc gatgacagca gcctgatctc gatggagtcg
     2221 gtgggcggcg gcagcctgca ggagctggtg tacagcgtgg tgctcaagcg caacgccgag
     2281 ccgcgccggc tgatcgattc gctgcgccac gtcaacgaga accagaaggt cagcctcgtg
     2341 ctggggcagc aagaagtcga cctgtgaggg gaccgctgcg caagaaggcg tgggtcctgg
     2401 tgccgtcgct gctcgcgttg atcgcgatgg tgccgaccta ctcctggacg gcgcgctacc
     2461 ggatgcccgg cgaggcgcgc gtccgccagc tcacgttcga caccaagggg ccggacctgg
     2521 tgatcaacga gtttgcggtc gcctcacccg acggcaagta cttcctgatc cagcactccg
     2581 aggaccccaa gctggtgacc cagcccgacg gcaccaagac gatggacttc aaggagacct
     2641 cgaactggga catctaccga tacaacgtcg acggtacggg gcgtctgcag ctcaccagcg
     2701 atccgacgtc ggaggaccag ccgacctggt cgccggacgg caagaccatc gtctaccgca
     2761 agctcaacgg caagcagttc gacctctacc tgatggacgc cgacggcagc aacaagcgcg
     2821 agctgctgat cgaccccgcg cacgacgaga agaccccggc gttctcgcgc gacggcaaga
     2881 aggtcgtgtt cttctccgac cgcgatggca tcaagtggaa cctctacacg atcgacatcg
     2941 cgacccgcaa ggtcgagcgc ctgacccacg accaggtcga ggacaagcac ccgcagttca
     3001 cgccggacgg tcacgtcgtg ttccactcca gccgcgattc gacccacgtg acgctggagg
     3061 gcggcgagga gttcgacatc atgaacctgt ggtcgctcga tccggccacc gggacgctcg
     3121 ccgagctgac ctcgtcgggg tcgttcagct cggcctacgg gatgcgcgac aaccggcacg
     3181 cctggatctc cccggacggc aagtacgtcg cgttccacag caacgtcatc gatgccgatc
     3241 gcaagcaccc cgggatctat cgcaagcgcc atcgcgagct gtgcatcgcg acgatcgatg
     3301 gctcgcgccg ggtgtacctc acgatgggcg atcaccggaa cttcaagcac ccgagctggg
     3361 cggccgatgg caggggcctg ttcttcgtgt tcaaggagaa gggccacgcc tggaacgccg
     3421 ggttcatgga cgtcagcgac gcgctcgaac ggctgcgctg aatcgcaacc cacgaccaac
     3481 gcaaggagct gcacgcatgc tgcgcaagct gctgcccagg gaaacctcgt tcttcgacta
     3541 cttcgagcag cacgccgagc tcacgctcga cggcgcgacc gagttcctgt cgctggtcag
     3601 ctcgggggtg aacatcgagg tcaaggcgcg ccggatcaag gagatcgagc acgagaccga
     3661 cgtgatcacg caccgctgcg tcgaggcgct gcacaagacc gtgatcacgc cgatggattc
     3721 ggcgcagatc caccggctga tcacgcggat ggacgacgtg atggacgcga tcgaggccgc
     3781 cgccgagcgc atcgcgctgt acaagctgcg ccagatgacg cccgaggtcc aggcgatggc
     3841 agacgtgctg aagtccgcga cccaggtctt gctggatgcg tgccggctgc tgcgcaacgt
     3901 ccgcgacccc gaggcgatcc tgaagaagtg catcgacgtc aaccggctgg agaacgaggc
     3961 cgacgcgatc ctgcgctgcg cggtcgcgcg gctgttcgat caggaggacg acccggtgat
     4021 gatcatcaag tggaaggaga tctacgagag cctggagacc gcggcggatc gctgcgagga
     4081 cgtggccaac atcatcgaga acgtcatcct cgatctgacc tgaggtgccc gatggctatc
     4141 acgactgtcg tcatcgtcac ggtgctggtg gcgctggcgt tcgatgtcat caatggcttt
     4201 cacgacgcgg ccaacagcat cgccacgatc gtggcgaccc ggctgatgac gccgcgcaag
     4261 gcggtggcct gggccgcgtt cttcaacttc gtcgcgctgt tcatcttcca ccagggcgtg
     4321 gcaaagaccg tgtccagcct ggtcaaggtc gacgcccgcg atccggcgtt cgtgtacgtc
     4381 gtgctcgccg gcctgatcgg cgcggtgctg tggaacctct tgacctggtt cctcgcgctg
     4441 ccgtcgagct cgtcgcacgc gatcatgggc gggttctccg gcgccgggct ggcctatggc
     4501 ggcgtcggcg tcctcgacct gcccaagctc cggatcaccg cggagttcat cgtgatcgcg
     4561 ccggtgatcg ggctcgcgct cggcagcgtg ctgctcgtca tcgtctcgtg gctgatccgc
     4621 cggctgcgcc cgccgcaggt ccagcggatg ttccacggca tccgcatgtt ctctgccgcc
     4681 gcgtactcga tcggccacgg cggcaacgac gcgcagaaga cgatggggat catcctggcc
     4741 gtgctgatcg ccggcggcca cgtgtcgtcc ggcgcgacgg tcggtccgac ctgggtcgcg
     4801 gtcgcgtgct actcggccat ggcgatcggc accgcgatgg gcggctggcg gatcatcaag
     4861 acgctcggct cgcggttgac caagctgagc tcgcacggtg cgttctgcgc cgagctgtcg
     4921 ggcgcggtga cgctgttcgc cgccaccgcg ctccaggtcc cggtgtcgac cacgcacacc
     4981 atcaccggcg cgatcgtggg cgtcggctcg accaacaagc tgcgcgggat ccggtggggc
     5041 ctggccagca acatcgtgtg ggcctgggtg ctcacgatcc ccgcctcggc gggcatcggg
     5101 atcgcctgcg tcaagctgtt cgagaaggcc ggcgtgatct gacaggtatt ctctaggtat
     5161 cctcatcgag gtcggtcagg taccaggcgg ggcgggcggg cagcgccgcg cgggccgagc
     5221 cgcggccggg cgcgac
//
LOCUS       DASVOR010000898         5082 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_213673,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000898 DASVOR010000000
VERSION     DASVOR010000898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5082
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213673"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VF516_17505"
     CDS             complement(<1..544)
                     /locus_tag="VF516_17505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109534.1"
                     /translation="MRIAWIVISLVCIAVPARGIAGPKPKIAVAPLDGDPGNKVAAAV
                     VEALAGKDYAVIGPKETGREIARLSLPGELDAKATRKLTTKLGVVAVLDGKVSKAGGK
                     RFLHLEVHRRGKPDAGFTVELKSTTSKAFRRDLHDEVDRKLEGASDDPGDDEARPAPF
                     ADDKRKPADAGDERPARTASE"
     gene            complement(612..1976)
                     /locus_tag="VF516_17510"
     CDS             complement(612..1976)
                     /locus_tag="VF516_17510"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8109535.1"
                     /translation="MPLQRRLHRIDALAIALGTVIGIGVFRNTGMVLRGAGGFGGATL
                     LWLVSGVLVLGGAVLYADLSGRVPEAGGPYAYVRVAFGGKAAFAYGWMNAGVAIPARQ
                     ASTAAITGELLSKWIPVSASTLALALYLVLAVIHLFGVRAGAIAQRIFTAGKLLTVML
                     VVVLAVTLGAMGTGMTGAPAGPAGEPAVSFGLALSAVWYTYLGWQDVVLLAEELHEPR
                     RDLPLVMIGTVLLVMVVFTALHVAVFWGLGGGAEAYDMEYPALHVASRMLDGFGDNLL
                     RVLMVSSLIGVTAGGLLVRPRIGMALARDGLGPAPLTRVSRVGTPYVALGLQVAITSG
                     MILTGSYVKLLELISFAMGVLGLFEIASYFVVRKKRPALPTSRLHPWAPMAFLIMSGA
                     LCVLSGWDHPMGVVTAFAILIAIALIYAGTRPQLIPVEVAPATLDLPEARAREGTPRP
                     DAET"
     gene            complement(2084..2656)
                     /locus_tag="VF516_17515"
     CDS             complement(2084..2656)
                     /locus_tag="VF516_17515"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX8109536.1"
                     /translation="MDDTALRVALERLIADSERARLGVAELQQQFETLIEIMVAAGTL
                     KPGHAELIAKLRRRVEIARKAPVELSSIEDKYVATGEPIDCESRLPLCQARCCSFAVA
                     LSRQDLAEGQLVWEIDQPYRLARGPDGYCGHLGRDDARCQRYEHRPAVCRVYSCRTDQ
                     RVWLDFEARIPAPMPVSLIPLHRLTARRDG"
     gene            2705..3355
                     /locus_tag="VF516_17520"
     CDS             2705..3355
                     /locus_tag="VF516_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109537.1"
                     /translation="MPGLLDLIDDDPTGIDAIAAYLDGLDPQQRWDEVSRLDRDHQRT
                     LYEKAAHAAPIDIAHFVGTAGPRQEVIHDGLNTLPVAARYRRFQKRFCRPEPAGGNGN
                     GHGPIDTRLFGYNEGPSRRWIGPGFFVAVPTAGRPQWTARGGVVVDYYQVPDGEVSEG
                     WPRIVPNNWRLQRFVYHQTRDFMRRVSRDVSIGAAYKRERPLDHYFVLCRRPPEAS"
     gene            3497..4873
                     /locus_tag="VF516_17525"
     CDS             3497..4873
                     /locus_tag="VF516_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109538.1"
                     /translation="MRAMLVGCAIIAGCGGGGGGGTPPTGDGGGSDGPNQNATKITFT
                     LTSAPSDVEAFGFVAAYQDGDGAWKPAPARSGDTYAFDVSSATWGFAYTCQVNVRIAG
                     VVTDLRDVVEYHFAVAERTSLTDDILTRCTDRIATVELSGTISPLPSAGTTYRVAYND
                     TAVFIDRTTGKYDMLVAPGTHDLLVLHGSEHNTPGDFLIDAAVVQRNLAVTAAMTRDV
                     AANNATATKSLSVSVSVPGQTPMSSETTTNLFTANGTAVSMAHVTKPPQTQLASTALA
                     NSQLAAGDVYDQRVTVEFVKGQAVTTTMATTATTTTPANVSITTAPTPLGATPMTTSA
                     ATMPYPRFRSTWPTYAGAVGYTRTLAQTPPAAGCGGSPACTITWTAWVAPGAVGAMPS
                     YTMPDLSQLPGWSTALGFVAGTMATGDVTAMTSSVGAMDFPPQPPVAGTKRVFAHSTF
                     TVTPTATP"
BASE COUNT          758 a         1917 c         1725 g          682 t
ORIGIN      
        1 cctcgctcgc ggtgcgggcg ggccgctcgt cgcctgcgtc ggccggcttg cgcttgtcgt
       61 cggcgaacgg cgccggcctg gcctcgtcgt cgcccgggtc gtcgctcgcg ccctcgagct
      121 tccggtcgac ctcgtcgtgc aggtcgcgcc ggaacgcctt cgacgtggtc gacttgagct
      181 cgacggtgaa gccggcgtcg ggcttgccgc ggcgatggac ctcgaggtgc aggaagcgct
      241 tgccgccggc cttgctgacc ttgccgtcga gcaccgcgac gacgccgagc ttggtcgtca
      301 gcttgcgggt cgccttggcg tcgagctcgc cgggcaggct gagccgcgcg atctcgcggc
      361 cggtctcctt cgggccgatc accgcgtagt ccttgccggc cagcgcctcg acgaccgcgg
      421 cggcgacctt gttcccgggg tcgccgtcga gcggggccac cgcgatcttc ggcttgggcc
      481 cggcgatccc gcgggccggt accgcgatgc acaccagcga gatcacgatc catgcgatgc
      541 gcaccggcca agcatagtcc gtcctcggct gcgcgggatc acccacacgt gatccagctc
      601 gcaatccggc gttacgtttc ggcgtccgga cgcggcgtgc cctcgcgcgc gcgcgcctcg
      661 ggcaggtcca gcgtcgccgg cgcgacctcg acggggatca gctgcgggcg ggtccccgcg
      721 tagatgagcg cgatcgcgat caggatcgcg aacgccgtga cgacgcccat cgggtgatcc
      781 cagcccgaca gcacgcacag cgcgccgctc atgatcagga acgccatcgg cgcccacggg
      841 tgcagccggc tggtcggcag cgcgggccgc ttcttgcgca ccacgaagta gctcgcgatc
      901 tcgaacaggc cgagcacccc catcgcgaac gagatcagct cgagcagctt gacgtagctg
      961 ccggtcagga tcatcccgga cgtgatcgcg acctgcagcc cgagcgcgac gtacggcgtg
     1021 ccgacccggc tgacccgggt cagcggcgcc ggccccagcc catcgcgggc cagcgccatc
     1081 ccgatccgcg gccgcaccag cagcccgccc gcggtcacgc cgatcagcga cgacaccatc
     1141 agcacgcgca gcaggttgtc gccgaaaccg tccagcatgc gcgacgcgac atgcagcgcg
     1201 gggtactcca tgtcgtaggc ctcggccccg ccgcccagcc cccagaacac cgcgacgtgc
     1261 agggcggtga acaccaccat cacgagcagc acggtgccga tcatcaccag cggcaggtcg
     1321 cgccgcggct cgtgcagctc ctcggcgagc agcaccacgt cctgccagcc gaggtaggtg
     1381 taccagaccg cgctcagcgc gaggccgaac gacaccgcag gctccccggc aggcccggcc
     1441 ggcgcgccgg tcatgccggt gcccatcgcg cccagcgtca ccgcgaggac cacgaccagc
     1501 atcaccgtca gcagcttgcc cgcggtgaag atccgctgtg cgatcgcgcc ggcgcggacg
     1561 ccgaacaggt ggatcacggc gagcacgagg tacagcgcca gcgccagcgt gctcgcgctc
     1621 accgggatcc acttcgacag cagctcgccg gtgatcgccg cggtcgacgc ctggcgcgcc
     1681 gggatcgcga ccccggcgtt catccagccg taggcaaacg ccgccttgcc accgaacgcc
     1741 acccgcacgt acgcgtaggg cccaccggcc tccggcacac gcccgctgag atcggcgtac
     1801 agcaccgcac cgccgagcac cagcacgccg gacaccagcc acagcagcgt cgcgccgccg
     1861 aagcccccgg cgccgcgcag caccatcccg gtgttgcgga acaccccgat gccgatcacc
     1921 gtgccgagtg cgatggcgag cgcgtcgatc cggtgaaggc ggcgctggag tggcaccacg
     1981 accgaaggtc cgccacgccg cggtggctgt caatcgggac gagcggctcg accgcgccgg
     2041 cggcccgacc gcgcggacgg cccgcggcgc gttggtgcgg gagctaaccg tcgcggcgcg
     2101 cggtgagccg gtgtagcggg atcagcgaga ccggcatcgg cgccgggatc cgggcttcga
     2161 agtcgagcca gacccgctgg tcggtccggc acgagtagac gcggcagacc gcggggcggt
     2221 gctcgtagcg ctggcagcgc gcgtcgtcgc ggccgaggtg gccgcagtag ccgtcggggc
     2281 cgcgcgccag ccggtacggc tggtcgatct cccataccag ctggccctcg gcgaggtcct
     2341 ggcgcgacag cgcgaccgcg aagctgcagc accgggcctg gcacagcggc agccggctct
     2401 cgcagtcgat cggctcgccg gtcgccacat acttgtcctc gatgctcgac agctcgaccg
     2461 gggccttgcg cgcgatctcg acccgccgcc gcagcttggc gatcagctcg gcgtgcccgg
     2521 gcttcagggt cccggctgcc accatgatct cgatcagcgt ctcgaactgc tgctggagct
     2581 cggcgacccc gagccgggcc cgctcgctgt ccgcgatcag ccgctccagc gccacccgca
     2641 aggcggtgtc gtccatcgcg cgaccttacc gcgcggcccg cgggatgcgc taggctcgcc
     2701 gccgatgccc ggcctgctcg acctgatcga cgacgacccc accggaatcg acgccatcgc
     2761 ggcctacctc gacggcctcg acccccagca gcgctgggac gaggtcagcc ggctcgatcg
     2821 cgaccaccag cgcacgctgt acgagaaggc ggcccacgcc gcgccgatcg acatcgcgca
     2881 cttcgtcggc accgccggcc cgcgccagga ggtcatccac gacggcctca acacgctgcc
     2941 ggtcgccgcc aggtacaggc ggttccagaa gcggttctgc cggcccgagc cggccggcgg
     3001 caacggcaac ggccatggcc cgatcgacac ccggctgttc ggctacaacg agggcccgtc
     3061 gcgccggtgg atcggcccgg gcttcttcgt cgcggtgccg accgccggcc gcccgcagtg
     3121 gaccgcgcgc ggcggggtcg tcgtcgacta ttaccaggtc cccgacggcg aggtcagcga
     3181 gggctggccg cgcatcgtgc ccaacaactg gcgcctgcag cgcttcgtct atcaccagac
     3241 ccgcgacttc atgcgccgcg tgtcgcgcga cgtgtcgatc ggcgccgcct acaagcgcga
     3301 gcggccgctc gaccattact tcgtgctgtg ccgccggccg cccgaggcca gctgacccgg
     3361 ttgtgatgac cattgcgcga gattgctcat gtgttttcgt taggaatact gactgccgga
     3421 tcgcgacccg ccgctgtgac gggcgtcgcg aaactcgatg cgaggaccgg gggcgcttca
     3481 tgataccgtc gcggccatgc gagccatgct cgtcgggtgt gcgatcatcg caggatgcgg
     3541 tggcggcggc ggtggtggca cgccacccac cggagacggc ggtgggagcg atggcccgaa
     3601 ccagaacgcg acgaagatca cattcacgct caccagcgcg ccgagcgacg tcgaggcgtt
     3661 cgggttcgtc gcggcctatc aggacggcga cggcgcatgg aagccagcgc cggctcgcag
     3721 cggcgacacg tatgcgttcg acgtgagctc ggcgacctgg gggttcgcgt acacctgcca
     3781 ggtcaatgtc cggatcgccg gcgtcgtgac cgatctccgc gacgtcgtcg agtatcactt
     3841 cgcggtcgcg gagcgcacct cgctgaccga tgacatcctg acgcggtgca ccgatcgaat
     3901 cgccaccgtc gagctgtcgg gcaccatctc tcccctcccg tcggccggga ccacctatcg
     3961 cgtcgcctac aacgacaccg cggtcttcat cgacaggacc actggcaagt acgacatgct
     4021 cgtcgcgccg ggcacgcatg acctgctggt cctccacggc agcgagcaca acacgcccgg
     4081 cgacttcctg atcgacgcag cggtcgtgca gcgcaatctg gcggtcaccg cggcgatgac
     4141 gcgggacgtg gccgcgaaca acgccacggc gaccaagtcg ctgtcggtct cggtgtctgt
     4201 gcccggccag actccgatga gcagcgagac cacgaccaac ctgttcaccg ccaacggcac
     4261 cgcggtgtcg atggcccacg tcaccaagcc gccgcagacg cagctcgctt cgaccgcgct
     4321 ggcgaactcg caactggcgg cgggcgacgt ctacgaccag cgggtcacgg tcgagttcgt
     4381 caagggccag gccgtgacca cgacgatggc gacgacggcg acgacgacga cgccggccaa
     4441 cgtgtcgatc accaccgccc cgacgccgct cggcgccacc cccatgacga cgagcgcggc
     4501 gacgatgccg tatccgaggt tcaggtcgac ctggcccacc tacgccggcg cggtcgggta
     4561 cacgaggacg ctggcgcaga cgcctccggc ggccggatgc ggcggctccc ccgcgtgcac
     4621 catcacgtgg accgcatggg tcgcgcccgg tgcggtcgga gcgatgccct cgtacacgat
     4681 gccggatctg tcccagctgc cgggctggag cacggcgctc ggcttcgtcg cgggcacgat
     4741 ggcgaccggc gacgtcaccg cgatgacgtc gagcgtgggc gcgatggact tcccgcccca
     4801 accgccggtc gccgggacca agcgcgtgtt cgcgcactcg accttcaccg tcacgcccac
     4861 cgccacgcct tgaccggcgt ggccgatcac cgatccgagc tccgatccga tcaccgatcc
     4921 tccgggtcgg aagccccgag cgcgcgctta cttccgcgtg tctcccgcgg cctctgtgcg
     4981 gcggcgctgc tcgcggcgct gctcgcggcg ctggccgcgc tcgaggagcg acagctgatc
     5041 ccgtcggcgc tcggcgccgc gcaccgcgcg gtctacgcga tc
//
LOCUS       DASVOR010000899         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417586,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000899 DASVOR010000000
VERSION     DASVOR010000899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417586"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /gene="hypB"
                     /locus_tag="VF516_17530"
     CDS             <1..486
                     /gene="hypB"
                     /locus_tag="VF516_17530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX8109539.1"
                     /translation="HDARRLAAAGITAATITTGSACHLDARLVHDALHDLPWRSFDLF
                     AIENVGNLVCPAIYDLGQAANVVALAVTEGEDKPLKYPVMFRKADLVLLTKIDLVPHL
                     DVDLDAIRDALSRVMPEPRMIAVSARTGEGIAEWLTWLEARRPQAAGAAAPHAHAHGS
                     P"
     gene            complement(493..639)
                     /locus_tag="VF516_17535"
     CDS             complement(493..639)
                     /locus_tag="VF516_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109540.1"
                     /translation="MSPTGGSGTRHQRLLAALLDPSTVVQRDAAELYPREDVVPRIVS
                     WWEL"
     gene            1073..>1999
                     /locus_tag="VF516_17540"
     CDS             1073..>1999
                     /locus_tag="VF516_17540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109541.1"
                     /translation="MLRERIDEGGFGAVYRCEQPLLGREAVVKVLHQRLRRNDTVLQR
                     FMREAQLASRLDHPYAAHVYAFGIEHDDGLFWISMELVHGTALNRWLRERGPLSLGQL
                     VPFFECVAEVVQTAHDRGIVHRDLKPSNVMVIERAGKLLPKLLDFGVAKLVDEGALPR
                     PPRLHAAGTQPLAVPGDRQFDTAEALTLTASTTPPQRHDLQHRLTHADSTIGSPPYMS
                     PEQWTNAVTVGPTSDLYALAVIAYEALTGRRPFTATTITEYADLHCNAAVPPLGDGLP
                     PALDRLFARALAKRPEQRWQSALELAAELRSAS"
BASE COUNT          306 a          741 c          671 g          281 t
ORIGIN      
        1 cacgacgccc gccggctcgc cgccgccggg atcacggccg ccacgatcac caccggctcg
       61 gcgtgccacc tcgacgcgcg gctggtccac gacgcgctcc acgacctgcc gtggcgatcg
      121 ttcgacctgt tcgcgatcga gaacgtcggc aacctggtgt gcccggcgat ctacgatctc
      181 ggccaggccg ccaacgtggt cgcgctggcg gtcaccgagg gcgaggacaa gccgctcaag
      241 tacccggtga tgttccgcaa ggccgacctc gtgctgctca ccaagatcga cctggtgccg
      301 cacctcgacg tcgacctgga cgcgatccgc gacgcgctgt cccgcgtgat gcccgagccg
      361 cggatgatcg cggtgtcggc ccgcaccggc gaggggatcg ccgagtggct cacctggctg
      421 gaggcgcggc gaccgcaggc tgccggcgcc gcggcaccgc acgcccacgc gcacggctcg
      481 ccctgacccg gttcacagct cccaccagct gacgatccgc ggaacaacgt cctcgcgcgg
      541 gtagagctcc gccgcgtcgc gctgcaccac ggtgctcgga tcgagcagcg ccgccagcag
      601 ccgctggtgg cgcgttccgg agccgccggt cggcgacatg atcgagaagc tccggtagat
      661 cctcggatca tttccctctg cccctgccat atccgaaagg cgacgcaccc ccacgcttcc
      721 ctacggaaga tctgtcggac cgcgtcgtgc gcccacgcgc ggcgggagcc ggcgggctcg
      781 gaaagctgag gggcccgtcg gatccgctat ccctgagcgt aaaggcgacg cacgccgggt
      841 ctaccttaca aggttcttca tgcgaatatc cctcgacgac gcccggcgaa agaattgagg
      901 gatccatcac accaaaaaga gcctccgatg ctggtgtatg ggcaccggca cacaccccga
      961 aaaccgtcgg tgcggcgcct gttctgttga tccgcgcccg ccgagcagtg ataccgtaac
     1021 ggcacgggtg ggggagcgcg aggatctttc aggtcgaacg ctcggagagt tcgtgctccg
     1081 cgagcgcatc gatgaaggcg gcttcggcgc ggtgtaccgc tgcgagcagc cgctgctcgg
     1141 ccgcgaggcg gtggtgaagg tcctgcatca gcggctgcgg cgaaatgaca cggtgctgca
     1201 gcgcttcatg cgcgaggccc agctggcgtc gcggctcgac cacccctacg ccgcgcacgt
     1261 ctacgcgttc gggatcgagc acgacgacgg cctgttctgg atctcgatgg agctggtcca
     1321 cggcaccgcg ctcaaccgct ggctgcgcga gcggggcccg ctgagcctcg gccagctggt
     1381 gccgttcttc gagtgcgtcg ccgaggtcgt ccagaccgcg cacgatcgcg ggatcgtgca
     1441 ccgcgacctc aagccgtcga acgtgatggt gatcgagcgc gccggcaagc tgctccccaa
     1501 gctgctcgac ttcggcgtcg ccaagctggt cgacgagggc gcgctgcccc ggccgccgcg
     1561 gctgcatgcc gccggcaccc agccgctcgc ggtgccgggc gatcgccagt tcgacaccgc
     1621 cgaggcactg acgctgaccg cgagcaccac gccgccgcag cgccacgacc tgcagcaccg
     1681 cctgacccac gccgactcga cgatcggctc gccgccgtac atgtcgcccg agcagtggac
     1741 caacgcggtg acggtcggcc cgacgtcgga cctctacgcg ctcgccgtga ttgcctacga
     1801 ggcgctgacc ggccggcgcc cgttcaccgc caccacgatc accgagtacg ccgacctgca
     1861 ctgcaacgcc gcggtgccgc cgctcggcga cgggttgccg ccggcgctcg accggctgtt
     1921 cgcgcgcgcg ctggccaagc gaccggagca gcgctggcag agcgcgctcg agctcgccgc
     1981 ggagctgcgc tcggcgtcg
//
LOCUS       DASVOR010000900         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_505700,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000900 DASVOR010000000
VERSION     DASVOR010000900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505700"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..336
                     /locus_tag="VF516_17545"
     CDS             <1..336
                     /locus_tag="VF516_17545"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8109542.1"
                     /translation="CFDDESIAICEDRRAQLTRRRGLVVQGLKAIGLDVPVVPDGAFY
                     VYINIRSTGLDAMAFCQRVLHEAHVALTPGYDFGHALASQHVRLSYAAAEADLVEALQ
                     RLGAVMARL"
     gene            complement(482..907)
                     /locus_tag="VF516_17550"
     CDS             complement(482..907)
                     /locus_tag="VF516_17550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8109543.1"
                     /translation="MILPDVNLVVYAYNSDAPHHAVARAWWEGLLNGTEPIAIPWVVV
                     LGFLRLMTHRFVLPHPMSPTVAFAQIRAWFAQPNVEHLEPGPQHLDLLDRLLSAVGTA
                     GNLTTDAHLAALAIEHQCELHSNDNDFARFPGLRWRNPL"
     gene            complement(904..1164)
                     /locus_tag="VF516_17555"
     CDS             complement(904..1164)
                     /locus_tag="VF516_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEX8109544.1"
                     /translation="MRTTVTLEPDLAKKVKALAHRRKLSFKQALNEVIRRGLTSPDRQ
                     EAQTRFTVQPHAGGFRPGIDPGKLNQLVDQLEVEDFIAKAQR"
     gene            1442..>2059
                     /locus_tag="VF516_17560"
     CDS             1442..>2059
                     /locus_tag="VF516_17560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109545.1"
                     /translation="MRIPGLSRRWLIAGAISLGVVLGATIGLAVVYPRVGAWMLRTRV
                     GGKLAQRLGRGIRFGAIDVAIGHAVMRDVEIRGPLDGDTPLVHIDQIDVDFDGWRSLF
                     GTVELGAARLDGVLVTIHRDAAGQDNVRDVLDRLRVKGEAGGGGPGLRPASITVTHGK
                     LLANDELTGATGLVADADARWTPDRLVAHVRGVTATTLNAPRAAAA"
BASE COUNT          308 a          670 c          747 g          334 t
ORIGIN      
        1 tgcttcgacg acgagtcgat cgcgatctgc gaggaccggc gcgcccagct gacccgtcgc
       61 cgcgggctgg tcgtgcaggg cctgaaggcg atcggcctcg acgttccggt cgtgcccgac
      121 ggcgcgttct acgtctacat caacatccgc tcgaccggtc tggacgcgat ggcgttctgc
      181 cagcgggtcc ttcacgaagc gcacgtcgcc ttgacgccgg gttacgattt cggccacgcg
      241 ctcgccagtc agcatgtccg gctgtcgtac gccgcggcag aggccgatct cgtcgaggcg
      301 ctgcaacggc tcggcgcggt catggcgcgc ctgtagcacg tgacgcggcg gaactgctcg
      361 ctggacgacc gtgaccggcg ccgcgaatga tggcatgaac ctgcaggcgc cccaacaacg
      421 atcgtggaac aactgcctct ttaccattca tgattggaca gcgcagacgt gcagtccctg
      481 gctacagcgg gttgcgccag cgcaggccgg ggaagcgagc gaagtcgttg tcgttggagt
      541 ggagctcgca ctggtgctcg atcgccagtg cagcgaggtg ggcgtcggtg gtgaggttgc
      601 cagcggtgcc gacggctgac agcaggcggt cgagcagatc gaggtgctgc ggcccgggct
      661 ccaggtgctc cacgttcggt tgggcgaacc aggcacgtat ctgcgcgaac gcgacggtgg
      721 gcgacatcgg gtggggcaaa acgaagcgat gggtcatgag ccggaggaaa ccgagcacga
      781 cgacccaggg gatcgcgatc ggctccgtgc cgttgagaag tccttcccac caggcacgcg
      841 cgactgcgtg gtggggagca tcgctgttgt aggcgtagac cacgaggttg acgtccggaa
      901 ggatcaccgc tgtgccttcg cgatgaagtc ctcgacctcc agttgatcga cgagctggtt
      961 gagcttgcct ggatcgatac cgggtcggaa cccacctgcg tgcggctgga ccgtgaacct
     1021 ggtctgcgcc tcctgccggt ccggcgaggt cagcccgcgc cggatcacct cgttcagtgc
     1081 ttgcttgaac gacagcttgc ggcgatgagc cagcgccttg accttcttgg ccaaatcggg
     1141 ctccagggtg accgtggtcc tcatcgaggc atcatgatgc ctcaagggta tgctgtcaag
     1201 ctgcctcccc aggtcagcgc ggatgctgct ctcggacagc gccgtgcctg gcccgagctc
     1261 gacgctgctg gggcagctgc gatccatggc cgtgcgccgc cggcgcagcc tgtgaccggc
     1321 gtcgcgccgc gccgccggcg aggctgagcg atcacgcgca gttgccgcag ttgacccaga
     1381 tttaccccca cctcccgtcg gtcgaatcat ctgagcgggc tcgttgttgt atacgagcca
     1441 tatgcggatc ccggggctgt ctcgacgctg gctgatcgcc ggcgcgatca gcctcggcgt
     1501 cgtgctgggt gcgacgatcg gactggcggt ggtgtacccc cgggtcgggg cgtggatgct
     1561 ccggacccgg gtcggcggca agctggcgca gcggctcggc cgcggcatcc ggttcggcgc
     1621 gatcgacgtc gcgatcgggc atgcggtgat gcgcgacgtc gagatccgcg ggccgctcga
     1681 cggcgacacg ccgctggtgc acatcgacca gatcgacgtc gacttcgacg gctggcgatc
     1741 gctgttcggc acggtcgagc tcggcgcggc gcggctcgac ggcgtgctgg tgacgatcca
     1801 ccgcgacgcg gccgggcagg acaacgtgcg cgacgtgctc gaccggctgc gcgtcaaggg
     1861 cgaggcgggc ggtggcgggc ccgggctgcg gccggcgtcg atcacggtga cccacggcaa
     1921 gctgctcgcc aacgacgagc tgaccggcgc gaccgggctg gtcgccgacg ccgacgcgcg
     1981 gtggacaccg gaccggctgg tcgcgcacgt ccgcggagtc accgcgacca cgctcaacgc
     2041 accccgggcg gcggcggcg
//
LOCUS       DASVOR010000901         2554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67359,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000901 DASVOR010000000
VERSION     DASVOR010000901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67359"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..909)
                     /locus_tag="VF516_17565"
     CDS             complement(<1..909)
                     /locus_tag="VF516_17565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109546.1"
                     /translation="MKIANLLICCAVVAGSLAAPAVLHAQPVDAPNIAGNEDMPWNKG
                     VPQATRAAARGVFLEGNRLFKIPLFSRAVEKYSEALDQWKHPAFYFNLAIAQINLGQW
                     LDARDNLERAMKYGPDPLRADRFDEAKKQLVEVERHLGRIRVRCPTPGAEVTLDAVTL
                     FTGPGQREIWVTASPHEVTAKKPEYGTQARRVPVAAGAQETVDLSLRKLVEDRPWATW
                     KPWAVVAAGAGVAAASGVLHGFSAHNFSAFDSDFAKLSCAAMGCKQDQIDPQLSSRLS
                     RAQQEQRIAVGGYIAGGALLAAGAVLV"
     gene            complement(941..>2554)
                     /locus_tag="VF516_17570"
     CDS             complement(941..>2554)
                     /locus_tag="VF516_17570"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8109547.1"
                     /translation="ALTVSGDLSQQNQLVARLYAEPGYLGAALRSRARSRGQHILLFV
                     DQFEELYTQVADPRERLAFAACLAGVCDDATTPLRLVLSLRSDFLDYVAEAPALMAEL
                     TRGLFFLTPPNRDGLREALSQPAEMAGYQFEDAAMIESMLDHLQSTPGALPLLQFAAS
                     QLWETRDRDRRLLTAESYHRLGGIAGALASHADAVIAECTPREQSLVRALFLRLVTPE
                     RTRAIVPVNELYELSAEPGEIHRVVDRLVRSRLLVGQTTTNASGGTVGGTVEIVHESL
                     ITGWPLLRRWLDETQEDAAFLDQLRNAAKQWQIRGYAKDLLWRGEAMQEARHWYSRYR
                     GQLPDLQRAYLNAVFALSVKATRRKRLALIGSMAFLSLLVVAAGVALYTIRAAQQEAT
                     AQAQRVAQQLALTQTAEKAAKTAEATANTEREKAVAARNDLASKNADLIAAIDEAKRA
                     QQAAEQARQDAEQARLRAEKSKRQERRSRHKATELAAAAEAAADEARRAKDKVEALLD
                     QEKKRVDELEKQSRGVKIIPDVAVHDPGR"
BASE COUNT          311 a          905 c          952 g          386 t
ORIGIN      
        1 caccagcacc gcgccggcgg cgagcagcgc gccgcccgcg atgtagcccc cgacggcgat
       61 ccgctgctcc tgctgggccc ggctcagccg cgagctcagc tgcgggtcga tctggtcctg
      121 cttgcacccc atcgccgcac aggacagctt ggcgaagtcg ctgtcaaagg cgctgaagtt
      181 gtgggcggag aacccgtgca gcacgccgct cgcggccgcg acgcctgcgc cggcggccac
      241 gaccgcccac ggcttccagg tcgcccacgg ccgatcctcg accagcttgc gcagcgacag
      301 atcgacggtc tcctgcgcgc cggccgccac ggggacgcgc cgggcctgcg tcccgtactc
      361 gggcttcttc gccgtgacct cgtgcgggct cgccgtgacc cagatctcgc gctggcccgg
      421 gccggtgaac agcgtgacgg cgtcgagcgt gacctcggcg cccggggtcg ggcagcggac
      481 gcggatccgg ccgaggtgac gctcgacctc gacgagctgc ttcttggcct cgtcgaagcg
      541 atccgcgcgc agcggatccg ggccgtactt catcgcccgc tcgaggttgt cgcgcgcgtc
      601 gagccactgg ccgaggttga tctgcgcgat cgccaggttg aagtagaagg cggggtgctt
      661 ccactggtcg agcgcctcgc tgtacttctc gaccgcgcgc gagaacagcg ggatcttgaa
      721 cagccggtta ccctcgagga acaccccgcg cgccgccgcg cgggtcgcct gggggacgcc
      781 cttgttccac ggcatgtcct cgttgccggc gatgttgggc gcgtccaccg gctgggcgtg
      841 gagcaccgcg ggcgcggcga gcgagccggc gaccaccgcg caacagatca gtagattggc
      901 aatcttcacg tgcaaccccc gtcggcgttc ggcggcccgg tcatcggccg gggtcatgga
      961 cggccacgtc cgggatgatc ttgacgccgc gtgactgctt ctcgagctca tcgacccgct
     1021 tcttctcctg gtcgagcagc gcttcgacct tgtccttcgc gcgccgggcc tcgtcggcgg
     1081 cggcctccgc cgcggcggcg agctcggtcg ccttgtgccg gctgcggcgc tcctggcgct
     1141 tggacttctc ggcgcgcagc cgggcctgct cggcgtcctg gcgcgcctgc tcggccgcct
     1201 gctgcgcgcg cttggcctcg tcgatcgcgg cgatcaggtc cgcgttcttg ctcgccaggt
     1261 cgttgcgggc cgcgaccgcc ttctcgcgct cggtgttggc ggtggcctcg gcggtcttgg
     1321 cggccttctc ggcggtctgg gtcaacgcga gctgctgcgc gacgcgctgc gcctgggcgg
     1381 tcgcctcctg ctgggccgcg cggatggtgt acagcgcgac gccggcggcg accacgagca
     1441 gcgacaggaa cgccatcgag ccgatgagcg ccaggcgctt gcggcgcgtc gccttgaccg
     1501 acagcgcgaa caccgcgttg aggtaggcgc gctgcaggtc gggcagctgg ccgcggtagc
     1561 ggctgtacca gtgcctggcc tcctgcatcg cctcgccgcg ccacaagagg tccttggcgt
     1621 agccccggat ctgccactgc ttggccgcgt tgcgcagctg gtcgaggaac gccgcgtcct
     1681 cctgggtctc gtcgagccag cgccgcagca gcggccagcc ggtgatcagc gactcgtgga
     1741 cgatctcgac ggtgccgccg accgtgccgc cgctcgcgtt ggtggtggtc tggccgacca
     1801 gcaggcgcga gcgcacgagg cgatcgacga cgcggtggat ctcgccgggc tcggccgaca
     1861 gctcgtagag ctcgttcacc ggcacgatcg cgcgggtgcg ctcgggggtg accagccgca
     1921 ggaacagggc gcgcaccagg ctctgctcgc gcggcgtgca ctcggcgatc acggcgtcgg
     1981 cgtggctggc gagcgcgccg gcgatgccgc cgaggcggtg gtagctctcg gcggtgagca
     2041 gccggcgatc gcggtcgcgg gtctcccaca gctggctggc cgcgaactgg agcagcggca
     2101 gggcgcccgg cgtgctctgc aggtgatcga gcatcgactc gatcatcgcg gcgtcctcga
     2161 actggtagcc ggccatctcg gcgggctggc tcagcgcctc gcgcaggccg tcgcggttgg
     2221 gcggggtcag gaagaacagg ccgcgggtga gctcggccat cagcgcgggg gcctcggcga
     2281 cgtagtcgag gaagtccgag cgcagcgaca gcacgagccg gagcggggtg gtcgcgtcgt
     2341 cgcacacgcc ggccaggcac gccgcgaacg cgagccgctc gcgcgggtcg gcgacctggg
     2401 tgtagagctc ctcgaactgg tcgacgaaca gcaggatgtg ctggccgcgg ctgcgcgccc
     2461 ggctgcgcag cgcggcgccg aggtagccgg gctcggcgta gagccgcgcg acgagctggt
     2521 tctgctgcga gaggtcgccc gacaccgtga gcgc
//
LOCUS       DASVOR010000902         4845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_213694,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000902 DASVOR010000000
VERSION     DASVOR010000902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213694"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VF516_17575"
     CDS             <1..319
                     /locus_tag="VF516_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109548.1"
                     /translation="ERRHDQTPHPSIDEWREFIALWAAYHAAVTAGGLELRRLAFPNA
                     FSKGSNMAAWLWNKREEYALSHAISRWLLDEALAVGDAEAIELGHRNCALAVPTRLGR
                     IQS"
     gene            377..2584
                     /locus_tag="VF516_17580"
     CDS             377..2584
                     /locus_tag="VF516_17580"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8109549.1"
                     /translation="MTTRSTVAALGALLSWIAACSNRTPPPLAPEAPANGPAEYQAPP
                     PRPAVPARDYPDSRRDDVVDRIHGAAVPDPYRWLEDPGQPEVQAWMKAQDGYARERLA
                     RLPGRDALAARFAEVFYYDEIDAPEHRAGRYFYTRKHRDREKAIVHWRQGERGAEQVL
                     LDPNTWSADGSAGLRGWWPSATGRYVAFNRTEHNADEATLGVIDVATGQALRDTIPGT
                     KYGRASWLGDLGFYYVWVPPPGPEVPVADRPGFAELRYHALGSDPARDPVVREATRDP
                     QTFVGGTVSRDGHWLFAEIRHGWNSTDLYFQDLRQRGKGWTALVEGVPAVFAVEDFRD
                     VFYVRTNDGAPRYRIFAVDPRHPARADWKPLVAETDATLQSFAVIGGSLVLDYLRSAA
                     SEVEVHDLHGALVRKIDIPPLGNASPMRGLPDEDDGYLSYTSFTEPHVVYKISIRRGS
                     VTEWARVALPIDTAQLTTEQVRYPSKDGTEITMFLVHRKDAVKDGKTPVYLTGYGGFS
                     QPVLPGFVSSRSSWAAHAVWMEHGGMVAVPNLRGGNEYGEQWHKAGMLLEKQHVFDDF
                     LAAARWLITSGWTSPDHLAISGGSNGGLLVGAALTQAPELFKAVVCEVPLLDMVRYHR
                     FGSGKTWAPEYGSAEDPAQFQALLAYSPYHHIQRGVRYPAVLMSSADHDDRVDPMHAR
                     KFTAALQWDSAGEGPVWLRIQANAGHAGVDQVKQKIEQNADTYAFLMWQLGMK"
     gene            2678..3400
                     /locus_tag="VF516_17585"
     CDS             2678..3400
                     /locus_tag="VF516_17585"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8109550.1"
                     /translation="MTEPARRRATYQDVLDAPDDKIAEVVNGVLHLSPRPRIQHQSVT
                     SVLGAVLLTRFQLGRGGPAGWALLDEPELHLGGDILVPDIAGWRVERLPVDMDEDEGA
                     AFLTIAPDWICETLSRSTQRLDRGDKMASYAAAGVKHAWLVHPIRRTLEVFRLYRRRW
                     KTLATHHDHERIRAEPFEALELDLALLWRRLAALPPRRDRACEPTAIYRPDGLHVLEY
                     THASDDAYDLDEPCEVGAAYHP"
     gene            complement(3414..3896)
                     /locus_tag="VF516_17590"
     CDS             complement(3414..3896)
                     /locus_tag="VF516_17590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8109551.1"
                     /translation="MKITFRKATRGDSAVAMQLVDRALRDYGLYAVPEAGDIDLTDLE
                     QHYDARGGCFELIEAGGEVVGVVGWRPAGDGVMELKKLYLASSARGHGLGRRAAERMI
                     AAARAAGARAIVLETATRLTEANALYRRLGFVPVQGAAAASFATLTDQCDLAYRLELV
                     "
     gene            3996..4655
                     /locus_tag="VF516_17595"
     CDS             3996..4655
                     /locus_tag="VF516_17595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEX8109552.1"
                     /translation="MFIGHYAVGLAAKRVEPRLSLAVLLAAPQVLDLVWPILVLAGIE
                     HVEVAPGDTAFTPLAFTSYPWSHSLVAALVWAAVMMWIVRSIAPDGWRPAAIAGALVV
                     SHWVLDVASHRADMPLWPGSVRLGLGLWQSVPATLAVEIAMYAIGAGLYLTATRARDR
                     LGRTLPWILIVLLLGIYLGNVFDPPPPSGRIVAVIALALWLVPLAGWWIERHRPPRSA
                     R"
     gene            complement(4644..>4845)
                     /locus_tag="VF516_17600"
     CDS             complement(4644..>4845)
                     /locus_tag="VF516_17600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109553.1"
                     /translation="TSCASCHREDECLTCHSAQPGAPHVSPHPANWRGSAQCRALDRG
                     NRRMCLRCHVSQAELGCDWSAR"
BASE COUNT          673 a         1807 c         1663 g          702 t
ORIGIN      
        1 cgagcgccgc cacgatcaga cgccgcaccc gtcgatcgac gagtggcgtg agttcatcgc
       61 gctgtgggcg gcctaccacg ccgcggtcac cgcgggcggc ctggagctgc gccggctcgc
      121 gttccccaac gcgttcagca agggcagcaa catggccgcg tggctgtgga acaagcgcga
      181 ggagtacgcg ctgtcccacg cgatctccag gtggctgctc gacgaggccc tcgcggtcgg
      241 cgacgccgag gcgatcgagc tcggccaccg caactgcgcg ctcgccgtgc cgacccggct
      301 cggccggatc cagtcgtagc agcggccgtg tgatcgcgcc gagccggccc ccccgcggtt
      361 ctggtatcac cgcggcatga cgacccgatc gaccgtcgcc gcgctcggtg ccctcttgag
      421 ctggatcgcc gcctgctcga accgcacgcc gccgccgctc gcccccgagg ccccggccaa
      481 cggccccgcg gagtaccagg cgccgccgcc caggccggcg gtcccggcgc gcgactaccc
      541 ggacagccgg cgcgacgacg tcgtcgaccg gatccacggc gccgcggtgc ccgatccgta
      601 tcgctggctc gaggatccgg gccagcccga ggtccaggcc tggatgaagg cgcaggacgg
      661 ctacgcgcgc gagcgcctgg ccaggctgcc cggccgcgac gcgctcgccg cgcggttcgc
      721 cgaggtgttc tactacgacg agatcgacgc gcccgagcac cgcgccggcc gctacttcta
      781 cacgcgcaag caccgcgacc gcgagaaggc gatcgtccac tggcggcagg gcgagcgcgg
      841 cgccgagcag gtcctgctcg accccaacac ctggtcggcg gacggctcgg ccgggctgcg
      901 cggctggtgg ccgagcgcga ccggccgcta cgtcgccttc aaccgcaccg agcacaacgc
      961 cgacgaggcg acgctcggcg tgatcgacgt cgccaccggc caagcgctgc gcgacaccat
     1021 ccccggcacc aagtacggcc gcgcgtcgtg gctgggcgac ctcggcttct actacgtctg
     1081 ggtcccgccg cccggtcccg aggtcccggt cgccgatcgc cccggcttcg ccgagctgcg
     1141 ctaccacgcg ctcggcagcg accccgcccg ggacccggtg gtccgcgagg cgacccgcga
     1201 tccgcagacc ttcgtcggcg gcacggtgtc gcgcgacggc cactggctgt tcgccgagat
     1261 ccggcacggc tggaactcga ccgacctcta cttccaggac ctgcgccagc gcggcaaggg
     1321 ctggacggcg ctggtcgagg gcgtccccgc ggtgttcgcc gtcgaggact tccgcgacgt
     1381 gttctacgtg cggaccaacg acggcgcgcc gcgctaccgg atcttcgcgg tcgatccgcg
     1441 ccacccggcg cgcgccgact ggaagccgct cgtcgccgag accgacgcca cgctgcagtc
     1501 gttcgcggtg atcggcggca gcctcgtgct cgactacctg cgcagcgccg ccagcgaggt
     1561 cgaggtccac gacctgcacg gcgccctcgt ccgcaagatc gacatcccgc cgctcggcaa
     1621 cgccagcccg atgcgcgggc tgcccgacga ggacgacggc tacctgtcgt acacctcgtt
     1681 caccgagccg cacgtggtct acaagatctc gatccggcgc ggcagcgtca ccgagtgggc
     1741 gcgcgtcgcg ctgccgatcg acaccgcgca gctcaccacc gagcaggtcc gctatccgtc
     1801 gaaggacggc accgagatca cgatgttcct ggtccaccgc aaggacgcgg tcaaggacgg
     1861 caagaccccc gtgtacctga ccggctacgg cgggttcagc cagccggtgc tgcccggctt
     1921 cgtgtcgtcg cgctcgtcgt gggcggccca cgcggtgtgg atggagcacg gcggcatggt
     1981 cgccgtcccc aacctgcgcg gcggcaacga gtacggcgag cagtggcaca aggccggcat
     2041 gctgctcgag aagcagcacg tgttcgacga cttcctcgcc gcggcgcgct ggctgatcac
     2101 cagcggatgg acctcgcccg accacctcgc gatcagcggc ggctcgaacg gcggcctgct
     2161 ggtcggcgcc gcgctcaccc aggcccccga gctgttcaag gccgtcgtct gcgaggtccc
     2221 gctgctcgac atggtgcgct accaccggtt cggctcgggc aagacctggg cccccgagta
     2281 cggcagcgcc gaggaccccg cgcagttcca ggcgctgctc gcctactcgc cgtatcacca
     2341 catccagcgc ggcgtccgct acccggcggt cctgatgtcg agcgccgatc acgacgaccg
     2401 ggtcgatccg atgcacgcgc gcaagttcac cgccgcgctg cagtgggaca gcgccggcga
     2461 gggcccggtc tggctgcgca tccaggccaa cgccggccac gccggcgtcg atcaggtcaa
     2521 gcagaagatc gagcagaatg ccgatacgta tgcattcctg atgtggcaac tgggcatgaa
     2581 atgatatcga cgctgcggca tcgatacagc gacagcgtcg atgctcgcat gatttcctga
     2641 tctgactgtc agtgtcccct gctagggtgt cctggccatg accgagccag ctcgccgtcg
     2701 cgcgacttat caggatgtcc tcgatgcccc ggacgacaag atcgccgagg tcgtcaacgg
     2761 tgtactccac ctgagcccgc gacctcgcat ccagcatcag tcggtgacga gcgttttggg
     2821 cgcggtgctg ctgacccggt tccagctcgg ccgtggtggc cccgccggct gggccctcct
     2881 cgacgagccc gagctgcacc tcggaggcga catcctggtg ccagatattg ccggctggcg
     2941 tgtcgagcgg ctgcccgtgg acatggacga ggacgagggc gcggcgttcc tgaccatcgc
     3001 accggactgg atctgcgaga cgctgtcccg ctcgacgcag cggctcgacc gcggcgacaa
     3061 gatggcgagc tatgcggctg cgggcgtgaa gcacgcctgg ctggtccacc cgatccgccg
     3121 gaccctggag gtgttccggc tgtaccggcg ccggtggaag acccttgcga cccatcacga
     3181 ccacgagcgg atccgcgccg agccgttcga ggcgctggag ctcgatctgg cgctgctgtg
     3241 gcgccggctc gcggcattgc cgccgcgccg ggaccgcgcc tgcgagccga ccgccatcta
     3301 ccgccccgac gggctccacg tgctcgaata cacccacgcg tccgacgacg cctacgatct
     3361 cgacgagccc tgcgaggtcg gcgcggccta ccacccgtag gcgaagcccg agctcacacc
     3421 agctcgaggc ggtacgcgag gtcgcactgg tcggtcagcg tcgcgaacga cgccgcggcc
     3481 gcgccttgca ccgggacgaa gccgagccgc cgatacagcg cgttggcctc ggtcagccgg
     3541 gtcgcggtct cgagcacgat cgcgcgagca ccggcggcgc gagccgctgc gatcatgcgc
     3601 tcggccgcgc gccggcccag gccgtggccg cgcgcgctcg atgccaggta cagcttcttg
     3661 agctccatca cgccgtcgcc cgccgggcgc cacccgacga cgccgacgac ctccccgccg
     3721 gcctcgatca gctcgaagca gccgccgcgc gcgtcgtagt gctgctcgag atcggtcagg
     3781 tcgatgtccc cggcctcggg caccgcgtac agcccgtagt cgcgcagcgc tcgatcgacc
     3841 agctgcatcg cgaccgcgct gtcaccgcgc gtggccttgc ggaacgtgat cttcatcgcc
     3901 accatggtag cgcgtcgtgc tcgtgtctcg tcgcgctgca ggcgcagatc gagagattga
     3961 cactctggcc cgccgcgccc gatagtgcgc ctgcgatgtt catcggacac tacgcagtcg
     4021 ggctcgcggc caagcgcgtg gagccgcggc tctcgctcgc ggtcctgctc gcggcgccgc
     4081 aggtcctcga tctggtgtgg ccgatcctgg tgctcgccgg catcgagcat gtcgaggtcg
     4141 cgcccggcga taccgcgttc acgccgctcg cgttcaccag ctacccgtgg tcgcacagcc
     4201 tggtcgccgc gctcgtgtgg gccgccgtga tgatgtggat cgtccgctcg atcgctccgg
     4261 acgggtggcg tccggccgcg atcgccggcg cgctcgtggt cagccactgg gtgctcgacg
     4321 tcgcgagtca ccgcgccgac atgccgctgt ggccgggcag tgtgcggctc gggctcggcc
     4381 tctggcagtc ggtgccggcg acgctggcgg tcgagatcgc gatgtatgcg atcggcgccg
     4441 gcctctacct gaccgcgacc cgcgcccggg accgtctcgg ccggacgcta ccgtggatcc
     4501 tgatcgtcct cttgctgggg atctatctcg gcaacgtatt cgaccctccg ccaccgtcgg
     4561 gccggatcgt cgcagtcatc gcgctcgcgc tgtggctcgt gccgctcgcc ggatggtgga
     4621 tcgagcgcca tcggccgccg cgttcagcgc gctgaccagt cgcagccgag ctcggcctgg
     4681 ctgacgtgac agcgcaggca catccgccgg ttgccgcggt cgagcgcccg gcactgcgcg
     4741 ctgccgcgcc agttggcggg gtgcggcgag acgtgcggcg cgccgggctg ggcgctgtgg
     4801 caggtcaggc actcgtcctc gcggtggcac gacgcgcagc tggtg
//
LOCUS       DASVOR010000903         1828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_97252,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000903 DASVOR010000000
VERSION     DASVOR010000903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97252"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1828
                     /locus_tag="VF516_17605"
     CDS             <1..>1828
                     /locus_tag="VF516_17605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109554.1"
                     /translation="LVASLSAGPAAHAQPGATDPQPPAGEPVPGAPGTTSEPGPSGVP
                     VAPPNGPGVGRPVRPPPVSTSPIQLSPREKNELKNVEAEHERFVAAANEHDARLRAIA
                     RREYDARASELQKRYAERIARAEADRGRRHTDTIALLEKFLKDHPDHEQFTPDAMFRL
                     ADLYIDKADAEIEAQLAAQESAGAPPEGAPDAAALTADYTPSVRLWQDILTRFPAYRQ
                     TPSTLYLLAYYGKAKDERRSLQVFLALACANKYKWNELPSKPPTRAEALKRIERKDLR
                     DPYADCTPYPGADTELVRHAWVRGVADYHFTIPGELDEAIAAYLKVANGGNDSKLYAE
                     SLYKLAWSYYKRDRLVDSIRRFDESVKLYDSIVAAGNTPPLELRDESIQYISVAFTDP
                     WEGETESNPSRAFDRAKAFYQGRENEPHVRDVWVAMGKAFTELQAWDQAVDSYRIAIG
                     PPWELNPANPVVHQEIVNVFEAKGDKLAADAAAAELATRYAPGTPWYQANEKDREAME
                     NQRRIAERALYAATRNTHSAATQLRKDYEATGKKDPAAKQEYLAMYAKAVDLYRTFIA
                     TYAESDYIYEFSFLEGEALYWSERYPEAIAQYKWVRDHRDLGT"
BASE COUNT          322 a          700 c          606 g          200 t
ORIGIN      
        1 ctcgtcgcat cgctctccgc gggccccgcc gcgcacgccc agcccggcgc gaccgatccg
       61 cagccaccgg ccggcgagcc ggtgccgggc gccccgggga cgacgtcgga gcccggcccg
      121 tccggcgtcc cggtcgcgcc ccccaacggt ccgggcgtcg gacgcccggt gcggcccccg
      181 ccggtcagca ccagcccgat ccagctgtcg ccccgggaga agaacgagct caagaacgtc
      241 gaggccgagc acgagcggtt cgtcgccgcc gccaacgagc acgacgcccg gctgcgcgcg
      301 atcgcccgcc gcgagtacga cgcgcgcgcg tccgagctcc agaagcgcta cgccgagcgg
      361 atcgcgcgcg cagaagccga ccgcggccgg cgccacaccg acacgatcgc gctgctcgag
      421 aagttcctca aggaccaccc ggaccacgag cagttcacgc ccgacgcgat gttccggctc
      481 gccgacctgt acatcgacaa ggccgacgcc gagatcgagg cccagctcgc cgcgcaggag
      541 agcgccggcg cgccgcccga gggcgccccc gacgccgccg cgctgaccgc ggactacacg
      601 ccatcggtcc ggctgtggca ggacatcctg acccggttcc cggcctaccg gcagacgccg
      661 tcgacgctgt acctgctcgc ctactacggc aaggccaagg acgagcggcg ctcgctccag
      721 gtgttcctcg cgctggcgtg cgccaacaag tacaagtgga acgagctgcc gagcaagccg
      781 ccgacccgcg ccgaggcgct caagcggatc gagcgcaagg acctgcgcga tccgtacgcc
      841 gactgcacgc cgtaccccgg cgccgacacc gagctggtgc gccacgcctg ggtccgcggc
      901 gtcgccgact accacttcac gatcccgggc gagctcgacg aggcgatcgc cgcgtacctc
      961 aaggtcgcca acggcggcaa cgactccaag ctgtacgccg agtcgctgta caagctggcg
     1021 tggagctact acaagcgcga ccggctggtc gactcgatcc gccggttcga cgagagcgtc
     1081 aagctctacg actcgatcgt cgccgccggc aacacgccgc cgctcgagct gcgcgacgag
     1141 tcgatccagt acatctcggt cgcgttcacc gacccgtggg agggcgagac cgagagcaac
     1201 ccgtcccggg cgttcgaccg cgcgaaggcg ttctaccagg gccgcgagaa cgagccccac
     1261 gtccgcgacg tctgggtcgc gatgggcaag gcgttcaccg agctccaggc ctgggaccag
     1321 gcggtcgaca gctaccggat cgcgatcggc ccgccgtggg agctcaaccc ggccaacccg
     1381 gtcgtccacc aggagatcgt caacgtgttc gaggccaagg gcgacaagct cgcggccgac
     1441 gccgccgccg ccgagctcgc gacccgctac gcgccgggca cgccgtggta ccaggccaac
     1501 gagaaggacc gcgaggcgat ggagaaccag cgccggatcg ccgagcgcgc gctgtacgcg
     1561 gcgacccgca acacccactc ggccgcgacc cagctgcgca aggactacga ggcgacgggc
     1621 aagaaggacc cggccgccaa gcaggagtac ctcgcgatgt acgccaaggc ggtcgacctg
     1681 taccgaacgt tcatcgcgac ctacgccgag tcggactaca tctacgagtt cagcttcctc
     1741 gagggcgagg cgctgtactg gagcgagcgc taccccgagg cgatcgcgca gtacaagtgg
     1801 gtccgcgatc accgcgacct cggcacca
//
LOCUS       DASVOR010000904         2729 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_476853,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000904 DASVOR010000000
VERSION     DASVOR010000904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476853"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VF516_17610"
     CDS             complement(<1..869)
                     /locus_tag="VF516_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109555.1"
                     /translation="MDTRTLLRERLRRSRDWSAAIDDLEKEAEASGSKPEQSERLFEL
                     AALVEDVIPERERALGLYQRAWKLHPDNLKALSRAREVYGEIGRFEMVAKLGEMELRS
                     PAAPTNLAAIVGEALLDSGQKDRAIPILQRALEIDPDSVRVKDALAAASYDPEFWVDE
                     FDRLTDEADRFDEATAVRMLVRAARILRIEAPEDERLEELLKRILAKDIDEPSTNFLY
                     ETLLAGTGRWDELEAHHRRRADRAPDHRKKIEALRTFGLEWVQRFKDRDRGARFFDAA
                     LRATVSNGVVPMRS"
     gene            complement(1161..2183)
                     /locus_tag="VF516_17615"
     CDS             complement(1161..2183)
                     /locus_tag="VF516_17615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8109556.1"
                     /translation="MKLVGIAALALLAVLPGCPNEGRNDSITAANEGARALGQKQYET
                     AIAAYLKATDKWDKNDQAWYGLGGAYAGRQDWAKAADAMAHAVQLVPEQPMYQLYYGR
                     YLYEKTVKTATEDQARHENKKPEEFTPDLTAVSFELPLQHFQEAIKLSPDLWRAHYYI
                     GRIYRDTGKNKEAAEELTKALQAGAIDPEPWVALAELYRRWDYTDQAIQVAEAGTAVV
                     SGSREKSQLWYEVGMGYDDKRLNDKAIDAFTKALEVKRDYHAARFQRGQAYFRKQDYA
                     NAKRDLEEFSKTGGTSVQFEKQQASKLLVDIAAKSVVPGSTPVEQPSPEDVVKKGKAG
                     KGKRGH"
     gene            complement(2439..>2729)
                     /locus_tag="VF516_17620"
     CDS             complement(2439..>2729)
                     /locus_tag="VF516_17620"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HEX8109557.1"
                     /translation="LDKAIIRRYIKRNIQKIQYCYEKQLLAKPTLAGTVSAQFFITPN
                     GTVAQSTGSGLDPEVASCVAEVIKNIEFPKPKGGGGVQVNYPFTFHSSGGGG"
BASE COUNT          367 a          961 c          911 g          490 t
ORIGIN      
        1 gagcgcatcg gcaccacgcc gttggacacc gtggcgcgca gcgcggcgtc gaagaaccgc
       61 gccccgcgat cgcggtcctt gaaccgctgc acccactcga gcccgaacgt gcgcagcgcc
      121 tcgatcttct tcctgtgatc cggggcgcgg tcggcgcggc ggcggtggtg ggcctcgagc
      181 tcgtcccacc ggccggtgcc ggcgagcagg gtctcgtaca agaagttcgt gctcggctcg
      241 tcgatgtcct tggccaggat ccgcttgagc agctcctcga gccgctcgtc ctcgggcgcc
      301 tcgatccgca ggatccgcgc cgcgcgcacc agcatgcgca ccgcggtcgc ctcgtcgaac
      361 cggtcggcct cgtcggtgag ccggtcgaac tcgtcgaccc agaactcggg gtcgtagctc
      421 gccgcggcga gcgcgtcctt gacccggacc gagtccggat cgatctccag cgcgcgctgc
      481 aggatcggga tcgcccggtc cttctggccg ctgtcgagca gcgcctcgcc gacgatcgcg
      541 gccaggttgg tcggcgcggc ggggctgcgc agctccatct cgccgagctt ggcgaccatc
      601 tcgaaccggc cgatctcgcc gtagacctcg cgcgcgcgcg acagcgcctt cagattatct
      661 ggatggagct tccaggcgcg ctggtacaga ccgagggcgc gctcccgctc ggggatcacg
      721 tcctcgacga gcgcggcgag ctcgaacaac cgctccgact gctcgggctt ggagcccgac
      781 gcctccgcct ctttctcgag atcatcgatg gctgccgacc aatcacgcga ccggcggagc
      841 cgttcccgaa gcagtgtgcg cgtatccatc tgcggtctat cctcccgcaa ctcaatagga
      901 aaccccgccc ataacctgcg aatttttata tcacgggtga cctgccgcgt cgccgctcgt
      961 gccggttttt cgctggctcc gccaccgccg gtctccgacg gaaggcgcgc tgtgtggggg
     1021 ccagtgcgcc gacgcacgag cgccaggacc gctcgcccgc gcgccgtggc tcacctgccc
     1081 gggggccggt gggccggcgg ccgagaattt cacggaaaac cccggtaggc tcccgcgccg
     1141 gccgggggcc gcctggcccg ctagtggccc cgcttgccct tgccggcctt gcccttcttg
     1201 accacgtcct cgggcgacgg ctgctccacc ggcgtcgagc ccggcaccac cgacttggcg
     1261 gcgatgtcga ccagcagctt cgacgcctgc tgcttctcga actggaccga cgtgccgccg
     1321 gtcttggaga actcctcgag gtcgcgcttg gcgttggcgt agtcctgctt gcggaagtag
     1381 gcctggccgc gctggaaccg ggcggcgtgg taatcgcgct tgacctccag cgccttggtg
     1441 aacgcgtcga tcgccttgtc gttgagccgc ttgtcgtcgt agcccatgcc gacctcgtac
     1501 cagagctggc tcttctcgcg gctaccggac acgaccgcgg tcccggcctc ggcgacctgg
     1561 atcgcctggt cggtgtagtc ccagcggcga tacagctcgg cgagcgcgac ccacggctcg
     1621 gggtcgatcg cgccggcctg cagcgccttg gtcagctcct cggcggcctc cttgttcttg
     1681 ccggtgtcgc ggtagatccg gccgatgtag tagtgcgcgc gccacaggtc ggggctgagc
     1741 ttgatcgctt cctggaagtg ctggagcggc aactcgaagc tgaccgcggt cagatcaggc
     1801 gtgaactcct cgggcttctt gttctcgtgg cgcgcctggt cctcggtggc ggtcttcacg
     1861 gtcttctcgt agaggtaccg gccgtagtac agctggtaca tcggctgctc cggcacgagc
     1921 tggaccgcgt gcgccatggc gtcggccgcc ttggcccagt cctgcctgcc ggcgtaggcc
     1981 ccgcccaggc cataccaggc ctggtcgttc ttgtcccact tgtcggtcgc cttcaggtac
     2041 gccgcgatcg cggtctcgta ctgcttctgg ccgagggccc gggcgccctc gttcgcggcg
     2101 gtgatcgagt cgttgcgccc ctcgttcggg caaccgggga gcacagcgag cagcgcaagg
     2161 gcggcgatcc cgacgagctt catgcccgca acggtaaaac gccgacgccc gccactcaag
     2221 cgccggccgt cggggaatcg cgcgctggcc gccgctccgc tcattcacac atcgtcggcc
     2281 gcgagcctcg atggagagcg gcaacgggtc ggcgtcgacg gtggggtcag acgtctacgt
     2341 accgcttcgc tgccgtcaac ctcgacggtg gggtcaaggt cgacgttcac gtcaacccca
     2401 tgttccctca aacttgtgac gaatcatcac agccgatgct agccgccgcc gcccgacgag
     2461 tggaacgtga acggatagtt gacctgcacg ccgccgccgc ccttgggctt ggggaactcg
     2521 atgttcttga tcacctcggc gacgcagctc gcgacctcgg gatcgaggcc gctgccggtc
     2581 gactgcgcga cggtgccgtt gggcgtgatg aagaactgcg cggacaccgt gccggcgagc
     2641 gtcggcttgg cgagcagctg cttctcgtag cagtactgga tcttctggat gttgcgcttg
     2701 atgtagcggc ggatgatcgc cttgtcgag
//
LOCUS       DASVOR010000905         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447203,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000905 DASVOR010000000
VERSION     DASVOR010000905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447203"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VF516_17625"
     CDS             <1..523
                     /locus_tag="VF516_17625"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEX8109558.1"
                     /translation="SVLLPDNDWQEVAPLLAVLACLSVFRPITWVLSAYLEAESKTNR
                     LMGLELAKIGLLIGGIVVLQPYGVRIASAAVGFAFAATAIAGVALVMREGPSPRRLAI
                     GFAQPLAACGVMAAAVWAVHTLVAPVAPPGVVLVAMIAVGTVAYVAAALVICRATVRD
                     LLSLVRRAMHRPS"
     gene            725..>3623
                     /locus_tag="VF516_17630"
     CDS             725..>3623
                     /locus_tag="VF516_17630"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8109559.1"
                     /translation="MRSFDWSRTPLGPVVHWPQSLRTAVSIMLDSAFAMVVAWGPEFV
                     FLYNDRYRPVLGATKHPGALGKAAKDIFPEAWGFLGPLFENTRNGEAVALDDILIPLE
                     RNGYLEDCYFTLSYSAIRDESGGVGGMLAVVAETTERVRGERRMVTLRDLAALGDARN
                     ADDACRRAETVLAGNPVDVPFSLLYLLQPERRGARLAAATGVMKHAAAAPGEIVLDDA
                     RWPLGEAYRERRTIVVDDLRARFGAMPGGPYPQPAHTALVLPMPRAGHDEPHGFFVAG
                     VSPRRALDDRYRTFFELARDHLVAALDKALAFEDSRKRTEALAEIDRAKTALFSNISH
                     EFRTPLTLMLGLLEDALARGSITPDDLGTAFRDPAEEAVAVSPAERAEPAPAPRARVL
                     VADDNADMRSYLRGVLAPHVAVDTACDGRAALEAARCAPPDLVLTDVMMPNLDGFGLM
                     RALRADERTRAIPVVLLSARAGDESRIEGLQAGADDYLVKPFSARELIARVRTHLELS
                     RLRREAELQSSRLNTLFEHAPAAICVLEGPDHVYRLMNPPCLEAIGHRPVIGQTIRQA
                     MPELEGQGIVEMLDRVYATGEQYIDSEVPIRLQRGAQLEQTYWNVVFQPYRDAEGNVE
                     GVMTFGFEITMQVRARQRAEALTRQLQASEAKAEAANRAKDEFLAVLGHELRNPLAPI
                     LTALQLMRLRGGDTGARERSIIERQAQHLVRLVDDLLDVSRITRGKIELKTKRVELGD
                     VVARAIEMTSPVLEQRQHRLGLHVPPCGLAVDGDPDRLAQIFSNLLTNAAKYTERGGR
                     ITVSAERCDDRIGIRVKDTGIGIAPEMLPRVFEMFVQEQQALDRSRGGLGLGLTIVRS
                     LVAMHGGTVEVHSEGRNRGSEFVVLLPAAGVRPPGGADEAAAPNGARRGAGAGRRILV
                     VDDNEDAAELLASALEVMGHTTRVAHDGPEALKAGAEFDPDLALLDIGLPVM"
BASE COUNT          491 a         1261 c         1338 g          533 t
ORIGIN      
        1 ctcggtgctg ctgcccgaca acgactggca ggaggtcgcg ccgctgctcg ccgtgctggc
       61 ctgcctgtcg gtgttccggc cgatcacctg ggtgctgtcg gcgtacctcg aggccgagtc
      121 gaagaccaac cggctgatgg ggctggagct ggccaagatc ggcctcttga tcggcggcat
      181 cgtcgtgctg cagccgtacg gcgtgcggat cgcgagcgcc gcggtcggct tcgcgttcgc
      241 cgcgaccgcg atcgccggcg tcgcgctcgt catgcgcgag gggccgtcgc cgcgccggct
      301 ggcgatcggc ttcgcccagc cgctcgcggc gtgcggcgtg atggccgcgg cggtgtgggc
      361 ggtgcacacg ctggtcgccc cggtcgctcc gcccggcgtg gtgctggtcg cgatgatcgc
      421 ggtcggcacg gtcgcctacg tcgcggcggc gctggtgatc tgccgcgcga ccgtgcgcga
      481 tctgctgtcg ctggtgcggc gcgcgatgca ccggccgagc tgacatccct gatcgggaat
      541 accacgaggt catcggcgct cgccgtgcgc cggttgcttg cgaacgaccc gcacacggct
      601 acctggtcgg tccggttgcc gtgcacggtg cctgtgggca tcctcggttg tgcacgacac
      661 gagcgcggca tcgaggccgg cgaacgctga ctgtctggcc ggcggcggcg agatgggtgc
      721 gctcatgcgg tcgttcgact ggtccaggac cccgctcggc ccggtcgtgc actggccgca
      781 gagcctgcgc accgcggtca gcatcatgct cgactcggcg ttcgcgatgg tggtcgcatg
      841 ggggccggag ttcgtgttcc tgtacaacga tcgctaccgg ccggtgctcg gagccacgaa
      901 gcatcccggc gcgctcggca aggcggcgaa ggacatcttc ccggaggcgt gggggttcct
      961 cggaccgctg ttcgagaaca cccggaacgg cgaggcggtc gcgctcgacg acatcctgat
     1021 cccgctcgag cgcaacgggt acctcgagga ctgctacttc acgctgtcat acagcgcgat
     1081 ccgcgacgag tcggggggcg tcggcggaat gctggcggtg gtcgcggaga ccaccgagcg
     1141 cgtccggggc gagcgcagga tggtcacgct gcgcgacctc gcggcgctcg gtgatgcgcg
     1201 caacgcagac gacgcatgcc ggcgggcgga gacggtcctg gccgggaacc cggtcgacgt
     1261 gccgttctcg ttgctctacc tgctccagcc ggagcgccgc ggcgcgcgcc tggcggcggc
     1321 gaccggcgtc atgaaacatg cagccgcggc tcccggcgag atcgtgctcg acgacgcgcg
     1381 ctggccgctg ggcgaggcct accgcgagcg ccggacgatc gtcgtcgacg atctgcgggc
     1441 gcggttcggc gcgatgcccg gaggtccgta cccacagccg gcgcacaccg cgctggtcct
     1501 gcccatgccg cgcgccggcc atgacgagcc gcacgggttc ttcgtcgccg gggtgagtcc
     1561 tcgccgcgcg ctcgacgatc gctatcgcac gttcttcgag ctggcgcgcg atcacctcgt
     1621 cgcggcgctc gacaaggcgc tcgcgttcga ggactcgcgc aagcgaaccg aggcgctcgc
     1681 cgagatcgat cgcgcgaaga ccgcgttgtt ctcgaacatc agccacgagt ttcgcacgcc
     1741 gctgacgctc atgctcggac tgctcgagga cgcgctcgcg cgcggctcga tcacgcccga
     1801 cgatctcggc actgcgtttc gcgatccggc ggaggaggcg gtcgccgtgt cgccggccga
     1861 gcgcgccgaa ccggcgccgg caccacgcgc ccgcgtgctg gtcgccgacg acaacgcgga
     1921 catgcgcagc tacctgcgcg gcgtgctcgc cccgcacgtc gcggtcgata cggcctgcga
     1981 cggccgggcc gcgctcgagg ccgcgcggtg cgcgcccccc gacctggtgc tcaccgacgt
     2041 gatgatgccg aacctcgacg ggttcggtct gatgcgggcg ctgcgcgcgg acgagcgcac
     2101 ccgggcgatc ccggtcgtgc tgctgtcggc gagggccggc gacgagtcgc gcatcgaggg
     2161 cttgcaggcc ggcgccgacg attacctcgt caagccgttc tcggcgcgcg agctgatcgc
     2221 gcgcgttcgc acccacctcg agctgtcgcg gctgcgccgc gaggccgagc tccagtcgag
     2281 ccggctcaac acgctgttcg agcacgctcc cgccgcgatc tgcgtgctcg aagggcccga
     2341 ccatgtctat cgcctcatga acccgccttg cctcgaggcg atcggccacc ggccggtgat
     2401 cggccagacg attcgccagg cgatgccgga gctggagggg cagggcatcg tggagatgct
     2461 cgatcgggtc tacgccaccg gtgagcagta catcgactcc gaggtgccga tccggttgca
     2521 gcgcggcgcg cagctcgaac agacctactg gaacgtcgtg ttccagccgt atcgcgatgc
     2581 cgagggcaac gtcgagggcg tgatgacgtt cgggttcgag atcacgatgc aggtgcgggc
     2641 gcgccagcgc gccgaggcgc tgacccgtca gctccaggcg agcgaggcga aggcggaggc
     2701 cgcgaaccgc gcgaaggacg agttcctcgc ggtgctcggt cacgagctgc gcaacccgct
     2761 ggccccgatc ctcaccgcgc tgcagctcat gcggctgcgc ggcggcgaca ccggcgccag
     2821 ggagcgctcg atcatcgagc gccaggccca gcacctggtc cggctcgtcg atgatctgct
     2881 cgacgtctcg cggatcacgc gcggcaagat cgagctcaag accaagcgcg tcgagctcgg
     2941 cgacgtggtg gcgcgcgcca tcgagatgac gagcccggtg ctggagcaac gccagcatcg
     3001 cctcggcctg cacgtgccgc cgtgcggcct cgcggtcgat ggcgatccgg accgactggc
     3061 gcagatcttc tcgaacctgc tgaccaacgc cgccaagtac accgagcgcg gcggccggat
     3121 cacggtgtcc gccgagcggt gcgacgaccg gatcggcatc cgcgtgaagg acaccggcat
     3181 cgggatcgcg ccggagatgc tgccgcgggt gttcgagatg ttcgtgcagg agcagcaggc
     3241 gctcgatcgc tcgcgcggtg ggctcgggct cgggctcacc atcgtgcgca gcctggtcgc
     3301 gatgcacggc ggcacggtcg aggtgcacag cgaaggccgc aatcgcggct ccgagttcgt
     3361 cgtcctgctt cccgccgccg gggtccggcc gccgggggga gccgacgaag cggccgcgcc
     3421 gaacggcgca cgtcgcggcg ccggcgcggg gcgcaggatc ctggtggtcg acgacaacga
     3481 ggacgcggcg gagctgctgg ccagcgcgct ggaggtcatg ggccacacca cccgggtcgc
     3541 gcatgacggc ccggaggcgc tgaaggccgg cgccgagttc gatcccgatc tcgcgctgct
     3601 cgacatcggg ctgccggtga tgg
//
LOCUS       DASVOR010000906         2356 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_9579,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000906 DASVOR010000000
VERSION     DASVOR010000906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9579"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VF516_17635"
     CDS             <1..582
                     /locus_tag="VF516_17635"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8109560.1"
                     /translation="LFHYVQQHVAAQPLDQRPVFKAELEENYPIAELHGGAAAAFTLP
                     PPPDDAAYDVFVSYCQSAPDDRAWVTGALVPYLEGLGLRLCLENRDFRFGRPKLDEID
                     RAVTTSRYTLAVFTPAYLASAWADYQSVLAAYLAAESGEPRFVPLLRRPCQLALHDRM
                     TEALDVSRDPEVPAALQRLALALRQPPRPRLAG"
     gene            complement(622..1797)
                     /locus_tag="VF516_17640"
     CDS             complement(622..1797)
                     /locus_tag="VF516_17640"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8109561.1"
                     /translation="MKPDPAVLGLTTGDIGTCQVCSHPYRVLSTSPYRLARVKVEDRG
                     APQDLPVIRGDFAGKVRSDTGNARSRERVSGRYRSRNGGGMGPSKKAPAKKAHAKKAH
                     ARAMPSRRPPALWMPTKTPGKATAANRVQDVMLSATHPRAIRPGDSFLVEIFLHLRGA
                     GPRASARTAVSAERAVVRLRAGARLQIAIRPPPGITAGDPARSVVWRPPRSSVSFPLH
                     ASERIKSGTHILTVAVSTAGRRGVELARFDIALEIDRRAATTAPAVSTVLRRMPTSYF
                     ASYARRDRAKVMGRLSTIEAIGGDVFVDCLDLREGERWEDQIRRQVQERDGFLLFWSR
                     AARDSKWVGREWRHRLRHRGLDSITPNALESPRSCPPPRALARLQFGSRFLEATRRK"
     gene            complement(1896..>2356)
                     /locus_tag="VF516_17645"
     CDS             complement(1896..>2356)
                     /locus_tag="VF516_17645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109562.1"
                     /translation="LALDLDGATGRGHLDVVRDGGELHARGTSLPGVPAALDHAIAAD
                     GLLAPDAVLSRIVLWRPRLLPLGVGQTIELIEHDVGLASEIDDSERTFQVRCAEDRAG
                     GSGGAAGVTQVYEITPPRGPRSELVTDADGWPLELAIHDHGALLRLRRRP"
BASE COUNT          343 a          894 c          787 g          332 t
ORIGIN      
        1 ctgttccact acgtccagca gcacgtcgcc gcccagccgc tcgaccagcg cccggtgttc
       61 aaggccgagc tcgaggagaa ctacccgatc gccgagctcc acggcggcgc cgcggccgcg
      121 ttcaccctgc cgccgccgcc cgacgacgcc gcctacgacg tgttcgtgtc gtactgccag
      181 agcgcccccg acgaccgcgc ctgggtcacc ggcgcgctcg ttccgtatct ggaaggactc
      241 ggcctgaggc tgtgcctcga gaaccgcgac ttccggttcg gccggcccaa gctcgacgag
      301 atcgatcgcg ccgtcaccac gagccgctac accctcgccg tgttcacccc ggcctacctc
      361 gccagcgcgt gggccgacta ccagtccgtg ctcgccgcct acctcgccgc cgagtccggc
      421 gagccccgct tcgtcccgct gctccgccgg ccgtgccagc tcgccctcca cgaccgcatg
      481 accgaggccc tcgacgtcag ccgcgacccc gaggtccccg ccgcgctgca gcgcctcgcg
      541 ctcgccctgc gccagccgcc ccgcccccgc ctcgccggct gagcggcctc gtgcgacgtc
      601 agtgggcgag aaacgcgccg gtcatttgcg cctggtggcc tcgaggaagc gcgagccgaa
      661 ctggaggcgg gcaagtgccc gaggaggcgg acaggaccgc ggtgattcga gtgcgttggg
      721 cgtgatcgag tcgagcccgc ggtgacgcag ccggtgtcgc cactcgcgcc cgacccactt
      781 cgagtcccgc gcggcgcgcg accagaacag cagaaagcca tcgcgctcct ggacctggcg
      841 acggatctgg tcctcccagc gctcgccctc gcggagatcg agacagtcga cgaacacgtc
      901 tcccccgatc gcctcgatgg tcgagagccg ccccatcacc ttggcccggt cccggcgcgc
      961 atacgaggcg aaatagctgg tcggcatgcg ccggagcacc gtcgatacgg ccggtgcggt
     1021 ggtcgctgcc cggcgatcga tctcgagggc gatgtcgaac cgggcaagct ccacgccgcg
     1081 acgacccgcc gtcgagacag ccacggtcaa gatatgggtc ccgctcttga tccgctcgga
     1141 cgcgtgcagc ggaaacgaga cggacgaccg gggcggcctc cagacgacgc tgcgcgccgg
     1201 atccccggcg gtgatcccgg gaggtggccg gatcgcgatc tgcaagcggg ctccggcccg
     1261 cagccggacc acggcacgct ccgcggacac ggcggtgcgc gcggatgcgc gaggaccggc
     1321 gccgcggagg tgaagaaaga tctcgaccag gaacgaatcg ccgggccgga tggcgcgagg
     1381 gtgggttgcc gacagcatca catcctgcac acggtttgcc gccgtcgcct ttccgggtgt
     1441 cttcgtgggc atccacaagg cgggcggacg tcgcgaaggc attgccctcg cgtgcgcttt
     1501 cttcgcgtgc gctttcttcg caggcgcctt cttcgagggc cccatgcctc ccccgttcct
     1561 ggaccgatac cggcccgata cccgctctcg tgagcgtgca ttgccagtgt cactccgcac
     1621 ctttccggcg aagtctcccc gtattaccgg caagtcctgc ggggcacctc ggtcctccac
     1681 cttgacccgg gcgagccggt acggagacgt gctcagcacc cgatagggat gggagcatac
     1741 ctggcacgtg ccgatgtcgc ctgtggtcag ccccagcaca gcggggtcag gcttcacacc
     1801 atgaccgaac caagggcacc tcgtcatcgt cactccgtga agaccatcgt ctcacggatc
     1861 ggctctacca ggcgagcgcg acgtctcgcg tcgcgtcacg gccggcggcg cagccggagc
     1921 agcgccccgt ggtcgtggat cgccagctcc agcggccagc cgtcggcgtc ggtgacgagc
     1981 tccgaccgcg gtccgcgcgg cggcgtgatc tcgtagacct gcgtcacgcc tgccgcgccg
     2041 ccggagccgc cggctcgatc ctcggcgcac cggacctgga acgtccgctc gctgtcgtcg
     2101 atctcggacg ccagcccgac gtcgtgctcg atgagctcga tcgtctgccc gacgccgagt
     2161 ggtagcagcc gcggccgcca cagcacgatc cgggacagca ccgcatcggg cgcgagcagg
     2221 ccatcggccg cgatcgcgtg atcgagcgcg gccggcacgc cgggcagcga ggtcccacgg
     2281 gcgtggagct cgccgccgtc gcgcacgacg tcgaggtggc cgcggccggt cgccccgtcg
     2341 agatcgagcg cgagcg
//
LOCUS       DASVOR010000907         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_38354,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000907 DASVOR010000000
VERSION     DASVOR010000907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38354"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF516_17650"
     CDS             <1..203
                     /locus_tag="VF516_17650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8109563.1"
                     /translation="NGPELHRWHHAIDYAPPGKNYATKLSLWDHLFGTADRRPGKPAG
                     YGLADPFPDGFLAQQAHAFRRR"
     gene            complement(214..>3169)
                     /locus_tag="VF516_17655"
     CDS             complement(214..>3169)
                     /locus_tag="VF516_17655"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8109564.1"
                     /translation="ADDHVPASLRMRAKPSDGSAWSPMRWARRIAQVSPAGGPPGSIP
                     DPASGPDPAPPNGDPSAQGHPPAPTIPVTGTLIHREQVDSPSPVWVVDREMLASAGLP
                     NVGDVLQKLPVQGNAVNAQNDVGDGSTRINLRGLGTARTLVLLNGRRVVPSGLGANSS
                     VDLGTIPLAMIERVEVLKDGASAVYGSDAIAGVVNVITRTNMHGIEANAYNSTSNHAD
                     GTNYDLGLVAGYSWDRANITLSGGYQSQKPVMARDRDFSRQTYTYDYSCTADMQARGD
                     CIQTRLSGYSTSPSGRLNTMPSGGPPLNPVGCNTRYCTADGMGGFRNFIGSTATDFGD
                     TYNVETLSYLVTPSTRINLFSNGHYDITRNVHGFFEGQFNRRTSTQQLAEEPFSTRAT
                     GIVISADSFYNPFGQDVVDYDRRLVEVGPRTFHQEVSTTRLVVGLDGTIDEPLPGFKN
                     WKWEVSYDYGHSDSTQVTHGDLSLSLLANALGPSFLDTQGAHCGTSPANEIAGCVPLD
                     LLHPGSGHITPDAINYLAFTGMLSGFNEQHTALLQASGKLLDLPNHAGISLAIGGDAR
                     LERGGQQPDALTAIGDTTGTAFQPIRGSYKTTESFGELSVVPIANGETLKWFEIDAAG
                     RVYAYDTFGSGTTGKVSGLVRTLGGVAVRGSYGTAFRTPSISELFSAQVGSFPLLEDP
                     CDTKPPSKPVQTPLPPMVAAQCLATGVPANSTFGTSQQRVKLGGNPALQPEKGTVGTA
                     GIVYAPARGLDVTLDYWHIDIHDAITNLPAQTILSQCYQAGNDDFCNQIQRDPVTHGI
                     SQITALNQNVGGVTTSGLDFSAAYQYRNSLGMFRHALEGTYLFQYNVDTGTRDPSTMK
                     EQILQGRGVYDLGVFPDLKFNLFTTWVHPSGFGAGFNVRFIDGFQECEFDDCNDPRNL
                     RRDVSSYVTGDLFVTYRLKAGESTPSIAAGMNNVLNARPPAIYNGPVLNADESAYDFL
                     GRQFYLRLSQRF"
BASE COUNT          458 a         1026 c         1158 g          527 t
ORIGIN      
        1 tcaacggccc cgagctccac cgctggcacc acgcgatcga ctacgcgccg cccggcaaga
       61 actacgcgac caagctctcg ctgtgggacc acctgttcgg caccgccgac cgccgccccg
      121 gcaagcccgc cggctacggc ctcgccgacc cgttccccga cggcttcctc gcccagcagg
      181 cccacgcgtt ccgccggcga tagcgcgccg ggatcagaac cgctggctga gccggaggta
      241 gaactgccgg ccgaggaagt cataggccga ctcgtccgca ttcagcacgg ggccattgta
      301 gatcgccggc ggccgggcat tcaggacgtt gttcatgccc gccgcgatgc tcggcgtgct
      361 ctcgccggcc ttcaggcgat aggtgacgaa caggtcgccc gtgacgtagc tcgagacatc
      421 gcgccgcagg ttcctcgggt cgttgcagtc gtcaaactca cactcctgga aaccgtcgat
      481 gaaccggacg ttgaagccgg cgccgaatcc cgacgggtgg acccacgtcg tgaacaggtt
      541 gaacttcaga tccgggaaca cgccgaggtc gtagacgccg cggccctgga ggatctgctc
      601 cttcatcgtg gaagggtccc tggtgccggt gtcgacgttg tactggaaca ggtacgtgcc
      661 ctcgagcgcg tggcggaaca tgccgaggct gttccggtac tgatacgccg ccgagaaatc
      721 gaggcccgac gtggtcacgc caccgacgtt ctggttcagt gcggtgatct ggctgatgcc
      781 gtgcgtgacc gggtcgcgct ggatctggtt gcagaagtcg tcgttcccgg cctggtagca
      841 ctgcgacagg atggtctggg ccgggaggtt ggtgatcgcg tcatggatgt cgatgtgcca
      901 gtagtcgagg gtgacgtcga ggccgcgcgc cggcgcgtac acgatgcccg cggtgccgac
      961 ggtgcccttc tcgggctgca gggccgggtt accgccgagc ttgacgcgct gctgcgacgt
     1021 gccgaacgtc gagttggccg gcacgccggt cgcgaggcac tgcgccgcga ccatcggtgg
     1081 cagcggggtc tggaccggct tgctcggcgg cttggtgtcg cacgggtcct cgagcagcgg
     1141 gaagctgccg acctgggccg agaacagctc ggagatgctg ggcgtgcgaa acgcggtgcc
     1201 atagctgccg cggacggcga caccgcccag cgtgcggacc agaccgctga ccttgcccgt
     1261 ggtgcccgag ccgaacgtgt cgtacgcgta gacgcgcccc gcggcgtcga tctcgaacca
     1321 cttcagggtc tcaccgttcg cgatcgggac gaccgagagc tcaccgaagc tctcggtcgt
     1381 cttgtaggag ccccggatcg gctggaacgc ggtgccggtg gtgtcgccga tcgcggtcag
     1441 cgcgtcaggc tgctggccgc cgcgctcgag ccgggcatcg ccgccgatgg cgagcgagat
     1501 gccggcgtgg ttcggcaggt ccagcagctt gccgctcgcc tggagcagcg cggtgtgctg
     1561 ctcgttgaag cccgacagca tgccggtgaa cgccaggtag ttgatcgcat cgggcgtgat
     1621 gtggcccgag cccgggtgca gcaggtcgag cgggacgcag ccggcgatct cgttggccgg
     1681 cgacgtgccg cagtgcgcgc cctgggtatc caggaagctc ggccccagcg cgttggccag
     1741 gaggctgagg ctcaggtcgc cgtgggtcac ctgggtcgag tcgctgtggc cgtagtcgta
     1801 cgagacctcc cacttccagt tcttgaaccc cggcagcggc tcgtcgatcg taccgtcgag
     1861 gccgaccacg aggcgggtcg tgctgacctc ctggtggaag gtacgcgggc cgacctcgac
     1921 gaggcggcgg tcgtagtcga ccacgtcctg gccgaacggg ttgtagaagc tgtcggccga
     1981 gatcacgatg ccagtcgcgc gggtgctgaa cggctcctcg gcgagctgct gggtcgacgt
     2041 gcgtcggttg aactggccct cgaagaaccc gtggacgttc cgggtgatgt cgtagtggcc
     2101 gttcgagaac aggttgatcc gggtcgacgg cgtcaccagg tagctcaggg tctcgacgtt
     2161 gtaggtgtcg ccgaagtcgg tcgcggtgga gccgatgaag ttgcggaaac cgcccatgcc
     2221 gtccgcggtg cagtaccggg tgttgcagcc cacggggttc agcggcgggc cgccgctggg
     2281 catcgtgttg aggcggccgc tcggcgacgt cgaatagccg ctcagcctgg tctggatgca
     2341 atcgcccctg gcctgcatgt cggcggtgca gctgtagtcg tacgtgtacg tctgcctgga
     2401 gaagtcgcgg tcgcgggcca tcaccggctt ctggctctgg tagccgccgg agagcgtgat
     2461 gttggcgcgg tcccacgagt agccggccac gaggccgagg tcgtagttgg tgccgtcggc
     2521 gtggttcgat gtcgagttgt aggcgttggc ctcgatgccg tgcatgttgg tccgcgtgat
     2581 cacgttgacc acgccggcga tcgcgtccga tccgtagacc gccgatgcgc cgtccttgag
     2641 cacctcgacg cgctcgatca tcgccagcgg gatcgtcccg aggtcgaccg agctgttggc
     2701 gcccaggccg ctcggcacca cgcggcgccc gttgagcagc accagggtgc gggcggtgcc
     2761 gaggccgcgc agattgatgc gggtcgagcc gtcgccgacg tcgttctggg cattgaccgc
     2821 gttgccctgg acagggagct tctgcagcac gtcgccgacg ttggggaggc cggcgctggc
     2881 cagcatttcg cgatcgacca cccagaccgg cgacggcgag tcgacctgct cgcggtggat
     2941 cagcgtgccg gtgaccggga tcgtcggcgc cggcggatgg ccctgggcgg acggatcgcc
     3001 gttcggcggc gccggatcgg ggcccgacgc cggatcggga atcgaccccg gcgggccgcc
     3061 ggccggcgag acctgcgcga tccgccgggc ccagcgcatc ggcgaccacg ccgatccgtc
     3121 ggatggcttc gcgcgcatgc gcagcgaggc cggcacgtga tcgtcggcg
//
LOCUS       DASVOR010000908         1608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_476882,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000908 DASVOR010000000
VERSION     DASVOR010000908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1608
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476882"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF516_17660"
     CDS             <1..220
                     /locus_tag="VF516_17660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109565.1"
                     /translation="PATAPEALQRYEQIRRPRASQVQRMSRGREVQNHLDDGPAQEAR
                     DAALASSEPLRQSAWLYGHDVEADLARG"
     gene            complement(259..846)
                     /locus_tag="VF516_17665"
     CDS             complement(259..846)
                     /locus_tag="VF516_17665"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8109566.1"
                     /translation="MTKPSSRAPTLADLDALPPGIVGEIINGVLYTMTKPRMRHQRTT
                     TRIGSGVGDPFDMGRGGPGGWWIVVEPGIELPNTPEISPDVAGWRRERMPEMPGDEPI
                     RLVPDWVCEILSKTTRRHDQLVKMPYYAEVGVAYAWTVDLEARLLTAHRLESRHWVTV
                     GTYSNETEARIVPFDAVPLNVANWWPPAPSDGETP"
     gene            complement(948..>1608)
                     /locus_tag="VF516_17670"
     CDS             complement(948..>1608)
                     /locus_tag="VF516_17670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8109567.1"
                     /translation="ADNGTLFLDEVGDVPPAMQVKLLRALQEQEFERVGGEAPIKVDV
                     RVVSATNKNLEAEVAAGRFRQDLFFRLHVLPVKLPPLRERREDIPVLCQHFISKLAPK
                     TNTRVRAVSDAALGRIMAYHWPGNVRELENAIEQALVFAEGEEISPSALPQFLQGGAE
                     EDRLDVPRELSLPEILDDLERQLILKAYAKAGRVKTETARLLGIKTSALYYKLEKYSI
                     E"
BASE COUNT          223 a          559 c          576 g          250 t
ORIGIN      
        1 cccggccacg gcgcccgagg cgctgcagcg ctacgagcag atccgccggc cgcgcgcgag
       61 ccaggtccag cggatgagcc gcggccgcga ggtccagaac catctcgacg acggccccgc
      121 gcaagaggcc cgcgacgctg ccctcgccag cagcgagccg ctccgccaga gcgcctggct
      181 gtacggccac gacgtcgagg ccgacctggc gcgcggctag cgctcgcgcg gtggtaccgg
      241 tgacgatgcg gttgccagct acggcgtttc gccgtcgctg ggcgccggcg gccaccagtt
      301 ggccacgttg agcggaaccg cgtcgaacgg cacgatccgc gcctcggtct cgttgctgta
      361 cgtgccgacc gtgacccagt ggcgggattc caggcgatgc gcggtcagca ggcgcgcttc
      421 gagatcgacg gtccatgcgt aggcgacacc gacctcggcg tagtacggca tcttgacgag
      481 ctggtcgtgc cgccgcgtgg tcttcgagag gatctcgcag acccagtcgg gcaccagccg
      541 gatcggctcg tcgcctggca tctcgggcat gcgctcgcgg cgccagccgg ccacgtcggg
      601 cgagatctcg ggcgtgttgg gcagctcgat cccgggctcg acgacgatcc accagccgcc
      661 cgggccgccg cgtcccatgt caaacgggtc gccaacgccg gatccgatcc gggtcgtggt
      721 gcgctgatgc cgcatgcgcg gcttggtcat cgtgtacagc acaccgttga tgatctcgcc
      781 gacgatgccc ggcggcaggg catcgaggtc ggcgagcgtc ggggctctcg agctgggctt
      841 ggtcatgctc acgtccctac gctggcaggg tagcactgcc gggagaagcg cgcggagcct
      901 tcgtgtgaac gcggccgggc gattgccaca gcctcgttct ggaatcgcta ctcgatgctg
      961 tacttctcga gcttgtagta cagcgccgag gtcttgatgc cgagcaggcg ggcggtctcg
     1021 gtcttgaccc ggccggcctt ggcgtacgcc ttgaggatga gctggcgctc gaggtcgtcg
     1081 aggatctcgg gcagcgacag ctcgcgcggc acgtcgagcc ggtcctcctc ggcgccgccc
     1141 tgcaggaact gcggcagcgc ggacggcgag atctcctcgc cctcggcgaa caccagcgcc
     1201 tgctcgatcg cgttctccag ctcgcggacg ttgcccggcc agtggtaggc catgatccgg
     1261 ccgagcgcgg cgtcgctgac cgcgcggacc cgggtgttgg tcttgggcgc cagcttggag
     1321 atgaagtgct ggcacagcac cgggatgtcc tcgcggcgct cgcgcagcgg cggcagcttg
     1381 accggcagga cgtggagccg gaagaacagg tcctggcgga accgcccggc cgcgacctcg
     1441 gcctcgaggt tcttgttggt cgccgacacg acgcggacgt cgaccttgat cggcgcctcg
     1501 ccgccgaccc gctcgaactc ctgctcctgc agcgcgcgca gcagcttgac ctgcatcgcg
     1561 ggcggcacgt cgccgacctc gtcgaggaac agcgtgccgt tgtcggcg
//
LOCUS       DASVOR010000909         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_9603,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000909 DASVOR010000000
VERSION     DASVOR010000909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_9603"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1590)
                     /locus_tag="VF516_17675"
     CDS             complement(<1..1590)
                     /locus_tag="VF516_17675"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8109568.1"
                     /translation="MAVALGIDLGTTNSVAAVATPTGVEFVLGARGERVHPSVVAFPA
                     SGGVVAGTDAKRYRLSEPHHVVHSAKRFIGQNVRAPLVQLALTGVRYGVVEGPNQQPI
                     VVMRDRKMTMPEISSHVLLHLKRAAERQLGSQVQDAVITVPANFTDAQRQATREAGRL
                     AGLEVMRLINEPTAAALAYGFGQDREQMIVVFDFGGGTFDVSVLQLRGEVFEVLASEG
                     DFFLGGDDLDRALAEHLASELHRALRIDPRPHAALMMKLMMGAEAIKIHLSDNEAAEG
                     EIEGLDLPDGKVGTLPFALTRPQFEELIAGYVERTIAVTRQVLVAANIDPRMISDVLC
                     VGGSTRIPLVRRQLAEIFRREPNIAINPDEVVAQGAAIQAGSLTGALSAGTGMGARDA
                     VATVGHSPLAQGAVVLPETTARRPVLLDVNPAALAIHTAGGFTERLLDKNAPIPIERT
                     RVFTTSRDTQTRVEIACCRGEARRYAENEPLGTLLLDDLPPRPRGDVQIEVQFRVDAD
                     GILHVRASDRASGKAQEARLQV"
     gene            complement(1637..2092)
                     /locus_tag="VF516_17680"
     CDS             complement(1637..2092)
                     /locus_tag="VF516_17680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEX8109569.1"
                     /translation="MQRTVERMLRTHPEPAFAPAEALTTCIYACVECAQACTACADAC
                     IAEHDGALGPCIRISLDCADLCDAASRVLTRQLAPDLGVVSMTLQLCATVCDACAYEC
                     RRHAAAYPHCDVCAAACRRCTDACRGLLATTVTTYRNRAPYEAAEHVGA"
     gene            complement(2179..>2387)
                     /locus_tag="VF516_17685"
     CDS             complement(2179..>2387)
                     /locus_tag="VF516_17685"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8109570.1"
                     /translation="AIDVMEGHLGGHAWFTGECYGIADIALFAYTQSAAAIGFAVGPG
                     VGGWLDRVRAQPGHVPIKVDPTRA"
BASE COUNT          321 a          886 c          848 g          332 t
ORIGIN      
        1 cacctggagt cgcgcctcct gggccttccc cgacgcccgg tcgctcgcgc ggacgtggag
       61 gatgccgtcg gcgtcgaccc ggaactggac ctcgatctgg acgtcgccgc gcggccgcgg
      121 cggcaggtcg tcgagcagca gggtgccgag cggctcgttc tcggcgtagc gccgcgcctc
      181 accgcggcag caggcgatct cgacccgggt ctgggtgtcg cgcgaggtcg tgaacacccg
      241 cgtgcgctcg atcgggatcg gcgcgttctt gtcgagcagc cgctcggtga agccgccggc
      301 ggtgtggatc gccagcgccg ccgggttgac gtcgagcagc accggccgcc tggcggtggt
      361 ctcgggtagc accaccgctc cctgcgcgag cggcgagtgc ccgaccgtcg cgaccgcgtc
      421 gcgcgcgccc atcccggtgc cggccgacag cgcgccggtc aggctgccgg cctggatcgc
      481 ggcgccctgc gcgaccacct cgtcggggtt gatcgcgatg ttgggctcgc ggcggaagat
      541 ctcggcgagc tggcggcgca ccagcgggat ccgggtcgac ccgccgacgc atagcacgtc
      601 gctgatcatc cgcggatcga tgttcgccgc gaccagcacc tggcgggtca ccgcgatcgt
      661 gcgctcgacg tagccggcga tcagctcctc gaactgcggc cgggtcagcg cgaacggcag
      721 cgtgccgacc ttgccgtccg gcaggtcgag cccctcgatc tcgccctcgg ccgcctcgtt
      781 gtccgacagg tggatcttga tcgcctcggc gcccatcatc agcttcatca tcagcgcggc
      841 gtgcggccgc ggatcgatcc gcagcgcccg gtgcagctcg gacgcgaggt gctcggccag
      901 cgcgcggtcg aggtcgtcgc cgccgagaaa gaagtcgccc tccgacgcca ggacctcgaa
      961 cacctcgccg cggagctgca gcaccgacac gtcgaacgtg ccgccgccga agtcgaacac
     1021 cacgatcatc tgctcgcggt cctgtccgaa accgtaggcc agcgcggccg cggtcggctc
     1081 gttgatcagc cgcatcacct cgagcccggc gagccggccg gcctcgcggg tcgcctggcg
     1141 ctgcgcatcg gtgaagttgg cgggcaccgt gatcaccgcg tcctggacct ggctgccgag
     1201 ctggcgctcc gccgcgcgct tgaggtggag cagcacgtgc gacgagatct ccggcatcgt
     1261 catcttgcga tcgcgcatca ccacgatcgg ctgctggttc ggcccctcga cgacgccgta
     1321 gcgcaccccg gtcagcgcga gctggaccag cggcgcccgc acgttctggc cgatgaaccg
     1381 cttggccgag tgcaccacgt ggtgcggctc gctcagccgg tagcgcttgg cgtcggtccc
     1441 cgccaccacc ccgccgcttg cggggaacgc caccaccgac gggtggacgc gctcgccgcg
     1501 cgcgcccagg acgaactcga ccccggtcgg cgtcgcgacc gcggcgaccg agttcgtcgt
     1561 ccctagatcg atgccgagcg cgaccgccac tcgccccgag tgttacgcaa gaacggcggg
     1621 cgctgtcgag gaatcgtcac gccccgacgt gctcggccgc ctcgtacggc gcccggttcc
     1681 ggtaggtcgt gaccgtcgtc gcgagcagcc cgcggcacgc gtcggtgcag cgccggcacg
     1741 ccgccgcgca gacgtcgcag tggggatacg ccgcagcgtg ccgccggcac tcgtaggcgc
     1801 aagcatcgca caccgtcgcg cacagctgaa gcgtcatgct gaccacgccg aggtcgggcg
     1861 cgagctgccg ggtcagcacc cgggacgccg cgtcgcagag atccgcgcag tcgagcgaga
     1921 tccggataca cgggccgagc gcgccgtcgt gctccgcgat gcacgcatcg gcgcaggcgg
     1981 tgcatgcctg cgcgcattcg acacacgcgt agatgcacgt ggtcagggct tccgccggcg
     2041 cgaacgccgg ctcggggtgg gtacgaagca tccgctctac cgtgcgttgc atggggaaac
     2101 ctccggtgga tccggcgccc gtcgcaccgg atgcccggaa gctgtgcatg ccacgtgccc
     2161 cgcggcgtgg cgcgacggtc aggcgcgcgt ggggtcgacc ttgatcggga cgtgtccggg
     2221 ctgcgcgcgg acccggtcga gccagccgcc gacccccggc ccgaccgcga agccgatcgc
     2281 ggccgcgctc tgcgtgtacg cgaacagcgc gatgtcggcg atcccgtagc actcgccggt
     2341 gaaccaggcg tgcccgccga ggtggccctc catcacgtcg atcgccg
//
LOCUS       DASVOR010000910         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_155309,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000910 DASVOR010000000
VERSION     DASVOR010000910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155309"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1236
                     /locus_tag="VF516_17690"
     CDS             370..1236
                     /locus_tag="VF516_17690"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8109571.1"
                     /translation="MKISRGVLYMTTSAFGFSAMGVLVKLASARLPLGEIVFARAVIT
                     LGISLAMVRRAGLSPWAGPRMSLVFRGVLGFGGLTGYYIALDRLPLADATVLQNATPL
                     LTGVLAWWLLDEPIGWSTAIAIACGAGGVALIVRPSDDVIDPIGVIAALGGVACSSVA
                     YVTVRRLSRIVHPLVIVLYFPLIATPLALPWMLASFVVPGPVDLLLLVALAAATQVGQ
                     VFMTLGLSVESAGRATSINYLQVAFAMLWQLAVFGEFPTAWTVAGASLILGGTLLVAQ
                     VRGRTEPAPQTD"
     gene            1328..1888
                     /locus_tag="VF516_17695"
     CDS             1328..1888
                     /locus_tag="VF516_17695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109572.1"
                     /translation="MRLALISGLVATALAGLAAPAHADTLRRAVTPMARADGAVCDLL
                     RAELGDSCKRVAHDGNAAVYQSRGRSGGSGIRRLVLAIDTGSAVLVSPAVDVLDDQLE
                     SARPTLRPIAIDGRSGIALDVVSTWRRGNAAEHAESLIGCMQAGAIWKCSQVDVGSCD
                     ATLGSDGSITTSCGASSTLAVPAPGA"
     gene            1965..3890
                     /locus_tag="VF516_17700"
     CDS             1965..3890
                     /locus_tag="VF516_17700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8109573.1"
                     /translation="MMVTTRAEPPQLDAATLWRAIENAAEQAGMGVYIAHIDVQPPRI
                     LYVNPRAAEITGRSPEELIGRLPWSMLRDQDHPTVRAMIERPAGAPPASLALVIERPD
                     GKTVPIELAATRIRSGIGELSFGYFRDVSVEREAVAALQRSEARFRFLVESAPDGVVI
                     LKRGVIVYINPKAAQLLGLRGVDEAFGRPIAAFQPPEDAALAGKRIAAMLRDGVEFPP
                     SEYGVLGVPGRVVEIKSIASEWEGEQAVLAFARDVTERNAIQRRLVESDRLAALGTLS
                     VGVAHEINNPLTYAQLSAYQVARVLDTVGLPDQTMALLRGYLENIDHGIARVASIAQS
                     LRSFARDDADALGPVDIDVVLTHALKMVDNDLRHAARLVREVAPLPPVVGNAARLEQV
                     FVNVLINAIKALPPDPDRPHEIHVTADHVGDRVTVTIRDTGCGIPAALRGRIFEPFFT
                     TRDVGRGMGLGLSVSKTIVENHGGAIEVDSIEDAGTRVRVHLPAHRASAGAAAQAGVA
                     ADVAAPAAVVGSPRRRVLVVDDEALICRTIEQVLSESHDVAVATTGSDALALVASRKF
                     DCILCDVMMPDMNGHELYRQVAAQHPGLERRFVFMSGALAPSVAQVLEDLPNPWLAKP
                     FEIEDVLELIAASAAAG"
     gene            4000..>4232
                     /locus_tag="VF516_17705"
     CDS             4000..>4232
                     /locus_tag="VF516_17705"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8109574.1"
                     /translation="MIPSRATLRRLVRARDVLHASVQRGATLDELAAASGLSRGFLVR
                     SFARTFGAPPHQYLVQLRLEHAKRALARGASVT"
BASE COUNT          543 a         1502 c         1540 g          647 t
ORIGIN      
        1 ggcgggcgcg gcgcccccgg tcatcaacgt cgacaccagc gtgctcagcc gcgccggcgg
       61 actggccggc ctgctcggcg gcggctgggg cggcggtggc ggcggctgct gcggttgcgg
      121 ctgcaagtgc tggtagtccg cgcctcgcgg aggggctgac gtccgggacg accgcgtcgt
      181 cgggcgtgcg gccgtcggat cgcgagatct gtcgccgccg cgagcccgtc gtcggcgccg
      241 cccggcggat cgcatacgac gccatcgccc gccgggtccg gctcgggagc ctgtacagca
      301 caccgggatc gcgcacgaca cacgtcgccc aggcgccgtg cgcggccggt gcacgctaga
      361 gtgcgccgcg tgaagatctc gcgcggcgtg ctgtacatga cgaccagcgc gttcggcttc
      421 tcggcgatgg gggtcctggt caagctcgcg agcgcgcggc tgccgctcgg cgagatcgtg
      481 ttcgcgcggg cggtgatcac cctcgggatc tcgctcgcca tggtccgccg cgccgggctg
      541 tcgccctggg ccgggccgcg catgtcgctg gtgttccgcg gcgtgctcgg cttcggcggg
      601 ctcaccggct actacatcgc gctcgaccgg ctgccgctcg ccgacgcgac cgtgctgcag
      661 aacgcgacgc cgctgctcac cggcgtgctc gcgtggtggc tgctcgacga gccgatcggc
      721 tggtcgaccg ccatcgcgat cgcgtgcggc gccgggggcg tcgcgctgat cgtgcggccg
      781 agcgacgacg tgatcgatcc gatcggcgtg atcgccgcgc tcggcggcgt cgcctgctcg
      841 tcggtcgcgt acgtcacggt gcgccggctg tcgcgcatcg tgcacccgct ggtcatcgtg
      901 ctgtacttcc cgctgatcgc caccccgctc gcgctgccct ggatgctggc gtcgttcgtg
      961 gtgccggggc cggtcgacct gctgctgctc gtcgcgctcg ccgccgcgac ccaggtcggc
     1021 caggtgttca tgaccctggg cctgtcggtc gagagcgccg ggcgggcgac cagcatcaac
     1081 tacctgcagg tcgcgttcgc catgctgtgg cagctcgcgg tgttcggcga attcccgacg
     1141 gcctggaccg tggccggcgc gagcctgatc ctcggcggca cgctgctggt cgcgcaggtc
     1201 cgcggccgga cggagccggc gccccagacc gactgaccgc tgcgtcaagt atccgtgtaa
     1261 ttcgagttgg ataacgcgct gcgcggcgat tgaatgaaac cggcccggcg cgcgtttcac
     1321 tggcatcatg cgcctcgcgc tcatctccgg cctcgttgcc accgcgctcg ccggtctcgc
     1381 tgctccggcc cacgccgaca cgctgcgccg cgcggtgacc ccgatggcgc gcgccgacgg
     1441 cgcggtctgc gacctcctgc gcgccgagct cggcgacagc tgcaagcgcg tcgcccacga
     1501 cggcaacgcc gcggtctacc agtcgcgcgg caggtccggc ggctccggca tccgccgcct
     1561 cgtgctcgcg atcgacaccg gcagcgcggt cctcgtcagc ccggcggtcg acgtgctcga
     1621 cgaccagctc gagtccgccc ggccgacctt gcgcccgatc gccatcgacg gccgctccgg
     1681 catcgcgctc gacgtcgtgt cgacctggcg gcgcggcaac gccgccgagc acgccgagtc
     1741 gctgatcggc tgcatgcagg ccggcgcgat ctggaagtgc tcgcaggtcg acgtcggcag
     1801 ctgcgacgcg accctcggca gcgacggcag catcaccacc tcgtgcggcg ccagctcgac
     1861 cctcgcggtc ccggcgccgg gcgcctagcc gccgcgcgtc actcgcgatc ggcctgggcc
     1921 gggcccggat acccgtggat cggctcgtcc gaggcgtgct atgtatgatg gttacgaccc
     1981 gcgccgagcc accgcagctc gatgccgcca cgctatggcg cgcgatcgag aatgccgccg
     2041 agcaggcggg catgggcgtg tacatcgcgc atatcgatgt ccagccacct cgcattctct
     2101 atgtcaatcc acgcgcggcc gagatcaccg gccgctcgcc cgaggagctg atcggccgtt
     2161 tgccgtggag catgctgcgc gaccaggacc atcccacggt gcgcgcgatg atcgagcggc
     2221 cggccggagc gccgccggcg tcgctggcgc tggtcatcga gcggccggat gggaagaccg
     2281 tgccgatcga gctggcggcg acccggatcc ggagcgggat cggcgagctc agcttcgggt
     2341 acttccgcga tgtcagcgtc gagcgcgagg cggtcgcggc gctgcagcga tcggaagcgc
     2401 ggttccggtt tctcgtcgaa tccgcgccgg acggcgtcgt gatcttgaag cgcggagtga
     2461 tcgtgtacat caacccgaag gcggcgcagc tgctcggtct ccgcggtgtc gacgaggcgt
     2521 tcggccggcc gatcgcggca ttccagccgc cggaggacgc ggcgctggcg ggcaagcgca
     2581 tcgccgccat gctgagagac ggtgtggagt ttccgccgag cgagtacgga gtgctcggcg
     2641 ttccgggccg cgtcgtggag atcaagtcga tcgcgtccga gtgggagggc gagcaagccg
     2701 tgctggcgtt cgcgcgcgac gtcaccgagc gcaatgcgat ccagcgccgg ctcgtcgagt
     2761 cggaccggct ggccgcgctg ggaacgctgt cggtgggcgt cgcgcacgag atcaacaatc
     2821 cgctgacgta cgcccagctg agcgcttacc aggtggcgcg agtgctcgac accgtcgggc
     2881 tgccggacca gaccatggcg ttgctgcgcg gatacctcga gaacatcgat cacggcatcg
     2941 cgcgggtcgc gtcgatcgcg cagtcgctcc ggtcgttcgc cagggacgat gccgacgcgc
     3001 tgggaccggt ggacatcgac gtcgtgctca cgcacgcgct caagatggtg gacaacgacc
     3061 tgcggcacgc ggctcggctc gtgcgcgagg ttgcgccgct gccgcccgtc gtcgggaatg
     3121 cggcgcgact cgaacaggtg ttcgtgaacg tgctgatcaa cgcgatcaag gcgctgccgc
     3181 ccgatccgga ccggccgcac gagatccacg tgacggcgga ccatgtcggc gatcgcgtca
     3241 cggtcacgat ccgagacacc ggctgcggga tccccgcagc gctgcggggc cggatcttcg
     3301 agccgttctt cacgacgcga gacgtcgggc gcgggatggg cctggggctc tcggtgtcga
     3361 agacgatcgt cgagaaccac ggcggggcga tcgaggtgga ctcgatcgaa gacgccggca
     3421 cgagggtgcg cgtccatctc ccggcgcatc gggcgagtgc cggagcggcc gcgcaggcgg
     3481 gcgtggcggc cgatgtcgcg gcacctgccg cggtggtcgg ctcgccgcgc cgtcgcgtgc
     3541 tcgtcgtcga cgacgaggcg ctgatctgcc gtaccatcga gcaggtactg tcggagagtc
     3601 acgacgtcgc cgtggcgacg accggaagcg atgcgctcgc gctggtcgcg tcgaggaagt
     3661 tcgactgcat cctgtgcgac gtgatgatgc cggacatgaa cggtcacgag ctgtatcgcc
     3721 aggtcgccgc gcagcatccc gggctcgagc gccggttcgt cttcatgagc ggcgccctcg
     3781 caccgagcgt cgcgcaggtg ctcgaggatc tgcccaaccc gtggctcgcc aagccgttcg
     3841 agatcgagga tgtgctcgag ctgatcgccg cgtcggcggc cgcgggctga tgcccgtcac
     3901 tcgaccgcga agtccgccgc ggtcggcgga tcgcactcga agatcggtgc cgtgatccgg
     3961 tacgcatggc cgtccccgtg agtctggtat gacttccgga tgatcccatc gcgagcgacg
     4021 ctgcgccggc tggtccgcgc ccgcgacgtc ctccacgcca gcgtgcagcg cggcgcgacc
     4081 ctcgacgagc tcgccgcggc ctcggggctg tcgcgcggct tcctggtccg ctcgttcgcc
     4141 cggacgttcg gcgcgccgcc gcaccagtac ctggtccagc tccgcctcga gcacgccaag
     4201 cgcgcgctcg cccgcggcgc cagcgtgacc ga
//
LOCUS       DASVOR010000911         2022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_388792,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000911 DASVOR010000000
VERSION     DASVOR010000911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388792"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /locus_tag="VF516_17710"
     CDS             complement(<1..767)
                     /locus_tag="VF516_17710"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX8109575.1"
                     /translation="MSEPLRLMAVMAHPDDESLGFGGTLAKYIAEGVETSLVTATRGE
                     AGRYRGIAPGAEGHPGATELARIREVELREAAAVLGIRDLTLLDYRDQELDRADPREA
                     IGLIATHLRRVRPQVVVTFAHDGAYGHPDHVAICQFTTAAVVAAADAGYASPALDRTH
                     APHAVSKLYYIAWPAAAMAAYQEAFRKLIARVDGVDRESTPWPDWALSAVIDTHAHWE
                     QVWRAVSCYQSQITAYEKLHHLSPEGHEGLWGWQSFYR"
     gene            complement(840..>2022)
                     /locus_tag="VF516_17715"
     CDS             complement(840..>2022)
                     /locus_tag="VF516_17715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109576.1"
                     /translation="AVNIKTGERRNLVKGARRAIWAPPGYLVFDRAGDLYAMRIDPRD
                     PRAGSEPQPVLGHVEGETSSGAGFFDISNDGTLAWVRAEGGSDQREIGWMDREGRWTP
                     TKFPPGPYFDVQVSPEGSRVLVSAGPGGGASDLWVGDLASGAMNRLTYGSTANPGRWL
                     RDGTSMVYAKGDSAGGTSIVVRRLDGAGGERVLDHKPWPMLVTGLTPDGARVLYCDYG
                     LAQGRLRIANLAGAPEARELRADPGNERNEQAGSVSPDGRWLAYVSNRTRREEVYVCG
                     FDGAGGRWQVSTRGGGGVRWGRDSRELFFVEQGSERLMRVSLQVAGTELVVGQPEPLF
                     TASPSPTEPTYRDFDYDRAHDRFLFTRPPAGVNERREIALSLGWTSRLDAQMRTRKSA
                     N"
BASE COUNT          274 a          731 c          729 g          288 t
ORIGIN      
        1 cggtagaacg actgccagcc ccacagccct tcgtggccct cgggcgagag gtgatgcagc
       61 ttctcgtagg cggtgatctg cgactggtag caggacaccg cgcgccacac ctgttcccag
      121 tgcgcgtgcg tgtcgatgac cgcgctcagc gcccagtcgg gccacggcgt gctctcgcgg
      181 tcgacgccgt cgacgcgcgc gatcagcttg cggaacgcct cctggtaggc ggccatggcc
      241 gcggcgggcc acgcgatgta atagagcttc gagaccgcgt gcggcgcgtg agtacggtcg
      301 agcgcgggcg aggcgtagcc ggcatccgcg gccgcgacca cggccgcggt cgtgaactgg
      361 cagatcgcga cgtggtcggg gtgcccgtag gcgccgtcgt gcgcgaacgt caccacgact
      421 tgcggccgca cgcgacgcag gtgggtcgcg atcagcccga tcgcctcgcg cgggtcggcg
      481 cggtccagct cctggtcgcg gtagtcgagc aacgtcaggt cgcggatgcc cagcacggcg
      541 gctgcctcgc gcagctccac ctcgcggatc cgggcgagtt ccgtggcacc gggatgcccc
      601 tcggcgccgg gcgcgatgcc gcggtagcgc ccggcctcac cacgtgtcgc ggtgacgagc
      661 gacgtctcga cgccctcggc gatgtacttg gccagcgtgc cgccgaagcc gagcgactcg
      721 tcgtcggggt gtgccatcac ggccatgagg cggagtggtt cggacatgcg tgcgacctcc
      781 agagggggtg gcgacacggt gcagtcgcga ggaaccgagt gcaagcgcgg ccccagccgt
      841 cagttggcgc tcttccgggt gcgcatctgc gcgtcgaggc gcgacgtcca gccgagcgac
      901 agcgcgatct cgcggcgttc gttcacgccc gccggcgggc gcgtgaacag gaagcggtcg
      961 tgagcgcggt cgtaatcgaa gtcgcggtac gtgggctcgg tcggcgaggg gctcgccgtg
     1021 aacagcggct cgggctgccc gaccaccagc tccgtgccgg ccacctggag cgagacccgc
     1081 atcagccgtt cgctgccctg ctcgacgaag aacaactcgc ggctgtcgcg tccccagcgc
     1141 acgccaccgc caccgcgggt ggagacctgc cagcgtccgc ccgctccgtc gaagccgcag
     1201 acgtagacct cctcgcggcg cgtgcggttg gacacgtagg ccagccagcg gccgtcgggc
     1261 gagaccgacc ccgcttgctc gttgcgctcg ttgccggggt cggcgcgcag ttcgcgtgct
     1321 tccggcgccc ccgcgaggtt cgcgatccgc agccgcccct gcgcgagccc gtagtcgcaa
     1381 tagagcaccc gggcgccgtc cggcgtgagc ccggtgacga gcatcggcca gggcttgtgg
     1441 tcgagcacgc gctcgccgcc ggcgccgtcc agccggcgca cgacgatgct cgtcccgccg
     1501 gcggaatcgc ccttcgcgta caccatgctc gtgccgtcac gcagccagcg gccggggttc
     1561 gcggtgctgc cgtaagtgag ccggttcatc gcgccgctcg cgagatcgcc gacccacagg
     1621 tccgaggctc cgccgccggg ccccgcgctg accagcacgc ggctgccctc cggcgacacc
     1681 tggacatcga agtacggtcc gggcgggaac ttcgtgggcg tccagcggcc ctcgcgatcc
     1741 atccagccga tctcacgctg gtcgctcccg ccctcggcgc gcacccatgc gagcgtgcca
     1801 tcgttcgaga tgtcgaagaa cccggcgccg ctcgaagtct cgccctcgac gtggccgagg
     1861 acgggctgcg gctcggagcc ggcgcgcggg tcacgcggat cgatgcgcat ggcgtagaga
     1921 tcgccggcgc ggtcgaacac gagatagccc ggaggcgccc agatcgcccg ccgtgccccc
     1981 ttcaccaggt tgcggcgttc gccggtcttg atgttgacag cg
//
LOCUS       DASVOR010000912         1963 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_535234,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000912 DASVOR010000000
VERSION     DASVOR010000912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1963
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535234"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VF516_17720"
     CDS             <1..418
                     /locus_tag="VF516_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109577.1"
                     /translation="HGRLGALHGLALVAWIAVALGGARAASARGDHTTCNYTPAFAEA
                     LRQAHPEADLDGDGVLSRDEACAFQAELRHRVEQPPGADLVSRADIPALDALDETLLT
                     QPLCCNCDRGAEDSAPGAPWIEQRSDTCPSDEGVSR"
     gene            415..1272
                     /locus_tag="VF516_17725"
     CDS             415..1272
                     /locus_tag="VF516_17725"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8109578.1"
                     /translation="MNKLIVFCVVMVAACQRDDSGLSHKIDELNKRLDRLEPMLARGG
                     VGAAPQQRPARAEPDRAKTYAVPVDGDPSEGPADAKVTVVKAYDYASPFCEKVRDSME
                     ELRKKYPNDVRIVYKQFVVHPQVATAGALAVCAADKQGKFKELDALLWDKGFKGRQFD
                     KDATAEAGGSQKCWDTAAGCPVVLGYAQELGLNLDKFKADMKGECQQLVQKDMRELTA
                     LGVGSTPTFFINGRYMVGAAPTDSFVTLVDEELKKANERIAAGTPAASYYQQAVLDKG
                     LKQLERPQQ"
     gene            1317..>1963
                     /locus_tag="VF516_17730"
     CDS             1317..>1963
                     /locus_tag="VF516_17730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109579.1"
                     /translation="MALLAAIPDRNWVNIQWLARLRWAEIAGQAATVLVGQFLLDGAL
                     PMAELLAVIAVGLVSNLVIELYYFGDRRRPGAVARPVHERQIALVMMLDIAVLTGLLY
                     FSGGPHNPFAFLYVVQIALATVLVRALWSWMLVGLSFVGFGILLIAHQPLLIPPNRQM
                     IGAWMALGVASAFVVHFLRRITGALAERDAELTLARGLAARQERLASLATMAAGAA"
BASE COUNT          277 a          693 c          706 g          287 t
ORIGIN      
        1 gcatggccgg ctcggcgcgc tgcacggcct cgcgctggtc gcgtggatcg cggtcgcgct
       61 cggcggggcc cgggccgcct cggcgcgcgg cgatcacacc acctgcaact acacacccgc
      121 gttcgccgag gcgctgcgcc aggcccaccc cgaggccgac ctcgacggcg acggcgtgct
      181 gtcgcgcgac gaggcctgtg cgttccaggc cgagctgcgc caccgcgtcg agcagccgcc
      241 gggggccgac ctggtgtcgc gggccgacat acccgcgctc gacgcgctcg atgagaccct
      301 gctgacccag cccttgtgtt gcaattgcga ccgcggtgcg gaagactccg cgcccggggc
      361 gccctggatc gaacagcgct cggacacgtg cccgtccgac gaaggagtga gtcgatgaac
      421 aagctgatcg tgttttgcgt cgtcatggtg gccgcgtgcc agcgcgatga cagtggcctg
      481 tcgcacaaga tcgacgagct gaacaagcgg ctcgaccggc tcgagccgat gctcgcccgc
      541 ggcggcgtcg gtgcggcgcc gcagcagcgg ccggcccgcg ccgagcccga tcgcgccaag
      601 acctacgcgg tgccggtcga cggcgatccg tccgagggcc cggccgacgc caaggtcacc
      661 gtggtcaagg cctacgacta cgcgtccccg ttctgcgaga aggtccgcga ctccatggag
      721 gagctgcgca agaagtaccc gaacgacgtg cgcatcgtct acaagcagtt cgtcgtccac
      781 ccgcaggtcg ccaccgccgg cgcgctggcg gtgtgcgcgg ccgacaagca gggcaagttc
      841 aaggagctcg acgcgctctt gtgggacaag ggcttcaagg gccgccagtt cgacaaggac
      901 gccaccgccg aggccggcgg gtcgcagaag tgctgggaca ccgccgcggg gtgcccggtc
      961 gtgctcggct acgcgcagga gctcggcctc aacctcgaca agttcaaggc cgacatgaag
     1021 ggcgagtgcc agcagctggt ccagaaggac atgcgcgagc tcaccgcgct cggggtcggc
     1081 tcgacgccga cgttcttcat caacggccgc tacatggtcg gcgcggcgcc gaccgactcg
     1141 ttcgtcacgc tggtcgacga ggagctcaag aaggccaacg agcggatcgc cgccggcacg
     1201 ccggccgcga gctactacca gcaggcggtg ctcgacaagg gcctcaagca gctcgagcgg
     1261 ccgcagcagt agctttccgc ggcggtccgc cggtgatctg ctaggctggc gcgctgatgg
     1321 cgctgctcgc ggcgatcccg gaccgcaact gggtcaacat ccagtggctc gcgcggctgc
     1381 gctgggccga gatcgccggg caggcggcga ccgtgctggt cggccagttc ctgctcgacg
     1441 gggcgttgcc gatggccgag ctgctcgcgg tgatcgcggt cggcctggtg tccaacctcg
     1501 tgatcgagct gtactacttc ggggaccgcc ggcggcccgg cgcggtcgcc cggccggtcc
     1561 acgagcgcca gatcgcgctc gtgatgatgc tcgacatcgc cgtgctcacc ggccttctgt
     1621 acttcagcgg cgggccgcac aacccgttcg cgttcctcta cgtggtgcag atcgcgctgg
     1681 cgaccgtgct ggtccgggcg ctgtggagct ggatgctggt cgggctgtcg ttcgtcgggt
     1741 tcggcatcct cttgatcgcg caccagccgc tgctcatccc ccccaaccgg cagatgatcg
     1801 gggcgtggat ggcgctcggc gtggcctcgg cgttcgtcgt ccacttcctg cgccggatca
     1861 ccggcgcgct ggccgagcgc gacgccgagc tgaccctcgc gcgcgggctg gcggcgcgcc
     1921 aggagcgcct ggcgtcgctc gcgacgatgg cggccggcgc ggc
//
LOCUS       DASVOR010000913         2311 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_242948,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000913 DASVOR010000000
VERSION     DASVOR010000913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242948"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VF516_17735"
     CDS             complement(<1..602)
                     /locus_tag="VF516_17735"
                     /EC_number="3.8.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HEX8109580.1"
                     /translation="MAILRTPDERFTGLPGYPFEPHYVDVLGLRMHCVDLGSGAPVLL
                     LHGEPSWSYLYRKMIPPLAAAGLRAVAPDLIGFGRSDKPDARDDYTYQRHMDWLTAFV
                     VALDLRDIVLVCQDWGGLLGLRLAAEQPERFARIVAANTYLPTGDRRPSDAFFAWQQF
                     SQSVAELPVGNIVKSGCHVAPPPEVIAAYDAPFPDERYKAG"
     gene            complement(653..1726)
                     /locus_tag="VF516_17740"
     CDS             complement(653..1726)
                     /locus_tag="VF516_17740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8109581.1"
                     /translation="MLALEGNRPAPGKRERLVDILDRAGVLRRVMQLRRLSPLPIVGI
                     FTFHHIHDAAGSDAYPYDPEVADATPEQFRRHLETLLRIGTPITMAELLRGLGGAALP
                     PNPLMITFDDGYRSCHDVALPILQRLGIPATFFIATSFVAERRLYWWERIALTLHAAR
                     RPRAMITYPRPIEIDARDPGTRRMLDELIKRTWSLDVERFLDELCRALDVEWSPAIEA
                     EHADRLIMTRAQVRALADAGMSVQSHTRNHRVLDTLDDAALRDELLGARLDLEAEIGR
                     PVEAIAYPVGRPVQQPRIRAAIATAGYRVGFANAGGVNPLWPSVLRGAMAFDPFDLRR
                     IATGSSMSEAMFLTQLAIPPLGY"
BASE COUNT          355 a          822 c          825 g          309 t
ORIGIN      
        1 ccggccttgt agcgctcgtc ggggaacggc gcgtcgtagg cggcgatcac ctcgggcggc
       61 ggggcgacgt ggcagccgga cttgacgatg ttgccgaccg gcagctcggc cacgctctgg
      121 gagaactgct gccacgcgaa gaacgcgtcg gacggcctgc ggtcgccggt cggcaggtac
      181 gtgttggcgg cgacgatccg cgcgaaccgc tcgggctgct cggcggcgag ccggagaccg
      241 agcagcccgc cccagtcctg gcacaccagg acgatgtcgc gcagatcgag cgcgacgacg
      301 aacgcggtca accagtccat gtggcgctgg taggtgtagt cgtcgcgcgc gtcgggcttg
      361 tcggaccggc caaagccgat cagatcgggc gcgaccgcgc gaagcccggc cgccgccagc
      421 ggcgggatca tcttgcggta gaggtagctc cacgacggct cgccgtgcag cagcagcacg
      481 ggcgcgccgc tgcccaggtc gacgcagtgc atgcggaggc cgagcacgtc gacgtagtgc
      541 ggctcgaacg ggtagcccgg caggccggtg aaccgctcgt ccggggtgcg caggatcgcc
      601 atgcgcacga cgctaccaga ccgcggcgcg ccccgatcag gtcgagtcca ggtcagtaac
      661 cgagcggcgg gatcgcgagc tgggtcagga acatcgcctc ggacatcgag ctcccggtcg
      721 cgatccgccg caggtcgaac ggatcgaacg ccatcgcgcc gcgcagcacc gacggccaca
      781 gcgggttgac gccgccggcg ttggcgaacc cgacccggta gcccgcggtg gcgatcgcgg
      841 cgcggatccg cggctgctgg accgggcggc cgaccgggta cgcgatcgcc tcgaccggcc
      901 ggccgatctc ggcctcgaga tcgagccggg cgccgagcag ctcgtcgcgc agcgcggcgt
      961 cgtcgagcgt gtcgagcacg cggtggttcc gggtgtggga ctggaccgac atgccggcgt
     1021 ctgcgagcgc gcggacctgg gcgcgggtca tgatcagccg gtcggcgtgc tcggcctcga
     1081 tcgccgggct ccactcgacg tccagcgcgc ggcacagctc gtcgaggaag cgctcgacgt
     1141 cgagcgacca ggtccgcttg atcagctcgt cgagcatgcg tcgcgtcccc ggatcgcgcg
     1201 cgtcgatctc gatcggccgc gggtacgtga tcatcgcgcg cggccgccgg gcggcgtgca
     1261 gggtcagcgc gatgcgctcc caccagtaca gccggcgctc ggcgacgaac gaggtcgcga
     1321 tgaagaacgt cgccgggatc ccgagccgct gcaggatcgg cagcgcgacg tcgtggcagc
     1381 tccggtagcc gtcgtcgaac gtgatcatca gcgggttggg cggcagcgcg gcgccgccca
     1441 gcccgcgcag cagctcggcc atcgtgatcg gtgtgccgat ccgcagcagg gtctccaggt
     1501 ggcggcggaa ctgctccgga gtcgcatcgg cgacctcggg atcgtacgga tacgcgtcgc
     1561 tgcccgccgc gtcgtggatg tggtgaaatg taaatatccc gacgatcggc agcggcgaca
     1621 gccgccgcag ctgcatcact ctacgcagca cgcccgcgcg atccaggata tcgaccaacc
     1681 gctcacgctt gcccggcgcc ggccggttgc cctcgagtgc cagcacagat cctcctgcca
     1741 tccaccgccg agcaatggac atgcccaccg cctcctcgtt ccagcatagc gatccagccg
     1801 agggtgtcca gccggctcag agcatcgacg acctcaccgg aaagtcttgc tttcaccccg
     1861 cgatcgcgcg gcgcgccccg cgaactgctc agaatgcgca gggcccgatc gacgcgacgc
     1921 tcacgcccgc ccgctccgcg tcgcagccgg tcgggtattg cttgccatcg catccgcaca
     1981 cgatcacatc ggtgcatggc ccaggcgtcg tgaggatcgg tgcgcatgcg ccggggccgg
     2041 cgcacatgcc gagcggcgtg ttgcagaact ggccggatgg acacggcatc tcgcacgcgg
     2101 acagcgcgtc ggaggcggtg ccaccgtcgc ctgggcgccc tccgggtccg cccgccaccg
     2161 gtgcgccggc atcaccgggt ccatggccct ccgcgtcgaa gccgatccgc ccacagccgg
     2221 ccaccagcag cgcgatgcac ggcagggttc cccagcgcac accggatcat agatcgtccg
     2281 agccggcgat cgcgaacggc aatttctggc g
//
LOCUS       DASVOR010000914         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_155349,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000914 DASVOR010000000
VERSION     DASVOR010000914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155349"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1568
                     /locus_tag="VF516_17745"
     CDS             <1..1568
                     /locus_tag="VF516_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109582.1"
                     /translation="PPPQGCLASSECPTGWTCNDFHVCMPPAPGSDGGVPPETELEAG
                     PPIGAQRFVYVAMTAQGKLARIDGNTLAVSTTPVGPAPREVATIPGSDGAIALDAVNG
                     TATVVRPVVTGGDGTRVLATLPGLNRLDLDPTGRFAVIWFDVVRARQDGTPTSGSFQD
                     AVIASLSPGHESSVALTVGFRPRAVQFDAAGAHAFVITEDGVSVIDLADAIGHGPRIV
                     PPIAVAAPGVSPDDLEVQVVATGDYAAVRQAGHAGVRVVGLASATAGQVWDIPLAAPA
                     TDIDLAPDGSRLYAVVRDTAQLAVIDIPADAIDPSGVDLVDLAPATVGSIAVSADGTR
                     ALLYTNATLDPRLTAVALDQPGFPHATYALEKAVRAVAIAPDSATAVVLSAKAPGDPA
                     TASSLDDLIARSFGFELVDLASGFAKLQLTPVDPGAPAFAPAGDTAYVALDGGDAVAA
                     IRAVQIVSTRTGIVRTVALGSPPSSVGVVPLAGQAFVAQRHPLGRMSFVALATGAVRT
                     VTGFDLNSQIVDH"
     gene            1571..>1857
                     /locus_tag="VF516_17750"
     CDS             1571..>1857
                     /locus_tag="VF516_17750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109583.1"
                     /translation="MRTHPARSLAALAALGVAGALAAPACWSDDRPVRWDRPRDLLGP
                     IALKAQVAYIDPALDRVVLVDLTGDHPAVAHTAIGRRAMYAVPSDDRHRLFV"
BASE COUNT          215 a          755 c          642 g          245 t
ORIGIN      
        1 agcccccgcc acaggggtgc ctggcgtcga gcgaatgccc gacgggttgg acgtgcaacg
       61 actttcacgt ctgcatgccg ccggcgccgg gctccgacgg tggcgtgccg cccgagaccg
      121 agctcgaggc cgggccgccg atcggcgccc agcgcttcgt ctacgtcgcg atgaccgcgc
      181 agggcaagct ggcgcggatc gacggcaaca ccctcgcggt ctcgaccacg ccggtcggcc
      241 ccgcgccccg cgaggtcgcg accatcccgg gcagcgacgg cgcgatcgcg ctcgacgcgg
      301 tcaacggcac cgcgaccgtg gtccgcccgg tcgtgaccgg cggcgacggc acgcgcgtgc
      361 tggcgacgct gcccggcctc aaccggctcg acctcgaccc gaccggccgg ttcgcggtga
      421 tctggttcga cgtcgtccgc gcccgccagg acggcacccc gacctcgggc agcttccagg
      481 acgccgtgat cgcatcgctg tctcccggcc acgagagctc ggtcgcgctc accgtcgggt
      541 tccggccgcg cgccgtgcag ttcgacgccg ccggcgcgca cgcgttcgtg atcaccgagg
      601 acggcgtgtc ggtgatcgac ctcgccgacg cgatcggcca cgggccgcgg atcgtgccgc
      661 cgatcgcggt cgccgcgccg ggcgtatcgc ccgacgatct cgaggtccag gtcgtcgcga
      721 ccggcgacta cgcggcggtc cgccaggccg ggcatgccgg cgtacgggtg gtcggcctcg
      781 cgagcgccac cgccggtcag gtatgggaca tcccgctcgc cgcgccggcg accgacatcg
      841 acctcgcgcc cgacggctcg cggctctacg cggtggtgcg cgacaccgcg cagctggcgg
      901 tgatcgacat cccggccgac gcgatcgacc cgtcgggcgt cgacctcgtc gacctcgcgc
      961 cggccaccgt cggctcgatc gcggtgtcgg ccgacggcac gcgcgcgctg ctctacacca
     1021 acgcgacgct cgatccccgc ctgaccgcgg tcgcgctcga ccagccgggc ttcccgcacg
     1081 cgacctacgc gctggagaag gcggtgcgcg ccgtcgcgat cgcccccgac agcgccaccg
     1141 cggtcgtgct gtccgccaag gcgcccggcg atcccgcgac cgcgagctcg ctcgacgacc
     1201 tgatcgcgcg cagcttcggc ttcgagctgg tcgacctcgc gagcgggttc gccaagctcc
     1261 agctgacgcc ggtcgatccc ggcgcaccgg cgttcgcccc ggccggcgac accgcgtacg
     1321 tcgcgctcga cggcggcgac gcggtggccg cgatccgcgc ggtgcagatc gtgtcgaccc
     1381 ggaccggcat cgtccgcacc gtcgcgctcg gctcgccgcc gtcgtcggtc ggcgtcgtcc
     1441 cgctcgccgg ccaggcgttc gtcgcccagc gccacccgct cggccggatg tcgttcgtcg
     1501 cgctcgccac cggcgcggtt cgcaccgtca ccggcttcga cctcaacagc cagatcgtgg
     1561 atcactgacc atgcgcaccc accccgctcg ctcgctcgct gcgctcgcag ctctcggcgt
     1621 cgccggcgcg ctcgccgccc cggcgtgctg gagcgatgac cgcccggttc gctgggaccg
     1681 cccgcgcgat ctgctcggcc cgatcgcgct caaggcccag gtcgcgtaca tcgacccggc
     1741 gctcgaccgc gtcgtcctgg tcgacctcac cggcgaccac ccggcggtcg cccacaccgc
     1801 gatcggccgg cgcgcgatgt acgcggtgcc gagcgacgac cggcaccggc tgttcgt
//
LOCUS       DASVOR010000915         2088 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_505832,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000915 DASVOR010000000
VERSION     DASVOR010000915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505832"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..584)
                     /locus_tag="VF516_17755"
     CDS             complement(<1..584)
                     /locus_tag="VF516_17755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8109584.1"
                     /translation="MVNILVAEKSRAARQAIVEALGRIDTIAVQGAVAELREAVRSLC
                     RDTPDVVVTGVELADASGLDLIEAARQLSPTSRIVVVGPSPASEVWRRQVTAGAIRFV
                     AAGPSLDELCEVVAALARSAPEQATGAGDADDDPLRLLGRITAGVAHDLGNTLSALAA
                     VVALVDSAGADPALVARAAELVDHAGRITRSLIG"
     gene            complement(711..1334)
                     /locus_tag="VF516_17760"
     CDS             complement(711..1334)
                     /locus_tag="VF516_17760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8109585.1"
                     /translation="MYRVLLVDDHEVVRKGLRAILDDRFAGIEVAEAGGGDEALAALS
                     RPFDAVILDLSMPGRSGLDLLSEIKHRYPRLPVLIMSLHGEEQFAVRALRAGAAGYLT
                     KSAAPEQLITAFERIVRGGRYITEAVAERLAASAGGEQGAPHDRLSQREFEVMRGIAS
                     GETVGEIAERMHLSAKTVSTYRARLLDKMGMASNAELTRYALQNGLV"
     gene            1363..>2088
                     /locus_tag="VF516_17765"
     CDS             1363..>2088
                     /locus_tag="VF516_17765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8109586.1"
                     /translation="MADGVERGPSPGSSPGDPPGRTPGYAPGRTIERELRRQLDTTQS
                     ITHIGSWEWKMGGGAVTWSDELYRIYGLEPRSIPVTIELFLSRIHRDDRDRIEREIQS
                     ALVHPGRFAYRERIVRPDGSVRTLDTIGEAIADERGTTTRLVGTCLDVTEAVAREARI
                     RFYADVFEYAEIGLSAFQLDQPPGHTPDLPPGPPVLRLVAFNAATERLLGGALGGRLG
                     QPLVDIVPVFADAVLHEVACAVAA"
BASE COUNT          285 a          785 c          736 g          282 t
ORIGIN      
        1 tagccgatca gcgaccgggt gatccgcccg gcatgatcga ccagctccgc cgcccgggcg
       61 accagcgccg gatcagcccc cgcgctgtcc accagcgcga ccacggcggc gagggcgctc
      121 agggtattcc cgaggtcgtg cgcgaccccc gcggtgatcc ggccgagcag ccggagcgga
      181 tcgtcgtccg cgtcgccggc gccggtcgct tgctccgggg ccgaccgggc gagcgcggcc
      241 accacctcgc acagctcgtc gaggctcggc ccggccgcga cgaaccggat cgccccggcg
      301 gtgacctgcc ggcgccagac ttcgctggcc ggcgatggtc cgaccaccac gatgcgcgac
      361 gtcggcgaga gctgccgcgc cgcctcgatc agatcgagcc cgctggcgtc ggcgagctcg
      421 acgcccgtga cgacgacgtc gggcgtgtcg cggcacagcg agcgcaccgc ctcgcgcagc
      481 tccgccaccg caccctgcac cgcgatcgta tcgatccgcc cgagcgcctc cacgatcgcc
      541 tgccgcgccg ccctcgactt ctctgcaacc aggatattca ccatgtgcgg tctttgacgc
      601 catgtgcgcg aaaagcactg tcaggcaagc ctcgatcatg gtgtcggacg cggccggatc
      661 ttcacgtcag tcgccgtcct acaggcgccg ggcggtgacc ggggcgctga tcagaccagc
      721 ccgttctgga gcgcgtagcg cgtgagctcg gcgttgctcg ccatgcccat cttgtcgagc
      781 aagcgcgcgc ggtaggtgct gacggtcttg gcgctgaggt gcatgcgctc ggcgatctcg
      841 ccgacggtct cgcccgacgc gatgccgcgc atgacctcga actcgcgctg cgacagccga
      901 tcgtgcggcg cgccctgctc gccaccggcc gacgccgcga gccgctcggc gacggcctcg
      961 gtgatgtagc gcccgccgcg cacgatcctc tcgaacgcgg tgatcagctg ctcgggtgcc
     1021 gcggacttgg tgaggtagcc ggcggcgccg gcgcgcagcg cgcggaccgc gaactgctcc
     1081 tcgccgtgca ggctcatgat caggaccggc agccggggat agcggtgctt gatctcggac
     1141 agcaggtcga gcccgctgcg cccgggcatc gacaggtcga ggatcaccgc gtcgaacggc
     1201 cggctcagcg cggcgagcgc ctcgtcgccg ccgccggcct cggcgacctc gatgccggcg
     1261 aaccgatcgt cgaggatcgc gcgcaggccc ttgcgcacga cctcgtgatc gtcgacgagc
     1321 aggacccggt acatggcgcg gcgccatggt agcatcgcgc gcatggccga cggggtggaa
     1381 cgcgggccat cgcccggatc ctcgccaggg gacccgcccg ggcgtacgcc gggctacgcg
     1441 ccgggacgca cgatcgagcg ggagctgcgc cgccagctcg acaccaccca gtcgatcacc
     1501 cacatcggca gctgggagtg gaagatgggc ggcggcgcgg tgacctggtc cgacgagctg
     1561 taccggatct acgggctcga gccgcgctcg atcccggtca ccatcgagct cttcctgtcg
     1621 cggatccacc gcgacgaccg cgaccggatc gagcgcgaga tccagagcgc gctcgtccac
     1681 ccgggcaggt tcgcctaccg cgagcggatc gtgcggcccg acggctcggt gcgcacgctc
     1741 gacacgatcg gcgaggcgat cgccgacgag cgcggaacga ccacccggct ggtcggcacc
     1801 tgcctcgacg tgaccgaggc ggtcgcgcgc gaggcgcgga tccggttcta cgccgacgtg
     1861 ttcgagtacg ccgagatcgg gctgtcggcg ttccagctcg atcagccccc cggtcacacc
     1921 cccgatctcc cgcccgggcc gccggtgctc cggctggtcg cgttcaacgc cgcgaccgag
     1981 cggctgctcg gtggcgcgct cggcggccgg ctcggccagc cgctggtcga catcgtgccg
     2041 gtgttcgccg acgccgtgct ccacgaggtc gcgtgcgcgg tcgcggcc
//
LOCUS       DASVOR010000916         4151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447319,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000916 DASVOR010000000
VERSION     DASVOR010000916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447319"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF516_17770"
     CDS             complement(<1..186)
                     /locus_tag="VF516_17770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109587.1"
                     /translation="MDITTERPAVEAAAPGPAASPAVADRHREKVARIGEQLRALPPG
                     RPVSLRKASVSHQVPKRN"
     gene            complement(263..685)
                     /locus_tag="VF516_17775"
     CDS             complement(263..685)
                     /locus_tag="VF516_17775"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8109588.1"
                     /translation="MPVDIPTPEAVNQFFAREFPSAHAAGYRCIETGAGFAVARWPYD
                     ATQLRPGNLIAGPTQFAVGDLAFWFATFTVVGLAPMAVTSELYISFLRPAKGGDLLAR
                     AELLRAGKTKISGVVRVWVDGAPDRPVSHLTGSYVHIQ"
     gene            complement(714..1274)
                     /locus_tag="VF516_17780"
     CDS             complement(714..1274)
                     /locus_tag="VF516_17780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109589.1"
                     /translation="MRTRHRVAGLLAIAVLAIALPASAQPGMTAPTNPPPVGPGAGPA
                     TGACIACEAPVERQGPFLSFGLDAAPHGVPVGDVQLGWMLAPWIGVFVSLDGVVAEDT
                     RAHLWGAGLRLASGVAFAEVRILSVSNDGECYDGGCNDTPRIVMFGGGVELVRGQHAA
                     LDLHLHVLTDGRDAVPLVGLGLGFYF"
     gene            1695..2606
                     /locus_tag="VF516_17785"
     CDS             1695..2606
                     /locus_tag="VF516_17785"
                     /inference="COORDINATES: protein motif:HMM:NF020269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GON domain-containing protein"
                     /protein_id="HEX8109590.1"
                     /translation="MLSSFSFVAAAALGAACGDVPSQAHGMTPDAGPDATPPITCSNL
                     TCDANATCAMTDTGAACACKSGFTGDGKTCQDIDECTTSNGGCAVGCQNTPGSFQCYV
                     PATCTDIKAHDPGAADGNFTLYLDGDPTKPWTAFCAGLTTAPAEYLSVSGPNNSAYAS
                     GGASPGSTVVTKFTRVRFDPASHKIDISDRTFATSFGSVNHGGSGTMVTSMPYGVAMD
                     CRGATSQAGQANIDLSGTRFALDAATIFLTSGNQPGGMAQISTDHRRALLTGGGNCGW
                     TAPAGTPINPFNNNVTDGALLKLVYTP"
     gene            3323..3898
                     /locus_tag="VF516_17790"
                     /pseudo
     CDS             3323..3898
                     /locus_tag="VF516_17790"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
BASE COUNT          643 a         1474 c         1460 g          574 t
ORIGIN      
        1 gttccgcttg ggcacctggt gcgagaccga cgccttgcgc agcgacaccg ggcggccggg
       61 cggcagcgcg cgcagctgct cgccgatccg cgcgaccttc tcgcgatgcc ggtcggcgac
      121 cgccgggctc gccgcggggc cgggcgcggc ggcctcgacg gccggccgct cggtggtgat
      181 atccatacgg aatagtcgta gcaatcgcgc gcggactgtc acgccggtgt tgcgtgattt
      241 cgccgcgagc ccgctgcgtg cgtcactgga tgtggacgta cgagccggtc agatgcgaca
      301 ccgggcgatc gggcgcaccg tcgacccaga cccgcacgac gccgctgatc ttcgtcttgc
      361 cggcgcgcag cagctccgcg cgcgcgagca gatcgccacc ctttgccggc cgcaggaacg
      421 agatgtacag ctccgaggtc accgccatcg gcgcgagccc gaccaccgtg aacgtcgcga
      481 accagaacgc gaggtcgccg accgcgaact gggtcgggcc ggcgatcagg ttgcccggcc
      541 ggagctgggt cgcgtcgtac ggccagcggg cgaccgcgaa ccccgcgccg gtctcgatgc
      601 agcgatagcc ggcggcgtgc gccgacggga actcgcgcgc gaagaactga ttgacggctt
      661 cgggggtcgg gatgtcgacg ggcacgcccg accatatcgc agccggcgcg cgctcagaag
      721 tagaagccca ggccgaggcc gacgagcggg accgcatcgc gcccgtcggt gagcacgtgg
      781 aggtggaggt cgagcgcggc gtgctggccg cggacgagct cgacgcctcc tccgaacatc
      841 acgatgcgcg gcgtatcatt gcacccaccg tcgtagcact cgccgtcgtt gctgaccgac
      901 aggatccgca cctcggcgaa ggccactccg ctcgccagcc gcagcccggc gccccacagg
      961 tgagcccggg tgtcctcggc caccacgccg tcgagcgaca cgaacacgcc gatccagggc
     1021 gcgagcatcc agccgagctg cacgtcgccg acgggcaccc cgtgcggcgc cgcgtcgagg
     1081 ccgaacgaga ggaacggccc ctggcgctcc accggggctt cgcacgcgat gcaagcgcct
     1141 gtcgccgggc cggcgccggg gccgaccggc ggcgggttcg tgggggcggt catccccggc
     1201 tgggcggacg ccgggagggc gatggcgagg acggcgatcg cgagcaggcc ggcgacacgg
     1261 tggcgggtgc gcatcgcgcc agccgtactt caagcatcat gccgccgccg atcggcgcga
     1321 tcgcgtgggc cgcggtggcg accgccgcga caaccttgcc gcggccacga cagcgatgtc
     1381 gcggcgcgcg accgacccgg caagctctgg ctggcatcgg tcggcagccg cggctccgca
     1441 tgcagcgccc ggagaacgcg tgcaagcgag ctcgtctcgc gttggcgtcg cgcgatcgtg
     1501 gctcgactgc cgaaggccgg ccgcgctcgc cgggccgatg gcggccgcgt cagtgactcg
     1561 gcggcggcgc cgcagctgcg gtacctggcg acgacgtggc gcaatcggat catcggttgg
     1621 cgcgtccggt cgctgcgcgt gttccgcgcg atggtcatgg tggtctgcca ggagaactca
     1681 tgacgacgcg aaccatgctc tcgagcttca gtttcgtcgc cgcggcagcc cttggcgcgg
     1741 cgtgcggcga cgtccccagt caggcccacg gcatgacgcc tgacgcaggt ccggatgcga
     1801 cgccgccgat cacctgcagc aacctgacct gcgatgcgaa tgcgacctgc gcgatgaccg
     1861 acaccggcgc ggcctgcgcg tgcaagtcgg gcttcaccgg cgacggcaag acctgccagg
     1921 acatcgacga gtgcaccacc agcaacggcg gctgcgccgt gggctgccag aacacccctg
     1981 gcagcttcca gtgctacgtg cccgcgacct gcacggacat caaggcgcac gatccggggg
     2041 cggccgacgg caatttcacg ctctacctcg acggtgaccc gaccaagccg tggaccgcgt
     2101 tctgcgccgg cctgacgacg gcgccggccg agtacctgtc agtctccggg ccgaacaact
     2161 cggcgtacgc gtccggcggc gcgagcccgg gctcgaccgt ggtgaccaag ttcacccgcg
     2221 tgcggttcga cccggccagc cacaagatcg acatctccga tcgcacgttc gcgacctcgt
     2281 tcggctcggt caaccacggc ggcagcggga cgatggtcac gagcatgccg tacggtgtgg
     2341 cgatggactg ccgcggtgcc accagccagg ccggccaggc caacatcgat ctcagcggga
     2401 cgcggttcgc gctcgacgcc gcgaccatct tcttgaccag cggcaaccag ccgggcggta
     2461 tggcccagat cagcaccgac caccggcgtg cgctgctgac cggcggcggc aactgcggct
     2521 ggaccgcgcc cgccgggacg ccgatcaatc cgttcaacaa caacgtgacc gatggcgcgc
     2581 tgctgaagct cgtctatacc ccgtgagccc gctctgcgcc cggcgcggaa agtagcccgc
     2641 ggcgattccg acgcatcttg ggtgggcgcg ctgacgcagt tgcagagcta cgggggccgc
     2701 gctgcggcga cgcgttcacc gtcactgaaa tgcacgcacg cattgcaagt tcctgccgcc
     2761 ggggcgcccg ggccgaccgg gggcgggttc gcggggcggt catgcccggc gggtcggcgg
     2821 acgcggggag ggcggtggcg aggacggcga tcgcgaggac gccggtgacg cggcgccggg
     2881 tgcgcatcgc gccagctgca cttcacgcac catgccgccg ccgaccggcg cgatcgcgtg
     2941 ggccgcggtg gtgaccgccg cgacaatccc ctccaccctg cggacagcga tgtcgcggcg
     3001 cgcgcccggc ccggcaagtt ctggctggca tcggccggtg gtcgcgggat ttcggcgggg
     3061 gggaccgatg gcgatcatcg cgaggttgac gtcgcgtcga gcagggtcgc tgacacaagc
     3121 tgcagcccgc gtgtctcagg cgcgcagccg gcgtcacggg tccgcggacg ccccttgaac
     3181 ctcacgcgaa gttgctccaa gcatgtgagc gtgcggcagg tttccggaga ggcgcaatga
     3241 ccgacggggg tgcgcgcgat ccgctgatcg ggagcttgtt caacaactac cggatcgacg
     3301 cgttgctcgc tcagggcggc atggggcagt gtacctgctc cgcgacgcga ccctgccgaa
     3361 catccgcaag gtgctcaagg tcatcctgcc ggagtttgcg aacaacgcgt ccatcaggga
     3421 gcggttcctc cgtgaggccg aggcggtttc gatgctccgc catcccaacg tcgtcggcat
     3481 cgacgcgatc ggtacgctcc ccgatggccg gctgtgcatg gtgatcccgt tcctggacgg
     3541 caagccgctc gatcaattcc tcgccgagca tggcggccgg ctggcgccgc accgggtgat
     3601 gcacatcctc gcgcatatcg ctcgcggcct cgaccatgtg caccgccagg ggatcatcca
     3661 tcgcgatctc aaaccgggaa acgtcttcgt cgagccgacc gaggatgacc ggtacttctg
     3721 caaggtactt gatttcggca tcgcgaagca gctgaacgcc aggtccaagc gcctgtctcc
     3781 gacgctttcg gcgcctaccg gaaccccatg ctacatggcc gtcgagcagt acgtcagtcc
     3841 aggggattac tcggcctggc cgccgctggt tccgttggtg tcgcggcact cgcgctgagc
     3901 gccacccgag atagcagcat gactccggcc gcggaggcgg tccggcaacc gctcgagagc
     3961 tcgacgtcgt cgccgactcc gtccataccc acccgaggaa tcaaccgtgc gcgagaatcc
     4021 gcagcgggga ccagtgccgt cgttcccgcc acggcggcct cagtcgaggc aacatctacc
     4081 agctcaacat cgaagacggc ggcgccgtcc gtcgccgcgg atctcccgcg cgcgaccagc
     4141 gcagcaccga t
//
LOCUS       DASVOR010000917         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67499,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000917 DASVOR010000000
VERSION     DASVOR010000917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67499"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /gene="purH"
                     /locus_tag="VF516_17795"
     CDS             complement(<1..508)
                     /gene="purH"
                     /locus_tag="VF516_17795"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829998.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEX8109591.1"
                     /translation="MLRALVSVHDKTGVIELARVVHALGGEILSTGGTAAALSAAGIP
                     VTQVSAHTGAPEILDGRVKTLHPRIHGGLLGRATPAHQQEMAAHAIPPIDLLVANLYP
                     FEATIARPGTSLHDAIENIDIGGPAMLRSAAKNHERVAVVVDPADYGWVGEALRAGGP
                     SPAQRLALA"
     gene            complement(549..1139)
                     /locus_tag="VF516_17800"
     CDS             complement(549..1139)
                     /locus_tag="VF516_17800"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8109592.1"
                     /translation="MNHRDDEDEDELEGPDDADDADDTALADGDGGDRATAAADMPEV
                     SRKVRRRRRRTRPRSKTIAMKRLTREELRQGALMYPPVDIPRPTSRAECREEMRPCPW
                     VACKHHLYLDINPETGSIKINFPDLEPWELRHTCALDVAERGGITLEEVGEIMNLTRE
                     RIRQVEVRGLLKLKMGSPSPDELGADLLAGKVQLDN"
     gene            complement(1228..>1827)
                     /locus_tag="VF516_17805"
     CDS             complement(1228..>1827)
                     /locus_tag="VF516_17805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109593.1"
                     /translation="ADIARRRDDDAPRAGAAPARAALLQEEELRAIEGAYPDGITAVQ
                     IVDAFTSRGIKFSEASFRKYVQQGLLPRSRRVGRKGKHRGSLGVYPSKTVRRINAVKQ
                     LMVDGYTIEEIQGQFLLYTDLVEGVAEHLAELWSRLTADATKLDPAIRRELEQKLADA
                     RRDGDRLVERLGELTRRFAAPRTDSLRLAGAAEGAEDLL"
BASE COUNT          218 a          659 c          659 g          291 t
ORIGIN      
        1 gtgcgagcgc cagccgctgc gccggcgacg ggccaccggc ccgcagcgcc tcgccgaccc
       61 agccgtagtc ggccgggtcg accacgaccg cgacccgctc gtggttcttc gcagccgagc
      121 gcagcatcgc cggtccgccg atgtcgatgt tctcgatcgc gtcgtgcagc gaggtccccg
      181 gccgggcgat cgtcgcctcg aacgggtaca ggttcgccac cagcaggtcg atcggcggga
      241 tcgcgtgcgc cgccatctcc tgctggtgcg ccggcgtggc gcggccgagc aggccgccgt
      301 ggatccgcgg gtgcagcgtc ttgacccggc cgtcgaggat ctcgggcgcg ccggtgtgcg
      361 ccgagacctg ggtcaccggg atcccggccg cggacagcgc cgccgccgtg ccgccggtcg
      421 acaggatctc gccgccgagc gcatggacca cccgggccag ctcgatcacg cctgtcttgt
      481 cgtgaacgga aaccagcgcg cgcaacatgc cgcgcgtata ccaaccgtga cgcggaccgt
      541 ctaccgggtc agttgtcgag ttgcaccttg ccggcgagca gatcggctcc gagctcgtcg
      601 ggcgacggcg atcccatctt cagcttgagc aaaccgcgga cctcgacctg ccggatccgc
      661 tcgcgggtca ggttcatgat ctcgccgacc tcttccagcg tgatcccgcc gcgctcggcg
      721 acgtcgagcg cgcaggtgtg cctgagctcc cacggctcga ggtcggggaa gttgatcttg
      781 atcgatccgg tctcggggtt gatgtcgagg tagaggtggt gcttgcacgc gacccacggg
      841 cacggccgca tctcctcgcg gcactcggcg cgcgacgtcg gccgcgggat gtcgaccggc
      901 gggtacatca gcgcgccctg gcgcagctcc tcgcgggtca gccgcttcat cgcgatcgtc
      961 ttgctgcgcg gccgggtccg ccggcggcgg cgccggacct tgcggctgac ctcgggcatg
     1021 tcggccgcgg cggtcgcgcg atcgccgccg tcgccgtccg caagggcggt gtcgtccgcg
     1081 tcgtccgcgt cgtctggccc ctcgagctcg tcctcgtcct catcgtcacg gtggttcatg
     1141 gcgaaatctc ctgatctgac ggcgcaccgg gttgcgcgtc gcgtcgcgcg gccgggctgc
     1201 ctcgggtgac ggtccatgcg gtcatgttca gagcaagtcc tcggcgccct cggcggcgcc
     1261 ggcgagacgg aggctgtcgg tgcgtggggc ggcgaaccgc cgggtcagct cgccgagccg
     1321 ctcgaccagc cgatcgccgt cgcgccgggc gtcggcgagc ttctgctcga gctcgcgccg
     1381 gatcgccgga tcgagcttgg tggcgtcggc ggtgagccgc gaccacagct cggccaggtg
     1441 ctcggcgacg ccctcgacca ggtcggtgta gagcaggaac tgcccctgga tctcctcgat
     1501 cgtgtagccg tcgaccatca gctgcttgac ggcgttgatc cggcgaaccg tcttggacgg
     1561 gtagaccccg agcgagcccc ggtgcttgcc cttgcggccg acccggcggc tgcgcggcaa
     1621 cagcccctgc tggacgtact tgcggaagct cgcctcggag aacttgatgc cgcggctggt
     1681 gaacgcgtcg acgatctgca ccgcggtgat cccgtcgggg tacgcgccct cgatcgcgcg
     1741 cagctcttcc tcctgcagga gcgcggcgcg cgccggggcg gcaccggcac gcggcgcgtc
     1801 gtcgtcgcgg cgccgcgcga tgtcggc
//
LOCUS       DASVOR010000918         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_125957,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000918 DASVOR010000000
VERSION     DASVOR010000918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125957"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF516_17810"
     CDS             complement(<1..550)
                     /locus_tag="VF516_17810"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8109594.1"
                     /translation="MPTTAHDGGAPDVLSRALGVFGHRSLRPGQAEVISDVLSGRPVV
                     AVMPTGSGKSLCYQLPAVVLAERGHVTLVVSPLIALMKDQVDALTALGVPAVALTSAA
                     GADEQREILDGIRAGIYTLIYVAPERFRSPRFVDTLRATAGRLGLIAIDEAHCISEWG
                     HDFRPDYRRLGDAVRLLGPPRIA"
     gene            912..>1859
                     /locus_tag="VF516_17815"
     CDS             912..>1859
                     /locus_tag="VF516_17815"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8109595.1"
                     /translation="MHDRTTVVPSSRVGGIDLSRGSVTDRPWGVTLASLGLCGRTGQL
                     TVRSTDDKRFRIAFRDGVVVGATSPLAVDSVARIALTGHLVTSSQVAEIARRVAAAPD
                     RDEISIVAEAARLAPDQIDRLRRRAIVQRAARTFTVEAGTLTFDDRITIPVAPHSEID
                     VRGVIYLGARLHLSEQRLTEDVRRFGARFIMKAEAHPALARFDFTTVEYPIVEALERG
                     TSLPEIEAAHREIDPRTARAVLYALAACQAIAQLDGESLERSETGGGDPDGHAGGAGG
                     RASRGIEIPARPPLEAREPTVSRTPTLRAPTMTRVPTPRE"
BASE COUNT          277 a          668 c          652 g          262 t
ORIGIN      
        1 ccgcgatccg cggcgggccg agcagccgca ccgcatcgcc gagccggcgg tagtcgggcc
       61 gaaagtcgtg tccccactcg gagatacagt gagcctcgtc gatcgcgatc aggccgagcc
      121 ggccggccgt ggcgcgcagc gtgtcgacga accgcgggct gcggaaccgc tcgggcgcga
      181 catagatcag cgtgtagatc ccggcgcgga tgccgtcgag gatctcgcgc tgctcgtcgg
      241 ccccggccgc cgaggtcagg gcgaccgcgg ggacgcccag ggccgtgagc gcgtcgacct
      301 ggtccttcat cagcgcgatc agcggcgaga ccaccagcgt gacgtggccg cgctcggcaa
      361 gcaccaccgc aggtagctga taacacaggg acttcccgga tcccgtgggc atcaccgcca
      421 cgaccggccg gcccgacagc acgtcggaga tcacctcggc ctggccgggc cgcaacgagc
      481 ggtggccgaa caccccgagc gcccgcgaca gcacgtcggg ggcgccgccg tcgtgtgcgg
      541 tggtcggcat ctccagcacg gcggccaccc tagcagagcg gtacgacatc cgaaactatc
      601 gagatcatgt ccgaaattcg gacacccctg tccggctgtc cgggccgctc acagcgatcg
      661 ccgccgcgct tgccgccgcg tcctcgacgg ggccggtgcc gccgggcgcg ctcggccggg
      721 tcgtgcccga agctactttg gttcacgtga acattcatta tccgacctta cgaactggcc
      781 aaaatatgta cgacatcata ctcgcgttag ataggttgat ttcacattca acgtaaattc
      841 ttctacggga tgtaatcctt gggcgcagct tttcgttgcg ttcctcgcgc ggacaccgtt
      901 caattcagcc catgcacgac cgcacgaccg tggtgccgtc gtcccgggtg ggtgggatcg
      961 atctatcccg aggcagcgtc accgatcgcc cctggggcgt gacgcttgcc tcactgggcc
     1021 tctgcggccg gaccgggcag ctcacggtgc gctcgacgga tgacaagcgg ttccggatcg
     1081 cgttccggga cggcgtcgtg gtcggcgcga cctccccgct ggcggtggac tcggtcgcgc
     1141 ggatcgcgct gaccggccac ctggtgacct cgtcgcaggt cgccgagatc gcccggcggg
     1201 tcgccgccgc ccccgatcgc gacgagatct cgatcgtcgc cgaggccgcc cgcctcgcgc
     1261 ccgatcagat cgaccggctg cgccggcggg cgatcgtgca gcgcgcggcg cggacgttca
     1321 cggtcgaggc cggcacgctg acgttcgacg accggatcac gatcccggtc gcgccgcaca
     1381 gcgagatcga cgtgcgcggc gtgatctacc tcggcgcccg gctccacctg tccgagcagc
     1441 ggctgaccga ggacgtgcgg cggttcggcg cgcgcttcat catgaaggcc gaggcccacc
     1501 ccgcgctggc gcgcttcgac ttcacgaccg tcgagtaccc gatcgtcgag gcgctcgagc
     1561 gcggcaccag cctgcccgag atcgaggccg cgcaccgcga gatcgatccg cggaccgcgc
     1621 gcgccgtgct ctacgcgctg gcggcgtgcc aggcgatcgc gcagctcgat ggcgagtcgc
     1681 tcgagcgaag cgagacgggg ggtggggacc ccgacggcca cgccggcgga gcggggggca
     1741 gagcctcccg tgggatcgag atcccggccc ggccgccgct cgaggcgcgg gagccgacgg
     1801 tgtcgcgcac gccgaccctg cgcgcgccga cgatgacccg ggtgccgacg ccgcgggag
//
LOCUS       DASVOR010000919         5157 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_505835,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000919 DASVOR010000000
VERSION     DASVOR010000919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5157
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505835"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..779
                     /locus_tag="VF516_17820"
     CDS             12..779
                     /locus_tag="VF516_17820"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8109596.1"
                     /translation="MAHADGVLHLAAVSRVLWGEREPERCWSTNVGGLGNILALAATA
                     PARPWIIFASSREVYGQPDRLPVTEDWPVRPVNTYGRSKAAGEQLVAAARRDGVRACT
                     IRLSNVFGSTEDHADRVVPAFARAAALGRELRVDGAGHTFDFTHVDDVARGIATLAEL
                     LAMNEVVPPPIHLVSGTPTTLGELASAAIRIAGAGSSVRHAPPREFDVARFVGSPARA
                     KALLGWSPRISLEEGLARLIRAFRDLKDAARAEEVAS"
     gene            776..3127
                     /locus_tag="VF516_17825"
     CDS             776..3127
                     /locus_tag="VF516_17825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8109597.1"
                     /translation="MKILKVIHGYPMRYNAGSEVYSQTLCHGLAERHEIHVFTREEDA
                     FAPDFRLRVERDEDDPRITVHVVNNPRLKDRYRATGVDQRFAEVVERVRPDLVHVGHL
                     NHLSTSLLREAALREVPIVFTLHDYWLMCPRGQFMQMFPEDPNDLWAACDGQDDRKCA
                     ERCYARYFSGAPDEHELDVGYWTDWVGRRMRHVREMAELVDRFIAPARYLQARYRDAF
                     GLPEHKLVYLDYGFARERLSGRRRVPGEPFTFGYIGTHIPAKGIHQLIRAFGRLAGDA
                     RLRIWGRSRGQDTEALKGIAASLPARAAGRVEWLPEYKNLEIVRDVFDRCDAIVVPSV
                     WVENSPLVIHEAQLARVPVITANVGGMAEYVRHEVNGLLHEHRSIASLAAQMQRLVDD
                     PVLATRLGARGYLFSDTGDIPDVRDHVRDVERLYEQVLRRHASARVPRRSGPWRITFD
                     TNPDTCNLHCVMCEEHSPHSPLQLRRRAEGRPRRVMPFAMIERVVAEAVPHGLREIIP
                     STMGEPLLYEHFEDILELCHRHGIKLNLTTNGTFPRLGAEEWARRIVPVTSDVKISWN
                     GATKATHEAIMLGSSWDKVVDNVRAFVAVRDAHASAGGDRCRVTFQLTFLESNVAELA
                     DMVRLAVALGVDRVKGHHLWAHFDQIQGESMRRSAESIGRWNAAVLAAREAAAERPLP
                     SGEQILLENLFLLDAAATEDLAPGGPCPFLGQEAWVSAAGRFDPCCAPDAQRRTLGEL
                     GSVTDRSFMEIWNGAPYRELTSSYRNRRLCLGCNMRRPAGGEP"
     gene            3142..4800
                     /locus_tag="VF516_17830"
     CDS             3142..4800
                     /locus_tag="VF516_17830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8109598.1"
                     /translation="MAAPDGSHHTTPGGIPAYRERYDAVLAFHAPGLAAVCRGGEAWH
                     IRPDGSAAYERRFTRTFGCYSGLAAVIHTSGWHHITPEGRDAYSERYTWCGNFQEGHC
                     TVREAGGAYLHIAADGRAVYPERWRYAGDYRDGIAVVQAADGRSTHIDRNGGLVHERW
                     FLDLDVFHKGFARARDEDGWTHVDRTGRPVHARRFAMVEPFYNGQARVERFDGALEVI
                     DESGTTVAELRPPRCSEFAALSGDLVGFWRTQTIAAAVRLGVIEALPATEADVAQRCC
                     LRRGGAHRLLRALGELRLATRDGERWQLTPRGTFLRAGHPLTLADAALEYAGPFSDMW
                     RGLADALRAGSDWTAPDVFGHVARDELRRAGHHRMLQSYARHDYQEVARALDLRGDER
                     IVDAGGGLGTLARLVLEAYPSVQITVLDRPEVIEQARRHGPPLRGLDWRSADLFAPWG
                     LHVDVVLLSRVLHDWDDDGARRILCHARAALPTGGRVFVIEMLLPEGSVAGGLCDLHL
                     LMATGGQQRSAQEFERLFGAAGFELQRVRTVCSLPSILVGEATT"
     gene            4838..>5157
                     /locus_tag="VF516_17835"
     CDS             4838..>5157
                     /locus_tag="VF516_17835"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109599.1"
                     /translation="MRVACLDLPGFPLQLVWRQEPAWQTQPVVVIDSDRPQGVVLWAC
                     ERARASGVLVGQRYAFALSLCGGLRARVVSDAELAEAITELRAALHRLSPGVEPGEPG
                     TFWL"
BASE COUNT          720 a         1852 c         1813 g          772 t
ORIGIN      
        1 ttcgcgaggc gatcgcgcat gccgatggcg tcctccacct cgccgccgtg tcgcgcgtcc
       61 tgtggggcga gcgcgagccc gagcggtgct ggtcgaccaa cgtcggcggc ctgggaaaca
      121 tcctggcgct cgcggcgacc gcgcctgcgc gcccctggat catcttcgcg agcagccgcg
      181 aggtgtacgg ccagcccgac cgcctgccgg tcacggagga ttggccggtt cgccccgtga
      241 acacctatgg gcgctcgaag gccgcgggcg agcagctcgt cgccgcggct cggcgagatg
      301 gcgtacgcgc gtgcacgatc cgcctgtcga atgtgttcgg ctcgacggaa gatcatgccg
      361 atcgcgtggt gcctgcgttt gcgcgtgccg cggcgctcgg ccgcgagctg cgcgtcgatg
      421 gcgccggcca caccttcgat ttcacgcatg tcgacgacgt cgcgcgcggc atcgcgaccc
      481 tcgccgagct cctcgccatg aacgaggtcg tcccaccccc catccatctc gtcagcggca
      541 caccgacgac gctcggcgag cttgcaagcg cggcgatccg catcgcggga gctggatcga
      601 gcgttcgcca cgcgccgccg cgggaattcg acgtcgcgcg cttcgtcggc agccccgcca
      661 gggccaaggc gctgctcggc tggagcccac gcatcagcct cgaggagggg ctcgctcgac
      721 tgatccgcgc gttccgcgac ctgaaggacg cggcccgggc cgaggaggtc gcgtcatgaa
      781 gatcctgaag gtgatccacg gctacccgat gcgctacaac gccgggtccg aggtctacag
      841 ccagacgctg tgccacgggc tcgcggagcg tcacgagatc cacgtgttca cgcgcgagga
      901 ggacgccttc gcgcccgact tccgcctccg cgtcgagcgc gacgaggacg acccgcgcat
      961 caccgtccac gtcgtcaaca atccgcggct caaggaccgc tatcgcgcca ccggcgtcga
     1021 ccagcgcttc gccgaggtcg tcgagcgggt gagacctgac ctggttcacg tcggtcacct
     1081 caaccatctc tcgacgtcgc tgctgcggga ggccgcgctg cgcgaggttc cgatcgtgtt
     1141 cacgctgcat gactactggc tgatgtgccc gcgcggccag ttcatgcaga tgttccccga
     1201 ggacccgaac gacctgtggg cggcctgcga cgggcaggac gatcgcaagt gcgccgaacg
     1261 ctgctacgcg cgctacttca gcggcgcgcc cgacgagcac gagctcgacg tcggctactg
     1321 gaccgactgg gtcggccggc gcatgcgcca cgtgcgggag atggccgagc tcgtcgatcg
     1381 cttcatcgcg cctgcgcgat acctccaggc tcgctaccgg gacgcgttcg ggttgccgga
     1441 gcacaagctc gtctacctcg actacggctt cgcgcgcgag cggctgagcg gccgccgccg
     1501 cgtacccggc gaaccgttca cgttcggtta catcgggacg cacatcccgg cgaagggcat
     1561 ccaccagctc atccgggcgt tcggacggct cgccggcgat gcgcggcttc gcatctgggg
     1621 ccggtcccgc ggtcaggaca cggaagcgtt gaagggcatc gccgcgtcgc tgcccgcgcg
     1681 cgccgccggc cgcgtcgagt ggctccccga gtacaagaac ctggagatcg tccgcgacgt
     1741 cttcgaccgc tgcgacgcca tcgtcgtccc gtcggtctgg gtcgagaact cccccctggt
     1801 catccacgag gcccagctgg ctcgcgtccc cgtcatcacc gcgaacgtgg gcggcatggc
     1861 cgagtacgtg cgccacgagg tcaacgggct gctccacgag caccgctcga tcgcgtccct
     1921 cgcggcgcag atgcagcggc tggtcgacga cccggtgctc gcgacccgcc tcggtgcccg
     1981 cggctacctg ttcagcgaca cgggcgacat tcccgacgtg cgcgaccacg tccgcgacgt
     2041 cgagcggctc tacgagcagg tgctgcgccg ccacgcctcc gcccgcgtcc cgcgccggag
     2101 cggtccgtgg cggatcacct tcgacaccaa ccccgacacc tgcaacctcc actgcgtgat
     2161 gtgcgaggag cactctccgc acagcccgct tcagctccgg cgaagggccg agggccgacc
     2221 gcgccgggtg atgccgttcg cgatgatcga gcgcgtcgtc gccgaggcgg tgccacatgg
     2281 cctgcgagag atcatcccgt cgacgatggg cgagcccctg ctctacgagc acttcgagga
     2341 catcctggag ctctgccatc gccacgggat caagctgaac ctcaccacca acgggacgtt
     2401 tccgcgcctc ggcgccgagg agtgggccag gcggatcgtg cccgtgacgt ccgacgtcaa
     2461 gatctcgtgg aacggcgcga ccaaggccac ccacgaggcg atcatgctgg gcagcagctg
     2521 ggacaaggtc gtcgacaacg tacgcgcgtt cgtggccgtg cgcgatgctc atgcatccgc
     2581 cggcggagat cgctgccgcg tcacgttcca gctcaccttc ctcgagtcga acgtcgccga
     2641 gctcgccgac atggtccgtc tggccgtcgc gctcggcgtc gaccgcgtca agggacacca
     2701 cctgtgggcg cacttcgacc agatccaggg cgagtcgatg cgacgcagcg ccgaatcgat
     2761 cggccggtgg aacgcggccg tgctcgccgc gcgcgaggcc gcggccgagc gaccgctgcc
     2821 gagcggcgag cagatcctgc tcgagaacct gttcttgctc gacgctgctg ccaccgagga
     2881 cctggcgccg ggcggcccgt gcccgttcct gggccaggag gcatgggtga gcgccgccgg
     2941 gcgattcgac ccgtgctgcg cgcccgatgc gcagcggcgc acgctcggcg agctcggcag
     3001 cgtcaccgac cgcagcttca tggagatctg gaacggcgcg ccataccgcg agctcacgtc
     3061 gagctatcgc aaccgccggc tctgcctcgg ctgcaacatg cgcaggccgg ccggaggcga
     3121 accatgagct gggagcgcct gatggctgcg cccgacggtt cgcaccacac gaccccgggc
     3181 ggcatccccg cgtaccgcga gcgctacgac gcggtgctcg cctttcacgc gccggggctc
     3241 gcagcggtgt gccgtggggg cgaagcctgg cacatccgcc cggacggttc ggcggcgtac
     3301 gagcggcgct tcacgcggac cttcggctgt tacagcggcc tggcggcggt catccacacc
     3361 agcggctggc accacatcac tcctgagggc cgcgacgcgt actccgagcg ctacacgtgg
     3421 tgcggcaact tccaggaggg tcactgcacc gttcgcgagg ccggtggcgc ctacctgcac
     3481 atcgcggccg acggtcgcgc cgtctacccc gagcggtggc gctacgcggg cgactatcga
     3541 gacggcatcg ccgtcgtgca ggcagccgac ggacgctcca cgcacatcga ccgcaacggc
     3601 ggcctggtgc acgagcgctg gttcctcgac ctcgacgtct tccacaaggg gttcgcccgg
     3661 gcgcgcgacg aggacggctg gactcacgtc gacaggaccg gccgccccgt ccacgcccgg
     3721 cggttcgcca tggtcgagcc gttctacaac gggcaggcgc gcgtcgaacg cttcgacgga
     3781 gcgctcgagg tgatcgacga gtcgggcacg acggtcgccg agcttcgtcc gccgaggtgc
     3841 tcggagttcg cggcgctgtc tggggacctg gtcggattct ggcgcaccca gaccatcgca
     3901 gccgcggtgc gcctgggcgt catcgaggcg ctgcccgcga cggaggccga cgttgcgcag
     3961 cgctgttgcc tccggcgggg cggcgcgcat cgcctgctgc gtgcgctggg cgagctgcgt
     4021 ctggccaccc gggacggcga acgatggcag ctcacgcccc ggggcacgtt tctgcgcgcc
     4081 ggccacccgc tgacgctggc cgatgcggcg ctcgagtatg cggggccgtt ctcggacatg
     4141 tggcgcgggc tggcagacgc gctgcgcgcc ggctcggact ggacggcccc ggacgtgttc
     4201 ggccacgtcg cccgggacga gctccggcga gcaggacacc accggatgct ccagagctac
     4261 gcccgccacg actatcagga agtggcgcgc gcgctcgacc tgcgcggcga cgagcggatc
     4321 gtcgacgccg gcggtggtct cggaacgctc gcgcggctcg tgctcgaggc ctacccgtcc
     4381 gtccagatca ccgtcctcga tcggcccgag gtcatcgaac aggctcggcg ccacggtccg
     4441 ccgctgcgcg ggctggactg gcgctcggcc gacctcttcg cgccgtgggg actgcacgtg
     4501 gacgtcgtcc tcctgtcgcg ggtcctgcac gactgggatg acgacggcgc gcgccgcatc
     4561 ctctgccatg ctcgcgccgc gcttccgacc ggcgggcgcg tgttcgtgat cgagatgctg
     4621 ctcccggagg gtagcgtcgc tggagggctc tgcgaccttc acttgctgat ggcgacggga
     4681 gggcagcagc gatccgctca ggagttcgag cgtctgttcg gcgccgccgg cttcgagctc
     4741 cagcgcgtcc gcacggtctg ctcgcttcca tcgatcctcg tgggggaggc taccacgtga
     4801 cctgaaccga tgtacagtag cagggtccaa ggatcgcatg cgcgtcgcct gtctcgatct
     4861 gcccggcttt ccgctccagc tggtgtggcg gcaggagccg gcgtggcaga cgcagccggt
     4921 cgtggtgatc gacagcgatc ggccgcaggg cgtggtgctg tgggcgtgcg agcgggcgcg
     4981 agcgtcgggg gtgctcgtgg ggcagcgcta cgcgttcgcg ctgtcgctgt gcggcgggct
     5041 gcgggcgcgg gtggtgtccg acgccgagct cgccgaggcg atcaccgagc tgcgcgcggc
     5101 cctgcaccgg ctgtcgccgg gcgtcgagcc cggggagccc gggacgttct ggctcga
//
LOCUS       DASVOR010000920         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_388843,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000920 DASVOR010000000
VERSION     DASVOR010000920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388843"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2542
                     /locus_tag="VF516_17840"
     CDS             <1..>2542
                     /locus_tag="VF516_17840"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8109600.1"
                     /translation="KLVVDLVPQLGLIVGPQPPVPELPLTESETRLRLVFGRLFAACA
                     TPDHPLAMFIDDMQWADTASLALIAHLLTDGDTRHLLIVGAYRDHEVDPAHPMVRALE
                     SARRSTARIRELVLGPLSEDDLGRLVADTVHASPAEAAPLAGLVRDKTGGNPFFAIQF
                     LTALHHKRLIWFDREAYRWQWDAARIRAEGYTDNIAELMRNRLYTLPGETQAALQRAA
                     CLGNTVDAATLAIACEREIAAALRPAIEHHLLFETVQGERRTYRFPHDRVHEAAYALV
                     PEPERVQVHLAIGRRLLASTPPEQLAGKVFEIVSQLDRGAARIESPAEREQLAELYLL
                     AGTRAQASTAYASALRYFTAGAALLAEVPEVHRPELAFALDLHRAECEHLTGALDAAE
                     PRLAELARRATSLVDLAAVTSARIALYTTLDRAALAVEATLEYLRRAGTAWSAHPTAD
                     EVRREYDRIWQQLGSRAIEDLADLPPMTDPERRATTELLALAQSPAKFVDENLYTLMV
                     CRAVNLSLEHGNSEASCWTYAALGSILRQRFGNAAAGFRFGQLGFDLVERRGLLRWKV
                     RVYVELCYRILPWTKQPRDGVELARRAFDAAMETGDLTFAAYACNAPVTLSLLAGAPL
                     TAILPESDAALAFMHKHKFGMMVDIVATNRQLIRSLRGMTRELGSFDDEAFAEAPFEQ
                     RLDAAPSFPACWYWMHKVQARFLAGDHAGAVAAAAKARPMLWRIPSFPESAEHVFYAA
                     LACAAHHDSAPDDERTRLRDQLAAHHAEIAAWAEHGPERFRDRAELTGAELARIRGDA
                     DHAERLYEQAIRTARAHGFVHIEAIAYETAARFHRARGQTLIADAYVRE"
BASE COUNT          351 a          954 c          885 g          352 t
ORIGIN      
        1 caagctggtc gtggatctgg tcccgcagct cgggctgatc gtcgggccgc aaccgccggt
       61 gcccgagctg cccttgaccg agtcggagac ccggctccgc ctggtgttcg gccggctgtt
      121 cgccgcctgc gccacgcccg accacccgct cgcgatgttc atcgacgaca tgcagtgggc
      181 ggacaccgcg agccttgccc tgatcgccca cctgctgacc gacggcgaca cccgccatct
      241 gctgatcgtc ggcgcctacc gcgaccacga ggtcgatccg gcgcacccga tggtgcgcgc
      301 gctcgagtcg gcgcggcgga gcaccgcgcg gatccgcgag ctcgtgctcg ggccgctgtc
      361 cgaggacgat ctcggccggc tggtcgccga caccgtccac gcctcgcccg ccgaggcggc
      421 gccgctcgcc ggtctggtcc gcgacaagac cggcggcaat ccgttcttcg cgatccagtt
      481 cttgaccgcg ctgcaccaca agcgcttgat ctggttcgat cgcgaggcgt accgctggca
      541 atgggatgcc gcgcggatcc gcgccgaggg ctacaccgac aacatcgccg agctgatgcg
      601 caaccggctg tacaccctgc cgggcgagac ccaggcggcg ctccagcgcg cggcgtgcct
      661 cggcaacacg gtcgacgccg ccacgctggc gatcgcgtgc gagcgcgaga tcgcagcggc
      721 gctacgccct gcgatcgagc accacctgct gttcgagacc gtccagggcg agcggcggac
      781 ctatcgcttt ccccacgacc gcgtgcacga ggcggcctat gcgctggtgc ccgagccgga
      841 gcgcgtgcag gtccacctcg cgatcggccg ccggctgctc gcgagcacgc caccggaaca
      901 gctcgccggg aaggtgttcg agatcgtcag ccagctcgat cgcggcgccg cgcggatcga
      961 gtccccggcg gagcgcgagc agctcgccga gctgtacctg ctcgccggta cgcgtgcgca
     1021 ggcctcgacc gcctacgcct cggcgctccg ctacttcacc gcgggcgccg cgctgctcgc
     1081 ggaggtgccc gaggtccacc gccccgagct cgcgttcgcg ctcgatctcc accgcgccga
     1141 gtgcgagcac ctgaccggcg cgctcgacgc ggccgagcca cggctcgccg agctcgcccg
     1201 ccgcgccacg agcctcgtcg acctggcggc ggtgacgtcg gcgcggatcg cgctctacac
     1261 gacgctcgat cgggcggcgc tcgcggtcga ggcgacgctc gagtacctgc ggcgcgcggg
     1321 caccgcgtgg tccgcgcacc cgaccgccga cgaggtccgg cgcgagtacg atcggatctg
     1381 gcagcagctc ggatcgcgcg cgatcgagga cctcgccgac ctgccgccga tgaccgatcc
     1441 ggaacgccgc gcgacgaccg aattgctcgc gctcgcgcag tcgcccgcga agttcgtcga
     1501 cgagaatctg tacacgctga tggtctgccg ggcggtgaac ctgagcctcg agcacggcaa
     1561 cagcgaggcc tcgtgctgga cctacgccgc gctcggatcc atcctccggc agcggttcgg
     1621 caacgccgcg gcggggttcc ggttcggcca gctcgggttc gatctggtgg agcgacgcgg
     1681 gctgctgcgc tggaaggtcc gcgtgtacgt cgagctctgc taccggatcc tgccgtggac
     1741 gaagcagccg cgcgacggcg tcgagctggc gcggcgcgcg ttcgatgccg cgatggagac
     1801 cggcgatctc acgttcgcgg cgtacgcctg caacgccccg gtcacgctgt ccttgctggc
     1861 gggcgctccg ctcacggcga tccttcccga gtccgacgcc gcgctcgcgt tcatgcacaa
     1921 gcacaagttc ggcatgatgg tcgacatcgt cgcgaccaat cgccagctga tccgatcgct
     1981 ccgggggatg acgcgggagc tcggctcgtt cgacgacgag gcgtttgccg aggcgccatt
     2041 cgaacagcgc ctcgacgctg caccgagctt ccccgcgtgc tggtactgga tgcacaaggt
     2101 gcaggcgcgg tttctggccg gcgatcacgc cggcgcggtg gcggcggcgg ccaaggcccg
     2161 gccgatgctg tggaggatcc cgtcgttccc cgagagcgcg gagcacgtgt tctacgccgc
     2221 gctcgcgtgc gcggcccacc acgacagcgc gccggacgac gagcggacgc ggttgcgcga
     2281 ccagctcgcc gcccaccacg ccgagatcgc ggcgtgggcg gagcacggcc ccgagcgctt
     2341 ccgcgaccgc gccgagctca ccggcgccga gctcgcgcga atccgcggcg acgccgacca
     2401 cgcggagcgg ctctacgagc aggcgatccg caccgcgcgc gcacacggct tcgtccacat
     2461 cgaggcgatc gcctacgaga cggcggcgcg gtttcatcgc gcgcgcggcc agacgctgat
     2521 cgccgacgcc tacgtgcgcg ag
//
LOCUS       DASVOR010000921         6188 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447329,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000921 DASVOR010000000
VERSION     DASVOR010000921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6188
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447329"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="VF516_17845"
     CDS             <1..652
                     /locus_tag="VF516_17845"
                     /inference="COORDINATES: protein motif:HMM:NF015177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX8109601.1"
                     /translation="FFANRPQLARSISVALDVDHQHRLLAIPSRERLERVLRYMLDER
                     EFLSPYGVRSLSAIHAGRPFRLYHDHSYHEVRYAPGESDSGLFGGNSNWRGPVWFPVN
                     YLIIEALKRYHHFYGDALTVECPTGSGRRMNLGEVAEELERRLVSLVLPDARGHRPCH
                     GGAERYARDPAFRDLVLFYEYFHGDTGRGLGASHQTGWTALAANLIERVAATRGGR"
     gene            649..2523
                     /locus_tag="VF516_17850"
     CDS             649..2523
                     /locus_tag="VF516_17850"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX8109602.1"
                     /translation="MTEWLEPDGLGGFAMGTADGIRTRRYHAVLLAATRPPDGRMVLV
                     ADLEVFADTPSGRFALSSHRYQGDVIYPDGAHRLASFPHTPWPRWEWVLPGGARVAGE
                     LVVEHAPAPGRAPRVALRWTRLAGSITSLNVQPLLAGRDYHATHRENPVFRFVPELDG
                     DAVTWRPYTGVPAICCSANGLYHHAPDWYRAFHLRDEAERGLDCIEDLATPGAFTFDL
                     ASGPAAMVLSTGSIAGDPAAVADQIFDAEARRRAAFSSALARAADAYLVARSAGKTVI
                     AGYPWFADWGRDTFIALRGLCLATGRRDDARAILCQWAAAVSRGMLPNRFDEHNTTPE
                     YNSVDTALWFAIAADAYLAGPATAADRYLLRAAIAAIVDGYGAGTRHGVRVDGDGLLA
                     CGQAGVQLTWMDARVDGACITARIGKPVEIQALWINALVIAGRAAEADRARAGFARFW
                     DPGRRQLHDVLDADHVRGAIDSTCRPNQLLAIGGVPHPVLDPQREPARAVVDTVERVL
                     WTPAGPRSLDPGDPRYQGRYAGGAAERDRGYHNGAVWPWLAGPFIEAWVRVRGSTPAA
                     RREARTRFLDPLLARTQLAGLDHLSEICDGDPPHRPTGCPFQAWSLAEAIRIDQLLGA
                     "
     gene            complement(2547..4568)
                     /locus_tag="VF516_17855"
     CDS             complement(2547..4568)
                     /locus_tag="VF516_17855"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8109603.1"
                     /translation="MRGHDRLIDVAFDAMVEIDERGVVTGFNAAAEHTFGYRKADAIG
                     RLLEELIVPERLRELYRASLAQLVATGDLSALDPRIQLTAAHADGTELPVELTLVADE
                     RSDGRRCLLGWLRDLRPHKQMEESLALRAHALEQAQFGIVVSDPVTKQITNINPAYAA
                     LTGYSSEELIGAAGERLFAPGTLEAMAVVADSLRQRGHLTYRARLQRKDGGTVPVIAA
                     SSTAALPSGSTVRISTVIDISEQERAEQARAATQRELERTAARLEILTTTSHEFASAP
                     GDVDAILSLVTRRLVEIIGEGCAVRLISRDGAWVEPSTHVHHPEPAVRELARQLVGSE
                     RQRIGDGLVGTVAASGTALLIPAVDVDEIAARTAPALRPLLASFGVSSVMVLPMRSRG
                     RTLGVISLVRTQGASAYSPEDQRLAQDLVDRAGLAVDNALLVATLEHRVAGRTAALEA
                     ANRDLEAFSYSVSHDLRTPLRAIDGFSQALLEDYQPQLDDTAQRYLQRIREGAQRMGE
                     LIDDLLALARVSRVSLNLVALDLSALARDIVAELRRRDPARATPVHIAPGLSARADAR
                     LLRIALENLLGNAWKYTARHPAAQIWVGAEDSTFHVRDTGAGFDMTYAGKLFEPFQRL
                     HGAKEYEGTGIGLAIVARIISHHGGRIWATGEIDKGATFFFTLGLAARP"
     gene            complement(4937..5317)
                     /locus_tag="VF516_17860"
     CDS             complement(4937..5317)
                     /locus_tag="VF516_17860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109604.1"
                     /translation="MRLVLDNGALIALERNDPAMWRRLKLALATDNVPLSHGGVVGQA
                     WRGRGPRQARLAMALEAIEIRPLDRALGRAAGELLARAGRSDVVDAALVLLASDGDLI
                     VTSDPGDIEPLARAARRNVDLLRV"
     gene            complement(5314..5601)
                     /locus_tag="VF516_17865"
     CDS             complement(5314..5601)
                     /locus_tag="VF516_17865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109605.1"
                     /translation="MTVTVDRDLLDAANEAVASGRASSLSTWVNAAMAERAAKDRYLR
                     ALAAAIADYEAEFGTISEAELIAQQREDRRTAIVVRDRRKRTVRAPRRRIA"
BASE COUNT          802 a         2345 c         2170 g          871 t
ORIGIN      
        1 gttcttcgcc aaccggccgc agctcgcgcg ctcgatctcg gtcgcgctcg acgtcgatca
       61 ccagcaccgc ctgctcgcga tcccgagccg cgagcggctc gagcgcgtgc tgcgctacat
      121 gctcgacgag cgcgagttcc tgtcgccgta cggcgtccgc tcgctgtcgg cgatccacgc
      181 cggccggccg ttccgcctgt accacgatca cagctaccac gaggtccgct acgcgcccgg
      241 cgagtccgac tccggcctgt tcggcggcaa ctccaactgg cgcggcccgg tgtggttccc
      301 ggtcaactac ctcatcatcg aggcgctcaa gcgctaccac cacttctacg gcgacgcgct
      361 caccgtcgag tgcccgaccg gcagcggccg ccgcatgaac ctgggcgagg tcgccgagga
      421 gctcgagcgc cggctggtct cgctggtctt gcccgacgcg cgcggccacc ggccgtgcca
      481 cggcggcgcc gagcgctatg cccgcgatcc ggcgttccgc gatctcgtgc tgttctacga
      541 gtacttccac ggcgacaccg gccgcggcct cggcgccagc caccagaccg ggtggaccgc
      601 gctcgccgcc aacctgatcg agcgcgtcgc cgcgacccgg ggcggccgat gaccgagtgg
      661 ctcgagcccg acggcctcgg cggcttcgcg atgggcaccg ccgacggcat ccgcacccgg
      721 cgctaccacg ccgtgctgct cgccgcgacc cgcccgcccg acggccgcat ggtgctggtc
      781 gccgacctcg aggtgttcgc cgacaccccc tccggccggt tcgcgctgtc gtcgcaccgc
      841 taccagggcg acgtgatcta ccccgacggc gcgcatcgcc tggcgagctt cccgcacacg
      901 ccgtggccgc gctgggagtg ggtgctgccc ggcggtgcgc gcgtcgccgg cgagctcgtc
      961 gtcgagcacg ccccggctcc cggccgtgcc ccgcgcgtcg cgctgcgctg gacccggctc
     1021 gccggcagca tcaccagcct caacgtccag ccgctgctcg ccggccgcga ttaccacgcg
     1081 acccaccgcg agaacccggt gttccggttc gtccccgagc tcgacggcga cgcggtgacc
     1141 tggcggccct acaccggcgt gccggcgatc tgctgctccg ccaacggcct ctaccaccac
     1201 gcccccgact ggtaccgcgc gttccacctc cgcgacgagg ccgagcgcgg gctcgactgc
     1261 atcgaggatc tcgcgacccc cggcgcgttc accttcgacc tcgcgagcgg cccggccgcc
     1321 atggtcctgt cgaccggctc gatcgccggc gacccggccg ccgtcgccga ccagatcttc
     1381 gatgccgagg cccgccgccg cgccgcgttc tcctcggcgc tggcccgcgc cgccgatgcc
     1441 tacctggtcg cgcgcagcgc gggcaagacc gtcatcgccg gctatccgtg gttcgccgac
     1501 tggggccgcg acaccttcat cgcgctgcgc ggcctgtgcc tcgcgaccgg ccgccgcgac
     1561 gacgcgcgcg cgatcctgtg ccagtgggcc gccgccgtgt cgcgcggcat gctgcccaac
     1621 aggttcgacg agcacaacac cacccccgag tacaactcgg tcgacaccgc gctgtggttc
     1681 gcgatcgccg ccgacgccta cctcgccggt cccgccaccg ccgccgaccg ctacctgctg
     1741 cgcgcggcga tcgccgcgat cgtcgacggc tacggcgccg gcacccgcca cggcgtccgc
     1801 gtcgacggcg atggcctgct cgcctgcggc caggccggcg tccagctgac ctggatggac
     1861 gcccgggtcg acggcgcatg catcaccgcg cggatcggca agccggtcga gatccaggcg
     1921 ctgtggatca acgccctcgt catcgccggc cgcgccgccg aggccgaccg cgcccgcgcc
     1981 gggttcgccc ggttctggga ccccggccgc cgccagctcc acgacgtcct cgacgccgac
     2041 cacgtccgcg gcgccatcga ctcgacctgc cgccccaacc agctgctcgc gatcggcggc
     2101 gttccccacc ccgtgctcga tccccagcgc gagcccgccc gcgccgtggt cgacaccgtc
     2161 gagcgcgtgc tgtggacccc ggccggcccg cgctcgctcg accccggcga cccgcgctac
     2221 cagggtcgct acgccggcgg cgccgccgag cgcgatcgcg gctaccacaa cggcgcggtg
     2281 tggccctggc tcgccggccc gttcatcgag gcctgggtcc gcgtccgcgg cagcaccccc
     2341 gccgctcgcc gcgaggcccg cacccgcttc ctcgatccgc tgctcgcccg cacccagctc
     2401 gccggcctcg accacctgtc cgagatctgc gacggcgacc cgccgcaccg ccccaccggc
     2461 tgcccgttcc aggcctggtc gctcgccgag gcgatccgga tcgaccagct gctcggagcg
     2521 tgacccgcgt cgacatccgc cgcgcatcac ggccgcgcgg cgaggccgag cgtgaagaag
     2581 aaggtcgctc ccttgtcgat ctcgccggtc gcccagatcc gtccgccgtg gtggctgatg
     2641 atgcgcgcga cgatcgccag cccgatcccg gtgccctcgt actccttggc gccgtgcaag
     2701 cgctggaatg gctcgaacag cttgccggca tacgtcatgt cgaacccggc cccggtgtcg
     2761 cggacgtgaa acgtgctgtc ctcggcgccc acccagatct gtgcggcggg gtgccgggcg
     2821 gtgtacttcc atgcgttgcc gagcaggttc tccagcgcga tgcgcagcag ccgcgcatcc
     2881 gcgcgggcgg acagtcccgg cgcgatatgg accggggtgg cgcgcgccgg atcgcgtctg
     2941 cggagctcgg ccacgatgtc gcgcgcgagc gccgacaggt cgagcgcgac gaggttgagc
     3001 gagacgcggg acacccgcgc cagcgcgagc aggtcgtcga tcagctcccc catgcgctgc
     3061 gcgccttcgc ggatccgctg caggtaacgc tgggcggtgt catcgagctg aggctggtag
     3121 tcctccagca gcgcctggct gaagccgtcg atcgcgcgca gcggcgttcg caggtcgtgg
     3181 gacaccgagt agctgaacgc ctccaggtcg cgattggcgg cctcgagcgc ggccgtgcgc
     3241 ccggcgacgc gatgctccag cgtcgcgacc agcaaggcgt tgtcgaccgc caggccggcg
     3301 cgatccacga gatcctgagc cagccgctgg tcctcggggg agtaggcgct cgcgccctgg
     3361 gtccggacca ggctgatcac gccgagcgtg cggccccgcg agcgcatcgg gaggaccatg
     3421 acgctcgaga cgccgaagct ggccagcagc ggtcggagcg ccggcgccgt gcgagccgcg
     3481 atctcgtcga catcgaccgc cgggatgagc aacgccgtcc cgctggcagc gacggtgccg
     3541 accaggccgt cgccgatgcg ctggcgctcg ctgcccacga gctggcgagc gagctcgcgg
     3601 accgccggct cgggatggtg cacgtgcgtg ctcggctcga cccacgcccc gtcgcggctg
     3661 atcagccgga cggcgcagcc ctcgccgatg atctcgacca gccggcgcgt caccagcgac
     3721 aggatcgcgt cgacgtcgcc cggcgccgag gcaaactcgt gcgaggtcgt ggtcaggatc
     3781 tccaggcgcg ccgccgtgcg ctcgagctcg cgctgcgtgg ccgcgcgggc ctgctcggcg
     3841 cgttcctgct cggagatgtc gatcacggtg gagatccgga cggtcgagcc cgacggcagc
     3901 gcggccgtgc tcgatgcggc gatcacgggg accgttccgc cgtccttgcg ctgcaggcgg
     3961 gcccggtagg tgagatggcc gcgctggcgg agcgaatccg cgaccaccgc catggcctcc
     4021 agcgtgcccg gcgcgaacaa tcgctcgccg gcggcgccga tcagctcctc gctgctgtac
     4081 ccggtcaagg ccgcatacgc ggggttgatg ttggtgatct gcttggtcac cggatccgac
     4141 acgacgatgc cgaactgcgc ctgctcgagg gcatgcgccc gcaacgccag cgactcctcc
     4201 atctgcttgt gcggccgcag gtcccgcaac cagccgagca agcagcgcct gccgtcgctg
     4261 cgctcgtcgg cgaccagcgt cagctccacc gggagctcgg tcccgtcggc gtgcgccgcg
     4321 gtgagctgga tgcgcgggtc gagcgcggag agatcgccgg tcgcgaccag ctgcgccagg
     4381 ctggcgcgat acagctcgcg aagccgttcg gggacgatca gctcctccag caggcggccg
     4441 atcgcatcgg ccttgcggta accgaacgtg tgctccgcgg ccgcgttgaa cccggtgacc
     4501 acgccgcgct cgtcgatctc gaccatggcg tcgaacgcca cgtcgatcag ccggtcatgg
     4561 cctcgcatga cctctgcact gctcgagctc cgcgctgctg ggacgcattc gcgagtgggc
     4621 aacaggtgat cgcgcggaag gtagcgcgtc gccaagcggc gcgccagata ctccggagtt
     4681 tgcgtggatc gagacgaacg cgcgcgccgg ccgccgcccg tcgcggcgac ggcggcgggc
     4741 tcgcttcact tcgccttgca gtcgcggcgc ttgagctcga cgcggcggtt cctggcctgg
     4801 ttggccggcg agtcgttggg ccggccggga cgccggacct cggccgtggg gggcgatctg
     4861 cctcggcctg cgcgcggtca gttcggcgca gcggcaggag gcggagccgg gcgaggcgca
     4921 ggtgcttgga gcggactcag acgcggagca ggtcgacgtt gcggcgggcg gcccgcgcaa
     4981 gcggctcgat atcaccggga tcggacgtga cgatgagatc gccatcgctg gcgagcagaa
     5041 ccaaggcggc atcgaccacg tcgctgcggc cggctcgcgc cagtaactcg ccggccgcgc
     5101 ggccgagcgc gcgatcgagt ggacggatct cgatggcttc cagtgccatg gccaaccgcg
     5161 cctgacgtgg gccacggccc cgccatgcct ggccgaccac gccgccgtgc gatagcggga
     5221 cattgtcggt cgccagtgca agcttgagcc gtcgccacat cgcggggtcg ttgcgctcca
     5281 gcgcgatcag cgcgccgttg tcgaggacga gcctcatgcg atccggcgtc ggggggctcg
     5341 cacggtcctc ttccggcgat ctcggacgac aatcgccgtg cggcggtcct ctcgctgctg
     5401 ggcgatcagc tccgcctcgg agatcgtgcc gaactcggcc tcgtaatctg cgatcgccgc
     5461 cgcgagcgca cggaggtatc ggtccttggc ggcgcgttcg gccatcgccg cattgaccca
     5521 ggtgctgagc gacgacgctc gcccggaggc cacggcctcg ttggccgcgt ccagcaggtc
     5581 acggtcgacc gtcaccgtca cgcgttcttt gcgtgcgctc atacaaacat cataccatca
     5641 taggacgact tcgatcggga tcgtcctcgt tccgggacct tcagcgcggg ggtgcagcag
     5701 gcggggcggc tggcaagggt gtcggcggag cgccatgtgg tgacgcgggg gcgggcggtg
     5761 cgggggtgcc agtggcggcc ggggtcatcg cggccggtgg ccagatcggc tgcaggccgg
     5821 ggatccgggt ctgcacgcgc gccagcgcct cggcctggcg tacatccaca ccggccacaa
     5881 gctcgagacc agcgtggcga tgcacttctg gtacgacttc ctgctgtcga cggtggcgtt
     5941 cgccgccgat ccgcagaacc agccgttcgt cgtgcagtac agcacgacga tgtgagcggc
     6001 gtccgggacg tcgatcctgc gcaacgatcc ctgcacggcg tccgggcaac cgggtagtct
     6061 gggtgagagg gaatgtccgg gcccggagca acgacggagc cagcgccgcg ggtgcgcatc
     6121 gcgcctgcgg cggcgggcga tggggcgccc cgcggtccgg agccggaggc gcgcggggcc
     6181 ggcccgta
//
LOCUS       DASVOR010000922         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_476952,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000922 DASVOR010000000
VERSION     DASVOR010000922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_476952"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..1842
                     /locus_tag="VF516_17870"
     CDS             433..1842
                     /locus_tag="VF516_17870"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8109606.1"
                     /translation="MCWPDIDSFAREVALPPGVRVRQLARADIPPLVQLLAEWHPRFA
                     AMEDASLLEERFYTENVALAGERHTTMERPVYVALLESEGALVGCLVCDVEPDGVVHY
                     GRFSVVAPHFRRRRLGRANLNLMIALARVVGASVTYDLVELDYLAKQAALEEAGMSLS
                     GVLPNSEWKQVGSEGVRCVPEALYFKTFVPADELIWPEPAALRPATAALMKLLFDYGD
                     PPGPLVSFRPRPVPDLDDAARALLETRRGGDLEWPDARPLAGRLALPPGVAVRRLARA
                     DLPGLIDRLPAWHPDIAHGSQRHLLSPAFYEARVALEGEDAHIERRPAHAWVVERGGE
                     LAGLSVVRCHLEHATLFAEFVVVDPRHRGAGLARVLLHTVMLAAHTIAVDMITAYATL
                     RHPYGQRLCEGAGFRLVGILPAANRHTVSPGVVKHAFEAVYSISLLSPEQSYTPPREA
                     MPPRVPALADFVYGPPEEA"
BASE COUNT          353 a          800 c          804 g          418 t
ORIGIN      
        1 agggctcctt gagcttgagc aggagggtgc ggccaggccc gaccatctcc gtgtgcagat
       61 gaggccagcg ggtcatctcg ccttgtcctc cgcgccctct cgacctccca ggcttccatt
      121 gatcgaggtg atcgtcttgg ccatggtccg agctttaagg atgagcgagg cggcctgagc
      181 gagttacact tcgcggacgc gaagaacgat gggcttgaca aagatcacac gccgtacgta
      241 ggctggtaac tgcatcccca gtccctccct ggttcgacac acctcgttcg ctcgcctggg
      301 cctgcgtaaa ggcgcaatat aacaaggaag gttgaacttg ggatccgtgg cgctctacac
      361 aaggcacctc gacgatctgc gcagcgcgct tcgtcacagc gcagcgcacg aggagggcag
      421 gaagccgtga gcatgtgctg gcctgacatc gactccttcg ctcgcgaggt cgcgctgccg
      481 cccggcgtgc gggtgcggca gctcgcccgc gcggatatcc ctcccctggt ccagctgctc
      541 gcggagtggc accccaggtt cgccgccatg gaggacgcct cgctgctcga ggagcgcttt
      601 tacacggaga acgtggccct ggccggagag aggcacacga ccatggaacg ccccgtgtac
      661 gtggccctgc tggagagcga gggtgcgctc gtgggctgcc tcgtgtgcga tgtcgagccg
      721 gacggggtag tccactacgg ccgcttctcc gtcgtggccc cgcactttcg cagacgcagg
      781 ctcgggcgcg cgaacctgaa tctgatgatc gcgctcgcgc gagtggtcgg cgcgagcgtg
      841 acgtatgacc tcgtcgagct cgactacctg gccaagcagg ccgcgttgga ggaggcaggg
      901 atgtccctga gcggcgtcct gccgaactcg gagtggaagc aggtcggctc ggagggagta
      961 cggtgtgttc cggaggccct ctatttcaag acattcgtcc ccgccgatga gctcatctgg
     1021 ccggagccag cggcgctgcg ccccgcgacc gccgccctga tgaagctcct gttcgattac
     1081 ggcgatccgc ccggccctct cgtgtcgttc cggccgcggc ccgtgcctga tctcgatgat
     1141 gcagcccggg cgctccttga gactcgccgc ggcggagacc tcgagtggcc cgatgcccgc
     1201 cccctcgctg ggcggctcgc cctccccccc ggcgtggccg tgcggagact cgctcgggcc
     1261 gatctcccgg ggctcatcga ccgacttccc gcgtggcatc cggacatcgc gcacggctcg
     1321 cagcgtcacc tgctctcgcc tgccttctac gaggcccgcg tcgcgctcga aggcgaggac
     1381 gcgcacatcg agcgtcgccc ggcgcacgcc tgggtcgtgg agcgcggcgg cgagctcgcc
     1441 ggactgtcgg tcgtgcgctg ccatctggag catgccacgc tgttcgcgga gttcgtcgtc
     1501 gtcgatccgc ggcatcgtgg tgcgggtctt gcgcgcgtcc tgttgcacac cgtcatgctc
     1561 gccgcccata ccatcgcggt ggacatgatc accgcctatg ccacgctccg ccacccttat
     1621 ggccagcgtc tctgcgaggg cgcgggcttc cggctcgtcg gcatcttgcc cgcggcgaat
     1681 cgacacacgg tctccccggg ggtcgtgaag cacgccttcg aggcggtcta ttcgatctcg
     1741 ctgctgtcgc ccgagcagag ctacacgccc ccgcgcgagg cgatgccgcc gcgcgtgccc
     1801 gccctcgctg acttcgtcta cgggcccccc gaggaggcgt aggtatgacc gcgctcagcg
     1861 cataagcgcg agctgtaaga ccctcgtgat tgacgagcgc aggtgtggaa cacgcgcgcc
     1921 gctgacgcac ggggggcacc atggggactc cttccagaat ggttccgcgc tgatttcagc
     1981 tgccagtgca gcggaagccg agaccaagtg cgagaccctg tttggagaaa cggcgttcga
     2041 tgcatctatc tgggacggcc tggaagggtt ctgggtatgt tttcctgcga tcggggggtg
     2101 aggtcgaccc tggggtgagg cgtgaggcgc aggtcgccga gcaagggcac aagctcgacg
     2161 agatcctgca gctgttgacg gagatggcgc tgctcgtgcg atcgcagtga cccaacacga
     2221 ttcacacgtg ggccgatccg cgcgtgctcc ggctcgtcag ccgggccgcg gcagcgggcc
     2281 tggcggtcgc ggcagcggga gcgcctcgct gtcggtcacg atctccgtga tcgacgaggc
     2341 cccgccgccg ttcgagatgc tgctcgacac catca
//
LOCUS       DASVOR010000923         3376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_184798,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000923 DASVOR010000000
VERSION     DASVOR010000923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3376
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184798"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="VF516_17875"
     CDS             <1..186
                     /locus_tag="VF516_17875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109607.1"
                     /translation="ELMTSEQGKNKKPLPPLTVVSHPMSVLAGAIGAGILGAYRFEQL
                     ERQGRIVDAAASVAQTG"
     gene            complement(196..1191)
                     /locus_tag="VF516_17880"
     CDS             complement(196..1191)
                     /locus_tag="VF516_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109608.1"
                     /translation="MSRLRLTLAVGDSDRTRPLFDGTVVPEAIDLVCLRLPVEDIFFR
                     MARFAEFDAAEMSLSSYLISMTETGPGRFVAIPAFLSRSFRHGGIYVNQSSGIVNPED
                     LVGRTVGLAEYQLTANVWIRGLLAEHHGVPVASVRYRTGGLHTPGRGEKMRIQPPSDI
                     DIAPIPRDRTLSDMLAIGELDAVYSPRTPRCFDAGSTAVRRLFSDVRTVEADYYRRTG
                     IFPIMHVVVLRRDVYEANRWAARSLYRALVAARDEAWRGMDETAALRFMLPWLVAEVE
                     TTRAILGNDPWSYGLAGNEKALATLIGYSHAQGLARRAFAPAELFAPETLEDTIV"
     gene            complement(1188..1982)
                     /locus_tag="VF516_17885"
     CDS             complement(1188..1982)
                     /locus_tag="VF516_17885"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8109609.1"
                     /translation="MTRLLEVRRLRKIYEGHGRIVEAIGDLSFACAPGELVCIVGPSG
                     AGKTTLLKCVAGLIAPTTGEVVLGDAPVAGPPPGMAVVFQEYGRSLFPWLTVWRNVAL
                     PLEHGASRDELRRLVDDALAEVGLADVPAAYPWQLSGGMQQRVAIARAIAYQPKILLM
                     DEPFAAVDAQTRADLEDLTLRVWRRHGITLLFVTHDIDEAVYMGQRVLILSASPTRVI
                     EDLAIDLAAERDQLATRSSPRFAELRGRVYEQIQRAKRAGAGEVRP"
     gene            complement(1979..2755)
                     /locus_tag="VF516_17890"
     CDS             complement(1979..2755)
                     /locus_tag="VF516_17890"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8109610.1"
                     /translation="MIRALRVLLAALGLPAALFALWWLVSADSKSFYFPPLRAILGGF
                     GATWSSRWTSDVLPSVGRLLLGYGGAVIAGIGLGVPLGARPRLRAALEPALELFRAIP
                     PPALVPVVMLLFGIGTPMKLLVIASGAVWPVLLNTIEGVRAVDEVLSDTCRSYGITGM
                     RRLRVLVLRGASPQIVTGMRQALSIAIILMVISEMFASSSGLGYAIVDFQRAFSIPQM
                     WSGILLLGVLGFVLSLGFGAFEARALRWYHGQRRAHREDG"
     gene            complement(2752..>3376)
                     /locus_tag="VF516_17895"
     CDS             complement(2752..>3376)
                     /locus_tag="VF516_17895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8109611.1"
                     /translation="AAFWRALLHTIEGWALGLAIAVAAGTVAGVAIGSRRGLRATTAS
                     TIEFLRPIPSVALIPVAVLLYGAQLRSVLLLVVYASFWPVLLQVIHGVADVDPVARDT
                     ALSYRLPRWTQIRTLVLPTALPYLLTGVRLSAAVALILAITAELVIGAPGLGNEIGVA
                     MASSAVPVMYALIFVVGLIGVAVNVGFRALERRVLAWHPSVRREAAA"
BASE COUNT          547 a         1269 c         1172 g          388 t
ORIGIN      
        1 gagctgatga cgagcgagca gggcaagaac aagaagccgc tgcccccgct gaccgtcgtc
       61 tcgcatccga tgtcggtgct cgccggcgcg atcggcgcgg gtatcctggg cgcgtatcgc
      121 ttcgagcaac tcgagcggca agggcgcatc gtcgacgccg ctgcatccgt tgcgcagacg
      181 ggttagcgtt cgccactaca cgatcgtgtc ttccagcgtc tcgggcgcga acagctcggc
      241 cggcgcgaac gcgcggcgcg ccaggccctg ggcgtgcgag tagccgatca gcgtcgcgag
      301 tgccttctcg ttgccggcca ggccatagct ccagggatcg ttgccgagga tcgcgcgcgt
      361 ggtctcgacc tcggcgacca gccacggcag catgaagcgc agcgccgcgg tctcgtccat
      421 cccgcgccac gcctcgtcgc gcgcggcgac aagcgcgcgg tacagcgagc gcgccgccca
      481 gcggttcgcc tcgtacacgt cgcgccgcag cacgacgacg tgcatgatcg ggaagatccc
      541 ggtgcggcgg tagtagtcgg cctcgacggt gcggacgtcg gagaacagcc ggcgcaccgc
      601 ggtggacccg gcgtcgaagc agcgcggcgt gcgcggcgag tacaccgcgt cgagctcgcc
      661 gatcgcgagc atgtccgaca gcgtgcggtc gcgcgggatc ggcgcgatgt cgatgtcgga
      721 cggcggctgg atccgcatct tctcgccgcg gcccggcgtg tgcagcccgc cggtgcgata
      781 gcgcaccgag gccaccggca cgccgtggtg ctcggcgagc aggccgcgga tccagacgtt
      841 ggcggtgagc tggtactcgg cgagcccgac ggtgcgcccg accaggtcct cggggttgac
      901 gatgccgctc gactggttga cgtagatccc cccgtggcgg aacgagcgcg acaggaacgc
      961 cgggatcgcg acgaaccgcc ccgggccggt ctcggtcatg ctgatcaggt agctcgacag
     1021 cgacatctcg gcggcgtcga actcggcgaa ccgcgccatg cggaagaaga tgtcctcgac
     1081 cgggagccgc aggcacacca ggtcgatggc ctcggggacg accgtcccgt cgaacagcgg
     1141 gcgggtgcgg tcggagtcgc cgaccgccag ggtcaagcgc agccggctca cggccggacc
     1201 tccccggcgc ccgcgcgctt ggcccgctgg atctgctcgt agacccgacc gcgcagctcg
     1261 gcgaacctcg gcgacgaccg ggtcgcgagc tggtcgcgct cggccgccag atcgatcgcg
     1321 aggtcctcga tcactcgcgt gggggatgcc gacaggatca acacgcgctg tcccatgtag
     1381 accgcctcat cgatgtcgtg ggtgacaaac aggagcgtga tgccgtgccg gcgccacacc
     1441 cgcaaagtca ggtcctcgag gtcggcgcgg gtctgcgcgt cgaccgcggc gaacggctcg
     1501 tccatcagca ggatcttcgg ctggtaggcg atcgcgcggg cgatcgcgac ccgctgctgc
     1561 atcccgcccg acagctgcca ggggtacgcg gcgggcacgt cggcgagccc gacctcggcg
     1621 agcgcgtcgt cgaccagccg gcgcagctcg tcgcgcgacg cgccgtgctc gagcggcagc
     1681 gcgacgttgc gccacaccgt cagccacggg aacaggctgc ggccgtactc ctggaacacc
     1741 accgccatcc ccggcggcgg accggcgacc ggcgcgtcac cgagcacgac ctcgccggtg
     1801 gtcggcgcga tcagcccggc gacgcacttg agcagcgtgg tcttgccggc gcccgacggc
     1861 ccgacgatgc acaccagctc gcccggcgcg cacgcgaagc tcagatcgcc gatcgcctcg
     1921 acgatccggc cgtggccctc gtagatcttg cgcagccggc ggacctcgag cagccgggtc
     1981 atccgtcctc ccgatgggcc cggcgctggc cgtgatacca gcgcagcgcg cgggcctcga
     2041 acgcgccgaa cccgagcgac agcacgaacc cgagcacgcc gagcaacagg ataccgctcc
     2101 acatctgcgg gatcgagaac gcgcgctgga agtcgacgat cgcgtagccc agcccgctcg
     2161 agctggcgaa catctcgctg atcaccatca ggatgatcgc gatcgacagc gcctggcgca
     2221 tcccggtcac gatctgcggg ctcgcgccgc gcaggaccag cacgcgcagc cggcgcatcc
     2281 cggtgatccc gtagctccgg caggtgtcgg acagcacctc gtcgacggcg cgcacgccct
     2341 cgatcgtgtt gagcagcacc ggccacaccg cgcccgacgc gatcaccagc agcttcatcg
     2401 gcgtgccgat cccgaacagc agcatgacca ccggcaccag cgccggcggc gggatcgcgc
     2461 ggaacagctc cagcgccggc tcgagcgccg cccgcagccg cggccgcgcg ccgagcggca
     2521 cgccgagccc gatcccggcg atcactgccc cgccatagcc gagcaacagg cgcccgacgc
     2581 tgggcagcac gtcgctggtc cagcgcgacg accacgtcgc gccgaacccg ccgaggatcg
     2641 cgcgcagcgg cggaaagtag aagctcttgc tgtcggccga gaccagccac cacagcgcga
     2701 acagcgccgc cggcaacccg agcgccgcga gcagcacgcg cagcgcgcgg atcacgccgc
     2761 cgcctcccgc cgcacggagg ggtgccaggc cagcacccgg cgctcgagcg cgcggaaccc
     2821 gacgttgacc gcgaccccga tcagcccgac cacgaagatc agcgcgtaca tcaccgggac
     2881 cgcgctcgac gccatcgcca cgccgatctc gttgccgagc cccggcgcgc cgatcaccag
     2941 ctcggccgtg atcgccagga tcagcgccac cgccgccgac agccggaccc cggtcaagag
     3001 gtacggcagc gcggtcggca ggaccagggt gcggatctgc gtccaccgcg gcaggcggta
     3061 cgacagcgcg gtgtcgcgcg ccaccggatc gacgtcggcg accccgtgga tcacctggag
     3121 cagcaccggc cagaacgacg cgtagaccac cagcagcagg accgagcgca gctgcgcgcc
     3181 gtacagcagc accgcgaccg ggatcagcgc gaccgacggg atcggccgca ggaactcgat
     3241 cgtcgacgcg gtcgtcgcgc gcagcccgcg ccggctgccg atcgccaccc cggccaccgt
     3301 gcccgccgcc accgcgatcg ccaggccgag cgcccagccc tcgatcgtgt ggagcagcgc
     3361 ccgccagaac gccgcg
//
LOCUS       DASVOR010000924         5286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417749,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000924 DASVOR010000000
VERSION     DASVOR010000924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417749"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1466
                     /locus_tag="VF516_17900"
     CDS             <1..1466
                     /locus_tag="VF516_17900"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8109612.1"
                     /translation="CRHLAAGVSLVLATATAAADPTIASSTTPPARPDSDVVAPRRGP
                     LEHELTAADVRTAPLPGQEGGQLQPDPGDSTLRVIGRGILFLPKLAIEVVLSPVRGAI
                     WADDRYRLEDVYHRVFYSADRTIGLFPTGTYTSGFGFAAGAGFVDSQLFGKNESLALE
                     AMTGAITGDTYRVNALGSLRSGQRFSRWLQVGIDATFERRPADPFYGIGNGELVAPTG
                     SPIRPQTDPTAVETYHRYQEARIAVVGNARVIDAFQVLGTGALTSLRFARSTTGTPID
                     EVYAPEDLVGFTTGVRHAYGELELRWDTRGRATPWEPRDVHSVGSLAAAFAGRVHRLD
                     GGPDFWRYGVELQHNWRLARGPRVVTVRLHGAGVTGERDEVPFTELPTLGGGGFLRGY
                     PYERFRDRVAAFGTIQYQWDISHLVDAYLFSDAGRVFPSPDALTVHGMRLGYGIGLEL
                     HGQNGFLLEGSLASSIDGGVFVSVALNPVLDQKERWR"
     gene            1463..3139
                     /locus_tag="VF516_17905"
     CDS             1463..3139
                     /locus_tag="VF516_17905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109613.1"
                     /translation="MRPVWILCAVLSGCASGPVVPEVRFANAPVAVAVNDRLDVKRPP
                     HTRLFLPDTYVWDGTVGRRISRALELPRDRRALGVNALDEVPDSTWFTNRIGVRELTP
                     DEVRNGPLTDDPERHKPWTIHSTKIGGTALGFIIKDASGVKYLLKFEPADQAPELESG
                     NHVVVNRLLWACGYNVAEDRIVYLRSDDLVLAPDATVKDLAADDQRKLDRAELERDLA
                     MVRHEPDGRLRVLASRWIDGVTIGGHPGEGVREDDPNDRIPHELRRDLRGMYPIYAWV
                     DSVDVTEGQYVDAWVADPAEPSRHYVKHYLIDFGKSLGAMGDIAVDWWRSMYYRVDIT
                     GMLRNLMTFGLTDRPWEHRHAPRVKGVASMFEARTFDPGSWHPDSPGYLPFRTADRFD
                     KFWGAKLVAKFTRDQIRAAVEAGRFSDPRAVDYLTDTLVARQRATAAYWFARVNPLDR
                     FSLEAAAGHDELCFDDLAIAARVATAAATQYTITSYDREGREIEASASAAASDGHACV
                     RIRLATPDARDGYTIVRLATTRPGFTGDTFVHVARDPSTGALRVIGVWRR"
     gene            complement(3157..3378)
                     /locus_tag="VF516_17910"
     CDS             complement(3157..3378)
                     /locus_tag="VF516_17910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109614.1"
                     /translation="MARNADEVVAIQASMRASELRIDRRPETRITTVGRGRVTTRRTG
                     IPPTGAEPGVTYRDVDLSVEIVSRPESTS"
     gene            complement(3380..4342)
                     /locus_tag="VF516_17915"
     CDS             complement(3380..4342)
                     /locus_tag="VF516_17915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109615.1"
                     /translation="MTSSDGRDPHQAGPRPDGDPREAEERVGRSDQQPADRRKQPGDG
                     EKRRADGETQRADADARAEGKAADAQHAPELRGSERIEVPGAELAPAEHAPALDDHER
                     AMQRRDREDPDVLLDIPIVKVDEISLEVEDLSAQVSVAARVGNLVLLNVGAEAHLGRL
                     NLTIKGVEAKALLKVYLENVYAVLARTLATLDRNPELVARLLEPVGQAVSSLGRTVEK
                     TVPQLGQSLGNTVEKTVPEVGRSVGNTVEKTVPEVGRSVGNTVEETVPEVGKAAEGAL
                     KKSRVMQPITRAVHRVAHTTRRARDLFHIRRRGAAMVRQGAPRR"
     gene            4696..5028
                     /locus_tag="VF516_17920"
     CDS             4696..5028
                     /locus_tag="VF516_17920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109616.1"
                     /translation="MLGPTLYATLHFRLRAAERALPPDVEEFLRMWGTEIWAAGARQI
                     TVSLPELPAELRDTMIARRAQDWILVAAPSGALITCYHRRNAWRFITRKSEDRRRRVP
                     RHRRSRRR"
BASE COUNT          724 a         1919 c         1826 g          817 t
ORIGIN      
        1 gctgccggca cctggccgcc ggcgtgtcgc tcgtgctcgc gaccgcgacg gccgccgccg
       61 atcccaccat cgcgtcgtcg accacgccgc ctgcccgccc ggacagcgac gtcgtggcac
      121 cgaggcgagg cccgctggag cacgagctca cggcggccga cgtgcgcacg gcgccgctgc
      181 cgggacagga gggcggccag ctccagccgg accccggcga ctccacgctg cgcgtgatcg
      241 gccgcgggat cctgttcctg cccaagctgg cgatcgaggt cgtgctgtcg ccggtgcgcg
      301 gcgcgatctg ggcggatgac cgctaccggc tggaggacgt ctatcaccgg gtgttctaca
      361 gcgccgatcg gacgatcggc ctgttcccga ccgggacgta caccagcggg ttcgggttcg
      421 ccgcgggcgc cgggttcgtg gatagccagc tgttcggcaa gaacgagagc ctggcgctgg
      481 aggcgatgac cggcgcgatc accggcgaca cctatcgcgt caatgccctc ggctcgctgc
      541 gcagcggcca gcgattctcg cgctggctgc aggtcgggat cgacgcgacc ttcgagcgcc
      601 ggccggccga cccgttctac ggcatcggca acggcgagct ggtcgccccg accggctcgc
      661 cgatccgccc gcagaccgat ccgaccgccg tcgagaccta ccatcgctat caggaagcgc
      721 ggatcgcggt ggtcggcaac gcgcgcgtga tcgacgcctt ccaggtgctc ggcaccggcg
      781 cgctcacctc gctgcggttc gcgcgcagca cgacgggcac gccgatcgac gaggtctacg
      841 cacccgagga cctggtgggc ttcacgaccg gggtccgcca cgcgtacggc gagctcgagc
      901 tgcgctggga cacgcggggg cgagccacgc cgtgggagcc gcgcgacgtt cattcggtcg
      961 gatcgctcgc ggccgcgttc gcgggccggg tgcaccggct cgacggcggc cccgacttct
     1021 ggcgctacgg cgtcgagctc cagcacaact ggcggctcgc ccggggcccg cgcgtggtga
     1081 ccgtgcgctt gcatggcgcg ggcgtgaccg gcgagcgcga cgaggttccg ttcaccgagc
     1141 tgcccacgct gggcggcggc ggcttcctgc gcggataccc gtacgagcgg ttccgcgacc
     1201 gggtcgccgc attcggcacg atccagtacc agtgggacat ctcgcacctg gtcgacgcgt
     1261 acctgttcag cgacgccggg agggtgttcc cgtcgcccga cgcgctcacc gtccacggca
     1321 tgcggctggg ctacggcatc gggctcgagc tgcatggtca gaatggcttc ctgctcgagg
     1381 gcagcctcgc gagctcgatc gacggcggcg tgttcgtgag cgtggcgctc aacccggtgc
     1441 tcgatcagaa ggagcgatgg cgatgaggcc ggtctggatc ctgtgcgccg tgctgtccgg
     1501 gtgcgcgagc ggcccggtgg tccccgaggt ccggttcgcc aacgcgccgg tcgccgtcgc
     1561 ggtcaacgat cggctcgacg tcaagcgacc tccgcacacc cggctgttcc tccccgatac
     1621 ctacgtgtgg gacggcacgg tcggccggcg gatctcgcgc gcgctcgagc tgccgcgcga
     1681 ccgccgcgcg ctgggcgtca acgccctcga cgaggtgccg gactcgacct ggttcaccaa
     1741 ccggatcggc gtgcgcgagc tgacccccga cgaggtccgc aacggcccgc tgaccgacga
     1801 tcccgagcgc cacaagccgt ggacgatcca cagcacgaag atcggcggca ccgcgctcgg
     1861 cttcatcatc aaggacgcga gcggcgtcaa gtacctgctg aagttcgagc cggccgacca
     1921 ggcgcccgag ctcgagagcg gcaaccacgt cgtggtcaac cggctgctgt gggcgtgcgg
     1981 ctacaacgtg gccgaggatc gcatcgtcta cctgcggtcc gacgacctgg tcctggcgcc
     2041 ggacgcgacc gtcaaggacc tggccgcgga tgaccagcgc aagctcgatc gcgcggagct
     2101 cgagcgcgac ctcgcgatgg tccgccacga gcccgacggc cggctccgcg tcctggcgtc
     2161 gcgctggatc gacggcgtca cgatcggcgg ccacccgggc gagggcgtgc gcgaggacga
     2221 tccgaacgat cggatcccgc acgagctgcg ccgcgacctg cgggggatgt acccgatcta
     2281 cgcgtgggtc gactcggtcg acgtgaccga gggccagtac gtcgatgcct gggtcgccga
     2341 cccggcggag ccgagccgcc actacgtgaa gcactacctg atcgacttcg gcaagagcct
     2401 gggcgcgatg ggcgacatcg cggtcgactg gtggcgcagc atgtactacc gggtcgacat
     2461 caccgggatg ctgcggaacc tgatgacctt cgggctgacc gatcggccgt gggagcatcg
     2521 ccacgcgccg cgggtcaagg gcgtggcctc gatgttcgag gcccggacgt tcgatccggg
     2581 ctcctggcac cccgactcgc ccggctatct cccgttccgc accgccgacc ggttcgacaa
     2641 gttctggggc gccaagctgg tcgcgaagtt cacccgcgac cagatccgcg ccgcggtcga
     2701 ggccggtcgg ttctccgatc cgcgcgcggt cgactacctc accgacacgc tggtcgcgcg
     2761 ccagcgggcg accgccgcgt actggttcgc gcgcgtcaac ccgctcgatc gattctcgct
     2821 cgaggcggcg gccggtcacg acgagctgtg cttcgacgac ctcgcgatcg ccgcgcgggt
     2881 cgcgacggcg gcggcgaccc agtacacgat cacgagctac gacagggagg gacgcgagat
     2941 cgaggcgtcg gccagcgccg cggcgagcga tggccacgcg tgcgtacgca tccggctggc
     3001 cacccccgac gcccgcgacg gatacaccat cgtgcgactg gcgaccaccc ggcccggctt
     3061 cacgggcgac acgttcgtgc acgtcgcacg cgatccgagc accggcgcgc tgcgcgtgat
     3121 cggcgtgtgg cggcgatgat caccctcgca gcgagttcag gaagttgact cgggcctgct
     3181 cacgatctcc accgagaggt cgacgtcccg gtaggtcacg ccgggctcgg cgcccgtcgg
     3241 cgggatgccg gtgcgacgcg tggtgacgcg gccgcggccg accgtcgtga tccgggtctc
     3301 gggtcggcga tcgatgcgca gctcgctcgc gcgcatcgag gcctggatcg cgaccacctc
     3361 gtctgcattc cgggccatgt cacctccgcg gggccccctg gcgcaccatg gccgcgcctc
     3421 ggcggcgaat gtggaagaga tcgcgcgcgc gacgcgtcgt atgtgcaacc ctgtgaacgg
     3481 cgcgggtgat cggctgcatc acgcgcgact tcttgagcgc gccctccgcc gccttgccga
     3541 cctcgggcac cgtctcttcc accgtgttcc ccaccgacct gccgacctcg ggcaccgtct
     3601 tctccaccgt gttccccacc gacctgccga cctccggcac cgtcttctcc accgtgttcc
     3661 cgagcgattg accgagctgc ggcacggtct tctcgacggt cctgcccagc gaggagacgg
     3721 cctggccgac cggctcgagc agcctggcca ccagctcggg gtttcgatcg agggtcgcca
     3781 gcgtccgcgc gaggacggcg tacacgttct ccaggtacac cttgagcagc gccttggcct
     3841 cgaccccctt gatggtcagg ttcaagcgcc cgaggtgcgc ctcggcgccg acattcagca
     3901 ggacgagatt tcccactctc gccgccaccg agacctgtgc actcaggtcc tcgacctcca
     3961 gcgagatctc gtcgaccttg acgatcggga tatcgaggag gacatcgggg tcttcccggt
     4021 cacgtcgctg catcgcccgc tcgtggtcat ccagcgccgg tgcgtgctcc gccggtgcca
     4081 gctcggctcc gggaacctcg atgcgctcgc tgccccgtag ctcgggcgca tgctgcgcgt
     4141 ccgcagcctt cccctcggcg cgcgcgtcgg cgtcggctcg ctgcgtttca ccgtcggctc
     4201 gccgcttctc gccatctcct ggttgcttcc tgcggtcggc cggctgctgg tcgctccgcc
     4261 ccacgcgctc ctcggcctcg cgcggatcgc cgtcggggcg aggtcccgct tgatgcgggt
     4321 ctctcccatc gctgctggtc atcgagccct cgttcgatga gcaacgtggg ttgcatcgca
     4381 cgtgccttcg cgtgaccgcg cgtgaaatcc cgaggaggaa tcgccgcccg ctggccacct
     4441 gccggcatca gggcgaacgc ccaactctga cacgactcga ggtgtctcgc accggcgggc
     4501 gaacgcggga cgtcgattgc cgcagattgc agggtcgaga gccggcgaat gccgatgtcg
     4561 cgctccgcgc gcccgcgtcg cctacccgaa catgagggac ctggggagct gggggacgag
     4621 tggtgatcgg gcaaccgatc gcgtgcaatg ccgccgaatc agcgccgggc gtcatccctg
     4681 agatggcgaa tccatatgct cggtccgacg ctttatgcca cgttgcattt tcgcctccgc
     4741 gcggccgaac gtgccctgcc gccggacgtc gaggagttcc tgcggatgtg gggcaccgag
     4801 atctgggcgg ccggcgcgcg acagatcacc gtatccctcc cggagctgcc ggcggagcta
     4861 cgcgacacga tgattgcccg gcgcgcccag gactggatcc tcgtcgcggc ccccagcggc
     4921 gcgctgatca cctgctacca ccgccgcaac gcgtggcgct tcatcacgcg caagtccgag
     4981 gatcgccggc gacgggtgcc gcggcaccgg cggtcgcgac gccgatgaaa cggtgtctcg
     5041 tggtgacttt ccgggctcgt gttccgatcg tgttccgacg tgttccggct gttccggagc
     5101 atgtttcgcg ccctgcacgt gctggctcgc cgtggctcgt ccccccgcgc gaccgttctc
     5161 caggaccggt ctggcgatcg aaccggggaa ggtcctcgac ctcggacggg tcgtgctcga
     5221 tgcgccaccg ccccggtgac tgtgctcagg gcgcgtcctc gaggatcgcg agccacggcc
     5281 cgccgg
//
LOCUS       DASVOR010000925         6638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417751,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000925 DASVOR010000000
VERSION     DASVOR010000925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417751"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VF516_17925"
     CDS             <1..417
                     /locus_tag="VF516_17925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109617.1"
                     /translation="VAPAPPAVPETAPAGAAPLADQPDPRDRRLELAGMIGGGSMLVL
                     GVVLWGAASSTQDDINHAPTRTRQDLANLKDLESRGDVYAAAGNVLVATGVVLGGVAT
                     YLYIRDRRAASASTARLTPTVLPHGAGLVLTLGGMP"
     gene            complement(395..1153)
                     /locus_tag="VF516_17930"
     CDS             complement(395..1153)
                     /locus_tag="VF516_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109618.1"
                     /translation="MQPFAVEVQTSGGMVLMQPCLQVTIAPRHATPTLQVTSHAHADP
                     QLTAPPHDPVPLQLRLQAPVPQVSELPHDPLPVQATAQVPAPQVTAPPHELGPPQVTL
                     QVPRPHMRFLQVWRALHTIVHDVALSQLTPALHWLGTEHATLQAHPVGHVIAWLQAPL
                     LAEQSIVQVFWAALHEVHGDGHTAASPSGGVSTTPESAAITQNPSTQVRPPAQRCWAS
                     QAKSVLRWLIEQPAASATARAVVQSASFTASLRA"
     gene            complement(1178..1396)
                     /locus_tag="VF516_17935"
     CDS             complement(1178..1396)
                     /locus_tag="VF516_17935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109619.1"
                     /translation="MLRTLVRSLSLVAILAGTAVVSAPAEAAPRAQSSKAVHEQARKA
                     KKSARKTQKHQKRKAKLAKKRAKAAPAQ"
     gene            1681..2202
                     /locus_tag="VF516_17940"
     CDS             1681..2202
                     /locus_tag="VF516_17940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109620.1"
                     /translation="MLRAILCAVLVLTACRKQIDPQPEHPQEAPPLPPASGTPVGYLL
                     DNATQLNLREDQLAKLQEIDRSLSARDDEIETQLRLIEKPEADPEVPKGTPPPRHNHA
                     PGAQVKTTPDAAKLREARKSNDDDALRKAFALLDPAQQTTARKLLEDRGVTPPADARP
                     AKRESGDGAPAQP"
     gene            2260..4131
                     /locus_tag="VF516_17945"
     CDS             2260..4131
                     /locus_tag="VF516_17945"
                     /inference="COORDINATES: protein motif:HMM:NF013560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEX8109621.1"
                     /translation="MRKTALLVLPILAAACGNKPKPEPAPTPEPPPEKVEKQPAPSGD
                     AALTAEAKQFVANLDKTIRKLTVDASEAQWANETDLTPAHEAATAKASEVQSVETTRL
                     VKTARKFEPVLDKLDPDTRRQLLLLKFQAQPSPDDPQQAKQLAELGAKMDSEYGKGVC
                     KTEGGKEVCQDIEHWSKLLQNERNPAKLLATWKTWHDEVGHKERDLFVKFVDLANAGA
                     RAIGFKDVASIWKSGYDMPEAEFAATVDKLWGQVKPLYEQLHCYTRRKLNQKYGDKVV
                     AKTGLIPSHLLGNMWAQSWDYLYPELEPYKGVAPIDVTPELAKHYDAKKMVQVGEAFY
                     TSLGMDPLPATFWERSQLTKPADKNVVCHASAWDVQYNNDLRIKMCINLNQEDLWTIH
                     HELGHDYYFHYYYKLPMLYQAGANDGFHEAIGDAIQLSMTPAYLKEKGLLATVVKNDK
                     ATINQQMQVALGKIAFLPFGLLVDKWRWDVFSGEVKPEQYNQHWWDLKKQYQGVAPPV
                     ERAATDFDPGAKYHVAANVPYMRYFLAAVLQFQFHKALCAKAGYKGPLYECSIYGNKD
                     AGAAYMKMLALGASKPWQDALFELTGTRNMDASPILEYFAPLQGWLTEQNKGQTCGW"
     gene            4469..5515
                     /locus_tag="VF516_17950"
     CDS             4469..5515
                     /locus_tag="VF516_17950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109622.1"
                     /translation="MITTTRLALVATLALSATTACGPGSSGMGGDDGGGGGDDQPPTK
                     PLDPTGTYAVHSTFDLATNMPGTAGAVVNTIIDATNDVDDPTRWIVDQILGQLPSGTV
                     KTLLQGAEAIGVGALNDELKKVTPDFVSTMLQVGQDFGDLAKHVGLKETLTLTQSQAA
                     GSYSATHAVVGLHYELGNQKADLLFANYQLPNVQVDTAVTMDATGQLTIAAHDLPLTY
                     GKLLRLGLDAAIIPMIDSSAHNLNDLLAHQIDCKAVGNAIASAIDFSSAAGTLASACS
                     AGLSAGASVVYNKITAIDGTALKFSINGSARAVDRNNDRQIDQIQTGTWAGTLAYGTT
                     PTPLIPATFFGERQ"
     gene            complement(5610..6491)
                     /locus_tag="VF516_17955"
     CDS             complement(5610..6491)
                     /locus_tag="VF516_17955"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8109623.1"
                     /translation="MTFLRRWVAWVETSDAAAAHWLRAAHDALLGEMPVLAAGTALFA
                     ILATVPTLAAVVGVYGLIADPHAIHNHLRGLETVLPPEVVTFVGDQLERQATRSSGEL
                     GIAVATSIAVAVYSARGAARALIAVLNRAYRVRDRRHVVHKILLSVAMAGATLVGLML
                     MFGVVVALPGIVALLHLQGYGLVRWFRWPSLMALLYGALVLLYRYGPSPRPMVERHLW
                     PGAMTATLLLVLVSWGLSQWVERVSHYELFYGTFGSIVVVLLWFYLSTIALVLGGFLN
                     AELERHAGAPAPERTMY"
BASE COUNT         1128 a         2472 c         2180 g          858 t
ORIGIN      
        1 gtcgcgcccg ccccgccggc ggtgcccgag accgcgcccg ccggcgctgc cccgctcgcc
       61 gatcagcccg atccgcgcga tcgccgcctc gagctcgccg gcatgatcgg cggcggcagc
      121 atgctggtgc tcggcgtggt cctgtggggc gccgcgagca gcacccagga cgacatcaac
      181 cacgcgccga cgcggacccg ccaggacctc gccaacctga aggacctcga gtcccggggc
      241 gacgtctacg ccgccgccgg caacgtgctc gtcgccaccg gcgtcgtgct cggcggcgtc
      301 gcgacctacc tctacatccg cgatcgccgc gccgcctcgg catcgaccgc gcgcctcacg
      361 cccaccgtgc tccctcacgg cgccggcctg gtgctcacgc tcggagggat gccgtgaagc
      421 tcgcgctctg caccaccgcc ctggccgtcg cgctcgcggc cggctgctcg atcagccacc
      481 gcagcaccga cttcgcctgc gacgcccagc agcgctgcgc gggcggccgg acctgcgtcg
      541 acgggttctg cgtgatcgcg gccgactccg gcgtggtcga caccccgccg ctcggcgacg
      601 cggcggtgtg cccgtcgccg tgcacctcgt gcaacgccgc ccagaagacc tgcacgatcg
      661 attgctcggc cagcaatggc gcctgcaacc aggcgatcac gtgcccgacc gggtgggcct
      721 gcaacgtggc gtgctcggtc cccaaccagt gcaacgccgg cgtgagctgc gacaacgcca
      781 cgtcgtgcac gatcgtgtgc agcgcccgcc agacctgcaa gaacctcatg tgtggccgcg
      841 gcacctgcaa cgtcacctgc ggcggcccca attcgtgcgg cggcgcggtc acctgcggcg
      901 ccgggacctg cgctgtcgcc tgcaccggca acgggtcgtg cggcaactcg ctcacctgcg
      961 gcaccggcgc ctgcaacctc aactgtagcg gcaccggatc gtgcgggggc gcggtgagct
     1021 gcggatcggc atgcgcgtgc gacgtgacct gcaaggtcgg cgtggcgtgt ctcggcgcga
     1081 tggtcacctg caagcagggc tgcatgagca ccatgccgcc gctggtctgc acctcgacgg
     1141 cgaacggctg caatacctgc ctgtagccgc gagcgggcta ctgcgccggg gcggccttcg
     1201 cgcgcttctt ggcgagcttg gccttccgct tctggtgctt ctgggtcttg cgcgccgact
     1261 tcttggcctt gcgcgcctgc tcgtgcacgg ccttcgagct ctgggccctg ggcgcagcct
     1321 cggccggggc gctcaccacc gcggtaccgg cgaggatggc aacgagggag agcgagcgaa
     1381 ccagcgtgcg cagcatgggg tgacacccta ggtaacgcat tcagtacctc gggacaacat
     1441 cttcgcgtgg cacccacatc ggcgaggctg tcggcgcatc gggagagacc tctcgcggct
     1501 ccccacgctg gacgaccctc cgctctaacg gagtaagtgt ttaaaattca tcggttttat
     1561 ccgaaaaacc cggaaaattt tctcacggaa gcgaggcaac ctctcgcgga cctgcgggtg
     1621 gagctgcgtc ggtgccgcgc acacacgcgc gcacggtcgg gcgggagata gagtgccgcg
     1681 atgctgcgag ccattctttg cgcggtgctg gtcctgaccg cgtgccgcaa acagatcgat
     1741 ccccagccgg agcaccccca ggaggcgccg ccgctgccac cggcgtccgg caccccggtc
     1801 ggctacctgc tcgacaacgc cacccagctc aacctccgcg aggatcagct cgccaagctc
     1861 caggagatcg atcgcagcct gtccgcgcgc gacgacgaga tcgagaccca gctccggctg
     1921 atcgagaagc ccgaagcgga tcccgaggtg cccaagggca caccgccgcc gcgccacaac
     1981 cacgcgccgg gcgcacaggt caagaccacg cccgacgccg ccaagctgcg cgaggcccgc
     2041 aagtcgaacg acgatgatgc gctgcgcaag gcgttcgcgc tgctcgatcc ggcgcagcag
     2101 accaccgcgc gcaagctgct ggaggaccgc ggcgtcacgc cgcccgccga cgccaggccg
     2161 gccaagcgcg agtcgggcga cggcgcgccg gcccagccct gacgtcgcga acgcgcggcg
     2221 agccgtcctc gcggcgagcc gggagggtag agtgcggccg tgcgcaagac cgccctgctc
     2281 gtcctcccga tccttgccgc ggcgtgcggc aacaagccca agccggagcc ggcgcccacg
     2341 cccgagccgc cgcccgagaa ggtcgagaag cagcctgcgc cgagcggcga cgcggcgctg
     2401 accgccgagg ccaagcagtt cgtcgccaac ctcgacaaga ccatccgcaa gctgacggtc
     2461 gacgcctcgg aggcacagtg ggccaacgag accgacctga cccctgcgca cgaggccgcg
     2521 accgccaagg cgagcgaggt ccagagcgtc gagaccacca ggctggtcaa gaccgcgcgc
     2581 aagttcgagc cggtgctcga caagctcgac cccgacaccc ggcgccagct gctgctgctc
     2641 aagttccagg cccagccgtc gcccgacgat ccgcagcagg ccaagcagct cgccgagctc
     2701 ggcgccaaga tggactcgga gtacggcaag ggcgtgtgca agaccgaggg cggcaaggag
     2761 gtctgccagg acatcgagca ctggtccaag ctgctccaga acgagcgcaa ccccgccaag
     2821 ctgctcgcga cctggaagac ctggcacgac gaggtcggcc acaaggagcg cgacctgttc
     2881 gtcaagttcg tcgacctcgc caacgccggc gcgcgcgcga tcgggttcaa ggacgtcgcg
     2941 tcgatctgga agtcgggcta cgacatgccc gaggccgagt tcgccgcgac ggtcgacaag
     3001 ctgtggggcc aggtcaagcc gctctacgag cagctgcact gctacacccg ccgcaagctc
     3061 aaccagaagt acggcgacaa ggtcgtcgcc aagaccggcc tgatcccgtc gcacctgctc
     3121 ggcaacatgt gggcgcagtc gtgggactac ctctaccccg agctcgagcc gtacaagggc
     3181 gtcgcgccga tcgacgtcac gcccgagctc gccaagcact acgacgccaa gaagatggtg
     3241 caggtcggcg aggcgttcta cacctcgctc ggcatggacc cgctgccggc gacgttctgg
     3301 gagcgctcgc agctcaccaa gccggccgac aagaacgtcg tgtgtcacgc cagcgcgtgg
     3361 gacgtccagt acaacaacga cctccggatc aagatgtgta tcaacctgaa ccaggaggac
     3421 ctgtggacga tccaccacga gctcggccac gactactact tccactacta ctacaagctc
     3481 ccgatgctgt atcaggccgg ggccaatgac ggcttccacg aggcgatcgg cgacgcgatc
     3541 cagctgtcga tgacgccggc ctacctcaag gagaagggcc tgctcgccac ggtggtcaag
     3601 aacgacaagg cgacgatcaa ccagcagatg caggtcgcgc tgggcaagat cgcgttcctg
     3661 ccgttcggcc ttctggtcga caagtggcgg tgggacgtgt tctccggcga ggtcaagccg
     3721 gagcagtaca accagcactg gtgggacctg aagaagcagt accagggcgt cgccccgccg
     3781 gtcgagcgcg cggcgaccga cttcgacccc ggcgccaagt accacgtcgc ggccaacgtg
     3841 ccctacatgc ggtacttcct cgccgcggtg ctgcagttcc agttccacaa ggcgctgtgc
     3901 gccaaggccg gctacaaggg cccgctctac gagtgctcga tctacggcaa caaggacgcg
     3961 ggcgcggcgt acatgaagat gctcgcgctc ggcgcgagca agccgtggca ggacgccctg
     4021 ttcgagctca ccggcacccg caacatggac gcctcgccga tcctcgagta cttcgcgccg
     4081 ctgcagggct ggctgaccga gcagaacaag ggccagacct gcggctggtg agccccgcgc
     4141 gcgcccgcac ggcgtagccc gccccgtcgc cgtcgggcgc gctgcccggg cgcgctgtat
     4201 gaacgttggg gcggcttaca tgcgtttcac gccgcccgcc gagcgccgag ccgcgccggc
     4261 gtcgtcggag atcgtggctc ccggcggctc ccgcgcgatc ggcaacccga gtggtcgggc
     4321 cgccagacac ctgcgtcgcg cgaccggctg atcgacttgc cagcgactcg cgacaactgc
     4381 gcgatgccac agcggtcgcg tcgcggtgac ccgtggcaca tgcgctgcac aaggcgctcg
     4441 gcagcaatcc tcgccgggag aaccagccat gatcaccacg actcgcctcg ctctcgttgc
     4501 cacgctcgcc ctgagtgcca ccaccgcctg cggccccggc tcctccggga tgggaggcga
     4561 cgacggcggc ggcggcggcg atgaccagcc gccgaccaag ccgctcgacc cgaccggcac
     4621 gtacgccgtg cacagcacgt tcgacctcgc gaccaacatg cccggcaccg cgggcgccgt
     4681 ggtcaacacg atcatcgacg ccaccaacga cgtcgacgac ccgacgcggt ggatcgtcga
     4741 ccagatcctc ggccagctgc ccagcggcac ggtcaagacc ctgctccagg gcgccgaggc
     4801 gatcggcgtc ggcgcgctca acgacgagct gaagaaggtc acgcccgact tcgtctcgac
     4861 catgctccag gtcggccagg acttcggcga cctcgccaag cacgtcggcc tcaaggaaac
     4921 gctcacgctc acccagtcgc aggcagccgg cagctactcc gcgacgcacg ccgtggtcgg
     4981 cctgcactac gagctcggca accagaaggc cgacctgctg ttcgccaact accagctgcc
     5041 caacgtccag gtcgacaccg ccgtcacgat ggacgcgacc ggccagctga cgatcgccgc
     5101 gcacgacctg ccgctcacct acggcaagct gctgcggctc gggctcgacg ccgcgatcat
     5161 cccgatgatc gactcgtcgg cgcacaacct caacgacctg ctcgcgcacc agatcgactg
     5221 caaggcggtc ggcaacgcga tcgccagcgc gatcgacttc agctcggcgg ccggcacgct
     5281 ggcctcggcg tgcagcgcgg ggctctccgc cggcgccagc gtcgtgtaca acaagatcac
     5341 cgcgatcgac ggcaccgcgc tgaagttctc gatcaacggc agcgcccgcg cggtcgaccg
     5401 caacaacgac cgccagatcg atcagatcca gaccggcacg tgggccggga ccctggcgta
     5461 cggcacgacc ccgacgccgc tgatcccggc cacgttcttc ggcgagcggc agtagctcgc
     5521 acccaacggt tcggcgcggc ggaaggtgag tctgcccgtc gaggatggct ggccagcccg
     5581 ggaaatggtt cccgggctgg ttttttttgt cagtacatcg tgcgctccgg ggccggcgcg
     5641 ccggcgtggc gctcgagctc cgcgttgagg aagccgccga gcaccagcgc gatggtcgac
     5701 aggtagaacc acaagagcac gacgacgatg ctgccgaacg tgccgtagaa cagctcgtag
     5761 tggctgaccc gctcgaccca ctgcgacagc ccccacgaca ccagcaccag cagcagggtc
     5821 gcggtcatcg cgcccggcca caggtggcgc tcgaccatcg gccgcggcga cggcccgtag
     5881 cgatacagga gcaccagcgc cccgtacagc agcgccatca ggctcggcca gcggaaccag
     5941 cgcaccagcc cgtagccctg aaggtggagc agcgcgacga tcccgggcag cgcgaccacg
     6001 acgccgaaca tcagcatcag gccgaccagc gtggcgcccg ccatcgccac cgacagcagg
     6061 atcttgtgca cgacgtggcg gcgatcgcgg acccggtagg cgcggttgag caccgcgatc
     6121 agcgcgcgcg ccgcgccgcg cgccgagtac accgccacgg cgatgctggt cgccaccgcg
     6181 atcccgagct cgccgctcga ccgcgtcgcc tggcgctcga gctggtcgcc gacgaacgtc
     6241 accacctcgg gcggcagcac ggtctccagg ccgcgcaggt ggttgtggat cgcgtgcggg
     6301 tcggcgatca ggccgtacac cccgaccacc gcggccagcg tcggcacggt cgccaggatc
     6361 gcgaacagcg ccgtgccggc cgccagcacc ggcatctcgc cgagcagcgc gtcgtgcgcc
     6421 gcccgcagcc agtgcgcggc cgcggcatcg ctggtctcta cccaggccac ccagcgtcgc
     6481 aggaacgtca cgcctccatg ctaccgtgag cacgcggcgg tgggatcccg cctgactcgc
     6541 cgtgatcgac acgcactgcc acctcgacgt cgccgcgttc gacgccgacc gcgacgcggt
     6601 ggtcgcgcgc gccgcggccg ccggcgtgct cggcgtcg
//
LOCUS       DASVOR010000926         1503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67564,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000926 DASVOR010000000
VERSION     DASVOR010000926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1503
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67564"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VF516_17960"
     CDS             complement(<1..297)
                     /locus_tag="VF516_17960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109624.1"
                     /translation="MFVCPECGLTQPVPGPCPTDRTPLSPISEDVLLGTTIGAYRVAR
                     LLGIGGMGRVYKGVHPTIGSRVAIKVLSRECSDRRDLVERFFAEAKAVNLIRHES"
     gene            complement(352..804)
                     /locus_tag="VF516_17965"
     CDS             complement(352..804)
                     /locus_tag="VF516_17965"
                     /inference="COORDINATES: protein motif:HMM:NF008742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEX8109625.1"
                     /translation="MNAIANLIWFVLGGLVMGLGWCLAGVVMLVSIIGVPWARACFVF
                     AGFCFFPFGREAIDRRELTGKEDLGTGVLGLIGNMIWLAVAGLWLAIGHITAALLNFA
                     TIIGIPFGIQHLKLAACALWPIGKTVVPKHVAHEARRRHAMMHARRAG"
     gene            complement(883..>1503)
                     /locus_tag="VF516_17970"
     CDS             complement(883..>1503)
                     /locus_tag="VF516_17970"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8109626.1"
                     /translation="PDIAAVFAADWAFAARAQLAPPAEPALAGGAAIQVVGSGPDVRD
                     DLLYDAFLSAVFEARERIWIATPYFVPDSALIRGLVLAARRGVDVRVVVPGRSNHITA
                     DYAGASYLRELAAAGGRICCYEPGMLHAKCVLVDHALAVIGSANLDMRSLFLNYEIAL
                     FLSSPAEIAVSAAWFDTLWPSCGALRPATRPRLLVESVARLIGPLE"
BASE COUNT          258 a          550 c          516 g          179 t
ORIGIN      
        1 gctctcgtgg cggatcaggt tgaccgcctt ggcctcggcg aagaaccgct cgaccaggtc
       61 gcggcggtcc gagcactcgc gcgacagcac cttgatcgcg acccggctgc cgatcgtcgg
      121 gtgcacgccc ttgtagaccc ggcccatccc gccgatcccg agcagccgcg cgacccggta
      181 ggcgccgatc gtcgtgccga gcagcacgtc ctcgctgatc ggtgacagcg gtgtgcggtc
      241 ggtcgggcac gggcccggga ctggctgcgt caggccacac tcgggacaga cgaacatcgc
      301 gatagaatat catgcaggcg gacggatgca gcgggccacc aggtgtcgac gtcagcccgc
      361 gcgacgggcg tgcatcatcg cgtggcgccg ccgcgcctcg tgcgcgacgt gcttgggcac
      421 cacggtcttg ccgatcggcc agagcgcgca cgccgccagc ttcaggtgct ggatcccgaa
      481 cgggatgccg atgatcgtgg cgaagttgag cagcgccgcg gtgatgtggc cgatcgcgag
      541 ccagagcccg gcgaccgcga gccagatcat gttgccgatc aggccgagca ccccggtgcc
      601 gagatcctcc ttcccggtca gctcccggcg atcgatcgcc tcgcggccga acgggaagaa
      661 gcagaacccg gcgaacacga agcacgcgcg cgcccacggg accccgatga tcgagaccag
      721 catgaccacc ccggcgaggc accagccgag gcccatcacc aggccaccca ggacaaacca
      781 gatcagattg gcaatcgcat tcatgtccgc cccctgaaag acagcgtagc gcggcacgcg
      841 aggctcgccc agcaggtcgt caccatgagc gccggacggc gctcactcca gcgggccgat
      901 caaccgcgcg accgactcga cgagcagacg cggcctcgtc gccggccgga gcgcgccaca
      961 gctcggccac agcgtgtcga accacgccgc gctgacggcg atctccgcag gggacgacag
     1021 gaacagtgcg atctcgtagt tcaggaacag gctgcgcatg tcgaggttgg cgctgccgat
     1081 caccgcgagc gcgtggtcga ccagcacgca cttggcgtgc agcatcccgg gctcgtagca
     1141 acagatccgg ccgcccgcgg cggcgagctc gcgcaggtag ctcgcgcccg cgtagtccgc
     1201 ggtgatgtgg ttcgagcgcc cgggcacgac gacgcggacg tccacgccgc gccgcgccgc
     1261 cagcaccagc ccgcggatca gcgcgctgtc gggcacgaag tacggcgtgg cgatccagat
     1321 ccgctcgcgc gcctcgaaca ccgccgacag gaacgcatcg tacagcaggt cgtcgcgaac
     1381 gtccgggccg ctgcccacga cctggatcgc cgcgccgccg gccagggccg gctccgccgg
     1441 cggagcgagc tgcgcgcgcg ccgcgaacgc ccagtccgcg gcgaacaccg cggcgatgtc
     1501 ggg
//
LOCUS       DASVOR010000927         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_155471,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000927 DASVOR010000000
VERSION     DASVOR010000927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155471"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..3683
                     /locus_tag="VF516_17975"
     CDS             339..3683
                     /locus_tag="VF516_17975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109627.1"
                     /translation="MRVTLGAVLVCLAACAPDFDATRTSDSNSLGERMVALLCKRLAF
                     QAEPTDVRGDHFRDACAGGAIPADAPPTLVALLTKRPALVKAIDTAAPDAFTDELQAF
                     LTSDATLALYDNDTMSRSIASLANLLDEVSHDDAAVAALARVATRDGYRPAAVAFGIP
                     AALALARSSASGAPGTPSLHAVLTSTVPAITAGGAAHAEWDALVAALSATLVDDSPPS
                     DAGSPERTLALARDFLLTEHPDLSEPAPIPLVRRDPRGIAQVALAGGAIPAPFVDADG
                     DQLADVDALGRFVDARGAPLTPAPPAPFAIAGDTAPRDAQGRASMYDYVDLSKTVIGA
                     VGHEVSRLLDPAGGPSGSIAFDVMRGASALLGPRVTATRAFTAAPFTYLGYDTMHSPL
                     LDMLYGWIQLLRDPSIGDTLELGETLLADHSAAAARFLEAAITTARLGDAHPEAQILP
                     GAPMWDELMPIVRQILAQPALVQALFNAFQQPQVKDLGLRFHDLLTYSDRFDIDPSKP
                     NQPPPAIFTQMPDRTRPDSGFNRTLFQRFLHVIHDSNRVQMCNKAGATVSFAGTNFGP
                     FAACALIQIDNLATFYLDAIAFAKDASGNPVCEASNGSVVACPPPGQPFPVGVRLRPG
                     ATLVFKDPTLARTIDTSGDQALELTSTITGFRRHPTPPALNRVLFLNPTPAFLATAID
                     PAVDASGALFRDHHPGTLPVLEVKNFYSDIRPIIQAFVDNGDEQLFVDLMSVMHKYWP
                     SKDSTDTQTTDPAAPNYAFGANARSWEPLLADGAAGDLLPALVDNAPELNAITVNGKP
                     FATVLTSAASFAINPLPGLTDRQGRTTTTTADGQPVTQLTPWHVLADAYLARRARLDA
                     TGAEGKAWESAVSGVVDVLFRASNDGSGWAFRNPRVRPVSHWVIEFLRGRIAAHNQAG
                     DLTTWLTRTLPDNATDTLTHPVLAAAADLGVKLTAQGAPRAAPRTAIEALLRDVFDEA
                     ASPALFGMLRTASGDLIQLALDDGDRVPVAHLAGRLIAPDKPYLATQLNLLQNLAAAD
                     RDAVLARLAGQLFTGYDTADPGVPALAAIANGTGEVDRRRPGIDLGADWTKDDFVSVL
                     GNIAAFLRDQQRGFPRFISIIKGRNP"
BASE COUNT          536 a         1397 c         1280 g          550 t
ORIGIN      
        1 gcgcgctcgg ccggcggcgg ttcgtcgagc ccggcccggt cgagtacggc ccgtcgcgga
       61 tcgagtcgcc cgaggacgga catggcgatg gtacgacgca accggtgcgc ggacccgggc
      121 ccaggcagcc cgccgggaag ccgtcgcccg gcagcccgcg gtgacccgcg gcggcggcat
      181 ccgtcacgaa ggccgctgcg tggggcgcgg ggatcgcggg tccggcccga ccgaagtgag
      241 cgggtttatt gaccattctt caatagggac gtggttaaca tatttctctg gagatttgag
      301 tagatggaag ggggcgaccc ctggcacagt ccgctgccat gcgggtcact ctcggagccg
      361 tcctggtctg cctcgcggcg tgcgcacccg atttcgacgc gacgcgcacg tccgattcga
      421 actcgctcgg cgagcggatg gtcgcgctgc tgtgcaagcg tcttgcgttt caggccgagc
      481 ccaccgacgt gcgcggcgat cactttcgcg acgcgtgcgc cggtggcgcc atcccggccg
      541 acgcgccgcc cacgctggtc gcgctgctga ccaagcgccc ggcgctggtc aaggcgatcg
      601 acaccgcggc tccggatgcg ttcaccgatg agctgcaggc gttcctgacc agcgatgcca
      661 ccctggcgct gtacgacaac gacacgatgt cgcggtcgat cgcgtcgctg gccaatctgc
      721 tggacgaggt ctcgcacgac gatgccgcgg tggccgcgct ggcccgcgtg gccacgcggg
      781 acggctatcg ccccgccgcc gtggcgttcg gcatcccggc ggcgctggcc ctcgctcgat
      841 cgagcgcctc gggcgcgccc ggcacgccga gcctccatgc cgtgctcacc agcaccgtcc
      901 ccgcgatcac cgccggaggc gcggcgcatg ccgagtggga cgcgctggtc gccgcgctgt
      961 cggcgaccct ggtcgatgac tcgcctccga gcgacgccgg gtcacccgag cgcaccctgg
     1021 cgctggcccg ggacttcctc ctgaccgagc acccggatct gtccgagccg gcgcccatcc
     1081 cgctggtccg ccgcgatccg cggggcatcg cgcaggtcgc gctcgccggt ggcgcgatcc
     1141 cggcgccgtt cgtcgatgcc gacggcgacc agctcgccga tgtcgacgcg ctcggccggt
     1201 tcgtcgatgc ccggggcgcg ccgctcaccc cggccccgcc ggcgccgttc gcgatcgccg
     1261 gtgacacggc accccgcgac gcgcagggca gggcgagcat gtacgactat gtcgacctgt
     1321 cgaagaccgt gatcggcgcg gtcggacacg aggtgtcccg gctgctggac ccggccggag
     1381 ggccgtccgg cagcatcgcg ttcgacgtca tgcgcggcgc atctgcgctg ctcggcccgc
     1441 gcgtcaccgc gacccgcgcg ttcaccgccg caccgttcac ctacctcggc tacgacacca
     1501 tgcactcgcc gctgctcgac atgctctacg gctggatcca gctgctgcgc gatcccagca
     1561 tcggtgacac gctggagctg ggagagacgc tgctcgccga tcacagcgcc gcggcggcgc
     1621 gcttcctcga ggccgcgatc accaccgccc gtctcggcga cgcgcacccg gaggcccaga
     1681 tcctgccggg cgcgccgatg tgggacgagc tgatgccgat cgtgcgccag atcctcgccc
     1741 agccggcgct ggtccaggcg ctgttcaacg cgttccagca gccccaggtc aaggacctgg
     1801 gactgcggtt ccacgatctc ctgacctaca gcgaccgctt cgacatcgat ccgagcaagc
     1861 cgaatcagcc gccgccggcc atcttcaccc agatgcccga ccggacccgg ccggacagcg
     1921 ggttcaaccg gacgctgttc cagcggttcc tgcacgtgat ccatgactcg aaccgcgtgc
     1981 agatgtgcaa caaggcgggc gcgacggtgt cgttcgcggg gaccaacttc ggcccgttcg
     2041 cggcctgcgc cctgatccag atcgacaacc tcgcgacgtt ctacctcgac gccatcgcgt
     2101 tcgcgaagga tgccagcggc aatcccgtgt gcgaagcgtc gaatgggagc gtcgtggcat
     2161 gccccccccc gggtcagccg ttccccgtgg gggtgcggct tcgaccgggt gcgaccctcg
     2221 tgttcaagga tcccacgctc gccaggacca tcgacaccag cggtgaccag gccctggagc
     2281 tgacctcgac gatcacgggc ttccgccgcc accccacgcc tccggcgctc aaccgggtgc
     2341 tgttcctgaa cccgacgccg gccttcctgg ccacggcgat cgatccggcg gtcgatgcga
     2401 gcggcgcgct gttccgggat caccaccccg gcacgctgcc ggtgctcgaa gtaaagaact
     2461 tctacagtga tatccggccg atcatccagg cattcgtcga caacggcgac gagcagctgt
     2521 tcgtcgatct gatgtcggtg atgcacaagt actggccgag caaggactcg accgacacgc
     2581 agaccaccga tccggccgcc ccgaactacg cgttcggcgc caacgcccgg tcgtgggagc
     2641 cgctgctcgc cgatggcgcg gccggcgacc tgctgccggc gctggtcgac aacgcccccg
     2701 agctcaacgc gatcacggtc aacggcaagc cgttcgcgac ggtgctcacc agcgccgcga
     2761 gcttcgcgat caacccgctt cccgggctca ccgatcgcca gggccggacc acgacgacga
     2821 ccgccgatgg ccagccagtc acccagctga caccgtggca cgtgctcgcc gacgcctacc
     2881 tcgcccggcg ggcgcgcctg gacgccaccg gggcggaggg caaggcgtgg gagtctgccg
     2941 tgtcgggcgt ggtcgatgtg ctgttccggg ccagcaacga cggctccggc tgggcgttcc
     3001 gcaacccccg cgtccggccc gtgagccact gggtgatcga gttcctgcgc gggcggatcg
     3061 ccgcccacaa tcaggccggc gatctcacga cgtggctgac gcggaccctc ccggacaacg
     3121 cgaccgatac cctgacccat ccggtgctgg cggccgcggc cgatctcggg gtcaagctca
     3181 ccgcgcaggg cgcgcccagg gccgcgccca ggactgcgat cgaggcgctg ctgcgcgacg
     3241 tgttcgatga ggccgcgtcc ccggcgctgt tcggcatgct gcgcaccgca tcgggagacc
     3301 tgatccagct cgcgctcgac gatggcgatc gcgtgccggt cgcgcacctc gcggggcggc
     3361 tgatcgcacc ggacaagccg tacctggcca cccagctcaa cctgctgcag aacctggccg
     3421 ccgccgaccg ggatgcggtg ctggcgcggc tcgccggcca gctgttcacc ggctacgaca
     3481 cggccgatcc aggcgtgccg gcgctcgcgg cgatcgccaa cgggaccggc gaggtcgatc
     3541 gccggcgccc cgggatcgat ctgggcgccg actggacgaa ggacgacttc gtgagcgtgc
     3601 tcgggaacat cgccgcgttt ctgcgcgacc agcagcgcgg ctttccccgc ttcatctcga
     3661 tcatcaaggg acggaaccca tgaaccgtct cgctgcgctg gttgcgatct ctctgggcgt
     3721 gggcgcgtgg gcgggcgagg cgcgcgcggg cgggatgctg ctg
//
LOCUS       DASVOR010000928         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447411,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000928 DASVOR010000000
VERSION     DASVOR010000928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447411"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..722
                     /locus_tag="VF516_17980"
     CDS             516..722
                     /locus_tag="VF516_17980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109628.1"
                     /translation="MRTTTLGGGAAASAGFDQLQLTQSRRSIQNRSNRGSPLDTADMM
                     RRMVLLAGNPGGSSVHASYGGKGR"
     gene            730..1890
                     /locus_tag="VF516_17985"
     CDS             730..1890
                     /locus_tag="VF516_17985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8109629.1"
                     /translation="MEARTSDLVTATARIAAEVAAKHAADVDSGARFPRETFAALKEA
                     KLLSAAVPRSHGGHGAGMLELGRQCAALAHGCSSAAMILAMHHIQVACLARHGAGTPF
                     FDAYLRDHVVQRQELIASITSENGTFGDTRSSICAVEVAGDRIALDKDATTVSYGEHA
                     DAQLVTCRRAADAPASDQVLVLFRRADYTLAPTGTWDTLGMRGTCSPGAKFAGRGGVE
                     QIVPGSFADSSAQTMVPYSHILWSALWYGIAADAVARAAQFVRAEARKKPGVVPPQAT
                     RLARVHVDLQAMRHNWEGCARELDDLGDDRGPLDGMGWALKLNQLKMSCSELAPRIVH
                     EALQIIGILAYKNDSKFSVGRHYRDVLSASLMVSNERIAGKSASMLLVFKDE"
     gene            1890..2798
                     /locus_tag="VF516_17990"
     CDS             1890..2798
                     /locus_tag="VF516_17990"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: protein motif:HMM:NF005479.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HEX8109630.1"
                     /translation="MPATDYDATRFFDGLVRAGLIVPVGVPGVFGRNHVFEDVLARFD
                     AQLARLADGADYFVYPPVIDRRVLEKADYMDSFPHLAGTVHSFSGKELDARMLSARIH
                     AGEPWGDAMTITNVTLNPAACYPVYPSFTGVVPADGRLVTMVNWVFRHEPSHEPTRMQ
                     AFRVRELVRCGTPAQVVAWRDAWLDRGVAFLASLGLPVTSDVAADPFFGKGGRMLKQN
                     QRDQKLKFELLVPVISTTEPTAVCSFNLHETHFGDAFGIRTPDGAIAHTACLGFGLER
                     CVMALFQTHGFDPAAWPSAVRGKLWP"
     gene            2795..>3154
                     /locus_tag="VF516_17995"
     CDS             2795..>3154
                     /locus_tag="VF516_17995"
                     /inference="COORDINATES: protein motif:HMM:NF020467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1839 family protein"
                     /protein_id="HEX8109631.1"
                     /translation="MKALDLDATRHARHALHADDRAWVEKNCYVDIWIEVIHALGCEP
                     LAIAPFVVALDFEGDQWTFFKPPHDELRELYGIDVQELNCWRPLVEHAVEHVGAGKLI
                     STEADAWWLPDVAGTDYR"
BASE COUNT          454 a         1116 c         1125 g          459 t
ORIGIN      
        1 ggagacctgg aagtcagacg cgctgccgcg gcaaggtgcg atcgtcaagt cgctgcaccc
       61 cgacctaccg gggaccgtcg aggagatcga agccgactac gccgcgcgct gcgccaagac
      121 gctgtacggc tgagcgcgga ctcagtcgcg agcgagccag atctcgacct cgcgctcgac
      181 ccagcgccac ttgcagatca gcggctggcg tgcggccgag ggacccgcgc ggccaggtcg
      241 actggccccg ccggcgcccc gggcccccag cgcggccctg tgttgccgca ccgcctcgaa
      301 cccccccggt cacttcgcgt gtgaaccgcg ttcccggggt gcgacggccc catgtagctg
      361 ttttttcgtg gccggccgct ggcgcggtcg gcctcggccg gcctgtatcc ccgaggttac
      421 gtctcggtga tacgataccc acgcgagaca ccccaagccc gcgtcggatc gggctcctgg
      481 ccctggcacg cgcggtgcct tgggaattag tcaccatgcg taccaccacc ctcgggggag
      541 gggcggccgc gtcggccggc ttcgatcagc tccagctcac gcagagccgg cgctcgatcc
      601 agaaccggtc gaatcgcggc agcccgctcg acacggcgga tatgatgcgg aggatggtgc
      661 tgctcgccgg caacccgggc ggctcgtcgg tccacgcgtc gtacggcggc aagggccggt
      721 gatcgcgtca tggaggcccg aaccagcgat ctcgtcaccg cgaccgcgcg gatcgccgcc
      781 gaggtcgcgg cgaagcatgc cgcggacgtc gacagcggtg cacggtttcc gcgcgagacg
      841 ttcgccgcgc tgaaggaggc gaagctgctg tcggcggcgg tgccgcggtc gcacggcgga
      901 cacggcgcgg gcatgctcga gctcggtcgc cagtgcgccg cgctcgcgca cggctgcagc
      961 agcgccgcga tgatcctcgc gatgcaccac atccaggtcg cgtgcctcgc gcgccacggc
     1021 gccggcacgc cgttcttcga cgcgtacctg cgcgaccacg tcgtgcagcg ccaggagctg
     1081 atcgcgtcga tcacgtcgga gaacggcacg ttcggcgaca cgcggtcgtc gatctgcgcg
     1141 gtcgaggtcg ccggcgatcg catcgcgctc gacaaggacg cgacgacggt gtcgtacggc
     1201 gagcacgccg atgcgcagct cgtgacctgc cgccgcgcgg cggacgcccc cgcgagcgat
     1261 caggtgctcg tgctgttccg gcgcgccgac tacaccctcg cgccgaccgg cacgtgggac
     1321 acgctcggca tgcgcggcac gtgcagcccc ggcgcgaagt tcgcggggcg gggcggcgtc
     1381 gagcagatcg tgcccggctc gttcgcggac tcgtcggcgc agaccatggt cccgtactcg
     1441 cacatcctgt ggtcggcgct gtggtacggc atcgccgcgg atgcggtcgc gcgcgccgcg
     1501 cagttcgtcc gcgccgaggc gcgcaagaag ccgggcgtcg tgccgccgca ggcgacgcgg
     1561 ctcgcgcgcg tgcacgtcga cctgcaggcg atgcggcaca actgggaggg ctgcgcgcgc
     1621 gagctcgacg atctcggcga cgaccgcggc ccgctcgacg gcatgggctg ggcgctcaag
     1681 ctcaaccagc tcaagatgag ctgctcggag ctcgcgccgc ggatcgtcca cgaggcgctg
     1741 cagatcatcg gcatcctcgc ctacaagaac gactcgaagt tcagcgtcgg acgccactac
     1801 cgcgacgtgc tgtccgccag cctgatggtc tccaacgagc ggatcgcggg gaagagcgcg
     1861 tcgatgctgc tcgtattcaa ggacgaatga tgccggccac cgactacgac gccacgcggt
     1921 tcttcgacgg gctggtccgc gccggcctga tcgtcccggt cggcgtgccc ggcgtgttcg
     1981 gccgcaacca cgtgttcgag gacgtgctcg cccggttcga cgcgcagctc gcgcggctcg
     2041 ccgacggcgc ggactacttc gtgtacccgc cggtgatcga tcgcagggtg ctcgagaagg
     2101 ccgactacat ggacagcttc ccgcacctcg cggggaccgt gcacagcttc tccggcaagg
     2161 agctcgacgc ccgcatgctg tcggcgcgga tccacgccgg cgagccgtgg ggcgacgcga
     2221 tgacgatcac caacgtcacg ctgaacccgg ccgcgtgcta cccggtgtac ccgagcttca
     2281 ccggcgtggt gcccgcggac ggccgcctgg tgacgatggt gaactgggta tttcgccacg
     2341 agccgtcgca cgagccgacg cggatgcagg cgtttcgcgt ccgcgagctc gtgcgctgcg
     2401 ggacgccggc gcaggtcgtc gcgtggcgcg acgcgtggct cgatcgcggc gtggcgttcc
     2461 tcgcgtcgct cgggctgccg gtgacgtccg acgtcgcggc ggatccgttc ttcggcaagg
     2521 gcgggcgaat gctcaagcag aaccagcgcg atcagaagct gaagttcgag ctgctggtcc
     2581 cggtgatctc gacgaccgag ccgaccgcgg tgtgctcgtt caacctgcac gagacgcact
     2641 tcggcgacgc gttcggcatc cgcacgcccg acggcgcgat cgcgcacacc gcgtgtctcg
     2701 gcttcgggct cgagcgctgc gtgatggcgc tgttccagac ccacggcttc gatccggcgg
     2761 cgtggccgag cgcggtccgg gggaagctgt ggccgtgaag gcgctcgacc tcgatgccac
     2821 gcgccacgcg cggcacgcgc tgcacgccga cgaccgcgcg tgggtcgaga agaactgcta
     2881 cgtcgacatc tggatcgagg tgatccacgc gctcggctgc gagccgctgg cgatcgcgcc
     2941 gttcgtcgtc gcgctcgact tcgagggcga tcagtggacg ttcttcaagc cgccgcacga
     3001 cgagctccgc gagctgtacg ggatcgacgt ccaggagctg aactgctggc ggccgctcgt
     3061 cgagcacgcc gtcgagcacg tcggtgccgg caagctgatc tcgaccgagg cggacgcgtg
     3121 gtggctcccc gacgtcgcgg gcaccgacta ccgg
//
LOCUS       DASVOR010000929         2216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417834,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000929 DASVOR010000000
VERSION     DASVOR010000929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417834"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(297..1343)
                     /locus_tag="VF516_18000"
     CDS             complement(297..1343)
                     /locus_tag="VF516_18000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109632.1"
                     /translation="MAGWIHSRQLEVIDFLREENRVLREQLGGRRLRFTDDQRRRLAV
                     KGRVVGRRRLGEFAGLVTPDTILRWYRELIARKYDGSARHRIGRPTVAADLEQLVVRM
                     ATENPTWGYTRLVGALGNLGHQIGRNTVKRVLLRHGLEPAPARGKRMPWKTFLRTHLG
                     VIAAADFFSVEVLTLMGLVRYFVLFVIDLQTRHVQIGGVVRQPYGAWMTQVARNLTDG
                     VDGFLIGKRYLLHDRDPLFTDEFRGVLGAAGVKCLRLPARSPDLNSVAERFVLSIKSE
                     CLSKLVPLGERHLRRAVSEYMVHYHLERNHQGVGNRLLTPAEQAVPSANSNAPIERRE
                     RLGGLLNFYYRRAA"
     gene            complement(1484..>2216)
                     /locus_tag="VF516_18005"
     CDS             complement(1484..>2216)
                     /locus_tag="VF516_18005"
                     /inference="COORDINATES: protein motif:HMM:NF019109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1517 domain-containing protein"
                     /protein_id="HEX8109633.1"
                     /translation="LAAGLAVLAVFVMAPRRPRTWRGAPREAHVAVLHVAFAPAARTA
                     LTAALPEIARDAKPARAEGRAALVHELSVLLRRHAKDAAYVGCELVAATDRSSARSEF
                     ERHALEARARFVEERVRNDGEHVVEQVAPGVERERSGFTLVTLVLATRKPLLEPVRDR
                     ASLDAALRQLGNVLPFAVFACEVIWMPADRGEALSSLFIEDQLPHLTKLPGAIAGPCT
                     CAYCGARYPIEDAMCPRCGAHPVAT"
BASE COUNT          352 a          785 c          762 g          317 t
ORIGIN      
        1 cgtcgtgctc aagccactac gccggcagat gctacggcta accctgaagt aagcgcccgt
       61 gagcccgtgg cgtagatcag cgtcggccag cggcgccaag ctgggcgcgc cgctggcggg
      121 cagccagtcg atgaccggcg cgaaggccgg ctcggcggag atgtccgcga agctgttgat
      181 gatgagcccg ccgcgccacg ttcggtgcgc cagcgccctc gtccggccga tccggcgcag
      241 cagctcgacc ggcggcggga tcggcccccg tcatgtgcca gaagtggatc gcggactcat
      301 gcagcgcggc ggtagtagaa gttgagcagc cctccaagcc gttcgcggcg ctcaatcggc
      361 gcgttgctgt tggccgatgg caccgcctgt tcggctggcg tgagcagccg gttgcccacg
      421 ccctggtggt tgcgctcgag gtgatagtgc accatgtact ccgacaccgc acgccgcagg
      481 tggcgttcac ccagtggcac cagcttgctc aagcactctg acttgatcga gagcacgaag
      541 cgttcggcga cggaattcag gtcgggactg cgcgcaggga ggcgtaggca ctttacccct
      601 gccgctccga gcacgccgcg gaactcgtcg gtgaacagcg ggtcccggtc gtgaagcagg
      661 taccgcttgc cgatcagaaa cccgtccaca ccgtcggtga ggttgcgtgc gacctgcgtc
      721 atccacgcgc cgtacggttg ccgcaccacg ccgccgatct ggacgtgccg ggtctggaga
      781 tcgatcacga acagcacgaa gtagcgaacc agccccatca gcgtgagcac ctcaaccgag
      841 aagaagtccg cggcggcgat cacgcccagg tgcgtgcgaa ggaatgtctt ccacggcatc
      901 cgcttgccgc gcgcgggtgc gggctcgagg ccgtgccgga ggaggacgcg cttgacggtg
      961 ttgcggccga tctgatggcc gaggttaccg agcgcgccga ccagccgcgt gtagccccag
     1021 gtcggattct ccgtcgccat ccggacgacg agctgctcca ggtcggcggc gacagtaggg
     1081 cgcccgatcc ggtgacgcgc cgagccgtcg tacttgcggg cgatcagctc gcggtaccag
     1141 cgcaggatcg tgtcgggcgt gaccagcccc gcgaactcgc cgagcctgcg acggccgacg
     1201 accctgccct tgacggccag gcgtcgccgc tggtcgtcgg tgaagcgtag gcgccgtccg
     1261 ccgagttgct cgcggagcac gcggttctcc tcgcgaagga agtcgatgac ctcgagctga
     1321 cgggagtgga tccagccggc catccagagg accaagaacc gcacagcttc ggtgcccatc
     1381 gcggcaccat ggtagcgcgg ccggtgatcg acctggggcc cgcgacgagc acggcaactt
     1441 ggatacgcat cgcatttcct tccggcgggc tttcggcaca cgactacgtc gcaaccggat
     1501 gcgcgccgca ccgcggacac atggcgtcct cgatcggata ccgcgcgccg cagtaggcgc
     1561 acgtgcacgg tcctgcgatc gcgcccggta gcttcgtcag gtgcggcagc tgatcttcga
     1621 tgaagagcga cgacagcgcc tcgccgcgat ctgccggcat ccagatcacc tcgcacgcga
     1681 acaccgcgaa cggcaggacg ttcccgagct gccgcaatgc cgcgtcgagg ctcgcgcggt
     1741 cgcgcacggg ctcgagcagc ggcttgcgcg tcgcgagcac cagcgtcacg agcgtgaagc
     1801 cgctgcgctc gcgctcgacg cccggtgcga cctgctcgac cacgtgctcg ccgtcgttgc
     1861 ggacccgctc ctcgacgaat cgtgcccgcg cttcgagcgc gtggcgctcg aactcgctcc
     1921 gcgcgctcga gcgatcagtc gcggccacga gctcgcagcc gacgtacgcg gcgtccttcg
     1981 cgtgacgccg cagcagcacc gagagctcgt gcacgagcgc agcgcggccc tcggcgcgcg
     2041 ccggcttcgc atcacgcgct atctccggca gcgccgccgt cagcgcagtt cgcgccgcgg
     2101 gtgcgaacgc gacgtgcagg accgcgacat gcgcctcgcg cggcgcaccg cgccacgtcc
     2161 gcggccggcg cggcgccatc acgaacaccg cgagcacggc gagcccggcc gcgagc
//
LOCUS       DASVOR010000930         7317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_126098,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000930 DASVOR010000000
VERSION     DASVOR010000930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126098"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..390)
                     /locus_tag="VF516_18010"
     CDS             complement(<1..390)
                     /locus_tag="VF516_18010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109634.1"
                     /translation="MTARRTLGVALGLALHLALGLGLALLGAACHSSETLTPPPPPLR
                     PEPEASKPKATAATQKDCDPVDAGREPKPLSFDERSLPEGYRLAEQGRSKLRTAQSAE
                     VTRATREDMITQAVDAFITALRADPYNI"
     gene            complement(387..983)
                     /locus_tag="VF516_18015"
     CDS             complement(387..983)
                     /locus_tag="VF516_18015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109635.1"
                     /translation="MVRGSIGARLGVGLAPWVFVFGVLGGVLGSAGCQPLYGGKPEKL
                     PTPARKKPPKDEPVAEAPVKMIDDCTADFRGNPATVRQQTNQSNDLTDQGSTALANSD
                     KTKDGGAQASLLRDAIDKLRNALVKDPYNAAATLKLALAYDKVYRKGCAIAMLKRLAT
                     LAANPKFSRVANAEIDSISDNGQWFKHYRKDAMTAVGR"
     gene            complement(1066..1785)
                     /locus_tag="VF516_18020"
     CDS             complement(1066..1785)
                     /locus_tag="VF516_18020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109636.1"
                     /translation="MKLVGERRALAAAIMAFYFLLYGVMAVSQLAPDFTKAFAAISGV
                     YGLAFFALVAGYFWARWFGVGVGLYGVITAAVGIWQLGAEPILLFIGGTHLAATLFLW
                     GEAMSQPYDGQAAWREKFHMDDSAVQRLGRSVIRAGVSLPFILLYAFMPRPEPASMAV
                     SVVAMVLAVAGLRGLLRLKTWGVLALGAAGALTLAVAGADVAAHGLSPWMLRPVLAGS
                     LMFSAVAPYLRPILRFVFAAA"
     gene            complement(1817..2407)
                     /locus_tag="VF516_18025"
     CDS             complement(1817..2407)
                     /locus_tag="VF516_18025"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8109637.1"
                     /translation="MAFTQREARRLIRKAVGRVPEGEEIRHLNIMPMMDMMTILLVAF
                     IYQAAVSATQLLAGTVSLPRSLTADDLPENATTVVITKTGIVVASDKAKTIVPVSNGD
                     VDAGEKEEGATGIKIPRLTAYLAALHHEEEAVRQRQPGFDPAHAPPPELLLIADRSTP
                     YHLLIAVLYSAKQKEAGYQHFRLIVQKSFPNAPPRK"
     gene            complement(2422..3234)
                     /locus_tag="VF516_18030"
     CDS             complement(2422..3234)
                     /locus_tag="VF516_18030"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8109638.1"
                     /translation="MEYSAQRVRAKTRAAIKRREDAIEQDELESGELNLIPYLDMVTN
                     LMLFLLASVSAGLILVQINTTLPDKKADTAPATPTPATSPDDQPLKLFVSIARDRVVL
                     WSLTGLEGTLKQPKLVLLRTGREGEACDGAYMCESNTCDNTTQKCVAGHDDSAPVFDY
                     RALNNALFEIASRRYAGRPRKTDTYPAVLQADGDIPYSTIVSIMAAMRCKLPDFGKEA
                     MPCALPTEDPALKKAPDPISPDGKLYDTARASYDPSKMALFPDIQFSTGFEG"
     gene            complement(3245..3919)
                     /locus_tag="VF516_18035"
     CDS             complement(3245..3919)
                     /locus_tag="VF516_18035"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8109639.1"
                     /translation="MNWLSDVFQKGGPFFYLNTFFMAIVVGLVVERAIYFLGRGHLNA
                     KAFLEQLRKLLSANNVDRAKKLCDATTAPVARVAKAGLNRLHRGEAAVAQAMEETMTD
                     TLPEVKTRIGALWSLANIVTLVGLLGTITGLIHTFASIGNANPADRQKQLADGISEAM
                     YNTAFGLTIALICMVAHLVLSGAMKKVVADLEAFSLRFENLLADGGAAAQSRAPAAPT
                     DDEHGA"
     gene            complement(4075..4590)
                     /locus_tag="VF516_18040"
     CDS             complement(4075..4590)
                     /locus_tag="VF516_18040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109640.1"
                     /translation="MLRLVAIASLAVGLPWGCRISLEDNVALTADGGSNGRACTVSTT
                     SQPCMDATTHSDLAFIEQKIFAPSCNFSGCHDSATDSGKLDLRAGVSHDHLVGVASQI
                     DTTRKLVVPNDAAASYLMLMLRDVAPAMASPPGNPPPGSVGFMPQGAPTLCCQKLDAI
                     ERWINAGAPNH"
     gene            complement(4766..>7317)
                     /locus_tag="VF516_18045"
     CDS             complement(4766..>7317)
                     /locus_tag="VF516_18045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEX8109641.1"
                     /translation="AAGAMATPAATATAATSGVACSHRRDLIDSEHTVAEPAIPRKRR
                     RRRRLRTVSVLRMGFLLAIYGASFAAAAIYLAVQTINNELPKDLSQLLDYQPSRKSVV
                     LSSDGEEIGAFSIENRRIVPLDRMPPHVPAAFLSAEDRRFYKHKGFDLLGIARAAITN
                     FRSDGNIKQGGSTITQQIIKQTLLAGEEQIGALDLTPAEYAKERKARKYRRKAKEVIL
                     AIRLERELTKAEILSIYLNHVYLGHGAYGVGAAAQTYFGKEVEDLTIAEAAMLGGLVA
                     SPTKYAPHRNMQLARERQRYVLGHMRDDHYISNAEYEAALAEPIALVDESDLNHLASP
                     YFVEHIRQLATRRYGNRDLFRGGLKFYSTIDTRMQSAAESALRRGLEALDRKLGFRGP
                     IGAIAPAARAAWTGGPAHPISGPTDDVAAVADQVLPEQVYGAMVVDLPRSGAGVTLDL
                     GPRRMALIDADARDVRAFRDARTGKPVALGDLLPVRLSPDGNQAVLAQRPALQGSFIA
                     IEPGTGRVLALVGGYDWTASQFDRATQAKRQVGSSIKPFIYSAALEAGKTPVDRLVDG
                     PFSVTTATGVWTPANYDNKYLGDVTLMTALAYSLNTISVQLAVSVGLDRIIEILRGYG
                     ITSPIPRHISISLGTPDLTPLEVATGYAGIASGGRRITPRFFDLVTDTAGNVVEDLRQ
                     QPPGPQVVSPDVDYVVTNLMKGVVARGTARFAAQLGRPIAGKTGTSANYRDVWFNGFT
                     TDLVGSVWIGRDDSTPIGDKITGGGAAVPIWLDFMQKAHPRTPVRDFSVPPNVSFARV
                     EPWSGDPTGPSTEAVWMPFVRGTLPGKFLAGPAIRAFDDLVPAPVLPVAPR"
BASE COUNT         1044 a         2360 c         2771 g         1142 t
ORIGIN      
        1 gatgttgtac ggatcggcgc gcagcgcggt gatgaaggcg tcgaccgcct gggtgatcat
       61 gtcctcgcgg gtggcgcggg tgacctcggc gctctgcgcg gtgcgcagct tggaccggcc
      121 ctgctcggcg agccggtagc cctcggggag cgagcgctcg tcgaagctga gcggcttggg
      181 ctcgcggccg gcgtcgaccg ggtcgcagtc cttctgggtc gccgcggtgg ccttgggctt
      241 ggacgcctcg ggctcggggc gcagcggggg cggcggcggg gtgagggtct cgctcgagtg
      301 gcaggccgcg ccgagcagcg cgagcccgag gccaagagcg agatggagcg cgaggccgag
      361 agcgacgccg agcgtgcggc gcgccgtcat cggcccaccg ccgtcatcgc gtccttgcga
      421 tagtgcttga accactgccc gttgtcgctg atcgagtcga tctcggcgtt ggcgacccgg
      481 gagaacttgg ggttggcggc cagggtcgcg agccgcttga gcatcgcgat cgcgcagccc
      541 ttgcgataga ccttgtcgta ggcgagcgcg agcttgagcg tcgcggcggc gttgtacggg
      601 tccttgacca gcgcgttgcg gagcttgtcg atggcgtcgc ggagcaggga ggcctgggcg
      661 cccccatcct tggtcttgtc ggagttggcc agcgcggtcg agccctgatc ggtcaggtcg
      721 ttggactggt tggtctgctg ccggacggtc gcgggattgc cccggaagtc ggcggtgcag
      781 tcgtcgatca tcttgaccgg cgcctcggcg accggctcgt ccttcggcgg cttcttcctc
      841 gccggggtcg gcagcttctc cggcttgccg ccgtacagcg gctggcagcc cgcgctaccg
      901 agaaccccgc cgagaacccc gaagacgaag acccacggcg ccagacccac gcccagacgt
      961 gccccgatgg aacctctgac catgtcgagg acggtaaccc ggcgcacccg cagattcaac
     1021 gcggcggcgc agcgagccgc cggggccggc ccggggcggc aatgactacg ccgcggcgaa
     1081 cacgaaccgg aggatcggcc gcaggtacgg ggcgaccgcg gagaacatca ggctgccggc
     1141 cagcaccggc cgtagcatcc acgggctgag gccatgcgcg gccacgtcgg cgccggcgac
     1201 cgcgagggtc agcgcgccgg ccgcgccgag cgccagcacg ccccaggtct tgagccggag
     1261 caggccgcgc aggccggcga ccgcgaggac catggccacg accgagaccg ccatggaggc
     1321 cggctcgggc ctcggcatga aggcgtagag caggatgaac ggcaggctga ccccggcgcg
     1381 gatcaccgag cggccgagcc gctggaccgc gctgtcgtcc atgtggaact tctcgcgcca
     1441 ggcggcctgg ccgtcgtacg gctggctcat cgcctcgccc cacaggaaca gcgtcgcggc
     1501 caggtgggtg ccgccgatga acagcaggat cggctcggcg ccgagctgcc agatgccgac
     1561 cgcggcggtg atgacgccgt acagcccgac gcccaccccg aaccagcggg cccagaagta
     1621 gccggcgacc agcgcgaaga acgccaggcc gtagaccccg gagatcgccg cgaatgcctt
     1681 ggtgaaatcc ggtgcgagct gcgagaccgc catcacgccg tagagcagga agtagaaggc
     1741 catgatggct gccgccaggg cgcgccgttc gccgacgagc ttcatacctt ccattatagc
     1801 gtgaggcgag gagctgtcac ttcctgggtg gcgcattggg gaacgacttc tggacgatga
     1861 gtcggaaatg ttggtagccg gcttccttct gcttggccga gtacagcacg gcgatcagca
     1921 ggtggtacgg cgtgctgcgg tcggcgatga gcagcagctc gggcggcggc gcgtgggccg
     1981 ggtcgaagcc gggctggcgc tggcggacgg cctcctcctc gtggtggagc gcggccaggt
     2041 acgcggtgag ccgcgggatc ttgatgccgg tcgcgccctc ttccttctcg ccggcatcga
     2101 cgtcgccgtt ggacaccggg acgatggtct tggccttgtc gctggccacc acgatcccgg
     2161 tcttggtgat caccacggtg gtcgcgttct cgggcaggtc gtcggcggtc agcgaccgcg
     2221 gcagcgacac ggtgccggcg agcagctggg tcgcgctgac cgccgcctgg tagatgaagg
     2281 cgaccagcag gatcgtcatc atgtccatca tcggcatgat gttgaggtgg cggatctcct
     2341 cgccctcggg gacccggccc accgccttgc ggatcagtcg gcgcgcctcg cgctgcgtga
     2401 acgccatcta ggtcctcatc cctatccctc gaaccccgtc gagaactgga tgtcggggaa
     2461 cagcgccatc ttgctggggt cgtagctggc ccgcgcggtg tcgtacagct tgccgtcggg
     2521 agagatcggg tcgggcgcct tcttgagcgc cgggtcctcg gtcggcagcg cgcacggcat
     2581 ggcctccttg ccgaagtccg gcagcttgca ccgcatcgcg gccatgatcg agacgatcgt
     2641 cgagtacggg atgtcgccat cggcctggag cacggccgga taggtgtcgg tcttgcgcgg
     2701 ccggccggcg tagcgccggc tggcgatctc gaacagcgcg ttgttgagcg cgcggtagtc
     2761 gaacaccggg gcggagtcgt cgtggccggc gacgcacttc tgggtggtgt tgtcgcaggt
     2821 gttcgactcg cacatgtacg cgccgtcgca cgcctcgccc tcgcgcccgg tgcgcagcag
     2881 cacgagcttg ggctgcttca gcgtgccctc gaggccggtc agcgaccaca agacgacgcg
     2941 atcgcgcgcg atcgagacga acagcttgag cggctgatcg tcggggctgg tcgccggcgt
     3001 cggggtggcc ggcgcggtgt cggccttctt gtccggcagc gtggtgttga tctggaccag
     3061 gatgagcccc gccgacaccg aggccagcag gaacagcatc aggttggtga ccatgtcgag
     3121 gtacgggatc aggttgagct caccgctctc gagctcgtcc tgctcgatcg catcctcgcg
     3181 ccgcttgatc gcggcgcggg tcttggcgcg cactctctgg gcggagtact ccatgctagg
     3241 ccatctacgc gccgtgctcg tcgtcggtcg gcgcggcggg ggcgcggctc tgcgcggcgg
     3301 cgccgccgtc ggccaggagg ttctcgaacc gcaggctgaa cgcctcgagg tcggcgacga
     3361 ccttcttcat cgcgccggac agcaccaggt gggcgaccat gcagatcagc gcgatcgtga
     3421 ggccgaacgc cgtgttgtac atcgcctcgg agatgccgtc ggcgagctgc ttctggcggt
     3481 cggccgggtt ggcgttgccg atcgacgcga aggtgtggat caggccggtg atcgtgccga
     3541 gcagaccgac cagggtgacg atgttggcga gcgaccacag cgcgccgatc cgggtcttca
     3601 cctcgggcag cgtgtcggtc atcgtctctt ccatcgcctg ggcgaccgcg gcctcgccgc
     3661 ggtgcagccg gttgagcccg gccttggcga cgcgggcgac cggcgcggtg gtcgcgtcgc
     3721 acagcttctt ggcgcggtcg acgttgttgg ccgacaagag cttgcgcagc tgctcgagga
     3781 acgccttggc gttgaggtgg ccgcggccca ggaagtagat cgcacgctcg acgacgaggc
     3841 cgaccacgat cgccatgaag aacgtgttca agtagaagaa cgggccgccc ttctggaaga
     3901 catcgcttaa ccagttcatc ggtcgatttg cctccgcgcg cgagggcgca cccccgtgcg
     3961 gtacatggga ctgttgtagc gatagactgc ggaacccgtc aagcaaggtc tcggcggaaa
     4021 ctcgttggtt aacaggggtt tactcgtgct gatgggcggg acttgcggca cgggtcagtg
     4081 gtttggagca ccggcgttga tccaacgttc aatcgcatcg agcttttgac agcacaaagt
     4141 gggggccccc tggggcatga agccgacgct gccgggcggt gggttgccgg gagggctggc
     4201 catcgcgggc gcgacgtcgc gcagcatcag catgagatag ctcgcggcgg cgtcgttggg
     4261 tacgacgagc ttgcgcgtgg tgtcgatctg cgacgccacg cccacgaggt gatcatggct
     4321 cacgccggcg cggagatcga gcttgccgga gtcggtggcg ctgtcgtggc atccggagaa
     4381 gttgcacgac ggtgcaaaga tcttctgctc gatgaacgcg agatcggaat gcgtcgtcgc
     4441 gtccatgcag ggctgcgacg tcgtgctgac cgtgcaggcg cggccgttgc tgccgccgtc
     4501 tgccgtcagc gcgacgttgt cctccagcga gatccggcag ccccagggca ggccaacggc
     4561 caggctggcg atcgcgacaa ggcgaagcat ggcgggaacc tagcacgtgc cgcgctgcgt
     4621 cccggcgggc gcgcgatcgg cgcggacatc gatcacaggg cggcacatcg cggcaccccg
     4681 accgaggtcg aggacgccgc gcgggcgcgc aacccgcgta cagcgcgcgg ggtgtcagcg
     4741 cggcggggtg tcagcgcggc gggagtcaac gcggcgcgac cggcagcacc ggcgccggca
     4801 ccaggtcgtc gaacgcgcgg atcgccgggc cggcgaggaa cttgccgggc aaggtgcccc
     4861 gcacgaacgg catccacacc gcctcggtgg acgggccggt gggatcgccg ctccacggct
     4921 cgacccgtgc aaagctcacg ttgggcggca cggagaagtc gcggaccggc gtgcggggat
     4981 gcgccttctg catgaagtcg agccagatcg gcaccgcggc gccgccgccg gtgatcttgt
     5041 cgccgatcgg ggtcgagtcg tcgcggccga tccagaccga gcccacgagg tcggtggtga
     5101 agccgttgaa ccacacgtcg cggtagttcg ccgaggtgcc ggtcttgccg gcgatcggcc
     5161 ggccgagctg ggcggcgaac cgggcggtgc cgcgcgccac cacgcccttc atgaggttcg
     5221 tcacgacgta gtcgacgtcg ggcgagacca cctgcggacc gggcggctgc tggcgaaggt
     5281 cctcgacgac gttgcccgcg gtgtcggtga cgaggtcgaa gaaccgcggg gtgatccggc
     5341 ggccgcccga ggcgatgccg gcgtagccgg tcgcgacctc gagcggcgtg aggtccggcg
     5401 tgcccaggct gatcgagatg tggcgcggga tcggcgaggt gatgccgtag ccgcgcagga
     5461 tctcgatgat gcgatcgagc ccgaccgaga ccgcgagctg gaccgagatc gtgttgagcg
     5521 agtaggcgag cgcggtcatc agcgtgacgt cgccgaggta cttgttgtcg tagttggcgg
     5581 gcgtccacac gccggtcgcg gtggtgaccg agaacgggcc gtcgaccagg cggtcgaccg
     5641 gggtcttgcc tgcctcgagc gcggccgagt agatgaacgg cttgatcgac gatccgacct
     5701 ggcgcttggc ctgggtcgcg cggtcgaact gcgacgcggt ccagtcgtag ccgccgacca
     5761 gcgccagcac gcggccggtg ccgggctcga tcgcgatgaa cgagccctgc agcgcggggc
     5821 gctgcgccag cacggcctgg ttgccgtcgg gcgacagccg gaccggcaac agatcgccga
     5881 gcgcgaccgg cttgccggtc ctggcgtcgc ggaacgcgcg gacgtcgcgg gcgtcggcgt
     5941 cgatcagcgc catgcgcctg gggccgaggt cgagggtgac gccggcgccg ctgcggggca
     6001 ggtcgacgac catcgcgccg tagacctgct cgggcaggac ctggtcggcg accgcggcga
     6061 cgtcgtcggt cgggccgctg atcgggtggg ccggcccgcc ggtccacgcg gcgcgcgcgg
     6121 cgggcgcgat cgcgccgatc gggccgcgga acccgagctt gcggtcgagc gcctcgaggc
     6181 cgcggcgcag cgcgctctcg gccgccgact gcatccgggt gtcgatcgtc gagtagaact
     6241 tgaggccgcc gcggaacagg tcgcggttgc cgtagcgccg ggtcgcgagc tggcggatgt
     6301 gctcgacgaa gtacggcgac gccaggtggt tgaggtcgct ctcgtcgacc agcgcgatcg
     6361 gctcggcgag cgccgcctcg tactcggcgt tggagatgta gtggtcgtcg cgcatgtggc
     6421 cgagcacgta gcgctggcgc tcgcgcgcga gctgcatgtt gcggtgcggc gcgtacttgg
     6481 tcggcgacgc gaccaggcct ccgagcatcg cggcctcggc gatcgtgagg tcctcgacct
     6541 ccttgccgaa gtaggtctgg gcggcggcgc cgacgccgta cgccccgtgg ccgaggtaga
     6601 cgtggttgag gtagatcgag aggatctcgg ccttggtcag ctcgcgctcg aggcggatcg
     6661 cgaggatgac ctccttggcc ttgcggcggt acttgcgcgc cttgcgctcc ttggcgtact
     6721 cggcgggggt caggtcgagc gcgccgatct gctcctcgcc ggcgagcagg gtctgcttga
     6781 tgatctgctg ggtgatcgtc gagccgccct gcttgatgtt gccgtcggac cggaagttgg
     6841 tgatcgcggc gcgcgcgatc ccgagcaggt cgaagccctt gtgcttgtag aaccggcggt
     6901 cctcggcgga caggaacgcg gcggggacgt gcggcggcat gcggtcgagc gggacgatcc
     6961 ggcggttctc gatcgagaac gcgccgatct cctcgccgtc ggacgacagc acgacgctct
     7021 tgcggctcgg ctggtagtcg agcagctgcg acaggtcctt gggcagctcg ttgttgatgg
     7081 tctggaccgc gaggtagatc gcggcggccg cgaacgacgc gccgtagatc gcgagcagga
     7141 agcccatgcg cagcaccgac acggtgcgca ggcgccggcg ccttcggcgc ttgcggggga
     7201 tcgcgggctc ggccacggta tgctccgagt ctatgaggtc ccggcgatgg ctgcacgcga
     7261 cgccgctggt cgcggcggtt gccgtcgcgg ccggggtcgc catcgctccc gccgcgc
//
LOCUS       DASVOR010000931         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_97518,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000931 DASVOR010000000
VERSION     DASVOR010000931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97518"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1492)
                     /locus_tag="VF516_18050"
     CDS             complement(8..1492)
                     /locus_tag="VF516_18050"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEX8109642.1"
                     /translation="MNFDTSKLASDLGYLVPYLILAVTGMVLVLAEAFYRGKDRSALM
                     SLTVAGALASAIASVVLYRQLPDAAGAKISLLGDMLVADRTGYVLSALFAVTTALAAL
                     GSPAHQREHDWQVGEYYGVMLLSTSGMVMLAHAWNLVTVFLGIETMSLGVYVMCAMRR
                     RSKRGNEAAMKYFLIGAFATGFLMYGIALIYGAAGTTKIPELALRLGTTKNPGLVVAG
                     GLMLVIAFGYKVAAVPFHMWAPDAYEGAPTPVTGFMAAAVKAAAIAAMIRLFGVALGG
                     SVVPFGALGWASPMAVVAALTITIGNVTAVRQDNIKRMLAYSSISHAGILLVGICALG
                     LGSPTATTSLVYYLIAYSFTTLGAFAIVAFVGSRQRERLLVDDWAGFGAQHPAVALAM
                     TVLLLSFGGGPPTGGFFAKFYLFKAAMEASDGQLLWLVVLGVVNSAISIYYYLRIVTA
                     MYFRDANQPFTVTRSAGLVFVIAVCPILVLELGVMPGWWLRLIG"
BASE COUNT          267 a          555 c          556 g          191 t
ORIGIN      
        1 cgcccggtca gccgatcagc cgcagccacc agcccggcat cacgccgagc tcgagcacca
       61 ggatcgggca caccgcgatc acgaacacca ggcccgccga ccgggtcacc gtgaacggct
      121 ggttggcgtc gcggaagtac atcgcggtga cgatccgcag gtagtagtag atgctgatcg
      181 ccgagttgac cacgccgagc acgaccagcc acagcagctg gccgtcgctg gcctccatcg
      241 cggccttgaa gaggtagaac ttggcgaaga acccgccggt cggcggcccg ccgccgaacg
      301 acagcagcag caccgtcatc gcgagcgcga ccgccgggtg ctgggcgccg aagccggccc
      361 agtcgtcgac cagcagccgc tcgcgctggc gcgagccgac gaacgcgacg atcgcgaacg
      421 cgcccagcgt cgtgaagctg taggcgatga ggtagtagac caggctggtg gtcgcggtcg
      481 gcgagcccag gccgagcgcg cagatcccga ccagcaggat gccggcgtgc gagatcgacg
      541 agtacgccag catgcgcttg atgttgtcct ggcgcaccgc ggtgacgttg ccgatcgtga
      601 tggtcagcgc cgcgacgacg gccatcgggc tggcccagcc gagcgcgccg aacggcacca
      661 cgctgccgcc gagcgcgacg ccgaacagcc ggatcatcgc ggcgatcgcg gcggccttga
      721 ccgccgccgc catgaagccg gtcaccgggg tcggcgcgcc ctcgtaggca tcgggtgccc
      781 acatgtggaa cggcaccgcg gcgaccttgt agccgaacgc gatcaccagc atcagcccgc
      841 cggcgaccac caggccgggg ttcttggtgg tgccgagccg cagcgcgagc tcggggatct
      901 tggtggtgcc ggccgcgccg tagatcagcg cgatgccgta catcaggaac ccggtcgcga
      961 acgcgccgat caggaagtac ttcatcgcgg cctcgttgcc gcgcttggac cgccgccgca
     1021 tcgcgcacat cacgtacacg ccgagcgaca tggtctcgat cccgaggaac acggtgacca
     1081 ggttccaggc gtgcgcgagc atcaccatgc ccgaggtcga cagcagcatc acgccgtagt
     1141 actcgccgac ctgccagtcg tgctcgcgct ggtgcgcggg cgagcccagc gcggccagcg
     1201 cggtggtcac cgcgaacagc gccgacagca cgtagcccgt gcggtcggcg accagcatgt
     1261 cgccgagcag cgagatcttg gcgccggcgg cgtcgggcag ctggcggtac agcacgaccg
     1321 aggcgatcgc cgacgcgagc gccccggcga cggtcagcga catcagcgcc gagcgatcct
     1381 tgccgcggta gaacgcctcg gcgagcacca gcaccatgcc ggtgaccgcg aggatgaggt
     1441 acgggaccag gtagcccagg tccgaggcga gtttcgaggt gtcgaagttc atggcgtggc
     1501 cctcccgccg gcgctaccgg cccccggcgc gggcgctccc ggcggtgcgg ccgagcccgc
     1561 ggcggggtt
//
LOCUS       DASVOR010000932         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_301719,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000932 DASVOR010000000
VERSION     DASVOR010000932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301719"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VF516_18055"
     CDS             <1..991
                     /locus_tag="VF516_18055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109643.1"
                     /translation="PALVFTASLGAAAAPPPAAPPAPAIDLYVAGATLDNQLLIEHVT
                     AEANVTKVTEAFRGGKPSAWQWLDAHTLVELFQDDKDETALAMIVDGQPAPQRAIRVD
                     NATWPAEAKDWPQRFALLHGGVWLIREPKPGGKSKDRPKPVFRRVDVTPNVTQREPPA
                     REIASVDAEKRAWLDGLPSVKPPAGVTVTKTRARIGRQTLGAVRCKPARGAVTTFPTA
                     AVPPFLRIGVDAVKFVSPTLPLYVVSGTAGALPDGPRFMTAAFQGCTKAALHGFAWGG
                     GDVFATIAGTPDGAGLSRQDKRTLQLWAAGRVVANMPLLGDPALSSAAPARVP"
     gene            1047..2138
                     /locus_tag="VF516_18060"
     CDS             1047..2138
                     /locus_tag="VF516_18060"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX8109644.1"
                     /translation="MLLPTTMVGSFPRPGWFTHQLAGRDILEAFKLAAHSEAFHDATR
                     VVIRDQEDAGLDILSDGQMWFDDYHMGIGSFLWYWLERTRGFSSEKLPHPAHGKARGR
                     DIWALNEAGGVAVEGEIQRGPVRMALLYRWAQHHTARPIKACIGAGPVQLSTLAYFRS
                     GPVKDRYQLSRALADVFRAEVAEVVEAGCKHIQLEDLGAWIPYLSGARDYDWVCEIVD
                     RTLGETPPGVERAWHFCMGNAWGNRLEGMTAGGYREVLSHYHGVKVDAYVLDFACREM
                     ADADLLRALPADKRVHVGVIDVRSLEIEPPAQVADRIRKVLAHIEPERVTLTTDCGLK
                     QLPRVVAVEKLRALVEGAAIVRRELSAGR"
BASE COUNT          330 a          844 c          865 g          319 t
ORIGIN      
        1 ccctgccctg gtgttcaccg cctcgctcgg cgcggccgcc gcgccgccgc ctgccgcgcc
       61 gccggcgccc gcgatcgacc tctacgtcgc cggggccacg ctcgataacc agctcctgat
      121 cgagcacgtc acggccgagg cgaacgtcac caaggtgacc gaggcgttcc gcggcggcaa
      181 gccgtcggcg tggcagtggc tcgatgcgca cacgctggtc gagctgttcc aggacgacaa
      241 ggacgagacc gccctggcga tgatcgtcga cggccagccg gcgccgcagc gcgcgatccg
      301 ggtcgataac gcgacgtggc cggcggaggc caaggactgg ccgcagcggt tcgcgctgct
      361 gcacggcggg gtgtggctga tccgcgagcc gaagccgggc ggcaagtcca aggacaggcc
      421 caagccggtg ttccggcggg tcgacgtcac gccgaacgtg acgcagcgcg agccgccggc
      481 gcgcgagatc gcctcggtcg acgccgagaa gcgcgcgtgg ctcgatggcc tgccgtcggt
      541 caagccgccc gccggggtca ccgtgacgaa gaccagggcg aggatcggcc ggcagaccct
      601 gggcgcggtg cgctgcaagc cggcgcgcgg cgcggtgacg acgttcccga ccgcggcggt
      661 gcccccgttc ctgcggatcg gcgtcgacgc ggtgaagttc gtgagcccga cgctgccgct
      721 ctacgtggtg agcgggacgg ccggcgcgct gcccgacggg ccgaggttca tgaccgccgc
      781 gttccagggc tgcaccaagg cggcgctgca cggcttcgcg tggggcggcg gcgacgtgtt
      841 cgcgaccatc gccggcacgc ccgacggcgc ggggctgtcc aggcaggaca agcgcacgct
      901 gcagctgtgg gccgccggcc gggtggtcgc gaacatgccg ctgctcggcg atccggcgct
      961 gtcgtcggcc gccccggccc gcgtgccgtg acagcggcta ttccacgcgc tgcacccggg
     1021 cgcgccaggt gtggtatccc cgcggcatgc tgctaccgac gacgatggtc gggtcgttcc
     1081 cgcggccggg gtggttcacc caccagctgg ccggacggga catcctcgag gcgttcaagc
     1141 tcgccgccca cagcgaggcg ttccacgatg cgacgcgcgt ggtgatccgc gaccaggagg
     1201 acgcgggcct cgacatcctc agcgacggcc agatgtggtt cgacgactac cacatgggga
     1261 tcggatcgtt cctgtggtac tggctggagc gcacccgcgg gttctcgtcg gagaagctgc
     1321 cgcacccggc gcacggcaag gcgcgcggcc gtgacatctg ggcgctcaac gaggccggtg
     1381 gcgtcgcggt cgagggcgag atccagcgcg gcccggtgcg catggcgctg ctctaccgct
     1441 gggcgcagca ccacaccgcg cggccgatca aggcgtgcat cggcgccggc ccggtccagc
     1501 tgtcgacgct ggcgtacttc cgctccggcc cggtcaagga ccgctaccag ctgtcgcgcg
     1561 cgctcgccga cgtgttccgc gccgaggtcg ccgaggtggt cgaggccggc tgcaagcaca
     1621 tccagctcga ggacctcggc gcctggatcc cgtacctgtc gggcgcccgc gactacgact
     1681 gggtgtgcga gatcgtcgac cgcacgctcg gcgagacccc gcccggcgtc gagcgcgcct
     1741 ggcacttctg catgggcaac gcgtggggca accggctcga gggcatgacc gcgggcggct
     1801 accgcgaggt gctgtcgcac taccacggcg tcaaggtcga cgcctacgtg ctcgacttcg
     1861 cgtgccgcga gatggccgac gccgacctct tgcgcgcgct gcccgccgac aagcgggtgc
     1921 acgtcggcgt gatcgacgtc cgctcgctgg agatcgagcc accggcgcag gtcgccgacc
     1981 ggatccgcaa ggtgctggcg cacatcgagc ccgagcgggt cacgctgacc accgactgcg
     2041 ggctcaagca gctgccgcgc gtggtggcgg tcgagaagct gcgcgcgctg gtcgagggcg
     2101 cggcgatcgt ccgccgcgag ctatcggcgg gccggtagcc ggttcgccgc gatcagcgcc
     2161 cgggccggca atcgtaccct tgtttcgtaa cgctgttacg atatagttcc caccggacct
     2221 ccaggagcgg caccgatgga atctctcgtc agctaccggc tcgatcaggc gatcgccacg
     2281 atcactctcg acgacggcaa ggtcaacgcg ctgtcgcccg ccatgcaggc gcagatcggc
     2341 ggcgcgctcg accaggcc
//
LOCUS       DASVOR010000933         2189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417861,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000933 DASVOR010000000
VERSION     DASVOR010000933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417861"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1987
                     /locus_tag="VF516_18065"
     CDS             <1..1987
                     /locus_tag="VF516_18065"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX8109645.1"
                     /translation="RLRALDSYEARRMPGVLAVLAPGTAPRLPAAEHNPRNPGDRVLQ
                     LLQDDKIWYADQPIAVVVADTLEHAQHAAARITATYDATAPVAEIESALGDAYDPGKM
                     GPNGEARSSRGDLDAGLIAAKTKITQTYTTPVENHNPMEPHATIAVWQGDTKLTLYDA
                     TQGIFGVRGKIARTFGLDPRDVRVIDHYVGGGFGCKGTPWSHVALAALAARAVGRPVK
                     LVLTRPQMFSLVGHRPKTIQTLTLGADEAGELTAIRHTVVSETSRFDEFVEPSALQTR
                     HLYACPNVVTSHKVVRIDVPTPTFQRAPGESTGTFALESAMDELAGALGIDPLELRIR
                     NHSVRDEQEDKPYSSNALLECYRRAAERFGWARRDPRPGAMRDGNTRIGWGMATATYP
                     AKMSPSSAIARMTPDGTLVVQAGTQDLGTGTYTIMTQIAADAMGVPVDRVRFELGDTA
                     FPETPVSGGSQTAASTGTAVKLAVTKLRDKLAALRTSPDEPWAAVVSRSGQPELTAEH
                     RTTERPATHSYHSHGAVFTELRIDPDSGEIRVVRVVAAYAAGTILNPRTARSQLQGGI
                     VWGIGMALQEHTVRDLRTARVVTRDLADYHVPVHADVPELDVTFVDEPDPFVNPLGVK
                     GIGELGITGITAAIANAVYHATGRRIRDLPITLDKLL"
BASE COUNT          341 a          842 c          747 g          259 t
ORIGIN      
        1 ccggctgcgc gcgctcgaca gctacgaggc gcgtcggatg ccgggcgtgc tcgcggtgct
       61 cgcgccgggc accgcgccgc gcctgccggc cgccgagcac aaccccagga acccgggcga
      121 ccgggtgctc cagctgctgc aggacgacaa gatctggtac gccgaccagc cgatcgccgt
      181 ggtcgtcgcc gacacgctgg agcacgccca gcacgccgcc gcgcggatca cggcgaccta
      241 cgacgccacc gcgccggtcg ccgagatcga gtccgcgctc ggcgacgcct acgacccggg
      301 caagatgggc cccaacggcg aggcgaggag cagccgcggc gacctcgacg ccgggctgat
      361 cgccgcgaag accaagatca cccagaccta caccacgccg gtcgagaacc acaacccgat
      421 ggagccgcac gcgacgatcg cggtgtggca gggcgacacc aagctgacgc tgtacgacgc
      481 gacccagggc atcttcggcg tgcgcggcaa gatcgcccgg acgttcgggc tcgacccgcg
      541 cgacgtccgc gtgatcgacc actacgtcgg cggcgggttc ggctgcaagg gcacgccgtg
      601 gtcgcacgtc gcgctcgcgg cgctcgccgc cagggcggtc ggccggccgg tcaagctcgt
      661 gctgacccgc ccgcagatgt tctcgctggt cggccaccgg cccaagacga tccagaccct
      721 cacgctcggg gccgacgagg ccggcgagct caccgcgatc cgccacaccg tggtgtcgga
      781 gacctcgcgg ttcgacgagt tcgtcgagcc cagcgcgctg cagacccggc acctgtacgc
      841 gtgccccaac gtggtcacct cgcacaaggt ggtgcggatc gacgtcccga ccccgacgtt
      901 ccagcgcgca ccgggagagt ccaccgggac gttcgcgctc gagtcggcga tggacgagct
      961 ggccggcgcg ctcggcatcg atccgctgga gctgcggatc cgcaaccaca gcgtgcgcga
     1021 cgagcaggag gacaagccgt actcgagcaa cgcgctgctc gagtgctacc ggcgcgccgc
     1081 cgagcggttc ggctgggccc ggcgcgatcc gcggcccgga gcgatgcgcg acggcaacac
     1141 gcggatcggc tggggcatgg cgaccgcgac ctacccggcg aagatgtcgc cgtcgtcggc
     1201 gatcgcgcgg atgacgcccg acggtaccct ggtggtccag gccggcaccc aggacctcgg
     1261 caccgggacg tacacgatca tgacccagat cgcggccgac gcgatgggcg tcccggtcga
     1321 ccgcgtccgg ttcgagctcg gcgacaccgc gttccccgag accccggtgt cgggcggctc
     1381 gcagaccgcg gcgagcaccg gcaccgcggt caagctcgcg gtcaccaagc tgcgcgacaa
     1441 gctcgcggcg ctgcggacct cgcccgacga gccgtgggcc gcggtggtgt cgcgcagcgg
     1501 ccagcccgag ctcaccgccg agcacaggac caccgagcgc ccggcgaccc actcgtacca
     1561 ctcgcacggc gcggtgttca ccgagctccg gatcgacccc gacagcggcg agatccgcgt
     1621 ggtgcgggtg gtcgcggcct acgccgccgg cacgatcctg aaccccagga ccgcgcgcag
     1681 ccagctccag ggcggcatcg tgtggggcat cggcatggcg ctgcaggagc acacggtgcg
     1741 cgacctccgc accgcgcgcg tcgtgacccg cgacctcgcc gactatcacg tcccggtcca
     1801 cgccgacgtc cccgagctcg acgtgacgtt cgtcgacgag cccgatccgt tcgtcaaccc
     1861 gctcggggtc aagggcatcg gcgagctcgg catcaccggc atcaccgccg cgatcgccaa
     1921 cgcggtctat cacgcgaccg gccgccggat ccgcgacctg ccgatcacgc tcgacaagct
     1981 gctgtgattt cgctccccgc cctgcgcgac ctgtggcatg gtccgcgctc cctgtacagg
     2041 agcacgttca tgaccactcg cgcatccacg gcgatcgccg ccgcaatctt tgcggtgatc
     2101 gccgcagcga tcgtcctccc ggccgccccc gcgttcgccg atccgcccgc caagccggcg
     2161 gcggacaaga aggcggacaa gcccgccga
//
LOCUS       DASVOR010000934         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417869,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000934 DASVOR010000000
VERSION     DASVOR010000934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417869"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..907
                     /locus_tag="VF516_18070"
     CDS             503..907
                     /locus_tag="VF516_18070"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8109646.1"
                     /translation="MSKTIVVCGYGPGISHATAERFGAEGFAVALVGRTAARLADGAK
                     RLESRGIRAAAFAHDLGEPGNAGTLASQVRERLGAISVIHWNAYSHAAGDLSTAPVAE
                     LRGGLDLAVGGLVALYQAARTDLESQPDAVRS"
     gene            1087..>2260
                     /locus_tag="VF516_18075"
     CDS             1087..>2260
                     /locus_tag="VF516_18075"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEX8109647.1"
                     /translation="MLAPRICYLDLDTFFVSVERLLDPSLRGKPVVVGGRRGGRGVVT
                     SASYEVRAFGVRSGMSIREASEHAPADAVFVPGHGEAYTDYSRRAREVVERFSPVVIA
                     ASIDEHYLDFSGCERLYRRRGDADDDATILRVVRGITHTLDAELGLPASAGIATSKPM
                     AKVASGLAKPQGVLLVAAGTEAATLAPLPVRKLPGIGPVSEARLRAVGIETLAQLVSA
                     PEALLRPIFASYTAGIRCAARGEGADDLGRERPAFREHDPHGASVGTISNERTFVETS
                     ADTAPEVLSGLCERVASRARRRGVVAGQVTLKLRYTDFHTLTRARTIAPTSADFDLHR
                     VVLELYREVRTRPLPIRLLGVALAKLRPGTAQLALFDDDDRRGDAVDRVREKFGYDA"
BASE COUNT          299 a          793 c          811 g          357 t
ORIGIN      
        1 ttcgtcggct tgtagtgggt gagcgagctg gacggtggcg ccgaggcagc gggtaccgcc
       61 gatcggagca gcagctccag cgccgccgtc gcggccacga gcattggata cgtacgcctc
      121 accatcacct gcgcccgcgc gtgtggagct gtgccttgtg ccaaccgttg cgaatggcgg
      181 gccctccggc aagcggtggg ttagctttgc caggaggtcg aatccgcccc caccctacac
      241 caggcaaatg cacgaggctg gaaccactct gggtatgcgt gaggtgggac cagcgtatgg
      301 agtgcacgcc ctcgcggcca tatcggccag atgctccagc ggttctctcg caagcaattc
      361 tcgccgcccc gtgggctgtg gatcggctcc acggtggttg tcatcgctac tcgagtcctc
      421 acgtgcgctc gtgacatacc cgtcacgtcg tgtctcgtga tacttggaga ccgttctctc
      481 ggattcgacg aaaggtgctc tcatgtccaa gaccatcgtg gtttgcggct atggccccgg
      541 catctcgcac gccaccgccg agcgattcgg cgccgagggc ttcgcggtcg cgctcgtcgg
      601 acgtactgcc gccaggctcg ccgacggggc caaacgcctc gagtcccgcg gcatccgcgc
      661 ggcggcgttc gctcacgacc tcggcgagcc gggcaacgcg gggacgctcg cgtcgcaggt
      721 ccgcgagcgg ctcggtgcga tctcggtgat ccactggaac gcgtactcgc atgcggccgg
      781 cgacctgtcg accgcgccgg tcgcggagct ccgcggcggc ctcgacctcg cggtcggcgg
      841 tctcgtcgcg ctgtaccagg ccgcgcggac cgacctcgag agccagccgg acgcagtgcg
      901 ctcgtgaagg gcacggcgtt cgactccggc catgccacgc tggagcctgc cgcggtcgcg
      961 gccgcgttct gggatctgta ccgcgcgcgc accaccgcga cgcgaacgat cccgtgatcg
     1021 gcggctcgcc ggccgatggt actggaggct tgatctgaac tgatgttcag gtctacgctt
     1081 ttccccgtgc tcgcgccgcg gatctgctac ctcgatctcg acacgttctt cgtgtcggtc
     1141 gagcggctgc tcgatccgag cctgcgcggc aagccggtcg tcgtcggtgg gcggcgcggt
     1201 ggccgcggcg tcgtgacgtc ggcgagctac gaggtgcgcg cgttcggcgt gcgctcgggc
     1261 atgtcgatcc gcgaggcctc ggagcatgcg cccgcggatg cggtgttcgt gcccgggcac
     1321 ggcgaggcgt acaccgacta ctcgcggcga gcgcgcgagg tcgtcgagcg gttcagcccg
     1381 gtcgtgatcg cggcgagcat cgacgagcac tacctcgact tctccggctg cgagcggctg
     1441 taccgccggc gcggcgacgc cgatgacgac gcgacgatcc tgcgcgtggt ccgcggcatc
     1501 acgcacacgc tcgacgcaga gctcgggctg ccggcgagcg cgggtatcgc gacgagcaag
     1561 ccgatggcca aggtcgcgag cgggctcgcg aagccgcagg gcgtgctgct cgtcgcggcg
     1621 ggcacggaag cggccacgct ggcgccgctg ccggtgcgca agctccccgg gatcggcccg
     1681 gtgtcggagg cgcggctgcg cgcggtcggg atcgagacgc tcgcgcagct cgtctcggcg
     1741 cccgaggcac tgctgcgccc gatcttcgca tcgtacaccg cggggatccg gtgcgccgcg
     1801 cgtggcgagg gcgccgacga cctcggccgc gagcggccgg cgtttcgcga gcacgatccg
     1861 cacggcgcat ccgttggcac gatctccaac gagcgcacgt tcgtcgagac cagtgccgac
     1921 accgcgcccg aggtgctgtc gggcctgtgc gagcgcgtgg cgtcgcgcgc gcggcggcgc
     1981 ggcgtcgtcg cgggccaggt cacactcaag ctgcggtaca ccgacttcca caccctcacg
     2041 cgcgcgcgga cgatcgcgcc gaccagcgcc gacttcgatc tgcatcgcgt cgtcctcgag
     2101 ctgtatcgcg aggtgcggac ccggccgctg ccgatccggc tgctcggcgt cgcgctggcg
     2161 aagctccgcc ccggcactgc gcagctcgcg ctgttcgacg acgacgaccg gcgcggcgac
     2221 gcggtcgatc gcgtgcgcga gaagttcggc tacgacgctg
//
LOCUS       DASVOR010000935         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_214003,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000935 DASVOR010000000
VERSION     DASVOR010000935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214003"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1055)
                     /locus_tag="VF516_18080"
     CDS             complement(<1..1055)
                     /locus_tag="VF516_18080"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109648.1"
                     /translation="MPSGHPAPRLGRYTLLRSLGAGGMAELFLARADGIEGFAKLVAL
                     KRILPHKATSERFVRMLLNEARLVAGLDHPNIAQVHDIGLEHGEYFFAMEYVHGQDLA
                     HVLHRAPHHQLHLENALHIAIGVCAGLHCAHIARDATGRPLDIVHRDVSPSNVLVSYQ
                     GAVKLVDFGVAKAATLVSETREGVIKGKYGYMSPEQCLGDPLDRRSDVFAVGILLWEM
                     TVGRRLYASKGELASLQRIVYADAPRPSKFDPQYPEQLEHIVMRALARDPRQRYQSAE
                     QLQIDLEQFALDHRLAVSAVSMAIEMKTLFRDQVEAWSAAERAGQSLGDHLAAAGPQA
                     PIDRDDPAEYATGDDEP"
     gene            1285..>1750
                     /locus_tag="VF516_18085"
     CDS             1285..>1750
                     /locus_tag="VF516_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109649.1"
                     /translation="MAVPRRRMSFFALMMSLILVSITVGLAYRGWSYYRLSLEDRSGH
                     PEYRLLRPSGLYGNGYGWVAAMLVVLNLSYLIRRRFGSARLGSMKTWLDVHVFTGLLA
                     ASLVSFHSAFQLRTPIASVSAVSLGTVVMTGLLGRFLYALGPGGVRERLGAAL"
BASE COUNT          239 a          598 c          623 g          290 t
ORIGIN      
        1 atcggctcgt cgtcgccggt cgcgtactcc gccgggtcgt cgcgatcgat cggcgcctgc
       61 gggcccgccg cggccaggtg atcgcccagc gactgcccgg cgcgctcggc ggcggaccac
      121 gcctcgacct gatcgcggaa cagcgtcttc atctcgatcg ccatcgacac cgcggacacc
      181 gcgagccggt gatcgagcgc gaactgctcg aggtcgatct gcagctgctc ggcgctctgg
      241 tagcgctgcc ggggatcgcg ggcgagcgcg cgcatcacga tgtgctcgag ctgctcgggg
      301 tactgcggat cgaacttcga cggccgcggc gcgtcggcgt acacgatccg ctggagcgag
      361 gccagctcgc ccttggacgc gtacagccgg cggcccaccg tcatctccca cagcaggatg
      421 ccgaccgcga acacgtcgct ccggcgatcg agcggatcgc ccaggcactg ctcgggggac
      481 atgtagccgt acttgccctt gatcacgccc tcgcgggtct ccgacaccag ggtcgccgcc
      541 ttggcgacgc cgaagtcgac cagcttgacc gcgccctggt acgacaccag cacgttcgac
      601 gggctgacgt cgcggtgcac gatgtcgagc ggccggccgg tggcgtcgcg cgcgatgtgc
      661 gcgcagtgca ggccggcgca cacgccgatc gcgatgtgca gcgcgttctc caggtggagc
      721 tggtggtgcg gcgcgcggtg caggacgtgg gcgaggtcct ggccgtggac gtactccatc
      781 gcgaagaagt actcgccatg ctcgaggccg atgtcgtgga cctgggcgat gttggggtgg
      841 tcgaggccgg cgaccagccg ggcctcgttg agcagcatgc ggacgaaccg ctcgctggtc
      901 gccttgtggg gcaggatccg cttgagcgcg accagcttgg cgaacccctc gatgccatcg
      961 gcgcgggcca ggaacagctc cgccatccct ccggccccca gcgagcgcag caacgtgtag
     1021 cgcccgaggc gcggggctgg gtggcccgac ggcatccgga gatgatgcca cgacgcgcgc
     1081 cgtcacgggc tcgatgcggc gcgacacgca catgtcgtcg taccaggttc gtgcttttcg
     1141 cggcgtgagc gtgcagtcat ttgcacagtc gagcgatcct gcgagcggcc atgcgctgtc
     1201 atcctgctgt gtcgacgatg ccttcggcgc cctccgcgcc agatcggccc ggtacgcaac
     1261 gtgcaaaatg accgtcgtcc ggagatggca gtcccacggc ggcgaatgtc gttcttcgcc
     1321 ctgatgatga gcctgatcct cgtctcgatc accgtcggcc tggcctatcg cggatggagc
     1381 tactaccggc tctcgctcga agatcgcagc ggacatcccg agtatcgcct gctgcggccc
     1441 agcggcctgt acggcaacgg ctacggctgg gtcgcggcga tgctggtggt gctcaacctg
     1501 tcgtacctga tccgccggcg gttcggcagc gcccggctgg gctcgatgaa gacctggctc
     1561 gacgtccacg tgttcaccgg gctgctcgcg gcatcgctgg tgtcgttcca ctcggcgttc
     1621 cagctccgca cgccgatcgc gtcggtcagc gcggtgagcc tggggacggt ggtgatgacc
     1681 gggctgctcg gccggttcct ctacgcgctg gggccgggcg gggtgcgcga gcggctcggt
     1741 gcggcgctcg
//
LOCUS       DASVOR010000936         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67651,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000936 DASVOR010000000
VERSION     DASVOR010000936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67651"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VF516_18090"
     CDS             <1..590
                     /locus_tag="VF516_18090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8109650.1"
                     /translation="DAWVIGTVGRIAAEKNQALLVQAMAPLLGPGTHLVIAGDGPLLP
                     ALTERVSGLGVAGFVHLLGARKDVPDLLNALDTFVLSSDTEGLPLVVPEAMATGLPVI
                     STAVGGVPSVIDDGQTGFLVPRGDEQALRDRAAALQADPAASRAMGQRARTAAVARFS
                     AERMQRDYLELYGRVLAREPWYPEPKRDVRDRRSV"
     gene            565..>1887
                     /gene="asnB"
                     /locus_tag="VF516_18095"
     CDS             565..>1887
                     /gene="asnB"
                     /locus_tag="VF516_18095"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8109651.1"
                     /translation="MCGIVGLYNFGGSTEASERDRALVLEMRDVIAHRGPDDGGLWQS
                     DDRRVVFGHRRLAIVDLSPAGHQPMSNEDGSVWITFNGEIYNHAELRAELRLDDRHQF
                     RSRSDTEVILHLYEERQIEVPQALDGMFAFALWDAKRRRLLLARDRMGKKPLYYTIAG
                     GRLLFASELKALLGHPDVARRLDPVALNEYLTFSNVPEPRTLFEGIRKLPAGHTLVCD
                     PQGNLTIARYWSPLDGPPWIAPASRGEAVERVRDLVKRAVKKRLMADVPVGAFLSGGV
                     DSSTNVALMSRLVSTPLQTFTIEFTGFGPAENFHDVPYARQVASMFGCRHTEVQVTAA
                     EALAYLPQMVQHQDEPLGDSACLPMHFVSRAARAAGLKVVLVGEGSDEVFCGYPEFPR
                     LLETYNGKWSQLRRMPQLLRRAVKRGAELAGAPSGRVDVLRRAAEDEPL"
BASE COUNT          255 a          683 c          682 g          267 t
ORIGIN      
        1 ccgacgcctg ggtgatcggc acggtggggc ggatcgccgc ggagaagaac caggcgctgc
       61 tggtccaggc gatggcgccg ctgctcggcc ccgggacgca cctggtgatc gccggcgacg
      121 ggccgctcct gccggcgctc accgagcggg tctccgggct cggcgtcgcc ggattcgtcc
      181 acctgctggg ggcgcgcaag gacgtccccg acctcctcaa tgcgctcgac accttcgtgc
      241 tgtcgtcgga caccgagggg ctgccgctcg tggtgcccga ggcgatggcg accggcctgc
      301 cggtgatctc gaccgcggtc ggcggcgtgc cgagcgtgat cgacgacggc cagaccggct
      361 tcctggtgcc ccggggggac gagcaagcgc tgcgcgatcg ggcggccgcg ctgcaggccg
      421 acccggccgc gagccgcgcc atggggcagc gcgcccggac ggccgcggtc gcgcggttct
      481 cggccgagcg gatgcagcgc gattatctgg agctctacgg tcgcgtgctg gcgcgcgaac
      541 cgtggtatcc tgagccaaag cgggatgtgc gggatcgtcg gtctgtataa cttcgggggg
      601 agcaccgaag cgtccgaacg cgaccgcgcg ctcgtcctcg agatgcgcga cgtgatcgcg
      661 caccgcggcc ccgacgacgg cggcctgtgg cagagcgacg accgccgcgt ggtgttcggc
      721 catcgccggc tggcgatcgt cgacctcagc cccgccgggc accagccgat gtccaacgag
      781 gacggctcgg tctggatcac gttcaacggc gagatctaca accacgccga gctgcgcgcc
      841 gagctccggc tcgacgatcg ccaccagttc cgatcgcgca gcgacaccga ggtgatcctc
      901 cacctctacg aggagcgcca gatcgaggtc ccgcaggcgc tcgacgggat gttcgcgttc
      961 gcgctgtggg acgccaagcg gcggcggctc ttgctggcgc gcgaccggat gggcaagaag
     1021 ccgctgtact acacgatcgc cggcggccgg ctcttgttcg cgtccgagct caaggcgctg
     1081 ctcggccacc ccgacgtcgc gcgccggctc gacccggtcg cgctcaacga gtacctgacg
     1141 ttctccaacg tccccgagcc gcgcacgctg ttcgagggca tccgcaagct gccggccggg
     1201 cacacgctgg tgtgcgaccc gcagggcaac ctgacgatcg cgcgctactg gtcgccgctc
     1261 gacggcccac cgtggatcgc gccggccagc cgcggcgagg cggtcgagcg cgtccgcgac
     1321 ctggtcaagc gcgcggtgaa gaagcggctg atggccgacg tcccggtcgg cgcgttcctg
     1381 tccggcggcg tcgactcgtc gaccaacgtc gcgctgatga gccggctggt atcgaccccg
     1441 ctgcagacct tcaccatcga gttcaccggg ttcgggcccg ccgagaactt ccacgacgtt
     1501 ccgtatgcgc gccaggtggc gtcgatgttc ggctgccgcc acaccgaggt ccaggtcacc
     1561 gcggccgagg cgctggccta cctgccccag atggtccagc accaggacga gccgctcggc
     1621 gattcggcct gcctgccgat gcacttcgtg tcgcgcgccg cgcgcgccgc cggcctcaag
     1681 gtcgtgctgg tcggcgaggg cagcgacgag gtgttctgcg gctaccccga gttcccccgg
     1741 ctgctcgaga cgtataacgg caagtggtca cagctccggc gcatgcccca gctcctgcgc
     1801 cgggcggtga agcgcggcgc cgagctcgcc ggcgcgccga gcggccgggt cgacgtgctc
     1861 cggcgcgccg ccgaggacga gccgctg
//
LOCUS       DASVOR010000937         3820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_389036,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000937 DASVOR010000000
VERSION     DASVOR010000937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3820
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389036"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1750)
                     /locus_tag="VF516_18100"
     CDS             complement(<1..1750)
                     /locus_tag="VF516_18100"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8109652.1"
                     /translation="MADPAKILVVDDDPAVVELLVEELAPHYRAVGETSARAGIERAV
                     AEDFDIVISDVEMPELRGTALLAELLERKPGQLVLMITAFGSIELAVQAVRAGACDFV
                     TKPFKIPALLFAVERALRERTMRREIVRLRRELGDDRGSLVASSPAMVKVLDVARRAS
                     RSMASVLITGESGSGKGALARWIHDRGARAAGPFVHVNCAALPSGLVEAELFGVRRGA
                     YTDARESRDGLFVEASQGTIFLDEIAEMPLDVQSKLLQVLESSRVRPVGGGEVAIDVR
                     VIAATNRAIDDAVHAGQFRLDLFFRLNVIRIEMPPLRERAGDIPELVNALLTRASRGA
                     AGPIGITEEAMRWLVRHDWPGNVRELANVVERAVALTDHDTIVLEDVRDLPSRAPADP
                     TGDLLASAAERQLPLAEVERGYIRRVLDACDGNVSRAARILGIDRRTLYRKLEERVAA
                     RRQPAAEQTAPPPRCTDMMAAIMPSVSPASTSQVAERRSQPCLGPSVETAFRDALIES
                     LDGLPLGDRNLICFHYFHGLGADQLAEMFSTHRAAVVRRLGRIRERLLRDTRRGLETR
                     LVPEGNAARGIDRPALD"
     gene            complement(1759..3219)
                     /locus_tag="VF516_18105"
     CDS             complement(1759..3219)
                     /locus_tag="VF516_18105"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8109653.1"
                     /translation="MSIRLRFTIALTLLGLLLVGGYAVFAYGWEVDDLDSATEREVLT
                     LGRSLATSLGNALRDRQRADIDEMLRALDPLEPKIFVHVHDVDGREIVRSKGDPVDAA
                     AEAAIGALARRAALTARESVAQGDRRFVYAAPLTTDSGEVIGAIVVARVTDDLRADLA
                     RTRWRLIAVVVAFVAVTLAAGVALGTAYITRPLAIVLDGIAHVRAGDFRSAVPLAHHD
                     EIGALVDEFNAMVAALADARQRIDQETEARLRLERGLQGIDKMVTIGQLSAGLAHEIG
                     SPLQVLSARAAALATRSADPETRRQAEILVEQAARITRIVDQLLSFGRRRAAVIAPRD
                     LARPVRTVLELLDGEARRKGVALELAVDAGDHTIDADEDQLQQIVLNLVRNSLAATPS
                     GGRITVRIEAVTETATEAARVRLTVRDTGPGIPPEMQARLFEPFFTTRASEGGTGLGL
                     AVVRAIVIEHGGTITARSDRGAEFIVSFPARPPGVG"
     gene            3378..>3820
                     /locus_tag="VF516_18110"
     CDS             3378..>3820
                     /locus_tag="VF516_18110"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8109654.1"
                     /translation="MWVAACSPAAGETVSFSPPTSTPRPDGTVRLAKESRQYVVTEKV
                     ALGAASPVVLAPARIAFRDGAVSQVNLPITGRITAIHVKTGDRVKAGDPLVTISSPDA
                     AAARAQLAAATAEHDAAVQETARQDTMARSGVGVDSERVTVQAHLR"
BASE COUNT          505 a         1400 c         1422 g          493 t
ORIGIN      
        1 ggtcgagcgc gggccggtcg attccacggg cggcgttgcc ctcgggcacc agccgcgtct
       61 ccaggccgcg ccgggtgtcg cgtagcaggc gctcgcggat ccggccgagc cggcgaacca
      121 cggctgcccg gtgggtggag aacatctcgg cgagctgatc ggcgccgagc ccgtggaagt
      181 agtggaagca gatcagattc cggtcgccca gcggcaggcc gtcgaggctc tcgatcagcg
      241 cgtcgcggaa cgcggtctcg acgctggggc ccaggcacgg ctggctcctg cgctcggcga
      301 cctgcgaggt cgaagccggg gacaccgagg gcatgatggc agccatcatg tcggtgcaac
      361 gcggcggcgg ggcggtttgt tccgccgccg gctggcgccg agccgccacc cgctcctcga
      421 gcttgcggta cagcgtgcgg cgatcgatgc cgaggatccg cgcggcgcgc gacacgttgc
      481 cgtcgcacgc gtcgagcacc cggcggatgt agccgcgctc gacctcggcg agcggcagct
      541 ggcgctccgc ggcgctggcc aggaggtcgc cggtcggatc ggccggcgcg cgcgacggca
      601 ggtcgcggac gtcctcgagc acgatggtgt cgtgatcggt cagcgcgacc gcgcgctcga
      661 ccacgttggc cagctcgcgc acgttgccgg gccagtcgtg gcggaccagc cagcgcatcg
      721 cctcctcggt gatgccgatc gggcctgcgg cgccgcgcga cgcccgggtg agcagcgcgt
      781 tgaccagctc ggggatgtcc ccggcgcgct cgcgcagcgg cggcatctcg atccggatca
      841 cgttgagccg gaagaacaga tcgagccgga actggccggc gtgcaccgcg tcgtcgatcg
      901 cgcggttggt cgcggcgatc acccggacgt cgatcgcgac ctcgccgccg ccgaccggcc
      961 ggacccgcga cgactccagg acctggagca gcttgctctg gacgtcgagc ggcatctcgg
     1021 cgatctcgtc gaggaagatc gtgccctgcg aggcctcgac gaacaggccg tcgcgcgact
     1081 cgcgggcgtc ggtgtacgcg ccgcgccgga ccccgaacag ctcggcctcg accaggccgc
     1141 tcggcagcgc cgcgcagttg acgtggacga acgggccggc ggcgcgcgcg ccgcgatcgt
     1201 ggatccagcg cgccagcgcg cccttgccgc tgccgctctc gccggtgatc agcacgctgg
     1261 ccatcgatcg cgacgcgcgg cgcgcgacgt cgagcacctt gaccatcgcc gggctcgacg
     1321 cgaccaggct gccgcggtcg tcgccgagct cgcggcgcag ccggacgatc tcgcgccgca
     1381 tcgtgcgctc gcgcagcgcg cgctcgaccg cgaacagcag cgccgggatc ttgaacggct
     1441 tggtcacgaa gtcgcacgcg ccggcgcgca ccgcctggac cgcgagctcg atgctgccga
     1501 acgccgtgat catcaggacc agctggcccg gcttgcgctc gagcagctcg gcgagcagcg
     1561 cggtgccgcg cagctcgggc atctcgacgt cgctgatcac gatgtcgaag tcctcggcga
     1621 ccgcgcgctc gatcccggcg cgcgccgagg tctcgccgac cgcgcggtag tgcggcgcga
     1681 gctcctcgac gagcagctcg accacggccg ggtcatcatc aacgacgagg atcttggcgg
     1741 ggtcggccat cccgatccct agcctacccc gggcggccgc gcgggaaagc tgacgatgaa
     1801 ctcggcgccg cgatccgagc gcgcggtgat cgtcccgccg tgctcgatga cgatcgctcg
     1861 cacgaccgcg agccccaggc cggtgccgcc ctcggacgcg cgggtggtga agaacggctc
     1921 gaacaggcgg gcctgcatct cgggcgggat ccccggcccg gtgtcgcgca cggtcagccg
     1981 gacccgcgcg gcctcggttg cggtctcggt caccgcctcg atccgcaccg tgatccgacc
     2041 gccgctcggc gtcgcggcca gcgagttgcg caccaggttg agcacgatct gctgcagctg
     2101 gtcctcgtcg gcgtcgatcg tgtggtcgcc ggcgtcgacc gcgagctcga gcgcgacgcc
     2161 cttgcggcgg gcctcgccgt cgagcagctc cagcacggtg cggaccggcc gggcgaggtc
     2221 gcgcggcgcg atcaccgccg cgcggcgccg gccgaacgac agcagctggt cgacgatccg
     2281 ggtgatccgc gcggcctgct cgacgaggat ctcggcctgg cggcgggtct cggggtccgc
     2341 gctgcgggtc gccagcgccg cggcgcgggc cgacagcacc tggagcgggg agccgatctc
     2401 gtgggccagc ccggccgaca gctggccgat cgtgaccatc ttgtcgatgc cctgcagccc
     2461 gcgctcgagc cggagccgcg cctcggtctc ctggtcgatc cgctggcggg cgtcggccag
     2521 cgccgcgacc atcgcgttga actcgtcgac cagcgcgccg atctcgtcgt ggtgcgccag
     2581 cgggaccgcc gaccggaagt cgccggcgcg gacgtgggcg atcccgtcga gcacgatggc
     2641 gagcggccgg gtgatgtacg cggtgccgag tgccacgccg gcggccagcg tgaccgcgac
     2701 gaacgcgacg acgacggcga tcaggcgcca gcgcgtgcgc gcgaggtcgg cgcgcaggtc
     2761 gtcggtgacg cgggccacga cgatcgcgcc gatcacctcg ccgctgtcgg tggtcagcgg
     2821 cgcggcgtac acgaagcggc ggtccccctg cgcgacgctc tcgcgcgcgg tcagcgcggc
     2881 gcgccgggcc agcgctccga tcgcagcctc ggccgcggcg tcgaccgggt cgcccttgga
     2941 gcgcacgatc tcgcggccgt cgacgtcgtg gacgtgcacg aagatcttgg gctccagcgg
     3001 gtcgagcgcg cgcagcatct cgtcgatgtc ggcgcgctgg cggtcgcgca gcgcgttgcc
     3061 gagcgaggtc gccagcgagc ggccgagggt caggacctcg cgctcggtgg cgctgtccag
     3121 gtcgtcgacc tcccagccat acgcgaacac cgcgtaaccg ccgaccagga gcagccccag
     3181 cagcgtcagc gcgatcgtga accgcagtcg aatcgacatc gctccccaca cgactgcctc
     3241 aggtgaggca aaaatgccta tcccagctcg cgttaaacaa agatgaaggc gcgggtctcg
     3301 tcggtgggcc gcgctggcac gctggcacgg cgcgtggagc ccgccgctgc gtgcacgcga
     3361 tccgatgctc gctcctggtg tgggtggcgg cctgctcccc ggcggccggc gagaccgtct
     3421 cgttctctcc gccgacctcg acgccccggc ccgacgggac cgtgcggctg gccaaggagt
     3481 cccggcagta cgtggtcacc gagaaggtcg cgctcggcgc ggcgtcgccg gtcgtgctgg
     3541 cgccggcccg gatcgcgttc cgcgacggcg cggtgtcgca ggtcaacctg ccgatcacgg
     3601 gccggatcac cgcgatccac gtcaagaccg gcgaccgggt caaggccggc gacccgctgg
     3661 tgacgatctc gtcgcctgac gccgcggccg cccgcgccca gctcgccgcc gcgaccgccg
     3721 agcacgacgc cgcggtccag gagacggcgc gccaggacac gatggcgcgg tcgggcgtcg
     3781 gcgtcgacag cgagcgggtc accgtgcagg cgcatctccg
//
LOCUS       DASVOR010000938         2279 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_535477,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000938 DASVOR010000000
VERSION     DASVOR010000938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535477"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1308)
                     /locus_tag="VF516_18115"
     CDS             complement(<1..1308)
                     /locus_tag="VF516_18115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEX8109655.1"
                     /translation="MRPIDKVLVANRGEIALRVMRTCRAMGIATVAVYSDADGAAPFV
                     RYADEAIRIGPAPARESYLVIPAILDAARQTGAAAIHPGYGFLSENARFSEAVADAGL
                     VFIGPPAGVIAELGSKRAAKRIAARAGVPTVPGYLGDDQSTQALVAAADHIGFPLLVK
                     ASAGGGGKGMRIVRQAGELAEAIDRARGEAASAFGDDTLLLERYIERPRHIEIQILGD
                     TYGHVVHLWERECSIQRRHQKVIEEAPSVALDPERRAAMGRAAVELGRAVGYIGAGTV
                     EFIADPAGAFYFLEVNTRLQVEHPVTELTCGLDLVREQIRIARGEPLGYAAAPPQRGW
                     AIEVRLCAEDPERDYLPTTGTLHAVDIPDTVRADIGVVAGSEIGIHYDSMLGKLIAHA
                     PTRRDAAQLLRRALEETWVPGLVTNREHLVRLLAHPAFLAGELD"
     gene            complement(1322..>2279)
                     /locus_tag="VF516_18120"
     CDS             complement(1322..>2279)
                     /locus_tag="VF516_18120"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8109656.1"
                     /translation="STVPVGTGDLVKKTVAEVTTQPFTVASNPEFLKEGDAINDFLKP
                     ARVILGADDPQAIDILRRLYNGMLRTSDRIQVMDLRSAELTKYAANAMLATRISFMNE
                     LAALADKLGADIEKVRKGVGSDPRIGPKFLFPGVGFGGSCFPKDLRALRDMGRASGVR
                     LGIIESTVEANERSKRAFGARVIERLGGSLAGKRIAVWGLAFKPETDDIREAPALDVI
                     GQLRAAGASIAGYDPIAADNVRALLGDAIALVDDPYAAAEGADAIVLITEWHQLRQPD
                     FVRLRKLVRTPLLFDGRNVWDPVAAHRAGFEYHGIGRPRPET"
BASE COUNT          304 a          822 c          836 g          317 t
ORIGIN      
        1 atcgagctcg ccggcgagga acgccgggtg cgcgagcagc cggaccagat gctcgcggtt
       61 ggtgaccagg ccgggcaccc aggtctcctc gagcgcgcgc cgcaacagct gcgcggcgtc
      121 gcgccgggtg ggcgcgtgcg cgatcagctt gccgagcatc gaatcgtagt ggatgccgat
      181 ctcgctgccg gcgacgacgc cgatgtcggc gcgcacggtg tccggaatgt cgaccgcgtg
      241 gagcgtgccg gtggtcggca ggtagtcgcg ctcggggtcc tcggcgcaca gccggacctc
      301 gatcgcccag ccgcgctgcg gcggcgcggc ggcgtagccg agcggctcgc cgcgcgcgat
      361 ccggatctgc tcgcgcacca ggtcgaggcc acaggtcagc tcggtgaccg ggtgctcgac
      421 ctggagccgg gtgttgacct ccaggaagta gaacgcgccc gcaggatccg cgatgaactc
      481 gacggtgccg gcgccgatat aaccgaccgc gcggccgagc tcgacggcgg cgcgccccat
      541 cgcggcccgg cgctcgggat cgagcgcgac cgacggcgcc tcctcgatca ccttctggtg
      601 gcggcgctgg atcgagcact cgcgctccca caggtggacg acgtggccgt aggtgtcgcc
      661 gaggatctgg atctcgatgt ggcgcggccg ctcgatgtag cgctcgagca gcagggtgtc
      721 gtcgccgaac gccgacgccg cctcgccgcg cgcgcgatcg atcgcctcgg cgagctcgcc
      781 ggcctgtcgc acgatccgca tgcccttgcc gccgccgccc gccgacgcct tgaccagcag
      841 cgggaacccg atgtggtcgg cggccgcgac cagcgcctgg gtcgactgat cgtcgcccag
      901 gtagcccggc acggtcggca ccccggcgcg cgccgcgatc cgcttggccg cccgcttgct
      961 gccgagctcg gcgatcacgc cggccggcgg gccgatgaac accaggccgg cgtcggcgac
     1021 cgcctcgctg aaccgcgcgt tctcggacag gaagccgtag ccggggtgga tcgcggccgc
     1081 gccggtctgc cgggcagcgt cgaggatcgc ggggatcacg aggtaggact cgcgggccgg
     1141 cgccggcccg atccggatcg cctcgtccgc gtaccggacg aacggagcag cgccgtcggc
     1201 gtcggagtac accgcgaccg tcgcgatccc catggcgcgg caggtccgca tcacgcgcag
     1261 cgcgatctcg ccgcggttgg cgacgaggac tttgtcgatc gggcgcatcg gcccgaagct
     1321 actacgtttc cggccgcggg cgcccgatcc cgtggtactc gaagccggcg cggtgcgccg
     1381 cgaccggatc ccacacgttg cggccgtcga acaagagcgg ggtccgcacc agcttgcgca
     1441 gccgcacgaa gtcgggctgg cgcagctggt gccactcggt gatcagcacg atcgcgtcgg
     1501 cgccctcggc cgcggcgtac gggtcgtcga ccagcgcgat cgcgtcgccg agcagcgcgc
     1561 gcacgttgtc ggcggcgatc ggatcgtagc ccgcgatcga cgcgccggcc gcgcgcagct
     1621 ggccgatcac gtcgagcgcc ggcgcctcgc ggatgtcgtc ggtctcgggc ttgaacgcca
     1681 ggccccacac cgcgatccgc ttgccggcga gcgagccgcc gagccgctcg atcacccggg
     1741 cgccgaacgc ccgcttcgag cgctcgttgg cctcgaccgt cgactcgatg atgccgagcc
     1801 ggacgccgct ggcgcgcccc atgtcgcgca gcgcgcgcag gtccttgggg aagcacgacc
     1861 cgccgaaccc gaccccgggg aacaggaact tcggcccgat ccggggatcg ctgccgacgc
     1921 ccttgcgcac cttctcgatg tcggcgccga gcttgtcggc gagcgcggcg agctcgttca
     1981 tgaacgagat ccgggtcgcg agcatcgcgt tggcggcgta cttggtgagc tccgcggacc
     2041 gcaggtccat gacctggatc cggtcgctgg tccgcagcat gccattgtac agccggcgca
     2101 ggatgtcgat cgcctgcggg tcgtcggcgc cgaggatcac gcgcgccggc ttgaggaagt
     2161 cgttgatcgc gtcgccctcc ttgaggaact cggggttcga cgcgaccgtg aacggctggg
     2221 tggtgacctc ggcgacggtc ttcttcacca ggtcgccggt gccgaccggg accgtgctc
//
LOCUS       DASVOR010000939         4322 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_272106,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000939 DASVOR010000000
VERSION     DASVOR010000939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4322
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272106"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..891)
                     /locus_tag="VF516_18125"
     CDS             complement(22..891)
                     /locus_tag="VF516_18125"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX8109657.1"
                     /translation="MADGLIELGHASEGFRVARTLEVSKSRGSAHLGGKHTFEISRAG
                     IIVHPRTEARFATPAVSEQPHGRLEFGIEQLDVMLRNGVMRGSTTGILGAPGAGKTIF
                     GLQFLAAGAQRNEPALYFGFYETPPRLIAKAQGIGIPFDKYCRSGLVDLIWQPPLEHS
                     IDGLAERLLDAAERHHVQRVFIDSVDGFRLSAMTPERLTRFFVALTNELRARGVTTLL
                     SEEIDLVGATLPTHSASLSPNFENIIVLRYVEVSRLITSRRAPRGRTNLQRRQRISPC
                     RAGGLANRAPRCS"
     gene            complement(876..1358)
                     /locus_tag="VF516_18130"
     CDS             complement(876..1358)
                     /locus_tag="VF516_18130"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RAD55 family ATPase"
                     /protein_id="HEX8109658.1"
                     /translation="MSTGIRGLDTILGGGLIARRLYVVKGPPGAGKSVLSAQLSFHRA
                     RAGEQVVYVTMLTESHSSLLENLTTLEFFSAALAADRITFVSGFRALDEGGLHGLYKL
                     MRGMIQKTGSRLLVLDGIVGTTDRMPSPEGLRRFVRNIATVSTRSTRGCRSSRTWPTA
                     "
     gene            complement(1509..1646)
                     /locus_tag="VF516_18135"
     CDS             complement(1509..1646)
                     /locus_tag="VF516_18135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109659.1"
                     /translation="MNAFAVLDRFFRGGHDRRDGVAHPQQPVLQLESHDLLVLDHQDL
                     G"
     gene            1817..2458
                     /locus_tag="VF516_18140"
     CDS             1817..2458
                     /locus_tag="VF516_18140"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX8109660.1"
                     /translation="MAFVEELGRLVSTAIDNARLYEAAQHAVRVRDNVLAVVSHDLRN
                     PLLSVSLRAEQLADYPEELSRDKVIKHGKSIRQNVERMKRLIDDLIDVARVDSGHLSI
                     EKQRHEVSEILSEIASSFEIAASQRSIALDIENARVPIVVPCDRDRILQVLSNLVGNA
                     LKFSADGTRIHVVFGSAPECEFTLFRPPVRHLISSANYDRACKRGQSRNATPR"
     gene            2701..4161
                     /locus_tag="VF516_18145"
     CDS             2701..4161
                     /locus_tag="VF516_18145"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8109661.1"
                     /translation="MSILESSTSNSSPSTTGDDAAIAELHAAFKAQRAAFHADRQPSL
                     DERRARIGRLIEMLLGNRERISAALSADFGCHPVPASDLIEVLGPVGRAKYVLEHLDD
                     WMRPSPREVDPAVMGSATAYIQYQPKGVVGNIVPWNFPFDLSVGPMIEMLAAGNRVMV
                     KPSEFTPASAALLRDMVSRAFDPTLVYVATGGLDLARAFSELPWDHLLYTGSTNVGRQ
                     VMQAAARNLTPVTLELGGKCPAVLTPGSVTATNVEHVIGTKTIKNGQMCISVDYCLVP
                     RGELGRFVDLAVEFVQRVASDYSRSPDCTGIISPRHLDRLCEMLQEARDRQCTIVELE
                     RDGRVDRATRRMPMALVVDPPPDLRIMQEEIFGPILPVVPYDRVDAAIERINAGDRPL
                     GLYVFGDDRAVVDHVLGNVTSGGAAVNACAAQGALPSLAFGGTGASGMGRHHGLEGFR
                     EFSNPRGVVVRGTGDLFDAFSAPYAKAAALVKAALG"
BASE COUNT          695 a         1455 c         1466 g          706 t
ORIGIN      
        1 tcgccggtct cggcggtcac atcaagaaca acggggcgcc cggttggcaa gtcctccagc
       61 gcggcatgga gaaattcgtt gccttcgctg caggttggtg cgcccgcgag gagctcgccg
      121 acttgtgatc aatcgagaga cctcgacgta tcgcagcacg atgatgttct cgaagttcgg
      181 cgacaggctc gcggagtgag tcggcagggt cgcgcccacc agatcgatct cctcggacag
      241 cagcgtggtc acacctcgcg cccgcagctc gttcgtgagc gcgacgaaga aacgagtcaa
      301 gcgctccggg gtcatcgccg acagccggaa cccatccaca ctgtcgatga acacgcgctg
      361 cacgtggtgg cgctcggctg cgtccagcaa gcgctccgcg agcccgtcga tcgagtgctc
      421 gagcgggggc tgccagatca gatcgacgag cccgctccgg cagtacttgt cgaacgggat
      481 cccgatgcct tgcgcctttg cgatcaggcg cggtggagtc tcgtagaagc cgaagtacag
      541 ggcgggttcg ttgcgctggg cgcctgccgc caggaattgc aagccgaaga tcgtcttgcc
      601 cgcgccgggc gcacccagga tcccggtcgt ggatccgcgc atcaccccat tccggagcat
      661 gacatcgagc tgctcgatgc cgaactccag gcgtccgtgc ggctgctcgg agaccgccgg
      721 ggtcgcgaag cgggcctcgg tcctcggatg cacgatgatt cccgcgcgcg agatctcgaa
      781 cgtgtgcttg cccccgaggt gagcgctccc gcgagacttc gagacctcca gcgtgcgtgc
      841 gacgcggaag ccctcgctcg catggcccag ctcgatcagg ccgtcggcca cgttctcgac
      901 gatcggcaac cacgggttga ccgcgtcgat accgtcgcga tgttgcgcac gaaccggcgc
      961 aacccctcgg gcgacggcat gcgatccgtt gtgcccacga tgccatccag caccaggagc
     1021 cgcgagccgg tcttctggat catccctcgc atcagcttgt acaggccgtg gagcccgcct
     1081 tcgtcgagcg cccggaagcc gctgacgaac gtgatccggt ccgcggcgag cgctgcgctg
     1141 aagaactcca gcgtcgtcag gttttccagc agggacgagt gagactcggt cagcatcgtc
     1201 acgtagacca cctgctcccc ggcgcgcgcg cggtggaacg agagctgcgc ggagagcacc
     1261 gacttgcccg ctcccggagg cccttttacc acgtacagcc tgcgagcgat cagccctccg
     1321 ccaaggatcg tgtcgagacc gcggatgccg gtggacatgc gcggcagctc ccgcgggtct
     1381 tcgggctcgg tgatgtcctt cggatttcca tggaactcac gatcagcagg atccagccca
     1441 gaggtagcgc gggtatcggg gctggtaacg gagccgtggc ttcagagcgt gccggcatgg
     1501 tgccagagtc agcccagatc ctggtggtcg aggacgagca gatcgtggct ctcgagctgc
     1561 aggacaggct gctgcggatg ggccacgccg tcccggcggt cgtggccacc gcggaagaag
     1621 cggtcgagca cagcgaacgc attcaccccg atcgcgtgac cgagctgccc gcgagcgcga
     1681 gctctccctg gcggagggtc ccgaaccagc agctcacggc acgggatgct tcccggcggc
     1741 cgatcgagat cgaatcggcc ccgatccggg actcccagga gcgaaggcgc cggcccggcg
     1801 cctcgtggag caagacgtgg ctttcgtcga ggagctcggc cgcctggtct ccaccgcgat
     1861 cgacaacgcc cgcctgtacg aagcggcgca gcacgccgtc agggtgcgcg acaacgtgct
     1921 cgcggtcgtg tcgcacgatc tgaggaaccc gctgctgtcg gtctcgttgc gcgccgagca
     1981 actggcggat tacccggagg agctgtctcg ggacaaggtg atcaagcacg ggaagagcat
     2041 ccgccagaat gtggaacgga tgaagcggct gatcgacgac ttgatcgacg tggcccgagt
     2101 tgactctgga catctctcga tcgaaaagca acgtcatgag gtgagcgaga ttctttcgga
     2161 gatcgcttca tccttcgaga tcgcggcgtc acagcgttcc atcgccctgg acatcgagaa
     2221 cgcgcgggta ccgatcgtgg tcccttgcga tcgcgatcga atcctccagg tcctatccaa
     2281 tctcgtgggg aacgcgctca agttcagcgc ggacggaacg aggattcatg ttgtcttcgg
     2341 gtccgcgcct gagtgcgaat tcacgctgtt caggcctccg gtgcggcatc tgatctcgag
     2401 cgcgaactat gatagggcct gcaaacgcgg gcaatcgagg aacgccacac cacgctagat
     2461 cgataacaac aatgcgccgt gcccggccgg attctgatcg ggccggttct ggagcggagg
     2521 aacgctgatc gattccacgg ggtcgttcgc gatccgtgcg cctgctggac agctccggga
     2581 cctggatggt cggaggcgac tccggtatgt tccgtcatga cgacggccgc gtcgtccggc
     2641 gacgtgccgg cgcacgctgt tcggccggcg tagctgccac cacgtgatcg ggaggtaccc
     2701 atgtccatcc tggagagctc gacctcgaac tcgtcaccgt ccaccaccgg cgacgacgcg
     2761 gcgatcgcgg agctgcacgc cgcgttcaag gcccagcgcg ccgcgttcca tgccgaccgc
     2821 cagcccagcc tcgacgagcg ccgggcgcgg atcggccggc tgatcgagat gctgctcggc
     2881 aaccgcgagc ggatcagcgc cgcgctgagc gccgacttcg gctgccatcc ggtgccggcg
     2941 tccgacctga tcgaggtcct ggggccggtc ggccgcgcca agtacgtgct cgaacacctc
     3001 gacgactgga tgcggcccag cccgcgcgag gtcgatcccg cggtgatggg cagcgcgacg
     3061 gcctacatcc agtaccagcc gaagggcgtc gtcggcaaca tcgtgccgtg gaacttcccg
     3121 tttgatctct cggtcggtcc catgatcgag atgctcgcgg cggggaaccg cgtgatggtc
     3181 aagccgtccg agttcacacc ggcgtcggcc gcgctgctcc gcgacatggt gtcccgtgcg
     3241 ttcgatccga cgctggtcta cgtcgccacg ggcggcctgg atctcgcgcg ggcgttctcg
     3301 gagctgccgt gggatcacct gctctacacc ggcagcacga acgtcggccg ccaggtcatg
     3361 caggccgcgg cgcgcaacct gacgccggtg acgctcgagc tcggcggcaa gtgcccggcg
     3421 gtgttgaccc cgggctcggt caccgcgacc aacgtcgagc acgtcatcgg caccaagacg
     3481 atcaagaacg gccagatgtg catctcggtc gactactgcc tcgtgccgcg cggcgagctc
     3541 ggccggttcg tcgacctggc ggtcgagttc gtgcagcggg tcgcgtccga ctactcgcgc
     3601 agccccgatt gcaccggcat catctcaccg cgccacctcg accggctgtg cgagatgctg
     3661 caggaggccc gcgatcgcca gtgcacgatc gtcgagctgg agcgcgacgg ccgggtcgac
     3721 cgcgcgaccc gccgcatgcc gatggcgctg gtcgtcgatc cgccgcccga tctgcgcatc
     3781 atgcaggagg agatcttcgg gccgatcctg ccggtcgtgc catacgaccg cgtggacgcc
     3841 gcgatcgagc ggatcaacgc aggcgaccgg ccgctcggcc tgtacgtgtt cggcgacgat
     3901 cgcgcggtcg tcgatcacgt gctgggcaac gtcacgtcgg gcggcgccgc ggtcaacgcc
     3961 tgcgccgcgc agggcgcgct gccctcgctg gcgttcggag ggaccggcgc gagcggcatg
     4021 ggccgccatc acggcctcga aggcttccgc gagttctcca atccgcgcgg tgtcgtggtg
     4081 cgcggaaccg gagatctttt cgatgcgttc tccgcgccgt acgcgaaggc ggccgcgctg
     4141 gtgaaggccg cgctgggctg accgccggcc cggcgcgcgt gccgccggag ggcgcggctc
     4201 ctcaccgggg tgccctgacg atccgcgccg gtcacggctt cttcttcggc ggtgcggaag
     4261 ccatcttctg cttgagcgcc gcgagctcgt cgtcgagcgt ggtcgccgcg ggcttcttgc
     4321 cg
//
LOCUS       DASVOR010000940         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417956,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000940 DASVOR010000000
VERSION     DASVOR010000940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417956"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..1558
                     /locus_tag="VF516_18150"
     CDS             335..1558
                     /locus_tag="VF516_18150"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8109662.1"
                     /translation="MADAPYDPFDDFNRFHALGVVRDPYPRLAELRCEGSIHKGTLRG
                     MCGQPELPWETGNTYIVVAHDAATRVLLDGKTFSSAVYARSLGRVFGHSIIEMDEPEH
                     SRYRSLIQRAFSKRALDRWEHELVRPIVSRMIDRFATRGRADLVRELTFPFPVSVIAG
                     MIGVDEADLPAFHRMATEALSVTADPERGIRASQGLRELFAHTLPERRAAPRDDLMSV
                     LAAAELDGTRLDDEAIYAFLRLLAPAGAETTYRSLSNLLFGLLRHPDQLDALRADRAL
                     MPQAIEEGLRWEPPLLGIPRRTTREVEIDGVVVPAGATVSVNLGAANRDEARFPEPDA
                     FDIFRPQKTHVAFATGAHRCLGLHLARMESTVALNALLDRLSNLRLDPAAEDVHISGL
                     TFRSPLALPVLFDPS"
     gene            1713..2177
                     /locus_tag="VF516_18155"
     CDS             1713..2177
                     /locus_tag="VF516_18155"
                     /inference="COORDINATES: protein motif:HMM:NF018430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HEX8109663.1"
                     /translation="MSFRITGLSPELFQPLFALSDAELAACGAIRRIADRKPGFPCRI
                     SLTDPEPGQEVILTHFEHHAVASPFRASHAIYVRRGEQRYDAVDQVPEQLRLRLLSVR
                     AFDRAGMLLDADVVDGPALEPVIARLLGDPRADYLHVHFAKPGCYAARIDRA"
     gene            2307..2996
                     /locus_tag="VF516_18160"
     CDS             2307..2996
                     /locus_tag="VF516_18160"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8109664.1"
                     /translation="MSKTIVVCGYGPGISHATAERFGTEGFAVALVGRTAARLADGAK
                     RLEARGIRAAAFVHDLGEPGNAGALASQIHERLGAVSVIHWNAYSRAAGDLSTAPVAE
                     LRGGLDLAVGGLVALYQAARTDLESQPDAAILITCGGLAFNDPQVDAMAVKWSAMGLA
                     IAKAAQHKLAAVLHHHLKPRGIFVGEVVVNALVKGTAFDSGHATLEPAAVAAAFWDLY
                     RARTTATQMIP"
BASE COUNT          481 a         1172 c         1166 g          550 t
ORIGIN      
        1 ttcgtcggct tgtagtgggt gagcgagctg gacggtggcg ccgaggcagc gggtaccgcc
       61 gatcggagca gcagctccag cgccgccgtc gcggccacga gcattggata cgtacgcctc
      121 accatcacct gcgcccgcgc gaatggagct gtgccttgtg ccaaacgttg cgaatggcgg
      181 gccctccggc aagcggtggg ttcgcctttg ccaggaggtg gaatccgccc ccaccctaca
      241 ccaggcaaaa tgcacgaggg tggaaccact ccgggtatgc gtgacctggg accagcgtat
      301 ggagtgcacg cccgtccatc tcacggaggt ccccgtggcc gatgccccgt acgatccgtt
      361 cgatgacttc aatcgcttcc atgcgctcgg cgtcgtgcgc gatccgtatc cgcggctggc
      421 ggagcttcgc tgcgagggca gcatccacaa ggggacgctg cgcgggatgt gcggccagcc
      481 ggagctgccc tgggagacgg gcaacaccta catcgtcgtc gcgcacgacg cggcgacccg
      541 cgtactgctc gacggcaaga cgttctcgtc ggccgtgtac gcgcggtcgc tgggccgcgt
      601 gttcggccac agcatcatcg agatggacga gcccgagcac agccgttacc gcagcctgat
      661 ccagagggcg ttcagcaagc gcgcgctcga tcgctgggaa cacgagctcg tgcggccgat
      721 cgtgagccgc atgatcgatc gcttcgccac gcgtggccgc gccgatctgg tccgcgagct
      781 gaccttcccg tttccggtgt cggtgatcgc ggggatgatc ggcgtcgacg aggcggatct
      841 cccggccttt catcggatgg cgaccgaggc gctcagcgtc accgcggatc cggagcgcgg
      901 gatccgcgca tcgcaggggc tccgcgagct gttcgcgcac acgctgcccg agcggcgcgc
      961 ggcgccgcgc gacgatctga tgagcgtcct cgcggccgcg gagctcgacg gcacccggct
     1021 cgacgacgag gcgatctacg cgttcctgcg gctgctcgca ccggccggcg ccgagaccac
     1081 ctaccgctcg ctcagcaacc tgctgttcgg tctgctccgg caccccgacc agctcgacgc
     1141 gctgcgcgcc gatcgcgcgc tgatgccgca ggcgatcgag gagggcctgc gctgggagcc
     1201 gccgctgctc ggcatcccgc gcaggaccac gcgcgaggtc gagatcgacg gcgtcgtggt
     1261 gccggcgggt gcgacggtca gcgtgaacct cggcgcggcg aatcgcgacg aggcgcgctt
     1321 cccggagccg gacgcgttcg acatcttccg gccgcagaag acccacgtgg cgttcgcgac
     1381 cggcgcgcac cgctgcctcg gcctgcacct cgcccggatg gagtcgaccg tggcgctgaa
     1441 cgcactgctc gaccgcctct cgaacctgcg gctcgatccg gcggcggagg acgtccacat
     1501 ctccgggctg acgttccgct cgccgctcgc cctcccggtc ctgttcgatc cgagctagcg
     1561 cggacttgcg cgactaccat gaccaccgtc tcggcgtgcg cgttctccga gaccgcggcg
     1621 ggtggcgcgg aactgaattg ccgagcaagc agcggagtgt gcatggcgtg gcgacggcgc
     1681 acagtgcgag tcaacccacc aggagactcg ccatgagctt tcgcatcacc gggctctcgc
     1741 ccgagctgtt ccagcccctg ttcgcgctgt ccgacgccga gctggcggcg tgcggcgcga
     1801 ttcgccggat cgccgatcgc aagcccggct ttccgtgccg gatcagcctg accgaccccg
     1861 agccggggca ggaggtgatc ctcacccact tcgagcacca cgcggtggcg tcgccgttcc
     1921 gcgccagcca tgcgatctac gtccgtcggg gcgagcagcg ctacgacgcg gtcgaccagg
     1981 tgcccgagca gctgcggctg aggctgctgt cggtgcgcgc cttcgatcgc gcgggcatgt
     2041 tgctcgacgc cgacgtcgtc gatggcccgg cgctcgagcc ggtgatcgcg cggctgctcg
     2101 gcgatccgcg ggccgactac ctgcacgtcc acttcgctaa gcccggctgc tacgccgcgc
     2161 ggatcgatcg cgcgtagctg ccagccgttc acctcgcgcc gtccctccgc ggttgcggta
     2221 cggcgccgcc gcgagctggg ccagcgtgcg cgcgaacgcg gtatatttgc agacggttgg
     2281 tctcgaattg aacgaaaggt gctttcatgt ccaagaccat cgtggtttgc ggctatggcc
     2341 ctgggatctc gcacgccacc gccgagcggt tcggcaccga gggcttcgcg gtcgccctcg
     2401 tggggcgtac cgcggccagg ctcgccgacg gggcgaagcg cctcgaggcg cgtggcatcc
     2461 gcgcggcggc gttcgtccac gacctcggcg agccgggcaa cgcgggggcg ctggcgtcgc
     2521 agatccacga gcgactcggc gcggtctcgg tgattcactg gaacgcatac tcgcgtgcgg
     2581 ccggcgacct gtcgacggcg ccggtcgcgg agctccgcgg cggcctcgac ctcgcggtcg
     2641 gtgggctcgt cgcgctgtac caggctgcgc ggaccgacct ggagagccag ccagacgcgg
     2701 cgatcctgat cacctgcggt ggtctggcgt tcaacgaccc gcaagtggat gccatggcgg
     2761 tgaagtggtc ggcgatgggg ctcgcgatcg ccaaggccgc gcagcacaag ctcgccgcgg
     2821 tgctccatca ccatctgaag ccacgcggca tcttcgtcgg tgaggtggtg gtcaatgcgc
     2881 tggtgaaagg cacggcgttc gactccggcc atgccacgct cgagcctgcc gcggtggctg
     2941 ccgcgttctg ggatctgtac cgcgcgcgca ccacggcgac gcaaatgatc ccgtgacagc
     3001 gggcgtctcg tgggcagccg ggttgccggc gcgccggaga tgaggatgtg cgacgtgcgc
     3061 ccctccggtg tggcgtcgag atcccgctcg tggcatctgc tgtgacgacc gaccgcgcaa
     3121 gctggagctc acctctggat tcgggtcaca gcggcaagcc cgatcacgga gcgctcgcca
     3181 cggcgataca ctcccctccc ggtcctgttc gaccccggct agactcgtcg caggcgactc
     3241 agtcatccgc tgcgtgttgc tgactgatgg ccatctgaat catcacctgc atcagcgcat
     3301 cctgaagttt ggccgtgtgc tgagacagtt gtggctgatg cttggtccac cactcggagt
     3361 atctttcct
//
LOCUS       DASVOR010000941         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_155647,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000941 DASVOR010000000
VERSION     DASVOR010000941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155647"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VF516_18165"
     CDS             <1..773
                     /locus_tag="VF516_18165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109665.1"
                     /translation="ALGPATRARLEQVAARAGRPDVFFTSAWGSTETSPLATSAHFVT
                     PTTGNLGLPIPGVQLKLAPVQDRLELRVRGPNITPGTWQPGGGIAPVALDGDGFLPTG
                     DAGRLVDEANPAAGIVFGGRLGENFKLSSGTWVSVAHVRLALLEACAPLVLEAVIAGH
                     DRDQLGALVFPSPSARQLAPAAVADRLRAALATYNAAHPHTSERIARALLISEPLSLD
                     DGETTDKGYTNQRRVLERRAAAVVRLFAQPPDADVLAL"
     gene            871..1065
                     /locus_tag="VF516_18170"
     CDS             871..1065
                     /locus_tag="VF516_18170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109666.1"
                     /translation="MAAFNGVKIFSATMFADRGQLGERITAWMASGPPRKLTDIVVTQ
                     SSDAAFHCVTFTIFYQEALR"
     gene            1532..>2701
                     /locus_tag="VF516_18175"
     CDS             1532..>2701
                     /locus_tag="VF516_18175"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109667.1"
                     /translation="MRNLDRSGTREREAVISLSTNSSPGELSGKFRFIAEIGHGGMSE
                     VYLALAQGGLGGFQKLVAIKLLRRDLAKDEDFRRMFLEEARLTARLNHPNVVQTNDVG
                     EDQGRYYIAMEYLEGQTFERVRRARNAGKLFPLNMQIQMLVQALSGLHYAHELTDYDN
                     TALSIVHRDVTPSNLFITYDGQVKLVDFGIAKVFDSLNHTRVGVLKGKARYMAPEQIT
                     GVTIDRRADIFSVGVMLWEMLSGAQIWPGKSETEILRRVSAGDVPLPPRDAPFVLQQI
                     CTKAMAFHADDRYPTSEALRAEPDAYLAQHMTPTRMNGLGPAVVELFREERAQIRQVI
                     ESQMRGTRSGGTALPALDEVRVKQAGESFGSFGGTPTGANPRSSQGMQLTYGGEPA"
BASE COUNT          429 a          966 c          922 g          384 t
ORIGIN      
        1 ccgcgctcgg cccggcgacc cgggcgcggc tggagcaggt cgcggcgcgc gccggccggc
       61 ccgacgtgtt cttcacctcg gcgtggggct cgaccgagac ctcgccgctg gcgacctcgg
      121 cgcacttcgt caccccgacc accggcaacc tcggcctgcc gatcccgggc gtccagctca
      181 agctcgcgcc ggtgcaggac cggctggagc tccgggtccg cggccccaac atcacacccg
      241 gcacctggca gcccggcggc ggcatcgcgc cggtcgcgct cgacggcgac ggcttcctgc
      301 cgaccggcga cgcgggccgg ctggtcgacg aggccaaccc ggccgccggg atcgtgttcg
      361 gcgggcggct cggcgagaac ttcaagctat cgtccggcac ctgggtcagc gtcgcccacg
      421 tccggctcgc gctgctcgag gcgtgcgcgc cgctcgtgct ggaggcggtg atcgccgggc
      481 acgatcgcga tcagctcggc gcgctggtgt tcccgtcgcc gtcggcgcgc cagcttgcgc
      541 ccgctgccgt cgccgatcgc ctgcgcgccg cgctcgcgac ctacaacgcc gcgcacccgc
      601 acaccagcga gcggatcgcc cgcgccctgc tgatcagcga gccgctgtcg ctcgacgacg
      661 gcgagaccac cgacaagggc tacaccaacc agcgccgggt gctggagcgc cgcgccgccg
      721 cggtcgtccg gctgttcgcg cagccgcccg acgccgacgt gctggcgctg tgaccgcagg
      781 ggtctcgggc aaaccacgag cggatgtgcg ggcgaccgcc atcgctgccg aatcggcgcc
      841 tccgccccgg ccggcgtgtt acggtgaacc gtggcagcgt tcaacggtgt aaagatcttc
      901 tcggcgacga tgttcgccga ccgcggacag ctaggcgagc ggatcacggc gtggatggcg
      961 agcggccccc cgcgcaagct gaccgacatc gtggtcacgc agtcgagcga cgcggcgttc
     1021 cactgcgtga cgttcacgat cttctatcag gaggcgctgc gctgagccgg cgagtcttcg
     1081 agccggcgat ctccgtatag gcgcagccgg caacatgccg gcaagccgat cgacaagagc
     1141 cgccggctgt ccgcggtgcg ccctgcgtac ccggatcaca gcgatcacat cggaagtaag
     1201 atcaccgtga gccggcgcga ccgaccggtg gctccgcctc acagcggggc gtgaatcgcc
     1261 cccgcgcgtc ggcgcggccg tggctgcagc tcgttcactc cacgcccgca gcggcggcca
     1321 gatccctgga acaccagcac gtgcacattc cgcttatgcg gtgcagcagc catgtcaggt
     1381 gacgtgggcc acgccgtcgc cgtcggtagc aggtcatcac tcagcccgac cgagttgtga
     1441 aaaactaccg cttgacggaa ttctcgcagc atggcctcat agtcaatgat cgacttgtaa
     1501 agaaccgtac aggcctgatg tacagggtac tgtgcggaat ctagaccggt cgggcacccg
     1561 agagagagag gccgttatct cgctatccac caacagcagc ccgggagagc tgtctggaaa
     1621 gttccggttc atcgccgaga tcgggcatgg cgggatgtca gaggtgtacc tggcgctcgc
     1681 ccagggcggc ctgggtgggt ttcagaagct ggtcgcgatc aagctgctgc gccgcgacct
     1741 ggccaaggac gaggacttcc ggcggatgtt cctggaggag gctcgcctga cggcgcggct
     1801 gaaccacccc aacgtggtcc agaccaacga cgtcggcgag gaccagggcc ggtactacat
     1861 cgcgatggag tacctggagg gccagacgtt cgagcgcgtc cgccgggcgc gcaacgccgg
     1921 caagctgttt ccgctcaaca tgcagatcca gatgctggtc caggcgctgt ccggcctgca
     1981 ctacgcgcac gagctcaccg actacgacaa cacggcgctg tcgatcgtgc atcgcgacgt
     2041 cacgccgagc aacctcttca tcacctacga cggccaggtg aagctcgtcg acttcgggat
     2101 cgcgaaggtc ttcgattcgc tcaatcacac ccgcgtcggg gtgctcaagg gcaaggcgcg
     2161 ctacatggcg ccggagcaga tcaccggcgt cacgatcgac cggcgcgccg acatcttctc
     2221 ggtcggcgtc atgctgtggg agatgctgag cggtgcgcag atctggcccg gcaagagcga
     2281 gaccgagatc ctgcgccggg tgtcggccgg cgacgtcccg ctgccgccgc gcgacgcgcc
     2341 gttcgtgctc cagcagatct gcaccaaggc gatggcgttc catgccgacg accgctaccc
     2401 gacctccgag gcgctgcgcg ccgagcccga cgcgtacctc gcccagcaca tgacgccgac
     2461 ccggatgaac gggctcggcc ccgcggtcgt cgagctgttc cgcgaggagc gggcccagat
     2521 ccgccaggtc atcgagtccc agatgcgcgg cacgcggtcc ggcggcacgg cgctgccggc
     2581 gctcgacgag gtccgcgtca agcaggccgg cgagagcttc ggctcgttcg gcggcacgcc
     2641 gaccggcgcc aacccccgga gcagccaggg catgcagctg acctacggcg gcgagccggc
     2701 g
//
LOCUS       DASVOR010000942         9333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_301812,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000942 DASVOR010000000
VERSION     DASVOR010000942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301812"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="VF516_18180"
     CDS             complement(<1..222)
                     /locus_tag="VF516_18180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8109668.1"
                     /translation="MPMGCDILVVDDIPANLVAIEAALEPLSQRIVKATSGTEALRRL
                     LESDFAVVLLDVQMPEMDGYETARWIRSRD"
     gene            complement(326..493)
                     /locus_tag="VF516_18185"
     CDS             complement(326..493)
                     /locus_tag="VF516_18185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109669.1"
                     /translation="MNSPGIAEPDVLQDVVDVGRADPPGEERPELRRDVSAGEMCAIA
                     QRALAHAWMFA"
     gene            complement(493..861)
                     /locus_tag="VF516_18190"
     CDS             complement(493..861)
                     /locus_tag="VF516_18190"
                     /inference="COORDINATES: protein motif:HMM:NF045455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109670.1"
                     /translation="MDLTFYDAAGKAVAYCDDERHVYAFSGIPLGYLEGDSVWGDDGR
                     HLGWWHRGWVLDHHGAAVLFTDLADGSGLQRPSKQVRPAKGLKGMAPRPATKHAKPVP
                     FLDGDRWSSRSGSHFFSSML"
     gene            complement(1132..1842)
                     /locus_tag="VF516_18195"
     CDS             complement(1132..1842)
                     /locus_tag="VF516_18195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109671.1"
                     /translation="MHHRQLLTVCGHFRCSAGFGISEHCTEVASAVLLVPSGGTTLAL
                     LDRETRALHVEADRGWVRLLRDSSTTRDDYLHQLSVTYGFESAYEAACSYTPGVGQAV
                     DLRGRWRSGLIAQDLLTLGCTAHDVEAMRCYSLAPFQDAAEALAWMYVVERPTSIHED
                     VRDELVSRFVDLARATTYLGAYEGAVSKRRAELGIALDQLCISDKVCKRVIEAVGAGF
                     HALSEWQRTSNPTLRSVG"
     gene            complement(2168..2314)
                     /locus_tag="VF516_18200"
     CDS             complement(2168..2314)
                     /locus_tag="VF516_18200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109672.1"
                     /translation="MSFRSPLSYFHNGFAKDLWAAVDFYDQRFHIGFTRQERSDLVAF
                     LRSL"
     gene            3104..4909
                     /locus_tag="VF516_18205"
     CDS             3104..4909
                     /locus_tag="VF516_18205"
                     /inference="COORDINATES: protein motif:HMM:NF045464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109673.1"
                     /translation="MPIQRKEAAEAGEPAPDVHAAGIGIAGNSRAGAPSAAVQRKPDA
                     PSAGSSGGSPGITPPTGGINKTGFIDNSDGANIRTGPAESGGQKIRDQPLPPATRVFV
                     SGTHPNASAWWYVTAYLGGTMVRGYAQDFRITTELPEPTAKLHQVIGGETAEQLASLE
                     YGSAVRDGHDLRYYENVLLYVNQQQHRAGISGSYQDPGVLGRGSNNVQLIAGHRIWLV
                     SPAYAKALEAVVPSGSLTGGTIAKVKRFVGHLEDILHSVTRSPQYFGEVAGEYAQAIR
                     DHMPEIVGIVAGFIMAEATSAFLAATPTGVGQIAATVIQLGLAAFGAAGAIQAGVEAL
                     KHAGQWLTLAWTAQGKDDRIADASKEFLKMLVSIAVTALSALGARANYGNAIKIASSM
                     PTGGLPAMAVAGGGQISGGTGAGTGVLINPGTGSFGTAGNAMMQADKDGGGGSKGDRA
                     EKQAEGSGTSKDDRATKQTGADQPQALASHWKEGDPLPNSDNALIDKAKFEKYSMDPS
                     NKGNGGKASAFKQIGYDIETEAGREVGASDVVTQLKGQLKSTAAIKGKETGFGQRFEV
                     RVQIKGPSGEGTLVTVWQLERREPRLITNWLEVHK"
     gene            4906..5139
                     /locus_tag="VF516_18210"
     CDS             4906..5139
                     /locus_tag="VF516_18210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109674.1"
                     /translation="MNFKASVEDSVELVVEVPSEFRNEMIPRGTRGTVVERYEHPREG
                     YSIDLAIPDSSLVGGFRYENVVLIPEQFVVIHQ"
     gene            complement(5394..5780)
                     /locus_tag="VF516_18215"
     CDS             complement(5394..5780)
                     /locus_tag="VF516_18215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109675.1"
                     /translation="MAVLALIHWNRSASTDEQLHVHAFYRAILEEARQMYLTAPTSDR
                     GRSAALLTNCFAPALTIFGEALDDHLVGMLAPLANTPVIVRDICQILMRNGVTDDTLN
                     SIGSRLGIDLAATRGLAERLAPGPPT"
     gene            complement(5955..7334)
                     /locus_tag="VF516_18220"
     CDS             complement(5955..7334)
                     /locus_tag="VF516_18220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109676.1"
                     /translation="MQDSPEQIEAWTRAEALLDPTCRTAVIGVLKPWAQAQLAARDGE
                     AAPPAAVLPPTLPSELRTALSEAAPTAPYSEWQFAAWSVRAEALVCELLAMAPNREAA
                     EALLRVHAPAPFWCALQQHFRLADSARCVAWVTFLAVPPTDLVASLSQETSLSFERLA
                     SEWARRRPFSLFEAGYDRSRPSWGPVATWERARMSLVLLLSPEAWMSALDELPLPTLM
                     WNAIQMLVLRDNPALIERLIATVPAAFDAAGSWTGSVAALLISDVVVEQLQATHGAAR
                     VAATSLELSMPGGSDRTDRAQQRLVALEKSELQAWATCMYASLLDRPGGDAVAFELLA
                     RLCRTHILGEWDHRAGAWSANETALAAMVRALVDRDVAVTTLEAWWRRREDDESRRRA
                     ATGASLETSTSVGMLSVPGLPYLVGAIAAVQSAMRIPAKRARMTPPRCGRGRRSSSWA
                     EIQALICCT"
     gene            complement(7334..9061)
                     /locus_tag="VF516_18225"
     CDS             complement(7334..9061)
                     /locus_tag="VF516_18225"
                     /inference="COORDINATES: protein motif:HMM:NF040700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPA1262 family N-terminal domain-containing
                     protein"
                     /protein_id="HEX8109677.1"
                     /translation="MSEVEAIACTAGFGETFADDYGSAVVHVVWERKKSQRTLLLAWV
                     ELLPREISSPDDEGEACTRVQDSYLYVRHVVTTADRALRWYRDCARGIAVRPANDGTL
                     PEPAPDVGTLAMSSTDDEPRWPNLVCTDSASLPFLANWYACPRVHHLIQPGFTLSALW
                     NDTQQANARRWLSEQLHFDVADHGVLWGSVHLVAPNPVFRSLSQGLEHSEDRKQERLL
                     LHVQARDGHRADGLLLRFLERRPTGICVSRHWTLTGPTLVLQFDHILSETAIEIHDPR
                     RGLLWFESPAHFLTSVGVQMSIASGTRVISDERGPLLEVPLVTDMPPTSVGAPAPIGN
                     ASAQVLLAEASTKHEQHEQRRAMQRWFSGQHEEAIAIVHELIRHARRQVVIVDPYFAR
                     TELRFAVAVSRVVVPVSVLTSHDGLIELEKNGEPQDEFVASVARFRATPNSHPLEVRV
                     MAGAGIHDRFLVVDDRVFLLGSSLNRFGDRGTMLVELRDPATVRGELLRELSASKPLP
                     QWLTDRRAAQRAQVSADTTTVVNSTSTADTMPAIELASSTDTTPAVESTVTAETMPAI
                     ESISNEGEE"
BASE COUNT         1716 a         3027 c         3083 g         1507 t
ORIGIN      
        1 gtcgcgcgag cggatccacc gcgcggtctc gtagccgtcc atctcgggca tctggacgtc
       61 gagcaagacg acggcgaagt cactttcgag cagcctgcgg agcgcctcgg tgcctgacgt
      121 cgccttgacg atgcgttgcg aaagcggctc gagcgccgct tcgatcgcga cgaggttggc
      181 cgggatgtcg tcgacgacga ggatgtcaca gcccatgggc atggggccgt cgtcctcggg
      241 gaggagttgc gcggatgcta ccgacgttag gcaagaccac aatgagagcc atcttgtcga
      301 agcgacgagg agttttcaac catcatcacg cgaacatcca agcgtgcgcg agcgcccgct
      361 gcgcgatggc gcacatttct cccgccgaga cgtcgcggcg caactccggc cgttcctcgc
      421 ccggcgggtc ggcgcggccg acgtcgacga cgtcttgcaa gacgtctggc tccgcgatgc
      481 ccggactgtt catcacaaca tcgacgagaa gaaatgcgac cccgagcgtg acgaccagcg
      541 gtcgccgtcg aggaacggca ctggcttggc gtgcttcgtc gcgggtcgag gcgccatgcc
      601 cttgagtccc ttcgccggtc gcacctgctt ggatggccgc tgcaggccgc tgccatcggc
      661 gagatccgtg aacaacaccg cggcgccgtg gtgatccaga acccagccgc gatgccacca
      721 tccgagatgg cggccgtcgt ccccccatac cgagtccccc tccagataac cgagcggaat
      781 gccgctgaat gcgtacacgt gacgctcatc gtcgcagtac gcaacagcct tgccagcagc
      841 atcgtagaac gtgaggtcca tcgcgttctg tacctaaagt gctagcggct ccgtcgatgg
      901 ttgcaaccgc gtaatgtcgc agacaaagtc acggggcgcg tgggcacagc aggtgggcgc
      961 gtgcgctcgc gctggcgcct gcgatgccgg cccggcgacg agctgaagcg gtgtccccga
     1021 aggaaacgcc cgacgtgacg accgtcgtag gtccacgggt tgtctccgtc gcggtagccg
     1081 aacgagacgc cgctccatgt ccacaagaaa gtcatgcagg gtcgaccgga gtcagcccac
     1141 gctccggagg gtcggattgc tcgtacgctg ccactcggac agcgcatgga acccggcgcc
     1201 gaccgcctcg atcactcgct tgcagacctt gtccgagatg cagagctgat cgagcgcgat
     1261 gccgagctca gcccgccgct tgctcacggc gccttcgtac gcgccgaggt aggtcgtcgc
     1321 gcgcgcgagg tcgacgaagc ggctcaccag ctcgtcgcga acatcctcat ggatcgatgt
     1381 cggacgctcc acgacgtaca tccacgccaa cgcctcagct gcatcctgaa atggggccaa
     1441 ggagtagcac ctcatcgcct cgacatcgtg cgctgtacat cccagcgtga gcagatcttg
     1501 cgcgatcagt cccgagcgcc agcgcccccg gaggtcgacg gcctggccaa cgcccggggt
     1561 atacgagcac gccgcctcgt acgccgactc gaagccatac gtcacgctga gctggtggag
     1621 gtagtcgtct cgcgtcgtgc tgctatcgcg cagcagccga acccagcccc gatcagcctc
     1681 cacgtggagc gcacgcgtct cacgatcgag gagtgccaac gtggttccgc cggacggcac
     1741 gagcaacacc gccgatgcaa cctctgtgca gtgttcggaa ataccgaagc ccgctgaaca
     1801 ccggaaatga ccgcacaccg tgaggagctg gcggtgatgc accgaagcac actgcgcgct
     1861 tgggctcggt caagatcgac acggccgggg ggcgcccgcg atgccgttgc gctgaggtgc
     1921 cgcgttcaag tgcgcagaaa ttggccagtt atctgacaaa ggatccggcg ttcagctcgg
     1981 accggcggca cgcgctcgcg ccgtcggtct cagcgcccag ctcgggcccg gccgcacccc
     2041 gcgcatcgac gccatcccat cgtccacact gaccggacgc ctcggtcccc cgcctggtga
     2101 ccgaaaaggc cgaaagccgg cagggggaag gccggctctc ggtgggggtg ggggaccgaa
     2161 gacttgtcta gagcgagcgg aggaaggcga ccaggtccga gcgctcctgc ctggtgaacc
     2221 cgatgtggaa gcgctggtcg tagaagtcga ccgccgccca caggtccttg gcgaagccgt
     2281 tgtggaagta cgaaaggggc gatcgaaaac tcaagtgttg tcgacgcact gcaacccttt
     2341 caggtgcttc cgcgcggccg aattccgctg gggtccgctc ggtgccgctg agtcccgccg
     2401 gttctggcaa agccctggca aacgagagcg ccgtgcggcg agcgatgatc ccgaccggcc
     2461 cgggcgtgtc gtggcgcggc ccggtggcgc gctcatggtc ggccctgcgg tgcactcgcc
     2521 gtccgccagg cggggcgcga tcgcggtcgc tttcggtgcg cgcggtcggt ctgcgtgctt
     2581 gggccggccg gcgcacttgg tcggctcggt gctctcgcgg tcggcgcggc tctcgcgtcc
     2641 atctggcgct gcacgctgct ggatcagcgc gccgcccggc gacgcgctcg cggtcggcct
     2701 agcacgctcg ggtcgccctg ggcttgccac ccaggggtag agcacatgaa gtgtccactt
     2761 accaccgttg gtcggtccgc ctcatcggtg aggcggcgac acctgggcga tcatcacatc
     2821 atgggtggaa ccaccgcatg gatcctcgcg ctctcgaggc gagaacgtct ggcgacgcgt
     2881 tcagccacca agttcggccg gacgactgta tagtcccggt cgccagccac gccgtgggtg
     2941 acagggagga ctagggagtg ccgagatgac agatcatgac caaagaagcg gtgccaggcg
     3001 accaggcggc gcaaccgtct cggctctcca cacaggcacc gcggctcagt cggcgcccgg
     3061 gccaggcaag cggacgctcg tcgagtcgct ggccccgccg ccgatgccca tccagcgcaa
     3121 ggaagctgcc gaggctggtg agcctgcgcc ggacgttcac gccgcaggga tcggcatcgc
     3181 gggcaactcg cgcgctggcg ctccgagcgc agccgtgcag cgcaagccgg acgccccgag
     3241 cgcgggatca tccggcggca gccccggcat cacgccgccg acgggcggca tcaacaagac
     3301 cggcttcatc gacaacagcg atggcgcgaa catccgtacc ggccctgccg agtccggtgg
     3361 ccagaagata cgcgaccagc ctctgccgcc agcgacgcgc gtgttcgtga gcggcacgca
     3421 cccgaacgca tccgcctggt ggtacgtgac ggcctacctc ggcggcacga tggtgcgcgg
     3481 ctacgcccag gacttccgca tcaccaccga gctgcccgaa ccgacggcca agctccacca
     3541 ggtcatcggc ggcgaaaccg ccgagcagct cgcctccctg gagtacggca gcgccgtccg
     3601 cgatggtcac gacctccgct actacgagaa cgtgctgctc tacgtcaatc agcagcagca
     3661 ccgcgccggc atctccggca gctatcagga cccgggcgtg ctcggcagag gcagcaacaa
     3721 cgtgcagctc atcgccggcc atcgcatctg gctggtcagc ccggcctacg ccaaggcact
     3781 cgaggccgtc gtgccatccg gctcgctgac cggtggcacc atcgccaagg tcaagcgctt
     3841 cgtcggccat ctcgaggata tcctgcacag cgtaacccga tcgccgcagt acttcggcga
     3901 ggtcgccggt gagtatgccc aagcgattcg cgaccacatg cccgagatcg tcggcatcgt
     3961 cgccggcttc atcatggccg aggcgacctc ggcgttcctg gccgcgaccc cgaccggcgt
     4021 cggccagatc gcggcaacgg tgatccagct tggcctggcg gcgttcgggg ccgccggtgc
     4081 catccaggcc ggagtcgagg cgctcaaaca tgccggccag tggctcacgc tcgcctggac
     4141 tgcccaaggc aaggacgacc ggatcgcgga cgcgagcaaa gagttcctca agatgctggt
     4201 cagcatcgcc gtcacagcgc tctcggcact cggcgcccgg gccaactacg gcaacgcgat
     4261 caagatcgcc agctcgatgc cgactggcgg cctgccagcg atggctgtcg cgggcggcgg
     4321 gcagatcagc ggcggcacgg gggcgggcac cggcgtcttg atcaaccccg gcaccggatc
     4381 ctttggtact gccggcaacg cgatgatgca ggccgacaag gacggtggag gcggatcgaa
     4441 aggtgatcga gccgaaaagc aggcagaggg ttcgggcaca tcgaaggatg accgagccac
     4501 gaagcagact ggcgcagacc aaccgcaagc tctcgcttct cattggaagg aaggcgaccc
     4561 acttccaaac tcggataatg ccctcatcga caaggcgaag ttcgagaaat attcgatgga
     4621 tccgagcaac aagggcaatg gcggcaaagc aagcgcattc aagcaaattg gctatgacat
     4681 agaaacagag gcaggacggg aggttggcgc gagcgacgtc gtgacacaac tcaagggcca
     4741 gctcaagagc acggcggcga tcaagggcaa agagactgga ttcggccagc gcttcgaggt
     4801 ccgagtccaa atcaaagggc cttccgggga aggcaccctg gtcaccgtgt ggcaacttga
     4861 gcgccgagag ccgcggctga tcacgaactg gttagaggtg cacaaatgaa cttcaaggcc
     4921 tcagtcgagg acagcgtgga gcttgtggtg gaggttccca gcgagtttcg caatgaaatg
     4981 attcccaggg gaacgcgagg taccgtcgtc gagcgttacg agcacccaag agaaggctat
     5041 tcaattgatt tggcgatccc cgatagctcc ttggttggag gatttcgcta cgagaacgtg
     5101 gtgctcattc cggagcagtt cgtggtgata catcagtagc cgtacatgtg cccccatgtc
     5161 tccggaatcg gttaggtcga aatttcctga acccgcaccc ggcgacgctt ggtcgcctcg
     5221 tcccaaccca cccaggtatc ccgcagcggc gctcggaagg tggcccgttg atggtctgcc
     5281 gacctcgtga cccgacaccg ctccgccacc ttgacctgac tcagcggcgg cggcaccaag
     5341 gagcctacgc ggcggcttga ccggatgatg ccggcaccgc cgatccgtcg cgttcacgtt
     5401 ggggggccag gtgcaaggcg ctctgcgagt ccgcgtgtgg ccgcgaggtc aatcccaagg
     5461 cggctgccga tgctgttcag ggtgtcatcg gtaacgccgt tgcgcataag gatttggcag
     5521 atgtcgcgga cgatcaccgg ggtgttggcg agcggagcga gcatgccgac caagtgatcg
     5581 tcgagtgcct caccgaagat ggttagagcg ggcgcgaaac aattggtgag caaagctgcg
     5641 gagcggcccc gatcggatgt cggagcggtc aggtacatct gccgtgcttc ttcgaggatc
     5701 gctcggtaga acgcatggac gtggagctgt tcatcggtcg atgcggaacg attccagtgg
     5761 atcagcgcca gcaccgccac gaagccgaga tcgtagctac cgtggagctt tcgcccccgt
     5821 aggctcgaac tcgtggtaag caagccttcg tcgctgcggc gcaagctttc gatgaagaac
     5881 tctccacgcc tcgtccggcc gacgttgcgc tgccagcaag tagccgagcc aatcgaagcc
     5941 cgattggact cgcgctacgt gcagcagatc aatgcctgga tctcggccca ggaagagctt
     6001 ctccgcccac gcccacagcg aggcggcgtc atccgcgcac gcttcgcggg gatccgcatg
     6061 gcgctctgca cggccgcgat cgcgccgaca aggtatggca gccctgggac ggataacatc
     6121 cctacggaag tggaagtctc gagagaggcc cccgttgctg cgcgtcgtcg ggattcatcg
     6181 tcctcacgcc gtcgccacca ggcttcgagc gtggtgactg caacgtcccg gtcgacgagc
     6241 gcgcggacca tggcagcgag tgcggtctcg ttggccgacc atgcgccggc acggtggtcc
     6301 cactccccga ggatgtgggt cctgcacagg cgcgcgagca gttcgaacgc gactgcatcg
     6361 ccacccgggc ggtcgagcag cgacgcatac atgcaggttg cccatgcttg gagctcgctt
     6421 ttctcgagcg cgacgaggcg ctgctgggcg cggtcggtgc gatcactccc ccctggcatc
     6481 gaaagctcaa ggctcgtcgc cgcaacgcgg gcagcgccgt gcgtcgcctg cagctgctcg
     6541 accacgacat cggagatgag tagcgcggca acactcccgg tccatgaacc agccgcatcg
     6601 aacgctgctg ggacagttgc aatcaaacgc tcgatcagcg cgggattgtc tcgcagcact
     6661 agcatttgga tggcgttcca catcagggtc ggaagtggca gttcatcgag ggcgctcatc
     6721 caagcttctg gtgacagaag cagcaccaac gacatccggg cgcgttccca ggtagctaca
     6781 ggaccccacg agggccggct gcgatcgtag ccggcctcaa agagtgagaa tggtcgacgc
     6841 cgcgcccatt ctgaggcgag ccgctcgaag gaaaggctgg tctcttgcga cagcgatgcc
     6901 accaggtcgg tcggtggaac cgcaagaaac gtgacccacg ctacgcagcg cgcgctgtcc
     6961 gcgagcctga agtgctgttg gagggcgcac caaaatggag ctggcgcatg aactcgaagc
     7021 agcgcttctg cggcttcgcg gttcggtgcc atcgcaagaa gctcgcagac gagcgcttcg
     7081 gcgcgaaccg accaagcggc gaactgccac tcactgtacg gtgcggtcgg cgcagcctcg
     7141 gacagcgctg ttcgcagctc acttggaagc gttggtggaa gcactgcggc tgggggtgcc
     7201 gcttcgccgt cccttgctgc aagctgggct tgggcccagg gcttgagcac tccgatcact
     7261 gccgtgcgac aggtcggatc gagaagggcc tccgcacgag tccaggcttc gatctgctcg
     7321 ggtgaatcct gcatcactcc tctccctcgt tggagatcga ctcgatggct ggcatggtct
     7381 ccgcggtcac ggtcgactcg acggctggcg tcgtatccgt gctcgaggcc aactcgatgg
     7441 ctggcatcgt gtccgccgtt gacgtcgagt tgacgacagt cgtcgtgtcc gcactcacct
     7501 gcgcccgctg cgcggcgcgg cgatcggtca gccactgggg caacggcttg ctggcggaga
     7561 gctcacgcag gagctcgcca cggacggtcg ctgggtcgcg caattcgacg agcatggtgc
     7621 cgcgatcgcc gaatcggttc agcgatgagc ccagcaggaa cacacggtca tcgacaacga
     7681 ggaagcgatc gtggatgccg gcaccggcca tgacgcggac ctcaagtggg tggctgttcg
     7741 gcgtcgcgcg aaagcgcgca acgctcgcga caaactcgtc ctgaggttct ccgttcttct
     7801 ctagctcgat cagcccatcg tgcgacgtca ataccgagac ggggacaacg acccgcgaaa
     7861 cagcgacagc gaacctgagc tcggtgcgag cgaagtacgg gtcgacgatc acgacctggc
     7921 gtctcgcgtg gcgaatgagc tcatggacga tggcgatcgc ctcctcgtgc tggccgctga
     7981 accagcgttg catcgcccga cgctgctcgt gctgttcgtg cttggtcgac gcttccgcga
     8041 gaagcacttg cgcgctggcg tttccgatcg gagcgggtgc cccgactgat gtcggcggca
     8101 tatcggtcac gagcggcacc tctagcagcg gtcctcgctc atcgctgatc acgcgcgtgc
     8161 cggatgcgat cgacatttgc acgcccacgc ttgtaagaaa gtgcgccggc gactcgaacc
     8221 agagcaggcc ccggcgggga tcatggatct cgatcgcggt ctcagagaga atgtggtcga
     8281 actggagcac gagcgtcggc cccgtcagcg tccagtgcct cgacacacag atgccggtcg
     8341 gccgtcgctc gaggaagcgc agaagcaggc cgtctgcgcg gtggccgtcc cgggcctgga
     8401 catgcagcag cagacgctcc tgcttgcggt cctccgaatg ctcgaggcct tggcttagac
     8461 ttcgaaacac cgggttgggc gcgacaaggt gcacggatcc ccagagtacg ccgtggtcgg
     8521 caacgtcgaa gtgcagctgc tcggagagcc atcgtcgtgc gttggcctgc tgggtgtcgt
     8581 tccacagcgc gctgagcgtg aacccgggct gtatgaggtg atggacgcgt ggacatgcgt
     8641 accagttggc aaggaacggc aagctcgccg agtccgtgca gacgaggttc ggccagcgcg
     8701 gctcatcgtc ggtcgacgac atcgccagag tacccacatc cggagctggc tcggggagag
     8761 tcccatcgtt ggcggggcgc accgcgatac ctctcgcgca gtcgcggtac cagcgcagtg
     8821 cgcgatccgc agtggtgacg acatggcgca cgtagaggta gctgtcctga acgcgcgtgc
     8881 aggcttcgcc ttcgtcgtcc ggagatgaga tctcgcgcgg cagcaactcg acccaggcca
     8941 gcagcagcgt acgctggctc tttttgcgct cccacaccac atggacgacc gcggaaccgt
     9001 agtcgtccgc gaacgtctct ccgaacccgg ccgtgcaggc aatcgcttcg acctctgaca
     9061 tgccaggcgg gcgatcgtag caggcaccag acttgccgct acggcagtcg tgacggcgcc
     9121 cgaaaatggc aagaaccggc cgcggtcggt tctggtctcg acagattgcc gagcagtgct
     9181 tacagcgagc tcaggaaggc gaccaggtcc gacttctcct gcttggtgaa tccgatgtgg
     9241 aaccgctcgt cgtagaagtt gacgaccgcc cacaggtcct tggcgaagcc gttgtggaag
     9301 tacggggcgc gcggggcgag gttgcgcagc atc
//
LOCUS       DASVOR010000943         2721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67712,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000943 DASVOR010000000
VERSION     DASVOR010000943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67712"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VF516_18230"
     CDS             complement(<1..443)
                     /locus_tag="VF516_18230"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8109678.1"
                     /translation="MTTTSALPKQYDPSEVEPRWRRFWLENGFFHADEASPTVPYSIT
                     LPPPNVTGSLHIGHALGFTLQDILIRWKRMQGFNAMWMPGIDHASIAVHVLLEKDLQR
                     RENKTRFDLGRDEFLRQAWAWKERSGVRISEQEKLMGYSLDWPRER"
     gene            935..1453
                     /locus_tag="VF516_18235"
     CDS             935..1453
                     /locus_tag="VF516_18235"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin domain-containing protein"
                     /protein_id="HEX8109679.1"
                     /translation="MLNDAEKKLILNSWRLVVPIKDTAADLFYRRLFELQPSYQPLFK
                     NDLPAQKQKLIAMLAFVVKSLDWPDSAWRDTVAEEDDLFLVVLALGRRHSELYSVPDA
                     AYAAVGEALLWTLDYGLGKEFTPPVRAAWTKVYGLVATAMKMGRLSVQPRAAGDAIVD
                     HVVRNRAEAKRT"
     gene            1453..>2721
                     /locus_tag="VF516_18240"
     CDS             1453..>2721
                     /locus_tag="VF516_18240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109680.1"
                     /translation="MLLSGTLADLDVAGIAAMTSLGRTSLRLELRRSNGDLIGSMVLK
                     AGRVVSATAGEVRGREALRVILRAASDTRFLLAHEPLDFVLSSALASVDELHQLARGT
                     TASPPAPRSEIRGDDDADTDDDEDAFGETTLALDPELDPGLDPGLEPGSRPPTAPPAP
                     ARIASARIAMMQGRLDEFDLVTLLQTIGVGRQLIEIEVRDRAGVPLGAMRVKGGKLVA
                     AHAGDVTGIDAVSALGRDAEGFEFAAYRVVTAPRQLSELASVAELGIRPPDRRGRVVM
                     EGSLSDFDLPTLLHTVGCSRQHCAVEIGDGRSLCGTIGLKSGVVVSAKAGALTGIPAL
                     QQLITARRGDWFRLIQLAGELPDEVPLGPVGQVLLHADAPAPTRPPTSPRPPTSPRLP
                     TSTGATAPTLDERPAAALAEGLLVMEGRLAD"
BASE COUNT          378 a         1002 c          951 g          390 t
ORIGIN      
        1 cgctcgcgcg gccagtcgag cgagtagccc atcagcttct cctgctcgct gatccggacc
       61 ccgctgcgct ccttccacgc ccaggcctgg cgcaggaact cgtcgcgccc gagatcgaac
      121 cgggtcttgt tctcgcggcg ctggaggtcc ttctccagga ggacgtgaac cgcgatcgag
      181 gcgtggtcga tgccgggcat ccacatcgcg ttgaacccct gcatccgctt ccagcggatc
      241 aagatgtcct gcagcgtgaa gccgagcgcg tggccgatgt gcagcgagcc ggtcacgttg
      301 ggcggcggca gcgtgatcga gtagggtacc gtcgggctgg cctcgtccgc gtggaagaag
      361 ccgttctcca gccagaaccg ccgccaccgc ggctcgactt cggagggatc gtactgcttg
      421 gggagcgccg acgtcgtcgt cacgtgggga cgctagcacg ggacggccgg cccagccccg
      481 ggcccaccgg aacaggtggg acaggccgcg ccgcatgcac cgggaccgtc gttttctacc
      541 gagtgccgct tgatctcggc cggccgggtc gcccgcgccg cgcatcgcga gggccgttac
      601 agccgccgcg atccctgcgg tcgctccgcg atccctgcgc ggccgcgcgg ttcgcgtgaa
      661 cacggttgcc tgtaccggat acgcgacgcc ccgcgatcgc gacgcagcct cgtaatgcgt
      721 cgcacggcgt tcgatcgcgg tgtcgatcgc acacgtcgtc gcaaattgcg cgcgcagacg
      781 ccccctcacc gccggcgttt ccttattctc ctctcacgcg ccacccccgt gtcggcgccg
      841 cctcgacgca agccgctgca aaccaccgca gctggtgcgc acgcaggaac ggatcgacgg
      901 accgccggct ctgaatcgcc acgaggaacg caacgtgctc aacgatgcag aaaagaagct
      961 gatcctgaac agctggcgcc tggtggtccc gatcaaggac accgcggcgg acctgttcta
     1021 ccgacgcctg ttcgagctcc agccgagcta ccagccgctg ttcaagaacg acctgcccgc
     1081 gcagaagcag aagctgatcg cgatgctcgc gttcgtcgtc aagtcgctcg actggcccga
     1141 ctcggcctgg cgcgacaccg tggccgagga agacgacctc ttcctcgtgg tgctcgcgct
     1201 cggccggcgc cactcggagc tctacagtgt ccccgacgcc gcgtacgccg ccgtcggcga
     1261 ggcgctgctg tggaccctcg actacggcct cggcaaggag ttcaccccgc cggtgcgcgc
     1321 ggcgtggacc aaggtctacg gcctggtggc gaccgccatg aagatggggc ggctcagcgt
     1381 ccagcccagg gcggcgggcg atgcgatcgt ggaccacgtc gtgaggaacc gcgccgaggc
     1441 caagcgcacg tgatgctgct ctcgggcacc ctggccgatc tcgacgtggc gggcatcgcg
     1501 gcgatgacca gcctcggccg caccagcttg cggctcgagc tccgccgatc gaacggcgac
     1561 ctgatcggca gcatggtgct caaggccggc cgggtggtct cggcgaccgc cggcgaggtg
     1621 cgcgggcgcg aggcgctgcg ggtgatcctg cgcgcggcga gcgatacccg gttcctgctc
     1681 gcgcacgagc cgctcgactt cgtgctgtcg agcgcgctgg cctcggtcga cgagctgcac
     1741 cagctcgcgc gcggtacgac cgcgtcgccg ccggcgcccc ggtccgagat ccgcggcgac
     1801 gacgacgcgg acaccgacga cgacgaggac gcgtttggcg agaccacgct cgcgctcgat
     1861 cccgagctcg atcccgggct cgatcccggg ctcgagcccg gctcgcggcc cccgaccgcc
     1921 ccgcccgcac cggcgcggat cgcatcggcg cggatcgcga tgatgcaagg gcggctcgac
     1981 gagttcgacc tggtcacgct gctccagacc atcggggtcg gccgtcagct gatcgagatc
     2041 gaggtgcgcg accgcgccgg ggtcccgctg ggggcgatgc gcgtcaaggg tggcaagctg
     2101 gtcgcggcgc acgccggcga tgtcaccggc atcgacgcgg tgtccgcgct gggccgcgac
     2161 gccgagggct tcgagttcgc ggcgtatcgc gtcgtcaccg cgccccggca gctcagcgag
     2221 ctcgccagcg tggccgagct cggcatccgg ccgcccgacc ggcgcggccg tgtggtgatg
     2281 gaaggctcgc tgtcggactt cgatctgccc acgctgctgc acacggtcgg gtgcagccgc
     2341 cagcactgcg cggtggagat cggcgacggg cgctcgctgt gcggaacgat cggcctcaag
     2401 tccggcgtcg tggtgtcggc gaaggccgga gcactcaccg ggatccccgc cctccagcag
     2461 ctgatcaccg cgcggcgcgg cgactggttc cggctgatcc agctcgccgg cgagctcccc
     2521 gacgaggtcc cgctcggccc ggtcggccag gtcctgctcc acgccgacgc gccggcgccg
     2581 acccgtccgc cgacgtcccc ccgtccgccg acgtcccccc gtctgccgac gtcgaccggt
     2641 gccaccgcgc cgacgctgga cgagcgcccc gcggccgcgc tcgccgaggg gctgctggtg
     2701 atggagggcc ggctggcgga c
//
LOCUS       DASVOR010000944         5992 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_477188,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000944 DASVOR010000000
VERSION     DASVOR010000944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477188"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..545
                     /locus_tag="VF516_18245"
     CDS             282..545
                     /locus_tag="VF516_18245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109681.1"
                     /translation="MRWTRDSIVSELATWMLSGTVIDAQFMTRHGPKGLVAAVRRVFG
                     RFEAALNVAALHNAKLYPEGPPTRGTSAGPRIAFPVATPREPK"
     gene            637..1062
                     /locus_tag="VF516_18250"
     CDS             637..1062
                     /locus_tag="VF516_18250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109682.1"
                     /translation="MIALGGWTVHSARQPGTWQHVLATREGEIGGMTSSRTVIRADSM
                     FVALPHPRALGRMVEVRYRDRAVVVPVLDVGPWNIDDAYWERDERPASERGRGAYRTP
                     VNRAGIDLSDPVFAALGLPDNDVVDWRFVHRGYVVLPRL"
     gene            1186..1995
                     /locus_tag="VF516_18255"
     CDS             1186..1995
                     /locus_tag="VF516_18255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109683.1"
                     /translation="MPDPVHTQRIAIAARLYALALIAVFAVVPMGLILYLDRFQAPSL
                     LFMDHSFHVIAIAVATIEGLFVSYVSWRCYQKSGEPFVRWLTLGFLGFTVVYAPHGFF
                     TPLAHDQPWLFILYGPVSRLTMATCLFIAILVHGKPTDPPEVRGAGWWAWLVAFLVID
                     VAVALLALSSIAGAPPLRLSIEGGALCLSALCAVVLVVRRIGAPRMRFYFIATLFFSQ
                     ASLAFVLGKVWNHQWWLAHAIFASGFFLLSYGVVRAFLTTGAFSTADGEQR"
     gene            complement(2020..2883)
                     /locus_tag="VF516_18260"
     CDS             complement(2020..2883)
                     /locus_tag="VF516_18260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109684.1"
                     /translation="MASTGLSIQIYDSFSITTPDRKESGIRSYSQACSARWRRECEVD
                     RMRERSAGRGVSSSGSFVERPGVEPGPGKRTLVEQVYGAPVQRKAASDPQPLPDGDLA
                     TGDASARDGQEVADGSSGSASSNVLAQQVTAQTTQQRLDALKDRVDALHRAHNDTGKL
                     RMVVTAAGMKDQVYAEWNKWRSGVMKISNDAFDALQQVQAEIEKLDPKKPGDQALIDQ
                     AKKIEAHARQVFQDAHYNNIPGQTEVTVIEQTEDVHFTVTSRDLGGADDLQSYLDVIH
                     SKYKAASTSSP"
     gene            complement(2944..4200)
                     /locus_tag="VF516_18265"
     CDS             complement(2944..4200)
                     /locus_tag="VF516_18265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109685.1"
                     /translation="MRLCPLLAVAAIIACDRPGKPKDQPGATSAGSSAPTKASLPEGA
                     SRGSAAPSAHASGFEDAPELREIERLTEQGDKEGARAAATALIASPSGSFEPPTREPF
                     LKGSALFLRSFVAASEADTLADLEQAAAFGHPEAPYHLASHLLIASTSRRDGERQLLR
                     PKIESLFLAGAQLADERCMDVLEDIYKRSGQRDDQNYWGMLRRMSEADEQRRKLLDTA
                     FRETFTAEDRASIDHAMRAKSLSGGAVPPSQGGLPGRSTLTSLFVDVWMRKQLAFVWR
                     AFFNANTPDATVTEVFDHYRQTVRAEPFGQLFLVLPSHASSDGQLSLTHEQLAAAILP
                     GDVMIVLADGRSHYATVWAIDRPGGTILVLDPFWEFWQPTHNSMITRMEHVDYAPRRQ
                     LFRLSWPEVRNMLVAAIVVRDAVASR"
     gene            complement(4208..5551)
                     /locus_tag="VF516_18270"
     CDS             complement(4208..5551)
                     /locus_tag="VF516_18270"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8109686.1"
                     /translation="MTYDIFLAYAGADIERARTLYGLLAPQLKVFLAAETLQPGDNWA
                     DEISKAQRESKMTVLLVSSAAESAYYLREEITSAIDISRARPQEHRTIPVYLDGIPRD
                     TNEILYGLRNLQAIDAPNAGGLPGVAQRLKALTTQLDDLPPLQPVAKAGTAASQNSVA
                     LELHDRLCRLTLTEFETFLLRSGVSRELIPSELAPTGLRAVSVVQLLQQEGSEALVRA
                     LSALRVLDETQPRTQSHRNALVLVGIVALALGGGAGYLLIERDSRTLPSLTASIAATQ
                     EIPLIRRPQDKDGEPVYAESSRCQVNFGHNQRGDVPIRVTSIAMEHESVPLTEEQRRS
                     FDYDIDVANLRGHGIVEERQYEFAITGPNARGRYLANKDRADVVHLDNILARPEAAES
                     VSIAGKADDAYFGFTAILNLHGASAFERVRFVATYDVAGRTNQHAATQWIYVYRK"
BASE COUNT          929 a         2014 c         1971 g         1078 t
ORIGIN      
        1 tggctcgaag acgggcccag cccgaacctg cgcgtgcgcg caaggtccga cccgcgcaac
       61 ccgccgcgcg tcagctggag ctgggcttcg ggccagcgcc gccgccccag gtcgagctcc
      121 cgcttgcgcg acccgcagca ccggacgccg cgccgcgcgc cggatccccg gccgagatcc
      181 tgggcgaggt cgaacccgcg cctgcacccg atgcggcggc cgtgccggcg acggcgacgc
      241 cgggacctcc gccgggcctt ccgggcggag gtcgcggtcg cgtgcgctgg acgcgcgaca
      301 gcatcgtcag cgagctcgcg acctggatgc tgagcggcac cgtgatcgat gcgcagttca
      361 tgacgcggca cggcccgaag ggcctcgtcg ccgccgtccg ccgcgtcttc gggcggttcg
      421 aggcagcatt gaacgtcgcc gccttgcaca acgcgaagct gtacccggag ggcccgccga
      481 cgcgggggac gagcgccggt cctcgaatcg ccttcccggt ggcaaccccg cgcgagccca
      541 agtgagcgcg cggcgatggg gcgcgctgca cggcgaggcc gacggtcccg gcacccggta
      601 cgccggcgag cggggatcgc ggcgctgggc gctgcggtga tcgcgctggg cgggtggacg
      661 gtgcacagcg cgcgtcagcc ggggacctgg cagcacgtgc tggcgacgcg cgaaggcgag
      721 atcggcggca tgacctcgag ccgcaccgtg atccgggccg actcgatgtt cgtggcgctg
      781 ccgcacccgc gcgcgctcgg ccgcatggtc gaggtgcgct accgcgatcg agccgtggtg
      841 gtcccggtcc tcgacgtcgg cccgtggaac atcgacgacg cctactggga gcgcgacgag
      901 cgcccggcct ccgagcgcgg ccgcggcgcg tatcgcaccc ccgtgaaccg ggcgggcatc
      961 gacctgtccg acccggtgtt cgccgcgctc ggcctccccg acaacgatgt cgtcgactgg
     1021 cggttcgtcc accgcggcta cgtcgtgctg ccgcggctgt agcccggtgt ctccaccgct
     1081 gatcgcgcgg atgagctccg aggaggcgct gctcgcgaag cacttcggcg ccgcgtacga
     1141 ggcgtatcgc gctcgcacgt ggcggctgat accgtacgtg tacggatgcc ggatcccgtc
     1201 cacacgcagc ggattgcgat cgcggcccgc ctgtatgcgc tggccttgat cgcggtgttt
     1261 gcggtcgtgc cgatgggact gatcctgtat ctggaccggt ttcaggcccc gtccctgctg
     1321 ttcatggacc acagctttca cgtgatcgcg atcgcggtcg cgacgatcga aggactgttc
     1381 gtcagttacg tatcatggcg ctgctaccag aagtccggcg agccgttcgt tcgctggctg
     1441 acgctgggct ttctcggctt cacggtggtc tacgcgcccc acggcttctt cacgcccctg
     1501 gcgcatgacc agccatggct gttcatcctc tatggcccgg tgtcccggct gacgatggcg
     1561 acctgcctgt tcatcgccat cctggtccat ggcaagccca ccgacccacc ggaggtgcga
     1621 ggcgccggct ggtgggcctg gctggttgcc ttcctcgtga tcgacgtggc cgtggcgttg
     1681 ctggcgttgt cctcgatcgc cggcgctcca ccgctccggc tgtccatcga gggcggcgcc
     1741 ctgtgcctgt cggcgctgtg cgcggtcgtc ctggtggtcc gccggatcgg cgcgccgcgg
     1801 atgcggttct atttcatcgc caccctgttc ttttcccagg cgtccctggc cttcgtcctc
     1861 ggcaaggtgt ggaaccatca atggtggctg gcgcatgcga tcttcgccag cggcttcttc
     1921 ctgctgagct atggcgtcgt ccgcgcgttc ctgaccaccg gcgcgttctc caccgccgac
     1981 ggcgaacagc gctgacctcg ggttccccgg accgtcgcgc tacgggctgg acgtggacgc
     2041 cgccttgtac ttgctgtgga tcacatcgag atagctctgc agatcgtccg cgccaccgag
     2101 gtcgcgggat gtcacggtga agtggacgtc ctcggtttgc tcgatcaccg tgacctcggt
     2161 ctgacccggg atattgttgt agtgcgcatc ctggaagacc tgccgggcat gcgcctcgat
     2221 cttcttggcc tgatcgatga gcgcttgatc gccgggcttc ttggggtcga gtttttcgat
     2281 ctcggcctgc acttgctgga gcgcgtcgaa cgcgtcattg gagatcttca tcacccccga
     2341 gcgccacttg ttccactccg cgtacacttg gtctttcatc ccggcggcgg tcaccaccat
     2401 gcggagcttg ccggtatcgt tgtgcgcacg gtgcagcgca tcgacgcgat ccttgagcgc
     2461 atcgagccgc tgctgcgtcg tctgtgcggt gacctgctgg gccagcacgt tgctcgaggc
     2521 agatccgctg ctcccgtcgg ccacctcctg cccatcccgc gcagaggcgt cgccagttgc
     2581 caggtcgccg tccggcagcg gctgcggatc cgatgccgct ttccgctgca ccggcgcccc
     2641 gtacacctgc tcgaccagcg tgcgttttcc gggacctggc tcgacgcctg gacgctccac
     2701 gaaagacccg ctcgacgaca caccccgacc agcacttcgt tcacgcatcc gatcgacctc
     2761 gcattcccgg cgccatcgag cgctgcacgc ctgggaatac gatcgaattc cggattcttt
     2821 ccgatcgggc gtggtgatgc tgaaagaatc ataaatttga atacttaggc ccgtgctggc
     2881 caccaggcgc acctgcgcgg gaggcggcgc agcccttgcg ggtaaggtcg gtagccaggg
     2941 cagctaccgg gacgccacgg catcgcggac gacgatcgcg gccaccagca tgttacgaac
     3001 ctctggccag gacagtcgga acagctgccg ccggggcgcg tagtccacat gctccatgcg
     3061 tgtgatcatc gagttgtgtg tcggctgcca gaactcccag aacgggtcca ggaccaggat
     3121 cgttccgccg ggccggtcaa ttgcccacac agtcgcgtaa tggctacggc catcggcgag
     3181 aacgatcatc acgtcgccgg gaaggatcgc cgcggcgagc tgctcatgcg tcagggacag
     3241 ctgcccatcg ctgctagcgt gtgacggaag cacgaggaat agctggccaa atggctcggc
     3301 ccggacggtc tgtcgataat gatcgaatac ctcggtgacc gtcgcgtcgg gcgtattggc
     3361 gttgaagaac gcgcgccaga caaacgccag ctgtttgcgc atccagacgt cgacaaacaa
     3421 gctcgtcagg gtcgaccggc cgggtaaccc accttgcgag ggcggtacag cgccaccgct
     3481 gagactcttc gcccgcatcg cgtgatcgat cgatgctcga tcctctgccg tgaacgtctc
     3541 acgaaacgca gtgtccagca gcttcctcct ctgctcgtcg gcctcggaca tgcgccgcaa
     3601 catgccccag tagttctggt cgtctctctg gccggagcgc ttgtaaatgt cttcgaggac
     3661 gtccatgcag cgctcgtcag ccagctgcgc gccggcgaga aacaaagact cgatcttggg
     3721 ccgcagcaat tgccgctcgc cgtcgcgcct cgacgtcgaa gcgatgagca gatgcgacgc
     3781 gaggtggtac ggcgcctccg ggtgtccaaa tgcagcagcc tgctccaggt ccgcgagcgt
     3841 gtccgcctcc gaggcagcga cgaacgagcg cagaaataac gcgctgccct tgaggaaggg
     3901 ttcgcgcgtg ggcggctcga acgacccgga aggacttgcg attagcgcgg tggctgcagc
     3961 gcgcgcaccc tccttgtcgc cctgttcggt caagcgctcg atctcacgca gctccggggc
     4021 atcctcgaag ccagaggcgt gggccgacgg cgccgcggaa cctcgggacg cgccctccgg
     4081 cagggacgcc ttcgtcggcg cgctggaacc cgcggaggtc gctcccggct ggtccttggg
     4141 cttgcctgga cgatcacaag cgatgattgc ggcgaccgcg agaagcggac atagacgcac
     4201 ggtccagcta cttccggtac acatatatcc actgcgtcgc tgcgtgttga ttggtgcgcc
     4261 cggcgacatc gtacgtggcc acgaaccgga cgcgttcgaa agcgctcgcg ccgtgcagat
     4321 tgaggattgc agtgaacccg aagtaagcat catctgcctt tcccgcgatc gagacactct
     4381 ccgccgcctc gggccgtgcc aggatgttat cgaggtgaac gacatcggct cggtccttgt
     4441 ttgcgagata gcgtcctctt gcgttcgggc cggtgatcgc gaactcgtac tggcgctcct
     4501 ccacgatccc gtgaccgcgc aggttcgcca cgtcgatgtc gtagtcgaat gagcggcgct
     4561 gctcctcggt gagcggcacg gattcatgct ccatcgcgat cgatgtcacc cggatcggta
     4621 cgtcgccgcg ctgattgtgt ccgaaattca cctggcaccg gctggactcc gcatagaccg
     4681 gctcaccgtc cttatcctgc ggccgcctta tcaacgggat ctcctgcgtt gccgcaatgc
     4741 tggccgtcaa gctcggcagc gtacgcgagt ctctctcgat caagagatag ccggctccac
     4801 cgccgagcgc gagtgcgacg atgccgacca ggaccagcgc gttccggtgc gactgcgtcc
     4861 gcggctgcgt ctcgtcgagc acccgcagcg cggagagcgc tcgcaccaga gcttcggatc
     4921 cctcctgctg cagcagttgc acgacgctca cggcacgcag cccggtcggt gccaactccg
     4981 aggggatgag ctcgcgcgac acacccgagc gaagcaagaa ggtctcgaac tccgtcaagg
     5041 tgagtctgca cagacgatca tggagctcca atgccaccga gttctgcgac gcggccgtcc
     5101 ctgcctttgc caccggctgc agaggcggca ggtcgtcgag ctgcgttgtc agagccttca
     5161 gccgttgcgc gacgcccggt aagccgcctg cgttgggagc gtcgatggct tgaagattgc
     5221 gcaggccgta gaggatctcg ttcgtatcgc gcggtatccc gtcgaggtac accgggatcg
     5281 ttcggtgctc ttgcgggcgt gcgcgactta tgtcgattgc actcgtgatc tcttcacgca
     5341 gatagtaggc agactctgcg gctgacgaga ccagcaacac ggtcatcttg gactcgcgct
     5401 gtgccttcga gatctcgtcg gcccagttat cgccaggctg cagcgtctcg gccgccaaga
     5461 ataccttgag ctgcggagcc aggagaccat acagcgtcct tgccctttcg atatcggcgc
     5521 cagcgtacgc caggaaaata tcatacgtca tgcctgcacc cgcaagccac atggtagggc
     5581 aagagatgct cgatggcgat ggcatgcgta aaccggtgat tgcatcgctt cgcccgagcg
     5641 cacgtatgac ccctcgagag caggataacc cggcaagacg caccgctcac ggtgcccggc
     5701 acggcaattt tgcagttgga ctgtgacctg caggctggtc tcgtcggcaa cgaggcgtgc
     5761 gctccgagct gcctcacgcc gcttgccacc gcgaggcgag cgggcggaag tgatagactg
     5821 ggacatcgcg gtgaatgatc cggctgagcc ggcgatcacg gcatcgggta atggcggagc
     5881 tgcgcgagct cgcggcggag ctcgccgcgg atgtcggggt gggcgatcga gatcagggcc
     5941 tcgccgcgct cgcgcagggt gaggccgtga aggttgcgcg cgccgtactc gg
//
LOCUS       DASVOR010000945         3223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_506096,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000945 DASVOR010000000
VERSION     DASVOR010000945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506096"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VF516_18275"
     CDS             <1..239
                     /locus_tag="VF516_18275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109687.1"
                     /translation="RISTSAQMTSGGTIYVDKFSCDDGYGITEELPVGLYDVTVTVTA
                     SPGTDLGASDPIPDKPITAPNGLTSLGHVKIIVH"
     gene            708..1184
                     /locus_tag="VF516_18280"
     CDS             708..1184
                     /locus_tag="VF516_18280"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8109688.1"
                     /translation="MCRTFASRARIDAPPRPALHGGPSPELDGDVDAWSPEHMLLSSL
                     GLCMLTTFEAFAARDGIELLAWRARVHGTVEQTPEGPNFTSIVLELDIDITGNVDQFE
                     DTLEDAKRYCLVQNALRVPVVVETAVRTPCDPLRAVDDDIPLRPRLAGDGRALHAM"
     gene            complement(1191..2573)
                     /locus_tag="VF516_18285"
     CDS             complement(1191..2573)
                     /locus_tag="VF516_18285"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8109689.1"
                     /translation="MDITTERPVVEVAATRPTISPAVADRHRDKVARIAAQLRTLPLG
                     RPVSLRKASVSHQVPKRHDLRRRDDKIDVSDLTEILEIDPVRRICVAESGVTFVDLVA
                     ATLPHGLVPIVVPELKTITIGGAVAGCSIESMSFVHGGFHDTCLEYEVITATGEVLIC
                     TPDNEHHLVFQMMHGAFGTLGILSRLTFKLIPAKPYVRLRYETCSTVAGYQAAIQRHF
                     EARDVDFMDGIIHSPTCYVLATGTFSDTAPYTSDYSWMKIYYQSTLTRTEDYLTTTDY
                     FFRYDRGVTNVRPRSWIGRLVLGKFLASSQWLRLGEKLRWALRAERPTITLDVFVPFS
                     KVPEFLTWYASTFQFYPLWCVPYRRVRDYEWLDESFYRTLPDPLFLDLAIYGMRQRGP
                     VNYHRVMEEKLRELGGIKTLIAHNYYGEAEFWSIWNKRNYDAVKAITDPDNRFRDLYT
                     KTCKTVMGRR"
     gene            complement(2650..3072)
                     /locus_tag="VF516_18290"
     CDS             complement(2650..3072)
                     /locus_tag="VF516_18290"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8109690.1"
                     /translation="MAIEIPTPEAVNQFFAREFPSGHAAGHHCIETGAGFAVARWTYD
                     ATQLRPGSLISGPTQFALADLAFWFATFTVVGLAPMAVTSELYISFLRPAKGGDLLAR
                     AELLRAGRSKISGTVRVWVDGAPDRPVSHLTGSYVHVQ"
BASE COUNT          515 a         1120 c         1097 g          491 t
ORIGIN      
        1 gccggatctc gacctcggcg cagatgacga gcggcggcac gatctacgtc gacaagttct
       61 cgtgcgacga cggctacggg atcaccgagg agctgccggt cgggctctac gacgtgacgg
      121 tgaccgtcac cgcctcgccc ggcacggacc tcggcgcatc ggatccgatc cccgacaagc
      181 cgatcacggc gcccaacggg ctgacgtcgc tcggccacgt caagatcatc gtccactgag
      241 cggcgtgagc gggcggccgg ccggtcgacg catgacggca cacgaaaagc ctgcacaccc
      301 acgacctccg actgcgtacg atgtttgacg tcgggatttc actgaaatcc cgcgcatgcc
      361 gcagctcgtc atggaggtcg ggccagtggc cggcctacga cgcatcgcag tgcggccgtg
      421 cgctcggagc ccgtccgaat ccagggcgca catcgcattc gctcgtcgat cccacgggga
      481 gctccgcccc cgccccgccg gcggagccgt cggggtcccc acccccgtct cgctgcgctc
      541 gatcgccgca gcgccgggcc tccgccgtgc gatgtcggcc acggccgccc gtgcgcgacc
      601 gtgcgggcgc ggcggcagcg cacatcgcgc gggtgctccc gcctggatca tttcgtgctc
      661 cggctcccta catgccggct ccgttccctc accgatacag cgcatcgttg tgtcgaacgt
      721 tcgcttcgcg tgcgcggatc gacgctccgc ccaggcccgc gctccatggc ggcccgtcgc
      781 cagagctcga cggcgacgtc gacgcgtggt cgcccgagca catgttgctg tcgtcgctcg
      841 ggctctgcat gctgaccacg ttcgaggcgt tcgcggcgcg cgacgggatc gagctgctcg
      901 cgtggcgcgc ccgggtgcac ggcaccgtcg agcagacccc cgaggggccg aacttcacct
      961 cgatcgtgct ggagctcgac atcgacatca ccggcaacgt cgaccagttc gaggacacgc
     1021 tcgaggacgc caagcgctac tgcctggtgc agaacgcgct gcgcgtgccg gtcgtggtcg
     1081 agaccgcggt ccgcacaccg tgcgatccgc tgcgcgcggt ggacgacgac atcccgctgc
     1141 gcccgcggct ggcaggcgac ggccgcgcgc tgcacgccat gtgacctcgg tcagcgccgg
     1201 cccatcacgg tcttgcaggt cttggtgtac aggtcgcgga agcggttgtc cggatcggtg
     1261 atcgccttga ccgcgtcgta gttgcgcttg ttccagatcg accagaactc ggcctcgccg
     1321 taatagttgt gcgcgatcag ggtcttgatc ccgccgagct cgcgcagctt ctcctccatc
     1381 acgcggtggt agttgaccgg cccgcgctgg cgcatgccgt agatcgcgag atcgaggaac
     1441 agtggatcgg gcagcgtgcg gtagaacgac tcgtcgagcc actcgtagtc gcggacccgg
     1501 cggtacggca cgcaccacag cgggtagaac tggaacgtgc tcgcgtacca ggtcaggaac
     1561 tcgggcacct tcgagaacgg gacgaacacg tcgagcgtga tcgtcgggcg ctccgcgcgc
     1621 agcgcccagc gcagcttctc gccgagccgc agccactgcg acgacgcgag gaacttgccg
     1681 agcaccaggc ggccgatcca cgaccgcggg cggacgttgg tgacgccgcg atcgtagcgg
     1741 aagaagtagt cggtggtggt gaggtaatcc tcggtccgcg tcagggtgct ctggtaatag
     1801 atcttcatcc agctgtaatc gctggtgtac ggcgcggtat cgctgaacgt gccggtcgcc
     1861 agcacgtagc aggtcggcga gtggatgatg ccatccatga agtcgacgtc gcgggcctcg
     1921 aagtggcgct ggatcgcggc ctggtagccc gcgaccgtcg agcaggtctc gtagcgcagc
     1981 ctcacgtacg gcttggccgg gatcagcttg aacgtcagcc gggacaggat gccgagcgtt
     2041 ccgaacgcgc cgtgcatcat ctggaacacc aggtggtgct cgttgtccgg cgtacagatc
     2101 aggacctccc cggtcgccgt gatcacctcg tactcgaggc aggtgtcgtg aaacccgccg
     2161 tggacgaacg acatcgactc gatcgagcag cccgccaccg ccccgccgat cgtgatcgtc
     2221 ttgagctcgg gcaccacgat cggcaccagg ccgtgcggca gcgtcgccgc caccaggtcg
     2281 acgaacgtca cgccggactc ggcgacgcag atccgccgca ccggatcgat ctcgaggatc
     2341 tcggtcaggt cgctgacgtc gatcttgtcg tcgcggcgcc gcagatcatg ccgcttgggg
     2401 acctggtgcg agaccgacgc cttgcgcagt gacaccgggc gaccgagcgg cagcgtgcgc
     2461 agctgcgcgg cgatccgcgc gaccttgtcg cgatgccggt cggcgaccgc cgggctgatc
     2521 gtgggccggg tcgccgcgac ttcgacgacc ggccgctcgg tggtgatgtc catatggaat
     2581 aggcgtagca atcgcgagcg ggctgtcatc cccgcgcttc gtgatttcgc cgcgagcccg
     2641 ccgcgtgcgt cactggacgt ggacgtacga gccggtcaga tgcgacaccg ggcgatcggg
     2701 cgcaccatcg acccacaccc gcacggtgcc gctgatcttc gaccggccgg cgcgcagcag
     2761 ctcggcgcgt gcgagcagat cgccaccctt tgccggccgc aggaacgaga tgtacagctc
     2821 cgaggtcacc gccatcggcg cgagcccgac cacggtgaac gtcgcgaacc agaacgcgag
     2881 gtcggccagc gcgaactggg tcggtcccga gatcaggctg ccaggccgca gctgggtcgc
     2941 gtcgtacgtc cagcgcgcga ccgcgaaccc ggcgccggtc tcgatgcagt gatggcccgc
     3001 ggcgtgcccc gacgggaact cgcgcgcgaa gaactgattg accgcttcgg gggtcgggat
     3061 ctcgatggcc acgcccgacc atatcgcagc cggcgcgcgc tcataagtaa aagcccaggc
     3121 cgaggccgcc gagcacgagc gccgtgtgcc catcggcgag gacccgggcg tgcacctgga
     3181 gcgcgaagtg ccggagatgg agcagctcca cacccgctcc gac
//
LOCUS       DASVOR010000946         4175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_417993,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000946 DASVOR010000000
VERSION     DASVOR010000946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4175
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417993"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..532)
                     /locus_tag="VF516_18295"
     CDS             complement(<1..532)
                     /locus_tag="VF516_18295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109691.1"
                     /translation="MLTVAVPSLPPPRMLSRWILIVWWGVAALCSAGHLVFFNLFDSD
                     DDLTWWGPIFVQFMICTAWALVTPLAFRIACDLGDDVRPARVARAIAWGLMLSAAMST
                     SRALLHGFEHRAGVVCSAGFTRIADYMTLDLPLFAAVLAAGLARRYLLQREAQQARAA
                     AIEVQLAESRLHALQMQ"
     gene            1089..1670
                     /locus_tag="VF516_18300"
     CDS             1089..1670
                     /locus_tag="VF516_18300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109692.1"
                     /translation="MKGISLWSIGACCLLAPWFAFADTSAACDPQTVEKADRGYTCVV
                     KTKNGPASWRVEAVSQGTRPVRVVKDLKSGLYVSDDLGKHSHESATKQKLCNSPEYSN
                     QRGNLASVAWRLPSGYPRRLNGKSGFPNRDSDFVVLEDDGIRQVISGLANKWFVSSSD
                     PGDGFSFGYDGEFGGIDTGCDGNGNVSLRCVGQ"
     gene            2406..3260
                     /locus_tag="VF516_18305"
     CDS             2406..3260
                     /locus_tag="VF516_18305"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8109693.1"
                     /translation="MALERMLRGAGLARAEGSVWCTPGAVGSGIHTPEFPEPTKAGSG
                     IDQVRISGTRHEPIIDGGKRAGARPGVRGDYQMFVLLTGLRRMDAATVRWKHLDLENA
                     RLHRPNPKGGKDRAFTIPLSTACVEILERRRRENMIAFPDGDGGWVFPTRAIKDKPCM
                     LCEALGLPGHAAGSVIHLVEGKQQKIERSTGKATRILPSPHRLRDTYTTACAEVGGLS
                     GYVIDVLTNHRPPRGSVTAGYIDLSTEHLAECQERVTSFLLARMKPGPTTGRESRRAG
                     RRRHLKAV"
     gene            3263..3442
                     /locus_tag="VF516_18310"
     CDS             3263..3442
                     /locus_tag="VF516_18310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109694.1"
                     /translation="MSSSDPSVDTDAFDRLWYVLNASFWYGLADVARASRLDHGVIDM
                     AEECSAAFFATGDSE"
     gene            3766..>4175
                     /locus_tag="VF516_18315"
     CDS             3766..>4175
                     /locus_tag="VF516_18315"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8109695.1"
                     /translation="MTAPVQRKTDADAAGERREAHAAPESRPSIKSLFGGQRKPMAAP
                     ASAEPASEPERHEVLDSAKAQRFGAQLGADFSGVTIHRGDGVAHDHGAAAIAFARDIH
                     FAPGMYETGGDALLSHELVHTVQQHGVPTTGPAPR"
BASE COUNT          738 a         1366 c         1428 g          643 t
ORIGIN      
        1 gctgcatctg cagcgcgtgc agcctggact cggcgagctg gacctcgatc gccgcggcgc
       61 gcgcctgctg ggcctcgcgc tgcagcaggt agcggcgcgc caggccggcc gccagcaccg
      121 cagcgaacag cgggagatcg agcgtcatgt agtcggcgat ccgggtgaac cctgcgctgc
      181 atacgacgcc ggcacggtgc tcgaagccgt ggagcagcgc gcgtgacgtg ctcatcgcgg
      241 cgctcagcat cagcccccac gcgatcgcgc gcgcgacccg ggccggccgg acgtcatcgc
      301 cgaggtcgca cgcgatccgg aaggccagcg gggtgaccag cgcccacgcc gtgcagatca
      361 tgaactgaac gaagatcggc ccccaccagg tgagatcgtc gtcgctgtcg aagaggttga
      421 agaagacgag gtgccccgcg ctgcacagcg cggccacgcc ccaccacacg atcaggatcc
      481 accgcgacag catgcggggc gggggcaggc tcggaaccgc gacggtcagc acacaggaca
      541 ccatactcga cgccgccggt ggcggtcgag gtgtgacctg aacggcagcc cggcgccacg
      601 agccgcggtc cccgcgtcac gaactgtgat cgaccgcgct gcgggcgttt acgatccagc
      661 gcgggccgcg ccgcgcgccg ggagccgcga tccgtgcccg cgatcctgcg accggtgcca
      721 ggctcgcggc gcaagcacct cgcatcgcgc agcctcgtac cgtgatgccg caacgcgctc
      781 gtatcgcccg catcgcggtc ccgatcttcc tcctgtcggc gcccgccggt gcgcgcgaga
      841 cgcccggcag ctcaccgcgg tccgcggggg cccggagcga tccaggtcga tggctcgctg
      901 gacgacccgg gctgggcggg cgtcgcgttc acggccgggt tcctccagaa gcggccggtg
      961 gatggcgcgg cgccttcgca gccgaccgag gtggcggtcg cgttcgatga cacgcccaga
     1021 attgctggca cagcgacggc gatgagtctc tcaacaagag gagaatctac atgtcagtac
     1081 atgctttcat gaagggtatc agtctttgga gcattggcgc gtgctgtctt cttgctccct
     1141 ggttcgcatt cgccgacacg tcggcagcct gcgatcctca aaccgttgag aaagcggatc
     1201 ggggttacac ctgcgttgtg aaaaccaaga acgggcccgc gtcgtggcgg gtcgaggccg
     1261 tatcgcaggg aaccaggccg gttcgggtgg tgaaggacct caagagcgga ctctacgtca
     1321 gtgacgatct gggcaagcac tcccacgaat cggcaacaaa gcagaagctg tgcaactccc
     1381 ccgagtattc gaaccagaga ggcaacctgg cgtccgtagc ctggcgactg ccgtccggat
     1441 atccgcgcag gctgaatggc aagagtggct ttccgaaccg cgacagcgat ttcgtggtcc
     1501 tggaggatga tggaattcgc caggtcattt ccggccttgc gaacaaatgg ttcgtctcct
     1561 cgtccgaccc gggtgatggt ttttccttcg gatacgacgg cgaattcggc ggaatcgaca
     1621 cggggtgcga cggcaacggc aacgtgtccc tgcgttgcgt tggccaataa tgccttccgt
     1681 cgcggctccg gtatgcggtg ccgatccgct cgcgcagtag aggacccgag ttgctcggag
     1741 acgttgtact cgtctcctcc ctggcggatc cgcgcggcgc cgcgactggt cgaccgtgcg
     1801 atcaccggcg agtcggcggt tgtctgcctc gcgaaggctg gccaatcgtg gatcgccgag
     1861 gtggtcgccg agctggcgag gtcaccttcg tgatcttggt gccgcgctcg gcccctaccg
     1921 gccggagcag ctcccgcgga cgctgtgggg cgcggtcgat gcgatggact tccggccgat
     1981 cgatcgaagc ggacaccggg tgaccatcga ggcaccggcc gccgtctggg aggccgcatt
     2041 cgagcacagc atgagtgagc agatcggggc gccatcgtaa ctcccccgcc atcgctcccc
     2101 ggtcaccgga tcgtgcaggc gcgggaagcg gcacactggg gtgcgtagct cagtccgtgg
     2161 aggatagggg gatcgaaccc ctgaccctcg gcttgcaaaa gaaacgcttt gccttgtgcc
     2221 aagcggttag gagctctacc agacggcgac taactcacga tcttcgacgt agagcatggg
     2281 ctttttcggt ggcgagttgt atccagccat ggcacgcttg gcgactagac ggcgactaat
     2341 tcgcctgccc acgccttgca gacgagactc accgagcgag ccctcgcttc agaacggatg
     2401 tagtggtggc gctcgaacga atgctccgag gagccggcct cgcgcgagct gaaggcagcg
     2461 tgtggtgcac gccaggagct gtgggttccg gaattcacac gccagaattt ccggaaccca
     2521 caaaggcggg ttccggaatc gatcaggtca gaatttccgg aacccgccat gagccgatca
     2581 tcgacggcgg aaagcgcgcc ggtgcccgcc cgggggtccg cggcgactat cagatgttcg
     2641 tgctgttgac cgggcttcga cgtatggatg ccgccaccgt acggtggaaa cacctcgacc
     2701 tcgaaaacgc caggctccac cgaccgaacc ccaagggcgg caaggaccga gcgttcacga
     2761 ttcccctcag cacggcgtgc gtcgagatcc tcgagcggcg acgtcgcgag aatatgatcg
     2821 cgttccccga tggcgatgga ggctgggtgt tccccacgcg cgccatcaag gacaagccgt
     2881 gcatgctgtg cgaagcgctc ggtctgcccg gacacgcggc tggttcggtg atccatctcg
     2941 tcgagggcaa gcagcagaag atcgagcgaa gcaccggtaa ggcgacccgg atcctgccgt
     3001 cgccgcaccg gctcagggat acgtacacaa ccgcatgcgc cgaggtcggg ggcctgagcg
     3061 gctatgtgat cgacgtcctg acaaaccacc gtccaccccg cggatccgtg acggctggtt
     3121 acatcgatct ctcgaccgaa catctcgcgg aatgccagga acgcgtaaca agcttcctgc
     3181 tcgctcgaat gaagccaggc cccacgaccg ggcgcgagtc gcgccgcgcg ggccggcggc
     3241 gtcatctcaa ggccgtctga agttgtcatc cagcgatccg tcggtcgaca ccgacgcgtt
     3301 cgatcggctc tggtacgtgc tcaacgccag cttctggtac ggtctggccg acgtcgcgcg
     3361 cgcgtcgcgg ctcgatcacg gcgtgatcga catggccgag gagtgctcgg cggcgttctt
     3421 cgcgaccggc gactcggagt agcggtccgc ccacttcggt aggcgccgca cctacgcccc
     3481 aagcccaacc gaaaaagacc gtagcgctgc ggcaagcact gcctcgtggc gcagcgctgc
     3541 aatatgcgtc ggtgatccag gacggaatgt cctggattcc gccgcgcgaa tattgaatgg
     3601 tcgaggctcg cggccatggg gtttggcggg ccgctcggaa agacagcgat gcgtgaggtt
     3661 ggaggcaagc ggtcgtcgac gacagcgggc cgtggcgagc aacccgcagg gccagggcca
     3721 gggccagggc caggcaagcg gacgctgacg gagacgcttc cggcggtgac cgcgccggtc
     3781 cagcgcaaga cggacgcgga cgcggccggg gaacgccgcg aggcacatgc cgcaccagag
     3841 tcgcggccct cgatcaagtc gctgttcggt ggccagcgca agccgatggc ggcgccggcc
     3901 agcgcggagc ctgcaagcga gcccgagcgc cacgaggtgc tcgatagcgc caaggcgcag
     3961 cgattcggcg cgcagctcgg cgctgacttc agcggcgtga cgatccatcg cggcgatggc
     4021 gtcgcacacg atcacggcgc ggcggcgatc gcgttcgctc gcgatatcca cttcgcgccg
     4081 ggcatgtacg agaccggtgg cgacgcgctg ctgagccacg agctggtgca cacggtgcag
     4141 caacacgggg tcccgacgac gggcccggcg ccacg
//
LOCUS       DASVOR010000947         7203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_126258,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000947 DASVOR010000000
VERSION     DASVOR010000947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7203
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126258"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VF516_18320"
     CDS             <1..220
                     /locus_tag="VF516_18320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109696.1"
                     /translation="AVAIAGGLPYRSYHWFLRFVMKLISRSAGHSTDTRRDHEYTDWA
                     RVQQLAADIARDLAPQQPARPSAPRAQA"
     gene            complement(323..1147)
                     /locus_tag="VF516_18325"
     CDS             complement(323..1147)
                     /locus_tag="VF516_18325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109697.1"
                     /translation="MDTGIGPESVMHGFDLLPRLPKERGEKEPGPFYARNAYPTPTGG
                     YYCFQIRNLAPESRRQIWAVVLNTASEVAAYGAFQRPQQDWLRNTLDSSQIKPTDLVL
                     VFAHNPIWDIFKQDERDALLDLLSSHRNVAGYFVGHVHTPQLRIVHPDKCYESGVKSS
                     EMHSECRKKDPRYTDDVEHHHFWEIVAPAVISYPQQARQVTVKMMGDVGYFEILSITP
                     RGTGDSAAAIDRALGGAALDYCHDHPDDCVDNHPRLPSRAVTYSRLFFQLPPLPPS"
     gene            complement(1164..2201)
                     /locus_tag="VF516_18330"
     CDS             complement(1164..2201)
                     /locus_tag="VF516_18330"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8109698.1"
                     /translation="MNQQEDAKHAASVELGVELGAPVAVASFLHFSDVQLREPGAKLG
                     GQSLSHQLDKIVKSFERNYGQELYSMFVYGALVRTANEELMLPPDPERPPPQFMIHTG
                     DAVDAGLTSEFEMFRNYSDFLQIPWYQVLGNHDVLAFGNLQLEDDATTDKKDDDRCQG
                     DRWERQAKNDECTCTRVSELIRELMLQDANPDNLGNSPDYSTVTSLLPIAVQKICIRH
                     HVDGDSFIMDPVHTAQPDAPAPMAQCDARNSTSTLASPWSASPRSASPRSTAQWSVNQ
                     RRTAQRPAHRGPRTSNSINTFIAAHCRSAGVPPQIDRGDGPCLQLPDCQPHVLPRGRP
                     GPLCSADPCDR"
     gene            2304..2597
                     /locus_tag="VF516_18335"
     CDS             2304..2597
                     /locus_tag="VF516_18335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109699.1"
                     /translation="MAAIRLDRVEAVDERVGGKRDVVAVIVGWLKFNWTTPSPTVVQG
                     CATARLATDPVAGAIGTVIQPRSYWTPMRDGPSAVSSTGETPNLPDSGSGSSS"
     gene            complement(2999..3487)
                     /locus_tag="VF516_18340"
     CDS             complement(2999..3487)
                     /locus_tag="VF516_18340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109700.1"
                     /translation="MHRTLILLVAATGACGKADRAPSPTPAPPPPLAHATQADLAREV
                     ADAERLGTWSDVSHRWQGQTVRWTVTHRRVLCRSADDCHVAAFPIQRPARQGWMPQLQ
                     FAPGQYDALTGLCGGQDPCEVTIEATLSKLEVSPELPASVQLSSVQIVPPPHPGTQTA
                     RR"
     gene            complement(3542..4807)
                     /locus_tag="VF516_18345"
     CDS             complement(3542..4807)
                     /locus_tag="VF516_18345"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 domain-containing protein"
                     /protein_id="HEX8109701.1"
                     /translation="MTKLIQSLAIAGLTLGAGALTASASSHREAPAIAEDQFVDNTDV
                     YAFISPSNANNLVIVADYVPLLLPASGPNFYKFSDNARYEVHIDNDGDAVPDIAYRWT
                     FRSQRKHGDTFLYNTGPVEAITSANLNVTQTYRLEKVNEHTGQTTTIMDNVPVAPWNV
                     GKRSFVNYEQVALQAVTTRNGTTSFAGPRDEPFFVDLHVFDLLGVGGAHTTDGVNVMS
                     LVLEVPITDIASKGARPAAGDTSPKSVVGIFASASRPRVRFLRDERDWDHYGNFVQVS
                     RLGWPLINEVIIPLQDKDKFNRSQPRNDVANFGQYILDPEVPKLLNLVLNANCAPTPA
                     GGRTDIVGLLSPSGTKAADLLRINITQGQTNASSHFPNGRALTDDVTDTLLTVACNNG
                     QHIGDGVDANDLPFTTTFPYLASPHSGNP"
     gene            complement(4821..5666)
                     /locus_tag="VF516_18350"
     CDS             complement(4821..5666)
                     /locus_tag="VF516_18350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109702.1"
                     /translation="MPTRPPTSVTKVSAGGFESPTDAVASPDGRTFYFAAWDMDKQPT
                     LFQVSSEPDSTPKPLVAGDPLEAPMGLVMSCDGSTLYVADMGGPAGAILAVATAGGQP
                     AVLGATGLTRPGGLAMGPDCKSLFATGRLDDSTPALFQVPIGGGAARVVYQGAPLVSP
                     TGLHVDGQGVAWVMDHIAQGLNGEGVLFAIPSDGSKATEVMSSLRMGTPGGVSLTAGG
                     GTAVMPTRDENGAAQLTSVHIATGAVTQFSTPDMAGPAGLRTARKAGVFVVVDAEAGT
                     IYRAE"
     gene            6068..>7203
                     /locus_tag="VF516_18355"
     CDS             6068..>7203
                     /locus_tag="VF516_18355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109703.1"
                     /translation="MAQRVESDITVQRFGFLSDDAGKRLRFPRAECHEIEGSAGPVGY
                     GARDCVLDDLEGRLDTLRWTADAASVGSAWLRDDAGRFEIAVGRVEMPRGLRLVRADR
                     GVEIVAPYVSLSEMKLTVRGPFGRTPGPDDPPAPPAPPTDPGLRQSKLRFLDSLSGRI
                     YFTVKVVLDLPVLGHRSLDQDLRIPIQEGSLDYRALEDSLNWLEGAFLDIKHDGDRLA
                     LVWKVPIVGSTRDLITWALDDEAAALAAFGRVPVRSLTDFRLAARASKPDDRKRKIVQ
                     SLSLNAIDIALSLLAPHSVELGGGVIMFGGDDQPGMVDLKVTGSLHDRGTGELKGAIG
                     SIDTTIKDVQLGPVKMTADRLHFDGIDELEVEFDGFRPVRATVVV"
BASE COUNT         1049 a         2400 c         2595 g         1159 t
ORIGIN      
        1 cgcggtcgcg atcgccggcg gcctgcccta tcgcagctac cactggttcc tgcgcttcgt
       61 gatgaagctg atcagccggt ccgccggcca cagcaccgac acccgccgcg accacgagta
      121 caccgactgg gcccgggtcc agcagctcgc cgccgacatc gcccgcgacc tcgcgcccca
      181 gcagcccgcg cggccgtccg caccgcgcgc ccaggcctag cgaggactgg cgatcgccac
      241 gaccccgccg cggagccagc aatccccatc gcatcgcggc atggggctac caccgcaaag
      301 cgcgcatcgc ggcgctacac gctcaagacg gcggcagcgg tggcagctgg aaaaacagcc
      361 gcgaataggt cacggcgcgc gacggcaagc gagggtggtt gtcgacgcag tcgtccgggt
      421 ggtcgtggca gtagtcgagc gcggcgccgc ccagcgcgcg atcgatcgcg gccgcgctgt
      481 cgcccgtgcc gcgcggcgtg atcgacagga tctcgaagta accgacgtcg cccatcatct
      541 tgaccgtgac ctgccgggct tgttgcggat acgagatgac cgcgggtgca acgatctccc
      601 agaagtgatg gtgctcgaca tcatcggtgt acctgggatc tttcttgcgg cactctgaat
      661 gcatctcgga agacttgaca ccgctctcgt agcacttgtc gggatgcacg atgcgcagct
      721 gcggcgtgtg aacgtgcccc acgaagtagc cggccacgtt gcggtggctc gacagcaggt
      781 cgagcagcgc atcgcgctcg tcttgcttga agatgtccca gatcggatta tgggcgaaca
      841 ccagcacgag atccgtcggc ttgatctggc tgctgtccag cgtattcctg agccagtcct
      901 gctggggtcg ttgaaatgcg ccgtaggcgg cgacctccga ggcggtattg agcacgaccg
      961 cccagatctg ccggcgagac tccggtgcga ggttgcggat ctggaagcag tagtatcccc
     1021 ccgtcggggt gggataggcg ttccgggcgt agaacggccc aggctccttc tcaccgcgct
     1081 ccttcggcag ccggggtagc aggtcgaaac cgtgcatcac tgactcgggg ccaatgccgg
     1141 tgtccaccgc gcaagggtgc gagtcatctg tcacagggat ctgccgagca tagtggtccg
     1201 gggcgtcccc gcggaagcac gtgcggctgg caatcgggca gttgcaggca aggaccatcg
     1261 ccccggtcga tctggggcgg gactccggcc gacctgcagt gagccgcgat gaacgtgttg
     1321 atcgagttcg acgttcgggg cccgcggtga gcgggccgct gggccgtgcg cctctgattg
     1381 accgaccact gggccgtgga cctcggggac gcggacctcg gggacgcgga ccacggggat
     1441 gcgagcgtgg aggtggagtt gcgggcatcg cattgcgcca tgggggccgg agcatcgggt
     1501 tgcgcggtat ggaccgggtc catgatgaag ctgtcgccgt cgacgtggtg ccggatgcag
     1561 atcttctgaa ctgcaatggg caagaggctg gtcaccgtcg agtagtcagg ggagttgccg
     1621 aggttgtccg ggttggcgtc ctgcagcatc agctcgcgga tcagctcgct gacccgcgtg
     1681 cacgtgcact cgtcgttctt ggcctggcgc tcccagcggt cgccctggca gcggtcgtcg
     1741 tccttcttgt ccgtggtggc atcgtcctcg agctgcaggt tcccgaacgc caggacatcg
     1801 tggttgccga gcacctggta ccaggggatc tgcaggaagt cggagtagtt gcggaacatc
     1861 tcgaactcgg acgtcaggcc ggcatcgacc gcgtcgccgg tatggatcat gaactggggt
     1921 ggcggccgct cgggatcggg cggcagcatg agctcctcgt tggcggtgcg caccagcgcg
     1981 ccgtacacga acatcgagta cagctcctga ccgtagttgc gctcgaacga tttgacgatc
     2041 ttgtcgagct ggtggctgag cgactggcca ccgagcttgg cgccgggctc gcgtagctgg
     2101 acgtcactga agtgcaagaa cgaagcgacc gccaccggcg caccgagctc gacgccgagt
     2161 tcgaccgacg cggcgtgttt cgcgtcctct tgctggttca ctgcccccga ggtgagccag
     2221 gatgtcctcg gatctgcctc gtagccgagc cagccctgga actgcgcgct ggcatcgcac
     2281 gggatcgcct ggaggtcgct gtgatggccg ccatccgcct ggatcgtgtc gaagcagttg
     2341 acgaacgggt gggcgggaaa cgtgatgtag tggccgtgat cgtggggtgg ttgaagttca
     2401 actggaccac tccctccccc acggtcgtcc agggctgcgc gaccgcgcgg ctggccaccg
     2461 acccggtcgc gggagcgatc ggcacggtca tccagccgcg atcgtactgg actcccatgc
     2521 gcgacgggcc gagcgcggtg tccagcaccg gtgagacgcc gaacctgccc gacagcggta
     2581 gcggatcatc gtcgtagtac gggcagccgt ccttccagtc gtcgtcagca gcagcgagca
     2641 ccggcgcgac tgctgcggcc agcgccacga gatacacgat ccgtgctcga tggaggcgcg
     2701 actgccatga ggccccctgc tggacatcgc tgacgcgctg gccccacact ccttgcaagg
     2761 tacatctccc gcgcgctccg atccacggag cctggcgcgg catgaccggg gcacttcgcc
     2821 tgtcctcgtt ctccaccgcg atcgagtgcg agttttctga tgctgttgga atactctgct
     2881 cgatgatggc cgctgggttc cggcgaatcc gccggggacg ccatcgcaaa acgtcacagc
     2941 ccttgtcgcg gaggccacgc gtcctccgcg acaagggctg cttcgctgcc gggtgccgtc
     3001 agcggcgggc tgtctgcgtg ccggggtgtg ggggaggtac gatctgcacg ctcgacagct
     3061 gcacgctggc cggcagctcc ggcgagacct cgagcttgct cagcgtggcc tcgatggtga
     3121 cctcgcacgg atcctggccg ccgcacaggc cggtcagcgc gtcgtactgg ccgggtgcga
     3181 actggagctg gggcatccag ccctgcctgg ccgggcgctg gatcgggaac gcggcgacgt
     3241 ggcagtcgtc ggccgaccgg cacagcacgc gccggtgcgt caccgtccag cgcacggtct
     3301 ggccctgcca gcggtggctg acgtcgctcc acgtgccgag ccgctcggcg tcggcgacct
     3361 cgcgggcgag gtcggcctgg gtggcgtggg cgagcggcgg cgggggggcc ggcgtcggcg
     3421 acggcgcgcg atcggccttg ccgcacgcac cggtggcggc gacgagcagg atcagggtgc
     3481 ggtgcatggc gggctcctcg gtacgtgcgg gtcgggtcgg gggtcggtca gcgcgctcgg
     3541 ctcacgggtt accggagtgc ggcgacgcca ggtacgggaa cgtcgtcgtg aacggcaggt
     3601 cgttggcgtc gacgccgtcg ccgatgtgct ggccgttgtt gcacgcgacg gtgagcagcg
     3661 tgtcggtcac gtcgtcggtc agcgcgcgac cgttggggaa gtgcgagctg gcgttggtct
     3721 ggccctgggt gatgttgatc cgcagcaggt cggccgcctt ggtgccgctg ggcgacagca
     3781 gcccgacgat gtcggtgcgg ccgcccgccg gggtcggcgc gcagttggcg ttgagcacga
     3841 ggttgagcag cttgggcacc tcggggtcga ggatgtactg gccgaagttg gcgacgtcgt
     3901 tcctgggctg gctgcggttg aacttgtcct tgtcctggag cgggatgatc acctcgttga
     3961 tcagcggcca gccgagccgt gacacctgga cgaagttgcc gtagtggtcc cagtcgcgct
     4021 cgtcgcgcag gaaccggacg cgcggccggc tcgccgaggc gaagatcccg acgaccgact
     4081 tcgggctggt gtcgccggcc gccgggcggg cgcccttgct ggcgatgtcg gtgatcggca
     4141 cctcgagcac gagcgacatc acgttgacgc cgtcggtggt gtgggcgccg ccgaccccga
     4201 gcaggtcgaa cacgtggagg tcgacgaaga acggctcgtc gcgcgggccg gcgaacgagg
     4261 tcgtgccgtt cctggtggtc accgcctgca acgcgacctg ctcgtagttg acgaacgacc
     4321 gcttgccgac gttccacggc gcgaccggga cgttgtccat gatcgtcgtg gtctgcccgg
     4381 tgtgctcgtt gaccttctcg agccggtagg tctgggtgac gttgaggttg gcgctggtga
     4441 tcgcctcgac cggcccggtg ttgtagagga acgtgtcgcc gtgcttgcgc tgcgagcgga
     4501 acgtccagcg gtaggcgatg tcgggcaccg cgtcgccgtc gttgtcgatg tggacctcgt
     4561 agcgcgcgtt gtcggagaac ttgtagaagt tcggacccga cgccggcaac agcagcggga
     4621 cgtagtccgc gacgatcacc aggttgttgg cgttgctcgg gctgatgaac gcgtagacgt
     4681 cggtgttgtc gacgaactgg tcctcggcga tcgcgggggc ttcgcggtgc gacgaggcgg
     4741 aggcggtgag cgcgccggcc ccgagggtga ggccggcgat ggcgagtgac tggatgagct
     4801 tggtcatgac ggtctcctgt tcattctgcg cggtagatgg tgccggcttc ggcatcgacg
     4861 acgacgaaca cgcctgcctt gcgcgcggtg cgcaggccgg ccgggcccgc catgtcgggg
     4921 gtggagaact gggtgacggc gccggtggcg atgtggacgg aggtcaactg cgccgcgccg
     4981 ttctcgtcgc gggtcggcat gaccgcggtg ccgccgcccg cggtgagcga cacgccgccc
     5041 ggcgtgccca tccgcaggct cgacatcacc tcggtcgcct tgctgccgtc ggacgggatc
     5101 gcgaacagca cgccctcacc gttgaggccc tgggcgatgt ggtccatcac ccacgcgacg
     5161 ccctggccgt cgacgtgcag gccggtcggc gacaccagcg gagcgccctg gtacacgacg
     5221 cgggcggcgc cgccgccgat cggcacctgg aacagcgccg gcgtgctgtc gtcgagccgg
     5281 ccggtcgcga acagcgactt gcagtccggc cccatcgcca ggccgccggg ccgggtcagc
     5341 ccggtggcgc cgagcacggc cggctgtccg cccgcggtcg cgaccgcgag gatcgccccg
     5401 gcaggaccgc ccatgtcggc gacgtacagc gtgctgccgt cgcacgacat caccagcccc
     5461 atcggcgcct cgagcggatc gccggcgacg agcggcttcg gcgtgctgtc cggctccgac
     5521 gagacctgga acagcgtggg ctgcttgtcc atgtcccacg cggcgaagta gaaggtgcgg
     5581 ccgtccgggc tcgcgacggc gtcggtcggc gattcgaagc cgccggccga gaccttggtg
     5641 acggaggtcg gtggacgagt aggcaaacca tcatcagtgg agtggcatcc gatcaacgcc
     5701 gcaggcgtcg aagcgagcac caccaagagt cgtgtggcca tgtggccctc cctggctgtg
     5761 tgccgacata cgcgcgacct gcgagaacgg atcagcttgt cgcgcgagcg tggaagtcat
     5821 cgggagcgca tgcgaaccgc tcgccgatcg cgatcccctc gccgctggag gtcgccgcca
     5881 ccggctgcgg cgatgtcaag tgcgggctgg cgtacttcgg gacgcgcggc gatccgtgcg
     5941 cccgcgccca gcccgcgccc agcggcccgc ctgcgggaag gggaacctcc ggtgagacgc
     6001 ggatctcccg cgggaggagc cgcctgcccg ccaccccggg ggtcgaccaa gtacactcgt
     6061 cagccggatg gcccagcgag tcgagagcga tatcaccgtc cagcgcttcg ggttcctgag
     6121 cgacgacgcc ggcaagcggc tgaggttccc gcgcgccgag tgccacgaga tcgagggctc
     6181 cgccggcccg gtcggctacg gcgcccgcga ctgcgtgctc gacgacctcg agggccggct
     6241 cgacacgctg cgctggaccg cggacgccgc gtcggtcggc tcggcgtggc tgcgcgacga
     6301 cgccggccgg ttcgagatcg cggtcgggcg cgtcgagatg ccacgcggcc tgcgcctggt
     6361 tcgcgccgac cgcggcgtcg agatcgtcgc gccctacgtc tcgctgtcgg agatgaagct
     6421 cacggtgcgc ggcccgttcg gccggacacc ggggccggac gacccacccg cgccgcccgc
     6481 cccgcccacc gacccggggc tgcgccagtc caagctgcgc ttcctcgaca gcctgtcggg
     6541 ccggatctac ttcaccgtca aggtcgtgct cgacctgccc gtgctcggcc accgctcgct
     6601 cgatcaggac ctccggatcc cgatccagga gggcagcctc gactatcgcg cgctcgagga
     6661 cagcctgaac tggctcgagg gcgcgttcct cgacatcaag cacgacggcg accggctcgc
     6721 cctggtctgg aaggtcccga tcgtcggctc gacccgcgat ctgatcacct gggcgctcga
     6781 cgacgaggcc gccgcgctcg ccgcgttcgg ccgcgtcccg gtccgctcgc tgaccgactt
     6841 ccggctggcc gcgcgcgcca gcaagcccga cgaccgcaag cgcaagatcg tccagtcgct
     6901 gtcgctcaac gcgatcgaca tcgcgctgtc gctgctcgct ccgcactcgg tcgagctcgg
     6961 cggcggcgtg atcatgttcg gcggcgatga ccagcccggt atggtcgacc tcaaggtcac
     7021 cggctcgctc cacgaccgcg gcaccggcga gctcaagggc gcgatcggca gcatcgacac
     7081 cacgatcaag gacgtccagc tcggcccggt caagatgacc gccgaccgcc tgcacttcga
     7141 cggcatcgac gagctcgagg tcgagttcga cgggttccgc ccggtccgcg ccacggtcgt
     7201 cgt
//
LOCUS       DASVOR010000948        11678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_185044,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000948 DASVOR010000000
VERSION     DASVOR010000948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11678
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185044"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="VF516_18360"
     CDS             <1..272
                     /locus_tag="VF516_18360"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8109704.1"
                     /translation="GLVNRVVPRADLVAETHKLARAASRGSLTSKALGKRTFYQQIEL
                     DVEAAYAEAIEVMAQSAVTPDGRENMRAFIEKRHAVYSSTRPPTR"
     gene            complement(430..1674)
                     /gene="arsJ"
                     /locus_tag="VF516_18365"
     CDS             complement(430..1674)
                     /gene="arsJ"
                     /locus_tag="VF516_18365"
                     /inference="COORDINATES: protein motif:HMM:NF033734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="organoarsenical effux MFS transporter ArsJ"
                     /protein_id="HEX8109705.1"
                     /translation="MGSLRNYVLVTAAYWAFTLTDGALRMLVLLHFYRLGYTPVALAF
                     LFVFYEFFGVVTNLVGGWIAVRLGLRATLLGGLALQVAALALLALLEPTWGRSASVVY
                     VMTAQALSGIAKDLTKMSAKSAIKVLVPVGAESSLFKWVAVLTGSKNALKGIGFFLGG
                     FLLGALGFRESLFAMAGGIAITLIGSAASLPREIGKAKSKAKFSHIFSNNHAINVLSA
                     ARFFLFGARDIWFVVGAPVFLATRFAWGFMEVGGFLAAWVIGYGVIQSITPLLLRRFT
                     KGRAPQGRSATVLAFVLAAVIIGVIVGVEHDYQPELLVVAGLGLFGVAFAINSSVHSY
                     LILAYSDGDKVAMNVGFYYMANAGGRLAGTVLSGALYQRAGLIGCLWASAAFAATAGL
                     LAVLLPQVPEVAIDADADVGGE"
     gene            complement(1683..2693)
                     /locus_tag="VF516_18370"
     CDS             complement(1683..2693)
                     /locus_tag="VF516_18370"
                     /inference="COORDINATES: protein motif:HMM:NF033735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsJ-associated glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEX8109706.1"
                     /translation="MSIRIGINGFGRMGRLALRAAWSWPDVEFVHINEVQGGPEVAAH
                     LLSFDSVHGRWPNDVKTTRDGIIIDGKQLTFSEHGTPGAVPWTKYGVDLVLECSGKFR
                     TPALLAPYFDGGVKKVIVAAPVKEGALNIVVGINDHLYDPDKHHLVTAASCTTNCLAP
                     VVKVIHEGIGIRHGVITTIHDITNTQVVIDAPHKDLRRARASSMSLIPTSTGSATAIT
                     LIYPELKGKLNGLAVRVPLLNASLTDCVFETARPTTIEEVNHLLRVAAEGPLKSIMAY
                     EERELVSVDFKGDPHSAIIDAASTMVVDGTSVKILAWYDNEIGYVHRMMELARKVGKH
                     LH"
     gene            complement(2863..3228)
                     /locus_tag="VF516_18375"
     CDS             complement(2863..3228)
                     /locus_tag="VF516_18375"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8109707.1"
                     /translation="MLASASELDVRPLSLAFRALGDETRLRIVALLTHGELCVCHLEK
                     ALDLSQPNVSRQLGILRMAGLVNARREATWVYYSLSKQHHDLVESQLAALTKAFGAER
                     ALRADHARLRKSCGPDACK"
     gene            complement(4136..5251)
                     /gene="arsB"
                     /locus_tag="VF516_18380"
     CDS             complement(4136..5251)
                     /gene="arsB"
                     /locus_tag="VF516_18380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEX8109708.1"
                     /translation="MSRTGIASKLSFLDRYLTLWIFAAMALGIAIGFVAPGVTSALNR
                     LSIGTTSIPIAVGLILMMYPPLAKVRYEELGKVFRNRRVLLLSLVQNWIIGPLLMFGL
                     AVVFLRDHPEYMLGLILIGLARCIAMVIVWNDLAKGDTEYCAGLVAFNSIFQVLFFSV
                     YAYFFATLLPRWLGLQSAIVDITIREIAKSVAIYLGIPFAAGFITRRVLVGARGRDWY
                     EKRFVPRIGPITLVSLLFTIVVMFSLKGEAIVQLPLDVLRIAVPLLIYFVVMFTVSFV
                     MSKRVGANYRQSATLSFTAASNNFELAIAVAIASFGIHSGAAFAAVIGPLVEVPALIG
                     LVNVALWAQRRFYPSAAIEAVSPSRANDSAPTVASNA"
     gene            complement(5325..5654)
                     /locus_tag="VF516_18385"
     CDS             complement(5325..5654)
                     /locus_tag="VF516_18385"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8109709.1"
                     /translation="MRLFRALGDETRLRIVALLSHGELCVCHIEAALDLNQSTASRHL
                     GILRTAGVVDCRREGTWVYYRLTDQEHDTVAKALDVLTKAFGAERALRANHAKLRRSC
                     GPDACAS"
     gene            5719..5877
                     /locus_tag="VF516_18390"
     CDS             5719..5877
                     /locus_tag="VF516_18390"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109710.1"
                     /translation="MYTSLLFLCVANSARSQMAEGLARALFGGLVQVQSAGSQPTRVD
                     PNAIAAPA"
     gene            5948..6757
                     /locus_tag="VF516_18395"
     CDS             5948..6757
                     /locus_tag="VF516_18395"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEX8109711.1"
                     /translation="MKADDVRTVVREHYGNIARGAGTSCAPGCCSSAGTAAASLGYTA
                     DQTEAVPDGADLGLGCGNPTAIASLRDGETVLDLGAGGGFDCFIAAHQVGPRGRVIGV
                     DMTADMVARARANARKVNATNVEFRLGEIEHLPVADASVDVILSNCVINLSPDKPAVF
                     REAFRVLRAGGRLAISDVVATGPIPVELQNQAAALAGCVAGAAPLDDVRAMLAAAGFV
                     DVEVKISPRSAEIVGSWLPGIERFVASATIEARKPGGDAGASSCCGPECCA"
     gene            6754..7314
                     /locus_tag="VF516_18400"
     CDS             6754..7314
                     /locus_tag="VF516_18400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8109712.1"
                     /translation="MNASPAPWQDVAAQLRPFLARRVAAADVDDVLQNVWLRVQRGLP
                     GLRDEERFTSWLFQIARSAVAEHQRARARHPLPDAPAESDLAAEPTADDREAARSLAR
                     CVSIFVARLPSPYREAVTLVELEGLTVREAAEMGGVSVSGMKSRVQRGRAQLRRLFDE
                     CCEIALDARGKVIDYTPRLRPCPTCT"
     gene            complement(7958..8314)
                     /locus_tag="VF516_18405"
     CDS             complement(7958..8314)
                     /locus_tag="VF516_18405"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8109713.1"
                     /translation="MRMAQTIVFVDDVARMQAFYQGILGLPVITAEPGWVQLDAGGVL
                     FALHAIKPGPRLHDPPPERVDSHIKLCFHVDDVDAARAALVAAGVRMREVHRYSGVMF
                     CDGIDPEGNVFQITTR"
     gene            8418..9338
                     /locus_tag="VF516_18410"
     CDS             8418..9338
                     /locus_tag="VF516_18410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109714.1"
                     /translation="MRSAMVLIVIAGCGGGKAPEAARPAPAPLPAYASADPITSVRMF
                     APGVVSTAAPEFAIAFAPDGSTVYFDRASDDRSQLAIVASRFANGAWQPATPLPFSTG
                     EFRDVDPFVAGDRLYFSSNRPRPGDKDTDFDTWYVVRTGETWSQPVHVDGAPSGPGNQ
                     VCVSIARDGTLYFQSDLTGAGDIYRAARAGDGYPTAAPVAEITTPASESNPAISPDGT
                     LLVFVSDREGGQGGADLYASRARDGTWSPARNLGPAVNSRFADFAPAFSPDGKYLFFT
                     SERPGLVPTPASGARPPGDIYQIEVAALGI"
     gene            complement(9457..9762)
                     /locus_tag="VF516_18415"
     CDS             complement(9457..9762)
                     /locus_tag="VF516_18415"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8109715.1"
                     /translation="MQRHRFVLVLASIVGLAFLGCFKSSGAPAASSELKPLTVDQVAA
                     KIAANDGKTFVYDNNDKDSWVAGHVPGARWVDSEHVTAADLPSDKTATLIFYCHNET"
     gene            10004..11674
                     /locus_tag="VF516_18420"
     CDS             10004..11674
                     /locus_tag="VF516_18420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8109716.1"
                     /translation="MNGRATDRLPTGWRGYAWALFAVVFALLARWFADPRLGTEQLPY
                     GSVLVAATLIAWFVGFRPALVVIFAGGLGAHFFLIPPRWSFEPGNAHQTWGLVVFLFL
                     GNIISAFGGFARTQRARAEASAASTRRAEAALREAHAELERRVEERTAELAQTNESLR
                     ASEQRFRLLVEGIQGYAIVLVNEAGAVIAWNGGAERLFGRGADVVGEPFSTLIPGLDG
                     VALLEAARGGTAPCDALRRDGAMFPIELEVNRLDPPAAAAPRLASLAPSGMFIAIVHD
                     ISERKRLEALTEQRQRRADELRRKAEELAADNQRMQEASRLQSEFLANMSHELRTPLN
                     AIIGFADLLHGGMVGDLDTQQREFVGDILTSSRHLLQLINDVLDLAKVESGTLEVVPE
                     PIDLARVVGEVRDILRGLAIDRKLDLELQLADDLGSVIVDPAKLRQILYNYVSNALKF
                     TPEGGRVMIRAALAGDAFRIEVEDTGIGIASEDQRYLFLPFRQLDASPTKRYQGTGLG
                     LALTKRLAEAHGGRVGMHSARGVGSTFWVELPRVTPTPAAPVLRAAQR"
BASE COUNT         1914 a         3921 c         3993 g         1850 t
ORIGIN      
        1 ggggcctggt caaccgcgtc gtcccgcgcg ccgacctggt cgccgagacc cacaagctcg
       61 cgcgcgccgc cagccgcggc agcctgacct ccaaggcgct cggcaagcgc accttctacc
      121 agcagatcga gctcgacgtc gaggccgcct acgccgaggc catcgaggtc atggcgcaga
      181 gcgcggtcac tcccgacggc cgcgaaaaca tgcgcgcctt catcgagaag cgccacgcgg
      241 tctattcctc gacccggccg ccgacccggt agcacgcgcc gaccgttgcc cgggcaacgg
      301 cgcggatcgc tcgggggtta gggggatcga tggcgacagc cgaccagccc aacctttggc
      361 gagcccaggt tcgcccctgg tctcgccggt gaaagccggg ttgcgtcagc gcacctgacc
      421 agcacgaccc tactcgccgc caacgtcagc atccgcatcg atcgcgacct ccggcacctg
      481 cggcagcaac acggcaagga gcccggccgt ggccgcgaac gccgcggaag cccataggca
      541 tccgatgagc ccagctcgtt ggtacagcgc gccggagagc actgtccccg cgagccttcc
      601 ccccgcgttg gccatgtagt agaagccgac gttcatcgcg accttgtcgc catccgagta
      661 cgcgaggatc aggtacgagt gaaccgatga gttgatcgcg aacgcgacgc cgaacagccc
      721 gagccccgcc acgaccagga gctcgggctg gtaatcgtgc tcgacgccga cgatcacgcc
      781 gatgatcacc gccgcaagca cgaacgccag cacggtagct gaccgtccct gtggcgcgcg
      841 gcccttggtg aagcggcgca gcagcagcgg cgtgatcgac tggatgacac cgtagccgat
      901 cacccacgcc gccaggaagc cgccgacttc catgaagccc caggcgaacc gggtcgcgag
      961 gaaaaccggg gcaccgacga caaaccagat gtcacgcgcg ccgaacagga agaaccgcgc
     1021 cgccgagaga acgttgatcg cgtggttgtt cgagaagatg tggctgaact tcgctttcga
     1081 cttggccttg ccgatctcgc gcggcagcga cgcagccgag ccaatgagcg tgatggcgat
     1141 cccgccggcc atcgcgaaca gcgactcccg gaagccgagc gcaccgagca agaaccctcc
     1201 caagaagaag ccgatgccct tgagggcgtt cttcgaacca gtcagcaccg ccacccactt
     1261 gaacagcgac gactccgcac ccacagggac gagcaccttg attgcgctct ttgcgctcat
     1321 cttggtgaga tcctttgcga tgcccgaaag ggcttgcgcc gtcatcacgt acaccaccga
     1381 cgcagagcgt ccccacgtcg gttcgagcag cgcgagcagc gcgagtgcag cgacctggag
     1441 cgcgagcccg ccgagcaagg tcgcgcgaag cccgagacgt acggcgatcc agccacccac
     1501 gaggttcgtg accacgccga aaaactcgta gaacacgaac aggaacgcga gagcgaccgg
     1561 cgtatagccg aggcggtaga agtgcaagag tacgagcatc ctcagcgcgc catcggtgag
     1621 cgtgaacgcc cagtaggccg cggtgacgag gacgtagttg cgaaggctgc ccatggtgag
     1681 gttcagtgga gatgctttcc aaccttgcgt gcgagctcca tcatgcgatg gacatatccg
     1741 atctcgttgt cgtaccaggc gaggatcttc accgacgtgc catcgaccac catcgtcgat
     1801 gccgcgtcga tgatcgcgga gtgtgggtca cccttgaaat cgaccgagac gagctcgcgc
     1861 tcctcatagg ccatgatgct cttcagtggc ccctctgccg cgaccctgag caagtggttg
     1921 acctcctcga tggtcgtcgg ccgcgccgtc tcgaacacgc aatcggtcag cgaagcgttg
     1981 agcagtggca cgcggacagc gagcccgttc aacttgccct tgagctcggg atagatcagc
     2041 gtgatcgcag tcgccgagcc tgtgctcgtc ggaatcagcg acatgctgct cgcgcgagcg
     2101 cgccgcaggt ccttgtgcgg cgcatcgatc acgacctgcg tgttcgtgat gtcgtggatc
     2161 gtcgtgatga cgccgtggcg gatcccgatg ccctcatgga tcaccttgac gacgggcgcc
     2221 aggcagttgg tggtacacga cgccgccgtg acgaggtgat gcttgtcggg atcgtagaga
     2281 tggtcgttga tgccaacgac aatgttgagc gcaccctcct tcacgggcgc ggccacgatc
     2341 accttcttga cgccaccgtc gaagtacggc gcgagaagcg ccggggtccg gaacttgccc
     2401 gagcattcca gaacgagatc gacgccgtac ttcgtccagg gcactgcgcc cggcgtgcca
     2461 tgctcgctga acgtgagctg cttgccgtcg atgatgatgc catcgcgcgt cgtcttcaca
     2521 tcgttaggcc agcggccgtg cacggagtcg aacgacagca ggtgcgcagc gacctcgggg
     2581 ccaccctgga cctcgttgat gtgaacgaac tctacgtcgg gccagctcca ggctgcgcgg
     2641 agtgccaacc ttcccatcct gccgaagccg ttgatcccga tccggatgct catgtgcgtc
     2701 tcctctgggt cgtccttgca tggctatatc gcaatgtaag catgtaggct agcccatgac
     2761 gcgcacattg ctggcctgcc aggccccgga gggccgaacg actaccggct cggtcgatcg
     2821 agatgcttcg agcgtggcgc gcggctcgtg ggcgctacgt gttcacttgc aggcatctgg
     2881 accacatgac ttgcggagcc gcgcgtgatc ggcccgtagc gctcgctccg cgccgaacgc
     2941 cttggtcagc gctgcgagct ggctttcaac gagatcgtga tgctgcttgc tcagggaata
     3001 atagacccag gtcgcctcgc gccgagcatt gaccaggccg gccatgcgca agatcccgag
     3061 ctgccgtgag acgttaggct ggctgagatc gagcgccttc tccaggtgac atacgcacag
     3121 ctcaccgtgc gtgagcaacg cgacgattcg caaccgggtc tcgtcgccaa gtgcgcgaaa
     3181 ggcgagcgag agcggccgaa catctagttc cgatgcgcta gcgagcacgg gacctgtata
     3241 tcacgccttg cgcatatctt cgagctgtgc cggatcgacc cgccgctgct cttacgtcgc
     3301 tgactccccg gcggggtaag cgcttgcgat cgccgtgctt gcagcgacgc cgaccgctcc
     3361 gaacagggcg acccacgacc gctccaggag ggcggagcta acaagccagc ggtcgacgac
     3421 gatcgccacg ccgcagaaca gcaagctgcc gaacgcttct cgaacgagac tggccccggg
     3481 gagcagttac aacgggcctg gcagctggtc ggtcgcaggg cttgtcatgg tcactatatt
     3541 ctcatatacg tatgtcgcga tgcaacgacc acgcaggcag cacggcacgt tcttgcttcg
     3601 attcgctact cggccaggtg acatctttgc agcaatgcag gatgccgacg tggcctggtt
     3661 cctggcggta catgacgagg ccagacgccc cacgattccg ccgatgcacg tggttcgccg
     3721 cctcaactcg gtggcgctga cggcgcttgc attgatggcc agtgtgagtg cgtgtgatcg
     3781 ccgcggggag cgcgacgagg gcgtgcgctt gagcaatgca ccaccggtgc gcgtcgatgg
     3841 gtcgagcacg gtctatctga tctcgaaggc ggtcagtgag gagctcgcca agcagcacgg
     3901 ccccagctcg acggtgggcg aatccgggac caccggcggc ttccggaagt tctgccacgg
     3961 cgacatcgat gtcaccggcg cgcgccgcaa cgatggcgtc accgagcgcg ctctctccgg
     4021 gctgcgccgc cacggcgcag ctctgcgacg aagccatcgc ggccggccag cggcgcgctg
     4081 cgagccgaat ttgcgacgca aagcaacagg atgctcctgg atgcggcggt catggtcacg
     4141 cgttggacgc gacggtcggc gcgctatcgt ttgctcggga tggggagacg gcctcgatcg
     4201 cggcgctcgg gtagaatcgt cgctgcgccc acagcgccac gttcaccagg ccgatcaggg
     4261 caggcacctc gacgagcggg ccgatcaccg ccgcgaacgc cgccccgctg tgaatgccga
     4321 agctcgcgat cgccacggcg atggccagct cgaagttgtt ggatgcggcc gtaaaggaca
     4381 gcgtggcgga ctgcctgtag ttggcgccga cgcgcttgct catcacgaac gagacggtga
     4441 acatcaccac gaagtagatc agcagcggaa cagcgatgcg gagcacgtcg agcggcagct
     4501 gcacgatggc ctcgcccttg agcgagaaca tcacgacgat cgtgaacagc agcgagacga
     4561 gcgtgatcgg tccgatgcgt gggacgaacc gcttctcgta ccagtcgcgt ccgcgggcgc
     4621 cgacgagcac gcggcgcgtg atgaaccccg ccgcaaacgg gatgccgagg tagatcgcga
     4681 cgctcttagc gatctcgcgg atcgtgatgt ccacgatcgc gctctggagg ccgagccagc
     4741 gcgggagcag tgtggcgaag aagtacgcgt agaccgagaa gaacagcacc tgaaagatcg
     4801 agttgaacgc gacgagcccg gcgcaatatt cggtgtcgcc cttggccagg tcgttccaga
     4861 cgatcaccat cgcgatgcag cgcgcgaggc cgatcaggat gaggccgagc atgtactccg
     4921 ggtggtcacg gaggaacacc acggcgagcc cgaacatgag cagcggtccg atgatccagt
     4981 tctgcacgag cgagagcagc aagacgcgcc ggttgcggaa caccttgccg agctcctcat
     5041 agcggacctt cgccagcggc gggtacatca tcaggatgag gcccacggcg atcgggatcg
     5101 acgtcgtgcc aatgctcaag cgattcagcg cgctcgtgac cccgggcgcg acgaatccga
     5161 tcgcgatgcc gagcgccatc gcggcaaaga tccacagcgt gagatagcga tcgaggaacg
     5221 agagcttgct ggcgatgcca gtcctgctca tgtgcatagg cttgatctca gcgtcggccg
     5281 cgggtggtgg acggaagtgg cggtgcgcga ccgcgccagc agcatcacga cgcacacgcg
     5341 tcgggaccgc acgacctgcg cagcttggcg tgattggcgc gcagggcgcg ctcggccccg
     5401 aacgccttgg tcagcacatc gagcgccttg gcaaccgtgt cgtgctcctg gtcggtgagc
     5461 cggtagtaga cccaggtgcc ctcgcggcgg cagtcgacga cgcccgcggt ccggaggatg
     5521 ccgaggtggc gagacgcggt cgactggttg aggtcgagcg cggcctcgat gtggcagacg
     5581 cagagctcgc cgtgcgatag cagcgcgacg atgcgcagcc gcgtctcgtc gccgagggcg
     5641 cggaacagcc gcatcagggg ccgaacgtcc agagcgttcg tgctggacaa catgacagtg
     5701 ctatatcacg atgtccgcat gtataccagc ctgctgtttc tgtgcgtcgc gaactccgcc
     5761 cggtcccaga tggcggaagg cctggcgcgc gcgctgttcg ggggcctggt gcaggttcag
     5821 agcgcgggca gccagccaac ccgggtcgac ccgaacgcga tcgcggcccc ggcatgaggt
     5881 ccggtcgatt tcctgcgtcc ttttttcccc aagcccgtct cccgggcagg aggttacgat
     5941 gtccgagatg aaggccgatg acgtgcggac ggtggtccgc gagcactacg ggaacatcgc
     6001 acgcggtgcc ggcacgagct gcgcgccggg atgctgcagc tccgcgggga ccgcggcggc
     6061 gagcctgggc tacaccgccg atcagacgga ggccgtcccc gacggcgccg atctggggct
     6121 cggctgcggc aacccgacgg cgatcgcctc gctgcgcgac ggcgagacgg tgctcgacct
     6181 gggtgccggt ggcgggttcg actgcttcat cgccgcgcac caggtcgggc cgcgcggccg
     6241 ggtgatcggt gtcgacatga ccgcggacat ggtggcccgt gcccgcgcca acgcgcgcaa
     6301 ggtgaacgct accaacgtcg agttccggct cggcgagatc gagcacctgc cggtcgccga
     6361 tgcctcggtc gacgtgatcc tgtccaactg cgtgatcaac ctgtcgccgg acaagcccgc
     6421 ggtgttccgc gaggcgttcc gcgtgctgcg cgccggcggg cgcctggcga tcagcgacgt
     6481 cgtggcgacc ggcccgatcc cggtcgagct gcagaaccag gccgcggcgc tcgccgggtg
     6541 cgtcgccggt gcggcgccgc tggacgacgt gcgcgcgatg ctcgctgcgg ccgggttcgt
     6601 ggacgtcgag gtgaagatct caccgcgcag cgccgagatc gttgggtcgt ggctgccggg
     6661 gatcgagcgg ttcgtggcgt cggcgacgat cgaggcgcgt aagccgggcg gtgacgccgg
     6721 tgcatcgtcg tgctgtggcc cggagtgctg cgcatgaacg catcgcctgc gccttggcaa
     6781 gacgtcgcgg cgcagctccg gccgttcctt gcccggcggg tcgccgcggc ggacgtcgat
     6841 gacgtcctgc aaaacgtctg gctccgcgtc cagcgcgggc tcccagggct tcgagacgag
     6901 gagcggttca cctcgtggct gtttcagatc gcgcgtagcg ccgtggccga gcaccagcgc
     6961 gcccgcgcgc gccatccgct gcccgatgcg cccgcggagt cagacctggc ggccgagcct
     7021 accgccgatg atcgcgaggc cgcgcgctcg ctggcgcgct gcgtctcgat cttcgtggcg
     7081 cgcttgccgt cgccctaccg ggaagccgtg acgctggtgg agctcgaagg gctcaccgta
     7141 cgcgaggcgg ccgagatggg cggggtctcg gtctcaggga tgaagtcgcg cgtacagcgc
     7201 gggcgcgccc agctccgccg gctgttcgac gaatgctgtg agatcgcgct cgatgcgcgc
     7261 ggcaaggtca tcgattacac gccccggctg cgtccctgcc cgacgtgcac ctgatccgac
     7321 gcggaggctt ccgatgtccg cactccgcaa gcccaaccgc gatggcttcg tgagtcgcgg
     7381 tcccaacgcg gccgatgtga caccctgggg ctgtcgcggc cgcctgagcg cggccggatc
     7441 cggtgttgca tcgcgcacgg gcggcgctag aagcgtcgtg acgccggtct tgagcagatc
     7501 gccgtggcgc tgcgggacgt gaccgtgcgg tgcggcacca ggcggtcgag ttggaccgtc
     7561 tcgccgacgg tgagctccac gatgctcggt tcccgaacct ggagcgtcac tttgcgcggt
     7621 tcctcattcg atactccttg ctgatttgtg ttgtggacta ctcggtggct cctaccatct
     7681 gaccaatccg cagtacgaac atggccatat gaatacccaa ttcgcattca tggctcgggc
     7741 ccaccagcga gaagccatcc atccgccgat atagcggttt atcacagcat cagctgattg
     7801 gcgatcataa gattttgcat gttggcctgc aacgcggtgt tccggtgccg cagcggcgcg
     7861 atgtaccggt cacatcgcca tcgtcatgtg gatcgctggc ggcgccttct tgcgtgggcc
     7921 gaccgtcacg cggccgtcga cgtctgagac caatcgctca gcgcgtcgtg atctggaaga
     7981 cgttgccctc gggatcgatg ccgtcgcaga acatcacgcc gctgtaacgg tggacctcgc
     8041 gcatccggac cccggcggcc accagggcgg cgcgcgcggc gtcgacgtca tcgacgtgga
     8101 agcacagctt gatgtgcgaa tcgacgcgct cgggcggtgg atcgtgcagc ctcggcccgg
     8161 gcttgatcgc gtgcaacgca aagaggacgc ccccggcgtc gagctgcacc cagccgggct
     8221 ccgcggtgat caccggcagc ccgaggatgc cctggtagaa cgcctgcatc cgcgcgacgt
     8281 cgtcgacgaa gacgatggtc tgtgccatgc gcatgcgcga agcttttcat acttccgcac
     8341 gccgcgccgc acggtgtacc gcccgcgggt cgcgcgccct gttccggtcc gggccgcgat
     8401 ctcggatacg atgcggcatg cgcagcgcga tggtcctgat cgtgatcgcg ggatgcggcg
     8461 gcggcaaggc gccggaggcc gcccgtccgg caccggcccc gcttcccgcc tatgccagcg
     8521 ccgatccgat cacgagcgtc cgcatgttcg cgccgggggt ggtgtcgacc gcggcgccgg
     8581 agttcgcgat cgcgttcgcg cccgatggct cgacggtgta cttcgatcgc gcgagcgacg
     8641 atcgcagcca gctcgcgatc gtcgcctcga ggttcgcgaa cggcgcctgg cagccggcga
     8701 cgccgctgcc gttctcgacc ggcgagttcc gggacgtcga tccgttcgtc gcgggcgacc
     8761 gcctgtactt cagctcgaac cgtccgcgac cgggcgacaa ggacaccgac ttcgacacct
     8821 ggtacgtggt ccggaccggc gagacctgga gccagccggt ccacgtcgac ggcgcgccga
     8881 gcgggcccgg caatcaggtg tgcgtgtcga tcgcgcgcga tggcacgctg tacttccagt
     8941 ccgacctcac cggcgctggg gacatctacc gcgcggcgcg cgccggcgac ggatacccca
     9001 ccgccgcgcc ggtcgcggag atcacgaccc cggcgagcga gagcaacccg gcgatctcgc
     9061 cggacggcac gctgctcgtg ttcgtcagcg accgcgaggg cggccagggc ggcgcggacc
     9121 tctacgccag ccgcgcgcgc gatggcacat ggtcgcccgc caggaacctc ggccccgccg
     9181 tcaactcgcg gttcgccgac ttcgcgcccg cgttcagccc cgacgggaag tacctgttct
     9241 tcacctcgga gcgccccggc ctcgtgccga ccccggcgag cggcgcccgc ccgccgggag
     9301 acatctacca gatcgaggtc gcggcgctgg gcatctgatt cagcccttct cggtcggctt
     9361 gccagcgctc ttccagccca cgatgccctg cggcatgatg tagacgttcg cgtaaccgag
     9421 cttctccgcc gcgaccgcgg cggtgtggca ggcgtgtcaa gtctcgttgt gacagtagaa
     9481 gatcagcgtc gccgtcttgt ccgagggcag atccgcggcg gtgacgtgct cgctgtcgac
     9541 ccacctggcg cccggcacgt ggccggcgac ccagctgtcc ttgtcgttgt tgtcgtagac
     9601 gaaggtcttg ccgtcgttgg cggcgatctt cgcggccacc tgatcgacgg tgagcggctt
     9661 gagctcgctc gacgcggcag gtgcgccgct gctcttgaag cagccgagga atgcgaggcc
     9721 gacgatgctg gcgaggacga ggacgaagcg gtggcgttgc atggacagat gctacatcgg
     9781 cggcccgatg atcaggactg ggctcggatc ccagatcagg agttttctgg tgtgcggtga
     9841 tcgggtcgac gtgcgccagc gcctcgcatg agccgctggc cagttagtct gccgcggaat
     9901 gcggcagtcc gtcgaggtgt gtgcacggaa tgatccattc tgctcgccgg ggctgtgatg
     9961 cccgcgctac cctcgcgatg ggtatgtacg cagcgtggga cgtgtgaacg ggagagccac
    10021 ggaccggctt cccaccggat ggcgcggcta tgcctgggcg ctgttcgcgg tggtgttcgc
    10081 gctgctggcg cggtggttcg ccgacccgcg gctcggcacc gagcagctgc cgtatggctc
    10141 ggtgctggtc gccgcgacgc tgatcgcgtg gttcgtgggc ttccggcccg cgctggtggt
    10201 gatcttcgcc ggcggtctcg gtgcccactt cttcctgatc ccgccgcgct ggagcttcga
    10261 gccgggcaac gcgcatcaga cctggggcct ggtggtgttc ctgttcctcg gcaacatcat
    10321 ctcggcgttc ggggggttcg cccgcaccca gcgcgctcgc gccgaggcgt ccgcggcgtc
    10381 gacacgccgg gccgaggccg cgctgcgcga ggcccacgcc gagctcgagc gccgggtcga
    10441 ggagcgcacg gcagagctgg cgcagaccaa cgagtcgctg cgcgcgtccg agcagcggtt
    10501 ccggctgctg gtcgagggca tccagggcta cgcgatcgtc ctggtgaacg aggccggcgc
    10561 ggtcatcgcg tggaacggcg gcgcggagcg gctgttcggc cgcggggcgg acgtcgtggg
    10621 cgagccgttc tcgacgctga tccccgggct cgacggcgtg gcgctgctcg aggccgcgcg
    10681 cggcggcacc gcgccgtgcg acgcgctccg gcgcgacggt gcgatgttcc cgatcgagct
    10741 cgaggtcaac cggctcgacc cgccggcggc cgctgccccc cgcctcgctt cgctcgctcc
    10801 gagcgggatg ttcatcgcga tcgtgcacga catctccgag cgcaagcggc tcgaggcgct
    10861 gaccgagcag cggcagcgcc gcgccgacga gctgcgccgc aaggccgagg agctcgcggc
    10921 ggacaaccag cggatgcagg aggcgtcgcg gctgcagagc gagttcctcg ccaacatgag
    10981 ccacgagctg cgcacgccgc tcaacgcgat catcgggttc gccgacctgc tgcacggcgg
    11041 catggtcggc gatctcgata cccagcagcg cgagttcgtc ggcgacatcc tgaccagctc
    11101 gcgccacctg ctgcagctga tcaacgacgt gctcgacctc gccaaggtcg agtctggcac
    11161 gctggaggtg gtccccgagc cgatcgacct ggcgcgcgtg gtcggcgagg tccgcgacat
    11221 cctgcgcggc ctggcgatcg accgcaagct cgacctcgag ctccagctcg ccgacgacct
    11281 gggcagcgtg atcgtcgacc ccgccaagct gcgccagatc ctctacaact acgtgtcgaa
    11341 cgcgctgaag ttcacgcccg agggcggccg cgtcatgatc cgcgccgcgc tggccggcga
    11401 cgcgttccgg atcgaggtcg aggacaccgg gatcgggatc gcgagcgagg accagcgcta
    11461 cctgttcctg ccgttccgcc agctcgacgc gagcccgacc aagcgctacc agggcaccgg
    11521 cctgggcctc gcgctgacca agcggctcgc cgaggcgcac ggcgggcggg tcggcatgca
    11581 cagcgcgcgc ggcgtcggca gcacgttctg ggtcgagcta ccgcgggtga ccccgacccc
    11641 ggccgcgccg gtgctgcgag ccgcgcagcg ctagctcg
//
LOCUS       DASVOR010000949         2199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_506131,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000949 DASVOR010000000
VERSION     DASVOR010000949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506131"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2157)
                     /locus_tag="VF516_18425"
     CDS             complement(<1..2157)
                     /locus_tag="VF516_18425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109717.1"
                     /translation="MRTALRTTWWIGLACWLWAWAAPARAAPPEPSGPHPRMLLDAEL
                     RAAWRARSKEGHGPIVGSIAICDGDRSPREHAAAQYMGSEWKKMLQACLVAWAATGEQ
                     SHARTALRYFTALIDDLDTVGDGKGGDVAANRDAGYAIRNLGPYTALAYDWLHDAPGM
                     TPELQARARRRWAAWLAWFERSGYHPHDPGSNYHAGYLLAATMIAIAQAGEAGDAGRA
                     LWRDVADRMWGKEMAAAWAPGGPLDGGDWDEGWQYGPLAVAEYALGARIVRAHGIRVD
                     GATGWLDSVLRRHVYALTPADRLWAGGDFDSEAAYMEPSAMSLAAIALGDAPPLTRRW
                     AKGELSRLKLGDQDSLLYDALAAIGESPVVVPRASWPTWYRAAGTGTLFTRTSWDPHA
                     IWFVAECGRTTATDHHSPNAGNFALSRGAADLIVDPSPYGSLSTLTGNAPTVRSPQLP
                     GSYQPSQGPWGAAVRWRWATRTTSGVVAARCDYSDAFRFQDRPSDVPEALRDFVLVPG
                     RDGRDAQLVIVDRAVTRDIDRPMYLRFRVPGPLTIDAAGTATAEIGGARLAIHGPAGK
                     LGKTALKDCFQPGTERGNCDAARVPVTDYRAEIPGPEPRAVHVIDATGPRAGATSQPI
                     SGPGWTGMALTGPRDAVVVWPTRDGALSYRAPRRPAISHVVLDAPATGGMVHLTARPD
                     GDACEVTVEPGGNLPAAPLVAVLDERCNVAADPPASD"
BASE COUNT          253 a          808 c          857 g          281 t
ORIGIN      
        1 gtcggaggcg ggcgggtcgg cggcgacgtt gcagcgctcg tcgagcacgg ccaccagggg
       61 cgccgcgggc aggttgccgc cgggctcgac ggtcacttcg caggcgtcgc cgtcggggcg
      121 cgcggtgagg tggaccatgc cgccggtcgc cggcgcgtcg agcacgacgt ggctgatcgc
      181 gggtcggcgc ggcgcgcggt acgacagcgc gccatcgcgg gtcggccaca ccacgacggc
      241 gtcgcgcggg ccggtcagcg ccatgccggt ccagccgggg ccggagatcg gctggctggt
      301 cgcgccggcg cgcggtccgg tcgcgtcgat cacatgcacc gcgcgtggct ccgggccggg
      361 aatctcggcg cggtagtcgg tgaccgggac ccgcgctgcg tcgcagttgc cgcgctcggt
      421 gccgggctgg aagcagtcct tgagcgcggt cttgcccagc ttgccggccg ggccgtggat
      481 cgccagccgc gcgccgccga tctcggcggt cgcggtcccg gccgcgtcga tcgtgagcgg
      541 gcccgggacg cggaaccgga ggtacatggg gcggtcgatg tcgcgggtga cggcgcggtc
      601 gacgatcacg agctgggcgt cgcggccgtc gcggccgggc acgagcacga agtcgcgcag
      661 cgcctcgggc acgtcgctcg ggcggtcctg gaaccggaac gcgtcgctgt agtcgcagcg
      721 cgcggcgacc acgccgctgg tggtccgggt cgcccatcgc cagcgcaccg ccgcgcccca
      781 cgggccctgg ctcggctggt acgacccggg cagctgcggg gatcgcacgg tcggcgcgtt
      841 gccggtcagg gtcgacagcg agccgtacgg cgacggatcg acgatcaggt cggcggcgcc
      901 gcgcgacagc gcgaagttgc cggcgttggg gctgtggtgg tcggtggcgg tggtccgccc
      961 gcactcggcg acgaaccaga tcgcgtgcgg gtcccagctg gtgcgggtga acagggtccc
     1021 ggtcccggcg gcgcgatacc aggtcggcca cgacgcgcgc ggcaccacca ccggcgactc
     1081 gccgatcgcc gccagcgcgt cgtagagcag cgagtcctgg tcgccgagct tgagccgcga
     1141 cagctcgccc ttggcccacc gccgggtcag cgggggggcg tcgccgagcg cgatcgccgc
     1201 cagcgacatc gccgacggct ccatgtacgc cgcctcgctg tcgaagtcgc cgccggccca
     1261 cagccggtcg gccggggtca gcgcgtagac gtggcggcgc agcacgctgt cgagccagcc
     1321 ggtcgcgccg tcgacccgga tgccgtgcgc ccggacgatg cgcgcgccga gcgcgtactc
     1381 ggcgaccgcg agcggcccgt actgccagcc ctcgtcccag tcgccgccgt cgagcgggcc
     1441 gcccggcgcc cacgcggctg ccatctcctt gccccacatc cggtcggcga cgtcgcgcca
     1501 cagcgcgcgg ccggcgtccc ctgcctcgcc ggcctgggcg atcgcgatca tcgtcgccgc
     1561 gagcaggtag ccggcgtggt agttgctgcc ggggtcgtgc gggtggtagc cgctgcgctc
     1621 gaaccacgcc agccaggccg cccagcgccg ccgggcacgg gcctgcagct cgggcgtcat
     1681 gccgggcgcg tcgtgcagcc agtcgtaggc cagcgcggtg tacgggccga ggttgcggat
     1741 cgcgtagccg gcgtcgcggt tggcggccac atcgccgccc ttgccatcgc cgacggtgtc
     1801 gaggtcgtcg atcagggcgg tgaagtagcg cagcgcggtc cgggcgtggc tctgctcgcc
     1861 ggtcgcggcc catgccacca ggcaggcctg cagcatcttc ttccactccg agcccatgta
     1921 ctgcgccgcg gcgtgctcgc gcgggctgcg atcgccgtcg catatcgcga tcgagccgac
     1981 gatcgggccg tggccctcct tgctccgcgc ccgccaggcc gcgcgaagct cggcatcgag
     2041 cagcatgcgg gggtgcggcc cgctcggctc gggcggtgcc gcgcgggccg gggccgccca
     2101 cgcccacagc cagcatgcaa ggccgatcca ccaggtcgtt cgcagtgcgg tgcgcacccg
     2161 agaaaacgta gcatccggta tgcccgcaca cgtccggca
//
LOCUS       DASVOR010000950         4098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_301845,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000950 DASVOR010000000
VERSION     DASVOR010000950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301845"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..762)
                     /locus_tag="VF516_18430"
     CDS             complement(115..762)
                     /locus_tag="VF516_18430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEX8109718.1"
                     /translation="MADTATRAGERPATSAILDHVNRVHAPHDAGLARAFAVPDGVPP
                     IQVSPSDGKLIELLLRLVGAAKVVELGTLVGYSAIHMARALAPGGHLWSIEYEPRHAE
                     IARANLEAAGVADRVTVLVGAGRKVLPTLVDHAPFDAVFIDADKVSYAHYGRWAIDHL
                     RQGGLVLGDNAYLFGELLDDSDRGRAMRAFHELVAAHCDSVCATTPEGLVIGVKR"
     gene            complement(775..1983)
                     /locus_tag="VF516_18435"
     CDS             complement(775..1983)
                     /locus_tag="VF516_18435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidogalycan biosysnthesis protein"
                     /protein_id="HEX8109719.1"
                     /translation="MKIAVRAVASVGDFDAAAWDALGHGASPFLRHGFLRALEESGSV
                     DPATARRRRSGWSSLYLLAEQAGRLVGAVPAFLKDHSYGEYIFDWSWASAAQRAGLRY
                     YPKLVIAAPATPATGHRLLIAPGADAAAVRAALIEAVRAVAEDTECSSIHWLFCTAEE
                     QAHLTEHGFFPRASMQFHWHNQDYTTFDDFLAQLKSRKRKQLRKERARARGAIERLAW
                     VPGGELSEAQLDDLDRFYRNTTDQHGGRDYLRPGFFHALARHLPDAMRMVEVLAGGKR
                     VAGALFLETDRALYGRYWGADVHVDLLHFETAYYAGIERAIARKLPLFEAGAQGEHKL
                     LRGFDPAPTYSAHWIRHPGLAAAIEDYTRREAAAVTAQIAEAAKFGPYRRAGGDDDEP
                     GANDEPGADC"
     gene            2010..2939
                     /gene="grxD"
                     /locus_tag="VF516_18440"
     CDS             2010..2939
                     /gene="grxD"
                     /locus_tag="VF516_18440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEX8109720.1"
                     /translation="MTLDPETRKKIEHLVASDSVVLFMKGSRSFPQCGFSASVVNILN
                     TLIPKYTTVNILADADVRTGMKEFSDWPTFPQLFIRGEFVGGADIVRQMHESGDLENK
                     LGDLVAPAKAPSVAVSPRAAQELAEALKEGSPGDVIHLTITPSWEHQLDLGAREASHV
                     TVQSAGITVQLDRASAGRAEGVTIDFIEASDGAGFKIDNPNRPAMVKEIAPRELKALL
                     DSGRVTHFYDVRPDKERSIAKIAGVRQLDDAAMAAIEALPKDTPLAFHCHHGGRSRGA
                     AEHFLKLGFKNVYNMAGGIEAWSRDVDPSVPRY"
     gene            complement(2955..3314)
                     /locus_tag="VF516_18445"
     CDS             complement(2955..3314)
                     /locus_tag="VF516_18445"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8109721.1"
                     /translation="MDIQRYEVGPRMSQAVVYGGLAYLAGIVADDLVPSVADQTRQIL
                     AKIDRLLAMAGSDKTRILKANIWLSDIRRFDEMNAVWDGWVAQGHPPARATVEARLAG
                     PGYLVEIMVEAAVAAAG"
     gene            complement(3383..>4098)
                     /locus_tag="VF516_18450"
     CDS             complement(3383..>4098)
                     /locus_tag="VF516_18450"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8109722.1"
                     /translation="GVAARRAPGTTEDLTATPPWTFDALAGAGAVVSSARDLPRLIDA
                     ELDAVDGSTQPLRHAMKLTQEPQLDRTGDNVSLGWLIDSAGRYWRNGGTGGYHAFIGF
                     DPKTKRGVVLLASTATSTIERLAEPMYKVLEGSPPPPAKLPGPAELAPLAGSYDLGGT
                     TLQVIVEGKRLYLAGPGEPRHRLSPIGDREFWLESLQSLAVFEKEGDKVARVVFGIGD
                     RRVVAPRVDAKPDAKPAPK"
BASE COUNT          573 a         1482 c         1481 g          562 t
ORIGIN      
        1 ccgccgccga cgtgatcggc cggccgatcc tcgccgccct gcccggccgg tcgcagcggc
       61 tcgccggcgc cgcgctcggc gtcgcgctgg tgatcgcgag cctgctccag ctgctcagcg
      121 cttgacgccg atcaccaggc cctcgggcgt ggtcgcgcac accgagtcgc agtgcgccgc
      181 gaccagctcg tggaacgcac gcatcgcgcg gccgcgatcg ctgtcgtcga gcagctcgcc
      241 gaacaggtac gcgttgtcgc cgagcaccag cccgccctgc cgcaggtgat cgatcgccca
      301 ccggccgtag tgggcgtagc tgaccttgtc ggcgtcgatg aacaccgcgt cgaacggcgc
      361 gtggtcgacc agcgtcggca gcaccttgcg gccggcgccg accagcacgg tcacccggtc
      421 ggccaccccg gcggcctcga ggttggcgcg cgcgatctcg gcgtggcgcg gctcgtactc
      481 gatcgaccac aggtggccgc ccggggccag cgcgcgcgcc atgtggatcg ccgagtagcc
      541 gaccagcgtg ccgagctcga cgaccttggc ggcgcccacc aggcggagca gcagctcgat
      601 cagcttgcca tcgctcgggc tgacctggat cggcgggacg ccgtcgggca ccgcgaacgc
      661 gcgcgccagg ccggcgtcat ggggcgcgtg gacgcggttg acgtggtcga ggatcgcgga
      721 ggtcgcgggg cgctcgccgg cgcgcgtggc ggtgtcggcc atggtgagcg gactctagca
      781 gtcggcgccg ggctcgtcgt tggcgccggg ctcgtcgtcg tcgccgcccg cgcggcgata
      841 cggtccgaac ttcgcggcct cggcgatctg cgcggtgacc gccgcggcct cgcgccgggt
      901 gtagtcctcg atcgccgcgg ccaggccggg gtggcggatc cagtgcgccg agtaggtcgg
      961 cgccggatcg aagccgcgga gcagcttgtg ctcgccctgc gcaccggcct cgaacagcgg
     1021 cagcttgcgc gcgatcgcgc gctcgatgcc ggcgtagtag gcggtctcga agtgcaggag
     1081 gtcgacgtgg acgtcggcgc cccagtagcg gccgtacagc gcgcgatcgg tctcgaggaa
     1141 cagcgcgccg gcgacgcgct tgccgccggc aagcacctcg accatgcgca tcgcgtcggg
     1201 caggtggcgg gcgagcgcgt ggaagaaccc cggccgcagg tagtcgcggc cgccgtgctg
     1261 gtcggtggtg ttgcggtaga accgatcgag gtcgtcgagc tgcgcctccg agagctcccc
     1321 gcccggcacc cacgccaggc gctcgatcgc gccgcgcgcc cgcgcccgct ccttgcgcag
     1381 ctgcttgcgc ttgcgcgact tgagctgtgc gaggaagtcg tcgaaggtgg tgtagtcctg
     1441 gttgtgccag tggaactgca tgctggcgcg cgggaagaag ccgtgctcgg tgagatgcgc
     1501 ctgctcctcg gcggtgcaga acagccagtg gatcgacgag cactcggtgt cctcggcgac
     1561 cgcccggacc gcctcgatca gcgcggcgcg gaccgcggcg gcgtcggcgc ccggtgcgat
     1621 cagcaggcgg tggccggtcg ccggggtcgc cggggcggcg atcaccagct tggggtagta
     1681 gcgcaggccg gcgcgctgcg ccgcgctcgc ccagctccag tcgaagatgt actcgccgta
     1741 gctgtgatcc ttgaggaacg ccggcacggc gccgaccagc cggcctgcct gttcggccag
     1801 caggtacagg ctcgaccacc cggagcgccg ccgcctggcc gtcgccgggt ccaccgaccc
     1861 cgactcctcg agcgcgcgca agaagccgtg gcgcaggaac ggcgatgcgc cgtggccgag
     1921 cgcgtcccat gcggcggcgt cgaagtcgcc caccgaagcc accgcgcgga cggcgatctt
     1981 catgctagaa gaagccacga aaggccacca tgacgctcga ccctgagacc cgcaagaaga
     2041 tcgaacacct cgttgcgagc gactccgtcg tcctgttcat gaagggcagc cgaagcttcc
     2101 cgcagtgcgg cttctcggcg tccgtcgtga acatcctcaa taccttgatc cccaagtaca
     2161 cgacggtcaa catcctggcc gacgcggacg tccgcacggg gatgaaggag ttctccgact
     2221 ggccgacgtt cccgcagctg ttcattcgcg gcgagttcgt cggcggcgcc gacatcgtgc
     2281 ggcagatgca cgagtccggc gatctcgaga acaagctcgg cgacctggtc gccccggcca
     2341 aggccccgtc ggtcgcggtg tcgccgcgcg ccgcccagga gctcgccgag gcgctcaagg
     2401 agggcagccc gggcgacgtg atccacctga cgatcacgcc gagctgggag caccagctcg
     2461 acctcggcgc ccgggaagcc agccatgtca ccgtgcagtc cgccggcatc accgtgcagc
     2521 tcgatcgcgc gagcgccggc cgcgccgagg gcgtgacgat cgacttcatc gaggcgtccg
     2581 acggcgccgg cttcaagatc gacaacccca accggcccgc catggtcaag gagatcgcgc
     2641 ccagggagct caaggcgctg ctcgacagcg gccgggtcac ccacttctac gacgtgcgcc
     2701 ccgacaagga gcgcagcatc gccaagatcg cgggggtgcg ccagctcgac gacgccgcga
     2761 tggccgcgat cgaggcgctg cccaaggaca cgccgctggc gttccactgc caccacggcg
     2821 ggcgcagccg cggcgccgcc gagcacttcc tcaagctcgg gttcaagaac gtctacaaca
     2881 tggccggcgg catcgaggcg tggtcgcgcg acgtcgaccc cagcgtgccg cgctattgat
     2941 cgcggcccgc tctgtcatcc tgcggccgcg accgcggcct cgaccatgat ctcgaccagg
     3001 tagcccgggc cggccaggcg ggcctcgacg gtcgcgcgcg ccggcgggtg gccctgggcg
     3061 acccagccgt cccacaccgc gttcatctcg tcgaaccggc ggatgtcgga cagccagatg
     3121 ttggccttga ggatccgggt cttgtcgctg ccggccatcg ccagcagccg gtcgatcttg
     3181 gcgaggatct gccgggtctg gtcggcgacc gacggcacca ggtcgtcggc gacgatgccg
     3241 gccaggtacg ccaggccgcc gtacacgacc gcctggctca tgcgcggacc cacctcgtag
     3301 cgctggatgt ccatggcgca tggcgtaggc cggtccgccg ccgggtgcaa ggcacaccga
     3361 ccacagacgc ggtgcagcac agctacttgg gcgcgggctt ggcgtcgggc ttggcatcga
     3421 cgcgcggtgc gacgacccgg cgatcgccga tgccgaacac gacgcgcgcg accttgtcgc
     3481 cttccttctc gaacaccgcg aggctctgca gcgactcgag ccagaactcg cgatcgccga
     3541 tcggcgacag ccggtggcgc ggctcgccgg gccccgccag gtacagccgc ttgccctcga
     3601 cgatcacctg cagcgtggtg ccgcccaggt cgtagctgcc ggcgagcggc gcgagctcgg
     3661 cggggccggg cagcttggcc ggcggcggcg gcgagccctc gagcaccttg tacatcggct
     3721 cggccagccg ctcgatcgtc gaggtcgcgg tcgaggccag cagcacgacg ccgcgcttgg
     3781 tcttggggtc gaagccgatg aacgcgtgat acccgccggt gccgccgttg cgccagtagc
     3841 ggccggcgct gtcgatcagc cagcccaggc tgacgttgtc gccggtgcga tcgagctgcg
     3901 gctcctgcgt gagcttcatc gcgtggcgca gcggctgggt gctaccgtcg acggcgtcga
     3961 gctcggcgtc gatcagccgg ggcaggtcgc gcgcgctcga caccaccgcg ccggccccgg
     4021 cgagcgcgtc gaacgtccac ggcggcgtgg cggtcaggtc ctcggtcgtg cccggggcgc
     4081 ggcgcgccgc gacccccg
//
LOCUS       DASVOR010000951         3387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_38775,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000951 DASVOR010000000
VERSION     DASVOR010000951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38775"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VF516_18455"
     CDS             complement(<1..1393)
                     /locus_tag="VF516_18455"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX8109723.1"
                     /translation="MKSTAAAGDPAPARPTFTVFAVAEVRGQIGPCGCTTDPLGDLSR
                     TAQLVVSARAAGPVLFVDAGSLLYAHAPTPPQLAVQEELKADLLARTYQKTLLADAVG
                     LGPADLPNGAASLDHLRLARVVANAPPAGGPTGIAGPAKLIDVGGAKAGVFGVIAPGA
                     VAGLSLSDPVAAGKQAVAQLRGQGAQVVIGLVQAESKKDAVKLVRDIGGIDLAIAGLG
                     LAAPEPERIESEPQKVGDGWLVVPANRGQIVARFEVTLRGPGPLADAIGPGAAAAKLA
                     VIDRQLAALDADLKAFASDRSADPAFVAQKQKERAQLAADRDRLKTHPLVVPPHGSYF
                     TLDQVRIHKALACNTAVQDAISAFFRQSGEANVKAAASVAVSPPPRGQASYTGTESCA
                     DCHDDQVKFWKKTVHASAWQTLVDRGQQFDLDCIGCHVTAWDKPGGSNLGHNDPLRDV
                     QCETCHGPGSIHVA"
     gene            1574..1861
                     /gene="rpsP"
                     /locus_tag="VF516_18460"
     CDS             1574..1861
                     /gene="rpsP"
                     /locus_tag="VF516_18460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEX8109724.1"
                     /translation="MSVAIRFSRQGKKKSPFYRIVAADKRCARDGRFIELLGTYHPIT
                     KVLKLDKERFEKWRKVGAKPSGTLAAVIRRTERAAAAPASSGAPAPHKKTA"
     gene            1863..2213
                     /locus_tag="VF516_18465"
     CDS             1863..2213
                     /locus_tag="VF516_18465"
                     /inference="COORDINATES: protein motif:HMM:NF024483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEX8109725.1"
                     /translation="MAASNTPGPGSRPETRPDDREDREDDPRERRSDGPERAPDLAEL
                     IRFLAVNLVDRPDEVRVELVEERTANVYELEVAEADLGKVIGRGGKTARALRTVVQAV
                     APRSRKRTLVEILE"
     gene            2223..2726
                     /gene="rimM"
                     /locus_tag="VF516_18470"
     CDS             2223..2726
                     /gene="rimM"
                     /locus_tag="VF516_18470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEX8109726.1"
                     /translation="MRGDPRIEIGRISRVHGIRGEVVIATHDPESDLLDRLATLWIGG
                     VVRDVVRARSTPRGWLVQLAGVATRNDAEALRGQVVEVDRATLELDEDDVLLDDLIGC
                     RVQRVDGTPWGTIAAIEGGMQDRLVIHDGDVERQLPLVDEFVTDIDLEAGVVTVDPPE
                     GLPEHRR"
     gene            2742..>3387
                     /gene="trmD"
                     /locus_tag="VF516_18475"
     CDS             2742..>3387
                     /gene="trmD"
                     /locus_tag="VF516_18475"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEX8109727.1"
                     /translation="MRFTVVTILPELIEPALAAGVVGRAREAGAIAVDTVNPRDFTSD
                     RHRTVDDTPYGGGPGMVMKAEPLLAAIATAAPGETGGPRPHRIQMTPAGAPLTQARVR
                     ELAALPHIVLVCGRYEGIDERVIDLAIDEQLSIGDYVLSGGELAALVVIDAVARHVPG
                     VLGEASSVEDESLSAGLLEYPQYTRPLVLRAPGGAGELATPAILTSGNHAAIAAW"
BASE COUNT          452 a         1198 c         1289 g          448 t
ORIGIN      
        1 tggcgacgtg gatcgagccc ggcccgtggc aggtctcgca ctgcacgtcg cgcagcgggt
       61 cgttgtggcc gaggttcgat ccgccgggct tgtcccaggc ggtgacgtgg cagccgatgc
      121 agtcgaggtc gaactgctgg ccgcgatcga ccagggtctg ccacgcgctc gcgtgcacgg
      181 tcttcttcca gaacttgacc tgatcgtcgt ggcagtcggc gcacgactcg gtgccggtgt
      241 acgacgcctg gcccctgggc ggcgggctca ccgcgacgct cgcggcggcc ttgacgttgg
      301 cctcgccgct ctggcggaag aacgcgctga tcgcgtcctg gacggcggtg ttgcaggcca
      361 gcgccttgtg gatccggacc tggtcgagcg tgaagtagct gccgtgcggc gggacgacca
      421 gcgggtgggt cttgagccgg tcgcggtcgg cggcgagctg ggcgcgctcc ttctgcttct
      481 gggcgacgaa cgccgggtcg gcggatcggt cgctcgcgaa cgccttgagg tcggcgtcga
      541 gcgcggcgag ctggcggtcg atcacggcga gcttggcggc cgcggcgccg gggccgatcg
      601 cgtcggcgag cgggccgggg ccgcgcagcg tgacctcgaa ccgcgcgacg atctggccgc
      661 ggttggcggg cacgaccagc cagccgtcgc cgaccttctg cggctcgctc tcgatgcgct
      721 ccggctcggg cgccgcgagc cccaggccgg cgatcgcgag gtcgatcccg ccgatgtcgc
      781 gcaccagctt gacggcgtcc ttcttgctct ccgcctggac caggccgatc acgacctggg
      841 cgccctggcc gcgcagctgg gcgaccgcct gcttgcccgc ggcgaccgga tcgctcagag
      901 aaagcccggc gaccgcgccc ggcgcgatca ccccgaacac gccggccttg gccccgccga
      961 cgtcgatcag cttcgccggg ccggcgatcc cggtgggccc cccggccggc ggcgcattcg
     1021 ccaccacgcg tgccagccgc aggtggtcga ggctggcagc gccgttgggc aggtcggccg
     1081 gccccagccc cacggcgtcg gccaggagcg tcttctggta ggtccgggcc aggaggtcgg
     1141 ccttgagctc ctcctggacg gcgagctgcg gcggggtggg ggcatgggcg tagagcagcg
     1201 acccggcgtc gacgaacagc accgggccgg ccgcgcgggc ggagaccacc agctgcgcgg
     1261 tgcgcgacag gtcgcccagc gggtcggtgg tgcagccgca cgggccgatc tggccgcgga
     1321 cctcggcgac cgcgaacacc gtgaacgtcg gccgcgccgg cgcgggatcc ccggcggcgg
     1381 ccgtggattt cacctcggat ttcgagcagg aagcgccgag aagggccccg agagcggcgc
     1441 cgagagtggg gatgatcgcg ggcgctgcca gcagcccgac ccgacgcgac atggcgggtc
     1501 tataccatgg gttgacaggg ggtatgtggc tgggtagatt gccgcgctct caggaacacc
     1561 gctcgaggaa tccatgtccg ttgcgatccg cttttcacgt cagggcaaga agaagtcacc
     1621 gttctatcgg atcgtcgcag cggacaagcg ttgcgcgcgg gatggtcggt tcatcgagct
     1681 gctcgggacg tatcatccga tcaccaaggt cctcaagctc gacaaggagc ggttcgagaa
     1741 gtggcgcaag gtcggcgcca agcccagcgg tacgctggcg gcggtgatcc ggcgcaccga
     1801 gcgggcggca gctgccccgg cgtcatcggg ggccccggcc ccccataaga agaccgccta
     1861 gcatggcggc gtcgaacacc ccgggcccgg ggtcacgccc ggaaacccgc cccgacgatc
     1921 gcgaggatcg cgaggacgac ccccgcgagc gccggtccga tggccccgag cgcgcgccgg
     1981 acctggcgga gctgatccgc ttcctcgcgg tgaacctggt cgaccggccg gacgaggtcc
     2041 gggtcgagct cgtcgaggag cgcaccgcga acgtctacga gctcgaggtc gccgaggccg
     2101 atctcggcaa ggtcatcggc cgcggcggca agaccgcgcg ggcgctgcgc acggtggtcc
     2161 aggcggtggc gccgcggtcc cgcaagcgga cgctggtcga gatcctggaa tagcggcgct
     2221 ccgtgcgggg cgatccgcgg atcgagatcg gccggatctc gcgggtccat gggatccgcg
     2281 gcgaggtcgt gatcgccacc cacgacccgg agtccgacct gctcgaccgc ctggccacgc
     2341 tgtggatcgg cggggtcgtg cgcgacgtgg tccgggcgcg cagcacgccg cgcggctggc
     2401 tggtccagct cgccggcgtg gcgacccgca acgacgccga ggcgctgcgc ggccaggtcg
     2461 tcgaggtcga ccgcgcgacg ctcgagctcg acgaggacga cgtcctgctc gacgacctga
     2521 tcggctgccg ggtccagcgg gtcgacggca cgccgtgggg cacgatcgcc gcgatcgagg
     2581 gcgggatgca ggaccgcctg gtgatccacg acggcgacgt cgagcgccag ctgccgctgg
     2641 tcgacgagtt cgtcaccgac atcgacctcg aggccggggt ggtcacggtc gacccgcccg
     2701 agggcctgcc cgagcacagg cggtaggctg gggcgagcgc gatgcggttc accgtcgtca
     2761 cgatcctccc cgagctgatc gagcccgcgc tcgccgccgg cgtggtcggc cgcgcgcgcg
     2821 aggccggcgc gatcgcggtc gacaccgtca acccgcgcga cttcacgagc gaccgccacc
     2881 gcaccgtcga cgacacgccg tacggcggcg gcccgggcat ggtgatgaag gccgagccgc
     2941 tgctcgcggc gatcgcgacc gcggctcccg gcgagaccgg cgggccgcgc ccccatcgca
     3001 tccagatgac cccggcgggc gcgccgctga cccaggcccg cgtccgcgag ctcgccgcgc
     3061 tgccccacat cgtgctggtg tgcggccgct acgagggaat cgacgagcgc gtgatcgacc
     3121 tggcgatcga cgagcagctg tcgatcggcg actacgtgct gtcgggcggg gagctcgccg
     3181 cgctggtcgt gatcgacgcg gtcgcgcgcc acgtcccggg ggtgctcggc gaggcgagct
     3241 cggtcgagga cgagtcgctc agcgccgggc tgctcgagta cccgcagtac acccggccgc
     3301 ttgtgctgcg cgcgcccggc ggcgccggcg agctcgccac gccggcgatc ctgacctcgg
     3361 gcaaccacgc cgcgatcgcc gcgtggc
//
LOCUS       DASVOR010000952         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_301859,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000952 DASVOR010000000
VERSION     DASVOR010000952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301859"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VF516_18480"
     CDS             <1..565
                     /locus_tag="VF516_18480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109728.1"
                     /translation="TAHFGDGKLLLRSLRTSGARPRRYVLAVIADSTLNVAFLSVALR
                     VAATKLLTELESAAPSASSPVPTAIATGSTGSGRIPIVDQSRSVLEKTGLSWSGATTG
                     TGSGVGSSDVGVVDPASSTFLSACTKALAASIGPMAKVFVKEAVRKVCGERPFTRADG
                     PALLAHLAAGIEDSDDRASFQRATRTL"
     gene            646..>1509
                     /locus_tag="VF516_18485"
     CDS             646..>1509
                     /locus_tag="VF516_18485"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8109729.1"
                     /translation="MSLVLALITIPPMVAAAYLITASESARIERLTIESGKVAAMAGA
                     RMYGTALEAGIDAGIMTPRDVLEPSYEEIKGFDFGKNPRFHTRYDFFTDRTVVGFEDA
                     LLNSSPDFLYAVGGDINGYTPTHNSKFQRPLTGDAEKDLVGNRGKRKFWTDMHQAAAR
                     NLEPVLVQPYLRDTGESAWDVSSPIFVKGRHFGAFRVAVSRDSIANHKRSLLIQLVVV
                     FGFLTVITVGFIFFMLRRSMRPLEHLASLANEISTGEGLDRPIKPTSSDEIGMMAKSL
                     NRLRASLQAAMG"
BASE COUNT          234 a          534 c          516 g          225 t
ORIGIN      
        1 gacggcgcac ttcggcgacg gcaagctgct gctgcgcagc ctccgcacgt cgggcgccag
       61 gccgcggcgc tacgtgctcg cggtgatcgc ggactcgacg ctcaacgtcg cgttcctcag
      121 cgtcgcgctg cgggtcgccg cgaccaagct gctcaccgag ctcgagtccg cggcgccgtc
      181 ggcgagctcg ccggtgccca ccgcgatcgc gaccggcagc accggcagcg ggcggatccc
      241 gatcgtcgac cagtcgcgat cggtgctgga gaagaccggg ctgtcgtggt cgggcgccac
      301 cacgggcacc ggctcgggcg tcggcagctc ggacgtcggg gtcgtcgacc ccgcgagctc
      361 gacgttcctg tcggcgtgca ccaaggcgct cgccgcgagc atcggcccga tggccaaggt
      421 gttcgtgaag gaggcggtgc gcaaggtgtg cggcgagcgg ccattcaccc gcgccgacgg
      481 cccggcgctg ctcgcccacc tcgcggcggg gatcgaggac agcgacgacc gcgcgagctt
      541 ccagcgcgcg acccgcacgc tgtgacctgc acgccctgac atgtaacccc ctcacaggga
      601 gtgtcgacaa tgttcgagct catcagacgc agactgagcc tcaaggtgtc actcgtcctg
      661 gccctgatca cgattccgcc gatggtcgcg gcggcctacc tcatcacggc cagcgagtcg
      721 gcgcggatcg agcggctgac gatcgagtcc ggcaaggtcg cggcgatggc gggtgcgcgg
      781 atgtacggca cggcgctcga ggccgggatc gacgccggca tcatgacgcc ccgcgacgtg
      841 ctggagccgt cgtacgagga gatcaagggc ttcgacttcg gcaagaaccc gcggttccac
      901 acccggtacg acttcttcac cgaccgcacc gtggtcggct tcgaggacgc gctgctcaac
      961 tcgtcgcccg acttcctcta tgcggtgggc ggcgatatca acggctacac gccgacccac
     1021 aacagcaagt tccagcgccc gctgaccggc gatgccgaga aggacctcgt cggcaaccgc
     1081 ggcaagcgca agttctggac cgacatgcac caggccgcgg cgcgcaacct cgagccggtg
     1141 ctggtccagc cctacctgcg cgacaccggc gagtcggcgt gggacgtgtc gtcgccgatc
     1201 ttcgtcaagg gccgccactt cggcgcgttc cgcgtcgcgg tgtcgcgcga ctcgatcgcc
     1261 aaccacaagc ggtcgctgct gatccagctg gtcgtggtgt tcggtttcct gaccgtgatc
     1321 acggtcgggt tcatcttctt catgctgcgg cgctcgatgc ggccgctgga gcacctcgcc
     1381 tcgctggcca acgagatcag caccggcgag ggcctcgacc ggccgatcaa gccgacctcg
     1441 agcgacgaga tcggcatgat ggccaagtcg ctgaaccggc tgcgcgccag cctgcaggcc
     1501 gcgatggga
//
LOCUS       DASVOR010000953         1859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67778,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000953 DASVOR010000000
VERSION     DASVOR010000953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67778"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1601)
                     /locus_tag="VF516_18490"
     CDS             complement(<1..1601)
                     /locus_tag="VF516_18490"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX8109730.1"
                     /translation="MQASAPPSPRVAARTARTPRAWIPVVALLSCAPLAGCTEPAEDG
                     PAPVTSLDADGAPLAAVVSHWNALIARSGGTPAVRWGGRGTPLSLFGELSAPLGSIRA
                     DAAHRFLADNAALFQLSGDLAELDEQATVDSPLGSHVTFAQRLDGIRVFGAEVKVHFS
                     RDGRVVAVSNTSVPHVQLDTRPRISAQQALDLALAAVPAADRADSDAPELPELVIDAD
                     AGSPVLAYRAVVPTTGPTLEFFVDARTGARLGPPSDLNRYFVTGTGQVFKDNAVVATQ
                     NNNLVDSNDAASAVPSNAYSTVALQGLNGNNRLDGQFASGSASKSRAAGTSNSFLFLR
                     DNNAFSETMTYFHIDFAERYIQSLGFTNVNNRQQVFSVDRSKVDNSSYSPSTKNITYG
                     TGGVDDAEDGEVIIHEYGHSVQDNQVPGFGAVEEGGAMGEGFGDYFAATVNAALTGEF
                     QIVCIAEWDATFYAAGVPHCLRRLDSTKHYPGDKDGEVHDDGEMWSASLFQIRTALGA
                     ARADQLILQAHFLLSTRANFSDGSNA"
BASE COUNT          255 a          608 c          702 g          294 t
ORIGIN      
        1 gcgttgctgc cgtcgctgaa gttggcgcgg gtcgagagca ggaagtgggc ctggaggatc
       61 agctggtcgg cccgggcggc gcccaacgcc gtccggatct ggaacagcga ggccgaccac
      121 atctcgccgt cgtcgtggac ctcgccatcc ttgtcgccgg ggtagtgctt ggtggagtcg
      181 agccggcgca ggcagtgcgg cacgccggcg gcgtagaatg tcgcgtccca ctcggcgatg
      241 cagacgatct ggaactcgcc ggtcagggcg gcgttgacgg tggcggcgaa gtagtcgccg
      301 aagccctcgc ccatcgcccc gccctcctcg acggcgccga acccggggac ctggttgtcc
      361 tgcaccgagt ggccgtactc gtggatgatg acctcgccgt cttccgcgtc gtcgaccccg
      421 ccggtgccgt aggtgatgtt cttggtgctc ggcgagtacg acgagttgtc gaccttcgaa
      481 cggtcgaccg agaacacctg ctggcggttg ttgacgttgg tgaacccgag cgactggatg
      541 tagcgctcgg cgaagtcgat gtggaagtag gtcatggtct cgctgaacgc gttgttgtcg
      601 cgcaggaaga ggaagctgtt gctggtgccg gccgcgcgcg acttcgacgc cgagccggag
      661 gcgaactggc cgtcgagccg gttgttgccg ttcaggccct ggagcgcgac cgtggagtac
      721 gcgttgctcg gcaccgccga cgccgcatcg ttgctgtcga ccaggttgtt gttctgggtg
      781 gcgaccactg cgttgtcctt gaacacctgg ccggtgccgg tcacgaagta gcgattgaga
      841 tcgctcggcg ggccgagccg cgcgccggtg cgagcgtcga cgaagaactc gagcgtcggc
      901 ccggtggtcg gcacgaccgc gcgataggcc agcaccggcg acccggcgtc ggcgtcgatc
      961 acgagctcgg gcagctccgg tgcgtcactg tcggcgcgat cggcggcggg gaccgcggcg
     1021 agcgcgagat cgagggcctg ctgggccgag atccgcggcc gggtgtcgag ctggacgtgc
     1081 gggaccgagg tgttgctcac cgccacgacc cggccatcgc ggctgaagtg caccttgacc
     1141 tccgcgccga acacccggat accgtcgagc cgctgggcga acgtgacgtg gctgccgagc
     1201 gggctgtcga cggtggcctg ctcgtcgagc tcggcgaggt ctccggacag ctggaacagc
     1261 gcggcgttgt ccgcgaggaa gcggtgcgcg gcgtcggccc ggatcgagcc gagcggcgcg
     1321 gacagctcgc cgaacaggct cagcggcgtg ccgcgaccgc cccagcgcac cgccggggtc
     1381 ccgccgctgc gcgcgatcag cgcgttccag tgcgagacca cggccgcgag cggagcgccg
     1441 tccgcgtcga gcgaggtgac cggtgccggg ccgtcctcgg cgggttcggt gcaaccggcg
     1501 agcggcgcgc acgacagcag cgcgacgacg gggatccacg cccgcggtgt acgggccgtg
     1561 cgagccgcaa cacgcggcga tggcggagcg cttgcttgca tgatcgagat tcctccctcg
     1621 cgtcgggatg tgaacgtcga ccctagccgc gtggctcacc cgtgaggtgg cccgcggctg
     1681 ccgcaaagcg aggcgccgcc ggctacagct tcgggccgcc ggcgcggacc tcggcggacg
     1741 cctgcgcctc gtacttcttg atgttgtcgc ggaacagcga ggcgaccttc ttggccttct
     1801 cgtcgtaggc ggccttgtcg gcccaggtgt tgcgcggctg caggatctcg gccggcacg
//
LOCUS       DASVOR010000954         2711 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_67806,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000954 DASVOR010000000
VERSION     DASVOR010000954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67806"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..>2711
                     /locus_tag="VF516_18495"
     CDS             223..>2711
                     /locus_tag="VF516_18495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MXAN_5808 family serine peptidase"
                     /protein_id="HEX8109731.1"
                     /translation="MRSRLNLALVGAAGVSALVLTMIRQSPEGVLSIELNTQEVRAAP
                     GETSAPRHDLSALRIFNMTLLRIKERYVDPARVDPKKMLYAALDSVQFNIPEVLVEPD
                     PVHNRVRVTVNDKPETFETDDVDSPWRLAGKLKKVFRFIETNMNAGNSGADLAKVEYA
                     AVNGMLSTLDPHSILMDPEQARDMEVSTSGKFGGLGIVIRMIEKRLTVIKPMKDTPAW
                     RKGIKAGDHIVRINQEPTENLTSNEAVDRMRGDPRTGVTLYVERKGVDGLMKFDLIRD
                     LIKIVGVEHKLLSAGETGSPDRSGGAADKTARGTDKRVGYIRVRQFSKGIAADVAEAM
                     REMSAKGAASWILDLRGNPGGLLEEAVQLADLFVDNGTIVTTVSGRDREARRAERGFG
                     DTTSPLAVLVSGNSASASEIVDGALKNLDRAVTIGTRTFGKGSVQELYDNDDHSKLKL
                     TIAQYLTPGDRSIQNLGIVPDIQLQRMYVPDKNDSPQDFVRLLPPTRTYGEKDLDAHL
                     VSTYARDIDKPAYELPYLVERKKPATGTPAEVKPPDEDDATDDDEIVEDFEIRFAKQL
                     VSSVSAPTRPRLIAGAGKIVQGVRAEEEKKLVAALQTLGVDWTAAPATAAGKPSLEVS
                     VAASPPGQVKAGDVVTVTATVKNTGTDAAYRVLSRIQGDDPVFEDTELPIGKIAPGET
                     RTYSTRLQVPKDALDRVDRLGLEVREAHNALAHITPTELRIQAAPRPVFAYAWQLIDD
                     GNGDGLVQRGEHYRLQVQIKNTGAGPTQEAAVLLRNATGDGVVLDKSRAELKAPLAPG
                     EVKQIEFPLATDATLRGDEMVVELIAYDAAL"
BASE COUNT          475 a          964 c          913 g          359 t
ORIGIN      
        1 accgacatcg atgaccttgc ccgccggctt gcggcggcgc tttgccccac ccttggggat
       61 cgtcgagccg gaggcagcgg cctcggaccc ggctccggag cctcgtgact ttcgctcggc
      121 catcgctgcc cgagcaccat actacacggc attactcgac ggaggcttcc ggccgcgcag
      181 atcgttcgtg gcgcgtcgag ctttgcatcc tataatcgcc ggatgcgcag ccggttgaac
      241 ctggccctgg tgggggccgc aggtgtgtcc gccctcgtgc tcaccatgat ccggcagtcg
      301 ccggaagggg tcctctccat cgagctcaac acccaggagg tccgagcggc ccccggggag
      361 acttccgccc ctcgccacga cctgtcggcc ctcaggatct tcaacatgac cttgctccgg
      421 atcaaggagc gctacgtcga cccggcgcgg gtcgatccca agaagatgct gtacgcggcg
      481 ctcgacagcg tgcagttcaa catccccgag gtcctggtcg agcccgatcc ggtgcacaac
      541 cgggtgcggg tcaccgtcaa cgacaagccc gagacgttcg agaccgacga cgtcgactcg
      601 ccgtggcggc tcgccggcaa gctcaagaag gtgttccgct tcatcgagac gaatatgaac
      661 gccggcaaca gcggcgccga cctcgccaag gtcgagtacg cggcggtcaa cggcatgctg
      721 tcgacgctcg atccgcactc gatcctgatg gatcccgagc aggcgcgcga catggaggtg
      781 tcgaccagcg gcaagttcgg cgggcttggc atcgtgatcc ggatgatcga gaagcggctc
      841 accgtgatca agccgatgaa ggacacgccg gcgtggcgca agggcatcaa ggccggcgac
      901 cacatcgtgc ggatcaacca ggagccgacc gagaacctga cctcgaacga ggcggtcgat
      961 cgcatgcgcg gcgacccgcg gaccggcgtg acgctgtacg tcgagcgcaa gggcgtcgac
     1021 ggcctgatga agttcgatct gatccgcgac ctgatcaaga tcgtcggggt cgagcacaag
     1081 ctgctctcgg ccggcgagac cggaagcccc gaccgctccg gtggggcggc ggacaagacc
     1141 gcccgtggga ccgacaagcg cgtcggctac atccgggtca gacagttctc caaggggatc
     1201 gcggccgacg tcgccgaggc gatgcgcgag atgagcgcca agggagcggc ctcgtggatc
     1261 ctcgacctgc gcggcaaccc cggcggcctg ctcgaggagg cggtccagct cgccgatctg
     1321 ttcgtcgaca acggcacgat cgtcaccacc gtgtcgggcc gcgaccgcga ggcgcgccgc
     1381 gccgagcgcg ggttcggcga caccacgtcg ccgctcgcgg tgctggtcag cggcaactcg
     1441 gcgtcggcct ccgagatcgt cgacggcgcg ctcaagaacc tcgaccgcgc cgtgaccatc
     1501 ggtacccgga cgttcggcaa gggctcggtc caggagctct acgacaacga cgaccactcc
     1561 aagctcaagc tgacgatcgc ccagtacctg acgcccggcg atcgctcgat ccagaacctc
     1621 ggcatcgtcc ccgacatcca gctccagcgc atgtacgtgc cggacaagaa cgactcgccg
     1681 caggacttcg tgcgcctctt gccgccgacc cggacctacg gcgagaagga cctcgacgcc
     1741 cacctggtgt cgacctacgc gcgcgacatc gacaagccgg cctacgagct gccgtacctg
     1801 gtcgagcgca agaagccggc caccggcacg ccggccgagg tcaagccgcc cgacgaggac
     1861 gacgccacgg acgacgacga gatcgtcgag gacttcgaga tccggttcgc caagcagctg
     1921 gtgtcgagcg tgtcggcgcc gacccggccg cggctgatcg ccggcgccgg caagatcgtc
     1981 cagggcgtgc gcgccgagga ggagaagaag ctggtcgccg cgctccagac gctcggcgtc
     2041 gactggaccg ccgcgccggc gaccgccgcc ggcaagccca gcctcgaggt ctcggtcgcc
     2101 gcgtctccgc cgggccaggt caaggcgggc gacgtcgtca ccgtgaccgc cacggtcaag
     2161 aacaccggca ccgacgccgc gtaccgcgtg ctgtcgcgga tccaggggga cgacccggtg
     2221 ttcgaggaca ccgagctgcc gatcggcaag atcgcgccgg gcgagacccg gacctactcg
     2281 acccggctcc aggtgcccaa ggacgcgctc gatcgggtcg accggctcgg gctcgaggtc
     2341 cgcgaggccc acaacgcgct cgcgcacatc acgccgaccg agctccggat ccaggccgcg
     2401 ccgcgcccgg tgttcgcgta cgcctggcag ctgatcgacg atggcaacgg cgacggcctg
     2461 gtccagcgcg gcgagcacta ccggctgcag gtccagatca agaacaccgg cgccgggccg
     2521 acccaggaag ccgcggtgct gctccgcaac gcgaccggcg acggcgtcgt gctcgacaag
     2581 tcgcgcgccg agctcaaggc cccgctggcg cccggcgagg tcaagcagat cgagttcccg
     2641 ctcgcgaccg acgcgacgct ccggggcgac gagatggtgg tcgagctgat cgcctacgac
     2701 gcggcgctcg a
//
LOCUS       DASVOR010000955         5676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_10091,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000955 DASVOR010000000
VERSION     DASVOR010000955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5676
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10091"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="VF516_18500"
     CDS             complement(<1..1040)
                     /locus_tag="VF516_18500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109732.1"
                     /translation="MPEPRPQTVSELSLEAAQALASRPPVAPVVDRKRALIYAALLGL
                     AVMWLTGAIPRWGQWYSEQPIYRAQAHALLAGRFALGHDPAGLGLDLAWIDGGVQQVW
                     GLGVPLWLALWEAIGRVIQLTPFPDRLAMLFGVVAMMYVLVRAWLGPGGERSYASRGA
                     LLFTALLPGVLTMLRGRVAVYEEAEAYAYGAAMVLLGGVIMMVRRPSTARYVLLLAFA
                     GATGLIRPTVWFYGAAAAVVATAIYLRHRVRHRGALRGSLGAVALAWGLFLAGGAVLY
                     ASNAARFGAGGEFGHRVNLGALYVTRFTNPYDAVPAWTAARELAGGMFTRPELSTHRG
                     FYDAGIHPGQARA"
     gene            1263..1754
                     /locus_tag="VF516_18505"
     CDS             1263..1754
                     /locus_tag="VF516_18505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109733.1"
                     /translation="MRSIALSTALLMAVPVAAYADPPQTQYDRDRAYDHDHDRDRDRD
                     RAYFDHERYDRWNDSHWARDYHGRWRSFGQVFNARNDRQFINLGGGRYHMLRLEAVRG
                     EPKIDRVTILFNDGTSQTVQLDSELTGGAGEVIRLDPDRRVRRVIVFTEPNTRGLYSV
                     HAG"
     gene            1810..2784
                     /locus_tag="VF516_18510"
     CDS             1810..2784
                     /locus_tag="VF516_18510"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8109734.1"
                     /translation="MSEPEIAVLVPCFNESAAIAKVVADFRAALPRATVYVYDNNSTD
                     DTSAVAAAAGAEVRRETRRGKGNVVRRMFQDIEADVYVMVDGDDTYDAAVAPSLVDRL
                     VADNLDMVVGKRVETHQAAYRAGHRFGNRLLTGLVRWLFHAGIDDMLSGYRVFSRRFV
                     KSFPSFSREFEIETELTVHAMQMRMPIAELPTDYKERPPGGASKLRTFRDGWRILLTI
                     TNLMRNERPLMFFSLIGLAIAILAIALGIPVVLAFLDTGEVRRFPTAILCSGLGVIAV
                     VCIATGLILDLVAHVRREAKRLVYLQHPAPRARVTRPDRDTAIPRQAG"
     gene            2910..4160
                     /locus_tag="VF516_18515"
     CDS             2910..4160
                     /locus_tag="VF516_18515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEX8109735.1"
                     /translation="MDVELMRRLDRVLGDAACSALATAHRLRKPFTQARRPIRKIAVM
                     KFFGMGSIIVASRSLAALRDAYPGAEIHFVTFAGNRGILDILDMTDHDHYVDPSTPQA
                     FVKTTLAVARALRRARCDLVLDLEFFAKFPLVLGSLAGIPQKAGFYLTSENWRRELLD
                     FTGFYNGYFHTSDIFLSLVYLCATGDHYYLGFNDWAAKYRYPRIEPGELERAQLRARL
                     AGHGIGRGDPLFVINPNTSPDLAPEARKWPRERYAQLADELAAHTPGARVCFIGAPNE
                     RAYVHGIAELCRTPRRHVLAGELSLRELLVLFAESKLVVSNDSGPMHMTCLVDAPVVG
                     LFFADTPTLFAPLGSRVRCVAPPLYSIPLFSVYNGKDVAVGKPSSEVGNAAACAVSLD
                     TVMTEVRELLAQRPVLRSSDAGGT"
     gene            4157..5329
                     /locus_tag="VF516_18520"
     CDS             4157..5329
                     /locus_tag="VF516_18520"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX8109736.1"
                     /translation="MTRAPSLERLVRATLLDGARVRIDLDAAAIVYACELMRGAVLAG
                     KKVLLCGNGGSAADAQHIAAELVGRFVIERRPLAAIALTTDTSALTAIANDYGFEHVF
                     SRQVEALGAEGDVLIAITTSGTSNNVLAAVAAARARGMKVIGLTGGKGAAFAAACDAG
                     VAVPSTVVARIQECHITIGHLLCEAVDLAFAGAAEQLALPVAAPAVTPGDVGALLDHA
                     LLGEPGVAKLRTLDQMITWREGQRARKRTVVWTNGVFDVLHVGHLASLRGARQFGDVL
                     IVGVNGDASVRANKGPERPIYRCEERVALLAALELVDAILVFDDPTPERVLAQVRPDV
                     HVKGADYAPPDGKPVPERAVVEAYGGRIEFLPLVPGRSSTDTITRLGTHLGSRLRS"
     gene            5326..>5676
                     /locus_tag="VF516_18525"
     CDS             5326..>5676
                     /locus_tag="VF516_18525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HEX8109737.1"
                     /translation="MTGPRRALFVDRDGTLIVDVGYPRDPAQVEPLPGALDALRALQA
                     HFALVIVSNQSGIGRGLITQAEARAVHARVVERFGQAGVAFAGAYYCPHDPGAACVCR
                     KPAPGLLLDAARELG"
BASE COUNT          803 a         2137 c         1957 g          779 t
ORIGIN      
        1 gcacgcgcct gcccggggtg gatgccggcg tcgtagaacc cgcggtgggt gctgagctcg
       61 gggcgggtga acatgccgcc cgccagctcc cgggcggcgg tccacgccgg caccgcatcg
      121 taggggttgg tgaaccgggt cacgtacagc gcgccgaggt tgacccggtg gccgaactcg
      181 ccaccggcgc cgaaccgcgc cgcgttgctc gcgtacagca ccgcgccgcc ggcgaggaac
      241 aggccccagg cgagcgccac cgcgccgagc gatccgcgca gcgcgccgcg gtggcggacc
      301 cggtggcgaa gatagatcgc ggtcgcgacc accgccgccg cggcgccgta gaaccacacc
      361 gtcgggcgga tcaggccggt cgcgccggcg aacgcgagca gcagcacgta gcgcgcggtc
      421 gacggccgcc gcaccatcat gatcacgccg ccgagcagca ccatcgcggc gccgtaggcg
      481 taggcctcgg cctcctcgta gaccgcgacc cgcccgcgca gcatggtcag cacgccgggc
      541 aacagcgcgg tgaacagcag cgcgccgcgc gaggcgtacg agcgctcgcc gcccggcccg
      601 agccacgccc ggaccagcac gtacatcatc gcgacgacgc cgaacagcat cgccaggcga
      661 tcggggaacg gcgtcagctg gatcacccgg ccgatcgcct cccacagcgc gagccacagc
      721 ggcacgccga ggccccacac ctgctgcacg ccgccgtcga tccaggccag gtcgagcccc
      781 aggcccgccg gatcgtggcc gagcgcgaac cggccggcga gcagcgcgtg ggcctgcgcg
      841 cggtagatcg gctgctcgct gtaccactgg ccccagcgcg ggatcgcgcc ggtcagccac
      901 atcaccgcca ggccgagcag cgcggcgtag atcagcgcgc gcttccggtc gacgactggc
      961 gccaccggcg gccgcgatgc cagggcctgc gccgcctcca gtgacagctc ggacaccgtc
     1021 tgcggccggg gctcaggcat gcggttcgcc ggggctcgag gccacgcgca ctcgtagcac
     1081 gaagcggcgc ggaccgtcca atcggcctag gaaccgctcg catcggggtt tttgccgggc
     1141 gcgctgtccc gcatgccggg cggctgcgcg cgtccgtgcg ccgccgtctt ctgcaatttc
     1201 acggctcagc gatcggcccg cgcgatgcaa tcaccgacgg taaaccccac aaggagctag
     1261 cgatgagatc gattgccctg tcaactgcac tgctcatggc agtcccggtg gcggcgtatg
     1321 cagatccgcc acagacgcag tacgaccgcg accgcgccta tgatcacgac cacgaccgcg
     1381 accgcgaccg cgaccgcgcc tacttcgacc acgagcgcta cgaccgctgg aacgacagcc
     1441 actgggcgcg cgactaccat ggacgctggc gctcgttcgg ccaggtcttc aacgcgcgca
     1501 acgaccgtca gttcatcaac ctcggcggcg gccgttacca catgctgcgg ctcgaggccg
     1561 tgcgcggtga gccgaagatc gaccgggtga cgatcctgtt caacgacggg accagccaga
     1621 ccgtccagct cgacagcgag ctgaccggcg gcgccggcga ggtgatccgg ctcgaccccg
     1681 atcgccgggt ccgccgcgtg atcgtgttca ccgagcccaa cacgcgcggg ctgtactcgg
     1741 tccacgccgg gtaacgccag gccggcggac cgcgaggcga gccgggaggc gaggtggtaa
     1801 gaaaacctcg tgtccgagcc cgagatcgcg gtcctcgtcc catgcttcaa cgaatccgcg
     1861 gcgatcgcga aggtcgtcgc ggattttcgc gccgcgctgc cgcgcgccac ggtgtacgtc
     1921 tacgacaaca actcgaccga cgacacctcg gcggtcgcgg cggccgcggg cgccgaggtc
     1981 cgccgggaga cccggcgcgg caagggcaac gtggtgcgcc ggatgttcca ggacatcgag
     2041 gccgacgtct acgtcatggt cgacggcgac gacacctacg acgccgcggt ggcgccgagc
     2101 ctggtcgacc ggctggtcgc cgacaacctc gacatggtcg tcggcaagcg ggtcgagacc
     2161 caccaggccg cctaccgcgc cggccaccgg ttcggcaacc ggctgctgac cggcctggtg
     2221 cgctggctgt tccacgccgg gatcgacgac atgctgtcgg gctaccgggt gttctcgcgc
     2281 cggttcgtca agagcttccc gtcgttctcc cgcgagttcg agatcgagac cgagctcacc
     2341 gtccacgcga tgcagatgcg gatgccgatc gccgagctcc ccaccgacta caaggagcgc
     2401 ccgccgggcg gggccagcaa gctgcgcacg ttccgcgacg gctggcggat cctgctcacg
     2461 atcaccaacc tgatgcgcaa cgagcggccg ctgatgttct tctcgctgat cggcctggcg
     2521 atcgcgatcc tcgcgatcgc gctcggcatc ccggtcgtgc tcgcgttcct cgacaccggc
     2581 gaggtccgcc ggttcccgac cgcgatcctg tgcagcggcc tgggcgtgat cgcggtggtg
     2641 tgcatcgcga ccggcctgat cctcgatctg gtcgcccacg tgcgccgcga ggccaagcgg
     2701 ctggtctacc tccagcaccc cgcaccgcgc gcccgcgtca cccggccgga ccgcgacacc
     2761 gcgatcccgc gccaggccgg ctgaaccgcg gcagccgtgt cgcggtcggt ccgcgccggc
     2821 cggcaactcg cggtgaccgc gtgatttccg cccgcgatac gaggtggatt gcttgaccgt
     2881 gcttccgtcc gcatatagac ctgagcgtca tggacgtcga gctgatgcgc cgtctcgacc
     2941 gggtgctggg ggacgccgcc tgcagcgcgc tcgccacggc gcaccggctg cgcaaaccat
     3001 tcacgcaggc gcgccgcccg atccggaaga tcgccgtgat gaagttcttc ggcatgggct
     3061 cgatcatcgt cgcgagccgc agcctggcgg cgctgcgcga cgcctacccc ggcgccgaga
     3121 tccacttcgt cacgttcgcc ggcaaccgcg gcatcctcga catcctcgac atgacggatc
     3181 acgaccacta cgtcgatcca tcgaccccgc aggcgttcgt caagacgacg ctggcggtgg
     3241 cgcgagcgct gcgccgcgcc cgctgcgacc tggtgctcga tctggagttc ttcgccaagt
     3301 tcccgctcgt gctcggcagc ctggccggca tcccgcagaa ggccgggttc tacctgacgt
     3361 cggagaactg gcggcgcgag ctgctcgact tcaccgggtt ctacaacggg tactttcaca
     3421 ccagcgacat cttcctgtcg ctggtctacc tgtgcgcgac cggcgatcac tactacctcg
     3481 gcttcaacga ctgggccgcg aagtaccgct atccgcggat cgagcccggc gagctcgagc
     3541 gcgcgcagct gcgcgccagg ctcgccggcc acggcatcgg ccgcggcgat ccgctgttcg
     3601 tgatcaaccc gaacacgtcg cccgatctcg cgcccgaggc gcgcaagtgg cccagggagc
     3661 gctacgcgca gctcgccgac gagctcgcgg cccatacccc gggcgcccgg gtgtgcttca
     3721 tcggcgcgcc caacgagcgc gcctacgtcc acggcatcgc cgagctgtgc aggacgccgc
     3781 gccgccacgt gctcgccggc gagctgtcgc tgcgcgagct gctggtgctg ttcgccgagt
     3841 ccaagctcgt ggtgtccaac gactccgggc cgatgcacat gacgtgtctg gtcgacgcgc
     3901 cggtcgtcgg cctgttcttc gccgacacgc cgacgctgtt cgcgccgctc ggctcgcggg
     3961 tgcgctgcgt ggcgccgccg ctgtactcga tcccgttgtt cagcgtgtac aacggcaagg
     4021 acgtcgcggt cggcaagccg tcgagcgagg tcggcaacgc cgcggcgtgc gcggtcagcc
     4081 tggacaccgt gatgaccgag gtccgcgagc tgctcgcgca gcggccggtg ctgcggtcgt
     4141 ccgacgccgg cgggacatga cgcgcgcccc ctcgctcgag cgcctggtcc gcgcgacgct
     4201 gctcgacggc gcccgcgtcc ggatcgatct cgacgccgcg gcgatcgtgt acgcctgcga
     4261 gctgatgcgc ggcgcggtgc tcgccggcaa gaaggtcctg ttgtgcggca acggcggcag
     4321 cgcggccgac gcccagcaca tcgcggccga gctggtcggg cggttcgtga tcgagcgccg
     4381 gccgctcgcg gcgatcgcgc tcaccaccga cacctcggcg ctgaccgcga tcgccaacga
     4441 ctacggcttc gagcacgtgt tctctcgcca ggtcgaggcg ctcggcgccg agggcgacgt
     4501 gctgatcgcg atcaccacca gcgggacgtc gaacaacgtg ctcgccgccg tcgccgccgc
     4561 ccgcgcgcgc ggcatgaagg tgatcgggct gaccggcggc aagggcgctg cgttcgccgc
     4621 cgcgtgcgac gccggggtcg cggtgccgtc gacggtggtc gcccggatcc aggagtgcca
     4681 catcacgatc ggccacctgc tgtgcgaggc ggtcgacctg gcgttcgcgg gcgcggccga
     4741 gcagctcgcg ctgccggtcg ccgcgccggc ggtgaccccg ggcgacgtcg gggcactgct
     4801 cgaccatgcg ctgctcggcg agcccggcgt cgccaagctg cgtacgctgg atcagatgat
     4861 cacctggcgc gagggtcagc gggcgcgcaa gcgcaccgtg gtgtggacca acggcgtgtt
     4921 cgacgtcctc cacgtcggcc acctcgcgtc gctgcgcggc gcccgccagt tcggcgacgt
     4981 cctgatcgtc ggggtcaacg gcgacgcctc ggtgcgcgcc aacaagggcc cggagcggcc
     5041 gatctatcgc tgcgaggagc gcgtcgcgct gctcgcggcg ctcgagctgg tcgacgcgat
     5101 cctcgtgttc gacgacccga ccccggagcg cgtgctcgcc caggtccggc ccgacgttca
     5161 cgtcaagggc gccgactacg cgccgcccga cggcaagccg gtccccgagc gcgccgtcgt
     5221 cgaggcctac ggcggccgga tcgagttcct gccgctggtc ccgggccggt cgtcgaccga
     5281 cacgatcacc cgcctgggca cccacctggg cagccgcctg cggtcatgac cgggccgcgc
     5341 cgcgcgctgt tcgtcgaccg cgacggcacg ctgatcgtcg acgtcggcta cccgcgcgac
     5401 cccgcgcagg tcgagccgct gcccggcgcg ctcgacgccc tgcgcgcgct gcaggcccac
     5461 ttcgcgctcg tgatcgtgtc caaccagtcc ggcatcggcc gcggcctgat cacgcaggcc
     5521 gaggcccgcg cggtccacgc ccgcgtcgtc gagcggttcg gtcaggccgg cgtcgcgttc
     5581 gccggcgcct actactgccc gcacgacccg ggcgccgcct gcgtctgccg caagcccgcc
     5641 ccgggcctct tgctcgacgc cgcccgcgag ctcggc
//
LOCUS       DASVOR010000956         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_418108,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000956 DASVOR010000000
VERSION     DASVOR010000956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418108"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..329)
                     /locus_tag="VF516_18530"
     CDS             complement(<1..329)
                     /locus_tag="VF516_18530"
                     /inference="COORDINATES: protein motif:HMM:NF017568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase domain-containing protein"
                     /protein_id="HEX8109738.1"
                     /translation="MVGTATDVDPQLGMLLGDTTTEIDVARKNRVFCNRNLRMDTIEM
                     IGFDMDYTLALYHQDRLEQLSMELTLQKLVDKHGYAPDILQLSYDPRWAIRGLVVDKK
                     YGNVFKA"
     gene            368..931
                     /gene="hpt"
                     /locus_tag="VF516_18535"
     CDS             368..931
                     /gene="hpt"
                     /locus_tag="VF516_18535"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEX8109739.1"
                     /translation="MVVPPAWNRPSEPFAELISAAAIAARVAELGAQITADYAPLSRA
                     VDVVVVGVLKGSVIFLSDLVRQIAAPVYLEFIGISSYGDATVSSGVVQITQDLSRPIE
                     GKHVIVVEDIVDTGHTVHYLLENLATRRPASIKLCALLHKPDRAEREVKIDYLGFTIP
                     NKFVVGYGLDVAQQFRNLPFIGYVQGP"
     gene            928..1926
                     /locus_tag="VF516_18540"
     CDS             928..1926
                     /locus_tag="VF516_18540"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX8109740.1"
                     /translation="MNAEGLHKLPAALFEIGKLIGSELDPGILLSRIAELICQLLGAK
                     ACSVMLLDADRKRLLARAAYGVRTERMQSLSFRIGEGVAGWVVERGEPALIADAAADP
                     RFVVLPGNQTPIASMLCVPLIARGQRVGVVTATSERTGAFDADHLELVRFIARTIALD
                     IENVRLHRVAVTDPLTGAFNREFLVQRLPQEIAAARDHDRPLSLALVDVDRFKAVNDR
                     YGHHVGDAVLTEVARRLRGAIRAGDLLVRYGGEEFLAVLPRTDAGRAWEVGERMRQRV
                     SERAFDLGDGIALLLRVSVGVAQWRSGELMPGLIERADTVLYGAKDRGRNRVEVAP"
BASE COUNT          282 a          699 c          706 g          328 t
ORIGIN      
        1 tccgccttga acacgttgcc gtacttcttg tcgacgacca ggccgcggat cgcccaccgc
       61 gggtcatacg acagctgcag gatgtccggc gcgtagccgt gcttgtcgac cagcttctgc
      121 agcgtgagct ccatcgacag ctgctcgagc cggtcctggt ggtacagcgc cagcgtgtag
      181 tccatgtcga acccgatcat ctcgatcgtg tccatgcgca ggttgcggtt gcagaacacg
      241 cggttcttgc gggccacgtc gatctcggtc gtggtgtccc ccaagagcat gccgagctgc
      301 ggatcgacgt ccgtggccgt tccgaccatt ccggccaatt gtagcctgct tgggatacat
      361 tcgggacatg gtcgtgcccc ccgcgtggaa ccgtccgtcc gagccgttcg ccgagctgat
      421 ctccgcggcg gccatcgccg cccgggtcgc cgagctcggg gcccagatca cggcggacta
      481 tgcgccgctg tcacgcgccg tcgacgtcgt ggtggtcggc gtgctcaagg gctcggtgat
      541 cttcctgtcc gacctggtgc gacagatcgc ggccccggtg tacctggaat tcatcggtat
      601 ctcgtcgtat ggcgacgcga ccgtgtcgtc cggcgtggtc cagatcaccc aggacctgtc
      661 gcggccgatc gagggcaagc acgtgatcgt cgtcgaggac atcgtcgaca ccggtcacac
      721 cgtgcactac ctgctcgaga acctggcgac ccgccggccg gccagcatca agctgtgcgc
      781 gctgctccac aagccggacc gggccgagcg agaagtcaag atcgattacc tgggcttcac
      841 catccccaac aagttcgtcg tcggctacgg cctggatgtc gcacagcagt tccggaacct
      901 accgttcatc ggctacgtac aggggccatg aacgccgaag ggctgcacaa gctccccgct
      961 gcgctgttcg agatcggcaa gctgatcggg tcggagctcg accccggcat cctgctgtcg
     1021 cggatcgccg agctgatctg ccagctgctc ggggccaagg cgtgctcggt gatgctgctc
     1081 gacgccgacc gcaagcggct gctcgccagg gcggcctacg gcgtgcggac cgagcgcatg
     1141 cagtcgctgt cgttccggat cggcgagggc gtcgccggct gggtggtcga gcgcggcgag
     1201 ccggcgctga tcgccgacgc cgccgccgat ccgcggttcg tcgtgctgcc cggcaaccag
     1261 acgccgatcg cgtcgatgct gtgcgtgccc ctgatcgcgc gcggccagcg cgtcggggtg
     1321 gtcaccgcga ccagcgagcg gaccggcgcg ttcgacgccg atcacctgga gctggtccgg
     1381 ttcatcgccc ggaccatcgc gctcgacatc gagaacgtgc ggctgcaccg ggtcgcggtg
     1441 accgacccgc tgaccggcgc gttcaaccgc gagttcctgg tccagcggct gccgcaggag
     1501 atcgccgccg cgcgcgatca cgaccggccg ctgtcgctcg cgctggtcga cgtcgaccgc
     1561 ttcaaggcgg tcaacgaccg ctacggccac cacgtcggcg acgccgtgct caccgaggtc
     1621 gcccgccggc tgcgcggcgc gatccgcgcc ggcgatctgc tggtccgcta cggcggtgag
     1681 gagttcctcg cggtgctgcc ccggacggac gcggggcggg cctgggaggt cggcgagcgg
     1741 atgcggcagc gggtgtccga gcgcgcgttc gatctcggcg atggcatcgc gctgctgctg
     1801 cgggtgtcgg tcggcgtggc gcagtggcga tccggcgagc tgatgcccgg gctgatcgag
     1861 cgcgccgaca cggtgctgta cggcgccaag gatcgcggcc gcaaccgcgt cgaggtcgcg
     1921 ccgtgaccga gagcggcgac ggcggcggcg gcaagccacc ggatcgcccg gcgcgccccg
     1981 accccgccga cgaggcgatg taccggtcgg cgtcg
//
LOCUS       DASVOR010000957         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447719,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000957 DASVOR010000000
VERSION     DASVOR010000957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447719"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..683
                     /locus_tag="VF516_18545"
     CDS             162..683
                     /locus_tag="VF516_18545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEX8109741.1"
                     /translation="MAKPTRSNPSRTTTGLDLRSLLHVPQHDLQAQLAIRLERLGFPA
                     LRPALVQLLSVIGSGIAVSDAGVRLSDLVSQVSLPKQTVGDLLDELESMRMVERFPDP
                     DHGVIKRVRLGPKGKVWAAEVKRVSAATEAKWATRIGSKKMKSLRALLEELAATVEDR
                     GTGRANGTRQHKR"
     gene            complement(707..1588)
                     /locus_tag="VF516_18550"
     CDS             complement(707..1588)
                     /locus_tag="VF516_18550"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8109742.1"
                     /translation="MCPVCRQRPAEVGVLCEECRDELSSPIKIMPEQIQLHGAQPTMA
                     ALIDLWGRPHRLDPRTLIGRQVDGQGLAILEPSVSRHHAHVILDNDEWTVRDLGSANG
                     TFLDDKLISTPVAMHEGDRVRFGHIAFYIVEDVARLPPPRVGRILTATIKPVERRAVR
                     TAPTFPIEPLAEEDHTDVGLPVMSFKMHEPTGGGGGLIEVDGKQVQLTTTQFELMALM
                     IKRMAGEHHQPELVRGFVRSSELIADLSWDTREPSENHVKQLVRRVRRALIKSEIGDL
                     IESRHRFGYRLRAIPRE"
     gene            complement(1836..>2292)
                     /locus_tag="VF516_18555"
     CDS             complement(1836..>2292)
                     /locus_tag="VF516_18555"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8109743.1"
                     /translation="VLQGFHGEFSHRGANEYAVDFNCPVATPVTAARPGVVVAINAGA
                     QGSGTTPEFLDDRRANFVLVQHSDGTLGEYMHLAPSGVEVTPGQAVRRGQELGLSGNT
                     GFSSTPHLHFQVMTAADDGIAKRSFPFELAVAPNRASPPVLGQRYAAWE"
BASE COUNT          340 a          783 c          820 g          349 t
ORIGIN      
        1 ggggcttggc cgaagggatt tcacaggata aaacggtgat gcgccctacc gccgttgcgc
       61 tccggagcgt ggcacccgcg tgactttgcg acgaattcgg gttccgatcc tggggcagct
      121 ttggtatgac gcgaatccct cagggcgccc gtttcgtgac catggcgaag ccgacccgtt
      181 cgaacccttc caggaccacc acgggccttg acctcaggtc tctgctccac gtcccgcaac
      241 acgatctgca ggcgcagctc gcgatccggc tggagcgcct cgggtttccc gcgctgcgcc
      301 cggcgctggt ccagctgctc tcggtgatcg gctcgggcat cgcggtgtcc gacgccggtg
      361 tccggctttc ggacctcgtg tcccaggtgt cgctgcccaa gcagacagtc ggcgacctgc
      421 tggacgagct cgagagcatg cggatggtcg agcggttccc cgaccccgat cacggggtga
      481 tcaagcgcgt ccgcctgggc cccaagggca aggtgtgggc cgccgaggtc aagcgggtgt
      541 ctgcggcgac cgaggccaag tgggcgaccc ggatcggcag caagaagatg aagtcgctgc
      601 gcgcgctgct cgaagagctc gcggcgaccg tcgaggaccg cggtaccggc cgcgccaacg
      661 gcacgcgcca gcacaagcgg tagccgcggg ccggagggcc gagccgctac tcgcgcggga
      721 tcgcgcgcag ccggtagccg aagcggtgcc gcgactcgat caggtcgccg atctcggact
      781 tgatcagcgc gcggcggacg cggcggacca gctgcttgac gtggttctcg ctcggctcgc
      841 gggtgtccca cgacaggtcg gcgatcagct ccgaggaccg gacgaagccg cggaccagct
      901 cgggctggtg gtgctcgccg gccatgcgct tgatcatcag cgccatcagc tcgaactgcg
      961 tcgtggtgag ctggacctgc ttgccatcga cctcgatcag gccgccgccg ccgccggtcg
     1021 gctcgtgcat cttgaacgac atcacgggca ggccgacgtc ggtgtggtcc tcctcggcga
     1081 gcggctcgat cgggaacgtc ggggcggtgc ggaccgctct gcgttcgacc ggcttgatcg
     1141 tcgcggtcag gatgcggccg acccgcggcg gcggcagccg cgcgacgtcc tcgacgatgt
     1201 agaacgcgat gtggccgaac cgcacccggt cgccctcgtg catcgcgacc ggcgtcgaga
     1261 tcagcttgtc gtccaggaac gtgccgttgg ccgaaccgag gtcgcgcacg gtccactcgt
     1321 cgttgtccag gatcacgtgg gcgtggtggc gagacaccga cggctcgagg atcgccaggc
     1381 cctggccatc gacctggcgg ccgatcagcg tccgcgggtc gaggcggtgc ggccggcccc
     1441 agagatcgat cagcgccgcc atcgtgggct gtgcgccgtg cagctggatc tgctccggca
     1501 tgatcttgat ggggctcgac agctcgtcac gacattcttc gcacagcacg ccgacctcgg
     1561 cggggcgctg gcgacacacc gggcacatca cgtggacagt ttatcatcag cgccatcacc
     1621 aagcgcggcc gccgtgaacg agggcgcgca ttccacgccg ccgatcgcgc tgtgctggag
     1681 tccggcggtg cggtcgcggg aaagtgtatg tgcaaagcgc cgcatggagc gtgcaggagt
     1741 gcatgcgatc cgtggtgcag cacgatccgc cagccatggc tcactgtcgc gccggactac
     1801 gaattccacg cagcggccgg cagtccaccg gcccgctact cccacgccgc gtagcgctgg
     1861 ccgaggaccg gcggcgacgc gcggttcggc gcgaccgcga gctcgaacgg gaacgaccgc
     1921 ttggcgatgc cgtcgtccgc ggcggtcatc acctggaagt gcaggtgcgg cgtcgacgag
     1981 aacccggtgt tgccggacag cccgagctcc tggccgcgcc gcaccgcctg gccgggagtg
     2041 acctcgacgc cgctcggcgc caggtgcatg tactcgccga gcgtgccgtc gctgtgctgg
     2101 acgagcacga agttggcgcg ccggtcgtcg aggaactccg gggtggtgcc cgagccctgc
     2161 gcgccggcgt tgatcgcgac cacgaccccg gggcgcgccg cggtcaccgg ggtcgcgacc
     2221 gggcagttga agtcgaccgc gtactcgttg gccccgcggt gcgagaactc gccgtggaag
     2281 ccctgcagca cc
//
LOCUS       DASVOR010000958         1821 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_389253,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000958 DASVOR010000000
VERSION     DASVOR010000958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389253"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF516_18560"
     CDS             complement(<1..491)
                     /locus_tag="VF516_18560"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8109744.1"
                     /translation="MAQPVQNVFQALDQALAAAVATAGRSVVQVGRGHGHAGTGIVWA
                     DDLVISSSFHTPDRTKVGIPVPQGNGDAGDVEVREAEVIGRDPGTDVALLRVTGGGLT
                     PATFRDLGGLAAGNLALALGRPGRTVRASLRAVGVLGPAVRTPHGGKLDRYVESDRQI
                     PRG"
     gene            716..1339
                     /locus_tag="VF516_18565"
     CDS             716..1339
                     /locus_tag="VF516_18565"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8109745.1"
                     /translation="MTRNPNLPIVAVGDPIKVALLTDDPLLRAGLSSLLAQLGSIDVV
                     ERDRAEVALWDAGVDPSKTLSRLSELRSLPMPVVAVVGDPAHVAPALAAGARGVVLRD
                     QVGPGIHAALAAVRSGLTVMDTQLASSLVPSQPPREPKGRGELTERERQVVQLLAEGL
                     SNKLIADRLGISDHTAKFHVNGVMMKLGSSTRTEAVVEAMRRGLIRL"
     gene            complement(1254..1709)
                     /locus_tag="VF516_18570"
     CDS             complement(1254..1709)
                     /locus_tag="VF516_18570"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEX8109746.1"
                     /translation="MASSEMAEHVAKLYRAIYAVVRRIPAGHVATYGQVAELAGIPGG
                     GRVAGAAMKVSRPGDRLPWQRVIGKAGPNRGRIAIHDPVGAAVQRQLLDEEGVEINDS
                     GLVALDRYGWLPGDGSRRPRRATAGSGRGASPRRRPRSASTSRASSSRR"
BASE COUNT          241 a          667 c          656 g          257 t
ORIGIN      
        1 aacccgcgcg ggatctggcg atccgactcg acgtagcgat ccagcttgcc gccgtgcggg
       61 gtgcgcaccg ccggaccgag cacgccaacc gcgcgcagcg acgcgcgcac cgtgcggccc
      121 gggcggccga gcgcgagcgc caggttgccc gccgccaggc cgccgaggtc gcgaaacgtc
      181 gccggggtca gcccgccgcc ggtcacccgc agcagcgcga cgtcggtccc cggatcgcgg
      241 ccgatgacct cggcctcgcg gacctcgacg tcccccgcgt ccccgttgcc ctgcggcacc
      301 gggatgccta ccttggtgcg gtccggagta tggaagctgg agctgatcac gaggtcatct
      361 gcccacacga tgccggtccc ggcgtggccg tggccgcggc cgacctggac gaccgatcgc
      421 ccggcggtcg ccaccgcggc ggcgagcgcc tgatcgaggg cctggaagac gttctgtact
      481 ggttgcgcca tggagactct ccttctcgcc gacgagcttg cgccggtgga ccgcggccgc
      541 atatccgcat ttcggcgagg ttctcgtcgg aaaatcggtg aggcgcgccc gcgcccccgg
      601 tgctccgggg gtccggccct ccccatccgg gtagggttga cctggctgct tgccgtgacc
      661 acactgcacc taacgtacgc gtcagctttc ccatcgacgc ggcgatgaaa gaaccatgac
      721 tcgcaatccc aacctgccga tcgtcgctgt aggcgacccg atcaaagtgg cgttgctgac
      781 cgacgatccg ctgctgcgcg cggggttgtc gagcttgctc gcgcagctcg gctcgatcga
      841 cgtggtcgag cgcgatcggg cggaggtggc gctgtgggac gccggcgtcg accccagcaa
      901 gaccctgtcg cggctgtccg agctgcgaag tctcccgatg ccggtcgtcg ccgtggtggg
      961 cgatcccgca catgtcgctc ccgcgctcgc ggccggcgcg cgcggggtgg tgctgcgcga
     1021 ccaggtcggc ccgggcatcc acgcggcgct ggccgcggtc cgcagcggcc tgaccgtgat
     1081 ggacacccag ctcgcgtcga gcctcgtgcc cagccagccg ccgcgcgagc ccaagggccg
     1141 cggcgagctg accgagcgcg agcgccaggt cgtgcagctg ctcgccgagg gcctgtcgaa
     1201 caagctgatc gccgatcgcc tcggcatcag cgatcacacc gcgaagttcc acgtcaacgg
     1261 cgtgatgatg aagctcggct cgtcgacgcg gaccgaggcc gtcgtcgagg cgatgcgccg
     1321 cggcctgatc cggctgtagc gcggcgcggc cggcggctgc cgtcgccggg cagccagccg
     1381 tagcggtcga gcgcgaccag gccgctgtcg ttgatctcga cgccctcctc gtcgagcagc
     1441 tggcgctgga ccgcggcgcc gaccggatcg tggatcgcga tccggccgcg gttggggccg
     1501 gccttgccga tcacccgctg ccacggcagc cggtcgcccg gccgggagac cttcatggcc
     1561 gcgccggcga cgcggccgcc gccggggatg ccggcgagct cggcgacctg gccgtaggtc
     1621 gcgacgtggc cggccgggat ccggcgcacc acggcgtaga tcgcccggta gagcttcgcg
     1681 acgtgctcgg ccatctcgct ggacgccatc gcgaggcaac gctaccaccg gtgcatgaag
     1741 atccgcgcct tgtacttcgc cgtgttccgg gagcagatcg gacgcgacga ggacgtggtc
     1801 gagatcgccg ccggcgcccg g
//
LOCUS       DASVOR010000959         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_360390,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000959 DASVOR010000000
VERSION     DASVOR010000959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360390"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..462
                     /locus_tag="VF516_18575"
     CDS             112..462
                     /locus_tag="VF516_18575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109747.1"
                     /translation="MPNEFLHRGFFAQLSHAERSLYFFLVLAGDRNGVSFYAHDRICA
                     ALELTLDDYLVVRDRLIDLDLIAFDGTRFQVLSLPAAPSVPPPRPLVTQDDFEDHDPA
                     TIRRLLQASPDRRR"
     gene            complement(1274..>1912)
                     /locus_tag="VF516_18580"
     CDS             complement(1274..>1912)
                     /locus_tag="VF516_18580"
                     /inference="COORDINATES: protein motif:HMM:NF025596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Druantia anti-phage system protein DruA"
                     /protein_id="HEX8109748.1"
                     /translation="EIASSVAGRAIVRVPNLVLLGTTSLYGVGSSQYNRIKIPAEHIG
                     GTRGDSLRYEEIGRSEAFGTSHYSEETVAALVKLVQQADAQRVNSVFGEGASPKLRKV
                     KHALDLLGLPSELLLRHHRPRVVYVVSLIRNLRDHLIGLDKKPQYFVALDGAATTAKI
                     GAWWRERWLRNRIMSDEVLEEVSRHTRVVPITHGARVPWQPGQNQQSLFADA"
BASE COUNT          330 a          656 c          593 g          333 t
ORIGIN      
        1 gacgtgatgc cgacgaagcc tgacacgcca gccggcgctg gccgaacgcc gacgcatcgc
       61 acaccgccgc gaccggatcg gattcgccgc atcacaggcg gcttcgcctt cgtgcccaac
      121 gagttcttgc accgcggctt cttcgcacag ctcagccacg ccgagcgctc gctgtacttc
      181 ttcctggtcc tcgcaggcga ccgcaacggc gtcagcttct acgcccacga ccggatctgc
      241 gccgccctcg agctcacgct cgacgactat ctcgtcgtgc gcgatcgcct catcgacctg
      301 gacctcatcg ccttcgacgg cacccggttc caggtcctgt cgctgccggc ggcgcccagc
      361 gtgccgccgc cgcgccccct ggtcacccag gacgacttcg aggaccacga ccccgcgacc
      421 atccggcgcc tcctccaggc ctcgccagat cgacgccgct aaccgccgcc gccgccggat
      481 ccagtcgatc catccgatcc agcggaacgg atccagacgg acgggttaca cccgcctggg
      541 atccagacgg atccggatcc cggcatccgg cccaggtggg tcacgatccc gccgcgccgc
      601 cgccacctgc gtggatccac gccagcgccc ggaatggatc aacccgaagg cgccgccaaa
      661 ggcgaaagtg atcaacgctc ggatcgactc gtcgcctggt gttcctgatg gagagcaacc
      721 agcagtgcgt cgcgcggctt cgagagcggc tggtgctgga gtggtcctca acgaggtgcg
      781 ccgactgctc gccgcgtcgg tatctacacg acaactcgca ctgatcggac ccgccgcgct
      841 tgcgcagagt gcgcttgcgc gcgcagggag tgttgatggg atcccactaa cgacggacgc
      901 tgcggagtgg cgacagggac gcggcatttt ggtaacgaca gcggcctcgt tcaaggggct
      961 cgaagcggac gttatccttc tgtacgactt gagcgggttt agtgaagcat tttcccgagc
     1021 ccacctatat gtcgcatgga cccgagctcg tatgcgcctt ttcatctttg tctatggaga
     1081 cgaggcccgc gtcgccgtcg aggcggcact cgctgatgcg cccggcgcgg gatgaaagcg
     1141 ttggcctccg acggattggt ttgcagccgc actcgatcag gagtggaggt taagggttcg
     1201 atgtacctac cactgcagac tgccttcgtc gccgggacct ggttcacgcg aggtcgaggc
     1261 gcccttggaa gggtcacgcg tcggcgaaga gggattgttg gttttgcccc ggctgccagg
     1321 gcacgcgcgc gccatgagtg atcgggacga cccgtgtgtg ccgagatacc tcttcgagca
     1381 cctcatcaga catgatgcgg ttacggagcc accgctccct ccaccacgcg ccgatcttcg
     1441 ccgtcgtcgc agcaccatcg agcgcgacga agtactgagg cttcttgtca aggccgatga
     1501 ggtgatctcg caagtttcgg atcaagctca cgacgtagac gacgcggggc cggtgatgac
     1561 gcaacagcag ctcactgggc aggccgagca gatcaagcgc gtgcttcacc tttcggagct
     1621 tcgggctcgc gccctcgccg aaaacgctgt tcacgcgctg cgcgtcggcc tgctgcacga
     1681 gcttgacaag ggcagcgaca gtctcctccg agtaatgcga tgtcccgaac gcctcggacc
     1741 gcccgatctc ctcgtagcgc aaagagtcgc cccttgtccc cccgatgtgc tcggcaggga
     1801 tcttgatgcg gttgtattgg ctcgaaccga cgccatagag tgacgtcgtc ccgagaagca
     1861 cgaggttcgg cacgcgcacg atagcccgtc cggcgacgga ggacgcgatc tc
//
LOCUS       DASVOR010000960         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_97856,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000960 DASVOR010000000
VERSION     DASVOR010000960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97856"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VF516_18585"
     CDS             complement(<1..478)
                     /locus_tag="VF516_18585"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8109749.1"
                     /translation="MRTPSLFTAVSVTLCLSLASHPRALVADPTPPLAPEIPAQFTRP
                     TDAADYERREVMVAMRDSVKLHTVILVPRGARRLPIILTRTPYNASKRAENTVSNRLA
                     ATLGVSDDQFSDGSFIRVFQDVRGKYGSEGDYVMTRPLRGPLNNSKVDHSTDTYDTI"
     gene            680..1081
                     /locus_tag="VF516_18590"
     CDS             680..1081
                     /locus_tag="VF516_18590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109750.1"
                     /translation="MTRALAFVFVVGCIHPSGAPPDDSGSVCGDLVYTRDGKCLPPDE
                     VRTIHVTWTVSGAPASATSCAAAPDLRLDLRSRSGGSVGFEPVPCVEGKFTVDRLSTD
                     YDTVQLGRASDRSLPSAMIDAAGNAMLNLPY"
     gene            1091..1651
                     /locus_tag="VF516_18595"
     CDS             1091..1651
                     /locus_tag="VF516_18595"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HEX8109751.1"
                     /translation="MAAILAIAGSLREKSFNAMLLRAAAELAPAGTTVEAASIKDIPL
                     YDGDVEAAAGIPPAVHALKERIAAAPGVLLVTPEYNNSLPGVFKNAIDWTSRPPADLG
                     RVYGGKPIGVIGATPGMGGTSLAQAAWLPVLRTLGTLPFFGARVQVSSAAKVFDDHGR
                     LVDPTVRGQLEKYMAAFAAFVDRHAR"
BASE COUNT          264 a          648 c          710 g          264 t
ORIGIN      
        1 cgatcgtgtc gtaggtgtcg gtcgagtggt cgaccttgct gttgttgagc gggccgcgca
       61 gcgggcgggt catgacgtag tcgccctcgg agccgtactt gccgcggacg tcctggaaga
      121 cccggatgaa gctgccgtcg gagaactggt cgtcgctgac ccccagggtc gcggcgagcc
      181 ggttggacac cgtgttctcg gcgcgcttgc tggcgttgta cggcgtgcgg gtcaggatga
      241 tcggcagccg gcgggcgccg cgcggcacga ggatcacggt gtggagcttg acgctgtcgc
      301 gcatcgcgac catgacctcg cggcgctcgt agtcggcggc gtcggtcggc cgcgtgaact
      361 gcgccgggat ctcgggcgcc agcggcggcg tcgggtcggc gaccagggcg cgcgggtgcg
      421 aggcgagcga caggcagagc gtcacggaca ccgcggtgaa caggctgggc gtgcgcatgc
      481 cgcaggacta ttgccccaaa cggcggcggt ggggaacgtg caatgcgcac gcggagcgcg
      541 cggcggggcg cgatcgcggc agcgcggccc gctccgccac cggcagcgtt gccacgcgcc
      601 gtgacgttca tcggggcaaa acgccgaccg gtcggtagat cctctgccgg agggcggcgg
      661 ctttgctacg cttgcgggca tgacacgtgc gcttgcgttc gtgttcgtgg tcgggtgcat
      721 ccatccgtca ggcgcgccgc ccgacgatag cggcagcgtg tgcggggacc tggtctacac
      781 gcgcgacggc aagtgcctgc cgcccgacga ggttcgcacc atccacgtca cctggacggt
      841 gagcggcgca ccggcgagcg cgacgagctg cgcggcggcg cccgatctcc ggctcgacct
      901 gcgaagccgg agcgggggca gcgtcgggtt cgagccggtg ccgtgcgtcg agggcaagtt
      961 cacggtcgac cggctgtcga cggactacga tacggtccag ctcggccgcg cctccgatcg
     1021 gtcgctgccc agcgccatga tcgacgccgc cggcaatgcg atgctgaacc tgccgtatta
     1081 agatttcgag atggctgcca tcctcgcgat cgcagggagc ctgcgggaga agtcgttcaa
     1141 cgcgatgctg ctgcgcgccg ccgcggagct cgcaccggcc gggaccaccg tcgaggctgc
     1201 ctcgatcaag gacatcccgc tgtacgacgg cgatgtcgag gcggcggccg ggatcccgcc
     1261 ggcggtccac gcgctcaagg aacggatcgc ggcggctccc ggcgtcctcc tggtcacgcc
     1321 ggagtacaac aactcgctgc cgggtgtgtt caagaacgcg atcgactgga ccagccggcc
     1381 gcccgcggac ctcggccggg tctacggcgg caagccgatc ggtgtgatcg gcgcaacgcc
     1441 cgggatgggc ggcaccagcc tcgcgcaggc ggcctggctc cccgtgctgc gcacgctcgg
     1501 cacgctgccg ttcttcgggg cgcgcgtcca ggtatccagc gcggccaagg tgttcgacga
     1561 ccacggccgg ctcgtcgatc cgacggtgcg cggccagctc gagaagtaca tggcggcgtt
     1621 cgccgcgttc gtcgaccgcc acgcgcgctg acgcggcggc cggcgtggcg gacgcacgat
     1681 cggtgtgcgc cggcgcgcac tccgggtggc ccggtcgcta ggatgggggc atgagcgctg
     1741 acaagccacc cgcacatccc gcgaagcccg cggccaaggc cgacgccgac tccaagcccg
     1801 ccgccaagcc ggcggcgaag cccgccgcgc agccacccgc gctggacgag cggccgctca
     1861 agttcgccga cctcgcggcg tacgcc
//
LOCUS       DASVOR010000961         2455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447781,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000961 DASVOR010000000
VERSION     DASVOR010000961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447781"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..862)
                     /locus_tag="VF516_18600"
     CDS             complement(<1..862)
                     /locus_tag="VF516_18600"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8109752.1"
                     /translation="MALPLGGLLAGCAAPDPVDDSASTVARADGDRDDHDRDDRDAIH
                     KVKHVIVIMQENHSFDNYFGALAYAPGSPYHRPHGHHHDDGDGDRDDRTGCPPGDHHC
                     VDGLSCEFDHDGAFHCANANLDDDGSVVHAFHDPNRCPAPDLDHGWVSTHREANFDHP
                     NDVRRHAPMDGFVRINDATEQHDNGVESATEDETIGFYNQDDLPFYYDLAQNFAISDR
                     MFASVLGPTFPNRAYLLAATSFGHLTTSDTFPIGDGYKPITGTIFDLMEHNNVSWADY
                     FQDVPQGGSFR"
     gene            complement(1081..>2455)
                     /locus_tag="VF516_18605"
     CDS             complement(1081..>2455)
                     /locus_tag="VF516_18605"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8109753.1"
                     /translation="PYRPPAAGRTGCRGTDHRCVDGLSCAVDAGGALRCSNANLDDDG
                     STVVAFHDPNRCPAPDLDHGWASTHREVNFAQPNATRRQPLLDGFVRVNDETEQHDGE
                     ETPTEDETMGFYTQDDLPFYYDLAQHFAISDRFFSSMLGPTFPNRAYFAAATSFGHVS
                     TADQIPPLEGYRPVGGTIWDLLDRNGVSWADYYQDLPQAASFRPLGSTVIDPHFQLLP
                     VFLAQAAGLGDLPSVSFVDPDFGALGTATENDEHPPTDIQRGQAFVAQVVNAVRNGPH
                     WADSVIFLVYDEHGGFYDHVPPPPAVAPDPIQPGQCEDLSNPPASLQPGGGAGCSSNL
                     KGDPDTSLAEAIALCPALAADPTGPYPAGCARFDQLGVRVPFLAVSPFSRPQYVSHAI
                     ADHTSILAFIETAFLPRVHGHRQHLTRRDQEADDLRDLFDFAGAPSLGTTVGVAAPPA
                     NDCTPLL"
BASE COUNT          361 a          749 c          947 g          398 t
ORIGIN      
        1 gccggaagct gccgccctgg ggcacgtcct ggaagtagtc ggcccacgac acgttgttgt
       61 gctccatcag atcgaagatc gtgccggtga tcggcttgta gccgtcgccg atcgggaacg
      121 tgtcgctggt ggtcaggtga ccgaacgagg tcgccgccag caggtacgcg cggttgggga
      181 acgtcgggcc gagcaccgag gcgaacatgc gatcgctgat cgcgaagttc tgggccaggt
      241 cgtaatagaa cggcaggtcg tcctggttgt agaagccgat ggtctcgtcc tcggtggccg
      301 actcgacgcc gttgtcgtgc tgctcggtgg cgtcgttgat ccggacgaag ccgtccatcg
      361 gcgcgtggcg gcgcacgtcg ttggggtgat cgaagttggc ctcgcggtgg gtgctgaccc
      421 agccgtgatc gaggtcgggc gcggggcagc ggttggggtc gtggaacgcg tggacgacgc
      481 tgccgtcgtc gtcgaggttg gcgttggcgc agtggaacgc gccgtcgtga tcgaactcgc
      541 agctcagacc gtcgacgcag tggtgatcgc ccggcgggca gccggtgcga tcgtcgcgat
      601 cgccgtcgcc gtcatcgtgg tgatggccgt gcggccggtg atacggactg ccgggcgcat
      661 aggccagggc gccgaagtag ttatcgaacg agtgattctc ctgcatgatc acgatgacgt
      721 gcttgacctt gtggatcgcg tcgcgatcgt cgcggtcgtg gtcatcgcga tcgccgtcgg
      781 cgcgcgcgac cgtcgacgcg ctgtcgtcga ccggatccgg cgccgcgcag ccggcgagca
      841 ggccacccag aggtaaggcc aaacatagac acatccatcg agctggctcc cgtgcgctca
      901 tcagtgactc ccgtggtgat gggcgcgcat catagcgatc gcgcctccgg acacacgccg
      961 cgatcctgcg acatttttgg ggaacggcgc gtgatgcaga tcgctgccgg agacgaccga
     1021 ggcgtcgcgg cgtcacgctg tcatggcgat gtgatgtgag gacgtcgcga tgaatcggct
     1081 ttatagcagc ggtgtgcagt cgttggccgg tggcgccgcg acgccgacgg tcgtgccgag
     1141 cgagggcgcg ccggcaaagt cgaacaggtc gcgcaggtca tcggcctcct gatcgcgccg
     1201 ggtcaggtgc tggcggtggc cgtgcacgcg cggcaggaac gcggtctcga tgaacgcgag
     1261 gatcgacgtg tggtcggcga tcgcgtgcga gacgtactgc ggcctcgaga acggcgacac
     1321 cgcgaggaac ggcacacgga cgccgagctg atcgaaccgg gcgcacccgg cggggtacgg
     1381 cccggtcggg tcggccgcca gcgcggggca cagcgcgatc gcctcggcca ggctggtgtc
     1441 gggatcgccc ttgaggttgc tgctgcaccc ggcgccaccg ccgggctgga ggctggccgg
     1501 cgggttcgac aggtcctcgc actggccggg ctggatcgga tcgggcgcga ccgcgggcgg
     1561 cggcggcacg tgatcgtaga acccgccgtg ctcgtcgtag acgaggaaga tcaccgagtc
     1621 ggcccagtgc gggccgttgc gcacggcgtt gaccacctgg gcgacgaacg cctggccgcg
     1681 ctggatgtcg gtcggcgggt gctcgtcgtt ctcggtcgcc gtgccgagcg cgccgaagtc
     1741 cggatcgacg aacgataccg agggcaggtc accgaggccg gccgcctggg cgaggaacac
     1801 cggcagcagc tggaagtgcg gatcgatcac cgtcgagccg agcgggcgga agctcgccgc
     1861 ctgtggcaga tcctgatagt aatcggccca cgacacgcca ttgcggtcga gcaggtccca
     1921 gattgtcccg ccgaccggcc ggtagccctc cagcggcggg atctggtcgg cggtcgagac
     1981 gtggccgaac gaggttgccg ccgcgaagta ggcgcggttg gggaacgtcg ggcccagcat
     2041 cgaggagaag aaccgatcgc tgatcgcgaa gtgctgcgcc aggtcgtaat agaacggcag
     2101 gtcgtcctgg gtatagaagc ccatggtctc gtcctcggtc ggcgtctcct cgccgtcgtg
     2161 ctgctcggtc tcgtcgttga cccggacgaa gccgtcgagc agcggctggc ggcgcgtcgc
     2221 attgggctgt gcgaagttga cctcgcggtg cgtgctggcc cagccgtgat cgaggtcggg
     2281 cgcggggcag cggttggggt cgtggaatgc gaccaccgtg ctgccgtcgt cgtcgaggtt
     2341 ggcgttgctg cagcgcagcg cgccgccggc gtcgaccgcg cagctcaggc cgtcgacgca
     2401 gcggtggtcg gtgccgcggc agccggtgcg gccggcggcg ggtggccggt acggg
//
LOCUS       DASVOR010000962         1586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_565281,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000962 DASVOR010000000
VERSION     DASVOR010000962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1586
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565281"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..280
                     /locus_tag="VF516_18610"
     CDS             56..280
                     /locus_tag="VF516_18610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109754.1"
                     /translation="MIRRAAALPARPRDPGRVRDQRAPQRDPQLTVYAGWPLFPSAAY
                     DAAYFAMVVAFYGPVAYWLVKRDDTGRAAP"
     gene            complement(296..862)
                     /locus_tag="VF516_18615"
     CDS             complement(296..862)
                     /locus_tag="VF516_18615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEX8109755.1"
                     /translation="MYCASSSRIDEVYFDATERLARALVAAGAEVVYGGGSSGLMGRL
                     ADTVLDLGGKIKGIMPRFMNEVEWAHRRLTDLELTDTMHERKARFLEGVYGLVALPGG
                     TGTLEELLEAITLKRLGQLNRPIVILNTRGFYDPLRELLERTVRERFMHEKHLELWTF
                     VDEPEQVLPAMAHAPDWPADALAIATLR"
     gene            complement(927..>1586)
                     /locus_tag="VF516_18620"
     CDS             complement(927..>1586)
                     /locus_tag="VF516_18620"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX8109756.1"
                     /translation="SYGGSNLCSGSGSYDRAVDPRSVATERADSDLTHFVGFVLDDVN
                     RTWADQLPGYQDTRLVLFRNAIRSACGTATTAVGPFYCPLDHKVYIDLAFYDELRRRF
                     GAPGDFAQAYVIAHEVGHHVQNLHGLLGRGEVSSVPIELQADCLAGAWARSADTRGLV
                     EVGDVDEALNAASQIGDDTIQRKATGHVQPETFTHGTAAQRSSSFRRGYQGGPAACEI
                     R"
BASE COUNT          218 a          568 c          561 g          239 t
ORIGIN      
        1 tcaacgatcc ggtcgggcac tacctggaga tcctcacgcg tccctacggc agcggatgat
       61 ccggcgagcc gcggcgttgc cggctcgacc gcgtgatcca ggtcgtgttc gcgatcaacg
      121 cgctccgcaa cgcgacccgc agctcaccgt ctacgccggg tggccgctgt tcccgtcggc
      181 cgcgtacgac gccgcgtact tcgcgatggt ggtcgcgttc tacggcccgg tcgcgtactg
      241 gctggtcaag cgcgacgata ccggccgcgc ggcgccgtga cctgcgcggc cggggtcacc
      301 ggagcgtggc gatcgcgagc gcgtcggcgg gccagtccgg cgcgtgcgcc atggccggca
      361 gcacctgctc gggctcgtcg acgaacgtcc acagctcgag gtgcttctcg tgcatgaagc
      421 gctcgcggac ggtgcgctcc agcagctcgc gcagcgggtc gtagaacccg cgggtgttga
      481 ggatgacgat cggccggttg agctgcccga gccgcttgag cgtgatcgcc tcgagcagct
      541 cctccagcgt gccggtgccg cccggcagcg cgaccagccc gtagacgccc tcgaggaacc
      601 gcgccttgcg ctcgtgcatg gtgtcggtga gctcgaggtc ggtgaggcgc cggtgcgccc
      661 attcgacctc gttcatgaac cgcggcatga tgcccttgat cttgccgccg aggtccagga
      721 cggtatcggc gagccgaccc atcagcccgc tcgagccgcc gccgtagacg acctcggcgc
      781 cggcggcgac cagcgcccgg gccaggcgct cggtggcgtc gaagtacacc tcgtcgatcc
      841 tcgagctcga ggcacagtac acgcagatcc gcatcgcgca ggctccctga tccagtccgc
      901 gcgcagcata gcccacgcgc cggcggtcag cggatctcgc acgccgccgg gccgccctga
      961 tagccgcgcc ggaacgagct cgagcgctgc gccgccgtgc cgtgggtgaa cgtctcgggc
     1021 tggacgtggc cggtcgcctt gcgctggatc gtgtcgtcgc cgatctgcga ggcggcgttg
     1081 agcgcctcgt cgacgtcgcc gacctcgacc aggccgcgcg tgtcggccga ccgcgcccac
     1141 gcgccggcca ggcagtcggc ctggagctcg atcggcaccg agctgacctc gccgcggccg
     1201 agcagcccgt gcaggttctg gacgtgatgg ccgacctcgt gcgcgatcac gtacgcctgt
     1261 gcgaagtcgc ccggtgcgcc gaaccgccgg cgcagctcat cgtagaacgc caggtcgatg
     1321 tagaccttgt gatcgagcgg gcagtagaac ggtccgaccg ccgtcgtggc agtgccgcac
     1381 gccgagcgga tcgcgttgcg gaacagcacg agccgcgtgt cctgatagcc cggcagctgg
     1441 tcggcccagg tgcggttgac gtcgtcgagc acgaagccga cgaagtgcgt cagatcgctg
     1501 tccgcccgct cggtggcgac cgaccgcgga tcgaccgcgc ggtcgtagct gcccgagccg
     1561 ctgcacaggt tgctgccgcc gtagct
//
LOCUS       DASVOR010000963         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_185210,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000963 DASVOR010000000
VERSION     DASVOR010000963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185210"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..1251)
                     /locus_tag="VF516_18625"
     CDS             complement(10..1251)
                     /locus_tag="VF516_18625"
                     /inference="COORDINATES: protein motif:HMM:NF033550.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEX8109757.1"
                     /translation="MRITKLLRLLLGLEETRVVDVGFDYAGLVVDVAPTWRNPRCSGC
                     GESGAGYDRMRDRRWRHLDVAGMEFHLRYDTRRVDCPRCGVKVERVPWAEPGSWFTRP
                     FEDHVGYLAQRCDKTTVSAMMRVAWDTVGAIIQRVVARRQDPDALDGLTSIGVDELSY
                     RRHHEYITVVVDHRRGHVVWAQPGKNADTLKAFFEELGPERCAKLEAVTIDMSAAYIK
                     AVTECSPQAQLIFDRFHVQRLAQDAVDEVRRDEVRAATTVEEGKQLKGTRWSLLKSFW
                     NLSLFDSARLSQLQRDNKRLYRAYLLKDALVRVLDCRDEWLARVKLDQWIRWARRSRL
                     EPFKRIAVTIREHTEGIIAYVTSGLSNGRTEGLNGKARTITRRAYGFHSAHGLIALLK
                     LCCSGIHLDPVHIDPGSTHKT"
     gene            complement(1452..>1768)
                     /locus_tag="VF516_18630"
     CDS             complement(1452..>1768)
                     /locus_tag="VF516_18630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8109758.1"
                     /translation="FELVDGSIAKAISGSWTTEARRLIEGSDCVIVLCGEQTHQAKGV
                     VTEVQIAQELGKRYFLLQGTRSGRPSRPPNARATDKIWAFRWPTVSALLDGKTPPSDA
                     GR"
BASE COUNT          298 a          594 c          560 g          316 t
ORIGIN      
        1 ggctctcccc taggttttgt gggtggatcc agggtcgata tggaccggat cgagatgaat
       61 tcctgagcag cagagcttga gaagggcgat gagtccgtga gcactgtgga atccataggc
      121 gcgacgcgtg atggtacgag ccttcccatt gagtccctcc gtgcggccgt tcgagagccc
      181 agaggtgacg tacgcgatga tcccctcggt gtgctcgcgg atcgtgaccg cgatgcgctt
      241 gaagggctcg agccgggagc ggcgcgccca tcggatccac tggtcgagct tgacgcgggc
      301 gagccactcg tcccggcagt cgagaacgcg gaccagcgca tccttgagca ggtacgctcg
      361 gtagaggcgc ttgttgtccc gttgcagctg cgagagccgc gccgagtcga acagcgacag
      421 gttccagaag cttttgagca agctccaacg tgtgcccttg agctgctttc cttcctcgac
      481 ggtcgtggcg gcacgaactt catcgcggcg aacctcgtcg accgcatcct gtgccagccg
      541 ctgaacatgg aaccggtcga agatgagctg tgcctgcggc gaacactcgg tcacggcctt
      601 gatgtacgcg gcggacatgt cgatcgtgac cgcttccagc ttggcgcagc gctccggtcc
      661 gagctcctcg aagaacgcct tgagcgtgtc ggcgttcttg cccggctgcg cccagaccac
      721 gtggccgcgc cgatggtcga ccaccacggt gatgtactcg tgatgtcgtc ggtaggacag
      781 ctcgtcgacg ccgatggatg tgaggccgtc gagagcatcg gggtcctggc ggcgggcaac
      841 gacgcgctgg atgatcgcac cgacggtgtc ccatgccacg cgcatcatcg cgctgaccgt
      901 cgtcttgtcg cagcgctggg ccaggtatcc gacgtggtcc tcgaacggtc gcgtgaacca
      961 cgaaccaggc tcggcccatg gcacccgctc gaccttgacg ccacacctcg gacagtcgac
     1021 gcgccgggta tcgtaccgca ggtggaactc catgccggcc acatcgaggt gcctccagcg
     1081 acggtctcgc atgcggtcgt agccagcgcc gctctctccg catcccgagc accgcgggtt
     1141 gcgccacgtc ggtgctacgt cgacgaccag cccggcgtag tcgaacccca catcgacgac
     1201 acgtgtctct tccaatccga gcaggaggcg gagtagcttg gtgatgcgca cggcggaacc
     1261 tcggtgtcgt acttggcgta ggaaccagca cggtaccgcg acccgccggg cgcacgcttt
     1321 ccatcacccc gcaaacggcc tggaggccgc cgcttcccca cctacgacgc gatctgcgcg
     1381 ttcccatcac ccactaaacc aggtggagag ccattcatcc cgcctcgatg tccgagcgcc
     1441 gtgagacgcg gtcagcgccc cgcatcgctc ggcggcgtct ttccatccag aagggcgctg
     1501 acggtgggcc agcggaacgc ccagatcttg tcggtggccc gcgcattggg aggccgcgac
     1561 ggtcttccac tgcgggtacc ctgcagcaag aagtatcgtt tccccagctc ctgcgcgatc
     1621 tggacctccg tcacgacccc cttcgcctga tgggtttgct cgccgcacaa cacgatcacg
     1681 cagtcactcc cctcgatgag tctgcgcgcc tcggtggtcc agctaccgga gatcgccttc
     1741 gcgatcgagc cgtcgaccag ctcgaagg
//
LOCUS       DASVOR010000964         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_565289,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000964 DASVOR010000000
VERSION     DASVOR010000964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_565289"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VF516_18635"
     CDS             <1..329
                     /locus_tag="VF516_18635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109759.1"
                     /translation="LAALHGGAAPAPRLVEAVSRAVAASRLAATKHVVEAPLLRAVEL
                     DLAADTARNRALHDRIHLAFAVGQRWANLDRFVEWIYAELFEMPLGDAALGLDVPDPF
                     PLTAAG"
     gene            complement(340..2052)
                     /locus_tag="VF516_18640"
     CDS             complement(340..2052)
                     /locus_tag="VF516_18640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8109760.1"
                     /translation="MAHRKSEPVLPIRPRTRSRERLGPSPADSLIQSPAPPASDTPRR
                     VIRLLLLRTLVVTAVLGLSLWILTTAEQVPESAVWLQSGIIAATYATSIVFGVMVRRG
                     FSPRQVARPMHAADLAVASLLIYATGGAESPYIFLSALSVVGAGALSYRGGAVVITLA
                     SISLLIVVSLLAWTQAIALPALSQVRPWEQSGVDLVRTLGINLAALIGVGALSFIFGD
                     QLQRGVETLATTRKAAADLLTVHEDIVRSLASGLITIAPDGIILTANSAAADILGQPP
                     ALAGQPVDQLMPGLSALIATGHGELRRADLQLAADLTIGVTVSPLRDVKNQVLGRVVN
                     FQDLTELRRLEAQSRRSERLAMIGQLAAGIAHEIRNPLASISGSIELLRQGPSASDDD
                     RTLMAIVHREVQRLNVLIGDLLDYANPRPPQPVDFDLGVMVEETLQVARREQAFASVE
                     MAFAVDRPLPLHADPAKLRQVLWNLLRNAADAAALGGRHVHVDARRGLDATTLVVSDD
                     GPGIPADQLARIFDPFFTTKSKGTGLGLATCHAIVAEHGGHIDAASEPGTGTQMTVSL
                     PRER"
     gene            complement(2056..>2206)
                     /locus_tag="VF516_18645"
     CDS             complement(2056..>2206)
                     /locus_tag="VF516_18645"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8109761.1"
                     /translation="YEEEVDAAVSALTSLIEPVMMAFLGVVVGGLIIAMYLPIFKLAG
                     NIGTG"
BASE COUNT          309 a          764 c          847 g          286 t
ORIGIN      
        1 agctcgccgc gctgcacggc ggtgctgccc ccgcgccgcg gctggtggag gcggtgtcgc
       61 gcgcggtcgc cgccagccgg ctcgcagcga ccaagcatgt ggtcgaggcc ccgctgctgc
      121 gcgcggtcga gctcgatctc gcggcggaca ccgcgcggaa tcgcgcgctg cacgaccgga
      181 tccacctggc gttcgcggtc ggccagcggt gggccaacct ggaccgcttc gtcgagtgga
      241 tctacgccga gctgttcgag atgccgctcg gcgacgcggc gctcggactc gacgtgcccg
      301 atccgtttcc gctcaccgcg gcgggatgag cccgctgact caccgctcgc gcggcaggct
      361 gaccgtcatc tgggtgcctg ttccgggctc gctggcggcg tcgatgtggc cgccgtgctc
      421 ggcgacgatc gcgtggcagg tcgccaggcc caggccggtg cccttgctct tggtggtgaa
      481 gaacgggtcg aagatccggg cgagctggtc ggcggggatc ccggggccgt cgtcggacac
      541 caccagcgtg gtggcgtcga ggccgcgccg cgcgtcgacg tggacgtggc ggccgcccag
      601 cgccgcggcg tcggccgcgt tgcgcaagag gttccacagc acctggcgca gcttggcggg
      661 gtcggcgtgg agcggcagcg gccggtcgac cgcgaacgcc atctcgacgc tggcgaacgc
      721 ctgctcgcgg cgcgcgacct gcagggtctc ctcgaccatc acgccgaggt cgaagtcgac
      781 cggctggggc ggccgcgggt tggcgtagtc gagcaggtcg ccgatcagca cgttgagccg
      841 ctggacctcg cggtggacga tcgccatcag ggtgcggtcg tcgtcggacg ccgacgggcc
      901 ctggcgcaac agctcgatcg agcccgagat cgacgccagg ggattgcgga tctcgtgggc
      961 gatgccggcg gcgagctggc cgatcatcgc gagccgctcg gaccggcgcg actgggcctc
     1021 gagccggcgc agctcggtga ggtcctggaa gttgacgacc cggccgagca cctggttctt
     1081 gacgtcgcgc agcggcgaca cggtgacgcc gatggtgaga tcggcggcga gctggaggtc
     1141 ggcgcgccgc agctcgccgt ggccggtcgc gatcagcgcc gacagcccgg gcatcagctg
     1201 gtcgaccggc tggccggcaa gcgcgggcgg ctggccgagg atgtcggcgg cggcgctgtt
     1261 ggcggtcagg atgatgccgt cgggcgcgat cgtgatcagc cccgaggcca gcgagcggac
     1321 gatgtcctcg tgcacggtga ggaggtcggc cgccgccttg cgggtggtcg ccagggtctc
     1381 gacgccgcgc tgcagctggt cgccgaagat gaacgacagc gcgccgaccc cgatcagcgc
     1441 cgcgaggttg atgccgagcg tgcggaccag gtcgacgccg ctctgctccc acggccggac
     1501 ctgggacagc gcgggcagcg cgatcgcctg ggtccaggcg agcagcgaca ccacgatcag
     1561 cagactgatc gacgcgagcg tgatcaccac cgcgccgccg cggtacgaca gcgcgccggc
     1621 gccgacgacc gacagcgcag agaggaagat gtacgggctc tcggcgccgc cggtcgcgta
     1681 gatcaggagc gaggccaccg cgaggtcggc ggcgtgcatc ggccgcgcga cctggcgagg
     1741 cgagaagccg cggcgcacca tcacgccgaa cacgatcgac gtcgcgtagg tcgcggcgat
     1801 gatcccggac tgcagccaca ccgccgactc cggcacctgc tcggcggtgg tcaggatcca
     1861 cagcgacagc ccgagcaccg cggtcacgac cagcgtgcgc agcagcagca gccggatcac
     1921 gcggcgcggg gtgtcgctgg ccggcggcgc cggcgactgg atcagcgagt cggcgggcga
     1981 cggaccgagc cgctcgcggc tgcgcgtccg cggccggatc ggcagcacgg gctcgctctt
     2041 gcggtgcgcc accggctagc cggtcccgat gttgccggcg agcttgaaga tcggcaggta
     2101 catcgcgatg atcaggccgc cgaccacgac gccgaggaac gccatcatca ccggctcgat
     2161 cagcgaggtc agcgccgaca ccgcggcgtc gacctcctcc tcgtag
//
LOCUS       DASVOR010000965         3433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447800,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000965 DASVOR010000000
VERSION     DASVOR010000965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447800"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VF516_18650"
     CDS             <1..455
                     /locus_tag="VF516_18650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109762.1"
                     /translation="RIVDPVEVQKLPELGVLSAMANPDLEVVSVAMSVVSRLPEDRAR
                     LYWDVILAVLPPPLREALNMETEAQRYARMRAELARMMAQQEREEGLRRGVLALVRAK
                     LDAVSREDEAAIEALDDEKALIDLIGELGRATSAAEIRGAVAAARTPR"
     gene            complement(492..1709)
                     /locus_tag="VF516_18655"
     CDS             complement(492..1709)
                     /locus_tag="VF516_18655"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109763.1"
                     /translation="MHIVFIEPRFPGNQKLFVQALAEIGATVTAIGEGSKASLDDDLK
                     HWLTHYEEVGNVTDEGAVLGALRFIQSRRHVDRLEATVEAHILPIARVREAAGIPGTS
                     VRTAFLCRDKPSMKEVLRDGGVPCAQSTAASTAAEVHAFAEQVGFPLILKPRDGAGAS
                     GATRVDSAAEIDAALGSFGAARSIAVEEFIEGHEGFYDTLTVGGAVAHDFATHYYPNV
                     LEAMRTRWISPQFIATNRIDGAPAYTELRQMGAKVIQLLGLETSATHMEWFFGPKGLK
                     FSEIGCRPPGVRAWDLYNVGNDMNLYREWALAVCTGHTQATASRRYSAGIIALRPDRD
                     GHISGYEGLEAMGNAFGQHLIDVHLPPNGTPTQPVAAGYMANAWLRMKHENYDELRRI
                     LDIVGQTVKVHAR"
     gene            complement(1713..3158)
                     /locus_tag="VF516_18660"
     CDS             complement(1713..3158)
                     /locus_tag="VF516_18660"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEX8109764.1"
                     /translation="MNILYVTSEVARYAKTGGLADVSAALPAYLGRAGHDVRVFLPYY
                     SRVDERGATLEAVLRDIEIRLGNHHYEVAIVRASRPGDAPVHLVHCPALYHRPSLYTS
                     DLDEHLRFLVLIRAALEACGRMQFAPDVIHANDWQAALAPLTLKVRYSWDRLFDRTRT
                     LLAIHNLHYQGTFRADTLADTGLADAAHLFHQDQLREGRLNFLLHGILYADGIVTVSP
                     TYAREIQGDELGAGLDPFLRSRSSTVVGILNGVDYAEWSPDRDPLIPARYTAADRAGK
                     AICKAALVERFGLPHAADVPVIGIVSRLAGQKGFALVGDVIPGLLHRHGFQLVVLGSG
                     EPRFEQLFAHLAANFPRQVGFHRGFSNELAHLIEAGADMFLMPSRYEPCGLNQMYSLR
                     YGTVPIVHRTGGLADTVRLWDPRDRTGTGLVFDHFTAEGLSWAVESALATYRDPAAWR
                     ALTDNGMAENFSWDVQGKLYEELYRRLTASR"
BASE COUNT          515 a         1125 c         1297 g          496 t
ORIGIN      
        1 cgcgcattgt cgatccggtg gaggtccaga agctgccgga gctcggagtc ctgtcggcga
       61 tggccaaccc ggatctcgag gttgtgagcg tggcgatgtc tgtcgtttcc cggctgccgg
      121 aggatcgagc ccggctatac tgggatgtga tcctggcggt gcttccgccg ccgcttcgag
      181 aggcgctgaa catggaaaca gaagctcagc ggtacgcgcg catgagggcc gaactggcgc
      241 gcatgatggc ccagcaggag cgcgaggaag gcctgcgccg cggtgtgctc gccttggtgc
      301 gcgcgaagct cgacgccgtg tcccgagaag acgaggctgc gatcgaggcg ctcgatgacg
      361 agaaggcgct gatcgacctg atcggcgagc tcggccgcgc gaccagcgct gccgagatcc
      421 gcggcgcagt ggcggccgcg cgcacgccgc gttgagggcg accggcggcg agcccgtccg
      481 gccggatgcg ctcagcgggc gtggaccttg acggtctggc cgacgatgtc gaggatgcgg
      541 cgcagctcgt cgtagttctc gtgcttcatg cgcagccacg cgttggccat gtagcccgcg
      601 gcgaccggct gggtcggcgt gccgttgggc ggcaggtgga cgtcgatcag gtgctggccg
      661 aacgcgttgc ccatcgcctc gaggccctcg tagcccgaga tgtggccgtc gcgatcgggg
      721 cgcagcgcga tgatgccggc ggagtagcgc cgcgacgcgg tcgcctgggt gtgcccggtg
      781 cacaccgcca gcgcccactc gcggtagagg ttcatgtcgt tgccgacgtt gtagaggtcc
      841 caggcgcgca cacccggcgg ccggcagccg atctcgctga acttgaggcc cttggggccg
      901 aagaaccact ccatgtgggt cgccgaggtc tcgaggccga gcagctggat gaccttggcg
      961 cccatctggc gcagctcggt gtaggccggc gcgccgtcga tccggttggt cgcgatgaac
     1021 tgcggcgaga tccaccgggt gcgcatcgcc tcgaggacgt tggggtagta gtgcgtggcg
     1081 aagtcgtggg cgacggcgcc gccgacggtc agcgtgtcgt agaacccctc gtggccctcg
     1141 atgaactcct cgaccgcgat gctacgtgcg gcgccgaacg agcccagcgc ggcgtcgatc
     1201 tcggcggcgg agtcgacccg ggtcgcgccc gacgcgccgg cgccgtcgcg cggcttcagg
     1261 atcagcggga agccgacctg ctcggcgaac gcatggacct cggcggcggt cgacgcggcg
     1321 gtcgactggg cgcacgggac gccgccgtcg cgcagcacct ccttcatcga aggcttgtcg
     1381 cggcacagga acgcggtgcg caccgacgtg ccggggatgc cggcggcctc gcggacccgg
     1441 gcgatcggca ggatgtgggc ctcgaccgtg gcctcgagcc ggtcgacgtg cctcctggac
     1501 tggatgaagc gcagcgcgcc gagcaccgcg ccctcgtcgg tcacgttgcc gacctcctcg
     1561 tagtgggtca gccagtgctt gagatcgtcg tcgagcgacg ccttgctgcc ctcgccgatc
     1621 gcggtcacgg tggcgccgat ctcggcgagc gcctggacga acagcttctg gttgcccgga
     1681 aaacgcggtt cgatgaacac gatatgcacg ggtcacctcg atgcggttag gcgtcggtac
     1741 agctcctcgt acagcttgcc ctggacgtcc caggagaagt tctcggccat gccgttgtcg
     1801 gtcagcgcgc gccacgccgc ggggtcgcga taggtcgcga gcgcggactc gaccgcccag
     1861 ctcagaccct cggcggtgaa gtggtcgaac accaggccgg tgccggtgcg atcgcgcgga
     1921 tcccacagcc ggacggtgtc ggcgaggcct ccggtccggt ggacgatcgg cacggtgccg
     1981 tagcgcaggc tgtacatctg gttgagcccg cacggctcgt agcgcgacgg catcaggaac
     2041 atgtcggcgc cggcctcgat caggtgggcg agctcgttgg agaacccgcg gtggaacccg
     2101 acctggcgcg ggaagttcgc ggcgaggtgg gcgaacagct gctcgaaccg cggctcgccg
     2161 ctgccgagca cgacgagctg gaagccgtgg cggtggagca gccccgggat gacgtcgccg
     2221 accagcgcga agcccttctg gccggcgagc cgcgacacga tgccgatcac cgggacgtcc
     2281 gcggcgtgcg gcagcccgaa ccgctcgacc agcgccgcct tgcagatcgc cttgccggcg
     2341 cggtcggccg cggtgtagcg cgccgggatc agcggatcgc gatcggggct ccactcggcg
     2401 tagtcgacgc cgttgaggat gccgaccacg gtcgacgacc gcgagcgcag gaacggatcg
     2461 agcccggcgc cgagctcgtc gccctggatc tcgcgcgcgt aggtcgggct gaccgtgacg
     2521 atgccgtcgg cgtacaggat gccgtggagc aggaagttga gccggccctc gcgcagctgg
     2581 tcctggtgga acaggtgcgc ggcatcggcc aggccggtgt cggcgagggt gtcggcgcgg
     2641 aacgtgccct ggtagtgcag gttgtggatc gcgagcagcg tccgggtccg atcgaacagc
     2701 cgatcccacg agtagcggac cttgagcgtg agcggcgcga gcgcggcctg ccagtcgttg
     2761 gcgtggatga cgtcgggcgc gaactgcatc cggccgcacg cctcgagcgc cgcgcggatc
     2821 agcaccagga accggaggtg ctcgtcgaga tcgctggtgt acagcgacgg gcggtggtag
     2881 agcgccgggc agtgcaccag gtggaccggc gcatcgcccg gccgcgacgc gcgcacgatc
     2941 gcgacctcgt agtggtggtt gccgagccgg atctcgatgt cgcgcagcac cgcctcgagc
     3001 gtcgcgccgc gctcgtcgac ccggctgtag tacggcagga acacccgcac gtcgtggccg
     3061 gcgcggccga ggtacgcggg cagcgcggcc gacacgtctg cgaggcctcc cgtcttggcg
     3121 tagcgcgcga cctcggacgt gacgtacaga atgttcacgt attccgcatc atggcacgga
     3181 agcccgcggc ggtgggcggc cacgctcccg accggcgcgg catgcgtcgt gcactcctcg
     3241 cacgacacgg aggagatcat gccagagcga ggcacggcac aacgagccaa gcagaagctg
     3301 cgcagcgggc gaagcgcgtc gaccgcggcc ggcgagttcg tccgcgagga gatggaacac
     3361 atccgggagg gcaagcacgg cgcgcgctcg accaagcagg cgatcgcgat cggcctgtcg
     3421 aaggctcgtc gcg
//
LOCUS       DASVOR010000966         4662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_126493,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000966 DASVOR010000000
VERSION     DASVOR010000966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126493"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="VF516_18665"
     CDS             complement(<1..383)
                     /locus_tag="VF516_18665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109765.1"
                     /translation="MNEDGSAEGGRTRLARGTKPHALTGPQELAGAEPLENAPGPVVS
                     HARSDAPVPPVSTPAEARRSVLSASVDTLSRAPVTSRATDAIHGGPVQAGADATVGGA
                     VSAGLDARGSGPVPAGGAPSGAGRAP"
     gene            complement(445..1077)
                     /locus_tag="VF516_18670"
     CDS             complement(445..1077)
                     /locus_tag="VF516_18670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8109766.1"
                     /translation="MAELERWIEITSQAELSELLGVPSERAANKDRVRLHERDREWLA
                     ASPFCLVATANADGSCDVSPKGDPPGFTLVLDDATIAIPERSGNRRADGFRNVLANPH
                     VGLIYLVPGRGDTLRINGRARVVRDAAFFDRMIVKGHRPQLALVVEIEQIFFHCAKAF
                     LRSSLWQPETWRPAALPSRAALCKAVEPTAESLEDLEQYYGPEYARLLYG"
     gene            complement(1390..3003)
                     /locus_tag="VF516_18675"
     CDS             complement(1390..3003)
                     /locus_tag="VF516_18675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8109767.1"
                     /translation="MMAFDLVFMDVARRESRAIQTIDQRGRPATFLFREFYCADPGCD
                     CRRVVLHVHWVEEQCIAASINYAFEPSRRRDEPQISLDPLNPQSEHAEELLGLFTEAI
                     RKDREFRERLLRHYTAWKQVVDDASHPQHAKVRGAAHGDPSFRPAFPRKKAQTRGRRA
                     AAAAGADGSQSLQRVAAKAGAGDSKLQRQFRRVLQKVDRLKQQVRGWKEARADIDTEI
                     SRYAASLEQLRGLGRAMVGLLDRAYPDAVFSKADRKKLGELICEIAGDLIDEGSHDDL
                     KPLYNRYSRSDFDAEAAAADAQSAAALRSMMEMFGLEFGDADVGSLDKLKAFTEDQMR
                     EAEEEAAAEDERRARRKKSAKQQAAEARREAEQRSTGKALQDVYRALAKALHPDREQD
                     PVERARKTEVMREVNVAYEAKDLLRLLELQLELERMEAAEADAIAEERLRHYIRILEE
                     QARQLAVELEELELPFRLELGLEPSARLAPAAVISRVRADAAEVKEQIARMEHDLAAF
                     QTAGRLKAWLKTQPRRSRRDRGAGIDLAG"
     gene            3296..3370
                     /locus_tag="VF516_18680"
     tRNA            3296..3370
                     /locus_tag="VF516_18680"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3328..3330,aa:Gly,seq:ccc)
     gene            complement(3374..>4662)
                     /locus_tag="VF516_18685"
     CDS             complement(3374..>4662)
                     /locus_tag="VF516_18685"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8109768.1"
                     /translation="LRRVGTRTMWMRQQGQKTRYPGVYRVNDNSYRVRAVGTDPRTGK
                     KKAVEKLYEGVSAQQAARLRTDLIEEIVAATRVATKQRVSDFARSWIASKAVSVDAAT
                     ASTYADALEQHVLPVLGDYWYDALTKADVQKWIDDCFSAVWKTPSGKKKRYSRNSVHG
                     WFRVFRTMTRDAVDALHLLRDPCVRIRFPQEELCEESNALDPEELLRFLAEMRARYPH
                     HHAMVTLLAFTGLRFCHASALKWEDWDEDKAVLHVRRKHVRGDVAPVSRKKRAPGVIP
                     VEPELAEVLREHRARLVAAKAPGFAQGWMFPSDAGTLRTPNTMDRAWAKCLDAAKVKH
                     RFTVHGLRYTFTDLIRLSKADAVVRRALTGHVTQEMQQHYSNVGTEEKRAAIAGALRL
                     LADTKVAAQNQPPPAHEPAGVNLGVNRGGSEGVSVR"
BASE COUNT          625 a         1868 c         1411 g          758 t
ORIGIN      
        1 ggcgcgcggc cggcaccgct tggtgcacca cccgccggca cgggcccgct gcccctggcg
       61 tccaggcccg cggacaccgc accgccgacg gtggcgtccg cacctgcttg caccggcccg
      121 ccgtggatcg cgtccgtggc cctgcttgtc accggcgcac ggctcagggt gtccacgctc
      181 gccgacagga cgctgcggcg cgcttccgcc ggcgtgctca cgggtggcac cggcgcatcg
      241 ctgcgggcat gcgacacgac cggtcccggc gcgttctcca ggggctccgc gcccgcgagc
      301 tcctgcgggc cggtcagcgc atgcggcttc gtcccgcgcg ccaggcgcgt acgcccgccc
      361 tcggcggagc cgtcctcgtt catgcccaca ccatcgccga tcggcctgac atcgcgaagg
      421 catcggccga cgctgcgcag ctgctcatcc gtacagcagc cgggcgtact ccgggccgta
      481 atactgctcc agatcttcca gcgactccgc cgtgggctcc accgccttgc acagcgccgc
      541 gcgcgacggc agcgcggcgg gccgccaggt ctccggctgc cacagcgacg atcgcaggaa
      601 cgccttcgcg cagtgaaaga agatctgctc gatctccacc accagcgcca gctgcgggcg
      661 gtgccccttc acgatcatcc ggtcgaagaa cgccgcgtcc cgcaccaccc gggcccgccc
      721 gttgatccgc agcgtgtcgc cgcggcccgg caccaggtag atcagcccga cgtgcgggtt
      781 cgccagcacg ttgcggaacc catccgcccg ccggttgccc gagcgctccg ggatcgcgat
      841 cgtcgcgtca tcgagcacca gcgtgaaccc cggcgggtcc cccttgggcg atacatcgca
      901 gctgccgtcc gcgttcgccg tcgccaccag acagaacggc gacgccgcca gccactcgcg
      961 gtcgcgctcg tgcagccgca cccgatcctt gttcgccgcg cgctcgctcg gtactcccag
     1021 cagctcgctc agctccgcct gcgacgtgat ctcgatccag cgctccagct cggccatgcg
     1081 ttcagcgtag cgccgccggc ccgcggttgg ccaccggcga accgccgccc gccggcgcag
     1141 ccgccatgcc gatcaccgat acagcggctc gaggccaccc gccgcgcgcg ctgccacccc
     1201 gcgtcgatcg gtagctcgcg aggccgcggg gagccggccg accgaccccg ggctgatccg
     1261 gccgctgacc ggcgatgact gcttcggcgt ggtcatgccg atgaggatct agagccctgc
     1321 aacctcgcac tggtcgccac gggcgtgcgc aacctgcggc cgctccagcg ctgctgcgcg
     1381 ccgggccgtt cacccggcca gatcgatccc ggccccgcgg tctcgccggc tgcgccgcgg
     1441 ctgcgtcttc agccacgcct tcagccggcc cgcggtctgg aacgccgcca ggtcgtgctc
     1501 catcctcgcg atctgctcct tcacctccgc ggcatcggcg cgcacccgcg agatcaccgc
     1561 ggccggtgcg agccgcgcgg acggctccag ccccagctcc agccggaacg gcagctccag
     1621 ctcctccagc tcgaccgcga gctgcctggc ctgctcctcc aggatccgga tgtagtggcg
     1681 cagccgctcc tcggcgatcg cgtccgcctc tgccgcttcc atgcgctcca gctccagctg
     1741 gagctccagc agccgcagca ggtccttcgc ctcgtacgcc acgttgacct ctcgcatcac
     1801 ctcggtcttg cgcgctcgct ccaccggatc ctgctcgcgg tcgggatgca gcgccttcgc
     1861 cagcgcgcga tagacgtcct gcagcgcctt gcccgtgctc cgctgctccg cctcgcgccg
     1921 cgcctccgcc gcctgctgct tcgccgactt cttgcgccgc gcccgccgct cgtcctcggc
     1981 cgcggcctcc tcctccgcct cacgcatctg gtcctcggtg aacgccttca gcttgtcgag
     2041 cgacccgacg tccgcgtcgc cgaactccag gccgaacatc tccatcatcg accgcagcgc
     2101 cgccgcgctc tgcgcatcgg ccgccgccgc ctcggcatcg aagtcactgc ggctgtagcg
     2161 gttgtacagc ggcttcagat catcgtggct gccctcgtcg atcaggtccc cggcgatctc
     2221 gcagatcagc tcgcccagct tcttgcgatc cgccttgctg aacactgcat cgggatacgc
     2281 ccgatccagc aagccgacca tcgcgcgccc gagcccgcgc agctgctcca gcgacgccgc
     2341 gtaccgcgag atctccgtgt cgatatccgc ccgcgcctcc ttccaccccc ggacctgctg
     2401 cttcagccgg tcgaccttct gcagcacgcg gcggaactgc cgctgcagct tgctgtcccc
     2461 ggcgccggcc ttcgccgcca cgcgctgcag gctctgcgac ccgtccgctc cggcggcggc
     2521 cgcggcgcgc cgcccccgag tctgcgcctt cttccgcgga aacgccgggc gaaacgacgg
     2581 atcgccgtgc gccgcaccgc gcaccttcgc gtgctggggg tgcgacgcat cgtccaccac
     2641 ctgcttccac gccgtgtaat ggcggagcag ccgctcgcga aactcacgat ccttgcggat
     2701 cgcctcggtg aacagcccca gcaactcctc ggcgtgctcg ctctgcggat tcaacgggtc
     2761 gagcgagatc tgcggctcgt cccgccgccg cgacggctcg aacgcgtagt tgatcgacgc
     2821 cgcgatgcat tgctcctcga cccagtgcac gtgcagcacg acccgccggc agtcgcaccc
     2881 cggatcggcg cagtagaact cgcggaacag gaacgtcgcc ggcctccccc gctggtcgat
     2941 cgtctggatc gcccggctct cgcgccgcgc gacgtccatg aacacgaggt cgaacgccat
     3001 catcgccatc ggcatctccg ccgggcgacc agccggacca ggctccgcga gctcatggcc
     3061 cggaccctgc cacgccggcc tgacaccgca tacgtcccgc gccagtgctc gacgttcagc
     3121 cctcgctgca gcggcgcgta cgcctctccc tcgacctcga gcccgagacg cctgaagact
     3181 tacgagtact tgaggcttcg cgctgccttg acgtttgctc ggcgatccaa ccgcaacgtc
     3241 cgcgccgttc cgagccttga cggcccgctg ctttcgtgta tgtttacgag cacctgcggg
     3301 attaactcag cggtagagtg tcagcttccc aagctgaagg tcgccggttc gaatccggta
     3361 tcccgctcca tacttagcga accgacaccc cctcggaacc ccctcggttc acacccaagt
     3421 tcacaccagc gggttcgtgc gcaggcggcg gctggttctg cgccgcaacc ttggtgtccg
     3481 ccagaagtcg aagcgccccg gcgatggcgg cccgcttctc ttcggtcccg acgttcgagt
     3541 agtgctgctg catctcctgc gtcacgtgcc ctgtcagcgc ccgccggacc accgcgtccg
     3601 ccttcgacag ccggatcagg tccgtgaacg tgtaccgcag gccgtgcacg gtgaagcggt
     3661 gcttcacctt cgcggcatcg aggcacttcg cccacgcccg atccatcgtg tttggcgtcc
     3721 gtagcgttcc ggcatcggac gggaacatcc agccctgagc gaaccccggc gccttcgcgg
     3781 ccaccaggcg cgcccggtgc tcgcgaagca cctccgccag ctccggctcg accgggatca
     3841 cccccggtgc ccgcttcttg cgtgacaccg gcgcgacatc gccccgcaca tgcttccgcc
     3901 gcacgtgcag caccgccttg tcctcgtccc agtcctccca cttcagcgcc gacgcatggc
     3961 agaaccgcag ccccgtgaag gcgagcagcg tcaccatcgc gtggtgatgc gggtaccgcg
     4021 cgcgcatctc ggcgaggaat cgcaacagct cctcgggatc gagcgcgttc gactcctcgc
     4081 acagctcctc ctgcgggaac cggatccgta cgcacggatc ccgcagcagg tgcagggcgt
     4141 ccaccgcatc gcgggtcatg gtgcggaaca cccggaacca gccgtggacc gagttccgcg
     4201 agtagcgctt cttcttcccg ctcggcgtct tccagaccgc gctgaagcag tcgtcgatcc
     4261 acttctgcac gtccgccttg gtcaaggcgt cgtaccagta gtcgccgagc accggcagca
     4321 cgtgctgctc gagcgcgtcc gcgtacgtgc tagccgtcgc cgcgtcgacg ctgacggcct
     4381 ttgatgcgat ccacgatctc gcgaagtccg aaacgcgctg cttggtcgcg acgcgggttg
     4441 ccgcgacgat ctcctcgatc aggtccgtcc gcagccgcgc ggcctgctgg gcgctcaccc
     4501 cttcgtagag cttctccacc gctttcttct tgccggtgcg cgggtcggtc ccgacggcgc
     4561 gcacgcggta gctgttgtcg ttcactcgat acacgcctgg gtaacgagtc ttctgccctt
     4621 gctggcgcat ccacatcgtc cttgtcccca cgcgtctcaa gg
//
LOCUS       DASVOR010000967         5310 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_185285,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000967 DASVOR010000000
VERSION     DASVOR010000967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185285"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /locus_tag="VF516_18690"
     CDS             <1..668
                     /locus_tag="VF516_18690"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX8109769.1"
                     /translation="APDRAAPKPEAARPEPPKADPKDDLKIVDKPIPFDDERVRLTLA
                     YRRQHSDPGAKDIAIEPRVIVLHYTAGGTAKGTFEYFSGNRMEAGRPQLAAAGDVNVS
                     SHFLVDRDGTIYRLMPETRFTRHCIGLNHIAIGIENVGDEHRWPLTDAQVEADARLVR
                     HLAAQFPITHLVGHSEAARLEGKIYFQERDPRYRDSKPDPGDRFMKLVRAKLTDLKLQ
                     GPP"
     gene            complement(857..1297)
                     /locus_tag="VF516_18695"
     CDS             complement(857..1297)
                     /locus_tag="VF516_18695"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX8109770.1"
                     /translation="MSAPARSRIAALGHAALAGVLFGAGLVIAGMTQPARIIAFLDPL
                     GGWDPSLACVMAGAVAVYALAYAVIRRRARPWFDAQFHVPVRADIDGPLVIGAAVFGI
                     GWGLGGLCPGPGIVAAAGGSTSALAFVLAMLAGMHLRHRLAALR"
     gene            complement(1294..1707)
                     /locus_tag="VF516_18700"
     CDS             complement(1294..1707)
                     /locus_tag="VF516_18700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX8109771.1"
                     /translation="MGSNWGLALLGGALIGSGSALLLLTHGRIAGISGIAGGLVQRST
                     PDRAWRLAFLAGLVAAGAVTASIAPAAIGAPVRSLAAMTVAGLLVGFGTRLGSGCTSG
                     HGVCGLSRLSVRSLIAVATFMTTGAITAMIAGRLW"
     gene            1893..2678
                     /locus_tag="VF516_18705"
     CDS             1893..2678
                     /locus_tag="VF516_18705"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8109772.1"
                     /translation="MLIIHASDFTGDDAAASLHAAALASRGARLITLHTGPARPVGHD
                     EHEPSPGATELAARWGRTIDHELVQIASLADEISDGIADSLIEALRELRPELIVVGTH
                     ARRGIAAWIRSSISEAIARNVAVPVLVVPNRARGFVDPRDGTVDLRRIVVPADHAEAA
                     RRGAEAARWLAQLAGHAARAEDELVIVHAGAPDPDLHRLGIPVLGIEGVLEDAIAEAA
                     RGRDACLIAMATRGHDGIGDVFRGSHTERVIREASCPVLSVPI"
     gene            2765..3511
                     /locus_tag="VF516_18710"
     CDS             2765..3511
                     /locus_tag="VF516_18710"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8109773.1"
                     /translation="MFALGLILSLAIGLSLGLLGGGGSILTVPVLHYALGLGVHEAIA
                     GSLVVVAVTSSIAVIPYARSGRVQWRIGLVFGAASMAAAFAGGRLGARLPGGVLIAAF
                     ALVMIAAGAAMLLRSRRALGLPVGRPVRTPRVLAVGLGVGLVTGVLGAGGGFVIVPAL
                     TLLGGLAMCDAVGTSLLVIAMNALAGLAGGALHTHVDVRTIAAVTGLAVIGSLAGARV
                     GRRVSAHRLQQGFGGFVLAVAAAILLGELV"
     gene            3616..4545
                     /locus_tag="VF516_18715"
     CDS             3616..4545
                     /locus_tag="VF516_18715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8109774.1"
                     /translation="MQIQAFFDPDTFTLTYAVFDAASGDAVVIDPVLDYDVLVSRTST
                     RSVERVAAFLRERELRLHDVLETHAHADHLSGSQWLRQHFGARIAIGERIREVQDTFR
                     AALDLPHLATDGRQFDRLLADGDVIAAGTLAIHTIATPGHTPACVTYQIGDALFTGDA
                     LFLHDYGTGRCDFPRGSAEALYDSIQRLYALPDATRVFPGHDYQPGGRAVAWETTIGR
                     SKRENPQLRATTTRAEFVALRTARDRTLAPPRLLYPSVQVNIDAGRLPPPHANGGRYL
                     TIPISEAPERQVEHLDHAIPRLDQRDTTSPRAT"
     gene            complement(4707..5147)
                     /locus_tag="VF516_18720"
     CDS             complement(4707..5147)
                     /locus_tag="VF516_18720"
                     /inference="COORDINATES: protein motif:HMM:NF042761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6691 family protein"
                     /protein_id="HEX8109775.1"
                     /translation="MSAPARSRGAALGVAVIAGMLFGAGLVISGMTQPARIVAFLDPF
                     DGWDPSLAFVMAGAVAVYAVAYAVIRRHARPWFDARFHVPVRADLDARLVVGAAVFGI
                     GWGLGGFCPGPSIVAAAGGSTSAIVFVLAMLAGMYVRHRRAAPR"
     gene            complement(5144..5299)
                     /locus_tag="VF516_18725"
     CDS             complement(5144..5299)
                     /locus_tag="VF516_18725"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HEX8109776.1"
                     /translation="MLVGFGARLGDGCTSGHGVCGLSRGSGRSLVAVATFMATGAITA
                     IVAGRLS"
BASE COUNT          802 a         1981 c         1837 g          690 t
ORIGIN      
        1 ccgcgccgga tcgcgccgcc ccgaagcccg aagccgcccg gccggaaccg ccgaaggccg
       61 acccgaaaga cgatctgaag atcgtcgaca agccgatccc gttcgatgac gagcgcgtcc
      121 ggctcacgct cgcgtaccgc cgccagcaca gcgatccggg tgccaaggac attgcgatcg
      181 agcccagggt gatcgtcctg cactacaccg cgggcggcac cgccaagggc accttcgaat
      241 actttagcgg aaaccgcatg gaggccgggc gcccccagct cgccgccgcc ggcgacgtca
      301 acgtctcgtc ccacttcctg gtcgaccgcg acggcacgat ctaccgcctg atgcccgaga
      361 cccggttcac gcgccactgc atcggcctca accacatcgc gatcgggatc gagaacgtcg
      421 gcgacgagca caggtggccg ctgaccgacg cgcaggtcga ggccgacgcg cgcctggtcc
      481 gccacctcgc cgcgcagttc ccgatcaccc acctggtcgg ccacagcgag gccgcgcgcc
      541 tggagggcaa gatctacttc caggagcgcg acccccgcta ccgcgactcg aagcccgatc
      601 cgggagatcg cttcatgaag ctcgtgcgcg ccaagctcac cgatctgaag ctgcaaggcc
      661 caccgtgatc gccgtgaccc ggcgcgcggc gctcgcggcc ctcgccctcg gcgaggcaca
      721 gcaccggccc cgcggggatg cgcgccgcct cgtcgcgcag aaacccgttc ggctcgacgc
      781 catacgcata gtccgggcca gcataccgct cgtcccacat ctctctcttc atgcgatgtt
      841 ccatcttacc tcccgttcac cgcagggcgg caaggcgatg ccgcaggtgc atgccggcga
      901 gcatcgcgag cacgaaggcg agcgcgctgg tgctaccgcc ggcggcagcc acgatgccgg
      961 ggcccggaca caggccgccg agcccccagc cgatgccgaa cacggccgcg ccgatgacga
     1021 gtggcccgtc gatgtcggcc cggaccggca catggaactg cgcatcgaac cacggccgcg
     1081 cgcgccggcg gatcacggcg tatgcgaggg cgtagaccgc gaccgcgccg gccatcacgc
     1141 acgccaggct cgggtcccag ccgccgagcg ggtcgaggaa ggcgatgatg cgcgccggct
     1201 gggtcatgcc cgcgatcacc agcccggcgc cgaacagcac ccccgcgagc gccgcatggc
     1261 ccagcgcggc gatccggctc cgcgccggcg cgctcaccac agcctcccgg cgatcatcgc
     1321 ggtgatcgcg ccggtggtca tgaacgtggc gacggcgatc agcgagcgga ccgagagccg
     1381 ggacagcccg cacaccccgt ggccgctggt gcagccgctg cccagccgcg tgccgaaccc
     1441 gacgagcagc ccggcgacgg tcatcgccgc cagcgatcgc acgggcgcgc cgatcgccgc
     1501 gggcgcgatg gacgcggtca cggcacctgc ggccacgagc ccggcgagga acgcgagccg
     1561 ccaggcgcga tcgggcgtgg aacgctggac gaggccaccg gcgatcccgc tgatgccagc
     1621 gatccgcccg tgggtgagca ggagcagcgc gctgcccgag ccaatcagcg caccgccgag
     1681 caaggcgagt ccccagttcg aacccatgtg aggaacgata agcactccgg gtgccagcca
     1741 cgcaacccgg gccacgcggg attgccaccg tctgcgagcc tgcggccccg gtacgtcgtt
     1801 gcacgggcgt ttcagcgagt ttcatggcgg cgacccgcga agcggctccg gatcggcccg
     1861 atgtggcatc gcgcttgaac gactcgcgtt cgatgctgat cattcacgcc tccgacttca
     1921 ccggcgacga cgccgcggcc tccctccacg cggcggcgct ggcgagccgc ggcgcgcggt
     1981 tgatcacact acatacaggg cctgcccggc cggtgggaca cgacgagcac gagccgtcgc
     2041 cgggtgcaac cgagctcgcc gcgcgctggg ggcggacgat cgatcacgag ctcgtgcaga
     2101 tcgcgagcct cgccgacgag atctcggacg gcatcgccga cagcctgatc gaggcgctgc
     2161 gcgagctccg gcccgagctc atcgtggtag ggacccacgc gcggcgcggg atcgccgcct
     2221 ggatccgcag cagcatcagc gaggcgatcg cgcgcaacgt cgcggtgccc gtgctggtgg
     2281 tacccaaccg cgctcgcggg ttcgtcgatc cgcgcgacgg gacggtcgat ctgcgccgga
     2341 tcgtcgtgcc ggcagaccat gcggaggcgg cgcggcgggg cgcagaagct gcgcgatggc
     2401 tcgcacagct cgcgggccac gcggcccggg cggaggacga gctggtgatc gtgcacgccg
     2461 gtgcgcccga ccccgatctg caccgactgg gcattccggt gctcgggatc gagggcgtgc
     2521 tcgaggatgc gatcgccgag gcggcgcggg ggcgcgacgc gtgtctgatc gcgatggcga
     2581 cccggggcca cgatggcatc ggcgacgtgt ttcgcggctc gcacaccgag cgggtgatcc
     2641 gcgaggcgag ctgtccggtg ctctcggtgc cgatctgaga cgtttcgcgc cgtttcatcg
     2701 ccgcgccgaa ccggtcgcgg tcagcgatac cgcttgcggc ccggcgcttg ctgaagcctt
     2761 ggccatgttc gcgctcggtc tgatcctgtc gctcgcgatc ggcctgtcgc tcgggctgct
     2821 cggtggtggt ggctcgatcc tcacggtgcc ggtcctgcac tacgcgctcg ggctcggcgt
     2881 gcacgaggcg atcgccgggt cgctggtcgt ggtcgcggtg accagcagca tcgccgtgat
     2941 tccgtatgcg cgcagcggtc gagtacagtg gcgcatcggc ctggtattcg gcgccgcatc
     3001 gatggcagcc gcgttcgccg ggggccggct cggcgcacgg ctgcccggcg gggtgctgat
     3061 cgcagcattt gcgctggtca tgatcgcggc gggcgcggcg atgctactgc gatcgcgccg
     3121 cgcgctcggg cttccggtcg gccggccggt ccgcacgccg cgggtgctcg cggtcggact
     3181 cggcgtcggg ctggtcaccg gcgtgctcgg tgccggcggc ggcttcgtga tcgtgccggc
     3241 gctgaccctg ctcggcgggc tcgcgatgtg cgacgcggtc ggcacctcgc tgctggtgat
     3301 cgcgatgaac gccctcgccg gcctggccgg cggcgccctg cacacgcacg tcgacgtccg
     3361 gacgatcgcc gcggtcaccg ggctcgccgt gatcgggagt ctggccggcg cgcgggtggg
     3421 acgccgcgta tccgcgcacc ggctgcagca agggtttggc ggcttcgtcc tggcggtcgc
     3481 cgccgcgatc ctgctgggcg agctggtgtg aaacgcgcgt tgcgccgcgt ttcacggcga
     3541 gcgggaagtg gcgaacgtcc agctcgccgt gatggcacct cgattgctac tcgtcgccgg
     3601 ggagggaaca tgcagatgca gatccaggca ttcttcgatc ccgacacatt cacgttgacg
     3661 tacgcggtgt tcgacgcggc gagcggcgat gcagtggtca tcgatccggt gctcgactac
     3721 gacgtcctgg tctcgcgcac ctcgacgcga tcggtcgagc gcgtcgccgc gttcctgcgg
     3781 gagcgcgagc tgcgcctgca cgacgtgctg gagactcacg cgcacgccga tcacctgtcg
     3841 ggctcgcagt ggctgaggca gcacttcggg gcccggatcg cgatcggcga gcgcatccgc
     3901 gaggtccagg acacgtttcg cgccgccctc gatctgccgc acctcgctac cgatggccgg
     3961 cagttcgatc gcctgctcgc ggatggcgac gtgatcgcgg ccggcaccct ggcgatccac
     4021 acgatcgcga ccccgggcca cacgccggcg tgcgtgacct accagatcgg cgacgcgctg
     4081 ttcaccggcg atgcgctgtt cctgcacgac tacggcaccg gccgctgcga cttcccacgc
     4141 ggcagcgccg aggcgctcta cgactcgatc cagcggctct acgcgctgcc cgacgccacg
     4201 cgcgtgttcc ccggccacga ctaccagccc ggcggccgcg cggtcgcctg ggagactacg
     4261 atcgggcgct cgaagcgcga gaatccacag ctgcgtgcga ccacgacccg ggccgagttc
     4321 gtcgcgctgc gcaccgcccg cgaccgcacc ctggcgcccc cgcgcctgct gtatcccagc
     4381 gtgcaggtca acatcgacgc cgggcggctg cccccacccc acgccaacgg cggtcgctac
     4441 ctgacgatcc cgatctccga ggcgccggag cgccaggtcg agcacctcga ccacgcgatt
     4501 ccccgcctcg accagcgaga cacaacatca ccgcgagcaa cctgacggac atgccgccag
     4561 catgaatcgc agcatctcgc tgatgcaatc aagtttcagt tgcagccacc ccgacgcggc
     4621 cgcgaccgac gcgatccggt ccatgcaggg gttcaccatc aggaaaccaa cgcacggacc
     4681 gcggcgatga ttccaccggc ccccggtcac cgcggagcag cgcggcgatg ccgcacatac
     4741 atgccggcga gcatcgcgag cacgaacacg atcgcgctgg tgctgccgcc ggcggcggcg
     4801 acgatgctcg ggccggggca gaacccgccc aggccccagc cgatgccgaa caccgccgcg
     4861 cccacgacga gccgcgcatc gagatcggcc cggaccggca cgtgaaaccg ggcatcgaac
     4921 cacggccgcg cgtgccggcg gatcacggcg tacgcaaccg cgtagaccgc gaccgcgccg
     4981 gccatcacga acgccaggct cgggtcccag ccgtcgaacg ggtcgaggaa ggcgacgatg
     5041 cgcgccggct gcgtcatgcc cgagatcacc agcccggcgc cgaacagcat ccccgcgatc
     5101 acggcgacgc ccagcgcggc gccccgactc cgcgcgggtg cgctcacgac agcctccccg
     5161 cgacgatcgc ggtgatcgcg ccggtggcca tgaatgtcgc gacggcgacc agcgagcggc
     5221 ccgacccgcg cgacagcccg cacaccccgt ggccgctcgt gcagccgtcg cccaggcgcg
     5281 cgccgaaccc gacgagcagc ccggcgaccg
//
LOCUS       DASVOR010000968         5058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_389392,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000968 DASVOR010000000
VERSION     DASVOR010000968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389392"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VF516_18730"
     CDS             complement(<1..1587)
                     /locus_tag="VF516_18730"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8109777.1"
                     /translation="MPPLAAVHHRNLVAVAEAALPAGRYLPAAGETTVQKVEQFLGEL
                     PDSLRRGVGGLLRSLDAAAWLTERRPFARLPIARRLKLLDGWRTADPIRRLLLRVLVS
                     PLKMAHFDDPALYQRLGCVYEATRGRETKPAYLRDRTHQLDGDLAVECDVVVVGTGAG
                     GAVVGRELAEAGLAVVFVEQGRYFDRSDFTGRSFEMQQKLYRRGGSTFSIGNVGIPIP
                     LGQTVGGTTTVNSGTCYRAPDRVLERWRDELGLTELGPDAMARHFERVEAVLHVERAR
                     AELLGGNGRVIARGCESLGLTRHGPLRRNAPACDGQGVCCFGCPTDAKRSTNVSYIPL
                     ALRAGAELFPGLRVTRIIVEGGRARGVVATSDAGHVLTVHARAVVVAGGAIMTPLLLG
                     AQQLGTASGQLGKNLSIHPAAGILAEFDEEILPWRGIPQGYAIEDLHDEGILFEGATL
                     PLEMMMTMTPLIGPDLVRLAESFDHVASFGFLVEDTSRGSVHELRGQPVIRYWLSERD
                     VAHIKRGLDVLAQIFFAAGAR"
     gene            complement(1714..2433)
                     /locus_tag="VF516_18735"
     CDS             complement(1714..2433)
                     /locus_tag="VF516_18735"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8109778.1"
                     /translation="MTSVKKQIVVETSQQRAFRTFTDGIDRWWSREHHIGASPLERMV
                     VEPRAGGRWYSICKDGSEVDVGKVVAWEPPHRLVLTWQITARWQYDPAFSTEIEVGFL
                     AEGPRRTRVELEHRQLERYGADADSMKKTFEADDAWAASLHAFAGASARGKFLMIYET
                     TPEGLARAREHLPAHRDRLDQFHARGTLLMAGPVMDGSGRAFGVFTSRQAAEDFIAGD
                     PFIVHQVVARWTVAEWNEVLF"
     gene            complement(2430..2735)
                     /locus_tag="VF516_18740"
     CDS             complement(2430..2735)
                     /locus_tag="VF516_18740"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8109779.1"
                     /translation="MANQLGFAALADDTRRTIFERVARRPSSVGKLAEGLDVTRPAVS
                     QHLKVLLRAGLVVQRRDGNRCIYQVDPRGVAAMRAYLDRFWDRALAAFKLAAEQEDT"
     gene            2872..>5058
                     /locus_tag="VF516_18745"
     CDS             2872..>5058
                     /locus_tag="VF516_18745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109780.1"
                     /translation="MQTHTWLIFAGLALGAGCTGLVNESSAELADPSTVSSASADGSA
                     AAAQFSPSAAIAGVWDPRFTIAGFTGPDGHAPTVYDFARDIDGSIVATGEFRTFGRTR
                     VEPLLRLRNGVWQPARARWEIPPPASGFSAVAIAPDGKLALATYDDFGPRAGQIWLDD
                     GTGLRVIGTFDGLIRRLRWYRGQLWAAGWDQMHQGASRIQGLAVWNGAAWTAPVGGAP
                     TGFAFELVEDAGELLVGGDFTSVGGVPSSGVSAWTGTAWHPMGFSRGATVYTLTRGPD
                     GQLYAGGALGDLGGGAGGIARWTGEGWVLVGGGVVNRTTPGVVTALTSHQGSLFAAGC
                     FVTAGGGEGMPGAVVSRDVVRFDGAWHSLDDGAHGTVAPWIEPLACGDEGPSSVWDVS
                     KQAMVSTGRQLLLGGSFAGIAGVESQAILANNGAGWLAQGDTRGIGIGGSLDRLGVAA
                     LTGQLWAAGQFSQVAGMATRGHVVRFTGDGWVPIGDAIPRDASCLAFAVAPAGEVALG
                     CTTFPSQGNAVGQVFRVQGDRLVQVGGDLPPIQAVTYDPWGRLWVAGGGGTGFVARLD
                     GEAFTLIESRFDAPVSQIDIAGSNDVIAAGAFSMVAGLPVAHIARWNGATWRPLGSGV
                     PGAVTALAHGGGIVYVSSVNDGSGALLLGAFDGITWHELATPGAGLTPQPVFNFNALQ
                     PIAGGVIAVGTAQLDNGSGRGALVYRGGRFTALGGGVHAIGLSGLAV"
BASE COUNT          640 a         1741 c         1989 g          688 t
ORIGIN      
        1 gcgggcgccg gcggcgaaga agatctgggc gagcacgtcg aggccgcgct tgatgtgcgc
       61 gacgtcgcgc tcggacagcc agtagcggat cacgggctgg ccgcgcagct cgtggaccga
      121 gccgcgcgag gtgtcctcga cgaggaaccc gaagctcgcg acgtgatcga agctctcggc
      181 gagccggacc aggtccggtc cgatcagcgg ggtcatcgtc atcatcatct cgagcgggag
      241 ggtggcgccc tcgaacagga tgccctcgtc gtggaggtcc tcgatcgcgt agccctgcgg
      301 gatgccgcgc cacggcagga tctcctcgtc gaactcggcg aggatgccgg ccgccgggtg
      361 gatcgagagg ttcttgccga gctggcccga ggcggtgccg agctgctggg cgccgagcag
      421 gagcggggtc atgatcgcgc cgcccgcgac gacgacggcg cgggcgtgga cggtgagcac
      481 gtggccggcg tcgctggtgg cgaccacgcc gcgcgcccgg ccgccctcga cgatgatccg
      541 ggtgacgcgc aggccgggga acagctcggc gccggcgcgc agcgcgagcg ggatgtagct
      601 gacgttggtc gagcgcttgg cgtcggtcgg gcagccgaag cagcacacgc cctggccgtc
      661 gcacgccggc gcgttgcggc gcagcgggcc gtgccgggtc aggccgaggg actcgcagcc
      721 gcgcgcgatg acgcggccgt tgccgccgag cagctcggcg cgggcgcgct cgacgtggag
      781 cacggcctcg acgcgctcga agtggcgggc catcgcgtcg ggcccgagct cggtgaggcc
      841 gagctcgtcg cgccagcgct cgagcacgcg gtcgggggcg cggtagcagg tccccgagtt
      901 gacggtggtg gtgccgccga cggtctggcc gagcgggatc gggatgccga cgttgccgat
      961 cgagaacgtc gagccgccgc ggcggtacag cttctgctgc atctcgaagc tgcggccggt
     1021 gaagtcgctg cggtcgaagt agcggccctg ctcgacgaac accaccgcga ggccggcctc
     1081 ggcgagctcg cggccgacca ccgcgccgcc ggcgccggtg ccgaccacga cgacgtcgca
     1141 ctcgaccgcg agatcgccgt cgagctggtg ggtgcgatcg cggaggtacg ccggcttggt
     1201 ctcgcggccg cgcgtggcct cgtagacgca gccgagccgc tggtacagcg ccggatcgtc
     1261 gaagtgcgcc atcttgagcg ggctgaccag cacgcgcagc agcagccggc ggatcggatc
     1321 ggcggtgcgc cagccgtcga gcagcttgag ccgccgggcg atcggcagcc gggcgaacgg
     1381 ccggcgctcg gtgagccagg ccgcggcgtc gagcgagcgc aacagcccgc cgaccccgcg
     1441 gcgcagcgag tccggcagct cgccgaggaa ctgctcgacc ttctgcaccg tcgtctcgcc
     1501 ggccgccggg aggtagcggc ccgcggggag ggccgcctcg gcgacggcga ccaggttgcg
     1561 gtgatggact gcggcgagag gaggcaccaa ggccagctta gcgcaaaatg gtcggaccag
     1621 tggaccaaag tggatggcag ccactgcctg agggcgtaac ccctggacct tgtgggattt
     1681 cagggcgtgc gccgacgcgc gaggcgggct tggtcagaac aggacctcgt tccactccgc
     1741 gaccgtccag cgcgcgacga cctggtggac gatgaacgga tcgccggcga tgaagtcctc
     1801 ggcggcctgc cggctggtga acacgccgaa cgcgcggccg ctgccgtcca tcaccgggcc
     1861 ggccatcagc agcgtgccgc gcgcgtggaa ctggtcgagc cgatcgcggt gcgcggggag
     1921 gtgctcgcgc gcccgggcca gcccctcggg ggtggtctcg tagatcatga ggaacttgcc
     1981 gcgcgcgctc gcgccggcga acgcgtggag cgacgcggcc cacgcgtcgt cggcctcgaa
     2041 cgtcttcttc atggaatcgg cgtcggcgcc gtagcgctcg agctggcggt gctcgagctc
     2101 gacccgcgtg cgccgcggac cctcggcgag gaacccgacc tcgatctcgg tggagaacgc
     2161 cgggtcgtac tgccaccggg cggtgatctg ccaggtcagc accagccggt ggggtggctc
     2221 ccaggcgacg accttgccga cgtcgacctc gctgccgtcc ttgcagatcg agtaccagcg
     2281 gccgccggcg cgcggctcga cgaccatgcg ctcgagcggc gatgcgccga tgtgatgctc
     2341 gcgcgaccac cagcgatcga tgccgtcggt gaaggtgcgg aaggcgcgct gctgggaagt
     2401 ctcgaccacg atctgcttct tgacgctggt cacgtgtcct cctgttcggc tgcgagcttg
     2461 aaggcggcga gcgcgcggtc ccagaaccga tcgaggtaag cgcgcatcgc cgccacgccg
     2521 cgcggatcga cctggtagat gcagcggttg ccgtcgcggc gctggaccac caggccggcg
     2581 cgcagcagga ccttgaggtg ctgcgacacc gccggccgcg tgacgtcgag gccctcggcg
     2641 agcttgccga ccgagctcgg ccgccgggcg acgcgctcga agatcgtccg gcgcgtgtcg
     2701 tcggcgagcg cggcgaatcc gagctggtta gccattgctt accgtaagtg tccgcttacg
     2761 aagacgtcgc tgtcaagccg ctcgcgcgcc ggcgcgcatg gcgtgcatcg ggcgatgtcc
     2821 ctcaggagac cacgcaatcg acgcgctcgt ggcgatgctt agcgtgggga catgcaaacg
     2881 catacctggt tgatctttgc agggctcgcg ctgggggcgg gctgcacggg cctggtgaac
     2941 gagagctcgg ccgagctggc ggatccgtcg acggtctcgt cggcgagcgc cgacggctcg
     3001 gccgcggcgg cgcagttctc ccccagcgcc gcgatcgcgg gcgtctggga tccgcggttc
     3061 acgatcgccg gcttcaccgg ccccgatggc cacgcgccga cggtctacga cttcgcgcgg
     3121 gacatcgacg gctcgatcgt cgcgaccggc gagttccgca ccttcggccg cacgcgggtc
     3181 gagccgctgc tccggctgcg caacggggtg tggcagccgg cgcgcgcgcg ctgggagatc
     3241 ccgccgccgg cctcggggtt ctcggcggtg gcgatcgcgc ccgacggcaa gctcgcgctc
     3301 gcgacctacg acgacttcgg tccgcgcgcc ggacagatct ggctcgacga cggcaccggg
     3361 ctgcgggtga tcgggacgtt cgacggcctg atccgccggc tgcgctggta ccgcggccag
     3421 ctgtgggcgg ccggctggga tcagatgcac cagggcgcgt cgcggatcca gggcctcgcg
     3481 gtgtggaacg gcgcggcgtg gaccgcgccg gtgggcggcg cgccgaccgg gttcgcgttc
     3541 gagctggtcg aggacgccgg cgagctcctg gtcggcggcg acttcacgtc ggtcggcggt
     3601 gtgccgtcca gcggggtctc ggcgtggacc ggcaccgcgt ggcacccgat ggggttctcg
     3661 cgcggcgcca cggtctacac gctgacccgc gggccggatg gccagctcta cgccggcggg
     3721 gcgctcggcg acctcggcgg cggcgcgggc ggcatcgcgc gctggaccgg cgagggctgg
     3781 gtcctggtcg gcggcggcgt ggtcaaccgc acgacgccgg gggtggtgac cgcgctgacc
     3841 tcgcaccagg gctcgctgtt cgcggcgggc tgcttcgtca ccgccggcgg cggggagggc
     3901 atgccgggcg cggtggtgtc gcgcgacgtg gtgcggttcg acggtgcgtg gcactcgctc
     3961 gacgacggcg cccacggcac ggtcgcgccg tggatcgagc cgctggcgtg cggcgacgag
     4021 gggccgagct cggtgtggga cgtgtccaag caggcgatgg tcagcaccgg gcgccagctc
     4081 ctgctcggcg gatcgttcgc cgggatcgcg ggcgtcgagt cgcaggcgat cctcgcgaac
     4141 aacggcgcgg gctggctcgc ccagggcgac acccggggca tcggcatcgg cggctcgctc
     4201 gaccgcctcg gcgtcgccgc gctgaccggc cagctgtggg ccgccgggca gttcagccag
     4261 gtggccggga tggcgacccg cgggcacgtc gtgcggttca ccggcgacgg ctgggtgccg
     4321 atcggcgacg cgatcccgcg cgacgcgagc tgcctggcgt tcgcggtcgc gcccgccggc
     4381 gaggtcgcgc tgggctgcac gacgttcccc agccagggca acgcggtcgg ccaggtgttc
     4441 cgcgtccagg gcgaccggct ggtccaggtc ggcggcgacc tgccgccgat ccaggccgtg
     4501 acctacgatc cgtggggccg gctgtgggtc gccggcggcg gtggcaccgg cttcgtggcg
     4561 cggctcgacg gggaggcgtt cacgctgatc gagagccggt tcgacgcgcc ggtgtcgcag
     4621 atcgacatcg ccggctcgaa cgacgtgatc gccgccggcg cgttctcgat ggtggcgggc
     4681 ctgcccgtgg cgcacatcgc gcgctggaac ggcgcgacct ggcggccgct cgggtccggc
     4741 gtgcccggcg ccgtgaccgc gctcgcgcac ggcggcggga tcgtgtacgt cagctcggtc
     4801 aacgacggca gcggcgcgct gctgctcggc gcgttcgacg gcatcacctg gcacgagctg
     4861 gcgaccccgg gggccggcct gaccccgcag ccggtgttca acttcaacgc gctgcagccg
     4921 atcgccggcg gcgtgatcgc cgtgggcacc gcccagctcg acaacggctc gggccgcggc
     4981 gcgctggtct accgcggcgg ccggttcacg gcgctcggcg gcggggtcca cgcgatcggg
     5041 ctgtccgggc tcgcggtc
//
LOCUS       DASVOR010000969         3350 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_10312,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000969 DASVOR010000000
VERSION     DASVOR010000969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10312"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VF516_18750"
     CDS             complement(<1..931)
                     /locus_tag="VF516_18750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109781.1"
                     /translation="MAPSTTQPGLRALNYHTPIGHTSFGPIFLAAGGSGGAGISFIVD
                     DRDDDSDPFDDGGDGDEDDDDGDDEIDDGDEPDPRDRRREASRTSRGQRGDVEDGEAW
                     KPPTQQQWTRVQDALKKANAEAGRRRVIGRHMDKLGIGDDLSGWLLDRGIDPETGRPL
                     GDDGGGDESFYDDGSGGEQGQRSRAELVADYRRSEQRGAARTEALYKPGIALYAAEAA
                     LREAGWVGKRMDLALQLIDPGQLDIAFDEETRWPAVTGLTEQIEAIMDEFPDWFADTR
                     RPATASRRRRDEYDEYEERSAPRRRPPARTGGAR"
     gene            complement(1065..1499)
                     /locus_tag="VF516_18755"
     CDS             complement(1065..1499)
                     /locus_tag="VF516_18755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109782.1"
                     /translation="MFPGANASVYHNEAGEPLGWDTPDDDGPYDPDAYLMGDYDDQEP
                     DDDDDEGDDEHIQPPTQDGMRAWLGDVYAQLTPGQFDDVMIAARDLAGQYGPDQRTEY
                     DGALSEFVQALIGDDGHDYEPGEHTHPMHTDPDCRICGGRKH"
     gene            complement(1558..2301)
                     /locus_tag="VF516_18760"
     CDS             complement(1558..2301)
                     /locus_tag="VF516_18760"
                     /inference="COORDINATES: protein motif:HMM:NF022432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HEX8109783.1"
                     /translation="MTTPSGDTRWLETVRKLLAKAEDPACPPAEAEALTAKAAEIVAK
                     YGIDQALLNANRAENRAKPEIKRIMIYGPSYTYEKATMLGRIAEVLGCRAVLHAYGRN
                     ADSVSLVGFAADIERAEILYTSLLIQQANALTQEYIPYSGNRAAAWKRSWMDAYSGAV
                     AMRLRSAERRAQQGAEAERGPGTDLVFVGRADEVRQMYEDEFTDLTKRRRQLTGDGRR
                     AGYAAGARANLGGTGVTSSTPNRSLSGRR"
     gene            complement(2353..2907)
                     /locus_tag="VF516_18765"
     CDS             complement(2353..2907)
                     /locus_tag="VF516_18765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109784.1"
                     /translation="MTRTIIRKSQAPAASWTIAWRKRTANHFRRANNWAGTWAEAFEM
                     ARAFGELHPEYQVYYTTTAAYEQWERDEIAAGTLAADRGEDHGNIMVDSGKRIRVRDT
                     GVVEADLLDADIAKVAAEVHETSYVRKNGITSQNPRSRGQWYTSPAKCREWARGVRAS
                     YPEIFASMVTDSRTADERFAAGAN"
     gene            complement(2983..>3350)
                     /locus_tag="VF516_18770"
     CDS             complement(2983..>3350)
                     /locus_tag="VF516_18770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109785.1"
                     /translation="YPTCLGYAGALAAPGEPFRPVFSVGDPSARPAGPVHGPPLHPWC
                     RCELDWWYPDGPDLTPLDLPYALRREAERSILTGQAQGSAPARLRAADRLVDLAGLIV
                     PKTVVKRAKKALQAGRFPR"
BASE COUNT          366 a         1142 c         1270 g          572 t
ORIGIN      
        1 ggcgggcacc gccggtgcgg gccggcggcc ggcgccgggg ggcggaccgc tcctcgtact
       61 cgtcgtactc gtcgcgacgg cgccgcgagg cggtggctgg ccggcgggtg tcggcgaacc
      121 agtcggggaa ctcgtccatg attgcctcga tctgttcggt gaggccggtc acggccggcc
      181 agcgggtctc ctcgtcgaac gcgatgtcga gctgccccgg gtcgatgagc tggagtgcga
      241 gatccatgcg cttgccgacc cagccggcct cccgcaaggc ggcctcggcc gcgtacaggg
      301 cgatgcccgg cttgtacagg gcctcggtgc gggccgcgcc gcgctgctcg ctgcgccggt
      361 agtcggcgac cagctcggca cggctgcgct ggccctgctc gccgccgctg ccgtcgtcgt
      421 agaaactctc gtcgccgccg ccgtcgtcgc cgagcggccg cccggtctcg gggtcgatgc
      481 cccggtcgag cagccagccg gacaggtcgt cgccgatgcc gagcttgtcc atgtgccggc
      541 cgatgacgcg gcgccggccg gcttcggcgt tggccttctt cagggcgtcc tgtacgcggg
      601 tccactgctg ctgggtgggc ggcttccagg cctcgccgtc ctcgacgtcg ccgcgctggc
      661 cccgactggt gcgggatgcc tcgcggcggc ggtcgcgggg gtcgggctcg tcgccgtcgt
      721 cgatctcgtc gtcgccgtcg tcgtcgtcct cgtccccgtc gcccccgtcg tcgaaggggt
      781 cggagtcgtc gtcgcggtca tcgacgatga agctgatccc cgcgccgccg gagccgccag
      841 cggccaggaa gatcggcccg aacgaggtgt ggccgatggg ggtgtggtag ttgagggcgc
      901 gcaggcccgg ctgtgtggtg ctgggtgcca tctcggcagc ctcctggggt gcttctgccc
      961 gtcgcgggct gacgtctcgt cttgaacgcc cagggtagcc gggtttgttc agtaacggaa
     1021 cgaacccccg ggcgggccga gggttcgtgc gcttcggggg ctggtcagtg cttccggcca
     1081 ccgcagatcc ggcagtccgg atcggtgtgc atcgggtggg tgtgctcgcc cggctcgtag
     1141 tcgtggccgt cgtcgccgat cagggcctgc acgaactcgc tgagcgcccc gtcgtactcg
     1201 gtccgctggt cggggccgta ctggccggcc aggtcccgcg ccgcgatcat gacgtcgtca
     1261 aactggccgg gggtgagctg ggcgtagacg tcgccgagcc atgcccgcat gccgtcctgg
     1321 gtgggcggct ggatgtgctc gtcgtcgccc tcgtcgtcgt cgtcgtcggg ctcctggtcg
     1381 tcgtagtcgc ccatgaggta ggcgtccggg tcgtacgggc cgtcgtcgtc cggggtgtcc
     1441 cagccgagcg gctccccggc ctcgttgtgg tacacgctcg cgttcgctcc agggaacatg
     1501 atcggatcct ctcgataggc ctggagcccc gggacgtccc ggggctccag ggatgcttca
     1561 gcggcggccg gagaggctgc ggttgggggt gctggaggtg acgccggtac cgcccaggtt
     1621 ggcgcgggcg ccggccgcgt agccggcgcg gcggccgtcg ccggtgagct gccggcgccg
     1681 cttcgtgagg tcggtgaact cgtcctcgta catctgacgc acctcgtccg cgcggccgac
     1741 gaacaccaga tcggtacccg ggccgcgctc ggcctcggcg ccctgctggg cgcgccgctc
     1801 ggcgctgcgc agccgcatgg cgacggcgcc gctgtacgcg tccatccacg agcgcttcca
     1861 ggcggcggcc cggttgcccg aatacgggat gtactcctgg gtgagggcgt tggcctgctg
     1921 gatgagcagc gaggtgtaca ggatctcggc ccgctcgatg tcggcggcga agccgaccag
     1981 gctcacgctg tccgcgttgc gcccgtaggc gtgcaggact gcccgacatc cgagcacctc
     2041 cgcgatgcgc ccgagcatgg tggccttctc gtaggtgtag ctaggcccgt aaatcatgat
     2101 gcgcttgatc tccggcttgg cccggttctc ggcccggttg gcgttgagca gtgcctggtc
     2161 gatgccgtac ttggcgacga tctcggccgc cttggcggtg agcgcctcgg cctcggccgg
     2221 cggacacgcc gggtcctcgg ccttggccag gagcttgcgg acggtctcca gccatcgggt
     2281 gtcaccgctg ggggtcgtca tctcgggtgg tcctctcggt cggttgggtg ggggaagggg
     2341 tggcgccggg gctcagttgg ccccggcggc gaagcgctcg tcggcggtgc gggagtcggt
     2401 caccatcgag gcgaagatct ccgggtagct ggcgcgcacg ccgcgcgccc actcgcggca
     2461 cttggccgga ctggtgtacc actggcctcg ggaacgcggg ttctgcgaag tgatgccatt
     2521 cttgcggacg taactggtct cgtggacctc ggcggccacc ttggcgatgt cggcgtcgag
     2581 caggtcggcc tcgaccacgc cggtgtcgcg gacgcggatc cgcttccccg agtcgaccat
     2641 gatgttgccg tggtcctcgc cccggtcggc ggcgagcgtg ccggcggcga tctcgtcgcg
     2701 ctcccactgc tcgtaagcgg cggtcgtggt gtagtacacc tggtattcgg ggtgcagctc
     2761 gccgaacgcg cgggccatct cgaacgcctc ggcccaggta ccggcccagt tgttggcgcg
     2821 tcggaagtgg ttagcggtgc gcttgcgcca tgcgatggtc cagctcgcgg cgggggcctg
     2881 gctcttgcgg atgatcgtgc gggtcatctc ggtctccctg ggctcgtcgt tcgcttgcga
     2941 acacgagctt acacaggaac acgcggcgtg tcaactgccc ctctacctgg ggaaacgccc
     3001 cgcctggagg gccttcttgg cgcgcttgac gaccgtcttg ggcacgatca ggccggccag
     3061 gtcgacgagg cggtcagcgg cgcgcagccg cgccggggcc gagccctgcg cctgcccggt
     3121 gaggatcgag cgctccgcct cccggcgcag cgcgtacggc aggtccagcg gggtgaggtc
     3181 cggcccgtcc ggataccacc agtccagctc gcagcggcac cacggatgca gcggcggccc
     3241 gtgcaccggg ccggccggcc gcgccgacgg gtcgccgacg ctgaacacgg gacggaacgg
     3301 ctccccgggg gcggccaggg cgcccgcgta cccgagacac gtcgggtagg
//
LOCUS       DASVOR010000970         2066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_389432,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000970 DASVOR010000000
VERSION     DASVOR010000970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389432"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..465)
                     /locus_tag="VF516_18775"
     CDS             complement(52..465)
                     /locus_tag="VF516_18775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109786.1"
                     /translation="MRRGRILAIKWGMNPNSSSLGIDVTFLLVGATVLAAVTPVIGAL
                     LHWRRPVAAEAAAAAGVAAVGQLQVGLERRRQRRVADRDLGARPDRRLARSDRHRAAR
                     ERPVDQHQRARHRQLDDRAEPLDADPFRRVQLRRA"
     gene            complement(616..888)
                     /locus_tag="VF516_18780"
     CDS             complement(616..888)
                     /locus_tag="VF516_18780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109787.1"
                     /translation="MTTYEILVDPQRLLRVRFVDATRTEQNLVALTCAYVPVRGTTGQ
                     LQVAVEVVVDPAMQNPGVAYYCDPQRLQHHVIPPGHLRGIHPYLSQ"
     gene            complement(885..1481)
                     /locus_tag="VF516_18785"
     CDS             complement(885..1481)
                     /locus_tag="VF516_18785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109788.1"
                     /translation="MITFESQLANEHRARGFFRELPDPQVASAIIAEQYWDDDDQARQ
                     PTLRRILQRPNVSAIAYPTLGHRDVLQVFLRDSGAEPVFACVLVSGARLRDMLTGSSP
                     IVEGFALQVLPSEHLSVESVRAALQSWIERCFPDLVLPSLVVERWTGPEGILQELLEL
                     LLAPPGVDPATLIVIYADGADELHPDPALAQPPYELAA"
     gene            complement(1499..1867)
                     /locus_tag="VF516_18790"
     CDS             complement(1499..1867)
                     /locus_tag="VF516_18790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109789.1"
                     /translation="MVVRLCRVDADPACGWSPHHFKHFPFAGMTKEHTLPPNPARYLP
                     GFDAERIREIETGTARAPGIRRERTSSKSEYQRDMLQVIGWDLGQDATLSFVECAGGD
                     VARTFHGRPMCIGNLKAANF"
BASE COUNT          323 a          667 c          741 g          335 t
ORIGIN      
        1 gttgcgatcg cgcggcgcgt tctcgacggc gtcgcgagtg cgcggcaacg gctacgcgcg
       61 gcgaagctgc actcgccgaa acggatccgc gtcgagcggt tcggcgcgat cgtccagctg
      121 acggtgccgc gcgcgctggt gttggtcgac cgggcgctcg cgcgccgcac ggtggcgatc
      181 ggagcgggcg agccggcgat ctgggcgggc cccgagatcg cggtcggcga cgcgccgctg
      241 tcggcgccgc tcgaggccca cctgcagctg accaaccgct gcgacgccgg ctgccgcggc
      301 tgcctccgcc gccaccggcc gccgccagtg cagcagcgcc ccgatcaccg gcgtcaccgc
      361 ggcgagcacc gtcgcgccga ccagcaggaa cgtcacgtcg atccccaggc tcgacgagtt
      421 ggggttcatc ccccacttga tcgccaggat ccgtccgcgg cgcaccccgc gatggtagca
      481 gagatggccc cggagtcgtc agcgccgccg cgccgcggct cacgctcgtg cgatcgcttc
      541 cgcagcgtcg atcgtctcga cgcgtccgtc gcggatctca gcgcttgctc gatcaccgct
      601 tcgatggtca cgaagtcact gcgacaggta ggggtggatg ccgcgtagat gccctggagg
      661 gatgacgtga tgctggagtc gctgcgggtc gcagtagtat gcgacgcccg ggttctgcat
      721 cgcggggtcg acgacgacct ccacggctac ctggagctgg ccggtggtgc cgcggaccgg
      781 gacgtacgcg catgtcaagg cgaccaggtt ttgctcggtg cgcgtcgcgt cgacaaatcg
      841 cacgcggagc agccgctgcg gatcgacgag gatctcgtag gtggtcatgc ggccagctcg
      901 tagggcggct gtgcgagtgc cggatcgggg tgcaattcat cggctccgtc tgcgtagatg
      961 acgatcagcg tggccgggtc gacgcccggg ggcgcaagca gcagctcgag aagctcctgc
     1021 aagatccctt cgggaccggt ccagcgctct acgacgagac tcggcagcac gagatcgggg
     1081 aagcagcgtt cgatccagga ttgcagggcg gcgcggacgc tctcgacgct gaggtgctcg
     1141 ctcggcagga cctgcagtgc gaacccttcg acgatcggcg atgaaccggt cagcatgtct
     1201 cgcaatcgcg cgccgctgac gaggacacac gcgaacaccg gctcggcacc gctgtctcgc
     1261 aagaagacct ggagtacatc gcggtgaccc agcgtggggt aggcgatggc ggagacgttg
     1321 gggcgctgga ggatcctccg caacgtgggt tggcgtgctt gatcgtcgtc gtcccagtac
     1381 tgctcggcga tgatcgcgct ggcgacctgc ggatctggca actctcgaaa gaagccacgc
     1441 gcgcgatgtt cgttagcgag ctgcgattca aacgtgatca tggctgggtc cgcgcgatct
     1501 agaagttggc tgccttgaga ttcccgatgc acatcggccg gccgtggaat gttctcgcga
     1561 cgtcgccgcc ggcgcactcc acgaacgaca aggtcgcatc ttgccctaga tcccaaccga
     1621 tgacctgcag catgtcgcgt tggtactccg acttcgagga cgttcgctct cgtcggatcc
     1681 caggcgcgcg tgcggtgccg gtctcgatct cacggatgcg ctccgcgtcg aagccgggaa
     1741 gatatcgagc tgggttcggc ggaagcgtgt gctccttggt catccccgcg aacgggaaat
     1801 gcttgaaatg atgcggactc caaccacagg ctgggtcagc atcgactcgg cacagccgca
     1861 cgaccatggt ttgtcctagc atgggatctg cgcgtcaggt ctccgggccg aagcggacgg
     1921 tctcgatcag ggcgtcgagc aggttgtcgc ggatgagcgc gatgtacgcc agggcggcga
     1981 cgccggcgag gcaggcgagc acgtgcccgg cggcgcgcag ccggcgcggg cggccggtgg
     2041 tgcgcagctg ctcgagggtg gcgccg
//
LOCUS       DASVOR010000971         2890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_477508,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000971 DASVOR010000000
VERSION     DASVOR010000971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477508"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..1129
                     /locus_tag="VF516_18795"
     CDS             59..1129
                     /locus_tag="VF516_18795"
                     /inference="COORDINATES: protein motif:HMM:NF015500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HEX8109790.1"
                     /translation="MGRRDRPAPRPAQARGCVLGFAVGAALAGCGSNPGNSPDAGQGP
                     VPPAVWQPKPGDAPNWDIQLAAPFDVSAPRTMYDLDLWSLVPSPTTLDYGDGKPVTVP
                     RGALAGTIAQLHARTPPAIVICYVETGMLDLQRPDAVKFLGYDPDPTQIPDNKDVMNT
                     AVKGAPASGSVIGWSLGDVKLRFLDARQASRAQWAPILFKRFDLALQIGCDGVDPAHN
                     DSAAYATGFNITSDDALTWYAEVAQEGHARKLSTGMRNSVTLLESIDPGANQFDWLIL
                     ERCGELEAGGTQGCDTARPFLNAQKDVFAIDYNVAQDGTAQTATTVCAPQLKETIVDG
                     LYKDAPPTGKTMIRQQCGTLPP"
     gene            complement(1136..1783)
                     /locus_tag="VF516_18800"
     CDS             complement(1136..1783)
                     /locus_tag="VF516_18800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109791.1"
                     /translation="MSARSSARDLGLLACAAAAACSTTHKPEADPARVASLMKAIDQH
                     TPGPGDVPDCKPSEMIGGATLTQVTVLKIGGVPAHPGAEREDWMNPPELDSPAARELL
                     DPAVDDTTRRQAAYELLGAPFYLVYRIELVDAPMALGLKDIRRGVVSMRALRFDRDGN
                     SVCGKVVTYQQSLAKSDWAIVKSNLAVIDPKVSKVLRDDLHDLLLKHVAALGRPD"
     gene            complement(1758..2561)
                     /locus_tag="VF516_18805"
     CDS             complement(1758..2561)
                     /locus_tag="VF516_18805"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109792.1"
                     /translation="MTAERRTLDSALAGSPMTGPGDRVDYRPTAVMPDVKVLKIGGQS
                     VLDRGRAALFPILDEIVEARKLGIQIVVLAGGGTRARHIYSIASELEVPTGVVATLGK
                     YIPMQNARMLQMLLAKHGGIFMLPDDFEKLPLYLQMGCIPVMSGMPPFGYWEKREEGS
                     RIPPHRTDAGVFLSAEFLGARRALFIKDEDGLYTDDPKKNPAATHIPSITAAELMARG
                     LPDVVLERVVIEYLPRAQWCKELQIVNGLKPGQVLAALRGEDVGSIIRA"
     gene            complement(2558..>2890)
                     /locus_tag="VF516_18810"
     CDS             complement(2558..>2890)
                     /locus_tag="VF516_18810"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109793.1"
                     /translation="HLPMHGSDFGLFQLAEVLGMKQVIYVKDEDGLYDRDPKKHPGAR
                     KFERLTLDELLPQLPDENILDRELFHAWSRAKNLERITIVNGLVRGQLTRAMRGEDVG
                     TVIVKAAP"
BASE COUNT          430 a          957 c         1064 g          439 t
ORIGIN      
        1 gatcacaagc ggtgcaactc ggcgccacgg cgcggaccga ggggcgtagg atccgtccat
       61 gggacgacgt gatcgaccag ctccgcgccc ggcgcaggcg cggggctgcg tgctcggatt
      121 cgcggtgggc gcggcgctcg cgggctgcgg tagcaatccg ggcaactctc cggatgccgg
      181 ccagggtcca gtgccgccgg cggtgtggca gccgaagccg ggcgacgcgc cgaactggga
      241 tatccagctg gccgcgccgt tcgacgtctc ggcgccgcgc acgatgtacg acctcgatct
      301 gtggtcgctg gtaccgtcgc cgacgacgct cgattacggc gacggcaagc cggtcacggt
      361 gccccggggc gcgctggccg gcacgatcgc gcagctgcac gcgcgcacgc cgccggcgat
      421 cgtgatctgc tacgtcgaga ccgggatgct cgacctccag cgtcccgatg cggtcaagtt
      481 cttggggtac gacccggatc cgacgcagat cccggacaac aaggacgtca tgaacacggc
      541 ggtcaagggc gcgcccgcgt cgggcagcgt gatcgggtgg agcctgggcg acgtcaagct
      601 gcggttcctc gacgcccgcc aggccagccg cgcgcagtgg gccccgatcc tgttcaagcg
      661 cttcgacctg gcgctgcaga tcggctgcga cggcgtcgat ccggcgcaca acgactccgc
      721 ggcgtacgcc accgggttca acatcacgtc ggacgacgca ctcacctggt acgcggaggt
      781 cgcgcaggag ggccatgccc ggaagctctc caccggcatg cggaacagcg tcaccctgct
      841 ggagtcgatc gacccgggtg ccaatcagtt cgactggctg atcctcgagc gctgcggcga
      901 gctcgaggcg ggggggacac aaggctgcga cacggcgcgg ccgttcctca acgcgcaaaa
      961 ggacgtgttt gcgatcgact acaacgtcgc gcaggacggc acagcgcaaa cggcgacgac
     1021 ggtctgcgcg ccccagctga aagagacgat cgtggacggc ctctacaagg acgcgccgcc
     1081 caccggcaag acgatgatcc gccagcagtg cggaacgctg ccgccgtgac cgccgtcagt
     1141 cgggtcggcc gagcgccgcg acgtgcttga gcagcaggtc gtgcagatcg tcgcgcagca
     1201 ccttggagac cttgggatcg atcacggcga ggttgctctt gacgatcgcc cagtcgctct
     1261 tggccagcga ctgctggtac gtgaccacct tgccgcagac gctgttgccg tcgcgatcga
     1321 agcgcagcgc gcgcatgctg accacgccgc ggcggatgtc cttgaggccg agcgccatgg
     1381 gcgcgtcgac cagctcgatc cggtagacca ggtagaacgg ggcgccgagc agctcgtagg
     1441 cggcctgccg cctggtggtg tcgtcgaccg cggggtcgag cagctcgcgc gccgcggggc
     1501 tgtcgagctc ggggggattc atccagtcct cgcgctcggc gccggggtgc gccgggacgc
     1561 cgccgatctt gagcaccgtc acctgggtga gggtggcgcc gccgatcatc tcgctcggct
     1621 tgcagtcggg gacgtctccc gggccggggg tgtgctggtc gatcgccttc atcagcgatg
     1681 cgacccgggc cggatcggcc tcgggcttgt gggtcgtcga gcacgcggcc gccgcggcgc
     1741 aggcgagcag gccgaggtca cgcgcggatg atcgagccga catcttctcc ccggagcgcg
     1801 gccagcacct ggccgggttt gagcccgttg acgatctgga gctccttgca ccactgcgcg
     1861 cgcggcaggt actcgatcac gacgcgctcg agcacgacgt cgggcaggcc gcgggccatc
     1921 agctcggcgg cggtgatcga cgggatgtgg gtcgccgccg ggttcttttt ggggtcatcg
     1981 gtgtacaggc cgtcctcgtc cttgatgaac agcgcgcgcc gggcgccgag gaactccgcc
     2041 gacaggaaca cgccggcgtc ggtgcggtgc ggcgggatcc ggctgccctc ctcgcgcttc
     2101 tcccagtagc cgaacggcgg catgccgctc atgaccggga tgcaccccat ctggaggtac
     2161 agcggcagct tctcgaagtc gtcgggcagc atgaagatgc cgccgtgctt ggcgagcagc
     2221 atctgcagca tgcgggcgtt ctgcatcggg atgtacttgc cgagcgtggc gaccacgccg
     2281 gtcgggacct cgagctcgga ggcgatcgag tagatgtggc gcgcgcgggt gccgccgccg
     2341 gcgagcacga cgatctggat gccgagcttg cgggcctcga cgatctcgtc gaggatcggg
     2401 aacaacgcgg cgcggccgcg atcgagcacg ctctggccgc cgatcttgag caccttgacg
     2461 tcgggcatga ccgcggtggg gcggtagtcg acgcggtcgc cggggccggt catcggtgag
     2521 ccggcgagcg cggagtccag ggtgcggcgc tcggcggtca cggcgccgcc ttcacgatca
     2581 cggtgccgac gtcctcgccg cgcatcgcgc gggtcagctg gccgcggacg aggccgttga
     2641 cgatcgtgat ccgctcgagg ttcttggcgc gggaccaggc gtggaacagc tcgcgatcga
     2701 ggatgttctc gtcgggcagc tgcggcagca gctcgtcgag ggtgagcctc tcgaacttgc
     2761 gggcgccggg gtgcttcttg ggatcgcggt cgtacaggcc gtcctcgtcc ttgacgtaga
     2821 tgacctgctt catgcccagg acctcggcga gctggaacag gccgaagtcc gagccgtgca
     2881 tcggcaggtg
//
LOCUS       DASVOR010000972         2294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447952,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000972 DASVOR010000000
VERSION     DASVOR010000972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447952"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VF516_18815"
     CDS             complement(<1..735)
                     /locus_tag="VF516_18815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8109794.1"
                     /translation="MAKKSSSKGGEGAPPGSGGGGGGGAAPGDIDASLETEARRRYLN
                     YALSVITSRALPDVRDGLKPVQRRILYAMLHDNHLRPDAKYRKSATVVGAVLGRYHPH
                     GDTSVYDAMVRMAQDWAMRATMIDGSGNWGSLDGDEAAAMRYTECRLAPAAMELLREL
                     DFETVDMRPNYDGTTEEPVVLPARFPNLLVNGSTGIAVGMATNIPPHNLSEVCTALVK
                     LLDNPDLSSVQLCRSVKGPDFPTGGEM"
     gene            876..1469
                     /locus_tag="VF516_18820"
     CDS             876..1469
                     /locus_tag="VF516_18820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109795.1"
                     /translation="MVTKRRTGRKKRSVRPIGNLGGSPARRTGGWVVLVLAALVVVNL
                     YVFVWDKQTSVGAIRREADRATPTATVPPHPLAEPGAGSGSAPPSAPAPPSDPPGVIQ
                     GKVGKSDTLGRVLKKSGLSAAEADEVIRALSGVFDFKTMRAGQAFRIERGPDGRVKSF
                     ELAVGKTGNVRADRGADGALVGKAAGAQPRSETNPRP"
     gene            1466..2020
                     /locus_tag="VF516_18825"
     CDS             1466..2020
                     /locus_tag="VF516_18825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109796.1"
                     /translation="MILAPGENVLADASLPRQTCKVFKLVVGILKGAIIGGALGYGAF
                     ALKEATELANPWLTYGAVGALVGLLAGRPIWSLLRDKNATAWVSILKAAFGFGVGCGL
                     YAIIAKLWTAPEVVVQGYDIFTWPVTLGGAIGAVYGGFVELDDAIGDDKKLAGDPARP
                     GTGAGTKTSAAAAAAKAKAAPPKR"
     gene            complement(2047..>2294)
                     /locus_tag="VF516_18830"
     CDS             complement(2047..>2294)
                     /locus_tag="VF516_18830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109797.1"
                     /translation="LSPSLGDLGGHTLGLGLEATTGGVTTTIGWSRTWSPETSPLSEL
                     RLDSPFPYSSDGPVLPGKYAGAVDQVGVLIEAEIGGR"
BASE COUNT          327 a          791 c          830 g          346 t
ORIGIN      
        1 catctcgccg ccggtgggaa agtcggggcc cttgacggag cgacagagct ggacgctgct
       61 cagatcgggg ttgtcgagca gcttgaccag cgccgtgcag acctcgctca ggttgtgcgg
      121 cgggatgttg gtcgccatgc cgacggcgat cccggtcgat ccgttgacca gcaggttggg
      181 gaaccgcgcc ggcagcacga ccggctcctc ggtcgtgccg tcgtagttgg gccgcatatc
      241 cacggtctcg aagtcgagct cgcgcagcag ctccatcgcg gcgggagcga gccggcactc
      301 ggtgtagcgc atcgccgccg cctcgtcgcc gtcgagcgat ccccagttgc ccgagccgtc
      361 gatcatggtc gcccgcatcg cccagtcctg ggccatgcgc accatggcgt cgtacaccga
      421 cgtgtcgccg tggggatggt agcgacccag cacggcaccg accacggtcg cgctcttgcg
      481 atacttggcg tcgggacgca agtggttgtc atgcagcatc gcgtacagga tccggcgctg
      541 caccggcttc aggccgtccc ggacatccgg gagtgcgcgg gacgtgatga cgctgagcgc
      601 gtagttgagg tagcgccgcc gcgcctcggt ctccagcgag gcgtcgatgt cgccgggagc
      661 cgcaccaccc ccaccgccgc cgccgctacc cggtggtgcg ccctcgccac ccttcgagga
      721 agacttcttg gccatgcggg cggcaccttg ccccgactcg gggaaaagtt ctagttgttc
      781 gcgcgcgggc agatcacctg gggtccgcgg ggtaggagag gttgccattt ccttggtccc
      841 atcgaaggcc catgatattg ctggcctcct tcggcatggt cacgaagcgt aggacagggc
      901 gcaagaaaag gtcggtccgg ccgatcggca acctcggggg atccccggcc cgcaggaccg
      961 ggggctgggt cgtgctggtg ctggccgcgc tggtggtggt caacctgtat gtcttcgtgt
     1021 gggacaagca gacctcggtc ggcgcgatcc ggcgtgaggc cgatcgtgcg accccgaccg
     1081 cgacggtccc gccccacccg ctcgcggagc ccggcgcggg gtccggatcg gccccgccga
     1141 gcgccccggc gccgccgagc gacccccccg gtgtgatcca gggcaaggta ggcaagtccg
     1201 acacgctcgg ccgcgtgctc aagaagagcg gcctgtccgc cgcggaggcc gacgaggtga
     1261 tccgcgcgct gtccggcgtg ttcgacttca agacgatgcg cgccggccag gcgttccgga
     1321 tcgagcgcgg cccggacggc cgcgtcaagt ccttcgagct cgcggtcggc aagaccggga
     1381 acgtacgggc cgatcgcggc gccgatggcg cgctggtcgg caaggcggcg ggcgcgcagc
     1441 ctcggagcga gaccaacccg cggccgtgat cctcgcgccc ggcgagaacg tgcttgccga
     1501 tgcgtcgctt cctcgtcaaa cttgtaaggt gttcaagctc gtcgtcggca ttctcaaggg
     1561 tgcgatcatc ggcggcgccc tgggttacgg ggcattcgcg ctgaaggaag cgacggagct
     1621 cgccaacccg tggctcacct acggcgcggt cggcgcgctg gtcgggctgc tcgccggccg
     1681 gccgatctgg tcgctcttgc gcgacaagaa cgccaccgcc tgggtctcga tcctcaaggc
     1741 cgcgttcggg ttcggcgtgg gctgcgggct ctacgccatc atcgccaagc tgtggacagc
     1801 cccggaggtc gtcgtgcagg gctacgacat cttcacctgg ccggtcacgc tcgggggcgc
     1861 gatcggcgcg gtgtacggcg gcttcgtcga gctcgacgac gcgatcggcg acgacaagaa
     1921 gctggccggc gatccggcgc ggcccggcac cggcgccggc accaagacct cggcggcggc
     1981 ggcggcggcg aaggccaagg ccgcaccgcc gaagcgctga gccggggcct tcaccgtccc
     2041 cggcggtcag cgaccgccga tctctgcctc gatcagcacg ccgacctggt cgaccgcgcc
     2101 ggcgtacttg ccgggcagaa ccggaccgtc gctgctgtac gggaacgggc tgtcgagccg
     2161 gagctcgctg agcggagagg tctcgggcga ccaggtccgc gaccacccga tcgtcgtcgt
     2221 gacgccgccg gtcgtggctt ccagcccgag gccgagcgtg tggccgccga gatcgccgag
     2281 cgacggcgag agcc
//
LOCUS       DASVOR010000973         8540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_243644,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000973 DASVOR010000000
VERSION     DASVOR010000973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8540
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243644"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VF516_18835"
     CDS             complement(<1..279)
                     /locus_tag="VF516_18835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109798.1"
                     /translation="MLLSGGHDTRYTTDPKTASEVDGIVTLPGDNRVARTGELRLGPH
                     FAPLSRWASELTILNGVQVRTVTHDTGQKQFFHLKTNIADMMPSVLDVV"
     gene            complement(478..1344)
                     /locus_tag="VF516_18840"
     CDS             complement(478..1344)
                     /locus_tag="VF516_18840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109799.1"
                     /translation="MAAGRDARAQAWVEDKGTLELNLGYNLGISDKVIVDKGSDFADA
                     GVTTHEITLAAEYVPLEHLAVGVGLPMALLEYTGVKSAASAHPGGGRYDDGSLHATLT
                     DLRAGARYQLLEDPLALAPHIAVSIPVASYETVGNAVAGRHLKALHLGLAVGRVFGES
                     TYVHALYEFSIVEKYDRTMETAQFGQNRSDFTFTVGHKLLDQRLDLHVDVNGRVTHGG
                     MNFSEFTMLPMNDQLYHDPILAETILLVGGGVGYQLTNALGINAGARLFVTGSNTQNA
                     NVYVFGITWAAL"
     gene            1471..2466
                     /locus_tag="VF516_18845"
     CDS             1471..2466
                     /locus_tag="VF516_18845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109800.1"
                     /translation="MATTIWAHLTCLAAVAAGGCSPAPRGRIVVAITIDWEGAYLSPD
                     GLDALDDVRRSLGPAPVTHFVSAAYFTKDPPDPKAAATITQEVRKGDELAVHLHAWRS
                     LARASGIEPRLSPSFLTGTDKLLEFEDGDLGFDTDLDAYSVTDLRVLLRTSRRLLEGT
                     HLPVSRTFRAGGYLGTPKVLQAIQAEGFAVDCSATDHRQLDERKDAVLPRRLQAIWPG
                     VEPTSQPWSIDAPGDAPGGPLLEMPIAAIADYATPAQIVAVFEAAHARLRDDPRRDVF
                     VVVGFHQETAQEFAGRLREALDRIRGRREIADELMFSTVEDAAARARRTAARPPS"
     gene            2641..3609
                     /locus_tag="VF516_18850"
     CDS             2641..3609
                     /locus_tag="VF516_18850"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8109801.1"
                     /translation="MKWNFAAGLVTAIFVAMHAAPPADACGVKLTVKSSTPRKAVART
                     SNPSDILLLGNPPRRLESDLSAAGHRVDVAPTATAVKRKSYAVVITDASLLDEARSSF
                     PDAVVIVRSGDETADMRSVEQQVARKPVRTEAARSAVAARPTRTPVAAGPPQDPNRRI
                     VAASEPKDTPEPAPPPPSPAPEKVAVTTPKVETRSVEPKVTDVAPTPTPEPRARAVGK
                     PGTIHHELYFALGSFKIDPRSLARDVSWLTNNKDVQVVIEGHADPSGSHEGNMALGQK
                     RAEAVRDSLVSAGIEESRLEVISYGDTRLRYGRADGRNRRAAIVVK"
     gene            3621..4181
                     /locus_tag="VF516_18855"
     CDS             3621..4181
                     /locus_tag="VF516_18855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109802.1"
                     /translation="MSHARVVCSALGVPLLLASCGSPASHSPDAAAGPCWPLDAMPGG
                     QAELGTGDIDFEPMPAMLPIIRNASQSDPFLQIHARIHGLPPGNPDDILAPGNPRTKV
                     GATIDELGLTLGVECPASLGYVASPEAGAFDLVHSLRIGLGTIPPAQVAGKQAHITLE
                     VVGSNGLYAQDMKLVTVTLMTTSAAR"
     gene            complement(4185..5072)
                     /locus_tag="VF516_18860"
     CDS             complement(4185..5072)
                     /locus_tag="VF516_18860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109803.1"
                     /translation="MLTDPEGGQIVLEYMYLDTELQAFFKLPAGVTTLNRVSAFFMND
                     QTPEANLLPTPGQCNNLVATKGWPGYIGTPHTDLDVGTVTLTGKNTAGTDVTITVDKQ
                     PKGTDVFGRPHDIFYQAINPIAESFLKPDSPYAVKFGGSPTGATTPIPATTFDGGLYL
                     AQTFNVSNPTHEENGPLVAGTDFTVHWTPTNSPGLPAGDQLQVITWLVDVTGAPTHMC
                     PAPMTAGQFTVPGTAIAEYQAIAKARGLPFNKMVMLRNGVVHQLRRLPNNSTTNKRRI
                     DMLTLMCWAQLMDVQGTPP"
     gene            5881..7995
                     /locus_tag="VF516_18865"
     CDS             5881..7995
                     /locus_tag="VF516_18865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109804.1"
                     /translation="MTTAPIASKPLSQTRNLDKFAFEKSTWGGVTYLTLHGVLDQAFD
                     GRKLADLVRTQKVVVSLRNVRRFASWGMSEWMEFLQVNAARDLYLVECSTYAVSQINL
                     VNGLLGHAKLVSFYASYRCASCSEAHESVFIVPRDREIIRELPGNTEECSTCGGRARL
                     EEYPAAFFDTITDRPSFDIDDEVLAFFRSQLQYWLAPDLSQFRAHRRLDKGYTYLRLS
                     GDLAALPGERLLAASEGTTVVDLAGAVFEPGQLTAWRTYVRGALAKLRSLQLLDCPLG
                     FLEDAVAPEDLRDKLKIRTFGLSYDCLRCETAVTRMVGVAENLELLVDGVAPPAQCTT
                     CGSQLVAIPTARQAVVMRSLPARDRDRALDKFLAAARAEPLDKLENCLAALPAKLPTA
                     PARTSSAVFVALALALLVIGGLGAMVVALWRQRGEPARAAIAPAPAAAPKPPPTFERP
                     AWIMSDIPSSAYCYDMINRLMCIGVSSYRPTRDEAVGEANDAALDELVSAIGLKISDP
                     FFRETVMSSYAGPRAKALSALQATDLEQTSPAYAAAADAVAKARRRVAEILRASGGAA
                     VPAQRSDWYWEEYAAKKGGTEALVFIRYDVTLDAIKALVEKYSATTTVLGSTAMTAFP
                     ALAWQSADFTGGAMLTRVAHPLSDAGIAGANVVLAVGDQRVRDATSFARRIEEWKQGT
                     GELQLTVTSGEGPARVVDVKRR"
     gene            complement(8005..>8540)
                     /locus_tag="VF516_18870"
     CDS             complement(8005..>8540)
                     /locus_tag="VF516_18870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109805.1"
                     /translation="VALVNRLLAPNPDDRPQRGQDVAAELADISRQYGLSGSAPGIAY
                     VLSQLFPVETAGGAEPIQVTSTFIGAYDSKPTSSLPSSSLTLHGVPPASAALDLPPTP
                     TPRVPEPSRPVALPPPSPVPVPVSRPQVVPPVSLPSLIPPVRPSVRPSFSVVKLLVTL
                     AVAILLAVGMYMLVRPS"
BASE COUNT         1328 a         2902 c         2991 g         1319 t
ORIGIN      
        1 gacgacgtcc agcacgctcg gcatcatgtc ggcgatgttg gtcttgaggt gaaagaactg
       61 cttctggccc gtgtcgtggg tcacggtccg gacctggacg ccgttgagga tcgtgagctc
      121 ggacgcccag cgcgacagcg gcgcgaagtg cggcccgagg cggagctcgc cggttcgggc
      181 cacgcggttg tcgccgggca aggtgacgat gccgtcgacc tcgctcgcgg tcttgggatc
      241 ggtcgtgtag cgcgtgtcgt ggccgcccga caggagcatc aggacccagt agcgcgggcg
      301 tgacgcgccg gccgcgcggc cggaaggttg cgaatcacgg cagccgaggg acgttcccgt
      361 cagccaggcc gctgtaccca aggcaagctt gacgatgtcc cgtcgggtcg gcactctcga
      421 agcccagcat atcacgttcg cgcggcggcc ctcccccccg agagcagatc accgagatca
      481 cagcgcagcc cacgtgatgc caaagacata cacgttcgcg ttctgcgtgt tggagccggt
      541 cacgaacagc cgcgctcctg cgttgatccc cagagcgttg gtgagctgat agcccacccc
      601 gccgccgacg agcaggatgg tctcggcgag gatggggtcg tgatagagct ggtcgttcat
      661 cggcagcatg gtgaactcgc tgaagttcat cccgccatgc gtcacgcggc cgttgacgtc
      721 gacgtggaga tcgagccgct gatcgagcag cttgtggccg accgtgaagg tgaagtcgct
      781 ccggttctgg ccgaactgcg cggtctccat cgtgcggtcg tacttctcga cgatgctgaa
      841 ctcgtagagc gcgtgcacgt aggtcgactc gccgaacacg cggccgaccg ccagacccag
      901 gtgcagcgcc ttgaggtgcc ggcctgccac ggcgttgccg accgtctcgt agctcgcgac
      961 cgggatcgac accgcgatgt gcggggccag cgccagcgga tcctcgagca gctggtagcg
     1021 cgccccggcg cgcaggtcgg tcagggtggc gtggaggctg ccatcgtcat agcggccgcc
     1081 gccggggtgc gccgacgccg ccgacttgac gcccgtgtac tcgagcagcg ccatgggcag
     1141 cccgacgccc accgcgaggt gctccagcgg cacgtactcg gcggcgagcg tgatctcgtg
     1201 ggtcgtgacg ccggcgtcgg cgaagtcgct gcccttgtcg acgatcacct tgtcggagat
     1261 cccgaggttg tagccgaggt tgagctcgag ggtccccttg tcttccaccc acgcctgggc
     1321 gcgcgcgtcg cgccccgccg ccaccagggc aacccaggcc gatgcgacaa caaggctgaa
     1381 actggccggg caacgtctca ttgctctccc tcgcaaactg gacaaggttg gatccacgtt
     1441 ctatcagagt ttcgggaaga tcatcacaat atggccacga cgatctgggc gcacctcacg
     1501 tgccttgcag cggtggcggc cggcggctgc tcgcccgcgc ccaggggccg gatcgttgtc
     1561 gcgatcacga tcgactggga gggcgcgtac ctgtcgccgg acgggctgga tgcgcttgac
     1621 gacgttcgcc ggagtctcgg tccggcgccg gtgacgcact tcgtctcggc ggcgtacttc
     1681 accaaggatc caccggatcc gaaggcagcc gcgacgatca cccaggaggt ccgcaagggg
     1741 gacgagctcg ccgtccacct gcacgcgtgg cgctcgctcg cccgggccag cggtatcgag
     1801 cccaggctga gtccgtcgtt cctcaccggg actgacaagc tcctggagtt cgaggatggc
     1861 gatctcgggt tcgataccga tctcgatgcg tacagcgtga ccgacctgcg tgtgctgctc
     1921 cgcacatcgc ggcggctgct ggaggggact catctcccgg tgtccaggac gttccgcgca
     1981 ggcggttacc tgggcacgcc caaggtgctg caggcgatcc aggccgaggg cttcgccgtc
     2041 gactgctcgg cgaccgacca ccgccagctc gacgaacgca aggacgcggt cctgcccagg
     2101 cggctccagg cgatctggcc cggcgtggag ccgacctcgc agccgtggtc gatcgatgcc
     2161 cccggtgatg ccccgggcgg cccgctgctc gagatgccga tcgccgcgat cgccgactac
     2221 gccaccccgg cccagatcgt ggccgtgttc gaggccgcgc atgctcggct gcgcgacgac
     2281 ccgcgccggg acgtgttcgt ggtggtcggc ttccatcagg agaccgcaca ggagttcgcc
     2341 ggacggctcc gcgaggccct ggacaggatc cgcgggcggc gcgagatcgc cgacgagctg
     2401 atgttctcca cggtcgagga cgccgcggcg cgggcccggc gcacggcggc gcgtccgccg
     2461 tcgtgatcac ggccgcgcgg ccgctcgaac aacctcgcga tatctcgtcc aggacgatcc
     2521 gggccttgca accgccggat cgcgaggcag gcccacgtac aagcgcttgc caggacgaaa
     2581 gccgctcgtt aacaatgtat gcgcacgccg tcgatgggat cgacgtgcta ggaggtgttg
     2641 atgaagtgga acttcgctgc cgggctcgtg actgcgatct tcgtggcaat gcacgcagct
     2701 ccgccagctg atgcgtgcgg tgtcaagctc accgtcaaga gctcgacgcc gcgcaaagcc
     2761 gtcgctcgca cctcgaatcc ctcggatatc ctgcttctgg gcaacccgcc gcgccggctc
     2821 gagagcgatc tttcagcggc cgggcaccgg gtcgatgtcg cgcccacggc cacggccgtc
     2881 aagcgcaagt cctatgcggt cgtgatcacc gatgccagcc tgctagacga ggcgcgatcg
     2941 agcttcccgg acgcggtcgt gatcgtccgg tcgggtgacg agaccgccga catgcgctcg
     3001 gtggagcagc aggtcgcccg caagccggtg cggaccgagg ccgcccgcag cgcggtcgcg
     3061 gcgcgcccga cccgcacccc cgttgccgcg ggtccgccgc aggatccgaa ccggcggatc
     3121 gtcgcggcca gcgagcccaa ggacacgccc gaaccggcgc caccgccccc atcgccggcg
     3181 ccggagaaag tcgcggtcac gacccccaag gtcgagacgc gcagtgtcga gccgaaagtg
     3241 acggacgtcg ctcccacgcc gaccccggaa ccgagggcca gggcagtggg caagcccggg
     3301 accatccatc atgagctgta tttcgcactc ggcagcttca agatcgatcc ccgctcgctg
     3361 gcgcgagacg tgagttggct cacgaacaac aaggatgtcc aggtggtcat cgaggggcat
     3421 gccgacccga gcggcagcca cgaaggcaac atggcactcg ggcagaagcg cgcggaagcg
     3481 gtgcgcgatt ccctggtgtc cgccggcatc gaggagtcac ggctcgaggt gatctcctac
     3541 ggtgacactc gccttcgcta cggccgggcg gacggccgga atcgccgcgc agcgatcgtc
     3601 gtgaagtagg tagctgcggc atgtcgcacg cgcgcgtggt ctgcagcgcc ctgggggtcc
     3661 ccctgctgct cgcgtcctgc ggcagccccg cgagccacag ccccgatgcg gcggccgggc
     3721 cgtgctggcc gctggacgcg atgccgggcg gccaggccga gctcggcacc ggagacatcg
     3781 acttcgagcc gatgcccgcg atgctgccca tcatcaggaa tgcgagtcag tcggacccgt
     3841 ttctccagat ccacgctcgc atccacggcc tgccgccggg caaccccgac gatatcctgg
     3901 ctccgggcaa tccgcggacc aaggtaggcg caacgatcga cgagctcggc ctgacgctcg
     3961 gggtcgagtg tccggcgagc ctggggtacg tcgcgtcgcc cgaggccggc gccttcgacc
     4021 tcgtgcactc gctcaggatc ggcctcggga ccatcccgcc cgctcaggtc gcgggcaagc
     4081 aggcccacat caccctcgag gtcgtcggct cgaatggcct ctacgcgcag gatatgaagc
     4141 tggtgacggt gacgctgatg accaccagcg cagcccgctg acggttacgg aggcgtgccc
     4201 tgcacgtcca tgagctgcgc ccagcacatc agggtcagca tgtcgatgcg gcgcttgttg
     4261 gtggtggagt tgttgggcag ccgccgcaac tggtgcacca cgccgttgcg cagcatgacc
     4321 atcttgttga acggcaggcc gcgcgccttg gcgatcgcct ggtactcggc gatggccgtg
     4381 cccggaaccg tgaactgacc tgcggtcatc ggcgcaggac acatgtgggt cggtgcgccg
     4441 gtgacgtcca ccagccaggt gatgacctgg agctggtcgc ccgccggcag gcccgggctg
     4501 ttggtgggcg tccagtgcac ggtgaagtcg gtgccggcca ccagcggccc gttctcctcg
     4561 tgggtggggt tgctgacgtt gaacgtctgg gccaggtaca ggccaccgtc gaacgtcgtg
     4621 gccgggatcg gcgtggtcgc gcccgtgggg gagccgccga acttgaccgc atagggggaa
     4681 tcgggcttga ggaagctctc ggcgatgggg ttgatcgcct ggtagaagat gtcgtgcggc
     4741 cggccgaaga cgtcggtccc cttcggctgc ttgtcgaccg tgatcgtcac gtcggtcccg
     4801 gcggtgttct tgccggtgag ggtgaccgtg ccgacgtcca gatcggtgtg cggcgtcccg
     4861 atgtaccctg gccagccctt ggtcgccacc aggttgttgc actggcccgg cgtcggcagc
     4921 aggttggcct cgggcgtctg gtcgttcatg aagaacgccg acacccggtt cagcgtggtc
     4981 accccggcgg ggagcttgaa aaacgcctgc agctcggtgt cgaggtacat gtactcgagg
     5041 acgatctggc cgccctcggg atcggtgagc atcgagtcgt cgcactggtg accgacgcag
     5101 ttaccgccgt cggtcgtcga gacatcgcca ttgccacaag cagacagagc tgctgcagac
     5161 aggaacatcg ggccgatatt gcgtgaaagc attgtattcc cccgcggccc gaatctacat
     5221 gagctcgccg aggacgagca actatttccc cgtatgaaag attgaaaaca gcacctgctg
     5281 ttggatctgt gtgacagaca gcatggcgag tcggggacga ctgacgtcag gccggacatc
     5341 cgtcgttgcc agtccgcccg ggctcggcac cgtcggcggc gacgttctgg aaccagcgct
     5401 ccgaaggcct cgaaagactt cctgatccgg cgaggcgttt ggcggcacgc gtcgttgtgc
     5461 ggcgacaccc cgcacgggct ggacagctcg gcttgccctc ccggaccgtt cacaaaatgc
     5521 aataggtgcg tgggcccacg cagctcggcc ctgtgccgga tgtcactgcg gcacgagaaa
     5581 caccgcggac tcgtggctcg agggactcgc tcggcaccac gagcgtgctg tctccggacc
     5641 catggctgcg ctggatccga tcgagcaggt cggtgcgccc gaaccgctcc tcgagccctg
     5701 tcgtgctcca cagcgaagga gcttcacgcc ggcggcccgg gacggcggtc gtcgcgtatc
     5761 acgcagtttc gattccggac cagcccggga agatgggata gttgcttcga tgaccacgaa
     5821 gcaccgtccg gcgcaccctc gctgtgcggt ggctcacgga agggcatctg aggtttactc
     5881 atgaccacgg ctcccatcgc ctccaagccg ctgtcccaga cacggaacct ggacaagttc
     5941 gcgttcgaga agtcgacctg gggcggtgtc acctacctca cgctgcacgg cgtgctcgat
     6001 caggccttcg acgggcgcaa gctcgccgac ctggtccgta cccagaaggt ggtcgtgagc
     6061 ctgcgcaacg tccgccggtt cgcgtcgtgg gggatgtcgg agtggatgga gttcttgcag
     6121 gtcaacgcgg cgcgcgatct gtacctcgtc gagtgctcga cctacgcggt gagccagatc
     6181 aacctcgtga acgggctgct ggggcacgcc aagctggtca gcttctatgc gtcgtaccgc
     6241 tgcgccagct gcagcgaggc gcacgagagc gtgttcatcg tcccgcgcga ccgcgagatc
     6301 atccgcgagt tacccggcaa caccgaggag tgctcgacct gcggcggccg cgcgcggctc
     6361 gaggagtacc ccgccgcgtt cttcgacacg atcaccgacc gcccgtcgtt cgacatcgac
     6421 gacgaggtcc tggcgttctt tcgctcgcag ctccagtact ggctggctcc cgatctcagc
     6481 cagttccgcg cgcaccggcg gctggacaag ggctacacct atctccggct gtcgggggac
     6541 ctggcggcgc tgcccggcga gcgcctgctg gcggcttccg agggcacgac cgtggtggac
     6601 ctcgccggcg ccgtgttcga gcccggccag ctgaccgcct ggcgaacgta cgtgcgcggc
     6661 gcgctggcca agctcaggtc gctgcagctg ctcgactgcc cgctcggatt cctcgaggac
     6721 gccgtggcgc cggaggacct gcgcgacaag ctcaagatcc ggacgttcgg gctatcctac
     6781 gactgcctgc gctgcgagac ggccgtgacc cggatggtcg gcgtcgccga gaacctcgag
     6841 ctgctggtcg acggcgtcgc gccgcccgcg cagtgcacga cgtgcggatc gcagctcgtg
     6901 gcgatcccga ccgcccgcca ggccgtggtc atgcgcagcc tgccggcgcg ggatcgcgat
     6961 cgtgcgctcg acaagttcct cgccgcggcc cgcgccgagc cgctggacaa gctcgagaac
     7021 tgtctcgccg cgctgcccgc gaagctgccc accgcgcccg cgcggacctc gagcgcggtg
     7081 ttcgtggcgc tggcgctggc gctgctggtg atcggcggcc tcggtgccat ggtggttgcc
     7141 ctgtggcgac agcgcggaga gccggcccgg gccgccatcg ccccggcgcc ggccgccgcg
     7201 cccaagccgc cgccgacctt cgagcggccg gcgtggatca tgtccgacat cccatcgtcg
     7261 gcctactgct acgacatgat caaccggctg atgtgcatcg gcgtatcgtc ctaccggccg
     7321 acgcgcgacg aggcggtggg cgaggcgaac gacgcggcgc tcgacgagct ggtcagcgcg
     7381 atcggcctca agatcagcga cccgttcttc cgcgagaccg tgatgtccag ctacgccggg
     7441 ccccgcgcca aggcactgtc ggcgctgcag gccaccgacc tggagcagac cagccccgcg
     7501 tacgccgctg ccgccgacgc ggtcgccaag gcgcgccggc gcgtggccga gatcctccgc
     7561 gcctcgggcg gtgccgccgt gccggcccag cgctccgact ggtactggga ggagtacgcc
     7621 gccaagaagg gcgggaccga ggccctggtg ttcattcgct acgacgtcac gctcgacgcg
     7681 atcaaggcgc tcgtcgagaa gtactcggcc accacgaccg tcctgggcag caccgcgatg
     7741 accgcgttcc ccgcgctggc gtggcaatcc gccgacttca ccggcggcgc gatgctgacc
     7801 cgggtcgcgc acccgctgtc ggacgccggc atcgccgggg cgaacgtggt cctggccgtg
     7861 ggcgaccagc gggtgaggga cgcgacgagc ttcgcgcggc ggatcgagga gtggaagcag
     7921 gggacggggg agctccagct gacggtgacg agcggcgaag gccccgcgcg cgtggtcgac
     7981 gtcaagcgcc gctgacgcgc cccgtcagct gggccggacc agcatgtaca ttccgaccgc
     8041 gagcaggatg gcgaccgcga gtgtgaccag cagcttcacg acggagaacg aggggcgcac
     8101 ggaggggcga accggcggta tcaggctcgg cagggacacc ggcggcacga cctgcggtct
     8161 ggacacgggc acgggcaccg ggctcggcgg aggcagcgca acggggcgcg agggctccgg
     8221 gactctcggt gtcggcgtgg gagggaggtc gagcgccgcg ctcgccgggg gcacgccgtg
     8281 cagcgtcagg gaggacgatg gcaacgagct ggtcggcttg ctgtcgtacg cgccaatgaa
     8341 ggtgctggtg acctggatcg gctcggcccc gcctgccgtc tcgacgggga acagctgcga
     8401 gaggacgtag gcgatgcccg gcgccgagcc cgacagcccg tactgccggg agatgtcggc
     8461 cagctcggcg gccacgtcct gcccgcgctg cggccggtcg tcggggttgg gcgccagcag
     8521 ccggttcacc agcgccacca
//
LOCUS       DASVOR010000974         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_477539,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000974 DASVOR010000000
VERSION     DASVOR010000974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477539"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..114
                     /locus_tag="VF516_18875"
     CDS             <1..114
                     /locus_tag="VF516_18875"
                     /inference="COORDINATES: protein motif:HMM:NF012783.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEX8109806.1"
                     /translation="QPVERIKHDTERDNFMSAAEARDYGLIDEVLTTLKKS"
     gene            complement(134..499)
                     /locus_tag="VF516_18880"
     CDS             complement(134..499)
                     /locus_tag="VF516_18880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109807.1"
                     /translation="MLIGVAAVGCGGGGDSASPRVACEDIEANVCERFYACFTPAELA
                     AASFPADEAACVSQMQAMKGCANQTTANACVGNQHYHPDQAATCANQISGLTCGQIRD
                     QSANLDADAPACDKICAVD"
     gene            676..>1782
                     /locus_tag="VF516_18885"
     CDS             676..>1782
                     /locus_tag="VF516_18885"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8109808.1"
                     /translation="MPALYDVLIVGGGPAGSTCARVLAAGGARVAVVDRAEFPRVKLC
                     AGWLSPGIWDVLQLTPASYPRGLWEWNTCHVHYRGKDHAIPCHGWFIRRYEFDDFLLH
                     TSGADLHLGTNVKQIERDASGQWAIGPLRARYLIGAGGTHCPVARMLAPTRPRRAVGV
                     QELELQLDAISVARTRLGKDGEPELVLFDDIGGYGWNVPKSDWINVGCGTLDATAARG
                     AWRHTHDHLRAAGHLPPGAEPGLEHIKGHSYFLFEPVHLDAAYRDQAFLVGDSLGLAH
                     PITAEGILPATLSGRHAAEAILAGDPASYPARLRRDPILTDYRRVHAALATVQKLRDR
                     ISRWTGLASLIDRTTTAGAGTAAPATTGAPSNGAA"
BASE COUNT          254 a          699 c          582 g          247 t
ORIGIN      
        1 cagccggtcg agcgcatcaa gcacgacacc gagcgcgaca acttcatgtc ggcggcggag
       61 gccagggact acggcttgat cgacgaggtg ctcaccaccc tgaagaagtc gtagccggcg
      121 acacggccac cgctcagtcg accgcgcaga tcttgtcgca cgcgggcgcg tccgcgtcga
      181 ggttcgcgct ctggtcgcgg atctggccgc atgtcagtcc ggagatctgg ttcgcgcacg
      241 tggcggcctg gtccggatga tagtgctggt tgccgacgca ggcgttggcg gtcgtctggt
      301 tcgcgcagcc cttcatcgcc tgcatctgcg acacgcacgc ggcctcgtcg gccgggaagc
      361 tggccgccgc cagctccgcc ggcgtgaagc acgcatagaa ccgctcgcag acgttcgcct
      421 cgatgtcctc gcacgccacg cgcggcgacg cactgtcgcc gccgccgccg cagcccaccg
      481 ccgccactcc gatcagcatc aagaatcctg caatcgtccg aactggcatc tgggtctcct
      541 cgggccgttg cggcccccgg ggacccagga gacggatacc ggcgattgtc gcgaagaaat
      601 cgcgccaccc gctggcggat cgtggccaac tcgtggcacg tcaggtgcac agcgggaggt
      661 cacgtgcccg gaaccttgcc agcgctctac gacgtcctga tcgtcggcgg cggccccgcc
      721 ggcagcacct gcgcccgcgt cctcgcggcc ggcggcgccc gggtcgcggt cgtcgatcgc
      781 gccgagtttc cgcgcgtcaa gctgtgcgcc ggctggctgt ccccggggat ctgggacgtc
      841 ctccagctca ctcccgcgtc gtatccgcgc ggcctgtggg agtggaacac ctgccatgtc
      901 cactaccgcg gcaaggacca cgcgatcccg tgccacggct ggttcatccg ccgctacgag
      961 ttcgacgact tcctgctgca cacgagcggc gccgacctcc acctcggcac aaacgtcaag
     1021 cagatcgagc gcgacgccag cggccagtgg gcgatcggcc ccttgcgcgc ccgctacctg
     1081 atcggcgccg gcggcaccca ctgcccggtc gcccgcatgc tcgccccgac gcggcccaga
     1141 cgcgcggtcg gcgtccagga gctcgagctc cagctcgacg cgatctcggt cgcgcgcacc
     1201 cggctcggca aggacggcga gcccgagctg gtcctgttcg acgatatcgg cggctacggc
     1261 tggaacgtgc cgaagtccga ctggatcaac gtcggctgcg gcacgctcga cgccaccgcc
     1321 gcgcgcggcg cctggcgcca cacccacgat cacctgcgcg ccgcgggcca cctcccgccc
     1381 ggtgccgagc cggggctcga gcacatcaag ggccactcct acttcctgtt cgagccggtc
     1441 cacctcgacg ccgcctaccg cgatcaggcg ttcctcgtcg gcgactcgct cggcctcgcc
     1501 cacccgatca ccgccgaggg catcctcccc gccacgctgt ccggccgcca cgccgccgag
     1561 gcgatcctcg ccggcgatcc cgcgagctac ccggcccggc tgcgccgcga tccgatcctg
     1621 accgactacc gccgcgtcca cgccgcgctc gcgaccgtgc agaagctgcg cgaccggatc
     1681 agccggtgga ccggcctggc gagcctgatc gatcggacca cgaccgccgg tgccgggacc
     1741 gcggccccgg cgacgaccgg cgccccgagc aatggcgcgg cg
//
LOCUS       DASVOR010000975         3696 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_447979,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000975 DASVOR010000000
VERSION     DASVOR010000975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3696
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_447979"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(200..1963)
                     /locus_tag="VF516_18890"
     CDS             complement(200..1963)
                     /locus_tag="VF516_18890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8109809.1"
                     /translation="MSIDRRSTPTGTEASPGTSDSTTLGSFGKRTLTGELPSAAMSGV
                     AGKHTLTGGLVQRKERPGSGEVPANAQAIASAGVQDGGGALPHGHRIQALFGRHDISG
                     ISAHVSGPAAEAADQLGASAYATGHHVAFASAPDLHTAAHEAAHVVQQRDGVALKGGV
                     GAVGDPYERHADAVADRVVAGQSTEDLLDGATTGAASAAVQRRELPDTTPLGSSADWH
                     AGDRARLGGSFETANLFNLAANDHAQYTRIEERRDFYRWFYNYTSAMGCTTRWALAAS
                     LVANGANQVAHMDPVMEGAAQLVGTVNDELQGMMRIGNQVIFDNVFPKLNRLLIRARS
                     GPVTGAAALRWDMDTLAEEQTLIQPLYTSVSPATIAALNNVARQQGWAGIGAWATSGA
                     RVAPGPYNRGGDMPAFGTGAPGASITHINDRWNYGMQVGAQFAPTPTGFVPGTPMPAV
                     PGSYTSGAELAAVDTRHNLHMLEACLDSPRDADPTRILALLRALTPTEQAQFNTDLSP
                     DGTQYSYQLGECSFDVAALTGAIIERPTMTSSIPTIVGAYTGGPGPSTFLGRYATRVA
                     TLRSLSRSTLSLPPPTLGAPF"
     gene            2126..3253
                     /locus_tag="VF516_18895"
     CDS             2126..3253
                     /locus_tag="VF516_18895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109810.1"
                     /translation="MTPPARPADPAVSQIADAAEDATAEAAVWSGEHAVVVGSAGATG
                     PTPTGANPIFADGARYHGWIRAIDAHGGSGWMRRFDTGREVHVRAAAVVGDEVVIAGE
                     QRDGDARAYTGWVARIAPGGVERWRLEHLGAAGATGLQAVAVRGDGSVAAGGMQRGKG
                     WLVAIDPHGKLRWDHDLADLDEATAVMPIGDDFVMAGITGRTTTSAGTSRLTRIDATG
                     AARWSVTVPERGPGELFALAPLGDGGVAVGQAPGAGGRDGAWIVRFDAGGAIRASQVL
                     PAKGSDSARAVAATADGGFVVAGSSFEAPQDRRAIVWRFDGANQLVWRQAYGSGAFAR
                     GVAGAPDGGAVVVGATQPAGAKLRSWIAGIDRGGTQRWTAP"
BASE COUNT          493 a         1240 c         1421 g          542 t
ORIGIN      
        1 cgccggccgc gtcggcggat ccaggccggc cctcgagccc ctgagccgag cagcgccttc
       61 cgcgatgcag ccgcgacgcc gccccgctgt gcaccgcgac cgcgcagccc ggcgcagcca
      121 ggtccccgcc aggcgtacgc caccgcgctc gcctgcagag catggattgt tcaccagcgg
      181 gccacccggg acccgggcgt cagaacggag cgcccagcgt cggtggcggc aggctcagcg
      241 tgctccgcga gaggctgcgc agcgtggcaa cgcgggtcgc gtaccggccg aggaacgtcg
      301 atgggccagg tcccccggta taggcgccga cgatggtcgg gatgctcgac gtcatcgtcg
      361 gccgctcgat gatcgcgccg gtgagtgcgg cgacgtcgaa cgagcactcc cccagttgat
      421 aggagtactg cgtgccgtcg gggctcaggt cggtattgaa ctgggcctgc tcggtcgggg
      481 tcagcgcgcg cagcaaagcg aggatgcggg tcgggtcggc gtcgcgcggt gagtcgaggc
      541 aggcctccag catgtgcagg ttatggcggg tgtcgaccgc ggcgagctcg gcgccgctgg
      601 tgtagctgcc cggcaccgcg ggcatcggcg tcccggggac gaagccggtg ggcgtcgggg
      661 cgaactgcgc gccgacctgc atgccatagt tccagcggtc gttgatgtgg gtgatcgacg
      721 cgccgggagc gccggtcccg aaggccggca tgtcgccccc gcggttgtac gggcccggcg
      781 ccacgcgggc gccgctggtc gcccatgcgc cgatccccgc ccagccctgc tggcgtgcga
      841 cgttgttgag cgcggcgatc gtcgccggcg atacgctcgt gtacagcggc tggatcagcg
      901 tctgctcctc ggccagcgtg tccatgtccc acctgagcgc ggcggcaccg gtgaccggac
      961 cggaacgggc gcggatcagc aggcgattca gcttgggaaa cacgttgtcg aagatcacct
     1021 gattaccgat ccgcatcatg ccctgcagct cgtcgttgac ggttccgacg agctgtgccg
     1081 cgccctccat gaccggatcc atgtgcgcga cctggtttgc gccgttggct accagcgatg
     1141 cggcgagcgc ccagcgcgtg gtgcagccca tcgccgaggt gtagttgtag aaccagcggt
     1201 agaagtcgcg gcgctcctcg atccgcgtgt actgcgcgtg atcgttggcg gcgaggttga
     1261 acaggttggc ggtctcgaag ctgccgccga ggcgggcgcg atcgccggcg tgccagtcgg
     1321 ccgacgagcc cagcggggtc gtgtcgggga gctcgcggcg ctggaccgct gcgctggccg
     1381 ctccggtcgt ggcgccgtcg agcagatcct cggtggactg gccggcgacg acccgatccg
     1441 cgacggcgtc ggcgtggcgc tcgtacggat cgcccaccgc gccgacgcct cccttgagag
     1501 cgacgccgtc gcgctgctgc accacgtgcg cggcctcgtg cgcggcggtg tggaggtcgg
     1561 gagcgctcgc gaacgcgacg tgatgaccgg tcgcgtacgc gctggcgccg agctgatcgg
     1621 ccgcctccgc agcgggcccg ctgacgtgcg ccgagatgcc gctgatgtcg tgtctgccga
     1681 acagcgcctg gatccgatgg ccatgcggaa gcgctccacc accgtcctgc acacccgcgc
     1741 tggcgatcgc ctgggcattc gctggaacct cgccggagcc gggtcgttcc ttgcgctgga
     1801 ccagcccgcc ggtgagcgtg tgcttgccgg ccacgccgga cattgctgcc gacgggagct
     1861 cgccggtgag cgtccgcttg ccgaagctgc ccaaggtagt tgaatcactg gtgcctggag
     1921 acgcctcggt gccggtcggt gtggagcgcc ggtcgattga catgcgatac attggtacgt
     1981 gagagttgtc ggcaagattc ctgtgcgtgg ctcacgcaat gcaccggcgc ggtcgatacg
     2041 ctggcgcggt gccaacgctc gcagccgatc gctgcagctg ctgagccttg cactggcgtg
     2101 caactcccaa ccgatgaaca acgccatgac gccacccgcc cggcctgccg accccgctgt
     2161 ttcccagatc gcggatgccg cggaggatgc gaccgccgag gcggcggtgt ggtcgggcga
     2221 gcatgcggtg gtggtgggga gcgcaggcgc aaccgggcca acgccgacgg gcgccaaccc
     2281 gatcttcgcc gacggcgcgc gctaccacgg atggatccgc gcgatcgacg cgcacggcgg
     2341 ctcaggctgg atgcgccgct tcgacaccgg gcgcgaggtg cacgtccggg ccgcggcggt
     2401 ggtcggcgac gaggtcgtga tcgccggcga gcagcgcgac ggcgacgccc gcgcgtacac
     2461 gggctgggtc gcgcggatcg cgcccggtgg cgtcgagcgc tggcggctcg agcacctggg
     2521 agcggcaggc gcgaccggcc tgcaggccgt cgcagtgcgc ggcgacggca gcgtcgccgc
     2581 gggcggaatg cagcgaggta agggatggct ggtcgcgatc gacccgcatg gcaagctgcg
     2641 ctgggaccac gacctcgcag acctcgatga agcgaccgcg gtgatgccca tcggtgatga
     2701 cttcgtgatg gcgggcatca ccgggcgcac cacgaccagt gcgggtacca gccggctgac
     2761 ccggatcgat gcgaccggcg cggcgcgctg gagtgtcacg gtgccggagc gtgggccggg
     2821 cgagttgttc gcgctcgcgc cgctgggcga tggcggggtc gcggttggcc aggcaccggg
     2881 cgcaggtgga cgcgatggcg cgtggatcgt gcgattcgac gccggcggcg cgatccgcgc
     2941 gagccaggtg ctgccggcga agggcagcga ttccgcgcgc gccgtggcgg cgaccgcgga
     3001 cggcggcttc gtcgtcgcgg gcagctcgtt cgaggcgccg caggaccggc gggccatcgt
     3061 gtggcgattc gatggcgcga atcagctggt gtggcgccag gcgtacggca gcggggcgtt
     3121 tgctcgtggt gtggcgggcg cccccgatgg cggcgcggtc gtggtgggcg cgacacagcc
     3181 agcgggtgcc aagctccggt cctggatcgc cggcatcgat cggggcggta cacagcgctg
     3241 gaccgcaccg taggccgcaa ccgctagcaa ctcgggaccc ctggcacatg gcaagttttc
     3301 cgccgaactc ggaccgcgcg gcgtgcgtcg agaaggactt catgcgggac ctcatgtggg
     3361 gcctatgtaa gggcgggccg tgccccaaga gcaaccgacg ccgatggacg tatcgcggcc
     3421 ggtgtcctgc cccgccgcgc ggttgcgtgt cgcatggcat ccgcacggcg ccggtcatgg
     3481 ctccgctgtg ggcgggccgg gcagccggtg catgactggt gcggtgcggc gtgcggagtg
     3541 gcagcccgga gtgacagctg gatgtgcagc ggtcgccggc ctggcgcatg atacccagga
     3601 ccatggctcc ccccgacgct ccgccgatcc tggtgctcgg cgatccgcgg ctgcgcgttc
     3661 cgtgcgccgc ggtcgaggcg gtcgacgccg cggagc
//
LOCUS       DASVOR010000976         2605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_39128,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000976 DASVOR010000000
VERSION     DASVOR010000976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39128"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="VF516_18900"
     CDS             <1..1071
                     /locus_tag="VF516_18900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109811.1"
                     /translation="LWWALGILLALGVHDYLPPDDRTAAATLRARLIGVGVAIGVATA
                     SRFVNGLLGPLCALIVVVQAPPRWRRDTAIWGAWRMPLAAIATVYAIWPRLWAHPIAS
                     LVESFHKLDAAHAPEPWLGGITNHPGPLYFAIYLAATLPLGVAIGVVAGAVRLIRRPD
                     KSALITAACLIVPLGVALSPVRQDGVRYAMPCLLALALIAAAGFDQLAMWARHRLAFA
                     AIAAAAVAYLAVTLWRVHPYYLDYFGEQVGGAGQVAERGWFETAWWGEGVDRAVDYVN
                     AHAAPGARVDRRCIEPVHLAWFRDDLWAAMTDDPARADWIVSYSPASHPCPVPRDARA
                     VFEVTGGGVVFAIVYQRAASGR"
     gene            1137..2012
                     /locus_tag="VF516_18905"
     CDS             1137..2012
                     /locus_tag="VF516_18905"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8109812.1"
                     /translation="MLGALSTFALTTATMIAFAANSLLSRAALRGGAIDAMSFTAIRL
                     LSGTLVLVAITQARRSAADTGRHGSWSAAIALGGYAIAFSLAYLRLGAGAGALLLFGT
                     TQLTMITGGLVRGERPSLRQWVGLAVAAVGMVVINLPSLDAPPPSGAALMIAAGVGWG
                     LYSLSGRGAKRPIRATAGNFVRCLPFTAAFGVIAIAATAEVTTRGVVLAAVCGTLTTG
                     LGYCVWYAVLPSLGAARGAIVQLSVPVIAAVGAIVLLHESLGLHLAIGGAIILGGLAL
                     ALWQRRPVPAAAAAR"
     gene            2156..>2605
                     /locus_tag="VF516_18910"
     CDS             2156..>2605
                     /locus_tag="VF516_18910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109813.1"
                     /translation="MDFVFMLTRADQTVPDCLEVLDDIRPLGLTHIGFKDVGVTPEVL
                     AELARRIKDVGATSYMEVVSTSREACLRSARVARDIGVDRLLGGTQVEEVLAILAGSA
                     VRYLPFPGRPFEHPTKLAGTPAQVEDDCRRFQAMGCAGVDLLAYRATE"
BASE COUNT          273 a          960 c          978 g          394 t
ORIGIN      
        1 ctgtggtggg cgctcgggat cctgctcgcg ctcggcgtcc acgactacct gccgcccgac
       61 gaccgcacgg ccgccgccac gctgcgcgcc cggctgatcg gggtcggcgt cgcgatcggc
      121 gtcgcgaccg cgtcgcggtt cgtcaacgga ctgctcggcc cgctgtgcgc gttgatcgtg
      181 gtcgtccagg cgccgccgcg ctggcgccgc gacaccgcga tctggggcgc gtggcggatg
      241 ccgctcgccg cgatcgccac ggtctacgcg atctggcccc ggctgtgggc gcacccgatc
      301 gcgagcctgg tcgagtcgtt tcacaagctc gacgccgccc acgcgccgga gccctggctc
      361 ggtggcatca ccaaccatcc cggcccgctg tacttcgcga tctacctcgc cgcgaccctg
      421 ccgctcgggg tggcgatcgg cgtcgtcgcc ggcgcggtgc ggctgatccg ccggccggac
      481 aaaagcgcgc tgatcaccgc cgcgtgcctg atcgtcccgc tcggcgtcgc gctgtcgccg
      541 gtccgtcagg acggggtgcg ctacgcgatg ccgtgcctgc tcgcgctcgc gctgatcgcc
      601 gccgcgggct tcgatcagct cgcgatgtgg gcgcggcacc gcctggcgtt cgcggcgatc
      661 gcggccgcgg cggtcgccta cctcgcggtc acgctgtggc gcgtccatcc gtactacctc
      721 gactacttcg gcgagcaggt cggcggcgcc ggccaggtcg cggagcgcgg ctggttcgag
      781 accgcgtggt ggggcgaggg cgtcgaccgc gcggtcgact acgtcaacgc gcacgccgcg
      841 cccggcgcgc gggtcgatcg ccggtgcatc gagccggttc acctcgcgtg gttccgcgac
      901 gacctgtggg cggcgatgac cgacgacccg gcgcgcgcgg actggatcgt gagctactcg
      961 ccggcgagcc atccgtgccc ggtgccgcgc gacgcgcgcg cggtgttcga ggtcaccggc
     1021 ggcggcgtcg tgttcgcgat cgtgtaccag cgcgccgcgt cgggccgctg agggcgcccg
     1081 cgtgtgaacg agtgttgcgc ccgttccggg gccgcggatc tgcgtatgat gccggcgtgc
     1141 tgggcgcgct gtcgacgttc gcgctgacga ccgcgacgat gatcgcgttc gcggccaact
     1201 cgctgctgag ccgcgccgcg ctgcgcggcg gagcgatcga cgcgatgagc ttcacggcga
     1261 tccggctgct cagcggcacg ctggtgctgg tcgcgatcac gcaggcgcgg cgcagcgccg
     1321 ccgacaccgg ccgtcacggc agctggagcg ccgcgatcgc gctcggcggc tacgcgatcg
     1381 cgttctcgct cgcctacctc cggctgggcg ccggggccgg cgcgctgctg ctgttcggca
     1441 cgacgcagct gacgatgatc accggcggcc tggtgcgcgg cgagcgccca tcgctgcgcc
     1501 agtgggtcgg gctggcggtg gccgcggtgg ggatggtggt gatcaacctg ccgagcctcg
     1561 acgcaccgcc gccgagcggc gccgcgctga tgatcgccgc cggcgtcggc tggggcctgt
     1621 actcgctgag cggccgcggg gcgaagcgac cgatccgcgc gaccgccggc aacttcgtgc
     1681 gctgcctgcc gttcaccgcg gcgttcggcg tgatcgcgat cgccgcgacc gccgaggtga
     1741 cgacgcgcgg cgtcgtgctc gcggcggtgt gcggcaccct caccacgggc ctgggctact
     1801 gcgtgtggta cgccgtgctg ccgtcgctcg gcgccgcgcg gggcgcgatc gtccagctgt
     1861 cggtgccggt gatcgcggcg gtcggcgcga tcgtcctgct ccacgagtcg ctcgggctcc
     1921 atctcgcgat cggcggtgcg atcatcctcg gcggcctcgc gctcgcgctg tggcagcgcc
     1981 gcccggtgcc ggccgccgcg gcggcccgct gaccttgccg cgtacgtgac tgcggggtga
     2041 cgtgcgcgtg ccggcgcgtc cggcggcgat cgatccacac ccggccacac caggtgcggc
     2101 agatgccatc gcgccggcgt tgcaatcgcc tggccggcgt gctcactcaa cggtcatgga
     2161 cttcgtcttc atgctgacgc gcgcggacca gacggtcccc gactgtctgg aggtgctcga
     2221 cgacatccgg ccgctcggct tgacccacat cgggttcaag gacgtcggcg tgacgccgga
     2281 ggtgctcgcc gagctcgccc ggcggatcaa ggacgtgggc gcgaccagct acatggaggt
     2341 cgtcagcacg tcgcgcgagg cctgcctgcg ctcggcgcgg gtcgcgcgcg acatcggcgt
     2401 cgatcgcctg ctcggcggca cgcaggtcga ggaggtgctc gcgatcctcg ccggcagcgc
     2461 ggtccgctac ctgccgttcc ccggccggcc gttcgagcat ccgaccaagc tcgccggcac
     2521 gcccgcgcag gtcgaggacg actgccggcg gttccaggcg atgggctgcg ccggcgtcga
     2581 cctgctcgcc taccgggcga ccgag
//
LOCUS       DASVOR010000977         5022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_214481,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000977 DASVOR010000000
VERSION     DASVOR010000977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214481"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VF516_18915"
     CDS             <1..208
                     /locus_tag="VF516_18915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109814.1"
                     /translation="DGLSDLVDDWMIREIMTSGDSLEVVAENLIRAANNNGGTDNITV
                     VLAQTFVDPLWVPAHAYPPQMQRY"
     gene            complement(261..965)
                     /locus_tag="VF516_18920"
     CDS             complement(261..965)
                     /locus_tag="VF516_18920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109815.1"
                     /translation="MASAICPNCKILLVEGDTNSFANLAAAVNTAASLGAHVISNSYG
                     GGEAGTAATEAAYNHPGIAVTASSGDSGFGVEFPASSPHVVAVGGTSLRTAANTRGWT
                     ETVWSGAGSGCSTVYAKPSWQTDTGCAMRTVADVSAVADPNTGVAVFAPSSRSRSAFL
                     VFGGTSVAAPLIGAVYANNGGTVNFGSDPYAHTSALFDVTSGSNGTCSPAYLCTGGAG
                     FDGPTGLGTPNGTAAF"
     gene            complement(993..1379)
                     /locus_tag="VF516_18925"
     CDS             complement(993..1379)
                     /locus_tag="VF516_18925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109816.1"
                     /translation="MSADDEVTTTMTSDEIASASHARVCAAAQLGEASCHARVLVGPD
                     GKPVPNATPAGLGPAQLVNAYKITGGGSSAITIAIVDAFGYPNAESDLATYRSTFGLS
                     ACTTANGCFRKVDQRGGTSFPRLDVG"
     gene            complement(1584..1943)
                     /locus_tag="VF516_18930"
     CDS             complement(1584..1943)
                     /locus_tag="VF516_18930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109817.1"
                     /translation="MYPRPSWQHDTGCSRRTIADVSAVADPNTGVAVFGPSSSGTSAF
                     LVFGGTSVAAPLIAAVYAVNGGTVTFGSDPYAHTSSLFDVTSGSNGSCSSSYLCTART
                     GYDGPTGLGTPNGAAAF"
     gene            complement(2129..3256)
                     /locus_tag="VF516_18935"
     CDS             complement(2129..3256)
                     /locus_tag="VF516_18935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX8109818.1"
                     /translation="MPGDQFFNVAMTQDEITSASSAVICPDTPDGSPYCHAFGLVDAS
                     GHLITNPAPAGYSPSQLRSAYKITGTGASTTLIAVITAFDYATAEADLATYRAQFGLP
                     ACTSSTGCFRKVDQSGGTSYPGPNLNWNQEAALDLEMVSAMCQKCKIVLVEAAAPTFA
                     DLAAAVNEAAKLGAHVITNSYGGDEAGTQTYEPAYNHPGVAIVASSGDAGYFGGIQFP
                     SSSPHVIAVGGTSLTTAANTRGWTETAWDGGGSGCSTVYTKPSWQHDTGCSKRMLNDV
                     AAVADPNTGVAVFGPDSTGVSNWIVLGGTSVAAPLIGGIYGVNGGSVSYGSNPYSCLA
                     SLFDVTTGSTSAFCTPTYACTAARGYDGPTGLGTPNGSSAF"
     gene            complement(3556..4728)
                     /locus_tag="VF516_18940"
     CDS             complement(3556..4728)
                     /locus_tag="VF516_18940"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX8109819.1"
                     /translation="MRSLPFVVATFTSACATPDDPSFHVAMTQDEVVTVNVAQLCTPA
                     TSGGPYCHAHGQVDSNGRLIKHSAPAGYSPSDLRGAYKITSGSPSTLIAVVDAYDYAT
                     AEADLATYRSQFGLPACTSASGCFRKVDQNGGTSFPGPNISWNQEAALDLEMVSAMCP
                     GCKIVLVEATAATFGDLAAATNEAAALGAHVISNSYGGYEGGTEIYEAAYNHPGVAVV
                     ASSGDSGYFGGIEFPAASPHVIAVGGTSLTPTNGQRHWAETAWDGGGSGCSAVYPKPS
                     WQHDTGCSMRMLNDVSAVADPNTGVAVFGPDSTGVPSWIVLGGTSVAAPLIAGIYGNN
                     GGSVNYGSNPYSNPGALFDVTTGSTSAFCDPTYACTAGPGYDGPTGLGTPNGANAF"
BASE COUNT          799 a         1684 c         1728 g          811 t
ORIGIN      
        1 cgacggcctg tcggatctgg tcgacgactg gatgatccgg gagatcatga cctcgggcga
       61 ctcgctggag gtcgtcgccg agaacctgat ccgcgccgcc aacaacaacg gcggcaccga
      121 caacatcacc gtggtgctgg cgcagacctt cgtcgacccg ctgtgggtgc cggcccacgc
      181 ctacccgccc cagatgcagc gctactgatc ggcggcggca cagaccgagg gggccgcctg
      241 acgatcacga cgcctgacga tcagaacgcg gccgtgccgt tgggcgtgcc caggccggtc
      301 gggccgtcga agccggcccc gccggtgcac aggtaggccg ggctgcaggt gccgttgctg
      361 cccgaggtca catcgaacag cgcgctggtg tgagcgtacg gatccgagcc gaagttgacc
      421 gtgccgccgt tgttggcgta gaccgcgccg atcagcggcg ccgcgacgct ggtgccgccg
      481 aacaccagga acgccgacct gctgcgcgac gacggagcga acaccgccac gccggtgttg
      541 ggatcggcga ccgccgagac gtcggccacg gtccgcatcg cgcagccggt gtcggtctgc
      601 cacgacggct tggcgtacac ggtgctgcag ccgctgcccg cgccgctcca caccgtctcg
      661 gtccagccgc gggtgttggc ggcggtgcgc agcgacgtgc cgccgaccgc gacgacgtgc
      721 ggcgacgacg ccgggaactc gacgccgaat ccggagtcgc ccgagctcgc ggtgaccgcg
      781 atgccggggt ggttgtacgc cgcctcggtc gcggccgtcc ccgcctcgcc gccgccgtag
      841 ctgttgctga tcacgtgggc gccgaggctg gccgcggtgt tgaccgccgc ggcgaggttg
      901 gcgaacgagt tggtgtcacc ctcgaccagc aggatcttgc agttcgggca gatcgcgctc
      961 gccatgtcga ggtcgagcgc ggtctcctgc gcctagccga cgtcgagccg cgggaagctg
     1021 gtgccgccgc gctggtcgac cttgcggaag cagccgttgg cggtggtgca cgccgacagg
     1081 ccgaacgtcg agcggtaggt cgccagatcg ctctcggcgt tggggtaacc gaacgcgtcg
     1141 acgatcgcga tcgtgatcgc cgagctgccg ccgccggtga tcttgtaggc gttgaccagc
     1201 tgggccgggc cgaggcccgc cggggtcgcg ttgggcaccg gcttgccgtc gggaccgacc
     1261 aggacgcggg cgtggcagct cgcctcaccg agctgcgcgg ctgcacacac gcgcgcatgg
     1321 gatgcgctcg cgatctcgtc cgaggtcatc gtcgtcgtga cctcgtcgtc ggcgctcatc
     1381 gcacatgcag gaatggtgag cgcgaacacc aggggtacaa cacgtgatag cgttctgggg
     1441 gtcagttgca tggaacggcc agtggggggc gcgacggtcg gcgtcgcatc acgtgtgctg
     1501 acaaccagcg cgacgccgac cccacagcgc ccgaccccag cgctccacat acggactgct
     1561 ccgtggagtg aatcgctgca gggtcagaac gcggccgcgc cgttcggcgt gccgagcccg
     1621 gtcgggccgt cgtagccggt ccgggccgtg cacaggtagc tcgagctgca gctgccgttg
     1681 ctgcccgacg tgacgtcgaa cagcgagctg gtgtgggcgt acggatccga gccgaaggtc
     1741 acggtgccgc cgttgaccgc gtacaccgcg gcgatcagcg gcgccgcgac gctggtgccg
     1801 ccgaacacca ggaacgccga ggtccccgag ctgctgggtc cgaacaccgc cacgccggtg
     1861 ttgggatcgg cgaccgccga gacatcggcg atggtgcgcc gcgagcaccc ggtgtcgtgc
     1921 tgccagctcg gcctggggta cacggtgctg cagccgctgc ccgcgccgct ccacgcggtc
     1981 tcggtccaac cgcgggtccc atgctcccat tccgcattag acgataggaa atctatatgc
     2041 tgtccatgat ccggattagc aagcacaacg ctgtgtccgg aatgttgcct gtgaagcgcc
     2101 ggtcgcgagc tcgccgcgca cgtgacggct agaacgcgct cgagccgttg ggcgtgccga
     2161 ggccggtcgg gccgtcgtag ccccgggccg cggtgcacgc gtaggtcgga gtgcagaacg
     2221 cgctcgtgct ccccgtggtg acatcgaaca gcgacgcgag gcaggagtag gggttggagc
     2281 cgtagctcac gctaccgcca ttgaccccgt agatcccgcc aatgagcggc gccgccacgc
     2341 tggtgccgcc cagcacgatc cagttcgaga ccccggtcga atccggaccg aacaccgcga
     2401 cgccggtgtt gggatccgcg accgcggcga catcgttcaa catgcgcttc gagcatccgg
     2461 tgtcgtgctg ccagctcggc ttggtataca cggtgctgca cccgctgccg ccaccgtccc
     2521 acgccgtctc ggtccagccg cgggtgttgg ccgcggtcgt gagcgacgtg ccgccgaccg
     2581 cgatcacgtg gggcgacgac gacgggaact ggatgccgcc gaagtacccg gcgtcgcccg
     2641 agctcgcgac gatcgcgact cccgggtggt tgtaggcagg ctcgtaggtc tgggtcccgg
     2701 cctcgtcgcc gccgtagctg ttggtgatca cgtgcgcccc gagcttggcg gcttcgttca
     2761 ccgcggccgc gaggtcggcg aacgtgggcg cggcggcctc gaccaggacg atcttgcact
     2821 tctggcacat cgcgctcacc atctcgaggt cgagcgcggc ctcctggttc cagttgaggt
     2881 tgggcccggg atagctcgtc ccgccgctct gatcgacctt gcggaagcag ccggtgctcg
     2941 acgtgcacgc cggcagtccg aactgcgccc ggtaggtcgc cagatcggcc tccgcggtcg
     3001 cgtagtcgaa tgcagtgatc acggcgatca gcgtcgtgga ggcccccgtg ccggtgatct
     3061 tgtacgcgct gcgcagctgg ctcggtgagt agccggcagg cgcggggttc gtgatcaggt
     3121 ggccgctcgc gtccaccagg ccgaaggcat gacagtaggg cgatccatcc ggggtatccg
     3181 ggcagatcac cgccgagctc gcgctcgtga tctcgtcctg ggtcatcgcg acgttgaaga
     3241 actggtcacc gggcatcgca cacgccgagg caagcgtcgc gacacagagt gggaagactt
     3301 gcaacaaggt ggacgggtgt gagctggtca tgcccgggac gatgcgcgtg cgagtcgggc
     3361 ggcaaaaatc aagtccccta acaattcagg gtggatcttc tgtagcgaac ctcagtatct
     3421 acactcagtt atccggtcgt catccacccg atcgtccacc cgatctcctc agattcctga
     3481 gagatgggtt ggtcgctcga aaacgcatct acactgcacg actccgcacg tatcaaaacg
     3541 atccgggcgt ccgtcctaga acgcattcgc gccgttcggc gtgccgaggc cggtcggacc
     3601 gtcgtagccc ggacccgcgg tgcacgcgta cgtcggatcg cagaacgcgc tcgtgctccc
     3661 ggtggtcacg tcgaacagcg ctccggggtt ggagtagggg ttggagccgt agttcacgct
     3721 accgccattg ttcccgtaga tcccggcgat cagcggcgcc gccacgctgg tgccacccag
     3781 cacgatccac gacgggacgc cggtcgaatc cggcccgaac accgcgacgc cggtgttggg
     3841 atccgcgacc gcggagacgt cgttcagcat gcgcatcgag cagccggtgt cgtgctgcca
     3901 gctcggcttg gggtacaccg cgctgcaacc gctgccgccg ccgtcccacg ccgtctcggc
     3961 ccaatggcgc tggccgttcg ttggggtgag cgatgtgccg ccgaccgcga tgacgtgcgg
     4021 cgacgcggcc gggaactcga taccgccgaa gtacccggag tcgcccgagc tcgcgacgac
     4081 cgcgactccc gggtggttgt aggccgcctc gtagatctcg gtcccgccct cgtagccgcc
     4141 gtagctgttg ctgatcacat gcgccccgag cgcggcggcc tcgttcgtcg cggccgccag
     4201 gtcgccgaac gttgctgcgg tggcctcgac caggacgatc ttgcagcccg ggcacatcgc
     4261 gctcaccatc tcgaggtcaa gcgctgcctc ctggttccag ctaatgttgg gcccggggaa
     4321 gctggtcccg ccgttctgat cgaccttgcg gaagcagccg ctggccgagg tgcacgccgg
     4381 cagcccgaac tgcgatcggt acgtcgccag atcggcctcc gcggtcgcgt agtcgtacgc
     4441 gtcgaccacg gcaatcaacg tcgaggggct ccccgaagtg atcttgtacg cgccgcgcag
     4501 atcgctcggc gaatagcccg ccggcgccga gtgcttgatc aggcggccgt tcgagtcgac
     4561 ctggccgtgg gcgtggcagt agggaccgcc cgaggtcgcc ggagtgcaga gctgcgccac
     4621 gttgacagtg acgacctcgt cctgggtcat cgcgacgtgg aaggacgggt cgtcgggcgt
     4681 cgcacacgcc gaggtaaacg tcgcgacgac gaatggtaag cttcgcaaca cagcagacag
     4741 gtgtgaactg gtcataccat ggacgatgcg cgggtcagct gcgcggaaaa aatcaagccc
     4801 actaacaatt caggattaaa attccgcaat gaacctcagt atttacacac tcaattaacc
     4861 ggtactgcgc cagcctgtcg cctcggattc ctgagggaaa aaacgcctct cgggcacctc
     4921 cctaccgaac ttaggtgtcc tggatcaaaa ggatgcgtgg gagatctgtg tcggcctggc
     4981 gtcattcatc gcacctctcg tcgccgcgat cggccatgaa gg
//
LOCUS       DASVOR010000978         1928 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_506482,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000978 DASVOR010000000
VERSION     DASVOR010000978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506482"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VF516_18945"
     CDS             complement(<1..855)
                     /locus_tag="VF516_18945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109820.1"
                     /translation="MAAANLLIVSDLHFGEELLPGASMERRHAVELGAAAFREFLRYH
                     TVRRRDGRTWRLVIAGDLFDFMSVVIPATRERPAKSADERRFGLGRGIQPGVERMRRI
                     CEAHRPLLTELGRFAAAGHRVDIIVGNHDVELLAREVVGELMRQLAAAGADDRALGRI
                     GVVPWFVHVPGVCWIEHGHVYDEGCSFEFNLAPMDPKDGWLIYNADYAAVRYLGSTVP
                     EIDPHGIEEWGLWGYLRFTMGLGLRSGSRVWLAYARFVRALFAARRLHRSFKRCDRRR
                     REHRARLAE"
     gene            988..>1928
                     /locus_tag="VF516_18950"
     CDS             988..>1928
                     /locus_tag="VF516_18950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX8109821.1"
                     /translation="MTQVLFILLLAASVAFFARTVWMFVRGLAAGTADPRPRLDELPR
                     RLLDVLIYFFGQVKVAEEGPQHRTSKHHLIIFWGFLIITIATADIVVSGVWPGVSLAL
                     LPAVIYQPIYLAVDVMNLLVLLMILWAVFRRLVVRPRLIPWGLDAGLILGAIGSLMVT
                     HFVFHGYEAAAAISAGADAPKFLPISALVGRALAPLSPEAAERGQMMGYWLHVLIVLT
                     FLNYLPYSKHIHLLGALPNILTRNRSERRMDLPKLDLEDENQWGVGRFEQLSWKSLLD
                     TYACTECARCTNYCPAYNTGKNLSPMQLIHDIRYEML"
BASE COUNT          285 a          709 c          641 g          293 t
ORIGIN      
        1 ctcggcgagc cgggcgcggt gctcgcgccg ccggcggtcg cagcgcttga acgagcggtg
       61 cagccggcgc gccgcgaaca gcgcgcgcac gaaccgcgcg taggccagcc acacccggga
      121 ccccgagcgc agccccaggc ccatcgtgaa ccgcaggtag ccccacagcc cccactcctc
      181 gatcccgtgg gggtcgatct cgggcaccgt cgagccgagg tagcggaccg cggcgtagtc
      241 ggcgttgtag atcagccagc cgtccttggg gtccatcggc gcgaggttga actcgaacga
      301 gcagccctcg tcgtagacgt ggccgtgctc gatccagcac accccgggga cgtggacgaa
      361 ccacggcacg accccgatcc ggccgagcgc gcggtcgtcg gcccccgccg cggcgagctg
      421 gcgcatcagc tcgccgacca cctcgcgggc gagcagctcg acgtcgtggt tgccgacgat
      481 gatgtcgacc cggtggccgg cggcggcgaa ccggccgagc tcggtgagca gcggtcggtg
      541 ggcctcgcag atccgccgca tccgctcgac cccgggctgg atgccgcggc ccaggccgaa
      601 ccggcgctcg tcggcggact tcgcgggccg ctcccgggtc gccgggatca ccaccgacat
      661 gaagtcgaac aggtcgccgg cgatcaccag ccgccaggtc cgcccgtcgc gccggcgcac
      721 cgtatggtag cgcaggaact cgcggaacgc cgccgccccg agctcgacgg cgtggcggcg
      781 ctccatcgac gcacccggca ggagctcctc gccgaagtgc aggtcgctca cgatcaggag
      841 attcgcggca gccatgtccc tgcggatcta caagtatgtt ggtttgcgcc tcgccagaca
      901 atcggtgcga gcagctcgca cgcgggcgcg acgaacgccg cgcgctgcca tgacaatcca
      961 gaagctgcgt gatatctccg acacgtcgtg acccaggtcc tgttcattct gttgctcgcg
     1021 gcgtcggtcg cgttcttcgc gcgcacggtg tggatgttcg tgcgcgggct cgccgcgggc
     1081 acggccgatc ccaggccccg gctcgacgag ctgccccggc ggctgctcga cgtcctgatc
     1141 tacttcttcg gccaggtcaa ggtcgccgag gaagggccgc agcaccggac cagcaagcac
     1201 cacctgatca tcttctgggg cttcctgatc atcacgatcg cgaccgccga catcgtcgtg
     1261 tccggcgtgt ggcccggggt gtcgctcgcg ctgctccccg ccgtgatcta ccagccgatc
     1321 tacctcgcgg tcgacgtgat gaacctgctg gtgctgctca tgatcctgtg ggcggtgttc
     1381 cgccggctgg tggtccggcc gcggctgatc ccgtggggcc tcgacgcggg cctgatcctc
     1441 ggcgcgatcg gctcgctgat ggtcacgcac ttcgtgttcc acggctacga ggccgccgcg
     1501 gcgatctcgg ccggcgccga cgcgccgaag ttcctgccga tctcggcgct ggtcggccgc
     1561 gcgctcgcgc cgctgtcgcc cgaggccgcc gagcgcggcc agatgatggg ctactggctg
     1621 cacgtcctga tcgtgctgac gttcctcaac tacctgccct actccaagca catccacctg
     1681 ctcggcgccc tgcccaacat tctgacccgc aaccgctccg agcgcaggat ggatctgccc
     1741 aagctcgacc tcgaggacga gaaccagtgg ggcgtcggcc ggttcgagca gctgtcgtgg
     1801 aagagcctgc tcgacaccta cgcctgcacc gagtgcgcgc gctgcaccaa ctactgcccg
     1861 gcgtacaaca ccggcaagaa cctgtcgccg atgcagctga tccacgacat ccgctacgag
     1921 atgctcga
//
LOCUS       DASVOR010000979         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_360676,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000979 DASVOR010000000
VERSION     DASVOR010000979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360676"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF516_18955"
     CDS             complement(<1..219)
                     /locus_tag="VF516_18955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109822.1"
                     /translation="MRVDGAWRRTIELAPGGDAVIVLDQTALPHRIRWLRLDDLAGAA
                     RAIRDMVVRGAPLIGATAAYGVALALRAG"
     gene            complement(474..1220)
                     /locus_tag="VF516_18960"
     CDS             complement(474..1220)
                     /locus_tag="VF516_18960"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8109823.1"
                     /translation="MAQRKSKRGGKQQPRQRELFRRGGKRRGAGRKSSGARAGERHQA
                     RPDFKPTHALHVVVRVAPAVGSLQCRKLYKAMRDATITAALREWFRIVHISLQRTHVH
                     LLVEAEHKAALSRGMQGFLISAARHINTALGDGARRRRGKVFSDRYHVEVITTPTRAR
                     HAISYILSNWRKHGEDQAELPSTWLVDPFSSAIMFPDWQELDGQPCMWPIREGYDPPM
                     VRRPQTWLLREGWKRAGSAISARAVPSKPR"
BASE COUNT          257 a          599 c          532 g          276 t
ORIGIN      
        1 cccggcgcgc agcgcgagcg ccacgccgta cgcggcggtc gctccgatca gcggcgcgcc
       61 gcgcaccacc atgtcgcgga tcgcgcgtgc ggcgcccgcg aggtcgtcga ggcggagcca
      121 gcggatgcgg tggggcagcg cggtctggtc gagcacgatc accgcgtcgc ctccgggcgc
      181 gagctcgatc gtcctgcgcc atgcgccgtc gaccctcatg gtcgctccgt gcgtaacacg
      241 ccgctggcgc cagggccagg ccggatcatt caccgcggcg tctcggtcga tccgcggctc
      301 atgtggatca acaacccgca cctgaacccg cgcgaggaaa ccgcgtccgg ctccccggct
      361 cgggcgtggt tgcttccgct cgtaacacgc cgccgggccg cgggccagct cacatccacc
      421 ggatcggcca gcaccgaaaa cggaccagct cgcgcgcacc tccgtcgtcg tgttcagcgc
      481 ggcttgctcg gcacggcccg ggcgctgatc gcgcttcctg cgcgcttcca accttcccgg
      541 agcaaccagg tttgcggtct ccgcaccatc ggtggatcgt agccttctcg gatcggccac
      601 atgcatggct gcccgtccag ctcctgccag tcggggaaca tgatcgcgct cgagaacggg
      661 tcgacgagcc aggtgctcgg cagctcggcc tggtcctcgc cgtgcttccg ccagttcgaa
      721 aggatgtagg aaatcgcgtg gcgcgcacga gtcggcgtgg tgatgacctc gacgtgatag
      781 cggtcggaga acaccttgcc gcggcgccgc cgcgcaccgt cgccaagcgc ggtgttgatg
      841 tgcctcgccg ccgagatcag aaagccctgc atcccgcggg acagcgcggc cttgtgctcg
      901 gcctcgacga gcaggtgcac gtgggtgcgc tgcagcgaga tgtgcacgat ccggaaccac
      961 tcgcgcagcg cagcggtgat ggtggcgtcg cgcatcgcct tgtacagctt gcggcactgc
     1021 aggctgccca ccgctggcgc gacccggacc acgacgtgca gcgcgtgggt cggcttgaag
     1081 tccggccgcg cctggtggcg ctctccggcg cgcgctccgc tggactttcg cccggcacca
     1141 cgccgcttgc caccgcgccg gaacagctcc cgctgccgag gctgctgctt gccaccacgc
     1201 ttcgacttcc tctgcgccat gacccatgga tagtcatcgt cgtgcagcct gtcaattgat
     1261 tgggcactac taattgccat tcatttcatc aggcttgatc gccaagcggc attcacccgg
     1321 aatgcaatca gttcgcgaca accacgctcg atcagagaca gatgtctcca gtccttcgca
     1381 accagcgatc gagcttgatg ccactccgac cgatccatgc gatcatgcgc caccgcgttc
     1441 ggctggctgc agaacgcaga ccgctcgctg catccgccgg cccgctcgac ggcgatcgag
     1501 acggcgcgtc gccagcagtc gtcgccgatg gtcctcgacc gcaagctctg cagagcatgt
     1561 ggacggtcct gtggacaact ggaaggtctg atgacacgcg ccacagggcg cagaggtcga
     1621 cctggacgcg tctcacagcc cttcttcagt tgcccacagt cctg
//
LOCUS       DASVOR010000980         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_360681,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000980 DASVOR010000000
VERSION     DASVOR010000980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360681"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VF516_18965"
     CDS             complement(<1..218)
                     /locus_tag="VF516_18965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109824.1"
                     /translation="MIGSWSSGGTRAREHLRQGVEEALVALGQGFVSHADNQALRADL
                     QHGALPVKDYFNQLLRLVYRLLFLLTVE"
     gene            complement(228..1529)
                     /locus_tag="VF516_18970"
     CDS             complement(228..1529)
                     /locus_tag="VF516_18970"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8109825.1"
                     /translation="MKDRVFRDPVHGLIEFKGPDRPIADLLDTAVMQRLRRIKQMGFA
                     WLVYPGAEHSRFGHALGAFHIAQRMTRRLERELSERTALHVKVAALLHDVGHGPFSHA
                     WEHVFAGGGHERWGGRIVAEDPELRALLEALEPDLASTLREFWGKRYKPHFARKLVSS
                     QLDVDRLDYLLRDGHYSGAGYATYDLDWIIHAVQIASVRTGHDDPSDLVVDYRRGMYA
                     VEQYLFARSYMYAQVYHHKTVRAAEWMFIKTLERFAQLTRQGAEPPGLAVAGAMARGS
                     EVAVADYLRLHDVSVTTALDRWAGRDGPGADDPVLHDLAARLVDRRLFKTFDLGDDRP
                     AADRLWPQALEVAERRFGAAARSYLHLDTARQVGYLASDDEELHVIDHPRYGAVTLSR
                     LLEDMPLGQPLAAIRLVCAPELVDDLRPLVEHALGHGRGRR"
     gene            1582..>1865
                     /locus_tag="VF516_18975"
     CDS             1582..>1865
                     /locus_tag="VF516_18975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109826.1"
                     /translation="MSQIRELGIERIDGETRRAERDRVVVEAPLELRARGTPVATIMR
                     TPGHDLELVSGLLHAEAITAGGLAQAGDDAVEVDAAPGAFAGRGLLASAA"
BASE COUNT          261 a          654 c          702 g          248 t
ORIGIN      
        1 tcctcgacgg tgagcaagaa caggagccgg tagacgagcc ggagcagctg gttgaagtag
       61 tccttcaccg gcagcgcgcc atgctggagg tcggcgcgca gggcctggtt gtcggcgtgc
      121 gagacaaagc cctggccgag cgcgacgagc gcttcctcga cgccctgacg caggtgctcg
      181 cgagcgcgcg tgccccccga ggaccatgac ccgatcatcg cggcgaccta ccggcggccg
      241 cggccgtggc ccagcgcgtg ctcgaccagc gggcgcaggt cgtcgaccag ctcgggggcg
      301 cacaccagcc ggatcgccgc cagcggctgg ccgagcggca tgtcctcgag caggcgcgac
      361 agcgtcaccg cgccgtagcg cgggtggtcg atgacgtgga gctcctcgtc gtcggatgcg
      421 aggtagccga cctggcgcgc ggtgtcgagg tggaggtagc tgcgcgccgc ggcgccgaac
      481 cggcgctccg cgacctcgag cgcctggggc catagccggt ccgccgcggg gcggtcatcg
      541 ccgaggtcga aggtcttgaa cagccggcga tcgaccagcc gtgcggcgag gtcgtgcagc
      601 accgggtcgt cggcgcccgg tccgtcgcgg ccggcccagc ggtcgagcgc ggtcgtcacc
      661 gacacgtcgt gcagccgcag gtagtccgcg accgcgacct cgctgccgcg cgccatcgct
      721 ccggcgaccg ccagcccggg cggctcggcg ccctggcggg tcagctgcgc gaaccgctcg
      781 agcgtcttga tgaacatcca ctccgccgcg cgcacggtct tgtggtgata gacctgcgcg
      841 tacatgtacg agcgcgcgaa caggtactgc tcgacggcgt acatcccgcg ccggtagtcg
      901 acgacgaggt cggacggatc gtcgtggccg gtgcgcacgc tggcgatctg cacggcgtgg
      961 atgatccagt cgagatcgta ggtcgcgtag cccgcgcccg agtagtggcc gtcgcgcaag
     1021 aggtagtcga gccggtcgac gtcgagctgc gacgacacca gcttgcgggc gaagtgcggc
     1081 ttgtagcgct tgccccagaa ctcgcgcagc gtgctcgcca ggtcgggctc cagcgcctcg
     1141 agcagcgcgc ggagctcggg gtcctcggcg acgatccggc cgccccagcg ctcgtggccg
     1201 ccgcccgcga acacgtgctc ccaggcgtgc gagaacgggc cgtggccgac gtcgtggagc
     1261 agcgcggcga ccttgacgtg gagcgcggtg cgctccgaca gctcgcgctc gagccgccgc
     1321 gtcatccgct gcgcgatgtg gaacgcgccg agcgcgtggc cgaaccgcga gtgctccgcg
     1381 cccgggtaga ccagccaggc gaaccccatc tgcttgatcc ggcgcagccg ctgcatcacg
     1441 gcggtgtcga gcaggtcggc gatcgggcgg tccggtccct tgaactcgat gagtccatgc
     1501 acgggatcgc ggaacacgcg gtctttcacc cctgcatcgt agcaacaccg cgcggagccc
     1561 gggcagcgct aagatgacgg tgtgagccag atccgcgagc tcgggatcga gcgcatcgat
     1621 ggcgagaccc gccgcgccga gcgcgatcgc gtcgtggtcg aggcgccgct cgagctgcgc
     1681 gcgcgcggca cgccggtcgc gacgatcatg cggacgccgg ggcacgatct ggagctggtc
     1741 tcgggcctgc tccacgccga ggcgatcacc gcgggcgggc tggcgcaggc cggcgatgac
     1801 gccgtcgagg tcgacgccgc cccgggcgcg ttcgccgggc gtggcctgct cgcctcggcg
     1861 gcgtg
//
LOCUS       DASVOR010000981         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_331176,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000981 DASVOR010000000
VERSION     DASVOR010000981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331176"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VF516_18980"
     CDS             complement(<1..1008)
                     /locus_tag="VF516_18980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109827.1"
                     /translation="MTTPAGTLVPRDARDFGSYQLIAKLATGGMAEIFLARPTGSTSR
                     DVLVLKRILPHLAEDEHFVTMFRDEADLASQVSHKNICRVQAFGEYGGTWFIAMEYLH
                     GVPLSRMMTRLSKVGKMLDVRIVAGIIVQACEGLHAAHEARGPDGQPLGLVHRDVSPP
                     NIMVCGDGAVKLLDFGIAKARGANSRTRTGTVKGKNAYMSPEQILGKPLDRRSDVFAL
                     AVVMYEMLAIRRLFHRDSDFLTFKAITEEPIPDIRERRPDLPPGMRAALLQAMARDAN
                     GRFDSAQAFGNALRNAVATLGGPASPADLARLLVTDFADEMIARDEILKAADEPPPPP
                     RP"
     gene            1258..>2483
                     /locus_tag="VF516_18985"
     CDS             1258..>2483
                     /locus_tag="VF516_18985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109828.1"
                     /translation="MREPGVSSSGLEDEVRTAIAEGLVDVELAAQLAEDARRLGRSPL
                     ELLLDRGVITAATLASLRRATFGTGDTLDAPPAMALGPLPSAFALSAGAALFPVPGWD
                     RYEPVRLLGQGAMGRVFLARDLRLRRDVAIKFMRGDEPELARRFVAEARAQARVSHER
                     VCKVYEVGEVEGKVYIAMQHIDGRPLGDLRSELTVEQIAMLVRGAALGVNEAHRAGLI
                     HRDLKPSNIMVERAADGELRSYVMDFGLARDWNEHATMTGTVLGTPHFMAPEQARGEV
                     TRLDRRADVYSLGATLYALLAGKPPFPGDHPLEVLANIAVLEPQPLRAIDRDLPADLE
                     AIPMKCLEKDRSARYDSARALADDLGRFLAGEPVEARATGLGYRLRKRLRKHARLVAG
                     LTVALVVMLGAVGDAVR"
BASE COUNT          345 a          885 c          897 g          356 t
ORIGIN      
        1 cgggcgcggc ggcggcggcg gctcgtcggc ggccttgagg atctcgtcgc gcgcgatcat
       61 ctcgtcggcg aagtcggtga ccaggaggcg cgcgaggtcg gcgggggacg ccggaccgcc
      121 gagcgtcgcc accgcgttgc ggagcgcgtt gccgaacgcc tgcgcgctgt cgaaccggcc
      181 gttggcgtcg cgcgccatcg cctggagcag cgccgcgcgc atcccgggcg gcagatcggg
      241 ccggcgctcg cggatgtcgg ggatcggctc ctcggtgatc gccttgaagg tcaggaagtc
      301 ggagtcgcgg tggaacagcc ggcggatcgc cagcatctcg tacatcacga ccgcgagcgc
      361 gaacacgtcg ctgcgccggt cgagcggctt accgaggatc tgctcgggcg acatgtacgc
      421 gttcttgccc ttgaccgtgc cggtccgggt ccgcgagttg gcgccgcgcg ccttggcgat
      481 gccgaagtcg agcagcttca ccgcgccgtc gccgcacacc atgatgttgg gcggcgacac
      541 gtcgcggtgg accaggccga gcggctggcc atccggaccg cgcgcctcgt gggcggcgtg
      601 cagcccctcg caggcctgga cgatgatgcc ggcgacgatc cggacgtcga gcatcttccc
      661 gaccttggac agccgcgtca tcatccgcga cagcggcacg ccatggaggt actccatcgc
      721 gatgaaccac gtgccgccgt actcgccgaa cgcctgaacc cggcagatgt tcttgtgaga
      781 cacctggctc gcgaggtccg cctcgtcgcg gaacatcgtc acgaagtgct catcctccgc
      841 gaggtgcgga aggatgcgct tgagcaccag gacgtcgcgg ctggtcgatc cggtcgggcg
      901 cgccaggaag atctcggcca tcccacccgt cgcgagcttc gcgatgagct ggtagctgcc
      961 aaaatcccga gcgtcgcgcg ggactagcgt ccccgccgga gtcgtcacgg agccaattat
     1021 gccctagcga ttcccacaat ttaagcccga cctgacaaga gacaccggcg ggcgagggtg
     1081 cgctcgtcgc gccgctggcg ctccgacgag gatgcgagca catcccccga cacccaagcc
     1141 gcggcgcgat ccccggtcgc gacgcgaagc gtgagagccg atacagtacg cctcgagagc
     1201 gagacgagcg cagcgccgtc cggtgcaggc cgcgcgccgc gatatcctcg cccggcgatg
     1261 cgcgagccgg gtgtctcgag cagtgggctg gaggacgagg tccggaccgc gatcgcggag
     1321 gggctcgtcg atgtcgagct ggcggcgcag ctcgccgagg acgctcgccg cctcgggcgc
     1381 tcgccgctcg agctcttgct ggaccggggg gtgatcacgg ccgcgacgct cgcctcgctc
     1441 cggcgggcca ccttcgggac cggtgatacc ctggacgctc cgcccgcgat ggcgctcggc
     1501 cctttgccat cggcgttcgc gctctccgcc ggcgccgcgt tgttcccggt gcctggctgg
     1561 gaccgctacg agccggtgcg cttgctcggc cagggcgcga tgggccgcgt cttcctggcc
     1621 cgcgatctgc ggctgcggcg cgacgtcgcg atcaagttca tgcgtggcga cgagcccgag
     1681 ttggcccgcc gcttcgtcgc cgaggcgcgc gcccaggccc gcgtcagcca cgagcgcgtc
     1741 tgcaaggtct acgaggtcgg cgaggtcgag ggcaaggtct acatcgccat gcagcacatc
     1801 gacgggcgcc cgctcggcga tctgcgcagc gagctcaccg tcgagcagat cgcgatgctg
     1861 gtgcgcggcg ccgcgctcgg cgtgaacgag gcgcaccgcg cgggcctcat ccaccgcgac
     1921 ctcaagccct cgaacatcat ggtcgagcgc gcggccgacg gcgagctgcg gtcctacgtg
     1981 atggactttg gcctggcgcg cgactggaac gagcacgcga cgatgaccgg cacggtgctc
     2041 ggcacgccgc acttcatggc gcccgagcaa gctcgcggtg aggtgacacg cctggatcgc
     2101 cgcgccgacg tctacagcct cggcgcgacg ctgtacgcgc tgctcgccgg caagccgccg
     2161 tttcccggcg accacccgct ggaggtgctc gccaacatcg ccgtcctcga gccgcagccg
     2221 ctgcgggcga tcgaccgcga cctcccggcg gacctcgagg cgatccccat gaagtgcctc
     2281 gagaaggatc gctcggcccg ctatgactcg gcgcgcgcgc tggccgacga cctgggccgg
     2341 ttcctcgccg gcgagccggt cgaggcccgc gcgaccggcc tgggctaccg gctgcgcaag
     2401 cggctgcgca agcacgcgcg gctggtcgcc ggcctgacgg tcgcgctcgt ggtcatgctg
     2461 ggggccgttg gcgacgcggt gcg
//
LOCUS       DASVOR010000982         1657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_126705,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000982 DASVOR010000000
VERSION     DASVOR010000982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126705"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1657)
                     /locus_tag="VF516_18990"
     CDS             complement(<1..>1657)
                     /locus_tag="VF516_18990"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8109829.1"
                     /translation="EAHTGPPNHAGPLTAPVSLQGAALERGTRRAVPGVIVSIAELGL
                     DAVTGDDGTFYFHGVPPGSYKVLAVDPHYDRLERPIALSRQEALEVRLWMRPRGSNPY
                     ETVVEGEREVLEVTRRTIQRQQMTSVPGTFGDPIRVVQTLPGIQRAPFGLGLLLVRGS
                     NPDDTGIYVDGHEVPSLFHFLGGPSIFNAEMLDSLDLYPGGFPARFGRHHGGAVALEL
                     RPSQGGGIHGSAKIDFIDSGGYLRAPITKDLSIAVAGRRSYIDLFLGFVLPQPAKGGQ
                     RIVTPVYYDYSARIDYNLHDNGRLSVFTIGSSDTLHVLNKDPDAALSTDLNTAVKFLR
                     VIGSYERVIAGDLKLTLSPAWGRDTVTFAGAQAEAAGPFSSISVVNDSLSYRMRVHGK
                     LADPLTIDSGLDLLSRVTRYQALLPVDDQLINPQGVDIPPTQLFRGAQLIGLGGYIDL
                     GVDVSSRLKLIPSLRIDGYLLDGQARNSIDPRLVARYQLMPTLTLKAYLGHFSQPPQP
                     EGFDRRFGNPDLGIEHGIHTGIGYEWKPDRLWSIDSELYYVSRY"
BASE COUNT          238 a          516 c          656 g          247 t
ORIGIN      
        1 gtagcggctg acgtagtaca gctcgctgtc gatcgaccac agccggtcgg gcttccactc
       61 gtagccgatg ccggtgtgga tgccgtgctc gatgccgagg tcggggttgc cgaaccggcg
      121 gtcgaagccc tcgggctggg gtggctggct gaagtggccg aggtaggcct tgagggtcag
      181 ggtcggcatc agctggtagc gcgcgaccag ccgcgggtcg atcgagttgc gggcctgccc
      241 gtcgagcagg tagccgtcga tccgcaggct cgggatcagc ttgagccgcg agctgacgtc
      301 gacgccgagg tcgatgtagc cgcccaggcc gatcagctgc gcgccgcgga acagctgggt
      361 cggcgggatg tcgacgccct gcgggttgat cagctggtcg tcgaccggga gcagcgcctg
      421 gtacctggtg acccgcgaca agaggtcgag gccggagtcg atggtgagcg ggtcggcgag
      481 cttgccgtgc acgcgcatgc ggtacgacag cgagtcgttg accacgctga tgctgctgaa
      541 cggcccggcg gcctcggcct gggcgccggc gaacgtcacc gtgtcgcggc cccacgccgg
      601 cgacagggtg agcttgaggt cgccggcgat cacgcgctcg tagctgccga tcacgcggag
      661 gaacttgacc gcggtgttga gatcggtcga cagcgcggcg tcggggtcct tgttgagcac
      721 gtgcagcgtg tcggacgagc cgatcgtgaa cacgctgagc cggccgttgt cgtgcaggtt
      781 gtagtcgatg cgcgccgagt agtcgtagta gaccggggtg acgatgcgct ggccgccctt
      841 ggccggctgg ggcagcacga agccgaggaa caggtcgatg taggaccggc ggccggcgac
      901 cgcgatcgac aggtccttgg tgatcggcgc gcgcaggtag ccgccggagt cgatgaagtc
      961 gatcttggcc gagccgtgga tgccgcctcc ctgcgacggc cggagctcga gcgcgaccgc
     1021 gccgccgtgg tggcggccga accgcgccgg gaacccgccg gggtagaggt cgagcgagtc
     1081 gagcatctcg gcgttgaaga tcgatggacc gccgaggaag tggaacagcg acggcacctc
     1141 gtggccgtcg acgtagatcc cggtgtcgtc ggggttggac ccgcggacca gcagcaggcc
     1201 gaggccgaac ggcgcgcgct ggatgccggg cagggtctgg accacgcgga tcggatcgcc
     1261 gaacgtgccg ggcaccgagg tcatctgctg gcgctggatc gtgcgccggg tgacctcgag
     1321 gacctcgcgc tcgccctcga cgacggtctc gtacgggttg ctgcccctgg gccgcatcca
     1381 cagccggacc tcgagcgcct cctgcctgga cagcgcgatc ggccgctcga ggcggtcgta
     1441 gtgcggatcg accgcgagca ccttgtagct gccgggcggc acgccgtgga agtagaacgt
     1501 gccgtcgtcg ccggtgaccg cgtcgaggcc gagctcggcg atcgagacga tgacgccggg
     1561 gacggcgcgc cgcgtgccgc gctcgagggc ggcgccctgc agcgacaccg gcgcggtgag
     1621 cgggccggcg tggttgggtg ggccggtgtg ggcctcc
//
LOCUS       DASVOR010000983         3516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_506525,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000983 DASVOR010000000
VERSION     DASVOR010000983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3516
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506525"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1551
                     /locus_tag="VF516_18995"
     CDS             466..1551
                     /locus_tag="VF516_18995"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX8109830.1"
                     /translation="MYKSLTAQTLHYFARPHEVPRRTPIDSPAAWRGGELATSVAWRH
                     PLSSGDVDELDRALAAAKASGRPQRALRAADFPLPRLTESIVAWRREIADGRGFVLVQ
                     GLPVERWGPDDSAIVFYGIGLHLGIPGAQNPEGDLLGHVRDTGADPRAARAYRTASNI
                     AYHCDAADLVGLLCLETARHGGLSRIVSSITVYNTLLAEQPELVDRLYQPFLLDTHGE
                     GGVDCIPIPACRYAGGRLRTFWHSDYFRSSHDYRRAPAFDPRERAVLDAYDAIASRPE
                     LYLDMELAKGDAQLLSNHTILHARTAYEDHSEPARKRHLLRLWVSLDTQPRFRDRLAR
                     LRGGVPLLAALARARIDRAVRGRHERR"
     gene            1747..2298
                     /locus_tag="VF516_19000"
     CDS             1747..2298
                     /locus_tag="VF516_19000"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8109831.1"
                     /translation="MAEMDPPTPEMLAALVASHREFLSYVERRVGDRALAEDILQDAL
                     VRSLDKVGDVQESLIGWFYRVLRNAIIDHVRRRAAQDRRLAAMEGGSPTHADAALEDV
                     VCRCVTKLAETLKPEYAVALRRVDVEGLSVKDYANEAGISPNNASVRMFRAREALRKQ
                     VARACGTCAEHGCFDCTCKAAPS"
     gene            2735..3120
                     /locus_tag="VF516_19005"
                     /pseudo
     CDS             2735..3120
                     /locus_tag="VF516_19005"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
     gene            3179..>3516
                     /locus_tag="VF516_19010"
     CDS             3179..>3516
                     /locus_tag="VF516_19010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-transporting ATPase"
                     /protein_id="HEX8109832.1"
                     /translation="MKRRFFVAVLLTVPVLVLAMSEMFASAWLSGRTRNYVELALATP
                     VCLWSAWPFFVRAAQSVVHRSLNMFTLIGLGVGVAYGYSVIATLFPEVFPVSFRDHHG
                     QVGVYFEAASV"
BASE COUNT          520 a         1228 c         1225 g          543 t
ORIGIN      
        1 cgcgaaccgg gcgtagatcc ccggctcgcc ggtcttcttg tgctcgagga gctggttggt
       61 cacgccggcg tacgccgaga cgacgtacac gcggccgtac acgtggtggg ggtcgcgcag
      121 catgatgttg cgcagcacgt cctcgaagcg cgacatcgag gtaccgccga ttttttcgac
      181 cgtgatcatg gtggcgtccg cgtgcaggga cgaggagaac taagcacgaa accggtgcgc
      241 gggtcacgca cgcagcgcgc cgcgtgagat cccggctcac gccgaggcgg cggtcagcgg
      301 ggctcgctcg cgggtctcga gggcgccggc gacgtcgacc accgcgcgcg agggcccggc
      361 gcgcgatcgg ccgcagtgcg cggccggcca cgcaggcatg cggacgcggc gaccgggaat
      421 acctggacgc ggcgggtcga cagatgtcat cgttgcagag atcccgtgta caaatcgctc
      481 actgcgcaga cgctccacta cttcgcgcgc ccgcacgagg tgcctcggcg aaccccgatc
      541 gacagccccg cggcgtggcg cggtggcgag ctcgcgacga gcgtcgcgtg gcgtcacccg
      601 ctgtcgagtg gcgacgtcga cgaactggat cgcgcgctcg cagccgcgaa agcgtcgggc
      661 cgcccgcagc gcgcgctgcg cgcggccgac ttcccgctgc cgaggctgac cgaaagcatc
      721 gtcgcgtggc gccgcgagat cgccgatggc cgcggcttcg tcctcgtgca gggccttccg
      781 gtcgagcgct gggggccgga cgactcggcg atcgtgttct acggcatcgg tcttcacctc
      841 ggcatccccg gcgcgcagaa cccggaaggt gatctgctcg gtcacgtccg cgataccggc
      901 gccgaccctc gtgcggcccg cgcataccgg accgcgtcga acatcgccta ccactgcgac
      961 gcggcggatc tcgtcggcct gctctgcctg gagaccgcgc gccacggcgg gctgagcagg
     1021 atcgtcagct cgatcacggt ctacaacacg ctgctggccg agcagcccga gctcgtcgac
     1081 cgactgtacc agccgtttct gctcgacacc catggcgagg gcggcgtcga ctgcattccg
     1141 atcccggcgt gccgctacgc gggtggccgg ctgcgcacgt tctggcactc cgactatttt
     1201 cgctcgtcgc acgactaccg gcgcgcgccc gcgttcgatc cgcgcgagcg cgccgtgctc
     1261 gatgcatacg acgcgatcgc gtcgcggccc gagctgtacc tcgacatgga gctcgcgaag
     1321 ggcgacgccc agctgctatc gaaccacacg attctccacg cccgcaccgc gtatgaggat
     1381 cattcggagc cggcgcgcaa gcgccacctg ctgcggctgt gggtctcgct cgacacgcag
     1441 ccccggtttc gcgatcgcct cgctcgcctc cggggcggtg tgccgctgct cgccgcgctc
     1501 gctcgcgcgc ggatcgatcg agccgtgcgg ggccgacacg agcgccgctg agcggtggca
     1561 tgcgcgcgaa tggggtcccg tgctggctac cctgcgcatc cgagcgcaaa ccgcggccgg
     1621 cgctggactt cgcagccggc tcgaccggcg aagccgtcac tcctcggtca gcagccgatc
     1681 ggcggcgggt tgcaggctcg gccgtatgtc gcgatgccgg agccagcggg atactacagt
     1741 gcctgcatgg ccgagatgga tcccccgact cccgagatgc tcgcggcgct cgtcgcgagc
     1801 catcgcgagt ttctttcgta cgtggagcgt cgcgtcggtg accgcgcgct cgccgaggac
     1861 atcctgcagg acgcccttgt ccgcagtctc gacaaggtgg gcgacgtcca ggaatcgctg
     1921 atcgggtggt tctatcgcgt gcttcgcaac gcgatcatcg atcacgtccg gcggcgcgcg
     1981 gcgcaggatc ggcggctcgc agccatggaa ggtggatccc ccacgcacgc cgacgcggcc
     2041 ctcgaggacg tggtgtgccg gtgcgtgacc aagctcgccg agaccctcaa gcccgagtac
     2101 gccgttgcgt tgcggcgggt cgacgtcgaa ggactgtccg tgaaggatta cgcgaacgag
     2161 gccggcatct cgccgaacaa cgccagcgtg cgcatgttcc gggcgcgcga ggcactgcgc
     2221 aagcaggtgg cgcgcgcgtg cgggacctgc gccgagcacg gctgcttcga ctgcacgtgc
     2281 aaagctgctc cgtcgtgatt tcgcggggat gacgcgctcg aagatccagc tgatcgcacg
     2341 ggtgtaatgc cgcggggccc ggtgcgtcgt tcggcaggag cgacccacca tgaaccccac
     2401 cgagcatcat cattgccacg gtctcgcggc tgcgccagta gctggcgccg agcgcgcctt
     2461 ggatccggtc tgcgggacga ccgcgtccgg tggcgcccgc ggagacagcg cagtccacgc
     2521 cagcggcgcg tgctgtcacc catgttgccg cgacacgctc agcgccgatc cggcggcaca
     2581 tcccgcgccg gaggcgaagg cgccgcgatc gccgagcgcg cgacagccat ctggatcttc
     2641 gaacgctggt aacccgggtc cttgccttgc aagaccgacc ggcatgcatc gcacagagca
     2701 ccatcacggc cacgagccag ccactgcgcc cccgatgacg ccggcgaggg acccggtttg
     2761 cgggatgacc gtggacctgg cgacggcaaa gggcggccag gtcgagcatg aggggcgcga
     2821 ctacgcgttc tgtggcctgt cgtgccgcaa caagtttctc gcatatcctg cgcgctacgc
     2881 ggcgcccgcg cgggccttgc acgaagcacc ggccgcagcg cgggctccgg agaccggcac
     2941 cgtgtacacg tgtccgatgc accccgaggt gcggtcatct acgcccggga gctgccccaa
     3001 gtgcggcatg gcgctggagc caatcctccc ggtacgcacc gcgcccacaa cgcagtggac
     3061 ctgcccgatg cacccggaga tcgtgcgcga tgcgccaggc gcctgcccga tctgcggcat
     3121 ggcgctggag tcgcgtaccg tcacagcgga gccggtggag gaccacgagc tacgcgacat
     3181 gaagcgacgc tttttcgtcg cggtgctgct cacggttccg gtgctggtcc tcgcgatgtc
     3241 ggagatgttc gcgtccgcct ggctgtcggg aaggacgcgc aactacgtcg agctcgcgct
     3301 ggccacgccg gtgtgcctgt ggtcggcgtg gccgttcttc gtacgcgcgg cgcagtccgt
     3361 cgttcacaga agcctgaaca tgttcacgct gatcggcctc ggcgtgggcg tggcgtacgg
     3421 ctacagcgtc atcgcgacgc tgttccccga ggtgtttccg gtgagcttcc gggatcacca
     3481 cggccaggtc ggcgtgtact tcgaggcagc ctcggt
//
LOCUS       DASVOR010000984         3802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_331187,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000984 DASVOR010000000
VERSION     DASVOR010000984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331187"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF516_19015"
     CDS             complement(<1..251)
                     /locus_tag="VF516_19015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109833.1"
                     /translation="MIARIELTKRGYGRSRGKHGRIRLPRPAATGARGTATLRMVERS
                     RLSRFGLVLLTLITLGWGFNWSVVKLVLVEIPPLTLRAI"
     gene            complement(480..1382)
                     /locus_tag="VF516_19020"
     CDS             complement(480..1382)
                     /locus_tag="VF516_19020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109834.1"
                     /translation="MLGADDDDALYVFEREPRDLRLPPTRRRGEDAGQSLKILKRRLG
                     IADVSLTADLEKLLSRYCSDTILNILWYDRARRREQRANARRTYLMVLLMIIALVLLC
                     ASAFLPSERSASGAAMLPFALLAGGSLTVLQVVAALSDGKARLSIFWKAGADLKEALY
                     NFEHRWRGRVFHGDPPCVAAEFDAAVQEELKFAYAVTRAERLEFFANLRSPSDVFAVA
                     MGAMNNLRERRVESSAALATRDDQIAAARKSVSEARAAVLASEYRLQAAADGSSKVAE
                     QTALVNARAEVVRTEAILHEIVGG"
     gene            1710..>3802
                     /locus_tag="VF516_19025"
     CDS             1710..>3802
                     /locus_tag="VF516_19025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109835.1"
                     /translation="MDAGAEGIHLVWSWPDTLPLSEGGFDIQRLGSTDQRWESLCEDI
                     GASQIAYLRQWHTYPAPLGPLRLRTGARFAPIGDPRLAPKSSAAAHAIDIGDPHRDLS
                     AALRTAIASSTVPAAAVVNQPIDEFIQELDAPAERVSVEVHARAAIAMALCAGKVVQV
                     APAATLPATLQLMAPAIDTVVVYTVAPTALRICAYRRVDDAKGGPWASAPYIVKGLTL
                     PIHETDPSLATPAQEYAAAHARVVAGENLTAADFARLTVSLRGPAGSPGLGRSGERIV
                     LVRAATDQSFEELPFDTQLAALAIHPKARRVLGFGFRDTKGLVAGATYSYRITGRFRA
                     EDLTDAIYDVHRVPASTVLPAAFSIRDLGLRFQTPVKVVLDPAPPATATHAVSRRGIR
                     VDTSGYDASWVLPFYGSWSALITFPRLVSKVVLEVATGHSFQYAAGLPWAFAGAPTPL
                     PPGPRVELAFGSPVQELRLSGKGTLYAVRLPSGETGIREVHAYTQPITYAASPLPVPP
                     IALTIENLQSPPSTITGPIDDATPVPPRAPVGFKLHWLPATTAGIAMWPDDLEAGPPL
                     DALAYQIEHRRDHPSPTPFAPIAGDDNLTLGSRDGIAPSVRLEYGCDLDALFPHTRPR
                     SPGAGLVLSLSDVFGQKDPTGFDRPAEPLGTYHQYQIRAVDAVGRVSATATLSNLERL
                     EKHVPPPIPVGPQPEP"
BASE COUNT          574 a         1373 c         1306 g          549 t
ORIGIN      
        1 cagatggcgc gaagcgtgag cggcgggatc tcgacgagga cgagcttgac gacggaccag
       61 ttgaagcccc agccgagggt gatcagcgtg agcaggacga ggccgaaccg ggacagtctg
      121 gagcgttcca ccatgcggag agtagccgtg ccgcgagcgc cggtcgctgc cggacgtggc
      181 aagcggatgc gcccgtgctt gccgcgcgag cggccgtagc cgcgcttggt gagctcgatc
      241 cgtgcgatca cgcccttgtg gacctcgtca ggggccctcg gcgagccgca gcgtgcgggc
      301 ctgggcgaag aagctggtca acgggcgctg cgcgccgccg ccacccggtc tacaatcgcc
      361 ccggcactgg ttgccatccg cgggccgggg tcacagaagc accgcgtgga tcgtccaccg
      421 atcggcccgc aagcggtggg tcggtaccga gggcgcgggg ctgtacgtgc ggccgaccct
      481 cacccaccga cgatctcgtg caagatcgcc tcggtgcgga cgacctcggc gcgcgcattg
      541 accagcgcgg tctgttccgc gaccttgctg gaaccgtcgg cggcggcctg caagcggtat
      601 tcggatgcga gcaccgccgc gcgggcctca gacacgctct ttcgcgcggc ggcgatctgg
      661 tcgtcgcgcg tcgccaaggc ggcgctgctt tcgacgcgcc gctcgcgcag attgttcatc
      721 gcgcccatcg ccactgcaaa gacatccgat ggcgaacgca gattcgcaaa gaactccaga
      781 cgctccgctc gcgtcactgc gtatgcgaac ttcagctctt cctgcaccgc agcgtcgaac
      841 tccgcagcca cgcaaggtgg gtcaccgtga aacacccgac cgcgccatct gtgctcgaag
      901 ttatagagcg cttccttgag atcggcaccc gccttccaga agatcgagag cctcgccttg
      961 ccgtcggaca gcgcggcgac gacctggagc acggtgagcg agccacctgc caagagcgcg
     1021 aacggcagca tcgccgcccc cgaggcgctg cgctcggagg gcaagaatgc cgacgcacac
     1081 aacaggacca aggcgatgat catgagcagg accatgaggt acgtcctgcg cgcgtttgcc
     1141 cgttgctccc gccttcgagc tcggtcgtac cagaggatgt tgaggatcgt atccgagcaa
     1201 tatcgcgaca gcagcttctc caggtccgcc gtgagcgaca cgtcggcgat accgaggcga
     1261 cgtttcaaga tcttgagtga ctgccctgca tcctccccgc ggcgccgggt cggcggcagg
     1321 cggagatccc tgggttcccg ctcgaacaca tagagcgcat cgtcgtcatc agccccgagc
     1381 attgtcgatg gacacgacga tacttgccca ggcgtagggc cgcaagcgcg cacgaggcgg
     1441 ccgagctagc cttcccgctc cgacgaccag cgttcgtagt gggctccgct accctttccg
     1501 ctcaccgcgt gagccggtag cgcccggtcc atcaggataa ggagcgtcca ggccaggcca
     1561 agcttgccag cgtctacatg cactcccgca gcgatgcgtt ttctcgtgcg ttgtgctcgc
     1621 acgcaattgc gcgcgcgcgc ggcgcaccga tatagtggca acacttcttc gcggggggat
     1681 gacacgtgca ggtcttcggc attccccgct tggatgcggg ggcagagggc attcatctgg
     1741 tctggtcgtg gccagacacc ctgccgctct ccgagggcgg cttcgacatc cagcggctcg
     1801 gcagcaccga ccagcgatgg gagtcgctgt gcgaggacat cggcgcgtcg cagatcgcgt
     1861 acctccggca gtggcatacg tacccagcgc cgctcgggcc gctgcgattg cgcaccggcg
     1921 cacggtttgc accgatcggg gatcccaggc tggcgccgaa gagctccgcc gcggcgcacg
     1981 cgatcgacat cggcgatccg caccgcgacc tgagcgccgc gctgcgcacc gcgatcgcca
     2041 gctcgaccgt ccccgccgcg gcggtcgtca accagccgat cgacgagttc atccaggagc
     2101 tcgacgcgcc ggccgagcgg gtgtcggtcg aggtccacgc ccgcgcggcg atcgcgatgg
     2161 cgctgtgcgc cggcaaggtc gtgcaggtcg cgcccgccgc gacgctgccg gcgacgctgc
     2221 agctgatggc gcccgcgatc gacaccgtcg tggtgtacac ggttgcgccg accgcgctgc
     2281 ggatctgcgc gtaccggcgc gtcgacgatg cgaagggcgg cccgtgggcg agcgcgccat
     2341 acatcgtcaa ggggctgacg ctgccgatcc acgagaccga cccgagcctg gcgacgccgg
     2401 cgcaggagta cgccgcggcg cacgcacgcg tggtcgccgg ggagaacctc acggcggccg
     2461 acttcgcgcg gctcacggtg tcgctgcgcg gaccggcggg gagcccgggc ctgggccgca
     2521 gcggcgagcg catcgtgctg gtccgcgccg cgaccgacca gagcttcgag gagctgccgt
     2581 tcgacaccca gctcgcggcg ctcgcgatcc accccaaggc gcggcgcgtg ctcggcttcg
     2641 gattccgtga caccaagggg ctggtcgcgg gcgcgaccta cagctaccgg atcaccgggc
     2701 ggttccgcgc ggaggacctg accgacgcga tctacgacgt gcaccgcgtg ccggcgtcga
     2761 cggtgctgcc cgcagcattc tcgatccgcg acctcggcct gcggttccag acgccggtga
     2821 aggtcgtgct cgaccccgcg ccgccggcga ccgcgacgca cgcggtgagc cggcgaggga
     2881 tccgggtcga caccagcggc tacgacgcgt cgtgggtgct gccgttctac gggtcgtgga
     2941 gcgcgctgat cacgttcccg cgcctggtct ccaaggtcgt gctcgaggtc gcgaccgggc
     3001 attcgttcca gtacgcggcc gggctgccgt gggcgttcgc cggcgcgccg accccgctgc
     3061 cacccgggcc gcgcgtcgag ctggcgttcg gctcgccggt gcaggagctg cggctgtcgg
     3121 gcaagggtac gctgtacgcg gtgcggctcc cgtcgggcga gaccggaatc cgcgaggtcc
     3181 acgcctacac ccagccgatc acctacgcgg catcgccgct gcccgtgccg ccgatcgcgc
     3241 tcacgatcga gaacctccag tcgccgccgt cgacgatcac cggcccgatc gacgacgcca
     3301 cgccggtccc gccgcgcgcg ccggtcggct tcaagctgca ctggctgccg gcgaccacgg
     3361 ccggcatcgc gatgtggccc gacgatctcg aggccggtcc gccgctcgac gccctcgcct
     3421 accagatcga gcaccgccgc gatcatccgt cgccgacgcc gttcgcgccg atcgccggcg
     3481 acgacaacct cacgctcggc tcgcgcgacg gcatcgcgcc gtcggtgcgg ctcgagtacg
     3541 gctgcgatct cgacgcgctg ttcccgcaca cccggccgcg ctcgccgggc gccggcctcg
     3601 tgctgtcgct gagcgacgtg ttcggccaga aggatccgac cgggttcgac cggcccgccg
     3661 agccgctcgg cacgtatcac cagtaccaga tccgcgcggt cgacgcggtc ggccgggtca
     3721 gcgccacggc gacgctgtcc aacctcgagc ggctcgagaa gcacgtgccg ccgccgatcc
     3781 cggtcggccc gcaacccgag cc
//
LOCUS       DASVOR010000985         4193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448082,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000985 DASVOR010000000
VERSION     DASVOR010000985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4193
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448082"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2225
                     /gene="gspD"
                     /locus_tag="VF516_19030"
     CDS             <1..2225
                     /gene="gspD"
                     /locus_tag="VF516_19030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HEX8109836.1"
                     /translation="GAGPAAAEPVAGHPPPGESPAGDEPPFRCKPHPGPVVVTFKADA
                     EVKDLIAWVMGFTCKNVLLDPRVTATGRRISIVTPSPMSPDDAYQVFLAALATINLTV
                     VTRGNTLRIVEANGAHKEAVPLLHAGTPGDTDQYVRYVYKPSFVTAEQLHQAYQAMKS
                     DAGDVFTLGQVVVITDYASHVREMLAFARLVDVPGGSDGIYTLPVHHADAGKLAEKLN
                     AILNLPPGAAPIRPAPDPGKPDPGRSATAAVPSKIVVDERTNTLIVASSEAGYQRVQA
                     LAERLDVALEMESGSAIHVVPLGGAIAEELAKTLTQAIGDGHASRSTVPGGPPSAPPS
                     APPRSGPGIAPPAPTAPPAAPATPLDPAVDGLGATIEGQVRVIADPQTNALIVMANAR
                     DFVAIKDVIRQLDQPRRQISLEVVILEVEAGTDHTLGSASHGGQTLGGDTGQVLFGGV
                     RTSDVNTLDVLHSLQSVTGGFAGLTGPTISLLGQSIPSYTVLFQALAQQTHSNIISSQ
                     TITAVDNVLAKFKVGSKRPVNMGNTITPFGSTTVSTPRIEFPEFPLKLDIKPHISSDD
                     SVLLEVSQDAEELVNLTALGPITNTRMFDTRVVVHDQETVVLSGLSQERESTDTTKVP
                     LLGDIPLLGYLFKTTTTSKLRTNLLVLLTPYIVKDRRDLQAFRERKLREYDEFARSMS
                     GLSHMAFQPDVDYRKKRGLIEEINRAVLDAEHDAAARAAVDAHPRGVTAGSVEPPREP
                     "
     gene            complement(2247..2993)
                     /locus_tag="VF516_19035"
     CDS             complement(2247..2993)
                     /locus_tag="VF516_19035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109837.1"
                     /translation="MTIRTVLGAAGVLVIGGMLGCAANSDYVYQPDTANATAAGLPAA
                     RTPIPQEAPQGAIEVTSYGVTQLRRDGAAIPALHVRAIVTNDGDAQPWTLDTTQQLVE
                     IPGEGRSRPIFVNAEVGTLPNVAIALHERRVIDLYYALPDTIRSASQLPRFDLLWQVN
                     TPARAVASRTSFDRVDREPQVASTYDTAPWPVWAGFGPYWWYDPFYPSVVFVHARPYH
                     LHGNGRVVVGGFGGRFHAGSGAHVAGGIHH"
     gene            complement(3397..3555)
                     /locus_tag="VF516_19040"
     CDS             complement(3397..3555)
                     /locus_tag="VF516_19040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109838.1"
                     /translation="MLSGTAGEVGVGVSFDWSSGGVGWLAPGSLDVPDDASDGDAGDG
                     GVEGGGWA"
     gene            complement(3556..3714)
                     /locus_tag="VF516_19045"
     CDS             complement(3556..3714)
                     /locus_tag="VF516_19045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109839.1"
                     /translation="MKANISVFVSVPGSSGPGAGTGAGAVVPPTEVVVVVVVVVDDVV
                     SGGLGGGG"
     gene            3701..>4193
                     /locus_tag="VF516_19050"
     CDS             3701..>4193
                     /locus_tag="VF516_19050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109840.1"
                     /translation="MFAFNWHNPGLRSGMGVSVLAGGGVVQFTKSTMRSTTSDLGGLW
                     NVHATFGTHLPLALDLAYVGSATHIGGLPTGGTATLIGTAAEGTLRWNVLPNFAWTPY
                     IFGGVGYQRYDVKGGDVSLSDSGMNSSDNLLSVPAGLGFVWRGNNGLVADVHGTYRFT
                     WFQD"
BASE COUNT          668 a         1571 c         1400 g          554 t
ORIGIN      
        1 cgggggccgg ccccgccgcg gccgagccgg tggccggtca cccgccgccc ggcgagtcgc
       61 ccgccggcga cgagccgccg ttccgctgca agccccaccc gggaccggtg gtcgtgacgt
      121 tcaaggccga cgccgaggtc aaggacctga tcgcgtgggt gatgggcttc acctgcaaga
      181 acgtcctgct cgacccgcgg gtcaccgcga ccggccgcag gatctcgatc gtcacgccca
      241 gcccgatgag ccccgacgac gcctaccagg tgttcctcgc cgcgctggcg acgatcaacc
      301 tcaccgtcgt gacccgcggc aacacgctgc ggatcgtcga ggccaacggt gcccacaagg
      361 aggccgtgcc gctgcttcac gccgggaccc cgggcgacac cgaccagtac gtgcgctacg
      421 tctacaagcc gagcttcgtc acggccgagc agctccacca ggcgtaccag gcgatgaagt
      481 ccgatgccgg cgacgtgttc acgctcggcc aggtcgtggt gatcaccgac tacgccagcc
      541 acgtgcgcga gatgctcgcg ttcgcccggc tggtcgacgt gcccggcggc agcgacggga
      601 tctacaccct gcccgtccac cacgccgacg ccggcaagct cgccgagaag ctgaacgcga
      661 tcctcaacct gccccccggc gcggccccga tccgaccggc gcccgatccc ggcaagcccg
      721 atcccggcag gtcggcgacc gcggccgtgc cgtcgaagat cgtcgtcgac gagcgcacca
      781 acacgctgat cgtcgcgtcg agcgaggccg gctaccagcg ggtccaggcg ctggcagagc
      841 ggctcgacgt cgcgctcgag atggagagcg gcagcgcgat ccacgtggtc ccgctcggcg
      901 gcgcgatcgc cgaggagctc gcgaagacgc tgacccaggc gatcggcgat ggccacgctt
      961 cccgttcgac ggtccccggt gggcctccct cggcgccgcc gtccgcgccg ccgcgatccg
     1021 ggcccggcat cgcgccgccc gcgcccaccg ccccgccagc ggcgccggcc acgccgctgg
     1081 atccggcggt cgacggcctc ggcgcgacga tcgagggcca ggtccgcgtg atcgccgatc
     1141 cgcagaccaa cgcgctgatc gtgatggcga acgcgcgcga cttcgtcgcg atcaaggacg
     1201 tgatccggca gctcgatcag ccgcgccggc agatctcgct cgaggtcgtg atcctggaag
     1261 tcgaggccgg caccgaccac acgctcggca gcgcgtcgca cggcggtcag acgctcggcg
     1321 gtgacaccgg ccaggtcctg ttcggcgggg tcaggacctc ggatgtcaac acgctcgacg
     1381 tcctgcactc gctgcagagc gtgaccggcg ggttcgccgg cctcaccggc cccacgatct
     1441 cgctgctcgg ccagagtatc ccgtcgtaca ccgtgctgtt ccaggcgctg gcccagcaga
     1501 cccacagcaa catcatctcg tcgcagacca tcaccgccgt cgacaacgtg ctcgcgaagt
     1561 tcaaggtcgg cagcaagcgc ccggtcaaca tgggcaacac gatcacgccg ttcggcagca
     1621 ccaccgtgtc cacgccccgg atcgagttcc ccgagttccc gctcaagctc gacatcaagc
     1681 cccacatctc gagcgacgac tccgtgctgc tcgaggtctc gcaggacgcc gaggagctgg
     1741 tcaacctgac cgcgctgggc ccgatcacca acacccggat gttcgacacc cgcgtggtcg
     1801 tccacgacca ggagaccgtg gtgctgagcg gcctgagcca ggagcgcgag agcacggaca
     1861 ccaccaaggt gccgctgctc ggtgacatcc cgctgctcgg ctacctgttc aagaccacga
     1921 ccacgtcgaa gctccggacc aacctgctcg tcctgctgac cccgtacatc gtcaaggacc
     1981 ggcgcgacct ccaggcgttc cgcgagcgca agctgcgcga gtacgacgag ttcgcgcgct
     2041 cgatgtccgg gctatcgcac atggcgttcc agccggacgt cgactaccgc aagaagcgcg
     2101 gcctgatcga ggagatcaac cgcgcggtgc tcgacgccga gcacgacgcc gccgcgcgcg
     2161 ccgcggtcga cgcccacccg cgcggcgtga ccgccggcag cgtcgaaccg ccgcgggaac
     2221 cgtgaccgcc gctcggtgcc cgcggttcag tgatgaatgc cgccggcgac gtgcgcgccg
     2281 ctgccggcgt ggaaccgacc gccgaacccg ccgaccacga cccggccgtt gccatggagg
     2341 tggtacgggc gggcgtggac gaacacgacg ctgggataga acgggtcgta ccaccagtac
     2401 gggccgaagc cggcccacac gggccacggc gcggtgtcgt acgtcgacgc gacctggggc
     2461 tcgcgatcga cgcggtcgaa cgaggtccgc gacgcgaccg cccgcgccgg cgtgttgacc
     2521 tgccacagca gatcgaaccg cggcagctgg ctcgccgacc ggatcgtgtc gggcagcgcg
     2581 tagtacaggt cgatcacccg gcgctcgtgg agcgcgatcg ccacgttggg cagggtgccg
     2641 acctcggcgt tgacgaagat cggccggctg cggccctcgc cggggatctc gaccagctgc
     2701 tgggtcgtgt cgagcgtcca cggctgggca tcgccgtcgt tggtcacgat cgcacggacg
     2761 tgcagcgccg ggatcgccgc cccatcgcgc cgcagctggg tcaccccgta cgacgtcacc
     2821 tcgatcgcgc cctgcggtgc ctcctggggg atcggggtcc gcgcggcggg cagcccggcc
     2881 gccgtcgcgt tggcggtgtc gggttgatag acgtaatccg aattcgccgc acaaccgagc
     2941 atccccccga tcaccaggac tccggcggcg ccgagtacag tgcgaatcgt catggcacta
     3001 ctcctttgca tgatgccgtt gcaagaatcg taatcggcgt cccgacgacg ccgtgtctcg
     3061 gcgcgcgtcg gaatcccccg agttcggcat cgcccgtgcc agcgcggcct gcgacgtacc
     3121 agcacgatga ccgtgcggcg tgcgggccag ccccgcagcg cggcgtcggg aatgttcgca
     3181 aaccgtgcgc gggcggccta ccgggacgag ccaccgacgg cctgcgccgg cctgcggtgg
     3241 acgccgaccg ggggccgcct agtggcatgc acgatgctcg tcgaggtggg tgagttagca
     3301 aggagagcct tcgatgcgag agatgacaaa gatcctgctg tccgcgtgta cggtgtgcgc
     3361 cctcgggcaa ggcaacatcg cgcgagcgca gaacggtcag gcccagccac cgccctcgac
     3421 cccgccgtcg ccggcgtcgc cgtcggacgc atcgtccgga acgtcgagcg agccgggcgc
     3481 cagccagccg acgcctccgg acgaccagtc gaacgagacc ccgaccccga cctcgccggc
     3541 cgttccggat agcaatcagc cgcctccgcc cagcccgccg gatacgacat cgtcgacgac
     3601 gaccaccacc acgacgacca cctcggtcgg cggcacgacc gcgccggccc ccgtcccggc
     3661 cccgggcccc gaggagcctg gcaccgagac gaacaccgag atgttcgcct tcaactggca
     3721 caatcccggc ctgcggtcgg gcatgggggt cagcgtgctc gccggcggcg gcgtcgtcca
     3781 gttcaccaaa tcgacgatgc gcagcacgac gtcggacctc ggcggcctgt ggaatgtcca
     3841 cgcgacgttt ggcacccacc tcccgctggc gctcgacctc gcctacgtcg ggtcggcgac
     3901 ccacatcggc ggcctgccga ccggcggcac cgccacgctg atcggcaccg cggccgaggg
     3961 tacgctgcgc tggaacgtgc taccgaactt cgcctggacg ccgtacatct tcggcggcgt
     4021 ggggtaccag cgctacgacg tgaaaggcgg cgacgtgtcg ctgtccgaca gcggcatgaa
     4081 ctcgagcgac aacctgctga gcgtcccggc cggcctcggg ttcgtgtggc gcggcaacaa
     4141 cgggctggtc gccgacgtcc acggcaccta ccgcttcacc tggttccagg atc
//
LOCUS       DASVOR010000986         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448087,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000986 DASVOR010000000
VERSION     DASVOR010000986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448087"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VF516_19055"
     CDS             complement(<1..998)
                     /locus_tag="VF516_19055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109841.1"
                     /translation="MEQFGKYSLIRKIGTGGMAEVFLARTTVAQGLNKTLVIKKIHSA
                     YARSRQFVAMFVDEAKIALGLNHPNVTQVFDFGAVADTYFLAMEYVEGVDLLRLLQEA
                     ARARQRLPYGVSAYIVQQLAKGLDYAHRKTDEFGQPLGIVHRDVSPQNILLSWDGSVK
                     IVDFGIARARDVHEEEGVIKGKYAYMSPEQARGEPVDCRSDVFAAGIVLFELACARPL
                     FHGKGKEALEMVKSGVLPRPRDYAPDLPEALERVILRALAFRREDRFATARDLQHELG
                     KFQLAWGNKIGALIDSGSLAQTLAQLVPHERASAPRPPAEPDPDGRSAIDPGSGEIG"
     gene            complement(1082..1702)
                     /locus_tag="VF516_19060"
     CDS             complement(1082..1702)
                     /locus_tag="VF516_19060"
                     /inference="COORDINATES: protein motif:HMM:NF023669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109842.1"
                     /translation="MHRWLAIGLVLAACKVREQPPITAAWTWDFDAGLGDVFYATGDG
                     YHGERGALSAHGAHNHPLWLRKKLPRDVRIEFDAWSTEPRGDIKVEVFGDGHSFDPDG
                     GRYTATGYEVIFGGWFNSRSILARLDEHGTEMAERTEPKVVPGRHYHWRIERRGKAIT
                     WFLDDLATPFLRYEDPRPLDGAGHEYFGFNNWETDTWFDNLVIAPL"
     gene            1824..2336
                     /locus_tag="VF516_19065"
     CDS             1824..2336
                     /locus_tag="VF516_19065"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEX8109843.1"
                     /translation="MEGTLSLFDWRPAPVVPDVPERMPEPVEDVAAGSGPCAGPDPVQ
                     QAHPGARLVIFDCETTGTDRLTDQVIELCIQLGLGDDASSRTWRIKPTAPIHPGAQAV
                     HGITLADLEGCPAFAEVAEDIAAVFAETDVIVGYNIAFDIDMLQAEYTRIGKPMLDLT
                     GKKVVDAFRL"
     gene            2442..2660
                     /locus_tag="VF516_19070"
     CDS             2442..2660
                     /locus_tag="VF516_19070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109844.1"
                     /translation="MLSAMLHAFNLADHDWDAIAGKCDPQHNARASWVGPSRHLRWDG
                     EVVVLGFGKYANTAVHQLAAGPDRSFLR"
BASE COUNT          397 a          946 c          922 g          398 t
ORIGIN      
        1 ccgatctcgc ccgagccagg atcgatcgcg gagcggccgt cggggtcggg ctcggccggg
       61 gggcgcggcg cgctcgcgcg ctcgtgcggg accagctgcg cgagggtctg ggcgagcgat
      121 ccggagtcga tcagcgcgcc gatcttgttg ccccacgcga gctggaactt gccgagctcg
      181 tgctgcagat cgcgcgcggt cgcgaaccgg tcctcgcggc ggaacgcgag cgcgcgcagg
      241 atgacgcgct ccagcgcctc gggcaggtcg ggcgcgtagt cgcgcggccg cggcagcacg
      301 cccgacttca ccatctcgag cgcctccttg cccttgccgt ggaacagcgg ccgcgcgcac
      361 gccagctcga acagcacgat gccggcggcg aacacgtcgc tgcggcagtc gaccggctcg
      421 ccgcgcgcct gctccggcga catgtacgcg tacttgccct tgatcacgcc ctcctcctcg
      481 tgcacgtcgc gcgcgcgggc gatcccgaag tcgacgatct tcacgctgcc gtcccacgac
      541 agcaagatgt tctggggcga cacgtcgcgg tgcacgatgc cgagcggctg gccgaactcg
      601 tcggtcttgc ggtgcgcgta gtcgagcccc ttggcgagct gctgcacgat gtacgccgac
      661 acgccgtagg gcaggcgctg gcgggcgcgg gcggcctcct gcagcaggcg cagcaggtcg
      721 acgccctcga cgtactccat cgcgaggaag taggtgtcgg cgaccgcgcc gaagtcgaac
      781 acctgggtga cgttcgggtg gttgaggccg agcgcgatct tggcctcgtc gacgaacatc
      841 gcgacgaact ggcggctgcg cgcgtaggcg gagtggatct tcttgatcac cagcgtcttg
      901 ttgagcccct gcgcgacggt ggtccgcgcc aggaacacct cggccatgcc gccggtcccg
      961 atcttgcgga tcagcgaata tttaccgaac tgctccatgc cggggggcca acgaccgggg
     1021 ccaacgacgc gacgaccggc gcggggacac taccaccgag ccggtccggg ccattgtagc
     1081 gtcacagcgg cgcgatcacg aggttgtcga accaggtatc ggtctcccag ttgttgaagc
     1141 cgaaatactc atggccggcg ccatcgagcg gccgcgggtc ctcgtagcgc aagaacggcg
     1201 tcgcgaggtc gtcgaggaac cacgtgatcg ccttgccgcg gcgctcgatc cgccagtggt
     1261 agtgccggcc gggcacgacc ttgggctcgg tgcgctccgc catctcggtg ccgtgctcgt
     1321 cgagccgcgc gaggatcgac ctggagttga accagccgcc gaagatcacc tcgtagccgg
     1381 tcgcggtgta gcggccaccg tcggggtcga acgagtggcc gtcaccgaac acctcgacct
     1441 tgatgtcgcc gcgcggctcg gtcgaccacg cgtcgaactc gatccggacg tcgcgcggca
     1501 gcttcttgcg cagccacagc ggatggttgt gcgcgccgtg cgccgacagc gcgccgcgct
     1561 cgccgtggta gccgtcgccg gtggcataga acacatcgcc gagcccggcg tcgaagtccc
     1621 acgtccacgc cgccgtgatc ggcggctgct cgcggacctt gcaggcggcg agcacgaggc
     1681 cgatcgcgag ccagcggtgc atcgagccgg catcgtacgc catcgggtgc gatctcggtg
     1741 aaccctaacg tattcgatcg cttgcgttct tcgccgatct ccgaaggtga ttcgctgtca
     1801 gaccgctgcc ctagcgtggc ggcgtggaag gtacgctgtc gctgttcgac tggcgcccgg
     1861 cgccggtggt gccggacgtc cccgagcgca tgccggagcc cgtcgaggac gtcgcggcag
     1921 ggagcggccc atgcgcgggg ccggatccgg tgcagcaggc gcatcccggc gcgcgcctcg
     1981 tgatcttcga ctgcgagacc accggcaccg accggctcac cgatcaggtg atcgagctgt
     2041 gcatccagct cggcctcggc gacgacgcgt ccagccggac ctggcggatc aagccgaccg
     2101 cgccgatcca ccccggcgcc caggccgtcc acggcatcac cctggccgac ctcgagggct
     2161 gcccggcgtt cgccgaggtc gccgaggaca tcgcggcggt gttcgccgag accgacgtga
     2221 tcgtcggcta caacatcgcg ttcgacatcg acatgctgca ggccgagtac acccggatcg
     2281 gcaagccgat gctcgacctc accggcaaga aggtcgtcga cgcgttccgg ctgtgacagc
     2341 agtgcgagcc gcgcagcctg cagcacgccc accagcgctt cgtcggcatc ggcttcgagg
     2401 ccgcgcacag cgccagcgcc gatgtcgctg cgaccggccg cgtgctgtcc gcgatgctgc
     2461 acgcattcaa cctcgccgat cacgactggg acgcgatcgc gggcaagtgc gacccccagc
     2521 acaatgcgcg cgccagctgg gtcggcccat cgcgccacct gcgctgggac ggcgaggtcg
     2581 tcgtgctcgg cttcggcaag tacgccaaca cggccgtcca ccagctcgcc gccggccccg
     2641 atcgcagctt cctgcgctga gtc
//
LOCUS       DASVOR010000987         3839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_39255,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000987 DASVOR010000000
VERSION     DASVOR010000987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39255"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VF516_19075"
     CDS             <1..396
                     /locus_tag="VF516_19075"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8109845.1"
                     /translation="KITMLAGYYVLHKYRGHYYAKAQNLARKVTAAYDAALARYDALL
                     LPTTPIKATRLPAPDAPRAEILARGFEMIANCAPFDVSGHPALTMPCGLSDGLPAGLM
                     LVGKQFDEMTLYRLASAWESGADGKSIRP"
     gene            479..1096
                     /locus_tag="VF516_19080"
     CDS             479..1096
                     /locus_tag="VF516_19080"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8109846.1"
                     /translation="MAYEYDIFLSYKRHPESRQWLVEHFQPLLEFHVELELGRKVTVF
                     RDDQDDEAGTSWPAHLGRALGNSRTLVALWTRTYFHSEWCSRELSTMLARERDERFRT
                     AERPEGLIFPVVLHDCEQLPPKLPPMQFVAMQECFQVRMARNSQTAEILAQQISKRLA
                     PGVASAVAAAPRWRAKWPLKTAGKFLAAVRRKGPPRQTQLPRHSR"
     gene            1531..3276
                     /locus_tag="VF516_19085"
     CDS             1531..3276
                     /locus_tag="VF516_19085"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8109847.1"
                     /translation="MLIDSRTGMSDAGGICTIQMPDVLVTMFTANYQSVFGVRDVIQS
                     AQSARQRLAYDRMALTVLPLPSRFPHGGDSKLSNEWLDRIATELGACLADWLPRGIDP
                     RSVLERLAIPQRDEFALGERLAVAEQKTAGAEALTAVYDRIAALLAAEFRDIEAALGP
                     IEPSSMSRSVAWALEKPAHGPSKLPVENDVFVSYPRAGSVLSEWTYGFIVALMKAFTA
                     LGRSASFYIDLEDRADAEFDRLRPILDRSRVLLAILTPHYLVSSWCLAEWQAFEDREQ
                     TEGRTLIVPVALRGVAALPGRFSERVVVDGSGTDLADTSDAAMATLVDRTVKALVATL
                     EGRSSPKRGPDPQSAPIDTRWSTKVGRARYPILLLAANPLSAGQLALQVEAKEIWRAI
                     DASQFRARFQLDLSWNVKLDELLGYLRNLNPAVVHFSGQGASDGVYLVGRDGLPQLVP
                     GAVLAQLIRAAGSDLRLVVLSGCYSEKQAAALSAVVDCVVGVVASISDEATVAFSAAF
                     YAALASGRSVASACQQGRAAILLRAASSERWSKDSGETIRLQTRPGVRADAVSFATQP
                     RPPKAPPGKRRVSTR"
     gene            complement(3335..3655)
                     /locus_tag="VF516_19090"
     CDS             complement(3335..3655)
                     /locus_tag="VF516_19090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8109848.1"
                     /translation="MFDALEMSLTVLEQLAPVETKVRQRRKSLADEIGRAAESVALNV
                     SEARQRAGLDRADLFRRAAGSASELTTALRIARARGYITPAEHAAVDAALDRVRAMLW
                     RLTH"
BASE COUNT          576 a         1291 c         1355 g          617 t
ORIGIN      
        1 aagatcacga tgctcgccgg ctactacgtg ctccacaagt accgcggcca ctactacgcc
       61 aaggcccaga acctggcgcg caaggtgacc gcggcgtacg acgccgcgct ggcccgctac
      121 gacgcgctgc tgctaccgac cacgccgatc aaggcgacca ggctgcccgc gcccgacgcg
      181 ccgcgcgccg agatcctggc ccgcggcttc gagatgatcg ccaactgcgc gccgttcgac
      241 gtcagcggcc acccggcgct gaccatgccg tgcggcctga gcgacggcct gccggcgggc
      301 ctgatgctgg tcggcaagca gttcgacgag atgacgctgt accggctggc cagcgcgtgg
      361 gagtccggcg ccgacggcaa gtcgatccgg ccgtgaccgg cgattccgcg gaatcgcgcc
      421 ccgcgtagca ggacggcgcg ccgcggcggt ctgttatgct gggcgcgcgg gcagggccgt
      481 ggcctacgag tacgacatct tcctgagcta caagcgccat cccgagtcga ggcagtggct
      541 ggtcgagcac ttccagccgc tgctcgagtt tcatgtcgag ctcgagctcg gccggaaggt
      601 gaccgtgttc cgtgacgatc aggacgacga ggcgggcacg agctggccgg cccacctcgg
      661 gcgggcgctc ggcaactcgc gcacgctggt ggcgctgtgg acccggacgt acttccacag
      721 cgagtggtgc agccgcgaac tatcgacgat gctcgcgcgt gaacgcgatg agcggttccg
      781 gacggccgaa cgccccgagg gcctgatctt cccggtcgtg ctgcacgatt gcgaacagct
      841 gcctccaaag ctgccgccga tgcaattcgt ggcgatgcag gaatgctttc aggtccggat
      901 ggcacgtaac agtcagaccg cggagatcct cgcgcagcag atctcgaagc ggcttgcgcc
      961 cggcgtcgcc tccgcagtcg cggccgcgcc gagatggcgg gcgaagtggc cgctcaagac
     1021 ggccgggaag ttcctcgccg ccgtcaggcg caaggggccc ccacgccaga cccagctccc
     1081 gaggcattcg cggtgagcca gatcgtcacg ttctattcgt acaagggcgg cgtcggccgc
     1141 agcatggcgc tcgccaacat cgcggtgctg ctcgcgcggc gcggtctccg cgtgctctcc
     1201 gtcgactggg atctcgaggc gccgggcctc gagcgctact tcgactactt cgagctctcg
     1261 atcaagcgcg gcgggctgct gccgttcctg gtcgagcagc acgcccagct gggcgccgac
     1321 ggcgaggcca acgcggacca ctaccgccac cacctgtgga cgatcgatgt cggcggtgcg
     1381 caccggctat cgctgctcgg cagcggccgt gcggcccacg ccgactacac gcgcgtcctc
     1441 gagcacttcg cgtggagcgc gttcttccag cgcggcggcg gcgatttcct cgagcagctg
     1501 cgcgatcgat ggcgcgccga cttcgacgtc gtgctgatcg acagccgcac cgggatgagc
     1561 gatgcgggcg gcatctgcac gatccagatg cccgacgtcc tggtcacgat gttcaccgcg
     1621 aactaccaga gcgtgttcgg cgtgcgcgac gtcatccagt cggctcagtc ggcccgccag
     1681 cgcctggcgt acgaccgcat ggcgctgacg gtcctccctc tgccgagccg gttcccacac
     1741 ggtggcgaca gcaagctgtc gaatgaatgg ctggaccgga tcgccaccga gctcggcgcc
     1801 tgtctcgcgg actggctccc gcgcgggatc gatccgcgca gcgtgctcga gcggctagca
     1861 atcccgcaac gcgacgagtt cgcgctcggc gagcgtctcg ccgtcgcgga gcagaagacg
     1921 gccggggccg aggcgctgac cgccgtctac gaccggatcg cggcactgct cgcggccgag
     1981 ttccgggaca tcgaagccgc gctgggcccg atcgagcctt cttcgatgag ccgatcggtc
     2041 gcatgggcgc tcgaaaaacc cgcccatggc ccctccaagc ttcccgtcga gaacgatgtg
     2101 ttcgtgagct atccgcgggc cggcagcgtg ctcagcgaat ggacctacgg gttcatcgtg
     2161 gcgctgatga aggcgtttac cgcgctgggg cggtctgctt cgttctacat cgatctcgag
     2221 gaccgggccg atgccgagtt cgaccgactg cgacccatcc tggaccgatc tcgcgtcttg
     2281 ctggccatcc tgacgccgca ctatctggtg tcgtcgtggt gcctcgccga gtggcaagcc
     2341 ttcgaggacc gcgagcaaac ggagggacgg acgctgatcg tgccggtcgc gctgcgcggg
     2401 gtggcggcgc ttcccggccg gttcagcgaa cgcgtggtgg tggacggctc cggcacggac
     2461 ctggcggaca cgagcgatgc agcgatggcg acgctcgtgg accggaccgt gaaggcgctg
     2521 gtcgcgacgc tcgaaggccg gtcgtcccca aagcggggtc cggatccaca atccgcaccc
     2581 attgatacgc gctggtcgac caaggtcggt cgggcgcgat acccgatcct gctcctcgct
     2641 gcgaatccgc tgagcgcggg acagctggcg ctgcaagtcg aggcgaagga gatctggcgt
     2701 gcgatcgacg catcgcagtt tcgagctcgg ttccagttgg acttgagctg gaacgtgaag
     2761 ctggacgagc tgctgggcta cctgcgtaac ctcaatcctg ccgtggttca cttcagcggt
     2821 cagggtgcca gcgacggcgt gtacctggtg ggtcgggacg gtcttccgca gctcgtgccg
     2881 ggggcagtgc ttgcacagct gatccgtgcc gcgggcagtg acctccggct ggtcgtgctg
     2941 agtggttgct acagcgagaa gcaggccgcg gcgttgagtg ccgttgtcga ctgtgtggtc
     3001 ggcgtggtcg cgtcgatcag cgacgaggcc accgtcgcgt tctcggccgc attctatgcg
     3061 gcgctggcgt cgggccgatc cgttgctagc gcctgtcagc agggtcgagc cgcgatcttg
     3121 ctgcgagccg cgagctcgga gcgctggagc aaagactccg gggagacgat tcgattgcag
     3181 acccggccgg gtgtgcgcgc ggacgctgtg tccttcgcga cgcaacccag gcccccgaag
     3241 gccccccccg gcaagcgccg agtcagcacc cgctgagcta gtgatccccg ggattgtcgt
     3301 ggcgcgatgg agcccgagac agcccgggcg gtgctcagtg ggtgagccgc cacagcattg
     3361 cccgtacccg atccagtgca gcgtcgaccg cggcgtgttc ggccggggtg atgtagccgc
     3421 gcgcgcgagc gatgcgcagc gccgtggtga gttcgcttgc cgagccggca gcgcggcgga
     3481 acaggtcggc gcgatcgagg ccggcgcgct gccgcgcttc gctgacgttg agggccaccg
     3541 attcggcggc gcggccgatc tcgtcggcga gtgatttgcg gcgctggcgg accttggtct
     3601 ctaccggagc gagttgctcc agcacggtga gcgacatctc gagcgcatcg aacatgcgaa
     3661 cctccttgtg attcgccccc cgcatcgggg gcgtcgtcgc ccgacgcgcc gcggagcgca
     3721 cgtcccatga cgagcgtggg caccgcgatt cgcgtgcgtc gatcgatcca gctgtggggg
     3781 caccgttcgc gtcacgtcgg tcggtccggc gctcggcaag ggacggtgcg cgagagcgg
//
LOCUS       DASVOR010000988         7915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_360750,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000988 DASVOR010000000
VERSION     DASVOR010000988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360750"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VF516_19095"
     CDS             <1..532
                     /locus_tag="VF516_19095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109849.1"
                     /translation="HEVNLKSNNWWRGRLRSAYYFHEDFARSNDLEAALRRVTSAHIK
                     ASAARFLDENNLIVGILRPAADAAPATKPVPIAVPPGTGATAADDARALRSTRCCPMS
                     WARLLPPARSRTCAARRRRERQAPSRGRSRRRAARRAGIGVGPASDASRVGEGDPVVT
                     GDAMPDQGAKLHAPML"
     gene            743..4114
                     /locus_tag="VF516_19100"
     CDS             743..4114
                     /locus_tag="VF516_19100"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8109850.1"
                     /translation="MLTQHVDSARTGWNPAETQLTLQSVGQQSFGKLFTVPVIGRIFA
                     HPLFVPQVSRADGTHDIVVVATEANWVYGIDGQSGAVLWQRNLGTPIDSTAAYPQQCM
                     NIPGTIGITGTPVIDPATGTIYLVAANQQPGPVYQQFLHALDVATGVPRVTARQITAQ
                     VLGDGPAAVNGLISFDALRANQRAGLLLLGGQIYVAWGGHCDDAVDAINHPGSKQIFF
                     GWIMSFDPTTLARTGAFNTAPRADPNPNEQIPYGGSIWQSGGGLASDGNRIYLNTGNA
                     LFNPSIGSWGDSTLAFTPSLGLASTFTPFDQAFLDNSDLDLSAGGVVIVPGGVAAHAN
                     LLVTTGKEGTIYVLDRNALGGAQPAAGDVVCPAGSAPGNCVVGELSRAVGGPGTVPNP
                     GGYFGIPAYFNGQVYFAGNADQMKSFTVGGASAIAGPTHTTTTIFNYPGSTPSVSSNG
                     TADGIVWALERTQPGPFQFPDPTYLHAYTADTLAELYNSHLNDDDPDGAGQFMAPTIA
                     GGRVYVGTSSSLAVYGLTIRVTPGSQSVVPGSSTTYTVVTAAIQGLADTVTFSVSGLP
                     AGTSASFSPASVPRGGTATLTLTTTTATPVGTFSFTVAATGPSRTVSATAGLTVAQRP
                     VAAFTSSCSGRTCSFDGSASQAFQGRTIVSYAWSFSDGGTASGATPDPHHYPGDGSFQ
                     ATLTVTDSGGLSSQPLTQTVTVSDQPPHAGFSVVCIDRTCFPDAETSNDDVGITSYSW
                     NWGDGSAPSTGSNLSETSHVYAAYGAYTITLTVSDVENHTATDSHPVALGRPPTASFT
                     IACTGRICDVDASASTGDVPIASYHWDWDDETATDSGAPTAHHVYASDDTFAVHLRVT
                     DINGNFGDLTKSVTVADAPPHAGFSFVCIDRACFFDPETTSDDIGVTSFTWDWGDGTT
                     TSGIGAVAAIEHDFAYGTYAVKLTVVDIDSQTDAVTQTVTVVPAPVAAFTASCSGLLC
                     SFDASASTSAAAITSYHWDWDDETALDANVATAQHRYAFGDTFRVHLRVTDATGHTAD
                     VTHSVTATAPPPGVVASFTFTCSGRTCQFDASGSTSSAAIVNYHWDWDDETTTDATSA
                     AAQHVYAWSQTFLVHLAVTDSSGQIGRITRSVVVH"
     gene            complement(4363..4554)
                     /locus_tag="VF516_19105"
     CDS             complement(4363..4554)
                     /locus_tag="VF516_19105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109851.1"
                     /translation="MTDRGLAVSVRSAPPRALVSTIAAAPVRARCSHAIAVPPGDQRG
                     FCSRSGSAVVLPVARSTSW"
     gene            4885..5499
                     /locus_tag="VF516_19110"
     CDS             4885..5499
                     /locus_tag="VF516_19110"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8109852.1"
                     /translation="MAYFRPDPRGSDGYGDWSYKAIVGNSGDGPMVDDLAGVDAVLAS
                     GVGDKDRLFIEGGSYGGYLTSWIITHDQRFKAAVAAVPVTNLLLDYTLSESPNITRRF
                     YGARPALDPALLSRESPITYAGAAHTPLLVEIGLLDTRAPYVQAIEFYKVVAETGTEA
                     RLLVDTQSGHGPDDPRGILQWQQAMLGWFAAHGGPAIPGAVLPK"
     gene            complement(5391..6248)
                     /locus_tag="VF516_19115"
     CDS             complement(5391..6248)
                     /locus_tag="VF516_19115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109853.1"
                     /translation="MLEAEVTPARRRPWRIVALLGLVLAGCVLVAFVLARDPEEPVVP
                     ILVPAPPPVPAPTPGPAANDPHVDEPIESIAAASDAPVIAVASAHHVWISRDDGNTFA
                     PALQDPGAEVYDVTVEPSGRVLAMWGENKRWRSPGGGGVDTIEFALGAAELDGRERWR
                     PMNEMIAAPLDARAGWVVGSGVPVIGRDSGESWKRVPSSGRWHVWRASIDDLHTARFF
                     GDRPAKDALLRSSDSARCRRARSRAAHRARCRHFGSTAPGIAGPPCAANHPSMACCHW
                     RMPLGSSGP"
     gene            6357..7130
                     /locus_tag="VF516_19120"
     CDS             6357..7130
                     /locus_tag="VF516_19120"
                     /inference="COORDINATES: protein motif:HMM:NF012995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HEX8109854.1"
                     /translation="MDLDRYFERIGWRGARGAGDDVLAGLYAHHLRAIPFENLDVLLG
                     RRVQIDLPALEAKLVHARRGGYCYEHATLFAAVLRALGFEVHTHSARVVMVRSRDEAP
                     RTHMFLTSGRWLLDIGFGGPAPLSPVLLDGTPSGDHRFVRDGADHALEARAADGSWRR
                     LWVSSLERDILIDFEMANHFTSTHPSSPFTQRLMGQAFTPAGKVAVANRDVTIIEHGQ
                     TRTSVLADRDELRALFAAQFGFDLPELARVKIPAIPEWP"
BASE COUNT         1217 a         2872 c         2677 g         1149 t
ORIGIN      
        1 gcacgaggtc aacctcaaga gcaacaactg gtggcgcggc cggctgcgca gcgcgtacta
       61 ctttcacgag gacttcgcgc gcagcaacga cctcgaagcc gcgctgcgcc gcgtgaccag
      121 cgcccacatc aaggccagcg ccgcccggtt cctcgacgag aacaacctca tcgtcggcat
      181 cctgcgcccc gcggccgacg ccgcaccggc gaccaagccg gttccgatcg cggtgccgcc
      241 cggcaccggc gcgaccgccg ccgacgacgc gcgcgcgctg cgctccacgc gctgctgtcc
      301 gatgagctgg gcgaggctgc taccaccagc tcgatctcga acgtgcgcag cgcgccggcg
      361 tcgcgaacgg caagcgccgt cgcgaggtag aagtcggcgg cgtgcagctc gtcgagcggg
      421 catcggggtc ggcccggcca gcgacgcgtc tcgcgtaggc gagggcgacc ccgtcgtcac
      481 cggggacgcc atgcccgatc agggcgcgaa acttcacgcg ccgatgttat gatgccggcg
      541 cagccgctgc cagcagctcg tgcgagcggc cggagatcga atcgacgctc caggagagac
      601 aatgtcgccg tacgtacgtg tgtcaaggat ccgtcatgtg gcttctgccg tgctctgtgc
      661 ggcgctcgcc tcctgcagcg cggagagtcc agaccgactg caggagcgga cgtccgcctc
      721 gaccctcatc accaccggtg cggtgctgac ccagcatgtc gacagcgcgc gcaccggctg
      781 gaaccccgcc gagacccagc tcaccctgca gagcgtcggc cagcagagct tcggcaagct
      841 gttcaccgtg ccggtgatcg gccggatctt cgcgcacccg ctgttcgtcc cgcaggtgtc
      901 gcgcgccgac ggtacccacg atatcgtggt ggtcgcgacc gaggccaact gggtctacgg
      961 catcgacgga cagagcggcg ccgtgctgtg gcagcgcaac ctcggcacgc cgatcgattc
     1021 gaccgccgcc tacccgcagc agtgcatgaa catcccgggc acgatcggca tcaccgggac
     1081 gccggtgatc gatccggcga ccgggacgat ctacctggtg gccgcgaacc agcagccagg
     1141 cccggtgtat caacagttcc ttcatgcact cgacgtcgcg accggcgtcc cgcgggtgac
     1201 ggcgcggcag atcaccgcgc aggtgctggg cgacggtccg gccgcggtca acggcctgat
     1261 ctcgttcgat gcgctgcgcg ccaaccagcg cgcgggtctg ctgctgctcg gcgggcagat
     1321 ctacgtcgcg tggggcggcc actgcgacga cgccgtcgat gcgatcaacc accccggcag
     1381 caagcagatc ttcttcggct ggatcatgag cttcgatccg accacgctgg cgcggaccgg
     1441 cgcgttcaac accgcgccgc gcgccgatcc caacccgaac gagcagatcc cgtacggcgg
     1501 cagcatctgg cagagcggcg gcgggctcgc gtccgacgga aatcgcatct acctcaatac
     1561 cgggaatgcg ctgttcaatc cctcgatcgg gagctggggc gatagcacgc tggccttcac
     1621 gccgtcgctc gggctggcca gcacgttcac gccgttcgac caggcgttcc tcgataacag
     1681 tgacctcgac ctgtcggcgg gcggtgtggt gatcgtcccc ggcggcgtcg ccgcgcacgc
     1741 caacctgctg gtcaccaccg gcaaggaggg cacgatctac gtgctcgatc gcaacgcgct
     1801 cggcggcgcg cagccggcgg ccggtgatgt cgtgtgcccg gccggctcgg cgccgggcaa
     1861 ctgcgtggtc ggagagctgt cccgcgcggt cggtggcccc gggacggtcc ccaaccccgg
     1921 tggctacttc ggcattcccg cgtacttcaa tgggcaggtc tacttcgcgg gcaatgccga
     1981 tcagatgaag tcgttcacgg tcggcggtgc atcggcgatc gcaggcccga cccacaccac
     2041 gacgacgatc ttcaactacc cgggatcgac gccatcggtg tcgtcgaatg gaaccgccga
     2101 cggcatcgtc tgggcgctcg agcgcacgca gcccggtccg ttccagttcc ccgacccgac
     2161 ctacctgcat gcctataccg cggacacgct ggccgagctc tacaatagcc atctcaacga
     2221 tgatgacccc gacggtgcgg ggcagttcat ggcgcccacc atcgccggcg gccgggtcta
     2281 cgtcggcacc agcagctcgc tcgcggtgta cggcctcacg atccgcgtca cgccggggag
     2341 ccagagcgtc gtgccgggat cgagcacgac ctacacggtc gtaaccgcgg cgatccaggg
     2401 cctcgccgat acggtcacgt tctccgtgtc agggttgccc gccgggacct cggcgtcgtt
     2461 ctcacccgcg tcggtgccgc gcggcggcac cgcgacgctc accctcacca cgaccaccgc
     2521 gaccccggtc ggcaccttct cgttcaccgt cgcagccacc ggcccgagcc gcacggtgtc
     2581 ggcgaccgcg gggctgaccg tggcgcagcg cccggtcgcc gcgttcacgt cgtcgtgcag
     2641 cggccgcacc tgcagcttcg acggctcggc ctcgcaggca ttccagggcc gcacgatcgt
     2701 gtcctacgcg tggagcttca gcgacggcgg cacggccagc ggggccaccc cggacccgca
     2761 tcactatccg ggcgacggca gcttccaggc caccttgacg gtgaccgaca gcggcgggct
     2821 cagcagccag ccgctgaccc agacggtcac ggtgtccgac cagccgccgc acgccgggtt
     2881 ctccgtggtc tgcatcgatc ggacctgctt ccccgacgcc gagacctcga acgacgacgt
     2941 cggcatcacc agctactcgt ggaactgggg cgacggcagc gcgccgagca ccggcagcaa
     3001 tctcagcgag acgtcgcacg tgtacgccgc gtacggtgcg tacacgatca cgctgacggt
     3061 gagcgacgtc gagaaccaca cggcgaccga cagccacccg gtggcgctgg gccggccgcc
     3121 gaccgcctcg ttcacgatcg catgcacggg ccggatctgc gatgtcgatg cgtcggcgtc
     3181 gaccggcgac gtgccgatcg cgagctacca ctgggactgg gacgacgaga cggccaccga
     3241 ttccggcgcg ccgaccgcgc atcacgtgta cgccagcgac gacacgttcg cggtgcacct
     3301 gcgcgtgacc gacatcaacg gcaacttcgg cgacctgacg aagtcggtga ccgtggccga
     3361 cgcgccgccg cacgcgggct tctcgttcgt gtgcatcgat cgcgcgtgct tcttcgatcc
     3421 cgagaccacg agcgacgata tcggcgtcac cagcttcacg tgggactggg gggacggcac
     3481 gacgacgtcg ggcatcggag cggtcgccgc gatcgagcac gacttcgctt acggcacgta
     3541 tgcggtcaag ctcaccgtcg tcgacatcga cagtcagacc gatgcggtga cgcagaccgt
     3601 gacggtggtg cccgcgccgg tcgctgcgtt caccgccagc tgcagcggcc tgttatgcag
     3661 cttcgacgca tcggcgtcga ccagcgccgc ggcgatcacc agctatcact gggactggga
     3721 cgacgagacc gcgctggatg cgaacgtggc cacggcgcag catcgctatg ccttcggcga
     3781 tacgttccgt gtccacctgc gggtcaccga cgccaccggc cacaccgccg acgtgaccca
     3841 cagcgtgacc gcgacggcgc cgccgcccgg cgtggtcgcg tcgttcacct ttacgtgcag
     3901 cggacgcacc tgccagttcg acgcctccgg ctcgacgagt agcgccgcga tcgtcaacta
     3961 ccactgggac tgggacgacg agaccacgac ggacgcaacc tccgcggcag cgcagcacgt
     4021 ctatgcatgg tcgcagacgt tcctggtcca tctcgcggtg accgactcgt cgggtcagat
     4081 cggccgcatc acgcggtcgg tcgtcgtgca ttgaccggcc gccgaccatg ctcgagggac
     4141 ggaggaaccg ccatgatcgc tcgatctctg gccgcgggtg tcgggctcgc cgccgcgctt
     4201 gcgcctcctg ctcgaaccgc ggccgcgcag ggccgcccgt ggacgctcac cgatgcggtg
     4261 caggtcgagg ccatcgacga cgtccagatc gcgcccgacg gcagcgtcgc gctgatcgcg
     4321 gtcacgtcgc gccgacgtcg cgcggaacgt gttcgcccgg gcctaccagc tcgtcgacct
     4381 ggccaccggc aagacgaccg cgctgcccga gcggctgcag aacccgcgct ggtcgcccgg
     4441 cgggacggcg atcgcatggc tgcagcgcgc gcggaccggc gcggcggcga tcgtgctcac
     4501 cagcgcccgc ggcggggcag agcggacgct gacggcgagc ccgcggtcgg tcatggcgtt
     4561 ccgctggtcg gccggacggc aagcggatcg ccgcggtcga ggcagccgcg gcccccgccg
     4621 ccgcggacca ggcacggttc cgctggatga cgcccgagag cgactatcgc gacacccgtc
     4681 cgagcaagcg cgacgtctac gtgatcgacg tggagcggcg gaccgaggcc ctgctgaccc
     4741 acgacagctg gtcgtacggc gggccggtca ccgatcaccg gcgatccgcg cgccgcggcc
     4801 ctcgtcgtcg agccgcacgg cgggccccag tgcgcggacg acgcgtcgtt ctctggcttc
     4861 gcgcagtacc tggcgaccaa cggcgtggcg tacttccgcc ccgatccgcg cggcagcgac
     4921 ggctatggcg actggtccta caaggcgatc gtcggcaaca gcggcgacgg gccgatggtc
     4981 gacgacctcg ccggcgtcga cgccgtgctg gccagcggcg tgggagacaa ggatcgcctg
     5041 ttcatcgagg gcgggtcgta cggcggctac ctcaccagct ggatcatcac gcacgatcag
     5101 cggttcaagg ccgccgtcgc ggcggtgccg gtgaccaacc tgttgctcga ctacacgctg
     5161 tccgagagcc ccaacatcac gcgccggttc tacggcgccc ggccggcgct cgatccggcg
     5221 ctgctgtcgc gcgagtcgcc gatcacgtac gcgggcgcgg cgcacacgcc gctgctcgtg
     5281 gagatcggcc tgctcgacac gcgcgcgccg tacgttcagg cgatcgagtt ctacaaggtg
     5341 gtcgcggaga ccggaaccga ggcccggctg ctggtcgaca cgcagtccgg tcacggtccg
     5401 gacgatccca ggggcatcct ccagtggcag caggccatgc tcggatggtt cgcggcgcac
     5461 ggcggacccg cgatcccggg cgccgtgctg ccgaagtgac ggcagcgagc gcgatgcgcc
     5521 gcgcgagatc tcgccctcct gcaacgcgcg ctatcggatg aacgcaacaa ggcgtccttc
     5581 gccggacggt caccgaagaa ccgcgcggtg tggaggtcgt cgatcgaggc ccgccacaca
     5641 tgccacctgc ccgacgaggg aacgcgcttc cacgactcgc cggagtcccg tccgatcacg
     5701 ggcacgcccg agccgacgac ccagcctgcg cgcgcgtcga gcggcgcggc gatcatctcg
     5761 ttcatcggtc gccagcgctc gcgaccgtcg agctcggcgg ccccgagcgc gaactcgatg
     5821 gtgtcgactc cgcccccgcc gggagagcgc caccgcttgt tctcgcccca catggccagg
     5881 acgcgccccg agggctcgac cgtcacgtcg tagacctcgg ctcccggatc ctggagcgcg
     5941 ggcgcgaacg tgttgccgtc atcgcggctg atccagacgt ggtgcgccga cgccaccgcg
     6001 atcaccggcg cgtcgctcgc cgcggcgatg gattcgatcg gctcgtcgac gtgcggatcg
     6061 ttcgccgccg gcccgggcgt cggcgcgggc acgggcggcg gcgcgggcac gaggatcgga
     6121 acgacgggct cctccggatc ccgcgcgagc acgaaggcca cgagcacgca gcccgcgagc
     6181 acgagcccga gcagcgcaac gatccgccac gggcgacgac gagctggggt gacctccgct
     6241 tcgagcatgc cgctcccggt gcaacgaccg agccgccgcc gctattccct ggcgggctcg
     6301 tggaggcaca ccccgcgcca tcgaaaaccg cacgcggtac ccctatgatg cgtggggtgg
     6361 atctcgacag gtatttcgaa cgcatcggct ggcgaggcgc acgtggcgcc ggcgacgacg
     6421 tactcgcggg gctctacgcg catcacctgc gcgcgatccc gttcgagaac ctcgacgtgc
     6481 tcctcggccg gcgcgtgcag atcgacctgc ctgccctgga ggcgaagctc gtgcacgcgc
     6541 gtcgcggcgg ctactgctac gagcacgcga ccctgttcgc cgcggtgctg cgcgcgctcg
     6601 gcttcgaggt ccacacccac agcgcgcgcg tcgtcatggt gcgatcgcgc gacgaagccc
     6661 cgcgcacgca catgttcctg acctcgggcc ggtggttgct cgacatcggc ttcggcgggc
     6721 cggcgccgct gtcgccggtg ctgctcgacg gcacgccgag cggcgaccac cggttcgtgc
     6781 gcgacggcgc cgatcacgcc ctcgaggcac gcgccgccga cggcagctgg cgacggctgt
     6841 gggtgtcgtc gctcgagcgc gacatcctga tcgacttcga gatggcgaac catttcacgt
     6901 cgacgcatcc gagctcgccg ttcacgcagc gcctgatggg gcaggcgttc acacccgctg
     6961 gcaaggtcgc ggtcgcgaac cgcgatgtca cgatcatcga gcatggccag acgcgcacgt
     7021 cggtgctcgc cgatcgcgac gagctgcgcg ccctgttcgc ggcgcagttc gggttcgatc
     7081 tgcccgagct cgcgcgcgtg aagattccgg cgatcccgga gtggccctga ccgctcagtc
     7141 gtcgccgggg gtcgccgtcc cggtcactgc gcttcacaga atccgtagtt gcacgccggg
     7201 gtcgccccac cgcagtcgcc ggctccggcg cacgacttgc agcgcgcggg cgcggcgccg
     7261 ttgttgacct gcttctcgaa gaagcagccc agccgaacga cggcttgtac aagctgagcg
     7321 gcccgacctc ggtggtatgg gtgaccgtca acgcgcacgg tgggacgaag ccggccgcga
     7381 tgatcgcggt cagcacttgc tgctggagca tcggcgccac gagcgtcgtc ggagatcccg
     7441 gtcgtggcgc tgtagccgca cgacgacggg tacacgtcgg actcgccgac atccacggtg
     7501 ttgccgttcg tggcgtcgag caggcagaac gtctgcttgc cggtggccgc gacggcgaga
     7561 cgcgatcgcc gcgatgccgg tcagccgatt ccgctgtccc cccggaccag caccgttatg
     7621 ttccggactc gactcctgaa atgcattgta gaggctggaa cctggtgcag cctgggcacc
     7681 cggcggatcc agcggtctcc tgctttcttg aactcctcga acatctgata caggtactga
     7741 tcccgcttcc ggtgatgcca ccacgcgagt cgagcaaggt tcatgtcctg cgggtaccgg
     7801 tccggccccg tcagttcgct cccggagcct tggccaaagc ggacgggccg ttgatcaacg
     7861 cgacgccttc atcgaggcgg tcggcatatg ctgcgagcgc cttgccgatc atggc
//
LOCUS       DASVOR010000989         2727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_506585,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000989 DASVOR010000000
VERSION     DASVOR010000989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506585"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF516_19125"
     CDS             complement(<1..275)
                     /locus_tag="VF516_19125"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8109855.1"
                     /translation="MSLWSRVQRRLGDLAGELVLDEYREYLDRAQQLIAAGDLAAATE
                     LLETVLAAKPEHGQALIALGDARLIGRDPARALAAFERALELRPGDPA"
     gene            complement(338..2143)
                     /locus_tag="VF516_19130"
     CDS             complement(338..2143)
                     /locus_tag="VF516_19130"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HEX8109856.1"
                     /translation="MIFLEHHKQAKREIVARLQQLAELADAVGMITLARDIRTTRIPK
                     LESERFHLVVLGEFNHGKSTFVNALLGSDILPTGITPTTASINHVVFAQHATARVVLT
                     SGESKYLDPQQLKEWVTVAGGHASEVAYVEVGYPSQLLENHVVLVDTPGVNDLNEQRA
                     EVTYGYVPRADAVVFLLDAGQALKDSEREFLRSRVLESARDRLIFVLGKMDMLSSDER
                     IAVVDYVQGGLAQMMPEPVVFPLSAKDWAKSKDPASGMPELLAYLERFLARDRAQVIL
                     DNAAADAARTAGYLENNLGVRMRAYDLDLGELEQRIAAVRDQLDTSKRKLDELHVRID
                     ADAASIKNQVGLDLEAFARAFVAALPGQIDAVDANDVKAYLPAFIESKFKEWAELEGA
                     RLAAMLEHLAEQVIAITNENVATAAAALAERLGPEDTAVEISVDSFKYDIGIYAMGAL
                     GTTVMLFVNALAGGLLTIAAPILAIILKSKIAGDIRIQAKERVPGAVLKAAEAMKPHF
                     DKCIDDFAKRLSEFVSNAGNTLYKGISEILDRTMAERREHAGGLAELKSATTRQIEQI
                     RAAGSALRQLREGLWASDELGSSAAAAGALPPDVS"
     gene            complement(2181..>2727)
                     /locus_tag="VF516_19135"
     CDS             complement(2181..>2727)
                     /locus_tag="VF516_19135"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8109857.1"
                     /translation="TEDAPHLIVNLQLAERLDDTQLIAAIGHELGHVQNGHIFYATAL
                     HYLRHSAAFYVRWVVQPAIMTLQAWSRRAEVTCDRAALLAVRDLDKTLQALVAIELGL
                     DRGAAFSPEDYLKAVPDAKRGIGRYAELFRSHPQLPKRIQALRLFAGSAMYAQATGGD
                     PTGKPSLADVDKQVADLISVL"
BASE COUNT          364 a          924 c         1010 g          429 t
ORIGIN      
        1 gccggatcgc cgggccggag ctcgagcgcg cgctcgaacg cggccagcgc ccgcgccgga
       61 tcgcgcccga tcagccgggc gtcgccgagc gcgatcagcg cctggccgtg ctcgggcttg
      121 gcggcgagca cggtctccag cagctcggtg gcggcggcga ggtcaccggc ggcgatcagc
      181 tgctgggcgc gatcgaggta ctcgcggtac tcgtcgagca ccagctcgcc ggccaggtct
      241 cccagccggc gctgcacacg cgaccacagc gacatcgctc acagcttact ccaccggcca
      301 tggcaccgcc cggtgcggcg cccgggctcc ggagctgcta cgacacgtcc ggcggcagcg
      361 cgccggccgc cgcggcgctg gacccgagct cgtcgctcgc ccacagcccc tcgcggagct
      421 ggcgcagcgc gctgcccgcg gcgcggatct gctcgatctg gcgggtggtc gccgacttga
      481 gctcggccag gccgccggca tgctcgcggc gctcggccat cgtgcggtcg aggatctcgc
      541 tgatgccctt gtagagcgtg ttgccggcgt tggacacgaa ctccgacagc cgcttcgcga
      601 agtcgtcgat gcacttgtcg aagtgcggct tcatcgcctc ggcggccttg agcaccgcgc
      661 ccgggacgcg ctccttggcc tggatccgga tgtcgcccgc gatcttcgac ttgaggatga
      721 tcgcgaggat cggcgcggcg atcgtgagca gcccgccggc gagcgcgttg acgaacagca
      781 tcacggtggt gccgagcgcg cccatcgcgt agatcccgat gtcgtacttg aagctgtcga
      841 ccgagatctc gaccgcggtg tcctcgggac cgagccgctc ggccagcgca gccgccgcgg
      901 tggcgacgtt ctcgttggtg atcgcgatga cctgctcggc gaggtgctcg agcatcgcgg
      961 cgagccgcgc gccctcgagc tcggcccact ccttgaactt gctctcgatg aacgccggca
     1021 ggtaggcctt gacgtcgttg gcgtcgaccg cgtcgatctg gccgggcagc gcggcgacga
     1081 acgcgcgggc gaacgcctcg aggtcgagcc cgacctggtt cttgatcgag gccgcgtcgg
     1141 cgtcgatccg gacgtggagc tcgtcgagct tgcgcttcga ggtgtcgagc tggtcgcgca
     1201 cggcggcgat ccgctgctcg agctcgccga ggtcgaggtc gtaggcccgc atccgcacgc
     1261 cgaggttgtt ctcgaggtag cccgcggtgc gcgccgcgtc ggccgccgcg ttgtcgagga
     1321 tgacctgggc gcggtcgcgg gcgaggaagc gctcgaggta ggccagcagc tcgggcatcc
     1381 cgctcgccgg gtccttggac ttcgcccagt ccttggccga cagcgggaac accaccggct
     1441 cgggcatcat ctgggccagc ccgccctgga cgtagtcgac cacggcgatc cgctcgtcgg
     1501 acgacagcat gtccatcttg ccgagcacga agatcagccg gtcgcgcgcg ctctccagca
     1561 cgcggctgcg caggaactcg cgctcgctgt ccttgagcgc ctggccggcg tcgagcagga
     1621 acaccaccgc gtcggcccgc ggcacgtagc cgtaggtgac ctcggcgcgc tgctcgttga
     1681 ggtcgttgac gcccggggtg tcgaccagca cgacgtggtt ctcgagcagc tggctcgggt
     1741 agccgacctc gacgtaggcg acctcgctgg cgtggccgcc cgcgacggtg acccactcct
     1801 tgagctgctg cgggtcgagg tacttgctct cgccgctggt cagcacgacg cgcgcggtgg
     1861 cgtgctgcgc gaacacgacg tggttgatcg aggcggtggt cggcgtgatc ccggtcggca
     1921 ggatgtcgct gccgagcagc gcgttgacga acgtcgactt gccgtggttg aactcgccga
     1981 gcacgacgag gtggaaccgc tcggactcca gcttggggat ccgcgtggtg cggatgtcgc
     2041 gggcgagcgt gatcatgccg accgcgtcgg cgagctcggc gagctgctgg aggcgcgcga
     2101 cgatctcgcg cttggcttgc ttgtggtgtt cgaggaagat catggcgcct cggagggagg
     2161 gaagcgagag gaggatcgac tcacagcacg ctgatcaggt cggcgacctg cttgtcgacg
     2221 tcggccaggc tcggcttgcc ggtcggatcg ccgccggtcg cctgggcgta catcgccgag
     2281 ccggcgaaca gccgcagcgc ctggatccgc ttggggagct gggggtgcga gcggaacagc
     2341 tcggcgtagc gcccgatgcc gcgcttcgcg tcgggcacgg ccttgaggta gtcctcgggg
     2401 ctgaacgccg cgccccgatc cagcccgagc tcgatcgcga ccagcgcctg cagggtcttg
     2461 tcgagatcgc gcaccgcgag cagcgcggcg cggtcgcatg tgacctcggc gcggcgcgac
     2521 cacgcctgca gcgtcatgat cgccggctgc accacccagc ggacgtagaa cgccgcggag
     2581 tggcggaggt agtgcagcgc ggtggcgtag aagatgtggc cgttctggac gtggccgagc
     2641 tcgtggccga tcgcggcgat cagctgggtg tcgtcgagcc gctcggcgag ctgcaggttg
     2701 acgatcaggt gcggcgcgtc ctcggtg
//
LOCUS       DASVOR010000990         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_126778,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000990 DASVOR010000000
VERSION     DASVOR010000990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126778"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF516_19140"
     CDS             complement(<1..186)
                     /locus_tag="VF516_19140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109858.1"
                     /translation="MVHVEVDDGLNVHGHVNLNDGRQGQGSGRRQPGDAPEVGAATQR
                     QRGATRSSSTARPPPATA"
     gene            complement(351..>2034)
                     /locus_tag="VF516_19145"
     CDS             complement(351..>2034)
                     /locus_tag="VF516_19145"
                     /inference="COORDINATES: protein motif:HMM:NF023280.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-1,6-glucosidase domain-containing protein"
                     /protein_id="HEX8109859.1"
                     /translation="ATVPELGCVTPVIPNAGPDSEAQQAAVAAVRDQDCFNWGYDPWH
                     YTSPEGAFSTQPDDGASRIREMRAAVMALHEAGLRVGMDVVYNHTTASGQSDRSVLDR
                     VVPGYYHRLDATGNVLHDSCCEDTAAENAMMAKLMTESMVTWARQYQIDSFRFDLMSF
                     HPRAVIQELQARVDAATGRRVFMLGEGWNFGAVGNNARFVQASQLSLNGSGLGTFSDR
                     ARDFIRGGGPFDSGQSLVGNQGFVSGLFYDDNGSGAGKTRGDLMHAGDMVKVGLAGSI
                     RDYPLTTADDQTLQLQQIDYFGQPAGYVTDPQEVVNYFENHDNQTMFDISVYKLPVST
                     SREDRVRTQMLAAALDAFSQGVSYFHAGVETLRSKSMDQNSFNSGDWFNRLDWTFTDN
                     NFGVGAPLQSDNGGNWPILKPLLANPSIKPTPADLRLARDMFRDLLEIRASSPLFHLR
                     TAADIKQRLTFYNTGSRQEPTVVVGHLDGRGMRDAEDDLIYLVNVDKVAHTLTIDAEK
                     HKPYHLHPVQARFDAADHRPALQAGYDRATGAFTVPPRTSVVFVAGDRDRDD"
BASE COUNT          285 a          658 c          738 g          353 t
ORIGIN      
        1 cgcggtcgcc ggcggcggcc gcgccgtgct gctcgagcgg gtcgcgccgc gctgacgctg
       61 cgtcgcggca cctacctcag gcgcatcgcc cggttgacgt cgacctgagc cttgaccctg
      121 acggccgtcg ttgaggttga cgtggccgtg gacgttgagc ccatcgtcga cctcgacatg
      181 gaccatcggt cgcgattctt cgacgaggac tcggaggctg ccaaaggtct acgtacaagg
      241 tcgagggtgg agtcgacgtc caacgtcgcc gttcacgtca acgttttgcg acaacgtcaa
      301 ggtcgacctc aacgtctttg ccaaggacgt cccagccttt catcacatcg tcagtcgtcg
      361 cgatcgcggt cgcccgcgac gaacaccacc gaggtccgcg gcggaacggt gaacgcgccg
      421 gtcgcgcggt cgtagccggc ctgcagcgcc ggccggtggt cggccgcgtc gaaccgcgcc
      481 tgcaccgggt gcaggtgata cggcttgtgc ttctcggcgt cgatcgtcag cgtgtgcgcg
      541 accttgtcga cgttgaccag gtagatcagg tcgtcctcgg cgtcgcgcat gccccggccg
      601 tcgaggtggc cgaccaccac ggtcggctcc tggcggctgc cggtgttgta gaacgtcagc
      661 cgctgcttga tgtcggcggc ggtgcgcagg tggaacaggg gagagctggc gcggatctcg
      721 agcaggtcgc ggaacatgtc gcgggcgagc cggaggtcgg cgggggtcgg cttgatgctc
      781 gggttggcga gcaaaggctt gaggatcggc cagttgccgc cgttgtcgct ctgcagcggc
      841 gcgcccacgc cgaagttgtt gtcggtgaac gtccagtcga gccggttgaa ccagtcaccg
      901 ctgttgaagc tgttctgatc catcgacttg ctgcgcaggg tctcgacgcc ggcgtggaag
      961 tacgacacgc cctggctgaa cgcgtcgagc gccgcggcga gcatctgggt ccgcacccgg
     1021 tcctcgcggc tggtcgacac cggcagcttg tagacgctga tgtcgaacat cgtctggttg
     1081 tcgtggttct cgaagtagtt gacgacctcc tgcggatcgg tgacgtagcc cgccggttgg
     1141 ccgaagtagt cgatctgctg gagctgcagg gtctggtcgt cggcggtggt gagcgggtag
     1201 tcgcggatcg agccggcgag cccgaccttg accatgtcgc cggcgtgcat caggtcgccg
     1261 cgggtcttgc cggcgccgct gccgttgtcg tcgtagaaca ggccgctgac gaagccctgg
     1321 tttccgacca ggctctggcc gctgtcgaac ggcccgccgc cgcggatgaa gtcgcgcgcg
     1381 cggtcgctga acgtgccgag gcccgagccg ttgagcgaca gctgcgacgc ctggacgaac
     1441 cgcgcgttgt tgccgaccgc gccgaagttc cagccctcgc cgagcatgaa cacgcggcgg
     1501 ccggtcgcgg cgtcgacccg ggcctggagc tcctggatca ccgcgcgggg atggaagctc
     1561 atcaggtcga accggaacga gtcgatctgg tactggcgcg cccaggtgac catggactcg
     1621 gtcatcagct tggccatcat cgcgttctcg gccgcggtgt cctcgcagca gctgtcgtgc
     1681 agcacgttgc cggtggcgtc gagccggtgg tagtagccgg gcaccacgcg atcgagcacc
     1741 gagcggtcgg actggcccga cgcggtggtg tggttgtaga ccacgtccat gccgacccgc
     1801 aggccggcct cgtgcagcgc catcaccgcc gcgcgcatct cgcggatccg gctggcgccg
     1861 tcgtcgggct gggtcgagaa cgcgccctcg ggcgacgtgt agtgccacgg gtcgtagccc
     1921 cagttgaagc agtcctgatc gcgcaccgcg gcgaccgcgg cctgctgggc ctcggagtcc
     1981 gggccggcgt tcgggatcac gggcgtgacg cagccgagct cgggcacggt ggcg
//
LOCUS       DASVOR010000991         4812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_418498,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000991 DASVOR010000000
VERSION     DASVOR010000991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418498"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..809
                     /locus_tag="VF516_19150"
     CDS             <1..809
                     /locus_tag="VF516_19150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109860.1"
                     /translation="ILARRLVEEHEFLREDGRVRVILSVGDEGELRLAGASIPKSDPK
                     AFTSLTVVYDLLQYLGWDCPGAMRRSHQRPSDDVLDDSYKILVSRLDDLMKYCGDTRA
                     RLAGAGSAKEIRAPKDHEGAGHPFLRPVVQKAVARVASEIVQAGLLTWEDAMLRLAGL
                     EWKLSENPWRAVYAADAGKMLTTKEHTDLLCELLHVHLAPTSLAAIKRARKNFKDLRS
                     IQYEITEEELAERIVTAPQVPVSEIKLPEKEISEGLEITLPVDAESAGSE"
     gene            complement(923..1963)
                     /locus_tag="VF516_19155"
     CDS             complement(923..1963)
                     /locus_tag="VF516_19155"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8109861.1"
                     /translation="MPVAMTLVNAMTPRPHRSRRLGIALDLVSAAFDTTRPLITNLII
                     TRRCNLSCGYCFEYDKVSPPIPLATLRERIDHLARLRAVFVTLTGGESLLHPDADALV
                     AYVRERGMTPFLNTNGFLLTVDWIERLNRAGLYAMQISVDNVHPNATSKKSLKTLLPK
                     LQLLAEHARFRVRINCVLGSSPPAEAVEVARVALGLGFDVSTSLIRHGDGSARPLDTE
                     TRAAYEQIRSLGSRAPRYLSDDFTLRLIEHGTADWKCRAGARTFHVCEDGLVHLCAPR
                     VGSPGIPLADYTAEHIRRAFDTPKPCAASCPIAYAHHASRLDRFRGQRSAPLALPPPE
                     PPLVQLRPRRAA"
     gene            complement(2074..4389)
                     /locus_tag="VF516_19160"
     CDS             complement(2074..4389)
                     /locus_tag="VF516_19160"
                     /inference="COORDINATES: protein motif:HMM:NF020471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEX8109862.1"
                     /translation="MSTCTHCGQRKGKRSCPALAGAICSRCCGQHRLVEIACPSDCVH
                     LGGLAVVRDPSRAVPFTRADHLSAWDKLQAFAVSATGFDREAAARLGGGTRWDPSIAL
                     AYVHYGHRGVDGSRMIERFLAARGRTLSAGEAAATVALQRARASLFEVVAVRTGVGLD
                     LRDALSGETSHVREVTGSARLREHDVLFAWVMDFPDHRELTGAACDIERAHVERVRAA
                     LEAELPAARTRWPGIADADLVGSIAWAVFRALDAARDEEDARREAATEPPPPTFDDDD
                     DDRDEDDDPGTGWSYDASTAPDPERWLTADELQKIAAIEAHHRALGVDLPNPRMHATM
                     HAIVENQLAAGAPAEARATLERFMAAGVARHDAVHAIGSVVAKAIWRMLRRHETVDRD
                     AMVAALARLDPEDWRDGEPEDEPPATIPPLAHEVMYRRVPGLRAAIEHAAHGMRQGGW
                     TESTLLDPPQLLERSTIGEFLELHAAALMAAGWAREAAIDDADLLSIHAFNILTYELH
                     RKKTFWVDESLAFMLGHTRLDVRGEGFRLPFPCFALVFTDRETLGLAEAVARTDPTAS
                     VCGLPLRGLTVYATQLPTTRGALGLHLAFLLDADTDSWPWLITRDLDIQPDDTLDDII
                     ESRFADAVNPDPVFRTPELRQLVQLVVNAILFAGSSPAWPVVGSAASPAAAEPAGRPR
                     RRRAPPPPPERSEEQIWHLPGKIPIRQVRALRQLQRDRDRGALFSRFMVRGHWRRAPG
                     TWRDQAPRWIAPYWKGPPLGDIVERDYALKP"
BASE COUNT          784 a         1476 c         1802 g          750 t
ORIGIN      
        1 ctatcctggc gcggcggcta gtggaggaac acgagtttct tcgtgaggat ggccgtgttc
       61 gcgttattct gagtgtcgga gatgaaggag aactccggct agcgggtgcg agtatcccga
      121 agagtgatcc taaagcgttt acctcactga ccgtggtcta tgacctgctc cagtatctcg
      181 gatgggattg tccaggagcg atgcggagga gtcaccagcg accaagtgac gatgtccttg
      241 atgatagtta taagatcctg gtaagccggc tagacgatct gatgaagtat tgcggtgata
      301 cccgagcgcg actagcagga gcgggctccg cgaaagagat tcgtgctccc aaagatcacg
      361 aaggagccgg acatccattt ctccgacctg ttgtccaaaa agccgtggca cgcgtcgctt
      421 ctgagatcgt tcaggccggc ctattgacct gggaagacgc aatgcttcgt cttgctgggc
      481 tggagtggaa gctctctgag aatccgtggc gtgcagtcta tgccgctgat gctggcaaga
      541 tgctgacgac caaagaacat acagatttgt tgtgcgagct tctccatgtt catttggcac
      601 cgacatcgtt ggcagccatt aagagagcac ggaaaaactt caaagattta cgcagcattc
      661 agtacgagat taccgaagaa gagcttgctg aacgcatcgt tactgcacca caagtaccag
      721 tttcggagat caaattgcct gaaaaggaga tatccgaggg acttgagatc accttgcccg
      781 tagatgcaga atcggctgga tcggaatagc aattgcatac gcgagagtgc agtgcctgcg
      841 gaagcgcatc tggcatggcg aaacggccgc atgactgctt acgtgcttcg cgatccccga
      901 tgttcgtgcg ggtaggttgc catcaggcgg cccggcgcgg gcggagctgg accagcggcg
      961 gctcgggcgg tggcagggcg aggggcgccg agcgctggcc gcggaagcgg tcgaggcgcg
     1021 aggcgtggtg ggcgtaggcg atcgggcagc tcgcggcgca cggcttgggg gtgtcgaagg
     1081 cgcggcggat gtgctcggcg gtgtagtcgg cgagcgggat gccggggctg ccgacgcgcg
     1141 gggcgcagag gtggaccagg ccgtcctcgc agacgtggaa ggtgcgggcg ccggcccggc
     1201 acttccagtc ggcggtgccg tgctcgatca ggcgcagggt gaagtcgtcg gacaggtagc
     1261 gcggggcgcg cgagccgagg gagcggatct gctcgtaggc ggcgcgggtc tcggtgtcga
     1321 gcggcctggc cgagccgtcg ccgtggcgga tcagcgaggt ggagacgtcg aagcccaggc
     1381 cgagggcgac gcgggcgacc tcgacggcct cggcgggcgg gctcgagccg aggacgcagt
     1441 tgatgcggac gcggaaccgg gcgtgctcgg cgaggagctg gagcttggga agcagggtct
     1501 tgagcgactt cttggaggtc gcgttggggt ggacgttgtc gacgctgatc tgcatggcgt
     1561 agaggccggc gcggttgagg cgctcgatcc agtcgacggt gagcaggaag ccgttggtgt
     1621 tgaggaacgg ggtcatgccg cgctcgcgga cgtaggcgac gagggcgtcg gcgtcggggt
     1681 ggagcagcga ctcgccgccg gtcagggtga cgaacacggc gcgcagccgc gcgaggtgat
     1741 cgatccgctc gcgcagggtg gcgagcggga tcggcgggga gaccttgtcg tactcgaagc
     1801 agtagccgca ggagaggttg cagcgccgcg tgatgatcag gttggtgatc agggggcgcg
     1861 tggtgtcgaa cgcggcggac acgaggtcga gggcgatgcc gaggcggcgg ctgcgatgcg
     1921 ggcgaggggt catggcgttg accagggtca tggcaaccgg catgccgcgg ctaacttcgc
     1981 ggtgcgtcag ggcctgcgca ccggcggccg ttcgaacctg tcaggttgcc aacgcgcgtg
     2041 atggtcttcg cgcaccttgc cgggacggcc tgatcacggc ttcagcgcgt agtcgcgctc
     2101 gacgatatcg ccgagcgggg ggcccttcca gtacggcgcg atccagcgcg gagcctggtc
     2161 gcgccaggtg ccgggcgcgc ggcgccagtg gccgcggacc atgaagcggg agaacagcgc
     2221 accgcgatct cgatcgcgct ggagctgccg cagggcacgg acctgccgga tcgggatctt
     2281 gccgggcagg tgccagatct gctcctcgct gcgctccggc gggggcggcg gagcacgcct
     2341 tcgccgggga cggccggccg gctcggcggc agcgggcgaa gcagccgagc ccacgaccgg
     2401 ccacgccggg gacgatccgg cgaacaggat ggcgttgacg acgagctgga cgagctggcg
     2461 cagctcgggg gtgcggaaga ccggatccgg gttcaccgcg tcggcgaacc ggctctcgat
     2521 gatgtcgtcg agcgtgtcgt cgggctggat gtcgagatcg cgcgtgatca gccacggcca
     2581 cgaatcggtg tcggcgtcga gcaggaacgc gaggtgaagc ccgagcgcgc cgcgcgtggt
     2641 gggcagctgg gtcgcgtaga cggtgaggcc gcgcagcggc aggccgcaga ccgaggcggt
     2701 cggatcggtc cttgccacgg cctcggcgag tccgagggtc tcgcgatcgg tgaacaccag
     2761 cgcgaagcac ggaaacggca gccggaagcc ctcgccacgc acgtcgagcc gcgtgtggcc
     2821 gagcatgaac gcgagggact cgtcgaccca gaacgtcttc ttgcggtgca gctcgtaggt
     2881 caggatgttg aatgcgtgga tcgagagcag gtcggcgtcg tcgatcgccg cctcgcgggc
     2941 ccagccggcc gccatgagtg cagcggcgtg cagctcgagg aactcgccga tcgtcgatcg
     3001 ctcgagcagc tgcggcggat cgaggagggt cgattcggtc cagccgccct ggcgcatgcc
     3061 atgggccgcg tgctcgatcg ccgctcgcaa gcccggtacc cgccggtaca tgacctcgtg
     3121 tgcgagcggc gggatcgtcg ccggtggttc gtcctcgggc tcgccgtcgc gccagtcttc
     3181 ggggtcgagg cgggcgagcg cggcgaccat cgcgtcacgg tccacggtct cgtgccgtcg
     3241 cagcatgcgc cagatcgcct tcgccaccac ggagccgatc gcgtggacgg cgtcatgtcg
     3301 tgcgacgccg gcggccatga agcgctcgag cgtcgcgcgg gcctccgccg gcgcgcccgc
     3361 cgcgagctgg ttctcgacga tggcgtgcat cgtcgcgtgc atcctggggt tcggcaggtc
     3421 gacgccgagc gcacggtgat gcgcttcgat cgcggcgatc ttctgcaact cgtcggctgt
     3481 gagccagcgc tcgggatcag gcgcggtgct cgcgtcgtag ctccagccgg tgccgggatc
     3541 gtcgtcctcg tcgcgatcgt catcgtcgtc gtcgaaggtg ggaggcggcg gttcggtcgc
     3601 ggcctctcgg cgcgcgtcct cctcgtcgcg cgctgcatcg agggcgcgga acacggccca
     3661 ggcgatcgat ccgacgaggt cagcgtcggc gatcccgggc cagcgcgtgc gcgcggccgg
     3721 tagctcggct tcgagggcag cgcgcacccg ctcgacgtga gcgcgctcga tgtcgcacgc
     3781 cgcgccggtg agctcgcgat gatcggggaa gtccatcacc cacgcaaaca ggacgtcgtg
     3841 ctcacgcagc cgcgcagacc cggtcacctc gcgcacgtgc gacgtttcgc ccgaaagcgc
     3901 atcgcgaaga tcgaggccga ctcccgtgcg cacggccacg acctcgaaca gcgatgcccg
     3961 cgcgcgctgg agcgcgacgg tggcggccgc ctcgcccgcc gacagcgtac gtccgcgtgc
     4021 cgcgaggaag cgctcgatca tgcggctgcc atcgacgcct cggtggccgt aatgaacgta
     4081 cgcgagcgcg atgctcggat cccaccgcgt cccgccgccg agccgcgcgg cggcctcgcg
     4141 atcaaagccc gtcgcgctga ccgcgaacgc ttgcagcttg tcccacgcgg agaggtggtc
     4201 cgcgcgggtg aacggaacgg cgcgggaggg atcgcggacg accgccagcc cgccgagatg
     4261 gacgcagtcg gacgggcaag cgatctcgac gagtcggtgc tggccacagc agcgcgagca
     4321 gatcgcaccg gcgagcgccg gacagctgcg cttgcctttg cgctgaccac agtgagtgca
     4381 cgtcgacacc gagcgtacgt accgcaggtc gatccccggt gccaggtccc atgccagctc
     4441 accgtgcgaa cggctcggtg accgcagaac tggtggacgg tggcgagcct tcgcgacacg
     4501 aagcagcgtc ggcgctgccg gatgcggggg aacggttcga ttccatcgag gatcgttgtg
     4561 cgcaacgtca tcgttccccg cgacgtcaga cccggcggct accgtcggat cgtgcccagc
     4621 cggaacggcc cctgcccctg cggttcgacc ctcaagtaca agaagtgctg cctcgcgaag
     4681 gatgaagcag cacagcgcgc gctcgcgccg ccaccacggc cagcgccgcg gatcctccat
     4741 caccgcggtc gatcgttgcc ggtctccgga gatggcgttt ggccggcggg tgtcctcgac
     4801 cgcgccgacg ag
//
LOCUS       DASVOR010000992        28559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_477656,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000992 DASVOR010000000
VERSION     DASVOR010000992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 28559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28559
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477656"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /gene="rsmI"
                     /locus_tag="VF516_19165"
     CDS             complement(<1..618)
                     /gene="rsmI"
                     /locus_tag="VF516_19165"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEX8109863.1"
                     /translation="MIGTLFIVGTPIGNLEDLSLRAARVLREADLIAAEDTRAARHLL
                     AHVDALGPSNPARRIVSNFEGNEAERADEVARALQGGQRVAVISEAGMPGVSDPGAYA
                     IAAAIAAGARVEVVPGPSAALAALIGSGLPSDRFTFVGFPPREPGARRALFGTLRGES
                     ATMIFYEAPDRLAATLQDAAAAFGADRRASLGRELTKLHEEHVRGT"
     gene            901..1785
                     /locus_tag="VF516_19170"
     CDS             901..1785
                     /locus_tag="VF516_19170"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8109864.1"
                     /translation="MSVGTTGSGVRTFRGVHVAITGASSGIGEALARELGAAGARLTL
                     VARREERLHALAAELAAGERSGGSFVAAVDLADMERATSWIQPAEAALGPIDVLVNNA
                     GVQQVAAAADLDAGECEYVLALNVRTPMRLICELLPSMLSRRRGTIVDVASMAAITPM
                     PGMFYYNAAKAALANASEGLRGELRGTGVHVVTVYAGPVDTEMSRIGASRLEDTLAKS
                     LAPMGKPAELARLIRQAIERREPRVIYPRVYSVGTWFPSVSRWCTARWSPAPKRRAGS
                     SPVQQSSHMRRGERRGER"
     gene            1787..2941
                     /locus_tag="VF516_19175"
     CDS             1787..2941
                     /locus_tag="VF516_19175"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8109865.1"
                     /translation="MAAGARQQQRRLGLVFSGAGARAVFQVGAYEVLVQDPRFAGAEF
                     LSCTSGGSINAAMVASGKTPAQMMDFWLRFADTPPVHANKAFFREAVVTLAKVSLPFS
                     SRTRTGATLGWLFGRLLHHLSPDPGNLLALGLEILLTRRWDLVDRFLTQMTESSLLHS
                     GPLRDKIVQGIGGETLPTGRIHIAINTIDVACSEPVRIVTQEAARGLRLNDRYLVVPA
                     ITVDHVVASAALPLLLPPVAYAGRYLIDGGLLVNSALAPAVDLGADIIVPILAMAKAD
                     ARLSQYKNLNEAVERIAESLFESHYQSDRKLLLDRNRLSGSAPGSQTRYREVKLYQAI
                     RPPDSSMFDVGSYLYFSRGLLGEMYEAGKAAAAAWLKQGPLLDSLRPESP"
     gene            3111..3485
                     /locus_tag="VF516_19180"
     CDS             3111..3485
                     /locus_tag="VF516_19180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109866.1"
                     /translation="MYLAAPWSAFVALQAIRPFAGLIGRTAVQRFLTRQLRRAPIGPS
                     EVSRRSARTWYWAAAENAKGQRIAAQIDLPDVYDLTTSAALAAMHQVLGGHVAPGAWT
                     PAKAFGAGFVTRLPDTHLRMLS"
     gene            3523..4395
                     /locus_tag="VF516_19185"
     CDS             3523..4395
                     /locus_tag="VF516_19185"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEX8109867.1"
                     /translation="MKHYDIDSLENRDPRAIARIFAVAEPIFRWYFRPDIQGLENIPR
                     GAGLYVGNHNSGALAPDMFIFAMAVHRVRGIEHMPYALAHEVTIRIPLIHQLIVPLGA
                     VRASHDAAHRVLAAGSKVLVYPGGDIDAFRPFRDRNRVVFGARRGYIKLALRENVPII
                     PVVAEGAHSTLIVLHDGRWLARTLRIDRLFRYQVFPVTLSVPWGLTIGMPLPHFPLPV
                     RIRIRVLEPIYLDRHGEAAADDRDYVEHCHCLVHSRMESTLQSLAQDRRAAGSPRREP
                     QRSGSIGERPGFSG"
     gene            4500..4799
                     /locus_tag="VF516_19190"
     CDS             4500..4799
                     /locus_tag="VF516_19190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109868.1"
                     /translation="MVAVNTSVDEIVAFVKLYLLSEFLPGKTGVAIDSSTRLNKSGVL
                     DSLKLVRLVTFLEESYRIEIQPHEMAPKHFETLQAIAALVHSKLVMPAEPAPSIE"
     gene            4796..5572
                     /locus_tag="VF516_19195"
     CDS             4796..5572
                     /locus_tag="VF516_19195"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8109869.1"
                     /translation="MTMGNYYLVCAAVLVATYTVNTTYVTVFYHRGLAHGGVELRPGV
                     RRFVAATGSWVTGLDPKAWACMHRQHHQYADTARDPHSPRNVGILGVLVAQFIAYKAN
                     LALLIMGHPKYTKIVEDIEFPVSWINRRSWVIPYAVHLAIAIAIGAAAGAWLLGAAYF
                     IGLMSHPIQGWMVNALGHAHGYRNFDTPDGSRNNTLVALLVVGEGYQNNHHRNPCSPT
                     MAVKWYEIDLGYVLCRILGLCGLVTIPISPSTHQPSGSAP"
     gene            5569..6225
                     /locus_tag="VF516_19200"
     CDS             5569..6225
                     /locus_tag="VF516_19200"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8109870.1"
                     /translation="MTEVADFVRGVDTLVELLQHRAAERPDRTVFTFLADGELEGVRW
                     SYAELDRRARAIGAVLQDAGAAGQRAILIYPPGLEFLSAFFGCLYGGVVAVPAYPPDP
                     GRLARTLPRLCAIARDAETGFVLTTNDILRLASDVTNAEPDLARLRWIASDAVPAGSE
                     SGWRPPGVGRDSVAFLQYTSGSTAAPKGVIVRYRNVMHNQHMIQRQACRAAWLEGTLQ
                     "
     gene            complement(6232..11571)
                     /locus_tag="VF516_19205"
     CDS             complement(6232..11571)
                     /locus_tag="VF516_19205"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX8109871.1"
                     /translation="MSEETRRSVGTALSPAKRALLERWSRGHDAPAAPAIPRRPPGAV
                     PASFPQQRIYFMEQLEGGTSANNLIYAARLDGRLDIGALERSCAAIVERHEVLRTTFA
                     MPAELPVQVLRPVDATALPVVDLTELAPDARIAEARRHALAEAQRPFDLQSGPLRRAT
                     LIRLSHSEHWFVLVTHHSVFDGWSMGVFVQELGSFYQAFAGGGTATLPPLPIQYADFT
                     LWQSQRLDSPELGAQRRYWLARLGSRLPVLELPGDRPRPPHQTYRGDTRSAVLPESVS
                     AALKALSSREGCTLFMAMLAAVKALLFRYTGQTDMLIGTPIANRNHPDIEGLIGMFVN
                     ILVLRTELAGDLSFRTLLRRVQASAVEAYANQDVPFEVVVKDLQPERALSRSPVFQVM
                     YVLQNSPLPPLALPGLTLTQLDLHSGTSKYDLTWFTTDTEHGLVNTIEYNTDLFDAAT
                     ITRMLQHFTAMLHGLIADPDLPIARLPLLTEPERRQILSTWNATHAELRDGGVHELFE
                     ASAAHAPDAVALVHAGRRMTYGELDRKAGRVASRLAAEGVGCGALLALHAERSPETII
                     CLLGILKVGAAYLPLDPRLPAERVRFMLEDSRACLLVTCSQLVGDLVAGAGMRVLHVD
                     AGLLDGPADEPPRARTAGSGTDIAYVMYTSGSTGQPKGVAIAHRAVVNLITAMQRALE
                     FGSHEVFLSVATLAFDISVVELFLPLSMGATVVLAEDLDVTDGSRLAVVLDRCGATTM
                     QATPAGWRLLLAAGWRGKPGLRILCTGEALPRDMAEQLLTCGSAVWNLYGPTECTVWA
                     TWTRVRSGTGPVPIGRPVPNLRIYILDALKQPVPIGVPGRLHVGGVGVARGYLNRPEL
                     TEKCFIADPFLDRREDARLYDTGDVARYLADGTVEFLGRDDDQVKINGMRIELGEIEM
                     VLRQHPDIGAAAVLARSGHDGGHQLEAFVVPAAPAGERMAPEQNDLARRLREFAKRKL
                     PGWMVPVRFAVLDALPVTATGKIDRHALADRPPAAPARECYVAPRNPVEQRLAEIWRS
                     RLHVDKVGVHDNFFNLGGASLVSLQVVVDARDAGIYLSPAMVFQYQTIAELAAAIEAS
                     NEASTPHTDAAPEDLEPMARTRTVIESLGVYLPPREETTEQVLRDCKKPVRFPLEAFT
                     GIRSRRVVGDGEFAVTLARKAIADCLSRSGYRPDEVELLICCNISRYDGPEVRFTFEP
                     GTALRLKHELGLDRAIAFNLSNACTGMWTGILLVDAFLRRGLIRCGLVVSGEYITHIT
                     RTAQREIDGFMDPRLPCLTVGDAGAAVMLARSTAPGVDAGFHDIELYTLGRYSPYCIA
                     KATGGDSPGAIMHTDSVKLSSVALQGGISHSLATLERNDWSLDSIAHLVLHQTSNTTL
                     REAAREINRRAGREVITDAVLTLNLKERGNTATTTHFVALMDQIRSGQIKSGERIVFG
                     LSGSGLTLGTALYVLDDLPDRILQGAPGARARAVPPAPRSAPAAARRPGMCIESIGLL
                     PSTITPGRSAVDLGRHAAQNCLRESAVRRRDIDLLLYTGVYREDYLCEPAVASLLAGD
                     LGINDDVDGDDSRTTLAFDVLNGGLGTLNACHLAVELLRAGDRDNVMVVAAEIENNAD
                     DPGRGLRGIAETGSAFVLRESSDGSTGFIDFHFKDYPAYLNALASHTHCNGSAIYLQV
                     QADPDLESAYLRCILDAVHETLAIAHLPASEVRWMLPPQISSSFITRLADALHLPADR
                     MVDAVRDRRDLFTSSLAFAMREVRDSGRVRPGDLGLIIGVGSGIQVGCALYRF"
     gene            complement(11568..17384)
                     /locus_tag="VF516_19210"
     CDS             complement(11568..17384)
                     /locus_tag="VF516_19210"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8109872.1"
                     /translation="MLTKLTLQTSGAQMKVHDIQAWLTTRLSRALSIDADQIDPRAPF
                     ALYGVTSMMAIDISRELETWFGRRLSPTLAYDYPCIAALAAHLGDNEAPEPAAEVQPQ
                     PVASGPIAIVGISCRFPGARGPAAYWRLLCDGVDAIREVPEQRWPAADYYDRDAMAPG
                     KANTRWGGFLDQIDQFDPWFFGISPREAERMDPQQRLILELAWEALEDAGIVPRKLAG
                     TRTGVFVGASVNEYSGIQLSDLRLVDNFAGTGNALSILANRISYLFDLHGPSITVDTA
                     CSSSLVAVHHACKSLQSGESTLALACGVNLILSPAIAIGFAKAGVMAADGRCKAFDAA
                     ADGYVRSEGGGVVVLKPLAGALADGDRIYGIVRGSAVNHDGRTNGLLAPSRQAQEAVV
                     RAACRDAGISPADIQYVEAHGTGTSVGDVIEAAALHAVLSTDRPAGAICAIGSVKTNI
                     GHTEAAAGIAGMIKVALAVNHGVIPLQLHFRTPNPSIRFEDTCLRVQTSASPWPATGS
                     PLAGVSSFGFGGSNAHVIIEGPPAAAERRAEPARAYHLLPISARSPQALRELARAHAR
                     ALAELSSAPGALHDVCFSASVRRGHLEHRLAVVGRSSRDLAELLEGFAAGQLYPGVHA
                     GREAAGQARRLVFVFSGQGPQWWPLARDLLDEPVFRAELDRVELALRRHQPELRLLDH
                     IQHDEAAGVLVDPVLGQPTLFAVQVALAELWRSWGIEPDAVIGHSMGEVAASDIAGAL
                     ELDAAARVITERGRAIKRASGRGAMALVDLPLPRVRQALDAHPGQLFVAAHNGPESVV
                     ISGQPEALAELVSRLSLDGVTVRTLEAVDYASHSPFMEPVSEQLATALANLQPRPARL
                     TMVSTVTAEPIAGDALDASYWGRNLREPVRLFEAIEQLAAEATPVFVEIGPHPVLTRA
                     VAQTLAHLGVRGEAVASLQRGDDGVATLLGSLAALYAQGHAVRWDGVYPSGGVPAPLP
                     SYCWQRERFWLGDLVNGANARPARPSGSQPGEGGHPLLQHHTHLMHPEGVHLWEATID
                     RRIPSWVADHQVYGEVVFPGAGFFEMSLSALEQSGMARRYTASDVALLAALSLDGKEP
                     RHLQVTIAPSAGDHWSLNVFSSPVSKRSLRESWTHHMTATLVRDEIDRSLRPPAWASI
                     DEIEQRCSESIAPQAFYQRLWDRGLQLGATFRGVKQIWRREGEALARIEIPEISHDEL
                     AAYRIHPAVLDACLQVLSATQVASDALDMFVPVGCKRIQLFEPGPALWSHVMRHGALD
                     HSAIAFEVDVRLLDDSGRVIAELEGVRLQRIARGALQIDHRKRDTWLYETAWRTHRRL
                     DDVVEPAGAAEQSGDRWLVFADRGGVGLGLVSLLENRGYCCVTVFAGGARQVAANGRD
                     ITTPPGRELIHPLILEQIKSGDTRWRGIVYLWGLDAADPDHADALETSLDICDPVLGV
                     VSGLVESGAYPPPRLWLVTRGAQPVGAAAGSLAIAQSPLWGLANVIALEHPEQRCSRI
                     DLDPAAGPGDAVQLAGLLGSADAEDQIAVRDGTAHVPRVVPYVTGRSRRQPSSLRDIS
                     AVVRPDAIYAITGGLGGLGLVVAQWMASQGARQLVLIGRSPPSSTAENVIAALRRDGV
                     EVTTAQADVTDRDAMAELLRALSSQAHPLRGIVHAAGILDDGALVAFDRARMRRVMAP
                     KIAGAWNLHRLTRDAALDFFVLFSSAVSVLGSPGQGNYSAANVFLDALAHLRRAQGVP
                     ALSINWGPWAEVGLAAAAAGKVRARGLVAARFVRMLSPSSGTETLERLMREDIAQVMA
                     LPYDLCNMLQFYPLGTGSAMLAEIAGQEPGAVGGVEAGHRTRVRRSEQQVYVEPETAT
                     QRTIQGIWERVLAIDGIGTRDSFFELGGDSVLAGQILNQINRTFAVRIDPTACYRSFT
                     IETMAELVESELMQKLDALSEEDALRLLARSPQDRTGP"
     gene            complement(17452..18729)
                     /locus_tag="VF516_19215"
     CDS             complement(17452..18729)
                     /locus_tag="VF516_19215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8109873.1"
                     /translation="MKFGIFYAHQIPRPWSGDHERTFFNDALEQAEIADRIGIDYLWA
                     QEHHFLEEYSHSSAPEVFLAACSQRTRTIRLGHGVVLMPPAYNHPARVAERIATLDLI
                     SNGRVDWGTGESSSRLELEAFGIDYVDKRAMWMEAVREAVRMVGMEPYPGFDGQYFSM
                     PCRNIVPKPLQKPHPPLWLACSNRDTMKLAARLGMGALTFAFMDAREAKFWVEEYYEV
                     FRNHCTPIGIEPNPNVAMLAGFMCHEDEDIAQQRGLDGLRYFAYGLNHYRTGTHVPGR
                     LDMWEEFKKLPPFVMAGTGGISSPAKIQDYFSTFEAAGVDQLILLQQSGNYRHEHVCE
                     SLELFGTKVLPSFKDRHDALDRNKREALELYTDRARANVVPMEPMLEVPPVESHQALM
                     MQANVDHGRMSGNRSMNATALWKLHVVGGHPAK"
     gene            19883..21586
                     /locus_tag="VF516_19220"
     CDS             19883..21586
                     /locus_tag="VF516_19220"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEX8109874.1"
                     /translation="MDGDFSTWVDLVQFRARTQPKDVGYIFVRDGTTEAQSHTYEELQ
                     ARAVAIAAQLQATHAPGERALLLYPPGLEFIPAFLGCLYSGVLAVPAYPALRAQNLPR
                     LEAIAADSQATVALTTSGLLDGPARRSPALEGLDWIASDASPPSPGGSWTRPALDGDS
                     LAFLQYTSGSTGTPKGVMVSHGNLLHNQRMIEQAFDHDQDTVVVGWLPMFHDMGLIGI
                     VLQPLYLGRPCIVMAPTDFVQQPLRWLRAISHYRATTSGGPSFGYGLCERKISPSEAA
                     SVDLRSWRLAFNGSEPVRADVMDRFCERFASSGFQRKAFYPCYGLAEATLLVSGGSAS
                     RAPVVQTVRASALERRKFEPGVPGSMLVGCGQSWLGQTIEIVDPETRVRCPPGQIGEI
                     WVRGPSVTKGYWRRPRDTQETFGARLEQGRDAFLRSGDLGVVHDGELFVTGRVKDIII
                     IRGRNHYPQDIEATVQRSHVALRADCGAAVAVEIDGEERLVVIQEVEREHLGQLDTKA
                     LIADVREAVASQHDIFVHAIVLLKPGAIPRTSSGKVQRHACATAFMAGQLAVMAPEIA
                     P"
     gene            21664..22617
                     /locus_tag="VF516_19225"
     CDS             21664..22617
                     /locus_tag="VF516_19225"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8109875.1"
                     /translation="MSGIAKPGIRDTATDSRSDKVFAVIVTFLPPLSVALAVILLYYG
                     WYQLTSIEVGLMLGMHFLCLVGVELGFHRLFSHCSYRAHRQVRMTLAALGSMAFEGPV
                     IWWAATHRRHHRLSDKPGDPHSPHLHTGLWAGFRGFVHAHIGWFWIPSSMRPPGWARA
                     VTDLYRDPDIFKIHMHYFYWLAAGFALPAALGGILHGSWKGVLLGLLWGGPIRVFAMN
                     HLTYWCINSVTHSRFGSRPFRTTDRSSNVPLLALPTLGQSWHNNHHAFPSSSTTSIAW
                     WQVDTGDWILRVLNRLHLVWERKLPTEQQRARKARRDEQKP"
     gene            22614..22892
                     /locus_tag="VF516_19230"
     CDS             22614..22892
                     /locus_tag="VF516_19230"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8109876.1"
                     /translation="MTEAVQPVIHAEASLREKEIQDWLTLSISRLIHVDPSDLDVNVS
                     FDRYGLDSAAAVELTGDLERWLGRKLTPTLFYDYLTIRAVSAYLAVAG"
     gene            22899..23804
                     /locus_tag="VF516_19235"
     CDS             22899..23804
                     /locus_tag="VF516_19235"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8109877.1"
                     /translation="MTTPTSTRLDSPGASLEAIQDHYDVSNDFYRLWLDSNMVYSGAM
                     WEPGDTLETAQLRKIDYHIAQAKAATSKRVLDVGCGWGAVLKRLAHTHGVDHVLGLTL
                     SAAQAEWVRGLGIPQITVKVESWTDHVPGAPYDSIISIGAFEHFARLEFSEAQKVAAY
                     RNFFAWCQERMVPGGYLSLQTFAYGNTRSREEAMKMPATQFLANEIFKETDPPRLANI
                     AEATEGSFEIVSLRNDREDYARTCREWLARLQARREAAVAVVGEEVVARYERYLKYSF
                     IGFETGNLALFRITLRSLNTRWRKR"
     gene            23801..26083
                     /locus_tag="VF516_19240"
     CDS             23801..26083
                     /locus_tag="VF516_19240"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8109878.1"
                     /translation="MNDPGAGPAIAIVGWACRFPGASSPEALWQLLATGADPIGVVPP
                     NRWDADALYAPAPRTPGKMNTRWGGFLDDVDAFDAEFFGMQPREACAIDPQQRIALEV
                     AWSALEDAGIVPARLAGTSVGVFFGASNWDYNNILNRDIAVAEEHSGPGTALSVISNR
                     VSFALDLKGPSITVDTACSSALVALHWACHSLRTRESSLALVGGVNLILSPETSIVLS
                     QGRALSSVGRSRTFDEGADGFVRSEGCAVLVVKRLDQAEADGDRILGVVRGTAVNHNG
                     ASYGLAAPYGPAQEALLRQTLAQAGVVPNELSFVEAHGTGTALGDLIELRALRTVLLE
                     GRSPQQRCAIASIKSNIGHTEAASGLAGMIRVLLSMQHGVITPNLHLQKLSPHIATAI
                     DGTPLFIPTQAEPWTVAGRRLAAVSSFSFGGANAQVILEQARGPAAAPDRSSPPSPHA
                     LMLSARSTEGLRELARSYAAYLTAHPSTALVDVCFTANTARSQFDHQLCAVSTSVDRL
                     GRQLSDFAAGRPAEGLWAGQRSGRKRPTIVLQLRGAGPTPPHTSMRRLVERCAPLLPA
                     PLLCQVSLSAEQGEPLSPSAYRAVHVAVQLTVARQWMAWGLRPTAVTGDGVGEIAAAC
                     VRGSMTTEEAWRYVLSDGELPRPVPPAPFERAGKLMCLAISSDTETLSVEDPSGEPRV
                     LVRGELIDGERQTHFHCLANLAVHGADIDWRAVYSEFPAQRIALPTYPFQRQRYWMAA
                     GSAAPAPAGRDSGGRASNLL"
     gene            complement(26766..26921)
                     /locus_tag="VF516_19245"
     CDS             complement(26766..26921)
                     /locus_tag="VF516_19245"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8109879.1"
                     /translation="MIGTLFIVGTPIGNLEDLSLRAARVLREADLIAAEDTRAARHLL
                     AHGRSRP"
     gene            27136..27468
                     /locus_tag="VF516_19250"
     CDS             27136..27468
                     /locus_tag="VF516_19250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109880.1"
                     /translation="MDNDDRRERAMDGDDLTGHVLVAIRRDISDLRADMDERFERVDA
                     RMDRLGDRLDSPITETQMRLSTQIFELHGRVNDLQGTVHDMQGKLDDVHGMLRDLHGR
                     FGRRKPRN"
     gene            complement(27520..>28559)
                     /locus_tag="VF516_19255"
     CDS             complement(27520..>28559)
                     /locus_tag="VF516_19255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase factor sigma-32"
                     /protein_id="HEX8109881.1"
                     /translation="AHDADGEPETDGEAEIDAASGGADTDLATAAAAIEPDEVLEDDA
                     EAAPAESRGSSLAKRDPMAVYLRETRRYPLLTPEEERELATRLVEHGDTQAARKLIEA
                     NLRLVVKIAYEYRRAHKNLLDLVQEGNIGLIQAVSKFDPYRGVKLSSYAAFWIRAYIL
                     KFILNNWRLVKIGTTQAQRKLFFNLRKEREKLEQLGFQPTSALLAEKLDVPEKEVVEM
                     ERRLAAPEASLDAPLGTSNDDDGSRTRLDYLPSEDVRPDRAVAQSEFSELLRGKLEQF
                     AQTLEGREQTIFRERWLTDEPLTLQEIGDRYQVSRERARQLEKRMLDRLKKYLESELG
                     TSVDIGALSRD"
BASE COUNT         4524 a         9718 c         9726 g         4591 t
ORIGIN      
        1 cgtgccgcgc acgtgctcct cgtgcagctt ggtcagctcg cggcccaggc tggcgcggcg
       61 gtcggcgccg aacgccgcag cggcgtcctg cagcgtcgcc gcgaggcgat cgggggcttc
      121 gtagaagatc atcgtggcgg actctccccg cagcgtaccg aacaatgcgc gccgcgcacc
      181 cggttcgcgc ggcggaaacc ccacgaacgt gaatcgatcg gacggcagcc ccgaaccgat
      241 cagcgcggcc agcgccgccg atggccccgg cacgacctcg acgcgggccc cggccgcgat
      301 cgcggccgcg atcgcgtacg cgccgggatc ggacaccccc ggcatcccgg cctcgctgat
      361 caccgccact cgctggccgc cttgcagcgc ccgggcgacc tcgtccgccc gctcggcctc
      421 gttgccctcg aagttcgaga cgatccggcg cgccgggttg ctcgggccca gcgcgtcgac
      481 gtgcgcgagc aggtggcgcg ccgcgcgggt gtcctcggcg gcgatcaggt cggcctcgcg
      541 cagcacgcgc gccgcgcgca gcgacaggtc ctccaggttg ccgatcggcg tgccgacgat
      601 gaacagggtg ccgatcacgg cgctctcatc actacgccag gacggcgccc gtggccactc
      661 gatcggggcc ttccctgcgc ctccgccaga cgtcgtcgag cgggagcccg gtcggtctga
      721 accgcaagac ggcgcaggtc ttggacggat gaggaagcgc gcgagctcgg ccgcatcgcg
      781 agccgtgctc catcgcgtcc gctctgcttc agaagcgctc cgtggagact gctcgtaccg
      841 ggcagatagg gtagcttcgc ccaccgcggc gatgataggc tgcacgggaa cgagcaggac
      901 atgtcggttg ggaccacggg cagcggtgtg cgcacgttca ggggcgtcca cgtggcgatc
      961 acgggagcct cgtcgggcat cggcgaagcg ctcgcccgcg agctcggcgc agccggggcc
     1021 aggctgaccc tggtggcgcg tcgcgaggag cgcctgcacg cgctggcagc ggagctcgcg
     1081 gccggcgagc gcagcggcgg cagcttcgtc gctgccgtcg acctggccga catggagcgc
     1141 gcgacgtcgt ggatccagcc cgccgaggct gcgctggggc cgatcgacgt gctcgtcaac
     1201 aacgccggtg tccagcaggt cgctgccgcc gcggacctcg acgccggtga atgcgaatac
     1261 gtcctggcgc tcaacgtgcg cacgccgatg cgcctgatct gcgagttgct cccgagcatg
     1321 ctgtcgcggc ggcgcggaac gatcgtcgat gtcgcgtcga tggcggcgat cacgccgatg
     1381 ccgggcatgt tctattacaa cgcggcaaag gccgcgctcg ccaacgcctc cgaaggcctg
     1441 cgcggcgagc tgcggggcac cggggtgcac gtggtcaccg tctacgcagg accggtcgat
     1501 accgagatgt cgcgcatcgg cgcgagccgg ctggaggata cgctcgccaa gtcgctggcc
     1561 cccatgggga agcccgcgga gctggcccgg ctgattcgcc aggcgatcga gcgtcgagag
     1621 ccgcgcgtga tctatccgcg cgtgtactcg gtcggaacat ggttcccgtc ggtttctcgc
     1681 tggtgcaccg cgcgctggtc ccctgccccc aagcgccggg ccgggagctc gccggtgcag
     1741 cagagctcgc acatgcgacg aggagaaaga cgaggagaaa ggtgacatgg ccgccggggc
     1801 gcgccagcag caacgcagac tgggactggt cttctccggg gccggcgcgc gcgccgtgtt
     1861 ccaggtgggg gcgtacgagg tcctcgtcca ggatccccgg ttcgccggcg cagagttcct
     1921 gtcctgcacg tccggcgggt cgatcaacgc ggcgatggtc gcgagcggca agacgccggc
     1981 gcagatgatg gacttctggc tccggttcgc cgatacgccg cccgtgcacg cgaacaaggc
     2041 gttctttcgc gaggcggtgg tcacgctcgc caaggtgagc ctcccgttct ccagccggac
     2101 gcgcaccggg gccacgctgg gctggttgtt cggccgcctg ctccaccatc tctcacccga
     2161 cccggggaac ctgctcgcgc tgggcctcga gatcctgctc acccgccgct gggacctcgt
     2221 cgaccgcttc ctgacgcaga tgacggagtc gagcctcctc cacagcggtc cgctgcgcga
     2281 caagatcgtg cagggcatcg gcggcgagac cctgcccacc gggcggatcc acatcgcgat
     2341 caacacgatc gacgtcgcgt gttcggagcc ggtgcgcatc gtgacccagg aggcagcgcg
     2401 cgggctgcga ctcaacgatc gatacctcgt ggtgcccgcg atcacggtgg atcacgttgt
     2461 cgccagcgcg gcgctcccct tgcttctccc gccggtcgcg tatgcgggtc gctacctgat
     2521 cgacgggggc ttgctcgtga acagcgcgct cgccccggcg gtggacctgg gggcagacat
     2581 catcgtgccg atcctggcca tggccaaggc cgacgcccgc ctgtcgcagt acaagaatct
     2641 gaacgaggcg gtggagcgca ttgccgagtc gctgttcgaa tcccactacc agtcggatcg
     2701 aaagctcctg ctcgatcgca accggctcag cgggtccgcg cccggctccc agacgcggta
     2761 tcgcgaggtc aagctgtatc aggcgatccg gccaccggac agcagcatgt tcgacgtcgg
     2821 ctcgtacctg tacttctccc gcggactgct gggtgagatg tacgaggccg gcaaggccgc
     2881 cgccgcggcc tggctgaaac aaggcccgct cctggacagc ctccgtcccg agtccccctg
     2941 aacgagggca gcgccatgag cgacgtcgag atcgcgccgc gctggttcgc gtcccgctcg
     3001 ccagccgcgc ccgccgggtc gcgttcgccg atcggacgct cccggcggtc tcggttgcat
     3061 ggggcgacat cgtcagcgcg caccgcacca cggggatcgc cgagatcacc gtgtatctcg
     3121 ccgcaccgtg gtcggcgttc gtcgcgttgc aggcgatccg cccgttcgcc ggactgatcg
     3181 gacgcaccgc cgtgcagcgg tttctgaccc ggcagctccg gcgcgcgccg atcgggccga
     3241 gcgaggtgag ccgccggagc gcccggacgt ggtactgggc cgcggccgag aacgcgaagg
     3301 gccaacggat cgcggcgcag atcgatctgc ccgacgtgta cgacctcacc acgtccgccg
     3361 cgctcgcggc gatgcatcag gtgctcggcg ggcatgttgc tcccggcgca tggacgcccg
     3421 cgaaggcgtt cggggctggc ttcgtgaccc ggctgcccga cacgcaccta cggatgctat
     3481 cgtgatgcac gtcggcgtct gccggacccg cgtgaggtga ccgtgaagca ctacgacatt
     3541 gattcgctgg aaaaccggga tcctcgggcc atcgcgcgca tcttcgctgt cgccgagccg
     3601 atcttccgct ggtacttccg gccggacatc cagggactgg agaacatccc acgcggcgcc
     3661 ggtctgtacg tcggcaatca caactccgga gcgctggctc ccgacatgtt catcttcgcg
     3721 atggcggtcc accgcgtccg cgggatcgaa catatgccgt acgcgctcgc ccatgaggtg
     3781 acgatccgga tcccgctgat ccatcagctg atcgtcccgc tcggcgcggt gcgagcgagc
     3841 cacgacgccg cacatcgcgt gctggccgcc gggtcgaagg tactggtgta cccgggcggc
     3901 gacatcgatg cattccggcc attccgcgat cggaaccggg tggtgttcgg tgcccgcagg
     3961 ggatacatca agctggcgct gcgcgagaat gtcccgatca tcccggtggt cgccgaaggt
     4021 gcacactcca cgctgatcgt gcttcatgac gggcggtggc tggccaggac attgcgcatc
     4081 gatcgcctgt ttcgctatca agtatttccc gtcacgttga gtgtgccatg gggcctgacg
     4141 atcgggatgc ctctgccgca ttttccgctc ccggtgcgga tccgcatcag ggtcctcgaa
     4201 ccgatctacc tggatcgcca cggcgaggca gccgccgacg atcgcgacta cgtcgagcac
     4261 tgtcattgcc tggtccacag ccgcatggaa tcgacattgc agagcctggc gcaggaccgc
     4321 cgagcggcgg gatcgccgcg acgggagccg cagcgaagcg ggagtatcgg ggaacgtccc
     4381 ggattttcag gttaggtgca aaaccaccca gcatgacagc agaaacgatc tgccgaggct
     4441 gcatgtcaac gattgacgct ctcgttgggc atcgtgtacg gtcctcaacg gcagcgcgca
     4501 tggtggcggt gaatacatcg gtcgacgaga tcgtcgcgtt cgtgaagctc tacctcctga
     4561 gcgagttctt gccgggcaag actggagtcg cgatcgacag ctcgacgcga ctaaacaagt
     4621 ccggagtcct cgactccctc aagctggtga ggctggtgac cttcctcgag gagtcctatc
     4681 ggatcgagat ccagccccac gagatggccc ccaagcactt cgagacgctg caagcgatcg
     4741 ctgcgctcgt gcactccaag ctggtcatgc cggccgagcc ggcaccgtcg atcgaatgac
     4801 catgggcaac tattacctgg tttgcgcagc ggtgctggtc gcgacgtaca ccgtcaacac
     4861 cacgtacgtc acggtgttct accaccgcgg gctggctcac ggtggggtcg agctcaggcc
     4921 gggtgtgcgc aggttcgtgg cggctaccgg gagctgggtg accggactcg acccgaaggc
     4981 atgggcttgc atgcatcgcc agcatcacca gtacgcggac acggcgcgcg atccacacag
     5041 cccgcgcaac gtcgggatcc tgggtgtgct cgtcgcgcag ttcatcgcat ataaggcaaa
     5101 ccttgcactc ctgatcatgg gacatccgaa gtacacgaag atcgtggagg acatcgagtt
     5161 cccggtcagc tggatcaacc ggcgcagctg ggtgatcccg tacgccgtcc acctcgcgat
     5221 cgcgatcgcg atcggcgcgg ccgccggggc atggctgctc ggcgcggcct acttcatcgg
     5281 gctgatgagt cacccgatcc agggctggat ggtcaacgcg ctcggccacg cccacggcta
     5341 ccggaacttc gacacgccgg acggctcgcg caacaacacg ctcgtagccc tgctcgtcgt
     5401 cggcgagggc tatcagaaca accaccaccg caacccctgc tcgccgacca tggcggtgaa
     5461 gtggtacgag atcgacctcg gctacgtgct gtgccgcatc ctcggcctct gtggactggt
     5521 gacgattccg atctcgccga gcacccacca gccgagcggg tcggcgccat gaccgaagtc
     5581 gcggacttcg tccgcggcgt ggacacgctg gtggagctgt tgcagcaccg cgccgcggaa
     5641 cggcccgacc gcacggtgtt cacgttcctc gccgacggtg agctcgaggg cgtgcgctgg
     5701 agctacgcgg agctcgaccg ccgggcacgc gcgatcggcg ccgtgctgca agacgctgga
     5761 gcggccggcc agcgtgcgat cctgatctac ccgccggggc tggagttcct gagcgcgttc
     5821 ttcggttgcc tgtatggggg cgtcgtggcc gtccccgcct acccgccaga cccggggcgg
     5881 ctggcgcgaa ccctgccgcg cctgtgcgcg atcgcgcgcg acgcggagac cggcttcgtg
     5941 ctgaccacga atgacatcct gcggctcgcc agcgacgtga caaacgccga gcctgacctc
     6001 gcccggctgc gctggatcgc gagcgacgcg gtgccggccg gatcggagtc ggggtggcgc
     6061 ccgccgggcg tgggccgaga ctcggtggcg ttcctgcagt acacgtcggg ctcgaccgcc
     6121 gcgcccaagg gtgtgatcgt caggtaccgc aacgtcatgc acaaccagca catgatccag
     6181 cgccaggcgt gccgggctgc atggctcgag ggcaccctgc agtgaccggg gtcagaaccg
     6241 gtacagcgca cagccgacct ggatcccgga cccgacgccg atgatcaggc ccaggtcgcc
     6301 gggtcgcacg cggccgctgt cgcggacttc gcgcatcgcg aacgccaggg acgaggtgaa
     6361 cagatcccgg cgatcacgga ccgcatcgac catgcggtcg gccggcaggt ggagcgcgtc
     6421 ggcgagccgg gtgatgaacg acgacgagat ctgaggcggc agcatccacc ggacctcgga
     6481 cgcaggcagg tgcgcgatcg ccagcgtctc gtggacagca tccaaaatgc agcgcaggta
     6541 cgcgctctcg agatcgggat cggcctggac ctgaagatag atcgcagatc cgttgcaatg
     6601 cgtatgcgac gcgagcgcgt tcagatatgc cgggtaatcc ttgaagtgga agtcgatgaa
     6661 gccggtgctg ccgtcgctcg actcgcggag gacgaacgcg gacccggtct cggcgatacc
     6721 gcgcagtcca cgccccgggt cgtccgcgtt gttctcgatc tccgcggcga ccaccatgac
     6781 gttgtcgcgg tcgccggcgc gaagcagctc gacggcgaga tggcacgcgt tcaaggtccc
     6841 caggccgccg ttgaggacgt cgaacgcgag ggtcgtacgc gaatcgtcgc cgtcgacgtc
     6901 atcgttgatc ccgagatcgc cggcgagcaa ggacgcgacc gctggctcgc acaggtagtc
     6961 ctcgcgatac accccggtgt agagcagcag gtcgatgtcg cgccggcgga ccgccgactc
     7021 gcgaaggcag ttctgcgccg cgtggcggcc gaggtccacg gcgctcctcc ccggcgtgat
     7081 cgtggacggg agcagcccga tgctctcgat gcacatgccg ggtcgccgag ccgcggccgg
     7141 cgcgctgcgc ggcgcgggag gcaccgccct ggctcgggcc ccgggcgcgc cctgcaggat
     7201 ccggtcgggc aggtcgtcga gcacgtacag cgcggtgccg agcgtcagcc cggatcccga
     7261 caagccgaag acgatccgct cgcccgactt gatctgtccg ctgcggatct ggtccatgag
     7321 cgcgacgaag tgcgtggtcg tggcggtgtt gccccgctcc ttcaggttca gggtcagcac
     7381 cgcatcggtg atgacctcgc gccccgcccg ccgattgatc tcccgggccg cttcgcgaag
     7441 ggtcgtgttg gaggtctggt gcaggaccag gtgcgcgatc gagtccagcg accagtcgtt
     7501 gcgctcgagc gtggccaggg aatggctgat tccgccctgc agcgcgaccg aagacagctt
     7561 cacggagtcg gtgtgcatga tcgcgccggg gctgtcaccg ccggtcgcct tggcgatgca
     7621 gtacgggctg tagcggccca gcgtgtagag ctcgatgtcg tgaaagccgg cgtccacccc
     7681 gggtgcggtg gacctggcga gcatgacggc cgcacccgcg tcgccgaccg tcaggcacgg
     7741 tagccgcgga tccatgaaac cgtcgatctc gcgctgcgcg gtccgggtga tgtgcgtgat
     7801 gtactccccg ctgaccacca gcccgcatcg gatcagcccg cgccggagga acgcgtcgac
     7861 cagcaggatg ccggtccaca tgccggtgca cgcgttcgac aggttgaagg cgatcgcgcg
     7921 gtcgagcccg agctcgtgct tcaagcgcag cgccgttccc ggctcgaacg tgaagcgcac
     7981 ctcgggaccg tcgtatcgcg agatgttgca gcagatgagg agctcgacct cgtccggacg
     8041 gtaccctgag cggctcaggc agtcggcgat cgccttcctc gcaagcgtga cggcgaactc
     8101 gccgtcgccg acgacccgcc gcgatcggat cccggtgaac gcctccagcg ggaaccgcac
     8161 cggcttcttg caatccctca gcacctgctc ggtggtctcc tccctgggag gcaggtacac
     8221 gccgaggctc tcgatcaccg ttctggtccg cgccatcggc tcgaggtcct ccggcgcggc
     8281 gtctgtgtgc ggggtgctcg cctcgttgct cgcctcgatc gccgccgcca gctcggcgat
     8341 ggtctggtac tggaacacca tcgcgggcga caggtagatc ccggcgtctc gcgcgtccac
     8401 cacgacctgc aggctcacca gcgacgcgcc tccgaggttg aagaagttgt cgtggactcc
     8461 caccttgtcg acgtggagtc gcgaccgcca gatctcggca agccgctgct ccaccgggtt
     8521 acgcggcgcc acgtagcact cgcgcgccgg agcggccggc ggtcggtcgg ccagcgcgtg
     8581 gcggtcgatc ttgccggtcg ccgtcaccgg cagcgcgtcg agcaccgcga accgcaccgg
     8641 gaccatccag cccggcagct tgcgcttcgc aaactcccgg aggcggcgcg cgagatcgtt
     8701 ttgctcgggc gccatcctct caccggccgg cgccgcgggg accacgaagg cctcgagctg
     8761 atgcccgccg tcgtgcccgc tgcgcgccaa caccgcggcc gcgccgatgt cggggtgctg
     8821 ccgcaggacc atctcgatct ctccgagctc gatgcgcatc ccgttgatct tcacctggtc
     8881 gtcgtcgcgg cccaggaact cgaccgtgcc gtccgcgagg tagcgagcga cgtctccggt
     8941 gtcgtagagc cgcgcgtcct cgcgacggtc caagaacgga tcggcgatga agcacttctc
     9001 ggtgagctcg ggccggttca ggtaaccgcg tgcgaccccg acgccgccga cgtgcagcct
     9061 gcccggcaca ccgatcggca ccggctgctt gagtgcatcc aggatgtaga tccgcaggtt
     9121 cggaaccggg cggccgatcg gcaccggccc ggtcccggag cggacccggg tccacgtggc
     9181 ccacacggtg cattccgtcg ggccgtagag gttccacacc gcgctgccgc aggtgagcag
     9241 ctgctcggcc atgtcgcgcg gaagcgcctc gccggtgcag aggatccgca gcccgggctt
     9301 gccgcgccag cccgccgcga gcagcaggcg ccagccggcg ggcgtcgcct gcatcgtggt
     9361 ggccccgcag cggtccagca cgactgccag cctcgatccg tccgtgacgt ccaggtcctc
     9421 ggccagcacc accgtcgccc ccatggacag cggcaggaac agctccacga cggagatgtc
     9481 gaacgcgagc gtcgcgacgg acaggaacac ctcgtgcgag ccgaactcga gcgctctctg
     9541 catcgcggtg atcagattga ccaccgcgcg atgcgcgatc gccacgccct tgggctgccc
     9601 ggtcgacccc gaggtgtaca tcacgtaggc gatgtccgtg cccgagcctg ccgtccgggc
     9661 gcgcgggggc tcgtcggccg ggccgtcgag caggccggca tcgacgtgga ggacgcgcat
     9721 gccggctccg gcgacgaggt cgccgacgag ctgcgagcac gtgaccagca ggcacgcccg
     9781 gctgtcctcg agcatgaacc gcacgcgctc ggccgggaga cggggatcca gcggcaggta
     9841 cgcggcgccc accttgagga tccccagcag gcagatgatc gtctcggggc tgcgctcggc
     9901 gtgcagcgcc aggagggcgc cgcagccgac gccctcggcg gccagccggc tggcgacccg
     9961 gccggccttg cggtcgagct cgccgtaggt catgcgccgg ccggcatgga ccagggcgac
    10021 ggcatcgggc gcgtgcgcgg cgctggcctc gaacagctcg tggacgccgc cgtcgcggag
    10081 ctccgcgtgg gtcgcgttcc aggtcgacag gatctggcgc cgctccggct cggtcaggag
    10141 cggcagtctc gcgatcggca ggtccggatc cgcgatcagg ccgtgcagca tggccgtgaa
    10201 gtgctgcagc atgcgggtga tcgtcgcggc gtcgaacagg tccgtgttgt actcgatcgt
    10261 gttcaccagg ccgtgctcgg tgtcggtggt gaaccacgtc agatcgtact tcgaggtccc
    10321 gctgtgcagg tcgagctgcg tcagcgtcag cccgggcagc gcgagcggcg ggagcggcga
    10381 gttctggagc acgtacatga cctggaacac gggagagcga gacagcgcgc gctcgggctg
    10441 cagatccttg accaccacct cgaacggcac gtcctgattg gcgtacgcct cgaccgcgct
    10501 ggcctgcacc cgccgcagca aggtccggaa cgacaggtcg cctgccagct cggtgcgcag
    10561 taccagaata ttgacgaaca tcccgatcag cccttcgatg tcgggatgat tgcgattggc
    10621 gatcggcgtg ccgatcagca tatcggtctg gccggtgtag cggaacagca gcgccttgac
    10681 cgcggccagc atggccatga acagcgtgca gccttcccgg ctgctcagcg ccttcagcgc
    10741 ggccgacaca ctttcgggca gcaccgcgga cctggtgtcg ccgcggtagg tctggtgggg
    10801 cggacggggg cgatcgccgg gcagctccag caccgggagc cggctgccga gccgggccag
    10861 ccagtagcgg cgctgcgcgc cgagctccgg gctatccagg cgctggctct gccacagcgt
    10921 gaagtcggcg tactggatcg gcagcggcgg tagcgtggcc gtaccgccgc cggcgaacgc
    10981 ctggtagaag ctgccgagct cctggacgaa cacccccatc gaccagccgt cgaacacgct
    11041 gtggtgggtg acgaggacga accagtgctc ggagtggctc agccggatca gggtggcgcg
    11101 cctcagcggt ccggactgca ggtcgaacgg gcgttgcgcc tctgcgagcg catggcgccg
    11161 ggcctcggcg atccgggcat ccggagcgag ctcggtgaga tccacgaccg gaagcgcggt
    11221 cgcatccacc ggccggagca cctggaccgg cagctccgcc ggcatggcga acgtcgtccg
    11281 caggacctcg tggcgctcga cgatcgcggc gcagctgcgc tcgagcgcgc cgatgtcgag
    11341 ccggccgtcc agccttgcgg cgtagatcag gttgttggcc gacgtgccgc cctccagctg
    11401 ctccatgaag tagatccgct gctgcgggaa cgaggccggg accgcgcccg gtggacgccg
    11461 ggggatggcg ggtgcagcgg gcgcgtcgtg gccgcggctc cagcgctcca gcagcgcacg
    11521 cttggcgggt gatagcgccg tgccgacgga tcgcctggtc tcttcgctca cggtcccgtc
    11581 ctgtcctgcg gagaccgagc cagcaggcgc agggcgtcct cctcgctcag cgcgtcgagc
    11641 ttttgcatga gctcgctctc gaccagctcc gccatggtct cgatggtgaa cgaccgataa
    11701 cacgccgtcg gatcgatccg gacggcgaac gtgcggttga tctgattgag gatctgcccc
    11761 gccagcacgg agtcgccgcc gagctcgaag aagctgtcgc gcgtaccgat cccgtcgatc
    11821 gcaaggacgc gctcccagat gccctggatc gtgcgctgcg tggcggtctc gggctcgacg
    11881 tacacctgct gctcgctacg gcgtacacgg gtccgatgcc cggcctccac gccgccgacc
    11941 gcccccggct cctgacccgc aatctcggcg agcatggcgg atccggttcc cagcggatag
    12001 aactgcagca tgttgcaaag gtcgtagggc aaagccatca cctgcgcgat gtcctctcgc
    12061 atcagccgct cgagcgtctc ggtccccgac gatggagaca gcatgcggac gaaccgcgcc
    12121 gccaccagcc ctcgcgcccg caccttcccc gcggcggccg ccgccagacc gacctccgcc
    12181 cagggccccc agttgatgct cagcgccggc accccctgtg ctcgtcgaag atgggccagc
    12241 gcgtcgagga agacgttcgc cgcggagtag ttgccctggc cgggggaccc gagcaccgac
    12301 accgccgagg agaacagcac gaagaaatcc agcgcagcgt cgcgggtcag gcgatggagg
    12361 ttccaggcgc ccgcgatctt cggcgccatg acccggcgca tccgggcgcg gtcgaacgcg
    12421 accagcgcac cgtcgtcgag gatcccggca gcatggacga tgccgcgcag cgggtgggcc
    12481 tggctggaca gcgcgcgcaa cagctcggcc atcgcgtcgc gatccgtgac atcggcctgc
    12541 gcggtggtga cctcgacgcc gtcgcgccgg agcgccgcga tgacgttctc cgcggtgctc
    12601 gacggaggac tcctgccgat caggacgagc tgccgcgcgc cctggctcgc catccactgc
    12661 gcgacgacca ggccgagccc tccgagccct ccggtgatcg cgtagatcgc gtcgggccgg
    12721 acgaccgcgg agatgtctcg cagcgagctg ggctgccgcc ggctgcgtcc cgtcacgtag
    12781 ggcaccacac ggggcacgtg cgccgtaccg tcgcgaaccg cgatctggtc ttcggcgtcg
    12841 gccgagccca gcagccctgc gagttggacc gcgtcgccgg gaccagccgc tggatcgaga
    12901 tcgatgcgcg agcaccgctg ctcgggatgc tccagcgcga tgacgttggc caggccccac
    12961 agcgggctct gcgcgatcgc cagggagccg gctgcggcgc ccaccggttg cgcgcctcgc
    13021 gtgacgagcc acagacgcgg cggcgggtag gctccggact cgacgagccc cgacaccacg
    13081 ccgagcactg gatcgcagat gtcgagcgag gtctccagtg catccgcgtg gtccggatcg
    13141 gcggcgtcca gcccccacag gtagacgatg ccccgccagc gcgtgtcgcc ggacttgatc
    13201 tgctcgagga tcagcggatg gatcagctcg cggccgggtg gcgtcgtgat gtcgcggccg
    13261 ttcgccgcga cttggcgggc tccaccggcg aacaccgtga cgcagcagta gccgcggttc
    13321 tccagcaggc tgaccagccc gagcccgacc cctccgcgat cggcgaacac cagccagcga
    13381 tccccggact gctctgccgc tccggccggc tcgacgacgt cgtcgagccg gcggtgcgtg
    13441 cgccacgcgg tctcgtagag ccaggtatcg cgcttgcggt gatcgatctg cagggcgccg
    13501 cgcgcgatgc gctggagtcg cacgccctcg agctcggcga tcacgcgccc cgagtcgtcg
    13561 agcaaccgga cgtcgacttc gaaggcgatc gcgctgtggt cgagcgcgcc gtggcgcatg
    13621 acgtggctcc acagcgccgg gcccggctcg aacagctgga tccgcttgca cccgaccggg
    13681 acgaacatgt cgagcgcatc gctcgccacc tgggtcgccg acagcacctg caggcaggca
    13741 tcgagcactg ccgggtggat tcgataggct gcgagctcgt cgtgggagat ctcggggatc
    13801 tcgatgcgcg cgagggcctc tccttctcgc cgccagatct gctttacccc ccggaaggtc
    13861 gctcccagct ggagcccccg gtcccacagc cgctggtaga acgcctgggg cgcgatcgat
    13921 tcggagcacc gctgctcgat ctcgtcgatc gatgcccacg cgggcggccg cagcgagcga
    13981 tcgatctcgt cgcgaacgag cgtcgcggtc atatggtgcg tccagctctc gcgcagcgag
    14041 cgcttcgaga ccggactgct gaagacattc agcgaccagt ggtccccggc cgagggcgcg
    14101 atcgtgacct ggaggtgcct cggctccttg ccgtcgaggc tcagcgcggc cagcaacgcg
    14161 acgtccgagg cggtatatct ccgggccatg ccggactgct cgagcgcgga cagcgacatc
    14221 tcgaagaagc ccgccccggg gaacacgacc tcgccgtaca cttgatggtc cgccacccag
    14281 ctcgggatcc ggcgatcgat ggtcgcctcc cagaggtgaa ctccctcggg gtgcatcagg
    14341 tgggtgtggt gctggagcag cggatgcccg ccctcgccgg gctgcgagcc ggatggccgc
    14401 gccgggcggg cgttcgcgcc gttcaccagg tcgccgagcc agaaccgctc ccgctgccag
    14461 cagtaggacg gcagcggcgc cggcaccccg ccggacgggt agacgccgtc ccaccgcacg
    14521 gcgtggccct gcgcgtagag cgcggcgagc gagcccagca gcgtagcgac gccgtcgtcg
    14581 ccgcgttgca gcgaggcgac cgcctcgccg cgcacgccga ggtgcgccag cgtctgcgcc
    14641 accgctctcg tcagcaccgg atgcggaccg atctccacga acaccggagt ggcctcggcg
    14701 gccagctgct cgatcgcctc gaacagccgg accggctcgc gcaggttgcg cccccagtac
    14761 gaggcatcca gcgcgtcgcc cgcgatcggc tcggcggtga ccgtcgatac catggtgagc
    14821 cgggccggac gcggctggag gttcgcgagc gccgttgcga gctgctcgct caccggctcc
    14881 atgaacggac tgtgcgaggc gtagtccacc gcctcgagcg tccggaccgt cacaccgtcg
    14941 agcgacaacc gggagacgag ctccgcgagc gcctccggct ggccggagat caccacgctc
    15001 tcggggccgt tgtgggccgc gacaaacagc tgcccgggat gggcgtccag cgcctgcctg
    15061 acgcgcggca gcgggaggtc gaccagcgcc atcgcgcccc tgcctgatgc gcgcttgatc
    15121 gcgcggccgc gctcggtgat gacccgcgcc gcggcgtcga gctccagcgc ccccgcgatg
    15181 tcgctcgccg ccacttctcc catgctgtgc ccgatcacgg cgtcgggctc gatcccccac
    15241 gatcgccaca gctccgcgag cgcgacctgc accgcgaaca gcgtcggctg cccgagcacc
    15301 ggatccacca ggacgccggc cgcctcgtcg tgctggatgt gatccagcag ccggagctcc
    15361 ggctggtgcc ggcgcagggc gagctcgacc cgatcgagct cggcgcggaa caccggctcg
    15421 tccagcaggt cgcgggcgag cggccaccac tgggggcctt gccccgagaa cacgaacacg
    15481 aggcgccgag cctggccggc ggcctcgcgc ccggcgtgca cgccggggta gagttgcccg
    15541 gcggcgaagc cttccagcag ctcggcgagg tcccggctcg atcgacccac gacggcgagc
    15601 cggtgctcga ggtgcccccg ccggacgctt gccgagaagc acacgtcgtg cagcgctccg
    15661 ggcgcggatg acaactccgc gagcgcgcgg gcatgggcgc gggcgagctc acggagtgcc
    15721 tgcgggcttc gcgccgagat cgggagcagg tggtaggcgc gggcaggctc ggcgcggcgc
    15781 tcggcggccg cgggcgggcc ctcgatgatc acgtgcgcgt tgctgccgcc gaacccgaac
    15841 gagctcacgc ccgcgagcgg actcccggtc gccggccagg gcgacgccga ggtctggacg
    15901 cggaggcagg tatcctcgaa gcggatgctg gggttcggag tccggaagtg cagctggagc
    15961 gggatgaccc cgtggttcac ggcgagcgcc accttgatca tgccggcgat gccagcggcg
    16021 gcctcggtgt gaccgatgtt cgtcttgacc gagccgatcg cgcagatcgc gcccgccggc
    16081 cgatccgtcg agagtacggc gtggagcgcg gccgcctcga tcacgtcgcc caccgaggtg
    16141 cccgtgccgt gggcctccac gtactggatg tccgccggcg agatgccggc atcgcggcac
    16201 gccgcccgga ccaccgcctc ctgagcctgt cggctcgggg ccagcaggcc gttggtccgc
    16261 ccgtcgtgat tcaccgcgct gccccggacg atcccgtaga tccgatcgcc gtccgccagc
    16321 gcgccggcga gcggcttgag caccacgacg ccgccgcctt cgctgcggac gtagccgtcg
    16381 gcggcggcat cgaacgcctt gcaccgccca tccgccgcca tgacgccggc cttggcgaag
    16441 ccgatcgcga tcgcagggga gaggatcagg ttgacgccgc acgccagcgc cagcgtcgac
    16501 tctcccgact gcaggctctt gcacgcgtgg tggaccgcga ccagcgacga cgagcacgcg
    16561 gtgtcgaccg tgatgctcgg cccgtgcaga tcgaacaggt acgagatgcg gttggccagg
    16621 atgctcagcg cgtttcccgt gccggcgaag ttatcgacca gccggaggtc gctgagctgg
    16681 atgccactgt actcgttgac cgaggcgccg acgaacacac cggtccgcgt gccggcgagc
    16741 ttccgcggca cgatgccggc atcctccagc gcctcccagg cgagctcgag gatcagccgc
    16801 tgctgcggat ccatgcgctc ggcctcgcgc ggcgagatgc cgaagaacca cggatcgaac
    16861 tgatcgatct ggtcgaggaa cccgccccag cgcgtgttgg ccttccccgg cgccatcgca
    16921 tcccggtcgt agtagtcggc ggcgggccag cgctgctcgg gcacctcgcg gatcgcgtcg
    16981 acgccatcgc acagcagccg ccagtacgcc gcgggaccgc gcgcgccggg aaaccggcag
    17041 ctgatcccga cgatcgcgat cggccccgac gcaaccggct gcggctggac ctcggcggcg
    17101 ggctccggcg cctcgttgtc gccgaggtgc gccgcgagcg ccgcgatgca tggatagtcg
    17161 tacgccaggg tcggcgacag ccgccgtccg aaccaggtct cgagctcacg cgagatgtcg
    17221 atcgccatca tggaggtcac gccgtagagt gcgaacggcg cgcgcgggtc gatctggtcg
    17281 gcgtcgatgc tcaacgcgcg agacagtcgg gtggtcagcc aggcctgaat gtcatgtacc
    17341 ttcatctgcg ctcctgaggt ctgtaacgtg agcttggtga gcatgtgcca ggctcatgca
    17401 cgtcgcgctg accactgagg aatgctcccc ggcacgcctg ctgccggatc gctacttggc
    17461 ggggtgtccg cctacgacat gcagcttcca tagcgcagtc gcattcatgg agcggttacc
    17521 ggacatccga ccgtggtcca cgttggcctg catcatgagc gcctggtgcg attcgaccgg
    17581 aggcacctcc agcatgggct ccatcggtac cacgttcgca cgggcgcgat ccgtgtacag
    17641 ctcgagcgcc tcgcgcttgt tccggtccag cgcgtcgtgc cggtccttga agctcggaag
    17701 caccttggtt ccgaatagct ccagggactc gcagacgtgt tcgtggcggt aattgccgct
    17761 ctgctggagc aggatgagct gatccacacc cgcggcctcg aaggtcgaaa agtagtcctg
    17821 gatcttcgcg ggactgctaa tgccgccggt tccagccatg acgaacggag ggagtttctt
    17881 gaactcctcc cacatgtcga gacgacccgg cacgtgcgtg cccgtccggt agtgattgag
    17941 accgtaggcg aagtagcgta gaccatcgag accgcgctgc tgcgcgatgt cttcgtcctc
    18001 gtggcacatg aagccggcca gcatggcgac attgggattg ggttcgatac cgatcggcgt
    18061 gcagtggttt ctgaacacct cgtagtactc ttcgacccag aacttcgctt cccgggcatc
    18121 catgaacgca aacgtcagcg cgcccatgcc gagccgcgcc gccagcttca tggtgtcgcg
    18181 gttcgagcac gcgagccaca gcgggggatg cggcttttgc aacggcttgg gcacgatgtt
    18241 gcgacacggc atcgagaaat attgcccgtc gaagccggga tagggctcca tgccgaccat
    18301 gcggaccgct tcgcgaaccg cttccatcca catcgctcgc ttgtcgacgt agtcgatacc
    18361 gaatgcttcg agctcgagtc gcgaactcga ctcgcccgtg ccccagtcga ccctgccgtt
    18421 gctgatcaga tcgagcgtcg cgatgcgctc ggccacccgc gccggatgat tgtacgcggg
    18481 tggcatcagc acgacgccgt gcccgaggcg gatcgtcctg gtacgttggc tgcacgcggc
    18541 gaggaagact tccggagccg aggaatgcga gtactcctcg aggaagtgat gctcctgagc
    18601 ccacaggtag tcgatcccga tccggtctgc gatctccgct tgctcgaggg catcgttgaa
    18661 gaatgtccgc tcgtggtcac cagaccatgg acgcggaatt tgatgcgcat aaaagattcc
    18721 gaatttcata ttcagagcac ctttatccat ggatgataca atgccgtctg gccgccgtcg
    18781 cgctcgggta ggctcgcgaa tgttcggacc tcgagctcgg gattgtcctg gaaccaggcc
    18841 gccctcgctg cgccgccccg gctcccagac ggcccggcaa tctccacgag ccacgccgca
    18901 tgggcgccga aacctcgggc taaccacccg cccgtagcgg gtaccatcgc tcctatatcg
    18961 acacgagcta ccagcatccc gtgatctcgc cgatctcgaa cctgcggctc gcgaacgtat
    19021 tcttggcgcc ccgaggtttc aagtcatggt ggggcaaatt ccgatccggg acgcagatgc
    19081 gggattactt ccgatcacga gtgcgcggat gaagatagac gagactgtag aacatggatg
    19141 ttattttgca gcagaagaac tcgcgaagaa ctcgccgcac ccctgctggg cggcagacgt
    19201 gagcctaccg tgagaacttt gcagcagtca gggccgatca ctcgacagta ctgtgctggt
    19261 tctttgtctg gatcaatttt cgatcggata gaaaattcac catcgagaga tcgatgcagg
    19321 atcaactgtc actacttgtc cttgacgaag atcactccca tcgaattatt ccaagcgagc
    19381 actgttccga agtaactctg cactcctgcc gtgcgagtgg tcggtgcgtc aacgcgaagc
    19441 gccttgctgt ctcgggtatc ggctggggca ctcccgccat ccggattcat ccggattcgt
    19501 ctatcgagca tgactctcta cgcgcaaaaa atatattcag agtacaatca gcgattggcg
    19561 cggttactgc tatggattcg tccgcaggtt cagcaggtgt gattctgcag ctctcgacaa
    19621 tgacccaaac cgaaggcctt ggcgatgaaa gtcatcgcgg gacacaacat tcggatcttc
    19681 acgccgttga caggatgagc tgagccgtgg cagcgtcggc ctgttgatag gcgctgtctc
    19741 tcaccgctgg ttgggaagac cgtcagattc cttcccgacg tgatcgattg cggctcaggc
    19801 ttgctctggc acaggagtcc gatgtcgacc atgtctacgg tcaataactc ttgatggtga
    19861 ccgacaccgg aggcaattgg ccatggatgg tgatttcagc acgtgggttg atctggttca
    19921 attccgggca cggacgcaac ccaaggacgt tggctacatc ttcgtccggg acggcacgac
    19981 cgaagcacaa agccacactt acgaggagtt gcaggcgcgg gctgtcgcga tcgcggccca
    20041 gctccaggcg actcacgctc ccggcgagcg cgcattgctc ctctatccac ccgggctcga
    20101 gttcatcccg gcgttcttgg gctgcttgta ttcgggagtg ctcgcggtgc cggcatatcc
    20161 ggcgcttcgc gcgcagaacc tgcctcgcct cgaggcgata gcggccgact cgcaggcaac
    20221 ggttgcgttg acaacgtctg gcctgttgga tggcccggcg cgccgcagtc ctgcgctgga
    20281 aggtctggac tggatcgcca gtgatgcctc cccgccgagc ccgggggggt cgtggacccg
    20341 acctgcgctc gatggtgaca gccttgcgtt cctgcagtac acctccggtt ccaccggtac
    20401 gcccaaaggc gtgatggtga gccatggcaa cctgctccac aaccagcgca tgatcgagca
    20461 ggcgttcgac catgaccagg acacggtcgt cgtgggttgg ctgcccatgt tccacgacat
    20521 gggattgatc ggaatcgtgc tgcagccgct ctacctgggc cggccctgca tcgtgatggc
    20581 tcctacggac ttcgtgcagc agccgctgcg atggctgcgg gccatctctc attaccgggc
    20641 caccacgagc ggtgggccga gcttcggcta cgggctgtgc gaacggaaga tctcccccag
    20701 cgaggcggcc agcgtggatc tgcgtagctg gcggctggca ttcaatggat cggagcccgt
    20761 ccgcgcggac gtgatggatc gcttctgtga acggttcgcg tcgagtggct tccagcggaa
    20821 ggcgttttac ccatgctacg ggctggccga agcgacgctg ctggtctccg gcggttcggc
    20881 atcacgagct ccggttgtcc agaccgtgcg agcgagcgca ctggagcgtc gcaagttcga
    20941 gcccggagta ccgggctcga tgctcgtagg atgtggtcag tcgtggctcg ggcagaccat
    21001 cgagatcgtc gacccggaga cccgggtgcg atgcccaccg ggtcagatcg gcgagatctg
    21061 ggtgcgcggg ccgagcgtga cgaagggata ctggcggcga cctcgagaca cgcaagagac
    21121 gttcggtgcc cgtctcgagc agggtcgtga tgcatttcta cgctccggtg atctcggagt
    21181 cgtccacgat ggcgagctat tcgtgaccgg cagggtgaag gacatcatca tcatccgtgg
    21241 gcgcaatcat tatccccagg acatcgaggc cacggttcaa cgcagccacg ttgccttgcg
    21301 cgcggactgt ggggcggcgg tggccgtcga gatcgacggc gaggagcgcc tggtcgtgat
    21361 ccaggaggta gagcgagagc atctgggcca gctcgacacg aaagcactga tagccgatgt
    21421 ccgagaggca gtggcctctc agcacgatat tttcgtgcac gcgatcgtgc tcttgaagcc
    21481 tggagctatt ccgagaacct ccagcggcaa agtgcaacgt catgcttgtg ccacggcctt
    21541 catggcagga cagctggcag tcatggcgcc ggagatagct ccatgacacc cgatcaacct
    21601 gtgagtctcg ctcccgcggc ggccctgtac cctgagctcg acatccccgg ggatctacct
    21661 ggggtgagcg gtatcgccaa gcctgggatc cgcgataccg ccacggatag cagatcggac
    21721 aaggtgttcg ccgtgatcgt gacgttcctt cctccgctgt ccgtcgcgct ggcggtgatc
    21781 ctactgtact acggttggta ccagcttacc tccatcgagg tcggcctgat gctgggaatg
    21841 cacttcctgt gcctggtggg cgtggaactc ggattccatc gcctgttttc ccattgctcg
    21901 tatcgggccc accggcaggt gcgcatgaca ctcgcagcgc tgggctccat ggcattcgag
    21961 ggaccggtca tctggtgggc tgccacacac cgcaggcacc accgcctcag cgacaagcct
    22021 ggagacccgc actctcccca tctgcacacc gggctgtggg ccggctttcg cgggttcgtc
    22081 cacgcgcaca tcggctggtt ctggatcccg tcgagcatgc ggccgcccgg atgggctcgc
    22141 gcggtgacgg atctctatcg cgacccggac atcttcaaga tccacatgca ctacttctac
    22201 tggctggcag cggggtttgc gttgccggca gcgctcggag gaatcctcca tggctcctgg
    22261 aagggcgtct tgctcggcct cctgtggggc gggccaatcc gcgtgttcgc gatgaaccac
    22321 ctgacctact ggtgtatcaa ctcggtcacg cacagccggt ttggcagccg tccgtttcgc
    22381 acgacggacc gcagcagcaa tgttccgctc ctggcgctgc cgacgctggg ccagagctgg
    22441 cacaacaacc accatgcgtt cccgagctct tccaccacgt ccatcgcctg gtggcaggtc
    22501 gacacagggg actggattct tcgtgtattg aaccgcctcc acctggtctg ggaacgaaag
    22561 ctcccgacgg aacagcagcg ggccaggaag gcgagaaggg acgaacagaa accatgacgg
    22621 aagcagtgca accggtgatc catgcagagg caagcctccg tgagaaggag atccaggact
    22681 ggctgacctt gtcgatctcg cgcttgattc atgtcgatcc aagcgatctg gacgtgaatg
    22741 tttcgttcga ccgctatggc ctggactccg cggcggccgt ggagctcaca ggcgacctcg
    22801 agcgttggct cggccgcaag ctcacgccaa ctctgttcta tgactatctc accatccgtg
    22861 cggtgagcgc ctacctcgcc gtggctggtt gaacgcgcat gacgacgcca acatctacgc
    22921 ggctggacag ccccggggcg tccctcgaag ccatccagga tcactacgac gtcagcaatg
    22981 atttctaccg actctggctg gacagcaata tggtgtattc gggcgcgatg tgggagccgg
    23041 gcgacacgct cgagacagcg cagctgcgca agatcgatta ccatatcgcc caggcaaagg
    23101 cggccacgag caagcgcgtg ctcgatgtcg gctgcggctg gggagcggtg ctcaagcgac
    23161 tggcacacac ccacggcgtc gaccatgtcc tcgggctgac actgagcgca gcgcaggcgg
    23221 agtgggtccg cgggctcggc atcccccaga tcacggtcaa ggtggaaagc tggaccgatc
    23281 atgttcccgg ggcgccctac gacagcatca tctcgatcgg cgcattcgag cacttcgccc
    23341 ggctcgagtt ctcggaggcg cagaaggtcg cggcgtatcg caacttcttc gcctggtgtc
    23401 aggagcgcat ggtcccgggc ggctacctgt cgctccagac ctttgcctac ggcaacacgc
    23461 gcagccgcga agaggcgatg aagatgccgg cgacccagtt cctggcgaac gagatcttca
    23521 aggagaccga cccgccccgg ctggccaaca tcgccgaggc taccgagggt agcttcgaga
    23581 tcgtctcgct gcgcaacgac cgggaagact atgcccggac ctgtcgcgag tggctggccc
    23641 gcctgcaagc ccggcgagag gctgcggtgg ccgtggttgg agaggaagtc gtcgcgcgct
    23701 acgagcgcta cttgaagtac tcgttcatcg gcttcgagac cgggaacctg gccttgtttc
    23761 gtatcacctt gcgcagtctg aatacccgct ggaggaaacg atgaacgatc cgggcgccgg
    23821 gccggcaatt gccattgtcg gatgggcatg ccgattcccc ggcgcgagct cgcccgaggc
    23881 gctctggcag ctgctcgcga ccggcgcgga tccgatcggc gtggtcccgc cgaaccgatg
    23941 ggacgccgac gcgctgtacg ccccggcgcc gcgtactcca ggcaagatga acacccggtg
    24001 gggtggtttc ctcgacgacg tggacgcgtt cgacgccgag tttttcggga tgcagccccg
    24061 ggaggcgtgc gcgatcgatc cccagcagcg gatcgcgctg gaggtggcgt ggagcgcgct
    24121 ggaggatgcg gggatcgtgc ctgcgcggct tgcggggacg tccgtgggcg tgttcttcgg
    24181 cgcgagcaac tgggactaca acaacatcct caaccgagac atcgcggtcg cggaggagca
    24241 ttccggcccg gggacggcgc tcagcgtgat ctcgaaccgt gtctcgtttg cgctcgatct
    24301 gaagggcccc agcatcacgg tggacacggc ctgctcgtcg gcgctggtcg cgctacactg
    24361 ggcgtgtcac agcctgcgta cgcgagagtc gagcctggcc ctggtgggag gcgtgaatct
    24421 gatcctgtct ccggagacat ccatcgtgct gtcgcaggga cgggccctgt cttcggtcgg
    24481 caggtcacgg acattcgacg agggcgcgga tgggtttgtc aggagcgaag gatgcgcggt
    24541 gctggtggtc aaacggctcg accaggccga ggcggatggc gaccggattc tcggcgtggt
    24601 ccgcggcacg gcggtcaatc acaacggcgc gagctacggg ctggccgccc cctacgggcc
    24661 ggcgcaggag gccctacttc gccagacgct cgcccaggcc ggtgtggtcc cgaacgagct
    24721 cagcttcgtc gaggcccacg gaaccggcac cgcgcttgga gatctgattg agctccgtgc
    24781 cctgaggacc gtcctgctcg agggccgcag cccccagcaa cggtgtgcga tcgcctcgat
    24841 caagagcaac atcggacata ccgaggcggc gtctggactc gcggggatga tacgggtcct
    24901 gctctccatg cagcatggag tcatcacacc caacctccac ctgcagaagc tgagccccca
    24961 catcgccacg gcgatcgacg gaacgccgct gttcattccc acccaggccg agccctggac
    25021 ggtcgcaggg agacgactgg ccgccgtcag ctcgttcagc ttcggtggcg ccaatgccca
    25081 ggtgatcctg gaacaggcac gcggcccggc ggcggcgccc gaccgctcga gcccgccgtc
    25141 gccacatgca ttgatgctct cggcgcggag caccgaaggc ctgcgggagc tggcgcggtc
    25201 gtacgcagcc tatctgaccg cgcatcccag cactgcgctg gtcgacgtct gcttcacggc
    25261 aaacaccgct cgctcccagt tcgatcacca gctgtgcgcc gtgtccacct ccgtggacag
    25321 gctcggcagg caactgagcg acttcgccgc gggtcgtccc gcagaagggc tgtgggccgg
    25381 gcagcgcagc ggcaggaagc gccccacgat cgtcctccag cttcgcggtg ccggccccac
    25441 accgcctcac acctcgatgc gccggctcgt cgagcgatgc gcaccgctgc tccctgcccc
    25501 cctgctttgc caggtgagct tgtccgcgga acaaggcgaa ccgctgagcc cttcggcgta
    25561 tcgggctgtg cacgttgccg tccaactcac cgtcgctcgc cagtggatgg cctggggcct
    25621 gcgccccacc gcggtgacgg gagacggcgt gggcgagatc gcggccgcat gtgtgcgtgg
    25681 ttcgatgacc accgaggagg catggcgata cgtcctgtcg gacggcgagt tgccgaggcc
    25741 ggtgccgccg gcgcccttcg agcgagccgg gaagttgatg tgcctggcga tctcgtcgga
    25801 cacggagacg ttgagcgtcg aggatccctc gggggaaccg cgcgtgctgg tgcggggcga
    25861 gctcatcgac ggcgaacggc agacgcactt ccactgcctg gcgaaccttg cggtgcatgg
    25921 cgcggacatc gactggcggg cggtgtactc ggagtttccg gcacagcgca tcgcactgcc
    25981 tacgtatcca ttccagcggc agcgctactg gatggcagcc ggatccgccg cgcccgctcc
    26041 agccggccgt gacagcggag gccgagccag caacctcttg tgatgcccaa ggccaagtaa
    26101 cccagacgcc ggagggggtc agttttagtg tcgcaacggg gtcagttctt cgtgtcgctt
    26161 gacagcagcc gcggcacgcc gatcgctacg tggcacgcac ctggcccgcg cgtcaccatc
    26221 gcgaccccgg ggccgaccgg agatggatcg gagattcgat tcccttgcgt ttccgccatc
    26281 ttgcaccacg cgttccaccc gatctacatc ggcgcgacac agtacctgat gtctcccatc
    26341 gccttcctcc acaggccgct gcgctggctc gcggccgtgt cgcgctatcg cgcgacgatc
    26401 agcggtgcgc ccaacttcgg ctacgagctg accgcgcggc gcgtgacccc gcagcaggcc
    26461 tcggggctcg acctgtcgag ctcgcaactg gtgttcaacg gcgccgggcc gatccgggtc
    26521 gagaccatgc ggcggttcgc cgccgcgttc gagcgctgcg gcttccggtg gtcgtcgttc
    26581 agcccctgct acgggctggc caaggcaacg ctggcggtca ccggctcggc aaggggccgg
    26641 gagcctcgcc tgtggcacgg tggaacgcgg cgagctggag gctcaccgcg tggtcgagct
    26701 gccgcccggt gccgccgatg cggtgacgct cgtcggatcc gagttccgcg accctcccga
    26761 gcaggttacg ggcgagatct cccgtgcgcg agtaggtggc gcgccgcgcg ggtgtcctcg
    26821 gcggcgatca ggtcggcctc gcgcagcacg cgcgccgcgc gcagcgacag gtcctccagg
    26881 ttgccgatcg gcgtgccgac gatgaacagg gtgccgatca cggtgctctc atccctacgc
    26941 cgggacggcg ccggtggcca tgaccggcac gcgtcgatcg cagcgtcggc gctgtccggg
    27001 ttttcgaggc tctgtccggg atccggccgg acaagcgccg gacaccggac agcctgcgga
    27061 gatcttccag cggttacgga tggcactgtt cgtgctggcc ttggcgggct ccaacctata
    27121 gaaagagagt agaccatgga caacgacgac aggagagagc gggcgatgga cggcgacgat
    27181 ctgaccgggc acgtgctggt cgcgatccgc cgggacatct ccgatctccg ggcggacatg
    27241 gacgagcggt tcgagcgcgt cgacgcgcgg atggatcgcc tcggcgaccg gctcgacagt
    27301 cctatcaccg agacgcagat gcggctgagc acccagatct tcgagctgca cggacgagtc
    27361 aacgacctgc agggcaccgt gcacgacatg cagggcaagc tcgacgacgt gcacggcatg
    27421 ctgcgcgacc tgcacgggcg gttcggcagg cggaagcctc gcaactgacc cgccaccgcc
    27481 ggtaggtgca gtgacggcgc accctcatgc gcgccgtcct cagtcgcggc tgagcgcgcc
    27541 gatgtcgacc gaggtgccga gctcggactc caggtacttc ttgagccggt ccagcatgcg
    27601 cttctcgagc tggcgggcgc gctcgcggct gacctggtag cggtcgccga tctcctgcag
    27661 cgtgagcggc tcgtcggtca gccagcgctc gcggaagatc gtctgctcgc ggccctccag
    27721 ggtctgggcg aactgctcga gcttgccgcg caacagctcg gagaactcgc tctgggcgac
    27781 cgcgcggtcg ggccggacgt cctcgctcgg caggtagtcg agccgggtgc gcgagccgtc
    27841 gtcgtcgttg ctcgtgccga gcggcgcatc gagcgaggcc tcgggggcgg cgagccggcg
    27901 ctccatctcg acgacctcct tctccgggac gtcgagcttc tcggcgagca gcgccgaggt
    27961 cggctggaac ccgagctgct cgagcttctc gcgctccttg cgcaggttga agaacagctt
    28021 gcgctgcgcc tgcgtggtgc cgatcttgac cagccgccag ttgttgagga tgaacttgag
    28081 gatgtacgcg cggatccaga acgcggcgta gctcgacagc ttgaccccgc ggtacggatc
    28141 gaacttcgac accgcctgga tcaggccgat gttgccctcc tgcaccaggt cgagcaggtt
    28201 cttgtgggcc cggcggtact cgtaggcgat cttgaccacc agccggaggt tggcctcgat
    28261 cagcttgcgc gccgcctggg tgtcgccgtg ctcgaccagc cgggtcgcca gctcgcgctc
    28321 ctcctcgggc gtgagcagcg gatagcgccg ggtctcgcgc aggtagaccg ccatcgggtc
    28381 gcgcttggcg agcgagctgc cgcggctctc ggccggcgcc gcctcggcat cgtcctccag
    28441 cacctcgtcg ggctcgatcg ccgccgcggc ggtggcgagg tcggtgtcgg cgccgcccga
    28501 ggccgcgtcg atctccgcct cgccgtcggt ctcgggctcg ccgtcggcgt cgtgcgcgc
//
LOCUS       DASVOR010000993         5616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_68263,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000993 DASVOR010000000
VERSION     DASVOR010000993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68263"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VF516_19260"
     CDS             complement(<1..721)
                     /locus_tag="VF516_19260"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8109882.1"
                     /translation="MTASRDGRIRSRWAPYVLTARYLALFALACFFLMPVYALLVTAL
                     KSPTEVSVVRMWQWPHALSIDNFRIVWPKLRDGFGNSVAMALPASVISSLLGAANGFV
                     LAKWRFPGADFVFALILFGMFIPYQAVLIPMFQTMSYTGLRGGLSGLVLVHVIYGLPI
                     TTLIFRSYFAGVSEELIEAAKVDGAGMLASFAFVALPIAPPAFAVAMIWQFTSAWNDF
                     LFGVLLTTKDRWPITVALNNIA"
     gene            complement(718..1611)
                     /locus_tag="VF516_19265"
     CDS             complement(718..1611)
                     /locus_tag="VF516_19265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8109883.1"
                     /translation="MMRRIRAWGPGLVLILPSLIALAIFVYGFLGWNVRVSLSSWRGL
                     SAAHDFVGLANYTALFDDLRFTRFDVPNIALFTAVFVAGSLALGFVLAALLDRGLPGE
                     QAFRSIYLFPMAISFIATAIIWRWLLDNGGGESTTGINKLLADAGLDWRSDWFKSESR
                     WAIAAIALPAGWSLSGYVMSLFLAGMRGVPDELREAARVDGASEPRVFWHVVRPMLLP
                     VLMSAVVILAHISLKTFDLFYAMDQKNLKIDTPALYMWFTTFDGGFYARGAAIAALLL
                     LGVATVIVPYIGYSVRAERTR"
     gene            complement(1637..2974)
                     /locus_tag="VF516_19270"
     CDS             complement(1637..2974)
                     /locus_tag="VF516_19270"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8109884.1"
                     /translation="MRRQLPSQIAVTLATAVMCVASMAACSKSKEPAKEPPKEAVKEP
                     AVKEPAKEPAKDQVEVFSWWTGAGEQEGLDAMIADFKAKNPGIEVINAAVAGGAGTNA
                     KAILANRLQAGNPPDSYQRHAGLELADDIKAGKVEDLTKLYEAQGWKDKLPRGLLDAI
                     TIDGKIYSVPVNIHRANLIWFTPKTLKALGIAGPPKTWKEFLTQAQAIKAKGKTALAI
                     GPAWTQKHLLETVLLGELGPDKYTGLWSGKTDWKSAEVTAALDTFKQVLAASDIKSAA
                     ADWQPAADRVIDGAAVYNVMGDWQDGYLGRAKKLTFQTDYDVAPSPGSAGVYDFLSDS
                     FTLPVGAKHHAAAEKWLIECGSTQGQDLFNPQKGSVPARTDADKSKYQGYLATALKDW
                     QDPATKIVGSLAHGVVANNAFGAEIDTALGIFVETRDAAGFAAAVAKAYAATK"
     gene            3286..>5616
                     /locus_tag="VF516_19275"
     CDS             3286..>5616
                     /locus_tag="VF516_19275"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEX8109885.1"
                     /translation="MIAFVLGGSACSNLPADRDPAEPALAGVEQAATLNLTQFVNPLI
                     GTDDSSSPNPVPGGAGGSTYPGPLLPFGMVQLSPDTPTASPSGYRNRDTSIEEFSLTH
                     FDGAGCPNNEDINLLPITGALGASPGTSWTSYASAYTKANESAAAGFYRTVLDRYATT
                     VELTATTRTGVFRVTYPSTTSAQLLINASRSATGSRSGSVTISGSTITGQVTAGGFCG
                     SSKTFPIFFAIQFDRAPTAHGTWSGGTISNGSSSVSGTSTGGFVTFDTTSNRVVQFAV
                     ALSYVSAANASQNLTAENPSLDFNARRTAADTAWNTMLNRIQATGGATADSQKFYTAL
                     YHAFSSPNVASDVNGQYRGFDNVVHTASGMTVYQNYSGWDIYRSWAALMGLVAPDIMT
                     DIVKSMVLDGQQGGLLPKWSQESAENFVMTGDPGPIIVASAFAFGVKNFDTTTALSLM
                     DKSSNSGTTQGSPIRGRESGYLSKHFIVDDPSDSLEYSASDFAISQFAAALGNTSLAS
                     TYMARAQFWRNVWDPESSYINRHNADGTVLAGLDPASSTGYTEGNAAQYTWMVTYDFA
                     GLIDLMGGPQTASQRLDHHFTQVNGGLTTPFFYIGNEPEHGVPWAYNFARNPAGTARA
                     VHKVMTDGFNTTAGGLPGNDDLGATSAWFVWAALGMYPATPGADTLALHGPSFPSILI
                     QRTAGNVTISASGVSTTNQFVQGVTVNGAATTHSFIRYPDIAAGGSLTYTMAASPSSW
                     GTGAGDVPPSFTAGALPTVPAPALGTNLALGRAVTAS"
BASE COUNT          901 a         2025 c         1870 g          820 t
ORIGIN      
        1 cggcgatgtt gttgagcgcg accgtgatcg gccagcggtc cttggtggtc agcagcacgc
       61 cgaacaggaa gtcgttccac gccgaggtga actgccagat catcgcgacc gcgaacgccg
      121 gcggcgcgat cggcagcgcg acgaacgcga agctcgccag catgccggcg ccgtcgacct
      181 tggcggcctc gatcagctcc tcggacaccc cggcgaagta gctccggaag atcagcgtgg
      241 tgatcggcag gccgtagatc acgtggacca gcaccagccc ggacaggccg ccgcgcagcc
      301 cggtgtacga catggtctgg aacatcggga tcagcaccgc ctggtacggg atgaacatcc
      361 cgaacaggat cagcgcgaac acgaagtcgg cgccggggaa ccgccacttc gccagcacga
      421 agccgttggc ggcgccgagc agcgacgaga tcacgctcgc cggcagcgcc atcgccacgc
      481 tgttgccgaa cccgtcgcgc agcttgggcc acacgatccg gaagttgtcg atcgacagcg
      541 cgtgcggcca ctgccacatc cgcaccacgc tgacctcggt ggggctcttg agcgcggtga
      601 ccagcagcgc gtacaccggc atcaggaaga agcaggcgag cgcgaacagc gccaggtagc
      661 gcgcggtcag gacgtacggc gcccaccgcg accggatccg gccgtcgcgc gaggcggtca
      721 ccgcgtgcgc tccgcgcgca cggagtaccc gatgtacggc acgatcaccg tcgcgacacc
      781 gagcaggagc agcgcggcga tcgcggcgcc ccgcgcgtag aacccgccgt cgaacgtggt
      841 gaaccacatg tagagcgccg gcgtgtcgat cttgaggttc ttctggtcca tcgcgtagaa
      901 caggtcgaac gtcttgagcg agatgtgcgc gaggatgacc accgcgctca tcagcaccgg
      961 caacagcatc ggccggacga cgtgccagaa cacgcgcggc tcgctggcgc cgtcgacccg
     1021 cgcggcctcg cgcagctcgt cgggcacgcc gcgcatgccg gcgaggaaca gcgacatcac
     1081 gtagcccgac agcgaccagc cggcgggcag ggcgatcgcg gcgatcgccc accgcgactc
     1141 ggacttgaac cagtcgctgc gccagtcgag gcccgcgtcg gccagcagct tgttgatgcc
     1201 ggtggtcgac tcgccgccgc cgttgtcgag cagccagcgc cagatgatcg cggtggcgat
     1261 gaacgagatc gccatcggga acaggtagat gctgcggaac gcctgctcgc cgggcaggcc
     1321 gcggtcgagc agcgccgcca gcacgaaccc gagcgccagc gagccggcga cgaacaccgc
     1381 ggtgaacagc gcgatgttgg gcacgtcgaa ccgggtgaac cgcaggtcgt cgaacagcgc
     1441 ggtgtagttg gccagcccga cgaagtcgtg cgccgcggac aggccgcgcc agctcgacag
     1501 cgagacccgg acgttccagc ccaggaagcc gtagacgaag atcgcgagcg cgatcagcga
     1561 gggcaggatc aacaccagcc ccggtcccca ggcgcggatc cgtcgcatca tgatggggcg
     1621 tcggtccggc ggcgcgctac ttcgtggcgg cgtacgcctt ggcgaccgcg gccgcgaacc
     1681 ccgcggcgtc gcgggtctcg acgaagatgc cgagcgcggt gtcgatctcg gcgccgaacg
     1741 cgttgttggc gaccacgccg tgcgcgagcg agccgacgat cttggtggcg ggatcctgcc
     1801 agtccttgag cgcggtcgcg aggtagccct ggtacttgga cttgtcggcg tcggtccgcg
     1861 ccggcaccga gcccttctgc gggttgaaca ggtcctggcc ctgcgtggag ccgcactcga
     1921 tcagccactt ctcggcggcg gcgtggtgct tggcgccgac cggcagggtg aacgagtcgg
     1981 acaaaaagtc gtacacgccg gccgagccgg gcgacggggc gacgtcgtag tcggtctgga
     2041 acgtcagctt cttggcccgg ccgaggtagc cgtcttgcca gtcgcccatc acgttgtaga
     2101 ccgcggcgcc gtcgatcacg cggtccgccg ccggctgcca gtcggctgcc gccgacttga
     2161 tgtccgaggc cgcgagcacc tgcttgaacg tgtcgagcgc cgccgtcacc tcggcggact
     2221 tccagtcggt cttgccgctc cacagcccgg tgtacttgtc ggggccgagc tcgccgagca
     2281 gcacggtctc gagcaggtgc ttctgggtcc acgccgggcc gatcgcgagc gcggtcttgc
     2341 ccttggcctt gatcgcctgg gcctgggtca agaactcctt ccaggtcttc ggcggcccgg
     2401 cgatgccgag cgccttgagg gtcttgggcg tgaaccagat caggttggcg cggtggatgt
     2461 tgaccggcac cgagtagatc ttgccgtcga tcgtgatcgc gtcgagcagg cccctgggca
     2521 gcttgtcctt ccagccctgc gcctcgtaca gcttggtcag gtcctcgacc ttgccggcct
     2581 tgatgtcgtc ggcgagctcc agcccggcgt ggcgctggta cgagtccggc gggttgccgg
     2641 cctgcagccg gttggcgagg atcgccttgg cgttggtgcc cgcgccgccg gcgaccgcgg
     2701 cgttgatgac ctcgatcccc gggttcttgg ccttgaagtc ggcgatcatc gcgtcgaggc
     2761 cctcctgctc gccggcgccg gtccaccacg agaacacctc gacctggtcc ttggcgggct
     2821 ccttcgcggg ctccttgacg gcgggctcct tcaccgcctc cttgggcggt tccttggcgg
     2881 gctccttgga cttggagcac gcggccatgc tggccacgca catcacggcg gtcgcgagtg
     2941 tcacggcgat ctggctcggc agctgtcttc gcatggagca acctcctaca ggcgcacacg
     3001 atgcgcccgg cggtgcagtc gtgtcaattc catcgctgga ccggcgcgga atcggaccga
     3061 ccgcacgggt ggcggcgcac gccgtgcgcg gcgcgtgatg acaacgttgt catggtgtca
     3121 tgacgcttac gcgtcgcgcg gcgatcgacg agttcgcttg ctacgcacac gagcgacgat
     3181 tcaagatccg cccccctgac tggcgcatgc ccggtgctcg ggcgcatgcg cgacgccaac
     3241 ccccgcctcg agaggtgacc tgtgcgtgtg cttcgatcca aagcggtgat cgcgttcgtc
     3301 ctgggcggca gcgcctgcag caatcttccg gccgatcgcg acccggccga gcccgcgctc
     3361 gccggcgtgg agcaggccgc gacgctcaac ctgacccagt tcgtcaatcc gttgatcggc
     3421 accgacgaca gcagctctcc caacccggtg ccgggcggcg ccggcggcag cacttacccc
     3481 ggaccgctcc tgccgttcgg catggtccag ctcagcccgg acacgccgac cgcgtcgccc
     3541 tcgggctacc gcaaccgcga cacctcgatc gaggagttca gcctgaccca cttcgacggc
     3601 gcgggctgcc ccaacaacga ggacatcaac ctcctgccga tcaccggcgc gctcggcgcg
     3661 tcgcccggca cgtcgtggac cagctacgcc tcggcctaca ccaaggccaa cgagtccgcg
     3721 gccgccgggt tctaccgcac cgtgctcgac aggtacgcga ccacggtcga gctgaccgcg
     3781 acgacccgca ccggcgtgtt ccgcgtgacc tacccgtcga cgacctccgc ccagctcctg
     3841 atcaacgcca gccgcagcgc gaccggcagc cgcagcggct cggtgacgat ctcgggctcg
     3901 acgatcaccg gccaggtcac cgccggcggg ttctgcggct cgtcgaagac cttcccgatc
     3961 ttcttcgcga tccagttcga ccgcgccccg accgcgcacg gcacgtggtc gggcggcacg
     4021 atctcgaacg gctcgagctc ggtcagcggg accagcaccg gcgggttcgt cacgttcgac
     4081 accacctcca accgggtcgt ccagttcgcg gtcgcgctgt cctacgtcag cgcggccaac
     4141 gcgagccaga acctgaccgc cgagaacccg agcctggact tcaacgcgcg ccgcaccgcc
     4201 gcggacaccg cgtggaacac gatgctcaac cggatccagg ccaccggcgg cgcgaccgcc
     4261 gattcccaga agttctacac cgcgctgtac cacgcgttct ccagccccaa cgtcgcgagc
     4321 gacgtcaacg gccagtaccg cgggttcgac aacgttgtcc ataccgccag cgggatgacc
     4381 gtgtaccaga actactcggg ctgggacatc taccgctcgt gggcggcgct gatggggctg
     4441 gtcgcacccg acatcatgac cgacatcgtc aagtcgatgg tgctcgacgg ccagcagggc
     4501 ggcctgctgc ccaagtggtc gcaggagagc gccgagaact tcgtgatgac cggcgacccg
     4561 gggccgatca tcgtcgcgtc ggcgttcgcg ttcggcgtca agaacttcga taccaccacc
     4621 gcgctgtcgc tgatggacaa gagctccaac agcggaacca cccagggcag cccgatccgc
     4681 ggccgcgagt ccggctacct gtcgaagcac ttcatcgtcg atgatccgtc ggactcgctc
     4741 gagtactcgg cgtcggactt cgcgatctcg cagttcgccg ccgcgctcgg caacacgtcg
     4801 ctggcatcga cctacatggc gcgcgcgcag ttctggcgca acgtgtggga ccccgagtcg
     4861 tcgtacatca accggcacaa cgccgacggc accgtcctgg cggggctcga tccggcgagc
     4921 agcaccggct acaccgaggg caacgccgcg cagtacacct ggatggtcac ctacgacttc
     4981 gccggcctga tcgacctgat gggcgggccg cagaccgcca gccagcggct cgaccaccac
     5041 ttcacccagg tcaacggcgg gctgaccacc ccgttcttct acatcggcaa cgagcccgag
     5101 cacggcgtgc cgtgggccta caacttcgcg cgcaaccccg ccggcacggc gcgcgcggtg
     5161 cacaaggtca tgaccgacgg gttcaacacc accgcgggcg ggctgcccgg caacgacgac
     5221 ctcggcgcga cgtcggcgtg gttcgtgtgg gccgcgctcg gcatgtaccc ggcgaccccg
     5281 ggcgccgata cgctcgcgct gcacggcccg tcgttcccgt cgatcctgat ccagcgcacc
     5341 gccggcaacg tcacgatctc ggcgagcggc gtcagcacga ccaaccagtt cgtccagggc
     5401 gtgaccgtca acggcgccgc gaccactcac agcttcatcc ggtacccgga catcgcggcc
     5461 ggcggctcgc tgacctacac catggccgcc agcccgagca gctggggcac gggcgccggc
     5521 gacgtgccgc cgagcttcac cgcgggcgcg ctgccgacgg tcccggcccc ggcgctcggt
     5581 accaacctcg cgctcggccg cgcggtcacc gcgtcc
//
LOCUS       DASVOR010000994         2375 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_243852,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000994 DASVOR010000000
VERSION     DASVOR010000994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243852"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="VF516_19280"
     CDS             <1..1067
                     /locus_tag="VF516_19280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109886.1"
                     /translation="LRAGLPLGEDRGLEGASIVMTVLGTYGVLSTGGIVVMLGALSPH
                     HLRHGWGVMLLVVFALLVVRSSLHIRAGLAGLRDSSAQLSFDRPSELAGRYAAFGVIS
                     AFCVGGVLSLLAMSERLTPDAILSVGVACWLLITWPMIVKRYFTQRQFAELLAGDRVT
                     HRRAPDTGLTALGWLLAGHATLIAGVVIVQVTGYPRLGPQLGRAADHALVLSGLAVGR
                     WWLIDASLAAASVVLELAAAVALIRMSDQRRAITTIYAVFAGVVALAPAVPVLASLGH
                     HLSIWTGVQLLPSAVQLVMPVAALVLVHRVVMPAARARYRYRSHGGPPPPTPPAKPSG
                     SPPPVSLRSTELSGIDVIEA"
     gene            1080..>2375
                     /locus_tag="VF516_19285"
     CDS             1080..>2375
                     /locus_tag="VF516_19285"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8109887.1"
                     /translation="MSEGSPTERFGPYIVYEQLGIGGMAQVHRAVAADSDVQRPVALK
                     RMLSHIASNQDMVKSFVREARLASYLRHENVAQTYDLGRVGDTYFIVMELITGRNLRE
                     VLRHCGATTGPMPVPLALNILNQICDALDYAHNLCDETGTPLGIIHRDVSPSNVIVST
                     TGMVKLIDFGIAKASAAGMQTMSGTLKGKFAYMAPEYLAGRIDPRADLFAVGVIAHEL
                     LTNRPLFSGRDDIDTLTRVRDMHVAPPSHENPLVPSEIDDVVMTALARDPERRWQHAT
                     ALRSALTTLTRRLGLVASNAQVVEWLDWAFTQKRPARPRGEPDTETDGEPLADEPSIS
                     IQQPTVDLSPKSQQITLRRNDGPRDAARGPLSIDDAPEPPPKAPVGTPLYVPAQHPLP
                     AGPTGHTDRSGPVAAPIARASASIPVRAPSGTAPPTGRLP"
BASE COUNT          304 a          917 c          816 g          338 t
ORIGIN      
        1 cgctgcgcgc ggggctgccg ctcggcgagg accgcggcct ggagggcgcg tcgatcgtca
       61 tgaccgtgct cggcacgtac ggcgtgctgt cgaccggcgg gatcgtcgtg atgctcggcg
      121 cgctgtcgcc gcaccacctg cgccacggct ggggcgtgat gctgctcgtg gtgttcgcgc
      181 tgctcgtggt gcgctcgtcg ctccacatcc gcgccgggct cgccggcctg cgcgacagct
      241 cggcacagct ctcgttcgac cgcccgagcg agctcgccgg gcgctacgcc gcgttcggcg
      301 tgatcagcgc gttctgcgtc ggcggtgtgc tgtcgctgct cgcgatgtcg gagcggctga
      361 ccccggacgc gatcctcagc gtcggggtgg cgtgctggct gctcatcacc tggccgatga
      421 tcgtcaagcg ctacttcacc cagcgccagt tcgccgagct gctcgccggc gaccgcgtga
      481 cccaccgccg cgcccccgac accgggctga ccgcgctggg ctggctgctc gccggccacg
      541 ccaccctgat cgcgggggtc gtgatcgtgc aggtcacggg ctatccccgg ctcggcccgc
      601 agctcggtcg cgccgccgac cacgcgctcg tgctcagcgg gctggcggtc ggccggtggt
      661 ggctgatcga cgccagcctc gcggcggcgt cggtcgtgct cgagctcgcc gccgcggtgg
      721 cgctgatccg gatgagcgac cagcgccgcg cgatcaccac gatctacgcg gtgttcgccg
      781 gggtggtcgc gctcgccccg gcggtcccgg tgctcgcctc cctgggccac cacctgagca
      841 tctggaccgg ggtccagctc ctgccgtcgg cggtccagct cgtgatgccg gtcgcggcgc
      901 tggtgctggt ccaccgcgtg gtcatgccgg cggcccgcgc ccgctaccgc tatcgatccc
      961 acggggggcc tccgcccccc accccgccgg cgaagccgtc ggggtcccca ccccctgtct
     1021 cgctgcgctc gacggaactt tccggtatcg acgtgatcga agcctgatac catgccaggg
     1081 tgagcgaggg ctcgccgacc gagcgattcg ggccgtacat cgtgtacgag cagctcggga
     1141 tcggtggtat ggcgcaggtc catcgcgccg tggcggcgga ctccgacgtc cagcgcccgg
     1201 tggcactcaa gcgcatgctg tcgcacatcg cgagcaacca ggacatggtc aagtcgttcg
     1261 tccgcgaggc ccggctcgcg agctacctgc gccacgagaa cgtcgcgcag acctacgacc
     1321 tcggccgggt cggcgacacc tacttcatcg tgatggagct gatcaccggg cgcaacctgc
     1381 gcgaggtgct ccgccactgc ggcgccacca ccggcccgat gccggtcccg ctcgcgctca
     1441 acatcctgaa tcagatctgc gacgcgctcg actatgccca caacctgtgc gacgagaccg
     1501 gcacgccgct cggcatcatc caccgcgacg tgtcgccgtc caacgtcatc gtgtcgacca
     1561 ccggcatggt caagctgatc gacttcggga tcgcgaaggc gtcggccgcc ggcatgcaga
     1621 cgatgagcgg cacgctcaag ggcaagttcg cgtacatggc gcccgagtac ctcgcggggc
     1681 ggatcgaccc gcgcgccgac ctgttcgcgg tcggcgtgat cgcgcacgag ctgctcacca
     1741 accggccgct gttctcgggc cgcgacgaca tcgacacgct gacccgcgtg cgcgacatgc
     1801 acgtcgcgcc gccgtcgcac gagaacccgc tggtgccgtc cgagatcgac gacgtcgtga
     1861 tgaccgcgct ggcgcgcgac cccgagcgcc gctggcagca cgccaccgcg ctgcgcagcg
     1921 cgctgaccac gctgacccgc cggctcggcc tggtcgcgag caacgcccag gtcgtcgagt
     1981 ggctcgactg ggcgttcacc cagaagcgtc cggcgcggcc gcgcggcgag cccgacaccg
     2041 agaccgatgg cgagccgctc gccgacgagc cgagcatctc gatccagcag ccgacggtcg
     2101 acctgtcgcc gaagagccag cagatcacgc tgcggaggaa cgatggcccg cgcgacgcgg
     2161 cgcgcggccc cctctcgatc gacgacgcgc ccgagccgcc gcccaaggcg ccggtcggca
     2221 cgccgctcta cgtcccggcg cagcacccgc tgcccgccgg gcccaccggc cacaccgatc
     2281 gctccggccc cgtggcggcg cccatcgcgc gggcctcggc gtcgatcccg gtgcgcgcgc
     2341 cgtccggaac cgcgccgccc accggccggc tgccg
//
LOCUS       DASVOR010000995        12077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_126779,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000995 DASVOR010000000
VERSION     DASVOR010000995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126779"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VF516_19290"
     CDS             <1..975
                     /locus_tag="VF516_19290"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEX8109888.1"
                     /translation="PSRAMASRKSIQPVVDARGRLLLERRGLPRGVAESLRKDAYHFL
                     RTTTWTRLTLLFAGLFLAVNLVFAAILYLGGAEVMNAHGFLDDFWFSVQTLATIGYGY
                     LAPADALANAVVTVESFVGISLTAVITGVFFARFATPYAKVIFSRVAIIGEHDGKRTL
                     MFRMANARSTAILEATCRVYVTRDEILAGGERMRRVHDLPLRRSTSPIFSMSWLATHT
                     IDAASPIADLTPETLAAANCNIIVTFQGIDDRLAATVHTRYAYNAEDIVFGHRFADIF
                     RTDPGTGQRYLDFEVFHATEPVLPASAAVVEVAEPVEAAAASAGADPA"
     gene            complement(950..2782)
                     /gene="aspS"
                     /locus_tag="VF516_19295"
     CDS             complement(950..2782)
                     /gene="aspS"
                     /locus_tag="VF516_19295"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEX8109889.1"
                     /translation="MSSFLANHTRTHSCGELRAADAGKHVVLTGWVQTYRDHGERIFI
                     DLRDRDGVTQVVADRTRLPAVHELANALRAEWCIGIAGEVRMRGEQLAKADPGEERKL
                     KKMTNEKLPTGEIEVWIDELEVFSRSETPPFAIEDDLDTNDQLRFKYRYLDLRRPRMQ
                     QNLMTRSKITRTTRDFLGKNGFLEIETPFMVKYTPGGARNFLVPSRLNPGSFYALAES
                     PQIFKQLLMVGGYERYFQIVRCFRDEDLRLDRQPEFTQIDIEMSFVNERILQTTMEGL
                     IAALWKQVLGIELALPLRRMTFAEAMDKSGVDKPDLRLDLTLCDVTQGFQASGFKIFE
                     TVIAGGGIVKCLRIPKNDKLTRAIIDGLPDFGKQFGLKGVTTVRLQDGGAWQGLKGVS
                     DEARQRVNQIAGAEVGDALVMVADKPKLANTALGGIRLHLGDKLGLTRKGEWQFMWLT
                     DPPLFEVDDATREIAAAHHPFTSPRVEDEPLLETAPERVLARAYDLVLNGVEIGGGSI
                     RIHRSDLQARVFQALQIGPDEQRAKFGFLLDAFKFGPPPHGGIAFGLDRLAMLMAGAE
                     SLRDVIAFPKTQKGSDLMTECPTPVSRAQLDELFIQVRPDLPQR"
     gene            3042..4034
                     /locus_tag="VF516_19300"
     CDS             3042..4034
                     /locus_tag="VF516_19300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109890.1"
                     /translation="MSEALPASFAPFVEPCAGPSLRSETASASRAAALVEALRRAKKT
                     AAAVPSEVTDAQDSLERRIRAHLTHGRVQVTLTDNRYTMISVRRIGARDDRGGTGGPA
                     GSAGGAGGKAPRGIERSEMGGGGPAGPAGGAGGKAPRGIERRYEVRLHHMFADADPVI
                     TRALAHYIADNDADASRILGDFIDANSGHVRGRSRRAPSQLILTAGEHHDLREIFDEL
                     NARYFDHRIDAAITWGPRSGRPRRRNSIKMGSYSVEDRLIRIHRSLDRAFVPRFFVAW
                     IVFHEMLHQVHDIRVKNGRREFHSKEFLAAEAGFELYEQAKLWERRHLDALLTY"
     gene            4191..4865
                     /locus_tag="VF516_19305"
     CDS             4191..4865
                     /locus_tag="VF516_19305"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8109891.1"
                     /translation="MEERMGESIVLGERRRALRVPVRGFAVLYASTGPLHAMIENLSK
                     SGALVSVASRLPEEQLDIEIRIAEGNGWVVGRVVRAEPHARQWRIAVEFGRVDDSLKV
                     AIEASIASARGAARRRPILVIDENTDRKNSLITRLSDRGMTPLAPKTPLEAIDLLTRS
                     QLHVGVCLLAPGFGVPSHDLRAVLSDSFPWVSVAEITDDLDATTHHAIDTWNTTPIGR
                     IADAMA"
     gene            complement(4875..5156)
                     /locus_tag="VF516_19310"
     CDS             complement(4875..5156)
                     /locus_tag="VF516_19310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109892.1"
                     /translation="MQRELLARGAVTALVTNCGCLGPCFDGPNAVVYPDGVWYAGLQP
                     DDAAGIADHLVAGRVLAAKVSQRPGAEPASDPVTAGEAVGEKDGYAGRR"
     gene            5423..6112
                     /locus_tag="VF516_19315"
     CDS             5423..6112
                     /locus_tag="VF516_19315"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pvc16 family protein"
                     /protein_id="HEX8109893.1"
                     /translation="MTPQHHIIKETSETLSRLLQDEFKRNGYKRVHIVEQAPKPDAIE
                     GKLPAVSVYLYQMSIDPEGTDSTPHSELVEVPQEDGTIKEYLRRRRLWVRLDYLMSAW
                     AQTPEDEQLLLGLIIRTIMENPELPREKLKGQSFEDDFKLNILMSARLDEGTLARFWG
                     SLNQPVRPAIQAWTAIPILPEKLEEFKRVQSRRLEYKSITDPQISEVGPSGNPFLAAR
                     RLDLGGGTGKK"
     gene            6210..7430
                     /locus_tag="VF516_19320"
     CDS             6210..7430
                     /locus_tag="VF516_19320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HEX8109894.1"
                     /translation="MATSYLSPGVYVEEVDRGSKPIEAVGTNTVGFLGESAKGPLNES
                     VLITNWSQFVRTFGDFKDCSEHFAHAVYGFFNNGGSRCFVVNVGAPEGAKVETKPVAP
                     AGDKKEDKAPAAVHVGGGGRDGLYIGKDGGPGARTGLKCFDEIDEIAIVCAPGQTSPA
                     VQDALLSHCETRKDRFAILDSPETISGGVDKLPKPRDSKYGAYYFPWIQVYDPDKGNI
                     FVPPAGHIAGVYSRTDSERGVHKAPANEIVRGALGLKYNVSKGEQDLLNPKGINAIRF
                     MNGGIRIWGARTLSSDPSWRYINVRRLFIMVESSIERATQWVVFEPNDHRLWKRVQRT
                     ISSFLTLLWRNGALMGTSPEQAFYVKCDDETNPPEVIDAGQLVVEIGLAPVKPAEFVI
                     FRIGQMASGGGVEE"
     gene            7743..8651
                     /locus_tag="VF516_19325"
     CDS             7743..8651
                     /locus_tag="VF516_19325"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8109895.1"
                     /translation="MADQRSYTGGNFRLDVGGIDVGYLRKVSGGGMKADIAEHKLGPD
                     NVIKKNVANIGWEEVKCEMGVGMGKAAYDWIKSAFAKQFVTQQATLVAGDFNHKAVQE
                     MQYIDCLITSVATPKFDGSDKNPAYFTVGFKPERVRHVPGDGKDIRAKMGPAQKAHLC
                     SNFRLELGDLPCNRVASVDAMELKCSVVKDDVGITRESTIHPAAVSVPDLKVTWSYAD
                     FTAVEKEARKWFVDGLHLEGNEMTGRIVLLDPNMKDEIGEITLKGVGWKEFKSDDREG
                     ASEKILRCTGTFYVEEMGISIKRYDA"
     gene            8769..9176
                     /locus_tag="VF516_19330"
     CDS             8769..9176
                     /locus_tag="VF516_19330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HEX8109896.1"
                     /translation="MAFKTQYAFRLPRGYVDEQGNVHRDGIMRLATARDEILPLQDYR
                     VQSNRAYLVIVLLSRVITKLGDHTHVAVDMIENLFSTDLAYLQEFYRKINEEGGAPKH
                     HITCPKCQNEMDVDMVTGALLTEGGGSGQSGPG"
     gene            9173..9355
                     /locus_tag="VF516_19335"
     CDS             9173..9355
                     /locus_tag="VF516_19335"
                     /inference="COORDINATES: protein motif:HMM:NF042798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6760 family protein"
                     /protein_id="HEX8109897.1"
                     /translation="MTWAAVATYPREQLYQEIAYIAYHFHWSLDDILDMEHEERHVWL
                     REIARVNREINEARRR"
     gene            9386..11146
                     /locus_tag="VF516_19340"
     CDS             9386..11146
                     /locus_tag="VF516_19340"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX8109898.1"
                     /translation="MRPSADQRAPGIYQTYDPVVIPPMSIANTRIAGFVGITHKGPMN
                     EPTRLGNWDEFLEIFGYSQDSYTSDSVYGFFKNGGTDCWVVRVAHNPPRGEIAGMEQA
                     ASAEHVQLDDWNKPTLKICALNEGSWGNAIWVRCVHAPAATALLISDLDIGAGEAQVN
                     TTRGFEVGSTVRIYDRENSDFIVITEVGDKRIKWSTDTPINRRHRAAAPTHLEVLTFE
                     IHVAMKDRREVFKGLQMHPSSRTYAPRLVSQRSRLIRLEDLRSKSPVPHNVPGVLPMT
                     RLTGGRDGLDAITPEDFIGLDNGPSERSGLLALAAIDEVALLACPDAMRFYDREPGPA
                     GELKAQRVQDQMISVCELQKDRFAVLDIPQSKDIEWVRRWRRRTDSSYCAYYWPWLRA
                     ESVDEKPRVLPPSGYLVGCYARRDLDGVHHAPANLEIEGVQDIALRVTEDHIGQLNAD
                     SVNTFRIQRGVRPWGARTASSDPEWRYIPIRRLFIMLRRSLEAGFAWITFEPNNHVTW
                     EIVRRRVTEFLAGLHQQGMLAGGNLEEAFFVKCDAETNPADQVDNGMLLCDIGVAPVH
                     PAEFIMISLTQTMNTPTSGS"
     gene            11159..11614
                     /locus_tag="VF516_19345"
     CDS             11159..11614
                     /locus_tag="VF516_19345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8109899.1"
                     /translation="MATPTTTRKDPLPVFCFLVRFTNLPGNVEGFFKSVSGLKFETEV
                     VPVSSGGANDTTFQLPGATKWGNLVFKQGFTNSSALMTWRQKWLQGQMTRTGGSIIQL
                     DTSLKPAAQWDFVRGWPCKWEIGEFDASKSELAIETLEIAHEGLTFSAA"
     gene            11683..>12077
                     /locus_tag="VF516_19350"
     CDS             11683..>12077
                     /locus_tag="VF516_19350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109900.1"
                     /translation="MANETEPRALSAALAAPAFVTRGHGAAMHAADRHIERWAPEAGA
                     GRVRSMRALGFVDRLVAPWVETAQRSASLRLFHQYASTGLGERPGGAVSWVFPRPWYQ
                     DELDWMASARRAGAQAGPAQTPSLLTTRGT"
BASE COUNT         2030 a         4113 c         4100 g         1834 t
ORIGIN      
        1 ccctcgcgcg ccatggccag ccgcaagtcc atccagccgg tcgtcgacgc gcgcggccgc
       61 ctgctgctcg agcgccgcgg cctgccgcgc ggcgtcgccg agtcgctccg caaggacgcc
      121 taccacttcc tgcgcacgac gacctggacc cggctgacgc tgctgttcgc cgggctgttc
      181 ctcgcggtca acctggtgtt cgccgcgatc ctgtacctcg gcggcgccga ggtgatgaac
      241 gcgcacggct tcctcgacga cttctggttc agcgtccaga ccctcgcgac gatcggctac
      301 ggctacctcg cgcccgccga cgcgctggcc aacgcggtgg tcaccgtcga gagcttcgtc
      361 gggatctcgc tgaccgcggt gatcaccggc gtgttcttcg cccggttcgc cacgccgtac
      421 gccaaggtga tcttctcgcg ggtcgcgatc atcggcgagc acgacggcaa gcgcacgctg
      481 atgttccgga tggcgaacgc gcggtcgacc gcgatcctcg aggcgacctg ccgggtctac
      541 gtcacccgcg acgagatcct cgccggcggc gagcggatgc gccgggtcca cgatctgccg
      601 ctgcgccggt cgacctcgcc gatcttcagc atgtcgtggc tggcgaccca cacgatcgac
      661 gcggcgagcc cgatcgccga tctcacgccc gagacgctcg ccgccgccaa ctgcaacatc
      721 atcgtcacgt tccagggcat cgacgaccgg ctcgccgcga cggtccacac ccgctacgcc
      781 tacaacgccg aggacatcgt gttcggccac cggttcgccg acatcttccg caccgacccc
      841 gggaccgggc agcgctacct cgacttcgag gtgttccacg ccaccgagcc ggtgctgccg
      901 gccagcgccg cggtcgtgga ggtcgccgag cccgtcgaag ctgccgcggc tagcgctggg
      961 gcagatccgg cctgacctgg atgaacagct cgtcgagctg cgcgcgggac accggggtcg
     1021 ggcactcggt catcaggtcc gagcccttct gggtcttggg gaacgcgatc acgtcgcgca
     1081 gcgactcggc gccggccatc agcatcgcca gccggtcgag gccgaacgcg atcccgccgt
     1141 gcggtggcgg cccgaacttg aacgcgtcga gcaggaaccc gaacttggcg cgctgctcgt
     1201 cggggccgat ctgcagggcc tggaacaccc gggcctggag atcgctgcgg tggatacgga
     1261 tcgagccgcc gccgatctcg acgccgttga gcacgaggtc gtaggcgcgc gccagcacgc
     1321 gctcgggtgc ggtctcgagc aggggctcgt cctcgacccg cggcgaggtg aacgggtggt
     1381 gcgcggccgc gatctcccgg gtcgcgtcgt cgacctcgaa cagcggcggg tcggtgagcc
     1441 acatgaactg ccactcgccc ttgcgggtca ggccgagctt gtcgccgagg tggagccgga
     1501 tcccgccgag cgcggtgttg gcgagcttgg gcttgtcggc gaccatgacc agcgcgtcgc
     1561 cgacctcggc gcccgcgatc tggttgaccc gctggcgcgc ctcgtcggac acgcccttga
     1621 ggccctgcca cgcgccgccg tcctgcagcc gcaccgtggt cacgcccttg aggccgaact
     1681 gcttgccgaa gtcgggcagc ccgtcgatga tcgcgcgggt cagcttgtcg ttcttcggga
     1741 tgcgcaggca cttcacgatc ccgccgccgg cgatcacggt ctcgaagatc ttgaacccgc
     1801 tggcctggaa gccctgggtg acgtcgcaca gcgtgaggtc gagccggagg tcgggcttgt
     1861 cgacgccgga cttgtccatc gcctcggcga acgtcatccg gcgcagcggc agggcgagct
     1921 cgatgccgag cacctgcttc cacagcgccg cgatcaggcc ctccatcgtg gtctgcagga
     1981 tgcgctcgtt gacgaacgac atctcgatgt cgatctgggt gaactcgggc tggcggtcga
     2041 gccggaggtc ctcgtcgcgg aagcaccgca cgatctggaa gtagcgctcg tagccgccga
     2101 ccatcagcag ctgcttgaag atctggggcg actcggcgag cgcgtagaag ctgccggggt
     2161 tgagccgcga cggcaccagg aagttgcgcg cgccgcccgg cgtgtacttg accatgaacg
     2221 gggtctcgat ctcgaggaag ccgttcttgc cgaggaagtc gcgggtggtc cgcgtgatct
     2281 tcgagcgggt catcaggttc tgctgcatgc gcggccggcg caggtcgagg tagcggtact
     2341 tgaaccgcag ctggtcgttg gtgtcgaggt cgtcctcgat cgcgaacggc ggggtctccg
     2401 accgcgagaa cacctcgagc tcgtcgatcc agacctcgat ctcgccggtc gggagcttct
     2461 cgttggtcat cttcttgagc ttgcgctcct cgccggggtc ggccttggcg agctgctcgc
     2521 cgcgcatccg gacctcgccg gcgatgccga tgcaccactc cgcgcgcagg gcgttggcga
     2581 gctcgtggac ggcgggcagc cgggtccggt cggccacgac ctgggtcacg ccgtcgcggt
     2641 cgcgcaggtc gatgaagatc cgctcgccgt ggtcgcggta ggtctggacc cagccggtca
     2701 ggacgacgtg cttgccggcg tcggcggcgc gcagctcgcc gcagctgtgg gtccgcgtgt
     2761 ggttggccag aaacgagctc atgggaccgg caccatagcg tatgcccgta ggacccggtg
     2821 cgatcccgcg atccaagtga ttttcccaag gcttgcgatc ggatccgatc ccttcgacgg
     2881 tgggaaattt gatccgcgcg gaagcgccgc gttagcattt tttccaggtc ggcgggagcg
     2941 agtttctcga cggaccggcg ggcctggtcc tgtcgggtga cgtgcgcgtc acgcagggaa
     3001 gctcgcgact tggccatgtg cttcaatatg aggagttcgc gttgagtgaa gctttgcccg
     3061 cctcctttgc gccgttcgtc gagccgtgcg ccggtccctc gctgcgaagc gagaccgcga
     3121 gcgccagccg cgcggccgcg ctggtcgagg cgctgcgccg ggcgaagaag accgcggccg
     3181 cggtcccgag cgaggtcacc gacgcccagg acagcctgga gcgccggatc cgcgcccacc
     3241 tcacgcacgg ccgcgttcag gtcacgctga ccgacaaccg ctacacgatg atctcggtgc
     3301 ggcggatcgg cgcccgcgac gaccgcggcg ggacaggggg ccctgccggc tctgccggcg
     3361 gggcgggggg caaagctccc cgtgggatcg agcgaagcga gatggggggt gggggccctg
     3421 ccggccctgc cggcggggcg gggggcaaag cccctcgtgg gatcgagcga cgctacgagg
     3481 tccggctcca ccacatgttc gccgacgccg acccggtgat cacccgtgcg ctggcgcact
     3541 acatcgccga caacgacgcc gatgcctcgc gcatcctggg cgacttcatc gacgccaact
     3601 ccgggcacgt ccgcggccgg tcgcggcggg cgccgtccca gctgatcctg accgccggcg
     3661 agcaccacga cctgcgcgag atcttcgacg agctcaacgc gcggtacttc gaccaccgga
     3721 tcgacgccgc gatcacctgg ggcccgcgct cgggccggcc gcggcgccgc aactcgatca
     3781 agatgggcag ctactcggtc gaggaccggc tgatccggat ccaccgctcg ctcgaccgcg
     3841 cgttcgtgcc ccggttcttc gtggcgtgga tcgtgttcca cgagatgctg caccaggtcc
     3901 acgacatccg ggtcaagaac ggccgccggg aatttcactc caaggagttc ctcgccgccg
     3961 aggccggctt cgagctctac gagcaggcga agctgtggga gcgccgccac ctcgacgcac
     4021 tgctcacgta ctgaacagat ggcgcggtcg ccggggtgac atacgccgcc gatccggtca
     4081 aggtggattt gatcgcccgc aacgtactga cgcggcgcgc gaatcagctt tctcgaggat
     4141 cggtccacat ttcacgatcg cggggtctgc cgctccctta tgctgcaaag gtggaggaac
     4201 gcatgggtga atcgatcgtc ctgggcgaac gccgccgtgc cttgcgcgtc cccgtccgcg
     4261 gattcgcggt gctctacgcc agcaccgggc cgctccacgc catgatcgag aacctgtcca
     4321 agagcggcgc gctggtcagc gtggccagcc gcctccccga ggagcagctc gacatcgaga
     4381 tccggatcgc cgagggcaac ggctgggtcg tcggccgggt cgtccgcgcc gagccccacg
     4441 cgcggcagtg gcggatcgcg gtcgagttcg gtcgggtcga cgattcgctc aaggtcgcga
     4501 tcgaggcatc gattgcctcg gcgcgcggcg cggcccgccg ccggccgatc ctcgtgatcg
     4561 acgagaacac ggatcgcaag aacagcctga tcacccggct gtccgaccgc gggatgacgc
     4621 cgctggcgcc caagaccccg ctggaggcga tcgacctcct gacccgctcg cagctccacg
     4681 tcggggtctg cctgctcgcc cccgggttcg gcgtcccgtc gcacgacctg cgcgcggtgc
     4741 tgagcgacag cttcccctgg gtctcggtcg ccgagatcac cgacgacctc gacgcgacga
     4801 cccaccacgc gatcgacacc tggaacacca cgccgatcgg tcgcatcgcc gacgcgatgg
     4861 cctgagctcg gcgctcagcg ccggccagca tacccatctt tttcgcccac ggcctcgccc
     4921 gcggtcaccg gatccgacgc gggctcagcg cccggccgct gcgaaacctt cgcagccagc
     4981 acccggccgg cgaccaggtg gtcggcaatc ccggccgcat cgtccggctg aagtccggcg
     5041 taccagaccc catccggata cacgaccgcg ttggggccgt cgaaacatgg gccgagacag
     5101 ccacaattcg tgaccagcgc ggtgaccgcg ccgcgcgcga gcagctcgcg ttgcaccgcc
     5161 gcgaccagcg cgtcaccccc ccgcggaccg caagccggct taccggatga tcggctattc
     5221 gtgcatataa acagatgtct gccggccacg cgacgaccat gttgggtaga gcacgcggtg
     5281 tcaacacctg gccggcctgg cccggccacg cggacattct ctcggtgagg ttggtcgttt
     5341 ttcgagcatt ggacacttga gcgcgtgtgg cgctcagcta acatctcgcg acctgtccac
     5401 gggggagtgg gcgcgtagcc ggatgacgcc tcaacatcac atcatcaagg agacgtcgga
     5461 gacgctgtcc cggctgctcc aggacgagtt caagcgcaac gggtacaagc gggtccacat
     5521 cgtcgagcag gccccgaagc cggacgcgat cgagggcaag ctgccggcgg tgtcggtcta
     5581 cctgtaccag atgtcgatcg accccgaggg caccgactcg acgccgcact ccgagctcgt
     5641 cgaggttccc caggaggacg gcacgatcaa ggaatacctg cgccggcgca ggctgtgggt
     5701 ccggctcgac tacctgatgt cggcgtgggc gcagaccccc gaggacgagc agctgctgct
     5761 cggcctgatc atccgcacga tcatggagaa cccggagctg ccgcgcgaga agctcaaggg
     5821 ccagtcgttc gaggacgact tcaagctcaa catcctgatg tcggcgcggc tcgacgaggg
     5881 cacgctcgca cgattctggg gaagcttgaa ccagccggtg cgtcctgcga tccaggcctg
     5941 gaccgcgatc ccgatcctcc ccgagaagct cgaggagttc aagcgcgtcc agtcgcgccg
     6001 gctcgagtac aagtcgatca ccgatccgca gatcagcgag gtggggccga gcggcaaccc
     6061 gttcctcgcc gcgcgccggc tcgacctggg aggcgggacc gggaagaagt gagcatgggt
     6121 ttcataccgc ggcgtgcatg gggcacgcgc ggtgttccga tgggcgggcg aagaccaccg
     6181 ccatcggcag cgacaaggag aacaagcgaa tggcaacgag ttatttgtcc cctggcgtct
     6241 atgtcgagga agtcgatcgc ggatccaagc cgatcgaggc cgtcggtacc aacaccgtcg
     6301 ggttcctcgg ggaatcggcc aagggcccgc tgaacgagtc ggtcctgatc accaactggt
     6361 cgcagttcgt gcggaccttc ggtgacttca aggactgcag cgagcacttc gcgcacgcgg
     6421 tgtacggctt cttcaacaac ggtggctccc ggtgcttcgt cgtcaacgtc ggcgcgcccg
     6481 agggagcgaa ggtggagacc aagccggtcg ccccggcggg tgacaagaag gaagacaagg
     6541 cgcctgccgc ggtgcacgtc ggcggcggcg gccgcgacgg gctgtacatc ggcaaggacg
     6601 gcggccccgg cgcgcgcacc ggcctcaagt gcttcgacga gatcgacgag atcgcgatcg
     6661 tctgcgcgcc cggccagacc tcgccggcgg tgcaggacgc gctgctgtcg cactgcgaga
     6721 cccgcaagga ccggttcgcg atcctcgact cgccggagac catcagcggc ggcgtcgaca
     6781 agctgcccaa gccgcgcgac tccaagtacg gcgcgtacta cttcccgtgg atccaggtct
     6841 acgacccgga caagggcaac atcttcgtcc cgccggcggg ccacatcgcc ggcgtgtact
     6901 cgcgcaccga cagcgagcgc ggcgtccaca aggcgccggc caacgagatc gtgcgcggcg
     6961 cgctcggcct caagtacaac gtgagcaagg gcgagcagga cctgctcaac cccaagggga
     7021 tcaacgcgat ccgcttcatg aacggcggca tccggatctg gggtgcccgc acgctgtcga
     7081 gcgacccgtc gtggcgctac atcaacgtgc gccggctgtt catcatggtc gagtcgagca
     7141 tcgagcgcgc cacccagtgg gtggtgttcg agcccaacga ccaccggctg tggaagcgcg
     7201 tccagcgcac gatctcgtcg ttcctgaccc tgctgtggcg caacggcgcc ctgatgggca
     7261 cttcgcccga gcaggcgttc tacgtcaagt gtgacgacga gaccaacccg cccgaggtca
     7321 tcgacgccgg ccagctggtc gtcgagatcg gcctcgcccc ggtgaagccc gcggaattcg
     7381 tcatcttccg gatcggccag atggcctccg gcggtggcgt cgaggagtag tcccaggcga
     7441 cgtcctggcg cgcctccggc cttccggggg gcgccaggcg ccgattgcgt gcacggcgca
     7501 gggaaggatc cggaggcgtg cacgtaacag gcggtgacaa gagggcgacc acaggcgatg
     7561 ccgcctgcgg cggacccgac ggctggatga aggagtcgct tcgaaaacgt caagaggatc
     7621 ttcggtgacc acaagggaaa gggacgggac acccgtggtg tcggtagcgc ggggcgttgt
     7681 ggccgccgac ggtcttcatg ccgggccggc aaaagaaccg ttcatgagtg gagagcacgc
     7741 gaatggcaga ccaaaggtca tatactggtg gtaatttccg tctcgatgtc ggcggtatcg
     7801 acgtcggtta cctgcggaag gtcagcggcg gcggcatgaa ggcggacatt gccgagcaca
     7861 agctgggccc cgacaacgtc atcaagaaga acgtcgcgaa catcgggtgg gaagaagtca
     7921 agtgtgagat gggcgtcggc atgggcaagg cggcctacga ctggatcaag tccgccttcg
     7981 ccaagcagtt cgtcacccag caggccacgc tggtcgcggg cgacttcaac cacaaggccg
     8041 tgcaggagat gcagtacatc gactgcctga tcacctcggt ggcgacgccc aagttcgacg
     8101 gctcggacaa gaacccggcg tacttcaccg tcgggttcaa gccggagcgg gtgcgccacg
     8161 tgcccggcga cggcaaggac atccgcgcca agatgggccc ggcgcagaag gcccacctgt
     8221 gctcgaactt ccggctcgag ctcggcgacc tgccgtgcaa ccgcgtcgcg tcggtcgacg
     8281 ccatggagct caagtgctcg gtggtcaagg acgacgtcgg catcacccgc gagtcgacca
     8341 tccacccggc ggcggtctcg gttcccgatc tgaaggtgac gtggtcgtac gccgacttca
     8401 ccgcggtcga gaaggaagcc cggaagtggt tcgtcgacgg cctgcacctg gaaggcaacg
     8461 agatgaccgg gcggatcgtg ctgctcgatc cgaacatgaa ggacgagatc ggcgagatca
     8521 ccctgaaggg cgtcggctgg aaggagttca agagcgacga ccgcgagggt gcctccgaga
     8581 agatcctgcg ctgcaccggc acgttctacg tcgaggagat gggcatcagc atcaagcgct
     8641 acgacgcatg atcgcgtgac agccggagca atccggtaca cggcgcgtga tacgctacgg
     8701 cctgcttggg gaaccaggca ggccgttggc tttcggcctg cgctccgagc tcagaccagg
     8761 aagagccgat ggcgttcaag actcaatatg cgtttcgctt gccgcgtggc tacgtggatg
     8821 aacagggcaa cgtccaccgc gacggcatca tgcgcctggc gaccgcgcgc gacgagatcc
     8881 tgccgctgca ggactaccgc gtgcagtcca accgcgcgta cctggtgatc gtgctgctgt
     8941 cgcgcgtgat caccaagctc ggcgaccaca cgcacgtcgc ggtcgacatg atcgagaacc
     9001 tgttctcgac cgacctcgcc tacctccagg agttctatcg caagatcaac gaggagggcg
     9061 gcgcgccgaa gcaccacatc acctgcccca agtgccagaa cgagatggac gtcgacatgg
     9121 tcaccggtgc gttgctcacc gagggaggag gtagtggcca gtctggcccg gggtgacctg
     9181 ggcggcggtc gcaacctacc cgcgcgagca gctgtaccag gagatcgcgt acatcgcgta
     9241 ccactttcac tggtccctcg acgacatcct cgacatggag cacgaggagc gccacgtctg
     9301 gctgcgcgag atcgcgcggg tgaaccgcga gatcaacgaa gcccggcgcc gctaaccccg
     9361 agcccgaagg tcccccgcca tgcgcatgcg accgtccgct gatcaacgag cacccggcat
     9421 ctaccagacc tacgacccgg tggtgatccc accgatgtcg atcgcgaaca cccggatcgc
     9481 ggggttcgtc gggatcaccc acaagggccc gatgaacgag ccgacgcggc tcggcaactg
     9541 ggacgagttc ctggagatct tcggctacag ccaggactcg tacacctcgg actcggtgta
     9601 cggcttcttc aagaacggcg gcaccgactg ctgggtcgtg cgcgtcgccc acaacccgcc
     9661 gcggggcgag atcgccggca tggagcaggc ggcgagcgcc gagcacgtcc agctcgacga
     9721 ctggaacaag ccgaccctca agatctgcgc gctcaacgag ggcagctggg gcaacgcgat
     9781 ctgggtccgc tgcgtccacg ccccggcggc gaccgcgctc ctgatcagcg acctcgacat
     9841 cggcgccggc gaggcccagg tcaacacgac ccgcggcttc gaggtcggct cgaccgtgcg
     9901 gatctacgac cgcgagaaca gcgacttcat cgtcatcacc gaggtcggcg acaagcggat
     9961 caagtggagc accgacacgc cgatcaaccg ccggcaccgc gccgccgcgc cgacccacct
    10021 cgaggtcctg accttcgaga tccacgtcgc gatgaaggac cgccgcgagg tgttcaaggg
    10081 cctgcagatg cacccgtcgt cgcgaaccta cgcgccgcgg ctggtgtccc agcgctcgcg
    10141 gctgatccgc ctggaggacc tgcgcagcaa gtcgccggtg ccgcacaacg tcccgggcgt
    10201 tctgccgatg acccggctga ccggcgggcg cgacgggctc gacgcgatca cgccggagga
    10261 cttcatcggc ctggacaacg ggcccagcga gcgcagcggc ctgctcgcgc tcgccgcgat
    10321 cgacgaggtc gccctgctgg cgtgccccga cgcgatgcgg ttctacgatc gcgagcccgg
    10381 gccggccggc gagctcaagg cccagcgggt ccaggatcag atgatctcgg tctgcgagct
    10441 ccagaaggac cgcttcgcgg tgctcgacat cccgcagagc aaggatatcg agtgggtacg
    10501 gcgctggcgc cggcgcaccg attcgtcgta ctgcgcctac tactggccgt ggttgcgcgc
    10561 ggagtcggtc gatgagaagc cgcgggtcct gccgccgtcc ggctacctgg tcggctgcta
    10621 cgcgcggcgc gacctcgatg gcgtgcatca cgcaccggcc aacctggaga tcgagggcgt
    10681 ccaggacatt gcgctccggg tgacggaaga tcacatcggc cagctcaacg ccgactcggt
    10741 caacacgttc cggattcagc gcggcgtccg gccctggggt gcacgcactg cgtcatccga
    10801 ccccgagtgg cgctacatcc cgatccgccg tcttttcatc atgttgcggc ggtcgctgga
    10861 ggccgggttt gcgtggatca cgttcgagcc caacaaccac gtcacctggg agatcgtccg
    10921 ccgccgggtg accgagttcc tggcggggtt gcatcagcag ggcatgctcg ccggagggaa
    10981 tctcgaggaa gccttcttcg taaaatgcga tgctgaaacg aatccggcag accaggtgga
    11041 taacggcatg ctgctctgtg acatcggggt cgcccccgtg caccctgccg agttcatcat
    11101 gatctctctg acccagacga tgaacacccc gacttctggc tcgtaggaga ccgaccccat
    11161 ggccacaccc acaacaaccc ggaaagatcc gctaccggtc ttctgcttcc tggtgcgctt
    11221 caccaacctg cccggcaacg tcgagggctt cttcaagagc gtcagcgggc tcaagttcga
    11281 gaccgaggtg gtcccggtga gctcgggcgg cgccaacgac acgacgttcc agctgccggg
    11341 cgcgaccaag tggggcaacc tggtgttcaa gcagggattc accaactcgt cggcgctgat
    11401 gacctggcgg cagaagtggc tccagggcca gatgacccgg accggcggca gcatcatcca
    11461 gctcgacacc tcgctcaagc ccgcggcgca gtgggacttc gtccggggct ggccgtgcaa
    11521 gtgggagatc ggcgagttcg atgcgagcaa gagcgagctc gcgatcgaga ccctggagat
    11581 cgcccacgag ggcctgacgt tctcggccgc gtgacccgcg gaccgagcac gacccagggc
    11641 actccaggac aagcggatac caagcgatag caagggacca cgatggcgaa cgaaaccgaa
    11701 ccacgcgcac tcagtgcggc gctcgcggcg ccggcgttcg tcacgcgcgg ccacggcgcc
    11761 gcgatgcacg cggctgaccg ccacatcgag cgctgggcgc ccgaggcggg tgcaggccgg
    11821 gtgcggtcga tgcgcgcgct cgggttcgtc gatcgcctcg tcgcgccgtg ggtcgagacc
    11881 gcgcagcgct cggcgtcgct gcggctgttc caccagtacg ccagcaccgg cctgggcgag
    11941 cggccgggcg gcgcggtgtc gtgggtgttc ccgcgtccgt ggtaccagga cgagctcgac
    12001 tggatggcgt ccgcacggcg cgccggtgcg caggccgggc cggcgcagac cccgagcctg
    12061 ttgaccacgc gcgggac
//
LOCUS       DASVOR010000996         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_331302,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000996 DASVOR010000000
VERSION     DASVOR010000996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331302"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="VF516_19355"
     CDS             <1..936
                     /locus_tag="VF516_19355"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8109901.1"
                     /translation="VASVFAAIVVHELGHALVARRFGIVTRDIMLYPIGGMARLERMP
                     ERPGQELLVAIVGPVINGVIALAIYLGLRLSGVGAGGDPLSIGASFAVQLMWINLSLG
                     AFNLLPAFPMDGGRILRAILAFRMDRPRATRVAARIGRGFAVVMGIAGLLWNPMLAVI
                     AVFIWLAAGQEAAMEQLKTTLRGVSVADAMVSGFETLPADAPLAAAATRLASGFQHDF
                     PVVDDGHIVGMLSRSDVRRGLAAASPDTPVAEVMHRRFPTADAGEDLDAVLGRLPPDG
                     SAVLVIDHDHPVGLLDPQHVDELLAMRQAHPRGAA"
     gene            complement(944..>1634)
                     /locus_tag="VF516_19360"
     CDS             complement(944..>1634)
                     /locus_tag="VF516_19360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109902.1"
                     /translation="VDAASFLASAWLIAGIRRPMQQAVGRRDAGSMRDALGYIRTTPV
                     VLRLVCAKGGVSSANGIVGLLPAFAASRFTGTSIATGLLFAARGLGAMIGPIAARRVI
                     GATPVRRAIVAVCGASTLTYCAVYAVFPLAHAFAAAMVLVMCAHLGGGAQWSLSTYGL
                     QRETPDHFRGRVLSLDYGLATLWIGASSIAAGLLADAWGAAPATWWLAAVGGSYGVIW
                     LGWSLAPLRTR"
BASE COUNT          213 a          617 c          598 g          206 t
ORIGIN      
        1 gtcgcgagcg tgttcgcggc gatcgtcgtc cacgagctcg gccacgcgct ggtcgcgcgc
       61 cggttcggga tcgtcacgcg cgacatcatg ctgtacccga tcggcggcat ggcgcggctg
      121 gagcggatgc ccgagcggcc gggccaggag ctgctggtcg cgatcgtcgg cccggtgatc
      181 aacggggtga tcgcgctggc gatctacctc gggctccggc tgtcgggggt gggcgccggt
      241 ggcgatccgc tgtcgatcgg cgcctcgttc gcggtgcagc tgatgtggat caacctctcg
      301 ctgggcgcgt tcaacctctt gccggcgttc ccgatggacg gcggccggat cctgcgcgcg
      361 atcctggcgt tccggatgga ccggccgcgc gcgacgcggg tcgccgcccg gatcggccgg
      421 ggcttcgcgg tcgtgatggg catcgccggc ctgctgtgga acccgatgct cgcggtgatc
      481 gcggtgttca tctggctggc ggcgggccag gaggcggcga tggagcagct caagaccacg
      541 ctgcgcggcg tgtcggtcgc cgacgccatg gtgtccggct tcgagaccct gccggccgat
      601 gcgccgctgg cggcggccgc gacccggctc gcgtcggggt tccagcacga cttcccggtg
      661 gtcgacgacg gccacatcgt cggcatgctg tcgcgcagcg acgtccggcg cggactggcg
      721 gccgcatccc cggacacccc ggtcgccgag gtcatgcacc ggcggttccc gaccgcggac
      781 gccggcgagg acctcgacgc cgtgctcggc cgcctgccac ccgacggcag cgccgtgctg
      841 gtcatcgacc acgatcaccc ggtcggcctg ctcgaccccc agcacgtcga cgagctgctc
      901 gcgatgcgcc aggcccatcc tcgcggcgcc gcgtgacccg cggtcagcgc gtgcgcagcg
      961 gggcgaggct ccagccgagc cagatcacgc cgtagctgcc gccgaccgcg gccagccacc
     1021 acgtcgccgg ggccgcgccc cacgcgtcgg cgagcaggcc ggccgcgatc gacgacgctc
     1081 cgatccacag cgtcgccagc ccgtagtcga gcgacagcac gcggccgcgg aagtgatccg
     1141 gcgtctcgcg ctgcaggcca taggtcgaca gcgaccactg cgcgccgccg ccgaggtgcg
     1201 cgcacatcac gagcaccatc gccgcggcga acgcgtgggc gagcgggaac accgcgtaga
     1261 ccgcgcagta ggtcagcgtc gacgcgccgc acaccgcgac gatcgcgcgg cgcaccggcg
     1321 tcgcgccgat cacccgccgc gccgcgatcg gaccgatcat cgcgcccagg ccgcgcgccg
     1381 caaacagaag gccggtcgcg atgctggtgc cggtgaaccg gctcgccgcg aacgccggca
     1441 gcaggccgac gatgccgttg gccgagctga ccccgccctt ggcgcacacc agccggagca
     1501 cgaccggcgt ggtgcggatg tagcccagcg cgtcgcgcat cgagccggcg tcgcgccgcc
     1561 cgaccgcctg ctgcatcggc cgccggatgc cggcgatcag ccacgccgac gccaggaacg
     1621 acgccgcatc gacc
//
LOCUS       DASVOR010000997         5265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_272745,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000997 DASVOR010000000
VERSION     DASVOR010000997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5265
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272745"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="VF516_19365"
     CDS             complement(<1..417)
                     /locus_tag="VF516_19365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109903.1"
                     /translation="MHLGPDGNPSVVYDQWYWNAVLVGDDGHTYGLETIPFQFYFAPV
                     ILDVTQIALTDAATGEYLNQLLFGGGGYTPVPDGFDIEIADSGGAFKAVGGGGSDDLT
                     FTYADGTVAQLHFEGMKNPAPAWANGIGRMIDPITGR"
     gene            882..1889
                     /locus_tag="VF516_19370"
     CDS             882..1889
                     /locus_tag="VF516_19370"
                     /inference="COORDINATES: protein motif:HMM:NF024039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8109904.1"
                     /translation="MTAAIQNVRMVMAAAAARGVPPGKLLAAIDLDPQTLIATDGRIP
                     AEAALRAWQAAGELSGDPAFGLSVVEHLASDYLGGLGFAIHASATLGGALRRIVRFFP
                     LLNQHAALELIEDGAVVRVRIAVHAEVPDQALRHPAECLLAVILRIARRTTGVNVRPA
                     AVAFRHSAPAELSVHHRVFGVVPRFDQPHHEIALDRAVLDLPHVAPDAALVSLAERYL
                     DRCAGDLPPVETFTARVRRVLLEELQLGEPTLARLAARLRMSERTVQRRLGDEGTSMQ
                     ALLDEVRCQISLRQLAESTRTIAEISYAVGFAEVRAFHRAFKRWTGSTPSAYRQSRAA
                     S"
     gene            complement(1890..2447)
                     /locus_tag="VF516_19375"
     CDS             complement(1890..2447)
                     /locus_tag="VF516_19375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109905.1"
                     /translation="MPGPDAGMTPRTCPMPMATADAGAMSAVKTQMCNYPMSAGQQHW
                     YRAFATLPGSSMSYVQIELYDKIGAFAGTTVHTGSFPVDTDPASCGVCVRGLGDKGAT
                     DAREYFATTGMVNVTAVGASGATLTVALSNIGFVELDANRHPVSGGCTATVTAGQISG
                     TIVQTGGTGGGGTGGAGMCPQTIGD"
     gene            2648..3355
                     /locus_tag="VF516_19380"
     CDS             2648..3355
                     /locus_tag="VF516_19380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109906.1"
                     /translation="MGSPRPRRDPERGNSLVLALIVMSALATLGSLTVVSMQSSIQLS
                     SNDRFQTIAMYAAESGAAVTMDFLRRNFVITSTGSTSWSNYVVPNNTPPLVNMSALIP
                     SCDALPGTPNNLFDRDQNAWFHIDLLNNRDDPSFTVGGANDTDGKVIIRSTGHGPQGA
                     LAIIEWEVQRVGFWASPNPPQPVPASPNFALPTINYNPPSFPWDGWITTRWLNPPTVT
                     DFKQVGVVLLGWRIVSL"
     gene            complement(3433..>5265)
                     /locus_tag="VF516_19385"
     CDS             complement(3433..>5265)
                     /locus_tag="VF516_19385"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8109907.1"
                     /translation="PPVDAPGVSDAALARLAEGEAIEIFDERPDKPFDRDTEVRLTGA
                     ELAARGATDLATALALLPEINVRDAGRGGYNIDIRGARKGDVTILVDGVVVSDPYYGT
                     FDVASIPITDIVQIRVATTPQSPIDGPGGPGGVIEVHTRDAIGPQLVIGRVTGSSLPI
                     VGATATARVPLARHLALRLSASGLGGVHDFDLPGDATIAERRYAATGSGRLEYRDGDL
                     RIAADGFLDDRHYLSPPSDTLLRSTILLIDRETTARASAKVDDKIGTLQIQGEASTQY
                     LERMSRFYLDPTLASAAQVENLKALRSSAMVLVTQPFLRDFRWAVSTTVDFEKAAVSD
                     IMNSTVRGTTTLVEPAADLQYERRRFRADLSAGVAVPFGVGARAWPEAKAVAKYRPTD
                     DLELTATGAYKGRVPSLRERFESAGGNPSLAPEQTAHAELRAALHLADRLHLELAPYY
                     KHASGTITTSPNPMDMGKLVNLGTVEFWGGDALARVTPIALLELGGGYAYVRARSTDP
                     SGVTHDDPLNRLPHNRWDSWIQVRPDPRYSGLARVVYFGKSIDQGLPVDGYATLQANL
                     AAQITRQYLAVLSVDDLTDVRPQTRAGYHTAGRVVSLVVQGTWE"
BASE COUNT          736 a         1889 c         1850 g          790 t
ORIGIN      
        1 gcgcccggtg atcgggtcga tcatccggcc gatcccgttg gcccacgccg gtgcgggatt
       61 cttcatcccc tcgaagtgga gctgtgccac cgttccgtcg gcgtaggtga acgtgaggtc
      121 gtcgctgcca ccgccgccga ctgccttgaa cgcgccgccg gaatcggcaa tctcgatatc
      181 gaacccgtcc ggaaccggag tgtatccgcc accgccaaac aggagctgat tcaggtactc
      241 gccggtcgcg gcgtcggtga gcgcgatctg ggtgacgtcg aggatcaccg gggcgaagta
      301 gaactggaac gggatggtct cgaggccgta ggtgtggccg tcgtcgccga cgagcacggc
      361 gttccagtac cactggtcgt acaccacgct cgggttcccg tcgggcccga gatgcacccc
      421 atcgtcctgg ggcatcgaca cggctgcgcc ggtgtgtggg ttcggcgcgg cacagcggtt
      481 gtgggcatca tcggcccaca ctcgagcggt cacacacaga acgagagcga tcacgggcga
      541 tctcttcatc ggaacttcct tgtatcgact ccgccaggcc gccgagcacg tccgggacca
      601 acgccgatcg acggcaagcg tcgagacatg ctggaatcac ccgctcgact ccctgaacgc
      661 gcagcgggtc agcgtgaacc cgtcgagatc cagcgtgcgc cggcgacgcg gctcacctgc
      721 tcgcacgtcg tcctgcaggc ggccactgga gctgtgaagg cactaccgaa tccctgggtc
      781 agccccggac atcgacctcg atccggtccg ccgtcacccg ctgtaatcgc ctgtcatggt
      841 gggcggccgc cgccggcgcg atgctgtcga taccagggat catgacggca gccattcaga
      901 acgttcgcat ggtgatggcc gcggccgcag cgcggggcgt gccgccgggc aagctgctcg
      961 ccgcgatcga cctcgatccg cagacgctga tcgcgaccga tggccggatc ccggccgagg
     1021 cggcgctgcg cgcgtggcag gcggcgggcg agctgtccgg cgaccccgcg ttcggcctgt
     1081 cggtggtcga gcacctcgcg tccgactatc tcggtggcct cggcttcgcg atccacgcca
     1141 gcgccacgct gggcggcgcg ctgcgacgga tcgtgcggtt cttcccgctc ctcaaccagc
     1201 acgcggcgct cgagctgatc gaggacggcg cggtggtccg cgtgcgcatc gcggtccacg
     1261 ccgaggtccc cgaccaggcg ctgcgccacc cggccgagtg cctgctcgcc gtgatcctgc
     1321 ggatcgcgcg gcgcaccacg ggcgtgaacg tccggccggc cgcggtcgcg ttccgccact
     1381 ccgcgcccgc cgagctgtcg gtccaccacc gggtgttcgg tgtcgtgccg cgcttcgacc
     1441 agccgcacca cgagatcgcg ctcgaccgcg cggtgctcga cctcccgcac gtcgcgcccg
     1501 acgcggcgct ggtctcgctc gccgagcgct acctcgaccg ctgcgccggc gacctgccgc
     1561 cggtcgagac cttcaccgcg cgggtccggc gggtgctgct cgaggagctc cagctcggcg
     1621 agcccacgct ggcgcggctg gccgcgcggc tgcggatgag cgagcgcacg gtgcagcgcc
     1681 ggctcggcga cgagggcacg tcgatgcagg cgctgctcga cgaggtccgc tgtcagatct
     1741 cgctgcgcca gctcgccgag tcgacacgaa cgatcgccga gatctcgtac gcggtgggct
     1801 tcgccgaggt ccgcgcgttc caccgcgcgt tcaagcgctg gaccggctcg acgcccagcg
     1861 cctaccgcca gtcccgcgcc gcgagctgat cagtcgccga tggtctgcgg gcacatcccg
     1921 gcgccgccgg tgccgccacc gccggtgccg cccgtctgca cgatggtgcc gctgatctgg
     1981 ccggcggtga cggtggcggt gcagccgccg gacaccgggt ggcggttggc gtcgagctcg
     2041 acgaagccga tgttgctcag cgccaccgtg agcgtcgcgc cgctggcgcc gaccgccgtg
     2101 acgttgacca tgccggtggt cgcgaagtac tccctggcgt cggtcgcgcc cttgtcgccg
     2161 aggccgcgca cgcacacgcc gcagctcgcc gggtcggtgt cgaccgggaa cgagccggtg
     2221 tgcacggtcg tgccggcgaa cgcgccgatc ttgtcgtaga gctcgatctg cacgtagctc
     2281 atgctcgacc ccggcagcgt ggcgaacgcc cggtaccagt gctgctgccc cgccgacatc
     2341 gggtagttgc acatctgggt cttgaccgcc gacatcgccc cggcgtccgc ggtggccatc
     2401 ggcatcgggc aggtccgcgg ggtcatgccg gcgtcggggc ccggcatgtc gtcaccgccg
     2461 ctgccgccgg gcgaagttcc agagcaaccc acggagacac agacgaggag caggacatgg
     2521 cgcatggggc acgccacgag caaccgccat gccccgcgta acccgccggg accgagttac
     2581 aggcctgtga tcgcggggat ccggttgggg ggcgcaatcc cagccagaac cccgtacact
     2641 cggctcgatg ggatcgcctc gcccacgccg cgaccccgag cgcggcaact cgctggtgct
     2701 cgcgctgatc gtgatgtcgg cgctcgcgac gctgggcagc ctcacggtgg tcagcatgca
     2761 gtcgagcatc cagctgtcgt ccaacgatcg cttccagacc atcgcgatgt acgcggccga
     2821 gagcggcgcc gcggtcacga tggacttcct gcgccggaac ttcgtgatca cctctaccgg
     2881 gtcgaccagc tggagcaact acgtggttcc caacaacacg cctccgctgg tcaacatgtc
     2941 tgcgctcatc cccagctgcg acgcgctgcc ggggaccccc aacaacctgt tcgatcgcga
     3001 ccagaacgcc tggttccaca tcgacctgct caacaaccgc gacgacccga gcttcacggt
     3061 cggcggcgcc aacgacaccg acggcaaggt catcatccgg tcgaccggcc acggcccgca
     3121 gggcgccctg gcgatcatcg aatgggaagt ccagcgcgtg ggcttctggg cgagcccaaa
     3181 cccgccgcag ccggtgcctg cctcgccgaa ctttgccctt cccaccatca actacaatcc
     3241 gccgtcattc ccctgggacg gctggatcac cacgcggtgg ctcaacccgc cgaccgtcac
     3301 cgacttcaag caggtcggcg tggtgctgct cggctggcgg atcgtgtcgc tgtagcgggc
     3361 cgctacagca ggctgaccgg gaagagcagc gctgatcgtc gtggcggccg cgcctgaacc
     3421 cggcgccacg ccctactccc atgtgccctg gacgacgagc gagaccacgc ggccggcggt
     3481 gtggtagccg gcgcgggtct gcggccggac gtcggtgagg tcgtcgacgc tcagcaccgc
     3541 gaggtactgc cgggtgatct gcgccgccag gttggcctgc agcgtggcgt agccgtcgac
     3601 cggcaggccc tgatcgatcg actttccgaa gtacacgacg cgcgcgagcc ccgagtaccg
     3661 gggatcgggg cggacctgga tccagctgtc ccagcggttg tggggcagcc ggttgagcgg
     3721 atcatcgtgg gtcacgccgc tcggatcggt cgagcgtgcg cggacgtacg cgtagccgcc
     3781 gcccagctcc agcagggcga tcggcgtcac ccgggccagc gcgtcgccgc cccagaactc
     3841 gacggtgccg aggttgacca gcttgcccat gtccatcggg ttgggcgacg tcgtgatcgt
     3901 gccgctcgcg tgcttgtagt acggcgcgag ctccaggtgc aggcggtcgg cgaggtggag
     3961 ggccgcgcgc agctcggcgt gtgcggtctg ctcgggcgcg aggctggggt tgccgcccgc
     4021 gctctcgaac cgctcgcgca ggctcggcac ccggcccttg tacgcgccgg tcgcggtgag
     4081 ctccaggtcg tcggtcgggc ggtacttcgc caccgccttg gcctcgggcc acgcccgggc
     4141 gccgaccccg aacggcaccg cgacgccggc cgacagatcg gcgcggaacc ggcggcgctc
     4201 gtactgcaga tcggccgcgg gctcgacgag cgtggtggtg ccgcgcaccg tgctgttcat
     4261 gatgtccgac accgccgcct tctcgaagtc gacggtggtc gacaccgccc accggaagtc
     4321 gcgcaggaac ggctgggtca ccagcaccat cgcgctgctg cgcagcgcct tgaggttctc
     4381 gacctgcgcg gcgctggcca gcgtcggatc gaggtagaac cgcgacattc gctccaggta
     4441 ctgggtcgag gcctcgccct ggatctgcag cgtgccgatc ttgtcgtcga ccttggccga
     4501 cgcgcgcgcg gtggtctcgc ggtcgatcag caggatcgtg ctgcgcagca gggtgtcgct
     4561 cggcggcgac aggtagtggc gatcgtcgag aaagccgtcg gctgcgatcc ggagatcgcc
     4621 gtcgcggtac tcgaggcggc ccgagccggt cgcggcgtag cggcgctcgg cgatcgtcgc
     4681 gtcgccgggc agatcgaagt cgtgcacgcc gcccaggccg gacgccgaca gccgcagcgc
     4741 gaggtgcctg gccagcggga cccgcgcggt ggcggtggcg ccgacgatcg gcagcgagct
     4801 gccggtgacc cggccgatca ccagctgcgg gccgatcgcg tcgcgggtat ggacctcgat
     4861 caccccgccc gggccacctg gcccgtcgat cggcgactgc ggcgtggtcg cgacccggat
     4921 ctgcacgatg tcggtgatcg ggatgctcgc gacgtcgaac gtgccgtagt acggatcgct
     4981 caccaccacg ccgtcgacca ggatggtgac gtcgcccttg cgcgcgccgc ggatgtcgat
     5041 gttgtagccg ccgcggccgg cgtcgcgcac gttgatctcg ggcagcagcg cgagcgcggt
     5101 cgcgaggtcg gtcgcgccgc gcgccgccag ctcggcgccg gtcagccgga cctcggtgtc
     5161 gcgatcgaac ggcttgtcgg gccgctcgtc gaagatctcg atcgcctcgc cctcggcgag
     5221 ccgggccagc gcggcgtcgc tgaccccagg cgcgtccacc ggggg
//
LOCUS       DASVOR010000998         4754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_360822,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000998 DASVOR010000000
VERSION     DASVOR010000998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360822"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(321..593)
                     /locus_tag="VF516_19390"
     CDS             complement(321..593)
                     /locus_tag="VF516_19390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109908.1"
                     /translation="MLADRGRDALEGFEPRTSRPTDPLVELDPGQPLISAVENADQGI
                     LESGTKAALRKGYAALVEQYGAKLDAFWSKQEHQDAYPECYARHDG"
     gene            complement(738..1634)
                     /locus_tag="VF516_19395"
     CDS             complement(738..1634)
                     /locus_tag="VF516_19395"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8109909.1"
                     /translation="MYREQFPGLVPGRQSSSGSLGVEQLAPGKRSLTEGLAVPRREAA
                     DPGHDTAVSEAAAAAPAPLPVTASTGPRPTLQMLFGVQRVAIAAPADDPAHVHAAAAR
                     GTATPATRLPYADQIQRVFGRHDISGIEAHVGPDAAASAHAMGARAYATGDHVVLGEG
                     ADLHTVAHEAAHVVQQRGGVHLKGGVGAVGDHYEQHADAVADRVVAGQSAEALLDEHA
                     PAPTSGAGQHPAIQRVQVTDSRDGKVIETEEVLQQEGAEGLAGLALQFYNLHNMGELK
                     RWLSSRFVGGSRMDMHRVETNA"
     gene            1797..3551
                     /locus_tag="VF516_19400"
     CDS             1797..3551
                     /locus_tag="VF516_19400"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HEX8109910.1"
                     /translation="MTSRHRPVSLGPTEVDVIHRPDGAVVLRSPHPLGPFPERITDRL
                     DHFAACDPDRLLFAQRAGGSGWRTLTYGVARDAARRIAGALLARKLSAERPVVVLSGN
                     DIEHALIELGALYAGIAYAPISPAYSLVSSDHGKLRAILALLTPGLVYASDRAAFARA
                     IAAAVPADVEVCCADDFPRLRAAPPSRAVDEAHDGVTGETIAKFLFTSGSTGDPKAVI
                     NTQRMWCSNQEMIRTVLAYFRDEPPVIVDWAPWHHTAAGNHDFGLVLYNGGTYYIDEG
                     RPVPGAIEATVRNLGEISPTWYFNVPRGFEALLPFLRADARLRATFFRRLKVLWFAGA
                     GLAQPVFDAYRELGRSTVGEEILFLTGLGSTETAPFALGRVWPTDDASNIGVPPPGVE
                     VKLVPIEGKYEARLKGPNVAPGYWRQPELTRQAFDDEGYYRLGDTFVFADGDPRRGLL
                     FRGRMSEDFKLSTGTWVHVGPLRTRLLEHLAPLVQDVVIAGEGRSELAILVFPAAGFS
                     PEAFWQRLACFESTGSSDRIVRALVMTEPPSLDAGEITDKGTINQRAVLTRRCDRVEQ
                     LYSGEPRPPAGSRARPKP"
     gene            complement(3574..4227)
                     /locus_tag="VF516_19405"
     CDS             complement(3574..4227)
                     /locus_tag="VF516_19405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109911.1"
                     /translation="MLAVAPARLATRCAWLDGPKGERQEVVMQHIVRFTRWFIMLALL
                     PLAAPGCVAEPDDADNSTATIRSELGEAGCETAIKSADGTWGIESISAGNMYDPAGCP
                     DQYLVGWGSLPAGQGAFADVFWEGTVPTLSECPNSHLSIGCYSESLGSWTITSTQVFD
                     GFWSGSSCDFLLSSGPFTCPLNGDQKNRTAAKAWVRTCSGGTCTQSKRKVGVRAWLN"
     gene            complement(4355..>4754)
                     /locus_tag="VF516_19410"
     CDS             complement(4355..>4754)
                     /locus_tag="VF516_19410"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HEX8109912.1"
                     /translation="RVCGAAHAGWRGTVAGIARNMVAAMQALGACADRIRVALGPSIG
                     PCCFEVGPEVVAAFRGALGDVPGLVVAGPHKDHIDLRVASRTILERAGVAPDHIDDRP
                     PCTRCEPERFFSYRRDGRAGGIHMSFIGLV"
BASE COUNT          651 a         1701 c         1665 g          737 t
ORIGIN      
        1 ggccgccgac ggccggcagc acacctgcgc gctcatcgcg cgggtcggcg ccgatggcgt
       61 gcgcgccgtc gccgtgcggc gcgatccgcg gctgtagcgc accagcgtcg ccgtacatcc
      121 gcggcgaacc tgaaaggcac cgacggtcgg gcgagcgtcg cacagcgcac gagcgacctg
      181 gcgggtttcc cggggccgcg cccgcagagt ggcggacgcg cgcgtagccg gtccagccgt
      241 caagtccggt ggcttccccc gggtcgcgct gtccgatccg gcggatgcgc ggcgaaccgc
      301 acgtctgcac ggcgggcgcc tcagccgtcg tgtctggcgt aacactccgg atacgcgtcc
      361 tgatgttcct gtttcgacca gaacgcatcg agcttggccc cgtattgctc gacgagcgcg
      421 gcgtagccct ttcgcagagc agctttggtt cccgactcaa ggatgccctg gtccgcgttc
      481 tcgactgccg agatgagtgg ctggcccggg tcaagctcga ccagtggatc cgttgggcgc
      541 gaggttcgcg gctcgaaccc ttcaagcgca tcgcggccac ggtccgcgag cacacggagg
      601 gcatcatcac ctacgtcacc tctggcctct cgaacggccg aaccgaggga ctcaacggca
      661 aggctcgtac gatcacgcgc cgcgcctatg gcttccacaa tgcccatgga ctcatcgctc
      721 tgctcaagct ctgttgctca ggcattcgtc tcgacccggt gcatatccat cctggatcca
      781 cccacaaacc tagaggagag ccatcttttc agctccccca tgttgtgcag gttgtagaac
      841 tggagcgcca gtccagcgag cccctccgcg ccctcctgct gcagaacttc ctcggtctcg
      901 atcaccttgc cgtcgcgaga gtccgtgacc tgtaccctct ggatcgccgg gtgctgcccg
      961 gcgccgctgg tcggcgcggg cgcgtgctcg tcgagcagcg cctcggccga ctgcccggca
     1021 accacccggt cggcgaccgc atccgcgtgc tgctcgtaat ggtcgcccac cgcgcccacg
     1081 ccccccttga ggtgtacgcc accgcgctgc tggacgacgt gggccgcttc gtgggccacg
     1141 gtgtggaggt ccgcgccctc gccgagcacg acgtgatcac ccgtcgcgta cgcccgggcg
     1201 cccatcgcgt gggcgctcgc cgccgcgtcc gggccgacgt gtgcctcgat cccggagatg
     1261 tcgtggcggc cgaacacgcg ctggatctgg tcggcgtacg gtagccgcgt tgccggtgtc
     1321 gccgtaccgc gcgcggcggc ggcgtgcacg tgcgccgggt catcggccgg cgcggcgatc
     1381 gcgacgcgct ggaccccgaa cagcatctgc agcgtcggcc gtgggccggt gctcgccgtg
     1441 acaggaagcg gtgccggcgc cgctgccgcg gcctcgctca cggcggtgtc atgaccggga
     1501 tcggctgcct cgcggcgcgg taccgcgagg ccttcggtca gcgagcgctt gccgggcgcg
     1561 agctgctcga cgccgagcga cccgctcgag ctctgccttc ccgggaccag accggggaat
     1621 tgttctcggt acatgcccat cctccacccg gcgcgccctg ggtggccagg ccggttcggt
     1681 cacgataggt agaccgtcgc tgccggtcca tgtcggccgg ttacgcggca tgacagccgt
     1741 cggcggcgct gctctcgttg ccccctggag ttccgcctgc tctagagtac cggttgatga
     1801 cctcgcgcca tcgtccggtc tcgctcggac ccaccgaggt cgacgtgatc catcggcccg
     1861 acggcgccgt cgtcctccgg tcgccccacc cgctggggcc gtttcccgag cggatcaccg
     1921 accggctgga ccacttcgcg gcctgcgacc cggatcgcct gctgttcgcc cagcgcgccg
     1981 gcggcagcgg ctggcgcacc ctcacctacg gcgtagcacg cgacgccgcc cgccgcatcg
     2041 ccggagcgct gctggcgcgc aagctgtccg ccgagcggcc ggtggtggtg ctgtccggaa
     2101 acgatatcga gcacgcgctg atcgagctgg gcgccctcta cgccggcatc gcctatgcgc
     2161 cgatctcacc cgcgtactcg ctggtgtcgt ccgatcacgg caagctgcgc gcgatcctgg
     2221 cgttgctcac gccggggctg gtctacgcct cggaccgcgc ggcgttcgcc cgggcgatcg
     2281 cggcggcggt gccggcggac gtcgaggtct gctgcgcgga cgatttcccg cgcttgcggg
     2341 cggccccgcc gtcgcgcgcg gtcgacgagg cgcacgacgg cgtcaccggc gagacgatcg
     2401 ccaagttcct gttcacgtcc ggatcgacgg gcgatccgaa ggcggtgatc aacacccagc
     2461 gcatgtggtg ctcgaaccag gagatgatcc gcaccgtgct cgcgtacttt cgcgacgagc
     2521 cgccggtgat cgtcgactgg gcgccctggc atcacacggc cgccggcaac cacgacttcg
     2581 gcctggtgct ctacaacggc ggcacgtatt acatcgacga gggccggccg gtgcccggcg
     2641 cgatcgaggc cacggtgcgc aacctcggcg agatctcgcc gacctggtac ttcaacgtgc
     2701 cgcgcggctt cgaggcgctg ctgccgtttc tgcgcgccga cgcccggctg cgcgcgacgt
     2761 tcttccggcg gctcaaggtg ctgtggttcg ccggcgccgg gctggcgcag ccggtgttcg
     2821 acgcgtaccg cgagctcggc cggagcacgg tgggcgagga gatcctgttc ctcaccggcc
     2881 tgggctcgac ggagacggcg ccgtttgcgc tcgggcgggt gtggccgacc gacgacgcca
     2941 gcaacatcgg cgtgccgccg ccgggggtcg aggtgaagct ggtgccgatc gagggcaagt
     3001 acgaggcgcg gctcaaaggc ccgaacgttg cgcccgggta ctggcggcag cccgagctga
     3061 cgcgccaggc gttcgacgac gagggctact atcgcctcgg cgatacgttc gtgttcgcgg
     3121 acggcgatcc gaggcggggc ctgttgtttc gcggccggat gtcggaggac ttcaagctgt
     3181 cgaccgggac gtgggtgcac gtcgggccgc tgcggacgcg cctgctcgag cacctcgcgc
     3241 cgctcgtgca ggacgtggtg atcgccggcg aggggcggag cgagctcgcg atcctggtgt
     3301 tcccggccgc cggattctcg cccgaggcgt tctggcagcg cctggcctgc ttcgagagca
     3361 cgggcagctc cgatcgcatc gtgcgtgcgc tggtgatgac cgagccgccg tcgctcgacg
     3421 ccggggagat caccgacaag ggcacgatca accagcgggc ggtcttgacc cggcggtgcg
     3481 atcgggtcga gcagctgtac agcggcgagc cccggccgcc cgccggatcg cgagcgcggc
     3541 cgaagccttg acgctcaccg atcggcatgg gttctagttc agccaggccc tgaccccgac
     3601 cttgcgcttg gattgcgtgc aggtgccgcc ggagcaggtg cgaacccacg ccttggccgc
     3661 ggtccggttc ttctgatcgc cgttgagggg acacgtgaac gggcccgagc tgaggagaaa
     3721 gtcgcagctg ctgcccgacc agaaaccgtc gaacacctgc gtcgaggtga tggtccacga
     3781 acccagactc tcgctgtagc agccgatcga caggtgcgag ttcggacact cgctcagggt
     3841 cggcacggtg ccttcccaga acacatcggc gaaggcgccc tgtcccgccg gcaagctgcc
     3901 ccagcccacg aggtactggt cggggcagcc tgcaggatcg tacatgttgc ccgccgagat
     3961 cgactcgatg ccccaggttc catccgcgct cttgatggcc gtctcgcagc cggcctcgcc
     4021 gagctccgag cggatcgtcg cggtggagtt gtcggcgtcg tccggttccg cgacgcaccc
     4081 cggcgcggcc agcgggagca acgcgagcat gatgaaccag cgagtgaatc tgacgatgtg
     4141 ctgcatgacg acctcctgtc tttcgccttt cggaccatcc agccacgcgc atcgcgtggc
     4201 gagtcgtgca ggcgcgactg caagcaatgc gccgcttcac tcgcgatgca ttccgccgcg
     4261 acgaccgtcc ggcgaggccg cgcgggtgcg cggatcgcgc aacccgatgg cgcagcgatc
     4321 gtgcgcgaat cgcgcaccgc cgggcgtggc gagctcagac caggccgatg aacgacatgt
     4381 ggatgccgcc ggcgcggcca tcgcggcggt agctgaagaa ccgctcgggc tcgcagcgcg
     4441 tgcacggcgg ccggtcgtcg atgtgatcgg gcgcgacgcc ggcgcgctcg aggatcgtcc
     4501 ggctcgcgac ccggagatcg atgtggtcct tgtgcggccc ggccacgacc aggccgggga
     4561 cgtcgccgag cgcgccgcgg aacgcggcga cgacctcggg gccgacctcg aagcagcacg
     4621 gcccgatcga cgggccgagc gcgacgcgga tccggtcggc gcacgcgccg agcgcctgca
     4681 tcgccgcgac catgttgcgc gcgatgcccg cgaccgtgcc gcgccagcca gcgtgggccg
     4741 caccgcacac ccgc
//
LOCUS       DASVOR010000999         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_39377,
            whole genome shotgun sequence.
ACCESSION   DASVOR010000999 DASVOR010000000
VERSION     DASVOR010000999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39377"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1706
                     /gene="uvrC"
                     /locus_tag="VF516_19415"
     CDS             <1..>1706
                     /gene="uvrC"
                     /locus_tag="VF516_19415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEX8109913.1"
                     /translation="ADVETIVVSSSKEALLLENHLIKKHQPRFNVKLRDDKQYLVLRL
                     VDPEPEGDGVPRRAVFPRVEVVRNIRDDGASYFGPYHSATSARETLRTLNRHFQLRTC
                     TDHVLETRGRPCLQYQIKRCSGPCAFAGLAGAYAEQVEDVKMFLSGKDAELLTRLRDR
                     MAARAEREDFEVAAVLRDSIAAVERTLAKQHIVQDEFVDQDVWGLAREADVAEVVVLF
                     IRGGKLVGRRAFQQKDQELPDATVIAEHLQQYYATGTFVPDEVVVGTELEDAELLGDW
                     LSSVRGKRVKIVEPRRGIRARLVELADRNAQASAASRHGKDADADALLDKVARRLALP
                     RPPRRIECFDIAHIQGSETVAAMVTFIDGVPARNLYRKFKVRTVDNNDFAAMYEVLTR
                     RFKRAGDAAGEPDDAWALPDLLVIDGGKGQLGMAVAALTDLGVALGGESGLEVIGLAK
                     ERELDAGSAPDRIYRRTVKDSIPLRQNSPELYVLARIRDEAHRFANTFHRDRRSKATL
                     RSELDDIPGIGATRRQRLLKQFGSVRAIRQASVDELARAPGMNRKAAEQIADYFARRA
                     AA"
BASE COUNT          265 a          635 c          593 g          213 t
ORIGIN      
        1 gccgacgtcg agaccatcgt ggtcagctcg tccaaggagg cgctgctcct cgagaaccac
       61 ctgatcaaga agcaccagcc gcggttcaac gtcaagctgc gcgacgacaa gcagtacctg
      121 gtgctgcggc tggtcgatcc ggagccggag ggcgacggcg tgcccaggcg cgcggtgttc
      181 ccgcgggtcg aggtcgtgcg caacatccgc gacgacggcg ccagctactt cgggccgtat
      241 cactcggcga cctcggcgcg cgagaccctg cgcacgctca accgccactt ccagctccgg
      301 acctgcaccg accacgtgct cgagacccgc ggccggccgt gcctgcagta ccagatcaag
      361 cgctgctccg gcccgtgcgc gttcgccggg ctcgccggcg cctacgccga gcaggtcgag
      421 gacgtcaaga tgttcctgtc gggcaaggac gccgagctgt tgacccggct gcgcgaccgg
      481 atggcggcgc gcgccgagcg cgaggacttc gaggtcgcgg ccgtgctgcg cgactcgatc
      541 gccgcggtcg agcgcacgct cgccaagcag cacatcgtgc aggacgagtt cgtcgaccag
      601 gacgtgtggg gcctggcgcg cgaggccgac gtcgccgagg tcgtcgtgct gttcatccgc
      661 ggcggcaagc tggtcggccg gcgcgcgttc cagcagaagg accaggagct gcccgacgcc
      721 accgtgatcg ccgagcacct ccagcagtac tacgcgaccg gcacgttcgt ccccgacgag
      781 gtcgtcgtcg ggaccgagct cgaggacgcc gagctgctcg gcgactggct gtcgtcggtg
      841 cgcggcaagc gggtcaagat cgtcgagccg cggcgcggca tccgggcccg gctggtcgag
      901 ctcgccgatc gcaacgccca ggcctcggcg gcgtcgcgcc acggcaagga cgccgacgcc
      961 gacgcgctgc tcgacaaggt cgcgcgccgg ctcgcgctgc cccgcccgcc gcgccggatc
     1021 gagtgcttcg acatcgcgca catccagggc agcgagaccg tcgccgcgat ggtcacgttc
     1081 atcgacggcg tcccggcgcg caacctgtac cgcaagttca aggtcaggac ggtcgacaac
     1141 aacgacttcg ccgcgatgta cgaggtgctg acccggcggt tcaagcgagc gggggacgcg
     1201 gccggcgagc ctgacgacgc ctgggcgctg cccgacctgc tggtgatcga cggcggcaag
     1261 ggccagctcg gcatggcggt cgccgcgctc accgacctcg gcgtcgcgct cggcggtgag
     1321 agcggcctcg aggtcatcgg cctggccaag gagcgcgagc tcgacgccgg cagcgccccc
     1381 gaccggatct accgccgcac cgtcaaggac tcgatcccgc tgcgccagaa ctcccccgag
     1441 ctctacgtgc tcgcacggat ccgcgacgag gcccaccggt tcgccaacac gttccaccgc
     1501 gatcgccgca gcaaggccac gctgcgcagc gagctcgacg acatccccgg catcggcgcg
     1561 acccgccgcc agcgcctgct caagcagttc ggcagcgtgc gcgcgatccg gcaggccagc
     1621 gtcgacgagc tcgcccgcgc gcccggcatg aaccgcaagg ccgccgagca gatcgccgac
     1681 tacttcgcgc gccgcgccgc ggccga
//
LOCUS       DASVOR010001000         2621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_272784,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001000 DASVOR010000000
VERSION     DASVOR010001000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272784"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF516_19420"
     CDS             complement(<1..405)
                     /locus_tag="VF516_19420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109914.1"
                     /translation="MAHAAGLHAALDHPDVARFLPAPDVTTLDALRARIERLAADPSP
                     APAGERSETGGGDPDGSAGGAGGRAPRGIDFLAVTHSDEAPSRRWCESFGGAGGVQLI
                     TDPDRARYAAWGVGLSDRRHFAGPESLGALRRL"
     gene            complement(456..>2621)
                     /gene="lon"
                     /locus_tag="VF516_19425"
     CDS             complement(456..>2621)
                     /gene="lon"
                     /locus_tag="VF516_19425"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX8109915.1"
                     /translation="PVKVLVEGRARARIRGFAQSEPFFVCDVEDVEETDDDAIEIQAL
                     VRSVQDVFEDYVKLNTRIPPEMLVSVRTIEEPGRLADTIVAHVALKLKDKQELLETPS
                     PARRLERLGELMQGEIEIRQVEKKLRSRVKKQMEKQQKEFYLNEQMQAIQKELGGERD
                     EFKNELNELEQKIKQKKMSKEAKDRCLKELKKLKQMSPMSAEATVVRNYLDWVIALPW
                     EDKTQDRHDINEAEAILEAEHYGLKKVKERILEYLAVQALVPRQRGPILCLVGPPGVG
                     KTSLARSIAHATDRRFVRQSLGGVRDEAEIRGHRRTYIGALPGKIIQSLKKVGSQNPV
                     FLLDEIDKMSMDFRGDPASALLEVLDPEQNNNFNDHYLDLDYDLSDVMFVTTANQQHA
                     IPLPLQDRLEIIELPGYTEWEKLAIARQYLIPKQAEANGIKDLAVNWTDDGLTAIIHR
                     YTKEAGVRNLEREIATVCRKIAKEWLASGRATTGFEVTPDKLPHWLGIEKYREVRREK
                     EDEIGLANGLAVTMFGGDLLTAEVTIVPGKGKLTLTGKLGEVMQESAQAAMSYLRSRA
                     GTFGLSNDFQNKVDIHVHFPEGAIPKDGPSAGITMATAMASALLRIPVRQTIAMTGEV
                     TLRGRVLPIGGLKEKILAAHRADVTTVLIPEDNRKDLKDVPEAVLEQLSVIGVKHMDE
                     VLRHALAHPQPDEFLREPSSVVDWRIVETMPPADPREAH"
BASE COUNT          328 a          810 c         1010 g          473 t
ORIGIN      
        1 caggcggcgc agcgcgccga gcgactcggg gccggcgaag tggcggcgat cgctgaggcc
       61 gacgccccac gcggcgtagc gcgcgcgatc gggatcggtg atcagctgga cgccgcccgc
      121 cccgccgaac gactcgcacc agcgccgcga cggcgcctcg tcgctgtggg tcacggcgag
      181 gaagtcgatc ccacgggggg ctctgccccc cgccccgccg gcggagccgt cggggtcccc
      241 accccccgtc tcgctgcgct cgccggcggg cgccggggat gggtcggcgg cgaggcgctc
      301 gatgcgcgcg cggagggcgt cgagggtggt gacgtcaggg gcggggagga accgcgcaac
      361 gtccggatga tcgagcgcgg cgtggaggcc ggctgcgtgg gccaccgaga gcggctcgat
      421 ggcgaggcgc gaggtggtga acgcgtcggc cggggtcagt gcgcctcgcg cggatcggcg
      481 gggggcatgg tctcgacgat gcgccagtcg acgacgctcg acggctcgcg caggaactcg
      541 tcgggctgcg ggtgggcgag ggcgtggcgc aggacctcgt ccatgtgctt gacgccgatc
      601 acgctgagct gctcgaggac ggcctcgggg acgtccttga ggtccttgcg gttgtcctcg
      661 gggatgagga cggtggtgac gtcggcgcgg tgggcggcga ggatcttctc cttgaggccg
      721 ccgatcggca ggacgcggcc gcgcagggtg acctcgccgg tcatggcgat ggtctggcgg
      781 acggggatgc ggagcagggc gctggccatg gcggtggcca tggtgatgcc ggcgctcggt
      841 ccgtccttgg ggatcgcgcc ctcggggaag tggacgtgga tgtcgacctt gttctggaag
      901 tcgttggaca ggccgaaggt gccggcgcgc gagcgcaggt agctcatggc ggcctgcgcg
      961 ctctcctgca tgacctcgcc gagcttgccg gtgagggtca gcttgccctt gccggggacg
     1021 atggtgacct cggcggtgag caggtcgccg ccgaacatgg tgacggcgag gccgttggcg
     1081 aggccgatct cgtcctcctt ctcgcggcgg acctcgcggt acttctcgat gccgagccag
     1141 tgggggagct tgtcgggggt gacctcgaag ccggtggtgg cgcggccgga ggcgagccac
     1201 tccttggcga tcttgcggca caccgtggcg atctcgcgct cgaggttccg cacgccggct
     1261 tccttggtgt agcggtggat gatcgcggtg aggccgtcgt cggtccagtt gaccgcgagg
     1321 tccttgatgc cgttggcctc ggcctgcttg gggatcaggt actggcgggc gatcgcgagc
     1381 ttctcccact cggtgtagcc ggggagctcg atgatctcga ggcggtcctg cagcggcagc
     1441 gggatggcgt gctgctggtt ggccgtggtg acgaacatga catcggacag gtcgtagtcg
     1501 agatcgagat agtggtcgtt gaagttgttg ttctgctcgg ggtcgaggac ctcgagcagg
     1561 gcgctggcgg ggtcgccgcg gaagtccatc gacatcttgt cgatctcgtc gagcaggaac
     1621 accgggttct gcgagccgac cttcttgagc gactggatga tcttgccggg gagcgcgccg
     1681 atgtaggtcc ggcggtggcc gcggatctcg gcctcgtcgc gcacgccgcc gagcgactgg
     1741 cggacgaacc ggcggtcggt ggcgtgggcg atcgagcggg cgagcgaggt cttgccgacg
     1801 ccgggcgggc cgaccaggca gaggatcggg ccgcgctggc gggggacgag ggcctggacg
     1861 gcgaggtact cgaggatgcg ctccttgacc ttcttgagac cgtagtgctc ggcctcgagg
     1921 atggcttccg cctcgttgat gtcgtggcgg tcctgggtct tgtcctccca cggcagggcg
     1981 atcacccagt cgaggtagtt gcgcacgacg gtggcctcgg cgctcatcgg cgacatctgc
     2041 ttgagcttct tgagctcctt gaggcagcga tccttggcct ccttgctcat cttcttctgc
     2101 ttgatcttct gctcgagctc gttgagctcg ttcttgaact cgtcgcgctc gccgccgagc
     2161 tccttctgga tcgcctgcat ctgctcgttg agatagaact ccttctgctg cttctccatc
     2221 tgcttcttga cccgggagcg cagcttcttc tcgacctggc ggatctcgat ctcgccctgc
     2281 atgagctcgc cgagccgctc gaggcggcgc gccggcgacg gggtctcgag cagctcctgc
     2341 ttgtccttga gcttgagcgc gacgtgggca acgatggtgt cggcgagccg gccgggctcc
     2401 tcgatcgtgc gcacgctgac cagcatctcg ggggggatgc gggtgttgag cttgacgtag
     2461 tcctcgaaca cgtcctggac cgagcggacc agggcctgga tctcgatcgc gtcgtcgtcg
     2521 gtctcctcga cgtcctcgac gtcgcacacg aagaacggct cgctctgggc gaacccgcgg
     2581 atccgggcgc gggcgcggcc ctcgacgagg accttgaccg g
//
LOCUS       DASVOR010001001         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_272790,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001001 DASVOR010000000
VERSION     DASVOR010001001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272790"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="VF516_19430"
     CDS             <1..677
                     /locus_tag="VF516_19430"
                     /inference="COORDINATES: protein motif:HMM:NF013265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cutinase family protein"
                     /protein_id="HEX8109916.1"
                     /translation="FGACLAGEPDPATEAADQPATTCAAVAIITARASNEAAGEGITG
                     NLVTQIINTSTQTISRAAVDYPATLNNYNSSSLQGINALKSQLTTEVPNCPNEKIVLL
                     GYSQGAHVVLDVLGGGQGGSLGTATPPISSTISSHVTAVATFGDPRHVVNQAFDLGTS
                     TRNGLFPRSSTQLQVLSGFAPKIEAFCDANDQFCDSGFSTQVHLTYLNRYQNAAASFV
                     LGKIGG"
     gene            842..1372
                     /locus_tag="VF516_19435"
     CDS             842..1372
                     /locus_tag="VF516_19435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109917.1"
                     /translation="MTKRHELRCFDYVNRPYDQVKEALSANALAIFERATRSAADRAR
                     EIGAELRVRFGAVEIAADIAIEVTEIAEARSPANQPATRLDLTWKSRRRPGLFPEMTG
                     SLWAYALSSRETQLELTGSYDPPLGVLGDAIDAIALHRLAEDSVRRFITDVASYLRAE
                     LPAEPVPGEAYAMATD"
     gene            complement(1373..>1832)
                     /locus_tag="VF516_19440"
     CDS             complement(1373..>1832)
                     /locus_tag="VF516_19440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEX8109918.1"
                     /translation="AIAERLAAIAPDAILHAGDIGDAAVIDALATTAPVLAVRGNIDT
                     RSVFPDVVTIEVIGRDLRIYLTHIAVAGPRLRGDVARAARAERAQLVVCGHSHVPFIG
                     RDRDLTVFNPGSIGPRRFHLPIVFGTIAITPQAVRLAHIDCETGQPWLPP"
BASE COUNT          293 a          695 c          606 g          238 t
ORIGIN      
        1 tgttcggcgc ctgcctcgcc ggcgagcccg atcccgcgac cgaagccgcc gatcagccgg
       61 cgacgacctg cgccgccgtc gcgatcatca cggcgcgcgc gtcgaacgag gcggccggcg
      121 agggcatcac cggcaacctg gtcacccaga tcatcaacac cagcacgcag accatctcgc
      181 gcgcggcggt cgactacccc gcgacgctga acaactacaa cagcagctcg ctgcagggca
      241 tcaacgcgct gaagagccag ctgaccaccg aggtccccaa ctgccccaac gagaagatcg
      301 tgctgctcgg ctactcgcag ggcgcccacg tcgtgctcga cgtgctcggc ggcggccagg
      361 gcggctcgct cggcaccgcg accccgccga tctcgtcgac gatctccagc cacgtcaccg
      421 cggtcgcgac gttcggcgac ccgcgccacg tcgtcaacca ggcgttcgac ctcggcacct
      481 cgacccgcaa cggcctgttc ccgcgcagct cgacccagct ccaggtgctg agcggcttcg
      541 cgccgaagat cgaggcgttc tgcgacgcca acgaccagtt ctgcgacagc ggcttcagca
      601 cgcaggtcca cctgacctac ctcaaccggt accagaacgc ggccgcgagc ttcgtgctcg
      661 gcaagatcgg cggctgatcc gccggcgtcg ccgcacagca tctcgcgcgg ttagccgccg
      721 cgccggagcc ttgcgcttgg tgcaaccgtt gcagcatcgc gattgcgatg cggaccgcga
      781 gcccacaaca cgcaaacact gcgtctcacc gggctacacg cgcaatcgcc gcggaggcaa
      841 catgaccaag cgacacgagc tccggtgctt cgactatgtg aaccgtccct atgaccaggt
      901 gaaggaggcg ctgtcggcca acgcgctcgc gatcttcgag cgcgcgacgc gctcggccgc
      961 ggatcgcgcc cgcgagatcg gcgccgagct gcgcgtccgg ttcggcgcgg tcgagatcgc
     1021 cgccgatatc gcgatcgagg tcaccgagat cgccgaggcg cgctcgcccg ccaaccagcc
     1081 ggcgacccgg ctcgacctga cctggaagtc gaggcgccgc ccgggcctgt tccccgagat
     1141 gaccggatcg ctgtgggcct acgcgctgtc gtcgcgcgag acccagctcg agctgaccgg
     1201 cagctacgac ccgccgctcg gcgtgctcgg cgacgcgatc gacgcgatcg cgctgcaccg
     1261 cctcgccgag gactcggtgc gccggttcat caccgacgtc gcgtcgtacc tgcgcgccga
     1321 gctgcccgcc gagcccgtcc ccggcgaggc ctacgcgatg gcgaccgact gatcagggcg
     1381 gcagccacgg ctggccggtc tcgcagtcga tgtgcgcgag ccggaccgcc tgcggcgtga
     1441 tcgcgatcgt gccgaacacg atcggcaggt gaaaccgccg cggtccgatc gatcccgggt
     1501 tgaacacggt cagatcgcga tcgcggccga tgaacggcac gtgcgagtgg ccgcacacca
     1561 ccagctgcgc ccgctcggcg cgcgccgccc gcgcgacatc gccgcgcagc cgcggcccgg
     1621 cgaccgcgat gtgggtcagg tagatccgga ggtcgcggcc gatcacctcg atcgtgacca
     1681 cgtcggggaa caccgaccgc gtgtcgatgt tgccgcgcac cgcgagcacc ggcgcggtgg
     1741 tcgcgagcgc gtcgatcacc gcggcgtcgc caatatcgcc ggcgtggagg atcgcgtcgg
     1801 gcgcgatcgc ggcgagccgc tcggcgatcg cc
//
LOCUS       DASVOR010001002         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_389704,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001002 DASVOR010000000
VERSION     DASVOR010001002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_389704"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="VF516_19445"
     CDS             complement(<1..1433)
                     /locus_tag="VF516_19445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109919.1"
                     /translation="MRLRVDRRSPGIRFESRPPALPDALPRMDVAVFVGFAASGPLHT
                     PVAIDDPRGFANVFGDDVALGWDGEGGGVVRGNLGPAVRSFFRNGGRRCWIVRVAGAA
                     RANRFAIPGLAAWSGGTLRPACAVARSEGSWSDDLAVSASLAAQPIDLLALELDDHGR
                     PLSAQLAVPDAVAAGDQLRITLPDSGPVLVAAIRDIDGDQISFVDDATLWFAAKLPAP
                     PDPAALAIAWFPDDDPASEPVAQPLTPAGAPRWDADTNFAPIAVPIFAPPAGAPDAPA
                     LEPGRLVRLRWGAEELWLGVDDLVATEAGSPVQPVVELRGAWSRRLTAPPGPGTITAR
                     LSAERVRITLWVQRGTELPVRLDDLGLSPGHPSYLGALPTDRMLFSTEPPTLSDRDRD
                     LRELTRQLQGVAGEAVDPRESYAALWRAVTVPRFSLAVPAVAIDAFVPIGLPLVPAAY
                     LGAHGGGQTALARDGLTEFSARLFLDP"
     gene            complement(1435..1884)
                     /locus_tag="VF516_19450"
     CDS             complement(1435..1884)
                     /locus_tag="VF516_19450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8109920.1"
                     /translation="MTPRDRPYGQFNFWVTLGQGGDPKAPDGGFQEISALGMEVGVAE
                     YRYGNHPLNNTLKVTGLGKSADVTLKRGVMGSDTVYKWFNAIRNGQNEMRTVRIELRN
                     EENSDTVQTWVLNNARVIKFTAGPLNAKSNEIAMEELTLSFERMELQ"
     gene            complement(1911..>3360)
                     /locus_tag="VF516_19455"
     CDS             complement(1911..>3360)
                     /locus_tag="VF516_19455"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HEX8109921.1"
                     /translation="VVSSLADRDIVLISRASPQSTDFYLATWVESEQTFHFTSAASGN
                     LVLTTDFVRGDQIQVITAAVAVTPSDGLGLAEVWEGLALDPAHTQNGANDSLTYMFGS
                     YVESPDQFERAHGRDLPIVVTPETAIHSGLDVLNALFGAAPGRHGSPPGAGDVLSLRE
                     LVLANVSDAARFVEIPLTGGNDGLRPTAGDYEGQADTASSVKTGLKQFEDIDQISIIA
                     APGSTFGYEAGRHDDANTIISLLIQHCEYMRYRIAVIDSGNGQTLNQVQDMRARFDSS
                     WAAMYYPWVRILDPVTRVPIFVPPSGFVSGIYARNDITRAVYKAPANEVVNLSIGFET
                     LLNKAQQDVLNPLGINCFRFFEGRGYRLWGGRTMSSDPEWKYVSLRRYFAYLEHSIDR
                     GTQWAVFEPNGPLLWNNVRQTIEDFLYNEFTSGALLGDKPDKAYFVRCDRTTMTQNDL
                     DNGRLVCLIGLCPVKPAEFVIFRIGQWTADRK"
BASE COUNT          504 a         1175 c         1186 g          495 t
ORIGIN      
        1 tcgggatcga ggaacagcct cgccgaaaac tcggtgaggc cgtcgcgcgc cagcgcggtc
       61 tgcccgccgc cgtgggcgcc gaggtaggcc gccggcacca gcggcaggcc gatcggcacg
      121 aacgcgtcga tcgccaccgc cgggaccgcg agcgagaacc gcggcacggt gaccgcgcgc
      181 cacagcgcgg cgtaagactc gcgcggatcg acggcctcgc cggcgacacc ctggagctgg
      241 cgcgtcagct cgcgcaggtc gcgatcgcga tcggacagcg tcggcggttc ggtcgagaac
      301 agcatgcgat cggtcggcag cgccccgaga tagctcgggt ggccgggcga cagcccgagg
      361 tcgtcgagcc gcaccggcag ctcggtgccc cgctgcaccc agagcgtgat ccgcacgcgc
      421 tcggccgaca ggcgggccgt gatggtcccc ggtcccggcg gcgccgtcag ccgccggctc
      481 catgcgccgc gcagctcgac gaccggctgc acgggcgacc ccgcctcggt cgcgaccagg
      541 tcgtcgacgc cgagccacag ctcctcggcg ccccagcgca gccgcaccag gcgccccggc
      601 tccagcgccg gtgcgtcggg tgcaccggcc ggcggcgcga agatcgggac ggcgatcggg
      661 gcaaagttcg tgtccgcgtc ccagcgcggc gcgccggcag gggtcagcgg ctgcgccacc
      721 ggctcgctgg ccgggtcgtc gtcggggaac catgcgatcg cgagcgccgc ggggtcgggc
      781 ggcgctggta gcttcgctgc gaaccacagg gtggcgtcgt cgacgaagct gatctggtcc
      841 ccatcgatgt cgcggatcgc ggcgaccagg accggcccgg agtccgggag cgtgatccgc
      901 agctgatcgc cggccgcgac cgcatcgggc accgccagct gggcagacag cgggcggccg
      961 tggtcgtcga gctccagcgc gagcagatcg atcggctgcg cggccagcga cgcgctcacc
     1021 gccaggtcgt ccgaccagct gccctcggat cgcgcgaccg cacaggcggg ccggagggtg
     1081 ccgccgctcc acgccgccag ccccgggatc gcgaaccggt tggctcgcgc cgcgccggcg
     1141 acccgcacga tccagcaccg gcggccgccg ttgcggaaga acgaccgcac cgcgggtccc
     1201 aggttgccgc ggaccacgcc gccgccctcg ccgtcccagc cgagcgcgac atcgtcgccg
     1261 aacacgttgg cgaacccccg cggatcgtcg atcgcgaccg gcgtgtggag cgggcccgac
     1321 gcggcgaagc cgacgaacac ggccacgtcc atgcgcggca gcgcgtccgg cagcgccggc
     1381 ggcctgctct cgaaccggat acccggcgat ctgcgatcga cgcggagccg caagttactg
     1441 gagctccatc cgctcgaagc tcaaggtcag ctcctccatc gcgatctcgt tgctcttggc
     1501 attcaacgga ccggcggtga acttgatgac gcgagcgttg ttgaggaccc aggtctgcac
     1561 ggtgtccgag ttctcctcgt tgcgcagctc gatccgcacg gtccgcatct cgttctgccc
     1621 gttgcggatc gcgttgaacc acttgtagac ggtgtccgag cccatcacgc cgcgcttgag
     1681 cgtgacgtcg gcggacttgc ccagcccggt caccttcagc gtgttgttga gcgggtggtt
     1741 gccgtagcgg tactcggcga cgccgacctc catgccgagc gcgctgatct cctggaagcc
     1801 gccgtcggga gccttgggat cgccaccctg ccccagggtc acccagaagt tgaactgtcc
     1861 atacggtcga tcgcgaggtg tcatcgatat ctcctagaag ctgagagccg ctacttgcga
     1921 tccgccgtcc actgaccgat gcggaagatc acgaattcag cgggcttgac cggacacagc
     1981 ccgatcaggc agaccagccg gccgttgtcg agatcgttct gcgtcatggt cgtgcggtcg
     2041 cagcgcacga agtacgcctt gtccggcttg tcaccgagca gcgcgcccga ggtgaactcg
     2101 ttgtagagga agtcctcgat ggtctggcgg acgttgttcc acagcagcgg gccgttgggc
     2161 tcgaacacgg cccactgcgt gccgcgatcg atcgagtgct cgagataggc gaagtagcgg
     2221 cgcaggctga cgtacttcca ctcgggatcc gagctcatcg tccggccgcc ccacagccgg
     2281 tagccgcggc cctcgaagaa ccggaagcag ttgatcccga gcgggttgag cacgtcctgc
     2341 tgcgccttgt tgagcagggt ctcgaacccg atcgacaggt tcaccacctc gttcgccggc
     2401 gccttgtaga ccgcgcgggt gatgtcgttg cgggcgtaga tcccgctgac gaagccgctg
     2461 ggcggcacga agatcggcac ccgggtgacg gggtcgagga tgcggaccca cgggtagtac
     2521 atcgcggccc agctcgaatc gaaccgggcg cgcatgtcct ggacctggtt caacgtctgc
     2581 ccgttgccgc tgtcgatcac cgcgatccgg taccgcatgt actcgcagtg ctggatcagc
     2641 aggctgatga tggtgttcgc gtcgtcgtgg cggcccgcct cgtagccgaa cgtcgatccc
     2701 ggcgccgcga tgatcgagat ctgatcgatg tcctcgaact gcttgagtcc ggtcttgacc
     2761 gacgaggcgg tgtcggcctg gccctcgtag tcgccggcgg tcgggcgcag cccgtcgttg
     2821 ccgccggtca gcgggatctc gacgaaccgc gccgcgtcgc tgacgttggc cagcacgagc
     2881 tcgcgcaggc tcaggacgtc gccggcgccc ggcggcgagc cgtgccttcc gggggccgcg
     2941 ccgaacagcg cattcagcac gtcgaggccg gagtggatcg cggtctcggg cgtcacgacg
     3001 atcggcagat cgcggccgtg cgcgcgctcg aactggtcgg gcgactcgac gtacgatccg
     3061 aacatgtagg tcagcgagtc gttcgccccg ttctgggtat gcgcggggtc gagcgcgagg
     3121 ccctcccaga cctcggccag cccgagcccg tcgctcggcg tcaccgcgac cgccgcggtg
     3181 atcacctgga tctggtcccc gcgcacgaag tcggtggtga gcacgaggtt gcccgacgcg
     3241 gcggacgtga agtggaaggt ctgctcgctc tcgacccagg tcgccaggta gaagtcggtg
     3301 gactgcgggc tggcgcgcga gatcagcacg atgtcgcggt cggcgagcga cgacaccacc
//
LOCUS       DASVOR010001003         1960 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156269,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001003 DASVOR010000000
VERSION     DASVOR010001003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156269"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VF516_19460"
     CDS             <1..302
                     /locus_tag="VF516_19460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109922.1"
                     /translation="ALAAGDADARLADQRRAAARMVEANIDQLDPRPIRDHRLQTYAR
                     PSHMAKKRQSTRPGAVIRQSGDVEQGRRICLRRTARWTSTWALTWTAIVELDVAV"
     gene            complement(683..1798)
                     /locus_tag="VF516_19465"
     CDS             complement(683..1798)
                     /locus_tag="VF516_19465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109923.1"
                     /translation="MLVSAAALAGCGDNITRAAPDAGPPPAPVVRAVVVAGDVNPAHP
                     GVLSIYDPATGVVRKNAAPAMAVGDDPVLRHFGHELFIVNRADGNNVTILDDRTLAFE
                     EQLGTGASSNPQDVAVVGAKLYVPTYGSAGVTALTRGSTATSVIDLSADDPDGKPNCE
                     SIYRVGQDLYVVCQLLDDTQQFLPPRGPGKVYVIDTATDRVRASLTLGHDNPFSLLEQ
                     VPAGAPHGGELVVGTVKFDDGSGAGCVERIVPGATPQAAGCLVDNADLGGYASRIAFQ
                     SDAGGALGLFAVPTTFPKADLRALDMPTGMLRSGALNASSEIAADVVVCPGDQIVIAD
                     PTMDANGLRVYQGSMEKTTAAQPIGLPPASTHGLVCY"
BASE COUNT          291 a          671 c          693 g          305 t
ORIGIN      
        1 acgcgctcgc cgccggtgac gcggacgctc gcctcgcgga ccagcggcgc gccgccgcgc
       61 ggatggtcga ggcgaacatc gaccagctcg atcccaggcc gattcgtgat catcgcctcc
      121 agacctacgc ccgcccatcc cacatggcaa aaaaacggca gtcaacccgg ccgggggccg
      181 tgatccgtca atcaggagat gtcgaacaag ggcggcggat ctgcctcaga cgcaccgccc
      241 ggtggacctc gacctgggcc ttgacctgga cggccatcgt tgagcttgac gtggccgtct
      301 gatgattcat cacgagttgc gcgttcgggg tggttcccgt ggcgctcgac gtcgacatcg
      361 acgatgacgt cgaccttgac tccagcgttg agcttgacgg cagcgaagcg atacgtggac
      421 gtctgacccc accgtcgccc tcgaccccgt tgtcgcgctc cttcgaggct cgcagcacag
      481 gatcggtcga cctcctcttt gaccactggt cgcgattcct cgacgaagat tcgggtgcca
      541 ccagaaggcc tacgtacaag gtcgagggtg gagtcgacgt cgtacgtcgc cgttcaggtc
      601 aacgccttgc gacaacgtcc aggtcgacct caacgtcttc gcaagcacgt cctggccttt
      661 cgatcggccc ctcttcgttc attcagtagc agaccagccc gtgcgtcgac gccggcggca
      721 gcccgatcgg ctgcgccgcg gtggtcttct ccatgctgcc ctggtagacc cggaggccgt
      781 tggcgtccat cgtggggtcg gcgatgacga tctggtcgcc ggggcacacc accacgtcgg
      841 ccgcgatctc gctgctcgcg ttgagcgcgc cggaccgcag catgccggtc ggcatgtcga
      901 gcgcgcgcag gtcggccttg gggaacgtcg tcggcaccgc gaacaagccg agcgcgccgc
      961 cggcgtcgga ctggaacgcg atccggctcg cgtagccgcc gaggtcggcg ttgtcgacca
     1021 ggcagcccgc cgcctggggc gtcgcgcccg gcacgatgcg ctcgacacag cccgcgccgc
     1081 tgccgtcgtc gaacttcacc gtgccgacca ccagctcgcc gccgtgcggc gctccggccg
     1141 gcacctgctc gagcaggctg aacgggttgt cgtggcccag ggtcaggctc gcccgcacgc
     1201 ggtcggtcgc ggtgtcgatg acgtagacct tgcccgggcc gcgcggcggc aggaactgct
     1261 gcgtgtcgtc gagcagctgg cacaccacgt agaggtcctg accgacccgg tagatcgact
     1321 cgcagttcgg cttgccgtcg gggtcgtcgg ccgacaggtc gatcaccgac gtggcggtcg
     1381 agccgcgggt cagcgcggtc acgccggcgc tgccgtaggt cgggacgtag agcttggcgc
     1441 cgaccaccgc gacgtcctgc gggttggagc tcgcgccggt gccgagctgc tcctcgaacg
     1501 ccagggtccg gtcatcgagg atcgtgacgt tgttgccgtc cgcgcggttg acgatgaaca
     1561 gctcgtggcc gaagtgacgc agcaccggat cgtcgccgac cgccatggcc ggcgccgcgt
     1621 tcttgcggac cacgccggtc gccgggtcgt agatcgacag cacgccgggg tgtgcggggt
     1681 tgacgtcgcc ggcgaccacg accgcacgca ccacgggggc cgggggcggg ccggcatcgg
     1741 gcgcggcgcg ggtgatgttg tcgccgcacc cggcgagcgc ggcggccgag acgagcaccg
     1801 aagcgagcgt ggtcgcaatc gcaatcctgg tcatgaggtc ctcgcgagct agtaggtcca
     1861 gtcgagggtg agatcgaagc tgcgcccggg cagcgggaac cgcgcgacgt cggtcagcgc
     1921 ggtgggagac tcggtgagcg acggcgacgg cggcggatcg
//
LOCUS       DASVOR010001004         2412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_506696,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001004 DASVOR010000000
VERSION     DASVOR010001004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506696"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VF516_19470"
     CDS             <1..451
                     /locus_tag="VF516_19470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109924.1"
                     /translation="AFLDVLEAPTSGALRAGLNAQFPFLAYPGGGVVKRNNQNFAVNF
                     TQPQRELIRLVNRWRNNGRLSSDNVWYDDNMRLDSNRTQIDHIIPIGFAPASSSNFYW
                     NAQVTSDQYNRSKGNQSEAGFRAAYQAAPRVLRHRCARTQLYTPYRR"
     gene            complement(681..1841)
                     /locus_tag="VF516_19475"
     CDS             complement(681..1841)
                     /locus_tag="VF516_19475"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX8109925.1"
                     /translation="MGGEEIAAFIRLALPPADPPIGVDAGLAERLRTAEQALVRLEVA
                     GEMVPSLDWFIYAFVRKEAVLSSQIEGTQATLVDLLTFEAEASAEQSARSNAEVEEVC
                     NYLDALTYSRAQLADPKGLPLSTRLLNGAHQRLMRGVRGADKLPGEVRRSQNWIGGSR
                     PGNAAYVPPPPHALGEVLGAFEKYIHADDTLPPLVRAGLLHVQFETIHPYLDGNGRIG
                     RLLVTLLLEHWKLLTKPLLYLSLFFKRHKGDYYRRLDAVRIDGDWEGWLDFFLDGIAT
                     IADEAVASARELFALVAADRARVLAHEGTSVMALRLFELLPRHPVVNVASVMKLIETT
                     KPTAGRAIDLLVAAGVIVEITGKKRDRSFVYRAYLDRLKVGTELEVAPRSMS"
BASE COUNT          406 a          815 c          820 g          371 t
ORIGIN      
        1 cgcgttcctc gacgtgctcg aggcgccgac ctccggcgcg ctgcgcgccg gcctcaacgc
       61 ccagttcccc ttccttgcct atccgggggg cggcgtggtc aagcggaaca accagaactt
      121 cgcggtcaac ttcacccagc cacagcgcga gctgatccgc ctggtcaatc gctggcgaaa
      181 caacggccgg ctgagctccg acaacgtctg gtacgacgac aacatgcgac tggactccaa
      241 tcgcacccag atcgaccaca tcatcccgat cggcttcgcc cccgcgagct cgagcaactt
      301 ctactggaac gcccaggtga cgagcgacca gtacaaccgc tccaagggca accagagcga
      361 ggccgggttc cgcgccgcct accaggcggc cccgcgcgtg ctgcggcacc ggtgcgcccg
      421 cacgcagctc tacacgccgt accggcggtg agccggcgac ccggaaggcg cacctcaacc
      481 gtcgagccgc cctacctccg gccctggccc ctggcggatc cgcgcgaccg tctcgccatc
      541 gtccggcgcg acgccgcatg gccgctgtac ggcaaccgac gagcgcagtc acaccagcga
      601 gctgcaactc gcgctcggcg acttgctcgc tggcgaggcg gagtagccga tcagcgagcg
      661 tgtcgtgggg aagtcattca ctagctcatg gacctcggtg cgacttcgag ctcggtgccg
      721 accttgagac ggtcgaggta ggcccggtac acgaaggagc gatcgcgctt cttgccggtg
      781 atttccacga tcacccccgc ggcgacgagc aggtcgatcg cgcgccctgc ggtcggtttc
      841 gtcgtctcga tgagcttcat caccgacgcg acgttcacga ccgggtggcg cggcaggagc
      901 tcgaagagcc gcagcgccat cacggacgtg ccctcgtgcg caagcacgcg cgcgcgatca
      961 gcggcgacga gcgcgaacag ctcgcgcgcc gaggcgactg cctcgtccgc gatcgtcgcg
     1021 atgccatcga ggaagaagtc gagccagccc tcccagtcgc cgtcgatgcg tactgcatcg
     1081 agccgccggt agtagtcgcc cttgtgccgc ttgaagaaca ggctcaggta caggagcggc
     1141 ttggtgagca gcttccagtg ctcgagcagc agcgtgacga gaagacgccc gatgcggccg
     1201 ttgccgtcga ggtacggatg gatggtctcg aactgtacgt ggagcagccc ggcacgcacg
     1261 agcggcggca gcgtgtcatc ggcgtggatg tacttctcga acgcaccgag cacctcgccg
     1321 agcgcgtggg ggggcggtgg tacgtaggcg gcattgcccg gccggctgcc gccgatccag
     1381 ttctggctgc gccttacctc gcccggcagc ttgtcggctc cacgcacacc gcgcatgagg
     1441 cgctgatgcg caccgttcaa cagccgcgtc gaaagcggca aacccttcgg gtccgcgagc
     1501 tgagcgcgcg agtacgtgag cgcgtcgagg tagttgcaga cctcctcgac ctcggcgttc
     1561 gaccgggcgc tctgctccgc gctggcctcg gcctcgaacg tgagcaggtc gacgagcgtc
     1621 gcctgcgtgc cctcgatctg cgacgagagg acggcttcct tgcgaacgaa ggcgtagatg
     1681 aaccagtcga gcgagggcac catctcgccc gcgacctcga ggcggacgag cgcctgctcg
     1741 gcggtgcgaa ggcgttcggc gaggccggcg tcgacgccga tcggcggatc tgccggcgga
     1801 agcgcgagcc ggatgaacgc ggcgatctct tcgccgccca ccgtggttcg ttcgtatcgt
     1861 ccggtgactc gcttcggcat cgttgggccc gtcagtaacg tgcgcttgac tagctgcgcc
     1921 ggctggtaaa gcggcggtga ccaagagaag cgctaggaac gtagccttta ctagcgaccg
     1981 acgttagtca aggagacgtt accagcgagg catggaacca gggctttccc caggagcatt
     2041 cgcaggagcg ccgcccaagg cgctctgtaa gccgttggat caacactaga atcgtccagg
     2101 acggcgactt gaagtcgtct ggtcgttctc cggcgaggaa ctccagtcgc ttgcggcgga
     2161 ggatatcgag ctcggcgagc gtcagcgcga tgcgctcgtg ccgacaggag gtccgtgtgc
     2221 tggtgacgga gggcggggtc gacgaagctg ccaggctgga gcgtgaagcg tcgaccggtc
     2281 cagcgcctcg gccaccgccg ccggcacgat ggcgcagcgc gccgcgggcg tgctcgggct
     2341 ggcggccgcg cgatgcggca cagtgtcagc gatggcgctc cgactcggcg gtctggcaga
     2401 tatcgatccg gc
//
LOCUS       DASVOR010001005         8879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_214770,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001005 DASVOR010000000
VERSION     DASVOR010001005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214770"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..945)
                     /locus_tag="VF516_19480"
     CDS             complement(<1..945)
                     /locus_tag="VF516_19480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109926.1"
                     /translation="MSTSNDARRTSPYEFSDATRHPVPARVGRYVICEMSGAGAMGVV
                     YRAYDPKLDRAVAIKLVRAGASDSDQARLSREAQTMAQLRHPNVVPIFDVGLAPGGVF
                     LAMALLDGGTLRQWFNRERHSLDTILDKFLAAGRGLAAAHSMGIVHRDFKPDNVLLDV
                     GGEVFVADFGLAQLTVRSGAVNANISFAHADTLTQSGQIMGTPAYMSPEQLRGQHVDA
                     RADQFSFCVALWEAVYGQRPFAEPADRSADPVAALLAAIAAGPVPPACGDRPRWLASL
                     LTRGLAADPDGRWPTLHALLDEIAAQRAAAARPSRRFAA"
     gene            1252..2745
                     /locus_tag="VF516_19485"
     CDS             1252..2745
                     /locus_tag="VF516_19485"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8109927.1"
                     /translation="MEGVAAGRHVLEIVGVLASETDVEAAARILLERLLEVTGAQRGF
                     IVVREADSYVDVFNVAFDRSAVSTEERRFSRRLVRMAAASREPLYSASAATDPRFASV
                     DSVASLGPRAVLVVPLCEREVVHGIVYFDSPVPIDEPARGWVIELARLTAPLLRRAVA
                     EEALRRRARALESDLLAQFDFEGIVTRDPQVLALLRIAAQVADATATVLIRGETGTGK
                     ELLARALHVNSSRRERPFVALHCAALPDTVLEAELFGHVRSAFTGADRDRAGRIASAR
                     GGTLFIDEVGEIPLGVQVKLLRFLQSGEIQRLGSDRPEQVEVRVVCATNRDLAALVKE
                     GRFREDLYYRIQVIELVLPPLRERSGDRELLVEHFLARFARQKGMALRLTAEARAALV
                     TYHYPGNARELEHAIERMALLATTPELGVELLPPAIRPGGPASTSFRDYTSDELRAAR
                     AAAVAEVEARFVEGLLQKSDGNVSAAARLAGMPRGYLQKLIARRRGR"
     gene            3368..4036
                     /locus_tag="VF516_19490"
     CDS             3368..4036
                     /locus_tag="VF516_19490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109928.1"
                     /translation="MEMRVLLSMLMICCVGCLPDPGDEDLGDAEEGGTSTVDQASVSE
                     GFVAGAGVVTDDFGDEGPVDMSSHPDSLATGMWQAILWADGAIEQNDTPFDTSDIDCQ
                     FGLNTAAATRNWQSAHGASVDGSAGPMTLGHADDRLSICINGSTSTQSVVRYSGSVHS
                     LFFIRKEGSGSVNHAYQAIGTDGKLRVISYGFISSCSSSFFLGLPSSGSLCCDGTCKP
                     GCPC"
     gene            4481..5542
                     /locus_tag="VF516_19495"
     CDS             4481..5542
                     /locus_tag="VF516_19495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109929.1"
                     /translation="MARRTLARMSMVALIVIGFTTLTTPAIASVTLPGGKARFVVAVG
                     TIPTVDGSGSGYSVRLADYTFTAGSTGTVSESFWYWSQGTMSGRSASGVTNSGCESRV
                     CDIGVPTGFEDHTRTDTAPYPKSFTGAYAYDPSTSSVVITWTDNSIERWTVDYGNDGF
                     QPIARMHIRSTNYGVLEGWAYGSNAAWTTSVDMTSIVAMHPSLHGDQYQNDYGATAIF
                     SLASLAIPATAELCNTNCFSFESQAGSGSSCVYTGADGKQHPGYYRWRFAHTSDFPPR
                     KNAYNIYCEQLNIPQSQQAITQHDDGQTCYNGNYHIRPMLQIIDDDGLFHGWVGVEAS
                     LYQAQQGSAYVQVFHFDDL"
     gene            complement(5819..6211)
                     /locus_tag="VF516_19500"
     CDS             complement(5819..6211)
                     /locus_tag="VF516_19500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109930.1"
                     /translation="MIPVAEVKERHQLTNANGRVMRDADGEPVRVNTLPGIHVSRRTF
                     NSVAAEIGIDLETREALMNHSKRGVNVKHYVQTERLDHLQASAERIERALWERIKGTR
                     PPQGRGRRDGASLRRRRQAASGQPTLLI"
     gene            complement(6217..6606)
                     /locus_tag="VF516_19505"
     CDS             complement(6217..6606)
                     /locus_tag="VF516_19505"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8109931.1"
                     /translation="MIDRALNPATRLQQAALKPRETRIPDTEFADGYAKVLKLENPVR
                     RDLHLVALFTGIRSDGVRNLRWEDVDFDAELIQVARTKGDRPYSLPMVKTVRKILQRR
                     KADNATSPLLARFGGDHASSSLPCRAT"
     gene            6819..8048
                     /locus_tag="VF516_19510"
     CDS             6819..8048
                     /locus_tag="VF516_19510"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX8109932.1"
                     /translation="MDGPAVSLKFHLAQSDAVAWLRTLPSESVDLVVTDPPYESLEKH
                     RAIGTTTRLKHSKASSNDWFAIFPNERFPELFAEVYRVLRRNTHFYLFCDPETMFVAK
                     PAGEQAGFKFWKPLIWDKCLGPDTRLWTERGVVRIADVRIGDRVAIPEGGTTEVRAKR
                     RTRAKALRLALSDGTEVVTSYEHVFVLPDARQVEARTLTLGQPLLTRPIRRRAIDPVT
                     RLYTTALVQDARPVMVDGIEELGERDLIDISIEDRDELFLLANGVVTHNCKIGMGYHY
                     RARYECILFFEKGKRKLSDLGIADIIEASRISGGYPAEKPSAVSEVLIRQSTEPGQLV
                     IDPFMGSGSVGVAAVTAGRLFAGNDLCREAVDIARDRLREPGAQEIGSFGGLAGGAER
                     GARHGQLGLSGLADAPR"
     gene            8080..8559
                     /locus_tag="VF516_19515"
     CDS             8080..8559
                     /locus_tag="VF516_19515"
                     /inference="COORDINATES: protein motif:HMM:NF042873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease superfamily protein"
                     /protein_id="HEX8109933.1"
                     /translation="MGRQDRTEISGNGFRDLIAAYIHHQYSEHGLVVYREVNLGKTII
                     AKDRQIDVFVMRPVDQKAIAIECKYQDVQGTADEKIPYALDDLAALWVPGCLVYAGRG
                     WSKGVLHQLEASRLAAYCLPERPSLSRSKATRELDYILAATFGFWEQILPAAKRFRR"
     gene            complement(8585..>8879)
                     /locus_tag="VF516_19520"
     CDS             complement(8585..>8879)
                     /locus_tag="VF516_19520"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8109934.1"
                     /translation="ALCQGELIADDDLPPHVREKRPADFLGAAVARRMTLAELEREFI
                     ERVLEDEAGNKTRAAQRLGLDRKTLYRKLDEYARARGEVSPPAPAPAPGGEPE"
BASE COUNT         1485 a         2757 c         3097 g         1540 t
ORIGIN      
        1 ggcggcgaat cgccgcgacg gtcgggcggc ggcggcgcgc tgcgcagcga tctcgtccag
       61 cagcgcgtgc aaggtcggcc accggccgtc cggatccgcg gccaggccgc gggtcaacag
      121 gctggcaagc cacctcggcc ggtcgccgca agctggcggg accgggccag cggcgatcgc
      181 ggcgagcagc gcggcgacag ggtcggcgga acgatcggcc ggctctgcaa acggccgctg
      241 gccgtagacc gcttcccaca gggcgacgca aaagctgaat tgatcggcac gcgcatccac
      301 gtgctggccg cggagctgct cgggagacat gtaggcaggc gtgcccatga tctggccgct
      361 ctgggtcagc gtatctgcat gggcgaagct gatattcgca ttgacagcgc cgctgcgaac
      421 cgtgagctgc gcaaggccga aatccgcgac gaagacctcg ccgccgacgt ccagcaggac
      481 gttgtccggc ttgaagtcgc ggtgcacgat tcccatggaa tgggccgccg cgagaccgcg
      541 tcccgcagcg aggaacttgt cgaggatcgt atcgagcgag tgtcgctcgc ggttgaacca
      601 ttgccgcagc gtgccgccat cgagcaacgc catcgccagg aacacgccgc ctggagccag
      661 accgacgtcg aagatcggga ccacattggg atggcggagc tgtgccatcg tctgcgcttc
      721 ccgggacaag cgagcttggt ccgaatcgct tgcgccggcg cgtaccagct tgatcgcgac
      781 cgcgcgatcg agcttgggat cgtacgcgcg atagaccaca cccatggcgc ccgcaccgct
      841 catttcgcag atcacatagc gacccacgcg agctgggacg gggtgccgtg tcgcatcgct
      901 gaattcgtag ggactcgtcc gccttgcgtc gttcgacgtc gacatcccgg cttcagaata
      961 acggcctgcg tcgtatgtga gaagcaaaac cttggtgcag caaatggtgg cggtcccaat
     1021 gggacactgc tgatttcggc cctcctgaaa tcatcgaacc ggccacgtga ccggccgttc
     1081 gcatcgacgt tgcgaatcgc aacgaggatg agtgtctgcc atgttgcgtt ggtaagacac
     1141 actacgacga cgacatacct cgatgtgtga tacgtcacac gccggcctgg cggaggttgc
     1201 tatagcgatg gagattgtca tcgcgccatc gcttcaccta gagtgaacgc ggtggaaggc
     1261 gtggcagccg gtcggcatgt attggagatc gtcggcgtgc tggcgtcgga gaccgatgtc
     1321 gaggccgccg cccgtatcct gctggagcgg ctcctcgagg tgaccggcgc gcagcgcggc
     1381 tttatcgtcg tgcgcgaggc ggacagttac gtcgatgtat tcaacgtggc attcgaccgc
     1441 tcggcggtgt ccaccgagga gcgccggttc agccgccggc tggtccgcat ggctgctgcg
     1501 agccgcgagc cgctctactc ggcgtctgcc gccaccgatc cgcggttcgc ctcggtcgac
     1561 agcgtggcca gcctgggccc gcgggccgtg ctggtcgtcc cactgtgcga gcgcgaggta
     1621 gtgcacggca tcgtctactt cgactcgccg gtgccgatcg atgagccggc gcgtggctgg
     1681 gtgatcgagc tggcgcggtt gaccgcgccg ctgctgcgcc gggccgtggc cgaggaagcg
     1741 ctgcgccgcc gcgcgcgggc gctggagagc gacctgctgg cccagttcga cttcgagggc
     1801 atcgtcacgc gcgatcccca ggtgctggcg ctgctgcgta tcgcggccca ggtagcggat
     1861 gcgacggcga ctgtgctgat ccgcggcgag accgggaccg gcaaggagct cttggcccgc
     1921 gcgctgcatg tcaactcgtc gcgccgagag cggccgttcg tggcgctcca ctgcgccgcg
     1981 ctgcccgaca cggttctcga ggccgagctg ttcgggcacg tgcgcagcgc cttcaccggc
     2041 gccgaccgcg atcgcgcggg acggatcgcc agtgcgcgcg gtggcacgct gttcatcgac
     2101 gaggtgggag agatcccgct cggtgtccag gtcaagctgc tgcggtttct tcagtccggc
     2161 gagatccagc gcctcggcag cgatcgcccc gagcaggtcg aggtccgcgt ggtatgtgcg
     2221 accaatcgcg acctcgcggc gctggtgaag gaaggccggt tccgcgagga cctttactat
     2281 cgcatccagg tgatcgagct ggtcctcccg ccgctgcgcg agcggagcgg cgaccgtgag
     2341 ctgctggtgg agcacttcct cgcgcggttt gccaggcaaa agggcatggc gctgcggctg
     2401 accgcggagg cgcgcgcagc gctggtcacc taccactacc cgggcaacgc ccgcgagctg
     2461 gagcacgcga tcgagcgcat ggcgctgctg gcgaccaccc cggagctcgg cgtggagctg
     2521 ctgccaccgg cgatccggcc gggcggcccc gcgtcgacgt cgtttcgcga ctacaccagc
     2581 gacgagctgc gcgctgcgcg cgccgcggcc gtggcggagg tggaggcgcg cttcgtcgag
     2641 ggtctgctgc aaaaatccga tggcaatgtc tccgccgcgg cgcgcctggc cggcatgcca
     2701 cgcggctatc tgcagaagct gatcgcccgg aggcgtgggc gatgaccact ctgacgtgtc
     2761 gtcgctgccg gtcgctcaac ccgacctcaa cctgcggtgc aaccggcgcg gcggcccgtt
     2821 gcccgaggtc ttgaacgaca ccaccgcgcc ggcgctccac cagggtgtgg tcgaatccga
     2881 gctcgcatcg caggccgcga tgctggcggc gcgccggtgc gccaggcatc accaagatgc
     2941 gcgcggtcgt tcggacgctc gcggtcgccg ccccggggcg gtgcgcgcat gctcacgcgc
     3001 aagcctcgaa ggccgcagtc gagctcgcgc gctgaatgac ggaacgcggc gtacgccgag
     3061 ctgtcgggac tggcgatcga gccgcggagc gcgatccgga agcgccgaca tcgccgttgg
     3121 tctgccggcc gagccggtgg atcagccgaa aggcagggtg cctccgtgca gcggcagcga
     3181 agcggaccga acgcgcaggc ggcgccccgg cgatcgagtc ggtggtgagt gatttcaccg
     3241 gcatgcagga tcgaagcggg cgcaaggcgt ccttccccgc agggcatgca ccctgcatga
     3301 cctgctgaaa tgcgcgtgcg cgccgctcat gttgaacagt gtgcaagcgt tgactaacca
     3361 tggaggcttg gagatgagag ttctattgtc gatgcttatg atttgttgtg ttggatgtct
     3421 tccggatccc ggggatgagg atcttggcga tgcagaggaa gggggaactt ccacggtgga
     3481 ccaggccagc gtgtcggagg ggtttgtcgc gggagccggc gttgtaaccg acgacttcgg
     3541 tgatgagggc ccggtggaca tgagctcgca tcctgacagc ctggccacgg gcatgtggca
     3601 ggccatcctc tgggcggatg gtgccatcga gcagaacgat actcccttcg atacgtcgga
     3661 catcgattgt cagttcggac tgaacacggc ggcggccacc cggaactggc agagcgctca
     3721 cggggcgagc gtggatggct cagccggacc aatgacgctc ggccacgcgg atgaccgcct
     3781 cagcatctgt atcaatggct cgacgtccac tcagtctgta gtaagatatt ccggttcggt
     3841 gcatagccta ttctttattc gtaaggaagg ctcagggagt gtaaatcacg cctatcaagc
     3901 aatcggcacg gacggcaagt tgcgagtcat ctcctacggc ttcatctcgt cttgctcgag
     3961 tagcttcttc ttgggcttac ccagcagcgg ctctctgtgc tgcgatggta cctgtaagcc
     4021 aggttgcccc tgctgacgac gtgcatgtcc ggtggatgct ggctgcgctg atcgccggag
     4081 cggaccgcgg cgcgtggtcc atcaccgatg actgcggcgg cgcagaccgc gcaggctgac
     4141 gtcgaggcgg ctccggatca ggcggaggat gctgtccagc tcggtcgagc gcacgtcgat
     4201 ttcggcccgc gtgtgcgagc gggtcccagc cagcaggaac cggcgtgctc gttcgagcgt
     4261 ccgcgcacca gtcggacgtt cgcagtcgcc gcctagaacg ccacgttcac cagctgccag
     4321 gtggccggcg acgtccactg gtccgatcac tggccgctct acgcgaagat cacgctatga
     4381 ggtcaggaac cttgccgagc gctgccacaa aggcgcctgt aaagaatgga aggaggaagc
     4441 tatgctctca tttatgtatg tgcacacgat ttccgctatc atggccagac gcactttggc
     4501 gcgaatgtcg atggtcgcgc tgattgtcat cggctttacc accttgacaa cccccgcgat
     4561 cgcaagcgtc acgctgccag ggggcaaagc tcgattcgtt gtcgcagttg ggacgatacc
     4621 aacagttgat ggcagcggtt ccggctatag tgtgcgcttg gccgattata catttacggc
     4681 cggcagcacg ggtacggtca gtgaatcttt ttggtactgg agccaaggca ccatgagcgg
     4741 ccgatcggcc agcggtgtaa ccaactccgg ttgcgaatca cgggtttgtg atattggcgt
     4801 ccccacggga ttcgaagatc acacacggac agataccgct ccatacccga agagcttcac
     4861 tggcgcttat gcatacgacc cgtcaacctc atcggtcgtg attacatgga cagataattc
     4921 gatcgagcga tggacagttg actacggcaa tgacggattt cagccaatcg cgcgtatgca
     4981 catacgaagc acaaactatg gcgtacttga gggatgggca tatggtagca atgccgcatg
     5041 gacaacctcg gttgacatga catccatcgt tgctatgcat ccaagtttac atggcgatca
     5101 atatcagaac gactacggcg ccacggctat tttctcgctg gcctcgctag cgatacccgc
     5161 tactgcggaa ttatgcaata ctaattgctt ctctttcgag agccaggccg gcagtggtag
     5221 ctcgtgtgta tacacgggcg cggatggaaa acaacaccct ggttactacc gttggcggtt
     5281 cgctcatacg tcagattttc cgccacgcaa gaatgcatac aatatttact gtgaacagct
     5341 caatattcct caatcgcaac aagccatcac gcagcacgat gatggacaga catgttacaa
     5401 tggtaactat catatacgtc ccatgttgca gatcatcgat gatgatggat tgttccatgg
     5461 atgggtcggc gtggaagcct ctctctatca ggcgcaacag ggctccgcgt acgtacaggt
     5521 gttccacttc gatgacttgt aatggcacct cggcccgctt tctgtcagtg aatctggcct
     5581 tgacaccgca aggcgcgtcc gggcgcaagg ccgaggcgcg atcgcgcggc cgaccgcgag
     5641 ctggggctgc tcatcagcca aggccgtgtc ggcgacctcg accgccaggg gcgaaccagg
     5701 cgcggcagtt gggtcggcaa ccggacgccg cggttctccg ctgcgagcgc tgcgaggccg
     5761 gctggctggt tgccgatcgc ggcctcgggg cagactcggg tggcggctga cgcagggatc
     5821 agatcagcag tgtcggctga ccggacgcgg cttgacgccg acgccgcagc gaagcgccat
     5881 cgcgacgccc tcggccttgc gggggtcgcg tccccttgat cctttcccac agcgcgcgct
     5941 cgatccgctc ggcgcttgcc tgcaggtgat cgagtcgttc ggtctggacg tagtgcttga
     6001 cgttgacgcc gcgcttcgag tgattcatca gcgcctcgcg cgtctccaga tcgatcccga
     6061 tctccgcggc caccgagttg aaggtgcgac gcgacacatg gatcccgggc aacgtgttca
     6121 cgcgcactgg ttcgccgtcc gcatcgcgca tgacccggcc gttggcgttc gtaagctgat
     6181 ggcgttcctt cacctcggcg accgggatca ctcgtttcat gtcgcgcgac agggaaggga
     6241 agacgaagcg tgatcgcctc cgaaccgcgc cagcagcggg ctcgtggcgt tgtcagcctt
     6301 gcgccgctgg aggatcttgc gaacggtctt gaccatcggc agggagtagg ggcgatcgcc
     6361 ctttgtgcgc gccacctgga tcagctccgc gtcaaagtca acgtcctccc agcgcaggtt
     6421 gcgcacgccg tcgctccgga taccggtgaa cagggcgacc agatgcagat cgcgccgcac
     6481 cgggttctcc agcttcagga ccttggcgta cccgtccgcg aactcggtat ccgggatccg
     6541 tgtctcacgc ggcttgagtg ccgcctgttg caggcgcgtg gctgggttca gcgcgcgatc
     6601 gatcaaaccg atcgatgctc taggcagcgg cggcgatgca aaccggctaa ggccagcgct
     6661 ggcgaggagg tcgtcgatct tcgcggccga gtgcagtgcg ggtggacgtc ctggacggtc
     6721 ctcctgagcc ggactcggtg agggtgaggg ctcgcacagc gggcgagatc tccgtcggca
     6781 ctgaacggat tgtcagaccg gcgtgcgagg gtaggcgcat ggacgggcct gccgtgtctt
     6841 tgaagtttca cctcgcgcag agcgacgcgg tggcgtggct gcgcacgctg ccgtccgagt
     6901 cggtggacct ggtggtcacc gacccgccgt acgagtcgct ggagaagcac cgcgcgatcg
     6961 gcaccaccac gcggctcaag cacagcaagg cgtcgagcaa cgactggttc gcgatcttcc
     7021 ccaacgagcg gtttcccgag ctgttcgccg aggtgtaccg cgtgctgcgg cgcaacacgc
     7081 acttctacct gttctgcgat cccgagacga tgttcgtggc caagccggcg ggggagcagg
     7141 cggggttcaa gttctggaag ccgctgatct gggacaagtg cctgggaccg gacacgcggc
     7201 tatggaccga gcgcggggtg gtgcggatcg ccgacgtgcg gatcggggat cgcgtggcga
     7261 tcccggaggg cgggacgacg gaggtgcggg ccaagcggcg gacgcgggcg aaggcgctgc
     7321 gcctggcgct gtcggacggg accgaggtcg tgacgtcgta cgagcacgtg ttcgtgctgc
     7381 cggatgcgcg gcaggtcgag gcgcgcacgc tgacgctcgg tcagccgctg ctgacgcggc
     7441 cgatccggcg gcgcgcgatc gatccggtga cccggctgta cacgacggcg ctggtgcagg
     7501 acgcgcggcc ggtcatggtg gacgggatcg aggagctcgg cgagcgcgat ctgatcgata
     7561 tctcgatcga ggatcgcgat gagctgttcc tgctcgccaa cggcgtggtc acgcacaact
     7621 gcaagatcgg catgggctac cactaccggg cgcgctacga gtgcatcctg ttcttcgaga
     7681 agggcaagcg caagctgagc gacctcggca tcgcggacat catcgaggca tcccggatct
     7741 cgggcggcta tccggcggag aagccgtcgg cggtgagcga ggtgctgatc cggcagagca
     7801 cggagcccgg gcagctggtg atcgatccgt tcatgggctc ggggtcggtg ggggtcgcgg
     7861 cggtgaccgc cggccggctg ttcgccggca acgacctgtg ccgcgaagcg gtcgacatcg
     7921 cgcgcgaccg gctgcgcgag cccggggcgc aggagatcgg gagcttcggc gggctggccg
     7981 gcggcgcgga gcgaggcgcg cggcacgggc agctcgggtt gtcggggctg gcggatgcgc
     8041 cgaggtagcg cgcggcgggc gagggacccg aggtaagcca tggggcgcca ggatcggacc
     8101 gagatcagcg gcaatggctt tcgcgatctg atcgcggcct atatccatca ccagtacagc
     8161 gaacatggtc tggtggtgta tcgcgaggtc aacctcggca agacgatcat cgccaaggac
     8221 cgccagatcg acgtgttcgt gatgcggccc gtggatcaga aggcgatcgc gatcgagtgc
     8281 aagtatcagg acgtccaggg caccgctgac gagaagatcc cgtacgcgct cgacgacctc
     8341 gcggcgctgt gggtgccggg ctgcctggtc tacgccggcc gcggctggtc caagggcgtg
     8401 ctgcaccagc tggaggcgtc gcggctggcg gcgtactgcc tgccggagcg gccgagcctg
     8461 tcgcgctcga aggcgacccg cgagctcgac tacatcctgg cggcgacgtt cgggttctgg
     8521 gagcagatcc tgccggcggc caagcggttc cggaggtgag gccgtggtgc ggccatgccg
     8581 ggcgctactc gggctcgccg ccgggggccg gggcaggcgc cgggggggag acctcgccgc
     8641 gcgccctggc gtactcatcg agcttgcggt acagggtctt gcggtcgagg ccgagccgct
     8701 gggcggcgcg ggtcttgttg ccggcctcgt cctcgaggac gcgctcgatg aactcgcgct
     8761 cgagctcggc cagggtcatc cggcgcgcga ccgcggcgcc gaggaagtcg gccgggcgct
     8821 tctcgcggac gtgcggcggc aggtcgtcgt cggcgatcag ctcgccctgg cacagcgcg
//
LOCUS       DASVOR010001006         1874 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_243967,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001006 DASVOR010000000
VERSION     DASVOR010001006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1874
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243967"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VF516_19525"
     CDS             complement(<1..428)
                     /locus_tag="VF516_19525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109935.1"
                     /translation="MHLERSFRGSDESSAFDWERPAQDLDIPHDVARALYRRALQSAD
                     DLRRAEAIYRRWLQDVVAARTPVSPPPAPGRQTRVEREARGRRPRPQDAAVGLGKWTR
                     SLLETEVDDAAPGADEVHRAIAALSAGTVLPGPADEAAPAA"
     gene            1158..>1874
                     /locus_tag="VF516_19530"
     CDS             1158..>1874
                     /locus_tag="VF516_19530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109936.1"
                     /translation="MTNATSPPSLQTFTLADLQQFTKEGRYASHASKDFHLFYAGRDD
                     VHGILKYLLSRARVSLYLNMYGYDDDELNDECMRCAGDPSITTLITLDESQARGRHEK
                     QILDSDMARNPTAFNTHVIVIGRSATQQISHTKGGVLDGQVGFEGSTNWSVSGEGTFV
                     LKGKPGGPGYKAQNNTLAVFTDPDAISRFTAELIAEHMAATGGGLASTALAGTPGGTS
                     PGALTPARGRVRGAAMRGAGS"
BASE COUNT          322 a          622 c          635 g          295 t
ORIGIN      
        1 gccgccggcg cggcttcgtc ggccgggccg gggagcacgg tgcccgcgct cagcgcggcg
       61 atcgcgcgat ggacctcgtc ggcaccgggg gcggcgtcat cgacctcggt ctccagcagc
      121 gagcgcgtcc acttcccgag accgaccgcg gcatcctggg gccgagggcg ccgccctcgc
      181 gcttcgcgct cgacgcgggt ctggcggccc ggcgcgggtg gtggcgagac cggcgtccgc
      241 gccgccacca cgtcctggag ccatcgccgg tagatcgcct cggcccgacg cagatcgtcc
      301 gcgctctgga gggcgcggcg gtacagcgcg cgcgcgacgt cgtgcgggat gtccagatcc
      361 tgcgccggac gctcccagtc gaacgccgac gactcgtcgc tgccccgaaa cgaccgctct
      421 agatgcatca cgctcctgca acgtcccctc ccctcagagg gagacgcgcc aacatactac
      481 ggggctggca gcggattcct tacctgcgtt gcgatgcggc gccgcgagca cgcagatccg
      541 gacggcaggc gttgcctccg gatgccggga aagcgttgtg aatgcaggtc gatgcgccgc
      601 caagcggccg accccgcgac gtcgctccac gcccggaagg gcgccgggga acacggtctt
      661 ccggcgggtc ggcgctaccc aggtgcgtta ttatcgtact tgatcttaca taagcacagg
      721 tggaggagca cctcggagtt gcagccgctc cggcacacgg ccggcggcgg cgcggccacc
      781 gagccatggg cggtcggagc ggccaccgag ccatgggtcc tcgagcggga ggtgccatgc
      841 gcgcgcgtga tcgccggtcg cgatcaacgc acgatggaga tgcagcgcat cggtctcgcg
      901 atcgctccgg tagctggcac gcaggtcgtt gcctgccgca tcgcagacag cgcccccctc
      961 gtccggcagg atccggcagt cttgcgcgag agccaggcgc aacgcgtgcc ccatcgtaga
     1021 tggcgccatg tgaactcatg taatcatccg gcggccggtg actgcgatgt gctcatgatc
     1081 ggcagtggtc cgtcgagaag aattagcaat gaatcgtctt gccgctccat ctcctggcgt
     1141 caaggtgaaa tacagcgatg acgaacgcga ccagcccgcc gagcctgcag acgttcacgc
     1201 tggccgacct tcagcagttc acgaaagaag gtaggtatgc atcgcacgcg agcaaggact
     1261 ttcacctgtt ctatgccggt cgggatgatg tccacgggat tctcaaatac ttgctctcgc
     1321 gtgcgcgcgt gtcgctctac ttgaacatgt acggctacga cgatgacgag ctgaatgatg
     1381 aatgcatgcg atgtgcgggc gatccgagca tcacgaccct gatcacgctc gatgagagcc
     1441 aggcccgggg gcgacacgaa aagcagattc ttgattccga catggccagg aatcccacgg
     1501 cattcaatac gcacgtgatc gtgatcggcc gatccgcaac acagcagatc tcccatacca
     1561 agggtggcgt gctcgatggt caggtcggct tcgaggggtc cacgaactgg agcgtatcgg
     1621 gcgagggcac gttcgtgctg aagggcaagc cgggcggacc gggttacaag gcacagaaca
     1681 acacgctcgc cgtgttcacg gatcccgatg cgatctctcg cttcacggcc gagctcattg
     1741 ccgaacacat ggcagccacc ggcggcgggc tggcctcgac ggctctggcc ggcacgccgg
     1801 gcggcacatc cccgggagcc ctgactccgg cgcgaggtcg ggttcgcggc gcggcgatgc
     1861 gtggcgctgg ctcg
//
LOCUS       DASVOR010001007         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_98323,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001007 DASVOR010000000
VERSION     DASVOR010001007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98323"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..355)
                     /locus_tag="VF516_19535"
     CDS             complement(<1..355)
                     /locus_tag="VF516_19535"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); Derived by automated computational
                     analysis using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEX8109937.1"
                     /translation="MTNAKAETAITPTRAENYADWYLEVIKAADMAEPSTVRGCMVIK
                     PWGYALWEHIQRGLDGMFKATGHVNAYFPLFIPVSLLEKEAAHVEGFAKECAVVTHHR
                     LEARDGKLVPTGELEE"
     gene            complement(461..1120)
                     /gene="tsf"
                     /locus_tag="VF516_19540"
     CDS             complement(461..1120)
                     /gene="tsf"
                     /locus_tag="VF516_19540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEX8109938.1"
                     /translation="MAEVTATLIKDLRERTGAGMADCKKALTEVGGDMDKAIDYLRTK
                     GLAKAAKKAGREATEGAVVSYIHGGGRIGVLVEVNCETDFVARNEDFQGFTREVALQI
                     AAMNPQFVRRDEVSQDVIDREREVLVAKAKESGKPDPVVQKMVDGQITKWLKEICLLD
                     QTWVKDPDKTIDQVQQELIAKIGENIKVRRFVRFELGEGLEKKKDDFAAEVAKQAGLA
                     H"
     gene            complement(1124..>1877)
                     /gene="rpsB"
                     /locus_tag="VF516_19545"
     CDS             complement(1124..>1877)
                     /gene="rpsB"
                     /locus_tag="VF516_19545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEX8109939.1"
                     /translation="YIFGARNGIHIIDLQHTVKLFRQAFQAVVDTTGRGETILFVGTK
                     KQAQDVIVEEATRSNQHYVTQRWLGGTLTNFRTVKGSLERLRGLEKMEEDGTLFALTK
                     REQVMIKRDRDKLMKSLGGIKGLSKLPGMLFVIDPHKEHIAVDEARKLEIPVVAITDT
                     NCDPDQIDYVIPGNDDAIRAIKLFASKIGDAAILGTKIWKETARNRGQEREETGERVI
                     HVSSGGDGPRVEMSTGGSMFNPEASASPDLEP"
BASE COUNT          255 a          647 c          616 g          359 t
ORIGIN      
        1 gctcctcgag ctcgccggtc ggcaccagct tgccgtccct ggcctcgagc cggtggtggg
       61 tgaccacggc gcactccttg gcgaagccct cgacgtgcgc ggcctccttc tcgagcagcg
      121 acaccgggat gaacagcggg aagtacgcgt tgacgtggcc ggtggccttg aacatgccgt
      181 cgaggccgcg ctggatgtgc tcccacagcg cgtagcccca cggcttgatc accatgcagc
      241 cgcgcaccgt cgacggctcc gccatgtcgg cggccttgat cacctcgagg taccagtcgg
      301 catagttctc cgcgcgcgtc ggcgtgatcg cggtctcggc cttggcgttg gtcatggccg
      361 cgaagatgta cctcagcgcc cgggcggccg ccacgggcag cgatcagcga atgacagcga
      421 cggggctgcg aacgacgctg cgaacgacgc tggaacgacg ctagtgcgcg agcccggcct
      481 gcttggcgac ctcggcggcg aagtcgtcct tcttcttctc gaggccctcg ccgagctcga
      541 accgcacgaa ccggcggacc ttgatgttct cgccgatctt ggcgatcagc tcctgctgga
      601 cctggtcgat cgtcttgtcg gggtccttga cccaggtctg atcgagcaga cagatctcct
      661 tgagccactt ggtgatctgg ccgtcgacca tcttctgcac gacggggtcc ggcttgccgc
      721 tctccttggc cttggcgacc aggacctcgc gctcgcggtc gatcacgtcc tgcgagacct
      781 cgtcgcggcg gacgaactgc gggttcatcg ccgcgatctg cagcgcgacc tcgcgggtga
      841 acccctggaa gtcctcgttg cgggcgacga agtcggtctc gcagttgacc tcgaccagca
      901 caccgatccg gccgccgccg tggatgtacg acaccaccgc gccctcggtc gcctcgcggc
      961 cggccttctt ggcggccttg gcgaggccct tggtgcgcag gtagtcgatc gccttgtcca
     1021 tgtcgccgcc gacctcggtc agcgccttct tgcaatccgc catgcccgcg ccggtgcgct
     1081 cgcgcaggtc cttgatcaac gtcgcggtga cctcggccat gactcagggc tccagatcgg
     1141 gagacgcaga cgcctccggg ttgaacatgc tgccgccggt cgacatctcg acccgcgggc
     1201 cgtccccgcc cgacgacacg tggatcacgc gctcgccggt ctcctcgcgc tcctggccgc
     1261 ggttgcgcgc ggtctccttc cagatcttgg tgccgaggat cgcggcgtcg ccgatcttcg
     1321 acgcgaacag cttgatcgcg cggatcgcgt cgtcgttgcc cgggatcacg tagtcgatct
     1381 ggtcggggtc gcagttggtg tcggtgatcg cgaccaccgg gatctccagc ttgcgggcct
     1441 cgtcgaccgc gatgtgctcc ttgtgcggat cgatcacgaa cagcatgccg gggagcttcg
     1501 acaggccctt gatgccgccg agcgacttca tcagcttgtc gcggtcgcgc ttgatcatca
     1561 cctgctcgcg cttggtcagc gcgaacagcg tgccgtcctc ttccatcttc tcgaggccgc
     1621 gcagccgctc gagcgagccc ttgacggtgc ggaagttggt cagcgtgccg ccgagccagc
     1681 gctgggtgac gtagtgctgg ttcgagcggg tcgcttcctc gacgatcacg tcctgcgcct
     1741 gcttcttggt gccgacgaac aggatggtct cgccgcgccc ggtggtgtcg accaccgcct
     1801 ggaacgcctg gcggaacagc ttgaccgtgt gctggaggtc gatgatgtgg atgccgttgc
     1861 gcgcgccgaa gatgtac
//
LOCUS       DASVOR010001008         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156323,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001008 DASVOR010000000
VERSION     DASVOR010001008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156323"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2503)
                     /locus_tag="VF516_19550"
     CDS             complement(<1..>2503)
                     /locus_tag="VF516_19550"
                     /inference="COORDINATES: protein motif:HMM:NF023945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8109940.1"
                     /translation="RRQLPSFVYLASDIDLAPHETALPWRPDAPATALRVIVGELARS
                     QGARMASRMIASAKSWLCHPGVDRQAAILPWGGDDQKISPVEAQALILGHVRDAWDHA
                     HPGAPLAEQEVLVTVPASFDEAARELTLQAAAKAGYPPIVLLEEPQAAFYAWVDSRSG
                     GADQRLAAGDRVLVFDIGGGTTDFTLIDVTDDGFTRTAVGDHLLLGGDNLDLTLAKLI
                     EQRLVERAGKKLDALQWHGLVHACRLAKETLLGDDPPAAAPIVVQGRGARLIAGTLRD
                     EITRAELDAVLFDGFFPQVAVDAPLARGRGGLQEFGRPSAADPAVTRHLAAFLSRHGR
                     PRISAVLFNGGAMTPRSLRVRVIDQIAAWQDAAPRELPAAAPELAVAQGAAYYGLVRR
                     GQAIRIRGGTPRAFYIGVDAGAAHMAVCLAPSGLDDGARVQLARDFRMVTNRPVSFRL
                     YSSSSRSDPPGALVPIGDGRPETVGEGDSDLLELPPIVTVLRARGRGEVTVRLEVHIT
                     ELGALEISCVEVAAEPGATPETWKLAFDMRSGGSAAPPAATAEAHPRSEDARARIQRA
                     FQTGDGLATLTRDLEAMLDARRDDWSVATARALFDALVEVAAERKKTPDHEQRWLNLA
                     GFLLRPGTGAPLDAWRTRVLWGVFNENLAHPRSEPGKLAWWITWRRIAGGLAKGQQDQ
                     IFDRLARLLLPNPLQAKKLTEARPSKQELAEMWRVVASLERIPVGHKLKLGDELMRRL
                     EPRKGREDPVHLWALSRLGARVPLYGPLNAVIAPGKIADWLTPLCAWDWPEPDKAAFP
                     LAQLGRRTGDRTRDLDDPSRAQLATTVRALPGGERA"
BASE COUNT          309 a          856 c         1027 g          311 t
ORIGIN      
        1 gcgcgctcgc cgccggggag ggcgcggacg gtggtggcga gctgggcgcg agaggggtcg
       61 tcgagatcgc gggtgcggtc gccggtgcgg cggccgagct gggcgagcgg gaatgcggcc
      121 ttgtcgggtt ccggccagtc ccacgcgcac agcggggtca gccagtcggc gatcttgccg
      181 ggggcgatca cggcgttgag cgggccgtag agcgggacgc gcgcgccgag ccgcgacagc
      241 gcccacaggt ggaccggatc ctcgcggccc ttgcggggct cgagccggcg catcagctcg
      301 tcgccgagct tgagcttgtg gccgaccggg atgcgctcca ggctggccac gacgcgccac
      361 atctcggcga gctcctgctt gctcggccgg gcctcggtca gcttcttggc ctgcagcggg
      421 ttggggagca agagcctggc gagccggtcg aagatctggt cctgctggcc cttggcgagg
      481 ccgccggcga tccgccgcca ggtgatccac caggcgagct tgccgggctc gctgcgcggg
      541 tgggcgaggt tctcgttgaa cacgccccac agcacgcggg tgcgccaggc gtcgagcggg
      601 gcgccggtgc cggggcggag caggaagccg gcgaggttga gccagcgctg ctcgtggtcg
      661 ggcgtcttct tgcgctcggc ggcgacctcg acgagggcgt cgaacagggc gcgcgcggtg
      721 gcgaccgacc agtcgtcgcg gcgcgcgtcg agcatggcct cgagatcgcg ggtcagggtg
      781 gcgaggccgt cgccggtctg gaacgcgcgc tggatccgcg cgcgggcgtc ctcgctgcgc
      841 gggtgggcct cggcggtcgc ggcggggggc gccgcgctgc cgcccgagcg catgtcgaac
      901 gcgagcttcc aggtctcggg ggtggcgccg ggctcggcgg cgacctcgac gcacgagatc
      961 tcgagcgcgc cgagctcggt gatgtggacc tcgaggcgga cggtgacctc gccgcggccg
     1021 cgcgcgcgca gcacggtgac gatcggcggc agctcgagca ggtccgaatc accttcgccc
     1081 acggtctcgg gccggccgtc gccgatcggc accagggcgc cgggcgggtc gctgcgcgag
     1141 ctcgacgagt acagccggaa gctgaccggc cggttggtga ccatgcggaa gtcgcgcgcg
     1201 agctggaccc gcgcgccgtc gtcgaggccc gagggcgcga ggcacaccgc catgtgcgcg
     1261 gcgccggcgt cgaccccgat gtagaacgcg cgcggggtgc cgccccggat ccggatcgcc
     1321 tggccgcggc ggaccaggcc gtagtaggcg gcgccctggg cgaccgcgag ctcgggcgcg
     1381 gcggcgggca gctcacgcgg cgcggcgtcc tgccaggcgg cgatctggtc gatcacgcgg
     1441 acgcgcagcg agcgcggcgt catcgcgccg ccgttgaaca gcacggccga gatccggggg
     1501 cggccgtggc gcgacaggaa cgccgcgagg tggcgggtca ccgccgggtc ggccgcggag
     1561 ggccggccga actcctggag gccgccccgg ccgcgcgcga gcggggcatc gacggcgacc
     1621 tgggggaaga acccgtcgaa cagcacggcg tcgagctcgg cgcgggtgat ctcgtcgcgc
     1681 agcgtgccgg cgatcagccg ggcgccgcgg ccctgcacga cgatcggggc cgcggccggc
     1741 ggatcgtcgc cgagcagggt ctccttggcg agccggcagg cgtggaccag gccgtgccac
     1801 tgcaaggcgt cgagcttctt gccggctcgc tcgaccagcc gctgctcgat gagcttggcg
     1861 agcgtgaggt cgaggttgtc gccgccgagc agcaggtgat cgccgaccgc ggtgcgcgtg
     1921 aagccgtcgt cggtgacgtc gatcagcgtg aagtcggtgg tgccgccgcc gatgtcgaac
     1981 accaggacgc gatcgccggc ggcgagccgc tggtcggcac cgccgcttcg cgagtcgacc
     2041 cacgcataga acgcggcctg gggctcctcg agcaggacga tcggcgggta gccggccttg
     2101 gcggccgcct gcagggtgag ctcgcgcgcg gcctcgtcga acgaggcggg cacggtgacc
     2161 agcacctcct gctcggcgag cggcgcgccg ggatgcgcgt ggtcccacgc gtcgcggacg
     2221 tggccgagga tcagcgcctg ggcctcgacc ggcgagatct tctggtcgtc gccgccccac
     2281 ggcaggatcg cggcctggcg gtcgacgccg gggtggcaca gccacgactt ggccgacgcg
     2341 atcatccgcg acgccatgcg ggcgccctgg ctgcgcgcca gctcgccgac gatcacgcgt
     2401 agtgcggtcg cgggggcatc cggtcgccac ggcagcgcgg tctcgtgcgg cgcgagatcg
     2461 atatcgctcg cgagatagac gaacgacggc agctggcggc gcg
//
LOCUS       DASVOR010001009         1561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156395,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001009 DASVOR010000000
VERSION     DASVOR010001009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1561
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156395"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1519)
                     /locus_tag="VF516_19555"
     CDS             complement(<1..1519)
                     /locus_tag="VF516_19555"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8109941.1"
                     /translation="MSQLFARVNVDSQSASGTSGELRRVLGKWDLTALGVGAIIGAGI
                     FASTGSAIAGGADHVGAGPAIVLSFVLTAIACGFAALCYAEFAAMVPVSGSAYTYAYA
                     ALGELVAWIIGWDLIIEYAIGNVAVAVSWSGYFCGLLELFGIHVPTWLATDYRSAHQA
                     ADLIAAGQGTPDLAPVAAALHDAPRIAGIPIIFNLPAVVIVAVITWILVRGVKESARS
                     NFWMVVLKLAIIVAFLVVGAFYVQPENWTVHGGFAPNGFDGIGSAAAIIFFAYIGFDA
                     VSTASEEAKDAKRDMPFGIIASLVICTVLYIVVALVLTGAAPWTEVGTAEPMLTVLER
                     TGGQGFALKAARLFVALGAVIAMSSVLLVFQLGQPRIFFSMARDGLLPPWAARIHPRY
                     KTPHITTIITGVFVGVTSAFVNINEAVELTNIGTLFAFVLVAIGVIVLRVREPDRPRP
                     FRVPWSPVTPVLAVLSCLYLMSKLPLATWIRFAAWLIIGLALYFLYGFEHSKLRKK"
BASE COUNT          288 a          512 c          567 g          194 t
ORIGIN      
        1 atttcttgcg gagcttcgag tgttcgaagc catacaggaa gtacagcgcg aggccgatga
       61 tgagccaggc ggcgaaccgg atccacgtgg ccagcggcag cttggacatc aggtagaggc
      121 aagacagcac tgcgagcacc ggcgtgaccg gcgaccaggg cacgcggaac ggccgggggc
      181 ggtcgggctc gcggacgcgc agcacgatga cgccgatcgc gaccagcacg aacgcgaaca
      241 gcgtgccgat gttggtgagc tcgacggcct cgttgatgtt cacgaacgcc gaggtgacgc
      301 cgacgaacac gccggtgatg atcgtcgtga tatggggcgt cttgtaccgg gggtggatcc
      361 tggcggccca cggcggcagc aggccgtcgc gcgccatcga gaagaagatc cgcggctggc
      421 cgagctggaa caccaggagc accgagctca tcgcgatcac cgcgccgagc gcgacgaaca
      481 ggcgcgcggc cttcagcgcg aagccctggc cgccggtgcg ctccagcacg gtgagcatcg
      541 gctcggccgt gccgacctcg gtccacggcg cggcgccggt gagcacgagc gcgacgacga
      601 tgtagagcac ggtgcagatc accaggctcg cgatgatgcc gaacggcatg tcgcgcttgg
      661 cgtccttcgc ttcctcggag gcggtcgaca ccgcgtcgaa gccgatgtac gcgaagaaga
      721 tgatcgcggc cgcgctcccg atcccgtcga agccgttggg cgcgaagccg ccgtgcaccg
      781 tccagttctc gggctggacg tagaacgccc cgacgaccag gaacgcgacg atgatcgcga
      841 gcttgagcac gaccatccag aagttcgagc gcgccgactc cttgaccccg cggaccagga
      901 tccacgtgat caccgcgacg atcaccaccg cgggcaggtt gaagatgatc gggatcccgg
      961 cgatgcgggg cgcgtcgtgc agcgccgcgg cgaccggtgc gaggtcgggc gtgccctggc
     1021 cggcggcgat caggtcggcg gcctggtgcg cagagcggta gtcggtggcg agccaggtcg
     1081 gcacgtggat gccgaacagc tcgagcaggc cgcagaagta ccccgaccac gagaccgcga
     1141 cggcgacgtt gccgatcgca tactcgatga tcagatccca gccgatgatc caggcgacga
     1201 gctcgccgag cgcggcgtag gcgtaggtgt aggccgagcc ggacaccggc accatcgcgg
     1261 cgaactcggc gtagcacagc gcggcgaagc cgcaggcgat cgcggtgagg acgaacgaga
     1321 ggacgatcgc gggtccagcc ccgacgtggt cggcgccacc ggcgatcgcc gagccggtcg
     1381 acgcgaagat gcccgcgccg atgatcgcgc cgaccccgag cgcagtgaga tcccacttgc
     1441 ccagcacgcg gcggagctcg ccggaggtcc cggaggcgct ctgtgagtcg acgttgaccc
     1501 gggcgaacag ctggctcatg acgcgttcat aacacgccgg tcgcgcccga cgcgcggccg
     1561 c
//
LOCUS       DASVOR010001010         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_331475,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001010 DASVOR010000000
VERSION     DASVOR010001010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331475"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            165..1448
                     /locus_tag="VF516_19560"
     CDS             165..1448
                     /locus_tag="VF516_19560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX8109942.1"
                     /translation="MTAGRTVVIGAGVSGLSFANWWRERHPGADVVVLEAEAEPGGYC
                     RTVVQDGFVWDYSGHFFHFRDPAIEAWLRARMPGQEVRTVVRKSAIRHAGRDIDFPFQ
                     KHIHQLPLDEFLDCLVELYFRPTGDAPPRSFGEMLYRKFGKGITERFLRPYNEKLYAT
                     DLDRLDVDAMGRFFPHADIADIIASMRPRPPGAPAEGYNATFTYPAGGAVQYIHALLR
                     DLPPDAVRCHERVKAIQIGDRCVQARGRTRCDRVVSSLPLPQLARMCEIDHDPSVFSS
                     NKVAVYNLGFDRKGQRDIHWMYFPDPSIVFYRVGWYDNILPPAPGAPDRMSLYVEIGV
                     PSDAELHEAGTFERVMEDLRREGIVTDHQLVSYHHVMLDPAYVHITQASIAEAARIKA
                     ELARYNIYSVGRYGGWTYCSIEDNMLETRALAQQL"
     gene            complement(1522..2100)
                     /locus_tag="VF516_19565"
     CDS             complement(1522..2100)
                     /locus_tag="VF516_19565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8109943.1"
                     /translation="MTARGDVDKDPRGIFICVAANTTAMHGWCVDFRAWIAHPRHDEV
                     PCMRVTSPSTPGRRVLIVDDHPDSADASCLLLTLLGYEAWPATSGAQALAEVERLHPD
                     VVICDLGLPDINGYEVARKIRSRHGAALYLVALTGWDQLADRERALAAGFDQHLVKPA
                     NTAHLQAVMAASTRRGDSAPRGDGKRRALHRP"
BASE COUNT          363 a          790 c          755 g          361 t
ORIGIN      
        1 acctcgttcg agctcggcgt gatccactac gcgtcggaca ccccgtactc gcggcgccag
       61 agcctcttgt ccaagagcct gtcgtacttc ggcatggaga gcacctacgg cgccgcggcg
      121 gcgatcgcgc agcggatctt ccggaacgag ctcgcgaggc tggagtgacc gccggccgca
      181 ccgtcgtcat cggggccggc gtctcggggc tgtcgttcgc caactggtgg cgcgagcgcc
      241 accccggggc cgacgtcgtc gtgctggagg cagaagccga gcccggcggt tactgccgta
      301 cggtggtaca ggacggcttc gtgtgggact actcgggcca cttcttccac ttccgggacc
      361 cggcgatcga ggcctggctg cgcgcccgga tgccgggcca ggaggttcgc accgtcgtcc
      421 gcaagagcgc gatccgccac gccggccgcg acatcgactt cccgttccag aagcacatcc
      481 accagctgcc gctcgacgag ttcctcgact gcctggtcga gctgtacttc cggcccaccg
      541 gcgacgcccc gccgcgctcg ttcggcgaga tgctctaccg caagttcggc aagggcatca
      601 ccgagcgctt cctccggccg tacaacgaga agctctacgc caccgacctc gaccggctcg
      661 acgtcgacgc gatgggccgg ttcttcccgc acgccgacat cgccgacatc atcgccagca
      721 tgcggccgcg cccgccgggc gcccccgccg agggctacaa cgccaccttc acgtaccccg
      781 ccggcggcgc ggtccagtac atccacgcgc tgctccgcga cctgccgccg gatgcggtgc
      841 gctgccacga gcgggtcaag gcgatccaga tcggggatcg ctgcgtgcag gcccgcgggc
      901 ggacccgctg cgatcgcgtg gtgtcatcgc tgccgctgcc ccagctcgcg cggatgtgcg
      961 agatcgacca cgatccgtcg gtattctcgt cgaacaaggt cgcggtgtac aacctcggct
     1021 tcgaccgcaa gggccagcgc gatatccact ggatgtactt tccggatccg tcgatcgtgt
     1081 tctaccgggt cggctggtac gacaacatcc tgccgcccgc tcccggggcg cccgaccgga
     1141 tgagcctcta cgtcgagatc ggcgtgccga gcgacgcgga gctgcacgag gccggaacgt
     1201 tcgagcgggt gatggaggac ctgcggcgcg agggcatcgt caccgaccac cagctcgtct
     1261 cgtaccacca cgtcatgctc gatccggcgt acgtccacat cacgcaggcc tcgatcgccg
     1321 aggctgcgcg gatcaaggcc gagctcgcca ggtacaacat ctactcggtg ggccgctacg
     1381 gcggctggac ctactgctcg atcgaggaca acatgctcga gacccgcgcg ctggcgcagc
     1441 agctgtgagc tgccgggtcg cggacgcgcc gtgacggctc gccgtgacag catgggccca
     1501 tgatggggcg ccggtggttc gtcatggacg atggagagca cgccgcttcc catcgccccg
     1561 gggtgcgctg tcaccacgtc gcgtcgatgc ggccatgacc gcctgcaagt gcgcggtatt
     1621 ggccggcttg acgaggtgct gatcgaaccc ggcggcgagc gcgcgctcgc gatccgcgag
     1681 ctgatcccag ccggtgagcg ctacgaggta cagcgcggca ccgtgccgac tccggatctt
     1741 gcgcgcaacc tcgtagccgt tgatatccgg caggccgagg tcgcaaatca caacgtcggg
     1801 atgtaaccgc tccacctcgg cgagtgcttg cgcgccgctg gtcgctggcc acgcttcgta
     1861 cccgaggagg gtcagcaaca ggcaggatgc gtcggccgag tccgggtgat catcgacgat
     1921 caagacccgg cggccgggag tgctcggcga tgtgacgcgc atgcacggca cttcatcatg
     1981 acgtggatgc gcgatccaag cacgaaagtc gacacaccac ccatgcatcg cggtggtgtt
     2041 ggcagcgaca cagatgaaaa tcccacgcgg gtccttatcc acatcccccc gcgcggtcac
     2101 gccacacagg cggcgggcgg ctcgatcgcg gtcagtgctg gtactccatg cgcacgtagt
     2161 agaacgcgcc gtcggtgccg aacggcgtgc cggcggggac cgacgccggg ctgtacagga
     2221 agctgttgtt gtagcggttg tcctcgtgct tcatctgatc ggggaaggt
//
LOCUS       DASVOR010001011         8229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_536231,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001011 DASVOR010000000
VERSION     DASVOR010001011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8229
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536231"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1281..1952)
                     /locus_tag="VF516_19570"
     CDS             complement(1281..1952)
                     /locus_tag="VF516_19570"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - obsolete oxidoreductase
                     activity, acting on NAD(P)H, nitrogenous group as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEX8109944.1"
                     /translation="MSLDVLSAMTARHCKRAYLDRPVPQALLRQVLGAAAHAPSTRNA
                     QPWQVVVLSGAARMRLADRLCAAFDADEPARPDYVNRRPTTDPCEQERAERAGRGLFE
                     ASGIARDDRQARRAHLRSNFQFHGAPVALILHLPADAVAGTFLEMGFFLQNIMIGLVA
                     CGLGSCPQFSVAGYADCVRSELGLGADRLIVCSLAVGYPDPQAPVNAFAPERAGLAEY
                     TRWLD"
     gene            1951..3507
                     /locus_tag="VF516_19575"
     CDS             1951..3507
                     /locus_tag="VF516_19575"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEX8109945.1"
                     /translation="MVHNLAEDDQLYKAADRASLLATRAAVVAVLSTRPAPDGAELRA
                     LAGRVDSLEVRADLVGDLDVHWLRQQFPGTLVYTLRSVEEGGQFRGDRADRHVRLQAA
                     AQGYDQVDLEWATDLTAELLAAVPAGRRRLSHHAGRLGAAAGVAELTALLDRMTATPA
                     ALYVLSIDADTAEDAVAVLSLLAAARRSDLTAYATGLPGLFTRILAPRLGAPVVFAEL
                     GTGAQAGLPTLAELQTDYGFPELVPVHALYGIVSPSLRPSAWARLHNHAYRTVGLPAL
                     FLPFATQDLRRFWHTLIPALDAARLPLRGMTVCTPFKEPVLDLVERTTAAAIHCGAGN
                     IVWREGDAWVADATDGCVVSALAAMGVELNGRRAAVVGCGAAGRNLASALILRGAEVV
                     LVNRSEDRGRFAAQLLGLPFVPLAEFSPAGFSLLVHATPVSDRAPVSLADLPSDAAVL
                     EMVPAHTTTPLIAEARARGLLTIDGWQVLAIEGDLHFLLMTGMVMPPNAALDLRAPLR
                     VVDPSLTEES"
     gene            3507..4358
                     /locus_tag="VF516_19580"
     CDS             3507..4358
                     /locus_tag="VF516_19580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109946.1"
                     /translation="MTRSYPNLALSTGKPLQISPVCGDWLNADHNGVPGMLGVTLTEQ
                     EGSLWVRGLGMAKPAGYVWPRVRASSFAPDPTSRQVWGFLVDYDFGFMRTIIAGYYKT
                     SVLVATTYNTFQDGSGRTPYWSREFFHRRMLAEPATTAAPPGVSRDRDRSQGTAPGRV
                     DPAPMIGRWVNFDREAPDIIGVTVRADGDRLAISMDQAGREGVRHWPAVPGVAMAERT
                     SGGPAIGFLAMGDLFGEQGDGPHGGALCAYLNRGLLTIDTYVTDVRTSTNVMNRIHLH
                     LAEGATV"
     gene            complement(4331..4570)
                     /locus_tag="VF516_19585"
     CDS             complement(4331..4570)
                     /locus_tag="VF516_19585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109947.1"
                     /translation="MTEPESAGLDRDRRTQEARAEQGLEARPGERRGSIVLVGPRAQC
                     LVAQPGDLAQRVANGGGGIGEQDLERGHTVAPSAR"
     gene            4744..5565
                     /locus_tag="VF516_19590"
     CDS             4744..5565
                     /locus_tag="VF516_19590"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8109948.1"
                     /translation="MVGWSGEPSAPKPGEKARFGTTVTPTADGWIINGNKAFATSASG
                     ATHAMLLVSTEGPGGARHSQGSPEGLLMLACDLSDRSISIDTSWWDPVGMRATASHLV
                     RFDNTFVSHDGLIGKPGSYLLDQWQTAFVPHYAASFLGAAEAAYEYAVKYVKHQNKVS
                     DPYIQHRIGSMGVNVDTGHLWLHHVAGLWDRGHRQEAALAGTRVRHLVEHLAEQTVHH
                     CIRACGARSLIRPSPVERILRDLTFYLRHDNDDHVLATIGREVLGERHDVSFYKP"
     gene            5617..6987
                     /locus_tag="VF516_19595"
     CDS             5617..6987
                     /locus_tag="VF516_19595"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8109949.1"
                     /translation="MTTTPPTSEPLTPPQLRYIAQFVEAYMARTPGSAAAQRAAYPFL
                     ADPRSSGGPKAPPAIREFWNATRLMRYPIVGKCSAGSRGRDIDGNEYIDFGIGLGVYL
                     FGYSPDFIVEALHRRIDTGMTMGFQSDVCRDVAEGIARMIGMERVAFANTGAEANMVA
                     LRLARAATGRNKIVVFAVSYHGSGDATIPGLGMLLGVTPGAIQDTIILEYGADASLQR
                     IAELGDQLAAVLVEPVQSRQPWLQPAAFLQELRRITAEHGTALIFDEVMQGFRIHQGG
                     CQAHFDIRADLATYGKIIGGGLPIGAIGGSARFMDRLDGGAWSDTDDSSSAVDKIWFS
                     GTFTKNPLTMASAQAVVERLSQEGPALQERLNQRAGTLVDRLSIWIREHRMPISIARF
                     GSMFRFMGSPHMTLLTHHLAMRGIYTQENMLFFVSVAHSDSDLLALEEAVKDSLVTMR
                     KGGYFA"
     gene            7037..8008
                     /locus_tag="VF516_19600"
     CDS             7037..8008
                     /locus_tag="VF516_19600"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEX8109950.1"
                     /translation="MIGPGALWMALFVAAAVLSSCKESSRDNTVSVLYAGSLATALEN
                     GLGAAFSKATGHAYKGEAQGSLGAARMIHDHLRSPDVFISADPTVNETVLMGDKNENL
                     VSWFATMASSQLVLAYNPKSSFADKFQAAAADKIPWYEVLQIPGVRFGRGDPTTDPKG
                     YRTLFLFDLAGKHYQKPEIPRMLGDPLNPAQVFPEIALLARVESGQFDAGIFYKHEII
                     ARHLPFVTLPPEINLGDPKFAGPYAEATYKTPSGDRVKGAPILFTITIPRTARHPVAA
                     EAFVRFMLSSPRLLEEFGFGTIEHQVAGDREQVPATLRGLCPGTYQP"
     gene            8005..>8229
                     /locus_tag="VF516_19605"
     CDS             8005..>8229
                     /locus_tag="VF516_19605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109951.1"
                     /translation="MMRRLTPTFLVFGALGGLLLLNLVLPIVNLFLQADGPGWMTSLR
                     EQGADDALAVSALSSAITVVITALLGVPLGY"
BASE COUNT         1296 a         2851 c         2738 g         1344 t
ORIGIN      
        1 ataggacagg cggtagtcgt acgccggtgg ccatgacaca tggttccgct cgcggtagct
       61 ggtcgcacgt tcgagattgg tgccatacgc aaagcggtcg agtttcacgc tgttcccccc
      121 ggtgttaatg cgccgtggtg cggtccctcc gcgccggttg gcggaagtca cgcaggatgg
      181 agttttacgc gagtaacaga tagcggtttt ggaagtcaac ctgaaacacg ttgagatggt
      241 gatgcaagat gatgcatggc cggcgacaca cggcattata tggcatatct ggtgtgccat
      301 cacactcgtc ccagcacggt cggtgatgcg tcgccggcgc ccgcagatcg cgcaagttcc
      361 acgcaggccc catgaattac gtcctgaagc gcccggagat caccggcctc gcgagctgcc
      421 ccggactccg ggtgtcctgg gaatttggac agccatcgtg ttaatccgga tcagaaatca
      481 cgatttttag agtcaatccg gatgagaaac cccaatgtat tccggtcaat ccggatgagg
      541 aaatcgcgat aaccggaggg gatccggcgg ggggcggatg gtgtccggga cctgacgagc
      601 tgtcctgaac tcccggacgg cgcccaggaa cccggagggc tgtcctgaac tccaggacag
      661 ctcatcggag aaacaccgag tccccacgcc agtcgcggtg cccgcgatag aatggcagcg
      721 gcagcccgat ggaccgccgc gagcgggtcg aggccgtcac ctccttgcaa atctactatg
      781 gacatctcaa tcgcgagctg atactgctcg ccgcaaagcg ctcatcgtga gaggagtacc
      841 attgcgatag cctgtccctg ccgcatcgcg acacggcatc gcggccgcgc ctggcgcagg
      901 acggcacggt cgaactggac aacagaccgt ctcgtgtccc ggcatcatca tatcgcgtct
      961 cagggaaccg gtatcgcgac gtacctaccc gcagcagatc gaagggccat gtgttctatc
     1021 tagcgctcgg atacttcatc tcgtacctgc cgtacgccat cctggccaag gggctgtcca
     1081 gcggcatggt ccccggcgtc gatgcgccgg tcggcggcct ggtgctgctg ccggccgccg
     1141 cgctcggaca gtccgtgcgc gacgtcactg gacgatcatc gccgcaccgc cgggggcatc
     1201 cagcaggccg tacggcagcg gctggcggag cagttcccgg gggcgttccc gtgatcggcg
     1261 atcgatccgc gcggccgtgc tcagtcgagc cagcgggtat attcggccag accggcgcgc
     1321 tcgggcgcga acgcgttcac cggcgcctgt gggtcggggt atcccacggc gagcgagcac
     1381 acgatcaggc ggtcggcgcc caggccgagc tccgatcgga cgcagtcggc gtagcctgcg
     1441 acgctgaact gcggacagct gccgagcccg caggccacca ggccgatcat gatgttctgc
     1501 aggaagaagc ccatctccag gaacgtcccg gccacggcgt ccgcggggag atgcaggatc
     1561 aacgccaccg gcgcgccatg gaactggaaa ttcgagcgca ggtgggcacg gcgcgcctgc
     1621 cggtcgtcgc gcgcgatgcc gctggcctcg aacaacccgc ggccggcgcg ctccgcgcgc
     1681 tcctgctcgc acgggtccgt ggtcggacgc cggttgacgt agtcgggccg ggccggctca
     1741 tcggcatcga aggcagcgca caaccgatct gccagccgca tccgcgccgc cccgctcagc
     1801 accaccacct gccagggctg ggcatttcgc gtcgagggcg catgcgcggc ggcgccgagc
     1861 acctggcgga gcagcgcctg cgggaccggc cggtccagat aggcccgctt gcaatgccgc
     1921 gcggtcatcg cggagaggac gtcgaggctc atggtgcaca acctagcaga agatgatcag
     1981 ctctataaag ctgctgatcg agcgtcgctg ttggcgacgc gcgcggcggt ggttgcggtg
     2041 ctcagcacgc gtcctgcgcc cgacggcgcc gagctgcgcg ccctggccgg tcgcgtcgat
     2101 tcgctcgagg tccgcgccga tctggtcggc gacctggatg tccactggct gcgccagcag
     2161 ttccccggaa cactcgtcta tacgctgcgc agcgtcgagg aaggtggcca gttccgcggc
     2221 gaccgcgccg atcgccatgt ccggcttcag gccgccgctc aagggtacga tcaagtcgat
     2281 ctcgaatggg ccacggatct cacggcggag ctgctcgcgg cggtgcccgc cggccggcgc
     2341 cggctgtcgc accacgccgg ccggctcggc gcggccgccg gggtcgccga gctgaccgcg
     2401 ttgctcgacc ggatgaccgc gacaccggcc gcgctgtacg tgctgtcgat cgacgccgac
     2461 acggccgagg atgcggtggc ggtgctatcg ctgctggcgg cggcccggcg atcggatctg
     2521 accgcctacg cgaccggcct gcccgggctg ttcacccgga tcctggcacc ccggctgggc
     2581 gcgcccgtcg tgttcgccga gctcggcacc ggcgcgcagg ccgggttgcc cacgctggcc
     2641 gagctgcaga cggactacgg gtttccggag ctcgtcccgg tgcacgctct gtatggcatt
     2701 gtcagtccct cgctgcgtcc atcggcctgg gcgcgcctgc acaaccacgc ctatcgcacg
     2761 gtgggtctgc ccgccctctt cctgccgttc gcgacccagg atctccggcg attctggcat
     2821 accttgatcc cggcgctgga cgcggcgcgc ttgccgctgc ggggcatgac cgtgtgcacc
     2881 ccgttcaagg agcccgtgct cgatctcgtc gagcgcacga ccgcggccgc gatccactgc
     2941 ggcgctggaa atatcgtctg gcgcgagggg gacgcgtggg tggcagatgc cacggatggc
     3001 tgcgtcgtgt ctgccctggc cgcgatgggc gtcgagctga acggtcgtcg agccgcggtg
     3061 gtgggctgtg gcgccgccgg gcgaaacctc gcctccgccc tgatcctgcg gggagccgag
     3121 gtcgtcctgg tcaaccgcag cgaggaccgt gggcggttcg ccgcgcagct gctcggattg
     3181 ccgttcgtgc cgctcgccga gttctcgccg gcgggcttct cgctgctggt gcatgccacg
     3241 ccggtgtccg atcgggctcc ggtttcgctc gccgacctgc ccagcgatgc cgcggtgctc
     3301 gagatggtgc ccgcgcacac gaccacaccg ctgatcgcgg aggcccgcgc ccgcgggctg
     3361 ctcacgatcg acggctggca agtgctcgcg atcgaagggg acctgcactt cctactcatg
     3421 accggcatgg tgatgccgcc gaacgcggcg ctcgacctgc gtgccccctt gcgggtggtc
     3481 gacccctcat tgacggagga atcctgatga cgagatcgta tccgaacctg gcgctgtcca
     3541 cgggcaaacc actccagatc tccccggtgt gcggagactg gttgaatgcg gatcacaacg
     3601 gcgtccccgg catgctcggg gtgaccttga ccgagcagga aggcagcctc tgggtgcgcg
     3661 ggctgggcat ggcgaagccg gccgggtacg tctggccccg tgtccgtgca tcgagcttcg
     3721 cgccggatcc gacatcgcgt caggtatggg gcttcctggt cgactacgac ttcggattca
     3781 tgcggaccat catcgcgggc tactacaaga ccagcgtcct ggtcgcgacg acctacaaca
     3841 ccttccagga cggcagcggg cggacgccgt actggagccg cgagttcttc caccgccgca
     3901 tgctggccga gcccgcgacg acggcggctc cccccggggt cagccgcgac cgcgaccgga
     3961 gccagggcac cgcacccggg cgcgtcgatc cggccccgat gatcggtcgc tgggtcaact
     4021 tcgaccgcga ggctccggac atcatcgggg tcaccgtgcg ggccgacggc gaccggctcg
     4081 ccatctcgat ggaccaggcc ggtcgcgagg gggttcgcca ctggccggcg gtgccggggg
     4141 tcgcgatggc cgagcgcacc agcggcgggc cggcgattgg ctttctcgcc atgggcgatc
     4201 tgttcggcga gcaaggcgat ggcccgcacg gcggcgcgct gtgcgcgtac ctgaaccggg
     4261 gactcctcac cattgacacc tatgtcaccg acgtccggac atcgaccaac gtgatgaacc
     4321 gcatccacct tcacctggcc gagggagcaa cggtatgacc acgctcgaga tcctgctcgc
     4381 cgattcctcc cccgccgttc gcgacgcgct gggcaaggtc gcccggctgg gcgacgaggc
     4441 attgcgcgcg cgggccgacg agtacgatcg aaccgcgacg ttccccaggt cgagcttcga
     4501 ggccctgttc ggcgcgggcc tcttgggtgc gacgatcccg gtcgaggccg gcggactcgg
     4561 gttcggtcat caccgcggca acacgttccc gctgtggatg atgaccaagc tgatcgccaa
     4621 ggccgacctg tcgctggcgc gctgctggga gggccacacc aacacgctcg tgctgatcga
     4681 cgcgctgggc acgccgcagc agaagcagcg ctggttcagc ggggtgctcg agcgcggtga
     4741 gatctggtcg ggtggagcgg cgaaccgagc gcgcccaagc ccggcgagaa ggcacggttc
     4801 ggtaccaccg tcacccccac ggcagacggc tggatcatca acggcaacaa ggcgttcgcc
     4861 acgagcgcca gcggcgcgac ccacgccatg ctgctggtca gcaccgaggg gccgggcggc
     4921 gcacgccata gccaggggtc gcccgagggg ctgctcatgc tggcctgcga tctctcggat
     4981 cgcagcatct ccatcgatac ctcgtggtgg gacccggtcg gcatgcgcgc gaccgcgagc
     5041 cacctggtcc ggttcgacaa cacgttcgtg tcgcacgatg gcctgatcgg caagcccggc
     5101 agctacctgc tcgatcagtg gcagaccgcg ttcgtcccgc actacgccgc gagcttcctc
     5161 ggcgccgccg aggcggccta cgagtacgcc gtgaagtacg tcaagcacca gaacaaggtg
     5221 agcgatccgt acatccagca ccggatcggc agcatgggag tcaacgtcga taccgggcat
     5281 ctctggctgc accacgtcgc gggcctgtgg gaccgcggcc accggcagga ggccgcgctc
     5341 gccggcaccc gggtgcggca cctggtcgag cacctggccg agcagaccgt gcatcactgc
     5401 atccgcgcct gcggcgctcg atccctgatc cggccgagcc cggtcgagcg catcctgcgc
     5461 gatctgacgt tttacctccg gcacgacaac gacgaccacg tcctcgcgac catcggacgc
     5521 gaggtgctgg gcgagcgcca cgacgtctcg ttctacaagc cctgagctct gcccacctgc
     5581 agccgacgcc cttcacatcc tgcgaaggcc atcctcatga ccaccacacc gcccacctcc
     5641 gagcccctca ctcccccgca actccgctac atcgcgcagt tcgtcgaggc gtacatggcc
     5701 aggacgccgg ggtcggccgc ggcgcagcgc gcggcctacc cgttcctcgc cgacccgcgc
     5761 tcgtccgggg gccccaaggc tccgccggcg atccgcgagt tctggaacgc caccaggctc
     5821 atgcgctatc cgatcgtcgg caagtgcagc gcaggatccc gtggccggga catcgacggc
     5881 aacgagtaca tcgacttcgg catcgggctc ggcgtctacc tgttcggcta cagcccggat
     5941 ttcatcgtgg aggcgttgca ccgccggatc gacaccggca tgaccatggg ctttcaatcc
     6001 gacgtgtgtc gcgacgtcgc cgaggggatc gcccgcatga tcggcatgga gcgcgtcgcg
     6061 tttgccaaca cgggcgcgga ggcgaacatg gtggcgctcc ggctcgcccg cgccgcgacc
     6121 ggccgcaaca agatcgtcgt gttcgccgtc tcctatcacg gctccggcga cgccaccatc
     6181 ccggggctcg gcatgttgct cggcgtgacg cccggtgcca tccaggacac gatcatcctg
     6241 gagtacggcg ccgacgcgtc gctacagcgc atcgccgagc tcggcgatca gctcgccgca
     6301 gtgctcgtcg agcccgtgca gtcgcgccag ccctggctcc agccggcggc cttcctccag
     6361 gagctccggc gcatcaccgc cgagcacggc accgcgctga tcttcgatga ggtcatgcag
     6421 gggttccgga tccaccaggg cggttgccag gcgcacttcg acatcagggc cgacctcgcg
     6481 acctacggca agatcatcgg cggcggcctg ccgatcggcg cgatcggcgg gagcgctcgg
     6541 ttcatggacc ggctcgatgg cggcgcgtgg agcgacaccg acgactcctc gtccgcggtc
     6601 gacaagatct ggttctccgg caccttcacc aagaatccgt tgaccatggc cagcgctcag
     6661 gccgtcgtgg agcgcctctc gcaagagggc cccgcgctgc aagagcgcct caaccagcgc
     6721 gccgggaccc tggtggaccg gctctcgatc tggatccgcg agcaccgcat gccgatctcg
     6781 atcgcccgct tcggctcgat gttccgcttc atgggctcgc cgcacatgac cctgctcacc
     6841 caccacctcg cgatgcgagg catctatacc caggagaaca tgctgttctt cgtctccgtc
     6901 gcacattccg acagcgacct gctcgcgctc gaggaagcgg tgaaggacag cctggtcacg
     6961 atgcgcaagg gcggctactt cgcgtgagtc ccggcatgtc gctcaagaca ggtggtttat
     7021 gacgcgagct cgttcgatga tcggtcctgg agcgctctgg atggcgctgt tcgttgcggc
     7081 ggcggtcctg tcctcgtgca aggagagctc tcgggacaac acggtctcgg tcttgtatgc
     7141 gggctccctg gccaccgccc tggagaacgg gctcggcgct gcgttctcga aagcgaccgg
     7201 acacgcctac aagggcgaag cgcaagggtc gctcggggcc gcgcgcatga tccacgatca
     7261 tctgaggagc ccggacgtct tcatcagcgc ggatccgacg gtcaacgaga ccgtgctgat
     7321 gggcgacaag aacgagaacc tcgtgtcgtg gttcgcgacg atggcgtctt ctcagctcgt
     7381 gctcgcctac aatccgaaga gctcgttcgc ggacaagttt caggccgcgg cggccgacaa
     7441 gattccctgg tacgaggtcc tccagatccc cggcgtgcgc ttcgggaggg gcgatccgac
     7501 gaccgatccg aagggatacc ggacgctctt cctcttcgat ctggcgggga aacactatca
     7561 gaagcccgag atccctcgca tgctgggtga tccgctgaac cccgcccagg tgttccccga
     7621 gatcgcgctg ctggcccgcg tcgagagcgg ccagttcgac gccgggatct tttacaagca
     7681 cgagatcatc gctcgccact tgccgttcgt cacgttgcca cccgagatca acctgggcga
     7741 cccgaagttc gccggcccct acgcggaggc cacctacaag acgccgtccg gcgatcgagt
     7801 caagggcgct ccgatcctgt tcacgatcac gattcccagg accgcgcgtc atccggttgc
     7861 tgccgaggcc ttcgtgcggt tcatgctgtc gtcgcccagg ctcctggagg agttcggttt
     7921 cgggaccatc gagcaccagg tggccggcga tcgggagcag gttccggcaa ccctccgcgg
     7981 tctttgcccg gggacctacc agccatgatg cggcgcctca ctccgacgtt cctcgtgttc
     8041 ggcgcgctgg gcggcctgct gctgctgaac ctcgtgctgc cgatcgtcaa cctgttcctc
     8101 caggccgacg gcccgggctg gatgacgtcg ttgcgagagc aaggcgcaga cgatgcgctg
     8161 gcggtctctg cgctgtcctc cgcgatcacc gtggtgatca ccgcgctgct cggcgtcccg
     8221 ctcggatac
//
LOCUS       DASVOR010001012         2230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156407,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001012 DASVOR010000000
VERSION     DASVOR010001012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156407"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..>2230)
                     /locus_tag="VF516_19610"
     CDS             complement(159..>2230)
                     /locus_tag="VF516_19610"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX8109952.1"
                     /translation="ALAEVERLTALANQDPYLAPWTGPHGGVPPFDKLQADAFPRAFE
                     LGLALQLAEVEAIAQNPEPPSFENTFVPLEDSGRHVGRAETLFSVMTSSLSTPEVHAV
                     EKQWSPKIAAARDKITFNDKLFARVAALYAARDQLDPERRRLVELRYDRFVKAGAKLS
                     AEDKARVGAINEQLAGLYTEFNKKVLADENSWIELGPGDLAGLTAQLKATYAAAAAER
                     KLDGKWAVVNTRSSVDPFLAASARRDLREKVWKAFKQRGDNGGDNDTHATFARIVKLR
                     ADRARLLGYASHAHWRMSDTMARDPAKAMDLMMQVWKPAVARVREEVADMEKLARRDG
                     MKGAFEPWDYLYYAEKVRKAKYDLDENELKPYFELNNMIAASQWMAEKLYGLTFTEIT
                     GTVPVFEPSVRVWQVKDKTGAHVGLFYGDYFARKGKRSGAWTAGYRRHETFTGKVETP
                     IASNNNNFVRGAPGEPVLISLDDARTLFHEFGHALHALLSEVNYPGLGRTPQDFVEYP
                     SQVHEFWVLSRPILDRFAKHYKTGKLMPQALVDKVTRASKFNQGYATVEYLSSAIVDM
                     KLHTIPDGAIDAERFERDALDEIRAPREVAMRHRLPQFSHLFASDAYSAGYYSYLWSE
                     VMDADTREAFTEAGDVFDPTVAGKLRTYILAPGDSTDRAEAYRQFRGRDPDVAALLKK
                     RGFPIAK"
BASE COUNT          303 a          765 c          781 g          381 t
ORIGIN      
        1 cctgcagcat caccgggccg ggcggcacgt ccgcgatgct gtccggaacc tcgaacctgg
       61 tcccccggga cggcaacacg gtcgctatct gggcgttcgg agcgaccgtg cagttcggct
      121 cggtcgagat cgtcggcaag ccctagtggc tatgaccgct acttcgcgat cgggaacccg
      181 cgcttcttga gcagcgccgc gacgtcggga tcgcggccgc ggaactggcg atacgcctcg
      241 gcgcgatcgg tcgagtcgcc gggcgcgagg atgtacgtgc gcagcttgcc ggcgacggtc
      301 ggatcgaaga cgtcgccggc ctcggtgaac gcctcgcggg tgtcggcgtc catcacctcg
      361 gaccacaggt agctgtagta gcccgcggag taggcgtcgc tggcgaacag gtggctgaac
      421 tgcggcaggc ggtggcgcat cgcgacctcg cggggcgcgc ggatctcgtc cagcgcgtcg
      481 cgctcgaacc gctcggcatc gatcgcgccg tcggggatcg tgtgcagctt catgtcgacg
      541 atcgccgacg acaggtactc gaccgtggcg tagccctggt tgaacttgct cgcccgggtc
      601 accttgtcga ccagcgcctg tggcatcagc ttgccggtct tgtagtgctt ggcgaagcgg
      661 tcgaggatcg gccggctcag cacccagaac tcgtggacct gcgacgggta ctcgacgaag
      721 tcctgcggcg tgcggcccag gcccgggtag ttgacctcgg acagcagggc gtgcagcgcg
      781 tggccgaact cgtggaacag ggtgcgcgca tcgtcgagcg agatcagcac cggctcgccg
      841 ggcgcgccgc ggacgaagtt gttgttgttc gacgcgatcg gggtctcgac cttgccggtg
      901 aaggtctcgt gccggcggta gcccgcggtc cacgcgcccg agcgcttgcc cttgcgcgcg
      961 aagtagtcgc cgtagaacag cccgacgtgc gcgccggtct tgtccttgac ctgccagacc
     1021 cgcacgctgg gctcgaacac cgggacggtc ccggtgatct cggtgaacgt caggccgtac
     1081 agcttctcgg ccatccactg cgacgccgcg atcatgttgt tgagctcgaa gtacggcttg
     1141 agctcgttct cgtcgaggtc gtacttcgcc ttgcgcacct tctcggcgta gtacaggtag
     1201 tcccagggct cgaacgcgcc cttcatgccg tcgcggcggg cgagcttctc catgtcggcg
     1261 acctcctcgc ggacgcgcgc caccgccggc ttccacacct gcatcatcag gtccatcgcc
     1321 ttggccggat cgcgcgccat ggtgtcggac atccgccagt gcgcatggct ggcgtagccg
     1381 agcagccgcg cgcgatccgc ccgcagcttg acgatccgcg cgaacgtcgc gtgggtatcg
     1441 ttgtcgccgc cgttgtcgcc gcgctgcttg aacgccttcc acaccttctc gcgcaggtcg
     1501 cgccgcgccg acgccgcgag gaacggatcg acgctcgacc gggtgttgac caccgcccac
     1561 ttgccgtcga gcttgcgctc cgcggcggcc gcggcgtagg tcgccttgag ctgcgcggtc
     1621 agcccggcga ggtcgcccgg accgagctcg atccacgagt tctcgtcggc gagcaccttc
     1681 ttgttgaact cggtgtacag gccggcgagc tgctcgttga tcgcgccgac ccgggccttg
     1741 tcctcggccg acagcttcgc gccggccttg acgaaccggt cgtagcgcag ctcgaccagc
     1801 cggcggcgct cggggtcgag ctgatcgcgc gcggcgtaca gcgcggcgac ccgcgcgaac
     1861 agcttgtcgt tgaacgtgat cttgtcgcgc gcggccgcga tcttcggcga ccactgcttc
     1921 tcgaccgcgt ggacctcggg cgtgctgagg ctactggtca tcaccgagaa cagggtctcg
     1981 gcgcgaccga cgtggcggcc gctgtcctcg agcggcacga acgtgttctc gaagctcggc
     2041 ggctcggggt tctgcgcgat cgcctcgacc tcggcgagct gcagcgcgag cccgagctcg
     2101 aacgcgcgcg ggaacgcgtc ggcctgcagc ttgtcgaacg gcgggacgcc gccgtgcggc
     2161 ccggtccacg gcgcgaggta cggatcctgg ttggcgagcg cggtcagccg ctcgacctcg
     2221 gcgagcgcgg
//
LOCUS       DASVOR010001013         6206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_244057,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001013 DASVOR010000000
VERSION     DASVOR010001013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6206
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244057"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1933)
                     /gene="mrdA"
                     /locus_tag="VF516_19615"
     CDS             complement(<1..1933)
                     /gene="mrdA"
                     /locus_tag="VF516_19615"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEX8109953.1"
                     /translation="MLIRQSSGPGSGNGPALSELHRRLRWVALIGIGALVVLVGRLWQ
                     LQVMRGEGYYERTVSNVVKERYLPSVRGKILDRNGVSLADNRPAFNIYATPRQLTREL
                     EAELVRMLGLSDSEIAKIDERLAVGRKRDPRTAIVILEDQGRDRAALIEQSLARLPGV
                     EVHHEPYRYYPQGDLAAHLIGYMTQMTAAEADRLTNQGYDASELVGRYGLEEKWENYL
                     RGKKGIERYAVDARGQRLDEATASALIQGEHGVPAIPGANVVLTVDAQLQKLAEKAVS
                     RVAAAAVVVVEPRTGKLRAVVSKPSFDPNIMTGHLTQAEETMLKQDPRKPFVDKALRA
                     TYPPGSIFKFTTALAALEARQASVDEAMFCPGEYQLSGTVFRCHGTHGKVDLLQAIQH
                     SCDFYFWKLAERIGIDPIAQVATEYGLGKPTNLGLNGDSAGRIPTKDWYDRTSHYRAG
                     FAANTAIGQGDVEVTVLQMAMAYAALANGGTLYVPQVVERLEAHDGRVITSYEPQIAH
                     LIKTPGDAVAVWKRGMWKVTNELGGTAFEHGHTDLVPVMGKTGTAEVKKHHKDGDDKD
                     LERWNPSADHAWFAGWAPADDPEIVIVVMVEHGGGGGAVAAPIAKQILEGYFTKIHPL
                     PRPAAPAGSAGPAGRPAPAA"
     gene            complement(1956..2492)
                     /locus_tag="VF516_19620"
     CDS             complement(1956..2492)
                     /locus_tag="VF516_19620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109954.1"
                     /translation="MRTATIVLVAYLMCVAVAALWRFMPWIADAIPDLGALTAAYLGL
                     TARRQVSPAIGGAIVLGYLIDLISGAPPGLVALVLGLTTLIARAVQQRILVRGAVISV
                     AFSAFVALIAGVLGYLVRELYRVPVAPFAIELRHVAGVTVATAIVGPLVWRVFRRIDA
                     AFARTHREREAALEGIAP"
     gene            complement(2489..3418)
                     /gene="mreC"
                     /locus_tag="VF516_19625"
     CDS             complement(2489..3418)
                     /gene="mreC"
                     /locus_tag="VF516_19625"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEX8109955.1"
                     /translation="MTLRRRLVDWSLTAVLIVLPALVLRASLARGTPSALDQALLRLT
                     APLETGISWVVEGIAGTWSRYVALVDVEAENRELREDNERLRKQLAAMARRAYDVAAL
                     EDIAAVKKRTPADTLGARVIGAPLSPQFRVVRLRIDRGGREVATDMPVITSEGPVGRI
                     DKVYGDYADVVLISDPASHIDVVIRDPAQPAGQASGARGILKGLGRPDSYACTIEWLE
                     RQGGPATQSRVKVGDEVVTSGLGASFPAGLIVGKITRVGGDDGMFQSVEVRPTVDVSR
                     VRAVMVLLAPPPPPDPDAKTRRRSEAAFGARPL"
     gene            complement(3427..4461)
                     /locus_tag="VF516_19630"
     CDS             complement(3427..4461)
                     /locus_tag="VF516_19630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEX8109956.1"
                     /translation="MLFDWVYGMFSNDLAIDLGTATTLTFVKGRGIVANEPSVVAVQA
                     SSQGPKKVLAVGKEAKEMLGRTPGNIVAIRPMKDGVIADFEVTEAMLRYFIQKAHQRR
                     TLVRPRVIVSVPSGITEVEKRAVRDSALAAAAREVYLIEEPMAAAIGGGLPITEPTGN
                     MIVDIGGGTTEVAVIALAGVVLSKSIRVAGDKMDEAIVQYIKRKYNLLIGERTAEIIK
                     KEIGSAYPLEEPLTVEVKGRDLVAGVPKTLMVNSDEIREALQEPVNAIVDAVRSVLER
                     TPPELSADIVDKGIVLSGGGAQLKNLDVLLREETGLPVMVCENPQLAVVLGTGKVLDE
                     LGLLREIALR"
     gene            4716..>6206
                     /locus_tag="VF516_19635"
     CDS             4716..>6206
                     /locus_tag="VF516_19635"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEX8109957.1"
                     /translation="MLENTRRQASIFVYIIFGLLIVIFIYGINPGNRGGREGGGCSVN
                     NNSVVTVNGSDAGESAYRIAYSMSQGQSRQRTYVTLDRIVFRELLAQAAEDRGIRTNG
                     DLIDDEIKSQHGYFYLGGERIDVSSQFFEDGFYKHRRWLDWVAARNMQSPGAYKEEQA
                     RSLQAALMADLITHSVRVSRDEALQSYLYDNNTVTYDVVAFDPGRYRRAMRLTDADTK
                     RYLDAHTAEVEARYKADERTYKGVKPQLQLREIFIPKAAPEAAKPDDDARPDDKAAKP
                     DDKAKPDDKAKPGDKTAKAAKPDDKTAKADAADKKPAAGAAAKPHGLPTDVAKTKLEA
                     LRAEIAAGKLAFPDAEKRVAADASDDAPAENGDRGWQSVDKLGLGDKAVNDAVKALKP
                     GEMTPVIVTDRGVYLVMATAKREGDLGFDQVKMEIAAGLAKDAWSKEGAKRAALDALA
                     KVQASGKGLDQMFEKEQAAPQGGGIEQLLQDPNLTQEQKQKILEQLI"
BASE COUNT          953 a         2167 c         2211 g          875 t
ORIGIN      
        1 cggcagccgg agccgggcgc ccggcaggcc cggcggaacc ggccggggcg gcggggcgcg
       61 gcagcgggtg gatcttggtg aagtagccct cgaggatctg cttggcgatc ggcgccgcga
      121 ccgcgccgcc gccgccgccg tgctcgacca tcacgacgat gacgatctcg gggtcgtcgg
      181 ccggggccca gccggcgaac caggcgtggt cggcgctggg gttccagcgc tcgaggtcct
      241 tgtcgtcgcc gtccttgtgg tgcttcttga cctcggcggt gccggtcttg cccatcacgg
      301 ggacgagatc ggtgtggccg tgctcgaacg cggtgccgcc gagctcgttg gtgaccttcc
      361 acatgccgcg cttccacacc gcgaccgcgt cgccgggggt cttgatcagg tgcgcgatct
      421 gcggctcgta gctggtgatc acgcggccgt cgtgggcctc gagccgctcg acgacctggg
      481 gcacgtacag cgtcccgccg ttggcgagcg cggcgtaggc catcgccatc tgcagcacgg
      541 tgacctcgac gtcgccctgc ccgatcgccg tgttggccgc gaacccggcg cggtagtggc
      601 tggtacggtc gtaccagtcc ttggtcggga tccggccggc agagtcaccg ttgagcccga
      661 ggttggtcgg cttgcccagg ccgtactcgg tcgcgacctg cgcgatcggg tcgatgccga
      721 tccgctcggc gagcttccag aagtagaagt cgcacgagtg ctggatcgcc tggagcagat
      781 cgaccttgcc atgcgtgccg tggcagcgga acaccgtgcc cgacagctgg tactcaccgg
      841 ggcagaacat ggcctcgtcg acggaggcct ggcgggcctc gagcgcggcg agcgcggtgg
      901 tgaacttgaa gatcgagccc ggcgggtagg tcgcgcgcag cgccttgtcg acgaacggct
      961 tgcgcggatc ctgcttgagc atggtctcct cggcctgggt caggtggccg gtcatgatgt
     1021 tggggtcaaa cgacggcttg ctgaccaccg cgcggagctt gccggtccgg ggctcgacga
     1081 cgacgaccgc cgcggcggcg acgcgggaca ccgccttctc ggcgagcttc tggagctggg
     1141 cgtcgaccgt caggacgacg ttggcgcccg ggatcgccgg cacgccgtgc tcgccctgga
     1201 tcagcgcgga cgcggtggcc tcgtcgagcc gctggccgcg cgcgtcgacc gcgtagcgct
     1261 cgatgccctt cttgccgcgc aggtagttct cccacttctc ctcgaggccg tagcggccga
     1321 ccagctcgct ggcgtcgtag ccctggttgg tgagccggtc ggcctcggcc gcggtcatct
     1381 gggtcatgta gccgatcagg tgggcggcga gatcgccctg cgggtagtag cggtacggct
     1441 cgtggtggac ctcgacgccg ggcagccgcg ccagcgactg ctcgatcagc gcggcgcgat
     1501 cgcggccctg atcctcgagg atgacgatcg cggtccgcgg gtcgcgcttg cgaccgaccg
     1561 cgagccgctc gtcgatcttg gcgatctcgc tgtcggacag gccgagcatg cggaccagct
     1621 cggcctcgag ctcgcgggtc agctgcctgg gggtcgcgta gatgttgaac gccggccggt
     1681 tgtcggcgag cgacacgccg ttgcggtcga ggatcttgcc gcgcaccgac ggcaggtagc
     1741 gctccttgac cacgttggac acggtgcgct cgtagtagcc ctcgccgcgc atgacctgga
     1801 gctgccacag ccggccgacc aggacgacga gcgcgccgat gccgatcagc gcgacccagc
     1861 gcagccggcg gtgcagctcg ctgagcgccg ggccgttgcc cgagcccggc cccgacgact
     1921 ggcggatcaa cacgccggcc tcgcgatcgc ggcggtcatg gcgcgatccc ctcgagcgcg
     1981 gcctcgcgct cgcggtgggt gcgggcgaac gcggcgtcga tccggcggaa cacccgccac
     2041 accagcgggc cgacgatcgc ggtcgccacg gtcaccccgg cgacgtggcg gagctcgatc
     2101 gcgaacggcg cgaccgggac gcggtacagc tcgcggacca ggtagccgag cacgccggcg
     2161 atcagcgcga cgaacgccga gaacgcgacc gagatcaccg cgccgcgcac caggatgcgc
     2221 tgctggaccg cgcgcgcgat cagcgtggtc aggccgagca ccagcgcgac caggcccggc
     2281 ggcgcgcccg agatcaggtc gatcaggtag ccgagcacga tcgcgccgcc gatcgccggc
     2341 gacacctggc gccgcgcggt cagcccgagg taggcggcgg tcagcgcgcc gagatcgggg
     2401 atcgcgtcgg cgatccatgg catgaaccgc cacagcgccg cgaccgcgac gcacatcagg
     2461 tacgcgacca ggacgatggt tgcggtcctc acagcggccg ggctccgaac gcggcctcgc
     2521 tgcgccgccg ggtcttggcg tcgggatcgg gcggcggcgg cggcgcgagc agcaccatca
     2581 cggcgcgcac ccgcgacacg tcgaccgtgg gcctcacctc gacgctctgg aacatcccgt
     2641 cgtcgccgcc gacccgggtg atcttgccga cgatcagccc ggccgggaag ctggcgccca
     2701 ggccgctggt cacgacctcg tcgccgacct tgacccggga ctgggtcgcc ggtccgccct
     2761 ggcgctcgag ccactcgatc gtgcacgcgt aggagtccgg ccggcccagg cccttgagga
     2821 tgccgcgcgc gccgctggcc tggcccgccg gctgcgcggg atcgcggatc acgacatcga
     2881 tgtgcgacgc ggggtcgctg atcagcacga cgtcggcgta gtcgccgtag accttgtcga
     2941 tccggccgac cgggccctcc gaggtgatca ccggcatgtc ggtcgcgacc tcgcggccgc
     3001 cgcgatcgat gcggagccgg accacgcgga actgcggcga cagcggcgcc ccgatcacgc
     3061 gggcgccgag cgtgtccgcc ggcgtgcgct tcttgaccgc ggcgatgtcc tcgagcgccg
     3121 cgacgtcgta ggcccggcgc gccatcgcgg cgagctgctt gcgcagccgc tcgttgtcct
     3181 cgcgcagctc gcggttctcg gcctcgacgt cgaccagcgc gacgtagcgc gaccaggtgc
     3241 ccgcgatgcc ctcgacgacc cacgagatcc cggtctcgag cggcgcggtg agccgcagca
     3301 gcgcctgatc gagcgcggac ggcgtgccgc gcgccaggct ggccctcagc acgagggccg
     3361 gcaggacgat cagaacggcg gtgagcgacc agtcgacgag ccggcggcgc agcgtcatgg
     3421 acgcgcttac cgtagcgcga tctcgcggag caggccgagc tcgtcgagga ccttgccggt
     3481 gccgagcacg accgcgagct ggggattctc gcagaccatc accggcaggc cggtctcctc
     3541 gcggagcagc acgtcgaggt tcttgagctg tgcaccgccg cccgagagca cgatgccctt
     3601 gtcgacgatg tcggccgaca gctcgggcgg cgtgcgctcg agcaccgacc gcacggcgtc
     3661 gacgatcgcg ttgaccggct cctggagcgc ctcgcggatc tcgtcgctgt tgaccatcag
     3721 ggtcttgggc acgccggcga ccagatcgcg gcccttgacc tcgacggtca gcggctcctc
     3781 gagcgggtac gccgagccga tctccttctt gatgatctcg gcggtgcgct cgccgatcaa
     3841 gaggttgtac ttgcgcttga tgtactggac gatcgcctcg tccatcttgt cgccggcgac
     3901 gcggatcgac ttggacagca ccacgccggc gagcgcgatc accgcgacct cggtggtgcc
     3961 gccgccgatg tcgacgatca tgttgccggt cggctcggtg atcggcaggc cgccgccgat
     4021 cgccgccgcc atcggctcct cgatcaggta gacctcgcgc gccgccgcgg cgagcgccga
     4081 gtcgcgcacc gcgcgcttct cgacctcggt gatccccgac gggacgctga cgatcacgcg
     4141 cgggcggacc agggtgcggc gctggtgcgc cttctggatg aagtagcgca gcatcgcctc
     4201 ggtgacctcg aagtcggcga tcacgccgtc cttcatcggg cggatcgcga cgatgttgcc
     4261 cggcgtgcgg ccgagcatct ccttggcttc cttgccgacg gcgaggacct tcttggggcc
     4321 ctgagaagat gcctgaacgg cgacgacgct gggctcattg gcgacgatcc cgcggccttt
     4381 cacgaaggtc agcgtcgtcg cggtcccgag atcgatcgcg aggtcgttgg aaaacattcc
     4441 gtagacccag tcgaagagca tggtgattcc tggcgtagac gcgctgcggg cgcggcggag
     4501 gggagacggc tgggaaaaag acagggctgg tgcacctcgg taccacccac cgagtagcca
     4561 agcggagtta tcacgcgcct gtccgcaggc gcaagacaat cttacctcta cgaggtttgc
     4621 tcatcgcgac ggcgttccgg ggcggcctgc ggcttgtcgc cggccccccc gatggctagg
     4681 atgccccgct tgccgtagaa ccggccgagt cgctcatgct ggaaaacacg cgccgccagg
     4741 cctcgatctt cgtctacatc atcttcggtc tgctgatcgt catcttcatc tacgggatca
     4801 acccggggaa tcgaggcggc cgcgaagggg gtggctgcag cgtcaacaac aactccgtgg
     4861 tcaccgtcaa cggctccgac gcgggagaaa gcgcgtaccg gatcgcgtac tcgatgtccc
     4921 aggggcagtc gcggcagcgc acgtacgtca cgctcgaccg gatcgtcttc cgtgagctcc
     4981 tggcccaggc cgccgaggac cgcgggatcc gtacgaacgg ggatctgatc gacgacgaga
     5041 tcaagagtca gcacggctac ttctacctgg gcggcgagcg gatcgacgtc tcgtcgcagt
     5101 tcttcgagga cgggttctac aagcaccggc gctggctcga ctgggtcgcg gcgcggaaca
     5161 tgcagtcgcc cggcgcctac aaagaggagc aggcccggag cctgcaggcc gccctgatgg
     5221 cggacctgat cacccactcg gtccgggtct cgcgcgacga ggcgctgcag agctacctgt
     5281 acgacaacaa cacggtgacc tacgacgtgg tcgcgttcga cccgggccgg taccgccgcg
     5341 cgatgcgcct gaccgacgcc gacaccaagc gctacctcga tgcccacacc gccgaggtcg
     5401 aggcgcgcta caaggccgac gagcgcacct acaagggcgt caagccgcag cttcagctcc
     5461 gcgagatctt tatccccaag gcggcgccgg aggccgccaa gcccgacgac gacgccaggc
     5521 cggacgacaa ggccgccaag ccggacgaca aggccaagcc ggacgacaag gccaagcccg
     5581 gcgacaagac cgccaaggcc gccaagccgg acgacaagac cgccaaggcg gatgccgcgg
     5641 acaagaagcc cgccgccggc gccgccgcca agccgcacgg gctgccgacc gacgtcgcca
     5701 agaccaagct cgaggcgctc cgcgccgaga tcgccgccgg caagctggcg ttccccgatg
     5761 ccgagaagcg ggtcgcggcg gacgcctccg acgatgcgcc ggccgagaac ggcgaccgcg
     5821 gctggcagtc ggtcgacaag ctcgggctcg gcgacaaggc ggtcaacgac gcggtcaagg
     5881 cgctcaagcc cggcgagatg acgcccgtga tcgtcaccga tcgcggcgtg tacctggtga
     5941 tggccacggc caagcgcgag ggcgacctcg gcttcgacca ggtcaagatg gagatcgccg
     6001 cgggcctggc caaggacgcc tggagcaagg agggcgccaa gcgcgccgcg ctcgacgcgc
     6061 tcgccaaggt gcaggccagc ggcaagggcc tcgaccagat gttcgagaag gagcaggccg
     6121 ctccccaggg cggcggcatc gagcagctgc tccaggaccc caacctgacc caggagcaga
     6181 agcagaagat cctggagcag ctgatc
//
LOCUS       DASVOR010001014         1852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156408,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001014 DASVOR010000000
VERSION     DASVOR010001014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156408"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1717)
                     /locus_tag="VF516_19640"
     CDS             complement(<1..1717)
                     /locus_tag="VF516_19640"
                     /inference="COORDINATES: protein motif:HMM:NF025141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX8109958.1"
                     /translation="MKVAIRPWPCVAVVCMLSGATHATRPRPPDPADAVSEGRLVTQR
                     GGARIDVPLQHTDVKITVDGFLAEATVTQRFRNPYPSKIEAVYLFPLPTGAAVSALQI
                     TSGDRVIEGTIQERRQATRTYEAARSQGQIAALLTQERPNLFTQSVANLEPDAVVDVT
                     LHYVQRLGYDSGGYEVVFPMVAGPRYVPAAAAGKADPAAVQPAVLPAGVRSSHDIGLA
                     VELDAGVPLGDIASSSHRIAIERVPGAPSRARVRIAPDDTIPNKDFVLRYQVAGAAPA
                     FGALAYRGDPGKDGSFVLVAQPPATAAPAQITPREIVFVLDTSSSMRGAPLAKAKDVI
                     RAVLGRLRPDDTFQIVRFDDRASALGASPIANRPRNLALTLDWLAALDAGGTTEMTAG
                     IAAALAVPHDPRRLRIVAFLTDGYVGNEDDVIRLIADRAGEARLFAFGVGTAVNRYLL
                     EEVAAAGRGTAQFVRPDEPTAPVVAAFEQRIDAPVLTDVAIDWGGLTVSDVTPRALPD
                     LFVGEPLAVSGHYAHGGRATVTIRGKQAGRDVRFVVPVVLPERAVDRRETGGGDPDGS
                     AGGAGG"
BASE COUNT          235 a          689 c          690 g          238 t
ORIGIN      
        1 cgccccccgc cccgccggca gagccgtcgg ggtccccacc ccccgtctcc cttcggtcga
       61 ccgcgcgctc gggcagcacg accgggacca cgaagcggac gtcgcggccc gcctgcttgc
      121 cgcggatcgt cacggtggcc cggccgccgt gcgcgtagtg gccggacacc gcgagcggct
      181 caccgacgaa cagatcgggc agcgcgcggg gggtcacgtc cgacaccgtg aggccgcccc
      241 agtcgatcgc gacgtcggtc agcaccgggg cgtcgatccg ctgctcgaac gcggcgacca
      301 ccggcgcggt cggctcgtcg ggccggacga actgcgcggt gccgcgcccg gccgccgcga
      361 cctcctccag caggtagcgg ttgaccgcgg tcccgacgcc gaacgcgaac agccgcgcct
      421 cgccggcgcg gtcggcgatc agccggatca cgtcgtcctc gttgccgacg tagccgtcgg
      481 tgaggaacgc gacgatccgc agccgccggg gatcgtgcgg aaccgcgagc gccgccgcga
      541 tccccgcggt catctcggtc gtgccgccgg cgtcgagcgc ggccagccag tcgagcgtca
      601 gcgcgaggtt gcgcggcctg tttgcgatcg gcgatgcgcc gagcgcgctc gcgcgatcgt
      661 cgaaccggac gatctggaac gtgtcgtcgg gccgcagccg gccgagcacc gcgcggatca
      721 cgtccttggc cttggcgagc ggcgcgccgc gcatcgacga cgaggtgtcg agcacgaaca
      781 cgatctcgcg cggggtgatc tgcgccggcg ccgcggtggc gggcggctgc gcgaccagca
      841 cgaagctgcc gtccttgccc ggatcgccgc ggtaggcgag cgcgccgaac gccggcgcgg
      901 cgccggcgac ctggtagcgc aggacgaagt ccttgttcgg gatcgtgtcg tcgggggcga
      961 tccgcacgcg ggcgcggctc ggtgcacccg ggacgcgctc gatcgcgatc cggtgcgagc
     1021 tcgacgcgat gtcaccgagc ggcacgccgg cgtcgagctc gaccgcgagg ccgatgtcgt
     1081 gcgagctgcg cacgccggcc ggcagcaccg cgggctgcac cgcggccgga tccgccttgc
     1141 cggccgccgc cgcgggcacg tagcgcggtc cggcgaccat cgggaacacg acctcgtagc
     1201 cgccgctgtc gtagccgagc cgctggacgt agtgcagggt gacgtcgacc accgcgtccg
     1261 gctccaggtt ggcgaccgac tgggtgaaca ggttgggccg ctcctgcgtg agcagcgcgg
     1321 cgatctggcc ctgcgagcgg gcggcctcgt aggtccgcgt cgcctggcgg cgctcctgga
     1381 tcgtcccctc gatcacccgg tcgccgctgg tgatctgcag cgcgctgacc gcggcgccgg
     1441 tcggcagcgg gaacaggtag accgcctcga tcttcgacgg atacgggttc cggaaccgct
     1501 gggtcaccgt ggcctcggcg aggaagccgt cgacggtgat cttcacgtcg gtgtgctgga
     1561 gcggcacgtc gatccgggcg ccgccgcgct gggtgacgag ccggccctcg ctgaccgcgt
     1621 cggccggatc gggcggcctg ggccgcgtcg catgggtagc gccggacagc atgcagacca
     1681 cggccacgca gggccacggc cggatcgcga ccttcatggg ctccctttcc gcacccgccg
     1741 gcaaggcggg caatcactgc ctcggacacc gcgcctgccg gccggtttcc acgaatctct
     1801 cgcctcgcgc aaaagtgccg gccggcacgg gcgccatctg taccggtcgc gc
//
LOCUS       DASVOR010001015         2317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_302609,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001015 DASVOR010000000
VERSION     DASVOR010001015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302609"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..226)
                     /locus_tag="VF516_19645"
     CDS             complement(<1..226)
                     /locus_tag="VF516_19645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX8109959.1"
                     /translation="MISRFAPSTTGEAHPGTLLAALLVWLDARSRGGRVLLRLEDLDV
                     TRTRAAWADQMIEACRWLGLDWDAVVVQSDR"
     gene            complement(223..873)
                     /locus_tag="VF516_19650"
     CDS             complement(223..873)
                     /locus_tag="VF516_19650"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX8109960.1"
                     /translation="MLPIILKLKLATAAAAGLAAAAGPGAVPVDALVMTVAERAAAPI
                     EVKLYDENLRVSATVGIRRDGTMDDATAAEVKHLFRCRTTGRQHAIARRTLAMLADLA
                     EKYEPRTIEFVSVYRVQAGESPTSPHRDARAIDFRIRGVPLTQIRDYLWRNYTEVGIG
                     WYPAEQFIHMDSRPTLHDTAWTFLAGENHYHPYWAEVARRPAAEVAVTPPRHRSGV"
     gene            1094..2254
                     /locus_tag="VF516_19655"
     CDS             1094..2254
                     /locus_tag="VF516_19655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8109961.1"
                     /translation="MKFTEEHLQLRRTVREFVDKEINPHYDAWEAAGAFPAHEVFKKA
                     GRLGLLGVNKPEAYGGLGLDYSYQMCVIEELGTCRGGSVTMALGVQMDMATPALARWG
                     SDELRREFLVPAIAGDVVTSIAVSEVSGGSDVAAIKTTARKDGADYVINGSKMWITSA
                     AQADWLCLLVNTGDGSPHRNKTLVIVPTKLPGVTIGPKIDKLGMRASDTCPIYFEDVR
                     VPQRHRIGEEGMGFMMQMVQFQEERLNGAISSVRGMDRTLDATIAYCKERHTFGQPLI
                     DNQVIHFRFAELKTEIEALRSLVYRAVEDYVGGADVTMMASMAKLKAGRLAREVSDAC
                     LQYWGGMGFTWDNPAARSYRDSRLLSIGGGADEIMLGIICKLMGILPGKKKK"
BASE COUNT          364 a          802 c          813 g          338 t
ORIGIN      
        1 gccggtcgct ctgcacgacc acggcgtccc agtccaggcc gagccagcgg cacgcctcga
       61 tcatctggtc ggcccacgcc gcccgggtcc gggtgacgtc gaggtcctcg agccgcagca
      121 ggacccggcc gccgcgcgac cgggcgtcga gccagaccag cagcgcggcg agcagcgtcc
      181 ccgggtgcgc ctcgccggtg gtcgacggcg cgaaccgcga gatcacaccc cggagcggtg
      241 gcgcggtggc gtcaccgcga cctcggccgc cggccggcgc gcgacctcgg cccagtacgg
      301 gtggtagtga ttctcgccgg cgaggaacgt ccacgcggtg tcgtgcagcg tcggccgcga
      361 gtccatgtgg atgaactgct ccgcggggta ccagccgatg ccgacctcgg tgtagttccg
      421 ccacaggtag tcgcggatct gggtcagcgg cacgccgcgg atccggaagt cgatcgcgcg
      481 cgcgtcgcga tgcggcgagg tcggcgactc gccggcctgc acgcggtaca ccgacacgaa
      541 ctcgatcgtg cgcggctcgt acttctcggc gaggtcggcg agcatcgcca gggtccggcg
      601 cgcgatcgcg tgctggcggc cggtggtccg gcagcggaac aggtgcttga cctcggccgc
      661 ggtcgcgtcg tccatcgtgc cgtcgcgccg gatcccgacc gtagcgctga cccgcaggtt
      721 ctcgtcgtag agcttgacct cgatcggcgc ggccgcgcgc tcggcgacgg tcatcaccag
      781 cgcatcgacc ggcacggccc ccgggccggc ggcggcggcg agcccggcag cggccgcggt
      841 ggcgagcttg agcttgagaa tgatgggcag catcgttccc tcgtgcaccc acccggtgcg
      901 tgagaacctt cgtacgagcc gcggacgctt gcaactcaaa tgatccatct gaccctcacg
      961 cagccgcctt cgtggacatc acgccgcagg ccgcgcgctg cacatccggt atttccgccc
     1021 cgcagaacgc aatttcacgc ggttcgctgc gaacgctttg gcgcgcgcgt ggcctgttgt
     1081 acgttctctg cgcatgaagt tcaccgagga acacctacag ctccgccgca ccgtccgcga
     1141 gttcgtggac aaggagatca acccgcacta cgacgcgtgg gaggccgccg gcgcgttccc
     1201 ggcgcacgag gtgttcaaga aggccggccg gctcggcctg ctcggcgtca acaagcccga
     1261 ggcctacggc ggcctcggcc tcgactactc gtaccagatg tgcgtcatcg aggagctcgg
     1321 cacctgccgc ggcggctcgg tcacgatggc gctcggcgtc cagatggaca tggcgacccc
     1381 ggcgctggcc cggtggggca gcgacgagct gcgccgcgag ttcctcgtcc cggcgatcgc
     1441 gggcgacgtc gtcacgtcga tcgcggtgag cgaggtgtcg ggcggctccg acgtcgcggc
     1501 gatcaagacc accgcgcgca aggacggcgc cgactacgtc atcaacggct cgaagatgtg
     1561 gatcaccagc gcggcgcagg ccgactggct gtgcctgctg gtcaacaccg gcgacggctc
     1621 gccgcaccgc aacaagacgc tggtcatcgt gccgaccaag ctgcccggcg tgacgatcgg
     1681 ccccaagatc gacaagctcg ggatgcgggc cagcgacacc tgcccgatct acttcgagga
     1741 cgtccgcgtg ccccagcgcc accgcatcgg cgaggagggc atggggttca tgatgcagat
     1801 ggtgcagttc caggaggagc gactcaacgg cgcgatcagc tcggtgcgcg gcatggaccg
     1861 gacgctcgat gcgacgatcg cctactgcaa ggagcgccac accttcgggc aaccgctgat
     1921 cgacaaccag gtgatccact tccggttcgc cgagctcaag accgagatcg aggcgctgcg
     1981 ctcgctggtc taccgcgcgg tcgaggacta cgtcggcggc gccgacgtca cgatgatggc
     2041 gtcgatggcc aagctgaagg ccggccggct cgcccgcgag gtgtccgacg cctgcctgca
     2101 gtactggggc ggcatgggct tcacctggga caacccggcg gcgcgcagct accgcgatag
     2161 ccggctctta tcgatcggcg gcggggcgga cgagatcatg ctcggcatca tctgcaagtt
     2221 gatgggcatc ctgccgggca agaagaagaa gtaacgctac atatagaggc agtgcgagct
     2281 gccctggtga ccgcctgcgc gttgctcggc gcggtcg
//
LOCUS       DASVOR010001016         1912 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_272932,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001016 DASVOR010000000
VERSION     DASVOR010001016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1912
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272932"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..999)
                     /locus_tag="VF516_19660"
     CDS             complement(<1..999)
                     /locus_tag="VF516_19660"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX8109962.1"
                     /translation="MLSQPEAHRLVDRLIALVRATKGADALVTLRSSREGNTRFAVNE
                     ISTSGDVERLSIALTVQLGQRSATAVTNQQDDRSIDELVGRTLRMARLAPENPEDMPP
                     LGHQSYAPARNAADPATVKLAPDVRAKAVGAALAAGQAGNVVMAGFVNHASHSLAIGT
                     SAGLWAYHTWTSCGMSCTARTPDATGSGWAGAGSNRMADLDAGAIAKTAADKANASAR
                     AHKLEPGRYTVILEPAAVASLLAFLTNALDARRADEGRSYFSKHRPGDKLFPETITLR
                     SDPGDAALGALPFDAEGFPLTPNRWVDKGVLTALPYSRYWAKKQGKQPTGRVGGGGP"
     gene            complement(1002..>1912)
                     /locus_tag="VF516_19665"
     CDS             complement(1002..>1912)
                     /locus_tag="VF516_19665"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8109963.1"
                     /translation="RRGFESCNAEIPPRQAGWEYIESIPMVADARRVAEAAVEKLKAP
                     SVAPGKKDLILDPSNMWLTIHESIGHSTELDRALGYEANYAGTSFATPDKRGKLRIGA
                     PILTFFADKTTPGGLATCGYDDDGVATQRWNLVENGVFVNYQTTREQAGWIGEAASRG
                     TCYADSYGSFPFQRMPNVSLAPGPKERSIDDLIAMTDDGVVIVGNGSWSIDHQRFNFQ
                     FGGQMFWEVKRGKKTRMLRDVAYQANTIEFWNSCDAIGGPNSWWLGGSLSDGKGEPGQ
                     VNSVSHGCPPARFKQVNILTTNRRTA"
BASE COUNT          236 a          651 c          713 g          312 t
ORIGIN      
        1 cgggccaccg ccgccgacgc gaccggtcgg ctgcttgccc tgcttcttgg cccagtaccg
       61 cgagtacggc agggcggtga gcacgccctt gtcgacccac cggttcggcg tgagcgggaa
      121 gccctcggcg tcgaacggca gcgcgcccag ggcggcatcg cccggatcgc tgcgcagcgt
      181 gatggtctcg gggaacagct tgtcgcccgg ccggtgcttg ctgaagtacg agcggccctc
      241 gtcagcgcgc cgcgcgtcga gcgcgttggt caggaacgcc agcaggctcg cgaccgcggc
      301 gggctcgagg atcacggtgt agcggccggg ctcgagcttg tgcgccctgg ccgacgcgtt
      361 ggccttgtcg gctgcggtct tcgcgatcgc cccggcgtcg aggtcggcca tgcggttcga
      421 gccggcgccc gcccagcccg agccggtggc gtccggggtg cgcgcggtgc acgacatccc
      481 gcacgacgtc cacgtgtggt aggcccacag cccggcgctg gtgccgatcg ccaggctgtg
      541 gctggcgtgg ttgacgaagc cggccatcac gacgttgccg gcctggccgg cggcgagcgc
      601 ggcgcccacc gccttggccc gcacgtcggg cgcgagcttg acggtcgcgg ggtcggcggc
      661 gttcctggcc ggggcgtagc tctggtggcc gagcggcggc atgtcctcgg ggttctccgg
      721 ggcgagccgc gccatccgca gcgtgcggcc gaccagctcg tcgatcgagc ggtcgtcctg
      781 ctggttggtg accgcggtcg ccgagcgctg gccgagctgt acggtgagcg cgatcgacag
      841 ccgctcgacg tcgcccgacg tcgagatctc gttgaccgcg aaccgggtgt tgccctcgcg
      901 cgagctgcgc agggtgacga gcgcgtcggc gcccttggtg gcgcggacca gcgcgatcag
      961 ccggtcgacc aggcggtgag cttcgggttg actcagcatg atcaggctgt ccgtcggttg
     1021 gtggtgagga tgttgacctg cttgaaccgc gccggcgggc agccgtggct caccgagttg
     1081 acctggccgg gctcgccctt gccgtcggac agcgagccgc cgagccacca cgaattcggc
     1141 ccaccgatcg cgtcgcacga gttccagaac tcgatcgtgt tggcctggta ggccacgtcg
     1201 cgcagcatcc gggtcttctt gccgcgcttg acctcccaga acatctggcc gccgaactgg
     1261 aagttgaacc gctggtggtc gatcgaccac gagccgttgc cgacgatcac cacgccgtcg
     1321 tcggtcatcg cgatcaggtc gtcgatcgag cgctccttgg ggccgggcgc gagcgagaca
     1381 ttgggcatgc gctggaacgg gaagctgccg tagctgtcgg cgtagcacgt gccgcgcgac
     1441 gcggcctcgc cgatccagcc cgcctgctcg cgggtggtct ggtagttgac gaacacgccg
     1501 ttctcgacca ggttccagcg ctgggtcgcg acgccgtcgt cgtcgtagcc gcaggtcgcc
     1561 aggccgccgg gcgtggtctt gtcggcgaag aacgtcagga tcggcgcgcc gatccggagc
     1621 ttgccgcgct tgtcgggcgt ggcgaacgac gtgcccgcgt agttggcctc gtagccgagc
     1681 gcgcggtcga gctcggtcga gtggccgatc gactcgtgga tcgtcagcca catgttcgac
     1741 gggtcgagga tcaggtcctt cttgccgggc gcgaccgacg gcgccttgag cttctcgacc
     1801 gcggcctcgg cgacccggcg cgcgtcggcg accatcggga tcgactcgat gtactcccag
     1861 ccggcctggc ggggcgggat ctcggcgttg cacgactcga agccgcgccg gt
//
LOCUS       DASVOR010001017         1551 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_185804,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001017 DASVOR010000000
VERSION     DASVOR010001017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185804"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VF516_19670"
     CDS             <1..643
                     /locus_tag="VF516_19670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEX8109964.1"
                     /translation="STPAMPERRYLVKEMFGPTLQGEGAHAGRSVVFLRFAACNLACP
                     GCDTDFSPAGARRMAAGDIVAQLVALDTGCARRVVVTGGEPTLQWDAALAAAIRGAGF
                     RVHMESNATRPLAGPVDWLTISPKPQHHGTHAARVALADGLPANEVKVVVDDTVDEAV
                     LDRYAQRWRCDHYFLQPWMDERYDHHLARTLALIHARPRWRLSLQLHKIIGVP"
     gene            640..>1551
                     /locus_tag="VF516_19675"
     CDS             640..>1551
                     /locus_tag="VF516_19675"
                     /inference="COORDINATES: protein motif:HMM:NF022646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2891 domain-containing protein"
                     /protein_id="HEX8109965.1"
                     /translation="MNQDQASHFARLALGCIQRELPNGIQHTVNAPDDVRGPRALHPA
                     FYGCFDWHSAVHGHWMLVRLLRRWPDLPEAAAIRAGLGANLTAAHLAAEAAYFTAPNR
                     ASYERPYGWAWLLQLAAELHTWNDPDARCWAAHLQPLADLLAERYLAFFPKQTYPVRT
                     GVHSSTAFGFVLALDYARAVGHTDLEALICERSVAYYARDRDAPAAWEPSGEDFLSPS
                     LIEADLMRRVLPSAEFPAWLHAFLPRGLPPSLQQPPIVTDRSDGKLAHLDGLNLSRAW
                     CLRAIAAALPAGDPLRPPLTASADAHAA"
BASE COUNT          191 a          647 c          501 g          212 t
ORIGIN      
        1 tagcaccccg gcaatgccgg agcgccgcta tctcgtcaag gagatgttcg gccccaccct
       61 gcagggcgag ggagcccacg ccgggcggtc ggtggtgttc ctgcggttcg cggcgtgcaa
      121 cctcgcgtgc ccgggctgcg ataccgattt ctcgcccgct ggtgcccgcc ggatggcggc
      181 cggggacatc gtcgcgcagc tggtcgcgct cgacaccgga tgcgcccgcc gcgtcgtggt
      241 gaccggcggc gagccgacgc tgcagtggga cgccgcgctg gccgccgcga tccgcggcgc
      301 cgggttccgc gtccacatgg agtccaacgc gacccgcccg ctcgccggcc cggtcgactg
      361 gctcacgatc tcgcccaagc cgcagcacca cggcacccac gccgcgcgcg tcgccctcgc
      421 cgacgggctg cccgcgaacg aggtcaaggt cgtggtcgac gacacggtcg acgaggccgt
      481 cctcgaccgc tacgcgcagc gctggcgctg cgaccactac ttcctccagc cgtggatgga
      541 cgagcgctat gatcaccacc tcgcccgcac cctcgcgctg attcacgccc ggccgcgctg
      601 gcggctgtcc ctgcagctcc acaagatcat cggggtacca tgaaccagga ccaggccagt
      661 cacttcgccc gcctcgcgct cggctgcatc cagcgcgagc tgcccaacgg catccagcac
      721 acggtcaacg cgccggacga cgtgcgcggc ccgcgcgcgc tccacccggc gttctacggc
      781 tgcttcgact ggcactccgc ggtgcacggt cactggatgc tggtccgcct gctgcgccgc
      841 tggccggatc tccccgaggc cgccgcgatc cgcgccgggc tcggcgccaa cctgaccgcg
      901 gcccacctcg cggccgaggc cgcctacttc accgcgccca accgggccag ctacgagcgg
      961 ccgtacggct gggcctggct gctccagctc gccgccgagc tccacacctg gaacgacccc
     1021 gacgcccggt gctgggccgc ccacctgcag ccgctggccg atctgctcgc cgagcgctac
     1081 ctcgcgttct tccccaagca gacctacccc gtccgcacgg gagtccactc cagcaccgcg
     1141 ttcggcttcg tgctcgcgct cgactacgcg cgcgcggtcg gccacacgga cctcgaggcg
     1201 ctgatctgcg agcgctcggt cgcctactac gcccgcgatc gcgacgcgcc ggccgcctgg
     1261 gagccgagcg gcgaggactt cctgtcgccc tcgctgatcg aggccgacct gatgcgccgc
     1321 gtgctgccgt ccgccgagtt cccggcctgg ctgcacgcgt tcctgccccg cggcctgccg
     1381 ccgtcgctcc agcagccgcc gatcgtcacc gaccgcagcg atggcaagct cgcccacctc
     1441 gacggcctca acctgtcgcg cgcgtggtgc ctgcgcgcca tcgccgccgc gctgccggcc
     1501 ggcgatccgc tgcgcccgcc gctgacggcc tcggccgacg cccacgccgc c
//
LOCUS       DASVOR010001018         1824 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_127020,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001018 DASVOR010000000
VERSION     DASVOR010001018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1824
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127020"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..1433)
                     /locus_tag="VF516_19680"
     CDS             complement(153..1433)
                     /locus_tag="VF516_19680"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-binding family 9-like protein"
                     /protein_id="HEX8109966.1"
                     /translation="MRIALVAGLLAWVACVDRGPGPQPKKIDPAYVAAHLLAQPPAGL
                     TPRLDVALGGGKVIYLGNTVDRAAVVPGQIVRVTHYWQVVQPIGTGWKVFARVRGAPG
                     TADFINLPATDMELGHDPARWQPGEVIEDVQDITVRPDWRSPTATLTVGLIAVGQHGV
                     GDRMAASGPHTQDRAVIARVIDVDLSKAPPPPGTVHVPRALGPIAIDGVASEPAWATA
                     ATSPELVTAEGSADPVGKAIARLTWDDQYLYAFVSITDSDIVSEYKNHDEPLWKADCV
                     ELFIDADGNRRGYIELQVNPNNATFDSWFAVTRAQGGDPSWDSGMQTAVKLRGTTASG
                     DTDQGWDAEIAIPWQAVRGRDAAMAVRLPPEVGDRWRMNLVRVDVRTGDKNPQASSWN
                     RISSADFHALDRMLTVVFADPSGSIAPQPAGEPR"
     gene            complement(1505..>1824)
                     /locus_tag="VF516_19685"
     CDS             complement(1505..>1824)
                     /locus_tag="VF516_19685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109967.1"
                     /translation="RPGAPVDARDPGEQAGNEPPMPPGQSGASMGAQGQGGALPPAPP
                     PGGSDEPKDDPVYKKWWFWGILAVTGVVVYELATSPSSNNTARIQPPKAAAQPGGLTL
                     LRW"
BASE COUNT          229 a          666 c          670 g          259 t
ORIGIN      
        1 acgcggcggc cccgcgcacg cccccagcaa cccgcatgcg accgcgacgt atcgcatcgc
       61 cggcgatcgt accacggtgc cgtgcggcag cagccggcga gatctggcag ctgcgttccg
      121 tccgcgaacc gcgcgcgcgt cccgtgtcgc agctatcgcg gctcgcccgc cggctgcggc
      181 gcgatcgacc cgctcgggtc ggcgaacacc acggtcagca tgcggtcgag cgcgtggaag
      241 tcggccgacg agatccggtt ccacgacgac gcctgcgggt tcttgtcgcc ggtccggaca
      301 tcgacccgga caaggttcat ccgccagcgg tcgccgacct cgggcggcag ccgcaccgcc
      361 atcgccgcgt cgcggccacg caccgcctgc cacgggatcg cgatctcggc gtcccacccc
      421 tgatcggtgt cgccggacgc ggtcgtgccg cgcagcttga ccgcggtctg catccccgaa
      481 tcccagctcg gatcgccgcc ctgggcgcgg gtcaccgcga accacgagtc gaacgtggcg
      541 ttgttggggt tgacctggag ctcgatgtag ccccggcggt tgccgtcggc gtcgatgaac
      601 agctcgacgc agtcggcctt ccacagcggc tcatcgtggt tcttgtactc gctcacgatg
      661 tcgctgtcgg tgatcgacac gaacgcgtag aggtactggt cgtcccaggt cagcctggcg
      721 atcgccttgc cgaccggatc ggcgctgccc tcggcggtga ccagctcggg tgaggtcgcg
      781 gcggtggccc acgccggctc ggaggcgacc ccgtcgatcg cgatcggccc gagcgcgcgc
      841 ggcacgtgga cggtgccggg cggcggcggc gccttggaca ggtcgacgtc gatcacgcgc
      901 gcgatcaccg cgcggtcctg ggtgtgcggg ccgctcgccg ccatgcgatc gccgaccccg
      961 tgctggccga ccgcgatcag cccgacggtg agcgtcgcgg tcggcgagcg ccagtccggg
     1021 cgcacggtga tgtcctggac gtcctcgatc acctcgccgg gctgccagcg cgccggatcg
     1081 tggccgagct ccatgtcggt cgcgggcagg ttgatgaagt cggccgtgcc gggcgcgccg
     1141 cggacccgcg cgaatacctt ccagccggtg ccgatcggct ggacgacctg ccagtagtgg
     1201 gtgacgcgca cgatctggcc ggggaccacc gcggcccggt cgaccgtgtt gccgaggtag
     1261 atcaccttgc cgccgccgag cgccacgtcg agccgcggcg tcaggccggc gggcggctgc
     1321 gccaggaggt gcgcggccac gtacgccgga tcgatcttct tcggctgcgg cccggggccc
     1381 cggtcgacgc acgcgaccca ggcgagcaag ccggcaacga gcgcgatgcg catcggctgg
     1441 aatgtacgat gcaccggccg cgcggggtca ccggccggcc gggaatgtca ggcgctggcc
     1501 ccggtcacca gcgcagcagc gtcagcccgc cgggctgcgc ggcggccttg ggcggctgga
     1561 tgcgagccgt gttgttcgac gacggcgagg tcgcgagctc gtagacgacg acgccggtga
     1621 ccgcgaggat cccccagaac caccacttct tgtagaccgg gtcgtccttg ggctcgtcgg
     1681 agccacccgg cggcggggcc ggtggcagcg cgccgccctg gccctgggct cccatgctcg
     1741 cacccgattg cccgggaggc atcgggggct cgttgccggc ttgctcgcct gggtcgcgtg
     1801 cgtcgaccgg ggccccgggc cgca
//
LOCUS       DASVOR010001019         3458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448413,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001019 DASVOR010000000
VERSION     DASVOR010001019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448413"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VF516_19690"
     CDS             <1..928
                     /locus_tag="VF516_19690"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8109968.1"
                     /translation="AGKVGAGFIDAGIGGGVESMSRVPMGSDEAMIDGLNLKLRERLF
                     MVPQGISADLIATREGFTRDDVDRFALASQQKAARAIEGKRFDSALFPVKNADGSVAL
                     ARDEHPRADTSLESLAQLQPAFEKLGAAPMGSRGETVDQLALKRYPDTRKIEHVHTAG
                     NSSGIVDGAAVVLLGSAEWGKSAALKPRARIRAMATAGAEPLIMLTAPAPASAKALKK
                     AGMTVKDIDLWEINEAFAVVPLQTARALGIDPDRINVNGGAIALGHPLGATGAILLGT
                     ALDELERTGKTTALITLCIGGGMGIATIIERV"
     gene            complement(953..2329)
                     /locus_tag="VF516_19695"
     CDS             complement(953..2329)
                     /locus_tag="VF516_19695"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8109969.1"
                     /translation="MGSSEAELVAVYGRRRVGKTYLIRELLRDAICFELTAVYGASVR
                     DQLANFATALAVTRGSPLDPPSSWRAAFEQLIATLNQRSSRKRVVFFDELPWLSSRRS
                     GFLPAFEHFWNSWAVKQRDLVVVVCGSAASWMIHQLLSARGGLHNRVTRRIRLEPFTL
                     SETEEYFASRSMNLGRYQILELYAAFGGVPYYLKQVERGDSASVAIDRICFARDGALR
                     DEFPKLYASLFEHSDRHVRLVKALGAKPRGLIRNELLDAAELTSGGGASRILDELEES
                     GFILRTPQLELPIKDAVVRLIDEYSLFYLKWIERHRGSADHVWINKRGTPAWRAWSGY
                     AFEGICIKHVASLKRALGIEAVETTESAWHHRPIGRDDRGAQVDLVIDRRDASVNLCE
                     MKFSEGEFSIDRNYAAELRRKRDTFRRITGSRKTLLITMVTTHGLRSNQYARDLVDKA
                     LTLDALFR"
     gene            complement(2665..3198)
                     /locus_tag="VF516_19700"
     CDS             complement(2665..3198)
                     /locus_tag="VF516_19700"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8109970.1"
                     /translation="MTTTTYELDPVHSHLGFSVRHMMVSQQRGQFQGVTGTLTLDRED
                     ITRSHVEVSIDVATINTNNTDRDNHLRSADFFDVANHPKMTFVSRDVKAQPDGELRIT
                     GDLTIRGTTRSVTLTAEPLSPESKDPFGNIKIGTSATGKLSRKEFGMVWNAVLETGGI
                     ALADEVKLTLDVQFVRK"
BASE COUNT          546 a         1139 c         1216 g          557 t
ORIGIN      
        1 cgccggcaag gtcggcgccg ggttcatcga cgcgggcatc ggcggcggcg tcgagtcgat
       61 gtcgcgggtg ccgatgggct ccgacgaggc gatgatcgac ggcctcaacc tcaagctgcg
      121 cgagcggctg ttcatggtgc cccagggcat ctcggccgac ctgatcgcga cccgcgaggg
      181 cttcacgcgc gacgacgtcg accgcttcgc gctcgccagc cagcagaaag ccgcgcgcgc
      241 gatcgagggc aagcggttcg acagcgcgct gttcccggtc aagaacgccg acggctcggt
      301 cgcgctcgcc cgcgacgagc acccgcgcgc cgacaccagc ctggagtcgc tcgcccagct
      361 ccagcccgcg ttcgagaagc tcggcgcggc gccgatgggc agccgcggcg agaccgtcga
      421 tcagctcgcg ctcaagcgct accccgacac caggaagatc gagcacgtcc acaccgccgg
      481 caactcgtcg ggcatcgtcg acggcgccgc ggtcgtcctg ctcggctcgg ccgagtgggg
      541 caagtccgcc gcgctcaagc cgcgcgcccg gatccgcgcg atggccaccg ccggcgccga
      601 gccgctgatc atgctgaccg cccccgcccc cgcgtcggcc aaggcgctca agaaggccgg
      661 catgacggtc aaggacatcg atctgtggga gatcaacgag gcgttcgcgg tggtcccgct
      721 gcagaccgcc cgcgcgctcg gcatcgatcc cgaccggatc aacgtcaacg gcggcgcgat
      781 cgcgctcggc cacccgctcg gcgcgaccgg cgcgatcctg ctcggcaccg cgctcgacga
      841 gctcgagcgt acgggcaaga ccaccgcgct gatcacgctg tgcatcggcg gcggcatggg
      901 gatcgcgacg atcatcgagc gcgtgtagcc ggcggtcgtc cgccaccggc gatcacctga
      961 acagcgcgtc gagcgtcaac gccttgtcga ccagatcgcg cgcatactgg ttgcttcgca
     1021 agccatgcgt ggtgaccatc gtgatgagta gtgtcttgcg agagccggtg atgcggcgga
     1081 aggtgtcgcg cttgcggcgc agctccgctg cgtagttcct gtcgatcgag aattcgccct
     1141 cggagaactt catctcgcac aggttgacgg atgcatctcg acgatcgatg acgagatcga
     1201 cctgcgcgcc gcgatcgtcc cgaccgatcg gacgatgatg ccacgcgctc tcggtggtct
     1261 cgactgcctc gatgcccagc gcgcgcttga gcgacgcgac gtgcttgatg cagatgcctt
     1321 cgaacgcgta gccgctccat gcgcgccatg ccggcgtccc gcgcttgttg atccacacgt
     1381 gatcggcgct gccgcggtga cgttcgatcc acttgagata gaacagggaa tactcatcga
     1441 tcagccgcac cacggcgtcc ttgatcggga gctccagctg cggagtgcgc aggatgaagc
     1501 cggactcctc cagctcgtcc aggatccggc tggctccccc gccggaggtc aactcggccg
     1561 cgtcgaggag ctcgttgcgg atcagcccgc gcggcttggc gccgagcgcc ttcacgagcc
     1621 gaacgtgtcg gtcggagtgc tcgaacagcg atgcatagag cttcgggaac tcatcgcgga
     1681 gcgcgccgtc gcgcgcgaag cagatccgat cgatggcgac gctggcagag tcaccgcgtt
     1741 cgacctgctt gaggtagtag ggcacacctc cgaacgcagc gtagagctcg aggatctggt
     1801 agcggccgag gttcatcgat cgtgacgcga agtactcctc ggtctccgat agcgtgaacg
     1861 gctccagccg gatccggcgc gtgacacggt tgtgcagccc accgcgcgcg ctcagcagct
     1921 ggtgaatcat ccaggaagcg gccgatccgc acacgaccac gaccagatcg cgctgcttca
     1981 cggcccacga gttccagaag tgctcgaacg cgggaaggaa accggaacgg cgcgacgaca
     2041 gccaggggag ctcgtcgaag aacaccaccc gctttcgcga ggatcgctgg ttcagcgtcg
     2101 cgatgagctg ctcgaaggcg gctcgccagc tcgacggcgg atccagcggt gatccccgag
     2161 tgaccgcgag cgccgtggca aagttcgcga gctggtcgcg gaccgaggct ccgtacacag
     2221 ccgtcagctc gaagcagatc gcgtctcgga gcagctcgcg gatcagatag gtcttcccga
     2281 ctcgcctccg cccgtacacc gcgaccagct cggcctccga ggaacccatc acctgggcga
     2341 agagagctcg ctcgcgtgtg cgtccgacca gggccacccg ctctatttgg ccgaaacccg
     2401 gcctgatgac aaggtttcgg ccagatagtg gcgctatttg gccaaaactt gggtccacag
     2461 gtgggtttcg gccagatagt ggcgctattt ggccaaaacc ggggtcccgg gatgggtttc
     2521 ggccagatag cggcgctatt tggccaaaac cggggtccgg ggtgggtttc ggccagatag
     2581 tggcggctgc gccctgcagc cgcgagcgca ggtcgtcgag agcgatcggc ggtcggcgga
     2641 cgctgtccgg ccgcgcggcg gtcgctactt gcgcacgaac tggacgtcga gcgtcagctt
     2701 gacctcgtcc gcgagcgcga tgccgccggt ctcgagcacc gcgttccaca ccatgccgaa
     2761 ctccttgcgg ctgagcttgc cggtcgccga ggtgccgatc ttgatgttgc cgaacgggtc
     2821 cttggactcg ggggacagcg gctcggcggt cagggtgacc gagcgggtcg ttccgcggat
     2881 cgtgagatcg ccggtgatgc ggagctcgcc gtcgggctgg gccttcacgt cgcgcgacac
     2941 gaaggtcatc ttggggtggt tggcgacatc gaagaagtcg gcgctgcgca ggtggttgtc
     3001 gcggtcggtg ttgttggtgt tgatggtcgc gacgtcgatc gagacctcga cgtggctcct
     3061 ggtgatgtcc tcgcggtcga gcgtgagggt gcctgtcacg ccctggaact ggccgcgctg
     3121 ctgcgagacc atcatgtgtc gcacgctgaa gccgaggtgg ctgtggacgg ggtcgagctc
     3181 gtaggtcgtg gtggtcatgg cgcgctattt gaacacgaac gcgcgtcgac tgtgaggcgg
     3241 ccgcatggga cgctccgtcc ggcggcgcgg gacgcctgtc cgtcggctcg tggtagggtc
     3301 cggccagtgc accgctggct cgcgcttgtg gcgctcgcgt cgtgcgagct gaccgcgccg
     3361 ccgcccgagg ccgggaccga ggatcgggcg gggccagtgt gggtgagccc gccggggccg
     3421 ggcgggtttc cgctgggagc gatccgcggg cggctggg
//
LOCUS       DASVOR010001020         2737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_272972,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001020 DASVOR010000000
VERSION     DASVOR010001020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272972"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..559)
                     /locus_tag="VF516_19705"
     CDS             complement(83..559)
                     /locus_tag="VF516_19705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109971.1"
                     /translation="MTRRFDPMERRNADLFLADLMGRGARPFEDQGEAAEPDIGSSGR
                     EPFFGDKRGEWWRVIARPGARLRWLHLRDGDLIVRRMSAESGQGWQCLLKEDVDPETL
                     RGPEGRLSDDTVVLRRVSRWGRATEREAIDEGYAGEPAEDDPPTGWAGGSAGGRHG"
     gene            1227..>2737
                     /locus_tag="VF516_19710"
     CDS             1227..>2737
                     /locus_tag="VF516_19710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109972.1"
                     /translation="MRGSVEQGDTGAGLAGLRVELWRSTDRGAERVAVTRSDDAGLFS
                     LDLPAGLDGDGDGYGTGPFEVELRVLEGGRLLLSEPREVVLAGDLEPIELCVPRNPED
                     AGALAGDAIESQDGGGRAEVWGRVEGVVPEDAVVEAVLRTLSGGSAIERRVVGQAAPN
                     EAGWFRVRYERSGGGGRSPDAAISVRLRGADGSLLAETAPVLARAPRLRMDLGPVTGR
                     SGPSEYILIERRLAGGLGAGAAAVEALDGEELSEVATWIEVEPERLALFRQARALEAE
                     TGLPGALFYGLGRGGLGMTLESLADVPLHVVETTIKEAVADGVTCQTLLPDLDPLLER
                     LASEVVDHVLRADRAPVPAGRLGDVLSAAGLPGETVRRVLRSHQSWTGPAAEFWDSLT
                     EGDSTAEGLDEDAGRETRLAVELADLLGPDPALLRRVYELRADGRWQAPQDLASLGVD
                     DWRQLLESTAVRAGGAGEADEEDAEAQDEIHARAEGIVESLEKAFPSAFIRRR"
BASE COUNT          345 a          976 c         1035 g          381 t
ORIGIN      
        1 gccccggtct cgaggcgcag gatcaggctg acctcctcgt cgtcggggcc agcttcgttc
       61 agctcgttga gctcggggtc catcatccgt gcctcccgcc cgcggatccg ccggcccatc
      121 ctgtgggcgg gtcgtcctcc gccggctcgc cggcgtaccc ctcgtcgatc gcctctcgct
      181 cggttgcacg cccccaccgc gacacccgcc gcagcacgac cgtgtcgtcg ctcagccggc
      241 cctcgggccc gcggagcgtc tcggggtcga cgtcctcctt gagcaggcac tgccagccct
      301 ggccgctctc ggcggacatg cggcgcacga tcagatcgcc gtcgcgcagg tgcagccagc
      361 ggaggcgcgc gcccggccgc gcgatgaccc gccaccactc gccgcgcttg tcgccgaaga
      421 acggctcgcg gcccgaggag ccgatgtcgg gctcggcggc ttcgccctgg tcctcgaacg
      481 gccgcgcgcc gcggcccatg aggtcggcca ggaacagatc cgcgttgcgc cgctccatcg
      541 ggtcgaatct gcgtgtcacg tcgtttcctc cacgtgccag agctcggtca cacgatgcac
      601 gcggttccgt ccccgaagac gtccagccgc cgcggtgccg ccgcggcgcc gcccagcagg
      661 tcggccagga accgatcggc attccggcgc gcgcggaggg acgccggcgg gagaccggct
      721 cgcccgccga ggaccgtact cccgtcccgc ggattccatc tgccgttcac ggggtcactc
      781 ccgccaggag gcggccggcc gacccggaaa gaggacgatg gacgctcgtg cgctcccgcg
      841 gagacgcgcg gtcgactccc cgatcgactt ccacctcggc catccgtgga cactcctctt
      901 caccgcgacc tgccgccgga caagcgcgtg cgaggcgcag gcggcggcaa cgtagcacga
      961 ggccgcgagc gcctcgcacg cgcgatgccc aaatcgtggg aagcgatttc actttttggg
     1021 tggacaggca ttccatcgaa ggcgcgccgc cgcactggcg cgctcccgtt accgcattcg
     1081 cgcacattat caacatcagt gaaacgcaat gattgcgcag caatgattgc gcagccatca
     1141 ttgcgcagcc cgcgttgtga tccgtgcgag cagcgcagta atgtgtgccc gggaggtctg
     1201 ctttgagcgt cggcgaggcg gtctcgttgc ggggttctgt cgagcagggc gacacgggcg
     1261 ccggtctggc cggtctgcgc gtggagctct ggcgatcgac cgatcgcggt gcggagcgcg
     1321 tggccgtcac ccgcagcgac gacgccggcc tgttctcgct cgacctgccg gccggcctgg
     1381 acggcgacgg cgacggctac gggacggggc cgttcgaggt cgagctgcgc gtcctcgagg
     1441 gcggacggct gctgctcagc gagccgcgcg aggtggtgct ggccggcgat ctggaaccga
     1501 tcgagctctg cgttccgcgc aatccggaag acgccggcgc cctggccggc gacgccatcg
     1561 aatcccagga cggcggcggg cgcgccgagg tgtgggggcg tgtcgaaggc gtcgtgccgg
     1621 aggacgcggt ggtcgaagcc gtgctgcgca cgctgtccgg cggttccgcc atcgagcgcc
     1681 gcgtggtggg ccaggccgct cccaacgagg cggggtggtt tcgcgtgcgc tacgagcgct
     1741 ccggcggggg cgggcgatct cccgacgccg ccatctcggt gcggttgcgc ggggccgacg
     1801 ggagcttgct ggccgagacc gcgcccgtgc tggcgcgcgc gccgcgcctg cgcatggacc
     1861 tcggccccgt gacggggcgg tccgggccgt cggagtacat cctcatcgag cgccggctgg
     1921 cgggcgggct cggcgcgggc gcggcggccg tggaggcgct cgacggcgag gagctgagcg
     1981 aggtcgcgac gtggatcgag gtcgagcccg agcgcctcgc gctgttccgg caggcccgtg
     2041 cgctggaggc cgagaccggt ctgcccgggg cgctgttcta cgggctgggc cgcggcgggc
     2101 tgggcatgac cctcgagtcg ctggccgacg ttccgctcca cgtcgtggaa acgacgatca
     2161 aagaggcggt ggccgacggc gtcacgtgcc agacgctgct gccggacctg gacccgctgc
     2221 tggagcgcct ggcctcggag gtcgtcgatc acgtgctccg cgccgaccgc gcgccggtgc
     2281 cggcggggcg cctgggcgac gtcctgtcgg cggcgggtct gcccggcgag accgtgcgcc
     2341 gcgtgctgcg cagccaccag tcgtggaccg gaccggcggc ggagttctgg gacagcctga
     2401 ccgagggcga cagcaccgcc gagggcctcg acgaggatgc cggccgcgag acccggctgg
     2461 cggtcgagct ggcggacctg ctgggcccgg atccggcgct gctccgccgg gtgtacgagc
     2521 tgcgggccga cgggcgatgg caggccccgc aggacctggc cagcctgggc gtcgacgact
     2581 ggcgccagct gctggaatcg acggcggtgc gcgccggcgg cgccggcgag gccgacgagg
     2641 aggacgcgga ggcgcaggac gagatccacg cgcgcgccga ggggatcgtc gagtcgctgg
     2701 agaaggcatt tccgagcgcc ttcatccgcc gccgcat
//
LOCUS       DASVOR010001021         3769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448431,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001021 DASVOR010000000
VERSION     DASVOR010001021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3769
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448431"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VF516_19715"
     CDS             <1..511
                     /locus_tag="VF516_19715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEX8109973.1"
                     /translation="SVPRTVPSRTPEDRVEVIAALRRLRFTGAQIAEVLGMPETTVSG
                     ILQRIGMGRLGRLGLEPAHRYERARPGELIHIDVKKLGRIHGGAGKRVTGVKRNPSKT
                     RRDKVAGFDRKTIGWEYVHIAIDDATRLAYVEVLADEKALTAVGFLRRAISHYASYGI
                     TVERLITDG"
     gene            complement(515..1861)
                     /locus_tag="VF516_19720"
     CDS             complement(515..1861)
                     /locus_tag="VF516_19720"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX8109974.1"
                     /translation="MRGFGLDVHRDFCEVAIAENGRIRSAGRIPTRVASLELFAQSLV
                     AGDVVALEATTGADKIVSILQRRGVRVVVANTRKLKSISQAKAKTDRLDARTLARLLM
                     SGLLDEVWTPDERTRTLRRLTNRRERVVRARTRAKNEAHGVLARNLCDRPPVTDAFGK
                     AGRRWLAELELPIDERLTLDGCLRQVDFLDSEIAALDREIAKHALAWPEVLRLMTVPG
                     VNVQTAATFMASVGDIRRFSSPRKLVSYLGLDPRVRQSGNAPARHGRISKAGASEARH
                     MLGEVAWKVMQTPGPLRAFFQRVRARRGPQIAATATARKLVVLFWHLLTRGEDYAFGR
                     PAMTRNKIRRLELLAGAPSQKGRKGIAGGKSKAVFDAERELSRQAEAAYRRLVSDWQA
                     TGPTKAGAGATPGRASQRSIKDKAARQTPQPQGLRFASSVTRTRPAGSHHPPTAAT"
     gene            2087..2191
                     /locus_tag="VF516_19725"
                     /pseudo
     CDS             2087..2191
                     /locus_tag="VF516_19725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011695115.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
     gene            2484..3533
                     /locus_tag="VF516_19730"
     CDS             2484..3533
                     /locus_tag="VF516_19730"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX8109975.1"
                     /translation="MVGIDVHKHTHAAALIDDRGGQVAAITVANSPKGYRRLIDWLVE
                     HDAAGAVIGVESPGSYGRCLVAALCGAGFEVLQVPAWRTHRERHHRGPGKTDPGDALS
                     IAHVVLRHRDELGPAAEPELVRALGMLELQRRRFVRDRTQAIQRLRSDWTQHDPVAEA
                     SVVRCDRQRELRKLKRIDLGDSLAHRIAARCIRQLANDIEDLNDKIAALDAEIAELLA
                     EHDNPFEDLLGAGTNLAATIIAQAGDVRRFRDASAFARFCGAAPIPCGSGQTSGRHRL
                     HRGGNRQLNAALYRIAIVQQRHHPAAKTYLARKIAEGKTAREARRALKRHLANVVYRR
                     LLTWANTTPALTDLT"
     gene            3529..3735
                     /locus_tag="VF516_19735"
                     /pseudo
     CDS             3529..3735
                     /locus_tag="VF516_19735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297375.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT          593 a         1384 c         1253 g          539 t
ORIGIN      
        1 ctcggtgccg agaacggtgc cctcgcgcac gcccgaggat cgcgttgagg tcatcgccgc
       61 gttgcgcagg ctgcggttca cgggcgccca gatcgccgag gtgctgggga tgcccgagac
      121 gacggtgtcg gggatccttc agcggatcgg catgggcagg ctgggccgcc tgggcctgga
      181 gcccgcgcac cgctacgagc gagcgcggcc cggcgagctg atccacatcg acgtcaagaa
      241 gctcgggcgc atccacggcg gcgcgggcaa gcgcgtcacc ggtgtcaagc gcaacccctc
      301 caaaacccgc agagacaagg tcgccggctt tgaccgcaag accatcggct gggagtacgt
      361 gcacatcgcc atcgacgacg ccacccgcct ggcctatgtc gaagtgctcg cagacgaaaa
      421 ggccctcacc gccgtgggct tcctgcgccg cgccatcagt cactacgcca gctacggcat
      481 cacggtcgag cgcttgatca ccgacggatg aaagtcaagt ggcggcggtc ggcgggtggt
      541 gcgatcctgc ggggcgggtg cgggtgactg acgaagcgaa gcgcaggcct tggggctgtg
      601 gagtctgccg cgcggctttg tcctttatgg acctctgaga tgcgcgcccc ggtgtcgcgc
      661 ccgcacccgc cttggtcggt ccggtggcct gccagtcgct gaccaggcgg cgataggcgg
      721 cttcggcctg gcgggagagt tcgcgttcgg cgtcgaagac ggccttggat ttgccgccgg
      781 cgatgccctt gcggcccttc tgtgagggcg cgccggcgag cagctcgagg cggcggatct
      841 tgttgcgggt catcgctggg cgcccaaagg catagtcctc gccgcgggtg agcaggtgcc
      901 agaagaggac gacgagcttt ctggcggtgg cggtcgcggc gatctggggg ccgcggcgcg
      961 cacgcacgcg ttggaagaac gcgcgcagtg gccccggggt ctgcatgacc ttccaggcga
     1021 cctcgccgag catgtggcgg gcctcggagg caccggcctt ggagatccgg ccgtggcgag
     1081 cgggggcgtt gcccgactgg cgcacccggg ggtccaggcc cagatagctg accagcttgc
     1141 gcggcgagga gaagcggcgg atgtcaccga ccgaggccat gaacgtcgcc gcggtctgga
     1201 cgttgacgcc gggaaccgtc atcagccgca acacctccgg ccacgccagc gcgtgcttgg
     1261 cgatctcgcg atcgagcgcc gcgatctcgc tgtcgaggaa gtcgacctgg cgcaggcacc
     1321 cgtcgagcgt caggcgctca tcgatcggca gctccagctc ggccagccag cgccggccgg
     1381 ccttgccgaa ggcgtcggtg accggcgggc ggtcgcacag gttgcgcgcc agcacgccgt
     1441 gcgcctcgtt cttggccctg gtccgggcgc gcacgacccg ttcgcggcgg ttggtcagcc
     1501 gccgcagcgt gcgcgtccgt tcatcgggcg tccagacctc gtccagcagc ccggacatca
     1561 gcagcctggc aagcgttctg gcgtccagcc gatcggtctt ggccttggcc tggctaatcg
     1621 acttgagctt gcgggtgtta gcgaccacca cccgcacccc gcggcgctgc aggatcgaca
     1681 cgatcttgtc agcgccggtc gtcgcctcca gcgcaaccac atcgcccgcc accaggctct
     1741 gggcgaacag ctcaagcgac gcaacccgcg tcgggatccg ccccgccgac ctgatcctcc
     1801 cgttctcggc gatcgcgacc tcgcagaagt cgcgatgcac atccagtccg aatccgcgca
     1861 ccagccctcc tctcgattga ccttgggcgg gagcgcggcg ggacacacga cagcgacgga
     1921 ttcgtgctct cagcacaccc ggacaagtcg caggggcggc cacgaataac gtcgagctca
     1981 cggctcatca gtcagagcgg cctgcccgac cgcgtctccc aacagcgccc cttgttccgg
     2041 gaaccgacag gctcccggca gcgccggacg ggcagtttca tgtccggata acggctcggc
     2101 ctatcgctcc gccgtgcacg cgatcgcctg ccgcacgctg gggatccgcc acctgcgcac
     2161 gcggccccac cgcccgcaga ccaacggcaa gaatctgtgt cttagcgtgg ccgggccgtc
     2221 cggtggggcg gcgaggctcc ccgcgcctgt cggtctgact caggcaaaag gcatgccgat
     2281 ggtgggaagc gtggtcggtc ccggccggga ccggccacgc tggccgccga ctggcctgcg
     2341 ttcgatttgt cggccggcgg gttgcgggcg ggccggcgaa gctgacttga taggagaact
     2401 cgcgccgatg tggctgcatc gacacagaag cgtcgctcgc cggcccggcc tcgactctat
     2461 ctgcgagcca ggaggccacc ttgttggtcg gcatcgacgt tcacaagcac acccacgccg
     2521 ccgcgctgat cgatgatcgc ggcgggcaag tcgcggcgat caccgtcgcc aacagcccca
     2581 agggctatcg gcgtctgatc gattggctgg tcgagcacga cgccgcaggg gcggtgatcg
     2641 gcgtggagag ccccggcagc tatggacgct gcctggtggc cgcgttgtgt ggcgctgggt
     2701 tcgaggtcct gcaggtcccg gcatggcgca cccatcgcga gcgccaccac cgcgggcccg
     2761 gcaagaccga ccccggcgac gcgctgtcga tcgcgcacgt cgtgctcaga caccgcgacg
     2821 aactcggccc cgccgccgag cccgagctcg tgcgcgcgct gggcatgctc gagctgcagc
     2881 gccgacgctt cgttcgcgat cgcacccaag cgatccagcg cctgcgctcg gactggaccc
     2941 agcacgaccc cgtcgccgaa gcctcggtgg tgcgctgcga tcgccaacgc gaactgcgca
     3001 agctcaagcg catcgacctc ggcgacagcc tggcccaccg catcgctgcg cgctgcatcc
     3061 gccagctcgc caacgacatc gaggacctca acgacaagat cgccgcgctc gacgccgaga
     3121 tcgccgagtt gctcgccgag cacgacaacc cgttcgagga tctcctcggc gcgggcacca
     3181 acctcgcggc caccatcatc gcccaggccg gcgacgtgcg ccgcttccgc gacgcctcgg
     3241 ccttcgcgcg cttctgcggc gccgccccga tcccctgcgg ctcaggccag accagcggcc
     3301 gacaccgcct gcatcgcggc ggcaaccgcc aactcaacgc cgcgctctac cgcatcgcca
     3361 tcgtccaaca acgccaccac cccgccgcca agacctacct cgcccgcaaa atcgccgaag
     3421 gcaagaccgc ccgcgaagcc cgccgcgcgc tcaagcgcca cctcgccaac gtcgtctacc
     3481 gccgcctgct gacctgggcc aacaccaccc cagccctcac cgacttgaca taggagaatc
     3541 gagcgcttca tccgcaccct gctcggcggc tgggcctacg gcgccatcta cgcctcctcg
     3601 gccgagcgca ccgccgccct gaccggctgg ctgtttcgct acaaccacca ccgcaaacac
     3661 agcgtgctcg gccgcaagcc gcccgcagcc cgactcaccg agctggacaa caaccctctt
     3721 gggtcttaca gctagagcgg cgcgagcgag agcgtcgacg tgctcgacg
//
LOCUS       DASVOR010001022         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_68537,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001022 DASVOR010000000
VERSION     DASVOR010001022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68537"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VF516_19740"
     CDS             complement(<1..1085)
                     /locus_tag="VF516_19740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109976.1"
                     /translation="MTDEATPGAKADAKPEARSDGKPEGKGDGKGDGKGDAKPDTKVL
                     AGTDGVSRVTALPPHFPPWAGKLAELYFSGTTSMFVIHGNTFDVVRAGGDGEPRWIGL
                     ADFLAEQLFGRWDLVIHYDLARGLRCAAGSSSRRLQQMVELSNRWLGDLRALPRDPTR
                     ALAAIDLLVQKNVMADPDQRIRTAILIDHAGYVAPSGDRLDLAEQTHLVTLLNWASSP
                     YVKRLNMAFVLIDPRLSSVAERLTGNPHVAAIEVPLPTAEERTAFLAAQLAASPDAGA
                     AFSDYGAAELGKLTAGIGLTDLEVLVRSAREGGRRLDRGYFRELKKRLIERQAQGMLE
                     FVEPRWDLATVVGHDAAKRRLLDDAEL"
     gene            complement(1082..>1860)
                     /locus_tag="VF516_19745"
     CDS             complement(1082..>1860)
                     /locus_tag="VF516_19745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109977.1"
                     /translation="IAPAAANARDRILEVLKSLAEDRRARFLRLRARCVEMTRIANAV
                     RGETGDASGASAELRQPALDRLLWVFLKLLLSDQAIARFLQAADEPQILHSILDLQDR
                     IQKRAASLPEAEREADRILRSLHDSLSTAELRKDNIAKAKGNAEFVAAELERLENKIQ
                     AVTEMAVSHSDPDEMSSRIDAIAEGISQTEQTIRELQSITGMTAQDETPSILGADIAQ
                     PAGSLGPAWPVGSDADDTRRRSDQVWQIAQSRTGRRPPGS"
BASE COUNT          220 a          656 c          698 g          286 t
ORIGIN      
        1 atcagctcgg cgtcgtcgag cagccggcgc ttggcggcgt cgtggccgac cacggtcgcc
       61 aggtcccagc gcggctcgac gaactccagc atgccctggg cctggcgctc gatcagccgc
      121 ttcttgagct cgcggaagta gccgcggtcg agccggcggc cgccctcgcg cgcgctgcgg
      181 accaggacct cgaggtcggt caggccgatg ccggcggtca gcttgccgag ctcggcggcg
      241 ccgtagtcgg agaacgcggc gccggcgtcg ggcgacgccg ccagctgcgc ggcgaggaac
      301 gcggtgcgct cctcggcggt cggcagcggc acctcgatcg cggcgacgtg cgggttgccg
      361 gtcagccgct cggcgacgct ggacagccgc gggtcgatca gcacgaacgc catgttgagc
      421 cgcttgacgt acggcgacga cgcccagttg agcagcgtca ccaggtgggt ctgctcggcg
      481 aggtcgagcc ggtcgccgct cggcgcgacg tagccggcgt ggtcgatcag gatcgcggtg
      541 cggatccgct ggtcgggatc ggccatcacg ttcttctgga ccaggaggtc gatcgcggcg
      601 agcgcccggg tcggatcgcg gggcagggcg cgcaggtcgc cgagccagcg attcgacagc
      661 tcgaccatct gctgcagccg ccggctgctc gacccggccg cgcagcgcag gccgcgcgcg
      721 aggtcgtaat ggatcaccag gtcccagcgg ccgaacagct gctcggcgag gaagtcggcg
      781 aggccgatcc agcgcggctc gccgtcgccg ccggcgcgca cgacgtcgaa cgtgttgccg
      841 tggatcacga acatcgacgt ggtgccggag aagtacagct cggcgagctt gccggcccac
      901 ggcgggaagt gcgggggcag cgcggtgacc cggctgaccc cgtcggtgcc ggcgaggacc
      961 ttggtgtcgg gcttggcgtc gcccttgccg tcgcccttgc cgtcgccctt gccttcgggc
     1021 ttgccgtcgc tcctcgcctc cggcttcgcg tcggccttcg cgcccggcgt tgcctcgtcg
     1081 gtcatgaccc gggcggcctc cgccccgtgc ggctctgggc gatctgccag acctgatcgc
     1141 tccggcgccg ggtgtcatcg gcgtcgctgc ccaccggcca cgccgggccg agcgagccgg
     1201 ccggctgggc gatgtcggcg ccgaggatcg acggggtctc gtcctgtgcc gtcatgccgg
     1261 tgatgctctg gagctcgcgg atcgtctgct cggtctgcga gatgccctcg gcgatcgcgt
     1321 cgatccgcga cgacatctcg tccggatcgc tgtggctgac cgccatctcg gtcaccgcct
     1381 ggatcttgtt ctccagccgc tcgagctcgg cggcgacgaa ctcggcgttg cccttggcct
     1441 tggcgatgtt gtccttgcgc agctccgcgg tcgacaggct gtcgtgcagc gagcgcagga
     1501 tgcgatcggc ctcgcgctcg gcctcgggga gcgacgccgc gcgcttctgg atccggtcct
     1561 ggaggtcgag gatcgagtgc aggatctgcg gctcgtcggc cgcctgcagg aaccgcgcga
     1621 tcgcctggtc cgacagcagg agcttgagga acacccacag cagccggtcg agcgcgggct
     1681 ggcgcagctc ggccgacgcg ccgctggcgt cgccggtctc gccgcgcacc gcgttggcga
     1741 tccgggtcat ctcgacgcag cgcgcgcgca gccgcaggaa ccgggcgcgg cggtcctcgg
     1801 cgagcgactt caggacctcc aggatgcgat cgcgggcgtt ggccgccgcc ggggcgatct
//
LOCUS       DASVOR010001023         3965 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156504,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001023 DASVOR010000000
VERSION     DASVOR010001023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3965
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156504"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1699
                     /locus_tag="VF516_19750"
     CDS             200..1699
                     /locus_tag="VF516_19750"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018478 - malonate-semialdehyde
                     dehydrogenase (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEX8109978.1"
                     /translation="MADKTIPHWIDGKPHDRPADRHGEVFNPATGEVQARVAFASPGV
                     VDEAVEAAARAAQTWRSVSIARRTKILFAFRQLVDQHQHELARLLTLEHGKVTADALG
                     EVNRGLEVIEFACGIADLAKGEYSENVSTEVDSYSIRQPLGVVVGITPFNFPAMVPMW
                     MYPIALACGNAFVLKPSERDPSAANFCAQLLADAGLPPGVFNVVHGDKVAVDRLLEHP
                     RVAAISFVGSTPIARYIYETGTRHGKRVQALGGAKNHMIVLPDADMELAADAAVSAGY
                     GSAGERCMAISVVVAVGSAADRLVPLMKQRIARLKVGDGLEPGTEMGPLINRAHRDRV
                     AGYVDRGIAEGATLVADGRGLTVPGRPHGFFLGPCLFDHVQPSMAIYQDEIFGPVLSM
                     TRAATYDEAIQLVNTNPYANGVALFTNDGGAARKFQHEVEVGMVGINVPIPVPMAYYS
                     FGGWKASLFGDHHIYGRDGVHFYTRTKAITSRWPDPRHRGVDLGFPQNR"
     gene            1998..3116
                     /locus_tag="VF516_19755"
     CDS             1998..3116
                     /locus_tag="VF516_19755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016450659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8109979.1"
                     /translation="MTFAYEPLLESIAATAAQRAASVDRGEFPTETLRALGDAGLLGL
                     ISATEVGGQGLGLAAAAFVVERLARECGSTGRVVCMHFSATAVIEAVGPIEIRRAIAA
                     GQHLTTLALSEAGSRSQFWVPLSTATPEGSDVVLNARKSFATSAGHADSYVWSSKPVS
                     GSELSTLWLVPRTTPGVRVPAAGFDGLGLRGNDSSPIIAEGARIPASARLGNDGGGFA
                     IMMGTVLPWFNVLAAAVSNGLMEAATQRTCSHAAGTQFQHAGTSIADLPTIRAYIARM
                     RIKTDQSKALVADTLAAIAGGRADATLRVLESKAAAGEAAAEVTDIAMRVCGGAAFRK
                     EVGVERIFRDARAATVMGPTTDVLFDFIGKAACGLPLF"
     gene            3119..3922
                     /locus_tag="VF516_19760"
     CDS             3119..3922
                     /locus_tag="VF516_19760"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HEX8109980.1"
                     /translation="MSELVLGAVAYDAKVVPIWDGFKAYFESHGLPFDFVLYSNYERQ
                     VAAHLRGEIDVAWNSPLAWIQTRRAAPDRARAIAMRDTDRDLTSVVLVRATLGPGGSG
                     PGSGAPVQTVADLRAVGVGARDSPQATLIPLLALADAGARVEVVPHDVLVGKHGDHVG
                     GERDAVKALLAGKVDAACVIDGNRLAFAREGLFAPNQLRVLVQTAPYDHCNVTALADT
                     PAIAKLRELLLAMSYDDPVVRPLLDMEGLTRWLPGRTSGYALLEHAVDA"
BASE COUNT          553 a         1482 c         1353 g          577 t
ORIGIN      
        1 tcgaacgacc gcgcgatcac cttgccgttc gtccccggcg gccccgatcc gagcacgaac
       61 gggttgtcga acttcatgcc gttgacggtg atgctgagat cggtcacgtc gcctcctggg
      121 tgcggttgcc gggaaccgga aattgctggg ctagcacggc ctacgagttg gattcaacca
      181 tcatgatagg ctacggcgca tggcggacaa gaccatcccc cactggatcg acggcaagcc
      241 ccacgaccgc ccggcggacc gccacggcga ggtgttcaac cccgcgaccg gcgaggtcca
      301 ggcccgggtc gcgttcgcgt cgcccggcgt cgtcgacgag gcggtcgagg ccgccgcgcg
      361 cgccgcgcag acctggcgct cggtgtcgat cgcccggcgg accaagatcc tgttcgcgtt
      421 ccgccagctg gtcgaccagc accagcacga gctcgcccgc ctgctgaccc tcgagcacgg
      481 caaggtcacc gccgacgcgc tcggcgaggt caaccgcggc ctcgaggtca tcgagttcgc
      541 gtgcggcatc gccgacctcg ccaagggcga gtactccgag aacgtctcga ccgaggtcga
      601 ctcgtactcg atccgccagc cgctcggcgt ggtcgtcggc atcacgccgt tcaacttccc
      661 ggcgatggtg ccgatgtgga tgtatccgat cgcgctggcg tgcggcaacg cgttcgtgct
      721 caagccgagc gagcgcgatc cgtcggccgc caacttctgc gcccagctgc tcgccgacgc
      781 cgggctccca cccggcgtgt tcaacgtcgt ccacggcgac aaggtcgcgg tcgaccgcct
      841 gctcgagcac cccagggtcg ccgcgatctc gttcgtcggc tcgaccccga tcgcccgcta
      901 catctacgag accggcaccc gccacggcaa gcgggtccag gcgctcggcg gcgccaagaa
      961 ccacatgatc gtcctgcccg acgccgacat ggagctcgcg gccgacgccg cggtgtcggc
     1021 cggctacggc tcggccggcg agcgctgcat ggcgatctcg gtggtggtcg cggtcggcag
     1081 cgcggccgac aggctcgttc cgctcatgaa acagcggatc gcccggctca aggtcggcga
     1141 cggcctcgag cccggcaccg agatggggcc gctgatcaac cgcgcccacc gcgaccgggt
     1201 cgccggctac gtcgaccggg gcatcgccga gggcgccacg ctggtcgccg acggccgcgg
     1261 cctcaccgtc cccggccgcc cgcacggctt cttcctcggg ccgtgcctgt tcgatcacgt
     1321 ccagccgagc atggcgatct accaggacga gatcttcggc ccggtgctgt cgatgacccg
     1381 cgccgcgacc tacgacgagg cgatccagct ggtcaacacc aacccgtacg ccaacggcgt
     1441 cgcgctgttc accaacgacg gcggcgccgc ccgcaagttc cagcacgagg tcgaggtcgg
     1501 catggtcggc atcaacgtcc cgatcccggt gccgatggcg tactactcgt tcggcggctg
     1561 gaaggcctcg ctgttcggcg accatcacat ctacggccgc gacggcgtcc acttctacac
     1621 ccggaccaag gcgatcacgt cgcgctggcc ggatccgcgc caccgcggcg tcgacctcgg
     1681 cttcccgcag aaccgctgag gcgagcggct caccgcgggc gcgcgcagcg gatcccgtgg
     1741 atgtcgctcc gcatcgacgg gtcgcgaccg gcctcggcgc aactttccga ccgagcgcga
     1801 atgccgctgg aaatttctgg caagcttccc cttccaccgc gccgcagatc aggtagcgtg
     1861 gcacgtgatg ttccaagttt gagtctccct gaggcccggc gtgtccgggt cacataaccg
     1921 agacggggta cgccggccct ggtgttcgag ctgaccttcg agctccgggc ctccggacgc
     1981 cccgtctgga ggaccccatg accttcgcgt acgagccgct cctcgagtcc atcgcagcca
     2041 ccgccgccca acgcgccgcc agcgtcgatc gcggcgagtt ccccaccgag accctgcgcg
     2101 cgctgggcga cgccggcctg ctcggcctga tcagcgccac cgaggtcggc ggccaggggc
     2161 tggggctcgc ggcggcggcg ttcgtcgtcg agcggctcgc gcgcgagtgc ggctcgaccg
     2221 ggagggtcgt gtgcatgcac ttctccgcga ccgcggtgat cgaggcggtc ggtccgatcg
     2281 agatccgccg cgcgatcgcc gccggccagc acctcaccac gctcgcgctg tccgaggccg
     2341 ggtcgcgcag ccagttctgg gtgccgctgt cgaccgcgac tccggagggc agcgacgtcg
     2401 tgctcaacgc gcgcaagagc ttcgcgacct cggccggcca cgccgacagc tacgtgtggt
     2461 cgagcaagcc ggtctccggc agcgagctgt cgacgctgtg gctggtcccg cgcaccacgc
     2521 ccggcgtgcg ggtgcccgcg gcggggttcg acggcctcgg cctgcgcggc aacgactcgt
     2581 cgccgatcat cgccgagggc gcccggatcc cggcgagcgc tcggctcggc aacgacggcg
     2641 gcgggttcgc gatcatgatg ggcaccgtgc tgccgtggtt caacgtgctc gccgccgcgg
     2701 tgtcgaacgg gctgatggag gcggcgaccc agcggacctg cagccacgcc gccgggacgc
     2761 agttccagca cgccggcacg tcgatcgcgg acctgccgac gatccgcgcc tacatcgcgc
     2821 ggatgcggat caagaccgac cagagcaagg cgctggtcgc cgacaccctc gcggcgatcg
     2881 cgggcgggcg cgccgacgcc acgctgcgcg tgctggagag caaggccgcc gcgggcgagg
     2941 ccgccgcgga ggtcaccgac atcgccatgc gggtgtgcgg cggcgccgcg ttccgcaagg
     3001 aggtcggggt cgagcggatc ttccgcgacg cccgcgccgc cacggtcatg gggcccacca
     3061 ccgacgtgct gttcgacttc atcggcaagg ccgcgtgcgg cctgcccctg ttttaaccat
     3121 gagcgagctc gtcctcggtg ccgtcgccta cgacgcgaag gtcgtcccga tctgggacgg
     3181 cttcaaggcc tacttcgagt cgcacggcct gccgttcgac ttcgtcctgt actccaacta
     3241 cgagcgccag gtcgccgcac acctgcgcgg cgagatcgac gtcgcgtgga actcgccgct
     3301 ggcgtggatc cagacccggc gcgcggctcc ggatcgcgca cgggcgatcg cgatgcgcga
     3361 caccgatcgc gatctgacca gcgtcgtgct cgtccgcgcg actctcggtc ccggcggctc
     3421 cgggcccggc tccggcgcgc cggtgcagac cgtcgcggac ctccgcgcgg tcggggtcgg
     3481 cgcccgggat tctccgcagg cgaccctgat cccgctgctc gcgctcgccg acgccggcgc
     3541 ccgcgtcgag gtcgtccctc acgacgtcct cgtcggcaag cacggcgatc acgtcggcgg
     3601 cgagcgcgac gcggtcaagg cgctgctcgc cggcaaggtc gacgccgcgt gcgtgatcga
     3661 cggcaaccgg ctcgcgttcg cgcgcgaggg cctgttcgcg cccaaccagc tccgcgtgct
     3721 ggtgcagacc gcgccgtacg atcattgcaa cgtcaccgcg ctcgccgaca cgcccgcgat
     3781 cgcgaaactc cgcgagctcc tgctcgcgat gtcgtacgac gatccggtcg tgcgcccgct
     3841 gctcgacatg gagggcctca cccggtggct gcccggccgg acctcgggct acgcgctgct
     3901 ggagcacgcg gtggatgctt gacctcggtg acctcggcct cgacgagggc gcgcacctcc
     3961 tgatc
//
LOCUS       DASVOR010001024         2572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_68571,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001024 DASVOR010000000
VERSION     DASVOR010001024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68571"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF516_19765"
     CDS             complement(<1..180)
                     /locus_tag="VF516_19765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109981.1"
                     /translation="MRLLGIDTATATAGIAIVDEDGRVLAEARHATAGRGADLLVAID
                     QVCRDAGVAPAELDAI"
     gene            complement(188..1855)
                     /locus_tag="VF516_19770"
     CDS             complement(188..1855)
                     /locus_tag="VF516_19770"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8109982.1"
                     /translation="MSILYFLLLVGVLVLIHELGHFAAAKLLDVKVLRFSIGFGRPLI
                     RVKLRETEYQIAAFPCGGYVRILGIEGDDRAGRAAPAAPGSRERSDAGRSFASRPLWQ
                     RLMIVFAGPAANLVLPVIIYFAFFANHSVLPAAVIGDVLDGGAAARAGLEPGDRVLEI
                     DGRPVRYWEELEAAVRSSPGKELHLRVSRNGKVFERYLTPLEETVRYRTGAVTVQGRV
                     GVTHAPFAPLVGVIDSDGPAAHAGLATGDLIISIDGQPVRSWGEVQHHLGKLARRTSL
                     VYFRGLEVPGAPQLQLLAPGFADLVPATQLDAGKRQTYTGLERAEMFVAHVDAGSPAE
                     RAGLRSGDLVTALDDVPVTHWVDLDQRLQAAPDRAFRLTWKRAVAGTTQTMSGELTQV
                     RRRQLDEYGHRVTRLVFGARNDVDRGAGATIAIEGRLGFALSKALERTGETIATIVSG
                     VFQILSGETPSDALGGPLMMYRVASVSGNQGWDSFVLLLALISINLGLINLLPIPMVD
                     GGHLLVFAIEAVSRRPLSLATRERVQNVGLALVLLITALALRNDILR"
     gene            1952..>2572
                     /locus_tag="VF516_19775"
     CDS             1952..>2572
                     /locus_tag="VF516_19775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109983.1"
                     /translation="MASKSGQQEPEKAEVIEELLDALDTALDRVKVLYEQYFLGIQKQ
                     PPAYLHTDAERKIRDLAQIQIRNTALRYRFATLQQKYGSYNSYWRRTLRQIENGTYTR
                     NLYKVGRQAARTGAAVPEEILAAMPKRMREQVVRDREAALALAQLRERQAQDELLTLA
                     DDEVDIDVDDLDAAAFIHEPSEVRRGALTAAGVHRVDESDAEFDVDA"
BASE COUNT          396 a          924 c          934 g          318 t
ORIGIN      
        1 gatcgcgtcc agctccgccg gcgcgacgcc cgcgtcgcgg cacacctggt cgatcgcgac
       61 caggaggtcg gcgccacggc cggcggtcgc gtggcgggcc tcggcgagca cccggccgtc
      121 ctcgtcgacg atcgcgatcc cggcggtcgc ggtcgcggta tcgatgccga gcagcctcat
      181 cgcaggctca tcgcaggatg tcgttgcgca gcgcgagcgc ggtgatgagg aggacgaggg
      241 cgagcccgac gttctggacc cgctcgcggg tcgccagcga cagcggccgg cgcgacaccg
      301 cctcgatcgc gaacaccagc aggtggccgc cgtcgaccat cgggatcggc aacaggttga
      361 tcaggccgag gttgatgctg atcagcgcga gcaggagcac gaagctgtcc cagccctggt
      421 tgcccgacac cgacgcgacc cggtacatca tcagcgggcc gccgagcgcg tcgctcgggg
      481 tctcgccgct caggatctgg aacacgccgg acacgatcgt cgcgatcgtc tcgccggtgc
      541 gctcgagcgc cttggacagc gcgaagccga gccggccctc gatcgcgatc gtcgcgccgg
      601 cgccgcggtc gacgtcgttg cgcgcgccga acaccaggcg ggtcacgcgg tggccgtact
      661 cgtcgagctg gcggcgccgg acctgggtca gctcgcccga catcgtctgc gtcgtgccgg
      721 cgaccgcgcg cttccaggtc agccggaacg cccggtcggg cgcggcctgc agccgctggt
      781 cgaggtcgac ccagtgggtc accgggacgt cgtcgagcgc ggtcaccagg tcgccggacc
      841 ggaggccggc gcgctcggcc ggcgagccgg cgtcgacgtg ggcgacgaac atctcggcgc
      901 gctccagccc ggtgtaggtc tggcgcttcc cggcgtcgag ctgggtggcg gggaccaggt
      961 cggcgaaccc cggcgcgagc agctggagct gcggcgcgcc ggggacctcg aggccgcgga
     1021 agtacaccag gctggtccgg cgcgcgagct tgccgaggtg gtgctggacc tcgccccagc
     1081 tgcgcaccgg ctggccgtcg atgctgatga tcagatcgcc ggtggcgagc ccggcgtgcg
     1141 cggccggccc gtcgctgtcg atcaccccga ccagcggcgc gaacggcgcg tgcgtgaccc
     1201 cgacccggcc ctgcacggtg accgcgccgg tgcggtagcg cacggtctcc tcgagcggcg
     1261 tcaggtagcg ctcgaacacc ttgccgttgc gagagacccg caggtggagc tccttgcccg
     1321 gcgacgaccg caccgcggcc tcgagctcct cccagtagcg caccggccgg ccgtcgatct
     1381 ccagcacccg gtcgccgggc tcgaggcccg cgcgcgcggc ggcgccgccg tcgagcacgt
     1441 cgccgatcac cgccgccggc agcacgctgt ggttggcgaa gaacgcgaag tagatgatca
     1501 ccggcagcac caggttggcc gccggcccgg cgaacacgat catcagccgc tgccacagcg
     1561 ggcggctcgc gaagctgcgc ccggcgtcgc tgcgctcccg ggatcccggc gctgccggcg
     1621 ccgcccgtcc ggcgcggtcg tcgccctcga tgccgaggat ccgcacgtag ccgccgcacg
     1681 ggaacgccgc gatctggtac tcggtctcgc gcagcttgac ccggatcagc ggccggccga
     1741 acccgatcga gaaccgcagc accttgacgt cgagcagctt ggccgccgcg aagtggccga
     1801 gctcgtggat cagcacgagc acgccgacga gcaggaggaa gtacaggatc gacatggggg
     1861 accgggaacg aggctgggaa cgaggctggg aacgaggctg ggaacgaggc tggggccgag
     1921 gctagcgcag aaactgctat cctcccggca cgtggcaagc aagtcgggac agcaggagcc
     1981 cgagaaggcc gaggtcatcg aggagctgct cgatgccctc gacaccgcgc tcgatcgcgt
     2041 gaaggtgctg tacgagcagt actttctcgg catccagaag cagccgcccg cctacctgca
     2101 caccgacgcc gagcgcaaga tccgcgatct cgcccagatc cagatccgca acaccgccct
     2161 gcgctaccgg ttcgccacgc tgcagcagaa gtacggttcg tacaacagct actggcgccg
     2221 cacgttgcgc cagatcgaga acggcaccta cacgcgcaac ctctacaagg tcggccgcca
     2281 ggccgcgagg accggcgcgg cggtgcccga ggagatcctc gccgcgatgc ccaagcggat
     2341 gcgcgagcag gtggtccgcg atcgcgaggc tgcgctggcg ctcgcccagc tgcgcgagcg
     2401 gcaggcgcag gacgagctgc tgacgctcgc cgacgacgag gtcgacatcg acgtcgatga
     2461 cctcgacgcc gccgcgttca tccacgagcc gagcgaggtg cggcgcggcg cgctgaccgc
     2521 cgccggtgtg caccgggtcg acgagtccga cgccgagttc gacgtcgacg cg
//
LOCUS       DASVOR010001025         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448483,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001025 DASVOR010000000
VERSION     DASVOR010001025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448483"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2150)
                     /locus_tag="VF516_19780"
     CDS             complement(<1..>2150)
                     /locus_tag="VF516_19780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109984.1"
                     /translation="LSRRRRPLVRSLPATREVRVAKHLHAAARKLAEHKLGRDHDRVA
                     AVVRDIVERADRHVRVVLRPKIDAALEAVALHPRNLPERVAQKKLVDELLDRAVEVGQ
                     LSLGNLRDAISHNDLKLPDLTRARLRSGDELLRCDDALSRSLDGVYRRGESYLRLLQR
                     ISSVLFGTPLGRVLTLYLILPLLGSYIVFQGAHHMVGAFANHLVGHELEISTPGRIYA
                     GAAFLFLLLHVPVFRRAVVGALRLFGRGLRLVLIDLPRALLRLSVVRWALDTRLARWV
                     VQPAIPAAIAWRFADAVWEPIWRWPFAAAVFAAFALGLNSRLGRRAQEIAADAIVRWS
                     HQLTGRIVPGAIKWLLAMFAELIEIVDRAIYRVDEWLRFKVGQSRLTLVAKGALGLVW
                     AVVAYALRLSINLFIEPEVNPIKHFPVVTVVAKILLPFSEAMMSAISAPVSRLVGSTL
                     GVSFAAFAVFILPGFAGFLVWEFKENWRLYRNTRPETLHEVSFGHHGETMVGFLKPGF
                     HSGTIPKRFTKLRRAAWKSDERGVARHREELHHIEDTIRTFVDRELVSMLDAAPAFRI
                     PDVAVAHVAIGSNRVHIDLACPGVGPDLARIAIEQQSGWTVASLPERGWIDRLTDGQR
                     QILEIALAGFYKRSGIDLVREQLEHALADDADPPPYDISDDGLVVWPAHGYQTEAVYN
                     LHARQPVAAMRGAPWSGDPPILAGRSAVFRREPL"
BASE COUNT          337 a          676 c          879 g          258 t
ORIGIN      
        1 cagcggctcg cggcggaaca ccgcgctgcg gccggcgagg atcggcggat cgccggacca
       61 cggagcgccg cgcatcgcgg cgaccggctg gcgcgcgtgg aggttgtaga ccgcctcggt
      121 ctggtagccg tgggcgggcc acacgacgag gccgtcgtcg gagatgtcgt aggggggcgg
      181 gtcggcgtcg tcggcgagcg cgtgctcgag ctgctcgcgg accaggtcga tgcccgagcg
      241 cttgtagaag ccggcgaggg cgatctcgag gatctggcgc tggccgtcgg tgaggcggtc
      301 gatccagccg cgctcgggga ggctggcgac ggtccagccc gattgctgct cgatggcgat
      361 gcgggcgagg tcggggccga cgccggggca cgcgagatcg atgtggacgc ggttggagcc
      421 gatcgcgacg tgggcgaccg cgacgtcggg gatccggaac gcgggggcgg cgtcgagcat
      481 cgagaccagc tcgcggtcga cgaacgtgcg gatcgtgtcc tcgatgtggt gcagctcctc
      541 gcggtggcgg gcgacgccgc gctcgtcgga cttccaggcg gcgcgccgca gcttggtgaa
      601 ccgcttgggg atggtgccgg agtggaagcc gggcttgagg aagccgacca tggtctcgcc
      661 gtggtggccg aacgagacct cgtgcagggt ctcggggcgg gtgttgcggt acagccgcca
      721 gttctccttg aactcccaga ccaggaagcc ggcgaagccg ggcaggatga acaccgcgaa
      781 cgcggcgaac gacacgccga gggtggagcc gaccaggcgg ctgacggggg cgctgatcgc
      841 gctcatcatc gcctcggaga acggcaggag gatcttggcg accacggtga cgaccggaaa
      901 gtgcttgatc gggttgacct cgggctcgat gaacaggttg atcgagagcc gcagggcgta
      961 ggcgacgacg gcccagacca ggccgagcgc gcccttggcg accagggtca ggcgcgactg
     1021 gccgaccttg aaccgcagcc actcgtcgac gcggtagatc gcgcggtcga cgatctcgat
     1081 cagctcggcg aacatcgcga gcagccactt gatggcgccg gggacgatgc ggccggtgag
     1141 ctggtggctc cagcgcacga tggcgtcggc ggcgatctcc tgggcgcggc ggccgaggcg
     1201 cgagttgagg ccgagggcga acgcggcgaa cacggcggcg gcgaacggcc agcgccagat
     1261 cggctcccag acggcgtcgg cgaaccgcca ggcgatcgcg gccgggatcg cgggctggac
     1321 gacccagcgc gcgaggcggg tgtcgagcgc ccagcgcacc accgagaggc ggagcagggc
     1381 gcgcggcagg tcgatcagca cgagccgcag gccgcgcccg aacaggcgga gcgcaccgac
     1441 gacggcgcgg cgaaacaccg ggacgtggag cagcaggaac aggaaggcgg cgccggcgta
     1501 gatccgcccc ggggtcgaga tctcgagctc gtggccgacc aggtgattgg cgaacgcgcc
     1561 gaccatgtgg tgcgcgccct ggaacacgat gtacgagccg agcagcggca ggatcaggta
     1621 cagcgtgagc acgcggccga gcggggtgcc gaacaggacc gacgagatcc gctggagcag
     1681 ccgcaggtag ctctcgccgc gccggtacac gccgtcgagc gagcgcgaca gcgcgtcgtc
     1741 gcagcgcaag agctcgtcgc ccgagcgcag ccgcgcccgg gtgaggtcgg gcagcttgag
     1801 gtcgttgtgc gagatcgcgt cgcgcaggtt gccgagcgag agctggccga cctcgaccgc
     1861 gcggtcgagc agctcgtcga ccagcttctt ctgggcgacc cgctcgggca ggttgcgggg
     1921 gtggagcgcg accgcctcga gcgcggcgtc gatcttggga cgcagcacga cgcggacgtg
     1981 gcgatcggcg cgctcgacga tgtcgcggac gacggcggcg acccggtcgt gatcgcgccc
     2041 gagcttgtgc tcggcgagct tgcgggcggc ggcgtggagg tgcttggcga cccggacctc
     2101 gcgggtcgcc ggcagcgagc ggaccagcgg ccggcgccgg cgcgagagca
//
LOCUS       DASVOR010001026         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156512,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001026 DASVOR010000000
VERSION     DASVOR010001026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156512"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(161..496)
                     /locus_tag="VF516_19785"
     CDS             complement(161..496)
                     /locus_tag="VF516_19785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109985.1"
                     /translation="MEPTPTPSEPTGLDFAGTCDLAIWCKLLHDKGWSGPRIARAIAK
                     SEGYVNNLIRVVERASPSIMLRWRAEQNGLVDHVCATDWLVAVCLLSHDRQDEELQRR
                     IAARPGYRV"
     gene            551..964
                     /locus_tag="VF516_19790"
     CDS             551..964
                     /locus_tag="VF516_19790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109986.1"
                     /translation="MTSAGYSGTPLPQKLGIKDGYRVAVVGAPPGFALGALPAGTHLQ
                     RNLAGRTPLDVLVVFVTRRADLVTRIATCRPRMQPAAGLWIAWPKRASGMATDITEDT
                     VREVALPTGLVDNKVCAIDDTWSGLRLVIRKELRT"
     gene            complement(1015..>1617)
                     /locus_tag="VF516_19795"
     CDS             complement(1015..>1617)
                     /locus_tag="VF516_19795"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEX8109987.1"
                     /translation="AAADPPRRFAGQTYWARPVPGFGDPTARLLVVGLAPAAHGGNRT
                     GRVFTGDRSGDWLYAALHRAGFANQSASVARGDGLALAGAYVSCVVRCAPPGNKPAPR
                     ERDRCVGYLARELQLLTRVRAIVALGGFAWDGVQVAAAELGHPIPRPRPKFGHGAEVR
                     IGPWLVVGSYHVSQQNTFTGRLTEPMLDQVFARARAALAA"
BASE COUNT          238 a          577 c          593 g          209 t
ORIGIN      
        1 tcgcgctcgc caagtcgcgc gacgcgcggt tcgcgtccgg cgccgagctc gcgatggcgt
       61 tcgccgccgc cgaccagggc gcgctgcccg acctgctggt ccgccgcgcc cgccagctcg
      121 cccagcagct ggcgtggacc gagcccgatc ggcccgaagg tcagacgcga tagcccggcc
      181 gcgcggcgat ccggcgctgc agctcctcgt cctggcggtc gtgcgacagc aggcacaccg
      241 cgaccagcca gtcggtcgcg cacacgtggt cgaccaggcc gttctgctcg gcgcgccacc
      301 gcagcatgat cgacggcgag gcccgctcga cgacgcggat caggttgttg acgtagcctt
      361 cgctcttggc gatcgcgcgc gcgatccgcg ggccggacca gcccttgtcg tgaagcagct
      421 tgcaccagat cgccagatcg caggtgcctg cgaagtcgag cccggtgggt tcagaggggg
      481 tgggggtggg ttccatgggg atcatcgacg gctctcgcta cacagacgcc cgcggtggca
      541 cgatcattcc atgacaagcg cgggctattc ggggacacca ttgccacaga agctcggaat
      601 caaagatgga tatcgcgtcg ccgtggtcgg cgcgccgccc gggttcgcgc tcggcgcgtt
      661 gccggccggc acgcacctgc agcgcaacct cgccggcagg acgccgctcg acgtgctcgt
      721 cgtgttcgtg acccggcgcg ccgatctggt gacgcggatc gcgacctgcc ggccgcggat
      781 gcaacccgcg gccggactgt ggatcgcgtg gccgaagcgc gcctcgggca tggcaaccga
      841 tatcacggag gacaccgttc gcgaggtcgc gttgccgacc ggcctggtcg acaacaaggt
      901 gtgcgcgatc gatgacacct ggtcggggtt gcggctggtg atccgcaagg aactccggac
      961 gtagcaccgc taccgacaac gcacgggcgg cggcctggat ctacgcggcc tgatctacgc
     1021 ggccaacgcc gcgcgggccc gggcaaatac ctggtcgagc atgggctcgg tgagccggcc
     1081 ggtgaacgtg ttctgctggc tgacgtggta cgagccgacc acgagccacg gcccgatgcg
     1141 gacctcggcg ccgtggccga acttcgggcg cggccgcggg atcggatggc cgagctcggc
     1201 cgcggcgacc tgcacgccgt cccacgcgaa cccgccgagc gcgacgatcg cgcggacgcg
     1261 ggtcaacagc tggagctcgc gggcgaggta gcccacgcag cggtcgcgct cgcgcggcgc
     1321 cggcttgttg ccgggaggcg cacagcgcac cacacaggac acgtaggcac ccgccagcgc
     1381 caggccgtca ccgcgcgcca ccgatgcgga ctggttggca aaccctgcgc gatggagcgc
     1441 ggcgtacagc cagtctcccg accgatcgcc ggtgaacacg cggccggtgc ggttgccgcc
     1501 gtgggcggcg ggcgcgaggc cgaccacgag cagccgggcc gtcggatcgc cgaacccggg
     1561 caccggccgc gcccagtagg tctggccggc gaaccgccgg ggcggatccg cggcggc
//
LOCUS       DASVOR010001027         3561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_506969,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001027 DASVOR010000000
VERSION     DASVOR010001027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_506969"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /locus_tag="VF516_19800"
     CDS             <1..1891
                     /locus_tag="VF516_19800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8109988.1"
                     /translation="IKRVEMITGPGGVLWGSNSLLGILNVITKDAEDVDGVEVGGQVG
                     DGGGDRLMARAYAMAGAANLLGGKLKAFVHASVQTWQGPAFEMPLLLFHNALPQPNSP
                     NVYGPLTTSDQAQSMITTLDGKLTYDKLQLRVYVPFGHEYNPMGLSGEPVRQTLPEDA
                     MCPVGSTAAGCTDPNRLSRLNREDVYDRYAVAEYRTRFAHDKAGIAVRGYVQQFVRGF
                     EPLQILSPSATIPGGLSFKADLTSYRTGGAFDGDVELTSQIRVLYGAEAFHEWKPDRS
                     SGSLQGTGTPSTLISPSPSLVPLPCPRDGTGQPVAGCPLTFAFPADRTVLGAYVDPQY
                     RPNKQLIFDLGGRVQVAPASLGSLHYDLNTEVAATVVWNFIPNWHLKVNYAQGFRPPV
                     FNNTTSNGEALQIGGNPNLTVERSEATQAEINARIFKSERQIRELSFRIDGSYTRLND
                     LIQVTSGNYSNSGDRGLASLEFLGKLYLQGGHRIEFGYTYLQGDTSDKGRLRALPENW
                     FSLAGVWSLIGNQLTATSTLKITGAAEDPNRLVEYRGAAAASPVTVLATDLVMDRLPP
                     SADLSLGLSYAPTPKLAVRVMAYNALFGHTYQPDVYFDYEPHLEYLPNPYEGFRAYLS
                     ATYQY"
     gene            1963..2946
                     /locus_tag="VF516_19805"
     CDS             1963..2946
                     /locus_tag="VF516_19805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109989.1"
                     /translation="MSKRRVLAISSDLDQLRRIVVTLERAGAEVDAVRSSEAIAAEVI
                     PHRYVFFSATNGEVGALDALVPKLRERAHVTVVTPRAELSELTSYLRDDRINHVVVGD
                     ELESGVFVTAQKLLTGDIFGIEKYLPAGTPVHYVRLHDFIGRGRAIDTVLHYAEDARM
                     RRQVRTQIAAVCEELLMNALYDAPVDAAGRPIFADVDPHDRKGTHSPRPVSIRYAATD
                     QGFAVAVRDRFGRLAKNTVLSYIEKCIRSPVQIDRKTYGAGLGLYLVANAAASYVVNV
                     AYGIATEVVCTFDRGAKSPLRLVGMFVHPGGAEMLKRGPTPETAAGLRRSG"
     gene            2939..>3561
                     /locus_tag="VF516_19810"
     CDS             2939..>3561
                     /locus_tag="VF516_19810"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109990.1"
                     /translation="MAERQFGPYKLVRQIAVGGMAEIHLAKTAGIAGFEKYVALKMIH
                     PNFAEDEQFIQMLVDEAKIAVQLTHGNIAQTFDLGRVGETYYITMEYVDGADLYKILR
                     RASEQDLEMPLDVCAFLGKEVSSALDHAHRKRDHAGKSLGIVHRDVSPQNVLVSYSGE
                     VKLVDFGIAKATMKVRQTAVGVIKGKYYYMSPEQAWGDPIDHRSDIFS"
BASE COUNT          594 a         1250 c         1173 g          544 t
ORIGIN      
        1 gatcaagcgg gtcgagatga tcaccggccc gggcggcgtg ctgtggggct cgaactcgct
       61 gctcggcatc ctcaacgtga tcaccaagga cgccgaggac gtcgacggcg tcgaggtcgg
      121 cggccaggtc ggcgacggcg gcggcgatcg cctgatggcg cgcgcctacg cgatggccgg
      181 cgcggccaac ctgctcggtg gcaagctcaa ggcgttcgtc cacgccagcg tgcagacctg
      241 gcagggtccg gcgttcgaga tgccgctgct cttgttccac aacgcgctgc cccagccgaa
      301 ctcgcccaac gtgtacggcc cgctgaccac gagcgatcag gcgcagtcga tgatcaccac
      361 gctcgacggc aagctgacct acgacaagct ccagctccgg gtctacgtcc cgttcggcca
      421 cgagtacaac ccgatggggc tgtcgggcga gccggtgcgc cagaccctgc ccgaggacgc
      481 gatgtgcccg gtcggatcga ccgcggcggg ctgcaccgac cccaaccggc tcagccggct
      541 caaccgcgag gacgtctacg atcgctatgc ggtcgccgag taccgcacgc ggttcgcgca
      601 cgacaaggcc gggatcgcgg tgcgcggcta cgtgcagcag ttcgtccgcg ggttcgagcc
      661 gctgcagatc ctgtcgccga gcgccaccat ccccggcggc ctgtcgttca aggccgacct
      721 caccagctac cgcaccggcg gggcgttcga cggcgacgtc gagctgacgt cccagatccg
      781 cgtgctgtac ggcgccgagg cgttccacga gtggaagccc gaccggagct ccggttcgct
      841 gcagggcacc ggcacgccgt ccacgctgat ctcaccgtcg cccagcctgg ttccgctgcc
      901 gtgcccgcgc gacgggacgg gccagccggt ggcaggctgc ccgctgacgt tcgcgttccc
      961 ggccgaccgc acggtgctcg gcgcctacgt cgatccgcag taccggccga acaagcagct
     1021 gatcttcgac ctcggcggcc gcgtccaggt ggcgccggcg tcgctgggca gcctgcatta
     1081 cgacctcaac accgaggtcg ccgcgaccgt ggtgtggaac ttcatcccca actggcacct
     1141 caaggtcaac tatgcccagg gcttccggcc gccggtgttc aacaacacca cgtccaatgg
     1201 tgaagctcta cagatcggcg gtaacccgaa cctgacggtc gagcgctcgg aggcgaccca
     1261 ggccgagatc aacgcccgta tcttcaagag cgagcggcag atccgcgagc tgtcgttccg
     1321 gatcgacggc agctacaccc ggctcaacga cttgatccag gtcacgtcgg ggaactactc
     1381 caactccggc gaccgcggcc tcgcctcgct ggagttcctc ggcaagctct acctccaggg
     1441 cggccaccgg atcgagttcg gttacacgta cttacagggc gacacgagcg acaagggccg
     1501 gctgcgcgcg ctgccggaga actggttctc gctggcggga gtgtggtcgc tgatcggcaa
     1561 ccagctgacc gcgacctcga cgctcaagat caccggcgcc gcggaggacc ccaaccgcct
     1621 ggtcgagtac cgaggtgcgg cggctgcgtc gccagtcacc gtcctcgcga cagatcttgt
     1681 gatggaccgg ctgccgccga gcgccgacct gtcgctcggc ctgtcgtacg cgccgacacc
     1741 caagctggcg gtccgggtca tggcctacaa cgcattgttt ggccacacct accagcccga
     1801 cgtctacttc gactacgagc cgcacctgga ataccttccc aatccctacg agggcttccg
     1861 ggcgtacctg tcggcgactt atcagtacta gccgccgcgt caggcgcgcc cggccctgtt
     1921 atcatcgtac ggcgagggcg gtgcgcccga gcacggggaa tcttgtcaaa acgtcgtgtg
     1981 ctcgcgatct ccagcgatct cgaccagctc cgccgcatcg tcgtcacgct cgaacgcgcc
     2041 ggggccgagg tcgacgccgt gcgcagctcc gaggcgatcg ccgccgaggt gatcccgcac
     2101 cgctatgtgt tcttttccgc gaccaacggc gaggtcggtg cactcgatgc cctggtgccc
     2161 aagctgcgcg agcgcgccca cgtcacggtg gtcacgccgc gcgccgagct gtccgagctg
     2221 accagctacc tgcgcgacga ccggatcaac cacgtggtgg tcggtgacga gctcgagagc
     2281 ggcgtgttcg tcaccgccca gaagctgttg accggcgaca tcttcgggat cgagaagtac
     2341 ctgccggcgg ggaccccggt gcactacgtc cggctgcacg acttcatcgg ccgcggccgg
     2401 gcgatcgaca ccgtgctgca ctacgccgag gacgcccgga tgcgccggca ggtccggacc
     2461 cagatcgccg cggtgtgcga agagctgctg atgaacgcgc tgtacgacgc cccggtcgat
     2521 gccgccggcc ggccgatctt cgccgacgtc gacccgcacg accgcaaggg cacgcactcg
     2581 ccgcgcccgg tgtcgatccg ctacgcggcg accgaccagg ggttcgcggt cgcggtgcgc
     2641 gaccggttcg gccggctcgc gaagaacacg gtcctgtcgt acatcgagaa gtgcatccgg
     2701 tcgccggtcc agatcgatcg caagacctac ggcgcgggcc tcgggctcta cctggtcgcc
     2761 aacgcggcgg cgagctacgt ggtcaatgtc gcctacggga tcgcgaccga ggtcgtgtgc
     2821 acgttcgacc gcggggcgaa gtcgccgctc cggctggtcg ggatgttcgt ccacccgggg
     2881 ggcgccgaga tgctgaagcg gggaccgacg ccggaaaccg cagcggggtt gaggcgcagt
     2941 ggctgagcgt cagttcggac cgtacaagct cgtccgccag atcgcagtcg gcggcatggc
     3001 cgagatccac ctcgcgaaga ccgccgggat cgccgggttc gagaagtacg tcgcgctcaa
     3061 gatgatccac cccaacttcg ccgaggacga gcagttcatc cagatgctcg tcgacgaggc
     3121 caagatcgcg gtccagctga cccacggcaa catcgcgcag accttcgacc tcggccgggt
     3181 cggcgagacc tactacatca cgatggagta cgtcgacggc gccgatctct acaagatcct
     3241 gcggcgcgcc tcggagcagg acctcgagat gccgctcgac gtgtgcgcgt tcctcggcaa
     3301 ggaggtgtcg tcggcgctcg atcacgcgca ccgaaagcgc gatcacgccg gcaagtcgct
     3361 cggcatcgtc caccgcgatg tctcgccgca gaacgtgctg gtgtcgtact ccggcgaggt
     3421 caagctggtc gacttcggca tcgccaaggc gacgatgaag gtccgccaga ccgcggtcgg
     3481 cgtgatcaag ggcaagtatt actacatgag ccccgagcag gcgtggggcg atccgatcga
     3541 ccaccgctcg gacatcttct c
//
LOCUS       DASVOR010001028         4905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_418853,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001028 DASVOR010000000
VERSION     DASVOR010001028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418853"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..706)
                     /locus_tag="VF516_19815"
     CDS             complement(<1..706)
                     /locus_tag="VF516_19815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109991.1"
                     /translation="MNKLKFLVIAALVAAPASAFAQPVLQARCTTDSLDPTQAIARLD
                     WARRCGLLNNTGTVPNTGNPNNFFPSTKAFDQATFLGANDYRENNSLKAFSGSTNDLD
                     INYSMGWSLNNVPLYTVARETSGLTLNFWKWSSTTQRARPLYPAFDTTTNGTGTQMFP
                     SPTLTGCGLFFDKAGTQPVPATTNFFVVAYCESSCYTPDQSLRFSTGDVNILDAFNAL
                     RDDVVTLSSDATLNNPK"
     gene            complement(809..2314)
                     /locus_tag="VF516_19820"
     CDS             complement(809..2314)
                     /locus_tag="VF516_19820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109992.1"
                     /translation="MKLRSAFLLLTSLLAGSACATSDEPDTSEAPQPLTLDDRIAACT
                     NDPRVKIGKLSVDTCVGADLFFRETFNGNGRSCGTCHRVDRNLTIDPAFMATLGASDP
                     LFVAEFNPTLSQLEVPTVMRKFGLILENVDGFNPDPRINFVMRSVPHTLSMSTSITNP
                     TGTPNPPANRTGWSGDGAPGNGALRDFQTGAITQHYTKSLSRTVGTDFRLATSGELDR
                     IDKFMRQTGRLNELNLNAVVLTDAGAEAGRGLFLTKGCNGCHGNAGANASFGGGGNRN
                     FNTGVETARNAELAGFPHDGGFGTAPANGAFGDGTFNVPPLIEAADTGPFFHTAVTIT
                     GTENHDTVFAQTIEEAVAFYDTAEFNASPAGLAVPIELAGDEIDNLGCFLRVLNAGFN
                     AAQAAKRFDAAVAVTNTFGDGARSTETQLIRLACVDLQDAFNELSAVSCNLPQAAGFN
                     QAISQCRSALVAQTPASRASSATQAMQTAAAAAAGLGTNLNYQIGEGTVTF"
     gene            3264..3458
                     /locus_tag="VF516_19825"
     CDS             3264..3458
                     /locus_tag="VF516_19825"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8109993.1"
                     /translation="MRKSLVDEELEAMFAAAAQVIDGRHATSPQEQMYIEPQAMMAEW
                     RDGRCHVAGSMQCRPTCTRR"
     gene            3759..>4905
                     /locus_tag="VF516_19830"
     CDS             3759..>4905
                     /locus_tag="VF516_19830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEX8109994.1"
                     /translation="MNSKLTCSILAATITLVPTAVLAEISQESRCQSDSLDTTQVLER
                     LDWARQCGLLTNTGGPASWVNSTHALDAVSVPPSQIVWAKEYVENNPNHAYSGNLEAY
                     NVNYYYSWALYESTPMYTMIREASGFTSTYWKWSHTVQRARPMYPTFETSPVAGAGTQ
                     LFPGLYNTVVLPPPDPCGGFSRAVDSSLDSNINMMPICPLVADTTTSLTTQVEIDCGF
                     YTDPARTIPYTGSNVYVSAYCESGCYTPDQSLRFSDGDVNILDAMKAQRDDLVTLTPD
                     ATLDNPVTQTSKVYSYITDIRDAEHIIYQITTASGGLLSVTNEHPLITQEGRLVKAEK
                     LAAGDQLLKADGTPDRITGIMKTKYFGKVYNIKPVSREPVANILIAQG"
BASE COUNT          915 a         1498 c         1547 g          945 t
ORIGIN      
        1 tcttgggatt gttgagggtc gcgtcagacg acagcgtgac gacatcgtcc cgcagcgcgt
       61 tgaaggcatc gaggatgttc acgtcgccgg tgctgaaccg caggctctgg tcgggcgtgt
      121 agcagctcga ctcgcagtaa gcgaccacga agaagttggt cgtggccgga accggctggg
      181 tgccggcctt gtcgaagaag agcccacagc cggtcaaagt ggggctgggg aacatctggg
      241 tcccggtacc gttcgtggtg gtgtcgaagg ccgggtacag gggacgtgcg cgctgcgtcg
      301 tcgaggacca cttccagaag ttcagggtga ggccggacgt ctcccgggct accgtgtaca
      361 gcgggacatt gttgagcgac cagcccatcg agtagttgat gtcgaggtcg ttggtgctgc
      421 cggagaacgc cttgagcgag ttgttctcgc ggtagtcgtt ggcgccgaga aaggtcgctt
      481 gatcaaatgc cttcgtgctc gggaaaaagt tgttcggatt tccggtgttg ggaaccgtac
      541 ctgtgttgtt gaggagtccg cacctgcggg cccagtcgag gcgtgcgatc gcctgagtcg
      601 ggtcgaggct gtcggtggtg cagcgcgctt gcaggacggg ctgcgcgaac gccgaagcgg
      661 gagctgcgac cagcgcagcg atgactaaaa acttgagctt gttcattggt ctcctggttg
      721 gcaagtgaag aacgatagcg gaacatggaa ctcgaagaga gagctaagaa tcgaggaaat
      781 tcgagagagc agcgtgagga gcgggcgctc agaacgtcac ggtgccttcg ccgatctggt
      841 agttcaggtt cgtgccgagg ccggccgccg cggccgctgc ggtctgcatc gcctgcgtgg
      901 ccgaggacgc gcgactggcg ggcgtttggg cgacgagcgc gctgcggcac tggctgattg
      961 cctggttgaa accagccgcc tggggcaggt tgcagctcac cgcgctgagc tcgttgaagg
     1021 cgtcctgcag gtcgacgcat gcgagccgga tcagctgggt ctccgtgctg cgcgctccgt
     1081 caccgaaggt gttggtcacc gccaccgcgg catcgaaccg cttggccgcc tgcgccgcgt
     1141 tgaatccggc gttgaggacg cgcaggaaac agccgaggtt gtcgatctca tcgccggcga
     1201 gctcgatcgg cacggccagt ccggccggcg aggcgttgaa ctccgccgtg tcgtagaacg
     1261 cgaccgcctc ttcgatcgtc tgggcgaaga cggtgtcgtg gttctcggtg ccggtgatcg
     1321 tgaccgcggt atggaagaac gggccggtgt ccgcggcttc gatcagcggt ggtacgttga
     1381 acgtgccatc gccgaacgcg ccgttggccg gggcggtgcc gaatccgccg tcgtgcggga
     1441 accctgcgag ctccgcgttg cgggccgtct cgacgccggt gttgaagttg cgattgccgc
     1501 cgccaccgaa gctggcgttg gcgccggcgt tgccgtgaca cccgttgcat cccttggtga
     1561 gaaacagccc gcgtccggcc tcggcacccg cgtccgtcaa cacgaccgcg ttgaggttga
     1621 gctcgttgag ccggccggtc tggcgcatga acttatcgat ccggtcgagc tcgccgctcg
     1681 tggcgagccg gaagtccgtg cccaccgtgc gcgagagcga cttggtgtag tgctgcgtga
     1741 tcgcgccggt ctggaagtct cgcagcgcac cgttacccgg cgcaccatct ccggaccacc
     1801 cggtccggtt ggccggggga ttcggcgtgc cggtcggatt cgtgatgctg gtggacatcg
     1861 acagcgtgtg cggtacggaa cgcatcacga agttgatacg tggatcgggg ttgaagccat
     1921 cgacattttc gaggatcaag ccgaacttcc gcatcaccgt ggggacctcg agctgcgaga
     1981 gcgtcgggtt gaattcggcc acgaacaacg gatcgctggc cccgagcgtc gccatgaacg
     2041 ccgggtctat cgtgaggttg cgatcgacgc ggtggcacgt cccacatgac cgtccgttgc
     2101 cgttgaacgt ctcgcgaaag aacaggtccg cgccgacgca ggtatcgacg ctgagcttcc
     2161 cgatcttgac gcgcggatca ttcgtgcatg cagcgatccg gtcatccagc gttagaggct
     2221 gaggggcttc ggatgtgtcg ggttcgtcgc tggtcgcgca cgccgatccg gcgagcaagc
     2281 ttgttagtaa aagaaacgca ctgcgtaact tcatgaagac atccaccttt tgtgatttga
     2341 atacagagca ccaactggca tgcagaatgc ccactggcac ctctgcgcca tgatcccgtt
     2401 gattcattaa cctggtgtgg cgtagtgccg caagcattaa atcagctcag ctaagaaatt
     2461 tcttcacccc catagacgca gctatttgct gcatgagatg aacatcgcag tcagcgcttt
     2521 gctgcagatc tgttactggc aacgattctg gtagcttggc gcgagctgat tgacactcag
     2581 tgatcggcat cagcacaggt caatgtgcga gctcagcggg gctagcgtta ggagttcttg
     2641 actatggacc aagcacacga gatcccgaga gggacgagca ggtggttttc gcttttgcgc
     2701 ccaggcgaag tccggggggt ggcgtcagcg ccctggtccg aggaacgagc gtaagactgc
     2761 ttaccgttta tagcgcaagg gagacgattg cggtttctac gacaccaatc ggcaacctgc
     2821 tgcagtacac gcccgaaacg gctgcgcaag gttcctgcgc tgcacaaccg tatgcttctc
     2881 tgtgcatgtt tcgaacagtg cgacgccgag cacttcgaag ctaccagtgc cgtcgtcgtt
     2941 gccggttggc cggcggatgt cgatgcgctt ggatttcgcc ggatcgaggg tgagactgtc
     3001 cttcgcgaat tgcttcgcga gcagcttcct cgccgtcgcg tgtccgcttc ccgcgcacgg
     3061 actcgacgag gcgatgatcg tggtcggcgc gcagcatggc gacgtgatcg gacctgggtc
     3121 ccccgacgac gaagaccgtt tcgcgtcgct gagccacgcc gagcgctcgc tctacttctt
     3181 cttcggatcg caacggcgtc agcttctacg cctacgaccg gatctgcgac gccctcgagc
     3241 tcgcgccgga ggcgtatctc ctcgtgcgca agagcctcgt cgacgaggag ctcgaggcga
     3301 tgttcgcggc ggcggcgcag gtgatcgacg ggcgccacgc gacctcgccg caggagcaga
     3361 tgtacatcga gccgcaggcg atgatggccg agtggcgcga cggccgctgc cacgtcgcgg
     3421 gctcgatgca gtgccgtcct acgtgcacaa ggcgatgaag gcgcgtcaag cagtggcgcg
     3481 cggagccgtg ctccttcgag agttactcga atgagggccg gcttcctcaa ggtaactggc
     3541 cattcttgcg gccctggcag gtcatccatc acctgcgtga ccatcgtccc atccctggcc
     3601 ccggaagaaa attgtactat cgaagacagc ccaatgagcg tatgatgaat gttcttgacg
     3661 gatgagaacg ctcagatggc agagaccgct ctgccgtctc gagacgttca tttggccatg
     3721 tccgtacgaa tgcccattcc agtgaaggag taccctgaat gaactcaaaa ctcacgtgtt
     3781 cgatcctcgc cgcaaccatc acgcttgttc caacagctgt cctcgccgag atctcgcagg
     3841 agtcgcgctg ccaatcggac agcctcgaca cgacccaggt gctcgagcgc ctcgattggg
     3901 ctcgtcagtg cggtctcctc accaacaccg gtggcccagc cagctgggtc aactccaccc
     3961 acgcccttga tgcggtgtcg gtgcccccca gtcaaattgt ttgggccaag gagtacgtgg
     4021 agaacaatcc taaccatgcc tatagcggca acctcgaagc ttataatgtc aactactatt
     4081 acagctgggc cctctacgag tccacgccga tgtacacgat gattcgtgag gcgagtggct
     4141 tcaccagcac gtattggaag tggtcgcaca ccgtccagcg cgcgcgcccc atgtatccga
     4201 ccttcgagac cagcccggtg gccggcgctg gaacccagct cttccccggc ctctacaaca
     4261 ccgtggtctt gcccccgcct gacccgtgcg gggggttctc gagagccgtg gactcgagcc
     4321 tggactcaaa cataaacatg atgccaatat gtccgctcgt ggcggacacc acgacctccc
     4381 tcacgaccca ggtagagatc gactgcggtt tctacacgga ccctgcacgc accatcccgt
     4441 acaccgggag caacgtctac gtgtccgcct actgcgagtc tggctgctac acgcccgacc
     4501 agagccttcg cttcagcgac ggtgacgtca atatcctcga cgccatgaag gcccagcgcg
     4561 acgacctcgt cacccttacc ccagacgcca ccctcgacaa tcccgtcacc cagacctcca
     4621 aggtctatag ctacatcacc gacatccgcg acgccgagca catcatctac cagatcacca
     4681 ccgcctctgg tggtctgctc agcgtcacca acgagcatcc cctgatcacc caggagggcc
     4741 gtctggtcaa ggccgagaag ctcgccgcgg gcgatcagct gctcaaggcc gatggcaccc
     4801 cggatcgcat caccgggatc atgaaaacca agtacttcgg caaggtctac aatatcaagc
     4861 ccgtgtcccg tgagcccgtc gccaatatcc tcatcgcgca gggct
//
LOCUS       DASVOR010001029         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_215088,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001029 DASVOR010000000
VERSION     DASVOR010001029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215088"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VF516_19835"
     CDS             <1..288
                     /locus_tag="VF516_19835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109995.1"
                     /translation="VSELMLSLHRLTDDPGYADFARVLADDIVRRATRRSDGGLEWVH
                     AENRVQPYWRQSYTGYMQGAAGIGSLFIRLAAHDRRLGWKVRLPDNPLPIV"
     gene            complement(327..1151)
                     /gene="argB"
                     /locus_tag="VF516_19840"
     CDS             complement(327..1151)
                     /gene="argB"
                     /locus_tag="VF516_19840"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEX8109996.1"
                     /translation="MTRPTIIKIGGEVIGSGEAGVLAGDVKALVDGGARIAIVHGGGP
                     QATDLQKRLGIPTVQVAGRRVTDPATLDVMKMVLAGKLNVDLCATLLAAGLAPVGLHG
                     ASGHLIRAVRRPPKAYPSAGPDPVDLGLVGDVTGFHLPLLDTLWSAGYLPVIACLGAD
                     AAGGVYNINADLVGNQLAAALRAERLFLVTSTPGVLRDVNDPGSRLTRLSCADARRAI
                     ADGVVTGGMIAKLEEAMAVIDQGVGAIHILGKLAAGDLVRAVREPGSVGTTLVADA"
     gene            complement(1148..>1999)
                     /gene="argC"
                     /locus_tag="VF516_19845"
     CDS             complement(1148..>1999)
                     /gene="argC"
                     /locus_tag="VF516_19845"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEX8109997.1"
                     /translation="ARGCDVALLALPHKVTAAKVPELIAAGVKIVDMSGDFRLRDAAA
                     YEKYYGAKHPHPELLGTFVYGLPELHRAEIKRARYVASPGCFATAMELALLPLARAGL
                     LDRTTVHVTGITGSSGSGIAPGAGTHHPSRAGNLKTYKPLEHQHVPEVVQTLGDAGAK
                     DVQLRFVPVSAPLTRGIFATCFVELAADVDAARLAALFDESYAREPFTRRPRQRLPEV
                     VAVAGSNFIEVGFAVGPASGGKRTVTCFSAIDNLIKGGAGQAIQSMNLMLGCDERASL
                     EDVGNWP"
BASE COUNT          268 a          663 c          807 g          261 t
ORIGIN      
        1 gtcagcgagc tcatgctgtc gctccatcgt ctgaccgacg accccggata cgccgacttc
       61 gcgcgggtcc tcgccgacga catcgtccgc cgggcgaccc gccgcagcga cggcggcctc
      121 gaatgggtcc acgccgagaa ccgggtccag ccgtactggc gccagtcgta caccggctac
      181 atgcagggcg ccgccgggat cggcagcctg ttcatccgcc tcgccgcgca cgaccgccgg
      241 ctggggtgga aggtccggct gccggacaac ccgctgccga tcgtgtgacg cggcacctgg
      301 cctgggctca cggaggccgg tggtgctcag gcgtcggcga ccagcgtcgt gccgaccgag
      361 ccgggctcgc ggacggcgcg gaccaggtcg ccggcggcga gcttgccgag gatgtggatc
      421 gcgccgacgc cctgatcgat cacggccatc gcctcctcga gcttggcgat catgccgccg
      481 gtgacgacgc cgtcggcgat cgcgcggcgg gcgtcggcgc acgacaggcg ggtgagccgc
      541 gagccggggt cgttgacgtc gcgcagcacg cccggcgtgg aggtcaccag gaacaggcgc
      601 tcggcgcgca gcgcggcggc gagctggttg ccgaccaggt cggcgttgat gttgtagacg
      661 ccgccggccg cgtcggcgcc caggcaggcg atgaccggga ggtagcccgc ggaccacagg
      721 gtgtcgagca gcggcaggtg gaagccggtg acgtcgccga ccaggccgag atcgaccgga
      781 tcggggccgg cgctggggta ggccttgggc ggccggcgca cggcgcggat caggtggccg
      841 gacgcgccgt gcaggccgac cggggccagg ccggcggcga gcagggtggc gcacaggtcg
      901 acgttgagct tgccggcgag caccatcttc atgacgtcga gcgtggccgg atcggtgacg
      961 cgccggccgg cgacctggac ggtcgggatg ccgagccgct tctgcaggtc ggtggcctgc
     1021 gggccgccgc cgtggacgat cgcgatccgg gcgccgccgt cgaccagcgc cttgacgtcg
     1081 ccggcgagca cgccggcctc gccggagccg atcacctcgc cgccgatctt gatgatcgtg
     1141 ggacgggtca cggccagttc ccgacgtctt cgaggctggc gcgctcgtcg cagccgagca
     1201 tcaggttcat gctctggatc gcctggccgg cgccgccctt gatcaggttg tcgatcgccg
     1261 agaagcaggt gacggtgcgc ttgccgcccg aggccgggcc gacggcgaag ccgacctcga
     1321 tgaagttgga gccggcgacc gcgacgacct cggggagccg ctgccggggc cggcgggtga
     1381 acggctcgcg ggcgtaggac tcgtcaaaca gggcagcgag ccgggcggcg tcgacgtcgg
     1441 cggcgagctc gacgaagcag gtggcgaaga tgccgcgggt cagcggcgcg gacaccggga
     1501 cgaaccgcag ctggacgtcc ttcgcgccgg cgtcgcccag ggtctggacg acctcgggga
     1561 cgtgctggtg ctcgagcggc ttgtaggtct tgaggttgcc ggcgcggctg gggtggtggg
     1621 tgccggcacc gggggcgatg cccgagcccg acgagccggt gatgccggtg acgtggacgg
     1681 tggtgcggtc gagcaggccg gcgcgggcga gcgggagcag cgcgagctcc atcgcggtgg
     1741 cgaagcaacc gggggacgcg acgtacctcg cgcgcttgat ctcggcgcgg tggagctcgg
     1801 gcaggccgta cacgaacgtc ccgagcagct cggggtgcgg gtgcttcgcg ccgtagtact
     1861 tctcgtaggc ggcggcgtcg cgcagccgga agtcgccgga catgtcgacg atcttgaccc
     1921 cggcggcgat cagctcgggg accttggcgg cggtgacctt gtggggcagg gcgagcaggg
     1981 cgacgtcgca gccgcgggc
//
LOCUS       DASVOR010001030         4358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_478006,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001030 DASVOR010000000
VERSION     DASVOR010001030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4358
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478006"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VF516_19850"
     CDS             <1..307
                     /locus_tag="VF516_19850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8109998.1"
                     /translation="PRTVPLAGAAVAVGLAGAGVWSGLDTNKAHDAYAKNPTHEAFTE
                     GQSKQLRTNLLFGSAIAVGAATAVVAIWWTRWSGGETPAVALAPSSDGALVTYRGHF"
     gene            217..1806
                     /locus_tag="VF516_19855"
     CDS             217..1806
                     /locus_tag="VF516_19855"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8109999.1"
                     /translation="MDALERRRDARGRAGAVVRRRARHLQGTFLNELSNAAPLRLGRY
                     ELLARIATGGMGEIFLARLEGAAGFEKLYVIKRILPHLAGDSRFRAMLISEARIAARM
                     SHANICQVYELAETDHQLYIVMEYLEGITLLALLRKYAQQKRQLPFGFIAGVVHQVAE
                     GLHYAHELRDRNGGLMGVVHRDITPSNLFLTESGIAKVHDFGVAKAKDSTTTESGAVK
                     GKFAYMAPEQLAGHEVDRRADIFGLGVVITEMISNRRLFQRKTDYLTFQAVMEKPLPD
                     FRRHRPELPEAMVPVLTRALARDPADRHATIRELDAAVTGGLGRPWSQAELSELVRSD
                     FGEELQRHHSEVSGVIHRGSPRIMPTILESPSDPDAESYATFETSMASSGTEVSDAAP
                     IPQLGERVRTHWKLIAAAGGAVLLVVLALVTHGFRGSSRPPASPLVIDAAGPLAAYGE
                     AIRARNGELASCLARHDDAVPDGAQAVVRIGVDGHPRQVSFTPEQVERSGLAGCLRDV
                     LTATVFPPGAAEREIALGVRR"
     gene            complement(1816..2502)
                     /locus_tag="VF516_19860"
     CDS             complement(1816..2502)
                     /locus_tag="VF516_19860"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8110000.1"
                     /translation="MFSSDGMRPDLMERYVDKGLMPTYKDLIEHGVIGRNGLEQGFPP
                     NTGVGWHTLATGTWPGKHGSTNNTFYQTGDPFNSLRSLSDRGILQADTMQQASERAGK
                     KVAAVEWTGTINLVPAIQGPVVEYRTFFGGRGIVLNYDLSPQQPALANTFGVQYQKRT
                     LANAAGWTNVPASAPGGPAKETFFSQADNRIPGDGTWDVYIYDSTNDGRVNYDRILYQ
                     IVSEDHSQVH"
     gene            complement(2884..3018)
                     /locus_tag="VF516_19865"
     CDS             complement(2884..3018)
                     /locus_tag="VF516_19865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110001.1"
                     /translation="MHTLDAHSHDTMTLFFRMFLWIPQQASLESGSQASALGAFYGVM
                     "
     gene            3275..4216
                     /locus_tag="VF516_19870"
     CDS             3275..4216
                     /locus_tag="VF516_19870"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8110002.1"
                     /translation="MALPHTCGANRNDDCCTSLLVPGGDYFRSYDVGTDGQTRYGAIF
                     PATISSFRLDKYEVTVGRFRAFVDGSLATQSNAPAVGAGANAHVPGSGWQASWNQYLP
                     LDKKDFLNSARCAEGGMGATTMFDTWINNVGPNEDRPMNCVSWYEAMAFCAWDGGYLP
                     TEAEWNYAAAGGSEQRAYPWSDPPSSVAIDGSRGSYYDGSGCIGDGDPACTLADLLPV
                     GSKPAGDGRWGQSDLFGNVDEWVLDWFSDYRTPCTDCANLIQPIAAPDRVIRGSAFYA
                     SVGGHLHTGYRWGYPPGVSSSYGAGGYDVGFRCARAP"
BASE COUNT          691 a         1451 c         1498 g          718 t
ORIGIN      
        1 gccacggacg gtcccgctcg ccggcgcggc ggtcgccgtc gggctcgccg gtgccggcgt
       61 gtggtcgggg ctcgacacca acaaggcgca cgacgcctac gccaagaacc cgacgcacga
      121 ggcgttcacc gagggtcagt ccaagcagct gcgcaccaac ctcctgttcg gcagcgcgat
      181 cgcggtcggc gccgcgaccg cggtggtcgc gatctggtgg acgcgctgga gcggcggaga
      241 gacgcccgcg gtcgcgctgg cgccgtcgtc cgacggcgcg ctcgtcacct acaggggaca
      301 tttctgaacg agctgtccaa cgccgcgcct ctgcgcctcg gccgttacga gctgctggct
      361 cggatcgcca ccggaggcat gggcgagatc ttcctggccc ggctcgaggg cgccgccggg
      421 ttcgagaagc tgtacgtgat caagcggatc ctgccgcacc tcgcgggcga cagccggttt
      481 cgcgcgatgc tgatcagcga ggcgcggatc gcggcccgga tgtcgcacgc caacatctgc
      541 caggtctacg agctcgccga gaccgatcac cagctctaca tcgtgatgga gtacctcgag
      601 ggcatcacgc tgctcgcgct gctccgcaag tacgcgcagc agaagcgcca gctcccgttc
      661 ggcttcatcg ccggcgtcgt ccaccaggtc gccgagggcc tgcactacgc gcacgagctg
      721 cgcgaccgca acggcggcct gatgggcgtc gtccaccgcg acatcacgcc gtccaacctg
      781 ttcctgaccg agagcggcat cgccaaggtc cacgacttcg gcgtcgccaa ggccaaggac
      841 tcgacgacca ccgagtcggg cgcggtcaag ggcaagttcg cctacatggc accggagcag
      901 ctcgccggcc acgaggtcga ccgccgggcc gacatcttcg ggctcggcgt ggtgatcacc
      961 gagatgatct cgaaccgccg gctgttccag cgcaagaccg actacctgac gttccaggcc
     1021 gtcatggaga agccgctgcc cgacttccgg cggcatcgcc cggagctccc cgaggcgatg
     1081 gtcccggtcc tgacccgcgc gctcgcccgc gatccggcgg atcgccacgc gacgatccgc
     1141 gagctcgacg ccgcggtcac gggcgggctc ggccggccgt ggagccaggc cgagctcagc
     1201 gagctggtcc gcagcgactt cggcgaggag ctgcagcgcc accacagcga ggtctcgggc
     1261 gtgatccacc gcggctcgcc gcggatcatg ccgaccatcc tcgagagccc gtcggacccc
     1321 gacgcggaga gctacgcgac gttcgagacc agcatggcga gcagcggcac cgaggtgtcg
     1381 gacgccgctc cgatcccgca gctcggcgag cgcgtacgca cccactggaa gctgatcgcg
     1441 gcggcgggcg gcgcggtgct gctcgtggtg ctggcgctgg tgacccacgg gttccggggc
     1501 tcgtcgcgtc cgccggcgag cccgctggtg atcgacgcgg ccgggccgct cgcggcgtac
     1561 ggcgaggcga tccgggcccg caacggcgag ctcgcgagct gcctggcccg gcacgacgac
     1621 gcggtgcccg acggtgccca ggcggtggtg cggatcggcg tcgacggcca cccgcgccag
     1681 gtgtcgttca cgccggagca ggtcgagcgc tcggggctcg ccggttgcct tcgcgacgtg
     1741 ctgaccgcga ccgtgtttcc gccgggcgcc gccgagcgcg agatcgccct cggcgtgcgg
     1801 cggtgacggc acgggtcagt gcacctgtga gtggtcttcg ctgacgatct ggtagaggat
     1861 ccggtcgtaa ttcaccctgc cgtcgttcgt cgagtcgtag atgtagacgt cccaggtacc
     1921 gtcaccggga atccggttgt ccgcctggct gaagaacgtc tccttggccg ggccccccgg
     1981 agccgaggcg ggaacgttcg tccagcccgc ggcgttcgcc agcgtgcgct tctggtactg
     2041 gacgccgaag gtgttcgcca gcgccggctg ctgcggcgac aggtcgtagt tcaggacgat
     2101 cccgcggcct ccgaagaagg tccggtactc gacgaccggg ccctggatcg cgggcaccag
     2161 gttgatggtg cccgtccact cgaccgccgc gaccttcttg ccggcgcgct cggatgcctg
     2221 ctgcatcgtg tcggcctgga ggatccccct gtccgagaga ctcctgaggc tgttgaacgg
     2281 gtcaccggtc tggtagaacg tgttgttggt cgacccgtgc ttcccgggcc acgtcccggt
     2341 ggcgagcgtg tgccagccga cgccggtgtt cggcgggaag ccctgctcga ggccgttgcg
     2401 gccgatcacc ccgtgctcga tcagatcctt gtaggtgggc atgagcccct tgtcgacgta
     2461 gcgctccatc aggtccgggc gcatcccgtc ggaggagaac atgatcgctt ttcgcgaccg
     2521 tgacccatcc ttgtccctgc ccttgtcctg accggcagcg aggcctgcaa acactggcat
     2581 tgtgagcgcc gcgagcgcgg ctgcgacaag acctcgagat ttcttcacct gagccctctc
     2641 cctttctccc ctccacacgt gcgccggcgc tcccctgagg atctgatcca ggagcgaggt
     2701 gggctgcttg ctccagatga agcgctgcag ctcaccgact tcattggccg ccaagccgcg
     2761 cgtgggcgca caatggcaga gcgtcgagct cgtatcgaac gccagggtct attcactgca
     2821 taccatccag cacataggca atgagaggaa caagtgagat gcggaagcga cgtgtggcag
     2881 gtactacatg acgccgtaga acgcgccgag cgctgaggct tgcgagcctg attccaacga
     2941 ggcttgctgt ggaatccaga gaaacatgcg gaagaacaaa gtcatcgtgt cgtggctgtg
     3001 cgcgtcgagc gtgtgcatgg gcggatgccc gtcggcggcc tactttcagt gcgatcagag
     3061 ctctgtttgt gacctgcatc cgggcggccg gtgtaccgct gcgtcgtcgg ggagcatgtg
     3121 gtgctcctac ccggactcgt cgtgtccgag cggctaccgc tactcggatg tccacaccgg
     3181 cgatggaatc tccggacgat gcgtgcccat cgagtccggc gttgacgccg ggccgccgag
     3241 cgacgcggta gctgcagggc ccgcagttag ctgcgtggcg ttgccccata catgcggtgc
     3301 caaccggaac gatgactgtt gcacgagcct tttggttccc ggcggcgact acttccgcag
     3361 ctacgacgtc ggcaccgacg gtcaaacaag gtacggcgcg atctttcctg cgacgatcag
     3421 tagctttcgc ctcgacaagt acgaggtcac ggtgggccgt ttccgtgcgt tcgtcgacgg
     3481 gagcctggca acgcagagca acgcacctgc cgtaggtgcc ggtgcaaacg ctcacgtccc
     3541 tgggagcgga tggcaggcct cgtggaacca gtacctcccg ctggacaaga aggactttct
     3601 gaacagcgcc agatgcgccg agggaggcat gggcgcgacc acgatgtttg acacatggat
     3661 aaacaacgtc gggccgaatg aggatcggcc gatgaactgc gtctcctggt acgaagcgat
     3721 ggccttttgc gcatgggatg gtggatacct gccgacggag gcggaatgga actatgcggc
     3781 ggcgggtggc agcgagcaac gcgcgtaccc ttggtcggac ccgccctcat ccgttgcgat
     3841 cgatggttcg cgtggcagct actatgacgg ctccggttgc atcggcgacg gagatccggc
     3901 ctgcacgctt gcagacctgt tgccggtcgg ctcgaagccg gcgggagatg gcagatgggg
     3961 ccaatcagac ctgtttggaa acgtcgatga gtgggtgctc gattggttta gcgactatcg
     4021 gacgccgtgc accgactgcg cgaacctgat ccaacccatc gccgccccgg accgagtcat
     4081 ccgcggctcc gccttctacg cgagcgtagg cggacacctt cacaccggct acaggtgggg
     4141 ataccctccg ggggtgtcaa gctcgtacgg ggcgggcggg tacgacgtcg ggtttcgctg
     4201 tgctcgtgcg ccatgaccgc gtactgcgac gtcgccaaag gacacgggcg attccctcgg
     4261 cgtgcggcgg tgaccgcggt caatgcagaa ggaccagcac ccggcgctcg tgcgtcgagc
     4321 tcgccacggt caggtcgagg tactcgccgc ggcgcggg
//
LOCUS       DASVOR010001031         2458 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_215111,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001031 DASVOR010000000
VERSION     DASVOR010001031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2458
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215111"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..288)
                     /locus_tag="VF516_19875"
     CDS             complement(<1..288)
                     /locus_tag="VF516_19875"
                     /inference="COORDINATES: protein motif:HMM:NF017243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HEX8110003.1"
                     /translation="MSDWTVHLEFEEGASSKFWRARVEGKTLYVNYGKIGSNGQTQVK
                     DLADPAAAKKEYDKLVAEKRKKGYVDTGSGGRAPADEDGDEDAASNGADDED"
     gene            complement(320..1630)
                     /locus_tag="VF516_19880"
     CDS             complement(320..1630)
                     /locus_tag="VF516_19880"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEX8110004.1"
                     /translation="MATDALAWADVAAAGGIRGWVDAEVAHQGLADPGATSAMTDAEK
                     QLYKARRDEERKLRRELYRRAWQAYKRAHIVHLGPGVFWHDTADVDRFDADDPERRRQ
                     DSALPDLRDVEALARALGLTIPRLRWLAYHRDVDSGTHYIRWTVPKRDGSQRLISAPK
                     PELKAAQRWIAREVTEHLPVHGAAHGFLTGRSIASNAQVHAGARIVVKLDIRGFYPTV
                     TMRRVKGLLRRAGLGEQVATVMALLATESPREPVVTHGKTYHVAIGPRSLPQGAPTSP
                     SITNALCLRLDCRLSGLARELGCRYTRYADDLTFSWHGDARPQVGALLRGAGAIVRAE
                     GFEIHARKTRVMRAGARQKVTGLVVNPAPADRPTARVPRMTQRALRAAIRNRELGRPG
                     KGETLEQLRGMAAFVMMTDPARGRAFMARIERLIAARDRPRSEP"
     gene            complement(2065..>2458)
                     /locus_tag="VF516_19885"
     CDS             complement(2065..>2458)
                     /locus_tag="VF516_19885"
                     /inference="COORDINATES: protein motif:HMM:NF016330.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HEX8110005.1"
                     /translation="TLDVEQRTSAATCTCSAFRRAGIKEGPCEHMIALRVLYAREQAR
                     LEAARATPEGRASITAETRVLLRRTARGAELYRLSLDGRSVTARFGIGDALRMQRLRF
                     DSASDARAAYFARLDDLSRKGYLDATQD"
BASE COUNT          331 a          866 c          899 g          362 t
ORIGIN      
        1 gtcttcgtcg tccgcgccgt tggacgcggc gtcctcgtcg ccgtcctcgt ctgccggggc
       61 tcggccgccg gagccggtgt cgacgtagcc cttcttgcgc ttctcggcga cgagcttgtc
      121 gtactccttc ttcgccgccg ccggatcggc gaggtccttg acctgggtct ggccgttgct
      181 gccgatcttg ccgtagttga cgtacagcgt cttgccctcg acgcgcgcgc gccagaactt
      241 gctggaggct ccttcctcga actccaggtg cacggtccag tcgctcatgg gggtcctccg
      301 aggatggcag cgatggcgct catggctcgc tccgcggccg gtcgcgcgcc gcgatcagcc
      361 gctcgatgcg cgccatgaag gcgcggccgc gcgccgggtc ggtcatcatc acgaatgccg
      421 ccatgccccg gagctgctcc agggtctcgc ccttgccggg ccggccgagc tcgcggttgc
      481 ggatcgcggc gcgcagcgcg cgctgggtca tgcgcggaac gcgtgcggtc ggtcgatcgg
      541 cgggcgcagg gttgaccacc agcccggtga ccttctggcg cgccccggcg cgcatcacgc
      601 gggtcttccg ggcgtggatc tcgaagccct cggcgcggac gatcgcgccg gcgccgcgca
      661 gcagcgcgcc gacctggggc cgcgcgtcgc cgtgccacga gaacgtgagg tcgtcggcgt
      721 agcgcgtgta ccggcaaccg agctcgcgcg ccaggccgga cagccggcag tcgagccgca
      781 ggcacagcgc gttggtgatc gacgggctgg tcggcgcacc ctgcggcagc gagcgcggcc
      841 cgatcgcgac gtggtaggtc ttgccgtggg tcaccaccgg ctcgcgcggc gactcggtgg
      901 cgagcagcgc catcaccgtc gcgacctgct cgccgagccc ggcgcgccgg agcaggccct
      961 tgacccgccg catcgtcacc gtggggtaga acccgcggat gtcgagcttg accacgatgc
     1021 gcgcgccggc gtggacctgc gcgttgctcg cgatcgagcg gccggtgagg aagccgtgcg
     1081 ccgcgccgtg caccggcagg tgctcggtga cctcgcgggc gatccagcgc tgcgcggcct
     1141 tgagctcggg cttgggggcg ctgatcaggc gctgcgagcc gtcgcgcttg ggcaccgtcc
     1201 agcggatgta gtgggtcccg ctgtcgacgt cgcggtggta ggccagccag cgcagccgcg
     1261 ggatggtcag gccgagcgcc ctggcgagcg cctcgacgtc gcgcagatcc ggcagcgcgc
     1321 tgtcctgccg ccggcgctcg ggatcgtcgg cgtcgaaccg atcgacgtcc gcggtgtcgt
     1381 gccagaacac gccggggccg aggtggacga tgtgggcgcg cttgtaggcc tgccaggcgc
     1441 gccggtacag ctcgcggcgc agcttgcgct cctcgtcgcg gcgcgccttg tacagctgct
     1501 tctcggcgtc ggtcatcgcc gacgtggcac ccggatcggc gagcccctgg tgcgcgacct
     1561 cggcgtcgac ccagccgcgg atgccgccgg ccgccgcgac gtcggcccac gcgagcgcgt
     1621 cagtcgccat gcgagccctc cgggacacgc ggcgcccagg gccagatcaa aggcgaggac
     1681 aaaagcgagg ccaaaggcga ggacaaaggc gaggacaaaa gcgaggccaa aagcgaggag
     1741 gagtcgaatc cgctacatcc ctcatgggac acggtaggct caccggtctg ccgccctgaa
     1801 ggggtactgc gtatgggtgc aaaccgcggt cgtgtggttg gtccagctgc gcccgtacgc
     1861 agtacccctt cagggcgagc agaccaagaa cagcctacct tgtggagagt ggccactgat
     1921 tgtgcccgga cggcgcgacg ccgcccttgc gccggagcgc aggctaacgg attcgactcc
     1981 tcctcggaaa tcaagattga cgcacctccc ggtggaattc catcgttcgc gctccaaatc
     2041 gcggagccgg ggcggcccgg agcgtcagtc ctgggtcgca tcgagatatc ctttccgtga
     2101 cagatcgtcg agccgcgcga agtacgcggc gcgcgcgtcc gacgccgagt cgaaccgcag
     2161 gcgctgcatg cgcagcgcgt cgccgatccc gaaccgggcg gtgaccgacc ggccatcgag
     2221 cgacagccgg tagagctcgg caccgcgcgc ggtgcgccgc agcagcacgc gggtctccgc
     2281 cgtgatcgac gcgcggccct ccggcgtggc gcgcgcggcc tcgagccggg cctgctcgcg
     2341 cgcgtagagc acgcggagcg cgatcatgtg ctcgcaaggg ccctccttga tcccggcgcg
     2401 gcgaaacgcg ctgcatgtgc aggtcgcggc ggacgtccgc tgctccacgt cgagggta
//
LOCUS       DASVOR010001032         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_331673,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001032 DASVOR010000000
VERSION     DASVOR010001032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331673"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF516_19890"
     CDS             complement(<1..327)
                     /locus_tag="VF516_19890"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX8110006.1"
                     /translation="MLAGDVLDRIPTEPGVYLFKDARGAVIYVGKAKNLRARVRQYFR
                     DGGDERFFVAAGFLAKAVADVETIVVSSSKEALLLENHLIKKHQPRFNVKLRDDKQYL
                     VLRLVDP"
     gene            complement(377..1465)
                     /locus_tag="VF516_19895"
     CDS             complement(377..1465)
                     /locus_tag="VF516_19895"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX8110007.1"
                     /translation="MLTPLFLAIAIAALGFLIVVHEGGHYFVARWCRMRIERFSIGFG
                     PGLLKRTSKKTGTTFQLAPIPFGGFVEIRGMNIAEDVDPDDKYAYPNRPVWQRFITIF
                     AGPATNYLSAIALAFALYAVHGMDQPHWYGVGSVVADRHDIHDKHFDAYGRLEPGDRI
                     LKFNGQPLFIDSRPNLTERINQSKDAPVTLTIERDGRTFDVRLRPQQGKDGAGHVGWM
                     IGVNLEHQDLVVGVSLGEAARQAVIYPVEQTRMIGRVLYGVVFGSEKADPGGPIRMVE
                     EFYKAFSTSIVDGIKLLMLLSVYLGLFNLLPIPALDGGRLVFLVYEMVTRRRANPKIE
                     TMVHMAGIMALGVVMLVVLYIDIRRVFT"
     gene            complement(1528..>1863)
                     /locus_tag="VF516_19900"
     CDS             complement(1528..>1863)
                     /locus_tag="VF516_19900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110008.1"
                     /translation="GVDIGAGRAQGTLLLIATPDVASASLDGAGDACRRLLAGEGDGE
                     VALGRYTVVHRMRALAAVRGTRLIGAAMHRRLSKRLTMQRLTVRISGPTGAVAKSAVG
                     RLRAFARFA"
BASE COUNT          303 a          633 c          644 g          283 t
ORIGIN      
        1 cggatcgacc agccgcagca ccaggtactg cttgtcgtcg cgcagcttga cgttgaaccg
       61 cggctggtgc ttcttgatca ggtggttctc gaggagcagc gcctccttgg acgagctgac
      121 cacgatggtc tcgacgtcgg ccaccgcctt ggcgaggaag cccgcggcga cgaagaaccg
      181 ctcgtcgccg ccgtcgcgga agtactggcg gacccgcgcg cgcaggttct tggccttgcc
      241 gacgtagatc accgccccgc gggcgtcctt gaacaggtac acgcccggct cggtgggaat
      301 ccgatcgagg acgtcgccgg ccagcacgag gtgaccttac cgcggcacgc gacagctgta
      361 ggtcaggtat cggccctcag gtgaacacgc ggcggatgtc gatgtagagc acgaccagca
      421 tcacgacgcc gagcgccatg atccccgcca tgtggaccat ggtctcgatc ttggggttgg
      481 cgcgccggcg ggtcaccatc tcgtagacca ggaacaccag gcggccgccg tcgagcgccg
      541 ggatcggcag caggttgaac agcccgaggt agacgctgag cagcatcagc agcttgatgc
      601 cgtcgacgat actggtcgag aacgccttgt agaactcctc gaccatgcgg atcgggccgc
      661 ccggatcggc cttctcgctg ccgaacacca cgccgtacag cacgcggccg atcatccggg
      721 tctgctcgac cggatagatc acggcctgcc gggcggcctc cccgaggctg accccgacca
      781 cgagatcctg gtgctcgagg ttcacgccga tcatccagcc gacgtggccg gcgccgtcct
      841 tgccctgctg cggccgcagc cggacgtcga acgtcctgcc gtcgcgctcg atcgtcaggg
      901 tgaccggcgc gtccttggac tggttgatcc gctcggtcag gttcggccgg ctgtcgatga
      961 acagcggctg cccgttgaac ttgaggatgc gatcgccggg ctcgagccgg ccgtaggcat
     1021 cgaagtgctt gtcgtggata tcgtggcgat ccgccaccac cgagccgacg ccataccagt
     1081 gcggctggtc catgccgtgg accgcgtaga gcgcgaacgc gagcgcgatc gccgacaggt
     1141 agttggtcgc cgggccggcg aagatcgtga tgaaccgctg ccacaccggg cggttggggt
     1201 aggcgtactt gtcgtcgggg tcgacgtcct cggcgatgtt catgccgcgg atctcgacga
     1261 acccgccgaa cgggatcggc gcgagctgga acgtcgtgcc ggtcttcttc gacgtccgct
     1321 tgagcaggcc gggtccgaac ccgatgctga accgctcgat ccgcatccgg caccagcgcg
     1381 cgacgaagta gtggccgccc tcgtggacga caatcaggaa gccgagtgcg gcgatcgcga
     1441 tggccaggaa caggggcgtg agcacgcact cttactatag caacgaaacg cgcggtcgcg
     1501 cccggagctc tgcgggccgt gagaaaatca cgcgaaccgg gcgaacgctc gcagccggcc
     1561 gaccgcggac ttggccaccg caccggtcgg gcccgagatc cggaccgtga ggcgctgcat
     1621 cgtcaggcgc ttgctcagcc tgcggtgcat tgccgcgccg atcaggcggg tgccgcggac
     1681 cgcggcgagc gcgcgcatgc ggtgcaccac ggtgtaccgg ccgagcgcga cctcgccatc
     1741 gccctcgccg gcgagcaggc ggcggcacgc gtcgcccgcg ccgtcgagcg acgcgctcgc
     1801 gacgtccgga gttgcgatca gcagcagcgt gccctgggcg cggccggcgc cgatgtcgac
     1861 ccc
//
LOCUS       DASVOR010001033         4272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_302782,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001033 DASVOR010000000
VERSION     DASVOR010001033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302782"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VF516_19905"
     CDS             <1..223
                     /locus_tag="VF516_19905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8110009.1"
                     /translation="KFDSSVDRGQPFSFPLGQGRVIKGWDEGVATMKIGGKRTLLIPP
                     HLGYGDRGAGGKIPPGATLLFEVELLGVR"
     gene            complement(142..552)
                     /locus_tag="VF516_19910"
     CDS             complement(142..552)
                     /locus_tag="VF516_19910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110010.1"
                     /translation="MRWFAWLLVAACSGRAGGAAHPASPPAAQSAPPPADDAPSEAEC
                     DQLITHAVALGINEQGRMEAPRSTESDHEAVRRSLHDDFVAGCRTLPRDAYRCAMAAT
                     TLAALAACQRTPSSSTSNSSVAPGGILPPAPRSP"
     gene            579..1388
                     /gene="uppP"
                     /locus_tag="VF516_19915"
     CDS             579..1388
                     /gene="uppP"
                     /locus_tag="VF516_19915"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HEX8110011.1"
                     /translation="MALWFAALLGLVQGLTEYIPVSSTAHLRVVSALARQGDAGAAYT
                     AVIQLGTLAAVLAYFAKDLVALAVAMVRAPDSHDGRMPWLIAVGTVPIVILGLVLKKH
                     IETDLRSLYVIAATLITIGIVMAVIDRHAVGRDGRTLETITYQDAILIGLAQTLALVP
                     GVSRSGATICMALLLGFTRSDAARFSFLLSIPAVAGAGILEARKAFTELGSSAIPALA
                     VGTGVAAVVGYASIAWFLRWLGSHQLIGFAVYRIAAGLALLGALAARIVSA"
     gene            1622..3709
                     /locus_tag="VF516_19920"
     CDS             1622..3709
                     /locus_tag="VF516_19920"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8110012.1"
                     /translation="MEIDSAPQGAAIYVNSKSCPPLGVTPWAGKLNAGDYTIIFEAPG
                     YEPASKPFRVARLRKAQQLFAPLVKKAEPPKIDVRADADKNVAGATVSLDGQPQGQAP
                     MVLTTSAGRHLVALKKDGFEEYSAWLEVKDNQVQTFAPTIKEIAKPKYGTVVVDSDVA
                     DAEVYIDGNKHPDNTPAVISNVIEGLHVIEVRKAPGLPWKQTVEVKASQQTKVRAELA
                     AAMNGGVGVIRVLSDAQGARAFIDGTDMGPVPVDIKDIKAGDHIVQVKAQGFQPSERH
                     VTVTAGGSQIVKFDLNTEAPGDQGLLTVHSNMPKAMVSIDGAEVGMAPQEKKKVAPGM
                     HSVLVRLDGYKQFEQKIQVDVGQTATVQADLKAVGRLRVLSTPPHATVMINGLPVGPT
                     PLDTEVEVGETVVRIEQPGFQPFEQTLTIQGGKTEVLTRELAVAGPSEAELAVEQRAL
                     SSFGARVLPRGRSTVDVDVGYPYFTTARITVGAGRIPNFFGFDASVGVRSMLSRTEIG
                     LGGRMTVVDNEPFSAAAFTDLWWGSKLLDDSKRNGVTWDLGGIVSLTALTHVTISGRA
                     YVELWSDRHCPAIDTSMPNHFEGTDPIQVCTQVKDGSISATDLARVRKLIGSDVMTGE
                     SSTLFNRENGARFLLSLTGEIAFQQEWNVFFILEGAPFQQSERALFTNLFSGSMPTND
                     YRIYARTGLTYKF"
BASE COUNT          631 a         1425 c         1578 g          638 t
ORIGIN      
        1 caagttcgac agctcggtcg accgcgggca gccgttctcg ttcccgctcg gccagggccg
       61 cgtcatcaag ggctgggacg agggcgtcgc gacgatgaag atcggcggca agcggacgct
      121 cctgatcccg ccgcacctcg gctacggcga ccgcggcgcg gggggcaaga tcccgccggg
      181 cgcgacgctg ctgttcgagg tcgagctgct cggcgtgcgc tgacaggcgg cgagcgcggc
      241 gagcgtggtg gcggccatcg cgcagcggta ggcgtcgcgc ggcagcgtgc ggcagcctgc
      301 cacgaagtcg tcgtgcagcg agcggcggac cgcctcgtgg tcggactcgg tgctgcgcgg
      361 cgcctccatg cggccctgct cgttgatgcc gagcgcgacg gcgtgggtga tcagctggtc
      421 gcactccgct tcactcggcg catcgtcggc gggcggcggg gcggattgcg cggcgggcgg
      481 ggacgcgggg tgtgcggcgc cgcccgcacg gccggagcac gcagcaacca ggagccaggc
      541 gaaccatcgc acggtgcatc gatgttagcg tgtccggcat ggcgttgtgg ttcgctgcgc
      601 tgctgggcct ggttcaaggc ctcaccgagt acatcccggt ctcgtcgacc gcccacctcc
      661 gggtggtgtc ggcgctcgcc cgccagggcg acgcgggagc cgcgtacacc gcggtgatcc
      721 agctcggcac gctggcggcg gtgctcgcgt acttcgcgaa ggacctggtc gcgctcgcgg
      781 tcgccatggt ccgcgcgccc gacagccacg acggccggat gccgtggctg atcgcggtgg
      841 ggacggtgcc gatcgtgatc ctcgggctgg tgctcaagaa gcacatcgag accgacctgc
      901 ggtcgctgta cgtgatcgcc gcgacgctga tcacgatcgg catcgtgatg gcggtgatcg
      961 atcgccacgc ggtggggcgc gatggccgca cgctggagac gatcacgtac caggacgcga
     1021 tcctgatcgg gctggcccag accctggcgc tggtccccgg cgtgtcgcgc tcgggggcga
     1081 cgatctgcat ggcgctcttg ctcgggttca cgcgcagcga cgcggcgcgg ttctcgttcc
     1141 tgctctcgat ccccgcggtc gccggggccg ggatcctgga ggcgcgcaag gccttcaccg
     1201 agctgggcag ctcggccatt ccggcgctcg cggtcgggac cggcgtggcg gcggtggtgg
     1261 gatatgcgtc gatcgcgtgg ttcctgcgct ggctgggctc gcaccagctg atcgggttcg
     1321 cggtgtaccg gatcgccgcc gggctcgcgc tgctcggcgc gctcgccgca cgaattgttt
     1381 cagcctaggt gccgcccccc tgccaggcgg tgtgaaaacg cgcacgcgac ggcgcgcgac
     1441 gggcgggact gctggtaggc ttgcgtccgt ccgtccattg agggtgccga tgacgattgc
     1501 tcgtctgcgt gttgtcttgc cctggtcgct gacgctggtg ctcgccgtcg cagcgtggtc
     1561 gcgcgccgcg catgcccagg gtgcctgtcc cggcctcgat ccgaagcata caaggtacgc
     1621 ggtggagatc gactcggccc cgcagggcgc ggcgatctac gtcaacagca agtcgtgccc
     1681 gccgctcggc gtgacgccgt gggcgggcaa gctcaacgcg ggcgactaca cgatcatctt
     1741 cgaggccccg gggtacgagc cggcgtcgaa gccgttcagg gtcgcccggc tgcgcaaggc
     1801 ccagcagctg ttcgcgccgc tggtcaagaa ggccgagccg cccaagatcg acgtccgcgc
     1861 cgacgccgac aagaacgtgg ccggcgcgac cgtgtcgctc gacgggcagc cccagggcca
     1921 ggcgccgatg gtgctgacca ccagcgccgg ccgccacctg gtggcgctca agaaggacgg
     1981 cttcgaggag tactcggcgt ggctcgaggt caaggacaac caggtccaga ccttcgcgcc
     2041 gacgatcaag gagatcgcca agcccaagta cggcaccgtg gtggtcgact ccgacgtcgc
     2101 cgacgccgag gtctacatcg acggcaacaa gcaccccgac aacacgcccg cggtgatcag
     2161 caacgtgatc gaggggctgc acgtgatcga ggtccgcaag gcgccggggc tgccgtggaa
     2221 gcagacggtc gaggtcaagg ccagccagca gaccaaggtg cgggcggagc tcgcggcggc
     2281 gatgaacggc ggggtcggcg tgatccgcgt gctgagcgac gcgcagggcg cgcgggcgtt
     2341 catcgacggc accgacatgg ggccggtgcc ggtcgacatc aaggacatca aggccggcga
     2401 ccacatcgtc caggtcaagg cgcaggggtt ccagcccagc gagcgccacg tcaccgtgac
     2461 cgccggcggc tcgcagatcg tcaagttcga cctcaacacc gaggcgccgg gcgaccaggg
     2521 cctgttgacg gtgcactcga acatgcccaa ggcgatggtc agcatcgacg gcgccgaggt
     2581 cggcatggcg ccgcaggaga agaagaaggt cgcgccgggc atgcactcgg tgctggtccg
     2641 gctcgacggc tacaagcagt tcgagcagaa gatccaggtc gacgtcggcc agaccgcgac
     2701 cgtgcaggcc gatctcaagg cggtcggccg gctgcgcgtg ctgtcgacgc cgccccacgc
     2761 gacggtgatg atcaacggcc tgccggtggg gccgacgccg ctcgacaccg aggtcgaggt
     2821 cggcgagacc gtggtgcgga tcgagcagcc ggggttccag ccgttcgagc agacgctgac
     2881 catccagggc ggcaagaccg aggtgctgac ccgcgagctc gcggtcgcgg ggccgagcga
     2941 ggccgagctc gcggtcgagc agcgcgcgct gtcgtcgttc ggcgcgcggg tgctgccgcg
     3001 cggccgctcg acggtggacg tcgacgtcgg ctacccgtac ttcacgaccg cgcggatcac
     3061 ggtcggcgcc ggccggatcc cgaatttctt cgggttcgac gcctcggtcg gcgtgcgctc
     3121 gatgctgtcg cgcaccgaga tcggcctcgg cggccggatg accgtcgtcg acaacgagcc
     3181 gttctcggcc gcggcgttca ccgacctgtg gtggggcagc aagctgctcg acgactccaa
     3241 gcgcaacggc gtgacctggg acctcggtgg catcgtctcg ctgaccgcgc tgacccacgt
     3301 gacgatcagc ggccgggcct acgtcgagct gtggagcgac cggcactgcc cggcgatcga
     3361 cacgtcgatg cccaaccact tcgagggcac cgatccgatc caggtgtgca cccaggtcaa
     3421 ggacgggtcg atcagcgcga ccgatctcgc gcgggtccgg aagctgatcg gaagtgacgt
     3481 catgaccggg gagagctcga cgctgttcaa tcgcgagaac ggcgcgcgct tcctgctcag
     3541 cctgaccggc gagatcgcct tccagcagga gtggaacgtg ttcttcatcc tcgagggagc
     3601 gccgttccag cagagcgagc gggcgctgtt caccaacctg ttttccggat cgatgccgac
     3661 caacgactac cggatctacg cgcgcaccgg cctgacctac aagttctgac ctgccagggc
     3721 ctcggcggtg cgtttcgccc atcgaggcgc agcggcggct cagaagtagt agtaccgacg
     3781 cgctgtcgga gccctgccgg agccgctgcc ggaaccgctg tcgctgcccg agccggagcc
     3841 gctgcccgag cccgaaccgt cgaccggtgg gttgtcctcc tggaccggct tgcaggtcgg
     3901 gtacatgcca ttcgcgggca gctggacgtc gccgtagtcg ctcttcggcg gcgggtcgcc
     3961 cttcttgcag tacttggatc gcgccgatcc gggtggtcgc ggccagcccg gtcgcggcga
     4021 tcagcgggac gccgagcacg atcgcgtggg cgagcgcgtt gcggttgccg gccagcccgg
     4081 cgggcggtag cgtggacagg gtgaagccgg cgtgggtcac ggcgatcgcc gacgcggcga
     4141 gccaggtcag gtgttttctg gagtcgggct cgaggccctg cttcggggat ggcaagcgcg
     4201 gcgtggattc gccagcccca tgcctctcta gcgacagcgc cgggcgaagt ccgaatcttc
     4261 gaggaactga tc
//
LOCUS       DASVOR010001034         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_244241,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001034 DASVOR010000000
VERSION     DASVOR010001034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244241"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VF516_19925"
     CDS             complement(<1..833)
                     /locus_tag="VF516_19925"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8110013.1"
                     /translation="MEVRGSITDRPWGLTLGAIALRRHTGQLTIDSDGKRYCIAFEDG
                     AVVAASSPQAADAIARIALTNHFITSTQVADITRRLAMSPDRDEVEVLADVIRFTPDQ
                     ALKLRHRLVAQRAARTFSLERGEFVLDDRIALPMYPGVAVDHRHVIYLGVRTNLAEQR
                     LIDDLRQLGAYFVLKPEVIDELPRYGFEPAAHTVLEALKLGTSLPELEASHREVDPRF
                     AQAIIYALVSCTACAIPPRTGQPAAPPAAPRPTIPTHDATPRLARRSLHPAPPGHAPP
                     G"
     gene            1204..>3172
                     /locus_tag="VF516_19930"
     CDS             1204..>3172
                     /locus_tag="VF516_19930"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110014.1"
                     /translation="MRKSIAIAAAAVVVSLAHPTRAATLEEILAKNLAARGGEAKLRE
                     IKTLRLTGRFVFGGGDFSVEAAWGQIQKRGTGNAGQIRSEVTLQGLTQITAFDGHEGW
                     NLSPFQGRREPEKASEDEARSLAQQAELDGPLIGWRDKGHRVEYLGTEDSDGTPAIKL
                     RVTRKDGDLQYVYLDPDSYLEIRITTVHKVRGAERITETDLGGYQQVNGVWLPFALET
                     GGKGGPRNLHITIERADANVAADDAWFKLPAPRTPIAAVITAGPGEPRAIAATVAPPA
                     PTEKAILDGGVISGLGARNIGSATMSGRIAAVAGRIVGGKTLLYVGAASGGVWKSQDG
                     GTTFKPVFDKQPVQSIGAIAIDPSNPETVWVGTGEAWTRNSTSIGNGIYKSTDGGETW
                     TNMGLPGSERIVRIAVHPTASQVVYACVPGKLWSDSNDRGLYKTTDGGKTWTLVLKGQ
                     NPSTGCSSVTMDPRNPEVLIAGLWDFRRKGWTFRSGGDGPSAPSASGMFRSTDGGKTW
                     ASLATAKGLPPAPWGRVETVFAPSDSKIVYALIESKASALFRSADGGATWEQRDTSQM
                     MVWRPFYFARLIVDPTNPNRLFKPDLRLAVSEDGGKSFADTGGGAHGDWHDLWIEPTN
                     PKHVIGGDDGGLWLSYDGGNRWWKGQNLPISQ"
BASE COUNT          518 a         1074 c         1150 g          430 t
ORIGIN      
        1 tggccaggtg gagcatggcc aggtggagca ggatgaaggc tgcggcgggc gagccgcggg
       61 gtcgcgtcgt gcgtcgggat cgtcggtcga ggcgcggcgg gcggcgccgc cggctggccg
      121 gtgcgcggcg ggatcgcgca cgcggtgcac gacaccagcg catagatgat cgcctgcgcg
      181 aaccgcggat cgacctcgcg atgcgacgcc tccagctcgg gcaggctggt gccgagcttg
      241 agcgcctcga gcacggtgtg cgcggcgggc tcgaagccgt agcgcggtag ctcgtcgatc
      301 acctcgggct tgagcacgaa gtacgcgccg agctggcgca ggtcatcgat cagccgctgc
      361 tcggcgaggt tggtccgcac cccgaggtag atcacgtggc ggtggtcgac ggcgacgccg
      421 gggtacatcg gcagcgcgat gcgatcgtcg agcacgaact cgccgcgctc cagcgagaac
      481 gtgcgcgccg cgcgctgggc gaccagccgg tggcggagct tgagcgcctg gtcgggcgtg
      541 aaccggatca cgtccgcgag cacctcgacc tcgtcgcggt cgggcgacat cgccaggcgt
      601 cgggtgatgt cggcgacctg ggtcgaggtg atgaagtggt tggtcagcgc gatccgcgcg
      661 atcgcgtcgg ccgcctgggg agagctcgcg gcgaccaccg caccgtcctc gaacgcgatg
      721 cagtagcgct tgccatcgct gtcgatggtc agctggccgg tgtggcggcg cagggcgatc
      781 gcacccaggg tcaagcccca ggggcgatcg gtgatgctgc cgcgcacctc catcaccgca
      841 ccgtcagcac gcgggccatg ccctatttga cggagttttc aggcctcgcg caatgcgcgt
      901 gcaaggggtg ggtctcacgg tgtagccacc tgaaactcat cccgaaaaag actccgatag
      961 tttcggccat ccagcgtgat tcctgcgccg gatcggccgc gagatcacca cgcccttcca
     1021 tatgaggagc agctcgattc accgcggcat ggcgcgttcc tcgggaggaa atgctcccgc
     1081 cgccgcgtgt aggtggacat gcgtgtgtcg caccaggacg tactgggaca agctgcgaca
     1141 actaaggtgc cgtgactact tgctatttca tctaaccaga ggtgcgcgcg atactccccg
     1201 gccatgcgga aatcgatcgc gattgcagct gccgcggtgg tggtatcgct ggcacacccc
     1261 acgcgggcgg ccaccctcga ggagatcctc gccaagaacc tcgccgcgcg cggaggtgag
     1321 gccaagctcc gcgagatcaa gacgctgcgc ctgaccggcc gattcgtgtt cggcggcggc
     1381 gacttcagcg tcgaagcggc gtggggtcag atccagaagc gcggcaccgg caacgccggc
     1441 cagatccgca gcgaggtcac gctgcagggg ctgacccaga tcaccgcctt cgacggccac
     1501 gagggctgga acctctcgcc gttccagggc cggcgcgagc cggagaaggc gtcggaggac
     1561 gaggcgcgct cgctcgccca gcaggccgag ctcgacggcc cgctgatcgg ctggcgcgac
     1621 aagggccacc gcgtcgagta cctcggcacc gaggactccg acggcacgcc ggcgatcaag
     1681 ctgcgcgtga ctcgcaagga cggcgacctg cagtacgtct acctcgatcc ggattcgtac
     1741 ctggagatcc ggatcaccac ggtccacaag gtccgcggcg ccgagcggat caccgagacc
     1801 gacctcggcg gctaccagca ggtcaacggc gtgtggctgc cgttcgcgct ggagaccggc
     1861 ggcaagggcg gaccgcgcaa cctccacatc acgatcgagc gcgccgacgc caacgtcgcc
     1921 gccgacgacg cgtggttcaa gctgccggcg cccaggaccc cgatcgcggc ggtgatcacc
     1981 gccgggcccg gcgagcccag ggcgatcgcc gccacggtcg cgccgcccgc gccgaccgag
     2041 aaggcgatcc tcgacggcgg cgtgatctcc gggctcggcg cgcgcaacat cggctcggcg
     2101 acgatgagcg gccggatcgc ggcggtcgcc gggcggatcg tcggcggcaa gaccctgctc
     2161 tacgtcggcg ccgcgtcggg cggcgtgtgg aagtcgcagg acggcggcac gacgttcaag
     2221 ccggtgttcg acaagcagcc ggtgcagtcg atcggcgcga tcgcgatcga cccgagcaac
     2281 cccgagaccg tgtgggtcgg caccggcgag gcgtggaccc gcaactcgac gtcgatcggc
     2341 aacgggatct acaagtcgac cgacggcggc gagacctgga cgaacatggg gctgcccggg
     2401 tccgagcgga tcgtgcggat cgccgtccac ccgacggcga gccaggtggt ctacgcctgc
     2461 gtgccgggca agctgtggag cgactcgaac gaccgcggcc tgtacaagac caccgatggc
     2521 ggcaagacct ggacgctggt gctcaagggc cagaacccgt cgaccggctg ctccagcgtg
     2581 acgatggacc ccaggaaccc cgaggtgctg atcgccgggc tgtgggactt ccgccgcaag
     2641 ggctggacgt tccgctccgg cggcgatggg cccagcgcgc cgagcgccag cgggatgttc
     2701 cgctcgaccg acggcggcaa gacctgggcg tcgctcgcga ccgccaaggg cctgccgccg
     2761 gcgccgtggg gccgggtcga gaccgtgttc gcgccgtcgg actccaagat cgtctacgcg
     2821 ctgatcgagt ccaaggcgtc ggcgctgttc cgctcggccg acggcggcgc gacctgggag
     2881 cagcgcgaca ccagccagat gatggtgtgg cggccgttct acttcgcgcg gctgatcgtc
     2941 gacccgacca accccaaccg gctgttcaag cccgacctcc ggctcgccgt cagcgaggac
     3001 ggcggcaaga gcttcgccga caccggcggc ggcgcgcacg gcgactggca cgacctgtgg
     3061 atcgagccga ccaaccccaa gcacgtgatc ggcggcgacg acggcgggct gtggctgtcc
     3121 tacgacggcg gcaaccggtg gtggaagggc cagaaccttc cgatctcgca gt
//
LOCUS       DASVOR010001035         3206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_302792,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001035 DASVOR010000000
VERSION     DASVOR010001035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_302792"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..>3206)
                     /locus_tag="VF516_19935"
     CDS             complement(29..>3206)
                     /locus_tag="VF516_19935"
                     /inference="COORDINATES: protein motif:HMM:NF036757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110015.1"
                     /translation="PVRAQPPVVMLRREKYDEQQLRFSPFEHTATCRFIMKLRDAGID
                     GLLDLATTRPPPRFKEAIDHRLDDKGKWHPLRPTWFERTYAPGPLVDTRNPPDFDVDF
                     EHDGPYSLYNWELFFHAPLQVAVRLAKDGRHEEAQRWFHFIFDPTTDASSPPPQRYWR
                     FAPFHENDDYAGAREIMSLLSYSGDDPVRLRRQQRVIDQVTAWWEKPFSPHVIARLRT
                     VAYQKAVVMKYIDNLIEWGDKLFRQDTLETIQEATQLYILAGNILGPRPERIPPLVEP
                     DPLTFGEVRKKLDLFANYEIRFESLQGLNQRVRRPFRIAARPDVSGARSVLSMVTQYF
                     CLPGNPQLDKYWDTVADRLFKIRNCMNIQGIVRQLPLFEPPIDPGLLVRAAAAGVDLG
                     SVIASLNAPPPHHRFRYLLARALRLAEELRSFGAMTATVLERRDAEGLAALRASGETA
                     LLEAVRDIRKKQVRQVEEQLNQLSLQREQLEIEIQYYDAQLQQLMNPQETASLQSLSL
                     SQVQAAIAEGQNLVAKVMHAIPDFQTGTAGGFSSPFVTLKLGGKMLGDIASAFAESVQ
                     KVMAKNETEADMAEKQAEYQRRREEWQHEKDLRLKEEAQLDKQIAEVNLKLEIASAEL
                     HSHDVDVENAHKVERYLRDKYTNEQLYGWMLGQLSTVYFQAYKVAFDAAQQAERAYRF
                     ERGEPSSSFIEFSYWDSLKKGLFAGERLLVDLRRMESAYLEVDRRALEVVRNVSLRED
                     VPLALEELLATGRCQIEITEGLLDGDFPGHYFRRVKTVSLTAVGGTRPHGNVNCTLTL
                     LENRIRTDANASGSYAQTEDTEDPRFLINVAPVQAVATSRPTADAGVFELRFDEDRYL
                     PFEGAGAVSTWRIDLHQADNALDLGELDDLIVTLAYTARNGGAALEASARASREKGLA
                     RGGAKPPAQHTLSLKRDLAAQWKRLAEATPGQEVEIPLPLEPERFSGRYRGLDLRVER
                     ATFFARARGPVPEDVLRLRLDPPKGSGAPAAGWTRPWPASQSLRASAEVSGPPGAWKL
                     VVSATRGKVPDYVADLVLVFDLRARKAT"
BASE COUNT          474 a         1114 c         1072 g          546 t
ORIGIN      
        1 ccactcccgt tcaaggacat tgcaaatatc aagtcgcctt tcgcgcgcgc aggtcgaata
       61 cgagcaccag atcagcgaca taatccggaa cttttccgcg cgtggcggaa actacgagtt
      121 tccacgcgcc gggcggtccg gacacctcgg cactggcccg gagcgactgc gacgccggcc
      181 agggtcgcgt ccagccggcc gccggcgcgc cggatccctt gggcggatcg agccgcaggc
      241 gcaggacgtc ctccggcacc ggcccgcgcg cgcgcgcgaa gaacgtcgcg cgctcgacgc
      301 gcagatcgag cccgcggtaa cgcccggaga agcgctccgg ctccagaggc aggggaatct
      361 cgacctcttg cccgggagtc gcctcggcca gccgcttcca ctgggcggcg agatcgcgct
      421 tcaagctgag ggtgtgctgg gccggcggct tcgcgccgcc gcgggccagc cccttctctc
      481 gactggcccg cgccgacgcc tccagggcag cgccgccgtt gcgcgccgtg taggcgagcg
      541 tcacgatcag atcgtcgagc tctccgagat ccagcgcgtt gtccgcctgg tgcaggtcga
      601 tccgccaggt cgagaccgcc cccgccccct cgaacgggag gtagcggtcc tcgtcgaagc
      661 gcagctcgaa cacgccggcg tcggcggtgg gccggctggt ggccaccgcc tgcacgggcg
      721 cgacgttgat caggaaccgc ggatcctccg tgtcctccgt ctgtgcgtag gagccgctgg
      781 cgttggcgtc ggtccggatt cgattctcca gcaacgtcag cgtgcaattg acgttgccgt
      841 gcggccgggt cccccccacg gcggtcagac tgacggtctt gacccgccgg aagtagtgac
      901 ccgggaaatc gccgtcgagc agcccttcgg tgatctcgat ctggcaccgc ccggtcgcca
      961 gcagctcctc cagggccagc ggaacgtcct cgcgcagcga gacgttgcgc acgacctcca
     1021 gcgcgcggcg atcgacctcg agatacgcgc tctccatgcg gcgcaggtcg accagcaacc
     1081 gctcgcccgc gaacaacccc ttcttcaggc tgtcccagta cgagaactcg atgaacgacg
     1141 aggacggctc tccgcgctcg aagcggtacg cgcgctcggc ctgttgcgcc gcgtcgaacg
     1201 cgaccttgta ggcctggaag tagacggtgg acagctgacc cagcatccag ccatagagct
     1261 gttcgttcgt gtacttgtcg cgcaggtagc gctcgacctt gtgcgcgttc tcgacgtcga
     1321 cgtcgtgact gtgcagctcc gccgaggcga tctccagctt caggttcacc tcggcgatct
     1381 gcttgtcgag ctgggcctct tccttgagac gaagatcctt ttcgtgctgc cactcctcgc
     1441 gccgccgctg gtactcggcc tgcttctcgg ccatgtcggc ctcggtctcg ttcttggcca
     1501 tcaccttctg cacgctctcg gcgaacgcgc tggcgatgtc tccgagcatc ttgccgccca
     1561 gcttcaaggt cacgaacggc gagctgaacc cgcccgcggt gccggtctgg aaatccggaa
     1621 tggcatgcat caccttggcc acgaggttct ggccctccgc gatcgcggct tgcacctgcg
     1681 agaggctcag cgactgcagg ctggccgtct cctgcgggtt catcagctgc tgcagctggg
     1741 cgtcgtagta ctggatctcg atctcgagct gctcgcgctg gagcgacagc tggttcagct
     1801 gctcctcgac ctggcgcacc tgcttcttcc ggatgtcgcg caccgcctcg agcagcgccg
     1861 tctcgcccga ggcgcgcagc gccgccagcc cctcggcgtc gcggcgctcg agcaccgtcg
     1921 ccgtcatggc gccgaagctg cgcagctcct cggccagtcg cagcgcccgc gccagcaggt
     1981 agcggaaccg gtggtgcggt ggcggcgcgt tcaggctggc gatcacgctg ccgagatcga
     2041 cgcccgccgc cgccgcgcgc accagcaggc ccggatcgat cgggggctcg aacagcggga
     2101 gctgccgcac gatgccctgg atgttcatgc agttgcggat cttgaacagg cgatcggcga
     2161 cggtgtccca gtacttgtcc agctgcgggt tccccggcaa gcagaagtac tgcgtcacca
     2221 tgctcagcac ggacctcgcg ccgctgacgt cgggacgggc cgcgatccgg aacggccggc
     2281 gtacgcgctg gttcaggccc tgcaggctct cgaagcggat ctcgtagttg gcgaacaggt
     2341 ccagcttctt ccgcacctct ccgaacgtga gcggatcggg ctcgaccagg ggcgggatcc
     2401 gctcgggccg cggccccagg atgttgccgg ccaggatgta gagctgcgtg gcttcctgga
     2461 tggtctccag cgtgtcctgc cggaacagct tgtcgcccca ctcgatcagg ttgtcgatgt
     2521 acttcatcac cacggccttc tggtacgcga cggtgcgcag gcgcgcgatg acgtgcgggc
     2581 tgaacggctt ctcccaccag gccgtcacct gatcgatgac ccgctgctgg cggcggagcc
     2641 gaacggggtc gtcgccgctg tacgacagga gcgacatgat ctcgcgcgcg cccgcgtagt
     2701 cgtcgttctc gtggaacggc gcgaaccgcc agtagcgctg cggcggcggc gagctggcgt
     2761 cggtggtggg atcgaagatg aaatggaacc agcgctgcgc ttcttcgtgg cggccgtcct
     2821 tggccagacg aaccgccacc tgcagcggcg cgtggaagaa cagctcccag ttgtagagcg
     2881 agtacggccc gtcatgctcg aaatcgacgt cgaagtccgg ggggttgcgc gtgtcgacca
     2941 gcggtccggg cgcgtacgtc cgctcgaacc aggtcggtcg gagagggtgc cacttgccct
     3001 tgtcgtccag ccggtggtcg atggcctcct tgaatctcgg gggcgggcgc gtggtcgcga
     3061 ggtccagcag cccatcgatg ccggcgtccc gcagcttcat gatgaaccgg caggtcgccg
     3121 tgtgctcgaa cggcgagaac cgcagctgct gctcgtcata cttctcccgg cgcagcatga
     3181 cgaccggcgg ctgcgcgcgc accggc
//
LOCUS       DASVOR010001036         1613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_11063,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001036 DASVOR010000000
VERSION     DASVOR010001036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1613
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11063"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /locus_tag="VF516_19940"
     CDS             <1..941
                     /locus_tag="VF516_19940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110016.1"
                     /translation="PMEEPIEAPPGPVDPPWVAAVMAAAPPHPPEVQAVLDEQNLGDG
                     PKVARNFRMAYAATVTLHDAEGEAIHTIRYGRMPKGDVRGLCRGLARDVAAMRAQRPE
                     LKVTYLTDGASEFETLYEQHLKVPLGPDAMSLVDFWHAAEYLGAAARVFEIKRKAKPG
                     QFRRWRHALKHDDGAAAQIVEQLEQSGLEKVMLDGVRPVEAAIRYFKARLERMDYAGA
                     RGQGLPIGSGNVEATCKSLVTVRMKRPGARWKHTTGDEVLQLRALQLSDRWAPAVQRA
                     TRPLAKPVQIAVGVYAANTNRSAAPSTRGHGARTAA"
     gene            complement(1239..>1613)
                     /locus_tag="VF516_19945"
     CDS             complement(1239..>1613)
                     /locus_tag="VF516_19945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110017.1"
                     /translation="PELRRLRTPAGFRRARHILVTASDTGHGAHEAAQRSLEAVNAPD
                     TVLIRLPSFVAAAVVASRSDGVAIVPANLARALTGLGLATFRPPIAMPPIAVAQYWHE
                     RDQRDPGHRWLRAACFALFGRR"
BASE COUNT          261 a          553 c          604 g          195 t
ORIGIN      
        1 tgccgatgga ggaaccgatc gaggcgccgc cggggccggt ggatccaccg tgggtcgccg
       61 cggtgatggc tgcggcgccg ccgcatccgc ccgaggtcca ggccgtgctc gacgagcaga
      121 acctgggcga cgggcccaag gtcgcgcgca acttccgcat ggcgtacgcc gcgacggtga
      181 cgctgcacga cgccgagggc gaggcgatcc acacgatccg gtacggacgg atgcccaagg
      241 gtgacgtgcg tggactgtgt cgcgggctgg cgcgcgacgt cgctgcgatg cgcgcgcagc
      301 ggcccgagct caaggtcacg tacctgaccg acggcgcgag cgagtttgag acgctgtacg
      361 agcagcacct caaggtgccg ctcggtccgg atgccatgtc gctcgtcgac ttctggcacg
      421 ccgcggagta cctcggcgcc gcggcacggg tgttcgagat caagcgcaag gcgaagcctg
      481 ggcagttccg gcgctggcgg cacgccttga agcacgacga cggtgccgcg gcgcagatcg
      541 tcgagcaact cgagcagagc ggcctcgaga aggtcatgct cgacggcgtg cggcccgttg
      601 aagccgcgat ccggtacttc aaggcgcgcc ttgagcgcat ggactacgcg ggtgcgcggg
      661 gccagggctt gccgatcggc agcggcaacg tcgaggcgac gtgcaagagt ctggtgacag
      721 tccgcatgaa gcggcccggc gcgcggtgga agcacacgac cggcgacgag gtgctgcagc
      781 tgcgcgcgct gcagctgagc gaccgctggg cgccggccgt tcagcgcgcc accaggccgc
      841 tcgccaagcc cgtgcagatc gcggtcggcg tttacgccgc gaacacgaac cgcagcgccg
      901 cgccgagcac ccgcggccac ggagcgcgga ccgccgcgta gtcgacgcag aacctacggg
      961 cgggaggctc gaaacggctc cgacgaggat cggtgccgta aggtcggatt ctgcaacgaa
     1021 gtcacagtat tagaaccaca cccgatcgga gttgacataa taccgattct gcgacgcgac
     1081 cctgatcggt aagtgcccgc cgctcctggc cccgccaccg gccagcccgc gcctggccgt
     1141 gcacggcaca cgattccgcg gaatcgcgca cgcggcgttc ggactcgccg tggacggccc
     1201 ggcacggcac acgattccgc ggaatcgcgg gcccgcgatc aacgccggcc gaacagcgcg
     1261 aagcatgcgg cgcgcagcca gcggtggccg ggatcgcgct ggtcgcgctc gtgccagtac
     1321 tgggcgacgg cgatcggcgg catggcgatc ggcggccgga acgtcgcgag cccgagcccg
     1381 gtcagcgcac gcgccaggtt cgccgggacg atcgcgacgc catcgctgcg ggacgcgacc
     1441 accgcggcgg ccacgaagct cggaagccgg atcaggaccg tgtcgggcgc gttcacggcc
     1501 tccagcgagc gctgcgcggc ctcgtgggcg ccgtgcccgg tgtccgacgc ggtgaccagg
     1561 atgtggcgcg cgcggcgaaa cccggccggc gtgcgcagcc ggcggagctc ggg
//
LOCUS       DASVOR010001037         8130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_361221,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001037 DASVOR010000000
VERSION     DASVOR010001037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8130
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361221"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VF516_19950"
     CDS             <1..204
                     /locus_tag="VF516_19950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110018.1"
                     /translation="KLVEGAAKVLGDKAAIDALEEGEDHGLKDYRKDLDQLDARGRQL
                     VGGMIAMQQQTHDRLSALKRRMS"
     gene            complement(281..1084)
                     /locus_tag="VF516_19955"
     CDS             complement(281..1084)
                     /locus_tag="VF516_19955"
                     /inference="COORDINATES: protein motif:HMM:NF013676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HEX8110019.1"
                     /translation="MMYLRDMTAARARFVPVPTRPDRPGRPPVRRGLPQVPLTGCHAL
                     VSLVLRVPPRCCRRRADAHRLAWRALTTTRGQRRRSWSQSTDGLTVDVLHEMSKWFLR
                     TDTLKAANRVLVDYHHRLPLSSAWGDGTAVRDQRRQERGVGPLRPWLVAGQGDELACE
                     PAPSVDLDEQILDPLDLANVLGRLERIAAPELELAIADRGEGVGLEAGGKRLRRCIEL
                     GAQALHHLVGFQAPAWDRWADVVRGAIEACRLEAREPAGVRLGGGGDAV"
     gene            1143..2114
                     /locus_tag="VF516_19960"
     CDS             1143..2114
                     /locus_tag="VF516_19960"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8110020.1"
                     /translation="MSPDAAYHRAAADRKPEDPALVWTPDHVVLVATEIAPNVFAVCP
                     DDAARKNAAGIPAATSGGFVIGDQGVLIIDTMINRRLADQLVALIRERTTRPILYAVN
                     TSYHGDHSYGNQFLPAETKVIQHGNTQKYIQGHFPDDIAFMSQYFGTDSGLQELKPTR
                     AAILMKDGDARDFDLGGKIVTVKHLGFAQTSGDLFVWVAADRVLFTGNPIISGGSSFS
                     WLLDGHSGEALATLQRLRREFPDDAILVPGHGVPTGMAAIDAHIRYLTDLRREVSAAL
                     GDGLDPAAASERVSQRLAPSYGSYKIYPWVNTQLNVAKVYDELKVQR"
     gene            complement(2118..3005)
                     /locus_tag="VF516_19965"
     CDS             complement(2118..3005)
                     /locus_tag="VF516_19965"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8110021.1"
                     /translation="MLATCLASLLATSAARAEVAIDSRTARVDGMSLHYLAAGTGAET
                     VILIHGYAQSSRMWRATAIPELARRFTVIAPDLPGFGDSDIPPDGLDMKTAAVRIHDL
                     AKALHVARARVVGHDIGLMVAYAYAAMYPDEVSKLALMDAFLPGVGDWLAYYHNPALW
                     HFFFNGPTPEALVKGRERIYFEHFWNDFAGDPKHSLGEDDRRSYTAQYARPGRMHAGW
                     AYFAAFPQTAKDFAELAKTRLTMPVLVIAGGKAGGDFLVRQTQLVAANVTPVALAESG
                     HWLIDERPRETMDALARFL"
     gene            complement(3114..3887)
                     /locus_tag="VF516_19970"
     CDS             complement(3114..3887)
                     /locus_tag="VF516_19970"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8110022.1"
                     /translation="MTALSWEHWVERYQRQGFEVVARSWPGLEGDLAALRKDPSGLHD
                     LGITEIVEFYAGVIRGLDRKPIIMGHSFGALITQILLDEGFGVAGVSIDSAPVKGVLR
                     LPWSALKSAFPGLKNPANRHGAVTLTPEEFHYAFTNTMTDEESRAAYERYAVPGPGRP
                     LFQAAFANFNPHAASRIDFHNDNRAPLLVIAGGADHVSPSAINRTIAKLQHRSKSITA
                     YKEFAGRSHFILGQPGWEEVADFALAWALDPAELHDDAQ"
     gene            3897..4880
                     /locus_tag="VF516_19975"
     CDS             3897..4880
                     /locus_tag="VF516_19975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8110023.1"
                     /translation="MDQDDGRTRADMRVLDFRHVRSPCSTRAAAKRREHARRRLEPAL
                     HGATRAASGLVLNEQGDRMLKSLTLLVLGLTLAGAGCRDAAAEPEASEAEAAERSTPI
                     KNIVLVHGAFVDGGGWEAVYRRLKHDGYRVSVVQDGLTSLADDVATTRRVLDLQDGPT
                     ILVGHSYGGAVISESGNHAKVAGLVYVAAFALDSGESVQTLLENSPPGAPVPPILPPV
                     DGFLLLDRAKFHAAFAADLSAERAAFLADAQVPWAVAALAGTVGQTAWRSKPSWYLVT
                     TEDRMIAPDAQRFMARRAMATTVEVKGSHAIYESKPADVAALIEQAARLRR"
     gene            4905..6287
                     /locus_tag="VF516_19980"
     CDS             4905..6287
                     /locus_tag="VF516_19980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8110024.1"
                     /translation="MAGATDTRSEISNVRPVQERAMPHLYLEIECERPRAGPAWYRLA
                     GHSRVRIGRGDAREASLPEPGVLDVAVPDAWMSTRHAEIDLRPGGWALTDLGSKNGTL
                     RNGERVTVAELADGDRLQLGHTLFRFHAALRTGAAPAVLDARELASSLPALRTMSPRF
                     ARIAADVRAVAGSRAPVLICGESGTGKELVARAIHEMSGRRATFVAVNCGAIPRELIE
                     SELFGHRKGAFSGATADRLGFVRQSDGGTLFLDEIGDLPLPAQAALLRMLQESQATPV
                     GSDRPVDVDLRVVSATHRQLARMVREGTFRHDLLARLDGVRLELPALRDRDEDVPLLI
                     AGLLDRLAPDRPDLALAPDAAQALLEYHWPLNVRELEHALESALARAGGRPISRADLP
                     RAVTDTGGDAEPSTLSEADERQRSELLAKLGQHRGNLSAVARAMGKHRTQILRWMERY
                     GLDADRFRGG"
     gene            complement(6251..>8130)
                     /locus_tag="VF516_19985"
     CDS             complement(6251..>8130)
                     /locus_tag="VF516_19985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8110025.1"
                     /translation="EGRWGYALAYEKMPRQSFARRAADRSFVDVAVLADGRELAFGLE
                     PPFVIARDPRSGRELWRYRQPEPLTRLTVVPGGQVRLQLQSVDSYLDGATGRVLASFP
                     RGQPSPCSFAPVPSRVLFTVPMAGAWSLIFPQPSGEPVVLLPSMTGSGWPLCAASPEG
                     DRVAALDSAGVVHVWDLNRGVEVASRPAPNATELLFTAHGVAIVRGRTIQVFGAADGD
                     FFVALPSRGGTAVASPDGHRLVIARQTSNQADIVDLRARAVVSSLSYASGAARFAFSP
                     TSDRVVVAGILDGTVISAWDLYTPAASPPLPARHSVYRISQDGQRILVSAPLRIVDAR
                     GTVVRTLDLGGAAMLNANLSTDGRRAAVTTVDHLSVFDTETGAEISRIEVGDSKRVGI
                     SRDGARVLTGNTQGITVWDVDRRQVIWREARPRPVGGPLALAPDGQGVLWGGEGVVHY
                     RTVDGQVRELALGENVFDAEFSYDSARLAVVTSRSIGVWELDGLRPVFRISNPASVYQ
                     EIYWSADDATLFDLREFVNTVLLDSRTGHRLATIEPSKPEANSAHDRPSPDLRHRIAR
                     GDDAFDLRPLPSPDQQAPDASLARVLDEAGLELRGVELDYVAPPLEPPQPPRNRSASR
                     P"
BASE COUNT         1246 a         2873 c         2891 g         1120 t
ORIGIN      
        1 aagctcgtcg agggcgcggc gaaggtgctg ggcgacaagg cggcgatcga tgccctcgag
       61 gaaggcgagg accacggcct gaaggactac cggaaggacc tcgaccagct cgacgcccgg
      121 ggccggcagc tcgtcggagg gatgatcgcg atgcagcagc agacccacga ccggctgagc
      181 gcgctgaagc gccgcatgtc ctagcctgct gcatcgccga gcaccgcggc gctgggcacc
      241 tcgcgtctcg aggatcacaa gccgatgcta cctcctcggc tcatacagca tcgccaccgc
      301 ccccgagccg aactccagcc ggctcacgag cttcaagtcg acatgcttcg atagccccgc
      361 gaacaacgtc ggcccacctg tcccacgcgg gagcctggaa gccgaccagg tggtggagcg
      421 cctgcgcacc gagctcgatg cagcggcgca gtcgcttgcc accggcctcg aggccaaccc
      481 cttcgccgcg atccgcgatg gccagctcga gctccggcgc cgcgatgcgc tcgagccgcc
      541 cgagcacgtt cgcgagatca agcgggtcga ggatctgctc gtcgaggtcg accgatggtg
      601 cgggttcaca cgcgagctcg tccccttgcc cggctaccag ccacggacgg agcggcccta
      661 caccgcgctc ctggcggcgc tggtcgcgca cggctgtgcc gtcgccccag gccgacgaca
      721 gcggcaggcg atgatgatag tcgacgagca cgcggttggc tgccttgagc gtgtccgtgc
      781 ggaggaacca cttggacatc tcgtgcagca cgtcgacggt caggccgtcg gtgctctgcg
      841 accacgagcg acgacgctgc ccgcgtgtcg tagtgagcgc gcgccaagca aggcggtgcg
      901 cgtccgctcg ccgccggcag caccggggcg ggacgcgcag aacgagtgac accagcgcgt
      961 gacagccggt gagtggcacc tgtggcaagc cgcgccgcac aggcggtcgt ccgggccgat
     1021 cggggcgcgt cgggaccggc acgaatctgg ctcgtgccgc agtcatgtcc ctcaaataca
     1081 tcatctttgc gtcagggctg gcgatcgtgg gttgcgccgg cgctggaccg aactcgaccc
     1141 tcgtgtcacc cgacgccgcg taccaccggg ccgcggcgga tcgaaagccg gaagaccccg
     1201 cgctggtgtg gacgcccgat cacgtggtgc tggtcgccac cgagatcgcg cccaacgtgt
     1261 tcgcggtgtg cccggacgat gccgcccgca agaacgcggc cggcatcccg gcggcgacct
     1321 ccggcgggtt cgtgatcggc gatcagggcg tcctgatcat cgacaccatg atcaatcgcc
     1381 gcctggccga tcagctggtc gcgctgatcc gcgagcggac cacccggccg atcctctacg
     1441 cggtcaacac gagctatcac ggcgatcatt cgtacggcaa tcagttcctc cccgcggaga
     1501 ccaaggtcat ccagcacgga aacacgcaga agtacataca gggccacttc ccggacgaca
     1561 tcgcgttcat gtcgcagtac ttcggtaccg actcgggtct gcaggagttg aagccgaccc
     1621 gagcggcgat cctgatgaag gacggtgacg cccgcgactt cgacctcggg ggcaagatcg
     1681 tcacggtgaa gcacctgggc ttcgcccaga ccagcggcga cctgttcgtc tgggtcgcag
     1741 cggaccgcgt gctgttcacc ggcaacccga tcatcagcgg cgggtcctcg ttctcgtggc
     1801 tactggacgg ccacagtggc gaggccctgg ccacgttgca gcggctgcgc cgcgagttcc
     1861 cggacgacgc gatcctcgta cccgggcacg gcgtgccgac cggcatggcg gcgatcgacg
     1921 cccacatccg ctacctgacc gacctccggc gcgaggtctc ggcggcgctg ggcgacggcc
     1981 tcgacccggc cgcggcctcc gagcgggtga gccagcgcct cgcgccgagc tacggcagct
     2041 acaagatcta tccgtgggtg aacacgcagc tcaacgtcgc caaggtctac gacgagctca
     2101 aggtccagcg ctgaggctca caggaaccgc gcgagcgcgt ccatggtctc gcgcggccgc
     2161 tcgtcgatca gccagtgccc ggactccgcg agcgccaccg gcgtgacgtt cgcagcgacg
     2221 agctgggtct gccggaccag gaagtcgccg cccgccttgc cgccggcgat cacgagcacg
     2281 ggcatcgtga gcctggtctt cgccagctcg gcgaagtcct tcgcggtctg cgggaacgcg
     2341 gcgaagtacg cccagccggc gtgcatccgg cccgggcgcg cgtactgggc cgtgtaggac
     2401 cggcggtcgt cctcgccgag cgagtgcttc ggatcaccgg cgaagtcgtt ccagaagtgc
     2461 tcgaagtaga tccgctcgcg acccttcacg agcgcctccg gcgtcggccc gttgaagaag
     2521 aagtgccaga gcgccgggtt gtgatagtag gccagccagt cgcccacgcc cggcaggaac
     2581 gcgtccatga gggcgagctt ggagacctcg tccgggtaca tcgccgcgta ggcgtaggcc
     2641 accatcaggc cgatgtcgtg gccgacgacg cgcgcgcggg ccacgtggag cgctttggcg
     2701 aggtcgtgga tgcggaccgc cgcggtcttc atgtcgaggc cgtcgggcgg gatgtcggag
     2761 tcgccgaacc ccggcaggtc aggcgcgatc accgtgaatc gcctggcgag ctccgggatc
     2821 gcggtcgccc gccacatccg agacgactgg gcgtagccgt ggatcaggat caccgtctcg
     2881 gccccggtcc cggcggccag gtaatggagg ctcatgccat cgacccgggc ggtcctggag
     2941 tcgatcgcga cctccgcccg ggcagcggag gtggccagga gcgacgcgag gcaggtggcg
     3001 agcaccaccg agagcgcgcg aacggatggt cgcagggatc gtcgtactgg agttgtcgtc
     3061 atgcctcgcc gtcgagcaca tcgcgtgcca ctcgacccgc ggcggcgcga cggtcactgc
     3121 gcgtcgtcgt gcagctcggc cggatcgagc gcccacgcca gcgcgaagtc ggcgacctcc
     3181 tcccagccgg gctggccgag gatgaaatgg ctgcggccgg cgaactcctt gtaggccgtg
     3241 atcgacttcg agcggtgctg cagcttcgcg atcgtgcggt tgatcgccga cggcgacaca
     3301 tggtcggctc cgccggcgat cacgagcagc ggcgcgcggt tgtcgttgtg gaagtcgatc
     3361 cggctggcgg cgtgcgggtt gaagttcgcg aacgcggcct gaaacagcgg gcggcctggc
     3421 cccggcacgg cgtagcgctc gtaggcggcg cgcgactcct cgtcggtcat cgtgttggtg
     3481 aacgcgtagt ggaactcctc gggagtcaac gtcaccgcgc cgtgccggtt cgccgggttc
     3541 ttcaatcccg gaaacgccga cttgagcgcc gaccacggca gccgcagcac gcccttgacc
     3601 ggcgccgagt cgatcgagac tcccgcgacg ccgaagccct cgtcgagcag gatctgcgtg
     3661 atcagcgcac cgaacgagtg gcccatgatg atcggcttgc gatccagccc gcggatcaca
     3721 ccggcgtaga actcgacgat ctcggtgatg ccgaggtcgt ggaggcccga cgggtccttg
     3781 cgcagcgcag cgaggtcgcc ctcgagaccg ggccagctcc gcgcgacgac ctcgaagccc
     3841 tggcgctggt agcgctccac ccagtgctcc caggagagcg cggtcatcca caggccgtgg
     3901 atcaggacga tgggaggacg cgagccgaca tgcgagtttt ggatttccgt catgtgcgct
     3961 cgccgtgcag cacgcgtgcc gccgcgaaac gccgggagca cgcgcgacgg agactggagc
     4021 cagcgctgca tggcgcgacg cgtgcagcga gcggcctcgt tctcaacgaa caaggagacc
     4081 gcatgttgaa atcgctaaca ctcttggtgc tcggactgac ccttgccggt gcgggttgtc
     4141 gtgatgcagc ggccgagccg gaggcttccg aggcggaggc tgccgagcgc tccacaccga
     4201 tcaagaacat cgtgctggtg cacggagcgt tcgtggacgg tgggggctgg gaagccgtct
     4261 acaggcgcct caagcacgac ggctaccgcg tcagcgtggt ccaggacggc ctgacctcgc
     4321 tcgccgacga cgtcgcgacg accaggcgcg tcctcgatct ccaggacggg ccaacgatcc
     4381 tcgtcggtca ctcctacggc ggagccgtga tcagcgagtc gggcaaccac gcgaaggtgg
     4441 ctggcctggt ctacgtggcc gcgtttgcgc tcgacagcgg cgaatcggtc cagaccctgc
     4501 tcgagaactc gccgcccggg gctcccgtgc ccccgatcct gccgccggtc gacggcttcc
     4561 tgttgctgga cagggcgaag ttccacgccg cgttcgccgc cgacctgagc gccgaacgcg
     4621 ctgccttcct ggcggatgcc caggtcccgt gggcggtcgc cgcgctggcc ggaaccgtcg
     4681 ggcagaccgc gtggcggtcc aagccgagct ggtacctggt cacgacggag gaccggatga
     4741 tcgcgccgga cgcacagcgg ttcatggcca ggcgcgcgat ggcgaccacg gtcgaggtca
     4801 agggaagtca cgcgatctac gagtcgaaac cggcggatgt cgccgcgctc atcgagcagg
     4861 cggcgcggct gcgcaggtag ccgtgcggac cgtataatgc aggcgtggcc ggcgcgaccg
     4921 acaccaggtc cgagatctcg aacgtgaggc cggtgcaaga gcgggcgatg ccgcacctct
     4981 acctcgagat cgagtgcgag cggccgcgcg cgggcccggc atggtatcgg ctggcgggcc
     5041 acagccgcgt gcgcatcggc cggggggacg cgcgcgaggc gtcgctgccc gagccaggcg
     5101 tcctcgatgt cgcggtcccc gatgcgtgga tgtcgacccg tcacgccgag atcgacctgc
     5161 gcccgggcgg ctgggcgctg accgacctcg gctcgaagaa cggtacgctg cgcaacggcg
     5221 agcgcgtcac cgtcgccgag ctcgccgacg gcgaccggct gcagctcggg cacacgctgt
     5281 tccggttcca cgccgcgctg cggacgggag cagctcccgc ggtgctcgat gcgcgcgagc
     5341 tcgcgagctc gctgccggcg ctgcgcacga tgtcaccgag gttcgcccgg atcgcggccg
     5401 acgtgcgtgc cgtggccggg tcgcgcgcgc ccgtgctgat ctgcggcgag agcggcacgg
     5461 gcaaggagct cgtggcacga gccatccacg agatgtccgg gcggcgcgcg acgttcgtcg
     5521 cggtcaactg cggcgcgatc cccagggagc tgatcgagtc cgagctgttt ggccaccgca
     5581 agggcgcatt ctccggcgct accgccgacc ggctcggctt cgtgcgccag tcggacggcg
     5641 gcacgctgtt tctcgacgag atcggcgacc tgccgctgcc cgcgcaggcg gcgctgctgc
     5701 gcatgctcca ggagtcccag gcgacgccgg tcggcagcga ccggccggtc gacgtcgacc
     5761 tgcgggtcgt gagcgcgacc caccgccagc tcgcgcgcat ggtgcgcgag ggcacgttcc
     5821 gccacgatct cctggcgcgg ctcgacggcg tgcggctcga gcttcccgcg ctgcgcgaca
     5881 gggacgagga cgtgccgctg ctcatcgccg gcctgctcga tcggctcgcg cccgaccggc
     5941 ccgatctcgc gctcgcgccc gacgccgcgc aggcgctgct cgagtaccac tggccgctca
     6001 acgtgcgcga gctcgagcac gcgctcgaga gcgccctcgc gcgcgccggc gggcggccga
     6061 tatcgcgagc ggacttgcca cgggccgtca ccgacacggg cggcgatgcc gagccgtcga
     6121 ccctgtccga ggccgacgag cgccagcgct cggagctgct cgcgaagctc gggcagcacc
     6181 ggggcaacct gtccgcggtg gcgcgcgcga tgggcaagca ccggacccag atcctgcgct
     6241 ggatggagcg ctacggcctc gacgccgacc ggttccgcgg cggctgaggc ggttcgagcg
     6301 gcggcgccac gtagtcaagc tcgacgccgc gcagctccag acccgcttcg tcgaggaccc
     6361 gcgccaggct cgcgtcgggg gcttgctggt ccggcgaggg cagggggcgg aggtcgaacg
     6421 catcatcgcc ccgggcgatc cggtgccgga ggtccggcga gggccggtcg tgggcggagt
     6481 tcgcctccgg cttcgacggc tcgatggtcg ccaggcgatg cccggtgcga ctgtcgagca
     6541 gcaccgtgtt cacgaactcc cggaggtcga acagcgttgc gtcgtccgcc gaccagtaga
     6601 tctcctgata gaccgacgct ggattcgaga tccggaacac cggccgcagg ccgtcgagct
     6661 cccagacacc gatcgaccgg ctggtgacca cggccagtcg tgcgctgtcg tagctgaact
     6721 ccgcgtcgaa cacgttctcg cccagcgcga gctcgcggac ctgcccgtcg acggtgcggt
     6781 agtgcacgac gccctcgccg ccccacagca cgccctggcc atccggggcc agggccagcg
     6841 gtccgccgac cggacgcggc cgcgcctcgc gccagatcac ctgccgccga tcgacgtccc
     6901 acacggtgat cccctgggtg ttgccggtca gcacgcgagc tccgtcgcgc gagatcccga
     6961 cccgcttgct gtcgccgacc tcgatgcgcg agatctccgc gccggtctcg gtgtcgaaca
     7021 cgctcaggtg atccaccgtc gtcacggcgg cccggcggcc gtccgtcgag aggttcgcgt
     7081 tcagcatcgc ggcgcccccc aggtcgagcg tccgcaccac cgttccgcgg gcgtccacga
     7141 tccgcagcgg ggcgctgacc aggatgcgct gaccgtcctg cgagatccgg tagacgctgt
     7201 gacgggcggg caggggcggc gacgccgcgg gcgtgtagag gtcccacgcc gagatcacgg
     7261 tgccgtcgag gatgccggcg accaccaccc ggtcgctggt gggcgagaac gcgaagcgcg
     7321 ccgcgccgct cgcatagctc agcgagctga ccacggcccg cgcacgcagg tcgacgatgt
     7381 ccgcctggtt gctggtctgg cgcgcgatca ccagccggtg accgtcgggc gacgcgacgg
     7441 cggtgccgcc gcggctgggc aacgcgacga agaagtcgcc gtcggccgcg ccgaacacct
     7501 ggatcgtacg accgcggacg atggcgacgc cgtgggccgt gaacagcagc tcggtcgcgt
     7561 tcggtgccgg gcggctggcg acctccacgc cccggttcag gtcccagaca tgaacgacgc
     7621 ccgcgctgtc cagggccgcc acccggtcac cctccggcga cgccgcgcac agcggccacc
     7681 cggacccggt catggacggc aacaggacga cgggctcgcc tgacggctgg gggaagatca
     7741 ggctccaggc gccggccatg ggtacagtga acagcacgcg cgacggcacg ggcgcgaacg
     7801 agcacggact gggctggccg cgggggaagc tcgccagcac gcgcccggtg gcgccgtcga
     7861 ggtagctgtc caccgactgc agctgcaacc ggacctggcc gccggggacc acggtgagcc
     7921 gggtgagcgg ttcgggttgc cggtaccgcc acagctcgcg cccgctccgc ggatctcgcg
     7981 cgatcacgaa cggcggctcc agcccgaacg ccagctcgcg cccgtcggcc aggaccgcca
     8041 cgtcgacgaa cgagcggtcc gccgcccggc gcgcgaacga ctggcgcggc atcttctcgt
     8101 acgccagcgc gtacccccag cggccctccg
//
LOCUS       DASVOR010001038         5742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_418910,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001038 DASVOR010000000
VERSION     DASVOR010001038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5742
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418910"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VF516_19990"
     CDS             <1..991
                     /locus_tag="VF516_19990"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8110026.1"
                     /translation="DALDASPAPTPQGTVLGSVPALYAHAQRERVPLFAMCECTYRCN
                     LRCTHCFILHKVSAHRPDLVPTEHMLRLLDELAELGCLEVTLTGGETTLHPDYRALVS
                     AARSLHLVTTLKTNATTFTRERACEYAADPAHATEVSLYGATPEVHDAFTATPGSFAK
                     TLRGLVELGRAGVRCTVFSIIWERNAAQVDDIARLVEDLGHRSVFNDVIYGRLDGDHA
                     PTALRATRDARDGLIAQGRLKPFDPMPCIAGRVKVKIAADGAIASCELFPAGFGNVWS
                     SGFGALWRDRGFVGEADRIVQLSTAHRKDGRPVLTCPAMNQLNTGRMEGLTTV"
     gene            complement(1109..1891)
                     /locus_tag="VF516_19995"
     CDS             complement(1109..1891)
                     /locus_tag="VF516_19995"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8110027.1"
                     /translation="MSIPPNGPGKVAVVIGGTSGIGLATARLLVDMGARVTITGRDAE
                     RGARASQELGERAHYARMDVARADQVAAAIDDTVQRWGRLDWAVNAAALSDMPFARIA
                     DITEEDWDRTLAVDLKGVWLAMKYELAAMIGTGGGSIVNVSSINGLSATPQAGAYCVA
                     KHGLHGLTKTAAAEYAAQGIRINVVCPGAHLTPMLEVVFDRVAPGEPDKAAAMYRARI
                     PMNRIGDPAECARAIVWLLSDAASYVTGAVLTVDGGLALGTA"
     gene            complement(1888..2085)
                     /locus_tag="VF516_20000"
     CDS             complement(1888..2085)
                     /locus_tag="VF516_20000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110028.1"
                     /translation="MDTSETLPGDQLVVPSARHVSAKARSFVDHAARALATLGVIRAL
                     PARRDHRAARSHRAALLGSRP"
     gene            complement(2136..3095)
                     /locus_tag="VF516_20005"
     CDS             complement(2136..3095)
                     /locus_tag="VF516_20005"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8110029.1"
                     /translation="MTHELDLEDIMHTQSSASPILVTGVPGNIGGLGRAIVEDLRRRG
                     LPVRALAHREDERTDALRSLGAEIVTGDLTHPEDVSRAFDGCRRVCFGMGVSASYLEA
                     TVIAAAVARQHKDLEVLVNISQMTVSQMSLTEMTGSPQHQQQWLGEQVLNWSGAPVVH
                     VRPTVFLQHPFFTMLAAESIARSGTVRLPFGAARTSPIDAQDVADVVTEILANPKPHV
                     GKVYELTGPRSEDMTAIAAEYSEALGRKISYVDVPFAAWRDELRAHNLPEHVYHHFVV
                     MAQLHAEDRYDRLTHDVDALLGRPATSVRAYAAKHPESWRAQP"
     gene            3694..4122
                     /locus_tag="VF516_20010"
     CDS             3694..4122
                     /locus_tag="VF516_20010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110030.1"
                     /translation="MPATRSPSWPRRPEVLRPRGLERRRSPPRRAHGLRARNDRPVGG
                     DRGIQLDGLRAEGLHPQPWLPRSIATTLCVHESSVLSRLRKRLAETFEQPIDHLFFRE
                     RYLPGEDPNAALLRMFKRLQWMVSLTDEQTIDSMARRSHR"
     gene            4119..4526
                     /locus_tag="VF516_20015"
     CDS             4119..4526
                     /locus_tag="VF516_20015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110031.1"
                     /translation="MTRVILYRVEAKPQVISVSPSAAGGYSTATEELLGGPVACVPLH
                     DGIQLYCNRGGLLFGLALARRALALSLAEPSRFEVRLRFENSELDLGPKEWPASGDFV
                     LARVTNGGELVDLSEDDIRLWMLWLGLDYILNR"
     gene            4983..>5742
                     /locus_tag="VF516_20020"
     CDS             4983..>5742
                     /locus_tag="VF516_20020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110032.1"
                     /translation="MCSEQLTNAPCYFQNHPSCVRNDTCAGSFFTCNAACPAPTAADI
                     NCGPPPPYQCHEPNGSVPLPLLEHYHVRIDPDSLYVCDTLLGWEAHFPWPQLSWGEIN
                     EECVGACGAGCSSSTCTRTGSGDYVDIGDGQACRDTHYDCYAADCCWYHDLCGRMFPT
                     AVFTDPFCHALGIVYGCAACLGSGFPGCALGPSYTVGFDHPYTDREDCVCADRPGDCV
                     DDCSGACWAGCDDNSDCFDDCTGEYVCGDPFCPDF"
BASE COUNT          854 a         2038 c         1943 g          907 t
ORIGIN      
        1 cgacgccctc gacgcctcgc cggcgcccac cccgcagggg acggtgctcg gcagcgtccc
       61 ggcgctgtac gcgcacgccc agcgcgagcg ggtccccctg ttcgcgatgt gcgagtgcac
      121 gtaccgctgc aacctgcgct gcacgcactg cttcatcctc cacaaggtct cggcgcaccg
      181 cccggacctc gtgcccaccg agcacatgct ccgcctgctc gacgagctgg ccgagctcgg
      241 ctgcctcgag gtcacgctga ccggcggtga gaccaccctg caccccgact atcgcgcgct
      301 ggtctcggcc gcgcggtcgc tgcacctggt caccacgctc aagaccaacg ccaccacctt
      361 cacccgcgag cgcgcctgcg agtacgccgc agatcctgcg cacgccacgg aggtcagcct
      421 ctacggcgcc acgcccgagg tccatgacgc gttcaccgcc acgcccggca gcttcgcgaa
      481 gaccctgcgc ggcctggtcg agctcgggcg cgccggcgtg cgctgcacgg tgttcagcat
      541 catctgggaa cgcaacgccg cgcaggtcga cgacatcgcg cggctcgtcg aggatctcgg
      601 ccaccggagc gtgttcaacg acgtcatcta cggccgcctc gacggcgacc acgccccgac
      661 cgcgctgcgc gcgacccgcg acgcgcgcga cggcctgatc gcgcagggtc ggctcaagcc
      721 gttcgatccg atgccgtgta tcgccggccg ggtcaaggtc aagatcgccg ccgacggggc
      781 catcgccagc tgcgagctgt tccccgcggg tttcggcaac gtgtggtcct cggggttcgg
      841 cgcgctgtgg cgcgaccgtg gcttcgtggg cgaggccgat cgcatcgtgc agctgtcgac
      901 tgctcaccgc aaggacggcc ggccggtcct gacgtgcccg gcgatgaacc agctcaacac
      961 cggtcggatg gaagggctca ccaccgtcta gggcgggctt gcagcgagat cctccagtgg
     1021 cagatcgtgg atgacgatct ctggctcgcg gtgcacgagc ggttcacctc gcgcggcccg
     1081 cgtgctccgg aggcccgccc gttgacgctc aggcggtgcc cagcgcgagg ccgccatcga
     1141 cggtcagcac ggcgccggtc acgtacgacg cggcatcgga cagcagccac acgatcgccc
     1201 gcgcgcactc ggccgggtcg ccgatccggt tcatcgggat ccgggcgcgg tacatcgcgg
     1261 ccgccttgtc gggctctccc ggcgccaccc tgtcgaacac gacctcgagc atcggcgtga
     1321 ggtgcgcgcc gggacacacg acgttgatcc ggatgccctg cgcggcgtac tcggccgccg
     1381 cggtcttggt caggccgtgc agtccgtgct tggcgacgca gtacgcgccg gcctgcggcg
     1441 tcgcggacag gccgttgatg gacgacacgt tgacgatcga tccgccgccg gtcccgatca
     1501 tcgccgcgag ctcgtacttc atcgccagcc acacgccctt gagatcgacc gcgagggtcc
     1561 ggtcccaatc ctcttcggtg atgtcggcga tgcgcgcgaa cggcatgtct gacagggcgg
     1621 cggcgttgac cgcccagtcg agccggcccc agcgctgcac ggtgtcgtcg atggccgccg
     1681 cgacctggtc ggcgcgcgcg acgtccatgc gcgcgtagtg cgcgcgctcc cccagctcct
     1741 ggctcgcgcg cgccccgcgc tcggcgtcgc ggccggtgat cgtgacgcgc gcgcccatgt
     1801 cgaccaggag ccgcgcggtc gccaggccga tgccggacgt acctccgatc acgacagcga
     1861 ccttgccggg accattcggt gggatgctca tggccgcgaa cctagcaggg cggcgcgatg
     1921 gctacgagcc gcccggtggt cacggcgcgc cggcagcgcg cgaatcacgc ccagtgtcgc
     1981 gagcgcgcgg gcggcgtgat cgacgaagct gcgcgccttt gccgaaacat gccgtgcgct
     2041 cggcacgacg agctggtctc ccgggagtgt ctcgctggtg tccatcgatc agacccttcg
     2101 cattgccgac gcccaccccc ttcgggagga cggcatcagg gctgggctct ccacgactcg
     2161 ggatgtttcg cggcgtacgc gcggacgctg gtcgcaggtc tgcccaggag cgcatcgacg
     2221 tcatgcgtga ggcggtcgta gcgatcctcg gcgtgcagct gcgccatcac cacgaagtgg
     2281 tgatagacgt gctcgggaag gttgtgagct cggagctcgt cgcgccaggc ggcgaatggc
     2341 acgtcgacgt acgagatctt gcggccgagc gcctcggagt attcggccgc gatggccgtc
     2401 atatcttccg agcgcggccc ggtgagctcg tagaccttgc cgacgtgagg cttcgggttc
     2461 gcgagaatct ccgtgacgac gtctgcgacg tcctgggcat cgatcgggga ggtgcgtgcg
     2521 gcgccgaacg ggaggcggac cgtgccggag cgcgcgatcg actctgccgc gagcatcgtg
     2581 aagaacgggt gctggagaaa cacggtcggg cggacgtgaa cgaccggagc ccccgaccag
     2641 ttgagcacct gttcgccgag ccactgctgc tggtgctgcg gggaccccgt catctcggtg
     2701 agcgacatct gcgagaccgt catctgcgag atattgacga gcacctcgag atctttgtgc
     2761 tgccgcgcca cggcggcagc gatgacggtg gcttcgaggt acgacgcgga gacgcccata
     2821 ccgaagcaga cgcgacggca gccgtcgaac gcgcggctca cgtcttcggg gtgtgtgagg
     2881 tcacccgtca cgatctccgc gcccagggag cgcagagcat ccgtacgctc gtcctcgcgg
     2941 tgcgcgagcg cgcgcacggg aaggccgcgg cggcgaaggt cctcgacgat ggcgcgaccg
     3001 aggcctccga tgttgccggg taccccggtc acgaggatgg gtgaagcgct ggattgcgtg
     3061 tgcatgatgt cctcaaggtc gagttcatgg gtcactccgt ccgccgttcg ctgccgacga
     3121 acacaaccat gcgccgccga gcgccggctc ggtagagtgg attgttgcga atcatcttgt
     3181 atcggatgca aagttctctg gcaccgcgca tcgtactcca gcagatgatc gccttcgccg
     3241 gatcgccgag gccggcagca tctccaggcg gcgcgctcgc tcgggcttcg gatcgagaag
     3301 ggcgtcggct acacccagaa cccccatgtc tcagggaagt tgtcgcctga gccggcatga
     3361 ctgaccggcg tcagacccaa ccgatagaca aggggcacga ggaccccgtg gctgagtact
     3421 ctgagagctt tcggaaccga atggtcgagc tcaacggcgc ctttcagcgc ggccgggggg
     3481 gcccggcggc tgctggatcc tgttcgagcc cgagctccag ctgaccgcgc tggagccgat
     3541 ggcacccgac atcgccggct ggcgcgtgga gcgcatgccc gcgttgcacg ggccagctcg
     3601 ccggtccgca tccaccgttc atcctgccgg cgccgctcgc cgtgccgccg ctcgccggcc
     3661 gcgaggccga actggaccgc atcatcgcgg agtatgccgg cgacgcgatc gccgagctgg
     3721 ccgcgtcgcc ctgaagtcct tcgaccgcgc ggactggagc ggcgccgatc tcctcctcgt
     3781 cgagcgcacg gcctacgtgc tcggaatgat cgcccggtcg gcggcgaccg cggcatccag
     3841 ctcgacgggc tacgcgccga gggcctgcac ccgcagccct ggctcccgcg ctcgatcgcc
     3901 accaccctgt gcgtccacga gtcctcggtc ctcagccgtc tgcgcaagcg gcttgcggag
     3961 acgttcgagc agccgatcga ccacctattc ttccgcgagc gctacctccc cggcgaggat
     4021 cccaacgccg cgctgctgcg gatgttcaag cggcttcagt ggatggtgag cttgaccgac
     4081 gagcagacca tcgactcgat ggcgcgccgg agccaccgat gacccgggtc atcctgtacc
     4141 gggtggaggc aaagccccag gtgatctccg tctcgccgag cgccgccgga ggctactcga
     4201 cggccacgga ggagctgctg ggcgggccgg tcgcgtgcgt tccgctgcac gacggtatcc
     4261 agctctactg caaccgcggc gggctgctgt tcggcctcgc gctcgctcgt cgcgcgctgg
     4321 cgctgtccct cgccgagccg tcgcggttcg aggtgaggct ccgcttcgag aactcggagc
     4381 tcgacctcgg cccgaaggag tggcctgcca gcggcgactt cgtgctggcc cgggtcacca
     4441 acggcggcga gctggtggac ctgagcgagg acgacatcag gctctggatg ttgtggctcg
     4501 ggctcgacta catcctgaat cgctgagcag ccgccaggcc tcgcggccga ccgcggcggc
     4561 gctcgacctc agcgctcggc ccggaggtca cccgctcggc ggcggtacag aattccgtcg
     4621 tgaacaatga cctcaggctc agcgacaccg gcgccatccc gagccgcgcc gccagtgatc
     4681 gagacacgca tgcacgcctt gcccgaccgc gggcgcatac ggtatgtgaa tagcgttctc
     4741 aaaccctgag aggtctcatg atccgtttcg tcatcatcgc agttgccgtg ctgggggcgt
     4801 gttctaccct cgagaggcgc gacgaccagc gagacaccga tcggctgcaa tcgcctgccg
     4861 gatcgcagct ggacgatcag ccgccctttg taccgatcga tccaccaccg gacgatccgg
     4921 gcctgagcac ctgcgacggg acccccgggc aatggaacgg gtgccgcggc aacggttgcg
     4981 ccgtgtgctc cgagcagctc accaacgcgc cgtgttactt ccagaaccat ccgagttgcg
     5041 tccgcaacga cacctgcgcc ggcagcttct tcacctgcaa cgccgcctgc cccgcgccga
     5101 cggccgccga catcaactgc ggccccccgc ccccctacca gtgccacgag ccgaacggat
     5161 ccgtgccgct gcccctgctc gagcattacc atgtgcggat cgaccccgac tcgctgtacg
     5221 tgtgcgacac cctgctcggc tgggaggcgc acttcccgtg gccgcagctg tcgtggggcg
     5281 agatcaacga ggagtgtgtc ggcgcgtgcg gcgccggctg cagctccagc acgtgcacgc
     5341 gcacggggtc cggcgattac gtcgacatag gcgacggaca ggcgtgccgc gatacgcact
     5401 acgactgcta cgcggccgac tgctgctggt accacgatct gtgcggccgg atgttcccga
     5461 ccgcggtgtt caccgatccg ttctgccatg cgctcggaat cgtctacggt tgcgcggcgt
     5521 gcctcggctc cgggttcccg ggctgcgcgc tcgggcccag ctacaccgtg ggtttcgatc
     5581 acccgtacac cgatcgcgag gattgcgtct gcgccgaccg gccgggcgac tgcgtcgacg
     5641 actgcagcgg tgcatgctgg gcgggttgtg acgacaactc ggattgcttc gacgactgca
     5701 ccggggagta cgtctgcggc gatccgttct gtccggactt ct
//
LOCUS       DASVOR010001039         4300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_215151,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001039 DASVOR010000000
VERSION     DASVOR010001039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215151"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VF516_20025"
     CDS             <1..448
                     /locus_tag="VF516_20025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110033.1"
                     /translation="NTGIRGLDVASIQHAMLDGVDRTRARLGAAGQLDRLDLAVELVG
                     APPALPRKTLLIGRWRPDRFDAWAGEIARRGVTRTRSIVLPAALRGTDLDIYVYHLGG
                     PAGGVARRLGTARDPTLGFLPWSRGGYWALACNPDECFVIAATRVL"
     gene            496..1494
                     /locus_tag="VF516_20030"
     CDS             496..1494
                     /locus_tag="VF516_20030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8110034.1"
                     /translation="MDPVVSIIAPAYNEAKNLAAFLAAITPVLDGLGEPWEIVFVDDG
                     SRDDTLGMLLAARAQDSRIKVVGLARNFGKDVALSAGLAHARGRAVIPIDCDLQHPPA
                     LIPELVAKWRAGADMVIAVRTRRSEEGVVRRTAAGAYYAVMRRMTQVEIPRNAGDFRL
                     LDRKIVDIINQMPERCRFMKGIFAWPGFKTESVGFQAAVRAGGRSSWSLWKLWRFALD
                     GLFSFSTVPLKLWTYLGALFALGAFIYLAITLVQKLVFGIDAPGYASLLIVMLGFNGL
                     LLLSNGIQGEYIARIFEEVKGRPLFVVAGTWGFDEPAVRVAPAATAEPAASRDAVS"
     gene            1563..2714
                     /gene="add"
                     /locus_tag="VF516_20035"
     CDS             1563..2714
                     /gene="add"
                     /locus_tag="VF516_20035"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX8110035.1"
                     /translation="MTTGEITYDLLQRLPKTDLHCHLDGSLRLDTVIDLARHQGVKLP
                     TFDRGELHRMLAAGEQVASLDDYLRAFDITLSVMQVEDALERAAYELAEDAWRENVRY
                     LEVRYSPLLHTRQGLRPAQVVEAVLRGLRMAKRELGIRYGVILCAIRSLGPESSLRIA
                     ELCIAFKNRGVVGFDLAGSESGNPAKVHKHAFQLVVDNNINCTAHAGESFGPDSVHQA
                     IHKCGAHRIGHGTRLVESGDLLNYVNDHRIPLEVCPSSNVQTHAATGWDTHPVDFFVD
                     YGLRVTINTDNRLMSDTTVTKELLLCHQHYGWSLHTIKEIIIAGFKSAFMPYREKADL
                     LAEISRELAGFEDPRGEVTGRAGLVESDPAVTPPATPPAPAKPKLVSQP"
     gene            3452..4246
                     /locus_tag="VF516_20040"
     CDS             3452..4246
                     /locus_tag="VF516_20040"
                     /inference="COORDINATES: protein motif:HMM:NF019886.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8110036.1"
                     /translation="MATKVDRERLRSLVEPHWRRLYNFVFRLTLDRERAERYVFDIFE
                     HAAEQADQAPAAEPDRELWLVGIANRLLEERLPRQPEVDFDMLDETLRGEATRTDVVR
                     SLSDPQRDFLLWELKQGCMTAVVNCLPPGERAAFVVCHILKLPDDAAAKSLGITESAY
                     KVRLSRARTKVGDYLAPRCEHVNPMNPCRCPARVGTALHKGFIGKVSGEVSLRKQAEF
                     PYGRYGTGVGNEDVPMRDITAIYGNLPEPDPPPDLGDRLVSQLAPP"
BASE COUNT          638 a         1534 c         1486 g          642 t
ORIGIN      
        1 gaacaccggg atccgcggcc tcgacgtcgc ctcgatccag cacgcgatgc tcgacggcgt
       61 cgaccggacc cgcgcccggc tcggcgccgc cggccagctc gatcgcctcg acctcgcggt
      121 cgagctcgtc ggcgccccgc ccgcgctgcc gcgcaagacc ctgctgatcg gccgctggcg
      181 ccccgatcgc ttcgacgcct gggccggcga gatcgcccgc cgcggcgtga cccggacccg
      241 ctccatcgtc ctgccggcgg ccctgcgcgg caccgacctc gacatctacg tctatcacct
      301 gggcggcccc gccggcggcg tcgcccgccg gctcggcacc gcgcgcgacc ccaccctcgg
      361 gttcctgccg tggtcgcgcg gcggctactg ggccctggcg tgcaatcccg acgagtgctt
      421 cgtgatcgcc gcgacccgcg tgctgtgatc ggcgcggccg tcggcgggga cgccgtgcta
      481 agactcggct ctggcatgga tcccgtggtg tcgatcatcg cgcccgcgta caacgaggcc
      541 aagaacctcg cggcgttcct cgcggcgatc acgccggtgc tcgacgggct cggcgagccg
      601 tgggagatcg tgttcgtcga cgacggcagc cgcgacgaca cgctcggcat gctgctcgcg
      661 gcgcgcgcgc aggatagccg gatcaaggtg gtcgggctgg cgcgcaactt cggcaaggac
      721 gtcgcgctgt cggccgggct cgcccacgcg cgcggccgcg ccgtgatccc gatcgactgc
      781 gacctgcagc acccgccggc gttgatcccc gagctggtcg cgaagtggcg cgccggtgcg
      841 gacatggtga tcgcggtgcg cacgcggcgc agcgaggagg gcgtggttcg ccgcacggcc
      901 gccggggcgt actacgcggt gatgcggcgg atgacgcagg tcgagatccc gcgcaacgcg
      961 ggcgacttcc ggctgctcga tcgcaagatc gtcgacatca tcaaccagat gccggagcgc
     1021 tgccggttca tgaagggcat cttcgcctgg cccgggttca agaccgagag cgtggggttc
     1081 caggcggcgg ttcgcgccgg cggccgcagc agctggtcgc tgtggaagct gtggcggttc
     1141 gcgctcgacg ggctgttctc gttctcgacc gtgccgctca agctgtggac gtacctcggc
     1201 gcgctgttcg cgctcggcgc gttcatctac ctggcgatca cgctggtcca gaagctggtg
     1261 ttcgggatcg acgcgccggg ctatgcgtca ctgctgatcg tgatgctcgg gttcaacggc
     1321 ctgctgctgc tgtccaacgg gatccagggc gagtacatcg cgcggatctt cgaggaggtg
     1381 aagggcaggc cgctgttcgt cgtcgccggc acgtggggat tcgacgagcc ggcggtgcgc
     1441 gtcgcgccgg cggcgaccgc cgagccggcg gcgagccgcg acgcggtgtc gtagcgctgg
     1501 caaacactgg cggcagcggc agagctgttg cagcccggcg gcgccggacg gatagtcggt
     1561 cgatgaccac cggcgagatc acctacgacc tgctccagcg actgcccaag accgatctgc
     1621 actgccacct cgatggctcg ctgcggctgg acacggtgat cgacctggcg cgccaccagg
     1681 gggtcaagct gccgacgttc gaccgcggcg agctgcaccg gatgctggcg gccggcgagc
     1741 aggtggcgtc gctcgacgac tacctgcgcg cgttcgacat cacgctcagc gtgatgcagg
     1801 tcgaggacgc gctggagcgc gcggcgtacg agctcgccga ggacgcctgg cgcgagaacg
     1861 tgcgctacct cgaggtgcga tattcgccgc tgctgcacac ccgccagggg ctgcggccgg
     1921 cccaggtcgt cgaggcggtg ctgcgcgggc tgcggatggc caagcgcgag ctcgggatcc
     1981 gctacggcgt gatcctgtgc gcgatccgct cgctcgggcc ggagtccagc ctgcggatcg
     2041 ccgagctgtg catcgcgttc aagaaccgcg gcgtggtcgg gttcgatctc gcgggcagcg
     2101 agagcggcaa ccccgcgaag gtccacaagc acgcgttcca gctcgtggtc gacaacaaca
     2161 tcaactgcac ggcccacgcc ggcgagtcgt tcgggcccga ctcggtgcac caggcgatcc
     2221 acaagtgcgg cgcgcaccgg atcggccacg gcacccggct ggtcgagagc ggcgacctgc
     2281 tcaactacgt caacgaccac cggatcccgc tcgaggtctg cccgtcgtcg aacgtgcaga
     2341 cccacgcggc gaccggctgg gacacccacc cggtcgactt cttcgtcgac tacgggctgc
     2401 gggtgacgat caacaccgac aaccggctga tgtccgacac cacggtgacc aaggagctgc
     2461 tgctgtgcca ccagcactac ggctggtcgc tgcacacgat caaggagatc atcatcgccg
     2521 ggttcaagag cgcgttcatg ccgtaccgcg agaaggccga tctcctggcc gagatcagcc
     2581 gcgagctggc ggggttcgag gatccgcgcg gcgaggtcac cggccgggcc gggctggtcg
     2641 agagcgatcc ggcggtgacc ccgccggcga ccccgcccgc gcccgccaag cccaagctgg
     2701 tctcgcagcc ctagggccaa tgccggtcac ccagggcctc ttctcgacgt tcgccctaga
     2761 cgtggacctt gaccttgacc ccaccctgga cattgacggc aacgtagagg tctgacccca
     2821 ccctagacct tgccccttga ccaccgtcgg ccgtcctcga cgtcctgaag gtccaaggcg
     2881 acgaagaaga tctgaggtcg acgtcgaggc cgacggtggg gtcgacgtcg tacatcgccc
     2941 tcgacctcaa cgttcgcggg aacgtcaacg tcaaatcgtc aacgtcaacc acccttgcgt
     3001 cccgaatcgc ctctggcccc gtcttatctg accggcattg gccctagggc ggggcgtcga
     3061 tcgcggcatc cggcgcagca tccggggcgg catcctcgcc cgcgtcgatc gcggcgtccg
     3121 ccggggcatc ggtgctggtg gcatctactg gcagagctgc gtcggggcgt ccgcacacca
     3181 gatcgggcgg tgtgtcatct cggtggcagt acatgtccgc cgcgcagctg tatccgtctg
     3241 gacatgcgcc gcgcggtcca cacaggaacc cgcacgatgg cctcgggatc tcgtagcacg
     3301 cagcgatgat tgcgccgccg agcgcgagac cgacgcccag ggaccggagc cgtcccgaca
     3361 ccgtgcgacg atatcatgaa cactttttgc gtacccccgc ataccctggc acacgacgtc
     3421 aaacgcccat cgtggtgtac catctggata gatggctacg aaggtcgacc gcgagcggct
     3481 gcgttccctg gtcgagccgc actggcgccg cctgtacaac ttcgtgttcc ggctgacgct
     3541 ggatcgcgag cgcgccgagc gctacgtgtt cgacatcttc gagcacgccg ccgagcaggc
     3601 cgatcaggcc ccggccgcgg agcccgaccg cgagctctgg ctggtcggga tcgcgaaccg
     3661 gctgctcgag gagcggctgc cgcgccagcc cgaggtcgac ttcgacatgc tcgacgagac
     3721 gctgcgcggc gaggcgacgc gcaccgacgt cgtgcgctcg ctgagcgacc cgcagcgtga
     3781 cttcctgctg tgggagctca agcagggctg catgacggcg gtggtcaact gcctgccgcc
     3841 cggcgagcgc gccgcgttcg tggtctgcca catcctcaag ctgcccgacg acgccgccgc
     3901 gaagagcctc gggatcaccg agagcgccta caaggtgcgg ctgtcgcggg cgcgcacgaa
     3961 ggtcggcgac tacctcgcgc cgcgctgcga gcacgtcaac ccgatgaacc cgtgccgctg
     4021 cccggcgcgg gtcggcacgg cgctgcacaa gggcttcatc ggcaaggtct cgggcgaggt
     4081 ctcgctgcgc aagcaggccg agtttccgta cggccgctac ggcaccgggg tcggcaacga
     4141 ggacgtgccg atgcgcgaca tcaccgcgat ctacggcaac ctgcccgagc ccgatccgcc
     4201 gccggacctc ggcgaccggc tggtctcgca gctcgcgccg ccatgatccg cccgctcgcg
     4261 cctcgctcgc gccccgcggt ggcatgcacc ctggcgctcg
//
LOCUS       DASVOR010001040         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_361256,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001040 DASVOR010000000
VERSION     DASVOR010001040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361256"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..497)
                     /locus_tag="VF516_20045"
     CDS             complement(63..497)
                     /locus_tag="VF516_20045"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8110037.1"
                     /translation="MTRTCAACGAANRIPIAHLADTGRCGRCKAPLAASREPIEVGDA
                     TTFEQIRREAKVPVLVDFWATWCGPCRAVAPEVARAAADLAGRALVLKVDTERLPEIA
                     ERYRVQGIPNFVVLARGSVVDQHAGAMRHTDLVRLVDGALAA"
     gene            complement(572..1717)
                     /locus_tag="VF516_20050"
     CDS             complement(572..1717)
                     /locus_tag="VF516_20050"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8110038.1"
                     /translation="MKVGRLAIATIALLALGLPAAGCALSEAPAPAGTWSAASGTTTV
                     RVHYDTGFGNRIAIRGSAAPLSWSAGKDATWTSGNVWVASWSSSAGDLDLKPLINDAA
                     WSIGANYHVRAGATVDLYPFFHASAGSLMPVPSFSSPQLGNSRTLMIYLPPSYGENPL
                     KRYPVLYMHDGQNLFDAATSAFGTEWQVDENVDALVAGGRMDEVIVVGVYNTANRIWE
                     YTPCCDPTYGGGGADTYERFLIDTVKPFIDRSFRTLPGKDTTAIMGSSLGGLVSFYIA
                     RRNPSVFGKAGCMSSSFWWNNQALTVAVEQSTEHVPVAIYLDAGTDSDGLPETTRMDH
                     ALAADGYVQGRDLDFVTAQGGSHNEASWAARVATPLSWLFPWQSTAN"
     gene            1881..>2124
                     /locus_tag="VF516_20055"
     CDS             1881..>2124
                     /locus_tag="VF516_20055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110039.1"
                     /translation="MPRGSKAKYSSKQKRQAHHIEEGAKRRGYSPQRAAQIGYATVNK
                     QDGGGKKGGSGRGKQSTKSSSRAGGRASGRSRRGGSR"
BASE COUNT          324 a          752 c          771 g          277 t
ORIGIN      
        1 cggcgcgcgc atcaccgatc tagaaccggc cgacccggtc gcccccgccg gagcgcggtg
       61 gatcacgcgg cgagcgcgcc gtcgaccagc cgcacgaggt cggtgtgacg catcgcaccg
      121 gcgtgctggt cgaccaccga gccgcgggcg agcaccacga agttcgggat gccctgcacg
      181 cggtagcgct cggcgatctc gggcagccgc tcggtgtcga ccttgaggac cagcgcgcgc
      241 ccggcgaggt cggcggccgc gcgggcgacc tcgggggcca ccgcgcggca cgggccacac
      301 cacgtcgccc agaagtcgac cagcaccggc accttcgcct cgcggcggat ctgttcgaac
      361 gtcgtggcat cgccgacctc gatcggctcc cggctcgccg caagcggcgc cttgcagcgg
      421 ccgcaccggc cggtgtcggc gagatgcgcg atcgggatcc ggttcgcggc gccgcaggcg
      481 gcacaggttc gggtcatggc tccagcatgc gcacgccggg ccgcgggcgg accctcgccg
      541 atcgggcggt ctgtgcgcgc cggggccccg gtcagttcgc cgtgctctgc cagggaaaca
      601 gccagctcag cggcgtggcg acccgcgcgg cccacgacgc ctcgttgtgc gagccgccct
      661 gcgcggtgac gaagtcgaga tcgcggccct gcacgtagcc gtcggcggcc agcgcgtggt
      721 ccatccgcgt ggtctcgggc aggccgtcgc tgtcggtgcc ggcgtcgagg tagatcgcga
      781 ccggcacgtg ctcggtcgac tgctcgacgg ccacggtgag cgcctggttg ttccaccaga
      841 acgagctcga catgcagccg gccttgccga acacgctcgg gttgcgccgc gcgatgtaga
      901 acgacaccag gccgccgagc gacgagccca tgatcgcggt ggtgtccttg ccgggcaggg
      961 tgcggaagct ccgatcgatg aacggcttca cggtgtcgat caggaaccgc tcgtaggtgt
     1021 cggcgccgcc gccgccgtag gtcggatcgc agcacggcgt gtactcccag atccggttcg
     1081 cggtgttgta gacgccgacg acgatcacct cgtccatccg gccgccggcg accagcgcgt
     1141 cgacgttctc gtcgacctgc cactcggtgc cgaacgccga ggtcgccgcg tcgaacaggt
     1201 tctgaccgtc gtgcatgtag agcaccgggt agcgcttgag cgggttctcg ccgtagctcg
     1261 gcggcaggta gatcatcagc gtgcggctgt tgccgagctg gggcgacgag aagctcggga
     1321 ccggcatcag gctgccggcc gaggcgtgga agaacggata gaggtcgacc gtggcgccgg
     1381 cgcgcacgtg gtagttcgcc ccgatcgacc acgccgcgtc gttgatcagc ggcttgaggt
     1441 cgaggtcgcc ggccgagctg ctccacgagg cgacccagac attgcccgag gtccaggtcg
     1501 cgtccttgcc cgcgctccac gacagcggcg ccgccgagcc ccggatcgcg atccggttgc
     1561 cgaacccggt gtcgtagtgg acccggaccg tcgtcgtgcc ggacgccgcg ctccaggtgc
     1621 ccgccggcgc cggcgcctcc gagagcgcgc acccggcggc cggaaggccc agcgcgagca
     1681 acgcgatggt tgcgattgcg agtcgcccga ccttcatcga cgcagtcatc gcgcgcacgg
     1741 ataccaggat cgccgggcgc agggaatcgc cggcgcaccc gatcaccggc ggagatccgg
     1801 cccgcgcgtt agccgtgtgc ggcggacaac cgatctaaca cgtcacccga cacgggcacg
     1861 tgtcatgcac tctgcaaggc atgcccagag gaagcaaagc caagtactcg agcaagcaga
     1921 agcgtcaggc gcaccacatc gaggagggcg cgaagcggcg cggttactct ccgcagcgcg
     1981 cggcgcagat cggctacgcc accgtcaaca agcaggacgg cggcggcaag aaaggaggca
     2041 gcggccgcgg caagcagtcg accaagtcgt cgagccgcgc cggcggtcgg gccagcggtc
     2101 gcagccgccg cggcggctcg cgcg
//
LOCUS       DASVOR010001041         1509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156650,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001041 DASVOR010000000
VERSION     DASVOR010001041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156650"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1509
                     /locus_tag="VF516_20060"
     CDS             <1..>1509
                     /locus_tag="VF516_20060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEX8110040.1"
                     /translation="DSDEPADDDPPDTVVHVRDSGRQAKASTDDEVRAVRGSTRLEAK
                     RQRRREGRDTGRRRAPILTEAEFLARREAVDRVMVVRQRGDRTQIAVLEDDILVEHYV
                     TKASSTSYAGNVYLGRVQNVLPSMEAAFVDIGKGRNAVLYAGEVNWDVAGLEGRARSI
                     EHALKSGDNILVQVTKDPIGHKGARLTSQVSLPGRYLVYVPNGSMTGISRKLPDTERA
                     RLKDILRRIVPDDAGVIIRTAAEGASEEELTRDVERLKAQWETIQRKAAQTKSAPTLL
                     YAEPDLELRVVRDVFNEDFTKLIVSGDGAWEMISQYVDHVAPDLLPRLQRWTGEGDAF
                     HDLRIEEQLLKGLDRKVWLPSGGSLVIDRTEAMTVIDVNTGKFVGQGGNLEQTVTRNN
                     LEAAEEIVRQLRLRDIGGIIVIDFIDMVLESNRDLVLRRLTECLGRDRTKHQVAEVTS
                     LGLVQMTRKRVGQGLLEAFSEPCPTCGGRGVLVHTEPVDDSRKKAAEPAPRAG"
BASE COUNT          255 a          526 c          539 g          189 t
ORIGIN      
        1 gactccgacg agccggccga cgacgacccg ccggacaccg tggtgcacgt ccgcgacagc
       61 ggccgccagg ccaaggccag caccgacgac gaggtcaggg ccgtgcgcgg ctccacccgg
      121 ctggaggcca agcggcagcg tcgccgggag ggccgggaca ccggccggcg ccgcgcgccc
      181 atcctgaccg aggccgagtt cctcgcccgc cgcgaggccg tcgaccgcgt gatggtcgtc
      241 cgccagcgcg gtgaccgcac ccagatcgcg gtgctcgagg acgacatcct cgtcgagcac
      301 tacgtgacga aggcgagctc cacgtcgtac gcgggcaacg tctacctcgg tcgcgtgcag
      361 aacgtgctgc ccagcatgga ggcggcgttc gtcgatatcg gcaagggccg caacgcagtc
      421 ctgtacgcgg gcgaggtcaa ctgggacgtc gccggcctcg agggccgcgc tcgcagcatc
      481 gagcacgcgc tgaagtcagg cgacaacatc ctcgtgcagg tcaccaagga cccgatcggg
      541 cacaagggcg cgcgcctgac cagccaggtc agcctgcccg gccgctacct cgtgtacgtc
      601 ccgaacggct cgatgaccgg catcagccgc aagctgccgg acaccgagcg ggcgcggctg
      661 aaggacatcc tgcgccggat cgtgccggac gacgccggcg tgatcatccg taccgccgcc
      721 gagggcgcga gcgaggagga gctcactcgc gacgtcgagc ggctcaaggc gcagtgggag
      781 acgatccagc gcaaggcggc gcagaccaag tccgcgccca ccctgctgta tgccgagccg
      841 gaccttgagc tgcgggtcgt cagggacgtc ttcaacgagg acttcaccaa gctgatcgtg
      901 tccggtgacg gcgcctggga gatgatcagc cagtacgtcg accacgtggc gcccgatctg
      961 ctgcccaggc tgcagcgctg gaccggcgag ggcgacgcct tccacgacct gcgcatcgag
     1021 gagcagctgc tcaagggcct cgaccggaag gtctggctgc cctcgggcgg ttcgctggtc
     1081 atcgaccgca ccgaggcgat gacggtcatc gacgtgaaca ccggcaagtt cgtcggccag
     1141 ggcggcaacc tcgagcagac cgtgacccgc aacaacctgg aggcggccga ggagatcgtc
     1201 cgccagctcc ggctgcggga catcggcggc atcatcgtca tcgacttcat cgacatggtg
     1261 ctggagtcca accgcgacct ggtgctgcgt cggctgaccg agtgcctcgg ccgggaccgg
     1321 accaagcacc aggtcgccga ggtcacctcg ctggggttgg tgcagatgac ccgcaagcgg
     1381 gtcgggcagg ggctgctgga ggcgttctcc gagccgtgcc cgacctgcgg cggccggggt
     1441 gtgctcgtgc acaccgagcc ggtcgacgac tcgcgcaaga aggcggccga gccggcgccg
     1501 cgggccggc
//
LOCUS       DASVOR010001042         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_11118,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001042 DASVOR010000000
VERSION     DASVOR010001042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11118"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VF516_20065"
     CDS             <1..262
                     /locus_tag="VF516_20065"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8110041.1"
                     /translation="AEGPRAKLYNLRVEAEHRGRGGGEELVRAAAVAGERIGKRTLAL
                     EADDDGSGKLVRWYQSQGFRPAGETRDGKPAFEAPVRALKKP"
     gene            933..>2252
                     /locus_tag="VF516_20070"
     CDS             933..>2252
                     /locus_tag="VF516_20070"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8110042.1"
                     /translation="MRRKLWFGVVVGAIAACSSAAGPPPPPSGVVYTFPADGQLDVPL
                     GTHIVVTFSDPVIASAIAACSGTAAQPVGALCLVGPDGPVAASAMVIGGGRIVQLTAA
                     LAEGTTYAVYASRALAPAAGNLPASGPLVRFTTRSTRPSTAATTLVAVNGGSPDQPEA
                     FRPMFESSTIRLVFSQPLDPRSVALAPGRIELVDGSGAAVPARLLSDGIHVSIDPIDD
                     LVAKQSYQVKIGSDLTDLAGRPVTPVSVTLTPSRSFPGHPIVQMLHTRAAGDHGSAAP
                     HVAETRNAIAIDSPLIGSSAIAIQPDALAIELGDPTLDGPLAFTIRRGQRLRAGGLDV
                     QLGGVLPAGLHTGDILIELLSDGGGRIYRNPHQPGNQRPDNDRAPLLADLSLDLAIYA
                     VDAEGNAVLTQTVLGVQGSGVVIADDGVLDIEAAIAIDLDLLGLATTA"
BASE COUNT          316 a          830 c          793 g          313 t
ORIGIN      
        1 ggccgagggt ccgcgcgcca agctctacaa cctgcgcgtc gaggccgagc accgcggtcg
       61 cggcggcggc gaggagctgg tgcgcgcggc cgcggtcgcc ggcgagcgca tcggcaagcg
      121 cacgctcgcg ctcgaggccg acgacgacgg cagcggcaag ctcgtccgct ggtaccagag
      181 ccagggcttt cgcccggcgg gcgagacccg ggacggcaag ccggcgttcg aggcgcccgt
      241 acgcgcactg aagaagccct gagggagcga tcgctcggcg ccgccgtcgc ccgcgccgcc
      301 ggccgccagc ctcgcgcagc aacggccggg cattctcggg atcgatctgc tcggcgttgt
      361 gcagcgccgg cttgcgcgaa tcaccttcgg cacatcgatc ctcgtgaatg gctgtgtgtc
      421 aaagctcacg aactgggcaa cacactcgcg atctcgtgag ccgctgctac ggcgagcggc
      481 ctctccggga tcctgcacgc accggcagcc gtgtgcgcgt cccgggcggc ggagcggatc
      541 cgcgcgaaaa cccgagcgtc gatggcaagt tacaagaaat ctcgggatcc atgtcgatcc
      601 gcggcggggc cgtgcgtcgt ctcgcgagtt caagcgcgct gccgagctca ccggcaacgc
      661 tcgccagcgc gcccgcctgc tcgcccgcgc ggcggcgtgt gagaccaccg acgactccac
      721 gtgacgcgcg cccacgctcg ccgcgctcgc cggggccgct cgccccgaac atgaacggga
      781 gacagcggtc gaatgctgtt ccggtgcaat gatcgtgcgc cggagctcac ggtttatgtt
      841 ggcagcgtaa tctcggtgat agcgcgcatc cgcgagagcg aattgccaat ctcgagcagc
      901 gtagcaatcg caactggtga tagggtcgcg ccatgaggcg caagctctgg ttcggtgtgg
      961 tcgtgggggc gatcgcggcg tgctccagcg cggccggtcc gccgccgccg ccgtcgggtg
     1021 tggtctacac gtttcccgcc gatggtcagc tcgacgtgcc gctcggcacg cacatcgtcg
     1081 tgacgttctc ggacccggtg atcgcgagcg cgatcgccgc gtgctcgggg accgcggcgc
     1141 agccggtcgg cgcgctgtgc ctggtcggcc ccgacggccc ggtcgcggcg agcgccatgg
     1201 tgatcggcgg cggccggatc gtgcagctca ccgccgcgct cgccgagggc acgacgtacg
     1261 cggtgtacgc gagccgcgcg ctggcgccgg cggccggcaa cctgccggcg agcgggccgc
     1321 tggttcgctt caccacgcgc agcacccggc cgagcaccgc ggcgaccacc ctggtcgcgg
     1381 tcaacggcgg ctcaccggac cagcccgagg cgttccggcc gatgttcgag agctcgacga
     1441 tccggctggt gttctcgcag ccgctcgatc cgcgcagcgt cgccctcgcg cccggccgga
     1501 tcgagctcgt cgacgggtcc ggcgccgcgg tccccgcgcg cctgctgtcc gacggcattc
     1561 acgtgtcgat cgatccgatc gacgatctcg tggcgaagca gtcgtaccag gtgaagatcg
     1621 gcagtgatct gaccgacctc gccggccgcc cggtgacccc ggtgtcggtc acgctgacgc
     1681 cgagccgcag cttccccgga cacccgatcg tgcagatgct gcacacgcgc gccgccggcg
     1741 accacggctc cgccgcgccg cacgtcgccg agacgcgcaa cgcgatcgcg atcgacagtc
     1801 cgctgatcgg cagcagcgcg atcgcgatcc agcccgacgc gctcgcgatc gagctcggcg
     1861 atccgacgct cgacggcccg ctcgcgttca cgatccggcg cggccagcgc ctgcgcgccg
     1921 gcgggctcga cgtgcagctc ggcggcgtgc tgccggccgg gctccacacc ggcgacatcc
     1981 tgatcgagct gctcagcgat ggcggcggcc ggatctaccg caatccgcac cagccgggca
     2041 accagcgccc cgacaacgat cgcgctccgc tgctcgccga cctcagcctg gatctcgcga
     2101 tctacgcggt cgatgccgag ggcaacgccg tgctgaccca gaccgtgctc ggtgtgcagg
     2161 gctcgggcgt cgtgatcgcc gacgacggcg tgctcgacat cgaggccgcg atcgcgatcg
     2221 atctcgacct gctcgggctc gcgaccacgg ct
//
LOCUS       DASVOR010001043         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448656,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001043 DASVOR010000000
VERSION     DASVOR010001043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448656"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..646
                     /locus_tag="VF516_20075"
     CDS             <1..646
                     /locus_tag="VF516_20075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8110043.1"
                     /translation="AATASSPPTRPALSRVPTPPDSELRGFGEQRTPSGFVEQRTPGV
                     GEQTTPGQRTPTDPARPRTPTGSPLPSRVSTDNAAAAEEAFRRGELAMKRDQPGEAVL
                     EFKTACDLNPNEVDYAAMLAWAKFCAAGDKAAIGGDTRKALERAIYKSNKPERARFYL
                     GRVERMLGRDKEALRQFHEVLDLKPNHADAASEVRAIESRLQSAGKSGLFGRKR"
     gene            866..>2649
                     /locus_tag="VF516_20080"
     CDS             866..>2649
                     /locus_tag="VF516_20080"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX8110044.1"
                     /translation="MSTLSRLLLGAALASASCATGAGPTALTTHDAPIAPSSTDQMFA
                     SLRPTALSPSDATRTLDAAIASHFQRHATRRGYLMTDKPLYQPGETIWFRVDLRATGT
                     LIGAPPTGLTLQLVSPRGAVALQKRIQAVAGVAQNDFALDPGLDGGEYTLRLAADDGT
                     ADEKRVIVNTYEAPRLQKTLEFVRKAYGAGDAVAAAIEVKRATGEPFARRPLTGVVTV
                     DDAEIARLAITTDGDGKAIARFALPPAIARGDGLLTILADDGGVTESIQKRIPILVSS
                     LQLQMFPEGGDLVDGLPGRVYFLAKNPLGKPADVEGRVVDDRGQVVGELRSIHDGLGR
                     FDLQPATDRSYHLEITRPAGIAQKFDVPAAKPGGCVLRSVPARSADAIRVAATCTTSR
                     SLIVEAVLRETRLAAGAADVTAGAPALFELPVDPTAQGAVRVTLFSSKQEPLAERLVY
                     HGRGQDLTITLAADRKTYSPRDPVKLRLHATDPTGKPVKASLGVAVVDDTVLSFADDK
                     SAHILAHLYLEPELGATAADPIEEPNFYFSDKPEAPAAMDALLATRGYRRFEWQPVLS
                     PPPPPPPATVSAVAENEAPVEQLAAAGPP"
BASE COUNT          376 a         1074 c          893 g          306 t
ORIGIN      
        1 cgccgcgacc gccagctcgc cgccgacccg acccgcgctg tcgcgggtcc cgacgccccc
       61 cgactccgag ctgcgcgggt tcggcgagca gcgcacgcca tccggcttcg tcgagcagcg
      121 cacgccgggg gtcggcgagc agaccacccc ggggcagcgc acgccgaccg atccggcgcg
      181 gccccgcacc ccgaccggca gcccgctgcc atcgcgggtc tccaccgaca acgcggccgc
      241 cgccgaggag gcgttcaggc gcggcgagct cgcgatgaag cgcgaccagc ccggcgaggc
      301 ggtcctcgag ttcaagaccg cctgcgatct caaccccaac gaggtcgact acgccgccat
      361 gctcgcgtgg gccaagttct gcgccgccgg cgacaaggcc gcgatcggcg gcgacacccg
      421 caaggcgctg gagcgcgcga tctacaagtc gaacaagccc gagcgcgcgc ggttctacct
      481 cggccgcgtc gagcgcatgc tcggccgcga caaggaggcg ctccggcagt tccacgaggt
      541 gctcgatctc aagccgaacc acgccgacgc ggcgagcgag gtccgcgcga tcgagtcgcg
      601 cctgcagtcc gccggcaaga gcggcctgtt cggccgcaag cgctgagcgt aaccacccgc
      661 gcgacgcgac gcgcggaggg gtgccggagg cgtgaacgcc gcggctgggc cgcgaaacag
      721 gggacgggaa cgtcccctgt ggatcaaccg gccgcaagcg ctagcgatcc ggacggtttc
      781 gcggtgcggc cccatgcacc gcagcgcggc aggcgcgggc cgccagatcg tctgcccgcg
      841 cagcgcgttt gcaaccggca tccccatgtc caccctctcc cgcctcctgc tcggtgccgc
      901 cctggcatcg gcgagctgtg cgaccggcgc cggcccgacc gcgctgacca cgcacgacgc
      961 cccgatcgcc ccgagctcca ccgatcagat gttcgcctcg ctccggccga ccgcgctgtc
     1021 gccgagcgac gccacccgca cgctcgacgc cgcgatcgcg tcgcacttcc agcgccacgc
     1081 cacccgccgc ggctacctca tgaccgacaa gccgctgtac cagcccggcg agacgatctg
     1141 gttccgcgtc gacctgcgcg ccaccggcac gctgatcggc gcgccgccga ccgggctgac
     1201 cctgcagctg gtgtcgccgc gcggcgcggt cgcgctccag aagcggatcc aggccgtcgc
     1261 cggcgtcgcg cagaacgact tcgcgctcga tcccggcctc gacggcggcg agtacacgct
     1321 ccggctcgcc gccgacgacg gcaccgccga cgagaagcgg gtcatcgtca acacctacga
     1381 ggcgccgcgg ctgcagaaga cgctcgagtt cgtgcgcaag gcctacggcg cgggggatgc
     1441 ggtcgccgcg gcgatcgagg tcaagcgcgc caccggcgag ccgttcgccc ggcggccgct
     1501 gaccggcgtc gtcacggtcg acgacgccga gatcgcccgg ctcgcgatca cgaccgacgg
     1561 cgacggcaag gcgatcgccc ggttcgcgct gccgccggcg atcgcgcgcg gcgacggcct
     1621 gctcacgatc ctcgccgacg acggcggcgt gaccgagtcg atccagaagc ggatcccgat
     1681 cctcgtctcg tcgctgcagc tccagatgtt ccccgagggc ggcgatctgg tcgacggcct
     1741 gcccggccgg gtctacttcc tcgcgaagaa cccgctcggc aagcccgccg acgtcgaggg
     1801 ccgggtcgtc gacgaccgcg gccaggtcgt cggcgagctg cgctcgatcc acgacggcct
     1861 gggccggttc gatctccagc ccgccaccga ccgcagctac cacctcgaga tcacccggcc
     1921 cgccgggatc gcccagaagt tcgacgtgcc ggcggcgaag ccgggtggct gcgtgctgcg
     1981 cagcgtcccc gcgcgctcgg ccgacgcgat ccgcgtcgcc gcgacctgca ccacctcgcg
     2041 ctcgctgatc gtcgaggccg tgctgcgcga gacccggctc gccgccggcg ccgccgacgt
     2101 caccgccgga gcgcccgcgc tgttcgagct gccggtcgat ccgacggcgc agggtgccgt
     2161 gcgggtcacg ctgttctcgt cgaagcagga gccgctcgcc gagcgcctcg tctaccacgg
     2221 ccgcggccag gacctgacca tcacgctggc cgccgaccgc aagacctact cgccgcgcga
     2281 cccggtgaag ctgcggctgc acgccaccga ccccaccggc aagccggtca aggccagcct
     2341 gggcgtcgcc gtggtcgacg acaccgtgct gagcttcgcc gacgacaagt cggcccacat
     2401 cctcgcccac ctctacctcg agcccgagct cggcgcgacc gccgccgatc cgatcgagga
     2461 gcccaacttc tacttctccg acaagcccga ggcgccggcc gcgatggacg ccctgctcgc
     2521 cacccgcggc taccgccggt tcgagtggca gcccgtgctg tcgccgcccc cgcccccacc
     2581 gccggcgacc gtgtccgccg tggccgagaa cgaggcgccc gtggagcagc tcgccgccgc
     2641 tggcccccc
//
LOCUS       DASVOR010001044         5545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_536525,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001044 DASVOR010000000
VERSION     DASVOR010001044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5545
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536525"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VF516_20085"
     CDS             complement(<1..571)
                     /locus_tag="VF516_20085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110045.1"
                     /translation="MIELARLTFLRRSLHDPRAVAELDLQIVDLGETIEARLALADPP
                     LPLDRLRTAFQLEATEQRCLWLAFAHAVSSEVRALSGFDDGLSLEVLDRLVYASPRLR
                     DRFARELGPSGRLLRYGLLELGDGGGSRFSRTVRVADPVIELAHGVDELAGDIAGFAS
                     LCEPPRAELLVAPELAPAVRSALQHHIDHG"
     gene            804..2360
                     /locus_tag="VF516_20090"
     CDS             804..2360
                     /locus_tag="VF516_20090"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8110046.1"
                     /translation="MRDVRGRGDGLGIGRAGLAGGTGSPGPGKRTLTEALPVQARRAI
                     DAPASEAAGPGLDGEDDDAGSTSHRSRRFDGDPDLAPVAAGTRQLARGDRGPAVRKIQ
                     LALRDMGYSTRVDGVFDGDTEDQVKALQRSGGAFDGGKVNQATMVIFERRFTMRTPYV
                     EAARDEAPNLHLDQASGWGDKGWDAAHPPSALTRHTHTLSDPEKADALATLSPAARTM
                     PAYGGSGYKAKLDPMLRDIVRKKAKPAHDKAKDHKDPRKLFSLDLAAQIGKHSKQAVD
                     RVYGHWAAHKEFEPRTTLQDKFDAESKKIDGMTPDERFQLARDRLDYLWRTSDQVAAL
                     NATWAVDRSRAAEQADIDSVLDAVTRDLLEDLLAIQRAWPGSTSKGTVFVQLFKGKNA
                     AGNRSRRWHIFATLVHEYLHNLANPAWLTFRRHVRDQDKTKGHTLAEGVTEFLTRNVL
                     SAVDPAEPALRKAVEGDDYDADADPADDIDRGGGYQAEADRAEKLVSVVGVHNLYAAY
                     FVGQTQLVGG"
     gene            2362..2952
                     /locus_tag="VF516_20095"
     CDS             2362..2952
                     /locus_tag="VF516_20095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110047.1"
                     /translation="MRVTLALILAACAPGAHTAPHDDPRTRMTRSNPCADTLAALVAG
                     DLARVHGLPATCTLADLAAALSPLDGEGVSELGEPPARRRIRYVRAPGLAEPVQAWLD
                     GDRVVLVDVERPPTPHGWQAYVAPLGPPEARLDYAWSVLVLPGAEWVWPARGVVVAVK
                     DSTGAVLRTGVFVPGTLAEYRRVARYLSDERDEPGG"
     gene            complement(2978..4960)
                     /locus_tag="VF516_20100"
     CDS             complement(2978..4960)
                     /locus_tag="VF516_20100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110048.1"
                     /translation="MVWTSLVNTTVSGDVITKTGGQPWAEDAGAVSQQSIASGDAALQ
                     FTIDETSRFRFIGFAHTSDWHAANMDYSFRMQAGHADVYEHNTWQANILIAVGDVMKI
                     DVVGGVAHYYKNGVLQYTSAQAPVYPLGITTSMIDGSSTIASARISASGNAVTWVGQV
                     NTTVTGTVITKTGGQPWAEDAGAVSQQSIASGDGWFQFTIDETSRFRFVGFAHTTDWH
                     AANMDYSFRMQAGHADVYEHNTWQANILIAVGDVMKVDITGGVARYYKNGALQFTSAQ
                     APAYPLWVTTSMIDGSSTIASAQMAGAGTPPGNCGSGPFGGGAWPPGCWRPYATTSPF
                     NTPLPASPRLRSDSAGIVNRMLHFTADTSAAPNLAGGTQAANMIAPSDHMGGWPTYYG
                     RASDPVYTVTCSLGANCSLQANHIQFHAPAGAERQGGAAATSGQDRHMTIVDQTTGNE
                     IDLWQVDVAQLPAGGGTISTSWSGYTRIDGDGRAIGPGEGTAARIGNIAGRIRYEELA
                     AGVIDHAISIVVNCDNGTAVYPADTPNGQHCTDPSNAPPLGARFRLNMTWDQINALPV
                     PEWKKVVLRAMSKYGAFMNDTGSSFYFDWQTESGNQYTSQGAADPWLSFGAAHWGNNG
                     AGYTGTWHDNDDGINWRTQVWANLQVVDACVSNGGC"
BASE COUNT          803 a         2015 c         1953 g          774 t
ORIGIN      
        1 ggccgtgatc gatgtggtgc tgcagcgcgg agcgcaccgc cggggcgagc tccggggcga
       61 ccagcagctc cgcccgcggt ggctcgcaca ggctcgcgaa cccggcgatg tcgccggcga
      121 gctcgtcgac cccgtgggcg agctcgatca ccggatcggc cacccgcacc gtgcggctga
      181 accgcgatcc gccgccgtcg ccgagctcga gcaggccgta gcgcaagagc cggccgctcg
      241 gcccgagctc gcgcgcgaag cggtcgcgca gccgcggcga cgcgtagacc aggcgatcga
      301 ggacctcgag cgacagcccg tcgtcgaacc cgctcagcgc gcggacctcg gacgacaccg
      361 cgtgcgcgaa cgccagccac aggcagcgct gctcggtcgc ctcgagctgg aacgcggtgc
      421 gcagccggtc cagcggcagc ggcgggtcgg ccagcgccag ccgcgcctcg atggtctcgc
      481 cgagatcgac gatctgcagg tcgagctcgg cgaccgcgcg cgggtcgtgc agcgagcggc
      541 gcaagaacgt cagccgcgcg agctcgatca cgtcgtcgac atgatcgcgt atcgcccgat
      601 aagccatcac catccgcgcc cagcgtacgc gagcgcgggc cggcggcgcc atgtctcatc
      661 gccggtgccg cgggcggatc acgcggagac atgtctcatc gtccggtgac acgccgtgac
      721 gcatgacatg caatgatccg acaggcgatt cgatcgcact catgcgttgc aatcccacgc
      781 cgactcgcgc gagcatggcc gggatgcgcg acgttcgagg gcgtggcgat ggcctgggca
      841 tcggcagggc agggctcgca ggcggaaccg gctcgccggg gccgggcaag cgcaccctca
      901 ccgaggcgct gccggtgcag gccaggcgcg cgatcgatgc accggcgagc gaggcggccg
      961 gaccgggcct cgacggcgag gacgacgacg ccgggagcac cagccaccgc agccggcggt
     1021 tcgacggcga cccggatctc gccccggtgg ccgcgggcac ccggcagctg gccaggggcg
     1081 atcgcggccc cgccgtgcgc aagatccagc tcgcgctgcg cgacatggga tactcgaccc
     1141 gcgtggacgg cgtgttcgac ggcgacaccg aggaccaggt caaggcgctg cagcgcagcg
     1201 gcggtgcgtt cgacggcggc aaggtcaacc aggcgacgat ggtcatcttc gagcggcggt
     1261 tcacgatgcg cacgccgtac gtcgaggccg cccgggacga ggcgcccaac ctccacctcg
     1321 accaggcgag cgggtggggc gacaagggct gggacgcggc gcacccgccg agcgcgctga
     1381 cccgccacac ccacacgctg tcggaccccg agaaggccga cgcgctcgcg accctgtcac
     1441 cggcggcgcg gaccatgccg gcctacggcg gcagcggcta caaggccaag ctcgatccga
     1501 tgctgcgcga catcgtccgc aagaaggcca agcccgccca cgacaaggcc aaggatcaca
     1561 aggatcccag gaagctgttc tcgctcgacc tcgccgcgca gatcggcaag cactccaagc
     1621 aggccgtcga tcgggtctat ggccactggg ccgcgcacaa ggagttcgaa ccccgcacga
     1681 ccctgcagga caagttcgac gccgagtcga agaagatcga cggcatgacc cccgacgagc
     1741 gcttccagct cgccagggac cgcctcgatt acctgtggcg gacctccgac caggtcgcgg
     1801 cgctcaacgc gacgtgggcg gtcgatcgct cgcgcgccgc cgagcaggcc gacatcgaca
     1861 gcgtgctcga cgccgtcacc cgcgacctcc tcgaggacct gctcgcgatc cagcgggcct
     1921 ggccgggctc gaccagcaag ggcacggtgt tcgtgcagct gttcaagggc aagaacgcgg
     1981 ccggcaaccg cagccggcgc tggcacatct tcgcgaccct ggtccacgag tacctccaca
     2041 acctggccaa ccccgcgtgg ctcaccttcc ggcgccacgt gcgagaccag gacaagacca
     2101 agggccacac cctggccgag ggcgtcaccg agttcctgac ccgcaacgtg ctcagcgcgg
     2161 tcgacccggc ggagcccgcg ctgcgcaagg cggtcgaggg cgacgactac gacgccgacg
     2221 ccgacccggc cgacgacatc gaccgcggcg ggggatacca ggccgaggcc gatcgcgccg
     2281 agaagctggt gagcgtggtc ggcgtccaca acctttacgc cgcgtacttc gtcggccaga
     2341 cccagctcgt cggtggctga cgtgcgcgtc accctggccc tgatcctcgc ggcgtgcgcc
     2401 cccggcgcgc acaccgcgcc gcacgacgac ccgaggactc gcatgacccg atcgaatccc
     2461 tgtgccgaca ccctcgccgc cctcgtggcg ggcgatctgg cccgcgtgca cggcctgccc
     2521 gcgacctgca cgctcgccga tctcgccgcg gcgctgtcac cgctcgacgg cgagggcgtc
     2581 tcggagctgg gcgagccgcc cgcccgccgc cggatccgct acgtccgcgc acccggcctc
     2641 gccgagccgg tgcaggcctg gctcgacggc gatcgcgtcg tgctggtcga cgtcgagcgg
     2701 ccgccgacgc cgcacggctg gcaggcctac gtcgcgccgc tcggtccgcc cgaggcccgg
     2761 ctcgactacg cctggagcgt gctggtgctg cccggcgccg agtgggtgtg gccggcgcgc
     2821 ggcgtggtcg tcgcggtcaa ggacagcacc ggcgccgtgc tgcggaccgg cgtgttcgtc
     2881 cccggcacgc tcgccgagta ccgccgcgtc gcgcgctacc tctccgacga gcgcgacgag
     2941 cccggcggct gacggcccgg gctcgcgtcc gccgcggtca gcagccgccg ttcgagacgc
     3001 acgcgtcgac cacctggagg ttcgcccaga cctgggtgcg ccagttgatg ccgtcgtcgt
     3061 tgtcgtgcca ggtcccggtg taccccgcgc cgttgttgcc ccagtgggcg gcgccgaacg
     3121 acagccacgg atccgcggcg ccctggctgg tgtactggtt tcccgactcc gtctgccagt
     3181 cgaagtagaa actcgatccg gtgtcgttca tgaacgcgcc gtacttcgac atcgcgcgca
     3241 gcacgacctt cttccactcc gggaccggca gcgcgttgat ctgatcccag gtcatgttga
     3301 gccggaaccg cgcgccgagc ggcggtgcgt tgctggggtc ggtgcaatgc tggccgttcg
     3361 gcgtgtccgc cggatagacc gcggtgccgt tgtcgcagtt gacgacgatg ctgatcgcgt
     3421 ggtcgatcac gcccgctgcg agctcctcgt agcggatccg gccggcgatg ttgccgatcc
     3481 gcgcggcggt gccttccccg gggccgatcg cccggccatc gccgtcgatc cgggtgtacc
     3541 cggaccagct ggtcgagatc gtgccgccgc cggcgggcag ctgcgcgaca tcgacctgcc
     3601 acagatcgat ctcgtttccc gtggtctggt cgacgatcgt catgtggcgg tcctggccgg
     3661 aggtcgcggc ggcgccgccc tggcgctcgg cgcccgccgg tgcgtggaac tggatgtggt
     3721 tggcctggag cgagcaattg gcgccgagcg agcacgtcac cgtgtagacc ggatccgacg
     3781 ccctgccgta gtaggtcggc cagccgccca tgtggtccga cggcgcgatc atgttcgcgg
     3841 cctgcgtgcc cccggccagg ttcggcgccg ccgaggtgtc ggcggtgaag tgcagcatgc
     3901 gattgacgat gcccgcggag tcggaccgca gccgcgggct ggccggaagc ggcgtgttga
     3961 acgggctggt ggtcgcgtac ggccgccagc aacccggcgg ccaggccccg ccgccgaacg
     4021 ggccgctgcc gcagttgccc gggggcgtgc cggcgccggc catctgcgcg ctcgcgatcg
     4081 tcgagctgcc gtcgatcatc gaggtcgtga cccacagcgg atacgccggc gcctgtgcgc
     4141 tggtgaactg cagcgcgccg ttcttgtagt agcgcgcgac gccgccggtg atgtcgacct
     4201 tcatcacgtc gccgaccgcg atcaggatgt tggcctgcca cgtgttgtgc tcgtagacgt
     4261 cggcgtggcc ggcctgcatc cggaacgagt agtccatgtt cgccgcgtgc cagtcggtgg
     4321 tgtgggcgaa gccgacgaac cggaaccggc tggtctcgtc gatcgtgaac tggaaccagc
     4381 cgtcgcccga ggcgatcgac tgctgcgaca ccgcgccggc atcctcggcc cacggctgcc
     4441 cgccggtctt ggtgatcacc gtgccggtga ccgtggtgtt gacctggccg acccaggtca
     4501 ccgcgttgcc cgaggcgctg atccgcgccg acgcgatcgt cgagctgccg tcgatcatcg
     4561 acgtcgtgat ccccagcgga tacaccggcg cctgtgcgct ggtgtactgc aacacaccgt
     4621 tcttgtagta gtgcgcgacg ccaccgacca cgtcgatctt catcacgtcg ccgaccgcga
     4681 tcaggatgtt cgcctgccac gtgttgtgct cgtagacgtc ggcgtggccg gcctgcatcc
     4741 ggaacgagta gtccatgttc gccgcatgcc agtcgctggt atgggcgaag ccgatgaacc
     4801 ggaaccggct ggtctcgtcg atcgtgaact gcagcgccgc atcgccggac gcgatcgact
     4861 gctgcgacac cgcgccggcg tcctcggccc acggctgccc gccggtcttg gtgatcacgt
     4921 cgccgctgac cgtcgtgttg accagactgg tccacaccac gggcgcggtc gcctcgatcg
     4981 ccccggcggc ctgcccaacc ttcgcgccat cggcggggac acacgcagtc cacggcaggg
     5041 caacgacgat ccaccacgca catgttcgta tgctcatctc gcgactccct tggttggtta
     5101 ccggcgatcc gagcaacgtg cgtgccgcgg tgtgtgcgat cccgatcgcg cgaagccacg
     5161 caagcgcgca gcgcgcgcga tgccgcgagt cgctgccggc gtcgcatgtg gcgcaccgtc
     5221 acgctgctgc agaatctcgc gtggcgcccg tggcgcgctg gcggtgatcg cggtccgcgg
     5281 cgcgatcgtg gcgagcgcgg cgggtcgtcg tgcctgcgca atgttcgtac gcggacgatc
     5341 gaccagcgag cgatctcgcc ccggcggtgc cgctcgatct gctcgccggc caccagccgg
     5401 accaggaccg tggagcaggc cgaggcgcgg gccatggcgg ccatggatgg gtgccgcgcg
     5461 ccgtggtggg tcgccggcgg ctgggccctc gagctgcacg ccgcggcgca cggccaccgg
     5521 aaccggccac atgccgatct cgaga
//
LOCUS       DASVOR010001045         3294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_361285,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001045 DASVOR010000000
VERSION     DASVOR010001045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361285"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2122)
                     /locus_tag="VF516_20105"
     CDS             complement(<1..2122)
                     /locus_tag="VF516_20105"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8110049.1"
                     /translation="MTFTAIAGRERQHAIVVMLGSAGADGGQVPVEPALWQTIGLHGG
                     RLEQLADGSTIVVFEADRHAATDHVARAARCALALRGVANGRPMAIAMGRVESIRELS
                     GGDVLDRARRLLSHAASAPGAPAWIALDEVSAGLLDARFEVTERDVGLMLCGERALIQ
                     GARTLLGRPTPCVGRDWELEALTGILDECIDESKARAVMLLGPAGIGKSRVGAEFMSR
                     AQERHGELVIWRGRGDSLRAGSTLDLLAQALRGTIGLRGSEPLAERRDKIRPRVAEHV
                     PAIEQERVTQFLGELVGAPFPGDGEGGAALRAARQDAQLMGEQMYKAWLDFLQAETAV
                     HPVLLLLEDLHWSDFGTMQFIGTALRDLRDSPWMVLALARPELYEVFPQLWAERQVQE
                     IRIKGLGRRASERLVRQVLGTGAGPNTIERLVQQADGNALYLEELIRAVAEGKDSDAP
                     LPETVLAMVETRLARLPRDARRVLRVASVFGEVCWERGVAALLEGAMAGKVGELLATL
                     VEQEVLVARRDSRFPGECELAFRHALLREGAYATLTADDQGLAHRRAGEWLVQRGEGD
                     PMALAGHFERGGQGARAAEQAFFVLDVDATMARAGLGLVCAPPTELRMALLGIRCELS
                     AYRWQLISDTMPEAEELIRSASRGSVLWAQGMTTYICGTLLAGRMDELLASIALLREV
                     DLAPDAVGKMGLVLVISICVLDNLG"
     gene            2549..>3294
                     /locus_tag="VF516_20110"
     CDS             2549..>3294
                     /locus_tag="VF516_20110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110050.1"
                     /translation="MARARGCRQEEGGELKTDLVVTVEPRESSSLMALADLLCDRFEI
                     EQQIQGGGMGEVFRARDRVSGQTVAIKVIFDARDRRAARFVREIQLLSELSHPGIVRY
                     ICHGATPSGALFLAMEWLDGEALRCRLEREPLTVSESVALATRVAEALGAAHARGIVH
                     RDLKPSNLFLIEGRIERVKLIDFGIARKGGLPPLTQTGMPIGTPGYMAPEQACSEGEL
                     DARADVFALGCVLFQCLTGTLPFKGDSAIA"
BASE COUNT          499 a         1197 c         1097 g          501 t
ORIGIN      
        1 gtccgaggtt gtcgaggacg cagatcgaga tcaccagaac gagccccatc ttgccgaccg
       61 catcgggggc gaggtcgacc tcgcggagca gcgcgatcga cgccagcagc tcgtccatcc
      121 tgccggcgag cagcgtgccg caaatgtacg tcgtcatgcc ctgtgcccag agcacggagc
      181 cacgcgacgc ggagcgaatc agctcctcgg cctccggcat cgtgtcggag atgagctgcc
      241 agcggtaggc cgatagctcg cagcggatgc cgagcagcgc catccggagc tcggtgggag
      301 gggcgcagac caggccgagt ccggcgcggg ccatggtggc gtcgacgtcg aggacgaaga
      361 acgcctgctc ggcggcgcga gcgccctgtc ctccgcgctc gaagtgcccg gccagggcca
      421 tgggatcgcc ttctccgcgc tgcacgagcc actcgccggc gcggcggtgc gcgagcccct
      481 gatcgtcggc ggtcagggtg gcgtaggccc cctcgcggag cagcgcgtgg cggaacgcga
      541 gctcgcactc gccggggaac cggctgtctc gccgcgcaac cagcacctcc tgctcgacga
      601 gcgtcgcgag caattcgccc accttccctg ccatggcccc ttcgagcaac gcggccacac
      661 cgcgttccca gcacacctcg ccgaacacac tcgcgacccg aagcacccgc cgcgcatcgc
      721 gcgggagccg cgcgagccgg gtctcgacca tggccagcac ggtctcgggc aagggggcat
      781 cgctgtcctt gccctccgcc acggcgcgga tcagctcctc caggtagagc gcgttgccat
      841 cggcctgttg caccaggcgc tcgatcgtgt tcggccccgc gcccgtgccc agcacctgcc
      901 gcaccagtcg ctcgctcgct ctgcgcccga ggcccttgat ccggatctcc tgtacctggc
      961 gctccgccca cagctgcggg aagacctcgt acagctcggg ccgcgccagc gcgagcacca
     1021 tccacgggct gtcgcggagg tctcgcagcg ccgtgccgat gaactgcatg gtcccgaaat
     1081 cgctccagtg caggtcctcg agcaggagca ggaccggatg cacagcggtc tcggcctgca
     1141 ggaagtccag ccacgccttg tacatctgct cgcccatgag ctgggcgtcc tgccgcgctg
     1201 ctcggagggc agcgccgccc tcgccatcgc cggggaacgg ggcgccgacg agctccccca
     1261 ggaactgggt cacccgctcc tgctcgatcg cgggcacgtg ctcggcgacc cggggccgga
     1321 tcttgtcccg tcgctcggcg agcggctcgc ttccccggag cccgatggtc ccgcgtagcg
     1381 cctgggcgag cagatcgagc gtcgagcctg cgcgcagcga gtcgccgcgc ccacgccaga
     1441 tcacgagctc gccatggcgc tcctgggcgc ggctcatgaa ctcggcgccg acccgcgact
     1501 tgccgatccc cgccggtccg agcagcatca cggcacgtgc cttcgactcg tcgatacact
     1561 cgtcgaggat cccggtcagc gcctcgagct cccagtcgcg cccgacgcac ggcgtcggac
     1621 ggccgaggag cgttctcgct ccctggatca gcgcgcgctc accgcacagc atcagcccga
     1681 cgtcgcgctc ggtcacctcg aaccgcgcgt cgagcagacc ggcgctcacc tcgtcgagcg
     1741 cgatccacgc cggggcaccc ggtgcgctcg ccgcgtgcga cagcagccgc cgcgcgcggt
     1801 cgagcacatc gccgccggac agctcgcgga tcgactcgac ccggcccatc gcgatcgcca
     1861 tcgggcggcc gttggcgacc ccgcgcaacg cgagcgcgca gcgcgcagcc cgggcgacat
     1921 gatcggtcgc ggcgtgccgg tccgcctcga acaccacgat cgtcgagccg tccgcgagct
     1981 gctcgagacg gccgccatgc aggccgatcg tttgccacag cgccggctcg accggcacct
     2041 gaccgccgtc cgccccggcg ctccccagca tcaccacgat cgcgtgctgg cgttcgcggc
     2101 cggcgatggc cgtgaacgtc acgcgatgtg ccctcggcgc cgcggatcac cgcgtcctgg
     2161 tgcgctgccg cccggtcgcc ggggggccgt acgagggagc gtaagcatgt cgccgtcctg
     2221 ttccacggat gtacgcacac aatagtccaa cctcccgcct tgcgcgaggc acatccagcg
     2281 aagtgggatc cgcaagcgcc agggacgaac cagcggcatt tcgcctgtgg tggtggggaa
     2341 gcgtctgtga gagtagtcgg caccctccat gccaacgtga ctcagaaact cacgggtcga
     2401 aatcactccg tcgccgcggc cgaggagtaa gtcgttctcg tcagcgttct attggtgact
     2461 tgatgatatg tgtgccggtc gcgctcgctg ggtagtcttc gtaggtgcga cgatcaccgc
     2521 agcccggtcc gttcgatgag gtgcttccgt ggctcgagcg cgcggttgcc gccaagaaga
     2581 aggaggtgag ttgaagacgg accttgtcgt taccgtcgag ccgagggaga gttcgagcct
     2641 catggcgctt gcggatctgc tctgcgatcg gttcgagatc gagcaacaga tccagggcgg
     2701 cggcatggga gaggtattcc gtgctcgcga tcgagtatcg ggccagaccg tcgcaatcaa
     2761 ggtcatcttc gacgctcggg atcgtcgcgc tgcgcggttc gtgcgcgaga tccagttgct
     2821 ctccgagctg tcgcaccctg gcatcgtccg ctacatctgt cacggagcca ctccgtccgg
     2881 cgctctgttc ctggccatgg agtggctcga cggcgaggct ctccggtgcc gcctggagcg
     2941 cgagcccctg acggtgagcg agtccgttgc tctcgccacc cgggtcgccg aagcgcttgg
     3001 cgccgctcac gcccgtggca tcgttcatcg cgacctcaag ccctcgaacc tgttcctgat
     3061 cgagggccgc atcgaacggg tcaagctgat cgacttcggc atcgcccgca agggaggact
     3121 gccgccgttg acccagaccg ggatgccgat cgggacgcca ggctacatgg cgcccgagca
     3181 ggcatgcagc gagggagagc tcgacgcccg cgccgacgtg ttcgcgctgg gctgcgtgct
     3241 gttccagtgc ctgaccggaa ctctcccgtt caagggcgac agcgccatcg ccat
//
LOCUS       DASVOR010001046         3099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_11141,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001046 DASVOR010000000
VERSION     DASVOR010001046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11141"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1670
                     /locus_tag="VF516_20115"
     CDS             <1..1670
                     /locus_tag="VF516_20115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110051.1"
                     /translation="MAQGTTAASGQISQASSAAGAHISQAGTQVAAQLHQGAVIAGQQ
                     VTQAGTEATTQIDQQGADFQDKARQGVGDARTAMTTTVAQAGADQQRNVGTFESKKAS
                     ASSEIASKYAAEKANADAQAASEKQANAQDAGQAFLELILPASWTAAIKKAFASVFGN
                     WLGGFVYGLLSAGLAILICIGFCALFGPFAWIPALILLAGAVGLGIYSRFKTFSAYNG
                     RGPGFWEGVGLVCLGILDITGVPSIIEGLVGYKAFSNGHKMDDFEAGESVGSGLAALI
                     ALVWGGAKYFKSGKPTVDPKIDPKVDPKVDPKVDPKVDPKVDPKPDPKPEPKPEPDPA
                     EVAARQAKAKAIGDAAGDTAADTAAKDALAKVKLGKREAKNPAKIEAEVKRLVADQAK
                     EAGKKAADDALAKGASEADAKAAADAAAKAAADARAKRLMELSKDPAHANEVDVKSLR
                     EGEVGLEMEESGKLDGPLTRDPNPKGGEFIDKHGNVWDVKRPQSKFPKAGKTVVDGAM
                     EDIHESLAKTPPEKVVIDPTDLTPKDLADLKAEVTKQGLDNDVKYGK"
     gene            1667..2239
                     /locus_tag="VF516_20120"
     CDS             1667..2239
                     /locus_tag="VF516_20120"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8110052.1"
                     /translation="MTSELESMLARHEQGEDTLELKNADLRGMDLSGRDLTHIVLYES
                     QLDRVSLRGADLYGSNLSGSSLVEADLRGAQLSKSEFAGANLCGAHLEGAIANRIGLD
                     GADLRGADLTEADIGGAALVGADLRGAILRGTGLDRVLFDRTRVYAADLRGATGLDGA
                     TVVSIDIGETEPVRLDGDAARAWLVDRISS"
BASE COUNT          564 a         1056 c         1039 g          440 t
ORIGIN      
        1 cgatggcgca gggcaccacc gcggcgagcg gtcagatcag ccaggccagc agcgccgcgg
       61 gcgcgcacat cagtcaggcc ggcacccagg tcgccgcgca gctgcatcag ggcgccgtga
      121 tcgcgggaca gcaggtcacg caggccggca ccgaggcgac cacgcagatc gatcagcagg
      181 gcgccgactt ccaggacaag gcccgccaag gcgtcggcga cgcgcgcacc gcgatgacca
      241 ccaccgtcgc acaggccggc gccgaccagc agcgcaacgt cggcaccttc gagagcaaga
      301 aggcgagcgc gagctcggag atcgcgtcga agtacgcggc ggagaaggcc aacgccgacg
      361 cgcaggcggc gtcggagaag caggccaacg cgcaggacgc gggtcaggcg ttcctcgagc
      421 tgatcctgcc cgcgtcgtgg acggccgcga tcaagaaggc gttcgcctcg gtgttcggca
      481 actggctggg gggctttgtc tacggcctgc tcagcgccgg cctcgcgatc ctcatctgca
      541 tcgggttctg cgcgctgttc gggcccttcg cctggatccc cgcgctcatc ctgctggccg
      601 gcgcggtggg cctcgggatc tactcgcgct tcaagacatt ctcggcctac aacggacgcg
      661 gtcccgggtt ctgggaaggc gtcgggctgg tctgcctcgg catcctcgac atcacgggcg
      721 tccccagcat catcgagggc ctggttggat acaaggcgtt ctccaacggc cacaagatgg
      781 acgacttcga ggcgggtgag tcggtcggca gcggcctcgc cgcgctgatc gcgctcgtct
      841 ggggtggcgc gaagtacttc aagtccggca agcccaccgt cgatccgaag atcgatccga
      901 aggtggatcc caaggtcgat cccaaggtcg atcccaaggt cgatcccaag gtcgacccca
      961 agccggatcc caaacccgag cccaagcccg agccggatcc cgccgaggtc gcggcccgcc
     1021 aggccaaggc caaggcgatc ggcgacgccg ccggcgacac cgcggccgac accgccgcga
     1081 aggacgcctt ggcgaaggtc aagctcggca agcgcgaggc caagaatcca gccaagatcg
     1141 aggccgaggt caagcggctc gtcgccgatc aggccaagga ggccggaaag aaagcggccg
     1201 acgacgcgct cgcgaagggc gcgagcgaag ccgacgcgaa ggcggcggcc gatgccgcgg
     1261 cgaaggcggc cgcagacgcg cgcgcgaagc ggctcatgga actgtccaag gatccagcgc
     1321 acgcgaacga ggtcgacgtc aagagcttgc gcgagggcga ggtcggcctg gagatggagg
     1381 agagcgggaa gctcgacggt ccgttgacgc gcgacccgaa ccccaagggt ggcgagttca
     1441 tcgacaagca cggcaacgtc tgggacgtca agcggcccca atccaagttc ccgaaagcag
     1501 gcaagaccgt cgtcgacggc gccatggaag acatccacga gtccctcgcc aagacgcctc
     1561 ccgagaaggt ggtcatcgat ccgacggacc tgactccgaa ggatctggcg gatctgaagg
     1621 ccgaggtcac caagcaaggc ctcgacaacg acgtaaagta cgggaaatga cgagcgagct
     1681 cgaatcgatg ctggcgcggc acgagcaggg cgaagacacg ctcgagctca agaacgccga
     1741 cctgcgcgga atggacctgt ccggccgcga tctcacgcac atcgtgctct acgagtcgca
     1801 gctcgaccgc gtgtcgctcc gcggtgccga cctctacggc tccaacctct ccggcagctc
     1861 gctcgtcgag gccgacctcc gcggcgcgca gctcagcaag tcagagttcg ccggcgcgaa
     1921 cttgtgtggt gcacacctcg aaggtgcgat cgccaaccgt atcggtctcg acggcgccga
     1981 tctgcgcggt gccgatctga ccgaggccga catcggcggc gccgcgctgg tcggagccga
     2041 tctgcgcggc gccatcctgc gcggcaccgg gctcgatcgc gtgctgttcg accgcactcg
     2101 cgtctacgcc gcggacctcc gcggtgcgac cggcctcgat ggcgcgactg tcgtgtcgat
     2161 cgacatcggc gagaccgagc cggtccggct cgacggcgac gccgcacgtg cgtggctcgt
     2221 cgatcggatc tcgtcgtagg gcgcgccaat cgggcgcgcc aatcgggcgc gcccgtcagg
     2281 cgcggcggtt ggccgacgtc ggcagcgctt cccagcgcgc gtgaccgtcc ggccgactcg
     2341 cgccgatagc catgccgggg agccgcgacc tgcttgacgg cgcgcgacgc tcggggcatg
     2401 gccaagcgcc gcacttcatc atcccgacgc acccactgga ccgaatccga cgcacgacgc
     2461 gtcctcgcgg agtggtgcgg cgccactcac gagcatcccc gctcacgcct agcttgatcg
     2521 gcccgtcgga ccacacgcgc acgaagtcga cgatcgtgtc gcggatgtca gggggtaccc
     2581 agcgaccgct cagggctgtt ggagtaggaa tcgcgatcgc tcacgacccc ctcctcggat
     2641 ccggacgtgc ggagttgcac catcgtatgt tggcgccaac atacgatgcg ttatgtggcg
     2701 aagttgtagg cgaaggccct gtaattcttg ccacttgacc agctccgcca catgaccggg
     2761 ctcgccacat aacgcatccg gctcttgcct tgggtatgac gtcgaagcgc ttgctcggcc
     2821 aggcattggt agatccgggg aagtgggagc ccgctcgcaa ggcgattcat cctctcccat
     2881 gtcacgcgac ctcgttggct gcggcgcgac agcgaccgcc gccagagacc gatgacgtgg
     2941 tgtcgaaacg ccccgagcct atcgtagttt ccaggcactc cgtagtaacg atagtgcccg
     3001 tccagcacgc tacttaggta gctcctctgc tctgggattg agtcgtgcat gcgccaccac
     3061 agctcggtct ttaccgcacg tagctttgca tgccagcgt
//
LOCUS       DASVOR010001047         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448681,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001047 DASVOR010000000
VERSION     DASVOR010001047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448681"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VF516_20125"
     CDS             <1..303
                     /locus_tag="VF516_20125"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110053.1"
                     /translation="INFPDPWFKRRHHDRRMVDAALADGIAQVARPDADIFVQSDVWD
                     IALDAMATFERDERFVNQAGDWTFWRGGNPYGVRSWREQNAEDTGLVIWRILYRRR"
     gene            complement(686..1330)
                     /locus_tag="VF516_20130"
     CDS             complement(686..1330)
                     /locus_tag="VF516_20130"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEX8110054.1"
                     /translation="MRKKSRLFALAEALRARRTGVTAQELADRFGVTLRTIYRDLEAL
                     QDAGMPIKADRGRGGGYALDKTYQLPPVNFTAREAALLVALARLATEQRLIPFPAAIE
                     RAVDKVKSALSTSAQRELLRLVGELQLVGVPARPVPLTIQTAVETAWFESRALRIVYA
                     KANGPTSPRLVRIRNLVFDRSETLLNCIDLETGSDRQFRLDRIQHAQVMDSQPS"
     gene            1392..1778
                     /locus_tag="VF516_20135"
     CDS             1392..1778
                     /locus_tag="VF516_20135"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8110055.1"
                     /translation="MSKPKSKEDRISWFEIPVTDLEKSAALYGKALGIELKREVLFGV
                     PHAILMAEGEAVTGTLISDPKRAPRRGVGTVVYLYTAGGVAPALARAVQAGAKVVQPT
                     TDIAPFGEIALFEDFDGNVVGLHTRK"
BASE COUNT          309 a          650 c          664 g          281 t
ORIGIN      
        1 atcaacttcc ccgatccgtg gttcaagcgc cgccaccacg atcggcgcat ggtcgacgcc
       61 gcgctcgccg atggcatcgc gcaggtcgcg cggcccgacg cggatatctt cgtgcagagc
      121 gacgtctggg acatcgcgct cgatgcgatg gcgacgttcg agcgcgacga gcggttcgtc
      181 aaccaggccg gcgactggac gttctggcgc ggcggcaatc cgtacggcgt gcggtcgtgg
      241 cgcgagcaga acgccgagga caccggcctg gtgatctggc ggatcctcta ccgccggcgg
      301 tgaagaggta tcgatccaag aggagggagg ccgaccaaga ggacgcagcc gatcctggaa
      361 atcttcgacc ttgtcctcga cgatgacctt gacccgaacc ttgacgtcga cggcaacgat
      421 gacgtctgac cccacccttg accttgcacc tggaccgcgg tgtgaaacag ccgctcgctg
      481 gcggagccgc aacgtcccga tcgccgcgat gtggacggag atgagctgag gtcgacgccg
      541 aggtcgacgg tgggggcaac gtcgtacgtc gccgtcaacc tcgacgttcg gcgtcgacgt
      601 cgacgtcgag ccgtcaacgt cgaccaccgc ttgtctcccg aaatcgccct tccctcttcc
      661 ctcgacactt cctgatggag cctgcctagc tcggctgcga gtccatcacc tgggcgtgct
      721 ggatccggtc gagccggaac tggcggtcgc tgccggtctc gagatcgatg cagttgagca
      781 gggtctccga gcggtcgaac accagattgc ggatccggac caggcgcggg ctggtggggc
      841 cgttggcctt ggcgtagacg atgcgcagcg cgcgcgactc gaaccacgcg gtctcgaccg
      901 cggtctggat cgtcagcggc accggccgcg ccggcacgcc gaccagctgg agctcgccga
      961 ccagccggag cagctcgcgc tgggccgacg tcgacagggc cgacttgacc ttgtcgacgg
     1021 cgcgctcgat cgccgccggg aacgggatca gccgctgctc ggtggccagg cgcgccaggg
     1081 cgaccagcag cgcggcctcg cgcgcggtga agttgaccgg cggcagctgg taggtcttgt
     1141 cgagcgcgta gccgccgccg cggccgcgat cggccttgat cggcatgccg gcgtcctgca
     1201 gcgcctccag gtcgcgatag atcgtgcgca gcgtgacgcc gaaccgatcg gcgagctcct
     1261 gggcggtcac gccggtgcgc cgggcgcgca gcgcctcggc cagcgcgaac agtcgggact
     1321 tcttccgcat gaaagatgac aaaaggctgt cacaacccgg ggccaaggtc tagctcgaaa
     1381 gggacaaacc catgagcaag cccaagagca aggaagaccg catcagctgg ttcgagatcc
     1441 cggtcaccga cctggagaag tccgccgcac tgtacggcaa ggcactcggc atcgagctca
     1501 agcgcgaggt gctgttcggc gtgccgcacg cgatcctgat ggccgagggc gaggcggtca
     1561 ccggcaccct gatcagcgac cccaagcggg cgccgcggcg gggcgtcggc acggtggtgt
     1621 acctgtacac ggccggcggg gtggctccgg cgctggcccg cgcggtccag gcgggggcca
     1681 aggtcgtgca gccgaccacg gacatcgccc cgttcgggga gatcgcgctg ttcgaggact
     1741 tcgacggcaa cgtcgtcggc ctgcacacgc ggaagtgacg cgccgcctgc ccggctttcc
     1801 ccctgcgagg tcgccatgag ccgctccgag ttccccctga tcgcttcgac atcccgggcc
     1861 gccgagccgg ccgccgagct ggtcgccggg cctccgatcc gagc
//
LOCUS       DASVOR010001048         2170 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_68762,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001048 DASVOR010000000
VERSION     DASVOR010001048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68762"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VF516_20140"
     CDS             complement(<1..1344)
                     /locus_tag="VF516_20140"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEX8110056.1"
                     /translation="MSEASTLRAMRWIAFVVALVAAGTARADQPHAEFRLGDRDTPHV
                     GVPFHLDLLIEGFDETPQPDVPKLTLPGADVTFVAAQPNVSRGIQIINGRRSDFVKVT
                     WVLRWQIEPHKEGRLRIPALTVSQGDKRVVTEPAETDVDTIPVTDAMKLSLALPDRPI
                     FVGETVPVTLTWLFHNQPADDPRWSIPLMTMDAFTVSGPPAGTTRKPLVVPAGGKELQ
                     LPYTVDQVDQGGTAYNRLTTTFFLAPRKVGKLDVPASSVVVALAVGRADFFGNAPSRL
                     FRAADVARTLEVKPLPETDRPGNFAGAVGEQFAIDVSTSRSVVKLGEPVELAIRVKSN
                     QRLDTLALGRLDGEGGLPRDRFVVPADPPTGELSDDGKTKTFKVTVQVTGPATEVPAI
                     AFSYFDPPKATYQTIHSEPIALSVAGSAVVGAGDVVAARPTPPAGAPAARPAGVDA"
     gene            complement(1344..>2170)
                     /locus_tag="VF516_20145"
     CDS             complement(1344..>2170)
                     /locus_tag="VF516_20145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110057.1"
                     /translation="ADPKQAEAQQLEQARQQLEQARQQMARAEELRGQAQTALAALDK
                     ALAGTGDPMPPAKQADAELAELRKLFFSVIEHLQELIREQGDTRDQTSAANGEDDFTR
                     GPKLPGLITRQEGHAQMAKAITEALAQQADAAAKQPAQGQGQGPNAKSLSAAADEVRQ
                     SQGDMADARANLVKARDSKTATESLAPGVKSQGKALEHLENALRLLQPPPQKNNQNDK
                     DQQQQQQQQQQQQKQKQQDQQPQQGGAGQRARDEDARRQRERRDRDAQNDPVEKDW"
BASE COUNT          232 a          752 c          821 g          365 t
ORIGIN      
        1 cgcgtcgacg ccggcggggc gggcggccgg agcgccggcg ggcggggtcg ggcgggccgc
       61 gaccacgtca ccggcgccga ccacggccga tccggcgacc gacagcgcga tcggctcgga
      121 gtggatcgtc tggtaggtcg ccttgggcgg atcgaagtac gagaacgcga tcgccgggac
      181 ctcggtcgcc ggcccggtga cctgcacggt gaccttgaac gtcttggtct tgccgtcgtc
      241 ggacagctcg ccggtcggcg ggtcggcggg caccacgaac cggtccctgg gcaggccgcc
      301 ctcgccgtcg agccggccga gcgccagcgt gtcgagccgc tggttgctct tgacccggat
      361 cgcgagctcg accggctcgc cgagcttgac caccgagcgc gaggtcgaca cgtcgatcgc
      421 gaactgctcg ccgaccgcgc ccgcgaagtt gccgggccgg tcggtctcgg gcagcggctt
      481 gacctcgagc gtgcgtgcca cgtcggccgc gcggaacagc cggctcggcg cgttgccgaa
      541 gaagtcggcg cggccgaccg ccagcgccac gacgaccgac gacgccggca cgtcgagctt
      601 gccgaccttg cggggtgcga ggaagaacgt cgtggtcagc cggttgtacg cggtgccgcc
      661 ctggtcgacc tgatcgacgg tgtacggcag ctggagctcc ttgccaccgg ccgggaccac
      721 cagcggcttg cgcgtggtgc cggccggcgg cccgctgacg gtgaacgcgt ccatggtcat
      781 cagcgggatc gaccatcgcg gatcgtcggc gggctggttg tggaacagcc aggtcagcgt
      841 gaccggcacg gtctcgccga cgaagatcgg tcggtcgggc agggcgagcg acagcttcat
      901 cgcgtcggtg accgggatcg tgtcgacgtc ggtctcggcg ggctcggtga cgacccgctt
      961 gtcgccctgc gacacggtca gcgccgggat ccgcagccgg ccctccttgt ggggctcgat
     1021 ctgccagcgc agcacccagg tcaccttgac gaagtcgctg cgccggccgt tgatgatctg
     1081 gatcccgcgc gacacgttgg gctgcgcggc cacgaacgtc acgtcggcgc cgggcagggt
     1141 gagcttgggg acgtcgggct gcggggtctc gtcgaagccc tcgatcagga ggtcgaggtg
     1201 aaacggcacg ccgacgtgcg gcgtgtcgcg gtcgccgagc cggaactcgg cgtggggctg
     1261 gtcggcgcgc gcggtgcccg ccgccaccag cgcgaccacg aacgcgatcc accgcatcgc
     1321 gcggagggta ctcgcttcgc tcatcaccag tccttctcca ccggatcgtt ctgcgcgtcg
     1381 cggtcgcggc gctcgcgctg gcggcgggcg tcctcgtcgc gggcgcgctg gccggcgccg
     1441 ccctgctggg gctgctggtc ctgctgcttc tgcttctgtt gctgctgctg ttgttgttgc
     1501 tgctgctgct gatccttgtc gttctggttg ttcttctgcg gcggcggctg cagcagccgg
     1561 agcgcgttct ccaggtgctc cagcgccttg ccctggctct tgacgccggg ggcgaggctc
     1621 tcggtcgcgg tcttgctgtc gcgcgccttg accaggttgg cgcgcgcatc ggccatgtcg
     1681 ccctgcgact ggcggacctc gtcggccgcg gcggacagcg acttggcatt gggcccctga
     1741 ccctggccct gcgccggctg cttggcggcg gcgtcggctt gctgggccag cgcctcggtg
     1801 atcgccttgg ccatctgggc gtggccctcc tggcgggtga tcaggccggg cagcttgggg
     1861 ccgcgcgtga agtcgtcctc gccgttggcg gccgaggtct ggtcgcgggt gtcgccctgc
     1921 tcgcggatca gctcctggag gtgctcgatc accgagaaga acagcttgcg cagctcggcg
     1981 agctccgcgt cggcctgctt cgctggcggc atcggatcgc cggtgccggc cagcgccttg
     2041 tcgagcgccg cgagcgcggt ctgcgcctgg ccgcgcagct cctcggcgcg ggccatctgc
     2101 tgcctggcct gctcgagctg ctgcctggcc tgctcgagct gctgcgcctc ggcctgcttg
     2161 gggtccgcgg
//
LOCUS       DASVOR010001049        13632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_127270,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001049 DASVOR010000000
VERSION     DASVOR010001049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13632
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127270"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF516_20150"
     CDS             <1..502
                     /locus_tag="VF516_20150"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8110058.1"
                     /translation="AVADPSAPSAATMAPSAIEGAARLLTDLGLTAQRPFRVIDAEGV
                     PSDVADANPEILRLSGRALAVDGVREVIWRHGQELADLRRRSLLKRLLFLFASAPGKV
                     FSKEAIVQAVWNVDYHPLRHDAALFTNIMRIRRLLGEDGSEIIRVTEDGYRFVPPRDF
                     LFVIPR"
     gene            559..1152
                     /locus_tag="VF516_20155"
     CDS             559..1152
                     /locus_tag="VF516_20155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110059.1"
                     /translation="MKPAKSAGATARRAAGKPAAQWPAKGVTKRAAKQAVKRAAKKEV
                     KRAAKQATRRRGAWLAFVEQHGVVLASARGPVPSVAEAVAGQPIVGSWWAHPRGKAIF
                     AALSELDDSHDVRCFKLIDGKVTFVHRRVWPALACLAGYGVIAAERAAAIQQEHMPTG
                     EHRNLVTPYPDWVPDDVARAAAALTVEAARAQLGPWV"
     gene            complement(1270..1515)
                     /locus_tag="VF516_20160"
     CDS             complement(1270..1515)
                     /locus_tag="VF516_20160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110060.1"
                     /translation="MNTLDNASLDNVTGGAVVRTSSTSDAITQSLTSLQTTVSQLANN
                     NNNTSNNSNNLLLPMAMMIAMNRRQPTIVSGGATVVG"
     gene            1720..2859
                     /locus_tag="VF516_20165"
     CDS             1720..2859
                     /locus_tag="VF516_20165"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8110061.1"
                     /translation="MRVETKLVHAGEPRPRHQRAVAMPIYQSSTFEYAGQADYHDIQY
                     IRLNNTPNHAALHSKLAALEGTEAALVASSGMAAISSTLLSLLAAGDHVLAHDGLYGG
                     TYDLLTQDLPRLGITTTFIDAARPETWAAAATPRTRVFYVETLTNPLIQLCALDEVAR
                     FARERGMVSVIDNTLATPINFRPVEHGFDLIVHSATKYLNGHSDIVAGAVAGSAARVT
                     AVKHKLDHLGGALDPHACYLLHRGLKTLAVRVRYQCQSALEIASFLARHPRVVQVHYP
                     GLERHPDHARARQWLAGTGGMLSFELDGGAAAADQLFGRLTHPLSAPSLGGPETLITR
                     PATTSHAGLAPEVRRRQGITDGLIRLSVGLESTQDLIDDFAAALG"
     gene            3125..5854
                     /locus_tag="VF516_20170"
     CDS             3125..5854
                     /locus_tag="VF516_20170"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110062.1"
                     /translation="MACLDENVIAAYFSLKLSPRETESLFAHIDRCASCAQLFEAVGQ
                     GQSRDRESLSRAATWPGQPALDPAPATMLGRYRIERILGMGAMGVVYAGRDPRLDRSV
                     AIKLLRPHPSISGELQRARLIREAQAMAQLSHPNVIHVHEIGSFGEQLFIVMELIDGT
                     TLTDWLRAQPRTWREILQAFIAACAGLDAAHRAGIVHRDFKPDNVLVASDGRICVTDF
                     GLALWRPQLDDAVAASPSQGMTSITRPGAIVGTLAYMAPEQLRGELADTRSDVFSFCV
                     ALHEALLRTRPYRGRTVAELQAAIEHRRLAAGHRRGPGWLKRAVVRGLEPRPADRYAS
                     LGELLAALRADPAPRRRRAVLGLGIAAVVASSAYFGHDRALRGQVCVGAQDKLAGIWD
                     AHRKQALRDAFTRTRQPGANAVATATVDALDRYAQDWVTMHTGACEATRVRGEQSEEL
                     LDLRMQCLHVRAVELRALTDRFATADGDVIDHALDGVRALSALADCNDTEELRAGARR
                     PSDPRLRAEVEALEETVARARVQYYGSGLRQSLELATRVATQPAAAAYPPVRAQALAL
                     RGMSLTVLQQLDEAEPVLKEAAQLGLSCRADRVVVQSWLALATLDSWRHRLDPALEWS
                     RLAQSVIVRLGDDRLELKRVHSLVEIDRMNDRPREQLAAAQRGLALAERLQPPDEHEL
                     ALSLYDLAVAQHLVTGDLEVAAALERRALAIYERHPDENLRELDRTLTAIDLTLAALG
                     RYPEAEAMARRALALDERIFPADHPSLGYSLTNLADVLVKRERFADAARTLDRAEPIV
                     AGHRDALGDLVPQMVWLRGMVELGLGHLTRATELLEQALAQRELTGSDRAQVELALAQ
                     ARWQRGDRRRALEIAMQATAHVENAVPRDEITSRAIAQWIAERSRRPEARHSP"
     gene            5917..6798
                     /locus_tag="VF516_20175"
     CDS             5917..6798
                     /locus_tag="VF516_20175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8110063.1"
                     /translation="MVCWETLTAAFGPTSSDPALSGVLQGLYTAGRVRWAELPALPPE
                     DFVAHLALRVPPDVDPVAHLRGLVAEDLYLACACTCGIPDAVKAFDAQFLSRVPDYVS
                     HIGGSSDFGQEIQQQLRERLLVRRGDAPPRIADYSGRGSLASWLRVAAVRVALNHRVR
                     PNEARRVDDEAIVDELATEVSPQLDVLRARYAAVLGDALKRAVAGLRPDQRVLLRMYF
                     VSGHSTQQIATLLRVDRSTAARRLVAARQAVFDETHRWLRTELAIDSSEFASLARTLH
                     DQLNVSLGGLLADSPEG"
     gene            6952..7380
                     /locus_tag="VF516_20180"
     CDS             6952..7380
                     /locus_tag="VF516_20180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110064.1"
                     /translation="MIAAMTTAVAAAAWARSLPASTGQSLGPEQATCFHQYGNLGQVE
                     NTCSFTNRFCVPLVVDSTWHDVDVTVTAPDINHNVNCFAETFYPDSSFAMWSGFQAAS
                     RFGAGQTLVFTGLSVPGGGYMLSCCDVAPNAWVDSFNYSN"
     gene            7417..8031
                     /locus_tag="VF516_20185"
     CDS             7417..8031
                     /locus_tag="VF516_20185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110065.1"
                     /translation="MSVAAVGVGGYIVHNQALAELHREIAAARATAAVASRPPPVPVI
                     AAQTAPALAMPSHAPAQPSTEDQASPPRNPEGGSQSASRWKEEALAQRTAIEAGFAAE
                     VIDGAWAAAARRDLRDRISALLPSTSSLADVDCRASMCRVELVHREAAGSQRFMQAAF
                     FDPKDRVWSGPGFAIPAETGSDGTVTVVLYLARDGASFPMPDTR"
     gene            complement(8117..8347)
                     /locus_tag="VF516_20190"
     CDS             complement(8117..8347)
                     /locus_tag="VF516_20190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110066.1"
                     /translation="MAALCSCDSEREAVERVAEVEHAQWMAWSKEVAPEVSPARRARW
                     MKYWVPYKDLPEEVKELDRVWARKAIEAARRR"
     gene            8684..8818
                     /locus_tag="VF516_20195"
     CDS             8684..8818
                     /locus_tag="VF516_20195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110067.1"
                     /translation="MHYEADVFPGGLTVRSRDPDPTTTAYGMLTDLVGLLRSGRNSAT
                     "
     gene            8956..9723
                     /locus_tag="VF516_20200"
     CDS             8956..9723
                     /locus_tag="VF516_20200"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8110068.1"
                     /translation="MTAPIDHVAKNRAYWATLAAEYAATADRAWVAREPTWGIWEVPE
                     AELGVLPADLTGLDAIELGCGTGYVSAWLARRGARPVGIDNSPEQLATARRMQASHGL
                     TFPLHLGNAEATPFDDASFDLAISEYGASIWCDPYRWIPEASRLLRPGGQLIFLRNST
                     LLMLTVPEANGVAATDRLVRPLRGMHRLIWPEPSGVEFHISPGELIRLLRRTGFELED
                     MIEVYAPEGGTTRYPFVDLPWSRSWPCEEVWKARKWL"
     gene            9776..9940
                     /locus_tag="VF516_20205"
     CDS             9776..9940
                     /locus_tag="VF516_20205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEX8110069.1"
                     /translation="MKKYRCSICSHIYDPAEGDPDTGIPPGTPFEKLPDSWCCPDCGA
                     SKEDFEPMED"
     gene            complement(9979..11841)
                     /locus_tag="VF516_20210"
     CDS             complement(9979..11841)
                     /locus_tag="VF516_20210"
                     /inference="COORDINATES: protein motif:HMM:NF012855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004198 - calcium-dependent cysteine-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C2 family cysteine protease"
                     /protein_id="HEX8110070.1"
                     /translation="MKRFGANPLLKPSLLQSITQDAWAAYVTAVTPYFKIDDNDHERQ
                     FATFYTVYSRSKSLSSAYQGEFTEKQRTLAAAINGQLDREKIELYQSSTGHYEPWFAP
                     SLTMQSDPEILEKHRDTSRHELMEDPHHAPLFTALPDIDDVRQGYLGDCYLLAAVASI
                     VQIDPSHFVRNMVDNVDGTVTVKLYADVGQAFEIAIAKSIVIGEGSTRYARNSLWVQI
                     LEKAYASSGLQKENKDRKRKNNEPRNDSPPSYQDIAGGDPGDALLHLTGKPPSSFDLG
                     TSRARFGHEAPVRLQQAGEHIKKQIQQLEDEETLLKKQIEHKREQQVDVTTDESRLKR
                     IGEEIEGLVQQLVEMDEPILDLSNRLKLVFVSDTSLQALVRKHPNNEPLKQLVQQANS
                     MQLLPGEIGSGRYGTDESEAFKRIKSGIDRNRSMCASTRDTISDSENEEENKKGMCGE
                     SKVKGLAGKHAYSLLDYKPRPDPKPGEQILIKLRNPWGHYGRTYEKIDGKLKPVAFEG
                     NGVFWIDLADLTANFSSIEWTEQPPPPRPRREVETLKGSFYNDTRSDFRRAILEIAED
                     ADLGDWGDELCDAIERGLPFESRDAMLRVVLSLIPKRPNFAERLVDYLVQASVS"
     gene            complement(12093..12278)
                     /locus_tag="VF516_20215"
     CDS             complement(12093..12278)
                     /locus_tag="VF516_20215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110071.1"
                     /translation="MARAEETAPTWRASYLWRVPEDGKALGVAGSPVTPPSQPPPPCC
                     GQDAHVAIPAVQRGYIA"
     gene            12308..12988
                     /locus_tag="VF516_20220"
     CDS             12308..12988
                     /locus_tag="VF516_20220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110072.1"
                     /translation="MSAAQQILDQACSLARIAVLEADPAVYAERLAAELPAVATLIGL
                     AARDAALVAALGQIDAMVARAMRIRLDHALASDTSIGAPTRSVFASTITGYAGRLSLL
                     EQRARDIAVRGRAADPDLVAKSVVEAGCAVLALRDALRAGVLALIRDLAREAVTEADR
                     RARDRSLDESERRRWSAARRDLEAVAAEPERVLTAPMAARLGALPDQLDEPGMDREVA
                     FADLLELD"
     gene            13070..13399
                     /locus_tag="VF516_20225"
     CDS             13070..13399
                     /locus_tag="VF516_20225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110073.1"
                     /translation="MPILEVTMSYSPLAFLYAFSTPTITINETRDRRPWGTYRFELPP
                     GNYRVEMSYPWLISPECGKNSVDIVLREGDHKHVRYTARMLRYWPGRVSVDDVIPTAR
                     ALRDRST"
BASE COUNT         1941 a         4794 c         4721 g         2176 t
ORIGIN      
        1 ggcggtcgcc gatccgtcgg cgccgagcgc ggcgaccatg gcgccgagcg cgatcgaggg
       61 tgcggctcgc ctgctcaccg acctcggcct gaccgcgcag cgtccgttcc gggtgatcga
      121 cgccgagggc gtgccgagcg acgtcgccga cgccaacccc gagatcctgc ggctgtcggg
      181 ccgcgcgctc gcggtcgacg gcgtccgcga ggtgatctgg cgccacggcc aggagctcgc
      241 cgatctgcgc cgccgctcgc tgctcaagcg cctgctgttc ctgttcgcgt cggcgcccgg
      301 caaggtgttc tccaaggagg cgatcgtcca ggcggtgtgg aacgtcgact accaccccct
      361 gcgtcacgac gccgcgctgt tcaccaacat catgcgaatc cgccggttgc tgggcgagga
      421 cggatcggag atcatccggg tcaccgagga cggctaccgg ttcgtgccgc cccgggactt
      481 cctcttcgtc attccccggt gaaggcccca tcgaacaccg cagcgtcccg ctcggccagc
      541 cgtcgaggcg cccggcgcgt gaagcccgcg aagtcggccg gagcgaccgc acgacgggcc
      601 gcgggcaagc cggccgccca gtggccggcc aagggagtta ccaagcgggc agccaagcaa
      661 gcggtcaagc gggcggccaa gaaagaggtc aagcgggcag ccaagcaagc gaccaggcgg
      721 cgcggggcct ggctcgcgtt cgtcgagcag cacggtgtcg tcctcgcgtc ggcgcgcggg
      781 ccggtgccgt cggtcgccga ggcggtcgcc ggccagccga tcgtcggcag ctggtgggcg
      841 caccccaggg gcaaggcgat cttcgccgcg ctgtccgagc tcgacgactc ccacgacgtg
      901 cgctgcttca agctgatcga cggcaaggtc accttcgtcc atcgccgggt gtggccggcg
      961 ctcgcctgcc tcgccggcta cggggtgatc gccgccgagc gcgcggccgc gatccagcag
     1021 gagcacatgc cgaccggcga gcaccgcaac ctggtcacgc cgtaccccga ctgggtcccc
     1081 gacgacgtcg cgcgtgctgc ggcggcgctc accgtcgagg cggcgcgcgc ccagctcggc
     1141 ccgtgggtct aggccggggg ccggggccga aaaaattcag cgaggcgggt gtggcccggg
     1201 gcccaaccgg accacaccct cgaccgagtc accacagctc ggtcgttctc gccggagacg
     1261 ttgcgcggct cagccgacga ccgtcgcccc gccggacacg atggtcggct ggcggcggtt
     1321 catcgcgatc atcatcgcca tcggcagcag caggttgttg ctgttgttgc tggtgttgtt
     1381 gttgttgttc gccagctgcg agaccgtggt ctgcagcgag gtcagcgact gcgtgatcgc
     1441 gtcgctggtg ctgctggtgc ggaccacggc gccgccggtg acgttgtcga gagaagcgtt
     1501 gtcgagcgtg ttcatggcgg gactcctttg gttgatgcag agccatactg cagcgcccgt
     1561 gccggccgcg atgcgcgtcg aatcgcgggg ttccagcggt ccgggcgtaa actcgacgct
     1621 gcgaaccgag tcctggggcg gacgatcagt tgccgggcgc gcccggcgat cagcgggccg
     1681 tccggttgac tcgccgcggc aacggggcat cgtcggggaa tgcgagtcga aaccaagctg
     1741 gtccatgccg gcgagccacg gccccgtcac cagcgcgccg tcgccatgcc gatctaccaa
     1801 tcatcgacct tcgagtacgc cggacaggcc gattaccacg acatccagta catccggctc
     1861 aacaacacgc ccaaccacgc cgcgctgcac agcaagctcg cggcgctgga gggcaccgag
     1921 gcggcgctgg tcgccagcag cggcatggcg gcgatctcgt ccacgctgct gtcgctgctc
     1981 gccgcgggcg accacgtgct ggcgcacgac ggcctgtacg gcggcacgta cgacctgctg
     2041 acccaggacc tgccgcggct cggcatcacc acgacgttca tcgacgccgc ccggcccgag
     2101 acctgggcgg cggcggcgac cccgcgcacc cgggtgttct acgtcgagac cctgaccaac
     2161 ccgctgatcc agctgtgcgc gctcgacgag gtcgcgcggt tcgcgcgcga gcgcggcatg
     2221 gtctcggtga tcgacaacac gctcgcgacg ccgatcaact tccggccggt cgagcatggc
     2281 ttcgacctga tcgtccacag cgcgaccaag tacctcaacg gccactccga catcgtcgcc
     2341 ggcgcggtcg ccggcagcgc ggcgcgggtg accgcggtca agcacaagct cgaccacctg
     2401 ggcggcgcgc tcgacccgca cgcgtgctac ctgctgcacc gcgggctcaa gacgctcgcg
     2461 gtgcgcgtgc gctaccagtg ccagagcgcg ctcgagatcg cgagcttcct ggcccgccac
     2521 ccgcgggtcg tccaggtcca ctacccgggc ctggagcgcc atccggacca cgcgcgggcc
     2581 cggcagtggc tcgccgggac gggcggcatg ctgagcttcg agctcgacgg cggcgcggcc
     2641 gcggccgacc agctgttcgg ccggctcacc cacccgctgt ccgctccgag cctcggcggc
     2701 cccgagaccc tgatcacgcg gccggccacg acctcgcacg cggggcttgc gcccgaggtc
     2761 cggcgccgcc agggcatcac cgacgggctg atccggctgt cggtcggcct ggagtcgacc
     2821 caggacctga tcgacgactt cgccgccgcg ctcggctgac cggcgaccgc ggcgcgagcg
     2881 cgcacttccg cgtcatcgcg cgggccagcg ctgccgcgtc ggtccaaccg taggccccga
     2941 tcgtgcctcg gacgagggtg ctccgcacgc tgccgatcgc ccatcatgct gacggaacag
     3001 acatccgaag cagcgtttgc tgcacaaagc cgctcggatt cgcgaaatga ccaaattcct
     3061 tgcgactcca ctggcatgca acgttccatc cggggttggg cacggcgcga ggtagcatcg
     3121 ttccgtggcg tgtctggacg agaatgtcat tgctgcctac ttcagcctga agctgtcgcc
     3181 tcgcgagacc gagtcgctgt tcgcgcacat cgaccgctgc gcgtcgtgcg cgcagctgtt
     3241 cgaggcggtg ggccagggcc agagccgcga tcgcgaatca ctctcgcgag ccgcgacctg
     3301 gccgggccag cccgccctgg atcccgcacc cgcgacgatg ctcgggcgct atcgcatcga
     3361 gcgcatcctc ggcatgggcg cgatgggcgt ggtctacgcc gggcgcgacc cgcggctcga
     3421 tcgcagcgtc gcgatcaagc tcctgcgccc gcacccgagc atctccgggg agctgcagcg
     3481 cgcgcgcctg atccgcgagg cgcaggcgat ggcgcagctg tcgcatccca acgtgatcca
     3541 cgtccacgag atcggcagct tcggcgagca gctgttcatc gtgatggagc tgatcgacgg
     3601 tacgacgctg accgactggc tgcgtgcgca gccgcggacc tggcgcgaga tcctgcaggc
     3661 cttcatcgca gcgtgcgcgg gtctcgacgc cgcgcatcgc gccggaatcg tgcaccgcga
     3721 cttcaagccc gacaacgtgc tggtcgcgag cgacgggcgg atctgcgtga ccgacttcgg
     3781 tctcgcgctg tggcgcccgc agctcgacga tgcggtggca gcgtcaccgt cccaggggat
     3841 gacatcgatc acgcgcccgg gagcgatcgt cggcacgctg gcgtacatgg cgcccgagca
     3901 gctgcgcggt gagctcgccg acacgcgatc cgacgtgttc agcttctgcg tcgcgctcca
     3961 cgaggcgctg ctccgtacgc gtccctaccg cggcaggacc gtggccgagt tgcaggccgc
     4021 gatcgagcac cgccggctcg cagcgggtca caggcgagga ccgggctggc tcaagcgggc
     4081 ggtggtccgc gggctcgagc cccggccggc cgatcgctac gcatcgctcg gggagctgct
     4141 cgcggcgctg cgcgccgatc ccgcgccgcg ccggcggcgc gcggtcctgg gcctcggcat
     4201 cgccgcggtg gtggcgtcgt cggcgtactt cggtcatgac cgcgcacttc gcggccaggt
     4261 ctgcgtgggc gcgcaggaca agctggccgg gatctgggac gcgcaccgca agcaagcgct
     4321 gcgcgacgcc ttcaccagga cccggcagcc gggtgcgaac gcggtcgcga cggcgacggt
     4381 ggacgcgctc gaccgctacg cgcaggactg ggtcacgatg cacaccggtg cctgcgaggc
     4441 gacgcgtgtg cgcggcgagc aatccgagga gctcctcgac ctgcgcatgc agtgcctgca
     4501 cgttcgggcc gtcgagctgc gcgcgttgac cgaccggttc gccaccgcgg acggcgacgt
     4561 gatcgaccat gcgctcgacg gcgtgcgcgc gctctcggcg ctcgccgatt gcaacgatac
     4621 cgaggagctc cgggcgggcg cgcggcggcc gtccgaccct cgcctgcgcg ccgaggtcga
     4681 ggcgctcgag gagacggtcg cgcgcgcccg cgtgcagtat tacggctccg gcctgcgcca
     4741 gtcgctcgag ctggccacgc gcgtggcgac ccagccggca gccgcggcgt atccgccggt
     4801 gcgcgcccag gcgctcgcgc tgcggggcat gtcgctgacg gtgctgcagc agctcgacga
     4861 ggcggagccg gtgctcaagg aggcggccca gctcggcctc agctgccggg ccgatcgcgt
     4921 ggtcgtgcag agctggttgg cgctggcgac gctcgactcg tggcgccatc ggctcgatcc
     4981 tgcgctcgaa tggagccgcc tggcgcagtc ggtgatcgtg cggctcggcg atgaccggct
     5041 cgagctcaag cgcgtgcaca gcctggtcga gatcgaccgc atgaatgatc gcccgcgcga
     5101 gcagctggca gcggcgcagc gcgggctggc gctggccgag cgactccagc cgcccgacga
     5161 acacgagctc gcgctgagcc tctacgatct cgcggtcgcg cagcacctcg tcaccggcga
     5221 cctcgaggtg gcggccgcgc tcgagcgacg ggcgctggcg atctacgagc gccatcccga
     5281 cgagaacctg cgcgagctcg accgcacctt gacggcgatc gacctgacgc tggcggcgct
     5341 gggccgttat cccgaggccg aggcgatggc gcggcgggcg ctggccctcg atgagcggat
     5401 cttcccggcc gatcatccct cgctgggcta ctcgctgacc aacctggcgg atgtgctggt
     5461 caagcgcgag cggttcgccg acgcggcccg gacgctcgat cgggcggagc cgatcgtggc
     5521 gggccaccgc gacgcgctcg gcgatctcgt gccgcagatg gtgtggctcc gggggatggt
     5581 ggagctgggc ctggggcacc tcactcgcgc gaccgagctc ctggagcagg cgctcgccca
     5641 gcgcgagctg accgggagcg atcgagcgca ggtggagctg gccctcgcac aggcacgctg
     5701 gcagcgcggt gatcggcgcc gcgccctcga gatcgcgatg caggccacgg cacacgtgga
     5761 gaacgccgtg ccgcgggacg agatcacgtc gcgcgcgatc gcgcagtgga tcgccgagcg
     5821 atcgcggcgt cccgaggcca gacactcgcc ttgacgacgc ttggcgtacg ttgcgcgtcg
     5881 tgatagatgg atgcaatttt tcggacctgg aggagcatgg tgtgctggga gacgctgacc
     5941 gccgcgttcg gtccaacgag ctcggatcct gcgctgtccg gggtgctgca aggcctgtac
     6001 accgccggcc gtgtgcgctg ggcagagctg cctgcgctgc ctccagagga cttcgtcgcg
     6061 cacctcgcgc tgcgggtgcc gccggatgtg gatcccgtgg cgcacctgcg cggcctggtt
     6121 gccgaggatc tctacctggc ctgtgcgtgc acctgcggca tcccggacgc ggtgaaggcg
     6181 ttcgacgccc agttcctgtc gcgcgtgccc gactacgtga gccatatcgg cggatcttcg
     6241 gacttcggtc aggagatcca gcagcagctg cgcgagcgct tgctcgttcg ccgcggcgac
     6301 gcacccccgc ggatcgccga ctactccggg cgcgggtcgc tcgcgagctg gctccgcgtt
     6361 gcggcagtgc gggtcgcgct caaccaccgc gttcggccga acgaggcccg ccgggtcgac
     6421 gatgaggcca tcgtggacga gcttgcgacc gaggtctcgc cccagctgga cgtcttgcga
     6481 gcacggtatg ccgcggtgct gggtgatgcg ctcaagcggg cggtcgcagg cctgcgtccg
     6541 gaccagcgcg tcctcctgcg catgtacttc gtatcgggcc acagcacgca gcagatcgcg
     6601 acgttgttgc gcgtcgatcg atcgacggcg gcgaggcgac tggtcgcggc gcgacaggcg
     6661 gtgttcgatg agacccaccg ctggcttcgc accgagctgg cgatcgacag cagcgagttc
     6721 gccagcctcg ctcgcacgct tcacgaccag ctcaacgtga gcctgggtgg gctgctcgcc
     6781 gattcgccgg aaggctaatc aggcgacata tcgatgattt tggcctcgtt cgaaatcgga
     6841 gacgatttcg ctgcagcaaa cgccgtctcg gctgtccctt ggtcacaacg agacaaccag
     6901 aggacaagga gacgaacgat gaacacgatg tttcgacgtg ctggaatttt catgatcgcg
     6961 gcgatgacga ccgccgtcgc cgccgcggcg tgggcgcgat cgctgcccgc atccaccggg
     7021 caatcactcg gcccggagca ggcgacctgc ttccaccagt atggcaacct cggccaggtg
     7081 gagaatacgt gcagcttcac gaatcggttc tgcgtgccgc tcgtcgtcga tagcacctgg
     7141 catgacgtcg atgtcacggt gaccgcgccg gacatcaatc acaatgtgaa ttgcttcgcc
     7201 gaaacgttct atccagactc gtcgttcgcg atgtggagtg gctttcaagc ggcgagccgg
     7261 ttcggcgccg ggcagacgct ggtgttcacc ggcctcagcg tgccgggcgg cggctacatg
     7321 ctgtcgtgct gcgacgtcgc gccgaacgcc tgggtggact ccttcaatta ctcgaactga
     7381 gtgatcatgc gcccgaattt gatctggctg gggatcgtgt ccgtcgccgc ggtgggggtc
     7441 ggcggataca tcgtgcacaa ccaagcgctc gcggagctgc accgcgagat cgccgctgcc
     7501 cgcgcgacgg cggcggtcgc gtctcggcca ccacccgtcc cggtgatcgc ggcgcagacc
     7561 gccccggcgc tcgcgatgcc ctcgcatgcg ccggcacaac cgtccaccga ggaccaggct
     7621 tcgccgccgc ggaatccgga aggcggctcg cagagcgctt cacgctggaa ggaggaagcg
     7681 ctcgcgcagc gcacggcgat cgaagccggc ttcgccgcgg aggtcatcga tggcgcctgg
     7741 gccgcggcgg cgaggcgcga cctgcgcgat cgaatctcgg ctctcctgcc ttcgacctcg
     7801 tcgctcgccg acgtcgattg ccgcgcgtcg atgtgccgcg tcgagctcgt tcatcgcgag
     7861 gccgcgggct cgcagcggtt catgcaggcc gcattcttcg atccgaagga ccgggtgtgg
     7921 agtggccctg gcttcgccat cccggcggag accggcagcg acgggacggt gaccgtcgtg
     7981 ctctacctcg cgcgcgacgg tgcatcgttc ccgatgcccg atacgagatg aacgggcgca
     8041 ccaggacatg atcgacgact tcgccgccgc gctcggctga ccggcgcgtg gcgatgccgg
     8101 tcgcctggat aaggagctac ctccgccgcg ctgcttcgat ggcctttcgc gcccacacgc
     8161 ggtcgagctc cttgacctcc tcgggcaggt ccttgtaagg gacccagtac ttcatccagc
     8221 gggcccggcg tgcagggctc acctcgggtg ccacctcctt ggaccacgcc atccactgtg
     8281 cgtgctcgac ttccgccacc cgctcgacgg cctcccgctc gctgtcgcac gaacacaggg
     8341 cggccacgac actcaccatc actgccagca tcttcgctcg aacggacata ctgatctcct
     8401 ggttcccaga gcatgcagga tatcaggaac gcttcgacgt gcccggcttc ccgatgccga
     8461 acggtggaga atacccgata cgatgcgttc gcaccatgcc gtctcgcgtc gtccacattg
     8521 cctttgggtc tgacgccggt cagtcatgcc ggctcaggcg acaacttccc tgagacatgg
     8581 ggaggtcgag cgcgctggat cgggcgccgt ccacgcgcgc gtcgcgccgt tgccctgtgc
     8641 gcccgacggg ccgctcggca tcgtccgcgg cgcgacgctc gtcatgcact acgaggccga
     8701 tgtcttcccc ggcggcctca ccgtccgctc gcgcgatccc gatcccacga ccaccgcgta
     8761 cggcatgctg acggatctcg tgggccttct ccgatctgga cgcaattccg cgacctgatc
     8821 gcgcttctcc accggcaccc cctccgcgaa gagcgcaggg accgtgatgc gacgacgcga
     8881 gcgtcgcacc gcctgcccgg ggttgcagtg ccttgccgaa cccgcgtgac cccggatccg
     8941 gtacggtgtt tcgccatgac cgcgccgatc gatcacgtcg cgaagaaccg ggcgtactgg
     9001 gccacgctcg ccgccgagta cgcggcgacc gccgaccgcg cgtgggtggc ccgcgagccg
     9061 acctggggga tctgggaagt ccccgaggcc gagctcggcg tgttgcccgc ggacctgacc
     9121 gggctcgacg cgatcgagct cggctgcggc accggctacg tctcggcctg gctcgcgcgc
     9181 cgcggcgccc gcccggtcgg catcgacaac tcgcccgagc agctcgcgac cgcgcgccgg
     9241 atgcaggcca gccacggctt gacgttcccg ctgcacctcg gcaacgccga ggcgacgccg
     9301 ttcgacgacg ccagcttcga tctcgcgatc agcgagtacg gtgcctcgat ctggtgcgat
     9361 ccgtaccgct ggatccccga ggcatcgcgc ctgctgcgcc cgggcggcca gctgatcttc
     9421 ctgcgcaact cgacgctgct gatgctcacg gtgcccgagg ccaacggcgt cgccgcaacc
     9481 gaccggctgg tgcggccgct gcgcggcatg caccgcctca tctggcccga gccgtcgggc
     9541 gtggagttcc acatcagccc cggcgagctg atccggctgc tccgccgcac tggcttcgag
     9601 ctcgaggaca tgatcgaggt ctatgccccc gagggcggca cgacacggta tccgtttgtc
     9661 gacctgccct ggtcgcgcag ctggccgtgc gaggaggtct ggaaggcccg caagtggctg
     9721 tgacgtccga cgatggggcg gccgcggctg tgttacaccc gcgtggggag gccgcatgaa
     9781 gaagtaccgc tgctcgatct gcagtcacat ctacgacccc gccgagggcg acccggacac
     9841 cggcatcccg ccgggcacgc cgttcgagaa gctcccggac agctggtgct gtcccgactg
     9901 cggggcgagc aaggaagact tcgagccgat ggaggactga gggcgccgct cgttcgacgc
     9961 ccacgatcgc gggctgactc agctcaccga cgcctggacg aggtaatcga ccagacgctc
    10021 ggcgaagttc ggtcgcttcg ggatcaggga gagcaccaca cgcagcatcg catcgcgtga
    10081 ctcgaacggc aggccgcgct cgatcgcgtc gcacagctca tcgccccagt cgccgaggtc
    10141 ggcgtcctcg gcgatctcca ggatcgcgcg ccggaaatcg gagcgggtgt cgttgtagaa
    10201 gctgcccttc agggtctcga cctcgcgccg ggggcgaggt ggcggaggct gttcggtcca
    10261 ctcgatggag ctgaagttgg cggtgaggtc ggcgagatcg atccagaaca cgccgttgcc
    10321 ctcgaaggcg accggcttga gcttgccgtc gatcttctcg taggtgcgcc cgtagtgacc
    10381 ccatgggttg cgcagcttga tcaggatctg ctcgcccggc ttggggtcgg gccgcggctt
    10441 gtaatcgagc aggctgtacg cgtgcttgcc ggccaggccc ttcaccttcg actcgccgca
    10501 catccccttc ttgttctcct cctcgttctc gctgtcgctg atcgtatcgc gcgtcgacgc
    10561 gcacatcgac cggttgcggt cgatgccgga cttgatgcgc ttgaacgcct cgctctcgtc
    10621 ggtgccgtag cgcccgctgc cgatctcgcc cggcaagagc tgcatcgaat tcgcctgctg
    10681 cacgagctgc ttgagaggct cgttgttggg gtgcttgcgc accagcgcct ggaggctggt
    10741 gtcgctcacg aacaccagct tcaggcggtt cgacagatcc aggatcggct cgtccatctc
    10801 caccagctgc tggaccagcc cctcgatctc ctcgccgatc ctcttgaggc gcgactcgtc
    10861 ggtcgtcacg tcgacctgct gctcgcgctt gtgctcgatc tgcttcttca acagcgtctc
    10921 ttcatcctcg agctgttgga tctgcttctt gatgtgctcg ccggcctgct ggagcctgac
    10981 cggcgcttcg tggccaaacc ttgcgcggct cgtgccgagg tcgaacgacg acggcggctt
    11041 gccggtcagg tgtagcagcg cgtcgccggg gtcgccgccc gcgatgtcct ggtacgacgg
    11101 cggcgagtcg ttgcgcggtt cgttgttctt ccgcttcctg tccttgttct ccttctgcaa
    11161 ccccgacgac gcgtaggcct tctcgaggat ctgcacccac aggctgttgc gggcatacct
    11221 ggtggagccc tccccgatga cgatcgactt cgcgatcgcg atctcgaacg cttggccgac
    11281 gtccgcgtag agcttcaccg tcacggtgcc gtcgacattg tcgaccatgt tgcgaacgaa
    11341 gtggctggga tcgatctgca cgatgctggc cacggcggcg agcaggtagc agtcgccgag
    11401 gtagccctgg cggacgtcgt cgatatcggg gagggcagtg aacagcggcg cgtggtgggg
    11461 gtcctccatc agctcgtgcc tggaggtgtc gcggtgcttc tcgaggatct ccggatccga
    11521 ttgcatggtg aggctggggg cgaaccacgg ctcgtagtgg ccggtgctcg actggtacag
    11581 ctcgatcttc tcgcggtcga gctgcccgtt gatcgcggcg gcgagcgtgc gctgcttctc
    11641 ggtgaactcg ccctggtacg cgctgctcag cgacttgctg cgcgagtaga ccgtgtagaa
    11701 ggtggcgaac tggcgttcgt ggtcgttgtc gtcgatcttg aagtacggcg tgaccgcggt
    11761 gacgtacgcg gcccacgcgt cctgggtgat cgactgcaag aggctcggct tgaggagcgg
    11821 gttggcgccg aaccgcttca cgaagtcgga cggcttgcgc tggatcgcgg tgccgccccc
    11881 gctgcttccg gcatgctcgt cgctgcgacc ggcttgtaca tcaccaacgt ctcggtcagc
    11941 gtgcgcttgc cgggcgtgcc gaggcccgga tctctgcctc gacgatccgg atccgctccg
    12001 cccccagctc gtgagctctg agttcgcatg tcgagcgaaa cctatgaccg ccgcgactct
    12061 gactccatga ccgccgatta cggggtctta catcaggcga tgtagccccg ctgcaccgcc
    12121 gggatcgcta cgtgcgcgtc ctgcccgcag cagggcggcg gcggttgcga cggcggcgtg
    12181 accggggagc cggcgacgcc gagcgccttt ccatcttccg gcacgcgcca gaggtagctc
    12241 gcgcgccagg tcggcgcggt ctcctcagcg cgcgccatcg ccccgggtgc gctacgctcg
    12301 ccgtgccatg agcgcggcgc aacagatcct ggatcaagcc tgctcgctcg cgcggatcgc
    12361 ggtgctcgag gccgatcccg ccgtgtatgc cgagcggctc gccgccgagc tccccgcggt
    12421 ggccacgctc atcgggctcg cggcgcgcga cgcggctctg gtcgccgcgc tcggccagat
    12481 cgacgcgatg gtcgcgcgcg cgatgcgaat ccggctcgat cacgccctcg cgagcgacac
    12541 ctcgatcggc gcgccgaccc ggagcgtgtt cgcatcgacg atcacgggct acgccgggcg
    12601 gctgtcgctg ctggagcagc gagcgcgcga catcgctgtg cgcggccggg ccgccgaccc
    12661 cgacctcgtc gcgaagtccg tcgtcgaggc ggggtgcgcg gtcctggcgc tgcgcgatgc
    12721 gcttcgcgcc ggcgtgctcg cgctgatccg cgacctcgcc cgggaggccg tcaccgaggc
    12781 cgaccgccgg gcgcgcgatc gcagcctcga cgagtcagag cgacggcgct ggagcgcggc
    12841 tcgccgcgat ctcgaggccg tcgcggccga gcccgagcgc gtgctcaccg cgcccatggc
    12901 cgcgcgtctc ggcgcgctgc ccgaccagct cgacgagccc gggatggacc gcgaggttgc
    12961 gttcgccgac ctgctcgagc tcgactagcg agcgctactc gtcgacctgg ctcagctcga
    13021 tcaggtggcg gcgacgcgcg gacatcaggt atgcgaacct ctcgccacca tgccgatcct
    13081 cgaggtgacg atgagctact cccccctggc gttcctgtac gccttctcga cgccaaccat
    13141 cacgatcaac gagacccgtg acaggcggcc gtggggcacg tatcgattcg agctgccgcc
    13201 aggcaactat cgcgtcgaga tgtcgtatcc gtggctcatc agtccagagt gcggcaagaa
    13261 cagcgtcgat atcgtcttgc gtgaaggaga ccacaagcac gtgcgctaca cggcgcgcat
    13321 gctgcgttac tggcccggca gggtctcggt cgacgatgtg atcccgacgg cgcgcgcgct
    13381 gcgcgaccgg tcgacgtgac gtcagcgccg taccgccggc gcgacgccgc gaccgcagct
    13441 gcccccagtt ccgctaccga ctcgctaccg gacgacgcaa gccgtcgaga tagtggctac
    13501 ttcttcggct cggccttggt cgcggcagcc ggcgccggcg ctggagcctt cgtcacagcg
    13561 gccggcgccg gtgccggagc cttggcgggg gcggccgagg ccgccttggc aggagcggcg
    13621 cccgcggcgg gg
//
LOCUS       DASVOR010001050         1787 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390146,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001050 DASVOR010000000
VERSION     DASVOR010001050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390146"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1787)
                     /locus_tag="VF516_20230"
     CDS             complement(<1..>1787)
                     /locus_tag="VF516_20230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110074.1"
                     /translation="AEALEAARQLYHSLGDDAMVARLFGAELEVLGGEKSPAAAGRKA
                     QIRLELGKLALRGKDLEAAAGHLEEAARLDPTSLETAEKLAEVYATPGFRGGDDGAWR
                     HKASQLFVEVGRRRMASRDDTTGINYLRRAAGIDPYSKGSSVALEQALSEASQWQELD
                     RMLRHRSNVVQDPAERAEVLRRRAALYRNQLPDRDGLIEVLTELVAYEPPGSKAAREL
                     RELLRDDQEWEALTRLMEAEINALGADPNTPASALVDEILELATVAREHMGDRDRAAE
                     LLHQALQVDPTHEEALARYVDHFRERRDWRGLIDLMEFALDNVREAGAPDDELVRRLE
                     EIAQLAELRLGDIPRAIDAWQRIAQLEPGSPKVAEALRRLSARAKMWEQLVKTLEHEL
                     AAAPTAGQRNHVLKKMAQTYRERQIEPRRAIELYEQIVADNPDDDAVLKALGELYERE
                     GDDAGLAATLRRVLELEDRRIGEAMARAGKPADAPREWPVAKRAERLTLLRRLAVLYE
                     TRLADVDGVVWASSAVLELLHGDRDALERMERVLDKAGDPRLEQTLEYHAAASTSPAE
                     RAKLLKRLARLAAARTDEVAALERWEQTLR"
BASE COUNT          190 a          718 c          615 g          264 t
ORIGIN      
        1 cgcagcgtct gctcccagcg ctcgagcgcg gcgacctcgt cggtgcgcgc cgccgcgagc
       61 cgggccaggc gcttgagcag cttggcgcgc tcggccggcg aggtcgacgc cgccgcgtgg
      121 tactccagcg tctgctcgag ccgcgggtcg ccggccttgt cgagcacgcg ctccatgcgc
      181 tcgagcgcgt cgcggtcgcc gtgcaacagc tcgagcaccg cgctcgacgc ccacaccacg
      241 ccgtcgacgt cggcgagccg ggtctcgtac agcaccgcga gccggcgcag cagggtcagc
      301 cgctcggcgc gcttggcgac cggccactcc ctcggcgcat cggccggctt gcccgcgcgt
      361 gccatcgcct cgccgatccg ccggtcctcg agctccagca cgcggcgcaa ggtcgccgcc
      421 agcccggcgt cgtcgccctc gcgctcgtac agctcgccca gcgccttgag caccgcgtcg
      481 tcgtcggggt tgtcggcgac gatctgctcg tacagctcga tcgcgcggcg cggctcgatc
      541 tggcgctcgc ggtaggtctg cgccatcttc ttcagcacgt ggttgcgctg gccggcggtc
      601 ggcgcggcgg cgagctcgtg ctccagggtc ttgaccagct gctcccacat cttggcccgc
      661 gccgacagcc ggcgcagcgc ctcggcgacc ttggggctgc ccggctcgag ctgcgcgatc
      721 cgctgccagg cgtcgatcgc gcgcgggatg tcgccgagcc ggagctccgc gagctgcgcg
      781 atctcctcga gccggcgcac cagctcgtcg tccggcgccc cggcctcgcg cacgttgtcg
      841 agcgcgaact ccatcaggtc gatcagcccg cgccagtcgc ggcgctcgcg gaagtgatcg
      901 acgtagcgcg ccagcgcctc ctcgtgggtc gggtcgacct gcagcgcctg gtgcagcagc
      961 tccgccgcgc ggtcgcggtc gcccatgtgc tcgcgcgcga ccgtcgcgag ctcgaggatc
     1021 tcgtcgacca gcgcgctcgc cggcgtgttc gggtcggcgc cgagcgcgtt gatctcggcc
     1081 tccatcagcc gggtcagcgc ctcccactcc tggtcgtcgc gcagcagctc gcgcagctcg
     1141 cgcgcggcct tgctgcccgg cggctcgtac gccaccagct cggtcagcac ctcgatcagc
     1201 ccgtcgcgat cgggcagctg gttgcggtac agcgcggcgc gccggcgcag cacctccgcg
     1261 cgctcggccg gatcctgcac cacgttgctg cggtggcgca gcatgcgatc gagctcctgc
     1321 cactgcgacg cctccgacag cgcctgctcc agcgccaccg agctgccctt cgagtacgga
     1381 tcgatgcccg ccgcgcgccg caggtagttg atgccggtcg tgtcgtcgcg cgacgccatc
     1441 cgccgccggc cgacctcgac gaacagctgg ctcgccttgt ggcgccacgc cccgtcgtcg
     1501 ccgccgcgga acccgggcgt cgcgtagacc tcggccagct tctcggcggt ctcgagcgac
     1561 gtcggatcga gccgcgccgc ctcctcgagg tgccccgccg cggcctcgag gtccttgccg
     1621 cgcagcgcga gcttgccgag ctcgagccgg atctgcgcct tgcgccccgc cgccgccggc
     1681 gacttctcgc cgccgagcac ctcgagctcg gcgccgaaca gcctggccac catcgcgtcg
     1741 tcgccgagcg agtggtacag ctggcgcgcc gcctccagcg cctcggc
//
LOCUS       DASVOR010001051         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448722,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001051 DASVOR010000000
VERSION     DASVOR010001051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448722"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF516_20235"
     CDS             <1..492
                     /locus_tag="VF516_20235"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX8110075.1"
                     /translation="ANVAAALSRLAGATHRALAARIAKASGQGFERLIHAYLVAAGYR
                     DISWIKRVGGISYAQAIAPGLDRMVLISARSGDQPIDRRGIGELRVGVEAKDLVAGIL
                     FSPRELSEDAERELERAGRSISVICGDQLVAALISVGVGVVSAAAPLHYVDDQLLDEL
                     LAG"
     gene            503..1096
                     /gene="lipB"
                     /locus_tag="VF516_20240"
     CDS             503..1096
                     /gene="lipB"
                     /locus_tag="VF516_20240"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEX8110076.1"
                     /translation="MTTAWQWLGRAEFGRVLADQLAARERVWAGEPGVVLLCEHPPVI
                     TLGRSASVAHILAAGEIPVERIERGGEVTYHGPGQLMVYPIVRLRSVVGFLERVAAAI
                     AETCAVLGAPGAAWRRDPAGVWLDGKKLAACGIHVARAVSVHGFALNVDTPPEAWRAI
                     RPCGLDVPQISLVRARGAPITVAEVAQELGPRVAQAV"
     gene            1112..>1650
                     /locus_tag="VF516_20245"
     CDS             1112..>1650
                     /locus_tag="VF516_20245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110077.1"
                     /translation="MLQPLRIAVLYNTDYDAEACATDVISVEASARAVAAALTDGGFA
                     VELIGLYGLEVLAVIDRIRAAAPDLVFNLCESMAGNPRNEPTFVGLLDLFGIPYTGAD
                     LLCLASCLHKRRAKDVLLARGLPTPPYRFLVDAGALGDPALDALDYPWFVKLAHEDAS
                     VGITEENLVATPAALRKRAG"
BASE COUNT          190 a          599 c          617 g          244 t
ORIGIN      
        1 gccaacgtcg ccgcggcgct gtcgcggctc gccggcgcga cccaccgtgc gctcgccgcg
       61 cggatcgcca aggccagcgg ccagggcttc gagcggctga tccacgccta cctggtcgcc
      121 gcgggctatc gcgacatctc gtggatcaag cgggtcggcg gcatctccta cgcgcaggcg
      181 atcgcgccgg ggctcgaccg catggtgctg atcagcgcgc ggtcgggcga tcagccgatc
      241 gaccggcgcg ggatcggcga gctccgggtc ggggtcgagg ccaaggacct ggtcgccggc
      301 atcctgttct cgccgcgcga gctgtccgag gacgccgagc gcgagctcga gcgcgccggc
      361 cggtcgatct cggtgatctg cggcgaccag ctggtcgcgg cgctgatctc cgtcggggtc
      421 ggcgtggtca gcgcggcggc gccgctgcac tacgtcgacg accagctact cgacgagctg
      481 ctggccggct agccttgcgg gcatgaccac cgcctggcag tggctcggcc gcgccgagtt
      541 tggccgcgtg ctcgccgacc agctcgccgc ccgcgagcgg gtgtgggccg gggagcccgg
      601 cgtcgtgctg ctgtgcgagc acccgccggt gatcacgctg ggccgctcgg cgagcgtcgc
      661 ccacatcctc gccgccggcg agatcccggt cgagcggatc gagcgcggtg gcgaggtcac
      721 gtaccacggg ccgggccagc tcatggtgta tccgatcgtg cgcctgcgca gcgtcgtggg
      781 gtttctcgag cgcgttgctg cggcgatcgc cgagacctgc gccgtgctcg gcgccccggg
      841 ggcggcgtgg cggcgcgatc ccgccggggt ctggctcgac ggcaagaagc tcgcagcgtg
      901 cggcattcac gtcgcgcgcg cggtcagcgt gcacggcttc gcgctgaacg tcgatacgcc
      961 gcccgaggcg tggcgcgcga tccggccgtg cggcctcgac gtgccgcaga tctcgctggt
     1021 ccgcgcgcgg ggcgcgccga tcaccgtcgc cgaggtcgcc caggagctcg gcccccgcgt
     1081 cgcgcaggct gtgtaagatg ttcggtgcgc catgttgcag ccgctgcgca tcgccgtgct
     1141 gtacaacacc gactacgacg ccgaggcctg cgccaccgac gtcatctcgg tcgaggccag
     1201 cgcgcgcgcc gtcgccgccg cgctgaccga cggtgggttc gcggtcgagc tgatcggcct
     1261 gtacggcctc gaggtgctgg cggtgatcga ccggatccgc gcggcggccc ccgatctggt
     1321 gttcaacctg tgcgagtcga tggccggcaa tccgcgcaac gagccgacgt tcgtcgggct
     1381 gctcgacctg tttggcatcc cgtacaccgg cgccgacctg ctctgcctgg cgtcgtgcct
     1441 gcacaagcgg cgggccaagg acgtgctgct cgcgcgcggg ctgccgaccc cgccgtaccg
     1501 cttcctggtc gacgccggcg cgctcggcga tccggcgctc gacgcgctcg actacccgtg
     1561 gttcgtcaag ctcgcgcacg aggacgcctc ggtcgggatc accgaggaga acctggtcgc
     1621 gacgccggcg gcgctgcgca agcgcgccgg
//
LOCUS       DASVOR010001052         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_419032,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001052 DASVOR010000000
VERSION     DASVOR010001052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419032"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(349..>3621)
                     /locus_tag="VF516_20250"
     CDS             complement(349..>3621)
                     /locus_tag="VF516_20250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8110078.1"
                     /translation="LTAVVAAGLATGVISAIELDRAAFADALTTGSIRGVVKDKTSGD
                     AGVGATVVATSPALQGEQVVIADETGSYYITNLPPGMYTLTVYYNDTTYSRSNVLIQV
                     GKEAFVNLTVDTSASKGETIAITGSVPIVDQGSTKVGTTITSDFTNNVPVRRTFGEVM
                     GGAAGAQGDAYGISFAGATSAENTYVVEGLNTTDTGFGTLSTNLPTEFIQETEVITGG
                     YNAEYGRALGAIVNVVTKSGSNEFHGSVFGHLQTSGLTANFKTILREGTAIDSATNQD
                     SRYDFGAELGGPIIKDKLWFHVGFTPTIINDTTTRIINRNVDMNQDGVADVDPNTGFT
                     VRQFVTSRDIPTSFKTYFFTGKLTGAISQNHQWELSAFGNPETDSALPLTTAGTPFVV
                     RDPNTSLLNVDRGAYDFSGKWTSKFNDGKTQFDAVAGFHRGYDNASPGSPGANLAGVQ
                     YKYTRSLYDFADIENSGKSNINGCHDSANDMPTADDPYPKITNCPVLNYIEQGAGFLE
                     KRTNDRISAVLAVTQRVKALGQHVFKAGIDAEFSSYNSGRGYTGSAYIVRDEPDQVQN
                     GMPLASEFTTQQFIRYDPNGTIPCGIDNKAKCSVIPQLNADTNDRSLAAYIQDQWQIL
                     PNLTLNAGLRWEQQVGYVAGFLQNTIAPDTGEKIPEVAFNLNNLIAPRVGFIYDPTQE
                     GKAKLMGHWGRFYENIPMDMNVRAFGGETRINEIVGVDSSGSLNNSCMFDHGAPNLIS
                     GVLACNPGRSQILGGGITYFAPGTQGQYTEEFILGGEYEIIPDITVGANYIHRSLPRV
                     IEDMSTDGGNNYFIANPSENYDSQAADLDKLAMNTTDPNLKSLYQSRANQLRSVKNFD
                     PPSRNYDALQLVARKRPTGNSLLQATYTYSRAKGNYPGLFSTETNQLDPNITSQYDFP
                     NLMPNRYGPSGLDRPHSIKVDGFYLFNFNKAGLLVTGVSFRGVSGVPANTLAAHFIYG
                     PDESYLLPRGALYRSPFTTEVDTLVSYGYQLNKTTRLEGFVDIFNLLNSQDELSVDGT
                     YTTGSAIPIVGGDRNDLAHAKTTTDEGRQVGTTLTKNLNYGKLNAHQNPLTGQLGFRL
                     TF"
BASE COUNT          542 a         1095 c         1281 g          703 t
ORIGIN      
        1 aggatgatgg tgtcgaccgg gtgctcgttc ccgtcggcgt cgaggatgcc gccggccagc
       61 ggcgccagcg gggtcaacgg atggagcgcg gctccaaaca gcagcgcgct gccgaccatg
      121 ccggcggcag cgcgctgaaa cgacgagtga tcggcgaacc ggatgtccat gccgcctacc
      181 aacgctccgt gaacaaccat cgatctaaac tcgccccgaa acccgctggc cgtcggaccg
      241 cgcggcctaa ccggtcgtga tcacgagccg aaaacgaccg tggcctcctg ggcgggaggc
      301 cacgattcca ggggctccgc cgtgggcggg gccgcggtca atgcccggtc agaacgtcaa
      361 ccggaacccg agctgcccgg tcagcgggtt ctggtgcgcg ttgagcttgc cgtagttcag
      421 gttcttggtg agggtcgtcc cgacctgacg gccctcgtcg gtcgtggtct tcgcgtgcgc
      481 caggtcgttg cgatcgccgc cgacgatcgg gatcgcgctg ccggtggtgt aggtgccgtc
      541 gaccgacagc tcgtcctgcg agttgagcag gttgaagatg tcgacgaagc cctccagccg
      601 cgtcgtcttg ttcagctggt agccgtacga gacgagggtg tcgacctcgg tcgtgaacgg
      661 cgagcggtac agcgcgccgc gcggcagcag gtacgactcg tccgggccgt agatgaagtg
      721 ggccgccagc gtgttggccg ggacgcccga cacccctcgg aagctgacgc cggtgaccag
      781 cagaccggcc ttgttgaagt tgaacaggta gaagccatcg accttgatgc tgtgcggccg
      841 atcgagaccg ctcggaccgt agcggttcgg catcaggttc gggaagtcgt actgcgacgt
      901 gatgttcgga tcgagctggt tggtctcggt cgagaacagc cccgggtagt tgcccttggc
      961 ccgcgagtac gtgtacgtgg cctggagcag cgagttgccg gtcggccgct tcctcgcgac
     1021 cagctgcagc gcatcgtagt tgcgcgacgg cgggtcgaag ttcttgaccg accgcagctg
     1081 gttcgcgcgc gactggtaga gcgacttgag gttcgggtcc gtcgtgttca tcgccagctt
     1141 gtcgagatcg gcggcctggc tgtcgtagtt ctccgacggg ttggcgatga agtagttgtt
     1201 accgccgtcg gtcgacatgt cctcgatcac gcgcggcagc gagcgatgga tgtagttcgc
     1261 accgaccgtg atgtcgggga tgatctcgta ctcgccgccg aggatgaact cctcggtgta
     1321 ctggccctgc gtccccggcg cgaagtacgt gataccgccg ccgaggatct gcgaccggcc
     1381 ggggttgcac gccaggacgc cggagatcag attcggtgcc ccgtggtcga acatgcagct
     1441 gttgttgaga gagccgctgg agtcgacccc gacgatctcg ttgatccggg tctcgccgcc
     1501 gaacgcccgc acgttcatgt ccatcgggat gttctcgtag aaccgtcccc agtggcccat
     1561 cagcttggcc ttgccttcct gggtcgggtc gtagatgaag ccgacgcgcg gcgcgatcag
     1621 gttgttgagg ttgaaggcga cctcggggat cttctcgccg gtgtccgggg cgatcgtgtt
     1681 ctggaggaag ccggcgacgt acccgacctg ctgctcccag cgcagacccg cgttgagcgt
     1741 caggttgggc aggatctgcc actggtcctg gatgtacgcg gcgagcgagc ggtcgttggt
     1801 gtccgcgttg agctggggga tcacgctgca cttggccttg ttgtcgatgc cgcaggggat
     1861 cgtcccgttg gggtcgtacc ggatgaactg ctgggtcgtg aactcgctgg ccagcggcat
     1921 gccgttctgg acctgatcgg gctcgtcccg gacgatgtac gcagagcccg tgtagccgcg
     1981 gccggagttg tagctcgaga actcggcatc gatgccggcc ttgaacacgt gctggccgag
     2041 tgccttgacc cgctgggtca ccgccagcac cgccgagatc cggtcgttgg tccgcttctc
     2101 caggaagccg gcgccctgct cgatgtagtt caggaccggg cagttcgtga tcttcgggta
     2161 cggatcatcg gcggtcggca tgtcattggc gctgtcgtgg cagccgttga tgttcgactt
     2221 gccactgttc tcgatatcgg cgaagtcgta cagcgatcgc gtgtacttgt actggacccc
     2281 ggcgaggttg gccccggggc tgcccgggga cgcgttgtcg tagccgcggt ggaagccggc
     2341 cacggcatcg aactgggtct tgccgtcgtt gaacttcgag gtccacttgc ccgagaagtc
     2401 gtaggcgccc cggtcgacgt tcaacagcga cgtgttcgga tcgcgaacca cgaagggggt
     2461 tccggcggta gtcaacggca gcgcggagtc ggtctcgggg ttgccgaacg cggagagctc
     2521 ccactggtga ttctgcgaga tcgcgccggt cagcttgccg gtgaagaagt aggtcttgaa
     2581 cgacgtcggg atgtcgcgcg aggtgacgaa ctggcgcacc gtgaacccgg tgttgggatc
     2641 gacgtcggcg acgccatcct ggttcatgtc gacgttgcgg ttgatgatgc gcgtggtggt
     2701 gtcgttgatg atcgtcggcg tgaacccgac gtggaaccac agcttgtcct tgatgatcgg
     2761 accgccgagc tcggcgccga agtcgtagcg gctgtcctgg ttcgtggcgc tgtcgatcgc
     2821 cgtgccctcg cgcaggatgg tcttgaagtt ggccgtcagc ccggaggtct ggaggtggcc
     2881 gaacaccgag ccgtggaact cgttggagcc cgacttggtg accacgttga cgatcgcgcc
     2941 gagcgcgcgg ccgtactcgg cgttgtaccc gccggtgatc acctcggtct cctggatgaa
     3001 ctcggtgggg aggttcgtcg acagcgtgcc gaacccggtg tccgtcgtgt tgaggccctc
     3061 gaccacgtag gtgttctccg ccgaggtcgc gccggcgaac gagatgccgt aggcgtcgcc
     3121 ctgcgcgccg gccgcgcctc ccatcacctc gccgaaggtg cgccggaccg ggacgttatt
     3181 ggtgaagtcc gaggtaatgg tggtgccgac cttggtcgag ccctgatcga cgatcggcac
     3241 gcttccggtg atcgcgatcg tctcgccctt ggacgccgag gtgtcgacgg tgaggttgac
     3301 gaacgcctcc ttgccgacct ggatcagcac gttgctccgg gagtacgtcg tgtcgttgta
     3361 gtacaccgtg agcgtgtaca tgcccggcgg caggttcgtg atgtagtagc tgccggtctc
     3421 gtcggcgatc acgacctgct caccctggag cgctggcgag gtggcgacca ccgtcgcgcc
     3481 cacgccggcg tcgccggagg tcttgtcctt gacgacgccc cggatgcttc cggtggtgag
     3541 cgcatcggcg aacgcggcgc ggtccagctc gatcgcggag atcactccgg tcgcgagccc
     3601 tgcggcgacg actgctgtga g
//
LOCUS       DASVOR010001053         5072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_156741,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001053 DASVOR010000000
VERSION     DASVOR010001053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5072
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156741"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VF516_20255"
     CDS             complement(<1..1078)
                     /locus_tag="VF516_20255"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX8110079.1"
                     /translation="MPERLYIVDGHGYIFRAHYGLMNSSRGERREVRLSTSEGMPTGA
                     LYVFSRMLMRLEEDFAPRRMAVVFDDKTGVRTFRARMYPAYKATRKEPPEELQVQMDY
                     FPKVVQGMGWPVLAVPGVEADDVIATLVRQARERDWEVVIFSADKDIMQMVGDHVSMI
                     DALHQKTYTREEVIKKMGVPPEKIPDFLALVGDTSDNVPGIKGVGDKTAANLLEQYGT
                     LDNLIAQNPVVPRISIKQPFGDPEQLERIKISRQLVELRRDVPLPVPLDDLVVGEWDL
                     PGLHQLFTELEFQVLVDKVKIRMPPADDVVVVPAPEVHQVASVDQAPDARIVVRGDAI
                     AELVAAARKAGRMAVTVELAPERPE"
     gene            1422..2498
                     /gene="plsX"
                     /locus_tag="VF516_20260"
     CDS             1422..2498
                     /gene="plsX"
                     /locus_tag="VF516_20260"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEX8110080.1"
                     /translation="MHRIVVDAMGGDHAPEEIVHGAAEASLALTSAEIILVGDAAELG
                     RLLPRMRHDGARVRVHHAPTFIEMDEKPAEALAAKPEASIAIAADLVARGEGDALVSA
                     GNTGASVLACARRWTLLQGVRRAALAAVYPTELRRGEKDDPFSLILDVGATVDATAED
                     LVGFAVMGSAYAKLVSQNRRPRVALLSNGTEPVKGPASVVAAHAALVETTELNFIGNI
                     EGLDIPRGVADVVVTSGFVGNVVLKMLEGVSETVVRLARYAHKERLAWRLGLIALSSA
                     IDQLKQITDWQQYGGAPLLGFTHPFIKAHGRSNAHAVTNAIKVAHKALAGNLCGNIAR
                     TMAELDERASRRPRQTDAGESGAP"
     gene            2495..3640
                     /locus_tag="VF516_20265"
     CDS             2495..3640
                     /locus_tag="VF516_20265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008195 - phosphatidate phosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase domain-containing protein"
                     /protein_id="HEX8110081.1"
                     /translation="MSPRTVLGRLGRMATLPSRVGQRLGLLDHIPEGLPPGASPPRHT
                     FRWDLDKTYLRTEFDSLGDLVRSAIETAADKQAFPGATALLRALRQDGHRICIVSGSP
                     TQMRQVLAAKLALDGVDYDEFVLKNNLKNFLRGRFRSLRAQIPYKLPAMLQSRIGAPA
                     DHETLFGDDAEADAIIYCLYADLVAGRVSLQDLERVLVASRAYDDDAIHILDLARRVP
                     KHDAVNRLFIHLDRRSAPVGFRHYGPRLVPVFNYFQAALVLYGDHVLSARQVIFVALE
                     MLDSGQFEIGHLATSVQDVIRRGRITREVALQLAAEAHDAARSGALSGRNDLPPFEQI
                     AEAFRDRVRELGPTEHYDFEPTAPLDYVRLVDQEHEHRRNRRRRGVQ"
     gene            3829..>5072
                     /locus_tag="VF516_20270"
     CDS             3829..>5072
                     /locus_tag="VF516_20270"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8110082.1"
                     /translation="MLVVEDDPAMRDLLTEELSDAGFDVEAAPTAAAGLELARVTRFD
                     LIITDLRMPEMDGFDLIRGVMQLPEPPHVIMITAFASIETAIRAVKLGAYDYIAKPFE
                     IEELLLVADKALGERALRNKVARLQREVEERFGLANIIAKSSAMRDVVALVQRIAGST
                     ASVLITGESGTGKELIARAIHYNSPRASGPFVGVNLAAVPEGLIESELFGHKRGAFTD
                     ARADKPGLFVEADAGTIFLDEIGELALPLQAKLLRVLQEHEVRPLGATRNQRVDVRIV
                     AATNRNLETMLADGSFREDLYYRLNVIHLDLPPLRARPEDIVPLAQHVLAQLGARQTP
                     PRRMRLSPEAQHLLLAYHWPGNVRELMNVLERGVALCQGELIADDDLPPHVREKRPAD
                     FLGAAVARRMTLAELEREFIERV"
BASE COUNT          726 a         1931 c         1700 g          715 t
ORIGIN      
        1 gctcggggcg ctcgggcgcg agctcgacgg tgaccgccat ccggccggcc ttgcgcgccg
       61 cggcgaccag ctcggcgatc gcgtcgccgc gcacgacgat ccgcgcgtcg ggcgcctggt
      121 cgacgctcgc gacctggtgg acctcgggcg ccggcaccac gaccacgtcg tcggccggcg
      181 gcatccggat cttgaccttg tcgaccagga cctggaactc gagctcggtg aacagctggt
      241 gcagccccgg caggtcccac tcgccgacga cgaggtcgtc gagcggcacc ggcagcggca
      301 cgtcgcggcg cagctcgacc agctggcgcg agatcttgat ccgctcgagc tgctcgggat
      361 cgccgaacgg ctgcttgatc gagatccgcg gcaccaccgg gttctgggcg atcaggttgt
      421 cgagcgtgcc gtactgctcg agcaggttcg ccgcggtctt gtcgccgacg cccttgatcc
      481 ccgggacgtt gtcgctggtg tcgccgacca gcgcgaggaa gtcggggatc ttctcgggcg
      541 ggacgcccat cttcttgatc acctcctcgc gcgtgtacgt cttctggtgg agcgcgtcga
      601 tcatcgacac gtggtcgccg accatctgca tgatgtcctt gtcggcggag aagatcacga
      661 cctcccagtc gcgctcgcgc gcctggcgga ccagcgtggc gatcacgtcg tcggcctcga
      721 ccccgggcac cgcgagcacc ggccagccca tgccctggac gaccttgggg aagtagtcca
      781 tctggacctg gagctcctcg ggcggctcct tgcgggtcgc cttgtacgcc gggtacatcc
      841 gggcgcggaa cgtgcgcacg ccggtcttgt cgtcgaacac cacggccatc cggcgcggcg
      901 cgaagtcctc ctcgagccgc atcagcatgc gggagaagac gtagagcgcg ccggtcggca
      961 tgccttcgct ggtcgacagc cggacctcgc gccgctcgcc gcgcgagctg ttcatgagcc
     1021 cgtagtgggc gcggaagata tatccgtgcc cgtcgacgat gtagaggcgc tccggcaccg
     1081 gcgcactcta gcgcggaaac ccggcatcga tcgccgcgtc ccgccgcgtc cgccccgctg
     1141 gtcggcgaga tcgggtcgtc agcgccttcc gccggagcgc gcggccggcc cgaccgtcag
     1201 gagccgcggc gcgcgaagcg accgccgagc gtctcgccct gccgggttgc tccgggggcc
     1261 gtgtacggca acggaggctg gcgagccccg ggtgagactc gggacggaac cgcgcgtcca
     1321 cgtcacagcg ctgaatcggc catcaggcaa gttccgcgaa gggtgagatc accggccgcc
     1381 cgccggctcc gaacagttct tgcctgtggc aagatgcgga tatgcaccgc atcgtcgtcg
     1441 acgccatggg aggcgatcac gcccccgagg agatcgtgca cggtgccgcc gaggcctcgc
     1501 tcgcgctgac ctcggccgag atcatcctgg tcggcgatgc ggcagagctc ggccgcctgc
     1561 tgccccgcat gcggcacgac ggcgcccggg tgcgggtcca ccacgccccg accttcatcg
     1621 agatggacga gaagccggcc gaggcgctcg ccgccaagcc cgaggcctcg atcgcgatcg
     1681 ccgccgacct cgtggcgcgc ggcgagggcg acgcgctggt gtcggccggc aacaccggcg
     1741 cgagcgtgct ggcgtgcgcg cggcgctgga ccctgctcca gggcgtgcgg cgggccgcgc
     1801 tcgcggcggt ctaccccacc gagctccggc gcggcgagaa ggacgatccg ttctcgctga
     1861 tcctcgacgt cggcgccacc gtcgacgcca ccgccgagga cctggtcggg ttcgccgtca
     1921 tgggctcggc ctacgccaag ctcgtgtcgc agaaccgccg gccgcgggtc gcgctcctgt
     1981 ccaacggcac cgagcccgtc aagggcccgg cgtcggtcgt cgccgcgcac gccgcgctgg
     2041 tcgagaccac cgagctcaac ttcatcggca acatcgaggg cctcgacatc ccgcgcggcg
     2101 tcgccgacgt cgtcgtcacc tcggggttcg tcggaaacgt cgtgctcaag atgctcgagg
     2161 gcgtgtccga gaccgtggtc cggctcgccc gctacgccca caaggagcgc ctggcctggc
     2221 gcctgggcct gatcgcgcta tcgtcggcca tcgatcagct caagcagatc accgactggc
     2281 agcagtacgg aggcgctccg ctgctcggct tcacccatcc gttcatcaag gcgcatggcc
     2341 ggtccaacgc gcacgccgtg accaacgcga tcaaggtcgc ccacaaggcg ctcgccggca
     2401 acctgtgcgg caacatcgcg cgcaccatgg ccgagctcga cgagcgggcc agccgccggc
     2461 cccggcagac cgacgcgggc gaatccggcg ccccatgagc ccgcgcaccg tgctgggccg
     2521 cctggggcgc atggccacgc tgccgagccg ggtcggccag cgcctcggcc tgctcgatca
     2581 catccccgag ggcctgccgc ccggagccag cccgccgcgc cacaccttcc gctgggacct
     2641 cgacaagacc tacctgcgca ccgagttcga ctcgctcggc gatctcgtgc gcagcgcgat
     2701 cgagaccgcc gccgacaagc aggcgttccc cggcgccacc gccctgctcc gcgcgctgcg
     2761 ccaggacggc caccggatct gcatcgtgtc cggcagcccg acccagatgc gccaggtgct
     2821 cgccgccaag ctcgcgctcg acggcgtcga ctacgacgag ttcgtcctca agaacaacct
     2881 caagaacttc ctgcgcggcc ggttccgctc gctgcgcgcc cagatcccct acaagctgcc
     2941 ggcgatgctg cagtcgcgga tcggcgcccc ggccgatcac gagaccctgt tcggcgacga
     3001 cgccgaggcc gacgccatca tctactgcct gtacgccgac ctggtcgctg gccgggtctc
     3061 gctgcaggac ctcgagcgcg tgctggtcgc ctcgcgcgcc tacgacgacg acgcgatcca
     3121 catcctcgac ctcgcccgcc gcgtccccaa gcacgacgcg gtcaaccggc tgttcatcca
     3181 cctcgatcgc cgctcggcgc cggtcggctt ccgccactac ggcccgcgcc tggtcccggt
     3241 gttcaactac ttccaggccg ccctcgtgct ctacggcgac cacgtgctgt cggcccgcca
     3301 ggtcatcttc gtcgcgctcg agatgctcga ctccggccag ttcgagatcg gccacctcgc
     3361 gaccagcgtc caggacgtga tccgccgcgg ccggatcacc cgcgaggtcg cgctccagct
     3421 cgccgccgag gcccacgacg ccgcgcgctc cggcgcgctg tccggccgca acgacctgcc
     3481 gccgttcgaa cagatcgccg aggcgttccg cgaccgcgtc cgcgagctcg ggccgaccga
     3541 gcactacgac ttcgagccga ccgcgccgct cgactacgtc cggctggtcg accaggagca
     3601 cgagcaccgc cgcaaccgcc gccgccgtgg cgtgcagtga gccgcgatac ccgcggtcgc
     3661 gattcgccac cggcatgtgg tgaatcgcag gaaaatcgcc ggttagctgc aacgccgccg
     3721 gccgggcgct gtctcgatcg cgtggccgcc ctgcccgccc tgcgcaagcc ttcccctccg
     3781 ccggccatgc tagaaccagc ggacatgccc caggagcgct cgggatccgt gctcgtcgtc
     3841 gaagacgatc cggcgatgcg cgacctcttg accgaggagc tgtccgacgc cgggttcgac
     3901 gtcgaggccg cgccgaccgc cgccgcgggc ctggagctgg cgcgggtcac ccggttcgat
     3961 ctgatcatca ccgacctccg gatgccggag atggatggct tcgacctgat ccgcggcgtg
     4021 atgcagctgc ccgagccgcc tcacgtcatc atgatcaccg cgttcgcgtc gatcgagacc
     4081 gcgatccgcg cggtcaagct gggcgcctac gactacatcg ccaagccgtt cgagatcgag
     4141 gagctgctgc tggtggccga caaggcgctc ggcgagcgcg ccctgcgcaa caaggtcgcc
     4201 cggctgcagc gcgaggtcga ggagcggttc ggcctggcca acatcatcgc caagagcagc
     4261 gcgatgcgcg acgtcgtcgc cctggtccag cggatcgccg ggtcgaccgc gtcggtgctg
     4321 atcaccggcg agagcggcac cggcaaggag ctgatcgcgc gcgcgatcca ttacaactcg
     4381 ccgcgcgcgt cgggcccgtt cgtcggcgtc aacctcgccg cggtgcccga gggcctgatc
     4441 gagagcgagc tgttcggcca caagcgcggc gcgttcaccg acgcgcgcgc cgacaagccc
     4501 ggcctgttcg tcgaggccga cgccggcacg atcttcctcg acgagatcgg cgagctcgcg
     4561 ctgccgctgc aggccaagct gctgcgcgtg ctgcaggagc acgaggtccg cccgctcggc
     4621 gccacgcgca accagcgggt cgacgtccgg atcgtcgcgg cgaccaaccg caacctcgag
     4681 accatgctcg ccgacggcag cttccgcgag gacctgtact accggctcaa cgtaatccac
     4741 ctcgacctgc cgccgctgcg cgcccggccc gaggacatcg tcccgctcgc ccagcacgtg
     4801 ctcgcgcagc tcggcgcgcg ccagaccccg ccgcgccgga tgcggctgtc gcccgaggcc
     4861 cagcacctgc tgctggccta ccactggccg ggcaacgtgc gcgagctgat gaacgtcctc
     4921 gagcgcggcg tcgcgctgtg ccagggcgag ctgatcgccg acgacgacct gccgccgcac
     4981 gtccgcgaga agcgcccggc cgacttcctc ggcgccgcgg tcgcgcgccg gatgaccctg
     5041 gccgagctcg agcgcgagtt catcgagcgc gt
//
LOCUS       DASVOR010001054         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_507208,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001054 DASVOR010000000
VERSION     DASVOR010001054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507208"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VF516_20275"
     CDS             complement(<1..886)
                     /locus_tag="VF516_20275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110083.1"
                     /translation="MVSTAGVGLPPAPQGDDVLRPGSKAGPWLVERELGRGGMGAVYA
                     VVHEEIGKRAALKVVHRRLMPGFNCERMLLEAKVVNQVGHPNIVDIFETGRFEDGRPY
                     IVMERLCGQSLAVCADDGKLLPDAVIAILLQVCDALIAAHAAGIVHRDLKLDNVFLVD
                     NFDDASQPRVKLLDWGIAKVIHTEVKHTIEGQLMGTPQYLSPEQARGLAVSPQTDVYS
                     LGVMAYELFLEQLPFEAETAAEVMALHLRATPPPPRELWPDIPAELEALLLAMLAKQP
                     DERPTMAEVARQLEAVRGE"
     gene            1056..>1816
                     /locus_tag="VF516_20280"
     CDS             1056..>1816
                     /locus_tag="VF516_20280"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HEX8110084.1"
                     /translation="MTREDLEASLAALRRQVADPRVGILGPRSIAWRIGGDVAVFLGG
                     GRAALLQLAHPMVAYAIAHHSRTRADVAGRFRRTFRSVFAMVFGDLDDAFRAARHVHA
                     IHAAIHGTIPEAVGGWAAGTPYCGNDAEALRWVHATLVDTTLVVREQLDGALPDAVKD
                     GYVIEMNRFAALFGIPEAMLPRSHAAHAAYMRQMLGSDRIAVAPCAREMAHFLVGRDA
                     EHQPSLGRIAEAVTALLLPPPLAAAFALRATPRRAR"
BASE COUNT          229 a          655 c          662 g          270 t
ORIGIN      
        1 gctcgccgcg caccgcctcg agctggcgcg cgacctcggc catcgtcggc cgctcgtcgg
       61 gctgcttggc gagcatcgcg agcagcaggg cctcgagctc ggccgggatg tcgggccaca
      121 gctcgcgcgg cggcggcggc gtggcccgca ggtggagcgc catgacctcg gccgcggtct
      181 cggcctcgaa cggcagctgc tcgaggaaca gctcgtaggc catcacgccg agcgagtaca
      241 cgtcggtctg cggcgacacc gccaggccgc gcgcctgctc gggcgacagg tactgcggcg
      301 tgcccatcag ctggccctcg atcgtgtgct tgacctcggt gtggatgacc ttggcgatcc
      361 cccagtcgag cagcttgacg cggggctgcg acgcgtcgtc gaagttgtcg accaggaaca
      421 cgttgtcgag cttgagatcg cggtggacga tgccggcggc gtgggcggcg atcagcgcgt
      481 cgcagacctg gagcaggatc gcgatcaccg cgtcgggcaa cagcttgccg tcgtcggcgc
      541 agaccgcgag cgactggccg cacagccgct ccatcacgat gtacggccgg ccgtcctcga
      601 accgcccggt ctcgaagatg tcgacgatgt tggggtggcc gacctggttg acgaccttgg
      661 cctccagcag catgcgctcg cagttgaagc ccggcatcag ccggcgatgt acgaccttga
      721 gcgcggcgcg cttaccgatc tcctcgtgta ccaccgcgta caccgcgccc ataccgccgc
      781 gcccgagctc gcgctcgacg agccagggtc cggccttcga ccccggtcgc agcacgtcgt
      841 cgccctgggg ggcgggtggc aacccgacgc cggcggtgct gaccatggtc cgcatatccg
      901 cattgccgtc gtgcacccgt tgtgcccgtg gccggccggg gcgaactgca tacttcgggg
      961 cagccgacgt ggcgggctcg cgcgctgtgg ccgttccgca cgccggacgt tgggcttgac
     1021 cggcagcgca acggcggtga ccatgatcca tgatcgtgac gcgcgaggat ctggaggcct
     1081 cgctcgccgc gctgcgccgg caggtcgccg acccgcgcgt cgggatcctc ggcccgcgct
     1141 cgatcgcatg gcggatcggc ggcgacgtcg cggtgttcct cggcggtggg cgcgccgcgt
     1201 tgcttcagct cgcgcacccg atggtcgcgt acgcgatcgc gcaccattcg cgcacgcgcg
     1261 cggatgtcgc agggcggttc cggcgcacgt ttcgaagcgt gttcgccatg gtgttcggcg
     1321 atctcgacga cgcgttccgg gccgcgcgcc acgtccacgc gatccacgcg gcgatccacg
     1381 gcacgatccc cgaggcggtg ggcggctggg ccgcggggac gccgtattgc ggcaacgacg
     1441 ccgaggcgct gcgctgggtc cacgcgacgc tggtcgacac cacgctggtc gtgcgcgagc
     1501 agctcgacgg cgcgctgccc gacgcggtca aggacggcta cgtgatcgag atgaaccggt
     1561 tcgccgcgct gttcggcatc cccgaggcga tgctcccgcg ctcgcacgct gcgcacgcgg
     1621 cgtacatgcg gcagatgctg ggctcggacc ggatcgccgt cgcgccgtgc gcccgcgaga
     1681 tggcgcactt cctggtcggc cgcgacgccg agcaccagcc gtcgctcggc cggatcgccg
     1741 aggcggtcac cgcgctgctg ctgccgccgc cgctcgccgc cgcgttcgcg ctgcgggcga
     1801 cgccgcggcg ggcccg
//
LOCUS       DASVOR010001055         2269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_215277,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001055 DASVOR010000000
VERSION     DASVOR010001055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215277"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..732)
                     /locus_tag="VF516_20285"
     CDS             complement(181..732)
                     /locus_tag="VF516_20285"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEX8110085.1"
                     /translation="MEVSMRARSIALLVCLAGCPRPNPPPGPGGGPPNVPPELAVPAG
                     NQVTVSAAAKGVQIYECAPDATGTPAWTLHAPRAELFDDAGNQVAVHFGGVDKGLPAG
                     PYWQANDGSRVHGAHPVSVANPGSIPLLRLDAADTSGTGIFSKVTFIQRLDTTGGVSP
                     TGACRATDTQEIAYTAKYYFYAK"
     gene            888..>2269
                     /locus_tag="VF516_20290"
     CDS             888..>2269
                     /locus_tag="VF516_20290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8110086.1"
                     /translation="MAFVPLVPIGDPLTKLVDDRTAGEQRSALGALEARLRDRRREVE
                     AGWGPTYIDRVHKKGKLTARERLARLADPDTRCYEVGTFVNHGEVFPGDQRSPGAGIV
                     TAFARVAGRWCMVIANDNTVASGAWWPRTPEKIQRAQTMALRLRLPTIYLVDCSGLFL
                     PEQSKSFPGARGAGHIFKMNSLLSAHGVPQIAGVFGDCIAGGGYMPIISDRVYMTEQA
                     YMVIAGAALIKGAKSQKITSLDIGGPEIHVHASGCADVRVPDDETLLACIRRDVARLP
                     SSGADFYRGELAALDPRFAAGELAGLLPVDHREAYDATQVLARLVDQSLFWEVMPEVG
                     EEMICGVGRVAGLYAGFVINRQGLVGDPDDPSRQRPGGILYRAGIAKISAFSRACNDD
                     GLPLIWLQDISGFDIGHQAEAHGLLAYGSSLIYTNSTNTTPMFTVLLRKASGAGYYAM
                     AGLPYDPVVQL"
BASE COUNT          311 a          823 c          804 g          331 t
ORIGIN      
        1 gcccgcggcg tcgacgacga gacggcgatc gcgttcatga tcggcggcgc cgcgctgggc
       61 gccacggtca tgttcgacct gacgcggacc cgccgtaaac cgtcgactgc gctccggctc
      121 gtcccgctga tcagcggcgg cggcgttcag ctcgcgctcg tcggtgcgcg ctgaccgcca
      181 ctacttggcg tagaagtagt acttcgccgt gtaggcgatc tcctgggtgt ccgtcgcgcg
      241 acaggcgccg gtcggcgaga ccccgcccgt cgtgtcgagg cgctggatga acgtcacctt
      301 ggagaagatg cccgtgcccg acgtgtcggc cgcgtcgagc cgcagcagcg ggatcgagcc
      361 cgggttcgcc acgctgaccg ggtgggcgcc gtggacccgg ctgccgtcgt tggcctgcca
      421 gtacggcccc gccggcaggc ccttgtcgac gccgccgaag tggacggcga cctggttgcc
      481 ggcgtcgtcg aacagctcgg cccgcggcgc gtgcagcgtc cacgccggcg tgccggtcgc
      541 gtcgggcgcg cactcgtaga tctgcacgcc cttggcggcg gccgaaaccg tcacctggtt
      601 gccggcgggc accgccagct cgggcggaac gttcggcggc ccacccccgg gtcccggcgg
      661 cgggttcggc ctgggacacc cggcgagaca cacaagcaac gcgatcgatc gagctcgcat
      721 cgagacctcc atcctgagcg cagcctacac gagcaggtgg acgccgtgcg ccttcatcgc
      781 ccaggagctt cgccgacgac gccgaccacc cggtcggaac gccatcgccc gacggatccg
      841 ccgcgccgcg tctttccccg gcgccgggtt ctgctagcgt cccggcgatg gcgtttgtcc
      901 cgctggttcc gatcggtgac ccgctgacca agctcgtcga cgaccgcacc gccggcgagc
      961 agcgttcggc gctcggcgcg ctcgaggcgc ggctgcgcga ccggcggcgc gaggtcgagg
     1021 ccggctgggg gccgacctac atcgatcgcg tccacaagaa gggcaagctg accgcgcgcg
     1081 agcggctggc ccggctcgcc gatccggata cgcgctgcta cgaggtcggg acgttcgtca
     1141 accacggcga ggtgttcccc ggcgatcagc gatcgcccgg cgccggcatc gtgaccgcgt
     1201 tcgcgcgggt cgccggacgg tggtgcatgg tgatcgccaa cgacaacacg gtggcgtcgg
     1261 gcgcgtggtg gccgcggacg cccgagaaga tccagcgcgc gcagaccatg gcgctgcggc
     1321 tgcggctgcc gacgatctac ctggtcgact gcagcggtct gttcctgccc gagcagagca
     1381 agagcttccc cggcgcgcgc ggcgcgggcc acatcttcaa gatgaacagc ctcttgtcgg
     1441 cgcacggcgt gccgcagatc gccggggtgt tcggcgactg catcgcgggc ggcggctaca
     1501 tgccgatcat ctccgaccgg gtctacatga ccgagcaggc gtacatggtg atcgccggcg
     1561 ccgcgctgat caagggcgcc aagagtcaga agatcacgtc gctcgacatc ggcgggcccg
     1621 agatccacgt ccacgcgtcg ggctgcgccg atgtccgggt tccggacgac gagaccctgc
     1681 tcgcctgcat ccggcgcgac gtcgcgcggc tgccgtcgag cggcgccgac ttctaccgcg
     1741 gcgagctcgc ggcgctcgat ccgcggttcg ccgccggcga gctcgccggc ctgttgccgg
     1801 tcgaccaccg cgaggcctac gacgcgaccc aggtgctcgc ccggctggtc gaccagtcgc
     1861 tgttctggga ggtgatgccc gaggtcggcg aggagatgat ctgcggcgtc ggccgggtcg
     1921 ccgggctgta cgccgggttc gtgatcaacc gccagggcct ggtcggcgat cccgacgatc
     1981 cgtcgcgcca gcgcccgggc ggcatcctgt accgcgccgg gatcgccaag atcagcgcgt
     2041 tctcgcgcgc gtgcaacgac gacggcctgc cgctgatctg gctgcaggac atcagcgggt
     2101 tcgacatcgg ccaccaggcc gaggcccacg gcctgctcgc ctacggcagc tcgctgatct
     2161 acaccaactc gaccaacacc acgccgatgt tcaccgtgct gctgcgcaag gcgtccggcg
     2221 ccggctacta cgcgatggcc ggcctgccct acgacccggt ggtccagct
//
LOCUS       DASVOR010001056         4553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_186183,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001056 DASVOR010000000
VERSION     DASVOR010001056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186183"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VF516_20295"
     CDS             <1..816
                     /locus_tag="VF516_20295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8110087.1"
                     /translation="NLAQAKLNLSYATIYSPVDGVVLSRAVDVGQTVAASLQAPVLFT
                     IAEDLARMQIDTAVAEGDVGRLTEGMKATFTVDAFPGRQFQGVVRQVRNAPTTTQGVV
                     TYDAVIDVDNTDHALRPGMTANVTFVLAQVADAIKIPNAALRFKPTREQLMAVFAQFG
                     GRNGRGSGGWGGSGGSGRRGSGDGPGGPGGPGGRGMMEGGMPGMPGGQRDLGDKKRVF
                     KLVDGHPRPVLIKPGLTDGSSTEMLDGDLKPGDELVTDILGVPTGPTRKLGAF"
     gene            816..1526
                     /locus_tag="VF516_20300"
     CDS             816..1526
                     /locus_tag="VF516_20300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8110088.1"
                     /translation="MAALIEMRGVERTYVMGDNLVHALRGVDLTIEAGSSIAIMGTSG
                     SGKSTMMNIMGCLDQPTRGSYLLNGKDVARLSRTQLADVRGRMIGFVFQSFNLLSRTS
                     ALENVEMPLMYQGIPGRERHRRAMRALEAVNLADRADHHSSQLSGGQQQRVAIARALV
                     GEAPVIMADEPTGNLDSKTSYEVMAILQGLVSQGKTVILVTHEADIAAYASRVVTLRD
                     GLIIDDKTQTPVKAEVAA"
     gene            1523..2752
                     /locus_tag="VF516_20305"
     CDS             1523..2752
                     /locus_tag="VF516_20305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8110089.1"
                     /translation="MNVFTTLKLAMRALWRNKVRTLLTMAGIVFGIGAVIAMVSGGQG
                     AQQAVKDVFQSLGTNVLILTNGSQRSFGAAGGAGSRQSLTWDDLKALQSGEIATIKWV
                     APMLQTRAQIASEDANWNTQVVGTTAIWFQIRNWPAVQGVAFDEDTGNSNAKVAVIGH
                     TVATQLFGTSNPVGQTIRISGQPYEVIGVLATKGQGPMGDYDDTLVIPVKTYEQKLSK
                     GLAKYISGQILISMVTDDVAERTMSDITALLRDRHKLDASDEDDFRIRNPAEFAQAQQ
                     DSTQRIATLLAIVAAVSLFVGGIGVMNIMLVSVIERTREIGIRMAVGAKPVDVMTQFL
                     VESLVLAALGGVLGLGLGAGAAKYMAGYFGWKFFFPAATAAIAFLVAGGVGVVFGLYP
                     AIRASRLDPITALRYET"
     gene            2749..4197
                     /locus_tag="VF516_20310"
     CDS             2749..4197
                     /locus_tag="VF516_20310"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8110090.1"
                     /translation="MKSVSLAFLAALSGVAFAQPGVTQPAAPAPSPGPSPGGPPPNQP
                     APSPVLPQPVDPTAPQTNSTGGEVLTLERAVEIAMRQQPSLRQSQAALEAARGRVDLA
                     RVARHPTIVLDATAGASSGLLRNQTTLPNGTVVTTSSGPDFFSHQESTGLGATANWRL
                     YDFGQTTANIRAAELNADAFAATIGTTTLDIRTGVEVAYLTAVADRKIVVVAETTVKS
                     EDNHLDQARRFVAAQAHDPIEVAQAQARAANARSALAQAQSNEAIALANLRSAIGWVD
                     PTRSPTVAPNWPIPPETEPQPLIALVDAARKNRPEILQLDKLLEAAGASLDAAHAERR
                     PVLSATAQTQWSPGTGNWSPQPSWSAGLTLAWPLFDGGRASADIRIANANVMTASANR
                     DALLVTLTSQLDAARSEIVANRANVAASTEAVSAARAQLQLSEARYTQGLGSQIELAD
                     AQTAVTTAEGNLVQAEFQLASAWAQLHRAVAQ"
     gene            4373..>4553
                     /locus_tag="VF516_20315"
     CDS             4373..>4553
                     /locus_tag="VF516_20315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110091.1"
                     /translation="MRQPLSAAEARKLAREILVQGAVSFTKHCLEELSKDGKTTVDAT
                     NVIRGGAYAEAEWENG"
BASE COUNT          728 a         1636 c         1571 g          618 t
ORIGIN      
        1 aacctggcgc aggccaagct caacctgtcg tacgcgacga tctactcgcc ggtcgacggc
       61 gtcgtgctgt cgcgcgcggt cgacgtcggc cagaccgtgg cggcgtcgct gcaggccccg
      121 gtcctgttca cgatcgccga ggacctcgcg cgcatgcaga tcgataccgc ggtcgccgag
      181 ggcgacgtcg gccggctgac cgagggcatg aaggcgacgt tcacggtcga cgccttcccc
      241 ggccggcagt tccagggcgt ggtccgccag gtccgcaacg cgccgaccac gacccagggc
      301 gtggtgacct acgacgcggt gatcgacgtc gacaacaccg atcacgcgct gcgccccggc
      361 atgaccgcca acgtcacgtt cgtgctcgcc caggtcgccg acgcgatcaa gatccccaac
      421 gccgcgctgc ggttcaagcc gacccgcgag cagctgatgg cggtgttcgc ccagttcggc
      481 gggcgcaacg ggcgcggcag cggcgggtgg ggcggctcgg gtggctcggg acggcgcggc
      541 agcggcgatg gcccgggcgg accgggcgga cccggcggcc ggggcatgat ggagggcggc
      601 atgccgggca tgcccggcgg gcagcgcgac ctcggcgaca agaagcgggt gttcaagctg
      661 gtcgacggcc atccccggcc ggtgctgatc aagcccggcc tgaccgacgg ctcgtcgacc
      721 gagatgctcg acggcgatct caagcccggc gacgagctcg tcaccgacat cctcggcgtg
      781 ccgaccggcc cgacccgcaa gctcggagcg ttctgatggc ggcgctgatc gagatgcgcg
      841 gggtcgagcg gacctacgtg atgggcgaca acctcgtcca cgcgctgcgc ggcgtcgacc
      901 tgacgatcga ggccggctcg tcgatcgcga tcatgggcac gtcgggctcc ggcaagtcga
      961 cgatgatgaa catcatgggc tgcctcgacc agccgacccg cggcagctac ctgctcaacg
     1021 gcaaggacgt ggcgcggctg tcgcgcaccc agctcgccga cgtgcgcggc cggatgatcg
     1081 gcttcgtgtt ccagagcttc aacctgctgt cgcggacctc tgcgctcgag aacgtcgaga
     1141 tgccgctgat gtaccagggc atcccgggcc gcgagcgcca ccgccgcgcg atgcgcgcgc
     1201 tcgaggcggt caacctcgcc gatcgcgccg accaccactc gagccagctg tcgggcggcc
     1261 agcagcagcg ggtcgcgatc gcgcgcgcgc tggtcggcga ggcgcccgtg atcatggccg
     1321 acgagccgac cggcaacctc gacagcaaga cgtcgtacga ggtgatggcg atcctgcagg
     1381 gcctggtcag ccagggcaag accgtgatcc tggtgaccca cgaggccgac atcgcggcct
     1441 acgcgtcgcg cgtggtcacc ctgcgcgacg ggctgatcat cgacgacaag acccagacac
     1501 cggtcaaggc ggaggtcgcg gcatgaacgt cttcaccacc ctcaagctcg cgatgcgcgc
     1561 gctgtggcgc aacaaggtgc gcacgctgct caccatggcg ggcatcgtgt tcggcatcgg
     1621 cgcggtgatc gcgatggtgt cgggcggtca gggcgcgcag caggcggtca aggacgtgtt
     1681 ccagagcctg ggcaccaacg tcctgatcct gaccaacggc tcgcagcgca gcttcggcgc
     1741 cgccggcggc gcgggctcgc gccagtcgct gacctgggac gatctcaagg cgctgcagag
     1801 cggcgagatc gccacgatca agtgggtcgc gccgatgctg cagacccggg cccagatcgc
     1861 gtcggaggac gccaactgga acacccaggt ggtcggcacc accgcgatct ggttccagat
     1921 ccgcaactgg ccggcggtcc agggcgtggc gttcgacgag gacaccggca acagcaacgc
     1981 caaggtcgcg gtgatcggcc acaccgtcgc gacccagctg ttcggcacgt cgaacccggt
     2041 cggccagacc atccggatct cgggccagcc ctacgaggtg atcggcgtgc tcgccaccaa
     2101 gggtcagggc ccgatgggcg actacgacga caccctggtg atcccggtca agacctacga
     2161 gcagaagctg tccaagggcc tggccaagta catcagcggc cagatcctga tctcgatggt
     2221 caccgacgac gtcgccgagc gcacgatgtc cgacatcacc gccctgctgc gcgatcgcca
     2281 caagctggac gcctccgacg aggacgactt ccggatccgg aacccggcgg agttcgccca
     2341 ggcccagcag gactcgaccc agcggatcgc gacgctgctc gcgatcgtcg ccgcggtgtc
     2401 gctgttcgtc ggcgggatcg gcgtgatgaa catcatgctg gtcagcgtca tcgaacggac
     2461 ccgtgagatc ggcatccgca tggcggtcgg cgccaagccg gtcgacgtca tgacccagtt
     2521 cctcgtcgag tcgctcgtgc tcgccgcgct cggcggcgtg ctgggcctcg ggctcggcgc
     2581 gggggccgcc aagtacatgg cgggctactt cggctggaaa ttcttcttcc cggcggccac
     2641 cgcggcgatc gcgttcctgg tcgccggcgg cgtcggggtg gtgttcggtc tctatccggc
     2701 gatccgcgcc tcgcggctcg atccgatcac ggctctgagg tacgagacat gaagtcggtt
     2761 tctctggcat tcctggccgc gctgagcggc gtcgccttcg cgcagcccgg ggtcacccag
     2821 ccggcggcgc cggccccatc tccgggtccc tcccccggcg gccctccgcc gaaccagccc
     2881 gcgccgagcc cggtgctgcc ccagccggtc gatccgacgg ccccgcagac caacagcacc
     2941 ggcggcgagg tgctgacgct cgagcgcgcg gtcgagatcg cgatgcggca gcagccgtcg
     3001 ctgcgccaga gccaggccgc gctcgaggcc gcgcgcggtc gggtcgatct cgcgagggtg
     3061 gcgcgccacc cgacgatcgt gctcgacgcg accgcgggtg ctagctcggg gctgctgcgc
     3121 aaccagacca cgcttcccaa cggcacggtc gtcacgactt ccagtggccc ggacttcttc
     3181 agccaccagg agtcgaccgg gctcggcgcg acggcgaact ggcggctcta cgatttcggc
     3241 cagacgaccg ccaacatccg cgccgcggag ctcaacgccg acgcgttcgc ggccacgatc
     3301 ggcacgacga cgctcgacat ccgcacgggc gtcgaggtcg cgtacctcac cgcggtcgcc
     3361 gatcgcaaga tcgtggtggt cgccgagacc accgtcaaga gcgaggacaa ccacctcgat
     3421 caggcgcggc ggttcgtcgc cgcccaggcc cacgatccga tcgaggtcgc gcaggcccag
     3481 gcgcgcgccg ccaacgcgcg gtcggcgctg gcccaggccc agagcaacga ggcgatcgcg
     3541 ctggccaacc tgcgctcggc gatcggctgg gtcgatccga cccggtcgcc gaccgtggcg
     3601 ccgaactggc cgatcccgcc cgagaccgag ccgcagccgc tgatcgcgct ggtcgacgcc
     3661 gcgcgcaaga accggcccga gatcctccag ctcgacaagc tgctcgaggc cgccggcgcc
     3721 agcctcgacg ccgcgcacgc cgagcgccgg ccggtgctgt cggcgaccgc gcagacccag
     3781 tggtcgccgg gcaccggcaa ctggtcgccg cagccgagct ggagcgccgg gctgaccctg
     3841 gcgtggccgc tgttcgacgg cggcagggcg tcggccgata tccggatcgc caacgccaac
     3901 gtcatgaccg cctcggccaa ccgcgacgcg ctgctggtca ccctgacctc gcagctcgac
     3961 gccgcgcgct ccgagatcgt cgccaaccgc gccaacgtcg ccgcgtcgac cgaggcggtg
     4021 tccgccgcgc gcgcccagct ccagctgtcc gaggcgcgct acacccaggg cctcggcagc
     4081 cagatcgagc tcgccgacgc ccagaccgcg gtgaccaccg ccgagggcaa cctggttcag
     4141 gccgagttcc agctcgcgtc cgcgtgggcc cagctccacc gcgcggtcgc ccagtaatag
     4201 ccgcagcctt cctcacgcac cgcgacgttt cgcgagcaca tggcgggtcg gctccaggaa
     4261 gcacctggta gcctcgggtg atccgcaggc attgcggtag cttggcaggc tcacccgagc
     4321 tatcctaaga tcaatcattg acaaaagatc atgccgccct aagatggagg acatgaggca
     4381 gccgctctct gctgccgagg ctcgcaagct ggctcgcgag atcctcgtcc agggggcggt
     4441 cagcttcacg aagcactgcc tggaggagct cagcaaggat gggaagacga ccgtcgatgc
     4501 aacgaacgtc attcgtggcg gcgcttacgc cgaggctgaa tgggaaaatg gcg
//
LOCUS       DASVOR010001057         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_98817,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001057 DASVOR010000000
VERSION     DASVOR010001057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98817"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VF516_20320"
     CDS             <1..502
                     /locus_tag="VF516_20320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8110092.1"
                     /translation="AVVGKSFARSASAMDDADLASSLDANLWSAARIAQRAVPHLIAR
                     GGGSITMLSSIWGREAGGAPGYNTAKAAVIALAKALARDYARHAIRVNSVAPGSILFP
                     GGGWDRRRNADPEGIAAFVEREIPMGRFGTPDEVAEVVAFLASARARWITGACIPVDG
                     GQGRSF"
     gene            933..>1501
                     /locus_tag="VF516_20325"
     CDS             933..>1501
                     /locus_tag="VF516_20325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110093.1"
                     /translation="MTSSSEAPVPTVRIEVTLGETVGRYQVRRQLGSGGMGQVYLARD
                     VVLGRSVALKIVGGNRSIGVPTEQFLHEARAIARLNHPNIVQLYDFGEYKGGFYLALE
                     YVEGDTLRERVRDGTMALDEALRHARAIADGLCHAHDNGVYHCDLKPSNVMVGKDGRV
                     RVVDFGIARTGDSATSASSGTPDWMAPEQ"
BASE COUNT          224 a          509 c          553 g          215 t
ORIGIN      
        1 cgccgtggtc ggcaagtcgt tcgcccgcag cgcctcggcg atggacgacg ccgacctcgc
       61 gagctcgctc gacgccaacc tgtggagcgc ggcgcggatc gcgcagcgcg cggtgccgca
      121 cctgatcgcg cgcggcggcg gctcgatcac gatgctgtca tcgatctggg gccgcgaggc
      181 cggcggcgcg cccggctaca acaccgccaa ggcggcggtg atcgcgctcg ccaaggcgct
      241 ggcccgcgac tacgcgcgcc acgcgatccg cgtcaactcg gtcgcgcccg gctcgatcct
      301 gttcccgggc ggcggctggg accggcggcg gaatgccgac cccgagggca tcgcggcgtt
      361 cgtcgagcgc gagatcccga tgggccggtt cggcacgccc gacgaggtcg ccgaggtggt
      421 cgcgttcctc gcgtcggcgc gggcgcgctg gatcaccggc gcctgcatcc cggtcgacgg
      481 cggccagggc cgcagcttct aacgctcgtt cggtggttcg atcctccgag ctgcctcacg
      541 gggtggagga tgtccaccac cggcggacag gtatgcccga ggtgcgcagc cgggcggcct
      601 gacaggacac tgcttgcttt ccgataccgc tgttccggaa agatctcgga tagcgcgctg
      661 tgggagagcg ctgtggcaca cgcagccgat cgcgccgacc ggtccgatgc caggtcgtcg
      721 agcgggcgcg agcgcagcta caccagcata ccgggaatga ccgggtcaga gacctgacgc
      781 accggacgca gaggtcagat gttttgtctg ccttacgttg cttacagctg accggccagc
      841 gccgcgatgc ggcttgcgag atcgcggaac agtccgtagc ttctgcgggg atcgaggaca
      901 tggtggcgga acagccgacg ctctcgcttg tggtgacctc ctcctcggag gcaccggtac
      961 cgacggtccg catcgaggtc acgctcggcg agaccgtcgg gcggtaccag gtgcggcgcc
     1021 agctcggcag cggcggcatg ggccaggtct acctcgcgcg cgatgtcgtg ctcggccggt
     1081 cggtcgcgct gaagatcgtg ggtggaaacc gcagcatcgg ggtgccgacc gagcagttcc
     1141 tccacgaggc gcgggcgatc gcccggctca accacccgaa catcgtgcag ctgtacgact
     1201 tcggcgagta caagggcggg ttctacctcg cgctggagta cgtcgagggc gacacgctgc
     1261 gcgagcgggt gcgcgacggc acgatggcgc tcgacgaggc gctgcgccac gcccgcgcga
     1321 tcgccgatgg cctgtgccac gcccacgaca acggcgtgta ccactgcgac ctcaagccga
     1381 gcaacgtgat ggtcggcaag gacgggcggg tccgcgtggt cgacttcggg atcgcgcgca
     1441 ccggcgacag cgcgacgagc gcgtcgtccg gaacgccgga ctggatggcg ccggagcagt
     1501 g
//
LOCUS       DASVOR010001058         1750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_361406,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001058 DASVOR010000000
VERSION     DASVOR010001058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361406"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..598)
                     /locus_tag="VF516_20330"
     CDS             complement(119..598)
                     /locus_tag="VF516_20330"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEX8110094.1"
                     /translation="MPKQPVTMAIRALRAAGVAFVPHLYPYEPRGGTRASSAALGVPE
                     HSVVKTLIFETERGDPLCILMHGDREVSVKQLARVIGTKTVAPCAPEVADRHSGYQVG
                     GTSPFGLRRAMPSYMQSTILELPRVYINGGARGFLVELDPRDVQRVLAPVLVDVAVD"
     gene            628..1644
                     /gene="dmeF"
                     /locus_tag="VF516_20335"
     CDS             628..1644
                     /gene="dmeF"
                     /locus_tag="VF516_20335"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HEX8110095.1"
                     /translation="MQAHDLSEHVHHEDEHAYNRVGERRTRWVILITAVMMLGELVVG
                     WWTGSVALTADGWHMGTHVGALGLTLAAYWYARTRAGHDTFSFGTGKVYALAGYTSGV
                     LLALVALWMAYEGIHNLVTHPVVDYRDSLPVAALGFVVNIVSTALLSRGHQYGHDHAG
                     HGHGAHGHGRAAARPAPKPGTLDFNLRAAYIHIFADAMTSLLAVAALALGWWAGLWYL
                     DPLMGLIGGGVIVWWAVSLCRQAARQLLDVVSSPHHERIVRERLEAIDDVRVADLHVW
                     ELGPGRRSCIVSVVTARPREVSDYRDAVLSALDVSHLTIEIHRCGLAHGAPRPVAEPA
                     HHDH"
BASE COUNT          246 a          621 c          625 g          258 t
ORIGIN      
        1 ccgggtgggc ggtgggaagg cacggagggc gagggcgcca cgcacgaggc cagcagtcac
       61 aatgaccagg aggttgattc aacggctgtc cggatgcaac tggaaggtgc cccaccggtc
      121 agtccaccgc gacgtcgacc aggacggggg ccagcacgcg ctggacatcg cgcggatcga
      181 gctcgaccag gaagccgcgc gcgccgccgt tgatgtagac ccgcggcagc tcgaggatcg
      241 tcgactgcat gtagctcggc attgcgcgtc gcaagccgaa gggtgaggtt cctcccacct
      301 gatagcccga atgccggtcg gcgacctccg gggcacaggg cgccacggtc ttggtcccga
      361 tcacccgcgc gagctgcttc accgagactt cacggtcgcc atgcatgagg atgcacagcg
      421 gatcgccgcg ctcggtttcg aagatcaatg tcttgacaac gctgtgctcc gggaccccga
      481 gcgccgcgct cgacgcccgg gtcccgccgc gcggctcgta cggatagaga tgcgggacga
      541 acgccacccc ggcggcccgc agcgcgcgga tcgccatcgt caccggctgc ttgggcacgc
      601 gagatgatgg gatagactgc cgcctccatg caagcgcacg acctgtccga gcacgtccat
      661 cacgaggacg agcacgcgta caaccgggtc ggcgagcggc gcacacggtg ggtcatcctg
      721 atcacggccg tgatgatgct cggcgagctg gtcgtcgggt ggtggaccgg ctcggtcgcg
      781 ctcacggcgg atggctggca catggggacc cacgtcggcg cgctcggcct gacgctggcg
      841 gcctactggt acgcgcgcac ccgggccggg cacgacacgt tcagcttcgg caccggcaag
      901 gtctatgcgc tcgccggcta caccagcggc gtgctgctcg cgctggtcgc gctgtggatg
      961 gcctacgagg gcatccacaa cctggtcacc caccccgtgg tcgactaccg cgactcgctg
     1021 ccggtcgcgg cgctgggctt cgtggtcaac atcgtgtcga ccgcgctgct cagccgcggc
     1081 caccagtacg gccatgacca cgccggccat ggccacggcg ctcacggcca tggccgggcc
     1141 gccgcccgcc ccgcgccgaa gccgggcacg ctcgatttca acctgcgcgc cgcgtacatc
     1201 cacatcttcg ccgacgcgat gacgtcgctg ctggcggtcg ccgcgctggc gctcggctgg
     1261 tgggcgggcc tgtggtacct cgacccgctg atgggcctga tcggcggcgg cgtgatcgtg
     1321 tggtgggcgg tcagcctgtg ccgccaggcc gcgcgccagc tgctcgacgt cgtgtcgtcg
     1381 ccgcaccacg agcggatcgt gcgcgagcgg ctcgaagcga tcgacgacgt ccgggtcgcc
     1441 gatctccacg tctgggagct cgggccgggc cggcgtagct gcatcgtctc ggtggtcacc
     1501 gcgcggccgc gcgaggtgtc cgactaccgc gacgccgtgc tgtcggcgct cgacgtgtcc
     1561 cacctgacga tcgagatcca ccgctgcggg ctcgcgcacg gcgcgccgcg gcccgtcgcc
     1621 gagccggcgc accacgacca ctgagaccac cgggcgccgg cgctactcgt cgctggggcg
     1681 cgtcgagttg cgcttgcggc gcggcttgtt gctcgcggtc gcgatcgccg cggtgttgac
     1741 cgtcggcgcg
//
LOCUS       DASVOR010001059         1692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_40030,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001059 DASVOR010000000
VERSION     DASVOR010001059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40030"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VF516_20340"
     CDS             complement(<1..717)
                     /locus_tag="VF516_20340"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8110096.1"
                     /translation="MKIRFWGVRGSIPAPGPDTIRYGGNTACVSVHARAGGLVILDMG
                     TGLMHLGNALMAGPFGDGRGSATILLSHTHWDHIQGLGFFAPVFVAGNAFTVYGPGGS
                     SDVLEDILEGQMDPNFSPLQTLKNFSARFDVHAIELGAPLSAGGLTITAREHPHGATT
                     ALAFRITEPAAPTGAPERTFVYASDVGQPTAAGPDPAAVAFYRGADVLLHDATYRPAD
                     QATRRNRGSRPTRTPRTPPSP"
     gene            complement(714..>1692)
                     /locus_tag="VF516_20345"
     CDS             complement(714..>1692)
                     /locus_tag="VF516_20345"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8110097.1"
                     /translation="RPPAGLRSGWRPAPTMKVRFWGVRGSIPVPGRTTSRYGGNTSCV
                     EVRPQGAAPIIIDAGTGLRRLGKALMEDAFGDGRGEASILISHTHWDHVQGLPFFSPL
                     YRAGNRIQIFARQRDTHLAAVFSQQHGAPYFPVPLSAMQAEMSFHALTEGARFELGRA
                     RITSTRLNHPWTALAYRIDVDRAAVVYCSDTAPFTDLLLGREFMVDAPALDRPLPPDV
                     AAELARMRAAVVGLAAGADLLIYDTQFTHDEYRLRPHWGHSHPDDALAIARDARVRRL
                     CLFHHAPLRSDDDNDAILARYRGVAAAAGDPFEVLSAYEGLELTLGDGP"
BASE COUNT          229 a          528 c          719 g          216 t
ORIGIN      
        1 aggcgacggc ggcgtccgcg gcgtcctcgt aggacgagag ccgcggttgc ggcgggtcgc
       61 ctggtcggcg ggccggtagg tcgcgtcgtg gagcagcacg tcggcgccgc ggtagaacgc
      121 gacggcggcg gggtcggggc cggcggcggt gggctggccg acgtcggagg cgtagacgaa
      181 ggtgcgctcg ggcgcgccgg tgggggcggc gggttcggtg atccggaacg cgagcgccgt
      241 ggtcgcgccg tgcgggtgct cgcgggcggt gatggtgagg cccccggcgg acagcggggc
      301 gccgagctcg atcgcgtgga cgtcgaaccg ggccgagaag ttcttgaggg tctggagcgg
      361 cgagaagttg ggatccatct ggccctcgag gatgtcctcg aggacgtcgg acgagccgcc
      421 cgggccgtac acggtgaacg cgttgccggc gacgaacacg ggggcgaaga agcccaggcc
      481 ctggatgtga tcccagtggg tgtgggacag caggatcgtc gccgagccgc ggccgtcgcc
      541 gaacgggccg gccatcagcg cgttgccgag gtgcatgagg ccggtgccca tgtcgaggat
      601 gaccaggccg ccggcgcggg cgtggaccga gacgcacgcg gtgttgccgc cgtagcggat
      661 cgtgtcgggg ccgggcgccg ggatcgagcc gcggacgccc cagaaccgga tcttcatggg
      721 ccgtccccca gggtcagctc gaggccctcg taggcgctca gcacctcgaa cggatcgccg
      781 gcggcggcgg cgacgccgcg gtagcgggcg aggatcgcgt cgttgtcgtc gtcgctgcgc
      841 agcggcgcgt ggtggaacag gcacagccgg cggacgcgtg cgtcgcgggc gatggcgagc
      901 gcgtcgtcgg ggtgggagtg gccccagtgc ggccgcagcc ggtactcgtc gtgggtgaac
      961 tgggtgtcgt agatcaggag gtcggcgccg gcggcgagcc cgacgaccgc ggcgcgcatc
     1021 cgggcgagct cggcggcgac gtcgggcggc agcgggcgat cgagcgccgg ggcgtcgacc
     1081 atgaactcgc ggccgagcag gaggtcggtg aacggcgcgg tgtccgagca gtagacgacc
     1141 gcggcgcggt cgacgtcgat ccggtaggcc agcgcggtcc acgggtggtt gagccgggtc
     1201 gaggtgatcc gggcgcggcc gagctcgaac cgggcgccct cggtgagggc gtggaacgac
     1261 atctcggcct gcatcgcgga cagcgggacc gggaagtacg gcgcgccgtg ctgctgggag
     1321 aacaccgccg cgaggtgggt gtcgcgctgg cgcgcgaaga tctggatccg gttgccggcg
     1381 cggtagagcg gggaaaagaa cggcaagccc tggacgtggt cccagtgggt gtggctgatg
     1441 aggatcgagg cctcgccgcg gccgtcgccg aacgcgtcct ccatcagcgc cttgccgagc
     1501 cggcgcaggc cggtgccggc gtcgatgatg atcggcgcgg cgccctgcgg ccggacctcg
     1561 acacacgacg tgttgcctcc gtaccggctg gtcgtgcgcc cgggtacggg gatcgaacca
     1621 cggactcccc agaacctgac cttcatcgtg ggcgccgggc gccatccgga ccgtaacccc
     1681 gccgggggcc tg
//
LOCUS       DASVOR010001060         2450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_127366,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001060 DASVOR010000000
VERSION     DASVOR010001060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127366"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VF516_20350"
     CDS             complement(<1..1282)
                     /locus_tag="VF516_20350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110098.1"
                     /translation="MRRHYSGHVRRASTNRQPFREVDRGNELHVFDVARNTKLSPSPD
                     EREQRIVSVALQSAMIYRAARGDERDMVEVGALLAGKYRIDRVLGQGGMGLVVAATHV
                     HLAQRVALKFLLPEFLHDPAIVERFLREARASAALRGEYLCRVSDVGTLDNGSPYIVM
                     ELLDGRDLAKVLAENGPLPIETASHHVLQACVALAEAHALGIVHRDLKPANLFLAGRP
                     DGTQLVKVLDFGIAKAQHDTSFNLTRTASVMGSPGYMSPEQLRSSRDADVRSDIWALG
                     VILYELVSGRPPYVAESITELALRVAMDPLPPLAIAAPTGFDRVVMRCLEKDPARRFQ
                     NVAALAQALVPFGGHRARELAAGAARILNVVAASTSSHDIAVSNAPTTLSSSAGSATR
                     VRQRFPWAVLVGAVAVTIAAIIAVFELRSGGDEAT"
     gene            1281..>2450
                     /locus_tag="VF516_20355"
     CDS             1281..>2450
                     /locus_tag="VF516_20355"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX8110099.1"
                     /translation="MQMSAGRRAVLCASSTLVLGCNQLLGVGELHESKQGDAGLAIDA
                     ASDAPPGGQAVLVLDTGTYQFGTVVTGASPVEHTFTISNTGTAASGPVHAEITGTAMS
                     SFSIGTNGCTGALDAGRSCAIAVRFAPVATGPVDASLHVTASPGGDLGTALSGTGLAP
                     GALIVTPDSVSYGLIVVNGVSSPQTLSVKNTGGADSGAIDVQLTGSDAGQFALEPPAI
                     GDCQGATLAMNQICKVRLHFAPTGPGSKSASVVVSSASAGTISGSLVGTGVNALTATP
                     AVHDFNVLGTGDSSAPFAFTIGNGSATTTGPIMFALGGADAGQFTIVSPGPGDCILGT
                     TALAMGASCTVRVRFAPTMVGAKSASLAFTASPGGNASATATGTAVTPAALAVAGT"
BASE COUNT          406 a          848 c          826 g          370 t
ORIGIN      
        1 ccgtggcttc gtcgccaccg ctgcgtagct cgaagaccgc gatgatcgcc gcgatcgtaa
       61 ccgcaacggc tccgacgagg acggcccacg ggaaccgctg gcggacgcgc gtcgccgaac
      121 cggccgacga gctgagcgtg gtgggcgcat tcgacaccgc gatgtcgtgc gatgacgtcg
      181 atgctgcgac cacgttgagg atccgtgccg cgccggccgc gagctcgcgt gctcggtggc
      241 cgccgaacgg gacgagagcc tgcgcgaggg cagcaacatt ttggaatcgc cgcgcggggt
      301 ctttctcgag acaccgcatc acgacgcgat cgaaaccagt cggcgccgca atggccagcg
      361 gcggtaacgg atccatcgcg acgcgaagcg caagctcggt gatgctctcg gcgacatacg
      421 gaggccgacc cgacaccagc tcgtagagga tcacgccgag tgcccagatg tcactccgga
      481 cgtcggcatc acggctcgac cgcagctgtt cgggcgacat gtagcccggg gatcccatca
      541 cgctcgccgt cctggtcagg ttgaacgacg tgtcgtgctg cgccttggcg atgccgaagt
      601 cgaggacctt gacgagctgt gtcccatcgg gacgcccggc gaggaacagg ttcgccggct
      661 tcagatcgcg atgtacgatc ccgagcgcat gcgcctcggc gagggccacg catgcctgca
      721 ggacatggtg actggccgtc tcgatcggca gcggtccgtt ctcggcgagg accttcgcga
      781 ggtcacgacc atcgagtagc tccatcacga tgtacggtga cccgttgtcg agcgtcccga
      841 catcggagac ccgacacagg tactcgccgc gcaacgccgc tgatgcacgc gcttcgcgca
      901 ggaaacgctc gacgatggcc ggatcgtgga ggaactcggg cagcaggaac ttgagcgcta
      961 cccgttgcgc gagatgaacg tgtgtagcgg cgacgacgag tcccatcccg ccctgaccca
     1021 gcacccgatc gatccggtac ttccctgcga gcagcgcgcc gacttcaacc atgtctctct
     1081 cgtcaccccg ggcagcacga tagatcatcg ctgactggag cgccacggaa acaatgcgtt
     1141 gttcgcgctc gtcaggcgat ggtgaaagct tggtattgcg ggcaacatca aagacatgaa
     1201 gctcgtttcc cctgtcgacc tcacgaaatg gttgtctgtt tgtacttgcg cggcggacat
     1261 ggcctgaata gtgtcgccgc atgcagatga gtgcggggag gcgggcggtg ctctgcgcgt
     1321 cgtcgaccct ggtactgggg tgcaatcagc tcctcggcgt gggcgagctc cacgagagca
     1381 agcagggtga cgccgggctg gcgatcgatg cggcaagcga cgcgccgccc gggggtcagg
     1441 ccgtgctcgt gctcgacacc ggcacatacc agttcggaac cgtcgtgacc ggagcctcgc
     1501 ccgtcgaaca cacgttcacg atcagcaaca ccggcaccgc cgcctcgggt ccggtgcacg
     1561 ccgagatcac cggcacggca atgtcgtcgt tctcgatcgg caccaacggc tgcaccggcg
     1621 cgctggacgc cggcaggagc tgcgcgatcg cggtgcgctt tgccccagtc gccacggggc
     1681 ccgtcgacgc ctcgctgcac gtgaccgcgt cgccgggcgg cgacctcgga accgcgctat
     1741 cgggcaccgg cctggcgccg ggcgcgctga tcgtcacgcc ggactcggtg tcgtacgggc
     1801 tgatcgtggt caacggggtc agctcgccgc agacgctctc ggtcaagaac accggcggtg
     1861 ccgactcggg cgcgatcgac gtgcagctca ccggcagcga cgccggccag ttcgcgctcg
     1921 agccgccggc gatcggtgac tgccagggcg cgaccctcgc gatgaaccag atctgcaagg
     1981 tacgccttca cttcgcgccc accggccccg gcagcaagtc cgcgtcggtg gtcgtgtcga
     2041 gcgcgagcgc ggggacgatc tcgggctcgc tggtcgggac cggcgtcaac gcgctgacgg
     2101 cgacgccggc cgtgcacgac ttcaacgtgc tcggcaccgg cgactccagc gcacccttcg
     2161 cgttcacgat cggcaacggc agcgcgacca cgaccggacc gatcatgttc gcgctcggtg
     2221 gcgccgacgc cggtcagttc acgatcgtct cgcccggccc gggcgactgc atcctcggca
     2281 ccaccgcgct ggcgatgggc gccagctgca cggtgcgcgt gcgcttcgca ccgaccatgg
     2341 tcggcgccaa gagcgcatcg ctcgcgttca ccgccagccc gggtggcaac gccagcgcca
     2401 ccgccaccgg caccgcggtc acgccggcag cgctcgccgt ggccgggacc
//
LOCUS       DASVOR010001061         3235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_419116,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001061 DASVOR010000000
VERSION     DASVOR010001061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3235
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419116"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VF516_20360"
     CDS             complement(<1..692)
                     /locus_tag="VF516_20360"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8110100.1"
                     /translation="MTAIATRTALLMAALIALLAIGGWAFAGRSGLVVMAIVGLAINF
                     GSYWFSDRIALLASGAQPVSRDEAPVLYAIVERLAAQAGMAMPSIHLIPSPSPNAFAT
                     GRGPGHAAVAVTEGLLRVVDRRELTGVLAHEISHVRNRDVLVATVAAGIAGVISTIGY
                     ALQWGLMFGNLDRDARRGSPLAALAWVVVAPIIALLLQLAISRAREYGADTSGARLTG
                     DPDGLADALERI"
     gene            complement(757..1710)
                     /gene="egtD"
                     /locus_tag="VF516_20365"
     CDS             complement(757..1710)
                     /gene="egtD"
                     /locus_tag="VF516_20365"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:NF021530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030745 - dimethylhistidine
                     N-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEX8110101.1"
                     /translation="MKPTIQAIRRHDPFAGDDVIAGLRAPHKHLPCKLLYDARGAALF
                     EQICTLDEYYPYRSELALLERHLPEIARVIGPAARVIEPGSGAGKKTRMLLAALDRPA
                     SYVPIDVSAEQLADTARELAAALPGLEVVPIVADYTRPLELPPPAPGTGRTCVFFPGS
                     TIGNFEPDDAHRFLARLAELAGPGGAIVLGADSTSDPDALVRAYDDPHGVTAAFDLNV
                     LAHLNRSHAANFDLSAFLHRAVWNAARSRIEMHLVSRRRQVVEVAGEAIALDRGEAIV
                     TEHCYKHPPEALAALLTAAGWRVGQVFADPDGRMRLWLAER"
     gene            complement(1767..3056)
                     /gene="egtB"
                     /locus_tag="VF516_20370"
     CDS             complement(1767..3056)
                     /gene="egtB"
                     /locus_tag="VF516_20370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEX8110102.1"
                     /translation="MVPSRTSRAHRSAPTDSARAESLRERFLAVRARTIALCAPLSAE
                     DQVVQPMPDASPAKWHLAHTTWFFEAFVLGGEPFDPAFEFLFNSYYESVGPRVPRPRR
                     GMLTRPGLDRIHAYRADVDHRVVAALQGGALGDDACARLELGLHHEQQHQELILTDAK
                     YVLGTQTLVPAYRALARGEPGPPTPLTWRSETGGIVEIGAPASGFAFDNERPRHRVLV
                     PPYRIASRPVANAEVLAFIADGGYRDPRLWLSDGWQLVRAERWTAPLYWSERDGEHAI
                     YDLAGVRAVDPGETACHLSLYEADAIARWLGARLPTEAEWEHAATQGTLHLRSNFADA
                     ERLHPDRAPAAGPGQLTQLFGDVWEWTASSYAAYPGFRPLPGALAEYNGKFMSGQYVL
                     RGGSCFTPADHIRPSYRNFFPAHARWQMTGVRLASDP"
     gene            3055..>3235
                     /locus_tag="VF516_20375"
     CDS             3055..>3235
                     /locus_tag="VF516_20375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110103.1"
                     /translation="MAAQPWPDPPREATTIVRIVTGGCQVCDRERRNLRNAGEQDEEY
                     RSGLISRDPLQRSVKD"
BASE COUNT          455 a         1103 c         1249 g          428 t
ORIGIN      
        1 tcgatccgct cgagcgcgtc ggcgagcccg tcgggatcgc cggtcagccg cgcgcccgag
       61 gtgtcggcgc cgtactcgcg ggcgcggctg atcgcgagct ggagcagcag cgcgatgatc
      121 ggcgcgacga ccacccaggc gagcgcggcg agcggtgagc cgcggcgagc gtcgcggtcg
      181 aggttgccga acatcaggcc ccactgcagg gcgtagccga tcgtcgagat cacgccggcg
      241 atcccggcgg cgaccgtcgc gaccagcacg tcgcggttgc ggacgtgcga gatctcgtgg
      301 gcgagcacgc cggtgagctc gcggcgatcg acgacccgga gcaggccctc ggtcaccgcg
      361 accgccgcgt ggccgggccc gcgcccggtc gcgaacgcgt tcgggctcgg cgacgggatg
      421 aggtggatcg acggcatcgc catgccggcc tgcgcggcca ggcgctcgac gatcgcgtag
      481 agcacgggcg cctcgtcgcg cgacaccggc tgtgcgccgc tggcgagcag ggcgatccgg
      541 tcggagaacc agtacgatcc gaagttgatc gccaggccga cgatcgccat caccacgagg
      601 ccgctccgtc cggcgaacgc ccagccgccg atcgcgagca gggcgatcag cgcggccatg
      661 agcagggcgg tcctcgttgc gatcgcggtc atgcgatcag cagcagcaac tcacgtgccg
      721 cggcggcccg ggccgggcgg ggctcgactc gccggctcag cgttcggcga gccagagccg
      781 catccggccg tcggggtcgg cgaatacctg accgacgcgc cagccggcgg cggtgagcag
      841 cgcggcgagc gcctcgggcg ggtgcttgta gcagtgctcg gtcacgatcg cctcgccgcg
      901 gtcgagcgcg atcgcctcgc cggcgacctc gacgacctgg cggcgccggc tgaccaggtg
      961 catctcgatc cgcgagcgcg cggcgttcca caccgcgcgg tggaggaacg cggacaggtc
     1021 gaagttcgcg gcgtggctgc ggttgaggtg cgcgagcacg ttgagatcga acgcggcggt
     1081 cacgccgtgc ggatcgtcgt acgcgcgcac cagcgcgtcg gggtcgctgg tcgagtcggc
     1141 gccgagcacg atcgcgccgc cggggccggc gagctcggcg agccgcgcga ggaaccggtg
     1201 ggcatcgtcg ggctcgaagt tgccgatcgt cgagcccggg aagaacacgc aggtccgccc
     1261 ggtgccgggc gccggcggcg gcagctccag cggccgggtg tagtcggcga cgatcggcac
     1321 gacctcgagg ccggggagcg ccgcggcgag ctcgcgcgcg gtgtcggcga gctgctcggc
     1381 gctgacgtcg atcggcacgt agctcgccgg ccggtcgagc gcagcgagca gcatccgggt
     1441 cttcttgccc gcgccgctgc cgggctcgat cacgcgcgcg gcggggccga tcacccgggc
     1501 gatctccgga aggtggcgct cgagcagcgc gagctcggag cggtacgggt agtactcgtc
     1561 gagcgtgcag atctgctcga acagcgccgc gccgcgcgcg tcgtacagca gcttgcacgg
     1621 caggtgcttg tgcggtgcgc gcagccccgc gatcacgtcg tcgcccgcga acgggtcatg
     1681 gcggcgaatg gcctggatcg tgggtttcat cgtcctctcc cttacgtcga acgcgtgggt
     1741 tggtatcgcc ggggctacgg atcggttcac ggatcgcttg caagccgtac gccggtcatc
     1801 tgccagcgcg cgtgggccgg aaagaagttg cgataggacg ggcggatgtg atccgccgga
     1861 gtgaagcacg atccgccgcg cagcacgtac tggccgctca tgaacttgcc attgtactcc
     1921 gcaagcgcgc cgggcagcgg acgaaatccc ggatacgccg cgtagctgct cgcggtccac
     1981 tcccagacat cgccgaacag ctgggtgagc tggccggggc ccgccgccgg cgcgcgatcg
     2041 gggtggaggc gctcggcgtc ggcgaagttg cttcgcaggt ggagcgtgcc ttgcgtcgcg
     2101 gcgtgctccc actcggcctc ggtcggtagt cgcgcaccga gccagcgcgc gatcgcgtcg
     2161 gcctcgtaca ggctgaggtg gcacgcggtc tcgccggggt cgaccgcgcg cacgccggcg
     2221 agatcgtaga tcgcgtgctc gccgtcgcgc tcgctccagt acagcggtgc ggtccaccgc
     2281 tcggcccgca cgagctgcca gccgtcgctc agccacagcc ggggatcgcg gtagccgccg
     2341 tcggcgatga acgccaggac ctcggcgttg gcgaccggcc gcgacgcgat ccggtagggc
     2401 ggcaccagca cgcggtggcg cggccgctcg ttgtcgaacg cgaagccgct ggcgggcgcg
     2461 ccgatctcga cgatgccgcc ggtctcgctg cgccaggtca gcggggtggg cggcccgggc
     2521 tcgccgcgcg ccagggcgcg gtacgcgggg acgagcgtct gggtgccgag cacgtacttg
     2581 gcgtcggtga ggatcagctc ctggtgctgc tgctcgtggt gcaggccgag ctcgagccgc
     2641 gcgcacgcat cgtcaccgag cgcgccgccc tgcagcgcgg ccaccacgcg gtgatcgacg
     2701 tcggcgcggt aggcgtggat ccggtcgagc ccgggccggg tgagcatgcc gcgccgcggg
     2761 cggggcacgc gcgggccgac cgactcgtag tagctgttga acaggaactc gaacgccgga
     2821 tcgaacggct cgccgccgag cacgaacgcc tcgaagaacc acgtggtgtg cgcgaggtgc
     2881 cacttggcgg ggctggcgtc cggcatcggc tggacgacct gatcctcggc cgacagcggg
     2941 gcgcacagcg cgatcgtgcg agcgcgcact gcgaggaacc gctcccgcag cgactcggcg
     3001 cgcgcggagt ccgtcggcgc gctgcggtgg gctcgtgagg tccgtgacgg caccatggcg
     3061 gcccaaccat ggcccgatcc gccccgcgag gcgaccacga tcgtgcggat cgtgaccgga
     3121 ggctgtcagg tttgcgaccg ggagcggcgc aacctgcgga atgccggcga gcaagatgag
     3181 gaatatcgat caggccttat cagccgagat ccattgcagc ggtcagtgaa agatg
//
LOCUS       DASVOR010001062         4423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_11339,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001062 DASVOR010000000
VERSION     DASVOR010001062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11339"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..396
                     /locus_tag="VF516_20380"
     CDS             28..396
                     /locus_tag="VF516_20380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110104.1"
                     /translation="MEAMLPVSDSVRHTHASPRSAVGFLLHAAALTRQQLGNRINLAM
                     PGVSCTVGEQIAALGRVAGPATVARIKRVPDELVIRIVAGWPERVNAQRALSLGFKAE
                     TDFEDIVRAHIEDELGGKVS"
     gene            complement(488..1723)
                     /locus_tag="VF516_20385"
     CDS             complement(488..1723)
                     /locus_tag="VF516_20385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110105.1"
                     /translation="MLGSLVACAPTYAPMHVTVETGAAPQLLAVRDATSTGWRGVATA
                     GTTAFDLVVSGPYEVVVRCAPRFEGVESVTVYARSPSDPPLLDASCAAPVPPSLTVSG
                     TLVEPGQIALGHTGDFSENPDWSFALQLAPGTYDLVLFQDESFSTPEQIALRRDLKLT
                     GDLDLGTLDVAQEPGHAVVETRFAAPSLLPGEQLSPSLELRTATTTAFLPASLELPLG
                     KDVPDAWRVPLVPDAALRPTDRQTVSLAALSTSTIDGIIHGSLRLAARDIRVGDTAGL
                     TLPEPLAPIAFDMARHRITAAWSSLGNYTDVLLTYLSQGSGNLPGRGYALLLSRNYIE
                     DVGMAGATLDLTGVPGLSEDWQIDPARWALRELDVIQGDLDDPRAAWGVLEAPMLQPV
                     TVPRRGTMIRWSSPVHRML"
     gene            complement(1843..2508)
                     /locus_tag="VF516_20390"
     CDS             complement(1843..2508)
                     /locus_tag="VF516_20390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110106.1"
                     /translation="MLAVLAGCSGDVAPDWQLSHDRVIAVRATPPRIVPGEVAQIDAL
                     LGHLGQPPDDVDPDTAEVVSPARLAGALARSAHWRVTAPGDDQLAAARSELGLAAGAP
                     VPLQLRVGFTDLGQTALKIVWLGQHTDNPVIDPVTIDGRDGLAEAQLTVPAGVDVPLA
                     VDFGATYNLNWLTSCGTMHDFDLAMAHLRVEPEDPHAGTLAIVVRDALGGVAWHLWPI
                     TAQ"
     gene            complement(2609..>4423)
                     /locus_tag="VF516_20395"
     CDS             complement(2609..>4423)
                     /locus_tag="VF516_20395"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8110107.1"
                     /translation="PELTGEKIEVTGQAPTPAAGAAELDRQDIQRLPGTGGDVVRALS
                     AMPGVVNQQIPPGYSGVVIRGSSPQDSKVLIDDFEVPILFHPLGIRAVVPAESIETLT
                     FIPGGFDVAYGRSSSGIVQLTTRPGSEARSTQAEVSLIDGGLIAQGPLGKHTRYMLAL
                     RRSVIDFVLPRVIPSSVDLSLTTVPQYWDEQLRIDHELNPAWNLSVSSVGTDDIFELF
                     TTKEDAPSKRFFTRTRFLRLTGAAKYHDGEWQASLALSGMLSEIDAEVGLYQRLDVPT
                     SLVTPRAVVTRAMREAIGLRDVVWSSGAEAQIGHSSVDIALPLERREGEPLPPYDPKD
                     TTTHFRGAVWFPDFAAWTSVAATFDPRIRLTLGARADYFGRPHELAVQPRSELQVKLT
                     DTLTSRFSAGMYRRPPEFQSELLDPHVRSERSQQLTTGLEYTPILGVRLQGTLYYTDR
                     TDLITHNSDGTLDNDGRGTSKGVELLAMVHGGAWFGWFGYSYSNSVRVDMPGGPQRLF
                     DYDQPHNLNVAASWKRGPWQLGGRFTLYSGCPTRRWSAPSSTAIATCTSRRTARSTRR
                     ARRSTTRSTSASTTRGGGGRRPWSPTSMSRMSISTAAS"
BASE COUNT          654 a         1588 c         1560 g          621 t
ORIGIN      
        1 ggcatcatcc gcgagccgct cgccggcatg gaggcgatgc tgccggtgtc ggacagcgtg
       61 cggcatacgc atgccagccc gcggtccgcg gtcggtttcc tgctgcatgc cgcggcgctg
      121 acgcgccagc agctcggcaa ccggatcaac ctcgcgatgc ccggcgtcag ctgcaccgtc
      181 ggcgagcaga tcgcggccct cggccgcgtc gctggtccgg ccaccgtcgc gcgcatcaag
      241 cgcgtgcccg acgagctggt gatccggatc gtcgcgggat ggcccgagcg cgtgaacgct
      301 cagcgcgcgt tgtcgctcgg cttcaaggcg gaaacggatt tcgaggacat cgtccgcgcc
      361 cacatcgagg acgagctcgg cggcaaagtg agctaggcgc gggccgccgg cgcggccgct
      421 gccacgcgat ctgctcggag gtcgagcgca tgctctggct cgacgtgctc cacgccgatc
      481 tctcgcccta caacatcctg tggaccggcg aggaccatcg gatcatcgtt cccctcctcg
      541 gcaccgtcac cggctgcagc atcggtgcct cgaggacacc ccatgccgcg cgagggtcgt
      601 cgaggtcccc ctggatcacg tcgagctcgc gcagggccca gcgcgccggg tcgatctgcc
      661 agtcctcgga gagaccgggg acacccgtca ggtcgagcgt cgcgcccgcc atccccacgt
      721 cctcgatgta gttgcggctc agcagcagtg cgtagccccg ccccggcagg ttgccggagc
      781 cctggctcag gtacgtcagc aagacgtcgg tgtagttccc cagcgaggac cacgccgcgg
      841 tgatccggtg ccgggccatg tcgaacgcga tcggcgcaag cggttccggc aaggtcaacc
      901 cggcggtgtc gcccacgcgg atgtcgcgcg cggcaaggcg gagactcccg tggatgatcc
      961 cgtcgatcgt gctcgtcgag agcgccgcca gcgagacggt ctgccgatcg gtgggtcgca
     1021 gtgcggcgtc cggaacgagc ggaacgcgcc atgcatcggg cacgtccttc cccaggggga
     1081 gctccagcga cgcgggaagg aacgccgtcg tggtggcggt gcggagctcc agcgacggac
     1141 tcagctgctc accgggcaga aggctcgggg cggcgaaccg ggtctcgacc acggcgtggc
     1201 cgggctcctg cgcgacatcg agcgtgccga gatcgaggtc cccggtgagc ttcagatcgc
     1261 gtcgcagcgc gatctgctcg ggtgtcgaga acgactcgtc ctggaacagc acgagatcgt
     1321 aggttccggg cgcgagctgc agcgcgaagc tccagtcggg gttctcgctg aagtccccgg
     1381 tgtggcccag cgcgatctgc cccggctcca ccagggtgcc gctcacggtg agcgagggcg
     1441 gcaccggcgc ggcgcaggaa gcgtccagca gcggtggatc ggacggcgag cgcgcgtaga
     1501 ccgtgacact ctccacgcct tcgaagcgcg gggcgcagcg cacgaccacc tcgtacgggc
     1561 ccgacaccac gagatcgaac gcggtcgtcc cggcggtggc cacgccgcgc cagcccgtgc
     1621 tcgtcgcatc gcgcaccgcg agcagctgcg gtgctgcccc ggtctcgacc gtgacgtgca
     1681 tcggggcgta cgtcggggcg cacgcgacga gggaacccag cacggcgacg caagcaacct
     1741 tcatcgagcc ctccctgcga accagcggcg ttggtgaccg ctggttgtcg gagactgtac
     1801 acgagtggcc gcggtgaggg agccgtccgc atccgccggc cgtcactgcg ccgtgatcgg
     1861 ccacaggtgc caggcgaccc cgccgagcgc gtcgcggacc acgatcgcca gcgtgccggc
     1921 gtgcggatcc tcgggctcga cccgcaggtg cgccatcgcg agatcgaagt cgtgcatcgt
     1981 gccgcacgag gtcagccagt tgaggttgta ggtcgcgccg aagtcgacgg cgagcgggac
     2041 gtcgacgccg gccggcacgg tcagctgcgc ctcggcgagc ccgtccctgc cgtcgatcgt
     2101 caccggatcg atcaccgggt tgtcggtgtg ctggcccagc cagacgatct tgagcgcggt
     2161 ctggcccaga tcggtgaacc cgacgcgcag ctgcagcggg accggcgcgc cggcggccag
     2221 gccgagctcg ctgcgcgccg ccgccagctg atcgtcgccc ggcgcggtca ccctccagtg
     2281 cgccgaccgg gccagcgccc cggcgaggcg ggccggcgac accacctcgg cggtgtcggg
     2341 gtcgacgtcg tccggcggct ggcccaggtg gccgagcagc gcgtcgatct gcgcgacctc
     2401 gcccgggacg atccggggcg gcgtggcgcg caccgcgatc acccggtcat gcgacagctg
     2461 ccagtccggg gcgacatcgc ccgagcaacc ggcgagcacc gcgagcaccg ccgcccgcct
     2521 catagcaccg ctcgcagccc ggccgacggg atgaacggga tcgactcgaa cgcgctgcgc
     2581 tgggtgtaat cgtagttgta gaaataggtc acgatgctgc ggttgaaata gacattctgg
     2641 acatcgaggt aggcgaccat ggccgccggc ccccacctcc acgcgtagtc gacgcggagg
     2701 tcgacctcgt ggtggatcgg cgcgcgcgcc gagttgatcg cgccgtacgt cgggatgtgc
     2761 aggttgcgat cgctgtcgag ctcggcgctg accaccggcg tgtagggcag cccgagtaca
     2821 gcgtgaaccg cccgccgagc tgccaagggc cgcgcttcca gctcgccgcc acgttgaggt
     2881 tgtgcggctg gtcgtagtcg aacagccgct gcggaccgcc cggcatgtcg acccgcaccg
     2941 agttcgagta cgagtagccg aaccagccga accacgcccc gccgtgcacc atcgcgagca
     3001 gctcgacgcc cttgctggtg ccgcggccgt cgttgtcgag cgtgccgtcg gagttgtggg
     3061 tgatcagatc cgtgcgatcg gtgtaataca gcgttccctg cagccgcacc ccgaggatcg
     3121 gcgtgtactc cagcccggtg gtcagctgct gcgagcgctc cgaccggacg tgcgggtcga
     3181 gcagctccga ctggaactcc ggcgggcggc ggtacatccc cgcgctgaac cgcgacgtca
     3241 gcgtgtcggt cagcttgacc tgcagctcgc tgcgcggctg gaccgcgagc tcgtggggcc
     3301 ggccgaagta gtcggcgcgc gcgccgagcg tcagccggat ccgcggatcg aaggtcgccg
     3361 cgaccgacgt ccacgccgcg aagtcgggga accacaccgc gccgcggaag tgcgtcgtcg
     3421 tgtccttcgg atcgtagggc ggcagcggct cgccctcgcg gcgctccagc ggcagcgcga
     3481 tgtcgaccga cgagtgcccg atctgcgcct cggcgcccga gctccacacc acgtcgcgca
     3541 gcccgatcgc ctcgcgcatc gcgcgcgtca ccaccgcgcg cggcgtgacc agcgacgtcg
     3601 gcacgtcgag ccgctggtac agcccgacct ccgcgtcgat ctcggacagc atccccgaca
     3661 gcgcgaggct cgcctgccac tcgccgtcgt ggtacttcgc cgcgccggtc agccgcagga
     3721 accgggtccg ggtgaagaac cgcttcgacg gcgcgtcctc cttggtcgtg aacagctcga
     3781 agatatcgtc ggtgccgacg ctcgacaccg acaggttcca cgcagggttc agctcgtgat
     3841 cgatccggag ctgctcgtcc cagtactgcg gcaccgtggt cagcgacagg tcgacgctcg
     3901 acgggatcac ccgcggcagc acgaagtcga tcaccgagcg gcgcagcgcc agcatgtacc
     3961 gcgtgtgctt gccgagcggc ccctgcgcga tcagcccgcc gtcgatcagc gagacctcgg
     4021 cctgcgtgct gcgcgcctcg ctgcccggcc gcgtcgtcag ctggacgatc cccgacgacg
     4081 accggccgta cgccacgtcg aacccgcccg ggatgaacgt cagggtctcg atcgactcgg
     4141 ccgggaccac cgcgcggatc ccgagcgggt ggaacaggat cggcacctcg aagtcgtcga
     4201 tcagcacctt ggagtcctgc ggcgacgagc cgcggatcac cacccccgag tagcccggcg
     4261 ggatctgctg gttcaccacg ccgggcatcg cgctcagcgc gcgcaccacg tcgccgccgg
     4321 tccccggcag gcgctggatg tcctggcgat cgagctccgc ggcaccggcc gccggcgtcg
     4381 gcgcctggcc ggtgacctcg atcttctcgc ccgtgagctc ggg
//
LOCUS       DASVOR010001063         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_566243,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001063 DASVOR010000000
VERSION     DASVOR010001063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566243"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1538)
                     /locus_tag="VF516_20400"
     CDS             complement(<1..>1538)
                     /locus_tag="VF516_20400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110108.1"
                     /translation="PPGRTARYQLSLADIRLDRLAQLPAQWARRDFVANTLELELHAR
                     TLPCATTAAIAPELDAGAELHLAGELFSYWDRPYDGAWNLKLDAKNLGPTVRSCIHPM
                     VGGDQLNGTISLTGPFVAQPAVGLDLTGVDVDVPLGKTQEPLRLTLAELHGKIDLVND
                     EGYIEKTKALVRGGKEPGEVDLSATFGLKPLYSNAQVEIVKAIDVGRFLPRSVGPLGK
                     FLQGRLRAIGDVDEGFELREFDLALGATPSEKALRVHHGRVWTKNKFGKIQIDSVYAD
                     AGKSHAELNGAIDVDKNDMDLQIDGNFPDLDVWLQRFGLPALVKSAGGGVIKIQGPLT
                     GRSPTVRVDTTLAGVPCVDQLRLENLVVRGDTIDIQKLSSPGLGGQLTGTGVIRLGGA
                     APVIERLQLTGSRLDASRLCGIKGVKGTIDEVDVDLHGDINPRRTVLDWLGLGQVYAR
                     ADHLSVLDDRYSAISACLNRRDDQRCRARPASANAEDRGQCEDGKRAAAAGGSGFCVV
                     ASAT"
BASE COUNT          197 a          538 c          564 g          239 t
ORIGIN      
        1 ggtcgccgag gcgaccacgc agaacccgct gccaccggcc gccgcggcgc gcttgccgtc
       61 ctcgcactgc ccgcggtcct cggcgttggc cgacgccggc cgcgcccggc agcgctggtc
      121 gtcgcggcgg ttgaggcacg ccgagatcgc cgagtagcgg tcgtcgagca cgctcaggtg
      181 gtcggcgcgc gcgtagacct ggcccaggcc gagccagtcg agcacggtgc gccgcgggtt
      241 gatgtcgccg tgcaggtcga cgtcgacctc gtcgatcgtg cccttgacgc ccttgatccc
      301 gcacagccgc gatgcgtcga gccggctgcc ggtcagctgg agccgctcga tcaccggggc
      361 cgcgccgccg agccggatca cgccggtgcc ggtcagctga ccgccgagcc ccggcgagct
      421 cagcttctgg atgtcgatcg tgtcgccgcg aaccaccagg ttctcgagcc ggagctggtc
      481 gacgcacggc acgccggcga gcgtggtgtc gacccggacc gtcgggctgc gcccggtcag
      541 cgggccctgg atcttgatca cgccgccgcc ggcgctcttg accagcgcgg gcaggccgaa
      601 gcgctggagc cagacgtcga gatcggggaa gttgccgtcg atctgcaggt ccatgtcgtt
      661 cttgtcgaca tcgatcgcgc cgttgagctc ggcgtggctc ttgccggcgt cggcgtagac
      721 gctgtcgatc tggatcttgc cgaacttgtt cttggtccac acccggccgt ggtggacgcg
      781 gagcgccttc tcgctcggcg tcgcgcccag cgcgagatcg aactcgcgga gctcgaagcc
      841 ctcgtcgacg tcgccgatcg cgcgcagccg gccttgcagg aacttgccga gcgggccgac
      901 cgagcggggc aggaaccggc cgacgtcgat cgccttgacg atctcgacct gcgcgttgga
      961 gtacagcggc ttcaagccga acgtcgccga cagatcgacc tcgccgggct ccttgccgcc
     1021 gcgcaccagc gccttggtct tctcgatgta gccctcgtcg ttgaccaggt cgatcttgcc
     1081 gtggagctcg gcgagcgtca gccgcagcgg ctcctgggtc ttgcccagcg ggacgtcgac
     1141 gtcgaccccg gtcagatcga gcccgaccgc gggctgggcg acgaacgggc cggtcagcga
     1201 gatcgtgccg ttcagctgat cgccgccgac catcgggtgg atgcacgacc gcacggtcgg
     1261 cccgaggttc ttggcgtcga gcttgaggtt ccacgcgccg tcgtacggcc ggtcccagta
     1321 gctgaacagc tcgcccgcga ggtggagctc ggcgccggcg tccagctccg gggcgatcgc
     1381 cgccgtggtc gcgcagggca gggtgcgggc gtggagctcg agctccaggg tgttggccac
     1441 gaagtcgcgg cgcgcccatt gcgcgggcag ctgggcgagc cggtcgagcc ggatgtcggc
     1501 gagcgacagc tggtaacgcg cggtgcgtcc gggcggcg
//
LOCUS       DASVOR010001064         2444 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_448856,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001064 DASVOR010000000
VERSION     DASVOR010001064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_448856"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2444
                     /locus_tag="VF516_20405"
     CDS             <1..>2444
                     /locus_tag="VF516_20405"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX8110109.1"
                     /translation="LALTRLLDAYGVRPDLVMGHSLGEYGALVAAGVLPFAHALEAVS
                     ARGREMTQVAVADPGWMVAVTAPLSEIEAVVAATEGYVVVANLNSRKQAVIGGETAAV
                     ERAMQALVARGHACVRLPVSHAFHTRIVAPASEPLRRVLDRLDVRPPRLPVVSNVTGA
                     LYPGDGGRDAIIDLLARQVASPVQFVQGLETLYAEGVRTFVEVGPKRALAGLADDTLG
                     DRPGVIALATNHPKLGDAVAFNHALCGLYAAGFGAAAPAELPGALRVSSGAARHAEPP
                     AMPVVITGAALGLPGTPRVFDDGNLARVLRGDTLIDVVPARHRRAMVDRHVTRVVKGE
                     DGSGAFQRIDDPDDVIRLAGRGGALDLEAEFGVTAERIAALDVTTQLAIGAGLDALRD
                     AGIPLVRHYKTTSKGTRLPDRWGLPEALRDDTGVIFASAFPGYDAFAADLERYHRDAA
                     RRDQIALLDRVLAGLPADAPLRAELAYRRDELTAEQARDGYEFDRRFLFRILAMGHSQ
                     LAELIGARGPNTQVNAACASTTQAIALAEDWIRAGRCRRVIVVAGDDVTSDHLLAWIG
                     AGFLASGAAATDDVVERAALPFDRRRHGMIMGMGAAAIVVEHPDAARERGLAPIAEVL
                     GTAVANSAFHGTRLDVHHIAQVMERVVRAAEATAGVTRHAMAPELMFMSHETYTPARG
                     GSASAEVHALREVFGDAADRIVIANTKGLTGHAMAVGIEDVVAIKALETGVVPPIPNH
                     REVDPELGALNLSHGGPYPIRYALRLGAGFGSQISMSLLRWTPPPDGAHRPVAALGRD
                     YRVHDRAAWNAWLARLA"
BASE COUNT          296 a          957 c          887 g          304 t
ORIGIN      
        1 ctcgcgctga cccggctgct cgacgcctac ggcgtgcgcc ccgacctggt gatgggccac
       61 agcctcggcg agtacggcgc gctggtcgcc gcgggcgtgc tgccgttcgc gcacgcgctc
      121 gaggcggtca gcgcgcgcgg ccgcgagatg acgcaggtcg cggtcgccga cccgggatgg
      181 atggtcgcgg tgaccgcgcc gctgtccgag atcgaggcgg tggtcgcggc gaccgagggc
      241 tacgtcgtgg tcgccaacct caacagccgc aagcaggcgg tgatcggcgg cgagaccgca
      301 gcggtcgagc gcgcgatgca ggcgctggtc gcgcgcggcc acgcgtgcgt ccggctgccg
      361 gtcagccacg cgttccacac ccggatcgtc gccccggcga gcgagccgct gcgccgcgtg
      421 ctggaccggc tcgacgtccg gccgccgcgc ctgccggtgg tgtccaacgt caccggcgcg
      481 ctgtaccccg gcgatggcgg gcgcgacgcg atcatcgatc tgctcgcccg ccaggtcgcg
      541 tcgccggtcc agttcgtcca gggcctggag accctgtacg ccgagggcgt gcgcacgttc
      601 gtcgaggtcg gacccaagcg cgcgctcgcc ggcctcgccg acgacacgct cggcgatcgc
      661 ccgggcgtca tcgcgctggc gaccaaccac cccaagctcg gtgacgcggt cgcgttcaac
      721 cacgcgctgt gcggcctgta cgccgccggc ttcggcgccg cggcaccggc cgagctcccg
      781 ggggcgctgc gcgtttcctc gggcgcggct cgccacgcgg agccacccgc catgccggtc
      841 gtgatcaccg gcgccgcgct cggcctgccc gggacgccgc gcgtgttcga cgacggcaac
      901 ctcgcgcgcg tgctgcgcgg cgacaccctg atcgacgtcg tgccggcccg ccaccgccgc
      961 gcgatggtcg accgccacgt cacccgcgtg gtcaagggcg aggacggctc cggcgcgttc
     1021 cagcggatcg acgatcccga cgacgtgatc cggctcgccg ggcgcggcgg cgcgctcgac
     1081 ctcgaggccg agttcggcgt caccgccgag cggatcgccg cgctcgacgt caccacgcag
     1141 ctcgcgatcg gcgccgggct cgatgcgctg cgcgacgccg gcatcccgct ggtccgccac
     1201 tacaagacca ccagcaaggg cacgcggctc cccgatcgct ggggcctgcc cgaggcgctg
     1261 cgcgacgaca ccggcgtgat cttcgcgtcg gcgttccccg gctacgacgc gttcgccgcc
     1321 gatctcgagc gctaccaccg cgacgccgcc cggcgcgacc agatcgcgct gctcgaccgc
     1381 gtgctcgccg ggctgccggc cgacgcgccg ctgcgcgccg agctcgccta ccggcgcgac
     1441 gagctcaccg ccgagcaggc gcgcgacggc tacgagttcg atcgccggtt cctgttccgc
     1501 atcctggcga tgggacactc gcagctcgcc gagctgatcg gcgcgcgcgg gcccaacacc
     1561 caggtcaacg cggcgtgcgc cagcaccacg caggcgatcg cgctcgccga ggactggatc
     1621 cgggccgggc ggtgtcgccg cgtgatcgtg gtcgccggcg acgacgtcac ctccgaccac
     1681 ctgctggcgt ggatcggcgc cggctttctg gcgagcggcg cggccgcgac cgacgacgtc
     1741 gtcgagcgcg cggcgctgcc gttcgaccgc cgccgccacg gcatgatcat gggcatgggc
     1801 gccgcggcga tcgtcgtcga gcaccccgac gccgcgcgcg agcgcgggct cgctccgatc
     1861 gccgaggtgc tcggcaccgc ggtcgccaac agcgcgttcc acggcacgcg gctcgacgtc
     1921 caccacatcg cccaggtcat ggagcgcgtc gtgcgcgcgg ccgaggccac cgccggcgtg
     1981 acgcgccacg cgatggcgcc cgagctgatg ttcatgtcgc acgagaccta cacgccggcg
     2041 cgcggcggca gcgcgtcggc cgaggtccac gccctgcgcg aggtgttcgg cgacgccgcg
     2101 gaccgcatcg tgatcgccaa caccaagggc ctcaccggcc acgcgatggc ggtcgggatc
     2161 gaggacgtcg tcgcgatcaa ggcgctcgag accggcgtcg tgccgccgat ccccaaccac
     2221 cgcgaggtcg atccggagct cggcgcgctc aacctgtcgc acggcgggcc gtacccgatc
     2281 cgctacgcgc tgcggctcgg ggccgggttc ggctcgcaga tcagcatgtc gctgctgcgc
     2341 tggacgccgc cgcccgacgg cgcgcaccgc ccggtcgccg cgctcggccg cgactaccgc
     2401 gtgcacgacc gcgcggcgtg gaacgcctgg ctggctcggc tggc
//
LOCUS       DASVOR010001065         2596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390291,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001065 DASVOR010000000
VERSION     DASVOR010001065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390291"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..753)
                     /locus_tag="VF516_20410"
     CDS             complement(139..753)
                     /locus_tag="VF516_20410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110110.1"
                     /translation="MRIQSEGSMIAPRSWVRRALLEALARSRPARSARCAACATVPEI
                     AKLLTSPRRRRDTRIAMARYVPEPGEVELLKELVEPIRGDQRVHGMLILTDRRLVLES
                     VRPSPALRWLFRWFAPILILFRAANELGPLEMTHQIDRDDFDAVEAQGRQMISFHSNG
                     KGYGHISFAVYSMTPINVWQHRMRAWVAGALSAPPGVPARPVGR"
     gene            complement(780..1553)
                     /locus_tag="VF516_20415"
     CDS             complement(780..1553)
                     /locus_tag="VF516_20415"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8110111.1"
                     /translation="MTARVRVAIKTRMAKASSRRQSKPVQQELFRHGGKRKGAGRKPR
                     HGRAGSPHARRPAVKPHHALHVVMRVVPAVGSLRRRAMYKALRDATITAALRERIRIV
                     HVSVQRTHVHMLIEAGDARALARGMQGFEISAARNINTMLGAGGRRRRGPVFTDRYHL
                     EVITTPKRARNALSYILNNWRHHREDQDGPASTWLVDPFSSGISFPDWLELEGKDVMW
                     PIRETYDPLMVRRPQSWLLREGWKRHGMISAREVPGPRR"
BASE COUNT          444 a          885 c          844 g          423 t
ORIGIN      
        1 ggcgccgccg gcccacgcgc cgtcgtcgag ctcgccgagc cgaccgtcgc cgcccccggc
       61 caggccgccg tgatctacgc cggcgaccgc gtgctcgccg gcggctggat cgcacgcgca
      121 tagcgaagcc ccaccgcgtc accgcccgac gggccgggcc ggaacacccg gtggcgccga
      181 cagggccccg gccacccatg cccgcatgcg gtgttgccag acgttgatcg gtgtcatcga
      241 gtacacggca aacgagatgt gcccgtagcc cttgccattg ctgtgaaagc tgatcatctg
      301 ccggccctgc gcctcgaccg catcgaagtc gtcgcgatcg atctgatgcg tcatctcgag
      361 cggtccgagc tcgttggcag ctcggaagag tatgagtatc ggcgcgaacc agcggaacag
      421 ccaccggagg gccggcgagg gccttacgct ctccagcacc agccgccggt cggtgaggat
      481 cagcatgccg tgcacacgct ggtcgcctcg gatcggctcg accagctcct tcaagagctc
      541 gacttccccg ggctctggca cgtaccgcgc catggcgatc cttgtatcac gccggcggcg
      601 cggtgatgtg agcagcttgg cgatctcagg aaccgtcgcg caggcagcgc accgcgcgga
      661 tcgggcagga cgtgagcggg cgagggcctc caggagcgca cgacggaccc aggatcgcgg
      721 tgcgatcatc gagccttcgc tctggatgcg catgcatccg catcgacgca cgcgtctcgt
      781 cagcgacgcg gcccggggac ctcgcgcgcc gagatcattc catggcgctt ccagccctcc
      841 cggagcaacc agctttgcgg ccggcgaacc atcagcggat cgtaggtctc tcgaatcggc
      901 cacatcacat ccttgccctc cagctccagc cagtccggga aggagatacc gctcgagaac
      961 ggatcgacca gccacgtgct ggccggtccg tcctggtcct cgcggtggtg acgccagttg
     1021 ttcaagatat acgacagtgc attccgcgct cgcttcggag tcgtgatcac ctccaggtga
     1081 tagcggtcgg tgaacaccgg cccgcggcgt cggcggcctc cggctccaag catcgtgttg
     1141 atgttccggg cagcggagat ctcgaagcct tgcatgccac gcgcgagcgc cctggcatcc
     1201 ccggcctcga tgagcatgtg cacgtgcgtc cgctgaacgc tgacgtggac gatccggatc
     1261 cgctcgcgca gcgccgccgt gatcgtcgcg tcgcgaagcg ccttgtacat cgcccgccgc
     1321 cgcaggcttc cgactgccgg cacgacccgc atcacgacgt gcagcgcatg gtgcggcttg
     1381 accgccggcc gcctggcatg cggcgacccc gctcgcccgt gcctgggctt gcgtccggcg
     1441 cccttgcgct tgccgccatg ccggaacagc tcctgctgga ccggcttgct ttggcgccgc
     1501 gaactagcct tcgccatgcg agtttttata gccactctga cacgcgccgt caacttgaat
     1561 tctccgaatt aattgtaatg atatgtatcg agagcatcag tattcaatga catgtgaatg
     1621 caatacacag cggctgattc agcgatggcg agcacagttg aggtgcggca tgtacgacgc
     1681 ggcgcgactg cggttccggc gtggccgacg gtcgcaacca actcgtgctg ataactgcgc
     1741 ccgggtagcg acacggctcg gtggttggcc caggcggccg cagaccgcag accataggtc
     1801 ggatccagac ttggcacgcg gcaaaggaac gaggggctac ggacgtagcg cacggaaact
     1861 cgcctgcacc accaagtcta tcgtgaccgc acggcgactg ggtcagggac ctcggaagac
     1921 cgcgcggccg gccctgtgac tggcccgtcg aggctggcca ggcggaccag gacggcccac
     1981 tcacagtgca tcgccccgcg cgggcgctcc ctctccggag aatattgatt tattcgagat
     2041 gtccggaagt ggccgctcgg cgcagatggc agaacttgaa cggcgggacg ccgccgttcg
     2101 ttccccatcg cgtccgcgcg cagagcttct ttcgccacgc gcgcagcacc ggctatcagc
     2161 gcgcctgcgt cgtcgaacca ccgagatgct gcagcgggca gggccagacg tcgcgctgcc
     2221 aaggccgcaa ttcgcaactc catatcaata tacatacata aatccccaag ggtgactggt
     2281 acgggggccc gggctgtggg cagacgcgga gggttgtgag ctgcgtgatg gtgatccgaa
     2341 gcggcttcag cgcaggtcac agcccgaccg tctgtccata gccacgaggg tgttctcctg
     2401 gggacctcat cgtgatctcc cgaataccct gcatgggatc tcgaccgcgg atggaccaga
     2461 ccgatccgtc ggtagaattc gagcagccat cgcctgtccg ccgagcacgg cgaccgggcg
     2521 gctacgcgag cagctcggcg atctcgggga ccgcggcata ggccgcgcgc caggtcggat
     2581 cgggcagggc ggcgag
//
LOCUS       DASVOR010001066         3777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_361499,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001066 DASVOR010000000
VERSION     DASVOR010001066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361499"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VF516_20420"
     CDS             complement(<1..327)
                     /locus_tag="VF516_20420"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX8110112.1"
                     /translation="MSTDAVVIWGELLWDRFPAALGGDRLGGAPANVAWHLAQAGGWA
                     QLVTRVGDDVDGRRALAELDGLVDTSLVQVDPERATGEVTVELEGGEPRYTLHAGRAW
                     ERIACTD"
     gene            359..1861
                     /locus_tag="VF516_20425"
     CDS             359..1861
                     /locus_tag="VF516_20425"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEX8110113.1"
                     /translation="MPGTPERPKLEDLYGGSLALLTDLYQLTMACGYWKAGTADREAV
                     FHLTFRRAPFGGGYAIAAGIASALDYLRNLTYTPDDLAYLATLRDGEGARLFPAGFLD
                     YLERFEFGCTVDAAPEGSLVFGHEPIVRVRGPIAHGQLVETALLTLVNFETLIATKAS
                     RVVQAARGAPVIEFGLRRAHGIDGGLSASRAAYLGGCLGTSNVLAGKRFGIPVFGTHA
                     HSWVMFHGDELAAFRAYAEAMPGNTTLLVDTYDSLDGVRHAIAVGRELRARGHELAGI
                     RLDSGDLAYLSIEARRMLDEAGFPDAKIVASNDLDEVVIGSLLQQGARIDTYGVGTRL
                     VTGYDQPALGGVYKLGGARDEHGAWRDAIKLSEQPTKITNPGNLAVRRLRKGGELVAD
                     IIYDSEAGWQAPLLHDIEDPFRPAWAPVFDEDSDLLVTYLRLGRPVRAPDSLADARAR
                     AAGELDQLSPRARRFLNPQPYPVGLDPHVHARKQQLIALARAPLSPAAPR"
     gene            1911..2828
                     /gene="prs"
                     /locus_tag="VF516_20430"
     CDS             1911..2828
                     /gene="prs"
                     /locus_tag="VF516_20430"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX8110114.1"
                     /translation="MTAPIVFSTQAYDYLGRAMAAATGWERGVVARKTFPDGEHYLRI
                     ESDPADRDVVLVGGTIDDVSTLELYDLACGLVTGGAYRLRIVMPFYGYSTMERSARPG
                     EVVTAKTRARLLSSVPMASRGTQVFLLDLHVEAIAHYFEGGIRPVHIQGKPLVIAAAA
                     RLGGSDFVLACTDAGRAKWVESLANDMNVAAAFVYKRRHDDATAVTGVSAQVAGKRVV
                     IYDDMIRTGGSLLSAARAYRDAGAVAIDAIATHGVFPADSLARIAGSGLLGRIVVTDS
                     HPRAVAYQSEFLEVASTATLLVEHLISNR"
     gene            2847..>3777
                     /locus_tag="VF516_20435"
     CDS             2847..>3777
                     /locus_tag="VF516_20435"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX8110115.1"
                     /translation="MRLVKVAVACVNQTPLAWDDNLAHLRTAIAQARAEGVTVLCLPE
                     LAITGYGCEDAFFMHGLLDTAFAQLDALAAETGGMVVAVGLPVYHEKALYDAGALLAD
                     GEIVGFVGKQFLAADGIHYEPRWFKPWPAGEVDVLERVTPDGTIRRFPIGDLVFDLGD
                     LRIGFEICEDAWVANRPGVDLALRGVDLIANPSASHFAFGKFGVRQRFVVEGSRAFGV
                     TYLYANLMGNEAGRVVYDGGGLIASSGELCARGRRFSFHDVELTCAVVDIDANRPGAD
                     LALRGVDLLLNPSASHFGFGKFAVRQRFVVEGSR"
BASE COUNT          504 a         1389 c         1347 g          537 t
ORIGIN      
        1 gtcggtgcac gcgatgcgct cccacgcccg cccggcgtgc agggtgtagc gcggctcgcc
       61 gccctcgagc tcgaccgtga cctcgccggt cgcgcgctcg gggtcgacct ggaccagcga
      121 ggtgtcgacc aggccgtcga gctcggccag cgcgcggcgg ccgtcgacgt cgtcgccgac
      181 ccgggtcacc agctgggccc acccgccggc ctgggcgagg tgccacgcga cgttggccgg
      241 ggcgccgccc aggcgatcgc cgccgagcgc cgcggggaag cggtcccaca gaagctctcc
      301 ccagatgacg accgcgtcgg ttgacatcgc gctgcttagt gtaaataata caccaaaggt
      361 gcccggaacc ccggaacgtc cgaaactcga agatctgtac ggtggctcgc tcgcgctgct
      421 caccgacttg tatcagctga ccatggcctg cggctactgg aaggccggga cagccgatcg
      481 cgaggcggtg tttcacctga cgttccgtcg agccccgttc ggcggcggct acgcgatcgc
      541 ggcggggatc gcgtcggcgc tggattacct gcgcaacctg acgtacacgc ccgacgacct
      601 ggcgtacctg gcgaccctgc gcgacggcga gggcgcgcgg ctgttcccgg ccggcttcct
      661 cgactacctc gagcgcttcg agttcgggtg cacggtcgac gccgcgcccg agggcagcct
      721 ggtgttcggc cacgagccga tcgtgcgggt gcgcgggccg atcgcgcacg gccagctggt
      781 cgagaccgcg ctgctcacgc tggtcaactt cgagaccctg atcgcgacca aggcgtcgcg
      841 cgtggtccag gccgcgcgcg gcgcgccggt gatcgagttc gggctgcgcc gcgcccacgg
      901 catcgacggc gggctgtcgg cgtcgcgcgc ggcgtacctc ggcggctgcc tcgggacctc
      961 gaacgtgctc gccggcaagc ggttcgggat cccggtgttc ggcacccacg cccacagctg
     1021 ggtgatgttc cacggcgacg agctggcggc gttccgggcc tacgccgagg cgatgcccgg
     1081 caacaccacg ctcctggtcg acacctacga ctcgctcgac ggcgtgcgcc acgcgatcgc
     1141 ggtcggccgc gagctgcgcg cgcgcggcca cgagctcgcc gggatccggc tcgactccgg
     1201 cgacctcgcg tacctgtcga tcgaggcccg ccggatgctc gacgaggccg gcttccccga
     1261 cgcgaagatc gtcgcgtcga acgacctcga cgaggtggtg atcggctcgc tgctccagca
     1321 gggcgcgcgg atcgacacct acggggtcgg cacccggctg gtcaccggct acgatcagcc
     1381 cgcgctcggc ggggtgtaca agctcggcgg ggcgcgcgac gagcacggcg cgtggcgcga
     1441 cgcgatcaag ctgtcggagc agccgaccaa gatcaccaac ccgggcaacc tcgcggtccg
     1501 ccggctgcgc aagggcggcg agctggtcgc cgacatcatc tacgacagcg aggccggctg
     1561 gcaggcgccg ctgctccacg acatcgagga cccgtttcgc ccggcgtggg cgccggtgtt
     1621 cgacgaggac agcgacctcc tggtcaccta cctccggctc ggccggccgg tccgcgcgcc
     1681 ggacagcctg gccgacgccc gggcgcgcgc ggccggcgag ctcgaccagc tgtcgccgcg
     1741 cgcccgccgg ttcctcaacc cgcagcctta cccggtgggc ctggacccgc acgtccacgc
     1801 ccgcaagcag cagctgatcg cccttgcccg cgcgcccctg tcgcccgcgg cgccgcggtg
     1861 aggccatact ggatggagca cccgactgcc cgacgcaaga ccccggagcc atgaccgccc
     1921 cgatcgtgtt ctcgacccag gcctacgact atctcggccg cgccatggcg gcggcgaccg
     1981 gctgggagcg cggcgtcgtc gcgcgcaaga cgttccccga cggcgagcac tacctgcgga
     2041 tcgagtccga tccggcggac cgcgacgtcg tgctggtcgg cggcacgatc gacgacgtct
     2101 ccaccctgga gctctacgac ctggcgtgcg gcctggtgac cggcggggcg taccggctgc
     2161 gcatcgtgat gccgttctac ggctactcga cgatggagcg ctcggcccgc cccggcgagg
     2221 tcgtgaccgc caagacgagg gcgcgcctcc tgtcgtcggt gccgatggcc agccgcggca
     2281 cccaggtgtt cctgctcgac ctccacgtcg aggcgatcgc gcactacttc gagggcggga
     2341 tccggccggt ccacatccag ggcaagccgc tggtcatcgc ggcggcggcc cggctcggcg
     2401 gcagcgactt cgtgctggcg tgcaccgacg ccgggcgcgc caagtgggtc gagtcgctcg
     2461 ccaacgacat gaacgtcgct gccgcgttcg tctacaagcg gcgccacgac gacgcgaccg
     2521 cggtgaccgg ggtgtcggcc caggtcgccg gcaagcgggt cgtgatctac gacgacatga
     2581 tccggaccgg cggctcgctg ctctcggccg cgcgcgccta ccgcgacgcc ggggcggtgg
     2641 cgatcgacgc gatcgcgacc cacggggtgt tcccggccga ctcgctggcg cggatcgccg
     2701 gcagcgggct gctcggccgg atcgtggtca ccgacagtca cccccgcgcg gtggcgtacc
     2761 agagcgaatt cctggaggtc gcgagcaccg cgaccctgct cgtggagcat ctgatctcca
     2821 accgctgaac ggtcgaggat gcccgcatga gactcgtcaa ggtcgccgtc gcctgcgtca
     2881 accagacccc gctcgcctgg gacgacaacc tcgcccacct gcggaccgcg atcgcgcagg
     2941 cgcgcgccga gggcgtgacc gtgctgtgcc tgcccgagct ggcgatcacc gggtacggct
     3001 gcgaggacgc gttcttcatg cacggcctcc tggacaccgc gttcgcccag ctcgacgcgc
     3061 tggcggccga gaccggtggc atggtggtcg cggtcggcct gccggtctac cacgagaagg
     3121 cgctgtacga cgccggcgcg ctgctcgccg acggcgagat cgtcgggttc gtcggcaagc
     3181 agttcctcgc cgccgacggc atccactacg agccgcggtg gttcaagccg tggccggccg
     3241 gcgaggtcga cgtgctggag cgggtcaccc ccgacggcac gatccggcgg ttcccgatcg
     3301 gcgacctcgt gttcgacctc ggcgacctcc ggatcggctt cgagatctgc gaggacgcgt
     3361 gggtcgccaa ccgccccggg gtcgacctcg cgctgcgcgg cgtggatctg atcgccaacc
     3421 cgtcggcgag ccacttcgcg ttcggcaagt tcggggtccg ccagcgcttc gtcgtcgagg
     3481 gctcgcgcgc gttcggcgtg acctacctgt acgccaacct gatgggcaac gaggccggcc
     3541 gggtggtcta cgacggcggg ggcctgatcg cctccagcgg cgagctgtgc gcgcgcggcc
     3601 ggcggttctc gttccacgac gtcgagctga cctgtgcggt ggtggacatc gacgccaacc
     3661 ggcccggcgc cgacctggcg ctgcgcggcg tcgatctcct gctcaacccg tcggccagcc
     3721 acttcgggtt cggcaagttc gcggtgcgcc agcgcttcgt ggtcgagggc tcgcgcg
//
LOCUS       DASVOR010001067         2832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_186362,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001067 DASVOR010000000
VERSION     DASVOR010001067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2832
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186362"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="VF516_20440"
     CDS             <1..658
                     /locus_tag="VF516_20440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8110116.1"
                     /translation="TPAFASSRAVWLERGGVIAIPNLRGGGEYGEDWHRAGMMLVKQN
                     VCDDFIAAAEYLIAERWTSHDHLAISGGSNGGLLVGAAVTQRPDLFKAVICAVPLLDM
                     LRYHLFGSGKTWVPEYGSAEDDAQFKALYQYSPYRVAVDAGARGYPAVLFDSADHDDR
                     VDPLHARKLAAVLQAAQTSDAPILLRIERNAGHGGADLVKQQVERSADQMAWLETLLR
                     "
     gene            886..1299
                     /locus_tag="VF516_20445"
     CDS             886..1299
                     /locus_tag="VF516_20445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110117.1"
                     /translation="MTLENTFTATSEITESAAPAWAAMPEAMPEETMIDDAGDDAGDD
                     AGDDAGDPPCTGAGLARAPRGERQAVVIDEFDPTLSFRDAKERAIGLWERWYVAELLR
                     HTRGNLSEAARVAHSDRSHLRKLVRKHLRSAITPP"
     gene            1296..2099
                     /locus_tag="VF516_20450"
     CDS             1296..2099
                     /locus_tag="VF516_20450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110118.1"
                     /translation="MKHPALVATLVLAAGCPHGGTPQPSGPAPTVAEVVDRLTKAKDE
                     LHSFTGEAVMEYWLGNDRFKGDVLAMGETGSKVRVAALSPAGGSTVAEMACDGSKFVS
                     VNYQNNCVLTGPCNKQSIATFFGIELAPDDFLHLALGAPPLIAQPTGGVTWDADKGAY
                     QIKLSGADGTQTLAIAVHDQHWDVVESERVGADGKPEWTVKNAAFTDVKDPEGKPHRV
                     PGKSRLTTPSNQKADLTVEWHERTVNLTIDPEKFKLTAPAGLPTCAPPR"
     gene            complement(2104..2355)
                     /locus_tag="VF516_20455"
     CDS             complement(2104..2355)
                     /locus_tag="VF516_20455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110119.1"
                     /translation="MATRTFHVQVGDQVFLEEGGEEIGAVRRVAKDHVVIYIEGAGDF
                     TLTGPEIAAAHDGKLRLDPTRLDPALLSAARSAHDHETE"
     gene            2548..>2832
                     /locus_tag="VF516_20460"
     CDS             2548..>2832
                     /locus_tag="VF516_20460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110120.1"
                     /translation="MTPVRARAVRARLALAAAALWLAGVEALPALHEALHDRLAPHVH
                     TAGAIVTVSFEDTTHRHPDGTIHFATPRTPRRGHPVRDGQPRTRDVAQHAA"
BASE COUNT          432 a         1004 c         1008 g          388 t
ORIGIN      
        1 gacgccggcg ttcgcgagct cgcgcgcggt gtggctcgag cgcggcgggg tgatcgcgat
       61 ccccaacctg cgcggcggcg gcgagtacgg ggaagactgg caccgcgccg gcatgatgct
      121 ggtcaagcag aacgtgtgcg acgacttcat cgccgcggcc gagtacctga tcgccgagcg
      181 ctggacgtcg cacgaccacc tggcgatctc gggcggctcc aacggcggcc tgctggtggg
      241 cgcggccgtc acccagcggc cggacctgtt caaggcggtg atctgcgcgg tcccgctgct
      301 cgacatgctg cgctatcacc tgttcggctc gggcaagacc tgggtgccgg agtacggctc
      361 ggcggaggac gacgcgcagt tcaaggcgct gtaccagtac tcgccgtacc gcgtggcggt
      421 cgacgccggc gcgcgcggct acccggcggt gctgttcgac tccgccgatc acgatgaccg
      481 ggtcgatccg ctgcacgcgc gcaagctcgc cgcggtcctc caggcggcgc agacctccga
      541 tgcgccgatc ctgttgcgga tcgagcgcaa cgccggccac ggcggcgccg acctggtcaa
      601 gcagcaggtc gagcgcagcg ccgaccagat ggcgtggctg gagacgctgc tgcgctgaaa
      661 cgcagcgggt tgcggcgggc aggcgcggcc ggtgcgcggc ggatcccgca tgcggatcgc
      721 tgacttggcc gcgcgcggcg gccgggtgat cgcggctgca ggcagcggac atcacctgca
      781 ggcgcgggcc tgcagtccgc ccggcctgct cgctcgtaac acctggcgcc agcgtggctg
      841 gcccggccct cggggctggt ccatcagctg cagaggattc caccgatgac cctcgagaac
      901 acgtttaccg caacttccga gatcaccgag tcggcggcgc cggcatgggc cgcgatgccg
      961 gaggcgatgc cagaagagac gatgatcgat gacgcgggtg acgacgcggg tgacgacgcg
     1021 ggtgacgacg ccggtgaccc gccgtgcacc ggcgccggac tcgcccgcgc gccgcgcggc
     1081 gagcgccagg cggtcgtgat cgacgagttc gacccgacgc tgtcgttccg cgacgccaag
     1141 gagcgcgcga tcggcctctg ggagcgctgg tacgtggccg agcttctgcg ccacacccgg
     1201 ggcaacctgt cggaggcggc gcgggtcgcg cacagcgatc gcagtcacct gcgcaagctg
     1261 gttcgcaagc accttcggtc tgctatcaca cctccatgaa acaccctgcg ctcgtcgcca
     1321 cgctcgtgct cgccgctggc tgtccgcatg gcggaacgcc gcagccgagc gggccggcgc
     1381 cgacggtcgc cgaggtcgtc gatcggttga ccaaggccaa ggacgagctg cactcgttca
     1441 ccggcgaggc ggtcatggag tactggctgg gcaacgatcg cttcaagggc gacgtgctgg
     1501 cgatgggtga gaccgggtcc aaggtccggg tcgccgcgct gtcgccggcc ggcggtagca
     1561 cggtcgccga gatggcgtgc gacggcagca agttcgtatc ggtcaactac cagaacaact
     1621 gcgtgctgac cgggccgtgc aacaagcagt cgatcgcgac gttcttcggc atcgagctcg
     1681 cgcccgatga cttcctgcac ctcgcgctcg gcgcgccgcc gctgatcgcc cagcccaccg
     1741 gcggcgtgac ctgggacgcg gacaagggcg cctatcaaat caagctgtcc ggcgccgacg
     1801 gcacgcagac cctcgcgatc gcggtgcacg accagcactg ggacgtggtc gagagcgagc
     1861 gggtcggcgc cgacggcaag cccgagtgga cggtcaagaa cgccgcgttc accgacgtca
     1921 aggaccccga gggcaagccg caccgcgtgc ccggcaagtc ccgcctgacc acgccgagta
     1981 accagaaggc cgacctcacc gtcgagtggc acgagcggac ggtcaacctc accatcgacc
     2041 ccgagaagtt caagctcacg gccccggcgg ggctgccgac ctgcgccccg ccgcgctgac
     2101 cggttactcg gtctcgtgat cgtgggccga gcgcgcggcc gagagcagcg ccgggtccag
     2161 cctcgtcgga tcgagccgca gcttgccgtc gtgcgccgcc gcgatctccg gcccggtcag
     2221 cgtgaagtcg ccggcgccct cgatgtagat cacgacgtga tccttggcga cccggcggac
     2281 ggcgccgatc tcctcgccgc cctcctcgag gaagacctga tcgccgacct ggacgtggaa
     2341 cgtgcgcgta gccatcggtg acctccgttg gcaatttgcc acgcggccga tcgcgtgacg
     2401 agcgcaaccg acggattccg ctgcgcatgt agtcgtcgtc cgcgcaggac atgcgccgca
     2461 gtgccatcga tccgagggcc gatccgaggg ccgcgcagcc tcgtagtgtc cattgtgggg
     2521 caactggctc gtgatacaag ggcccggatg acaccggtcc gcgcacgggc agtgcgagcc
     2581 cgcctggcgc tcgcggccgc ggcgctgtgg ctggccggtg tcgaggcgct gcccgcgctc
     2641 cacgaggcgc tgcacgatcg actcgcgccg catgtccaca ccgccggtgc gatcgtcacg
     2701 gtgtcgttcg aggacaccac gcaccgccac cccgacggca cgatccactt cgcgacgccg
     2761 cggacgccgc gccggggcca tccggtgcgc gacggtcagc cgcgcacgag agatgtcgcc
     2821 cagcacgccg cc
//
LOCUS       DASVOR010001068         2079 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_127498,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001068 DASVOR010000000
VERSION     DASVOR010001068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127498"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF516_20465"
     CDS             complement(<1..342)
                     /locus_tag="VF516_20465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110121.1"
                     /translation="MSAQLFQLLAQLDRPGATELVVATGRPVVVRVNASYHPLTAAAV
                     TLPQLMHLLRGTQLAALVPQGEQVGDPVAIDLDGRALRAQFLRQGADVLLRIEHRPPP
                     QPPARPTPSPAR"
     gene            368..1990
                     /locus_tag="VF516_20470"
     CDS             368..1990
                     /locus_tag="VF516_20470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110122.1"
                     /translation="MGRWQRYWFDEGGRWSAAVVRIAIATSVLMTLYRLATLSTVRIP
                     APSELYRPIGIWMMFGRWVPPGEVVTALWVLATGATLLMLVGLWSRASAAVSCAAALA
                     LTSASFSSSQSWSHQYNVVFLAALAFLGARGGDALSLDAWIRRKRARPPLDVPRGYQW
                     SVRLVQFAVALMFAGAALDKLAYGHFTLRWALSDNLRHQLLMHYDLAGIPRTPVADWL
                     LDDVWRFRTAALLNLISQASPILACVFVRRPWVRALAGGFFALETIALGLVVNLWNPH
                     WLPLVAVFIDWEWLAAVFTGRTAAAPPVPDGWTPPRAVHAYVIAFVIYDAVTAIVPTL
                     DQRLNTYPFSAFPMFAKVRARRPYDQHLPYTVPGDQFEALSDRPLDDRARRWIDYQNR
                     KLYMIADPARYRTKLAAILADTRKRFPEFGIHGLRHYLTIFEAPAYPAAARFVRHPVG
                     VMGELDEAGTFRSALGAIDDASVTLRPQHLDTTGAQLVSFLDDDPTPHALPAARAGDR
                     FATGPVDGDPLYVVAIISGTPWLVATRVSHWE"
BASE COUNT          251 a          768 c          736 g          324 t
ORIGIN      
        1 gcgcgctggc gacggcgtgg gccgcgcggg tggctgcggc ggcggccggt gctcgatgcg
       61 cagcaggacg tcggcgccct ggcggaggaa ctgcgcgcgc agcgcccggc cgtcgagatc
      121 gatcgccacc ggatcaccga cctgctcgcc ctgcggcacc agcgccgcga gctgggtgcc
      181 gcggagcagg tgcatcagct gcggcagggt cacggcggcc gcggtcagcg ggtggtacga
      241 ggcgttgacc cggaccacga cgggacgccc ggtcgcgacg accagctcgg tggcgccggg
      301 gcggtcgagc tgtgcaagga gctggaacag ctgcgcactc accagacgcc catggtacta
      361 cgccgcggtg ggccgctggc agcgctactg gttcgacgag ggggggcggt ggtccgccgc
      421 ggtggtccgg atcgcgatcg ccacctcggt tttgatgaca ctgtatcggc tcgcgacgct
      481 gtcgaccgtt cgcatcccgg cgccctccga actctaccga ccgatcggca tctggatgat
      541 gttcggccgc tgggtcccgc cgggcgaggt ggtgaccgcg ctgtgggtgc tcgcgaccgg
      601 cgcgacgctg ctcatgctgg tcgggctgtg gtcgcgcgcc tccgcggcgg tcagctgcgc
      661 cgccgcgctc gcgctgacct cggcgtcgtt ctcgtcgagc cagagctggt cgcaccagta
      721 caacgtcgtg ttcctcgccg cgctggcgtt ccttggggcg cgcggcggcg acgcgctgtc
      781 gctcgacgcg tggatccggc gcaagcgcgc ccggccgccg ctcgacgtgc cgcgcggcta
      841 ccagtggtcg gtccggctgg tccagttcgc ggtcgcgctg atgttcgccg gcgccgcgct
      901 cgacaagctg gcgtacggcc acttcacgct gcgctgggcg ctgtccgaca acctgcgcca
      961 ccagctgctg atgcactacg acctcgccgg gataccgcgc acgccggtcg ccgactggct
     1021 gctcgacgac gtgtggcggt tccgcaccgc cgcgctgctc aacctgatct cgcaggccag
     1081 cccgatcctc gcgtgcgtgt tcgtccgccg gccgtgggtc cgcgcgctgg ccggcgggtt
     1141 cttcgcgctc gagaccatcg cgctcggcct ggtggtcaac ctgtggaacc cgcactggct
     1201 gccgctggtg gcggtgttca tcgactggga gtggttggcc gcggtgttca ccgggcggac
     1261 cgcggccgcg ccgccggtgc ccgacggctg gacgccgccg cgcgccgtac acgcgtacgt
     1321 gatcgcgttc gtgatctacg acgcggtcac cgcgatcgtg cccacgctcg atcagcggct
     1381 caacacctac ccgttctcgg cgttcccgat gttcgccaag gtgcgcgccc ggcggcccta
     1441 cgaccagcac ctgccgtaca ccgtgcccgg cgatcagttc gaggcgctgt ccgatcggcc
     1501 gctcgacgac cgcgctcggc gctggatcga ttatcagaac cgcaagctct acatgatcgc
     1561 cgatccggca cgctatcgca caaagctcgc cgcgatcctc gccgataccc gcaagcggtt
     1621 ccccgagttc ggcatccacg gcctgcgcca ctacctcacg atcttcgaag cgccggccta
     1681 cccggcggca gcgcgcttcg tgcgccaccc ggtcggggtg atgggcgagc tcgacgaggc
     1741 cggcacgttc cgcagcgcgc tcggcgcgat cgacgacgcg tcggtcacgc tacgacccca
     1801 gcacctcgat accaccgggg cgcagctggt cagcttcctc gacgacgatc ccacgccgca
     1861 cgcgctgccc gccgcgcgcg cgggcgaccg cttcgcgacc ggccccgtcg acggagatcc
     1921 gctctacgtg gtcgcgatca tcagcggcac gccgtggctg gtcgcgaccc gcgtttcgca
     1981 ctgggagtga gcgctcagtg caccgcaccc ttggtggtcg gatcgctgac ctcgacggtc
     2041 ggcggggccg ccggccgcgc gctgtcgctg gcgaccgcc
//
LOCUS       DASVOR010001069         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_69042,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001069 DASVOR010000000
VERSION     DASVOR010001069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69042"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            193..615
                     /gene="ruvA"
                     /locus_tag="VF516_20475"
     CDS             193..615
                     /gene="ruvA"
                     /locus_tag="VF516_20475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEX8110123.1"
                     /translation="MTVKNVGPSTAIAILSGSSPRDIATLIAREDVVGLTRIKGIGKK
                     TAELLVVELRDKCELLLLSWSATAEGGVRSVAIPAGAARPRSSVPLRSPVIADVMTAL
                     VAMGWRPAEAEAAVSDLQVGDNASLESLLRQALRNMPR"
     gene            630..1715
                     /gene="ruvB"
                     /locus_tag="VF516_20480"
     CDS             630..1715
                     /gene="ruvB"
                     /locus_tag="VF516_20480"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEX8110124.1"
                     /translation="MPRKKHTDPPGEPPDAAREIAAAERSGEKAWQAALRPRTFDDYI
                     GQRELIDNLKISVTAAKQNGWALDHFLFAGPPGLGKTTLAHVIANELGVNLHVSSGPA
                     IDHKGMLASLLTSLGEGDALFIDEIHRLAPVVEENLYPAMEDFRFDLFIGEGPHAKAV
                     TMQLPRFTLLGATTRMGLLSAPLLDRFGFHWQLRYYDLPDMKAIVKRSALLIGVPIDD
                     AGATEIARRSRGTPRIANRLLRRVRDFATVERDGAIDGDIASSSLDRLAVDHAGLDAL
                     DRGYLSVICERFDGGPVGIEAVAASLSEERGTLEEVVEPFLLQVGFIARTPRGRVATA
                     AGFAHMGLVAPSKAPSGGPGSAQGRLF"
     gene            1860..>2127
                     /locus_tag="VF516_20485"
     CDS             1860..>2127
                     /locus_tag="VF516_20485"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8110125.1"
                     /translation="MGIGKSSLSGGMLQLRRGGESSEVSRAGASTQPSVGKSTLVEQI
                     AGGGTRLPEQVRVKMERSLGADFSAVRIHEGPQAAAMGALAYTQG"
BASE COUNT          305 a          769 c          753 g          300 t
ORIGIN      
        1 cgcgacggcg agcacgtcgt gatcgactgc ggcggggtcg gctacgaggt cacgtgctcg
       61 gcgtacaccc tggcggcgct gccggcgcac ggcgagcgcg tcgcgctgcg cgtgttcacc
      121 caggtccgcg agacctagat cgcgctgttc ggcttcatcg acccccagga gcgcggcctg
      181 ttcgacctgc tgatcacggt caagaacgtc ggcccgagca ccgcgatcgc gatcctgtcc
      241 gggtcgagcc cgcgcgacat cgcgaccctg atcgcgcgcg aggacgtcgt cggcctcacc
      301 cggatcaagg gcatcggcaa gaagaccgcg gagctgctgg tcgtcgagct gcgcgacaag
      361 tgcgagctgc tgctgctgtc ctggagcgcg acggccgagg gcggcgtgcg gtcggtcgcg
      421 atcccggcgg gcgccgcgcg gccgcggtcc agcgtgcccc tgcgcagccc ggtcatcgcc
      481 gacgtcatga ccgcgctggt cgccatgggc tggcggccgg ccgaggccga ggccgcggtc
      541 agcgatctgc aggtcggcga caacgcgtcg ctggaatcgc tgctgcgcca ggcgctgcgt
      601 aatatgcccc ggtagcgcaa aggacccgcg tgccccgcaa gaaacacacc gacccgcccg
      661 gcgagccgcc cgacgccgcc cgcgagatcg ccgccgccga gcgctccggc gagaaggcct
      721 ggcaggccgc gctccggccg cgcacgttcg acgactacat cggccagcgc gagctgatcg
      781 acaacctcaa gatctccgtg accgccgcca agcagaacgg ctgggcgctc gatcacttcc
      841 tgttcgccgg cccgcccggc ctgggcaaga ccacgctcgc ccacgtcatc gccaacgagc
      901 tcggcgtcaa cctccacgtc tccagcggcc ctgcgatcga tcacaagggc atgctcgcgt
      961 cgctcttgac ctcgctcggc gagggcgacg cgctgttcat cgacgagatc caccggctcg
     1021 cgccggtcgt cgaggagaac ctctacccgg cgatggagga cttccggttc gacctgttca
     1081 tcggcgaggg cccgcacgcc aaggcggtga ccatgcagct gccgcggttc acgctgctcg
     1141 gcgcgaccac gcggatgggc cttctgtccg cgccgctgct cgaccgcttc gggttccact
     1201 ggcagctgcg ctactacgac cttcccgaca tgaaggcgat cgtcaagcgc tccgcgctgc
     1261 tgatcggcgt gccgatcgac gacgccggcg ccaccgagat cgcgcgccgg tcgcgcggca
     1321 cgccgcggat cgccaaccgc ctgctgcgcc gggtccgcga cttcgccacc gtggagcgcg
     1381 acggcgcgat cgacggcgac atcgcgtcgt cctcgctcga tcggctcgcg gtcgatcacg
     1441 ccgggctcga cgcgctcgac cgcggctacc tgtcggtgat ctgcgagcgg ttcgacgggg
     1501 gcccggtcgg catcgaggcg gtcgccgcgt cgctgtccga ggagcgcggc acgctcgagg
     1561 aggtcgtcga gccgtttctg ctccaggtcg gcttcatcgc gcgcacgccg cgcggccggg
     1621 tcgccaccgc ggcgggcttc gcgcacatgg gcctggtcgc gccgagcaag gcgccgtcgg
     1681 gcggccccgg cagcgcccag ggccggctgt tctgaccggc agatcaccgc cacaggtgct
     1741 cggaccaccg ccgccagggc accgaccggt gtgagatcac catgatgacg cagctcggtg
     1801 aagcgccacg gtaatggtgt gtaatcgacc gcggcatccg ggcggcttac ggttgcgaga
     1861 tgggcatcgg aaaaagctcg ttgtcggggg ggatgctgca gctccgccga ggaggagagt
     1921 ccagcgaggt atcgcgcgcc ggcgcctcga cgcagccgtc ggtcggcaag tcgacgctgg
     1981 tcgagcaaat cgccggcggc ggcacgcgct tgcccgagca agtgcgggtc aagatggagc
     2041 ggtcgcttgg tgctgacttc tcggccgtcc gcatccacga gggtccgcag gccgcggcga
     2101 tgggagcgct cgcctacacc cagggcg
//
LOCUS       DASVOR010001070         3549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_478447,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001070 DASVOR010000000
VERSION     DASVOR010001070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478447"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VF516_20490"
     CDS             <1..189
                     /locus_tag="VF516_20490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110126.1"
                     /translation="HRDECVRRMAQITDEIEGIDQLVRLIAQRTACPAFDPELDREID
                     RRLWELDEVDAAMHQLSA"
     gene            275..1555
                     /locus_tag="VF516_20495"
     CDS             275..1555
                     /locus_tag="VF516_20495"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110127.1"
                     /translation="MHDALPDVADLHGEIERLRAAVSHAERVNAVGRAIAGELDPLRL
                     AQTVADAATELTGAELGALFYSPGDGRTESYLLYAVSGMPRDRFARLALPRNTALLAP
                     AFLGQAAIRLSDVTADPRFARNYPHRGLPDGHPPVRSYLAVPVVSRTGLAIGSLLLGH
                     STPGVFTERAERTAVELAAYAATAMDNARLYGDAQRLIAQLEQANAELDQFTYVASHD
                     LRAPLRGISNLASWIDEDLPDTTAPEIKDQLRMLKVRAARMDRLIHGLLELARIGRTR
                     HQVERVDVTELLHETIELASPREASRIMMIGEMPMLTAERASLKQVFLHLLCNALQHA
                     GRDDVVIRISAVDRGDEWELAIEDNGVGIPREHHARVWQLFQTLQSRDAVDTTGIGLA
                     IVRKQVEGNGGRVWIDPDAASGTTIRFTWPKRPR"
     gene            1732..2994
                     /locus_tag="VF516_20500"
     CDS             1732..2994
                     /locus_tag="VF516_20500"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8110128.1"
                     /translation="MDRTEPPPYGASPSPTAHRIAALIRRHRDDILLRWEAAVRAMPR
                     ARELDPPTLIDHIPDLLERIAQAIEELSRGRAPTSHRVAAERHAMSRLDEGFDLQEVI
                     GELRLLRQGIFHVLDDPAAGIVQVDELRALDDAIDTTITASVEHYIQIRERTLQGFDR
                     VTTAALESSSLDDLLSRLLQVLHETTPAIDTSSIYLRDGDVLRVRAAVGVQGAVEQGA
                     AMRIGEGFAGAIAARNEPMTLRHPTAAQLRSPAMARVGVRVLHGVPIVDRGEVIGVAK
                     IGSLTAEQFSLQDQRIFAAIVQHMLRDQARRASEQLAEQERQFRALADNIPQLTWMAD
                     AAGERYWYNRRWYEYTGSTHEEMQGSGWQKLHHPDHAQRVYQRWMRAVEHGEPWEDIF
                     PLRGADGFYRWFLSRAVPIRDASGAIER"
     gene            3019..>3549
                     /locus_tag="VF516_20505"
     CDS             3019..>3549
                     /locus_tag="VF516_20505"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8110129.1"
                     /translation="MRRFLDNATEVLSRSLDERETLEQLARLAVPDIADWCVVDLVVQ
                     GQLEHVAIAHDDPDKLEAAWQYARTHRPDPAGYDASWQIMRTGKPGVTAEISDEMLAA
                     AARDPDHLRLMHELGFRSWIGAPLVARGTAIGVIHLCMNHSGRRYREPDLEIAVELGR
                     RAGVAIDNARLYHEAQT"
BASE COUNT          522 a         1323 c         1250 g          454 t
ORIGIN      
        1 caccgcgacg agtgcgtgcg ccggatggcg cagatcaccg acgagatcga gggcatcgac
       61 cagctggtcc ggttgatcgc tcagcgcacg gcgtgcccgg cgttcgaccc cgagctcgat
      121 cgcgagatcg accgccggct gtgggagctc gacgaggtcg acgccgcgat gcaccagctg
      181 tcggcgtagg gggtgcgcgc aagcgcacgg tctatatcct cgcgcaaatt tcacgctctc
      241 ccgcgcgccg acagtctaga gttgccgcgt cgtgatgcac gacgccctgc ccgacgtcgc
      301 ggacctgcac ggtgagatcg agcggctgcg cgccgcggtc agccacgccg agcgggtcaa
      361 cgcggtcggc cgcgcgatcg ccggcgagct cgacccgctg cggctcgccc agacggtggc
      421 ggacgcggcg accgagctca ccggcgccga gctcggcgcg ctgttctaca gccccggcga
      481 cgggcgcacg gagagctacc tgctgtacgc ggtgtccggg atgccgcgcg atcggttcgc
      541 ccggctcgcc ctgccgcgca acacggcgct gctggcgccg gcgttcctcg gccaggccgc
      601 gatccggctg tccgacgtca ccgccgatcc gcggttcgcc cggaactacc cgcaccgcgg
      661 gcttcccgac ggccatcctc cggtgcgcag ctacctcgcc gtgcccgtgg tgtcgcgcac
      721 cgggctcgcg atcggcagcc tgctgctcgg ccattccacg cccggggtgt tcaccgagcg
      781 cgccgagcgc accgcggtcg agctcgccgc gtacgccgcg accgcgatgg acaacgcgcg
      841 gctgtacggc gacgcccagc gcctgatcgc ccagctggag caggccaacg ccgagctcga
      901 ccagttcacc tacgtcgcgt cgcacgacct gcgcgcgccg ctgcgcggga tctccaacct
      961 cgcgtcctgg atcgacgagg atcttccgga caccaccgcc cccgagatca aggatcagct
     1021 ccgcatgctc aaggtccgcg ccgcgcgcat ggaccggctg atccacggcc tgctggagct
     1081 cgcgcggatc ggccgcaccc gccaccaggt cgagcgggtc gacgtcaccg agctgctgca
     1141 cgagaccatc gagctcgcca gcccgcgcga ggcgtcgcgg atcatgatga tcggcgagat
     1201 gccgatgctc accgcggagc gcgcgtcgct caagcaggtg tttctccacc tgctgtgcaa
     1261 cgcgctgcag cacgccggtc gcgacgacgt ggtgatccgg atctcggcgg tcgaccgcgg
     1321 cgacgagtgg gagctcgcga tcgaggacaa cggcgtcggg atcccgcgcg agcaccacgc
     1381 gcgggtctgg cagctgttcc agaccctgca gtcgcgcgac gcggtcgaca ccaccggcat
     1441 cgggctcgcg atcgtgcgca agcaggtcga gggcaacggc ggccgggtgt ggatcgaccc
     1501 cgacgcggcg tccggcacga cgatccgctt cacctggccc aagcggccgc ggtagaccgc
     1561 gcgctgcccg gcgcccggtc gtgccgggcc acgacagcgt ggcatccacg cacggtcgcg
     1621 ccgcggcccg atccggccgg aacgccgccc tccgccatgg ggttcgacta ccccgggcgt
     1681 ccacgcccgg gtcggaccac ttcatgcacc agcccagggg gacccagatc catggaccgg
     1741 accgagcctc ccccgtacgg cgcctcaccg agccccaccg cgcaccgcat cgccgcgctg
     1801 atccgccggc accgcgacga catcctcctc cgctgggagg cggcggtccg cgcgatgccg
     1861 cgggcgcgcg agctcgatcc gccgaccctg atcgatcaca tccccgatct gctggagcgc
     1921 atcgcgcagg cgatcgagga gctcagccgc ggccgggcgc ccacctcgca ccgggtggcg
     1981 gcggagcgcc acgcgatgtc ccggctggac gaggggttcg atctgcagga ggtgatcggc
     2041 gagctccggc tgctccgcca aggcatcttc cacgtgctcg acgatccggc ggcggggatc
     2101 gtccaggtcg acgagctgcg cgcgctcgac gacgcgatcg acacgacgat caccgcctcg
     2161 gtcgagcact acatccagat ccgcgagcgc acgctgcagg gcttcgaccg ggtcaccacc
     2221 gcggcgctcg agagcagcag cctggacgat ctgctgagcc ggctgctcca ggtgctgcac
     2281 gagaccacgc cggcgatcga caccagctcg atctacctgc gcgacggcga cgtgctgcgg
     2341 gtgcgcgccg cggtgggcgt gcagggcgcg gtcgagcaag gcgcggcgat gcggatcggc
     2401 gagggcttcg ccggcgcgat cgccgcgcgc aacgagccga tgacgctccg ccacccgacc
     2461 gcggcgcagc tgcgcagccc ggcgatggcg cgggtcgggg tccgcgtgct gcacggcgtg
     2521 ccgatcgtcg atcgcggcga ggtgatcggc gtggcgaaga tcggctcgct gaccgccgag
     2581 cagttctcgc tgcaggacca gcggatcttc gcggcgatcg tccagcacat gctgcgcgac
     2641 caggcgcggc gggcgtccga gcagctcgcc gagcaggagc gccagttccg cgcgctcgcc
     2701 gacaacatcc cgcagctgac ctggatggcc gacgccgccg gggagcgcta ctggtacaac
     2761 cggcgctggt acgagtacac cggcagcacg cacgaggaga tgcagggctc gggctggcag
     2821 aagctccacc accccgatca cgcccagcgc gtgtaccagc gctggatgcg cgcggtcgag
     2881 cacggcgaac cgtgggagga catcttcccg ctgcgcggcg ccgacgggtt ctaccgctgg
     2941 ttcctgtcgc gcgcggtgcc gatccgcgat gcatcgggcg cgatcgagcg ctagttcggc
     3001 accaacaccg acgtcaccgt gcggcggttc ctcgacaacg cgaccgaggt gctgagccgc
     3061 agcctggacg agcgcgagac gctcgagcag ctcgcccgcc tcgcggtgcc ggacatcgcg
     3121 gactggtgcg tcgtcgacct ggtggtgcag ggccagctgg agcacgtggc gatcgcgcac
     3181 gacgaccccg acaagctgga agcggcgtgg cagtacgcgc gcacccaccg gcccgacccc
     3241 gcgggctatg acgcctcgtg gcagatcatg cggaccggca agcccggcgt gacggcggag
     3301 atctccgacg agatgctcgc cgcggccgcg cgcgatcccg accacctgcg cctgatgcac
     3361 gagctcggct tccggtcgtg gatcggcgcc ccgctggtcg cgcgggggac cgcgatcggc
     3421 gtgatccacc tgtgcatgaa ccactccggc cgccgctacc gcgagcccga tctggagatc
     3481 gcggtcgagc tcgggcgccg cgccggggtc gcgatcgaca acgcccggct gtaccacgag
     3541 gcccagacc
//
LOCUS       DASVOR010001071         2847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_332128,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001071 DASVOR010000000
VERSION     DASVOR010001071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332128"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1816)
                     /locus_tag="VF516_20510"
     CDS             complement(<1..1816)
                     /locus_tag="VF516_20510"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HEX8110130.1"
                     /translation="MRPVLLAACALIACSHPPGPSAPPLGDGSWKPVGHEGRPPAPGR
                     AVTISIVGTNDLHGALERLPLFAGFVANLRVARAADGGGVVLVDGGDMFQGTLESNLG
                     EGADVVRAYNYLGYAGTAIGNHEFDYGPEGPAATPTSPTDDPRGALKARAREARFPFL
                     ASNIVDNATGSRILWPNMPGSTMVEVAGVKIGIIGASTEATPHTTMPANFAGLRISPP
                     AQAIVDHATALRGQGARVIIATMHIGTNCKQFDHPDDSSSCDRREELFRLLGDLPHGT
                     VDVIIGGHTHAGVAHFLAGVPVIESYSSGRAFGRVDLTVGPDGKITDLRIDKPHLMCP
                     VDKNFNPVPVADCHPGDYEGKPVAPDPEVQKIVDAALARAGERRQEKLGVVLASIVKR
                     AWSVESPEGNLFTDLMLEARPDAQVALTNGGGLRADLPAGTLTYGALFEAMPFDNRFA
                     IVELTGKDLRTLVTSNLSRSGGILSWAGLRARARCKAGKLDLQIQVQGKPLDENASYK
                     LATSDFLASGGDGLIGRLQLSEGKIQVTSTIIREAVADVLRGWKASPRAAIDPGRLYS
                     ATRPRLDYEGKRPVACGEPWANEMSQYSGWWRSANPPST"
     gene            2039..>2847
                     /locus_tag="VF516_20515"
     CDS             2039..>2847
                     /locus_tag="VF516_20515"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110131.1"
                     /translation="MRSQLHRLVVSTGLAAATLLGAAGSSFAQPRDHRHNPHDVDAAP
                     REAPPPPRGEPHDARKVGFVWIGGRWDWNRRERRWDWLPGRWEREHRGKRWREARWEQ
                     RDGVYVLIDGDWIEAERRPSQPPPPPHEERLERRPGFVWLAGRWDWRDGNWVWIDGRF
                     ERERRGKRWREARWEQRDGVYVLVDGDWIDAAASRFPVEAPPAPRPEPHDPRKVGFVW
                     IGGRWDWQNGSWTWLPGHWERERVRMRWLDGRWELRGDHWEWIDGSWVEETV"
BASE COUNT          379 a         1021 c         1046 g          401 t
ORIGIN      
        1 tggtcgacgg cgggttcgcc gacctccacc accccgagta ctgggacatc tcgttcgccc
       61 acggctcgcc gcacgcgacc gggcgcttgc cctcgtagtc gagccgcggc cgggtcgccg
      121 agtacagccg ccccgggtcg atcgcggcgc gcggcgacgc cttccagccg cgcagcacgt
      181 cggccaccgc ctcgcggatg atcgtgctgg tcacctggat cttgccctcg gacagctgga
      241 gccggccgat caagccgtct ccgcccgagg ccagaaagtc gctggtcgcc agcttgtagc
      301 tcgcgttctc gtcgagcggc ttgccctgga cctggatctg caagtcgagc ttgccggcct
      361 tgcagcgcgc cctggctctc agtccggccc acgacaggat gccgccgctg cgcgacaggt
      421 tcgaggtcac cagcgtgcgc aggtccttgc cggtgagctc gacgatcgcg aaccggttgt
      481 cgaacggcat cgcctcgaac agcgcgccgt aggtcagggt gccggccggc aggtcggcgc
      541 gcagcccgcc gccgttggtc agcgcgacct gggcgtcggg gcgggcctcg agcatcagat
      601 cggtgaacag gttgccctcg ggcgactcga ccgaccacgc tcgcttcacg atgctggcga
      661 gcacgacgcc gagcttctcc tggcggcgct cgccggcgcg cgccagcgcc gcatcgacga
      721 tcttctggac ctcgggatcg ggggccaccg gcttgccctc gtagtcaccc gggtggcagt
      781 ccgcgaccgg caccgggttg aagttcttgt cgaccgggca catcaggtgc ggcttgtcga
      841 tgcggaggtc ggtgatcttg ccgtcggggc cgaccgtcag atcgacccgg ccgaacgcgc
      901 ggccgctgga gtacgactcg atcacgggga cgccggcgag gaagtgcgcg accccggcgt
      961 gggtgtggcc gccgatgatg acgtcgaccg tgccgtgcgg caggtcgccg agcaggcgga
     1021 acagctcctc gcgcctgtcg cacgacgagc tgtcgtcggg gtgatcgaac tgcttgcagt
     1081 tcgtcccgat gtgcatggtc gcgatgatca cgcgcgcgcc ctggccgcgc agcgcggtcg
     1141 cgtgatcgac gatcgcctgc gccggcggcg agatccgcag cccggcgaag ttcgccggca
     1201 tcgtggtgtg cggcgtcgcc tcggtgctcg cgccgatgat cccgatcttg accccggcga
     1261 cctcgaccat cgtcgagccc ggcatgttgg gccacaggat ccgcgagccg gtcgcgttgt
     1321 cgacgatgtt cgacgccagg aacggaaacc tggcctcgcg cgcccgggcc ttgagcgcgc
     1381 cgcgcgggtc gtcggtcggg ctggtcgggg tcgccgccgg gccctcgggg ccgtagtcga
     1441 actcgtggtt gccgatcgcg gtcccggcgt agccgaggta gttgtaggcc cgcaccacgt
     1501 cggcgccctc gccgaggttg gactccagcg tgccctggaa catgtcgccg ccgtcgacca
     1561 gcaccacgcc gccgccgtcg gccgcgcgcg ccacgcgcag gttggcgacg aacccggcga
     1621 acagcggcag gcgctccagc gcgccgtgca ggtcgttggt cccgacgatc gagatcgtca
     1681 ccgcgcggcc gggcgccggc ggccgcccct cgtgtccgac cggcttccag ctgccgtcgc
     1741 cgagcggcgg tgcactcgga ccgggcggat gcgagcacgc gatcagggcg caggcggcga
     1801 gcaggaccgg tctcatgctg cgcggactct acccccggat cctcgcaggg cgaagatcga
     1861 acgtcgtcca cgcgcgcagg tgatccgacg cgtcggtggc gcggcggagg ttcaggcagg
     1921 gcaaccacat gcaccgcgca agccggcgta acctggccgt caccgacgct gcccgcgcgc
     1981 caccgcgcgt gaagattcga ggatagactc cgtcaggttc aggaggtcag gaggatcgat
     2041 gcgttctcaa ctacatcgac tggtcgtctc gacggggctc gccgccgcga cgctgctcgg
     2101 cgcagccggg agctcgttcg cgcagccgcg cgatcaccgt cacaatccgc acgacgtcga
     2161 cgccgcgccc cgcgaggcgc cgccgccgcc gcgcggggag ccccacgacg cacgcaaggt
     2221 cggcttcgtc tggatcggcg ggcgctggga ctggaaccgc cgcgagcgcc gctgggactg
     2281 gctccccggg cgctgggagc gcgagcaccg cggcaagcgc tggcgcgagg cgcgctggga
     2341 gcagcgcgac ggcgtctacg tcctgatcga cggcgactgg atcgaggccg agcgccgtcc
     2401 gagccagccg ccgccaccac cgcacgagga gcgcctcgag cgccgccccg gcttcgtctg
     2461 gctcgccggc cgctgggact ggcgcgacgg caactgggtc tggatcgacg gccggttcga
     2521 gcgcgagcgc cgcggcaagc gctggcgcga ggcgcgctgg gagcagcgcg acggcgtcta
     2581 cgtcctggtc gacggcgact ggatcgacgc cgcggcctcg cggtttcccg tcgaggcgcc
     2641 gcctgccccg cgccccgagc cccacgaccc gcgcaaggtc ggcttcgtct ggatcggcgg
     2701 gcgctgggac tggcagaacg gcagctggac ctggctgccc ggccactggg agcgcgagcg
     2761 cgtccggatg cgctggctcg acggccgctg ggagcttcgc ggcgatcact gggaatggat
     2821 cgacggcagc tgggtcgagg agaccgt
//
LOCUS       DASVOR010001072         4685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390427,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001072 DASVOR010000000
VERSION     DASVOR010001072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390427"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            121..2652
                     /locus_tag="VF516_20520"
     CDS             121..2652
                     /locus_tag="VF516_20520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110132.1"
                     /translation="MLTSGGDVIAEVPAGFFAEMAREGRGRLRDGRLLTGDGWRSPAG
                     RDYAAVLTVHLRRYPEGHRNRDRQRVSGITVVGGEVKTVRAYAEVPAARIGLGYGVLR
                     GIPLEPFKTLAADLGGFESSDERYRGAWSEGKQRYLVVDGKTGLVPTGTRVYVRQLDG
                     VMLPNGKRHDGWLVVNDTRAGTYGAHFDVFVGDRQYGKELVALTGGPFVDVWFDGIEA
                     RVPAGYDHGFPRGKSALADIAIEATPHAVEVEIQINNTSAATDDMVRVRSTAPAERPT
                     VACRIRLVGTAATAQSVVLTNPDGRLRFPAAADTTKAVTLPANGDWVAFDLSGETVSA
                     ALGDARIEVHSSSATGALIGTKDLTVFEVQVEINHTPAVDDDIVPLHTTTPPHRPVIR
                     SRARIAPAAPAAISVLLTNPDGRLRFPSATDTTLALRLAAGGAWSNFEISGETISGAV
                     GDAQIEVHRTNAAGPILGRRSVTVIAIQVEINNTPAVNDDIVQLHCTHPAQRPVVHSR
                     LRIAPAAPAAIPVLLTNPDGRLRFPDPAHTTLAVTLAANGAWSTFDISGQTASAAIGD
                     AHIQVHRTNASGVILGRRDVTVFHFDSAHMDLTQGGNYTINGGNFTVAAGVAVNFSAS
                     AALKPAGLDCAAPQIANLRIGIMQESRNFIITTTWDTPTIAWAPGVAPGFAFNVPAVM
                     RETITYAATVAQPVNDGLAGAYPLYSQQAGALMPPTGCTGSGAATSSDTPGQGVAPTF
                     SQTFANAAGPIGVVTWNNVIRVTRTENFRTFCVVFNTVTNDFCNLRQATWALNANSNS
                     ANQHATVSGDAAPTVNPATGVQANNAANTTVHAAVAAATHHFVHP"
     gene            2784..3527
                     /locus_tag="VF516_20525"
     CDS             2784..3527
                     /locus_tag="VF516_20525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110133.1"
                     /translation="MTIGASVDVVSGKLIDDVTRREIAPEEARSGIIFAGTWRLVDTS
                     GEEAIPRSASYSWIEPPRTHLRRLTAEDLYDPFARGGTILCRYHAEGQGRAQFSSVRV
                     LPPGWESSVRVARAALRADPELLAGPRLLSVVGGDNPVLAVLAFRKLTDPRSGLLRAA
                     GYTQAAFVYLSMHRTEDAVLQELMRLGPSLADPRFFVLGVCAARLVDPELRSSQQWAA
                     ALVASLRAPTDPHVQQLLQAARLEPILQA"
     gene            3524..4387
                     /locus_tag="VF516_20530"
     CDS             3524..4387
                     /locus_tag="VF516_20530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110134.1"
                     /translation="MRWHAVVVIAGCLVASAHADPVAPLELAARTHGSALELEFRNRG
                     STPVALPINTAGYDDRLSITLTRPGAPRLDYRFREPRDRRVARSTTIAPHATYTETFD
                     LVSSALRGNVDPPPPGNYQVSAVWTHPGGSPLTATTELAIAAPVEQPCKSRPVASGIE
                     LLARQVAAAGTVEIGLHNTSSASICVAARVDAAFPQSDWLTVELEDTHGKPLRTLRFV
                     AVRHASVRVTVELAPGATTWGRWDLAEWARRSSSAPLHGLMRATITYHGERETDVFHG
                     KLVASLAVNVR"
     gene            complement(4423..>4685)
                     /locus_tag="VF516_20535"
     CDS             complement(4423..>4685)
                     /locus_tag="VF516_20535"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEX8110135.1"
                     /translation="HRPPPDPPRTTTDELLRAYQTLGVSPTATDEEVQQAWRRLRVEL
                     HPDAAAGDPVEFERRSRLSAELNRARDIIFAHRRRTAGRRSA"
BASE COUNT          705 a         1656 c         1615 g          709 t
ORIGIN      
        1 gtcacgcccg agcgcggcgg ggcggcgccg tcggtgcccg cgaccgcgtt ccggcgctgg
       61 tacctcaccc cctaccatgt gcccgtcgag cccgacgcgt cccgcgatcc gctcgtcccg
      121 gtgctgacca gcggcggcga cgtgatcgcc gaggtgccgg ccggcttctt cgccgagatg
      181 gcgcgggagg ggcgggggcg cctgcgcgac ggccgcctgc tgaccggcga cggatggcgc
      241 tcgcccgccg gccgcgacta cgcagcggtc ctgaccgtgc atctgcggcg ttatcccgag
      301 ggacaccgga accgcgaccg gcagcgggtc tcgggcatca cggtggttgg cggcgaggtg
      361 aagacggtac gggcgtacgc cgaggtgccg gcggccagga tcggcctggg ctacggcgtg
      421 ctgcgcggca tcccgctcga gccgttcaag acgctggccg ccgacctcgg cggcttcgag
      481 agctccgacg agcgctatcg cggcgcatgg agcgagggca agcagcgcta cctcgtggtc
      541 gacggcaaga ccgggctggt tcccaccggg acgcgggtct acgtgcgcca gctcgacggc
      601 gtcatgctgc cgaacggcaa gcggcacgac ggctggctgg tcgtcaacga cacccgcgct
      661 ggcacctacg gcgcgcactt cgacgtgttc gtgggtgaca ggcagtacgg caaggagctg
      721 gtggcgctga ccggggggcc tttcgtcgat gtctggttcg atggcatcga ggcgcgcgtt
      781 cccgccggct acgaccacgg cttccccagg ggcaagtccg cgctcgccga catcgcgatc
      841 gaggcaacgc cgcacgccgt cgaggtggag atccagatca acaacacatc cgcggcgacc
      901 gacgatatgg tgcgcgtcag gtcgaccgcg cccgccgagc gcccgacggt ggcttgccgc
      961 atccgcctgg tcgggacggc ggccaccgcg cagagcgtcg tgctgaccaa tcccgacggc
     1021 cgcctgcggt tccccgcagc ggcggatacc acgaaggccg tcacgcttcc cgcgaacggc
     1081 gactgggtcg cgttcgacct ctcgggcgag acggtcagcg cggcgctcgg agacgccagg
     1141 atcgaggtcc actcgagctc cgcgaccgga gcactgatcg gcaccaagga cctgacggtc
     1201 ttcgaggtcc aggtcgagat caaccatacc ccggcggtcg atgacgacat cgtgccgctt
     1261 cacaccacga cgcctccgca ccgcccggtc atccgttccc gcgcgcggat cgccccggcg
     1321 gcacccgcgg ccatctccgt gctgctcacg aacccggacg gccggttgcg atttccgagc
     1381 gcgaccgata cgacgctggc gctgaggctc gccgccggcg gtgcatggtc gaacttcgag
     1441 atctcgggtg agacgatcag cggggccgtc ggcgatgccc agatcgaggt ccaccggacg
     1501 aacgcggccg ggccgattct cggtcgaagg agcgtgaccg tcatcgcgat ccaggtcgag
     1561 atcaacaata ctccagcggt caacgacgac atcgtgcagc ttcattgcac gcatcctgca
     1621 cagcgcccgg tcgtccattc ccgcctgcgg atcgcccccg cggcaccggc tgccattccg
     1681 gtgctgctca cgaatccgga cggccggctg cgcttcccag atccggccca taccacgctg
     1741 gccgtcacgc tcgccgcgaa cggtgcgtgg tccacgttcg acatttcggg gcaaaccgcg
     1801 agcgcggcga tcggggatgc gcacatccag gtccatcgga cgaacgcaag cggcgtgatc
     1861 ctgggccgca gagacgtgac ggtgttccac ttcgattctg cccatatgga tctcacccag
     1921 ggcggtaact acacgatcaa cggcggcaac ttcaccgtgg cggccggcgt cgccgtgaat
     1981 ttttcggcct cggcggccct caagccggcc gggctggatt gcgcagcgcc tcagattgcc
     2041 aatctgcgga tcggcatcat gcaagagagc cgtaacttca tcatcaccac cacctgggac
     2101 acgccgacga tcgcgtgggc gcccggggtg gcgccggggt tcgcgttcaa tgtcccggcc
     2161 gtgatgcgcg agaccatcac ctacgcggcc acggtggcgc agccggtcaa cgacggtctc
     2221 gcaggtgcct acccgctcta ctcgcagcag gcgggtgcgc tgatgccacc gaccggctgt
     2281 accggcagtg gtgcagccac cagcagcgat acgcctggac agggcgtggc gcccacgttc
     2341 agccagacgt tcgcgaacgc tgcggggccg atcggcgtcg tgacctggaa caacgtgatc
     2401 cgggttactc gcaccgagaa ctttcgcacc ttctgcgtgg tattcaatac cgtcaccaat
     2461 gacttctgca acctgcgcca ggcgacctgg gccttgaacg cgaacagcaa cagcgcgaac
     2521 cagcacgcga ccgtcagcgg cgacgccgcg ccgaccgtca atccggcgac cggcgtacag
     2581 gcaaacaacg cagccaacac caccgtccat gcggccgtgg cggctgccac gcatcacttc
     2641 gtgcatccat gacagccaga ccggcgtccc catttcacga tatacgggcc gtgcacgaca
     2701 tggcgagcct cggcgtcgac gacgtggccg cggcgccgtg gatcctgatc gagatcaccg
     2761 cgcagccctg gagcggtggc gcgatgacga tcggcgcctc ggtcgatgtc gtgtcgggca
     2821 agctgatcga cgacgtcacc cggcgcgaga tcgcgcccga ggaggcccgg agcggcatca
     2881 tctttgcggg cacctggagg ctcgtggaca cctcggggga ggaggcgatc ccccgcagcg
     2941 ccagctattc atggatcgag ccaccgagga cgcatctgcg caggttgacg gcggaagatc
     3001 tgtacgaccc gttcgcgcgt ggcgggacga tcctgtgccg gtatcacgcc gaggggcaag
     3061 gtcgagcgca gttctcctcg gtccgcgtcc tgccgccggg ctgggagagc agcgtgcggg
     3121 tcgcgcgcgc ggcgttgcgc gcggatcccg agctgctcgc cggacccagg ttgctgtccg
     3181 tggtcggggg tgacaatccg gtcctggcgg tcctggcgtt tcgcaagctc accgatccgc
     3241 gcagcgggct cctccgtgcc gcaggatata cccaggcggc cttcgtctac ctgagcatgc
     3301 accgcaccga ggacgctgtc cttcaggagc tgatgaggct cggcccgtcg ctcgcggacc
     3361 cgcggttctt cgtgctgggg gtgtgcgccg cccggctggt ggaccccgag ctgcgctcgt
     3421 cgcagcagtg ggctgcggcg ctcgtcgcct cgctgcgagc gcccaccgac ccccatgtgc
     3481 agcaactgtt gcaggctgcg cgcctcgagc caatactcca ggcatgaggt ggcacgccgt
     3541 cgtcgtgatc gcgggatgcc tggtcgcgtc ggcccacgcc gatccggtcg cgccgctcga
     3601 gctcgccgcc cgcacccacg gcagtgctct ggagctcgag ttccgtaacc ggggatcgac
     3661 ccccgtcgcg ctgccgatca acaccgctgg ctacgacgac cggttgtcga tcaccttgac
     3721 tcgaccgggc gcaccgcggc tcgactaccg ctttcgcgag ccccgggaca ggcgtgtcgc
     3781 acgatcgacc acgattgcgc cgcacgcgac ctacaccgag accttcgacc tggtgagcag
     3841 tgcgctgcgc ggcaacgtcg atcctccgcc gccggggaac taccaggtca gcgccgtctg
     3901 gacccacccg ggcggatcgc cgctcaccgc cacgacggag ctcgcgatcg ccgcgccggt
     3961 cgagcagccc tgcaagtcgc ggcccgtggc cagcggcatc gagctgctcg cgcgccaggt
     4021 ggcggcggcc gggaccgtcg agatcggcct ccacaacacg tcgagcgcgt cgatctgcgt
     4081 cgcggcgcgg gtcgacgccg cgtttccgca gagtgactgg ttgacggtcg agctcgagga
     4141 tacgcacggc aagccgctgc gtaccctgcg gttcgtcgct gttcgtcatg cctcggtgcg
     4201 agtcaccgtc gagctcgcgc ccggggccac cacgtggggg cgctgggacc tcgccgagtg
     4261 ggccaggcgc tcgtcatcgg ctccgctgca cggcctgatg cgcgcgacga tcacctatca
     4321 tggcgagcga gagaccgatg tgtttcacgg caagctcgtc gcgagcctcg ccgtcaacgt
     4381 gcgctgagcg gatcgcatcc tgctcggtcg tgtcgcaaag ctctacgcgc ttcgccggcc
     4441 ggcggtgcgc cggcgatgcg cgaagatgat atcgcgcgcg cggttgagct cggccgagag
     4501 gcggctccgc cgctcgaact ccaccgggtc gccggccgcg gcgtcggggt gtagctccac
     4561 gcggaggcgg cgccatgcct gttgcacctc ttcgtccgtg gcggtcggcg acacgcccag
     4621 cgtctggtag gcgcgcaaga gctcgtcggt cgtggtccgc ggcgggtcgg gcggtgggcg
     4681 gtgcg
//
LOCUS       DASVOR010001073         5585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_536890,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001073 DASVOR010000000
VERSION     DASVOR010001073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536890"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..421)
                     /locus_tag="VF516_20540"
     CDS             complement(<1..421)
                     /locus_tag="VF516_20540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110136.1"
                     /translation="MDRSSGITCVLLTLATASGSALAHPAAGAQVCTLGAAAVGQSGA
                     VVCKDVRTGATTQQIAVGNTVVGSGGTGGTLSRHRDDVLVTNLAGGAVLFEEVAGRLV
                     HPVRLQTDGESSLSGALGERGAYVVTPTRVLFFPRGRS"
     gene            889..1197
                     /locus_tag="VF516_20545"
     CDS             889..1197
                     /locus_tag="VF516_20545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8110137.1"
                     /translation="MALPVIVLDLGGKLLALNSAAMRLGSRQRHEVVGRLASEYAPGI
                     EYLWDERVAAGRAPGGATFEIAIKTQRGALLLEYVMKVYELDGQPAVVAVVTSARPLT
                     "
     gene            1287..1901
                     /gene="msrA"
                     /locus_tag="VF516_20550"
     CDS             1287..1901
                     /gene="msrA"
                     /locus_tag="VF516_20550"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEX8110138.1"
                     /translation="MASGSLRALLLLAVLSIGCEPTSSAPASIARPAIDPRGPREIAI
                     VAGGCFWGMEKVMRQAPGVVSVEVGYAGGKSAHVTYEEVSEGDTGHAEAVRIVFDPNQ
                     VSYEQLLTHWFFRGHDPTTLDRQNNDVGTQYRSAIFPTSEAQRKTAQAVKARVDRSGK
                     WGRPIVTRIEPAATWVRAEESHQDYLIKHPDGYDDHWIRAFDFD"
     gene            complement(1915..2853)
                     /gene="cysK"
                     /locus_tag="VF516_20555"
     CDS             complement(1915..2853)
                     /gene="cysK"
                     /locus_tag="VF516_20555"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:TIGR01136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEX8110139.1"
                     /translation="MIYSSILDTIGRTPIVRINRLAPPAVTMYVKVESFNPGGSVKDR
                     LAIGIIEDAEQRGLLEPGQTVVEATSGNTGIALAMVCAAKGYPFVAVMVETFSVERRQ
                     IMRAFGAKVVLTPAAERGSGMVRKAAELAEQHGWFLARQFENPANPAYHRQTTGPEIL
                     SDFAGKRLDMFVTGWGTGGTLTGAGQVLKRARPDLKIVVCEPAEAQLLGDQAFKSHKI
                     QGWTPDFVPAVLDRTVFDERLAIEDGEAIATARELAHKEGIFVGISAGGTFAGALRVA
                     RAAPAGSVLLAMLPDTGERYLSTALFEGVNQGSDPV"
     gene            2942..3952
                     /locus_tag="VF516_20560"
     CDS             2942..3952
                     /locus_tag="VF516_20560"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8110140.1"
                     /translation="MPVIRHRLTSRVLAGNPLGDPIERDLFVYVPPGYDPAATRRWPA
                     VLALVGFTGTGGGLFNVDALAEPLHAKLDRLIAAGACPPMIVAAPDCFTRVGGNQYIN
                     SAGTGRYEDYLLDEILPFVDASYRTLPRGQWGVLGKSSGGYGSIVLGMRHPEVFCALA
                     DHSGDSNFELCYIPDFPGALDAFREAGGPAAWLDRYWADVNRRRAKHFKPLNIVGMAA
                     HYSPDPDAPAAHLRIDWPFDLDTGEFRPAVWDRWRAWDPIQMVDAHADRLRRLRAIYI
                     DCGTKDEFALHWGARTLAARIAGHGIAVRHEEFDDGHMNITYRYDVSLPFLGEAILAA
                     GG"
     gene            complement(3968..4300)
                     /locus_tag="VF516_20565"
     CDS             complement(3968..4300)
                     /locus_tag="VF516_20565"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8110141.1"
                     /translation="MVQAVTAAGLAELTSAQIVDLVDVRDPDEWQAGHIAGARLVPLE
                     RLRADPDAALARDVATVFICARGVRSLQAAKLAERFGYQRVYHLEGGVKEWARSGKAL
                     VAASRLAA"
     gene            complement(4345..>5585)
                     /locus_tag="VF516_20570"
     CDS             complement(4345..>5585)
                     /locus_tag="VF516_20570"
                     /inference="COORDINATES: protein motif:HMM:NF041166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0140737 - encapsulin nanocompartment
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="family 2A encapsulin nanocompartment cargo
                     protein cysteine desulfurase"
                     /protein_id="HEX8110142.1"
                     /translation="RDLGGTGFDPYALRRDFPILEERVHGGKRLVWLDNAATTQKPHA
                     VIDRLTYFYEHENSNVHRAAHVLAARATDAYESARDTTRKFLNAPSASEIVFVRGATE
                     GINLIAKAWGARHVERGDEIVVTHLEHHANIVPWQQLCAAAGARLRVAPVDERGDVIL
                     EAYEKLLCSRTRLVAFTQVSNALGTVTPAREMIEIAHRWGATVVLDGAQAVSHMPVDV
                     QALDCDFYVFSGHKVFAPTGIGAVFGKRALLDAMPPWQGGGNMIADVTFERTLYQQPP
                     WRFEAGTGNIADAAGLGAALDYVLRIGMPTIERYEHELLGYATERLATVPGLRMVGTP
                     RHKAGVLSFVLDGQRTEDIGAALDKDGIAVRSGHHCAQPILRRYGLESTVRASLAFYN
                     VHEDIDALVASLKRIQAASA"
BASE COUNT          793 a         2008 c         1964 g          820 t
ORIGIN      
        1 cgctgcggcc gcgcgggaag aacagcacgc gggtcggcgt caccacgtac gcgccgcgct
       61 cgccgagcgc gccggacagc gagctctcgc cgtcggtctg gagccggacc gggtggacga
      121 gccggccggc gacctcctcg aacaggaccg cgccgcccgc gaggttggtg acgagcacgt
      181 cgtcgcggtg gcggctgagc gtgccgccgg tgccaccgga gccgacgacg gtgttgccga
      241 ccgcgatctg ctgggtggtc gcgccggtgc gcacatcctt gcagacgacg gcgccggact
      301 ggccgaccgc cgccgcgccg agcgtgcaga cctgggcccc ggcggcgggg tgggccagcg
      361 cgctgcccga ggcggtggcg agggtgagca gaacacaggt gattccagac gagcgatcca
      421 tgattcctcc cgaccaacgt gaaccgtccg cgccgggagc atcccggcgc gatacgcgtg
      481 gccgcatgcc cggcgcgcga cggcgtcgtc gcgaggtgca cgggcgatgc ggtgtgtcgc
      541 gactacggcg ttcgccggga tccggttaca ggcgcggcgc cggatcgccg ggcgatcatg
      601 tcgatcctcc gcgacccggg ccgggtgaca ctgcgcgcac gggcacagtc aggcggtggc
      661 aggcagaaca ggcgccgtgt tccggccacg gtcgcatccc ggtcgcggtc gagccggcgg
      721 tccggagcca ctacgtgagc tgtttctcca tggcccgatg gcagatcgcg ggcagtcgcc
      781 ctcgacgtct cccgtatgat gcgagatctc ggcgccccgg accgagcttc ctttcctgac
      841 tccgtgactg ctgtaacgtt tcctacctcc ctggccgatc tggaagtgat ggccttgccg
      901 gtgatcgtgc tcgatctcgg tgggaagctc cttgcactga actcggcggc gatgcggctc
      961 ggatcccggc agcggcacga ggtcgtcggc aggctggcgt cggagtacgc gcccgggatc
     1021 gagtacctgt gggacgagcg ggtcgctgcg gggcgcgcgc ccgggggcgc gacgttcgag
     1081 atcgcgatca agacccagcg cggcgcgctc ctgctggagt acgtcatgaa ggtctacgag
     1141 ctcgatggcc agcccgccgt cgtcgcggtg gtcaccagcg cccgaccgct gacctgactc
     1201 cgggagtggc tccggcggac gcggacttgc cgccctgcgc cgtaacgcgc gcgcgcggca
     1261 ggcgtagctg gggcatgatg aggtccatgg cgagcgggtc gctccgggcg ctgttgctgc
     1321 tggccgtgct gagcatcggc tgcgagccca ccagctcggc ccccgcgtcg atcgcccggc
     1381 ccgcgatcga tccccgcggt ccgcgcgaga tcgcgatcgt cgccggcggc tgcttctggg
     1441 gcatggagaa ggtcatgcgc caggcgcccg gcgtggtgtc ggtcgaggtc gggtacgcgg
     1501 gaggcaagtc ggcccatgtc acctacgagg aggtgtccga gggcgacacc ggccacgccg
     1561 aggcggtgcg gatcgtgttc gatcccaacc aggtctcgta cgagcagtta ttgacccact
     1621 ggttcttccg cggccacgac ccgacgacgc tcgatcgcca gaacaacgac gtcggcacgc
     1681 agtaccgctc ggcgatcttt ccgacgtcgg aggcgcagcg caagaccgcg caggcggtca
     1741 aggcgcgggt cgaccggtcg ggcaagtggg gccggccgat cgtcacccgg atcgagccgg
     1801 cggccacctg ggtgcgcgcc gaggaatctc accaggacta cctgatcaag caccccgacg
     1861 gctacgacga ccactggatc cgcgcgttcg acttcgattg atctgcccct cgggctacac
     1921 cggatccgag ccctggttca cgccctcgaa cagcgcggtc gacaggtagc gctcgccggt
     1981 gtcgggcagc atggcgagca ggacgctgcc ggccggcgcc gcgcgcgcca cccgcagcgc
     2041 gcccgcgaac gtgccgcctg ccgagatccc gacgaagatg ccctccttgt gggcgagctc
     2101 gcgcgcggtc gcgatcgcct cgccgtcctc gatcgccagc cgctcgtcga acaccgtccg
     2161 gtcgagcacc gcgggcacga agtcgggcgt ccagccctgg atcttgtgcg acttgaacgc
     2221 ctggtcgccc agcagctggg cctcggcggg ctcgcacacc acgatcttga gatcgggccg
     2281 ggcccgcttg agcacctgac cggccccggt cagcgtgccg ccggtgcccc acccggtgac
     2341 gaacatgtcg agccgcttgc cggcgaagtc ggacaggatc tccggccccg tggtctggcg
     2401 gtggtacgcc gggttcgccg ggttctcgaa ctggcgggcg aggaaccagc cgtgctgctc
     2461 ggcgagctcg gcggccttgc gcaccatccc gctgccgcgc tccgcggccg gcgtcagcac
     2521 gaccttggcg ccgaacgcgc gcatgatctg gcggcgctcg accgagaacg tctccaccat
     2581 cacggcgacg aacgggtagc ccttggcggc gcacaccatc gccagcgcga tgccggtgtt
     2641 gcccgacgtc gcctcgacga cggtctggcc cggctcgagc aggccgcgct gctcggcgtc
     2701 ctcgatgatg ccgatcgcga gccggtcctt gaccgagccg ccggggttga acgactcgac
     2761 cttgacgtac atcgtcaccg cgggcggggc gagccggttg atgcgaacga tcggtgtgcg
     2821 cccgatcgtg tccaaaatgg aggaatagat catggcaatg agctcaccat gattccgcgg
     2881 cgcagccaat cgcaggttgt gcgccggtcg cgcaccgcgc gatctgtgct gtagtcgcgc
     2941 catgcccgtg atccgccacc gcctgaccag ccgcgtgctc gccggtaacc cgctgggcga
     3001 tccgatcgag cgcgacctgt tcgtctacgt gccgcccggc tacgaccccg ccgcgacccg
     3061 gcgctggccc gccgtgctcg ccctcgtcgg cttcaccggg accggcggcg ggctgttcaa
     3121 tgtcgatgcg ctggccgagc cgctgcacgc caagctcgac cgcttgatcg ccgccggcgc
     3181 atgcccgccg atgatcgtcg ccgcgcccga ctgcttcacc cgggtcggcg gcaaccagta
     3241 catcaacagc gccgggaccg gccgctacga ggattacttg ctcgacgaga tcttgccatt
     3301 cgtggacgcc agctaccgca cactgccgcg cggccagtgg ggcgtattgg gcaagtcgtc
     3361 cggcggctac ggctcgatcg tgctcggcat gcgccacccc gaggtgttct gcgcgctcgc
     3421 cgatcactcc ggcgacagca acttcgaact ctgttacatc cccgacttcc cgggcgcgct
     3481 cgacgcgttt cgcgaggccg gcggcccggc ggcctggctc gatcgctact gggccgacgt
     3541 caaccgccgc cgcgcgaagc atttcaagcc gctcaacatc gtcggcatgg ctgcccacta
     3601 ctcgcccgac cccgatgccc cggccgccca cctgcggatc gactggccgt tcgatctcga
     3661 caccggcgag tttcgccccg cggtgtggga tcgctggcgg gcctgggacc cgatccagat
     3721 ggtcgatgcg cacgccgacc ggctacgccg gctgcgcgcg atctacatcg actgcggaac
     3781 gaaggacgag ttcgcgctgc actggggcgc ccgcacgctg gccgcccgga tcgccggcca
     3841 cggcatcgcc gtgcgccacg aggagttcga cgacggccac atgaacatca cctaccgcta
     3901 cgacgtcagc ctgccgttcc tcggcgaggc gatcctggcg gccggcgggt agcggcccgc
     3961 gcgcagctca cgcggcgagg cgcgacgcgg ccacgagcgc cttgccggag cgcgcccact
     4021 ccttgacgcc gccctcgagg tggtagacgc gctggtagcc gaaccgctcg gccagcttgg
     4081 ccgcctgcaa gctgcgcacg ccccgggcgc agatgaacac ggtcgcgaca tcgcgcgcga
     4141 gcgccgcgtc ggggtccgcg cggagccgct ccagcggcac cagccgcgcg cccgcgatgt
     4201 ggcccgcctg ccactcgtcg ggatcgcgca catcgaccag atcgacgatc tgggccgatg
     4261 tgagctcggc gaggccggcc gccgtgaccg cttgaaccat gcctccaaca taacggatga
     4321 cctgatggtc ggcaaggggt ccgctcaagc ggaggcagcc tggatccgct tcaaggacgc
     4381 gaccagcgcg tcgatgtcct cgtgaacgtt gtagaaagcc agggacgcgc ggacggtgct
     4441 ctccaggccg taccgccgca ggatcggctg ggcgcagtgg tggccggacc gcaccgcgat
     4501 gccgtccttg tcgagcgcgg cgccgatgtc ctccgtgcgc tggccgtcga gcacgaacga
     4561 cagcacgccc gccttgtggc gcggggtgcc gaccatccgc agcccgggca cggtggcgag
     4621 ccgctcggtc gcgtagccga gcagctcgtg ctcgtagcgc tcgatcgtcg gcatgccgat
     4681 ccgcagcacg tagtcgaggg cggcgccgag ccccgccgcg tcggcgatgt tgccggtgcc
     4741 ggcctcgaac cgccacggcg gctgctggta cagcgtgcgc tcgaacgtga cgtcggcgat
     4801 catgttgccg ccgccctgcc acggcggcat cgcgtcgagc agggcgcgct tgccgaacac
     4861 cgcgccgatg ccggtcggcg cgaacacctt gtgcccggag aacacgtaga agtcgcaatc
     4921 gagcgcctgg acgtcgaccg gcatgtgcga gaccgcctgg gcgccgtcga gcaccacggt
     4981 cgcgccccag cggtgcgcga tctcgatcat ctcgcgcgcc ggggtcaccg tgccgagcgc
     5041 gttcgacacc tgggtgaacg cgaccagccg ggtgcgcgag cacaggagct tctcgtaggc
     5101 ctcgaggatc acgtcgccgc gctcgtcgac cggcgcgacg cgcagccggg ccccggccgc
     5161 ggcgcacagc tgctgccacg gcacgatgtt cgcgtggtgc tcgaggtggg tgaccacgat
     5221 ctcgtcgccg cgctcgacgt ggcgggcgcc ccacgccttc gcgatcaggt tgatgccctc
     5281 ggtcgcgccg cggacgaaca cgatctcgct cgccgatggc gcgttcaaga acttgcgcgt
     5341 ggtgtcgcgc gcgctctcgt aggcgtcggt cgcgcgcgcc gcgagcacgt gggccgcgcg
     5401 gtgcacgttg gagttctcgt gctcgtagaa gtaggtcagc cgatcgatca ccgcgtgcgg
     5461 cttctgcgtg gtcgccgcgt tgtcgagcca caccagccgc ttgccgccat gcacgcgctc
     5521 ctcgaggatc ggaaagtcgc ggcgcagcgc atacggatcg aaccccgtcc cgcccagatc
     5581 gcgaa
//
LOCUS       DASVOR010001074         4966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_186439,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001074 DASVOR010000000
VERSION     DASVOR010001074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4966
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186439"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VF516_20575"
     CDS             <1..799
                     /locus_tag="VF516_20575"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8110143.1"
                     /translation="YAIDSIDALRATPISTATNKVQTLGNLAQIKRVTGPANITHSDI
                     KRTFDVQANVEGTDLGSVSDAVDRIVAAERTAAPRGTTITVRGQVESMNSSFGGLLSG
                     LGFAILLVYLLMVVNFQSWLDPFIILMALPGALAGIVWILFLTGTTLSVPALIGAIMC
                     VGVATANSILVVSFANDQRLSRDARAAALAAGMTRLRPVVMTALAMVLGMLPMALGLG
                     EGGEQNAPLGRTVIGGLGLATLTTLLFVPVMYSLLRRKPPVHREVDV"
     gene            811..2130
                     /locus_tag="VF516_20580"
     CDS             811..2130
                     /locus_tag="VF516_20580"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8110144.1"
                     /translation="MLRGHQDAPVSPAAEAHAALAPAERETAEDLGFALPAPARTSRT
                     GVIVVLVLLLGGLLVFGLSQRGKAHERTALPTATVRATHVQVIKPKVIDSDHALALPG
                     TVRALEQTKIFPRVTGYVRRWLVDIGDKVTAGQLLVEIDTPDLDAQLAQARAQLGQAQ
                     AAVKQVTAQRDYSRSNSQRFESLADQKLVSKSQVEQTQAQASTDEANVAAAQANVAAQ
                     QANVRRLVETQGFARVVAPFAGTISTRTVERGDLVTEGTGTALYTLVATDPVRVFIEV
                     PQTVAPSVRTGVGATVACREFPGRKFAGKVTRAAGALDPDLHTMTTEVQVPNPDGTLL
                     PGMYVQAELTLPVPHRVVEIPATALYNDAQGLRVVVVDAQQKAHFAPITIERDTGATL
                     QVATGLAGDARVVKIAVPDLTEGDPLEVSEAAPAAGSGAPAAGSAAK"
     gene            2208..3026
                     /locus_tag="VF516_20585"
     CDS             2208..3026
                     /locus_tag="VF516_20585"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8110145.1"
                     /translation="MAMLEINGTSLFYEDTGPGSTGETLAFSHGLLWGTELFAPQLAA
                     LRGRYRCIAWDHRGQGQSAADHRDCIGMELVWQDAVQLIEALGNGPVHLCGLSMGGFV
                     AMRMAARRPDLVRSLILLETSADPEPIENVPRYRLLNRVTRLLGTSITRNKVAPIMLG
                     KTILTDKARRADVDRFAQLMGRRRDIWRAVNGVVDRGGVYHELSRITAPTLVVVGDED
                     VATPRAKAERIAGAIAGAKLVAIPRAGHSSPVEEPELVTAAIEQFLNTLTPARS"
     gene            3396..>4966
                     /locus_tag="VF516_20590"
     CDS             3396..>4966
                     /locus_tag="VF516_20590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110146.1"
                     /translation="MATILIVDDSLTVRIDLAEALAGAGFHTIHCSTIAEARVALRSH
                     PVALAILDIQLPDGDGIDLLAQIRKDPTLGELPVLLLSTEAEVKDRIRGIRGGANDFI
                     GKPYDTYHVIARVGQLVGAPPVHDLVLIIDDSATFRGELSEALDKIGFATATATGGNE
                     GLRMAATLRPTVIVVDGMMPDMDGASVIRRLRLDPGLRTTPCLLLTGSDAKDAEVHAL
                     EAGADGFIRKNDLGLIVARVRALLRSTSPLRGDADSLLAPKRILAVDDDPDYRGVLAD
                     RLRKRGYDVAHAASGEEAIQLLAVQSVDCILLDRAMAGIGGIETCRRLKSSAIVRDTP
                     LIILTASDQRDAVLEGLGAGADDFVSKVSGFDVLSARIQAQIRRKQIEDEQRRVRDQL
                     LRSELDAAEARAAKALAETRAAMAEELARANADLALANRELEAFSYSGSHDLRAPLRT
                     ISAFSHAIIEDLGGQLDDKSHDHIRRVLAATSRMSDLIDALLELSRISRAPIGRHRVD
                     LSQLAAVVLDELARR"
BASE COUNT          739 a         1829 c         1715 g          683 t
ORIGIN      
        1 gtacgcgatc gactcgatcg acgcgctgcg cgcgaccccg atctcgaccg ccacgaacaa
       61 ggtccagacc ctggggaacc tggcccagat caagcgggtg accggaccgg cgaacatcac
      121 gcactccgac atcaagcgca cgttcgacgt ccaggccaac gtcgagggca cggatctcgg
      181 ctcggtcagc gacgcggtcg atcggatcgt cgccgcggag cgaacggccg cgccccgggg
      241 cacgacgatc acggtccgcg gccaggtcga gagcatgaac agctcgttcg gcggcctgct
      301 ttccggcctg gggttcgcga tcctgctggt ctatctcctg atggtcgtga acttccagtc
      361 gtggctcgat ccgttcatca tcctgatggc gctgcccggc gcgctcgccg gcatcgtgtg
      421 gatcctgttc ctgaccggga cgacgctgtc ggtgcccgcg ctgatcggcg cgatcatgtg
      481 cgtcggggtc gcgaccgcca acagcatcct cgtggtgtcg ttcgccaacg atcagcggct
      541 gtcccgcgac gcgcgggccg ccgcgctggc cgccgggatg acccggctcc ggccggtggt
      601 gatgaccgcg ctcgcgatgg tgctcggcat gctgccgatg gcgctcggtc tcggcgaggg
      661 cggcgagcag aacgcgccgc tcggacggac ggtgatcggc gggctcgggc tcgccacgct
      721 gaccacgctg ctgttcgttc cggtgatgta cagcctgttg cgccggaaac cgccggtgca
      781 ccgcgaggtc gatgtatgat ccaggatccc atgctccgcg gccaccagga cgccccggtt
      841 tcccccgccg ccgaggcgca cgccgcgctg gctcccgccg aacgcgaaac cgccgaggac
      901 ctcgggttcg cgctgccggc accggcgcgc acgtcgcgca ccggcgtgat cgtcgtgctc
      961 gtgctcctgc tcggcggcct gttggtgttc ggcctgagcc agcgcggcaa ggcgcacgag
     1021 cgcaccgcgc tgcccaccgc caccgtgcgc gcgacccacg tccaggtgat caagcccaag
     1081 gtgatcgaca gcgatcatgc gctggccctg cccggcaccg tgcgcgcgct ggagcagacc
     1141 aagatcttcc cgcgggtcac cggctacgtc cggcgctggc tggtcgacat cggcgacaag
     1201 gtcaccgccg gccagctgct cgtcgagatc gacacgccgg acctcgacgc ccagctcgcc
     1261 caggcccgcg cccagctcgg ccaggcccag gccgcggtca agcaggtgac cgcacagcgc
     1321 gactactcgc ggtcgaacag ccagcgcttc gagtcgctcg ccgaccagaa gctggtgtcg
     1381 aagtcgcagg tcgagcagac ccaggcccag gcgtcgaccg acgaggccaa cgtcgccgca
     1441 gcccaggcca acgtcgccgc ccagcaggcc aacgtgcgcc ggctggtcga gacccagggg
     1501 ttcgcccgcg tggtcgcgcc gttcgccggc acgatctcga cccgcaccgt cgagcgcggc
     1561 gatctggtca ccgagggcac cggcaccgcg ctgtacaccc tggtggcgac cgatccggtc
     1621 cgggtgttca tcgaggtccc gcagaccgtg gcgccgagcg tgcgcaccgg cgtcggggcg
     1681 acggtcgcct gccgcgagtt ccccggccgc aagttcgccg gcaaggtcac ccgcgcggcc
     1741 ggcgcgctcg atcccgatct ccacaccatg acgaccgagg tccaggtccc gaaccccgac
     1801 ggcacgctgc tgcccgggat gtacgtccag gccgagctca ccctgccggt cccgcaccgc
     1861 gtggtcgaga tcccggcgac cgcgctgtac aacgacgccc agggcctgcg ggtcgtggtg
     1921 gtcgatgccc agcagaaggc gcacttcgcg ccgatcacga tcgagcgcga caccggcgcc
     1981 accctgcagg tcgcgaccgg gctcgccggt gacgcgcggg tggtcaagat cgcggtgccc
     2041 gacctgaccg agggcgatcc gctggaggtc agcgaggcgg cgcccgccgc cggctccggt
     2101 gcgcccgccg ctggctccgc cgcgaagtaa cgggcaccac accggcgcga gggcttgatc
     2161 gcgatccgcg cgcgccgtac gactgaggcg cacggaggtc cccgccgatg gcgatgctcg
     2221 agatcaacgg cacgtccctg ttctatgaag acaccggccc cgggtcgacg ggcgagaccc
     2281 tcgcgttcag ccatggcctg ctgtggggga ccgagctgtt cgcgccccag ctcgcggcgc
     2341 tgcgcggccg ctaccgctgc atcgcgtggg accatcgcgg ccagggccag agcgcggccg
     2401 atcaccgcga ctgcatcggc atggagctgg tgtggcagga cgcggtccag ctgatcgagg
     2461 cgctgggcaa cggcccggtc cacctgtgcg gtctgtcgat ggggggcttc gtcgcgatgc
     2521 gcatggcggc gcgccggccc gacctggtgc gatcgctgat cctgctcgag acctcggccg
     2581 accccgagcc gatcgagaac gtgccgcggt atcgcctgct caaccgggtg acccggctgc
     2641 tcggaaccag catcacgcgg aacaaggtcg cgccgatcat gctgggcaag acgatcctca
     2701 cggacaaggc gcggcgcgcc gacgtcgatc gcttcgccca gctgatgggc cggcggcgcg
     2761 atatctggcg cgcggtcaac ggcgtcgtgg atcgcggcgg ggtctaccac gagctgtcgc
     2821 ggatcaccgc gcccacgctg gtcgtggtcg gcgacgagga cgtggcgacg ccgcgggcca
     2881 aggccgagcg gatcgccggt gcgatcgccg gcgcgaagct ggtggcgatc ccgcgcgccg
     2941 gtcactcgtc gccggtcgag gagcccgagc tggtcaccgc cgcgatcgag caattcttga
     3001 acaccctgac gccagctcgt tcatgaacgg gtcatgaaac gcgcgtgaat cgagcatgga
     3061 gctgcgattc tcggattcgt gatcctcgta cgggattgaa cgtacagcag atcaggattc
     3121 tggactcaca ttcgtccctg cccgcgcctc gcgggcgcaa gcatgcgacg atgccctcgc
     3181 gcgacccaga ccctccagac atcttctcac gcaactcagc cgacgtcgcc gacctcgctg
     3241 accggctggt ccaggccacc tagcggagca ccatggagat ttctcgcgta cccccttgga
     3301 cgccggccgg gtcccccgcc gcgcatggcg ccgacgacga ccccgtctac gctttgcccc
     3361 aggaggtcct acgcttaacg ggcaagggct ccgacatggc aacgatcctc atcgtcgacg
     3421 acagcctgac cgtccgcatc gacctcgcgg aggcgctcgc cggggccgga ttccacacga
     3481 tccattgctc gacgatcgcc gaggcgcggg tcgcgctgcg cagccatccg gtggcgctgg
     3541 cgatcctcga catccagctg ccggacggcg acggcatcga cctgctggcc cagatccgca
     3601 aggatccgac gctcggtgag ctcccggtgc tgctgctgtc gaccgaggcc gaggtcaagg
     3661 accggatccg cgggatccgc gggggcgcca acgacttcat cggcaagccg tacgacacct
     3721 accacgtgat cgcgcgggtc gggcagctgg tcggcgcgcc gccggtccac gatctggtgc
     3781 tgatcatcga cgacagcgcg acgttccgcg gcgagctgtc cgaggcgctc gacaagatcg
     3841 ggtttgccac cgcgaccgcg accggcggca acgaggggct gcggatggcg gcgaccctgc
     3901 ggccgaccgt catcgtcgtc gacggcatga tgcccgacat ggacggcgcc agcgtgatcc
     3961 gccggctgcg gctcgacccc gggctgcgca ccacgccgtg cctgctgctc accggctcgg
     4021 acgccaagga cgccgaggtc cacgcgctcg aggccggcgc cgacggcttc atccgcaaga
     4081 acgacctcgg cctgatcgtc gcccgggtcc gcgcgctctt gcgctcgacc agcccgctgc
     4141 gcggcgacgc cgacagcctg ctcgcgccca agcggatcct cgcggtcgac gacgatccgg
     4201 actaccgcgg cgtgctcgcc gaccgcctgc gcaagcgcgg ctacgacgtc gcccacgcgg
     4261 cgtcgggcga ggaggcgatc cagctgctcg cggtgcagag cgtcgactgc atcctgctcg
     4321 accgcgcgat ggcggggatc ggcgggatcg agacctgccg ccggctcaag agctcggcga
     4381 tcgtgcgcga cacgccgctg atcatcctga ccgcgagcga ccagcgcgac gccgtgctcg
     4441 agggcctggg cgccggggcc gacgacttcg tgtccaaggt cagcgggttc gacgtgctgt
     4501 cggcgcggat ccaggcccag atccggcgca agcagatcga ggacgagcag cgccgggtcc
     4561 gcgaccagct gctgcgcagc gagctcgacg ccgccgaggc ccgcgccgcc aaggcgctcg
     4621 ccgagacccg cgccgcgatg gccgaggagc tcgcccgcgc caacgccgac ctggcgctgg
     4681 ccaaccgcga gctcgaggcg ttcagctact cggggtcgca cgacctgcgc gccccgctgc
     4741 gcacgatcag cgcgttcagc cacgcgatca tcgaggacct cggcggccag ctcgacgaca
     4801 agtcccacga ccacatccgg cgggtgctcg ccgcgacctc gcggatgtcg gacctgatcg
     4861 acgcgctgct cgagctgtcg cggatcagcc gggcgccgat cggccgccac cgggtcgacc
     4921 tcagccagct cgcggcggtc gtgctcgacg agctggcgcg gcgcga
//
LOCUS       DASVOR010001075        12933 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_303192,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001075 DASVOR010000000
VERSION     DASVOR010001075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12933
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303192"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VF516_20595"
     CDS             <1..538
                     /locus_tag="VF516_20595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110147.1"
                     /translation="VLLGTTVLAVGAGVARHEAITRAPPSTLAQHLAKNFPDGQVAWL
                     MKDAAYETFVVKAGTKNWGRCDDTNCYVVAAPAGVIHAAVTQSMHGGRHDPAALGKAL
                     GIPAANVEGPLRMITLDAKKSGVCARLPVESDPGIWKCRSPEDTDCFKVGGYTSGGLA
                     ELMIINAPVDKTTIEDVQ"
     gene            1574..3286
                     /locus_tag="VF516_20600"
     CDS             1574..3286
                     /locus_tag="VF516_20600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110148.1"
                     /translation="MRIGGWQLAFVVVSALLGMGSFCQVTKSGPPPPKTILDTPQSPR
                     TFDGDVRTVCGGKTFPVDHDVEWQPVVGGVDEAYAVTGLSGSVVESNCSGGDFVFTHP
                     MGPDWETYIAVDRQYAGVLAPANQSPHQLGAGKEFEIAQQKADEFGFYDGIMGTEIDQ
                     GMSQPRYHACSPATVDAECPASPTTPRCANRDPKTADRTVIFGRWIVDCGHPDFHTEI
                     HPPLLSVVGYATTDQRSDYTIEGRPFLTSQHFNGESWWQNAQESLAKGNAACMFVPPL
                     LPCPFNVDWSSTMLPPFAGTTTMVFYIRPSSPPPTPTSTMKMRGRVYLRPGVTFEASA
                     RDDSYQVVVTMNGDEYPVPDVAKYDGTIPFQLSDLGDACKVTNDRGSSQCEFFGLQAL
                     LEALWIGVHPLTPDNYARYRRLNVQQIWADEAANQPTGDPNVADFQVLPAKQSLFPIQ
                     GTFSVEWVGEPTGRTIAISPQELNFALSNTPSLPITLKASGTLPVTIRSFSIDGPNSG
                     DFTISADACTGHTLMPGNTCQITIRYAASGVGGRSVTLHVNDDAVGSPQAVPMSGLGW
                     VTMR"
     gene            3968..5044
                     /locus_tag="VF516_20605"
     CDS             3968..5044
                     /locus_tag="VF516_20605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8110149.1"
                     /translation="MALHSTLAARRDEVIRRWKEQVRGTLAPETMPHMELLDHLPRFL
                     DEIVASLREDAGLSSTTPCPEHSTTAAGHGEQRLRLGFSLDSVVREYGALRDAILATA
                     REAGDELSFRETQIVFDCVITGIACAVSEYTHQRDADLQRLANEHFAFIAHELRNPLS
                     SATLAFDQLIAKGIVPSDTREVSAIGRSLQRVSELVDQTLRIARVASGIELRRQYTTL
                     GALFEDVELGAISEANARGVAIKVVLGHDERVQLDVRLVHSAVSNLVRNAVKYTRQQS
                     TVELRGSAENGRVVVEIEDCCGGLEPGKVEQAFAPFVRLDTKQGGFGLGLAIAKQAVA
                     AHGGTIRVQNIPGKGCIFVLELPT"
     gene            5275..5523
                     /locus_tag="VF516_20610"
     CDS             5275..5523
                     /locus_tag="VF516_20610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110150.1"
                     /translation="MTRQRPASAVDADNGRHRLGRARDALASEQLAAAHPPLGLTWTC
                     ASGEIRAMSVTSACAPLVVSSARRVAPGATRERRTGPF"
     gene            complement(5676..5798)
                     /locus_tag="VF516_20615"
     CDS             complement(5676..5798)
                     /locus_tag="VF516_20615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110151.1"
                     /translation="MTEAVRKAGAADLVRALPGGYDQIVALHAQRQGSLQIGWQ"
     gene            complement(5905..6972)
                     /locus_tag="VF516_20620"
     CDS             complement(5905..6972)
                     /locus_tag="VF516_20620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8110152.1"
                     /translation="MPEIRAAALEDRSDILTLIREMIPGADVEARWRWLYETNPAGRA
                     LTWIATHGGQLAGCTSFFPLRLWLDGEVVRAALGGDGYVRPAFRRNGIGAALHDASRS
                     AMPAHQIGCMYGAPGAMNVTPLKRGGSREIGQVVRWARPVRFAALGFRAPLLGRLAVA
                     TSSSAADLEPMRPRDARVDDVWVEARRDLRLAAVRDSAFYTWRFIEAPARRQRAFVIV
                     ECGRPIGACALELTRGGNVLTIIDLLTVPGAWRAGLRAIVRNAAEQTRAQVVDMKLFA
                     NDGRLHHLWRSGFTEREGKPFLCMIPSGGDRRFLDPDRWHYSGADSDLDTLEAPPPPP
                     ASRRSTMTGLKTQRPTIPLPV"
     gene            7434..8582
                     /locus_tag="VF516_20625"
     CDS             7434..8582
                     /locus_tag="VF516_20625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8110153.1"
                     /translation="MDFAIAWVQALVAGQALPRRARAPVCPPARPDGGLGVDRIDSPR
                     SYAPACRPRHGPRALRVCWERRSAARAGTSDSMEGITRSLRTAARGLARRLWAFKVPV
                     GAAIAVFAASLVAQQVAPSVAVLVAVALGVLVVLVEQRRQRAWIDGRFKEVVSDVAQI
                     QPLLELEARLQPRRPLPPLRGYAIAPDFALMLTTLIAEEQPELVVETGSGVSTLVIAY
                     ALEKLGRGRVVALELDAGYAAKTRDEIERHRLSAYATVVHAPLAPIEIDGKRYTWHSL
                     SALEGLDHIDLVVDDGPPRYLGEMLRYASLPTFAERLSQRGLFVLDCVADEERETLAL
                     WKRRHPEFAQEHLDTKKGNVILRRAPVAAPAPAPLTVSPLEEPKPQMP"
     gene            complement(8633..9046)
                     /locus_tag="VF516_20630"
     CDS             complement(8633..9046)
                     /locus_tag="VF516_20630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110154.1"
                     /translation="MRGDAGRPASEDHTLKVWELAMYTCRITHRGDAGYLAVAVSAPD
                     VTAWDASGAVWFFDMPWRPNLLRDGDDHLPQRRSFQETEPVSQRALMKHTILFLAANP
                     LGADRRDLDREARALRAELAREVALVGSRAAAPKT"
     gene            complement(9374..10147)
                     /locus_tag="VF516_20635"
     CDS             complement(9374..10147)
                     /locus_tag="VF516_20635"
                     /inference="COORDINATES: protein motif:HMM:NF005451.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8110155.1"
                     /translation="MAVAVPKPRSLRDQVSPEEWAARVDLACAYRLVAHHGWTWMIYN
                     HISARVPGHDDQFLLNPFGLHYTEVTASNLVKIDLGGKILDGSPYPINDAGFTIHSAI
                     HGARHDVAAVLHTHTEAGAAVSALDVGLLPFSQDALLFYNRIAYHDFEGIALDLDERS
                     RLVRDLGPHHAMILRNHGLLTCGRTIAEAFMFMYQLERVCRSQLAMMAAGGKIRLPPP
                     EVCEKTALQYSCNRGEPAGVHEWPHWVRMMDQLDPSYRN"
     gene            10205..11863
                     /locus_tag="VF516_20640"
     CDS             10205..11863
                     /locus_tag="VF516_20640"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8110156.1"
                     /translation="MVGTNDLDFDFVVIGSGFGGSVSACRLAEKGYRVAVIEMGKRWT
                     AEDFPKTTWHLRRWLWRPRLKLFGFYDMRPFRHVMILCGNAVGGGSITYANTLLSPPD
                     RVWQDGSWKGLADWQREMPAHYAEARRMLGVTENRILGDADRMLQKAAQAQGVGDTFY
                     KTDVAVYFGDGPGQPHPDPYFGGEGPPRSSCIGCGGCMVGCRYNAKNTLDKNYLYFAE
                     KRGAKVMAETRVVDLRPIGGPGADGSAGYELTVERSTAWLFKQRRTLTARQVVLAASS
                     LGTMDLLMRTKQRGSLPAVSDRLGHDVRTNAESIVGVRFPGSEHDMSKGIAIGSGIHI
                     DPFTHVEATRYSRGSDVLGLIGTLLVNGKGWRRIGTWLASAVRHPIRFARAAWPFGFA
                     RQTLIFLVMQTIDATLRMRLRRRWFWPFTRLLCSEGSRIPTNIPQANAFVERAAAQLG
                     GTPITSWFEILFDMPMTAHCIGGCVMGTDPAHGVIDAQHRVFGYHGLFVVDGSAVGAN
                     LGVNPSLTIAALAERAMSFIPAKRSAEASSSPRDHGVANRVEHA"
     gene            11990..12886
                     /locus_tag="VF516_20645"
     CDS             11990..12886
                     /locus_tag="VF516_20645"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX8110157.1"
                     /translation="MPRNAARVTTRLSGGGETHRGHVRESNQDVILVEPDLGLYAVLD
                     GMGGANAGDVAARMAGDKLLDCVRQKSRIRRRSPHRLLQLALDTAAIEVFRAAEERLE
                     YRGMGTTVVACLVVGPTRVIIGHAGDSRAYLLRGGELIALTRDHTVAQRLLDAGQRNI
                     EEITRHQLTRNLGREYGVRPDVLEQALEPGDRLLLCSDGLYGGASMGAIRRVLGSRDT
                     PDRVAHSLVALALKGRASDNISAIVIAVDGSGVRAATRRRPSRAQASSGERAPASPRR
                     RAAARTGPRRGPPRGGGPARGG"
BASE COUNT         2025 a         4511 c         4451 g         1946 t
ORIGIN      
        1 ggtgctgctc gggacgaccg tgctcgcggt cggggcgggc gtggcccgtc acgaggccat
       61 cacgcgcgcc ccgccgtcga ccctcgccca gcacctcgcg aagaacttcc ccgacggaca
      121 ggtcgcctgg ctgatgaagg acgccgccta cgagaccttc gtggtcaagg ccggcaccaa
      181 gaactgggga cgctgcgatg acaccaactg ctacgtggtc gctgcccccg cgggggtcat
      241 ccacgccgcg gtcacgcagt ccatgcacgg cggccgtcac gatccggcgg cgctgggcaa
      301 ggccctcggc ataccggcgg cgaacgtgga aggcccgctc aggatgataa cgctcgacgc
      361 caaaaagagc ggcgtctgtg cccgcctgcc cgtcgagagc gatcccggca tctggaagtg
      421 caggagtccc gaggacaccg actgcttcaa ggtcggcggc tacacctcgg gcggccttgc
      481 ggagctcatg atcatcaacg cacccgtcga caagaccacg atcgaagatg tccagtaacc
      541 ggaccttcgc acgagcgtta gacgatcgtc ccaggtcacg gcacgacgtg tcgccaccgg
      601 cgagcgcggg taccagatcc accggcgtca ccgggatcca gggacccacc cggtggcgcg
      661 cagacggttc ttcgcctcga cggtgccgca gcggcacgcg cacgagctca agttcgcggt
      721 gcgaaccgag cgaagccgag catgaggctc gatcggagca gagtcaggtt gcgttcactg
      781 tggggcgtac gacccgcatc gcgagcgcgg gggccggcgc gtgggcggag gtcgggcagc
      841 ggcggcggcc gaccgtcttt ggcccgcgcg tccaacggct cgctgcgcgg tggcgagagc
      901 ctgcgcggtc gggccggtcg aacgccccga tggatccgct ggcattctcc tactttataa
      961 atgccagttg acataactac tattctgcga cgtgacccta tatcccaaaa atagagcagg
     1021 gtaaggagat gcaatgtaca acgtgcctcc tgccgtcgca agccgctggg gacagggctg
     1081 cggctctcga ccaagacgcc gttctcgagc gcgacagagc gccacgctac cagtcgccga
     1141 gcgagccggg atccatcggc accgccccag acccgggcgg ttccgtcgtc gctggcggtg
     1201 acgacgcgtg cgaaagcctg cggtgcttgc cagcaggggg gcccagcccc cgagctgcgt
     1261 agatttactc taaggggccc aggagcaacg gcgcgggagc ttacggtagg cggcccaacc
     1321 tccgagccga tgcggcgaag cagcatgagg gcaggagacg tgcgagcccg cggcagcgtg
     1381 gtgaacatcg ccaatcaccg ctcggtgatc atgtgcacgg ccgcgcgcat cgatcgtggc
     1441 cctggccacc agggctgagg acctcgacct cctccttgcc cagcacggtt cgcaggcccg
     1501 atcggtcgcg cgcccccgtg agaatagacc gcgcatcacg acccgccgtg cgcggcggtg
     1561 atacgatgcg ggcatgcgca tcgggggctg gcagctcgcg ttcgtggtgg tatcggcgtt
     1621 gctcgggatg ggttccttct gtcaggtgac gaagtcggga ccgccgccgc cgaagacgat
     1681 cctcgacacg ccgcagtcgc cgcggacgtt cgatggcgac gtccggacgg tgtgcggcgg
     1741 caagacgttc cccgtcgatc acgatgtcga gtggcagccg gtcgtcggtg gcgtcgacga
     1801 ggcgtatgcg gtgacaggcc tgtccggctc cgtggtggag tcgaactgtt cgggcggcga
     1861 cttcgtgttc acgcacccga tggggccgga ttgggagacg tacatcgccg tcgaccggca
     1921 gtacgcgggc gtgctcgccc ccgcgaacca gagtccgcac cagctcggcg ccggcaagga
     1981 gttcgagatc gcgcagcaga aggccgacga gttcgggttc tacgacggga tcatgggcac
     2041 cgagatcgat cagggcatgt cgcagccgcg ctaccacgca tgctcgccgg cgaccgtcga
     2101 cgccgagtgt cccgcgtcgc cgacgactcc gcgctgtgcg aaccgggatc ccaagaccgc
     2161 cgaccgcacg gtgatcttcg ggcgctggat cgtcgactgc ggccaccctg acttccacac
     2221 ggagatccat ccaccgctcc tcagcgtcgt cgggtacgcc accaccgacc aacgatcgga
     2281 ctacacgatc gaaggccggc ccttcctcac cagtcagcac ttcaacgggg agagctggtg
     2341 gcagaacgcg caggagagcc tcgcgaaggg aaacgcggcg tgcatgttcg tgccgcctct
     2401 cttgccctgc ccgttcaacg tggactggag ctcgaccatg ttgccgccgt tcgcgggcac
     2461 gacgacgatg gtcttctaca tccgaccgtc gagcccgccg ccgacgccga cctctacgat
     2521 gaagatgcgc ggccgcgtct acctccggcc cggtgtgacc ttcgaggcct ccgcacgcga
     2581 tgacagctat caggtggtcg tcaccatgaa cggcgacgag tacccggtcc ccgatgtcgc
     2641 gaagtacgac ggcaccattc cgttccagct ctcggatctc ggcgatgcgt gcaaggtcac
     2701 gaacgaccgg gggagcagcc agtgcgagtt cttcggcctg caagcgctgc tggaggcgct
     2761 gtggatcggc gtccatccac tgacgccgga caactatgcg cgctaccgcc ggttgaacgt
     2821 ccagcagatc tgggccgacg aggcggccaa ccagccgacc ggcgacccca acgtcgccga
     2881 cttccaggtg ctgccggcga agcaatcgct cttcccgatc cagggcacgt tcagcgtcga
     2941 gtgggtcggt gagccgaccg ggcgcacgat cgcgatctcg ccgcaggagc tgaacttcgc
     3001 gctctcgaac acgccgtcgc tgccgatcac gctgaaggcg agcggcacgc tgccggtgac
     3061 gattcgctcg ttctcgatcg acggaccgaa ctccggcgac ttcacgatca gcgccgacgc
     3121 ctgcaccggc cacacgctca tgccggggaa cacgtgtcag atcacgatcc gctacgcggc
     3181 gagcggcgtc ggtgggcgaa gcgtcacgct tcatgtgaac gatgacgcgg tgggatcgcc
     3241 gcaagcggtc ccgatgtcgg gcctcgggtg ggtgacgatg cggtgactcg tctcggcgcg
     3301 aacgcgcgtg atagcgtccg cgcatggtgc gtcgtgacga cgaaggacac cccgctgggg
     3361 atcccggcga gaaaccctcg tcacgctgcg gtgatccagc gggtgtaagt cccgtctcgg
     3421 taagcggtaa cgcgccgagt agcaggctcc cggcgcgggg agctgacggc cgatgtggtt
     3481 cgcgatccgg acggggacct tggcgaagtc gtccacgaat cgcccgaggg cgcgcaacgc
     3541 gcagagctgc acagcgaagc gttgccgatg ctcgggaccg cggcccttcc ggacctcctc
     3601 gagatcgtgg gcgtcttggg cgtccgagcc gccgcgaacg cgaaccgcaa cgccgcgtcg
     3661 gttgcgaacg gccgcggagc cgccgccgct actgcgatcg ctgcgcgtgt gatcccgtgc
     3721 gatcgatgtc gatcgctgcg tttcgactgc ttccaagccg tggcgcggtc gaacgtggcg
     3781 cctacagcct accccttccg ttggaccttg gttgcgagga ctttcccgaa cctgaccgct
     3841 tcgttcgatc gatagctgcc gcgatcgctg agcatgagga cctcttggtc acgacgaccg
     3901 gctgagagtt tcactgtccg cgctatcccg gcggtgccgc ggactggttt ccgtgccaca
     3961 cctccgcgtg gcgctccatt cgacactcgc ggcacggcgc gacgaggtga ttcggcggtg
     4021 gaaggaacag gtccgaggca cccttgctcc cgagacaatg cctcacatgg agctgctcga
     4081 tcatcttccg cggtttctcg acgagatcgt cgcctcgcta cgcgaggacg ctgggctatc
     4141 atcgactacg ccatgcccag agcacagcac caccgccgcc ggacatggcg agcagcgact
     4201 ccgcctcggg ttcagcctcg actcggtcgt gcgtgagtac ggagctctgc gcgacgcgat
     4261 cctcgccacg gcgcgagaag ccggcgatga gctcagcttc cgagagacgc agatcgtgtt
     4321 cgattgcgtt atcaccggca tcgcgtgcgc ggtctccgag tacacgcacc agcgcgacgc
     4381 cgacttgcag cgcctggcga acgagcactt cgcgttcatc gcccacgagc tgcgcaatcc
     4441 gctttcgtcc gcaacattgg ctttcgacca gctcatcgcc aagggcatcg ttccgagcga
     4501 cacacgcgag gtcagcgcga tcgggcgcag cctgcaacgc gtgagcgagc tggtcgacca
     4561 gacgctgcgg atcgcgcgtg tcgcatccgg aatcgagttg cgtcggcaat acacgacgct
     4621 cggcgcgctg ttcgaggacg tcgagctggg cgccatctcc gaagccaatg cgaggggcgt
     4681 cgcgatcaaa gtcgtgctcg ggcacgacga gcgtgtgcag ctcgacgtgc gcctcgtaca
     4741 ctccgcggtc agcaacttgg tgcgcaacgc ggtgaagtac acgcgtcagc agagcaccgt
     4801 cgagctacgc ggcagcgccg agaacgggcg cgtcgtggtc gagatcgagg actgttgcgg
     4861 cggactcgaa cccgggaagg tcgaacaggc attcgctcca ttcgtgaggc tcgacaccaa
     4921 acagggcggc ttcggtctcg ggctagcgat cgccaagcaa gccgtcgctg cgcatggcgg
     4981 aacgatccgc gtgcaaaaca tccccggcaa gggttgcatc ttcgtgctgg agctgccgac
     5041 ctgatcggac atgatctcga cccggcgcaa tcatctcgag ggcgatcccg acgagcccgg
     5101 cacgttcgca ggaccgggca taggctcgat acgtggccgg cccggcggtc gtgcgagcgt
     5161 cggcaacacg gcctcgagcg cgtcccgccg cgacaaccgg gccgcgtcgt cgctcgccgc
     5221 gcgcgtgaag tcccgcgctg cgcccgggtc gtacgacccg ccgtcaacca agccgtgacc
     5281 cgccagcgac ccgccagcgc ggtcgacgcc gacaacggtc gccatcgcct cggccgggcc
     5341 cgcgatgccc tcgccagcga gcaactcgcc gccgcccacc cgccgctcgg gctgacgtgg
     5401 acatgcgcca gcggcgagat ccgcgcgatg tccgtcacct cggcctgcgc gccgctcgtg
     5461 gtctcgagcg cgcgcagggt cgcgccggga gccacccgcg aacgccgcac gggcccgttc
     5521 taagctgacc cgcgcgcggc tccaagggcg ccgccacgcg gccctccacc gtccgccgcg
     5581 gtgcggctta ctgccaacgc atctgcaacg agtccacctc agccgagcgt tcactgccaa
     5641 ccgatctgca acgaggctgc ctcggcccag ccggtctact gccaaccgat ctgcaacgag
     5701 ccctgtctct gggcgtgcaa cgcaacaatc tggtcgtagc cgccgggcag cgcgcgcacg
     5761 agatcggcgg cgccggcctt gcgcacggcc tcggtcatcg cggcctcatc gaggcggccc
     5821 ggatcgccga atgaactttg gtaggatctc ctccgtcgac accctccaag gaacttcgcc
     5881 ttcaccgggc ggacggcccc atcctcacac cggcagcggg atggtcgggc gctgggtctt
     5941 gagccctgtc atcgtgctgc ggcgcgatgc gggcggcggc ggtggcgctt cgagcgtgtc
     6001 gagatcggaa tcggcaccgc tgtagtgcca gcggtcggga tcgaggaacc ggcggtcgcc
     6061 gccgctcggg atcatacaca gaaacggctt gccctcgcgc tccgtgaacc ccgagcgcca
     6121 caggtgatgg agacgaccgt cgttcgcgaa aagcttcatg tcgaccacct gtgcgcgggt
     6181 ctgctcggcg gcgttgcgca cgatcgcgcg cagcccggcg cgccaggcgc ccggcacagt
     6241 cagcaggtcg atgatggtga gcacgttgcc gccgcgggtc aactcgagcg cacacgcgcc
     6301 gatcggccgg ccgcactcga cgatgacgaa cgcgcgctgc cgccgggctg gagcctcgat
     6361 gaatcgccac gtatagaatg cggagtcgcg cactgccgca agtcggaggt cacgccgggc
     6421 ctcgacccag acgtcgtcga cccgcgcgtc gcgcggccgc atcggctcga ggtcggcggc
     6481 gctgctcgac gtggcaaccg cgagcctgcc gagcagcggc gcgcgaaacc ccagcgcggc
     6541 gaagcggacc ggacgagccc agcgcacgac ctggccgatc tcgcgcgatc cgccgcgctt
     6601 gagtggggtc acgttcatcg cgccgggtgc gccatacatg cacccgatct gatgcgccgg
     6661 catcgcgctg cgcgacgcgt catgcagcgc ggcaccgatg ccgttgcggc gaaacgccgg
     6721 ccggacatag ccgtcgccac cgagtgccgc gcgcacgacc tccccgtcga gccacagccg
     6781 caacggaaaa aacgacgtac agccggcgag ttggccgccg tgcgtggcga tccaggtcag
     6841 cgcccggcct gcgggattgg tctcgtacag ccagcgccaa cgcgcctcga catcggcccc
     6901 gggaatcatc tcgcgaatca gggtgaggat gtccgagcgg tcctcgaggg ccgcggcgcg
     6961 tatctccggc atggaggacg ccagtagcaa ccagcgttcc tcctgaccca tcgcgctaag
     7021 aggcttcccg gccggccaag gcgaactccg cgtgacctgc cgccacgatc acccgtcgcg
     7081 cagtgtgtcg agctgactcg ttactggtgc gcggtcaccc gggcgccggt cgatggacgg
     7141 cgggtaacgg agcgtggcga ccgcaagggc ctccacgggc cagctcggcg tgccgcgcga
     7201 tggggagcat gaagtggcgc ggttcgtcgt agtcgatgtt gtcgatgccg gttccgatct
     7261 cggttgtgcc gatgaccacg ccgcgcgcgg ggtcgatcgg ggcgagaccg gaccggggtt
     7321 gatcttgcgc tgaggaggcg gcgtgcaacc tcgtcaagct cgcgaacgac ggcgtcttcc
     7381 cccaggtctg cgtcgacccc ggcgcgctcc acaccaacgt cgtcggccgc aacatggact
     7441 tcgcgatcgc atgggtgcag gcactcgtcg ccgggcaggc gctcccacga cgtgcccgag
     7501 caccggtctg cccacctgca cgccctgacg gaggcctggg cgtggatcgt atcgactcgc
     7561 cgcgctctta cgcccctgca tgccgtccaa ggcatggccc gcgcgctctt cgggtgtgtt
     7621 gggagcggcg cagcgccgcc cgcgctggta catccgacag catggaaggg atcacccgaa
     7681 gcttgcgtac agccgcgcgg gggctcgcgc ggcgtctctg ggccttcaag gtgccggtcg
     7741 gggcagccat cgcggtgttc gccgcgtcgc tcgttgccca gcaggtcgcg ccgtccgttg
     7801 cggtgctggt cgcggtcgcg ctgggcgtgc tggtcgtcct cgtcgagcag cggcgccaac
     7861 gcgcgtggat cgacgggcgg ttcaaggagg tcgtctccga cgtcgcgcag attcagccgc
     7921 tgctcgagct cgaagcgcgg ctccagccgc ggcgcccgct gccgccgctg cgcggctatg
     7981 ccatagcccc cgatttcgcg ctcatgctga ccacgctcat cgctgaggag cagcccgagc
     8041 tggtggtcga gaccgggagc ggcgtctcca cgctcgtgat cgcctacgcc ctcgagaagc
     8101 tcggccgcgg ccgcgtcgtc gccctggagc tcgacgccgg ctacgccgcg aagacccgcg
     8161 acgagatcga gcgtcaccgg ctctccgcct acgcgacggt cgtgcacgcg ccgctcgcgc
     8221 cgatcgagat cgacgggaag cgctacacgt ggcactcgct ctccgcgctc gagggcctcg
     8281 atcacatcga cctcgtcgtc gacgacggcc cgccccgcta cctgggcgag atgctgcgct
     8341 acgcgtcgct cccgacgttc gccgagcgcc tcagccagcg cggcctcttc gtgctcgact
     8401 gcgtcgccga cgaggagcgc gaaaccctcg cgctctggaa gcgccgccat cctgagttcg
     8461 cccaggagca cctcgacacc aagaagggca acgtgatcct gaggcgtgcg cccgtcgccg
     8521 cgccggcgcc ggctcccctg acggtgtccc cgctcgagga gccgaagccg cagatgccgt
     8581 gatcgcctct cggcgctcac ccgcgcccat gatgcggttc ctcgagctcc ggtcaggtct
     8641 tgggggcggc ggcgcgggaa cctaccaacg ccacttcgcg cgccagctcg gcgcggagcg
     8701 cgcgcgcttc acgatcgagg tcccgccgat ccgcgccgag cggattggcc gcgaggaaca
     8761 aaatggtgtg cttcatgagt gctcgctgcg aaacgggctc ggtctcctgg aacgatctac
     8821 gttgtgggag gtggtcatcg ccgtcgcgga ggaggtttgg gcgccagggc atgtcgaaga
     8881 accagacggc accagaggca tcccaggcgg taacgtcagg cgcgctgacg gcgaccgcga
     8941 ggtaaccggc atcgccgcga tgggtgatgc ggcacgtgta catcgccagc tcccagacct
     9001 tgagcgtgtg gtcctccgac gccggccgtc cggcgtcacc gcgcacgcgt tcacctcacg
     9061 ggtgtggcct gcgagcgtgg ccaccgcgcg cccgcgcgca gttcccagac cttgagcgtt
     9121 ttgtcccacg acgccgagac cacgtgccgg ccgtccggcg tcaccgcgca cgcgttcacc
     9181 tcacgagtga ggcctgcgag cgtggccacc gcgccccggc ggatgaagtt atgcggcacg
     9241 gtgtcgcctg ctgcctcgcg tcgagctagc caggccgcga gcagcgcgct cttgcctatc
     9301 ccgggcccgc cagtcaccac gagcgcagcg agcggaagtg gatcgaccga gacgtcgtgg
     9361 tgaatgatgc gcgctagttg cgatacgacg gatcgagctg gtccatcatg cggacccagt
     9421 gcggccactc gtggacgccg gccggctcgc cgcggttgca gctgtactgc agcgcggtct
     9481 tctcgcacac ctccggcggc ggcagccgga tcttgccgcc cgccgccatc atcgcgagct
     9541 gcgagcggca cacccgctcg agctggtaca tgaacatgaa cgcctcggcg atggtgcgcc
     9601 cgcaggtcag caggccgtgg ttgcgcagga tcatcgcgtg gtgcgggccg agatcgcgca
     9661 ccagccgcga ccgctcgtcg agatcgagcg cgatgccctc gaagtcgtgg tacgcgatcc
     9721 ggttgtagaa cagcagcgcg tcctggctga acggcaacag cccgacgtcg agcgccgaca
     9781 ccgcggcgcc ggcctcggtg tgggtgtgca gcacggccgc gacgtcgtgg cgcgcgccgt
     9841 ggatcgccga gtggatcgtg aagcccgcgt cgttgatcgg gtacggcgag ccgtcgagga
     9901 tcttgccgcc gaggtcgatc ttgaccaggt tcgacgccgt gacctcggtg tagtgcagcc
     9961 cgaacgggtt gagcaggaac tgatcgtcgt ggcccggcac gcgcgcgctg atgtggttgt
    10021 agatcatcca ggtccagccg tgatgggcga ccaggcggta cgcgcacgcg aggtcgaccc
    10081 gcgccgccca ctcctcgggg ctgacctgat cgcgcagcga gcgcggcttg ggaacggcga
    10141 cggccatgga cattcctccg gctgcgcact ctagccgatc cccgcgctgg gtcatactgt
    10201 ggtgatggtg ggcacgaacg acctcgactt cgacttcgtg gtgatcggct cgggcttcgg
    10261 cggcagcgtg tcggcgtgcc ggctcgccga gaagggctac cgggtcgccg tcatcgagat
    10321 gggcaagcgg tggaccgccg aggactttcc aaagaccacg tggcacctgc ggcgctggct
    10381 gtggcggccg cggctcaagc tgttcgggtt ctacgacatg cggccgttcc gccacgtgat
    10441 gatcctgtgc ggcaacgcgg tcggcggcgg ctcgatcacc tacgccaaca ccctgctgtc
    10501 gcctcccgat cgggtgtggc aggacggctc gtggaagggc ctggccgact ggcagcgcga
    10561 gatgccggcg cactacgccg aggcccgccg catgctcggc gtgaccgaga accggatcct
    10621 cggcgacgcc gatcgcatgc tgcagaaggc ggcccaggcg cagggcgtcg gcgacacgtt
    10681 ctacaagacc gacgtcgcgg tctacttcgg cgatgggccc ggccagcccc accccgatcc
    10741 gtacttcggc ggcgagggcc cgccgcgcag ctcgtgcatc ggctgcggcg gctgcatggt
    10801 cggctgccgc tacaacgcga agaacacgct cgacaagaac tacctgtact tcgccgagaa
    10861 gcgcggcgcg aaggtgatgg ccgagacccg cgtggtcgac ctccggccga tcggcgggcc
    10921 cggcgccgac ggctcggccg gctacgagct caccgtcgag cgctcgaccg cgtggctgtt
    10981 caagcagcgc cggacgctga ccgcgcgcca ggtcgtgctc gcggcgtcgt cgctcggcac
    11041 gatggacctg ctgatgcgga ccaagcagcg cggctcgctg ccggcggtgt ccgaccggct
    11101 cggccacgac gtccggacca acgccgagtc gatcgtcggc gtgcggttcc cgggcagcga
    11161 gcacgacatg tccaagggca tcgcgatcgg ctcgggcatc cacatcgacc cgttcaccca
    11221 cgtcgaggcg acccgctact cgcgcggctc cgacgtgctc ggcctgatcg ggacgctgct
    11281 ggtcaacggc aagggctggc gccggatcgg gacctggctg gcctcggcgg tccgccaccc
    11341 gatccggttc gcgcgcgcgg cgtggccgtt tggcttcgcc cgccagacgc tgatcttcct
    11401 ggtgatgcag acgatcgacg cgaccctgcg catgcggctg cgccggcgct ggttctggcc
    11461 gttcacgcgc cttctgtgca gcgagggcag ccggatcccg accaacatcc cgcaggccaa
    11521 cgcgttcgtc gagcgcgccg cggcccagct cggcggcacc ccgatcacgt cgtggttcga
    11581 gatcctgttc gacatgccga tgaccgcgca ctgcatcggc ggctgcgtga tgggcaccga
    11641 tccggcgcac ggcgtgatcg acgcccagca ccgggtgttc ggctaccacg ggctgttcgt
    11701 cgtcgacggc tcggcggtcg gcgccaacct cggcgtcaat ccgagcctga cgatcgccgc
    11761 gctcgccgag cgcgcgatga gcttcatccc ggcgaagcgg tccgccgagg ccagctcgag
    11821 tccgcgagac catggcgtcg cgaatcgcgt cgagcatgcg tgatcatcgc agcgtcgtgc
    11881 cgttccgatc gccgatgctg caactccagg ccagctggct cacgctcgcc ggccgaacgc
    11941 gcggggtcct ggtggcgaag cgagcgcgat ccgggctacg gtcccgccga tgccccgcaa
    12001 cgccgctcgc gtgacgaccc gcctgtcggg cggtggcgag acccatcgcg gccacgtcag
    12061 agagagcaac caggacgtga tcctcgtcga gccggacctg gggctgtacg cggtcctcga
    12121 cgggatgggc ggtgccaacg cgggcgacgt cgcggcgcgc atggcgggtg acaagctcct
    12181 ggactgcgtc cggcagaagt cccgcatccg gcggcgctcg ccgcaccggt tgctgcagct
    12241 cgcactggat accgccgcca tcgaggtgtt tcgagccgcg gaggagcggc tggaataccg
    12301 cgggatgggc acgaccgtcg tcgcgtgcct ggtcgtcggg ccaacgcgcg tgatcatcgg
    12361 tcatgccggc gacagccgcg catacctgct gcgcggcggt gagctgatcg cgctgacgcg
    12421 tgaccacacg gtcgcgcagc gcctcctgga cgccggacag cggaacatcg aggagatcac
    12481 ccggcaccag ctcacccgca acctggggcg tgaatacggc gtgcgacccg atgtgctgga
    12541 gcaggcgctc gagccgggcg atcgcctgct gctgtgctcg gacggcctgt acgggggcgc
    12601 atccatgggc gcgatccggc gtgtcctggg gtcccgtgac acgcccgacc gcgtcgcgca
    12661 cagcctggtc gcgctggcgc tcaaggggag agcgtcggac aacatctcgg ccatcgtgat
    12721 cgccgtggat ggcagcggag tgcgcgctgc aacgcggcgg cggccgtccc gcgcccaggc
    12781 tagctccggc gagcgcgctc cggccagccc tcggcgccga gcagcggcac gaaccggacc
    12841 tcgccgaggt cctcctcgtg gaggtggtcc ggcgcgcggc gggtgatccg gaccagccgc
    12901 tggtgctcga cgctgccgtg cggcagcacc agg
//
LOCUS       DASVOR010001076         4253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_419359,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001076 DASVOR010000000
VERSION     DASVOR010001076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4253
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419359"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VF516_20650"
     CDS             complement(<1..1101)
                     /locus_tag="VF516_20650"
                     /inference="COORDINATES: protein motif:HMM:NF015659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pullulanase-associated domain-containing
                     protein"
                     /protein_id="HEX8110158.1"
                     /translation="MASCSGSGEPPATAEGAAALSDSSSSASQGGLGSDDFCNATQLS
                     TVLTAVPPSPGGAAPINVTVHYNRPDGQYAGWGLHVWQVNDANQFVADYPGVTFPQPL
                     LPAGFDGYGAVFQIEAAKFTAANAAGFGFIVHQGNTKDPDPDRFWQFAGGGEIWLRSG
                     DTTIFTSNPLGATPDLTTVRVHYKRFDASYAQWGLHLWATSGLDVSRLPAGLHIDDFG
                     NPVPLSAMSGFTARPDGSEVVFDLPVLNPTGDASRTALEFIIHGMPSNPSGGVNNKDG
                     WSSNIHVNYAALKITAQVGEIWLVQDTPQVFTSVPSTRTASTIDARAMWLSRGLVRWS
                     RTDSAGAFRLYHSATGQIVARQGAPVTGADGAL"
     gene            complement(1338..3749)
                     /locus_tag="VF516_20655"
     CDS             complement(1338..3749)
                     /locus_tag="VF516_20655"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8110159.1"
                     /translation="MCSRPRVSVLIALAAALAGCASATTATGQLEVGHPTSGAFSAVN
                     ESGWGTATWPQGASFVSGEGSNLQVGVYAAHATRVLLEIYPSATGASAQFDYVMAKGS
                     DGIWRAQLASVPGKTLYAFRAWGPNWTFSSSWAPGSSAGFVSDVDASGNRYNPNKVLA
                     DPYARELSHDKTSPALIAAGLDGGIYGSGGDTYHGAPRRNFDTGPWAPKSVALANATS
                     TGTRPAIAARDSVVYETHVRGLTAHASAMNLTTLLAGIPGFSGVVNVSSCRGTYQAAG
                     LMAPYLQGLGVNVIELLPVHESDNDTIPLDHSGGNYWGYVTDGYFAPDRHYACDRSLG
                     GATTEFKTMVKAFHDHGIEVWLDVVYNHTGEGGNWDSTKQTAAVTSFRGFDNIDFYEL
                     PGTDHASYFDTTGVGNNFNVASAVGRRLVLDSLKYWANQMGVDGFRFDLACELGRDAA
                     PSYSFNAGAQLYKDIASFASAGNLDVVAEPWDIGAYGVGQFPSGWGEWNGKFRDATRR
                     FFNGDLSGSNGATYADVFYGDFNDYNDQGGAQKTVNFIVAHDGFTLADLVSYTAKTNA
                     SRTWPFGPSDGGSDSNDSSDSGQDPSLRRQRFRSLLTWEMFSRGVPMLVAGDELARTQ
                     NGNNNPYNVDAPGTWNNYGMIATDSPHLTATGVSGEAYHNNFGTDGHADGKNALFQFA
                     RQLINLRRSSRALRQADYTMPITFAKNDGSSGFDSHSDRAVRIQLGGSSVGDTDYLLY
                     VNNWTGTVTFTPPAPPAGKSWKRIIDTAAWAEPNDNFWTDASAASITGTYDAHPWSIV
                     VLKAL"
BASE COUNT          618 a         1395 c         1553 g          687 t
ORIGIN      
        1 cagcgcgccg tcggcgccgg tcaccggtgc gccctggcgc gcgacgatct ggccggtcgc
       61 cgagtggtac agccggaacg cgccggcgct gtcggtcctg gaccagcgca ccaggccgcg
      121 cgacagccac atcgcgcgcg cgtcgatggt cgacgccgtg cgcgtgctgg gcaccgacgt
      181 gaacacctgc ggcgtgtcct ggaccagcca gatctcgccg acctgcgcgg tgatcttgag
      241 cgccgcgtag ttgacgtgga tgttcgacga ccagccgtcc ttgttgttga ccccgccgct
      301 ggggttgctg ggcattccgt ggatgatgaa ctcgagcgcg gtccggctcg cgtcgccggt
      361 cgggttcagc accggcaggt cgaacacgac ctcggagccg tcgggccgcg cggtgaaccc
      421 cgacatcgcc gacagcggca ccgggttgcc gaagtcgtcg atgtgcagcc cggcgggcag
      481 ccggctgacg tcgagcccgc tggtcgccca caggtgcagg ccccactgcg cgtagctggc
      541 gtcgaaccgc ttgtagtgga cccgtacggt ggtcagatcg ggcgtggcgc ccagcgggtt
      601 ggaggtgaag atcgtcgtgt cgcccgaccg gagccagatc tcaccgccgc ccgcgaactg
      661 ccagaaccga tcgggatcgg ggtccttggt gttgccctgg tggacgatga agccgaaccc
      721 ggccgcgttc gcggcggtga acttcgcggc ctcgatctgg aacaccgcgc cgtagccatc
      781 gaacccggcg ggcagcagcg gctgcgggaa cgtgacgccc ggatagtcgg cgacgaactg
      841 gttggcgtcg ttgacctgcc agacgtggag tccccagccg gcgtactgac cgtctggccg
      901 gttgtaatgg accgtgacgt tgatcggcgc cgcgccgccc ggagacggcg gcaccgcggt
      961 cagcaccgtg ctcagctgtg tcgcgttgca aaaatcatca ctgcccagcc cgccctgaga
     1021 tgcactcgac gacgaatcgc tcagcgcggc cgcgccttcc gcggtggccg gcggttctcc
     1081 agagccgctg cagctcgcca tcaagatcgt ggagagcagg ggaaaccaga atcggtcgcg
     1141 cgatgatgca ctgcgtatca tgggtcgtct ttcgatgagc ggtggagagg cgacgtgcga
     1201 ggggcgtgcc agtatgcgcg gtatgcgcaa ccgcaggagc tgcggtactc gcgacgacag
     1261 aggatcgagt ctcgcgctac gattgcgcag cgtcgatcgg gagtcggtga gctttgcgcg
     1321 aggcagctat acgcagatca cagcgccttg agcacgacga tcgaccacgg gtgtgcgtcg
     1381 taggtgcccg tgatcgacgc cgcgctggcg tcggtccaga agttgtcgtt gggctcggcc
     1441 cacgcggccg tgtcgatgat ccgcttccag ctcttgccgg cggggggcgc cggcggcgtg
     1501 aacgtcaccg tgccggtcca gttgttgacg tacagcaggt aatcggtgtc accgaccgac
     1561 gagccaccga gctggatccg caccgcgcga tcggagtgcg agtcgaaccc ggacgagccg
     1621 tcgttcttgg cgaacgtgat cggcatcgtg tagtcggcct ggcgcagcgc gcgcgacgac
     1681 cgccgcaggt tgatcagctg gcgcgcgaac tggaacagcg cgttcttgcc gtcggcgtgg
     1741 ccgtcggtgc cgaagttgtt gtggtaggcc tcgcccgaca ccccggtcgc ggtcaggtgc
     1801 gggctgtcgg tggcgatcat cccgtagttg ttccaggtgc cgggcgcgtc gacgttgtac
     1861 gggttgttgt tgccgttctg ggtccgcgcg agctcgtcgc cggcgaccag catcggcacg
     1921 ccgcggctga acatctccca ggtcaagagg ctgcggaacc gctggcggcg cagcgagggg
     1981 tcctggcccg agtccgagct gtcgttggag tcgctgcccc cgtccgacgg accgaacggc
     2041 caggtccggc tcgcgttggt cttggcggtg tagctcacca ggtcggcgag cgtgaagccg
     2101 tcgtgggcga cgatgaagtt gacggtcttc tgcgcgccgc cctggtcgtt gtagtcgttg
     2161 aagtcgccgt agaacacgtc ggcgtaggtc gcgccgttcg agccgctgag gtcgccgttg
     2221 aagaaccggc gggtcgcgtc gcggaacttg ccgttccact cgccccagcc gctcgggaac
     2281 tggccgacgc cgtaggcgcc gatgtcccag ggctcggcga cgacgtcgag gttgccggcg
     2341 ctcgcgaacg acgcgatgtc cttgtagagc tgcgcgccgg cgttgaagct gtacgacggc
     2401 gccgcgtcgc ggccgagctc gcacgcgagg tcgaaccgga acccgtcgac gcccatctgg
     2461 ttcgcccagt acttcaggct gtcgagcacc agccgccggc cgaccgccga tgccacgttg
     2521 aagttgttgc cgacgccggt ggtgtcgaag tacgacgcgt gatcggtgcc gggcagctcg
     2581 tagaagtcga tgttgtcgaa gccccggaag ctggtcaccg cggcggtctg cttggtgctg
     2641 tcccagttgc cgccctcgcc ggtgtggttg tagaccacgt cgagccagac ctcgatgccg
     2701 tgatcgtgga acgccttgac catggtcttg aactcggtgg tcgcgccgcc cagcgaccgg
     2761 tcgcacgcgt agtggcgatc cggcgcgaag tagccatcgg tgacatagcc ccagtagttg
     2821 ccgccggagt ggtcgagcgg gatcgtgtcg ttgtccgact cgtgcaccgg cagcagctcg
     2881 atcacgttga cgccgaggcc ctggaggtag ggcgccatca gcccggcggc ctgataggtg
     2941 ccgcggcagc tcgagacgtt gaccacgccg gagaaccccg ggatcccggc gagcagggtg
     3001 gtgaggttca tcgccgaggc gtgcgcggtc aggccgcgga cgtgggtctc gtagaccacc
     3061 gagtcgcgcg cggcgatcgc cggcctggtg ccggtgctcg tcgcgttggc gagcgcgacc
     3121 gacttgggcg cccacggccc ggtgtcgaag ttgcggcgcg gcgcgccgtg gtaggtgtcg
     3181 ccgccgctgc cgtagatccc gccgtcgagc ccggcggcga tcagcgccgg tgacgtcttg
     3241 tcgtgcgaca gctcgcgggc gtacggatcc gcgagcacct tgttcgggtt gtagcggttg
     3301 ccgctggcgt cgacgtcgct gacgaacccg gccgacgacc ccggggccca gctcgacgag
     3361 aacgtccagt tcggccccca cgcgcggaac gcgtacagcg tcttgcccgg caccgaagcg
     3421 agctgggcgc gccagatccc gtcgctgccc ttggccatca cgtagtcgaa ctgcgcgctc
     3481 gctcccgtcg ccgacgggta gatctcgagc agcacgcgcg tcgcatgcgc cgcgtagacc
     3541 ccgacctgca ggttcgagcc ctcgccggac acgaagctcg cgccctgcgg ccacgtcgcg
     3601 gtcccccagc cgctctcgtt gacggccgag aacgcgccgc tcgtcgggtg gccgacctcg
     3661 agctgcccgg tcgcggtggt cgccgaggcg cagccggcca gcgcggccgc gagggcgatc
     3721 aggacagaga cgcgcggacg tgagcacatg ggagtctccg ggagcgggcg catggtgcgc
     3781 catcgccatg cgagaccatg gccagcgaac gggcaggtct cgcgggcgag cgatctcgat
     3841 ctgggccgat ggtacgacca cttcgacgca gcgtgagggc cgcggaacta ttgggccgcg
     3901 gggatggacg ttgccaccga ggggcgtttc gcaacgccgc ggatccgccg cacgaggctg
     3961 tgcgggccac cggcccggtc gcggcgttgc ctttgtcgac gcgattgcgg caagtggcgt
     4021 tgccggagtg ccttcacagc ggatgtgatt ttcgtgagcc gggatgcaga agcgtgaagc
     4081 ggacgtacgg tccggcaacc aagcgaccga tgcagcgcac gtggtcgcat gggctgatcg
     4141 tatcgagctt gctggtacag ggcgcggccc gggcggatga atcaccgccg ccggcgcccg
     4201 cggcggccga ccccgccgcg ccggcagatc ctggcacgac ccgcagcgag ctg
//
LOCUS       DASVOR010001077         3440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_99069,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001077 DASVOR010000000
VERSION     DASVOR010001077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99069"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF516_20660"
     CDS             complement(<1..325)
                     /locus_tag="VF516_20660"
                     /inference="COORDINATES: protein motif:HMM:NF039283.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX8110160.1"
                     /translation="MRAAEPQRAIEDHALIGDLHTAGLVAKDGSIDFLCLPDFDDGST
                     FASLLGTPENGRWRIAPLRPAHAVQRRYRGNTLILETEFENDEGVVRLIDFMPIRRSR
                     HQAPRV"
     gene            716..1147
                     /locus_tag="VF516_20665"
     CDS             716..1147
                     /locus_tag="VF516_20665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110161.1"
                     /translation="MYFVIVTMVAAIALAVGMSVLFRVGRRFGARNLPEAGDAWGRRG
                     RASDLRIALHDLLIAFTFSGAAERFDARRRMITGRATCDTDAGPARVHQDVRPGSNAN
                     GFPTDTPPKVVYGQLFALALLRRCLQGVEWRAARPAPGSTV"
     gene            1408..1962
                     /locus_tag="VF516_20670"
     CDS             1408..1962
                     /locus_tag="VF516_20670"
                     /inference="COORDINATES: protein motif:HMM:NF016269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HEX8110162.1"
                     /translation="MTEVVMKKLIFAAVAAALAPACAHTPKTPQEEATLESKTSSTLS
                     EMRQKDPSIDQALRSAYAYAIFPEVGKAGFIAGGASGAGILYEQGRPTGNVRISQASF
                     GAQAGAETFAELVLLHNQDEVNKLKNGSFDLGANASAVVLKAGGAAASATARGTSVFV
                     MPKGGAMVDISVAGQKIKFEPFAG"
     gene            complement(2237..2776)
                     /locus_tag="VF516_20675"
     CDS             complement(2237..2776)
                     /locus_tag="VF516_20675"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8110163.1"
                     /translation="MAKLVFGMSQSLDGYVDDAAGTLCMPAPGPALFRHFIDVVAGQA
                     GAIYGRRIYDVMRYWDVDRPEWDVAEREFAAAWRRRPKWIASRTLVSVGPNATLIRGD
                     LDAFVRDLKATVDGELDVAGPELAAAVAPLIDEYRLYFRPYVLGGGKPYFAGARPPLR
                     LVATDRIGEDVVRLTYVPA"
     gene            3145..3366
                     /locus_tag="VF516_20680"
     CDS             3145..3366
                     /locus_tag="VF516_20680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110164.1"
                     /translation="MARISVQRQPRRNLRPQLASRSKWHRIEALLRNRAFIEDYIHAR
                     DAWRQGNMIAFPPGTYWLRRFANVVVADT"
BASE COUNT          562 a         1124 c         1189 g          565 t
ORIGIN      
        1 tcacgcgggg cgcttgatgc cggctgcgcc ggatcggcat gaagtcgatc agccggacca
       61 caccctcgtc gttctcgaac tcggtctcga ggatcagcgt gttgcctcga taccgccgct
      121 ggaccgcgtg cgcgggcctt agcggcgcta tcctccagcg tccgttttcc ggcgttccta
      181 gcagcgacgc gaaggtggag ccgtcatcga aatcgggaag acacagaaag tcgatcgagc
      241 cgtccttcgc gacgagcccc gccgtatgaa gatccccgat cagtgcgtgg tcctcgatcg
      301 cacgctgcgg ctctgccgcg cgcacagcag cgtggcgcac ttgcgatgct ggtgaagtag
      361 cagtcacgga agcctcctta ttgtgtctgc cttggttgca atgcaagacg cgtcgctgcg
      421 aagccgcgga gcgcacgtcg cacgcgaagg cgcaacgatc cgttgacgta cgacgtacgc
      481 gcacgatcgc tatccgctgc cgcctcgatc aggtcggtgc actgtgatac gtaggtttcg
      541 ctgatcgtcg gcgactgcgt cgtcgtcgag ccagcgctgc ccgcctggcc cgtacgtgca
      601 agccttaagc gtgctcagcg agcgcatgac gatgtctcgc gagtacgcga caccgtagcg
      661 gcacgcacat acgtgcactg gatcggcgca tgcaatgatg caatgacttg aaccgatgta
      721 tttcgtgatc gtgacgatgg tggctgctat tgccttggcc gtcggcatgt ccgtgctgtt
      781 tcgcgtcggg cgccggttcg gcgcgcgtaa tctcccggag gccggtgatg cgtgggggcg
      841 gcgcggtcga gccagcgatc ttcgcattgc attgcacgac ctgctcatcg cattcacgtt
      901 ctcaggtgcg gccgagcgat tcgacgcacg tcgacgcatg atcaccgggc gcgccacttg
      961 cgacaccgat gctggtcctg cccgcgttca ccaagatgtt cgaccaggca gcaacgcgaa
     1021 tggtttccct acagatacac ccccgaaggt cgtatacggg cagttgttcg cgctcgcctt
     1081 gcttcgtcgc tgcttgcagg gcgtggaatg gcgcgccgca agaccggcgc ctggttctac
     1141 ggtgtagcgt actctcttgt tatgtcagtc gtcgtgtatg tgatcatcga cctcgagaat
     1201 cctcgcgggg gcctgatccg cgtcggaaac ctcggccgcg tgctggtcga gctgcagtcg
     1261 gaactgggga cgcagtccca ccatcgacaa cgccgcggat cgatagcatg cgtctcaccg
     1321 ttatcggccg cgtcgagtgc gtaccacggc ggtctgaccg tcacgttcca gccgaggcag
     1381 aacaggtccg gcagatgcat cggaccggtg acggaggtag tcatgaagaa gctgatcttc
     1441 gccgcggtcg ccgcggcact ggctccggcg tgcgcgcaca cgccgaagac tccgcaagag
     1501 gaggccacac tcgagagcaa gacgagctcg acgctctccg agatgcgcca gaaggatccg
     1561 agcatcgatc aggcgctacg tagcgcgtac gcgtacgcga tcttccccga ggtcggcaag
     1621 gccggcttca tcgccggtgg tgcctcgggc gcgggcatcc tgtacgagca gggccggccg
     1681 accggcaacg tcaggatctc gcaggcctca ttcggcgcgc aggcgggcgc cgagacgttc
     1741 gccgagctcg tgctgctgca caaccaggac gaggtcaaca agctaaagaa cggctcgttc
     1801 gacctcggcg cgaacgcgag tgcggtggtg ctgaaggccg gcggcgccgc ggccagcgcc
     1861 acggcgcgcg gtacgagcgt gttcgtgatg ccgaagggcg gcgcgatggt cgacatctcg
     1921 gtcgccggtc agaagatcaa gttcgaaccg ttcgcaggct gagccgcgcc acacgcgcgc
     1981 tccgatcgcg cacgtctggg cttcgcggat aggcgtgcgg ctggagctca tgcgcggagt
     2041 ccgttcgatc tggcggtccc gatcttgctg ccccgtgtat caatggacct ctcgacggct
     2101 gacgtgtgcg gacgctcgat ggcgggcggt tgggcggcgt tcgtggtcgc ccggagcgct
     2161 gctggctgcc ccagacgctg gggcgcggcg agcgcgccgg ctgagccgca cggggggccg
     2221 tcgctcgagt agcgcctcac gcggggacgt aggtcagccg gaccacgtcc tcgccgatgc
     2281 ggtccgtggc gacgaggcgc agcggcggcc gcgcgccggc gaagtacggc ttgccgccgc
     2341 cgagcacgta gggcctgaag tagagcctgt actcgtcgat cagcggcgct acggcggccg
     2401 caagctcggg cccggcgacg tcgagttcgc cgtcgaccgt cgccttcaga tcgcgcacga
     2461 acgcgtcgag gtcgccgcgg atcagcgtcg cgttcggccc gaccgacacg agcgtgcgcg
     2521 acgcgatcca cttcggccgc cgtcgccagg cggcggcgaa ctcgcgctcc gccacgtccc
     2581 actccggccg atcgacgtcc cagtagcgca tcacatcgta gatgcgccgg ccgtagatgg
     2641 cgccggcttg gccggcgacg acgtcgatga agtgacgaaa cagcgccggg cctggcgccg
     2701 gcatgcagag cgtacccgcc gcgtcgtcga cgtagccgtc gagcgattgc gacatcccga
     2761 acacgagctt tgccatgagc ggatggtaac tcgtgcgggc gcggacgaga cccgaccgac
     2821 cgcgacgccg tgatggacaa gctggtctac acagccacca acccggtgca ggaccacctg
     2881 gttgaccggg tgcgccactg gtctggccgc gctcctggcg ggacgcagct tgcgtgcgcg
     2941 gcggccgcgg cacttcttcc ggccggacgg gcccatgccc gaggaggtcg agatgcggct
     3001 cacgatcccg cccgcgctcg ggcccgaggc ggaggtgctc gaggagctgc gggaacgcgt
     3061 ccaggcggtc gaagccgagc acgacgcgga gcgccagcgg accggcaggc gggtgctcgg
     3121 acggcgcgcc gtgctccagc aatcgtggcg cggatttccg tccagcgtca accgcggcga
     3181 aacctccgac cgcagctggc cagccggagc aagtggcacc ggatcgaggc gctgctgcgc
     3241 aaccgcgcgt tcatcgagga ctacattcac gctcgggatg cgtggcgcca gggcaacatg
     3301 atcgcgtttc ctccgggcac ctactggctc cgccggttcg cgaacgtggt cgtcgcagac
     3361 acgtgaggtg agtaaagctc gacgcagatg aaggatgacg gcgcgtgacc cccgaggcgc
     3421 gtatgcgtct cggaagggag
//
LOCUS       DASVOR010001078         3932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_69112,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001078 DASVOR010000000
VERSION     DASVOR010001078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69112"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VF516_20685"
     CDS             complement(<1..851)
                     /locus_tag="VF516_20685"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8110165.1"
                     /translation="MRSFLWPIAALLVLPSCGGSDGPVPAPFGLDQRPSNTTCLARPR
                     PVVNTGVKLQPMWSTLTFQEPLFLLQAPGDDRWFVVQREGKVRVFARDATDDSQVRDF
                     ATVTVNSAGEGGLLGMAFHPQWPTRREVYLYYTRTPVAGDPAPVCAAAPPPPPSGLVS
                     ILARFQSQDAGATLDATPDEILRVGQPFTNHKGGTIEFGLDGMLYLGLGDGGSGNDPC
                     GSGQNKNSLLGKILRFDVNAPKGMYNVPADNPFAGVSGSRGEIWSYGHRNPFRWHFDR
                     ASGDLWVG"
     gene            complement(937..1062)
                     /locus_tag="VF516_20690"
     CDS             complement(937..1062)
                     /locus_tag="VF516_20690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110166.1"
                     /translation="MANVGQKFAQCVQHPREREEFFRDRMADELDAVPRANVRSS"
     gene            1170..3905
                     /locus_tag="VF516_20695"
     CDS             1170..3905
                     /locus_tag="VF516_20695"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX8110167.1"
                     /translation="MVSLVSLGLAACTGDQGPPGGQGSQGPPGNQGPPGDQGPPGDQG
                     PPGNQGPPGTVVTTGTLSGRITDGVAGDPLANVSVEFADLGGIALAPAVSTDADGRFS
                     ATVDAGLVIVSVDKQFYTSPGSLLTSVSIGRTTSMTFTMNESASGRPSLALAAPGDDV
                     GYGVTIPLTATASDPNGDSLTYTWKNTTAPLLGAVTGSGSSGSLTMPTMAAAFAFRPD
                     PTNPGQFISGYTLENRFGIVPILPDTRGQITASVTVSDGRGQSATATLTLNAASVQGG
                     TRNVPVGQRIYVNSGHDGANGWTLVAPGGSPATLDDPTRRTPSFVVDRAGSYTVTEGA
                     NTMTVTAGTWRGMIAGGSGNAVTQDSMCMVCHGNQFGNGLVIPDMFTPWLGTGHATMF
                     TRGINGVASDHYSGACFGCHTVGYDPGIANNGFDDVAAAVNWNMPVMGTTSWANLVVN
                     APNVAKLANIQCESCHGPQDSAAHTRTWDANQKSQPFLSPRIAYAAENCATCHAAGAH
                     HLYSEWTTTDATGMGHSNRAAISFGASATGLNASCGRCHTAQGYTLYAGVLAQGKVAL
                     NSIPAATLATVTPANAEPVTCVACHDPHDATNPNQLRFYGDTPNLPSGFAGRGMGKGA
                     LCLTCHNSRNGAQTGSDTLTFLHEDGETYNSGNPTGFSAPHQADQGDVFTGHNAYFLG
                     ASLPMTSKHAAVQDTCVGCHMTLQPKGYLSHGAPTASGHLFRIEEADQQKLCANCHGS
                     AVDGAGIQGQVESQLAALAAKMGNAVKARVNGFPGGLVRVRAWDPATDLNSSTSASNL
                     VLDVVANPVTAVDVEEIHGQIGFILHFATPITVPFVDAAGNPVPSKAMTSFGVQLGAI
                     KDNAATPAALYLLSGNLVRAGWNYFLVEGDQSKGLHNPSFVTAVLNTTLSKDLSN"
BASE COUNT          642 a         1428 c         1337 g          525 t
ORIGIN      
        1 ccgacccaga ggtcgcccga cgcgcgatcg aagtgccagc ggaacgggtt gcggtggccg
       61 tacgaccaga tctcgccgcg cgacccggac acgccggcga acgggttgtc ggcggggacg
      121 ttgtacatgc ccttcggcgc gttgacgtcg aaccgcagga tcttgccgag cagtgaattc
      181 ttgttctggc ccgagccgca cggatcgttg cccgagccgc cgtcgccgag gccgaggtac
      241 agcatgccgt cgaggccgaa ctcgatcgtg ccgcccttgt ggttggtgaa cggctggccg
      301 accctgagga tctcgtcggg cgtggcgtcg agcgtggcgc cggcatcctg gctctggaac
      361 cgcgcgagga tcgagaccag cccgctcggc ggcggcggtg gagccgccgc gcacaccggc
      421 gccggatcgc cggccaccgg ggtccgcgtg taatagaggt agacctcgcg ccgggtcggc
      481 cactggggat ggaacgccat gccgagcagg cccccctccc cggcggagtt gacggtgacg
      541 gtggcgaagt cgcggacctg gctgtcgtcg gtcgcgtccc tggcgaacac gcggaccttg
      601 ccctcgcgct ggacgacgaa ccaccggtcg tcgccggggg cctgcagcag gaacagcggc
      661 tcctggaacg tcagcgtgga ccacatcggc tgcagcttca cgccggtgtt gacgaccggg
      721 cgcggccggg cgaggcaggt cgtgttggag gggcgctggt cgagcccgaa cggcgccggc
      781 acggggccgt cgctgcctcc gcacgacggc aggacgagca gcgcggcgat cggccacagg
      841 aagctacgca tggccgcgac gataccgccg cggcgcggtg cgagcccgac gcctcacctg
      901 cgcatctgat cccgtccggc tcgcgtgcga tctcgatcac gacgaacgga cgttcgcgcg
      961 ggggaccgcg tcgagctcgt cggccatgcg atcccgaaaa aattcctcgc gttcgcgagg
     1021 gtgctgcacg cactgcgcga atttctggcc gacgttcgcc attcgcgcat gaaaaacgac
     1081 cacttgtgcg caggttcact ccttgcactg ggcccgcagc aagggtcttt cgcttggagg
     1141 cagcaatgag gttgctcgga tatggaacct tggtctcgct cgtttcgctc gggctcgcgg
     1201 cgtgcaccgg ggatcaaggg ccgccaggcg ggcaggggtc gcaggggccg ccgggcaatc
     1261 aagggccgcc gggcgatcaa ggcccaccgg gcgatcaggg cccaccgggc aatcaaggac
     1321 cgcccggcac cgtggtcacg accggtacgc tcagcggccg gatcaccgac ggcgtcgcgg
     1381 gtgacccgct cgccaacgtg agcgtcgagt tcgccgatct cggcggcatc gcgctggcgc
     1441 cggcggtctc gaccgacgcc gacggccggt tcagcgcgac cgtcgacgcg ggcctggtca
     1501 tcgtgtcggt cgacaagcag ttctacacct cgcccggatc gctgctcacg tcggtgtcga
     1561 tcggtcggac gacgagcatg acgttcacga tgaacgagtc ggccagcggc cggccgagcc
     1621 tcgcgctcgc ggcgcccggc gatgacgtcg gctacggcgt gacgatcccg ctgaccgcca
     1681 cggccagcga tccgaacggc gactcgctca cctacacgtg gaagaacacc accgcgccgc
     1741 tgctcggcgc ggtcaccggc agcggctcgt ccggctcgct gacgatgccg accatggccg
     1801 ccgcgttcgc gttccggccc gatccgacca accccgggca gttcatctcg ggctacacgc
     1861 tcgagaatcg gttcggcatc gtcccgatcc tccccgacac gcgcggccag ataaccgcga
     1921 gcgtgacggt gtccgacggc cgcggccaga gcgcgaccgc cacgctgacc ctcaacgccg
     1981 cgagcgtgca gggcggcacc cgcaacgtgc cggtcggcca gcggatctac gtcaacagcg
     2041 gtcacgacgg cgccaacggc tggacgctgg tcgctccggg gggctcgccc gcgacgctcg
     2101 acgatccgac ccgccgcacg ccgtcgttcg tggtcgaccg cgcgggcagc tacacggtca
     2161 ccgagggcgc caacacgatg acggtcaccg cgggcacctg gcgcggcatg atcgccggcg
     2221 gctccggcaa cgcggtcacg caggacagca tgtgcatggt ctgccacggc aaccagttcg
     2281 gcaacgggct ggtcattccc gacatgttca cgccgtggct cggcaccggg cacgccacga
     2341 tgttcacccg cgggatcaac ggcgtggcgt ccgaccacta ctcgggcgcg tgcttcggct
     2401 gccacaccgt cggctacgat ccgggcatcg ccaacaacgg cttcgatgac gtcgcggcgg
     2461 cggtgaactg gaacatgccg gtgatgggca cgaccagctg ggccaacctg gtggtgaacg
     2521 caccgaacgt cgccaagctc gccaacatcc agtgcgagag ctgccacggc ccgcaggact
     2581 cggcggccca cacccggacc tgggacgcca accagaagag ccagccgttc ctgtcgccgc
     2641 gcatcgcgta cgccgcggag aactgcgcga cctgccacgc cgccggcgcg caccacctct
     2701 actccgagtg gacgaccacc gacgcgaccg gcatgggtca ctccaatcgc gccgcgatca
     2761 gcttcggcgc ctcggcgacc gggctcaacg ccagctgcgg ccgctgccac accgcgcagg
     2821 gctacacgct ctacgccggc gtgctcgccc agggcaaggt cgcgctcaac tcgatcccgg
     2881 ccgcgacgct ggcgaccgtg accccggcca acgccgagcc ggtgacctgc gtggcctgcc
     2941 acgatccgca cgacgcgacc aaccccaacc agctgcggtt ctacggcgac acgccgaacc
     3001 tgccgtccgg gttcgccggc cgcggcatgg gcaagggcgc gctgtgcctg acctgccaca
     3061 acagccgcaa cggcgcacag accggcagcg acacgctgac gttcctgcac gaggacggcg
     3121 agacctacaa cagcggcaac ccgaccggct tcagcgcccc gcaccaggcg gatcaggggg
     3181 acgtgttcac cggccacaac gcgtacttcc tcggcgccag cctgccgatg acttcgaagc
     3241 acgccgcggt ccaggacacc tgcgtcggct gccacatgac gctgcagccc aagggctacc
     3301 tgtcgcacgg cgcgcccacc gcgagcggcc acctgttccg catcgaggag gccgaccagc
     3361 agaagctgtg cgccaactgc cacggcagcg cggtcgacgg cgccgggatc cagggccagg
     3421 tcgagagcca gctcgccgcg ctggcggcga agatgggcaa cgcggtcaag gccagggtca
     3481 acggcttccc gggcggcctg gtccgggtcc gggcctggga ccccgcgacc gacctgaact
     3541 ccagcacgtc ggcctccaac ctggtgctcg acgtcgtcgc caacccggtg accgcggtcg
     3601 acgtcgagga gatccacggc cagatcggct tcatcctgca cttcgcgacc ccgatcaccg
     3661 tgccgttcgt cgacgcggcc gggaacccgg tgccgagcaa ggcgatgacc agcttcggcg
     3721 tgcagctggg cgcgatcaag gacaacgcgg ccaccccggc ggcgctgtac ctgctgtccg
     3781 gcaacctggt ccgcgcaggc tggaactact tcctggtcga gggcgatcag agcaagggtc
     3841 tgcacaaccc gagcttcgtc accgccgtgc tgaacaccac gctgagcaag gacctgtcga
     3901 actagtgctg caacgctgct agccacgcac gt
//
LOCUS       DASVOR010001079         3637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_507565,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001079 DASVOR010000000
VERSION     DASVOR010001079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507565"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..572
                     /locus_tag="VF516_20700"
     CDS             411..572
                     /locus_tag="VF516_20700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110168.1"
                     /translation="MPGHPPEGNDGRVPLLIDDLPPFWGRWGRIYWLVAGLLAVETLV
                     FWWLTRWAS"
     gene            548..2305
                     /locus_tag="VF516_20705"
     CDS             548..2305
                     /locus_tag="VF516_20705"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEX8110169.1"
                     /translation="MADPVGVVTGLAAVDWAVLGATIAAIVGYGAWRTFGVRDADAYV
                     RGEALRWPTIGLGIMATQASAITFLSLPGQAYADGMRFVQFYFGLPLATILISAVFVP
                     VYHRLRVRTAYEYLEHRFDLRVRLLGALLFLIGRGLAAGITIYAPSIILSQILGWPLH
                     STIWGIGALVVLYVLLGGSRAVSVTQRQQMIVILIGLVIAAAIVIARLPDQVSLGGAV
                     RIAGALGRINPVSFQLDFNSRYNFWAGITGGFFLQLSYFGTDQSQVQRYLSGRSVAEM
                     RLGLLFNGMFKVPMQFLILFIGVMVFVFFLFTRPPMHFDAPTLARVEAARPAEVHALE
                     ARFDRAFEAQRGAALAYLAARGTPDEPAVRARLATSAAEIDRVRADAKQLIQTTLPEA
                     QTRDTDYVFLTFVLQYIPHGLVGLLLAVILCAAMSSVAGELIALGSTTAVDFYLRLRE
                     ALGRPPGSRTRDLRVSQLAMVAWGVIVLGIASAASLFANLIEAVNILGSLFYGTILGL
                     FMVALFLRRITATPVLIGAIVAELLVVALFLTSTLGYLWFNAIGCATVVVVSALLQTV
                     LPAASSPASAPASSAPSSG"
     gene            2413..3438
                     /locus_tag="VF516_20710"
     CDS             2413..3438
                     /locus_tag="VF516_20710"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX8110170.1"
                     /translation="MRALLTTLILLGSATAYAQLKLPEPSPAATVEQTIGITEVKVVY
                     HRPEIKGRTIWGQLVPYNEPWRAGANENTLVSFSSDVKVGGKPLKAGTYGLHMIPTAK
                     EWTIAFSTMTTAWGSFTYDPKEDALRVTVTPRTEAASEERLEYDFEDVTDGKATLRLR
                     WEKLAVPIAIEVDTPKVVMASVRGELRGLAGFRAETLNQAAQYWVQHGGNLDEALKFV
                     DTALQRGGQYQSHMLKAQILEKKGNAAGAADERKKAEAVATENDLNRSAYVLMNDKKV
                     DEAIKLLVAATQRFPEAWNLQDSLGEALALKGDKAGAQAAYSKALALAKDPVQKKRIE
                     GAIAKLK"
BASE COUNT          528 a         1288 c         1252 g          569 t
ORIGIN      
        1 cctcgggctg gttggcgcgc tcgtggctgg ccggtcgcgc aggccggcgg tgggctcggg
       61 gcgcccggcg tgataacgct gatgtacagt aggagctcat gatggcgcgc cacggggttc
      121 gtagagcatc gcttcgaggc ccggaggctt cgtcaagacc tcgccacaca ccacgggatc
      181 cagcgcaacc aggcggatct cggcgggata cggtcggccg cacgtgcccg agagagttgc
      241 agagcgccga cggcaaaccg gtttcggaac gcctctctcg ggtgagtggc ccggctgtgc
      301 cggacccgag gaggcgagcg ggagtcgaag aggagaagtc ctaggtgggc acagggctgt
      361 cagatggcga gccacgcgat gcgctgcgcg gaggggtgaa tgacccggct gtgccgggcc
      421 atcctccgga ggggaatgat gggcgcgtcc cgctcttgat cgacgatctg ccgccgttct
      481 ggggccgctg ggggcggatc tactggctgg tcgccggcct gctcgccgtc gagaccctcg
      541 tgttctggtg gctgacccgg tgggcgtcgt gaccggcctg gccgcggtcg actgggcggt
      601 gctgggggcg acgatcgccg cgatcgtcgg ctacggcgcg tggcggacgt ttggtgtgcg
      661 tgacgccgac gcctacgtcc gcggcgaggc gctgcgctgg ccgacgatcg gcctgggcat
      721 catggcgacc caggccagcg cgatcacgtt cctgtcgctg ccgggccagg cctacgccga
      781 cggcatgcgg ttcgtgcagt tctacttcgg cctgccgctg gcgacgatcc tgatcagcgc
      841 ggtgttcgtg ccggtctacc accggctccg ggtccgcacc gcctacgagt acctcgagca
      901 ccgcttcgac ctccgggtcc ggctgctcgg cgccctgctg ttcctgatcg gccgcggcct
      961 ggccgccggg atcacgatct acgcgccgtc gatcatcctg tcgcagatcc tgggctggcc
     1021 gctgcactcg acgatctggg gcatcggcgc gctggtcgtg ctctacgtgc tgctcggcgg
     1081 cagccgcgcg gtcagcgtga cccagcgcca gcagatgatc gtgatcctga tcggcctggt
     1141 gatcgccgcg gcgatcgtga tcgcccggct gcccgatcag gtatcgctcg gcggcgcggt
     1201 ccggatcgcc ggcgcgctcg gccggatcaa cccggtcagc ttccagctcg acttcaacag
     1261 ccggtacaac ttctgggccg ggatcaccgg cggcttcttc ctgcagctgt cgtacttcgg
     1321 gaccgaccag tcgcaggtcc agcgctacct gtccggccgc tcggtcgccg agatgcggct
     1381 cggccttttg ttcaacggca tgttcaaggt gccgatgcag ttcctgatcc tgttcatcgg
     1441 cgtgatggtg ttcgtgttct tcctgttcac ccgcccgccg atgcacttcg acgcgcccac
     1501 gctggcccgc gtcgaggccg cccggcccgc cgaggtccac gcgctcgagg cgcggttcga
     1561 tcgcgcgttc gaggcccagc gcggcgcggc gctcgcctac ctcgcggccc ggggcacgcc
     1621 cgacgagccg gccgtccgcg cccggctcgc cacctcggcc gccgagatcg accgggtccg
     1681 cgccgacgcc aagcagctga tccagaccac gctgcccgag gcccagaccc gcgataccga
     1741 ctacgtgttc ctgaccttcg tgctccagta catcccgcac ggcctggtcg gcctgttgct
     1801 cgccgtgatc ctgtgcgcgg cgatgagctc ggtcgccggc gagctgatcg cgctgggctc
     1861 gaccaccgcg gtcgacttct atctccggct ccgcgaggcg ctcggccgac cgccgggttc
     1921 gcggacccgc gacctccgcg tatcccagct cgccatggtg gcgtggggcg tcatcgtgct
     1981 cggcatcgcg agcgcggcct cgctgttcgc caacctgatc gaggcggtca acatcctcgg
     2041 ctcgctgttc tacggcacga tcctcggcct gttcatggtc gcgctgttcc tgcgccggat
     2101 caccgccacc cccgtgctga tcggcgcgat cgtcgccgag ctgctcgtcg ttgcgctgtt
     2161 cttgacatcc acgctgggtt atctgtggtt caacgcgatc ggctgcgcca ccgtcgtggt
     2221 ggtgtcggcg ctgctccaga cggtgctgcc ggcggcctcg tctcctgcct cggctcctgc
     2281 ctcgtcggcc ccgagctccg ggtaggtcgc gacaggggcg acccggcgtg cgatgccggg
     2341 gcgtcgaatc tgcaaactgc tgccatcgcc ccacgggaat cttcagaccg aaacagcgat
     2401 actgccgcgc atatgagagc gctcctcact accctgatcc tgctcggctc cgcgaccgcg
     2461 tacgcgcagc tgaagctccc cgaacccagc cccgccgcaa cggtcgagca gaccatcggc
     2521 atcaccgagg tcaaggtcgt ctaccaccgc cccgagatca agggtcgcac gatctggggc
     2581 cagctcgtgc cgtacaacga gccgtggcgc gccggcgcca acgagaacac cctcgtgagc
     2641 ttctcgtccg acgtcaaggt cggcggcaag ccgctcaagg ccggcaccta cggcctgcac
     2701 atgatcccga ccgcgaagga gtggacgatc gcgttcagca cgatgaccac cgcgtgggga
     2761 agcttcacct acgacccgaa ggaggacgcc ctgcgcgtca ccgtcacgcc gcgcaccgaa
     2821 gcggccagcg aggagcggct ggagtacgac ttcgaggacg tcaccgacgg caaggcgacc
     2881 ctgaggctgc gctgggagaa gctcgcggtg ccgatcgcga tcgaggtcga cacccccaag
     2941 gtcgtgatgg ccagcgtccg cggcgagctg cgcggcctgg ccgggttccg ggccgagacc
     3001 ctgaaccagg ccgcgcagta ctgggtgcag cacggcggca acctcgacga ggcgctcaag
     3061 ttcgtcgaca ccgcgctcca gcgcggcggc cagtaccaga gccacatgct caaggcgcag
     3121 atcctggaga agaagggcaa cgcggcgggc gcggccgacg agcgcaagaa ggccgaggcg
     3181 gtcgccaccg agaatgacct gaaccgcagc gcctacgtgc tgatgaatga caagaaggtc
     3241 gacgaggcga tcaagctgct cgtcgccgcg acccagcgct tccccgaggc gtggaacctg
     3301 caggacagcc tcggcgaggc cctcgcgctc aagggcgaca aggccggcgc gcaggcggcc
     3361 tacagcaagg cgctggcgct cgccaaggac ccggtgcaga agaagcggat cgagggcgcg
     3421 atcgccaagc tgaagtagcg cccgggagtc gcgccgcgcc gcggatcagt tcgcctgcgg
     3481 cgacaccgct tcgaacaccg ggtcctgcga gcgaatgcgg atgaagccca cgctgccacc
     3541 gccaccgcca ccgccgccgt cggcgttcgc cgtcaccgag tcgccggcgg tgccgagcga
     3601 gctgccgccg gcgccgccat cgctgccaag cgcggcg
//
LOCUS       DASVOR010001080         2922 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390511,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001080 DASVOR010000000
VERSION     DASVOR010001080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2922
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390511"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1602
                     /locus_tag="VF516_20715"
     CDS             <1..1602
                     /locus_tag="VF516_20715"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8110171.1"
                     /translation="VATAVFPAMSVAAPPGQAPAGKTAPALGPRMAPDDLPGPQPEVA
                     GESAKVDLPAVPAFALPGVEPGFHGPRELHVQGRHLLGTEIKVKGYITWIYDCASQLA
                     VQHPDKTPQKIATAIQRDPTLCERAKFLLGDAKDTARDASIRVVDVPRPPRRVERGNL
                     KQDQLKGIWPDVPQIAIGDYVIITGTWALVSPHAEHSTDGLLVYKALEHLAPAAAPAA
                     AAPAAIEEPDIDVVTKVPLRKPVSNQVRNASVDHYSACNKANAARQYDAAIAACQAAV
                     QTWPDNHLAWYLWGAAHMAKGEWQKAREAVEHAVTLRPDHAMYQLYYGVALYEAEQER
                     VRDEQARKQARKPEEVEVEPGLLQLDAARDALRRAVKLNPQLWRAHFYLGRVYRDLDD
                     SRRAAEQFAQTITTYPSYRFGYIALTELYRHWSYNDQAAAVAQQGTQHVAPAESGELW
                     FELGMACDARRDDDKAIEAFTHALAIQPGDLVSKFQRGQVYFRKNDFANARRDLEEIA
                     RSSDPSVATTKPLAVQMLDQIARKK"
     gene            1967..>2922
                     /locus_tag="VF516_20720"
     CDS             1967..>2922
                     /locus_tag="VF516_20720"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8110172.1"
                     /translation="MDRHRETFSTCARLLLIGLLASCASLDAADTGSAATAAGADDTP
                     RVAEDHAAFAVTEEGPVRGVATDTTVVFRGIPYAAPPVGDLRWRPPQRVARHRGVLDA
                     TQFANHCPQIAGPFGQGSTTEDCLFLNVFAPNPEHGRGRDRDRDDDRDGDDDGDRSGL
                     RPVMVWIHGGALVTGESNDYDATRLVEQGDVVVVTINYRLGELGFLAHPALTAESPDH
                     ASGNYGLLDQQEALRWVRRNILSFGGDPDRVTIFGESAGGLSVHSQLASPGSAGLFQR
                     AIVQSGAYQLTQPSLATAQAAGTAFAAAAGCTDQTAACLRGLS"
BASE COUNT          459 a         1104 c         1003 g          356 t
ORIGIN      
        1 gttgcgaccg ctgtcttccc cgcgatgtcg gtcgcggcgc cgcccggcca ggcgcccgcc
       61 ggcaagacgg cgccggctct cggcccgcgg atggcgcccg acgacctgcc ggggccgcag
      121 cccgaggtcg ccggcgagag cgccaaggtc gatctgccgg cggtcccggc gttcgcgctg
      181 ccgggcgtcg agcccgggtt ccacggcccg cgcgagctgc acgtccaggg caggcacctg
      241 ctcggcaccg agatcaaggt caagggctac atcacctgga tctacgactg cgcctcgcag
      301 ctcgcggtgc agcaccccga caagacgccc cagaagatcg cgacggcgat ccagcgcgat
      361 ccgacgctgt gcgagcgcgc caagttcctg ctcggcgacg ccaaggacac cgcgcgcgac
      421 gcgtcgatcc gcgtggtcga cgtgccgcgg ccgccgcgca gggtcgagcg cgggaatctc
      481 aagcaggacc agctcaaggg gatctggccc gacgtgcccc agatcgcgat cggcgactac
      541 gtgatcatca ccggcacctg ggcgctggtg tcgccgcacg ccgagcacag caccgacggc
      601 ctgctggtct acaaggcgct ggagcacctc gcaccggccg cggcgccggc cgccgccgcg
      661 ccggccgcga tcgaggagcc cgacatcgac gtcgtcacca aggtgccgct ccgcaagccg
      721 gtcagcaacc aggtccgcaa cgcgtccgtg gaccactaca gcgcctgcaa caaggcgaac
      781 gcggcgcggc agtacgacgc cgcgatcgcc gcatgtcagg ccgcggtcca gacctggccc
      841 gacaaccacc tcgcctggta cctgtggggc gccgcccaca tggccaaggg cgagtggcag
      901 aaggcccggg aggcggtcga gcacgcggtg acgctgcggc ccgatcacgc gatgtaccag
      961 ctgtactacg gcgtcgcgct gtacgaggcg gagcaggagc gggtgcgcga cgagcaggcg
     1021 cgcaagcaag ccaggaagcc cgaggaggtc gaggtcgagc ccgggctgct ccagctcgac
     1081 gccgcgcgcg acgccctgcg ccgggcggtc aagctcaatc cccagctgtg gcgcgcccac
     1141 ttctacctcg ggcgggtcta ccgcgatctc gacgactccc ggcgcgcggc cgagcaattc
     1201 gcccagacga tcacgaccta cccgagctac cggttcggct acatcgcgct gaccgagctg
     1261 taccggcact ggagctacaa cgaccaggcg gcggcggtcg cgcagcaggg cacgcagcac
     1321 gtcgccccgg cggagtccgg cgagctgtgg ttcgagctcg gcatggcgtg cgacgcccgg
     1381 cgcgacgacg acaaggcgat cgaggcgttc acccacgcgc tcgcgatcca gcccggcgac
     1441 ctggtctcga agttccagcg cggccaggtg tacttccgca agaacgactt cgcgaacgcc
     1501 aggcgcgacc tggaggagat cgcccggtcc agcgatccgt cggtcgcgac caccaagccg
     1561 ctcgcggtcc agatgctcga tcagatcgcc cgcaagaagt agattccgca aacggaatcc
     1621 cccgcgggcg cggaccgggc cccgggcaga tcgccacgcg ctcgggcccg cgatgtcgcg
     1681 ccgcgtcgtg atccagcgca ccacgcgacc cacgcgcgac gaggatgcca cgcacggaga
     1741 tcggcaccgc cgcgtcctgg cgattgcgcg ctgccgtaca tgaatgcgcg tcgcggtgag
     1801 ctgagacacg aggtgcctgc gtcgagtggt caaaccacaa cgacagaccg ccgagagatt
     1861 aggaactccc tggttccctt taccgcggat caggcatgat cgaccagctt ctgcgaccca
     1921 gcggcgcgcg ccgctgaccg ccatccacgc gtgacggagg atgaacatgg atcgacaccg
     1981 agagacgttt tcgacgtgcg cccgcctgct cctgatcggg ctcctcgcga gctgcgcctc
     2041 gctcgacgcc gcggacaccg ggtccgccgc caccgccgcc ggggcggatg acaccccgcg
     2101 cgtcgccgag gatcacgccg cgttcgcggt caccgaggaa ggcccggtgc gcggcgtcgc
     2161 gaccgacacc accgtggtgt tccgcggcat cccctacgcc gccccgccgg tcggcgatct
     2221 gcggtggcgg ccgccgcagc gcgtggcgcg gcaccgcggc gtgctcgacg ccacccagtt
     2281 cgccaaccac tgcccgcaga tcgccggccc gttcggccag ggcagcacca ccgaggactg
     2341 cctgttcctc aacgtgttcg cccccaaccc ggagcacggc cgcggccgcg atcgcgaccg
     2401 cgatgacgac cgcgacggcg atgacgacgg cgatcgctcc ggcctgcgcc cggtgatggt
     2461 gtggatccac ggcggcgcgc tggtcaccgg cgagagcaac gattacgacg cgacgcgcct
     2521 ggtcgagcag ggcgacgtgg tcgtcgtcac gatcaactac cggctcggcg agctcggctt
     2581 cctggcccac ccggcgctga ccgccgagtc gcccgaccac gcgtcgggca actacggcct
     2641 gctcgaccag caggaggcgc tgcgctgggt gcgccgcaac atcctgtcgt tcggcggtga
     2701 ccccgatcgc gtgacgatct tcggcgagtc ggccggcggg ctcagcgtcc acagccagct
     2761 cgcgtcgccc ggctcggccg gcctgttcca gcgcgcgatc gtccagagcg gcgcgtacca
     2821 gctgacccag ccgagcctgg ccaccgccca ggcggccggc accgcgttcg cggccgccgc
     2881 gggctgcacc gatcagaccg cggcctgcct gcgcggcctg tc
//
LOCUS       DASVOR010001081         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_11606,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001081 DASVOR010000000
VERSION     DASVOR010001081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11606"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..192
                     /locus_tag="VF516_20725"
     CDS             <1..192
                     /locus_tag="VF516_20725"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8110173.1"
                     /translation="LRLRPALAATEQAYLAAAMARAKGNQTAAARLLGLSRFGLQKKL
                     RRLAGGDAEEEEEEKDGAD"
     gene            complement(202..1083)
                     /locus_tag="VF516_20730"
     CDS             complement(202..1083)
                     /locus_tag="VF516_20730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110174.1"
                     /translation="MLSTLAAIAGCGVVESPMADDAGPACDPLAAFDPPQPLLMTDAP
                     GSSADPALSADELTLYLTRSSPAGDHDLFVATRSRVTDPFGAPVPLAAVDSTSSDAVP
                     TLAAAGLDLVFESDRIAGEGKHLYVATRSSALSEFGAPALVAGVRSPDATDDDQEPFL
                     TADGKELWFISNRTGNNEIFRATWSAGGVASAAVVPALSSSSAEQHVILSADRRTVYF
                     SSNRSAPGARGGFDIYRAHRNSVSDGFGPPAVVSELNTASDDSARWLSADSCRLYMHA
                     KGSSGFTLLVATRHPAM"
     gene            complement(1333..>1975)
                     /locus_tag="VF516_20735"
     CDS             complement(1333..>1975)
                     /locus_tag="VF516_20735"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110175.1"
                     /translation="DAGALGDPALDALDYPWFVKLAHEDASVGITEENLVATPAALRK
                     RAGELIAEHDQAVLAERYVDGRELNVTLIGNADDLQVLPLHEIDFTAMPADRPRIVSY
                     AAKWDESHVDYAGTKPVPLRDASPALTAAVEQVARAAWHALGLREYGRIDLRVDAGGT
                     PWVIDVNPNPDISPDAGVARAAQIAGLSYPQLIARIAEVAHRRIRSMGRRSPG"
BASE COUNT          272 a          711 c          743 g          249 t
ORIGIN      
        1 ctccggctgc ggcccgcact cgccgccacc gagcaggcct acctcgcggc ggcgatggcg
       61 cgcgccaagg gcaaccagac cgcggcggcg cgcctgctcg ggctgtcgcg gttcgggctg
      121 cagaagaagc tgcgccggct cgcgggaggc gacgccgaag aagaagaaga agaaaaagac
      181 ggcgccgact gacccgcgcc gtcacatcgc cgggtggcgg gtcgcgacga gcagggtgaa
      241 accgctgctg cccttggcgt gcatgtacag ccggcagctg tcggccgaca gccagcgcgc
      301 cgagtcgtcg ctcgcggtgt tgagctcgga gaccacggcg ggcgggccga agccgtcgga
      361 caccgagttg cggtgcgcgc ggtagatgtc gaagccgccc ctggcgcccg gcgcggagcg
      421 gttggacgag aagtacaccg tccggcggtc ggcgctcagg atcacgtgct gctcggcgct
      481 cgacgagctc agcgcgggga ccacggcggc gctggcaacg cccccagcgc tccaggtcgc
      541 ccggaagatc tcgttgttgc cggtccggtt ggagatgaac cacagctcct tgccgtccgc
      601 ggtcaggaac ggctcctgat cgtcgtcggt cgcgtccggc gagcgaaccc cggcgaccag
      661 cgccggcgcg ccgaactcgg acagcgccga cgaccgcgtc gccacgtaga ggtgcttgcc
      721 ctcgccggcg atccggtcgg actcgaacac caggtcgagc cccgccgcgg cgagcgtcgg
      781 gacggcgtcg ctgctggtcg agtccacggc cgccagcggg accggcgcgc cgaacgggtc
      841 ggtgacgcgg ctgcgcgtcg ccacgaacag gtcgtgatcg cccgccggcg agctccgcgt
      901 caggtacagc gtgagctcgt cggccgacag cgcggggtcc gcggagctgc cgggcgcgtc
      961 ggtcatcagc agcggctgcg gcggatcgaa cgcggccagc ggatcgcacg cggggccggc
     1021 gtcgtccgcc atcggcgact cgacgacgcc gcacccggcg atcgcggcca gggtcgagag
     1081 cacacacacg gttcggaagg acgggagcct gtggttcgcc atgatgcgcg tccacagtgc
     1141 gatcggaatg ccagcgcggc agcggcccga tcgcgcggtc cggtccatgc ctcgggtcgc
     1201 tgactccgcg aaatcccgag catcggccgg gcacgggccg atccaccgcc gcggacgcgg
     1261 acggccccga gtccgctgca gcggacgtaa gtctcgcggc gcggacggcc gccggtctct
     1321 gcggagctgc acctaccccg gcgagcgccg ccccatcgac cggatccggc gatgcgcgac
     1381 ctcggcgatc cgcgcgatca gctgggggta cgacagcccc gcgatctgcg cggcgcgcgc
     1441 caccccggcg tcgggcgaga tgtcggggtt ggggttgacg tcgatcaccc acggcgtgcc
     1501 gccggcatcg acccgcaggt cgatccggcc atactcgcgc aggccgagcg cgtgccacgc
     1561 cgcgcgcgcc acctgctcga ccgccgcggt cagcgccggg ctggcgtcgc gcagcggcac
     1621 cggcttggtc ccggcgtagt cgacgtggct ctcgtcccac ttggccgcgt agctgacgat
     1681 gcgcggccgg tcggccggca tcgcggtgaa gtcgatctcg tggagcggca ggacctggag
     1741 gtcgtcggcg ttgccgatca gcgtgacgtt gagctcccgg ccgtcgacgt agcgctcggc
     1801 gagcaccgcc tggtcgtgct cggcgatcag ctcgccggcg cgcttgcgca gcgccgccgg
     1861 cgtcgcgacc aggttctcct cggtgatccc gaccgaggcg tcctcgtgcg cgagcttgac
     1921 gaaccacggg tagtcgagcg cgtcgagcgc cggatcgccg agcgcgccgg cgtcg
//
LOCUS       DASVOR010001082         7058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_303264,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001082 DASVOR010000000
VERSION     DASVOR010001082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7058
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303264"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VF516_20740"
     CDS             complement(<1..1173)
                     /locus_tag="VF516_20740"
                     /inference="COORDINATES: protein motif:HMM:NF026115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HEX8110176.1"
                     /translation="MTDKPIRCRLTISHRDFILQNHRVYGTSVMPGVTFADILFRILH
                     ARGFDLSGVVLRDIVFPRPLATAEGLDREVVVTIEGGEVSIVSRPVTQRADDDGWTRH
                     LAARLSFEPPLEDRVLDALAWKAASREVRDMAELYAQARDEAIVHGAPMRCTGQLFLG
                     DGGLLAELELDPSTRAHDGRFHLSPAALDASTLVAFARTPALDAPFIPIALASLRAPV
                     ALRGRFYVHVPAQEQLAPSRDVMHNSYAIHDATGRRVAELTRLTCKRIREPHAITTLV
                     DRADPAPQGDRFTRQLRRMVGRALQVAPERVSTAAGFYELGLDSAAMLQISQELESFV
                     GAPLYPTLLFEHGNIAALARHLEQAHGGGDAAAPAGELTAFRRVWRPASQADGIVQT"
     gene            1412..1855
                     /locus_tag="VF516_20745"
     CDS             1412..1855
                     /locus_tag="VF516_20745"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8110177.1"
                     /translation="MQVKLMHGIFVNKPPAVVFDFLSNPDNMLQWQSSMFEIKGKTKA
                     NAHGKLQHGTKVHDHRKVLGKQIDAEWEVVDLVQDKKLVLQVTSGTVPWKTTYLLEPH
                     EGGTHLAVEGMGDLGQVPVSASAAQRTCQRMFEQDLTTLADILEH"
     gene            2214..2624
                     /locus_tag="VF516_20750"
     CDS             2214..2624
                     /locus_tag="VF516_20750"
                     /inference="COORDINATES: protein motif:HMM:NF039673.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6157 family protein"
                     /protein_id="HEX8110178.1"
                     /translation="MNYIDAFIAVADDSPTAQSLVPQEKGGKKTVAVIQYEMLVDHPY
                     ELTQEDVLFETHARHKEISAAELKKNGKKLRDEFFAKDQPCLRTSPLARKYGWGFHFD
                     PKGKVALYAIESKEYKQMAKKASKVLKALRSSRA"
     gene            2645..2998
                     /locus_tag="VF516_20755"
     CDS             2645..2998
                     /locus_tag="VF516_20755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8110179.1"
                     /translation="MANRISWFEIIGGDAATLHNFYSKLFGWNIDANNAMKYGLVSEA
                     QAGLGGGIGPDPEGGPGYVTFYVGVDDIKAAIAKAEKLGAKVVMPPTAIPEFNVTFAL
                     FSDPAGHVVGLTQNA"
     gene            complement(3044..4225)
                     /locus_tag="VF516_20760"
     CDS             complement(3044..4225)
                     /locus_tag="VF516_20760"
                     /inference="COORDINATES: protein motif:HMM:NF019640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8110180.1"
                     /translation="MTTTNPTSVVEDILDRVLPVLGAHLGEGERLRRLPDATAEALLS
                     SGAFLAWVPRELGGHELPPAEFARMVEAISRVDSGAGFIVGNCNTNAYMMVALPEEGV
                     REIFADRRSVVVGGGFPPAQAVPVPGGYRITGQAGFASGAHLASWVFAFAMIVENGAP
                     RIGPDGMPSMLIGVMRRSECELLDTWHTLGLRSSGSHDFKYTNVFVPSSRTAPLAPLV
                     NPNPLFRGPVYRARLWAGHPAFAVTGLGVARAALDIAFELAQHKTPNFMMQKLGDSPS
                     VQRLLGRAEARWRAARAYIYETVDKLWKHQLTGEFVTHEHAIDMQLASSFAIEAAREV
                     TELVHEIAGSTGFRENSPLERLFRDAHTMSQHAFTSAARFESAAKAMLGRDNDWAFFR
                     L"
     gene            complement(4298..5530)
                     /locus_tag="VF516_20765"
     CDS             complement(4298..5530)
                     /locus_tag="VF516_20765"
                     /inference="COORDINATES: protein motif:HMM:NF020129.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004421 - hydroxymethylglutaryl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0010142 - farnesyl diphosphate biosynthetic
                     process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEX8110181.1"
                     /translation="MIAGIEAIHLYAARAALDVQQLFSARGLDLQRFGNLMMRQKSVN
                     LPCEDPVTNGVNAARPLIAALTPAQRDRIELLIVATESGLDYAKPISTYIHEYLGLSR
                     HCRSFEVKHACYAGTAALQMAASFVAASPVPGLKALVIAAEAASVADRGTYWEPSQGA
                     GAVSMLVSAQPDILALDPGASGYYTYEVMDTLRPRPDLEAGDTDLSLMSYLECLQRTY
                     ALYCERVSGADLLETFDYLVFHPPFAGMVKGAHRTLLRKQRGMSVEAIERDFEARIAP
                     SLVYCSRIGNLYSGSLYAALCSLIDHVANGGDRRIGMYSYGSGCGSEFYSGVVPAGAR
                     DKLAALQIGAAVDARRLLTMDEYELISDLGVERMGGVRDMVFDTARYRAAYDDCLAGR
                     GLLVLDRITNFHRRYRWS"
     gene            complement(5527..5757)
                     /locus_tag="VF516_20770"
     CDS             complement(5527..5757)
                     /locus_tag="VF516_20770"
                     /inference="COORDINATES: protein motif:HMM:NF024851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX8110182.1"
                     /translation="MTGEESRVLEVRTDGNRCCGAGHCVRIAPGVFDQDERDGTVILL
                     ERRPAHDLAPLVHEAAAVCPTRAITVTVQVRR"
     gene            complement(5747..6196)
                     /locus_tag="VF516_20775"
     CDS             complement(5747..6196)
                     /locus_tag="VF516_20775"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110183.1"
                     /translation="MTDDAKGETMLQHLGGRDRLRAFVEHFHHSVLSDDLLGELFANA
                     RPTHSAHLLAFFEEVMGGRKVYTERHNGVEGLFDAHANLEITDAQRRRFVALMLEAAD
                     SVGLPADERFRSALARRIEAGSMFSMTLSQVGAERLWPWPPVGTYDW"
     gene            complement(6193..>7058)
                     /locus_tag="VF516_20780"
     CDS             complement(6193..>7058)
                     /locus_tag="VF516_20780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8110184.1"
                     /translation="RYAAIGRGEPAVVILNAFGQTVAPWLPLIERLSRHRRVLLWEPR
                     EAGAGGTPVTFVEHCEDLAAILAQERARPCHLVGWCTGAKLAARYCRMDPGAVASQVM
                     LGGSFKHAGRAPELDTPYERNLEAVLQAVAHEPALAERLRRVLVRTADGEVDLDRLDG
                     HALAHHAVTRVPDGLKQEIRQPFRDAASLRVYARQHLDFWSFDETASAPHTPVPTLGV
                     TGQHDQIVSPAGFRAAVERFPVSRFHAVAATTHHCFHERPDAVAELIESWIGLHNSAQ
                     RHPAPHGGIDA"
BASE COUNT         1190 a         2439 c         2410 g         1019 t
ORIGIN      
        1 ggtctgcacg atgccgtccg cctgcgatgc ggggcgccac acgcggcgga acgcggtgag
       61 ctcgcccgcc ggcgccgccg cgtcgccccc gccgtgggcc tgctcgagat gccgcgcgag
      121 cgccgcgatg ttgccgtgct cgaacagcag ggtcgggtac agcggcgcac cgacgaacga
      181 ttcgagctcc tggctgatct gcagcatcgc cgcggagtcg agccccagct cgtagaagcc
      241 ggccgccgtg ctgacccgct cgggagcgac ctgcaaggcg cgaccgacca tgcggcgcag
      301 ctggcgcgtg aagcgatcgc cctgcggcgc ggggtccgcg cggtcgacca gggtggtgat
      361 cgcgtgcggc tcgcggatcc gcttgcaggt caggcgcgtg agctcggcga cgcgccggcc
      421 ggtggcgtcg tggatcgcgt agctgttgtg catcacgtcg cgcgacggcg cgagctgctc
      481 ctgggccggt acgtggacgt agaaccgccc gcgcagcgcg accggcgcgc gcaacgacgc
      541 gagcgcgatc gggatgaacg gtgcatccag ggccggggtc cgcgcgaacg cgaccagggt
      601 ggaggcgtcg agcgcggcgg gcgagagatg gaacctgccg tcgtgggccc gggtcgaggg
      661 atcgagctcg agctcggcga gcaggccgcc gtcgccgagg aacagctggc cggtgcaccg
      721 catcggcgcg ccgtggacga tcgcctcgtc gcgcgcctgc gcgtagagct ccgccatgtc
      781 ccgcacctcg cgcgacgccg ccttccaggc aagggcgtcg aggacacggt cctcgagcgg
      841 gggctcgaag ctcagcctgg ccgccaggtg ccgggtccag ccgtcgtcgt ccgcgcgctg
      901 cgtgaccggg cggctgacga tcgagacctc gccgccctcg atcgtcacca cgacctcgcg
      961 atcgagacct tcggcggtcg cgagcggacg ggggaacacg atgtcgcgga gcacgacccc
     1021 cgacaggtcg aacccgcggg cgtggagaat gcgaaacagg atgtcggcga aggtcacacc
     1081 gggcatcacg ctcgtgccgt acacccggtg gttctgcagg atgaagtcac ggtgcgagat
     1141 cgtcagccgg catcggatgg gcttgtctgt cacggcagcg gacctcgcga tcgagtgaac
     1201 agacccaaca ggtgccaccg catcacgcgg gtcgctgctc acaactgagc gccgccgggc
     1261 tcgcggcgag caggggccaa aaactcgctc ggcctgctca caattgagca gctcccggcc
     1321 tgcgcgcgag tacataacca ccagggctac aactgtagca ttgtactgat cctcaacatt
     1381 cttgtcccat catcgaatcg gaggctgagt tatgcaagtc aaactgatgc acggcatctt
     1441 tgtcaacaag ccgcccgcgg tagtcttcga cttcctctcc aatcctgaca atatgctgca
     1501 atggcagtcc tcgatgttcg agatcaaggg caagaccaag gcgaacgccc acggcaagct
     1561 gcagcacggc accaaggttc acgatcaccg caaggtcctg ggcaagcaga tcgatgccga
     1621 atgggaggtc gtcgatctcg tccaggacaa gaagctggtc ctccaggtca cgtccggcac
     1681 ggtgccctgg aagaccacgt acctgctgga gccgcacgag ggcggtaccc acctcgccgt
     1741 ggaaggcatg ggggatctcg gtcaggtgcc ggtgtccgcc agcgccgcgc agcgcacgtg
     1801 ccagcgcatg ttcgagcagg acctcaccac gctcgcggac atcctcgagc actgaccaca
     1861 acacgccggc cgcgcggtca ccgcgcatcc agcacggtgc cgcctcccgg cggacccgcg
     1921 cgcccggcgc tgcgacccgc tgacgtcacc gcgcgcaggc gcgcaggcgc gcagcgatcc
     1981 ggaggaacac cggcgccccc caccgctgga ggagatctcg gtgcatcgca gctcacgatc
     2041 ggcgtctgcc ggaggcatcg ctgcgcacac cgcgatcgcc gcatcctccg gtccggcgtt
     2101 ccggatgatg cgggtgacgt gatgaacgat cgagggtagg atgccgctcg gcaggatcgc
     2161 tgccggccca gcacccaccg tcagctccga gactgaactg gaggcctcac ccgatgaact
     2221 acatcgatgc tttcattgcc gtcgccgatg actctccgac cgcccagagc cttgttcccc
     2281 aggagaaggg cggcaagaag acagtcgccg tgatccagta cgagatgctc gtcgatcacc
     2341 cgtacgagct cacgcaggag gacgtcctgt tcgagaccca cgcgcgacac aaggagatct
     2401 cggcggcgga gctcaagaag aacggcaaga agctgcgcga cgagttcttc gccaaggatc
     2461 agccttgcct ccgcacctca ccgctggcgc gcaagtacgg ctggggcttt catttcgatc
     2521 cgaagggcaa ggtggcactc tatgctatcg aatcgaaaga gtacaaacag atggccaaga
     2581 aggcctcgaa ggtgctaaag gccttgcgat cgagccgcgc ctagcgcggg gaaagaatac
     2641 cacaatggca aaccggatca gctggttcga gatcatcggc ggtgatgctg ccacgcttca
     2701 taacttctac tccaagctgt tcggctggaa catcgacgcc aacaacgcca tgaaatacgg
     2761 gctggtctcc gaggcgcagg ccggtctcgg cggcggcatc ggccccgacc ccgaaggcgg
     2821 acccggctac gtgacgttct atgtcggggt cgatgacatc aaggcggcga tcgccaaggc
     2881 cgagaagctc ggcgccaagg tcgtgatgcc gcccaccgcg atccccgagt tcaacgtcac
     2941 cttcgcgctg ttctcggatc cggcgggtca cgtggtcggg ctgacccaga atgcctgaac
     3001 cggccatccc cgaccgctcg gggaggtcct gcgccgacag cacctacagc cggaagaacg
     3061 cccagtcgtt gtcgcgcccg agcatggcct tggccgcgga ctcgaagcga gccgccgagg
     3121 tgaacgcgtg ctggctcatg gtatgcgcgt cccgaaacag ccgctcgagc gggctgttct
     3181 cgcggaatcc ggtcgagccc gcgatctcgt gcacgagctc ggtcacctcg cgggcggcct
     3241 cgatggcgaa cgacgaggcg agctgcatgt cgatggcgtg ctcgtgcgtg acgaactcgc
     3301 cggtcagctg gtgcttccac agcttgtcga cggtctcgta gatgtacgcg cgagccgcgc
     3361 gccacctggc ctctgcgcgg cccagcaggc gctggacgga tggactgtct cccagcttct
     3421 gcatcatgaa gttgggggtc ttgtgctgcg cgagctcgaa ggcgatgtcg agcgccgccc
     3481 gggcgacgcc gaggccggtc accgcgaacg ccggatggcc agcccacagc cgggcgcgat
     3541 acaccggccc ccggaacagc ggattgggat tgaccagcgg tgccagcggt gcggtgcggc
     3601 tgctcggcac gaacacgttc gtgtacttga agtcgtggct gccgctgctg cgcaggccca
     3661 gcgtgtgcca ggtatcgagc agctcacact cgctgcggcg catcaccccg atcagcatgc
     3721 tgggcatccc gtcggggccg atccgcggcg ctccgttctc cacgatcatc gcgaacgcga
     3781 acacccacga cgcgaggtgc gcgcccgacg cgaagccggc ctgcccggtg atgcggtagc
     3841 caccggggac cggcaccgcc tgcgccggcg gaaatccgcc accgaccacg accgagcggc
     3901 gatcggcgaa gatctcgcgg acgccctcct cggggagcgc gaccatcatg tacgcgttgg
     3961 tgttgcagtt gccgacgatg aacccggcgc ccgaatcgac cctcgagatc gcctcgacca
     4021 tgcgcgcgaa ctcggccggc ggcagctcgt gtccgccgag ctcgcgcggg acccaggcaa
     4081 ggaacgctcc cgaggagagg agcgcctcgg cggtcgcatc ggggagccgg cggaggcgct
     4141 cgccttctcc caggtgcgcg ccgagcacgg ggagaactcg gtcgaggatg tcctcgacga
     4201 cgctggtcgg attcgtggtg gtcatgtttc gcatgctagg gcagcacgcc gccaccgact
     4261 gctcataatt gagcgcaggg cggaacgcca ctcggcatca cgaccagcga tatctgcgat
     4321 ggaagttcgt gatccggtcg aggacgagca agccgcgacc ggccaggcag tcgtcgtagg
     4381 cggcgcgata gcgcgcggtg tcgaacacca tgtcccgcac gccacccatg cgctcgacgc
     4441 cgaggtcgct gatcagctcg tactcgtcca tcgtgagcag ccgcctggca tcgaccgcgg
     4501 cgccgatctg cagcgcggcc agcttgtcgc gtgcaccggc cgggacgacg ccgctgtaga
     4561 actcggagcc gcagcccgag ccgtacgagt acatcccgat ccgccggtcc ccaccgttcg
     4621 ccacgtgatc gatcagcgaa cacagcgccg catacagcga tccggaatac agattgccga
     4681 tccggctaca atacaccagc gagggcgcga tccgggcctc gaagtcgcgc tcgatcgcct
     4741 cgacgctcat cccgcgctgc tttcgaagca gcgtccggtg ggcgcccttc accatcccgg
     4801 cgaacggggg atggaacacg agataatcga aggtctcgag gagatcggcg cccgacacgc
     4861 gctcgcaata cagcgcatag gtccgctgca ggcactcgag gtacgacatc agcgacaggt
     4921 cggtgtcgcc ggcctccaga tcgggacgcg ggcgcagggt gtccatcacc tcgtaggtgt
     4981 agtaaccgct cgcgcccgga tcgagcgcca ggatgtcggg ctgtgcactg accagcatgc
     5041 tcaccgcgcc ggcgccctgc gatggctccc agtaggtccc gcggtccgcg acgctggccg
     5101 cctccgcggc gatcaccagc gccttcagcc cgggcaccgg actcgccgcc acgaagctgg
     5161 ccgccatctg cagcgccgcg gtcccggcgt agcacgcgtg cttgacctcg aacgagcggc
     5221 agtggcggct caggccgagg tactcgtgga tgtacgtgct gatcggcttg gcgtaatcca
     5281 gtccggactc ggtggcgacg atcagcagct cgatccgatc gcgctgcgcg ggcgtgagcg
     5341 cggcgatcag cggccgcgcg gcgttgaccc cgttggtcac cgggtcctcg cacggcaggt
     5401 tgaccgactt ctggcgcatc atcaggttcc cgaaccgctg gaggtcgagc ccgcgcgcgc
     5461 tgaacagctg ctgcacgtcg agcgccgcgc gcgcggcgta gaggtggatc gcctcgatgc
     5521 ccgcgatcat cgcctgacct gcaccgtcac cgtgatcgcg cgggtcggac acaccgccgc
     5581 ggcctcgtgg accagcggcg cgaggtcgtg cgccgggcgg cgctcgagca ggatcacggt
     5641 gccatcgcgc tcgtcctgat cgaacacgcc cggcgcgatc cgcacgcagt gcccggcgcc
     5701 gcagcagcgg ttgccgtcgg ttcgcacctc gaggacgcgg gactcctcac cagtcatagg
     5761 tccccaccgg cggccacggc cacagccgct ccgcgccgac ctgcgacagc gtcatcgaga
     5821 acatcgagcc ggcctcgatc cgcctggcga gcgcggagcg gaaccgctcg tcggccggca
     5881 agccgaccga atcggccgcc tccagcatga gggcgacgaa ccggcggcgt tgcgcgtccg
     5941 tgatctcgag gttggcgtgg gcgtcgaaca ggccttcgac gccgttgtga cgctccgtgt
     6001 agaccttccg cccccccatg acctcctcga agaacgcgag caggtgagcg gagtgggtcg
     6061 gcctggcgtt cgcaaacagc tccccgagca gatcgtcgga cagcacgctg tggtggaagt
     6121 gctccacgaa cgcacgcagc cgatcccgcc cgccgaggtg ctggagcatg gtctcgccct
     6181 tcgcgtcgtc ggtcatgcgt cgatcccacc atgcggcgcg gggtggcgtt gcgcactatt
     6241 gtgcagtccg atccaggact cgatcagctc ggccaccgca tcgggccgct cgtggaaaca
     6301 gtggtgggtg gtggcagcga ccgcatggaa gcgggatacc ggaaaccgct cgaccgcggc
     6361 ccggaacccg gccggcgaca cgatctggtc gtgctggccg gtcacgccga gggtgggcac
     6421 cggggtgtgc ggcgcgctcg cggtctcgtc gaacgaccag aagtcgaggt gctggcgggc
     6481 gtacacccgc aagctcgcag cgtcgcgaaa cggctggcgg atctcttgct tgaggccgtc
     6541 cggaacccgg gtgaccgcgt ggtgggcgag ggcatggccg tccagccgat cgaggtcgac
     6601 ctctccgtcc gcggtccgga ccagcacccg ccgcaagcgc tcggccagcg cgggctcgtg
     6661 cgcgaccgcc tgcaggaccg cttcgaggtt gcgctcgtac ggcgtatcga gctcgggggc
     6721 gcgcccggcg tgcttgaacg agccgccgag catcacctgc gaggccaccg cgccggggtc
     6781 catccggcag taccgcgccg cgagcttggc gccggtgcac cagcccacca ggtgacaggg
     6841 ccgtgcccgc tcctgcgcga ggatcgcggc gaggtcctcg cagtgctcga cgaacgtgac
     6901 cggcgtgccc ccggcgccgg cttcgcgcgg ctcccacagc aacacgcggc ggtggcgcga
     6961 cagccgctcg atcaacggca gccacggcgc gacggtctgc ccgaaagcgt tgaggatgac
     7021 caccgccggc tcgccgcggc cgatcgcggc gtagcgca
//
LOCUS       DASVOR010001083         3849 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_273273,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001083 DASVOR010000000
VERSION     DASVOR010001083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273273"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(360..1370)
                     /locus_tag="VF516_20785"
     CDS             complement(360..1370)
                     /locus_tag="VF516_20785"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX8110185.1"
                     /translation="MRTIAAAGLVLGLSTPALAQNVVRYDPKPADLKYTFGVARPVAT
                     VKRGDIIDTRTFDCFGNAIQKPGDTLAKVKGDNPLTGPFYVEGAEPGDTLAVKILELT
                     VDSDQGIGALGPGFGALNSTSYTPMLNPPVPEKIWFYVIDRARNEATFSALDSKFQTR
                     IPLHPFLGCLGVAPALEARSSVVPAEHGGNMDAPEASAGYTAYFPVNVRGALLYLGDG
                     HAQMGDGEIAGTAIEVPMRARLQVDVIKGQKIAWPRFENSREIMALGAYRPLDDALRI
                     AFTELIAWIHHETGLSELDAYQLLSQVGKIHVTEMVDPNYVVIASVDKKYLPPRQNGK
                     PW"
     gene            complement(1415..3577)
                     /locus_tag="VF516_20790"
     CDS             complement(1415..3577)
                     /locus_tag="VF516_20790"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8110186.1"
                     /translation="MALPAGGCADDVQWSADGKRFAFRNTSSTAVELWLGEAATGAVR
                     RLGDARLNPMLGSSYQWLADQRTLLVKLVPDHAGPPPAASGATGGPSIQETHGESGES
                     STYETRDTLTSKHDEDLFEYYAASQLARIDAATGAVTPIGKPDAFTDVEAAPDGDHLL
                     VTVIRKPYSYVTTYERFAHDVEVRDRTGHVTRALAHLPVADRVPIHGVPSGPREFAWR
                     RTEPATLVWAEALDKGDWNVTVPARDKVMMQSAPFTAPAIEIARTEQRYDGLEWSEQR
                     RVSLLHEIDLNRHWQRTFVVNVDDPRARPRVLWDLSSDEQYKDPGRPLVRVLPGGQWV
                     IRLDGDAIYLQGWGASPQGDRPFLDRLDLATGKTQRLFRSDRQALERVLDFTGGTTRS
                     FLTWHESPTDPPNAMLRTLGDALPPASVAPGEAGVASTVRAVTHVPDPTPVVRAIRKR
                     LVKYRRKDGTELSFTLYTPPGYREGTRIPAILYAYPLDYASASTAGQVSGSDQRFTLL
                     YNYQLLLLSGYAIIDNAAFPIVGDPKQAYDTYLEQLVDDARAAVDKAVELGVVDRDRI
                     GVTGHSHGALMTVNLIAHTDLFRAGVATSGSYNKTLTPFGFQNERRTVWKAQPVYLKV
                     SPFFFADKIKHPLLIMHGMEDANPGTTPLQAVKLYEAIRGNGGTARLVMLPHEPHWYS
                     AMESNEQLAYEELRWFDRYVKSAPPRGHSSDAGTPAAP"
     gene            complement(3584..>3849)
                     /locus_tag="VF516_20795"
     CDS             complement(3584..>3849)
                     /locus_tag="VF516_20795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110187.1"
                     /translation="YDRPPKHVLDILHAASPPEPMPSPTRDTLLLVSWVDYPPIAQVA
                     EPYLRLAGVRLEPRTRRKHDTPAGYGIAPCARSFTLVDVATRR"
BASE COUNT          522 a         1237 c         1464 g          626 t
ORIGIN      
        1 gctgtgctgt ggcgtggtcg cgccacgggc gccgcggtcg gtgccggccg ggcttctgat
       61 gcggaggctc taccggggat cccggttgac tcgacccgcg ccgcatgggg tacggtgcaa
      121 gcaggcccaa tcactttgtg aataggaacc agatccaggt cgctatagca taaactgaag
      181 caatttccgg ttgttaagcg tagatatcac gccggtgatt tgaacctatt cgtcgccagc
      241 tcagagtgcg ccggtcgagg tctagagcgt gctcggtggg cttcacgtcg actgccgcag
      301 ggccgcttga cgcctggcga ttctgccgga cgacatcggt agcgagtatg gggtggcgct
      361 caccagggct tgccgttctg gcggggcggc aggtacttct tgtcgaccga ggcgatcacg
      421 acgtagttcg gatcgaccat ctcggtgacg tggatcttgc cgacctgcga cagcagctgg
      481 taggcgtcga gctccgagag gccggtctcg tggtggatcc aggcgatcag ctcggtgaac
      541 gcgatccgca gcgcgtcgtc gagcgggcgg taggcgccga gcgccatgat ctcgcggctg
      601 ttctcgaacc ggggccacgc gatcttctgg cccttgatga cgtcgacctg caggcgggcg
      661 cgcatcggga cctcgatcgc ggtgccggcg atctcgccgt cgcccatctg ggcgtggccg
      721 tcgcccaggt agagcagggc gccgcgcacg ttgaccggga agtaggcggt gtagccggcg
      781 ctggcctccg gcgcgtccat gttgccgccg tgctcggcgg ggaccaccga gctgcgcgcc
      841 tcgagggcgg gcgcgacgcc gaggcagccg aggaacggat gcagcgggat ccgggtctgg
      901 aacttcgagt cgagcgcgct gaacgtggcc tcgttcctgg cgcggtcgat cacgtagaac
      961 cagatcttct ccggcaccgg agggttcagc atcggggtgt agctggtcga gttcagcgca
     1021 ccgaagccgg ggccgagcgc gccgatgccc tggtcgctgt cgaccgtgag ctcgaggatc
     1081 ttgacggcca gggtgtcgcc gggttcggcg ccctcgacgt agaacgggcc ggtcagcggg
     1141 ttgtcgccct tgaccttggc gagggtgtcc cccggcttct ggatcgcgtt gccgaagcag
     1201 tcgaaggtcc gggtgtcgat gatgtcgccg cgcttgacgg tcgccaccgg cctggcgacg
     1261 ccgaacgtgt acttgaggtc ggccggcttg ggatcgtagc ggacgacgtt ctgggccagc
     1321 gcaggggtgg acaggccgag cacgagcccg gcggcggcga tggttcgcat gcgcggagtc
     1381 taacgcagga cgggcggcgg ctcgcgcggg gcgatcacgg cgcggcgggg gtcccggcgt
     1441 cggacgagtg tccgcgcggc ggcgcgctct tgacgtagcg gtcgaaccag cgcagctcct
     1501 cgtaggcgag ctgttcgttc gactccatcg ccgagtacca gtgcggctcg tgcggcagca
     1561 tcaccaggcg cgccgtgccg ccgttgccgc ggatcgcctc gtagagcttg accgcctgga
     1621 gcggcgtggt cccgggattg gcgtcctcca tgccgtgcat gatcagcagc gggtgcttga
     1681 tcttgtcggc gaagaagaac ggcgagacct tgaggtatac cggctgggcc ttccacaccg
     1741 tgcgccgctc gttctggaac ccgaacgggg tgagcgtctt gttgtaggac ccgctggtcg
     1801 cgacgccggc gcggaacaga tcggtgtggg cgatcaggtt cacggtcatc agcgcgccgt
     1861 ggctgtgccc ggtgacgccg atgcgatcgc ggtcgaccac gccgagctcg accgccttgt
     1921 cgaccgctgc gcgggcgtcg tcgaccagct gctcgaggta ggtgtcgtag gcctgtttcg
     1981 gatcaccgac gatcggaaac gcggcattgt cgatgatcgc gtatccggac agcagcaaca
     2041 gctggtagtt gtagagcagc gtgaagcgct gatccgagcc gctgacctgt cccgcggtgg
     2101 acgcgctggc atagtccagc gggtaggcat agaggatcgc cgggatccgg gttccctccc
     2161 ggtagcccgg cggggtgtac agcgtgaacg acagctccgt gccgtccttg cgccggtact
     2221 tgaccagccg cttccggatc gcgcgcacga ccggcgtcgg atcggggacg tgggtcacgg
     2281 cgcggacggt cgacgccacg ccggcctcgc cgggcgcgac cgacgcgggg ggcagcgcgt
     2341 cgcccagcgt gcgcagcatc gcgttgggcg gatcggtcgg cgactcgtgc caggtcaaaa
     2401 agctccgggt cgtcccgccg gtgaagtcga ggacgcgctc cagcgcctgc cggtcgctgc
     2461 ggaacagccg ctgggtcttg ccggtggcga gatcgagccg atcgaggaac ggccggtcgc
     2521 cctgcgggga cgcgccccag ccctggaggt agatcgcgtc gccgtcgagg cgaatcaccc
     2581 actggccgcc gggcaggacg cggaccagcg ggcgcccggg atccttgtac tgctcgtcgc
     2641 tcgacaggtc ccacagcacg cgcggccggg cgcgcgggtc atcgacgttg accacgaacg
     2701 tgcgctgcca gtggcggttg aggtcgatct cgtggagcag gctgacgcgg cgctgctcgc
     2761 tccactcgag gccgtcatag cgctgctcgg tgcgcgcgat ctcgatggcg ggggccgtga
     2821 acggcgcgct ctgcatcatc accttgtcgc gcgccgggac ggtgacgttc cagtcgccct
     2881 tgtcgagcgc ctcggcccag accagcgtgg cgggctcggt ccgccgccac gcgaactcgc
     2941 gcgggccgga gggcacgccg tggatcggca cgcgatcggc gaccggcagg tgcgcgagcg
     3001 cccgggtgac gtggccggtg cggtcccgga cctcgacgtc gtgcgcgaac cgctcgtagg
     3061 tggtgacgta cgagtacggc ttgcggatca cggtcaccag gaggtggtcg ccgtcgggcg
     3121 cggcctcgac gtcggtgaac gcgtcgggct tgccgatcgg cgtgaccgcc ccggtcgcgg
     3181 cgtcgatccg cgcgagctgg ctcgccgcgt agtactcgaa cagatcctcg tcgtgcttgc
     3241 tggtcagcgt gtcgcgggtc tcgtacgtgc tgctctcgcc ggactcgccg tgggtctcct
     3301 ggatgctcgg gcccccggtg gcgcccgacg cggccggcgg cggcccggcg tggtcgggga
     3361 cgagcttgac cagcagggtg cgctggtcgg ccagccactg gtaggagctg ccgagcatcg
     3421 gattgagccg ggcgtcgccc aggcggcgca ccgcgccggt cgcggcctcg ccgagccaga
     3481 gctcgacggc ggtgctcgac gtgttgcgga acgcgaaccg cttgccgtcg gccgaccact
     3541 ggacgtcgtc ggcacagccc ccggcgggca gcgccaccgg ggtctaccgg cgggtcgcga
     3601 cgtcgaccag ggtgaagctg cgcgcgcacg gcgcgatgcc gtagccggcc ggcgtgtcgt
     3661 gcttgcgccg ggtcctgggc tcgaggcgga cgccggcgag tcgcaggtac ggctcggcga
     3721 cctgcgcgat cggcgggtag tcgacccacg acaccaggag gagggtgtcg cgcgtcgggc
     3781 tgggcatcgg ctccggcggc gacgccgcgt gcaggatgtc gaggacatgc ttcggcggcc
     3841 ggtcgtagc
//
LOCUS       DASVOR010001084         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390557,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001084 DASVOR010000000
VERSION     DASVOR010001084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390557"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1472)
                     /locus_tag="VF516_20800"
     CDS             complement(<1..1472)
                     /locus_tag="VF516_20800"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110188.1"
                     /translation="MIRPADLARGAPDAADALRRAERLFDAAAQSAVDLAGLGDLAQR
                     NATLCCQSAPYLATLLTRDPHRLGRVASDPYLRREKPREIVVTEVTACAAPARTPAEL
                     QAALRSVRADELVRLGVRELELGLDTEVGRELARLADACFDAAIGFWDRALRARYGAP
                     RYVDDDGAERDAHLAVIGMGKLGGEELNFSSDVDVIYVYSSDSGEVGPLSLHEYFAKL
                     CTHVTAALSEVTEDDTVFRVDLRLRPEGARGAIANSLAQAERYYETFGRPWERQAWLK
                     ARVSAGAPALGAELLRTLRPFVFPRLTSATVIDDVRELNRRIKRELVRPHQHSGFDVK
                     NGEGGIREIEFFVQALQLIHAGKRPGLRVRGTLAALDALLFAGLIADHEHLALWRAYR
                     WLRHIEHVLQLEGGLQTQIVPDDDARRLVLARRLGYASDVGFAIDLVKHTAAVSRLFA
                     TLGDPGDGRAEVTAILRGELTEDEEADALARLGFHDIAAAR"
     gene            1536..>2045
                     /locus_tag="VF516_20805"
     CDS             1536..>2045
                     /locus_tag="VF516_20805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX8110189.1"
                     /translation="MRDLYEVLEVDRQASPSELKKAYYRLAKKYHPDHNPGDKDAEEK
                     FKEASNAYSILSDDDQRQRYDRFGFEGIRSVNGHAGGYTNVEDILSAFGDIFGDFFGG
                     RGAGRRQPRGADLRVDRTLSFAEAVWGITKDVKVTREIACVTCGGNGARPGSKPEICR
                     TCQGKGQVVH"
BASE COUNT          307 a          755 c          710 g          273 t
ORIGIN      
        1 cgcgccgccg cgatgtcgtg gaacccgagc cgggccagcg cgtcggcctc ctcgtcctcg
       61 gtcagctcgc cgcgcaggat cgcggtgacc tcggcgcgcc cgtcgccggg atcgccgagc
      121 gtcgcgaaca gccgcgacac cgccgcggtg tgcttgacca ggtcgatcgc gaacccgacg
      181 tcgctcgcgt agccgagccg ccgggcgagc accagccggc gcgcgtcgtc gtcgggcacg
      241 atctgggtct gcagcccgcc ctcgagctgc agcacatgct cgatgtggcg cagccagcgg
      301 tacgcccgcc acagcgcgag gtgctcgtgg tcggcgatca gcccggcgaa cagcagcgcg
      361 tcgagcgccg ccagcgtgcc gcgcacccgc agcccgggcc gcttgccggc gtggatcagc
      421 tggagcgcct ggacgaagaa ctcgatctcg cggatcccgc cctcgccgtt cttgacgtcg
      481 aacccgctat gctgatgcgg tcgcaccagc tcgcgcttga tccggcggtt gagctcgcgg
      541 acgtcgtcga tcaccgtcgc cgaggtcagc cgcgggaaca cgaacggccg cagcgtgcgg
      601 agaagctccg cgccgagcgc gggggcgccg gcgctgaccc gggccttgag ccaggcctgg
      661 cgctcccacg gccgcccgaa cgtctcgtag tagcgctcgg cctgcgccag cgagttggcg
      721 atcgcgccgc gcgcgccctc gggccgcagc cggagatcga cgcggaacac cgtgtcgtcc
      781 tcggtgacct ccgacagcgc cgcggtgacg tgggtgcaca gcttggcgaa gtactcgtgg
      841 agcgacagcg gcccgacctc gccgctgtcc gacgagtaga cgtagatcac gtcgacgtcg
      901 gacgagaagt tgagctcctc gccgccgagc ttgcccatcc cgatcaccgc gaggtgcgcg
      961 tcgcgctcgg cgccgtcgtc gtcgacgtag cgcggcgccc cgtagcgcgc ccgcagcgcg
     1021 cggtcccaga acccgatcgc ggcgtcgaag caggcgtcgg ccagccgggc cagctcgcgg
     1081 ccgacctcgg tgtcgagccc gagctcgagc tcgcggaccc cgagccggac cagctcgtcc
     1141 gcccggacgc tccggagtgc ggcctgcagc tcggccgggg tccgggccgg cgcggcgcac
     1201 gctgtgacct cggtcacgac gatctcgcgc ggcttttccc ggcgcaggta tggatcggac
     1261 gctaccctcc ccagacggtg gggatcccgg gtgagcagcg tggcaaggta gggggcggac
     1321 tggcagcaga gtgtggcgtt ccgctgcgcc agatcgccca acccagcgag atccaccgcg
     1381 ctttgcgccg cggcatcgaa cagccgctcg gcgcggcgga gcgcgtccgc ggcatcgggg
     1441 gcgccgcgcg ccaggtccgc cggccggatc acggcctgac cataccttga cacctggcac
     1501 atcccgaatt acctaaggtc ctgattctcc acgccatgcg cgatctctac gaagtccttg
     1561 aagtcgatcg gcaggcgagc ccttccgagc tcaagaaggc gtactaccgc ctcgccaaga
     1621 agtaccaccc ggatcacaac ccgggggaca aggacgccga ggagaagttc aaggaagcct
     1681 ccaacgccta ctcgatcctc tcggacgacg accagcgcca gcgctacgat cggttcgggt
     1741 tcgagggcat ccgcagcgtc aacggccacg ccggcgggta caccaacgtc gaggacatcc
     1801 tgtcggcgtt cggcgacatc ttcggcgact tcttcggagg ccgcggcgcc ggccgccgcc
     1861 agccgcgcgg cgccgatctg cgcgtcgatc ggaccctgtc ctttgccgag gcggtgtggg
     1921 gcatcaccaa ggacgtcaag gtcacccgcg agatcgcctg cgtgacctgc ggcggcaacg
     1981 gcgcccgccc gggcagcaag cccgagatct gccggacctg ccagggcaag ggccaggtcg
     2041 tccac
//
LOCUS       DASVOR010001085         7384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_566548,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001085 DASVOR010000000
VERSION     DASVOR010001085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7384
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566548"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="VF516_20810"
     CDS             complement(<1..258)
                     /locus_tag="VF516_20810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8110190.1"
                     /translation="MNEKTQDLHGLPGERAGGGATAWCGLDEAAATVADGAWISPGGF
                     MLGRAPIALVLALIRARRRDLRILSLPNPLPAELLVAAGAAA"
     gene            383..595
                     /locus_tag="VF516_20815"
     CDS             383..595
                     /locus_tag="VF516_20815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEX8110191.1"
                     /translation="MAGQRGGQGNELARCEQTRQRVLRGDAATLAVMVMTGRGGDPDP
                     ERAEQLFERAEEQGVDVEAFRNQLGL"
     gene            604..1599
                     /locus_tag="VF516_20820"
     CDS             604..1599
                     /locus_tag="VF516_20820"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8110192.1"
                     /translation="MLARLFRDLAPFQPTLIGTYPLGLAVDGSDLDIACACDDLDRFE
                     HALRATLAALGVAARFERSETAVVAAFELGDVPVEIFGQALPVTAQRGFRHMVIEGQL
                     LVIGGAALAARIRDLKRAGTKTEPAFAQVLGLAGDPYEALLALEAWPPDRLRDLVARS
                     LRPAVPPWIGAYSGDRAALVPLFRIADDSDQAIASYLARGTVLVATDGGAPVGHVQMI
                     EPGRGPGSTPGNAIDEPATWELTSLAVVDSHRGHGLGRRLVRAGIAYARSHGASRVVL
                     ATAAADTTVLQFYQRIGFRLLRIERDAFTPAAGYPPELCVDGIPLLDRVWLDFTW"
     gene            complement(1731..2648)
                     /locus_tag="VF516_20825"
     CDS             complement(1731..2648)
                     /locus_tag="VF516_20825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110193.1"
                     /translation="MPTNSRFAAVRDHVAPRPPPDAAVDRVIYGLVQPLVGARTLAAH
                     RDLLRAALVPAGLLAACCAVVAALGDRGSFLHRFYTTFAVLAPLPSIVLADHYARLAA
                     QARSVLGFGPVEPCIEPLRRNLGRAAKQAILIAIALAPVTGLLHLVPGTGWLLVQVVA
                     AVWALHWVVVEAFDSARVLRPGETLADLDAAALRVRLPWFVRGLHHAADRTPIGSRFV
                     RRFAHLCDRLSLPWREEVALVEDHPALMLGFALSTAAVLAVPVLNLAFRPIVIVGASH
                     VLGQLEATAVGGERVDEALQHGKPLLRGQ"
     gene            complement(2751..4004)
                     /locus_tag="VF516_20830"
     CDS             complement(2751..4004)
                     /locus_tag="VF516_20830"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEX8110194.1"
                     /translation="MSRKREIVPAMSLTTRVLLGLLAGLALGIALASWLPASWQPVAG
                     WIEPIGTIWTRALQLMVIPLVTTLLFTGVSAGGAGLRQLGAKGIGFFAITLAVVAAFT
                     LVVAPAVFARIPLDPGAADALRAASAAPHDAVPSFRDWLVGLMPSNLVAALSAGAMLP
                     IIVVTLIFAVAATRLPADMRERLERAAEAIKAVALIAVRGILSLAPYGVFALAVPLAL
                     RLGTTALGAVAMYVVMTAVLSMIAIAAMYPLAWLRGVPVRRFARAVAPAQGIAVSSRS
                     SLASLPALIDGASALGMPERVTGFFLPFSTSLFRVGAAVALPVGALFIAALYRVDLTA
                     AQRVTVGVTSVLLTFSAPGIPGGSILIMAPVLLSVGLPVEGVGILLGVDTIPDIFRTT
                     TNVTGSMTAAVVLDPPGRGERQADG"
     gene            complement(4091..5446)
                     /locus_tag="VF516_20835"
     CDS             complement(4091..5446)
                     /locus_tag="VF516_20835"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110195.1"
                     /translation="MTSGVARPSWRRPRAGHRASIRRENQDRLPVMRRNLGARALLGC
                     AILAACSSGHRIPGPDPDAGNDDGGPSAATRWAAVSAGGLHSIGIQSDGSLWGWGNAV
                     FGQVGKPCVSVAGCDQIEHLPVHLGTRTDWRVVSAGEEHSVALASDGTVWTWGGSGVG
                     EIGRVCADFACQSVPGMVGQDRDWVAISAGGRATFALKPDGSLWTWGSKNMCPSNCAD
                     PDAYNPVPVRVDAANDWALVAAGGQHVLAVKKDGSLWAWGRNYAGQLGDGKRDPDFLT
                     PGTAVPERVGTDTDWVAVAAGATHSLGLKADGTLWYWGTYDFLNSASLQFALAPTRLG
                     AERDWKIIAAGSRFGLAIKTDGSLWAWGNPFAGALGNGCGTCDGESQMVPARIGMDSD
                     WVAISGDRTDSFGDLDAHALGLRAVTASARTAWGWGLNRTCQLGVGAQPFPFASLPRP
                     L"
     gene            complement(5510..6547)
                     /locus_tag="VF516_20840"
     CDS             complement(5510..6547)
                     /locus_tag="VF516_20840"
                     /inference="COORDINATES: protein motif:HMM:NF022635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mbeg1-like protein"
                     /protein_id="HEX8110196.1"
                     /translation="MQAHDQQRSPRSDDAVELPPAQAPTPAVEVQPGAQKVPPAASFA
                     ELTGQPAVARAEPTTADPTRAEPAKAEAKAEDQPLTNADAVVEQLAHGMIDKELDARD
                     EHFLQVNGYQALPIIRGVHEFVMRTFLPTQSGKPPIVAFRGTRPSKIQTVIADLDPTG
                     IGMYQFNPNRGLIDAQMAAAASHGQVISSGHSLGGALAQIAAVTFPDRVQRIVTFQSP
                     GISREMVTELAKYNAEHPDHEIESSHHRVKGDLVPLGGQALTPGVIHNHEMTDGNLLQ
                     RNPLAKHTSMPLADEELAAGHDVPNHDDRHMRETGDVSTEQDNAHKGQFVEHLRTGLG
                     KLVYGLGHLKP"
     gene            complement(6552..>7384)
                     /locus_tag="VF516_20845"
     CDS             complement(6552..>7384)
                     /locus_tag="VF516_20845"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110197.1"
                     /translation="LDLAVRNQLHDRLIRLGRRIDATHGFADLVVDDETAASLREIVA
                     ALRERRKVRERWGFRGAAGVSVLFSGDPGVGKTMSATVIARQLNLAIYEIDLSRVVSK
                     WLGETEKNLGEVFDAAEPGHVVLLFNEADSLFGKRTTEVKSSNDRYANMETNYLLQRL
                     ERFGGLAILTTNLGGAVDPAFRRRFAYDVQFTFPDPEMRTELWRRAIPSTAEVGDIDF
                     EELGERFELSGGFIKVAAERSAFQAAATSEPISTQGLVVTIHRMYRERGKLTAVGRLE
                     "
BASE COUNT         1105 a         2675 c         2595 g         1009 t
ORIGIN      
        1 cgccgccgcg cccgccgcga ccagcagctc ggccggcagc gggttgggca gtgacaggat
       61 ccgcagatcg cgccgccggg cgcggatcag cgcgagcacc agcgcgatcg gcgcgcgccc
      121 gagcatgaag ccgccgggcg agatccaggc gccgtcggcg acggtcgccg ccgcctcgtc
      181 gaggccgcac cacgcggtcg cgccaccccc ggcccgttcg ccgggtaagc catgaagatc
      241 ctgggtcttt tcgttcatat ctacctacca gtcggtagct taccaaccgg tcagtatcaa
      301 ttctgacgct ccgggtcaag tgaattgcgg agcgagcgca cgccgaccgg gcatcccgcg
      361 ggcggaggta cactcgctcc ccatggctgg acagaggggg gggcagggca acgagcttgc
      421 gcgttgcgag cagacgcggc agcgcgtgct ccgcggcgat gcggccaccc tcgcggtgat
      481 ggtgatgacc gggcgaggtg gcgaccccga tcccgagcgc gccgagcagc tgttcgagcg
      541 cgccgaggag caaggcgtcg acgtcgaggc gtttcgcaac cagctcgggc tgtgatccgc
      601 ccgatgctcg cccgcctgtt ccgtgacctg gcgccgttcc agcccacgct gatcgggacc
      661 tacccgctcg gcctggccgt cgacggcagc gacctcgaca tcgcgtgcgc gtgcgacgat
      721 ctcgaccgct tcgagcacgc gctgcgcgcg acgctggccg cgctcggcgt cgccgcgcgc
      781 ttcgagcgca gcgagacggc ggtggtcgcg gcgttcgagc tcggcgacgt ccccgtcgag
      841 atcttcggcc aggcgctgcc ggtcaccgcg cagcgcggct tccgccacat ggtgatcgag
      901 ggccagctcc tggtgatcgg cggcgccgcg ctcgccgcgc gcatccgcga cctcaagcgc
      961 gccggtacca agaccgagcc ggccttcgcc caggtgctcg gcctcgccgg cgacccctac
     1021 gaggcgctgc tcgcgctgga ggcctggccg ccggatcgcc tgcgcgacct ggtcgcccgc
     1081 tcgctgcgcc cggccgttcc tccgtggatc ggggcctact ccggtgaccg ggcggcgctg
     1141 gtcccgctgt ttcgcatcgc cgacgattcg gaccaggcga tcgcgagcta cctcgcccgc
     1201 ggcacggtgc tggtcgccac cgacggcggc gcgccggtcg gccacgtcca gatgatcgag
     1261 cccgggcgcg gtcccggaag cacgcccggg aacgcgatcg acgaacccgc gacctgggag
     1321 ctcacgagcc tcgccgtcgt cgacagccat cgcgggcacg gcctcgggcg gcggctcgtc
     1381 agggccggca tcgcctacgc ccgcagccac ggcgcgtcgc gcgtcgtgct cgccacggcg
     1441 gccgccgata ccaccgtgct gcagttctat cagcgcattg gctttcgcct gctgcgcatc
     1501 gagcgcgacg cgttcacgcc cgcagcaggc tatccacccg agctgtgcgt cgacggtatc
     1561 cccctgctcg atcgcgtctg gctggacttc acgtggtagc tgcatcgatt ccgtccggcg
     1621 tggcccgggc gggactgtgg tatcttgcag acatgccgac caccggcgat cctgcgtctc
     1681 cacgcgatac cgatgacggt atcgacgtca cgctgatccg ctggatgctg tcactgtccc
     1741 ctgaggagcg gcttgccgtg ctgcagggct tcgtcgactc tctcgccgcc cacagccgtc
     1801 gcctccagct gcccgagcac gtggctggcg ccgacgatga cgatcggtcg gaacgccagg
     1861 ttcaggaccg gcaccgccag caccgccgcg gtcgacagcg cgaacccgag catgagcgcc
     1921 gggtggtcct cgaccagcgc gacctcctcg cgccagggca gcgatagccg gtcgcacaga
     1981 tgcgcgaacc ggcgcacgaa tcgactgccg atcggcgtgc ggtcggcggc gtggtgcagc
     2041 ccgcgcacga accacggcag ccgcacgcgc agcgccgcgg cgtcgaggtc ggcgagcgtc
     2101 tcccccggcc gcagcacgcg cgcggagtcg aacgcctcga cgaccaccca gtgcagcgcc
     2161 cacaccgcgg ccaccacctg gaccagcagc cagccggtcc ccggcacgag gtggagcagc
     2221 ccggtgaccg gcgcgagcgc gatcgcgatc aggatcgcct gcttggccgc gcgcccgagg
     2281 ttgcggcgga gcggctcgat gcacggctcg accggcccga agcccagcac cgagcgcgcc
     2341 tgcgccgcca gccgcgcgta gtggtcggcg agcacgatcg acggcagcgg cgcgagcacg
     2401 gcgaacgtgg tgtagaaccg gtgcaggaag ctgccgcggt ccccgagcgc cgcgacgacg
     2461 gcgcagcacg ccgccaagag gcccgccggc accagcgccg cccgcagcag atcgcgatgc
     2521 gccgccagcg tccgggctcc gaccaggggc tggaccagcc cgtagatcac ccggtcgacg
     2581 gcggcatccg gcggcggccg gggcgcaaca tggtcacgga cagcagcaaa gcggctgttg
     2641 gtgggcatcc gtctggccag tgtgggtacg ccgccgggtc aagtcgagcc cccaggccgc
     2701 ggacaccccg cgacgggggt gcgaccgcat ccgcggcgtc acggggccac tcacccgtca
     2761 gcttgccgct cgccccgccc cggcggatcg agcaccaccg cggcggtcat cgagccggtg
     2821 acgttggtgg tggtgcggaa gatgtcgggg atggtgtcga cgccgagcag gatgccgacg
     2881 ccttccaccg gcagcccgac cgacagcagc accggcgcca tgatcaggat cgagccgccg
     2941 gggatcccgg gcgccgagaa cgtcagcagc accgaggtca ccccgaccgt cacccgctgc
     3001 gccgcggtga gatcgacccg gtagagcgcg gcaatgaaca gcgcgcccac cggcagcgcc
     3061 accgccgccc ccacccggaa cagcgacgtc gagaacggca ggaagaagcc cgtgacccgc
     3121 tccggcatcc cgagcgccga cgcgccatcg atcagcgcgg gcagcgacgc cagcgacgac
     3181 cgcgacgaca ccgcgatccc ctgggccggc gcgacggcgc gggcgaaccg ccgcaccggc
     3241 acgccgcgca gccacgccag cggatacatc gcggcgatcg cgatcatcga tagcaccgcg
     3301 gtcatcacca cgtacatcgc gacggcgccg agcgcggtcg tgccgagccg gagcgcgagc
     3361 ggcaccgcga gggcgaacac cccgtacgga gcgagcgaca ggatcccgcg caccgcgatc
     3421 agcgcaaccg ccttgatcgc ctccgccgcg cgctccaggc gctcgcgcat gtccgccggc
     3481 agccgcgtcg ccgccaccgc gaagatcagc gtcaccacga tgatcggcag catggcgccc
     3541 gcggacagcg cggccaccag gttgctgggc atgagtccca ccagccagtc ccggaacgac
     3601 ggcacggcgt cgtgcggcgc cgcgctcgcc gctcggagcg cgtccgccgc gccggggtcc
     3661 agcgggatcc gcgcgaacac cgccggcgcc acgaccagcg tgaacgccgc caccaccgcc
     3721 agcgtgatcg cgaagaagcc gatccccttg gcgcccagct ggcggagccc ggccccgccc
     3781 gcgctcaccc cggtgaacag cagcgtcgtc accagcggga tcaccatcag ctgcagcgcc
     3841 cgcgtccaga tcgtgccgat cggttcgatc catccggcca ccggctgcca ggacgcggga
     3901 agccacgacg ccagcgcgat gcccagtgcg aggcccgcga gcaaacccag caacacccgg
     3961 gtggtcagcg acatggccgg cactatttca cgcttgcgtg acaacgaggt accggaggac
     4021 cgggcacctg acggcctcga ccggatagcg gcggaactgc gatcctcggg cgatggccat
     4081 cgagctcacg tcacagcggt cttggaaggc tcgcgaaggg aaagggttga gccccaacgc
     4141 cgagctggca cgtacggttc aggccccacc cccacgcggt tcgagccgag gcggtgaccg
     4201 cgcggagccc cagggcgtgt gcgtcgagat ctccgaaaga atcggtgcgg tcgccggaga
     4261 tggcgaccca gtcgctgtcc attccgatac gtgccggcac catctgggac tcgccatcgc
     4321 acgtaccaca gccattgccc agggctccgg cgaacggatt gccccatgcc cagagcgagc
     4381 catccgtctt gatcgcgagc ccgaaccgcg aaccggcggc gatgatcttc cagtcgcgct
     4441 ccgcgccgag gcgcgtgggt gcgagcgcga actgcaacga agcgctgttg aggaaatcgt
     4501 acgtacccca gtaccagagc gttccatcgg ccttgagccc caaggagtgc gtggccccgg
     4561 cggccacggc gacccagtcg gtgtccgtcc cgacgcgctc ggggaccgcc gtacccggcg
     4621 tgaggaaatc gggatcgcgc ttcccgtcac cgagctgtcc ggcgtagttg cgcccccagg
     4681 cccagagcga tccatctttc ttgaccgcga gcacatgctg gccgccggcg gcaacgagcg
     4741 cccagtcatt cgcggcgtcg acgcgcaccg gcaccgggtt gtacgcgtcc ggatcggcgc
     4801 agttggaagg gcacatgttc ttgctccccc acgtccacag gctcccgtcg ggcttcaacg
     4861 cgaaggtcgc ccttcctccc gccgagatcg cgacccaatc gcggtcctgc ccgaccatcc
     4921 cggggaccga ctggcaggcg aagtccgcgc acacgcggcc gatctccccg acgccagagc
     4981 ctccccatgt ccagaccgtt ccatcgctcg cgagggcgac gctgtgctcc tcgccggcgc
     5041 tcacgacgcg ccagtcggtg cgggtcccga ggtgtacagg caggtgctcg atctggtcgc
     5101 acccggccac cgaaacgcac ggtttgccga cctgcccgaa gacggcgttt ccccatcccc
     5161 acagggagcc atcgctctgg attccaatcg agtgcaagcc cccagccgag acggccgccc
     5221 agcgtgtcgc cgcggacgga cctccgtcgt cgttcccggc gtcgggatcg ggacccggta
     5281 tccggtgccc cgaggagcac gccgcgagga tcgcgcaacc aagcagcgct cgcgcaccga
     5341 ggttccgtcg catgaccggg agcctatcct ggttttcgcg ccggatggac gcgcgatgtc
     5401 ctgcacgagg acgccgccac gacgggcgag cgaccccgga ggtcatcgct gtgcgtccga
     5461 tcggccgagt cggccgcaca cgcgcggcgc gccaccggcc ccggtcgcgc tacggcttca
     5521 ggtggccgag gccgtagacc agcttgccca ggccggtgcg caggtgctcg acgaactggc
     5581 ccttgtgggc gttgtcctgc tcggtggaga cgtcgccggt ctcgcgcatg tggcggtcgt
     5641 cgtggttcgg cacgtcgtgg ccggcggcga gctcctcgtc ggcgagcggc atcgacgtgt
     5701 gcttggcgag cgggttgcgc tgcaagaggt tgccgtcggt catctcgtgg ttgtggatga
     5761 cgccgggggt cagcgcctgg ccgccgagcg ggaccaggtc gcccttgacg cggtggtgcg
     5821 aggactcgat ctcgtgatcg gggtgctcgg cgttgtactt ggcgagctcg gtgaccatct
     5881 cgcgggagat gccgggggac tggaaggtca cgatgcgctg gacgcgatcg gggaaggtga
     5941 cggcggcgat ctgggcgagc gcgccgccga ggctgtggcc ggaggagatg acctggccgt
     6001 gcgacgcggc ggcggccatc tgggcgtcga tcaggccgcg gttggggttg aactggtaca
     6061 tgccgatgcc ggtcggatcg aggtcggcga tcaccgtctg gatcttgctc ggccgggtgc
     6121 cccggaacgc gacgatcggc ggcttgccgg actgggtcgg caggaacgtg cgcatcacga
     6181 actcgtggac gccgcggatg atcggcaggg cctgatagcc gttgacctgc aggaagtgct
     6241 cgtcccgggc gtcgagctcc ttgtcgatca tgccgtgggc gagctgctcg acgacggcgt
     6301 cggcgttggt cagcggctgg tcctcggcct tggcttccgc cttggcgggc tcggccctgg
     6361 tcgggtcggc cgtggtgggc tcggccctgg cgaccgcggg ctggccggtg agctcggcga
     6421 agctggcggc cggcgggacc ttctgcgcgc cgggctggac ctcgaccgcg ggcgtcgggg
     6481 cctgggccgg ggggagctcg acggcgtcgt cggagcgcgg gctgcgttgc tgatcgtgtg
     6541 cttgcatggg gtcactccag cctgccaacg gcggtcaatt tgcctcgctc gcgatacatc
     6601 cggtggatgg tcacgaccag gccctgggtg gatatcggct cgctcgtcgc ggcggcctgg
     6661 aacgccgagc gctcggcggc aactttgatg aaccccccgg acagctcgaa gcgctcgccg
     6721 agctcctcga agtcgatgtc gccgacctcg gcggtggagg gaatcgcccg ccgccacagc
     6781 tccgtgcgca tctcggggtc gggaaacgtg aactggacgt cgtacgcgaa ccgccggcgg
     6841 aacgccggat ccaccgcgcc acccaggttg gtggtcagga tcgccagacc gccgaaccgc
     6901 tccaggcgct gcaagaggta gttggtctcc atgttcgcgt agcgatcgtt ggatgacttg
     6961 acctccgtcg tacgcttgcc gaacagagaa tctgcctcgt tgaacagcaa aaccacgtgg
     7021 ccgggctcgg ccgcgtcgaa tacctcgccc aggttcttct cggtctcgcc cagccacttg
     7081 gagaccaccc gggacagatc gatctcgtag atcgccaggt tgagctggcg ggcgatcacc
     7141 gtcgcgctca tcgtcttgcc gactcccgga tcgccggaga acagcaccga gacgccggcg
     7201 gcgccgcgaa atccccagcg ctcccggacc tttcggcgct cacgcagcgc ggcaacgatc
     7261 tcgcggagcg aggccgcggt ctcgtcgtcg accacgaggt cggcgaaccc gtgggtcgcg
     7321 tcgatccggc ggccgaggcg gatcaggcgg tcgtggagct ggttgcgcac cgcgagatcg
     7381 agcg
//
LOCUS       DASVOR010001086         5450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_127693,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001086 DASVOR010000000
VERSION     DASVOR010001086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127693"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            566..1039
                     /locus_tag="VF516_20850"
     CDS             566..1039
                     /locus_tag="VF516_20850"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8110198.1"
                     /translation="MMITGRLWKERTGWLAESEIADVCTQGRSRKDAARMLADAFETL
                     IDRPGCRVTVTDGGRADGEVTIEASEPAALAAFVLQRLRQNSGKSLAEVAHAMGRSSK
                     NAFARYEQGRAVPTIDMFDELLRAVSSDTTLIIGSRRQGVRKPPLPARRRKRRAA"
     gene            1190..1390
                     /locus_tag="VF516_20855"
     CDS             1190..1390
                     /locus_tag="VF516_20855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110199.1"
                     /translation="MRGDKLVALADEDVNVRREPESIRHEFAKDDKCVKRCPQLGTYK
                     VLGYELLRVVGSARTITALPAF"
     gene            complement(1415..1738)
                     /locus_tag="VF516_20860"
     CDS             complement(1415..1738)
                     /locus_tag="VF516_20860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110200.1"
                     /translation="MPYATLHYRLRAAERALPEDVEGFLRTWGTEIWAAGARQITLFL
                     KDLPAELRDTMIARRAEDWILVAAPDGALMTCYRRRGAWRFVTRKSEDHRPRRPRHRR
                     PHRHH"
     gene            2183..2443
                     /locus_tag="VF516_20865"
     CDS             2183..2443
                     /locus_tag="VF516_20865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110201.1"
                     /translation="MSNFTTIALTDLDLVTGGEGEDTGIFGPGAGPNRENIEGSAGFQ
                     TPVGVKVEGSGKYQTTASDQQACIQTAIKNGADPLAAVKACR"
     gene            2440..2703
                     /locus_tag="VF516_20870"
     CDS             2440..2703
                     /locus_tag="VF516_20870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110202.1"
                     /translation="MNAVPVVLDAAALAGVTGGADNETHIQTPDGTRIDKSQTDRRYA
                     QEYATQACTQANTRLFGLWHDDPTPCISDFMKKYDQQSAGGTP"
     gene            2700..2963
                     /locus_tag="VF516_20875"
     CDS             2700..2963
                     /locus_tag="VF516_20875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110203.1"
                     /translation="MIALDRDLLAGVVGGGDDAGGEELPGPNRTSTEGRIRVETPLYS
                     TGWDTGKTTTAKTNYASCLATLPDGTPTAEIRDACGTPNHPKY"
     gene            2972..3232
                     /locus_tag="VF516_20880"
     CDS             2972..3232
                     /locus_tag="VF516_20880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110204.1"
                     /translation="MLDHIDDDALAAVCGGTGVNTENTNTTGSDVSIKRSNMAVCLDD
                     RASRCQNDPKNTWMFGLLPDQQKIGQCKAVTAPEQCFDPPAQ"
     gene            complement(3247..4116)
                     /locus_tag="VF516_20885"
     CDS             complement(3247..4116)
                     /locus_tag="VF516_20885"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8110205.1"
                     /translation="MTCGTIAIGDFQVCRLGFGTMRLVGHGVMGPPPDIAAARETLRL
                     LPELGVNLVDTANAYGPLLAEWLVRAVLHPYRGLLVATKGGVLRPGPFEWQADGRPEA
                     LRAAVQGSLATLGVDRIDLWQLHRIDPAVPADEQFAAIAEMQREGLIRHVGLSNVWPE
                     QIEAARKHFTVAAVQNLYHVIDRRSEPVVEYCEQHGIPFIAYFPLATGALAARDSILQ
                     HAAAKIGITPGQAALAWLLKRSPSIVVIPGTQNPDHLRENVAAASIELSDEHYAEIER
                     IGRKADQLRAQRR"
     gene            complement(4113..>5450)
                     /locus_tag="VF516_20890"
     CDS             complement(4113..>5450)
                     /locus_tag="VF516_20890"
                     /inference="COORDINATES: protein motif:HMM:NF036482.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HEX8110206.1"
                     /translation="LYDELDAQGVLLWQDLMFANMDYPDDPAFVAGVEAEVDQQLARL
                     AGRPSLAIVCGNSEGEQQAAMWGAARELWTPRLFHEVLARRCRDVPYVASSATGGAFP
                     HAVNAGPSSYYGVGAYLRPVDDARRAEVRFASECLAFANIPDDPSLPPVHDPAWKAGT
                     PRDRGADWDFDDVRDHYVQRVFGIEPAALRVADPDRYLELARVATGEVMARVFDDWRR
                     ARSTCRGGLVWFLRDLQPGAGFGLIDVRGIPKPCWYVVRRALAPIGLALTDEGTNGLA
                     IHVVNDRAEPLAGRLELALWRGDVRLRDAATEVAVAPHAAIELAAGALFDGWIDLSYA
                     YRFGPPAADVVHARLRAGDDVAEAHWFPAGLSAAREPDVGLAVTGDAARIAITTRRFA
                     RFVAIDAPGYVPSDNYFHLAPDQRRTITVTGEGPLRVAIRALNSEVGATGGSP"
BASE COUNT          889 a         1904 c         1895 g          762 t
ORIGIN      
        1 ctcactgacg ttgatctcgt tggcgagcgc gatctgcagc agctgcgccg cgtcgcccgg
       61 cgcatcggcg gtgagcgagg tgcgcccggc gccgaggatc ggtgcgagcc gttcggactt
      121 gaaggcttcc agctggtcga cgcacgccat gcccttgcat agcacccgcg cgcggtcgat
      181 ggggccgcgg agcggtccgt ccggagatcc ggcaccgggt tgatcggcca ggccaacgcg
      241 gcgctgccgg gcgtggctgc ccctgagcgt cgggcgccga tgtggattcg aggctgcgat
      301 cttgcgcgat gagcaggggg ggcgagcttg gcatcgtacc ccgatgtgtg tacgatactc
      361 cggtgaagcg acaggagctg gagtcggcgc tacgtgcgct ggggtggcat ttcctcaagc
      421 atggcggcag ccatgacttg tgggttcatc ccgagcgcag ccgcaagctt gcagtgccgc
      481 gtcacaagga atcaaggagt acctcgcccg gagcctgctt cggcaggccg agatggcaga
      541 ctgatcggag catcggagca ccaccatgat gatcacgggt cgactctgga aagagaggac
      601 gggatggctc gccgagtccg agatcgcgga cgtctgcacg caggggcgat cccggaagga
      661 cgcggcgcgg atgctcgccg acgcgttcga gacgctgatc gatcggcccg gttgcagggt
      721 gaccgtcacc gatggcgggc gggccgacgg cgaggtgacg atcgaagcca gcgaaccggc
      781 agcgctcgct gccttcgtgc tgcagcggtt gcgccagaac agcggcaagt cactggcgga
      841 ggtggcgcac gcgatgggtc gatcgtcgaa gaatgcgttc gcgcgctacg agcagggacg
      901 ggctgtcccg acgatcgaca tgttcgacga gttgctccgc gccgtctcca gcgacaccac
      961 gctgattatc ggttcgcggc ggcagggtgt caggaaacca ccgctgcccg cgaggcgccg
     1021 gaaacgccgg gcagcatgag cctccggact tcgtcctgtg ccagaaggcc atcggggctc
     1081 gggctccgac ggccgagcgt cgggtgcacc cccatggctc gttctcgacg agacatcgat
     1141 cgagcatggg ctgaaccaca accatggcaa ggcgtacgtc tggttcggct tgcgcggtga
     1201 caagctcgtc gcgctcgcgg acgaggacgt gaacgtccga cgcgagcccg agtcgatcag
     1261 gcacgaattc gccaaggacg acaagtgtgt gaagcgttgc ccgcaactcg gaacgtacaa
     1321 ggtcctcggt tacgagttgc tccgcgtcgt cggttcggcg cgcacgatca cggcgctgcc
     1381 ggcgttctga ccatagtccg cgatcgacac ctcttcagtg gtggcggtgc ggccgccggt
     1441 gtcgcggcct gcgtggtcga tgatcctcgg acttgcgcgt gacgaagcgc cacgcgccgc
     1501 ggcgccggta gcaggtcatc agcgcgccgt cgggggccgc gacgaggatc cagtcttcgg
     1561 cgcgccgggc gatcatggtg tcgcgcagct ccgccggcag gtccttgagg aacagggtga
     1621 tctgtcgcgc acccgccgcc cagatctcgg tgccccacgt ccgcagaaac ccctcgacgt
     1681 cctccggcag cgcacgttcg gcggcgcgga ggcgataatg cagcgtggca tagggcatcg
     1741 gggcgagcat gtggatcccc catctcaagg aggatgccag gcgcaaacct ggcgtcattg
     1801 ctcgcgatcg gttgccggag ccgagcaaaa cgcgcaccgc ttgcgtggcc tgcctggttt
     1861 ttgcgcgagg ctcagcgccg ttcagccccg atcgcggcag gacgcgctgg ccaacgcgcg
     1921 ctggcgggct cgcggccggc tctccggtcc ctcgctccat gccgggccgg gtcgtgctgg
     1981 ctacccatcc cgtccagatc gagcggccgc gctgggatgc attccagcgg cgacagcttg
     2041 ccgaccctgg gtagcaccca cggcccccgg caggcgcgcc aagtcatcga gatcgcagca
     2101 tttgccgtgt ggcaccgggt ctgcacaagg gttcctcgca acccgaaacc caccactctc
     2161 acgaatcacc tggaggctca ccatgtcgaa cttcaccacc atcgcgctca cggatctcga
     2221 tctcgtcacc ggcggcgagg gcgaggacac gggcatcttc ggaccgggcg ccggtcccaa
     2281 ccgcgagaac atcgagggca gcgccgggtt ccagaccccg gtcggcgtga aggtcgaagg
     2341 cagcggcaag taccagacca ccgcgtccga ccagcaggcg tgcatccaga ccgcgatcaa
     2401 gaacggcgcg gatccgctcg cggcggtcaa ggcgtgccgg tgaacgccgt gcccgtcgtc
     2461 ctcgatgccg cggcgctcgc cggggtgacc ggaggcgccg acaacgagac ccacatccag
     2521 actccggacg gcacccggat cgacaagagc cagaccgatc gccggtacgc gcaggaatac
     2581 gcgacccagg cgtgcaccca ggcaaacacc cgcttgttcg gcctgtggca tgacgatccg
     2641 acgccgtgca tcagcgactt catgaagaag tacgaccagc agagcgctgg aggcacgcca
     2701 tgatcgccct cgaccgtgat ctgctcgccg gcgtggtcgg cggcggagac gacgccggtg
     2761 gcgaggagct gcccggcccg aaccggacct cgaccgaggg tcgcatccgg gtcgagacgc
     2821 cgctctacag caccggctgg gacaccggca agaccacgac ggcaaagacc aactacgcgt
     2881 cctgcctcgc gactctgccg gatggcacgc caactgccga gatccgcgat gcctgcggca
     2941 cgccgaacca ccccaagtac tgaggtccac catgctcgat cacatcgatg acgacgcgct
     3001 cgccgccgtg tgcggtggca ccggcgtcaa caccgagaac accaacacga ccggcagcga
     3061 cgtgagtatc aagcgctcca acatggcggt gtgcctcgac gaccgtgcga gccgctgcca
     3121 gaatgacccg aagaacacct ggatgttcgg cctgctcccc gatcagcaga agatcgggca
     3181 atgcaaggcg gtgaccgcgc cggagcaatg tttcgacccc ccggcgcagt gaccgtccgc
     3241 gcgagcctac ctgcgctggg cgcgtagctg atcggccttc cggccgatcc gttcgatctc
     3301 cgcgtagtgc tcgtcgctga gctcgatcga tgcggcggcg acgttctcgc gcaggtggtc
     3361 cgggttctgc gtccccggga tcacgacgat cgacggcgac cgcttcaata accacgcgag
     3421 cgcggcctgg cccggtgtga taccgatctt cgccgcggcg tgctggagga tcgaatcgcg
     3481 cgcggcgagc gcaccggtcg cgagcgggaa atacgcgatg aacgggatgc cgtgctgctc
     3541 gcagtactcg acgaccggtt cgctccgtcg atcgatcacg tggtagaggt tctgcacggc
     3601 ggcgacggtg aagtgcttgc gcgccgcctc gatctgctcg ggccacacgt tggacaggcc
     3661 gacgtgccgg atcagtccct cccgctgcat ctcggcgatc gcggcgaact gctcgtcggc
     3721 cggcaccgcc ggatcgatgc ggtggagctg ccacagatcg atgcggtcga caccgagcgt
     3781 cgcgaggctg ccctggaccg ccgcgcgcag cgcctccggg cgaccgtcgg cctgccattc
     3841 aaacggcccg ggccgcagga ccccgccctt ggtcgcgacg agcagcccgc gatacgggtg
     3901 cagcaccgcg cgcacgagcc attcggcgag cagcgggccg tacgcgttgg ccgtgtcgac
     3961 caggttcacg ccgagctccg gcagcagccg gagcgtctcg cgcgccgcgg cgatatcggg
     4021 cggcggcccc atcaccccgt ggccgaccag gcgcatcgtg ccgaagccga gccggcagac
     4081 ctggaagtcc ccgatcgcga tcgtcccgca cgtcatggcg agcctcccgt ggcaccgacc
     4141 tcgctgttga gcgcgcggat cgcgacccgg agcgggccct cgcccgtgac ggtgatcgtc
     4201 cggcgctgat ccggcgcgag gtggaagtag ttgtccgacg gcacgtaccc cggcgcatcg
     4261 atcgcgacga agcgagcgaa ccggcgcgtc gtgattgcga tcctggcagc gtcgccggtc
     4321 accgcgagcc cgacgtccgg ctcgcgcgcg gcggacaatc ccgccgggaa ccagtgggcc
     4381 tcggcgacat cgtcgccggc gcgcagccgc gcgtggacga cgtccgccgc gggcggcccg
     4441 aaccggtacg cgtacgacag atcgatccag ccgtcgaaca gcgcgcccgc ggcgagctcg
     4501 atcgcggcgt gcggggcgac cgcgacctcg gtcgccgcgt cgcgcaggcg aacgtcgccg
     4561 cgccacagcg ccagctcgag ccggcccgcg agcggctccg cgcgatcgtt cacgacgtgg
     4621 atcgcgaggc cgttggtgcc ctcatcggtg agcgcgagcc cgatcggcgc cagcgcgcgg
     4681 cgcacgacat accagcacgg cttcgggatc ccgcgcacgt cgatcagccc gaagccggca
     4741 cccggctgca ggtcgcgcaa gaaccacacg aggccgccgc ggcacgtcga tcgcgcgcgc
     4801 cgccagtcgt cgaacacgcg cgccatcacc tcgccggtcg cgacgcgcgc cagctcgaga
     4861 tagcggtcgg gatcggcgac gcgcagcgcg gccggctcga tcccgaacac gcgctgcacg
     4921 tagtggtcgc gaacgtcgtc gaagtcccag tcggcgcctc gatcgcgcgg cgtgccggcc
     4981 ttccacgccg gatcgtgcac gggcggaagg ctcggatcgt cggggatgtt cgcgaacgcg
     5041 aggcactccg acgcgaagcg cacctcggcg cgccgcgcgt cgtcgaccgg gcggaggtac
     5101 gcgccgacgc cgtagtacga cgacggcccc gcgttcacgg catgcggaaa cgcgccgccg
     5161 gtcgcgctcg acgcgacgta cgggacgtcc cggcaccgcc gcgcgagcac ctcgtggaac
     5221 agccgcggtg tccacagctc gcgcgcggcg ccccacatcg ccgcctgctg ctcgccctcg
     5281 ctgttgccgc acacgatcgc cagcgacggc cgccccgcga gccgcgcgag ctgctggtcg
     5341 acctcggcct cgacgccggc gacgaaggcg gggtcgtcgg ggtagtccat gttcgcgaac
     5401 atcaggtcct gccagagcag cacgccctgc gcgtcgagct cgtcgtagag
//
LOCUS       DASVOR010001087         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_361780,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001087 DASVOR010000000
VERSION     DASVOR010001087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361780"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..794)
                     /locus_tag="VF516_20895"
     CDS             complement(<1..794)
                     /locus_tag="VF516_20895"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8110207.1"
                     /translation="MGTPFSVLARCEHKNVEPASAAPTSRLWASIKIDPLGGGLERER
                     APLAVALVADVSGSMQGPPIEHVIRSCEILIDLLGERDQLAIVTFASDAQVRCGLTWV
                     DAAGRTQLRRALGALSADSNTNLHGGLGVAAGVLLTAPAGLRRAMVVMSDGQPNVGLS
                     SPSDLAGYVTGLKLAVSSLGFGRSHDENVLDAIATAGSGRYAYVPDPVLARVDLARAA
                     LAHGGIVADQLELRLQPTDGVELVQVLPASPLRVGSHGVTAAVGDV"
     gene            1016..>1688
                     /locus_tag="VF516_20900"
     CDS             1016..>1688
                     /locus_tag="VF516_20900"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8110208.1"
                     /translation="MDTPDSAPERRRWAKVIDQTRCIGCHACSTACKSENGVPLGVHR
                     TYVKAVEIGAFPTVRRAFQVTRCNQCDDAPCTAVCPTRAMFRRADGIVDFDKRICIGC
                     KACIAACPYDAIFINPDDGAAEKCNFCSHRVDAGLEPACVTVCPTGAIVIGDLDEPGS
                     PVARIVQRDAVAVRRPEKETRPKLFYKGAHQATLDPLAARRPPGGLLMWSEQPDVPHG
                     VPAGHP"
BASE COUNT          239 a          607 c          628 g          214 t
ORIGIN      
        1 aacacgtcgc cgaccgcggc ggtgacgccg tggctgccga cccgcagcgg gctggccggc
       61 aggacctgga ccagctcgac gccgtcggtc ggctggagcc ggagctcgag ctggtcggcg
      121 acgatgccgc cgtgggcgag cgcggcgcgc gcgagatcga cccgggcgag caccggatcg
      181 gggacgtagg cgtagcggcc cgagccggcg gtggcgatcg cgtcgagcac gttctcgtcg
      241 tgggatctcc cgaacccgag cgacgacacc gcgagcttga gcccggtgac gtagccggcg
      301 agatcggacg gcgacgacag cccgacgttg ggctggccat cgctcatcac gaccatcgcg
      361 cggcgcagcc cggccggcgc ggtcaaaagc acgccggcgg cgaccccgag cccgccgtgc
      421 aggttggtgt tgctgtccgc cgacagcgcg ccgagcgcgc ggcgcagctg ggtcctgccg
      481 gcggcgtcga cccaagtcag gccgcagcgg acctgggcgt cgctggcgaa cgtcacgatc
      541 gcgagctggt cgcgctcgcc gagcaggtcg atcaggatct cgcagctgcg gatcacgtgc
      601 tcgatcggcg ggccctgcat cgagcccgac acgtcggcga ccagcgccac cgcgagcggc
      661 gcgcgctcgc gctccaggcc gccgccgagc ggatcgatct tgatcgacgc ccacagccgg
      721 gaggtaggag ctgcgctggc cggctcgacg ttcttgtgct cgcagcgcgc gagcacgctg
      781 aatggtgtcc ccatgcccag tgcgtatagg cgacggtgga cggcgcgcta gcaatttttc
      841 gcggcgcggg acgtggggcc gcgtccgaca cgccgggagc gcgggcggcg cggacgtttc
      901 aagacgtttc acgaccgcgc ggcgaaccgg gtgatttcgc cgatcagcgc gccggtgcgc
      961 ttcctgcaac gcgccaggcg ggggctgcgc ctggcctccg cctggccacc cgccgatgga
     1021 cactcccgac tccgcgcccg agcgccgccg ctgggcaaaa gtgatcgacc agacccgctg
     1081 catcggctgc cacgcgtgca gcaccgcgtg caagtcggag aacggcgtgc cgctcggcgt
     1141 tcaccggacc tacgtcaagg cggtcgagat cggcgcgttc ccgaccgtgc ggcgcgcgtt
     1201 ccaggtcacg cgctgcaacc agtgcgacga tgcgccgtgc accgcggtgt gcccgacccg
     1261 cgcgatgttc cgccgcgcgg acggcatcgt cgacttcgac aagcggatct gcatcggctg
     1321 caaggcgtgc atcgcggcgt gcccgtacga cgcgatcttc atcaaccccg acgacggcgc
     1381 ggcggagaag tgcaacttct gctcgcaccg ggtcgacgcc gggctcgagc cggcgtgcgt
     1441 cacggtgtgc ccgaccggcg cgatcgtgat cggcgatctc gacgagccgg gctcgccggt
     1501 ggcgcggatc gtccagcgcg acgcggtcgc ggtgcgccgg cccgagaagg agacccggcc
     1561 caagctgttc tacaagggcg cgcaccaggc gacgctcgat ccgctcgccg cgcgccggcc
     1621 gccgggcggg ctgctgatgt ggagcgagca gccggacgtg ccgcacggcg tacccgccgg
     1681 ccacccgg
//
LOCUS       DASVOR010001088         2770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_11723,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001088 DASVOR010000000
VERSION     DASVOR010001088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11723"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            361..1875
                     /locus_tag="VF516_20905"
     CDS             361..1875
                     /locus_tag="VF516_20905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110209.1"
                     /translation="MQKIDVLKRWRPIDKSLLPGFLFPPAHVRILMYAEGGIQFTGGS
                     FQGLTHVLATLAADPWFWVQFDVTKANRGADPSADRQNVKLTDFTLSASFDEIWLFGF
                     SSGDLLTPQEKAALADFMDNHKGGVLATGDHASLGQGIAGAVPRAGKMRLYPAPPSVP
                     PGWNTTIVEGSDPNSTYDFNDQSDDVPQRIRWKKYPLWSLITTPFNRRVRPHPLLCSP
                     EGPIDVFPDHEHEGEAIVPSSFPAAEWPSGPTGQVRPEVIAWGRIKDPAATKTGQEIG
                     LVSAYDGHEASVGRIAADSTWHHFFDINLDGLPGDPTYRGFSATASGQAALKKIEAYY
                     LNLAVWLAPPSKQAQMRSAASWRILWTDRFAELATSEVLLKDSAIAHLLVGEQAIDAL
                     GRVAPVCTVHQWLYVDLLREINPEILVRARELLEHDPVVPHLLGHFAVGAAVTELIRQ
                     FGPGAHGAEPPKGPPDAEAFHKTLVKAAARGFASTQDYFSGLANSVGKIVGRCP"
     gene            2052..2630
                     /locus_tag="VF516_20910"
     CDS             2052..2630
                     /locus_tag="VF516_20910"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HEX8110210.1"
                     /translation="MLTGVIVVVVLSLVVGVPRLLRQTARQKKLRKIAVAKIGTLAPG
                     PHIVRVVGTARAVGAATTAGPYSGTPGVAAVFERWDSQRIQRLDRQVRHVPFVVEDET
                     GSVQIALEHADFALELLPVKLAETGPNRVFGAGIIAANREAAGDTLECVVKPGDRLAV
                     VGYVYARPDGLVLAGTAASPVVVSNDPVLLAS"
BASE COUNT          423 a          961 c          956 g          430 t
ORIGIN      
        1 ctccggctgc gcggcgccca ccacgcgctg gccgacggcc tcgccgacgt cgcgtcgccc
       61 gtggcggtcc agctgctgca gcggatcgcc gccggcaagg aggcgccgga acgcgccagg
      121 gccgtgctcg ccgcccgcgg cgtttgaccc ggatcatcca ccacgttgcg ctgcgaacat
      181 cgcagcgtgg gcagctcgtc gcacggtcgc gttcgaacct ccggccgtcc ttgccatcgc
      241 gacgatcgcc gttgcacgtg ccgagaagaa acatcggccg ctacgtctat ccggccaggg
      301 gctggaggcg cggtgcctgc acggctcccg ggaggtcata tggggtccga tatcttcgag
      361 ttgcagaaaa tcgatgtgct gaagcgctgg aggccgatcg acaagtcgct gttgccaggc
      421 tttttgtttc cgccggctca cgttcgcatc ctgatgtacg ccgagggtgg catccagttc
      481 acgggcggat cgtttcaggg gctgacccac gtcctggcga cgctggcagc cgatccgtgg
      541 ttctgggtcc agttcgacgt gaccaaggcc aaccgcggcg cggatccgtc cgcagatcgc
      601 cagaacgtga agctcacgga tttcaccctc tcggcgagct tcgacgagat ctggctgttc
      661 gggttcagct cgggcgatct gctcacgccg caggagaagg ccgccctcgc ggacttcatg
      721 gacaaccaca agggcggcgt gctcgcgacc ggcgaccacg cgtcgctcgg ccaggggatc
      781 gccggtgccg tgccgcgcgc aggaaagatg cggctctatc ccgcgccccc gagcgtgcct
      841 cccggatgga acacgacgat cgtcgagggc tcggacccca actcgacgta cgacttcaac
      901 gatcaatccg acgacgtgcc gcaacggatc cgctggaaga aatacccgtt gtggtcgctc
      961 atcaccacgc cgttcaaccg ccgcgtccgc ccgcacccgc tgctgtgcag ccccgagggt
     1021 ccgatcgacg tgttccccga ccacgagcat gagggcgagg ccatcgtgcc atcgagcttc
     1081 ccggcggccg agtggccgtc cggccccacc ggccaggtcc ggcccgaggt catcgcatgg
     1141 ggccgcatca aggaccccgc cgccacgaag accggccagg agatcggcct cgtctctgcc
     1201 tacgacgggc acgaggcgtc ggtgggtcgc atcgcggccg actccacctg gcatcacttc
     1261 ttcgacatca acctcgacgg gctgcccgga gatccgacct accgcgggtt ctcggcgacc
     1321 gccagcggcc aggcggcgct gaagaagatc gaggcgtact acctcaacct cgcggtctgg
     1381 ctcgcgccgc cgagcaagca ggcgcagatg cggtccgccg cgtcctggcg catcctctgg
     1441 accgatcgct tcgccgagct ggcgaccagc gaggtgctgc tcaaggactc ggcgatcgcg
     1501 cacctgctcg tcggcgagca ggcgatcgac gcgctcgggc gagtcgcgcc ggtgtgcacc
     1561 gtgcaccagt ggctctacgt cgacctgttg cgcgagatca acccggagat cctcgtgcgc
     1621 gcgcgagagc tcctcgagca cgatccggtc gtgcctcact tgctgggcca cttcgccgtg
     1681 ggagcggcgg tgaccgagct catccgccag ttcggcccgg gcgctcacgg cgccgagccg
     1741 cccaagggac cgcccgacgc cgaggcgttt cacaagaccc tcgtgaaggc ggccgcccgc
     1801 ggcttcgcgt cgacccagga ctacttcagc gggctcgcga acagcgtcgg aaagatcgtc
     1861 ggcaggtgtc cgtagccgcg gatcgaagtc cagtcacgat ccggtagcag cccgcggcct
     1921 gtagcgagga catcgagtcg agggcaggtg gcaattccgg agttggacca gcgcagtgcc
     1981 gatggatgcc gggccgcggg atggcattgc ccgcgccccc tgtgaggcgc gagctgtgat
     2041 aaccacggtg tgtgttgacc ggagtcatcg tggtggtcgt gctgtctctc gtggtcgggg
     2101 taccgcggct gctccggcaa accgcgcggc agaagaagct ccgcaagatc gcggtcgcca
     2161 agatcggcac cctggcgccc ggcccgcaca tcgttcgcgt cgtcgggacg gcgcgagcgg
     2221 tcggtgccgc gacgaccgca gggccgtatt cgggtacgcc cggcgttgct gccgtgttcg
     2281 agcgctggga cagccagcgg atccagcggc tcgatcggca ggtgcggcat gtaccgtttg
     2341 tggtcgagga tgagaccggc tcggtccaga tcgcactcga gcatgcggac ttcgcgctcg
     2401 agttgcttcc ggtgaagctc gcggagaccg gtccgaaccg ggtgttcgga gcgggcatca
     2461 tcgccgcgaa ccgcgaggct gcgggagaca ccctggagtg cgtggtgaag cccggcgacc
     2521 ggctcgcggt ggtcggctac gtctacgccc gcccggacgg gctcgtgctg gccgggactg
     2581 cagcgtcgcc cgtggtcgtg agcaatgatc ccgtgctgct ggcgagctga gccggcagtt
     2641 gctatcgggc acccgctgga cccagcgccg cgagctcgcc gcagatcgcc gcgggcagca
     2701 ccaccgtgtc agcccccaca cgcgccgggc agcccgaggc gcgcggtgcg ccgagctcca
     2761 gcgcgtgctg
//
LOCUS       DASVOR010001089         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_332376,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001089 DASVOR010000000
VERSION     DASVOR010001089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332376"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1418)
                     /gene="mnmG"
                     /locus_tag="VF516_20915"
     CDS             complement(<1..1418)
                     /gene="mnmG"
                     /locus_tag="VF516_20915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEX8110211.1"
                     /translation="MSYEYPDSYDAIVVGAGHAGCEAALAAARMGARVLALTGSLDMV
                     AQMSCNPAIGGVAKGHLVKEIDALGGEMASVIDETGIQFRRLNASKGPAVRSTRAQAD
                     KRRYRDEMRMRLERTDRLALRQGEVAQLHTDDGSGRPRVVGVTTTMGVRYRARSVILT
                     TGTFLRGAIFVGEARAAGGRAGEAPAVSLSHSLDALGFPLARLKTGTPCRLDRRTIDV
                     AGLELQPGDDPPPMFRWGGRDRPPLPQVPCWITYTTERTHAIIRDGLPRSPLYRKEIS
                     GRGPRYCPSIEDKIVRFADKERHQIFLEPEGVEVGLGGAGEVYPNGISTSLPFDVQLA
                     FLRSIPGLERAEMTRPGYAVEYDFIDPREVLPTLETRRVHGLYFAGQINGTSGYEEAA
                     VQGLLAGINAALALGFGPGDREPLILRRDQAYGGVLVDDLTTRGTSEPYRMMTSRAEF
                     RLLLREDNTADRLMPIGRWLGLV"
     gene            complement(1465..>2123)
                     /locus_tag="VF516_20920"
     CDS             complement(1465..>2123)
                     /locus_tag="VF516_20920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110212.1"
                     /translation="VAIGPPPPRETHAVLAGALCQDNLCRCRRDEPGDGGVGVPDTGR
                     KRYEIRLTSPQELWVTINRQAVLYKSPERVEDCFYVDLASGAQELELRASNPDGVSAG
                     LQIHELGTKTRSWYDTFAFRCGVTGVCSLEELEAQKAEYAGKHNLFDRCGTTKVKGLS
                     WDHGKAPDQLHPSELVVRLTLDIYKFAAWKPHGDDTCGMPGQGRPPANEPVPPPGEGP
                     "
BASE COUNT          267 a          798 c          751 g          307 t
ORIGIN      
        1 accaggccga gccagcggcc gatcggcatc agccggtcgg cggtgttgtc ctcgcgcagc
       61 agcaaccgga actcggcgcg cgacgtcatc atccggtacg gctcggacgt gccgcgcgtg
      121 gtcaggtcat cgaccagcac gccgccgtac gcctgatcgc ggcgcaggat caacggctcg
      181 cgatcgccgg ggccgaaacc cagcgccagc gccgcgttga tcccggcgag caacccctgc
      241 accgccgcct cctcgtagcc cgacgtgccg ttgatctggc ccgcgaagta caggccgtgc
      301 acccggcggg tctccagcgt cggcagcacc tcgcgcggat cgatgaagtc gtactcgacc
      361 gcgtagcccg gccgggtcat ctcggcgcgc tccagccccg ggatgctgcg caggaacgcg
      421 agctggacgt cgaacggcag cgaggtcgag atcccgttgg gatagacttc tccggcgccg
      481 cccagcccga cctcgacccc ctcgggctcg aggaagatct ggtggcgctc cttgtccgca
      541 aaccggacga tcttgtcctc gatgctcggg cagtagcgcg gcccgcggcc ggagatctcc
      601 ttgcggtaca gcggcgagcg cggcaggccg tcgcggatga tcgcgtgggt ccgctcggtg
      661 gtgtacgtga tccagcacgg cacctgcggc agcggtgggc ggtcgcggcc gccccaccgg
      721 aacatcggcg gcggatcgtc gcccggctgg agctcgaggc cggcgacgtc gatcgtccgg
      781 cgatcgagcc ggcacggcgt cccggtcttg agccgggcga gcgggaagcc gagcgcgtcg
      841 agcgagtgcg acaggctcac cgccggggcc tcgccggccc ggccgcccgc tgcgcgggcc
      901 tcgccgacga agatcgcgcc gcgcaggaac gtcccggtgg tcaggatcac cgagcgcgcg
      961 cggtagcgca ctcccatcgt cgtggtcacc ccgaccacgc gcggccgccc gctgccgtcg
     1021 tcggtgtgga gctgcgcgac ctcgccctgg cgcagcgcga gccgatcggt gcgctcgagc
     1081 cgcatccgca tctcgtcgcg gtagcgccgc ttgtcggcct gggcgcgcgt cgagcgcacc
     1141 gccggcccct tggacgcgtt gagccggcgg aactggatgc cggtctcgtc gatcacgctc
     1201 gccatctcgc cgccgagcgc gtcgatctcc ttgaccaggt ggcccttggc gaccccgccg
     1261 atcgccgggt tgcacgacat ctgcgcgacc atgtcgaggc tgccggtcag cgccagcacg
     1321 cgcgcgccca tccgcgccgc ggccagcgcg gcctcgcagc cggcgtggcc ggcgccgacg
     1381 acgatcgcgt cgtagctgtc ggggtactcg tagctcatgg ccggcgcgcg cgggtgggga
     1441 ggtgtgccat atccggccgc gatgtcacgg cccctcgccg ggcggcggca ccggctcgtt
     1501 ggccggcggc cggccctgcc ccggcatccc gcaggtgtcg tcgccgtgcg gcttccacgc
     1561 cgcgaacttg tagatgtcca gcgtcagccg gaccaccagc tcgctcgggt gcagctggtc
     1621 cggcgccttg ccgtggtccc acgacagccc cttcaccttg gtcgtgccgc agcggtcgaa
     1681 caggttgtgc ttgccggcgt actccgcctt ctgggcctcg agctcctcga gcgagcacac
     1741 gccggtcacc ccgcagcgga acgcgaacgt gtcgtaccag ctccgggtct tcgtcccgag
     1801 ctcgtggatc tgcagccccg ccgacacccc gtcggggttg gacgcgcgca gctcgagctc
     1861 ctgcgcgccg ctcgccaggt cgacgtagaa gcagtcctcg acgcgctccg ggctcttgta
     1921 cagcaccgcc tggcggttga tcgtcaccca cagctcctgt ggcgacgtca accggatctc
     1981 gtagcgcttg cggccggtgt cggggacccc gaccccgcca tcgccgggct cgtcgcggcg
     2041 gcagcggcag aggttgtcct ggcacagcgc gccggccagc accgcgtggg tctcccgggg
     2101 cggcggcggg ccgatcgcga cct
//
LOCUS       DASVOR010001090         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_361806,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001090 DASVOR010000000
VERSION     DASVOR010001090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361806"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VF516_20925"
     CDS             complement(<1..225)
                     /locus_tag="VF516_20925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110213.1"
                     /translation="MSELPAEVLARRDRLPLKPAPIALTGVLVELRPLELAADGDALH
                     AVSSGAPFSLAGRSVAAYDPEERIWRWMSGG"
     gene            complement(255..1343)
                     /locus_tag="VF516_20930"
     CDS             complement(255..1343)
                     /locus_tag="VF516_20930"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HEX8110214.1"
                     /translation="MTRESNPRIRGLTVRALRVPMAQPHKTAGGTVAESPLVLTDVTT
                     DDGVVGHSLVFTYTPAALKPTADLVRNLESLIAGEPLAPAEIEQKLARRFRLLGTQGL
                     VGMALAAIDMALWDALARRHGVSLVRLLGGAPRPVPAYGAVGFEGVTGSAKVAEDWAR
                     RGFTGVKAKIGYATVAEDVATVRAMREAVGPEVAIMVDYNQCLTPAEAVQRLRVLDDE
                     GLTWVEEPTLAHDHAGHATIAREARTPIQCGENWWGTLDMQHAIEAGASDFMMLDVMK
                     IGGVTGWMRAAALGHARGIRLSSHLWSELSAQLLCVTPTAHWLEYSDWWNAILREPLG
                     IERGMAQVEHAEATGVAWNDKAVERFSI"
     gene            1531..3291
                     /locus_tag="VF516_20935"
     CDS             1531..3291
                     /locus_tag="VF516_20935"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8110215.1"
                     /translation="MRTLLVALLAFAISCAHPAPAPGPANASPAARDRTWIERSNQNA
                     QLLLAVQAQFQPEAAARTGMTGLDDRISDFEPGHQQRQRDAIAKAQAELEKRLAAEQD
                     PLVAQDLQIMIDAARRQIKGSELREKLEVPYYNLPQMIFGSMRSLLDAQIAAERRPAA
                     LVRLRKYLGMDGQTSRIQLAEAETREGMQKWLLAPARLQVENDLDTAAPVLAGIARLF
                     KEYKIEGAEPVLAEMHKQLTAYLEFVRTEVLPKARDDFRLPPEIYAFNLQQVGVDIPP
                     DELARRAHQAFTEIQGEMQKLAAQIAQQHGWPSPDYRDVIKQLKKQQIPDDQVLDFYI
                     HRLGRIEDIIRTHRLVTLPSRPARIRLGTPAENAQQPAPHMVPPRFLGNTGEQGEFVL
                     PLSVPGKNTQKYDDFNFDAAAWTLTAHEARPGHELQFSRQIENGVSVARALFAFNSVN
                     VEGWGLYSEAITLPYMPLEGQLISLQLRLQRAARAFVDPELQIPGKWTPEAARAFLQK
                     EVGLSPAFATEEVDRFTFRSPAQATAYFYGYTKLLELRDAAQKQLGDRFDALQFHDLI
                     LAQGLLPPDLMRKAVLAELH"
     gene            3395..>4013
                     /locus_tag="VF516_20940"
     CDS             3395..>4013
                     /locus_tag="VF516_20940"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX8110216.1"
                     /translation="MAHPGSGLSRLARPTIRGVSTYTVLGFAGYAVASALGAALAIAW
                     RLSLGDRLDALIAPPLAFLAVVHANRRYHGRERIVFYQTASAAVVSAAAVSFVLGGHP
                     ARVADVATAGIGAFLVFGRAGCFSVACCHGRPARHGIAYGPAHVAVGFWSRWSGRTLW
                     PVQLIEAAASGALVAIALAIGWDDPGAPALIYTIAYGCVRFALELV"
BASE COUNT          542 a         1592 c         1334 g          545 t
ORIGIN      
        1 cccgcccgac atccagcgcc agatccgctc ctcggggtcg tacgcggcga ccgagcgccc
       61 ggcgagcgag aacggcgcgc ccgacgacac cgcgtgcagc gcgtcgccgt cggcggcgag
      121 ctcgagcggc cgcagctcga ccagcacgcc ggtgagggcg atcggggccg gcttgagcgg
      181 caggcggtcg cggcgggcga ggacctcggc ggggagctcg gacatcgcgc acatcctacc
      241 gcctgggcgg ctcgtcagat cgagaaccgc tccaccgcct tgtcgttcca cgccacgccg
      301 gtcgcctcgg cgtgctcgac ctgggccatg ccgcgctcga tccccagcgg ctcgcgcagg
      361 atcgcgttcc accagtcgga gtactcgagc cagtgcgcgg tcggcgtcac gcacaggagc
      421 tgcgcgctga gctcggacca caggtggctc gacagccgga tgccccgggc gtggccgagc
      481 gccgccgccc gcatccagcc ggtcaccccg ccgatcttca tcacatcgag catcatgaag
      541 tccgacgcgc cggcctcgat cgcgtgctgc atgtcgagcg tgccccacca gttctcgccg
      601 cactggatcg gcgtgcgcgc ctcgcgcgcg atggttgcgt ggccggcgtg atcgtgcgcc
      661 agcgtcggct cctcgaccca ggtcaggccc tcgtcgtcga gcacgcgcag ccgctgcacc
      721 gcctcggccg gggtcaggca ctggttgtag tccaccatga tcgcgacctc gggcccgacc
      781 gcctcgcgca tcgcgcgcac cgtcgcgacg tcctcggcga ccgtggcgta gccgatcttc
      841 gccttgaccc cggtgaagcc gcggcgcgcc cagtcctcgg ccaccttcgc cgagccggtg
      901 acgccctcga acccgaccgc gccgtacgcc ggcaccggcc gcggcgcacc gccgagcagc
      961 cggaccagcg acacgccgtg gcggcgcgcc agcgcgtccc acagcgccat gtcgatcgcc
     1021 gccagcgcca tgccgaccag gccctgggtg ccgagcagcc ggaaccgccg cgccagcttc
     1081 tgctcgatct ccgccggcgc cagcggctcg cccgcgatca gcgactccag gttgcggacc
     1141 aggtcggccg tcggcttgag cgccgcgggc gtgtaggtga acaccaggct gtggccgacc
     1201 acgccgtcgt cggtcgtgac gtcggtcagc accagcggcg actccgccac cgtgccgccg
     1261 gccgtcttgt gcggctgtgc catcggtacc cggagcgcgc gcaccgtcag gccgcggatc
     1321 cggggattgc tctcgcgtgt catggggcct cctgccaggc cgcgagtata cggccgtcgt
     1381 gacacgggcc gtcacggccc ccggccgccg cgacgcgggg ctgcgacagg tgcgggtcgc
     1441 ggggcgccgc gatgttgccg tccgtgaccc gccgcggcgg cgagggttcg ccattgcagg
     1501 cccaggccgc gcggatacta tgcgcgacct atgcggaccc tcctcgtcgc cttgcttgcc
     1561 ttcgcgatct cgtgcgcgca ccctgcgccc gcgccgggac cggcgaacgc ctcgcccgcc
     1621 gcgcgcgatc gaacctggat cgagcgcagc aaccagaacg cgcagctcct gctcgccgtg
     1681 caggcccagt tccagcccga ggccgccgcg cgcaccggca tgaccggcct cgacgatcgg
     1741 atctccgact tcgagcccgg ccaccagcag cgccagcgcg acgcgatcgc caaggcccag
     1801 gccgagctcg agaagcggct cgccgccgag caggacccgc tggtcgccca ggatctccag
     1861 atcatgatcg acgccgccag gcgccagatc aagggcagcg agctgcgtga gaagctcgag
     1921 gtgccgtact acaacctgcc gcagatgatc ttcggcagca tgcgctcgct gctcgacgca
     1981 cagatcgcgg cggagcgccg gccggccgcg ctggtccggc tgcgcaagta cctcggcatg
     2041 gacggccaga cctcgcggat ccagctcgcc gaggccgaga cccgcgaggg catgcagaag
     2101 tggctgctcg cgccggcccg gctccaggtc gagaacgacc tcgacaccgc cgcgcccgtg
     2161 ctcgccggca tcgcccggct gttcaaggag tacaagatcg agggcgccga gccggtgctc
     2221 gccgagatgc acaagcagct caccgcctac ctcgagttcg tgcgcaccga ggtgctgccc
     2281 aaggcgcgcg acgacttccg cctgccgccc gagatctacg cgttcaacct ccagcaggtc
     2341 ggcgtcgaca tcccgcccga cgagctcgcc cggcgcgccc accaggcgtt caccgagatc
     2401 cagggcgaga tgcagaagct cgcggcgcag atcgcccagc agcacggctg gccgtcgccg
     2461 gattaccgcg acgtgatcaa gcagctcaag aagcagcaga tccccgacga ccaggtgctc
     2521 gacttctaca tccaccggct cggccggatc gaggacatca tccgcaccca ccgcctggtc
     2581 acgctgccca gccgcccggc gcggatccgg ctcggcaccc cggcggagaa cgcccagcag
     2641 cccgcgcccc acatggtgcc gccgcgcttc ctcggcaaca ccggcgagca gggcgagttc
     2701 gtgctgccgc tctcggtgcc cggcaagaac acccagaagt acgacgactt caacttcgac
     2761 gccgcggcgt ggacgctgac cgcccacgag gcgcgccccg gccacgagct gcagttctcg
     2821 cggcagatcg agaacggcgt gtcggtcgcc cgcgcgctgt tcgcgttcaa cagcgtcaac
     2881 gtcgagggct ggggcctgta ctccgaggcg atcacgctgc cgtacatgcc gctcgagggc
     2941 cagctgatca gcctgcagct ccggctgcag cgcgccgcgc gcgcgttcgt cgaccccgag
     3001 ctccagatcc ccggcaagtg gacgccggag gccgcgcgcg cgttcctcca gaaggaggtc
     3061 ggcctgtcgc ccgcgttcgc gaccgaggag gtcgatcgct tcaccttccg ctcgccggcc
     3121 caggccaccg cctacttcta cggctacacc aagctgctcg agctgcgcga cgccgcgcag
     3181 aagcagctcg gcgaccggtt cgacgccctc cagttccacg acctgatcct cgcccagggc
     3241 ctcttgccgc ccgacctgat gcgcaaggcc gtgctcgccg agctgcactg acccgacgcc
     3301 ccggagcgcc gtcggcgtca cgccgcggct ttggcccgcg ccgggcggtc gctgccgatc
     3361 ccgaccgcac gcgatcgtgc gaagttcgca gcccgtggcg caccccggtt ccggcctgtc
     3421 tcgcctcgcc cgcccgacga tccgaggcgt cagcacctac accgtgctgg gcttcgccgg
     3481 ctacgccgtc gcctcggcgc tcggcgccgc cctcgcgatc gcctggcggc tctcgctggg
     3541 cgaccggctc gacgcgctga tcgccccgcc gctcgccttc ctcgccgtcg tccacgccaa
     3601 ccgccgctac cacggccgcg agcgcatcgt gttctaccag accgcctcgg ccgccgtcgt
     3661 gtcggccgcc gcggtcagct tcgtgctcgg cggccacccc gcgcgcgtcg ccgacgttgc
     3721 caccgccggc atcggcgcgt tcctcgtgtt cggccgtgcc ggctgcttct ccgtcgcgtg
     3781 ctgccacggc cgcccggcgc gccatggcat cgcctacggc cccgcacacg tcgccgtcgg
     3841 cttctggtcg cgctggtccg gccgcacgct gtggccggtc cagctcatcg aggccgcggc
     3901 gagcggcgcg ctcgtcgcga tcgccctcgc catcggctgg gacgatcctg gcgcaccggc
     3961 gctgatctat accatcgcgt acggctgcgt gcggttcgcg ctggagctcg tgc
//
LOCUS       DASVOR010001091         2523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_273370,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001091 DASVOR010000000
VERSION     DASVOR010001091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273370"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..924)
                     /locus_tag="VF516_20945"
     CDS             complement(<1..924)
                     /locus_tag="VF516_20945"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110217.1"
                     /translation="MVGTLLEGKYRVDARVAAGGFGVVYAGHHIGLARPLAIKVLRCG
                     PELGVAERGQMIAQFLAEARLVARLRHPAVVRVSDAGITVTERYPEGVPWIVMEWLDG
                     ETLARDLARRRVRGELGRTRDETMALLRPVVEAIAEAHDAGIVHRDLTPNNLMLVPGR
                     NSPPVRVLDFGIAKVMHPEAAVPSGSTTTDSAVRAFCPAYAAPEQLTGRRTGPWTDVH
                     ALGLLFTEVLCGWRAPAVDDLEAHYRAALDPTRPTPATLGVDAGNWEAILARALALNP
                     RDRYASAGELLAALDGAPRAEPVRPVSAPGPG"
     gene            complement(1236..2165)
                     /locus_tag="VF516_20950"
     CDS             complement(1236..2165)
                     /locus_tag="VF516_20950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110218.1"
                     /translation="MRPAIPSAMFSALWAGLAACGAMTSTSTHIGGPCDASNKCDAGQ
                     ICDRTNPGGQVCVDANGDMDGDGIPNGKDFCQHMAGGANDEDGDGIGDECDACPIAPP
                     PVSAEGDGDAVTAPCDPDTRTPGDRILLFNGFNAAVAGASSAWKFQNGEAIVTPSAPD
                     MPEELPFPLATASNHLTIFAAYRIDGVSTTAATADAAVRTRANLPFDKTMVQCGGSRA
                     QGADAVLLTQTDTDMAMINSSKPVSTAFSPTATYRIAQQLDGAVANCALVGDTMANQG
                     AIQLAINGSTPSQAVLYARGATVRFSYILVVGQ"
     gene            complement(2320..>2523)
                     /locus_tag="VF516_20955"
     CDS             complement(2320..>2523)
                     /locus_tag="VF516_20955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-pyrroline-5-carboxylate dehydrogenase"
                     /protein_id="HEX8110219.1"
                     /translation="DAAGNFYINDKPTGAVVGQQPFGGARASGTDDKAGSALNLLRFV
                     AARTIKETFAPPTDWRYPFLDQP"
BASE COUNT          318 a          916 c          921 g          368 t
ORIGIN      
        1 ccctggcccc ggggcgctga ccgggcgcac cggctcggcc cgcggcgcac cgtcgagggc
       61 ggcgagcagc tcgccggccg acgcgtaccg atcgcgcggg ttcagggcca gggcgcgcgc
      121 caggatcgcc tcccagttgc ccgcgtccac gcccagcgtc gccggcgtgg gtcgcgtcgg
      181 gtcgagcgcc gcgcggtaat gcgcctcgag atcgtcgacc gccggcgcgc gccatccgca
      241 cagcacctcc gtgaacagca gccccagcgc gtgcacgtcc gtccacggcc cggtacgccg
      301 gccggtcagc tgctccggcg cggcgtacgc ggggcagaac gcccggaccg ccgagtcggt
      361 cgtggtgctg ccgctcggca ccgccgcctc cgggtgcatc accttcgcga tgccgaagtc
      421 gaggacgcgg accggcggcg agttccggcc cgggacgagc atcaggttgt tcggcgtcag
      481 gtcgcggtgg acgatcccgg cgtcgtgcgc ctcggcgatt gcctcgacga ccgggcgcag
      541 cagcgccatc gtctcgtccc gcgtccgccc cagctcaccg cgcacccggc gccgcgcgag
      601 gtcgcgcgcc agggtctcgc cgtcgagcca ctccatcacg atccagggaa ctccctcggg
      661 gtatcgctcg gtcaccgtga tcccggcgtc gctgacgcgg accaccgccg ggtggcgaag
      721 ccgggcgacg agcctggcct ccgcgaggaa ctgcgcgatc atctggccgc gctcggcgac
      781 gccgagctcc ggtccgcatc ggaggacctt gatcgcgagc ggcctggcga gcccgatgtg
      841 gtgccctgcg tagaccacgc cgaagccacc cgcggccacg cgcgcgtcga cccggtactt
      901 tccctcgagc agcgtcccga ccataccgag cgaatcatca ccgccccgca cggtgacaac
      961 gccgctgtct tcgcccgcca atgcgctgtc ttcgcccgcc aatgcgccgt ccaagatccc
     1021 ccccgtttcc gtgcgcaatc gtatcgtgat ccagcggcgg ttgccacgtc gaatgtcgcg
     1081 cgcggcgcgg cgcggcgcgg accgacccga gggcaggtag gggaggacgt ggggggctgg
     1141 aggcaccagg accaccgcaa gagcatcctc gctctgggtc gcggtcatgc ttgccgagca
     1201 ggcagctcgg cgccgcggcg gatcgggcgg ctagctcact ggccgacgac caggatgtac
     1261 gagaaccgga cggtcgcgcc gcgcgcgtag agcaccgcct gggatggcgt gctgccgttg
     1321 atcgccagct ggatcgcgcc ctggttggcc atcgtgtctc cgaccagcgc gcagttggcg
     1381 accgcgccgt cgagttgctg ggcgatccgg tacgtggcgg tcgggctgaa cgccgtgctg
     1441 acgggcttgg agctgttgat catggccatg tccgtgtcgg tctgggtgag cagcaccgcg
     1501 tcggcgccct gggcgcgcga gcccccgcac tggaccatcg tcttgtcgaa cggaaggttc
     1561 gcccgggtcc tcaccgcggc gtccgcggtg gccgcggtcg tggacacccc gtcgatccgg
     1621 tatgcggcga agatcgtcag gtggttggac gcggtggcga gcggaaacgg cagctcctcc
     1681 ggcatgtccg gggcgctggg cgtcacgatc gcctcgccgt tctggaactt ccacgcggag
     1741 ctcgcgccgg cgaccgcggc gttgaagccg ttgaacagca ggatccggtc gcccggcgtg
     1801 cgggtgtcgg gatcgcacgg ggcggtgacc gcgtcgccgt cgccctcggc gctcaccggc
     1861 ggcggcgcga tcgggcaggc gtcgcactcg tcgccgatgc cgtcgccgtc ctcgtcattg
     1921 gcgccgccgg ccatgtgctg gcagaagtcc ttgccgttgg ggatgccgtc gccgtccatg
     1981 tcgccattgg cgtcgacgca cacctgcccg cccggattgg tgcgatcgca gatctggccg
     2041 gcgtcgcact tgttgctggc gtcgcacggt ccgccgatgt gggtcgaggt cgaggtcatc
     2101 gcgccgcagg cggccaggcc ggcccacagc gccgagaaca tcgcggacgg gatcgcaggg
     2161 cgcacgccgc gagcgtatca ccgcgccggc gccggcgacc gcgccatcgc cggctgccag
     2221 catgcgctgg tcgatgtcag cggcgatcgt gtgacgtgcg ccgcgttgcg acccgccggc
     2281 ccgagtctct tgcgcggcgg atcacggtcg cctcggcgat cacggctgat cgaggaacgg
     2341 atagcgccag tcggtcggcg gcgcgaacgt ctccttgatc gtgcgggcgg cgacgaaccg
     2401 cagcaggttg agcgccgagc cggccttgtc gtcggtgccc gacgcgcgcg cgccgccgaa
     2461 cggctgctgg ccgaccaccg cgccggtcgg cttgtcgttg atgtagaagt tgccggccgc
     2521 gtc
//
LOCUS       DASVOR010001092         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390649,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001092 DASVOR010000000
VERSION     DASVOR010001092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390649"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VF516_20960"
     CDS             complement(<1..131)
                     /locus_tag="VF516_20960"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8110220.1"
                     /translation="MRTVYGARMTKPFADRTVVITGASAGIGRACARQFAEAGARVVL
                     "
     gene            complement(166..>2062)
                     /locus_tag="VF516_20965"
     CDS             complement(166..>2062)
                     /locus_tag="VF516_20965"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX8110221.1"
                     /translation="SFGTVGTGTTTSTMSCTVTNNGEAPSGALSIVLAGSDPGQFSLA
                     APSSGDCMPGTTQLAGGSTCTVRVAFDPTTTGAKSARVDVSGTPGGSGSCTLSGTGVD
                     PAALSPATTSNAFDSVQLGQSGSAFTWTITNTGGVPTGTLTRSTSGDTADFTITDSGT
                     EPCTGTLGAGATCSVLITFSPASFANKSLSITLSASPGGMARFTATGSALGTLVLSKA
                     GGGTGTVVSTPAGISCLGGCSTQTAQFSGLSVVTLEAKGLPGSYFQGWAGACSGTRRF
                     CTLSIDQATQTVTANFVPNTQNLVFATSETYAANFGGLAAADANCARLANAAGLPGTY
                     VAWLSTSTVNAKDRLGSARGWVRLDGEPFADTVAALTTNHAVLHPIVYDENGTALAFG
                     SDVAWTGTDVNGANPLACSDWTSTSASAQGWIGEAGAGPLGWTRINPNACSQGPYRLY
                     CFQTDKTAPVTITPVAGKKIWVTSNDLPPDMTIAAANNACNTQRPAGVASGIALLATT
                     MTAPSTLVGASTNYVRPDGVLVANGADLAATSNNLRPLRSGIWQHGDGTYDVTSSDAW
                     TGANSFGSFSQTSTSGFDCNDWTAVSSLVRGGFGIFQRTSYSFWNQGSLQCDSLALLF
                     RVYCVEP"
BASE COUNT          290 a          688 c          769 g          315 t
ORIGIN      
        1 agcacgacgc gggcgccggc ctcggcgaac tggcgcgcgc acgcccggcc gatcccggcg
       61 gacgcgccgg tgatcaccac ggttcgatcg gcgaatggtt tcgtcatgcg cgcaccgtac
      121 acggtccgca tcggtgctga aagtccgccg cggactgccc gttcatcacg gctcgacgca
      181 gtacacccgg aagaggaggg ccaggctgtc gcattgcagc gagccctggt tccagaagct
      241 gtagctggtg cgctggaaga tgccgaagcc gccgcgcacc agggacgaca ccgctgtcca
      301 gtcgttgcag tcaaagccgc tggtcgacgt ctggctgaac gagccgaagg agttggcgcc
      361 ggtccacgcg tcggacgagg tcacgtcgta ggtgccgtcg ccgtgctgcc agatgccgga
      421 ccgcagtgga cggaggttgt tgctggtcgc cgccaggtcg gcgccgttgg cgaccagcac
      481 gccgtcgggg cggacgtagt tggtggatgc gccgaccagg gtcgacggcg cggtcatggt
      541 cgtcgccagg agcgcgatgc cgctcgccac gccggcgggc cgctgcgtgt tgcaggcgtt
      601 gttggcggcc gcgatcgtca tgtcgggcgg gaggtcgttg ctggtgaccc agatcttctt
      661 gccggccacc ggcgtgatcg tgaccggcgc ggtcttgtcg gtctggaagc agtagaggcg
      721 gtatgggccc tggctgcacg cgttcggatt gatccgtgtc cagccgagcg ggccggcgcc
      781 ggcctcgccg atccagcctt gcgcggacgc cgacgtggag gtccagtccg agcacgcgag
      841 cgggttggcg ccattgacgt cggtaccggt ccacgccaca tcgctgccga acgccagcgc
      901 ggtgccgttc tcgtcataca cgatcgggtg cagcaccgcg tgattggtgg tgagggccgc
      961 gaccgtatcg gcgaacggct cgccgtcgag gcgaacccag ccgcgcgcgc tgccgaggcg
     1021 atccttggcg ttgaccgtgg aggtcgacag ccacgccaca taggtgccgg gcaggccggc
     1081 cgcgttggcc agccgcgcgc agttggcatc ggccgcggcc agcccaccga agttggcggc
     1141 atacgtctcg gacgtcgcga acacgaggtt ctgggtgttc ggcacgaagt tcgcggtcac
     1201 cgtctgcgtc gcctggtcga tgctcagggt gcagaagcgg cgggtgcccg agcacgcacc
     1261 ggcccagccc tggaagtacg agcccggcag gcccttggcc tcgagcgtga cgaccgacag
     1321 gcccgagaac tgcgcggtct gcgtcgagca tccgcccagg cagctgatac cggctggcgt
     1381 cgacaccacg gtgccggtgc caccgcccgc cttgctgagc accagcgtgc cgagcgcgga
     1441 gccggtcgcc gtgaaccgag ccatgccgcc cgggctcgcc gagagcgtga tgctcaggct
     1501 cttgttggcg aacgacgccg ggctgaacgt gatcaggacc gagcaggtcg cgccggctcc
     1561 cagcgtgccg gtgcacggct ccgtgcccga atccgtgatc gtgaagtcgg cggtgtcgcc
     1621 gctggtcgag cgcgtgaggg tgccggtcgg cacgccgccg gtgttggtga tcgtccacgt
     1681 gaaggccgag ccgctctggc cgagctgcac gctgtcgaac gcgttgctcg tggtcgcagg
     1741 cgacagcgcg gccgggtcga cgccggtgcc gctcagcgtg cagctcccac taccgccggg
     1801 tgtgcccgag acgtcgaccc gcgcgctctt ggcgccggtc gtggtcggat cgaacgcgac
     1861 ccgcacggtg caggtcgaac cgcccgcgag ctgcgtcgtg ccgggcatgc agtcgccgct
     1921 ggacggcgcc gcgagcgaga actgccctgg atcgctgccg gccaggacga tcgacagcgc
     1981 gccgctcggc gcctcgccgt tgttcgtgac cgtacaggac atggtcgagg tggtggtgcc
     2041 ggtgccgacg gtgccgaacg ag
//
LOCUS       DASVOR010001093         6085 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_478700,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001093 DASVOR010000000
VERSION     DASVOR010001093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478700"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="VF516_20970"
     CDS             <1..384
                     /locus_tag="VF516_20970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110222.1"
                     /translation="ATLIAPIGSGGANRTRGTLVRLAASRAFADQRGEIEADAMVERF
                     TDVDASTGMCMTSLDTFACYGASKTVAAQAGALATWRIGREWLLLADAHAGYRDVRSA
                     SIAGPIVWPKVVSITSFIRVQWRYR"
     gene            381..3965
                     /locus_tag="VF516_20975"
     CDS             381..3965
                     /locus_tag="VF516_20975"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HEX8110223.1"
                     /translation="MNLRIAAIAIAVIAAGCDLGRLDTPTGPTTIAGPSSGCSGACHG
                     EGDQIAPPRDTAGRTDLAAIGVGAHAQHLGASTWHKRFACETCHVVPQQVGDPGHIFN
                     PGPSGPVQDKLPAEVVFTGLGTGASWDHQTATCTSSYCHGDTLHQIDPATNATVKGAG
                     GAITRPVWTQVDGTQSKCGACHGTPPPAPHPQNTDCGLCHPSMNPGDFAAGKISYPEL
                     HIDGVVEVNSTQACDSCHGSGGNPAPPRDAHGNTQTAAPGVGAHAQHMTTTSSWHAPI
                     ACNECHLVPGSSTDQTHIDQIDEVTLDPTVQVPGSLLPGGGTGGKLQVPGAAWNPSAL
                     TCAGTYCHGGGKSPLTGGTAISPKWTQVDGTQSQCQSCHGMPPPAPHPVDSNCGKCHP
                     TMTPGNNTTITYPARHIDGNVDVISDQPCDSCHGSGGNAAPPSDTTGGTSNGLRGVGA
                     HRSHLSASTWHNQITCDQCHKVPTSLLSIGHIDSPLPAEVMFGNLAGQTAAWNGSTCS
                     NVYCHGDTLKDGGTPAGGTATRPVWTVVNGSQSQCGSCHGTPPPPPHPVDSDCGKCHP
                     TMTPGGGLVITDPTRHIDGNLDVNTDQACNACHGGVNNAPPRDTQGNTATTARGVGAH
                     QSHLGPANPFFKPIACSDCHRVPATVSSVGHIDTPLPAEVIFSGRAGTSPIWNGAQCS
                     NVYCHGATLTDGGGGAGGTATSPVWQKVDGSQAQCTSCHGFPPPAPHPADSDCGKCHL
                     DVKPGASTTFLDTTRHIDGNLDVNTSQACNSCHGGTSNAPPRDTTGGTATTLRGVGAH
                     QVHLTAGSTWHLDVTCNECHRVPATVSSVGHIDTPLPAEVIFSGLGAGGTWNGANCAS
                     YCHGSTLTTSAGTPAGGTATSPAWTQVDGTQSQCTSCHGAPPPAPHPQDTACQNCHAD
                     AGPSLTIKTPAQHIDGTVQITQVHPAGYNDRAKHGYDFDAQGPSSCATAGCHGTALTG
                     GNTGGPSCNTCHTASWQTDCKFCHGTTADGAPPQGVLGQTAASDPHVGAHARHVGATT
                     THAAWDCTYCHTRPSTALTPGHIDGTGGIVQAEVVFSSLNPSSAYSFTASTCATSYCH
                     GSGVTTRTSPAWTSTTALACVDGCHGGDPNRTGMSSGHRRSDHKKPCATCHKSVVDAN
                     NNIIAAGLHVNGAKDVQFSAAGSSYNATNKSCTGTGNGCHGAGTKTGW"
     gene            4455..5792
                     /locus_tag="VF516_20980"
     CDS             4455..5792
                     /locus_tag="VF516_20980"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8110224.1"
                     /translation="MKAHQLYKLPLLLPASLLFACSSPSGTQPSDPDNDPVVAAATQE
                     DDSAPGPHVNAVTEAPTGFDGLPNGNCTAAEFRATGLTFSEVEAFEDGIGPTFNNTSC
                     ISCHESPRGTSEANDTNTTNNKVFGGSGSQITELRAGHFNGISFVDHPGGSLINDRAN
                     DRSIQEHILSGNEVQTLRLTLSIAGDGFVEAINSNTLVAIANAQPAAQRGTVIQVPVL
                     EAAGQIRVGRFGWKNQNSSLLSFAGDAYVNEMGVTNRLFQTENTSNGTNVQGGTFDGK
                     GDPAGTGEDDAGDIDEFTTFMRCLKAPPRGPITAAVTAGQSTFNAIGCAVCHVGTITT
                     SPAGMLINGGALTVSTALGDKNIHPFSDYLLHDIGTGDGIVQNGGQGTRNQIRTAPLW
                     GIGARTRFMHDGASLTVSDAIARHGNQGATARANFNALSATDQSNVRAFIFSL"
BASE COUNT         1054 a         2297 c         1993 g          741 t
ORIGIN      
        1 gccacgctga tcgcgccgat cggcagcggc ggggcgaacc gtacgcgcgg caccctggtc
       61 cggctcgcgg cgagccgtgc gttcgccgac cagcgcggcg agatcgaggc cgacgcgatg
      121 gtcgagcggt tcaccgacgt cgatgcgagc accgggatgt gcatgacctc gctcgacacc
      181 ttcgcgtgct acggcgcgtc gaagaccgtg gccgcccagg ccggcgcgct cgcgacctgg
      241 cggatcggcc gcgagtggct gctgctcgcc gacgcccacg ccggctatcg cgacgtccgc
      301 tcggcgtcga tcgcgggccc gatcgtgtgg cccaaggtcg tctcgatcac gtcgttcatt
      361 cgcgtccagt ggaggtaccg atgaaccttc gaatcgccgc gatcgccatc gcggtcatcg
      421 ccgccggctg cgatctcggc cggctcgaca caccgaccgg cccgacgacc atcgccgggc
      481 catcgagcgg ctgctcgggc gcgtgccacg gcgagggcga tcagatcgcg ccgccgcgcg
      541 acaccgccgg gcgcaccgat ctggcggcga tcggcgtcgg cgcacacgcc cagcacctcg
      601 gggcgtcgac ctggcacaag cggttcgcgt gcgagacctg ccacgtcgtg ccgcagcagg
      661 tcggcgaccc cggccacatc ttcaaccccg gccccagcgg cccggtccag gataagctgc
      721 ccgccgaggt cgtcttcacc ggcctgggca ccggcgcgag ctgggatcac cagaccgcca
      781 cctgcaccag cagctactgc cacggcgaca cgctgcacca gatcgacccg gcgaccaacg
      841 cgacggtcaa gggcgccggc ggcgcgatca cccggccggt gtggacccag gtcgacggca
      901 cgcagagcaa gtgcggcgcc tgccacggca cgccgccgcc ggcgccgcac ccgcagaaca
      961 ccgactgcgg gctgtgccat ccgtcgatga accccggcga cttcgcggcc ggcaagatct
     1021 cgtatcccga gctccacatc gacggcgtgg tcgaggtcaa ctcgacccag gcgtgcgaca
     1081 gctgccacgg ctcgggcggc aaccccgcgc cgccgcgcga cgcgcacggc aacacgcaga
     1141 ccgcggcgcc cggcgtcggc gcccacgccc agcacatgac cacgacgtcg agctggcacg
     1201 cgccgatcgc gtgcaacgag tgccacctcg tcccgggcag ctcgaccgat cagacccaca
     1261 tcgaccagat cgacgaggtc accctcgatc cgaccgtgca ggtgccgggt tcgctgctgc
     1321 ccggcggcgg caccggcggc aagctccagg tccccggggc ggcatggaat ccgtcggcgc
     1381 tgacctgcgc ggggacctat tgccacggcg gcggcaagag cccgctgacc ggcggcaccg
     1441 cgatctcgcc gaagtggacc caggtcgacg gcacgcagag ccagtgccag tcgtgtcacg
     1501 gcatgccgcc gccggccccg cacccggtcg acagcaactg cggcaagtgc cacccgacga
     1561 tgacgccggg caacaacacg acgatcacgt accccgccag gcacatcgac ggcaacgtcg
     1621 acgtgatcag cgatcagccg tgcgactcgt gccacggctc gggcggcaac gccgcgccgc
     1681 ccagcgacac cacgggcggc acgtccaacg gcctgcgcgg ggtcggcgcg caccgcagcc
     1741 acctgagcgc gtcgacctgg cacaaccaga tcacctgcga tcagtgccac aaggtcccga
     1801 cctcgctgct gtcgatcggc cacatcgact cgccgctgcc cgccgaggtg atgttcggca
     1861 acctcgccgg acagaccgcg gcgtggaacg gctcgacgtg cagcaacgtg tactgccacg
     1921 gcgatacgct caaggacggc ggcacgccgg cgggcgggac cgcgacccgg ccggtgtgga
     1981 cggtggtcaa cggctcgcag agccagtgcg gctcgtgcca cggcacgccg ccgccgccgc
     2041 cgcatccggt cgacagcgac tgcggcaagt gccacccgac gatgacgccg ggcggcggcc
     2101 tcgtgatcac cgatccgacc cgccacatcg acggcaacct cgacgtcaac accgaccagg
     2161 cgtgcaacgc gtgccacggc ggcgtcaaca acgcgccgcc cagggacacc cagggcaaca
     2221 ccgcgaccac cgcgcgcggc gtcggcgcgc accagagcca cctcggcccg gccaacccgt
     2281 tcttcaagcc gatcgcgtgc agcgactgcc accgggtccc cgcgacggtg agctcggtcg
     2341 gccacatcga caccccgctg cccgccgagg tgatcttcag cggccgcgcc gggacgtcgc
     2401 cgatctggaa cggcgcgcag tgctcgaacg tgtactgcca cggcgcgacc ctgaccgacg
     2461 gcggcggcgg cgcgggcggc acggcgacct cgccggtgtg gcagaaggtc gacggctcgc
     2521 aggcccagtg cacgtcgtgc cacggcttcc cgccgccggc gccgcacccg gcggacagcg
     2581 actgcggcaa gtgccacctg gacgtcaagc cgggcgccag cacgaccttc ctcgacacca
     2641 ccaggcacat cgacggcaac ctcgacgtca acaccagcca ggcgtgcaac tcgtgccacg
     2701 gcggcaccag caacgcgccg cccagggaca ccacgggcgg caccgcgacg acgctgcgcg
     2761 gcgtcggcgc ccaccaggtc cacttgacgg ccggctcgac ctggcacctc gacgtcacct
     2821 gcaacgagtg ccaccgggtc cccgcgacgg tgagctcggt cggtcacatc gacacgccgc
     2881 tgcccgccga ggtgatcttc agcgggctcg gcgccggcgg cacgtggaac ggcgcgaact
     2941 gcgcgagcta ctgccacggc tcgacgctca cgacctcggc cggcacgccg gccggcggca
     3001 ccgcgacctc gccggcgtgg acccaggtcg acggcacgca gagccagtgc acgtcgtgcc
     3061 acggcgcgcc accgccggcg ccgcacccgc aggacaccgc gtgccagaac tgccacgccg
     3121 acgccggccc cagcctgacg atcaagaccc cggcgcagca catcgacggc acggtgcaga
     3181 tcacacaggt ccaccccgcc ggttacaacg accgcgccaa gcacggctac gacttcgacg
     3241 cccagggccc gtcgtcgtgc gcgaccgcgg gctgccacgg caccgcgctg accggcggca
     3301 acaccggcgg cccgagctgc aacacctgcc acaccgccag ctggcagacc gactgcaagt
     3361 tctgccacgg caccaccgcc gacggcgcgc caccccaggg cgtgctcggc cagaccgccg
     3421 cgagcgatcc gcacgtcggc gcccacgcca ggcacgtcgg cgcgaccacg acccacgcgg
     3481 cgtgggactg cacgtactgc cacaccaggc catcgaccgc gctgaccccg ggccacatcg
     3541 acgggaccgg cggcatcgtg caggccgagg tcgtgttctc gtcgctcaac ccgtcgagcg
     3601 cctacagctt caccgccagc acctgcgcca ccagctactg ccacggcagc ggcgtcacga
     3661 ccaggacctc gccggcgtgg acctcgacga ccgcgctggc ctgcgtcgac ggctgccacg
     3721 gcggcgatcc caaccgcacc gggatgtcgt cgggccaccg ccgcagcgac cacaagaagc
     3781 cgtgcgcgac ctgccacaag tccgtggtcg acgcgaacaa caacatcatc gccgccgggc
     3841 ttcacgtcaa cggcgccaag gacgtccagt tctccgccgc cgggtcgagc tacaacgcga
     3901 cgaacaaatc gtgcaccggg acggggaacg gctgtcacgg cgccgggacc aagaccggct
     3961 ggtagcgagc gcggccggca agcgacgcct cacgcgacgg cgtcacgcgt caattcggtc
     4021 gcaaaaaact gagatccacg gcgcggggga tgatcaatat tccgcgacca tcgtcgcggc
     4081 gcgtgcgtcg ctgcgggtga cgcgtatcga gatcgagagt cgctaactct gtcgatcacg
     4141 gggaaggcgt gtgacagcgc cgtttagcgg cgcccgacca ggtcgacgac ggaaaatcga
     4201 gttcattggt ccgccagagg cgtgccgcat aagcctttgt caacgcccca atattacgat
     4261 tgacagaggg tgatacccct actgtaaggt ggaaaccgtc agaggggttc ggagaggcgg
     4321 aaggcgatgt cgttcggctt cgaggacccg atgtaggggc ttggagcagc gcgtcttcgt
     4381 accggttcgc tcgtggtgtc cgtgagttct acctgcccag tgctcaccgt tcttaacccc
     4441 aggagaacag agtcatgaaa gcgcatcagc tgtacaaact cccattgcta ttaccggcgt
     4501 cattgctctt cgcctgctca tcaccgagcg gaacccagcc gtcggatccg gataacgacc
     4561 cggtcgtcgc ggcggcgacc caggaggacg atagcgcccc cggcccccac gtcaacgcgg
     4621 tcaccgaggc accgactggc ttcgacggcc tccccaacgg gaactgcacc gcggccgagt
     4681 tcagagcgac gggcctcacg ttctcggagg tcgaggcgtt cgaggacggc atcggcccca
     4741 cgttcaacaa cacctcgtgc atcagctgcc acgagagccc gcgcgggacc tcggaggcga
     4801 acgacaccaa caccaccaac aacaaggtct ttggcggcag cggtagccag atcaccgagc
     4861 ttcgcgccgg ccacttcaac ggcatcagct tcgtcgacca cccgggcggc tcgctgatca
     4921 acgaccgcgc caacgaccgc tcgatccagg agcacatcct gtcgggcaac gaggtccaga
     4981 cgctccggct cacgctcagc atcgcgggcg atggcttcgt cgaggcgatc aacagcaaca
     5041 cgctggtggc gatcgccaac gcccagccgg ccgcgcagcg cggcacggtg atccaggtgc
     5101 cggtgctcga ggcggcgggc cagatccgcg tcggccggtt cggctggaag aaccagaact
     5161 cgagcctcct gtcgttcgcc ggtgatgcct acgtcaacga gatgggcgtc accaaccggc
     5221 tgttccagac cgagaacacg tccaacggga ccaacgtcca gggtggcacg ttcgacggca
     5281 agggggatcc ggcgggtacc ggcgaggacg acgcgggcga catcgacgag ttcacgacgt
     5341 tcatgcgctg cctgaaggcg ccgccgcgcg gaccgatcac cgcggcggtg acggccggcc
     5401 agtcgacgtt caacgccatc ggctgcgcgg tctgccacgt cgggaccatc accacgtccc
     5461 cggcgggcat gctgatcaac ggcggcgcgc tcaccgtgtc gaccgcgctc ggtgacaaga
     5521 acatccaccc gttcagtgac tacctgctgc acgacatcgg caccggtgac ggcatcgtgc
     5581 agaacggcgg ccagggcacg cgcaaccaga tccgcacggc gccgctgtgg ggcatcgggg
     5641 cgcgcacccg gttcatgcac gacggcgcga gcctgacggt gtcggacgcg atcgcgcgcc
     5701 acggcaacca gggcgcgact gcgagggcca acttcaacgc gctgtccgcg accgatcagt
     5761 ccaacgtgcg ggcgttcatc ttctcgctgt agtcgccgtc gccgagggca tgcgcgccct
     5821 ccccgacgcc gcctcacatg agacgccggc cttcctcccc gccatcgggg aggaagcgcc
     5881 ggtgcggatg gagaagcacg gccttccgcg tcgtccgcga aggacaccgc gaacgccgcg
     5941 gaaccgtcat cgttccttac gagcacctgg tgtcggcttg aggccgtgct tctccatccg
     6001 gtagctcagc gtccgccgct agatcccgag ctgcttggcg gcctcggtct ggctgccgcc
     6061 gcacgcctcg agcaccgcgg cgatc
//
LOCUS       DASVOR010001094         2247 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390672,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001094 DASVOR010000000
VERSION     DASVOR010001094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390672"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /locus_tag="VF516_20985"
     CDS             <1..564
                     /locus_tag="VF516_20985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110225.1"
                     /translation="RQVIAAAVAVPVILLAVAGVALVRISRAEREASQQRDELRIEKA
                     NAERAAREAQEARLAEAAERAKKNTKGPVTHSGTIYAAISPAGDEDVLPFMNPSASTV
                     TMKFDTWSQARGVGFPCDTDADTVLYIRNAIGTLLVASNDRDPGHDFCSTATVTVAAG
                     ETVYVHVRAFNADAVIPGYLLVATYLP"
     gene            complement(610..1026)
                     /locus_tag="VF516_20990"
     CDS             complement(610..1026)
                     /locus_tag="VF516_20990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110226.1"
                     /translation="MQVPDAWPNTFRQSRLLSAVDYVQADRLRRKVAEEMARVFSTVD
                     VLLVPSLRDEMLTITNATGHPSLTLRAGFVEVGEARSDWAPDPAHPMPKFSPPRRVPH
                     GVTLIGRLFDEGTLGRAGIALEKALNVAAERPPGFA"
     gene            complement(1081..>2247)
                     /locus_tag="VF516_20995"
     CDS             complement(1081..>2247)
                     /locus_tag="VF516_20995"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8110227.1"
                     /translation="TIAEAEKLMQVSYTPAERAQAAGNWRVSMAPLYERRTGPRKVAL
                     DPTVSPASHWNPALPGLTVGPARNRFVRSPGPPGKLPAKDDDIAFAPVTQLARWIESR
                     QLTSDRLTRIYLERLQRFQPKINAVITLTRDLALAQAKQADAEIAAGKYRGPLHGIPW
                     GAKDLVDTAGIPTTWGAEPFRNRVPRTDAVTVARLHQAGAVLVAKLSLGALALNDIWY
                     GGQTNNPWLLEEGASGSSAGPGSATAAGCVGFALGSETGGSIIAPAMRCGVTGLRPTF
                     GRVARTGAMTLCWSLDKLGPMARTVEDTFLALAALSGPDAGDVSSVPSKLEFDATAGI
                     KGLKVGFFPRWMGEAPATEVDRAALETLKKLGAQPVEVQIPDWPYSSIQVILFA"
BASE COUNT          308 a          762 c          855 g          322 t
ORIGIN      
        1 cgtcaggtca tcgcggcggc cgtcgcggtc ccggtgatcc tgctggcggt ggcgggcgtg
       61 gcgctggtcc ggatcagccg cgccgagcgc gaggcgagcc agcagcgcga cgagctacgc
      121 atcgagaagg ccaacgccga gcgagcggcg cgagaagccc aggaggcgcg cctggccgag
      181 gcagccgagc gcgccaagaa gaacacgaaa ggcccggtca ctcattctgg cacgatctac
      241 gccgcaatat cgccggccgg agacgaggat gtcctcccgt tcatgaaccc gagcgcgagc
      301 accgtgacga tgaagttcga tacctggagc caggcccgtg gggtcggttt tccatgtgac
      361 accgacgccg acacggttct gtacatccgc aacgcgatcg ggacgctgct ggttgccagc
      421 aatgaccgtg acccaggcca cgacttctgc tcgacggcga ccgtcacggt agcggccggc
      481 gagaccgtct atgtccacgt ccgggcgttc aacgcggacg ctgtgatccc gggctacctg
      541 ctcgtggcga cgtacctccc gtgactcacg cggcctggtc tcgcccgacg tcgtggtgaa
      601 tcagcgggcc tacgcgaagc cgggcgggcg ctccgcggcc acgttgagcg ccttctcgag
      661 cgcgatgccg gcgcggccga gcgtgccctc gtcgaacagc cggccgatca gcgtcacgcc
      721 gtgcgggacg cggcggggcg gcgagaactt cggcatcggg tgcgcggggt cgggcgccca
      781 gtcgctgcgg gcctcgccga cctcgacgaa gccggcgcgc agggtcagcg acgggtggcc
      841 ggtggcgttg gtgatggtca gcatctcgtc gcgcagcgac ggcacgagca ggacgtcgac
      901 ggtgctgaac acgcgcgcca tctcctcggc gaccttgcgg cgcagccggt cggcctggac
      961 gtagtcgacc gcggatagca ggcgcgactg gcggaaggtg ttgggccacg cgtcgggcac
     1021 ctgcatcttg agctgatcga ggccgtggct cagggtgagc tcctcgaacg acgcggccgc
     1081 ctaggcgaac aggatcacct ggatcgagct gtacggccag tcggggatct ggacctcgac
     1141 cggctgcgcg ccgagcttct tcagggtctc gagcgcggcg cggtcgacct cggtcgcggg
     1201 ggcctcgccc atccagcgcg ggaagaagcc gaccttgagg cccttgatgc cggcggtggc
     1261 gtcgaactcg agcttgctgg gcacgctcga gacgtcgccc gcgtcggggc cgctcagcgc
     1321 ggcgagcgcc aggaaggtgt cctcgacggt gcgcgccatc ggcccgagct tgtcgagcga
     1381 ccagcacagg gtcatcgcgc cggtccgcgc gacgcgtccg aaggtcggac gcaggccggt
     1441 gacgccgcag cgcatcgccg gcgcgatgat gctgccgccg gtctcgctgc cgagcgcgaa
     1501 gccgacgcag cccgccgcgg tcgccgagcc cggtccggcg ctcgagcccg aggcaccctc
     1561 ctcgagcagc cacgggttgt tggtctggcc gccgtaccag atgtcgttga gggcgagcgc
     1621 gccgaggctg agcttggcga ccagcacggc gccggcctgg tgcagccgcg ccacggtcac
     1681 ggcgtcggtc ctgggcacgc ggttccggaa cggctcggcg ccccacgtcg tcgggatgcc
     1741 ggcggtgtcg accaggtcct tggcgcccca cgggatgccg tgcagcggcc cgcggtactt
     1801 gccggcggcg atctcggcgt cggcctgctt ggcctgcgcg agggcgaggt cgcgggtcag
     1861 cgtgatgacg gcgttgatct tgggctggaa ccgctggagg cgctcgaggt agatccgggt
     1921 cagccggtcc gaggtgagct ggcggctctc gatccagcgc gccagctggg tcaccggcgc
     1981 gaacgcgatg tcgtcgtcct tggccggcag cttgcccggc gggccggggc tgcgtacgaa
     2041 gcggttgcgg gcgggcccga cggtgaggcc gggcagggca gggttccagt ggctcgccgg
     2101 cgacaccgtc gggtcgagcg cgaccttgcg cgggccggtc cggcgctcgt acagcggcgc
     2161 catcgacacc cgccagttgc cggcggcctg ggcgcgttcg gccggggtgt agctcacctg
     2221 catcagcttc tcggcctcgg cgatggt
//
LOCUS       DASVOR010001095         4374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_215778,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001095 DASVOR010000000
VERSION     DASVOR010001095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215778"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            446..>4374
                     /locus_tag="VF516_21000"
     CDS             446..>4374
                     /locus_tag="VF516_21000"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8110228.1"
                     /translation="MPHDCDLFIVYAAADADFVRGYLLPALNLPSHRVQLIDGLPLGS
                     LVVAEIDRLVSRSRFTVAVLSPAYLADRWAMFGEQLASSHGVAQGRIIPLQLSDAELP
                     LRLKARVSLDFTIRERWESEAARLRELLDTAAPAEERVPCPYPGMRAFTSADGARFFG
                     RDDEIADLIDRLDRDEREIYVIGPSGSGKSSLVQAGLLHKLNSGSSRLHRSFTACVMR
                     PDEQPAKQLVKALHRKGLRGSLPPGQRVLIFVDQLEELFTIAEARERSEFVTMLRQLH
                     RAPWCYLLLALRADFYGTLMDSELWPARGSISRLDVAPLRGQALAKAIREPARQVGVY
                     VEARLCDRLITDAATEPGALPLVQETMRQLWERRSQRLLALAEYEALGGGGSALHVAI
                     ARRADASMHALSASQQAVARRILIRLVNFGEGRPDTRRQQSVTALQSAGDDPAEFSGV
                     LQQLVEDRLVTLDGNDRTEDTVADLSHEAVIATWPALHDFVCRRRADELLRRRFEVRV
                     AEWIERGRGTTGLLDAAQVSDAEQWIHSDAARELGSPAELGALVAASWSAIDHAERQV
                     HGTRRLLGMTYLEQGRSLLLDGHPMQALPYLVAARAEGIESPVLRILFAQASNSLPIA
                     CFPSAGAAVFSADGARVVTTSFEASGDRQVPAPGLATRHAAQVRDTSTGALVGELLGH
                     AGRTSDLGIAACSVEPTRVVTTWFSSTTDQFWRGQFGPRRATMPRARLWNTSAGRSTT
                     LPLADRGEFHVAAFSHDGARLVTANGDTIAEIWDTATGKPAAPPLVHRGPVHTAVFSR
                     DGDLVVTASDDGMARVWRAATGELVGPPLQHRGAVRAADFSPDAARVVTASFDGTARV
                     WEVGTGKPLTAALRHDGAITAVAFSADGRHIVTASVDKTARVWNAATGVPVTSSLEHD
                     AAVHAAAFSGDGTRVVTASADRTAQVWDAASGSRLTTSLEHPAAVAAAAFSPDGARVF
                     TIDAHHTARVWDATAAWPMHRLLERGAVYDARFSADGSRLITARANKTAQVWDASTGR
                     AVTPPLMHRGAVHAVAFSRDGKRVVTAGQDHTARVWDASTGEPVTPPLEHHGEVHVAE
                     FSASGARVVTAGLDHTARVWDVVTGKPMTPPLMHHDIVGAAAFNVDGTRVVTASWDKT
                     ARIWDAATGMPVGSPLKHQDVVWTAAFNADGTRVITASSDHTAQLWNAATGRPTTGPL
                     EHRGIVHDAAFSPDGTRVVTAAFDNTARVWDAATGRPVTRPLEHQDLVFGAAFSPDGA
                     LVVTASRDHSARVWDAATGNPVTPPLRHRGFVRAAVFSPDGTHIVTK"
BASE COUNT          643 a         1472 c         1603 g          656 t
ORIGIN      
        1 ccggggtctc gaccccgtcg tcgaccgaga tggccggcga cggcgcggtc gagggcgacg
       61 gcctcgacac cggctgggac tgagcagctc gccggctcgg cgcccagcgc gcatctcgtg
      121 caccgcggtg ctgggtgtgc agggcagtaa tgccacttca gcggtggatg tgcgcgagat
      181 ccccgccggc cgtacaacac agttccagcg cgtgcgggca ccgcgttgtc ggaaatcgga
      241 gtcgagcggc gcctgatgac agcggaggct ggcgggcggc agtcgttcgc cttcgtggag
      301 cgtgcgatca ggcgcggcaa cctgggccgc tcgtgttgca gatggggata ggatgccagc
      361 cagagtccgt gttgcgcgag gaatggtaat gatagcgaga cgaaagccgg tcgacgaaca
      421 tgcagagtct gcaatccggc gtccgatgcc ccacgattgc gacctgttca tcgtgtacgc
      481 cgcagccgac gcagatttcg tgcgcggcta tctgctgccg gcgctcaatc tgccgtcgca
      541 tcgcgtccag ctgatcgacg gtctgccgct cggctcgctg gtcgtggcgg agatcgaccg
      601 cctggtgtcg cgcagccgct tcacggtcgc ggtgctgtct ccggcgtacc tcgcggatcg
      661 ctgggcgatg ttcggcgagc agctcgcgag ctcgcacggc gtcgcacagg gccggatcat
      721 cccgctgcag ctctccgacg cggagcttcc gcttcgcctc aaggcccggg tctcgctcga
      781 cttcaccatc cgcgagcgct gggagtcgga ggccgcgcgg ctgcgcgagc tgctggatac
      841 cgccgcaccc gccgaggagc gggtgccatg cccgtatccc ggcatgcgcg cgttcacctc
      901 cgcggacggc gcccggttct tcggccgaga cgacgagatc gccgacctga tcgatcggct
      961 cgaccgcgac gagcgcgaga tctacgtgat cggtccgtcc ggatcgggca agtcgtcgct
     1021 ggtgcaagcc ggcctcctgc acaagctgaa cagcggctcc tcgcgactcc atcgatcatt
     1081 caccgcgtgc gtgatgcgcc ccgacgagca accggcaaag cagctcgtga aggcgctgca
     1141 ccgcaagggc ctgcggggct cgcttccgcc cggacagcga gtgttgatct tcgtggacca
     1201 gctcgaggag ctgttcacga tcgccgaggc ccgcgaacgc agcgagttcg tgacgatgct
     1261 tcgccagctg caccgcgcac catggtgcta tctgttgctc gccctgcggg ccgacttcta
     1321 cgggacgctg atggacagcg agctgtggcc ggcacggggc agcatctccc ggctggacgt
     1381 cgcaccgctg cgcggtcagg cgctcgcgaa agcgattcgc gaacctgccc gccaggtggg
     1441 cgtgtacgtc gaggcacggc tctgtgatcg actcatcacc gacgccgcca ccgagcccgg
     1501 tgcgctgccg ctggtccagg agacgatgcg ccagctatgg gagcggcgca gtcagcggct
     1561 gctcgcgctg gccgagtacg aggcgctggg cggcggtggg agtgcgctgc acgtggcgat
     1621 cgcgcggcgc gccgacgcct cgatgcacgc gctgtccgcc tcgcagcagg cggtggcccg
     1681 tcgcatcctg atccggctcg tgaacttcgg cgagggccgg cccgatacgc gccggcagca
     1741 gagtgtgacg gcgctacaat ccgccgggga cgaccccgcc gagttctccg gcgtgctgca
     1801 gcagctcgtc gaggaccgcc tcgtcaccct ggacggcaac gaccgtaccg aggacaccgt
     1861 cgcggatctt tcgcacgagg cggtgatcgc gacctggccg gcgttgcatg acttcgtttg
     1921 ccggaggcga gcggacgagc tgctgcggcg gcggttcgag gttcgggtcg ccgagtggat
     1981 cgagcgcggc cggggcacca cagggttgct cgacgccgcg caggtctccg acgccgagca
     2041 gtggatccac agcgatgcgg cgcgcgagct gggatctccg gccgagctcg gcgcactggt
     2101 cgcggcgagc tggagcgcca tcgaccatgc agagcgacag gttcatggca cgcggcgcct
     2161 cctcggtatg acctacctgg agcagggccg ctcgctgttg ctcgatggtc atcccatgca
     2221 ggcgttgccc tacctcgttg ccgcgagggc cgaggggatc gagagtccgg tgctccgcat
     2281 cctgttcgcg caggcgtcga atagcctgcc gatcgcctgc ttcccgtcgg ccggggccgc
     2341 cgtgttcagc gcggacggtg cgcgggtggt gaccacgagc ttcgaggcgt cgggggaccg
     2401 tcaggtgccg gcgcctgggc tggccacccg gcacgcggcg caggtccggg atacgtcgac
     2461 cggcgcgctg gttggtgagc tgctcggtca tgccggcagg acgagcgatc tcgggatcgc
     2521 cgcgtgcagc gtcgaaccga cgcgcgtggt caccacgtgg ttctcgtcga cgaccgatca
     2581 attctggcgc gggcagttcg gaccgcgccg cgcaaccatg ccgcgggcga ggctgtggaa
     2641 cacctcggcc ggcagatcga cgaccctccc gctggcggat cgcggcgaat tccacgttgc
     2701 tgcattcagc cacgacggcg cgcgcctggt caccgccaac ggcgacacca tcgcggagat
     2761 ctgggataca gcgacgggca agccggcggc gccgccgctg gtccatcgcg ggccggtcca
     2821 caccgcggtg ttcagccgcg acggtgatct ggtcgtcacc gccagcgacg acggcatggc
     2881 gcgggtctgg cgcgcggcga ccggcgagct ggtgggaccg ccgctgcagc atcgcggggc
     2941 tgttcgcgcc gcggacttca gcccggatgc tgcgcgcgtg gtgaccgcga gcttcgatgg
     3001 caccgcgcgg gtctgggagg tcgggacagg caagccgttg accgctgcgc tgcgccacga
     3061 cggcgccatc acggcggtcg cgttcagcgc ggacggacgg catatcgtca ccgccagtgt
     3121 ggacaagacc gcgcgggtct ggaacgcggc gaccggggtt cccgtgacct cgtcgctcga
     3181 acatgacgcg gcggtgcacg cagcggcgtt cagcggcgat ggaacccggg tggtcaccgc
     3241 gagcgcggac cgcaccgcac aggtgtggga cgccgcgagc gggagccgac tgaccacatc
     3301 actggagcat ccggcggcgg tcgcggcggc ggcgttcagc ccggacggcg cacgggtatt
     3361 cacgatcgac gctcaccaca cggcgcgcgt gtgggatgcg acagcggcgt ggccgatgca
     3421 ccgcctgctg gagcggggcg cggtctacga tgccaggttc agtgccgatg gctcgcgcct
     3481 gatcaccgcg cgagccaaca agacggcgca ggtgtgggac gcgtcgaccg gcagggccgt
     3541 cacgcctccg ctgatgcatc gcggcgccgt ccacgcggtt gcgttcagtc gcgatggcaa
     3601 gcgggtggtc accgcgggtc aggatcacac cgcgcgggtc tgggatgcat cgaccggaga
     3661 acctgtgacc ccaccgctcg agcatcacgg cgaggtccat gtcgcggagt tcagcgccag
     3721 cggcgcgcgc gtcgtcactg caggtctgga ccacacggca cgggtctggg atgtcgtgac
     3781 cggcaagccg atgaccccgc cgctcatgca tcacgacatc gtgggggctg cggcgttcaa
     3841 tgtcgacggc acgcgcgtgg tgaccgcgag ctgggacaag accgcgcgga tctgggacgc
     3901 agcgaccggg atgccggtcg gcagcccgct caagcaccag gacgtcgtgt ggacggcggc
     3961 gttcaacgcc gacggcacgc gcgtgatcac ggcgagctcg gaccacacgg cacagctatg
     4021 gaacgcggca accggccggc cgacgaccgg cccgctcgag catcgtggca tcgtgcatga
     4081 tgctgcgttc agccccgatg gtacgcgcgt cgtcaccgcc gccttcgaca acaccgcgcg
     4141 cgtgtgggat gcggcgacgg ggcggccggt gacccgaccg ctggaacatc aggatctggt
     4201 ctttggcgcc gcgttcagcc cggatggcgc gctggtggtg accgcgagcc gcgaccactc
     4261 ggcgcgggtc tgggacgcgg cgaccgggaa cccggtgacg ccgccgctgc ggcaccgtgg
     4321 cttcgttcgt gcggcggtgt tcagtccgga tgggacccat atcgtgacga agag
//
LOCUS       DASVOR010001096         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_186653,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001096 DASVOR010000000
VERSION     DASVOR010001096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186653"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VF516_21005"
     CDS             complement(<1..416)
                     /locus_tag="VF516_21005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110229.1"
                     /translation="MSLRAVSLLFVTLVATAVASPASAQIEPAPAVPAVADDDLPLGH
                     FQIRSFGAADGLRNLIVLGITQDGDGMLWVATDDGVYRYDGQQFTHYSMQDGLPAMGV
                     RVLGIAPNGALCAGTRDGMACWNGSRFSPQGAEGVPR"
     gene            complement(588..848)
                     /locus_tag="VF516_21010"
     CDS             complement(588..848)
                     /locus_tag="VF516_21010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110230.1"
                     /translation="MEKIELALSPRPLRLTLTLSQSVDLEGDVVVEPVVDLGREPRSF
                     ARSILENDGIWARSRSRVGSTSYVAVKAHDGVNDEVNLKVRD"
     gene            complement(850..1467)
                     /gene="tmk"
                     /locus_tag="VF516_21015"
     CDS             complement(850..1467)
                     /gene="tmk"
                     /locus_tag="VF516_21015"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEX8110231.1"
                     /translation="MTRLIVLEGLDGAGTTTQARRLVEHLQGRGQLAHLTREPSDGPI
                     GRLIREMLTGHHALPGARISQSTFGLLFAADRLDHLQREVEPRLAAGATVVSDRWYHS
                     SLAYQGTGADRDWIATLNARARRPDLTIFLQVRPEIAAQRRVAAGRVQELFEDLQMQQ
                     EVDAGYRATISELTALGERIEVIDGELPPDAVFQQIVALTESVDH"
     gene            complement(1464..>2250)
                     /locus_tag="VF516_21020"
     CDS             complement(1464..>2250)
                     /locus_tag="VF516_21020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110232.1"
                     /translation="RAAWMAYVTGPPPPPAPVAKLNLPGGKKTKKPKPLWLTYRELAD
                     NELRKAANELLHEDYPLEDPTLEDEDDDHPRRSKTVKVDVEELTQRLFAYYDAARAKQ
                     NAAEWAEAKAKFDAGDLPSATQRVDRMLAADPQRGKPAERAEMARLYLAWGKQLEAKQ
                     RWADAAVAYSKAQGLDPGGASANDALAAHHYTLGKALEAQGKDGGPDFRRAIALKPDY
                     APARSAAREVAAADRPSWMLYAAAGAAVVAMLLFAAAMMRRRA"
BASE COUNT          320 a          765 c          808 g          357 t
ORIGIN      
        1 cgcggcacgc cctcggcgcc ctggggcgag aaccgcgagc cgttccagca cgccatgccg
       61 tcgcgcgtcc cggcgcacag cgcgccgttg ggggcgatgc cgagcacgcg cacgcccatc
      121 gccggcaggc cgtcctgcat cgagtagtgg gtgaactgct ggccgtcgta gcggtacacg
      181 ccgtcgtcgg tggcgaccca cagcatgccg tcgccgtcct gggtgatgcc gagcacgatc
      241 aggttgcgca ggccgtcggc ggcgccgaac gagcggatct ggaagtggcc gagcgggagg
      301 tcgtcgtcgg ccacggcggg caccgccggt gccggctcga tctgcgcaga cgccggggac
      361 gcgaccgcgg tggcgacgag cgtgacgaac agcaggctca cagctcgcag cgacatcact
      421 tcaaaattcg accggtgcgg tgatcgtgta aagcttgttt tttcgcggtg tgtgacggag
      481 ctcgtgaatg caatttccac acgcagacac cgcggccgac actgccgcac gacacggcag
      541 gacaagctct cgaatcgagt caagatgtgt cgctcaaagc gttcgatcta gtccctcacc
      601 ttgaggttga cctcgtcgtt gactccatcg tgggccttga cggcgacgta ggaggtcgac
      661 cccaccctcg acctcgacct tgcccatatc ccgtcatttt cgaggatgct cctcgcaaat
      721 gaccggggtt cacgtccaag gtcgacgacg ggctcaacga ctacgtcgcc ctcaaggtca
      781 acgctctgcg acaacgtcaa ggtcaacctc aacggcctcg gactcagcgc gagctcaatt
      841 ttctccacat cagtgatcga ccgattcggt caacgcgacg atctgctgga acaccgcgtc
      901 gggcgggagc tcaccatcga tcacctcgat ccgctccccg agcgcggtga gctcggagat
      961 cgtcgcgcgg tagccggcgt cgacctcctg ctgcatctgg aggtcctcga acagctcctg
     1021 cacgcggccg gcggcgaccc ggcgctgcgc ggcgatctcg ggacggacct ggaggaagat
     1081 cgtgagatcc ggccgacgcg cccgcgcgtt gagcgtggcg atccagtcgc ggtcggcgcc
     1141 ggtgccctgg taagcgagcg acgagtggta ccagcgatcg gacaccacgg tggcgccggc
     1201 ggcgagccgg ggctcgacct cacgctgcag gtggtcgagg cggtcggcgg cgaacagcag
     1261 gccgaacgtc gactggctga tccgggcgcc ggggagcgca tggtgcccgg tcagcatctc
     1321 gcggatcagc cggccgatcg gcccgtcgct cggctcgcgc gtcaggtgcg cgagctggcc
     1381 acggccctgc aggtgctcga ccagccgccg ggcctgggtg gtggtgccgg ccccgtcgag
     1441 gccttcgagg acgatcaacc gggtcatgcg cgccgccgca tcatggctgc ggcgaataac
     1501 aacatcgcca cgaccgcagc gccagcggcg gcgtacagca tccacgacgg ccggtcggcg
     1561 gcggcgacct cgcgggccgc gctgcgcgcc ggggcgtagt cgggcttgag cgcgatcgcg
     1621 cgccggaagt cggggccgcc gtccttgccc tgggcctcga gcgccttgcc cagcgtgtaa
     1681 tggtgggcgg cgagcgcgtc gttggcggac gcgccgccgg gatcgagccc ctgggccttg
     1741 gagtaggcga ccgcggcgtc ggcccagcgc tgcttggcct cgagctgctt gccccaggcg
     1801 aggtacagcc gggccatctc ggcgcgctcg gcgggcttgc cgcgctgcgg gtcggcggcg
     1861 agcatgcggt cgacccgctg ggtcgcggat ggcagatcgc cggcgtcgaa cttggccttg
     1921 gcctccgccc actccgccgc gttttgcttg gcgcgcgcgg cgtcgtagta cgcgaacagc
     1981 cgctgggtca gctcctcgac gtcgaccttg acggtcttgg accgccgcgg gtggtcgtcg
     2041 tcctcgtcct cgagcgtcgg gtcctccagc gggtagtcct cgtgcaagag ctcgttggcg
     2101 gccttgcgga gctcgttgtc ggcgagctcg cggtaggtca gccacagcgg cttgggcttc
     2161 ttggtcttct tgccgccggg caggttgagc ttggcgaccg gcgcgggcgg cggcggcggg
     2221 ccggtgacgt acgccatcca ggcggcgcgc
//
LOCUS       DASVOR010001097         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_127786,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001097 DASVOR010000000
VERSION     DASVOR010001097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127786"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..>1624
                     /locus_tag="VF516_21025"
     CDS             88..>1624
                     /locus_tag="VF516_21025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110233.1"
                     /translation="MTSFRTVLIGLLACSACTAFDHELPECTTNTQCTAKLSAGGSTV
                     QGVCVQPEAKCVALASEDCKTLTGNYMESPVTVPDDAVVIASLLSTTGAQGATNLARQ
                     QSAMMAVEEINKSGGIMQSKTPGDARKLVMLSCDENANLLRVTTHLINELHIAGIVGP
                     NLSQDTIDVTAGNNGLPSSAKAGTAMLSPSAVAAQIATIPSNGLSFMMVPSDSQRVPL
                     MKLRINALESQLKTQRQHPIKLAIYYRNDALGQGTHDGLTNSSLTIDNLSLSDARNQN
                     LFRDDVYDPKATATDNSNLVQQYITFQPDIIVVIGTAEAVTAFTNPLEAKWQTQIPTV
                     PKPYYVAIDSTKVPELLTAVGNATDDQRRRWSGTGIAPTPDSALAFNAFKTAFGERWT
                     DSAGKPLPATVSGMGPAYDAVYTIALSLVGKLPGKQDVAVGATIAAGMPRLASNPQAC
                     SSDANGIVPPCYLLTDHTRTLTDSMTKLLDGKPVTEIGTFGALTWDAGAKSSGLIELW
                     CIDG"
BASE COUNT          321 a          570 c          513 g          220 t
ORIGIN      
        1 aggccgcgcc gcccaagccg gcgcccgccc aacccgacag cccgcccctg gagaccaatc
       61 cctatgtcta caagtgaggt tggtggcatg acgtccttcc gcaccgtcct gatcggactg
      121 ctcgcgtgca gtgcctgcac ggcgttcgac cacgagctcc cggagtgcac caccaacacg
      181 cagtgcacgg ccaagctgtc agcaggcggc tcgaccgtgc agggggtatg cgtccagccc
      241 gaggcaaaat gcgtggcgct cgccagcgaa gactgcaaga cgctcacggg caactacatg
      301 gaatcgccgg tgactgtgcc cgacgacgca gtggtgatcg cgtcgctgct ttcgaccacc
      361 ggcgcccagg gggcgaccaa tctcgcgcgc cagcagtccg cgatgatggc ggtcgaggag
      421 atcaacaagt cgggcgggat catgcagagc aagacaccgg gcgatgcacg caagctcgtc
      481 atgctgtcgt gcgatgagaa cgccaacctg ctccgggtca cgacgcacct gatcaacgag
      541 ctgcacatcg cggggatcgt cggtccgaac ctcagccagg acacgatcga tgtcacggcg
      601 ggcaacaacg ggctgcccag cagcgccaag gcaggcaccg cgatgctgtc accgtcggcg
      661 gtggccgcgc agatcgccac gatcccgagc aacgggctca gcttcatgat ggtgcccagc
      721 gatagccagc gcgtgccgct gatgaagctc cggatcaacg ccctggaaag ccagctcaag
      781 acgcagcgcc agcacccgat caagctcgcg atctactacc ggaacgacgc gctcggccag
      841 ggcacgcacg acggcctgac caattcgtcg ctgacgatcg acaacctctc gctgtcggac
      901 gcgcgcaacc agaatctgtt ccgcgatgac gtgtacgacc cgaaggcgac cgcgaccgac
      961 aacagcaacc tcgtccagca gtacatcacc ttccagcccg atatcatcgt cgtgatcggc
     1021 accgccgagg cagtcacagc cttcacgaac ccgctggagg cgaagtggca gacgcagatc
     1081 ccgacggtac ccaagccgta ctacgtcgcg atcgactcga ccaaggtccc cgagctgctc
     1141 accgcggtgg ggaacgccac ggacgaccag cgcaggcggt ggagcggtac cggcatcgct
     1201 ccgacgcccg acagcgcgct ggcgttcaac gcgttcaaga ccgcgttcgg cgagcgctgg
     1261 acggactcgg cggggaagcc gttgcccgcg acggtgtccg gcatgggccc cgcctacgac
     1321 gcggtctaca cgatcgccct gtcgctggtg ggcaagctgc ccggcaagca agatgtggcc
     1381 gtcggcgcga cgatcgcggc cggcatgccg cgcctggcga gcaaccccca ggcgtgttcg
     1441 tccgacgcga acgggatcgt accgccgtgc tacctgctca ccgaccatac ccgcacgctg
     1501 accgacagca tgacgaagtt gctcgacggc aagcccgtca ccgagatcgg caccttcggc
     1561 gccctgacgt gggacgccgg cgccaagtcg agcggcctga tcgagctgtg gtgcatcgac
     1621 ggca
//
LOCUS       DASVOR010001098         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390685,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001098 DASVOR010000000
VERSION     DASVOR010001098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390685"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VF516_21030"
     CDS             complement(<1..603)
                     /locus_tag="VF516_21030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110234.1"
                     /translation="MRAWVLALALGACGFSSSAGIGKGSNGSVPDAGDPTPPDDSGGG
                     GSPDAGPPVHVCLGTFVQVCADAPRGALNLMTGTIDTSDTSASSKCLPATAYTTTPVV
                     DACVIASQSITIPSGNTVSVIGTRRLILLTDEALTIAGTLDVASHRGGNAGPAADSGP
                     CPSPGSNPTFKTEGGGGWGGTFGSSGNNGGNTPGGGIGGGA"
     gene            complement(734..1966)
                     /locus_tag="VF516_21035"
     CDS             complement(734..1966)
                     /locus_tag="VF516_21035"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8110235.1"
                     /translation="MSQALRDLLRAETDRLKDAGLYKREVVFSRSGGMAAGGMVDGKT
                     GQPVVNYTTHDFLGLSVDPQLQQAAIDAIREYGVGLSSQRVMCGTLQIHKDLEHWLRD
                     FLKVPDVILYGSGYHANLGVFAPLFGAKDCIICDAGVHPSLAEGARLAGARLVTFRSD
                     DPDDLEDILRRSKWARFRAVVTNGVHPFTGRISDLASICELARRHDAMVVVDDALGIG
                     VLGERGRGSTEHAGVLDRVDLITGTFSKALGGASGGFAAGKSEIIEWLRQKSSPYMFS
                     AALPPAMAKVALAALQILESGEAPLAGLRDNVRTLFDGLIERKFRVLGGQHPLLIVEI
                     GPYEILRETVNHLYDRGIYCHGLCYPVVPEGEARIRLMVSALHSEAHIQQTLDAFASA
                     REVAAFAVDALDALGGLQ"
BASE COUNT          297 a          737 c          737 g          290 t
ORIGIN      
        1 ggcgccgccg ccgatgccgc cgccgggcgt gttgccgccg ttgttgccgg aggaaccgaa
       61 cgtgccgccc cagccgccgc cgccctcggt cttgaacgtg ggattgctcc cgggcgacgg
      121 gcagggaccg ctgtccgccg cggggccggc gttgccgccg cgatgactgg ctacatcgag
      181 cgttccggcg atcgtcagcg cttcatcggt gagcaggatc agtcgcctgg tcccgatcac
      241 cgataccgtg ttcccgctcg ggatcgtgat cgactgactg gcgatcacgc aggcgtcgac
      301 cacgggggtg gtggtgtagg cggtcgcggg caggcacttg ctcgacgcgg aggtgtccga
      361 ggtgtcgatc gtcccggtca tcaggttgag cgcgccgcgc ggcgcatcgg cgcatacctg
      421 gacgaaggtg cccaggcaga cgtgcaccgg cggaccggcg tcgggactgc cgcctccgcc
      481 ggagtcgtcc ggtggggtcg ggtcccctgc gtccggaacg ctgccgttgc tccccttgcc
      541 gatcccggcg gaggaggaaa acccgcaggc cccgagcgcc agcgccagaa cccatgcgcg
      601 catgtaagta ttttaccgca actgggacgc gccgcttccc gcgatcgggg ccgcgacgtg
      661 gcttgggtca cgcgccgcgg gtactgcacc cggcgcgggt ccggagcggc gtatccgcgt
      721 gcagcgcccg cggctactgc agcccgccga gcgcatcgag cgcatcgacc gcgaacgcgg
      781 cgacctcgcg ggccgacgcg aaggcgtcga gcgtctgctg gatgtgggcc tccgagtgca
      841 gcgccgacac catcaggcgg atccgcgcct cgccttcggg gaccaccggg tagcacaggc
      901 cgtggcagta gatcccgcgg tcgtagaggt gattgacggt ctcgcgcagg atctcgtacg
      961 gcccgatctc gacgatcagc agcggatgct ggccgcccag cacccggaac ttgcgctcga
     1021 tcaggccgtc gaacagcgtg cggacgttgt cgcgcaggcc cgccagcggc gcctcgccgc
     1081 tctccaggat ctgcagcgcg gccagggcga ccttggccat cgccggtggc agcgcggccg
     1141 agaacatgta gggcgacgac ttctggcgca gccactcgat gatctcggac ttgccggccg
     1201 cgaacccgcc ggacgcgccg cccagcgcct tgctgaacgt gccggtgatc aggtcgacgc
     1261 ggtcgagcac cccggcgtgc tcggtcgagc cgcggccgcg ctcgcccagc acgccgatgc
     1321 ccagcgcgtc gtcgaccacg accatcgcgt cgtggcgcct ggcgagctcg cagatcgatg
     1381 cgaggtccga gatccggccg gtgaacggat ggacgccgtt ggtcaccacc gcgcggaacc
     1441 gcgcccactt cgagcggcgc aggatgtcct cgaggtcgtc ggggtcgtcg ctgcggaacg
     1501 tcaccagccg cgcgccggcg agccgggcgc cctcggccag gctcgggtgc acgccggcgt
     1561 cgcagatgat gcagtccttg gcgccgaaca gcggcgcgaa cacgccgagg ttggcgtggt
     1621 agcccgagcc gtagaggatg acgtcgggca ccttgaggaa gtcgcgcagc cagtgctcca
     1681 gatccttgtg gatctgcagc gtgccgcaca tcacccgctg cgacgacagg ccgaccccgt
     1741 actcccggat cgcgtcgatc gcggcctgct ggagctgcgg atcgaccgat agcccgagga
     1801 agtcatgggt cgtgtagttg accaccggct ggccggtctt gccgtcgacc atgccgcccg
     1861 cggccatgcc gccggaccgc gagaacacga cctcgcgctt gtacagcccg gcgtccttga
     1921 gccgatcggt ctcggcgcgc agcagatcgc gcagcgcctg gctcacgggc cacctccgac
     1981 gaaccagacc agcgccgcga cgcagcgcga ccacggcagg ctcggcacgc acgccgcgtc
     2041 gccggcctgg cacgcgtgca g
//
LOCUS       DASVOR010001099         7609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_215793,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001099 DASVOR010000000
VERSION     DASVOR010001099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7609
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215793"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..805
                     /locus_tag="VF516_21040"
     CDS             62..805
                     /locus_tag="VF516_21040"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110236.1"
                     /translation="MMQDGDEKRRAPREPVTLFVEYEGADDLVGDFTDNLSSGGTFVT
                     TNRSLPVGTQVQLVLSFPGLLEPIAIEGTVRWNRGESAVGDPGAGIEFVPGPARNTLA
                     ALVDRIRARDPRVMAKLLRVLVVEDNRHVAELIQQGLTGSTRRDFGGGVTFAFRNAED
                     GRSAIALLRDEPFDAVIIDVYLPVVDGPTVIAVARKELGLIDLPIIAVSGGGESARDA
                     ALDAGANIFLDKPMRLRRVIETMQRLIPP"
     gene            955..3108
                     /locus_tag="VF516_21045"
     CDS             955..3108
                     /locus_tag="VF516_21045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX8110237.1"
                     /translation="MKTRLLAMILATMAGCGSNKPAATAPPAPPPSSAPEVASSAPAP
                     SSDPAPPPSNRPKPEIGSWGFDVGGMDTSVAPGASFYRYANGKWLTTTQIPADKPDYG
                     MFTMLADRSEERTRQIIESASGPSGGDGQRIADYYKAFMDEAAIEARGLKPVQPEIDR
                     IAKIKDATGVATEFAASARRLGGRGALNATPFQTVVGQDAREPDKYIGHIGQGGLGLP
                     DRDMYDVKHEQFAKVRDGYQKYIAKIFTLIGAKDADKRAAAVYGLEEKIAQTHWTRVQ
                     NRDANKRYNKMTIDELVKLAPGMAWKPWLHAVGLDGQTQVIVGQPSAVAGEAQLVKSE
                     PLAVWKDYLTFHAVSAAAPFLPKKFVEAHFEMYEKTLRGTPQIKERWKRAVDLITGGM
                     GEAVGQIYVAKYFTPETKAHADALVKNLLSAMSQRLDGLAWMGTETKTKAKAKLATYN
                     PKIGYPVVWRDYSALTIKPDDAVGNAKRTGEFEYNRRLARLGKPIDRNEWNMTPMTVN
                     AYYNAELNEIVFPAAILQPPFFDPSADDAVNYGGIGAVIGHEISHGFDDQGSKFDATG
                     KLENWWTKDDADKFKTATAKLVAQYNAYCPFPAANGKPAQCVKGELTLGENIGDLAGL
                     TIAYNAYKLSLGGKTPPVLDGFTGDQRFFLGWAQVWRRLVRDEEAANRLTTDPHSPSE
                     FRTSVVRNLDAWYDAFKPQPGDALFLAPDQRVRIW"
     gene            complement(3121..>7609)
                     /locus_tag="VF516_21050"
     CDS             complement(3121..>7609)
                     /locus_tag="VF516_21050"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8110238.1"
                     /translation="AEQRTLGRPAVVKVVHRAMATRLDISERFVREARLASRFDHPYA
                     AHVYAFGVEHDGLMWIAMEFVDGTPLGQLIQQSGPLAVDRFVPLFERLCEVVQSAHDQ
                     GIVHRDIKPSNVMVIERAGRLMPKLLDFGIARLVLDAAPANAPAETWKPAPVGSPSER
                     SIAGSWDLTQVGQVLGSPQYMAPEQWRDATSAGPKADQYALALLAYEVMTGAHAFAGR
                     TIEALAHQHLHAPFPSLPPRLPAALRDVLARAGDKRPEGRFANLNELAAAVRIVALGK
                     ATELEEPIEALPEAVAPYPGLAAYTATDHASFVGRERDVEELLNRLDAHSIVTVVGPS
                     GSGKTSFLAAGLIPALEPRRHSELIRPGSDPLGVLAAIAQRGHAPPYRDGLLHDTMTS
                     PVAIATALVGLAETRGVVMVLIVDQAEELFTMCADDTRRTAFAEVLVRASASPRIRLV
                     LALRDDFLCRIEQLPAWRGLLGRAVHVLGIPRREDLERMLTVPARRRGFRFDDPDLPR
                     EIVDQVADRPGALPLIAFTAAQLWERRDRRLRRLTRSAYQQIGGVTGALVQHADGVMD
                     RMSVPDRRQARLVFRRLFTAEGTRALINRTELESSLGGPSPATVIDRLLAARLIVSHD
                     DDHGDRIEIIHETLATTWPRLAAWRREDADGLRLQDQLTVAARHWNERARPADLLWGG
                     GALSDLRRWRDSGDHSMTPVEHAFVRACIASASQRKRARIALVTSAFALLVAGVVALQ
                     SENREIADQRAVAVDRLRANFEERGRAAIGDGDHPRGLLYLAEASRLGAHGPALDLLT
                     SHAMVSLDAGVGVIGHGHAGIVAMDIGATAIVTLGSDLTLSRWDHAGHAVALADRTSC
                     ATLVGDLAVSVSTQGDVAAIDPRGHIRWRAERPWAAPGTRCGIAGSEAARLVAAFGKT
                     VTLWDLDSGRPRGELRHDQDVSAIAIAADGSQLATGDLSGVTRIWDTATAALVTTCEP
                     HTGVVRTIKFAADQRSVVTGGNDADVRICDTATGATLRRLIGHSHPVLTVDETTDGRT
                     IVSGARDGKPRIWDARTGLLVRVLEGHRGAIMSVKFSPDNERVLTLGLDGTARVWDRE
                     GMALGSLQGHGGPIYAGAWDLDGHHVITSSIDGAIRRWDPDRGRTTVSRHAHEGAIVD
                     LAVSGDDRWVLTAGADRRAVLWDRRSLQPIAELPHGAKVSLVRFAPDGRTALTADDDG
                     NARLWQVPAGTLIARLPAGVTAASYLPGGAVVTAGDGMVRFWTASGRELGAVPSGPDV
                     DRLVVDPTGRWLFASGATSSVLVIDAVRRAPLVRLEVGDKEVLGLAANDSCVAITDGA
                     AIRLWRLGTWAPVTTLVGHKNPVSNVWFLSDGRLVSAAADATLVWGSDGSLRARLADT
                     NYVFALATAPDGTLFATTGSDGAIRVWDAVTYRLLLQLPGHGLPALVLQLTRDGAATI
                     SGGNDGRIVTWQLTRRPRSTFELARIVRCRVPLRLEGDVALPRDLDFEDPACRALAPD
                     R"
BASE COUNT         1228 a         2740 c         2625 g         1016 t
ORIGIN      
        1 gcgggctaat ggcggttcgc ttgcgatccg gcaagcgcca agggatcctt ggcaatcgca
       61 gatgatgcag gacggcgacg aaaaacggcg cgctccccgg gagccggtga cgttgttcgt
      121 cgagtacgag ggcgccgatg acctggtcgg cgacttcacc gacaacctgt cgtcgggcgg
      181 caccttcgtg accaccaacc gctcgctgcc ggtcggcacc caggtccagc tcgtgctgag
      241 cttccccggc ctgctcgagc cgatcgcgat cgagggcacc gtgcggtgga accgcggcga
      301 gagcgcggtc ggcgatcccg gcgccgggat cgagttcgtc cccggtccgg cgcgcaacac
      361 gctcgccgcg ctggtcgatc ggatccgcgc ccgcgacccg cgcgtgatgg ccaagctgct
      421 ccgcgtgctc gtggtcgagg acaaccgcca tgtcgccgag ctgatccagc agggcctgac
      481 cggctcgacc cggcgcgact tcggcggcgg cgtgacgttc gcgttccgca acgccgagga
      541 cggccggtcc gcgatcgcgc tattgcgcga cgagccgttc gacgccgtga tcatcgacgt
      601 ctacctgccg gtggtcgacg gcccgacggt gatcgcggtc gcccgcaagg agctcggcct
      661 gatcgacctg ccgatcatcg cggtgtcggg gggcggcgag tcggcgcgcg acgcggcgct
      721 cgacgccggc gccaacatct tcctcgacaa gccgatgcgg ctgcgccggg tgatcgagac
      781 catgcagcgg ctgatcccgc cctgagatcg gcgtccgcgg cggcgcgcac agcatccggc
      841 cgtccggtcc gacgatggcg cgtcgcggca gcgctgcttc cggcggcggc aggtctaggc
      901 aaaaccgccg aggcctggta cgccagcggc accttacagc ccccggaggc ccccgtgaag
      961 acccgattgc tcgcgatgat cctcgcgacg atggccggct gcggatcgaa caagccagcg
     1021 gccactgccc cgcccgcccc ccccccatca tccgcacccg aggtcgcctc gtcggcgccc
     1081 gcgccgtcct cggatccagc cccgccaccg tcgaaccgtc cgaagcccga gatcggcagc
     1141 tggggcttcg acgtcggcgg catggacacc tcggtcgcgc cgggcgcgag cttctaccgc
     1201 tatgccaacg gcaagtggct gaccacgacc cagatccccg ccgacaagcc cgactacggc
     1261 atgttcacca tgctcgccga ccgctccgag gagcgcaccc ggcagatcat cgagagcgcg
     1321 tcggggccgt ccggcggcga cggccagcgc atcgccgact actacaaggc gttcatggac
     1381 gaggcggcga tcgaggcccg gggcctcaag ccggtccagc ccgagatcga ccggatcgcg
     1441 aagatcaagg acgccaccgg cgtggccacg gagttcgcgg cctcggcgcg gcggctcggt
     1501 ggccgcggcg cgctcaacgc gaccccgttc cagaccgtgg tcggccagga cgcgcgcgag
     1561 cccgacaagt acatcgggca catcgggcag ggcggcctcg gcctgcccga ccgcgacatg
     1621 tacgacgtca agcacgagca gttcgccaag gtccgcgacg gctaccagaa gtacatcgcg
     1681 aagatcttca cgctgatcgg tgcaaaagac gctgacaagc gcgccgccgc ggtctacgga
     1741 ctggaggaga agatcgcgca gacccactgg acgcgggtcc agaaccgcga cgccaacaag
     1801 cggtacaaca agatgacgat cgacgagctc gtcaagctcg cgcccggcat ggcgtggaag
     1861 ccgtggctcc acgcggtcgg gctcgacggc cagacccagg tcatcgtcgg ccagccgtcg
     1921 gcggtcgcgg gcgaggccca gctggtcaag agcgagccgc tcgcggtgtg gaaggactac
     1981 ctgacgttcc acgcggtcag cgcggcggcg ccgttcctgc ccaaaaagtt cgtcgaggcg
     2041 cacttcgaga tgtacgagaa gaccctgcgg ggcacgccgc agatcaagga gcgctggaag
     2101 cgcgcggtcg atctgatcac cggcggcatg ggcgaggcgg tcggccagat ctacgtcgcc
     2161 aagtacttca cgcccgagac caaggcccac gccgatgcgc tggtcaagaa cctgctctcg
     2221 gcgatgagcc agcggctcga cggcctggcc tggatgggga ccgagaccaa gaccaaggcc
     2281 aaggccaagc tcgccacgta caaccccaag atcggctacc cggtggtgtg gcgcgactac
     2341 tccgcgctga cgatcaagcc ggacgacgcg gtcggcaacg ccaagcgcac gggggagttc
     2401 gagtacaacc gccggctcgc ccggctcggc aagccgatcg accgcaacga gtggaacatg
     2461 acgccgatga cggtcaacgc gtactacaac gccgagctca acgagatcgt gttccccgcc
     2521 gcgatcctgc agccgccgtt cttcgatccc agcgccgatg acgcggtcaa ctacggcggg
     2581 atcggcgcgg tgatcggcca cgagatcagc cacggcttcg acgaccaggg ctcgaagttc
     2641 gacgccaccg gcaagctcga gaactggtgg accaaggacg acgccgacaa gttcaagacc
     2701 gcgaccgcca agctggtcgc ccagtacaac gcctactgcc cgttcccggc cgccaacggc
     2761 aagcccgccc agtgcgtcaa gggcgagctc acgctcggcg agaacatcgg cgatctcgcc
     2821 ggcctgacca tcgcgtacaa cgcctacaag ctgtcgctcg gtggcaagac cccgccggtg
     2881 ctcgacggct tcaccggcga ccagcggttc ttcctcggct gggcgcaggt ctggcgccgg
     2941 ctggtccgcg acgaggaggc ggccaaccgg ctgaccaccg atccacacag cccgtccgag
     3001 ttccggacgt ccgtcgtgcg caacctcgac gcctggtacg acgcgttcaa gccccagccg
     3061 ggcgacgcac tgttcctggc gcccgaccag cgcgtccgga tctggtagcg ccgccggccg
     3121 tcagcggtca ggcgcgagcg cgcggcacgc tggatcctcg aagtcgagat cgcggggcag
     3181 cgcgacgtcg ccctccagcc ggagcggcac ccgacaccgg acgatgcgtg ccagctcgaa
     3241 ggtcgagcgc ggcctgcgcg tgagctgcca ggtcacgatc cgaccatcgt tcccaccgct
     3301 gatcgtcgcg gcgccgtcgc gggtgagctg gagcaccagc gccggcaagc cgtggcccgg
     3361 gagctgaagc aggaggcgat acgtgacggc atcccagacc cggatcgcgc catcgcttcc
     3421 ggtggtggcg aacagcgtcc catccggggc ggtcgccagt gcaaacacgt aattggtgtc
     3481 ggcgagcctc gcccgcaggc tgccgtcgct cccccacacc agcgtggcat cggcggccgc
     3541 gctgaccagc cggccatccg acagaaacca cacgttcgac accggattct tgtgaccgac
     3601 cagcgtcgtc accggcgccc acgtgccgag ccgccacaac cggatcgcgg cgccgtccgt
     3661 gatcgcgacg cacgagtcgt tcgccgcgag gccgaggacc tccttgtcac cgacctcgag
     3721 gcgaacgagc ggcgcgcgcc gcacggcgtc gatcacgagg acggagctcg tcgctccgct
     3781 ggcgaacaac catcgcccgg tcggatcgac gaccaggcga tcgacgtccg gtccggacgg
     3841 cacggcgccg agctcgcgtc cgctcgcggt ccagaaccgg accatgccgt cgccggcggt
     3901 gacgaccgct ccgccgggca ggtagcttgc cgcggtcacc ccggcgggga gcctggcgat
     3961 caacgtcccg gcggggacct gccacagcct ggcgttgccg tcgtcatcgg ccgtgagcgc
     4021 ggtgcggcca tcgggcgcga accggaccag gctgaccttg gcaccgtggg ggagctccgc
     4081 gatcggttgc agcgaccggc ggtcccacag gaccgcgcga cggtccgcgc ccgcggtgag
     4141 cacccatcga tcgtcgccgg acacggccag atcgacgatc gcaccctcgt gcgcatggcg
     4201 cgacaccgtc gtgcgaccgc ggtccggatc ccaccgccgg atcgcaccat cgatgctgct
     4261 cgtgatcacg tgatggccgt cgaggtccca tgcgcccgca tagatcggcc cgccatgacc
     4321 ctgcagcgag ccgagcgcca tgccttcgcg atcccagacc ctggccgtgc catcgagccc
     4381 gagcgtcagg acccgctcgt tgtcgggcga gaacttcacg ctcatgatcg cgccgcggtg
     4441 gccctcgagc acccggacca gcaggccggt ccgggcgtcc cagatccgag gcttgccatc
     4501 gcgcgcgccc gacacgatcg tgcggccatc ggtcgtctca tcgacggtga gcaccggatg
     4561 gctatgaccg atcaggcggc gcaaggtcgc gccggtggcg gtgtcgcaga tccggacgtc
     4621 ggcgtcgttc ccgccggtca cgacgctgcg ctggtcggcc gcgaacttga tggtccggac
     4681 gaccccggtg tggggctcac acgtcgtgac gagcgctgcg gtcgcggtgt cccagatccg
     4741 ggtcacgccc gagaggtctc cggtggcgag ctgcgagccg tccgcagcga tcgcgattgc
     4801 gctcacgtcc tggtcgtgac ggagctcgcc gcgcggtcgg ccactgtcga ggtcccacag
     4861 cgtcacggtc ttgccaaacg cggcgacgag gcgcgcggcc tccgaccccg cgatcccgca
     4921 ccgcgtgccc ggcgcggccc atggacgctc ggcccgccac cggatgtgcc cgcgtgggtc
     4981 gatcgccgcc acgtcgcctt gtgtggacac cgagaccgcc agatcgccga ccagcgttgc
     5041 gcaggacgtc cggtcggcca gggcgaccgc atgacccgcg tgatcccacc gcgacagcgt
     5101 gagatccgag ccgagcgtga cgatcgcggt ggccccgatg tccatcgcca cgatccccgc
     5161 gtgaccatgc ccgatgaccc cgaccccggc atcgagcgat accatggcgt gcgaggtcag
     5221 cagatcgagt gcgggtccat gggccccgag cctgcttgcc tcggcaaggt agagcagtcc
     5281 acgggggtga tcgccatcgc cgatcgccgc gcggccacgc tcctcgaagt tcgcgcgcaa
     5341 ccggtcgacg gccactgcgc gctggtctgc gatctcgcga ttctccgact gcagcgcaac
     5401 gacgcccgcg accagcagcg cgaacgcgga cgtcacgagt gcgatgcgcg cgcgcttgcg
     5461 ctggctcgcc gacgcgatac atgcgcgcac gaacgcatgc tcgacgggcg tcatgctgtg
     5521 atcgccgctg tcccgccagc gccgcagatc cgacagcgcg ccgcctcccc acagcagatc
     5581 ggcaggacgc gctcgctcgt tccagtggcg cgcggcgacg gtgagctggt cctggagccg
     5641 gagaccgtcg gcatcctcgc ggcgccatgc cgccagccgt ggccacgtcg tggcgagcgt
     5701 ctcgtggatg atctcgatcc ggtcgccatg gtcgtcgtca tgggagacga tcagacgggc
     5761 ggcgagcagg cgatcgatca cggtcgccgg cgagggcccg ccgagcgagc tctcgagctc
     5821 ggtccggttg atcagcgcgc gggtgccctc cgcggtgaac aggcggcgga acaccaggcg
     5881 tgcctggcgg cgatccggga ccgacatgcg atccatgacg ccgtcggcgt gctggaccag
     5941 ggcgccggtg accccgccga tctgctggta cgcgctgcgg gtgaggcggc gaagccggcg
     6001 atcgcggcgc tcccacagct gtgccgccgt gaacgcgatg agcggcaatg cccccggacg
     6061 atccgcgacc tggtccacga tctcgcgcgg gagatcggga tcatcgaacc ggaagccgcg
     6121 gcggcgcgcg gggaccgtga gcatgcgctc gaggtcttcg cggcgtggga ttccgagcac
     6181 gtggaccgcg cggccgagca acccgcgcca cgccggaagc tgttcgatgc gacacaggaa
     6241 atcgtcccgc agcgcgagca cgagccggat gcgtggactc gcggacgctc gcaccaggac
     6301 ctccgcgaac gccgtgcggc gggtgtcgtc cgcgcacatg gtgaacagct cctcggcttg
     6361 atcgacgatc agcaccatca cgacgccacg ggtctcggcg agacccacca gggccgtggc
     6421 gatcgcgacc ggcgaggtca tcgtgtcgtg aagcagcccg tctcggtacg gcggggcatg
     6481 gccgcgctgc gcgatcgccg cgagcacgcc gagcgggtcg ctgcccgggc ggatcagctc
     6541 gctgtgccgg cgcggttcga gcgcagggat caggccggcg gccaggaacg aggtcttgcc
     6601 gctgccggac ggaccgacga ccgtgacgat cgagtgtgcg tccagccggt tgagcagctc
     6661 ctcgacatcg cgctctcgtc cgacgaacga cgcgtgatcg gtcgccgtgt acgcggccag
     6721 gcccgggtac ggtgcgaccg cctcggggag cgcctcgatc ggctcctcga gctcggtcgc
     6781 cttgccgagc gccacgatcc gcaccgcggc cgcgagctcg ttcaggttcg cgaaccgacc
     6841 ctcgggccgc ttgtcgccgg cacgcgcgag cacatcgcga agcgccgccg gcaagcgagg
     6901 cggcagcgac gggaacggtg cgtggaggtg ctggtgagcg agcgcctcga tcgtgcgtcc
     6961 ggcgaacgcg tgtgccccgg tcatcacctc gtaggcgagc agcgccagcg cgtactgatc
     7021 ggccttcggc cccgcggagg ttgcgtcgcg ccactgctcg ggggccatgt actgcggcga
     7081 tccgaggacc tgacccacct gcgtgaggtc ccacgatccg gcgatggacc gctcggacgg
     7141 cgagcccacc ggagccggct tccaggtctc ggccggcgcg ttcgcggggg cagcgtccag
     7201 gaccagcctc gcgatcccga agtcgagcag cttgggcatg agccgtccgg cgcgctcgat
     7261 caccatgacg ttggacggct tgatgtcgcg atgcacgatc ccctgatcgt gcgccgactg
     7321 gaccacctcg cacagccgct cgaacagcgg aacgaagcga tcgacggcga gtggcccgga
     7381 ctgctggatc agctgaccga gcggcgtgcc atcgacgaac tccatcgcga tccacatcag
     7441 tccgtcatgc tcgacgccga acgcatacac gtgcgccgcg tacggatgat cgaagcgcga
     7501 tgcgagccgc gcttcgcgga cgaagcgctc cgaaatatcc agccgcgtcg ccatggcgcg
     7561 atgaacgacc ttgacgaccg cgggacggcc gagcgtgcgt tgctcggcc
//
LOCUS       DASVOR010001100         2257 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_127802,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001100 DASVOR010000000
VERSION     DASVOR010001100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2257
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127802"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF516_21055"
     CDS             complement(<1..260)
                     /locus_tag="VF516_21055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110239.1"
                     /translation="MRSRRPPVSPEDQALFLAAVDGARPLDTRDRLPIPPPPPSPVRV
                     AELPPEVVLKIEGDAQRYSARAPGVSRAQLSELRAGKLRAEDT"
     gene            520..2160
                     /gene="glgP"
                     /locus_tag="VF516_21060"
     CDS             520..2160
                     /gene="glgP"
                     /locus_tag="VF516_21060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX8110240.1"
                     /translation="MVPCPRVAYFCMEYGLDPAFAIYAGGLGVLAGDHMKSAGDLHLP
                     VTGIGLLWDEGYTRQMVGVGGAIEDQYPRTARDVLRRIDARFEVTVRGKKVPLRAWRV
                     DRYLTSNLYLLEPDRDEDRWITQRLYGGGVEDRLAQEIVLGVGGVRLLTALGEKTDVF
                     HFNEGHAVFAGLELLRQQRYGGTTLSERIDRLRAHVVFTTHTPVPAGNEVHELDLMRK
                     MDADLGFTDAELEVIGGDPFSMTIAGLRLARIANGVAELHGQTAREMWSQVDGAAPII
                     SVTNGVHQPTWQDARIRAALVPDKPRERQDIELWAAHQQMKGELLAEVARRTGVHLAL
                     DQLTIGFARRAAAYKRADLILGDEAALARLFGRGIQIVYSGKAHPRDLQGKALVGRLL
                     DAARRHPRHVVFLENYDMRIGALMTRGADVWLNNPRRPLEASGTSGMKAAMNGVPNVS
                     VLDGWWPEGCVHGVTGWKIGDPDPDDDAFDEHDAARVDRRDRELLHQVLEREVLAAYA
                     QRPRWLDIMRASIAMSQWRFSSDRQVEDYVARMYAAAG"
BASE COUNT          321 a          786 c          838 g          312 t
ORIGIN      
        1 gtgtcctcgg cgcgcagctt gccggcgcgc agctcggaca gctgggcgcg gctgacaccg
       61 ggcgcgcggg cggagtagcg ctgggcatcg ccctcgatct tgagcacgac ctcgggcggc
      121 agctcggcga cccgcaccgg cgacggcggg ggcggtggga tcggcaggcg gtcgcgcgtg
      181 tccagcgggc gcgcaccgtc gaccgcggcc aggaacagcg cctggtcctc cggggatacg
      241 gggggacggc gcgacctcat tcctcattct gtactcgacg gcggggcgtg ctgccaagaa
      301 atcgcacggc accggctgac agccggcgcg atcccacggg ctctgtcccc ggccgccggc
      361 agagccgtcg gggcccccca ccccggtcgc gctgcgctgg acgagcgcaa cacgacgcta
      421 agcccgcgtt gtatttggca acacagggtg gctttctcgt accgtcgctt gcagcggcgg
      481 gccgggttcg gcaccgtgcg ccctggaggc agtccatcca tggtcccgtg tccccgcgtc
      541 gcgtacttct gcatggagta cgggctcgat cccgcctttg ccatctacgc cggcggcctg
      601 ggggtgctgg ccggcgatca catgaagtcc gcaggtgacc tgcatttgcc agtgacaggc
      661 atcgggcttc tgtgggacga gggctacacg cggcagatgg tcggggtcgg cggcgcgatc
      721 gaggatcagt accccaggac cgcgcgcgat gtgctgcgcc ggatcgacgc ccggttcgag
      781 gtcacggtgc gcggcaagaa ggtgccgctg cgcgcctggc gggtcgaccg ctacctgacc
      841 tcgaacctct acctgctgga gcccgatcgc gacgaggatc gctggatcac ccagcggctg
      901 tacgggggcg gggtcgagga ccggctggcg caggagatcg tgctcggggt cggcggcgtg
      961 cgcctgctca cggcgctcgg cgagaagacc gacgtgttcc acttcaacga gggccacgcg
     1021 gtgttcgccg ggctcgagct gctgcgccag cagcggtacg gcgggaccac cctgagcgag
     1081 cggatcgacc ggctgcgcgc ccacgtcgtg ttcaccacgc acacgccggt gccggcgggc
     1141 aacgaggtcc acgagctcga cctgatgcgc aagatggacg ccgacctcgg gttcaccgat
     1201 gccgagctcg aggtgatcgg cggcgatccg ttctcgatga cgatcgccgg gctgcggctg
     1261 gcgcggatcg ccaacggcgt cgccgagctc cacggccaga ccgcgcgcga gatgtggagc
     1321 caggtcgacg gcgccgcacc gatcatcagc gtgaccaacg gggtgcatca gccgacctgg
     1381 caggatgcgc ggatccgcgc cgcgctggtg cccgacaagc cgcgcgagcg ccaggacatc
     1441 gagctgtggg ccgcgcacca gcagatgaag ggcgagctgc tcgccgaggt cgcccggcgc
     1501 accggcgtcc acctcgcgct cgaccagctc acgatcggct tcgcgcggcg cgccgcggcc
     1561 tacaagcgcg ccgacctgat cctcggcgac gaggccgcgc tggcgcggct gttcggccgc
     1621 gggatccaga tcgtctattc cggaaaggcg cacccgcgcg acctgcaggg caaggcgctg
     1681 gtcggccggc tgctcgacgc cgcccggcgc caccctcgcc acgtcgtgtt cctcgagaac
     1741 tacgacatgc ggatcggcgc gctgatgacg cgcggcgcgg acgtctggct caacaacccg
     1801 cggcggccgc tcgaggcgtc cgggacctcg gggatgaagg cggcgatgaa cggcgtgccc
     1861 aacgtctcgg tgctcgacgg ctggtggccg gagggctgcg tccacggcgt caccggctgg
     1921 aagatcggcg atcccgaccc cgacgacgac gcgttcgacg agcacgacgc cgcgcgggtc
     1981 gacaggcgcg accgcgagct gttgcaccag gtgctggagc gcgaggtgct cgcggcctat
     2041 gcccagcggc cgcgctggct cgacatcatg cgcgccagca tcgcgatgtc gcagtggcgg
     2101 ttctcgtcgg accgccaggt cgaggactac gtcgcccgga tgtacgccgc ggcgggctga
     2161 ccgctagtcg gggcggtaca gcacctggcg gcgcttgccg cgccactcca gcacgaggaa
     2221 gtcgccgcgc tgctcggcgg tggcctcgtc gaggctg
//
LOCUS       DASVOR010001101         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_303452,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001101 DASVOR010000000
VERSION     DASVOR010001101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303452"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..1432
                     /locus_tag="VF516_21065"
     CDS             221..1432
                     /locus_tag="VF516_21065"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8110241.1"
                     /translation="MSHARITRRRLLGAMGAAGALTVLPACKRSGGDAGGGGAARAVK
                     IGLVVPQSGVYATVGNEIKHGWELWLERHGGKLGNREVTTVVADEGETPQTGVPAVQK
                     LLQADQVDAIVGIISSAIALGAKDVVAEAKKLMIVANAGAGAITGAAKNPYIWRTSFT
                     NAQVASVMGKHLAGKADTGPVYLIAPDYAAGAEVIRGFKTAFEAGGGKIAGEAKPAFG
                     KTQDYQPFLTGIQSSGAKATFCFFAGAEAIAFVKQYAQFGLSASIPLYGSGFLTEGSA
                     VLAAQGDAAVGIQTTLHYSPELDNPANKEFVAAWKAKYKDSPAVYAVQGWDAASVLNR
                     ALAATTALDGESLAKAMGSVGTIDDSPRGPWSFDGQSPKQKMYLRKVEKRGADYVNTV
                     VADLGETPPIA"
     gene            1488..>1704
                     /locus_tag="VF516_21070"
     CDS             1488..>1704
                     /locus_tag="VF516_21070"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110242.1"
                     /translation="MPAWIDAHFISLLNGLAIGALLFILAVGLSLIFGMMDVLNLAHG
                     VLFLAGAYALWWIGGASPSWPAFLAALG"
BASE COUNT          273 a          588 c          613 g          230 t
ORIGIN      
        1 cccgccgcac gcacccgccg cccacgcccc cgccgcccac gcgcctgctg gccggtcggg
       61 gaactcggtg tctcggaaat acattgcaga tcgcccggag cgcgaatgag gcgggaacga
      121 gttgcgggaa cgagcacttg acacccagcg cgggcacgaa caactatgcg tcggtcgacg
      181 ccgataccgg cgttcgagac ccgtcgaccg gagggaccac atgagtcatg cgcggatcac
      241 gcggcgccgc ctgctcggcg cgatgggggc ggccggcgcc ctgaccgtat tgccggcgtg
      301 caagcgctcg ggcggcgatg ccggcggcgg cggagcggcc agggcggtca agatcggcct
      361 ggtcgtgccg cagtccggcg tctacgcgac ggtcggcaac gagatcaagc acggctggga
      421 gctatggctg gagcgccatg gcggcaagct cggcaaccgc gaggtcacca cggtggtcgc
      481 cgacgagggc gagaccccgc agaccggcgt gccggcggtc cagaagctgt tgcaggccga
      541 tcaggtcgac gcgatcgtcg gcatcatcag ctcggcgatc gcgctcggcg ccaaggacgt
      601 cgtggccgag gccaagaagc tgatgatcgt cgccaacgcc ggcgcgggcg cgatcaccgg
      661 cgcggcgaag aacccgtaca tctggcgcac gtcgttcacc aacgcccagg tcgcctcggt
      721 gatgggcaag cacctcgccg gcaaggccga caccggcccg gtctacctga tcgcgccgga
      781 ctacgcggcc ggcgccgagg tgatccgcgg cttcaagacc gcgttcgagg cgggcggcgg
      841 caagatcgcc ggcgaggcca agccggcgtt cggcaagacc caggactacc agccgttcct
      901 gaccggcatc cagtcgtccg gcgccaaggc cacgttctgc ttcttcgccg gcgccgaggc
      961 gatcgcgttc gtcaagcagt atgcccagtt cgggctgtcg gcgtcgatcc cgctctacgg
     1021 ctcggggttc ctgaccgagg gcagcgccgt gctcgccgcg cagggcgacg ccgccgtcgg
     1081 catccagacc acgctgcatt acagccccga gctcgacaac ccggccaaca aggagttcgt
     1141 cgccgcgtgg aaggcgaagt acaaggatag ccccgcggtg tatgcggtcc agggctggga
     1201 cgccgccagc gtgctgaacc gcgcgctcgc cgcgacgacg gcgctcgacg gcgagagcct
     1261 ggccaaggcg atgggatcgg tcggcacgat cgacgacagc ccgcgcgggc cgtggagctt
     1321 cgacggccag tcgcccaagc agaagatgta cctgcgcaag gtcgagaagc gcggcgcgga
     1381 ctacgtcaac acggtggtcg ccgatctcgg agagacgccg cccatcgcct gatcgcgaga
     1441 ggcccgccgg gtgaccgcgg cgctcgcggg gtagattcga cccagacatg cctgcctgga
     1501 tcgacgcgca cttcatcagc ctgctcaacg ggctggcgat cggcgcgctg ctgttcatcc
     1561 tcgcggtcgg cctgtcgctg atcttcggca tgatggacgt gctcaacctc gcgcacgggg
     1621 tgctgttcct cgccggggcg tacgcgctgt ggtggatcgg gggcgcatcg ccgagctggc
     1681 cggcgttcct cgccgcgctg gggg
//
LOCUS       DASVOR010001102         3010 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_449315,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001102 DASVOR010000000
VERSION     DASVOR010001102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3010
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449315"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1187)
                     /locus_tag="VF516_21075"
     CDS             complement(<1..1187)
                     /locus_tag="VF516_21075"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8110243.1"
                     /translation="MSIICGTDLSEASGGALDVACALAAQRDDCEVVLVHVVDPDAGE
                     PSDAELAALRARLEAQAGARRATPGPAIRTALLTGPIEQTLVGAAESEASDLLIIAAH
                     SRAERQPRLGATAAKVIALTHVPVIVVRDPAPWIAFARGTRPLKILLGIDDSATCDLG
                     VQWTLALGKRGPIDVVLGAIYYPDDAAAYYGLPARSLVETDPEIERLMERDLIRRFGG
                     PALPIARQVSARARRGLGRIGDHMIELARDEQVDAIIVGTGQKTGLGRLGSVSSVIVE
                     DAPESVVCVPPQAVVVTQAVPVLQRAVVATDLSQFANRAVPYAFALTPATGEIHVLHV
                     VKDDAEIDEAALIRQLQALAPAGARQAVHAHVVRGDDTATTIAQCAARFGVDVICIAS
                     HGRS"
     gene            complement(1239..1640)
                     /locus_tag="VF516_21080"
     CDS             complement(1239..1640)
                     /locus_tag="VF516_21080"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02266 family protein"
                     /protein_id="HEX8110244.1"
                     /translation="MGLKAKGDAAAEVPVPVDPTDAERRGTPRVLVHLEVDCASEDNY
                     LFAYITDISATGIFVRTTTPEQPGTRLHLRLSAEDAGSIEVEGDVIWVNPYRPGTPDN
                     LHPGMGIRFVNLDSAVRDRLFELIGRFAYLS"
     gene            complement(1713..>3010)
                     /locus_tag="VF516_21085"
     CDS             complement(1713..>3010)
                     /locus_tag="VF516_21085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110245.1"
                     /translation="GALTLAWAGAAWVASEAFQRKVGFTVYAGFPAMAIAVGAWLDGM
                     FAGRARGEPDAVPPGGKLIALFAVLGALELGKDLQSFSERLTSLLVGNDAIPYPAQSH
                     LIWLPTRLWVLILGMVIALGVALALAMPSDEPPRATRYRRLAARGAITAIGATFVLAA
                     FWTAIWQPALATHLSSKAMFETYQALARPGDALVVMGDLGQAPFAYTRQPPEIVPDRA
                     QIIAALGRPGRVFAIAPQSELCTLHREIAGKPYFVLDDRNLRNLLLSNRLDGATDQNP
                     LRTAIVHDEPRDIPHRPKGRVVWDGRIQLLGWDVPKSVARGARFDVTLYYKILQPVGG
                     SWKTLVHVDGPGGRAGIGDHDPIAGRCPTSTWQLGDYIIDRFTTSAGGGAFPSGTYEI
                     WTGFFTGSNPNWRNMTLSEAPGDMRDGVDRVKITTIGLE"
BASE COUNT          430 a         1054 c         1117 g          409 t
ORIGIN      
        1 gagcggccgt gcgaggcgat gcagatcacg tcgacgccga accgcgcggc gcactgggcg
       61 atcgtcgtgg cggtgtcgtc gccgcgcacg acgtgggcgt ggaccgcctg gcgggcgccg
      121 gccggggcga gcgcctggag ctgtcggatc agagcggcct cgtcgatctc ggcgtcgtcc
      181 ttgacgacgt gcagcacgtg gatctcgccg gtcgcagggg tgagcgcgaa cgcgtagggc
      241 accgcgcggt tggcgaactg cgacaggtcg gtggcgacca ccgcgcgctg cagcaccggg
      301 acggcctggg tgacgaccac cgcctggggc gggacgcaca ccaccgactc cggggcgtcc
      361 tcgacgatca ccgacgacac cgagccgagc cggcccaggc cggtcttctg gccggtgccg
      421 acgatgatcg cgtcgacctg ctcgtcgcgc gccagctcga tcatgtggtc gccgatccgg
      481 cccaggccgc ggcgggcgcg cgcgctgacc tggcgggcga tcggcagcgc ggggccgccg
      541 aaccggcgga tcaggtcgcg ctccatcagc cgctcgatct ccggatcggt ctcgaccagc
      601 gagcgcgccg gcaggccgta gtaggccgcg gcgtcgtcgg ggtagtagat cgcgccgagc
      661 acgacgtcga tcgggccgcg cttgccgagc gccagcgtcc actggacgcc gaggtcgcag
      721 gtcgccgagt cgtcgatgcc gagcaggatc ttgagcggcc gcgtgccgcg cgcgaacgcg
      781 atccacggcg ccggatcgcg gacgacgatc accgggacgt gggtcagcgc gatcaccttg
      841 gcggcggtcg cgccgagccg gggctggcgc tcggcgcgcg agtgcgccgc gatgatcaag
      901 aggtcgctgg cctcgctctc ggcggcgccg accagggtct gctcgatcgg cccggtcagc
      961 agcgcggtgc ggatcgccgg ccccggggtg gcccgccgcg cgccggcctg ggcctcgagc
     1021 cgcgcccgca gcgccgcgag ctcggcgtcg ctcggctcgc cggcgtctgg atcgacgacg
     1081 tgaaccagca cgacctcgca gtcgtcgcgc tgggcggcga gcgcgcaggc gacgtcgagc
     1141 gcgccgcccg aggcctccga cagatcggtt ccacagatga tcgacatgcg tcctacgcca
     1201 gatctacgcc aggtcttgtg ccagggctca tgccaggact aggagagata cgcgaaccgg
     1261 ccaatgagct cgaacaggcg atcgcgcacg gcgctgtcga ggttgacgaa ccggatcccc
     1321 atgccggggt gcaggttgtc cggcgtcccc gggcgatacg ggttgaccca gatcacgtcg
     1381 ccctcgacct cgatgctgcc ggcgtcctcc gccgacagcc gcaggtggag gcgggtgccg
     1441 ggctgctcgg gcgtggtcgt gcggacgaag atcccggtcg cgctgatgtc ggtgatgtac
     1501 gcgaacaggt agttgtcctc gctcgcgcaa tcgacctcga ggtggacgag cacgcgcggt
     1561 gtgccccggc gctcggcgtc ggtcggatcg accggcaccg gcacttccgc ggccgcgtcg
     1621 cccttggcct tgagacccat cgccgccgtc atagcctcaa gtcgagaccg gggtcaacgc
     1681 caagatcctg caaggtcact cgaggccgga ggtcattcga gaccgatcgt cgtgatcttg
     1741 acgcgatcga caccatcgcg catgtccccc ggcgcctcgc tgagcgtcat gttcctccag
     1801 ttcgggttcg agccggtgaa gaacccggtc cagatctcgt aggttccgct cgggaacgcg
     1861 ccgccgcccg cgctggtggt gaatcgatcg atgatgtagt cgccgagctg ccaggtcgac
     1921 gtcgggcacc ggccggcgat cggatcgtga tcgccgatgc cggcccgccc gccggggccg
     1981 tcgacgtgga ccagcgtctt ccagctgccg ccgaccggct gcaggatctt gtagtacagg
     2041 gtgacgtcga accgcgcgcc gcgcgccacc gacttcggga cgtcccagcc caggagctgg
     2101 atccggccgt cccacaccac gcggcccttg gggcggtgcg ggatgtccct gggctcgtcg
     2161 tggacgatcg cggtgcgcag cgggttctga tcggtggcgc cgtcgaggcg gttcgacagc
     2221 agcaggttgc gcaggttgcg gtcgtcgagc acgaagtacg gcttgccggc gatctcgcgg
     2281 tgcagcgtgc acagctcgct ctgcggcgcg atcgcgaaca cgcggccggg acggccgagc
     2341 gcggcgatga tctgcgcgcg gtccgggacg atctcgggcg gctggcgggt ataggcgaac
     2401 ggcgcctgac cgaggtcgcc catcacgacc agcgcgtcgc ccggccgggc cagcgcctgg
     2461 taggtctcga acatcgcttt cgacgataga tgcgtcgcca gcgcgggctg ccagatcgcg
     2521 gtccagaacg ccgcgagcac gaaggtcgcg ccgatcgcgg tgatcgcgcc gcgcgcagcg
     2581 agccggcggt accgggtggc gcgcggcggc tcgtccgagg gcatcgcgag cgcgagcgcg
     2641 acgccgagcg cgatcaccat gccgaggatc agcacccaca gccgggtcgg cagccagatc
     2701 aggtgcgact gtgccggata cgggatggcg tcgttgccga ccagcagcga ggtcagccgc
     2761 tcggagaacg actgcaggtc cttgccgagc tcgagggcgc cgagcaccgc gaacagcgcg
     2821 atcagcttgc cgcccggcgg caccgcgtcc ggctcgccgc gcgcgcgacc ggcgaacatg
     2881 ccgtcgagcc acgcgccgac cgcgatcgcc atcgccggga acccggcgta cacggtgaac
     2941 ccgaccttgc gctggaacgc ctcgctggcg acccacgccg cgccggccca cgcgagcgtg
     3001 agcgcgccga
//
LOCUS       DASVOR010001103         2432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_537190,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001103 DASVOR010000000
VERSION     DASVOR010001103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537190"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1408
                     /locus_tag="VF516_21090"
     CDS             77..1408
                     /locus_tag="VF516_21090"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8110246.1"
                     /translation="MNRTVDDVSSITEDAAPDPAIVMVASGPTAASLVLPAPAELGRR
                     IAAGDRTHEVDDERMSRDHAVVSHERGLWVIADRDSRNGTFVDGERVQGEVRRRGDVV
                     LRLGHTVFVLVADGRGQGGAQPDDQVVGPELARVYDQVRRHATSDTLLIHGESGSGKE
                     LAARLYHAAGPRAGGRFEPINCAAIPEGVAERLLFGSKKGAFSGAIDAAGHFQMAHGG
                     TLFLDEIADLDPAVQAKLLRVIETREVVPVGATNGTPVDIGIVAASHRDLRQAVAERR
                     FRDDLYFRLALATVHIPPLRARKVDIARLVQREVGSAGRKLAPHAKLIEACCLRPWPG
                     NIRELRSAVRQAAAEALAAGRDVVRVEDLAPTAGMPLMPAASEPGTGTGTAPAVERPK
                     GVVGEIDREAVVAALGRANNVVSVAARALGLHRTQLYRLMERYGISRPEDS"
     gene            1464..2150
                     /gene="nthA"
                     /locus_tag="VF516_21095"
     CDS             1464..2150
                     /gene="nthA"
                     /locus_tag="VF516_21095"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: protein motif:HMM:TIGR01323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HEX8110247.1"
                     /translation="MTDHDDDHDHGQDHDHGHGHHHGIDDPAHPHNAPLTDVQLRVRA
                     LESLLTEKGLVDPAALDTLVDTYETKVGPRNGALVVAKAWTDPAFKQRLLTDATGALA
                     DLGFIGRQGEDMVVVENTATVHHLVVCTLCSCYPWPVLGLPPVWYKSAPYRARAVIDP
                     RGVLREFGVELPDDVEVRVWDSTAELRYLVLPRRPEGTEGMTEQELAALVTRDAMIGM
                     AVVRPPEARR"
     gene            2147..>2432
                     /locus_tag="VF516_21100"
     CDS             2147..>2432
                     /locus_tag="VF516_21100"
                     /inference="COORDINATES: protein motif:HMM:NF045268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110248.1"
                     /translation="MNGAHDLGGMHGFGPVQAEPDEPVFHADWERRAFALTLAMGAWR
                     RWNLDMSRSAREQMAPAEYLATTYYEHWLFGLLRLLVEHGFASPDEIARVE"
BASE COUNT          342 a          840 c          945 g          305 t
ORIGIN      
        1 gtggccaagt atgccaggcg ttccgccccc gaacaacgaa gccacgcgcg ggcgcgcggt
       61 gttaccctcg ggctgcgtga accgcacggt cgacgacgtc tccagcatca ccgaggacgc
      121 ggcgccggac ccggcgatcg tgatggtggc gtcggggccg accgcggcat cgctcgtgct
      181 gccggcgccc gcggagctcg ggcggcggat cgcggccggc gaccggacgc acgaggtcga
      241 cgacgagcgg atgtcgcgcg atcacgcggt ggtgagccac gagcgcgggc tgtgggtgat
      301 cgcggatcgc gacagccgga acggcacgtt cgtcgacggc gagcgcgtgc agggcgaggt
      361 ccgccggcgc ggcgacgtcg tgctgcgact cggtcacacg gtgttcgtgc tggtcgccga
      421 cggccgcggc cagggcgggg cgcagccgga cgaccaggtc gtcggcccgg agctggcgcg
      481 ggtctacgac caggtccgcc gccacgcgac gagtgacacg ctgctgatcc acggcgagtc
      541 gggctcgggc aaggagctgg cggcgcggct gtaccacgca gccgggccgc gcgccggcgg
      601 ccggttcgag ccgatcaact gcgcggcgat ccccgagggg gtcgccgagc ggctgctgtt
      661 cggatcgaag aagggcgcgt tctcgggggc gatcgacgcc gccgggcact tccagatggc
      721 gcacggcggg acgctgttcc tcgacgagat cgccgatctc gatcccgcgg tgcaggccaa
      781 gctgttgcgg gtgatcgaga cccgcgaggt cgtgccggtc ggcgcgacca acggcacgcc
      841 ggtcgacatc gggatcgtcg cggcgagcca ccgcgatctg cgccaggcag tcgccgagcg
      901 ccggttccgc gacgacctgt acttccggct ggcgctcgcg acggtgcaca tcccgccgct
      961 gcgcgcgcgc aaggtcgaca tcgcgcggct ggtccagcgc gaggtcggca gcgccggtcg
     1021 caagctcgcc ccgcatgcca agctgatcga ggcgtgctgc ctgcggccgt ggccgggcaa
     1081 catccgggag ctgcgcagcg cggtgcgcca ggccgccgcc gaggcgctgg ccgcggggcg
     1141 cgacgtggtg cgcgtcgagg acctcgcacc gaccgccggg atgccgctga tgccggccgc
     1201 cagcgagccc ggcaccggga ccggaaccgc ccccgcggtc gagcggccca agggggtcgt
     1261 cggggagatc gaccgcgagg ccgtggtggc ggcgctcggc cgcgccaaca acgtggtcag
     1321 cgtggcggcc cgcgcgctcg gcctgcatcg cacccagctg taccggttga tggagaggta
     1381 cggtatctcg cgaccggaag acagctgagc cgcggcgcga ggacgcccgg cgcgatcggc
     1441 ggtaggatac gcgcgaggcg cgcatgaccg atcacgatga cgatcatgat cacgggcagg
     1501 atcatgatca cgggcacggc caccaccacg gcatcgacga cccggcgcac ccgcacaacg
     1561 cgccgctgac cgacgtccag ctgcgggtcc gggcgctgga gtcgctgctc accgagaagg
     1621 ggctggtcga cccggccgcg ctcgacacgc tggtcgacac ctacgagacc aaggtcgggc
     1681 cgcgcaacgg ggcgctggtc gtcgcgaagg cgtggaccga tccggcgttc aagcagcgac
     1741 tgctgacgga cgcgaccggc gcgctcgccg acctcgggtt catcgggcgc cagggcgagg
     1801 acatggtggt ggtcgagaac acggccaccg tgcaccacct cgtggtgtgc acgctgtgct
     1861 cctgttatcc ctggccggtg ctggggctgc cgccggtctg gtacaagtcg gcgccgtacc
     1921 gcgcacgggc ggtgatcgat ccgcgcggcg tgctgcgcga gttcggcgtc gagctccccg
     1981 acgacgtcga ggtccgggtg tgggactcga cggccgagct gcgctacctg gtgctgccgc
     2041 ggcggcccga ggggaccgag ggcatgaccg agcaggagct cgcggcgctg gtcacccgcg
     2101 acgcgatgat cggcatggcc gtggtgcggc cgccggaggc gcgacgatga acggcgcgca
     2161 cgacctcggc ggcatgcacg gcttcggccc ggtgcaggcc gagcccgacg agccggtgtt
     2221 ccacgccgac tgggagcgcc gggcgttcgc gctgaccctg gcgatgggcg cctggcggcg
     2281 ctggaacctc gacatgagcc gctcggcgcg cgagcagatg gcgccggccg agtacctggc
     2341 gaccacctat tacgagcact ggctgttcgg cctgctgcgg ctgctggtcg agcacggctt
     2401 cgcatctccc gacgagatcg cgcgagtcga gc
//
LOCUS       DASVOR010001104         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_419664,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001104 DASVOR010000000
VERSION     DASVOR010001104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419664"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="VF516_21105"
     CDS             complement(<1..387)
                     /locus_tag="VF516_21105"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8110249.1"
                     /translation="MRVESLLAFCVAACSASTPAPPPADLALTASAARVAPGSQAFFT
                     ATRDGRYLLDAAWESSDPSVLSIAGRSDGTATVTGVAPGIATITATAPEGSGAASLEV
                     ADRRVTGIAVQVGAGQVAAGRDLPVTA"
     gene            complement(474..2744)
                     /locus_tag="VF516_21110"
     CDS             complement(474..2744)
                     /locus_tag="VF516_21110"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEX8110250.1"
                     /translation="MGRKEDALEYHSRGRRGKLEVVPTKPLVSQLDLSLAYSPGVAEP
                     CLEIARDQDRSWEYTARANLVAVISNGTAVLGLGDIGPHAGKPVMEGKACLFKKFADI
                     DVFDLEINEKDVDKFCDVVAALEPTFGGINLEDIAAPACFEIEEKLRQRMRIPVFHDD
                     QHGTAIISGAGLINAALLAGKKLEAIKLVVSGAGAAAIACVEFFCSLGMKAEHVVLVD
                     SKGVVHRGRKDLTRQKARWAVPDDGRRTLADAMRGTDMFMGLSIANVVTPDMLRTMND
                     RPIVFALANPDPEISYPDAIMARPDALVATGRSDFPNQVNNVLGFPYIFRGALDCRAT
                     GVSEAMKLAAARALADLTREPVPDSVVMAYGGKSLKMGPDYFIPKPFDPRVLWWVAPA
                     VARAAMESGVARISFDVNEYRERLVSRGSNAAYSIMRTITKEAQRDPRRIVFPHAANA
                     RLLRAVQQIVDAGTARPILLGRSAEIEKLCTEMSLDLMSRVEVIDPRTAPPQRYVERL
                     YELRQRKGLTEFAAQALITNSDYYASIMVDRGDADGMVTGLRLNYPETVRPPLEIFGL
                     SPGAKVAVGMYMMVQQNSVMFFGDTVFNIEPDADTLADITVQMADAVQGFGVTPRVAM
                     ISYSNFGSVRHPDVTRIHRALELIRERRPDLEIDGEMQPEVALNPALRHQHYGFSRLT
                     QPANTLVFNSLAAGNAAYQIARAVAGASAVGPILLGLSKPVAAMQNDATVEEIVNMTS
                     HVVLKAQQMHRSQVRA"
BASE COUNT          460 a          975 c         1269 g          480 t
ORIGIN      
        1 cgctgtcacc gggagatcgc ggccggcagc gacttgcccg gcgccgacct gcacggcgat
       61 cccggtgacc cggcggtcgg cgacctcgag cgacgccgcg ccggatcctt cgggtgcggt
      121 cgccgtgatc gtcgcgatgc cgggcgcgac gccggtcacc gtcgccgtgc catcgctgcg
      181 gcccgcgatg ctcaacaccg atgggtcgct ggactcccag gcggcgtcga ggaggtagcg
      241 gccgtcgcgg gtcgcggtga agaacgcctg gctgcccggc gcgacccggg ccgcgctcgc
      301 ggtcagcgcc agatctgccg gcggcggcgc cggcgtggac gcgctgcacg cggcgacaca
      361 gaacgcgagg agcgactcga cccgcacgcg cagactttac cagagctcgc gcgactcccg
      421 gccgcgaagg ctcgcttgcc agggtctgcc ggaccgcgcg gctggccctc ctgctacgcg
      481 cggacctggc tgcggtgcat ctgctgggcc ttgagcacga cgtgcgaggt catgttgacg
      541 atctcctcga cggtggcgtc gttctgcatg gcggcgaccg gcttggacag gccgagcagg
      601 atcgggccga cggcggaggc gccggcgacc gcgcgggcga tctggtaggc ggcgttgccg
      661 gcggcgagcg agttgaacac cagggtgttg gccggctggg tcaggcgcga gaagccgtag
      721 tgctggtggc ggagggcggg gttgagcgcg acctcgggct gcatctcgcc gtcgatctcg
      781 aggtcggggc ggcgttcgcg gatgagctcg agagcgcggt ggatgcgggt gacgtccggg
      841 tggcggaccg agccgaagtt ggagtaggag atcatcgcga cgcgcggggt gacgccgaag
      901 ccctggacgg cgtcggccat ctgcacggtg atgtcggcga gggtgtcggc gtcgggttcg
      961 atgttgaaca cggtgtcgcc gaagaacatg accgagttct gctggaccat catgtacatg
     1021 ccgacggcga ccttggcgcc cggcgacagg ccgaagatct cgagcggcgg acggacggtc
     1081 tcggggtagt tgagccggag cccggtgacc atgccgtcgg cgtcgccgcg atcgaccatg
     1141 atcgaggcgt agtagtcgga gttggtgatc agcgcctggg cggcgaactc ggtgaggccc
     1201 ttgcgctggc ggagctcgta gagccgctcg acgtagcgct ggggcggggc ggtgcgcgga
     1261 tcgatgacct cgacgcgcga catcaggtcg agcgacatct cggtgcacag cttctcgatc
     1321 tcggcggagc ggccgagcag gatcgggcgg gcggtgccgg cgtcgacgat ctgctggacg
     1381 gcgcggagca ggcgggcgtt ggcggcgtgg gggaacacga tgcgccgcgg gtcgcgctgg
     1441 gcctccttgg tgatcgtgcg catgatcgag taggcggcgt tggagcccct ggagaccagg
     1501 cgctcgcggt actcgttgac gtcgaagctg atgcgcgcga cgccggactc catggcggcc
     1561 ctggcgaccg cgggggcgac ccaccacagg acgcgcgggt cgaacggctt ggggatgaag
     1621 tagtccgggc ccatcttgag cgacttgccg ccgtaggcca tgacgacgga gtcggggacc
     1681 ggctcgcggg tgagatcggc gagcgcgcgg gcggcggcga gcttcatggc ctcggacacg
     1741 ccggtggcgc ggcagtcgag cgcgccgcgg aagatgtagg ggaagccgag gacgttgttg
     1801 acctggttgg ggaagtcgga ccggccggtg gcgaccaggg cgtcggggcg cgccatgatc
     1861 gcgtcggggt acgagatctc cgggtcgggg ttggcgagcg cgaagacgat cgggcgatcg
     1921 ttcatggtgc gcagcatgtc gggggtgacg acgttggcga tcgacaggcc catgaacatg
     1981 tcggtgccgc gcatggcgtc ggcgagggtg cggcggccgt cgtcggggac cgcccagcgc
     2041 gccttctggc gggtcaggtc cttgcggccg cggtggacga cgcccttgga gtcgacgagg
     2101 acgacgtgct cggccttcat gccgaggctg cagaagaact cgacgcaggc gatcgcggcg
     2161 gcgccggcgc ccgacacgac gagcttgatc gcctcgagct tcttgccggc gagcagggcg
     2221 gcgttgatca ggccggcgcc cgagatgatc gcggtgccgt gctggtcgtc gtggaacacg
     2281 gggatccgca tgcgctggcg cagcttctcc tcgatctcga agcacgccgg cgcggcgata
     2341 tcttcgaggt tgatcccgcc gaacgtcggc tcgagcgcgg cgacgacgtc gcagaacttg
     2401 tcgacgtcct tctcgttgat ctcgagatcg aacacgtcga tgtcggcgaa cttcttgaac
     2461 aggcaggcct tgccctccat caccggcttg ccggcgtgcg ggccgatgtc gccgaggccg
     2521 agcacggcgg tgccgttgga gatcacggcg accaggttgg cgcgcgcggt gtactcccac
     2581 gagcggtcct ggtcgcgtgc gatctccagg catggctcgg cgacgccggg actgtaggcg
     2641 agcgacagat cgagctgact gaccagcggc ttggtcggta caacctcgag ctttcctcgg
     2701 cggccacggc tgtgatattc gagcgcgtct tcctttcgcc ccatgcagcg cacggtagac
     2761 ctgtcgcgca cggctggcaa atcacacgag ctgggaggca cagggtcctc gaaaatccgc
     2821 ggatcagcgg ttctggactt ggtgggcaat cacaccgtcg gagacgcgcg ctgcgacgag
     2881 gtcggtgtag gcggcgcgct gtttcatgaa tcgctgacag cgatgtcgcc cggcgcacgt
     2941 tgctgtcgga atgccgacag caacgtcccg cggaggggcc gcggaggggc ggcggagggg
     3001 cggcggaggg gcgcaggagc gcggcgatgg cgcgccgcag attctgcgca cccgcgcgcg
     3061 gcgtggacgc gaacgtcacg cggaggctgg tgaccgatgc gagcaggccc ggggagcgca
     3121 gggcggccgc ggaggctgtc gtcgagcgaa gcgagatggg ggtggggacc ctgccggctc
     3181 tgcc
//
LOCUS       DASVOR010001105         1816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_273473,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001105 DASVOR010000000
VERSION     DASVOR010001105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1816
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273473"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1204)
                     /locus_tag="VF516_21115"
     CDS             complement(<1..1204)
                     /locus_tag="VF516_21115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110251.1"
                     /translation="MANDDQRGAAWVATVSAVIVIASFVASKAARDAILLARFDITSL
                     PLFLAISAVTSLPIILVAGRLMVRWGPARLIPVLNIVSAALAVGEWLLIGRAPRVISV
                     AVFFHLATLGAVLVSGFWSIVNERFDVQSAKRHIARIGIGATLGGIFGGVIAERSAVY
                     LAPDAILLVLAGLQLACGVTLRLFGGSERRAPPAAAPHESWLALRAATRSPLLRILGG
                     LVILGAIAAAVMDYIFKADIVRGASHDGVLRSLAIFYTVTSVITAVVQVAVCGPLIAR
                     LGVARSVATLPATITVFGIAALAMPIPVVAAVARGAEAVTRNSVYRAGYELIYAPLVE
                     DHKRPAKLVLDVGADRIGDVLGAQLIGLLVYALAEPRTGLLIATVCVGAAAVVLALRL
                     PRSYTMALE"
     gene            complement(1194..>1816)
                     /locus_tag="VF516_21120"
     CDS             complement(1194..>1816)
                     /locus_tag="VF516_21120"
                     /inference="COORDINATES: protein motif:HMM:NF015194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HEX8110252.1"
                     /translation="PGPAAPAAAPAAAGKPASGDPLAAVDSAQGRYLESFYSDYYALV
                     NLLSFVLQAVVVVRLMTWLGIRRALFVMPLIVLGGWIAVGLFVNVAMVRTEKTAENSL
                     DYSLHNTLRQALFLPTSRDSKYKAKAAIDTFFFRMGDVIAGLGIVFLFVEVLGLGIRA
                     FAVLNIVLAVCWIALAARAGRLHDRLAVAGGEATAGERSRAGRHGE"
BASE COUNT          304 a          676 c          639 g          197 t
ORIGIN      
        1 cctcgagcgc catcgtgtag ctgcgcggca gccggagcgc gagcacgacc gcggccgcgc
       61 cgacgcagac cgtggcgatc aagaggccgg tccgcggctc ggccagcgcg tacaccagca
      121 ggccgatcag ctgcgcgccg agcacgtcgc cgatccgatc ggccccgacg tcgagcacga
      181 gcttggcggg tcgcttgtgg tcctcgacca gcggcgcgta gatcagctcg tagccggcgc
      241 gatacaccga gttgcgggtc accgcctcgg cgccgcgcgc gaccgccgcg accaccggga
      301 tcggcatggc gagcgccgcg atcccgaaca cggtgatcgt cgccggcagc gtcgcgacgc
      361 tgcgcgccac cccgagccgc gcgatcagcg gaccgcacac ggcgacctgg acgaccgcgg
      421 tgatcacgct ggtcacggtg tagaagatcg ccagcgatcg cagcacgccg tcatgcgacg
      481 cgccgcgcac gatgtcggcc ttgaagatgt agtccatgac cgccgcggcg atcgcgccga
      541 ggatgaccag gccgccgagg atgcgcagca gcggagaccg ggtcgcggcg cgcagcgcga
      601 gccaggattc gtggggcgcg gcggcgggcg gcgcgcggcg ctcggacccg ccgaacagcc
      661 ggagcgtgac cccacaggcc agctgcagcc cggcgagcac cagcaggatc gcatcgggcg
      721 ccaggtacac cgcggagcgc tccgcgatca cgccgccgaa gatcccgccc agcgtcgcgc
      781 cgatgccgat ccgcgcgatg tggcgcttcg cgctctggac gtcgaaccgc tcgttgacga
      841 tcgaccagaa ccccgagacc agcaccgccc ccagcgtcgc gaggtggaag aacacggcca
      901 ccgagatcac gcgcggcgcg cgtccgatca gcagccactc gccgaccgcg agcgccgcgc
      961 tcacgatgtt gagcaccggg atcagccgcg ccggccccca ccgcaccatc agccggccgg
     1021 cgaccaggat gatcggcagc gaggtcaccg ccgagatcgc gaggaacagc ggcaggctgg
     1081 tgatgtcgaa ccgggcgagc aggatcgcgt cgcgcgccgc cttgctggcg acgaacgacg
     1141 cgatcacgat caccgcgctg accgtcgcga cccacgccgc gccgcgctgg tcgtcattcg
     1201 ccatggcggc cggcccgtga ccgctccccg gcggtcgcct cgccgcccgc caccgcgagc
     1261 cggtcgtgca accggccggc tcgcgcggcc agcgcgatcc agcacaccgc gaggacgatg
     1321 ttcagcacgg cgaacgcgcg gatgccgagc ccgaggacct cgacgaacag gaacacgatg
     1381 cccaggcccg cgatcacgtc ccccatgcgg aagaagaacg tgtcgatcgc ggccttcgcc
     1441 ttgtacttgc tgtcgcggct ggtcggcagg aacagcgcct ggcgcagcgt gttgtgcagc
     1501 gagtagtcga ggctgttctc cgcggtcttc tcggtccgga ccatcgcgac gttgacgaac
     1561 aggccgacgg cgatccagcc gccgagcacg atcagcggca tgacgaacag cgcgcgccgg
     1621 atccccagcc aggtcatcag ccggaccacg accacggcct ggagcacgaa cgacagcagg
     1681 ttcaccagcg cgtagtagtc ggagtagaac gactcgagat agcggccctg ggcggagtcg
     1741 accgccgcga gcgggtctcc ggacgccggc ttccccgccg ccgccggcgc cgcggccggg
     1801 gccgccggac cggggg
//
LOCUS       DASVOR010001106         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_566765,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001106 DASVOR010000000
VERSION     DASVOR010001106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_566765"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="VF516_21125"
     CDS             <1..397
                     /locus_tag="VF516_21125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110253.1"
                     /translation="GGAVASAAGAPAEAVDPAPDAVELRHEFRGAVQSIHVEQDTESV
                     VTYQFTDAAGRPIGAPASSACRRFMISKAYPGATGVRVTHAPGRAPRVYEVFDRAHFP
                     TVDLTTAVSPSDERPLPIHIRRTIGRTGP"
     gene            394..1077
                     /locus_tag="VF516_21130"
     CDS             394..1077
                     /locus_tag="VF516_21130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110254.1"
                     /translation="MTRLSRFALRRLLSRIIGQPGPRDAMLRFVYYTEEFVERYDIQP
                     ILPHLGEAERDAMLRHFRDESAHARALRAYCRGHGLAIERSPAEDALIRRSDEGYAQY
                     LRHLDPATHRFTPEDMYAYYCHVHVQEELANDLYSAVADTLEQHREHPKLVKILRAFA
                     KSEVKHQDYAGEFMKSYEARLGARHCRRVLRRTRIDSARTGLKFFRDFLRLLVSEHQF
                     QPGALAVVL"
     gene            1080..>1846
                     /locus_tag="VF516_21135"
     CDS             1080..>1846
                     /locus_tag="VF516_21135"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110255.1"
                     /translation="MPLNWAVSALRARTIRRRVAGSGWRDAKNLPVIYVPGILGVKLF
                     DRTHRLDLWGDARGILVPGPKRRPFALDDSDDVVATETLHHFPIASRLVTTLVTAELV
                     DTLETALGYREGHDLIFLAHDWRRDYRRVAERLELEIRRIRLDFGARQRVVIIGQSVA
                     NLAVRLMLRTCAPEIRASVARWYAFGPPWRGTFNALKMLREGYYPASRRFKGFTPADA
                     ATFAPCYQLLPRGAALLDERGAPVAGFDLYDAASWIE"
BASE COUNT          255 a          700 c          627 g          264 t
ORIGIN      
        1 cggcggcgcg gtcgcgagcg cggcgggcgc acccgcggag gccgtggacc cggcgccgga
       61 cgccgtcgag ctgcgccacg agttccgcgg cgcggtgcag tcgatccacg tcgagcagga
      121 caccgagtcg gtcgtgacct accagttcac cgacgccgcg ggccggccga tcggcgcgcc
      181 cgcgagctcg gcgtgccggc ggttcatgat ctcgaaggcg taccccgggg cgaccggcgt
      241 gcgggtcacc cacgcgccgg gccgtgcgcc gcgcgtctac gaggtgttcg accgcgcgca
      301 cttcccgacg gtcgacctca ccaccgccgt gtcgccgagc gacgagcgcc cgctgccgat
      361 ccacatccgg aggaccatcg ggaggaccgg gccatgacac ggctgtctcg cttcgccctg
      421 cggcggctcc tgtcgcggat catcggccag cccggcccgc gcgatgccat gctgcgcttc
      481 gtctactaca ccgaggaatt cgtcgagcgc tacgacatcc agccgatcct cccccacctc
      541 ggcgaggccg agcgcgacgc gatgctgcgc cacttccgcg acgagtcggc gcacgcgcgc
      601 gcactccgcg cctactgccg gggccacggc cttgcgatcg agcgctcgcc cgccgaggac
      661 gcgctgatcc ggcgctcgga cgaaggctac gcccagtacc tccgccacct cgatccggcg
      721 acccaccggt tcacccccga ggacatgtac gcgtactact gtcacgtcca cgtgcaggag
      781 gagctcgcca acgatctgta cagcgcggtc gccgacaccc tcgagcagca tcgcgagcac
      841 ccgaagctgg tcaagatcct gcgcgcgttc gcgaaaagcg aggtcaagca tcaggactac
      901 gccggcgagt tcatgaagtc ctacgaggcg cggctcggcg cgcggcactg ccggcgcgtg
      961 ctccggcgga cccggatcga cagcgcgcgg accgggctca agttcttccg cgacttcctg
     1021 cggctgctcg tgtcggagca ccagttccag cccggcgccc tcgccgtcgt gctctagcga
     1081 tgccgctgaa ctgggccgtc tcggcgctcc gcgcccgcac catccggcgg cgggtcgccg
     1141 ggtccggctg gcgcgatgcg aagaacctgc cggtcatcta cgtgcccggc atcctcggcg
     1201 tgaagctgtt cgatcgcacg caccgcctcg atctgtgggg agacgcgcgc ggcatcctgg
     1261 tcccgggccc gaagcgccgg ccgttcgcgc tcgacgactc cgacgacgtc gtggcgaccg
     1321 agacgctgca tcacttcccg atcgcgtcgc ggctggtcac gacgctggtg accgccgagc
     1381 tggtcgacac gctcgagacc gcgctcggct accgcgaggg ccacgacctg atcttcctcg
     1441 cccacgactg gcggcgcgac taccggcgcg tcgccgagcg gctcgagctc gagatccggc
     1501 ggatccggct cgatttcggc gcgcgccagc gcgtcgtgat catcggccag tcggtcgcga
     1561 acctcgcggt gcggttgatg ttgcgcacgt gcgcgccgga gatccgcgcg agcgtcgcga
     1621 gatggtacgc gttcggaccg ccgtggcgcg gtacgttcaa tgcgctcaag atgctgcgcg
     1681 agggctacta cccggcgagc cgccggttca agggcttcac gcccgccgac gccgccacgt
     1741 tcgcgccgtg ctaccagctc ctgccgcgcg gcgccgcact gctcgacgag cgcggcgcgc
     1801 cggtcgccgg cttcgatctg tacgacgccg cctcctggat cgagca
//
LOCUS       DASVOR010001107         2966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_303500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001107 DASVOR010000000
VERSION     DASVOR010001107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VF516_21140"
     CDS             <1..498
                     /locus_tag="VF516_21140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8110256.1"
                     /translation="SPPKALPVVTFTTDITLHFNGEDIHVIHVDPTHTDGDSIIVFPK
                     SKVIHMGDTYMTISYPFADISSGGNFDGFIATSDKVVGMSDATTKIIPGHGPLSSKAE
                     LKAWHDMLVEVRAAVKKLADAGKSLDAIKQAKPTAKWDEKWGGKFIKGDQVVEFAFAA
                     IKGKH"
     gene            complement(618..1892)
                     /gene="tyrS"
                     /locus_tag="VF516_21145"
     CDS             complement(618..1892)
                     /gene="tyrS"
                     /locus_tag="VF516_21145"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEX8110257.1"
                     /translation="MLILDELDARGLLADVTDRDGLAALLASEIVTVYAGYDPTSPSL
                     HVGNLIPTILLRRFQCAGHRPIVLVGGATGMVGDPSGKSTERNLMDDETLAVNLAGIR
                     AQLARLLDFEDGKTGAIMTNNADWTRGVGLLEFLRDYGKHLTVNYMLAKESVGARISS
                     ESGISYTEFSYMLLQAFDFVQLHRLHGCRLQVGGSDQYGNITAGCELQHKLRGPRLFG
                     LVAPLLLDPTGAKMGKTSTGERIWLDPERTSPYAFYQYWLNRSDEETPGLLRIFSLRP
                     LGEIEELVREHDRDRSRRSAQRELARTLTAWIHGPAIVPRVEVASRVMFGDSLDGLTD
                     ADLAVLAGTVPTVDVARSELASGVGIVDLLARTISDSKGAARRLITQGGAYINNQRVT
                     EVEHQVTLAQLATETMLVVRGGKKDYCLVRVT"
     gene            2161..>2966
                     /locus_tag="VF516_21150"
     CDS             2161..>2966
                     /locus_tag="VF516_21150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110258.1"
                     /translation="MRGSALPRTQLFADEPVGELRGLATLTDPDDATAVIDLAVIGAR
                     GDGKTQFIVHAIRAMHAHAPALDGPEQVLNRDVLKLVLDPRATRPDATPPGVVPHFTF
                     RMRSAGLFDRLSWLGAIQLAWRATGVATALVLGAALLVLGAVVGLKLGIGPGVIVGAS
                     GLVVGSAAALAARRRIARAGDVEVAFWDVAGEQVYSAAAADYYGLLAHLVEARRRRAA
                     ELGRAYAFAPVLICNPIALGTADEGSPYEWMRQLLPLFAALDPAAARAMIA"
BASE COUNT          427 a         1004 c         1075 g          460 t
ORIGIN      
        1 tcgccgccca aggcgctgcc ggtcgtcacg ttcacgacgg acatcacgct gcacttcaat
       61 ggcgaggaca tccacgtgat ccacgtcgat ccgacccaca ccgacggcga ctcgatcatc
      121 gtcttcccca agtccaaggt cattcacatg ggggacacgt acatgacgat ctcgtacccg
      181 ttcgccgaca tcagctcggg cggcaacttc gacggcttca tcgcgacgtc cgacaaggtc
      241 gtcggcatga gcgacgcgac caccaagatc attccgggcc acggcccgct gtccagcaag
      301 gccgagctca aggcctggca cgacatgctg gtcgaggtcc gcgcggcggt caagaagctg
      361 gccgacgcgg gcaagtcgct cgacgcgatc aagcaggcca agccgaccgc caagtgggac
      421 gagaagtggg gcggcaagtt catcaagggc gatcaggtcg tcgagttcgc gttcgccgcg
      481 atcaagggca agcactgatc gaggccgtcg cggacggacg gcgtcgttcc gacggcgcct
      541 ggggtccgat cccgtcgccg cggcgctccg ggcggcgcgc gcagcgctgc cccggtggca
      601 gcgccggtct ccgccgatca ggtcacccgg accaggcagt aatccttctt cccgccgcgc
      661 accacgagca tggtctcggt cgcgagctgg gcgagggtga cctggtgctc gacctcggtg
      721 acgcgctggt tgttgatgta cgcaccgccc tgcgtgatca gccggcgcgc ggcgcccttg
      781 gagtcgctga tcgtgcgggc gagcaggtcg acgatcccca cccccgaggc cagctcgctg
      841 cgcgcgacgt cgaccgtggg cacggtgcca gcgagcacgg cgaggtcggc gtcggtcagc
      901 ccgtcgagcg agtcgccgaa catcactcgg ctcgcgacct ccacccgcgg cacgatcgcc
      961 ggaccgtgga tccacgcggt cagcgttcgc gcgagctcgc gctgcgcgct gcgccggctg
     1021 cgatcccggt cgtgctcgcg gaccagctcc tcgatctcgc cgagtgggcg cagcgagaag
     1081 atccggagta gccccggggt ctcctcatcg ctgcgattga gccagtactg gtagaacgcg
     1141 tacggcgacg tccgctcggg gtcgagccag atccgctctc cggtcgaggt cttgcccatc
     1201 ttggcgccgg tggggtcgag caggagcggc gcgaccagcc cgaacagccg gggcccgcgc
     1261 agcttgtgct ggagctcgca gcccgccgtg atgttgccgt actgatccga gccgccgacc
     1321 tggagccggc agccgtgcag ccggtggagc tggacgaagt cgaacgcctg gagcagcatg
     1381 tacgagaact ccgtgtagct gatcccggat tcggaagaga tccgcgcgcc caccgactcc
     1441 ttggcaagca tgtagttgac cgtcaggtgc ttgccgtagt cgcgcaggaa ctccaggagc
     1501 ccgacgccgc gggtccagtc cgcgttgttg gtcatgatcg cgccggtctt cccgtcctcg
     1561 aagtcgagca ggcgcgcgag ctgggcgcgg atccccgcga ggttgaccgc cagcgtctcg
     1621 tcatccatca ggttgcgctc ggtgctcttg cccgatggat cgccgaccat gcccgtcgct
     1681 ccgccgacca ggacgatcgg ccggtgcccg gcgcactgga agcgccgcag caggatggtc
     1741 gggatcaggt tgccgacgtg caagctcggg gacgtggggt cgtagccggc gtagaccgtc
     1801 acgatctcgc tcgccagcag cgcagcgagt ccatcgcggt cggtcacgtc ggccagcaag
     1861 ccacgggcat cgagctcgtc gaggatcagc atcgcgctga ccctagcgcg accgcgcagc
     1921 gcggcaaagc cttggaaagc cttggatcat ggttgccggc aggcgcctgg accctcgcgc
     1981 aaccgccgtt ccggggttcg cttgcgacgc ccattctgcg caacagccgg gacgatccgc
     2041 cccgggctga cgtcttgcca gatcctgttg ccagatcttg ccatgtgtct gtggacgcgg
     2101 gggacggcct gcccggtgcg gcatgtccac cgcggagccc ggtcgtggat ccggggtgcc
     2161 atgagaggtt ctgccctgcc gcggacgcag ctgttcgcgg atgagccggt aggtgagctg
     2221 cgcggcctgg cgacgttgac cgatcctgac gacgcgaccg cggtgatcga tctggcggtg
     2281 atcggggcgc gcggcgacgg caagacgcag ttcatcgtgc acgcgatccg cgcgatgcac
     2341 gcgcacgcgc ccgcgctcga tggcccggag caggtgctca accgcgacgt gctgaagctg
     2401 gtgctcgatc cacgcgcgac gcggccggac gccacgccgc cgggggtggt gccgcacttc
     2461 acgttccgga tgcggtccgc ggggctgttc gaccggttga gctggctcgg cgcgatccag
     2521 ctggcctggc gcgcgaccgg agtggcgacc gcgctggtgc tcggcgcggc gctgctggtg
     2581 ctcggcgcgg tggtcgggct caagctcggg atcgggccgg gagtgatcgt cggggcgagc
     2641 gggctggtgg tcggcagtgc cgcggcgctc gcggcgcgcc ggcggatcgc gcgggcgggc
     2701 gacgtcgagg tcgcgttctg ggacgtcgcc ggcgagcagg tgtactcggc ggcggcggcc
     2761 gattactacg ggttgctcgc gcatctggtc gaggcgcggc ggcggcgcgc cgcggagctc
     2821 ggccgcgcgt atgcgttcgc gcccgtgctg atctgcaacc cgatcgcgct ggggacggcc
     2881 gacgagggca gcccgtacga gtggatgcgg cagctgctgc cgctgttcgc ggcgctcgat
     2941 ccggcggctg cgcgcgcgat gatcgc
//
LOCUS       DASVOR010001108         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390758,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001108 DASVOR010000000
VERSION     DASVOR010001108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390758"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2430
                     /locus_tag="VF516_21155"
     CDS             <1..2430
                     /locus_tag="VF516_21155"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8110259.1"
                     /translation="SGELRSDVTTESRLTLASAPERLAGAAITSRRGLPVRIADVADV
                     GPGAEPPVGATACDGSPGVYVQMMKLPWADTVQTTAEVERAIDDLRRDLPAGAELHPP
                     LFRQASFVETSIRSVARAMAIGTLLVVIVLIALLRSVRLAAISLTAIPLSIVTAAAVL
                     VARGASINGMVLGGLAIAVGEVVDDAIVDVENVWRRLRLNAELPVPRPALEVVRDASR
                     EVRGSVVYATAIVCLVLVPVAVLGGVAGRIFSPLAETYLLAIVASLVVALTVTPALCA
                     IALPRIATAAARPSRIALAMAARYRRILFRVIDHPRIVVGAALIGALAAAVAVPLLGG
                     SFLPDFHEQSVIIHVNAAPGTSLDETMRLGGRFDARVRPAIARHVALRGGRAELGEDP
                     YPANRLEIDAVIGSDELDARVDALRAQLATLPGISFAIEGFLGERVHEILAGEAAPIV
                     VEVIGPELEPLRGIAATLAARISRLPDVRGVTVEPQVEIAELRIRPDPVALAPYGLTP
                     ADVAEQVHTWRMGRRVADVLEPGGRRIGLVVSGAAAMRERGALLDLPITTPDGRTVPL
                     SAVARLDDVAVPAAVSHVAGQRRIAIGVAASRSAVSQVSAAIEDRSLAASLPPGYRIA
                     IGGEAVARSHAARQLLAIGLLIVVGILVLLGSAFGRAGDAAIVLVNFPLGLIGGVAGA
                     LLLPEGLSVAGFVGFVTLFGIIARNGIMLVSHIRQLEHEAPDEPPVARVLRAAEERLL
                     PIAMTAATAGLGLLPLAISLGSAGSELEAPMAVIVCGGLVTSTLLNMIVLPTIYVWIA
                     RRAARRKEKAT"
BASE COUNT          271 a          940 c          958 g          362 t
ORIGIN      
        1 tcgggcgagc tgcgcagcga cgtcacgacc gagtcgcggc tgaccctggc cagcgccccg
       61 gagcgcctgg ccggcgcggc catcaccagc cgccgcggcc tgccggtccg gatcgcggac
      121 gtcgcggacg tcggccccgg cgccgagccg ccggtcgggg cgaccgcgtg cgacggaagc
      181 cccggggtct acgtgcagat gatgaagctg ccctgggcgg ataccgtgca gaccacggcg
      241 gaggtcgagc gcgcgatcga cgatctgcgc cgcgacctgc cggccggcgc cgagctgcat
      301 ccgccgctgt ttcgccaggc gtcgttcgtc gagacctcga tccgcagcgt cgcgcgcgcg
      361 atggcgatcg gcacgctgct cgtcgtgatc gtgctgatcg cgctgctgcg ctcggtgcgg
      421 ctggccgcga tcagcctgac cgcgatcccg ctgtcgatcg tcaccgcggc ggcggtgctg
      481 gtcgcgcgcg gcgcgtcgat caacggcatg gtgctcggcg gactcgcgat cgcggtcggc
      541 gaggtcgtcg acgatgcgat cgtcgacgtc gagaacgtgt ggcgccggct gcgcctgaac
      601 gcggagctgc cggtgccgcg ccccgcgctc gaggtcgtgc gcgacgcgtc gcgcgaggtc
      661 cgcggctcgg tggtctacgc gaccgcgatc gtgtgcctcg tgctcgtgcc ggtggcggtg
      721 ctcggcggcg tggccggccg gatcttctcg ccgctggccg agacctacct gctggcgatc
      781 gtcgcatcgc tcgtggtcgc gctcacggtc accccggcgc tgtgcgcgat cgcgctgccg
      841 cggatcgcga ccgccgccgc ccgcccgagc cggatcgccc tggcgatggc cgcgcggtat
      901 cgccggatcc tgttccgcgt gatcgaccac ccgcggatcg tggtcggcgc ggcgctgatc
      961 ggcgcgctcg ccgccgcggt cgccgtgccg ctgctcggcg gcagcttcct gccggacttc
     1021 cacgagcagt cggtgatcat ccacgtcaac gcggcgccgg ggacatcgct cgacgagacg
     1081 atgcgactcg gcgggcgatt cgacgcgcgg gtccggcccg cgatcgcgcg ccacgtcgcg
     1141 ctgcgcggcg gccgcgccga gctcggcgag gacccgtatc cggccaaccg gctggagatc
     1201 gacgcggtga tcggcagcga cgagctcgac gcgcgggtcg acgcgctgcg cgcgcagctc
     1261 gcgaccctcc ccggcatcag cttcgcgatc gagggcttcc tcggcgagcg cgtgcacgag
     1321 atcctcgccg gcgaggccgc gccgatcgtc gtcgaggtga tcgggcccga gctcgagccg
     1381 ctgcgcggga tcgccgcgac gctcgccgcc cggatctcgc gcctccccga cgtccggggg
     1441 gtcacggtcg agccccaggt cgagatcgcc gagctgcgca tccgccccga tccggtcgcg
     1501 ctggcgccct acggactcac ccccgccgac gtcgccgagc aggtccacac ctggcggatg
     1561 gggcgccggg tcgccgacgt cctcgagccc ggcggccgcc ggatcggcct cgtggtgtcg
     1621 ggagccgccg cgatgcgcga gcgcggcgcg ctgctcgatc taccgatcac gaccccggat
     1681 ggccgcacgg tgccgctgtc ggcggtcgcg cggctcgacg acgtcgcggt gcccgcggcg
     1741 gtcagccatg tcgccggcca gcggcggatc gcgatcggcg tcgccgcgtc gcggtccgcg
     1801 gtgtcccagg tcagcgcggc gatcgaggat cgctcgctcg ccgccagcct gccgcccggc
     1861 taccggatcg cgatcggcgg cgaggccgtg gcgcggtcgc acgcggcgcg ccagctgctc
     1921 gcgatcggcc tcttgatcgt cgtcggcatc ctcgtgctgc tcggcagcgc gttcgggcgc
     1981 gccggcgacg cggcgatcgt cctggtcaac ttcccgctcg gcctgatcgg tggggtggcc
     2041 ggcgcgctgc tgttgcccga ggggctgtcg gtcgccggct tcgtcggctt cgtcacgctg
     2101 ttcgggatca tcgcgcgcaa cggcatcatg ctggtctcgc acatccggca gctcgagcac
     2161 gaggcgccgg acgagccgcc ggtcgcacgc gtgctgcgcg cggcggagga gcggctgttg
     2221 ccgatcgcga tgaccgcggc gaccgccggc ctggggctgt tgccgctcgc gatctcgctg
     2281 ggctcggcgg ggagcgagct cgaagcgccg atggcggtca tcgtgtgcgg cgggctcgtg
     2341 acctcgaccc tgctcaacat gatcgtgctg ccgacgatct acgtgtggat cgcccggcgc
     2401 gccgcgcgcc ggaaggagaa agcgacgtga aaggttctgc gtggcccgtc gcggcgatcg
     2461 ccgcggccct ggcgtcgccg gcgcgcgccc agccggcgcc gctgaccctg caacaggccc
     2521 aggccgaggc c
//
LOCUS       DASVOR010001109         4409 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_303518,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001109 DASVOR010000000
VERSION     DASVOR010001109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303518"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VF516_21160"
     CDS             complement(<1..231)
                     /locus_tag="VF516_21160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110260.1"
                     /translation="MVRSADRQGPSAQLAVVVAIDTIFLHSAKAFMRSALWQPETWKS
                     DALPRQGAIVKSLHPDLPGTVEEIEADYAARCA"
     gene            270..392
                     /locus_tag="VF516_21165"
     CDS             270..392
                     /locus_tag="VF516_21165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110261.1"
                     /translation="MGNRLLTPAEPVVRPANGNAPVERRERLGGLLNFYYRRVA"
     gene            1061..1366
                     /locus_tag="VF516_21170"
     CDS             1061..1366
                     /locus_tag="VF516_21170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110262.1"
                     /translation="MTSRYFANLKAEWQTLMDEAKRHGAIRTYRLFLAPPANREDWDV
                     MLAIEVENMAALDGFGEKMAALGARVRKDTKRAGATTKLADVREILGMKLLREVALK"
     gene            1630..2511
                     /locus_tag="VF516_21175"
     CDS             1630..2511
                     /locus_tag="VF516_21175"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX8110263.1"
                     /translation="MQLLPVPRLRTNEFRPSRWAGGPRVIVDNVRAWKALDRWQPDYL
                     KSAVGDSEVSVREVNGPPRNVFQSLAAGGRISFSDYIDWVLATADDLLAVSTKYSEAS
                     DIAREVARSGLERSYYLDIKLAQLSKTLLDDTAVPAWYRTTPVDINFWCGVLGTSSGL
                     HCDVTPNCNVQVAGSKHFILFPPSQSGLLYRIPKITHCRFDPNMPDFDRFPLARSASG
                     WQCTLRPGESLYIPVGWYHQVTVVSGWAVNVNFFWPRPFPQGLATPPLWHFLLRRSWA
                     GLRRALFRSHQKPQGTH"
     gene            2515..3180
                     /locus_tag="VF516_21180"
     CDS             2515..3180
                     /locus_tag="VF516_21180"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8110264.1"
                     /translation="MRPGLIGKIAGIAKIFRSAWRRHGLIRTVRLWSSELRFDLQNRT
                     ATALDLRGGSLHSRNRSGGHEGSNPLLFAELIRHAPVDRASSVFLDFGAGKGRALLLA
                     AQHGFRKAIGVEVRADLCAIARSNITTYRRGHSPCAMEMHCVDAAVFEVPDEVNVAYF
                     FNPFGPEVMRATLAHITESLGRRPREFFVVYLHPSLEALFLEAGFRRVWREGTDGAIL
                     CQP"
     gene            complement(3371..3565)
                     /locus_tag="VF516_21185"
     CDS             complement(3371..3565)
                     /locus_tag="VF516_21185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110265.1"
                     /translation="MRTTCGERCRSTSSTIIASATTRAWANRLLTPGEPAVRPANGNA
                     PVERRERLGGLLNFYYRRAA"
     gene            3735..4232
                     /locus_tag="VF516_21190"
     CDS             3735..4232
                     /locus_tag="VF516_21190"
                     /inference="COORDINATES: protein motif:HMM:NF014430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8110266.1"
                     /translation="MWRFAGVDLTRIDAIRVTVAQTVLTEVGPDVTAFPSENHFVSWL
                     RLCPQTPISGGKPLKKRRSSMGANRVAAALRMAALGIQRSKTALGASFRRIARHKGVA
                     VAVFATARQLARLIYRMLRYGQDYVDIGEAAFEAKFQARRLAGISEAARSLGFTLTKN
                     EVAAG"
BASE COUNT          801 a         1374 c         1420 g          814 t
ORIGIN      
        1 ggcgcagcgc gcggcgtagt cggcttcgat ctcctcgacg gtccccggta ggtcggggtg
       61 cagcgacttg acgatcgcac cttgccgcgg cagcgcgtct gacttccagg tctccggctg
      121 ccacagcgcc gagcgcatga acgccttggc gctgtggagg aagatggtgt cgatcgcgac
      181 gacgacggcg agctgggccg atggcccttg acgatcagct gatcgaacca cggggcgtcg
      241 cggatcatcg cgagcgcaac caccagggcg tgggcaaccg gctgctcacg ccagccgaac
      301 cggtggtgcg accagccaac gggaatgcgc cggtcgagcg ccgcgagcgg ctgggcggac
      361 tgctcaactt ctactaccgg cgcgtcgcat gagttcgcga tcgacttctg gcacatgacg
      421 agcacgtctc gatgagacca cagcggcacc gctccagatc ttcgcgcaga ccttgcagcg
      481 gttcctcgag ctgggcagcg accttcggca ccgtcgggat caagcagccg ccgctcgcgc
      541 agccggtgtc ggcgaccagt gcggcggacg cagcagtgta gtgccaaaga ccgggatcgc
      601 accggcaact catcataatt gctgggaatt cacaaacgaa ccgtcaaacc ttgggttaga
      661 aaacgacaag gccgcgtcct cgggcctttg ctagggcttt tctcgatatt gatggggcaa
      721 cgcgacgtgt ttgatagtcg ttacaacaga ctactgaccg tgcctcgtcg agcgttcagt
      781 tctaccgtcg caatggaata agaactcttc ctctctgaga tcgacccacg ttgggcgcgc
      841 gactccgtgg tctgtcacga tcgtgtcgga gcatgtgtct gcgcttgacg tgtgttggtc
      901 acgtggtatt aagtgttctg ccatcgggca tgcacgatcg atagttcacg ctaccagcgg
      961 agaggggtga tgaatgtgcg agccagaatt tgacgatgac cgatcttact atgtcgacgg
     1021 cccggtgtgg tcgattcagt tcattcgtac gaagccaggg atgaccagca ggtattttgc
     1081 gaacctgaag gcggagtggc agacgctgat ggacgaagcc aagcggcatg gcgccatccg
     1141 cacttatcgg ctgtttctgg cccctcctgc caaccgggag gactgggatg tcatgctggc
     1201 gatcgaggta gagaacatgg ccgcgctgga tggcttcggc gagaagatgg cggcgctggg
     1261 agccagggtc cgaaaggaca ccaagcgagc cggggcaacg acgaagctgg ccgacgtccg
     1321 agagattctc ggcatgaagc tgctccggga agtggcgctg aaataagcgc ctttcgccac
     1381 cgccggtcgc aaagagtcga tcacgaaccc gagaggacga ggaggcaatc atggtgaacc
     1441 aaacgacgag gataacgagc gccgcggcaa cgaaagtcaa gacgcggacg ctggacttga
     1501 agcgcgaggt gatccgcaac cccacagatc aactgttgac gtcgcaggga tggagcctat
     1561 ggacttgcga cgaacacgag ggaaccagcg gcgcggagta aaggtagccg caagggagcc
     1621 tgacgcgtcg tgcaactgct gccggtgccg cgcctccgta cgaacgagtt ccggccgtca
     1681 cgctgggccg gcgggcctcg agtgatcgtc gacaacgtcc gagcctggaa ggccctggat
     1741 cgctggcagc cagactatct caaatccgcg gttggcgata gcgaggtttc ggtccgagag
     1801 gtcaatggtc cgccgcggaa cgttttccag agcttggcgg caggcgggcg catttcattt
     1861 tcggattaca ttgactgggt cctcgcaaca gccgatgacc ttcttgctgt ttccaccaag
     1921 tacagcgagg cttcggacat tgcacgcgag gtcgctcgga gcgggcttga acgttcgtac
     1981 tatctcgaca tcaagctggc acagctgtca aagaccctgc tcgacgatac cgctgtcccg
     2041 gcgtggtatc gtaccacacc ggtggacatc aatttctggt gcggcgtgct cggtacatca
     2101 tcggggcttc actgtgatgt cacgccgaac tgcaatgtcc aggtggcggg tagtaagcat
     2161 ttcatccttt tccctccatc gcaatccggc ctcctctatc gaattcccaa gatcacccac
     2221 tgtcgattcg atcccaacat gcccgatttc gatcgctttc cgctggcacg ttccgcctcc
     2281 ggctggcagt gcacgctgcg tcccggggag tcactctaca tccccgtggg gtggtatcac
     2341 caggtgacgg tggtctcggg atgggcggtc aacgtcaact tcttctggcc acgaccgttt
     2401 cctcagggcc tcgccacccc gcccctgtgg cattttctgc tccgaaggag ctgggccgga
     2461 ttgcgtcgag cactgttccg cagtcatcag aagccccagg gaacgcactg attgatgcgt
     2521 ccgggactga tcggcaagat cgccgggatc gcgaagatat tcaggagcgc ttggcgtcgg
     2581 catggactca tccggaccgt gcgcctgtgg tcgagcgagc tacgattcga cctccaaaac
     2641 cggacggcga cagcgctcga cctccgcggc ggctcgctgc attcgcgcaa ccgatcggga
     2701 ggacacgagg ggtccaatcc cttgctgttt gcggagctga ttcgccacgc cccggtggac
     2761 cgcgcgagca gcgtgttctt ggatttcggt gcgggcaagg gcagggcgct gctgctcgcc
     2821 gcccagcacg gtttccggaa ggcgatcggc gtggaagttc gcgccgatct ctgtgccatc
     2881 gcgaggagca acatcacgac ctaccgccgg ggccattcgc cctgcgcgat ggagatgcat
     2941 tgcgttgacg cggctgtctt cgaggttccc gacgaggtca acgtcgcgta cttcttcaat
     3001 ccgttcgggc ccgaggtcat gcgcgcaacg ctcgcccaca ttaccgagtc cctcggccgc
     3061 cgcccccggg agttctttgt cgtctatctg cacccgtctc tcgaggcact cttcctcgaa
     3121 gcaggcttca ggcgcgtctg gcgcgaggga acggatggag cgatactgtg ccagccttga
     3181 ggacgcgacg cgtaccacaa agcctcactt cgtgaagccg acgactggcg cgggtaggac
     3241 aaacaagtgg ggattcgcgc gctccccggg gatgccttga tgtgccccga ggtgggaccg
     3301 gcgaagtggc gagcatcacc ggatggcggc tgtgtcagtg ggcgtcgtgt gccagaagtc
     3361 gatcgcgaac tcatgcggcg cgccggtagt agaagttgag cagtccgccc agccgctcgc
     3421 ggcgctcgac cggcgcattc ccgttggctg gtcgcaccgc cggttcgcct ggcgtgagca
     3481 gccggttggc ccacgccctg gtggttgcgc tcgcgatgat cgtgctcgac gtactccgac
     3541 accgctcgcc gcaggtggtt ctcacccagc ggcaccagct tgctcaaaca ctcggacttg
     3601 atcgacaaca cgaaccgttc ggcgatcgaa ttcagatcgg ggctgcgcgc cggtaggcgc
     3661 aggcacttca cccctggaac ccgaccaagg agaagacgat ccgccagcgc ggcgagcaac
     3721 cgctgcggac gagcttgtgg cggttcgcgg gcgtcgatct gacgcgcatc gatgccatcc
     3781 gcgtgacggt cgcgcagacc gtgctgaccg aggttggacc cgacgtgacg gcgtttccgt
     3841 ccgagaatca cttcgtgtcg tggctccggc tgtgcccgca gacgccgatc tcgggcggca
     3901 agcccctcaa gaaacgacgg agcagcatgg gggccaaccg ggtcgcggct gcactccgga
     3961 tggccgcgct gggaatccag cgatccaaga ccgcgctcgg cgcctcgttt cggcgtatcg
     4021 ctcgtcacaa gggcgtcgcc gtggcggtct tcgcgaccgc tcgtcaactc gcccgactga
     4081 tctaccggat gctgcgctac ggccaagact acgtggatat cggggaggcg gcgttcgagg
     4141 ccaagttcca ggctcgccgc ctcgccggga tctccgaagc cgccagatcc cttggcttca
     4201 ccctcaccaa gaacgaggtc gctgccgggt aagtttcagg tctggattgg ccgggcgttg
     4261 ccccgcacat acctagaggg gatgttgtgg cgacgcccgc accagccccc ggggaaccgg
     4321 tcacgcaagc ctctgctcag cctggagcct ccgctcggcc tgggcgtcaa ggcttccctt
     4381 cggcgctccg cggccttgac tccggcctc
//
LOCUS       DASVOR010001110         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_507894,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001110 DASVOR010000000
VERSION     DASVOR010001110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507894"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VF516_21195"
     CDS             complement(<1..672)
                     /locus_tag="VF516_21195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110267.1"
                     /translation="MSNAQQIEHQPGQQQDAAAPATETADSRDGLRADLHAQILRMTP
                     ADAGALAEMIQNYPSFLGAILAVAASHMGNAAVQRAIQLAKSWKAQGRSGALSQGEVH
                     QATGDSHPLSGSEMIAALHDESDGPQKTGPAAAPRAAQAAAEPAWVAGARAYNAAHAE
                     LVDEFNELTSDVCRLDGAGKVDPQAVARWQSNHGLAADGKIGPHTVAAARKLKAKPPE
                     VAAAPQ"
     gene            complement(746..1387)
                     /locus_tag="VF516_21200"
     CDS             complement(746..1387)
                     /locus_tag="VF516_21200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEX8110268.1"
                     /translation="MVEPSLAIALFPTPIVVFDLPDMAEVNREIAAQLLAEEQTVPSW
                     QRANVGGWHSVPDLSRRPQPWFRALMRAVVDSVGAHVAALAAHAGGDALPAYRYAVTA
                     WAMIMRSGHYVITHDHGDAHWSVAYYVDAGDDLPAPSGRIAFLDPRRSGRSIPDLSLF
                     GETFDLAPRTGALVIFPGWLQHYVHAYRGERPRICVSCNLTMEVVPPATPRNP"
     gene            1591..3030
                     /locus_tag="VF516_21205"
     CDS             1591..3030
                     /locus_tag="VF516_21205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8110269.1"
                     /translation="MQDRLGHDLGGDTITSVSHGRIGELDPGVQPYLVVLAVSDDLHA
                     PSSRHLLDEIDEVRFGRGPRSATRTTIGETRVLELRIPDAWMSSQHGRLVRGPAGWVL
                     DDPGSKNGSLINGQVVRQGVVSDGALIELGHSFLLFCEQPVEHDAADDLIDGELAVLS
                     PALATFFGPLADGFAALGRLARTPVSIVVLGETGTGKEVVARALHAMSGRTGAFVAVN
                     CGALPASLLEAELFGHRRGAFTGAVGERTGLVRSAEGGTLFLDEIGELPPASQTAFLR
                     VLQEREVVPIGDDRPVKVDVRLCAATHRDLAGLVERGLFRDDLFGRISGFTLALPTLA
                     ERRADFGLLLRALLAQIPGASDLRFAPPALRALLTHRWPLNIRALEKTLLTAATLATE
                     GVIQPAHLVELVRRPLLADESRASGTPPPTFIAPRSQHDEALRERLVGLLTVHHGNVV
                     AVSRAIGTRRTQIYRWARRFGIDLDGFRR"
     gene            complement(3050..>3498)
                     /locus_tag="VF516_21210"
     CDS             complement(3050..>3498)
                     /locus_tag="VF516_21210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110270.1"
                     /translation="RSRFAPEPDAPSLRPSRRWKASLVAGAVTAAAGGAYAWYSFERQ
                     QAWVPRVHAEVGPGDCGESDTQLLMMPSISLDAFHRTCHFHTHIYYGYAIAGVGAAAA
                     VVSLIMLTRDPVASDSRVAGARARPGLAIAPLIAPDLAGAQIAVAW"
BASE COUNT          421 a         1291 c         1293 g          493 t
ORIGIN      
        1 ctgcggcgcc gccgcgacct cgggcggctt ggccttgagc ttgcgcgccg cggcgaccgt
       61 gtgggggccg atcttgccgt cggccgccag accgtggttg ctctgccagc gcgcgaccgc
      121 ctgcggatcg accttgcccg cgccgtcgag ccggcacacg tcgctggtca gctcgttgaa
      181 ctcgtcgacc agctcggcgt gggcggcgtt gtacgcccgc gcgccggcga cccaggctgg
      241 ctcggcagcg gcctgcgcgg ccctgggcgc ggccgccggc ccggtcttct gcggcccatc
      301 cgactcgtcg tggagcgccg cgatcatctc gctgcccgac agcggatgcg aatccccggt
      361 cgcctggtgg acctcgccct ggctgagcgc gccgctgcga ccctgcgcct tccagctctt
      421 cgcgagctgg atcgcgcgct gaacggcggc gttccccatg tgcgacgccg cgaccgcgag
      481 gatcgccccc aggaagctcg ggtagttctg gatcatctcg gccagcgcgc cggcgtcggc
      541 cggggtcatc cggaggatct gtgcgtgcag atcggcgcgg aggccatccc tcgaatccgc
      601 ggtctcggtc gccggcgccg ccgcgtcctg ctgctgcccg ggttgatgct cgatctgctg
      661 tgcgttgctc atggtccttg gccccctcaa catacgccgc gtgcgccgcg atgctgcccg
      721 cggccggccg ggaaactgcg gcagcctacg ggtttcgcgg tgttgccggc gggacgacct
      781 ccatcgtgag gttgcacgac acgcagatcc gcggccgctc gccgcggtag gcgtggacgt
      841 agtgctgcag ccacccgggg aagatcacca gcgccccggt gcgcggcgcg aggtcgaagg
      901 tctcgccgaa caggctcagg tcggggatgc tgcggccgct gcgccgcggg tcgaggaacg
      961 cgatccggcc cgacggcgcc ggcaggtcgt cgccggcgtc gacgtagtac gcgaccgacc
     1021 agtgggcgtc gccgtgatcg tgggtgatca cgtagtggcc gctgcgcatg atcatcgccc
     1081 acgcggtcac cgcgtagcgg tacgccggga gcgcgtcgcc tcccgcgtgc gcggcgagcg
     1141 cggccacgtg ggcgccgacc gagtccacca ccgcgcgcat cagcgcgcga aaccacggct
     1201 ggggccggcg gctcaggtcc ggcaccgagt gccagccgcc gacgttggcg cgctgccagc
     1261 tcggcacggt ctgctcctcg gcgagcagct gggcggcgat ctcgcggttg acctccgcca
     1321 tgtccggcag atcgaagacg acgatcggcg tcgggaacag cgcgattgcg agcgagggct
     1381 cgaccatcgc cggattatgc acggtcccgg cgccgggtcc gcgcggtgtc tcgaccgtct
     1441 cccggcgcgc ggggtgtgtc accggccgtg tgacacgtcc ggagacacac cggcggtcgc
     1501 gatcggacgg ttcgtatgga gtcccgtgca gttcgacccg tctgcagggt cctggcggcg
     1561 cacggtacga tcgctgcatg gcccggacgc gtgcaggaca ggctcgggca tgacctcggc
     1621 ggcgatacca tcaccagcgt ctcgcacggt cggatcggcg agctcgatcc cggcgtgcag
     1681 ccgtacctgg tcgtactggc ggtcagcgac gacctgcacg cgccgtcgtc gcgccacctg
     1741 ctggacgaga tcgacgaggt gcggttcggc cgcgggccga ggtccgcgac ccggacgacg
     1801 atcggcgaga cccgcgtgct ggagctgcgg atcccggacg cctggatgtc gagccagcac
     1861 ggccggctgg tccgcgggcc cgccggctgg gtgctcgacg acccggggtc caagaacggc
     1921 tcgctgatca acggccaggt cgtgcgccag ggcgtggtca gcgacggcgc gctgatcgag
     1981 ctcggccaca gcttcctcct gttctgcgag cagccggtgg agcacgacgc ggcggacgac
     2041 ctgatcgacg gcgagctggc cgtgctgtcg cccgcgctcg ccacgttctt cggcccgctc
     2101 gcggacggct tcgccgcgct cggccggctg gcccggacgc cggtgtcgat cgtggtgctc
     2161 ggcgagaccg gcaccggcaa ggaggtcgtc gcccgcgcgc tgcacgcgat gtcggggcgg
     2221 accggcgcgt tcgtcgcggt caactgcggc gcgctgccgg cgtcgctgct cgaggccgag
     2281 ctgttcggcc accgccgcgg cgcgttcacc ggcgcggtcg gcgagcgcac cgggctggtc
     2341 cgcagcgcgg agggcggcac gctgttcctc gacgagatcg gcgagctgcc gccggcgtcg
     2401 cagaccgcgt tcctccgggt gctccaggag cgcgaggtcg tgccgatcgg cgacgatcgc
     2461 ccggtcaagg tcgacgtccg gctgtgcgcc gcgacccacc gcgacctcgc cgggctggtc
     2521 gagcgcgggc tgttccgcga cgacctgttc ggccggatct cggggttcac gctcgcgctg
     2581 ccgacgctgg ccgagcgccg cgccgacttc ggcctgctgc tgcgcgcgct gctcgcccag
     2641 atcccgggcg cgagcgacct gcggttcgcg ccgcccgcgc tgcgcgccct gctgacccat
     2701 cgctggccgc tcaacatccg cgcgctggag aagaccctgc tcaccgcggc gacgctcgcg
     2761 accgaaggcg tgatccagcc cgcgcacctg gtcgagctgg tgcggcgccc gctgctcgcc
     2821 gacgagtcac gcgcctcggg gaccccgccg cccacgttca tcgcgccgcg cagccagcac
     2881 gacgaggcgc tgcgcgagcg actggttggc ctgctcaccg tgcaccacgg caatgtcgtc
     2941 gcggtcagcc gcgcgatcgg cacccgccgg acccagatct accgctgggc gcggcggttc
     3001 ggcatcgacc tcgacgggtt ccgtcgctag cgtcgcgagc gtcgcccggt caccaggcga
     3061 ccgcgatctg cgccccggcg agatcgggcg cgatcagcgg ggcgatcgcc aggccgggcc
     3121 tcgcgcgcgc acccgcgacg cggctgtccg acgccaccgg atcgcgggtc agcatgatca
     3181 gcgacaccac cgcggccgcg gcgccgaccc cggcgatcgc gtagccgtag tagatgtggg
     3241 tgtggaagtg gcacgtgcgg tgaaacgcat cgagcgagat gctcggcatc atcaggagct
     3301 gggtatcgct ctcgccgcag tcgcccgggc cgacctcggc gtggacgcgc ggaacccacg
     3361 cctgctggcg ctcgaagctg taccaggcgt acgcgccgcc ggcggccgcg gtcaccgccc
     3421 ccgcgaccag cgacgccttc caccgccgcg acggccggag cgacggggcg tccggctcgg
     3481 gggcgaaccg gctccggg
//
LOCUS       DASVOR010001111         3678 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_449436,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001111 DASVOR010000000
VERSION     DASVOR010001111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3678)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3678)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3678
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449436"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF516_21215"
     CDS             complement(<1..432)
                     /locus_tag="VF516_21215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110271.1"
                     /translation="MLYGRSSLAVAIAVGALGAHPAFAEDAPAPRRLTLAGAIEIALS
                     GNPQLAIDAEAVSAADARARSDAALRLPLLGLKSNVVFWNRAIEADLGPDVGKVTVRD
                     RITGTVDLQVSQPVTGALAIGTLVERDRATAEASRAQRDARR"
     gene            complement(744..1190)
                     /locus_tag="VF516_21220"
     CDS             complement(744..1190)
                     /locus_tag="VF516_21220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110272.1"
                     /translation="MPTSPDLASAAIFSVPEVDWSISGGGTATLHYDLPLGLVGGTIS
                     IQLSGPIPSGATSVQLTGSNGTGTCTASGTVISCGESLTNLGTLPISMTVVQQQAIAT
                     FSGPVSSRVAVANVFSSDPIGTVSFDVTQPSSDGGGGGGGGGPGRH"
     gene            1514..3499
                     /locus_tag="VF516_21225"
     CDS             1514..3499
                     /locus_tag="VF516_21225"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT family oligopeptide transporter"
                     /protein_id="HEX8110273.1"
                     /translation="MMLVQEWNTGSRGGCSTLAGGMADDDVPTADKPEIDPVLDPRAV
                     SQLDEAAWIRRAYRRGAPQLTVRAVATGLLLGFVLSFANVYIGLKTGWFFSMALAACM
                     ASFALWRLLASLGVARGPLSLLEANCMQSTASSAAYATGNMVVGVFPAMLLLSVSPAA
                     PAGVQPHWAALAAWIACVAALGVTLAIPLKRQLINRERLPFPSGMAAAIMLDSLHRTA
                     AGMRSRARTLVAAIAVGAILPVLRDLRGMAVIGQSSKLFDWLPKITAGGVRHPVSDAG
                     LVLDHSLLLVGAGVFVGLRTTVWMVAGGLFTGFVLGPIGLTSSWTDPLGHVVAATSKL
                     GSARIELGIWLGSPLLIAYALVALVDQWRAFVRTFAAWRAPSAPDAASAAIEIPPSWF
                     WCGFTLCGSAAIALGRALFDIPVPLGCLAVATSLVFGMVASRICGETDINPGGPMGKL
                     TQLGFGILRPQHPSTNLMTAAMTHASSVAAADLLNDLKSGYLLGADPRRQFFAQAAGI
                     AAGTAASVLAYFLLIPDAFALTGHGSAPQFAAPAAHLFRAIAELLQYGLDHLHPLHRT
                     LAVAGAIGGVVVAGAERLVPPRVARWLPSAAGLGLGLYMPLSYSLAMGIGAAIAAIAT
                     ARRGDAAERSVWPISAGVLGGESLAGVVVAVINNFTQ"
     gene            complement(3489..>3678)
                     /locus_tag="VF516_21230"
     CDS             complement(3489..>3678)
                     /locus_tag="VF516_21230"
                     /inference="COORDINATES: protein motif:HMM:NF019884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8110274.1"
                     /translation="PRQLIYQRLDEVEQASVPELAKTLELSNSTVLRALRTLIDERRV
                     ESVGFARATRYRLRAPTA"
BASE COUNT          497 a         1334 c         1288 g          559 t
ORIGIN      
        1 ccggcgcgcg tcgcgctgcg cgcggctggc ctcggcggtc gcgcgatccc gctcgaccag
       61 cgtcccgatc gcgagcgcgc cggtcaccgg ctgcgagacc tggaggtcga ccgtgccggt
      121 gatccggtcg cgcacggtga ccttgccgac gtcggggccg aggtcggcct cgatcgcgcg
      181 gttccagaac acgacgttcg acttgagccc gagcagcggc aggcgcagcg ccgcgtcgga
      241 gcgcgcccgg gcatccgccg ccgagaccgc ctcggcgtcg atcgcgagct gcggattgcc
      301 cgacagcgcg atctcgatcg caccggccag cgtcagccgg cgcggcgccg gcgcgtcctc
      361 ggcgaacgcc ggatgcgcgc ccagcgcccc gaccgcgatc gccaccgcca gactcgatcg
      421 tccgtacagc atcaatgcaa acctcccggt gcgttcgccg acccgtgacc cgtttgcatg
      481 cgccgttgtg gccatgcctc gccatccccc atcgcgaacg tcgcgtagtg cacgaggtgt
      541 gcctcggccg cgcagaccgg aaacgcagcg aagtcggcga acacgaacgg cgtctccgcc
      601 cgcgaggagt tcgttcaagc aatgcgacag cgacaccccg cgactgtcgc ggggctgcag
      661 gcgctgtgcg acgcgcgccg ggagatcaac gcgcgcatgg gcagcgcgca accgctgcct
      721 cgccgctcaa gaccggcgaa tgctcagtgc cggcccgggc cgccgccacc gccaccgccg
      781 ccaccatcgc tgctcggctg ggtcacatcg aagctgaccg tgccgatcgg atccgacgag
      841 aacacgttgg cgaccgcgac gcggctggac accggtcccg agaacgtggc gatcgcctgc
      901 tgctggacga cggtcatgct gatcggcagc gtgccgaggt tggtgagcga ctcgccgcac
      961 gagatcaccg tgccgctcgc ggtgcaggtg ccggtgccgt tcgagcccgt gagctgaacc
     1021 gaggtcgcgc ctgaagggat cggcccgctg agctggatcg agatcgtgcc accgaccaga
     1081 ccgagcggta ggtcgtaatg caaggtcgcg gtgccaccgc cggagatcga ccagtcgacc
     1141 tcgggcaccg agaagatcgc cgccgacgcg aggtccggtg aggtcggcac cacgtaggtt
     1201 ccaacgaacc gaccaggggt cgtcaggacg gtttgcgtcg ttacgctgtc tcgatcgact
     1261 gtttccgagg tcgcgggagg atcgattgca cagccggtga gcgacaggac ggcgagtcca
     1321 gagagagaga tccgcatgct gactccgatg ttgcgggcct tgggcgcggc gaggagcagc
     1381 gctcgcgcgc ggacgatata gaagccgcgc ggggcaacgc gagtaacacg atcttactgt
     1441 tccgggaaag aatgatgcga ttcgtcgata gctcagtaat tcaggcttaa taaatgcgtc
     1501 atgacgcagg gtcatgatgc tggtgcagga atggaacacc ggatcgcgcg gcggctgcag
     1561 tacgctcgcc ggcggaatgg ccgacgacga cgtcccgacg gccgacaagc cggagatcga
     1621 tccggtgctc gatccgcgcg cggtctcgca gctcgacgag gcggcctgga tccgccgcgc
     1681 gtatcgccgc ggagcgccgc agctgaccgt gcgcgcggtc gcgaccgggc ttctgctcgg
     1741 cttcgtgctg tcgttcgcca acgtctacat cggcctcaag accggatggt tcttcagcat
     1801 ggcgctcgcg gcctgcatgg cgtcgttcgc gctgtggcgg ctgctcgcgt cgctcggcgt
     1861 cgcgcgaggc ccgctcagcc tgctcgaggc caactgcatg caatcgaccg cgtcgtcggc
     1921 ggcctacgcg accggcaaca tggtggtcgg cgtgttcccg gcgatgctgt tgctgtcggt
     1981 gtcgccggcc gcgcccgcgg gtgtccagcc gcactgggcc gcgctcgcgg cgtggatcgc
     2041 ctgcgtcgcc gcgctcggcg tcaccctcgc gatcccgctc aagcgccagc tgatcaaccg
     2101 cgagcggctg ccgtttccgt cgggcatggc ggcggcgatc atgctcgaca gcctgcaccg
     2161 caccgccgcc ggcatgcggt cgcgggcgcg cacgctggtc gccgcgatcg cggtcggcgc
     2221 gatcctgccg gtgctgcgcg acctgcgcgg catggccgtg atcggccaga gctcgaagct
     2281 gttcgactgg ctgccgaaga tcaccgcggg cggcgtacgc cacccggtga gcgacgccgg
     2341 gctggtgctc gatcacagcc tgctgctggt cggcgccggc gtgttcgtcg gcctgcgcac
     2401 cacggtatgg atggtcgccg ggggcctgtt caccggcttc gtgctcgggc cgatcggcct
     2461 cacgtcgagc tggaccgatc cgctcggcca cgtcgtcgcc gcgaccagca agctcggcag
     2521 cgcccggatc gagctcggga tctggctcgg ctcgccgctg ctgatcgcgt acgcgctggt
     2581 cgcgctcgtc gatcagtggc gcgcgttcgt ccgcacgttc gccgcctggc gcgcgccgtc
     2641 cgctcccgat gcggcgagcg ccgcgatcga gatcccgccg tcatggttct ggtgcgggtt
     2701 caccctgtgc ggcagcgccg cgatcgcgct cggccgcgcg ctgttcgaca tcccggtgcc
     2761 gctcggatgc ctggcggtcg cgaccagcct ggtgttcggc atggtcgcct cgcggatctg
     2821 cggcgagacc gacatcaacc cgggcggtcc gatgggcaag ctgacccagc tcgggttcgg
     2881 catcctgcga ccgcagcatc cgtcgaccaa cctcatgacc gccgcgatga cccacgcgtc
     2941 gtcggtcgcc gccgccgacc tgctcaacga cctcaagagc ggctacctgc tcggcgccga
     3001 cccgcgccgc cagttcttcg cgcaggcagc cgggatcgcg gccgggacgg ctgcctccgt
     3061 gcttgcctac ttcctcctga tccccgatgc gttcgccctg accggccacg gcagcgcgcc
     3121 gcagttcgcc gcccccgccg cccacctgtt ccgcgcgatc gccgagctcc tgcagtacgg
     3181 gctcgaccac ctgcacccgc tgcaccgcac gctggcggtc gccggcgcga tcggcggcgt
     3241 cgtggtcgcc ggcgccgagc ggctggtgcc accccgggtg gcgcgctggc tgccgtccgc
     3301 cgccgggctc ggcctcggcc tgtacatgcc gctgtcgtac tcgctggcga tggggatcgg
     3361 cgccgcgatc gccgcgatcg ccaccgcgcg ccgcggcgac gccgccgagc gctctgtctg
     3421 gccgatctcg gccggcgtgc tcggcggcga gagcctggcc ggcgtggtcg tcgcggtgat
     3481 aaacaacttc acgcagtagg cgcgcgcagc cggtagcgcg tcgcccgcgc gaacccgacg
     3541 ctctcgaccc gccgctcgtc gatcagcgtg cgcagcgcgc gcagcacggt gctgttcgac
     3601 agctcgagcg tcttcgcgag ctcgggcacc gacgcctgct cgacctcgtc gagccgctgg
     3661 tagatcagct ggcgcggc
//
LOCUS       DASVOR010001112         4985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_419768,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001112 DASVOR010000000
VERSION     DASVOR010001112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4985
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419768"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VF516_21235"
     CDS             complement(<1..1371)
                     /locus_tag="VF516_21235"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8110275.1"
                     /translation="MTNAISEHRETTRLGSFEILHRLARGGMAEIYLARTSGPSGFEK
                     LVVLKKILGKYADKPRYVQLFLEEARIAASLDHPGIAQVYDIGMVDGSYFFAMEHLHG
                     QDVRSILHRAWRIGERLPIEHAVQIARHVASALHFAHEKRRGDGTLLGLVHRDVSPSN
                     ILVTYDGATKLLDFGVAKTAASTVKTRTGALKGKISYMSPEQARGAPLDRRSDIFSLG
                     IVLWEMVTTQRLYRADNDLATLQLIIHQPPRPPSQVCPECPPELERIVLRALAQDPDA
                     RYPTAEQLVLELDELAREHKLKQSPQALAATLGELFGPELAAWREAQAAGVPLADHLH
                     ELGDRTAPIGESELIRLRDELGGGAGFADDEDDGPPGDLDDDFDDEPEDVVLAEVDDA
                     GALDDGSDDDLTEHMAPVVDTLSPLAADPILPETIVLEPIGLEPTPVPTTLVTQTLTV
                     PGTPVAL"
     gene            1517..1717
                     /locus_tag="VF516_21240"
     CDS             1517..1717
                     /locus_tag="VF516_21240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110276.1"
                     /translation="MGETLFSSTYQPNPRSALDVLAFVLTPADAFASGPDEIEAVAVI
                     LEAAKTEAIERWQSLRSAARCS"
     gene            complement(1779..2723)
                     /locus_tag="VF516_21245"
     CDS             complement(1779..2723)
                     /locus_tag="VF516_21245"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8110277.1"
                     /translation="MTTGNENRSYVAAHFALELAGVNDVCTVRSIEGGGLKADVMTYQ
                     PGATYERWRQVGKPKFEDIKLQVGTAMSEPFFRWISDFFAGTCVRKNGAIVAGDFYYK
                     ERARREFTEALITEFTFPKLDASDKNTCYMNVTLGVESIDYKKGDDSKTLDQQGNEAA
                     QRAWKACNFQFHLDGFEDTCARVTKVDSFTIKMPVLEYHSGGSRTTSKTPTMIDFPNI
                     SFYLPEDDAQPFHDYMKKRVIKGEVPGRLHGQLITLDNAKATMFTLELFDCDLLNIAP
                     DKSDATSTEVKQVKVEMYVERMSFNYGGAYGADPPEPT"
     gene            2907..3557
                     /locus_tag="VF516_21250"
     CDS             2907..3557
                     /locus_tag="VF516_21250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110278.1"
                     /translation="MSLPKPRWGFWKGMLAGVAVEVPALTAAVWVLAQLGIGNAEVPV
                     MRIVRLTTVFAGAAAVFTAGGVGRLAAYASIDGGRRRAVFVAARAHAVASAGLVLIAA
                     LPHGQLPGRSLAWLAFPAAGAVVGAACGALIGVVCSGQAPVDLSDVWSLARLPSVALR
                     HWLDPRELVRLGAAVRQHTTHLFEGMFDPAPPPPTMSDPPSPAPTAPIPPPPRDPE"
     gene            3554..4456
                     /locus_tag="VF516_21255"
     CDS             3554..4456
                     /locus_tag="VF516_21255"
                     /inference="COORDINATES: protein motif:HMM:NF024790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX8110279.1"
                     /translation="MKPTDRLELRTLAFALLASLLLWNLPFGGVLLYPFKLLATWLHE
                     LSHGLAMLLTGVGFDRVLIYRDTSGLAYGSSEAGRVASAVIAAAGYMGTPLWGAVLLV
                     VSPDARWARRALLVLAALLVGTALTVIDMPDGDRFGPWAIGAIGAACAAAALVMPGRM
                     RLAMAHFIAAQSCVDALLDIRVLLRPSQVVGGKIAGASDAHNMAQATFGTSATWAVWT
                     WAIVWLLWSLAVLYVALRVSGSRALAQVMPAARGRDLRPGVWIEQARVAHSASARPAL
                     RLRSRRAAARRILARRPRWSRGPR"
     gene            complement(4380..>4985)
                     /locus_tag="VF516_21260"
     CDS             complement(4380..>4985)
                     /locus_tag="VF516_21260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110280.1"
                     /translation="AAAGRVLAIAFAAAAVVAGILVVQTSCLDSLSRGGFSQIHVGDL
                     AIRVPGSWQQPTSPAQPDGPAGQPDRAEVQVYELDGLVVLELARGGSAPKGEIALWIA
                     DRGRRVKDLLRDDDAPGDDAGELIDAGVTPLIALPAGWEGRELVFVGPDDGMGNRQRL
                     RLVACGRASADGTVLAAILVPETIAAAAPGFFAQLLASIEA"
BASE COUNT          634 a         1714 c         1831 g          806 t
ORIGIN      
        1 cagcgcgacc ggcgtgcccg gcacggtgag cgtctgggtg accagcgtgg tgggcaccgg
       61 cgtcggctcc aggccgatgg gctccagaac gatcgtctcg gggaggatcg gatcggccgc
      121 cagcggcgac agcgtgtcga ccaccggcgc catgtgctcg gtcagatcgt cgtcgctgcc
      181 atcgtcgagc gcgccggcgt cgtcgacctc cgcgaggacg acgtcctcgg gctcgtcatc
      241 gaagtcgtcg tcgagatcgc ccggcggccc gtcgtcctcg tcgtcggcga acccggcgcc
      301 gccgccgagc tcgtcgcgca gccggatcag ctcgctctcg ccgatcggcg cggtccggtc
      361 gccgagctcg tggaggtggt cggcgagcgg tacgccggcg gcctgggcct cgcgccacgc
      421 ggcgagctcg gggccgaaca gctcgccgag cgtcgccgcc agcgcctggg gcgactgctt
      481 gagcttgtgc tcgcgggcca gctcgtcgag ctcgagcacg agctgctcgg cggtcgggta
      541 gcgcgcgtcg gggtcctggg ccagcgcgcg cagcacgatg cgctccagct cgggcgggca
      601 ctcggggcag acctgcgacg gcggccgcgg cggctggtgg atgatcagct gcagcgtcgc
      661 gaggtcgttg tcggcgcggt acagccgctg cgtggtgacc atctcccaca gcacgatgcc
      721 gagcgagaag atgtcgctgc ggcggtcgag cggcgcgccg cgcgcctgct ccggggacat
      781 gtacgagatc ttgcccttga gcgccccggt ccgggtcttg accgtgctcg ccgcggtctt
      841 ggcgaccccg aagtcgagca gcttggtcgc gccgtcgtag gtcaccagga tgttcgacgg
      901 cgacacgtcg cggtggacca ggccgagcag cgtgccgtcg ccgcgccgct tctcgtgcgc
      961 gaagtgcagc gccgacgcga cgtggcgcgc gatctggacc gcgtgctcga tcggcagccg
     1021 ctcgccgatc cgccacgcgc gatgcaggat cgaccgcacg tcctggccgt gcaggtgctc
     1081 catcgcgaag aagtagctgc cgtcgaccat cccgatgtcg tagacctgcg cgatgccggg
     1141 gtgatccagg ctggcggcga tcctggcctc ctcgaggaac agctggacgt agcgcggctt
     1201 gtcggcgtac ttgccgagga tcttcttgag cacgaccagc ttctcgaagc ccgacgggcc
     1261 cgaggtgcgc gcgaggtaga tctcggccat cccgccacgc gccagccggt gcaggatctc
     1321 gaacgacccg agccgggtcg tctccctgtg ctccgagatc gcgttggtca cgcccgggcg
     1381 actatatcaa cggccccccg gcggcaagtg gctttcgccg caatctattc gcggacatgc
     1441 gttatgcgcc atccgcgcac acccctgcgc accgccgaaa acttgcggtg cagatccccg
     1501 ggtgtgaggg tgcgccatgg gcgagacgct gttcagcagc acgtaccagc caaacccgcg
     1561 cagtgcgctc gacgtgctcg cgttcgtgct gacgccggcg gacgcgttcg cgagcggccc
     1621 cgacgagatc gaggcggtgg ccgtgatcct ggaggcggcc aagaccgagg cgatcgagcg
     1681 ctggcagtcg ctgcgcagcg cggcgcgctg cagctgaccg cgcgggggcc gaccggggcc
     1741 gcgacggtcg cagccggcgg cgtggtgcat cgtccgggct aggtcggctc gggcgggtct
     1801 gccccgtacg cgcccccgta gttgaacgac atgcgctcga cgtacatctc gaccttgacc
     1861 tgcttgacct ccgtgctcgt cgcatccgac ttgtcgggcg cgatgttcag gagatcgcag
     1921 tcgaacagct ccagcgtgaa catcgtcgcc ttggcgttgt cgagcgtgat gagctggccg
     1981 tgcagccggc ccgggacctc gcccttgatg acgcgcttct tcatgtagtc gtggaacggc
     2041 tgcgcgtcgt cctcgggcag gtagaacgag atgttcggga agtcgatcat cgtcggcgtc
     2101 ttgctcgtcg tgcggctgcc gccggagtgg tactcgagca ccggcatctt gatcgtgaac
     2161 gagtcgacct tggtcacccg cgcgcacgtg tcctcgaagc cgtcgaggtg aaactggaag
     2221 ttgcacgctt tccaggctcg ctgggccgcc tcgttgccct gctggtcgag ggtcttgctg
     2281 tcgtcgccct tcttgtaatc gatgctctcg acgccgagcg tgacgttcat gtagcaggtg
     2341 ttcttgtcgc tggcgtcgag cttggggaac gtgaactcgg tgatcagcgc ctcggtgaac
     2401 tcgcgccgcg cgcgctcctt gtagtagaag tcgccggcga cgatcgcgcc gttcttgcgg
     2461 acgcaggtgc ctgcgaagaa gtccgagatc caccggaaga acggctcgga catcgccgtg
     2521 ccgacctgga gcttgatgtc ctcgaacttg ggcttgccga cctggcgcca gcgctcgtag
     2581 gtggcgcccg gctggtaggt catgacatcg gccttcaacc cgccgccctc gatcgagcgc
     2641 accgtgcaga cgtcgttgac gccggcgagc tccagcgcga agtgcgctgc gacataggag
     2701 cgattctcgt tgcctgtggt catccgtcca gcctcgggcg atagtacacg gggggcgcac
     2761 ccgccaacca ccggcgggcg tgtcgtcgga acatgctacg ctcggaggcc gcgagccctt
     2821 cctagccaca atcctttcgt gggcttcgcg ggccggccat ggacgagcgt cagccagtga
     2881 cgataagtgc ccgatcttct ggagggatgt ccctgcccaa gccgcgctgg gggttctgga
     2941 aggggatgct ggcgggagtt gcggtcgagg tgccggcgct gaccgccgcg gtgtgggtgc
     3001 tcgcacagct cgggatcggc aacgccgagg tacccgtgat gcggatcgta cggctcacca
     3061 cggtgttcgc cggggcagca gccgtcttca ccgcgggggg agtcggccga ctggccgcgt
     3121 atgcatcgat cgacggcgga cgcaggcgcg cggtgttcgt tgcggcgcgg gcgcacgccg
     3181 tggcaagcgc ggggctcgtg ctgatcgcag cgcttcctca cgggcagctg ccgggccgct
     3241 cgctggcctg gctggcgttc ccggccgccg gtgcggtggt cggcgcggcg tgcggtgcgc
     3301 tgatcggtgt ggtgtgcagc gggcaggcgc cggtcgatct ctccgacgtg tggtcgctcg
     3361 cgcgactgcc gagtgtggcg ctgcgccact ggctcgatcc gcgcgagctg gtccggctcg
     3421 gtgccgcagt tcgccagcac acgacgcacc tgttcgaagg catgtttgat cctgccccgc
     3481 cgcccccgac gatgtccgat ccgccgtccc ccgcacccac ggcgcccatc ccccctccgc
     3541 cccgggatcc cgagtgaagc cgaccgaccg cctggagctc cgcacgctgg ccttcgcgct
     3601 gctggcgtcg ctgctgttgt ggaacctgcc gttcggcggc gtgctgctct acccgttcaa
     3661 gctgctcgcg acctggctcc acgagctgtc gcacggcctg gcgatgctgc tgaccggggt
     3721 cgggttcgat cgcgtgctga tctaccgcga caccagcggc ctggcctacg gcagcagcga
     3781 ggccgggcgg gtcgcgagcg cggtgatcgc ggcggccggc tacatgggga cgccgctgtg
     3841 gggcgcggtg ctcctggtgg tgtcccccga cgcgcgctgg gcgcgccggg cgctgctggt
     3901 gctcgccgcg ctgctggtcg gcacggcgct gaccgtgatc gacatgcccg acggcgaccg
     3961 cttcgggccg tgggcgatcg gcgcgatcgg cgcggcgtgc gccgccgcgg cgctggtgat
     4021 gccgggccgg atgcggctgg cgatggcgca cttcatcgcg gcgcagtcgt gcgtcgacgc
     4081 gctgctcgac atccgcgtgc tgctccggcc gtcgcaggtg gtcgggggca agatcgccgg
     4141 cgcgtcggat gcccacaaca tggcgcaggc gacgttcggc acgtccgcga cctgggcggt
     4201 gtggacgtgg gcgatcgtgt ggctgttgtg gtcgctcgcg gtgctctacg tcgcgctgcg
     4261 ggtcagcggc tcgcgcgcgc tcgcgcaggt catgccggcg gcgcgcggcc gcgacctgcg
     4321 gcctggggtc tggatcgagc aggcccgggt cgcgcactcc gcgagcgccc ggccggcgct
     4381 caggcttcga tcgaggcgag cagctgcgcg aagaatcctg gcgcggcggc cgcgatggtc
     4441 tcggggacca agatagccgc gagcacggtg ccgtcggcgg acgcgcggcc gcacgcgacg
     4501 agccgcaggc gctggcggtt gcccatgcca tcgtcgggtc caacgaagac gagctcccgg
     4561 ccctcccagc cggcgggcag cgcgatcagc ggggtgacgc cggcgtcgat cagctcgccg
     4621 gcgtcatcgc cgggcgcatc gtcatcgcgg agcaggtcct tgacgcgccg gccccggtcg
     4681 gcgatccaca gcgcgatctc ccctttcggg gcgcttccgc cgcgcgcgag ctcgagcacc
     4741 accagcccgt cgagctcgta cacctgaacc tcggcgcggt cgggctgccc cgccgggccg
     4801 tcgggctgcg ccgggctggt cggctgctgc cagctgcccg gcacccggat cgcgaggtca
     4861 ccgacatgga tctggctgaa cccaccccgg gacaggctgt ccaggcacga ggtctgcacg
     4921 acgaggatcc cggcgacgac cgccgcggcg gcgaacgcga tcgcgagcac gcggccggcg
     4981 gcagc
//
LOCUS       DASVOR010001113         2203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_157227,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001113 DASVOR010000000
VERSION     DASVOR010001113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2203
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157227"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VF516_21265"
     CDS             complement(<1..275)
                     /locus_tag="VF516_21265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110281.1"
                     /translation="MSDHPPRWRTCSTSSSGGDLVEALLGGRGAVGARELGFGITAAL
                     AIVGFVWRARGRRVLALLSPEIAATAGIRVARPRCSRRAMTQPRDVGS"
     gene            775..>2203
                     /locus_tag="VF516_21270"
     CDS             775..>2203
                     /locus_tag="VF516_21270"
                     /inference="COORDINATES: protein motif:HMM:NF024996.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX8110282.1"
                     /translation="MVAGQPASEEPIPLVEIYGTLVPFLEYGHTTGATAPGQYMPNGA
                     TGAAMNGPNLVPTTGYTGINQSSRGVMDPSTSNIGFRGGIELMPNLSVVWQVESAIPV
                     EGTGPANTWASRNSNIGITGNWGTLFFGSWDTPYAWTTRTLVNPIKSGNLSDYNSIIN
                     NPGFGVSSVTTQSTRANTSADAAFERRQGNAIQYWTPTISGVSARFAYSVNEGRTAAP
                     APAPSPSPIVASGAIGFETGPITLREGVEVHYDYFGMSFQGGSMPATATNRSSTDWGS
                     KTVAQFLNKAPGFDTRVIGVAEYLSYKNKDNAAPNMMNPALNANTEHARWSFYGLIDQ
                     TLAGKHHIWVGGGKAMEGTCAKVGGAACSTTGLSSIDTVVGYIYRASKSTDFWAAAYR
                     ISNDFAASYTTSPSLGAPPAPGASIEAFGVGILYTFSAKIIGPPPKTPVPPAPVPAAT
                     PTPAPTNEPGPVPPPSAPEPPPTPGT"
BASE COUNT          370 a          795 c          709 g          329 t
ORIGIN      
        1 gatccgacat ctcgcggttg cgtcatggct cgccgggaac agcgcggccg ggcgacccgg
       61 atcccggcgg tcgcggcgat ctccggcgac agcagggcaa gcacccgccg gccgcgggcg
      121 cgccacacga agccgacgat cgccagcgcg gcggtgatgc cgaacccgag ctcgcgggcg
      181 ccgaccgcgc cgcgcccgcc gagcagcgcc tcgaccaggt ccccgcccga gctcgacgtc
      241 gagcacgttc gccatcgcgg aggatgatcc gacatctcgc ggttgcgtca tggctcgccg
      301 ggcgcccaaa tgccgggcca ttccgtgccc ggcggccttg cggccgagcg tcgatccatg
      361 atcaagttcg aacggtgtgt tgcgggggca accgcctcgg atcccgatcg gccatgtcag
      421 ctgtgcgcga caatcgtcag tgcgttgtca gattcgaccc aaaacactgc attttccata
      481 aattagaatc gtcgagtgac gagcagttta tgcgtgtccg atacacccgg cgttccgcgc
      541 tgctcagaaa tttgaacacg gcatcgcgtg ctctgtaacg attgcaactc gtgatctgcg
      601 cgcgtatggt cggtgctgcc atcgagctga caccggccgg tgtccgtcca gtccctggcc
      661 tcgacgtggg gcccgggtcg atgtgggtgc ccttcactgc gaggtgatcc aatgaagtcg
      721 ccagtctcac tacctgtcgc actggctctt gtgggagcga gcctcttccc gcgtgtggtc
      781 gccggtcagc cggcgtccga ggagccgatt ccgctggtcg agatctacgg aacgctcgtt
      841 ccgttcctgg agtacgggca caccacgggc gccaccgcgc cgggccagta catgccgaac
      901 ggcgcgaccg gcgccgccat gaatggaccg aacctggtcc cgaccaccgg gtataccggc
      961 atcaaccagt cgtcgcgcgg cgtcatggat ccgagcacca gcaacatcgg attccgcggc
     1021 ggcatcgagc tgatgccgaa cctgtcggtg gtctggcagg tcgagagtgc gatcccggtc
     1081 gaagggaccg gtccggccaa cacgtgggcg agccgcaaca gcaatattgg aatcaccggg
     1141 aactggggaa cgctgttctt cggcagctgg gatacgccgt acgcgtggac cacgcgcacg
     1201 ctggtcaatc cgatcaagtc cggcaacctg tccgactaca acagcatcat caacaacccg
     1261 gggttcggcg tcagctcggt gaccacccag tcgacccgcg ccaacacctc ggccgacgcc
     1321 gcgttcgagc gccggcaggg caacgccatc cagtactgga cgccgaccat ctcgggggtc
     1381 tcggcgcggt tcgcctactc ggtcaacgag ggccgcacgg cggctcccgc gccggcgccg
     1441 tcgccctcgc cgatcgtggc cagcggcgcg atcggcttcg agaccggccc gatcacgctg
     1501 cgcgagggcg tcgaggtcca ctacgactac ttcggcatgt cgttccaggg cggctcgatg
     1561 ccggcgaccg ccaccaaccg gtcgtccacc gactggggca gcaagacggt ggcgcagttc
     1621 ctcaacaagg cgcccggctt cgacacccgc gtgatcggcg tcgccgagta cctgtcgtac
     1681 aagaacaagg acaacgcggc gccgaacatg atgaacccgg cgctgaacgc caacaccgag
     1741 cacgcgcggt ggtcgttcta cggcctgatc gaccagaccc tggccggcaa gcaccacatc
     1801 tgggtcggcg gcggcaaggc gatggagggc acgtgcgcca aggtcggcgg cgcggcctgc
     1861 agcaccaccg gcctgagctc gatcgacacc gtggttggct acatctaccg ggcctccaag
     1921 tcgacggact tctgggccgc ggcgtaccgg atcagcaacg acttcgcggc gagctacacc
     1981 acctctccgt cgctcggcgc cccgcccgcc ccgggcgcct cgatcgaggc gttcggcgtc
     2041 ggcatcctct acacgttctc ggccaagatc atcggaccgc cgcccaagac accggttccc
     2101 ccggcgcctg ttccggccgc cacgccgacc ccggcgccga ccaacgagcc cggcccggtg
     2161 ccgccgccga gcgcgcccga gccgccgccc accccgggca cgc
//
LOCUS       DASVOR010001114         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_186935,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001114 DASVOR010000000
VERSION     DASVOR010001114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186935"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /locus_tag="VF516_21275"
     CDS             <1..1176
                     /locus_tag="VF516_21275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110283.1"
                     /translation="GKTTLALNVIEQALEHDIAVILLDRKGDLAGYAHPDWWQHTADP
                     RRARQLAERIDVRLFTPGTRGGRPLALPVIPDLASIPDHERDRMVQHAANAIAAMMRF
                     GTGAADTARLAILTQAIAVLAGRPRTSGLGELITLVEARDDELVARAGRYDDRLFKRL
                     VQDLETVRLSDADLFDASAEPLTAETLIGRGADGKVPLAIASTRFLGDGPRIQSWVAH
                     LIGCLSRHAARTPSSVLRTLLVIDEADLFMPAGTAKPASKEPLQDLLKRARTAGLGLV
                     LASQSPADFDYRSRELINTWFLGRIADQRSIDKMKPLFEHKPFIRGKLGLLEAGRFVM
                     LQDGEAFDLERGPSLMRTEQLPEAELMALAARTHPRPPRAARPPLRSDALRSDAPPG"
     gene            1337..1684
                     /locus_tag="VF516_21280"
     CDS             1337..1684
                     /locus_tag="VF516_21280"
                     /inference="COORDINATES: protein motif:HMM:NF018767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensory rhodopsin transducer"
                     /protein_id="HEX8110284.1"
                     /translation="MTSAAIGRTRWAIAEGYIPAWSHGPPHLFTSHETACLLNTSDTD
                     AHVRITLYFSDREPVGPYRVTVPARRTRHLRFNDLHDPQPVPVATDYASVIESDVPIS
                     ENALLSTVAYPGG"
     gene            complement(1701..>2433)
                     /locus_tag="VF516_21285"
     CDS             complement(1701..>2433)
                     /locus_tag="VF516_21285"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX8110285.1"
                     /translation="RAAALDDQAEQLRIRFERAFWSESIGSYALALDGDKRPCEVRAS
                     NAGHALFTRIASADRARRVVDALLDEHAFSGWGIRTLDARDARYNPISYHNGSVWPHD
                     NALIAAGMARYGHKQACARVTAALHQAALHFDLRRMPELFCGFRSRPLEGPTLYPVAC
                     APQAWAAGAVFLLLQACLGLEVDALRAQVVLDRPILPPSLDQLTIRRLAVGDARVDLV
                     IERHPHDAGVYLLSRDGDVSVVVTK"
BASE COUNT          366 a          874 c          879 g          314 t
ORIGIN      
        1 ggcaagacca cgctggcgct caacgtcatc gagcaggcgc tggagcacga tatcgccgtg
       61 atcctgctcg atcgcaaagg agatctcgcc ggctacgcgc atccggactg gtggcagcac
      121 accgcggatc cccggcgcgc ccgccagctg gcggagcgca tcgacgtccg cctgttcacc
      181 ccgggcacgc gcggcggccg gccgctggcg ctgccggtga tcccggatct cgcgtcgatc
      241 ccggaccacg agcgcgatcg gatggtgcag cacgccgcga acgcgatcgc cgcgatgatg
      301 cgattcggca ccggcgcggc ggacaccgcc cggctcgcca tcctcaccca ggcgatcgcg
      361 gtgctcgccg ggcgtcccag gacgagcggc ctcggtgagc tgatcacgct ggtcgaggcc
      421 cgtgacgacg agctggtcgc acgcgccggc cgctacgacg accggctgtt caagcgcctg
      481 gtgcaagacc tggagaccgt gcggctgagc gacgcggacc tgttcgatgc gagcgccgaa
      541 ccattgacgg ccgagaccct gatcggccgg ggtgcggacg gcaaggtccc gctcgcgatc
      601 gcgagcacgc ggttcctcgg agacgggcct cggatccagt cgtgggtggc acacctgatc
      661 ggatgcctga gccgccacgc cgcccggacg ccgagcagcg tactccgcac gctgctcgtc
      721 atcgacgaag ccgatctgtt catgccggcg ggcaccgcca agcccgcgtc caaggagccg
      781 ctgcaggatc tgctcaagcg ggcacggacc gcaggcctcg gcctggtgct cgcgtcccag
      841 agcccggcgg acttcgacta ccggagccgc gagctgatca acacctggtt cctcggccgg
      901 atcgccgacc agcggtcgat cgacaagatg aagccgctgt tcgagcacaa gccattcatt
      961 cgcggcaagc tcggtttgct ggaggccggc cggttcgtga tgctgcagga cggcgaggcc
     1021 ttcgacctgg agcgcggtcc gtcgctgatg cgtaccgagc agctgcccga ggccgagctc
     1081 atggcacttg ccgcgcggac ccacccgcga ccgccgcgag cggcgcggcc gccgctgcgc
     1141 agcgatgcgc tgcgttccga cgcaccgccc ggatagcatc aggggcgtgc tcgggcgcac
     1201 ggctgcacgg tcaccggtac ggagcgcccg gcgcggccgg cggatcgacg ccccggtgcg
     1261 tctgcgacct ggagcgagac tggcaggtcg tccggtcagg aggtcccatg cgcatccgag
     1321 atccagcgag gtggccatga ccagcgccgc gatcggccgt acgcggtggg cgatcgccga
     1381 gggctacatc ccggcgtgga gccacggccc gccgcacctg ttcaccagcc acgagaccgc
     1441 gtgcctgctc aacacgtcgg acaccgatgc acacgtccgc atcacgctgt acttcagcga
     1501 tcgcgagccg gtcggtccgt accgcgtgac ggtgccggcc cggcggaccc ggcacctccg
     1561 gttcaacgac ctgcacgatc cgcagccggt gccggtcgcg accgactacg cgagcgtgat
     1621 cgagtccgac gtaccgatct cggagaacgc gctgctcagc acggtcgcgt acccgggcgg
     1681 ctagagcggc ggcgaccgcg ctacttggtg acgaccacgc tgacgtcgcc gtcgcgcgac
     1741 agcaggtaga cgccggcgtc gtgcgggtgg cgctcgatca cgaggtcgac ccgggcgtcg
     1801 ccgaccgcga gccggcggat cgtgagctgg tcgaggctcg gcggcaggat cggccggtcg
     1861 agcacgacct gcgcgcgcag cgcgtcgacc tcgagcccga ggcaggcctg gagcaggagg
     1921 aacaccgcgc cggcggccca cgcctgcggc gcgcacgcga ccgggtacag cgtcgggccc
     1981 tcgagcggcc gcgaccggaa cccgcagaac agctccggca tgcgccgcag atcgaagtgc
     2041 agcgcggcct ggtggagcgc cgcggtgacc cgcgcgcagg cctgcttgtg accgtagcgc
     2101 gccatgccgg cggcgatcag cgcgttgtcg tgcggccaca ccgagccgtt gtggtaggag
     2161 atcgggttgt agcgcgcgtc gcgggcgtcg agcgtgcgga tcccccagcc ggagaacgcg
     2221 tgctcgtcga gcagcgcgtc gaccacccgg cgcgcccggt cggcgctcgc gatccgggtg
     2281 aacagggcgt ggccggcgtt ggacgcgcgg acctcgcacg gccgcttgtc gccgtcgagc
     2341 gcgagcgcgt agctaccgat cgactcggac cagaacgcgc gctcgaaccg gatccgcagc
     2401 tgctcggcct gatcgtcgag cgcggcggcg cgg
//
LOCUS       DASVOR010001115         1989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_273648,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001115 DASVOR010000000
VERSION     DASVOR010001115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273648"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF516_21290"
     CDS             complement(<1..262)
                     /locus_tag="VF516_21290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110286.1"
                     /translation="MKTVRMPVRPRRAWRTVTCHDSESRRQLTPPGGYVGSPPYMALE
                     LWVGADAAGPEADIYALAIVAYETLTGRRLFVANRTSEYFEQH"
     gene            884..1759
                     /locus_tag="VF516_21295"
     CDS             884..1759
                     /locus_tag="VF516_21295"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX8110287.1"
                     /translation="MEVDLHQLELRHRDLRIHDGEQRRRLIGSVAEIGQQVPVVVIHD
                     ADRLVLIDGYLRVEALRRLHRDTAIATAWPVSEVEALVHHRHLAGAKRTALEDAWLLG
                     RLHEHGLTMEQLARQLCRSTSWVSRRLGLLDALDGTAQDLVRAGTVPAHAAMKYLVPL
                     ARANKAQCTQLIAGLGDTRVSDRELGALYAAWRRADGAGRRRIVSEPLLVLRALAAMT
                     EAAEDETVSLHKDLTVLAAVALRAGRRAREGGRFTARLTQAWRAASEAFAALGATIEE
                     IHAGAEPTTGHPDLA"
     gene            1716..>1989
                     /locus_tag="VF516_21300"
     CDS             1716..>1989
                     /locus_tag="VF516_21300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110288.1"
                     /translation="MLEQSQRQAILTLRKAGHGIRAIARTLGISRIAVKSVLASGDPR
                     PPPLERTEKADAHRDDILALYAECKGNLVRVHEELATRGIVALSYPA"
BASE COUNT          312 a          630 c          768 g          279 t
ORIGIN      
        1 tgtgctgttc gaagtactcg gacgtccggt tcgcgacgaa cagccggcgc ccggtcagcg
       61 tctcgtaagc aacgatggcg agcgcgtaga tgtcggcctc cggcccggcg gcgtcggcac
      121 cgacccacag ctccagagcc atgtacggag gcgagccgac gtacccaccc ggcggcgtga
      181 gctggcgcct gctctccgag tcatggcagg tgacggtgcg ccacgcgcgg cgcggccgga
      241 ccggcatgcg cacggtcttc acatcatcgt ggtcggaatc ccttcgacgc tcggcagaaa
      301 tgacccgggg ttgctcgtca gaaatgaccc accgacctga tcgacagcga gagctcgtgg
      361 ccggcgatgg gtgtgggtgg gtggtgtcgg ggccggcggg catcgtggcg ttgggcgatg
      421 gtcgtgggcg ctgtgcgcaa gaagcgtccg tgttgcatat gccggcgatg gtttctgcct
      481 gacgctcggg tcggcgatcg gcagcgggcc tgcggcgccg caagctgtca gcgggcgcgc
      541 cacgagcgag cgtaccgaga gtggcgggac gcgaatcgcg actacgatcg aggacgccgt
      601 tggcaagaca cacttggcgc ggccaaggcg gagcccgcgt cggcaccgcc ggcggccaat
      661 gcgccgcccc cagtagcggg agtgccctgg gatgtcgtgc aaaacgagat gcgggtggaa
      721 ggtcgcgtga tccttgcggg agtcgtccga gtaatcgggg tgttcgtgca aaacgagatg
      781 cgaagacaag tcatggaaat gacaccggga gtagcgagac atgcccgggt agcgccgcaa
      841 accgagatag ggggattccc gtagttccgg cagcctgccc agcgtggagg tggacctgca
      901 ccagctcgag ctccggcacc gcgatctgcg gatccacgac ggcgagcagc gccggcggct
      961 gatcggatcg gtggccgaga tcgggcagca ggtgccggtg gtggtgatcc acgacgccga
     1021 tcgactggtg ctgatcgacg gctacctccg cgtcgaagcg ctgcgccggc tgcaccgcga
     1081 cacggcgatc gcgacggcgt ggccggtctc ggaggtcgag gcgctggtgc atcatcgcca
     1141 cctggcgggg gccaagcgga ccgcgctcga ggacgcgtgg ttgctgggcc ggctgcacga
     1201 gcacggcttg acgatggagc agctggctcg ccagctgtgc cgctcgacga gctgggtgtc
     1261 gcggcggctg gggttgctcg acgccctcga cggcacggcg caggacctgg tgcgcgcggg
     1321 gaccgtgccg gcgcacgcgg cgatgaaata cctggtgccg ttggcgcgcg ccaacaaggc
     1381 gcaatgcaca cagctgatcg ccgggctggg cgacacgcgc gtgtccgatc gcgagctcgg
     1441 cgcgctgtac gcggcctgga ggcgcgcgga tggggccggc cggcggcgga tcgtgagcga
     1501 gccgctgctg gtgttgcggg cgctggcggc gatgaccgag gcagccgaag acgagaccgt
     1561 gtcgctgcac aaagatctga ccgtgcttgc cgccgtggcg ctgcgcgcgg ggcggcgggc
     1621 gcgcgagggc ggtcggttca ccgcccgatt gacccaggcg tggcgcgccg cgagcgaggc
     1681 gttcgcggcg ctcggcgcca ccatcgagga gatccatgct ggagcagagc caacgacagg
     1741 ccatcctgac cttgcgtaaa gcgggccacg gcatccgcgc catcgcccgc acgctgggca
     1801 tctcgcgcat cgccgtgaag agcgtgctcg ccagcggcga cccgcggcca ccgccgctgg
     1861 agcggaccga gaaggccgac gctcaccgcg acgacatcct ggcgctctac gcggagtgca
     1921 agggcaatct ggtgcgggtg cacgaggagc tggcgacgcg cggcatcgtg gcgctgtcgt
     1981 acccggcgc
//
LOCUS       DASVOR010001116         8856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_419822,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001116 DASVOR010000000
VERSION     DASVOR010001116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419822"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="VF516_21305"
     CDS             <1..655
                     /locus_tag="VF516_21305"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8110289.1"
                     /translation="VGPSGCGKSTLLKILAGLLRPTEGTAELRGEPITGPRRDIGVVF
                     QTPVLFPWRTVLGNAMLPADVQRLDRRAMQQRALELLGMVGLGGFERRYPRELSGGMQ
                     QRVGIVRALLHDPAILLMDEPFAALDAMTREAMSVELQRIWLQRRITVLFITHSIAEA
                     VFLADRVIVLTPRPGTIGDQFEVDLPRPRALEVMNTERFGQHVRRVRDALGGRGTVE"
     gene            652..1452
                     /locus_tag="VF516_21310"
     CDS             652..1452
                     /locus_tag="VF516_21310"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8110290.1"
                     /translation="MTRGPLAFVLRLIFLAALIGLWELVVVELDVPAFIVPAPSQVAV
                     ALYRGIESDIYTTHLYVTVHETLFGFALGCALAFVSGSVIALSRTVEYYLYPLIVMFQ
                     SVPKVALVPLMMTWFGVGVTSKVVSAALVAFFPLMVNTVVGLRSADEDRVNLLRSLAA
                     SRWQIFRMLQLPSALPFLFAGLEVALIFALIGAIVAELIGAEKGLGMLTQSMSFSMDT
                     AGQFSILFILAILGLVLNGLLRLVRREVLFWDRSHDAGVLSPTKGDDP"
     gene            1452..2513
                     /locus_tag="VF516_21315"
     CDS             1452..2513
                     /locus_tag="VF516_21315"
                     /inference="COORDINATES: protein motif:HMM:NF024772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8110291.1"
                     /translation="MKRIAIACVLVLGALAAPADADVRTVRVGWCAKTVSSAAAPFAI
                     AQKLGWFEKAGIKVELVPLPGSTDCVKLMATRDVQVSLASMEPLAIVRPQGVKAKFFY
                     TAYQGNIYGIAVPADSPVKSMADLKGKRIGVISMASAGVVVARALAKQAGLDPDKDIS
                     IVVAGESAQTAALLRSKQVDALSQFDTQYALTSNAGAKLRLLDHPEIRKFPSNGFVAL
                     EDYLKTNRADAVALARGYAMGTVFAIANPEAAIRILWEVYPQTRALGKDEATALKDDL
                     LTLDARAKHWRYEPVDGKRWGDNVEANYRAYLDWLIAQGVVKTRVDVKDLITSELLDD
                     VNKFDAEAIRRDARAFKAK"
     gene            complement(2657..3076)
                     /locus_tag="VF516_21320"
     CDS             complement(2657..3076)
                     /locus_tag="VF516_21320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110292.1"
                     /translation="MTEFNGIDIVVQHRSPDASVRRALAAALAVSEERVVVIDDLSNY
                     PEAGAADIVCVSSALKGEFTGLLSIQVERLAVPCETHEQLMRRLCDALGTQCLVPDDK
                     QDNPYLMWSISPGAAPRKIGLDPVAFDEGRYVIARNL"
     gene            complement(3073..4845)
                     /locus_tag="VF516_21325"
     CDS             complement(3073..4845)
                     /locus_tag="VF516_21325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110293.1"
                     /translation="MAVSPTSGLPRLQLKGIQRAPAPAGTVDIAAPAGGVNLPGFIDT
                     SDGANLRTGPAEAGGKTVLDAPLPPATRVFVSGTHPSAPEWWYVTALLPDKIVRGYVQ
                     EFRVNTDLPEPLAKLHQVKPGDTVEKLAVQEYASSVRDGHDLRYYENVLLFANKQRGR
                     VGIAGMYQDPGLLGGGANNIQLVAGHRIWLVSPAYAHAVEGVVPDGSLTNGAYAKVKR
                     FVQHVIDIIKSVTQSPHYFGEVGGEYAQAIRDHLPEIIGIVAGFIAAEAASAFLAATP
                     TGVGQIAAVLIQLALSAFGAVGMVEAGVEALKHASRWLTLAWTAQGKDEQIGAASREF
                     LRMLVSVAMAALAYTGVKGNLGKAVKIAQAMPPAGMVPALAGAGGRDGLMARAGEAVK
                     IGVPSPAGALGTATAMMTKHGDGEGPEPTGTDNATHSEAKGRKGSPEIETPKTEEPAT
                     SPQGQTADMSSASTESAALRGPARRHWAATVNQSTVAKNLNTVIEPGVDVAGDVAGIQ
                     AGKAARVNGNYVIHGRMYGVHDGTLYPISGPGFHQLNRGAFQALGVLNKLGNTPQAHT
                     ILERMKNVGPAEIEAALKMWSASR"
     gene            5217..5975
                     /locus_tag="VF516_21330"
     CDS             5217..5975
                     /locus_tag="VF516_21330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110294.1"
                     /translation="MRTVADPAAAVIGAQERLCVVLAPAGFGKTTTALRAIERAGGLA
                     LYIPATLLTDATPAVTALIASVVEFDGFLDEDRAAIEPLLRPVLKHLLKQRDLPVVVL
                     LDGLDESIRLTRRGGMHWLFGQLRDIEVPIVLGCRAEFWQDRLADFRTSLGEPAASGA
                     KRVQEIRTVELLPWEAEQIAALATRFAGRTTDADARARLERFAQDAGVDAIRLFGDVP
                     RTPLFCASSSKHSRAETCLRGRAPRCLSNGHESR"
     gene            6065..6412
                     /locus_tag="VF516_21335"
     CDS             6065..6412
                     /locus_tag="VF516_21335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110295.1"
                     /translation="MSFLAMEEAARTMVEVVDGAVELMPSCLLEALRTTAPRLVDILA
                     EPTPLFLHSLLILAPPSPSGERRIQFTHRVFQEFFLARWTMRPGHLIHGALPPAVVAL
                     REQLEGRSSPQVP"
     gene            6640..7200
                     /locus_tag="VF516_21340"
     CDS             6640..7200
                     /locus_tag="VF516_21340"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8110296.1"
                     /translation="MVLGTLAAMIPGGAGADPATHPARVLGEVKFRFDSAALPANAPQ
                     LLDGAVRFAAAHPDQRVVLDAHCDPVGTPPYNVGLAVRRAESVRAQLVARGVPADQIV
                     LAIYGEDGARRASYADDRRVTLWPTRRPLAQVIDHTLAGKGTAVTWNKPLTTAQLESA
                     PQPVASRDASPPAGRVARSTARPAAR"
     gene            complement(7145..>8856)
                     /locus_tag="VF516_21345"
     CDS             complement(7145..>8856)
                     /locus_tag="VF516_21345"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8110297.1"
                     /translation="VGGPLVTAWLEHQAAAATVRSMAIFAVLLVAITWWLYRSLRALL
                     AIVLALGAAVALTLAAGELLGFSFTIVSSLVPLTVMITTLATLTYLHSRFIDQPDDVP
                     LAEHHVAALRNKFLPVTASHVAAAAGFAALAVSRIRPIREMGIWTAVGLVLSWIVAYT
                     LFPVLQRTLRTPTRHRVAVRSALYDRISAALPRFTFRHRGSLVGAALAVCAAGAIAIT
                     GLPGVIAPIPLRVDTLSSIDPGTQLYRDLVWFRDHVTDLNIARIWIHLPRPTATDPEV
                     LRAIDRLTSEIEAAPDVTGVTGPTTPLRMRSYFAGHGERLPAEPARFAEAVGDVELLL
                     LSQPDLRGYIDVNHLRDLQLTVLFRDGDARGYAELARRVGAAWDRVRAGTPALAGAEL
                     RVVGESLLQVKVGASLVPTLAESFALTVALIFVVFLVVFRSGVERLLAMIPSLFALLA
                     SFLGLRLLGGSLNVATIIIATTVLGTTENDQLHFFHHMHERAGAGTEHQLRHALRVSG
                     RAVVFATIINATGFLGLSVARFPPLRQFGLLTAAAFVLALVGDFTVLPAALWIARRDR
                     PAD"
BASE COUNT         1377 a         2971 c         3056 g         1452 t
ORIGIN      
        1 cgtcgggccg tcgggctgcg gcaagtccac cttgctgaag atcctcgccg ggctgctgcg
       61 cccgaccgag ggcacggccg agctgcgcgg cgagccgatc accgggccgc ggcgcgacat
      121 cggcgtggtg ttccagaccc ccgtgctgtt tccgtggcgc acggtgctcg gcaacgcgat
      181 gctgccggcc gacgtccagc ggctcgaccg gcgggcgatg cagcagcgcg cgctcgagct
      241 gctcggcatg gtcggcctcg gcgggttcga gcgccgctac ccgcgcgagc tgtccggcgg
      301 gatgcagcag cgggtcggca tcgtgcgcgc gctgctccac gatccggcga tcctcttgat
      361 ggacgagccg ttcgccgcgc tcgacgcgat gacccgcgag gcgatgagcg tcgagctgca
      421 gcggatctgg ctgcagcgcc ggatcaccgt cctgttcatc acccactcga tcgccgaggc
      481 ggtgttcctg gccgaccggg tgatcgtgct gacgccgcgc cccgggacga tcggcgacca
      541 gttcgaggtc gatctgccgc ggccgcgcgc gctcgaggtg atgaacaccg agcggttcgg
      601 tcagcatgtg cggcgggtgc gcgacgcgct cggcggccgg gggacggtcg aatgacgcgg
      661 ggccccctgg cgttcgtgct gcgcctgatc ttcctcgccg cgctgatcgg cctgtgggag
      721 ctcgtggtgg tggagctcga cgtgccggcg ttcatcgtcc cggcgccgag ccaggtcgcc
      781 gtcgcgctgt accgcgggat cgagagcgat atttacacga cgcacctgta cgtgacggtc
      841 cacgagacgc tgttcgggtt cgcgctcggc tgtgcgctgg cctttgtgtc cggcagcgtc
      901 atcgcgctgt cgcgcaccgt ggagtactac ctctatccgc tgatcgtgat gttccagtcc
      961 gtgcccaagg tggcgctggt cccgctcatg atgacctggt tcggcgtcgg cgtcacctcc
     1021 aaggtggtga gcgcggcgct ggtggcgttc tttccgctga tggtcaatac cgtcgtcggg
     1081 ctgcgctcgg ccgacgagga ccgcgtcaac ctcctgcgct cgctggcggc gtcgcgctgg
     1141 cagatcttcc gcatgctgca gctgccgagc gcgctgccgt tcctgttcgc cggcctggag
     1201 gtcgcgctga tcttcgcgct gatcggcgcg atcgtcgccg agctgatcgg cgcggagaag
     1261 ggcctgggga tgctgaccca gagcatgtcg ttcagcatgg acacggccgg gcagttctcg
     1321 atcctgttca tcctcgcgat cctgggcctg gtcctgaacg ggctgctccg cctggtgcgg
     1381 cgcgaggtcc tgttctggga ccgctcgcac gacgccggtg tgctgtcgcc aacgaaagga
     1441 gatgatccgt gatgaagcgt attgccatcg cctgtgtgct ggtgctcgga gcgctggccg
     1501 cacccgcgga cgccgacgtg cgaacggtgc gcgtcggctg gtgcgccaag accgtgagct
     1561 cggctgccgc gccgttcgcc atcgcgcaga agctgggctg gttcgagaag gccgggatca
     1621 aggtcgaact ggtcccgctc cccggctcga ccgattgcgt gaagctgatg gcgacccgcg
     1681 acgtccaggt ctcgctggcc tcgatggagc cgctcgcgat cgtccggccc cagggagtga
     1741 aggcgaagtt cttctacacc gcctatcagg gcaacatcta tggcatcgcg gtcccggcgg
     1801 acagcccggt gaaatcgatg gccgatctca agggcaagcg gatcggcgtg atctcgatgg
     1861 cgtcggccgg cgtggtcgtc gcgcgcgcgc tggccaagca ggccggcctc gacccggaca
     1921 aggacatctc gatcgtcgtc gccggggaga gcgcgcagac cgcggcgctg ctgcggtcca
     1981 agcaggtcga tgcgctgtcg cagttcgaca cccagtacgc gctgaccagc aacgccggcg
     2041 ccaagctccg cttgctcgat caccccgaga tccggaagtt cccgtccaac gggttcgtcg
     2101 cgctcgagga ctatctcaag accaaccggg cggacgcggt ggcgctggcg cgcggctatg
     2161 ccatggggac ggtgtttgcg atcgcgaatc ccgaggcggc gattcgcatc ctgtgggagg
     2221 tgtatccgca gacccgggcg ctcggcaagg acgaggccac cgcgctcaag gacgacctgc
     2281 tcaccctgga tgcccgggcc aagcactggc gctacgagcc ggtcgacggc aagcgctggg
     2341 gcgacaacgt cgaggccaac taccgggcct acctcgactg gttgatcgca cagggcgtcg
     2401 tcaagacccg ggtcgacgtc aaggacctga tcaccagcga gctgctcgac gacgtgaaca
     2461 agttcgacgc cgaggcgatt cgccgggacg ccagggcgtt caaggcgaag tgacgcgacc
     2521 tcgagatttt gccgaattca aacttgcacc catggcaaca ttggaaactc gcgttcttct
     2581 ctgaacgagt cggtcttggc gaagcctcaa agctctcgag tacacctcgt gatcttcgca
     2641 gagaagtcga caagatctat aaatttcgag caatgacata ccgaccttcg tcgaaggcga
     2701 ccgggtcaag gccgatcttg cgaggcgcag ctccaggtga aatcgaccac atcagataag
     2761 gattatcttg tttgtcatct ggaacaagac attgcgtccc caaggcatcg cagaggcgcc
     2821 gcatgagctg ctcatgcgtt tcgcaaggca ccgcgaggcg ttcgacttga atggatagca
     2881 gaccagtgaa ttctcctttg agcgctgaag acacacacac gatatcggcg gcaccggcct
     2941 caggatagtt gctcaggtcg tcgatcacga caacgcgttc ctcggaaacc gccagcgctg
     3001 ctgcgagcgc tcgccgtacg ctggcatcag gagaacgatg ttgaacgact atgtctatcc
     3061 cattgaattc ggtcatcgcg acgcgctcca catcttcaaa gcagcttcga tctccgccgg
     3121 tcctacgttc ttcatccttt cgaggatggt atgagcctgt ggagtatttc cgagcttgtt
     3181 gagcacacca agcgcttgaa acgctcctcg gtttaactga tggaaaccag ggccggagat
     3241 cggatacagc gtaccatcgt gaactccata catccgtccg tggatgacat aatttccatt
     3301 cacgcgagct gccttccctg cctggatccc ggcgacatca ccggcaacgt ccacgcccgg
     3361 ctcgatgacc gtgttgaggt tcttcgcgac ggtgctctga ttcaccgttg ccgcccagtg
     3421 ccggcgagct ggtccccgca gcgcggcgct ctcggtcgaa gctgacgaca tatctgcggt
     3481 ctgtccctgc ggggacgtcg ccggctcctc cgtcttcggt gtctcgatct ccgggctacc
     3541 cttccttcct ttggcctccg agtgtgtcgc gttgtcagta cctgttggtt cagggccttc
     3601 cccatctcca tgcttcgtca tcatggcagt ggccgtaccc agcgcccccg ccgggctggg
     3661 cacgccgatc ttgactgctt cgccggccct ggccatcagc ccatccctgc caccggcgcc
     3721 cgcgagcgcc ggcaccatgc cggccggagg catcgcctgc gcgatcttga cggccttgcc
     3781 caggttgccc ttcacgccgg tgtacgcgag cgccgccatc gcgacgctga ccagcatacg
     3841 caggaactcg cggctcgcgg caccgatctg ctcgtccttc ccctgcgccg tccatgcgag
     3901 cgtcaaccac cggctggcgt gcttcagcgc ctcgacgccc gcttctacca tcccgaccgc
     3961 gccgaacgcc gagagcgcga gctggatcag cacggcagcg atctgaccga cgccggtcgg
     4021 cgtggctgcg aggaacgccg atgcggcctc ggctgcgatg aagcctgcga cgatgccgat
     4081 gatctcgggc aggtgatcgc ggatcgcctg cgcgtactcc ccgccgacct cgccgaagta
     4141 gtgcggcgac tgcgtgacgc tcttgatgat gtcgatgacg tgctggacga accgcttgac
     4201 cttcgcgtac gcgccgttcg tcagagagcc gtcgggaacg acgccctcga ccgcatgcgc
     4261 gtaggccggg ctcacgagcc agatgcggtg cccggcgaca agctggatgt tgttggcgcc
     4321 accgcccagc agccccggat cttgatacat gccggcgatt ccgacgcgcc cgcgctgctt
     4381 gtttgcgaac aacagcacgt tctcgtagta gcgcaggtcg tggccatctc gaaccgacga
     4441 tgcgtactcc tggaccgcga gcttctcgac ggtgtcgccc ggcttcacct gatggagctt
     4501 ggcgagcggc tcgggaagat cggtgttgac gcggaactct tggacgtacc cgcgcacgat
     4561 cttgtcgggc aggagcgcgg tcacgtacca ccactcgggc gcgctcggat gcgtgccgct
     4621 cacgaagacg cgcgtcgcgg gtggcagggg cgcgtcgagc accgtcttgc cgcccgcctc
     4681 cgccggcccg gtgcgcaggt tggcgccgtc gctcgtgtcg atgaatccgg ggaggttgac
     4741 gccgccggca ggcgcggcga tgtcgacggt gcccgccggg gccggtgcgc gctgaatgcc
     4801 cttgagctga aggcgaggca acccgctcgt cgggctgacg gccacgtccg gcgccgccgt
     4861 aggtgccatt gcatccggcg gcaaaacggc cgccccagcg tgtgcgggcg acgaagccgt
     4921 tagcggcgcc gccaaacccg ccacttgttc gacgagcgtc cgcataccga cagccggcga
     4981 tcctgaacga tcgccctcgc gatgctgcgt cgtactcgtg cctctcccga ccttgccctg
     5041 cacgctccga gaagctagcc ggggggcgga cgcgactcaa ggcccttcgc cgggatctgt
     5101 tagctcacgc ttgccttgct caactaccga gcggcccgct ggagaagccg gtggtaccac
     5161 tacccaccac cgcggccatg cggcgtgccc gtgaccctcg tgctgcatcc gatgaggtgc
     5221 gaaccgtcgc ggacccagcg gccgcggtga tcggtgcgca ggagcgcctg tgtgtggtcc
     5281 ttgcgccagc cgggttcggg aagacaacga ccgcgttgcg cgcgatcgag cgtgccggcg
     5341 ggctcgcgct ctacattccc gcgacgctgc tcaccgatgc caccccggcc gtcacggcgc
     5401 tcatcgccag cgttgtcgag ttcgatggat ttctcgacga ggatcgggcg gcgatcgaac
     5461 cgttgctgcg ccccgttctc aagcacctgc tgaagcagcg cgacctgccg gtcgtcgttt
     5521 tgctcgatgg gctcgacgaa tcgatcaggc tgacccgacg gggtgggatg cactggttgt
     5581 tcggacagct ccgagatatc gaggtaccga tcgtgctggg atgccgtgca gagttctggc
     5641 aagatcggct tgccgacttt cggaccagcc tgggcgagcc tgctgcatca ggagcgaaac
     5701 gagtgcagga gatccgaact gtcgagctac ttccctggga agcggaacag atcgctgcgc
     5761 tggcaactcg attcgctggt cgcaccacgg acgccgacgc acgggctcgg ctcgagcgct
     5821 tcgcgcagga tgcaggagtc gatgcgatcc ggcttttcgg cgacgtcccg cggacaccgc
     5881 tattttgcgc ctcatcctcg aaacactcga gagcggagac gtgtttacgt ggtcgcgctc
     5941 cgcggtgctt gagcaatggg cacgaatcaa gatagagcga gacgtccgcg cgccgctcgt
     6001 ctggggtggg cctggtcgtc ggtacctggt gtcgcccgac gagtcggtgg acacggtcgt
     6061 tgagatgtcc ttcctcgcga tggaggaggc ggctcgcacc atggtggagg tcgtcgatgg
     6121 cgcggtcgag ctcatgcctt cgtgtttgct cgaggcgttg cgcaccaccg ctcctcgact
     6181 cgtggatatc cttgccgagc caacgccgct gttcctgcac agcctcctga tcctcgcccc
     6241 accttcgcct tccggtgagc ggcgtattca gttcacccac cgcgtgttcc aggagttctt
     6301 cctcgcacgg tggacgatgc ggcccgggca cctcattcac ggggcgctac ctccagccgt
     6361 cgttgcgctg cgcgagcagc tcgaagggcg atccagtccc caagttcctt gagccccgat
     6421 tcagtccggc cggggcccag accgggagcc tgtccggggt gacgcggacc gcctgatcgg
     6481 tgcggcgtgt ccgcacggcg gccgcggatg cggtccggca cgcgtgtgcg catgcgcgcg
     6541 gatcgcggag cctgtgcgcc gaggatccgc ggagatcgac gagcggcatg cacgatgcaa
     6601 tgtgcagagt gtgtcatgaa gacgctaatg ccaatctcca tggtcctcgg aacgctcgct
     6661 gcaatgatcc cgggtggcgc tggcgcagac ccggccacgc accccgcgcg ggtgctcggc
     6721 gaggtgaagt tccggttcga ctccgccgcg ctgccggcga acgcgccgca gctgctcgac
     6781 ggcgcggtcc ggttcgccgc cgcacacccc gaccagcgcg tcgtgctcga tgcacactgc
     6841 gatccggtcg gcacgccgcc gtacaacgtc gggctggcgg tccgccgcgc cgagtcggtg
     6901 cgcgcacagc tggtcgcgcg cggcgtgccg gcggaccaga tcgtgctcgc gatctacggc
     6961 gaggacggcg cgcggcgggc gagctacgcc gacgaccggc gggtgacgct gtggccgacg
     7021 cgccggccgc tggcccaggt catcgaccac acgctcgccg gcaagggcac ggccgtgacc
     7081 tggaacaagc cgctgaccac cgcccagctc gagtcggcgc cgcagccggt ggcgtcgcgg
     7141 gacgctagtc cgccggccgg tcgcgtcgcg cgatccacag cgcggccggc agcacggtga
     7201 agtcgccgac cagcgcgagc acgaacgcgg cggcggtgag caggccgaac tggcgcagcg
     7261 gcgggaaccg cgccaccgac aggccgagga agccggtggc gttgatgatc gtcgcgaaca
     7321 ccaccgcgcg gcccgagacc cgcagcgcgt ggcgcagctg gtgctcggtg cccgcgccgg
     7381 cgcgctcgtg catgtggtgg aagaagtgga gctgatcgtt ctcggtggtg ccgagcacgg
     7441 tggtcgcgat gatgatcgtg gcgacgttga gcgatccgcc gagcaggcgc aggccgagga
     7501 agctggcgag cagcgcgaac agcgacggga tcatcgcgag caggcgctcg acgccgctgc
     7561 ggaacacgac gaggaacacc acgaagatca gggccacggt cagcgcgaag ctctcggcga
     7621 gcgtcggcac caggctcgcg ccgaccttga cctgcagcag cgactcgccg acgacgcgga
     7681 gctcggcgcc ggccagcgcc ggggtgccgg cgcgcacccg atcccacgcg gccccgaccc
     7741 ggcgcgcgag ctcggcgtaa ccccgggcgt cgccgtcgcg gaacagcacg gtgagctgga
     7801 gatcgcggag gtggttgacg tcgatgtagc cccgcaggtc gggctgtgac agcagcagca
     7861 gctcgacgtc gccgaccgcc tcggcgaacc gcgcgggctc ggcgggcagg cgctcgccgt
     7921 ggccggcgaa gtagctgcgc atgcgcagcg gcgtggtcgg cccggtgacg ccggtgacgt
     7981 cgggcgccgc ctcgatctcg ctggtcagcc gatcgatcgc gcgcagcacc tccggatcgg
     8041 tggcggtcgg gcgcggcagg tggatccaga tccgggcgat gttgagatcc gtcacgtgat
     8101 cgcggaacca cacgaggtcg cggtacagct gggtccccgg gtcgatgctg gacagcgtgt
     8161 cgacccgcag cgggatcggg gcgatgacgc cgggcagccc ggtgatcgcg atcgcaccgg
     8221 cggcgcacac cgcgagcgcc gcgccgacca gcgacccgcg gtggcggaag gtgaaccggg
     8281 gcagcgcggc ggagatccgg tcgtacagcg cgctgcgcac cgcgacgcgg tggcgggtcg
     8341 gcgtgcgcaa ggtgcgctgg agcacgggga acagcgtgta cgcgacgatc cacgacagca
     8401 ccaggccgac cgcggtccag atccccatct cgcggatcgg gcggatccgc gacaccgcga
     8461 gcgccgcgaa gccggcggcc gcggcgacgt gggacgcggt gaccggcagg aacttgttgc
     8521 ggagcgccgc gacgtggtgc tcggcgagcg gaacgtcgtc gggctgatcg atgaaccgcg
     8581 aatgcaggta ggtcagcgtc gccagcgtcg tgatcatcac ggtgagcggg accaggctgg
     8641 acacgatggt gaacgagaag ccgagcagct cgccggcggc gagcgtgagc gcgaccgcgg
     8701 cgcccagcgc gagcacgatc gcgagcagcg cgcgcagcga gcggtagagc caccaggtga
     8761 tcgcgaccag caggaccgcg aagatcgcca tcgaccggac ggtcgccgcc gccgcctggt
     8821 gctcgagcca cgcggtcacc agcggcccgc cgacct
//
LOCUS       DASVOR010001117         1795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_157274,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001117 DASVOR010000000
VERSION     DASVOR010001117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1795
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157274"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1476)
                     /locus_tag="VF516_21350"
     CDS             complement(94..1476)
                     /locus_tag="VF516_21350"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEX8110298.1"
                     /translation="MTTASGDPTQLTLTEIGAAYRARQLSPVEATQACLDRIARLDPE
                     LHAFITVTGELALDQARAAQVELGAGRDRGPLHGVPIALKDLIDTAGVRTTGGSALFE
                     DRVPAEDAEVVRRLRDAGAVVLGKLNLHEMAYGASGVIGHFPTTRNPRNPAHITGGSS
                     SGSAAAVAAGMCYAALGSDTAGSIRLPAALCGIVGFMPSYGLVSLRGVIPLAWSYDHV
                     GPMTRTVRDAAVVLQAIAGYDAGDITSIAFPAADYIAAVDGAAAGPKLRLGIARSHFF
                     ADLDPEVAAAVERALGVLVGLGATLRDVEVAVDEDRTVARAETWAYHRAWVDKAPERY
                     QPDTLRRIRTGEAVTGADYVDRWRDLQRMRRAAPALFTDVDLMVMPTTPMPAPTLAEV
                     MARPDELRPRELVLMRNTRMFDVLGTAAISVPCGTTAAGLPIGLQIIGAPGADAAVLR
                     LAAAYEAATR"
BASE COUNT          237 a          660 c          670 g          228 t
ORIGIN      
        1 ccagcgtcac cggcggctcg ctcggcctcg ccgtcggcgg cgcgttctga tcgggggacg
       61 gcggggagaa ccggctctga ccgcgggagc cgttcagcgc gtcgcggcct cgtacgcggc
      121 ggccagccgc agcaccgcgg cgtcggcgcc gggcgcgccg atgatctgca ggccgatcgg
      181 cagccccgcg gcggtggtgc cgcacggcac gctgatcgct gccgtgccga gcacgtcgaa
      241 catccgcgtg tttcgcatca ggaccagctc gcgggggcgc agctcgtcgg gccgggccat
      301 gacctcggcc agggtcggcg ccggcatcgg tgtggtcggc atgaccatca ggtcgacgtc
      361 ggtgaacagc gccggcgcgg cgcgccgcat gcgctggagg tcgcgccagc ggtcgacgta
      421 gtccgcgccg gtcaccgcct cgccggtccg gatccggcgc agggtatcgg gctggtagcg
      481 ctcgggtgcc ttgtcgaccc acgcgcggtg gtaggcccac gtctcggcgc gggccaccgt
      541 gcggtcctcg tcgaccgcga cctcgacatc gcgcagcgtg gcgcccaggc cgacgagcac
      601 gccgagcgcg cgctcgaccg cggcggcgac ctcggggtcg agatcggcga agaagtgcga
      661 tcgcgcgatg ccgagccgca gcttcggccc cgccgcggcg ccgtcgaccg cggcgatgta
      721 gtcggcggcc gggaacgcga tgctggtgat gtcgcccgcg tcatagccgg cgatcgcctg
      781 gagcacgacc gccgcgtcgc gcaccgtgcg cgtcatcggt ccgacgtgat cgtacgacca
      841 cgccagcggg atcacgccgc gcaggctgac cagcccgtag ctcggcatga acccgacgat
      901 gccgcacagc gcggccggga gccggatcga gcccgcggtg tccgagccga gcgccgcgta
      961 gcacatcccc gcagcgaccg cggcggccga gcccgacgac gatccgccgg tgatgtgcgc
     1021 cgggttgcgc gggttgcgcg tggtcgggaa gtggccgatc acgccgctgg cgccgtacgc
     1081 catctcgtgc aggttgagct tgcccagcac caccgcgccc gcgtcgcgca gccggcgcac
     1141 gacctcggcg tcctcggccg gcacccggtc ctcgaacagc gcgctgccgc cggtggtgcg
     1201 gactccggcg gtgtcgatca ggtccttgag cgcgatcggc acgccgtgca gcggcccgcg
     1261 atcgcgaccg gcgccgagct cgacctgggc cgcgcgcgcc tggtcgagcg cgagctcacc
     1321 ggtgaccgtg atgaacgcgt ggagctcggg atcgagccgc gcgatccgat cgaggcaggc
     1381 ctgggtcgcc tcgaccggcg acagctgccg cgcgcggtac gccgcgccga tctcggtgag
     1441 ggtgagctgc gtcggatcgc cggaggcggt ggtcatggcc gcactctaca ccgccccgcc
     1501 gctgcgctcc gggtcaggag cggaatccca gtcgctccag agcgccgcgt cagcgaggct
     1561 cgcgagatcg agcgcccaga tccgtgaccg atgccggcga gctgcccgag cgctcagcgg
     1621 cgccgaaagc agggacctca tcccgagcgc tcagccgcgc cgaaatacat cgcgaacggt
     1681 ccgcgcagtc acggcggtct ccagccacgc ttcgagccgt gtggtgtctg tctcgcgctc
     1741 gatacgggcc cgcatggccg aggtcaccga gaacgaacgc gccgcgagca ccttg
//
LOCUS       DASVOR010001118        11103 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_216058,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001118 DASVOR010000000
VERSION     DASVOR010001118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216058"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..975)
                     /locus_tag="VF516_21355"
     CDS             complement(<1..975)
                     /locus_tag="VF516_21355"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8110299.1"
                     /translation="MQRRIRLAERTHAVTPFLAMEFSKHAAVLEAEGHRIIQLNVGQP
                     DFGAPPAVVRAMRAALDENRTSYTPALGIWELRRAIAHHYATTHGVELDPGRVIVTAG
                     ASAALLLLTAALVDPGDEVLVADPSYPCNRHFLSSFGAEVKLVPTTPATRYQLDAALL
                     RQHWGPRTRGVMVATPSNPTGTSVREAELAAMCRYADERDGWRIVDEIYLGLQDGKPR
                     TALASDGDALVINSFSKYFGMTGWRLGWCVVPGDMVPVLERLAQNYYICPSAPAQYAA
                     VACFDDESIAICEDRRAQLTRRRGLVVQGLKAIGLDVPVVPDGAFYVYI"
     gene            complement(1017..2621)
                     /locus_tag="VF516_21360"
     CDS             complement(1017..2621)
                     /locus_tag="VF516_21360"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8110300.1"
                     /translation="MWTSRQILFQLGAELGSCAGVELEFSVRLGPGRIEPAGRFGCWL
                     PGSSGHDFAPVLAALQRLEAPEEVMQAQRQAMLPVRQGIAVALHDGSLELRLYLHGRD
                     PRTLADHYCAWRWSSGEAVRRSHYEFHFLPESPSGLRPLDLVAVELQPYLTRLLAMDR
                     LQQSSGFWLRRGEDSRLEQLDLALPWNPTVGSLPGLQELVEKLAIPTDAFADLRDLPV
                     RHLALSLDPGPIAVTLYASAPVAGDWPETEDALQHQVRRGARQFQSEVEDRIYRQLPA
                     MPAAGADAAELGAFYDGEVSRWQKVLGHELHYHAGLFAAPGEQPDDEEMTLALRRAVT
                     ELYPFLPAGGRVYDIGCGWGGPLAMMVGDLRCRCLGLTISRTQYRHAASLGLPVRLGD
                     AEMTWPPGLFDCALLLESFSHVRDKARLLRVLRPFVGRLVMRVNCQDASPPGPAFGGT
                     MHMIRSSQLRTLLEAAGWQIKHWRDRRMEALPSVAVWLRRLQLVPPGDDRHLETLRAW
                     CERIMRGPQEWGRNNPLIEVVADRSP"
     gene            complement(2696..7459)
                     /locus_tag="VF516_21365"
     CDS             complement(2696..7459)
                     /locus_tag="VF516_21365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110301.1"
                     /translation="MTDIKNLELLKSRLEASHPVDGRLTLKPEMIPDGDFLAFLRTLP
                     QQQLVLEDPKVTPTGSPAITRLDVAGQVRGSWTVPFVNGGLLSSIQVKISFLQTSASG
                     PILSTLAISQATLALAGKPISVSGSTLPGTLLLTLTLLPTGPHKQVFSLHDALAFLSG
                     SSSSLAPQGISLFDQIQLSGVQLSFGFAPGSSTYFEISADANGDWDIIDGGLFALKGV
                     GVSVLAVHRPAKAGGADLSLAGNVHATTRIASSDFQILLYLLGRNRLEFDVLPSSTQV
                     LPGLAELAGHLGGEALKSAVQDGVKALGLGDISIDRIRVGFDLVHRKLLYVTLQSHLT
                     LAGGQIFLYTQLPDFSFYGGLPPAIKGQPAAPSRISVQALATHVFGEAADLPELAITQ
                     LCLFAYPGQGTYRIEVLTEEDWKWDPTGRGTGPFLALENLGVNLERSPQSVSGGITVG
                     ANLGGFEIVFNAQYLGKGLGWLFTGLGATEHGVPVGTFLQDVAQKIGASVPPALLQAV
                     QSLEIHQLTVVFDTHQKSFQFTADTTTTGQIPLGPRSYELDTRVFLTSTLDPSTGQRV
                     FTGHMEADLQIGKALFLVSYDLGANVKTFIGRWRTSDGSTIGFNDLAEALGIEHTISV
                     PDGLDLGLQAASFEYHAADNFFTLAAESSHHGDAFFTAGKGQTGTWGFVFGIDFPPKA
                     KLSSLPVIGSDLKVADFLTFQQFAILLSSTTFKNYTLPVLPPLPAPSPSATSPGVGRL
                     VGGRSVKPIAAGASLQVSPGLSLAAMLDLAASSSDARMRNLASIVGKPELLVQVTMGE
                     SGVSLFIALAGAVSIPAGGTSKLTLGSPAMLIVLSTEIVFQLVGSIELSVNGAAVDAT
                     VRMILDESEAQVTVDISGDHGSLPSPPGVKGLHLTDFGIAMGVFFEPPGVDLGILGKL
                     RIGEVQGTRSDEFAIVLEMIEEIPDLLYLSFYIDKLDLGQVVTLFTDKSEPASVQALE
                     VVKASDLSFYFAENVIVLPDGTIAQPGFGVRASIQMFSFGAHADLEIGLTQGLKGHAE
                     MAPINLRNVLRISGDGKGIHRTYQQIDGQWQLVNNNSIVRQKPAPPTRQEVVVPPGGP
                     IIEFDALRSPFIHVNWQITLFDLEKTRVDVTVSNKGASFLLSYQVAGIEKFDLNATLQ
                     DLEHFSASAAFHLGLDARVGPIHVLGVDCGSLHLVARADAGMELVLDPSRFSLRIHGD
                     FDFEGIKFSIPDLTLSVAPASLKELPEKIIQQIKDHADQMFAALFADAQKWAVMIARG
                     VITNVSNMARALKNVYKVTAEDAARLMKAANQAVNTVASGLQTAYGLTGDAATAAMKG
                     AGYAAEQIADGLKQAYGLTAQGAITVLKGAGFAAGEVGNALKTAYSQAAQQATVLLRG
                     AGYAVGDIGHALAAAYGASANQAAQLLKGAAYAANEVGSALQSAYGATASVATGALKG
                     AGYAAGEVGGMLRSVYGQTAQQAASVMRSAGYAADQVGTALKSAYGVTVDQAAVILRG
                     AGYGINDVGNALKSAYGATADVAASALKKAGYAVNETGDFAKNAYNLGPDRLKSVLSN
                     AGYAGDQIKDFFHSLGGAFADAFKDVGKKLDPTHW"
     gene            complement(7496..8734)
                     /locus_tag="VF516_21370"
     CDS             complement(7496..8734)
                     /locus_tag="VF516_21370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110302.1"
                     /translation="MEIWHIDVVGSGDSTLIIATDDGVGAGHVAMTRSALIDGGRTSY
                     ANHVHNRITTQGLAALDVMVATHYDEDHYGGLRSLLNMGNAIYANTRVYDQGEPGMVT
                     AKRDRAQNWRWYVSGRDPQYQAYVNAVGARVRPTGRVLAGRSGPGVSHAGWHDPDWLV
                     GTEILLPPAPVPRGPGAPTLTCVAANQYVLRAAGAGTRLVSAAVLGDQLKNAKSLAFL
                     LRFGNFKYYVGGDIVAVQEDGSQWNGNGYTVAQANRNNSLMQYLNQGNNVANRVHALK
                     TSHHGSKYSSSPAFLARLRPRAAFISCGTDNQYDHPDQQVVTALHNDPTVRQYFLTGE
                     WLNGTANLTNFATVAGVWLPGTAAHTHRGDLCLGLTAAQAGAALVQFTVDFFSPNAGT
                     NIHKNPAFAYAPFGAFQMPF"
     gene            complement(8938..9399)
                     /locus_tag="VF516_21375"
     CDS             complement(8938..9399)
                     /locus_tag="VF516_21375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110303.1"
                     /translation="MTGPAGHRLLIAGLALALAAPGAARADVTADCDFLEISARAGDK
                     PAVDGELKPLEKKLKKPPFSTWNQFKLLGRTQKALAKKKQEPIPLKIGSATATLVEIV
                     DKSKVRLTVTMNDARGKEVANSTSTVEAGDYLMYVHSLPGNEGHLLSVSCK"
     gene            complement(9396..9938)
                     /locus_tag="VF516_21380"
     CDS             complement(9396..9938)
                     /locus_tag="VF516_21380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110304.1"
                     /translation="MTRPPTSPSDLELMEHADGELDDPELIARLERDPDARVRLEAIQ
                     ELGDLVRGHLELSADTIHDARFAAMWRRIDAQLVAPTGLWARISGWFDRHRGHVITGV
                     VSAGAVAALAIVLRPGNSDLGASGAHAIEVRPVAQRAAPEIDSLDTPGGSSTVISLND
                     DDGNATVIWVTPQDDDAETL"
     gene            complement(9947..10687)
                     /locus_tag="VF516_21385"
     CDS             complement(9947..10687)
                     /locus_tag="VF516_21385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8110305.1"
                     /translation="MNKPGQRASISTHGRDPVDDRELVAAAQRGDRDAFKTLFERYHR
                     RAYALALGVVRHPDDALDVVQDAFIKAHKYLDKFEGNASFYTWLYRIVMNLAIDHLRK
                     HRKVKPVELDETRLEADGDDPLLPRILGGNPGQALIDKQIRARIDLALDQLSENHRAV
                     LVMRELEGLSYEDMAQAMSCSKGTIMSRLFHARRNMQKRLLDLLDHPPAELVEDLTGG
                     AAPAAPEDAPATSAPAHPLARAARSGTP"
     gene            10879..>11103
                     /locus_tag="VF516_21390"
     CDS             10879..>11103
                     /locus_tag="VF516_21390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110306.1"
                     /translation="MGLYATAAVAGIALVLPLVGVVVSASRATALAVLALGGLVVVLL
                     AVTAVVLGLIAPRRRWGGDPALARWVGVRRR"
BASE COUNT         1845 a         3623 c         3760 g         1875 t
ORIGIN      
        1 gatgtagacg tagaacgcgc cgtcgggcac gaccggaacg tcgaggccga tcgccttcag
       61 gccctgcacg accagcccgc ggcgacgggt cagctgggcg cgccggtcct cgcagatcgc
      121 gatcgactcg tcgtcgaagc aggccaccgc ggcgtactgc gcgggggccg acgggcagat
      181 gtagtagttc tgcgccaggc gctcgagcac cgggaccatg tcgcccggca ccacgcacca
      241 gccgagccgc cagcccgtca tgccgaagta cttcgagaag ctgttgatga cgagcgcatc
      301 gccatcgctg gcgagcgccg tgcgcggctt gccatcctgg aggccgaggt agatctcgtc
      361 gacgatgcgc cagccatcgc gctcgtcggc gtagcggcac atggcagcga gctcagcttc
      421 acggaccgag gtgcccgtcg ggttggaagg tgttgccacc atgacgcccc gtgtccgcgg
      481 tccccagtgt tgccgcagca gcgcggcgtc gagctggtac cgcgttgccg gcgtcgtcgg
      541 gaccagcttg acctcggccc cgaagctcga caggaaatgc cggttgcatg gatacgaggg
      601 gtccgcgacc agcacctcgt cgccgggatc gaccagcgcg gccgtgagca ggaggagcgc
      661 tgcggatgcg ccggcggtga cgatgacgcg tcccggatcg agctcgacgc catgcgtcgt
      721 cgcgtagtgg tgggcgatcg cccgccggag ctcccagatc cccagcgctg gcgtgtacga
      781 cgtacggttc tcgtcgagcg cagcgcgcat cgcgcggacc acggcgggcg gtgcgccgaa
      841 gtcaggctgg ccgacgttga gctggatgat gcgatgcccc tcggcctcga gcaccgcggc
      901 gtgcttgctg aactccatcg ccaggaatgg cgtgaccgca tgggtccgct cggccagtct
      961 gattcgtcgc tgcaagccgt gatcttggac gacctctcgc gacgacgcaa ccagcgtcac
     1021 gggcttcgat cggcgaccac ctcgatcagc ggattgttgc gaccccactc ctggggcccg
     1081 cgcatgattc gctcgcacca cgctcgcagc gtctccaggt gacgatcgtc gccaggagga
     1141 acgagctgca acctgcgaag ccagaccgca accgatggca acgcctccat ccggcgatcg
     1201 cgccagtgct tgatctgcca gccagctgct tccagcagcg ttcgtagctg cgaggagcgg
     1261 atcatgtgca tggtgccgcc gaacgcgggg ccgggtgggg aggcatcctg acagttgacg
     1321 cgcatcacca ggcggccgac gaacggccgc aggactcgca gcagccgcgc cttgtctcgc
     1381 acatggctga acgactccag cagcagcgcg cagtcgaaca gcccgggcgg ccaggtcatc
     1441 tcggcgtcgc cgaggcgcac cggcaagccg agcgaagcgg cgtgccggta ttgcgtacgg
     1501 ctgatggtca gccccaggca gcggcatcgc agatcgccga ccatcatggc cagagggcca
     1561 ccccagccgc agccgatgtc gtacaccctt ccgccagccg gcaagaatgg atacagctcg
     1621 gtcaccgcgc gtcgcagcgc gagcgtcatc tcctcatcgt cgggttgctc gccgggcgca
     1681 gcgaacagtc cagcgtgata atgcagctcg tgtccaagca ccttctgcca tcggctcacc
     1741 tcgccgtcgt agaacgcgcc cagctctgcc gcgtccgcgc cggccgcggg catggcagga
     1801 agctgacgat agatccgatc ctcgacctcg gactggaatt gccgggcgcc gcggcgcacc
     1861 tggtgctgca gcgcgtcttc ggtctccggc caatctcctg caacgggagc cgaggcatac
     1921 agcgtgaccg cgatcggtcc agggtcgaga gacagcgcca ggtgccggac cggaaggtct
     1981 cgcagatcag cgaacgcatc ggtcggaatg gccagcttct cgaccagttc ctgcaggccc
     2041 ggcaaggagc cgaccgtggg gttccagggc aacgccagat cgagctgctc cagccgactg
     2101 tcctcgccgc gccgcagcca gaagcccgag ctctgctgca ggcgatccat ggcaagcagc
     2161 cgtgtgaggt aaggctgcaa ctccaccgcg acgagatcca gcggacgcag tccggaagga
     2221 ctctccggca gaaagtgaaa ttcatagtga gagcgccgca cggcctcgcc cgagctccag
     2281 cgccacgcgc aataatggtc ggccagggtg cggggatcgc gtccatgcag gtagaggcgg
     2341 agctcgagac tcccgtcgtg tagcgcgact gcgattccct ggcgcaccgg cagcattgcc
     2401 tgtctctgcg cctgcatcac ttcctcggga gcctccagcc gttgcaaggc agccaggacg
     2461 ggcgcaaagt cgtgacctga gctacccggc agccagcaac cgaagcgccc cgcaggttcg
     2521 atgcgtcccg gcccgagccg gaccgagaac tcgagctcca cgccggcgca ggagccgagc
     2581 tcggcgccca gctggaagag aatctgccgg gaggtccaca cggatctagt acccaggcta
     2641 tgacatgatt cgttcggtgc cggctgggcc gaccgcgctg atcccttccg gtccatcacc
     2701 agtgagtagg atcgagcttt ttaccgacgt ccttgaacgc atcggcgaag gcgcccccca
     2761 gcgagtggaa gaaatccttg atctgatctc ctgcataacc agcgttcgac aacacactct
     2821 tcaatcgatc aggacccaga ttgtaagcgt tcttcgcgaa gtcaccggtc tcgttgaccg
     2881 cgtatccggc cttcttcagg gcgctggcgg ctacatcggc cgtggccccg taggccgact
     2941 tgagcgcgtt gcccacgtcg ttgatgccat agccagcgcc tctcaggatc acggcggcct
     3001 ggtcaacggt cactccatac gcgctcttga gcgcggtgcc gacctgatcg gccgcgtagc
     3061 ccgcgctccg catgacgctg gctgcctgct gcgccgtctg cccatacacc gatctcagca
     3121 tgccgccgac ctcgccggcg gcgtatccgg cgcccttgag cgcgccggtc gcgacgctgg
     3181 ccgtggcgcc ataggcggac tggagggcac tgccgacctc gttcgcagcg tacgcggcgc
     3241 ccttgagaag ctgtgcggcc tggtttgccg aggctccgta cgcagccgcg agcgcgtggc
     3301 cgatatcacc gaccgcatag cccgcgcctc tgaggagcac agtggcctgc tgtgcggcct
     3361 ggctgtaggc ggtcttcaag gcgttgccca cctccccggc ggcaaacccc gcgcccttca
     3421 ggacggtgat ggcgccctgc gccgtgaggc cgtatgcctg cttcaatccg tccgcgatct
     3481 gctcggcagc atagcccgcg cccttcatcg ccgccgtggc cgcgtcgccc gtcaggccgt
     3541 aggcggtctg gagacccgag gccaccgtat tcaccgcctg gttggcagct ttcatcagcc
     3601 gcgccgcgtc ctcggccgtg accttgtata cgttcttcag ggcacgggcc atgttgctga
     3661 cgttcgtgat cacaccgcgc gctatcatga cggcccattt ctgcgcatcg gcaaacagcg
     3721 cggcgaacat ctggtcggcg tggtccttga tctgttgaat gatcttctcc ggcagctcct
     3781 tgagcgatgc gggtgccaca ctcagggtca ggtctggaat cgagaacttg atgccctcga
     3841 agtcgaagtc tccgtgaatg cggaggctga agcgcgacgg atcgaggacc agctccatgc
     3901 cggcatcggc cctggccacc aggtgcaggc taccgcaatc cactccgagc acgtgaatgg
     3961 ggccgacgcg agcatccagt ccgagatgaa atgcagcgct ggcgctgaag tgctccagat
     4021 cctggagcgt ggcgttcaga tcgaacttct cgatccccgc gacctggtag gacagcaaga
     4081 acgaagcgcc cttgttggag acggtgacgt cgacccgcgt cttttccaga tcaaacagcg
     4141 tgatctgcca gttcacatgg atgaatggcg agcgcagcgc gtcgaactcg atgatcggac
     4201 cacccggcgg gacgaccacc tcctgtcgcg tgggcggcgc cggcttctgg cgaacgatgc
     4261 tgttgttgtt gaccagctgc cactgcccgt cgatctgctg gtacgtgcga tggatgccct
     4321 tgccatcgcc cgagatcctc agcacgttgc gcaggttgat gggagccatc tccgcatgac
     4381 ccttcagccc ctgggtcagg ccgatctcca ggtccgcatg cgcgccgaac gagaacatct
     4441 ggatgctcgc gcggacgccg aaccccggct gcgcgatggt gccatccggc aacacgatca
     4501 cgttctcggc gaaatagaag ctcaggtccg atgccttcac gacctcgagc gcctgcaccg
     4561 aggcgggctc cgacttgtcg gtgaacagtg tgaccacctg ccccaggtcc agcttgtcga
     4621 tatagaacga gaggtagagg aggtcgggaa tttcctcgat catctccagc acgatcgcga
     4681 actcgtcgct ccgggtgccc tgcacctcac cgatccggag cttgcccagg atccccagat
     4741 cgacacccgg gggctcgaag aacaccccca tcgcgatacc gaagtctgtc aggtgcagcc
     4801 ccttcacgcc cggaggcgat ggcagcgagc cgtggtcgcc cgagatgtcg acggtgacct
     4861 gggcctccga ctcgtcgagg atcatgcgga ccgtggcgtc gaccgccgct ccgttgaccg
     4921 agagctcgat gctgcccacg agctggaaga cgatctccgt ggacaggacg atgagcatgg
     4981 cagggctgcc cagcgtcaac ttcgacgttc caccggcggg gatgctcacc gcgccggcca
     5041 gcgcgatgaa caggctgacc ccgctctcgc ccatggtcac ctgcaccagc agctcgggct
     5101 tgcccacgat ggaagccagg ttcctcatcc tggcgtcgct ggaagacgcc gcaaggtcca
     5161 gcatggcagc cagcgacaga ccgggggaga cctgcagaga cgctccggcg gcgatgggct
     5221 tgacgctccg tccgccgacc agacgcccga caccgggcga cgtggccgaa ggcgatggcg
     5281 cgggcagcgg cggtaggacg ggaagcgtgt agttcttgaa cgtggtggaa gacagcagga
     5341 tcgcaaactg ctggaaggtg agaaagtctg ccaccttcag gtcgctgccg atgaccggca
     5401 gagacgagag cttggccttg ggtgggaagt cgatgccaaa cacgaatccc cacgtcccgg
     5461 tctgtccttt ccctgcggtg aagaacgcat ctccatgatg gcttgattca gccgccaggg
     5521 tgaagaaatt gtcggcggcg tggtattcga acgatgcggc ctgcagcccc agatcgagcc
     5581 catcgggcac ggagatggtg tgttcgatgc ccagtgcctc ggccagatcg ttgaacccga
     5641 tggtgcttcc atcgctggtg cgccagcggc cgatgaaggt cttcacgttg gcgccaagat
     5701 cataagacac gaggaacaga gccttgccga tctgcaggtc cgcttccatg tgcccggtga
     5761 acactcgctg gccggtggag ggatccaggg tggaagtcag gaaaacgcgc gtgtccagct
     5821 cgtaggatcg cggcccgagc ggaatctggc cggtggtggt cgtatctgcc gtgaactgaa
     5881 acgacttctg gtgcgtatcg aagaccaccg tgagctgatg gatctccagg ctctgcacgg
     5941 cctgcaggag cgcgggggga actgaagcgc cgatcttctg ggccacgtcc tggaggaacg
     6001 tgcccacggg aacgccgtgc tcggtcgcac ccaggccggt gaacagccag ccgaggccct
     6061 tgcccagata ctgcgcgttg aacacgatct cgaagccacc caggttcgct ccgacggtga
     6121 ttccaccgga caccgattgc ggcgatcgct ccaggttgac gcccaggttc tccagcgcga
     6181 gaaagggccc cgtgcctcgt cccgtcgggt cccacttcca gtcctcttcg gtcaggacct
     6241 cgatgcgata cgtgccctgg ccggggtagg cgaacaagca cagctgcgtg atggcgagct
     6301 ccggcaggtc ggcggcttcg ccgaaaacat gcgtggccag cgcctggacc gagatcctgg
     6361 agggcgccgc cggctgtccc ttgatggcag gtggcagtcc accgtagaaa ctgaagtccg
     6421 gcagctgggt atagagaaag atctggcctc cggccagcgt cagatggctc tgcagcgtga
     6481 cgtagagcag cttccggtgg accaggtcga agccgacccg gatgcggtcg atggagatgt
     6541 ctcccagccc gagggccttc acgccatcct ggacggcgga cttcaacgcc tcaccgccga
     6601 ggtggccggc gagctcggcg agacccggga gcacctgtgt gctgctcggc agcacgtcga
     6661 attccagccg gttgcgcccc agcagataca ggaggatctg gaagtccgac gaggcgatcc
     6721 gggtcgtggc atgcacgttt ccggcgagcg agaggtcggc gccgcctgcc ttggcaggtc
     6781 gatgcacagc gagcacggag acgcccacgc ccttgagggc aaacagcccg ccgtcgatga
     6841 tgtcccagtc gccgttcgcg tccgccgaga tctcgaagta ggtcgaggag ccgggagcga
     6901 agccgaagct cagctgcacg cccgaaagct ggatctgatc gaacagcgag atcccctggg
     6961 gggccaggct gctgctcgat cccgagagga aggcgagcgc atcgtggagg gagaacacct
     7021 gcttgtgcgg accggtcggc aacagggtga gcgtcaacag cagggtgccg ggcagggtag
     7081 atccgctgac ggagatcggc ttgccggcca gcgccagggt ggcctggctg atggcaagcg
     7141 tgctcaggat cggccccgaa gcgctcgtct gcaggaacga gatcttgacc tggatgctcg
     7201 agagcaagcc accgttgacg aacggcaccg tccagcttcc ccggacctgt ccggcgacat
     7261 ccagccgcgt gatggccggt gagccggtgg gcgtgacctt ggggtcttcc agcaccagtt
     7321 gctgctgggg cagggtccgc aggaacgcca ggaagtcgcc gtcggggatc atctccggct
     7381 tgagcgtcag cctgccatcg acggggtggg aagcttccag ccgggacttc aggagctcca
     7441 ggttcttgat atctgtcatg gctgtcgatc ctcgaaactc ggcgcggcgc gctggctaga
     7501 acggcatctg gaatgctccg aacggtgcgt aggcaaacgc agggttcttg tggatgttgg
     7561 tgccggcgtt cggactgaag aagtcgaccg tgaactgcac cagcgctgcg cccgcctgtg
     7621 cggcggtcag ccccaggcag aggtctcccc ggtgggtgtg cgcggcggtc cccggaagcc
     7681 ataccccggc cacggtcgcg aagttggtga ggttggccgt gccgttcaac cactctccgg
     7741 tcaggaaata ttgtctaacc gttggatcgt tgtggagcgc cgtgaccacc tgctgatcgg
     7801 ggtggtcata ctgattgtcg gtgccgcagc tgatgaacgc cgcccggggt cgcaggcggg
     7861 ccaggaacgc gggggacgac gagtatttac tgccatgatg gctggttttc agggcatgta
     7921 cccggttggc tacgttattg ccctggttga gatattgcat caggctgttg ttgcggttgg
     7981 cttgcgctac ggtgtagccg ttgccattcc actggctgcc atcctcctgc acggcgacga
     8041 tgtcaccacc gacgtaatac ttgaaattgc cgaatctgag caggaaggcc aggctcttgg
     8101 cattcttcaa ctgatcgccc agcacggccg ccgaaaccag ccgcgtcccg gccccggcgg
     8161 cgcgcaggac gtattgattg gcagccacgc aggtcagcgt aggagcgccc ggccctcggg
     8221 gcacgggggc tggtggcaag aggatctcgg tgcccaccag ccagtccgga tcatgccagc
     8281 cggcatggct gacaccgggg cccgatcgac cggcaaggac gcggccggtc ggacgcaccc
     8341 gggcgccgac ggcgttgaca taggcctgat actgtgggtc gcgtccactg acgtaccatc
     8401 tccagttttg agcccggtcc ctcttggccg tgaccatgcc cggctcgccc tgatcgtata
     8461 cacgtgtatt ggcatagatg gcattgccca tgttgagcag gctgcgcagg ccgccataat
     8521 gatcttcgtc atagtgcgtc gccaccatga catcgagcgc tgcgagtccc tgcgtggtga
     8581 tacggttgtg gacgtggttc gcgtacgagg tccggccgcc atcgatcaat gcggaacgcg
     8641 tcatggcaac gtgtcccgcg ccgacaccat cgtcggttgc gatgatgagg gtggaatctc
     8701 ccgaacccac gacgtcgatg tgccagatct ccaagctcca tgtcatggcg attctcctcg
     8761 atgtgcggaa cgagcgaatg cgggacgacg tgcgcagtgg ccggcctggc cggtgaagag
     8821 gcagctaccg ggagtccccg agagcccgga cgtcacaacc gcagcatggt accgcaagcc
     8881 cgggttccgg atcgccctca cgagctgcca cgggactcgc cgggatggac ccggccgcta
     8941 cttgcagctg accgacagca ggtggccttc gttgccgggc agcgagtgga cgtacatgag
     9001 gtagtcgccg gcctcgacgg tcgaggtgct gttggcgacc tccttgcccc gggcgtcgtt
     9061 catcgtcacg gtcagccgga ccttggactt gtcgacgatc tcgaccagcg tcgcggtcgc
     9121 cgagccgatc ttgagcggga tcggctcctg cttcttcttg gcgagcgcct tctgggtacg
     9181 gccgagcagc ttgaactggt tccaggtcga gaacggcggc ttcttgagct tcttctcgag
     9241 cggcttgagc tcgccgtcga ccgccggctt gtcgccggcc ctggccgaga tctcgaggaa
     9301 gtcgcagtcg gcggtgacgt cggcgcgggc ggcgccgggc gcggccaggg cgagcgcgag
     9361 cccggcgatc aggaggcggt ggccggcggg tcccgtcata acgtctccgc gtcatcgtcc
     9421 tggggggtca cccagatcac cgtggcgttg ccgtcgtcgt cgttgaggct gatcacggtg
     9481 ctggagccgc cgggagtgtc gagcgagtcg atctccgggg ccgcgcgctg cgccaccggc
     9541 cggacctcga tcgcgtgggc gccggaggcg ccgagatcgg agttgccggg gcgcaggacg
     9601 atggcgagcg cggcgaccgc tcccgcgctg accacgccgg tgatgacgtg gccgcggtgg
     9661 cggtcgaacc agccggagat ccgcgcccac aggccggtcg gcgcgacgag ctgggcgtcg
     9721 atccggcgcc acatcgcggc gaacctggcg tcgtggatcg tgtccgccga cagctccagg
     9781 tggccgcgca ccaggtcgcc gagctcctgg atcgcttcga gccgcacgcg ggcgtcgggg
     9841 tcgcgctcga gccgggcgat cagctccggg tcgtcgagct cgccgtccgc gtgctccatg
     9901 agctcgaggt cggacgggct ggtcggcggg cgggtcatgg cccgggtcat ggggtgccgc
     9961 tccgcgccgc gcgggcgagc gggtgagcgg gcgcgctggt ggccggcgca tcttcgggag
    10021 cggcgggagc ggcgccaccg gtcaggtcct cgaccagctc cgccgggggg tgatcgagca
    10081 ggtcgagcag ccgcttctgc atgttgcggc gggcgtggaa caaccgcgac atgatggtcc
    10141 ccttggagca gctcatcgcc tgcgccatgt cttcatacga cagtccttcg agctcgcgca
    10201 tcaccaggac cgcgcgatga ttctcggaca gctgatcgag cgcgagatcg atccgggcgc
    10261 ggatctgctt gtcgatcagc gcctggccgg gatttccgcc aagaatccgc ggcagtagcg
    10321 gatcgtcgcc gtcggcctcg aggcgggtct cgtcgagctc caccggcttg accttgcggt
    10381 gcttgcggag gtgatcgatg gccaggttca tgacgatccg gtagagccag gtgtagaagc
    10441 tcgcgttgcc ttcgaacttg tctaggtact tgtgagcctt gatgaaagcg tcctggacca
    10501 cgtccagggc gtcgtctgga tgacggacca cgcccagcgc cagcgcgtag gcccggcggt
    10561 ggtagcgctc gaacagcgtc ttgaacgcgt cgcgatcgcc gcgctgcgcg gcggcgacca
    10621 gctcgcggtc atcgaccggg tcacgtccat gcgtcgagat ggacgctcgc tgaccgggct
    10681 tattcacgct tgcccgtcat atcacggacc tcacgcgacc tgcgcggcaa acgcgtccgg
    10741 gccgcggcac tggctcgtcg gtgcgctatg ctgaaagccg ccgcgcggta aggcgacatc
    10801 gtggctgtcc ctcgcactcc gatcccgacg tttttgcagc gtgttcgcac gctcgtgtac
    10861 cggcacgagg cccggcgggt ggggctctat gcaacggcgg cggtcgccgg catcgccctg
    10921 gtgctgccgc tcgtcggcgt cgtggtctcg gcgagccgtg ccaccgcgct cgcggtgctg
    10981 gcgctcggcg ggctggtcgt cgtgctgctc gcggtcaccg cggtcgtgct cgggctgatc
    11041 gccccgcgcc ggcgctgggg cggcgacccg gcgctcgccc gctgggtggg cgtccggcgg
    11101 cgc
//
LOCUS       DASVOR010001119         3017 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_157284,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001119 DASVOR010000000
VERSION     DASVOR010001119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157284"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VF516_21395"
     CDS             complement(<1..1067)
                     /locus_tag="VF516_21395"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110307.1"
                     /translation="MVEDSGSERLTDVAEAATGPDDDPVGMRIGPGTEVGGYVIEGEL
                     GHGGMGVVYSATHPVIGKRAAIKILRLDVSRSPISVERFIQEARAVNQIGHPNIVDIF
                     AYGALPDGRAYHVMDLLVGESLRRRLRGGALHPSEAASVIDEIASALQAAHDHGFIHR
                     DLKPDNVFLVQRENRWPEVKLLDFGLAKLMPERGVPAFTTKTGVMLGTPEYMSPEQAR
                     GTGVDHRTDIYALGVVMFEILAGHRPFPALADSLATLMQHAEEPPASLGDTVAGLPAE
                     MVQLVDTMLAKTPAARPSLAAVRTVIKRLRTTQLPTRSVAGMEVLSYQPTAPPLAITP
                     SKVGAEAMLPGDSLLSDRARPP"
     gene            complement(1077..2393)
                     /locus_tag="VF516_21400"
     CDS             complement(1077..2393)
                     /locus_tag="VF516_21400"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8110308.1"
                     /translation="MNRLALAVAMLAACGDDINNGGGDETRQGGDLTVDDRTAFAFTH
                     PASGLTADQQNAFALGRSAFLFHWPPPVLGPLFNADGCGDCHAGNGRGLSQIGNGALQ
                     SQALIRVSLVDGAPGAMPQVPGGDAPLAGIGLQLHDHASGALPEAVVSQSWVEHAETY
                     GDGTVVMLHEPSLVVTRVDGSSFGPDVRMSYRQSLGMFGLGLLEAVSEATLRALADPD
                     DADRDGISGRVNLVWDLEQQTTGVGRFGHKATVPTLRQQVALAFAGDIGLTNKLLPAA
                     LGDNDLADSQFDETVLFVSVLGVPAAAPQNAAASAGRQLFRDFGCAACHVPTLVTGDA
                     ALSQLAHQIIHPYTDLLLHDVGDRLTDARRDFTAEGVEWRTAPLWGIGLVQVVQPAAT
                     FLHDGRARTLAEAILWHGGEAQAARDAFRLAAKPERDALISFLQTL"
     gene            complement(2484..>3017)
                     /locus_tag="VF516_21405"
     CDS             complement(2484..>3017)
                     /locus_tag="VF516_21405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEX8110309.1"
                     /translation="DYGTGDAPPPTPAEYGAAVRAALAPLTALGVTLYTEPGRVIVGR
                     AGALVTRVLYRKRNEAKHFTIVDGAMNDLMRPALYGSFHPMKPVARPDRPVIATDIVG
                     PICETGDFFARDRELPELAPGELLWVGAAGAYGSAMASNYNTRPRAPEVLVAGDRYTV
                     IRDRETYDQLLAGERLA"
BASE COUNT          402 a         1079 c         1131 g          405 t
ORIGIN      
        1 ggcgggcgcg cgcggtcgga cagcagcgag tcgcccggca gcatggcctc ggcgccgacc
       61 ttcgacggcg tgatcgcgag cggcggcgcg gtcggctggt acgacagcac ctccatgccg
      121 gcgaccgagc gggtcggcag ctgggtcgtg cgcagccgct tgatcacggt gcgcaccgcg
      181 gcaagcgacg gccgcgccgc gggcgtcttg gccagcatcg tgtcgaccag ctgcaccatc
      241 tcggccggca gcccggcgac cgtatcgccg agcgacgcgg gcggctcctc ggcgtgctgc
      301 atcagggtcg ccagcgagtc ggcgagggca gggaacggcc ggtgcccggc gaggatctcg
      361 aacatcacga cgccgagcgc gtagatgtcg gtgcggtgat cgacgccggt gccgcgcgct
      421 tgctcgggcg acatgtactc cggcgtgccg agcatcacgc cggtcttggt cgtgaacgcc
      481 gggacgccgc gctccggcat cagcttggcg agcccgaagt cgagcagctt gacctccggc
      541 cagcggttct cgcgctggac caggaacacg ttgtccggct tgaggtcgcg gtggatgaag
      601 ccgtggtcgt gcgcggcctg cagcgccgag gcgatctcgt cgatcaccga cgccgcctcg
      661 ctggggtgca gcgcgccgcc ccgcagccgg cggcgcagcg actcgccgac caggaggtcc
      721 atgacgtggt aggcgcggcc gtcgggcagc gcgccgtacg cgaagatgtc gacgatgttg
      781 gggtgtccga tctggttgac cgcgcgcgcc tcctggatga accgctcgac cgagatcggc
      841 gagcgcgaca cgtcgagccg caggatcttg atcgccgcgc gcttgccgat caccgggtgg
      901 gtcgcgctgt acaccacgcc catgccgccg tggccgagct cgccctcgat gacgtagccg
      961 ccgacctcgg tgccgggacc gatgcgcatg ccgaccggat cgtcgtcggg gccggtcgcg
     1021 gcctcggcca cgtcggtcag acgctcgctg cccgagtcct ctaccacccg gcgattctac
     1081 agtgtctgca gaaaactgat cagggcgtcg cgctcgggct tggcggcgag ccggaacgcg
     1141 tcccgcgccg cctgggcctc gccgccgtgc cacaggatgg cctcggccag tgtgcgggcg
     1201 cgcccatcgt gcaggaaggt ggcggcgggc tgtaccacct ggaccagccc gatcccccac
     1261 agcggcgcgg tccgccactc gacgccctcg gcggtgaagt cgcgccgcgc gtcggtcagc
     1321 cggtccccga catcgtggag cagcagatcg gtgtagggat ggatgatctg gtgcgcgagc
     1381 tgggagagcg ccgcgtcgcc ggtgaccagc gtcggcacgt ggcacgcagc acagccgaag
     1441 tcgcggaaca gctggcggcc cgcgctggct gccgcatttt gcggcgccgc ggccggcacg
     1501 ccgagcaccg acacgaacag cacggtctcg tcgaactggc tgtcggccag gtcgttgtcg
     1561 ccgagcgccg ccggcaggag cttgttggtc aggccgatgt cgccggcgaa tgcgagcgcg
     1621 acctgctggc gcagcgtcgg cacggtcgcc ttgtgaccga accggccgac ccccgtggtc
     1681 tgctgctcga ggtcccacac caggttgacc cgcccggaga tcccgtcgcg atcggcgtcg
     1741 tcggggtcgg cgagcgcccg gagcgtcgcc tcggaaaccg cctcgagcag gccgagcccg
     1801 aacatgccga gcgactggcg gtacgacatc cggacgtcgg ggccgaacga cgagccgtcg
     1861 acccgggtga ccaccaggct gggctcgtgc agcatcacga ccgtgccgtc gccgtaggtc
     1921 tcggcgtgct cgacccacga ctggctgacc accgcctcgg gcagcgcgcc cgacgcgtgg
     1981 tcgtggagct gcagcccgat cccggcgagc ggcgcgtcac ctcccgggac ctggggcatc
     2041 gcgccgggcg cgccgtcgac caggctgacc cggatcagcg cctgcgactg cagcgcgccg
     2101 ttgccgatct gcgacagccc gcggccgttg ccggcgtggc agtcgccgca gccgtcggcg
     2161 ttgaacagcg gaccgagcac cggcggcggc cagtggaaca ggaacgcgct gcgccccagc
     2221 gcgaacgcgt tctgctggtc ggcggtcagc cccgaggccg ggtgggtgaa cgcaaaggcg
     2281 gtgcggtcgt cgacggtgag atcgccgccc tggcgggtct cgtcgccgcc cccgttgttg
     2341 atgtcgtcgc cgcacgcggc cagcatcgcg acggcgagtg cgaggcggtt caccaccgcc
     2401 aaggctaccg gcccggcggg cgccaggcca gtgcagacgc cggagcggtg gatcgccccg
     2461 ccggtcgccg cggtcgccgc gggtcaggcc aggcgctcgc cggcgagcag ttggtcgtag
     2521 gtctcgcgat cgcggatcac cgtgtagcgg tcgcccgcga ccaggacctc cggggcgcgc
     2581 ggccgggtgt tgtagttcga cgccatcgcc gagccgtacg cgccggcggc gccgacccac
     2641 agcagctcac ccggcgcgag ctcgggcagc tcgcggtcgc gcgcgaagaa gtcgccggtc
     2701 tcgcagatcg gcccgacgat gtcggtcgcg atcaccgggc ggtcgggccg ggccaccggc
     2761 ttcatcgggt ggaacgagcc gtacagcgcc ggccgcatca ggtcgttcat cgccccgtcg
     2821 acgatcgtga agtgcttggc ctcgttccgc ttgcggtaca gcacgcgagt gaccagcgcg
     2881 ccggcgcggc cgacgatcac ccggccgggc tcggtgtaca gcgtcacgcc cagcgcggtc
     2941 agcggggcga gcgcggcgcg gaccgcggcg ccgtactcgg ccggcgtcgg cggcggtgcg
     3001 tcgcccgtgc cgtagtc
//
LOCUS       DASVOR010001120         2101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_390926,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001120 DASVOR010000000
VERSION     DASVOR010001120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_390926"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF516_21410"
     CDS             <1..369
                     /locus_tag="VF516_21410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110310.1"
                     /translation="GAADAAVTPGPTKDPHDHDAEVAASVFADSFESGHMHPPASTPA
                     VEPSAEAPAASSAPAAPEAKVTLPPTAGEPGEATASHADADLAALVKPAPNTIARGAT
                     TSTSAGTAPAAEKPPEPDHG"
     gene            362..1204
                     /gene="tatC"
                     /locus_tag="VF516_21415"
     CDS             362..1204
                     /gene="tatC"
                     /locus_tag="VF516_21415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEX8110311.1"
                     /translation="MAKADDEAAEQERLGREAELEEGRMPFLQHLGELRDRVRNAALY
                     FVGAFLVCWYFAEDLFDWLKQPLFHIWLQHTGPGIPKAEDWGPPQMISTGVTQPFWVY
                     MSVALWAGVFVASPFMFYQLWKFIAPGLYKRERKMGLSFGVLSGAFFIAGALFCFYFA
                     LPALFRYMLHYNSSDMKFLLEIEKYLDLVRDSMLAFGLVFEMPLLIYFLALVGLVTHR
                     GLWKFNRWFVVLAFIIGAVLTPGPDVVSQFLMATPMIVLYNISILFAWRVTVRRERAA
                     AGSR"
     gene            complement(1580..>2101)
                     /locus_tag="VF516_21420"
     CDS             complement(1580..>2101)
                     /locus_tag="VF516_21420"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8110312.1"
                     /translation="PSFGGSPGSVAPPAPTPTPARPSAPSLTDSPKDPYGDDAPAAGA
                     PPDRKAEFFANLGGQQLIGGDTAGAAASFQKALELDARNVTAMMGMGEIALRQGLFGD
                     AIAHLSKASKLVPKNVKVLTLLGEAYLSSGNNAQAAAQFKKALQLDPDNARARDGYNE
                     ASSRVPPPDDDAQ"
BASE COUNT          293 a          757 c          702 g          349 t
ORIGIN      
        1 ggcgccgccg acgccgcggt cacccctggc cccaccaagg acccgcacga ccacgatgcc
       61 gaggtcgcgg ccagcgtgtt cgccgactcg ttcgagagcg ggcacatgca cccacccgca
      121 tcgacgcccg ccgtcgagcc ctctgccgag gctccagcgg cttcctcggc tccggcggcg
      181 cccgaggcca aggtcaccct gccgccgacc gccggggagc ccggcgaggc gaccgcctcc
      241 cacgccgacg ccgacctggc cgcgctggtg aagccggcgc ccaacaccat cgcccgcggc
      301 gcgaccacga gcacgagcgc cgggacggca cccgcagcag agaagccgcc agagcccgac
      361 catggctaag gccgacgacg aagctgccga gcaggaacgg ctgggccgcg aggcggagct
      421 ggaggagggc cgcatgccat tcctccagca tctcggcgag ctgcgcgatc gcgtccgcaa
      481 cgccgcgctg tacttcgtcg gggcattcct tgtctgctgg tacttcgcgg aggacctctt
      541 cgactggctc aagcagccgc tgttccatat ctggttgcag cacaccggac cgggcattcc
      601 gaaggcggag gactgggggc cgccccagat gatctcgacc ggtgtcaccc agccgttctg
      661 ggtctacatg tccgtcgcgc tgtgggccgg ggtcttcgtc gcctcgccgt tcatgttcta
      721 ccagctgtgg aagttcatcg cgccggggct gtacaagcgc gagcgcaaga tgggcctgtc
      781 gttcggcgtg ctgtcggggg cgttcttcat cgccggagcg ctgttctgct tctacttcgc
      841 gctgccggcg ctgttccgct acatgctcca ctacaactcg tcggacatga agttcctgct
      901 cgagatcgag aagtatctcg atctggtgcg ggattcgatg ctcgcgttcg gcctcgtgtt
      961 cgagatgccg ctcctgatct acttcctcgc gctggtcggc ctggtcacgc accgcgggct
     1021 gtggaagttc aaccgctggt tcgtcgtgct ggcgttcatc atcggcgccg tgctcacgcc
     1081 ggggcccgac gtggtgtcgc agttcctgat ggcgacgccc atgatcgtcc tctacaacat
     1141 ctcgatcctg ttcgcgtggc gagtcaccgt ccgccgcgag cgcgccgccg ccggcagccg
     1201 gtagcgggcc gccatggcgc cggcccgatc gcgcaccgcc tcgcgtgacg ttcccatcgc
     1261 gccatcgaca cgatcccacg tccctgccgg caggagagat ctccccctca ccgagagcag
     1321 ttgcagggat cgatcgatac ccctaccgtg aggatatggt cctcggcaaa ccgcgtccca
     1381 agatctcttg gtcgagccgc agccttcgcc tcgggcgcct cggggtttcc caccacggca
     1441 caccgtcgag ctggaccccg ccgaccaagg accacgcgcc ggtgcacacg aagccgaaga
     1501 agcgaccgta gcccacactg cccgcaccta gcggactgcg gatcgaggac gtgtctggca
     1561 atcgcccgtc ctcgggcggc tattgcgcgt cgtcgtcggg cggcggcacg cggctcgacg
     1621 cctcgttgta gccgtcgcgg gcgcgcgcgt tgtcgggatc gagctgcagc gccttcttga
     1681 actgcgccgc ggcctgggcg ttgttgccgc tgctgaggta ggcctcgccg agcagggtca
     1741 gcaccttgac gttcttgggc acgagcttcg acgccttcga caggtgcgcg atcgcgtcgc
     1801 cgaacagccc ctggcgcagg gcgatctcgc ccatgcccat catcgccgtg acgttcctgg
     1861 catcgagctc gagcgccttc tggaagctcg cagcggcgcc cgcggtatcg ccgccgatca
     1921 gctgctggcc gccgaggttg gcgaagaact cggccttgcg atcgggcggc gcgccagcgg
     1981 ccggcgcatc gtcgccgtac ggatccttgg gactgtcggt gaggctcggc gcggacggcc
     2041 tggcgggcgt gggcgtgggc gccggcggcg cgacgctgcc cgggctgccg ccgaagctcg
     2101 g
//
LOCUS       DASVOR010001121         3733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_157300,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001121 DASVOR010000000
VERSION     DASVOR010001121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157300"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            470..2287
                     /locus_tag="VF516_21425"
     CDS             470..2287
                     /locus_tag="VF516_21425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110313.1"
                     /translation="MPTTQTTRLARLIFSVGLLMTGCGTPPGDPQSAATAQAVTGSCS
                     SPIAIHGNGDQPQATTAGQPSSTDIDNSLSHNANDWTTDIGCSDVVYQLTGAGTTVTL
                     TLRPAGSDWNGVLYVRSASQGCTTDPYAPVTDASERSAGANESISFLAQSGVVYYVSV
                     AGYGGADGVACASGSFVLNVAGLTGTGGSGTCASPIAITGGGDQPQATTVGKPSNTDV
                     DNSLSHNASDWTTDIGCSDVVYRLTGTGSTVTLTTRPVGSDWNAVLYARSASQGCATD
                     PYTAITDGSERTAGASESISFVAQAGVDYYVSVGGYGVASSGTCSAGSFVLHMSNGVT
                     GGPPLSYGQWAYGAPPVDGIVRAGFTWFNTGYPGTVSATDNTTLRNASVLPIAYINSS
                     GVPQDDTSLQGQINQYFPGSSWNAIDLHCPDAGWNVARVDVRDSRWQDWLIARANAAY
                     SLGNRGIFWDEAAVENLCYGDPAQQQQAVDALGSVMTRLRAQHPDLQFIVNHGFALAE
                     QYTSLVSGFMQENLLNYYEQTGHDPWSTDEIAHTNRVRDLGIKILDMEYIDLMGCDDT
                     TCNYATQLINESAGMSWVPYVTRQLMNYEGRGWTITPPW"
     gene            complement(2351..2884)
                     /locus_tag="VF516_21430"
     CDS             complement(2351..2884)
                     /locus_tag="VF516_21430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110314.1"
                     /translation="MSNRITDAGAIEARLLELAHTTEAKITAPALAYFAPCSLDDAGR
                     VLDDLAARDRLSMEIEDDGTVVYQMPGRQKLAAPPPSPTTPPLSPALVRMAQSSRGPS
                     PLLAALLSALIPGAGHLYAERPVAAILWFLVVGLGYTLLLPGLVLHMFSIASAASSAR
                     RLEAVRARPLLPSFTVR"
     gene            complement(2980..3390)
                     /locus_tag="VF516_21435"
     CDS             complement(2980..3390)
                     /locus_tag="VF516_21435"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8110315.1"
                     /translation="MNTIDAQTVRAGEFPNTVRVRSHSFTSDVGASTGGQDSGPGPHD
                     LFDAALATCKTMTAMWYARRHGIPLERVEAHVESDDSQERAGVYRMRVRVELFGPMTD
                     EQRAQVHRAIAACPIHKLMTTSDVQIEVVPPAAG"
     gene            complement(3408..>3733)
                     /locus_tag="VF516_21440"
     CDS             complement(3408..>3733)
                     /locus_tag="VF516_21440"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEX8110316.1"
                     /translation="ALDLPGGAAERAVFVIEGELRIAEHTLTADRLAVLCGDRPVHAV
                     ASAPTRALILGGPPLGHRFIEWNFVSSSRDKIDRARDLWKAQQFPRIPTDDVEFVPWP
                     ELHQS"
BASE COUNT          600 a         1286 c         1316 g          531 t
ORIGIN      
        1 tgttcgatca gttcctcgaa gattcggact tcgcccggcg ctgtcgctag agaggcatgg
       61 ggctggcgaa tccacgccgc gcttgccatc cccgaagcag ggcctcgagc ccgaccccag
      121 aaaacacctg acctggctcg ccgcgttgcc cggatgcagc gctctcttcg ctgccgatcg
      181 tttcatgaca ctcgggtagc cctccgccgc ggcgtctcga cctccccaaa tccgctttga
      241 gatccggggt atctcgggtc cgggccagga ggccagcgcg gctgtgtcga gccgccgagc
      301 cactgcggcg ttgccgacgc cgtttgtgaa gccgcgtgat gctgcatcgc aagcgcccgt
      361 gcagggccgc gcgctgtgcg tgaggtgctc gcacccgatc gcaggatttg cgatccgcag
      421 ccacgcgcgt ggcgcgcgcg atgcacgcgg ggatgcaatg aggacctgta tgcccacgac
      481 acagacaacc aggctcgccc ggctcatatt cagcgttggc ctgctgatga ccggctgcgg
      541 cacgccgccg ggcgatccgc agtcggccgc gaccgcacag gcggtgaccg gcagctgcag
      601 ctcgccgatc gcgatccacg gcaatggcga tcagccccag gccaccacgg ccggtcagcc
      661 gagcagcacc gacatcgaca actcgctatc gcacaatgcc aatgactgga cgaccgacat
      721 cggctgctcg gacgtcgtct accagctcac cggcgccggc accacggtga cgctgacact
      781 gcgtccggcc ggcagcgact ggaacggcgt gctgtacgtg cgcagcgcga gccagggctg
      841 caccaccgac ccgtatgcgc cggtcaccga cgcctcggaa cgcagcgccg gcgcaaacga
      901 gtcgatcagc ttcctcgccc agtccggcgt ggtctactac gtctctgtcg ccggctacgg
      961 cggcgccgac ggcgtcgcgt gcgcctcggg gagcttcgtg ctcaacgtcg cgggcctgac
     1021 gggcaccggc ggcagcggca cgtgcgcgtc gccgatcgcg atcaccggcg gcggcgacca
     1081 gccgcaggcg acgacggtcg gcaagccgag caacaccgac gtcgacaact cgctgtcgca
     1141 caatgccagt gactggacga ccgacatcgg ctgctccgac gtggtgtacc ggctgaccgg
     1201 gaccggctcg acggtaaccc tgaccacgcg cccggtcggc agcgactgga acgcggtgct
     1261 ctacgcgcgc agcgcgagcc agggctgcgc gaccgatccc tacaccgcga tcaccgacgg
     1321 ctcggagcgc accgcgggag ccagcgagtc gatcagcttc gtcgcccagg ccggcgtcga
     1381 ttactacgtc tcggtcggcg gctatggcgt cgcgagcagc ggcacgtgca gcgccggcag
     1441 cttcgtcctc cacatgtcga acggcgtgac gggcggcccg ccgctatcgt acggccagtg
     1501 ggcctacggt gcgccgccgg tcgacggtat cgtgcgcgcc gggttcacct ggttcaacac
     1561 gggatatccc ggcacggtgt cggcaaccga caacacgacg ctgcgcaacg ccagcgtgct
     1621 gccgatcgcg tacatcaact cgtccggcgt gccgcaggac gacacctcgc tgcagggcca
     1681 gatcaaccag tacttcccgg gctcgtcgtg gaacgcgatc gatctgcact gcccggacgc
     1741 gggctggaac gtcgcccggg tcgacgtccg cgattcgcgc tggcaggact ggctgatcgc
     1801 gcgcgccaac gccgcctact cgctcggcaa ccgcggcatc ttctgggacg aggcggcggt
     1861 cgagaacctg tgctacggcg acccggcgca gcagcagcag gcggtcgacg cgctcggcag
     1921 cgtcatgacc cggctgcgcg cgcagcaccc ggatcttcag ttcatcgtga accacggctt
     1981 cgcgctggcc gagcagtaca catcgctggt gtccggcttc atgcaggaga acctgctcaa
     2041 ctactacgag cagaccgggc acgacccgtg gtcgaccgac gagatcgcgc acaccaaccg
     2101 cgtccgcgat ctcggcatca agatcctcga catggagtac atcgacctga tgggctgtga
     2161 cgacaccacg tgtaactacg cgacacagct gatcaacgaa tccgcgggga tgagctgggt
     2221 accgtatgtc actcgccagc tcatgaacta cgagggccgc ggatggacga tcaccccgcc
     2281 gtggtgaggt tctcgcgaga cccgagccag agctgatctc cgcgccgggc agggagagcg
     2341 cggccgcggt tcaccgcacg gtgaacgacg ggagcagggg ccgggcgcgc acagcctcga
     2401 gccggcgcgc ggacgaagca gccgacgcga tgctgaacat gtggaggacc aggccgggga
     2461 gcagcagggt gtagccgagg ccgacgacca ggaaccacag gatcgcagcg accggtcgct
     2521 cggcgtagag gtggccggcg cccgggatca gggcgctcag cagggcggcg agcaacgggc
     2581 tcgggccacg cgaggactgc gccatcctga ccagcgccgg cgacaacgga ggcgtggttg
     2641 gcgagggcgg cggggccgcg agcttctggc ggccgggcat ctggtagacg acggtgccgt
     2701 cgtcctcgat ctccatgctc agccgatcgc gggccgcgag atcgtcgagc acgcggccgg
     2761 catcgtcgag cgagcacggc gcgaagtacg ccagcgccgg cgcggtgatc ttcgcttccg
     2821 tggtatgcgc gagctccagg agccgcgcct cgatggcgcc cgcgtcggtg atccggttgg
     2881 acacggtgtg tatgtaaggc atcggtgacg agttgccacc ggccatccgg gtaggtcggg
     2941 cgcccgagat ccgcgctcgg agaacctcgc agcgcgtgac tacccggcgg ccggcggcac
     3001 gacctcgatc tggacgtccg aggtggtcat cagcttgtgg atcgggcacg cggcgatcgc
     3061 gcggtggacc tgggcgcgct gctcgtcggt catcggcccg aacagctcga cccgcacccg
     3121 catgcggtag acgccggcgc gctcctggct gtcgtcgctc tcgacgtgcg cctcgacgcg
     3181 ctcgagcggg atgccgtggc gcctggcgta ccacatcgcg gtcatggtct tgcacgtcgc
     3241 gagcgcggcg tcgaacaaat cgtgggggcc cgggccggag tcctggccgc cggtggaggc
     3301 gccgacgtcc gacgtgaacg agtggctgcg gacgcggacg gtgttcggga actcgcccgc
     3361 gcggacggtc tgggcatcga tcgtgttcat gggtcacctc gagaggttca gctctggtgg
     3421 agctcgggcc aggggacgaa ctcgacgtcg tcggtcggga tcctggggaa ctgctgggcc
     3481 ttccacaggt cgcgcgcacg gtcgatcttg tcgcgcgacg acgacacgaa gttccactcg
     3541 atgaagcggt ggccgagcgg ggggccgccg aggatcagcg cgcgggtcgg cgcgctcgcc
     3601 acggcgtgca ccggccggtc gccgcacagc accgcgagcc ggtcggcggt gagcgtgtgc
     3661 tcggcgatgc gcagctcgcc ctcgatcacg aacaccgcgc gctcggcggc gccgccgggg
     3721 aggtcgagcg cgg
//
LOCUS       DASVOR010001122         3066 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_186995,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001122 DASVOR010000000
VERSION     DASVOR010001122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186995"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..629
                     /gene="rpmH"
                     /locus_tag="VF516_21445"
     CDS             480..629
                     /gene="rpmH"
                     /locus_tag="VF516_21445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008589899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEX8110317.1"
                     /translation="MKRTYQPHNKSRKRTHGFRARKKTRGGREVLRRRRAKGRKRISV
                     TIAKK"
     gene            642..1010
                     /gene="rnpA"
                     /locus_tag="VF516_21450"
     CDS             642..1010
                     /gene="rnpA"
                     /locus_tag="VF516_21450"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEX8110318.1"
                     /translation="MPRRASSGISKQMRLRRRAEFLAVQAGGTKLHGRHVLAIAQRRG
                     DDAAGGDARGRLGLTVTKKVGNAVVRNRIKRLLREWMRLHGWVPAGWDLVLVAKDSAA
                     RQLHPDDFAIDLTRILRQLS"
     gene            1043..1273
                     /gene="yidD"
                     /locus_tag="VF516_21455"
     CDS             1043..1273
                     /gene="yidD"
                     /locus_tag="VF516_21455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012865047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEX8110319.1"
                     /translation="MLLPVRVYRVALSPLKRRPTCRYLPTCSEYAITAVEQRGIVVGL
                     GLAAWRILRCNPLGAGGYDPVPPRRARHCQEH"
     gene            1273..>3066
                     /gene="yidC"
                     /locus_tag="VF516_21460"
     CDS             1273..>3066
                     /gene="yidC"
                     /locus_tag="VF516_21460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEX8110320.1"
                     /translation="MQDQGKRLLLAVALALGVFLVWSMFAKQDEPAKPAQTASQTTGS
                     GPAPAPGQLPAVPQIGPPDGSPGAPPAPAEGATISLPFDRFVATFSSACGGLTSWKLT
                     DERYQRDTTRGELLPARSQMTADGKPVPPAQLANLPACGAFDVNFVTATSSYFVPRSA
                     VWKGEKVSPTEVRYSYGSPSEPLEIVKDFKVFPQDYLVRMTVKVVSHAADGREAHEQL
                     AVTAYEYQDPAELKNGSSRVPARAWSSATLRPDDTLIDTDVSAVIEWPRFEPAVRWAG
                     FLHPYLLAGYAPEDNGPVAKHTSAADGTKGLPRGFMRTDLVFPGAMLRPGESLSRTVV
                     AYLGPKNYDDLRAADTTAGFQTGFDKTVDLGWFAFLGRPLLWLLQKFQSVVGNWGIAI
                     ILLTIVVKLLTLYWTTQSMRSMKETAALAPQLKALQAKYADDKQRQQAETMALYKEHG
                     VNPVAGCLPMLLQMPIWIALYRMLYSAGELYLQPFIPGWINDLTATDPYYILPGVLFI
                     SMFLQARLQPASVDSTQQKFLQYGMPIMFGGMALFFPSGLTLYMFTNNVLSALHSIYM
                     NKFDRRSLAAAAKLKKNQELATQTAAAAAKPG"
BASE COUNT          519 a         1034 c         1028 g          485 t
ORIGIN      
        1 cgagaaagtt cgactcgaac cacgaccgga tgtagctgtt gggagcgcgg aggcgcaacg
       61 tcgcgccatc aaacgacaat agctcgatcg gacgtagcca catgtcgtag aattgcggag
      121 atatccgcga ccggagtctc cccacggctt cgtcccaaag gagcggtact gtcattcccc
      181 ttttcgtcct gtgaaatctt gtggttggct gagttagcgc acagacaaaa gacaaccctt
      241 ccagaagtta tccacaggga cttctggtgc ccacccgcgg ccgtgaccaa aggccctccc
      301 acaggcgagg aaacctcgcg ttggtagcag acggggaatt cacgttgcaa gatcaaaacg
      361 atcggaagtg aactccggac ctcgtcggaa acgaggctgc caccgaggga acaacgctgg
      421 cttgacttca cccggttgct tgagtatcga tcccgctccc cgcagggtgg agtcaagccg
      481 tgaagcgtac ctaccagcca cacaacaaga gccgcaagcg gacccacggg ttccgtgcac
      541 gcaagaaaac ccgcggtgga cgtgaggttc tccgtcgtcg ccgagccaag ggccgcaagc
      601 gcatctcggt caccatcgcc aagaagtagc gtgagcgcct cgtgccgcgc cgcgcgtcgt
      661 cgggcatctc caagcagatg cggctgcggc gccgcgccga gttcctcgcg gtgcaggcgg
      721 gcggtacaaa gctgcatggc cgtcatgtcc tggcgatcgc gcagcggcga ggcgatgatg
      781 ccgccggtgg tgacgcgcgc gggcggctcg gcttgacggt caccaagaaa gtggggaacg
      841 cggtcgttcg aaaccggatc aagcgcttgc tgcgcgaatg gatgcggctg cacggatggg
      901 tccccgccgg ttgggacctg gtcctggttg ccaaggacag cgcggcgcgg cagcttcatc
      961 cagacgactt cgcgattgat ctcactcgga tcctgcgcca gctctcatga ctcgctcggt
     1021 ctactcagcg ctcgtatggc tcgtgctcct gccggtgcgc gtgtaccgcg tggcgctgtc
     1081 gccgctcaag cgcaggccca cctgccgcta cctgccgacg tgctccgagt acgcgatcac
     1141 ggccgtggag cagcgcggga tcgtcgtcgg actggggctc gccgcctggc gcattctgcg
     1201 ctgcaacccg ctgggagccg gcggatatga tccggtgccg ccgcgccgtg cgcgccactg
     1261 tcaggagcat tgatgcagga ccaaggtaag cgactgttgc tcgcggtggc gctcgcgctg
     1321 ggcgtgttcc tggtgtggag catgttcgcc aagcaggacg agccggccaa gccggcgcag
     1381 acggcgagcc agaccaccgg atcgggaccg gccccggccc cgggccagct cccggccgtg
     1441 ccgcagatcg gcccgccgga cggtagcccc ggggcgccac cggctcccgc cgagggcgcg
     1501 acgatctcgc tgccgttcga ccgcttcgtc gcgacgttct ccagcgcgtg cggcgggctg
     1561 acgtcgtgga agctgaccga cgagcgctac cagcgcgaca cgacgcgcgg cgagctgttg
     1621 ccggcgcggt cgcagatgac ggccgacggc aagccggtgc cgccggcgca gctcgcgaac
     1681 ctgccggcgt gcggcgcgtt cgacgtcaac ttcgtcaccg ccacgtcgag ctacttcgtg
     1741 ccgcggagcg cggtgtggaa gggcgagaag gtgtcgccga ccgaggtccg ctacagctac
     1801 ggctcgccgt cggagccgct cgagatcgtc aaggacttca aggtcttccc gcaggactac
     1861 ctggtgcgca tgacggtcaa ggtcgtctcg cacgccgcgg atggccgcga ggcgcacgag
     1921 cagctcgcgg tcaccgccta cgagtaccag gacccggccg agctcaagaa cggctcgtcg
     1981 cgggtcccgg cgcgggcgtg gtcgtcggcg acgctgcggc ccgacgacac gctgatcgac
     2041 accgacgtgt ccgccgtgat cgagtggccg cggttcgagc ccgcggtccg gtgggccggc
     2101 ttcttgcatc cgtacctcct ggccggctat gcgcccgagg acaacgggcc ggtcgccaag
     2161 cacacgtcgg cggccgacgg gaccaagggc ctgccgcgcg gcttcatgcg caccgacctg
     2221 gtgttccccg gcgcgatgct ccggcccggc gagtcgctgt ctcggaccgt ggtcgcgtac
     2281 ctcggcccca agaactacga cgaccttcgc gccgccgaca ccacggcggg gttccagacc
     2341 ggcttcgaca agacggtcga cctcgggtgg ttcgcgttcc tcggccggcc gctcctgtgg
     2401 ctgctccaga agttccagag cgtcgtcggc aactggggca tcgcgatcat cctgctcacg
     2461 atcgtggtca agctgctgac gctgtactgg acgacgcagt cgatgcggtc gatgaaggag
     2521 accgccgcgc tggcgcccca gctcaaggcg ctccaggcca agtacgccga cgacaagcag
     2581 cgccagcagg ccgagaccat ggcgctgtac aaggagcacg gggtcaaccc ggtcgcgggc
     2641 tgtctgccga tgctcctgca gatgccgatc tggatcgcgc tgtaccggat gctgtacagc
     2701 gccggcgagc tctacctcca gccgttcatc ccgggctgga tcaacgacct gaccgcgacc
     2761 gacccgtact acatcctgcc gggggtgctg ttcatctcga tgttcctcca ggcgcggctg
     2821 cagcccgcgt cggtggactc gacgcagcag aagttcctgc agtacggcat gccgatcatg
     2881 ttcggcggca tggcgctgtt cttcccgtcg gggctcacgc tctacatgtt caccaacaac
     2941 gtgctgagcg cgctgcactc gatctacatg aacaagttcg acaggcgcag cctggcggcg
     3001 gcggccaagc tcaagaagaa ccaggagctc gcgacgcaga ccgcggcggc ggccgccaag
     3061 ccgggt
//
LOCUS       DASVOR010001123         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_273717,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001123 DASVOR010000000
VERSION     DASVOR010001123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273717"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..542)
                     /locus_tag="VF516_21465"
     CDS             complement(81..542)
                     /locus_tag="VF516_21465"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEX8110321.1"
                     /translation="MSEHRATIEWAGGATAGDFLKLRFTREHRWTFDGGVEVLASASP
                     SVVPAPWSNARGVDPEEAYVAAISSCHMLTFLFVAAKAGFAVASYRDDAVGVMTKTPA
                     GVAWVSRVTLSPKITYAGDRQPSAEELARLHHAAHEGCFIANSVKTEIVVA"
     gene            645..>3094
                     /locus_tag="VF516_21470"
     CDS             645..>3094
                     /locus_tag="VF516_21470"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HEX8110322.1"
                     /translation="MIDRHDDTRPRDDAASLRRDLVGKLYFELAKFPEVATRNDQFLA
                     LAFAVRDRVLHRWVESSRTYLEGKSRSVVYLSAEFLIGPQLAANLLALGLHTAARTAL
                     ADLGISLDELVEHEEEPGLGNGGLGRLAACFMESMATLDIPAIGHSLRYEFGTFDQEI
                     RDGWQIEHTDRWLRNGNPWEVRRFEIEHPVGFGGFVEHTTDLHGRLRVYWHPERRVKG
                     IPYDVPVPGYGTNTTCFLRLWSAAADEEFNLDAFQVGEYWRAVDQKIQSENLTKVIYP
                     NDTSPAGKQLRLEQEYFFVSCALQDCIRLLLQRGTIREFADKFAIQLNDTHPSLAIPE
                     LMRLLIDVHDLGWNEAWDLVRRSIAYTNHTLLPEALETWPLPLIARLLPRHLEIIYEI
                     NRRFLDEVRAKFPGDEARLRRMSLIGEDGDKVVRMAHLATVASHHVNGVAALHSRLLR
                     QTVLRDFAELWPERFTSVTNGVTPRRFVALANPRLADLITEAIGDGWLRDLDRLQALE
                     ALADDAAFQDRWRAVKQANKDELAAWMRARYGLPCDPAALLDAQCKRIHEYKRQHLNI
                     LHAIALFERIRTGEDLGVTRTILLAGKAAPAYRAAKLIIRLAHGVAAAIAGDPRARDR
                     LRVVFVPDFSVKTAQRIYPAADLSEQISTAGLEASGTGNMKFMLNGALTIGTLDGANV
                     EIREAVGSEHFFLFGLTADQVAARRPSYRPRDELADSELSRVIDLIASGEFSPGDREL
                     YRPLVDDLLYRDPFFVLADFRSYADCQRQVEDRWRRPASWLRSSILNTARAGRFSSDR
                     AIREYARTIWNVAPVPSRT"
BASE COUNT          454 a         1168 c         1036 g          436 t
ORIGIN      
        1 catggcccgc gcccgcgcgc tgtggcgcgc caagctggcg gccggcggct tcgagcccgg
       61 gccggctccc ggccggagcg ctacgcgacg acgatctcgg tcttgaccga gttggcgatg
      121 aagcagccct cgtgcgcggc gtggtgcagc ctggcgagct cctcggcgct gggctggcgg
      181 tcgccggcgt aggtgatctt cggcgacagc gtcacccggc tgacccacgc gacgccggcc
      241 ggcgtcttgg tcatcacgcc gaccgcgtcg tcgcggtagc tcgcgaccgc gaacccggcc
      301 ttggcggcga cgaacaggaa cgtcagcatg tggcagctcg agatcgcggc gacgtaggcc
      361 tcctcggggt cgaccccgcg cgcgttcgac catggcgcgg gcaccaccga cggcgacgcc
      421 gacgccagga cctcgacgcc gccgtcgaac gtccagcgat gctcgcgcgt gaagcgcagc
      481 ttcaggaagt cgccggcggt ggcgccgccg gcccactcga tcgtggctcg atgttccgac
      541 atgagcgcaa gctacctctt gcgcagcgga tcgccgagcg gtccggagcg cacaccggac
      601 cgcgggcgcc accggcacac ccggtgatgc gctacgttgc cggcatgatc gaccgccacg
      661 atgacacgcg cccgcgcgac gatgccgcgt cgctgcgccg cgatctggtc ggcaagctgt
      721 acttcgagct cgccaagttt cccgaggtcg cgacccgcaa cgatcagttc ctcgcgctcg
      781 cgttcgcggt ccgcgaccgc gtgctgcacc gctgggtcga gtcatcgcgg acctacctcg
      841 agggcaagag ccgcagcgtg gtctacctgt cggccgagtt cctgatcggc ccccagctcg
      901 ccgccaacct gctcgcgctc ggcctgcaca ccgcggcgcg caccgcgctc gccgacctcg
      961 ggatctcgct cgacgagctg gtcgagcacg aggaggagcc cggcctgggc aacggcggcc
     1021 tcggccggct cgccgcgtgc ttcatggagt cgatggcgac gctcgacatc ccggcgatcg
     1081 gccacagcct gcgctacgag ttcggcacgt tcgatcagga gatccgcgac ggctggcaga
     1141 tcgagcacac cgatcgctgg ctgcgcaacg gcaacccgtg ggaggtccgg cggttcgaga
     1201 tcgagcaccc ggtcgggttc ggcgggttcg tcgagcacac caccgacctg cacggccggc
     1261 tgcgcgtgta ctggcacccc gagcgccggg tcaagggcat cccgtacgac gtgccggtcc
     1321 ccggctacgg caccaacacg acctgcttcc tccggctgtg gagcgcggcg gccgacgagg
     1381 agttcaacct cgacgcgttc caggtcggcg agtactggcg cgcggtcgat cagaagatcc
     1441 agagcgagaa cctgaccaag gtgatctacc ccaacgacac cagcccggcc ggcaagcagc
     1501 tccggctcga gcaggagtac ttcttcgtgt cgtgcgcgct gcaggactgc atccggctct
     1561 tgctccagcg cggcacgatc cgcgagttcg ccgacaagtt cgcgatccag ctcaacgaca
     1621 cccacccgtc gctcgcgatc cccgagctga tgcggctgct gatcgacgtc cacgacctgg
     1681 gctggaacga ggcctgggac ctggtgcggc gctcgatcgc gtacaccaac cacacgctgc
     1741 tccccgaggc gctcgagacc tggccgctgc cgctgatcgc gcgcctcttg ccgcgccacc
     1801 tcgagatcat ctacgagatc aaccggcggt tcctcgacga ggtccgcgcc aagttcccgg
     1861 gcgacgaggc ccggctgcgc cggatgtcgc tgatcggcga ggacggcgac aaggtggtcc
     1921 gcatggccca cctcgcgacc gtggcgagcc accacgtcaa cggcgtcgcc gcgctgcact
     1981 cgcgcctgct gcgccagacc gtgctgcgcg acttcgccga gctgtggccg gagcggttca
     2041 ccagcgtgac caacggcgtc acgccgcgcc ggttcgtcgc gctcgccaac ccgcggctcg
     2101 ccgacctgat caccgaggcg atcggcgacg gctggctgcg cgacctcgac cggctgcagg
     2161 cgctcgaggc gctcgccgac gacgccgcgt tccaggaccg ctggcgcgcg gtcaagcagg
     2221 ccaacaagga cgagctcgca gcctggatgc gcgccaggta cggcctgccg tgcgatccgg
     2281 cggcgctgct cgacgcgcag tgcaagcgga tccacgagta caagcgccag cacctcaaca
     2341 tcctgcacgc gatcgcgctg ttcgagcgga tccgcaccgg cgaggacctc ggggtcacgc
     2401 gcacgatcct gctcgccggc aaggccgcgc cggcctaccg cgccgccaag ctgatcatcc
     2461 ggctcgccca cggcgtcgcc gccgcgatcg ccggcgaccc ccgcgcgcgc gatcgcctgc
     2521 gcgtcgtgtt cgtccccgac ttcagcgtca agaccgcgca gcggatctac ccggccgccg
     2581 acctgtcgga gcagatctcg accgccggcc tcgaggcctc gggcaccggc aacatgaagt
     2641 tcatgctcaa cggcgcgctg acgatcggca cgctcgatgg cgccaacgtc gagatccgcg
     2701 aggcggtggg ctccgagcac ttcttcctgt tcggcctgac cgccgatcag gtcgccgccc
     2761 gccgcccgag ctaccgcccg cgcgacgagc tcgccgacag cgagctgtcc cgcgtgatcg
     2821 acctgatcgc cagcggcgag ttctcgcccg gcgaccgcga gctgtaccgg ccgctggtcg
     2881 acgacctcct gtaccgcgat ccgttcttcg tgctcgccga cttccggtcc tacgccgact
     2941 gccagcgcca ggtcgaggac cgctggcggc ggccggcgag ctggctgcgc tcgtcgatcc
     3001 tcaacaccgc gcgcgccggg cggttctcgt ccgaccgcgc gatccgcgag tacgcccgca
     3061 cgatctggaa cgtcgcgccg gtgccgtcac ggac
//
LOCUS       DASVOR010001124         5196 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_332767,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001124 DASVOR010000000
VERSION     DASVOR010001124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332767"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            450..3557
                     /locus_tag="VF516_21475"
     CDS             450..3557
                     /locus_tag="VF516_21475"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8110323.1"
                     /translation="MAAAFALGACAEDNTALPPIHNSPPTLLPLQVSTDEDVPVELAV
                     LGGASDPDGDPLTVASADAEGHLAEIVGPANVKVTPKPNFYGVIHVNYTVSDGHGNLA
                     VGSATVTVRSLNDAPVALGGTQDVRGKSTIFLQASDVDGDTLTYEITAGPTHGTLSGN
                     APQLQYVPDLGFTGDDAITFRVSDGKLASAPATIQLHVAPGGPPTAFDAQSFAFEDEQ
                     AELFLGGSDPDGHPLTFTVVMPPAHGTLSGTAPDLFYTPAPDFNGSDSLQFSVSDGFQ
                     SSAVARVTITVLPLNDPPVAIPQTVAATEDVASNIVLKGTDVDGDALTFELLTFPAHG
                     SLSFTAGSGDHTGATWTYTPAPNYHGPDSFRFNAVDPLSQRSSATVTIDVAPVNDPPV
                     AAPFSVSLAEDASAAVTLRGSDVDGDPLTYTIVTPPSHGTLSGTAPALRYTPALNYNG
                     PDSFTYTTSDSQLTSPSATVTLQVTPVNDPPVAVDGSVTTVEDTPVAITLQASDIDSS
                     TLTFTIATPPSDGTLTGSGTSWTYTPARNATGTRSFTFRVSDGSLTATGKVTIQITPV
                     NDPPTPVDDFVATDAGTPLLIDVLGNDMAADVDGDTLQIDSVDAPAHGTIEIVAGKLR
                     YTPDAGFTGIEVFGYTVIDPQGVTGRAHAHVGVATFPPDAPTEAIATAGGAVTVDDAS
                     RAPAISADGRYIAFTSALALVSDDTNGVEDVYLYDRGTRTITRVSVATDGTQGNAASF
                     RPHLSADGRYTVFDSVASNLVTGDTNGAADVFRHDRVTGETIRVSVAGDGGQASGASF
                     DAVVSEDGNLVVFGSVAFDLVPFGIDTNGAQDVFIRDVTAGTTERISVSNTGGEADLL
                     SGEPAISGDGRFVAFTSQATNLVAGDTNNLFDVFLRDRVAGTTTRVSVSSTGGEASGR
                     CFGPSLSRDGRFISFLSDATDLVAGAPAPVQLYVRDAQGGITTRPVSSSTAIVRARLS
                     GAGRYATAFSSAGTLLRDRFAAVTATLPSASSHWFWPTISGNGRYVVVLDSSNGSVTV
                     TPNPL"
     gene            3604..4692
                     /locus_tag="VF516_21480"
     CDS             3604..4692
                     /locus_tag="VF516_21480"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX8110324.1"
                     /translation="MAPAARCACFGVCLVVFLSAGSLAGIQPALAGKHHVLKASPDDV
                     AWGWLDPKEKPRLTIDSGDTVSIETLMHARDQIHKDVAMDQIVALRKANPGGGPHSLT
                     GPIFVNGAQPGDVLEIRILKIVPKKVGTNFNLPGKDFPTIGALPGDFPDGFVRYFDID
                     NKTHTTVFKPGITIPVRPFPGTLAVGIDPDDPSPRKGGVKDDKLAPVSTLRPWKNGSN
                     MDINELTAGSTIFFPVFQPGALIWTGDSHCAQGNGEVNLTALECSFEEIELQPIVHKD
                     MKLAWPRIETPTHYILVGFDEDLNKAFNNALRETIDFLVADKGLTRYEAYSLASIAAD
                     CRVSQVVDVRKGIHCMIPKTIFTGRPGK"
     gene            4731..>5196
                     /locus_tag="VF516_21485"
     CDS             4731..>5196
                     /locus_tag="VF516_21485"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8110325.1"
                     /translation="MNGARALLQTLVDAGVDVCFANPGTSEMHFVAALDDAPEMRGVL
                     ALFEGVATGAADGYARMTGRPAAVLLHLGPGLANGLANLHNARRARSPVIAIVGDHAT
                     YHKRFDAPLESDIETMARGVSGWFRASRKSRDVAGDAAAAVAAALGPPGQVAT"
BASE COUNT          815 a         1957 c         1663 g          761 t
ORIGIN      
        1 cgcaggggcc ccccgacgtc gccgaccccg ccgccgccct agccgacatc gcgcctcggc
       61 catcccgccg cgccctctgc cgcgccgtgc tcgctgcctg ggccgtcaca gtcaccggca
      121 tgttgtggca tttgacgcac cgcgggcgca ccgtggcgcc gtgaagcgcg ccgcccgtcg
      181 ccgctgcgat cctgctgcgc tccgcgccga tccgcgcggg cagacctccg gcttgactcc
      241 ggcgcgcggc gccactacgg tcgaccccag cgcatgggtc ggcaaggtgg ggaaggcaca
      301 cgaggcatcg ggcctgggcc ccgccaccgc ggaatcgcgt gcccgaacgc ggcgcgtccg
      361 gtccgtcctg catcgaatcc acatcgcgcg ggagctgtat gtcgagtcct gtgaatcggt
      421 cgcgtctcct tccgctggtc gttctggcgt tggctgcggc cttcgcgctg ggggcttgcg
      481 ccgaggacaa cacggcgctc ccgccgatcc acaactcgcc gcccaccctg ctcccgctgc
      541 aggtatccac ggacgaggac gttccggtcg agctcgccgt gctcggtggc gccagcgatc
      601 ccgatgggga tcccctgacc gtggcctccg ccgacgcgga ggggcacctc gccgagatcg
      661 tcggccctgc gaacgtcaag gtgactccga agccgaactt ctacggcgtc atccacgtga
      721 actacaccgt cagcgacgga cacgggaacc tcgcggtcgg gtccgcgacg gtgacggtgc
      781 gctcgctcaa cgatgcgccg gtcgcgctcg gcggcacgca agatgtccgt ggcaagtcga
      841 cgatcttcct ccaggcgagc gacgtggacg gggacacgct gacctacgag atcacggccg
      901 ggccgacgca cggcacgctc tcgggcaacg cgccccagct tcagtacgta ccagacctcg
      961 gcttcaccgg ggacgacgcg atcacgttcc gggtgtccga tggcaagctc gcgtcggctc
     1021 cggcgacgat ccagctccat gttgcgccgg gcggcccgcc gaccgcgttc gacgctcaaa
     1081 gcttcgcgtt cgaggacgag caggccgagc tgttcctcgg tggcagcgac ccggacgggc
     1141 atccgctcac gttcacggtc gtcatgccac ccgcgcacgg aacgctgagc gggaccgcgc
     1201 cggacctgtt ctacacgccg gcgcccgact tcaacggtag cgattcgctc cagttctcgg
     1261 tcagcgacgg tttccagtcg tccgccgtcg ccagggtcac catcaccgta ctgcccctca
     1321 acgatccgcc ggtcgccatc ccgcagaccg tcgcggccac ggaggatgtg gccagcaaca
     1381 tcgtcctgaa aggcactgac gtcgacggcg atgcgctgac cttcgagttg ctgacattcc
     1441 ccgcgcacgg ctcgctgtcc ttcaccgccg gcagtggcga ccacaccggc gcgacctgga
     1501 cctacacacc ggcgccgaac taccacggcc ccgacagctt caggttcaat gccgtggacc
     1561 ccttgtccca gaggtcgtcc gccaccgtga ccatcgacgt ggccccggtc aacgacccgc
     1621 cggtggcagc gccgttctcg gtctcgctgg ccgaggatgc gagcgctgcg gtcacgctcc
     1681 gaggcagcga cgtcgacggt gacccgctga cctacaccat cgtgacgccg ccgagccacg
     1741 gaacgctcag cggcaccgcg ccggcgctgc gctacacacc cgccctcaac tacaacggtc
     1801 cggactcgtt cacgtacacg acctcggact cccagctgac ctctccatcc gccacggtca
     1861 cgctccaggt cacgccggtg aacgacccgc cggtggcggt cgatggctcg gtcacgaccg
     1921 tcgaggacac cccggttgcg atcaccttgc aggccagcga catcgacagc tcgaccctga
     1981 ccttcacgat cgccacgcct ccgagcgacg gtaccttgac cggcagcggc acgagctgga
     2041 cctacacacc cgcgcgcaac gcgaccggca cgcggagctt cacgttccgc gtatcggacg
     2101 gctcgctcac ggcgaccggc aaggtcacca tccagatcac gccggtcaat gatccaccca
     2161 ccccggtcga cgacttcgtg gcgaccgacg ccggcacgcc gctcctgatc gacgtcctgg
     2221 gcaacgacat ggccgccgat gtggacggcg acacgctgca gatcgactcg gtggatgcgc
     2281 ccgcgcacgg gacgatcgag atcgtcgcgg gcaagctgcg ctacacgccc gacgctggat
     2341 tcaccggcat cgaagtcttc ggctacaccg tgatcgaccc tcagggcgtc acggggaggg
     2401 cgcacgctca cgtgggcgtc gcgacgttcc cgccggatgc gccgaccgag gcgatcgcca
     2461 ccgcgggcgg tgctgtcacg gtcgacgacg cctcgcgcgc cccggcgatc tcggccgatg
     2521 gacgctacat cgcgttcacc tcggcgctgg cgctggtgag cgacgacacc aacggcgtcg
     2581 aggacgtcta tctctacgac cgcggcaccc gcacgatcac gcgggtcagc gtggccaccg
     2641 acggcacgca aggcaatgcg gcgagctttc ggcctcacct gtcggcagac ggccgataca
     2701 ccgtgttcga ctccgtcgcg agcaacctgg tcaccggcga caccaacggg gccgcggatg
     2761 tattccgtca cgaccgggtc accggcgaga cgatccgggt cagcgtggcc ggcgacggcg
     2821 gccaggccag cggggcgagc ttcgacgccg tagtctccga ggacggcaac ctggtcgtct
     2881 tcggctccgt ggcgttcgat ctcgtcccgt tcggcatcga caccaacggc gcccaggacg
     2941 tgttcatccg cgacgtgacg gccggaacca ccgagcgcat cagcgtcagc aacaccggcg
     3001 gcgaggcgga cctgctgtcg ggggagccag cgatcagcgg cgacggtcgg ttcgtggcat
     3061 tcacgtcgca ggcgaccaac ctggtggccg gcgataccaa caacctgttc gatgtcttcc
     3121 tgcgcgaccg cgttgctggc acgacgacgc gcgtcagcgt gagctcgacg ggcggcgagg
     3181 ccagcggccg gtgtttcggc ccgtcgctgt cgagagacgg tcgcttcatc tcgtttctgt
     3241 cggacgcgac cgacctggtc gccggcgcgc cggcgccggt ccagctctac gttcgcgacg
     3301 cccagggcgg gatcacgacg cggccggtgt cgtcctcgac ggccatcgtc cgggcccggc
     3361 tgtccggcgc cggacgctat gcgacggcat tcagctccgc cggtacactg ctccgcgacc
     3421 gcttcgcggc cgtcacggcg acactgccga gcgcatcatc ccactggttc tggccgacga
     3481 tctcgggcaa cggccgatac gtggtcgtgc tcgacagcag caacggcagc gtgacggtca
     3541 caccgaaccc gctatgacag acctctggag tagcgtcagg gcctcgactc acaaggagcg
     3601 tccatggccc ccgctgctcg ctgcgcctgc tttggcgtct gtctcgtcgt cttcctcagc
     3661 gctggctcgc tcgccggcat ccagcccgcg ctcgccggca agcaccacgt gctcaaggcc
     3721 tcgcccgacg acgtcgcgtg gggctggctc gatccgaagg agaagccccg gctgacgatc
     3781 gactccggcg acacggtgtc gatcgagacc ctgatgcacg cgcgcgatca gatccacaag
     3841 gacgtcgcga tggaccagat cgtcgccctg cgcaaggcca acccgggcgg cggcccgcac
     3901 tcgctgaccg ggccgatctt cgtcaacggc gcccagcccg gcgacgtgct cgagatccgg
     3961 atcctcaaga tcgtgcccaa gaaggtcggt accaacttca acctccccgg caaggacttc
     4021 ccgaccatcg gcgcgctgcc cggcgacttc cccgacggct tcgtccgcta cttcgacatc
     4081 gacaacaaga cccacaccac ggtgttcaag cccggcatca cgatcccggt gcggccgttc
     4141 cccggcacgc tggcggtcgg catcgacccc gacgatccct cgccgcgcaa gggcggcgtc
     4201 aaggacgaca agctcgcgcc ggtcagcacg ctccggccgt ggaagaacgg ctcgaacatg
     4261 gacatcaacg agctgaccgc cggctcgacg atcttcttcc cggtgttcca gcccggcgcg
     4321 ctgatctgga ccggtgactc gcactgcgcg cagggcaacg gcgaggtcaa cctgaccgcg
     4381 ctcgagtgct cgttcgagga gatcgagctc cagccgatcg tccacaagga catgaagctc
     4441 gcctggccgc ggatcgagac cccgacccac tacatcctgg tcgggttcga cgaggacctc
     4501 aacaaggcgt tcaacaacgc gctgcgcgag accatcgact tcctggtcgc cgacaagggc
     4561 ctgacccggt acgaggccta ctcgctggcg tcgatcgcgg ccgattgccg ggtgagccag
     4621 gtcgtcgatg tccgcaaggg catccactgc atgatcccca agaccatctt caccggccgg
     4681 cccggcaaat aacgctcggg gcgcggcggt ccgcggtagc atgcgccgcc atgaacggtg
     4741 cgcgcgccct gctccagacc ctggtcgacg ccggcgtcga cgtctgcttc gccaaccccg
     4801 gcacctccga gatgcacttc gtcgccgcgc tcgacgatgc ccccgagatg cgcggcgtgc
     4861 tggcgctgtt cgagggcgtc gcgaccggcg ccgccgacgg ctacgcccgg atgaccggcc
     4921 ggcccgccgc ggtgctgctc cacctcggcc ccggcctggc caacggcctg gccaacctgc
     4981 acaacgcccg ccgcgcgcgg tcgccggtga tcgccatcgt cggcgaccac gccacctacc
     5041 acaagcggtt cgacgcgccg ctcgagtccg acatcgagac catggcacgc ggggtgtccg
     5101 gctggttccg ggcgtcgcgc aagagccgcg atgtcgccgg ggatgccgcc gccgcggtcg
     5161 ccgccgcgct cggtccgccc ggccaggtcg cgaccc
//
LOCUS       DASVOR010001125         2023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_40819,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001125 DASVOR010000000
VERSION     DASVOR010001125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40819"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            279..1619
                     /locus_tag="VF516_21490"
     CDS             279..1619
                     /locus_tag="VF516_21490"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8110326.1"
                     /translation="MKPKPARKRSDLRSGSGPSRRQVLQGGLAAAGLVAAGPLVAACK
                     SKSSGTAGKSLLKIGFVSPRTGPAAGFGEPDGYVLELARKALASGLAIGGTTYEVQLI
                     DKDSQSNPQRSAQVANELITGDGVDLMLTTSTPETVNPVSDACEAAGVPCISTVVPWE
                     AWYFGRGAKPDAPSPFKFSFHFCFGVEQFSNAYSHLWPQVETNKKVGVMWPNDADGNA
                     IRRALGPLLTKAGYTIVDPGAYTDGTNDYSSMIAQFKSEQCEIFNTFPIPPDFATFWQ
                     QAAQQSYKPKIAQIAKTGLFPSQVEALGEIGVGLAGGCYWSPTWPYKSLLTGVSSQEL
                     GDGYQKATGKQWNQQLGPSLALFDAAAAALRATGNPKDKAAVAKTLATLEVDTPVGHL
                     AWGKGPFGNVVATPIVGGQWVAAPAGSKYKLDFVICENSSDPKVPVAAQLHAYA"
     gene            1643..>2023
                     /locus_tag="VF516_21495"
     CDS             1643..>2023
                     /locus_tag="VF516_21495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8110327.1"
                     /translation="MTDAQPLLQGRGIVKRYGELVVLDGVDVRIGAGDAVGIVGPNGA
                     GKTTLLGVLAGSVAPSAGSVLLRGDDVTRLPPHVRCRRGIGRAHQVPRPFGGMTVFEN
                     LLVGATAGAGLRGRPAYARCVEVLE"
BASE COUNT          305 a          696 c          729 g          293 t
ORIGIN      
        1 tgcgtctttg ttaccggcgt gcgcccggct ccgcgtccgg ttcgggccca ggatgagacc
       61 agcgtcgtgc ggccggctgg gcaggtgtgt tccgtgaacc gtgattgacg cgccgacgcg
      121 ggcgagccta ccgtgcagca caacgctcgt ccgcgatcca cgtgcctgtt cacgatacgg
      181 gcatccatct tccggcattc cggcggcatt ccggcggcat tccgggggca ttccggaaac
      241 gctgccggga atggctgccg tgtcacggag gtcctgccat gaagcccaag cctgcacgca
      301 agcgatccga tctgcgatcc ggttccgggc cgtcgcgacg acaggtcctc cagggcgggc
      361 tggcggccgc ggggctggtc gccgcgggcc cgctggtcgc cgcctgcaaa tccaagtcat
      421 cggggacggc gggaaagagc ctgttgaaga tcggcttcgt cagcccgcgc accgggccgg
      481 cggccgggtt cggcgagccg gatggctacg tcctggagct ggccaggaag gcgctggcga
      541 gcgggctcgc gatcggcgga acgacctacg aggtccagct gatcgacaag gacagccagt
      601 ccaacccgca gcgcagcgcg caggtcgcca acgagctgat caccggcgac ggcgtcgacc
      661 tgatgctgac gacgtccacc cccgagaccg tcaacccggt atccgacgcg tgcgaggcgg
      721 cgggcgtgcc gtgcatctcg acggtcgtgc cgtgggaggc gtggtacttc ggccgcggcg
      781 ccaagcccga cgcgccgtcg ccgttcaagt tcagcttcca cttctgcttc ggcgtcgagc
      841 agttcagcaa tgcgtacagc cacctgtggc cgcaggtcga gaccaacaag aaggtcggcg
      901 tgatgtggcc caacgacgcc gacggcaacg cgatccgccg cgcgctgggg ccgctgctca
      961 ccaaggcggg ctacacgatc gtcgacccgg gcgcctacac cgacggcacc aacgattact
     1021 cgtcgatgat cgcgcagttc aagtccgagc aatgcgagat cttcaatacg ttcccgatcc
     1081 cgccggactt cgcgacgttc tggcagcagg cggcgcagca gagctacaag ccgaagatcg
     1141 cgcagatcgc caagaccggg ctgttcccgt cgcaggtcga ggcgctcggc gagatcggcg
     1201 tcgggctggc gggcggctgc tactggtcgc cgacctggcc gtacaagtcg ctgctgaccg
     1261 gcgtgtcgtc gcaggagctc ggtgacgggt accagaaggc gaccggcaag cagtggaacc
     1321 agcagctcgg ccccagcctc gcgctgttcg acgccgcggc ggcggcgctg cgcgcgaccg
     1381 gcaatcccaa ggacaaggcg gccgtggcca agacgctcgc gacgctcgag gtcgacacgc
     1441 cggtcggtca cctggcgtgg ggcaagggcc cgttcggcaa cgtggtggcg acgccgatcg
     1501 tcggcgggca gtgggtcgcc gccccggccg gcagcaagta caagctcgac ttcgtgatct
     1561 gcgagaactc gtccgatccg aaggtgccgg tcgcggccca gctgcacgcc tacgcctgag
     1621 ccgtgacaag ctccgctgcc cgatgaccga cgcccagcca ctcctccagg gccgggggat
     1681 cgtgaagcgc tacggcgagc tggtcgtgct cgacggcgtc gacgtccgga tcggcgcggg
     1741 cgacgcggtt ggcatcgtcg gccccaacgg cgccggcaag acgaccctgc tcggcgtgct
     1801 cgccggctcg gtggcgccga gcgccgggtc ggtgctgctg cgcggcgacg acgtcacccg
     1861 gctgccgcct catgtccgct gccggcgcgg catcggtcgg gcccaccagg tgccgcgccc
     1921 gttcggcggc atgaccgtgt tcgagaacct gttggtcggc gcgaccgccg gggcggggct
     1981 gcgcggccgg ccggcgtacg cgcgctgcgt cgaggtgctg gag
//
LOCUS       DASVOR010001126         1772 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_479108,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001126 DASVOR010000000
VERSION     DASVOR010001126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479108"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VF516_21500"
     CDS             complement(<1..1247)
                     /locus_tag="VF516_21500"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8110328.1"
                     /translation="MSPRATQAMMPRYHILLVGIDDYRDRPLGGCVNDIDAVQRLLLD
                     RMGVPSDRIRRLASRHPGSARDPGAPVAERPATLDNLRHELAELGTARVARGDRVFLY
                     FAGHGARIAVRVGSGRTYHREALVLADHDPARADSGLLYDFELNQALRAIAQQTHSVT
                     LVLDCCHAAGVTRGPGELRSPEVLAESEPIARYLDLERMAAVPDPGPPPGDLDVPRAL
                     IAAAEHCHVVAACLGHEYATEECGSDGVRHGLLTSAWLAALDQVPGERLPAVRWSEIW
                     HDVHAAVSQCNPAQHPRMVGNAARAVFAGPPVQHDPGIPVSPATSSASSFRIAAGTLA
                     NITKGAVLAVYGDQPLQFPPVGSDDDRRARIGRVRVTSAELATATAVPEGRAFELPPG
                     ARSRLVDAGVAARLRCAVIPRNAR"
     gene            complement(1244..>1772)
                     /locus_tag="VF516_21505"
     CDS             complement(1244..>1772)
                     /locus_tag="VF516_21505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110329.1"
                     /translation="ATDLPGELELRVLRCPDDRAIPRAEAQDPALSEAAMQDGRYRVA
                     AGSAICVAVRNATPEELQLTLLNVAGDGRVQLLGEASVAPRIRHVFWSRERLGIAFKM
                     ELVAGEERSRDRFVAIGRTTKTHDLKYLAVAETFPQIAVQKGALTKPVGNDGDPSFSL
                     ERWTAAQAVVETFQP"
BASE COUNT          228 a          663 c          652 g          229 t
ORIGIN      
        1 cgggcgttgc gcgggatcac cgcgcagcgc agccgggccg ccacgccggc gtcgaccagc
       61 cggctgcggg cccccggcgg cagctcgaac gcgcggccct cggggaccgc ggtggcggtc
      121 gcaagctcag cggaggtcac ccgcacccgc ccgatccggg cgcgccggtc atcgtccgat
      181 cccaccggcg ggaactggag cggctggtcg ccatagacgg cgagcacggc gcccttcgtg
      241 atgttcgcga gcgtgccggc cgcgatccgg aacgagctcg cggaggacgt cgccgggctc
      301 accgggatcc cgggatcatg ctggaccggc ggccccgcga acacggcgcg cgcggcgttg
      361 ccgaccatcc gcgggtgctg cgccgggttg cattgcgaca ccgccgcatg gacgtcgtgc
      421 cagatctcgc tccaccgcac cgcgggcagc cgttcgcccg ggacctgatc gagcgccgcg
      481 agccacgcgc tcgtcaacag gccatggcgc acgccgtcgg atccgcactc ctcggtcgcg
      541 tactcgtggc cgaggcaggc ggcgacgacg tggcagtgct cggctgctgc gatcagcgcg
      601 cgagggacgt ccaggtcgcc gggcggcggc cccggatccg gcaccgcggc catgcgctcg
      661 aggtcgaggt agcgcgcgat cggctcgctc tcggcgagca cctcggggga gcgcagctcg
      721 ccggggccgc gggtgacgcc ggccgcatgg cagcagtcga gcaccagcgt gaccgagtgg
      781 gtctgctggg cgatcgcgcg cagcgcctgg ttgagctcga agtcgtacag caatccgctg
      841 tccgcccgcg ccggatcgtg atcggccagc accagcgcct cgcggtgata ggtccgtccg
      901 ctgccgaccc ggacggcgat gcgcgccccg tgacccgcga agtagaggaa cacccggtcg
      961 ccccgcgcga cccgcgccgt gccgagctcg gcgagctcgt ggcgcaggtt gtcgagcgtc
     1021 gcgggtcgct cggcgaccgg cgcgccggga tcccgggcgc tcccggggtg ccgcgacgcc
     1081 aggcgccgga tccggtcgct cggcacgccc atccgatcga gcagcagccg ctgcacggcg
     1141 tcgatgtcgt tgacgcagcc gccgagcggc cggtcgcggt agtcatcgat gccgaccagc
     1201 aggatgtgat agcggggcat catggcctgg gtcgcgcgcg ggctcatggc tgaaacgtct
     1261 cgaccaccgc ctgcgccgcg gtccagcgct ccagcgagaa gctcggatcc ccgtcattgc
     1321 ccaccggctt ggtcagcgcg cccttctgca cggcgatctg cgggaacgtc tcggcgacgg
     1381 cgaggtactt gaggtcgtgg gtcttcgtcg tgcggccgat cgccacgaag cgatcgcgcg
     1441 atcgctcctc gccggcgacc agctccatct tgaaggcgat cccgagccgc tcgcgggacc
     1501 agaacacgtg gcggatcctg ggcgccacgc tggcctcccc gagcagctgg acccggccgt
     1561 ccccggcgac gttgagcagc gtcagctgga gctcctccgg cgtcgcgttg cgcacggcca
     1621 cgcagatcgc gctgccggcc gccacccggt atcgcccgtc ctgcatcgcc gcctcggaca
     1681 gcgccgggtc ctgcgcctcc gcgcgcggga tcgcgcgatc gtcggggcag cgcagcacac
     1741 ggagctccag ctcgcccggc aggtcggtcg cg
//
LOCUS       DASVOR010001127         4398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362215,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001127 DASVOR010000000
VERSION     DASVOR010001127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4398
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362215"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VF516_21510"
     CDS             <1..674
                     /locus_tag="VF516_21510"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8110330.1"
                     /translation="RPAHAVQRRYRGNTLILETEFENDEGVVRLIDFMPIRRSRHQAP
                     RVIRILEAVRGQVTVRFDLRPRFAYGYTIPIELSRDSTSSAVAGPDALYLRGGRTGAP
                     PFQTDLVLDEGERITYELAWARPYDDPPQPVDVGAVLRETEEFWSDWSAKIRLPSEYK
                     DIVMRSLITLKACTYAPSGAIVAAPTFSLPETPGGERNWDYRFTWVRDSMLTLNAACL
                     REVVA"
     gene            643..1257
                     /locus_tag="VF516_21515"
     CDS             643..1257
                     /locus_tag="VF516_21515"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8110331.1"
                     /translation="MPHVSGKLSPEPAWVAIDRWVRLIDLLDLDEDRARWESLREAIR
                     NEILDKGYDANRNTFTQYYGSKNVDASLLFIPLTGFLPATDPRVVGTVKAIEEDLMQD
                     GLVLRYRTDASDDGLSGEEGVFLACSFWLAVTYHLMGRTEDARRLFERVANLRNDVGL
                     LAEEYLTSQKRQIGNFPQAFSHLALVNAAYVITENHSPAEHPEA"
     gene            1403..2737
                     /locus_tag="VF516_21520"
     CDS             1403..2737
                     /locus_tag="VF516_21520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8110332.1"
                     /translation="MRPGTLDQIVGGATGRRAQSDPSLTMQAPHGMQTVQDYEPFIGR
                     EAVDRILEKGRRLSDRTVAHVNSTYYGGGVVGLLSPLTLLMRSLGLEAEWRAIQGPPD
                     FFTTTKKMHNALQGAPIHLTELKKDIYEGVALQNAVRNRFDHDFVVVHDPQPLPLVRF
                     SRKRGPWIWRCHIDLAHPSREIWEYLSSFIEQYDAVIESADEYRQPWAVPQLVFQPAI
                     DPFSIVNCELSDEQIDQRLEHYGIPTDLPLVVQISRFDRWKDPQGVVQAVQLARRVRD
                     CTLVLLGNLANDDPEGPMVYESLLGLRGERVLVLSCEDTALVNALQRRAAVVVQKSLR
                     EGFGLTVTEAMWKGTPVVGGNVGGIPCQIEDGINGFLVSSVEQTADRIVRLLSDESLR
                     RQMGEAARETVRERFLMTRYLEQYLDLFESFETIYRLRPRQVAVMTEAVVEH"
     gene            complement(3160..3849)
                     /locus_tag="VF516_21525"
     CDS             complement(3160..3849)
                     /locus_tag="VF516_21525"
                     /inference="COORDINATES: protein motif:HMM:NF013003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8110333.1"
                     /translation="MTPGWAAFVVAGALALSVGLGIIVGLSARYVRGLYTSGDVRLEM
                     IALFVGASLLVFLAAGVWRGAGFATRNVLPVTIALAIASALIGIVTGAGSGVGGLVAL
                     VFVVVAAIIVTLAVLARAVAGTAGKVMFSLVAIAGGLAGGASGGGLAAAVVAICAMLM
                     ARRGHTPLLERTTAAVASSHGTSFRNANLAGANLHGARLVACDFRGANLQGTHLDRQA
                     TKLCRFDEDAS"
     gene            4181..>4398
                     /locus_tag="VF516_21530"
     CDS             4181..>4398
                     /locus_tag="VF516_21530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110334.1"
                     /translation="MQLTANQRSRRQPGQVGDRDEPWLGLSPKVTEDMFGRGQDIPQA
                     RRHYGMASLTVRRSDSRLRFVQCVYPAVV"
BASE COUNT          777 a         1462 c         1462 g          697 t
ORIGIN      
        1 taaggcccgc gcacgcggtc cagcggcggt atcgaggcaa cacgctgatc ctcgagaccg
       61 agttcgagaa cgacgagggt gtggtccggc tgatcgactt catgccgatc cggcgcagcc
      121 ggcatcaagc gccccgcgtg atccggatcc tcgaggctgt gcgcggacaa gtcacggtgc
      181 gattcgacct gcgtccgagg tttgcgtacg gctacacgat ccccatcgag ctgtcgcgag
      241 acagtacctc ctcggccgtc gccggaccgg atgcgctgta tctccgcggc ggacggacgg
      301 gggcacctcc gttccagacc gacctggtgc tcgacgaggg ggaacgcatc acctacgagc
      361 tcgcttgggc acgcccttac gacgatcccc cgcagcccgt cgatgtgggc gcagtgttgc
      421 gcgagaccga ggagttctgg agcgactgga gcgccaagat ccggctgccg tccgagtaca
      481 aggacatcgt catgcgctcg ctgatcacgc tcaaggcctg cacctacgct ccaagcggag
      541 cgattgtcgc ggcgcccact ttcagcctcc cagaaacacc aggtggcgaa cggaactggg
      601 actaccggtt cacgtgggtc cgtgactcga tgctgacgct gaatgccgca tgtctcaggg
      661 aagttgtcgc ctgagccggc atgggtcgcg atcgaccgat gggtccggtt gatcgaccta
      721 ctcgacctgg acgaagacag ggcgcgatgg gaaagccttc gcgaggcgat ccgcaacgag
      781 atcctcgata agggttacga tgcgaaccgc aatacgttca cgcagtacta cggctcgaag
      841 aatgtcgatg cgagcctgct gtttatcccg ctgacgggat tcttgccggc cacggatccg
      901 cgcgtcgtcg gcaccgtgaa agcgatcgag gaagacctga tgcaggacgg gctggtgctc
      961 aggtaccgta ccgacgccag tgacgacgga ctctccggtg aggaaggcgt gttcctcgcg
     1021 tgctcgttct ggctcgcggt cacctatcac ctcatggggc gcacggaaga cgcgcgccgg
     1081 ctgttcgagc gagtcgcgaa cctgcgcaac gatgtcggtc tgctcgccga ggagtacctc
     1141 accagtcaga agcgccagat cggcaacttc ccgcaggctt tcagccatct cgccctcgtc
     1201 aacgcggcat acgtgattac ggagaatcat agccccgccg agcaccccga ggcttgagcc
     1261 gctacggccg gacaagcgcg tacgacgcgc atcgcgctcg cggcactgaa ccgcccgggg
     1321 ttcgtcggag gctcccgaag gacgcgcgct gcctcggcat cggcctgtga ggaagcgctc
     1381 gatcgtgcca gtacgtatcc ggatgcggcc cggcacgctc gaccagattg tggggggagc
     1441 gaccgggcga cgcgcgcaat ccgatccatc cttgactatg caagctccac acggaatgca
     1501 gactgtccag gactacgagc ccttcatcgg ccgcgaggcc gtggaccgga tcctcgagaa
     1561 agggcggcgc ctctccgacc gcaccgtcgc acacgtcaac tcgacgtact acgggggcgg
     1621 cgtcgtcggg ttgctgtcgc ctctcaccct gctcatgcgc tcgctggggc tcgaggccga
     1681 gtggcgggcc atccaagggc cgccggattt cttcaccacc accaagaaga tgcacaacgc
     1741 gctacagggc gcgccgatcc acctcaccga gctaaagaag gacatctacg agggggtcgc
     1801 gctccagaac gcggtccgta atcgcttcga tcacgacttc gtcgtcgtcc acgatcccca
     1861 gccattgccc ctcgtgcggt tctcgcgcaa gcgcgggccg tggatctggc gttgccatat
     1921 cgatctcgcg caccccagcc gggagatctg ggagtacctc agcagcttca tcgagcagta
     1981 cgacgcggtg atcgagagcg ccgacgagta ccgacaaccg tgggccgtgc ctcagctcgt
     2041 gttccagccg gcgatcgatc cgttcagcat cgtcaactgc gagctttccg acgagcagat
     2101 cgatcagcgc ctcgagcatt acggcatccc caccgacttg ccgctggtcg tacagatctc
     2161 tcggttcgat cggtggaagg atccccaagg cgtggtgcaa gcggtccagc tggcgcggcg
     2221 tgtgcgcgac tgcacgctgg tgctgctcgg caaccttgcg aacgatgacc cggaggggcc
     2281 gatggtctac gagtcgctgc tcgggcttcg cggggagcgg gtgctcgtgc tcagctgcga
     2341 ggacacggcg ctggtcaacg cgctccagcg ccgtgccgcg gtcgtggtcc agaagtcgct
     2401 gcgcgagggc ttcgggctca cggtcacgga ggcgatgtgg aaaggcacac cggtcgtcgg
     2461 cggtaacgtt gggggcattc cttgccagat cgaggacggc atcaatggct tcttggtgtc
     2521 ctccgtggag cagacggccg atcggatcgt gcgcttgctc tcggacgagt cgctgcgccg
     2581 ccagatgggt gaagcggcgc gcgaaaccgt gcgagaacgg ttcctgatga ctcgctacct
     2641 cgagcagtac ctggacctgt tcgagtcgtt cgagacgatc tatcgcttgc gaccgcggca
     2701 ggtggcggtc atgaccgagg cggtcgtcga acattgatgc aaccgatccg ggacgacgag
     2761 aggtccacgc gctgtcgccg cggtcgacca gcagctccga gatccgcgcg tcctcgaacg
     2821 gcaggtggcc gttcgatccg ggaaacccgg tcgcgtgctc cggcaggcag gccacatgcc
     2881 gttcgcgccc gcgtgcggcc gttcagcagg agcgcgtggc cgagccgagg ccggtcgtgt
     2941 ggaactgcgt gtagcgcagc ccgcgactcg ccagccgcga tatcgtcgtc ggcgtgtcga
     3001 tcaggccacc gtagcagtcg aacgcgccga acccgacatc gtcctagacg atgaagagga
     3061 gtttgggagc tcgggccggc gcgcggtgca gtccgtaggg gtgcccagtc ggggtcgact
     3121 cgcgcgcgtc gaccgagagc cggccgcgaa acacggtctt cacgacgcat cctcgtcgaa
     3181 gcgacacaac ttggtcgcct ggcggtcgag gtgtgtcccc tgcaggttgg cgccgcggaa
     3241 gtcgcacgcg acgagccggg cgccgtggag gttggcgccc gcgaggttcg cgttgcggaa
     3301 cgaggtgccg tggctgctgg ccaccgcagc ggtcgtccgc tcgaggagcg gtgtgtggcc
     3361 gcggcgcgcc atcagcatcg cgcagatcgc gacgaccgct gccgccaggc caccgccgct
     3421 ggcgccgccg gcgagcccgc ccgcgatcgc gacgagcgag aacatgacct tccccgcggt
     3481 gcccgcgacc gcgcgcgcga gcacggcgag cgtgacgatt atcgcggcga ccacaacgaa
     3541 cacgagcgcg accagcccgc cgacaccgct gcccgcaccg gtgacgatac cgatcagggc
     3601 cgacgcgatc gcgagcgcta tcgtcaccgg cagcacgttt cgcgtcgcga agccggcgcc
     3661 cctccacaca cccgccgcga ggaacaccag cagggacgcg ccgacgaaca gcgcgatcat
     3721 ctccaaccgc acgtcgccgc tcgtgtacag cccgcgcacg tatcgcgcac tcagcccgac
     3781 gatgatgccg aggccgaccg agagcgcgag cgctccggcg accacgaacg ccgcccaacc
     3841 tggcgtcatc cccgagcgga ttcgcgaaag gtcggcgtcg tggaggtcgg cgcccgagaa
     3901 gtcagcgcca cgcagatcga agccagagag atgtgccgat gacaagtttg cgcccgccat
     3961 cgaacgccgg cgcacgtcag ccgtcgagac gtccatgacc cagtggggta gcaagatcgg
     4021 gaccctcaga tcgatgcttg gcgttgacga cggtggggac gtgcccctac ttctacgcca
     4081 aagccgtacg acgaccgcgc gcgatcttcg aggcagcctt tccacaagcg cccggtgtcc
     4141 tcggggaggc cggcgctcga gctggtgacc ctcttgcaac ttgcaactca ccgcgaacca
     4201 gaggtcaagg cggcagcccg gccaggtcgg cgatcgcgac gagccgtggc tcggcctgtc
     4261 gcccaaggtc accgaggaca tgttcggccg gggccaggac attccgcaag cgcggcgtca
     4321 ctatggcatg gcctcactca cggtccggcg atcagacagt cgtcttcgat ttgtccagtg
     4381 tgtctatccg gcagtagt
//
LOCUS       DASVOR010001128         2692 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_12212,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001128 DASVOR010000000
VERSION     DASVOR010001128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2692
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12212"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VF516_21535"
     CDS             <1..601
                     /locus_tag="VF516_21535"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8110335.1"
                     /translation="PGITLEQFNKLGQPIDSPEAADVLAKYLVGDLLWRSLRAADATS
                     QGISGTAPSIFISYSRQNGDWVSRLRTLLRPVERRGLLSSWVDADIEPGLSWENQLMS
                     KLGSAQAALLLVSPALLGSEYVQKKEIPVLVERMKGDKNFRLFWTLIEACEWQTIPEL
                     QEIQAIGDVKIPISASPTKSDEQCRLIELVEKIATKLAV"
     gene            complement(856..1599)
                     /locus_tag="VF516_21540"
     CDS             complement(856..1599)
                     /locus_tag="VF516_21540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110336.1"
                     /translation="MTAEIPSWLRPHFQPGGGDPFLFYKIHGAFAEPLELSLSKHRSA
                     GVPVGCELQLLTTREALDFGLDRGWIGDAFRAEQPVLAAEVARTEQCLVLRGSPGDPD
                     TLDYFRDAVGLVMAVLDSGGIAVFDPQMFKWWSTAEWRRDAFEPAGAVPHHHVVILAS
                     EDSHGCWYHTRGLRKFGRPDLSIHDVPPELEPAVRELCDRFIEMLAFGAVIPDGQLIR
                     MPGLPAWKCRHAGDFSDPDFNNVHIEIGP"
     gene            1994..>2692
                     /locus_tag="VF516_21545"
     CDS             1994..>2692
                     /locus_tag="VF516_21545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110337.1"
                     /translation="MDQPSPPAADWVIAVQPDSPPAGRLSPALLGHYDLSGSLLGYAA
                     NAPLIDRMQAIGFSEWRVGLGRWEFATRLLPTLADGHNTSCAGQLAALPPTVRAPAGA
                     TDDTLIADRDWFIDNGQPVSAADTLDDRRYRLDYIRGVLDTAAAFGAAPFVDIDHMPR
                     ALGVSRTFFRGGVVQGVTDPCLTTWTNHVSNTRPADPGVFASAVVGAVRRVVEGSSGA
                     APRTARYWELWNEPE"
BASE COUNT          442 a          897 c          922 g          431 t
ORIGIN      
        1 ccccggcatc acgctcgaac agttcaataa attaggccag ccgatcgatt caccggaggc
       61 cgccgacgtc ctcgcaaagt atctagttgg cgatctgctg tggcggtctt tacgtgcggc
      121 cgacgcgacg agccaaggca tctcggggac tgcaccttcg atctttatca gctacagccg
      181 acaaaatggg gactgggtgt ccaggctgcg gaccctgctg cggcccgtcg aacggcgggg
      241 gcttctgtcc agctgggtcg atgccgacat cgagccgggt ctgtcatggg agaaccaact
      301 catgtcgaag ctcggcagtg cgcaggccgc attgctgttg gtcagccccg ccctgttggg
      361 gtcggagtac gtccagaaaa aggagattcc ggtgcttgtc gagcgaatga agggcgacaa
      421 gaattttcgc ttgttctgga cactaatcga agcatgtgag tggcagacca ttcccgagtt
      481 gcaagagatt caagccatcg gcgatgtaaa aatacccatc agcgcaagcc cgacaaaaag
      541 cgatgaacag tgccggctga tcgagcttgt cgagaaaata gcgacaaaac tcgccgtata
      601 gagtgaccca gtgcgccact cgcacgctgc tacccgccca cgtcgagact cgccgcgcga
      661 gcgccgcacc ggtcggagtt accagaactg cccaaactgc gacccgaccc cggccggccg
      721 attcgagtcg cgttgcagtt ctgcgcgtta tggtaactgc gagcgggcgg gctgttctaa
      781 tattttgact tccccgcaga atccgacatc gatcctcggc ggagcccccc gtgatgagtt
      841 ctgcatggct gccgatcagg ggccgatctc gatgtggacg ttgttgaagt ccggatcgga
      901 gaagtcgcca gcgtgccggc acttccaggc gggtaagccg ggcatccgga tcagctgccc
      961 gtcggggatc accgctccga acgcgagcat ctcgatgaag cggtcgcaca gctccctgac
     1021 cgccggctcg agctccggcg ggacgtcgtg gatgctgagg tccgggcggc cgaacttgcg
     1081 caaaccgcgc gtgtggtacc agcagccgtg gctgtcctcg ctggcgagga tcacgacgtg
     1141 atggtgcggc accgccccgg cgggctcgaa cgcgtcgcgg cgccactctg cggtgctcca
     1201 ccacttgaac atctgcggat cgaacacggc gatgccgccg ctgtcgagca cggccatcac
     1261 gaggccgacc gcgtcgcgga agtagtcgag ggtgtccgga tcaccggggc tgccacgaag
     1321 cacgaggcat tgctcggtgc gcgcgacctc ggcggcgagc acgggctgct ctgcgcgaaa
     1381 cgcgtcgccg atccagccgc ggtcgagtcc gaagtcgagg gcctcgcgtg tcgtgaggag
     1441 ctgcagttcg cagcccacgg gcacgccggc ggagcgatgc ttcgagagcg agagctcgag
     1501 cggttccgcg aacgcgccgt ggatcttgta gaacaggaat gggtcgccgc ctccgggctg
     1561 gaaatgcggg cgcagccagg aggggatctc ggcggtcatc cggatacact actggaaccg
     1621 cgctgcccgc agcggcgcga gcatgtcgag cccctcgttg atgctgccca cgggttggaa
     1681 ggggcggaca gtgccgcgcc ccacgacctt ggggtgggat tcacatcgct caccgttcgc
     1741 tcggcgcctg gatggggcga aggaccggcg atcggcgcgc atgtggaaac ctccgccatc
     1801 cgacggcctc cggacgcagc gcggcggtcc cgcgagcgta ggatttcacc acggcgacgc
     1861 agatcttcgc gaaacccggg atgccggcgc acggcacggc gcttgctgct cggggcgtgt
     1921 ggccaggtgt cttctcctcc tcgcgtcgct cgccccttgc ggcgtcggcg gaccgcagcc
     1981 ggacgcgccg ccggtggacc agccgagccc gccggccgcg gactgggtca ttgccgtgca
     2041 accggacagc ccgcccgccg gtcggctgtc gccggcgctg ctcggccact acgatctctc
     2101 gggctcgctg ctgggatacg ccgcgaatgc gccgctgatc gatcggatgc aggcgatcgg
     2161 cttctccgag tggcgcgtcg gcctcgggcg ctgggagttc gccacgcggc tgttgccgac
     2221 gctggccgac ggccacaaca cctcgtgcgc cgggcagctc gcggcgctgc cgccgacggt
     2281 tcgcgcaccc gccggcgcga ccgacgacac gctgatcgcc gatcgcgact ggttcatcga
     2341 caacgggcag ccggtctcgg cggccgacac gctcgatgac cgccgctacc ggctcgacta
     2401 catccgcggc gtgctcgaca ccgcggcggc gttcggcgcg gcgccgttcg tcgacatcga
     2461 ccacatgccg cgcgcgctcg gcgtgagccg cacgttcttc cgcggcggcg tggtccaggg
     2521 ggtcaccgat ccgtgcctca ccacctggac caaccacgtc tcgaacacgc gcccggcgga
     2581 tccgggcgtg ttcgccagcg cggtggtcgg cgcggtgcgc cgcgtcgtcg agggctcgag
     2641 cggcgcggcg ccgcgcaccg cgcgctactg ggagctgtgg aacgagccgg ag
//
LOCUS       DASVOR010001129         9823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_216113,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001129 DASVOR010000000
VERSION     DASVOR010001129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9823
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216113"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2577
                     /locus_tag="VF516_21550"
     CDS             <1..2577
                     /locus_tag="VF516_21550"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110338.1"
                     /translation="APELAAEFHAVLRAQPREQLRSAAQHWQDRARPRSLLWSRDVLA
                     EVERWTQPASRKLSELECSFVAASQRRARRIRWVRRSLAMLAVAGVFGALQVRSAMQA
                     DLAEQRTRLAEQRTRSAQQLAETRVTQAELEQGRAALLHGEPEAQAHLTEAYRRDPSS
                     STAFMLARAIQPRLMEQARFASTHGRMWSAAFSPDGQQIVTTDDRAAQIWDANTHQLR
                     FTLPHGCEVYDATYSPDGAWLVTAARTAVKRWDARSGRLVRTWNVAPGSGAPSDYYRA
                     AISPDGRLVAAIDAVGSAAQVWNAASGERVAELHNRAAGFPAIAFSSQGWLATTGGEE
                     VRVFDVRTWQPVATLPGPVHSLAFDARTHLVTGAATGEIAIWALPSGARLQDLRRFGE
                     SVEAVAFSTDGQRVAAGSRDGATQVWHAASGALQSQLNPRHSKVLAVEFDRTARFLLA
                     AGADGSVVVADAAQGLPVAVLDGPGSVVRVAHFDPSSRSIVGASWDGTARVWDASSPY
                     RRWSSAPISNACAGGMGSEPDRRFIAIGCGDLPIQLWDTAQDQPVAELPSVTRIEGGD
                     FTSAAPAVSAAGDRAAIARNRVVEVYQLPARRLLRAVPHSAPVSSVAYASTGRDLISG
                     AVDGLLLITHDDGTQRSLHAPAGIDAAELLPDGRAVVSDALRHLRIYGPGSTVLADLE
                     LPTRVMSLRGDQTRLVAIPSYLSEASPPLLIDLERYRVIAQLAGHVGQVFSARWVAGG
                     RILTAGADGTARMWDGVTGRLLQVYRGGSRFLGDVTLMTDDVVIGGDADGVLRFWDAT
                     SGAKLWALQAHKSAIIGVHVEGNGVVTRGFTGEISRWRLPAAEEVIGACGNHPHCAIV
                     AQ"
     gene            2574..2741
                     /locus_tag="VF516_21555"
     CDS             2574..2741
                     /locus_tag="VF516_21555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110339.1"
                     /translation="MTKSPRKLVLHKETLRALTSMELRRAIGGADGNNQVDNTFNKNC
                     PAVNMIVAPGQ"
     gene            3096..3482
                     /locus_tag="VF516_21560"
     CDS             3096..3482
                     /locus_tag="VF516_21560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110340.1"
                     /translation="MRRIILILLTLVLAPSVATASWYRCAYDGTIRSVCCCPTKPDQH
                     KKNAPPPDTSLRAACCCTVIQATSNAREIEASTPAQSKLAPMALAVPTVVAPRPAPIL
                     AVALDRPRAQGDPPDTLFTRRCLLLL"
     gene            3737..4894
                     /locus_tag="VF516_21565"
     CDS             3737..4894
                     /locus_tag="VF516_21565"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8110341.1"
                     /translation="MDGALDRAAVVAAVLAHNPDLDAVRATWRAAVAAFPSAISLDDP
                     VASYALAPFSIGSDARFGQRIEISQKLPWPGKRELRGDAALADAEAAQADFESLQLDL
                     AEAAVQAFDDDYIATRALDINQHHRELLERIERSALAQYAAGRASQQDSLEARAHIIE
                     LDRERLMLERQRRVAVATLNRLLHRKADAELPPLPARLAVSRSTEPAHEHPKQLAAAA
                     RIRARQAEVDGAALAFYPDFEVMGSYDSMWDTWQHRWMIGVGIDIPLQRGKRRADLER
                     ARAEQARAEADLASVTDMLDERRERVRREVDESTRALELYEQQLLPTTRERVDAALAG
                     FTSGQNPFSTVVMAEHELRDVELAIERARADLDRQLAALDRLEGRIPGSGR"
     gene            5002..6651
                     /locus_tag="VF516_21570"
     CDS             5002..6651
                     /locus_tag="VF516_21570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8110342.1"
                     /translation="MSAAVIGALILLAVVFRHPLIAWFTGSSSGGGSTSAAAHVAAAG
                     LSIDTSIEPDPPRENGDRAHVLVRDAQDKPVSGAKVRLEYDMPAMGTMQAMHGGVDAS
                     EDGPGRYTIPFDLGMGGSWTISIHVKTASATATARYTLRVGSSGLTVLGGESGRAGRG
                     GSGSMGGMNGTDSGSDISYYTCSMHPTVHSKEPGKCPICSMDLTPVMRDEEQAGVIHI
                     DENRRALLGIRTTKVVRRPIDLEITAKGRLAVDETRLHDVVLKVGGYVSDLRVNATGQ
                     SVARGQTLFTLYSPELYAAEQEYLIANQNREAMRVNGDASHSERLAHAAETKLRLWGL
                     DDDQIAELARHGEPIERVPFKSPASGVVVEKNVVDGASVMAGQRLFRVADLTDIWVEA
                     DVYESDLPRITKNLPASITLDYLPGKMFEGKVAFIYPYLDPASRTGRVRVALANQGLE
                     LKPDMYATVTFKLPLGPRLVVPISAVVYTGPRRIVFVDLGNGALRPQEVTIGARSGDL
                     IEITTGLGEGDIVVSSGNFLVAAESRVRSAGSFWEDSHGDQ"
     gene            6638..9766
                     /locus_tag="VF516_21575"
     CDS             6638..9766
                     /locus_tag="VF516_21575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX8110343.1"
                     /translation="MATSNDQPGIIGRVIAGSASNPLITIMLVIGLGVWGWLSLRRAP
                     LDAIPDLSDAQVIIFTEWMGRSPDLVEDQITYPISSSLIAAPGVKYVRGQSMFGMSFV
                     YVIFEDGTDIYWARSRVLEYLNQAQAQLPAGVTPRLGPDATGVGWVFEYALVDRTGKH
                     DAQQLRSLQDWNLRYALASVKGVAEIASVGGAVKQYQVNVDPQKLLALKVPLRDVMIA
                     LQRSNADVGGQVMEIAGHEHVIRGRGYVHARTDLEEVPLRTTDAGVPVRVKDIGEVEI
                     GQDIRRGLTDLDGEGEVPGGIVIMRYGENALDVIDGVKARLAEIQRTLPEGVQVVVTY
                     DRSALIRASIDTLQHTLIEEMIVVALIIFLFLLHVRSAVIPILTLPLGVLLAFIPMAY
                     QHLTANIMSLGGIAVAIGAMVDASIIIIENIHKKLDDWERHGRPGPRLGAITAAMQEV
                     GPSIFFSLLVITVSFIPVFTLQGTEGRLFKPLAFTKTYSMGFAAVLAVTLTPALAAIF
                     VRGKIPREDRHPVNRLLTRAYAPVVRWAVRYRGLVIALAALVIAFTIPAWFRLSSEFM
                     PPLNEGAILYMPTAPPGMSIAEASHLMQVMDKELKSFPEVASVFGKNGRAETPTDPAP
                     FGMVEATVVLKPHDQWRPGMTWDRLVAEMDAKLHYPGMPNIFWMPIQTRTEMLSTGIR
                     SPLGIEVFGDNTDQIEKAAVAIESAVVNVPGTRSAFADRSTGGFYIDLVVKRAAAARL
                     GLTVGDVEEVIQTAIGGMNVSETVDGRERYSINLRYAREFRDDPSMLERVLVPTPSGA
                     QVPLSQVAEVKTVTGPPMIRSEDGKLVGYVFLDTTRPMSDYIADAQRVVAGLQLPKGV
                     RVGWVGQFQYLERAKARLKIVVPITLMIVFFPIFLNTRSLIETGIVLLAVPFSLVGAI
                     WLIYLLHYNISVAVWIGMIALAGLDAETGVVMLLYLTLAHRAWKAEGRLNTPDDLRES
                     IVEGAAHRIRPKLMTVLTMMVGLLPVLWSSGTGADVMKRITAPMVGGLVTSFVLELLV
                     YPAIFAMWKARA"
BASE COUNT         1548 a         3330 c         3351 g         1594 t
ORIGIN      
        1 gcgccggagc tcgccgccga gttccacgcc gtgctgcgcg cgcagccccg cgaacagctg
       61 cggtcggcgg cgcagcactg gcaagatcgc gcacggccgc ggagcctgct gtggagccgc
      121 gacgtgctcg ccgaggtcga gcgctggacg cagccggcgt cgcgcaagct cagcgagctc
      181 gagtgctcgt tcgtggcggc gagccagcgg cgcgcccgcc ggatccggtg ggtccggcgc
      241 tcgctggcga tgctcgcggt ggccggcgtg ttcggcgcgc tccaggttcg ctcggcgatg
      301 caggcggatc tcgcggagca gcggacccgt ctggccgagc agcggacccg ctcggcgcag
      361 cagctcgcag agaccagggt cacccaggcc gagctcgagc agggtcgtgc ggcgctgctc
      421 cacggggagc cggaggccca ggctcacctg accgaggcgt acaggcgcga tccgtcgtcc
      481 agcacggcgt tcatgctggc gcgcgcgatc cagccccgcc tgatggagca ggcgcggttc
      541 gcgagcaccc acggtcgcat gtggtcggcc gcgttctcgc ccgatggcca gcagatcgtc
      601 accaccgacg accgggcggc gcagatctgg gacgcgaaca cccaccagct gcggttcacg
      661 ctgccgcatg gctgcgaggt ctacgacgcc acctacagtc cggatggagc gtggctcgtc
      721 accgcagcgc ggacggcggt caagcgctgg gacgcgcgca gcggacggct cgtccgcaca
      781 tggaacgtcg cgccggggag cggagcgcca tcggactatt accgcgctgc gatctcgccg
      841 gatggacggc tcgtcgcggc gatcgacgcg gtgggatccg cggcgcaggt ctggaacgcc
      901 gccagcggcg agcgggtcgc ggagctgcac aaccgagccg ccggcttccc cgcgatcgcg
      961 ttcagctcgc agggctggct cgcgaccacc ggtggcgagg aagttcgcgt gttcgacgtc
     1021 cggacctggc agccggtcgc caccctcccg ggccccgtcc acagcctggc attcgatgcg
     1081 cgcacccatc tggtcaccgg tgccgctacc ggcgagatcg cgatctgggc gctgccgagc
     1141 ggcgcacgac tccaggacct gcgccgcttc ggcgagtcgg tcgaggccgt cgcattctcg
     1201 acggatggtc agcgggtcgc cgcgggcagt cgcgatggcg cgacgcaggt ctggcatgca
     1261 gcgtcgggag ccctgcagag ccagctcaat cctcgtcaca gcaaggtcct cgcggtggag
     1321 ttcgatcgga ccgctcggtt cctgctcgcc gccggtgccg acggcagcgt cgtggtcgcg
     1381 gacgccgcgc agggactgcc cgtcgcggtc ctcgacggcc ccggcagtgt cgtgcgggtc
     1441 gcgcacttcg acccgagctc ccggagcatc gtgggcgctt cctgggatgg gaccgcccgg
     1501 gtgtgggacg cgagctctcc ctatcgccgc tggagctcgg ccccgatcag caatgcctgc
     1561 gccggcggga tgggctccga gcccgatcgc cgattcatcg ccatcggctg cggcgatctc
     1621 ccaatacagc tgtgggatac ggcccaggat caaccggtcg ccgagttgcc gagcgtgaca
     1681 cgcatcgagg gcggcgactt cacgtccgcg gccccggccg tgtccgcggc gggggatcgc
     1741 gccgcgatcg ctcgcaaccg ggtcgtggag gtgtatcagc tcccggcgcg ccggctgtta
     1801 cgagccgtcc cgcacagtgc tcccgtgagc tcggtcgcat acgcgagcac ggggcgagac
     1861 ctgatcagcg gagcggtgga tggcttgttg ctgatcacgc acgacgacgg cacccagcgc
     1921 tcgctgcacg cgccggcggg catcgacgcc gcggagctgc tcccggacgg ccgtgcggtg
     1981 gtgtccgatg cgctccgcca cctccggatc tacggacctg gtagcaccgt gctcgccgat
     2041 ctcgagttgc cgacacgcgt catgtcgctt cggggagacc agactcgcct tgtggcaatt
     2101 cccagctacc tgagcgaggc atcacctcct ctgctgatcg acctcgagcg ctatcgcgtc
     2161 attgcacagc tcgcaggaca cgtcggccag gtattctccg cgcgctgggt cgcaggaggg
     2221 cgcatcctca ccgccggtgc cgacggcacg gcgcgcatgt gggacggagt gacaggccgg
     2281 ctgcttcagg tctatcgcgg gggctcacgc ttccttggcg acgtgaccct gatgaccgat
     2341 gacgtggtaa tcggcggtga cgccgatggc gttctgcggt tctgggacgc aacgagcggc
     2401 gccaagctat gggcgttgca ggcgcacaag tccgccatta tcggtgttca tgtcgagggc
     2461 aacggcgtcg ttacgcgcgg atttactggg gaaatctctc gatggaggtt gccggcggcc
     2521 gaggaagtga tcggcgcgtg cggtaaccat ccccactgcg ctatcgtcgc acaatgacga
     2581 aatcaccacg caagcttgtg ttgcataagg aaacccttcg cgccctgacc agcatggaac
     2641 tccgtcgagc catcggcggc gctgacggca acaatcaggt cgacaacaca tttaacaaga
     2701 actgtcctgc cgtgaacatg atcgtcgcgc cgggtcaatg atacccggcc agatacccgg
     2761 gagaccgggc gggtagcggc cactgcgagt tgctctgatt gcgctacggc gacatgggcc
     2821 tgttgtggtg agtgacagtc gtacgcatca agggtcgact cgcctcgacc gttgacgtga
     2881 cgacatcttc gtcgttaccg cccctcctgt ccacggattt gttcgcctgg tcgaccgggc
     2941 cgtccaggag tgaaaagccg accgagcatg ccaccgaccc cggccgcgcg gccggggccg
     3001 gcggtcgccg gcggtcggtt ttcatctcca ggatcgcaga gccgaccggc cccccccatt
     3061 gcccgcgccc tgcatgggcg tatcatgaag cgcgcgtgcg acgcatcatc ctgatcctgc
     3121 ttaccctggt cctcgcgccg tctgtggcga cggcgtcctg gtaccggtgc gcgtacgatg
     3181 ggacgatccg ttcggtctgc tgctgcccga cgaagccgga ccagcacaag aagaacgcac
     3241 cgccacccga cacgtcgctg cgtgctgcgt gctgctgtac ggtcatccag gccacgagca
     3301 acgctcgaga gatcgaagcc agtacaccgg ctcagagcaa gttggctccg atggcgctgg
     3361 ccgtgcccac cgtggtggcc cctcggccgg caccgatcct tgcggttgcg ctcgatcgtc
     3421 cgcgtgccca gggcgaccca cccgatacgc tgttcactcg ccgctgcttg cttctcctgt
     3481 gatcgcaggc tctcgacctc gtcgatagag cccgatctgg tcgcgcatcc gcgtggtcct
     3541 tccgctgcat tcggcggaga caggagaaca gatgtgtttc aagcacttga cccgattgtt
     3601 gccgggggtc ttcttcggcc tcgcgggctg cgtcgatgac gcacacgtgc gccagcacga
     3661 cgtcatgtat gcgaccgtat cccgtgtcgt cgccgagcgg gcgcgtaccg gcaggtcgaa
     3721 cgatgcatcg ctcgttgtgg atggcgcgct cgaccgcgcg gcggtcgtcg cggcggtgct
     3781 cgcgcacaac cccgacctgg acgctgtgcg ggcgacctgg cgcgctgcgg tggcggcgtt
     3841 cccgtcggcg atctcgctcg acgatccggt ggcgagctac gccctggcgc cgttcagcat
     3901 cggatcggat gcgcggttcg gccagcgtat cgagatcagc cagaagctgc cgtggccggg
     3961 caagcgagag ctgcgcggcg atgcggcgct cgcggatgcc gaagcggcgc aggcggactt
     4021 cgaatcgctc cagctcgacc tcgccgaggc tgccgtccag gcgttcgacg acgactacat
     4081 cgcgacgcgc gcgctcgaca tcaaccagca tcatcgcgag ctgctggagc gcatcgagcg
     4141 gagcgcgctc gcgcagtatg cggccggccg ggcatcgcag caggactcgc tcgaggcccg
     4201 cgctcacatc atcgagctcg accgcgagcg gctgatgctc gaaaggcagc ggcgggtcgc
     4261 cgtcgccacg ctgaaccggc tcttgcaccg caaggcggac gccgagctgc cgccgctccc
     4321 ggcgcgcctg gccgtctcgc ggtcgaccga gccggcacac gagcacccca agcagcttgc
     4381 tgccgcggcg cgaatccgag cgcgccaggc cgaagtcgac ggcgcggccc tcgcgttcta
     4441 cccggacttc gaggtgatgg gctcgtatga ctcgatgtgg gacacctggc agcaccgctg
     4501 gatgatcggc gtcggcatcg acatcccgct tcagcgcggg aagcgacgcg cggatctcga
     4561 gcgtgcgcgc gcagagcagg ccagggccga ggccgatctc gcctcggtga ccgacatgct
     4621 cgatgagcgc cgcgagcgag tgcgccgcga ggtggacgag tcgaccaggg cgctggagct
     4681 ctacgagcaa cagcttctgc cgaccacacg cgagcgcgtc gatgccgcgc tggcgggatt
     4741 caccagcgga cagaatccat tctccaccgt cgtcatggcg gagcacgagc tgcgcgacgt
     4801 cgagctggcg atcgagagag cgcgcgcgga tctcgatcgc cagctcgccg cactcgatcg
     4861 actcgaaggc cgtatccctg gaagtggacg atgaccgact cgaacgacaa gcccgtcccg
     4921 atgaacccgg aggcgggccc gacaccgacg gcgggtccag ccccgcacac aacccggcgt
     4981 gaacgagaca ggacacggat cgtgtcggcg gcggtgatcg gcgcgctgat cctcctcgcc
     5041 gtcgtgttcc ggcatcccct gatcgcctgg ttcactggct cgtcgagtgg cggtggatcg
     5101 acgtctgccg ccgcgcacgt cgcggctgcc gggctctcga ttgatacgtc gatcgagccg
     5161 gatccgcccc gcgagaatgg cgaccgtgca cacgtcctgg tgcgcgacgc acaggacaag
     5221 ccggtcagcg gggccaaggt ccgcctcgaa tacgacatgc ccgcgatggg gacgatgcaa
     5281 gcgatgcacg gtggcgtcga cgccagcgag gacggcccgg ggcgctacac gatcccgttc
     5341 gacctgggca tgggcgggag ctggacgatc tcgatccacg tgaagacggc atcggccacc
     5401 gcgaccgcac ggtacacgct gcgcgtcgga tcctcggggt tgaccgtgct cggcggcgag
     5461 tccggacgcg ccggccgcgg cgggtcgggc tcgatgggtg ggatgaacgg tacagacagc
     5521 ggcagcgaca tcagctatta cacgtgctcg atgcacccca cggtgcactc gaaggagccg
     5581 ggcaagtgtc cgatctgctc gatggacctc acgccggtca tgcgggacga ggagcaggcc
     5641 ggggtgatcc acatcgacga gaatcgccgc gcgctgctcg gaatccgcac caccaaggtc
     5701 gtgcgcagac cgatcgatct cgagatcacc gccaaggggc ggcttgccgt cgacgagacc
     5761 aggctccacg acgtcgtgct caaggtcggt ggctatgtca gcgacctgcg cgtgaacgcg
     5821 acaggccagt cggtcgcgcg cggacagacg ctgttcaccc tgtacagccc cgagctctac
     5881 gcggcggagc aggaatacct gatcgcgaac cagaaccgcg aggcgatgcg cgtgaacggc
     5941 gacgcgagcc acagcgagcg gctggcgcac gctgcagaga ccaagctgcg cctgtggggt
     6001 cttgacgatg accagatcgc cgagctcgcc aggcacggcg agccgatcga gcgtgtgccg
     6061 ttcaagtcac cggcgagcgg tgtggtggtc gagaagaacg tcgtggatgg cgcctcggtc
     6121 atggccggcc agcggctgtt ccgggttgcc gatctgacgg atatctgggt cgaggcggat
     6181 gtctacgagt ccgatctgcc gcggatcacc aagaacctgc ccgcatcgat cacgctcgac
     6241 tacctgccgg gcaagatgtt cgagggcaag gtcgcattca tttacccgta tctcgatccc
     6301 gcatcgcgga cggggcgcgt tcgcgtcgcg ctcgcgaatc aggggctcga gctcaagccg
     6361 gacatgtacg ccacggtgac gttcaagctt ccgctcggcc cgcgtctcgt cgtcccgatc
     6421 tccgccgtgg tctacaccgg cccgcgccgg atcgtgttcg tcgacctggg caacggggca
     6481 ctgcgcccgc aggaggtcac gatcggcgcg cgcagcggcg acctgatcga gatcacgacc
     6541 ggccttggcg agggcgatat cgtggtcagc tcgggcaact tcctggttgc ggcagagagc
     6601 cgcgtgcgct cggccggttc gttctgggag gacagccatg gcgaccagta atgaccagcc
     6661 cggcatcatc ggtcgtgtga tcgccgggag cgcaagcaac ccgctgatca cgatcatgct
     6721 cgtgatcggc ctcggggtgt gggggtggct gtcgctgagg cgcgcaccgc tcgatgcgat
     6781 cccagatctg tcggacgccc aggtgatcat cttcaccgag tggatgggcc gaagccccga
     6841 tctcgtcgag gatcagatca cgtatccgat ctcgtcgtcg ctgatcgcgg cgcccggcgt
     6901 gaagtacgtg cgcggccagt cgatgttcgg gatgtcgttc gtctatgtca tcttcgagga
     6961 cggcactgat atttactggg cgcgcagccg ggtactcgag tacctcaacc aggcgcaggc
     7021 gcagctgccg gcgggtgtga ccccgcggct cggccccgat gcgacgggtg tcggctgggt
     7081 gttcgagtac gcgctggtcg atcgcaccgg caagcacgat gcgcagcaac tgcgctcgct
     7141 gcaggactgg aatctccggt atgcgctcgc gagcgtcaag ggcgtggcgg agatcgcatc
     7201 agtcggaggc gcggtcaagc agtaccaggt caacgtcgac ccacagaagc tgctcgcgct
     7261 caaggtcccg cttcgcgacg tcatgatcgc gctccagcgc tcgaacgccg acgtcggcgg
     7321 tcaggtgatg gagatcgccg ggcacgagca cgtgattcgc gggcgcggct acgtgcacgc
     7381 ccggaccgat ctcgaggagg tgccgctcag gaccaccgat gcgggcgtcc ccgttcgcgt
     7441 caaggacatc ggcgaggtcg agatcggcca ggacatccgc cgcggactca ccgatctcga
     7501 cggcgagggc gaggtccccg gcggcatcgt gatcatgcgc tacggcgaga acgcgctcga
     7561 cgtgatcgac ggcgtgaaag cccggctcgc cgagattcag cgcacattgc ccgagggcgt
     7621 ccaggtcgtc gtgacctacg atcgctccgc gctgatccgc gcgtcgatcg acacgctcca
     7681 gcacacgctg atcgaggaga tgatcgtcgt ggcgctgatc atcttcctgt tcctgctcca
     7741 cgtgcgcagc gcggtgatcc cgatcctgac cctgccgctc ggggtcctgc tcgcgttcat
     7801 cccgatggcg taccagcacc tcactgccaa catcatgtcg ctcggcggca tcgccgtggc
     7861 gatcggcgcg atggtcgatg cctcgatcat catcatcgag aacatccaca agaagctcga
     7921 cgactgggag cgccacggca ggcccgggcc gcgcctgggc gcgatcaccg ccgcgatgca
     7981 ggaggtcggg ccgtcgatct tcttctcgct cctcgtgatc accgtgtcgt tcatcccggt
     8041 gttcacgctc cagggcaccg agggccgcct gttcaagccg ctcgcgttca ccaagaccta
     8101 ctcgatgggg tttgcggccg tgctcgccgt gacgctgacg cctgcgctcg ctgcgatctt
     8161 cgtacgcggc aagatcccgc gcgaggatcg ccacccagtg aatcgcctgc tcacgcgcgc
     8221 gtatgcgccc gtcgtgcgct gggccgttcg ctaccgcggc ctcgtgatcg cccttgccgc
     8281 gctggtgatc gcgttcacga tcccggcgtg gttccgtctg agcagcgagt tcatgccgcc
     8341 gctcaacgag ggcgcgatcc tctacatgcc gaccgcgcct ccgggaatgt cgatcgccga
     8401 ggcatcccac ctcatgcagg tgatggacaa ggagctcaag tcgttccccg aggtcgcctc
     8461 ggtgttcggc aagaacggcc gcgccgagac cccgacggat ccggcaccgt tcggcatggt
     8521 cgaggcaacg gtcgtgctga agccgcacga ccagtggcgc cccgggatga cctgggatcg
     8581 gctcgtcgcc gagatggacg ccaagctcca ctaccccggc atgccgaaca tcttctggat
     8641 gccgatccag acccgcaccg agatgctgtc caccggtatc cgcagcccgc tcgggatcga
     8701 ggtgttcggc gacaacaccg accagatcga gaaggcagcg gtcgcgatcg agagcgccgt
     8761 cgtcaacgtc cccgggacgc gcagcgcgtt cgccgatcgt tcgaccggcg ggttctacat
     8821 cgatctcgtg gtcaagcgcg ccgctgcggc gcggctcggg ctcaccgtgg gcgacgtcga
     8881 ggaggtcatc cagaccgcga ttggcgggat gaacgtgtcc gagaccgtcg atggccgcga
     8941 gcgctactcg atcaacctgc gctacgcgcg cgagtttcgc gatgacccgt cgatgctcga
     9001 gcgcgtcctc gtgccgacgc catccggtgc acaggtcccg ctgtcgcagg tcgccgaggt
     9061 caagaccgtg accggtccgc cgatgatccg ctccgaggac ggcaagctcg tcggctacgt
     9121 cttcctcgac accacgcggc cgatgtcaga ctacatcgcc gatgcgcagc gcgtcgttgc
     9181 cggcctccag ctgcccaagg gcgttcgcgt tgggtgggtc ggccagttcc agtacctcga
     9241 gcgcgccaag gccaggctca agatcgtcgt tccgatcacc ctgatgatcg tcttcttccc
     9301 gatcttcctg aacacgcgct cgctgatcga gaccggcatc gtgctgctcg cggtcccgtt
     9361 ctcgctggtc ggcgcgatct ggctgatcta tctgcttcac tacaacatca gcgtcgcggt
     9421 ctggatcggg atgatcgcgc tcgccggcct cgacgccgag accggcgtgg tgatgctgct
     9481 ctacctgacg ctggcacatc gcgcgtggaa ggccgagggt cggttgaaca cacccgacga
     9541 tctgcgcgag tcgatcgtcg agggcgcggc gcaccgcatc cggcccaagc tgatgaccgt
     9601 gctcacgatg atggtcggcc tcctgcccgt cctgtggagc agcggcaccg gggccgatgt
     9661 catgaagcgg atcaccgcac cgatggtcgg cggcctggtc acctcgttcg tgctcgagct
     9721 gctggtctac cctgcgatct tcgcgatgtg gaaggcgcgg gcttgacccg ggaccgccgg
     9781 cggagccgtc agcgcaggaa ctcggcgatc tgcttcgcga cgt
//
LOCUS       DASVOR010001130         2641 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_40853,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001130 DASVOR010000000
VERSION     DASVOR010001130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40853"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1377
                     /locus_tag="VF516_21580"
     CDS             <1..1377
                     /locus_tag="VF516_21580"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8110344.1"
                     /translation="MWYAVVFRPDHAPAWKAYLDLRAQGSERARAVLEEALDRLGLGA
                     AYPRLMREAGARDLLDELVYFSLDLAEDERARVKVYFRHYQATIGDLERVIAGAGGAE
                     PGDIGAFCTTVLGHSGPYRARPLVSCWAFTGGEPSGATLYAPIAYYVRDDAEARARIL
                     RWMDCVEIDPDGYERALAAFARRSLESGVGMHSYVSFKRDRGLPRVTAYLAPEAYRTF
                     PPASLARRETPAPRRPRSPEPLIHRYETVERLTDHPLFQRLAREPAAIAPLWAILANN
                     WVAVGDQFPRWLAHLVARVEHDGMRSILAKQLNDELGDGDPARAHRVLFQTMLADLEP
                     YAMPGDRDALLAPGRRFAQGLAHNYLERPWLEAVGGSLVAEVYGKQVDQLVGALLRRQ
                     RDLDPASLTWLVLHETLEEDHAGEAIDLARMTPPTAEARAAICRGAEELAALGLRYFD
                     DLHEVVFR"
     gene            1374..2567
                     /locus_tag="VF516_21585"
     CDS             1374..2567
                     /locus_tag="VF516_21585"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8110345.1"
                     /translation="MIAAYAPAAPNFAALRSRFPTLVERTYFATHCLGPVLDTALADL
                     EDYRRTILLRNRAIETWLARIDEIRGLFAQLVHADPDEIALGANATACHASLAAALDP
                     DLGADLGADLGAAPGRTRIVTTSLDFPSTRYLWHAQARRGFEVIEVGSPDGIAMPVDA
                     LVAAIDERVAIVAVSLVAYSNGALLRAREVIDAAHAAGAIVVLDAYQAVGIVPIDVRA
                     LGADAVIAGTHKWLHGASNGLAFAYVRRALAERLTPAYPGWFSHARTLDFDPVYTPAP
                     GARRFEQGSTSVEAVYAARAGVQFALDVGVEAIRARSLALSDHLIAGADALGIPLGTP
                     RDHAERAGVVCLGVRDPSQVTEQLRGRGIDVDTRPGTGIRLSLHPCNLRDEVDRVLAK
                     LRRHV"
BASE COUNT          324 a         1014 c          950 g          353 t
ORIGIN      
        1 atgtggtacg cggtcgtctt ccggccggat cacgccccgg cgtggaaggc ctacctcgac
       61 ctgcgggcgc agggcagcga gcgcgcgcgg gcggtgctgg aggaggcgct cgaccggctc
      121 ggcctcggcg ccgcctatcc ccggttgatg cgggaggccg gggcgcgcga tctgctcgac
      181 gagctcgtct acttctcgct cgacctcgcc gaggacgagc gtgcccgcgt caaggtgtat
      241 tttcgacact accaggcgac gatcggcgat ctcgagcgcg tgatcgccgg cgccggcggc
      301 gcggagccgg gcgacatcgg ggcgttctgc accaccgtgc tgggccacag cggcccgtac
      361 cgcgcgcgcc cgctggtcag ctgctgggcg ttcaccggcg gcgagccgtc cggcgcgacg
      421 ctgtacgcgc cgatcgccta ctacgtgcgc gacgacgccg aggcccgggc ccggatcctg
      481 cgctggatgg attgcgtcga gatcgacccc gacgggtacg agcgcgcgct ggcggcgttc
      541 gcgcgccgat cgctggagtc cggggtcggc atgcacagct acgtgtcgtt caagcgcgac
      601 cgcgggctgc cccgggtgac cgcgtacctt gcgcccgagg cctaccgcac gttcccgccg
      661 gcctcgctgg cccgccgcga gaccccggcg ccgcgccgtc cgcgcagccc cgagccgctg
      721 atccaccgct acgagaccgt cgagcgcctg accgatcacc cgctgttcca gcggctcgcg
      781 cgcgagccgg cggcgatcgc cccgctgtgg gcgatcctcg ccaacaactg ggtcgcggtc
      841 ggcgatcagt tcccgcgctg gctcgcccac ctggtcgcac gggtcgagca cgacggcatg
      901 cgcagcatcc tggccaagca gctcaacgac gagctcggcg acggcgatcc cgcccgggcc
      961 caccgcgtgc tgttccagac catgctcgcc gacctcgagc cgtacgcgat gcccggcgat
     1021 cgcgacgcgc tgctcgcccc gggccggcgg ttcgcccagg gcctcgcgca caactacctg
     1081 gagcggccgt ggctcgaggc ggtcgggggc tcgctggtcg ccgaggtcta cggcaagcag
     1141 gtcgaccagc tggtcggcgc gctgctgcgc cgccagcgcg acctcgatcc ggcgtcgctg
     1201 acctggctcg tgctgcacga gacgctcgag gaggaccacg ccggcgaggc gatcgacctc
     1261 gcgcgcatga ccccgccgac cgccgaggcg cgcgcggcga tctgccgcgg cgccgaggag
     1321 ctggccgcgc tcgggctgcg ctacttcgac gacctccacg aggtggtgtt ccgatgatcg
     1381 cggcgtacgc gccggcggcg ccgaacttcg ccgcgctccg gtcgcggttc cccacgctgg
     1441 tcgagcggac gtacttcgcg acccactgcc tgggcccggt gctcgatacc gccctggccg
     1501 acctcgagga ctaccggcgc acgatcctgt tgcgcaaccg cgcgatcgag acctggctcg
     1561 cgcggatcga cgagatccgc ggcctgttcg cgcagctcgt ccacgccgat cccgacgaga
     1621 tcgcgctggg cgccaacgcg acggcgtgcc acgcgagcct cgccgcggcg ctcgatcctg
     1681 acctgggcgc ggacctgggc gcggacctgg gggccgcgcc gggacggacg cggatcgtca
     1741 ccaccagcct cgacttcccg tcgacccgct acctgtggca cgcccaggcg cggcgcggct
     1801 tcgaggtgat cgaggtcggc tcgcccgacg gcatcgcgat gccggtcgac gcgctggtcg
     1861 ccgcgatcga cgagcgcgtc gcgatcgtcg cggtctcgct ggtcgcgtac agcaacggcg
     1921 cgctgctgcg cgcccgcgag gtgatcgacg ccgcgcacgc ggcgggcgcg atcgtcgtcc
     1981 tcgacgccta ccaggcggtc ggcatcgtcc cgatcgacgt ccgcgcgctg ggcgccgacg
     2041 ccgtgatcgc cggcacccac aagtggctgc acggcgcgag caacggcctg gcgttcgcgt
     2101 acgtccggcg cgcgctggcc gagcggctga cgccggcgta cccgggctgg ttcagccacg
     2161 cccgcacgct cgacttcgac ccggtctaca ccccggcgcc cggggcgcgc cggttcgagc
     2221 agggctcgac ctcggtcgag gcggtgtacg ccgcgcgcgc cggcgtgcag ttcgcgctcg
     2281 acgtcggcgt cgaggcgatc cgggcgcgca gcctggcgct gtccgatcac ctgatcgccg
     2341 gcgccgacgc cctcgggatc ccgctcggca cgccgcgcga tcacgccgag cgcgccgggg
     2401 tggtgtgcct cggtgtccgc gaccccagcc aagtcaccga gcagctgcgc gggcgcggga
     2461 tcgacgtcga cacccggccg gggaccggga tccggctgtc gctccatccg tgcaacctcc
     2521 gcgacgaggt cgaccgcgtg ctggccaagc tccgtcgcca cgtctagtca caacccggtc
     2581 acaaccggcc acagcccggc gcggccaggc ccaccgattt cgggtcagcg ggcgcggctc
     2641 g
//
LOCUS       DASVOR010001131         5656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_479137,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001131 DASVOR010000000
VERSION     DASVOR010001131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5656
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479137"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1484
                     /locus_tag="VF516_21590"
     CDS             <1..1484
                     /locus_tag="VF516_21590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEX8110346.1"
                     /translation="HAVDINHLTMAALDGSELSNKEKGRAKNFFALGLVFWMYGREPE
                     GEIRRIYQQFAKKPEFGEANVKVFKAGYHYGETAEVFQTVYKVPPAVFKPGMYRNITG
                     NEAIALGLVVGAELAGKRLFYAGYPITPASSILHSLAKYKNFGTMTFQAEDEIAAMGA
                     ALGAAYGGSIAVTASSGPGVALKGEAMGLGVMTELPVVILNIQRGGPSTGLPTKTEQS
                     DLSQSLFGRNGEAPIPVLAAKSPGDTFYCAIEAVKIATRYMVPVILLSDGYIANGSEP
                     WPLPNLATLPRFEVVHRTDPSGYFVYQRDPRTLARSWVIPGTPGLEHRIGGIEKDALT
                     GNISYAPDNHEKQVRVRAEKIERVAQDIGELDLSGAESGDVLLVGWGGTYGALRQAQQ
                     QLAAQGKQVSHAHLRWLSPLEPGLAKIIHNFKRVVVAELNMGQLRQVIRATFLIDAIG
                     LNKIQGQPFKVREVIDAIEKQLDTKDAKHTEPAQVATQPLASA"
     gene            1506..2543
                     /locus_tag="VF516_21595"
     CDS             1506..2543
                     /locus_tag="VF516_21595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEX8110347.1"
                     /translation="MTEAVKIKLSKKDLESDQDVRWCPGCGDYAILATVQRTLAQIGI
                     KRENTVFISGIGCSSRFPYYMNTYGFHTIHGRAPAIASGLKITRPELDVWVITGDGDG
                     LSIGGNHMIHALRRNMDLKILLFNNQIYGLTKGQYSPTSAVGTRAPSTPTGSIDHPLN
                     PISLALGAGATFVARAADTDAKLMGAILQEAYQHKGAVFIELLQNCPVFNDGVWEAVK
                     DDPAGRQIALVEGKPLVFAGGTKGIKLGPGMTPQVVDVGDGPGKTPLSEIMVHTERGS
                     MLYASLLAQLVQPEFPIPMGVLYRHDKPTYSELANQQIDDAVAKQGHGDLNKLMYSGM
                     VWEVGADGTRH"
     gene            2735..3358
                     /locus_tag="VF516_21600"
     CDS             2735..3358
                     /locus_tag="VF516_21600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX8110348.1"
                     /translation="MSAHVVSADEVERTITAFTRLDQRGDKKAMNKLAQRLQREQPFL
                     LQHAAAVKTEHGDAVGEAAVFYATLVWAMFDRGRTATLPRLTAQNLADADKIVTDALG
                     AVDGLADRPPHERVAPALVETQPHIYAKLATLLAEDVKEAAMTAETAATIVKPTQVVI
                     EAFDAALSGRRAGERQGTFVADTKVGRNEPCPCGSGKKYKKCHGEVA"
     gene            complement(3383..4252)
                     /locus_tag="VF516_21605"
     CDS             complement(3383..4252)
                     /locus_tag="VF516_21605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110349.1"
                     /translation="MISEANWKVHWRLTESAGIMIYLADYRGRRVLWEASLPYVTVDH
                     QRETLEVRDDPGEPHGPWWIPLGTRTLQGPVRMQSFRGGIELSAAFAAGPYHYTQLWR
                     FHDDGRLCPWLTIYGPGVHDQHTYHPHWRFDFDIDGARHDTVERFEDGRWQRVDREGW
                     FPYSGEADEHGNVWRQVERSETGAGDPDGSDGGAGGKAPRGSNVASRATIHIRPHTWD
                     DAELFAIRHHDGEWAPYSPRSASGAQTFPAAYVGEEPLDGEDVMLWYVAHVHFDQSFP
                     YTAGPWIKVEGFG"
     gene            complement(4261..4977)
                     /locus_tag="VF516_21610"
     CDS             complement(4261..4977)
                     /locus_tag="VF516_21610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110350.1"
                     /translation="MASSKKPVKTAKPAKPKPKSRPAAGGATRRARSTHTPGHTPGDT
                     RGGTPANAQADLGADIAKADPALFDPLTPGEVADALRTLTEDRRLSSMAKVGRYRVIC
                     SEPLVVKPPHWMAGHRLARIVAYDYAADRTVDACVDLDAGVVAHLEITRAQPMLSREE
                     EALAVSIAMVDGRVREKLALGDTAQSALHYWGRGPDDLAFARRSAAVVFGRADGHASV
                     VAIVDLLENMVTQVVPAERW"
     gene            5033..>5656
                     /locus_tag="VF516_21615"
     CDS             5033..>5656
                     /locus_tag="VF516_21615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110351.1"
                     /translation="MVTRRSLLASLLLSAACSAAYADQPVGPQLPPAHVALAIAPARV
                     RAPYDVQILRGNGETLPTYALRDRFYVQGNAGERYVIRITNPTAHRIEAVISVDGLDV
                     IDGENGDLRKRGYVVPAYGETRIEGFRTSQADVATFRFSSVSGSYAGQKGKPRNVGVI
                     AVALFEEQPAPDEQQIIVGQAPRPPHRPRPYDYRDDLDGRRMSPRGAG"
BASE COUNT          886 a         2033 c         1948 g          789 t
ORIGIN      
        1 tccacgcggt cgacatcaac cacctgacga tggccgcgct cgacggcagc gagctgtcga
       61 acaaggagaa gggccgcgcc aagaacttct tcgcgctcgg cctggtgttc tggatgtacg
      121 gccgcgagcc cgagggcgag atccggcgga tctaccagca gttcgccaag aagccggagt
      181 tcggcgaggc caacgtcaag gtgttcaagg ccggctacca ctacggcgag accgccgagg
      241 tgttccagac cgtctacaag gtcccgccgg cggtgttcaa gcccggcatg taccgcaaca
      301 tcaccggcaa cgaggcgatc gcgctcggcc tggtggtcgg cgccgagctc gccggcaagc
      361 ggctgttcta cgccggctac ccgatcacgc cggcgtcgtc gatcctgcac tcgctcgcca
      421 agtacaagaa cttcggcacg atgacgttcc aggccgagga cgagatcgcc gcgatgggcg
      481 cggcgctcgg cgcggcctac ggcggctcga tcgcggtgac cgcgtcgtcg ggcccgggcg
      541 tcgcgctcaa gggcgaggcg atgggcctgg gcgtgatgac cgagctgccg gtcgtgatcc
      601 tcaacatcca gcgcggcggg ccgtcgaccg gcctgccgac caagaccgag cagtccgacc
      661 tctcgcagtc gctgttcggc cgcaacggcg aggcgccgat cccggtgctc gccgccaagt
      721 cgccgggcga cacgttctac tgcgcgatcg aggccgtgaa gatcgcgacc cgctacatgg
      781 tgccggtgat cctgctcagc gacggctaca tcgccaacgg cagcgagccg tggccgctgc
      841 ccaacctcgc caccctgccg cggttcgagg tcgtgcaccg caccgatccg agcggctact
      901 tcgtctacca gcgcgatccg cgcacgctcg cccgctcgtg ggtgatcccc gggacgcccg
      961 gcctcgagca ccggatcggc ggcatcgaga aggacgcgct gaccggcaac atctcgtacg
     1021 cgccggacaa ccacgagaag caggtccggg tccgcgccga gaagatcgag cgggtcgccc
     1081 aggacatcgg cgagctcgat ctgtcgggcg ccgaatccgg cgacgtgctg ctcgtcggct
     1141 ggggcggcac ctacggcgcg ctccgccagg cccagcagca gctcgccgcg cagggcaagc
     1201 aggtcagcca cgcgcacctg cgctggctgt cgccgctcga gcccgggctc gccaagatca
     1261 tccacaactt caagcgcgtc gtcgtcgcgg agctcaacat gggccagctc cgtcaggtga
     1321 tccgcgcgac cttcctgatc gacgcgatcg gcctcaacaa gatccagggc cagccgttca
     1381 aggtccgcga ggtcatcgac gcgatcgaga agcagctcga caccaaggac gccaagcaca
     1441 cggagcccgc ccaggtggcc acacagccgc tggcgagcgc ctagcgaggc gaaggaaaat
     1501 cagtcatgac cgaagccgtc aagatcaagc tctccaagaa ggacctggag tccgatcagg
     1561 acgtccggtg gtgcccgggg tgcggcgact acgcgatcct cgccaccgtc cagcgcacgc
     1621 tggcccagat cggcatcaag cgcgagaaca ccgtgttcat cagcgggatc ggttgttcgt
     1681 cgcggttccc gtactacatg aacacgtatg gcttccacac gatccacggc cgcgcgccgg
     1741 cgatcgcgtc ggggctcaag atcacgcggc ccgagctcga cgtctgggtg atcaccggcg
     1801 acggtgacgg cctgtcgatc ggcggcaacc acatgatcca cgcgctgcgg cgcaacatgg
     1861 atctcaagat cctgctgttc aacaaccaga tctacggcct gaccaagggc cagtactcgc
     1921 cgacctcggc ggtcggcacc cgcgcgccgt cgacgccgac cggctcgatc gaccacccgc
     1981 tcaacccgat ctcgctcgcg ctcggcgccg gggcgacgtt cgtggcgcgc gcggccgaca
     2041 ccgacgccaa gctgatgggc gcgatcctgc aggaggccta ccagcacaag ggcgcggtgt
     2101 tcatcgagct gctccagaac tgcccggtgt tcaacgacgg cgtgtgggag gcggtcaagg
     2161 acgacccggc gggccggcag atcgcgctgg tcgagggcaa gccgctggtg ttcgccggcg
     2221 gcaccaaggg catcaagctc ggcccgggca tgaccccgca ggtcgtcgac gtcggcgacg
     2281 gcccgggcaa gacgccgctg tccgagatca tggtccacac cgagcgcggc tcgatgctgt
     2341 acgccagcct gctcgcccag ctcgtgcagc ccgagttccc gatcccgatg ggcgtcctgt
     2401 accgccacga caagccgacc tacagcgagc tcgcgaacca gcagatcgac gacgccgtcg
     2461 ccaagcaggg ccacggcgat ctgaacaagc tgatgtactc gggcatggtg tgggaagtcg
     2521 gcgcggacgg gacgcggcac tgattgatcg agagggggcc ttcccgcccc ctctggaccg
     2581 gcagagccgg tccattcacc cctccgcgcg tcgcgtcgcg cggccctgca cctgcgcggt
     2641 ccgcgctcgt cgatctcttc gtcgaatgcg gtactcatcg tatgcgcgcg tgaggcgctt
     2701 gacgcggcgg ccaggccggg gaagattccg ccccatgtcc gcgcatgtag tctccgccga
     2761 cgaggtcgaa cgcaccatca ccgctttcac ccggctcgat cagcgcggcg acaagaaggc
     2821 gatgaacaag ctggcgcagc ggctgcagcg cgagcagccg ttcctgctcc agcacgccgc
     2881 ggcggtcaag accgagcacg gcgacgcggt cggcgaggcc gcggtgttct acgcgaccct
     2941 ggtgtgggcg atgttcgacc gcggccgcac cgccacgctg ccgcggctga ccgcccagaa
     3001 cctcgccgac gccgacaaga tcgtgaccga cgcgctgggc gcggtcgacg gcctggccga
     3061 caggccgccg cacgagcggg tcgcgccggc cctggtcgag acccagccgc acatctacgc
     3121 caagctcgcc acgctgctcg ccgaggacgt caaggaagcc gcgatgacgg cggagaccgc
     3181 ggcgacgatc gtcaagccga cccaggtcgt gatcgaggcg ttcgacgccg cgctgtcggg
     3241 ccgccgggcg ggagagcggc agggcacgtt cgtcgccgac accaaggtcg gccgcaacga
     3301 gccgtgcccg tgcggctcgg gcaagaagta caagaagtgc cacggcgagg tggcgtgacc
     3361 cggcgagtgc ccgccgcggc gatcagccga agccctcgac cttgatccac gggccggcgg
     3421 tgtaggggaa cgattgatcg aagtggacgt gggcgacgta ccacagcatc acgtcctcgc
     3481 cgtcgagcgg ctcctcgccg acgtaggccg ccgggaaggt ctgcgcaccg gacgcgctgc
     3541 gcggcgagta cggcgcccac tcgccgtcgt ggtggcggat cgcgaacagc tcggcgtcgt
     3601 cccaggtgtg cggccggatg tggatcgtcg cgcgcgacgc cacgttgctc ccacgggggg
     3661 ctttgccccc ggccccgccg tcggagccgt cggggtcccc agcccccgtc tcgctgcgct
     3721 cgacctggcg ccacacgttg ccgtgctcgt cggcctcgcc gctgtacggg aaccagccct
     3781 cgcgatcgac gcgctgccac cggccgtcct cgaaccgctc gaccgtgtcg tggcgcgcgc
     3841 cgtcgatgtc gaagtcgaac cgccagtgcg gatggtaggt gtgctggtcg tggacgccgg
     3901 ggccgtagat cgtgagccat gggcacagcc ggccgtcgtc gtggaaccgc cacagctggg
     3961 tgtagtggta cggcccggcg gcgaacgccg ccgacagctc gatgccgccg cggaacgact
     4021 gcatgcggac cgggccctgc agcgtgcggg tgccgagcgg gatccaccac ggcccgtggg
     4081 gctcgccggg gtcgtcgcgg acctccagcg tctcgcgctg gtggtcgacc gtgacgtacg
     4141 gcagcgaggc ctcccacagc acgcgccggc cgcggtagtc cgcgaggtag atcatgatcc
     4201 cggccgactc ggtcagccgc cagtgcacct tccagttcgc ctcgctgatc acgttgctca
     4261 tcaccagcgc tccgccggga ccacctgggt caccatgttc tcgagcagat cgacgatcgc
     4321 gaccaccgag gcgtggccgt cggcgcgccc gaacaccacc gccgccgagc gccgggcgaa
     4381 cgccaggtcg tcggggccgc gcccccagta gtgcagcgcc gactgcgcgg tgtcacccag
     4441 cgcgagcttc tcgcgcaccc ggccatcgac catcgcgatc gacaccgcga gcgcctcctc
     4501 ctcgcgcgac agcatcggct gggcgcgcgt gatctcgagg tgggcgacca cgcccgcgtc
     4561 gaggtcgacg cacgcgtcga ccgtgcggtc ggcggcgtag tcgtacgcga cgatccgcgc
     4621 cagccggtgg ccggccatcc agtgcggcgg cttgaccacc agcggctcgc tgcagatcac
     4681 ccggtagcgg ccgaccttgg ccatcgagct cagccggcgg tcctcggtga gggtgcggag
     4741 cgcgtcggcg acctcgcccg gggtcagcgg atcgaacagc gcggggtcgg cctttgcgat
     4801 gtccgccccg aggtcggcct gggcgttggc cggcgtgccg ccacgagtgt cgccaggggt
     4861 gtggccaggg gtgtgggtag acctcgcacg gcgcgtcgcc ccgccggcgg ctggcctgga
     4921 tttaggcttt ggcttcgctg gcttggctgt cttcactggc ttcttcgacg acgccatgcg
     4981 cggactatac acggctttag atcggcgacc gggatcgggc gtctcaaagc gcatggtgac
     5041 tcgccgctcg ctgcttgcct cgcttctcct gtcggccgcg tgctccgcgg cctacgccga
     5101 tcagccggtc ggtccgcaat tgccgccggc gcacgtcgcg ctcgcgatcg cgcccgcccg
     5161 ggtccgtgcg ccgtacgacg tgcagatcct gcgcggcaac ggcgagacgc tgccgaccta
     5221 cgcgctgcgc gatcggttct acgtccaggg caacgccggc gagcgctacg tcatccggat
     5281 caccaacccg acggcgcacc ggatcgaggc cgtgatctcg gtcgacgggc tcgacgtgat
     5341 cgacggcgag aacggcgacc tgcgcaagcg cggctacgtc gtgccggcct acggcgagac
     5401 ccggatcgag ggcttccgca cctcgcaggc cgacgtcgcg accttccggt tctcgtcggt
     5461 gtccggctcg tacgccggcc agaagggcaa gccgcgcaac gtcggcgtga tcgcggtcgc
     5521 gctgttcgag gagcagccgg cgcccgacga gcagcagatc atcgtcggcc aggcgccgcg
     5581 gccgccgcac cgcccccgcc cgtacgacta ccgcgacgac ctcgacggca ggcgcatgtc
     5641 gccgcgcggc gccggg
//
LOCUS       DASVOR010001132         1556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_99727,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001132 DASVOR010000000
VERSION     DASVOR010001132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99727"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1556
                     /gene="infB"
                     /locus_tag="VF516_21620"
     CDS             <1..>1556
                     /gene="infB"
                     /locus_tag="VF516_21620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEX8110352.1"
                     /translation="DNGEHAQPPARDPSRPAVGSVISLPPRIKITERTPASGRPPPGP
                     QPVNPIRGRFAQQQQQRGGPRGPGGRPGPHNDFGRKKLPLGKKGKQTTITTPAEHKRV
                     IRIEDSITVADLARNMGIKAPEVLKKLWGMGMTGVNINAAIDFDTAQILASEFGYEVQ
                     NVAFKEEDVFSQKEDDSEQLVPRAPVVTVMGHVDHGKTTLLDSIRKSRVAAGEAGGIT
                     QHVAAYKVSAGGHGDIVFLDTPGHEAFTEMRARGAQATDIVVLVVAANDGVMPQTLEA
                     LSHAKDAGVKIIVAVNKVDMPDAQPDRVRQQLADHSLIPEEWGGDTIYANVSALKGDG
                     IDNLLAQIAATAEFLELKANPDKPAAGLVIEARLDRNRGPMATLLIQEGTLRVGDILV
                     AGRTFGKVRAMLDDRGNTLEEAGPSTPIEVLGLDGVPEAGDQVNAAEDDKVAKQVVEH
                     RRQQERKRELSSKQRFSLENLMERNAEGASKELKVVLKADVQGSAEALKASLSKLSTD
                     KVRVNVIAAA"
BASE COUNT          280 a          556 c          538 g          182 t
ORIGIN      
        1 ccgacaacgg cgagcatgcc cagccgcccg cgcgcgatcc gtcgcgcccc gcggtcggca
       61 gcgtgatctc gctgccgccg cggatcaaga tcaccgagcg caccccggcc agcggccgcc
      121 cgccgccggg accgcagccg gtcaacccga tccgcgggcg gttcgcgcag cagcagcagc
      181 agcgcggcgg cccgcgcggt ccgggcggcc ggccgggccc gcacaacgat ttcggccgca
      241 agaagctgcc gctcggcaag aagggcaagc agaccacgat caccacgccc gccgagcaca
      301 agcgggtgat ccggatcgag gactcgatca cggtcgccga cctcgcccgc aacatgggca
      361 tcaaggcgcc cgaggtgctc aagaagctgt ggggcatggg catgaccggc gtcaacatca
      421 acgccgcgat cgacttcgac accgcccaga tcctcgccag cgagttcggt tacgaggtgc
      481 agaacgtggc gttcaaggaa gaggacgtgt tctcccagaa ggaagatgac tccgagcagc
      541 tggtgccgcg cgcgccggtc gtgaccgtga tgggccacgt cgaccacggc aagaccacgc
      601 tgctcgactc gatccgcaag tcgcgggtcg cggcgggcga ggccggcggc atcacccagc
      661 acgtcgccgc gtacaaggtc agcgcgggcg gccacggcga catcgtgttc ctcgacacgc
      721 cgggccacga ggcgttcacc gagatgcgcg cccgcggcgc ccaggccacc gacatcgtcg
      781 tgctggtggt cgcggccaac gacggcgtga tgccccagac gctcgaggcg ctgtcgcacg
      841 ccaaggacgc cggggtgaag atcatcgtcg cggtcaacaa ggtcgacatg ccggacgcgc
      901 agcccgaccg ggtgcgccag cagctcgccg accacagcct gatccccgag gagtggggcg
      961 gcgatacgat ctacgccaac gtgtcggcgc tgaaaggcga cggcatcgac aacctgctcg
     1021 cccagatcgc ggccaccgcc gagttcctcg agctcaaggc caacccggac aagccggccg
     1081 ccggcctggt gatcgaggct cgcctcgatc gcaaccgcgg tccgatggcg accctcctga
     1141 tccaggaggg cacgctgcgg gtcggcgata tcctcgtcgc cggtcgcacg ttcggcaagg
     1201 tccgcgccat gctcgacgac cgcggcaaca cgctcgagga ggccgggccg tcgaccccga
     1261 tcgaggtgct cggtctcgac ggcgtgcccg aggccggcga tcaggtcaac gcggccgagg
     1321 acgacaaggt cgccaagcag gtcgtcgagc accgccgcca gcaggagcgc aagcgcgagc
     1381 tgtcgtccaa gcagcggttc tcgctcgaga atctgatgga gcgcaacgcc gagggcgcgt
     1441 ccaaggagct caaggtcgtg ctcaaggccg acgtccaggg ctcggccgag gcgctcaagg
     1501 cctcgctgtc caagctgtcg accgacaagg tccgggtcaa cgtgatcgcg gcggcg
//
LOCUS       DASVOR010001133         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_216139,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001133 DASVOR010000000
VERSION     DASVOR010001133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216139"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="VF516_21625"
     CDS             complement(<1..1241)
                     /locus_tag="VF516_21625"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8110353.1"
                     /translation="MSGVGVVGRDAELGSVVGFLDRAAAAPTALVLEGEPGIGKSTLW
                     LAAVAAARRRGLTVLSSRPAESEHGLAYAGLGDLFDPILDVVIGELSPPRRRALEVAL
                     LRDEPTDEAADRRALAVAVRDVLRLLSQRKPVVVAIDDVHWLDASSASALAFALRRLD
                     GVVRLVVTRRLTGSALEHAVDVELLRIGPVSVGALHRILQDRFGRPFARQTLLRIHEQ
                     SGGNPLFALELARVLDDDVDPLEPLPVPATLEELVRTRLDDLPAATRDALAFVAALGT
                     ASESLLNGIGATPGALDPALAAQVVERERGAIRFTHPLLSSVLYNELGARRRVVHGRI
                     AAFVSDPLLRARHLALATDEPDPAVAAALDDAALIAGGRGASASAAELAEAALRLTPP
                     GDVGGRHRRALAAARAHRAAG"
BASE COUNT          268 a          627 c          562 g          163 t
ORIGIN      
        1 tccccggcgg cgcggtgggc gcgagccgcg gccaacgccc gtcggtggcg cccgccgaca
       61 tcgcccggcg gcgtcagccg cagcgccgcc tccgccagct cggccgcgct cgccgacgcg
      121 ccacgaccgc cggcgatcaa cgccgcatcg tccaatgcgg cggcgacagc cgggtcgggc
      181 tcgtccgtcg cgagcgcgag atgacgggcg cgcagcagtg gatccgagac gaaggcagcg
      241 atgcgtccgt gcaccacccg gcgccgcgcc ccgagctcgt tgtagagcac tgacgagagc
      301 aatggatgcg tgaaccggat cgcaccgcgc tcgcgctcca ccacctgcgc tgcaagcgcg
      361 gggtcgagcg cacccggcgt cgcgccgatg ccgttcagga gcgactccga tgcagttccc
      421 aacgccgcca cgaacgcgag cgcgtcgcgg gtcgccgccg ggaggtcatc gagacgggta
      481 cggaccagct cctccagcgt cgccgggacc ggcagcggct cgagcgggtc gacgtcgtcg
      541 tcgagcacgc gggcgagctc gagcgcgaag agcgggttcc cgcccgactg ctcgtgtatg
      601 cgcagcagcg tctggcgggc gaacggccgg ccgaagcggt cctggaggat cctgtgcaga
      661 gcacccacac tgaccggtcc gatccggagc agctcgacgt ccaccgcgtg ctcgagcgcg
      721 gaccccgtca ggcggcgggt cacgaccagc cggacgacgc cgtcgagccg gcgcagcgcg
      781 aaagccaacg cgctcgccga cgaggcgtcg agccagtgca cgtcgtcgat cgcgacgacg
      841 accggcttcc gttggctcaa cagtctgagc acatcgcgta cggccacggc gagcgcacga
      901 cggtcggcgg cttcgtccgt gggctcgtca cgcaggagcg cgacctccag cgcacgcctg
      961 cgcggcgggg acagctcacc gatcaccacg tcgaggatgg ggtcgaacag gtctccgagc
     1021 cctgcgtagg cgagcccgtg ctcggactcg gccggacgcg aggagagcac ggtcaacccc
     1081 cgccggcgcg ccgccgcgac agcggcgagc cacagcgtcg acttcccgat cccgggctcg
     1141 ccctcgagca ccaacgccgt cggcgcggcg gcggcgcggt cgaggaagcc aacgacggag
     1201 ccgagctccg catcgcgccc gacgaccccg acgccgctca cgcaccaatc ctgacagcgc
     1261 cacgaaccga agtcagcagc cccgaaacgg aacgccggtc accggtgcag gcccccgccc
     1321 cacaacccga acccgaaccg cccggaacgc cggtcaccag cggaggcccc tgccccacaa
     1381 cccaaaccgg aaccgaccgg aacgcggggc ccgcgggggt cgcccccgcg gccaacatcc
     1441 ggaacacccc ccgtccgcgc tctcggcgga cagggggtgc ccaggtgtgg acctcgtgct
     1501 ccgctattca aaccaggacg accggatcgc accgctcgcc gacgtcatgc agcgggttag
     1561 tgaccgcgag caggtgggct acggcaccgt cgcgccgcca tcggaaccgc gaaggccgcc
//
LOCUS       DASVOR010001134        14398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_391022,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001134 DASVOR010000000
VERSION     DASVOR010001134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14398
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391022"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..1498
                     /gene="atpD"
                     /locus_tag="VF516_21630"
     CDS             44..1498
                     /gene="atpD"
                     /locus_tag="VF516_21630"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEX8110354.1"
                     /translation="MATTDYSPNSIGRVVQVIGPVVDVAFDSAELPEINTALLVTNPS
                     IDKRDDNLTIEVAQHLGEHMVRCVAMDSTDGLVRGQAVKNTSAPISMPVGPEVLGRIL
                     NVVGEPVDEAGPVKVAQRSPIHRPAPKFTDQSVNVEMFETGIKVIDLLAPYRRGGKIG
                     LFGGAGVGKTVLIQELINNVAKKSGSYSVFAGVGERTREGNDLFHELGSAILFDGSTV
                     LSKTALVFGQMNEPPGARQRVGLSALTVAEYFRDVMKQDLLLFIDNIFRFTQAGSEVS
                     ALLGRIPSAVGYQPTLSTEMGGLQERITSTKDGSITSVQAIYVPADDLTDPAPATAFA
                     HLDATTVLNRAITEKGIYPAVDPLDSTSTILTPAVVGERHYQVAREVQRILQRYKDLQ
                     DIIAILGMDELSEDDKLTVSRARKIEKFMGQPFHVAETFTGMSGIFCSREDTVRSFEE
                     VLQGKCDDLPEQAFEMTGTIDDVRAKAAELAKRA"
     gene            1514..1930
                     /gene="atpC"
                     /locus_tag="VF516_21635"
     CDS             1514..1930
                     /gene="atpC"
                     /locus_tag="VF516_21635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEX8110355.1"
                     /translation="MASATTLGVNLVTPRGVVAHTDADSVQAPGELGEFELLPGHVPM
                     LAALKPGVLTIGTKARQRFAVSSGYLRVDLTGAVEILVEQAMPTVEIDAEAAKKDLAA
                     AEAELAKWGDRPLDGDYVNMQHRAGWARARLDAVAQ"
     gene            2315..3286
                     /locus_tag="VF516_21640"
     CDS             2315..3286
                     /locus_tag="VF516_21640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110356.1"
                     /translation="MGALDTDKSDQCLKNQPGGWTASQPDACIVVGDTITVMSPSVTG
                     SRPLVLVAQTQINVTGLLDVASHRDTGAVGAGAASSADCKPFGSSPLMGPPGGAGAGG
                     GFMFPGGDGGPGNGAGIDSVAGGQAAGVLVGAPTRLRGGCAGQIGGGGKPRDGGAGGG
                     AVYLVSAGEIRIETMGKIDVSGAGGAGVDMLAGGGGGGSGGMIVLYAPSITAMPGAIL
                     IANGGGGGGGSANSGPTMKGGDGHDPAVVSPIVAALGGTGGVLISANGGDGGSGFPAI
                     TSSNPSLKGGTGDIGGGGGGGGGGGGYLRSNKGLGSAAVSPTVDVVP"
     gene            complement(3318..7901)
                     /locus_tag="VF516_21645"
     CDS             complement(3318..7901)
                     /locus_tag="VF516_21645"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8110357.1"
                     /translation="MSPHESTEGTDRDVFVLHADEDTPFVQGELLPMLGLPDDRVILS
                     SELPFPGFIEHAIAASVRRSRLTLAILSPAYLRDRWVGFAELLSRNVRDDGRGGSLVP
                     LLLADCDVPEVLAQHAMLDCRTPAQRAASTARIRERLGRPDPVLGPIACPYPGMQPFT
                     AATASQFYGRTDEISKLLGLLDAGDPSIYVIGPSGSGKSSLIAAGLIPQLARKSRHRF
                     AVRKIRPGESPMRQLAEALACDPASVGGAVAALLEREACERLLVFVDQLEELFAQADA
                     DERHRFVQALGVLRADNRCHQVMALRADFYGALMSSELWPDIEGRFSRLEVAALRGES
                     LREAIVTPAREAGVYLERELVERLIADAAAEPGALPLLQEALVLLWGDGGHRLLRLAS
                     YDALGEAGRSGLAVALANRADAVLHRLSLPQQALARRIFLRLVSFGEGRPDTRRQQSR
                     AALAASGQESAHVDAVIDRLIDARMLTSDASAGGEPLVDLAHEALIGGWPEFQRWLLA
                     RRDDEQRRRLLELRAAEWTKRGRGGVGLFDAVELGEAERWIAGDTARELGYSDNLLRF
                     LAASRAAIVEAERRDQRAREDRERERSIAEHERMRLRRRLRRFATGALIVFSITATVL
                     GLLAWQQRQESERERLEAEHQLALNERDQARSLIVESGDRAAAIPHLAEAVRLGMNDP
                     ELRDLLADVNSGLWRITLGHGQPLTAVSFSPDGEQIVTASGNGTARIWDTATGTLVSG
                     SLAHDKEVWSAAFSPDGSLVVTASEDGTARVWNAATGSLFREPFKHDAKVWSAVFSPD
                     GKRIVTACGDHTARVWDVASAAPIGAPLAHASEVLSASFSPDGTLIASASRDGTARIW
                     NAGTGRPIGLPLKHEQAVHAAVFSPDGARIVTASEDGTARIWDAATGMPIGAPLRHEK
                     AVHSAVFSPDGTRIVTASGDGTARIWDAATGTPIGAPLQHERAVWSAAFSPTGTRVLT
                     TSLDGTARVWYATNGAPFSVPLPHDGPVRAAAFHPSGDFVVTASWDGTARVWDITTDT
                     AHRAAHERKRAVSSPISSPDGTRLVTLIRNDSAKNGTARIWDSATGAPTSIAITDPQG
                     VHHLAFNPDGTRFVTASGAIVRVWNAATGEPVTPRLITCPPPQDCNSTEALLEVAMSS
                     QFGFSNNSITRLRFSADGARVIAYASRVNGPMWGAKWDATTGSLITTRANVSGFSFGV
                     EDSVFESGESDVSNRSDRDERLTDPWLSALDGASLSTWAAQAARCGPSGDAVGPHRLC
                     PISEHAATPGEELERVRSVIALGDVAMFSSVWSLPRARYQQARSLLDPGIAQQSTAQQ
                     RGGQELQRIIALRLAILDALAGDLAHARPRLGADAPPTDALLLDRLGAVAHDDLQNPG
                     IALRLLQRAHALDPRDAGVLAHLAELYFVTRNHDPFTRCLADLDKLPTSADLQVAVAA
                     LAWADARLARLPETGHRSRLVGAYHQLADGARIGRTWKGTAHALGYGRFQPGDVKPVL
                     DLLAFLEHPVTDVTRATFADLLELPTSSRHRAR"
     gene            complement(7988..9850)
                     /locus_tag="VF516_21650"
     CDS             complement(7988..9850)
                     /locus_tag="VF516_21650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110358.1"
                     /translation="MRALGLLGVVLVLGAGCASASMAKEEGPTQITVAITSPAPGAEL
                     VAGGHATIAVTGTVATTTPARGALEAWVNGTRVDVKHGAFTAEVAAEPGVNHIKVEGG
                     DGIVALVSQELDVMWVAGYLPPRAGQTGFDLPAALELQLGQRFFDARLLGTALDRSTN
                     PVVARDVASVLELILWNVNLAGLLPPDGIVAGQGDTSINIKIPSITPSDIVVDARVID
                     GPQPAIALNIDLIGVFLAMTGTVQIVGQRLVVAGGLTADFHAAAQLTLGTGADGAIAV
                     TVTGATAGVGALTPNFTGDNGQQLDGLITLGSSDFRALIDGLLAGPIPAFINQLPPLL
                     QSLLGAADQLLANLSFTLDPGLGSPVMLQLGGHFGGLDIAAGATSGHVTVREDLSVQI
                     PGVQPIHPASLGAPRLDTSASAPVLDTSGLHLGIHLDFLNALLHALWNAGLLEGQLTS
                     AGLTAHVSAKLPPLVRPTPASSACKIDGERCDVQLQLGQVQIELPSFEQSFVIEAAAG
                     ARIKITGNTVSLAIQMTPDLRVWETSAMPGGPLTPDAVSHLISGVVWPQLFGAIGSNL
                     TFQLPLPDLASLGLGDLAPALANAQLSLQAGPRPTVTPSQLVLGADLVLATPAH"
     gene            complement(9850..12459)
                     /locus_tag="VF516_21655"
     CDS             complement(9850..12459)
                     /locus_tag="VF516_21655"
                     /inference="COORDINATES: protein motif:HMM:NF020700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110359.1"
                     /translation="MNHACVLGLVLVVTASCGDNHRPGLVVETRVARQTIAAGERVEA
                     RCAVIDGATGGPALDKAGNPLTDSVELDIAYEDTDAFARDSEDQVIAARAGMALVRCS
                     APSLGLVADAVDIEIVAGPPSRVITQLANPVAVAGEPVGATCLAFDAFDNPVKGAPQA
                     FAMSPLAPGVATTAESVTATAAGMYQVSCVISGAADVAEDFLAVVPGLPSALVAVVNP
                     ERPLYAVNDQVTLVAEAHDRFGNRVDDVALSYGSTPGLPSPSEAQFQFTQDGTFTLSA
                     AVTSPTQDGKTLTTTRTVLVNSSGPAITCMRADAPTQPSDAYMLQAAPSTIVVPVKIS
                     SAFSVQSVTINGAAATVNPATGNYQAGVPVDFGMNFVDVVATDTLGKQNSTTCTVLTA
                     QSFTAEDKSLGGAIGMRLDPNAIGDPQPTGLNSINDILHTVLSSTQLRSLVDSGLRAA
                     NPISSGRCGILACNPNVTYNANTVAWDQPSSTLALVPGGLQASVTLPNVRLSVNACGT
                     TCCIGGSTISVRATAIAATVSFSLQLQGGVLRAGLAASPTVTVGNVSLDGSGFCGFLV
                     NLLQSFFTGTVKDAVHNALTSFINSSVAPLLDQLVSSLDINTFGKSFMVPRLDGGGGI
                     PLQFGLAFSSFDITTSRALLGIGTQFTAATVAHNRPSLGIPGRTTTALLDPPGTTAAA
                     PVGLSLYEGVLNQVLHGLWRGGFFQATLQLAGGTATIDAVLPPVAAIAGNQAQLMLGG
                     IQATIRVPGFIDTPIPIEFGGRATASVTLSGDALHFGNLTLSQLFVSFQASLTQSQRT
                     AMGGLLTQVLQSVLVDAINGGLPAFPIPTFALPPSATGFGLPAGAQLGIFQPQLSTSG
                     THYVLTGAFGVRN"
     gene            complement(12591..13445)
                     /locus_tag="VF516_21660"
     CDS             complement(12591..13445)
                     /locus_tag="VF516_21660"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8110360.1"
                     /translation="MASDAAHAEAELPDIDDRLVEPGTPYEMLDGELVYVSPADPPHA
                     ELHVQLAALIEAHTGPAFQAGADLLTRTSKVDDIAPDISVYPAAPHPETGRRQLEQLA
                     FEIISKGSRRRAARKAAKLTQRGVRRVFMIDVKRSRVLEWSTALDGWRELDASGHIED
                     PALEVPLPIEAMIHAAKADDAIARALLARHNPVIEARLAERLDEGVREGLARGVEEGL
                     ARGVEQAARTLADAVLAVLAARGIAVGDAERARIVDEHDVGQLNRWLVRAATCADAHE
                     LWATTGGV"
     gene            13800..14048
                     /locus_tag="VF516_21665"
     CDS             13800..14048
                     /locus_tag="VF516_21665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110361.1"
                     /translation="MPKHRNPRPKVPKRPPALNPAHNLTHGDLIIRAQTAKLLWRVAH
                     AARATLWAPETEDGGEAVYAVLDDAIDDLDAHIRSGCA"
BASE COUNT         2136 a         4914 c         5228 g         2120 t
ORIGIN      
        1 tggagatcat cggcggcgcc gaggcactca aggggtaacg aacatggcta ctaccgacta
       61 ttcccccaac tcgatcggcc gcgtggtcca ggtcatcggc ccggtggtcg acgtcgcgtt
      121 cgattcggcc gagctgcccg agatcaacac ggcgctgctc gtgaccaacc cgtcgatcga
      181 caagcgcgac gacaacctga cgatcgaggt cgcccagcac ctcggcgagc acatggtccg
      241 ctgcgtcgcg atggactcga ccgacggcct ggtccgcggc caggcggtca agaacaccag
      301 cgcgccgatc tcgatgccgg tcggccccga ggtgctcggc cggatcctca acgtcgtcgg
      361 cgagccggtc gacgaggccg gcccggtcaa ggtggcccag cgctcgccga tccaccgccc
      421 ggcgccgaag ttcaccgacc agagcgtgaa cgtcgagatg ttcgagaccg ggatcaaggt
      481 gatcgacctg ctcgcgccgt accggcgcgg cggcaagatc ggcctgttcg gcggcgccgg
      541 ggtcggcaag accgtgctga tccaggagct gatcaacaac gtcgccaaga agtcgggcag
      601 ctactcggtg ttcgccggcg tcggcgagcg cacccgcgag ggcaacgacc tgttccacga
      661 gctcggcagc gcgatcctgt tcgacggctc gaccgtgctg tcgaagaccg cgctggtgtt
      721 cggccagatg aacgagccgc ccggcgcccg ccagcgggtc gggctgtcgg cgctgaccgt
      781 cgccgagtac ttccgcgacg tgatgaagca ggacctgctg ctgttcatcg acaacatctt
      841 ccggttcacc caggccggct ccgaggtgtc ggcgctgctc ggccgcatcc cgagcgcggt
      901 cggttaccag ccgacgctgt cgaccgagat gggcggcctg caggagcgga tcacgtcgac
      961 caaggacggc tcgatcacct cggtgcaggc gatctacgtg ccggccgacg atctgaccga
     1021 tccggcgccg gccaccgcgt tcgcgcacct cgacgcgacc acggtgctca accgcgcgat
     1081 caccgagaag ggcatctacc ccgcggtcga tccgctggac tcgacgtcga cgatcttgac
     1141 gcccgcggtg gtcggcgagc gccactacca ggtcgcgcgc gaggtccagc gcatcctgca
     1201 gcgctacaag gacctgcagg acatcatcgc gatcctcggc atggacgagc tgtccgagga
     1261 cgacaagctc accgtgtcgc gcgcccgcaa gatcgagaag ttcatgggcc agccgttcca
     1321 cgtcgccgag accttcaccg gcatgagcgg catcttctgc tcgcgcgagg acaccgtgcg
     1381 cagcttcgag gaggtcctgc agggcaagtg cgacgacctg cccgagcagg cgttcgagat
     1441 gaccggcacg atcgacgacg tgcgcgccaa ggccgccgag ctcgccaagc gcgcctagcg
     1501 ccgagggacc accatggcga gcgcaacaac cctcggagtc aacctggtca cgccgcgcgg
     1561 cgtcgtcgcc cacaccgacg ccgacagcgt ccaggcgccc ggcgagctcg gcgagttcga
     1621 gctgctgccc ggccacgtgc cgatgctggc cgcgctcaag ccgggcgtgc tcacgatcgg
     1681 caccaaggcg cgccagcggt tcgcggtgtc gtcgggctac ctgcgggtcg acctgaccgg
     1741 cgcggtcgag atcctggtcg agcaggcgat gccgaccgtc gagatcgacg ccgaggcggc
     1801 caagaaggat ctcgccgcgg ccgaggccga gctcgcgaag tggggggatc ggccgctcga
     1861 cggcgactac gtcaacatgc agcaccgggc cggctgggcg cgggcgcggc tcgacgccgt
     1921 cgcccagtga gcgcgcgcac ggggactgcc gggggctaaa attctttccc ggcggagtgt
     1981 gctcacctct gcgcacattg gcccgctgca accagcgttg acaatggcga cggtgagtcg
     2041 caaagcttga gccgtgcatc ggccccttcc gacgagctcg ggtcctgggc tcgcgatcgc
     2101 tgcggtattg gcgctcgcgg cagcccctgg ttgtggattc aatagcaaca acgggaagcc
     2161 gggcacggat gccggtcccg gtgacgatgc gccgggtggt tccggctccg atggcggacc
     2221 gatcgataca ccgccgtcgg ggaacggggt gtgctacggg ccgagcggct ggcaggtgtg
     2281 tctggatagc aaggccaccg ggtcggtgcc gctgatgggc gcgctcgaca ccgacaagag
     2341 cgatcagtgc ctgaagaacc agcccggcgg ctggaccgcg tcgcagccgg atgcctgcat
     2401 cgtcgtcggt gacaccatca ccgtgatgtc gccgagcgtc acgggaagtc ggccgctcgt
     2461 gctggttgcg cagacccaga tcaacgtcac cgggttgctc gacgttgcga gtcatcgcga
     2521 caccggcgcc gtgggggcag gagccgcgtc gtccgcggac tgcaagccgt tcggcagctc
     2581 tcccctcatg ggtcctccgg gcggcgcggg tgcaggggga ggcttcatgt tcccgggagg
     2641 ggacggcggc ccagggaacg gcgccgggat cgattcggtg gccggaggcc aggccgccgg
     2701 tgtgctggtg ggcgcgccga cccggctgcg cggcggttgc gcggggcaga ttggtggcgg
     2761 tggcaagccg agggatggcg gcgcaggcgg aggcgccgtg tatttggtct cggccggcga
     2821 gattcgaatc gagaccatgg gcaagatcga cgtgtcgggc gccgggggcg ccggtgtcga
     2881 catgctggcg ggcggcggcg gtggtggctc cggaggcatg atcgtcctct acgccccgtc
     2941 gatcaccgcg atgccaggag cgatcctgat cgccaatggc ggcggcggcg gcggcggaag
     3001 tgcgaactcg gggccgacga tgaaaggcgg cgacggtcat gacccggccg tcgtgagccc
     3061 gatcgtggcg gcgctcggtg gaaccggcgg cgtgctgatc agcgcgaacg gtggcgacgg
     3121 cggcagtggg ttccccgcga tcaccagctc caatccctcc ctcaaggggg gcaccggtga
     3181 tatcggcggt ggcggcggcg gtggtggcgg tggtggtggc tacctccggt ccaacaaggg
     3241 tctcggatcg gccgcggtgt cgccgaccgt ggacgtggtg ccctgatcga tggactccgc
     3301 ctcgcgcggc ccgggaccta tcgtgcccgg tgccggctcg aggtgggaag ctcgagaaga
     3361 tcggcgaagg tcgcacgcgt cacgtcggtg acggggtgct cgaggaacgc gagcagatcg
     3421 aggacgggct tcacgtcccc gggctggaag cgcccgtagc cgagagcatg tgctgtcccc
     3481 ttccaggtcc ggccgatgcg cgcgccgtcg gcgagctggt ggtatgcgcc gacgagccgc
     3541 gatcgatggc cagtctccgg gagccgcgcc aggcgagcat cggcccaggc cagcgcggcc
     3601 accgcgacct gcagatcagc gctcgtcggg agcttgtcga ggtcggcgag gcagcgcgtg
     3661 aacgggtcat ggttcctggt cacgaagtac agctctgcga ggtgggcgag cacgccagcg
     3721 tccctgggat ccagcgcatg cgcacgctgc agcaggcgga gcgcgatccc ggggttctgg
     3781 aggtcgtcat gggcgaccgc gcccagcctg tcgagtagca gcgcgtcggt cgggggtgca
     3841 tcggctccga ggcgcggccg tgcgtgggcc aggtcgccgg cgagcgcgtc gaggatcgca
     3901 agccggaggg cgatgattcg ctgcaattcc tggccgccac gctgctgtgc ggtcgactgt
     3961 tgtgcgatgc ctggatcgag caaggatctg gcttgctgat agcgggctcg tggtaacgac
     4021 cagacgctgg agaacatggc cacgtcgccg agggcgatca cggaccggac gcgctcgagc
     4081 tcctcgccgg gagttgccgc atgctccgag atcgggcaca ggcgatgggg cccgacggca
     4141 tctccggaag gcccacacct cgcagcttgc gcggcccagg tagatagcga tgcgccatcc
     4201 agcgcggaca gccatgggtc tgtcaggcgt tcatcgcggt cagaacggtt ggaaacatcg
     4261 gattctccgg attcgaatac gctatcctcg actccgaagg agaatcccga gacgttcgct
     4321 cgtgtggtga tgagcgagcc ggtggtggcg tcccacttgg cgccccacat cggtccattg
     4381 actctcgaag cataggcgat gacgcgcgcg ccgtcggcgc tgaacctcag cctggtgatc
     4441 gaattgttgc tgaagccgaa ttggctggac attgcgactt ccagcaacgc ctcggtcgaa
     4501 ttgcagtcct gtggcggtgg gcaggtgatg agtcgcgggg tcaccggctc tccggttgca
     4561 gcattccata cccgtacgat ggcgccgctg gctgtcacga atcgagttcc gtcggggttg
     4621 aatgcaagat gatggactcc ttgcgggtct gtgatggcga tgctcgtggg ggcgccggtc
     4681 gcggagtccc agatgcgcgc cgtgccattc ttcgcggagt cattccggat cagggtcacg
     4741 agccgggtgc catcggggct ggagatcggg ctcgagaccg ctcgcttccg ttcgtgcgcc
     4801 gcgcggtggg cggtatcggt ggtgatgtcc cagacgcgcg ccgtgccgtc ccagctggcg
     4861 gtgacgacga agtcgccgct ggggtgaaac gcggccgcgc ggacgggccc gtcgtgcggc
     4921 aatggcacgc tgaacggagc gccattcgtg gcgtaccaga cacgcgccgt gccatccagg
     4981 ctggtggtga ggacgcgtgt gcccgtgggg ctgaacgcgg cgctccagac cgcgcgttcg
     5041 tgctggagcg gggcgccgat cggagtgccg gtggcggcgt cccagatgcg cgccgtacca
     5101 tcgccactcg ccgtgacgat gcgcgtccca tcggggctga acaccgcgct gtggaccgcc
     5161 ttctcgtgcc ggaggggggc gccgatcggc atgccggtgg cggcgtccca gatccgcgcc
     5221 gtgccatctt cgctcgcggt cacgatgcgc gccccgtcgg ggctgaacac cgccgcgtgg
     5281 accgcttgtt cgtgcttgag cgggaggccg atcggcctgc ccgtgccggc attccagatc
     5341 cgcgcggtgc cgtctcggct ggcgctggcg atcagcgtgc cgtccggact gaacgacgcg
     5401 ctcagtacct cgctggcatg tgcaagtggc gcaccgatcg gagcggccga ggcgacgtcc
     5461 cacacgcgcg ccgtatgatc tccgcaggcc gtgacgatgc gcttgccatc cgggctgaac
     5521 accgcactcc agaccttcgc gtcgtgcttg aatggctcgc ggaacaggct gccggtggcc
     5581 gcattccaga cccgcgcggt gccatcctcg ctggcggtga cgacgaggct gccatccggg
     5641 ctgaacgccg cgctccacac ctccttgtca tgcgcgagcg agccgctgac cagggtgccg
     5701 gtcgcggtgt cccagatccg ggccgtacca ttcccgctgg ccgtgacgat ctgctccccg
     5761 tccgggctga agctcaccgc cgtgaggggt tggccatggc cgagcgtgat ccgccacagg
     5821 ccgctgttca cgtctgccag caggtcgcgt agctctggat cgttcatgcc caggcgcacc
     5881 gcctcggcga ggtgcgggat ggcggcagct cggtcgccac tctcgacgat caggctccgc
     5941 gcttgatctc gctcgttgag cgcgagctgg tgctcggcct cgagacgttc gcgctccgat
     6001 tcctgacgct gctgccaggc caggagtccg agcaccgtcg cggtgatcga gaacacgatc
     6061 agcgccccgg tggcaaatcg cctgagccgc ctgcgcaacc gcatgcgctc gtgctccgcg
     6121 atcgaccgtt cgcgctctcg atcttcccgc gctcgctggt ctcgccgctc ggcctcgacg
     6181 atcgccgcac ggctggccgc caggaaccgc agcaggttgt cgctgtaccc gagctcgcgg
     6241 gccgtgtcgc cggcgatcca gcgctcggcc tcgccgagct cgacggcatc gaacaggccg
     6301 acaccgccgc ggccgcgctt cgtccactcc gcggcgcgga gctcgagcag ccggcggcgt
     6361 tgctcgtcgt cgcggcgggc gagcagccag cgctggaact ccggccatcc gccgatcagc
     6421 gcctcgtgcg cgaggtcgac gagcggctcg ccgcccgcgc tcgcatccga ggtcagcatg
     6481 cgcgcgtcga tcaggcgatc gatgaccgcg tcgacgtgtg cggactcctg ccccgaggca
     6541 gcgagcgccg cacgcgattg ctggcgccgg gtgtcggggc ggccctcgcc gaagctgacc
     6601 aggcggagga agatccggcg ggcgagcgcc tgctgcggca ggctgaggcg atgcagcacg
     6661 gcgtcggcgc ggttcgccag cgcgacggcg aggccgctgc gaccggcctc accgagcgcg
     6721 tcgtagctcg ccaggcgcaa cagccggtgg ccgccgtcgc cccacagcag caccagcgcc
     6781 tcctggagca gcggcagcgc tccgggctcg gccgcggcgt cggcgatcag gcgctcgacc
     6841 agctcgcgtt cgaggtacac tccggcctcg cgtgccggcg tcacgatcgc ctcgcgcagc
     6901 gactcgccgc gcagcgcggc gacctcgagc cgggagaacc ggccctcgat gtcgggccac
     6961 agctcgctcg acatcagcgc gccatagaag tcggccctca gcgccatgac ctggtgacag
     7021 cggttgtcgg cgcggagcac gcccagcgcc tgcacgaagc gatggcgttc gtccgcgtcg
     7081 gcctgcgcga acagctcctc gagctgatcg acgaacacga gcagccgctc gcaggcctcg
     7141 cgctcgagca gcgctgccac cgcgccgccc acgctcgcgg gatcgcacgc gagcgcctct
     7201 gcgagctgcc gcatcgggga ctcgccgggg cggatcttgc gcaccgcgaa gcgatggcgg
     7261 ctcttgcgcg cgagctgcgg gatcagcccg gccgcgatca gcgacgactt ccccgatccc
     7321 gacggaccga tcacgtagat gctggggtcg cccgcgtcga gcaggccgag cagcttgctg
     7381 atctcgtcgg tgcggccgta gaactgcgac gcggtcgcgg cggtgaacgg ctgcatgccg
     7441 gggtacgggc aggcgatggg gccgaggacc gggtccggcc ggccgagccg ctcgcgaatg
     7501 cgcgcggtgg acgccgctcg ctgcgccggt gtccggcagt cgagcatggc gtgctgggcg
     7561 aggacctccg gtacgtcaca gtcggcgagc agcagcggga ccaggctacc gccgcgcccg
     7621 tcgtcgcgta cattgcgcga taacagctcc gcaaaaccca cccagcgatc ccggagatag
     7681 gccggtgaca ggatcgcgag ggtcagccgg ctgcgccgga ccgacgcggc gatcgcgtgc
     7741 tcgataaacc cggggaacgg cagctcgctg gacaggatca cgcgatcgtc gggcaacccc
     7801 agcatcggca gcagctcgcc ctggacgaac ggcgtgtcct cgtcggcgtg caggacgaag
     7861 acgtcgcgat cggtcccctc ggtcgactcg tgcggcgaca tcggcaactg gataggcgac
     7921 cggccgccag cgaatacgac aggcgtcggg cgcgttgccg cagatgtgcg tcccggtgcc
     7981 cttccgctca gtgcgccggc gtcgcgagca ccagatctgc gccgagcacc agctggctcg
     8041 gcgtgacggt cggccgcggg ccggcctgca gcgacagctg cgcgttcgcc agcgccggcg
     8101 cgaggtcgcc gaggccgagc gaggcgagat ccggcagcgg cagctggaac gtcaggttgc
     8161 tgccgatcgc gccgaacagc tgtggccaca cgaccccgga gatcaggtgg ctgacggcgt
     8221 cgggggtgag cggcccgccc ggcatcgccg aggtctccca cacgcggaga tcgggagtca
     8281 tctggatcgc gagcgacacc gtgtttccgg tgatcttgat ccgggcgccg gcggccgcct
     8341 cgatcacgaa gctctgctcg aagctgggca gctcgatctg gacctggccg agctggagct
     8401 ggacgtcgca gcgctcgccg tcgatcttgc acgccgacga cgcgggcgtc gggcggacca
     8461 gcggcggcaa cttcgcgctg acgtgggccg tgaggccggc ggacgtcagc tggccctcga
     8521 gcaggccggc gttccacagc gcgtggagca gcgcgttgag gaagtccagg tggatcccga
     8581 ggtgcagacc cgaggtgtcg agcaccgggg cgctggcgga ggtgtcgagc cgcggcgcgc
     8641 cgagcgaggc cgggtggatc ggctgcacgc ccgggatctg gaccgagagg tcctcgcgca
     8701 ccgtcacgtg accgctggtc gcaccggcgg cgatgtcgag gcccccgaag tgcccgccga
     8761 gctggagcat gaccgggctg ccgagcccgg ggtcgagcgt gaacgacagg ttggcgagca
     8821 gctggtcggc cgctccgagc agcgactgca gcagcggcgg cagctggttg atgaacgcag
     8881 ggatcgggcc ggcaagcagg ccgtcgatca gcgcgcggaa gtcgctgctg ccgagcgtga
     8941 tcagcccgtc gagctgctgg ccgttgtcgc cggtgaagtt cggggtcagc gctccgacgc
     9001 cggcggtggc gccggtgacg gtcacggcga tcgcgccgtc cgcgccggtg ccgagcgtga
     9061 gctgggccgc ggcgtggaag tctgcggtga ggccgcccgc gacgaccagc cgttggccga
     9121 cgatctggac ggtgccggtc atcgccagga acacgccgat caggtcgatg ttgagcgcga
     9181 tcgccggctg ggggccgtcg atgacacgcg cgtcgaccac gatgtccgac ggcgtgatcg
     9241 acgggatctt gatgttgatc gacgtgtcgc cctggccggc cacgatcccg tccgggggga
     9301 gcaggccggc gaggttgacg ttccacagga tcagctcgag caccgatgcc acgtcgcgcg
     9361 ccacgaccgg gttggtcgag cgatcgagcg cggtgccgag cagccgggca tcgaagaacc
     9421 gctggccgag ctggagctcc agcgcagccg gcaggtcgaa gccggtctgg cccgcgcgcg
     9481 gcggcaggta gccggcgacc cacatcacgt cgagctcctg gctgaccagc gcgacgatgc
     9541 cgtcgccgcc ctcgaccttg atgtggttga cgcccggctc ggccgcgacc tcggcggtga
     9601 acgcgccgtg cttgacgtcg acgcgcgtgc cgttgaccca cgcctcgagc gcgccccgcg
     9661 ccggggtggt ggtggcgacc gttccggtca ccgcgatcgt cgcgtggccg ccggcgacga
     9721 gctccgcgcc cggcgccggc gaggtgatcg ccacggtgat ctgggtcggg ccctcctcct
     9781 tggccatcga cgccgaggcg cagcccgcgc cgagaacgag aacgaccccg agcaagccga
     9841 gcgcgcgcat cagttcctca ccccgaacgc gccggtcagc acgtagtgcg tgccggaggt
     9901 cgacagctgg ggctggaaga tgccgagctg tgcgccggcc ggcaggccga acccggtggc
     9961 gctggggggc agcgcgaacg tcgggatcgg gaacgccggc aggccgccgt tgatcgcgtc
    10021 gaccagcacg ctctgcagga cctgggtcaa aaggcccccc atcgcggtgc gctggctctg
    10081 ggtcagcgag gcctggaacg acacgaacag ctggctcagc gtgaggttgc cgaagtgcag
    10141 cgcgtcgccc gacagggtca ccgacgcggt ggcgcgcccg ccgaactcga tcgggatcgg
    10201 cgtgtcgatg aacccgggca cccggatcgt cgcctggatc ccgccgagca tcagctgcgc
    10261 ctggttgccg gcgatcgcgg cgaccggcgg cagcaccgcg tcgatcgtcg ccgtgccgcc
    10321 ggcgagctgc agcgtggcct ggaagaaccc gccgcgccac aggccgtgca gcacctggtt
    10381 gagcacgccc tcgtacagcg acaggccgac cggggccgcg gcggtggtgc ccggcggatc
    10441 gagcagcgcg gtggtcgtgc gccccgggat gcccaggctc ggtcggttgt gcgcgaccgt
    10501 ggccgccgtg aactgggtgc cgatcccgag cagcgcgcgc gacgtcgtga tgtcgaacga
    10561 cgagaacgcg aggccgaact gcagcgggat gccgccgccg ccgtcgagcc gcggcaccat
    10621 gaacgacttg ccgaacgtgt tgatgtcgag gctcgacacc agctgatcga gcagcggcgc
    10681 gacgctcgag ttgatgaagc tggtcagcgc gttgtgcacg gcgtccttca ccgtcccggt
    10741 gaagaagctc tgcagcaggt tgaccaggaa gccgcagaac cccgagccat cgaggctgac
    10801 gttgccgacc gtcacggtcg gcgaggccgc cagcccggcg cgcagcacac cgccctgcag
    10861 ctgcaggctg aagctcacgg tcgcggcgat cgcggtcgcc cggacgctga tcgtcgagcc
    10921 gccgatgcag cacgtcgtgc cgcacgcgtt gaccgacagc cgcacgttgg gcagcgtgac
    10981 gctggcctgc agcccgccgg ggaccagggc cagcgtgctc gatggctgat cccaggccac
    11041 cgtgttcgcg ttgtaggtca cgttcggatt gcacgcgagg atgccgcagc gcccgctgct
    11101 gatcgggttg gccgcgcgca gcccgctgtc gaccaggctg cgcagctgcg tgctcgacag
    11161 cacggtgtgg aggatgtcgt tgatgctgtt gagcccggtc ggctgcggat cgccgatcgc
    11221 gttcgggtcg agccgcatcc cgatcgcccc gccgaggctc ttgtcctcgg ccgtgaagct
    11281 ctgcgcggtg agcacggtgc acgtcgtgct gttctgcttg ccgagcgtgt cggtggccac
    11341 gacgtcgacg aagttcatcc cgaagtcgac cgggacgccg gcctggtagt tgccggtggc
    11401 cgggttgacc gtcgccgccg cgccgttgat cgtcaccgac tggacgctga acgcggagct
    11461 gatcttgacc gggacgacga tcgtgctcgg cgcggcctgc agcatgtaag cgtcggacgg
    11521 ctgcgtcggt gcgtcggcgc gcatgcacgt gatcgccggg ccggacgaat tgacgagcac
    11581 cgtgcgcgtg gtggtgagcg tcttgccgtc ctgggtcggc gaggtcaccg ccgccgacag
    11641 cgtgaacgtg ccgtcctggg tgaactggaa ctgggcctcg gacggcgacg gcaggccggg
    11701 cgtcgagccg tacgacagcg cgacgtcgtc gacccggttg ccgaaccgat cgtgcgcctc
    11761 ggcgaccagc gtcacctgat cgttgaccgc gtacagcggc cgctccgggt tgaccaccgc
    11821 gaccagcgcg ctcggcaggc cggggaccac ggcgaggaag tcctccgcga cgtcggccgc
    11881 gccgctgatc acgcacgata cctggtacat cccggccgcg gtcgcggtga ccgactccgc
    11941 ggtcgtcgcg acgccgggcg ccagcggcga catcgcgaac gcctggggcg cgcccttcac
    12001 cgggttgtcg aacgcgtcga acgccaggca ggtcgcgccg accggctcgc ccgccaccgc
    12061 gaccgggttc gcgagctggg tgatcacgcg ggacggcggg ccggcgacga tctcgatgtc
    12121 gaccgcgtcg gcgaccagcc cgagcgatgg cgccgagcag cgcaccagcg ccatgccggc
    12181 gcgcgcggcg atgacctggt cctcgctgtc cctggcgaac gcgtcggtgt cctcgtaggc
    12241 gatgtcgagc tccacgctgt cggtgagcgg gttgccggcc ttgtcgagcg ccgggccgcc
    12301 ggtcgcgccg tcgatcaccg cgcagcgcgc ctcgacccgc tcgccggcgg cgatggtctg
    12361 cctggcgacc cgggtctcca cgaccagacc ggggcggtgg ttgtcgccgc agctggcggt
    12421 gaccaccagg accaggccga gcacacacgc gtggttcaac gagcgcatcg cttccccctc
    12481 gggagagtgg attttcgtag ctcagacgat accccaacta cttccttgcc ggagggccga
    12541 tgtcgagggg gggcacaacg cctggtcgac ccttgtgaac gagccccgag tcacaccccg
    12601 ccagtcgtcg cccacagctc gtgtgcgtcg gcgcacgtcg cggcccggac cagccagcga
    12661 ttcagctggc cgacgtcgtg ctcgtcgaca atccgcgcgc gctcggcgtc gcccacggcg
    12721 atgccgcgcg ccgcgagcac cgcgagcacg gcatcggcga gcgtccgcgc ggcctgttcg
    12781 acgccgcgcg ccagtccctc ctcgacgccg cgcgccagac cttcgcgcac accctcgtcg
    12841 aggcgctccg cgaggcgggc ctcgattacc ggattgtgcc gggccagcaa ggcgcgggcg
    12901 atggcatcgt ctgccttcgc ggcgtggatc atcgcctcga tcggcagcgg cacctccagc
    12961 gccggatcct cgatgtgacc ggatgcgtca agctcgcgcc agccgtcgag cgcggtcgac
    13021 cactccagca cgcgcgaccg cttgacgtcg atcatgaaca cgcggcgcac gccgcgctgg
    13081 gtcagcttgg cggccttgcg ggccgcgcgg cgccgcgacc ctttgctgat gatctcgaac
    13141 gcgagctgct ccagctggcg gcggccggtc tccgggtgcg gtgcggcggg atacacgctg
    13201 atgtcggggg caatatcgtc gaccttcgac gtccgggtca gcaggtcggc gccagcctgg
    13261 aacgccgggc cggtgtgggc ctcgatcagc gcggcgagct gcacgtggag ctccgcatga
    13321 ggtggatcgg cgggggacac gtagactagc tccccgtcga gcatctcgta cggcgtgccg
    13381 ggctcgacga gacggtcatc gatgtcgggc agctcggcct cggcgtgcgc ggcgtcggac
    13441 gccatgctcc accctacctc ggcggcagcg cgcctcggat cgggatcggt gcaatcagcg
    13501 gcgagatcat gctccgcgcc ggagagcaag cggcgcgccg aagcgaacgc agcgagatca
    13561 catccgacgc tgtcggcttt gcacgtttat tcgctctgac ctgactcctg cacatttatt
    13621 cgccgggtct gcacattaat acgacgcatc gcgttcgcgc gtcgttctcg acggagcgcg
    13681 ccgtgtctcc agctgtcaca cgggcgcagt agcgtcgtcg gcattgtcgc cgtggtggcg
    13741 acacctgaac ccacagtggc ccgccggtgg ttgcacaccg tcgagccaag gagtcgacga
    13801 tgccgaaaca tcgcaacccc cgcccgaagg tacccaagcg accacctgct ctcaaccccg
    13861 cccacaacct cacccacggc gatctgatca tccgggcgca gacggccaag ctgctctggc
    13921 gcgtggcgca cgcggcccgg gccacgctgt gggcgcccga gacggaggac ggcggcgagg
    13981 cggtgtacgc cgtcctcgac gacgcgatcg acgatctcga tgcgcacatc aggtcgggat
    14041 gcgcgtgatg atccggcggc ccgcttgcgc ggcggcccgc ggtggcaagc gctcgacctt
    14101 gcgccgcgcg cgctgatacg cttgggctgg ccgtccagtg gttcctacca cttaggtagt
    14161 gtcccgggcg atgccaacca ccggacctag tgtaactccg gagaatctcg ttgcactgcc
    14221 cggatgaaca gtgcagagtc gcggcgttca gggttgcagc cccggacacc gcggccgcga
    14281 gtcgcggcgt cactcaagca tcgtgactgc tctcgtgacg acgatgaacc tatcagatca
    14341 gatttcgtgg cgccagatca tcgacggccg cgtggtcgcc gagggcatcc gccagctg
//
LOCUS       DASVOR010001135         4651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_40916,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001135 DASVOR010000000
VERSION     DASVOR010001135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40916"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF516_21670"
     CDS             complement(<1..424)
                     /locus_tag="VF516_21670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110362.1"
                     /translation="MPEFTRRPGGGSTTPKTTGGAEPLAPGKRTLTEQLIAPAPVQRA
                     PVHPASGGASGAAPATHEPHIASPEAGIDKPGFIDNSKGAPLYSAPAEAGGELLRDAP
                     LPPAARVFASGTHHRLKHWWYVTAFVEGTMLRGYVEDFR"
     gene            complement(869..1060)
                     /locus_tag="VF516_21675"
     CDS             complement(869..1060)
                     /locus_tag="VF516_21675"
                     /inference="COORDINATES: protein motif:HMM:NF038153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HEX8110363.1"
                     /translation="MRKSERRLTKKLMLNSETVRTLTRRELTEVAGGVEDSHETCVAK
                     VLLVDSHDPCTAKMPLAPP"
     gene            complement(1103..1267)
                     /locus_tag="VF516_21680"
     CDS             complement(1103..1267)
                     /locus_tag="VF516_21680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HEX8110364.1"
                     /translation="MRKKPPKLALRKETLRALSDLQLAHVAAGQETDAPLIDVTRGNV
                     CPAQDAAKNA"
     gene            complement(1267..4650)
                     /locus_tag="VF516_21685"
     CDS             complement(1267..4650)
                     /locus_tag="VF516_21685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110365.1"
                     /translation="MGEGGGGTVYRCFQPSLQRHAVVKVLREPKRGDNGAKERFLREA
                     QLASQLDHPCAAHVYAFGAEPDGVLWIAMELVKGTTLEAWLRKRGAMPPEEFVPVFEC
                     VAEAVNCAHERGIVHRDLKPSNVMLTEHGGRRIAKLLDFGIAKADNESIALSLDPLVG
                     ATRRADADGPVTNRDPAERDWHLTPSDVGIGSAPYMSPEQWDNARAVGPASDIYSLGV
                     LAYRALTGRVPFPAESEAKCREQHLHMEPPPLGGDLPPSFDRAIRRALAKAPEARHGS
                     ALDLASELRGALPASVRELLRSSAGRWAARARRPGLLWSSDALADVDRWTREAPSGAL
                     SELECSFVAASRTRARHIRWTTRSFAALVAAAAVVTLMWRSAMHARLAETEARDARAL
                     AEAKVAESELERGRAALLHGEPEALAHLAEAYRRDPAPTTAFMFARAAQSRLAEKARL
                     ASTQGRMWWATFSPDGGRIATADDRAAQLWDATSYRLLFTLPHGCEVYQAIFSADGTR
                     LVTIARTMVRIWDARSGALLLDLKAGRGQTPSDFYRGTVSSDGRFVAAMDAGGTLASV
                     WDVELGTLVARLRNQGGDFPNLAFGADGWLATTAGEDARVFEPRGWTQVGVIHGPVRS
                     LVLGARGRMMTGSSTGEVTLWSIPGATMLRQLRKFGEPVEAVAFSPDGTLVAAGGGDG
                     AVQVWQADSGMLRSQFNPRHGKIVDVEFDPKAVRLLAATSDGTAVIADVSQGLPLAVL
                     DGPSNALRVARFAPDGLRVIGASWDGAATVWEGASPYRRWAAAAEGDACDIGMSSRAD
                     RRFIAVGCRGLPTRVWDTSRDLLLAELPSTTPSEANDFMSAAPAVSPAGDLAAVPRGA
                     TVELYDLPGGRPRRAVEHEAPVSAIAFTDADFGHAIASGALDGSVRVVREDGTDLALQ
                     ASGGVEAVAVLPDGRVIVADAERRLRVYAADGTVIASLELPARVMSLRREGARLVALP
                     NCVATTAPPFLIDLERYRVDAKLEGHVGCVLSARWSSGQRVLTAGADGTARLWDGSTG
                     HLLQTFKGSSRFLGDAVLMSGMAIAGDANGVLRFWDAASGAKLWTLPAHKSAVIGVHL
                     EGADIVTRGSTGEISRWRLPQSGAVIDACARARPCGIVP"
BASE COUNT          691 a         1657 c         1618 g          685 t
ORIGIN      
        1 cgcggaagtc ctcgacgtag ccgcgcagca tcgtcccctc gacgaacgcg gtcacatacc
       61 accagtgctt cagcctgtga tgcgtcccgc tcgcgaagac gcgggcggct ggagggagcg
      121 gcgcgtctcg cagcagctcg ccgccggcct cggccggcgc gctgtagagc ggagcgcctt
      181 tggagttgtc gatgaacccg ggcttgtcga tgccggcttc cggcgacgcg atgtggggct
      241 cgtgagtggc gggggcggca ccggacgctc cgccagacgc cgggtgaacc ggagcgcgct
      301 gcacgggtgc aggcgcgatc aactgctcgg tgagcgtgcg cttgccgggc gcgagcggct
      361 ccgccccgcc ggtcgttttc ggcgtcgtac tgcccccacc cggcctacgc gtgaactcag
      421 gcacctgttt ctcattctcc ggccctacag cgttgtcatt cccgttccga cgccacaccg
      481 aacgcgcggg cccggaacca gtccacagcg ccgcggaccc taacacggca ggcgagaagc
      541 tctcgctcag ctgatgcctt cttcgtcgtg agcgcgaacg aactcaccgt cgtcgcgtca
      601 tcagcctctg tcacgagcgc gagcgcggct acaagcccgt agctttcgcg gcgcgagtcg
      661 ctcggggcct acaatctgca agcggatctc cggcgacact tgcggacgat gccgagctgc
      721 gcgagtctgc cgacacgggg cagtcgctgg caacgctcaa ccgatccgat acgacatggc
      781 agccgtcggc aatgcacgga cacgcgcgcg gtgcgacgtc agcgcgggcc gcaatcattc
      841 agttgtcttc aaccgcaggg agcggcgctc acgggggcgc gagcggcatc ttggcggtgc
      901 atggatcgtg cgagtccacg agcagcacct tggcgacgca ggtttcatgc gagtcctcga
      961 caccacctgc gacctccgtc aattctctcc gcgtgagcgt ccgcacggtc tcgctgttca
     1021 gcatgagctt cttggtcagc cggcgctcgg atttcctcat cagggtacga tagctcggcg
     1081 ctgccagtag ctacggagcc catcacgcgt tcttcgcagc gtcctgcgcg ggacatacgt
     1141 tgccgcgggt gacgtcgata aggggggcat cggtctcttg gcctgcggcg acatgggcga
     1201 gttgcaggtc ggagagcgcc cgaagggttt ccttgcgcaa cgcgagcttg ggtggttttt
     1261 ttcgcatcac ggcacgatac cgcaggggcg agcgcgggcg caggcgtcga tcactgcccc
     1321 ggattgcggc aaccgccagc gcgagatttc tccagtggat ccgcgcgtca cgatgtcggc
     1381 cccttcgaga tggacgccga tcaccgcgga cttgtgcgcc gggagcgtcc agagtttggc
     1441 gccgctcgcc gcgtcccaga accggagcac gccgttggcg tcgccggcga tcgccatgcc
     1501 ggacatgagc accgcgtcgc ccaggaacct cgagctgccc ttgaaggtct ggaggagatg
     1561 tcctgtggag ccgtcccaca gcctggccgt cccgtcggcg ccggcagtca ggacgcgttg
     1621 ccccgatgac catcgcgccg agagcacgca gccgacatgg ccttcgagct tcgcgtcgac
     1681 ccgataccgc tcgaggtcga tcaggaaagg cggcgcggtg gtggcgacgc agttcgggag
     1741 cgcgacgagg cgcgcgcctt cccgccgcag cgacatgacg cgcgccggaa gctcgagcga
     1801 ggcgatgacc gtgccgtccg cggcgtacac gcggaggcga cgttcggcgt cggcgacgat
     1861 gacacgcccg tccggcagga ccgccacggc ctcgacgccg cccgatgcct ggagcgcgag
     1921 atcggtcccg tcttcgcgga cgacccgcac cgagccgtcg agcgcgccgc tcgcgatggc
     1981 gtggccgaag tccgcgtccg tgaatgcgat ggcgctgacc ggcgcctcgt gctcgacggc
     2041 gcggcgcggc cgacctcccg gaagatcgta gagctcgacg gtcgcgccgc gcgggaccgc
     2101 ggcgagatct cccgccggag agaccgccgg ggccgcggac atgaagtcgt tggcctcgga
     2161 cggagtcgtg ctcggcagtt cggcgagcaa caggtcgcgc gaggtgtccc agacccgggt
     2221 cgggaggcct cggcagccga ccgcgatgaa ccgccggtcc gcccgcgagc tcatgccgat
     2281 gtcgcaggcg tcaccctccg ccgccgcggc ccagcgccga tagggggagg ccccctccca
     2341 caccgtggcc gctccgtccc aggacgcgcc gatcacgcga agcccgtccg gggcgaagcg
     2401 cgcgacgcgc agcgcgttcg atgggccgtc caggaccgcg agcggcagcc cctgcgagac
     2461 gtcggcaatc acggcggtgc catcggaggt cgctgccagg agccgcacgg ctttcgggtc
     2521 gaactcgacg tcgacgatct tgccgtgcct tgggttgaac tggctccgca gcatgccgga
     2581 gtccgcctgc catacctgga ctgcgccgtc gccgccaccg gcggcgacga gcgtgccgtc
     2641 gggcgagaac gccacggctt cgaccggctc gccaaacttg cgcagctgcc ggagcatcgt
     2701 cgcgcccggg atcgaccaga gcgtgacttc gccggtcgaa gatccggtca tcatgcggcc
     2761 ccgcgcgccg agcaccaggc tgcggacggg gccgtggatg acgccgacct gcgtccatcc
     2821 ccgcggttca aagacgcgcg catcttcgcc cgccgtcgtc gccagccagc cgtccgcgcc
     2881 gaacgcgaga tttgggaagt cgccgccctg gttgcgcagc cgggcgacga gcgtgccgag
     2941 ttcgacgtcc catacgctcg ccaaggtacc gccggcgtcc atcgcggcga cgaatctccc
     3001 gtcggaggag acggttccgc ggtagaaatc agagggcgtc tgcccgcgtc ccgccttgag
     3061 gtcgaggaga agcgcgccgc ttcgcgcgtc ccagatcctg accatcgtcc tggcgatggt
     3121 cacgagccgc gtgccgtcgg cagagaagat ggcctgatag acctcgcagc cgtgcgggag
     3181 cgtgaagagg agccggtacg acgtcgcatc ccagagctgg gccgctcggt cgtcggccgt
     3241 ggcgatccgc ccgccgtccg gagagaaggt tgcccaccac atgcggccct gcgtgctcgc
     3301 gaggcgcgcc ttctccgcca gccgggactg cgccgcacgg gcgaacatga acgccgtcgt
     3361 cggcgccgga tcccgccggt acgcctccgc gaggtgagcg agcgcctcgg gctccccgtg
     3421 cagcagcgcc gcccggcccc gctcgagctc ggattcggcg accttcgcct cggcaagtgc
     3481 gcgggcgtcc cgcgcttccg tctcggcgag acgtgcatgc atcgccgaac gccacatcaa
     3541 cgtgacgacg gcggccgccg cgacgagcgc cgcgaacgag cgcgtggtcc agcggatgtg
     3601 cctcgcgcgc gtccggctcg ccgccacgaa cgaacattcc agctccgaga gcgcccccga
     3661 cggagcctcg cgcgtccagc ggtcgacatc ggcgagcgcg tcgctactcc agagcagtcc
     3721 aggccttcgg gctcgggcgg cccatcgccc tgccgacgag cggagcagct cgcgcacact
     3781 cgccggcagc gcgccgcgca gctccgacgc caaatcgagc gcgctgccgt gccgcgcctc
     3841 aggcgccttg gccaaggcgc gccggatcgc gcgatcgaag ctcggcggga ggtcgccgcc
     3901 gagcggcggc ggctccatat ggagatgctg ttcgcggcac ttggcctcgc tctcggcggg
     3961 gaacggcacg cgtccggtga gcgcccggta ggccaggacg ccgagcgaat agatgtcgga
     4021 cgccggaccc acggcccgcg cgttgtccca ctgttcgggc gacatgtatg gcgcggagcc
     4081 gatcccgaca tcggacggcg tgaggtgcca gtcgcgttcc gcggggtccc tgttggtcac
     4141 cgggccgtcg gcgtccgcgc gccgggttgc cccgaccagg ggatcgaggg agagggcgat
     4201 gctctcgttg tccgccttgg cgatgccgaa gtcgagcagc ttggcgatcc gacgtcctcc
     4261 gtgctcggtc aacatcacgt tcgagggctt gaggtcgcga tggacgatcc cacgttcgtg
     4321 cgcgcagttg accgcctcgg cgacgcactc gaagaccggc acgaactcct cgggtggcat
     4381 cgctccgcgc ttccggagcc acgcttcgag cgtcgtgccc ttcaccagct ccatggcgat
     4441 ccacagcacg ccgtccggct cagcgccgaa ggcgtacacg tgggcggcgc acggatgatc
     4501 gagttgcgac gcgagctgcg cctcgcgaag gaagcgttcc ttcgctccgt tatcgccgcg
     4561 cttcggctcg cgcagcacct tgaccaccgc gtgacgctgg agcgacggct ggaagcagcg
     4621 atacacggtg ccgccgccgc cctcgccaat c
//
LOCUS       DASVOR010001136         3413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_508173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001136 DASVOR010000000
VERSION     DASVOR010001136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3413
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..187)
                     /locus_tag="VF516_21690"
     CDS             complement(<1..187)
                     /locus_tag="VF516_21690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110366.1"
                     /translation="MRLSRYVGFIDLGVATVVLVALVLPAREMYASAVGAGSDAEQFA
                     LALAEARTMARPEDGAAI"
     gene            433..1074
                     /locus_tag="VF516_21695"
     CDS             433..1074
                     /locus_tag="VF516_21695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8110367.1"
                     /translation="MLTSISTHFHQGGPGMWPILFTSVVTIAIIVERAIYLFRSSVDK
                     DKLLALLKSQVMSGNVQGAIKVCSGNPTPMTRIVQAGLMKFNKSDDEVQAAMDEAALR
                     ELPKINARTPYLAMTANFAVMAGLLGTIGGMIGSFGSVASADSGNRSTELANGVAEAL
                     NCTFFGIFTSLVALLGFSLLQGKTTHVIDDINEVTVQVVNLVTSHRAQMQPSA"
     gene            1163..1669
                     /locus_tag="VF516_21700"
     CDS             1163..1669
                     /locus_tag="VF516_21700"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8110368.1"
                     /translation="MAASLGTGDKKSVNIELNIVPFIDLMSCLTSFLLVTAVWINIAQ
                     INIQPKGKSRDQAQVQQDDERPILSVLLQSDQIWLGLSRPSDEFRKIPKKGDQQDWEV
                     FETTLKEYKSSAFFNDRSDLEIAAESTAAAPVKYQDIIHAMDIAVRVGFLDVGLSDPQ
                     GLSARPQQ"
     gene            1681..2289
                     /locus_tag="VF516_21705"
     CDS             1681..2289
                     /locus_tag="VF516_21705"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8110369.1"
                     /translation="MAIQSAGPRLYRSVKFKHLVKGSGSAAGGRASNISLNLTPFVDM
                     MTILVTFLLMVFSASGELLQAVKGLELPSAQAHDTLQQAPVIIVTKTEITYQGQLVAT
                     VDSVMRDDSPTFKIDALFERLDAAAKKIREDVAYGRQAKDLLKSCEDAKQNIRPAAGK
                     ICPDGLAILQADESTDGQLINKIVNTAKAAGFDNLLFAVKNK"
     gene            2309..>3413
                     /locus_tag="VF516_21710"
     CDS             2309..>3413
                     /locus_tag="VF516_21710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110370.1"
                     /translation="MMRRFSWFPIVAGLAVLAGCPDDPYKAETWTKKLKDQRESERAV
                     TELEQLGNPSAIPALGDAWVDQGKPVRLLQVIISLARPLTPEQAKANYFTDYEATGRP
                     PNWERALPYLKRALSEVDEANPRSVDSATKAAEALGEAKLPDGLDALIEIAQKPVTKK
                     LITAQVAALRAIGKYTGDSQRASAALIKLIDKDPPANPRTARDRDQGRALEEKYALFL
                     GFTGAAINALGNLHVAAAARTLVLAMFRTPELFTLTRRALVASGPAAFDELRKVLAGT
                     HPEVNQLFKDKRLDKYCGDRNDAPPDQCQPVSAMVFFPAVVLGDFYDARAAPELLAAL
                     QRPALPVYYIDDQPSPNTQYNAIFDALRKIGAPE"
BASE COUNT          624 a         1201 c         1086 g          502 t
ORIGIN      
        1 cgatcgccgc gccgtcctcc gggcgcgcca tggtccgcgc ctcggcgagc gcgagcgcga
       61 actgctcggc gtcgctcccg gcgccgaccg cgctcgcata catctcccgc gccggcagca
      121 ccagggccac caggacgacc gtggcgaccc cgagatcgat gaagccgaca taccgcgaaa
      181 gccgcatgtc tcctttgaca tgacccatcg ccacaggttc ccggctttcg ccgcttggtt
      241 ccccacgtag atgctggtgg cgccgtagca tttcgcctcg acgcgtcccg agatgctacg
      301 gcccgaggcg atgtacttat ttgtacaaca caaggcatct cgccaggttg cgccgtggat
      361 ccgggcttgc actgccgcag cgcccagacc tgcactcgca tccccgacct cggaccatcg
      421 gagaggaagt tcatgttgac ctcaatctcg acgcatttcc accagggcgg cccgggcatg
      481 tggcccattc tgttcacctc ggtggtgacc atcgcgatca tcgtggagcg cgcgatctat
      541 ctgttccgct cgtcggtcga caaggacaag ctgctcgccc tgctcaagag ccaggtgatg
      601 agcggcaacg tgcaaggcgc gatcaaggtg tgctcgggca acccgacgcc gatgacccgg
      661 atcgtgcagg ccggcctgat gaagttcaac aagagcgatg acgaggtcca ggccgcgatg
      721 gacgaggccg cgctgcgcga gctccccaag atcaacgcgc gcaccccgta cctggcgatg
      781 acggcgaact tcgcggtcat ggcgggtctg ctcgggacga tcggcggcat gatcggctcg
      841 ttcggctcgg tcgcctccgc cgacagcggc aaccgctcga ccgagctcgc caacggcgtc
      901 gccgaggcgc tcaactgcac gttcttcggc atcttcacgt cgctggtcgc gctgctcggg
      961 ttctcgctgc tgcagggcaa gaccacgcac gtgatcgacg acatcaacga ggtcaccgtc
     1021 caggtggtca acctggtgac gtcgcaccgc gcccagatgc agccctcggc gtagccctcg
     1081 cacgatgacc gaccacacac cacacaccgg cacatcgccg ggcgtggcgt gcccccagaa
     1141 tcgctagcga ggagttctca ccatggctgc atccctcgga accggcgaca agaagagcgt
     1201 caacatcgag ctcaacatcg tgccgttcat cgatctgatg agctgcttga cctccttctt
     1261 gctggtcacc gcggtgtgga tcaacatcgc ccagatcaac atccagccca agggcaagtc
     1321 gcgcgatcag gcgcaggtcc agcaggacga cgagcgcccg atcctctccg tgctgctcca
     1381 gtccgatcag atctggctcg gcctgtcgcg gcccagcgac gagttccgga agatccccaa
     1441 gaagggtgat cagcaggact gggaggtgtt cgagaccacg ctcaaggagt acaagagctc
     1501 ggcgttcttc aacgaccgct cggacctcga gatcgccgcc gagagcacgg cggcggcgcc
     1561 ggtgaagtac caggacatca tccacgcgat ggacatcgcc gtccgggtcg ggttcctgga
     1621 cgtcggactc tccgacccgc agggcctctc ggcccggcca cagcagtaaa ggaggcgatc
     1681 gtggcaatcc agtcagcagg ccctcgcctc taccgctcgg tcaagttcaa gcacctggtg
     1741 aaaggtagcg ggagcgcggc cggcggcagg gcatccaaca tctccctcaa cctgacgccg
     1801 ttcgtcgaca tgatgacgat cctcgtcacc ttcctgctca tggtgttctc ggccagcggc
     1861 gagctgctgc aggccgtgaa gggcctcgag ctgccgagcg cgcaggccca cgacaccctg
     1921 cagcaggccc cggtcatcat cgtcaccaag accgagatca cctaccaggg gcagctggtg
     1981 gcgacggtcg acagcgtgat gcgcgacgac tcgccgacct tcaagatcga cgcgctgttc
     2041 gagcggctcg acgcggcggc caagaagatc cgcgaggacg tcgcctatgg ccggcaggcc
     2101 aaggacctgc tcaagtcgtg cgaggacgcc aagcagaaca tccgtccggc ggccggcaag
     2161 atctgccccg acgggctggc gatcctgcag gccgacgagt cgaccgacgg acagctgatc
     2221 aacaagatcg tcaacacggc caaggcggcg ggcttcgaca acctcttgtt cgcggtgaag
     2281 aacaagtgaa gagagcgagc ggactaccat gatgcgacgg ttttcgtggt tcccgatcgt
     2341 tgcgggactg gcggtgctcg cgggttgtcc ggacgatcct tacaaggccg agacctggac
     2401 caagaagctc aaggaccagc gcgaatccga gcgcgcggtg accgagctcg agcagctcgg
     2461 caacccgagc gcgatcccgg cgctcggcga cgcgtgggtc gaccagggca agccggtgcg
     2521 cctgctccag gtgatcatct cgctggcccg gccgctcacc cccgagcagg ccaaggccaa
     2581 ctacttcacc gattacgagg ccaccggccg gccgccgaac tgggaacgcg cgctgcccta
     2641 cctcaagcgg gcgctgtccg aggtcgacga ggccaacccg cgctcggtcg acagcgcgac
     2701 caaggcggcc gaggcgctcg gcgaggccaa gctgcccgac gggctcgacg cgctgatcga
     2761 gatcgcccag aagccggtga ccaagaagct gatcaccgcg caggtcgccg cgctccgcgc
     2821 gatcggcaag tacaccggcg acagccagcg ggcgtccgcc gcgctgatca agctgatcga
     2881 caaggacccg ccggccaacc cgcggaccgc gcgcgaccgg gaccaggggc gcgcgctcga
     2941 ggagaagtac gccctgttcc tcggcttcac cggcgcggcg atcaacgcgc tcggcaacct
     3001 ccacgtcgcc gccgcggcca ggaccctggt gctcgcgatg ttccgcacgc cggagctgtt
     3061 cacgctgacc cggcgcgcgc tggtcgcgag cgggccggcg gcgttcgacg agctgcgcaa
     3121 ggtgctcgcc gggacgcacc ccgaggtcaa ccagctgttc aaggacaagc ggctcgacaa
     3181 gtactgcggc gaccgcaacg acgcgccgcc cgaccagtgc cagccggtgt cggcgatggt
     3241 gttcttcccg gcggtcgtgc tcggcgactt ctacgacgcg cgcgcggcgc ccgagctgct
     3301 cgccgccctg cagcgcccgg cgctcccggt gtactacatc gacgaccagc cgagccccaa
     3361 cacccagtac aacgcgatct tcgacgcgct ccgcaagatc ggcgcgcccg agg
//
LOCUS       DASVOR010001137         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_216181,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001137 DASVOR010000000
VERSION     DASVOR010001137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216181"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1122
                     /locus_tag="VF516_21715"
     CDS             <1..1122
                     /locus_tag="VF516_21715"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110371.1"
                     /translation="LVLSVFVCAAIVAIAWTQGPGCVRMSVAVLSVVAVGSVLNTRLT
                     AVVGLGLAAIIGGLGASEAAGLYHGLDLGPDLGLGDAARDAAYGPFIRQSISLGVMVI
                     VLRRSYDRLALHVRDRERACRDAVDRVRAINSSLEARVAERTAVLQHTRDQLRELAAQ
                     LTTDVRGHLGAIRRQLDAFATTEAALGPAALRDVAKASSAAARLSVMTERLHEHASVG
                     TTALRPAWIAMDALIREVIDEYDRAAGPGVEWQVEALPPAWGDPALVRTVVENLIGNA
                     VKFSRNRTPPRIHIGHDPARGYFVRDNGVGFDLGRAGKLFSPFHRLHREDEFEGHGLG
                     LANVRRILHHSGGEITAEGELDRGATFFFWLAPPSSESP"
     gene            1119..1991
                     /locus_tag="VF516_21720"
     CDS             1119..1991
                     /locus_tag="VF516_21720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110372.1"
                     /translation="MTERLFRCFLVHPIDDPDQARRARLIATATAVTALYLVGVTALY
                     AVGGHRTAMLIALSVAALAALTHALARTGRLPALELATAALAAYGAVALVALQGLHSA
                     RLGALHIVVAFLGLAARPWMAAAQVAFQIAILATATVAGLAIPLSPPTTPVWVDVVLQ
                     LLLITVLMMIFTHGYQRLLAALTQSTAKLEASHAELVAASARLERLVSERAIELERAS
                     RDLEAFATTISHDLQAPLRHVRQYLALFIDDAAALGDDRLAPIIAAQHSAVELTAKIE
                     AILSAHRRASSGRS"
     gene            complement(2028..>2416)
                     /locus_tag="VF516_21725"
     CDS             complement(2028..>2416)
                     /locus_tag="VF516_21725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110373.1"
                     /translation="PPPARPEPVAPTPAAPPPETRPRPAAPPPAPAKTTPLVAPTAVS
                     KLSGEIPAMKVTGVTEASTDVVTKMCIDEHGRVTSVKIVKALPEIADELRRSLSGWRY
                     KPYTNAAGQSSPACFPLTLKVVFKRE"
BASE COUNT          303 a          918 c          838 g          357 t
ORIGIN      
        1 ctggtgctga gcgtgttcgt ctgcgcggcg atcgtggcga tcgcatggac ccaggggccc
       61 ggctgcgtcc ggatgagcgt ggcggtgctc agcgtggtgg cggtgggctc ggtgctcaac
      121 acgcgattga cggccgtggt cggcctcggc ctcgccgcga tcatcggcgg gctcggggcg
      181 agcgaggcag ccggcctcta ccacggcctc gatctcggcc ccgatctcgg cctcggggat
      241 gccgcgcgcg acgctgccta cgggccgttc atccggcagt cgatctcgct cggggtcatg
      301 gtgatcgtgc tgcgccggag ttacgaccga ctcgccctcc acgtccgcga ccgcgagcgc
      361 gcctgccgcg acgccgtcga ccgcgtccgg gcgatcaact catcgctcga ggcgcgcgtc
      421 gcggagcgca cggccgtgct ccagcatacc cgcgaccagc tgcgcgagct cgccgcccag
      481 ctcaccaccg atgtgcgcgg ccacctcggc gcgatccgcc gccagctcga cgcgttcgcg
      541 accaccgagg ccgcgctcgg gcccgccgcg ctgcgcgacg tcgccaaggc gagcagcgcc
      601 gcggcccggc tctcggtgat gaccgagcgg ctgcacgagc acgcgagcgt gggcaccacc
      661 gcgctgcgtc cggcgtggat cgcgatggac gcgctgatcc gcgaggtgat cgacgagtac
      721 gaccgcgccg cgggtcccgg cgtcgagtgg caggtcgagg ccttgccacc tgcgtggggc
      781 gaccccgcgc tggtccgcac cgtggtcgag aacctgatcg gcaacgcggt caagttcagc
      841 cggaaccgca ccccgccgcg catccacatc ggccacgacc ctgcgcgcgg ctacttcgtg
      901 cgcgacaacg gcgtcggctt cgacctgggc cgggccggca agctgttctc gccattccac
      961 cgcttgcatc gcgaggacga gttcgagggc catggcctcg ggctggccaa cgtccggcgc
     1021 atcctccacc acagcggcgg cgagatcacc gccgagggcg agctcgatcg tggcgcgacc
     1081 ttcttcttct ggctcgcgcc tccgagcagc gagagcccat gacggagcgc ctgttccgct
     1141 gcttcctggt tcacccgatc gacgaccccg accaggcgcg gcgcgcccgg ctgatcgcga
     1201 ccgcgaccgc ggtgaccgcg ctctacctgg tcggcgtcac cgcgctctac gcggtcggtg
     1261 gacaccgcac cgccatgctg atcgcgctga gcgtcgccgc gctcgccgcg ctgacccatg
     1321 cgctcgcccg gaccggccgc ctgccggccc tcgagctcgc gaccgcagcg ctggccgcgt
     1381 acggcgcggt tgcgctggtc gccctgcaag gcctgcactc cgcccggctc ggcgcgcttc
     1441 acatcgtcgt ggcgtttctc ggtctggcag cgcggccctg gatggcggcg gcgcaggtgg
     1501 cattccagat cgcgatcctc gcgaccgcca ccgtggccgg tctggcgatc ccgctgtcgc
     1561 cgcccaccac cccggtctgg gtcgacgtcg tcctccagct gctgctgatc accgtcctga
     1621 tgatgatctt cacgcacggc tatcagcgcc tgctcgccgc gctgacccag agcacggcaa
     1681 agctcgaggc ctcccacgcc gagctggtcg ccgccagcgc ccggctcgag cgcctggtca
     1741 gcgagcgcgc gatcgagctc gaacgcgcca gccgcgatct cgaggcattc gccaccacga
     1801 tctcgcacga cctccaggcg ccgctgcgcc acgtccgcca gtacctcgcg ctgttcatcg
     1861 acgacgccgc tgcgctcggc gacgaccgtc tcgcgccgat catcgcggcg cagcattcgg
     1921 cggtcgagct gacggccaag atcgaggcga tcctgtcggc ccatcgccgc gcgtcctccg
     1981 ggagaagctg acaccgatcg gccgaatgcc gaggccgagc accggcgcta ctcccgcttg
     2041 aacacgacct tgagcgtcag cgggaagcac gccggcgagg actggccggc cgcgttggtg
     2101 tacggcttgt agcgccagcc ggacaggctg cgccgcagct cgtcggcgat ctcgggcagc
     2161 gccttgacga tcttcaccga cgtcacgcgg ccgtgctcgt cgatgcacat cttggtgacg
     2221 acgtccgtgg acgcctcggt cacgccggtg accttcatcg ccgggatctc gcccgacagc
     2281 ttggagaccg cggtcggcgc caccagcggg gtcgtcttcg ccggcgcggg cggtggcgct
     2341 gccggcctgg ggcgggtctc cggcggcgga gcagccgggg tcggtgccac gggctcgggc
     2401 ctggccggcg gcggct
//
LOCUS       DASVOR010001138         2574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_69791,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001138 DASVOR010000000
VERSION     DASVOR010001138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69791"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2069)
                     /locus_tag="VF516_21730"
     CDS             complement(<1..2069)
                     /locus_tag="VF516_21730"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8110374.1"
                     /translation="MQEVASSIDRYQVVRRIGAGGMGVVYEADDVERGQKVALKTISN
                     LDVEKIYQLKREFRALADLSHPNLVALYDLVVAADSCFFTMELLDGVDLLTYLWGRKV
                     EDPAELMPTTLTPIPPGFVGADDDSEDNTMHSPRPTPCDIDRLRHALPQLTRGLHALH
                     SAGKIHRDVKPSNIQVTSDGRVVLLDFGLVAELERRQGADGMIVGTVAYMAPEQCAGD
                     VQLSPAADWYGLGVVLFQALTGRLPFEGVPTRVLFEKQTEAAPRPSMLVQHIPRDLDD
                     LCAELLEREPADRPTGAQLLHRLGVGDTDRMATALASSSREAGFAGRSHELAMLEAAL
                     EPLAIKRASVALVRAPSGMGKTSLVNRFLERLRATRPNALVLHGRCLDREDVPYKAMD
                     PLIDELSDWWLGLTPKQAQALLPRDAYLLPTLFPVLDRVPAIADAPRTRIVADPQARR
                     THAFGALRETLQRLGDRHTVVLFLDDMQWVDRDTTALLADLMRAPDPPPVLLVLATRT
                     EGAEPVVDLVRRMDAAQRLIDLGPLDDAAAIAIAVSHLGEDNRDTAVQLVAEADGSPL
                     FLIELARYLHGRRIEDIAGMGLDAMLAERIGGLGDAARMIAELVAVAGEPMTRSALAS
                     ISAVAPAELSRQLSLLRAQRVVRMSGSRAEDTIEPYHARVRGAVLSQLDDDRRARHHR
                     ALAIALSG"
     gene            complement(2093..>2574)
                     /locus_tag="VF516_21735"
     CDS             complement(2093..>2574)
                     /locus_tag="VF516_21735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110375.1"
                     /translation="VRLQPVAKDDAAYGDQDLLLGTFESFATAARDRARTDAGDYLAN
                     HYSTVTPWWLTGHGPVILRLVPPPPAAADRGSDRLARLDADIAAGRAGFTLMTDTGSG
                     ALALADLQLTERIDIDDRRLRASMFRQGRGVRPLGLRNGIRATVYPMSQVARRLRGG"
BASE COUNT          339 a          911 c          980 g          344 t
ORIGIN      
        1 cccgacagcg cgatcgccag cgcgcggtgg tggcgggcgc ggcgatcgtc gtcgagctgg
       61 ctcagcaccg cgccgcggac ccgggcgtgg tagggctcga tggtgtcctc ggcgcgcgac
      121 cccgacatcc ggaccacgcg ctgggcgcgg agcagcgaca gctggcgcga cagctcggcg
      181 ggcgcgaccg cgctgatcga cgccagcgcc gaacgggtca tcggctcgcc ggcgaccgcg
      241 accagctcgg cgatcatccg cgcggcgtcg cccaggccgc cgatccgctc ggcgagcatc
      301 gcgtcgagcc ccatgccggc gatgtcctcg atccggcggc cgtgcaggta gcgcgcgagc
      361 tcgatcagga acagcgggct gccgtcggcc tcggcgacca gctgcaccgc ggtgtcgcgg
      421 ttgtcctcgc cgaggtgcga caccgcgatc gcgatcgccg cggcgtcgtc gagcggcccg
      481 aggtcgatca gccgctgcgc cgcgtccatc cggcgcacca ggtcgaccac cggctcggcg
      541 ccctcggtcc gggtcgcgag cacgagcagc accggcggcg ggtcgggcgc gcgcatcagg
      601 tcggcgagca gcgcggtggt gtcgcgatcg acccactgca tgtcgtcgag gaacagcacc
      661 acggtgtggc gatcgccgag ccgctgcagg gtctcgcgca gcgcgccaaa cgcgtgggtc
      721 cggcgcgcct gggggtcggc gacgatccgg gtgcgcggcg cgtcggcgat cgccggtacg
      781 cggtcgagca ccgggaacag ggtcggcaag aggtacgcgt cgcgcggcaa cagcgcctgg
      841 gcctgcttgg gggtcagccc gagccaccag tcgctgagct cgtcgatcag cgggtccatc
      901 gccttgtacg gcacgtcctc gcgatcgagg cagcggccgt gcagcacgag cgcgttgggc
      961 cgggtcgcgc gcagccgctc gaggaagcgg ttgaccagcg aggtcttgcc catccccgac
     1021 ggcgcgcgga ccagcgccac cgaggcgcgc ttgatcgcga gcggctcgag cgcggcctcg
     1081 agcatcgcga gctcgtggct gcggccggcg aacccggcct cgcgcgagct gctggccagc
     1141 gcggtcgcca tccggtcggt gtcgccgacc ccgagccggt gcaggagctg ggccccggtc
     1201 ggccgatcgg cgggctcgcg ctcgagcagc tcggcgcaca ggtcgtcgag gtcgcgcggg
     1261 atgtgctgga ccagcatcga cggccgcggc gcggcctcgg tctgcttctc gaacagcacg
     1321 cgggtcggca cgccctcgaa cggcagccgg ccggtcagcg cctggaacag cacgacgccc
     1381 aggccgtacc agtcggcggc cgggctgagc tggacgtcgc cggcgcactg ctcgggcgcc
     1441 atgtacgcga cggtcccgac gatcatgccg tcggcgccct gccgccgctc gagctcggcg
     1501 accaggccga agtcgagcag cacgacgcgg ccgtcgctgg tgacctggat gttcgacggc
     1561 ttgacgtcgc ggtggatctt gcccgcgctg tgcagcgcgt ggagcccgcg cgtcagctgg
     1621 ggcagggcgt ggcgcaggcg gtcgatgtcg cacggggtcg gccgcggcga gtgcatcgtg
     1681 ttgtcctcgc tgtcgtcgtc ggcgcccacg aagccgggcg ggatcggggt cagcgtggtc
     1741 ggcatcagct cggccgggtc ctcgaccttg cggccccaca ggtaggtcag gaggtcgacc
     1801 ccgtcgagca gctccatcgt gaagaagcac gagtccgcgg cgaccacgag gtcgtagagc
     1861 gcgaccaggt tgggatgcga caggtcggcc agcgcgcgga actcgcgctt gagctggtag
     1921 atcttctcga cgtcgaggtt ggagatcgtc ttgagcgcga ccttctggcc gcgctcgacg
     1981 tcgtcggcct cgtacaccac gcccatgccg ccggcgccga tccgccgtac cacctggtag
     2041 cgatcgatgg agctggcgac ctcctgcatc gctcggcctg atgctactcc aactatccgc
     2101 cgcggaggcg ccgcgcgacc tgactcatcg ggtagaccgt ggcgcgaata ccattccgca
     2161 ggccgagtgg ccggaccccg cgaccctgtc ggaacatcga cgcgcgcagc cggcgatcgt
     2221 cgatgtcgat ccgctcggtc agctgcaggt cggcgagcgc gagcgcgccg ctcccggtgt
     2281 cggtcatcag cgtgaacccg gcgcggccgg cggcgatgtc ggcgtcgagc cgcgccagcc
     2341 ggtcgctgcc gcgatccgcc gcggcgggcg gcggggggac cagccgcagg atcaccggcc
     2401 cgtggccggt cagccaccac ggcgtcaccg tcgagtagtg gttcgcgagg tagtcgccgg
     2461 cgtcggtgcg ggcgcgatcg cgcgcggcgg tggcgaacga ctcgaaggtc ccgagcagca
     2521 ggtcctgatc gccgtaggcg gcgtcgtcct tggcgaccgg ctggagccgg acat
//
LOCUS       DASVOR010001139         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_187125,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001139 DASVOR010000000
VERSION     DASVOR010001139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187125"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VF516_21740"
     CDS             <1..355
                     /locus_tag="VF516_21740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8110376.1"
                     /translation="DAVGLGAFAGQRAVELSYGRKRALEIATTLALDPETMLLDEPMS
                     GLGHEDVGRIAALIKSIRANRTIIMVEHNLGVVADLCDRITVLARGEILAEGDYATVS
                     HKPEVIQAYMGTPHA"
     gene            348..1088
                     /locus_tag="VF516_21745"
     CDS             348..1088
                     /locus_tag="VF516_21745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8110377.1"
                     /translation="MPDASAQTAPAAGAAPVLATRNLHAWYGESHILHGMDIEVRSGE
                     LVTLLGRNGAGKTTTLKAIMGMVPRRSGSVRFAGKELIGLPPHRIARLGIAFCPEERG
                     IFASLSVEENLVLPPQVRPGGLSVAAIFELFPNLKERLGSPGTKLSGGEQQMLAIGRI
                     LRSGARFLLLDEPTEGLAPVIVQQIGATIKRLKADGFTILLVEQNIKFAQTVADRHYV
                     VEHGRVVDMVPNADLADSMDRLRVYLGV"
     gene            1184..>2345
                     /locus_tag="VF516_21750"
     CDS             1184..>2345
                     /locus_tag="VF516_21750"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8110378.1"
                     /translation="MTLCAAALCAAACKKPDNAAGSGAASGSTGTAPSGGGAGGDKVV
                     IGVLTDMSGPYADIGGKGSELAAQMAVADFGGKVAGLPIEVISADHQNKPDIGSSIAR
                     EWYDTKGVDTIVDVPTSSVALAITEVTKSKNKVFLVSGAGTSDLTGKACSPNTVHWAY
                     DTWALSNAAGREIIKNGGDTWFFLTVDYAFGAALERDATKVVEQMGGKVLGDVKHPLN
                     AQDFSSFLLQAQSSKAKIIGLANAGKDTINSIKQAAEFGIMAKGQKMAGLLTFITDVN
                     ALGLPAAQGLLLSESFYWDRDDGTRAWSQKFAAKRDGAMPTMVHAGVYSAVMHYLKAV
                     AELKSAKDGKAVVDKMKAMPTDDPLFGKGTIDPNGRKRHSMYVYEVKKPEESK"
BASE COUNT          399 a          802 c          816 g          328 t
ORIGIN      
        1 cgacgcggtc gggctcggcg cgttcgccgg ccagcgcgcg gtcgagctgt cgtacggccg
       61 caagcgcgcg ctcgagatcg cgaccacgct ggcgctcgac cccgagacca tgctgctcga
      121 cgagccgatg tcggggctcg gtcacgagga cgtcgggcgg atcgcggcgc tgatcaagtc
      181 gatccgggcc aaccggacga tcatcatggt cgagcacaac ctcggggtgg tcgccgacct
      241 gtgcgaccgg atcacggtgc tggcgcgcgg cgagatcctc gccgagggcg actacgcgac
      301 ggtgtcgcac aagcccgagg tcatccaggc ctacatgggg acgccgcatg cctgatgcgt
      361 ccgcccagac cgcgccggcg gccggcgccg cgccggtgct cgcgacccgg aaccttcacg
      421 cgtggtacgg cgagtcgcac atcctgcacg gcatggacat cgaggtccgg tccggcgagc
      481 tcgtcacctt gctcggccgc aacggcgccg gcaagaccac gacgctcaag gcgatcatgg
      541 gcatggtgcc gcggcggtcc ggcagcgtcc ggttcgcggg caaggagctg atcggcctgc
      601 cgccgcaccg gatcgcgcgg ctcgggatcg cgttctgccc cgaggagcgc ggcatcttcg
      661 ccagcctgag cgtcgaggag aacctggtgc tgccgcccca ggtccggccc ggcggcctgt
      721 cggtcgcggc gatcttcgag ctgttcccca acctgaagga gcggctgggc agcccgggga
      781 ccaagctgtc gggcggcgag cagcagatgc tggcgatcgg ccgcatcctg cgcagcgggg
      841 cccggttcct cctgctcgac gagccgaccg aggggctggc cccggtgatc gtccagcaga
      901 tcggcgcgac catcaagcgg ctcaaggccg atggcttcac gatcctgctg gtcgagcaga
      961 acatcaagtt cgcgcagacc gtggcggacc gccattacgt ggtggagcat ggccgggtcg
     1021 tcgacatggt ccccaatgcc gatctcgcgg acagcatgga ccgactgcgc gtgtaccttg
     1081 gagtctagcc ccccggccag gccgcctggc cgatcccaac catcgtgaca tgaacacccg
     1141 aggagctgcg atgctgagac acctggcgaa tcgttcgatg atcgtgactc tgtgtgccgc
     1201 ggcgctgtgc gcggcggctt gcaagaagcc cgacaacgcg gccggcagcg gcgcggcatc
     1261 gggctcgacg ggcaccgccc cgtcgggcgg cggggccggc ggcgacaagg tcgtcatcgg
     1321 cgtcctgacc gacatgtccg ggccgtacgc cgacatcggc ggcaagggct ccgagctcgc
     1381 cgcgcagatg gcggtcgcgg acttcggcgg caaggtcgcc ggcctgccga tcgaggtgat
     1441 ctccgccgac caccagaaca agcccgacat cggctcgagc attgcccgcg agtggtacga
     1501 caccaagggc gtcgacacca tcgtcgacgt gccgacctcg tcggtcgcgc tcgcgatcac
     1561 ggaggtcacc aagagcaaga acaaggtgtt cctggtgtcc ggcgccggca cctcggacct
     1621 gaccggcaag gcgtgctcgc ccaacacggt gcactgggca tacgacacct gggcgctgtc
     1681 caacgccgcc gggcgcgaga tcatcaagaa cggcggcgac acctggttct tcctcaccgt
     1741 cgactacgcg ttcggcgcgg cgctcgagcg cgacgcgacc aaggtcgtcg agcagatggg
     1801 tggcaaggtg ctcggggacg tcaagcaccc gctcaacgcg caggacttct cgtcgttcct
     1861 gctccaggcc cagtcctcga aggccaagat catcggcctg gccaacgcgg gcaaggacac
     1921 gatcaactcg atcaagcagg ccgccgagtt cggcatcatg gccaagggcc agaagatggc
     1981 gggcctcctg acgttcatca ccgacgtcaa cgcgctcggc ctgccggccg cgcagggcct
     2041 cctgctcagc gagtcgttct actgggatcg cgacgacggc acccgcgcgt ggtcgcagaa
     2101 gttcgcggcc aagcgcgacg gcgcgatgcc gaccatggtc cacgccggcg tgtactcggc
     2161 ggtgatgcac tacctcaagg cggtcgccga gctcaagagc gccaaggacg gcaaggcggt
     2221 ggtcgacaag atgaaggcga tgccgaccga tgatccgctg ttcggcaagg gcacgatcga
     2281 tcccaacggc cgcaagcggc actcgatgta cgtctacgag gtcaagaagc ccgaggagtc
     2341 gaagg
//
LOCUS       DASVOR010001140         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_449751,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001140 DASVOR010000000
VERSION     DASVOR010001140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449751"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..313)
                     /locus_tag="VF516_21755"
     CDS             complement(<1..313)
                     /locus_tag="VF516_21755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX8110379.1"
                     /translation="MTERCEDLDLFFDRELEPEAEHAYREHLAGCSRCQEVLLGRMLE
                     ATVVSEDRDSRPSIDPIPTVGVDGAAPPPDRAPERTIGPPSGPPPPSDRPTRTTCCRT
                     RS"
     gene            complement(310..807)
                     /locus_tag="VF516_21760"
     CDS             complement(310..807)
                     /locus_tag="VF516_21760"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8110380.1"
                     /translation="MLEDAAPILTRIAQRLYGNTPDAHDLLQDTIERAIRQGLPADVQ
                     SPRAWLATIMHNLFVDRCRAAARAPVHEALEEFHDNVTPLSVDSPEPAWSQLTIEDVR
                     AALEAIDRPFREVYVMHTFERRSYEEIAALLQISRVTVGTRLTRTRKMLRKELVKRFG
                     RGKKP"
     gene            complement(1241..>2168)
                     /gene="ligD"
                     /locus_tag="VF516_21765"
     CDS             complement(1241..>2168)
                     /gene="ligD"
                     /locus_tag="VF516_21765"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8110381.1"
                     /translation="PPPVELEVERPVRELRLSNLQKVFWKSEAITKGDLIDYYRAIAP
                     HILPYLANRPAVLTRYPDGIDGEMFYQKDLPGWAPPWLRTTVRWSEHSQREIHYVLID
                     DADGLAFVANLGAIQLHAWASRIIDLEKPDWTILDLDPKNAPREHVVPLALAIHALCD
                     AAGLPNYVKTSGQSGLHVLIPLGGQCTFEQARMLAYLIGQLVERRHPDMATTARNPAA
                     RRGRVYLDWGQNAHGQLLVVPFSVRPVPGAPVSMPLVWDEVVPGLDARQFHLRNALDR
                     VTAWPGDPCLPVLTERPDLAAALARLEQVAQS"
BASE COUNT          304 a          717 c          822 g          325 t
ORIGIN      
        1 tcgatcgtgt cctgcagcag gtcgtgcgcg tcgggcgatc ggacggcggc gggggcccgg
       61 acggaggccc gatcgtccgc tccggggcgc ggtcgggcgg cggtgcggcg ccgtcgacac
      121 ccacggtggg gatcgggtcg atcgacggcc ggctgtcgcg gtcctcgctg accacggtgg
      181 cctccagcat ccgccccagc aggacctcct ggcagcgcga gcagccggcg aggtgctcgc
      241 gataggcgtg ctcggcctcg ggctccagct cgcgatcgaa gaacagatcg agatcttcgc
      301 agcgctcggt cacggcttct tcccccgtcc aaatcgcttc acgagctcct tgcgcagcat
      361 cttgcgcgtg cgggtcagcc gggtcccgac cgtgacccgg ctgatctgca acagcgcggc
      421 gatctcctcg tacgaccgcc gctcgaacgt atgcatcacg tagacctcgc gaaacggccg
      481 atcgatcgcc tcgagcgccg ctcgcacgtc ctcgatcgtc agctggctcc acgccggctc
      541 gggactatcg acgctgagcg gcgtgacgtt gtcgtggaac tcctccagcg cctcgtgcac
      601 cggggcgcgc gccgcggccc ggcagcgatc gacgaacagg ttgtgcatga tcgtcgccag
      661 ccacgcgcgc ggactctgca cgtcggcggg cagcccctgg cggatcgcgc gctcgatggt
      721 gtcctgcagc aggtcgtggg cgtccggcgt gttgccgtac agccgctgcg cgatccgggt
      781 gaggatcggt gcggcatcct ctagcaccgt gtggagggtc gtggctttgc ctgacacgtg
      841 ctgtaccgga tcagccgacc catcggcagg ctggcacgcc ggtaaatagc cggtcaaggt
      901 cacgatatct gggggacgac cgcaggatgg gacattccca aaaaacgtcg acgcgagcgc
      961 cgagcgcgcg cggggcgcgg ccgaaaatga cggcctcttc cgtcggcagt tggtagcgcg
     1021 gctcacggag cttccggtgc ctgacgtcgc cgcgcgcgag gccaaaaaaa acgggaatgc
     1081 gcggcgcggg catcgtcacc cagctgcgga ggatggtcac gggtcgcggg gagaagtccg
     1141 tcgccggcgg tcgtggtgcg tggtcgggat ggtggcgccg gcgggatggt ggcgtcggcg
     1201 gcggacttcg cgggtggcgg tgagcggagc gcgcggcgcg ctacgactgc gcgacctgct
     1261 ccagccgcgc gagcgccgcc gcgagatcgg ggcgctcggt caagaccggc aggcacggat
     1321 cgccgggcca cgcggtgacc cggtcgagcg cgttgcggag atggaactgc cgggcgtcga
     1381 gcccggggac gacctcgtcc cagaccaggg gcatcgacac cggcgcgccg ggcaccggcc
     1441 ggacgctgaa cggcacgacc agcagctggc cgtgcgcgtt ctggccccag tcgaggtaga
     1501 cccggccgcg gcgcgcggcg gggttgcgcg cggtggtcgc catgtcgggg tggcggcgct
     1561 cgacgagctg gccgatcagg tacgccagca tgcgcgcctg ctcgaacgtg cactggccgc
     1621 cgagcgggat caggacgtgg agcccgctct ggcccgacgt cttgacgtag ttgggcagcc
     1681 cggcggcgtc gcacagcgcg tggatcgcga gcgcgagcgg cacgacgtgc tcgcgcggcg
     1741 cgttcttcgg atcgagatcg aggatcgtcc agtccggctt ctcgaggtcg atgatccggc
     1801 tcgcccaggc gtggagctgg atcgcgccca ggttggcgac gaacgccagg ccgtcggcgt
     1861 cgtcgatcag cacgtagtgg atctcgcgct ggctgtgctc gctccaccgc acggtggtgc
     1921 gcagccacgg cggggcccag cccggcaggt ccttctggta gaacatctcg ccgtcgatgc
     1981 cgtcggggta gcgggtcaag acggccggcc ggttggcgag gtagggcagg atgtgcggcg
     2041 cgatcgcgcg gtagtagtcg atcaggtcgc ccttggtgat ggcttcggac ttccagaaca
     2101 ccttctggag gttcgacagc cggagctcgc ggaccgggcg ctcgacctcg agctcgaccg
     2161 gcggcggc
//
LOCUS       DASVOR010001141         6450 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420021,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001141 DASVOR010000000
VERSION     DASVOR010001141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420021"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..630
                     /locus_tag="VF516_21770"
     CDS             166..630
                     /locus_tag="VF516_21770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110382.1"
                     /translation="MTTTTIEVPTSCAALCTKLAELAQRIDDEIAQAGSGAAVDYGAL
                     EQRVADALGELERGLHAQVLARLDVNVPAIRVWGDEYRRIGRAESDYHTLAGTVRVMR
                     TVYRKTERNGATLDPVSVRAGVVADGWLPYAARAMAHLIAQGTSREARGDGA"
     gene            1110..1733
                     /locus_tag="VF516_21775"
     CDS             1110..1733
                     /locus_tag="VF516_21775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110383.1"
                     /translation="MAYLTDGAVEFETLYDQHLKLPLGPDVVSLVDFWHAAEYLGAAA
                     RVFEVKRKAKPGQFRRWRHALKHDAGAAAAIVEAMEQSGLEQVMLDGTRPVEAALRYF
                     RARLARMDYAGARSAGLPIGSGNVEATCKSLVSCRMKRPGARWKHASGDDVLQLRALQ
                     LSDRWAPAITRATKPLAKPVQIAAGVHVVKSARTAAVTSAHGARAAA"
     gene            complement(1978..2115)
                     /locus_tag="VF516_21780"
     CDS             complement(1978..2115)
                     /locus_tag="VF516_21780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110384.1"
                     /translation="MSGGEVIGLVAQHSGRDLAAGAGRRIAGDLVEVAHRGLLIESLS
                     T"
     gene            complement(2246..2431)
                     /locus_tag="VF516_21785"
     CDS             complement(2246..2431)
                     /locus_tag="VF516_21785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110385.1"
                     /translation="MPAPAVAIAAATAWWRPSNPMTAAGSGRDELFAVAHVAGVDLVE
                     VARGRIRGGGSGGGPSC"
     gene            2728..3570
                     /locus_tag="VF516_21790"
     CDS             2728..3570
                     /locus_tag="VF516_21790"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110386.1"
                     /translation="MARAQPRFEFGSLPRDPDRDAALVDVREPEHQREEIVLSDDLTR
                     KLDRTIAEFRNRDTLAKHALVPRSRLLFVGPPGCGKTLAADVLAAELSLPLVYARFDG
                     IVSSFLGETATNLRRVFSYAAKAPSLLFFDEFDTIGKRRDDDHEVGELKRVVGSFLQI
                     LDSYPRDQLVVAATNHEGLLDEALWRRFDDVLVFRRPMVEQLGELLTMKLRGVRKTGV
                     DVHALAAEMAGFTFADAERVCVEAIKSMVLSGAKELRVDLLRAELDEQRARLRDAGTV
                     IASE"
     gene            3588..5951
                     /locus_tag="VF516_21795"
     CDS             3588..5951
                     /locus_tag="VF516_21795"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX8110387.1"
                     /translation="MADGDQYPLLRLGAPRKLPRKKRNPNFPPPPPRDVNRHGRALLA
                     GADLARQRVADAAAISRDLATDVPYVRIQHDPRNMVTDEGLVRMGLVPVMHRQDHVIA
                     AYATDAQLGTLRKKVGEYQRQTAKHGTLSSIATLGPWTRDDRTSPRLKELSIARDGSY
                     TVDLMFLPIDGARVAPGARDAVTRFVQANGGKVLDQIRSARFEALRVRLGGQSLDLLL
                     EHRADVALVDLPPRAHISVPAVLSFSIDDMPDPQPLNDSTPMVCVIDSGIVEGHPLLA
                     HTIAEKHSKAYPAALGPPIPGAGTDAARHGTGVAGVTLYGDIARCVTSKTFEPAASLI
                     NARILDDNIELDPERMPYVRQIVDDVGSICRIFNLSLGLEPAGTALSSYASDLDALAR
                     ERDAIFIVSAGNEPPWIKYPDPTQLPDHPKYLLEEGMAVRQPAEALNVLTVGSITTAN
                     DPHPPSTSIRGAAGKRASSPFTRAGAIRNVVKPDLVEEGGEVGIDTQLNRWALSNPGL
                     RIPTTGPEFATGKVFTYTDGTSIAAPKVAYFVARILGVMPDASANLVRALLVNSAGFP
                     EGAQTYPLAQAMRLCGFGVPNLNRALFCTDARATAYFAGEIEIDDVLMFDIHVPSELT
                     ESKGTKRITVTVTYDPPVSALEQLRPAGINLTWKVAKANVAESVIEAELVAAAEQELA
                     GTADDEPKEKKKSKKKVFEAGKLPRRAQQRGTVQKNIFEWTRKVPGDTWRLALTAKAT
                     RPAHANEKQRFAVAVTIEHVTEPVALYQAIRARLPAGRVRVRVPASR"
     gene            complement(5866..>6450)
                     /locus_tag="VF516_21800"
     CDS             complement(5866..>6450)
                     /locus_tag="VF516_21800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110388.1"
                     /translation="ARGRWIGPLPMRYHGTRAPLGWTWSDPAARFTRIHTLADGTGGE
                     PVERWATAHGAAMVDVIATGLDDGCAGANSTRRWRRSRPGRQWLALSTEERLRVLRIT
                     YYENMRQFFRGFGKAAKDAERSAERALRRVPGGAAAVEARDGHGAEYRAVPIGSNYPE
                     ATDGWPINATPEPEHERGRRGGEHGSPDTRRRVR"
BASE COUNT         1050 a         2259 c         2200 g          941 t
ORIGIN      
        1 gtggaggaac gcagcaacgt gtttttcaag tcgttcgtgg ttgagcatca tcctcaattg
       61 tagttcacgc acaaagagac catcggttcg gccagaagct tgccttccgt cgcgtcgagg
      121 atctgaatgg gcgcgcgtcg actcgacgac gcggggagcc caccgatgac taccacgacg
      181 atcgaggtgc cgacgagctg tgcggcgctg tgcaccaagc tggcggaact ggcccagcgc
      241 atcgacgacg agatcgccca ggcgggtagc ggtgcggcgg tcgactatgg cgcgctcgag
      301 cagcgtgtgg ccgacgcgct cggcgagctc gagcgcgggc tgcacgcgca ggtgctcgcg
      361 cggctcgacg tcaatgtgcc agcgatccgg gtgtggggcg acgagtaccg gcggatcggc
      421 cgggccgaga gcgactacca cacgctcgcg gggacggtgc gggtgatgcg gacggtgtac
      481 cggaagacgg agcgcaacgg cgccacgctc gatccggtga gcgtgcgcgc cggcgtggtc
      541 gccgacgggt ggttgccgta cgcggcgcgg gcgatggcgc acctgatcgc gcaggggacc
      601 tcgcgcgagg cgcgaggcga cggcgcgtga gctggtgcgg ttgccgtact cgcgctcgag
      661 cttcgagcgc gtcggccacg aggtcggcac gttggtgcga cgggcgcagc cgcggatcga
      721 ggaggcgctg atccgcgagt acgcggtgcc cgccgaagcg cgcagcgcga gcatctcgat
      781 cgaccgggtc gcgatgccga tggaggaacc gatcgaggcg ccgccggtac cggtggatcc
      841 gccgtgggtc gctgcggtgc tggccgccgc gccgccgcat ccgcccgagg tgcaggcggt
      901 gatcgacgag cagaaccaag gcgacgcggc caaggtcgcg cgcaacttcc gcatggccta
      961 cgtggcgacg gtgacgctgc acgacgccga cggcgaggcg atccatacaa tccggtatgg
     1021 ccggatgccc aagggcgacg cgcgcggact gtgccgcggc ctggcgcgcg acgtcacggc
     1081 gatgcgagcc ccagcggccg gatctcaagg tggcgtacct gaccgatggc gccgtcgagt
     1141 tcgagacgct gtacgaccag cacctcaagt tgccgctcgg tccggacgtc gtgtcgctcg
     1201 tcgacttttg gcacgcggcc gagtacctcg gcgccgcggc ccgggtgttc gaggtcaagc
     1261 gcaaggccaa gcctggccag ttccggcgct ggcgacacgc gctcaagcac gacgccggcg
     1321 ccgcggccgc gatcgtcgag gcgatggagc agagcgggct cgagcaggtg atgctcgatg
     1381 gcacgcggcc ggtcgaggcg gcgcttcggt acttccgcgc gcgcctggcg cgcatggact
     1441 acgcaggcgc gcggagcgcg ggcttgccga tcggcagcgg caacgtcgag gcgacctgca
     1501 agagtctagt gtcgtgccgg atgaagcggc cgggcgcgcg gtggaagcac gcgagcggcg
     1561 atgacgtgtt gcaactccgc gcgctccagc tcagcgaccg ctgggcgccg gcgatcacgc
     1621 gcgcgaccaa gccgctcgcc aagcccgtgc agatcgcggc cggcgttcac gtggtgaagt
     1681 cggcccggac tgcggcggta accagcgcgc acggggcacg cgccgcggcg tagccggccc
     1741 agccagcggt accgcgcgcg ggcgcgaaaa cggctccgac gaggatcggg gccgtaacgg
     1801 cggattatgc aaggaagtca cagtattaga accacacccc cggcacagtt gacagtccgt
     1861 aaattctgcg acccaaactc tgaatcggag aacggggcag ggagttcaga attgtaatat
     1921 acacgtgcgc tcttttcgtc gcgagccgcg cccccggctc gccgagctgg tcttcgctca
     1981 cgtcgacagg ctctcgatga gcaagccgcg gtgcgcgacc tcgaccaggt cgccagcaat
     2041 gcgcctgcca gcgccagcag ccagatcgcg gccgctatgc tgcgcgacaa ggccgatgac
     2101 ctccccgcca ctcatgtcgt gaacgtccgc gctcgcggtg agctcccccg cctcggcggg
     2161 tgctacggtc gcccccgaga cggatgtgac ggcgctgagc gccggtgacg cgaaggtgtc
     2221 gccggcgggg acttgggcgc gggcgtcagc aggacggccc gccgcccgag ccgccaccgc
     2281 gaatgcgtcc gcgcgcgacc tcgaccaggt cgacgccggc gacgtgcgcc accgcgaaca
     2341 gctcgtccct gcccgacccc gccgctgtca tcgggttcga cggccgccac cacgccgtcg
     2401 cagcagcgat cgccacggcc ggggccggca ccgcgaccgt acgtgcgcga cctcgaccac
     2461 tagccgctcg cccgaggtcc ggccgtcgcc gagcttgatg ttctcgccgc gtcccgccca
     2521 gcagcgcgcg cgaccagcgc cgagcgcata tgacggtact gcgggcgtct gacaggacgg
     2581 ccgggtggat ttcgtagctt ctacggctct cctcaaaagg tcgatgtttc agtggcttgg
     2641 cgatcctttc gcggtcctat cagggcccat ggcaagatcg gcgaagcttg gctgacgatc
     2701 agcccggcat gagacaagaa tcggctcatg gcacgcgcgc aaccccggtt cgagttcgga
     2761 tccctgccca gggaccccga tcgggacgcc gcgctcgtcg atgtccgcga gcccgagcat
     2821 cagcgggagg agatcgtcct ctccgatgac ctcacccgca agctcgaccg cacgatcgct
     2881 gagttccgta accgcgacac gctcgccaag cacgcgctgg tgccgcggag ccggctgttg
     2941 ttcgtcgggc cgccggggtg cggcaagacg ctcgcggccg acgtgctcgc cgccgaactg
     3001 tcgctccccc tggtctacgc gcggttcgac ggcatcgtgt cgtcgttcct cggcgagacc
     3061 gcgaccaacc tccgccgggt attcagctac gcggccaagg ccccgtcgct gctgttcttc
     3121 gacgagttcg acacgatcgg caagcggcgc gacgacgatc acgaggtcgg cgagctgaag
     3181 cgggtggtcg gcagctttct ccagatcctc gattcctacc cgcgtgatca gctcgtcgtc
     3241 gccgcgacca accacgaggg cctcctcgac gaggcgctgt ggcgccgctt cgatgacgtc
     3301 ctggtgttcc gtcggcccat ggtcgagcag ctcggcgagc tgctcaccat gaagctgcgc
     3361 ggggtgcgca agaccggcgt cgacgtccac gcgctcgccg ccgagatggc aggcttcacg
     3421 ttcgccgacg ccgagcgcgt gtgcgtcgag gccatcaagt cgatggtgtt gtccggcgcc
     3481 aaggaacttc gcgtcgatct gctacgtgcc gagctggacg agcaacgcgc ccggctgcgc
     3541 gacgccggga ctgtcattgc ctcggagtaa cgtagccgtc ggttgatatg gccgacggtg
     3601 atcagtaccc gctgctgcga ttgggcgcgc cgcgcaagct gccgcggaag aagcgcaatc
     3661 ccaacttccc gccgccgccc ccgcgcgatg tgaaccgcca cgggcgcgcg ctgttggcag
     3721 gagccgacct ggcacggcag cgcgtcgccg acgcggctgc gatctcgcgt gatctcgcga
     3781 ccgatgtccc gtacgtccgg atccaacacg acccgcgaaa catggtgacc gacgaggggc
     3841 tcgtgcggat gggactcgtg ccggtgatgc atcgccagga tcacgtgatc gccgcgtacg
     3901 ccaccgatgc tcagctcgga acgctccgca agaaggtcgg cgagtatcag cgccagaccg
     3961 ccaagcacgg cacgctgtcg tccatcgcga cgctcgggcc gtggacccga gacgatcgaa
     4021 cgtcgccgag gctcaaagag ctgtcgatcg cgcgcgacgg ttcgtacacc gtcgacctca
     4081 tgttcctgcc gatcgacggc gcgcgcgtgg cgccgggggc gcgggacgcc gtcacgcgct
     4141 ttgtccaggc gaacggcggc aaggtgctcg accagatacg ttcggcgcgg ttcgaggccc
     4201 taagggttcg cctgggcggc cagtcgctcg acctgctgct cgaacaccgg gccgacgtgg
     4261 cgctcgtcga ccttccgccg agagcgcaca tctccgtccc tgcggtgctc agcttctcga
     4321 tcgacgacat gcccgacccg cagccgctca acgactcgac accgatggtg tgcgtgatcg
     4381 actcgggcat cgtcgaaggt cacccgcttc tcgctcacac gatcgccgag aagcactcga
     4441 aggcgtaccc tgcggcgctc ggcccgccga tccccggcgc cgggaccgat gccgcccgtc
     4501 acgggaccgg cgtcgctggt gtcacgctgt acggcgacat cgcacgctgc gtgacctcca
     4561 agacgttcga gccggccgca tccctgatca acgccaggat cctcgacgac aacattgagc
     4621 tggatccaga acggatgccc tacgtccgcc agattgtcga cgacgtcggc agcatctgcc
     4681 gcatcttcaa tctttcgctc ggcctggaac cagcgggcac ggcactgtcg tcctacgcat
     4741 cggatctcga tgccctggcc cgcgagcgcg acgcgatctt catcgtgtcc gccggcaacg
     4801 agccgccgtg gatcaagtac ccggatccga cgcagctccc cgaccacccc aagtaccttc
     4861 tcgaggaagg catggcggtc cgccaacccg ccgaggcgct caacgtgctg accgtgggca
     4921 gcatcacgac cgcgaacgat ccccatcccc cgagcacgtc gatccgcgga gccgccggca
     4981 agcgtgcatc gtctccgttc acgcgcgccg gcgcgattcg caatgtcgtc aagcccgacc
     5041 tcgtcgagga gggcggcgag gtcggcatcg acacgcagct caaccgctgg gcactttcga
     5101 accccggcct tcgcatcccg acgaccggcc ccgagttcgc gaccggcaag gtgttcacgt
     5161 acaccgacgg aaccagcatc gccgcgccga aggtcgccta cttcgtcgcg cgcatcctcg
     5221 gcgtgatgcc cgacgcatcg gcgaacctgg tccgggcgct gctcgtgaac tcggcggggt
     5281 tcccggaggg cgcgcagacc tatcccctag cacaagcgat gaggctgtgc gggttcgggg
     5341 tgccgaacct caatcgcgcg ctgttctgca ccgacgcacg tgcgaccgcg tacttcgcgg
     5401 gagagatcga gatcgacgac gtcctgatgt tcgacatcca cgtgccgtcc gagctgaccg
     5461 aaagcaaggg cacgaagcgg atcacggtca cggtcaccta cgatccgcca gtgtcggcgc
     5521 tcgaacagct ccggccggcc gggatcaacc tgacgtggaa ggtcgcgaag gccaacgtcg
     5581 ccgagagcgt catcgaggcc gaattggtcg ccgccgccga gcaagagctg gcgggcacgg
     5641 ccgacgacga gcccaaggag aagaagaaat ccaagaagaa agtcttcgag gcggggaagc
     5701 tacctcgacg cgcgcagcaa cgaggcacgg tccagaagaa catcttcgag tggacccgca
     5761 aagtcccagg tgacacctgg cgcctcgcgc tgaccgccaa ggcgacacgc ccggctcacg
     5821 cgaacgagaa gcagcggttc gctgtcgccg tcacgatcga gcacgtcacc gaacccgtcg
     5881 ccttgtatca ggcgatccgt gctcgcctcc ccgccggccg cgttcgtgtt cgggttccgg
     5941 cgtcgcgttg atcggccaac catccgtcgc ttccggatag ttcgaaccga tgggaaccgc
     6001 ccggtattcg gcgccatgcc cgtcgcgcgc ttccacggcg gcggcgccgc cggggacccg
     6061 gcgcagcgcc ctctccgccg agcgctcggc gtccttggcc gccttgccga agccgcggaa
     6121 gaactgccgc atgttctcgt agtacgtgat ccgcaagacg cggagccgct cctcggtcga
     6181 cagcgccagc cactggcgac cgggcctgct ccgccgccac cgccgcgtcg agttcgcgcc
     6241 ggcacacccg tcatcgagcc cggtcgcgat cacgtcgacc atcgccgcgc cgtgcgccgt
     6301 cgcccaccgc tcgaccggct cgccgcccgt gccgtccgcc agggtgtgga tccgggtgaa
     6361 gcgcgccgcg gggtcgctcc aggtccaacc cagcggagcg cgggtgccgt ggtagcgcat
     6421 cggcagcggc ccgatccagc ggccgcgagc
//
LOCUS       DASVOR010001142         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_273863,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001142 DASVOR010000000
VERSION     DASVOR010001142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273863"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            99..422
                     /locus_tag="VF516_21805"
     CDS             99..422
                     /locus_tag="VF516_21805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110389.1"
                     /translation="MSRLADIASAVTDGIVAAWVDTRTGALLEQHVVRDGPLVAHARD
                     AAAEVMRSRERPPRMVLMSAHHIHIVQRVAHEPRRALVVVCERSPNIGLAVALVRAFA
                     AAGPA"
     gene            419..1225
                     /locus_tag="VF516_21810"
     CDS             419..1225
                     /locus_tag="VF516_21810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110390.1"
                     /translation="MMQGSTLGVEVLHLLEQVPCNASGALVRERSDGTMAGALLVEHG
                     RVCWAMSQRYRIRLTDLLISEQSTLSGEQLDEVVALCQRDHLPLGETLLTRGLVSLPV
                     LHRALLRHTCEALDCLVRDDASPWSWVEHSGYGYHPILTFSAAEILTGVRAIGNPPLA
                     ARALARLRDVVRSGQLGFAIERTPGAKLPLAQLGCEHVDLAALADLAAQADEVMAVAA
                     TVDLDAAIMELDGLACASWIEDGVLFVVLCDGELAFNRLLAQVAAMTTRT"
     gene            1266..1598
                     /locus_tag="VF516_21815"
     CDS             1266..1598
                     /locus_tag="VF516_21815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110391.1"
                     /translation="MEIDGIIGACVVDSNSGMMLGAVGGGSQLNLELAAAGNTEVVRS
                     KRKTIKSLNLNDPIEDILITLGRQYHLMRPLTSNDALFIYLALDRSRANLALARHYLA
                     SAEKELVV"
     gene            1623..1991
                     /locus_tag="VF516_21820"
     CDS             1623..1991
                     /locus_tag="VF516_21820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110392.1"
                     /translation="MPAEKQIIESFEKIANEIPGFIGASLVDLESGMTLGTKSVRGDF
                     DLAAASAYNSEMVKQKQKIMRALSLKTSLEDMLITLGDQIHLIKLVSPTTFIYLAADR
                     ATANLAIVRVAVNKHIGLFS"
     gene            1997..>3424
                     /locus_tag="VF516_21825"
     CDS             1997..>3424
                     /locus_tag="VF516_21825"
                     /inference="COORDINATES: protein motif:HMM:NF012255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX8110393.1"
                     /translation="MAVLDADHNAVVIRIVYDGPPHAGKTTSLRALGRSLGRSVEAPG
                     EIAGRTVYFDWMEYTGGLFEGQQIRCQIVSVPGQRHLAARRRALLDTADVVVFVADTS
                     DRAAVDASVGHVRQMVDILRGLGDPPVGVVVQANKRDLPGAVPRDELRAALGEDFART
                     ALTESIAEAGEGIRETFVLAVRLALDRIRELMAQGQLPRGRPAIDTSEQLLAALERQP
                     APGDIASAVMSIVPAARPRLPDARVPSGAIWPPVEGRLVLHEATAGELALHRVDSGDW
                     LAGIGSGWRLHSAASAAFAEFDDGRQALLAWARLHAAHVELLSPSRCVVLAAADNSTW
                     RLWQIVKLAPPLRAWLVQAAALDASELLARLATAAATLGEAHARCADNRLLPTLDTIG
                     QGVRGAQFVALMPGPSAAPLPRPTDVGGYIARELARMLAADLVERRSELAQRVTHRGT
                     SPTSWDDVVAAAIADRVPPPPADMAP"
BASE COUNT          505 a         1218 c         1202 g          499 t
ORIGIN      
        1 gagcgcggcg agctgatcgt catggcggcg ccggctgcgc tatcggtcgc cctgagctgg
       61 gcgctggtcc gcacgctggc cgccggggag gccggggcgt gagtcggctg gccgacatcg
      121 ccagcgcggt caccgacggc atcgtcgcgg cctgggtcga cacccggacc ggcgcgctgc
      181 tggagcagca tgtcgtgcgc gacggcccgc tcgtcgcgca cgcgcgcgac gcggcggccg
      241 aggtcatgcg ctcgcgcgag cgcccgccgc gcatggtgct gatgtcggcg caccacatcc
      301 acatcgtcca gcgcgtggcg cacgaaccgc gccgcgcgct ggtcgtggtc tgcgagcgat
      361 ctccgaacat cgggctcgcg gtcgcactgg tccgggcgtt cgccgcggcg gggccggcgt
      421 gatgcagggc tcgacgctcg gcgtcgaggt gctgcacctg ctggagcagg tgccgtgcaa
      481 cgccagcggc gcgctggtgc gcgagcgctc cgatggaacc atggccggtg cgttgctcgt
      541 cgagcacggc cgggtgtgct gggcgatgag ccagcgctac cggatccgcc tgaccgacct
      601 cctgatctcg gagcagagca cgctgtctgg cgagcagctc gacgaggtgg tcgcgctgtg
      661 ccagcgcgac cacctgccgc tcggcgagac cctgctcacc cgcggcctgg tgtcgctccc
      721 cgtgctgcac cgtgcgctgc tgcgccacac ctgcgaggcg ctcgattgcc tggtccgcga
      781 cgacgcctcg ccgtggtcgt gggtcgagca cagcggctac ggctatcacc cgatcctgac
      841 gttctcggcg gccgagatcc tcaccggggt ccgcgcgatc ggcaacccgc cgctcgccgc
      901 tcgagcgctg gccaggctgc gcgacgtggt ccgcagcggt cagctcgggt tcgcgatcga
      961 gcgcacgccc ggcgccaagc tgccgctggc gcagctcggc tgcgagcacg tcgatctggc
     1021 cgcgctcgcc gacctcgcgg cccaggccga cgaggtgatg gcggtcgcgg ccacggtgga
     1081 cctcgacgcc gcgatcatgg agctcgacgg actggcctgc gcgagctgga tcgaggacgg
     1141 cgtgctgttc gtggtgctgt gcgacggcga actggccttc aaccgactgc tggctcaggt
     1201 ggcagcgatg acaacccgaa cctaggagac ctatggcgac cgcaaaagac gtgttgacaa
     1261 aactgatgga gatcgacggc atcatcggcg cttgcgtcgt cgacagcaac agcggcatga
     1321 tgctgggagc agtcggtggc ggatcacagc tcaacctgga gctggcggcc gccggaaaca
     1381 ccgaggtcgt gcgctcgaag cgcaagacga tcaagtcgct caatctcaat gatccgatcg
     1441 aggacatcct gatcactctc ggtcgccagt atcatctgat gcgcccgctg accagcaacg
     1501 acgcgctgtt catctacctc gcgctcgacc gtagccgcgc gaacctggcg ctcgcccgcc
     1561 attacctggc gagcgccgaa aaggaactcg tcgtctaaac gcccctgcac aggagaccta
     1621 tcatgccggc agaaaaacag atcatcgaaa gtttcgagaa gattgccaat gagatcccgg
     1681 gcttcatcgg agcctcgctg gtcgatctcg aatccgggat gacgctgggc accaagtcgg
     1741 tgcgcggcga cttcgacctc gcggcggcga gcgcctacaa cagcgagatg gtcaagcaga
     1801 agcagaagat catgcgtgcg ctgtcgctca agaccagcct ggaggacatg ctgatcacac
     1861 tcggcgacca gatccacctg atcaagctgg tgtcgcccac cacgttcatc tacctcgcgg
     1921 cggaccgtgc gaccgcgaac ctcgcgatcg tccgggtcgc ggtcaacaag cacatcgggc
     1981 tgttctcgta gctgccatgg cggtcctcga cgccgaccac aacgccgtcg tcatccgcat
     2041 cgtctacgac gggccgccgc acgcaggcaa gaccaccagc ctgcgcgcgc tcggccgtag
     2101 cctcggccgc agcgtcgagg caccgggcga gatcgcgggg cgcaccgtgt acttcgactg
     2161 gatggagtac accggtgggc tgttcgaggg ccagcagatc cggtgccaga tcgtgagcgt
     2221 gcccggccag cgccacctgg cggcccgtcg ccgggcgctg ctcgacaccg cagacgtcgt
     2281 ggtgttcgtc gccgacacca gcgatcgcgc ggcggtcgac gccagcgtcg gtcacgttcg
     2341 ccagatggtc gacatcctgc gcggactcgg cgatccgccg gtcggcgtgg tcgtgcaggc
     2401 caacaagcgc gatctgcccg gcgcggtccc gcgcgacgaa ctgcgggcgg cgctcggcga
     2461 ggacttcgcg cgaacggcgc tgaccgagtc gatcgccgag gccggcgaag ggatccgcga
     2521 gaccttcgtg ctcgcggtcc ggctcgcgct cgatcggatc cgcgagctga tggcgcaggg
     2581 tcagctcccg cgcggcaggc cggcgatcga cacctcggag cagctgctcg cggcgctcga
     2641 acgccagccg gcgccgggcg acatcgcgtc cgcggtgatg tcgatcgtgc ccgcggcgcg
     2701 cccgcgcttg ccggacgcgc gcgtgccgag cggtgcgatc tggccgccgg tcgaaggccg
     2761 cctggtcctg catgaggcga ccgccggcga actcgcgctc caccgggtcg actccggcga
     2821 ctggctcgcc gggatcggca gcgggtggcg cctgcactcg gcggcctccg ccgcgttcgc
     2881 cgagttcgac gacggccgcc aggcgctgct cgcgtgggcg aggctccatg ccgcacacgt
     2941 cgagctgctg tcgccgtcgc gctgcgtcgt gctcgccgcg gccgacaaca gcacatggcg
     3001 gttgtggcag atcgtcaagc tcgcgccgcc gctgcgcgcg tggctggtcc aagccgccgc
     3061 gctcgacgcc agcgagctgc tcgctcggct cgcgaccgcg gcggcgaccc tcggcgaggc
     3121 ccatgcacgg tgcgccgaca accgcctgct gcccacgctc gacacgatcg gacagggcgt
     3181 gcgcggtgcg cagttcgtcg cgctcatgcc tggaccatcg gccgcgccgt tgccgcgtcc
     3241 caccgacgtc ggcgggtaca tcgcgcgcga gctggcccgg atgctcgccg ccgatctggt
     3301 cgagcggcgc tccgagctcg cgcagcgagt cacccaccgc gggacctcgc cgacatcatg
     3361 ggacgacgtc gtcgcggctg cgatcgcgga ccgcgtgcca ccaccgccgg cggacatggc
     3421 accg
//
LOCUS       DASVOR010001143         5618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_245465,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001143 DASVOR010000000
VERSION     DASVOR010001143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5618
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245465"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1204
                     /locus_tag="VF516_21830"
     CDS             <1..1204
                     /locus_tag="VF516_21830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110394.1"
                     /translation="KALALLLTALQGLNATLFKSDDLSNNQLEAATGRGPSITRQGRD
                     FRTWLASELDRVAGDKGKAKEDLSPKEVKDVWQNAKPPRKDIQVRIGLGFFTSVPLLY
                     STVRHEFIHVEQLRNNYLGILLDQVLPPRVKVPDIGGKVADFEIDPYLWEMEHLANTG
                     LKDPADLNSLLVSGDEKLGEASKTVQSANRGRFETAYQAVWKKSMDETIAGITDEHTK
                     FTTDGKVGNAARVSKLDESLGLLWKNKDKHKNKPDGYAAGHKTATAQIAELVAASKAE
                     AFGKLLDTIDTEVASGYRRGLTAYERWQEVTAAWNGLAPKAKAAIKARYDTAAPALWT
                     KVFEVLEAEIQDYVSRGDVRAAEETMTNSVAPLFKNAYATVKTENFEARRKALKAAIA
                     EAKKAKPK"
     gene            1282..1743
                     /locus_tag="VF516_21835"
     CDS             1282..1743
                     /locus_tag="VF516_21835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110395.1"
                     /translation="MAASDDDAPPPTPQTLELTLAAGDAEGRVTLRIAQRGHAPVTVV
                     VPDRADQVEWFSPDSARLADPFLRVMWTAWRTDDGNAMHAGHQAALVFTRHVTLGPDD
                     VARATLDLKPALDGVLLRDGERTAWCTRAWIVGGVHPLPSNIVCWPDPSRE"
     gene            1988..2896
                     /locus_tag="VF516_21840"
     CDS             1988..2896
                     /locus_tag="VF516_21840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110396.1"
                     /translation="MIRSLSLQILSMSSALLVSACMAAPTSVDQRNASTIEDPAGDDG
                     AAPAGGTVVRMLALHPVASATAGAEVAAACTRVVYEGGPLLTHVDVHPVFWNSAVRLQ
                     SNLNAFYSAVVTSPLFAVLSQYMIGFGTAHAGIVDSRTTANVTDATVQSELNRLITAG
                     LLPAPTANTYYPVHFPSGMTITAPDGSKSCVQFCAYHSTFVRSGVNVNYGIFPDLGSA
                     GCAGGCGTSTLTNNTDDVASNELVDAVTDPGAGLATSCILPPVGWIVPTTCQELGSLC
                     NGQQGTIPGFAVEKVWSNQACACVDH"
     gene            complement(2931..4730)
                     /locus_tag="VF516_21845"
     CDS             complement(2931..4730)
                     /locus_tag="VF516_21845"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF009560.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HEX8110397.1"
                     /translation="MTAPKDKRALRSRLWFDDASNPGMTALYLERYLNFGLTRGELQS
                     GKPIIGIAQSGSDLSPCNRHHLELALRVREGIREAGGVAIEFPTHPIQETGKRPTAAL
                     DRNLAYLGLVEVLYGYPLDGVVLTTGCDKTTPAFLMAAATVNLPSIVLSGGPMLNGWF
                     QGERTGSGTIVWRSRERLAAGEIDYQQFMELVASSAPSAGHCNTMGTASTMNSLAEAL
                     GMSLPGCAAIPAPHRERAQIAYETGKRIVGMVWEDLKPSDILTRKAFENAIVVNSAIG
                     GSTNAPIHLNALARHVGVELSMADWQTVGHRIPLLVNLQPAGAYLGEEYHRAGGVPAV
                     VRELMAHGKIHEDAGTVTGRTIGDNCRNAPVPDGDVIRRFDAPLVRDAGFIVLSGNLF
                     DSAIMKTSVISAEFRERYLCNPDDPMAFEGRAVVFDGPEDYHHRIDDPALQIDERSIL
                     VIRGTGPIGYPGGAEVVNMQPPAALIQRGIRSLPCIGDGRQSGTSGTPSILNASPEAA
                     AGGGLALLETGDGVRVDLGKGQANVLIAPEELERRRARLAASGGYRYPDSQTPWQELY
                     RQTVGQHATGACMDFATRYQDLIVRFGPPRHSH"
     gene            complement(4768..>5618)
                     /locus_tag="VF516_21850"
     CDS             complement(4768..>5618)
                     /locus_tag="VF516_21850"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8110398.1"
                     /translation="AATGKIEHFELPGTLRIRYHARARDVASENVIGRIDGADPQLAR
                     EAVVVSAHLDHLGVDASGAIRNGAIDNASGIAELLLVARALAAQPRPPRRTVILLATT
                     GEELGLLGAEAFTAHPTWPIDRIAANINIDQTGMMWPIVDVVADGAERSTLAAPVARA
                     TAALGLAVSPDPVPEQGFFLRSDQAPFARAGVPSLIFRPGFRDASGGTDRNRALFTRW
                     RSTAYHSPLDKIDQRDPPIDFASGAAMAHLVYLTALEVANADARPSWNPGDVFAPGPA
                     AMAHSR"
BASE COUNT          851 a         2044 c         1920 g          803 t
ORIGIN      
        1 caaggcgctc gcgctgctcc tcaccgcgct gcaggggctc aacgcgacgc tgttcaagtc
       61 cgatgacctc agcaacaacc agctcgaggc cgcgacgggc cgcggcccca gcatcacccg
      121 ccagggccgc gacttccgga cgtggctcgc gtccgagctc gaccgcgtcg ccggtgacaa
      181 gggcaaggcc aaggaggacc tgtcaccgaa ggaggtcaag gacgtgtggc agaacgccaa
      241 gccgccgcgc aaggacatcc aggtccggat cggcctcggg ttcttcacca gcgtcccgct
      301 gctgtactcg accgtccgcc acgagttcat ccacgtcgag cagctgcgca acaactacct
      361 cggcatcctg ctcgaccagg tgctgccgcc gcgggtgaag gtgcccgaca tcggcggcaa
      421 ggtcgccgac ttcgagatcg atccgtacct gtgggagatg gagcacctcg cgaacaccgg
      481 cctgaaggat ccggccgatc tgaacagcct gctggtctcc ggcgacgaga agctcggcga
      541 ggcgagcaag acggtccagt cggcgaatcg cggccggttc gagaccgcct accaggcggt
      601 gtggaagaag tcgatggacg agaccatcgc cgggatcacc gatgagcaca cgaagttcac
      661 caccgacggc aaggtcggca acgccgcgcg cgtgagcaag ctcgacgaga gcctgggcct
      721 cttgtggaag aacaaggaca agcacaagaa caagcccgac ggctacgcgg ccggccacaa
      781 gaccgccacg gcgcagatcg ccgagctcgt cgccgcgagc aaggccgagg cgttcggcaa
      841 gctgctcgac accatcgaca ccgaggtcgc gagcggatac cggcgaggac tcaccgcgta
      901 cgagcgctgg caagaggtca ccgcggcgtg gaacggcctc gcccccaagg cgaaggcggc
      961 gatcaaggcg cgctacgaca ccgccgcgcc ggccctgtgg accaaggtgt tcgaggtgct
     1021 cgaggccgag atccaggact acgtctcgcg cggcgacgtc cgcgccgccg aggagaccat
     1081 gaccaatagc gtcgccccgc tgttcaaaaa cgcctacgcg acggtcaaga ccgagaactt
     1141 cgaagctcgc cgcaaggccc tgaaggccgc gatcgccgag gccaagaagg ccaagccgaa
     1201 gtgaacccct ggcgcgccgc cccgcttctg ctcgcgctcg ccgcctgcgc gggcccggcg
     1261 cgcccagccc caggtactcc catggcagct tccgacgacg acgcccctcc ccccacgccc
     1321 cagaccctcg agctcacgct tgctgccggc gacgccgagg gtcgcgtcac cctgcgcatc
     1381 gcccagcgcg gccacgcccc ggtcacggtg gtcgtccccg atcgcgccga tcaggtcgag
     1441 tggttctccc ccgacagcgc ccgcctggct gatccattcc tgcgcgtcat gtggaccgcc
     1501 tggcgcaccg acgacggcaa cgccatgcat gccggccacc aggccgcgct cgtgttcacc
     1561 cgccacgtca cgctcggccc cgacgacgtc gcccgcgcca cgctcgatct caagcccgcg
     1621 ctcgatggcg tgctcctgcg cgacggcgag cggaccgcct ggtgcacgcg cgcctggatc
     1681 gtcggcggcg tgcacccgct gccgtccaac atcgtgtgct ggccggaccc atcgcgcgag
     1741 tagtcgcaag cgcggctagc gacggcttcc ggttcgacgc gctcggcaac ctgtggacca
     1801 gcgccggcga cggcgtccac tgctacgccc cgacagcacg cggctcggca agatccgatt
     1861 gttcgtccgg gtcgtcgatg cagcaaaagg cgacaccgct cggccgccgg acggcgcgcc
     1921 ggccgtgctg acaatcgctc ggtcgtcgcg atccgcggcc gatcttcctg ctctccgggg
     1981 gggctccatg atccgttctc tctcgctcca gatcctgtcg atgtcgtcgg cgctgctcgt
     2041 ctcggcctgc atggcagcgc cgaccagcgt cgaccagcgc aacgccagca ccatcgaaga
     2101 cccagccggc gatgacgggg ctgcgccggc cggcggcacg gtcgtgcgca tgctggcgtt
     2161 gcatcccgtg gccagcgcga cggccggggc cgaggtcgcc gccgcgtgca cccgcgtggt
     2221 gtatgagggc ggccccctgc tcacgcacgt cgacgttcac ccggtgttct ggaactcggc
     2281 cgtgagattg cagtccaacc tcaatgcgtt ctactccgcc gtcgtcacga gtccgctgtt
     2341 cgccgtgctc agtcagtaca tgatcggatt cggcaccgcc cacgccggga tcgtcgactc
     2401 gcgcaccacg gcgaacgtga cggacgccac ggttcaatcc gagctgaacc ggctgatcac
     2461 ggcgggcctt ctgccggcgc cgaccgccaa cacctattat ccggttcact tcccttccgg
     2521 gatgaccatc accgcgccgg acggcagcaa gtcgtgcgtc cagttctgcg cctaccacag
     2581 cacgttcgtg cgcagcggcg tcaacgtcaa ctacggcatc ttcccggacc tcggcagcgc
     2641 aggctgcgcc ggtggctgcg gtaccagcac gctcacgaac aacaccgacg atgtcgcgtc
     2701 gaacgagctc gtcgacgccg tgaccgaccc cggcgccggg ctcgccacgt cgtgcatctt
     2761 accaccggtg ggatggatcg ttccgaccac gtgccaggag ctcggttcgc tctgcaacgg
     2821 gcagcagggc acgatccccg gattcgccgt ggagaaggta tggtcgaatc aggcgtgcgc
     2881 ctgcgtcgat cactgaacag gcacccggca ggggcagcac gggggactcc tcagtgcgag
     2941 tgccgcggcg gcccgaaccg cacgatgagg tcctggtagc gcgtggcgaa gtccatgcac
     3001 gccccggtcg cgtgctgtcc gaccgtctgg cgatacagct cctgccacgg cgtctggctg
     3061 tccgggtagc gatatccgcc gctcgccgcc aggcgcgccc gccgccgctc gagctcctcg
     3121 ggcgcgatca gcacgttcgc ctggcccttg ccgaggtcga cccgcacccc gtcgccggtc
     3181 tcgagcagcg cgagcccgcc gcccgccgcg gcctcgggcg acgcgttgag gatcgacggc
     3241 gtgcccgacg tccccgactg ccggccgtcg ccgatgcacg gcagcgatcg gatcccgcgc
     3301 tggatcagcg ccgccggcgg ctgcatgttc acgacctcgg cgccgcccgg atagccgatc
     3361 ggcccggtac cgcggatcac caggatcgac cgctcgtcga tctgcagcgc cggatcgtcg
     3421 atccggtggt ggtagtcctc ggggccatcg aacaccaccg cgcggccctc gaacgccatc
     3481 ggatcgtcgg ggttgcacag gtagcgctcg cggaactcgg ccgagatcac gctggtcttc
     3541 atgatcgccg agtcgaacag gttgccggac agcacgatga accccgcgtc gcggaccagc
     3601 ggggcgtcga accggcggat cacgtcgccg tcgggcaccg gggcgttccg gcagttgtcg
     3661 ccgatcgtcc gcccggtcac cgtccccgcg tcctcgtgga tcttgccgtg ggccatcagc
     3721 tcgcgcacca ccgcggggac cccaccggcg cggtggtact cctcgccgag gtacgccccc
     3781 gccggctgca ggttgaccag cagcgggatc cggtggccga ccgtctgcca gtccgccatc
     3841 gacagctcga cgccgacgtg gcgcgccagc gcgttgaggt ggatcggcgc gttggtcgag
     3901 ccgccgatcg ccgagttcac gacgatcgcg ttctcgaacg ccttgcgggt caggatgtcc
     3961 gacggcttga ggtcctccca caccatcccc acgatccgct tgccggtctc gtaggcgatc
     4021 tgcgcccgct cgcggtgcgg cgccgggatc gccgcgcagc ccggcagcga catgccgagc
     4081 gcctcggcga gcgagttcat cgtcgacgcc gtccccatcg tgttgcagtg cccggccgac
     4141 ggcgccgacg acgcgaccag ctccatgaac tgctggtagt cgatctcgcc ggcggcgagc
     4201 cgctcgcgcg atcgccacac gatcgtcccc gagccggtcc gctcgccctg gaaccagccg
     4261 ttgagcatcg gcccgcccga cagcacgatc gacggcaggt tgacggtcgc cgccgccatc
     4321 aggaacgccg gcgtggtctt gtcgcagccg gtggtcagca cgaccccgtc gagcggatag
     4381 ccgtacagca cctcgaccag cccgaggtag gccaggttgc gatcgagcgc cgcggtcggc
     4441 cgcttgccgg tctcctggat cgggtgggtc ggaaactcga tcgcgacccc gccggcctcg
     4501 cggatgccct cgcgcacgcg gagcgccagc tcgaggtggt ggcggttgca cggcgacagg
     4561 tcgctgcccg actgcgcgat gccgatgatc ggctttccgg actgcagctc gccgcgggtc
     4621 agcccgaagt tcaggtagcg ctcgaggtac agcgcggtca tccccgggtt gctggcgtcg
     4681 tcgaaccaca gccgcgagcg cagcgcccgc ttgtccttcg gtgcggtcat gtatcctccc
     4741 gcggggcgaa ccgcctcggc gtatcggtca gcgcgagtgt gccatggcgg ccgggccggg
     4801 tgcgaacacg tcgccggggt tccagctggg ccgcgcgtcg gcgttcgcga cctcgagcgc
     4861 cgtcaggtag acgagatgcg ccatcgcggc gccggaggcg aagtcgatcg gcgggtcgcg
     4921 ctggtcgatc ttgtcgagcg gcgagtggta cgccgtgctg cgccaccggg tgaacagggc
     4981 gcggttgcga tcggtaccgc cgctcgcgtc gcggaacccg gggcggaaga tcagcgaggg
     5041 tacgccggcg cgcgcgaacg gcgcctggtc gctgcgcagg aagaagccct gctcggggac
     5101 cggatcggga gacaccgcga gcccgagcgc ggcggtcgcg cgcgccacgg gggcggccag
     5161 cgtgctgcgc tcggcgccgt cggcgacgac gtcgacgatc ggccacatca tgccggtctg
     5221 atcgatgttg atgttcgcgg cgatccggtc gatcggccac gtcgggtgcg cggtgaatgc
     5281 ctcggcgccg agcaggccga gctcctcgcc ggtggtcgcc agcaggatca ccgtgcggcg
     5341 cggcggccgg ggctgcgcgg ccagcgcgcg ggcgacgagc aggagctcgg cgatgcccga
     5401 ggcgttgtcg atcgcgccgt tgcggatcgc gccgctggcg tcgacgccga ggtgatcgag
     5461 gtgggcgctg accacgaccg cctcgcgcgc cagctggggg tcggcgccgt cgatccggcc
     5521 gatcacgttc tccgatgcga cgtcgcgcgc gcgggcgtgg tagcggatcc gcagcgtgcc
     5581 gggcagctcg aagtgctcga tcttgccggt ggctgccc
//
LOCUS       DASVOR010001144         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_479241,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001144 DASVOR010000000
VERSION     DASVOR010001144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479241"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            251..604
                     /locus_tag="VF516_21855"
     CDS             251..604
                     /locus_tag="VF516_21855"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8110399.1"
                     /translation="MRRQYTAEQRREFLDLVTSGRATISEAAARLGVPPSTAYYWARK
                     AAAQPRPTRKATRALVPAKPPSASPPPFVQLIRASDTGAVIEVRIGHTAIQVRPGFDA
                     ELLRAVIELLREIVG"
     gene            628..942
                     /gene="tnpB"
                     /locus_tag="VF516_21860"
     CDS             628..942
                     /gene="tnpB"
                     /locus_tag="VF516_21860"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX8110400.1"
                     /translation="MALEPVDMRLGAERLGALIRERMRAEPRSRALFVFVGKRGHSMK
                     VLTWDGSGTIVVHKKLDAGKFELPRATTPGEQHLVVSDAIFEVIHKGVAVTPRGVRRR
                     VH"
     gene            1080..2708
                     /locus_tag="VF516_21865"
     CDS             1080..2708
                     /locus_tag="VF516_21865"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX8110401.1"
                     /translation="MAIKTETAVKVASLEDTIANLAHENTLLKRRLYGNRTEHSHTSE
                     LQLALGDLLTGEAELQKALNDAVGKVQDALEPEPKPAGPRPTPKGRRDLSLSKLPRVV
                     VEIVHEELEASGCRRIGFEDSSQLMFRRGGFAVLVKRVAKYEVIRDGEATVETVPSPE
                     TLFPRALLHSSAIAHVITSKFGMGTPHYRLEQDLADQDARLDRGTMCRYVEHAGNVFG
                     ATIVHAMWQDALTNAQVISTDATGALIQPAKTKDGRPQACKKGHFFTAVVDCDAILFV
                     YTEAHTSAFVQGLFGSFRGYLQADASNVYDVLEQGPPKDSEDGVRLVGCFAHLRRYFF
                     EAAICRYPVGLQGLFRIRAIYAADRVVQRAPATERTALRDQHVRPLVDDFFAWVHATR
                     PITIGRNLATKALGYAANQEAELRRILERGELPLDNTRAERALRKIVVGRKAWMFYGS
                     DTHAEAAAAIFSVIASCRLHGLDPFQYIDEILRVLPYWQRDRYLELSPKYWRATRGRL
                     RADELATPISAFEVPPPLGAAAAMSAPASAGPSP"
BASE COUNT          507 a         1051 c         1066 g          486 t
ORIGIN      
        1 gtcggcggct ccggccttgc gctcaccggc gccggtctct ggctcgttta ccgcagtcga
       61 aggggcacga cagccgcttc ggcggcgaca gccggcggga gcgggacggc tgtccgggtg
      121 ttgccagtca tctctctcga ctcttctcac gccctcatcg aatgggccgg gacgttttga
      181 tcaaatggca agtaaccgct gcgtcgatcg cacatggtag cccgggggac ttggtcgcga
      241 ccatgcgcgg atgcgcagac agtatacagc tgaacagcga cgagaatttc tcgacctggt
      301 gacttcgggg cgggccacga tctccgaagc cgccgcacga ttgggcgtgc ccccgtcgac
      361 ggcctactac tgggcccgga aggctgccgc gcagccccgt ccgacccgca aggccacgcg
      421 ggcgctcgta cccgccaagc ctccgtcggc ctccccgccg ccgttcgtgc aactcatccg
      481 cgcgagcgat accggcgcgg tgatcgaggt gcggatcggg catacggcga tccaggtacg
      541 gcctggcttt gatgcggagc tcctgcgagc ggtcatcgag ctcttgcgcg agatcgtcgg
      601 atgatcgcgc acgggttgcc gatctacgtg gcgctcgagc ccgtggacat gcgcttgggc
      661 gccgagcggc tcggggcgct gatccgcgag cggatgcggg ccgagccgcg ttctcgcgcg
      721 ctgtttgtgt tcgtcggcaa gcgcgggcac agcatgaagg tcctgacctg ggacggctcg
      781 ggcacgatcg tggttcacaa gaaactcgac gccgggaagt ttgagctacc gcgagcgacg
      841 acgccgggcg agcaacacct tgtcgtgagc gacgcgatct tcgaggtgat ccacaagggc
      901 gttgcggtca caccgcgcgg tgtacgacga cgcgttcact gagcggacgt tccggattgc
      961 gcggtcgatc cgcggcgcgt atagctgacc tcgtgtcgag cccaccgccg gatcccgcgc
     1021 cgagccccgt ggctggcgtc gatcttgcgg gccaactcgc agcgctgcag gcggagttgg
     1081 tggcgatcaa gaccgagacg gcggtcaagg tggcctcgct cgaggacacg atcgccaacc
     1141 tcgcgcacga gaacacgctg ctcaagcgcc ggctgtacgg caaccggacc gagcacagcc
     1201 acaccagcga gctgcagctc gcgctcggtg acctgctcac gggcgaggcg gagctgcaga
     1261 aggcgctcaa cgacgcggtc ggcaaggtcc aggacgcgct cgagccagag cccaagccgg
     1321 caggtccgcg gcccacccca aagggccggc gggatctgag cttgagcaag ctgccgcgcg
     1381 tggtcgtcga gatcgtccac gaggagctcg aggcgagtgg ctgccggcgg atcggcttcg
     1441 aggacagctc gcagctgatg ttccggcgcg gcggcttcgc cgtcctggtc aagcgcgtcg
     1501 ccaagtacga ggtcattcgc gacggcgagg cgacggtaga gaccgtgccg agccccgaga
     1561 cgctgtttcc gcgcgcgctg ctgcacagct cggcgatcgc gcacgtcatc acctcgaagt
     1621 tcgggatggg cacgccgcac tatcgtctcg agcaagatct cgccgaccag gacgcgcggc
     1681 tcgatcgggg cacgatgtgt cgctacgtcg agcacgccgg caatgtgttc ggcgcgacga
     1741 tcgtccacgc gatgtggcaa gacgcgctga ccaacgcgca ggtgatctcg accgacgcca
     1801 ctggcgcgct gatccagccg gcgaagacca aggatggtcg tccacaggcg tgcaagaagg
     1861 ggcacttctt taccgccgtc gtcgactgcg acgcgatcct gttcgtgtac accgaggcgc
     1921 acaccagtgc gttcgtgcag ggcctgttcg gcagcttccg cggctacctg caggcggacg
     1981 cgagcaacgt gtacgacgtc ctcgagcagg gtccgccgaa ggacagcgag gacggcgtgc
     2041 ggctcgtcgg atgtttcgcg catctgcgca gatacttctt cgaggcggcg atctgccgct
     2101 accctgtcgg gctgcagggc ttgttccgaa tccgggcgat ctacgccgct gatcgcgtgg
     2161 tgcagcgcgc gcccgcgacc gagcgcacgg cgctccgcga tcaacacgtg cgaccgctcg
     2221 tcgacgactt ctttgcgtgg gtccacgcga ctcggccgat caccatcggc cgcaacctcg
     2281 cgaccaaggc gctcggctac gcggccaacc aggaagctga actccggcgc atcctcgaac
     2341 gcggcgagct gccgctggac aacacacggg ccgaaagagc gctccggaaa attgtcgtcg
     2401 ggcgcaaggc gtggatgttc tacggcagcg atacgcacgc cgaggctgcc gctgcgatct
     2461 tcagcgtcat cgcctcgtgc cggctgcacg gcctcgaccc attccagtac atcgacgaga
     2521 tcctgcgtgt cttgccctac tggcagcgag accgttacct cgagctctcg cccaagtact
     2581 ggcgcgccac gcgcggccgg ctccgagccg acgagctcgc gactccgatc tctgccttcg
     2641 aggtgccgcc accgctcggc gcggccgcgg cgatgtctgc gcctgccagc gccggcccga
     2701 gtccatagcc gatccgccgg agcgaaggct atcggtgctc gtgctccgcg tgccgcgcgc
     2761 cgtccgcgcg atcgccggag tcggcgaacg ccagcgccgg gccggccgcc caccgcggca
     2821 acctgacttg gaggcggcag gctctggcac gttggcatct tcgctgcggg cagggtcgac
     2881 ctcaatgtgg ggtcggcgca ccggatacgg tgtcgatctc gacggtcaag agcgttcttg
     2941 gcgccgatgg caccgcggac gctggaaatc agatcggcac gatcttgccg aagcacaccg
     3001 gagcggcagg cgcatcgccg gcgcttggag catcggggtt cacgtcaggc ggacgcggga
     3061 acccgcatcc catcatggcg acggacacga ccaggcacgc cttcatgcgg
//
LOCUS       DASVOR010001145         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_303889,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001145 DASVOR010000000
VERSION     DASVOR010001145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303889"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..715
                     /locus_tag="VF516_21870"
     CDS             257..715
                     /locus_tag="VF516_21870"
                     /inference="COORDINATES: protein motif:HMM:NF022182.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HEX8110402.1"
                     /translation="MTALFANHRETNLISTVALVEQVLGDLGYPPADHRVSEPGASHV
                     WRLVTGSAITRVALVERTEFPHLRITAVVMTLDAAVDRAALFGHLLELNAKLCGVAFA
                     TDGDHVLLVSERSTVDLDDSEVRDAIERVTTYADEHDDALVARFGGRVGA"
     gene            complement(729..986)
                     /locus_tag="VF516_21875"
     CDS             complement(729..986)
                     /locus_tag="VF516_21875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110403.1"
                     /translation="MPNYAKLHDLISHRVSIEYDTGARVTGYLASCQPSSGPVEFVVL
                     SDAKLIDSQGRVLRETGELTMCPNLLTSIALDEGPRGRDIK"
     gene            complement(1002..1355)
                     /locus_tag="VF516_21880"
     CDS             complement(1002..1355)
                     /locus_tag="VF516_21880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4333 domain-containing protein"
                     /protein_id="HEX8110404.1"
                     /translation="MRTLAALAFIAGVAAVAIGLARTPTVADGRVLAADLREKVRKDG
                     ANVTEMECDREIPIGVRGATFTCIATLPDGETQVVDWALTPDGQYEARPQPPTRGPRP
                     RLRKPAPGDPWAERP"
     gene            1393..>1767
                     /locus_tag="VF516_21885"
     CDS             1393..>1767
                     /locus_tag="VF516_21885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8110405.1"
                     /translation="MPRDPDGKRPPKLRKTRFATDADIEIQGTYGPASAPAEPSSPPG
                     QFPFTRGVQETMYRGQLWTMRQYAGFGTAAESNRRYKYLLAQGGKGLSVAFDLPTQMG
                     RDSDHPMARGEVGRVGVAIDSLE"
BASE COUNT          250 a          686 c          585 g          246 t
ORIGIN      
        1 cgccgggtcc ggatcgccgg gagaatccgc aggcggaggc gacgtgcgat ccggcaacgg
       61 atcaccggcc ccagcaggcg gcggcccgtc cgcgatcgat ccatccggct cgtctggcac
      121 cccatccacc atagcgcgcc ccgcgctgcg ccacggccgc ctcgtctacg atgtcagatc
      181 acaatgtgcg cgcgccaccc gcgcgccgcg gcgccgagcg atgccggtcc gagtcgacct
      241 gaggtaccct gcgaccgtga ccgcgctgtt cgccaaccat cgcgagacca acctgatctc
      301 cacggtggcc ctcgtcgagc aggtcctcgg cgatctcggc tatccgccgg cggaccaccg
      361 ggtcagcgag cccggcgcct cgcatgtctg gcgcctggtc acgggctcgg cgatcacccg
      421 cgtcgccctg gtggagcgca cggagttccc gcacctccgg atcacggccg tggtgatgac
      481 gctcgacgcc gcggtcgacc gcgccgcgct gttcggccac ctcctggagc tcaacgcgaa
      541 gctgtgcggc gtcgcgttcg ccaccgacgg cgaccacgtg ctgctggtca gcgagcgatc
      601 gaccgtcgat ctcgacgaca gcgaggtccg cgacgcgatc gagcgcgtga cgacctacgc
      661 cgacgagcac gacgacgcgc tggtcgcgcg gttcggcggc cgggtcggcg cctgacgctc
      721 gcccggcgct acttgatgtc gcgcccgcgc ggcccctcgt cgagcgcgat cgaggtcagc
      781 aggttggggc acatcgtcag ctcgccggtc tcgcgcagca cgcggccctg cgagtcgatc
      841 aacttggcgt ccgacagcac gacgaactcg accggccccg agctcggctg acacgacgcc
      901 aggtacccgg tgacgcgcgc gccggtgtcg tactcgatgg agacgcgatg gctgatgaga
      961 tcgtgcaact tcgcgtagtt cggcattccg ggctcgctat atcacggcct ctccgcccat
     1021 ggatcgcccg gcgccggctt ccgcagccgc ggccgcggcc cgcgggttgg cggctgcggc
     1081 ctggcctcgt actggccgtc cggtgtcagc gcccagtcga ccacctgggt ctcaccgtct
     1141 ggcagggtcg cgatgcaggt gaacgtcgcg ccccggaccc cgatcgggat ctcccggtcg
     1201 cattccatct cggtcacgtt cgccccgtcc ttgcgaacct tctcccgcag atccgccgcg
     1261 agcacccggc cgtcggccac cgtcggcgtg cgcgccagac cgatcgccac cgccgcgacc
     1321 ccggcgatga acgcgagcgc cgccagcgtt ctcatcgcgc cgaggatagc ggatctgcgc
     1381 tacacccgga acgtgccccg agatccagac ggcaagcgcc cccccaagct ccgcaagacc
     1441 cggttcgcca ccgacgccga catcgagatc cagggcacct acgggccggc cagcgcgccg
     1501 gccgagccgt ccagcccgcc cggccagttc cccttcaccc gcggcgtgca ggagaccatg
     1561 taccgcggcc agctgtggac gatgcgccag tacgccgggt tcggcaccgc cgcggagtcc
     1621 aaccgccgct acaagtacct gctcgcccag ggcggcaagg gcctgtcggt cgcgttcgac
     1681 ctgccgaccc agatgggccg cgactccgat cacccgatgg cgcgcggcga ggtcggccgg
     1741 gtcggcgtcg cgatcgactc gctcgag
//
LOCUS       DASVOR010001146         7666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_273895,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001146 DASVOR010000000
VERSION     DASVOR010001146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7666
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273895"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1539
                     /locus_tag="VF516_21890"
     CDS             <1..1539
                     /locus_tag="VF516_21890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110406.1"
                     /translation="PPPSGAPAPGGPSGSPAPDPYEEFKRAINASDAATAKAKWALVT
                     TKNKLATESHDFKWTMAYVLHEDAAVMIKEGGLDITEFIYRIFNDPRFDQWLGALRTN
                     NLLTGFLNAEPKKGVVSADMAQKLKGWANAATSAPEATAIFEKVYPHLNTTAFSGEVA
                     APWTQPTIARLYGILATQLPVGHVQTVTGGFTLMRQHTQDGKLVTTNFAWWNPDIFRV
                     VLNESSSGANEGGTTHDMTGGSRSGASSAYTKPDGSAGTQTTQTHFQGSALHEVGHGV
                     GARMGGDSYAENAGNYPGWTNVGRGSWISGMWTGGAGPNGTKPTGLHSDADLSAGDAK
                     DFLATEIAEGAGKYNAGWFNDAPRADIARYVNWKYGNEPLGKYWNAVVNGGLGADNAY
                     SVSDPSRVRGDWTYGYLTRFHGSPWAKFKTAAYNNKVSWYGISSPKEWWAEQYAHYYR
                     TEKTGGGLIDPATKKLLDDLDKKQFLPTNASGTQGISFGSDAQVAMAQPGSALRREPA
                     MFPW"
     gene            1583..2233
                     /locus_tag="VF516_21895"
     CDS             1583..2233
                     /locus_tag="VF516_21895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110407.1"
                     /translation="MTARWTLAVPAAIAAALVALDAGCPKREPSADTPPRPGSSADSP
                     RPGPGDTLTTTGNPWDQARAIAEKTGASSLSKWKDDVPYLFQAKGSRGVLVHKGAVVT
                     ARGPAAAGAYLRDIGILDGNGPSPDSLLTLFYVLHAFPTVEGLPEQSAVDTLGPPDLQ
                     PRVERAGGHARVILSYRLPPDPGPSRGTVPVMRETLDIGPSGAAAWTGERIDWKLP"
     gene            2268..2960
                     /locus_tag="VF516_21900"
     CDS             2268..2960
                     /locus_tag="VF516_21900"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HEX8110408.1"
                     /translation="MSITAVREVVCRCGAPVRVFVATSVNAERHPHMREAILRRTFHV
                     FECGACHQPVGIDKEFLYIDLARREFYGVFPLAAAADPEPCARKIAEAFDTALGAGAG
                     EAVRALAEGMRVRVCFGLEELREKIAARAAGLADLALEVLKADILAANPDLAQLGIQT
                     LRLEAVGEDGNLGLLLEQAGDPPRLLQDRAFTVERAAYDAFDVLPWEDLIARLPGIAS
                     GPHVSLLRLARA"
     gene            3675..5024
                     /locus_tag="VF516_21905"
     CDS             3675..5024
                     /locus_tag="VF516_21905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110409.1"
                     /translation="MTLVRFGSRTAGGIACAIALAALTPHAALAGDLRYRSFSASAAI
                     GPPAEAYAAKLQTVTRTAMGAAGEVHLVKLPGIPAIPAPFAGDIVSAVAAGEAGGGFD
                     AAYISGGDLNRAWGFLFNSGVPFGPTFDEFLGFLYGKSIDSGQKTGLELVQELLDLRN
                     RSVIAVPIVGSPEQLSGYFFEPMESVRGHQGIGLAGLCEQPWRLRYLPPGENVLNQAC
                     DDLVASHRIARKNISFVEAVPGGGSLIDAVLAGTLDGFEFATALDDVSQLFNTANNPG
                     TVGLRFVHTPGWQQQFLITWMLINKQVWSSLSAGQQILIQTVARDHVLSSYGENLRQQ
                     GSALQFILDANKHDGNPDDNLVLSRWPTHDLARLRDATIRVLNGRVTDPAFAATDRAD
                     YARVLEALRLYVRANDHYWNLREVPTSMRFEDWANPAGACWEAATCEPEHTRPGHRW"
     gene            5139..5525
                     /locus_tag="VF516_21910"
     CDS             5139..5525
                     /locus_tag="VF516_21910"
                     /inference="COORDINATES: protein motif:HMM:NF041086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAUR_1299 family protein"
                     /protein_id="HEX8110410.1"
                     /translation="MSIDLRPVLEKAFLVEEVENASEVVARAEQRFEEVTRAGARVFE
                     IVAPGDPDEEWLREQLVHRLVYVCESEGVPVPECSGTIVALFIGPRLYAIPAEDVIRW
                     ASRILNTSVEQLRMQYGTHEVETALR"
     gene            5733..6488
                     /gene="modA"
                     /locus_tag="VF516_21915"
     CDS             5733..6488
                     /gene="modA"
                     /locus_tag="VF516_21915"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8110411.1"
                     /translation="MKKLLIAAAIVLFGSAVTTRAEDSRELTVFAAASLRESFEDLAK
                     TFEARSPGVKVRLNLAGSQELRTQIENGARADVFASADQKRMAALVKANLVGTPRVFA
                     RNTPVVIVPRGNPSKIGSFDQLPRAKKIVIGVPEVPIGTYTLEILDKAGADFKQKVLA
                     NVVSREPNVRQVMAKVSLGEADAAIVYRTDAMAGKDKVEVIEIPAKVNVVAEYPVAVL
                     ANAPEAAAARAFVDLLLSADGQKRLGAAGFVAP"
     gene            6538..7275
                     /locus_tag="VF516_21920"
     CDS             6538..7275
                     /locus_tag="VF516_21920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8110412.1"
                     /translation="MLAFLVVPLVALFVTGTPADWLAGLRHPITAPALLLSLTTTALS
                     LGVLVVFGTPLAWWLGRSNARAARFVETAVQLPIVVPPAVAGLALLLAFGRRGLIGPL
                     LSGLGIDIAFSTIAVVIAQVFVAAPFYIQAATAAFAGIDENLLVVARTLGARPGAVLF
                     RVALPLARRGLIAGAALAWARALGEFGATLMFAGNLTGRTQTLPLAVYTALESDVRAA
                     QALAGILVVIALAIIVFLRPRRGRSRG"
     gene            7272..>7666
                     /locus_tag="VF516_21925"
     CDS             7272..>7666
                     /locus_tag="VF516_21925"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8110413.1"
                     /translation="MTALVANIGVRAGALEIEVALEVDDRGLVLVGPNGAGKTTVLLA
                     LVGARRPTRGRITIAGDVLFDGAEGVDRATEERGLAYLPQDYALFPHLTAAANVAFGL
                     HGPRAERMRKARALLDRLGVAAVADRHPGA"
BASE COUNT         1116 a         2841 c         2617 g         1092 t
ORIGIN      
        1 ccgccgccgt cgggcgcacc ggcgcccggc ggaccctcgg gcagcccggc cccggatccc
       61 tacgaggagt tcaagcgggc gatcaacgcc agcgatgcag cgaccgccaa ggccaagtgg
      121 gccctggtca ccaccaagaa caagctcgcg accgagagcc acgacttcaa gtggaccatg
      181 gcgtacgtcc ttcacgagga cgccgcggtg atgatcaagg agggcggcct cgacatcacc
      241 gagttcatct accggatctt caacgacccc cggttcgatc agtggctcgg cgcgctgcgc
      301 accaacaacc tgctcaccgg cttcctcaac gccgagccca agaagggcgt cgtcagcgcc
      361 gacatggccc agaagctcaa gggctgggcc aacgccgcga ccagcgcgcc cgaggccacc
      421 gcgatcttcg agaaggtcta cccgcacctc aacacgaccg cgttctccgg cgaggtcgcg
      481 gcgccgtgga cccagccgac gatcgcccgg ctgtacggca tcctcgcgac ccagctgccg
      541 gtcggccacg tccagaccgt gaccggcggg ttcacgctga tgcgccagca cacccaggac
      601 ggcaagctgg tcaccaccaa cttcgcgtgg tggaaccccg acatcttccg cgtcgtgctc
      661 aacgagtcgt cgtcgggcgc gaacgagggc ggtaccaccc acgacatgac cggcggcagc
      721 cggtccggcg cgagctcggc ctacaccaag cccgacggct cggccggcac gcagaccacc
      781 cagacccact tccagggctc ggcgctccac gaggtcggcc acggcgtcgg cgcgcggatg
      841 ggcggcgaca gctacgccga gaacgcgggc aactaccccg ggtggaccaa cgttggccgc
      901 ggctcctgga tcagcgggat gtggaccggc ggcgcgggcc ccaacggcac caagccgacc
      961 ggcctgcaca gcgacgccga tctgtccgcg ggcgacgcca aggacttcct cgccaccgag
     1021 atcgccgagg gcgccggcaa gtacaacgcc ggctggttca acgacgcgcc gcgcgccgac
     1081 atcgcgcgct acgtcaactg gaagtacggc aacgagccgc tcggcaagta ctggaacgcg
     1141 gtggtcaacg gcgggctcgg cgccgacaat gcctattccg tatccgaccc atcacgggtc
     1201 cgcggcgact ggacctacgg ctacctgacc cggttccatg gctcgccgtg ggccaagttc
     1261 aagaccgccg cctacaacaa caaggtgtcg tggtacggca tctcgtcgcc caaggagtgg
     1321 tgggccgagc agtacgccca ctactaccgc accgagaaga cgggcggcgg cctgatcgat
     1381 ccggcgacca agaagctgct cgacgatctc gacaagaagc agttcctgcc gaccaacgcg
     1441 tcgggcaccc agggcatctc gttcggcagc gacgcccagg tcgcgatggc gcagccgggc
     1501 agcgcgctgc gccgcgagcc ggcgatgttc ccgtggtgac cggcgcggcg ctccccaccc
     1561 ggcgtgctag catcgccgca gcatgacagc tcgctggacg ctggcagtcc cggccgcgat
     1621 cgccgccgcc ttggtcgccc tggacgccgg atgtccgaag cgcgagccgt ccgcggatac
     1681 gccgcccagg ccggggtcgt ccgcggactc gcccaggccc ggcccaggag acaccttgac
     1741 caccaccggc aacccctggg atcaggcgcg ggcgatcgcc gagaagaccg gcgcgtccag
     1801 cctgtcgaag tggaaggacg atgtcccgta tctgttccag gccaagggca gccgcggcgt
     1861 gctcgtgcac aagggcgcgg tcgtcaccgc gcgcgggccg gcggccgccg gggcgtacct
     1921 gcgcgacatc ggcatcctcg acggcaacgg tccgagcccc gatagcctgc tcaccctgtt
     1981 ctacgtcctg cacgcgttcc ccaccgtcga gggcctgccc gagcagagcg cggtcgacac
     2041 gctcggcccg cccgacctcc agccccgcgt cgagcgcgcg ggcggccacg ccagggtgat
     2101 cctcagctat cgcttgcccc cggaccccgg cccgtcgcgc ggcaccgtgc cggtgatgcg
     2161 cgagacgctc gacatcggcc cgtccggcgc cgccgcctgg accggcgagc gcatcgactg
     2221 gaagctgccc tgagcccgcg cgcgcacacc cgccgggagg cggactgatg tcgatcaccg
     2281 ccgtgcgcga ggtcgtgtgc cggtgcggcg cgccggtccg cgtgttcgtc gcgaccagcg
     2341 tcaacgccga gcgccacccg cacatgcgcg aggcgatcct gcgccgcacg ttccacgtct
     2401 tcgagtgcgg cgcgtgccac cagccggtcg gcatcgacaa ggagttcctc tacatcgacc
     2461 tggcgcgccg cgagttctac ggcgtgttcc cgctcgccgc cgccgccgac cccgagccgt
     2521 gcgcgcgcaa gatcgccgag gcgttcgaca ccgcactggg cgccggcgcc ggcgaggcgg
     2581 tgcgggcgct cgccgagggc atgcgcgtcc gcgtgtgctt cggcctcgag gagctgcgcg
     2641 agaagatcgc cgcccgcgcc gccggcctgg ccgacctcgc gctcgaggtc ctcaaggccg
     2701 acatcctcgc ggccaacccc gatctcgccc agctcggcat ccagaccctg cgcctcgagg
     2761 cggtcggcga ggacggaaac ctcggcctgc tgctcgagca agccggcgat ccgccgcgcc
     2821 tgctccagga ccgcgcgttc accgtcgagc gtgccgccta cgacgcgttc gacgtcctgc
     2881 cgtgggagga cctgatcgcg cggctgcccg gcatcgcgtc cggcccgcac gtcagcctgc
     2941 tgcggctcgc gcgcgcctag ccgacgccta gcagatcagg tcggatcctg gtcgtacgtc
     3001 cggatgcctg ccggcagctg gacccacgaa tgtccagcgc gaggctgccg atctgcgcgc
     3061 aggcgtgccc ggtgatgcgc ccgcgcgcta cgcgcattcc ccgtattcgg tccgcgccgt
     3121 ccgaccgagc gaccctcgcg agggcgacgc cctcggccgt gcttcggccg atcgaatcca
     3181 tgtcgggtcc gatgcacttt cggagcaagc tggtgcggag ctcgtcggcg cgactccgag
     3241 ttcttcggat agcacgcgcc gcgaattcgt cgaccgtgtg tcgtgggcgc tcgcgctgcg
     3301 cggttctcgc gggccagctc agccggcccg accgcccgcg cacggcgttc cacctcggcg
     3361 gcgcgccact ccacgccacg tcgcccggta tgcaggtgag caccggtcga gagaaactcg
     3421 gatcgtccac ctggacgccg gaccgtccat gcaggagact ccggcgcact cgaatgcagg
     3481 cacggatcga gcggagtgcg ctcgcgtttg atctcgatgt gaaattcatt cctggagccg
     3541 cgccgctcgg ttagcgcgcc tgttcgctct cggcgcgatc cccgacggag atggtgctcc
     3601 gcggatcgcg catcggcgct gcgattgcac acgcgcgtcg agcacacgca tcgtacgagg
     3661 aaggaccgcc cgtcatgacc ctagttcgct tcggatcgcg taccgccggt gggatcgcgt
     3721 gcgcgatcgc gctggccgca ctgacacccc acgcggcgct cgccggcgat ctccgatatc
     3781 gcagcttcag cgcctcggcg gcgatcggcc cgccggccga ggcctacgcc gccaagctgc
     3841 agacggtcac gcggaccgcg atgggcgccg cgggagaggt ccacctcgtc aagctgccgg
     3901 gcatcccggc gatcccggcg ccgttcgccg gcgacatcgt ctcggcggtg gcggccggcg
     3961 aggccggcgg tggattcgac gccgcgtaca tctcgggcgg cgatctcaac cgcgcctggg
     4021 gcttcctctt caactcgggc gtgccgttcg gtccgacgtt cgacgagttc ctcggctttc
     4081 tctacggcaa gtcgatcgac agcggccaga agaccggcct ggagctcgtc caggagctgc
     4141 tcgatctgcg caaccgcagc gtcatcgccg tcccgatcgt cggtagcccg gagcagctgt
     4201 ccgggtactt cttcgagccg atggagtcgg tccgcggcca ccagggcatc gggctggccg
     4261 ggctgtgcga gcagccgtgg cgcctgcgct acctgccgcc cggcgagaac gtgctgaacc
     4321 aggcgtgcga cgacctcgtc gccagccacc ggatcgcgcg caagaacatc tcgttcgtcg
     4381 aggcggtccc gggcggcggc tcgctgatcg acgcggtcct ggccggcacg ctcgacggct
     4441 tcgagttcgc caccgcgctc gacgacgtct cccagctgtt caacaccgcc aacaacccgg
     4501 gcaccgtcgg cctgcgcttc gtccacacgc cgggctggca gcagcagttc ctgatcacct
     4561 ggatgctgat caacaagcag gtctggagct cgctgagcgc cgggcagcag atcctgatcc
     4621 agaccgtcgc gcgcgatcac gtgctgtcct cgtacggcga gaacctgcgc cagcaggggt
     4681 ccgcgctgca gttcatcctc gacgccaaca agcacgacgg caatcccgac gacaacctgg
     4741 tgctgtcccg gtggcccacc cacgacctgg cgcggctgcg cgacgcgacg atccgcgtgc
     4801 tcaacgggcg cgtcaccgac ccggcgttcg ccgccaccga tcgcgccgac tacgcccggg
     4861 tgctcgaggc gctccgcctc tacgtgcgcg ccaacgacca ctactggaat ctccgcgagg
     4921 tccccacgtc gatgcgcttc gaggactggg ccaacccggc cggcgcgtgc tgggaggcgg
     4981 cgacctgcga gcccgagcac acccgcccgg gccaccgctg gtagcgcggc cggtccgctc
     5041 ggcgggtcga gcaatgcctg cgcggtcctc gcagcgcggc ccaccgcagc gacgtggcat
     5101 gcccgtcgca cccgcagcag ggcagggagc aacacaccat gtcgatcgat ctgagacccg
     5161 tactcgagaa agcgttcctc gtggaagagg tggagaacgc ctccgaggtc gtcgcgcgcg
     5221 cggaacagag gttcgaggag gtcacccgcg ccggcgctcg ggtgttcgag atcgtcgcgc
     5281 ccggtgatcc cgacgaggag tggctgcgcg agcagctcgt ccatcgcctg gtctacgtct
     5341 gcgagtccga gggcgtgccc gtgccggagt gcagcggcac gatcgtcgcg ctgttcatcg
     5401 gcccgcggct ctacgccatt cctgccgagg acgtcatccg ctgggccagc cggatcctga
     5461 acacctcggt cgagcagctc cgcatgcagt acggcacgca cgaggtcgag accgcgctgc
     5521 gctgaggttc gagtctgccc gccggcctcg cgagccgccg ccgaggtgac acgcaccccg
     5581 gcacgatcgg cgtgcgctgc gtcgtgcctc acgttacatc tgcgcaactt cttgcaaatc
     5641 gccgggggct cgcattgctc gatggaggag cgctgctgta catcgtacct atccccacgc
     5701 gggccggaca tccccgcgag gcggggcccc ccatgaaaaa gcttctcatc gcagcagcga
     5761 tcgtgctgtt cgggtccgcg gtcacgaccc gggccgagga ctctcgcgag ctcaccgtgt
     5821 tcgcggcggc atcgctgcgc gagtcgttcg aggacctcgc caagaccttc gaggccaggt
     5881 cgcccggcgt gaaggtgcgc ctgaacctgg ccggcagcca ggagctccgc acgcagatcg
     5941 agaacggcgc gcgcgccgac gtgttcgcgt ccgccgatca gaagcgcatg gcggcgctgg
     6001 tcaaggcgaa cctggtcgga acgccgcgtg tgttcgcccg caacacgccg gtcgtcatcg
     6061 tgcccagggg caatccgtcg aagatcggct cgttcgatca gctgccccgg gccaagaaga
     6121 tcgtgatcgg cgtccccgag gttccgatcg ggacctacac gctggagatc ctcgacaagg
     6181 ccggcgccga cttcaagcag aaggtcctgg ccaacgtcgt gtctcgcgag cccaacgtac
     6241 gccaggtgat ggccaaggtg agcctgggcg aggccgacgc cgcgatcgtg taccggaccg
     6301 acgcgatggc cggcaaggac aaggtcgagg tcatcgagat cccggccaag gtcaacgtgg
     6361 tcgccgagta cccggtggcc gtgctcgcga acgcgcccga ggcggcggcg gcacgggcgt
     6421 tcgtcgatct cctcctgtcg gccgacggcc agaagcggct cggcgctgct ggatttgttg
     6481 cgccgtagcc tgctgcgcgg agcgtggccg gccgccgggt acgtgctcgg cgcagccatg
     6541 ctggcgttcc tcgtcgtccc gctcgtcgcg ctgttcgtca ccggcacgcc ggccgactgg
     6601 ctggccggac tgcgccatcc gatcacggcg ccggcgctgc tgctcagcct cacgaccacc
     6661 gcgctcagcc tgggcgtgct cgtcgtgttc ggcacgccgc tggcctggtg gctgggccgg
     6721 agcaacgccc gggcggcgcg gttcgtcgag acggcggtgc agctccccat cgtggtgccc
     6781 ccggccgtgg ccgggctggc gctgctgctc gcgttcggcc ggcgcgggtt gatcgggccg
     6841 ctgctctccg gcctcggcat cgacatcgcg ttctcgacca tcgcggtcgt catcgcgcag
     6901 gtgttcgtgg cggcgccgtt ctacatccag gcggcgaccg ccgcgttcgc cggcatcgac
     6961 gagaacctgc tggtcgtcgc ccggacgctc ggtgcccggc cgggagcggt gctgtttcgc
     7021 gtcgcgctgc cgctcgcgcg tcgcgggctg atcgcgggcg ccgccctggc gtgggcgcgc
     7081 gcgctcggcg agttcggcgc cacgttgatg ttcgccggca acctgaccgg gcggacccag
     7141 acgctgccgc tcgccgtgta cacggcgctc gagagcgacg tgcgcgccgc gcaggcgctc
     7201 gccgggatcc tggtcgtgat cgcgctggcg atcatcgtct tcctgcggcc ccggcgcggt
     7261 cggagccggg gatgaccgcg ctggtcgcga acatcggcgt ccgcgccggc gcgctcgaga
     7321 tcgaggtcgc gctcgaggtc gacgaccgcg gcctggtcct cgtcgggccg aacggcgccg
     7381 gcaagaccac ggtgctgctc gcgctggtcg gtgcgcgccg gcccacccga ggccggatca
     7441 cgatcgcggg cgacgtcctg ttcgacggcg ccgagggtgt cgatcgagcc accgaggagc
     7501 gtggcctggc gtacctgccg caggactacg cgctgtttcc tcacctcacc gccgccgcca
     7561 acgtcgcgtt cggcctgcac ggcccgcgcg ccgagcgcat gcggaaggcc cgcgcgctgc
     7621 tcgaccggct cggcgtcgca gcggtcgccg atcgccaccc cggcgc
//
LOCUS       DASVOR010001147         3754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362369,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001147 DASVOR010000000
VERSION     DASVOR010001147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362369"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(314..1081)
                     /locus_tag="VF516_21930"
     CDS             complement(314..1081)
                     /locus_tag="VF516_21930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEX8110414.1"
                     /translation="MAVQLREVVGYLDATLDVDRFRDYAPNGLQVEGTADVECVVTGV
                     SASVELIGRAVERRADLIVVHHGLVWGAGLTRIAGPLARRLRLLLGNEVSLAAYHLPL
                     DKHPRLGNNAGLCDALGLGAAREPFGDVRGTPLGVAGTWSVPLTRDDAIARIAAGVGK
                     PPFVFPHGPAVVKKVGVCSGAASDLVEAAAAAGCDLFLTGELAERAGDLAKELQITLV
                     AAGHYATEVFGPLRIADELRQRFPGLDVAFVAAPSPL"
     gene            1295..2872
                     /locus_tag="VF516_21935"
     CDS             1295..2872
                     /locus_tag="VF516_21935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEX8110415.1"
                     /translation="MSGIPLQPTYRPEDLTARGWSYEGALGDPGQYPFTRGPHATMYR
                     GKPWTMRMFAGFGSPEDTNRRFKFLLDNGQTGLSTAFDMPTLMGYDPDHARSLGEVGR
                     EGVSVATIDDMTRLFGDIPLDKVSTSMTINAPASVLLALYVAVAEQQGVSPDRLRGTL
                     QNDMLKEFIAQKEWISPIRGHMRIIRDMLVWCSQHMPLWNTISISGYHIREAGATAVQ
                     ELAFTLADGIGYVQLGIDAGLDVDEFAPRLSYFWDVHNDFFEEIAKFRAGRRMWAKIL
                     RERFGAKNPRSWMLRTHAQTAGVSLMAQQPLNNVVRTTMQALAAVLGGTQSLHTNSYD
                     ETYALPTEEAAQLALRTQQVIYEETGVAQVADPLAGSYFVETLTDQVEAAAWEYIHKI
                     DAMGGIVKAVEEGYPQREIARSAFEFQRDVDAGRRPIVGVNKYATPEEGDHIPTLKIE
                     HATETAQIDRVRQFRARRDPAAIDRALAAVREALRDDKVNVMPPILDAVRQRVTLGEI
                     CDLFRSELGVYRDPAYL"
     gene            complement(3026..>3754)
                     /locus_tag="VF516_21940"
     CDS             complement(3026..>3754)
                     /locus_tag="VF516_21940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110416.1"
                     /translation="RARGPDPGDAYDARDWKEEDEPLGTLVAKISPWLAMTNLVKNVG
                     SEVPRASGGTTPWRFDCFEFVIVNRIYAYWRTMSRTEFNLKFNPLTLGYNADMAEKTH
                     LSWEPPILASKPGDKPYRKGETKPTMKDGAVTYEIERIPVDKSWDELVRDAPIGTQII
                     WSNKDTVAKCTKDGTLPFCDYMNENVTKVGQDQYSAHPFGIVSAQRIIDEMATAPFGG
                     DRKKVSKDYVRQNVYISALRYAKK"
BASE COUNT          579 a         1355 c         1233 g          587 t
ORIGIN      
        1 gtcctcgaac atgtccatgc cgtaatgcat gatcgcgagg agcagcgctt ccttggtctt
       61 gatgtggtgg tacagcccgg ccttggtcag tccgcaggcc tcggcgatct cttgcatgct
      121 ggtcgcctcg tacccgcgct ggtgaatcag ccgagccgcg ctctcgagga tctcatggta
      181 ccgctggtcg cgaggacggc tcacatccac cgtgtaacac gaccggcaga tcgcggagca
      241 agcggtttct gtcctaccga tcggtaggtc gcgcggtcag cgctccggca tcgcccggca
      301 tcgcgcgagc gcgttacagc gggctcggcg cggccacgaa cgcgacgtcg agcccgggga
      361 atcgctgacg cagctcgtcg gcgatccgca gcggcccgaa cacctcggtc gcgtaatggc
      421 cggcggcgac cagggtgatc tgcaactcct ttgcaagatc tcccgcgcgc tcggccagct
      481 ctccggtcag gaacagatcg cagccggccg ccgccgcggc ctcgaccaga tcggacgccg
      541 cccccgagca cacgccgacc ttcttgacca cggccggacc gtgcgggaac acgaacggcg
      601 gcttgccgac cccggcggcg atccgcgcga tcgcatcgtc gcgggtcagc ggcaccgacc
      661 acgtgccggc gaccccgagc ggcgttccgc ggacgtcgcc gaacggctcg cgggctgcgc
      721 ccagcccgag cgcgtcgcac agcccggcgt tgttgccgag ccgcgggtgc ttgtcgagcg
      781 gcaggtggta cgcggcgagc gacacctcgt tgccgagcaa gaggcgcagc cgccgcgcca
      841 gcgggccggc gatccgggtc aaccccgccc cccacaccag cccgtggtgg acgacgatca
      901 ggtcggcgcg gcgctcgacc gcgcggccga tcagctcgac cgacgccgac accccggtga
      961 ccacgcactc gacgtccgcg gtgccctcga cctgcagccc gttgggcgcg tagtcgcgga
     1021 accgatcgac gtccagcgtg gcgtccaggt acccgacaac ctcgcgcagc tgcaccgcca
     1081 tcgcgcacga tcgtcctcga gaccgcgcgc cgggtcaacg gcttgccgcg gtgtggcgaa
     1141 accgtcgcgg ccggcccgat ctgtgccgac tgtacggtca tcggcgccac ggctcccgtg
     1201 gtacaccgct gccatggccc cggtgaagaa gcccgaagcg gtgattgtcg cccccggtcg
     1261 ggcgtccccc gacgaagtcc gtacggtccg taccatgagc gggatcccgc tccagccgac
     1321 ctaccgcccc gaggacctca cggcgcgcgg ctggagctac gagggcgcgc tcggcgaccc
     1381 gggccagtac ccgttcaccc gcggcccgca cgccacgatg taccgtggca agccgtggac
     1441 gatgcggatg ttcgccgggt tcggctcgcc cgaggacacc aaccgacggt tcaagttcct
     1501 gctcgacaac ggccagaccg gcctgtcgac cgcgttcgac atgccgacgc tgatgggcta
     1561 cgaccccgac cacgcgcgct cgctcggcga ggtcggccgc gagggcgtgt cggtcgcgac
     1621 gatcgacgac atgacccggc tgttcggcga catcccgctc gacaaggtct cgacatcgat
     1681 gacgatcaac gcgccggcct cggtcctgct cgcgctctac gtcgcggtcg ccgagcagca
     1741 gggcgtgtcg cccgaccggc tgcgcggcac gctgcagaac gacatgctca aggagttcat
     1801 cgcgcagaag gagtggatct cgccgatccg cggccacatg cggatcatcc gcgacatgct
     1861 cgtgtggtgc tcccagcaca tgccgctgtg gaacacgatc tcgatcagcg gctaccacat
     1921 ccgcgaggcg ggcgcgaccg cggtccagga gctcgcgttc accctcgccg acggcatcgg
     1981 ctacgtccag ctcggcatcg acgccgggct cgacgtcgac gagttcgcgc cgcggctgtc
     2041 gtacttctgg gacgtccaca acgacttctt cgaggagatc gccaagttcc gcgccggccg
     2101 gcggatgtgg gccaagatcc tgcgcgagcg gttcggcgcc aagaacccgc ggtcgtggat
     2161 gctgcgcacc cacgcccaga ccgccggcgt gtcgctgatg gcgcagcagc cgctcaacaa
     2221 cgtcgtgcgc accaccatgc aggcgctcgc cgcggtgctc ggcggcaccc agtcgctcca
     2281 caccaacagc tacgacgaga cctacgcgct tccgaccgag gaggccgccc agctcgcgct
     2341 gcgcacccag caggtgatct acgaggagac cggcgtcgcc caggtcgccg atccgctcgc
     2401 cggcagctac ttcgtcgaga ccctgaccga tcaggtcgag gccgccgcgt gggagtacat
     2461 ccacaagatc gacgccatgg gcggcatcgt caaggcggtc gaggagggct acccgcagcg
     2521 cgagatcgcg cgctcggcgt tcgagttcca gcgcgacgtc gacgccggcc gccgtccgat
     2581 cgtcggcgtc aacaagtacg ccacccccga ggagggcgac cacatcccga ccctcaagat
     2641 cgagcacgcc accgagaccg cgcagatcga ccgggtccgc cagttccgcg cccggcgcga
     2701 ccccgccgcg atcgaccgcg cgctggccgc cgtgcgcgag gcgctgcgcg acgacaaggt
     2761 caacgtgatg ccgccgatcc tcgacgcggt caggcagcgc gtgacgctcg gcgagatctg
     2821 cgacctgttc cgctccgagc tcggcgtcta ccgcgatccg gcgtacctgt aggtggaaca
     2881 ccgtgggtcc gttcctccgc gaagcccccc ggtgtggggc gtccgtcgcc gggtgtcctt
     2941 gcgcggcgag cgcccgcacc gcgcgtacga caggacgatc gacagcaggc acaccgcgcg
     3001 agccggaaga cggcccgcgc gccggctact tcttcgcgta gcgtagggcc gagatataga
     3061 cgttctgccg tacatagtcc ttggagacct tcttccggtc gccgccgaag ggcgcagtgg
     3121 ccatctcgtc gatgatcctc tgcgcgctca cgatcccgaa cgggtgcgcc gaatattggt
     3181 cctgccccac cttcgtcacg ttctcgttca tatagtcgca gaatgggagg gtcccatcct
     3241 tggtgcattt ggcgacggtg tccttgttgc tccagatgat ctgtgtcccg atgggcgcgt
     3301 cacgcacgag ctcgtcccag gatttgtcga cggggatcct ctcgatctcg taggtcacgg
     3361 ccccgtcctt catcgtgggc ttcgtctcgc ctttccggta cggcttgtcc ccgggcttgc
     3421 tggccaggat cgggggctcc cacgacaggt gggttttctc ggccatgtcg gcgttgtagc
     3481 ccagggtcaa ggggttgaac ttcaggttga actcggtgcg cgacatcgta cgccaatagg
     3541 catagatgcg gttcacgatc acgaactcga agcaatcgaa tctccacggt gtcgtgccgc
     3601 cgctcgcgcg cggcacctcg gagccgacgt tcttcacgag gttcgtcatg gcgagccagg
     3661 ggctgatctt ggcgaccagg gtgccgaggg gctcgtcctc ttccttccag tctcgcgcgt
     3721 cgtaggcgtc gcccgggtcc ggtcctctcg cgcg
//
LOCUS       DASVOR010001148         4825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_479290,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001148 DASVOR010000000
VERSION     DASVOR010001148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4825
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479290"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="VF516_21945"
     CDS             complement(<1..962)
                     /locus_tag="VF516_21945"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8110417.1"
                     /translation="MNGAPARGIALIVLLATCTVGPAYHVPSAPLPGTPAYKEAARAG
                     PWRIASPADAMLRGAWWRVFREPELDALEDQLRTGNQTIAQAVANFMAARAVIREAQA
                     QYYPTVTASPSVITGRGGGGSLATGTLTPTGATGVTGTTGVPGTIGTTGTGTTGTGTT
                     GTGTTGTTGTPGTRGTAAAAAAGGGRFTSYSLPIDAAWAPDLFGRVRNTVRQARYSAQ
                     VSAADLAGQELLEQATLAATYFQLRGQDALQELLDATVAADEQILALARARYETGVDT
                     EVAVVQAEQTLQAARVQATGAGILRAQYEHAIATLIGRPATAFSL"
     gene            complement(959..4018)
                     /locus_tag="VF516_21950"
     CDS             complement(959..4018)
                     /locus_tag="VF516_21950"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8110418.1"
                     /translation="MNLSAPFIRRPVGTILLSAALVIAGAVAFAVLPVSPLPRIDFPT
                     ISVAAALPGASAEIMAATVATPLERQLTRIAGITEMTSTSTLGTTVVTLQFDLGRDID
                     GAARDVQAAINAARTYLPANLPTDPTYRKVNPADAPILIVSLTSDSYRLGQMYDVAST
                     VIAQRLSQIPGVGQVTVGGASSPAIRVEVDPVNLASNGLSLADVRAAIQQQNTSLARG
                     QLVDGPVVADILTNGQIVRAEDYRSLVIEVQGQRAVRLSDVADVVDGQQNARSAGYLD
                     GKPSVTLIIFREPGANIIATVDRVRAAMPAIAATVPRGIDLTIAVDRTTTIRASVRTV
                     EHTLLLSVALVVLVVFVFLRSARATAIPSIAVPTSLIGTFAVMYLFGFSLDNLSLMAI
                     TIATGFVVDDAIVVMENIARHREAGVPAFEAALRGAREIGFTVLSISVSLVAVFTPIL
                     AMQGLVGRLFREFAVTLSIAIVISMVVSLTVTPMLCAHLLGAERAPRHRGILRLFEGG
                     FDGLLAGYRRSLTWVLDNRGLMIVLFALAIAMNAVLVVRVPKGFFPEQDTGVVFGAVL
                     GPDDASFASMDASQRMIVDAIRRDPAVDHVLAFNQTDSAGLVFMSLVPREQRDASAPQ
                     LIARLRRTLSRLPAASVFLQAAQDLRVGGRISAAQYQYTIQSDTLADLQTWGPILLAR
                     MRQLPGFTDVSSDQHNGGLQAFLTYDRARAAALGLTALQLDTELASAFCQAQVSVMYL
                     PANQYYVVLEAAPPWTQAPARLADVYLPTTPPGGQVPLVGVASPRITTAALQVNHTGL
                     FPSVTVSFNLAPGLALGDAARRIAELSARLGTPATIRGSFSGTLQAFQQSLASEPLLI
                     ATALLAVYLVLGILYESLIHPLTILSTLPSASVGAMLALLVFGLDLDVISIIGIVLLI
                     GIVKKNAIMMIDFALLAERDHGKPPREAIFEACLLRFRPIMMTTIAALSGALPLAFGT
                     GTGSELRRPLGIAIVGGLVMSQLLTLYTTPVIYLYLDRLRRGRRRAR"
     gene            complement(4015..>4825)
                     /locus_tag="VF516_21955"
     CDS             complement(4015..>4825)
                     /locus_tag="VF516_21955"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8110419.1"
                     /translation="IDSLDRIYVRSGTGAAIPLSAFATLETRSTALAIDHQGQFPAVT
                     VSFNLAPGVSLGTALASIQRAVDDLDLPASVQTGFQGTAAAFLASLTNEPLLILAALV
                     TVYIVLGVLYESFVHPLTILSTLPSAGVGALLALLIFRQDLDVIAIIGILLLIGIVKK
                     NGIMIVDFALEAERERGLAPDDAVYEASLLRFRPILMTTMAALFGGLPLALGGGLGAE
                     LRRPLGIAMVGGLLVSQVLTLYTTPVIYIAFEALSRRVRARRRREPSRGAG"
BASE COUNT          708 a         1720 c         1772 g          625 t
ORIGIN      
        1 agcgagaatg cggtcgccgg ccggccgatc agcgtggcga tcgcgtgctc gtactgcgcg
       61 cgcaggatgc cggcgccggt cgcctggacg cgcgcggcct gcagggtctg ctcggcctgg
      121 acgaccgcga cctcggtgtc gaccccggtc tcgtagcgcg cgcgggccag cgcgaggatc
      181 tgctcgtcgg cggcgaccgt ggcgtcgagc agctcctgca gcgcgtcctg gccgcgcagc
      241 tggaagtacg tggcggccag cgtcgcctgc tccagcagct cctggcccgc gaggtccgcc
      301 gcgctgacct gcgcgctgta ccgggcctgg cgcaccgtgt tgcgcacgcg gccgaacagg
      361 tccggggccc aggccgcgtc gatcggcagg ctgtagctgg tgaaccggcc gccgccggcc
      421 gccgccgcgg ctgcggtacc gcgggtcccg ggggttcccg tcgtcccggt cgttcccgtc
      481 ccggtcgttc ccgtcccggt cgttcccgtc ccggtcgttc cgatcgttcc tggaactccg
      541 gtggtccccg tgacgcccgt cgcgccggtc ggcgtgagcg tgccggtcgc cagcgagccg
      601 cctccgcccc gccccgtgat caccgagggc gaggcggtga cggtcgggta gtactgcgcc
      661 tgcgcctcgc ggatcacggc ccgcgcggcc atgaagttcg cgaccgcctg ggcgatcgtc
      721 tggttgccgg tgcggagctg gtcctcgagc gcgtcgagct ccggctcgcg gaacacgcgc
      781 caccacgcgc cgcgcagcat cgcgtcggcc gggctggcga tccgccacgg accggcgcgc
      841 gcggcctcct tgtacgccgg cgtccccggc agcggggccg acggcacgtg gtacgccggg
      901 cccaccgtgc acgtcgcgag cagcacgatc agcgcgatgc cccgcgccgg cgctccgttc
      961 atcgcgccct ccggcggccg cggcgcagcc ggtcgaggta gaggtagatc accggcgtgg
     1021 tgtagagcgt gagcagctga ctcatcacca gcccgccgac gatcgcgatg ccgagcggcc
     1081 ggcgcagctc ggagccggtt ccggtcccga acgccagcgg cagcgcgccg gacagcgccg
     1141 cgatggtcgt catcatgatc ggccggaacc gcagcaggca ggcctcgaag atcgcctcgc
     1201 gcggcggctt gccgtggtcg cgctcggcga gcagcgcgaa gtcgatcatc atgatcgcgt
     1261 tcttcttgac gatcccgatc agcagcacga tcccgatgat cgagatcacg tcgaggtcga
     1321 gcccgaacac cagcagcgcc agcatcgcgc cgacgctcgc cgacggcagc gtcgacagga
     1381 tggtcagcgg gtggatcagg ctctcgtaga ggatgccgag cacgaggtag accgcgagca
     1441 gcgcggtggc gatcagcagc ggctcggagg cgagcgactg ctggaacgcc tgcagcgtcc
     1501 ccgagaacga gccgcggatc gtcgccggcg tgccgagccg ggcggagagc tcggcgatcc
     1561 gccgcgccgc gtcgccgagc gcgagccccg gggcgaggtt gaacgacacc gtgaccgacg
     1621 ggaacagccc ggtgtggttg acctgcagcg cggcggtcgt gatccgcggc gacgcgacgc
     1681 cgaccagcgg gacctgtccg cccggcggcg tcgtcggcag gtagacgtcg gccaggcggg
     1741 ccggcgcctg ggtccacggc ggcgcggcct cgagcacgac gtagtactgg ttcgcgggga
     1801 ggtacatcac cgacacctgg gcctggcaga acgcgctggc gagctcggtg tcgagctgca
     1861 gcgcggtcag cccgagcgcc gcggcgcgcg cccggtcgta ggtcaggaac gcctgcagcc
     1921 cgccgttgtg ctgatccgag ctgacgtcgg tgaacccggg cagctgccgc atccgggcca
     1981 ggaggatcgg cccccaggtc tggagatcgg cgagcgtgtc ggactggatc gtgtactggt
     2041 actgcgcggc gctgatccgg ccgccgaccc ggaggtcctg cgcggcctgg aggaacaccg
     2101 aggccgccgg caggcgggac agcgtgcggc gcagccgggc gatcagctgc ggggcagagg
     2161 cgtcgcgctg ctcgcgcggg accagcgaca tgaacaccag gccggcgctg tcggtctggt
     2221 tgaatgccag gacgtggtcg accgccggat cgcgccggat cgcgtcgacg atcatccgct
     2281 gcgatgcgtc catcgatgcg aacgaggcgt cgtcggggcc gagcacggcg ccgaacacca
     2341 cgccggtgtc ctgctcgggg aagaacccct tcggcacgcg gaccacgagc acggcgttca
     2401 tcgcgatcgc cagcgcgaac agcacgatca tgaggccgcg gttgtcgagc acccaggtca
     2461 ggctgcgccg gtagcccgcg agcaggccgt cgaacccgcc ctcgaacagc cgcaggatcc
     2521 cgcggtgccg cggcgcgcgc tcggcgccca gcaggtgggc gcacagcatc ggcgtcacgg
     2581 tcagcgacac caccatcgag atcacgatcg cgatcgacag cgtgaccgcg aactcgcgga
     2641 acagccggcc gaccaggccc tgcatcgcca ggatcggcgt gaacaccgcg accagcgaca
     2701 cgctgatcga cagcacggtg aacccgatct cgcgcgcgcc gcgcagcgcc gcctcgaagg
     2761 cgggcacccc ggcctcgcgg tggcgcgcga tgttctccat cacgacgatc gcgtcgtcga
     2821 ccacgaaccc ggtcgcgatc gtgatcgcca tcagcgacag gttgtcgagg ctgaacccga
     2881 acaggtacat caccgcgaac gtgccgatca gcgaggtcgg caccgcgatg ctcgggatcg
     2941 cggtcgcgcg cgcgctgcgc aggaacacga acacgaccag gacgaccagc gcgaccgaca
     3001 gcagcagggt gtgctcgacg gtgcggaccg acgcgcggat cgtcgtggtc cgatcgaccg
     3061 cgatcgtcag gtcgatccca cgcggcacgg tcgcggcgat cgccggcatc gccgcgcgca
     3121 cccggtcgac cgtcgcgatg atgttcgcgc ccggctcgcg gaagatgatc agcgtcaccg
     3181 acggcttgcc gtcgaggtag ccggcgctgc gcgcgttctg ctggccgtcg acgacgtcgg
     3241 cgacgtcgga cagccgcacc gccctctggc cctgcacctc gatcacgagc gaccggtagt
     3301 cctcggcgcg cacgatctgg ccgttggtca ggatgtcggc gaccaccggc ccgtcgacca
     3361 gctggccgcg ggcgaggctg gtgttctgct gctggatcgc cgcgcgcacg tccgccaggc
     3421 tcaggccgtt gctcgcgagg ttcaccgggt cgacctcgac ccggatcgcc ggcgacgacg
     3481 cgccgccgac cgtgacctgg ccgaccccgg ggatctgcga cagccgctgg gcgatcaccg
     3541 tcgaggcgac gtcgtacatc tgcccgagcc ggtagctgtc cgaggtcagg ctgacgatca
     3601 ggatcggcgc atcggccggg ttgaccttgc ggtaggtcgg atcggtcggc aggttggcgg
     3661 gcaggtaggt gcgcgccgcg ttgatcgccg cctggacgtc gcgcgccgcg ccgtcgatgt
     3721 cgcggccgag atcgaactgc agcgtgacca ccgtggtgcc cagcgtgctg gtcgacgtca
     3781 tctcggtgat cccggcgatc cgcgtgagct ggcgctcgag cggggtcgcg acggtcgccg
     3841 ccatgatctc ggcgctcgcg cccggcagcg cggcggccac gctgatcgtc gggaagtcga
     3901 tccggggcag cggcgacacc ggcagcaccg cgaacgccac cgcgccggcg atcaccagcg
     3961 ccgccgacag caggatcgtc ccgaccggcc gccggatgaa cggcgccgac aggttcatcc
     4021 tgccccccgg ctcggctccc ggcgccggcg ggcgcgcacc cggcgcgaca gcgcctcgaa
     4081 cgcgatgtag atgaccggcg tggtgtagag cgtcaggacc tggctgacca gcaggccgcc
     4141 gaccatcgcg atgccgagcg ggcggcgcag ctcggcgccg aggcccccgc cgagcgcgag
     4201 cggcaggccg ccgaacagcg ccgccatcgt cgtcatcagg atcggccgga accggagcag
     4261 gctcgcctcg tacaccgcgt cgtcgggggc aagcccgcgc tcgcgctcgg cctcgagcgc
     4321 gaagtcgacg atcatgatgc cgttcttctt gacgatgccg atcaacagca ggatcccgat
     4381 gatcgcgatg acgtcgagat cctggcggaa gatcagcagc gcgagcagcg cgccgacgcc
     4441 cgccgacggc agggtcgaca ggatcgtcag cgggtggacg aagctctcgt acagcacgcc
     4501 gagcacgatg tagaccgtga ccagcgccgc gaggatcagc agcggctcgt tggtcagcga
     4561 tgcgaggaac gcggcggcgg tgccctggaa gccggtctgc acgctcgccg ggaggtcgag
     4621 gtcgtcgacg gcgcgctgga tgctcgccag cgcggtgccg agcgacaccc ccggcgcgag
     4681 attgaacgac accgtcaccg ccgggaactg gccctggtgg tcgatcgcga gcgcggtgct
     4741 gcgggtctcc agcgtggcga acgccgacag cgggatcgcg gcgccggtcc ccgaccgcac
     4801 gtagatccga tcgagcgagt cgata
//
LOCUS       DASVOR010001149         3329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_128322,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001149 DASVOR010000000
VERSION     DASVOR010001149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3329
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128322"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VF516_21960"
     CDS             <1..500
                     /locus_tag="VF516_21960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110420.1"
                     /translation="AARVPPAPAAAYVPPMQAQHAAAPAHVAAAPSNPELRTTPLPRP
                     RSRTSRHPPLTHARTALGYTSGAGAQSAIVRLGLAPHVSARLGRLVDRVVPGDFHLDG
                     RERRVLNALGEGELTARGIGHMLDIADPVSFMEELTRKLEAYNLDLVEPGDPQGGEPT
                     YRLRA"
     gene            579..1961
                     /gene="tig"
                     /locus_tag="VF516_21965"
     CDS             579..1961
                     /gene="tig"
                     /locus_tag="VF516_21965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEX8110421.1"
                     /translation="MQVRIEDVSPVEKKMFVEVPWETVSQKLGDAYKELGKGVALKGF
                     RKGKVPRPVLEQVYGPRVNAEVAYQLVRESFVRASEEHNLAAVAEPRVEEAAPIKKGQ
                     PFTFAAIIEIRGDVVPQDYVGLAIERRKLAIPESAVDEALAQLRREHTELRPIEGRDV
                     TAPGDIVGLSVTGTIGEHQINQPQFAVDLDDAEREPLPGMREALTGVPLDTKDKKIEI
                     TLPDDYKDESIRGRKAELTVTILEVRAKEVPALDDEFAKDTGKADTLDGLRAALRKEL
                     EDREREVIDREARENVLRELIKKNQIPVAGSLVDRAVEMQYARLRQMLGMKPERGNPG
                     LNDELRDKLRPAGADEVRGQLVLEAIADKEGLTITDDELARHIEVTARGRNVAPAKLR
                     AEWQRDNRIDNVVYSLRQDKVLRFLVDKAQVTEVEKLSQQGTPLPEAPALHDHDEPGH
                     VHGPDCDHDH"
     gene            2063..>3329
                     /locus_tag="VF516_21970"
     CDS             2063..>3329
                     /locus_tag="VF516_21970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110422.1"
                     /translation="MQSDSTEIAASGEPHPSLSELKNSSHPKISRHLAHCSMCRSVVA
                     TDEELGDPIPLDLPKGLVSENAFRWPADTMARGGMAQIFAGEDRRLARTVILKAPREG
                     DDLPAGMAEMFQRRVTAEARILAKLQHPSIVTIYELGKSSAGWPFCVLEKVDGVSLRD
                     RLDELADAEADGRPRTRERLELLSSLVSIAEALAYAHERRVVHRDCTPNNILLGKRGE
                     ATLIDWGIARDLDAPGGATDPALLGGEAPVHGGETISAGTPPYLPLEQTQGRHADPSF
                     DVYSFGVTLYEVVAGRTPFDWTHVSAPDARTRQLAAFIDWLRAGPPAPPAMPRDPELS
                     GIIARAMARSPAERFTADELVRALKQYLTGDLVFSHRYSTTGRLARWARRHRGAAVAL
                     VSVALFAIGGALVWAQLSRRAQKEAELQAI"
BASE COUNT          510 a         1234 c         1165 g          420 t
ORIGIN      
        1 gcgcggcgcg ggtcccgccg gccccggccg ccgcctacgt gccgccgatg caggcgcagc
       61 atgccgcggc gcccgcccac gtcgccgccg cgccgtccaa ccccgagctg cgcaccacgc
      121 cgctgccgcg cccacggagc cggacctctc gccacccgcc gctgacccac gcccggaccg
      181 cgctcggcta cacctccggc gcgggcgcgc agtcggcgat cgtccggctc gggctcgctc
      241 cgcacgtctc ggcgcggctc ggccggctgg tcgaccgggt cgtgcccggc gacttccacc
      301 tcgacggccg cgagcgccgt gtgctcaacg ccctcgggga gggggagctc accgcgcgcg
      361 gcatcggcca catgctcgat atcgccgatc cggtctcctt catggaagag ctgacccgca
      421 agctcgaggc ctacaacctc gacctggtcg agcccggcga tccgcaaggc ggtgagccga
      481 cctaccgcct gcgagcgtga gctctcgacc gactcccgga acgggtcatc cgcaggcgtt
      541 tcgcgactgg cttggctgtg gtaaccacgc ctgccgccat gcaggtacgc atcgaagatg
      601 tttcaccggt cgagaagaag atgttcgtcg aggtcccgtg ggagaccgtg agccagaagc
      661 tcggcgacgc gtacaaggag ctcggcaagg gcgtggcgct caaggggttt cgcaagggca
      721 aggtcccgcg cccggtgctc gagcaggtct acggcccccg ggtcaacgcc gaggtcgcct
      781 accagctggt ccgcgagtcg ttcgtgcgcg cgtccgagga gcacaacctc gccgcggtcg
      841 ccgagccgcg ggtcgaggag gcggcgccga tcaagaaggg ccagccgttc acgttcgccg
      901 cgatcatcga gatccgcggc gacgtcgtgc cccaggacta cgtcggcctg gcgatcgagc
      961 gccgcaagct cgcgatcccc gagtccgcgg tcgacgaggc gctcgcccag ctccgccgcg
     1021 agcacaccga gctccggccg atcgagggcc gcgacgtcac cgcgccgggc gacatcgtcg
     1081 ggctcagcgt caccggcacg atcggcgagc accagatcaa ccagccgcag ttcgcggtcg
     1141 acctcgacga cgccgagcgc gagccgctgc ccggcatgcg cgaggcgctg accggcgtgc
     1201 cgctcgacac caaggacaag aagatcgaga tcaccctccc cgacgactac aaggacgagt
     1261 cgatccgcgg ccgcaaggcc gagctgacgg tcaccatcct cgaggtccgc gccaaggagg
     1321 tcccggcgct cgacgacgag ttcgccaagg acaccggcaa ggccgacacc ctcgacggcc
     1381 tgcgcgcggc gctgcgcaag gagctcgagg accgcgagcg cgaggtcatc gaccgcgagg
     1441 cccgcgagaa cgtgctgcgc gagctgatca agaagaacca gatcccggtc gccgggtcgc
     1501 tcgtcgatcg cgccgtcgag atgcagtacg cgcggctgcg ccagatgctc ggcatgaagc
     1561 ccgagcgcgg taaccccggt ctcaacgacg agctgcgcga caagctgcgc ccggccggcg
     1621 ccgacgaggt ccgcggccag ctcgtgctcg aggcgatcgc cgacaaggaa ggcctgacga
     1681 tcaccgacga cgagctcgcc aggcacatcg aggtcaccgc ccggggccgc aacgtcgccc
     1741 cggccaagct gcgcgccgag tggcagcgcg acaaccggat cgacaacgtc gtgtactcgc
     1801 tgcgccagga caaggtgctg cgcttcctgg tcgacaaggc ccaggtcacc gaggtcgaga
     1861 agctgtccca gcagggcacc ccgctgcccg aggcgccggc cctccacgac cacgacgagc
     1921 ccggccacgt ccacggcccc gactgcgacc acgaccactg acggcaagtt cttgcgctga
     1981 cgagccagcg gttctcgcag cgtccggcgg ggcgagtgaa cttcatgcgg tgttctcggc
     2041 ggtgcgcgtt aagatcgccg ccatgcaaag cgattcgacg gagatcgccg ccagcgggga
     2101 gccgcatccg agcctctccg agctgaagaa ctcgtcgcat ccgaagatca gccggcacct
     2161 ggcgcactgc tcgatgtgcc ggtcggtggt cgccaccgac gaggagctcg gcgacccgat
     2221 cccgctcgat ctgcccaagg ggctggtcag cgagaacgcg ttccgctggc ccgccgatac
     2281 gatggcgcgc ggcggcatgg cgcagatctt cgccggcgag gatcgccggc tcgcccgcac
     2341 cgtgatcctc aaggcgccgc gcgagggcga tgacctgccg gcggggatgg cggagatgtt
     2401 ccagcgccgg gtgaccgccg aggcgcggat cctggccaag ctgcagcatc cgtcgatcgt
     2461 gacgatctac gagctcggca agtcgtcggc cggctggccg ttctgcgtgc tggagaaggt
     2521 cgacggcgtg tcgctgcgcg accggctcga cgagctcgcc gacgcggagg ccgacggccg
     2581 gccgcggacc cgcgagcggc tcgagttgct gtcgagcctg gtctcgatcg ccgaggcgct
     2641 ggcctacgcc cacgagcggc gcgtcgtgca ccgcgactgc acgccgaaca acatcctgct
     2701 cggcaagcgc ggcgaggcga cgctgatcga ctggggcatc gcgcgcgatc tcgacgcgcc
     2761 cggcggcgcg accgatccgg cgctgctcgg cggcgaggcg ccggtgcatg gcggcgagac
     2821 gatcagcgcc ggcacgccgc cctacctgcc gctggagcag acccagggcc ggcacgccga
     2881 tccgagcttc gatgtctaca gcttcggggt cacgctgtac gaggtcgtcg ccggccggac
     2941 gccgttcgac tggacccacg tctcggcgcc cgacgcacgg acccggcagc tcgcggcgtt
     3001 catcgactgg ctccgggccg ggccgcccgc gccgccggcg atgcctcgcg atccggagct
     3061 gtcggggatc atcgcgcgcg cgatggcgcg ctcgccggcc gagcggttca ccgccgacga
     3121 gctggtccgt gcgctcaagc agtacctgac cggcgacctg gtgttctcac accgctactc
     3181 gacgaccggc cggctcgcgc gctgggcccg ccgccaccgc ggcgccgccg tggcgctggt
     3241 ctcggtcgcg ctgttcgcga tcggcggcgc gctggtctgg gcccagctgt cgcgccgcgc
     3301 ccagaaggag gccgagctcc aggcgatcg
//
LOCUS       DASVOR010001150         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_391141,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001150 DASVOR010000000
VERSION     DASVOR010001150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391141"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="VF516_21975"
     CDS             <1..593
                     /locus_tag="VF516_21975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110423.1"
                     /translation="TGDARTVKPAAPAEPPHAAPATTHKATTATASAQPRTPSPAKPD
                     KAAAAPPPPKDTPAPKADKSGGGDCDEVSCVLNSYEGACCAKFRKGGGKSAPASGGSG
                     PKAGGSDLPDSLDRAMIADGVAKVKQKAQACGDKSPAKGQVKVSVKVSPDGNVTSVAV
                     KNTPDPGLGNCVAGVMQTAKFSRTQSGGSFAYPFQF"
     gene            698..>1838
                     /locus_tag="VF516_21980"
     CDS             698..>1838
                     /locus_tag="VF516_21980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8110424.1"
                     /translation="MSDLETQLSPRTGEHIVSTLRVEVVDGPDRGKFVVGGDVVSVGT
                     ARDNALPVADFTVSRYHLDVSVKPGGILVTDLGSTNGTYIGAVRIERAIVPPGTLLRL
                     GGTTIRFDDAVRRTVPAPGAGAPRELAGMIAVSPQMLRLFADIERIAATPTSVLIVGE
                     SGTGKERVAQALHARSPRAGAPLVTIDCGALSSSLLASELFGHERGAFTGADRQHKGA
                     FERAEDGTVFLDEIGELPGADQSALLGVLERRRFRRVGGSAELDLEARVIAATNRDLR
                     AEVNTGRFRQDLYHRLAVVVLRLAPLRERREDIPVLVEHFARELGATGGVEALFGADQ
                     LARWQKHPWPGNVRELRNAVEAALVVGPQPTTGDVPEPAGPGLAPV"
BASE COUNT          257 a          663 c          702 g          216 t
ORIGIN      
        1 cgaccggcga tgccaggacc gtcaagccgg cggctcccgc cgagcctccg catgcggcgc
       61 cggcgacgac gcacaaggcg acgaccgcga cggcctcggc gcagcccagg acgccgtcgc
      121 cggccaagcc cgacaaggcc gcggcggcgc ccccgccgcc gaaggacaca ccggcgccca
      181 aggccgacaa gagcggcggc ggtgactgcg acgaggtgtc gtgcgtgctc aacagctacg
      241 agggcgcgtg ctgcgccaag ttccgcaagg gcggcggcaa gtcggcgccg gcgagcggcg
      301 gctcgggccc caaggccggc ggcagcgacc tgcccgacag cctcgatcgc gcgatgatcg
      361 ccgacggcgt cgccaaggtg aagcagaagg cccaggcgtg cggcgacaag tcgcctgcca
      421 agggccaggt caaggtgtcg gtgaaggtca gtcccgacgg caacgtcacc agcgtcgcgg
      481 tgaagaacac gccggatccg ggcctgggca actgcgtcgc cggcgtgatg cagaccgcga
      541 agttctccag gacccagagc ggcggctcgt tcgcgtatcc gttccagttc tgacggccgg
      601 caagcccggg gccctgtgcc acggcggatc gccgcgcgct gtgcttaact gacggccggg
      661 ctgtcgggtc cgaccgacag tgatgaaacc tgccgtcatg agcgatctcg agacccagct
      721 gtcgccgcgg accggcgagc acatcgtgtc gacgctgcgg gtcgaggtcg tcgacggccc
      781 ggacaggggc aagttcgtgg tcggcggcga tgtggtctcg gtcggcaccg cgcgcgacaa
      841 cgcgctgccg gtcgccgact tcacggtgtc gcgctaccac ctcgacgtca gcgtcaagcc
      901 gggcgggatc ctggtgaccg acctcggctc gaccaacggc acgtacatcg gcgcggtgcg
      961 gatcgagcgg gcgatcgtgc cgcccggcac gctgctccgg ctcggcggca cgacgatccg
     1021 gttcgacgac gcggtccgcc gcaccgtacc ggcgccggga gcgggggcgc cccgcgagct
     1081 cgccggcatg atcgcggtgt cgccgcagat gctgcggctg ttcgccgaca tcgagcggat
     1141 cgccgcgacg ccgacctcgg tgctgatcgt cggcgagtcg ggcaccggca aggagcgcgt
     1201 ggcccaggcg ctgcacgcgc ggtcaccgcg cgccggcgcg ccgctggtga cgatcgactg
     1261 cggcgcgctg tcgtcgagcc tgctcgcgag cgagctgttc ggccacgagc gcggcgcgtt
     1321 caccggcgcc gaccgccagc acaagggcgc gttcgagcgc gccgaggacg gcacggtgtt
     1381 cctcgacgag atcggcgagc tgccgggcgc cgaccagagc gcgctgctcg gcgtgctcga
     1441 gcgccggcgg ttccgccggg tcggcggctc ggccgagctc gacctcgagg cccgcgtgat
     1501 cgcggcgacc aaccgcgacc tgcgcgccga ggtcaacacc ggccggttcc gccaggacct
     1561 ctaccaccgg ctcgcggtcg tggtgctgcg gctggcgccg ctgcgcgagc gccgcgagga
     1621 catcccggtg ctggtcgagc acttcgcgcg cgagctcggc gccacggggg gcgtcgaggc
     1681 gctgttcggc gccgaccagc tggcgcgctg gcagaagcac ccgtggccgg gcaacgtgcg
     1741 cgagctgcgc aacgcggtcg aggccgcgct ggtcgtcggg ccgcagccga ccaccggcga
     1801 cgtgccggag ccggctgggc cggggctcgc gccggtgg
//
LOCUS       DASVOR010001151         5882 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_12399,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001151 DASVOR010000000
VERSION     DASVOR010001151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5882
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12399"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VF516_21985"
     CDS             complement(<1..1047)
                     /locus_tag="VF516_21985"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8110425.1"
                     /translation="MVDLDFRALFEAAPGLYLVLAPDPGLTILAASDAYLRATMTERA
                     AIVGRGLFDVFPDNPADPEATGTRNLSASIARAIATREPDTMAVQKYDVRRPDGTFEQ
                     RWWSPVNTPVLDGDHAVRYILHRAEDVTELAHARREGVALEARIAAEQQRADLRFRDL
                     VDLAPEGVIVCDAESTVLLVNVAAERMFGFARTELIGHPVEMLIPERARPRHAAHIAR
                     FAAAPSTRPMGAGRELAGLRKDGSEFPVEISLSPMRTAGALTILTAIRDITERRKIER
                     DLERLAAIVDSSEDAIVGETLGGDVTSWNISAERMFGYTAREMLGQSIAKLLPGGRLD
                     EEGAMLARIAHGEKV"
     gene            complement(1154..2797)
                     /locus_tag="VF516_21990"
     CDS             complement(1154..2797)
                     /locus_tag="VF516_21990"
                     /inference="COORDINATES: protein motif:HMM:NF036398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110426.1"
                     /translation="MKRQLAVLVGLFVASASSAAFAWDAESRDSVGGKAEWRGNTHLF
                     IVEKAIDLLKHSDDPFALGAARFLDACTQEWRQGLWDADGRDELVDSNNHGSHFYDPS
                     PSDHCHTYALALSSCVSGSFDARQNARNQIQHFATGKSAVPAKTYGHHAKSRCYALGL
                     ALHYMTDVTMPYHAAQYSGASPPLFLHPTLEAYVPVIQHRFVQTSWTPGAGSPDDVLV
                     AAAKGGKALFGSFKKAINDAGGACVYVSGAGGVYAGPCFRNKPAVDAAIGEALKRAQL
                     NTATYLHAVFRASPSLIPPGPAQFAAIWVKQAGPDWIARHGMTSQQYQQEFDKQTQQG
                     FCLVDVTGYEEAGQPRFAAIWERKGCAGIVARHGMTAQDYQQAFDSLGKQGFRLTLIN
                     GYTVAGQDRYAAIWDRSPGPDFVARHGMTAQDYQQAFDSLGKQGWCLSQVSGTSVAGQ
                     DRYAAIWDRKPCPTFVARHGMTAPQYQQAFDELGRQGFRLRLVSAYQINGEPAYAAIW
                     DKSPGPSFVARHGMTPDQYQKAFDQLTKDGFRLVWIDAD"
     gene            3061..3441
                     /locus_tag="VF516_21995"
     CDS             3061..3441
                     /locus_tag="VF516_21995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110427.1"
                     /translation="MAARKSLMILIAGPYRSGTGDDPGKLAAHVAAMEALALPVFRAG
                     HVPVVGEWLALPLCRLAGSTQVGDAAFHEVFHPIAERLLEHCDGVLRVGGASQGADLM
                     VATAERLGLAVYRRLEDIPGCSAT"
     gene            3596..4363
                     /locus_tag="VF516_22000"
     CDS             3596..4363
                     /locus_tag="VF516_22000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110428.1"
                     /translation="MTVPTGAWQRDWIRRHGGAPDARVAVRYVQTPSVFGDLRIPGDR
                     PDVAPAASFADLGDDQLAALAHQNGFAGFTTIDGANATWHHEIDFQPTDGGADIGRIE
                     PAGDGRMFEHALDDSYVESWSALDRGGGFFAARVERAGRVDQILAVAGGHFVYARARA
                     VALPPGDSLTELIARTHPPRDAVIAYLDCEISYGTTRGWQIERSTLPWQEGKRLAFVD
                     RIATSGRPAPRDPAAGEVWSFPVVELAPEVLRASFDR"
     gene            complement(4530..>5882)
                     /locus_tag="VF516_22005"
     CDS             complement(4530..>5882)
                     /locus_tag="VF516_22005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110429.1"
                     /translation="VGDAHERHADQVADRVVAGQSAVDLLAQVAPHGTSAASAPGAAS
                     VQRKPIAGNNTNQGQAYQDDRGGPTMFAYPDSLGWYWTGVYAVFHEPLADQYWLEDGR
                     CWDPVTNRVLSLVEGFWYTDGNQYYYYDGRHYIVPQDSELQSQTNGPLTTEHTEVGDL
                     QSVEISHQDAPVDLESIDVPVMSVKELREWGIKDTATARQIVIIQVDGQDWQSAWSIL
                     KGWNKHHDAFTESKTPQLQNTTSPSVETKLAGSNKRKLAPQNAPSKRPKKNEQVEPPR
                     PLTPEECGLSHEHVVKLHGERSELEDMPVFTQSVVKGNDYRTGKPMLHTAIELPTDED
                     LRVNLDRYWRGKGGEMGGGAIERKHEKHGENSDSMAEFARTAFAFARANCTRWFYQDP
                     NQIVFHNYAPDKYEPNQSVIKMAVWDYSVGGMVTFYTVEPSKVGGSIDDYMRRIGFVD
                     "
BASE COUNT          821 a         2069 c         2001 g          991 t
ORIGIN      
        1 gactttctcg ccgtgcgcga tccgggcgag catcgccccc tcctcgtcga gccgaccacc
       61 gggcaacagc ttcgcgatcg actggccgag catctccctg gcggtgtagc cgaacatgcg
      121 ctcggcgctg atgttccacg aggtgacgtc gccgccgagg gtctcgccga cgatggcgtc
      181 ctcggacgaa tcgacgatcg ccgccagccg ctccagatcg cgctcgatct tgcggcgctc
      241 ggtgatgtcg cggatcgcgg tcaagatcgt gagggcgccc gcggtccgca tcgggctcag
      301 gctgatctcg acggggaact cgctgccatc cttgcgcaga cccgcgagct cgcggcccgc
      361 tcccatcgga cgggtggacg gtgccgccgc gaatcgcgcg atgtgagcgg cgtgccgcgg
      421 tcgtgcgcgc tcggggatca gcatctcgac cgggtgaccg atcagctcgg tgcgcgcgaa
      481 tccgaacatg cgctcggccg cgacgttgac cagaaggacg gtgctctcgg catcgcagac
      541 gatcacgccc tcgggcgcga gatcgacgag atcgcgaaac cggaggtcgg cgcgttgctg
      601 ctcggccgcg atccgcgcct cgagcgccac gccctcgcga cgcgcgtgcg ccagctcggt
      661 gacatcttcg gcgcgatgga ggatgtagcg aacggcgtgg tcgccatcga gcaccggtgt
      721 gttgaccggc gaccaccacc gctgctcgaa cgtcccgtcc ggccgccgca cgtcgtactt
      781 ctgcacggcc atcgtgtcgg gctcgcgcgt cgcgatcgcg cgcgcgatcg acgcggagag
      841 gttgcgcgtg cccgtcgcct ccggatcggc cgggttgtcc ggaaacacgt cgaacaggcc
      901 acgcccgacg atcgccgcgc gttccgtcat ggtcgcgcga aggtacgcgt cgctcgcagc
      961 gaggatcgtc agcccaggat cgggcgcgag caccaggtac agaccaggtg ccgcttcgaa
     1021 cagcgcacgg aagtcgagat ccaccacccc ggccccatgt taccgctgtt cgaggctcgg
     1081 gaagaccttc tgtgcgggac cactcacgcg ccgcccgttc gatccgggct catccggacc
     1141 gccccggcgc gggtcagtcg gcgtcgatcc agaccagacg gaatccgtcc ttggtgagct
     1201 ggtcgaacgc cttctggtac tggtcgggcg tcatgccgtg gcgggcgacg aagctcggtc
     1261 caggggactt gtcccagatc gccgcgtagg ccggctcgcc gttgatctgg tatgcgctca
     1321 ccagccgcag gcggaagccc tggcggccga gctcgtcgaa cgcctgctgg tactgcggcg
     1381 cggtcatgcc atggcgcgcc acgaacgtcg ggcacggctt gcggtcccag atcgcggcgt
     1441 agcggtcctg gccggccacc gaggttccgc tgacctggga cagacaccat ccctgcttgc
     1501 cgaggctgtc gaatgcttgc tggtagtcct gcgcggtcat gccgtggcgc gcgacgaagt
     1561 ccggcccggg ggacctgtcc cagatcgcgg cgtagcgatc ctgcccggcg accgtgtagc
     1621 cgttgatcag cgtcagccgg aatccctgct tgccgaggct atcgaacgct tgctggtagt
     1681 cctgcgcggt catgccgtga cgcgcgacga tgccggcgca gcccttgcgc tcccagatcg
     1741 ccgcgaaccg cggctgaccg gcttcctcgt agccggtgac atcgaccagg cagaagccct
     1801 gctgggtctg cttgtcgaac tcctgctggt actgctggga cgtcatgccg tggcgcgcga
     1861 tccagtccgg cccggcctgc ttgacccaga tcgccgcgaa ctgcgccgga cccggtggga
     1921 tcagcgacgg actggcgcgg aacaccgcgt ggaggtacgt cgcggtgttg agctgcgcac
     1981 gcttgagcgc ctcgccgatc gccgcgtcga cggcgggctt gttgcggaag cacgggcccg
     2041 cgtagacccc gccggcgccc gagacgtaga cgcaggcgcc gcccgcgtcg ttgatcgcct
     2101 tcttgaacga accgaacagc gccttgccgc ccttggccgc cgccaccagg acgtcgtcgg
     2161 gcgagcccgc gccgggcgtc cagctggtct gcacgaagcg gtgctggatc accggcacgt
     2221 aggcctcgag cgtggggtgc aggaacagcg gcgggctcgc ccccgagtac tgcgccgcat
     2281 ggtacggcat cgtgacgtcg gtcatgtagt ggagcgccag cccgagcgcg tagcaccggc
     2341 tcttcgcgtg gtgcccgtag gtcttggccg gaaccgcgct cttgccggtc gcgaagtgct
     2401 ggatctggtt ccgcgcgttc tgccgggcgt cgaacgaccc gctgacgcag ctgctgagcg
     2461 ccagcgcgta ggtatggcaa tgatcggacg ggctcggatc gtagaagtgc gatccgtggt
     2521 tgttgctgtc gaccagctca tcgcggccgt cggcgtccca cagcccctgc cgccactcct
     2581 gggtgcacgc gtcgaggaag cgcgcggcac cgagcgcgaa cggatcgtcg ctgtgcttga
     2641 gcagatcgat cgccttctcg acgatgaaca ggtgggtatt gccgcgccac tccgccttgc
     2701 caccgacgct gtcacgcgat tccgcgtccc acgcgaaggc ggcgctcgac gcgctcgcca
     2761 cgaacaaacc gaccaagacc gctagctgtc gcttcatgac tcgatcctct gacgggcacc
     2821 gcagcccgcc cgcgcttgca cagcaagcgg gatgccggcg ccgatcgcag cgagatcaaa
     2881 ggcgctgccc gatcgcggcc caggcacaag cgcggcacgt gccacggccg gtgccaggaa
     2941 cgcatcgtgg acctcagcga tgcgagaagc ggtcgccgcc gcgacacgtg gacgcaccgg
     3001 atacctgcgt cgtgcaaccc cgtctgcccg ccgtgacgct gcggtggtac acccgcggtc
     3061 atggcggcgc ggaaatcgct gatgatcttg atcgcgggcc cctatcgctc cggcaccggc
     3121 gatgacccgg gcaagctggc ggcccatgtc gcggcgatgg aggcgctggc gctgccggtg
     3181 ttccgcgcgg gccacgtacc ggtggtcggc gagtggctcg cgctgccgct gtgccggctc
     3241 gccggctcca cccaggtcgg tgacgctgcg tttcacgagg tgttccaccc gatcgccgag
     3301 cggctgctcg agcactgcga cggcgtgctg cgggtcggcg gcgcctcgca gggggcggac
     3361 ctgatggtcg cgaccgccga gcggctcggg ctcgcggtct accgccgcct ggaggacatc
     3421 cccggctgca gcgccacctg accggcgcct cgccgcgcgc ggcatgtcat ggccccatcc
     3481 ggggtataga gcaccgatgc gacctcgtgg tctatccttc ctgtgccatg tcgccacgtt
     3541 cgcgcccatc ctcgcctgct caccaccggc cgcgccgcac ccgtcggtcg ctgccgtgac
     3601 cgtcccgacc ggcgcctggc aacgcgactg gatccgccgg cacggcggtg cgccggacgc
     3661 ccgcgtcgcc gtgcgctacg tccagacccc gagcgtgttc ggcgacctgc gcatccccgg
     3721 cgatcggccg gacgtcgcgc cggccgcgtc gttcgccgac ctcggggacg accagctggc
     3781 cgcgctggcc caccagaacg gctttgccgg cttcaccacg atcgacggcg cgaacgcgac
     3841 ctggcaccac gagatcgact ttcagcccac ggacggcggc gccgacatcg gtcgcatcga
     3901 gccggccggc gacgggcgga tgttcgagca tgcgctcgac gactcgtacg tcgagagctg
     3961 gtccgcgctc gatcgcggcg gcgggttctt cgccgcgcgc gtcgagcgag cgggtcgcgt
     4021 cgaccagatc ctcgccgtcg ccggcgggca cttcgtctac gcccgtgcgc gcgccgtggc
     4081 cctgccgccc ggcgactcgc tcaccgagct gatcgccagg acccatccgc cgcgcgacgc
     4141 cgtcatcgcg tacctcgact gcgagatctc gtacggcacc acccgcggct ggcagatcga
     4201 gcgctcgacg ctgccatggc aagagggcaa gcggctcgcg ttcgtcgacc ggatcgcgac
     4261 cagcggccgt cccgcgccgc gcgaccccgc cgcgggcgag gtctggagct tcccggtcgt
     4321 cgagctcgcg cccgaggtgc tccgggcaag cttcgatcga tgacggtgag ccggaaaccg
     4381 cgccgccagc gagctcgcca gcttgccgcc gcctgccggc aggatgacct tgcacgcatc
     4441 gtccgcggcg cgtgatcctc gggatcgggg gaggtccacg atgacgcagc ggacgcggcc
     4501 cggcctgggc atcctgcccg cgcgcccggt cagtccacaa agcctatgcg gcgcatgtaa
     4561 tcgtcgatgc tgcctcccac cttgctcggc tcgaccgtgt agaacgtgac catcccccct
     4621 acgctgtagt cccagaccgc catcttgatc acgctctggt tgggctcgta tttgtctggg
     4681 gcgtagttgt ggaagacgat ctgattgggg tcctgataga accatcgcgt gcagtttgct
     4741 ctcgcgaagg caaacgccgt cctggcgaac tccgccatcg agtcactgtt ctcgccgtgc
     4801 ttctcgtgct tgcgctcgat cgcgccgccg cccatttcgc cgcccttacc gcgccagtag
     4861 cggtcgaggt tgacccgcag atcctcgtcg gtgggtaact cgatcgcggt atgcagcatc
     4921 ggcttgcctg tccggtagtc gttgcctttc accacgctct gcgtgaagac cggcatgtcc
     4981 tcgagctcgg agcgctcgcc gtggagcttg acgacgtgct cgtggctcag cccgcactcc
     5041 tcgggagtga gcggcctcgg aggttcgacc tgctcgttct tcttcgggcg cttggacggg
     5101 gcgttctggg gcgccagctt cctcttgttg gacccggcga gcttggtctc gacggagggc
     5161 gaggtcgtgt tctggagttg cggggtcttg ctctcggtga acgcatcgtg gtgcttgttc
     5221 cagcccttga ggatcgacca cgcggactgc cagtcctggc catcgacctg gatgatgacg
     5281 atctgcctgg cggtggcggt gtccttgatc ccccattcgc gcagctcctt cacgctcatg
     5341 acggggacgt cgatcgactc gagatcaacg ggggcgtcct ggtgcgagat ctcgacggac
     5401 tgcaagtcgc cgacctcggt gtgctccgtc gtgagcggtc cattggtttg tgattgcagc
     5461 tcgctgtctt gcgggacgat gtagtggcgg ccatcgtagt agtaatactg gttgccgtcc
     5521 gtataccaga acccctcaac gaggctgagc acccggttgg tgaccggatc ccagcaccgg
     5581 ccatcttcca gccagtattg atcggccaac ggttcgtgaa agaccgcgta cacacctgtc
     5641 cagtaccagc ccagggagtc cgggtacgcg aacatcgtcg ggccgccccg atcatcctgg
     5701 tacgcctggc cctggttcgt gttgttgcct gcgatcggct tgcgctggac tgacgcggcg
     5761 ccgggcgccg atgcggcgct ggtcccgtgc ggcgcgacct gggccaggag gtccaccgcc
     5821 gactgcccgg cgaccacgcg gtcggccacc tggtcggcgt ggcgctcgtg ggcgtcgccc
     5881 ac
//
LOCUS       DASVOR010001152         1659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_449870,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001152 DASVOR010000000
VERSION     DASVOR010001152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1659
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449870"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF516_22010"
     CDS             <1..237
                     /locus_tag="VF516_22010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110430.1"
                     /translation="AIVVATGGSPTSVEPPAAPVPSVQVSEALGQIRGLADRISHAVT
                     TTRTPLDTELDNLIRDGKRGLAAVLETGGLDGLP"
     gene            complement(250..>1659)
                     /locus_tag="VF516_22015"
     CDS             complement(250..>1659)
                     /locus_tag="VF516_22015"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8110431.1"
                     /translation="LLVGILTAHYGVEGSSFYGIPLAKILPYSVVRTWHIQLGIFWIA
                     TAWLAAGLAIAPSVGKEPRRQKLLVNVLFGALVVVVLGSLAGELLAVQGKLGADAVWW
                     FGHSGYEYVDLGRCFQIALLIGLILWVVLVGRALWPALRRRDAQRPLLMMLLISTIAI
                     GGFYFAALGAGRHTNLAIAEYWRWWVVHLWVEGFFEVFATVIIAFLFVRLGLVRAASA
                     GRASVLAATIFLGGGVIGTLHHLYFSGTPTIALAFGAVFSALEVVPLMFLGYDAWENW
                     RHTRSTDWMRAYRWPIYFFIAVAFWNLVGAGLFGFMINPPIALYYMQGLNTTPVHGHT
                     ALFGVYGMLGMGLVLFTVKSLRPDLVWPDRLLGAAFWSINLGLGAMVLLSVLPVGLLQ
                     TWAAVEHGYWYARSAEFLHTPVMDALRWMRVVGDTVFAAGAIAFVVAVARLTLGRARS
                     AGPKIEGDRDADIDAAISV"
BASE COUNT          295 a          591 c          592 g          181 t
ORIGIN      
        1 gcgatcgtgg tcgcgaccgg cggttcgccc acctcggtcg agccgcccgc cgcgccggtg
       61 ccgtcggtcc aggtgtccga ggcgctgggc cagatccgcg ggctcgccga ccggatctcg
      121 cacgcggtca ccaccacccg gacgccgctc gacaccgagc tcgacaacct gatccgcgac
      181 ggcaagcgcg ggctcgccgc ggtgctggag accggcggcc tcgacggatt gccgtagccg
      241 ccgccgcgct cacaccgaga tcgccgcgtc gatgtcggcg tcgcggtcgc cttcgatctt
      301 cggcccggcg ctgcgcgcgc ggccgagcgt gagccgggcg accgcgacga cgaacgcgat
      361 cgcgccggcg gcgaacacgg tgtcgccgac cacgcgcatc cagcgcagcg cgtccatcac
      421 cggggtgtgg aggaactcgg cgctgcgcgc gtaccagtag ccgtgctcga cggcggccca
      481 ggtctggagc aggccgaccg gcagcacgct gagcagcacc atcgcgccga ggccgaggtt
      541 gatcgaccag aacgccgcgc cgagcaggcg atcgggccac accaggtcgg gccgcagcga
      601 cttgacggtg aacagcacca gccccatgcc gagcatgccg tacacgccga acagcgcggt
      661 gtggccgtgg accggcgtgg tgttgaggcc ctgcatgtaa tacagcgcga tcggcgggtt
      721 gatcatgaag ccgaacaggc cggcgccgac caggttccag aacgcgacgg cgatgaagaa
      781 gtagatcggc cagcggtagg cgcgcatcca gtcggtcgac cgggtgtggc gccagttctc
      841 ccacgcgtcg tagcccagga acatcagcgg cacgacctcg agcgcgctga acaccgcgcc
      901 gaacgccagg gcgatcgtcg gcgtgccgct gaagtacagg tggtgcagcg tgccgatcac
      961 gccgccgccg aggaagatcg tcgccgccag cacggaggcg cggccggccg acgcggcgcg
     1021 gaccaggccg aggcggacga acaggaacgc gatgatcacg gtcgcgaaca cctcgaagaa
     1081 gccctcgacc cacaggtgga cgacccacca gcgccagtac tcggcgatcg ccaggttggt
     1141 gtggcggccg gcgccgagcg cggcgaagta gaacccgccg atcgcgatcg tcgagatcag
     1201 cagcatcatc agcagcggac gctgcgcgtc gcggcggcgc agcgccggcc acagcgcgcg
     1261 gccgaccagc acgacccaca ggatcaggcc gatcagcagc gcgatctgga agcaccggcc
     1321 gaggtcgacg tactcgtacc cggagtggcc gaaccaccac acggcgtcgg cgccgagctt
     1381 gccctgcacc gcgagcagct cgccggccag cgagccgagc acgaccacga ccagcgcacc
     1441 gaacagcacg ttgaccagca gcttctggcg gcgcggctcc ttgccgaccg acggcgcgat
     1501 cgccaggccg gcggcgagcc acgcggtcgc gatccagaag atgccgagct ggatgtgcca
     1561 ggtccggacc accgagtacg gcaggatctt cgcgagcggg atgccgtaga acgacgagcc
     1621 ctcgacgccg tagtgcgcgg tgaggatgcc gaccaggag
//
LOCUS       DASVOR010001153         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420135,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001153 DASVOR010000000
VERSION     DASVOR010001153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420135"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..205
                     /locus_tag="VF516_22020"
     CDS             <1..205
                     /locus_tag="VF516_22020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110432.1"
                     /translation="LVQARTLLVRAYALDPRPATLLELAAVDLQTGRCREAQRAVQRV
                     IADGVGPLSSQAWELMGRIGRCD"
     gene            372..1763
                     /gene="zwf"
                     /locus_tag="VF516_22025"
     CDS             372..1763
                     /gene="zwf"
                     /locus_tag="VF516_22025"
                     /inference="COORDINATES: protein motif:HMM:NF009492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEX8110433.1"
                     /translation="MRQPQSDALVLFGATGDLAYKQIFPALQAMAKHGTLNVPVVCVA
                     RERRSVESLRERVRDSLENHGGIDRGAFDKLAGLLRYVAIDYNDPQTFSELKQALGGA
                     QRPLHYLAIPPSAFATAIQGLHQAGCTANARIALEKPFGRDLASARMLDRLLHTAFPE
                     SAIFRIDHYLGKEPVQNMLYFRFANSVLEPLWNRDHVSRVEITMAEQFGVEGRGRFYE
                     ETGAIRDVVQNHLLQVTAILAMDAPVGQNVEAIRDEKARVLKAIEPLDPEHVIRGQYR
                     GYRSEPGVAPNSTVETFAAVELRIDTWRWADVPFTIRAGKRLATTLTEVVAEFKNPPR
                     DIFREHLICPNYVRFRVGPDTAIALGVRVLRPRAAPGEVLGRDNELIATQDPALGALP
                     YERLLDEALRGDQTLFARQDEVEAQWRVVDPILGDVVPVHLYEPGSWGPIEAEPVAPC
                     GCHSVVTTVRKAS"
     gene            1760..>2059
                     /locus_tag="VF516_22030"
     CDS             1760..>2059
                     /locus_tag="VF516_22030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110434.1"
                     /translation="MSEPASLGLPPRIAACLFDLDGVLTRTAILPAAAWKQAFDPLLV
                     ARGQPAFDAVADYTRYVDGKPREDGVRSLLAARGIALPDADVRALAARKDQLFAHG"
BASE COUNT          293 a          757 c          729 g          280 t
ORIGIN      
        1 gctcgtgcag gcgcgcacgc tgttggtccg ggcgtacgcg ctcgatcccc ggccggccac
       61 gctgctcgag ctcgcggcgg tggatctcca gaccggccgc tgccgcgagg cccagcgcgc
      121 cgtccagcgg gtgatcgccg atggggtcgg cccgctgtcg tcccaggcct gggagctgat
      181 gggcaggatc ggccggtgcg actgagcggc agcgctccgg atcgcccgat ctccgccgct
      241 ggctgacagc gatcggcgcg atcggccgcg cgcgcggatg gtcagtgccg gcacagcgat
      301 cgcaagcgct gtgggggtgc tcgacgagcg catgctcgcc gagccggcca ggcacaacga
      361 aggagacgga gatgcggcaa cctcagtccg acgcgctggt cctgttcggc gccacgggcg
      421 acctcgcgta caagcagatc ttcccggcgc tgcaggcgat ggcgaagcac ggcacgctga
      481 acgtgccggt cgtctgcgtg gcgcgcgagc gtcgcagcgt cgaaagcctg cgcgagcgcg
      541 tgcgcgacag cctcgagaac cacggaggca tcgatcgcgg cgcgttcgac aagctcgcgg
      601 ggctgctgcg ctacgtcgcg atcgactaca acgaccccca gacgttctcc gagctcaagc
      661 aggcgctcgg cggcgcgcag cggccgctgc actacctcgc gatcccgccg agcgcgttcg
      721 cgaccgcgat ccagggcctg caccaggccg ggtgcaccgc gaacgcgcgg atcgcgctcg
      781 agaagccatt cgggcgcgat ctcgcgtcgg cgcgcatgct cgaccggctg ctccacaccg
      841 cgttccccga gtcggcgatc ttccgcatcg atcactatct cggcaaggag ccggtccaga
      901 acatgctgta cttccggttc gccaactcgg tgctcgagcc gctgtggaac cgcgaccacg
      961 tcagccgcgt cgagatcacg atggcggagc agttcggcgt cgagggccgc ggccggttct
     1021 acgaggagac cggcgcgatc cgcgacgtcg tgcagaacca cctgctccag gtcaccgcga
     1081 tcctcgcgat ggacgcgccg gtcgggcaga acgtcgaggc gatccgcgac gagaaggcgc
     1141 gcgtgctcaa ggcgatcgag ccgctcgatc ccgagcacgt catccgcggc cagtaccgcg
     1201 gctatcgcag cgagcccggc gtcgcgccga actcgacggt cgagaccttc gccgccgtcg
     1261 agctgcgcat cgacacctgg cggtgggccg acgtgccgtt caccatccgc gccggcaagc
     1321 gcctggcgac cacgctcacc gaggtggtcg cggagttcaa gaatccgccg cgcgacatct
     1381 tccgcgagca cctgatttgc ccgaactacg tgcggttccg cgtcggcccc gacaccgcga
     1441 tcgcgctcgg ggtccgcgtg cttcgcccgc gcgccgcacc cggcgaggtg ctcggccgcg
     1501 acaacgagct gatcgcgacc caggaccccg cgctcggggc gctgccgtac gagcggctgc
     1561 tcgacgaggc gctgcgcggc gaccagacgc tgttcgcgcg ccaggacgag gtcgaggccc
     1621 agtggcgcgt ggtcgacccc atcctcggcg acgtcgtgcc ggtccacctc tacgagccgg
     1681 gctcgtgggg gccgatcgag gccgagccgg tcgcgccgtg cggctgccac agcgtggtca
     1741 ccaccgtgag gaaagcctcg tgagcgagcc cgcgtcgctc gggctgccgc cgcgcatcgc
     1801 ggcctgcctg ttcgacctcg acggcgtgtt gacccggacc gcgatcctcc ccgccgcggc
     1861 gtggaagcag gcgttcgacc cgctgctggt cgcgcgcggc cagcccgcgt tcgacgcggt
     1921 cgccgattac acccggtacg tcgacggcaa accgcgcgag gacggcgtgc gctcgttgct
     1981 cgccgcgcgc ggcatcgcgc tcccggacgc cgacgtgcgc gcgctcgccg cccgcaagga
     2041 tcagctgttc gcgcacggg
//
LOCUS       DASVOR010001154         3810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420137,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001154 DASVOR010000000
VERSION     DASVOR010001154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420137"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1636
                     /locus_tag="VF516_22035"
     CDS             <1..1636
                     /locus_tag="VF516_22035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110435.1"
                     /translation="VGPAARPADAAENKVLVDLGLEFDPQKADGEIKALRAQLEKASA
                     NYEREVADGKRVRAESQAMRERTDDLKAQIKDREDQIGARDRVADELRDELQQTRNEL
                     NKARGELGDMAENVAARERQAARAVEDTAKLREDLEDKNRQLMELSRTKDEGWKKLNE
                     QLTEIEHLREVINEQERMLEERRVGLISQEEVIKELRAEKEKNLKLIAQLRAERDEAN
                     TNASRNAAQINGLEEENRRLGRLLVEAQTDAGRGGGAQPDQLMKMTGDIRELRVELKK
                     VEADRDRLDEQFKLADRDREKLEGRLAQIEVELQEAVHARLAAESARNVAQDALAKAE
                     VARHKAAEEALNASKARDAASTGGDDARRELDRLRRKLAELEKVQAAAPAAPAIDPKE
                     LQLEREATERKLKDALDKAANAERSLKSLQAEVDAAKTDAAKARAEAARAKAAADAQA
                     VSVLDNAATTISAPPVAGNGAEIARLAQEVYESINDILSEMRNNMRLVQGELPNLKAD
                     GGTLQAVTDAVEALVDNAETAKGALRGLRDLANANG"
     gene            1658..3274
                     /locus_tag="VF516_22040"
     CDS             1658..3274
                     /locus_tag="VF516_22040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8110436.1"
                     /translation="MTIEHKRSSKGEIEDLIRARYSLIWITSPEENRVEEALKRLCVE
                     REMRLEVWSITEGFKTVANGQGTRDVKDPMKAIDHVLRAEGRALFVLRDFHPFLKEPA
                     VVRRLRDAAHELRKTKKSLLVLAPVTKVPPELEKSISAVLDWDLPNRAEIEAAARKVL
                     PSLPAAAQQQLESDSQLMERVVEGALGLTLVEAENVFAKSAVRSHTFDLETILEEKKQ
                     IIRKSGLLEYYEHREEFSDVGGMEVLKDWLVKRRNAFGTRARDFGLPLPKGILLIGVP
                     GTGKSLTAKAVGALWQMPLLRLDVGKIFAGLVGSSEENIRSVIKTAEAIAPAILWIDE
                     LEKGFSGTASSGQTDGGTTSRVFGTFITWLQEKTTPVFVIATANNVHQLPPELLRKGR
                     FDEIFFCDLPDREDRQQILDIHIRKKNRDPGQFDMDKLIEATVDFSGAELEQAVVAAL
                     YDAFDTGNDLTTEGLLHTLKDMVPLAITMREMIESMREWARTRARMASARNRAPAKKE
                     GWMSRYGAPRASLGDKAPETSDDGERKLEL"
     gene            3304..3711
                     /locus_tag="VF516_22045"
     CDS             3304..3711
                     /locus_tag="VF516_22045"
                     /inference="COORDINATES: protein motif:HMM:NF018565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1257 domain-containing protein"
                     /protein_id="HEX8110437.1"
                     /translation="MSHFTKCALKMTNLAAIKKALADMELKFTEAEQGQTVSVRGYRG
                     DKLQAAISVDMGRYDVGVIDNQDGTYDITADWWGVETTKGVSEEEFKHQLSQRYQYHN
                     VKQACEDKGYSVEEEVNEEDGSIRLVVRKWVSE"
BASE COUNT          756 a         1262 c         1363 g          429 t
ORIGIN      
        1 ggtcggtccg gcggcgcgcc cggcggacgc cgccgagaac aaggtgctgg tcgacctcgg
       61 gctcgagttc gatccgcaga aggccgacgg cgagatcaag gcgctgcgcg cccagctcga
      121 gaaggcgtcg gccaactacg agcgcgaggt cgccgacggc aagcgcgtgc gcgccgagtc
      181 ccaggcgatg cgcgagcgga ccgacgacct caaggcgcag atcaaggacc gcgaggacca
      241 gatcggggcg cgcgaccggg tcgccgacga gctgcgcgac gagctgcagc agacccgcaa
      301 cgagctcaac aaggcgcgcg gcgagctcgg cgacatggcc gagaacgtcg ccgcccgcga
      361 gcgccaggcc gcgcgcgcgg tcgaggacac cgccaagctg cgcgaggacc tcgaggacaa
      421 gaaccgccag ctgatggagc tgtcgcggac caaggacgag ggctggaaga agctcaacga
      481 gcagctgacc gagatcgagc acctgcgcga ggtgatcaac gagcaggagc gcatgctcga
      541 ggagcgccgg gtcggcctga tctcgcagga ggaagtgatc aaggagctgc gggccgagaa
      601 ggagaagaac ctcaagttga tcgcccagct ccgcgccgag cgcgacgagg ccaacacgaa
      661 cgcgtcgcgc aacgccgccc agatcaacgg cctcgaggaa gagaatcgcc ggctcggccg
      721 gctcctggtc gaggcccaga ccgacgccgg gcgcgggggc ggggcccagc ccgatcagct
      781 gatgaagatg accggcgaca tccgcgagct gcgggtcgag ctcaagaagg tcgaggccga
      841 ccgcgaccgg ctcgacgagc agttcaagct cgccgaccgc gatcgcgaga agctggaggg
      901 ccggctcgcg cagatcgagg tcgagctcca ggaggcggtc cacgcccggc tcgccgccga
      961 gagcgcgcgc aacgtcgcgc aggacgcgct cgccaaggcc gaggtcgcgc gccacaaggc
     1021 cgccgaggag gcactcaacg cgtccaaggc gcgcgacgcc gcgtcgaccg gcggcgacga
     1081 cgcgcgccgc gagctcgatc gcctgcgcag gaagctcgcc gagctcgaga aggtgcaggc
     1141 cgcggcaccg gccgcaccgg cgatcgaccc caaggagctc cagctcgagc gcgaggccac
     1201 cgagcgcaag ctcaaggacg cgctcgacaa ggcggccaac gcggagcgct cgctcaagtc
     1261 gctgcaggcc gaggtcgacg ccgccaagac cgacgccgcc aaggcccgcg ccgaggccgc
     1321 acgcgccaag gccgcggccg acgcccaggc ggtgtcggtg ctcgacaacg cggccaccac
     1381 gatcagcgcg ccccccgtgg ccggcaacgg cgccgagatc gcccggctcg cccaggaggt
     1441 ctacgagtcg atcaacgaca tcctgtcgga gatgcgcaac aacatgcggc tggtgcaggg
     1501 cgagctgccc aacctcaagg ccgacggcgg aacgctccag gcggtgaccg acgcggtcga
     1561 ggcgctggtc gacaacgccg agaccgccaa gggcgcgctg cgcgggctgc gcgatctcgc
     1621 caacgcgaac gggtgaccga atggacgacg tacagccatg acgatcgagc acaaacgaag
     1681 cagcaagggc gagatcgagg acctgatccg cgcgcgctac tccctgatct ggatcacctc
     1741 gcccgaggag aaccgcgtcg aggaagcgct caagcggctg tgcgtcgagc gcgagatgcg
     1801 gctcgaggtg tggtcgatca ccgagggatt caagacggtc gccaacggcc agggcacgcg
     1861 cgacgtcaag gatccgatga aggcgatcga tcacgtgctg cgcgccgagg gccgcgcgct
     1921 gttcgtgctc cgcgacttcc acccgttcct caaggagccc gcggtggtgc gccggctgcg
     1981 cgacgccgcg cacgagctgc gcaagaccaa gaagagcctg ctggtgctgg cgccggtcac
     2041 caaggtgccg cccgagctcg agaagagcat ctcggcggtg ctcgactggg acctgcccaa
     2101 ccgcgccgag atcgaggccg cggcgcgcaa ggtgttgccg agcctgcccg cggcggccca
     2161 gcagcagctc gagagcgact cgcagctcat ggagcgcgtc gtcgagggcg cgctcgggct
     2221 gaccctggtc gaggccgaga acgtgttcgc caagtcggcg gtgcgcagtc acacgttcga
     2281 cctggagacc atcctcgagg agaagaagca gatcatccgc aagagcggcc tgctcgagta
     2341 ctacgagcac cgcgaggagt tctccgacgt cggcggcatg gaggtgctca aggactggct
     2401 ggtcaagcgg cgcaacgcgt tcggcacccg cgctcgcgac ttcggcctgc cgctgcccaa
     2461 gggcatcctc ctgatcggcg tgcccgggac gggcaagtcg ctgaccgcca aggcggtcgg
     2521 cgcgctgtgg cagatgccgc tgctccggct cgacgtcggc aagatcttcg ccgggctggt
     2581 cggcagctcc gaggagaaca tccgctcggt gatcaagacc gccgaggcga tcgcgccggc
     2641 gatcctgtgg atcgacgagc tggagaaggg gttctcgggc accgcgtcgt cgggccagac
     2701 cgacggcggc accacgtcgc gcgtgttcgg cacgttcatc acctggctgc aggagaagac
     2761 caccccggtg ttcgtgatcg cgaccgcgaa caacgtccac cagctgccgc ccgagctgct
     2821 ccgcaagggc cggttcgacg agatcttctt ctgcgatctg ccggatcgcg aggaccgcca
     2881 gcagatcctc gacattcaca tccgcaagaa gaaccgcgac ccggggcagt tcgacatgga
     2941 caagctgatc gaggccacgg tcgacttctc gggcgccgag ctcgagcagg cggtggtcgc
     3001 cgcgctgtac gacgcgttcg acaccggcaa cgacctcacg accgagggcc tgctccacac
     3061 gctcaaggac atggtcccgc tcgcgatcac gatgcgggag atgatcgagt cgatgcgcga
     3121 gtgggcgcgg acccgggcgc gcatggcgtc cgcccgcaac cgcgcacctg ccaagaagga
     3181 ggggtggatg tcgcgctacg gggcgccgcg cgccagcctg ggtgacaaag cgccggagac
     3241 cagcgacgac ggggaacgca agctggagct gtgacgtaga ctggacagga agaaagcgaa
     3301 ccgatgtccc acttcacgaa gtgcgccctg aagatgacca acctggcggc gatcaagaag
     3361 gcgctcgccg acatggagct caagttcacc gaggccgagc aggggcagac ggtgtcggtc
     3421 cgcggctacc gcggcgacaa gctgcaggcc gcgatctcgg tcgacatggg ccggtacgac
     3481 gtcggcgtca tcgacaacca ggacgggacc tacgacatca ccgcagactg gtggggggtc
     3541 gagaccacga agggggtctc cgaggaagag ttcaagcacc agctcagcca gcgctaccag
     3601 taccacaacg tcaagcaggc ctgcgaagac aagggctact cggtcgagga agaggtgaac
     3661 gaggaggacg gatcgatccg cctggtcgtt cgcaagtggg tctcggagta gcgaggccag
     3721 gaggccacca tgcacaagca agacatcgag atcgtgatca acgccaaggg cgaggtgacg
     3781 ttccaggtca agggcgtcaa gggcggcagc
//
LOCUS       DASVOR010001155         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_245578,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001155 DASVOR010000000
VERSION     DASVOR010001155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245578"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VF516_22050"
     CDS             complement(<1..792)
                     /locus_tag="VF516_22050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110438.1"
                     /translation="MLSVGSILGGKFQLQRLAGTGGMGEVFEARCLDSGQRVAVKMLR
                     ARQADQLVRFEREARLLAEFDHPNIVRYIEHAVTPLGQPYLVMEWLDGEDLATRLRTR
                     PLTLHETVAIGRDVAEAMAAAHERGIVHRDLKPGNVFLVRGAFLARGEFSQSKVLDFG
                     IAWLSDWTPVTQAGATPGTVGYMAPEQIRHGAELTPAADVFALGCLLFEGLTGRPAFE
                     RSETGTAMAADRRVAPAAPLGSRLGGEVPAELVALIEQMLAREPDQ"
     gene            1121..1870
                     /locus_tag="VF516_22055"
     CDS             1121..1870
                     /locus_tag="VF516_22055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8110439.1"
                     /translation="MSRPLALLIHSGGFTSRQWRRLAQRLAPTHDVLAPDLIGYGDEP
                     WPPGKPFHFQRDVERLAGMLDRPADVVGHSYGGLLGLQLALARPERVRSLALYEPVAF
                     GVLGPADADARAQLALPAYPADAAGVDERWLEAFVDWWNGAGAWAALAEPTRAGFRAV
                     AWKVYQEVASLSADTTDHARYATITAPTLLLGGGRTPLTERRTLERLAEALPHARLEV
                     FPDMGHMGPITHADAVGEAIAAHVARFAAAS"
BASE COUNT          317 a          813 c          778 g          312 t
ORIGIN      
        1 ctggtccggc tcgcgcgcga gcatctgctc gatcagcgcc accagctccg ccgggacctc
       61 gccgccgagc cggctgccca gcggcgccgc cggggcgacc ctccggtcgg cggccatcgc
      121 cgtgccggtc tcgctgcgct cgaacgccgg ccgcccggtc aggccctcga acagcaagca
      181 gccgagcgcg aacacgtcgg cggccggggt gagctctgcg ccgtggcgga tctgctcggg
      241 cgccatgtag cccaccgtcc cgggcgtcgc gccggcctgg gtgaccggcg tccagtccga
      301 cagccaggcg atcccgaagt cgagcacctt gctctggctg aactcgccgc gcgcgaggaa
      361 cgcgccgcgc accaggaaca cgttgccggg cttgaggtcg cggtggacga tgccgcgctc
      421 gtgcgccgcc gccatcgcct cggcgacgtc ccggccgatc gcgaccgtct cgtgcagggt
      481 cagcggccgg gtgcgcaggc gggtcgcgag gtcctcaccg tcgagccact ccatcacgag
      541 gtacggctgc ccgagcggcg tgaccgcgtg ctcgatgtag cgaacgatgt tggggtggtc
      601 gaactcggcg agcaggcgcg cctcacgctc gaaccgcacc agctggtcgg cctgccgcgc
      661 gcgcagcatc ttgaccgcca cccgctggcc ggaatccagg caccgggcct cgaacacctc
      721 gcccataccg ccggtgcccg ccagccgctg gagctggaac ttgccgccga gaatgctgcc
      781 gactgatagc acgagaattc agctaactag aatttatgcc gccaatgtgg attcgctgaa
      841 cgtcaagtga ccgaatacag tgatctgttc cagatactca ccctactgcg cttgcagcta
      901 ggttggatcc ttcgagggtc gaaaattcgc ccgagccgcg atcggaaatc cgcgtacgca
      961 aactctgcgt ggtgatctga ccatgcccgc agcaatttgc tgcgttgttc cgtatgcgca
     1021 gtcggaggct ggttgccgct ggtcggacag cgcctcacgt gaaccgacgc tccgggggcg
     1081 gtccgatcgg cgccccggcg gcgaggtagg ctcgcttgcc gtgtccaggc cgctcgcgct
     1141 cctcatccac agcggaggct tcacgtcgcg ccagtggcgc cggctcgccc agcgcctcgc
     1201 gccgacccat gacgtgctcg cgcccgatct gatcggctac ggcgacgagc cgtggccgcc
     1261 gggcaagccg ttccatttcc agcgggacgt cgagcggctc gccggcatgc tcgaccggcc
     1321 cgcggacgtg gtcggccact cctacggcgg gctgctcggg ctgcagctcg cgctcgcccg
     1381 acccgagcgg gtgcgatcgc tggcgctgta cgagccggtc gcgttcggcg tgctcggccc
     1441 ggcggatgcc gatgcccgcg cccagctcgc gctgcccgcg taccctgcgg acgccgccgg
     1501 ggtcgacgag cgctggctgg aggcgttcgt cgactggtgg aacggcgccg gtgcgtgggc
     1561 cgcgctcgcc gaaccgaccc gggcggggtt tcgcgcggtc gcgtggaagg tctaccagga
     1621 ggtcgcgtcg ctgtcggccg acacgaccga ccacgcgcgc tacgcgacca tcaccgcgcc
     1681 gacgctgctg ctcggcggcg gccgcacccc gctcaccgag cgccggacgc tggagcggct
     1741 cgccgaggcg ctgccgcacg cccggctcga ggtgtttccg gacatggggc acatggggcc
     1801 gatcacgcac gccgacgcgg tcggtgaggc gatcgccgcg cacgtggcgc ggttcgccgc
     1861 ggcgagctga ggatccgtat cgcgcgccac gccgggcctc gccggtgatg acagccgtgt
     1921 gcaacctgcg cgcggtggga cccgagtcac caggttcacg agcagcttcc gccggttatc
     1981 cgaggaagat gcgcggatgc ggctcgtaac aggcgaggca accacggcgc cgaggagagc
     2041 ctcatgagcc tgagcccgag cccagaacga gcccacgcac agaccggatc gcatggcccg
     2101 gctgccaccg ccgcgacacc cgggaaacgc acgcaggtcg aactggcgtc ctcggccggt
     2161 catcacggcg ccggcccgca tgccgaggct gcgagcgcgg cgagcccgtc cggcatcaac
//
LOCUS       DASVOR010001156         3031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362460,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001156 DASVOR010000000
VERSION     DASVOR010001156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3031
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362460"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="VF516_22060"
     CDS             <1..1028
                     /locus_tag="VF516_22060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110440.1"
                     /translation="TGSGAQPARPDQPAGTSPASTDDFVRNQIAALRKFSHDKESDPA
                     FAEHEGPDHACRVDHDQGIVTLTFNADGTLHCKLAEVAEGYELDVWVLTMKGVYDTGN
                     HYRVTVATGAALSFAPIRGTSADVKAEIAVLSGLHAEATEAAWWHARSHYGPYHFDSA
                     TITIALDECAVSQDTKLTIVPLYRFALTVLVLAGPGVPGHSIADGKIAESRNRADLAY
                     YFGIHLFPFSWHRDGKKHTLPGRYFSADYSDWYDRISVVAGADIAHPTEAGYIGGAVE
                     VYGGVSITAGWQPRKYNRLQSGYAVGDMFMGDQVPTDAVWKLDGWAVGISIDASLLKT
                     LLGYIGR"
     gene            complement(1051..2109)
                     /locus_tag="VF516_22065"
     CDS             complement(1051..2109)
                     /locus_tag="VF516_22065"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8110441.1"
                     /translation="MRDTRGRIPGLDGLRAISILIVLCAHSAGTRNVPSWLQVSVFGD
                     LGVRTFFVISGFLITTLLLAEYRKRGRISLRDFYIRRVYRIFPAFYVYLAILLTITAL
                     GWIELPAHDVAFAATYTMNFHAERTWWLGHLWSLAVEEQFYLLWPFALSVLRPQRALW
                     LAIGAIIAAPILRVTAFKLWPEIGPLTDQAFPFVFDSLATGCVLALGREWLESSPTYL
                     MWIDAPGWAFWTLPLACVYALSIHRAGFNLAFGVTLGNLGIAMAIHRCVRHLTLPVGR
                     VLESRPLVFIGALSYSLYLWQQPFVNRHADSIVNVFPFHLAFALGAAMLSYYLVEQPM
                     LALRARRAARKQAAARAE"
     gene            complement(2200..>3031)
                     /locus_tag="VF516_22070"
     CDS             complement(2200..>3031)
                     /locus_tag="VF516_22070"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8110442.1"
                     /translation="DYTTAARVAASFAWRQGARRMAFFYCSTSAFCTDPVDGAKTFLQ
                     ALGGTRIGRDLFVELADDAPAIERKVLRFFHDELRYKAAHPDYEIADWVWFGNTSPNA
                     ASLGKALHKVKTQLGIDVHVIGNNWSVDESLYGRCGDACVGFHVVQPFALFGDVTAAG
                     SAGLVADHARYRAIDGDPPDAYKTVQYVYGRVAVATWKLAVERLLDQGKPVTGDNLRD
                     TMESFHSVDIEGFATIGYTATDHRPQSGARITRLGPRGRMETIGQPLALSLQSGWLGW
                     "
BASE COUNT          512 a         1058 c         1071 g          390 t
ORIGIN      
        1 cgaccgggtc gggcgcccag cccgcccggc ccgaccagcc ggcggggacc agcccggcgt
       61 cgaccgacga cttcgtgcgc aaccagatcg ccgcgctgag gaagttctcc cacgacaagg
      121 agagcgatcc ggcgttcgcc gagcacgagg gccccgatca tgcgtgccgc gtcgatcacg
      181 accaggggat cgtgacgctg acgttcaacg ccgacggcac gctgcactgc aagctcgccg
      241 aggtcgccga gggctacgag ctcgacgtct gggtcctgac catgaagggc gtgtacgaca
      301 ccggcaacca ctaccgggtc accgtcgcga ccggcgcggc gctcagcttc gcgccgatcc
      361 gcggcacgtc cgcggacgtc aaggccgaga tcgcggtgct gagcgggctg cacgccgagg
      421 ccaccgaggc ggcgtggtgg cacgccagga gccactacgg cccgtaccac ttcgacagcg
      481 cgacgatcac gatcgcgctc gacgagtgcg cggtatcgca ggacaccaag ctgacgatcg
      541 tgccgctcta ccggttcgcg ctgacggtgc tcgtcctcgc cggccccggc gtgccgggcc
      601 acagcatcgc cgacggcaag atcgccgaga gccggaaccg cgcggacctc gcctactact
      661 tcggcatcca cctgttcccg ttctcgtggc accgcgacgg caagaagcac acgctgcccg
      721 gccgctactt cagcgccgac tacagcgact ggtacgaccg gatcagcgtg gtcgccggcg
      781 ccgacatcgc ccatccgacc gaggccggct acatcggcgg agcggtcgag gtctacggcg
      841 gggtctcgat caccgccggc tggcagccgc gcaagtacaa ccggctgcag tccggctacg
      901 cggtcggcga catgttcatg ggcgaccagg tcccgaccga cgcggtgtgg aagctcgacg
      961 gctgggccgt ggggatcagc atcgacgcgt cgctgctcaa gacgctgctc ggctacatcg
     1021 gcaggtagcg ggtcaccccg gtcaccccgg tcactccgct cgggccgcgg cctgcttgcg
     1081 ggcggcgcgc cgggcgcgca gcgcgagcat cggctgctcg accaggtagt acgacagcat
     1141 cgcggcgccg agcgcgaacg cgaggtggaa cgggaacacg ttgacgatcg agtcggcgtg
     1201 gcggttcacg aacggctgct gccacaggta gagcgagtag ctgagcgcgc cgatgaacac
     1261 cagcggccgc gactccagca cgcggccgac cgggagcgtc aggtgccgga cgcagcggtg
     1321 gatcgccatt gcgatcccga ggttgccgag cgtcacgccg aacgcgaggt tgaacccggc
     1381 gcggtggatg gacagcgcgt acacgcaggc cagcggcagg gtccagaacg cccatcccgg
     1441 cgcgtcgatc cacatcaggt aggtcggcga gctctccagc cactcgcggc cgagcgcgag
     1501 cacgcagccg gtggccagcg agtcgaacac gaacgggaac gcctgatcgg tgagcggacc
     1561 gatctcgggc cacagcttga acgcggtgac ccgcaggatc ggcgcagcga tgatcgcgcc
     1621 gatcgcgagc cacagcgcgc gctgcggccg cagcaccgac agcgcgaacg gccacaagag
     1681 gtagaactgc tcctcgacgg cgagcgacca caggtggccg agccaccagg tgcgctcggc
     1741 gtggaagttc atcgtgtagg tcgcggcgaa cgcgacgtcg tgggccggca gctcgatcca
     1801 gcccagcgcc gtgatggtca ggaggatcgc caggtagacg tagaacgccg ggaagatccg
     1861 gtagacccgg cggatgtaga agtcgcgcag cgagatccgc ccgcgcttcc ggtactcggc
     1921 gagcaggagc gtcgtgatca ggaaccccga gatcacgaag aacgtgcgga ccccgaggtc
     1981 gccgaacacc gagacctgga gccacgacgg cacgttgcgg gtgcccgccg agtgggcgca
     2041 caggacgatc aggatcgaga tcgcgcgcag gccatcgagc ccaggaatcc gaccacgcgt
     2101 gtcccgcaac ggacagccat tgtacgaggc cgcgcggccg cgctcacccg cgcgccagcg
     2161 ctcgacgggt gcgcaccagc gctcgacgcg caccagcgct caccagccga gccagccgct
     2221 ttgcagcgac agtgccagcg gctggccgat cgtctccatc cggccgcgcg ggccgagtcg
     2281 cgtgatccgc gcgcccgact gcggccggtg atcggtggcg gtatagccga tcgtggcgaa
     2341 gccctcgatg tcgacgctgt ggaaggactc catcgtgtcg cgcaggttgt cgccggtcac
     2401 cggcttgccc tggtcgagca gccgctcgac cgcgagcttc cacgtcgcga ccgcgacccg
     2461 gccgtagacg tactgcaccg tcttgtacgc gtcgggcgga tcgccgtcga tcgcgcggta
     2521 gcgggcgtgg tcggcgacca gccccgcgct gcccgccgcg gtgacatcgc cgaacagcgc
     2581 gaacggctgg acgacgtgga acccgacgca cgcgtcgccg caccggccgt acagcgactc
     2641 gtcgaccgac cagttgttgc cgatcacgtg gacgtcgatc ccgagctggg tcttgacctt
     2701 gtgcagcgcc ttgccgagcg acgccgcgtt ggggctggtg ttgccgaacc agacccagtc
     2761 ggcgatctcg tagtcggggt gggcggcctt gtagcgcagc tcgtcgtgga agaaccgcag
     2821 caccttgcgc tcgatcgccg gcgcgtcgtc ggcgagctcg acgaacaggt cgcggccgat
     2881 ccgggtgccg ccgagcgcct ggaggaacgt cttggcgccg tcgaccggat cggtgcagaa
     2941 cgcgctggtc gagcagtaga agaacgccat ccggcgcgcg ccctggcgcc acgcgaagct
     3001 cgccgcgacc cgcgccgcgg tggtgtagtc g
//
LOCUS       DASVOR010001157         4578 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362466,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001157 DASVOR010000000
VERSION     DASVOR010001157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4578
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362466"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VF516_22075"
     CDS             complement(<1..431)
                     /locus_tag="VF516_22075"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8110443.1"
                     /translation="MVTRDALRIEGHELTGVELAGYATGPELGTAPVVVVVGGITATP
                     FPFTGEDAWWPALAAEDLIDPVRHTVLAPCWPGNGSTWRGLSDDPLPPLSAAGLADLI
                     AAWLDGIGVTAPVWYVGASLGGMVGAALAVRHPERVARLIA"
     gene            complement(1013..2188)
                     /locus_tag="VF516_22080"
     CDS             complement(1013..2188)
                     /locus_tag="VF516_22080"
                     /inference="COORDINATES: protein motif:HMM:NF036995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HEX8110444.1"
                     /translation="MARKTTQATPEEPGYEAVLADVVRMVEAARRASARAVNAFITAT
                     YWTIGRRIVEQEQHGYARADYGAALVDRLAVDLTARFGRGFGRRNLFQMRALYLAYRE
                     IPQAVLAPPEGLPGAKKVQTPSALSTATTTVVGGTAAPTFPLPWSHYVRLLSVRSAEA
                     RRFYETEALRGGWTIRQLDRQIGTQFYERTALSRNKAAMLRRGEAQQPGDAVTPEEEI
                     KDPYILEFLALRDEYAETDLEAALIAQIETFLLELGGDFTFVGRQRRLRIGDTWYRID
                     LLFFHRRLRCLIIIDLKLDRFTHADAGQMHLYLNYAREHWMLEGENPPVGLVLCAEKD
                     SAVARYALEGLSNKVLAAEYRMTLPKERTLVAEIERTLQRFDARHRAPRRRVRPPRR"
     gene            complement(2515..>4578)
                     /gene="pstS"
                     /locus_tag="VF516_22085"
     CDS             complement(2515..>4578)
                     /gene="pstS"
                     /locus_tag="VF516_22085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX8110445.1"
                     /translation="MAIVVVLVFAMWWLGWWGGRAGGAGTAPPVAVSAEQALPVVAPP
                     AAPGSGEVHAARMKISNLTSVGILARTASVANAGRVTLNGSGSTFQLAYQEAAIEAYT
                     KTATVKINYGGGGSGKGRRDLADVVVDFAGADSPYKDADLAKNNGGDVLYFPILLGPI
                     TISYHLDGVAKLRFSPDTIAKIFQRDIKKWDDKAIASDNPGSKLPDADIVVVHRSDGS
                     ETTDNFTKYLVDAAGATWKLKSGSTVEWSADTQAGNGNPGVAQIVESTNGAIGYVDLS
                     DAKASGLAYAAIKNKAGKFVEPTSESASIAAEGIDIKDNLLFSALNAKGAAAYPITYQ
                     SWVIVYAKQADKDRGAALRGYLKFLISDGQKLLEDLDLAPLPKSLQDRALKQLDKIAA
                     SDAGQSGQQRAPAVVPAVVANTVGTQTPSRPHDDPEPVSIPVVTGTEPPRASPGRQGN
                     PAREDNVEALINQLTSSDNDKVRASAALRLAKLGDPRATAASGDQATPDEMRAAIEAL
                     SQASQDPSELVRSEAQKALRAILESRTGRKPSREVAIYVNVGPMRSNNEEARDNEGLK
                     SLMRSVATRTIEKLGLRTAWPGGPPTKDMLSADNTMGFYIDATLDALKVKKTDSSVIV
                     SCHIQLQLMSYPDKDAIGHLSGGASVQTSANDVALAEQDCVSVVIEDLVAKKVGPTIN
                     NKARR"
BASE COUNT          713 a         1563 c         1507 g          795 t
ORIGIN      
        1 atcgcgatca gccgggcgac gcgctcggga tggcgcaccg cgagcgcggc gccgaccatg
       61 ccgcccaggc tcgccccgac gtaccacacc ggcgcggtga cgccgatccc gtcgagccac
      121 gccgcgatca ggtcggcgag cccggcggcc gacagcggcg gcagcggatc gtcggacagc
      181 ccgcgccacg tcgagccgtt gcccggccag cacggggcga gcacggtgtg gcggaccgga
      241 tcgatcaggt cctcggcggc gagcgccggc caccacgcgt cctcgccggt gaacgggaac
      301 ggcgtggccg tgatcccgcc gaccaccacc acgacgggcg ccgtgcccag ctcgggcccg
      361 gtcgcgtagc cggcgagctc gaccccggtg agctcgtgac cctcgatcct cagggcatcg
      421 cgcgtgacca cgagccggcg cggctgcaga gcgtcggcgg aaacagacaa cggcgcggtc
      481 gcgggcacgc gggagcgaca ttacagcgcc gccgtcgatc cgcaagcccc aaacgccccg
      541 cgcgcgatgc gtggtcacgg ctgcgatgcc ggtgcgcgac gcacgccgtc gcccagtcac
      601 cgcggcacgc gcggtctgct agcctcggcg ggccgtggac gactcgctcc ggcaccggac
      661 gcagacggcc cgtgccgaga tccgctccgg cgcgttgcgc ggcaaccgac ccgctgccgc
      721 cgatcgggcc gctgttcgtc ggcagcgagg aacggcacgt gcgatcgaac gacgacctcg
      781 cgagccaaga tcggaggctc cccaggtcgc cggtgccctc gaactcgcgc gggcccgcac
      841 ctgtcgccac cgcgtcgcgc gagtgcgatg ccgcccgaca ggtcgcgtcg cgaaattggc
      901 aaccatgtca acttgccgac gaagcgctcg tgatcttcgt agggttgcca catggtccgc
      961 ggcgcgaggc gtgatccacg tagagccacg tggcgatctg ggcgacctgt cgtcagcgcc
     1021 gcggcggtcg cacgcgccgt cgcggcgcgc ggtggcgtgc gtcgaaccgc tggagcgtcc
     1081 gctcgatctc tgcgacgagc gtgcgctcct tggggagcgt cattcgatac tcagccgcca
     1141 gcaccttgtt cgaaaggccc tcgagagcat agcgcgcgac ggcgctgtct ttttccgcgc
     1201 acagaacgag ccccaccggc ggattctcgc cctcgagcat ccagtgctcc cgcgcgtagt
     1261 tcaggtagag gtgcatctga ccggcgtccg catgggtgaa ccgatccaac ttcaagtcga
     1321 tgatgatgag acaccgcaga cgacgatgga agaacagcag atcgatccga taccaggtgt
     1381 cgccgatacg aaggcgacgc tgccggccga cgaaggtgaa gtcgccgcca agctccagca
     1441 agaaggtctc gatctgggcg atcagcgccg cctcgaggtc ggtctcggcg tactcgtccc
     1501 ggagcgccaa gaactcgagg atgtacgggt ccttgatctc ctcctcggga gtcaccgcat
     1561 cgccgggctg ctgcgcctcg ccacgccgca acatcgcagc cttgttccgg gacagcgccg
     1621 tacgctcgta gaactgggtg ccgatctggc gatcgagttg ccggatggtc cagccgcccc
     1681 ggagcgcttc ggtctcgtag aaccgccgtg cttcggcgct gcggacgctc agcagacgca
     1741 cgtagtgcga ccagggcagc gggaaggtgg gcgctgcggt ccccccgacg acggtggtcg
     1801 tcgccgtgga aagtgcagac ggcgtctgca ctttcttggc cccggggagt ccctcgggtg
     1861 gagcaagcac ggcctgcggg atctcgcggt aggcgaggta cagggcccgc atctgaaaca
     1921 ggttccggcg cccgaacccc cgaccgaacc gcgcggtgag gtcgaccgca aggcggtcca
     1981 ccagcgccgc accgtagtcg gcgcgcgcgt acccgtgctg ttcctgctcg acgatccggc
     2041 gaccaatggt ccagtacgtt gcggtgatga acgcattgac ggcccgcgcc gatgcgcgcc
     2101 gtgctgcctc gaccatccgc acgacgtccg cgagcacggc ttcatagccg ggctcttcgg
     2161 gcgtcgcctg ggtcgtcttc cgcgccacgg tctcaacact gccccgagga tcgctccgac
     2221 gacaagtcgg cgtgcaggat tcctgtcgcc gcgtggtgat cccggccttg acgtcatgcc
     2281 gacgatgcgg gcctcgatgc gagcgaagca acacgacggc gcgcacgatc tggatgcgcg
     2341 gatcgatgcg aacgtccgca ggcgtgtcgg ggcaacggcc gccacggcgg cgagaggtcg
     2401 gccgcgcgcg tctgcagcgg agatcgcgcc atcggcctcg ttcacacgct cgaccaggag
     2461 ccaggtccgg attcgggaga cgatcgttgc gtcgcggcga cagcgtatca caccctatct
     2521 tcgcgcctta ttattgatcg ttggaccgac cttcttcgcc accaagtcct cgatcacgac
     2581 ggacacgcag tcctgttctg ctagcgcgac gtcgttcgcg ctggtctgta cgctagcccc
     2641 gccactcagg tgtccaatgg cgtccttgtc tggatagctc atcagctgca actggatatg
     2701 gcaggacacg attacgctcg agtcagtctt tttcaccttg agtgcatcca acgtggcatc
     2761 gatataaaag cccatcgtgt tgtcggcgct gagcatgtcc ttggtgggcg gaccacccgg
     2821 ccacgcggtc ctcaaaccga gcttttctat ggttctggtg gcgacgctcc tcatcaggga
     2881 cttgagcccc tcgttgtctc ttgcctcctc gttattcgag cgcatcgggc cgacatttac
     2941 gtagatcgcg acctcgcgcg agggtttccg gcctgtacgg ctctccaaga tcgcccggag
     3001 cgccttctgg gcctcgctcc tcacgagctc gctgggatct tggctcgctt gactcagcgc
     3061 ctcgatcgct gctcgcatct cgtcgggagt ggcttggtcg ccagaggcgg ccgtcgcacg
     3121 cgggtcgccg agcttggcga ggcgaagcgc tgctgacgcc cgcaccttgt cgttgtcact
     3181 gctcgtgagc tggttgatca gggcctcaac gttatcttcg cgggcggggt tcccctgtcg
     3241 tcccggggag gcgcgtggag gttcggttcc cgtcacgaca gggatggaaa ccggctcagg
     3301 atcgtcgtgc ggccgcgatg gcgtctgagt tcctaccgta ttggccacga ccgctgggac
     3361 gacggctggc gcacgttgct gaccgctctg acctgcgtcg gaggctgcga tcttgtcgag
     3421 ctgcttcagt gcccgatcct gcaagctctt gggcagcggc gccagatcga gatcctcgag
     3481 gagcttctgt ccgtcactga tgaggaactt gagataccct ctgagcgcgg cccccctgtc
     3541 cttgtcggcc tgcttggcat agacgatcac ccagctctgg tacgtgatcg ggtaggcggc
     3601 ggcgcccttc gcgttcagtg cgctgaacaa gaggttgtcc ttgatgtcga tgccttcggc
     3661 cgcgatcgac gcagactccg aagtaggctc gacgaacttg ccggccttgt tcttgatggc
     3721 cgcgtacgcc agtccggacg ccttggcgtc ggacaggtcg acgtagccaa tcgcaccgtt
     3781 ggtcgactcg acgatctgcg cgacgccggg gttgccgttg ccggcctggg tgtcagccga
     3841 ccactcgacc gtcgagccgc tcttcagctt ccacgtggca ccggcagcgt cgacgagata
     3901 cttcgtgaag ttgtcggttg tttccgagcc gtccgagcga tggacgacga cgatgtcggc
     3961 gtcgggcaac ttactccctg gattgtcgga cgcaattgcc ttgtcgtccc acttcttgat
     4021 gtcgcgctgg aagatcttgg caatcgtgtc cggggaaaag cgaagtttgg cgacgccatc
     4081 gagatggtag ctgatcgtga tcgggccgag caggatcggg aagtacagga catccccgcc
     4141 attgttcttg gcgaggtcgg cgtccttgta cggcgagtcc gcaccggcga agtcgaccac
     4201 cacgtcggcg agatcccggc ggccctttcc gctgccgccg ccgccgtagt tgatcttgac
     4261 ggtcgcggtc ttggtgtagg cctcgatcgc ggcctcttga taggccaact ggaacgtcga
     4321 gccgctaccg ttcaatgtca cgcggcccgc gttcgcgacg ctcgccgtcc tggcgaggat
     4381 gccgacgctg gtcaagttgg agatcttcat tcgggccgcg tgcacctctc ccgaccccgg
     4441 cgctgccggt ggcgcgacca ccggcagcgc ttgctcggcc gacaccgcca ccggcggcgc
     4501 cgtcccggca ccgcccgcgc gaccacccca ccagccgagc caccacattg caaacaccag
     4561 caccaccacg atcgccat
//
LOCUS       DASVOR010001158         1863 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_128386,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001158 DASVOR010000000
VERSION     DASVOR010001158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1863
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128386"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..833)
                     /locus_tag="VF516_22090"
     CDS             complement(<1..833)
                     /locus_tag="VF516_22090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110446.1"
                     /translation="MTSAPAIPPRLRRSRHISWFAHMGAVYLFHDLYGYLMQMSPDIA
                     GMIEAFSDGVDTAETIEYFQGKFDGAEPRQLVDVLAAHAVLVDPGEDEVEAMWAFVPI
                     KARWNVWQRRGDRLTLWTAWGDRPVQQLFLDADETRIWDALDGQKRLIELRHHHDNAR
                     LLGLVRRLVHHDVQALKLSMMPWSVYAKRPAMAPAYLASTMPYPSWQPGTPVPPEPAL
                     DAYHQTGIADADAQFDHQETTLSHLLRVPHPVLAAPVGGDGGGRAPRTYGQALIDSLL
                     AR"
     gene            872..>1863
                     /locus_tag="VF516_22095"
     CDS             872..>1863
                     /locus_tag="VF516_22095"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8110447.1"
                     /translation="MPYLFETEDHAAIRASARRFAQAHIAPHGAAWEEAEEFPVELYR
                     TAAAAGVIGVGYPEAIGGQGGDLGHVLVASDELVLAGRSVGTIVGLGSHGIALPPIVR
                     FGTPDQIRRFVTPCLTEGKISALAVTEPGGGSDVAALATRAERDGDHYVVTGAKTFIT
                     SGTRADFVTCAVRTGGPGHGGISLVVIERGTPGFVVAGKLRKTGWWASDTAELRFDGC
                     RVPVAHRIGPEHGAFPMIMMNFAAERLMLAGQCVAIAELAYRESIAYAQGRSAFGKPL
                     TGFQVTRHKLADMASRIAAARALTGEAVTRVLRGEQAAGLAAMAKNVATDMCSFV"
BASE COUNT          250 a          680 c          672 g          261 t
ORIGIN      
        1 cgggcgagca ggctgtcgat cagcgcctgg ccgtaggtcc gcggggcgcg gccgccgccg
       61 tcgccgccga ccggcgccgc cagcaccgga tgcggaacgc gcagcaggtg ggacagcgtg
      121 gtctcctggt gatcgaactg cgcgtcggcg tcggcgatgc cggtctggtg gtacgcgtcg
      181 agcgcgggct cgggcggcac cggcgtgccg ggctgccagc tcgggtacgg catcgtcgag
      241 gccaggtagg ccggcgccat cgccggccgc ttggcgtaca ccgaccacgg catcatcgac
      301 agcttcagcg cctggacgtc gtggtggacc agccgccgga ccaggccgag cagcctggcg
      361 ttgtcgtggt ggtggcgcag ctcgatcagc cgcttctggc catcgagcgc gtcccagatc
      421 cgggtctcgt cggcgtcgag gaacagctgc tggaccggcc ggtcgcccca cgcggtccac
      481 agcgtcagcc ggtcgccgcg ccgctgccac acgttccacc gcgccttgat cggcacgaac
      541 gcccacatcg cctcgacctc gtcctcgccg ggatcgacca gcaccgcgtg ggcggccagc
      601 acgtcgacca gctggcgcgg ctcggcgccg tcgaacttgc cctggaagta ctcgatggtc
      661 tcggcggtgt cgaccccgtc ggagaacgcc tcgatcatgc cggcgatgtc cgggctcatc
      721 tgcatcaggt agccgtacag atcgtggaac aggtacaccg cgcccatgtg cgcgaaccac
      781 gagatgtggc gcgagcgccg gagccggggc gggatggccg gagcggaggt catactcgtt
      841 cgtagcactt gtcggtatgc tgcgtggcca tatgccttac ctgttcgaga ccgaagacca
      901 cgccgcgatc cgggcgagcg cgcggcggtt cgcgcaggct cacatcgcgc cgcatggcgc
      961 ggcgtgggag gaggccgagg agttccccgt agagctctac cgcacggcgg cggcggccgg
     1021 ggtcatcggc gtcggctacc ccgaggcgat cggcgggcag ggcggcgatc tcggtcacgt
     1081 gctggtcgcc tccgacgagc tggtgctcgc cggccgctcg gtcggcacca tcgtcggcct
     1141 cggcagccac ggcatcgcgc tgccgccgat cgtccggttc ggcacgcccg accagatccg
     1201 ccggttcgtc accccgtgcc tcaccgaggg caagatctcc gcgctcgcgg tcaccgagcc
     1261 gggcggcggc agcgacgtcg cggcgctcgc gacccgcgcc gagcgcgacg gcgaccacta
     1321 cgtcgtcacc ggggccaaga ccttcatcac ctccggcacc cgcgccgact tcgtgacctg
     1381 tgcggtgcgc accggcggcc cgggccacgg cgggatctcg ctcgtggtga tcgagcgcgg
     1441 cacccccggc ttcgtggtcg cgggcaagct gcgcaagacc ggctggtggg cgagcgacac
     1501 cgccgagcta cggttcgacg gctgccgcgt gcccgtcgcc caccggatcg gccccgagca
     1561 cggcgcgttc ccgatgatca tgatgaactt cgccgccgag cgcctcatgc tcgccggcca
     1621 gtgcgtcgcg atcgccgagc tcgcctatcg cgagtcgatc gcctacgcgc agggccgctc
     1681 cgcgttcggc aagccgctga ccgggttcca ggtcacccgc cacaagctcg ccgacatggc
     1741 gagccggatc gccgcggcgc gcgcgctgac cggcgaggcg gtgacccgcg tgctgcgcgg
     1801 cgagcaggcc gccggcctgg ccgcgatggc caagaacgtc gcgaccgaca tgtgcagctt
     1861 cgt
//
LOCUS       DASVOR010001159         1550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_567319,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001159 DASVOR010000000
VERSION     DASVOR010001159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567319"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1111
                     /locus_tag="VF516_22100"
     CDS             146..1111
                     /locus_tag="VF516_22100"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX8110448.1"
                     /translation="MTELDMALVTDARRALDGRIRRTPIEASPALSDAAGAPVFLKLE
                     ALQLTGSFKIRGAWFVLSRLSADERQRGIVTCSAGNHGKATARVAHELGIPAEIHVPR
                     DVDPAKYQAIVALGARVIRSDFEGYDDTERLARAAAAQSGRPFLSAFDDPCILAANGG
                     TIAAEVVEDCPEARSFLLPVGGGGLAGGFAYYAKAVLGHCRVIGCQHEGSPALALSLA
                     RGEAVTTLPAYPTTAGGLEGGIGRIGFEALRSRVDRVALLGEAEIFDAIRFVLEHHGY
                     LIEPSAAVVVAAILTGKAGRLESPTALVLSGRNVALSTLRRILAA"
     gene            1108..>1550
                     /locus_tag="VF516_22105"
     CDS             1108..>1550
                     /locus_tag="VF516_22105"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110449.1"
                     /translation="MKVIGPDEIRRRIGAAQAIAAMRDAVLAQARGECDTPMPMHLDL
                     SAAGGGEVHIKSSYRRGGRHVALKVAGTYAERPYGSIALISAATGETVAYLDDGGYLT
                     DLRTAAVAALVARELGRADRALGILGTGVQARLLAELHAEVLPLE"
BASE COUNT          190 a          600 c          558 g          202 t
ORIGIN      
        1 gtcgagcgct gcgcgatgcc ggtgacaacg acgatcagaa ctcccacccg gaagcgtccc
       61 cacatggcgg cgattctagc cgatcgcaag gcccccggcg atcccgcgct ttgcgccggg
      121 cgcgggtgcg gctagcatcg ccgccgtgac cgagctcgac atggccctcg tgaccgacgc
      181 gcgccgggcg ctcgacgggc ggatccggcg caccccgatc gaggcgtcgc ccgcgctgtc
      241 ggacgccgcc ggcgcaccgg tgttcctcaa gctcgaggcg ctccagctca ccggctcgtt
      301 caagatccgg ggcgcctggt tcgtgctgtc gcggctgtcg gccgacgagc gccagcgcgg
      361 gatcgtgacc tgctcggcgg gcaaccacgg caaggcgacc gcccgcgtgg cgcacgagct
      421 cggcatcccg gccgagatcc acgtcccgcg cgacgtcgac ccggcgaagt accaggccat
      481 cgttgccctc ggcgcccggg tgatccggtc ggacttcgag ggctacgacg acaccgagcg
      541 gctcgcgcgc gccgccgccg cgcaatcggg ccggccgttc ctgagcgcgt tcgacgaccc
      601 ctgcatcctc gccgccaacg gcgggacgat cgccgccgag gtcgtcgagg actgccccga
      661 ggcgcggagc ttcctcttgc cggtcggcgg cggcgggctg gcgggcgggt tcgcgtacta
      721 cgccaaggcc gtgctcggcc actgccgcgt gatcggctgc cagcacgagg gctccccggc
      781 gctcgcgctg tcgctggcgc gcggcgaggc ggtcaccacc ctgcccgcgt atccgaccac
      841 cgccggcggc ctcgagggcg gcatcggccg gatcggcttc gaggccctgc gatcgcgggt
      901 cgaccgcgtc gcgctgctcg gcgaggccga gatcttcgac gcgattcgct tcgtgctgga
      961 gcaccacggc tacctgatcg agccgtcggc ggcggtcgtc gtcgccgcga tcctgaccgg
     1021 caaggccggc cgcctggagt cgcccaccgc cctggtgctg tcgggccgca acgtcgcgct
     1081 gtccaccctt cgccggatcc tcgccgcgtg aaggtcatcg gccccgacga gatccgccgc
     1141 cggatcggcg cggcgcaggc gatcgcggcg atgcgcgacg cggtgctcgc ccaggcccgc
     1201 ggcgagtgcg acacgccgat gccgatgcac ctcgacctgt cggcggccgg cggcggcgag
     1261 gtccacatca agtcgagcta ccgccggggc ggccggcacg tcgcgctcaa ggtcgccggg
     1321 acctacgccg agcggccgta cggatcgatc gcgctgatct cggcggcgac cggcgagacc
     1381 gtcgcctacc tcgacgacgg cggctacctg accgacctgc gcaccgcggc ggtcgcggcg
     1441 ctggtcgcac gcgagctcgg ccgcgccgat cgcgcgctcg gcatcctcgg gaccggcgtc
     1501 caggcccgcc tcctggccga gctccacgcc gaggtgctgc cgctcgagca
//
LOCUS       DASVOR010001160         3913 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_304037,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001160 DASVOR010000000
VERSION     DASVOR010001160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304037"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            194..2275
                     /locus_tag="VF516_22110"
     CDS             194..2275
                     /locus_tag="VF516_22110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8110450.1"
                     /translation="MPAINAVVHLAADGDIAILSVNYPPVNALSQQVRAGLLDGVRQA
                     TADAAVRAIVLICDGKTFTAGADITEFGKPPQPPPLVEVQDAIEGSPKPVVAAIHGTA
                     LGGGLELALCCHYRVAVPSAKCGLPEVNLGLLPGAGGTQRLPRIVGVEQALDMVTSGK
                     HIDARTAHAAGLIDELVPEDALRASAIAFARRLVAEQRPLKRVRDLDDKLAAARGKPE
                     IFDGFRKANAKKFRGFLAPEYNIRSVEAAVDKPFAEGLAIERKLFTELMASTQSAAQR
                     YVFFAERQVWKIPDVPDDTPTIPVKKVGIIGAGTMGGGIAMNFVNLGMPVTIVETKQD
                     ALDRGLGVVRKNYERSTKSGKLTMADVDKRMQLFTGTLDLGQLADCDLVIEAVFENMD
                     IKKEVFGKLDRIVKPGAILATNTSALDINEIATAVQRPEAVIGLHFFSPANVMRLLEV
                     VRARATAKPVIATSMQLARKLGKIAALVGVCPGFVGNRILYARSQQANAMLMEGAMPW
                     DIDKAIYDFGLPMGPFAMSDLAGLDIGWNPEKTRSSTIREVLCEQNRRGQKTGAGFYD
                     YDEQRNAKPSPVTEKIIREFAAKSGNPQRTITEQEILERCLYAQINEGAKILEEKIAI
                     RPSDIDIVWINGYGWPVYRGGPMFYADTVGLPQVVARLEEYGPRLGKDFAISPLLRRL
                     AAEGKRFQDMK"
     gene            2458..3462
                     /locus_tag="VF516_22115"
     CDS             2458..3462
                     /locus_tag="VF516_22115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110451.1"
                     /translation="MVSRVPLLRQCQPGDLIDGRYRIAQKLAEGGMGVVFLAEHVLIR
                     RRLAIKLLHAELAPDRWMVQRFLHEAAASGSLVHPHIVESTDMGFTREGIPYIVFEYL
                     EGCLLTEEIRRLGRLSLGRTLAIARQIASALHTVHEAQLAHLDVKGDNVLLTRRSGSA
                     DHAKLIDFGISRFTSSSGQKAPPGILMGTPEYMAPEQVIRPESADGRADIYALGVLIY
                     EMLCGRCPFEGDDSRRVLARVLHDEPPMLDRPVPPLLEQLVFDGLLVKSPERRLQTIA
                     EILPVLDALLAASRSAPEADGFDAPAPARSGPLITPTWALEPVIEDVISLGGDLDAPA
                     "
     gene            3529..>3913
                     /locus_tag="VF516_22120"
     CDS             3529..>3913
                     /locus_tag="VF516_22120"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX8110452.1"
                     /translation="MLRGTLRWLAALSLALNAVLVGLWAVRHLLPQPVPWLRERARGD
                     LFHALATGTVTRRDVVVVGDSLTECGEWWELLDRPVANRGVAGDTVAAVRARLDDIVA
                     MDPRVVVVGIGVNDLLAGASPEALAA"
BASE COUNT          591 a         1390 c         1381 g          551 t
ORIGIN      
        1 ggcgcggccg ggttcgcgcg atcgcgcggc cgatcgcggt ccgggaccag gcgccacgcg
       61 gcgaggccca gcagcaccag cacgaccagc ccgagcaacg tcttcgtctt cacgaccagc
      121 tcccgggccg aatatgccac tgcgcacccg tcgtgcccag tacgccccgc ggtttggccg
      181 tatagtccgc gccatgcccg ccatcaatgc cgtggtccac ctcgccgccg acggtgacat
      241 cgcgatcctg tcggtcaact atcccccggt gaacgcgctg tcccagcagg tgcgcgccgg
      301 actgctcgac ggcgtccgcc aggcgaccgc cgatgccgcg gtccgggcga tcgtgctgat
      361 ctgcgacggc aagacgttca ccgccggcgc cgacatcacc gagttcggca agccgccgca
      421 gccgccgccg ctggtcgagg tccaggacgc gatcgagggc tcgcccaagc cggtggtcgc
      481 ggcgatccac ggcacggcgc tcggcggcgg cctcgagctc gcgctgtgct gccactaccg
      541 ggtcgcggtg ccgtccgcga agtgcgggct gcccgaggtc aacctgggcc ttctgcccgg
      601 cgccggcggc acccagcgcc tgccccggat cgtcggcgtc gagcaggcgc tcgacatggt
      661 gaccagcggc aagcacatcg acgccaggac cgcacacgcg gccgggctga tcgacgagct
      721 ggtccccgag gatgcgctgc gcgcgagcgc gatcgcgttc gccaggaggc tggtcgccga
      781 gcagcggccg ctcaagcggg tgcgcgatct cgacgacaag ctcgccgccg cgcgcggcaa
      841 gccggagatc ttcgacgggt ttcgcaaggc caacgccaag aagttccgcg gcttcctcgc
      901 gcccgagtac aacatccgct cggtcgaggc cgcggtcgac aagccgttcg ccgagggcct
      961 ggcgatcgag cgcaagctgt tcaccgagct gatggccagc acccagtcgg cggcgcagcg
     1021 ctacgtgttc ttcgccgagc gccaggtctg gaagatcccc gacgtgccgg acgacacgcc
     1081 gaccatcccg gtcaaaaagg tcggcatcat cggcgccggc acgatgggcg gcggcatcgc
     1141 gatgaacttc gtcaacctcg gcatgccggt cacgatcgtc gagaccaagc aggacgcgct
     1201 cgaccgcggg ctcggcgtgg tgcgcaagaa ctacgagcgc agcaccaaga gcggcaagct
     1261 gacgatggcc gacgtcgaca agcgaatgca gctgttcacc gggacgctcg acctcggcca
     1321 gctcgccgac tgcgatctgg tgatcgaggc ggtgttcgag aacatggaca tcaagaagga
     1381 ggtgttcggc aagctcgacc ggatcgtcaa gccgggcgcg atcctggcga ccaacacgtc
     1441 ggcgctcgac atcaacgaga tcgcgaccgc ggtccagcgg cccgaggcgg tgatcggcct
     1501 gcacttcttc tcgccggcca acgtgatgcg cctgctcgag gtcgtgcgcg cccgcgcgac
     1561 cgccaagccg gtgatcgcga cgtcgatgca gctcgccagg aagctgggca agatcgccgc
     1621 gctggtcggc gtgtgcccgg gcttcgtcgg caaccgcatc ctgtacgcgc gctcgcagca
     1681 ggccaacgcg atgctgatgg agggcgcgat gccgtgggac atcgacaagg cgatctacga
     1741 cttcggcctg ccgatgggcc cgttcgcgat gtcggacctc gccgggctcg acatcggctg
     1801 gaaccccgag aagacccgca gctcgacgat ccgcgaggtg ctgtgcgagc agaaccgccg
     1861 cggccagaag accggcgcgg ggttctacga ctacgacgag cagcgcaacg cgaagccgtc
     1921 gccggtcacc gagaagatca tccgcgagtt cgccgcgaag tcgggcaacc cgcagcgcac
     1981 gatcaccgag caggagatcc tggagcgctg cctgtacgcc cagatcaacg agggcgccaa
     2041 gatcctcgag gagaagatcg cgatccggcc gtcggacatc gacatcgtgt ggatcaacgg
     2101 ctacggctgg ccggtgtacc gcggcgggcc gatgttctac gccgacaccg tcggcctgcc
     2161 ccaggtggtc gccaggctcg aggagtacgg gccgcggctc ggcaaggact tcgcgatctc
     2221 gccgctgctc cgccggctcg cggccgaggg taagcggttc caggacatga agtaatcagg
     2281 cgacgcgatg cgtcgccccg ggcgacctcg cgcgagtgcg ccgctgaatc gcgcgccctc
     2341 gcccggggac gatgtaggcc ggcttgcgcg gcgggtcggg cgactcgtcg atgcgccggc
     2401 ttgcacgacg ccggcgcgcc gccgcggctg gtcggcttcc tgcagatggc ctaaaagatg
     2461 gtctcgcggg tccctctcct tagacagtgt cagcccggcg atctgatcga cggacgatac
     2521 cggatcgcgc agaagctcgc ggagggtggg atgggggtgg tgttcctcgc cgaacatgtg
     2581 ctgatccggc ggcggctcgc gatcaagctg ctgcacgccg agctcgcccc ggaccgctgg
     2641 atggtgcagc gcttcctgca cgaggccgcg gcctccggca gcctggtcca cccgcacatc
     2701 gtcgaatcca ccgacatggg attcacccgc gagggcatcc cctacatcgt gttcgagtac
     2761 ctggagggct gcctgctcac cgaggagatc cgccggctcg gccggctgtc gctcgggcgc
     2821 acgctggcca tcgcgcgaca gatcgcctcg gcgctgcaca ccgtgcacga ggcccagctc
     2881 gcccacctcg acgtcaaggg cgacaacgtg ctcttgaccc ggcgcagcgg atcggcggat
     2941 cacgccaagc tgatcgactt cgggatctcg cggttcacct cgagctcggg ccagaaggcg
     3001 ccgcccggca tcctgatggg gaccccggag tacatggcgc cggagcaggt gatccggccc
     3061 gagtcggcgg acgggcgcgc cgatatctac gcgctcggtg tgctgatcta cgagatgctg
     3121 tgcgggcgct gtccgttcga gggcgacgat tcgcgccgcg tgctcgcccg ggtcctccac
     3181 gacgagccgc cgatgctcga tcgcccggtg ccgccgctgc tcgagcagct ggtgttcgac
     3241 ggcctgctcg tcaagtcgcc cgagcggcgg ctgcagacca tcgcggagat cctccccgtg
     3301 ctcgacgcgc tgctcgcggc gtcgcggtcc gcgccggagg cggacggctt cgacgcgccc
     3361 gcgccggcgc ggtcgggccc gctcatcacg ccgacctggg ccctggagcc ggtgatcgag
     3421 gacgtgatct cgctcggcgg cgacctcgac gcccccgcgt agcgcgcgac ccgcgtacca
     3481 tcgccggccg atgccgggac ccgaacggtg cgcggaatga tgcgcgggat gctgcgcggg
     3541 acgctgcgat ggctcgccgc gctgtcgctc gcgctcaacg cggtgctcgt ggggctgtgg
     3601 gccgtgcgcc acctcctccc gcagccggtc ccatggctgc gcgagcgggc gcgcggcgat
     3661 ctgttccacg cgctcgccac cggcacggtg acgcgccgcg acgtcgtggt cgtgggcgac
     3721 agcctgaccg agtgcggcga gtggtgggag ctcctcgatc gcccggtcgc caaccgcggc
     3781 gtcgccggcg acaccgtcgc ggccgtgcgc gcgcggctcg acgacatcgt ggccatggac
     3841 ccgcgcgtcg tggtcgtggg gatcggggtc aacgacctgc tggccggggc ctcgcccgag
     3901 gcgctggcgg cgc
//
LOCUS       DASVOR010001161        12573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_69980,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001161 DASVOR010000000
VERSION     DASVOR010001161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12573
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69980"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VF516_22125"
     CDS             <1..888
                     /locus_tag="VF516_22125"
                     /inference="COORDINATES: protein motif:HMM:NF020920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110453.1"
                     /translation="SLNYLLSSHVWRQDHNGFSHQDPGFIDHVVNKKAEVIRVYLPPD
                     GNTLLVVTDHCLRSRDCVNVIVAGKQPAPQWLTMDEAIKHCQSGIGIWSWASNDESDE
                     PDVVMACCGDVPTLETLATVSMIRLHAPQLKVRVINVVDLMRLQSSDEHPHGLSDHEF
                     DALFTKDKPIIFAFHGYPSLIHQLTYRRSNHRNFHVHGYREEGTTTTPFDMVVLNRLD
                     RFHLALDVIDRLPQLGSRAAYFRQAIRDALIDHKQYIQEHGEDMPEITGWRWERGATP
                     ESPRGVSRGAATSTEGDNV"
     gene            885..3062
                     /locus_tag="VF516_22130"
     CDS             885..3062
                     /locus_tag="VF516_22130"
                     /inference="COORDINATES: protein motif:HMM:NF015588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110454.1"
                     /translation="MTVAPDRREAPFRAAQGSIAWQVEAEGFDPAREHAIESMFAVGN
                     GYLGVRHVLPLPGAWGDLFVAGVYDRKDEGLAYSELEFLAPERGDSVHVQLEPLPYPL
                     HASVRVEGELLAIPGANARAMRRRLDLRCAVVEVEGVHETEGGRRTRLSSLHCASLAD
                     PHVLLQQVTAAAENHWGTIEITSSLFDPVEAHRDPPVVVLEHIRSGDLELIRYATHAS
                     GYEVCIAARAFTREDGRDAVVYRRVLAVFTNRDVQDPRAAALARIEQLAADAFDALLG
                     DHERAWREFWEDADIRVDGSPATEQALRFTMYHLRIAVDRDPGASIPGRAFTGRAYEG
                     HVFWDTEIFMLPFYLYTAPPLARNLLRYRRHTLDGARARARSLGYAGACFAWESTVTG
                     EDVTPTRIILKTTRQEIPIFTGTQQVHVTADIAYAVWRYWEATHDEAFLAGEGADLLF
                     ETARFWASRVTRDERGYHIRQVVGPDEYHHDVDDNAYTNWMARFNLERAAWLAGSLGS
                     AARPRLGADEPSRWQEVAAGLCCAGPDDRGVIEQFAGFHALEDYRLSPQERFKAPVSR
                     LFHWDKVNRLKLIKQPDVLMLLMLFPEAFPRDVVAANYRYYEPLTDHGSSLSAPVHAA
                     TAVRLGLYEDAERYWKQSLWFDLSNRMQNSDLGIHPAAMGGVWQALVFGWLGVRFTSD
                     GPQVDAAPAVRLPAQWRAVALKLAWRGRTYPVARSAEMPGGPP"
     gene            3059..3958
                     /locus_tag="VF516_22135"
     CDS             3059..3958
                     /locus_tag="VF516_22135"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8110455.1"
                     /translation="MSSFDSILLPLDGSLQAAKAVPSALWLAERLHATLHVLHAAPHP
                     LAPANALERLSALAAQRSRTVLHQTKVDPVQAVLEATKQHHVKLVVITARGESASRDV
                     ERGRTLGRIARALVVESSVPVLLVPLHDRSVLPWRSMLVAASGDPESDGALAAAARLA
                     SALDLRVTVVHVIAEPGSGGLGGYADAAYHELPWRLEEMVKRALLSSSPEDVGRIEGL
                     AVRRGDPITEVIAEVEQRRSSVVAVGWHGDLSPEHANVLLGLLERAAFPLLVVRKPPP
                     PEFRLKVGDEIGPRPDSTEGDHV"
     gene            3993..5192
                     /locus_tag="VF516_22140"
     CDS             3993..5192
                     /locus_tag="VF516_22140"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX8110456.1"
                     /translation="MRVVLVNAGSSSLKCSLMESADRTVLAHVQADWAGPVTRYERSG
                     PGGARIREQAGFREHGEAVRHALDDLRAASAPTAERPAAVGHRIVHGGEFSAPVRITP
                     AVRSRISALAELAPLHNPPGLNALAAAEALLPDIPHIAVFDTAFHTTLPPAARTYPLP
                     HAWTESWGIRRYGFHGLNASYCTTRAAELLGRPASELRLVICHLGHGCSATAVLHGHS
                     VDTTMGFTPLDGLMMATRSGSVDPGISAHVQRCHGLGAEEIDEALNHTSGLFGVSGLS
                     GDMREVLAAGRAGHERARLAIAIYVHRVRQAIGALAATLGGVDVLVFTAGVGENSAEV
                     RMASCQGLEHLGIELDADANARCHPDADVAGWTAHARVLVITAREDLAMLSDVVRVIA
                     TAKELNG"
     gene            5189..6250
                     /locus_tag="VF516_22145"
     CDS             5189..6250
                     /locus_tag="VF516_22145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF012496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX8110457.1"
                     /translation="MMDLTQTTADLTQPGKGILAADETVSTLTKRFDALDIASTSSSR
                     RDYREMLLTTSGVREFISGAILQDETIHQHSSTGAPIAALCSQLGMLPGIKVDAGTRP
                     LAAHPGEQVTEGLDGLRDRLVEYRGLGARFAKWRAVFAVTDTLPSSACTSANAGALAR
                     YAALCQEQELVPIVEPEVLMDGAHTIERCEEVTGCVLHAVFEALVEQGVRLEEMLLKP
                     NMVVPGDDSPQQADAGTVAAATLRCLRRHVPAAVPGVVFLSGGQRDVDATRHMDAINR
                     AGGPKPWALSFSYGRALQDAALAAWRGRPENRVPAQTKFYNRARCNGLAALGRYDERE
                     DSQQARGPEPRASAGWQDD"
     gene            6262..7104
                     /locus_tag="VF516_22150"
     CDS             6262..7104
                     /locus_tag="VF516_22150"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX8110458.1"
                     /translation="MDKDRPISAVLTDVDGTLVTKDKVLTERALGAVKQLRDRGIVFT
                     ITSGRPPFGMRMLVEPLGLTTPMAAFNGGAIVSPDLSVLDERPLPDALLPALVDMIQA
                     HGLDVFLFRTDDWFVRSLDAPRVHREASTIQRLPTVVPSFDSVLAGVVKVVGVSDDHP
                     RVAACEAAVQQAYGAQVSAARSQPYYLDVTNPTANKGVVIERLSHYIAIPLERIAAIG
                     DQPNDVLMFERAGLSIAMGNASDEVKRRATFVTTSFADEGFANAIEQIVLPRAEPAGA
                     RDQW"
     gene            complement(7095..7793)
                     /locus_tag="VF516_22155"
     CDS             complement(7095..7793)
                     /locus_tag="VF516_22155"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8110459.1"
                     /translation="MNILAIDIGGTNAKMLVSGKLEPRKFPSGPKLTPDKMVAGVLEL
                     VNDWEYDVVSIGYPGQIRGGRIVAEPYNIGKGWVGYDFQRAFGRPVKLINDGAMQALG
                     CYEGGALLFIGLGTGVGACLIVDGTSVPMEIGHLPYRKKTIEHYLGSAGLKRIGRKKW
                     QKTLEIVVEQLMAAFHPDRVVIGGGNAMKLKRLPPGCEAGDNAFALEGGFRLWQGEAR
                     AVAGSTEHREAGYH"
     gene            complement(7790..9598)
                     /locus_tag="VF516_22160"
     CDS             complement(7790..9598)
                     /locus_tag="VF516_22160"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8110460.1"
                     /translation="MASITATQEAGAAVRAPPEPQPAIQDHALIGDLHTAALVANDGS
                     IDFLCLPDFDSGSCFASLLGTPDSGRWNIAPVRPLTSVNRRYRGNTLILETEQAAETG
                     AVRLIDFMPIRRGAPRVVRIIEGLHGHVTLNFEMHPRFAYGFTLPRESSRGGTSAAIA
                     GPDALYLRGGRRGAPPFDGEVTVHAGERAAYELAWARPYHDVPPPLDIEAALRETEAF
                     WNQWTSRLRLPNFCPELVMRSLITLKALTYRPNGAIVAAPTFGLPEAPGGERNWDYRY
                     TWVRDSVLALQALMLAGLESEAEHFGEWLANAIGGAPGQFQIMYGIRGERILAELELP
                     WLAGHRDARPVRIGNAAYSQFQLDVVGEFAGVLLTFASMKGRFGEIEREAMRSAAEAI
                     AEVWQRDDHGIWEMRGPTRAFTTSKLSAWVTIDHWLKLIGQFDLDEDPHPWEALRDRI
                     RDEILEKGYDPKRNTFTQYYGSSNVDATLLFIPMSGFLPVSDPRVVGTVKAIEDELIE
                     DGLVLRYRTDASDDGLTGKEGVFLACSFWLASVYHMMGRRDDARRQFERAASVANDVG
                     LLAEEYLTNEKQQTGNFPQAFSHFALVNAAYQLGYP"
     gene            10382..11302
                     /locus_tag="VF516_22165"
     CDS             10382..11302
                     /locus_tag="VF516_22165"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8110461.1"
                     /translation="MLAIAGAAGVAADRSGLPAGVQRLLALVETTPAQRTPTRQQRAS
                     LLARIGTRALARRDVAAEVLAAIGALTVALGRFVVGRAKFRRSDLWLQMRACGVSALG
                     IVSLVAGLVGLIIAFISAEELKSFGASLYVANLVGIAIVRELGAVMSAIVVAGRTGAA
                     FAAELGTMQVTQEIDALTTLGLSPVEFLVVPRVLAVTLMLPLLCVYADVVGVLGGAVV
                     GIGVMHVAPRLYFDQTIHAVSLTDLFGGLVKAATYGFLIGSVGCFMGLRAGRSAAAVG
                     RAATSAVVAGIVLVIAACGLYSLVFYRFGL"
     gene            11305..12069
                     /locus_tag="VF516_22170"
     CDS             11305..12069
                     /locus_tag="VF516_22170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8110462.1"
                     /translation="MERSPHIAVRDLTVQYGDYLVLHDVSFTVRRGDVFVIMGGSGSG
                     KSTLLNAMIGLVEPATGEVMYDGKSFTHAEPHERQMILRRLGVLFQFGALWSELTLAE
                     NVALPLEELTSLGRREIRELAELKLALVGLGGFAEFYPNEISGGMRKRAGLARAMALD
                     PEILFFDEPSSGLDPINARRLDDLIIELRDSLNTTIVTVSPDLASIFATANDGVYLDA
                     QHKTVTAAGDPRGLLAHPPNAHIRAFLTRTDDKEAT"
     gene            12066..>12573
                     /locus_tag="VF516_22175"
     CDS             12066..>12573
                     /locus_tag="VF516_22175"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8110463.1"
                     /translation="MSKRASPANIGAFVVIALALLVTAILILGGGRLFRTTAAVIVYF
                     DDSVGGLRVGAPVKFRGIEVGTVKDVRINMAGVALDPHHVLIPVVLEIDERQLRERGA
                     PALDLRDPKQVQRLVDQGLRAELALESFVTGVRSVELDIRPGTPARLVHARTYPEIPP
                     VQSPTQAIP"
BASE COUNT         1944 a         4315 c         4321 g         1993 t
ORIGIN      
        1 tcgctcaact acctgctgag ctcgcacgtc tggcggcagg accacaacgg cttcagccac
       61 caggatcccg gattcatcga ccacgtggtc aacaagaagg ccgaggtgat ccgggtctac
      121 ctgccgcccg acgggaacac gctgctggtg gtgaccgatc actgcctccg gagccgggac
      181 tgcgtgaacg tcatcgtcgc cgggaagcag ccggcaccgc agtggctgac catggacgag
      241 gcgatcaagc actgccagtc cgggatcggg atctggagct gggccagcaa cgacgagagc
      301 gacgagcccg acgtggtgat ggcgtgctgt ggcgatgttc cgaccctgga gaccctggcg
      361 acggtctcga tgatccggct ccatgccccg cagctcaagg tccgcgtcat caacgtcgtc
      421 gacctgatgc ggctgcagtc ctcggacgag cacccgcacg gcctctccga ccacgagttc
      481 gacgcgctgt tcacgaagga caaaccgatc atcttcgcct tccacggcta cccgtcgctg
      541 atccaccagc tcacctaccg gcgctccaat cacaggaact tccacgtgca cggttaccgc
      601 gaggagggga cgaccaccac gccgttcgac atggtggtgc tgaacaggct cgaccgcttc
      661 cacctcgcgc tcgacgtgat cgaccggctg ccgcagctcg ggtcgcgcgc cgcgtacttc
      721 cggcaggcga tccgcgatgc tctgatcgat cacaagcagt acatccagga gcacggcgag
      781 gacatgccgg agatcaccgg gtggcgctgg gagcgcggcg ccacgccgga gagcccgcgc
      841 ggcgtgagcc gcggcgcggc gacgtccacc gagggagaca acgtatgacc gtggctccgg
      901 atcgccggga ggcgccgttc cgtgcggccc agggcagcat cgcctggcag gtcgaggccg
      961 aagggttcga tcccgcccgc gagcacgcga tcgagagcat gttcgccgtc ggcaacggct
     1021 acctgggcgt gcggcacgtc ctgccgctgc ccggcgcgtg gggcgacctg ttcgtcgccg
     1081 gcgtgtacga tcgcaaggac gaggggctcg cgtactccga gctcgagttc ctggcaccgg
     1141 agcgcggaga cagcgtgcac gtgcagctcg agcctctgcc ctatccgttg cacgcctcgg
     1201 tccgggtcga aggcgagctg ctcgcgattc ccggggcgaa cgcgcgcgcg atgcggcggc
     1261 gcctcgatct ccggtgcgcc gtggtcgagg tcgaaggcgt ccacgagacc gagggggggc
     1321 ggcgcaccag gctgtcgtcg cttcactgcg cctcgcttgc cgatccgcac gtgctgcttc
     1381 agcaggtgac cgcggccgca gagaatcact gggggacgat cgagatcacg agctcgctgt
     1441 tcgaccccgt ggaggcgcac cgcgatcctc cagtcgtggt cctcgagcac atccgctccg
     1501 gcgatctcga gctgatccgc tacgccaccc acgcgtccgg ctacgaggtc tgtatcgcgg
     1561 cgcgggcatt cacgcgagaa gatggccgcg acgcggtcgt ctaccgccgc gtgctcgcgg
     1621 tgttcaccaa ccgggacgtg caggaccccc gggcggcagc gctcgcgcgc atcgagcagc
     1681 tcgccgccga tgcgttcgac gcgctgctgg gcgaccacga gcgcgcatgg cgcgagttct
     1741 gggaggatgc cgacatccgt gtcgatggca gcccggccac cgagcaggca cttcggttca
     1801 ccatgtatca cctgcggatc gcggtggacc gcgatccggg ggcctcgatc ccggggcgcg
     1861 cgttcacggg gcgcgcctat gagggccacg tgttctggga caccgagatc ttcatgttgc
     1921 cgttctacct gtacaccgcg ccgccgctgg ccaggaacct gctgcgctac cgccgtcaca
     1981 cgctcgacgg tgcgcgtgcg cgcgcgcgtt ccctcggcta cgcgggggcg tgcttcgcgt
     2041 gggaatcgac ggtcaccggc gaggacgtca cgccgacccg gatcatcttg aagacgacca
     2101 ggcaggagat cccgatcttc acgggaaccc agcaggtgca cgtcacggcg gacatcgcct
     2161 atgcggtctg gcgctactgg gaagcgaccc acgacgaggc gttcctggcc ggtgaaggcg
     2221 cggacctgct gttcgagacc gcgcggttct gggccagccg cgtgacgcgc gacgagcgcg
     2281 ggtaccacat ccgccaggtc gtcggcccgg atgagtatca ccacgacgtc gacgacaacg
     2341 cctacaccaa ctggatggcg cgcttcaacc tggagcgcgc ggcgtggctc gccggatcgc
     2401 tgggatcggc cgctcgcccg cggctcggcg ccgacgagcc gtcccgctgg caggaggtcg
     2461 ccgccggcct gtgctgcgcc ggaccggatg accgcggtgt gatcgagcag ttcgcggggt
     2521 tccacgcgct ggaagactac cggctctcgc cgcaggagcg gttcaaggct ccggtgagcc
     2581 gcctgttcca ctgggacaag gtcaaccggc tcaagctcat caagcagccc gacgtcctca
     2641 tgctgctgat gctgtttcca gaggcgttcc cacgcgacgt cgtggcagcg aactatcgct
     2701 actacgagcc gctcaccgat cacggaagca gcctgtcggc gccggtgcac gccgcgaccg
     2761 ccgtgcgcct cgggctctac gaggacgccg agcgctactg gaagcaaagc ctgtggttcg
     2821 atctgtcgaa ccggatgcaa aacagcgatc tcggcattca ccccgctgcc atgggcggtg
     2881 tgtggcaggc gctcgtgttc ggctggctcg gcgtgcggtt caccagcgac ggaccgcagg
     2941 tggatgctgc ccccgcggtc cggctgccgg cgcaatggcg cgccgtcgcg ctgaagctcg
     3001 cgtggcgcgg tcgaacctac ccggtggcgc gatccgcgga gatgccagga gggccaccat
     3061 gagctcgttc gactcgatcc tgctcccgct cgatggctcg ctgcaggcgg cgaaggccgt
     3121 gccgagcgcc ctctggctcg cagagcggct gcacgcaacg ctgcacgtgt tgcatgctgc
     3181 cccacatccg ctcgcgcctg cgaacgccct ggagcggttg tccgcgctgg cggcacagcg
     3241 ctcgcggacc gtgctgcacc agacgaaggt cgaccccgtg caagcagtgc tcgaggcgac
     3301 caagcagcac cacgtgaagc tcgtcgtcat caccgcccgc ggcgaatcgg cgagccgaga
     3361 cgtcgagcgc ggccggactc tcgggcggat cgcgcgcgcg ctggttgtcg agagcagcgt
     3421 gcccgtcctg ctcgtaccgc ttcatgacag gagcgtgctg ccgtggcggt cgatgctggt
     3481 cgcggcaagc ggcgatcccg aatccgacgg ggcgctcgcg gccgcagccc ggctggcctc
     3541 ggcgctggac cttcgcgtga ccgtggtcca cgtgatcgcc gaaccgggct ccggcgggct
     3601 cggggggtat gccgacgccg cataccacga gcttccgtgg cgtctcgagg agatggtcaa
     3661 gcgggcgctc ctgagctcgt cacccgagga cgtcggccgc atcgaagggc tggccgtccg
     3721 tcgcggcgac ccgatcaccg aggtcatcgc cgaggtcgag caacgtcgaa gcagcgtggt
     3781 cgccgtcggg tggcatggag atctcagccc cgagcacgcc aacgtgctcc tcggcctcct
     3841 ggaacgggcg gccttcccac tcctggtcgt ccggaagccg cctccaccgg agtttcgcct
     3901 gaaggtcgga gacgagatcg gaccgcggcc ggattccacc gagggagacc acgtatgatc
     3961 gcggctgcgc gaagcagcga tcggagcgcg gcgtgagggt cgtgctggtg aacgccggct
     4021 cgagcagcct gaagtgctcg ctgatggagt cggcggaccg cacggtgctc gcgcacgtcc
     4081 aggccgactg ggcggggccg gtcacgcgct acgagcggtc cggaccgggt ggggcgcgga
     4141 tccgcgagca ggccgggttc cgcgagcacg gcgaggcggt gcggcatgcg ctcgacgatc
     4201 tccgcgcagc gtcggcgccg accgccgagc gaccggcggc cgtcgggcat cgcatcgtgc
     4261 acggcggcga attcagcgca ccggtgcgga tcacacccgc ggtccgctcg cggatctccg
     4321 cgctcgccga gctcgcgccg ctgcacaatc cgcccgggct gaacgcgctc gcggcggccg
     4381 aggcgctgct accggacatc ccgcacatcg ctgtgttcga cactgcgttt cacaccacac
     4441 tgccgcccgc ggcgcggacg tacccgctgc ctcacgcgtg gaccgagagc tggggcatcc
     4501 gccgctacgg atttcacggc ctcaacgctt cctactgcac gacccgcgcc gcggagctgc
     4561 tcgggcgccc ggcctccgag ctgcgcctgg tgatctgcca cctcggccac ggctgttcgg
     4621 ccaccgcggt gctccacggc cactcggtcg acacgacgat gggcttcacg ccgctcgatg
     4681 ggctgatgat ggcgacccgc agtggctcgg tggaccccgg aatctcggcc cacgtgcagc
     4741 gctgccacgg cctcggcgcc gaggagatcg acgaagcgct gaatcacacc tccggcctgt
     4801 tcggcgtatc cgggctctcg ggcgacatgc gggaggtgct ggctgcgggc cgcgccggcc
     4861 acgagcgcgc gcggctggcc atcgcgatct acgtgcaccg cgtgcggcag gcgatcggtg
     4921 ccttggcggc gacgctcggc ggcgtcgatg tgctggtgtt caccgccggg gtcggagaga
     4981 actcggcaga ggtccgcatg gcgagctgcc agggtcttga acacctgggg atcgagctcg
     5041 atgcagatgc caacgcccgc tgtcaccccg acgccgacgt ggcgggttgg acggcgcacg
     5101 cgcgcgtcct cgtgatcacc gcgcgcgaag atctcgcgat gctctcggac gtggtccggg
     5161 tcatcgcaac tgccaaggag ttaaacggat gatggacctc acacaaacca ccgctgactt
     5221 gacccagccg ggcaagggaa ttctcgcggc ggacgagacc gtgtcgacgc tgacgaagcg
     5281 gttcgatgcg ctggacatcg cgtcgacgtc gtcgagccgg cgcgactacc gggagatgct
     5341 gctcaccacc tcgggcgtca gggagttcat ctcgggcgcg atcctgcagg acgagacgat
     5401 ccaccagcac agctccacgg gcgcgccgat cgccgcgttg tgcagccagc tcgggatgct
     5461 gcccggcatc aaggtcgatg ctggcaccag gccgcttgcc gcgcacccgg gagagcaagt
     5521 gaccgagggc ctcgacggcc ttcgcgaccg gctggtcgag taccggggcc tcggtgcgcg
     5581 cttcgccaaa tggcgcgcag tgttcgcggt gaccgacacg ctgcccagct cggcatgcac
     5641 gtcggcaaac gccggggcgc tggctcgcta cgcggcgctc tgtcaggaac aggagctggt
     5701 gccgatcgtc gaacccgagg tgttgatgga cggagcgcac accatcgagc gctgcgagga
     5761 agtcacgggc tgcgtgctgc atgccgtgtt cgaggcgctc gtcgagcaag gggtccgcct
     5821 cgaggagatg ctgctcaagc cgaacatggt cgtgccgggc gacgacagcc cgcaacaggc
     5881 cgatgccggc acggtggccg ctgcgacgct gcgctgcctg cgccgccacg tgccggccgc
     5941 agtcccggga gtggtgttcc tgtccggtgg ccagcgcgat gtggacgcga cccgacacat
     6001 ggacgcgatc aaccgggccg gcgggcccaa gccctgggcg ctgagcttct cgtacggtcg
     6061 cgcgctccag gacgccgccc tcgcggcgtg gcgcggccgg ccggagaacc gggtgcctgc
     6121 ccagacaaaa ttttacaacc gcgcgcggtg caacgggctg gccgcgctgg ggcggtatga
     6181 cgagcgcgag gacagccagc aggcgcgggg gcccgagccg cgtgcctcgg caggctggca
     6241 ggacgactga aggagtgcgc gatggacaag gaccgaccca tctctgcggt gctgaccgac
     6301 gtggacggca cgcttgtcac caaggacaag gtgctgaccg agcgcgcgct cggcgccgtg
     6361 aaacagctgc gtgatcgcgg catcgtgttc acgatcacga gcggtcgccc tccgttcggc
     6421 atgcggatgc tggtcgagcc gctcgggctg accacgccga tggcggcgtt caacggtggg
     6481 gcgatcgtga gcccggacct gtcggtgctc gacgagcgac ccctgccgga tgctctgctg
     6541 ccggcactgg tcgacatgat ccaggcgcac ggtctcgacg tgttcctgtt ccggaccgat
     6601 gactggttcg tgcgctcgct cgacgctccc cgggtgcacc gcgaggcatc gaccatccag
     6661 cggctcccga ccgtcgtccc gagcttcgat tccgtgctgg cgggtgtggt gaaggttgtc
     6721 ggggtcagcg acgatcatcc ccgcgtcgcc gcctgcgagg ccgcggtcca gcaggcgtac
     6781 ggggcgcagg tctcggccgc gcgctcgcag ccctactacc tcgacgtgac caatccgacg
     6841 gcgaacaagg gcgtggtcat cgagcgtctg tcgcactaca tcgcgatccc gctcgagcgc
     6901 atcgccgcga tcggcgatca gccgaacgac gtgctgatgt tcgagcgcgc cgggctcagc
     6961 atcgcgatgg gcaacgcgag cgacgaggtc aagcgcaggg ccacgttcgt gaccacctcg
     7021 ttcgcggacg aggggttcgc gaacgcgatc gagcagatcg tcctgccgcg ggccgaaccc
     7081 gcgggggccc gcgatcagtg gtagccggcc tcgcggtgct cggtggaccc agcgacggcg
     7141 cgtgcctcgc cttgccagag ccggaagcct ccctccaacg cgaacgcgtt gtctccggcc
     7201 tcgcagccgg gcggtagccg cttcagcttc atcgcattac cgccgccgat cacgacgcga
     7261 tcgggatgaa aggcggccat cagctgctcg acgacgatct cgagcgtctt ctgccacttc
     7321 ttcctgccga tacgcttcag gcccgcgctg ccgaggtagt gctcgatggt cttcttgcga
     7381 tacggcagat gaccgatctc catcggcacg ctcgtcccgt cgacgatcag gcatgcgccg
     7441 acgccggtgc cgaggccgat gaacaagagc gcccctccct cgtagcagcc gagcgcctgc
     7501 atcgcaccgt cgttgatcag cttgaccggg cgaccgaagg cgcgctggaa gtcgtagccg
     7561 acccaaccct ttccgatgtt gtagggctcg gcgacgatcc ggccgccgcg aatctggccc
     7621 ggatagccga tcgacaccac gtcgtactcc cagtcgttca cgagctcgag cacaccggcg
     7681 accatcttgt ccggcgtcag cttcggcccc gacgggaact tcctgggctc gagcttgccg
     7741 ctcacgagca tctttgcgtt cgtgccgccg atatcgatcg cgaggatatt cacgggtagc
     7801 ctagctggta ggccgcattc accagcgcga agtgactgaa cgcctgcggg aagtttccgg
     7861 tctgctgctt ctcgttggtc aggtactctt cggcgagtag accaacatcg ttcgccacgc
     7921 tcgccgcacg ctcgaactgc ctgcgcgcat catcgcggcg gcccatcatg tgatagacgc
     7981 tcgccagcca gaacgagcac gcgaggaaca cgccttcctt gcccgtcagg ccgtcgtcgc
     8041 tcgcgtcggt gcggtagcgc agcacgagcc cgtcctcgat cagctcgtcc tcgattgcct
     8101 tcacggtgcc gaccactctg gggtcgctca ccggcagaaa tccgctcatc gggatgaaca
     8161 gcagggtcgc gtcgacgttg ctcgatccgt agtactgcgt gaacgtgttg cgcttggggt
     8221 catagccctt ttcaaggatc tcgtcgcgga tccggtcgcg cagcgcctcc cacgggtgag
     8281 gatcctcgtc gagatcgaat tgaccgatca gcttgagcca gtgatcgatc gtcacccacg
     8341 cgctcagctt cgaggtggtg aacgcgcgcg tcgggccgcg catctcccag atgccgtggt
     8401 cgtcgcgctg ccacacctcg gcgatggcct ccgcggccga gcgcatcgcc tcacgttcga
     8461 tctcaccgaa ccggcccttc atgctggcga acgtgagcag aactccggcg aactcgccga
     8521 ccacgtcgag ctggaactgg ctataggcgg cattgccgat gcgaacgggg cgcgcgtcgc
     8581 ggtggccagc cagccagggc agctcgagct cggcgaggat ccgttcaccg cggatgccgt
     8641 acatgatctg gaactgcccc ggtgcaccgc cgatcgcgtt cgccagccac tcgccgaagt
     8701 gctcggcctc gctctccagt cccgccagca tcagcgcctg gagcgccagc acggagtcac
     8761 gcacccaggt gtatcgataa tcccagttgc gctcgccgcc cggcgcctcg ggcaaaccga
     8821 aggtcggcgc cgcaacgatc gcgccgttcg ggcgatacgt caatgccttg agcgtgatca
     8881 gcgatcgcat gaccagctcg gggcaaaagt tcggcaggcg gaggcgcgac gtccactggt
     8941 tccagaacgc ctcggtctcg cgcaacgccg cctcgatgtc gagcggcggc ggcacgtcgt
     9001 gatagggccg cgcccacgcg agctcgtatg cggcgcgctc gccggcatgg accgtgacct
     9061 caccgtcgaa cggcggtgca ccacgccgcc cgccgcgcaa gtagagtgca tccgggccgg
     9121 cgatcgcggc cgaggtgccg ccgcgcgagc tctcgcgtgg cagcgtgaac ccgtaggcga
     9181 agcgggggtg catctcgaaa ttcagcgtga cgtggccgtg cagcccctcg atgatgcgga
     9241 cgacccgagg cgcgccccgc cggatcggca tgaagtcgat cagccggacc gcgccggttt
     9301 ccgccgcctg ctcggtctcg aggatcagcg tgttgccgcg gtagcggcgg ttcaccgacg
     9361 tcagcgggcg aaccggcgcg atgttccagc gtccgctatc gggtgtgccg agcagcgacg
     9421 cgaaacacga gccgctgtcg aaatcaggaa ggcacaagaa gtcgatcgag ccgtcgttcg
     9481 cgacaagcgc agcggtgtgc agatcgccga tcagcgcgtg atcctggatc gcgggctgcg
     9541 gttctggtgg ggcccgcacc gcagcaccgg cttcctgggt ggccgtgata gatgccatga
     9601 ccgagtctgg atcccacgcg acttcacgca atggcgtgct cgcgggtatg cgccagcgca
     9661 cgccgtgcac accgcacacc atagagcgcg tgctggcggc agcgcgactt gtacaggttg
     9721 atgagtccgt ttcgtcgagc tgtcgtgact cgtcacccgc gccgcgccgg cgagctcggg
     9781 caggattctt cgacgcgagc tgtttgccga gctcgatgcc ccactgatca ccagcacgat
     9841 gcgcacgccc agatccgccg cgcgcagcga ccaagacgcc agctggtcct gcgccgatca
     9901 ccaggatgtc tgtcatcctc ggtggctccg tgaggcagga ggcgttgcag ctctggttcc
     9961 gcggcgcctc ggggcgcgaa tcgctgaggc ctgcgtcgcc gtcgcacagc ccagcaccga
    10021 gctccgtgcg gtgctacgca catggatggg cagtggcagc tcgccgcgcg cgcgtcggtc
    10081 gacgtgatcg acgccaacta cgatgacgcc acctctcgtg ccggggaatg attgctgcag
    10141 aggttctctt cgagccatca tgcagtcccg tgcgagcgcc gatgtaccgc agctgaagat
    10201 gacgcgtcct gcgcgtggcg ccctgcagct cgagctaggc gggatctgga gggtcggcga
    10261 gccgctcccc gcgccggcgc tcgtcggacg agagatcgat gccgagcgtc cggatcacct
    10321 gcgcttcgac acgcgaggtg tgcaggcatg ggactccgcg cttgtcgcgt tcgcgacccg
    10381 cgtgctggcg atcgcaggcg cggcaggggt cgccgccgat cgcagcggcc tgccggccgg
    10441 ggtccagcgc ctgctcgccc tcgtcgaaac aacccctgcc cagcggacac ccacgcggca
    10501 gcagcgggca tcgctcctcg cgcgcatcgg aacgcgcgcg ctggctcgcc gcgatgtagc
    10561 ggccgaggtc ctggccgcga tcggcgcgct gacggtcgca ctcggaaggt tcgtggtcgg
    10621 acgagcgaag ttccggcgca gcgacctgtg gctgcagatg cgcgcctgcg gcgtcagcgc
    10681 gctcgggatc gtcagtctcg tcgccggact ggtagggctg atcatcgcgt tcatctccgc
    10741 cgaagagctc aagtcattcg gtgcctccct gtacgtcgca aacctggttg gcatcgccat
    10801 cgtgcgcgag ctcggtgccg tgatgtctgc gatcgtcgtc gccggccgga ccggtgcagc
    10861 ctttgccgcc gagctcggta cgatgcaggt cacacaggag atcgacgcgc tgacgacgct
    10921 ggggctctcg ccggtggagt tcctcgtggt gccgcgcgtc ctcgcggtga ccctgatgct
    10981 accgctgctg tgcgtctacg ccgatgtggt cggcgtgctc ggcggcgcgg tggtgggcat
    11041 cggcgtcatg cacgtcgcgc cgcggctgta cttcgatcag acgatccatg cggtgtcgct
    11101 caccgatctg ttcggaggcc tcgtcaaggc ggcgacctac ggttttctga tcggctcggt
    11161 cggctgtttc atgggactgc gcgcgggacg gagcgcggcc gcggtcggca gggccgccac
    11221 atcggccgtg gttgcaggca tcgtgctcgt gatcgcggcc tgcgggctgt actcgctggt
    11281 gttctatcga ttcggtctct agccatggag cgcagtcctc atatcgcggt tcgcgatctc
    11341 accgtgcagt acggggacta cctggtgcta cacgacgtga gcttcaccgt gcgccgaggc
    11401 gacgtgttcg tgatcatggg cggcagcggc agcggcaaga gcaccctgct gaacgcgatg
    11461 atcgggctcg tggagcccgc gaccggagag gtcatgtacg acggcaagag cttcacccac
    11521 gccgaaccac acgaacgcca gatgatcctg cgtcggctcg gcgtgctgtt ccagttcggt
    11581 gcgctgtgga gcgagttgac gctggccgag aacgtcgcgc tgccgctcga agagctcacg
    11641 agcctcggcc ggcgcgagat ccgcgagctg gccgagctca agctcgcgct cgtcgggctc
    11701 ggcggttttg ccgagttcta ccccaacgag atcagcggcg gcatgcggaa gcgagccggc
    11761 ctggcgcgcg cgatggcgct cgatcccgag atcctgttct tcgacgagcc gtcctcgggg
    11821 ctcgatccga tcaacgcgcg ccgtctcgac gatctcatca tcgagctgcg ggacagcctg
    11881 aacaccacga tcgtgaccgt gagccccgat ctcgcaagca tcttcgcgac cgcgaacgat
    11941 ggcgtctacc tggatgcgca gcacaagacg gtgaccgcgg cgggggatcc gcggggcctg
    12001 ctggcgcacc cgccgaatgc acacatccgg gcattcctga cccgcaccga cgacaaggag
    12061 gcgacatgag caagcgcgca tcgccggcca acatcggggc gttcgtggtc atcgcgctgg
    12121 cgctgctggt gaccgcgatc ctgatcctcg gtggaggccg actgtttcga accaccgcgg
    12181 cggtgatcgt ctatttcgac gactccgtcg gggggctgcg ggtcggcgcg ccggtgaagt
    12241 tccgcggcat cgaggtcggc acggtcaagg atgtgcgcat caacatggcc ggggtcgccc
    12301 tcgatccgca ccacgtgctg atcccggtgg tgctcgagat cgacgagcgc cagctgcgcg
    12361 aacgcggggc ccccgcgctc gatctccgcg acccgaagca agtgcagcgg ctcgtcgacc
    12421 agggacttcg cgccgagctc gcgctcgaga gcttcgtcac cggggtccgc tccgtcgagc
    12481 tcgacatccg gccgggcaca ccggcgcgcc tggtccacgc tcgcacgtat cccgagattc
    12541 caccggtgca gagcccgacc caggcgatcc ccg
//
LOCUS       DASVOR010001162         4619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_12482,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001162 DASVOR010000000
VERSION     DASVOR010001162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4619
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12482"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1943
                     /locus_tag="VF516_22180"
     CDS             <1..1943
                     /locus_tag="VF516_22180"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8110464.1"
                     /translation="PAPPAPAAPAPATPGRTEAMTADTPRTTVEGARFVAPAGWSITV
                     RGPATILEAPEAGSRLALVDVHAADADAAVDQAWAAYRPDRPWKLEHKGKRADGDGWT
                     DTTQYAYQTSPDERRDVFAQAMRHDQTWTVAIYDMARAVGEKRGSQINVVFDGLLPKG
                     YQRESFAGKTAHRLDAARIAELTRFVDHARELFDVPAISFGLLQDGKAVFAGGFGVRE
                     LGKPQPPDADTLYIIASNTKALTTLLLAKLVDEHRLTWDTPVTRVLPSFKLGDEATTR
                     SVLVKHLICACTGLPRQDLEWLFDAHRLTPAGALALLGTVQPTSSFGALFQYSNLMAA
                     SAGFVAAYTISPGRELGAGYDAAMQAKVFGPLEMTSTTFDFRRALRANHAGAYGLDVD
                     GKPAPAVMDANYTVRPIRPTGGAWSNVRDMLRYVAMELARGVLPDGRRYIGEDALLAR
                     RAPQVAISKDESYGMGLMVDRTWGIPVVHHGGDLFGYHSDMMWLPDHGVGAVILTNAQ
                     AGGIIRELLRRKLLEVVFDGQPLADAQLAAAAKEVREQITANRPRLTVPPTEDAARLA
                     ARYTSAALGELDIARDHGAVVFDFGEWRSPVASRHNPDGSVSFVLIAPGLRMRELVAG
                     ERDGKRTLTIRDGQHEYVFVER"
     gene            complement(2088..2861)
                     /locus_tag="VF516_22185"
     CDS             complement(2088..2861)
                     /locus_tag="VF516_22185"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8110465.1"
                     /translation="MTAGMIVIMNQCMAAKSKRERKRHVQQQLFRHGGKRRGAGRKPK
                     GARAGERHATRSAFKSYHPLHVVMRILPAVGSLRRRAMYKAMRAASITAALREWFRIV
                     HISLQRTHVHMIVEAEDPAALARGMQGFSISAARHINTALGDRGKRRRGKVFADRYHL
                     EVITTPTRARHAISYVLSNWRKHGEDRDGLPSTWLVDPFSSGSSFPDWKELQDQAWMW
                     PIRETYDPLIVRRPTTWLLAEGWKLAGEISARDVPRGRR"
     gene            4021..>4619
                     /locus_tag="VF516_22190"
     CDS             4021..>4619
                     /locus_tag="VF516_22190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110466.1"
                     /translation="MADGSAVLVERDDARASLRLRSGEQVILVTCPAGTPPAALRPAF
                     DSAVQRAAAQVVHVIAVGGAPGVARELKRSAPFWQLRRRFGFHHVDASGSVERVTGQR
                     LSWLNQAVEIAAMVQAPGDEYFAPRLAQGQQVHGEENRLDAALSNRFPWMTVTLGAVC
                     VLLFVLGEIWSDGNFSLVLYRMGANSGEEVRAGEVWRLVA"
BASE COUNT          690 a         1637 c         1568 g          724 t
ORIGIN      
        1 cgccagcccc acctgcgccg gccgcaccgg cacccgccac ccccgggcgc accgaggcca
       61 tgaccgcgga cacgccgcgc acgacggtcg agggtgcgag gttcgtcgcg cccgccggct
      121 ggtcgatcac ggtgcgcgga ccggcgacga tcctcgaggc tcccgaggcg ggctcgcggc
      181 tcgcgctggt cgacgtccac gccgcggacg ccgacgcggc ggtcgaccag gcctgggccg
      241 cctacaggcc cgaccggcca tggaagctcg agcacaaggg caagcgggcg gatggcgatg
      301 gctggaccga caccacgcag tacgcctacc agacctcgcc cgacgagcgg cgcgacgtgt
      361 tcgcgcaggc gatgcgccac gatcagacct ggacggtcgc gatctacgac atggcgcgcg
      421 cggtcggcga gaagcgcggc agccagatca acgtggtgtt cgatgggcta ctgccgaagg
      481 gctaccagcg cgagtcgttc gccggcaaga ccgcgcaccg gctcgatgcc gcgcggatcg
      541 ccgagctgac ccggttcgtc gaccacgcgc gcgagctgtt cgacgtgccg gcgatctcgt
      601 tcggcctgct gcaagatggc aaggccgtgt tcgccggcgg gttcggggtc cgcgagctcg
      661 gcaagccgca gccgcccgac gccgacacgc tctacatcat cgcgtccaac accaaggcgc
      721 tgaccacgct gctgctcgcc aagctggtcg acgagcaccg gctgacctgg gacacgccgg
      781 tgacccgcgt gctgccgtcg ttcaagctcg gcgacgaggc gacgacccgc agcgtgctgg
      841 tcaagcacct gatctgcgcg tgcaccgggc tgccgcgcca ggacctggag tggctgttcg
      901 acgcgcaccg gctcacgccg gcgggcgcgc tggcgctgct cggcacggtg cagccgacca
      961 gctcgttcgg cgcgctgttc cagtactcga acctgatggc cgcatcggcc gggttcgtcg
     1021 ccgcgtacac gatctcaccc ggccgcgagc tcggcgccgg ctacgacgcc gcgatgcagg
     1081 ccaaggtgtt cggtccgctc gagatgacgt cgacgacgtt cgacttccgg cgcgcgctgc
     1141 gcgccaacca cgccggcgcg tacgggctcg acgtcgacgg caagccggcg cccgcggtga
     1201 tggacgccaa ctacaccgtg cgcccgatcc ggccgaccgg cggcgcgtgg agcaacgtgc
     1261 gcgacatgct caggtacgtc gcgatggagc tcgcgcgcgg cgtgctgccg gacggccggc
     1321 gctacatcgg cgaggacgcg ctgctcgccc gccgtgcccc gcaggtcgcg atcagcaagg
     1381 acgagagcta cggcatgggc ctgatggtcg atcgcacgtg gggcatcccg gtcgtccacc
     1441 acggcggcga cctgttcggc taccacagcg acatgatgtg gctacccgat cacggcgtcg
     1501 gcgcggtgat cctgaccaac gcccaggccg gcggcatcat ccgcgagctg ctccgccgca
     1561 agctgctcga ggtggtgttc gacggccagc cgctcgccga tgcccagctc gccgccgctg
     1621 cgaaggaggt gcgcgagcag atcaccgcga accgcccgcg cctgaccgtc ccaccgaccg
     1681 aggacgcagc ccggctcgcc gcccgctaca ccagcgccgc gctcggcgag ctcgacattg
     1741 cccgcgatca cggcgcggtc gtgttcgact tcggcgagtg gcgatcgccg gtcgcgtcgc
     1801 gccacaaccc ggacggatcg gtgtcgttcg tgctgatcgc cccggggctt cgcatgcgcg
     1861 agctggtcgc cggcgagcgc gacggcaagc gcacgctgac catccgcgac ggtcagcacg
     1921 agtacgtgtt cgtcgagcgc tgaggcccca tggcctatcg atcgcattga tggagatcgg
     1981 ccgcgatccc tgaccgctcg aacgctctgc gggctccgag cgctcgcaag gcgtccgcct
     2041 cggtcaccgc cgcctcggtc accgccgctc ggtcaccgcc gccccgatca tcgccgtccc
     2101 cgcggcacgt cgcgtgcgct gatctccccg gcgagcttcc agccctccgc cagcagccac
     2161 gtcgtcggtc tccggacgat cagcggatcg tacgtctcgc ggatcggcca catccacgcc
     2221 tggtcctgca gctccttcca gtcagggaat gagctgccgc tggagaaggg gtccacgagc
     2281 cacgtgctcg gcaagccatc gcggtcctcg ccgtgctttc tccagttgga caggacgtag
     2341 gaaatcgcgt ggcgtgcacg cgtcggcgtg gtgatcacct ccaggtgata ccgatcggcg
     2401 aacaccttgc cgcgccgccg cttcccccgg tcacccagcg cggtgttgat gtgtcgcgcc
     2461 gccgagatcg agaagccttg cataccgcgg gcaagcgccg ccgggtcctc ggcctcgacg
     2521 atcatgtgaa cgtgcgtgcg ctgcaagctg atgtgcacga tccggaacca ctcgcgcagc
     2581 gctgcggtga tcgacgctgc gcgcatcgcc ttgtacattg cgcgccgccg caggctgccc
     2641 accgcgggca ggatccgcat cacgacgtgc agcggatggt acgacttgaa cgctgaccgg
     2701 gtcgcgtgcc gctccccggc gcgcgcgccc ttcggcttcc gtcccgcgcc gcgcctcttg
     2761 ccgccatgcc ggaacagctg ctgctggacg tgtcgcttcc gctcgcgctt cgacttcgcc
     2821 gccatgcatt gattcattat cacgatcata ccggctgtca attgaatcgg cgcatctaat
     2881 cgtagtgatc gatcagggac ttgcatcgat cggggtgcat ggattgtgaa gtatagattg
     2941 tattacatct gttcccgaac ggcgacaggg tatcgggatc ggccgactgc cgatccggtt
     3001 tggatcgtcc cgctcagccg gatcctccga gctcgcggct gcagcctggc gaagaccgcc
     3061 ttgtgccgcc gcgcttaccg caggcgctgc gctcgaagga tcggctccga gcaatccctc
     3121 gacgaggacc gctgccgaac ctcgggctgc ctgtggacaa cgggtcggct tgatgacccg
     3181 cgccgcggca cgccagctcg aaccaggcgc ggctcacaag cctccttccg ttgcccacag
     3241 tccttgccct cgactccacg ccgcctggga gagcctattt gttgatctga gttcggataa
     3301 tgatattcag tctcgcgatg gatggaacag aacgagctgc gcaggggaag ccggatggcc
     3361 ggcccaggcc gtcgagttcc tcttctctcg aatccgaaca gaaaaatatc cgtgtgctgg
     3421 gagcatccgt gcggggcacc cgactgtgag taggcggtcg aggtcaacct tgtcgactta
     3481 ttccggctag tcacaggcga ccgggcgttc tcccgaggtc tgtccggctt cggtgagccg
     3541 gagcgaagct ccaggactgc gctgcggggt acaggagcgg gccgacaggt tgatatccct
     3601 atccgcgctc gagcccctcg gcggggtaat caccgatccg gtcgagcgcg atcttctcca
     3661 ttgactttgg ggtatcgcgc gcacgggtac caggccgcgc gccgcggagc cccggtcaca
     3721 cggtcgacat cactccgggg aacggccacg caacgtcgcg tggttgtgcg gcgctgtcgc
     3781 tgacgcggat cttcgtcaag ctcgcggcgg agaccggcga cgcgcacccg atctacgacg
     3841 cgcacttcaa cgacgcgtac aacaccgggt ctgcgctagg cgatcgagcc ccgacttcgt
     3901 gttgcgggtc gctcgcgccg cgcatagagt ccgcccatgg tggcaagctc ggctggggtt
     3961 cccggcggtg aggatctggc ggcggcaagg tcgcttcgcg accggatcct cgcagcgttc
     4021 gtggcggacg gatcggcggt cctggtcgag cgcgacgacg cgagagccag cctgcgcctg
     4081 cgctccggcg agcaggtcat cctggtcacc tgcccggccg gcacgccacc cgcggcgctg
     4141 cgcccggcgt tcgatagcgc cgtccagcgg gccgcggcgc aggtcgtgca cgtgatcgcc
     4201 gtcggcggtg cccctggggt cgcgcgcgag ctgaagcgga gcgctccgtt ctggcaattg
     4261 cggaggcgat tcggatttca ccacgtcgat gccagcggca gcgtcgagcg cgtcaccggg
     4321 cagcggctct cctggctgaa ccaggcggtc gagatcgccg ccatggtcca agctcccggc
     4381 gacgagtact tcgcgccgcg cctcgcgcag ggccagcagg tccatggcga ggagaaccgg
     4441 ctcgacgccg ccctgagcaa ccgcttcccc tggatgacgg tgacgctggg cgccgtctgc
     4501 gtgctcctgt tcgtgctcgg cgagatctgg tcggacggca acttctcgct cgtgctgtac
     4561 cgcatgggcg ccaacagcgg cgaggaggtc cgcgcgggag aggtgtggcg cctggtagc
//
LOCUS       DASVOR010001163         1619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_449953,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001163 DASVOR010000000
VERSION     DASVOR010001163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449953"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..328
                     /locus_tag="VF516_22195"
     CDS             131..328
                     /locus_tag="VF516_22195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110467.1"
                     /translation="MASDVRAVLASFDPVAYFPSMFAAYFRTLHQVIDALAPYLERAD
                     QPSWHALDSYYRADMWAFFED"
     gene            636..>1619
                     /locus_tag="VF516_22200"
     CDS             636..>1619
                     /locus_tag="VF516_22200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110468.1"
                     /translation="MTPRPAPRALPPRDLTSLQRAICARARDFEIAALLDLLATLGYR
                     PGDVTFCGHVAETPQPTLVHRIEFADLEGGLTGPGFGASVDLGSLTEIVAPPPGLLRP
                     PRRTGGRTSPVTVTVNLGLLSCRSPLPSYFQHLLRDTALQEPLAELLRIVDRNLLRAR
                     LTCDRPERIVEAWDDITSDLVRIHGLDSLIGLSWLFRHVFPELPAIVERTSDELRVPY
                     AAARLGFSALGSACLGASTRIDVHDFQVTLRAGESLLRPGVPWLHEVDRRLRTTVFPA
                     LEPVSMNLTIVLELADDRAFAMLRDDRPPPRESYIGMDPLGLPGAPDGPARR"
BASE COUNT          212 a          645 c          498 g          264 t
ORIGIN      
        1 cgaccctgat ccagtcggcg ccgccgacga ccccgtcgcc atcggatccc cggcgctggc
       61 ggtgctctag gccaagctgg ccggctttca ggcgctgatc gcgcgcggcg agctcgccag
      121 ggccgcggtg gtggccagcg acgtccgcgc cgtgctcgcc agcttcgatc cggtcgccta
      181 cttcccgtcg atgttcgcgg cctacttcag gacgctccac caggtcatcg acgcgctcgc
      241 accgtacctc gagcgcgccg accagccgtc gtggcacgcg ctcgactcct attaccgcgc
      301 cgacatgtgg gcgttcttcg aagactgaag atgtgtcgat caaagtgttc gatctgggtc
      361 ccccaccttg aggttgacct cgtcgttgac tccatcgtgg gccttgacgg cgacgtagga
      421 cgtcgccccc accgtcgacc tcgaccttgc ccatctcccg tcattctcga gcacgattcc
      481 tcgcaaatga tctggggtca cgtccaaggt cgacgacggg ctcaacgacc acgtcgccat
      541 caaggtcaac gccctgcgac aacggcaagg tcaacctcaa cggcctcgga ctcagcgcga
      601 gctgaatttc ctgcacatct cgagcccggc ctgccatgac gccgcgccca gccccccgcg
      661 ccttgccgcc gcgagacctg acctcgctcc agcgcgcgat ctgcgcgcgg gcccgcgact
      721 tcgagatcgc cgcgctgctc gatctcctgg ccaccctggg gtaccgcccg ggcgacgtca
      781 ccttttgcgg ccacgtcgcc gagaccccgc agcccacgct cgtgcatcgc atcgagttcg
      841 ccgatctcga gggcggactc accgggcctg gcttcggcgc gtcggtcgat ctcggcagcc
      901 tcaccgagat cgtggcaccg ccgcctggac tgctgcggcc cccccgccgg accggcggcc
      961 ggacgtcgcc ggtgacggtc accgtgaacc tcggcctcct gagctgccgc tcgccgctgc
     1021 cgtcgtactt ccagcacctc ttgcgcgaca ccgcgctcca ggagccgctc gccgagctgc
     1081 tgcggatcgt cgaccgcaac ctcttgcgcg cgcgcctgac ctgcgaccgc cccgagcgca
     1141 tcgtcgaggc ctgggacgac atcaccagcg atctcgtgcg catccacggc ctcgactcgc
     1201 tgatcggcct gtcctggctg ttccgccacg tgttccccga gctgccggcg atcgtcgagc
     1261 gcacgtccga cgagctccgc gtgccgtacg cggcggcgcg gctcgggttc tccgcgctgg
     1321 gcagcgcgtg cctcggcgcg tcgacccgga tcgacgtcca cgacttccag gtcacgctgc
     1381 gcgcaggcga gtccctgctc cgccccggcg tgccgtggct gcacgaggtc gaccggcggc
     1441 tgcgcaccac ggtcttcccc gcgctcgagc cggtgtccat gaacctgacc atcgtgctcg
     1501 agctcgccga cgatcgcgcg ttcgcgatgc tgcgcgacga ccgcccgccc ccgcgcgaga
     1561 gctacatcgg gatggatccg ctcggcctcc ccggcgcccc cgacggcccg gcgcgccgc
//
LOCUS       DASVOR010001164         1639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420225,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001164 DASVOR010000000
VERSION     DASVOR010001164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420225"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1275)
                     /locus_tag="VF516_22205"
     CDS             complement(<1..1275)
                     /locus_tag="VF516_22205"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8110469.1"
                     /translation="MNPPRETRSPNLDPARMSRVEQERDFYRKMLELGQAREIRPFLE
                     EALALIVQMTGARRGYVELMADPDAGREPAFWVAHGCQDADVAAIRAAFSRGVIAEAI
                     ATGRTIITVSALEDPRFQTSKSVQRNRIEAVICAPVGAVPPAGVVYLQDQVAKGLFTE
                     RDRDQVETFARHLAPFVDRLLLRRHRRTEDDPTQAYRRVLRADDVIGRSAALAAVLQQ
                     ASAAAPLDVTVLLTGASGTGKTQLARVIHHSSPRAAGPFVELNCAALPDTRLESELFG
                     ALPGAHSTAHRKVAGKIAAAQGGTLFLDEVGELTLSAQSKLLQLLQSREYYPLGASSP
                     VRADVRIIAATNMDLKAAAQRKEFREDLYYRLAVLPIRSPSLAERREDVLPLAEHFCA
                     LACKTHGLPSLELSVDARCAIEAAEWPGNVREL"
BASE COUNT          221 a          594 c          591 g          233 t
ORIGIN      
        1 cagctcgcgc acgttgcccg gccactccgc ggcctcgatc gcgcagcgcg cgtcgacgct
       61 gagctcgagg ctgggcagcc cgtgggtctt gcacgccagc gcgcagaagt gctcggccag
      121 cggcaggacg tcctcgcggc gctcggcgag cgacgggctg cggatcggca gcacggcgag
      181 ccggtagtac aggtcctcgc ggaactcctt gcgctgcgcc gcggccttga ggtccatgtt
      241 ggtcgccgcg atgatccgca cgtcggcgcg caccgggctg gacgcgccga gcgggtagta
      301 ctcgcgcgac tgcaggagct ggagcagctt ggactgggcg gacagcgtga gctcgccgac
      361 ctcgtcgagg aacagcgtgc cgccctgggc cgcggcgatc ttgccggcga ccttgcggtg
      421 cgcggtcgag tgcgcgccgg gcagcgcgcc gaacagctcg ctctcgagcc gcgtgtcggg
      481 cagcgccgcg cagttgagct cgacgaacgg gccggcggcg cgcgggctgc tgtggtggat
      541 cacccgggcg agctgggtct tgccggtgcc cgacgcgccg gtcaagagca cggtgacgtc
      601 gagcggcgcc gcggcggacg cctgctgcag caccgccgcg agcgccgcgc tgcgcccgat
      661 cacgtcgtcg gcgcgcagca cgcggcggta ggcctgggtc ggatcgtcct cggtccgccg
      721 gtggcggcgc agcagcaggc gatcgacgaa cggcgcgagg tggcgggcga aggtctcgac
      781 ctggtcgcgg tcgcgctcgg tgaacaggcc cttggcgacc tggtcctgca ggtagaccac
      841 gcccgccggc ggcaccgcgc cgaccggcgc gcagatcacc gcctcgatcc ggttgcgctg
      901 cacgctcttg ctggtctgga accgcggatc ctccagcgcc gacaccgtga tgatcgtgcg
      961 gccggtcgcg atcgcctcgg cgatcacgcc gcgcgagaac gccgcgcgga tcgcggcgac
     1021 gtcggcgtcc tggcagccgt gcgcgaccca gaacgccggc tcgcgcccgg catcggggtc
     1081 cgccatcagc tcgacgtagc cgcgccgcgc cccggtcatc tgcacgatca gcgcgagcgc
     1141 ctcctcgagg aacggccgga tctcccgggc ctgcccgagc tcgagcatct tgcggtagaa
     1201 atcgcgctcc tgctcgacgc gcgacatccg ggccggatcg aggttcggcg agcgagtctc
     1261 ccggggcgga ttcatctcgg gaagctactc tgaaccgcgc agcaacggga gcacgcttcc
     1321 ggcgcgcgct ggcggcgatg cagatcgcgg tgacccgcgc acacatcgcg cgatccgcgg
     1381 atgtccccgt accgatcacg gcgcatttcg atcccctccg attctgcttg accgcttgcc
     1441 acctctggtc ttaccaattg gccgattggc cttaccagtc aacggagcct tcacatggcc
     1501 ctgcccaagc cgacggaagt caccgcgatc gacgccgtgt tcgagacctt gctcaagaac
     1561 atcgtcggcg gtgcgtaccc gtccgggacg cggctgcccg ccgagcgcga gctcgcccgc
     1621 cagctcggcg cgtcgcggc
//
LOCUS       DASVOR010001165         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420230,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001165 DASVOR010000000
VERSION     DASVOR010001165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420230"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..665
                     /locus_tag="VF516_22210"
     CDS             <1..665
                     /locus_tag="VF516_22210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110470.1"
                     /translation="YVVHYVLAYRRALYPAPPASWRVLERAEHAGRVVLYPGGNGFYP
                     VAQVMGGGPVSGIPDDMAACWATVRRVRPQLGPRSYSIGLSEVIRRGDIDLCYRALPN
                     VLAFQADGRDVDWAAPDEGIADTTDAMWIPRGLPPDVAACARAYIAFALSAPVQDRWC
                     GLLAALPVHRDAHHRHAAFARPGMPDDPDTQDRVLYVPEDVKATHEPAWEATFESLIA
                     GP"
     gene            complement(673..>2179)
                     /locus_tag="VF516_22215"
     CDS             complement(673..>2179)
                     /locus_tag="VF516_22215"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEX8110471.1"
                     /translation="LPPALREAIDAMPGGATRYRKRYDHDDHHLEVEFERLSPEALAQ
                     VVAFLRRRRREVLARRTVAQIVDVLERAAVRWLDPAYPPRAAAIPEIAFITGFSPEMV
                     AHAIDEEQASSRGPHLNLVLRSELGDPEYLDGFRPNLRTGGLTRAHGPGLVGAIFSSN
                     IPALPHLEIMRSFLVKSACLGRVSAGEPIFLRCYAETLHELDPELASCLAIVYWERGD
                     DELETRFLSSIDYLVAYGGDAQIQRLMATRPAALDATWHGHRLGFTYVTREALAAGQD
                     VHRLARAIAYDFAIFDGFACLCPQVVLVEDGGDTAPAELARLLADAMARWARELPPRR
                     LDLAGAARKQSFAELCMMSDAIEVIAAPPDGSYLVTVEAMQRFEPSGGDRYLRVVPVG
                     GPADLDRLIAPLPRQHLQCTAIAAGGAAGRHHELRELLASWGVTRIVPPGIMGRPSMM
                     WHHDGVACLGRMVTWCDHELVVPELLLGRDAGALIAEVERDPSATLGAALR"
BASE COUNT          284 a          807 c          816 g          272 t
ORIGIN      
        1 tctacgtcgt ccactacgtg ctcgcgtacc gccgcgcgct gtacccggcg ccgcccgcgt
       61 cgtggcgggt gctggagcgc gcggagcacg ccgggcgcgt cgtgctgtat ccgggaggca
      121 acgggttcta tcccgtcgcg caggtgatgg gcggcgggcc ggtgtccggc atccccgacg
      181 acatggcggc gtgctgggcg acggtgcgcc gggtccggcc gcagctcggg ccgcgcagct
      241 acagcatcgg cctgtccgag gtgatccggc gcggcgacat cgacctgtgc taccgcgcgc
      301 tgcccaacgt gctcgcgttc caggccgacg gccgggacgt cgactgggcg gcgccggacg
      361 agggtatcgc cgacaccacc gacgcgatgt ggatcccgcg cggcctgccg cccgacgtcg
      421 ccgcgtgcgc ccgcgcctac atcgcgttcg cgctgtcggc gccggtccag gaccgctggt
      481 gcgggctgct cgccgcgctg ccggtccatc gcgatgccca ccaccgccac gccgcgttcg
      541 cgcggcccgg catgccggac gatcccgaca cgcaggaccg cgtgctgtac gtccccgagg
      601 acgtcaaggc gacccacgag ccggcgtggg aagcgacgtt cgagtcgctg atcgccggcc
      661 cgtagccgcc gttcagcgca gcgccgcgcc gagcgtcgcc gatggatcgc gctcgacctc
      721 ggcgatcagc gccccggcgt cgcggccgag caacagctcg gggacgacca gctcgtgatc
      781 gcaccaggtc accatgcggc ccaggcacgc gacgccgtcg tggtgccaca tcatcgacgg
      841 gcggcccatg atgcccggcg ggacgatgcg ggtgacgccc cacgacgcga gcagctcgcg
      901 gagctcgtgg tgccgcccgg ccgcgccgcc cgcggcgatc gccgtgcact gcaggtgctg
      961 gcggggcagc ggcgcgatca gccggtcgag gtccgccggt ccgccgaccg gcaccacgcg
     1021 caggtagcgg tcgccgcccg acggctcgaa ccgctgcatc gcctcgacgg tcaccaggta
     1081 cgagccgtcc ggcggggccg cgatcacctc gatcgcatcg ctcatcatgc acagctcggc
     1141 gaagctctgc ttgcgcgccg cccccgcgag gtcgagccgg cgcggcggca gctcgcgcgc
     1201 ccagcgcgcc atcgcgtcgg cgagcagccg cgccagctcc gccggtgcgg tgtccccgcc
     1261 gtcctcgacc agcaccacct gcgggcacag gcacgcgaag ccgtcgaaga tcgcgaagtc
     1321 gtaggcgatc gcgcgggcga gccggtggac gtcctggccg gcggcgagcg cctcgcgggt
     1381 cacgtaggtg aagccgagcc ggtggccgtg ccaggtggcg tcgagcgcgg ccggccgggt
     1441 cgccatcagg cgctggatct gggcgtcgcc gccgtacgcg accaggtagt cgatcgagct
     1501 caggaaccgg gtctccagct cgtcatcgcc gcgctcccag tagacgatcg ccaggcacga
     1561 cgcgagctcg ggatcgagct cgtgcagggt ctcggcgtag cagcgcagga agatcggctc
     1621 gcccgccgac acccgcccca ggcaggccga cttcaccagg aagctgcgca tgatctccag
     1681 gtggggcagg gcggggatgt tggagctgaa gatcgcgccg accagccccg ggccgtgcgc
     1741 gcgggtgagg cccccggtcc gcaggttggg gcggaagccg tcgaggtact cggggtcgcc
     1801 gagctcgctg cgcagcacca ggttcaggtg cggcccgcgc gagctcgcct gctcctcgtc
     1861 gatcgcgtgg gcgaccatct ccggcgagaa cccggtgatg aacgcgatct cggggatcgc
     1921 ggcggcgcgc ggcgggtacg ccggatcgag ccagcggacc gcggcgcgct ccagcacgtc
     1981 gacgatctgg gccacggtgc gccgggccag cacctcgcgc cggcgccggc ggaggaacgc
     2041 gaccacctgg gcgagcgcct cgggcgacag ccgctcgaac tcgacctcga ggtggtggtc
     2101 gtcgtgatcg tagcgcttgc ggtagcgcgt cgcgccgccg ggcatcgcgt cgatcgcctc
     2161 gcgcagcgcg ggcggcagc
//
LOCUS       DASVOR010001166         3116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_479443,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001166 DASVOR010000000
VERSION     DASVOR010001166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479443"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..405)
                     /locus_tag="VF516_22220"
     CDS             complement(31..405)
                     /locus_tag="VF516_22220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002489686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEX8110472.1"
                     /translation="MADTRSGWIAIGALNAALAVAAGAFAAHGLRDRLDARALEVFET
                     GARYHMYHALGLIAAGLVASSAARGAQIAGWIFQVGIVLFSGSLYALALTGVKGLGAI
                     TPLGGLAFLAGWLWLAWAAWRG"
     gene            complement(616..1263)
                     /locus_tag="VF516_22225"
     CDS             complement(616..1263)
                     /locus_tag="VF516_22225"
                     /inference="COORDINATES: protein motif:HMM:NF022751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HEX8110473.1"
                     /translation="MDEPVVQLDHPRFRYVWASIFTRRLAPDCMTHRCTMVATQREKL
                     DACCQYGCDVDLGERAAIEARADQIRAVLDPDVRDARWFSSEEEEDPDYPSGRVVRTE
                     VRVGGCIFLAHDRRGCAIHRAAVEGGWDFRGVKPAICRLFPLSYETDSIVIADEYPEY
                     SCAHVSGPSLYRITRDALADLFGEALIAALDAAEDRVLTAVAASEATTARRLPVV"
     gene            complement(1278..1781)
                     /locus_tag="VF516_22230"
     CDS             complement(1278..1781)
                     /locus_tag="VF516_22230"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX8110474.1"
                     /translation="MTRFEELAHKVDAFFARVAERHGADMQCATGCSDCCHVRLTITA
                     VEAAAIRAHVAGWPAEQRRNLANQGPSDRCAGLDSAGRCRIYAARPLVCRSHGVPIRL
                     RGALPVVQACHRNFTRTAPDPDCVLDQATLSATLYAVEAADAAATGADPGQRIDLAAL
                     LADLAAI"
     gene            1819..2727
                     /locus_tag="VF516_22235"
     CDS             1819..2727
                     /locus_tag="VF516_22235"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8110475.1"
                     /translation="MDLNDIVVFTKVVETKSFTGAAEQLGLPKSTVSRKLAQLEERLG
                     VRLVQRTTRKLALTDIGQAYYERCARIVADVAAAEQLVTDMQSTPRGRLRMTAPVDLS
                     MRYLGSIIADFLAIHSDINIELEASDRIVDLIEEGFDLAVRFGTLPESTLIARRLCSI
                     SAVMCASPAYLAKRGAPTTIEELDDHDRVLFTPSPRTQSWTMTHGDQIYEFGRPARFA
                     SNNVGACHEAAASGAGIALLTDFMVARDCERGVLTYVLPDWTGRAIEVNAVYPARQNL
                     PPRLSLFLDHLAKALSPPPWAIPAAT"
     gene            2823..>3116
                     /locus_tag="VF516_22240"
     CDS             2823..>3116
                     /locus_tag="VF516_22240"
                     /inference="COORDINATES: protein motif:HMM:NF042735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8110476.1"
                     /translation="MANIAELENKLNDAILAGKAMEAFEELYDDNVVMQENSEPEYRG
                     KDFNRKREQEFFQSVEAWHGGAVLASAVHGDVSFSEWTMDVSLKGVGRIKLDQV"
BASE COUNT          494 a         1089 c         1121 g          412 t
ORIGIN      
        1 cggccaggag gaccagcggc gcggcacggg tcagccgcgc caggcggccc acgccagcca
       61 cagccagccg gcgaggaacg ccagaccgcc gagcggcgtg atcgcgccga ggcccttcac
      121 gccggtcagc gcgagcgcgt agagcgatcc gctgaacagc acgatcccga cctggaagat
      181 ccagccggcg atctgcgcgc cgcgcgccgc cgagctcgcg acgaggccgg ccgcgatcag
      241 cccgagcgcg tggtacatgt gatagcgcgc gccggtctcg aacacctcga gcgcccgggc
      301 gtcgagccga tcgcggaggc catgcgccgc gaacgcgccc gccgcgacgg cgagcgccgc
      361 gttgagggcg ccgatcgcga tccagcccga gcgtgtgtcc gccatggcgc gactgtagcg
      421 ccaacgcagc gcccggcacc gatccgccgt gcgggaccgc cacgtcaagc tcaacgatgg
      481 ccgtcaacgt cacggtccag gtcgacgtcc atcggacgct gtcgaggaag gccgggaggt
      541 ctgcggatcg gcgatgtccg gtggacgcgg tctggacgac cgcgcagacc acacaccgtg
      601 ggaccctggc cgacgtcaga cgaccggcag ccggcgcgcg gtggtggcct cggatgcggc
      661 gaccgcggtg agcacccggt cctcggcggc atccagcgcg gcgatcagcg cctcgccgaa
      721 gaggtcagcg agcgcgtcgc gcgtgatccg gtacaggctg gggccgctga cgtgggcgca
      781 cgagtactcg gggtactcgt cggcgatcac gatcgagtcg gtctcgtacg acagcggaaa
      841 cagccggcag atcgcaggct tgacaccgcg gaagtcccag ccgccctcca ccgcggcgcg
      901 gtggatcgcg cagccgcgcc ggtcgtgggc caggaagatg cagccgccga cgcggacctc
      961 ggtgcggacg acgcgacccg acgggtagtc ggggtcctcc tcctcctcgg aggagaacca
     1021 gcgggcgtcg cggacgtcgg ggtcgagcac ggcgcggatc tggtcggcgc gggcctcgat
     1081 cgccgcgcgc tcgccgaggt cgacgtcgca gccgtactgg cagcaggcgt cgagcttctc
     1141 gcgctgggtc gcgaccatcg tgcagcgatg cgtcatgcag tccggggcga gccgccgggt
     1201 gaagatcgac gcccagacgt agcgaaaccg ggggtgatcc agctggacca cgggctcgtc
     1261 catggcgcgt aggtctatca aatcgcggcg agatcagcga gcagcgcggc caggtcgatg
     1321 cgctggccgg gatcggcacc ggtcgcggcg gcgtcggcgg cctcgaccgc gtacagcgtg
     1381 gccgacagcg tggcctggtc cagcacgcag tcgggatcgg gcgcggtccg cgtgaagttc
     1441 cggtggcatg cctgcacgac cggcagcgcg ccgcgcagcc ggatcgggac gccatgcgaa
     1501 cggcagacca ggggccgcgc cgcgtagatc ctgcagcgcc ccgcggaatc gagccccgcg
     1561 cagcggtcgg acggcccttg gttcgcgagg ttgcgtcgct gctcggccgg ccagccggcc
     1621 acgtgggccc ggatcgccgc cgcctcgacc gccgtgatcg tcagccggac gtggcagcag
     1681 tccgaacagc cggtcgcgca ctgcatgtcc gcgccgtggc gttcggccac ccgggcaaag
     1741 aatgcgtcga ccttgtgggc caattcctcg aaccgggtca ccgcaccagg gatagcacgt
     1801 ggttagaatc ccctcgccat ggatctcaac gacatcgtcg tgttcaccaa ggtcgtcgag
     1861 acgaagagct tcaccggcgc ggccgagcag ctcggtctac ccaagtcgac ggtgagccgc
     1921 aagctggcgc agctcgagga gcggctcggc gtccggctgg tccagcgcac cacgcgcaag
     1981 ctcgcgctga ccgacatcgg ccaggcgtac tacgagcgct gcgcgcggat cgtcgccgac
     2041 gtcgccgccg ccgagcagct ggtcaccgac atgcagtcga cgccgcgcgg ccggctgcgg
     2101 atgaccgcgc cggtcgatct gtcgatgcgc tacctcggat cgatcatcgc cgacttcctg
     2161 gcgatccact ccgacatcaa catcgagctc gaggccagcg accggatcgt cgatctgatc
     2221 gaggagggct tcgacctcgc ggtccggttc ggcacgctgc ccgagtcgac cctgatcgcg
     2281 cgccggctgt gctcgatcag cgcggtgatg tgcgcgtcgc cggcctacct cgccaagcgc
     2341 ggcgcgccga ccacgatcga ggagctcgac gatcacgacc gcgtgctgtt cacgccgagc
     2401 ccgcggaccc agagctggac gatgacccac ggcgatcaga tctacgagtt cgggcggccg
     2461 gcgcggttcg ccagcaacaa cgtcggcgcg tgccacgagg cggcggcgtc cggcgccggc
     2521 atcgcgctgc tcaccgactt catggtcgcg cgcgactgcg agcgcggcgt cctgacctac
     2581 gtgctgcccg actggaccgg ccgcgcgatc gaggtcaacg cggtgtaccc ggcgcgtcag
     2641 aatcttccgc cccggctgtc gctgttcctc gaccatcttg cgaaggcgct gtcgccgccg
     2701 ccgtgggcga tccccgcagc gacgtaggcg gcacggtgcg aggcggtcac cgtgccgcca
     2761 gtggaacatc ctgtctcgcc ggtccggctt gccggggcgc cctacccacg gttagctggt
     2821 gcatggccaa catcgcagag ctggagaaca agctgaacga cgccatcctc gccggaaagg
     2881 ccatggaggc gttcgaggag ctgtacgacg acaacgtcgt gatgcaggag aactccgagc
     2941 cggagtaccg cggcaaggac ttcaaccgca agcgcgagca ggagttcttc cagagcgtcg
     3001 aggcctggca cggcggcgcg gtgctggcgt cagcggtcca cggcgatgtg tcgttctcgg
     3061 agtggacgat ggacgtctcg ctcaagggcg tcggccggat caagctggat caggtc
//
LOCUS       DASVOR010001167         3312 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_41165,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001167 DASVOR010000000
VERSION     DASVOR010001167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3312)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3312)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3312
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41165"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VF516_22245"
     CDS             complement(<1..419)
                     /locus_tag="VF516_22245"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8110477.1"
                     /translation="MTVLRSYTGGQWRSPAGEGTPLHDAVTGEEVARISSDGIDMAAA
                     LEYGRTVGGPALREMTFHQRAALLKALGSYLREHRDDLYALSGRTGATLADSKFDVDG
                     GIGVLASYASRGRRALPNDTVYLDGDVEPLGRGGGFVG"
     gene            complement(481..1680)
                     /locus_tag="VF516_22250"
     CDS             complement(481..1680)
                     /locus_tag="VF516_22250"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8110478.1"
                     /translation="MKALVFRHNLSREAAATIGGRIDRRAFVSRLAPVKVEDIDEPPL
                     PAQDWVRVNTTWSGLCGSDVKQIVLNGRRDNPLTALVSFPHVLGHEAVGRISETGQRV
                     VLNPWLSCGPRGIDPPCPACRNGRYPWCRNFRAGNLPVSIHIGNCAAAAGAHAERFAA
                     HTSQLFAIPDNISDDAAVLADPVSVSLRTILLAPPSGRAPVLVYGSGTLAFAAIALLR
                     HLYPGTEVWAATRAGSRAALATRLGADAVLPAAPEELIAEVARRVHTTPLEPWSKRPW
                     LQDGPAVIYDTIGSAETVETSLRLADTGGTLVVSGVEPPQRFEWTPLYFKELHVIGSN
                     GFGIETVSGVAKHSMEHYFDFIAAGFDLTPVITHRFRLERWQEAVLTLKNARHTGAVK
                     VLLEPGR"
     gene            1839..>3312
                     /locus_tag="VF516_22255"
     CDS             1839..>3312
                     /locus_tag="VF516_22255"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8110479.1"
                     /translation="MTQVDSDALRARYAREREKRLRPDGNDQYVELKGHFAKLLEDPY
                     TPRIERPAKHDHVAMTFIGGGFAGLVTTARLKASGLDDVRIVEKGGGFGGTWYWNRYP
                     GAQCDTASMIYMPLLEETGYMPSERYAHAPEIRAHCDRIAARYGLHAGALFHTEATGL
                     EWDPGQSRWIVRTNRGDEFTSQFIGIGTGPLHVAKLPGIPGIEKFAGHSFHTSRWDYA
                     YTGGDPDGAPLSRLADKRVAIIGTGATAVQCIPHLARACRELLVFQRTPSSVDVRANQ
                     PIDSRWFSGIATTGWQDRWLDNFVANMSAAESPSEDLVNDGWTDLAKRIRGRLAAPRP
                     ASATFGDLLADFENADFEKMSEIRARVDAIVEDRDTAEKLKAWYRQLCKRPCFHDEYL
                     DAFNLPSVRLIDTEGKGAERITESGVSVAGVEYPVDCIIYASGFEVGTAPTRRYGFDL
                     IGKDGVRLSEFWSAGMRTLHGIHVRGFPNMFLVQLGHGGNF"
BASE COUNT          560 a         1120 c         1109 g          523 t
ORIGIN      
        1 ccgacgaagc cgcccccgcg tcccagcggc tcgacgtcgc catcgaggta cacggtgtca
       61 ttcggcagcg cgcgccgccc gcggctggcg taactggcga gaacgccgat gccgccatcg
      121 acgtcgaact tcgagtcggc cagcgttgcc cctgtccgcc cggacagggc gtagagatcg
      181 tccctgtgct cacgcaggta ggagccgagc gccttcagca gggccgcgcg ctggtggaac
      241 gtcatctcgc gaagcgcggg gccgccgacc gtacggccgt actcgagcgc ggcggccatg
      301 tcgatgccgt ctgaggagat cctggcgacc tcctcccccg tcaccgcgtc atgcagcggc
      361 gtgccctcac ctgccgggga tcgccattgt ccgccggtgt agctccgcag cacggtcatg
      421 agcccagcct cctcgatggg atcgcttccg gcgaacgtat caaccgggat aacgcgccgg
      481 tcatcgtccg ggttcgagca gcaccttgac cgcgccggtg tgccgcgcgt tcttgagggt
      541 cagtaccgcc tcctgccagc gctccagccg gaaccggtga gtgatcaccg gggtcaggtc
      601 aaagcccgct gcgatgaagt cgaaatagtg ctccatcgag tgcttcgcca cgccgctaac
      661 ggtctcgatg ccaaacccgt tcgagccgat cacgtgcagt tccttgaagt agagcggcgt
      721 ccactcgaac ctctgcggcg gttcgacccc ggacaccacc agcgtgccgc ccgtgtcggc
      781 aaggcgcagc gaggtctcga cggtctcggc cgagccgatc gtgtcataga tcaccgcagg
      841 cccgtcttgc agccaggggc gcttgctcca cggttcgagc ggcgtcgtgt gaacgcggcg
      901 tgcgacctcc gcgatgagtt cctcgggagc ggcaggcaac accgcatccg cgccgagcct
      961 ggtggccagc gcagcccggc tgcccgcccg ggtcgccgcc cagacctcag tgcccggata
     1021 caggtgccgc agcagcgcga tcgcggcgaa agcgagcgtg ccagagccgt acacgagcac
     1081 cggcgcacgg ccggacggcg gcgcgagcag gatcgtgcgc agcgagacgc tgaccgggtc
     1141 ggccagcacg gccgcgtcgt cggagatgtt gtcgggaatc gcgaagagct gcgaggtgtg
     1201 ggcggcgaat cgctccgcat gcgcgcctgc ggcggcggcg cagttcccga tgtggatgga
     1261 gacgggcagg ttgcccgcac ggaagttccg gcaccacgga taacgcccgt tccggcatgc
     1321 cgggcagggc gggtcgatgc cgcgcggccc gcacgacagc cagggattca gcaccacgcg
     1381 ctgaccggtc tcagaaatgc ggccgaccgc ctcgtgtccg agtacgtgcg ggaaggacac
     1441 cagcgcggtc agcgggttgt ccctgcggcc gttgagcacg atctgcttga cgtccgaccc
     1501 gcacagaccg gaccaggtgg tgttcacacg cacccagtcc tgcgctggca gcggcggctc
     1561 gtcgatgtcc tcgaccttga ccggcgcgag ccgtgagaca aaggcgcgac gatcgatccg
     1621 gcccccgatc gtcgccgccg cttcgcggga aaggttgtgc cggaaaacga gcgccttcac
     1681 atctggcccc ttaccggtgc atgccagccc ttgagcggag cccggcccat cgcggattcc
     1741 cttgacgggt cgttcggcgc tgtgagtcac ttgacataat gccaggtaca accctttcat
     1801 gctgaacgcc ggattaatgc acggatgcgc taggtgatat gacccaggtt gacagcgacg
     1861 ctctgcgtgc cagatacgcg cgagagcggg agaagcggct caggccagat ggcaacgacc
     1921 agtacgtcga gcttaagggc cacttcgcca agctcctcga ggacccgtat acgccgcgga
     1981 tcgaacggcc cgccaaacat gatcacgtgg cgatgacgtt catcggcggc ggcttcgcgg
     2041 gcctcgtgac cacggccagg ctcaaggcct ccggcctgga tgatgtccgc atcgttgaga
     2101 aaggcggcgg attcggcggc acctggtact ggaaccgcta cccgggcgcg cagtgcgaca
     2161 ccgcgtcgat gatttacatg ccgctgctcg aggagaccgg ctacatgccg tcagagcggt
     2221 acgcgcatgc tcctgagatt cgggcccact gcgaccggat cgccgcccgc tacggactgc
     2281 atgctggcgc cttgttccat accgaggcga ccggcctgga atgggatccc ggtcaatctc
     2341 gctggatcgt acgcacgaac cgtggcgatg agttcacgag ccagttcatc gggatcggca
     2401 ctggcccgtt gcacgtggcc aagctgccgg ggatcccggg catcgagaag ttcgcggggc
     2461 actcgttcca caccagcagg tgggactacg cctacacggg cggcgacccg gacggcgcac
     2521 cgttgagccg tctggcagat aagcgcgtgg cgatcatcgg cacgggggcg accgctgtgc
     2581 agtgcatccc gcacctggcg cgagcctgcc gggaactgct cgtcttccag cgaacgccct
     2641 cgtcggtcga cgtccgagcc aaccagccga tcgactcgcg atggttctcg gggattgcga
     2701 cgacgggctg gcaggaccgc tggctggaca acttcgtcgc caacatgtcg gccgccgagt
     2761 cgcccagcga ggacctggtc aacgatggct ggaccgacct ggccaagcgc atccgcggca
     2821 ggcttgccgc cccgcgcccg gcctcggcca ccttcggtga tctgctggcg gactttgaga
     2881 acgctgactt cgagaagatg agcgagatcc gtgcccgggt cgacgcgatc gtcgaggacc
     2941 gggacacagc cgagaagctg aaggcgtggt accgccagct ctgcaagcgg ccgtgcttcc
     3001 acgacgagta cctcgacgcc ttcaacctgc cgtcggttcg tctgatcgac accgagggca
     3061 agggcgccga gcggatcaca gaaagcggcg tcagcgtggc cggtgtcgag tacccggtcg
     3121 actgcatcat ctacgcctcg ggattcgagg ttggcacggc gcccacgcgg cgctacggat
     3181 tcgatctcat cggcaaggac ggcgttcgcc tgtcggagtt ctggtccgca ggcatgcgca
     3241 ccttgcacgg catccacgtg cgcggcttcc ccaacatgtt cctcgtgcag cttggccatg
     3301 gcggcaactt ca
//
LOCUS       DASVOR010001168         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_508419,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001168 DASVOR010000000
VERSION     DASVOR010001168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508419"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            841..>2124
                     /locus_tag="VF516_22260"
     CDS             841..>2124
                     /locus_tag="VF516_22260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8110480.1"
                     /translation="MEGDPQMLERLKTRFSDGKTGRLFLTILGAKLLGAGIVLAGIKG
                     IDWYFFTRAYADDAPKIPDHVNALNTVWTLIAAFLVFFMQAGFMCLEAGFARTRETVN
                     VLLEGIVDTMMCGVLFWAFGFAFMFGSGNKFIGMEYFFLHGTPETYGTTGVPMLAYWL
                     FQFAFADTCSTITSGAMVGRTGFKGDILYSIGVSGFIYPIIGHWAWGPDGWLATLTPV
                     AFHDFAGSTVVHTIGGFIALAGAIALGPRLGRKFKRDGGGMPPGHDMTIAAVGGVILW
                     FGWYGFNPGSTLSAMDMAGCARVAANTTLAACSGGLVAMAVVYPRYKVWDCGMTVNGF
                     LAGLVAITCPCYWVSPTGALLLGAVAGVVVVLGCDLLEYLRIDDPVGAVPVHGFCGIW
                     GTLSLGLFATGEFGAPTPTGADTSAGALVTGLFYGG"
BASE COUNT          338 a          676 c          738 g          372 t
ORIGIN      
        1 cctcgggcct ggcgcgcccg cgccgccgag atcgcggcga tcgatgccgg catcctcgcc
       61 cgccggccgg tcgtcgcgcg gatccactga agagcagcag gtgtcgaggg gaggggaaga
      121 agagaaggag gaagcgcgga agggtgaaga gcggaagggg gcgaagagcg gacggggcga
      181 ttcgggacgc aagcgtggtg acgtggacgg tttgacctcg acgttgaccc cgaacgttga
      241 ggttggacgg cgacgtacga cgtcgactcc accgtcgacc tcgacctcga cctcgacgta
      301 cctcgacgtc gacctccgat catcttcgtt cgagcggctg cgacgggcgg cgggtcgcaa
      361 tggatcggcg tttcctcccg gagtcaacgt gcaaggtcaa cggtggggtc aaactctacg
      421 ttgccgtcga agtcaacggt ggggtccagg tcaaggtcca cgtcaaaggc gtcatgacgg
      481 cgacgcgagc agcgtcgtgg cccgtgccag cgcgcggtcg gaccggccgc gatccaggaa
      541 cgatccggtt ccccaatttc cgccatgttt cgttcgtgcg aacgccggtg gcgcgcgacc
      601 ggcgaccggc gctcgcaccc tgtgtgcagg cgggagcgcg cacgccgcga gggggtccag
      661 atttccgccc tgtttcatcg aagcaaactc aaaatagaga atctgtattt gacgatatcc
      721 gcatttgatt gaccacgagc aggcccggcg acaagttacg cactgctcat cggccggaaa
      781 caatgtgagt gcgccgttgc gataaggatc aggccaagat ctcgcaaaaa ctgcatcgac
      841 atggaaggag acccgcagat gctggagcgg ctcaagacgc gattctcgga cggcaagacc
      901 ggtcgactgt tcctgaccat cctcggcgcg aagctgctcg gtgccggcat cgtgctcgcc
      961 gggatcaagg gcatcgactg gtacttcttc accagggcct acgccgatga cgcgcccaag
     1021 atccccgatc acgtcaacgc gctgaacacg gtgtggacgc tgatcgcggc gttcctggtg
     1081 ttcttcatgc aagccggctt catgtgcctg gaggccgggt tcgcgcgcac ccgcgagacc
     1141 gtcaacgtcc tcttggaagg catcgtcgac acgatgatgt gcggcgtgct gttctgggcg
     1201 ttcggcttcg cgttcatgtt cgggtccggc aacaagttca tcggcatgga gtacttcttc
     1261 ctgcacggca cgcccgagac ctacggcacg accggcgtgc cgatgctcgc ctactggctg
     1321 ttccagttcg cgttcgccga cacctgcagc acgatcacct cgggcgcgat ggtcggccgc
     1381 accgggttca agggcgacat cctctacagc atcggcgtca gcggcttcat ctatccgatc
     1441 atcggccact gggcgtgggg gccggacggc tggctcgcga cgctcacccc ggtggcgttc
     1501 cacgacttcg ccggctcgac ggtggtgcac acgatcggtg ggttcatcgc gctcgccggc
     1561 gcgatcgcgc tcggaccgcg gctcggccgc aagttcaagc gcgacggcgg tggcatgccg
     1621 ccgggtcacg acatgacgat cgccgcggtc ggcggcgtga tcctgtggtt cggctggtac
     1681 ggcttcaacc ccggcagcac gctgtcggcg atggacatgg cgggctgtgc gcgggtcgcc
     1741 gccaacacga cgctggcggc gtgcagcggt ggcctggtcg cgatggccgt ggtctacccg
     1801 cgctacaagg tctgggactg cggcatgacg gtcaacggct tcctcgccgg gctggtcgcg
     1861 atcacgtgcc cgtgctactg ggtcagcccg accggcgcgt tgctgctcgg tgcggtcgcc
     1921 ggcgtggtcg tggtgctcgg ctgcgatctg ctcgagtacc tgcggatcga cgatccggtc
     1981 ggtgcggtgc cggtgcacgg cttctgcggg atctggggca cgctcagcct cgggctgttc
     2041 gccaccggcg agttcggcgc tccgacgccc accggcgccg acacctcggc gggcgcgctg
     2101 gtgaccgggc tgttctacgg cggc
//
LOCUS       DASVOR010001169         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_391269,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001169 DASVOR010000000
VERSION     DASVOR010001169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391269"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1588)
                     /gene="sufB"
                     /locus_tag="VF516_22265"
     CDS             complement(176..1588)
                     /gene="sufB"
                     /locus_tag="VF516_22265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003920235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEX8110481.1"
                     /translation="MTLTQSEQIDQLSRYKFGWADADTAGAGAKRGLSEEVVRDISGK
                     KSEPDWMLKLRLKGLKLFERKPLPTWGADLADIDFENIKYFVRSTEKQAASWDDLPDD
                     IKSTYDRLGIPEAEKQRLVAGVAAQYESEVVYHQIQKELEDQGVIFLDTDTGLKEHPE
                     LFREYFGSVIPPGDNKFASLNTAVWSGGSFIYVPKGVHVDIPLQAYFRINTENMGQFE
                     RTLIIVDEDAYVHYVEGCTAPIYSSDSLHSAVVEIIVKKGGRCRYTTIQNWSNNVYNL
                     VTKRALAHEGATMEWVDGNIGSKVTMKYPAVWMVGEHAKGEVLSIAFSGEGQHQDAGA
                     KMVHAAPNTSSTIISKSVARGGGRTSYRGLVQVNEGAHHARSTVKCDALLVDGISRSD
                     TYPYVDVREDDVSMGHEATVSKVSEEQLFYLMSRGLTEDEAMAMIVRGFVEPIARELP
                     MEYALELNRLIELQMEGAVG"
     gene            complement(1585..>2048)
                     /locus_tag="VF516_22270"
     CDS             complement(1585..>2048)
                     /locus_tag="VF516_22270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110482.1"
                     /translation="RPPREFLLTAHGRASFPHAYDDLAAAALRFLAETGGEQAVAEFA
                     ALRVATVEQRHGADVRAADEKVEALAGALTSEGYAASAHKVGSGDQLCQHHCPVAHVA
                     AEFPQLCEAETKAFERLLGTHVQRLATIARGDAVCTTHIPSTSVASGRTRA"
BASE COUNT          277 a          699 c          716 g          356 t
ORIGIN      
        1 agcgccgccc gctcggccgc gccctcgtcg gcggtacccc cgactcctcc cgcggcggcc
       61 gcgagggtcg ggtcaccgtc gtcacggcgg acgccgtcga gcgcctcggc gaccgtcgcg
      121 ggcacgccgg cctcctggcc accggtgacc ccggcggcct gctccggtgc gctcatcagc
      181 cgacggcccc ttccatctgc agctcgatga ggcggttgag ctccagcgcg tattccatcg
      241 ggagctcgcg ggcgatcggc tcgacgaagc cgcgcacgat catcgccatc gcctcgtcct
      301 cggtcaggcc gcggctcatc aggtagaaca gctgctcctc actgaccttc gagaccgtcg
      361 cctcgtgacc catcgacacg tcgtcctcgc ggacgtccac gtaggggtag gtgtcggaac
      421 ggctgatccc gtcgaccagc agcgcgtcgc acttcaccgt cgagcgggcg tggtgcgcgc
      481 cctcgttgac ctgcaccagg ccccgatagg acgtgcggcc accgccgcgg gccacagact
      541 tggagatgat cgtcgaggac gtgttcggcg ccgcgtgcac catcttggcg ccggcgtcct
      601 ggtgctggcc ctcgccggag aacgcgatcg agaggacctc gcccttggcg tgctcgccga
      661 ccatccagac ggccgggtat ttcatcgtca ccttggagcc gatgttgccg tcgacccact
      721 ccatggtcgc gccctcgtgc gccagcgcgc gcttggtgac caggttgtag acgttgttcg
      781 accagttctg gatcgtcgtg tagcggcagc ggccgccctt cttgacgatg atctcgacga
      841 ccgccgagtg gagcgagtcg gacgagtaga tcggcgcggt gcagccctcg acgtagtgca
      901 cgtacgcgtc ctcgtcgacg atgatcagag tccgctcgaa ctggcccatg ttctcggtgt
      961 tgatccggaa gtaggcctgc agcgggatgt cgacgtgcac gcccttcggc acgtagatga
     1021 acgagccgcc ggaccagacc gcggtgttga gcgaggcgaa cttgttgtcg ccgggcggaa
     1081 tgaccgagcc gaagtattcc cggaacagct ccgggtgctc cttcaggccg gtgtcggtgt
     1141 ccaggaagat gacgccctgg tcctccagct ccttctggat ctggtggtag acgacctccg
     1201 actcgtactg cgcggcgacg ccggcgacca gtcgctgctt ctccgcctcg gggatgccga
     1261 gccggtcgta ggtggacttg atgtcgtcgg gcaggtcgtc ccagctggcg gcctgcttct
     1321 cggtggagcg gacgaagtat ttgatgttct cgaagtcgat gtcggcgagg tcggcgcccc
     1381 aggtgggcag cggcttgcgc tcgaagagct tgaggccctt cagtcgcagc ttgagcatcc
     1441 agtccggctc gctcttcttg cccgagatgt cgcggacgac ctcctcggac aggccgcgct
     1501 tggcgccggc gccggcggtg tcggcgtccg cccatccgaa cttgtagcga gacagctggt
     1561 cgatctgctc gctctgggtc aaggtcatgc tcgcgtcctc ccggaagcga cagaggtgct
     1621 ggggatgtgg gtggtgcaga ccgcgtctcc gcgggcgatg gtggccagcc gctggacgtg
     1681 ggtgcccaac agccgctcga acgccttcgt ctctgcctcg cacagctgcg ggaactcggc
     1741 cgcgacgtgc gcgaccgggc agtggtgctg gcagagctgg tcgccggatc cgaccttgtg
     1801 ggccgaggca gcgtatccct cgctggtcag cgcaccggcg agcgcctcga ccttctcgtc
     1861 ggcggcgcgg acgtcggcgc cgtggcgctg ctcgacggtg gcgacgcgga gggcggcgaa
     1921 ctcggccacg gcctgctcgc cgccggtctc ggccaggaac cgcagcgcgg ccgcggccag
     1981 gtcgtcgtac gcgtgcggga agctggcccg gccgtgcgca gtgagcagga actcccgcgg
     2041 cggccggc
//
LOCUS       DASVOR010001170         2002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_508420,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001170 DASVOR010000000
VERSION     DASVOR010001170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508420"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /locus_tag="VF516_22275"
     CDS             <1..1679
                     /locus_tag="VF516_22275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8110483.1"
                     /translation="READMISGRPKEAAPPVAFDQRMLHVEVRKRHAPGRSARFQLEA
                     KLDVSPGVTVIIGRSGAGKTTLLHCIAGLAIPEEGRIAIGDHVLFDSRKKIRLAPAHR
                     GVTFVFQDLALFPHLSVEDNVMYGLRRLDAAERKRRMGEILASFQIEHLAGRFPREIS
                     GGEQQRVALARSLVTEPSVLLLDEPLSSLDPRTKVLIIEDLRRWNQTRRIPILYVTHD
                     HDELLALGDRAIVLEQGKIVTDAPPLDVISNPLRLVTAQKQRFENVFDATVVELREQD
                     GTMVCELTGTSIRVEAPLAEAPPGQVAVGSEVCVGIRADEILVASSQPAILGDCNVIR
                     GKLTRVERNGAMVEARVSCGAEFRVHLGPRSIEPHGLDTSVDIWMMIKTRACHVIRSS
                     LFEALQRLFVFVCQGNTIRSPIAQAICNAEIAARLGVPPESLERFGIAAVSAGLAAQP
                     GVSLAAEAEQALEAIGVPALEHRSRNLTHAMAQRAEVIFCMTEKQRTEVTARFPEASA
                     KTYCLRPDTDVSFPHDGKSADAFSDMAREIQGLIGQKLDGLGVSRPTSPA"
     gene            complement(1663..>2002)
                     /locus_tag="VF516_22280"
     CDS             complement(1663..>2002)
                     /locus_tag="VF516_22280"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8110484.1"
                     /translation="TGVVMLLYLRLSHDARLRTGQLGTQAELDDAIVEGAAKRIRPKV
                     MTVMTMLIGLLPVLWSSGTGADVMRRVAAPMVGGLVSSFLLELIVYPAIYAVWKRRQL
                     RARAELTRGL"
BASE COUNT          329 a          636 c          698 g          339 t
ORIGIN      
        1 cacgggaggc cgacatgatc tcggggaggc cgaaggaggc tgctccgccg gtagcgttcg
       61 atcaaaggat gcttcacgtc gaggtacgca agcgtcacgc tccgggccga tcggcgcgct
      121 tccagctcga ggcaaagctc gatgtatccc cgggcgtgac ggtgatcatc ggccgctccg
      181 gggcgggcaa gacgacgctc ctgcactgca tcgcgggcct ggccatcccc gaggaaggcc
      241 ggattgccat cggcgatcac gtgctgtttg attcgcggaa gaagatccgg cttgcgccgg
      301 cgcaccgcgg ggtcactttc gtattccagg acctggccct gtttccgcac ctgtcggtcg
      361 aggacaatgt catgtacggg ctccgccggc tggacgcggc cgagcggaag cgaaggatgg
      421 gcgagatcct ggcgtcgttt cagatcgagc acctcgcggg ccggttcccc cgagagatct
      481 cggggggcga gcagcagcgg gtcgcgctgg cgcgctcgct ggtcaccgaa ccctcggtgt
      541 tgctcctgga tgagccgctg tcgtcgctcg acccgcgcac gaaggtcctc atcatcgagg
      601 atctgcggcg gtggaaccag acgcgtcgca ttccgatcct gtatgtcacg cacgatcatg
      661 atgaattgct ggcgctgggc gatcgtgcga tcgtgctgga gcaagggaag atcgtcacgg
      721 acgcgccgcc gctcgatgtg atctcgaacc cgcttcgact ggtcacggcg caaaagcaga
      781 ggttcgagaa cgtcttcgat gccaccgtgg tcgagctccg ggagcaggat ggcaccatgg
      841 tctgtgagct caccgggacg tcgatccgcg tcgaggctcc gctggcggag gctccgccgg
      901 ggcaggtcgc cgtgggctcg gaggtgtgcg tgggcattcg cgccgacgag atcctcgtgg
      961 cgtcgtccca gccggccatc ctcggcgatt gcaacgtgat tcgcgggaag ctcacgcggg
     1021 tcgagcggaa cggcgcgatg gtcgaggccc gggtgagctg cggcgccgaa tttcgcgttc
     1081 atctgggccc gcgatcgatc gagcctcacg ggctcgacac gtcggtggac atctggatga
     1141 tgatcaagac ccgcgcgtgc catgtgatcc gatcgagcct gttcgaggcg ttgcagcggc
     1201 tctttgtctt cgtatgccag ggaaatacga ttcgctcgcc gatcgcccag gcgatctgca
     1261 acgccgagat cgccgcccgg ctcggcgtgc caccggagtc actggaacga tttggcatcg
     1321 cggcggtgag cgccggcctg gcggcccagc cgggggtgtc cctcgccgcc gaggccgagc
     1381 aggcgctgga agcgatcggg gtaccggcgc tcgagcaccg gtctcgtaac ctgacgcatg
     1441 ccatggccca gagggcggag gtcatcttct gtatgaccga gaagcagcgc accgaggtga
     1501 cagcgaggtt tccagaggcc tctgcgaaga cgtactgcct gcgccctgac acggacgtca
     1561 gttttcctca tgatggaaaa tcggcggatg cattctccga tatggcccgg gagattcaag
     1621 ggctgatcgg gcagaagctc gacggcctcg gtgtttctcg tcctacaagc cccgcgtgag
     1681 ctccgcccgc gcccgcagct ggcggcgctt ccacaccgcg tagatcgccg gatagacgat
     1741 cagctcgagg aggaacgagc tgaccaggcc gccgaccatg ggcgcggcga cccgccgcat
     1801 gacgtcggcg ccggtgccgc tgctccacaa gacgggcaga aggccgatca gcatcgtcat
     1861 gacggtcatc accttgggcc ggatccgctt ggcggcgccc tccacgatcg cgtcgtcgag
     1921 ctcggcctgg gtcccgagct ggccggtgcg caggcgcgcg tcgtgcgaca gccgcaggta
     1981 gagcagcatc acgacgccgg tc
//
LOCUS       DASVOR010001171         2782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_245708,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001171 DASVOR010000000
VERSION     DASVOR010001171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2782
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245708"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            196..2193
                     /locus_tag="VF516_22285"
     CDS             196..2193
                     /locus_tag="VF516_22285"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8110485.1"
                     /translation="MTAPHATAAGFRVFAQVSFLRWCAGMRRALLLVLGLANGCAHAP
                     AHQPGDEQLKAIKFEGNHQLGDKTLVTGLALHRTERRGGAPDPYLIAIDADRIRGEYL
                     RKGFLDVDVRSRVERRGDAATVTYSVEEGPRAATRVLITGLPPDVPASAVRGKLPLRD
                     GQPFDYDAYDLAKPLLLGVVQDAGYARAQLDARVIADRADRAAVVDLAYTPGPKCKFG
                     NVEIAGATGELATAVRERLAFQPGQVYSTQAVTQTQRNLYGFGRFSTVQVQPDQGTGE
                     VVNVKVAVAEGARHQVQLGGGLGRDTVGYSVHGRAGYSITGWPFALDTASIELRPEYV
                     YNTDTGNEPRLRALARLERADLFWTYTKANIDAGYNYLTWEAYTSYGPLARLGFETPA
                     FTPRVKLRVGWRLEQNAFRNITSIIDPPAKAVMDHPDLINPAAVALKRTLGLDRSERI
                     GGYEQAVVVDLRDKPIEPRLGAYAELRTTEGTALAGGAFNYFEAIGDVRGFAPLLLDG
                     AVLAARARYGGIWGDIPVTERMFLGGAVSQRGFGERRLAPFVHGTTFNQDGTVAGSAY
                     IPYGGAGLFETSVEARVPITTVRTMPVGFVTFLDGGDVTETPGQLDLGNLNWAVGGGL
                     RVITAVGPVRLDVGYRLNRTGGNNPDPSSHFAYHLTIGEAF"
     gene            2193..>2782
                     /locus_tag="VF516_22290"
     CDS             2193..>2782
                     /locus_tag="VF516_22290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110486.1"
                     /translation="MPAQGDHRRTWRWIRRGALGAIGFAMLAVLGAMIAMRTGWGREL
                     VRAQVEQRLAATFTGGATLGGIEGDPFTKLTLHDVVINGADRRPAITVKHLTVTVGLL
                     PLLSHQARVAAVHADGVDVDLRRDRRGALVTQDLMRPGPPSAWSVDLPEVAIRHGHVR
                     YDTGSEVIDLDGVDLDAWAQLPHAGPVDAGASLRGT"
BASE COUNT          371 a         1028 c         1012 g          371 t
ORIGIN      
        1 gcgatgttcc tcagcggcca catcgtcggt cagatcgccg cggtcgcgct cgccgtgatc
       61 gcgatcccgg ccctggtcac cggcctccgc ggcaaggcgg agcgcgagcg cgaccgggtc
      121 cacccgtcgc gctgagcgcg cggccgctcg ccgcgcccgg cggctcgttc accgcgctcg
      181 cccgacacgc tctgtgtgac cgccccgcac gccaccgctg cggggtttcg tgtatttgcg
      241 caggtatcgt tcttgcggtg gtgcgctggc atgcggcgcg cgctgctcct ggttctcggg
      301 ctcgcgaacg gctgtgccca tgccccggcg caccaacccg gcgacgagca actcaaggcg
      361 atcaagttcg agggcaacca tcagctcggt gacaagacgc tggtcaccgg gctcgcgctg
      421 caccggaccg agcgccgggg cggcgcgcct gatccgtacc tgatcgcgat cgacgccgac
      481 cggatccgcg gcgagtacct gcgcaagggc ttcctcgacg tcgatgtccg atcgcgggtc
      541 gagcgccggg gcgacgcggc gacggtgacc tacagcgtcg aggagggccc gcgcgcggcg
      601 acccgcgtcc tgatcaccgg gctgccgccc gacgtcccgg cctcggcggt gcgcggcaag
      661 ctgccgctgc gcgacggcca gccgttcgac tacgacgcct acgacctcgc caagccgctg
      721 ctgctcggcg tggtccagga cgccggctac gcgcgcgccc agctcgacgc ccgcgtgatc
      781 gccgaccgcg cggaccgcgc cgcggtggtc gacctcgcct acacccccgg gccgaagtgc
      841 aagttcggca acgtcgagat cgccggcgcg accggcgagc tggcgaccgc ggtgcgcgag
      901 cggctcgcgt tccagcccgg ccaggtgtac tcgacccagg cggtcaccca gacccagcgc
      961 aacctgtacg gcttcggccg gttctcgacc gtccaggtcc agcccgatca gggcaccggc
     1021 gaggtggtca acgtcaaggt cgcggtcgcc gagggcgcgc gccaccaggt ccagctcggc
     1081 ggcgggctcg gccgggacac ggtcgggtac tcggtgcacg gccgcgccgg gtactcgatc
     1141 accggctggc cgttcgcgct cgacaccgcg tcgatcgagc tgcgccccga gtacgtctac
     1201 aacaccgaca ccggcaacga gccgcggctg cgcgcgctcg cccggctgga gcgcgccgat
     1261 ctgttctgga cctacaccaa ggccaacatc gacgccggct acaactacct gacctgggag
     1321 gcgtacacga gctacgggcc gctcgcgcgg ctcgggttcg agaccccggc ctttaccccg
     1381 cgcgtgaagc tgcgggtcgg ctggcggctc gagcagaacg cgttccgcaa catcacgtcg
     1441 atcatcgatc cgccggccaa ggcggtgatg gaccaccccg acctgatcaa ccccgccgcg
     1501 gtcgcgctga agcgcacgct cgggctcgat cgcagcgagc ggatcggcgg ctacgagcag
     1561 gcggtggtcg tcgacctgcg cgacaagccg atcgagccgc ggctcggggc gtacgccgag
     1621 ctgcgcacca ccgagggcac ggccctggcg ggcggggcgt tcaactactt cgaggcgatc
     1681 ggcgacgtgc gcggcttcgc gccgctgctg ctcgacggcg ccgtgctcgc ggcgcgcgcc
     1741 cgctacggcg ggatctgggg cgacatcccg gtgaccgagc ggatgttcct gggcggcgcg
     1801 gtcagccagc gcgggttcgg cgagcgccgg ctcgcgccgt tcgtccacgg caccacgttc
     1861 aaccaggacg gcacggtcgc cggctcggcg tacatcccgt acggcggcgc cggcctgttc
     1921 gagaccagcg tggaggcccg ggtgccgatc accacggtcc ggaccatgcc ggtcgggttc
     1981 gtcacgttcc tcgacggcgg cgacgtcacc gagacgccgg gccagctcga cctgggcaac
     2041 ctgaactggg cggtgggcgg cggcctgcgg gtgatcacgg cggtcggacc ggtccggctc
     2101 gacgtcggct accggctcaa ccgcaccggc gggaacaacc cggaccccag ctcgcacttc
     2161 gcgtaccacc tcacgatcgg ggaggcattc tgatgccggc gcagggcgat caccggcgga
     2221 cctggcggtg gatccggcgc ggcgcgctcg gcgcgatcgg gttcgcgatg ctcgccgtgc
     2281 tcggggcgat gatcgcgatg cggaccgggt ggggccgcga gctggtccgt gcccaggtcg
     2341 agcagcggct cgccgcgacg ttcaccggcg gggcgacgct cggcgggatc gagggcgacc
     2401 cgttcaccaa gctcacgctg cacgacgtcg tgatcaacgg cgccgaccgc cggccggcga
     2461 tcaccgtgaa gcacctgacc gtcacggtcg ggctgttgcc gctgctgtcc caccaggcgc
     2521 gggtggccgc ggtgcacgcc gacggcgtcg acgtcgatct ccggcgcgac cggcgcggcg
     2581 cgctggtgac ccaggacctg atgcggccgg ggccgccgtc ggcgtggagc gtcgatctgc
     2641 ccgaggtcgc gatccgccac ggccacgtgc gctacgacac cggcagcgag gtgatcgatc
     2701 tcgacggggt cgatctcgac gcgtgggccc agctgccgca cgccgggccg gtcgacgccg
     2761 gcgcgtcgct gcgcgggacg tg
//
LOCUS       DASVOR010001172         2880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420311,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001172 DASVOR010000000
VERSION     DASVOR010001172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420311"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1994)
                     /locus_tag="VF516_22295"
     CDS             complement(<1..1994)
                     /locus_tag="VF516_22295"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8110487.1"
                     /translation="MRDPKQLDARVSAARQAVEARGETFYPGASRIHLAAFPPKERWG
                     DWVEVDARTPDRRERRYVLVPTTCFNCESACGLLAYVDRDSLEVRKLEGNPEHPGSRG
                     RNCAKGPATLNQVTDPDRILTPLKRAGERGEGRWAAVSWDEALDDIAARIRRAITDGR
                     GNEVMYHVGRPGEDGFTERVLAAWGVDGHNSHTNICSSSGRAGYHYWMGFDRPSPDYA
                     NADVIVLVSAHLESGHYFNPHAQRIVEARARGAKLIVLDVRLSNTASQADHWIAPQPG
                     SEAAIFLAIAQHLIATGRHDRGFIERWWNWQEYMAVALGRPGASFAEFEAELAAMWSR
                     YTFELAAREAGVDAAALRAIADDIAGAGTRLAAHVGRSAAAGNLGGWQVSRTLFLLNA
                     LLGAVACEGGTSPNGWNKFVPRPIHMPPHPHTWNELTWPVEYPLAMNELSFLLPHLVG
                     DGRGALEVYFTRVYNPVWTNPDGFSWIEMLTDPEKVRLHVALTPTWNETAQYADYVLP
                     MGVGSERHDLHSYETHDAQWLGFRQPVLRAARERLGGPVADTRHANPGEVWEENEFWI
                     ELSWRIDPDGALGIRRHFESRERPGERLGIDEYYRYIFEHSVPGLPERAAAEGLAPLD
                     YMRRYGAFEVRRGIGPVHAEAVPAAELDDAATDALGRVFTR"
     gene            complement(1987..>2880)
                     /gene="nrfD"
                     /locus_tag="VF516_22300"
     CDS             complement(1987..>2880)
                     /gene="nrfD"
                     /locus_tag="VF516_22300"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX8110488.1"
                     /translation="HPERANSAAAVRLAYDVAHRAPWDARVSLYTWTKGIAAGVYLVG
                     LLLLALGAIDAGGAVWRWSVPIAAVAAVAVTGAVLSADLDHPERCLLIFTRRHGRSWL
                     VRGAFVLVGYGGVLAVHLVTSLLGAPGAPVPLSVWLAIAGGPLAAMAAVYTAFLFAQC
                     KARDLWQSPLLPPHLIVQAVVAGAAVLLLIDTLAGGAAVGPGPTLARILAIASLAHVA
                     VALAEIALTHGTAHARLAAHEMTRGRYRRWFWAGLALTALGALGALAPWLPVAPPIAA
                     LAGLYAFEHAHVQAGQAVPLA"
BASE COUNT          383 a         1076 c         1065 g          356 t
ORIGIN      
        1 cgggtgaaca cccggccgag cgcgtcggtc gccgcgtcgt cgagctcggc cgccggcacg
       61 gcctcggcgt ggaccggccc gatgccgcgc cggacctcga acgcgccgta gcgccgcatg
      121 tagtcgagcg gcgcgaggcc ctcggccgcg gcgcgttcgg gcagcccggg caccgagtgc
      181 tcgaagatgt agcggtagta ctcgtcgatg ccgagcctct cccccggccg ctcgcgcgac
      241 tcgaagtggc ggcggatgcc gagcgcgccg tcgggatcga tccgccacga cagctcgatc
      301 cagaattcgt tctcctccca gacctcgccg gggttggcgt gccgggtgtc ggcgaccgga
      361 ccgccgagcc gctcgcgcgc cgcgcgcagc accggctggc gaaacccgag ccactgcgcg
      421 tcgtgggtct cgtacgagtg caggtcgtgg cgctcgctgc cgacccccat cggcagcacg
      481 tagtcggcgt actgcgcggt ctcgttccag gtcggggtca gcgcgacgtg gagccggacc
      541 ttctcgggat cggtcagcat ctcgatccac gagaacccgt cggggttggt ccacaccggg
      601 ttgtagaccc gcgtgaagta gacctcgagc gcgccgcggc cgtcgccgac caggtgcggc
      661 aacaggaacg acagctcgtt catcgccagc gggtactcga ccggccaggt cagctcgttc
      721 caggtgtgcg ggtgcggcgg catgtggatc ggccgcggca cgaacttgtt ccacccgttg
      781 ggggacgtcc cgccctcgca cgcgaccgcg ccgagcagcg cgttgagcag gaacagcgtg
      841 cgcgacacct gccagccgcc gaggttgccg gccgcggcgc tgcgccccac gtgggccgcc
      901 agccgcgtgc cggcgccggc gatgtcgtcg gcgatcgcgc gcagcgccgc ggcatcgacc
      961 ccggcctcgc gcgccgcgag ctcgaacgtg tagcgcgacc acatcgccgc cagctcggcc
     1021 tcgaactcgg cgaagctcgc gccggggcgg ccgagcgcga ccgccatgta ctcctgccag
     1081 ttccaccagc gctcgatgaa gccgcggtcg tgccgcccgg tcgcgatcag gtgctgggcg
     1141 atcgcgagga agatcgccgc ctcgctgccg ggctgcggcg cgatccagtg gtcggcctgg
     1201 ctcgcggtgt tcgacagccg gacgtcgagc acgatcagct tggcgccgcg cgcccgggcc
     1261 tcgacgatcc gctgggcgtg cgggttgaag tagtggccgg actccaggtg cgcgctgacc
     1321 agcacgatca cgtcggcgtt ggcgtaatcg gggctcggcc ggtcgaaccc catccagtag
     1381 tggtagccgg cgcggccgct cgacgagcag atgttggtgt gcgagttgtg gccgtcgaca
     1441 ccccacgccg cgagcacgcg ctcggtgaag ccgtcctcgc cggggcggcc gacgtggtac
     1501 atgacctcgt tgccgcggcc gtcggtgatc gcgcggcgga tccgcgcggc gatgtcgtcc
     1561 agcgcctcgt cccacgacac cgcggcccac cggccctcgc cgcgctcgcc ggcgcgcttg
     1621 agcggcgtga ggatgcggtc ggggtcggtg acctggttca gcgtcgcggg gcccttggcg
     1681 cagttgcggc cgcgcgagcc ggggtgctcg gggttgccct cgagcttgcg gacctccagg
     1741 ctgtcgcggt cgacgtacgc caggagcccg cacgccgact cgcagttgaa gcaggtcgtc
     1801 ggcaccagca cgtagcggcg ctcgcggcgg tcgggcgtgc gcgcgtcgac ctcgacccag
     1861 tcgccccacc gctccttcgg tgggaacgcg gcgaggtgga tccggctcgc gccggggtag
     1921 aacgtctcgc cgcgcgcctc gaccgcctgg cgcgccgccg acacccgcgc gtcgagctgc
     1981 ttcggatcac gcaagcggca ccgcctgccc ggcctggacg tgggcgtgct cgaacgcgta
     2041 gaggccggcg agcgcggcga tcggcggcgc gaccggaagc cacggcgcca gcgcgccgag
     2101 cgccccgagg gcagtgagcg ccaggccggc ccagaaccat cgccggtagc ggccgcgcgt
     2161 catctcgtgc gccgcgagcc gcgcatgcgc cgtgccgtgg gtcagcgcga tctcggcgag
     2221 cgcgaccgcc acgtgcgcca gcgacgcgat cgccaggatc cgcgccagcg tggggccggg
     2281 gcccacggcg gcgccacccg cgagcgtgtc gatcaggagc agcaccgcgg cgccggccac
     2341 gaccgcctgc acgatcaggt gcggcggcag cagcgggctc tgccacagat cgcgcgcctt
     2401 gcactgggcg aacaggaacg cggtgtacac cgcggccatc gccgcgagcg gcccgccggc
     2461 gatcgcgagc cacaccgaga gcggcacagg cgcgccgggc gcgccgagca gcgaggtgac
     2521 caggtgcacc gccagcacgc cgccgtagcc gaccagcacg aacgcgccgc gcaccagcca
     2581 gctccggccg tggcggcggg tgaagatcag caggcaccgc tccgggtgat cgagatccgc
     2641 gctcagcacg gcgccggtca ccgcgacggc ggccaccgcg gcgatcggca ccgaccaccg
     2701 ccacaccgcg ccgcccgcgt cgatcgcgcc gagcgcaagc aggagcagcc cgaccaggta
     2761 caccccggcc gcgatgccct tggtccacgt gtacaggctg acccgcgcgt cccatggcgc
     2821 gcggtgcgcc acgtcgtacg cgagccgcac cgccgcggcg ctgttggcgc gctccgggtg
//
LOCUS       DASVOR010001173         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_479512,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001173 DASVOR010000000
VERSION     DASVOR010001173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479512"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VF516_22305"
     CDS             <1..372
                     /locus_tag="VF516_22305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110489.1"
                     /translation="ERKAILVFDDSPFALALTRAALEAAGFTVAIAADLSSFERQRVA
                     FDPDLILVDVQMPEAFGDDVASTLRGWHGVQVPILLVSSLDQGELERRAERAQASGYI
                     CKGAGMSELVRRCRELLEVAR"
     gene            403..717
                     /locus_tag="VF516_22310"
     CDS             403..717
                     /locus_tag="VF516_22310"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8110490.1"
                     /translation="MDDLSAKFLPQFLELARSRVATAIQAAVARDHLAAPTIARNLHT
                     LAGEAGLLGLHDVVPLAREGEHKAKSLGASRTDADAELLVDALRRLERVIESIGATST
                     SK"
     gene            800..1708
                     /locus_tag="VF516_22315"
     CDS             800..1708
                     /locus_tag="VF516_22315"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8110491.1"
                     /translation="MTVDLGQRVEELLRENRLLREELDAKTALAARTVASFQQRTLLM
                     EITRQQNDDLDRLAAELARAKKIEEERAREIEAAARLKSEFLANFSHEIRTPLNAITG
                     YCDLLIRDEGGRLTPHGRRDLSVIKANARTLLALINDILDLSKIEAGRAEVVKEAISL
                     PDLAEECMASVRELLKGKEVALGSEITVGPVFTDPLKLRQVLLNLLSNAAKFTDTGEI
                     VLTAEPQGGTLVISVEDTGSGIPDDQLPFIFDKFRQVDGSARRKIGGTGLGLAIAKEL
                     SAALGGRIELRSEVGRGSRFELVLPA"
BASE COUNT          267 a          591 c          618 g          239 t
ORIGIN      
        1 gagcgcaagg cgatcctggt gttcgacgac agcccgttcg cgctcgcgct gacccgcgcc
       61 gcgctcgagg ccgccgggtt caccgtcgcg atcgccgccg atctgtcgtc gttcgagcgc
      121 cagcgcgtcg cgttcgatcc cgacctgatc ctggtcgacg tccagatgcc ggaggcgttc
      181 ggcgacgacg tcgcgtcgac gctgcgcggc tggcatggcg tccaggtgcc gatcctgctg
      241 gtgtcgagcc tggaccaggg cgagctggag cgccgggccg agcgcgccca ggcctcgggc
      301 tacatctgca agggggccgg catgtcggag ctggtgcggc ggtgccggga gctgctggag
      361 gtcgcgcgct gaccggcgct ggcgagcgaa gcgaaaccga gcatggacga cctcagcgcc
      421 aagtttctgc cccagttcct ggagctcgcg cgatcgcggg tggccacggc gatccaggcc
      481 gcggtcgcgc gcgaccacct cgcggcgccc acgatcgcgc gcaacctcca taccctcgcc
      541 ggtgaggccg gcctgctggg actgcacgac gtggtcccgc tggcgcgcga gggcgagcac
      601 aaggccaagt ctctgggcgc gagccggacc gacgccgacg ccgagctgct ggtggacgcg
      661 ctgcgccggc tggaacgagt gatcgagagt atcggggcga catccacgtc gaagtgagga
      721 gagtcatgac cgagctacgg ttgacgttgt tcaccgcagt agagggtcgt accaccgccg
      781 ggcgagcggg agccccgtca tgaccgtgga tctcggacag cgcgtcgagg agctgctgcg
      841 ggagaaccgg ctgctgcgcg aggagctcga cgccaagacc gcgctcgccg cgcgcacggt
      901 cgcgagcttc cagcagcgca cgctgctcat ggagatcacg cgccagcaga acgacgacct
      961 cgaccggctc gccgccgagc tcgcgcgcgc gaagaagatc gaggaggagc gggcgcgcga
     1021 gatcgaggcc gcggcgcggc tcaagagcga gttcctcgcc aacttctcgc acgagatccg
     1081 gaccccgctc aacgcgatca ccgggtactg cgacctgctg atccgcgacg agggcggccg
     1141 gctcacgccg cacggccgcc gcgacctgtc ggtgatcaag gccaacgcgc gcacgctgct
     1201 cgcgctgatc aacgacatcc tcgatctgtc caagatcgag gccgggcgcg ccgaggtcgt
     1261 caaggaggcg atctcgctgc ccgatctggc cgaggagtgc atggccagcg tgcgcgagct
     1321 gctgaaaggt aaggaggtcg cgctcggctc cgagatcacg gtgggcccgg tgttcaccga
     1381 tccgctcaag ctccgccagg tcctgctcaa cctgctgtcc aacgcggcca agttcaccga
     1441 caccggcgag atcgtgctga ccgcggagcc ccagggcgga acgctggtga tctcggtcga
     1501 ggacaccggc tcggggatcc ccgacgacca gctgccgttc atcttcgaca agttccgcca
     1561 ggtcgacggc tcggcgcgcc gcaagatcgg cggcaccggc ctcggtctgg cgatcgcgaa
     1621 ggagctctcc gccgcgctcg gcgggcggat cgagctgcgc tccgaggtcg ggcgcggctc
     1681 gcgcttcgag ctcgtcctgc cggcctagag ctagg
//
LOCUS       DASVOR010001174         4355 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_333182,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001174 DASVOR010000000
VERSION     DASVOR010001174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333182"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2984)
                     /locus_tag="VF516_22320"
     CDS             complement(<1..2984)
                     /locus_tag="VF516_22320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8110492.1"
                     /translation="MIQRIIRFSADHRYLVIAATIVALGFSWWSVRTIPLDALPDLSD
                     TQVIVYARWDRSPDLVEDQVTYPITTALLGAPRVKAIRGFSDFGFSYVYVIFEDGTDL
                     YWARSRVLEYLSKITSQLPAGVKAELGPDASSVGWVFQYALVDRTGTHSSDELRSYQD
                     WFLRYAVQSVPGVAEVATVGGQVRQYQVTVNPNQLVAYKLPLDTVIDAVRKGNSDVGG
                     RLVELSGREYMVRGRGYVKDIHDLENIVLRSDGGTPVLIKDVAKVALGPEMRRGIADY
                     DGKGDVVGGIVVMRAGENALGVIGRVKAKLDEVKPSLPAGVEVVTTYDRSELIGRAIA
                     TVEHKLIEEIVIVSLIILVFLWHFPSSIVPILTIPVSVALAFIPMRLMGIDANLMSLA
                     GIAISIGVLVDGAIVEVENAYNKIHRWRAAGGRGDFHRVRLEALLEVGPGVFFSLLVI
                     AVAFMPVFTLVDQEGRLFRPLAFSKNLAMAIAAILAITLDPAIRMLFARIEPFRFRPR
                     WLAWLATRIAVGTYHAEDRHPVSRLLQRLYEGPCRFVLRHPRATVAAAVAIVATAVPV
                     YLHLGSEFMPPLREGTLLYMPSAVEPGMSVAEAQKALQLQDKLLMTFPEVAGVFGKAG
                     RASTSTDPAPFTMMETTVLLKPETEWREKPRWYSSWAPGWLKPALRPLWPDRISDAEL
                     EDQMNRALRLPGISNAWTMPIKGRLDMLSTGIRTPVGIKIVGSDLAVIERIARQTEAA
                     VARVPGTRSAYAERVAGGYFLDFVLKRDRLARYGLSIDDANTMVITAVGGDNQSVTVE
                     GRERYGINVRYARDYREDLQALRRVLLPLPGAQGQIPMEEIADVVLAHGPAMIRDENG
                     LLAGYVYVDFDTSQIDVGHYVEQAKRAVAAGVTLPTGTSMMWSGQYENMLRVKERLKL
                     ILPLTLVVIFALLYLNTRSAFKATVVMLAVPFSAVGAVWLLALLGYNISIAVWVGMIA
                     LMGLDAETGVFMLLFLDLSHDEA"
     gene            complement(2981..>4355)
                     /locus_tag="VF516_22325"
     CDS             complement(2981..>4355)
                     /locus_tag="VF516_22325"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8110493.1"
                     /translation="TAVLVVAPWRAAGGGEPSAAATLYQCPMHPSIVQDHPGECPICG
                     MKLVAAPHGAPGPAGEPVRKVAFYRSPMDPRQTSPAPRKDAMGMDYVPVYEDEVAGPA
                     VDGLAAVEIDPVRQQLIGLKTTEVVWGDVGGAWRTVGRVAIDETRVRHVNLKVAGFVE
                     RIYIDFVGKKVRRGDPLFSIYSPELLAAQEEYLLALRTRTALARGTGGAPVGAPAADD
                     GEVLVAAARRKLALWDVPGEEIERIARTGEPSKTLTLYSPVAGVVTRKDVVEGMKLDA
                     GAMPYEIVDLSSLWVLADVYESELRFVREGMPATLKLHAFPNREFTGQVVFIDPLLNP
                     QTRTVKLRLTFANPDGELRPEMFGEVVLRGAPHQGLRIPPDAIIDSGIDKVVFVAVGE
                     GKFAPRKVRLGEADGAHVEVITGLAQGERVVSRANFLIDSESRLRASLAELSGAAPEP
                     ERARVLP"
BASE COUNT          665 a         1565 c         1520 g          605 t
ORIGIN      
        1 gcctcgtcgt gcgacaggtc gaggaacagc agcatgaaca cgccggtctc ggcgtcgagc
       61 cccatcagcg cgatcatccc gacccacacg gcgatcgaga tgttgtagcc gagcagcgcg
      121 agcagccaca ccgcgccgac cgccgagaac ggcaccgcga gcatcacgac cgtcgccttg
      181 aacgccgacc gcgtgttcag gtagagcagc gcgaagatca cgaccagggt gagcggcagg
      241 atcagcttga gccgctcctt gacccgcagc atgttctcgt actggccgct ccacatcatc
      301 gaggtgccgg tcggcagggt gacgccggcg gcgaccgcgc gcttggcctg ctcgacgtag
      361 tggccgacgt cgatctgcga ggtgtcgaag tcgacgtaga cgtagccggc gagcaggccg
      421 ttctcgtcgc ggatcatcgc cgggccgtgg gccagcacga cgtcggcgat ctcctccatc
      481 gggatctggc cctgggcgcc gggcagcggc aagagcacgc ggcgcagcgc ctggaggtcc
      541 tcgcggtagt cgcgcgcgta gcgcacgttg atgccgtagc gctcgcggcc ctcgacggtc
      601 acgctctggt tgtcgccgcc gaccgcggtg atcaccatgg tgttggcgtc gtcgatcgac
      661 agcccgtagc gcgcgagccg atcgcgcttc agcacgaagt cgaggaagta gccgccggcg
      721 acgcgctcgg cgtacgcgct gcgcgtgccg gggaccctgg ccaccgcggc ctcggtctgc
      781 cgggcgatcc gctcgatcac cgcgagatcg gagccgacga tcttgatgcc gaccggggtg
      841 cggatgccgg tcgacagcat gtcgagccgg cccttgatcg gcatggtcca ggcgttcgag
      901 atcccgggca gccgcagcgc gcggttcatc tggtcctcga gctcggcgtc gctgatccga
      961 tcgggccaca gcggccgcag cgccggcttg agccagccgg gcgcccacga cgaataccac
     1021 cgcggcttct cgcgccactc ggtctcgggc ttgagcagca cggtggtctc catcatcgtg
     1081 aacggcgccg gatcggtcga cgtgctggcg cggccggcct tgccgaacac gccggcgacc
     1141 tcggggaacg tcatcaggag cttgtcctgg agctgcagcg ccttctgcgc ctcggcgacc
     1201 gacatgccgg gctcgacggc cgacggcatg tacaggagcg tgccctcgcg cagcggcggc
     1261 atgaactccg agccgaggtg gagatacacc ggcaccgcgg tggcgacgat cgcgacggcg
     1321 gccgccacgg tcgcccgcgg gtggcgcagc acgaaccggc acggcccctc gtacagccgc
     1381 tggagcagcc ggctgaccgg gtggcggtcc tcggcgtggt aggtcccgac cgcgatccgg
     1441 gtcgcgagcc acgccagcca gcgcggccgg aaccggaacg gctcgatccg cgcgaacagc
     1501 atccggatcg ccgggtcgag cgtgatcgcc aggatcgccg cgatcgccat cgccaggttc
     1561 ttggagaacg ccagcggccg gaacagccgg ccctcctggt cgaccagcgt gaacaccggc
     1621 atgaacgcca ccgcgatgac cagcagcgag aagaacacgc ccgggccgac ctcgagcagc
     1681 gcctcgagcc gcacgcggtg gaagtcgccg cgcccgcccg cggcgcgcca gcggtggatc
     1741 ttgttgtagg cgttctcgac ctcgacgatc gcgccgtcga ccagcacgcc gatcgagatc
     1801 gcgatcccgg ccagcgacat gaggttggcg tcgatcccca tcagcctcat cgggatgaac
     1861 gccagcgcga ccgacaccgg gatcgtgagg atcgggacga tcgacgacgg gaagtgccac
     1921 aggaacacca ggatgatcag cgagacgatg acgatctcct cgatcagctt gtgctcgacc
     1981 gtggcgatcg cgcgcccgat cagctccgag cggtcgtagg tggtgacgac ctcgacgccg
     2041 gccggcagcg acggcttgac ctcgtcgagc ttggccttga cccggccgat cacgccgagc
     2101 gcgttctcgc ccgcgcgcat cacgacgatg ccgccgacca cgtcgccctt gccgtcgtag
     2161 tcggcgatcc cgcgccgcat ctcggggccg agcgcgacct tggcgacgtc cttgatcagg
     2221 accggcgtgc caccgtcgct gcgcagcacg atgttctcga ggtcgtggat gtccttgacg
     2281 tagccgcggc cgcgcaccat gtactcgcgg cccgacagct cgaccagccg gccgccgacg
     2341 tcgctgttgc ccttgcggac cgcgtcgatc acggtgtcga gcggcagctt gtaggcgacc
     2401 agctggttgg ggttgacggt gacctggtac tggcggacct ggccgccgac ggtggcgacc
     2461 tcggcgacgc ccggcacgct ctgcaccgcg tagcgcagga accagtcctg gtacgagcgc
     2521 agctcgtcgg acgagtgcgt gccggtgcgg tcgaccagcg cgtactggaa cacccagccg
     2581 acgctcgacg cgtcggggcc gagctcggcc ttgacccccg ccggcagctg cgaggtgatc
     2641 ttcgacaggt actcgagcac ccggctgcgc gcccagtaca ggtccgtgcc gtcctcgaag
     2701 atcacgtaga cgtacgagaa cccgaagtcg gagaacccgc ggatcgcctt gacccgcggc
     2761 gcgccgagca gcgccgtcgt gatcggatag gtcacctggt cctcgacgag gtccgggctg
     2821 cggtcccagc gcgcgtagac gatgacctgg gtgtcggaca gatcgggcag cgcgtcgagt
     2881 gggatcgtcc gcaccgacca ccacgagaag cccagggcga cgatggtcgc cgcgatcacg
     2941 aggtagcggt ggtcggccga gaaccggatg atccgctgga tcatggcagc acgcgggcgc
     3001 gctcgggctc gggcgcggcg ccggacagct cggcgagcga cgcgcgcagc cgcgactccg
     3061 agtcgatcag gaagttggcc cggctcacca cgcgctcgcc ctgcgccagg ccggtgatga
     3121 cctcgacgtg cgcgccgtcg gcctcgccga gccggacctt gcggggcgcg aacttgccct
     3181 cgcccaccgc gacgaacacc accttgtcga tgccggagtc gatgatcgcg tcgggcggga
     3241 tccgcaggcc ctggtgcggc gcgccgcgca gcacgacctc gccgaacatc tccggccgga
     3301 gctcgccgtc ggggttggcg aacgtcagcc ggagcttgac cgtccgggtc tgcgggttga
     3361 gcagcggatc gatgaacacg acctggccgg tgaactcgcg gttgggaaac gcgtggagct
     3421 tgagcgtcgc cggcatgccc tcgcgcacga accgcagctc gctctcgtag acgtcggcga
     3481 gcacccacag cgacgacagg tcgacgatct cgtacggcat cgcgccggcg tcgagcttca
     3541 tgccctcgac cacgtccttg cgggtgacca cgccggcgac cggcgagtac agcgtgagcg
     3601 tcttgctcgg ctcgccggtg cgcgcgatcc gctcgatctc ctcgcccggc acgtcccaca
     3661 gcgcgagctt gcggcgcgcc gcggcgacca gcacctcgcc gtcgtcggcg gccggcgccc
     3721 ccaccggcgc cccgccggtg ccgcgcgcca gcgccgtgcg ggtcctcagc gcgagcaggt
     3781 actcctcctg cgccgcgagc agctcggggc tgtagatcga gaacagcgga tcgccgcgcc
     3841 gcaccttctt gccgacgaag tcgatgtaga tccgctcgac gaagcccgcg accttgaggt
     3901 tgacgtggcg gacccgggtc tcgtcgatcg cgacccgccc gaccgtccgc cacgccccgc
     3961 cgacgtcgcc ccacaccacc tcggtggtct tgagcccgat cagctgctgg cgcaccggat
     4021 cgatctcgac cgcggcgagc ccgtcgaccg ccggaccggc gacctcgtcc tcgtagaccg
     4081 gcacgtagtc catccccatc gcgtccttgc gcggcgccgg cgaggtctgc cggggatcca
     4141 tcggcgagcg gtagaacgcg accttgcgca ccggctcgcc cgcgggcccg ggcgccccgt
     4201 gcggcgccgc gaccagcttc atgccgcaga tcggacattc gccggggtgg tcctgcacga
     4261 tcgacgggtg catcggacac tggtagagcg tcgcggcggc ggacggctcg ccaccgcccg
     4321 cggcgcgcca cggcgcgacg acgagcaccg cggtg
//
LOCUS       DASVOR010001175         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_274193,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001175 DASVOR010000000
VERSION     DASVOR010001175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274193"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VF516_22330"
     CDS             <1..877
                     /locus_tag="VF516_22330"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HEX8110494.1"
                     /translation="PAQLADLEFKDNAGLAAMVVKNRHYLPAGGEPREVSAPIYTRKV
                     KIEDARSLLVLPLLAADEPVGTLTLVARAEKRFGKDVREMLSVIANQVAIALENGFLY
                     RKMETMATTDGLTGLTNHRTFQQRFADMLERAARHNHKVAVLLCDVDHFKKVNDNYGH
                     PVGDEVLRRVARVLQEVPRKIDIPARYGGEEFAVLLDNVDVAQAKQVAERIRIEISRL
                     VIESEKGPLSVTESIGVAAFPDDGRDRATLIERADLALYHAKHTGRNRVVTWAEAQAA
                     KGGHAQSAQSAKKAS"
     gene            1052..1339
                     /locus_tag="VF516_22335"
     CDS             1052..1339
                     /locus_tag="VF516_22335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110495.1"
                     /translation="MHLRVIALFVAVVVASGAALAVMVSASPAVAASPDVAGLVDDGA
                     LDPEPAIAAAPVALPVLVRRELRPVVASPGELPGRLHSASVFRPPRRFALP"
     gene            1375..2313
                     /locus_tag="VF516_22340"
     CDS             1375..2313
                     /locus_tag="VF516_22340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX8110496.1"
                     /translation="MSELVVPAWAWALLAGVMLLLIAIDLFAHRGDRSDSRRLALIWT
                     VVWIAAAVVFGAFVAVWFGAAAAEQYFAAYLLEKSLSVDNLFLFVVVFGALDIPVSEQ
                     RRVLTWGILGALVTRGVFIALGAAILHRWHEVTYVFGAILVITALKLLRGGEGRPGRL
                     LIWLERHLPWTRERSGHRFLVRRDGRWLATPLLVALLAIELTDVMFAIDSIPAAFAVS
                     DEPFVIYSSNVFAVLGLRALYLVLVGALADLRYLKYGLSAVLAFAGSKMLASPWIQIS
                     PIVSVSVIAVVIGIAAGASVWAVRRDHRRGRMGASP"
BASE COUNT          314 a          856 c          960 g          423 t
ORIGIN      
        1 tccggcgcag ctggccgacc tcgagttcaa ggacaacgcg gggctggcgg cgatggtggt
       61 caagaaccgg cactacctgc cggcgggcgg cgagccgcgc gaggtgtcgg cgccgatcta
      121 cacgcgcaag gtgaagatcg aggacgcgcg ctcgctgctg gtgctgccgc tgctggcggc
      181 cgacgagccg gtcggcacgc tgacgctggt cgcgcgggcc gagaagcggt ttggcaagga
      241 cgtgcgagag atgctgtcgg tgattgccaa tcaggtggcg atcgcgctgg agaacggatt
      301 cctctatcgc aagatggaga cgatggcgac gaccgacggg ctgaccgggc tgaccaacca
      361 ccggacgttc cagcagcggt tcgccgacat gctggagcgc gcggcgcgcc acaaccacaa
      421 ggtcgcggtg ctgctgtgcg acgtcgacca cttcaagaag gtcaacgaca actacgggca
      481 cccggtcggc gacgaggtgc tgcgccgggt cgcgcgggtg ctgcaggagg tgccgcgcaa
      541 gatcgacatc ccggcgcgct acggcggcga ggagttcgcg gtgctgctcg acaacgtcga
      601 cgtcgcgcag gccaagcagg tcgccgagcg gatccggatc gagatctcca ggctggtgat
      661 cgagagcgag aaggggccgc tgtcggtgac cgagtcgatc ggggtcgcgg cgttccccga
      721 cgacgggcgc gaccgcgcga cgctgatcga gcgcgccgac ctggcgctgt accacgccaa
      781 gcacaccggc cggaaccgcg tcgtgacgtg ggccgaggcg caggccgcga agggcggcca
      841 cgcccagagt gcccagagcg cgaagaaggc gagctgacgc ggtgcgcgcc ggatcggctg
      901 cacccgtgcc tccgaggcga ttcgtcgcag ccaggcgatg cgccggcggt cgccgcctgg
      961 ccccgggctt tccggagcgc gccgctgccc gcggtgatgt gtcgtcgcat cggcggtgga
     1021 caggtcgcga tccggcctta gattgtggtc aatgcacctc cgggtgatcg cgctgttcgt
     1081 cgcggtcgtc gtcgcttccg gtgccgccct ggcggtgatg gtcagcgcgt caccggcggt
     1141 ggctgcgtcg ccggacgtcg ccgggctggt cgacgacggc gcgctcgatc cggagccggc
     1201 gatcgccgca gcgccggtcg cgctcccggt gctggtgcgc cgcgagctgc gcccggtggt
     1261 cgcatcgccg ggggagctgc ccggtcgcct gcattccgcg tcggtgttcc gcccacctcg
     1321 ccggttcgcg cttccctgat ctcctgcgcg aaacgaaacc ctctcggagg tggcatgtcg
     1381 gaactcgtcg tgcccgcctg ggcctgggcc ctgctggcgg gcgtcatgtt gctgttgatc
     1441 gcgatcgatc tgttcgccca tcgcggcgat cggagcgatt cgcggcgcct cgcgctgatc
     1501 tggaccgtgg tgtggatcgc cgcggcggtc gtgttcggcg cgttcgtcgc ggtctggttc
     1561 ggcgcggccg cggccgagca gtacttcgcg gcgtacctgc tggagaagag tctgagcgtc
     1621 gataacctgt tcctgttcgt cgtggtgttc ggcgcgctcg acatcccggt gtccgagcag
     1681 cgccgggtgt tgacctgggg gatcctcggc gcgctggtca cgcgcggcgt gttcatcgcg
     1741 ctcggtgcgg cgatcctgca ccgctggcac gaggtcacgt acgtgttcgg cgcgatcctg
     1801 gtgatcacgg cgctcaagct gctgcgcggc ggcgagggca ggcccggccg gctgctgatc
     1861 tggctggagc gccacctgcc gtggacccgc gagcgcagcg gccatcgctt cctcgtgcgg
     1921 cgcgacgggc gctggctggc gacgccgctg ctcgtcgcgc tgctcgcgat cgagctgacc
     1981 gatgtgatgt tcgcgatcga ctcgatcccg gcggcgttcg cggtgtccga cgagccgttc
     2041 gtgatctaca gctcgaacgt gttcgcggtg ctcgggctgc gcgcgctgta cttggtgctg
     2101 gtcggcgcgc tcgccgatct gcgctacctg aagtacggcc tgtcggcggt gctcgcgttc
     2161 gcgggcagca agatgctggc ctcgccgtgg atccagatct cgccgatcgt gtcggtctcg
     2221 gtgatcgcgg tggtgatcgg catcgccgcg ggggccagcg tgtgggcggt gcggcgtgac
     2281 caccggcggg ggaggatggg agcaagccct tgatccgggc cgcgatccgg gggcctcgcg
     2341 caccaccgcc gggggcacgc tccgggccga gaccgcggcg gcgggtcgcg cgttgaacct
     2401 tgcgatgcgc tcgcccgctc gcatgctcga tcccacgggg ggccttgccc cccgccccgc
     2461 cggcagagcc ggcagggtcc ccacccccca tctcgctttg ctcgctgccc tcgtcgcttc
     2521 cactgcttgc atcgctgcct gcctggccgc ctg
//
LOCUS       DASVOR010001176         5018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_100114,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001176 DASVOR010000000
VERSION     DASVOR010001176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100114"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(449..1303)
                     /locus_tag="VF516_22345"
     CDS             complement(449..1303)
                     /locus_tag="VF516_22345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110497.1"
                     /translation="MPRAMTRTWYRVAVARPGGVPAVVAAAGEHMGVAVAVAERHAPG
                     SFAIAAEIADDSQIPLGESLGKSTVVELGGAPELPVFRWPVGVLPQLSTAGAVRGARR
                     GWIRRPASDLQVIEAQTTADHLTDLFLGMIERLPSADNLEVRVLDHFEDAGCADIWLT
                     SRVDARRILRLLDDHDEELLGNGHLELSVYLRAQRATLRLTEHKTVVWLASDRALGAD
                     VARWLRELDVPEVDALVTVQDAPHFHYRPARSRDRKRLGEALYRERLRRVDTLRPPPG
                     AVRAPRER"
     gene            complement(1330..2520)
                     /locus_tag="VF516_22350"
     CDS             complement(1330..2520)
                     /locus_tag="VF516_22350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110498.1"
                     /translation="MTRAAPAPLAACAAWVCACSTPTPQIVLGLAAPPVCPFDTCAML
                     LLPCDAVMSIRIVEPGDVSRRYLDQCVTVPRNKGNNVCALNMIDLEDKPLPVRDLAVQ
                     IAVFPLSAVQVDPDSGALTCPEVAYSANGLPITQAKAPVYGRQAFYHPGDSIVDVTLG
                     CTDLSAIQAGDSCSNPSAGAATATVLGFDTQVPVPVGPGGIADELFVWSGEPHIFDGS
                     YVLPPPDLVEMKLDDNGTPHWTAKAAQTFDGYACVEVLENAAQAVATLHCAPAAEAAA
                     ELTGYWISDRVLQPILKTAASVLGSSGLPGPGLTIGVVVDASSARGVQNVAVQINGPG
                     HITYLSGDGTIGRDATFASGVFVSDDVPFGTTFFAPGARPAIGGLVAGKATIVVLTMG
                     SPTH"
     gene            complement(2517..3602)
                     /locus_tag="VF516_22355"
     CDS             complement(2517..3602)
                     /locus_tag="VF516_22355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110499.1"
                     /translation="MREVAYKLLELLASHAELAPPAVSDGAALVDKLPPDDELRIADA
                     RRKVRSAAQNLTQRSFREAAIDAVEGQEALWRVTPRAAVLTLYADLELALGQSRLGEQ
                     KDAEAREAFALTLRLDPDRTLDERHYVPEVIQAFEAARAVRPELGKIVVRGIGRVWID
                     GEEVGEAPGEFPAPLGRHVVWLTGLMRETAGKEVVVTATRPGDATILDGPLTRPQKVV
                     RYRTVLADAQDPAKRAEAMQALAEFVNIHDAILLSAVNGRILWQTWCDRGPGFAAIRE
                     LPRDRPGEILKSLVPPHPVDLAGKATRLPALPPPPWYRRRTVQLGIAATVTAAVIGGY
                     FWTHYHEPDRPWNPNITGFGTGSGQGP"
     gene            3924..>5018
                     /locus_tag="VF516_22360"
     CDS             3924..>5018
                     /locus_tag="VF516_22360"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110500.1"
                     /translation="MLDRIAVGGMAEVFLAKAYGAHGFEKTLAIKRILPELASDPEFA
                     ARFIAEAKVAVRLSHANVVQVFDFGRIGESLFIAMEFVDGLDLAALLRRFKEERRQVP
                     LSAAFHVAIEIVRALDFAHSHNVVHRDVSPSNILISRAGEVKIADFGIAIAPSSHRGG
                     GAGPRKVMGKWRYMSPEQTRGDALDTRSDLFSAASVMFELFTGEKLFPGDEAEDIIRN
                     IESMAIPRMTAIRPGLPSRLDDVLGIALARKPIDRPSRPAAILRSLIELSYESSIMAT
                     ALDVAEAVATVLPITHHPGRGALDDVIRRQLADQSVARRTAVTDGKPPSTEVADPEPT
                     STGLFRKTDSDGLSRLEEAGEPAVATRPARA"
BASE COUNT          713 a         1822 c         1789 g          694 t
ORIGIN      
        1 ccgcgggtcg gcgtcgcgta cgccggcgcg tgggcgaacc gaccgctgcg gttctggtgg
       61 cgcgatcacc ccgcggtgtc gcggcccgcg gcggcgccgc gccggccctg acgctgtgtc
      121 gatcaaagcg ttcgatctgg ggtcccccac cttgaggttg acctcgtcgt tgactccctc
      181 gtgggccttg acggcgacgt aggacgtcaa ccccaccgtc gacctcgacc ttgcccatct
      241 ccgtcattct cgaggacgat tcctcgcaaa tgacccgggg ttcacgtcca agggggacgg
      301 cgggctcaac gactacgtcg ccctcaaggt caacgccctg cgacaacgtc gaggtcaacc
      361 tcaacggcct cggactcagc gcgagctcaa ttttctacac atgttgagcc ggcgagtctt
      421 cgagccggcg atctcctgca ctcgccggtc agcgctcgcg gggcgcgcgg accgcgccgg
      481 gcggcggccg cagggtgtcg acccgccgca gccgctcgcg atacagcgcc tcgccgagcc
      541 gcttgcggtc gcggctgcgc gccggccggt agtgaaagtg cggtgcatcc tggaccgtga
      601 ccagcgcgtc gacctcgggg acgtcgagct cgcggagcca gcgcgcgacg tcggccccga
      661 gcgcgcggtc gctcgcgagc cacaccacgg tcttgtgctc ggtgagccgc agcgtggcgc
      721 gctgcgcgcg caggtagacc gacagctcga ggtggccgtt gccgagcagc tcctcgtcgt
      781 gatcgtcgag caggcgcagg atccggcgcg cgtcgacccg cgaagtcaac cagatgtcgg
      841 cgcaccccgc gtcctcgaag tgatcgagca cgcggacctc gaggttgtcc gcgctcggca
      901 gccgctcgat catcccgagg aacaggtcgg tcaggtggtc cgcggtggtc tgggcctcga
      961 tgacctgcag gtcggaggcc gggcggcgga tccagccccg ccgcgcgccg cgcaccgcgc
     1021 ccgccgtcga cagctggggc agcacgccga ccggccagcg gaacaccggc agctcgggcg
     1081 cgccgccgag ctcgaccacc gtcgacttgc cgagcgactc gccgagcggg atctgcgagt
     1141 cgtcggcgat ctcggccgcg atcgcgaagc tgccgggggc gtggcgctcg gcgaccgcca
     1201 ccgcgacgcc catgtgctcg ccggcggccg ccacgacggc cgggacgccc ccggggcgag
     1261 ccacggcgac gcgataccag gtccgggtca tggcgcgagg cactcctgca ccgtaccgca
     1321 cggacacggt cagtgcgtcg gcgaccccat ggtcaggacg acgatcgtgg ccttgcccgc
     1381 gaccaggccg ccgatcgcgg gccgcgcccc cggcgcgaag aacgtcgtgc cgaacggcac
     1441 gtcgtcggac acgaacacgc cgctggcgaa cgtcgcgtcc ctgccgatgg tgccgtcccc
     1501 cgacaggtac gtgatgtggc ccgggccgtt gatctgcacc gcgacgttct ggacgccgcg
     1561 cgcggaggac gcgtcgacca ccacgccgat cgtgagcccg gggccgggca gccccgagct
     1621 tccgagcacc gacgccgccg tcttcaggat cggctgcagg actcgatcgg agatccagta
     1681 gccggtgagc tcggccgccg cttccgccgc cggcgcgcag tgcagcgtgg cgacggcctg
     1741 cgcggcgttc tcgagcacct cgacgcaggc gtagccatcg aacgtctgcg ccgccttggc
     1801 ggtccagtgc ggcgtgccgt tgtcgtcgag tttcatctcg accaggtccg gcggggggag
     1861 cacgtagctg ccgtcgaaga tgtgaggctc gccggaccac acgaacagct catcggcgat
     1921 tccacccggc ccgacgggca ccggcacctg ggtgtcgaag ccgagcaccg tcgcggtcgc
     1981 cgcgccggcg gacgggttgc tgcacgagtc accggcctgg atcgccgaca gatcggtgca
     2041 gccgagcgtg acgtcgacga tggagtcgcc ggggtggtag aacgcctggc ggccgtacac
     2101 gggagccttc gcctgcgtga tcgggagccc gttcgcgctg tacgcgacct ccgggcacgt
     2161 cagcgcgccg ctgtccgggt cgacctgcac cgccgatagt gggaacaccg cgatctggac
     2221 cgcgagatcg cgcaccggga gcggcttgtc ttcgagatcg atcatgttca gcgcgcacac
     2281 gttgttcccc ttgttccggg gcaccgtcac gcactggtcg aggtagcgcc tggacacgtc
     2341 gcccggctcg acgatccgga tgctcatcac cgcgtcacac ggcaacaaga gcatggcgca
     2401 cgtgtcaaat ggacagaccg gcggcgcggc gaggccgagc acgatctgcg gcgtcggggt
     2461 gctgcacgcg cagacccagg ccgcgcacgc cgcgagggga gcgggggccg cgcgcgtcat
     2521 ggcccctgac cggagccggt cccgaacccg gtgatgttgg ggttccacgg acgatccggc
     2581 tcgtggtaat gcgtccagaa gtagccgccg atcaccgcgg cggtgacggt cgcggcgatc
     2641 ccgagctgca ccgtgcggcg gcggtaccac ggcggcggcg gcagcgccgg caggcgggtg
     2701 gctttccccg cgagatcgac cgggtgcggc gggaccagcg acttcaggat ctcgcccggc
     2761 cgatcgcgag ggagctcgcg gatcgcggcg aaccccggcc cgcggtcgca ccaggtctgc
     2821 cacaggatcc tgccgttgac cgcggacagc aggatcgcgt cgtggatgtt gacgaactcg
     2881 gccagcgcct gcatcgcctc ggcgcgcttc gccgggtcct gggcgtccgc gagcacggtc
     2941 cggtagcgaa ccaccttctg cggccgggtc agcggcccgt cgaggatcgt cgcgtcgccc
     3001 ggccgcgtcg cggtgaccac gacctccttg cccgcggtct cgcgcatcag cccggtgagc
     3061 cacaccacgt ggcggccgag cggcgcgggg aactcgccgg gcgcctcgcc gacctcctcg
     3121 ccgtcgatcc agacccggcc gatgccgcgc acgacgatct tgccgagctc ggggcggacc
     3181 gcacgggccg cctcgaacgc ctggatcacc tcgggaacgt agtgccgctc gtcgagcgtg
     3241 cggtccggat cgagccggag ggtcagcgcg aacgcctcgc gcgcctcggc gtccttctgc
     3301 tcgccgagcc gcgactggcc gagcgccagc tcgagatccg cgtacagcgt gagcacggcg
     3361 gcgcgcggcg tgactcgcca cagcgcctcc tggccctcga cggcgtcgat cgccgcctcg
     3421 cggaagctgc gctgggtcag gttctgggcc gccgaccgca ccttgcgccg cgcgtcggcg
     3481 atccgcagct cgtcgtcggg cggcagcttg tcgaccagcg cggcgccgtc ggacaccgcc
     3541 gggggcgcga gctcggcgtg cgaggcgagt aactccagca gcttgtacgc cacctcacgc
     3601 accgcctgat cgttagtcag gtcgatcaca gcgaccgggt ggcgggagat cgccgccggg
     3661 ctagtgcccg cttctgccag ggccgcaggc gccggcagca ccggcgcgac gcgcggctcg
     3721 gcgcgcacgg acgcgccgag cgctgcgaag acgcacgagc acaggatcaa cgttcgcgac
     3781 gccatcccgg gcgacattcc cgtactatag gatacctggc gcgcgaggcc cactgaagtc
     3841 atcgtctccc agcgacgcgg atcgcgcgcc tgggacgccg cagtatggaa gagcccctac
     3901 agaaatcgct ggaacgctac gacgtgctgg accgcatcgc ggtcggcggc atggcggagg
     3961 tgttcctcgc gaaggcgtac ggggcgcacg gcttcgagaa gacgctcgcg atcaagcgca
     4021 tcctgcccga gctcgccagc gatcccgagt tcgccgcgcg gttcatcgcc gaggccaagg
     4081 tcgcggtccg gctgtcgcac gccaacgtcg tccaggtgtt cgacttcggc cggatcggcg
     4141 agtcgctgtt catcgcgatg gagttcgtcg acgggctcga tctcgccgcg ctgctgcgcc
     4201 ggttcaagga ggagcgccgc caggtcccgc tgtcggcggc gttccacgtc gcgatcgaga
     4261 tcgtccgcgc gctcgacttc gcgcacagcc acaacgtcgt gcaccgcgac gtgtcgccgt
     4321 cgaacatcct gatctcgcgc gccggcgagg tgaagatcgc cgacttcggc atcgcgatcg
     4381 cgccttcgtc gcaccgcggc ggcggcgccg ggccgcgcaa ggtgatgggc aagtggcgct
     4441 acatgtcgcc cgagcagacc cgcggcgacg cgctcgacac ccgcagcgac ctgttctcgg
     4501 cggcgtcggt gatgttcgag ctgttcaccg gcgagaagct gttccccggg gacgaggccg
     4561 aggacatcat ccgcaacatc gagtcgatgg cgatccccag gatgacggcg atccggcccg
     4621 gcctgccgtc gaggctcgac gacgtcctcg ggatcgccct ggcgcgcaag ccgatcgacc
     4681 ggccgtcgcg cccggcggcg atcctgcgct cgctgatcga gctgtcgtac gagtcctcga
     4741 tcatggcgac cgcgctcgac gtcgccgagg cggtcgcgac tgtcctgccg atcacgcacc
     4801 atcccggccg gggcgcgctc gacgacgtga tccgccgcca gctggccgac cagtcggtcg
     4861 cgcgccgtac cgcggtcacc gacggcaagc cgccgtcgac cgaggtcgcc gatcccgagc
     4921 cgacctcgac ggggctgttc cgcaagaccg acagcgacgg cctgtcgcgg ctggaggagg
     4981 ccggcgagcc ggcggtcgcg acccggccgg cccgggcg
//
LOCUS       DASVOR010001177         2970 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_100117,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001177 DASVOR010000000
VERSION     DASVOR010001177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100117"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..896)
                     /locus_tag="VF516_22365"
     CDS             complement(108..896)
                     /locus_tag="VF516_22365"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEX8110501.1"
                     /translation="MNKKDTRDIQLSVELDASPEDVFRAVTEGTEVAKWLAPEARSTA
                     PEGGRKARIWISWGEGMSAEHEIEIFDPPKRVRHPSGKNGETKAPLYADWLIEAREGG
                     KTTLRLVHSGFSASADWDDDYDAHARGWKLMLQNLRHYFARHAHEPAVHLPFMSNVES
                     PRGSLWKTLLGKLGVSAPPKVGDPFRFTTPKGDVLTGVVDFVAETRDLALVVREYDDA
                     LLRFTLSGKADAQPTFVYGYVIVYGAERERASELVAAGTSAVSS"
     gene            complement(893..1504)
                     /locus_tag="VF516_22370"
     CDS             complement(893..1504)
                     /locus_tag="VF516_22370"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8110502.1"
                     /translation="MADAAVLLRPLRARILEVLAEPQSAAGIGRTLDVARQKVGYHLK
                     ELEKQGLVELVEEKKVGNCVERIVRATGRAYLVSAEALGSLAQGAAEATDRFSAAYVI
                     AVAARALREVTRLRQAADDAGKRLATLTLETEIRFATAADRAAFAEELAREVARLTAR
                     YHDESAPRGRVHRIVAFAYPKPAVSDQESPGTQPAPGPRGSPP"
     gene            complement(1895..2053)
                     /locus_tag="VF516_22375"
     CDS             complement(1895..2053)
                     /locus_tag="VF516_22375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110503.1"
                     /translation="MSDKTKKLPRAIRISRKLPLSSEKLVVLSGGEATEPAAKCTLLR
                     TGCPAHTC"
     gene            complement(2167..2700)
                     /locus_tag="VF516_22380"
     CDS             complement(2167..2700)
                     /locus_tag="VF516_22380"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8110504.1"
                     /translation="MTSSTPDKTPSALAIRFATPADVSTILHFIRSLARYEKLEHEVV
                     ADEAGLRATLFGPRPGAEVLLAELAGSAVGFALFFQSYSTFLGKPGLYLEDLFVEPAA
                     RGQGIGLALMSALARITVERGYGRFEWSVLDWNTPALDFYRALGALPQSDWTVQRIIG
                     APLERLARRWSGAIAIP"
BASE COUNT          426 a         1009 c         1079 g          456 t
ORIGIN      
        1 cgttctacgg cgagctcacc ggccggctgc gcgatcagcc gccggtcgtg ccggcggggc
       61 gccacgcgcg gcggatccgc cgcggccgcc ggatcgcgtc cggctagtca gctggacact
      121 gcgctggtgc cggctgcgac gagctcgctc gcgcgctcgc gttcagcgcc gtacacgatc
      181 acgtagccgt acacgaacgt gggctgggca tccgccttgc ctgacagcgt gaagcggagc
      241 agcgcatcgt cgtattcgcg cacgacgagc gcgaggtcgc gcgtctccgc cacgaaatcg
      301 accacgcccg tgaggacgtc gcctttcggc gtcgtgaatc ggaacgggtc gccgaccttc
      361 ggcggggccg aaacgccgag cttgccgagg agcgtcttcc agagcgaccc gcgcggggac
      421 tcgacattcg acatgaaggg gaggtgcacc gccggttcgt gcgcgtgtcg cgcgaagtaa
      481 tggcggaggt tctggagcat cagcttccag ccgcgcgcgt gcgcgtcgta atcgtcgtcc
      541 cagtcggcgc ttgcactgaa cccggaatga acgaggcgga gcgtggtctt gccgccttcg
      601 cgcgcttcga tcaaccagtc ggcatagagc ggcgccttcg tctcgccgtt cttgccggac
      661 ggatgacgga cccgcttcgg cgggtcgaag atctcgatct cgtgctcggc gctcatgccc
      721 tcgccccacg agatccagat cctggctttc ctcccgcctt ccggtgcggt ggagcgcgct
      781 tcgggcgcga gccatttcgc gacctcggtg ccttcggtga cggcgcggaa gacgtcttcg
      841 ggcgacgcat cgagctcgac agagagctgg atgtcgcggg tgtctttctt gttcatggtg
      901 gtgagcctcg tgggcctggt gctggttgtg ttcccggtga ttcctgatcg ctgactgcag
      961 gcttcgggta ggcgaaagca acgatgcgat gcacgcgtcc gcgcggagcg gactcgtcgt
     1021 ggtacctggc ggtgagccgc gcgacctcgc gcgcaagctc ctcggcgaag gcggcacgat
     1081 cggccgccgt cgcgaagcgg atctcggtct cgagcgtgag cgtcgcgagg cgcttgccgg
     1141 catcgtccgc cgcctggcgc aggcgcgtca cctcgcgaag cgcgcgcgcc gcgaccgcga
     1201 tgacgtatgc cgcggagaac cgatccgtcg cttccgccgc gccctgggcg agtgatccga
     1261 gcgcttcggc cgacacgaga taggcgcgcc cggtcgcgcg aacgatgcgc tcgacgcagt
     1321 tgccgacctt cttctcttcg acgagctcga cgagcccctg cttctcgagc tccttcaggt
     1381 ggtagccgac cttttgccgc gccacgtcga gcgtgcgccc gatgccggcc gcggactgcg
     1441 gctcggcgag cacctcgagg atgcgcgccc gaagcgggcg aagcagcacg gcggcgtcag
     1501 ccatgcacgc actttcatac cgaaaactta ttcggtcaag gaaaaatttt tgtcggatgc
     1561 gacagcgacg gcatcatctg cggcgagtag ggtcgctcgc cgcaagaccg gctccggctg
     1621 tccagccgga atcgaccgtc gatgttcccc tcgtggggcg atcgcgagac gtcaacgcga
     1681 acgcgccggg ggcgaccgcg agcaggatgg ggtcggacgc cgcgaccgtg atttggattg
     1741 gccgggcctt gcccgcacat acctcgcgac gctcgcgcag gacgggtggt gattcggcgg
     1801 gcgcggctcc gacacccatc cgcgcagcag cccccggcgc gtcgcgtgta ggcgctctcc
     1861 cgggcgttac gcgctacgcg ctacacactc ctcgctagca ggtgtgcgcg gggcagccgg
     1921 tgcgcagcag cgtgcacttg gcggcgggtt cggtggcctc gccgccggac agcacgacga
     1981 gcttctcgga cgacagcggg agcttgcgcg agatgcggat ggccctgggg agcttcttgg
     2041 tcttgtcgga catgggtacc tccacgacca gggtagctgc ggcgtccgcg cgccgacaat
     2101 cgcggcgtcg cgcggctgtg gggaagcgca cgcgtgggcc gaggtggcgc gccggtcgcg
     2161 gcgatctcac ggaatcgcga tcgcgcccga ccatcgccgc gcgagccgct cgagcggcgc
     2221 gccgatgatg cgctgcaccg tccagtcgct ctgcggcagc gcgccgagcg cgcggtagaa
     2281 gtcgagcgcc ggggtgttcc agtcgagcac cgaccactcg aaccggccgt agccgcgctc
     2341 gaccgtgatc cgcgccagcg cggacatcag ggccaggccg atgccctggc cgcgcgccgc
     2401 cggctcgacg aacaggtcct cgaggtacag ccccggcttg ccgaggaacg tcgagtagct
     2461 ctggaagaac agcgcgaagc cgaccgcgct gccggcgagc tcggcgagca ggacctcggc
     2521 gccgggccgc ggcccgaaca gcgtcgcgcg cagcccggcc tcgtcggcga cgacctcgtg
     2581 ctcgagcttc tcgtagcggg cgagcgagcg gatgaagtgc aggatcgtcg aaacgtcggc
     2641 gggcgtggcg aaccggatcg cgagcgcgct cggggtcttg tcgggggtgg aggacgtcat
     2701 gcgagctccg gaacggcggg ccgccggacg atacgcgagt ccggccggcg atgcgcggat
     2761 ggcacgccgc gatcgccgcg ctaccattcc gtgccatggc ggtgatcgcg tgcgatgcct
     2821 gcggcggcaa gatgaacgac ggcctggcgg tgtgcccgca ctgcggcgcg cgccgtgcac
     2881 tgaccgagcg gacccggctg tcgggcgacg agatccgcgc cctgctcgcg accgatcgga
     2941 ccgctcgcga cggcgagccg cgcggcctgg
//
LOCUS       DASVOR010001178         3013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362700,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001178 DASVOR010000000
VERSION     DASVOR010001178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362700"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VF516_22385"
     CDS             complement(<1..1267)
                     /locus_tag="VF516_22385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110505.1"
                     /translation="MPRSREVIDERFTIEQLAGFGGMGQIYRARDRRSGGLVALKVMH
                     DAERLELERFAREAQVLATLSHPGIVRYIDSGITAEGEPYLVMEWLSGETLSTRLQRT
                     QLTLSEALALGRRVASALSAIHRGGIVHRDIKPSNLFLRDGAIDKVTLIDFGMARRPL
                     ADPRLTVTGTMLGTPGYIAPEQVDHFSTLDGRADIFSLGCVLYRCISGRAPFRGPDAL
                     RMLLNGTPRMLPRLREFRPSIPRELDDLVARMLSRSPEDRPASGDAVAAELLAIEESE
                     PSPLSEARSGPRSVLSEIMAVERRLMSLVVVRLPERTDGSLTIAERAAHERALHDLIE
                     RHRGKLEVLADGLLLVILSNADAAIDLARRAARCALALRNALGDGAAAVVTGREIPGP
                     SLRDSELLHRAVDLLDGARGTSMVRLDEMT"
     gene            complement(1630..>3013)
                     /locus_tag="VF516_22390"
     CDS             complement(1630..>3013)
                     /locus_tag="VF516_22390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110506.1"
                     /translation="SWVSWVEASRFDPAVAYAAFDRHTFGDMTPWVFRTTDYGKTWTR
                     IVGPDQGVRGYAHTIKEDTVKKDLLFVGTELGLWISPDGGARWAQFRGGEFPDGVAVR
                     EVQVHPRDGDLVLATHGRGIWIVDDLTPLRTLADDVLQKSTAFLPIRDVQQRMPARGG
                     WSEGDATFSGDNAPGGAAITYYLRSRHVYGPIKLEVLDAGGKLVDTITPGKRRGINRV
                     EWNMRVKPPKVPRAAQVAFSASQGPRVPPGTYTVRLTRGTEVIETKLAIGLDRRAPYS
                     VADRKAQVDAVMKAHGLFGDMTKLATRIDGAREAVRDRLKGLPGTDPLAGKLRALGDK
                     LEAARKQIVATTEGGAITGEERIREHLDQLYGALDRWEGRPAKYKLDRIEVLRRELGD
                     VTATVDAIVGKDARALDDELKQHQIAPLPQISALDRGGPLDALALQCAASRGASCGDD
                     DAAVATDERD"
BASE COUNT          408 a         1094 c         1053 g          458 t
ORIGIN      
        1 cggtcatctc gtcgaggcgg accatcgacg ttccgcgcgc cccgtccagc aggtcgaccg
       61 cgcggtgcag gagctcgctg tcgcgcaggc tcgggcccgg gatctcccgg ccggtgacga
      121 ccgccgccgc gccgtcgccg agcgcgttcc ggagcgcgag tgcgcagcgt gccgcgcgcc
      181 gcgcgaggtc gatcgcggcg tcggcgttcg acaggatcac gagcagcagc ccgtcggcga
      241 gcacctcgag cttgccgcga tggcgctcga tcaggtcgtg gagcgcccgc tcgtgcgccg
      301 cgcgctccgc gatcgtcagc gagccgtcgg tgcgctccgg caagcggacc acgaccagcg
      361 acatcagccg gcgctccacc gccatgatct cggacagcac gctgcgcggt ccggatctgg
      421 cctccgacag cgggctgggc tcgctctcct cgatcgccag cagctcggcg gcgaccgcgt
      481 cgccgctggc cggccggtcc tcgggcgacc gcgacagcat gcgcgcgacc aggtcatcga
      541 gctcgcgcgg gatcgacggg cggaactcgc ggagccgggg cagcatccgc ggcgtcccgt
      601 tgagcagcat gcggagcgcg tcggggccgc ggaacggtgc gcgtccggag atgcagcggt
      661 acagcacgca gccgagcgag aagatgtcgg cccggccgtc cagcgtcgag aagtgatcga
      721 cctgctcggg cgcgatgtag cccggcgtgc cgagcatcgt gccggtgacc gtgagccggg
      781 gatcggcgag cggccggcgc gccatcccga agtcgatcag cgtgaccttg tcgatcgcgc
      841 cgtcgcgcag gaacaggttg ctcggcttga tgtcgcggtg cacgatgcca ccgcggtgga
      901 tcgcgctgag cgccgacgcc acacgccggc cgagcgcgag cgcctcggac agcgtcagct
      961 gcgtgcgctg gagccgcgtc gacagggtct ctcccgacag ccactccatc accaggtacg
     1021 gctcgccttc ggcggtgatt ccgctgtcga tgtaacggac gatgccggga tgcgaaagcg
     1081 tggcgagcac ctgcgcctcg cgcgcgaacc gctcgagctc caggcgctcg gcgtcgtgca
     1141 tcaccttgag cgccaccagt ccgcccgacc tccgatcgcg ggcccgatag atctggccca
     1201 tcccaccgaa ccccgccaac tgctcgatcg tgaaccgttc gtcgatgacc tctctgctcc
     1261 gcggcatccg ctccatcttt cgattgacca ctcctacatc cgacctcgat cccaccacgg
     1321 tcggacgttc cacaatcaga gcggaatcgc agcagaacct caagaggggc gtttggccca
     1381 catcctcgtc tgaaggtgcg ttcagcccgg tttcggttag cttcgatgcc aagcccatgt
     1441 ctggcgctgt ttccggcacc ggacccaggt tagcggcgca agctctgcgg tgcggttacg
     1501 ggccgggtag cgaggcccgc cgatcgttca ccgggcgtga gacgggatca tgagacggag
     1561 ctgggagccg gagccgggag ccgggagccc ggatcgtgag ccgagccaaa cccggtcagg
     1621 aattgacttt caatcgcgct cgtcggtcgc gaccgccgcg tcgtcgtcgc cacagctcgc
     1681 gccccggctg gccgcgcact gcagcgcgag ggcgtcgagc ggcccgccgc ggtcgagcgc
     1741 gctgatctgg ggcagcggcg cgatctggtg ctgcttgagc tcgtcgtcga gtgcgcgcgc
     1801 gtccttgccg acgatcgcgt cgaccgtggc ggtcacgtcg ccgagctcgc ggcgcaggac
     1861 ctcgatccgg tccagcttgt acttggccgg ccggccctcc cagcgatcga gcgcgccgta
     1921 cagctggtcg aggtgctcgc ggatccgctc ttcaccggtg attgcgccac cttccgtcgt
     1981 tgcgacgatc tgcttgcgcg cggcctccag cttgtcgccc agcgcgcgca gcttgccggc
     2041 cagcgggtcg gttccgggca gccccttcag ccggtcgcgc accgcctcgc gcgcgccgtc
     2101 gatccgggtc gcgagcttgg tcatgtcgcc gaacaggccg tgcgccttca tcaccgcgtc
     2161 gacctgcgcc ttgcggtccg ccacgctgta cggcgcgcgg cgatcgagcc cgatcgcgag
     2221 cttggtctcg atcacctcgg tgccgcgggt cagccgcacc gtgtacgtgc ccggcgggac
     2281 ccgcgggccc tgcgacgccg agaacgcgac ctgtgcggcg cgcggcacct tgggcggctt
     2341 gacccgcatg ttccactcga cccggttgat cccgcgccgc ttgcccgggg tgatcgtgtc
     2401 gaccagcttg ccgccggcgt cgagcacctc gagcttgatc gggccgtaga cgtggcgcga
     2461 ccggaggtag taggtgatcg ccgcgccgcc cggcgcgttg tcgccgctga acgtggcgtc
     2521 gccctccgac cagccgccgc gcgccggcat ccgctgctgg acgtcgcgga tcggcaggaa
     2581 cgcggtcgac ttctgcagca cgtcgtcggc gagcgtgcgc agcggggtca gatcgtcgac
     2641 gatccagatc ccgcggccgt gggtcgcgag caccaggtca ccgtcgcgcg ggtggacctg
     2701 gacctcgcgg accgcgacgc cgtcggggaa ctcgccgccg cggaactgtg cccaccgcgc
     2761 gccgccgtcg ggcgagatcc acagcccgag ctcggtgccg acgaacagca ggtccttctt
     2821 gaccgtgtcc tccttgatcg tgtgggcgta gccgcgcacg ccctgatccg gcccgacgat
     2881 ccgggtccag gtcttgccgt agtcggtggt ccggaacacc cacggcgtca tgtcgccgaa
     2941 cgtgtggcgg tcgaacgccg cgtaggcgac cgccgggtcg aaccggctgg cctcgaccca
     3001 gctgacccaa gac
//
LOCUS       DASVOR010001179         6911 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_479598,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001179 DASVOR010000000
VERSION     DASVOR010001179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6911
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_479598"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="VF516_22395"
     CDS             <1..714
                     /locus_tag="VF516_22395"
                     /inference="COORDINATES: protein motif:HMM:NF028495.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HEX8110507.1"
                     /translation="VGGGGSDDLTFTYADGTVAQLHFEGMKNPAPAWANGIGRMIDPI
                     TGRDHGTQFYYNRRNMAAYGTIQRPGKRRVHVVGIGWYDREFGSVIGTRGTQDNNVNW
                     RWLSLHLSDDTDYMMWDMYANDTGNNLLHYLNEIGQAPTCAEKTITTYTFTPSSSTVF
                     NDGPPASRLNLGGHLSVPEEEIEIDIHPLRSNQIVTSGGLFSPFFEGAFVVSGTKHGR
                     HVTGTGYFEEFVTPRGCCQ"
     gene            1141..1389
                     /locus_tag="VF516_22400"
     CDS             1141..1389
                     /locus_tag="VF516_22400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110508.1"
                     /translation="MIRITFALASLAALALLLPVLGMVLLGLLTLVVPMLLVLTPVLV
                     VLSVGFLVDRLRGKREPAGVPGAEPMPIGAPALHAPAP"
     gene            complement(1432..2355)
                     /locus_tag="VF516_22405"
     CDS             complement(1432..2355)
                     /locus_tag="VF516_22405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8110509.1"
                     /translation="MIALALRPFGTTGLSVSALGLGAGEIGDPALDDREVEALVRAAV
                     ELGVTLFDTARSYGRSEERLGRFLAPVRDRVVLSTKVGYGVDGVPDWTGECVRRGVDR
                     ALGQLATDRIDIVHLHSCPADVLARGDAIDALIDARRAGKIRCAAYSGDNDALDWAAA
                     SGRFDSLQLSWNLCDQRAAAVIERAHAAGLGVIAKRPLANAPWRFAERPTGHEAELYW
                     ERWQALGIDSGQMVWPGQPGWAGALGWDELAARFAAHTPGVSSAITGTRSLAHLAHNA
                     ELVKRGALPPAVFAAVRARWRVVGADWPARI"
     gene            complement(2440..3744)
                     /locus_tag="VF516_22410"
     CDS             complement(2440..3744)
                     /locus_tag="VF516_22410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110510.1"
                     /translation="MAIAIGGLGLAIGALAACPGPEEDVALPPPSVIPNGSCGLPGSV
                     IWEARGPTRVPGGPAHSPDISWMKLPVGFCAHVYATVPHVRQVKVAPSGELFAASPSQ
                     STAGGGDGGLGAIVVLPDDDHDGTSDGPLRFVDHVAETQGMAFSPDGFLYYQDAAAVM
                     RVPYRPGDRAPSSPGAMMIDMASIVPEQPTLHWPKAMDVADDGSLYVMNGGEQGDQCQ
                     SPLPVRGAIVHVASPAGGSPVGTVIARGFRNPIAVRCHPGKGRCFALELAKDGTGDEG
                     GREKLVPVRDGANDDWGFPCCATTGIPYPDVSGSAPDCSGVTDETVGFIIGNTPFGLE
                     FAPSTWPAPFRSAAIVALHGRVGSYAGARVIAIPTDAATGEPIKSSNLSGDGGFLELA
                     SGWDDGRQDHGRPAAVGFAADGRLFLGDDIAGLIVWIAPVAP"
     gene            4400..>6911
                     /locus_tag="VF516_22415"
     CDS             4400..>6911
                     /locus_tag="VF516_22415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110511.1"
                     /translation="MGLRLVPRSACAIGLVLGAASCSDPPPDEAATSQKDSSGLHFPG
                     ELRDTNAPPQSTSAPFGAHLQYFGGRVVSNIQVVQVLYGAGSYIPQVTSTGTPSMASF
                     YQGVLNSPYVDWLSEYNTPTQTIGRGSFLTQTTILPAPEHNGTFIDDTQIQAELSAQI
                     QAGNLPAPSHDAQGNNNTYYAVFFPHGKTITLQGFSSCSFFCAYHGTIANAGGAGEIA
                     YGVHPDFQPGSGCEFGCGAAATQFGNYTQVASHELVETMTDPEVGLAPFIGPPLAWYD
                     PSFGEIGDICNDQNGHIVGSDGVTYDVQNEFSNSLNNCVVTNPLASPIVVGPPTENCR
                     GTTSLSTVTVLGGSGRFTSDVTLSLAGVSPAPPAGGAITATFDPNPVPTPSASGTPST
                     MRVTTSAATPPGTYALTVQGATPALTKTQTATLTVRAAVASAPTLVSPASGADGVLPL
                     PTFAWSSVDQSTLYTLDLFDGSDCSGDPIHTFETTSTSFAMPKDQPLPLFHAFSWRVT
                     ASNACGGNAGVSACSHFRTESCSDPHETITNGGFENGLTAWTVDASSPPPVVTGDQPH
                     TGANAVRLGDMSGFFVTGGDSQVSQTVTLAPASTPTLSFWEWPRSDDSIFFDQQYVRV
                     TPISPPGPTVVLMNELRNDRTYIHRTFDLSQFAGMTVKVTFGVHEDFVEVTGMFIDDV
                     SITSPNCGPPDFVVHVTPPNPEEVCAGASLAMGVAVDSVNGPNFTSPVTLSAGRLPPG
                     ATASFAKNPINPGESTTLTLTTARPTAGDTFDIDVTGVAVTPPPDGARTVTTTLRIDP
                     NAPGAPEIASPRVGEINVPRRPTLSWTAPFVPESAVATS"
BASE COUNT         1076 a         2491 c         2302 g         1042 t
ORIGIN      
        1 gtcggcggcg gtggcagcga cgacctcacg ttcacctacg ccgacggaac ggtggcacag
       61 ctccacttcg aggggatgaa gaatcccgca ccggcgtggg ccaacgggat cggccggatg
      121 atcgacccga tcaccgggcg cgaccacggg acgcagttct actacaaccg ccgcaacatg
      181 gccgcgtacg gcacgatcca gcggccgggc aagcggcggg tccatgtcgt cgggatcggc
      241 tggtacgatc gcgagtttgg ctccgtgatc ggcacgcggg gcacccagga caacaacgtg
      301 aactggcgct ggctatcgct gcacctgtcc gacgacaccg actacatgat gtgggacatg
      361 tacgcgaacg acaccggcaa caacttgctt cactacctga acgagatcgg gcaggccccc
      421 acgtgcgccg agaagacgat caccacgtac acgttcaccc cgtcgagctc gacggtgttc
      481 aacgacgggc cgccggcgag ccggctgaac ctcggaggcc acctgtcggt tccggaggaa
      541 gagatcgaga tcgacatcca tccgctcagg agcaaccaga tcgtcacgtc cggcgggttg
      601 ttcagcccgt tcttcgaggg agcgttcgtc gtctcgggga cgaagcacgg ccggcacgtc
      661 accgggaccg gttacttcga agagttcgtg acgccgcgcg gctgctgcca gtagtcaccg
      721 ccccgggccg gtcgcgtcta tcccctatgt gaaacaccgg tcggcagcga gcccgtcgtt
      781 gctcgccgtg ctcgggcgct cgggatcccg tgcacgcggc tggcgctcgc gtcggcgcgg
      841 gacccgccga atggccgcgc tctggttcga cccttctcgt cgggtgacga cgtggaacac
      901 tccgtctcat cagttttctg agttttgggt gatctgtcat gcgatctctt gtgctatgac
      961 gcgtttatga tattgtaatt tataaatttt ccaagaatca catcgtatca gatttctgat
     1021 ggaaacgcgt cgatactgtc ggcttcgggg gaaagcagat acccatggtt gcgttccgaa
     1081 cccacgcagc acccgccgaa ctgcccgaaa ggtcgctcca tgaacacgaa gacgtcatct
     1141 gtgatcagaa tcacgttcgc cctcgcatcg ctcgctgcgc tggcgctcct gcttccggtc
     1201 ctggggatgg tcttgctcgg tctcctgacg ctggtcgtgc cgatgctgct cgtgctgacc
     1261 cccgtgctcg tggtgctctc ggtgggcttc ctggtcgacc ggctgcgcgg caagcgcgag
     1321 cccgccggcg ttcccggagc cgagcccatg cccatcggcg cgcctgcgct tcatgccccc
     1381 gcaccctgag cgagctcggc cggcgcccgg atctccctct cggtctcgca gctagatccg
     1441 cgcgggccag tcagccccga cgacgcgcca ccgggcgcgg accgcggcga acacggccgg
     1501 cggcagtgcg ccccgcttca ccagctccgc gttgtgggcg aggtgagcga ggcttcgcgt
     1561 cccggtgatc gccgacgaca cgccgggagt atgcgcggcg aaccgcgcgg cgagctcgtc
     1621 ccatcccagc gcaccggccc agcctggctg accgggccag accatctgac cggaatcgat
     1681 gcccagcgcc tgccagcgct cccagtacag ctcggcctcg tgacccgtgg ggcgctcggc
     1741 gaaccgccac ggcgcgttgg ccagcgggcg cttggcgatg acgccgaggc cggcggcatg
     1801 ggcgcgctcg atcaccgccg ccgcgcgctg gtcgcacagg ttccacgaca gctgcagcga
     1861 gtcgaaccgg ccgctcgccg cggcccagtc gagcgcatcg ttgtcgcccg agtaggccgc
     1921 gcaccggatc ttgccggcgc gccgggcgtc gatcagggcg tcgatcgcgt cgccgcgcgc
     1981 cagcacgtcg gccgggcacg agtggaggtg gacgatgtcg atgcggtcgg tggcgagctg
     2041 cccgagcgcg cgatcgacgc cgcgccggac gcactcgccc gtccagtcgg ggaccccgtc
     2101 gacgccgtag ccgaccttgg tcgacagcac gacccggtcg cgcaccggcg ccaggaaccg
     2161 gccgagccgc tcctccgacc ggccgtagct gcgcgcggtg tcgaacagcg tcacgccgag
     2221 ctcgaccgca gcgcgcacca gcgcctcgac ctcgcgatcg tcgagcgcgg ggtcgccgat
     2281 ctcaccggcg ccgaggccga gcgccgagac cgagagcccc gttgttccga acggacgcag
     2341 cgcgagcgcg atcacggcac tcgcgtagca tgttccgagt cgcggtggaa acgcgccccc
     2401 ggttggcgcg gggctgaact gaccgctcgc cggtgacgct cagggcgcga ccggcgcgat
     2461 ccagacgatc agcccggcga tgtcgtcgcc gaggaacagc cgcccgtcgg cggcaaaccc
     2521 gaccgcggcg gggcgcccgt gatcctgccg gccgtcgtcc cagcccgacg cgagctcgag
     2581 gaagccaccg tcgcccgaga ggttcgagct cttgatcggc tcgccggtcg ccgcgtcggt
     2641 cgggatcgcg atcacgcgag cgccggcata gctgcccacc cggccgtgca gcgcgacgat
     2701 cgccgcgctc cggaacgggg ccggccaggt gctcggtgcg aactcgaggc cgaacggcgt
     2761 gttgccgatg atgaagccga cggtctcgtc cgtgacgccg ctgcagtcgg gcgccgagcc
     2821 ggagacatcc gggtacggga tgcccgtcgt tgcgcagcac gggaagcccc agtcgtcgtt
     2881 cgcgccgtcg cgcaccggga cgagcttctc ccgcccgccc tcgtcgcccg tgccatcctt
     2941 ggcgagctcc agcgcgaagc atcgtccctt gccgggatgg cagcggaccg cgatcgggtt
     3001 gcggaacccg cgcgcgatga ccgtgccgac cgggctgccg ccggccggcg acgcgacgtg
     3061 gacgatcgcg ccgcgcacgg gcagcggcga ctggcactgg tcgccctgct cgccgccgtt
     3121 catgacatag aggctgccat cgtctgccac gtccatcgcc ttgggccagt gcagcgtggg
     3181 ttgctcgggg acgatcgagg ccatgtcgat catcatcgcc cccgggctcg acggcgcgcg
     3241 gtcgccgggg cgatacggga cgcgcatcac cgccgccgcg tcctggtagt agaggaagcc
     3301 gtccggggag aatgccatgc cctgggtctc ggcgacatgg tcgacgaacc ggagcgggcc
     3361 gtcgctcgtt ccatcgtggt cgtcatccgg aagcaccacg atcgccccga gcccgccgtc
     3421 gccgccgccg gccgtgctct ggctgggcga cgcggcaaac agctccccgc tcggagcgac
     3481 cttcacctga cggacgtggg gcaccgtggc gtagacgtgg gcgcagaatc cgaccggcag
     3541 cttcatccac gagatgtccg ggctgtgggc gggtccgccc gggacccggg tcggcccgcg
     3601 cgcctcccag atgacggatc ccggcagacc gcaggacccg ttcgggatca cgctcggggg
     3661 aggcagcgcg acatcctcct cggggccggg gcaggcggcc agcgcgccga tcgccaagcc
     3721 gagcccaccg atcgcgatcg ccatgcgtgc gcagcaacga acgtctgcca tcacgcagac
     3781 actattgtcg gagcgccccc gggagcaagc ggcaagtacg gccggcgcgg gcaggtcgat
     3841 gcgcaggtca cggggtcgct gccggcaggt gacttcgcgg agacggggac cccggtgcgg
     3901 cagccggtcg ctcccgcgct acggccctcg cggagatcca ggccgcgcgg agatcttgtc
     3961 cggtcgttgt ggtttgcgac ccacgtctgt cgcctgtgat gtcgatgaca cagctccgac
     4021 actcccgatc gatgcacgcg atctctgctg cgggcgcagc acgaccgccg gccgcgcctc
     4081 gatcgataca gcctcagatc ccggcgcggg catcgccgcc tggcaatgac aggcaaggtg
     4141 aatccatcgc acatggcggg tattccatat cgagaaaatc ctgatagtgt ccatccgcca
     4201 gtatcgatgc gcctgatggg gaagggcggc ggcgacgtgc catggggcga ggtgcatccg
     4261 cggcctcggg ccggcggcgt ccgttcatcg ttctggtgat ctgactcgtc tgttgttcga
     4321 acgatcttgt ccggcgggtc cgagcgtgtc tcgagcccgc agagaaacat tctttcagtg
     4381 atgtcttgag gaggcgttca tgggtttacg gcttgttcca cgctccgcgt gcgccatcgg
     4441 cctcgtgctg ggcgcagcca gttgctcgga tccaccacca gacgaggccg cgacgtccca
     4501 gaaggacagc agcggcctcc actttcccgg cgagctccgc gacacgaatg cgccgccgca
     4561 gagcacgtcg gcgccgttcg gtgctcacct gcagtacttc ggcggccgcg tcgtgtccaa
     4621 catccaggtc gtccaggtgc tctacggcgc cggcagctac atcccgcagg tcaccagcac
     4681 ggggacgccg agcatggcgt cgttctacca gggtgtcctc aacagcccgt atgtcgactg
     4741 gctctccgag tacaacaccc ccacgcagac catcggccgc ggctcgttcc tgacccagac
     4801 gacgatcctc ccggccccgg agcacaacgg caccttcatc gacgacacgc agattcaagc
     4861 agagctatct gcgcagatcc aggccggcaa tctgccggcc cccagccacg acgcccaggg
     4921 caacaacaac acgtactacg cggtgttctt tccccacggc aagacgatca cgctgcaggg
     4981 gttctcgtcg tgctcgttct tctgcgcgta ccacgggacc atcgccaacg ccggcggcgc
     5041 cggtgagatc gcgtacggcg tgcaccccga cttccagccc ggatcgggct gcgagttcgg
     5101 ctgcggcgcg gccgcgaccc agttcggcaa ctacacgcag gtcgcgtcgc acgagctggt
     5161 cgagaccatg accgatcccg aggtcgggct ggccccgttc atcggcccgc cgctcgcctg
     5221 gtacgatccg tcgttcggcg agatcggcga catctgcaac gatcagaacg gccacatcgt
     5281 gggctcggac ggcgtgacct acgacgtgca gaacgagttc tccaactcgc tgaacaactg
     5341 cgtcgtcacc aacccgctcg cgagcccgat cgtcgtcggt ccgcccacgg agaactgccg
     5401 ggggaccacc tcgctgtcga ccgtgaccgt gctcggcggc tccggccggt tcacgagcga
     5461 cgtgacgctc agcctcgccg gcgtgagccc ggccccgccg gccggcggcg cgatcaccgc
     5521 gacgttcgat ccgaacccgg tcccgacccc gagcgccagc ggcacgccgt cgacgatgcg
     5581 ggtcacgacc agcgcggcga cgccgcccgg cacgtacgcg ctgaccgtgc agggcgcgac
     5641 cccggcgttg accaagaccc agaccgccac gctgacggtc cgcgcggcgg tggccagcgc
     5701 gcccacgctg gtgtccccgg ccagcggcgc cgacggcgtc cttccgctcc cgaccttcgc
     5761 ctggtcgtcg gtcgaccaga gcacgctcta cacgctcgac ctcttcgatg gcagcgattg
     5821 cagcggagat ccgatccaca cgttcgagac cacgagcacg tcgttcgcga tgccgaagga
     5881 ccagccgctg ccgctgttcc acgcgttctc ctggcgggtg accgcgagca acgcgtgcgg
     5941 cggcaacgca ggcgtctcgg cgtgctcgca cttccgcacc gaatcgtgct ccgatccgca
     6001 cgagacgatc accaacggcg gcttcgagaa cggcctcacc gcctggacgg tggacgcgag
     6061 ctctccgccg ccggtggtga ccggggacca gccccacacc ggcgccaacg cggtgcggct
     6121 cggcgacatg tccggattct tcgtgaccgg cggtgattcc caggtctcgc agaccgtgac
     6181 cctggccccg gccagcaccc cgacgctgag cttctgggag tggccgcggt ccgacgacag
     6241 catcttcttc gatcagcagt acgtgcgcgt gacgccgatc agcccgccgg gcccgacggt
     6301 cgtgctgatg aacgagctcc gcaacgatcg aacgtacatt caccgcacgt tcgatctgtc
     6361 gcagttcgcc ggcatgacgg tgaaggtcac gttcggcgtc cacgaggact tcgtcgaggt
     6421 gaccggcatg ttcatcgacg acgtcagcat cacgtccccg aactgcgggc cgccggactt
     6481 cgtcgtccac gtcacgccgc ccaacccgga ggaggtatgc gccggggcga gcctcgcgat
     6541 gggggtggcg gtcgactcgg tcaacggccc caacttcacc tcgccggtga cgctgagcgc
     6601 cggccggctc ccgccggggg ccaccgcgag cttcgcgaag aatccgatca acccgggcga
     6661 gtcgacgacg ctgacgttga ccacggcgcg gccgaccgcc ggcgatacgt tcgacatcga
     6721 cgtcacgggc gtcgcggtca cgccgccgcc cgacggcgcc aggaccgtca cgaccacgct
     6781 gcgcatcgac cccaacgcgc ccggtgctcc cgagatcgcg agcccgcgcg tgggcgagat
     6841 caacgtgccg cgccggccca cgctgtcgtg gaccgcgccg ttcgtgccgg agtccgcggt
     6901 cgcgacctcg t
//
LOCUS       DASVOR010001180         2377 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_12709,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001180 DASVOR010000000
VERSION     DASVOR010001180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12709"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VF516_22420"
     CDS             <1..318
                     /locus_tag="VF516_22420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110512.1"
                     /translation="LRLAQSRVAEAVAAAEDAVARCTTMGGCGMFRGAFVRLARAEAL
                     HASGAHDAARHAIADAQGRLLAIADRIGDPAYQHSFLHNVPENARTLTLVRSWLGETA
                     SGT"
     gene            997..>2377
                     /locus_tag="VF516_22425"
     CDS             997..>2377
                     /locus_tag="VF516_22425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8110513.1"
                     /translation="MAVCVLGLSAAPSCAPPVDADRAAGVDEPGRADAVVDRQTAATR
                     PASASSSNTSSFALFESGQVRPLALSPDGQHLFATNTPDNRLEIFRIEGHRLVHTGSV
                     PVGLEPVAVAARSEREVWVVNHLSDSVSVVALDGDADSGRVVRTLLVGDEPRDVVFAG
                     PHRQRAFITTAHRGQNIPFAPKLTTAGVGRADVWVFDAGALGDSLGGTPLTIVTLFTD
                     TPRALAVTPDGSRVYAAGFHTGNQTTAVSDQVILDGFGPDGVVGPATNVEGKPAPEVA
                     EIVQWNGSHWVNTAGKSRDDFVKFSLPDKDVFVLDAMASPPRQLSGPAGFFQHVGTIL
                     YSMVVNPVTGKVYVANTDANNAERFEGPGIFAGHSLRGHLHDSRITVLSSGGGVAPRH
                     LNKHIDFHHCCAPVPNPENAKSLALPQGMAVTRNGATLYVAALGSDKVGVFSTAQLED
                     DSFVPSTASQ"
BASE COUNT          389 a          895 c          712 g          381 t
ORIGIN      
        1 ctccggctcg cccagagccg cgtcgccgag gccgtggccg cagccgagga tgccgtcgcg
       61 cgctgcacga ccatgggcgg ctgcggcatg ttccgcggcg cgttcgtccg gctcgcccgc
      121 gccgaggcgc tgcacgccag cggtgcgcat gacgccgctc gccatgccat cgcggacgcg
      181 caaggccggt tgctcgcgat cgcagaccgg atcggcgacc ctgcttacca gcacagcttc
      241 ttgcacaacg tgccagaaaa tgcgaggacc ctcaccctgg tgcgaagctg gctgggtgag
      301 accgcttctg gcacataact tcacccgctg aaaacttctg caggcacggg tatcgccggg
      361 tacctcgccg tcacggcgca ggcggcgccg gtgatcgcgc atgcgcacag gaccgaccca
      421 caccaaggaa tgcaactctc atacatcggt catccgatgt ggctggagcg atgcagcatg
      481 cacgacggca gtggatccac cactgcgcgt ccatgcggcg atgctgctgt gatctacaca
      541 acggcaatgc tgccgcagat cgtgtcaccg caccacgatc acctcgagtc gatgcgtcga
      601 tgcgtcgatg cgtcgatgcg tcacttcgtg tcatcccgcc acgatcatca caaatccgca
      661 acggcagcgt gcgtcgtcac cgggtgacca tcacaaattc acgaccgggg atcgcgtcat
      721 cgtgtcacgc tcggtctggg tacgtggttg ccattcgatc tacgtaacag ccatgagtgc
      781 ttgacggttc gagcacaact ctgttgccaa gtgttggcgt tttcgttctt gacaaataga
      841 ttacgaactt acaaggtatc tttgagtttc tcgacccacg actaagtcgt gcagtcctgc
      901 tgggtcggca gcgcatataa caactgtcca acggggggaa tagcgatgga ttcgcgacac
      961 gcatccggcg gcccgacgag gccgtccact gccgtcatgg ctgtctgtgt cctggggctc
     1021 tctgcggcgc cgtcgtgcgc gccaccggtc gacgctgacc gcgccgccgg tgtcgacgag
     1081 cccggccgcg ccgacgccgt ggtcgatcgc cagactgccg ccacccggcc agcgtctgcg
     1141 agctcgtcga acacgtcctc gttcgcgctg ttcgagagcg gacaggtccg cccgctcgcg
     1201 ctgtcgcccg atggccagca cctgttcgcg accaacacgc cggacaaccg cctggagata
     1261 ttccggatcg aaggccatcg cctggtccac accgggtcgg tcccggtcgg cctcgagccg
     1321 gtcgccgtcg ccgcgcgctc cgagcgcgag gtctgggtcg tcaaccacct gtccgacagc
     1381 gtcagcgtgg tcgcgctcga cggcgacgcc gactccggcc gcgtcgtccg caccctgctg
     1441 gtcggcgacg agccccgcga cgtcgtgttc gccgggccgc atcgccagcg cgccttcatc
     1501 accaccgccc accgcggcca gaacatcccg ttcgctccca agctcacgac cgccggcgtc
     1561 ggccgtgccg acgtctgggt attcgacgcc ggcgcgctcg gcgactcgct cggcggcacc
     1621 ccgctcacca tcgtcaccct gttcaccgac accccgcgcg ccctcgcggt cacccccgac
     1681 ggctcccggg tctacgccgc cggcttccac accggcaacc agaccacggc ggtgagcgat
     1741 caggtgatcc tcgacggttt cgggccggac ggcgtcgtcg gcccggccac caacgtcgag
     1801 ggcaagccgg cgcccgaggt cgccgagatc gtccagtgga acggcagcca ctgggtcaac
     1861 accgccggca agtcacgcga cgacttcgtc aagttctcgc tgcccgacaa ggacgtgttc
     1921 gtgctcgacg cgatggccag cccgccgcgc cagctgtccg gccccgccgg cttcttccag
     1981 cacgtcggca ccatcctgta ctccatggtc gtcaatccgg tcaccggcaa ggtctacgtc
     2041 gccaacaccg acgccaacaa cgccgagcgg ttcgaaggcc ccggcatctt cgcgggccac
     2101 agcctgcgcg gccacctcca cgacagccgg atcaccgtgc tgtcctccgg cgggggcgtc
     2161 gctccccgcc acctcaacaa gcacatcgac ttccaccact gctgcgcacc ggtccccaac
     2221 cccgagaacg caaagtccct cgccctgccc cagggcatgg ccgtcacccg caacggcgcc
     2281 acgctctacg tcgccgccct gggctccgac aaggtcggcg tgttctccac cgcccagctc
     2341 gaggacgaca gcttcgtgcc cagcaccgcc agccaga
//
LOCUS       DASVOR010001181         5117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_12727,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001181 DASVOR010000000
VERSION     DASVOR010001181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5117
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12727"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VF516_22430"
     CDS             <1..535
                     /locus_tag="VF516_22430"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEX8110514.1"
                     /translation="AEALAAMPAGERQARAQATVAVMRGTGYAFAYSSYPLVDAYLGK
                     WAPGGPHDVLLEYINGAPVLDLVRAITGVPELVKADAQATLYAPHHFLAVHNDANSEA
                     GRRVAYVLNLCGEDWRPDWGGYLNFYDDDGDVIAGYRPRFNTLNLFAVPQRHNVSFVP
                     PFAPMARYAITGWFRDR"
     gene            765..2012
                     /gene="frc"
                     /locus_tag="VF516_22435"
     CDS             765..2012
                     /gene="frc"
                     /locus_tag="VF516_22435"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF003809.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HEX8110515.1"
                     /translation="MSKALEGVRVLDMTHVQSGPTATQILAFFGADVIKIEKPGEGDP
                     TRGQLRDVPGADSLYFTMLNGNKRSLTLNPKTEAGKRIFTRLIETCDIMVENFGPGAI
                     DRMGFPWKKIQEINPRMIYASVKGFGPGPYADCKVYENVAQCTGGAASTTGEIDSVPL
                     VTGAQIGDSGTGIHLVAGVLAALYQREKTGRGQRVECAMQDAVLNLCRVKLRDQQRLK
                     HGPLKEYPQYPNGKFGTAVPRAGNASGGGHPGWVVKCKGWETDPDAYIYVITQAQAFP
                     ALAKACGHADWLEDPNFNTPEARLPRLDHVFGEIEKWTMTLTKFEVMEVLNPLNVPCG
                     PVLSMKELAEEPALRETGTVVEVDHPTRGKYLTVGNPIKLSDSPAEVRRSPLLGEHTD
                     EILRKVLGFSDAEVEAARKQGAV"
     gene            2015..3079
                     /locus_tag="VF516_22440"
     CDS             2015..3079
                     /locus_tag="VF516_22440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110516.1"
                     /translation="MATRVLILGASYGSLFGTKLLMAGQTVALVCTRSTAELIGRDGT
                     VVKFTVKGRDGLLDVPSRKLPGQLSASAPEDVDPAAFDLVVLGMQEAQYGAPGVRELM
                     ARVARARTPCLAIMNMPPLPYLRRIPGLDTRAVEGCYTDPGVWEGFDPDLVTLASPDP
                     QAFRPPDQPKNVLQVGLPTNFKAARFAGDAPTQLLRQLEAGIEEARFDPGTGPIEIPV
                     KLKVHDSLFVPLAKWPMLITGNYRCILADRMISIQEAVHGNLGRSREIYDWVAKLCTG
                     LGADPEDLVPFDKYAKAAESLGKPSSAARALFGGAAHIERVDCLVRRIAAQRGMTLDT
                     LDETVALVDQRLTRNRASAS"
     gene            complement(3109..4536)
                     /gene="oxlT"
                     /locus_tag="VF516_22445"
     CDS             complement(3109..4536)
                     /gene="oxlT"
                     /locus_tag="VF516_22445"
                     /inference="COORDINATES: protein motif:HMM:TIGR04259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate/formate MFS antiporter"
                     /protein_id="HEX8110517.1"
                     /translation="MTKNEESASSTTNEGSSSSTKSKEVAASVDVEVARARLKSGRWW
                     QLVFGVICMAMIANLQYSWTLFVTPIHDKYHWDTALIQTAFTIFVLFETWLVPVEGWL
                     VDRFGPRAVVVTGGVVCAIGWGLSAFADSLPFLYVAYGIAGVGAGAVYGTCVGNALKW
                     FPERRGLAAGITAAGFGAGAAATVLPLQWAIKSFGYEATFLWCGLIQGAVILVFGMLL
                     AQPDANAVAALPKPVTMLTNRPQFKWHQMLRTPVFWVMYLMFVLMGAGGLFVTANLKQ
                     IAAAFKVADVPVAFLGLAPTAITFALFLDKIMNGLTRPFFGWISDRIGRENTMFIAFG
                     FEAIGILLLSQLGTSPAAFVVLTGMVFFAWGEIYSLFPSTCADTYGWKYAATNSGALY
                     TAKGTAALFVPLAVVLAKWASWQSVFMVAAAMNGLAAVMALAVLKPLRQRMMAKSSSR
                     AESEAEDSVAAAVAEPLQQMAAQPK"
     gene            complement(4977..>5117)
                     /locus_tag="VF516_22450"
     CDS             complement(4977..>5117)
                     /locus_tag="VF516_22450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_273701379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HEX8110518.1"
                     /translation="AAQARALGFDAARVFVPHPIQDRTDDEMRALADAAVDEVVARLL
                     AR"
BASE COUNT          855 a         1769 c         1741 g          752 t
ORIGIN      
        1 cgccgaggcg ctggcggcga tgccggccgg cgagcgccag gcccgcgcac aggcgacggt
       61 cgcggtgatg cggggcaccg gctacgcgtt cgcgtactcg agctacccgc tggtcgacgc
      121 ctacctcggc aagtgggccc ccggcggccc gcacgacgtc ttgctcgagt acatcaacgg
      181 cgcgccggtc ctggacctgg tccgcgcgat caccggcgtc cccgagctgg tcaaggcaga
      241 cgcccaggcg acactctacg cgccgcacca cttcctggcg gtccacaacg acgcgaacag
      301 cgaggccggc cgccgggtcg cctacgtgct caacctgtgc ggcgaggact ggcggcccga
      361 ctggggcggc tacctcaact tctacgacga cgacggcgac gtgatcgccg gctaccggcc
      421 gcggttcaac acgctcaacc tgttcgccgt gccgcagcgc cacaacgtca gcttcgtccc
      481 gccgttcgcc cccatggcgc gctacgcgat cacggggtgg ttccgcgatc gctgactctg
      541 tttggaactc cgacaggtct gatgtcgcct gtttggcgac gcgcggcgga ttttcgacgc
      601 ctcggctcgt cggccgggtg cccgcccgtc gcccgggtga aaacaccgtt ctggctcacg
      661 gatgcgccac ggcggcgcgg cgtcgggtac agtcgcaaag cgcgtccgga gcctcgcggc
      721 ccgacgaacc gcacgagcaa cgaacccagt aaggagcgca aaagatgagt aaggctctcg
      781 aaggcgtgcg cgtcctggac atgactcacg tgcagtccgg acccacggcc acgcagattc
      841 tggctttctt cggagcggac gtcatcaaga tcgagaagcc gggcgagggg gatccgacgc
      901 gcgggcagct gcgcgatgtc ccgggcgccg acagcctgta cttcacgatg ctgaacggca
      961 acaagcgcag cctgaccctc aaccccaaga ccgaggcggg caagcggatc ttcacccgcc
     1021 tgatcgagac gtgcgacatc atggtcgaga acttcgggcc gggcgcgatc gaccgcatgg
     1081 gcttcccgtg gaagaagatc caggagatca acccgcggat gatctacgcg tcggtcaagg
     1141 gcttcggccc cggcccgtat gcggattgca aggtgtacga gaacgtcgcg cagtgcaccg
     1201 gcggcgcggc gtcgaccacc ggcgagatcg acagcgttcc cctggtgacc ggcgcccaga
     1261 tcggcgactc cggcaccggc atccacctcg tggccggcgt gctcgccgcg ctctaccagc
     1321 gcgagaagac cggtcgcggc cagcgcgtcg agtgcgcgat gcaggacgcc gtgctcaacc
     1381 tgtgccgggt caagctgcgc gaccagcagc ggctcaagca cggtccgctc aaggagtatc
     1441 cgcagtaccc caacggcaag ttcgggaccg cggtgccgcg tgccgggaac gcctcgggcg
     1501 gcggccaccc gggctgggtc gtgaagtgca agggctggga gaccgatccc gacgcgtaca
     1561 tctacgtgat cacacaggcc caggcgttcc ctgcgctcgc caaggcgtgc ggtcacgccg
     1621 actggctcga ggacccgaac ttcaacacgc ccgaggcccg gctgccccgg ctcgaccacg
     1681 tgttcggcga gatcgagaag tggaccatga ccctgaccaa gttcgaggtc atggaggtgc
     1741 tcaacccgct caacgtgccg tgcggcccgg tgctgtcgat gaaggagctc gccgaggagc
     1801 cggcgctgcg cgagaccggc accgtcgtcg aggtcgacca cccgacgcgc ggcaagtacc
     1861 tgacggtcgg aaacccgatc aagctgtcgg attcgcccgc cgaggtccgg cgctcgccgc
     1921 tgctcggcga gcacaccgac gagatcctgc gcaaggtgct cgggttcagc gacgccgagg
     1981 tcgaggccgc gcgcaagcaa ggtgcggtct agccatggcg acccgtgtcc tgatcctcgg
     2041 agcgtcctac ggctcgctgt tcggcaccaa gctgctgatg gccgggcaga ccgtcgcgct
     2101 ggtctgcacc cggtcgaccg ccgagctgat cggccgcgac ggcacggtcg tgaagttcac
     2161 ggtcaagggc cgggacgggc tgctcgacgt gccgtcgcgc aagctgccgg gccagctgtc
     2221 ggcgagcgcg cccgaggacg tcgatcccgc cgcgttcgat ctcgtggtgc tcggcatgca
     2281 ggaggcgcag tacggggcgc ccggcgtccg cgagctgatg gcccgcgtcg cccgggcgcg
     2341 cacgccgtgc ctggcgatca tgaacatgcc gccgctgccc tatctgcggc ggatccctgg
     2401 cctcgacacc cgggcggtcg agggctgtta caccgatccc ggggtatggg agggcttcga
     2461 tccggacctg gtgacgctcg ccagccccga tccgcaggcg ttccgtcccc cggatcagcc
     2521 caagaacgtg ctgcaggtcg gcctgccgac caacttcaag gcggcgcggt tcgccggcga
     2581 tgccccgacc cagctgctgc gccagctgga ggccgggatc gaggaggcgc ggttcgaccc
     2641 gggtaccggg ccgatcgaga ttccggtcaa gctcaaggtc cacgactcgc tgttcgtgcc
     2701 gctcgccaag tggccgatgc tgatcaccgg caactaccgc tgtatcctgg ccgaccgcat
     2761 gatctcgatc caggaggccg tgcacggcaa cctcggccgc tcgcgcgaga tctacgactg
     2821 ggtcgcgaag ctgtgcaccg ggctcggcgc cgacccggag gacctggtcc cgttcgacaa
     2881 gtacgccaag gccgccgagt cgctcggcaa gccgtcgtcg gccgcgcgcg cgctgttcgg
     2941 cggcgccgcg cacatcgagc gcgtcgactg cctggtccgg cggatcgccg cccagcgcgg
     3001 gatgacgctc gacacgctcg acgagaccgt cgcgctcgtc gatcagcgcc tcacgcgcaa
     3061 ccgcgcgtcg gcgtcctgag tcagggcgcc aaggccggcg ctcgcggatc acttcggctg
     3121 cgcggccatc tgctgcagcg gctcggccac ggccgccgcc acgctgtcct cggcctcgct
     3181 ctccgcgcgc gaggagctct tcgccatcat gcgctggcgc agcggcttca gcacggcgag
     3241 cgccatgacc gccgccaggc cgttcatcgc agcggcgacc atgaatacgg actgccagct
     3301 cgcccacttc gccaggacga ccgcaagagg cacgaacagc gccgcggtgc ccttggcggt
     3361 gtacagcgcg cccgagttgg tggcggcgta cttccaaccg taggtgtctg cgcaggtcga
     3421 ggggaacagg ctgtagatct cgccccaggc gaagaacacc atgccggtca gcaccacgaa
     3481 cgccgccgga ctggtgccga gctggctgag cagcaggatg ccgatcgcct cgaacccgaa
     3541 cgcgatgaac atcgtgttct cgcggccgat ccggtcggat atccagccga agaacggccg
     3601 cgtgagcccg ttcatgatct tgtcgaggaa cagcgcgaac gtgatcgccg tgggcgccag
     3661 cccaaggaag gcgaccggca cgtcggccac cttgaaggcc gcggcgatct gcttgaggtt
     3721 cgcggtcacg aacagcccgc cggcgcccat caggacgaac atgaggtaca tgacccagaa
     3781 caccggcgtc cggagcatct ggtgccactt gaactgcggc cggttggtga gcatggtcac
     3841 tggcttgggc agcgccgcga cggcgttggc gtcgggctgg gcgagcagca tgccgaagac
     3901 caggatcacc gcgccctgga tgagcccgca ccacaggaag gtcgcctcgt agccgaacga
     3961 cttgatcgcc cactgcagcg gcaggacggt ggccgccgcg ccggcgccga agccggcggc
     4021 ggtgataccg gccgccagcc cgcgccgctc ggggaaccac ttgagcgcgt tgccgacgca
     4081 ggtgccgtac accgcgccgg cgccgacgcc ggcgatcccg taggcgacgt agaggaacgg
     4141 cagcgagtca gcgaacgcgc tcaatcccca gccgatggcg cagaccacgc cgccggtgac
     4201 caccaccgcg cgcggaccga accgatcgac cagccagccc tcgaccggga ccagccaggt
     4261 ctcgaagagg acgaagatgg tgaacgccgt ctgaatcagc gccgtgtccc agtggtactt
     4321 gtcatggatc ggcgtgacga acagcgtcca gctgtactgg aggttcgcga tcatggccat
     4381 gcagatgacg ccgaacacca gctgccacca gcgcccggac ttcagccggg cacgcgccac
     4441 ttcgacgtcg accgaggcag ccacctcctt gctcttcgtg gacgagctgg atccctcgtt
     4501 cgtggtcgac gaagcggatt cctcgttctt ggtcatgggc gtcctcctag gcgaggtcga
     4561 ggtctttatg tccgaccgct atgtgtattt ttccagtaac gacttcagtg gaattgctag
     4621 gggcggaata ccacggtccc ggcacatctt ggaagaccgt gaatcaggtc gtctcgtatc
     4681 gggcgcagct cccgccaggt tggagggtcg aagcacacac atttgccgac gagtgccagc
     4741 ccggacggag cacaacacaa gtccgaccgt tcggtccaca tcggttcacc atcgatcgct
     4801 tcacgtgatg cattcgcggg acggcagatt ttcgtggggt atgtgtgggg tatgcggtgt
     4861 gagggggacg gaggtcgcgg cgcaccgagt caggacccgt gtggccgcgt acgagccgcc
     4921 ttcagggtag tcggatttcc ggctttgcaa atggccaatc gactcgagcg cgcggcctac
     4981 cgcgcgagca ggcgcgccac gacctcgtcg accgcggcgt cggccagcgc gcgcatctcg
     5041 tcgtcggtgc ggtcctggat cgggtggggc acgaacacac gcgctgcgtc gaagccgagc
     5101 gcacgggcct gcgccgc
//
LOCUS       DASVOR010001182         4011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_245862,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001182 DASVOR010000000
VERSION     DASVOR010001182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245862"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF516_22455"
     CDS             complement(<1..204)
                     /locus_tag="VF516_22455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110519.1"
                     /translation="MDDRARALWRRHIKLDVIATAPLRGDAAAARAELIRRGEHASAD
                     VGRRAASRWPSSRRRVKRVALGAR"
     gene            1090..1878
                     /locus_tag="VF516_22460"
     CDS             1090..1878
                     /locus_tag="VF516_22460"
                     /inference="COORDINATES: protein motif:HMM:NF024053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8110520.1"
                     /translation="MAGAIAGGVIAIPAALKGFVEILLLADAGDITGEGANLFKTFGR
                     EVAGVSAAAVREWLAGEARQFCEREVQAAAERFASDPAWIALSDDEQRRVMEIAWYEQ
                     MRRFFRGFGKAAKDAEPELKDAAKVAMEAAGVEPVAGRLPANHEYAGKEFPREQLPRK
                     YRENGLRVKSTGYPDFEPYAFTLPKGKKTVRIELTGSHRADVALANKAAGFNKTLPPP
                     PKYTWHHLEDEGEMMLVPTDLHVSVPHTGGKAHFKHRTGITYAN"
     gene            1889..2368
                     /locus_tag="VF516_22465"
     CDS             1889..2368
                     /locus_tag="VF516_22465"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX8110521.1"
                     /translation="MERRGHEIAIANVLRLEQEIGVRLPDDYRQFLLEVNGGRTTDAH
                     REFMMRKGEQIGQVTLDLLHSLDDQSDDGFELREQQLFRRYDYPENGLRIGYASGWAL
                     VLILSGPHRGELWILAIQDPPIEADQRIEWFDRPDAWKVADSFAEFMAGLRPSDAGA"
     gene            complement(3093..>4011)
                     /locus_tag="VF516_22470"
     CDS             complement(3093..>4011)
                     /locus_tag="VF516_22470"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX8110522.1"
                     /translation="RETEMSDSTSRQPERSSGTAAASVPDRLWAVPIYLPYLQPALTD
                     RGVEQAEAKLGVRLPRAYLAALRVQNGGYLRLEVHPSGHVDYVAGIGPRFPSLLQHSW
                     AGTMEYMRSQEIETPVSIDRLIPFCGDGHWFYCLDYRQTGCEGEPYITYVDVECFDVD
                     RVVAPDFATFLSELKPDPEDLRYGLVTHDGPTDVAAAISNVTGFTFEDLGDQHNGYRQ
                     FRATLPGEHQSAWLTANRTRRGFVREDDPEYKALREQFTELVDRYPEDSDCGYFLSCS
                     DFDSRAGQAIARSLTKLPFAARALPADTR"
BASE COUNT          701 a         1292 c         1420 g          598 t
ORIGIN      
        1 ccgagcgccg agcgcgaccc gcttgacgcg ccggcgactg ctgggccagc ggctggcagc
       61 acgacggccg acgtcggccg atgcgtgctc gccgcgccga atgagctcgg cgcgcgccgc
      121 cgcggcatct ccgcgcagcg gagcagtcgc gatgacatcc agcttgatgt gacgtcgcca
      181 tagcgcccgg gcgcggtcgt ccactgccgc gagccgcgct cgctcgccgc cgacggccgc
      241 gacctgtccg ggccttgttg aagaggagcg ccggcgacgg tagtgccatc ggcgctcagt
      301 cgcggctcgc taacgtgagg cgcgctggtg accgctcgga ccgtcggtca ttcctccgag
      361 accatgggcg gcgggttcga tagacaaccg agcacgggcg cgcagcatgg cacggctagc
      421 gctagcgcgg cgcccgacct cggtcctggc aagcggacgc tgaccgagca gctcggccct
      481 ggagccgcac catcgcgagc tgtggccgag catgtcgagc ccaacctcaa cgcgctgatc
      541 gccgtcgtcg cgcgtggtgc ggacggcgcc gaactcgtgc gctggcgagc tcgtgggcac
      601 tgggatgggc cgctgccggt gcggtaccac ggctcgcgtg caccgatggg ctggacatgg
      661 agcgacaagg ctgcgcgaga cacgcggatc cacacgctgg ctgatgggac cggcggtgag
      721 tcgatcgagc gatgggcgac ggcacatggt gcggcaacgg ttgacgtgat cgcgttcggc
      781 atcggagcga caccgcagcc cgacaccgcg gacgatcccg atccggtcgg caggctcgag
      841 cgcgtgttcg ggcgcaggac cggcgctggc gctcctggcg gagacccgca tggccgaacc
      901 gggcttgaca cagcgatcgg cggcaccgga ccgggcggac cccacgcgcg atcaggtggc
      961 gaccacgagg gctccaagac gcgcgggcta acccaggaca gcaggttcgg atcggcgctc
     1021 ggtgtcaacg ggggcgccct cggtggccgg ttcggtggcg agggcaagcc cggagacaac
     1081 ggcgtgcgca tggcgggtgc gatcgccggc ggcgtgatcg cgatccctgc ggcgctcaag
     1141 ggcttcgtcg agatcctgct cctggccgac gcgggcgata taaccggcga gggcgccaac
     1201 ctgttcaaga cgttcggccg cgaggtggcc ggcgtcagcg cggcggcggt ccgcgagtgg
     1261 ctcgcgggcg aggcgcgcca gttctgtgag cgcgaggtcc aggcggcggc ggagcggttc
     1321 gcgagcgatc cagcgtggat cgcgctatcc gacgacgagc agaggcgcgt gatggagatc
     1381 gcgtggtacg agcagatgcg ccggttcttc cgcggcttcg gcaaggcggc gaaggatgcc
     1441 gagccagagc tcaaggatgc ggcgaaggtc gcaatggagg ccgccggggt cgagccggtc
     1501 gccggtaggc tgccggccaa ccatgaatac gcaggcaagg agttcccgcg cgagcagttg
     1561 ccgcggaagt accgggagaa tggactgcgg gtcaagtcca cggggtaccc ggacttcgag
     1621 ccgtacgcgt tcacgctgcc gaagggcaag aaaacggtgc ggatcgagct gaccggatcg
     1681 caccgagcag acgtcgcgct ggcaaacaag gcggccgggt tcaataagac gctgcctccg
     1741 cctccgaaat acacctggca ccatctcgaa gacgagggcg agatgatgct ggtgccgacg
     1801 gatctccatg tgagcgttcc ccacaccggc gggaaggcac acttcaaaca cagaacaggg
     1861 attacctatg ccaactagct ggccgccaat ggagcgccgt ggacacgaga tcgcgatcgc
     1921 caacgtccta cgcctcgagc aggagatcgg agtccgactt cccgacgact atcggcagtt
     1981 cctgcttgag gtcaacggag gccgcaccac ggacgcgcac cgcgaattca tgatgcggaa
     2041 aggcgaacag atcggccaag tcacgctaga cttgctgcac agcctggacg atcagagcga
     2101 cgatggtttc gagctcaggg agcagcaact gtttcggcga tacgactacc cagagaacgg
     2161 cttgcgcatc ggctacgcca gcggatgggc gctcgtcttg atcctgtcgg gtccgcatcg
     2221 cggcgagctc tggatactcg cgatccaaga cccgccgatt gaggccgacc agcgcatcga
     2281 atggttcgat cggcctgacg cgtggaaggt cgccgacagc ttcgccgagt tcatggccgg
     2341 gttgcgaccg agcgacgctg gagcctgacg gccgacccgg gcgtccgtgc gcggaccgtg
     2401 gactgatcag cgggctgcgc acggaacatc gaacaagggc ggccgcggcg ggccgggacc
     2461 tgtacccgcg acagctcatg atgtggggcg tacgacccag cgcaggcgcc tcgcgttcca
     2521 ggagctcgtt cgcacggaag gtcgcgacgc cgagtaactc ggctcgtcgc gcgagcgcgc
     2581 ggggcgattt cggcacgctg gcgcgggaac atcgccggcg cgatcgccga gttggaggtc
     2641 ctcgccgcgc tgctcgccgc cctggcggcg tcgaactggg gtcaccgcac agccctcccc
     2701 cgccgaccag gtcgcgaccc ggcgctcgac ggcgctgagc tcgatggcca ccaccgccgc
     2761 cagcgcgatc gccgccatgg ccgcgacgcc gacgatcgcg atgccggcga ccaggctgcg
     2821 acccggcgct cgacgaccgc cgagcttcca gcaactcgcc gccgccgcgg atctacgggg
     2881 tccacgtcga gggcggagac ctggtgatcc gcggaatacg gggaactcac gcgctggacg
     2941 tttcgcccga gcggcgatcg cgcgcgtcgg ccaggcggca tggctcagct ttctcggcgg
     3001 tcgtgcaggg gccaagatga cgccgacttt cgagcggccc tgcgcggcgc gctgagccgt
     3061 caggggcagg gccgctcaag catcgtcacg ccctatcgcg tatcggcggg tagcgcacga
     3121 gcagcgaacg gcagcttcgt gaggctgcgg gcaatggctt gacctgctct gctgtcgaag
     3181 tcggagcagc tcaggaaata gccacagtcc gaatcctctg ggtagcgatc gacaagttca
     3241 gtgaattgct cgcggagcgc cttgtattca gggtcgtctt ctcgaacgaa gccgcgccgc
     3301 gtgcggttcg ctgtcagcca cgcggactga tgttcgccgg gaagcgtcgc ccggaactgg
     3361 cgatagccgt tgtgctggtc tccgagatcc tcgaaggtga accctgtgac gttcgagatc
     3421 gcagcggcga cgtcggtcgg cccatcgtga gtcaccaggc cgtaccggag atcctccggg
     3481 tctggcttga gctcgctgag aaacgttgcg aagtcaggtg caacgactct atcaacatcg
     3541 aagcattcga cgtcaacgta ggtgatgtat ggttcaccct cgcatcctgt ctggcgatag
     3601 tcgaggcaat aaaaccagtg tccgtctcca cagaatggga tcaatcgatc aatgcttact
     3661 ggagtctcta tctcctggga ccgcatgtac tccatggtgc cggcccagct gtgctggagc
     3721 aacgatggga atctgggacc gatcccggcg acgtaatcta cgtgaccgct ggggtgaact
     3781 tcgagacgaa ggtacccgcc gttctgcacg cgcagcgcgg cgaggtaagc acgcggcaag
     3841 cgcacgccga gcttcgcctc tgcctgctcg acaccgcgat cggtgagcgc gggttgcaga
     3901 tacgggaggt agatcggaac agcccagagg cgatccggca cactggcagc agcggtcccg
     3961 gaggaccgct ctggctggcg tgacgtacta tcactcatct cggtctccct g
//
LOCUS       DASVOR010001183         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362794,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001183 DASVOR010000000
VERSION     DASVOR010001183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362794"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VF516_22475"
     CDS             <1..485
                     /locus_tag="VF516_22475"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEX8110523.1"
                     /translation="GESICHDGACLTVTDVDPGRGAYRVLAGAETLARTALGALAVGK
                     RVNLERALRVGDRLGGHWVTGHIDGMGELVARRDLGANLVLEFRAAPALLRYVVEKGS
                     IAVAGVSLTVNSVDAGGFSVAIIPHTRDLTSLGDLAVGDRVNLEVDILAKHVEKLVKP
                     "
     gene            482..1687
                     /gene="ribB"
                     /locus_tag="VF516_22480"
     CDS             482..1687
                     /gene="ribB"
                     /locus_tag="VF516_22480"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEX8110524.1"
                     /translation="MTSSVTPGPVPVPSTAIDRVTRALEDIRAGRMVILVDDEDRENE
                     GDLVFAADMVTPEHINFMATHARGLICLAMDDAMIDRLELPMMVRDNRASLGTAFTVS
                     IEARHGVSTGISARDRATTIRTAIADNASPAHIVSPGHVFPLRARKGGVLVRTGQTEG
                     SVDLARLAGCRPAGVICEIMRDDGEMARRPELEQFAARHGLFLLSVADLIAYRLQRER
                     IVRRQGTGALRPGALGPGEPFVAHYYDTEVEDTEYLALVRGDVAAVSATGQPVLVRVA
                     AADPLADPFVLRPDLIAAVEAIDQAGVGVLLYVVNRSRTRLGPSFERLVLQRSAPRDL
                     ARARDDSSEALRDFGLGAQVLADLGLHRIRLMMWSDRKIAGLEGFGIEIVERVPVPGR
                     AGARRREAQ"
     gene            1719..2192
                     /gene="ribH"
                     /locus_tag="VF516_22485"
     CDS             1719..2192
                     /gene="ribH"
                     /locus_tag="VF516_22485"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEX8110525.1"
                     /translation="MPQLIEGHLSAAGLRFAIVASRFNALIVEPLIAGALDAIVRSGG
                     DADAVTIVRCPGAWELPQVVRKVVLRGGIDAVIALGAVIRGATPHVDSVAGEAAHGIA
                     GVAAASDVAVSFGVLTTDTIEQALERAGTKAGNKGFDAALAAIELATLFRRLGAR"
BASE COUNT          277 a          829 c          796 g          304 t
ORIGIN      
        1 ctggcgagtc gatctgccac gacggcgcgt gcctcacggt caccgacgtt gaccccggcc
       61 gcggcgcgta ccgcgtgctc gccggcgccg agacgctcgc ccgcaccgcg ctcggcgcgc
      121 tcgccgtggg caagcgggtc aacctcgagc gcgcgctgcg cgtcggcgac cggctgggcg
      181 gccactgggt caccggccac atcgacggca tgggcgagct ggtcgcgcgc cgcgatctcg
      241 gcgccaacct cgtgctcgag ttccgcgcgg cgccggcgct cctgcgctac gtcgtcgaga
      301 agggctcgat cgccgtcgcc ggcgtgagcc tgacggtcaa ctccgtcgac gccggcgggt
      361 tctcggtcgc gatcatcccg cacacccgcg acctgaccag cctcggcgac ctcgccgtgg
      421 gcgatcgggt caacctcgag gtcgacatcc tcgccaagca tgtcgagaag ctggtgaagc
      481 catgacgtct tccgtcacgc ccggccccgt gcccgtcccg tcgaccgcga tcgaccgcgt
      541 cacccgcgcc ctggaggaca tccgcgccgg ccggatggtg atcctggtcg acgacgagga
      601 ccgcgagaac gagggcgacc tggtgttcgc ggccgacatg gtcacgcccg agcacatcaa
      661 cttcatggcg acccacgcgc gcggcctgat ctgcctggcg atggacgacg cgatgatcga
      721 tcggctcgag ctgccgatga tggtgcgcga caaccgggcg tcgctcggca ccgcgttcac
      781 cgtgtcgatc gaggcccggc acggcgtgtc gaccgggatc tcggcgcgcg atcgcgccac
      841 gacgatccgc accgcgatcg ccgacaacgc cagcccggcg cacatcgtgt cgcccggcca
      901 cgtgttcccg ctgcgcgccc gcaagggcgg cgtcctggtg cgaaccggcc agaccgaggg
      961 ctcggtcgac ctcgcgcggc tcgccggctg ccgcccggcc ggcgtgatct gcgagatcat
     1021 gcgcgacgac ggcgagatgg cgcggcgccc cgagctcgag cagttcgccg cgcgccacgg
     1081 cctgttcttg ctcagcgtcg ccgacctgat cgcctaccgc ctgcagcgcg agcggatcgt
     1141 gcggcgccag ggcaccggcg cgctgcggcc gggcgcgctc gggcccggcg agccgttcgt
     1201 cgcgcactac tacgacaccg aggtcgagga caccgagtac ctcgcgctgg tgcgcggcga
     1261 cgtcgcggcg gtgtcggcca ccggccagcc ggtgctggtc cgggtcgccg cggccgatcc
     1321 gctcgccgat ccgttcgtgc tgcggcccga cctgatcgcc gcggtcgagg cgatcgacca
     1381 ggccggcgtc ggtgtcctgc tctacgtcgt caaccgcagc cggacccggc tcggcccgtc
     1441 gttcgagcgg ctcgtgctgc agcggagcgc cccgcgcgac ctcgcccggg cccgcgacga
     1501 ctccagcgag gcgctgcgcg acttcggtct gggcgctcag gtgctcgccg atctcgggct
     1561 ccaccggatc cggctcatga tgtggagcga tcggaagatc gccgggctcg aaggtttcgg
     1621 catcgagatc gtcgagcgcg tgcccgtccc ggggcgcgcc ggcgcacggc ggcgcgaggc
     1681 ccagtagccg cggagccgat ggtgtatatc tgcggggcat gccgcagctg atcgaaggcc
     1741 acctgtccgc cgcgggcctc cggttcgcga tcgtcgcgag ccggttcaac gcgctgatcg
     1801 tcgagccgct gatcgccggg gccctcgatg ccatcgtgcg atcgggcggc gacgccgacg
     1861 cggtgaccat cgtccgctgc ccgggcgcgt gggagctgcc gcaggtcgtg cgcaaggtcg
     1921 tcctgcgcgg tggcatcgac gccgtgatcg ccctcggcgc ggtgatccgc ggcgcgacgc
     1981 cgcacgtcga ctccgtcgcc ggcgaggccg cgcacggcat cgccggggtc gcggcggcca
     2041 gcgacgtcgc ggtcagcttc ggcgtgctga ccaccgacac catcgagcag gccctggagc
     2101 gcgccggcac caaggccggc aacaaggggt tcgacgctgc gctggcggcg atcgagctcg
     2161 ccacgctgtt ccgccggctc ggtgcgcgat gaccaccggc acccgg
//
LOCUS       DASVOR010001184         2836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_567620,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001184 DASVOR010000000
VERSION     DASVOR010001184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567620"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VF516_22490"
     CDS             complement(<1..368)
                     /locus_tag="VF516_22490"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEX8110526.1"
                     /translation="MIRRTRVEVDLAAIIGNARTVRAVTGTDVIAVVKADGYGHGAVA
                     VARALTGARAVAGFGVSLVEEGVALRDAGVMAPVLVMGPSQHGGEDDMVMAGLTPVIA
                     SAEELAALAAVARRRGRTIDA"
     gene            complement(508..1119)
                     /locus_tag="VF516_22495"
     CDS             complement(508..1119)
                     /locus_tag="VF516_22495"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8110527.1"
                     /translation="MTVRKVPVGSAVVAQDEPGDAMFVIMSGRVKVVIFGESGREVTL
                     SLLRSGDSFGEMSLFDQAPRSAHCLAIEPTALLVLSREDLLRHMQSHPRTAMNLLGEM
                     ARRLRRADETIAQLALCDVNERLIHRLVGLAREEGASGPDGLVVRRRPTQQELANMIG
                     SCRETISRAFNQLARDGLIIPRGRSLVVTPALIERAEKKSKAA"
     gene            1554..1766
                     /gene="thiS"
                     /locus_tag="VF516_22500"
     CDS             1554..1766
                     /gene="thiS"
                     /locus_tag="VF516_22500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEX8110528.1"
                     /translation="MSVTIDVVINGQPRTVNQGTSIAGLIGELGLSGRPVAVERNREV
                     VPRALHASTLLAAGDRLEVVTFVGGG"
     gene            1769..2551
                     /locus_tag="VF516_22505"
     CDS             1769..2551
                     /locus_tag="VF516_22505"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEX8110529.1"
                     /translation="MPEPSTSDTWTLGARTFRSRIIVGTGKYASNEQTRDALAASGAE
                     IVTVALRRIDLSSKQTVLDAIDRSRYVLLPNTAGCYTADDAIRTLHLARELGMDELVK
                     LEVIGDPKTLFPDNTATVEAAQRLVKEGFQVLPYCSDDLVTCKRLQDAGCLAVMPLAA
                     PIGSGLGIRNPHNIRLILENVSVPVIVDAGVGTASDAAIAMELGCTAVLMNTGIAGAR
                     QPVLMAEAMRLAVDAGRKAFLAGRMPRRDYANASSPTTQLIE"
     gene            2650..>2836
                     /locus_tag="VF516_22510"
     CDS             2650..>2836
                     /locus_tag="VF516_22510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110530.1"
                     /translation="MVAPDLLAAGAWPAIAAAFGAAVERAVAGCPAGSVVVQVREKDL
                     DGGPLLALVRAARPFAPI"
BASE COUNT          414 a         1135 c          871 g          416 t
ORIGIN      
        1 gcatcgatcg tacgcccgcg ccgccgcgcg accgccgcca gcgctgcgag ctcctcggcg
       61 ctggcgatca ccggcgtcag ccccgccatc accatgtcat cctcgccgcc gtgctggctc
      121 ggtcccatca ccagcacggg agccatgaca ccggcgtcgc ggagcgcgac accttcctcg
      181 accaggctca ccccgaagcc ggccaccgcc cgcgccccgg tcagcgcgcg cgccaccgcc
      241 acggcgccgt gcccgtagcc gtcggccttg accaccgcga tcacgtcggt cccggtcacc
      301 gcgcggaccg tccgcgcgtt gccgatgatc gcagccaggt cgacttcgac gcgggtgcgc
      361 cggatcatcg tcgacgacgg catacccggg ggatcacccg gatcctccgt catgtcaagc
      421 cgaccacgcg ctccggcgcg atccatgcaa tcgcgcacca ccccgcgcac caccccgtcg
      481 cgcaccaccc cggccggctg cagcgggcta cgccgccttg gacttcttct cggcgcgctc
      541 gatcagcgcc ggcgtcacca ccagcgaccg gccgcgcggg atgatcagcc cgtcgcgcgc
      601 cagctggttg aacgcccgcg agatcgtctc gcgacagctg ccgatcatgt tcgccagctc
      661 ctgctgggtc ggccggcggc gcaccaccag cccgtcgggt cccgacgcgc cttcctcgcg
      721 cgccagcccc accagccggt ggatcagccg ctcgttgacg tcgcacagcg ccagctgcgc
      781 gatcgtctca tcggcgcggc gcagccggcg cgccatctcg ccgagcaggt tcatcgcggt
      841 ccgcgggtgg gactgcatgt ggcgcagcag atcctcgcgc gacagcacca gcagcgcggt
      901 cggctcgatc gccaggcagt gcgccgagcg cggcgcctgg tcgaacagcg acatctcccc
      961 gaacgagtcc cccgagcgca gcagcgagag cgtcacctca cggccgctct caccgaagat
     1021 caccaccttc acccgccccg acatgatcac gaacatcgca tcccccggct catcctgcgc
     1081 caccaccgcc gaccccaccg gcaccttccg gaccgtcaca cgcgacgtca gatcttcgat
     1141 cgcgctgatc ggcagcgccg caaacagcgg tgctctcgca atcgtggctc ggatccgggc
     1201 cggatcttct cccccgttgc gtgggtctac aggcctcagt gtgggcatcg aagcggggga
     1261 catgacaggg cgggcaattc ctagggtcat agtttcctcg ggggggtgac aacctaccgt
     1321 gtgctcccct ctacaaagct catgcccgtg acccggatct cggcccggga acgctgtttc
     1381 agtaatttga cgcctatcct acaccccggg agtgtgattg ttacttcaca cccctgtggg
     1441 aaaccgaggc gctgatgtcg tggtccttac acctagcggg gtgcgtcacg gcgaacggag
     1501 gagttctggc ctggggcgag ccaccccggg gcggtggtgg taggttcgcc gcgatgtcgg
     1561 tgacgatcga cgtggtcatc aacggccagc cgcggacagt gaaccaggga acctccatcg
     1621 ccggtctcat cggtgagctc ggtctgtccg gtcgcccggt cgcggtcgaa cgcaaccgcg
     1681 aggtcgtccc gcgcgccctg cacgcgtcga ccctgctcgc tgcaggcgac cgcctggagg
     1741 tcgtcacctt cgtgggagga ggctgaacat gccagagccc agcaccagcg acacctggac
     1801 cctcggcgcg cggaccttcc gctcgcgaat catcgtcggg accggcaagt acgcctccaa
     1861 cgagcagacc cgcgacgccc tcgccgcctc gggcgccgag atcgtcaccg tcgccctgcg
     1921 ccgcatcgac ctgtcgagca agcagaccgt gctcgacgcc atcgaccgca gccgctacgt
     1981 gctcttgccc aacaccgccg gctgctacac cgccgacgac gcgatccgca ccctccacct
     2041 cgcccgcgag ctcggcatgg acgagctggt caagctcgag gtcatcggcg atcccaagac
     2101 cctgttcccc gacaacaccg ccaccgtcga ggccgcccag cgcctggtca aggagggctt
     2161 ccaggtcctg ccgtactgct ccgacgacct ggtcacctgc aagcggcttc aggacgccgg
     2221 ttgcctcgcc gtcatgccgc tggccgcccc gatcggctcg ggcctgggca tccgcaaccc
     2281 ccacaacatc cgcctgatcc tcgagaacgt ctccgtcccg gtcatcgtcg acgcgggcgt
     2341 cggcaccgcg tccgacgccg cgatcgccat ggagctcggc tgcaccgccg tcctcatgaa
     2401 caccggcatc gccggcgccc gtcagcccgt cctcatggcc gaggccatga ggctcgcggt
     2461 cgacgccggc cgtaaggcct tcctcgccgg ccgcatgccc cgccgcgact acgccaacgc
     2521 ctcgtccccg accacccagc tgatcgagtg atggccccca acctgtcggg gactgttgac
     2581 cccggcgacc cgccggccgg ccgatctctt caggccgtgc gtgtcatcgc ggttacggat
     2641 cgccgccgca tggttgcgcc cgacttgctc gccgccggcg cctggcccgc catcgccgcc
     2701 gcgttcggcg ccgcggtcga gcgcgccgtc gccggctgcc ccgccggcag cgtcgtcgtc
     2761 caggtccgcg agaaggacct cgacggcggc ccgctcctgg cgctggtccg cgccgcccgg
     2821 ccgttcgccc cgatcc
//
LOCUS       DASVOR010001185         2301 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_391474,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001185 DASVOR010000000
VERSION     DASVOR010001185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391474"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..876)
                     /locus_tag="VF516_22515"
     CDS             complement(<1..876)
                     /locus_tag="VF516_22515"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8110531.1"
                     /translation="MRASRLVSILMMLQTRGRVSARVLADHLEVSVRTVYRDIDELSA
                     AGVPVVVERGAAGGFELLDGWRTTLTGLTPDESQTLFLSGLPGPAAELGLGDTMASAH
                     LKLLAALPPEWQREAERVSSRFHVDLAGWYRRIAPVRHLRALADAVWADRWIAIRYES
                     WQGIVDRELEPLGLVIKAGEWYLAAQPHGGGRGRAPSPRTYRVSNLRELTVHGAFTRP
                     ARFDLFRLLERVDPAVRASAAVEDAIDAAIAQPPGVGADGWLRVTIPIKSIGHATGQM
                     LSLGTDAEVLGPPELR"
     gene            1044..2219
                     /locus_tag="VF516_22520"
     CDS             1044..2219
                     /locus_tag="VF516_22520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8110532.1"
                     /translation="MQRRDFLKTSMVGVSAAASAVGLAQAMAAPGGPGAGRGAPPAPR
                     GSGAPHPGQADAQSLDPHRLLRVLDPGERRGDMLVRTLGHTGEKVSLIGMGGAHLSRP
                     PLDERGATRLIHAALDRGINFLDNCWDYGHGNSERWMGVALAQGGYRQKAFLMTKLDG
                     RTREAAKSQLDESLSRLKTDHIDLVQFHEILRFDDPDRIFADGGALEALVAAKQAGKV
                     RYIGFTGHKDPRIHLYMLEVARRKGFLFDSVQMPINAMDAHFRSFSHLVVPELVSHDI
                     GILAMKTMGDGVLLKTGAPLSAIDYLHYAMNLPVSVVITGIDSDKILDQAFEATRTFK
                     PMSADQVAALVARTRPYATDGKYEFFKTTSHFDSTAKHPDWLGEDPPVVQRLAPSVG"
BASE COUNT          350 a          786 c          870 g          295 t
ORIGIN      
        1 gcgcagctcg ggcgggccga ggacctcggc gtcggtgccg agcgagagca tctggccggt
       61 ggcgtggccg atggacttga tcgggatggt gacgcgcagc cagccatcgg cgccgacgcc
      121 gggtggctgg gcgatcgcag catcgatggc gtcctcgacc gccgcgctgg ctcgaaccgc
      181 cgggtcgact cgctccagta ggcggaagag atcgaagcgg gcggggcggg tgaacgcgcc
      241 gtgcacggtg agctcgcgga ggttcgagac gcggtaggtc cgcggcgacg gggcgcggcc
      301 gcggccgccg ccgtgtggct gggccgcgag gtaccactcg ccggccttga tcaccaggcc
      361 gagcggctcg agctcgcggt cgacgatgcc ctgccagctc tcgtagcgga tcgcgatcca
      421 gcgatcggcc cagacggcgt cggcgagggc gcgcaggtgg cggaccggcg cgatccggcg
      481 gtaccagccg gcgaggtcga cgtggaaccg cgagctgacg cgctcggcct cgcgctgcca
      541 ctcggggggc agggcggcga gcagcttgag gtgggcggag gccatggtgt cgccgagccc
      601 gagctcggcg gccgggccgg gcaggccgga caggaacagg gtctgcgact cgtcgggggt
      661 caggccggtc agggtggtgc gccagccgtc gagcagctcg aacccgccgg cggcgccgcg
      721 ctcgacgacg accggcacgc ccgccgcgct gagctcgtcg atgtcgcggt acacggtgcg
      781 gacggagacc tcgaggtggt cggcgagcac gcgcgcgctg acccggccgc gggtctgcaa
      841 catcatgagg atcgacacca gccggctcgc gcgcatggat ctcgacgatg gcgatcatac
      901 atgacacgac ctggcaagca tggcggcgca ggctgggccc atgggcgccg aagctcaccc
      961 caggccgaag ccggcgccga ggatgcgccg cagccggccg ccgcccgggc gcggcgctac
     1021 gatcgatcat gacgcaacga tccttgcagc gacgcgattt cctcaagacg agcatggtcg
     1081 gggtcagtgc ggcggcgagc gcggtcgggc tggcccaggc gatggcggcc ccgggcgggc
     1141 cgggcgctgg ccggggtgca ccaccggcac cgcgcgggtc gggtgctcca catccgggtc
     1201 aggccgacgc gcagtcgctc gatccgcacc ggctgctgcg cgtcctcgac cccggcgagc
     1261 ggcgcggcga catgctggtt cgcacgctcg ggcacaccgg cgagaaggtg tcgctgatcg
     1321 gcatgggcgg cgcgcacctc agccgcccgc cgctcgacga gcgcggcgcg acccggctga
     1381 tccacgccgc gctcgaccgc gggatcaact tcctcgacaa ctgctgggac tacggccacg
     1441 gcaacagcga gcgctggatg ggcgtggcgc tggcccaggg cgggtatcgc cagaaggcgt
     1501 tcctgatgac caagctcgac ggccggaccc gcgaagcggc gaagtcgcag ctcgacgagt
     1561 cgctgagccg gctcaagacc gaccacatcg atctcgtgca gttccacgag atcctgcggt
     1621 tcgacgatcc ggatcggatc ttcgccgacg gcggcgcgct ggaggcgctg gtcgccgcca
     1681 agcaggcggg caaggtgcgc tacatcggct tcaccgggca caaggacccg cggatccacc
     1741 tctacatgct ggaggtcgcg cggcggaagg ggttcctgtt cgacagcgtg cagatgccga
     1801 tcaacgcgat ggatgcgcac ttccgcagct tctcgcacct ggtcgtcccc gagctggtga
     1861 gccacgacat cggcatcctc gcgatgaaga cgatgggcga tggcgtgctg ctcaagaccg
     1921 gggcgccgct gtccgcgatc gactacctgc actacgcgat gaacctgccg gtgtcggtcg
     1981 tgatcaccgg gatcgacagc gacaagatcc tcgatcaggc gttcgaggcg acgcggacgt
     2041 tcaagccgat gtccgccgat caggtcgcgg cgctggtcgc caggacccgg ccgtacgcga
     2101 ccgacggcaa gtacgagttc ttcaagacga cgagccactt cgactcgacc gccaagcacc
     2161 cggactggct cggcgaggat ccgccggtgg tccagcggct cgccccgtcg gtcggctgac
     2221 gccgcgcggt cacggcgtga actcggcgcc gcgctcgagc agccgcagcg ggatcaccgc
     2281 gtcggccgcg ggcgccggat c
//
LOCUS       DASVOR010001186         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_538101,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001186 DASVOR010000000
VERSION     DASVOR010001186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538101"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="VF516_22525"
     CDS             <1..239
                     /locus_tag="VF516_22525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110533.1"
                     /translation="REILVQGAVSFTKHCLEELSKDGKTTVDATNVIRGGAYAEAEWE
                     NGAWRHRASTQRMAVVIEFHTETKLIAVTSFVFR"
     gene            312..812
                     /locus_tag="VF516_22530"
     CDS             312..812
                     /locus_tag="VF516_22530"
                     /inference="COORDINATES: protein motif:HMM:NF027067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II TA system antitoxin MqsA family protein"
                     /protein_id="HEX8110534.1"
                     /translation="MKCPQCRNEMIRKTENHRYTESGLPNVVLVGVEVEHCKNCGENL
                     VSIPNIEGLHRAIAMALIRHPGRLAPNEIRFLRKWLGWSGVDFAKHMGVTPETVSRWE
                     SVENGKAMGGTAERLLRLAVAHGQPIDEYPIDMLAEVSDDQVPKSALLAMKPVRSGWV
                     PTSLAA"
     gene            complement(898..>2119)
                     /locus_tag="VF516_22535"
     CDS             complement(898..>2119)
                     /locus_tag="VF516_22535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110535.1"
                     /translation="PPMPQPPSPGNASTLSAGQVTRGARPSSSRRMLWIALAAIVLTG
                     GGITAGALLASRGSGSTDPGAGNAGNAGNAGNGSPPAGAPVTATTGAPGKAQSDPWAT
                     PAQQAPPKAQPPPPASDSKDDSKDDDDDDDAPNSAPGANPADDAIAAALKQLDATIKS
                     LPPAQQKRLAAYKGLSKLPPAERLKRLRQLTQQAMAEAPDPAAIARAAGAAVDDDAPA
                     GPSAPAKWVTNQSIDPPGGYDPEHLDVSAFVPWAIAQARKAIPDAQMFRIDADGVGPD
                     GRANLKLPTLASAHGSIDVRFISPSRGKRDPKQPLGVARHDFKCEFRVMATPDGVELM
                     PIDFFDCAKEHVVPVPRCTLTAVWKKAIARKAPAHNAVSNVGYRSSGSRPVWYFDIGF
                     GTDVSFSEMFGDDC"
BASE COUNT          320 a          674 c          757 g          368 t
ORIGIN      
        1 ctcgcgagat cctcgtccag ggggcggtca gcttcacgaa gcactgcctg gaggagctca
       61 gcaaggatgg gaagacgacc gtcgatgcaa cgaacgtcat tcgtggcggc gcttacgccg
      121 aggctgaatg ggaaaatggc gcctggcgcc atcgggcctc gacgcaacga atggctgtgg
      181 tgatcgagtt ccatacggag accaagctga tcgccgtgac ctcgttcgtg ttcaggtagc
      241 ggaccgattg ctgccaaggc ggccaacagc tccaaggcac cttctgtgcc tccagggagg
      301 agcacaagat catgaagtgt ccacagtgcc gcaacgagat gattcgcaag accgagaatc
      361 atcgctacac cgaatcaggc ttgccgaatg tcgtactcgt cggcgtcgag gtagagcact
      421 gcaaaaattg tggcgagaat ttggtttcga tcccgaatat cgaagggctg cacagggcca
      481 ttgcgatggc gctgatccga catcctggtc gcctcgcgcc gaacgagatc cggttcctgc
      541 ggaaatggct tggctggtct ggcgtcgatt tcgcgaagca catgggcgtg acaccggaaa
      601 cggtgtctcg ctgggaaagc gtagagaatg gcaaggcgat gggcggtaca gcggagcgtc
      661 tgctccgttt ggccgttgct catggtcagc ccatcgatga gtatccgatc gacatgctgg
      721 ctgaagtcag cgatgaccag gtgccgaagt ctgcgctcct cgccatgaag cccgtgcgta
      781 gcggctgggt tccgacaagt ctcgcagcgt aactcggcgc gcgcatggga ctcggtgcgg
      841 cgcgagcgag aacttcgcca gatgatcgca gctcatgttg cgaggttccg gtgatcgcta
      901 gcagtcgtcg ccgaacatct cgctgaacga cacgtcggtg ccgaagccga tgtcgaagta
      961 ccacaccggc cgcgagccac tggatcgata gccgacgttg ctgaccgcgt tgtgcgccgg
     1021 cgccttgcgg gcgatcgcct tcttccacac ggcggtcagg gtgcaccgcg ggaccggcac
     1081 gacgtgctcc ttggcgcagt cgaagaagtc gatcggcatc agctcgacgc cgtcgggagt
     1141 cgccatgacc cggaactcgc acttgaagtc gtggcgggcg acgccgagcg gctgcttcgg
     1201 atcgcgcttg ccgcgcgatg gcgagatgaa ccggacgtcg atgctgccgt gcgcggaggc
     1261 cagcgtcggc agcttgaggt tggcgcggcc gtcggggccg accccgtcgg cgtcgatccg
     1321 gaacatctgg gcatcgggga tcgcctttcg ggcctgcgcg atcgcccacg ggacgaacgc
     1381 cgagacgtcg aggtgctcgg ggtcgtagcc gcccggcgga tcgatgctct ggttggtgac
     1441 ccacttcgcc ggggcgctcg ggcccgccgg ggcgtcgtcg tcgaccgccg cgccggccgc
     1501 ccgggcgatc gcggccggat cgggggcttc ggccatcgcc tgctgggtga gctggcgcag
     1561 ccgcttcagc cgctcggcgg gcggcagctt ggacagcccc ttgtacgcgg cgagccgctt
     1621 ctgctgggcg ggcggcagcg acttgatcgt ggcatcgagc tgcttcagcg ctgcggcgat
     1681 cgcgtcgtcc gcggggttcg cgcccggtgc gctgttcggt gcgtcgtcgt cgtcgtcatc
     1741 gtccttgctg tcgtccttgc tgtcgctggc cggcggcggt ggctgtgcct tcggcggcgc
     1801 ctgctgcgcc ggagtcgccc acggatcgct ctgcgccttg cccggcgcgc cggtggtcgc
     1861 agtgaccggg gcgccggccg gcgggcttcc attcccggca ttcccggcat tcccggcatt
     1921 cccggcgccc gggtcggtcg agccgctgcc ccggctggcc agcagggcgc ccgcggtgat
     1981 gccgccgccg gtcagcacga tggcggccag cgcgatccac agcatcctcc tcgaggacga
     2041 cggccgggcg ccccgggtga cctggccggc cgacagcgtc gaggcgttgc ccggcgatgg
     2101 cggctgcggc atgggcggc
//
LOCUS       DASVOR010001187         1921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362820,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001187 DASVOR010000000
VERSION     DASVOR010001187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362820"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..991)
                     /locus_tag="VF516_22540"
     CDS             complement(173..991)
                     /locus_tag="VF516_22540"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEX8110536.1"
                     /translation="MTANAYTPTMLAIDRAVREIDGVVTFTLTPPGGFRFAPGQFNML
                     YVHGVGEVAISISGDPAEPAQLVHTVRSVGTVTRPMTALGVGSVLGVRGPYGSAWPVD
                     AVRGRDVLVIAGGLGLAPLRPAILHLLANRSSYGRITILYGARSPAELLFREELQRWR
                     GRFDCRVEVIVDHAGHDWYGAIGVVTQLLDGVAVDRDDAALVCGPEIMMRFVARALEQ
                     RGVARDGIWISMERSMKCGVGLCGHCQLAGSFVCKDGPVFRYDRLAPLMQVREL"
     gene            complement(988..1437)
                     /locus_tag="VF516_22545"
     CDS             complement(988..1437)
                     /locus_tag="VF516_22545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX8110537.1"
                     /translation="MEDLERVLKSHPFLADLAPEHLSFMVSCAKNARFRPGEYLMREG
                     DREDQLYLIRHGTVTIEMPGPSGAPICLETIGSGDVLGVSSLTDAAAHLDCRARDTVV
                     AIALDNACLHRKMDEDPRLGYAISMRLLERTYERLTRARLQRLDVYR"
     gene            complement(1440..>1921)
                     /locus_tag="VF516_22550"
     CDS             complement(1440..>1921)
                     /locus_tag="VF516_22550"
                     /inference="COORDINATES: protein motif:HMM:NF028488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8110538.1"
                     /translation="TADDLAAAAVVTDAAAARMGRTMPAAGLRDALMANLEHPRWNDV
                     AARCLGCANCTMVCPTCFCTTVEDTTDLSGEVATRARRWDSCFTAEYAYVHGGSVRPT
                     LRARYRQWLTHKLATWHDQFGTSGCVGCGRCIAWCPVGIDITEEAAAICAPGSATRS"
BASE COUNT          280 a          740 c          649 g          252 t
ORIGIN      
        1 ccctcgacca gcgacaggtc gaacggcccg ggcgcggtgt gcgacgtcgc ctcggggaag
       61 tacgcgatgt cgaccgcgcc ggcgaccgcg agcagctcgt cctcgcagtc gagcaggctg
      121 agctggcagc cgtcgcacga cgcgaacttc cacaccgcga ggctcgggcg ggtcatagct
      181 ccctcacttg catcagcggg gccagccggt cgtagcggaa caccggcccg tccttgcaca
      241 cgaacgatcc ggcgagctgg cagtgcccgc acagcccgac gccgcacttc atgctgcgct
      301 ccatcgagat ccagatcccg tcgcgcgcca cgccgcgctg ctccagcgcg cgggcgacga
      361 accgcatcat gatctccggc ccgcagacca gcgccgcgtc gtcgcgatcg accgccacgc
      421 cgtcgagcag ctgggtcacc acgccgatcg cgccgtacca gtcgtggccg gcgtggtcga
      481 cgatcacctc gacccggcag tcgaaccggc cgcgccagcg ctgcagctcc tcgcgaaaca
      541 agagctccgc cggcgagcgc gcgccgtaca ggatcgtgat ccggccgtac gacgagcggt
      601 tggccagcag gtgcaggatc gccggccgca gcggcgccag cccgagcccg ccggcgatca
      661 ccagcacgtc gcggccgcgc accgcgtcga ccggccacgc cgagccgtac ggcccgcgga
      721 ccccgagcac gctgcccacg ccgagcgcgg tcatcggccg cgtcaccgtg ccgaccgagc
      781 gcacggtgtg caccagctgc gcgggctcgg ccggatcgcc gctgatcgag atcgcgacct
      841 cgccgacgcc gtgcacgtac agcatgttga actggcccgg cgcgaaccgg aacccgccgg
      901 gcggcgtcag cgtgaacgtc accacgccgt cgatctcgcg caccgcgcgg tcgatcgcca
      961 gcatcgtcgg ggtgtaggcg ttggccgtca tcggtacacg tcgagccgct gcagccgggc
     1021 gcgggtcagc cgctcgtagg tccgctcgag cagccgcatc gagatcgcgt agcccagccg
     1081 cggatcctcg tccatcttgc ggtgcaggca cgcgttgtcg agcgcgatcg ccaccaccgt
     1141 gtcgcgtgcc cggcagtcga ggtgggcggc cgcgtcggtc agcgacgaca ccccgagcac
     1201 gtcgcccgat ccgatggtct ccaggcagat cggagcgccg ctcggacccg gcatctcgat
     1261 cgtcaccgtg ccgtgccgga tcaggtacag ctggtcctcg cgatcgccct cgcgcatcag
     1321 gtactcgccg ggccggaacc gcgcgttctt cgcgcagctg accatgaacg acaggtgctc
     1381 cggcgcgaga tcggccagga atggatgcga cttgaggacg cgctcgaggt cttccatcgt
     1441 cagctccttg tcgcggatcc cggggcgcag atcgcggccg cttcctcggt gatgtcgatc
     1501 ccgaccgggc accacgcgat gcaccggccg cacccgacgc accccgaggt cccgaactga
     1561 tcgtgccagg tcgccagctt gtgggtcagc cactgccggt agcgcgcccg cagcgtcggc
     1621 cgcacgctgc cgccgtggac gtacgcgtac tcggcggtga agcacgagtc ccaccgccgc
     1681 gcgcgggtcg cgacctcgcc cgacaggtcg gtggtgtcct cgacggtggt gcagaagcac
     1741 gtcgggcaca ccatcgtgca gttcgcgcag cccaggcagc gcgccgcgac gtcgttccac
     1801 cgcgggtgct cgaggttcgc catcagcgcg tcgcgcagcc cggccgccgg catcgtccgc
     1861 cccatccgcg cggccgccgc gtcggtcacc accgccgccg ccgcgaggtc gtccgccgtc
     1921 g
//
LOCUS       DASVOR010001188         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362822,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001188 DASVOR010000000
VERSION     DASVOR010001188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362822"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /locus_tag="VF516_22555"
     CDS             complement(<1..843)
                     /locus_tag="VF516_22555"
                     /inference="COORDINATES: protein motif:HMM:NF041244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein IglI family
                     protein"
                     /protein_id="HEX8110539.1"
                     /translation="MDSSTVRLNLDRLGAGLYRDGEPASPGLELGDPRLDAINDTAFK
                     GNYAEAAALALSVWDEGIRDLRLIGYLLYGYYLERDVLALPFIFEQLTQTLSTRWDRI
                     GPARKDKPADGALHWLFASLIRQLDGHEKARDERYQGWLSPEGTAAFAAAESASAALL
                     VEAEKRFAQGKCFHKLRNLSGRLRDIAAALRIPTVEDKPAAAEDNPPSAAADKHAAET
                     AAAAPAEDRRAGVVTVEGGAALGLLLRKLRLFERLVQHNQPRKAAVVARDVEQLLGAF
                     DPIAF"
     gene            complement(848..1201)
                     /locus_tag="VF516_22560"
     CDS             complement(848..1201)
                     /locus_tag="VF516_22560"
                     /inference="COORDINATES: protein motif:HMM:NF025472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4280 domain-containing protein"
                     /protein_id="HEX8110540.1"
                     /translation="MPSHIDHGELAHCQYGTAPAPVHASGHIPVATARDFVPFENIHP
                     FGACLCPANPQVAAATAAAMGVLTPMPCVPAVSEEWQVGNSHVWVQGVRALDGQSSVR
                     CRWGGLIQLVNPGKR"
     gene            complement(1201..>1642)
                     /locus_tag="VF516_22565"
     CDS             complement(1201..>1642)
                     /locus_tag="VF516_22565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110541.1"
                     /translation="EHFLYGGTRGPRSAGRVYPIAEVVAVARKHPRVIDAAVVLDRSG
                     RFGQPVRILCAFTGAARPQPGDEDALARAIGQELGAELVPDHVQLLPLYARLRGGEPD
                     PRWCLSQQVTGQFYRKPRQEPTQALTLLRRAIAAGADPDTTRSR"
BASE COUNT          233 a          547 c          632 g          230 t
ORIGIN      
        1 gaaggcgatc ggatcgaagg cgccgagcaa ctgctcgacg tcacgggcaa ccaccgccgc
       61 cttgcgcggc tggttgtgct gcaccagccg ctcgaacagg cgcagcttgc gcagcaagag
      121 gccgagcgcg gcgccgccct cgaccgtgac gacgccggcg cgcctgtcct cggcgggcgc
      181 cgcggcggca gtctcggcgg cgtgcttgtc cgcagcggcg gaagggggat tgtcctcggc
      241 ggcagcgggc ttgtcctcga cggtggggat gcgcagcgca gcggcgatat cgcggagcct
      301 gccgctcagg ttgcgcagct tgtggaagca cttgccctgg gcgaagcgct tctcggcctc
      361 gaccagcagt gcggcgctgg ccgactcggc agcggcgaac gcggcggtgc cctcggggct
      421 gagccagccc tggtagcgct cgtcgcgcgc cttctcgtgc ccgtcgagct gccggatcag
      481 cgatgcgaac aaccagtgga gcgccccgtc ggcgggcttg tctttcctgg ccgggccgat
      541 gcggtcccag cgcgtgctca gcgtctgggt gagctgctcg aagatgaacg gcagcgccag
      601 cacgtcgcgc tcgaggtagt aaccgtagag caggtagccg atgaggcgca ggtcgcggat
      661 gccctcgtcc cacacgctca gcgccagcgc ggcagcctcg gcgtagttgc ccttgaacgc
      721 agtgtcgttg atggcgtcga ggcgggggtc gccgagctcg agaccgggcg aggccggctc
      781 gccgtcgcga tagaggccgg cgccgaggcg atcgagattg aggcgaacgg tcgaggagtc
      841 catgggctta tcgctttcct gggttgacga gttggatgag gccgccccag cggcagcgca
      901 ccgaggactg cccgtcgagc gcgcgcacgc cttgcaccca tacatgcgag ttgccgacct
      961 gccactcctc ggagacggca ggcacgcacg gcatcggtgt caagacgccc atggcggcgg
     1021 cggtggcggc ggcgacctgc ggattggcag ggcacaggca ggcgccgaac ggatggatgt
     1081 tctcgaacgg cacgaagtcg cgtgcggtgg cgacggggat gtggccgctg gcgtggacgg
     1141 gcgcgggtgc ggtgccgtac tggcagtgcg cgagctcgcc gtggtcgata tggctcggca
     1201 tcaccggctc ctcgtggtat cggggtcagc gccagcggcg atcgcgcggc gcagcagcgt
     1261 cagcgcttga gtcggctcct ggcgcggctt gcgatagaac tggccggtca cctgctgcga
     1321 caggcaccag cgcggatcgg gctcgccgcc gcggaggcgc gcgtagagcg gcagcagctg
     1381 cacgtgatcg gggaccagct cggcgccgag ctcctggccg atggcgcgcg ccagcgcgtc
     1441 ctcgtcgccg ggctgcggcc gcgcggcgcc ggtgaacgcg cacaggatgc gcaccggctg
     1501 gccgaaccgg cccgagcgat cgagcaccac cgcggcgtcg atcacgcgcg gatgcttgcg
     1561 cgccaccgcc accacctcgg cgatcgggta cacgcgaccg gccgagcgcg ggccgcgcgt
     1621 accgccgtag aggaagtgtt cg
//
LOCUS       DASVOR010001189         3681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_100279,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001189 DASVOR010000000
VERSION     DASVOR010001189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100279"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF516_22570"
     CDS             complement(<1..256)
                     /locus_tag="VF516_22570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110542.1"
                     /translation="MRYLALATYLAAGLWGLDLTMLAGCGAAPPRPIVRAGPGVTPAR
                     DLATARTEAPREPHDERKLSRDPRVIDLDIIRITARAKGPG"
     gene            364..2775
                     /locus_tag="VF516_22575"
     CDS             364..2775
                     /locus_tag="VF516_22575"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8110543.1"
                     /translation="MKVAFTYNLRLTDVRETEKEAEYDSAETVSAIAAALEAAGHEVE
                     KVEVSGPASNLLERLEQIDPDIIFNTAEGQGGRMREAFYPALFDELGIPYTGSDAYTN
                     AVTLDKWLTKLIVSRANIDTARGQLVTVRNFDQVIERGAGLAFPVIVKPNHEGSSKGI
                     YNGALGSSVVKDPKDLPGVLKSALRQYPDGVLVEEYIAGLDIAVGFIEGVGHDDGLLT
                     PVEVMYDPSPGISERGFNIYDYRLKNVEPGKVQYRCPANLPRDVAARLRSISSEAIRV
                     LGLRDLARLDYRVTAEGRIYLLEANALPALASSSSLFAATAQVGLTYTATIASVLNAA
                     ALRSGLATASQLGVGRQRKAQPIRVGFTYNVKRSHDGDDEAEWDPPETIISIANALAR
                     QGHIVVHLEATPDLPRVLAEADVDLIFNIAEGVEGRNREAQVPALCELLGIPYTGSDS
                     ATLAIALDKALGKKVLLQHDILTPKFQVMESARERLSPDMKFPLIVKPNAEGSSKGIA
                     STSVCDTEDELRAAVKSIIERYRQPALVEEYIAGREFTVGLLGDKRPRVLPPMEIKFK
                     KATKRPVYDYAVKQEWEEHVYYECPAKLTEAEQKAIEKIARATFWALDCRDVARVDMR
                     MDADGRIYVLEVNPLPGLTPGYSDLVLIAQAVGMEYDQLIAEIMVGGLRRMKDKRREE
                     RELEREREAQSKDPAKLADKDKAEKKRPVAKLSPAAAASAMASGNGSNGNGGNGNGNG
                     GNGNGGHGSNGNGNGNGGNGNEAAPVRVKRERSEPVTQVATVERARTEPGPPESIDAP
                     PATAT"
     gene            3220..3480
                     /locus_tag="VF516_22580"
     CDS             3220..3480
                     /locus_tag="VF516_22580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110544.1"
                     /translation="MALDIDDVDLDSSVELDGSEAIPRRLTPPSTVDLAPLSRSVEAR
                     HTGSVDVQRRWWAQRRTPPSTSTLWVNDQVDVDDPERHVKDL"
     gene            complement(3499..>3681)
                     /locus_tag="VF516_22585"
     CDS             complement(3499..>3681)
                     /locus_tag="VF516_22585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110545.1"
                     /translation="QVKPTHYKVICISMYTRDIEELEAKVAELKRRGWTKANKSQLIR
                     IALAQVDLDKLATPQQ"
BASE COUNT          631 a         1250 c         1276 g          524 t
ORIGIN      
        1 cgcccgggcc cttcgcgcgc gccgtgatcc ggatgatgtc gaggtcgatc acgcgcgggt
       61 cgcgcgacag cttgcgttca tcgtgcggct cgcgaggcgc ctcggtccgc gcggtcgcga
      121 gatcgcgcgc cggtgtcacg ccggggcctg cgcgcacgat cggccgcggt ggcgcggcgc
      181 cacagcccgc gagcatggtg aggtcgagcc cccacagacc ggcggcgaga tacgtggcca
      241 gcgcgaggta ccgcatgccc aggtatgacg tatcaagcgc gcaaccggtg cgtcgcgatc
      301 tagtcacggg ttgcccttga ctgtccggct gtcacaccgc tctgctaatc ctggcagcaa
      361 cagatgaagg tcgcgttcac gtacaacctc cggctgaccg acgtccgcga gaccgagaag
      421 gaagccgagt acgacagtgc agagacggtg agcgcgatcg cggcagcgct cgaggccgcc
      481 ggccacgagg tcgagaaggt cgaggtctcg gggccggcgt cgaacctgct cgagcgcctc
      541 gagcagatcg atccggacat catcttcaac accgccgagg gccagggcgg gcggatgcgc
      601 gaggcgttct acccggcgct gttcgacgag ctcggcatcc cgtacaccgg cagcgacgcg
      661 tacaccaacg cggtcacgct cgacaagtgg ctcaccaagc tgatcgtgtc gcgcgcgaac
      721 atcgacaccg cgcgcggcca gctggtcacg gtgcgcaact tcgaccaggt gatcgagcgc
      781 ggcgcgggcc tggcgttccc ggtgatcgtc aagcccaacc acgagggctc gagcaagggc
      841 atctacaacg gcgcgctcgg gtcgtccgtg gtcaaggacc ccaaggacct gcccggggtg
      901 ctcaagagcg cgctgcgcca gtaccccgac ggcgtgctgg tcgaggagta catcgccggg
      961 ctcgacatcg cggtcggctt catcgagggg gtcggccacg acgacggcct gctcaccccg
     1021 gtcgaggtca tgtacgatcc gtcgcccggg atcagcgagc gcggcttcaa catctacgac
     1081 taccggctca agaacgtcga gccgggcaag gtccagtacc gctgccccgc caacctgccg
     1141 cgcgacgtcg cggcgcggct gcgcagcatc tcgagcgagg cgatccgcgt gctcgggctg
     1201 cgcgacctgg cgcggctcga ctaccgggtc accgcggagg gccggatcta cctgctcgag
     1261 gccaacgcgc tgccggcgct ggcgtcgagc tcgtcgctgt tcgcggcgac cgcgcaggtc
     1321 ggcctgacct acaccgcgac gatcgcctcg gtgctcaacg cggccgcgct gcgctcgggg
     1381 ctggcgaccg cgagccagct cggggtcggc cgccagcgca aggcgcagcc gatccgcgtc
     1441 gggttcacct acaacgtcaa gcgcagccac gacggcgacg acgaggccga gtgggacccg
     1501 ccggagacga tcatctcgat cgccaacgcg ctggcccgcc agggccacat cgtggtccac
     1561 ctcgaggcca cgccggacct gccgcgggtg ctggccgagg ccgacgtcga cctgatcttc
     1621 aacatcgccg agggcgtcga gggccgcaac cgcgaggcgc aggtgccggc gctgtgcgag
     1681 ctgctgggga tcccgtacac cgggtcggac tcggcgaccc tggcgatcgc gctcgacaag
     1741 gcgctcggca agaaggtgct gctgcagcac gacatcctga ccccgaagtt ccaggtcatg
     1801 gagtcggcgc gcgagcggct gtcgcccgac atgaagttcc cgctgatcgt caagcccaac
     1861 gccgagggct cgagcaaggg catcgcgtcg accagcgtgt gcgacaccga ggacgagctg
     1921 cgcgccgcgg tcaagagcat catcgagcgc taccgccagc ccgcgctggt cgaggagtac
     1981 atcgccggcc gcgagttcac ggttggcctg ctcggcgaca agcggccgcg cgtgctgccg
     2041 ccgatggaga tcaagttcaa gaaggcgacc aagcggccgg tctacgacta cgcggtcaag
     2101 caggagtggg aggagcacgt ctactacgag tgccccgcga agctgaccga ggccgagcag
     2161 aaggcgatcg agaagatcgc gcgcgccacg ttctgggcgc tcgactgccg cgacgtcgcc
     2221 cgggtcgaca tgcggatgga cgccgacggc cggatctacg tgctggaggt caacccgctg
     2281 cccgggctga cccccggcta ctccgacctg gtgctgatcg cccaggcggt cggcatggag
     2341 tacgaccagc tgatcgccga gatcatggtc ggcgggctgc gccggatgaa ggacaagcgg
     2401 cgcgaggagc gcgagctgga gcgcgagcgc gaggcgcaga gcaaggaccc cgccaagctc
     2461 gccgacaagg acaaggccga gaagaagcgc ccggtcgcca agctgtcgcc ggcggcggcc
     2521 gcgtcggcaa tggccagcgg caacggcagc aatggcaacg gcggcaacgg caatggcaac
     2581 ggcggcaacg gcaacggcgg ccatggcagc aacggcaacg gcaacggcaa tggcggcaac
     2641 ggcaacgagg ccgcgccggt gcgggtcaag cgcgagcgat ccgagccggt cacccaggtc
     2701 gccaccgtcg agcgcgcgcg caccgagccc ggaccgcccg aatcgatcga cgcaccccct
     2761 gcgaccgcga cctaggcctg cgcgctgatg attcgtcaca agccttcgat ctttcgcgat
     2821 ccattgacgt cgacctgatc gttgacccag cgcgttgagg tcgacggcga cgtacgacgt
     2881 tgagcccacc atcgacgttg gacgtcgact gatcctgtgc cgcgagcctc gacggagcgc
     2941 gacaacgggg tgggtcgagg tcgacggtgg ggtccgacgt gtacgtatcg cttcgctgcc
     3001 gtcaagctca acgctggggt caaggtcgac gtcatcgtcg acgttcgtcg atgcgcagca
     3061 cggcggcgct cccggtcggc tacactaccg ccggagatga aagtcgcggc ccggatcacc
     3121 gcggcgaccg cggtcgtcgt ggcgctcgcg tcggtgtcgt acgcgctgta gcggcacctc
     3181 tgatgattca tcacaagttg cgcgttcgtg gtggttcccg tggcgctcga catcgacgat
     3241 gtcgaccttg actccagcgt cgagcttgac ggcagcgaag cgataccgag acgtctgacc
     3301 ccaccgtcga ccgtcgacct cgccccgttg tcgcgctccg tcgaggctcg ccacacagga
     3361 tcggtcgacg tccaacgtcg atggtgggct caacgtcgta cgccgccgtc gacctcaacg
     3421 ctctgggtca acgatcaggt cgacgtcgat gatcccgagc gccatgtcaa ggacttgtga
     3481 cgaaccatca gagcggcgct actgctgcgg cgtggccagc ttgtcgagat cgacctgcgc
     3541 cagcgcgatc cggatcagct gcgacttgtt cgccttggtc cagccgcggc gcttgagctc
     3601 ggcgaccttg gcctcgagct cctcgatgtc gcgggtgtac atcgagatgc agatcacctt
     3661 gtagtgcgtc ggcttgacct g
//
LOCUS       DASVOR010001190         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_187579,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001190 DASVOR010000000
VERSION     DASVOR010001190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187579"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VF516_22590"
     CDS             <1..1683
                     /locus_tag="VF516_22590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8110546.1"
                     /translation="IGLSVFAPTPATAGEGFALQAPEIGAGGSWVANTVVSYASNPLI
                     LDSLSADGGRISSAVVSRSTLMQIGLAYALFGRLELGAHLPLYLQSGEAGAAPMAGMA
                     AKPIKSTATGNLAVHLKARILRTAGSSGAFALGASATAALPTATKDQFTGADQPEGRF
                     LVLAAFTPAALDARLTISANAGPVIRKESHFENIVQKSGAAWGGGLSVRILDELWATA
                     ELFGETTPSAERQQPKAGAMPGPAVLSAVEGLAGLTIKPDRRVSIGMAVGRGVTDAIG
                     TPDLRGVVSVSIVPGAAPLAPIHSLVPDGDADGDGIPDSVDKCPSEPEDKDGFQDADG
                     CPDPDNDHDGIPDAQDKCPLDPEDKDGFEDADGCPDPDNDHDGIPDKLDKCPNEPEDK
                     DGFEDLDGCPDPDNDHDGIPDEKDKCPNEPETINGFQDDDGCPDKGDSTIILSPDKIE
                     TLDPIQFTGVKLTRSATPLIEQVGATLRAHPEILRVRVTVHVQPTNDSDADQARSDKR
                     AQAARDWLVQWGVAPARLEARGFGGTKPLVPPDQRGAAKLNERIEFIILERK"
BASE COUNT          285 a          662 c          630 g          200 t
ORIGIN      
        1 atcggactct cggtgttcgc gccgaccccg gccaccgccg gcgagggctt cgcgctccag
       61 gcgccggaga tcggcgccgg cggcagctgg gtcgcgaaca cggtggtgtc gtacgcatcg
      121 aacccgctga tcctcgactc gctgtcggcc gacggcggcc ggatcagcag cgcggtggtg
      181 tcgcgcagca cgctgatgca gatcggcctg gcctacgcgc tgttcggccg cctcgagctc
      241 ggcgcgcatc tgccgctcta cctgcagagc ggcgaggccg gcgcggcgcc gatggcgggg
      301 atggcggcca agccgatcaa gagcaccgcc accggcaacc tggcggtgca cctcaaggcg
      361 cggatcctgc gcaccgcggg gagctccggc gcgttcgcgc tcggcgccag cgcgaccgcc
      421 gcgctgccga ccgcgaccaa ggatcagttc accggcgccg accagcccga gggccgattc
      481 ctggtgctcg cggcgttcac gccggccgcg ctggacgcgc ggctcacgat ctcggccaac
      541 gcgggcccgg tgatccgcaa ggagtcgcac ttcgagaaca tcgtgcagaa gagcggcgcc
      601 gcgtggggcg gcgggctgtc ggtccgcatc ctcgacgagc tgtgggcgac cgcggagctg
      661 ttcggcgaga ccacgccgtc ggccgagcgc cagcaaccca aggccggtgc gatgcctggc
      721 cccgccgtgc tgtcggcggt cgagggtctc gccgggctca cgatcaagcc ggaccgccgg
      781 gtgtcgatcg ggatggcggt cggccgcggc gtgaccgacg cgatcggcac gcccgacctg
      841 cgcggcgtgg tgtcggtgtc gatcgtcccc ggcgccgcgc cgctcgcgcc gatccactcg
      901 ctggtgcccg acggcgacgc cgacggcgac ggcatcccgg acagcgtcga caagtgcccc
      961 agcgagcccg aggacaagga cggcttccag gacgcggacg gctgccccga tcccgacaac
     1021 gatcacgacg gcatccccga cgcccaggac aagtgcccgc tcgaccccga ggacaaggac
     1081 ggcttcgagg acgcggacgg ctgtcccgac cccgacaacg atcacgacgg catccccgac
     1141 aagctggaca agtgccccaa cgagcccgag gacaaggacg ggttcgagga cctcgacggc
     1201 tgccccgacc ccgacaacga tcacgacggc atccccgacg agaaggacaa gtgccccaac
     1261 gagcccgaga cgatcaacgg gttccaggac gacgacggct gccccgacaa gggcgactcg
     1321 acgatcatcc tgtcgcccga caagatcgag acgctcgatc cgatccagtt caccggcgtc
     1381 aagctgacgc gcagcgccac gccgctgatc gagcaggtcg gcgcgacctt gcgcgcccac
     1441 cccgagatcc tccgggtgcg ggtcaccgtg cacgtccagc cgaccaacga ctccgacgcc
     1501 gaccaggcgc gcagcgacaa gcgcgcccag gcggcccgcg actggctggt ccagtggggc
     1561 gtcgccccgg cgcggctcga ggcccgcggg ttcggcggca ccaagccgct ggtgcccccc
     1621 gatcagcgcg gcgcggccaa gctcaacgag cggatcgagt tcatcatcct ggagcggaag
     1681 tagcgcgggc gagcacgagc gcggtgaccg aggccgcccc ggcgcggcgc agcgatcggg
     1741 ccgcggcggc cagcgtggcc ccggtggtca tcacatc
//
LOCUS       DASVOR010001191         1777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_362839,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001191 DASVOR010000000
VERSION     DASVOR010001191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_362839"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VF516_22595"
     CDS             complement(<1..322)
                     /locus_tag="VF516_22595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110547.1"
                     /translation="MAEPAPGRRAARGFVLLNVATIGWGTTFCVIQSAERAGLTAGGV
                     TFLRFLVAALAMAPFVRLDRRVVRAGLELGMWLGTGYIIQAVGLRHTSVGHAAFICTM
                     FVVLV"
     gene            complement(334..639)
                     /locus_tag="VF516_22600"
     CDS             complement(334..639)
                     /locus_tag="VF516_22600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8110548.1"
                     /translation="MSNSPDPAARSADPPVQIDNERVRVTEWRFAPGAATGWHRHELD
                     YVVVPMTTGRLRIDTGGAEAFAELVVGQSYTRVAPVEHNVINANPHELTFVEIELKR"
     gene            complement(636..1598)
                     /locus_tag="VF516_22605"
     CDS             complement(636..1598)
                     /locus_tag="VF516_22605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110549.1"
                     /translation="MPPAEDASPIGPLRARKNLVPLPYWRVSYQRYDGAMTEDALRAH
                     LLGREAYRRTDFVAVHDGAGRHALAAVERRQEPGDPLFTPITAVEVLALPDRCRFVRD
                     RDTDPQCPSALARLARAHAVGPDDTLVVWSMYDHVTFIHRPDPVVIRVVEVAPPDPPK
                     LYQVAQKVLSYADLPPIILELETIDLRALCRSVRASAYLFPCQSGGLEAVGAPVYFLD
                     DRPAERRDWTLIGCHRTLEFHRHFYGDEPPMVSMCPRDLAGPRTRPTLLKCCLQTWKE
                     EHARIASGHLERDGASMVVPWSSDEHLIERALRELATTGPRSEP"
BASE COUNT          244 a          620 c          690 g          223 t
ORIGIN      
        1 gcacgagcac gacgaacatc gtgcagatga acgccgcgtg gccgacgctg gtgtggcgca
       61 gcccgacggc ctggatgatg tagccggtgc cgagccacat gccgagctcg aggccggcgc
      121 gcaccacccg gcggtccagg cgcacgaacg gggccatcgc gagcgccgcg acgaggaagc
      181 gcaggaacgt cacgccgccg gcggtcagcc cggcgcgctc ggcgctctgg atcacgcaga
      241 acgtggtgcc ccagccgatc gtcgccacgt tcagcagcac gaacccgcgc gccgcgcgcc
      301 ggccgggagc tggttccgcc acgccggcct cgctcagcgc ttgagctcga tctcgacgaa
      361 ggtgagctcg tgggggttcg cgttgatcac gttgtgctcg accggcgcga cccgggtgta
      421 cgactgcccg accacgagct cggcgaacgc ctcggcgccg ccggtgtcga tccggagccg
      481 gccggtggtc atcggcacga ccacgtagtc gagctcgtgg cggtgccagc cggtcgccgc
      541 gccgggcgcg aaccgccact cggtgacgcg cacgcgctcg ttgtcgatct ggaccggggg
      601 gtcggcggac cgcgccgccg gatcgggact gttgctcatg gttcactcct ggggccggtg
      661 gtggcgagct cgcgcagcgc gcgctcgatc aggtgctcgt cggagctcca cggcacgacc
      721 atcgacgcgc cgtcgcgctc gaggtggccg ctcgcgatcc gcgcgtgctc ctccttccag
      781 gtctgcaggc agcacttgag cagcgtcggc cgggtgcgcg ggccggcgag gtcgcgcggg
      841 cacatgctga ccatcggcgg ctcgtcgccg tagaagtggc ggtggaactc cagcgtgcgg
      901 tggcagccga tcagcgtcca gtcccgccgc tcggcggggc ggtcgtcgag gaagtacacc
      961 ggcgcgccga ccgcctccag cccgccggac tggcacggaa acaggtacgc gctggcgcgc
     1021 accgagcggc acagcgcgcg caggtcgatg gtctccagct cgaggatgat cggcggcagg
     1081 tcggcgtacg acaggacctt ctgggcgacc tggtagagct tcggcggatc gggcggcgcg
     1141 acctcgacca cgcggatcac caccggatcg gggcgatgga tgaacgtgac gtggtcgtac
     1201 atcgaccaca cgacgagcgt gtcgtcgggg ccgaccgcgt gggcgcgggc gagccgggcg
     1261 agcgcggacg ggcactgcgg atcggtgtcg cgatcgcgca cgaaccggca gcgatcgggc
     1321 agcgcgagca cctcgaccgc ggtgatcggc gtgaacagcg gatcgccggg ctcctggcgg
     1381 cgctcgaccg cggccagcgc gtggcggccg gcgccgtcgt ggacggcgac gaagtcggtg
     1441 cggcggtacg cctcgcgccc gagcaggtgc gcgcgcagcg cgtcctcggt catcgccccg
     1501 tcgtagcgct ggtagctgac ccgccagtac ggcagcggca ccaggttctt gcgcgcgcgc
     1561 aagggcccga tcgggctggc gtcctcggcg ggcggcatcg tgggaccgta gcgcaggcgc
     1621 ccgcggggtt ccagcgcccg ccggcgcgct acggcgcatg gacgaggatg ccgcgcacaa
     1681 gccagctcgc gagggtcggg tcggcgccgg tgccggtgtc ggaaacgagc cgctccagct
     1741 caccgcgcgg gcggggcgtc gccgcggcgg cgagcag
//
LOCUS       DASVOR010001192         7072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_538137,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001192 DASVOR010000000
VERSION     DASVOR010001192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538137"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VF516_22610"
     CDS             <1..584
                     /locus_tag="VF516_22610"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8110550.1"
                     /translation="GVAHRGVLGQRARLFGPGGHSSKADHLPKPIAKLARFAAALDDA
                     GIRRLHDGPPGMQGTCLNIAAIHGGVAFNVIPARGELAWSLRPYPGFDRAGWDREVAA
                     LAARIDSDISIEHTIDHVPFSCDALVEHVRRFVRSVGTLDFWTEAALWAQHGKDAIVI
                     GPGDIAQAHAADEFVALDDLDWAVGLFRSLVRH"
     gene            complement(628..1230)
                     /locus_tag="VF516_22615"
     CDS             complement(628..1230)
                     /locus_tag="VF516_22615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110551.1"
                     /translation="MGKWLAGAIALLVLVVLLWMQIRSPDPDPVVAEHAAAPAAAVPA
                     VVPAAAKPAAQAAQAPQAAPEDELDLPERSGKIDPRSDEFFTRFDEVVPRELTRNAAR
                     CYEGRHGQLHRNQGLKLKYKIRIVNGQVTVRDVTVKESTLDDPALETCFIQEVARSTW
                     KDDDLPDWEQDDELVLRPERGMKKYWRENVDYVGPEAPRM"
     gene            complement(1249..1836)
                     /locus_tag="VF516_22620"
     CDS             complement(1249..1836)
                     /locus_tag="VF516_22620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110552.1"
                     /translation="MTRLSGVHVVFALVAASAGCGVGGGDGGTTPPGGTPVTPVNDGQ
                     LCEAHFTITGTFTPGTAPRPTDPDTGLPLTACWPVGQWSFTATVSDSNCSSPPAVLSS
                     YSFTVDSVPSSDGTGNDQVLTNTTTVGSMKAHLAMSLNGQGCQGALELGSADGKDYWN
                     MKPTLSKDPADRALAGEGDYAEFKSDSWPWKSTSP"
     gene            2019..3140
                     /locus_tag="VF516_22625"
     CDS             2019..3140
                     /locus_tag="VF516_22625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110553.1"
                     /translation="MLRRFPVLATALALMSCNEASGNEVTRLRPAPVTPSADAPKVVD
                     EAGPHPADIREDAEQIAETLQRTRNKARPDDAGDWVPKEYGSGMSRWKDTGVYVDGQP
                     RGFLTFGELPIGCKPTWDRVKMSAEKHNASEPGWKWGRERHYRFTDYLRAIGINPGAV
                     NELHVYGPKLTETLIATGRDLVSPAANQFWFTFGGIVEGKPLPHAPAGFGHGRVGDKI
                     SGVMIYLKKKPPTLTDDGLFLDGEEQLGVPYYGEPVRGGVRVYLDDKLATIIKRQDLD
                     PKQGTSGADGQPRWRLVDFLTRHGVDVSHVAELWTIQNDTRGAKFSRSELAALTFQAS
                     SQSRGGILLGDKEIVANAIALHSRPLQPSDLPVQIQDDE"
     gene            3513..5402
                     /locus_tag="VF516_22630"
     CDS             3513..5402
                     /locus_tag="VF516_22630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEX8110554.1"
                     /translation="MTNKKLVMFAVAGVVPALHGVVHAESQGAVVSLINPAPTKDLAP
                     VTGTPARLKRTDEEQPGFEMPAFAFFTNPDGSLPPNPKGLYFAMATELNGVVAKNRMQ
                     LSSTPFQLMQDGSGAISAVSDQTLAKFVTNNVDTNEHRNANAPTAMTINNGTAIVVHY
                     NVQLRGTNDTKRYIMAFNQNGDVLLPQTEEMAKNNDDCAMHQDGAPGVAIDRGDTAQG
                     HVTQLVQWGGCNGNGRDNGWAWVSTVTCNSATAPTSCTFAKTFDVSMEPNEERSRGRC
                     SVSTDTSYAVCTWTAGNNQPERNGVWIGAISLDPSIKGTDQQKAILWKMQVAGRVDAN
                     LAANGQRTYAQRAIHERIMVPNATTGALEPSDKIMFRYGDAVGRNNNKGTYFTNKVAV
                     IQVDKTQANVKYLVQPTDLTNQLPGLGGTHLGSTAAVFGPNATPGLVFLGGSLFGGGT
                     PSMLAMVSADPASNYALKTVGQMSGAPHDAHLYSNYLGGNPGNQGRNHSQMMVIANPF
                     FGKNGSTSKNLLLTATTAKTTATTYPGAMDTMSNPAIKLSALMTVLPMGGGGTAMGNG
                     TGGGNGSGANNGSGTGNGSNAGSDNSGGSDPGTTLGGCSATGGTTGLATFLLIGLAAL
                     IRRRR"
     gene            5446..6327
                     /locus_tag="VF516_22635"
     CDS             5446..6327
                     /locus_tag="VF516_22635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110555.1"
                     /translation="MRNILLAVVAATGLIGCVGGIDGTTPPPGPGPGPGPGPGSGDPG
                     TVVGTAQSKQLFDTNVYPILSSKCMSCHNATSPLAGAPGFVAPSATDGYQVASSTVAL
                     IGDLKPADAHILTTVAAGHNGVTYSPTDITSITNWLEAELNARQGGAPGSTDLLSQFS
                     GCLTQTDFDTAKMATSWANMQLNNGSRAACGACHDNFEYNQIATTVSTKFFMFISTNS
                     VAMSQYFTLDSPTAPTKVIINEKHLQDVATPAPAYTGHGQFTLTNSAGMKALTAFYNA
                     ATAKLAAGTCGPPTLTQ"
     gene            6425..>7072
                     /locus_tag="VF516_22640"
     CDS             6425..>7072
                     /locus_tag="VF516_22640"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110556.1"
                     /translation="MADLVSGDGRTHIHSRLMRESLQNRRVLSQTDSDRDLRVFPDVT
                     LVSIGGQSIFDRGKAAILPLVEELVELKRDRSLKMVIGVGGGTRVRHTISIALDLGLP
                     VGGMAQLVGAMEELNAILLNTLLAPHGSMPMQRDHFWDLPLYFDAGITPIAISVPPYH
                     FWEPPPDAGHLPMHGSDFGLFQLAEVLGMKQVIYVKDEDGLYDRDPKKHPGARKFE"
BASE COUNT         1196 a         2590 c         2298 g          988 t
ORIGIN      
        1 cgggcgtcgc gcatcgcggc gtgctcggcc agcgcgcgcg gctgttcggc cccggcgggc
       61 actcgtcgaa ggccgaccac ctgcccaagc cgatcgccaa gctcgcccgg ttcgccgccg
      121 cgctcgacga cgccgggatc cggcggctcc acgacgggcc gcccggcatg cagggcacct
      181 gcctcaacat cgccgcgatc cacggcggcg tcgcgttcaa cgtgatcccg gcgcgcggcg
      241 agctcgcgtg gtcgctccgg ccctaccccg gcttcgaccg cgccggctgg gatcgcgagg
      301 tcgccgcgct cgccgcccgg atcgactccg acatctcgat cgagcacacg atcgaccacg
      361 tgccgttctc gtgcgacgcg ctggtcgagc acgtgcgccg gttcgtgcgc tcggtcggca
      421 cgctcgactt ctggaccgaa gccgcgctgt gggcgcagca cggcaaggac gcgatcgtga
      481 tcggcccggg cgacatcgcg caggcccacg cggccgacga gttcgtcgcg ctcgacgatc
      541 tcgattgggc ggtcggcctg ttccgctcgc tcgtccggca ctgacccacc gaccgcgagc
      601 cggctccggc ggccgcgacc gccgcgatca catccgcggc gcttcggggc cgacgtagtc
      661 gacgttctcg cgccagtact tcttcatgcc gcgctcgggg cgcagcacga gctcgtcgtc
      721 ctgctcccag tcagggagat catcgtcctt ccacgtcgag cgcgcgacct cctggatgaa
      781 gcaggtctcg agcgccggat cgtcgagcgt cgactccttg accgtgacgt cccggaccgt
      841 gacctggccg ttgacgatcc ggatcttgta cttgagcttg aggccctggt tgcggtggag
      901 ctggccgtgc cgcccctcgt agcaccgggc cgcgttgcgg gtcagctcgc ggggaacgac
      961 ctcgtcgaac cgggtgaaga actcgtcgct cctcggatcg atcttgccgg accgctcggg
     1021 tagatcgagc tcgtcttcag gagcagcctg gggggcctgc gcggcctgcg cggccggctt
     1081 cgccgcggcg ggaaccaccg cgggcaccgc agcggcaggt gccgcggcat gctcggccac
     1141 gaccggatcc gggtcaggcg accggatctg catccacaac aggaccacga gcaccaggag
     1201 cgcgatcgcc ccggccagcc atttgcccat gacggtgccc tcgtggcgct acgggctcgt
     1261 gctcttccac ggccaggagt cggacttgaa ctcggcgtag tcgccctcgc cggcgagcgc
     1321 gcggtccgcc ggatccttcg acagcgtcgg cttcatgttc cagtagtcct tgccatcggc
     1381 gctgccgagc tcgagagcgc cctggcagcc ctgaccgttc agcgacatcg ccaggtgcgc
     1441 cttcatgctc ccgacggtgg tcgtgttggt cagcacctgg tcgttgccgg taccgtcgga
     1501 ggacggcacg ctgtccaccg tgaacgagta gctggagagc accgcggggg gactggagca
     1561 gttgctgtcc gacaccgtgg cggtgaagct ccactgcccc accggccagc acgcggtgag
     1621 cggcagcccg gtgtcgggat cggtcggccg cggcgcggtg cccggcgtga acgtccccgt
     1681 gatggtgaag tgcgcctcgc agagctgacc gtcattgacg ggcgtgaccg gcgtcccacc
     1741 cggcggtgtg gttccaccgt cgccgccgcc cacaccgcac ccggccgagg cagcgacgag
     1801 cgcgaagacc acatggaccc cagagagtct tgtcatgcgg ccatcgcgtt gcaatgacgg
     1861 cgccaagccc gaagtcgttg aatcctcgcc gaggatggcc ctgacaaccc cgccagatgg
     1921 ggtgaggggt ggtgagtcgc gacctcgccg aggaccccgc cccgttcttt acgaaccgca
     1981 cgttgcgcgc tggaacgggc gctgcacaca atggcgccat gctccgccgc ttcccggtgc
     2041 tggccaccgc gctggctctg atgtcctgca acgaagccag cggtaacgag gtcacgcggc
     2101 tccgcccggc gcccgtcacg ccgtcggccg atgcgcccaa ggtcgtcgac gaagccggtc
     2161 cgcatccggc cgacattcga gaagacgccg agcagatcgc agagaccctc cagcggaccc
     2221 ggaacaaggc ccggccggac gacgcggggg actgggtgcc caaggagtac ggctccggca
     2281 tgtcgcgctg gaaggacacc ggcgtctacg tcgacggcca gccgcgcggc ttcctcacgt
     2341 tcggcgagct gccgatcggg tgcaagccga cctgggaccg ggtcaagatg tccgcggaga
     2401 agcacaacgc gagcgagccg ggctggaagt ggggacgcga gcggcactac cggttcaccg
     2461 actacctccg cgcgatcggc atcaaccccg gcgccgtcaa cgagctgcac gtctacggcc
     2521 cgaagctcac cgagaccctg atcgcgaccg ggcgcgatct ggtgtcgccg gcggcgaacc
     2581 agttctggtt cactttcggc gggatcgtcg agggcaaacc cctgccgcac gcacccgcgg
     2641 gattcggcca tggaagggtc ggcgacaaga tcagcggggt catgatctac ctcaagaaga
     2701 agccgccgac gctgaccgac gacgggctgt tcctcgacgg cgaggagcag ctcggcgtgc
     2761 cctactacgg ggagccggtg cgcggcggcg tgcgggtcta cctcgacgac aagctggcga
     2821 cgatcatcaa gcgtcaggat ctcgacccca agcaagggac gtccggggcc gatggccagc
     2881 cgcggtggag actcgtggac ttcctcaccc gccacggcgt cgacgtctcg cacgtggccg
     2941 agctgtggac gatccagaac gacacgcgcg gggcgaagtt ctcgcgcagc gagctcgccg
     3001 cgctgacctt ccaggccagc tcgcagtccc ggggcggcat cctgctcggc gacaaggaga
     3061 tcgtcgccaa cgcgatcgcg ctgcacagcc gcccgctcca gccgtccgac ctgcccgtcc
     3121 agatccagga cgacgagtag tctccgcgcc cgcgatgtcg ggcgccgtcg ccgccgctgc
     3181 gacccggggc gtggcgccgc gtgcttcgct ccgcgaaccc cgccggacga gtcgccgcga
     3241 tgcgcgggac ggccgggtga tgcgtccaca cccaccgacg gggggcgcgg ccccagatcc
     3301 gactgcgagc gagtgtcggc cagatccgga cagtgcgcct tacctcgcga gatcagcgcc
     3361 ggtttttgga accgcacccc caggcgcgga gcggttaccc ccatgtttca tcggcatcac
     3421 atgccgtgat tcctcggggt tacgctctcg ccgcgacggc atgtgccttg ctcaaaagcg
     3481 tcgacatcac aacgaacagg gcagccaacc aaatgaccaa caagaagctc gtgatgttcg
     3541 ccgtggccgg ggtcgtcccc gcgctccacg gggtcgtcca tgcggagtcc cagggtgcgg
     3601 tggtatcgct gatcaacccc gctccgacca aggacctcgc accggtgacc gggaccccgg
     3661 cgcgcctgaa gcgtaccgac gaggaacagc cgggcttcga gatgccggcg ttcgcgttct
     3721 tcaccaaccc ggacgggtcg ctgcccccga accccaaggg gctctatttc gcgatggcca
     3781 ccgagctcaa cggcgtggtt gcgaagaacc ggatgcagct gtcctccacc ccgttccagc
     3841 tgatgcagga cgggagcggc gcgatctcgg ccgtgtccga ccagaccctg gccaagttcg
     3901 tcaccaacaa cgtggacacc aacgagcatc gcaacgccaa cgcgccgacc gcgatgacga
     3961 tcaacaacgg caccgccatc gtcgtccact acaacgtgca gctgcgcggg accaacgaca
     4021 ccaagcgcta catcatggcg ttcaaccaga acggtgatgt gctcctgccg cagaccgagg
     4081 agatggccaa gaacaacgac gactgcgcga tgcaccagga cggcgcgccc ggcgtcgcga
     4141 tcgatcgcgg cgacaccgcg cagggccacg tcacccagct cgtgcagtgg ggcggttgca
     4201 acggcaacgg ccgcgacaac ggctgggcct gggtcagcac cgtgacctgc aacagcgcca
     4261 cggcgccgac gtcgtgcacg ttcgccaaga ccttcgacgt ctcgatggag cccaacgagg
     4321 agcgctcgcg cggccggtgc tcggtgtcga cggacacgag ctacgcggtg tgcacgtgga
     4381 cggccggtaa taaccagccg gagcgcaacg gcgtctggat cggcgcgatc tcgctggatc
     4441 catcgatcaa gggcacggac cagcagaagg ccatcctgtg gaagatgcag gtcgccggcc
     4501 gggtcgacgc caacctcgcc gccaacggtc agcggacgta cgcccagcgc gccatccacg
     4561 agcgcatcat ggtgccgaac gcgaccaccg gcgcgctcga gccgagcgac aagatcatgt
     4621 tccgctacgg tgacgccgtc ggccgcaaca acaacaaggg cacctacttc accaacaagg
     4681 tggccgtgat ccaggtcgac aagacccagg ccaacgtgaa gtacctggtc cagccgaccg
     4741 acctgaccaa ccagctcccc ggcctcggcg gcacgcacct cggctcgacc gcggccgtgt
     4801 tcggccccaa cgccaccccg ggcctggtgt tcctcggcgg ctcgctgttc ggcggcggca
     4861 ccccctcgat gctcgccatg gtctcggccg acccggccag caactacgcg ctgaagaccg
     4921 tcggtcagat gtcgggcgcg ccccacgacg cccacctcta ctcgaactac ctcggcggca
     4981 accccggtaa ccagggccgc aatcacagcc agatgatggt catcgccaac ccgttcttcg
     5041 gcaagaacgg cagcaccagc aagaacctgc tgctcaccgc caccaccgcc aagaccacgg
     5101 ccaccaccta cccgggcgcg atggacacga tgtcgaatcc ggcgatcaag ctgtccgccc
     5161 tcatgacggt gctgccgatg ggcggcggcg gcaccgccat gggcaacggc accggcggcg
     5221 gcaacggcag cggcgccaac aacggcagcg gcaccgggaa cggcagcaac gccggcagcg
     5281 acaacagcgg cggcagcgac ccgggcacca cgctcggtgg ctgcagcgcg acgggcggca
     5341 cgaccggcct ggcgacgttc ctcctcatcg gcctggcggc gctcatccgc cgccgccgct
     5401 gagctccccc ccctactcca cacctgaacc cagcgaggag ctcagatgcg aaacatcctc
     5461 ctagcagttg tcgcggcgac cggcctcatc ggctgcgtcg gcggtatcga cggcaccacg
     5521 cctccccccg gccccggccc cggccccggc cctggccccg gctcgggcga tcccggcacc
     5581 gtcgtgggca cggcacagtc caagcagctg ttcgacacca acgtctatcc gatcctgtcg
     5641 agcaagtgca tgagctgcca caacgccacg agcccgctcg ccggcgcccc tggcttcgtc
     5701 gcgcccagcg ccaccgacgg ctaccaggtc gccagcagca ccgtcgcgct gatcggtgat
     5761 ctgaagccgg ccgacgccca catcctgacg acagtcgcgg ccggccacaa cggcgtgacc
     5821 tactcgccga ccgacatcac cagcatcacg aactggctcg aggccgagct caacgcgcgc
     5881 cagggcggcg ccccgggcag caccgacctg ctcagccagt tctcgggatg cttgacccag
     5941 accgacttcg acaccgcgaa gatggccacg tcgtgggcga acatgcagct caacaacggc
     6001 tcgcgcgccg cgtgcggcgc ctgccacgac aacttcgagt acaaccagat cgccacgacg
     6061 gtgagcacga agttcttcat gttcatcagc acgaacagcg tcgcgatgtc gcagtacttc
     6121 acgctcgact cgccgaccgc gccgaccaag gtgatcatca acgagaagca cctgcaggac
     6181 gtcgcgacgc cggccccggc gtacaccggc cacggtcagt tcaccctgac caacagcgcg
     6241 gggatgaagg cgctgacggc gttctacaac gcggccaccg cgaagctggc cgccggcacc
     6301 tgcggcccgc cgaccctcac gcagtgacct gaggtcagac ggtcgcagca tggtgcccgc
     6361 cgagaggttg gcaccatggt tgttttcggc gggtcggttt cggcgctatc ctcgggacct
     6421 gtccatggca gacctcgtca gcggcgatgg ccgcacccac atccactcgc ggctgatgcg
     6481 cgagtccctg cagaaccgcc gcgtgctgtc gcagaccgac agcgaccgcg atctgcgcgt
     6541 gttccccgac gtcaccctgg tctcgatcgg agggcagagc atcttcgacc gcggcaaggc
     6601 agcgatcctg ccgctggtcg aggagctggt cgagctcaag cgcgaccgca gcctgaagat
     6661 ggtgatcggg gtcggcggcg gcacccgggt ccgccacacg atctcgatcg ccctcgacct
     6721 cggcctgccg gtcggcggca tggcccagct ggtcggcgcg atggaggagc tcaacgccat
     6781 cctgctcaac accctgctcg cgccgcacgg ctcgatgccg atgcagcgcg atcacttctg
     6841 ggacctgccg ctgtacttcg acgccgggat cacgccgatc gcgatctcgg tgccgcccta
     6901 ccacttctgg gagccgccgc ccgacgccgg tcacctgccg atgcacggct cggacttcgg
     6961 cctgttccag ctcgccgagg tcctgggcat gaagcaggtc atctacgtca aggacgagga
     7021 cggcctgtac gaccgcgatc ccaagaagca ccccggcgcc cgcaagttcg ag
//
LOCUS       DASVOR010001193         4072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_128752,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001193 DASVOR010000000
VERSION     DASVOR010001193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4072
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128752"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VF516_22645"
     CDS             complement(<1..590)
                     /locus_tag="VF516_22645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8110557.1"
                     /translation="MTDSPMSSIKVLLIEDDERLAQLTARYLESHGVLVTISPTGPEG
                     QVEALRRQYDCIVLDVMLPGRDGIEVTRELRARTDVPILMVTARTEEADRVLGLEVGA
                     DDYVTKPFSPRELLARIRATVRRVRGQAGPTQQTVQVGGLVLDPQKMTVTLDGNPIDV
                     TAYEFSILRALAQRPGRVLSREQLLDLAKGSAELSF"
     gene            complement(688..2547)
                     /locus_tag="VF516_22650"
     CDS             complement(688..2547)
                     /locus_tag="VF516_22650"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110558.1"
                     /translation="MSLRDRELYRFFERSPIGMFRCDEPGRFLYVNPSLVRMLGYDSA
                     EELLEKNIARDVYVDPEDRTRLLARYRHKGGVDGARVRWKTKQGRERIVLLYSQVVEE
                     PEATYLDTSVLDITDAENVTLELRKQRETLETTAAMLDLVVRQMQAVYWVVDRDLRIC
                     RSGGAVRDLFGIPPARFLGMTLEQVHRADPGSHDPVGMHRRALIGETVTYTNEWRQKQ
                     LVTTVCPHRSNGVIVGAIGTCVDMTTQYMLERRMVDAQRAESLGVLAGGLAHDFNNLL
                     VAILGNADLALREIGPRGPGRGPLENIQQAGLRGAELIEQLLAYAGHRGVASTRVSPT
                     PVIEELLRIIAPTIPRNIELRVEIASDLVLRGDAPQIRQVLLNLLNNARDALGSRGGT
                     IAIIGRLLRHDGAAHPDDVIAAPAGTYVELEIADDGPGMSHETRRRIFEPFFTTKSTG
                     HGLGLAAVAGIVRAHGGGMRLATAPGEGARFQVQWPSTFTPSELTAVSPPANGHTILI
                     IDDEDLVRDVVARMIEDLGYAAITATDGPAGLAIVDSLPIDAVLVDLTMPRMGGLDVV
                     NALRARRPDLPIVLCSGFDRDVRSIRADAYLPKPFRIEALESALARLLPPRSS"
     gene            complement(2605..3726)
                     /locus_tag="VF516_22655"
     CDS             complement(2605..3726)
                     /locus_tag="VF516_22655"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEX8110559.1"
                     /translation="MAVQRIGILTSGGDAPGMNAAIRAATLVARAQGVDVVGIERGYR
                     GLLDGAFIPLAPADVAGILREGGTILGSARCKEFHHREVRDRARAALAARGIDGLIVI
                     GGNGSLAGAHRLADPDELAGQPLAIAGVPASIDNDLGLTGLAIGVDTAMNTIVEACDK
                     IADTATAHDRTFIVEVMGRDCGYLAMTSAIAVGADLALFPEAGKPEAAIVEQIVDTVL
                     TVRRRARRTRRVIIIKAEGVAIAIDRLKASVDARLRERAPDADPAGIETRVTVLGHVV
                     RGGRPSAFDRLLGSRLANVAVRALLGGVSHRMASWMPTMPAAELAPDIASRSPVDPYC
                     WLVDLGAVLAETENLLRGHSSLARWRAGAFAEIEDALLL"
     gene            3845..>4072
                     /locus_tag="VF516_22660"
     CDS             3845..>4072
                     /locus_tag="VF516_22660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8110560.1"
                     /translation="MVRTEREGPVTTVILDRAAAQNAADRATAEQLAAAFREFDADAD
                     ARVAVLYGDHGAFCAGADLQAMATGAGNRVAP"
BASE COUNT          562 a         1449 c         1474 g          587 t
ORIGIN      
        1 tcgaagctga gctcggccga gcccttggcg agatcgagca gctgctcgcg cgacagcacg
       61 cggccggggc gctgggccag cgcgcgcagg atcgagaact cgtaggcggt gacgtcgatc
      121 gggttgccgt cgagcgtcac cgtcatcttc tggggatcga gcaccaggcc gccgacctgg
      181 acggtctgct gggtcggccc ggcctggccg cggacccggc gcaccgtcgc gcggatccgg
      241 gcgagcagct cgcgcggcga gaacggcttg gtcacgtagt cgtcggcgcc gacctcgagg
      301 ccgagcacgc ggtcggcctc ctcggtgcgc gccgtgacca tcaggatcgg cacgtcggtc
      361 cgcgccctga gctcgcgggt gacctcgata ccgtcgcgac cgggcagcat gacgtcgagc
      421 acgatgcagt cgtactgccg gcgcagcgcc tcgacctggc cctcggggcc ggtcggcgag
      481 atcgtcacca gcacgccgtg cgactcgaga tagcgcgcgg tcagctgggc gaggcgctcg
      541 tcgtcctcga tcagcagcac cttgatcgag ctcatcggac tgtccgtcac aggcacaacc
      601 taaccatggc gcgggaattc tgcgcccggg gttcacactt ctcaactcga ttgcaaccgg
      661 gtagtcaccg gccagaccgc ccgcgggtca cgacgaccgc ggcggcagca gccgggccag
      721 cgcgctctcc agggcctcga tccggaacgg cttgggcaag tacgcgtcgg cgcggatcga
      781 gcggacgtcg cggtcgaacc cgctgcacag cacgatcggc aggtcgggcc ggcgcgcgcg
      841 cagcgcgttg accacgtcga ggccgcccat ccgcggcatg gtcaggtcga ccagcaccgc
      901 gtcgatcggc aggctgtcga cgatcgccag accggccggt ccgtcggtcg cggtgatcgc
      961 ggcgtagccg aggtcctcga tcatccgggc cacgacgtcg cgtacgagat cctcgtcgtc
     1021 gatgatcagg atggtgtggc cgttggccgg cggcgacacc gcggtgagct cgctcggcgt
     1081 gaacgtcgac ggccactgga cctggaaccg cgcgccctcc cccggcgcgg tggccagccg
     1141 catgccgccg ccgtgcgcgc gcacgatgcc ggcgaccgcg gccaggccca ggccgtggcc
     1201 ggtcgacttg gtcgtgaaga acggctcgaa gatccggcgc cgggtctcgt ggctcatccc
     1261 ggggccgtcg tcggcgatct cgagctcgac gtaggtcccg gcgggcgcgg cgatcacgtc
     1321 gtcggggtga gccgcgccgt cgtggcggag cagccggccg atgatcgcga tcgtgccgcc
     1381 gcggctgccg agcgcgtcgc gcgcgttgtt cagcagattg agcaggacct ggcggatctg
     1441 cggcgcgtcg ccgcgcagca ccaggtcgct cgcgatctcg acccggagct cgatgttgcg
     1501 cggtatggtc ggcgcgatga tccggagcag ctcctcgatc accggggtcg gcgacacccg
     1561 ggtcgacgcc acgccgcggt ggccggcgta ggccaggagc tgctcgatca gctcggcgcc
     1621 gcgcagcccg gcctgctgga tgttctccag cggaccgcgg cccggcccgc gcggcccgat
     1681 ctcgcgcagc gcgaggtcgg cgttgccgag gatcgcgacc agcaggttgt tgaagtcgtg
     1741 ggcgagcccg ccggcgagca cgccgaggct ctcggcgcgc tgggcgtcga ccatgcggcg
     1801 ctccagcatg tactgcgtgg tcatgtcgac gcaggtcccg atcgcgccga cgatcacgcc
     1861 gttgctgcgg tgcggacaca ccgtggtgac cagctgcttc tggcgccact cgttggtgta
     1921 ggtcacggtc tcgccgatca gcgcgcggcg gtgcatgccg accggatcgt gcgagccggg
     1981 atcggcgcgg tggacctgct cgagcgtcat gccgaggaac cgggccgggg ggatgccgaa
     2041 caggtcgcgg accgcgccgc cgctccggca gatccggaga tcgcggtcga cgacccagta
     2101 caccgcttgc atctggcgga ccacgaggtc gagcatcgcc gcggtggtct ccagcgtctc
     2161 gcgctgcttg cggagctcga gggtgacgtt ctcggcatcg gtgatgtcga gcaccgaggt
     2221 gtcgaggtag gtggcctcgg gctcctcgac gacctgcgag tacagcagga cgatccgctc
     2281 gcggccctgc ttggtcttcc accgcacgcg ggcgccgtcg accccgccct tgtgccggta
     2341 gcgcgccagc agccgggtac ggtcctcggg atcgacgtag acgtcgcgcg cgatgttctt
     2401 ctccagcagc tcctcggcgc tgtcgtagcc cagcatccgg accagcgagg gattcacgta
     2461 gaggaaccgg cccggctcgt cgcaccggaa catgccgatc ggggaccgtt cgaagaacct
     2521 gtacagctcg cgatcccgca acgacacgct gggagcatca tatcccacaa ccgccgcgct
     2581 ggcgcacggc gcgccgcgac atgactaaag cagcagcgcg tcttcgatct ccgcgaatgc
     2641 cccggcgcgc cagcgcgcca gcgagctgtg gccgcgcagc aggttctcgg tctctgcaag
     2701 caccgcaccg agatcgacca gccagcagta cgggtcgacc ggcgatcggc tcgcgatgtc
     2761 gggcgcgagc tcggccgcgg gcatcgtcgg catccatgat gccatccggt ggctgacccc
     2821 gcccagcagc gcgcggaccg cgacgttggc cagccggctg ccgagcaggc gatcgaacgc
     2881 cgacggccgg ccgccgcgca cgacgtggcc gagcacggtc acccgggtct cgatcccggc
     2941 ggggtcggcg tcgggcgcgc gctcgcgcag ccgggcgtcg acgctggcct tgagccggtc
     3001 gatcgcgatc gcgacgccct cggccttgat gatgatcacg cggcgggtcc gccgcgcccg
     3061 ccggcgcacg gtcagcacgg tgtcgacgat ctgctcgacg atcgcggcct cgggcttgcc
     3121 ggcctcgggg aacagcgcga gatcggcgcc gaccgcgatc gccgaggtca tcgccaggta
     3181 gccgcagtcg cgccccatca cctcgacgat gaacgtccgg tcgtgcgcgg tcgcggtgtc
     3241 ggcgatcttg tcgcacgcct cgacgatcgt gttcatcgcg gtgtcgacgc cgatcgccag
     3301 gccggtcagc ccgaggtcgt tgtcgatcga cgccgggacc ccggcgatcg cgagcggctg
     3361 gccggcgagc tcgtcgggat cggcgagccg gtgcgcgccg gccagcgagc cgttgccgcc
     3421 gatcacgatc aggccgtcga tcccgcgggc cgccagcgcc gcgcgcgccc ggtcgcggac
     3481 ctcgcgatgg tggaactcct tgcagcgcgc cgagccgagg atcgtgccgc cctcgcgcag
     3541 gatgcccgcg acgtcggccg gggcgagcgg gatgaacgcg ccgtcgagca ggccgcgata
     3601 gccgcgctcg ataccgacga cgtcgacgcc ctgcgcccgc gcgaccaggg tcgccgcgcg
     3661 gatcgccgcg ttcatcccgg gagcgtcacc gccgctggtc aggatgccga tgcgctggac
     3721 tgccatgttg ggactctacg acgtcatcca ggttgcctgt cattgtgatc cgaatagctc
     3781 gcaggtattt tccgtcgcaa tttccgccgg tccggcgacc gagctcggtt acactgcgcg
     3841 gaccgtggtt cgtaccgagc gcgagggtcc tgtcaccact gtcatcctcg atcgcgccgc
     3901 ggcgcagaac gcggccgatc gcgccaccgc cgagcagctc gccgcggcgt tccgggagtt
     3961 cgacgccgac gccgacgcgc gggtcgccgt gctgtacggc gatcacggcg cgttctgcgc
     4021 cggcgcggat ctgcaggcga tggcgaccgg cgccggcaac cgggtcgcgc cc
//
LOCUS       DASVOR010001194         2570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_450282,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001194 DASVOR010000000
VERSION     DASVOR010001194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450282"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF516_22665"
     CDS             complement(<1..293)
                     /locus_tag="VF516_22665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110561.1"
                     /translation="MTARLPPSRALAGAGAIAAAAGLWWSDTPLGAMLWTFALVAACV
                     GYGDQLARLLGDRLTIGASAVTGLACLIVGSTLLAAAGGLNRAIELGLVGLGLA"
     gene            complement(290..>2570)
                     /locus_tag="VF516_22670"
     CDS             complement(290..>2570)
                     /locus_tag="VF516_22670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110562.1"
                     /translation="KAIAAIGLGLALRELVVRARSPERRPADPTPPRILYLVLGGVLV
                     VYLALLVLGSIASHGNPFDDYVGYLALTRRMLDCGDLIEPFSFRRISAYGGQTALHAL
                     VALRGDVASGDLLDTGMFQAIAVLVIVDLARRRRLHVAATALIVAFVASLPDLSINAA
                     ATWTGVAMFLCAYSLASDPARDGRALVLTFAVCAGACTLRQNYLLPAGGFAALLLVFQ
                     ARARAAAASWRTAWAEQRGTAALCVAVAAGVLVPYAIATWRSNATFLYPLVLGTANPA
                     APLRPTGFTVFDELSFWFAVALDTEPIRIWWVLAPFMLVARDARAARPWAALLIASVV
                     GFAVLVHSFALSDTATLWRYGFGYMTPLAVVFLIEAGARLPLVDPRDQPEPQLRLPAL
                     AVFFVWLAVLTQFVQARVTPERRIALLAENVPAGLARGTEKAGDPALYRELQAALPEG
                     ARVAVLLDDPYLLDYGRNDIVNLDLPGFAAPRPGLPSFLGAERWRGYLRSLGIRYAAF
                     VDGEFSSYLFRRRSWVPRIFTDSELFRFMGAHMVDALDSLRELARTSRVVFHRAGITA
                     IDLGEPVPADAADPARDEPERVRQDAFIRHLAETELHNAAWQLASRRDVVFVRDGAGP
                     SVVQVELPETTSPFTRSLLEQVTGKPLPPPPPCRWLSERTHVRVLGSGRHHLHVALRV
                     DVARLASIPSARLSIDGAPIGRASPDAQGYLGFDAAVSCTGWCDVYVVFSTASEYWAS
                     AESVRGIQLLGFDWTSEPR"
BASE COUNT          398 a          949 c          966 g          257 t
ORIGIN      
        1 gccagcccga gcccgaccag cccgagctcg atcgcgcggt tcaggccgcc ggcggccgcg
       61 agcagcgtcg agccgacgat caggcacgcc aggccggtca cggcggatgc gccgatcgtc
      121 agccggtcgc cgagcagccg ggccagctga tcgccgtagc cgacgcacgc cgcgacgagc
      181 gcgaacgtcc acagcatcgc gccgagcggc gtgtccgacc accacagccc ggccgccgcg
      241 gcgatcgcac cggcgccggc cagggcccgg ctgggcggga gccgggcggt catctcggct
      301 cgctcgtcca gtcgaagccg agcagctgga tcccgcgcac cgactcggcg gacgcccagt
      361 actccgaggc ggtcgagaac accacgtaga cgtcgcacca gccggtgcac gacaccgcgg
      421 cgtcgaaccc gaggtagccc tgggcatccg ggctggcgcg gccgatcggc gcgccgtcga
      481 tcgacagccg cgcgctcggg atcgaggcga gccgggcgac gtcgacccgc agcgcgacgt
      541 gcaggtggtg gcggccggat ccgagcaccc gcacgtgggt gcgctcgctc agccagcggc
      601 acggcggggg cggcggcagc ggcttgccgg tcacctgctc gagcagcgac cgggtgaacg
      661 ggctggtcgt ttcgggcagc tcgacctgca ccaccgacgg cccggcgccg tcgcgcacga
      721 acaccacgtc gcggcgcgac gcgagctgcc aggccgcgtt gtgcagctcg gtctccgcga
      781 ggtggcggat gaacgcgtcc tggcggaccc gctcgggctc gtcgcgcgcg ggatcggcgg
      841 cgtcggcggg gaccggctcg ccgaggtcga tcgccgtgat cccggcgcgg tggaacacca
      901 cgcgcgaggt ccgggcgagc tcgcgcaggc tgtcgagcgc gtcgaccatg tgcgcgccca
      961 tgaaccggaa cagctcgctg tcggtgaaga tccggggcac ccacgaccgc cggcggaaca
     1021 ggtagctcga gaactcgccg tcgacgaacg cggcgtagcg gatgccgagc gaccgcaggt
     1081 agccgcgcca gcgctccgcg cccaggaagc tcggcagccc gggccggggc gcggcgaacc
     1141 ccgggagatc gaggttgacg atgtcattgc gcccgtagtc gagcaggtac ggatcgtcga
     1201 gcaggaccgc gacccgcgcg ccctcgggca gggcggcctg gagctcgcgg tacagcgccg
     1261 ggtcccccgc cttctcggtg ccgcgcgcca gcccggccgg cacgttctcg gcgagcaggg
     1321 cgatccggcg ctccggtgtg acccgggcct ggacgaactg ggtcagcacg gcgagccaga
     1381 cgaagaacac cgcgagcgcg ggcagccgga gctgcggctc gggctggtcg cgcgggtcga
     1441 ccagcggcag ccgcgcgccg gcctcgatca ggaacaccac cgcgagcggg gtcatgtagc
     1501 cgaagccgta gcgccacagc gtcgcggtgt ccgacagcgc gaacgagtgg acgaggaccg
     1561 cgaaccccac gaccgacgcg atcagcagcg ccgcccacgg ccgggcggcc cgcgcgtcgc
     1621 gcgccaccag catgaacggc gcgagcaccc accagatccg gatcggctcg gtgtcgagcg
     1681 cgaccgcgaa ccagaagctc agctcgtcga acacggtgaa cccggtcggc cgcagcggcg
     1741 ccgccgggtt ggcggtgccg agcacgagcg ggtagaggaa cgtcgcgttc gaccgccagg
     1801 tcgcgatcgc gtagggcacc agcacaccgg cggcgacggc gacgcacagc gctgcggtgc
     1861 cgcgctgctc ggcccacgcc gtgcgccacg acgcggcggc agcgcgcgcc cgcgcctgga
     1921 acaccagcag cagcgcggcg aacccgccgg ccggcaacag gtagttctgg cgcagcgtgc
     1981 acgccccggc gcagaccgcg aacgtcagca ccagcgcgcg cccgtctcgc gccggatccg
     2041 acgccaggct gtaggcgcac aggaacatcg cgaccccggt ccaggtcgcc gccgcgttga
     2101 tgctgaggtc gggcagcgac gcgacgaacg cgacgatcag cgcggtggcg gcgacgtgca
     2161 gccggcggcg gcgtgcgaga tcgacgatca ccagcaccgc gatcgcctgg aacatgccgg
     2221 tgtcgagcag gtcccccgac gcgacgtcgc cgcgcagcgc gaccagcgcg tgcagcgcgg
     2281 tctggccgcc gtacgcgctg atccgccgga agctgaacgg ctcgatcagg tcgccgcagt
     2341 cgagcatgcg ccgggtcagc gccaggtagc cgacgtagtc gtcgaacggg ttgccgtgcg
     2401 acgcgatcga tccgagcacc agcagcgcga ggtacaccac cagcacgccg ccgagcacca
     2461 ggtagaggat gcgcggcggc gtcgggtccg ccgggcggcg ctccgggctg cgcgcgcgga
     2521 ccaccagctc gcgcagcgcg agcccgagcc cgatcgccgc gatcgctttt
//
LOCUS       DASVOR010001195         1875 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_41439,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001195 DASVOR010000000
VERSION     DASVOR010001195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1875
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41439"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VF516_22675"
     CDS             complement(<1..643)
                     /locus_tag="VF516_22675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110563.1"
                     /translation="MNWFTWGPRKSSADRRADAARAIQDAARRGQALSPVAIAGRTIA
                     QTFWGKAWCGNLERYRDFAYRLERGRSYVRSGSVIDLRIAAGKIAARVSGSSLYDVAI
                     EIDAVGRGAWGAIQRDCAGGIGSRIDLLAGKLSQSVMARLCADRTGLFPAPSAIRFRC
                     SCPDIARMCKHVAAAMYGVGARLDHAPELLFTLRQVSLDELLAAAVSELPAAPA"
     gene            complement(640..>1875)
                     /locus_tag="VF516_22680"
     CDS             complement(640..>1875)
                     /locus_tag="VF516_22680"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8110564.1"
                     /translation="PDGFAGGAGGKAPRGIELAAARAPALAGVDLVITSYGTLPRAPW
                     LAATRWGLAILDEAQAIKNPGTKQTRAVKALDARARVALTGTPVENRLGDLWSIFDFL
                     NPGLLGSAKQFARYTRQLDARGYGPLRELIRPYVLRRLKTDRSVITDLPDKTELTAYC
                     ALSRKQAALYQQAVDDLAAKLAATDGIERRGAVLASLLRFKQICNHPSHWLGDGDWRE
                     SDSGKLQRVRELAEVIAARQDKLLVFTQFREACEPLAGFLAGVFGRPGLVLHGNTAVK
                     DRSALVRRFQDDEDVPFFVLSLKAGGTGLNLTAASHVLHFDRWWNPAVEDQATDRAFR
                     IGQRRNVLVHKLVCRGTVEDKIDALIASKRALSRQLLEGTAELPLTELSDAELMQLVA
                     LDLDSAMMDGAAGAAEDRR"
BASE COUNT          253 a          677 c          693 g          252 t
ORIGIN      
        1 cggccggcgc ggcggggagc tcgctgaccg ccgcggcgag cagctcgtcg agcgagacct
       61 ggcgcagcgt gaacagcagc tcgggcgcgt gatcgagccg cgcaccgacg ccgtacatcg
      121 ccgcggcgac gtgcttgcac atgcgcgcga tgtccgggca gctgcagcgg aaccggatcg
      181 ccgacggcgc cgggaacagg ccggtgcgat cggcgcacag ccgcgccatc accgattgcg
      241 acagcttgcc ggcgagcaga tcgatccgcg agccgatgcc gccggcgcag tcgcgctgga
      301 tcgcccccca ggcgccgcgg ccgaccgcat cgatctcgat cgcgacgtcg tacaggctcg
      361 agccgctgac ccgcgcggcg atcttgccgg cggcgatccg caggtcgatc accgagccgg
      421 agcgcacgta gctgcgaccg cgctcgaggc ggtacgcgaa gtcgcggtag cgctcgaggt
      481 tgccgcacca cgccttgccc cagaacgtct gggcgatggt gcggccggcg atcgcgaccg
      541 gcgacagcgc ctggccgcgg cgcgcggcgt cctggatcgc gcgcgcggcg tcggcgcggc
      601 ggtcggcgga agacttgcgc ggcccccagg tgaaccagtt catcgtcggt cctccgcggc
      661 gcccgcggcg ccgtccatca tcgcgctgtc gagatcgagc gccaccagct gcatcagctc
      721 ggcgtcgctg agctcggtga gcgggagctc ggcggtgccc tcgagcagct ggcgcgacag
      781 cgcgcgcttg ctcgcgatca gcgcgtcgat cttgtcctcg accgtgccgc ggcacaccag
      841 cttgtggacc agcacgttgc ggcgctggcc gatccggaac gcgcgatcgg tggcctggtc
      901 ctcgaccgcg gggttccacc agcggtcgaa gtgcagcacg tgggacgccg cggtcaggtt
      961 gagaccggta ccgccggcct tgagcgacag cacgaagaac ggcacgtcct cgtcgtcctg
     1021 gaaccggcgg accagcgcgc tgcggtcctt gaccgcggtg ttgccgtgca gcaccagccc
     1081 cggccggccg aacacgccgg cgaggaagcc ggcgagcggc tcgcacgcct cgcggaactg
     1141 ggtgaacacc agcagcttgt cctgcctggc ggcgatcacc tcggcgagct cgcgcacgcg
     1201 ctgcagcttg ccgctgtcgg actcgcgcca gtcgccgtcg ccgagccagt gcgacgggtg
     1261 attgcagatc tgcttgaacc ggagcagcga cgccagcacc gcgccgcgtc gctcgatgcc
     1321 gtcggtcgcc gcgagcttgg ccgcgaggtc gtcgaccgcc tgctggtaca gcgccgcctg
     1381 cttgcgcgac agcgcgcagt acgcggtcag ctcggtcttg tcgggcaggt cggtgatcac
     1441 gctgcggtcg gtcttgagcc ggcgcagcac gtacggccgg atcagctcgc gcagcgggcc
     1501 gtagccgcgc gcgtcgagct gccgggtgta gcgcgcgaac tgcttggcgc tgccgagcag
     1561 cccggggttc aggaaatcga agatcgacca cagatccccc agccggttct cgaccggcgt
     1621 gccggtcagc gcgacgcggg cgcgggcgtc gagcgccttg accgcgcggg tctgcttggt
     1681 gcccgggttc ttgatcgcct gggcctcgtc gaggatcgcc agcccccagc gcgtcgcggc
     1741 gagccacggc gcgcgcggca gcgtgccgta gctcgtgatc acgagatcga ccccggcgag
     1801 cgcgggcgca cgagcggctg cgagctcgat cccacggggg gctttgcccc ccgccccgcc
     1861 ggcgaagccg tcggg
//
LOCUS       DASVOR010001196         3002 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_538172,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001196 DASVOR010000000
VERSION     DASVOR010001196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538172"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1099
                     /locus_tag="VF516_22685"
     CDS             128..1099
                     /locus_tag="VF516_22685"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX8110565.1"
                     /translation="MFPSDELRTSASQPLDDDSPDLDWTLALAGGEGVRLSEYVQRRF
                     GRRIPKQYCCLLGERSMLEHTLERLNKLTPPSRTLTVIGTDHGEIAAPQLAGRSDHVF
                     RQPSARDTGVALYVALAMIRRWHANATVTITPTDHYVVPSARYVEQVRIARDVARQLR
                     DAVVVLGVRPTEPDPELGYLTLGDRLAEIPELRRVVGFVEKPTPTRAHQLIAEGGLWN
                     TMVACGTVDALWELGRQTEPQLIDILDSFVPLVGTRDETDAIEYIYRAILPVSLSRDV
                     FERAPERLVVQQLDGVDWSDWGRPERIESVLAVRRSRALVPNRAYAP"
     gene            1096..>3002
                     /gene="glgX"
                     /locus_tag="VF516_22690"
     CDS             1096..>3002
                     /gene="glgX"
                     /locus_tag="VF516_22690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HEX8110566.1"
                     /translation="MTADATGRAAPASDAATGPAADPASGRRRQVWPGRSYPLGATFD
                     GYGTNFAVFSGVAERVELCLFDPEADRPGGGRPAEARIDLNRGIGQIRHAYLPLAGPG
                     MRYGFRVHGPWDPARGMRCNPNKLLVDPYARAISEGLHWGSALRGDDGNGGASTEDSA
                     PHTLRSVVVQPYFDWGTDRRLETPWNQTVIYEVHVKGLTMRHPDVPPHLRGTYAGLAH
                     PAIIKYLQSLGITAVELLPIHAFIHDGFLLEKGLRNYWGYHSIGYFAPHPEYASVHSA
                     SGAVAEFKHMVRTLHHAGIEVILDVVYNHTGEGNHDGPMLSFRGLDNMAYYRLVPEDL
                     SYYMDYTGTGNTLQMRSPHTLQLVMDSLRYWIEEMHVDGFRFDLASALARGLHEVDRL
                     GAFFDLIQQDPVVSRVKLIAEPWDVGDGGYQVGNFPPLWSEWNGKYRDWIRDYWRGEP
                     GSLPELGARITGSSDLYQQGGRFPHASINFVTAHDGFTLRDVVSYNEKHNEANGEGSR
                     DGESHNRSWNCGAEGDTDDPEVVALRRRQQRNFLATLFLSQGVPMVLGGDELGRTQHG
                     NNTAYCQDNELSWFDWERADAELLEFTRRLAALRRRHPGFRRRGWFQGRPVRRGAGTA
                     LPDIAWFAPDGT"
BASE COUNT          444 a         1090 c         1049 g          419 t
ORIGIN      
        1 cgctcgctcg cggctgcggg ctccccccat gcaggcgcgg taagcccgcg tgcggcggcg
       61 accgtaccgt gtgccgccag cggtcaccgt ttcggcacga acatcgcaat gccgagcgga
      121 gatgccgatg tttccgtcag acgagctgag aacgtctgcg agtcaaccgc tcgacgacga
      181 ttctccggat ctcgactgga cgctggcgct cgcgggtggc gagggcgtcc ggctgtccga
      241 gtacgtccag cgcaggttcg gccgacggat ccccaagcag tactgctgcc tgctcggcga
      301 gcgctcgatg ctcgagcaca cgctggagcg gctcaacaag ctgaccccgc cgtcgcgcac
      361 gctcaccgtg atcggcaccg atcacggcga gatcgcggcg ccccagctcg ccggccgcag
      421 cgatcacgtg ttccgccagc ccagcgcgcg cgacaccggg gtcgcgctgt acgtcgcgct
      481 cgcgatgatc cggcgctggc acgccaacgc gaccgtcacg atcacgccga ccgaccacta
      541 cgtggtgccg tcggcgcgct acgtcgagca ggtccggatc gcgcgcgatg tcgcgcgcca
      601 gctgcgcgac gcggtggtgg tgctcggcgt ccggccgacc gagcccgatc cggagctcgg
      661 ctacctgacg ctcggcgacc ggctggccga gatccccgag ctccgccgcg tggtcggctt
      721 cgtcgagaag ccgacgccga cgcgcgccca tcagctgatc gccgagggcg ggctgtggaa
      781 cacgatggtc gcgtgcggca cggtcgacgc gctgtgggag ctcggccgcc agaccgagcc
      841 gcagctgatc gatatcctcg actcgttcgt gccgctggtc ggcacccgcg acgagaccga
      901 tgcgatcgag tacatctacc gcgcgatcct gccggtcagc ctgtcgcgcg acgtgttcga
      961 gcgcgcgccc gagcggctgg tcgtgcagca gctcgacggg gtcgactgga gcgactgggg
     1021 ccggccggag cggatcgagt cggtgctggc ggttcgccgg tcgcgcgcgc tggtcccgaa
     1081 cagggcgtac gcgccgtgac cgccgacgcg accgggcgcg cggcccccgc atccgacgcc
     1141 gcgaccggac ctgccgccga tccggcgagc gggcggcgcc ggcaggtgtg gccgggccgg
     1201 tcgtacccgc tgggcgcgac gttcgacggc tacggcacca acttcgccgt gttcagcggc
     1261 gtcgccgagc gggtcgagct gtgcctgttc gatcccgaag cggaccggcc cggaggcggc
     1321 cggcccgcgg aagctcgcat cgatctcaac cgcgggatcg gccagatccg gcacgcctac
     1381 ctgccgctgg ccggtccggg catgcgctac gggtttcgcg tccatggccc gtgggatccg
     1441 gcgcgcggta tgcgctgcaa cccgaacaag ctgctggtcg acccgtacgc ccgcgcgatc
     1501 tccgagggcc tgcactgggg gtccgcgctg cgcggcgacg acggcaacgg cggagcgtcg
     1561 accgaggaca gcgcgccgca cacgctgcgc tcggtggtcg tccagccgta cttcgactgg
     1621 ggcaccgatc gccggctcga gaccccgtgg aaccagacgg tgatctacga ggtccacgtc
     1681 aagggcctga cgatgcgcca ccccgacgtg ccgccgcacc tgcgcggcac ctacgccggg
     1741 ctcgctcacc ccgcgatcat caagtacctc cagagcctcg ggatcaccgc ggtcgagctg
     1801 ctgccgatcc acgcgttcat ccacgacggc ttcctgctcg agaaggggct gcgcaactac
     1861 tggggctacc actcgatcgg ctacttcgcg ccgcaccccg agtacgccag cgtgcacagc
     1921 gcgagcgggg cggtcgccga gttcaagcac atggtgcgga cgctgcacca tgccgggatc
     1981 gaggtgatcc tcgacgtggt ctacaaccac accggcgagg gcaaccacga cggcccgatg
     2041 ctgtcgttcc gcggcctcga caacatggcg tactaccggc tggtccccga ggacctgagc
     2101 tactacatgg actacacggg gacgggcaac acgctgcaga tgcgcagccc gcacaccctg
     2161 cagctggtga tggactcgct gcgctactgg atcgaggaga tgcacgtcga cgggttccgc
     2221 ttcgatctcg catcggcgct cgcccgcggc ctgcacgagg tcgaccggct cggcgcgttc
     2281 ttcgatctga tccagcagga tccggtggtg tcgcgggtca agctgatcgc cgagccgtgg
     2341 gacgtcggcg acggcggcta ccaggtcggc aacttcccgc cgctgtggtc ggagtggaac
     2401 ggcaagtacc gcgactggat ccgcgattac tggcgcggcg agcccggctc gctgcccgag
     2461 ctcggcgcgc ggatcaccgg cagctccgac ctctaccagc agggcgggcg gttcccgcac
     2521 gcgagcatca acttcgtgac cgcgcacgac ggcttcacgc tgcgcgatgt ggtctcgtac
     2581 aacgagaagc acaacgaggc caacggcgag ggcagccgcg acggcgagag ccacaaccgt
     2641 tcgtggaact gcggcgccga gggcgacacc gacgaccccg aggtggtcgc gctgcgccgc
     2701 cgccagcagc gcaacttcct cgcgaccctg ttcctgtcgc agggcgtgcc gatggtgctc
     2761 ggcggcgacg agctcggccg gacccagcac ggcaacaaca ccgcgtactg ccaggacaac
     2821 gagctgtcgt ggttcgactg ggagcgcgcc gacgccgagc tgctcgagtt cacccgccgg
     2881 ctcgccgcgc tgcgccgccg ccacccgggg tttcgccggc gcggctggtt ccagggccgg
     2941 ccggtccgcc gcggggccgg caccgcgctg cccgacatcg cgtggttcgc ccccgacggc
     3001 ac
//
LOCUS       DASVOR010001197         4415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_41462,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001197 DASVOR010000000
VERSION     DASVOR010001197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41462"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..612)
                     /locus_tag="VF516_22695"
                     /pseudo
     CDS             complement(1..612)
                     /locus_tag="VF516_22695"
                     /inference="COORDINATES: protein motif:HMM:NF012760.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
     gene            complement(679..1335)
                     /gene="rpe"
                     /locus_tag="VF516_22700"
     CDS             complement(679..1335)
                     /gene="rpe"
                     /locus_tag="VF516_22700"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF004076.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEX8110567.1"
                     /translation="MPLKIAPSILSADFGRLAEEVAAIASADYVHVDVMDGHFVPNLT
                     IGPVVVEAVRRATRLPLDVHLMIEDAERWVAAYAHAGADLIGVHVEACPHLHRTLHQI
                     RDLGKRPCVVLNPATPLESIEWVLGDVDQVLIMSVNPGFGGQQFIASALDKIRLLREM
                     IDERRLAVDIEVDGGVKLDNIAAVVAAGANVLVSGSGIFGTQDYAATIAAMRAHGAAR
                     "
     gene            complement(1408..1980)
                     /locus_tag="VF516_22705"
     CDS             complement(1408..1980)
                     /locus_tag="VF516_22705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110568.1"
                     /translation="MDDDQDIREYLQDFLNAEGFEVTTLADPSLAVERIRDEVFHLVV
                     LDLMMPKISGLDLLAQIRAVDDDIAVIILTGYPSLETASASIQHEVSAYIHKPFTPAD
                     FREVIARIAKKKGLVLRREDELHAAIGRQIRELRKTRSLTLKQMARRTNLSVSLLSQI
                     ERAESSASVSSLFKVATALDVRLTDLFAGY"
     gene            complement(2125..2198)
                     /locus_tag="VF516_22710"
     tRNA            complement(2125..2198)
                     /locus_tag="VF516_22710"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2162..2164),aa:Pro,seq:cgg)
     gene            complement(2236..2739)
                     /locus_tag="VF516_22715"
     CDS             complement(2236..2739)
                     /locus_tag="VF516_22715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110569.1"
                     /translation="MRAAAAEPVTAVTGPSSATPVVAPPVRISPTSDLDGTYLWLGPL
                     GAASRIDARWDSTFGADAAVVVVHERARLALVGGDLGASRWTARGGGRVWVDGLFGTY
                     LLGPLAGISLGPILELSDVTHPRLGGSVGVWGFAGITPFARVGSVANLGMFAEVGIHI
                     ALPVLRR"
     gene            2931..>4415
                     /locus_tag="VF516_22720"
     CDS             2931..>4415
                     /locus_tag="VF516_22720"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8110570.1"
                     /translation="MEDRNTQLPAPPFPGEMSNLAIQRSVPMGPGMPPPGMEADYGVI
                     RALPLFEGIPDDALIHAMSQGGISLRSLERDMFVLDPIGLAAGQVAPVIYVARGQVAA
                     AVFTETDLQERRAAQIAHENATAEEREEESLIKPAPLARIALKNVALFMEGDLFNSAA
                     LSATHGQPIAFYTTAPSALIALDQRWIAELAVRYPMFEQRLRRALSVSRERLAWVSGV
                     KQELLDFFVRQGVSVAGQVVRVRQLDLCIDCKLCEEACEERYGARRLTLGGYQLGMLD
                     FIYTCRTCTDQRCIDPCAYDSIRFDADKREVVINESTCTGCTACAQSCPYGAIDMIEV
                     EPEAPTFKQDFKARLEKKNALAFGPGSPRIARARRIANKCDHCMAYGDQACVSACPTG
                     SLIEVNPYNLFRERSPRMAQLARTGYDDDLARRDRREVLPVMPFTEGLAVRSGGLAKV
                     KRGRYAPLVIWVLGIFAFLLALGEVLLRLYAPTMSYQFATLRASP"
BASE COUNT          669 a         1588 c         1535 g          623 t
ORIGIN      
        1 cgcgccatcg gccttggcca gcatcggcgc gtgactcgcg gcggcgtggt cctgcgccac
       61 cgcgtgcgcg gtcccccccg ccatcgccgc gatcacctcg agcagcgcct cggcgccgac
      121 cggcgccagc cgcgcgagca gctcgccggc ggtctcgtcc ggatcgatcg ccacgcggcg
      181 ctcgaggtac accgggccgg tgtccatgcc ctcgtcgagc tgcatgatcg acaccccggt
      241 ctcggcgtcg ccgtggatca ccgcccactg gaccggcgcg gcgccgcggt agctcggcag
      301 cagcgagccg tggacgttga ggcagccgcg cggcagcgcg tcgagcaccg gcttcggcta
      361 gatcttgccg tacgcgacca ccaccgcgag ctccgcgccg gtcgcgacca gggcgtccgc
      421 gagctcgccg gtccgcgccg atcgcggctg gatcaccggc agcccgagct cctgcgccgc
      481 cgccttcacg gccggcgccg tcagctgccc gccgcgcccg gccggcttgt ccggctgcga
      541 cacgaccagc gcgacctcgt gctgcgcgtg cagcgcgcgc aggcacggca ccgcgaactc
      601 gggagatccc atgaagacca cgcgcacgcc gcggatctat cacccgccgc acacccgcgg
      661 tagcgcgcgg gccggcgatc agcgcgcggc gccgtgtgcg cgcatcgcgg cgatcgtcgc
      721 ggcgtagtcc tgggtgccga agatccccga gccgctgacg aggacgttgg cgcctgccgc
      781 gaccaccgcg gcgatgttgt cgagcttgac cccgccgtcg acctcgatgt cgaccgcgag
      841 gcggcgctcg tcgatcatct cgcgcagcag gcggatcttg tccagcgcgc tggcgatgaa
      901 ctgctggccg ccgaaccccg ggttgaccga catgatcagg acctggtcga cgtcgccgag
      961 cacccactcg atcgactcga gcggcgtggc cgggttgagc acgacgcacg gccgcttgcc
     1021 gaggtcgcgg atctggtgga gcgtgcggtg caggtgcggg caggcctcga cgtggacgcc
     1081 gatcaggtcg gcgccggcgt gggcgtacgc ggcgacccag cgctcggcgt cctcgatcat
     1141 gaggtggacg tcgagcggaa gccgggtcgc gcggcggacc gcctccacca cgaccgggcc
     1201 gatcgtgagg ttggggacga agtggccgtc catgacgtcg acgtggacgt agtccgcgct
     1261 ggcgatcgcg gcgacctcct cggcgagccg gccgaaatct gcggacagga tcgagggcgc
     1321 gatcttgagc ggcacggcgg cacgctagca cggcctcgcc gcgcggccgc gccagccggc
     1381 gcggggggca tggcgtgcgc cggcgcgtca gtacccggcg aacaggtcgg tcaaccggac
     1441 gtcgagcgcg gtggcgacct tgaacaggct cgacaccgac gcgctcgact cggcgcgctc
     1501 gatctgcgag agcagcgaca ccgacaggtt ggtccggcgc gccatctgct tgagcgtcag
     1561 gctccgggtc tttcgcagct cgcggatctg gcggccgatc gcggcgtgga gctcgtcctc
     1621 gcgccgcagc accaggccct tcttcttcgc gatccgcgcg atcacctcgc ggaagtcggc
     1681 cggcgtgaac ggcttgtgga tgtacgccga gacctcgtgc tggatcgagg ccgacgcggt
     1741 ctccagcgag ggatacccgg tcaggatgat cacggcgatg tcgtcatcga ccgcccggat
     1801 ctgcgccagc aggtccagcc ccgagatctt gggcatcatc aggtcgagca cgaccaggtg
     1861 aaacacttcg tctcggatcc gttccacggc cagcgaagga tcggccagcg tggtgacctc
     1921 gaacccttcc gcgttcagga aatcctgaag gtactcgcgg atgtcctggt catcatccac
     1981 gacgagcacc gacagcttcg aggccgtacg gaggggaact gcaccggcga cagcagcacc
     2041 tgcaaccatg ggggcgacca tggagccacg atatacgaaa atactgcgcc gcggagcccc
     2101 catgtggaac tccgcggcag gcagtcgggg cgacaggatt tgaacctgcg actccttggt
     2161 cccgaaccaa gtgcgctacc aggctgcgct acgccccgga aaggacacgt taggtagctt
     2221 gctcggagcg ttctgtcaac gccggaggac ggggagtgcg atgtgaatgc ctacctctgc
     2281 gaacatcccc agattcgcga cgctgcccac ccgcgcgaac ggcgtgatcc cggcgaaccc
     2341 ccagacgccg accgagccac cgaggcgcgg gtgcgtcacg tccgacagct cgaggatcgg
     2401 ccccagtgag attcccgcca gggggcctag caggtaggtg ccgaacagac catcgaccca
     2461 cacgcggcca cctccgcgcg cggtccaccg gctcgcgccg agatcgccac cgaccagagc
     2521 gagccgcgcg cgctcgtgaa cgacgacgac cgcggcatcg gcaccgaacg tcgagtccca
     2581 gcgcgcgtcg atccggctcg ccgcgccgag cgggccgagc cacaggtagg tgccgtcgag
     2641 gtccgaggtc ggcgaaatgc gcaccggcgg cgccacgacc ggcgtcgcgc tgctcgggcc
     2701 ggtgaccgcg gtcactggct cggccgccgc cgcgcgcatg acagcgagga aacacgcgat
     2761 ccagccgagg acagcacacc gaagccgccc cggcacagcc tgggggtgaa ccccccagtg
     2821 gaccggcttc agcgctatat aaaggggcca catcctgcta aattcgccgt ctacgctcgg
     2881 gggtgacggg ctcgtcgttt gcttctcgac cgtcctcgta cctcgtgagg atggaagacc
     2941 gcaatacgca gcttcccgcg cccccgttcc ccggcgagat gtcgaacttg gcgatccagc
     3001 gcagcgtccc catggggccg gggatgcctc cccccggcat ggaagcggac tacggcgtca
     3061 tccgggcgct accgctgttc gagggcatcc ccgatgacgc gctgatccat gcgatgtcgc
     3121 agggggggat ctccctgcgc tcgctggagc gcgacatgtt cgtcctcgac ccgatcggcc
     3181 tggccgccgg gcaggtcgcc ccggtgatct acgtcgcgcg cggccaggtc gccgctgcgg
     3241 tgttcaccga gaccgacctc caggagcgcc gggccgcgca gatcgcccac gagaacgcga
     3301 ccgcggagga gcgcgaggag gagtcgctga tcaagccggc gccgctggcg cggatcgcgc
     3361 tcaagaacgt cgcgctgttc atggagggcg acctgttcaa ctccgcggcg ctgtcggcga
     3421 cccacggcca gccgatcgcg ttctacacca cggcgccgtc cgcgctgatc gcgctcgacc
     3481 agcgctggat cgccgagctc gcggtgcgct acccgatgtt cgagcagcgg ctgcgccgcg
     3541 cgctgtcggt gtcgcgcgag cggctcgcct gggtgtccgg ggtcaagcag gagctgctcg
     3601 acttcttcgt ccgccagggc gtcagcgtcg ccggccaggt cgtccgggtc cgccagctcg
     3661 acctgtgcat cgactgcaag ctgtgcgagg aggcctgcga ggagcgctac ggcgcgcgcc
     3721 ggctgaccct gggcggctac cagctcggca tgctcgactt catctacacg tgccggacct
     3781 gcaccgacca gcggtgcatc gatccgtgcg cgtacgactc gatccggttc gacgccgaca
     3841 agcgcgaggt cgtgatcaac gagtcgacct gcaccggctg cacggcgtgc gcgcagtcct
     3901 gcccgtacgg cgcgatcgac atgatcgagg tcgagcccga ggcgccgacc ttcaagcagg
     3961 acttcaaggc ccggctcgag aagaagaacg cgctcgcgtt cggccccgga tcgccgcgga
     4021 tcgcgcgcgc gcgccggatc gccaacaagt gcgatcactg catggcgtac ggcgaccagg
     4081 cctgcgtgtc ggcgtgcccg accggctcgc tgatcgaggt caacccgtac aacctgttcc
     4141 gcgagcggtc gccgaggatg gcgcagctcg cgcgcaccgg ctacgacgac gacctggccc
     4201 ggcgcgatcg ccgcgaggtc ctgccggtga tgccgttcac cgaggggctc gcggtgcgct
     4261 cgggcgggct cgccaaggtc aagcgcggcc gctacgcgcc gctcgtgatc tgggtgctcg
     4321 ggatcttcgc gttcctgctc gcgctcggcg aggtcctgct ccggctgtac gcgccgacca
     4381 tgtcgtacca gttcgccacg ctgcgcgcgt cgccg
//
LOCUS       DASVOR010001198         4655 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_391610,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001198 DASVOR010000000
VERSION     DASVOR010001198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391610"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VF516_22725"
     CDS             <1..735
                     /locus_tag="VF516_22725"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8110571.1"
                     /translation="GKLARIEPAFGLGTIKREAGSRRIAVEASVSGRDLGSAARDVRD
                     ALARKLELPPGYFVDVGGKVESQTRAQQALYLAVGLALLAVFLLLYTALGSLAETVVI
                     LATLPSAFVGGIVALLIARESWSVSSLVGLIGLFGIAVQNGLVLVTQTRGLLRDGHAF
                     DEALREACIGRVRPKIMTAATAILGLLPLLVLRLHGTEIERPLAIVMIGGLVTSTAFT
                     LLALPTFYQLVHNLGVRWRARREVKP"
     gene            complement(746..1426)
                     /locus_tag="VF516_22730"
     CDS             complement(746..1426)
                     /locus_tag="VF516_22730"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX8110572.1"
                     /translation="MNRKQQLVGGKLAAQQHVKERRALALKVFQDVDDLVDREIARSV
                     AKGQPPTCTKGCSYCCRQETYAPRAEVEAIVEWLETSAPHLIPDLKTRIASWLDWYRT
                     EYPKLAASGIGRRDAFHSHGPPCPALVDDVCSIYPVRPVFCRTYYVTSPVDACRPQGD
                     PARPDVPIVSMRIFTKTADVATKLRVLIESQGADFNGTVHLLAEWLAHLLGVEAQPWR
                     TAAPVPVR"
     gene            1475..2884
                     /locus_tag="VF516_22735"
     CDS             1475..2884
                     /locus_tag="VF516_22735"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8110573.1"
                     /translation="MKVTLLGHASILVEMKAARCLMDPVFQDPFEDTAVVSCPRRAIH
                     LDKIPPIDLLVISHSHLDHFDIPSLAHVARQSRNCDVVCPKDHTIVYTLERLGFTKIH
                     PEEPHKHFKLRGYELLTTHSNVTNVTEIGIVFKDESGTFWNQVDTVISPATAAATPKQ
                     AGPIDLLFAMYASQNFDFFASRGTSFPHQMHAMNLNNVMAIRPRMTVPGSAGFRFAGT
                     GMEWCNAFLFPMSRERFVADLARLAPDLETRIANPGDVFEIDRGKVRHHVAASPIART
                     LEDDTHLLQFDPTAPIPPLQDPNPDGLSMQRLEAGVEDCVAGFLAFVREAYASGDPVV
                     DEYRKLRATYAVGVVFGDRREHWIHLAFSPEAPEITRCEDRCPPADAGHRIVASALTA
                     WAAREKSYFYLRAFSRQWSMLYTLAADGSRAVVEPAHPQDLLAYYLVRKAKGAELAIK
                     LWLDHQLAQYLPGRTGTAP"
     gene            complement(2904..>4655)
                     /gene="dauA"
                     /locus_tag="VF516_22740"
     CDS             complement(2904..>4655)
                     /gene="dauA"
                     /locus_tag="VF516_22740"
                     /inference="COORDINATES: protein motif:HMM:NF008660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HEX8110574.1"
                     /translation="AYRAKDLLGYGLRARLREGYSVKDFRADLLAALVVGVVALPLSM
                     ALAIAVDVPPQHGLYTAIIAGFTCALLGGTRFQVTGPTAAFVVILVPIVHRFGLSGLL
                     VAGMMAGVILIVMGLARLGKLMEFVPHPVTSGFTMGIAVVIGILQLRDALGVTLPKTE
                     GTFEYVAALWHARAHINPYDTGVAVVTIGLLLWLPRVLKRIPAPLVALTLVSLAVVLC
                     QHWWPGFHVSTIGNLEKFTIKIGNETIHGIPPLPPIPMLPWHGGEGIALDYHTIRELL
                     PSAFAIAMLGAIESLMAAVVADGMGGSRHDPNAELIALGVGNILCPFFGGIAATGALA
                     RTATNIRAGARSPFAAVMHSIFVLACTIVLAPLVAYLPMAALAGLLIIVARNMSEARH
                     FARLLRIAPRNDVLVMVTCFTLTVVFDMVIAVAVGVVLAALLFMRRMAVTTTVELETA
                     QELGLGIELPPGIRLYEVAGPMFFGAAKSAMEAIATVGSGGDHTFVLNMQHVPTIDVT
                     ALVALESVLDRLYRSRIKVIFAGLTPEVAELLDRAGIKRQPGRLAYAPDVETAISMAI
                     VHGARLPVSAQPPPAAA"
BASE COUNT          754 a         1646 c         1559 g          696 t
ORIGIN      
        1 ggcaagctgg ccaggatcga gccggcgttc gggctcggca ccatcaagcg cgaggccggc
       61 agccgccgga tcgccgtcga ggccagcgtg tccggacgcg acctgggctc ggccgcgcgc
      121 gatgtgcgcg atgcgctcgc caggaagctc gaactcccgc ccggctactt cgtcgacgtc
      181 ggcggcaagg tcgagagcca gacccgcgcg caacaggcgc tctacctcgc ggtcggtctg
      241 gcgctgctcg cggtgttcct cctgctctac accgcgctcg gctccctcgc cgagaccgtc
      301 gtgatcctcg cgaccttgcc cagcgcgttc gtcggtggga tcgtcgcgct cctgatcgcg
      361 cgcgagagct ggagcgtctc gtccctggtc gggctgatcg gcctgttcgg gatcgcggtg
      421 cagaacggcc tcgtgctcgt cacccagacg cgcggcctcc tccgcgatgg ccacgcgttc
      481 gacgaggcgc tgcgcgaagc gtgcatcgga cgcgtgcggc ccaagatcat gacggcggcg
      541 accgccatcc tgggcctcct gccgctgctc gtgctccggc tgcacggcac cgagatcgag
      601 cgaccgctgg cgatcgtgat gatcggcggc ctcgtgacat cgacggcgtt cacgttgctc
      661 gcgctgccga cgttctatca gctggtccac aacctgggcg tccggtggcg cgcacgccgg
      721 gaggtgaagc cgtgacccgc agccgtcacc ggaccgggac cggcgccgcg gtccgccacg
      781 gctgtgcctc cacgccgagc aggtgcgcca gccattcggc caggaggtgc acggtcccgt
      841 tgaagtcggc tccttgactc tcgatcagca cgcgtagctt cgtggcaaca tcggccgtct
      901 tggtgaagat ccgcatcgac acgatgggca catccggacg tgccggatcg ccctgagggc
      961 gacaggcatc taccggactg gtgacgtagt acgtccggca gaacaccggt cgcaccggat
     1021 agatcgagca cacgtcgtcg accagggcag gacacggagg tccgtgagag tggaacgcgt
     1081 cgcgccggcc gatgccgctc gctgcgagct tcgggtactc ggtccggtac cagtcgagcc
     1141 agctcgcgat cctggtcttg aggtcgggga tcaggtgagg ggcggacgtc tcgagccact
     1201 cgacgatggc ctcgacctcg gctcgcggcg cgtaggtttc ctgccggcaa cagtacgagc
     1261 accccttcgt acatgtcggt ggctgtccct tcgcgaccga cctggcgatc tcacggtcga
     1321 ccaggtcgtc gacgtcctgg aacaccttga gcgcaagcgc gcgccgctcc ttgacatgct
     1381 gctgcgctgc cagcttgccg ccgacgagtt gctgttttcg attcatgcgt cctcgcgagc
     1441 gtggcgatct gccccacctg taaactcatc cccgatgaaa gtcacgctcc tcggccacgc
     1501 atcgatcctc gtcgagatga aggcggcccg atgcctgatg gatccggtgt ttcaggatcc
     1561 cttcgaggac acggcggtcg tgtcttgccc ccggcgcgcg atccacctcg acaagatccc
     1621 cccgatcgac ctgctcgtga tctcgcacag ccatctcgac cacttcgaca tcccgtcgct
     1681 cgcccacgtc gcccggcagt cgcgcaactg cgacgtcgtg tgtccgaagg accacaccat
     1741 cgtctacacc ctggagcggc tcggcttcac gaagatccat cccgaggaac cgcacaagca
     1801 cttcaagctg cgcggctacg agctgctcac cacgcactcg aacgtcacca acgtcaccga
     1861 gatcggcatc gtcttcaagg acgagtccgg cacgttctgg aaccaggtcg acacggtgat
     1921 ctctccagcg accgccgccg cgacccccaa gcaggccggc cccatcgacc tgctgttcgc
     1981 gatgtatgcg agccagaact tcgacttctt tgcgagccgg gggaccagct tcccgcacca
     2041 gatgcacgcg atgaacctca acaacgtcat ggcgatccgg ccgcggatga ccgtgcccgg
     2101 atccgcgggc ttccgctttg ccggtacggg catggagtgg tgcaacgcat tcctgttccc
     2161 gatgtcgcgc gagcgcttcg tcgcggatct cgcccgcctg gcacccgacc tggagacccg
     2221 gatcgcgaat ccgggagacg tgttcgagat cgaccgcggc aaggtgaggc atcacgtcgc
     2281 cgcgtctccc atcgcgagga cgctggaaga cgacacccac ttgctccagt tcgatccgac
     2341 cgcgccgatc ccaccgcttc aggaccccaa ccccgacggt ctgtccatgc aacgactgga
     2401 ggccggcgtg gaggactgcg tggcgggctt cctcgcgttc gtccgcgaag cgtatgcgtc
     2461 cggcgatccg gtcgtcgacg agtaccggaa gctccgcgcg acctacgcgg tgggggtcgt
     2521 gttcggcgac cggagggagc actggatcca cctggcgttc tcccccgagg cacccgagat
     2581 cacgcgctgc gaggaccgct gcccgccggc cgatgccgga catcgcatcg tcgcctccgc
     2641 gctcaccgca tgggctgcgc gcgagaagag ctacttctac ctgcgtgcct tctcgcgcca
     2701 gtggagcatg ctctacacgc tggccgcgga tggatcgcgg gccgtggtcg agccggcgca
     2761 cccgcaggat ctgctcgcgt actacctggt gcgcaaggcc aagggggccg agctcgccat
     2821 caagctgtgg ctcgatcacc agctcgccca gtacctgccc ggacgcaccg gaaccgcgcc
     2881 gtagcaccac acgctgccac gctctaggcg gcggcgggcg gcggctgggc cgagaccggc
     2941 agccgcgcgc cgtggacgat cgccatgctg atcgcggtct cgacgtcggg cgcgtaggcc
     3001 agccggccgg gctggcgctt gatcccggcg cgatcgagca gctcggcgac ctcgggggtg
     3061 agcccggcga agatcacctt gatccgcgag cggtacaggc ggtcgagcac cgactccagc
     3121 gcgaccagcg cggtgacgtc gatcgtcggc acgtgctgca tgttgagcac gaacgtgtgg
     3181 tcgccgccgc tgccgaccgt ggcgatcgcc tccatcgccg acttcgcggc gccgaagaac
     3241 atcgggccgg cgacctcgta cagccggatc cccggcggca gctcgatgcc gaggccgagc
     3301 tcctgcgcgg tctcgagctc gaccgtcgtg gtcaccgcca tccggcgcat gaacagcagc
     3361 gcggcgagca cgacgccgac cgccaccgcg atcaccatgt cgaacaccac ggtgagcgtg
     3421 aagcaggtga ccatgaccag cacgtcgttg cgcggcgcga tccgcaacag gcgggcgaag
     3481 tggcgggcct ccgacatgtt gcgggcgacg atgatcaaaa gcccggcgag cgcggccatc
     3541 ggcaggtacg cgaccagcgg cgcgagcacg atcgtgcacg ccaggacgaa gatcgaatgc
     3601 atcaccgccg cgaacggcga gcgcgccccg gcgcggatgt tggtcgcggt gcgcgcgagc
     3661 gcgccggtcg ccgcgatccc gccgaagaac gggcacagga tgttgccgac gccgagcgcg
     3721 atcagctcgg cgttgggatc gtggcgcgag ccgcccatgc catcggcgac caccgccgcc
     3781 atcagcgact cgatcgcgcc gagcatcgcg atcgcgaacg ccgacggcag gagctcgcgg
     3841 atcgtgtggt agtcgagcgc gatcccctcg ccgccgtgcc acggcagcat cgggatcggc
     3901 ggtagcggcg ggatgccgtg gatggtctcg ttgccgatct tgatcgtgaa cttctcgagg
     3961 ttgccgatcg tcgacacgtg gaagccgggc caccagtgct ggcagagcac gaccgcgagc
     4021 gacacgaggg tcagcgcgac cagcggcgcc gggatgcgct tgagcacgcg cggcagccac
     4081 agcaagaggc cgatcgtcac caccgcgacc ccggtgtcgt acgggttgat gtgggcgcgc
     4141 gcgtgccaca gcgccgcgac gtactcgaac gtgccctcgg tcttgggcag cgtcacgccg
     4201 agcgcgtcgc gcagctggag gatgccgatc acgaccgcga tgcccatcgt gaaccccgag
     4261 gtcaccgggt gcggcacgaa ctccatcagc ttgcccagcc gggccagccc catgacgatc
     4321 aggatcacgc cggccatcat cccggcgacc agcaggccgg acagcccgaa gcggtggacg
     4381 atcgggacca ggatgaccac gaacgccgcg gtcggcccgg tgacctggaa ccgggtgccg
     4441 ccgagcagcg cgcaggtgaa cccggcgatg atcgcggtgt acagcccgtg ctgcggtggc
     4501 acgtcgaccg cgatcgccag cgccatcgac agcggcagcg cgaccacgcc gacgaccagg
     4561 gcggccagca ggtcggcgcg gaagtccttg accgaatagc cctcgcgcag ccgcgcgcgc
     4621 aggccgtagc cgagcaggtc cttggcgcga taggc
//
LOCUS       DASVOR010001199         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_274415,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001199 DASVOR010000000
VERSION     DASVOR010001199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274415"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VF516_22745"
     CDS             complement(<1..399)
                     /locus_tag="VF516_22745"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX8110575.1"
                     /translation="MIDAATVWQERRLGRTIVKTRRAIVDLDDVLPNDKQPRIGPKED
                     PELQRQIEANEGIFEPLLLEPHPTLGDKFRIIDGDRRWSNSKVLVDQLKKPQYRQLPA
                     EITDHPLDDEERLRVWIYIHRQRKEWDAKEK"
     gene            complement(392..1510)
                     /locus_tag="VF516_22750"
     CDS             complement(392..1510)
                     /locus_tag="VF516_22750"
                     /inference="COORDINATES: protein motif:HMM:NF021427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEX8110576.1"
                     /translation="MVLAAVKQQDACPWCGQRISHKQFLDIEARIRTEEKRRFAVAEA
                     AAQAKLAAEREVLETRHRADRTALELAVQQLKEQLEKAREDQAEFDRRLREAIEVAAQ
                     TERKKAEAEAARKHQAEREHERELIKADFDQKMLKQDAENQKAAAALRKQVIELQRRL
                     EDQAARKPEVVDIDVFEQLKSACPDDRVLRLPKGEAGSDILLEVRYKGETCGRIVLDS
                     RNRGNWQNAYATRLRTDMVEQKAEHAILSTLHFPRGAAEMCRYDDVLLVHPARVVELV
                     GILRQAMVRMHQTRLSHVERNEKKLRLYDHITSDLFRRKLTDTGKLTQQLLDLDVDEL
                     KEHNRVWKLRGGILKRLETVNSEVVGEINDIVDGRDDD"
     gene            complement(1855..>2548)
                     /locus_tag="VF516_22755"
     CDS             complement(1855..>2548)
                     /locus_tag="VF516_22755"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX8110577.1"
                     /translation="MAHEWKIPIGTRRADPLAMNDFNVLTFNGKAFPATAPLVAEVGD
                     RVRIRFANLGPMDHHPLHLHGHTFELVGTDGGTVPPSARFPETTVLVPTGTTRTIELV
                     AHAAGDWPLHCHMTHHAMNQMGHDAANLIGADTDGLGAALGRAVPGTMVMGASGMGEM
                     SRMKMEQPRNSIAMLGGDGPHGTIDMGGMFTLLKIRDRLTGDGDPGWYQPRTDVAGEA
                     TDDELARDGITP"
BASE COUNT          324 a          835 c          912 g          477 t
ORIGIN      
        1 cttctccttg gcgtcccact ccttgcgctg gcggtggatg tagatccaga cccgcagccg
       61 ctcctcgtcg tcgagcgggt ggtcggtgat ctcggccggc agctggcggt actgcggctt
      121 cttgagctgg tcgaccagca ccttggagtt ggaccatcgc cgatcgccgt cgatgatccg
      181 gaacttgtcg cccagcgtgg ggtggggctc gagcagcagc ggctcgaaga tgccctcgtt
      241 ggcctcgatc tggcgctgca gctcgggatc ctccttgggg ccgatccgcg gctgcttgtc
      301 gttgggcagg acatcgtcga gatcgacgat cgctcgccgg gtcttgacga tcgtgcggcc
      361 gagccgccgc tcctgccaga cggtggccgc gtcaatcatc gtcgcgtccg tcgacgatgt
      421 cgttgatctc gccgacgacc tcgctgttga cggtctccag ccgcttgagg atgccgccgc
      481 gcagcttcca cacccggttg tgctccttga gctcgtcgac gtcgaggtcg agcagctgct
      541 gggtgagctt gccggtgtcg gtgagcttgc ggcggaacag atcggacgtg atgtggtcgt
      601 acagccgcag cttcttctcg ttgcgctcga cgtggctgag ccgggtctgg tgcatccgca
      661 ccatcgcctg gcgcaggatg ccgaccagct cgacgacgcg cgccggatgg acgagcagga
      721 cgtcgtcata ccggcacatc tccgcggcgc cgcgcgggaa gtgcagcgtc gacaggatcg
      781 cgtgctcggc cttctgctcg accatgtcgg tgcgcagccg ggtcgcgtac gcgttctgcc
      841 agttgccccg gttgcgcgag tcgaggacga tccggccgca ggtctcgccc ttgtacctca
      901 cctcgagcag gatgtcgctg ccggcctcgc ccttgggcag gcgcagcacg cggtcgtccg
      961 gacacgcgga cttgagctgc tcgaacacat cgatgtcgac gacctccggc ttgcgcgcgg
     1021 cctggtcctc cagccggcgc tgcagctcga tgacctgctt gcgcagggcg gcggcggcct
     1081 tctggttctc ggcgtcctgc ttgagcatct tctgatcgaa gtcggccttg atcagctcgc
     1141 gctcgtgctc gcgctcggcc tggtgcttgc gcgcggcctc ggcctcggcc ttcttgcgct
     1201 cggtctgcgc ggcgacctcg atcgcctcgc gcagccgccg gtcgaactcg gcctggtcct
     1261 cgcgcgcctt ctccagctgc tccttgagct gctggacggc gagctcgagg gccgtgcggt
     1321 ccgcccggtg gcgggtctcc agcacctcgc gctcggcggc gagcttggcc tgcgccgcgg
     1381 cctcggccac cgcgaagcgg cgcttctcct ccgtgcgaat gcgtgcttcg atgtcgagga
     1441 actgcttgtg ggaaatccgc tgaccgcacc agggacacgc gtcctgctgc ttcaccgctg
     1501 cgagcaccat cggcctctcc aaggaaaaaa aaggacctgg taggactcta taaggtcctg
     1561 acgtcagatc aaggtctgtt ctgcagctct tcgtaaatgc ctgtgtaggc ttcactcccg
     1621 tcgatacggc gaatccggat tggcgctaac ccccggaaat gggattccgg attgagcagc
     1681 aggtccggat tgaggagatc tcctcagcca ccggcgtggg tccggagtgg cgcggtgcgc
     1741 ggtggcctca tgatggccga aagccgcgag gtgctgcacc gcctggggcc ggagggcgca
     1801 tgccgcgaac cgctcggcgc cgagctgtcc ggaagacgga cgacagggcc cgtcctacgg
     1861 ggtgatgccg tcgcgggcga gctcgtcgtc ggtggcctcg ccggcgacgt cggtgcgcgg
     1921 ctggtaccag cccgggtcgc cgtcgccggt gagccggtcg cggatcttga gcagcgtgaa
     1981 catgccgccc atgtcgatcg tgccgtgggg gccgtcgccg ccgagcatcg cgatcgagtt
     2041 gcgcggctgc tccatcttca tgcgcgacat ctcgcccatg ccgcttgccc ccatgaccat
     2101 ggtgccgggg accgcgcggc cgagcgcggc gccgaggccg tcggtgtcgg cgccgatcag
     2161 gttggcggcg tcgtggccca tctggttcat cgcgtggtgg gtcatgtggc agtgcagcgg
     2221 ccagtcgccg gcggcgtgag cgacgagctc gatcgtgcgg gtggtgccgg tgggtacgag
     2281 cacggtggtc tcggggaacc gggcggacgg cgggacggtg ccgccgtcgg tgccgaccag
     2341 ctcgaacgtg tggccgtgga ggtggagcgg gtggtggtcc atcgggccga ggttggcgaa
     2401 ccggatgcgg acccgatcgc cgacctcggc gaccagcggc gcggtggcgg ggaatgcctt
     2461 gccgttgaac gtcagcacgt tgaagtcgtt catcgcgagc gggtcggcgc gccgggtgcc
     2521 gatcgggatc ttccactcgt gggccatg
//
LOCUS       DASVOR010001200         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_246046,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001200 DASVOR010000000
VERSION     DASVOR010001200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246046"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1830)
                     /locus_tag="VF516_22760"
     CDS             complement(<1..1830)
                     /locus_tag="VF516_22760"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX8110578.1"
                     /translation="MNQFTDIAADARGPADAPVEPIHLGDFTATRRMVYLAIIAIVLG
                     VIATYVAFALLQLIGLFTNVFYYQRLEFAPASPAGNTLGAASVVVPIAGALIIGLMAR
                     YGSERIRGHGIPEALEAILTRGSRVEPRVALLKPLSSAISIGSGGPFGAEGPIIMTGG
                     AFGSLVAQLFKLTSAERKTLLVAGAAAGMSATFASPVAAILLAVELLLFEWKPRSFVP
                     VALASATAAAARTFVIGPGPLFPLPAHPAFPGTAALACCALAGLVAGGFSALLTGAVY
                     AFEDLFKRLPIHWMWWPAIGGLVIGLGGLLCPQALGVGYDVIGQVLHDSYTARAILVL
                     VVVKGLIWSVSLGSGTSGGVLAPLLMMGGALGGLEGLVFPDAGVGFWPLVSMGAILGG
                     TMRSPLTGVVFAVELTHDQNMLLPLVIAVTVAHAFTVLVLRRSILTEKVARRGFHVSR
                     EYAIDELEILFVRDVMTTDLAMLSARDTPAGALSRIAPEGHARHERSQHLYPVLDDDA
                     NLVGAAMYAEVHAWTTDPARRNQPLRALASADPPVVFAHETLRSAVGRMAERGLTALI
                     AVNPANPRHVVGKIALHDLLKARARHLEDEQRRERILPFEYLLP"
BASE COUNT          296 a          701 c          695 g          225 t
ORIGIN      
        1 cggcagcagg tactcgaacg gcaggatccg ctcccggcgc tgctcgtcct cgaggtggcg
       61 cgcccgggcc ttgagcaggt cgtgcagcgc gatcttgccg accacgtggc gcgggttggc
      121 cgggttgacc gcgatcagcg cggtcagccc gcgctccgcc atccggccga ccgcggagcg
      181 cagcgtctcg tgggcgaaca ccaccggcgg gtccgcgctg gcgagggcgc gcagcggctg
      241 gttgcgccgg gccgggtccg tggtccatgc gtggacctcg gcatacatcg ccgcgccgac
      301 caggttggcg tcatcgtcga gcaccggata gaggtgctgg ctgcgctcgt ggcgggcgtg
      361 gccctccggc gcgatccggg acagcgcgcc ggccggggtg tcgcgggccg acagcatcgc
      421 gaggtcggtg gtcatcacgt cgcgcacgaa caggatctcg agctcgtcga tcgcgtactc
      481 gcggctgacg tggaacccac ggcgcgcgac cttctcggtc aggatcgacc ggcgcaggac
      541 cagcacggtg aacgcgtgcg ccacggtcac cgcgatcacc agcggcagca gcatgttctg
      601 gtcgtgggtg agctcgaccg cgaacaccac gccggtcagc ggcgatcgca tcgtgccgcc
      661 gaggatcgcg cccatgctga ccagcggcca gaacccgaca ccggcgtcgg ggaacaccag
      721 gccctccagc ccgccgagcg caccgcccat catgagcagc ggggcgagca cgccgcccga
      781 ggtccccgat ccgagcgaca ccgaccagat caggcccttg accaccacca gcacgaggat
      841 cgcgcgcgcg gtgtagctgt cgtgcagcac ctggccgatc acgtcgtagc cgacgccgag
      901 cgcctgcggg cagagcaggc cgccgagccc gatcaccagg ccgccgatcg ccggccacca
      961 catccagtgg atcggcaggc gcttgaacag atcctcgaag gcatacaccg cgccggtcag
     1021 cagcgccgag aacccgcccg ccacgagccc ggccagcgcg cagcacgcca gcgccgcggt
     1081 cccggggaac gccgggtggg ccggcagcgg gaacagcggt ccgggcccga tcacgaacgt
     1141 gcgggcggcg gcggcggtcg cgctcgccag cgccaccggc acgaagctgc gcggcttcca
     1201 ttcgaacagc aggagctcga ccgcgagcag gatcgccgcg accggcgagg cgaacgtcgc
     1261 cgacatcccc gccgcggcgc cggcgaccag cagggtcttg cgctccgccg aggtcagctt
     1321 gaacagctgg gcgaccagcg agccgaacgc gccgccggtc atgatgatcg gcccctcggc
     1381 gccgaacggg ccgcccgagc cgatcgagat cgccgacgac agcggcttga gcagcgcgac
     1441 ccggggctcg acccggctgc cccgggtcag gatcgcctcg agcgcctcgg gaatcccgtg
     1501 gccgcggatc cgctccgagc cgtagcgcgc catcaggccg atgatcagcg cgccggcgat
     1561 cgggaccacc acgctcgccg cgcccagcgt gttgccggcg ggcgacgcgg gggcgaactc
     1621 cagccgctgg tagtagaaca cgttggtgaa cagcccgatc agctggagca gcgcgaacgc
     1681 gacgtaggtc gcgatcaccc cgagcacgat cgcgatgatc gcgaggtaca ccatgcggcg
     1741 cgtcgcggtg aaatctccca ggtggatggg ttcgaccggc gcgtcggccg ggccgcgggc
     1801 gtccgcggcg atgtctgtga actggttcac ctggtccgtc ctcggcccga cctcgggccc
     1861 atcgtggggc tgccgccgcg gcgcttgccg gcgggcggct cgaagaacat cggcgcg
//
LOCUS       DASVOR010001201         5637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_450381,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001201 DASVOR010000000
VERSION     DASVOR010001201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450381"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VF516_22765"
     CDS             <1..294
                     /locus_tag="VF516_22765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110579.1"
                     /translation="GELSHAVRKRPRFTGDHEQVDGPVAIAGDERAVAEAALAPVADR
                     ILYGRVDVARDAAGPPMVMELELIEPSLYLTHHPPALDRFVAAIRRWRAELRA"
     gene            complement(314..3508)
                     /locus_tag="VF516_22770"
     CDS             complement(314..3508)
                     /locus_tag="VF516_22770"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NACHT domain-containing protein"
                     /protein_id="HEX8110580.1"
                     /translation="MLAALTGAASPRTRAQIEADIRQEADNDAAAGQRDAATALRVFA
                     DEASQVGMPPREIMTIYQDEYRQEIDKRKPGPWEAYRFQIMTVLAIVGFLGAIFRKSI
                     EDVLGKGLGRLWGAIYDRYAGSRLLRRWALTQYRRRLIDRLALLKLPFRPERPLAMRD
                     VYVPLRIAESQDAADRTRQVQEASDALANHRRLVVLGAPGSGKSMLVKHLALIGAERR
                     LPAALGEPIPVVVELHRFADPKATIESEIVAELGRTGFPHAETFVRQNLEKRSFLLLL
                     DGFDEIPAAQRSQIVQQIKDLVSKYDELRFVLTCRVAVYKGELYDVADRTLELIDFND
                     HQIERYLRTWTSSMPPGKSVDELITMLANRPQIKKLAGNPLLLTIIAYLYADHPDVVL
                     PHSRSQFYKTATDQLLERWHQDQNKFTLPQKRAILEHLALFNQAGADPTDADRRTMDF
                     RVVSEQVKAICPSLNLKPEDSDRVIQEIVERSGLLLAIDGGQRYCFAHLTLQEYFTAA
                     RLQHQPEQMIRHYQTDRDAWRETLRLWCGLAPDSTDVIHQVSAVDPVTAFECLADAQM
                     VSPELADAIIAELKGKLGTAGTEGAAIQAAFGTVAADPRVRGSEVLAFLIASLDDPEL
                     VPMAANALAQTGLMRAADALGTRVRSYTTAIRQALVTMSDVSVPILTRALDHGARWAL
                     EDLCAIGTPRVAEALVPLLWSATPGVAHRVALALGALLHNPHAEAALRGVALAPEHRA
                     STARVDWAWKPFHEPDGSSLPIIAGRIVFLIDAASSELLPRATLDKRIVLPACILQFA
                     REPGCVERIWKEDTSPPKTADRVDAWLDKLGASGKLRAVLAMMEPDLLARLIGKLVAK
                     SRAPTRDDWLSMFRPTSYDFQHGWHLRVYIAICLSLCVPAFLEVAQRLWSLPFELTRA
                     LAAALLAVMIVLGVLRAAFRWPTGEGSVSAVAILTFCGPIIMFGQMWDLARSNRHGDW
                     GVSVVGSVIGLLTYIWVLLAWSYGGDLLTRHLPPGVFAAWFLVAGACCALLILRIVKL
                     DYVAENPLFDLWDHVARPSATIRTIVTHRASSRS"
     gene            complement(3888..4463)
                     /locus_tag="VF516_22775"
     CDS             complement(3888..4463)
                     /locus_tag="VF516_22775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110581.1"
                     /translation="MKQGSGVMMALVVALSGTARADEAAPPPDRAKPFLTEDDSHVVT
                     VTGAFGPARDTMMVFAYHGLEGMGRFVGFALVSDDKAKHGQRKIELPALPQGSDSGEV
                     AVTLVANLDRDADDEIVVGFRVFRTVRGRQGGFSYNTWEYAVLDWNGKKFVRLPALEK
                     KLQARADAREEHKGSVLSEDDARAALGVAKK"
     gene            complement(4517..>5637)
                     /locus_tag="VF516_22780"
     CDS             complement(4517..>5637)
                     /locus_tag="VF516_22780"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8110582.1"
                     /translation="RLPQPVTGGAVLVTAPPGDDRLFVVEQRGAIRIYRGEVLAPQPF
                     LDLSAAAGGPVVAGGELGLLGLAFHPDYAHNRALFVFYTAHKLGDPAHPYMDVVARYQ
                     ASAADPDRADPTGHVVLAIDDFAATHNGGMIEFGPDGYLYIGTGDGGGEGDPARTGQD
                     PSARLGKLLRIDVDHPAPGLAYGIPPDNPFATGGGAPEVFALGLRNPWRWSFDRATGD
                     LWIGDVGEAQVEEVDVLLAGSQRGANLGWSMFEGASCCMTQPDHCRQQAPQQPCDPAG
                     QRFALDQRSHADGWQAIIGGQVYRGTCYPDLAGWYFYTDNVRGGLAKARLTALGTLEV
                     VDLPGAFPASPSSLHADARGELYETDTAGNVYHLEAGP"
BASE COUNT          879 a         1969 c         1956 g          833 t
ORIGIN      
        1 ggcgagctgt cgcacgccgt gcgcaagcgc ccgcggttca ccggcgacca cgagcaggtc
       61 gatggcccgg tcgcgatcgc cggcgacgag cgcgcggtcg ccgaggcggc gctcgccccg
      121 gtcgccgacc gcatcctgta cggccgggtc gacgtcgcgc gcgacgccgc cggcccgccg
      181 atggtgatgg agctcgagct gatcgagccg tcgctgtatc tcacccacca cccgccggcg
      241 ctcgaccgct tcgtcgccgc gatccgccgc tggcgggccg agctgcgggc gtgagcccac
      301 aggacgcagc agctcacgac cgcgacgagg cgcggtgggt cacgatcgtg cggatcgtgg
      361 cgctcggacg cgcaacgtgg tcccagagat cgaacagtgg attctccgcg acatagtcca
      421 gcttgacgat tcgcaatatg agcagcgcac agcacgcgcc cgcaaccagg aaccaggcgg
      481 cgaagacacc aggcgggagg tggcgcgtga gcagatcacc gccgtagctc caggccagca
      541 atacccagat gtacgtcaac agccctatga ccgagccaac tacggacacg ccccagtcac
      601 catggcgatt ggatcgggca agatcccaca tttgcccaaa catgataatc ggcccacaga
      661 acgtgaggat cgcgaccgca gacacgctgc cctcaccggt cggccatcgg aacgcagctc
      721 gtagcacgcc gagcacgatc atgaccgcga gcagcgcagc agcgagcgct cgggtgagct
      781 cgaatggcaa ggaccagagc cgctgggcga cctcgaggaa tgccggcacg cacagcgaca
      841 ggcagatggc gatgtacacg cgaaggtgcc atccgtgttg gaagtcgtag ctcgtggggc
      901 gaaacatcga gagccagtca tcgcgtgtcg gcgctctgga cttggcgacc agcttgccga
      961 tcaacctcgc taggaggtct ggctccatca tcgcgaggac cgcgcgcagc ttgccgcttg
     1021 caccgagctt gtccagccat gcatcgacac gatccgcggt tttgggtggg ctggtgtcct
     1081 ccttccagat ccgctccacg catcccggtt cgcgagcgaa ctgcaggatg cacgcgggca
     1141 gcacgatgcg cttgtcgagg gttgcgcgcg gcagcagctc gctggaggcc gcgtcgatca
     1201 ggaacacgat ccgtccggca atgatcggca gcgacgagcc gtcgggctcg tggaacggtt
     1261 tccacgccca gtccacgcgg gcggtcgacg cgcgatgctc gggcgcgagc gcgaccccgc
     1321 gcagcgcggc ctcggcgtgc gggttgtgga gcagtgcgcc gagcgccagc gcgacccggt
     1381 gcgcgacccc gggcgtcgca gaccacagca gcgggaccag cgcctcggcg acccgagggg
     1441 tcccgatcgc acacaggtcc tcgagcgccc atcgcgcacc atggtcgaga gcgcgggtga
     1501 ggatgggcac ggagacgtcg ctcatcgtga ccagcgcttg acggatcgcg gtcgtgtacg
     1561 agcgcacccg ggttcccagc gcatcggcgg cgcgcatcag gccggtctgc gccagcgcgt
     1621 tcgcggccat cggcacgagc tccggatcgt ccaggctcgc gatcaagaat gccagaacct
     1681 cgctgccacg aacccgcgga tcagccgcga ccgtgccgaa cgcggcctgg atcgcggcgc
     1741 cctcggtgcc ggcggtgcca agcttcccct tgagctcggc gatgatcgca tcggcgagct
     1801 cgggcgatac catctgcgcg tctgccaggc actcgaacgc cgtcaccgga tcgaccgcgc
     1861 ttacctggtg gatcacgtcg gtgctgtcgg gcgcgaggcc gcaccacagc cgcagcgtct
     1921 cgcgccaggc atcgcggtcc gtctggtagt ggcgaatcat ctgctcgggc tggtgctgca
     1981 gccgggccgc cgtgaagtac tcctgcagcg tgaggtgagc gaagcagtag cgctggccgc
     2041 cgtcgatcgc gagcagcagg ccgctgcgct cgacgatctc ctggatcacg cgatcgctgt
     2101 cctcgggctt gaggttgagg ctgggacaga tcgccttcac ctgctcggac acgacccgga
     2161 agtccatcgt ccggcgatcg gcgtcggtgg gatcggcgcc ggcctggttg aacagcgcga
     2221 ggtgctcgag gatcgcacgc ttctgcggca gcgtgaactt gttctgatcc tggtgccagc
     2281 gctcgagcag ctggtcggtg gcggtcttgt agaactgcga ccgcgagtgc ggcagcacga
     2341 catcggggtg atcggcatag agataagcaa tgatcgtcaa aagcagcgga ttgccggcca
     2401 gcttcttgat ctgcggacgg ttcgcgagca tcgtgatcag ctcgtcgacc gacttgccgg
     2461 gcggcatgct cgacgtccag gtgcgcaggt agcgctcgat ctggtgatcg ttgaagtcga
     2521 tgagctccag ggtccgatcg gccacgtcgt acagctcgcc cttgtagacc gcgacgcggc
     2581 aggtgaggac gaagcggagc tcgtcgtact tgctgaccag gtccttgatc tgctggacga
     2641 tttgcgaccg ctgcgccgcc gggatctcgt cgaagccgtc gagcagcagc aggaacgagc
     2701 gtttctccag gttctggcgg acaaaagtct cggcgtgcgg aaacccggtc cggcccagct
     2761 cggcgacgat ctcggactcg atcgttgcct tgggatcggc gaacctgtgc aactcgacca
     2821 cgacggggat cggctcgccg agcgccgccg gcaggcgccg ctccgcgccg atcagcgcga
     2881 ggtgcttgac cagcatcgac ttgccggatc ccggcgcgcc gagcacgacc aacctgcgat
     2941 ggttcgcgag cgcgtcggac gcctcctgca cctggcgggt gcggtccgca gcgtcctgcg
     3001 actcggcgat ccggagcggc acgtagacgt cgcgcatcgc gagcggccgc tcgggccgga
     3061 acggcagctt gagcagtgcg agccggtcga tcagccgccg ccggtactgc gtgagggccc
     3121 accggcgcag tagccggctg ccggcgtagc ggtcgtagat cgcgccccac aaccgcccga
     3181 ggcctttccc gagcacgtcc tcgatcgact tgcggaagat cgcgcccagg aacccgacga
     3241 tcgcgaggac cgtcatgatc tggaaccggt acgcctccca gggcccgggc ttgcgcttgt
     3301 cgatctcctg ccgatactcg tcctggtaga tcgtcatgat ctcgcgcggc ggcatgccga
     3361 cctggctcgc ctcgtcggcg aacacccgca gcgccgtggc ggcgtcgcgc tgacccgcgg
     3421 ccgcgtcatt gtcggcttcc tggcgaatgt cggcctcgat ctgcgcacgg gtccgcggcg
     3481 atgccgcccc ggtcagcgca gcgagcaacg cgatcgcctg gagccatcgg cgtacgacgc
     3541 gccgtcgtct tacccaaccc atcgatcacc tccggagagc gccctacgat aacagagggc
     3601 gggccgcagc gggcgccgga cgcgatgcac aggaccaggt cacgggccac cggaggcctg
     3661 ttgccaccga gttcggtctc gcgaagctcg tcgcgcgtcg gcgggaaggg ccgacggcag
     3721 cgacggaaac acgcccctgc aggtcgccga ccgcatccgg taccgtccgg cgtcgcgcgc
     3781 gatgcagccg gcccgccgat ggtgatggag gtcgagcagc tggagccatc gccgcagctc
     3841 acgcgctgtc cggcgccctc gtccgctccg ccgcggcgat ccgccgccta cttctttgcg
     3901 actccgagcg ccgcgcgggc gtcgtcctcg ctcaacacgc ttcccttgtg ctcttcgcgc
     3961 gcgtccgccc tggcctggag cttcttctcg agcgcgggca gacgcacgaa cttcttgccg
     4021 ttccagtcga gcaccgcgta ctcccaggtg ttgtacgaga acccaccctg ccggccgcgg
     4081 accgtccgga acacccggaa cccgaccacg atctcgtcat cggcgtcccg gtcgaggttc
     4141 gcgaccagcg tcaccgcgac ctctcccgaa tcactgccct gcggcagcgc gggaagctcg
     4201 atcttgcgct ggccatgctt cgccttgtcg tcggacacga gcgcgaaccc gacgaacctc
     4261 cccatgccct ccaggccgtg ataggcgaac accatcatcg tgtccctggc cggtccgaac
     4321 gcgccggtga cggtgacgac gtggctgtcg tcctcggtga ggaacggctt cgcacggtcc
     4381 ggaggcgggg ctgcctcgtc tgcgcgcgcg gtcccggaca gcgcgaccac cagcgccatc
     4441 atcaccccgg agccttgctt catcgccgta gtacgcgtga agccctcgcc agattccagg
     4501 gcggccgcgc tcgagatcac gggccggcct cgaggtggta cacgttgccg gcggtgtcgg
     4561 tctcgtagag ctcgccgcgc gcgtcggcgt gaaggctcga cggcgacgcc gggaacgcgc
     4621 cgggcaggtc gacgacctcc agcgtcccga gcgcggtgag ccgggccttc gccagcccgc
     4681 cgcgcacgtt gtcggtgtag aagtaccagc cggcgaggtc ggggtagcac gtgccgcggt
     4741 agacctgccc gccgatgatc gcctgccagc catcggcgtg gctgcgctgg tcgagcgcga
     4801 acctctggcc cgccggatcg cacggctgct gcggggcctg ctggcggcag tgatcgggct
     4861 gggtcatgca gcagctggcg ccctcgaaca tgctccagcc gaggttggcg ccgcgctggc
     4921 tgccggcgag cagcacgtcg acctcctcga cctgcgcctc gccgacgtcg ccgatccaca
     4981 gatcgccggt cgcgcggtcg aacgaccagc gccacgggtt gcgcaggccg agcgcgaaca
     5041 cctccggcgc gccgccgccg gtggcgaacg ggttgtcggg cgggatgcca taggcgaggc
     5101 ccggcgccgg gtgatcgacg tcgatccgca gcagcttgcc gagccgcgcc gatgggtcct
     5161 ggccggtgcg cgccggatcg ccctccccgc cgccgtcgcc ggtgccgatg tacaggtagc
     5221 cgtcgggccc gaactcgatc atcccgccgt tgtgagtcgc cgcgaagtcg tcgatcgcca
     5281 gcacgacgtg gccggtcggg tcggcgcgat cggggtcggc ggcgctcgcc tggtagcgcg
     5341 cgacgacatc catgtagggg tgagccggat cgccgagctt gtgggcggtg tagaacacga
     5401 acagcgcgcg gttgtgcgcg tagtcggggt ggaacgccag gccgagcagc ccgagctcgc
     5461 cgccggcgac caccgggcca ccggccgcgg ccgacaggtc gaggaacggc tgcggcgcga
     5521 gcacctcgcc gcggtagatc cggatcgcgc cgcgctgctc gacgacgaac agccgatcgt
     5581 cgccgggcgg cgcggtgacc aggacggcac cgccggtcac cggctggggc aaccggc
//
LOCUS       DASVOR010001202         2328 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_508803,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001202 DASVOR010000000
VERSION     DASVOR010001202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2328
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508803"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF516_22785"
     CDS             complement(<1..228)
                     /locus_tag="VF516_22785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110583.1"
                     /translation="MPAEGNGPRPSIFEQLRRRLRGVDHAQAPALPLGEALHPAALAA
                     VAVLVVNDWVYKPLAPGAITGKLSDLAGLAFA"
     gene            247..1623
                     /locus_tag="VF516_22790"
     CDS             247..1623
                     /locus_tag="VF516_22790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110584.1"
                     /translation="MSESGKQSESAKQLAAAELQAQQRFERAMTELREGAYRFSSRAR
                     GQSELIIEELIDPEGETVASIAEVEGDQGAVALAQSLELITFDTWLFGQIGDKDQLDL
                     KAEEHWEVWFSFGAWIGETIRRRHGGHWLLLGDDPHTWRVGFSKIFLEIAPFVFGEQL
                     LRMGSGATRKMVSEIERIRQLHVEQTERDKGQSLDRFVAQHYIRLHTVPLGQWMAMDF
                     THLAKMWNEEPVAKLIVDIKEKGPRLGPQNSGIIDQVVGALARAKQDDPVAKQTTDRG
                     LFEAVAQIVGLRRATAPVAIDILERVVMPALHVGIPEKFPPLDEDDLANLRKGIELFA
                     FFVEVVPHKFQADDEGFLGTIPHDQLTTPYADRNTLEIGKGDWVVVNPAHFLPMIRDL
                     DPQKLLEKYDEFVKYLATIPDAPRRRDDGRMLAETAVRALADLKQCVGTAAQNKDALL
                     FRLLPPPG"
     gene            1620..1889
                     /locus_tag="VF516_22795"
     CDS             1620..1889
                     /locus_tag="VF516_22795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110585.1"
                     /translation="MSCALWITTSRQNRAIAGSRWQMPVARCATQLGVFVEQVNTGAA
                     ARQALTACWLSAVVPAPLSRLQVRPAAAVLVMLLIFLFTVFALFH"
     gene            complement(1883..2266)
                     /locus_tag="VF516_22800"
     CDS             complement(1883..2266)
                     /locus_tag="VF516_22800"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX8110586.1"
                     /translation="MTETGPRVTADDVRRYERELGHELPEDYRQFLVDVNGGQPSLER
                     SVFHLRKDRSILNNLLSLNDPDEGNDLATAQRLSGYSRNDLPREALTIGYDAGGSRIL
                     LILAGPHRTTHREDRGSAWHRRDGQ"
BASE COUNT          372 a          791 c          795 g          370 t
ORIGIN      
        1 cgcaaacgcc agcccggcga ggtcgctgag cttgccggtg atcgcgcccg gcgcgagcgg
       61 cttgtacacc cagtcgttca cgacgagcac cgccaccgcg gccagcgcgg ctggatgcag
      121 cgcctcgccg agcggcagcg ccggcgcctg ggcgtgatcg acgccgcgca gcctgcggcg
      181 cagctgctcg aagatcgagg gacgcgggcc attgccctcg gccggcacct gtataccatg
      241 ccggtcgtgt cggagtccgg caagcagtcc gagagcgcca agcaactcgc cgccgccgag
      301 ttgcaggcgc agcagcggtt cgagcgtgcg atgaccgagc tgcgcgaagg cgcctaccgg
      361 ttcagctcgc gcgcgcgcgg ccagtccgag ctgatcatcg aggagctgat cgatcccgag
      421 ggcgagaccg tcgcgtcgat cgccgaggtc gagggcgacc agggagcggt cgcgctcgcc
      481 cagtcgctcg agctgatcac gttcgatacc tggctgttcg gccagatcgg cgacaaggac
      541 cagctcgacc tcaaggccga ggagcactgg gaggtgtggt tctcgttcgg cgcgtggatc
      601 ggcgagacca tccgccggcg ccacggcggg cactggctgc tgctcggcga cgaccctcac
      661 acctggcgcg tggggttctc caagatcttc ctggagatcg cgccgttcgt gttcggcgag
      721 cagctgcttc gcatgggctc gggcgccacc cgcaagatgg tgagcgagat cgagcggatc
      781 cggcagctcc acgtcgagca gaccgagcgc gacaagggcc agtcgctcga ccgcttcgtc
      841 gcccagcact acatccggct gcacacggtg ccgctcggcc agtggatggc gatggacttc
      901 acccacctgg cgaagatgtg gaacgaggag ccggtcgcca agctgatcgt cgacatcaag
      961 gagaagggcc cccggctcgg gccccagaac agcgggatca tcgatcaggt ggtcggcgcg
     1021 ctcgcccgcg ccaagcagga cgatccggtc gccaagcaga ccacggatcg cgggctgttc
     1081 gaggcggtcg cccagatcgt cgggctgcgc cgcgccaccg cgccggtcgc gatcgacatc
     1141 ctggagcgcg tcgtgatgcc ggcgctccac gtcgggatcc ccgagaagtt cccgccgctc
     1201 gacgaggatg acctcgccaa cctgcgcaag ggcatcgagc tgttcgcgtt cttcgtcgag
     1261 gtcgtaccgc acaagttcca ggccgacgac gagggcttcc tcggcacgat cccgcacgac
     1321 cagctgacca cgccgtacgc cgaccgcaac acgctcgaga tcggcaaggg cgactgggtg
     1381 gtcgtcaacc cggcgcactt cctgccgatg atccgcgacc tcgatccgca aaagctgctc
     1441 gagaagtacg acgagttcgt caagtacctg gcgaccatcc ccgacgcccc gcgccgccgc
     1501 gacgacggcc ggatgctcgc cgagaccgcc gtccgcgcgc tcgccgacct caagcagtgc
     1561 gtcgggaccg ccgcgcagaa caaggacgcg ctgctgttcc ggctgctgcc gccgccaggg
     1621 tgagctgcgc cctgtggatc accacttcgc gacagaaccg cgccatcgcc gggagtagat
     1681 ggcagatgcc agtggccaga tgcgcgacgc aattaggggt attcgtcgag caggtcaaca
     1741 ccggcgcggc tgctcggcag gccctgacgg cttgctggct ttctgcggtg gtgccggcgc
     1801 cgctgtcccg gctacaggtg cgtccggcag cggccgtgct cgtcatgctg ctgatattcc
     1861 tgttcacagt tttcgcgctg tttcactgac catctcgacg atgccacgct gagccgcgat
     1921 cctcgcgatg ggtggtacgg tgtggccccg cgaggatcag caggatgcgc gagccgccgg
     1981 catcgtagcc gatcgtgagc gcttccctcg gaaggtcgtt ccttgagtac ccggagagcc
     2041 gctgggcggt cgccaggtcg tttccctcgt cggggtcgtt caggctcagc aggttgttga
     2101 ggatgctgcg gtccttgcgc aggtggaaca ccgagcgctc gagagatggt tggccgccgt
     2161 tcacgtcgac gaggaactgc cggtagtcct cagggagctc gtggccgagc tcgcgttcat
     2221 agcggcgaac gtcgtccgcg gtcacgcgcg gacccgtctc ggtcatcggg atccagctag
     2281 tcgtcatacc cgatgcctcc ggtgctgtcc ctgtaccggg cctagtta
//
LOCUS       DASVOR010001203         1502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_508807,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001203 DASVOR010000000
VERSION     DASVOR010001203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508807"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF516_22805"
     CDS             complement(<1..458)
                     /locus_tag="VF516_22805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110587.1"
                     /translation="MVELYQARRGFDARAVYDVWIRLVQSEPLYEAMLRGEHAAVARA
                     LGFTELELAILDDFAIQPGTRWHVENLRFRAVTMVSRLLGWHLPATLRLVTEGRDGWL
                     RDLVYEYLSLHRWVEFGHHHRFAECERFAALVEHRVLQRRRPPPHIAAVLG"
     gene            complement(451..>1502)
                     /locus_tag="VF516_22810"
     CDS             complement(451..>1502)
                     /locus_tag="VF516_22810"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEX8110588.1"
                     /translation="RRAAPRSAARGEAASGERRTERSCRAGYTGAAPGGSDRPLPWLR
                     RAARWFIVRSMQSAVGGVGFQINFFTRPLLDELERAGARIDYLEILCDTVSGSLEGPH
                     LVEPRHAGWLADLQRRYPLVAHSNFGEEYGFAELDDTPCVRRHVPIARAMGAAWMTDH
                     MFYGTQSTSYLWSTPIQFSAAEIRRVAARARRLQELLGMPLLHENAFIYALFPGSDLG
                     EAEFLSGLVEQAGTHLLLDVHNVYANSINFAGLDPWQYIDRIPLDRVLEIHVAGGQWI
                     DGWYHDLHNDCVPEPVWELVQHVLERSRSVRGVTLEVQGPVHTAQSRPIDAGWRDMAL
                     RDLERIAALWSRTRG"
BASE COUNT          228 a          541 c          543 g          190 t
ORIGIN      
        1 ccgagcacgg ccgcgatgtg cggcggcggc cgccggcgct ggagcacgcg gtgctcgacg
       61 agggccgcga accgctcgca ctcggcgaac cggtggtggt gcccgaactc gacccagcgg
      121 tgcagcgaca ggtactcgta gaccagatcg cgcagccagc cgtcgcggcc ctcggtcacc
      181 aggcgcagcg tcgccggcag gtgccagccg agcagccgcg acaccatggt caccgcgcgg
      241 aaccgcaggt tctcgacgtg ccaccgcgtg ccgggctgga tcgcgaagtc gtcgaggatc
      301 gcgagctcga gctcggtgaa gccgagcgcg cgggcgaccg ccgcgtgctc gccgcgcagc
      361 atcgcctcgt agagcggctc gctctgcacc agccggatcc acacgtcgta gaccgcgcgg
      421 gcgtcgaacc cgcggcgcgc ctggtacagc tcaaccacgg gtgcggctcc acagcgccgc
      481 gatgcgctcg aggtcgcgca gcgccatgtc gcgccacccg gcgtcgatcg gccgcgactg
      541 cgcggtgtgc accggcccct ggacctccag cgtcacgccg cgcaccgacc ggctgcgctc
      601 cagcacgtgc tggaccagct cccacaccgg ctcggggacg cagtcgttgt ggagatcgtg
      661 gtaccagccg tcgatccact ggccgccggc gacgtggatc tccagcaccc ggtcgagcgg
      721 gatccggtcg atgtactgcc acggatcgag gcccgcgaag ttgatcgagt tggcgtagac
      781 gttgtggacg tcgagcagga ggtgcgtgcc ggcctgctcg accaggccgg acaagaactc
      841 ggcctcgccg agatccgagc cgggaaacag cgcgtagatg aacgcgttct cgtggagcag
      901 cggcatgccg agcagctcct gcagccggcg cgcgcgggcg gcgacccgcc ggatctcggc
      961 cgccgagaac tggatcggcg tcgaccacag gtacgaggtc gactgcgtgc cgtagaacat
     1021 gtgatcggtc atccacgccg cgcccatcgc gcgcgcgatc gggacgtggc ggcgcacgca
     1081 cggcgtgtcg tcgagctcgg cgaagccgta ctcctcgccg aagttgctgt gcgcgaccag
     1141 cggatagcgg cgctgcaaat ccgccagcca cccggcgtgg cgcggctcga cgaggtgcgg
     1201 gccctccagc gagcccgaga ccgtgtcgca caggatctcc aggtagtcga tccgggcgcc
     1261 ggcgcgctcg agctcgtcga gcagcgggcg cgtgaagaag ttgatctgga agccgacgcc
     1321 gcccactgcg gactgcatgg accggacgat gaaccagcgc gcggcgcggc gcaaccacgg
     1381 cagcgggcga tcgctgccgc cgggcgcggc gccggtgtag cctgcccggc atgatcgctc
     1441 cgtccgccgc tcgcccgacg ctgcctcgcc acgcgccgct gatcgcggtg ctgcgcggcg
     1501 at
//
LOCUS       DASVOR010001204         2180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_508827,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001204 DASVOR010000000
VERSION     DASVOR010001204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2180
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508827"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF516_22815"
     CDS             <1..244
                     /locus_tag="VF516_22815"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX8110589.1"
                     /translation="LIWRDAEELANRLREPARLERAIGVIYRPDTERMSHYFGAQLPD
                     QFDAVLHFDDTRAVEPLERTSQWEEGEFPETFPFAV"
     gene            732..2162
                     /locus_tag="VF516_22820"
     CDS             732..2162
                     /locus_tag="VF516_22820"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8110590.1"
                     /translation="MENPVRESADKRSTTTQVRDDARAPAQEPGKRSLTEMLPTGDRA
                     PIQRAATAAEPDTADIHASAARGIATPASPLPHADHIQRLFGRHDISGIKAHTGAAAT
                     ASAREAGAQAYATGDHVVLGDKADLHTEAHEAAHVVQQRGGVHLKGGVGEVGDAYEQH
                     ADQVADRVVRGESAEGLLDQFAGGHAGSASAGGVQRKPFRPIPEPGMPVWLRTGGEMV
                     EHKVESYFASAKKVKLVGVPTMVDEATLKYSRPLGQNHMADESEDADWQAQPEPRPRS
                     RYSFAPLVQNKMTVSVTMQKSQAAGHSHTVFEWWDPFSRTSHHHVFEFNPKLNASNQE
                     YALAAKYNGVPGEMRENTNRNYLPNHKDGKPIETWFRSWEVPYKAGIQGKRAAEGFQV
                     LAGSNVVTYQLTGGSMTNAYNCHTMAKRLAEDAGIEVDTGILDGLFQFIPAMFSGYPL
                     DGSEEQMAAAATAKAGPPAAKQADEG"
BASE COUNT          401 a          723 c          756 g          300 t
ORIGIN      
        1 cctcatctgg cgcgacgccg aggagctcgc gaatcgcctg cgcgagccgg cgcgcctcga
       61 gcgggcgatc ggcgtgatct accggcccga caccgagcgc atgagccact acttcggcgc
      121 gcaactcccg gaccagttcg atgcggtgct gcacttcgat gacacgcgcg cggtcgagcc
      181 gctcgagcgc acgtcgcaat gggaagaggg cgagttcccc gagacgttcc cgttcgcggt
      241 ctagctatcc cgagcgggac gtaccacttc gagcgcgacc acgagcaacg acgctgcccg
      301 catgtcgcag tgagcgcgcg ccgagcgagg cggtgccgca tccgctcacc gccggcagca
      361 ccgggcggcg tagaggtcgg cgccgcgctc ccccggcgag gtcatcggct gtcagcgccg
      421 ggaggtccgg ccaacgcgtc tacccttgtc cccgtctttc gcgtagttcc gcagaggtcg
      481 aaccgcctac cgtaaactcc cacgccgttg ctcctgggag ccctacctga aattggtcgc
      541 gcgatcgaat tctttcgtcg agctgtactc cgcaagggcc ctcaccattc cggttcgtgt
      601 cgaagctgtt cggagcgtgc tcctgcgctc atggagcgag atgtggctct tcaccgagtg
      661 gatgcgctgg tgctcgcggc gcagatcctg gatagttcgt cgagcggccg cgccgtcgcg
      721 acggaccgtc gatggagaac ccagtgcgtg aatctgcaga caagcgatcc acaacaacgc
      781 aggttcgaga tgacgcgcgg gcacccgcgc aggaaccggg caagcgctcg ctgacggaga
      841 tgctgccgac gggcgatcgc gcgccgatcc agcgcgcggc gaccgctgca gagccggata
      901 cggcggacat ccacgcgagc gccgcgcgag ggatcgcgac gcccgcctcg ccgctgcccc
      961 acgccgacca catccagcgg ctgttcggcc ggcacgacat ctccgggatc aaggcgcaca
     1021 ccggagcagc ggcgacggcg tcggcgcgcg aggcgggtgc gcaggcctac gccaccggtg
     1081 atcacgtcgt gctcggcgac aaggcggacc tgcacacgga ggcgcacgag gccgcgcacg
     1141 tggtgcagca gcgcggcggc gtgcacctca agggcggcgt cggcgaggtc ggagacgcat
     1201 acgaacagca tgcggaccag gtggccgacc gcgtggtgcg aggcgagagc gcggaaggct
     1261 tgctcgacca gtttgccggc ggtcatgccg gcagcgccag cgccggaggg gtgcagcgca
     1321 agccgttccg tccgatcccg gagccgggga tgccggtctg gctgaggacc ggcggcgaga
     1381 tggtcgaaca caaggtggag tcgtacttcg cctccgcgaa gaaggtgaag ctcgtgggcg
     1441 tgcccaccat ggtcgacgag gccacgctga agtacagccg gccgcttgga cagaaccaca
     1501 tggcggacga gtcggaggac gcggactggc aggcccagcc ggagcccagg ccccgcagcc
     1561 ggtactcgtt cgcgccgctg gtacagaaca agatgacggt ctcggtcacc atgcagaagt
     1621 cccaggcggc cggtcactca cacacggtgt tcgagtggtg ggatccgttc tcgagaacct
     1681 cgcatcacca cgtcttcgag ttcaacccca agctgaacgc cagcaaccag gaatatgcgc
     1741 tcgcggccaa gtacaacggt gtgccgggag agatgcggga gaacaccaac cggaattacc
     1801 tgcccaacca caaggacggc aagcccatcg aaacctggtt tcgtagctgg gaggtgccct
     1861 acaaggcggg tatccagggc aagcgggcag ccgagggctt ccaggtactg gccggcagca
     1921 atgtcgtcac ctaccagctc acgggcggaa gcatgacgaa tgcctacaac tgccacacga
     1981 tggcgaagcg tctcgccgag gacgccggga tcgaggtgga caccggcatc ctcgatgggc
     2041 tgttccagtt catcccggcg atgttcagtg gctaccccct cgatggcagc gaggagcaga
     2101 tggcggccgc ggcgaccgcg aaggcagggc cgccggcggc gaagcaggcg gacgaaggtt
     2161 gagccactca gcggccgcag
//
LOCUS       DASVOR010001205        33541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_187697,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001205 DASVOR010000000
VERSION     DASVOR010001205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 33541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33541
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187697"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..669)
                     /locus_tag="VF516_22825"
     CDS             complement(112..669)
                     /locus_tag="VF516_22825"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8110591.1"
                     /translation="MRPIDLLMREHRLIEQVLDALTEYASRVERGAAPPADDLERFAS
                     WLRGYADAGHHAKEEDVLFVRMVASGIPRDHGPIGVMLEQHEEGRRLVEILLEAAGAG
                     KAWSDAEARRVVRAANDDADLLREHIRAEDDVLYPLAQARVPDAAWDTIAQAFDELEA
                     GRATERATLEALAQALIARYAPGPH"
     gene            768..1754
                     /locus_tag="VF516_22830"
     CDS             768..1754
                     /locus_tag="VF516_22830"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8110592.1"
                     /translation="MNRGNATDASDVSIRPLLESDFEPVVALDRRITGGTRRGYFEQR
                     LTAARRQPTRHVQLAAATPSSIAGFLFARAAGGEFGRVEEALALEAIGVEPTLQHAGL
                     GQRMMAELGALGRARGIGSVVTQAVWRNHEMLRFLDGAGFRLASQQILERAVHRMPLP
                     GTDEEIETLPPLVRHLREGDLDMIARIDKAMTGRDRAAYLKRKVDEALHESPIVVSPV
                     AEDDGFVVAFAMARVDYGDFGHVEPTASLDTIGVNPAFGRRGFARAILTQMIDNMSAL
                     HVERLETEVARDNFALFRFLHRFGFRPSERLPFSRALQAAWSPASARPAREP"
     gene            2341..3564
                     /locus_tag="VF516_22835"
     CDS             2341..3564
                     /locus_tag="VF516_22835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110593.1"
                     /translation="MKKILEPDFDAEGQFLQESLRSPDGELVASGSNATGSGGFTSDA
                     KAAARTLAAGYSEDSVSEDTVDGESLGVILFRIELTLNPTADEQKFHIATSRTGTTFC
                     SGARLLGSFLNRSGDDDQETETQIPIPRPAPPHSCSAARTSLQVRLPDAFAQAVPIES
                     LGFTNFHIGTGGRDRNYRTRRDAAGRRDRWIPVPADLARDRAEAELPGPRRDRRSARA
                     DVLRPATRHHRRLRSRRQPHGCGRGRERRDEHLGSLDILSGRYWKVHREVDEHHLQLG
                     LDRCGDVAPDSPAGIRYQRVLCHAADLHHQMLSSWVGEQAEPIATLWHESVSRITLQP
                     SAGSRRSEHELVGSSHSRHRGIEVTASTRSAAISYSRYHSTRYRSMVNRSSRTVRSRA
                     RSPSESLGSPENQWV"
     gene            complement(4135..4653)
                     /locus_tag="VF516_22840"
     CDS             complement(4135..4653)
                     /locus_tag="VF516_22840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110594.1"
                     /translation="MTTALEHRSVLDDETRGAYIRLWILKKLDLPPDNGGMTFPVVLP
                     AELSPLDSYLQDLAVEGLVAINSRKGRYELTKQGIAYLGAVIDEASDLLDDLDELETE
                     EAIAELRARGLDVYRARFLWGWFQGEFDDLVLFQERRGIMPVERMWAFYLTGDEFWRE
                     LANELTRAEASE"
     gene            complement(4650..5153)
                     /locus_tag="VF516_22845"
     CDS             complement(4650..5153)
                     /locus_tag="VF516_22845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110595.1"
                     /translation="MALTIGQLPFHERPVRELLNLVGERDAPDHDYAGHGWARVPRVW
                     LGHDAGDAGTGRLVADALVLALHSPDDAEPLTDDVELELDLPDGSSVLVLLSRFLAAW
                     LPRLPRASVIVLALCNPYRATPRPPANATAPICYARGDVTSWLDRDRGDRVELLAESW
                     ETVGRMP"
     gene            complement(5158..5913)
                     /locus_tag="VF516_22850"
     CDS             complement(5158..5913)
                     /locus_tag="VF516_22850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110596.1"
                     /translation="MIAKTRLLATKLLAAALAIAFGVGASGCDTSGGEQRTERWATTE
                     NTKIQIDWNAVNEAYKQANGPADLERRINEIYQGDEVISISVADLDARTQIVTGFFDK
                     NADGTVENAEKIFTIRRDITGEGQAQFQTTGHGPFSGYASPFFSIVSGMVVGSMLSNL
                     FMPNYVPAYTQPYTTPSSRIDQLAQSRSSYRAANPEQFQRSRSGRTYDQPSASSRSGD
                     HVGGGRAGGGRIGGGRFGLPRAGRPARPARLEA"
     gene            complement(5967..6671)
                     /locus_tag="VF516_22855"
     CDS             complement(5967..6671)
                     /locus_tag="VF516_22855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110597.1"
                     /translation="MTFATPMSFASCTSFGNPPHSRARSISFGKPPASCAILTSCSKP
                     PRVARRLEERRDPAGVLRKLEELVEAAGLRRHLGERLEAAGFPREIEELGHAAGLLRH
                     LEQLVDPADGLRQLHQLLDAARGLGELDELLDAAHGFRQLDELLDAARRLDQLHQLGD
                     ATRRLGELDDMCDLVCDQPLPGDPDRIVRTARKNDSRRGVRDRPGSSCAPCILQTPWS
                     PGSRRQRASDASSNGQ"
     gene            complement(6781..11502)
                     /locus_tag="VF516_22860"
     CDS             complement(6781..11502)
                     /locus_tag="VF516_22860"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8110598.1"
                     /translation="MRDDKQRTSGRLPSVTSPTGVTALTVDDRPGAAITRDGDGASTA
                     VEELPPLTSRVLGDFVLGEVVGAGGFGTVYRAEQRSLERPAVVKVVHRSLASQPHAGE
                     RFTREARLASRFNHPYAAHVYSFGVEPDGVMWIAMELIDGAPLSRLIRESGPLPLDRF
                     VPLFERLCEVVQSAHDQGIVHRDIKPSNVMVIVRAGRLMPKLLDFGMAKLVGVDTFGA
                     APLGRSPALELGSAPRPSSDVSRSLTRDDQVLGSPMYMAPEQWHDPTTAGPLADQYAL
                     ALVAYEALTGEHAFTGTTLEALAHQHRNAALPALPGHLPAALHAALSRACDKLPERRF
                     ANPTELAMAIRFATLGATSDLEESSASLPEDVSPYPGLAAYTAAEHALFIGRERDLEE
                     LIDRLDTQPIITVVGPSGSGKTSFLAAGLAPALPVGPPAWRAELVRPGGDPPGALWAI
                     VARHDGSGHRKAARDGAATSPAALAAGLMGVAEARGVVLVVIVDQAEELFTMCTEQAG
                     RLAFAEALVMASASRRVRVVLAMRDDFLCRIEQLPAWRGLLGRAVHVLGVPRRDDLER
                     MLTVPARRCGFGFDDPALPREIVDEVADRPGALPLVAFTAAQLWEHRDRRGRRLTRAA
                     YQRIGGVIGALVQHADSVVDRMSIPDRRQVRLLFRRLITAEGARALISRTELESALGG
                     ASAGAVIDRLLAARLIVSRDDDTGDRIEIIHETLATTWPRLASWRREDADGAQLQERL
                     AVAARHWDERARASDLLWRGDALSDLRRWQARGDTSLTPVEQAFVRACITSAARVRRT
                     RIAIVVAAFALLVAGILGLVSANRKIADQRAAAVDRLRASFEERGRLAIADGDAAHGL
                     LYLAEASRLGARGPGFDLLTTQAVASLDAGLEVVAHGSSGIAAMETSPAGFVTLGWDL
                     TLSRWDRAGKETRVADGMYYATVIGDLAVSVSTEGDVVATDAGGSVRWTARHAVADPE
                     TRSGGVAVSTAAHLVVAYGRTATLWDPDTGQSRGELPHDRGVTAVALAGDGTRIATGD
                     SAGVVRIWNTSTVQRIATCEPHTGVIRVLRFSPDGRSVVSGGNDGVVTICDTTSGATL
                     HRLVGHSRQVVAVDVTADGRTIVSAGRDGKPRIWDAQTGLLIRVLEGHHGAVTGAQFS
                     PDGSAILTFGLDGTARIWNSEGMALGSLQGHGGPIYAGRWDADGRHVVTTSVDGAIRR
                     WDPSRAVKTAFRQAHGEAVTDLAISDDDRWVLTASSDGHARLWERRSLQPITDLPHGA
                     KVISARFGPGARALTTDEAGGVRIWQLPEGQLIARLGPGITAAACANDGRIVTATGGS
                     VALWSPTGARLDTAAVGYEVRRLSFDPTGRWLFVQGTASSVAVIDIAAFTPVVRLAVH
                     DHHVLGLATSASRVAITDGPAVRLWQPGTWLPLGTLAGHKSPVADVWFASDERLVSAG
                     ADGTLIWGFSPLRSEKLGDTNRVLALATSPDGTLFATTAADGATRIWGATSYRLLLQL
                     PSHSLPAFALQFAHDGASAISGGNDGRLVTWDLTRRTRSSSELAEIVRCRVPLRLEGD
                     VALPRELDFDDPACRSLVLDR"
     gene            complement(12205..12369)
                     /locus_tag="VF516_22865"
     CDS             complement(12205..12369)
                     /locus_tag="VF516_22865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110599.1"
                     /translation="MREHDHELATHAFRYSGGRMVDLGVPPARLGIATSSSANDINDA
                     GVIVGNSGTL"
     gene            12551..13312
                     /locus_tag="VF516_22870"
     CDS             12551..13312
                     /locus_tag="VF516_22870"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HEX8110600.1"
                     /translation="MTLQSALVCALASCSSAAAAPEASAGTSIDQAAPDGTATSATAA
                     ASAYFSIRADLRKCAYPMCGGWFLSRVNSATTQCHDGTIATSCYTPVLDWTNSGLDDT
                     QQAALSEAANHAAVSPGVYAIVVGRFARTNTTTPDPTLGRFVISQAWVAEGDGVSSGT
                     FVKVQDNGLRCFAAPCPNLTETTLNTPQTTDIAEVDWTPSGLTEAQIEDCTQDMYGPD
                     GLVIAGDRYTTTVNGTAAQGRTVTDAYQRLVPQPQ"
     gene            13309..13560
                     /locus_tag="VF516_22875"
     CDS             13309..13560
                     /locus_tag="VF516_22875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110601.1"
                     /translation="MSVSRDLHSHPDLSRDAWHGTVIALFQPAEETSEFHVVEVEPTF
                     GEHQCATVFAPLHASPSEIEAARPPRAVFDPYPMTQRKA"
     gene            complement(13833..14048)
                     /locus_tag="VF516_22880"
     CDS             complement(13833..14048)
                     /locus_tag="VF516_22880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110602.1"
                     /translation="MVGHVTVGRSRLGNLRTDLARCQAGGGPRLAFGAARDALGGALL
                     LILLALPLVRRGFLIGWHYLALPVQDP"
     gene            14079..14393
                     /locus_tag="VF516_22885"
     CDS             14079..14393
                     /locus_tag="VF516_22885"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX8110603.1"
                     /translation="MAGRSRPEQSPTARRIYVGNLSYNMDANSIASLFAQHGEVADVH
                     IVLDQVSRRPRGFAFVTMGSPAEAQAAITELDGQNVDGRTLRVNQAEARESGPSMGRG
                     RY"
     gene            complement(14604..14990)
                     /locus_tag="VF516_22890"
     CDS             complement(14604..14990)
                     /locus_tag="VF516_22890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110604.1"
                     /translation="MKIRTIKDPRLDAKPAPAIEPPITGKFQPALVHPRASSEPDLES
                     RIRLRRAELFVRLRELREDRRLEAAEARDKVKAKLSELTHLLKWGISDGWGSVGDAVK
                     HRLERWLRESMHQLPIRDRPDKTGQA"
     gene            complement(15008..15727)
                     /locus_tag="VF516_22895"
     CDS             complement(15008..15727)
                     /locus_tag="VF516_22895"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8110605.1"
                     /translation="MISLRRAEARHHDPHRRRKVWRTFDPRDQADGLAEGFGTIEAID
                     ENRLPPRGDVPGYAAEQAEIVTYVREGTIVYEDAIGHSGAVHAGEFQRIIAALGTRHS
                     ETNASRADWAHVFQIRLRRTAVALEPGREQRRFSVAERRGGLWLVASPYARSGSLQLH
                     HDTWIYSALLDPGQHVVHKLTPGRAVWLHIVQGEVAFGDLVLTTGDGAGVTAERSVSL
                     TAREGTEILLLDVVEHQARGP"
     gene            16048..17721
                     /locus_tag="VF516_22900"
     CDS             16048..17721
                     /locus_tag="VF516_22900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX8110606.1"
                     /translation="MARADELDHKELLELDPDGGVIRFAGQRALLLDAVAIGLLRKYL
                     VENFGITAARTVLTQFGFAHGWRMAEAMRAEFPWESDDEWRSAGTRIHALEGLFHVAP
                     GGSGPLSRTGSMIVASYEAEQHLLHFGRSDVPVCWTICGLTSGYLSRIAEKHMYVLEH
                     GCASKGDAACHLFARSREEWGGEHAEELHFFEPSRLEECLDVSLQRVTRTLQAAERKL
                     HRHRRALVQVARDVEEPLGIVTRSVTMRRVVELARRVAKVDSTVLITGESGSGKERIA
                     RLLHDVSTRATGPFIAVNCGAITETLLESELFGHARGAFTGATQDRPGLFEAANSGTL
                     LLDEVGELSPAMQVKLLRALQEREIRRVGENKSRHVDVRVVAATNRDLAHGVAGGGFR
                     QDLYYRLKVVELHVPPLRERRDDILPLARVLLADAALRMKRKITGLVPNAADQLLRYG
                     WPGNVRELENVMERAVALACESRVELEDLPEEIRHTLPRGTAGGVVRPLDEVEKEYII
                     AVLALNEGNQTRTAKQLHIGSATLYRKLKSYGLLGKARTRNSRPRGDSM"
     gene            complement(17732..18016)
                     /locus_tag="VF516_22905"
     CDS             complement(17732..18016)
                     /locus_tag="VF516_22905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110607.1"
                     /translation="MNKGIAMSINYGDKSVATLRCLVTAIECQFALVPPPEKVRAAWA
                     ELVDVLALGPSPELRTCPTCGEIGMRAATRCMRCWSSLPPLPPQTTPGRR"
     gene            18441..18824
                     /locus_tag="VF516_22910"
     CDS             18441..18824
                     /locus_tag="VF516_22910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110608.1"
                     /translation="MTTSTTNDDPPAPDAKPDAAAIGPPIARETQPELAVRAAATEAD
                     LESRIQQRRGELIATLGQLRTDTHLAATEVRGRLKAQLSELAQIIKVGVVDGWTNLGD
                     TVKYKLEQWLAESERSMKDLPAKNG"
     gene            18991..19314
                     /locus_tag="VF516_22915"
     CDS             18991..19314
                     /locus_tag="VF516_22915"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX8110609.1"
                     /translation="MRTRDIVPPFHATDDLISRRMAQCGEVRGVDLILDRVTGRPRGF
                     AFVEMATAEGMQKAIAELDGKDFEGRSLTVKVAEERGGGGPRGNGGPRRGGDREGDRG
                     WGRGR"
     gene            complement(19642..21096)
                     /locus_tag="VF516_22920"
     CDS             complement(19642..21096)
                     /locus_tag="VF516_22920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8110610.1"
                     /translation="MKDSHAVVIVGGGPTGLMLAGELALARVGVAIVERRESQELAGT
                     RAGGLHARTIEVLDQRGIADRFLSRGQTMQIAHFAGPLDISDLPTRHNYGLALWQNHI
                     ERILADWVGELAVPIYRGREVTAFAQDDTGVDVALSDGRSLRAKFLVGCDGGRSLIRK
                     NAGIDFVGWEPSTSYLIAEVEMTDEPAWGIRRSERGTHALGKVEDARLVRVVLEEPHV
                     GHGEPTLAEVREALVAAYGTDFGAHGATWLSRFSDAARQASSYRDRRVLLAGDAAHVH
                     SPVGGQGLNTGVQDAVNLGWKLAQVVHGTSPESLLDTYQAERHPVAARVLRTTMALTA
                     LHRGDERSKALHESMAELLRMNEPRKRYGAMMSGLDIHYDLGAGHPLLGRRMPDLDLV
                     TDAGPLRVFTLLHDARPVLLHLGGAFDITPWADRVRQIDARYDGAWELPVLGAVAAPT
                     AVLIRPDGHVAWVGEGTDHGLRDALATWFGSPVR"
     gene            21993..24788
                     /locus_tag="VF516_22925"
     CDS             21993..24788
                     /locus_tag="VF516_22925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8110611.1"
                     /translation="MTGSRIGDPLGKQAPVLMMSREDIERTGLTSVGDILQQLPVSGG
                     ALNGKYNSSGNFGYPPDGGGIGAGATEADLRYLGSKRVLVLVDGVRWVNGSSASGVSA
                     STDLNTIPLGIIERIEVLEDGASPLYGSDAIAGVINIITRKELSGVRASAYTGAFGQG
                     DGVVQKYDLSWGGTTGKLSTVVGGSFVDQRAVSSGDRSISSFPVPNVGRCTSNCSGTG
                     GHGRVIYFDPNGTKHDLALEDGTGGMFHTFTNDDRFNYQPYNYAETPSQRVNIFSALT
                     YKLSPDINVRGKASFNNRESVNQAAPEPLTIGPGAHTNSRLDRISVDATNPYNPFGFT
                     FDPMTDTGYQIDRRPVEAGPRIFQQDVHTFYSSGGLDGHFDLGGQRFTWDTTVAYGLN
                     RAEQRRNNAFNSAKLQQALGPAYLGSDGRYHCGTAANPGDPNCVPFDLFDGQGADGRG
                     TITPAMLAYTTFTEHDVSEQTMVDSVANLSGNLVKLPAGWVAAAVGVEHRRLSGFYEP
                     DSVIAAGDGAGAVSSPTSGHYSVNEAYGELRAPLLAGMTGAQLLDVDVAGRVSDYSFL
                     SPKFTGKVSARWKPITDMVLRGSFGSGFRAPSIGELYGSKARFNPILNDPCSDFNKSG
                     VSAAVRQRCIDLGVPSNGSYVQANQQIGVTTGGNRALSPETSTSINLSLAYSPAWLQD
                     RPWVDSLDLELAYYDIRLDSAVAALDAQLQLNQCVLNADDKACMGVARSPEGAISAFD
                     NVLQNIGGINVRGLDLTLAYRMPRQSFGRLRATLQSSYLLAYETKTPSAGGFQTTDLV
                     GIISGTPTHAFPRFKSNLALSWLHKTLDVTLITRYIHSVTEHCPGLAAFPGTCSNPNP
                     DDTKSTNKLGITVYNDVQVVWSPEFDRRLTVTAGVNNLLDRNPPNCFSCSLNGFEGTT
                     YDVPGVFGYLTATYHMQ"
     gene            complement(25052..28324)
                     /locus_tag="VF516_22930"
     CDS             complement(25052..28324)
                     /locus_tag="VF516_22930"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110612.1"
                     /translation="MEPGKDEDVTVALGPAPPRDDQPPPLPFQVRDQQRYHFFGEHGR
                     GGLGRVSRVRDRELGRDVAIKELLSRGSADEPRFLREVLITSRLEHPGIVPVHDAGRW
                     AQGTPFYAMKLVAGRPLRELIAERARVEDRIGLLHHVIAVADAMAYAHGRNIIHRDLK
                     PANVVVGEFGETVVIDWGLAKDLSSSEPAGIESAPSGPSPSPSPGDDLTVAGAVLGTP
                     AYMAPEQKRGEPVDKRADVFAIGMMLWELCAPQRIPPGEAHLRHRMLRRAGIDEDLAT
                     ITEKALDPDPERRYPDAGALAADLKAFKAGARIAARSYSLLAMLTHWTRRHRALALAT
                     AAAAAVAVAGTALYVRNIATERDRADASQASAERARASAERARASAQATLDEFTLKHA
                     ELLLATDPSAAVDALATYHGTDLDRAAQIRAEAAGRGVAVLRALPHSENVRWIQGVPN
                     GEILSLSHDGTIARTSRDGRSTVVARNVSRSGIWAYAQSRHLLAYACNPSALCLLDAL
                     RAALIPTAAPLRDANIDGLDFSPSGNLLGMMSHDAVLRVFDVTDPARPALRLTRPIAG
                     GYDVVFISDDIAVAGAPNGIEFIRMNGTSERYSLPGFWRWAAGRTEHQIALTTKQGEA
                     VILDSFPARVVARTTLCHGPTAQLQFIPGRQSIAYACHDGALGTWDLQHGTVRPRAQL
                     EGHADFIADGATGDYVIAAGGNGIITVLDLETDLIASYRGHGVRLTWLASPTPEQPFI
                     ISGDVRGAIRVWPLPARLARVAATSRSPFYRAIFDEHSAMVTATTLLPALTTFSPATG
                     VRAVEPHLSDNIFLERSASGRTFATYGVNDLVEVWSAASMTRARVIPTGHGSVTQLRF
                     VDDTDDFITSGHDGRVVRWTPSGQPTLLVESSAPIDRFATTRTAGSILFSTVDGALWR
                     AGGDGHVFSLRIGGSRVSRLLTLPDQQTAIAGYANGDVVEIDTRSWQQVIVLHGSGPV
                     GDIAVTGDGRTVAVATNDGAIHVGTRRAASGPEPPTWLTLAGRANHVALALDLVMASC
                     TDGTIWLYSLARRRWLYLPTGTVDISQIAVIADGKAAVALDREGRLLWMDLEAARKLL
                     DT"
     gene            complement(28388..28642)
                     /locus_tag="VF516_22935"
     CDS             complement(28388..28642)
                     /locus_tag="VF516_22935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110613.1"
                     /translation="MSVVLDIRPVAGPCRVAAPDLANLAWNKLGFAQLGDDGPDRQRW
                     QVPREANLSGGALHVGTGCADPGAMEHLAAPVWVVTRCLV"
     gene            29341..31665
                     /locus_tag="VF516_22940"
     CDS             29341..31665
                     /locus_tag="VF516_22940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110614.1"
                     /translation="MNLVKHTATPAVRPRPVAAVRELPTLVVSALACSDHDVEFDCRY
                     QVAWSINPHMAIGAVDFPTAALQHSMFIAALAAAGARIVPLPFVHGAYDSVFAKDSAL
                     LLERRGLRRALLARLRYPERQREQAARADFYERYGYEVVWEASGPSWEGGDTVMLPSG
                     HGMFLGYGPRSSCEAAQWLERHAGVAVTPLALRDPHLYHLDMALAVLPDGTALVCETA
                     LTADSMHVLERASGIREVVTIPRADALAFGLNLVPIGDTIICGAWVPRVAAIIEARGY
                     RCQVVPLDQFHLAGGSAACLVATVHRDPIAPRKPSTPVHSRGTMDIYGPLFRSVLFPV
                     WERYVRQRPVVERWLELKRTQWMSLDELTMHQTRALQHLIHHAYAHVPFYRARFDALG
                     LMPDDIRSPADLLQLPVIRRSDLQSRSIERASRVPPLPTIRKQTSGTTGEPLLFAFEP
                     NSEHWRRAVKLRGYEWAGYRPGDRALHFWGAPHPSEPPWSTRIKIALDRKMNRDIYIP
                     CAVMSEDRLRDVVRVIERVKPQVLVCYAQAGAELARYIHRNGLRSWQTIPVICGAERL
                     LPRDRADLEDAFGPSVFDTYGCREVMMIASECEAHHGLHIAMENLVVEIVVTEDGRQR
                     LAREGESGEVVFTDLHNYGMPFIRYANGDIATAGQTRRCSCGRTLPRIQSIQGRMSET
                     LRDGSGAPVSGIALSFLFQDISSAVRQFQAMQHKDRSVTISVVQSEPLSSQMIEDVRR
                     NGQRLLAGVDVRVVVVPELPRNPAGKHNLVVVER"
     gene            complement(31666..32445)
                     /locus_tag="VF516_22945"
     CDS             complement(31666..32445)
                     /locus_tag="VF516_22945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110615.1"
                     /translation="MLLALGIVSLLPVAGWADEEHTPDDASAVVQKYLDARVKKEGVF
                     RFKDAQADAQLELVPEQIRVARGIHGYGFFVCAQFHTKADAKKPYDIDFWLKEDSLDV
                     VDVRIHKAPKRDGDKWTLVTRNPLLWWWIPASEHPGEFEEKRGWQIEAAINGYIAKQA
                     KDGVFTVKDDKTGEQRTLNFVEIHRPMRKMEGKGYFACTDFREHKSANKYYDIDFWLA
                     DKNGTLEVTEVRIHKEPKQEDGAWIQVPRYSFEKGKTKELP"
     gene            32731..>33541
                     /locus_tag="VF516_22950"
     CDS             32731..>33541
                     /locus_tag="VF516_22950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8110616.1"
                     /translation="MAASPRVSVVIPAFQSRWLDQALDSVRAQTFPSYEIVVVDDGSP
                     VPLQPAKRDDVVVVRQTNTGPGGARNRGVAVARGELIAFLDSDDRWRPQMLERQVAWM
                     DANPTAVLVFTGVHLDGRNPRDESYAHLLDPRSGRLPYGVLFEENMIPTSCAMIRRQA
                     YLRTPGMSLHRRLGEDYALWLRIALLGEVGFIDEVLLDRRVHTGSLMAGASRDATIEA
                     DLEIYEELFAEHPELRGAFAPRALARVEFQRGYECLATARWAGARQAFARSL"
BASE COUNT         5319 a        11527 c        11462 g         5233 t
ORIGIN      
        1 ccccatggcg gcgctcgctg gcgccatcgg tgcggcgatc ctcggcgcgt atcgcttcga
       61 ccagctggag cagcgaggtc gcacggtggg cgcgtcggcg tcggggccag cctagtgcgg
      121 accgggagcg tagcgggcga tcagcgcctg tgcgagagcc tcgagggtgg cacgctccgt
      181 ggcgcggccg gcttcgagct cgtcgaacgc ctgcgcgatc gtgtcccacg cggcgtccgg
      241 cacgcgcgcc tgcgccagcg ggtagaggac atcgtcctcg gcgcggatgt gctcgcgcag
      301 cagatcggca tcgtcgttgg ccgcgcgcac gacgcgtcgc gcctcggcgt cgctccacgc
      361 cttcccggcg ccggcggcct cgagcaggat ctccacgagc cgccgcccct cctcgtgttg
      421 ctccagcatg acgccgatcg gaccatggtc tcgcgggatc ccggacgcga ccatgcggac
      481 gaacagcacg tcctcctcct tggcgtgatg cccggcatca gcgtacccgc gcagccagct
      541 ggcgaaacgc tccagatcgt ccgccggtgg ggcggcaccg cgctcgacgc ggcttgcata
      601 ctcggtgagc gcgtcgagga cctgctcgat gagtcggtgt tcacgcatga ggaggtcgat
      661 ggggcgcatg accggtcgag cgtcgagagc ttatcgatcc tctgcgcgag ccgccaggac
      721 aatcgcggcg gctccacccg gaaggttcca ccgaagcgag gtgcccgatg aaccgtggca
      781 acgcaaccga cgcgagtgac gtgtccatcc ggcccttgct ggagagtgac ttcgagccgg
      841 tcgtcgcgct cgaccggcgc atcacgggtg gcacgcgccg cggctacttc gagcagcgac
      901 tcacggcggc tcggcggcaa cccacccggc acgtgcagct cgccgcagcc acgccgtcca
      961 gcatcgccgg gttcctgttt gcgcgcgccg cgggcggcga gttcgggcgg gtcgaggagg
     1021 ccctcgcgct cgaggcgatc ggcgtcgagc caaccctgca acacgcgggg ctcggccagc
     1081 gcatgatggc tgaactcgga gcgctcgggc gcgcccgcgg catcggaagc gtggtcacgc
     1141 aagccgtgtg gcgcaaccac gagatgctgc ggttcctcga tggcgcaggg tttcgcctgg
     1201 cgtcgcaaca gatcctggag cgagccgtgc accggatgcc cctcccgggc acggacgagg
     1261 agatcgaaac gcttccgccg ctcgtgcgac accttcgcga aggtgatctc gacatgatcg
     1321 cgcgtatcga caaggccatg accggtcgcg atcgtgctgc ctacctgaag cgcaaagtcg
     1381 acgaagcgct ccacgagtcc cccattgtcg tctcgcccgt cgccgaggac gacggcttcg
     1441 tcgtcgcctt cgcgatggct cgtgtcgatt acggcgattt cggtcacgtc gaaccgaccg
     1501 cctcactcga cacgatcggc gtgaaccccg cgttcgggcg cagaggcttt gcgcgcgcca
     1561 ttctcacgca gatgatcgac aacatgtcgg cactgcacgt cgagcgcctc gagaccgagg
     1621 tggcacgaga caacttcgcg ctgttccgtt tcctccaccg gttcggcttc cgtccgtcgg
     1681 agcgcctacc attctcgcga gctctacagg ccgcgtggtc gccggcttcg gcacgccccg
     1741 ctcgcgaacc ttgatcagcg gaccgaccgc aaccgtgaat ggcaccggca gcattcgtcg
     1801 atccgcttgt gccctaccca tgcgggcgat cgcgcaacgg cgcaggcctg aactcgacgc
     1861 gcgggcggtc ctcgcgatga tcgcggacga ccgctgcctg ccggacgggg tgcggcgagc
     1921 gacccagcct tgtggcttgt gggcggtagt gcacatactg gtgcggtcgc tcgatcctta
     1981 gcaccggtac cggcggcgtc gagtagacct gtccactggt gtggacggcg gtgcgccgct
     2041 attcgctatc gcttccggtg cggcgctcac tccgattgcg cgccgtgccg atctcgtgct
     2101 cgagcatcgg caggacttcg caaagcgcac cgtacccgaa atccgcatgg gaaacagact
     2161 catcttattg tacgcgcttg ggacatcacc actgcgaagg ttggcacgat gcgacagctc
     2221 cgctcgcgga ccttcgtgaa atcaagagcg gcgtcgaagc acagatcttt ctcgcccgtc
     2281 atgaggctcc cacatggcgg ccgcaagtgg cgtacctggt acgtgaatgc agccggaagc
     2341 gtgaagaaga tcctcgaacc cgatttcgac gccgagggcc agtttctaca ggagtcgctt
     2401 cgcagccccg atggcgagct cgtggccagc ggcagcaacg ccacgggctc tggtggcttc
     2461 accagcgacg cgaaggcggc cgcacggacg ctcgccgccg gctacagcga ggacagcgtc
     2521 agcgaagata cggtagacgg cgagtcgctc ggcgtcatcc tgtttcgcat cgagctgacc
     2581 ttgaatccga ccgcggatga gcagaagttc cacatcgcca cctcgcgaac cggaacaact
     2641 ttctgctctg gggcgaggct ccttggcagc ttcctgaacc gctccggcga cgacgaccag
     2701 gaaactgaaa cccaaatccc cattccccga cctgccccgc cgcacagctg tagcgcagca
     2761 cggacatcgc tgcaggtccg cctgccggac gcgttcgccc aggcggtgcc gatcgagagc
     2821 ctcgggttca cgaacttcca catcggcacc ggggggcgtg accggaacta tcgcactcgt
     2881 cgggacgccg ccggacggcg cgataggtgg attcccgttc cggccgacct cgctcgcgat
     2941 cgagctgaag cagaactgcc tggtccgcgc cgagatcgaa ggtcggctcg cgctgacgtt
     3001 cttcgaccag ccactcgaca tcaccgccgg cttcgatctc ggcggcaacc tcacggctgc
     3061 ggtcgcggcc gagagcgacg cgatgaacac cttggatcgc tggacatcct gagcggacgc
     3121 tactggaagg ttcatcgcga ggtcgacgag caccacctcc agctcgggct cgaccgctgc
     3181 ggcgacgtcg cgcccgactc accggcgggg atacgctacc agcgggtgct gtgccacgcc
     3241 gccgaccttc atcaccagat gctcagctcg tgggtcggcg agcaggcaga gccgatcgcg
     3301 acgctgtggc acgagagcgt ctcgcgtatc acgctgcagc ccagcgccgg atcacggcgt
     3361 tcagaacacg aactcgtagg cagcagccat tcgcgtcacc gcggaatcga ggtcaccgcg
     3421 agcacgcgat ctgcagcgat ttcgtacagt cggtatcatt ccacccgcta ccgatcgatg
     3481 gtgaatagat catcccgcac tgttcggagc cgtgctcgct ccccctcgga gtcgttgggc
     3541 tcgcccgaga accagtgggt gtaaccaagc ggtgttccat cgagccaggt ccagtcatcg
     3601 gccggcatcg ctgcacccgc ggcctggacc agcccgatcc aggtgaatcc ctggatcggg
     3661 acgatcgatt cgaggcaatt ctcctcggtc gcatcgcgca ccgacgcgag cgtccgcccc
     3721 acgcggcaca cgcagccacc gccgtcgcgc ggtcgagcag gtcctcgcag gtcgtgtagc
     3781 acttgccgtt gcagtcgatg aactgcgcgg cgccgctgca cgcgatcggt gcgcacggcg
     3841 ccgcgtccgc ggtggccggc gcgctcgcat cgctcgacgt gctcgacccc gaaccagcgt
     3901 cgatcgcggc gaagtcgacc cgcccacatc cggcgctggc gacgaccgtc atgacgaccg
     3961 tggcaacacg cgaccgcacc tggtcagtat acacccggca accgcagcca agtcccccgc
     4021 ttgcgcccgc ttcgctcgtg ctcgcgctcg ccgcttcgcg gctcaggtcg cttcgcgccc
     4081 ggggtcctct ccgtcccctt cggggtagcg agtcccccgc tcgcaccgtg ttcctcactc
     4141 gctggcttcc gcgcgcgtca gctcgttcgc gagctcgcgc cagaactcat cgccggtcaa
     4201 gtagaatgcc cacatccgct cgacgggcat gatgccgcgg cgctcctgga acagcacgag
     4261 atcgtcgaat tcaccctgga accagcccca caggaaccgg gcgcggtaca cgtcgagtcc
     4321 ccgggcgcgc agctcggcga tcgcttcctc ggtctcgagc tcgtcgagat cgtcgagcag
     4381 gtcggaggct tcgtcgatca cggcgccgag gtaggcgatg ccctgcttcg tcagctcgta
     4441 gcgacccttc ctggagttga ttgcgacgag gccttcgacc gcaaggtcct gcaggtagct
     4501 gtcgagcggc gatagttccg ccgggagcac gaccgggaac gtcatgccgc cgttgtcggg
     4561 cggcaggtcg agcttcttca ggatccacaa cctgatgtac gcacccctgg tctcgtcgtc
     4621 gaggaccgag cggtgttcga gtgcggtggt catggcattc tcccgaccgt ctcccaggac
     4681 tcggcgagca gctcgacacg atcgccgcgg tcgcgatcga gccagctcgt gacgtcgccg
     4741 cgcgcgtagc agatcggtgc cgtcgcgttg gcaggtggac gcggggtcgc gcggtacggg
     4801 ttgcacagcg cgagcacgat caccgaggcg cgcggcagcc tcggcagcca cgccgccagg
     4861 aaccgcgaca gcaggacgag caccgacgag ccatccggca gatcgagctc gagctcgacg
     4921 tcgtcggtca acggctcggc gtcgtccgga gaatgcagcg cgagcacgag cgcgtcggcg
     4981 acgagacggc cggtgccggc gtcgcccgca tcgtggccga gccacacgcg tgggacgcgc
     5041 gcccagccgt ggccggcgta gtcgtggtcg ggtgcatccc gctcgccgac gaggttgagc
     5101 agctcacgca ccggccgctc gtggaacggc agctggccga tcgtgagcgc cacagcgtca
     5161 cgcctcgagg cgcgccgggc gggccgggcg gccagcacgc gggaggccga accgaccacc
     5221 gccgatccga ccgccgccgg cccgaccgcc gccgacatga tcgcccgacc ggctgctcgc
     5281 cgatggctgg tcgtacgttc ggccgctgcg cgagcgctgg aactgctcgg ggttcgcggc
     5341 gcgatagctc gatcgggact gcgcgagctg atcgatgcgc gagctcggcg tcgtgtacgg
     5401 ctgggtgtac gcgggcacat agttcggcat gaacaggttc gacagcatcg agccgaccac
     5461 catgccggag acgatgctga agaacggcga ggcgtagccg gagaacgggc catggccggt
     5521 ggtctggaac tgcgcctggc cttcgccggt gatgtcgcgc cggatcgtga agatcttctc
     5581 ggcgttctcg acggtgccgt cggcgttctt gtcaaagaac ccggtcacga tctgtgtcct
     5641 ggcatcgaga tcggcgaccg agatcgagat cacctcgtcg ccctggtaga tctcgttgat
     5701 ccggcgctcg aggtccgccg ggccgttcgc ctgcttgtac gcctcattga ccgcgttcca
     5761 gtcaatctgg atcttggtgt tctccgtggt cgcccagcgc tccgtgcgct gctcgccgcc
     5821 ggacgtgtcg caaccgctcg caccaacgcc gaatgcgatc gcgagcgccg cggccaggag
     5881 cttggtggcc aggagcctgg tttttgcgat catggccgaa ccctagcctc ggactccccc
     5941 gagtcaacca ggcgcgtcgg gctgcctcac tgcccgttcg aggaggcgtc agacgctcgt
     6001 tgccgtcgcg agccagggct ccatggtgtc tggaggatgc acggagcgca cgaactcccc
     6061 ggacgatctc ggacgcccct ccggctgtcg tttttgcgcg cggtgcgtac gatccggtcg
     6121 ggatcgccgg gcaacggctg atcgcacacg aggtcgcaca tgtcgtcaag ctcgccgagg
     6181 cggcgggtgg catcgccaag ctgatggagc tggtcgaggc ggcgggcggc atccagaagc
     6241 tcgtcgagct ggcgaaagcc gtgggcggca tcgagaagct cgtcgagctc acccaggccg
     6301 cgggcggcat ccagaagctg atggagctgg cgcaggccgt cggcgggatc gacaagctgc
     6361 tcgagatggc gcagcaggcc ggcggcatgg ccaagctcct cgatctcgcg cgggaagccg
     6421 gcggcttcga ggcgctcgcc aagatggcga cggaggccgg cggcttcgac aagctcctcg
     6481 agcttgcgaa ggacgccggc gggatcccgg cgctcctcca ggcggcgcgc gacgcggggg
     6541 ggcttcgagc agctcgtcaa gatggcgcag gaggccggcg gcttcccgaa gctgatggac
     6601 ctcgcgcgcg agtgcggcgg attgccgaag ctcgtgcagc tcgcgaagga catcggcgtc
     6661 gcgaaggtca aggagtacct cgatatcctc aaggccggcg agcgcatcag cgatccggtg
     6721 aagacacgtg acgcggtccg cggaggcatg cgggtatgcc ggcggcaagc agcctcgatc
     6781 tcatcggtcg agcacgagcg atcggcacgc tggatcgtcg aagtcgagct cgcgcggtag
     6841 cgcgacgtcg ccttcgagcc ggagcgggac gcggcaccgc acgatctcgg ccagctccga
     6901 cgacgagcgc gtccggcggg tgaggtccca ggtgacaagg cggccgtcgt tgcctccgct
     6961 gatcgcgctg gcgccgtcgt gggcgaactg cagcgcgaac gcgggcaggc tgtgactcgg
     7021 cagctgcagg agcaggcgat agctcgtcgc accccagatc ctggtggccc cgtcggcagc
     7081 ggtcgtggcg aacagcgttc cgtcaggaga tgtcgccagc gcgagcacgc ggttggtatc
     7141 tccgagcttt tccgagcgca gcgggctgaa tccccatatc agcgtgccgt cggcgccggc
     7201 gctgaccagt cgctcgtccg atgcaaacca cacgtcggcg acaggactct tgtgaccggc
     7261 cagtgtgccg agcggcagcc aggtgccggg ttgccacagg cggaccgcag ggccatcggt
     7321 gatcgcgacc cgcgatgcgg aggtggccag accgaggaca tggtggtcgt ggaccgcgag
     7381 gcggacgacc ggcgtgaacg cagcgatgtc gatcacggca acggagctgg cggtgccctg
     7441 gacgaatagc cagcggcccg tcgggtcgaa gctcaaccgc cggacctcgt agccgacggc
     7501 ggcggtgtcg agccgcgcgc cggtcgggct ccacaacgca acgctgccgc cggttgcggt
     7561 gacgatgcgg ccgtcgttcg cgcaggccgc tgcggtgatc cccggcccga gcctggcgat
     7621 cagctggccc tccggaagct gccagatccg gacgcctccg gcctcgtcgg tggtgagcgc
     7681 gcgggcgccg gggccgaacc gggcggagat caccttcgcg ccgtgcggga gatcggtgat
     7741 cggttgcagc gatcggcgct cccacagcct ggcgtggccg tcggagctcg ccgtgagcac
     7801 ccagcggtcg tcgtcggaga tcgccagatc tgtgaccgct tcgccgtggg cctgccggaa
     7861 cgctgtcttg accgcccgcg atggatccca gcgccggatc gcgccgtcga cgctggtggt
     7921 caccacgtgg cggccatcgg cgtcccatcg cccggcatag atcggcccac cgtgcccctg
     7981 cagcgaaccg agcgccatgc cttcgctgtt ccagatccgg gccgtgccat cgagaccgaa
     8041 ggtgaggatc gcgctgccgt cgggtgagaa ctgtgcgccg gtgacggccc cgtgatggcc
     8101 ttcgagcacg cggatcagca ggccggtctg cgcatcccag atccgaggct tgccgtcgcg
     8161 acccgcggac acgatggtcc tgccgtcggc ggtgacgtcg acggcgacga cctggcggct
     8221 gtggcccacc aggcggtgca gcgtcgcgcc gctggtggtg tcgcagatcg tcacgacacc
     8281 atcgttgccg ccgctcacca cgctgcgtcc gtcggggctg aacctgagca cccggatcac
     8341 cccggtgtgg ggctcgcagg tggcgatccg ctgcacggtc gacgtgttcc atatccgtac
     8401 cacgcctgcg gaatctccgg tggcgatgcg cgtgccgtcg ccggcgagcg cgaccgcggt
     8461 gacgcctctg tcgtgaggaa gctcgccgcg agactgaccg gtgtcggggt cccacagcgt
     8521 cgcggtcctg ccgtacgcga ccacgaggtg ggccgccgtg gacaccgcga cgccgcccga
     8581 tcgggtctcc ggatcggcca cggcgtgcct ggcggtccag cgcacggaac ctccggcatc
     8641 ggtcgcaacg acgtcgcctt cggtcgacac cgagaccgcg agatcgccga tcaccgtggc
     8701 gtagtacatc ccgtcggcga cccgggtctc cttgccggct cgatcccatc gcgacagggt
     8761 gaggtcccag ccgagcgtga cgaaccccgc cgggctggtc tccatggccg cgattcccga
     8821 gctgccgtga gcgacgacct ccagcccggc atcgagcgac gccacggcct gtgtggtcaa
     8881 cagatcgaag ccgggccccc gcgcgccgag ccggctggct tcggcgaggt agagcagtcc
     8941 gtgggcggcg tcgccgtcgg cgatcgcgag ccggccgcgc tcctcgaagc tggcgcgcag
     9001 gcgatcgacc gcggcggctc gctggtcggc gatcttgcgg ttcgccgaca ccagcccgag
     9061 gatgccggcg acgagcagcg cgaaggccgc caccacgatc gcgatccggg tgcgtctcac
     9121 gcgcgccgcc gaggtgatgc acgcgcggac gaacgcctgc tcgaccggcg tgaggctggt
     9181 gtcaccgcga gcctgccagc gccggagatc cgagagcgcg tcgccgcgcc acagcagatc
     9241 cgaggcgcgc gcgcgctcgt cccagtgacg cgcggcgacc gcgagccgct cctggagctg
     9301 cgcgccatcg gcatcctcgc ggcgccacga tgccagcctc ggccaggtcg tcgccagcgt
     9361 ctcgtggatg atctcgatgc gatcgccggt gtcgtcgtcg cgcgacacga tcaggcgcgc
     9421 ggcgagcagg cgatcgatca cggcgccggc cgaggcgccg ccgagcgcgc tctcgagctc
     9481 ggtgcggctg atcagcgcgc gggcgccttc tgcggtgatc agtcggcgaa acagcaggcg
     9541 cacctggcgc cggtcgggga tcgacatccg gtcgacgacg ctgtcggcgt gctggaccag
     9601 cgcaccgatg acgccgccga tccgctgata ggcagcgcgc gtcagccggc ggccgcggcg
     9661 gtcgcggtgc tcccacagct gtgccgcggt gaaggcgacc aggggcagcg cgcccgggcg
     9721 atcggcgacc tcgtccacga tctcgcgcgg cagcgcaggg tcgtcgaacc cgaagccgca
     9781 ccgccgcgcc ggcacggtga gcatgcgctc cagatcgtcg cggcgcggga caccgagcac
     9841 gtggacggcg cgacccagga ggccccgcca cgcgggcagc tgttcgatgc ggcacaggaa
     9901 gtcgtcgcgc atcgcgagca ccacgcgcac ccgccggctc gccgatgcca tcaccagcgc
     9961 ctcggcgaag gccagccggc ccgcttgctc ggtgcacatc gtgaacagct cttctgcctg
    10021 atcgacgatc acgacaagga cgacgccgcg cgcctcggca acacccatca gacccgcggc
    10081 gagcgcagcc ggcgatgtcg cggcgccatc gcgcgcagct ttgcggtgcc cggagccgtc
    10141 gtgccgcgcc acgatcgccc acagcgcgcc gggtggatcg cctcccggac ggaccagctc
    10201 ggcgcgccag gccggtgggc ccaccgggag agcgggagcc agtcctgctg cgagaaagga
    10261 tgtcttgccg ctgcccgatg gaccgacgac ggtgatgatc ggctgggtat cgagccggtc
    10321 gatcaactcc tcgaggtcgc gctctcgccc gatgaacaac gcgtgctcgg cggcggtgta
    10381 cgcggcgagg ccgggatacg gtgacacgtc ttcgggcagg ctcgcgctcg actcctcgag
    10441 gtcgctcgtc gcgccgagcg ttgcgaacct gatcgccatc gcgagctcgg tcgggttcgc
    10501 gaaccggcgc tccggtagct tgtcgcacgc gcgagacagt gcggcgtgca gcgcggcggg
    10561 cagatggccc ggcaacgcgg ggagcgcggc gttgcgatgc tgatgggcga gtgcctcgag
    10621 cgtggtgccg gtgaaggcgt gctcgccggt cagcgcctcg taggcgacca gcgcgagcgc
    10681 gtactggtcc gcaagtggac cggccgtcgt cggatcgtgc cactgctcgg gcgccatgta
    10741 catcggcgaa ccgagcacct gatcgtcgcg cgtcaggctt cgcgacacgt cgctcgaggg
    10801 ccgcggggcc gagcccagct cgagcgccgg cgatcgcccg agtggcgcgg ctccaaaagt
    10861 gtccaccccg accagcttgg ccatgccgaa atcaagcagc ttgggcatca gcctccccgc
    10921 gcgcacgatc accatcacgt tcgacggctt gatgtcgcga tggacgatcc cctgatcgtg
    10981 cgccgactgg accacctcgc acaggcgctc gaacagcgga acgaagcgat cgaggggcag
    11041 cggtcccgac tcgcggatca gacggctcag cggcgcgccg tcgatgagct ccatcgcgat
    11101 ccacatgacg ccgtcgggct cgacgccgaa cgaatagaca tgcgccgcgt acgggtggtt
    11161 gaagcgcgat gccaggcgag cctcgcgcgt gaatcgctcg ccggcgtgcg gctgtgaggc
    11221 caacgaccga tgcaccacct tgacgacggc aggccgttcg agcgaacgct gctctgcccg
    11281 gtagacggtt ccgaagccac cggctcccac cacctcgccg agcacgaagt cgccgaggac
    11341 gcggctggtc agcggcggga gctcctcgac cgcggtgctc gcgccatccc cgtcgcgcgt
    11401 gatcgcggca cccgggcggt cgtcgaccgt cagcgcagtg acgccggtcg gagaagtcac
    11461 cgaggggaga cggccgctgg tccgttgctt gtcgtcgcgc acgctgagcc ggttcgcgcc
    11521 tcgtcgcgat cagacgtcag cagaaggccg cgcccgggac cgtgctgctc ccggtggaca
    11581 gcgggcgagg cggaggttgc cgaacacctc ccctgatgtg tggaaggtcg ccgctggaca
    11641 gctccaccag cgtcttcacg gtgtcgtgat cgagcgtgag tcgcttccgg atcgtcttct
    11701 tcataccgtc attcctcctc tgttcgctga gcaatcggcg cgcccctccg cgatcggaac
    11761 gacagccgtt cctgctgtcg cgacggagga ctccggccgt ggcgcgtcgg aacggctaca
    11821 ctctatcggt gccgtcaacg ccgcgctacg tgatacttgc attcgcagct atgacaacac
    11881 cctactcgtc ggcacacgac cggcgttcag cgcgcattcg cgagttccag acccggcggt
    11941 caccgccggt gagccgacga cgagcggcaa tgattctgta aggcggatcg ctgcaggatt
    12001 tgcgggtgct ggagcggcca agctcgcagt cgaacagcgc gcaccgagac catgcgatcg
    12061 cgctgcagcg agcacgcacc accggcggta ctgccccgag cgacgctgca gccttgggcc
    12121 gcatcgggtg gcgtaaggtg tctggattcc ggacatgcac gcctgcatcc ggacgaccac
    12181 cgagacgccg gcaggcgcgc cggatcacag cgtgcccgag ttgcccacga tcaccccggc
    12241 gtcgttgatg tcgttggcgg agctgctggt ggcgatcccg agccgcgccg gcggcacgcc
    12301 gagatcgacc atgcgcccgc cgctgtagcg gaacgcgtgc gtcgccagct cgtggtcgtg
    12361 ctctcgcacc gagcggcacg ccgatcattc gtattctgct tgtgtggacg gagtatcggc
    12421 aagcttttgt cggaagacct ctgccgtgct gcgcagatct ccgaggcgcg caggcccaca
    12481 gcttgctccg ctccaaggaa aggcccacag cctcaacagg agcttcatca tgcgtccact
    12541 caatggcttc atgacactcc aatccgctct ggtctgcgcg ctcgcgagct gctcgtccgc
    12601 ggcggccgcg cccgaggcga gcgcgggcac ctccatcgac caggccgcgc ccgacggcac
    12661 ggccacgagt gccacggctg ccgcttccgc gtacttctcg atccgcgcgg acctgcgcaa
    12721 gtgcgcgtac ccgatgtgtg gcggctggtt cctcagccgc gtcaacagtg cgacgacgca
    12781 gtgccacgac ggcacgattg cgacatcttg ctatacgccg gtgctggact ggacgaacag
    12841 cggcctggac gacacgcaac aggcggcgct gagcgaggcg gccaaccacg ccgcggtctc
    12901 gccgggcgtc tacgcgatcg tggtcggccg attcgcgcgg accaatacca cgacgccgga
    12961 tcccaccctg ggccggttcg tgatcagcca ggcgtgggtc gccgagggcg acggcgtgtc
    13021 cagcggcacg ttcgtcaagg tccaggacaa cggcctgcgc tgcttcgccg cgccgtgtcc
    13081 gaacctgacc gagaccacgc tcaacacgcc gcagaccacc gacatcgcgg aggtcgactg
    13141 gacgccgtcg gggttgaccg aggcgcagat cgaggactgc acccaggaca tgtacggtcc
    13201 cgacggcctg gtgatcgcgg gcgatcgcta cacgacgacc gtgaacggca ccgccgcgca
    13261 ggggcgcacg gtgaccgacg cgtaccagcg gctcgtcccg cagccgcagt gagcgtcagc
    13321 cgcgacctgc acagccaccc cgacctgtcc cgcgacgcct ggcacggcac cgtgatcgcc
    13381 ctgttccagc cggccgagga gacctccgag ttccacgtgg tcgaggtcga gccgacgttc
    13441 ggcgagcacc agtgcgccac cgtgttcgcg ccgcttcatg ccagcccgag cgagatcgaa
    13501 gctgctcgac ctccgcgcgc cgtgtttgat ccatatccga tgacgcagcg caaggcgtga
    13561 cccacgccgc gcgatttgtc cagttccgga ccctgcgcgt ccgtgctagg ttacacttct
    13621 tcacagcgcg ttcgactgcc cgcgacgttc tgcacggggt gatacgcagc tggggcggaa
    13681 cgccgaacgg accgattgtc tcgctggacc acgctgccca cccgcacgac cctgcaaccg
    13741 agctcctcga acgggagatc gggggacgcg tgctccgagc ctgcgcggtc ggaagcgtgc
    13801 cgccgacggc atggccgtgg gccgcgccgg cgtcacgggt cctgaaccgg gagagctaga
    13861 tagtgccaac caatcagaaa accacgtcga acaagcggca gcgcgagcag gatcagaagg
    13921 agcgcgccgc cgagcgcatc gcgcgccgca ccgaacgcaa ggcgcgggcc gccgcccgcc
    13981 tggcatcggg ccaggtcggt ccgcaaattg ccgagcctgc tccgcccgac cgtgacgtgt
    14041 cccaccacga tcccgcagcc tcgacgatgc cacccgcgat ggcaggccgc agccgccccg
    14101 agcaatcacc gacggcgcgc aggatctatg tgggcaatct gtcctacaac atggacgcga
    14161 attccatcgc gtcgctgttc gcgcagcacg gcgaggtcgc cgacgtgcac atcgtgctcg
    14221 atcaggtcag tcggcgcccg cgcggattcg cgttcgtgac catgggctcg ccggcggaag
    14281 cgcaggctgc gatcaccgag ctcgacggcc agaacgtcga cggccgcacg ctgcgcgtga
    14341 accaggcgga ggcccgcgag tccggtccga gcatgggccg cggccgttac tgaccggccg
    14401 aggtgacgct caacgagtga ccggcggtgg gctgcgcgat cgcggcgacg acgtgggcct
    14461 gtgcggcgca cgtcccagat cactccgagc ttcgagagcg cgacgagcat cgccaaggca
    14521 atgcgggtgt aggcgtcggg attcccgccg atcgccagtg agcgagtcga cccgaggcgg
    14581 atcggcgtgc ttcgcgacca ggatcatgcc tggccggtct tgtcgggccg gtcacggatg
    14641 gggagctggt gcatggactc acgcagccag cgttcgagcc tgtgcttcac ggcgtcaccg
    14701 acgctccccc agccatcgct gatgccccac ttgaggaggt gcgtgagctc ggacagcttc
    14761 gccttgacct tgtcgcgggc ctcggccgcc tcgaggcgcc tgtcctcccg gagctcgcgc
    14821 aggcgcacga acagctccgc gcgccggagc cggatgcgcg actcgagatc aggctcggag
    14881 ctcgcgcgcg ggtgtacgag ggccggctga aacttgcccg tgatcggcgg ctcgatcgcc
    14941 ggggcgggct tggcgtcgag ccgcggatcc ttgatcgtgc gaatcttcat gccctcggga
    15001 gtgcaagtca cgggccgcga gcctggtgct cgacgacgtc cagcagcagg atctcggtgc
    15061 cctcacgcgc cgtcaacgag accgaccgtt cggcggtgac acccgcgccg tccccggtcg
    15121 tcaggacgag atcgccgaac gcgacctcgc cttgcacgat gtgcaaccac acggctcgtc
    15181 cgggcgtgag cttgtgaacc acgtgctgtc ctgggtcgag cagggccgag tagatccagg
    15241 tatcgtgatg gagctgcaac gagccgctcc gcgcgtaggg cgaggcaacg agccagaggc
    15301 cgccgcgtcg ctccgcaacg ctgaaccgcc gctgttcccg cccgggctcg agcgcgaccg
    15361 cagtgcggcg cagccggatc tggaacacgt gtgcccagtc ggcacgcgat gcgttggtct
    15421 cgctgtggcg ggttccaagc gccgcgatga tgcgctggaa ctcgcccgcg tggaccgcgc
    15481 ccgagtggcc tatcgcgtcc tcgtacacga tcgtgccttc gcggacgtag gtgacgatct
    15541 cggcctgctc ggctgcgtag cccgggacat cgccgcgcgg gggaaggcga ttctcgtcga
    15601 tcgcctcgat cgttccaaag ccctctgcga ggccatcggc ctgatctcgc ggatcgaacg
    15661 tccgccagac cttcctccga cggtggggat cgtggtggcg tgcttcggct cggcggaggc
    15721 tgatcatggg tcgatcagga aacaagcagt tcccgggccg tcgaaaacat cacacgaatt
    15781 caactattta ctttccggac cgcgacacga tgcaatcaag atgatgtatc acaaccatcc
    15841 attcgaagga ggatgacgga ctcaccgcga atctcctgac gccgtttccc acggcgcgcg
    15901 gaggccgatc atcggtcgag cgatcgatct cggccttggc agcaggtgga tcgaattcat
    15961 catcaaggaa aattcgagga tgacagccca aaaccgggct tctcgttcag aatgaagtat
    16021 cctgcccatc attttgaagg aggtcgaatg gcgcgcgcgg acgagttgga ccacaaggag
    16081 ctcctggagc tcgaccccga cggcggcgtg attcgcttcg cgggtcagcg cgcgctgctg
    16141 ctcgatgccg tcgcgatcgg gctgctgcgc aagtatctcg tggagaactt cggcatcacc
    16201 gccgcacgca ccgtgctcac ccagttcggc ttcgcccacg gctggcgcat ggccgaggcc
    16261 atgcgcgccg agttcccctg ggagagcgac gacgaatggc ggagcgccgg cacacgcatt
    16321 cacgccctgg agggcctgtt tcacgtcgcg cccggtggca gcgggccgct gtccaggacc
    16381 ggatcgatga tcgtcgcctc gtacgaggcc gaacagcacc tgcttcactt cgggcgctct
    16441 gatgtgccgg tatgctggac catctgcggc ctcacgagcg gctatctgag ccggatcgcg
    16501 gagaagcaca tgtacgtgct cgagcatggc tgcgccagca agggcgacgc cgcgtgccac
    16561 ctgttcgcgc gctcgcgtga ggaatgggga ggcgagcatg ccgaggagct gcacttcttc
    16621 gagccgagcc ggctcgagga gtgcctggat gtctcgctcc agcgcgtgac gaggaccctg
    16681 caagcagccg agcgcaagct ccaccgacac cggcgagcgc tggtccaggt ggcgcgcgac
    16741 gtcgaggagc cgctcggcat cgttacccgg agcgtcacga tgcggcgggt cgtcgagctg
    16801 gcgcgccgcg tggccaaggt cgattccacc gtgctcatca ccggtgagag cggctcgggc
    16861 aaggagcgga tcgcgcgact gctccacgac gtatcgacac gcgcgaccgg accgttcatt
    16921 gcggtcaact gcggcgccat caccgagacc ttgctcgaga gcgagctgtt cgggcatgcg
    16981 cgcggtgcgt tcaccggcgc gacgcaggat cgaccggggc tgttcgaggc tgccaacagc
    17041 gggacgctgc tgctcgatga ggtcggcgag ctgtcgcccg cgatgcaggt caagctgctg
    17101 cgcgcgctcc aggagcgcga gatcagacgc gtcggcgaga acaagagccg gcatgtcgac
    17161 gtgcgcgtcg tggctgcgac gaaccgcgat ctcgcacacg gcgtcgccgg gggcgggttc
    17221 cggcaggatc tctattatcg gctcaaggtc gtcgagctgc acgtaccgcc gctccgcgag
    17281 cgcagagacg acatcctgcc gctcgcgcgc gtgctgctcg ccgatgcggc gctgcggatg
    17341 aagcgcaaga tcacgggtct ggtgcccaat gccgccgatc agctgctgcg ctatggttgg
    17401 cccggtaacg tgcgcgagct cgaaaacgtg atggagcgcg cggtcgcgct cgcgtgcgaa
    17461 agtcgggtcg agctcgagga cctgcccgag gagatccgcc acacgcttcc caggggaacc
    17521 gccggcggcg tcgtacggcc gctcgacgag gtcgagaagg agtacatcat cgcggtgctc
    17581 gcgctgaacg aaggcaatca gactcgcacc gccaagcagc tccacatcgg gtctgcgacg
    17641 ctgtaccgca agctgaaaag ctacgggctg ctcggcaagg cccggacgcg gaacagccgc
    17701 ccgcgcggcg attcgatgtg acgcgggcag gtcagcggcg gccgggcgtg gtctgcggtg
    17761 gaagcggcgg caatgacgac cagcagcgca tgcaccgcgt tgccgcgcgc attccgatct
    17821 cgccgcaggt cgggcacgtc cgtagctcgg gggatggccc gagagccagc acatctacca
    17881 gctcggccca tgcggcgcgc accttttcgg gcggtggtac gagcgcgaac tgacattcga
    17941 tcgccgtgac caggcaacgc agcgtcgcga cggatttgtc cccatagttg atcgacatgg
    18001 cgattccttt gttcactccg ccagcgactg gaccggtgaa acccagctcc gcgacgcgcg
    18061 aaactcgccg aagcgttcgc tcagcccgcg gcgggtcggc cgcgccacgc gaacgcccgc
    18121 gaacgcatgg ccgcgagcga gcgcgacgat cggtgatgat gcccatggcc gcttgctgtt
    18181 gcatgcggcg cgccacggct gtgaccgcct gatctgtcta ccatgtcgcg cccacgttcg
    18241 cgtcgcgctc ggtcgaactg atgggcaggt acttcattct gagtggacca gccgcattcg
    18301 atggacaggc aacggaactc gaagatgtcc tgcggtcccg agatcctgtt gttgacgccg
    18361 ccgagccccg agccgaagct ttgcgacgtg cgaggcggat ccaatccagc agcggctcgc
    18421 gatttgctgg gccgacggcc atgacgacca gcacgacgaa cgatgatccg cccgcgcctg
    18481 atgccaagcc cgacgcggcg gcgatcgggc cgccgattgc gcgcgagacc cagcccgagc
    18541 tcgcagtccg cgcggccgcc acggaggccg atctcgagtc gcgcatccag cagcgccgcg
    18601 gggagttgat cgcgacgctc ggtcagctca gaacggatac gcacctcgcg gcaaccgagg
    18661 tccgcggcag gctgaaggcg cagctgtccg agctcgcgca gatcatcaag gtgggcgttg
    18721 tcgacggctg gacgaacctt ggcgacaccg tgaagtacaa gctcgagcag tggctcgctg
    18781 aatccgaacg ttccatgaaa gatctgcccg ccaagaacgg gtaatcatga gccatccacg
    18841 tcacgaccgt atcctcaccc agcgcctcga cggaccggac aggatgtccg gcggcctgtt
    18901 catcgccgcg ggatgagccg gcgcgcattc gaggagccgc gaccacctct gcgtgcgcga
    18961 ccggccagac ttgctttctt cgaagacggc atgaggacac gagatatcgt tcctccgttc
    19021 catgccaccg atgacctgat cagccggcgc atggcgcaat gcggggaggt gcgcggcgtc
    19081 gacctcatcc tcgatcgcgt cactggccgt ccgcgcggct tcgcgttcgt cgagatggcg
    19141 accgccgagg gcatgcagaa agcgatcgcc gagctcgacg gcaaggactt cgaggggcgg
    19201 tcgttgaccg tcaaggtcgc cgaggagcgc ggcggtggtg gcccgcgcgg caacggtggc
    19261 ccacgccgcg gcggcgatcg cgaaggcgat cgtggctggg gccgtggccg gtagcgccag
    19321 gccggcgcgc gatgcgctcg cggatcgcgc gtcgcagatc ggcgaggcgt gggcacgcga
    19381 ctacgtgcgc gagctacgcg agcaggcacg cgcgccggtt ggtgcgtggc cgggcgcgat
    19441 gcgcgaagcg cgcatgcgcg tgaggtcgcg gcttgcaatc gtgctggagc gcgatcgaat
    19501 cgacgagctc gcgcgcgttg cgaacttcgc cgcgcgtcgc ggctggcgcg aggtgctgat
    19561 cgacggatac gaggcgaagc aggaggcgat ccgcggcggc ggctacttcg cagcggcggc
    19621 gggcggtagg catgactcgc ttcatcgcac gggcgatccg aaccacgtcg caagcgcatc
    19681 gcgcaggccg tggtccgtgc cctccccgac ccacgcgaca tgcccgtccg gccggatcaa
    19741 cacggccgtc ggagcagcga ccgcgccgag caccggcagc tcccatgcgc catcgtagcg
    19801 tgcgtcgatc tgccggactc gatccgccca tggcgtgatg tcgaaggcac caccgagatg
    19861 gagcaacacc gggcgcgcat cgtgcagcag cgtgaacacg cgcagcgggc cagcgtcggt
    19921 gaccagatcg agatcgggca tgcggcgacc gagcagtgga tgtcccgcgc cgagatcgta
    19981 gtgaatgtcg aggcccgaca tcatcgcgcc gtatcgcttg cgcggctcgt tcatccgcag
    20041 cagctcggcc atggactcgt gcaacgcctt gctgcgctcg tcgccacgat ggagcgcggt
    20101 cagcgccatc gtggtgcgga gcacgcgcgc agcaacgggg tgccgctcgg cctggtaggt
    20161 gtcgagcaag ctctcgggcg atgtcccgtg aacgacctgg gcgagcttcc atcccagatt
    20221 cacggcgtcc tggacgccgg tgttgagtcc ctgtccgccg acgggagaat gcacgtgcgc
    20281 cgcgtcgccc gccagcagca cgcgtcggtc gcggtaggac gacgcctgcc gcgctgcatc
    20341 gctgaaccgc gatagccacg tcgcgccatg agcgccgaag tccgtcccgt acgcggcgac
    20401 gagcgcctcg cggacctcgg ccagcgtcgg ctcgccgtgg ccgacgtgtg gttcttccag
    20461 cacgacgcgc acgagccttg cgtcctccac cttgcccagc gcatgggtgc ccctctcgct
    20521 gcgccggatg ccccacgccg gctcgtcggt catctcgacc tccgcgatca ggtagctcgt
    20581 cgacggctcc caccccacga agtcgatacc ggcgttcttg cggatcaggc tgcgacctcc
    20641 atcgcacccg acgagaaact tcgcccgcaa cgatcgtcca tcggacagcg cgacgtcgac
    20701 gccggtgtcg tcctgcgcga acgccgtcac ctcgcgcccg cgatagatcg gcaccgcgag
    20761 ctcgccgacc cagtcggcca ggatgcgctc gatgtggttc tgccacagcg cgagcccgta
    20821 gttgtgacgc gtcgggagat cgctgatgtc gagcgggccc gcgaagtggg cgatctgcat
    20881 ggtctgccct cgcgatagaa agcgatcggc gattccccgc tgatcgagca cttcgatcgt
    20941 gcgcgcgtgc agcccgcctg cgcgcgttcc ggcgagctcc tggctctcgc gccgctcgac
    21001 gatggcgaca ccgacccgcg ccagcgcgag ctcgcccgcc agcatcagcc ccgtcggccc
    21061 tccgccgacg atcacgaccg catgggagtc cttcatgcgt ccctgatcgc aaacctcgtc
    21121 gatcgttgga agcttgcgcc gtacggcgac tgttgagaga atgcagctgg gaagcagggc
    21181 gggatgggcc cgcctcctcg gcgtgctcgc ggatcctctt ctccccctcc agggtgaggt
    21241 cctgcgggcg gccggcgccc gcggtcggtc tcggagcctc gacggccgcc ggctcccagc
    21301 ggtcggcccc tcggcatctc cacctcgacg ccgagcttgt gccgcagctc gttctgcatc
    21361 ccgccgtcca acccaagtac gtggagatca tccccatgta cgggagtgcg gaccgccgcg
    21421 cacggcgcct accggggcgt acgcacccgg cgtcacgcag ccggcattgc gcattttacc
    21481 ggtatgcacg cttgccatga tgtttcgagg taatgtcgat ctggctctcc cggcatatag
    21541 aggttccatt ggtcgccagc accatagtaa aagcaccctc acgaggtgct gggatgaagc
    21601 ttcgcaattc gttcaattac cgaacattca cggggctgac ggcgctgctg gtcagtgccg
    21661 gcagcacggc aagtgcggag cccacgctcc gcatgcggcc aggtccacgc cggctcgccc
    21721 aggcaacgcc accggcgcag acacccccgg cgcagacgac agcgggacag tcgccaccgg
    21781 cggcgccgcc gccgtcgccg gacgcgccgc cacccccacc gacacagccg ccgccttccc
    21841 cggacgttgc gccggatggg cccgccccca cctccgatgc ggccccggct tcgaccctca
    21901 cagcgcctgc gagcccaccc gccacgcccg gaccggagga agctcccgtc gccggcgatg
    21961 acctgccgcc ggccggggcc gaggtcatcg tggtgaccgg atcgcgcatc ggcgatccgc
    22021 tgggcaagca agcgccggtg ttgatgatgt cgcgcgagga catcgagcgc acggggctga
    22081 cgtcggtcgg cgacatcctg cagcaactgc ccgtctcggg cggcgcgctc aacggcaagt
    22141 acaactcgag cggcaacttc ggatatccgc ccgacggcgg cggcatcggc gcgggcgcca
    22201 ccgaggccga cctgcgctat ctcggctcca agcgcgtgct ggtcctggtc gacggcgtgc
    22261 gctgggtcaa tggctcgtcg gccagcggcg tctccgcgtc gaccgacctc aacacgatcc
    22321 cgctcgggat catcgagcgc atcgaggtcc tcgaggacgg cgcgtcgccg ctctacggat
    22381 ccgatgcgat cgccggcgtc atcaacatca tcacgcgcaa ggagctcagc ggggtgaggg
    22441 cctcggccta taccggcgcg ttcggtcaag gcgacggggt cgtccagaag tatgacctgt
    22501 cgtggggcgg cacgaccggc aagctgtcga cggtggtcgg cggttcgttc gtcgatcagc
    22561 gcgccgtgtc gtcgggcgac cgctcgatct ccagcttccc ggtcccgaac gtggggcggt
    22621 gcacgagcaa ctgctccggg accggtgggc acggccgcgt gatctatttc gatcccaacg
    22681 gcaccaagca cgacctcgcg ctcgaggacg gcaccggggg catgttccac acgttcacga
    22741 acgatgaccg cttcaactac cagccctaca actacgccga gacgccgtcg cagcgcgtca
    22801 acatcttcag tgcactgacc tacaagctgt cgccggacat caacgtccgc ggcaaggcct
    22861 cgttcaacaa ccgcgagtcg gtcaaccagg ccgcgcccga gccgctgacc atcggtccgg
    22921 gcgcccatac caacagccgc ctggatcgca tctcggtcga cgcgaccaac ccgtacaatc
    22981 cgttcgggtt cacgttcgat cccatgaccg acaccggcta ccagatcgac cgccggccgg
    23041 tcgaggccgg accccgcatc ttccagcagg acgtccacac gttctactcg tccggcgggc
    23101 tcgatggcca tttcgacctc gggggtcagc ggttcacctg ggacaccacc gtggcctacg
    23161 gcctcaaccg cgccgagcag cgccgcaaca acgcgttcaa ctcggccaag ctccagcagg
    23221 cgctcggccc ggcgtacctg ggcagcgacg gccgctacca ctgcggcacc gcggcgaacc
    23281 cgggcgatcc caactgcgtg cccttcgacc tgttcgacgg ccagggcgcc gacggcaggg
    23341 gcaccatcac cccggcgatg ctcgcgtaca ccacgttcac cgagcacgac gtctcggagc
    23401 agaccatggt cgattccgtc gccaacctgt ccggcaacct cgtgaagctg ccggccggct
    23461 gggtcgcggc ggcggtgggc gtggagcatc gccggctgag tgggttctac gaacccgact
    23521 cggtcatcgc ggccggcgac ggcgccggcg ccgtgtcgtc gcccacctcc ggccactact
    23581 cggtcaacga ggcctacggc gagctgcgcg caccgctgct cgccggcatg accggggccc
    23641 agctgctcga cgtcgacgtc gcgggccggg tgtcggacta ctcgttcctg tcgcccaagt
    23701 tcaccggcaa ggtgagcgca cgctggaagc cgatcacgga catggtgctg cgcggcagct
    23761 tcggcagtgg attccgcgcg cccagcatcg gggagctgta tggcagcaag gcgcggttca
    23821 atccgatcct caacgacccc tgcagcgact tcaacaagtc cggggtatcc gcggccgtcc
    23881 gccagcgctg catcgatctc ggggtcccct cgaacggcag ctacgtccag gccaaccagc
    23941 agatcggcgt gaccaccggc ggcaatcgcg cgctctcgcc ggagacctcg accagcatca
    24001 acctcagcct cgcgtacagc ccggcgtggc tgcaggaccg gccgtgggtc gacagcctcg
    24061 atctcgagct cgcgtactac gacatccggc tcgactccgc ggtcgcggcg ctcgacgccc
    24121 agctgcagct caatcagtgc gtgctcaacg cggacgacaa ggcctgcatg ggcgtcgcgc
    24181 gcagcccgga gggcgcgatc tcggcgttcg acaacgtgct gcagaacatc ggcgggatca
    24241 acgtccgcgg cctcgacctg acgctggcct atcggatgcc acgccagagc ttcggccggc
    24301 tccgcgccac gctgcagtcg agctacctgc tcgcctacga gaccaagacg ccgagcgccg
    24361 gcgggttcca gaccaccgac ctggtgggta tcatatccgg aacgccaacg catgcattcc
    24421 ccaggttcaa atccaacctg gcactgtcgt ggctccacaa gacactcgac gtcacgctga
    24481 tcacccgcta tatccattcg gtgaccgagc actgcccggg actggccgcc ttcccgggca
    24541 cgtgctcgaa cccgaatccg gatgacacga agtcgaccaa caagctcggc atcaccgtgt
    24601 acaacgacgt ccaggtggtg tggtcgccgg agttcgatcg ccggctcacg gtcacggccg
    24661 gcgtgaacaa cctcctcgac cgcaatccac ccaactgctt cagctgctcg ctgaacgggt
    24721 tcgagggcac cacctacgac gtgccgggag tgttcggcta tctcaccgcg acgtaccaca
    24781 tgcagtagcg gacgtggacg tgatcgcggg ctgctcccgg ccgcagcggc aaacggtgcg
    24841 cctcgtcaga tgtagttgtt ctgcgtgggc tcgcatctcg ggtctgtcga cgagccacta
    24901 ccccccatga ccttatcgag ttgactaggt ctcaaggatg agatggttgc gacggtttca
    24961 cgacggaaga cgagcttgcc tggttgtccg gtctggttcg tggatttctt catggttcag
    25021 tccttcggtg tatccgctgt atgcatagca gtcatgtgtc gagcaacttg cgggcggctt
    25081 cgaggtccat ccagagcaga cgaccttcgc ggtcgagcgc gacagcggcc ttgccatccg
    25141 cgatcactgc gatctggctg atgtccaccg tgccggtcgg caggtagagc cagcgtcgcc
    25201 gagcaagcga gtacagccag atcgtcccat cggtacagga cgccatgacc aggtcgagcg
    25261 ccagcgcgac atggtttgcg cgcccggcca gggtcagcca ggtcggcggt tcaggacccg
    25321 atgcagcgcg acgggtgccg acatgaatgg cgccatcgtt ggtcgcgacc gcaacggtac
    25381 ggccatcgcc ggtgacggcg atgtctccca cgggcccgga gccatgcagg acgatcactt
    25441 gctgccagga cctcgtgtcg atctcgacca cgtcaccgtt tgcgtacccc gcgatcgcgg
    25501 tctgctggtc gggaagcgtg agcagccggc tgacgcgcga gcctccgatc ctgagcgaga
    25561 acacgtggcc gtcgccaccg gcgcgccaca gggcaccgtc cacggtgctg aacaggatcg
    25621 agccggccgt gcgggtggtc gcgaatcggt cgatgggagc gctggactcc acgagcaggg
    25681 tcggctgtcc ggatggcgtc cagcgcacca cccgcccatc gtgccccgac gtgatgaagt
    25741 cgtcggtgtc gtcgacgaag cgaagctgtg tgaccgagcc gtgcccggta gggatgacac
    25801 gcgcccgggt catggacgcg gcggaccaga cctcgacgag gtcgttcacg ccgtaggtcg
    25861 caaaggtcct gccgctcgcg gatcgttcga ggaagatgtt atcggacaga tggggctcga
    25921 cggctcgaac accggtcgcc ggggaaaacg tcgtgagcgc gggcaagagc gtggtcgccg
    25981 tgaccatggc cgagtgctcg tcgaagatcg cccggtagaa tggcgacctg gaggtcgcgg
    26041 cgacccgggc caggcgagcc ggcagcggcc atacccggat ggcgccgcgg acatccccgg
    26101 agatgatgaa cggctgctcg ggggtcgggg acgccagcca ggtcagccgg acgccatggc
    26161 ctcggtagga cgcgatgaga tcggtctcca gatcgagcac ggtgatgatg ccattgcccc
    26221 ctgcggcgat gacgtagtcg cccgtggcac cgtcggcgat gaaatcggca tggccctcga
    26281 gctgtgcccg gggtcgcacc gtgccgtgct ggaggtccca ggttcccagc gcaccgtcat
    26341 gacaggcata cgcgatgctc tgacgtcccg ggatgaattg cagctgggcc gtggggccgt
    26401 gacacagcgt ggttcgcgcc acgacccgcg cggggaagct gtcgagaatc accgcctcgc
    26461 cttgcttggt cgtgagcgca atctggtgct cggtcctgcc cgcggcccag cgccagaagc
    26521 ccgggagcga gtaccgctcc gacgtcccgt tcatgcgaat gaactcgatc ccgttcgggg
    26581 cgccggcgac ggcaatgtcg tcgctgatga acacgacgtc gtagccgccg gcgatcggcc
    26641 tggtcagccg gagcgcgggc cgggccgggt ccgtgacgtc gaacacgcgc aggacggcgt
    26701 cgtgggacat catgccgagc aagtttccac tgggagagaa gtccaggcca tcgatgttcg
    26761 cgtctcgcaa cggcgcagcc gtggggatga gcgcggcgcg cagggcgtcg agcaggcaca
    26821 gcgccgaggg attgcaggca tacgcgagca ggtgccggga ctgtgcatac gcccatatgc
    26881 cgctccggga cacgttgcga gcgaccacgg tggacctgcc atcgcgcgac gtccgcgcga
    26941 tcgtcccgtc gtggctgagc gagaggatct cgccattcgg aacgccctgg atccagcgta
    27001 cgttctcgct atgaggcagc gcgcgcagga ccgccacgcc gcgcccggca gcctctgcgc
    27061 ggatctgcgc cgcgcggtcg agatcggtgc cgtggtaggt cgcgagcgcg tcgaccgcgg
    27121 ccgacgggtc ggtcgccagc aggagctcgg catgcttgag cgtgaactcg tcgagcgtgg
    27181 cctgggcgga ggcccgcgca cgctcggcgg aggcccgcgc acgctcggcg cttgcctgcg
    27241 aggcatcggc gcgatcgcgc tcggtcgcga tgttgcggac gtagagcgcg gtgcccgcga
    27301 cggcaacggc ggcggccgcg gccgtcgcga gtgcgagcgc gcgatgccgc cgggtccagt
    27361 gggtgagcat cgccagcagc gagtagctgc gggctgcgat gcgcgcgccg gccttgaacg
    27421 ccttgaggtc ggcggcgagc gccccggcgt cggggtagcg gcgctcggga tccggatcga
    27481 gggccttctc ggtgatcgtg gcgaggtcct cgtcgatgcc cgcgcggcgg agcatgcggt
    27541 ggcggaggtg cgcctcgccg ggcgggatcc tctgcggcgc gcagagctcc cacagcatca
    27601 tcccgatcgc gaagacgtcg gcgcgcttgt cgacgggctc gccgcgcttc tgctcggggg
    27661 ccatgtaggc cggggtaccg aggaccgcgc ccgcgacggt gagatcatcg ccggggctgg
    27721 ggctggggct ggggcccgac ggggcgctct cgataccggc cggttcgctg ctcgacagat
    27781 ccttggcgag cccccagtcg atgacgacgg tctccccgaa ctcgccgacg acgacgttgg
    27841 cgggcttgag atcgcggtgg atgatgttgc ggccgtgcgc gtaggccatg gcgtccgcca
    27901 cggcgatgac gtgatggagc aggccgatcc ggtcctcgac ccgagctcgc tccgcgatca
    27961 gctcgcgcag ggggcgcccg gcgacgagct tcatggcgta gaacggcgtg ccttgggccc
    28021 agcggccggc gtcgtgaacg ggcacgatgc cgggatgttc gagccgcgag gtgatcagga
    28081 cttcgcgtag aaagcgcggc tcgtcggcgc tgccgcgcga cagcagctcc ttgatcgcga
    28141 cgtcgcggcc gagctctcga tcgcgaaccc gcgagacgcg accgaggccg ccgcggccat
    28201 gctcgccgaa gaagtgatag cgttgctgat cgcgaacctg gaacgggagc ggtggaggct
    28261 gatcgtcgcg cggcggagca ggcccgagag ctacggtgac atcttcatct ttgccaggct
    28321 ccattgtaac cactccacgg agctctgcgc tctcacggag cgctgcactt gccggtgccg
    28381 atcgtatcta caccagacat cgagtcacaa cccataccgg tgcagcaaga tgctccatgg
    28441 ctccggggtc ggcgcatcct gtcccgacgt ggagagctcc gccggaaaga ttcgcctcac
    28501 gcgggacctg ccagcgctgg cgatcgggac catcgtcgcc gagctgcgcg aaccccagct
    28561 tgttccaggc caggttcgcg agatcgggcg ctgccacgcg gcacgggccc gcgaccggcc
    28621 ggatgtcgag cacgaccgac atctggtacg ggctggtcgc atcgtacagg ttgtcggcga
    28681 tcagcttggc gttctggtac cggagcgcga tggctcgcga tcgctccagt ggtcgtggag
    28741 caccgcggtg ttgactcctg ccgctctggc ctcgctcgtc cccggaggga aacatgaggc
    28801 acgcggaaac aaatgcgagc gccagcgtgg ggcaagacga cagatctcat gaagcctggg
    28861 cgaggtcact gcagtgatgt gccacgcgaa ccaccgtggc aaacacgctc ggttgtttct
    28921 ctctaccgga attcgcgaca tcgcgattgc cgacagcgcc gggccgcgct cgccacacgc
    28981 ggatctcctg ccgtcgacga gcggcgccga tgccgacccg cagcgagctg ttacagtcga
    29041 ggcgccgagg ccgtagaaaa cttcagctcc tgcgttgaat cctcactcgt gtcggcgacg
    29101 tacttacaga cgtggttgta gaggcggttc caacctctgc cgtccaagga cggttgtccg
    29161 tccttgtccg gacgacgacg agtgatacgt tgcatcgcat gagtcacgga acctatctct
    29221 ggctctcggt actcgatcgc cggggccggt ttcctcctcg gacggcgacc gccttgttct
    29281 gcggctaaga ggtgcgcgtt gcttccagcg ccatcgctgc gagttggggg gcctgcgatc
    29341 atgaacctgg tcaaacacac ggcgactccg gcggtgcgcc cgcgcccggt cgcagcggtg
    29401 cgcgagctgc cgacgctggt ggtcagcgcg ctcgcgtgca gcgatcacga tgtcgagttc
    29461 gactgtcgct atcaggtggc gtggtcgatc aacccgcaca tggcgatcgg cgcggtcgac
    29521 ttcccgaccg cggcgctcca gcattccatg ttcatcgcgg cgctcgccgc cgccggcgct
    29581 cggatcgtgc cactgccgtt cgtgcacggc gcgtacgatt cagtgttcgc caaggattcg
    29641 gcgctgctgc tcgagcgccg cgggctgcgc cgcgcgttgc tcgcacgact gcgctatccc
    29701 gagcgtcagc gcgagcaagc cgcgcgggcc gacttctacg agcggtacgg ttacgaggtc
    29761 gtgtgggagg ccagcgggcc gtcgtgggaa ggcggcgaca ccgtgatgct gccgtcgggg
    29821 cacggcatgt tcctcggcta cgggccgcgt tcgagttgcg aggcggcgca gtggctcgag
    29881 cgccacgccg gcgttgccgt gacgccgctc gcgctgcgcg atcctcacct ctatcacctc
    29941 gacatggcgc tcgcggtgtt gcccgatggc accgcgctgg tctgcgagac cgcgctgacc
    30001 gcggactcga tgcacgtgct cgagcgcgcg agcgggatcc gcgaggtggt gacgatcccg
    30061 cgcgccgacg cgctcgcctt cggcctcaac ctcgtgccga tcggcgacac gatcatctgc
    30121 ggtgcgtggg tcccgcgggt cgccgcgatc atcgaggcgc gcggctatcg ctgccaggtc
    30181 gtcccgctcg atcagtttca tctcgccggt gggagcgccg cctgtctcgt cgcgaccgtg
    30241 catcgcgatc cgatcgcgcc tcgcaaacct tcaacgcccg tgcacagccg aggcaccatg
    30301 gacatctacg gaccgttgtt tcgttccgtg ctctttccgg tgtgggaacg ctacgttcgt
    30361 cagcggccgg tcgtcgagcg atggctcgag ctcaagcgga cgcagtggat gtcgctcgac
    30421 gagctcacga tgcaccagac ccgcgcgctc cagcatctga tccatcacgc gtacgcgcac
    30481 gtcccgttct accgggcgcg gttcgacgcg ctcggcctga tgcccgacga cattcgttcg
    30541 ccggcggacc tgttgcagtt accggtgatc cggcgcagcg atctgcaatc gcgcagcatc
    30601 gagcgcgcct cgcgcgtgcc gccgctgccg acgatccgca agcagaccag cggcaccacg
    30661 ggcgagccgc tgctgttcgc gttcgagccc aacagcgagc actggcgtcg cgcggtcaag
    30721 ctgcgcggct acgagtgggc gggctatcgc ccgggagatc gcgcgttgca cttctggggc
    30781 gcgccgcacc cgagcgagcc gccgtggagc acgcggatca agatcgcgct cgatcgcaag
    30841 atgaaccgcg acatctacat cccgtgtgcg gtgatgagcg aggaccggtt gcgcgatgtc
    30901 gtgcgcgtga tcgagcgcgt gaagccgcag gtcctggtct gctacgcgca ggccggtgcg
    30961 gagctcgcgc gctacatcca tcgcaacggg ctgcgttcgt ggcagacgat cccggtgatc
    31021 tgtggcgcgg agcgactcct gccgcgcgat cgcgccgacc tcgaggacgc attcggcccg
    31081 tcggtgttcg acacctacgg gtgccgcgag gtgatgatga tcgcctccga gtgcgaggcg
    31141 caccacggcc tgcacatcgc gatggagaac ctggtcgtcg agatcgtcgt gaccgaggac
    31201 ggccgccagc gcctcgcccg cgagggcgag agcggggagg tcgtgttcac cgatctgcac
    31261 aactacggga tgccgttcat ccgctacgcg aatggcgaca tcgcgaccgc ggggcagacc
    31321 cggcggtgct cgtgcggacg cacgctgccg cgcatccagt cgatccaggg ccgcatgtcc
    31381 gagacgttgc gcgacggaag cggcgcaccg gtcagcggca tcgcgctcag cttcctgttc
    31441 caggacatct ccagtgcggt ccgccagttc caggcgatgc agcacaagga tcggtcggtg
    31501 acgatcagcg tcgtgcagtc ggagccacta tcgtcgcaga tgatcgagga cgttcgccgc
    31561 aacggccagc gcctgctggc cggcgtcgac gttcgcgtcg tcgtcgtccc cgagttgccg
    31621 cgcaacccgg cgggcaagca caacctggtg gtcgtcgagc gctagctacg gaagctcttt
    31681 cgtcttgccc ttctcgaacg agtacctggg cacctggatc cacgcgccgt cctcctgctt
    31741 cggctccttg tggatgcgca cctcggtgac ctccagcgtg ccgttcttgt cggcgagcca
    31801 gaagtcgatg tcgtaatact tgttcgcgct cttgtgctcg cggaagtcgg tgcacgcgaa
    31861 gtagcccttg ccctccatct ttcgcatcgg gcgatggatc tcgacgaagt tcagcgtgcg
    31921 ctgctcgccg gtcttgtcgt ccttgaccgt gaagacgccg tccttggcct gctttgcgat
    31981 gtatccgttg atcgcggcct cgatctgcca gccgcgcttc tcctcgaact cgcccgggtg
    32041 ctcgctggcg ggaatccacc accagagcag cgggttgcgc gtgaccagcg tccacttgtc
    32101 gccgtcccgc ttgggcgcct tgtggatgcg gacgtcgacg acatcgagcg agtcttcctt
    32161 gagccagaag tcgatgtcgt acggcttctt ggcatcggcc ttggtgtgga actgcgcgca
    32221 gacgaagaac ccgtagccgt ggatgccgcg cgccacccgg atctgctccg gcacgagctc
    32281 cagctgcgcg tccgcttgcg cgtccttgaa ccggaagacg ccttccttct tgaccctggc
    32341 atcgaggtac ttctgcacca cggcactggc gtcgtccggc gtgtgctcct cgtccgccca
    32401 gcctgcgacg ggcaggagac tcacgatgcc gagtgccagc agcacgcttc tcttcataca
    32461 gctcatgcga gttcctccgg cgatcgcccg atgagacagt tcgggcctgg tcggccgcga
    32521 ggatacaccc gggcgacctc cggcggtcag cgcccgtacc ggttcgccgc cagattcgcg
    32581 gcggtcgcaa gctggatatc cttgaaggtg ctggaaggtg ctggcaaagt tcgaggtgac
    32641 gccgatcgcg ccgccgagca ccggctacgg tcataggcga tgcacgacga cgatcaacgg
    32701 tgtgggtaca cgatcgaatg cggcgatcgt gtggcggcga gtcctcgggt cagcgtggtg
    32761 atcccggcgt tccaatcgcg atggctcgat caggcgctcg acagcgtgcg cgcccagacg
    32821 ttcccgagct acgagatcgt ggtggtcgac gacggctcgc cggtgccgct ccagccggcg
    32881 aagcgcgacg acgtcgtggt ggtgcgccag accaacaccg gcccgggcgg ggcgcgcaac
    32941 cgcggcgtcg ccgtcgcgcg cggcgagctg atcgcgttcc tcgactccga cgatcgctgg
    33001 cgaccgcaga tgctcgagcg ccaggtcgcg tggatggatg ccaacccgac cgcggtgctg
    33061 gtgttcaccg gcgtccacct cgacggccgg aacccgcgcg acgagagcta cgcccacctg
    33121 ctcgatccgc gcagcggtcg gctgccgtac ggcgtgctgt tcgaggagaa catgatcccg
    33181 accagctgcg cgatgatccg gcgccaggcc tacctgcgca cgccggggat gagcttgcac
    33241 cgccggctcg gtgaggacta cgcgctatgg ctgcggatcg cgctgctcgg cgaggtcggc
    33301 ttcatcgacg aggtcctgct cgaccgccgg gtgcacaccg ggagcctgat ggcgggggcg
    33361 agccgcgacg cgacgatcga ggccgatctc gagatctacg aggagctgtt cgccgagcat
    33421 cccgagctgc gtggggcgtt cgccccgcgc gcgctcgcgc gcgtcgagtt ccagcgcggc
    33481 tacgagtgcc tcgccacggc gcggtgggcc ggcgcgcggc aggcgttcgc gcggtcgctg
    33541 g
//
LOCUS       DASVOR010001206         4393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_450409,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001206 DASVOR010000000
VERSION     DASVOR010001206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4393
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450409"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..1557)
                     /locus_tag="VF516_22955"
     CDS             complement(178..1557)
                     /locus_tag="VF516_22955"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEX8110617.1"
                     /translation="MHISVFDIFKIGIGPSSSHTVGPMRAARRFAERLDADGELARTA
                     SVKVELFGSLGHTGKGHGSDKAVLLGLEGEEPATVDVDSVAPRVAAQAAAKQVRLLGK
                     HPVALDPEAQLVFHRRDKLPLHANGMRFTAYGADGGVIAERIYYSVGGGFVVDHAGAP
                     ADGSVPAERTQVPYPFNTAAELLQQSVEHGKSISGLMLDNEAALRPEAEVRRGVLAIW
                     NAMEACVKRGCEREGILPGGLKVRRRAAALHRKLKSDPAGASDPLMIMDWVNLFALAV
                     NEENAAGGRVVTAPTNGAAGIVPAVLMYYRRFLPNADDEGTIRFLLTAAAIGALYKKN
                     ASISGAEVGCQGEVGVACSMAAGALAEVMGGTAAQVENAAEIGMEHNLGLTCDPIGGL
                     VQVPCIERNAMGSVKAINAARLALQGDGTHKVSLDKVIRTMWRTGADMSSKYKETARG
                     GLAVNIIEC"
     gene            complement(1616..2842)
                     /gene="serA"
                     /locus_tag="VF516_22960"
     CDS             complement(1616..2842)
                     /gene="serA"
                     /locus_tag="VF516_22960"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEX8110618.1"
                     /translation="MAETSFPKPEIKILLLENIHPVAAEALRAEGFQVETARAALPEA
                     ELVQRVRDVHVLGIRSKTQVTPAVLAEAGRMLAVGAFCIGTNQIALGAANRRGVPAFN
                     APFSNTRSVAELILAEVVMLSRHLGDRSREVHDGRWRKVASGSHEVRGRTIGIVGYGH
                     IGSQVGVLAEAFGMRVVFFDIAAKLPMGNNRALPRLDDVLEAADFVTLHVPETPQTRN
                     LIGPAELARMKPGACLLNASRGSVVVIDALADAIRRGHLGGAAIDVYPEEPEGNSDGF
                     ASPLRGLPNAILTPHIGGSTEEAQEAIGREVSAALIKYINTGATTGAVNFPRVELPLD
                     RDAHRILNVHKNVPGVLRDINRIVSDCNANIRAQVLSTDVDIGYLVMDLDQDVSQDVK
                     RGIAALPTSIKTRILF"
     gene            complement(2886..3770)
                     /locus_tag="VF516_22965"
     CDS             complement(2886..3770)
                     /locus_tag="VF516_22965"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX8110619.1"
                     /translation="MKWTPGTENDDVIDERGGGGGGGGGGFGAMHLTGGGLATVVVIV
                     VVARLFGVDVSGLFSHSSAPVATRRPDQPHPPTGPDADKELVDFVKFAMKDIQHVFET
                     RFQAEGKPYRHAKLVLFTEGRNTGCGWSTSAIGPFYCPADEHAYIDLSFYRELKQRFG
                     APGNFAQAYVLAHEMGHHLQKLLGVEQRAQTLGRGHSRNDISVRTELQADCFAGVWGH
                     DARGEGLLEAGDIESALTAATAIGDDRLQKQAGREVNPETFTHGTSAQRVRWFRTGFD
                     TGRFDACNTFAARDSRDL"
     gene            3797..>4393
                     /locus_tag="VF516_22970"
     CDS             3797..>4393
                     /locus_tag="VF516_22970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110620.1"
                     /translation="MSPRAKPAAKDAKPATPRKRKKKAEAGSVGLAAGEVAAGSPPGT
                     LTPLRARIEATGGAVLTTYREPFGGNWVTLAALPLDRVKPTPYQRELSKAHTDRLAAV
                     IPKVGRFLDPVIAVADPDGDGFISPNGMHRLAALGALGAKTVIALVIPEPEIAFRILA
                     LNTEKAHNLKERSLEVIRMYRGMVGAGRAGVESDDIALF"
BASE COUNT          588 a         1610 c         1527 g          668 t
ORIGIN      
        1 ctcggtgacc ccggcggcgc cgagcgcatc ccacagcgcg cgctgggccg cggcgtcgag
       61 cggccccgca ctccgcgcga tcacgatacc gcgcgcgagg tcgctccggc gcggctcgag
      121 cccgacgatc tccactggat ccagcctacc tcacatcgcg acgcagggaa ccagccgtca
      181 gcactcgatg atgttcaccg cgaggccgcc gcgcgcggtc tccttgtact tgctactcat
      241 gtccgcgccg gttcgccaca tcgtcctgat gaccttgtcg agcgagacct tgtgcgtgcc
      301 gtcgccctgc agcgcgagcc gcgcggcgtt gatcgccttc accgagccca tcgcgttgcg
      361 ctcgatgcac ggcacctgga ccagcccgcc gatcggatcg caggtcagcc cgaggttgtg
      421 ctccatgccg atctcggcgg cgttctcgac ctgcgccgcg gtcccgccca tgacctcggc
      481 cagcgcgccg gccgccatcg agcacgcgac cccgacctcg ccctgacagc cgacctcggc
      541 cccgctgatc gatgcgttct tcttgtacag cgcgccgatc gcggcggcgg tgagcaggaa
      601 ccggatcgtg ccctcgtcgt cggcgttggg caggaaccgc cggtagtaca tcagcacggc
      661 cgggacgatg cccgccgccc cgttggtcgg cgcggtcacc acccggccgc cggccgcgtt
      721 ctcctcgttg accgcgagcg cgaacaggtt gacccagtcc atgatcatca gcggatcgct
      781 cgcgccggcc ggatcgctct tgagctttcg gtgcagcgcc gccgcgcggc ggcggacctt
      841 gagccccccg ggcaggatgc cctcgcgctc gcagccgcgc ttgacgcagg cctccatcgc
      901 gttccagatc gcgagcacgc cgcgccggac ctcggcctcg gggcgcagcg cggcctcgtt
      961 gtcgagcatc aggcccgaga tgctcttgcc gtgctcgacg ctttgctgca gcagctcggc
     1021 cgcggtgttg aacgggtacg gcacctgggt gcgctcggcc ggcacgctgc cgtcggccgg
     1081 cgccccggcg tggtcgacga cgaacccgcc gccgaccgag tagtagatcc gctcggcgat
     1141 caccccgccg tcggcgccat acgcggtgaa ccgcatgccg ttggcgtgca gcggcagctt
     1201 gtcccggcga tggaacacca gctgggcctc ggggtcgagc gcgaccgggt gcttgccgag
     1261 caggcggacc tgcttggccg cggcctgtgc ggcgacccgg ggcgcgacgc tgtcgacgtc
     1321 gacggtcgcc ggctcctcgc cctcgaggcc gagcagcacc gccttgtcgc tgccgtggcc
     1381 cttgccggta tgccccaggc tgccgaacag ctcgaccttg acgctcgcgg tccgcgccag
     1441 ctcgccgtcg gcgtcgagcc gctcggcgaa ccggcgcgcc gcgcgcatcg ggccgaccgt
     1501 gtgcgagctc gacgggccga tcccgatctt gaagatgtcg aacacggaga tgtgcacgtc
     1561 gcctcgattc tacccgccga ttgtacccgc caatttcggc cgccggtgca cgcggtcaga
     1621 acaggatccg ggtcttgatg ctggtcggca gcgcggcgat cccgcgcttg acgtcctgcg
     1681 agacatcctg atcgaggtcc atcaccaggt agccgatgtc gacgtcggtc gacagcacct
     1741 gggcccggat gttggcgttg cagtccgaca cgatccggtt gatgtcgcgc agcacgcccg
     1801 gcacgttctt gtggacgttg aggatccggt gcgcgtcccg gtcgagcggc agctcgacgc
     1861 gcggaaagtt gaccgcgccg gtggtcgcgc cggtgttgat gtacttgatc agcgcggccg
     1921 acacctcgcg gccgatcgcc tcctgggcct cctcggtcga gccgccgatg tgcggcgtga
     1981 ggatcgcgtt gggcagcccg cgcagcggcg aggcgaaccc gtcgctgttg ccctccggct
     2041 cctcggggta gacgtcgatc gccgcgccgc cgaggtggcc gcgccggatc gcatcggcca
     2101 gcgcgtcgat caccaccacg ctgccgcggc tggcgttgag caggcacgcg ccgggcttca
     2161 tccgggcgag ctcggcgggg ccgatcaggt tcctggtctg cggggtctcc ggcacgtgca
     2221 gggtgacgaa gtccgcggcc tccagcacgt cgtcgagccg gggcagcgcg cggttgttgc
     2281 ccatcggcag cttcgcggcg atgtcgaaga acacgacccg catgccgaat gcctcggcga
     2341 gcacgccgac ctgactgccg atgtggccgt agccgacgat gccgatcgtc cggccgcgga
     2401 cctcgtggct gccggaggcg accttgcgcc agcggccgtc gtggacctcg cgcgagcggt
     2461 cgccgaggtg gcgcgacagc atcacgacct cggcgaggat cagctcggcg accgagcgcg
     2521 tgttcgagaa cggcgcgttg aacgccggga cgccgcgccg gttggcggcg ccgagcgcga
     2581 tctggttggt gccgatgcag aacgcgccga ccgcgagcat gcggccggcc tcggcgagca
     2641 ccgccggcgt cacctgggtc ttgctgcgga tcccgagcac gtggacgtcg cgcacccgct
     2701 ggacgagctc tgcctcgggc agcgcggccc tggcggtctc gacctggaag ccctcggcgc
     2761 ggagcgcctc ggccgccacc gggtggatgt tctccagcag caggatcttg atctcgggct
     2821 tggggaacga cgtctcggcc acgggctgcg tcatacctcg catctcgccc ggcgcacagc
     2881 ggccctcaca gatcgcggga atcgcgcgcg gcgaacgtgt tgcacgcgtc gaaccgcccg
     2941 gtgtcgaatc cggtgcggaa ccagcgcacg cgctgcgccg aggtgccgtg ggtgaacgtc
     3001 tcggggttga cctcgcgtcc ggcctgcttc tggagccgat cgtcgccgat cgcggtcgcc
     3061 gcggtgagcg cgctctcgat gtcgccggcc tcgagcaggc cctcgcccct ggcgtcgtgg
     3121 ccccacacgc cggcgaagca gtcggcctgg agctcggtgc gcaccgagat gtcgttgcgg
     3181 ctgtggccgc ggccgagcgt ctgggcccgc tgctcgacgc cgagcagctt ctgcaggtgg
     3241 tgccccatct cgtgggcgag cacgtaggcc tgtgcgaagt tgcccggtgc gccgaaccgc
     3301 tgcttgagct cgcgatagaa cgacaggtcg atgtaggcgt gctcgtcggc cgggcagtag
     3361 aacgggccga tcgcgctcgt cgaccagccg catccggtat ttcttccctc ggtgaacagc
     3421 accagcttgg cgtggcggta cggcttgccc tcggcctgga accgggtctc gaacacgtgc
     3481 tggatgtcct tcatcgcgaa cttgacgaag tcgaccagct ccttgtccgc gtccgggccg
     3541 gtcggcggat gcggctggtc cggccgccgg gtcgcgaccg gcgcgctgct gtggctgaac
     3601 aggccgctga cgtcgacgcc gaacagccgc gcgacgacca cgatgacgac cacggtggcc
     3661 aggccgccgc cggtcaggtg catcgcgccg aagcccccgc ctcctcctcc accgccgccg
     3721 ccccgctcgt cgatgacatc gtcgttctcg gtgccaggag tccacttcac agcagcaagg
     3781 gtactacagt gacgccatgt cgcctcgtgc caagcccgct gccaaggacg ccaagccggc
     3841 cacgccgcgc aagcggaaga agaaggcaga ggccggctcg gtcggcctgg ccgccggcga
     3901 ggtcgcggcc gggtcgccgc cggggacgct caccccgctg cgcgcccgga tcgaggccac
     3961 cggcggcgcc gtgctcacca cctaccgcga gccgttcggc ggcaactggg tgacgctcgc
     4021 ggcgctgccg ctcgaccggg tcaagccgac cccgtaccag cgcgagctgt ccaaggccca
     4081 caccgatcgg ctcgccgcgg tgatccccaa ggtcggccgg ttcctcgacc cggtgatcgc
     4141 cgtggccgac cccgacgggg acggcttcat ctcgcccaac ggcatgcacc gcctcgccgc
     4201 gctcggtgcg ctcggcgcca agaccgtgat cgcgctggtc atccccgagc cggagatcgc
     4261 gttccgcatc ctcgcgctca acaccgagaa ggcccacaac ctgaaggagc gctcgctgga
     4321 ggtaatccgg atgtaccggg gcatggtcgg cgccgggcgc gcgggcgtcg agagcgacga
     4381 catcgcgctc ttc
//
LOCUS       DASVOR010001207         2773 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_304549,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001207 DASVOR010000000
VERSION     DASVOR010001207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304549"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF516_22975"
     CDS             complement(<1..318)
                     /locus_tag="VF516_22975"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkene reductase"
                     /protein_id="HEX8110621.1"
                     /translation="MTDLFDPIQLGELALPNRIVMAPMTRNRAVGTLPGERNAIYYAQ
                     RATAGLIISEATQVTPRGVGYPDTPGIHDDAQVAGWRLVTDAVHRAGGRIALQLWHVG
                     RISH"
     gene            complement(315..1352)
                     /locus_tag="VF516_22980"
     CDS             complement(315..1352)
                     /locus_tag="VF516_22980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8110622.1"
                     /translation="MRFGYWMPVFGGWLRNVDDERMDATFAYNKKLARRSEQIGFDLT
                     LIAELFLNDIKGISAPCLDAWSTAAALAAVTESLELMVAVRPTFHQPAIFAKQAANVD
                     RIANGRLSLNVVSSWWSDEARRYGVHFEQHDDRYGRTAEWLTIVNGAWSEPTFSYTGK
                     HYKVEDVILEPKPVRRPRPVIYAGGESPAAKALISSQCDAYVMHGDAPARVGEKVADM
                     EQRRAKLGLPRMIYGVAGYTIVRNDQAEVDRELARITNVAPDSPGYHNYQDWLANTQL
                     ENRVTLEEYSVSNRGLRSGLCGSPWQVQDQVVAFEKAGIDLLLLQCSPQLEEMERFAE
                     TVIAPLRTRAA"
     gene            complement(1354..2319)
                     /locus_tag="VF516_22985"
     CDS             complement(1354..2319)
                     /locus_tag="VF516_22985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110623.1"
                     /translation="MKSRQPIGIFYEHPDWFRPLFATLERRGYPFARIDAAGHAYDPS
                     ERTAPYALVFNRASPSSYTRGRRQVTFHTLQWLRHLERIGVPVVNGSKVYTMEISKAA
                     QLEILEELRIPYPASRVVNHPSQVPGAAQGLRFPLLVKANIGGSGAGITRFEDADQLA
                     AAVRAGRLEFGVDDTALVQEAAPLRGGAITRVEVLGGEILYAIRVFPEVGSFNLCPAD
                     ACQTAGGQELTRAACALDAPKNGMRVEAYDAPREIKDAVLAIAARTGLDIGGVEYLID
                     DRDGKPYVYDINALSNFVADGPRVVGFDPFERLVDYLARRAWGEA"
     gene            2452..>2773
                     /locus_tag="VF516_22990"
     CDS             2452..>2773
                     /locus_tag="VF516_22990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8110624.1"
                     /translation="MADEADDRDLDLDLDLDLDLVLARRGDRDAFGRLVRRHQRRVYA
                     AALHILGNHSDADDVTQEAFVRAYRGLAAFDGRADFFTWLYRIAVNTAINALRSGKRG
                     AALHQ"
BASE COUNT          389 a          951 c         1001 g          432 t
ORIGIN      
        1 gtgcgagatc cggccgacgt gccacagctg cagcgcgatc cggccgccgg cgcggtgcac
       61 ggcgtcggtg acgagccgcc agcccgcgac ctgcgcgtcg tcgtggatgc cgggcgtgtc
      121 ggggtagccg acgccgcgcg gcgtcacctg ggtggcctcg ctgatgatca gtccggcggt
      181 cgcgcgctgc gcgtagtaga tcgcgttgcg ctcgccgggc agcgtgccga ccgcgcggtt
      241 gcgcgtcatc ggcgccatca cgatgcggtt gggcagggcg agctcgccga gctggatcgg
      301 gtcgaacagg tcggtcatgc ggcccgcgtg cgcagcgggg cgatcacggt ctcggcgaac
      361 cgctccatct cctcgagctg cgggctgcac tgcagcagca gcaggtcgat gccggccttc
      421 tcgaacgcga cgacctggtc ctggacctgc cacggcgagc cgcacagccc cgagcgcagc
      481 ccgcggttgg acaccgagta ctcctcgagg gtgacgcggt tctcgagctg ggtgttggcg
      541 agccagtcct ggtagttgtg gtagcccggc gagtcgggcg cgacgttggt gatccgcgcg
      601 agctcgcggt cgacctcggc ctggtcgttg cggacgatcg tgtagccggc gacgccgtag
      661 atcatgcggg gcaggccgag cttggcgcgg cgctgctcca tgtcggcgac cttctcgccg
      721 acccgggccg gcgcgtcgcc gtgcatgacg taggcgtcgc actgcgacga gatcagcgcc
      781 ttggccgccg gggactcgcc gccggcgtag atcaccgggc gcggcctcct caccggcttg
      841 ggctcgagga tcacgtcctc gaccttgtag tgcttgccgg tgtaactgaa tgtcggctcg
      901 ctccacgcgc cgttgacgat cgtcagccat tcggcggtcc ggccgtagcg gtcgtcgtgc
      961 tgctcgaagt gcacgccgta gcggcgggcc tcgtcggacc accacgacga cacgacgttg
     1021 agcgacagcc ggccgttggc gatccggtcg acgttggccg cctgcttggc gaagatcgcc
     1081 ggctggtgga acgtcgggcg caccgcgacc atcagctcga gcgactcggt gaccgccgcg
     1141 agcgcggccg cggtcgacca cgcgtcgaga cacggcgccg agatcccctt gatgtcgttg
     1201 aggaacagct cggcgatcag cgtcaggtcg aacccgatct gctcgctgcg ccgggccagc
     1261 ttcttgttgt aggcgaacgt ggcgtccatg cgctcgtcgt cgacgttgcg cagccagccg
     1321 ccgaacacgg gcatccagta tccgaaccgc atctcaggcc tccccccacg cgcggcgcgc
     1381 gaggtaatcg accagccgct cgaacgggtc gaacccgacc acgcggggcc cgtcggcgac
     1441 gaagttggac agcgcgttga tgtcgtagac gtagggcttg ccgtcgcggt cgtcgatcag
     1501 gtactcgacg ccgccgatgt cgagcccggt gcgcgccgcg atcgcgagca ccgcgtcctt
     1561 gatctcgcgc ggcgcgtcgt aggcctcgac ccgcatgccg ttcttgggcg cgtcgagcgc
     1621 gcacgcggcg cgggtcagct cctggccgcc cgcggtctgg cacgcgtcgg ccgggcacag
     1681 gttgaacgag ccgacctcgg ggaacacccg gatcgcgtag aggatctcgc cgccgagcac
     1741 ctcgacgcgc gtgatcgcgc cgccgcgcag cggggcggcc tcctggacca gcgcggtgtc
     1801 gtcgacgccg aactcgagcc ggccggcgcg gaccgcggcc gccagctggt cggcgtcctc
     1861 gaaccgcgtg atgcccgcgc cgctgccgcc gatgttggcc ttgaccagca gcgggaaccg
     1921 caggccctgg gcggcgccgg gcacctgcga cggatggttg acgacccggc tcgccgggta
     1981 ggggatgcgg agctcctcga ggatctcgag ctgcgccgcc ttcgagatct ccatcgtgta
     2041 gaccttgctg ccgttgacca ccggcacgcc gatccgctcg aggtggcgca gccactgcag
     2101 cgtgtggaac gtgacctggc gccggccgcg ggtgtacgac gatgggctcg cgcggttgaa
     2161 caccagcgcg tacggcgcgg tgcgctccga aggatcgtag gcgtggccgg cggcgtcgat
     2221 ccgcgcgaac ggatagccgc ggcgctcgag cgtcgcaaac agcggccgga accagtctgg
     2281 gtgctcgtag aagatgccga tcggctgcct ggacttcatg atttcagagg tttatccgcg
     2341 ataccggacg aatgcaatac ggtttgatga ggtccgtgtg tgcgatcgcc gcatgaccca
     2401 acgcggcgtg cgcggcgatc gtccaatccg ggtcgcatat agtccccggg gatggctgac
     2461 gaggcggatg accgcgatct cgacctcgat ctggacctcg atctggacct cgttctcgca
     2521 cggcggggag accgcgacgc cttcggccgc ctggtgcggc gccatcaacg acgcgtgtac
     2581 gcagcagcgc tccatatcct cggcaaccac agcgacgcgg acgacgtcac ccaggaggcg
     2641 ttcgtgcggg cgtaccgcgg gctggcggcg ttcgacggcc gcgccgactt cttcacctgg
     2701 ctgtaccgga tcgcggtgaa caccgcgatc aacgcgctgc gctccggcaa gcgcggcgcg
     2761 gcgctgcacc agc
//
LOCUS       DASVOR010001208         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420684,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001208 DASVOR010000000
VERSION     DASVOR010001208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420684"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VF516_22995"
     CDS             <1..1213
                     /locus_tag="VF516_22995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110625.1"
                     /translation="AAGELYGVLDVGYAPNADARVGDEPRVQAFAAHLAIALRTLRLA
                     ADALGLRDYQARLLENASALILGVDRGWRITACNRALLELTGMRRDRLLGRDIRDFLT
                     PDQRQYLTSVFAAAMIGQHHAAVTVALPTRHHGTVPTVWSVAPVGRVGATGGPIEAVV
                     AIGQDMSPIEALQQQVIRAERLATLGQLAAGVVHELNNPLTSITVYAEYLVRKLAAQG
                     AEEADLEKLRRIGASAERILRFSRDLVQYARPSGKDVEPVDLAAVVRQSVSICEHLVE
                     RGGITLTVEVDPELPVVRAVRGQLEQVLINLITNAVHAVENGGQVRVRAQVDSPSTIL
                     LEVADSGPGVPDEDRDKIFEPFFTTKSDGKGTGLGLPIVRNIVEQHRGEIRVERSDLG
                     GATFRVILPIM"
     gene            1492..1722
                     /locus_tag="VF516_23000"
     CDS             1492..1722
                     /locus_tag="VF516_23000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110626.1"
                     /translation="MKGDYRIIVDKRASAEERVTTLATALAQVMKQNGISPEDTALEL
                     PPKVREVLRMHEPGERDPARPAGRAAGGRTAA"
     gene            1769..2431
                     /locus_tag="VF516_23005"
     CDS             1769..2431
                     /locus_tag="VF516_23005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110627.1"
                     /translation="MLRRTAALLVAGIGASVGLAACSHSKPARNLDLDLIRVSPDARL
                     RTDTVGDGKFASTASFVLVDAENTAAEGAYVTLGGELTDPGGATVGELGSQSLWIPPH
                     EARTFALVDTERLARPTAGAARIKVRGALVPDEPPRAHISDLHSFDDHGKVVVQAYLV
                     NVADRPGQIMAIGSFHDAHGQPMTRPFQLLEVGARDRRVVQFVGPEGSTRGTIFVGDT
                     VY"
     gene            complement(2492..2626)
                     /locus_tag="VF516_23010"
     CDS             complement(2492..2626)
                     /locus_tag="VF516_23010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110628.1"
                     /translation="MDDVPFDLRHRRALMYEYTPRGCKKLEKDLYENVQNMLGRPSEA
                     "
     gene            complement(2656..3144)
                     /locus_tag="VF516_23015"
     CDS             complement(2656..3144)
                     /locus_tag="VF516_23015"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8110629.1"
                     /translation="MLQLDVFLSASSKDREFAQRLAKDLVAAGLRVWNQWDTTVGDSF
                     AGAIEKGIRASRFMLIVMSPDYFQSAWTQQEWQYGLAEEVKVDRIRLVPILYRDCEIP
                     PMLRTKQWADFRDGDQYRLALAHLIRDLRSLSGAEPAAQPATEAPRSGEHVEQMDALH
                     MR"
     gene            complement(3154..>3498)
                     /locus_tag="VF516_23020"
     CDS             complement(3154..>3498)
                     /locus_tag="VF516_23020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110630.1"
                     /translation="PALPDIASSRELEVLPGFRRAPSRRELTVPPAASEFPALFEPAA
                     GTAKRAGKSRPAKSTQKTRPQPKPAETSAAPSADVDTAPAPRSRNLVWLILLMVALGA
                     GAMAAAYYLPDL"
BASE COUNT          528 a         1268 c         1184 g          518 t
ORIGIN      
        1 ggccgccggc gagctctacg gcgtgctcga cgtcggctac gcgccgaacg ccgacgcgcg
       61 cgtcggcgac gagccccggg tccaggcgtt cgccgcccac ctcgcgatcg ccctgcgcac
      121 gctgcggctc gccgccgacg cgctcggcct gcgcgactac caggcccgcc tgctcgagaa
      181 cgccagcgcc ctgatcctcg gggtcgaccg cggctggcgg atcaccgcgt gcaaccgcgc
      241 cctgctcgag ctcaccggca tgcggcgcga ccgcttgctc ggccgcgata tccgcgattt
      301 cctgaccccc gaccagcgcc agtacctgac ctccgtgttc gccgccgcga tgatcggcca
      361 gcaccacgcc gccgtcaccg tggcgctgcc gacccggcat cacggcaccg tgcccaccgt
      421 gtggagcgtc gccccggtcg gccgggtcgg cgccaccggc gggccgatcg aggccgtcgt
      481 cgcgatcggc caggacatgt cgccgatcga ggcgctccag cagcaggtga tccgcgccga
      541 gcggctcgcc accctgggcc agctcgccgc cggcgtcgtc cacgagctca acaacccgct
      601 gacctccatc acggtctacg ccgagtacct ggtgcgcaag ctcgccgccc agggcgccga
      661 ggaggcggac ctcgagaagc tgcgccggat cggcgccagc gccgagcgca tcctgcggtt
      721 ctcgcgcgac ctggtgcagt atgcccggcc gtcgggcaag gacgtcgagc cggtcgatct
      781 cgccgcggtg gtccgccagt cggtgtcgat ctgcgagcac ctggtcgagc gcggcggcat
      841 caccctcacc gtcgaggtcg atcccgagct gccggtggtc cgggcggtcc gcggccagct
      901 cgagcaggtc ctgatcaacc tgatcaccaa cgccgtccac gcggtcgaga acggcggcca
      961 ggtccgggtc cgggcccagg tcgacagccc gtcgaccatc ctgctcgagg tcgccgactc
     1021 gggccccggg gtccccgacg aggaccgcga caagatcttc gagccgttct tcaccaccaa
     1081 gtccgacggc aagggcaccg gcctgggcct gccgatcgtc cgcaacatcg tcgagcagca
     1141 ccgcggcgag atccgcgtcg agcgatccga tctcggcggt gccacgttcc gcgtcatcct
     1201 tcccatcatg tagatctccc cgagcgatcc gggcgtcctc ggggcgggcc gatccccggg
     1261 cggccgatcc cgtgagacag gcgcacaccg tgcgccgaga gatctcctcc gaggaggatt
     1321 cgtccggtac gcggacacaa aggttgcgcc cgcgatcgat cttggtactg tgggttggcc
     1381 atgaagcctg aagtcgtgct cgagctcctc gaggccgccg ccgagaagct caaggttcgc
     1441 gtcagctaag aaccgctcca gaccagcgtc gtgcacgggg gcctgtgccg cgtgaagggg
     1501 gactaccgga tcatcgtcga caagcgcgcc tcggcggagg agcgcgtcac cacgctcgcc
     1561 accgcgctcg cccaggtgat gaagcagaac gggatctccc ccgaggacac cgccctggag
     1621 ctacccccca aggtgcggga agtgctgcgg atgcacgagc cgggcgaacg cgaccctgcc
     1681 aggccggctg ggcgcgcggc cggcggtcgc acggcggcct gagaggcggg ttggccctcg
     1741 gggaatcccg tgggatacct gggggaagat gctgcgccga accgccgcgc tccttgttgc
     1801 cgggatcggc gccagcgtcg ggctcgccgc ttgcagccac agcaagccgg cgcgcaacct
     1861 cgatctcgac ctgatccgcg tctcgcccga tgcgcgcctg cgcaccgaca ccgtcggcga
     1921 cggcaagttc gccagcacgg cgagcttcgt cctggtcgac gccgagaaca ccgccgccga
     1981 gggcgcctac gtcacgctcg gcggcgagct cacggaccct ggcggcgcca ccgtcggcga
     2041 gctcggctcg cagtcgctgt ggatcccgcc ccacgaggcc cgcacgttcg ccctggtcga
     2101 caccgagcgc ctggcccggc cgacggcagg cgccgcccgg atcaaggtcc gcggcgccct
     2161 ggtccccgac gagccgccgc gcgcccacat ctccgacctc cacagcttcg acgaccacgg
     2221 caaggtcgtg gtccaggcgt acctggtcaa cgtcgccgac cgcccgggcc agatcatggc
     2281 gatcggctcg ttccatgacg cccacggcca gccgatgacc cggccgttcc agctgctcga
     2341 ggtcggcgcc cgcgatcgcc gggtcgtcca gttcgtcggc cccgagggct cgacccgcgg
     2401 cacgatcttc gtcggcgaca cggtgtatta gggcgattcc gcggaatcgg aagccgtagg
     2461 acgttgcccc ggcccccaca ccgtgtgctc gtcaggcctc gctggggcgt ccaagcatgt
     2521 tctggacgtt ctcgtaaaga tccttctcga gcttcttgca acctcgcggc gtgtactcgt
     2581 acatgagtgc gcggcgatgt ctcagatcga atggcacgtc atccatcgat tgcgtcatga
     2641 gcaggactgg cttgttcagc gcatgtgcag ggcatccatc tgttcgacat gctccccaga
     2701 tcttggcgcc tccgtggctg gttgagccgc tggctcggcg ccggacaggg aacgcaggtc
     2761 gcggatcaga tgggcaagcg cgaggcggta ctgatcacca tcacggaaat ccgcccattg
     2821 cttggtccgg agcatcggtg ggatctcgca gtcacggtag agaatgggga ccaggcgaat
     2881 ccgatccacc ttgacttcct ctgcgagccc gtactgccat tcctgctgtg tccaggcgga
     2941 ttgaaagtag tccggcgaca tcacgatcaa catgaaccga gaggctctga tccctttctc
     3001 gatcgcaccc gcgaaggagt ctccgaccgt ggtgtcccac tggttccaga cccgcaaacc
     3061 cgctgcgacc aggtccttcg ccagacgctg cgcgaactcg cgatccttgg acgaggcgct
     3121 gaggaagacg tccaactgca gcacgcgacg cgatcagagg tcgggcaggt agtacgcggc
     3181 ggccatcgcg ccggcgccga gcgcgaccat cagcaggatc agccacacga ggttgcggct
     3241 gcgcggggca ggggcggtgt cgacgtcggc gctgggtgcc gcgcttgtct cggcgggctt
     3301 gggctgcggg cgggtcttct gggtcgactt ggcgggccgg gacttgccgg ctcgcttcgc
     3361 ggtcccggcc gctggctcga agagcgcagg aaactccgac gccgcgggag gcaccgtgag
     3421 ctcgcggcgc gatggcgccc gccgaaaccc ggggaggacc tcgagctcgc gcgacgaggc
     3481 gatgtccggt agcgcggg
//
LOCUS       DASVOR010001209         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363078,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001209 DASVOR010000000
VERSION     DASVOR010001209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363078"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF516_23025"
     CDS             <1..181
                     /locus_tag="VF516_23025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110631.1"
                     /translation="HEILSTRPSGFWTSNRPAVGGAYRWRLLGIGVVIAAITGYGMLR
                     LVRRANAERASRPRR"
     gene            181..924
                     /locus_tag="VF516_23030"
     CDS             181..924
                     /locus_tag="VF516_23030"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8110632.1"
                     /translation="MDEADPEAAETAEPIEAPPRDEIGKIIARHGPDAVCAALEPLLT
                     AERIARIDQVLAQRLGSVVTVVEDTYDPHNAAATIRTTEAIGLGELHVIEAAQRFSIA
                     GGVTRGAHRWIELYRWPSVEAVVTALRARGFRVFATLPGAPHTVDDVDVTAPLAVAFG
                     NEHAGLSPAAVAACDGALGVPMFGFTESFNLSVTVALAMSRIAARRRAAIGSPGDLDE
                     HRRRALRARWFALKIRGALGILERALGER"
     gene            1024..1299
                     /locus_tag="VF516_23035"
     CDS             1024..1299
                     /locus_tag="VF516_23035"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8110633.1"
                     /translation="MRAIGERARQLRLLRRLRQDELAERAGIGVATVHRFEKTGTASF
                     ENVLRIATALQAEAGFDKLFEPPAYSSLDEALERPAVAKRQRAPRRR"
     gene            1296..>1568
                     /locus_tag="VF516_23040"
     CDS             1296..>1568
                     /locus_tag="VF516_23040"
                     /inference="COORDINATES: protein motif:HMM:NF025041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HEX8110634.1"
                     /translation="MKLETVEQLAVFYEPSSERRHKVGRLARTQRQILFEYEPEFLGS
                     KLELSPFHLPLRPGVVVGPPELDGLMGVFDDSLPDGWGRLLIDRRAA"
BASE COUNT          215 a          544 c          581 g          228 t
ORIGIN      
        1 ccacgagatc ctgtcgaccc ggccgagcgg gttctggacc tcgaaccggc ctgcggtcgg
       61 cggcgcgtat cgctggcggc tgctcgggat cggcgtcgtg atcgccgcga tcaccggcta
      121 cggcatgctg cggctggtgc ggcgcgccaa cgccgagcgc gccagccgcc cgcgccggtg
      181 atggacgagg cggaccccga ggccgccgag acggccgagc ccatcgaggc tccgccgcgc
      241 gacgagatcg gcaagatcat cgcgcgccac ggcccggacg cggtgtgcgc cgcgctcgag
      301 ccgctgctca ccgccgagcg gatcgcgcgg atcgaccagg tgctcgccca gcggctgggc
      361 tcggtggtga cggtggtcga ggacacctac gatccgcaca acgccgcggc gacgatccgc
      421 acgaccgagg cgatcggcct gggcgagctg cacgtgatcg aggccgcaca gcggttctcg
      481 atcgccggcg gcgtgacccg cggcgcgcac cgctggatcg agctctaccg ctggccctcg
      541 gtcgaggcgg tggtgacggc gctgcgcgcg cggggattcc gggtgttcgc gaccctgccg
      601 ggcgcgccgc acacggtcga cgacgtcgac gtcacggccc cgctcgcggt cgcgttcggc
      661 aacgagcacg ccgggctgtc cccggcagcg gtcgccgcgt gcgacggcgc gctcggcgtg
      721 ccgatgttcg gcttcaccga gagcttcaac ctcagcgtca ccgtcgcgct ggcgatgagc
      781 cggatcgcgg cgcgccggcg ggccgcgatc ggcagcccgg gcgatctcga cgagcatcgc
      841 cgccgcgcgc tgcgcgcccg ctggttcgcg ctcaagatcc gtggcgcgct cggcatcctg
      901 gaacgcgcgc tcggcgagcg ctagcgggcg cgatttatag catgtcgact ttgattgatt
      961 agggcagaaa attgagctaa gttatctaaa gtgattggtt acaggagctc ctttcaggag
     1021 accttgcgcg cgatcggcga gcgcgctcgc cagctccgcc tgttgcgcag gctgcgccag
     1081 gacgagctcg ccgagcgcgc ggggatcggc gtggcgaccg tgcatcgctt cgagaagacc
     1141 gggaccgcct cgttcgagaa cgtgctccgg atcgccaccg cgctgcaggc cgaggccggc
     1201 ttcgacaagc tgttcgagcc gccggcgtac agctcgctcg acgaggcgct ggagcgtcca
     1261 gcggtcgcga agcggcagcg cgcaccgagg cgaaggtgaa gctcgagacc gtcgagcagc
     1321 tcgccgtgtt ctacgagccg agctccgagc ggcggcacaa ggtcggccgg ctggctcgca
     1381 cgcagcgcca gatcctgttc gagtacgagc ccgagttcct cggctccaag ctcgagctct
     1441 cgccgttcca cctgccgctg cgccctgggg tggtcgtggg gccgccggag ctcgatggct
     1501 tgatgggcgt gttcgacgac agcttgcccg atggctgggg gcggttgctg atcgaccggc
     1561 gggcggcc
//
LOCUS       DASVOR010001210         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_187750,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001210 DASVOR010000000
VERSION     DASVOR010001210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187750"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3468
                     /gene="rpoB"
                     /locus_tag="VF516_23045"
     CDS             <1..3468
                     /gene="rpoB"
                     /locus_tag="VF516_23045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEX8110635.1"
                     /translation="QVNYELLIGQRATRDIRSPKTKDTLVKKNRKFTKQAIRKLQDEG
                     VNVLPIDLDELVGKVSAKDVIDDSTGEVLLQCNEELTEAKLDELRERGVERAEVLFID
                     NLNVGPYLRNTLLTDKLQGPDEAVMEIYRRLRPGDQPALDSAQTLFQNLFFNPERYDL
                     SRVGRLKLNYKFKIDESIETQILTKRDILETVRYLIELRNGRGVIDDIDHLGNRRVRA
                     VGELMENQYRIGLVRMERAIKERMSMSQEIETLMPHDLINAKPVSAVVKEYFGSSQLS
                     QFMDQTNPLSEVTHKRRLSALGPGGLTRERAGFEVRDVHPTHYGRICPIETPEGPNIG
                     LIASLSTFARVNEYGFIETPYRAVEQGKISEEVQFYSALQEEGQIIAQANADVKGGKL
                     AGDYTSCRKGSDVIMTKPEDVTLMDVSPNQLVSVAASLIPFLEHDDANRALMGSNMQR
                     QAVPLIRTTSPLIGTGMEGIVARDSGVTVVAKRDGVVDWVDANRVVVRPSKVDPKDPN
                     SVRPDIYTLVKFQRSNQNTCMNQKPIVQPGDRVKAGDVIADGPATETGELALGQNVVV
                     AFMPWGGYNFEDSILINEKLVKNDVFTSIHIEEFECVARDTKLGKEEITRDIPNVGEE
                     ALKDLDESGIVRIGAEVKAGDILVGKITPKGETQLSPEEKLLRAIFGERAGDVRDTSL
                     RVPPGVVGVVIGARVFARKGTEKDERAQAIEDAEKELLMLNKRDEVKIISEAYYNKMR
                     ELLVGKTTASRLVDDKGKVLLPKGEKITLEMLNEVPSRYWHEIQLDAGDGKIEEQLEQ
                     LAAKREEDVFNIEQQYSEKISKLTKGDELPPGVIKLVKVYLAIKRKLSVGDKMAGRHG
                     NKGVVSRLLPEEDMPYLEDGTPVDIVLNPLGVPSRMNIGQILEVHLGWAAKSLGNQIQ
                     AYLDTNWNADVLKDKLRKLFPDHTALIDRLAPEDTGRFARTLTSGIHLATPVFDGATE
                     HEIKSALKMAGLPAGGQARLIDGKTGEPFDNMVTVGVMYMLKLHHLVDDKIHARSIGP
                     YSLVTQQPLGGKAQFGGQRLGEMEVWAMEAYGAAYALQEFLTVKSDDVLGRTRMYESI
                     VKGEHVLEAGLPESFNVLLKELQALCLDVELIEDPTVPRRQTEQAPGIPAGLAALARE
                     VADKMGSAS"
     gene            3510..>5879
                     /gene="rpoC"
                     /locus_tag="VF516_23050"
     CDS             3510..>5879
                     /gene="rpoC"
                     /locus_tag="VF516_23050"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX8110636.1"
                     /translation="MKDIFNFFEKPKDPRSFSAIRIMLASPEKIREWSHGEVKKPETI
                     NYRTFKPERDGLFCAKIFGPVKDYECNCGKYKRMKHRGVVCEKCGVEVIQAKVRRERM
                     GHISLATPVAHIWFLKSLPSRIGNLLDIPLKDLEKVLYCEAYIVTNAKQSGLSPCELL
                     TEERYSKLLDELGFDAFDAGMGAEAILQLLKDINIVEMAETLRQEMRDITSEAKRKKI
                     AKRLKVVEAFRESGNKPEWMMLTIIPVIPPDLRPLVPLDGGRFATSDLNDLYRRVINR
                     NNRLRRLQELNAPEIIIRNEKRMLQEAVDALFDNGRRGKTITGPNKRPLKSLSDMLKG
                     KQGRFRQNLLGKRVDYSGRSVIVIGPELKLHQCGLPKKMALELFTPFIYNKLEERHLV
                     TTIKSAKKLVEKERPEVWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLTEGKAIQL
                     HPLVCAAFNADFDGDQMAVHVPLSIEAQMEARVLMMSTNNILSPANGKPIIVPSQDIV
                     LGLYHLTRERPYARGEFREAKNGQAARGVYGSVSEVRMAYDHGEVDLQAAIKLRMRKP
                     DGDGTELVSTTVGRALLRDICPRQIPFEAINKVMGKKQLAELIDLVYREAGQKATVLL
                     ADALRTTGYTYATRAGISICLDDMVIPPSKEVLLDEAKKEVAEIEEQYTEGLITDGER
                     YNKVVDIWAQVAEAIAKDMMKEISTEEFKDPKTGATKKAPSFNSIFIMADSGARGSQQ
                     QMRQLAGMRGLMAKPSGEIIETPITTNFREGLSVLQYFISTHGARKGLAD"
BASE COUNT         1163 a         1986 c         1927 g          803 t
ORIGIN      
        1 caggtcaact acgagctgct gatcggccag cgcgcgacgc gcgacatccg cagccccaag
       61 accaaggaca cgctggtcaa gaagaaccgc aagttcacca agcaggcgat ccgcaagctg
      121 caggacgagg gggtcaacgt cctgccgatc gatctcgacg agctggtcgg caaggtctcg
      181 gccaaggacg tgatcgacga ctcgaccggc gaggtgctgc tccagtgcaa cgaggagctg
      241 accgaggcca agctcgacga gctccgcgag cgcggcgtcg agcgcgccga ggtcctgttc
      301 atcgacaacc tcaacgtcgg cccgtacctg cgcaacacgc tgctcaccga caagctgcag
      361 ggccccgacg aggcggtcat ggagatctac cgccggctgc gcccgggcga ccagcccgcg
      421 ctcgactcgg cgcagaccct gttccagaac ctgttcttca accccgagcg ctatgacctg
      481 tcgcgggtcg gccggctcaa gctcaactac aagttcaaga tcgacgagtc gatcgagacc
      541 cagatcctga ccaagcgcga catcctcgag accgtccgct acctgatcga gctgcgcaac
      601 ggccgcggcg tgatcgacga catcgatcac ctcggcaacc gccgggtgcg cgcggtcggc
      661 gagctgatgg agaaccagta ccggatcggc ctggtccgca tggagcgcgc gatcaaggag
      721 cgcatgagca tgtctcagga gatcgagacg ctcatgccgc acgacctgat caacgccaag
      781 ccggtcagcg cggtggtcaa ggagtacttc ggcagctcgc agctgtcgca gttcatggat
      841 cagaccaacc cgctgtccga ggtcacccac aagcgccggc tgtcggcgct cgggcccggc
      901 ggtctgaccc gcgagcgcgc gggcttcgag gtccgcgacg tccacccgac gcactacggc
      961 cggatctgcc cgatcgagac cccggaaggt ccgaacatcg gcctgatcgc gtcgctgtcg
     1021 acgttcgcgc gggtcaacga gtacggcttc atcgagaccc cgtaccgcgc ggtcgagcag
     1081 ggcaagatct ccgaggaggt ccagttctac tcggcgctcc aggaggaggg ccagatcatc
     1141 gcccaggcca acgccgacgt gaagggcggc aagctcgcgg gcgactacac gtcgtgccgc
     1201 aagggctcgg acgtgatcat gaccaagccc gaggacgtca cgctgatgga cgtgtcgccc
     1261 aaccagctgg tctcggtcgc ggcgtcgctg atcccgttcc tcgagcacga cgacgccaac
     1321 cgcgcgctga tggggtcgaa catgcagcgc caggcggtgc cgctgatccg caccacctcg
     1381 ccattgatcg gaaccggcat ggagggcatc gtcgcgcgcg actccggcgt caccgtggtc
     1441 gccaagcgcg acggcgtggt cgactgggtc gacgccaacc gcgtcgtcgt ccggccgagc
     1501 aaggtcgacc ccaaggaccc caactcggtc cgccccgaca tctacacgct ggtcaagttc
     1561 cagcgcagca accagaacac gtgcatgaac cagaagccga tcgtccagcc cggcgatcgg
     1621 gtcaaggccg gcgacgtgat cgccgacggt ccggcgaccg agaccggcga gctcgcgctc
     1681 ggccagaacg tggtcgtcgc gttcatgccg tggggtgggt acaacttcga ggactcgatc
     1741 ctgatcaacg agaagctggt caagaacgac gtcttcacct cgatccacat cgaggagttc
     1801 gagtgcgtcg cgcgcgacac caagctcggc aaggaggaga tcacgcgcga catcccgaac
     1861 gtcggcgagg aggcgctcaa ggacctcgac gagtcgggca tcgtccggat cggcgccgag
     1921 gtcaaggccg gcgacatcct ggtcggcaag atcacgccca agggcgagac ccagctgtcg
     1981 cccgaggaga agctcttgcg cgcgatcttc ggcgagcgcg cgggcgacgt ccgcgacacc
     2041 tcgctgcgcg tgccgcccgg cgtggtcggc gtcgtgatcg gcgcccgcgt gttcgcgcgc
     2101 aagggcaccg agaaggacga gcgcgcccag gcgatcgagg acgccgaaaa agagctgctg
     2161 atgctcaaca agcgcgacga ggtgaagatc atctcggagg cgtactacaa caagatgcgc
     2221 gagctcctgg tcggcaagac cacggcgtcg cgcctggtcg acgacaaggg caaggtcctc
     2281 ctgcccaagg gcgagaagat cacgctcgag atgctcaacg aggtcccgag ccgctactgg
     2341 cacgagatcc agctcgacgc cggcgacggc aagatcgagg agcagctcga gcagctcgcc
     2401 gccaagcgcg aggaggacgt gttcaacatc gagcagcagt actcggagaa gatctccaag
     2461 ctgaccaagg gcgacgagct gccgcccggc gtgatcaagc tggtcaaggt ctacctcgcg
     2521 atcaagcgca agctgtcggt cggcgacaag atggccggcc gccacggcaa caagggcgtg
     2581 gtgtcgcggc tgctccccga ggaggacatg ccgtacctcg aggacggcac cccggtcgac
     2641 atcgtgctca acccgctcgg cgtgccgagc cgcatgaaca tcggccagat cctcgaggtc
     2701 cacctcggct gggcggccaa gtcgctcggc aaccagatcc aggcctacct cgacaccaac
     2761 tggaacgccg acgtgctcaa ggacaagctg cgcaagctgt tccccgacca cacggcgctg
     2821 atcgaccggc tcgcccccga ggacaccggc cggtttgccc ggacgctcac gagcggcatc
     2881 cacctggcga ccccggtgtt cgacggcgcg accgagcacg agatcaagtc ggcgctcaag
     2941 atggccgggc tgccggccgg cggacaggcc cggctgatcg acggcaagac cggcgagccg
     3001 ttcgacaaca tggtcaccgt cggcgtgatg tacatgctca agctccacca cctggtcgac
     3061 gacaagatcc acgcgcggag catcggcccg tactcgctgg tgacccagca gcctctgggt
     3121 ggcaaggcgc agttcggcgg ccagcggctc ggcgagatgg aggtctgggc gatggaggcc
     3181 tacggcgcgg cctacgcgct gcaggagttc ctgaccgtca agtccgacga cgtgctcggc
     3241 cggacccgga tgtacgagtc gatcgtcaag ggcgagcacg tgctcgaggc cggtctgccc
     3301 gagtcgttca acgtgctgct caaggagctc caggcgctgt gcctggacgt cgagctgatc
     3361 gaggacccga ccgtgccccg ccgccagacc gagcaggccc ccggcatccc cgccgggctg
     3421 gccgcgctcg cccgcgaggt cgccgacaag atgggcagcg cgagctgatc ccggaacgaa
     3481 cgaaccccac gagtcaacag gagaactaag tgaaggacat cttcaacttc ttcgagaagc
     3541 cgaaggatcc acgttcgttc agcgcgatcc ggatcatgct cgcctccccg gagaagatcc
     3601 gcgagtggtc gcacggcgag gtcaagaagc ccgagacgat caactaccgg acgttcaagc
     3661 cggagcgcga cggcctgttc tgcgccaaga tcttcgggcc ggtcaaggac tacgagtgca
     3721 actgcggcaa gtacaagcgc atgaagcacc gcggggtggt gtgcgagaag tgcggcgtcg
     3781 aggtcatcca ggccaaggtc cgccgcgagc gcatgggcca catctcgctg gctacccccg
     3841 tcgcgcacat ctggttcctc aagagcctgc cgtcgcggat cggcaacctg ctcgacatcc
     3901 cgctcaagga cctcgagaag gtcctgtact gcgaggcgta catcgtcacc aacgccaagc
     3961 agtcggggct gtcgccgtgc gagctgctca ccgaggagcg ctactccaag ctgctcgacg
     4021 agctcgggtt cgacgcgttc gacgccggga tgggcgccga ggcgatcctc cagctgctca
     4081 aggacatcaa catcgtcgag atggcagaga ccctgcgcca ggagatgcgc gacatcacct
     4141 ccgaggccaa gcgcaagaag atcgccaagc ggctcaaggt cgtcgaggcg ttccgcgaga
     4201 gcggcaacaa gcccgagtgg atgatgctga ccatcatccc ggtgatcccg cccgacctgc
     4261 gcccgctggt gccgctcgac ggcggccggt tcgcgaccag cgacctcaac gacctgtacc
     4321 gccgcgtgat caaccggaac aaccggctcc gccggctcca ggagctcaac gcgcccgaga
     4381 tcatcatccg caacgagaag cggatgctgc aggaggcggt cgacgcgctg ttcgacaacg
     4441 gccggcgcgg caagaccatc accgggccca acaagcggcc gctcaagtcg ctgtcggaca
     4501 tgctcaaggg caagcagggc cggttccgcc agaacctgct cggcaagcgg gtcgactact
     4561 cgggccgctc ggtgatcgtg atcggccccg agctcaagct gcaccagtgc ggcctgccca
     4621 agaagatggc gctcgagctg ttcacgccgt tcatctacaa caagctcgag gagcgccacc
     4681 tggtcaccac gatcaagagc gccaagaagc tggtcgagaa ggagcgcccc gaggtgtggg
     4741 acatcctcga ggaggtgatc aaggagcacc cggtgctgct caaccgcgcg ccgaccctgc
     4801 accgcctcgg catccaggcg ttcgagccgg tgctgaccga gggcaaggcg atccagctcc
     4861 acccgctggt gtgcgcggcg ttcaacgccg acttcgacgg cgaccagatg gcggtccacg
     4921 tgccgctgtc gatcgaggcc cagatggagg cccgcgtgct catgatgagc acgaacaaca
     4981 tcctgtcgcc ggccaacggc aagccgatca tcgtgccatc gcaggacatc gtgctcggcc
     5041 tgtatcacct gacccgcgag cggccgtatg ccaggggcga gttccgcgag gccaagaacg
     5101 gccaggcggc gcgcggcgtg tacggctcgg tctccgaggt ccggatggcg tacgaccacg
     5161 gcgaggtcga cctccaggcc gcgatcaagc tgcgcatgcg caagcccgac ggcgacggca
     5221 ccgagctggt gtcgaccacg gtcggccgcg cgctgctgcg cgacatctgc ccgcgccaga
     5281 tcccgttcga ggcgatcaac aaggtcatgg gcaagaagca gctcgccgag ctgatcgacc
     5341 tcgtgtatcg cgaggccggc cagaaggcca cggtgctgct cgccgacgcg ctgcgcacca
     5401 ccgggtacac ctacgcgacc cgcgcgggca tctcgatctg cctcgacgac atggtgatcc
     5461 cgccgtcgaa ggaggtcctg ctcgacgagg ccaagaagga ggtcgccgag atcgaggagc
     5521 agtacaccga gggcctgatc accgacggcg agcgctacaa caaggtcgtc gacatctggg
     5581 cccaggtcgc cgaggcgatc gccaaggaca tgatgaagga gatctcgacc gaggagttca
     5641 aggaccccaa gaccggggcg accaagaagg cgccgtcgtt caactcgatc ttcatcatgg
     5701 ccgactccgg cgcgcgcggc tcgcagcagc agatgcggca gctcgccggc atgcgcggcc
     5761 tgatggccaa gccgtcgggc gagatcatcg agaccccgat cacgaccaac ttccgtgagg
     5821 ggctgtcggt gctgcagtac ttcatctcga cccacggcgc gcgcaagggc ctggccgac
//
LOCUS       DASVOR010001211         1979 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_246172,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001211 DASVOR010000000
VERSION     DASVOR010001211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246172"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1362
                     /locus_tag="VF516_23055"
     CDS             <1..1362
                     /locus_tag="VF516_23055"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8110637.1"
                     /translation="VVRGNFKTRDGIILSELGLRRGARLTQDALAEGARRLRNTGLFD
                     AVSVTLPDLESTRAGEVNAVVEVVERYDLLLQADAEVGYSSYNGAFLKLIPSFKNLFG
                     IGMSFDVAGTIGLDLAEYLRNGDTKLRQLSAEATLRVPQWLSRRFSPVEFQTELTAFH
                     RLQDTPRFGQVTTDGVTVALSRTWDRKRTATRDARAITTGLHYDFRIRERPIDVLRPL
                     GADDDQTQVPISTQTGSVGVTFEWEQRVDRQHALSPLAPEGGFRFDAQLSMASTRLGG
                     QDDFVKVSAGGSRYLPLGQSLVLRADLRFDEGFPLSGAVLLPEVERFFAGGDASVRGY
                     NDDRLATEIVQVNVPPVGNVQQIRILPAGGNIRVMSSLDAQIRIWRLLATGVFVDAGM
                     IANEWRAVTTNDIRPSIGMALLRIVTPFGVGAAEYAIPLRPELGDDPRGRWHISFAAR
                     AQF"
     gene            1473..>1979
                     /locus_tag="VF516_23060"
     CDS             1473..>1979
                     /locus_tag="VF516_23060"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEX8110638.1"
                     /translation="MNTHAVQIDRSKSLAEQPGTGHNRWHEGIPPILRIQPGDQVILE
                     TRDALDGQITPSSTVDDVGRANLGVAHALTGPVYVEGAEPGDLLEVKIQAIHPERFGF
                     TVQVPGFSFLRDLFPEPHIVRWDIHGGFAESRDLPGVRIPGAPFMGVMGVAPSRELRE
                     SLRAREAAL"
BASE COUNT          280 a          753 c          673 g          273 t
ORIGIN      
        1 gtggtccgcg gcaacttcaa gacccgcgac gggatcatcc tcagcgagct cggcctgcgg
       61 cgcggcgcgc ggctgaccca ggacgcgctc gccgagggcg cgcggcggct gcgcaacacc
      121 gggctgttcg atgcggtcag cgtgacgctg ccggacctgg agtcgacccg ggccggcgag
      181 gtcaacgcgg tggtcgaggt cgtcgagcgc tacgacctcc tgctccaggc cgacgccgag
      241 gtcggctact cgagctacaa cggcgcgttc ctcaagctga tcccgtcgtt caagaacctg
      301 ttcgggatcg gcatgtcgtt cgacgtcgcc ggcacgatcg gtctcgacct cgccgagtac
      361 ctgcgcaacg gcgacaccaa gctgcgccag ctcagcgccg aggccacgct gcgcgtcccg
      421 cagtggctgt cgcgccggtt ctcgccggtc gagttccaga ccgagctgac cgcgttccac
      481 cgcctccagg acacgccgcg gttcggccag gtcacgaccg acggcgtcac cgtcgcgctg
      541 tcgcggacct gggaccgcaa gcggaccgcc acgcgcgacg cccgcgcgat caccaccggc
      601 ctgcactacg acttccgcat ccgcgagcgc ccgatcgacg tgctgcgccc gctcggcgcc
      661 gacgacgatc agacccaggt cccgatctcg acccagaccg gctcggtcgg cgtgaccttc
      721 gagtgggagc agcgcgtcga tcgccagcac gcgctgtcgc cgctcgcccc cgagggcggg
      781 ttccggttcg acgcccagct gtcgatggcc agcaccaggc tcggcggcca ggacgacttc
      841 gtcaaggtct cggccggcgg cagccgctac ctaccgctcg gccagagcct ggtgctgcgc
      901 gccgacctcc ggttcgacga ggggttcccg ctgagcggcg cggtcctgct ccccgaggtc
      961 gagcggttct tcgccggcgg cgacgcgagc gtgcgcggct acaacgacga ccggctcgcc
     1021 accgagatcg tccaggtcaa cgtcccgccg gtcggcaacg tccagcagat ccggatcctg
     1081 cccgccggcg gcaacatccg cgtgatgtcc agcctcgacg cccagatccg gatctggcgg
     1141 ctgctcgcga ccggcgtgtt cgtcgacgcc ggcatgatcg ccaacgagtg gcgcgcggtc
     1201 accaccaacg acatccggcc atcgatcggc atggcgctgc tccgcatcgt caccccgttc
     1261 ggggtcggcg ccgccgagta cgcgatcccg ctgcgccccg agctcggcga cgacccgcgc
     1321 ggccgctggc acatcagctt cgccgcccgc gcccagttct agcggccgga cttcacgact
     1381 ttctggtgaa cgatgcgcgt acgggagtgc cgccgcgcgc tttgcggccg ggccggccgg
     1441 cgcgaacatc cgcacacacc cagggggtca cgatgaacac gcacgccgtc cagatcgatc
     1501 gctccaagtc gctcgccgag cagccgggca ccggacacaa ccgctggcac gaggggatcc
     1561 cgccgatcct gcggatccag cccggcgatc aggtcatcct cgagacccgc gacgcgctcg
     1621 acgggcagat cacgccgagc tcgaccgtcg acgacgtcgg ccgcgccaac ctcggggtgg
     1681 cccacgcgct gaccgggccg gtgtacgtcg agggcgccga gcccggcgac ctgctcgagg
     1741 tcaagatcca ggcgatccac cccgagcggt tcggcttcac cgtgcaggtg ccgggcttca
     1801 gcttcctgcg cgacctgttc cccgagcccc acatcgtgcg ctgggacatc cacggcggct
     1861 tcgccgagtc gcgcgacctg cccggggtcc ggatcccggg cgcgccgttc atgggcgtga
     1921 tgggggtcgc gccgtcgcgc gagctgcgcg agagcctgcg ggcgcgcgag gcggcgctg
//
LOCUS       DASVOR010001212         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_333609,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001212 DASVOR010000000
VERSION     DASVOR010001212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333609"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF516_23065"
     CDS             complement(<1..294)
                     /locus_tag="VF516_23065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110639.1"
                     /translation="MLAACSPGADTAGSDPDRGHLIALNSQVIDIRLAAPAVASTPGD
                     DRVLVKFPGPVTAAQLQALAASAQIDAYLPYDTFLVRPLHGAAAMATAAAAPAL"
     gene            complement(639..>2793)
                     /locus_tag="VF516_23070"
     CDS             complement(639..>2793)
                     /locus_tag="VF516_23070"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8110640.1"
                     /translation="RPAKAAPPAVSVEGVRPRIVQAAKPQNAAAASLRQSAAVGESYL
                     NDLLTGGLLDVAGVRVPDADFDLRPDRRWGRSTRRAFVFLFVVLVLGIGGGGTWYWWS
                     EKQKAETVARLQHESQVAIQLGDHDGFETCTKKLADALKLDKASLLTYAYFAECAGLD
                     TLLYGTDPDNADNALKVAREIKPEQPGAREVLIGRAAVELAQLGLKVSAPNSSQTAIA
                     QVAKTTLAEQRKALDAYAKQHDKDRWVMWLQGRALLAGGERKAARAMLKAASDGDDGL
                     VVAMIDSADLLVDDGQLDDALAIYDRAAARVKEHPPAKDQQPKPHPLIVVSRVIGRVE
                     AAQVTDETLGELSVNLTGTLPGRLQAYRDLALALAATVTQDNVKAKETLQKAAGGSRP
                     PNEPRFWARVAWAHVMRGDVAGTIGARSRIVWFGQSKAEDDPVVQLVDDGLLLAAGLP
                     DKALALAERLQGVRPQILRAYAELDLGKPRDALKEADAVLKKSPDSMEAKILREQARM
                     ESTEGKERADATEALEKLARQAKNKTGLHALGVAHFVNGNLKDAQARLEQAVADLSDE
                     APNPLAYRSYTTLAEILLTNGDLPGARKAAEAANKLNPGYFPANGVLAKVALRQGEAD
                     HAYDLLQPMVKEMGDTMPAATQLVYAEAMATKKDADKARAVELLKKIKDQVPQPELSR
                     AAAAIDPKLPKELGIPEPTAPDAAKPAEPKRPKRR"
BASE COUNT          337 a          978 c         1025 g          453 t
ORIGIN      
        1 gagcgccggg gcggcggccg ccgtggccat cgccgccgcg ccgtgcagcg gccggaccag
       61 gaacgtgtcg tacggcaggt aggcgtcgat ctgcgcgctc gccgccagcg cctggagctg
      121 cgccgcggtc accgggccgg ggaacttcac cagcacgcgg tcgtcgcccg gcgtcgacgc
      181 caccgcgggc gcggcgagcc ggatgtcgat gacctgcgag ttgagcgcga tcagatgacc
      241 gcgatccgga tcggatccgg cggtgtcggc gcccggactg caggcggcca gcaagctgag
      301 gatgacaacg agccgcgcga cgcgacgcgc ggaggggtga atggcccggc tttgccgggc
      361 cggagggggc gggaacgccc cctggggatc gatgagccaa atcgactggc gacggactgt
      421 catggtgtgc ctccctgggc ggggacacca gcacgcgcgc gtgatcctcg acaaggcttg
      481 cgcgaggcaa tctgcctcgg gtcacaaata tcgtatctcg gccaacatgc cagtcgagat
      541 gcgatgtatg ctactgacat ccatcgcctc gattggtgct tcgacccttc gaccggccag
      601 ttggtcaccg cgcgggagcg tgccgggaac gcgatcactc agcggcgctt ggggcgcttg
      661 ggctcggccg gcttggcggc gtcgggagcg gtcggctcgg ggatgccgag ctccttgggc
      721 agcttgggat cgatcgccgc ggcggcgcgc gacagctcgg gctgcggcac ctggtccttg
      781 atcttcttca gcaactcgac ggccctggcc ttgtccgcgt ccttcttggt cgccatcgcc
      841 tcggcgtaga ccagctgcgt cgccgccggc atggtgtccc ccatctcctt caccatcggc
      901 tgcagcagat cgtacgcgtg atcggcctcg ccctggcgca gggcgacctt ggccagcacg
      961 ccgttggccg ggaagtagcc cgggttgagc ttgttcgccg cctcggccgc cttgcgggcg
     1021 ccgggaagat cgccgttggt gagcaggatc tcggcgagcg tggtgtacga gcggtacgcg
     1081 agcgggttgg gcgcctcgtc gctgagatcg gcgaccgctt gctcgagccg ggcctgcgcg
     1141 tccttgaggt tgccgttgac gaagtgcgcg acgccgagcg cgtggaggcc ggtcttgttc
     1201 ttggcctggc gcgcgagctt ctccagcgcc tcggtggcgt cggcgcgctc cttgccctcg
     1261 gtgctctcca tccgcgcctg ctcgcgcagg atcttggcct ccatgctgtc cggcgacttc
     1321 ttgagcacgg cgtcggcttc cttgagcgcg tccctgggct tgccgagatc gagctcggcg
     1381 taggcgcgca ggatctgcgg ccgcacgccc tgcagccgct cggcgagcgc gagcgccttg
     1441 tcgggcagcc cggccgccag caacagcccg tcgtcgacca gctggaccac cggatcgtcc
     1501 tcggccttgc tctggccgaa ccacacgatc cgggagcgtg ccccgatggt gccggccacg
     1561 tcgccgcgca tgacgtgggc ccacgcgacc cgcgcccaga accgcggctc gttgggcggg
     1621 cggctgccgc cggccgcctt ctgcagcgtc tccttggcct tgacgttgtc ctgggtcacg
     1681 gtggcggcga gcgccagcgc gagatcgcgg tacgcctgga gccgccccgg tagcgtgccg
     1741 gtcaggttga cgctgagctc gccgagcgtc tcgtcggtca cctgcgcggc ctcgacccgg
     1801 ccgatcacgc ggctgacgac gatcagcgga tgcggcttgg gctgctggtc cttggccggg
     1861 ggatgctcct tgacccgggc cgccgcccgg tcgtagatcg ccagcgcgtc gtcgagctgg
     1921 ccgtcgtcga ccagcaggtc ggcgctgtcg atcatcgcga cgaccaggcc gtcgtcgccg
     1981 tcggacgcgg ccttgagcat cgcgcgcgcg gccttgcgct cgccgccggc gagcagggcg
     2041 cggccctgca gccacatgac ccagcggtcc ttgtcgtgct gcttggcgta ggcgtcgagc
     2101 gccttgcgct gctcggcgag cgtggtcttg gcgacctggg cgatcgcggt ctgcgagctg
     2161 ttgggcgcgc tgaccttgag gccgagctgc gcgagctcga ccgccgcgcg gccgatcagc
     2221 acctcgcgcg cacccggctg ctcgggcttg atctcgcgcg ccaccttgag cgcgttgtcg
     2281 gcgttgtcgg ggtcggtgcc gtacagcagg gtgtccaggc cggcgcactc cgcgaagtac
     2341 gcgtaggtga gcaggctggc cttgtcgagc ttgagcgcgt cggcgagctt cttggtgcag
     2401 gtctcgaagc cgtcgtggtc gccgagctgg atcgcgacct gcgactcgtg ctgcagccgc
     2461 gccacggtct cggccttctg cttctcgctc caccagtacc aggtgccgcc gccgccgatc
     2521 ccgagcacca gcacgacgaa caggaacacg aacgcgcggc gcgtcgaccg cccccaccgc
     2581 cggtcgggcc ggagatcgaa gtcggcgtcg gggacgcgca cgccggcgac atcgagcagc
     2641 ccgccggtca gcaggtcgtt gaggtagctc tcgccgaccg cggccgactg gcgcagcgac
     2701 gcggcggcgg cgttctgcgg cttggcggcc tgcacgatgc gcggccggac gccctcgaca
     2761 ctgaccgccg gcggcgcggc cttggccggg cgc
//
LOCUS       DASVOR010001213         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_567947,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001213 DASVOR010000000
VERSION     DASVOR010001213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_567947"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1728
                     /locus_tag="VF516_23075"
     CDS             <1..>1728
                     /locus_tag="VF516_23075"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8110641.1"
                     /translation="LELARAGRLTAPYGAPFELVHEERVYRLRRYARDAVATAPAAPL
                     LLVPPLMVAAEVYDISPDVSAVAYLGRAGVDVWLVDFGAPEREEGGMSRTLDDHVRAV
                     SQAVERVRAATGRDVHLAGYSQGGMFCYQAAAFRRSANIASILAFGSPVDLHRTLQVG
                     EDATERLLGGLRAALAWPLARIEGLPGFLTSTGFKLLSARKEVRQLVDFVKNLHDREA
                     LEKREAKRLFLGGEGFVAWPGPAFRKFVDELIVGNRMASGGFVIDGHTVTLADITCPI
                     LYAVGSRDEMGRPAAVRGIQRACPRVRVMYELPVTAGHFGLVVGSTALTVTWPSVVDW
                     MRWREGLGGPPAGASDVRAYPVRDPVAAADDADDHDTDDADHDDPEDDDDAGGGPGRL
                     DLELARALVDKTARVIADRLSELSVDLGDYLDNARYQLPRLARLRHLQDDSRISFSKT
                     LADQAREIGDRTFFLFRGRAFSYADANRRVDAVVRGLIASGIRPGDRVGVMMKSRPSH
                     LSAVTAINRIGAVAVLLSPVTPDDAVPRALALGEADVLVVDPDSAARARAAITGRIPL
                     AIRPRTTAST"
BASE COUNT          208 a          673 c          617 g          230 t
ORIGIN      
        1 ctcgagctcg cgcgcgccgg tcggctgacc gcgccgtacg gtgcgccgtt cgagctcgtc
       61 cacgaggagc gcgtgtaccg gctgcggcgc tacgcgcgcg acgcggtcgc gacggcgccg
      121 gccgccccgc tgctcctggt cccgccgctg atggtcgccg ccgaggtcta cgacatctcg
      181 cccgacgtca gcgccgtggc gtacctcggg cgcgccggcg tcgacgtctg gctggtcgac
      241 ttcggcgcgc cggagcgcga ggagggcggc atgagccgca cgctcgacga ccacgtccgc
      301 gcggtgtccc aggcggtcga gcgcgtgcgc gccgccaccg gccgcgacgt ccacctcgcc
      361 ggctactcgc agggcgggat gttctgctac caggccgcgg cgttccggcg ctcggcgaac
      421 atcgcctcga tcctcgcgtt cggcagcccg gtcgacctcc accgcacgct gcaggtcggc
      481 gaggacgcca ccgagcgcct gctcgggggc ctgcgcgccg cgctggcgtg gccgctggcg
      541 cggatcgagg gcctgcccgg cttcttgacc tcgacggggt tcaagctcct gtcggcgcgc
      601 aaggaggtgc gccagctggt cgacttcgtc aagaacctgc acgaccgcga ggcgctggag
      661 aagcgcgagg ccaagcggct gttcctcggc ggcgagggct tcgtcgcctg gcccgggccg
      721 gcgttccgca agttcgtcga cgagctgatc gtcggcaacc gcatggcgtc gggcggcttc
      781 gtgatcgacg gccacaccgt gacgctcgcc gacatcacct gcccgatcct ctacgccgtc
      841 ggcagccgcg acgagatggg ccgcccggcc gcggtccgcg gcatccagcg cgcctgcccg
      901 cgggtccgcg tgatgtacga gctgccggtc acggcaggcc acttcggcct ggtcgtcggc
      961 tccaccgcgc tcaccgtcac ctggccgagc gtcgtcgact ggatgcggtg gcgcgagggc
     1021 ctcggcgggc cgcccgccgg cgcgagcgac gtccgcgcct accccgtgcg cgatccggtc
     1081 gcggccgcgg acgacgcgga cgaccatgac acagacgacg cagaccacga tgacccggag
     1141 gacgacgacg atgcgggcgg cgggccgggc cgcctcgacc tcgagctcgc ccgcgcgctg
     1201 gtcgacaaga ccgcgcgggt catcgccgac cggctgtccg agctgtcggt cgacctcggc
     1261 gactacctcg acaacgcgcg ctaccagctg ccgcggctcg cccggctccg ccacctgcag
     1321 gacgactcgc ggatctcgtt cagcaagacc ctggccgacc aggcgcgcga gatcggcgac
     1381 cgcaccttct tcctgttccg cggccgcgcg ttcagctacg ccgacgccaa ccgccgggtc
     1441 gatgccgtcg ttcgcggcct gatcgccagc ggcatccgcc ccggcgaccg ggtcggcgtg
     1501 atgatgaagt cgcggccgag ccatctctcg gcggtcaccg cgatcaaccg gatcggcgcg
     1561 gtcgccgtgc tgctgtcgcc ggtgacgccc gacgacgccg tgccgcgcgc cctggcgctc
     1621 ggcgaggccg acgtcctcgt cgtcgatccc gacagcgccg cccgcgcccg cgccgcgatc
     1681 accggccgga tcccgctggc gatcaggccg cggacgaccg cgtcgacc
//
LOCUS       DASVOR010001214         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_216937,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001214 DASVOR010000000
VERSION     DASVOR010001214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216937"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..511)
                     /locus_tag="VF516_23080"
     CDS             complement(<1..511)
                     /locus_tag="VF516_23080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110642.1"
                     /translation="MEVFFLMMGLWLVVAIALGMRRPRGFGGAALRARLDAVYGEPHE
                     LARVSPAAFPEADLEFYDRARAAFERRGYRWIADVEDLTLTRIYPQNRTFLRLFVDAG
                     SMIRASVYHLHPRGIVLSMLQLVQLYPRHLRVIELVSEIQGVFLVTSNTHGVDRLEPP
                     PEARIERLPL"
     gene            complement(630..2144)
                     /locus_tag="VF516_23085"
     CDS             complement(630..2144)
                     /locus_tag="VF516_23085"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8110643.1"
                     /translation="MLENQSFDRMFAMSGIPGIIAATTRDSNSHDDTAYHVQNGAPVV
                     MPTDPGHEFLDVVEQLGGEGAVYPPGGPYPAINNSGFAANYATSTTEGPASPPQDIGD
                     IMACFGTQSQLPVFYQLATQFALCDQWFSSLPGPTWPNRYFVHGASSSGLDDSPSLVQ
                     MGTWEIDGFPFPHGSIFQALDDASIQYRLYSDFNPSPGSSYGLSLFGDDVQNGSLAGA
                     IPQASSLQGVSLMDFHSLLHFASDLQGPYPYTYTFIEPHYGDIVRGSYTGGSSQHPVD
                     DMAGGQKLVQAVYEAIRNSPYWNSSLLIITFDEHGGFYDSVAPGTATAPGDHPGDGFN
                     AYGFTFEQYGVRVPTIVVSPLIAAGTVDHTVYDHASVLATLERLFGLKALTQRDAAAS
                     DVTHLFTQAEPRGDCPAKLSSPAPLLEAARRPLPAEELAEIDQQPLPASGNLPGLLSI
                     LLKTEIELSSRTPAAIAGLVAKVKGIKTRGEARAYIASVMDKVRAARAASVRTH"
     gene            2116..2382
                     /locus_tag="VF516_23090"
     CDS             2116..2382
                     /locus_tag="VF516_23090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110644.1"
                     /translation="MRSNDWFSSITTNTFVIGEYGASFVIPPVHPPESRATSKVVVST
                     DRVMVLPASPCCSWHSFQYGTIAVPLVMYCPGVDPPLTLSITDQ"
BASE COUNT          416 a          815 c          857 g          401 t
ORIGIN      
        1 ccagcggcag ccgctcgatc cgcgcctcgg gcggcggctc cagccggtcg acgccgtggg
       61 tgttgctggt caccaggaac acgccctgga tctcgctgac cagctcgatc acgcgcaggt
      121 ggcgcgggta gagctggacc agctgcagca tcgacagcac gatgccgcgc gggtgcaggt
      181 ggtacacgct ggcgcggatc atgctgccgg cgtcgacgaa cagccgcagg aacgtccggt
      241 tctgcgggta gatccgggtc agcgtcaggt cctcgacgtc ggcgatccag cgatagcccc
      301 ggcgctcgaa cgccgcgcgg gcgcggtcat agaactcgag gtcggcctcg gggaacgccg
      361 ccggcgacac ccgcgccagc tcgtggggct cgccgtacac cgcgtcgagc cgggcgcgca
      421 gcgccgcccc gccgaagccg cgcggccggc gcatgccgag cgcgatcgcg acgacgagcc
      481 acagccccat catgaggaag aacacttcca cggccgcatt gtgccatgcg ccggggacgc
      541 tggcttcgcc gatcccgcgg atgcgcgccc gcgcacccgg ccgtgggtgc agccgtcaag
      601 gacgggctcg cgaagcggtt acctccacgt caatgcgtgc ggacgctggc ggcgcgggcg
      661 gccctcacct tgtccatcac cgacgcgatg taggcccgtg cctcgccgcg cgtcttgatg
      721 cccttgacct tcgcgacgag ccctgcgatg gccgccggcg ttcgagacga cagctcgatc
      781 tccgtcttca gcaggatcga tagtaggccc ggcaggttcc cggacgccgg cagcggctgc
      841 tggtcgatct cggccagctc ctcggcgggc agcgggcgcc tggccgcttc gagcagcggc
      901 gccggggagc tcagcttggc cgggcaatcg ccgcgcggct ccgcctgggt gaacaggtgg
      961 gtcacgtcgc tggcggcggc gtctcgctgg gtgagcgcct tcaacccgaa caggcgctcc
     1021 agcgtggcga gcaccgaggc gtggtcgtac acggtatggt ccaccgtccc ggccgcgatc
     1081 agcggcgaca cgacgatcgt aggcacccgg acgccgtact gctcgaaggt gaagccgtac
     1141 gcgttgaaac cgtcgcctgg atggtcgccc ggcgcggtcg ccgtacccgg cgcgacggag
     1201 tcgtagaatc cgccatgctc gtcgaacgtg atgatcagca agctgctgtt ccagtacggt
     1261 gagttccgga tcgcttcgta gacggcctgc accagtttct gaccacccgc catgtcatcc
     1321 accggatgct gggacgagcc gcctgtgtag cttccgcgca cgatatcgcc gtagtggggc
     1381 tcgatgaacg tgtaggtata gggatagggg ccttgcaggt cggacgcgaa gtgcaacagg
     1441 gaatggaaat ccatcaagga cacgccttgc agtgacgagg cctgcgggat ggcgccggcc
     1501 agcgacccgt tctgcacatc atcgccgaac aggctcagac cgtacgagga tcccggcgac
     1561 gggttgaaat cgctgtaaag tcgatactgg atactggcat cgtccagcgc ctggaagatc
     1621 gatccatggg gaaacggaaa gccatcgatc tcccacgttc ccatctgcac cagggacgga
     1681 ctatcatcca ggccggacga cgaggcgccg tggacgaagt agcggttggg ccaggtcggc
     1741 ccgggtagcg aagagaacca ctggtcgcac agcgcgaact gcgtggccag ctggtagaac
     1801 acggggagct ggctctgcgt cccgaaacac gccatgatgt cgccgatgtc ttgcggcggt
     1861 gaagcggggc cttccgtggt cgaagtggca tagtttgccg caaatccgga gttgttgatc
     1921 gccggatacg ggccacccgg ggggtacacg gcgccctcgc cgccgagctg ctcgacgaca
     1981 tcgagaaact cgtgacctgg atcggtcggc atgacgaccg gcgcgccgtt ctgcacgtgg
     2041 taggcggtgt cgtcatggga gttggagtcg cgggtggtcg ccgcgatgat gcccgggatc
     2101 cccgacatcg cgaacatgcg gtcgaacgac tggttctcca gcatcacgac gaacacgttc
     2161 gtgatcggcg agtacggcgc aagcttcgtc atcccgcccg tgcatccgcc ggagtcgcgc
     2221 gcgacctcga aggtggtcgt gtcgaccgac agggtgatgg tcttgccggc gtcgccgtgt
     2281 tgcagctggc actccttcca gtacggaacg atcgcggtcc cgctggtgat gtactgcccc
     2341 ggtgtcgacc ctccgctcac gttgagcatc acggaccagt aatccaggat tccgccggtg
     2401 ccgagcccgg tctcgaaata gaccgtcatc gggcctacgg tctgaccggg accggcattc
     2461 cagctctcgc gctgtgtgcc atgactgga
//
LOCUS       DASVOR010001215         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420790,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001215 DASVOR010000000
VERSION     DASVOR010001215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420790"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            313..753
                     /locus_tag="VF516_23095"
     CDS             313..753
                     /locus_tag="VF516_23095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110645.1"
                     /translation="MLHGDAPARRVVRAPAQDDNLLLLTGGIPQTVNLSVVAGVPMFS
                     LPGSTQLYTGIKWEPNGYGLMITFAFQQPSGSSGPFISELAPITPSLWISDTFPEDSE
                     SNQMCCVPPTLGEYHFNVVLDDGTLALGDGTMVVDPRIVVTPIT"
     gene            complement(772..1713)
                     /locus_tag="VF516_23100"
     CDS             complement(772..1713)
                     /locus_tag="VF516_23100"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8110646.1"
                     /translation="MAILAKHPDGPHAHLLSRHLVGRSRLADLRMTDPTVSGEHAVLR
                     WTGREWELHDLGSRNGTSVDGRRLASGERVAIGRGTVIAFGQTDNAWRLDDDAPPTIM
                     AVFDDGDPPLTGRNDLLALPSDDDPDAVIYRDAGGNWVIEQGGETARIADRAAVRAGG
                     RDYVLRVPDMIAPTWDSSSPAPHLAMMTLQFSVSRDEEYVALTARGEHHVIDLGARAH
                     HAVLLSLARSRLADRKARGAAADRGAPPESSEGWVYQDDLARELAIDETHLNVAVFRC
                     RRQLAEAGIVGAASIVERRRPTRELRLGVARIEIVTV"
     gene            complement(1911..>2362)
                     /locus_tag="VF516_23105"
     CDS             complement(1911..>2362)
                     /locus_tag="VF516_23105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110647.1"
                     /translation="KPEPQPAPANEAKRPAARPPVNQQSTSAASNATNAQTERRLEKT
                     ANSANQGNAASGDAARQAQLLDWARKQRDQVIALVNANRCRDAASVAVDIYNRAPEYF
                     NANVATDRQIRPCLAYVNDQRDRAERSRASKNAADVQAPAQAAPARK"
BASE COUNT          303 a          840 c          856 g          363 t
ORIGIN      
        1 gagctcgccg gggcggcggc ggccggctac cgcggcctgg gccaccaatc gcaggaccag
       61 gtacgggaga tcgagcgatg gctggcggcg ccgtgacggc agggccgcgt gaaatgccgt
      121 gtaatcgtcc gttatggtaa tgccggacgg cgctgcctag agtggcccgg cacgcgagcc
      181 agggggatcg cgcggggcct cgaccggacg tgggggctcg cgtgcacacc cgtgtgcgga
      241 tccccgtgaa aggaccgaga gatgacgaat ctagcgcagc cagcaacgag cccgacgaac
      301 caccatcgac ccgtgctgca cggcgacgcg ccggcgcgac gggtggtccg cgccccggca
      361 caggacgaca acctgctcct gctgaccggc ggcataccgc agaccgtcaa cctgtccgtc
      421 gtcgccggcg tccccatgtt ctcgctaccg ggctcgacgc agctgtacac cgggatcaag
      481 tgggagccca acggctacgg gctgatgatc acgttcgcgt tccagcagcc gtcgggctcg
      541 tccgggccgt tcatcagcga gctggctccg atcacaccgt cgctctggat ctcggacacg
      601 tttccggagg acagcgagtc gaaccagatg tgctgcgtcc cccctactct cggagagtac
      661 cacttcaacg tcgtgctcga cgacggtacg ctggccctcg gcgacggtac gatggtcgtg
      721 gacccgcgga tcgtcgtcac gccgatcacc tgagcgccgt gccgtcgccg ttcacacggt
      781 gacgatctcg atccgcgcga cgccgagccg cagctcgcgg gtcggccggc ggcgctcgac
      841 gatgctcgcc gcgccgacga tgccggcctc ggcgagctgg cgccggcagc ggaacaccgc
      901 gacgttgagg tgggtctcgt cgatcgcgag ctcgcgggcc agatcgtcct ggtagaccca
      961 gccctccgag ctctccggcg gcgccccgcg atcggcggcg gccccgcggg ccttgcgatc
     1021 cgccagccgg ctgcgcgcca ggctgagcag caccgcgtgg tgggcgcgcg ccccgaggtc
     1081 gatcacgtgg tgctcgccac gcgcggtcag cgcgacgtac tcctcatcgc ggctgaccga
     1141 gaactgcagg gtcatcatcg ccaggtgcgg cgccggcgag ctcgagtccc aggtcggcgc
     1201 gatcatgtcg ggcacgcgga gcacgtagtc gcggccgccg gcgcgcaccg ccgcgcgatc
     1261 ggcgatccgc gcggtctcgc cgccctgctc gatcacccag ttgccgcccg cgtcgcggta
     1321 gatcacggcg tcggggtcgt cgtcgctcgg cagcgcgagc agatcgttgc ggccggtgag
     1381 cgggggatcg ccgtcgtcga acaccgccat gatcgtcggc ggcgcgtcgt cgtccagccg
     1441 ccacgcgttg tcggtctgac cgaacgcgat caccgtgccg cggccgatcg cgacccgctc
     1501 gccggacgcg agccggcggc catcgacgct cgtgccgttg cggctgccga ggtcgtggag
     1561 ctcccactcc cgtccggtcc agcgcagcac ggcgtgctcg ccggacaccg tcggatcggt
     1621 catccgcaga tcggccagtc gggaccggcc gaccaggtga cgcgacagca ggtgcgcgtg
     1681 aggtccatcg gggtgcttcg cgaggatcgc catgtcggat gaaacggctg atcctgcgcg
     1741 cgcgcggcga gcggatacaa gcctcgcgcg ccacgattcc acctgcgagc cgccgtgcgc
     1801 tgccggtgtc atgcgccacg tcggctatca gcgcgatcac acatggctcg cgctgcgacg
     1861 accggtggcg cgatgtgcgg cagagcgcag cggcggctgc ccgctgcgcg tcacttgcgt
     1921 gccggcgcgg cctgcgccgg cgcctggacg tcggccgcgt tcttgctcgc ccggctgcgc
     1981 tcggcgcgat cgcgctggtc gttgacgtac gccaggcacg gcctgatctg gcggtcggtg
     2041 gccacgttgg cgttgaagta ctcgggcgcg cggttgtaga tatcgaccgc gacgctggcg
     2101 gcatcgcggc agcggttggc gttgacgagc gcgatcacct ggtcgcgctg cttgcgggcc
     2161 cagtccagca gctgcgcttg cctggccgcg tcgccgctgg ccgcgttccc ctggttggcc
     2221 gagttcgccg tcttctccag ccggcgctcc gtctgcgcgt tcgtcgcgtt gctggccgcg
     2281 gatgtcgact gctggttgac cgggggcctg gcggccgggc gcttggcctc gttcgccggg
     2341 gctggctgcg gctcgggctt cg
//
LOCUS       DASVOR010001216         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_13138,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001216 DASVOR010000000
VERSION     DASVOR010001216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13138"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VF516_23110"
     CDS             complement(<1..783)
                     /locus_tag="VF516_23110"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8110648.1"
                     /translation="MSPLDYARHDGLGLAALVARGEVSPAELVESAIACIERHNGTLN
                     AVVHKAYDEARRTAAGKLPDGPFKGVPFLIKDLARRVAGWPCSMGSVFGQLPPAAEDS
                     VLVRRYRDAGLILLGATNTPEFGIPGVTHSQRLGLCKNPWNPAHIPGGSSGGSAAAVA
                     SGMVPMAHASDGLGSIRIPAACCGLVGLKPTRDRNPWDPDEQWRAGGLAVDHIVCRSV
                     RDCAAMLDATGTPQPASPYAPPPKAGPYLDEVGRAPGVLRIAW"
     gene            complement(937..1335)
                     /locus_tag="VF516_23115"
     CDS             complement(937..1335)
                     /locus_tag="VF516_23115"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II truncated hemoglobin"
                     /protein_id="HEX8110649.1"
                     /translation="MSEPQTPFALLGGADRVRALVESFYDLMSDREPALSRLHRCTPD
                     GRVDRYSRDRFALFLIGWLGGPEDYIAQHGHPRLRMRHARVPVSTAMRDAWLRCMTAA
                     MDAQQVAGPVRAFLDTRFAEVADAMRNVAE"
     gene            1500..1847
                     /locus_tag="VF516_23120"
     CDS             1500..1847
                     /locus_tag="VF516_23120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110650.1"
                     /translation="MCAALRPAVPTISTETELCEPLSDEVLTAVAAGADPGYNPADDA
                     LGRAGPGRSWSWLGNYYTPEALAHDQAVRGNLAAGSSKVMAHVKALPLLPAAVGSYFR
                     ARFHPGPDDMHLP"
     gene            complement(1872..>2166)
                     /locus_tag="VF516_23125"
     CDS             complement(1872..>2166)
                     /locus_tag="VF516_23125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110651.1"
                     /translation="AWRQRPGKSADDAGLLDRTQLAELAGWLTPEIHRDLGITEGADS
                     FIAASRAHRRRWWPSKTSIGGALAILLILMILVTPITLASLIALTAAMIHKFL"
BASE COUNT          279 a          754 c          856 g          277 t
ORIGIN      
        1 ccacgcgatg cgcagcacgc cgggcgcgcg cccgacctcg tcgaggtacg gcccggcctt
       61 gggcggcggc gcgtacgggc tcgcgggctg cggggtgccg gtggcgtcga gcatcgcggc
      121 gcagtcgcgc accgagcggc acacgatgtg gtcgaccgcg aggccgcccg cccgccactg
      181 ctcgtcgggg tcccacgggt tgcgatcgcg ggtgggcttg aggccgacca ggccgcagca
      241 cgcggcgggg atccggatcg agcccaggcc gtcgctggcg tgcgccatcg gcaccatccc
      301 cgaggcgacc gcggcggccg atccgccgga cgagccgccc gggatgtgcg ccgggttcca
      361 cgggttcttg cacaggccga ggcgctggga gtgggtgacg ccggggatgc cgaactcggg
      421 ggtgttggtc gcgccgagca ggatcaggcc ggcgtcgcga tagcgccgga ccagcacgct
      481 gtcctcggcg gcgggcggaa gctgcccgaa cacgctgccc atgctgcacg gccagccggc
      541 gacgcgccgg gccagatcct tgatgaggaa cggcacgccc ttgaacgggc cgtcgggcag
      601 cttgccggcg gcggtgcggc gcgcctcgtc gtaggccttg tgcacgaccg cgttgagcgt
      661 gccgttgtgg cgctcgatgc acgcgatcgc cgactcgacg agctcggccg gcgagacctc
      721 accgcgcgcg accagcgccg ccaggcccag gccgtcgtgg cgggcgtagt cgagcgggct
      781 catcgcggcc ccgcgggctg ggcgggcacg aggcccggcg cgttcgttcg gggttcgatc
      841 tggatgctcg gcatggcgac ctccgtgcgc atctgatgat ggccggtgcc ggaggtcaag
      901 cgtgcggagg cgctccggac cggggaatcc gttgcgctac tcggccacgt tgcgcatggc
      961 gtcggcgacc tcggcgaacc gggtgtcgag gaacgcgcgc accgggccgg cgacctgctg
     1021 ggcgtccatc gccgcggtca tgcagcgcag ccaggcgtcg cgcatcgcgg tgctgaccgg
     1081 cacgcgggcg tggcgcatgc ggagccgcgg gtggccgtgc tgcgcgatgt agtcctcggg
     1141 cccgccgagc cagccgatga ggaacagggc gaagcggtcg cgcgagtagc gatcgacgcg
     1201 gccgtcgggg gtgcagcggt gcagccgcga cagcgccggc tcgcgatcgc tcatgaggtc
     1261 gtagaagctc tcgacgagcg cgcgcacgcg atcggcgcct cccagcaagg cgaacggcgt
     1321 ctggggctcg gacacgccga cactgtgcgg cgcgtcgccg cgatgcgcaa tcggcgggtc
     1381 cgggaatgcg cgcagagccc gcacgcggcc caacgtgtcg acgccggtcg acgcgctgtc
     1441 gaccgcggtg cgcacgaggc cgtggccaac cgcgcgaacg gcgcggcgtt cgccatggca
     1501 tgtgcgctgc actgcgcccg gccgtgccga cgatctcgac ggagaccgag ctgtgcgagc
     1561 cactgtccga cgaggtgctg accgcggtcg cggccggtgc cgaccccggg tacaacccgg
     1621 ccgatgatgc gctcggccgg gccgggccgg gcaggagctg gagctggctc ggcaactact
     1681 acacgccgga ggcgctcgcc cacgaccagg cggtccgcgg caacctcgcc gcgggctcgt
     1741 cgaaggtgat ggcgcacgtc aaggcgctgc cgctcttgcc cgccgcggtc ggatcgtact
     1801 tccgggcccg gttccacccc gggccggacg acatgcacct cccgtgaccg cgcgcgttcg
     1861 ccgcgcgatc gtcacaggaa cttgtggatc atcgcggcgg tgagcgcgat gagcgacgcc
     1921 agggtgatcg gcgtcaccag gatcatcagg atcagcagga tcgcgagcgc gccgccgatc
     1981 gaggtcttgc tcggccacca gcgccggcgg tgggcgcggc tggcggcgat gaacgagtcg
     2041 gcgccctcgg tgatgccgag gtcgcggtgg atctccgggg tgagccagcc ggcgagctcg
     2101 gcgagctggg tccggtcgag caggccggcg tcgtcggcgc tcttgccggg gcgctgccgc
     2161 cacgcg
//
LOCUS       DASVOR010001217         1793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_538490,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001217 DASVOR010000000
VERSION     DASVOR010001217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538490"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1494
                     /locus_tag="VF516_23130"
     CDS             <1..1494
                     /locus_tag="VF516_23130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110652.1"
                     /translation="DPIAAIGGAFETAAAVAGPKTKTGEALTRGSKIAGFANAGKRAL
                     AARPPDYAAVSEAALGIAGQLHDDRRIDDASKIIASANRLKQAWAKRDSDPAGLAAAA
                     LDLAQAIQLAKYDADHDETKDADGKPAADPDRAAITERYQRAGRIVGAAKAALTAATA
                     KPRPNYLAALDAVSQLAAELTASKQVDAAALVTAKLDAWTQAVNSKNEAAIIAAGTEL
                     GEAISGLRGAIAGEHDAAKQAAQSNLAPGETLPDDGGAELPPGPSAAHDGDIVDGDAI
                     DRDAPADATADPGDTTGGPGDAIGGPSDGGYAITPVAYQPDPQAPNKPAPPRQPSISG
                     KIVAGGPLSNAEVAAIDEAAKKVPSQFRRYIAYAGVIKVGGSLSWRANNPGNLRDAPT
                     KIDTVSGAVGTFAVFATMDDGRAAQRSLYLDKYGDQTVRDAINKLTPPSENDTTAYLA
                     KLEAAGVDLDKDVKSQIDVLMPAVEANEGMITGIEVQRQADAQGMRA"
     gene            complement(1543..>1793)
                     /locus_tag="VF516_23135"
     CDS             complement(1543..>1793)
                     /locus_tag="VF516_23135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110653.1"
                     /translation="IVGINTNFGDIAIIRNVTLNNSGTSKTKICQVYKGVVKGQGSTS
                     ALGVEFNTPNCQVSQSDVTLLPPSQMNTAACAGSCPIP"
BASE COUNT          298 a          672 c          618 g          205 t
ORIGIN      
        1 gatccgatcg ccgcgatcgg cggcgcgttc gaaaccgcgg cggccgtcgc cgggcccaag
       61 accaagaccg gcgaggcgct gacccgcggc tcgaagatcg ccggcttcgc caacgccggc
      121 aagcgcgcgc tcgcggccag gccgcccgac tacgccgcgg tcagcgaggc cgcgctcggc
      181 atcgccggcc agctccacga cgaccgccgg atcgacgatg cgagcaagat catcgcgtcg
      241 gccaaccggc tcaagcaggc ctgggccaag cgcgactccg atccggccgg cctcgccgcc
      301 gccgcgctcg acctcgccca ggcgatccag ctcgccaagt acgacgccga ccacgacgag
      361 accaaggacg ccgacggcaa gccggccgcc gaccccgatc gcgcggcgat caccgagcgc
      421 taccagcgcg ccggacggat cgtgggtgcg gccaaggcgg cgctgaccgc ggcgaccgcg
      481 aagccgcggc ccaactacct cgccgcgctc gatgccgtgt cgcagctcgc cgccgagctc
      541 accgcgagca agcaggtcga cgccgcggcg ctggtgaccg ccaagctcga cgcgtggacc
      601 caggccgtga acagcaagaa cgaggccgcg atcatcgcgg ccgggaccga gctgggcgag
      661 gcgatctccg ggctgcgcgg cgcgatcgcc ggcgagcacg acgccgccaa gcaggccgcc
      721 cagtccaacc tcgcaccggg cgagacgttg cccgacgacg gcggcgccga gctgccaccc
      781 gggccgtccg ccgcgcacga cggcgacatc gtcgacggtg atgcgatcga ccgcgacgcg
      841 ccggccgacg ccaccgcgga ccccggcgac accaccgggg gccccggcga cgccatcggg
      901 ggccccagcg acggcggcta tgcgatcacg cccgtggcct accagcccga tccgcaggcg
      961 ccgaacaagc ccgcgccgcc caggcagccg tcgatctccg gaaagatcgt cgcgggcggt
     1021 ccgctcagca acgccgaggt cgccgcgatc gatgaagccg ccaagaaggt gccgtcgcag
     1081 ttccggcgct acatcgccta cgccggcgtg atcaaggtcg gcggctcgct gtcgtggcgc
     1141 gccaacaacc cgggcaacct gcgcgacgcg ccgaccaaga tcgacacggt gtccggcgcg
     1201 gtcggcacgt tcgccgtgtt cgcgacgatg gacgacggcc gcgctgcgca gcgctccctg
     1261 tacctcgaca agtacggcga tcagaccgtc agggacgcga tcaacaagct gacgccgccg
     1321 tcggagaacg acaccacggc ctacctcgcg aagctcgagg cggccggcgt cgatctcgac
     1381 aaggacgtca agtcgcagat cgacgtgctg atgccggcgg tcgaggccaa cgagggcatg
     1441 atcaccggca tcgaggtgca gcgccaggcc gacgcgcaag gcatgcgcgc ctgaccgcag
     1501 cgccgccggt gccgtgcacc gcgtccgtgc gcggcgacct cgctacggaa tcggacagga
     1561 gcccgcgcag gcggcagtgt tcatctggct cggcggcagc aacgtgacat cgctctggct
     1621 gacctggcag ttcggcgtgt tgaactccac ccccagggcg ctggtgctgc cctggccctt
     1681 caccacgccc ttgtagacct ggcagatctt ggtcttgctg gtgccgctgt tgttgagggt
     1741 cacgttgcgg atgatggcga tgtcgccgaa gttggtgttg atcccgacga tcg
//
LOCUS       DASVOR010001218         2443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_13173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001218 DASVOR010000000
VERSION     DASVOR010001218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1541
                     /locus_tag="VF516_23140"
     CDS             <1..1541
                     /locus_tag="VF516_23140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110654.1"
                     /translation="PDAGNVSLVLDIPNGALDPQGYTSAEIVLHEPTGDVTRTASVDM
                     NGNFNLDRIDPSTSVSVEAKLRNASGAAVGSGRTAVASALAAGAQITVPVRRPIAYIA
                     GTVSRDADGNPSTPALHWTEVPATFSDLSAGTGIDGHAVVGSNVVLAVSAGPSLYMIT
                     QATMDPNGALTGPAKIMPISAADHSVGAALSGSMTGAVIDGAGSDDGSALVIGTATEL
                     FAIDTASGAARVLTDGNFARVAIVAAASGELDALAIKNRPSTTATTCATTAELWWVPI
                     TPGAATAPHIVATGGFADVAADRGHAYYVDACKGELGEVTASATQMLRTLPGTGPGAT
                     APVGKPTALAVSNGQAYIGIEIPPASSSAPASTSLLVASVASSDPPRTLWTEGARQVL
                     DVMSQPDVQRQLSASSVVIPHLEVGAGGDYIALTTSAHFHGAEIPQLFPDMTIDAEEL
                     RVFAAATGGVIQRYRSWCDGVLIIGFNEISGWECSAATGQSAAASDALEHHIGSMTFL
                     FGKK"
     gene            1538..2407
                     /locus_tag="VF516_23145"
     CDS             1538..2407
                     /locus_tag="VF516_23145"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8110655.1"
                     /translation="MMRPTCRAALSWLCVAAVLAAAPRAHADPDPKRRVSVLEYRAGS
                     SALPGIAARVATTLSRKTSLGVLGQDQTRAIYGDQLDRALVKCAGEVACVARIGQKVG
                     AAEIILVGISELGDVILTMQRIDVAGREVTGRVADSLAAGSAPTDDQILGYANRLLPP
                     SDFLRFGVIDIAANLPGAAVTIGGAARGLTPIEALHLPAPANYDIRIEKAGYVPFTTR
                     VALPPDGEIKVEAELVWARRGAAWYQHWYVLAAAGVIVAGAGGTALYFATRTTTTPAP
                     SGDGTLHVTGSIH"
BASE COUNT          349 a          924 c          858 g          312 t
ORIGIN      
        1 ctccggacgc cggcaacgtc tcgctcgtgc tcgacatccc caacggcgcg ctcgaccccc
       61 aggggtacac ctccgcggag atcgtgctgc acgagccgac cggcgacgtc acccggactg
      121 ccagcgtcga catgaacggc aacttcaacc tcgaccgcat cgacccgagc accagcgtat
      181 cggtcgaagc gaagctgcgc aacgcgagcg gcgcggcggt cggctccggc cggacggctg
      241 tggcatccgc gctcgccgca ggtgcgcaga tcaccgtgcc ggtgcggcgc ccgatcgcct
      301 acatcgccgg aacggtgagc cgcgatgccg acggcaaccc cagcacgccg gcgctgcact
      361 ggaccgaggt gccggcgacg ttctccgacc tgtcggcagg taccggcatc gacgggcacg
      421 cggtggtcgg tagcaacgtc gtgctcgcgg tctccgcggg gccgagcctg tacatgatca
      481 cccaggcgac catggatccg aacggcgcgc tgaccgggcc cgccaagatc atgccgatct
      541 ccgccgcgga tcactcggtg ggcgccgcgc tgtcgggctc gatgaccggc gcggtgatcg
      601 acggcgccgg gtccgacgac ggctcggcgc tggtgatcgg gacggccacc gagctgttcg
      661 cgatcgacac cgcgagcggc gcggcgcgcg tgctgaccga cggcaacttc gcgcgcgtgg
      721 cgatcgtcgc cgccgcgagc ggcgagctcg acgccctcgc gatcaagaac cggccgtcga
      781 ccaccgcgac gacctgcgcg accaccgcgg agctgtggtg ggtgccgatc accccgggcg
      841 cggccaccgc gccgcacatc gtcgcgaccg gcggattcgc cgacgtcgcg gccgatcgcg
      901 gccacgcgta ctacgtcgac gcctgcaagg gcgagctcgg cgaggtcacc gcctccgcga
      961 cccagatgct gcgcaccctg ccgggcaccg ggcccggcgc gaccgcgccg gtgggcaagc
     1021 cgaccgcgct cgcggtgtcc aacggccagg cgtacatcgg catcgagatc cctccggcga
     1081 gcagctcggc cccggccagc acgtcgctgc tggtcgcatc ggtcgcgagc tccgacccgc
     1141 cgcgcacgct gtggaccgaa ggcgcgcgcc aggtgctcga cgtcatgagc cagcccgacg
     1201 tccagcgcca gctcagcgcg agctcggtgg tgatcccgca cctcgaggtc ggcgcgggcg
     1261 gcgactacat cgcgctgacc accagcgcgc acttccacgg cgccgagatc ccgcagctgt
     1321 tccccgacat gacgatcgac gcggaggagc tccgggtgtt cgccgcggcg accggcggcg
     1381 tgatccagcg ctaccgcagc tggtgcgatg gcgtcctgat catcggcttc aacgagatca
     1441 gcggctggga gtgctcggcg gccaccggcc agagcgccgc cgcgagcgac gcgctcgagc
     1501 accatatcgg ctcgatgacc ttcctgttcg gaaagaaatg atgcgcccca cctgccgcgc
     1561 cgcgctgtcc tggctgtgcg tcgccgcggt gctcgccgcc gcgccgcgcg cccacgccga
     1621 tcccgatccg aagcgccggg tcagcgtgct ggagtaccgc gccggctcgt cggcgctgcc
     1681 cgggatcgcc gcgcgcgtcg cgaccacgct gtcgcgcaag acctcgctcg gcgtgctcgg
     1741 ccaggaccag acccgcgcga tctacggcga ccagctcgac cgcgcgctgg tcaagtgcgc
     1801 cggcgaggtc gcgtgcgtcg cgcggatcgg ccagaaggtc ggggccgccg agatcatcct
     1861 cgtcgggatc agcgagctcg gcgacgtgat cctcacgatg cagcggatcg acgtcgccgg
     1921 ccgcgaggtc accggccggg tcgccgactc gctcgccgcc ggaagcgcgc cgaccgacga
     1981 ccagatcctg ggctacgcca accggctgtt gccgccgagc gacttcctgc ggttcggcgt
     2041 gatcgacatc gccgccaacc tgcccggcgc ggcggtgacg atcggcggcg cagcgcgcgg
     2101 gctgaccccg atcgaggcgc tccacctgcc ggcgccggcg aactacgaca tccggatcga
     2161 gaaggccggc tacgtgccgt tcacgacccg ggtcgcgctg ccgccggacg gcgagatcaa
     2221 ggtcgaggcc gagctcgtgt gggcgcggcg cggcgcggcg tggtaccagc actggtacgt
     2281 gctcgccgcc gcgggcgtga tcgtcgccgg cgcgggcggg accgcgctgt acttcgcgac
     2341 ccgcaccacc acgaccccgg cgccgtcggg cgacggcacg ctccacgtca caggctccat
     2401 tcactgatca tcacgagcgc gtcctcgccg ttgtgctggt agt
//
LOCUS       DASVOR010001219         5712 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_217020,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001219 DASVOR010000000
VERSION     DASVOR010001219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5712
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217020"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1650
                     /locus_tag="VF516_23150"
     CDS             <1..1650
                     /locus_tag="VF516_23150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110656.1"
                     /translation="LALAGLGGVVTWRQRVLWRRRALQLTQQSDARFRELIEAMPDLV
                     SVHRDDQLIYLNQAARRMLGADATRTPRQVDLVERIHPDDRPRAETLFSDAQTSEGQS
                     HGHPVELRLAAGDGSWRRCELSGRRIDLGDGPVVVVTGRDVTERDRLRAKLLLSDRMA
                     SLGTLAAGIAHEINNPLAYVTANLEVVAESLAAAPPAPSPEQRAEHEEMQAAIGDARE
                     GAERVRKIVRGLRSFSRSEEEKRVPIAVSDVLAAAIRLTSNEVKHRAVLVLDLAPTPA
                     VLADDGRLAQVFINLLINAAHAIPEGNTDANRITVRTRTALDGRAITEIEDTGAGMPP
                     EVLARAFDPFFTTKGIGEGTGLGLSICHGIISGLGGQITIDSAPGRGCLVRVVLPPAD
                     PAAEPALPPVPTTTDISPRRRRVLIVDDEPKVAQALERMLNTDYDLTLVSCGATAIEH
                     ITAGVRYDAIITDVMMPNMTGVELFDRLEALAPDQASRVIFLTGGVFTPQTQTRLEAA
                     GNPQLQKPVGAQELRACIAKLFASPRRAERVTAAPARDRAG"
     gene            2316..3686
                     /locus_tag="VF516_23155"
     CDS             2316..3686
                     /locus_tag="VF516_23155"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110657.1"
                     /translation="MHLQLQQPVAMKFLLPDVISNQQVVQRFLREAQAAVRLRSEHVA
                     RVIDVGALETGAPYMVLEYLEGGDLSSFPRSQLTVGGIVDLVLQACEALAEAHSLGIV
                     HRDIKPANCFITRRADGTLLLKVLDFGISKISTMNGQLTATQAVMGTPAYMSPEQMRS
                     SRDVDHRSDIWSLGVVLYELLQGAPPFGGDTFSSMVLKVVNDPLPRLTVRLPGDLENI
                     VYRCLEKDPAKRFQNTAELAHALARYAQSETQAAISVQRTRGIVGLQAPHAAFETDPS
                     GRAFPSTISGAAGARTMPRRRGRRWPTLAVGLLAGAIVVAIAAVNGGGKTEDRSPSAQ
                     PEGPPSTAPPPPPEVSRDSAIPAVPASSPAVAVPTSTAPPVEAAPPPAPAPVATPPPA
                     RPTPTTASSESPTSTTPSSTAAKTSKKHTKASVSPAKAGSSEKRQAPPAKSPSPADDD
                     VLDTRN"
     gene            3731..4783
                     /locus_tag="VF516_23160"
     CDS             3731..4783
                     /locus_tag="VF516_23160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8110658.1"
                     /translation="MSVLAGGAHAQVKNSSTIAEQLFKEARDLAKANHWAEACTKFEA
                     SLHHEPVLGTRLNLATCYEHIGKLASAWSIYRESIALAKKANDAKRAAYAQKHADALE
                     PRLAKLVISAPATQPAGFVVTRDGTPLDGSALGIALYVDAGPHGIAASAPGFEAFTHT
                     VTLSDGKTETVEIPTLAAIPAPAPEAPKPAPEAPKPGTQDHAATTEPVVVTSPPHSSA
                     TITPATESAPAARVPSTRKYVGIGVGAAGVAATGIGLLFGAKASSANRDAKSLCGADL
                     VCSNSADYEKGKQLLGDAHSNATISTVLVAAGGAAVVAGAIVLLTAHPTAERTVARIV
                     PVSHDRGAVLAVVGRF"
     gene            4786..>5712
                     /locus_tag="VF516_23165"
     CDS             4786..>5712
                     /locus_tag="VF516_23165"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HEX8110659.1"
                     /translation="MRCAALLVTLVTANAGCSQCLGLSEVSLGEPARLIAPLSMSTVT
                     QQRPLLRWQLSTVQHELIVDLCKDRSCMSPLPVTAQVSGDHVSAVPSAPLPAGWVFWR
                     VRTGSGAHMVSSATWQFWVGKSSASNPVDTSNGAILDVNGDGYPDLLIGADSAGAGAG
                     TAHLYLGSATASVTEWNAASTATRIDLISPDSANASFGYSVSSAGDINGDGFADFLIG
                     APGAGANSGTAHLYFGSAKPSADDWNGTSARRRIDLDDLAGEGSVFGASVASAGDVNG
                     DGFADFLVGAAKAGMAHLYLGSATPGAEDWSGT"
BASE COUNT          930 a         2048 c         1882 g          852 t
ORIGIN      
        1 ctcgcgctgg ccggcctggg cggcgtggtg acctggcggc agcgcgtgct gtggcggcgc
       61 cgcgccctgc agctcaccca gcagtccgac gcccggttcc gcgagctgat cgaggcgatg
      121 cccgacctcg tgtcggtcca ccgcgacgac cagctgatct acctcaacca ggcggcgcgg
      181 cgaatgctgg gcgccgacgc gacccgcacg ccgcgccagg tcgacctggt cgagcggatc
      241 caccccgacg atcgcccgcg cgccgaaacc ctgttttccg acgcccagac ctcggagggt
      301 cagtcccacg gccacccggt cgagctgcgg ctggccgccg gcgacggcag ctggcgccgc
      361 tgcgagctgt ccggccgccg gatcgatctc ggcgacggcc cggtcgtcgt ggtcaccggc
      421 cgcgacgtca ccgagcgcga tcggctgcgc gccaagctgc tgctgtccga ccgcatggcg
      481 agcctcggta cgctggccgc cggcatcgcg cacgagatca acaacccgct cgcctacgtc
      541 accgcgaacc tcgaggtcgt cgccgagtcg ctcgccgccg cgccgcccgc gccctcgccg
      601 gaacagcgcg ccgagcacga ggagatgcag gccgcgatcg gcgatgcccg cgagggcgcc
      661 gagcgcgtcc gcaagatcgt gcgtggcctg cgcagcttca gccgctccga ggaggagaag
      721 cgcgtgccga tcgcggtgtc cgacgtgctc gccgccgcga tccggctgac cagcaacgag
      781 gtcaagcacc gcgcggtcct cgtgctcgac ctcgcgccga cccccgcggt gctggccgac
      841 gacggccggc tcgcccaggt gttcatcaac ctcctgatca acgccgccca cgcgatcccc
      901 gagggcaaca ccgacgccaa ccggatcacc gtgcggaccc ggaccgcgct cgacggccgg
      961 gcgatcaccg agatcgagga caccggcgcc gggatgccgc ccgaggtcct ggcccgcgcg
     1021 ttcgatccgt tcttcaccac caagggcatc ggcgagggca ccgggctcgg gctgtcgatc
     1081 tgccacggca tcatcagcgg gctcggcggc cagatcacga tcgacagcgc gccgggccgc
     1141 ggctgcctgg tccgcgtcgt gctgccgccg gccgaccccg cggccgagcc ggcgctgccg
     1201 cccgtcccga cgaccaccga catctcgccg cgccgccgcc gcgtgctgat cgtcgatgac
     1261 gagccgaagg tcgcgcaggc tctcgagcgc atgctgaaca ccgactacga cctgaccctg
     1321 gtgtcgtgcg gcgccaccgc gatcgagcac atcaccgccg gcgtccgcta cgacgcgatc
     1381 atcaccgacg tgatgatgcc caacatgacc ggcgtcgagc tgttcgaccg gctcgaggcc
     1441 ctcgcccccg atcaggccag ccgcgtgatc ttcctgaccg gcggcgtgtt cacgccgcag
     1501 acccagacgc gcctggaggc ggccggcaac ccgcagctcc agaagccggt cggcgcccag
     1561 gagctccgcg cgtgcatcgc caagctgttc gccagcccgc gccgcgccga gcgagtcacc
     1621 gcggcgccgg cccgggaccg cgcggggtag cagctcgccg cacgtaccgc agtcgcggtc
     1681 cgggtcgcga ccgtcgtcgt ctgcggggcc agcggaacac cgtccgcgcc tcggacactc
     1741 atgtccggat atcggcaagc cggcacctgc gtcactcaca cgacacatac acctcctgca
     1801 ctgtcctcgc atatcggccg actggcttct ggtgccatcc ggaggcggtc cggttccagt
     1861 ttggcggtaa gcaccctgat tcgctatcac gggaccgaca ccccgcagca gtttgcactc
     1921 gcctccgctg aatactcacc cagtggacac ttcttcccga gtgatgaatc gcccgggtgt
     1981 tgtcgattcc aaacagatga acggccactc cagcgtgcgg cgaattgaat cgtggctgta
     2041 cttcgaattg tgctcgcttc cgatttactt ggttcggtcg gaggcataat gtcccgcgtg
     2101 acatgagcgt ggaggacgga cgttctccgt cacccgagga agtgcgtcgt ggataacgtc
     2161 atgggagcgc gaacgatcgt ccgtacggcc gcgtacgacg gaaggcagcg ggtgtgaccg
     2221 accagggtgc agtccagatt ggctccatcc tcgcgggccg gtatcgcatc gagcgagtgc
     2281 tcggccaggg cggcatggga atcgtcgtgc aggcaatgca tctccagctt cagcagcccg
     2341 tggcgatgaa gttcctgctg ccggacgtga tcagcaacca gcaggtcgtc cagcgattct
     2401 tgcgagaagc acaggcagcc gtccggttga ggagtgaaca cgtcgcacgc gtgatcgacg
     2461 tcggcgcgct ggagaccggg gcaccctaca tggtcctcga gtacctcgaa ggcggagatc
     2521 tctcgagctt tccccggtcg cagctgacgg tcggcgggat cgtcgatctg gtgctgcaag
     2581 catgcgaggc cctggccgag gcgcactccc ttggtatcgt gcatcgcgac atcaagccgg
     2641 cgaactgttt catcacgcgg cgggctgatg gaacgctgct gctcaaggtc ctcgacttcg
     2701 gaatctcgaa gatctccacg atgaacggcc agctcaccgc cacccaggcg gtgatgggga
     2761 caccggccta catgtcgccc gagcagatgc gctcgtcgcg cgacgtcgac caccgcagcg
     2821 atatctggtc gctcggcgtc gtgctctacg agctgctgca aggcgctccg ccgttcgggg
     2881 gcgacacgtt ctcttcgatg gtgctcaagg tcgtgaacga cccgcttccc agactcacgg
     2941 ttcggttgcc tggtgatctc gagaacatcg tctatcgctg tctcgagaag gacccggcga
     3001 agcgattcca gaacaccgcg gagctcgcac acgcgctcgc gaggtacgcg cagtccgaaa
     3061 cgcaggctgc gatctccgtg caacgtaccc ggggtatcgt cgggttgcag gcaccccacg
     3121 ctgcgttcga gaccgatccc tccggccgtg ctttcccgtc gaccatctcg ggcgctgcag
     3181 gtgccaggac gatgccacga cgccgtgggc ggcgctggcc gaccctggcg gtgggcctgc
     3241 tcgccggcgc catcgtggtc gcgatcgcgg ccgtgaatgg cggtggcaag accgaggatc
     3301 gatcaccgag cgcgcagccc gagggtccgc cgagcacagc gcctccaccg ccgcccgagg
     3361 tatccaggga ctcggccatc ccggccgtcc ccgcgtcgtc gccggccgtc gctgtaccga
     3421 cctccaccgc accgccggtc gaggccgcgc cgcccccagc gccggcaccg gtcgcgaccc
     3481 caccacccgc acgtcccaca ccgacgaccg cctcctcgga atcgccgacg tcgacaacgc
     3541 catcgagcac ggccgccaag acgtcgaaaa aacacacgaa ggcgtcggtg tctcccgcga
     3601 aggcgggatc ttctgagaag cggcaagcgc ccccggccaa gtctccctcg ccggcggacg
     3661 acgatgtcct cgacacacgc aactgagcgc aaggagagtc atgcgacatg cacttggcat
     3721 ggtcatggtg atgtcggtcc tggccggggg tgcgcatgcc caggtcaaga actcgagcac
     3781 gatcgccgag cagctgttca aggaggcacg cgatctcgcc aaggcaaacc attgggccga
     3841 ggcatgcacc aagttcgagg cgagcctgca ccacgagccc gtgctcggaa cccgcctcaa
     3901 tctcgccacg tgttacgagc acatcggcaa gctggcaagc gcgtggagca tctaccgcga
     3961 gtcgatcgct ctcgcaaaga aggccaacga cgccaagcga gccgcctatg cacagaagca
     4021 cgcagacgcg ctcgaaccgc ggctcgccaa gctcgtgatc tccgcaccgg ccacgcagcc
     4081 cgccggtttc gtcgtgacgc gggacggtac gccgctcgac gggagcgcac ttgggatcgc
     4141 gctctacgtc gatgccgggc cgcacgggat cgctgcgtcg gcgccgggtt tcgaggcatt
     4201 cacgcacacc gtcacgctca gcgatggcaa gacggagacg gtcgagatcc cgaccctcgc
     4261 ggccattccc gcgccggcgc cggaggcccc caagcctgcg ccggaggccc ccaagcctgg
     4321 gacccaagac catgccgcga cgaccgagcc cgtggtcgta acatcgccgc cgcacagctc
     4381 cgcgacgatc acgcccgcca ccgaatctgc ccctgccgcg cgggtgcctt cgacccggaa
     4441 gtacgtcggg atcggtgtcg gtgccgcagg cgtcgcagcc acggggatcg ggctcctgtt
     4501 cggggccaag gcgagctcgg cgaaccgcga tgcgaagtcg ctgtgcggtg cggacctggt
     4561 gtgcagcaac tccgccgact acgagaaggg caagcagttg ctcggtgacg cgcactcgaa
     4621 cgccacgatc tcgacggtcc tggtcgctgc gggcggcgct gcggtggtcg cgggcgccat
     4681 cgtgcttctc accgcgcacc ctaccgccga acggacggta gcgcggatcg taccggtcag
     4741 ccatgaccgg ggcgcagtcc tcgcggtcgt cgggaggttc tagtcatgcg ctgtgccgca
     4801 ctgctggtca ctctcgtcac ggcaaacgca ggttgctccc agtgcctggg attgagcgag
     4861 gtctccctcg gagaacctgc acgactcatc gcgccgctgt cgatgtcgac ggtgacccag
     4921 cagagaccgc tgcttcgctg gcagctcagc acggtgcagc acgagctcat cgtcgatctc
     4981 tgcaaggacc gatcgtgcat gtctccactg ccggtcaccg cacaggtttc cggcgaccat
     5041 gtcagtgcgg tgccgtcagc gccactgccg gcggggtggg tgttctggcg ggtacggacc
     5101 ggctcgggcg cacacatggt gagcagcgcg acatggcagt tctgggtggg caagtcgagc
     5161 gccagtaacc cggtcgacac cagcaacggt gcgatcctcg atgtcaacgg cgatggctac
     5221 cccgacctgc tcatcggcgc cgatagcgct ggcgcggggg caggcacggc ccatctctat
     5281 ctcggctcgg ccacggccag cgtgaccgag tggaacgccg cctccacggc cacgcggatc
     5341 gacctgatca gccccgacag cgcgaacgcc tccttcgggt attcggtgtc gagcgcaggt
     5401 gatatcaacg gcgacggttt cgccgatttc ctgatcggcg ctccaggtgc cggcgcgaat
     5461 tccggaacgg cccacctcta cttcggctcg gcgaagccga gcgccgatga ctggaacggg
     5521 acctcggcgc gcaggcgcat cgacctcgac gacctcgcag gcgagggctc cgtctttggc
     5581 gcctcggtgg cgagcgcggg ggacgtcaac ggggacggct tcgccgactt cctcgtcggc
     5641 gctgcgaagg cgggcatggc gcacctgtat ctcggatccg cgacgcccgg tgcggaggac
     5701 tggagtggga cc
//
LOCUS       DASVOR010001220         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_450624,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001220 DASVOR010000000
VERSION     DASVOR010001220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450624"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VF516_23170"
     CDS             complement(<1..285)
                     /locus_tag="VF516_23170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110660.1"
                     /translation="MSAKAAQRSSRALDVEASIDAIEGDAELAAEARRPDSATYLSMY
                     FRDMAALDVLRPEEEFTSAREIEALELLLWEAVLAHAPAVEHVLAAVEPIA"
     gene            complement(620..>1575)
                     /locus_tag="VF516_23175"
     CDS             complement(620..>1575)
                     /locus_tag="VF516_23175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8110661.1"
                     /translation="TLRDGGHGQPPPPPKAPPSPPPAAVKKDRPPPAAAPLAKPEARP
                     EARDVRDAKDAKDAKPDGKPRGKPWFVDLFDEDYLRTLPFLTPQATQAEAEFVSNALG
                     LQPGAQVLDVGCGYGRHAMELAARGFHIVGLDLSTPLLVRGGEEAHRRGLTIHFLRED
                     MRELDFDAQFDGAYCLFSTFGYFDDETNKKTVANMARALRPGGRLLIEILNRDYVIAD
                     LPTRVWWEGDGCVVLEEVELNYFSSRIHVNRSVVFDDGRQLEQEISVRAYSLHEVGKL
                     MHSAGLRVLEVSGAYHTRGRFFGNQSRHIIVLAERKDPAAG"
BASE COUNT          234 a          558 c          535 g          248 t
ORIGIN      
        1 cgcgatcggc tcgaccgccg cgagcacgtg ctcgaccgcc ggggcgtgcg ccagcacggc
       61 ttcccacagc aggagctcga gcgcctcgat ctcgcgcgcc gaggtgaact cctcctcggg
      121 gcgcagcacg tcgagcgccg ccatgtcgcg gaagtacatc gacaggtagg tcgcgctgtc
      181 cggccgccgg gcctcggccg cgagctcggc gtcgccctcg atcgcgtcga tcgaagcctc
      241 gacgtcgagc gcccgcgacg agcgctgcgc tgccttcgcc gacacgcgcg caactcgatt
      301 cttgcgcgcg ctcggacgac cgagaccctg ctgcccgacc ctggtgacga tggctgccat
      361 gaactgccct tctgcggaga tccgctggct cacccaattc agacgcgtgt gccgctccga
      421 tgttttggga ttcctacgcg gccggcatgg tggactaccc ccgaacctct cccgttattc
      481 caccggtccc gggcgtggat cccggcaagc ttttctagtg atattgaatc tttgaccagt
      541 gtgacctggc caacgtcgaa gcgaagaaag cggcggcggc gcggcccccc agagccccgc
      601 cccgcaaggt ccggaggggc tagcccgcgg ccggatcctt gcgttccgct aggacaatga
      661 tgtggcgcga ctggttgccg aaaaaacgac cgcgcgtgtg ataagcgccc gagacctcca
      721 gcacgcgcag gccggcggag tgcatcagct tgccgacctc gtgcaacgag tacgctctca
      781 cagaaatttc ctgctccagc tggcgcccgt catcgaacac caccgaccgg ttcacatgga
      841 tccgcgacga gaagtagttg agctcgacct cttccagcac cacgcagccg tcgccctccc
      901 accacacccg ggtcggtaga tcggcgatca cgtagtcgcg gttgaggatc tcgatcagca
      961 ggcggccgcc cgggcgcagc gcgcgtgcca tgttggcaac cgtcttcttg ttggtctcgt
     1021 cgtcgaagta gccgaacgtc gagaacaggc agtacgcgcc gtcgaactgg gcgtcgaagt
     1081 cgagctcgcg catgtcctcg cgcaggaagt ggatcgtcag gccgcggcgg tgggcctcct
     1141 cgccgccgcg caccagcagc ggggtcgaca ggtcgaggcc gacgatgtgg aagccgcgcg
     1201 ccgccagctc catggcgtgg cggccgtagc cgcagccgac gtcgagcacc tgcgcgccgg
     1261 gctggaggcc gagcgcgttg ctcacgaact cggcctcggc ctgggtcgcc tgcggggtca
     1321 gaaacggcag cgtgcgcagg tagtcctcgt cgaacaggtc gacgaaccac ggcttgccgc
     1381 gtggcttgcc gtcgggcttg gcgtccttgg cgtccttggc gtcgcggaca tcccgcgcct
     1441 ccggccgcgc ctccggcttg gccagcggtg cagcggccgg cggcggccgg tccttcttga
     1501 ccgccgccgg cggaggcgac ggcggggcct tggggggcgg cggcggctgg ccatgcccgc
     1561 cgtcgcgcag ggtcc
//
LOCUS       DASVOR010001221         1603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_304749,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001221 DASVOR010000000
VERSION     DASVOR010001221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304749"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF516_23180"
     CDS             complement(<1..375)
                     /locus_tag="VF516_23180"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8110662.1"
                     /translation="MSQRREALLARYRAISLDRLKSIRAKLTAVADGNTDALGEVRRE
                     LHTLKGDSHLLGLVQIGKVAHACEERLLDPRTLGSAVRVLGDALEAIKLALRDDAPEP
                     AAENGLRAALDQLAAQPPEIAAQ"
     gene            complement(372..>1603)
                     /locus_tag="VF516_23185"
     CDS             complement(372..>1603)
                     /locus_tag="VF516_23185"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEX8110663.1"
                     /translation="LTPPLQDWLHATTAGDGAITSPRDAEVAARAVVPGAGISAVVVA
                     PVADWSSSNIAMTLLIFFLVILAWAPMCAVFLSRDVVDPLDDITRTVIQVTEVGKLDE
                     VAVIPVVQNDEVGMLALRFNALIDTLRDLGKAASSLARGDLEIRLKGEGDVATAFRSM
                     IDSLGRIVRQIHETSVLLAAAATEIYAASQEQEAAAASQSTAMVEIRQTMESLFEAAA
                     HVSESVRGVLSNAERTLETTDRMVIRIGDLTTHANRIGEILETIRDISDRSDLLALNG
                     ALEASRAGEAGRGFGLVAAEMRRLAERVMSSVHDVRSLVGDIRASGSSTMMATEDSKK
                     LAASTTDAARQITLVTQQQRSGTEQVLQSVREIAETLTQSVAAMAQTRASAEQLKSQA
                     DKLSSLVLAFRIENPPT"
BASE COUNT          216 a          557 c          581 g          249 t
ORIGIN      
        1 ctgggccgcg atctcgggcg gctgggccgc gagctggtcg agcgcggcgc gcaggccgtt
       61 ctcggcggcg ggctcgggag cgtcgtcgcg cagcgcgagc ttgatcgcct cgagcgcgtc
      121 gccgagcacg cgcaccgcgg agcccagggt gcgcggatcg agcaggcgct cctcgcaggc
      181 gtgcgcgacc ttgccgatct ggaccaggcc gagcaggtgc gagtcgccct tgagggtgtg
      241 gagctcgcgg cggacctcgc ccagcgcgtc ggtgttgccg tcggcgaccg cggtcagctt
      301 cgcccggatc gacttgagcc ggtccagcga gatcgcgcgg tagcgcgcca ggagagcttc
      361 gcgccgctgg ctcacgtcgg tgggttctcg atgcggaacg ccaggaccag gctcgacagc
      421 ttgtcggcct ggctcttgag ctgctccgcc gatgcgcggg tctgcgccat cgccgcgacc
      481 gactgggtca gggtctcggc gatctcgcgc acgctctgca ggacctgctc ggtgccgctg
      541 cgctgctgct gggtgaccag cgtgatctgg cgcgccgcat cggtcgtgct cgcggcgagc
      601 ttcttgctgt cctcggtcgc catcatggtc gacgatcccg acgcgcggat gtcgccgacc
      661 agcgagcgca cgtcgtggac cgacgacatc acgcgctcgg cgagccgccg catctcggcc
      721 gcgaccaggc caaagccacg cccggcctcg ccggcccggc tggcctcgag cgcgccgttg
      781 agcgcgagca gatcgctgcg gtcggagatg tcgcgaatgg tctcgaggat ctcaccgatc
      841 cggttcgcgt gggtggtgag atcgccaatc cggatcacca tccgatcggt ggtctcgagt
      901 gtgcgttcgg cgttggacag cacaccgcgg accgactccg acacgtgggc ggcggcctcg
      961 aacagcgact ccatggtctg ccggatctcg accatcgcgg tcgactgcga cgccgccgca
     1021 gcttcttgct cctgcgacgc ggcgtaaatt tcggtcgccg ccgcagcgag cagcaccgag
     1081 gtctcgtgga tctggcgcac gatccggccc aggctgtcga tcatcgagcg gaacgccgtc
     1141 gcgacgtcgc cctcgccctt gagccggatc tcgaggtcgc ccctggccag cgagctggcc
     1201 gccttgccga ggtcgcgcag ggtatcgatc agggcgttga accgcagcgc gagcatcccg
     1261 acctcgtcgt tctggaccac cgggatcacg gcgacctcgt cgagcttgcc gacctcggtg
     1321 acctggatga ccgtgcgggt gatgtcgtcg agcgggtcga cgacgtcgcg cgacaggaac
     1381 accgcgcaca tcggcgccca cgcgaggatc acgaggaaga atatgagcag cgtcatcgcg
     1441 atgttgctcg atgaccagtc ggcgaccggc gcgaccacca ccgcggagat ccccgcgccg
     1501 gggaccacgg cgcgcgccgc gacctcggcg tcgcgcgggc tcgtgatggc gccgtcgccc
     1561 gccgtggttg cgtgcagcca gtcctggagc gggggcgtca ggg
//
LOCUS       DASVOR010001222         2531 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_129166,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001222 DASVOR010000000
VERSION     DASVOR010001222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129166"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..780)
                     /locus_tag="VF516_23190"
     CDS             complement(64..780)
                     /locus_tag="VF516_23190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110664.1"
                     /translation="MTVDAAATIESTPTGVRVVVADDDVVIYRRMAANLPCAGYRVVT
                     AHDGARALAQAEETPPDLAIVDREMPRTHVRAGRAHIAMTGHNGEVSHAKAFGAGPRQ
                     GGADRRMAYGTEAIARFEDAAVKPMVAPPRVQPGAEVGARGERVLAGPWCHDRDRLQD
                     RPLGAVRPTRVERVRHNLAGNAARYCNQGGSIVVASRLERRRTCRDLVTKSGSVPASS
                     CGCPAAPDAFGGSAGRRGLG"
     gene            816..1532
                     /locus_tag="VF516_23195"
     CDS             816..1532
                     /locus_tag="VF516_23195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110665.1"
                     /translation="MTDPHIEASRFADHVDETGAPCARHPAIAPASLYQLAMVGSRAP
                     AFHHDCASKLQGLVMALDELTELTENGDPQLIRAVETALEASRELNALLNVNRTLTRP
                     AARTAIALDELIVRAAERVGVALRGGSPAGVMVNGGVTPMIHALSLAIDVAAGTGRGR
                     TLAITTTPGTTPGTTPGTTPAATPVAGDVELALQSSPLQAPTAGEAIAIAGFVIARDG
                     GGRLWCTSSGGQMVIRLPVG"
     gene            complement(1568..>2531)
                     /locus_tag="VF516_23200"
     CDS             complement(1568..>2531)
                     /locus_tag="VF516_23200"
                     /inference="COORDINATES: protein motif:HMM:NF023746.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8110666.1"
                     /translation="AVDTLIVIPNNRLLALVGTQTSMVEAFRKADSVLLNAVQGISDL
                     MTVPGLINVDFADVRTIMSGMGRALMGTGIGTGKRRATEAAETAISSPLLEDVSIDGA
                     TGILINITGGPDLTLHEVNEASSLIQQAAHEDANIIFGSVIDPNLSEEVRITVIATGF
                     NHSAKTQAAVPHAHTTDRHPAPMVRPRTATGSPQIPLPYASSSQITKEYPVLASPKRP
                     PAQIIEEPEINIDWDKPDTGELTAVEQALSELAEPVPDPEPARMASGSGPAPVDGTPL
                     VQPDRKRAQSRPSVNMKSVLSGEIDTEAELDVPTFIRRHSATQQ"
BASE COUNT          331 a          885 c          917 g          398 t
ORIGIN      
        1 acccgtccga ggtgagtctt cggagtatgc gcgacccgca ccgtcatcga ccttcggtgg
       61 accttacccc aaccctcgcc gcccggccga gccgccaaac gcgtcaggcg cggccgggca
      121 gccgcacgat gaagcgggga ccgatccaga tttagtcacg agatctcgac acgttcgtcg
      181 tcgttccagc cgtgaggcga ccacgatcga tccgccctgg ttgcaatagc gcgctgcgtt
      241 gccggcgagg ttgtggcgca cgcgctcgac ccgcgtgggt cgaaccgcgc caagaggtcg
      301 gtcttgcagc cgatcgcgat cgtgacacca cggcccagcg aggacgcgct cacctcgagc
      361 accgacttca gcacctggtt ggacccgggg cggggcgacc atcggcttga ccgcggcgtc
      421 ctcgaagcgc gcgatggcct cggtgccgta cgccattctc cgatccgcgc ctccttggcg
      481 gggtccggcc ccgaacgcct tcgcgtggct gacctcaccg ttgtggccgg tcatcgcgat
      541 gtgtgcacgg ccggcccgaa catgggtccg gggcatctcc cgatcgacga tcgcgagatc
      601 gggcggcgtc tcctcggcct gggccagcgc ccgcgcgccg tcgtgcgcgg tcaccacccg
      661 gtatcccgcg cagggcaggt tggccgccat ccggcggtag atcaccacat cgtcgtcggc
      721 caccaccacg cgcacgccgg tcggtgtgga ttcgatcgtc gcagccgcat cgaccgtcat
      781 cgagctggta ctatgcggga gcgagcgtga tggcggtgac cgatccccat atcgaagcgt
      841 cgcggttcgc cgatcacgtc gacgagaccg gcgcgccgtg tgcgcgccat ccggcgatcg
      901 cacccgccag cctgtaccag ctcgcgatgg ttggctcgcg cgcgccggcg ttccaccacg
      961 actgcgcgtc caagctgcag ggcctggtga tggcgctcga cgagctcacc gagctgaccg
     1021 agaacggcga cccgcagctg atccgcgccg tcgagaccgc gctcgaggcg tcgcgcgagc
     1081 tcaacgcgct gctcaacgtc aaccgcacgc tgacccggcc ggcggccagg accgcgatcg
     1141 cgctcgacga gctgatcgtc cgtgcggccg agcgggtcgg cgtggcgctg cgcggcggat
     1201 cgcccgccgg ggtgatggtc aacggcggcg tgacgccgat gatccacgcg ctgtcgctcg
     1261 cgatcgacgt cgcggccggc accggccgcg gccgcacgct cgcgatcacc acaactcctg
     1321 gcacaactcc tggcacaact cctggcacca ccccggcggc gacgccggtc gccggcgacg
     1381 tcgagctcgc gctgcagagc tcgccgctgc aggcgcccac cgcgggcgag gccatcgcga
     1441 tcgccggctt cgtcatcgcg cgcgacggcg gcggccggct gtggtgcacc tcgtccggcg
     1501 gccagatggt gatccggctg ccggtgggct gagcccgggc tgagcctggg ttgagcccgg
     1561 gcggagatca ctgctgcgtc gcgctgtggc ggcggatgaa cgtcgggacg tcgagctccg
     1621 cttcggtgtc gatctcgccg gacaggacgg acttcatgtt cacgctcggc cgcgactggg
     1681 cgcgcttgcg atcgggctgg accagcggcg tgccgtcgac cggcgccggg ccggagccgc
     1741 tcgccatccg ggcgggctcg gggtcgggca ccggctcggc gagctcggac agcgcctgct
     1801 cgaccgcggt cagctcgccg gtgtcgggct tgtcccagtc gatgttgatc tcgggctcct
     1861 cgatgatctg cgccgggggc cgcttcggcg acgccagcac cgggtactcc ttggtgatct
     1921 ggctcgagct cgcgtacggc agcgggatct gcggcgagcc ggtggcggtg cgcggccgga
     1981 ccatcggcgc cgggtggcgg tcggtggtgt gggcgtgcgg cacggcggcc tgggtcttgg
     2041 ccgagtggtt gaacccggtc gcgatcacgg tgatccggac ctcctcgctg aggttggggt
     2101 cgatcaccga gccgaagatg atgttggcgt cctcgtgcgc cgcctgctgg atcagcgacg
     2161 acgcctcgtt gacctcgtgc agcgtgaggt cggggccgcc ggtgatgttg atcaggatgc
     2221 cggtggcgcc gtcgatgctg acgtcctcga gcagcggcga gctgatcgcg gtctcggcgg
     2281 cctcggtggc gcgccgcttg ccggtgccga tcccggtgcc catcagcgcg cggcccatgc
     2341 cgctcatgat cgtgcggacg tcggcgaagt cgacgttgat caggccgggg acggtcatca
     2401 ggtcgctgat gccctgcacg gcgttgagca gcaccgagtc ggccttgcgg aacgcctcga
     2461 ccatcgaggt ctgggtgccg accagggcga gcagccggtt gttggggatc acgatcagcg
     2521 tgtcgaccgc g
//
LOCUS       DASVOR010001223         5881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_13221,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001223 DASVOR010000000
VERSION     DASVOR010001223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5881
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13221"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VF516_23205"
     CDS             <1..1017
                     /locus_tag="VF516_23205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110667.1"
                     /translation="DKSGIAGIFAPGAWIAYLRPGNLRPGRPRGGLRRAGAFGGRSAA
                     GAHRRRHDRSACGTCAARVSASRGVSPSTSPRSAGGLALARRSADHPRACCAGPGTRR
                     DGCPSTGDDMSVTSTYTFLQGFFNGMFIDVNTVSSADQPFSESFNGPILTIHRPSNEI
                     AVDLRTLINTNANIGLITKKLMSAGASLTSEIGVQGVLWPSVPYFIQNIAAHKFTDML
                     IRIHFDFHIETPWYCSDADGTISIYLFAFLNSSRNLVVALDGTWWHYDGGGPFCTGAI
                     SSGLNAAMPGVRKTVSDLLPGLLKQTAGIKFSHLYFLPGDGRKAAGAAIENATSDLTI
                     GLTL"
     gene            1032..1352
                     /locus_tag="VF516_23210"
     CDS             1032..1352
                     /locus_tag="VF516_23210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110668.1"
                     /translation="MSNKELFDAVAGALRQAGYTQIAVHDSANESFITATKGPGGAYF
                     RVVQESKPRVAGRTTAGGPEPQPAPHDAHVVPAVAELHHHLRQNPQTAAAFGIPAEAM
                     KHIS"
     gene            complement(1398..2621)
                     /locus_tag="VF516_23215"
     CDS             complement(1398..2621)
                     /locus_tag="VF516_23215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110669.1"
                     /translation="MKSSRDLEIGPLAPPLSMTALGRAVGMAPPISLTALFRRLSQKT
                     FHQVVTAPAPEALGGSITVTIRGDGTLSLKVHMHDSGAPSYAFRLGIFLRATSGRVIA
                     LYSHGTVRGTDYAVTHPFATGFREFDDTPPDDHFPVLDTAWPEFAEGSLSHQLEYSNS
                     LTDFVTSAILGKVKFIAGAVILGGPATLVLLGNAVLRELTGAQLPENLMLGGLVAAET
                     GYFLVGPGGFIPLFIAGALVTAAAFRGRPMRADEIAAAETVFKGTLDYAKITITEFED
                     ARGRNFASPTLDGGVVIGAGSNFDNYLQDDLTRHTFIHELTHAWQAMHGGLPVLSCDG
                     IERSLDENTLSQDRIHDKYYAYQVDPGKAWSEYGPEQQAEIVADYDFIRNLAPSADLG
                     TRQKYIDENIRMGRP"
     gene            complement(2642..3586)
                     /locus_tag="VF516_23220"
     CDS             complement(2642..3586)
                     /locus_tag="VF516_23220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110670.1"
                     /translation="MPPGPHVRDTIETVNAWIRTIDAPNRAIVKGVAAESGDGSRIGV
                     YGSSQTETGVYGQSESGAGVAAMSTSSTGVFGRSESDCGVEGVSNTGPGVVGRGYNGG
                     RGMAGFASSAEGVYGHSKTRAGVVGESDGFDGVFGISHNPAHAGVSGHNPGGLAGYFE
                     GDVRVTGAMTVDRDVVCSNADCAEDFDVVDPSCAEPGAVMVLTETGALEPCTTAYDRR
                     VAGVISGAGGYRPGIILDRSAPREARKPIALMGKVYCRADAGHGAIAVGDLLTTSPTP
                     GHAMRVSDPARAFGAIVGKALAPLASGQGLIPLLVTRQ"
     gene            3782..5188
                     /locus_tag="VF516_23225"
     CDS             3782..5188
                     /locus_tag="VF516_23225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110671.1"
                     /translation="MDAGVRECARGRAHMDRGTRSEEAELFYESLVGEGADEGAEDFY
                     ESLVGEAADERADPFYESLIGEADIDDEATPTAPRDIGKDAFFEEERIKAHMIVGGNP
                     IDPATGATLNQYDAPLVIHAWKTHRTDLPNPLQDGSIALQLEPGGSIFTGQYTIFRPA
                     GLDIGSTATEEIPVALFGGPGAELNRHGLRAAFDRNGKAVLVTLPGRESSPHFGYGIT
                     QAQLVALFRAVGLLGVPRIRVLAGFSTGYRALNGIINNTKSKRTTAPAGTPTGVNPGT
                     GLDLTGVRKVIYFDAFYRGDEPAPGKNTERALKAIHAETGGSARLVIYEVTGGGTPSP
                     LAATPPAGMPLTHINVKPSIEKFTALTLCRVIDMAIKDGYTDPAGVTKVGGKPVLDLL
                     PKLPTRGTVGSAPGSGTTDVTSWCTAAEARAAAAVGPKLITGLIQPNKLVGWAPPNIA
                     EFQHDAHLFEFSWEHLVP"
     gene            complement(5252..5476)
                     /locus_tag="VF516_23230"
     CDS             complement(5252..5476)
                     /locus_tag="VF516_23230"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEX8110672.1"
                     /translation="MPELALDPAGGTATFHAGSPSFYYARVLQTPTNRWSVFDCLRAP
                     NANPTDCAPGGKLDVQIAERAWTSPIWYLP"
BASE COUNT          936 a         2039 c         1996 g          910 t
ORIGIN      
        1 gacaagagcg gcatcgccgg catcttcgcg cccggcgcct ggattgccta cctccggccg
       61 ggaaatctgc gtcccggccg gccacgtggc ggactccggc gggccggcgc gttcggtggc
      121 aggtcggcgg caggcgctca ccggcgaagg cacgatcgat cggcatgcgg cacctgtgcc
      181 gcgcgtgtgt cggcgtctcg cggcgtgagt ccgagcactt cgccccggtc tgccggcggc
      241 ctcgcgcttg ctcgacgctc cgctgaccat ccgcgcgctt gctgcgccgg cccggggaca
      301 cggagagacg gctgcccatc aacaggagac gacatgtcag tcacgagcac atacacattc
      361 cttcaagggt tcttcaacgg catgttcatc gacgtgaaca cggtatcgag tgcggatcag
      421 ccgttcagcg agtcgttcaa cggcccgatc ctcacgatcc atcgccccag caacgagatc
      481 gccgtcgacc tgcgtaccct gatcaacacc aatgccaaca tcgggctcat cacgaagaag
      541 ctgatgtcgg caggggcgtc gctcaccagc gagatcggcg tgcaaggcgt tctatggccg
      601 tcggtgccgt acttcatcca gaacatcgcc gcgcacaagt tcaccgacat gctgatccgg
      661 atccatttcg actttcacat cgagaccccg tggtactgct cggacgctga cggcaccatt
      721 tcgatttacc tgttcgcgtt cctcaactcg agtcgcaacc tggtggtcgc cctcgacggt
      781 acctggtggc actacgatgg cggcggcccg ttctgcaccg gcgcgatctc cagcggcctg
      841 aacgcggcga tgcccggcgt tcgcaagacc gtgagcgatc tgctgcccgg gctgctcaag
      901 cagacggcgg ggatcaagtt cagccacctc tacttccttc ccggcgatgg caggaaggcg
      961 gcgggcgctg cgatcgagaa tgccacctct gatctgacga tcggactgac tctgtaacaa
     1021 ggggaaacga catgagcaac aaggaattgt tcgatgcagt tgccggcgcg ctccgccagg
     1081 ccggctatac gcagatcgcg gtccacgaca gcgcaaacga atcgttcatc accgcgacga
     1141 agggtcccgg aggtgcgtac ttccgggtcg tccaggaatc caagccgcgc gttgccggac
     1201 ggacgaccgc cggcggcccc gagccacagc cagcgccgca cgacgcccac gtcgtgccgg
     1261 cggtcgcgga gctccaccac cacctccggc agaacccgca gaccgccgcg gccttcggga
     1321 tccccgccga ggccatgaag cacatctcgt agctcccggc cggcatcgcg ccggtcgcgg
     1381 ccatgccagc gcgggcgcta cgggcggccc atccggatgt tctcgtcgat gtacttctgg
     1441 cgcgtaccga ggtcggccga cggggcgaga tttctgatga agtcgtagtc ggcgacgatc
     1501 tcggcctgtt gctccggccc gtactcactc cacgccttac cggggtcgac ctggtaggcg
     1561 tagtacttgt cgtggatgcg gtcctgcgac agcgtgttct cgtcgaggga gcgctcgatg
     1621 ccatcgcagc tcaggaccgg gaggccgccg tgcatggcct gccaggcgtg cgtgagctcg
     1681 tggatgaacg tgtggcgagt gaggtcgtcc tggaggtagt tgtcgaagtt cgagcccgcg
     1741 ccgatcacga cgccgccgtc gagcgtcggg ctcgcgaagt tgcgccctcg cgcgtcctcg
     1801 aattcggtga tggtgatctt cgcgtagtcg agcgtgccct tgaacaccgt ctcggccgcg
     1861 gcgatctcgt cggctcgcat cggccggccg cggaacgccg cggcggtcac cagcgcgccg
     1921 gcgatgaaca gcgggatgaa gccgccggga ccgaccagga agtacccggt ctcggcggcg
     1981 acgagcccgc ccagcatcag gttctcgggc agctgcgcgc cggtcagctc gcgcagcacg
     2041 gcgtttccaa gcagcaccag cgtggccggt ccgcccagga tcaccgcgcc tgcgatgaac
     2101 ttgaccttgc cgaggatcgc cgaggtcacg aagtcggtga ggctgttcga gtactcgagc
     2161 tggtggctca gcgaaccctc ggcgaactcg ggccacgcgg tgtcgagcac cgggaagtga
     2221 tcgtcgggcg gggtgtcgtc gaactcgcga aaccccgttg cgaacgggtg ggtgaccgcg
     2281 tagtcggtcc cgcgcacggt gccgtggctg tacagcgcga tgacccgtcc ggacgtggca
     2341 cggagaaata tgccgaggcg aaacgcgtag gacggcgcgc cgctgtcgtg catgtggacc
     2401 ttcaacgaca gcgttccatc tccgcggatc gtgaccgtga tgctgccccc cagagcctcc
     2461 ggcgcaggcg ccgtgacgac ctggtgaaac gtcttctgcg acagtctgcg gaacagcgca
     2521 gtcagcgata tgggcggagc catgccgacg gcgcgcccga gcgccgtcat gctcagcggc
     2581 ggggccagcg gcccgatctc gagatctcgc gagctcttca tggaaatccc tgggcgagca
     2641 gctattgccg ggtgacgagc agcgggatca gcccctgccc ggaggcgagc ggggcgagcg
     2701 ccttgccgac gatggcgccg aacgcccgcg ctggatcgga cacccgcatc gcgtgacccg
     2761 gggtcggcga ggtcgtcagc aggtcgccga ccgcgatggc gccgtgaccg gcgtcggcgc
     2821 ggcagtacac cttgcccatc agagcgatcg gcttgcgggc ctcgcgcggg gcgctgcggt
     2881 cgaggatgat ccccggccgg tagccgccgg cacccgagat cacgcccgcc acccgccggt
     2941 cgtatgccgt ggtgcagggc tccagcgcgc cggtctcggt cagcaccatc accgcgccgg
     3001 gttccgcgca cgacggatcc acgacgtcga agtcctcggc gcagtcggcg ttggagcaga
     3061 ccacgtccct gtcgaccgtc atggcgccgg tcacgcgcac atcgccttcg aagtacccgg
     3121 ccagaccgcc cgggttgtga cccgagacac ccgcgtgcgc ggggttgtgg ctgatgccga
     3181 acacaccatc gaagccatcg ctctcgccga ccacccccgc gcgcgtcttg ctgtgaccgt
     3241 agacgccctc ggcgctgctg gcaaagcctg ccatcccgcg gccgccgttg tagccgcgtc
     3301 ccaccacgcc gggcccggtg ttgctcacgc cctcgacgcc gcagtcggac tcgctgcgcc
     3361 cgaacacgcc ggtcgacgat gtgctcatcg cggctacccc cgcgccactc tcgctttgcc
     3421 cgtacacacc ggtctcggtc tggctcgagc cgtacacgcc gatccgcgat ccgtcgccgc
     3481 tctccgcggc gacccccttc acgatcgcgc ggttgggcgc gtcgatcgtg cggatccacg
     3541 cgttgactgt ctcgatggtg tctctgacat gggggcctgg cggcatctgg tttctctctg
     3601 gaattgcgct cgaccggggc aatgactcgt tgcgggcgcg aggcggtccg ggttcacccg
     3661 tgcacgcctc cagaagacgt cgggacccgc catttcttct ggacaatccg gctctcgatg
     3721 gaagggctat ggccgtatgc tcgagctcac tcccgcgtcc tcatgatatc gcggttgata
     3781 ggtggacgcg ggcgtgagag agtgtgctcg cggcagggcg cacatggatc gcgggacccg
     3841 gagcgaagag gcggagctgt tctacgagtc gctcgtcggc gaaggggccg acgaaggggc
     3901 ggaagatttc tacgaatcgc tcgtcggcga agcggccgac gaacgggcgg atcccttcta
     3961 cgagtcgctc atcggtgaag cggacatcga cgacgaggcg acgccgaccg cgccgcgcga
     4021 catcggcaag gatgcatttt tcgaggagga acgcatcaaa gcgcacatga tcgtcggcgg
     4081 caacccgatc gatccggcca cgggcgccac gctcaaccag tacgacgcgc cgctcgtgat
     4141 ccatgcctgg aagacccacc gcacggatct gccgaacccg ctgcaggacg gctcgatcgc
     4201 gctgcagctc gagccgggcg gcagcatctt caccggccag tacacgatct tcaggcccgc
     4261 gggcctcgac atcggcagca cggccacgga ggagattccg gtcgcgctgt tcggcggtcc
     4321 cggcgccgag ttgaatcgcc acgggctgcg cgcggcgttc gatcgcaacg gcaaggcggt
     4381 gctggtcacg ctgcccgggc gcgagagcag cccgcacttc gggtatggca tcacccaggc
     4441 gcagctcgtc gcgctgttcc gggccgtggg cctcctcggc gttcctcgca tccgcgtgct
     4501 cgccggtttc tcgaccggat atcgcgcgct caacgggatc atcaacaaca ccaagtcgaa
     4561 gcgtacgacg gccccggcgg gcacgccgac cggcgtcaat cccgggaccg gcctcgacct
     4621 gaccggggtc cgcaaggtca tctacttcga tgcgttctat cgcggcgacg agcccgcacc
     4681 cggcaagaac accgagcgcg cgctgaaggc catccacgca gagaccggcg gcagcgcgcg
     4741 gctcgtgatc tacgaggtca ccggcggcgg caccccgtca ccgctcgccg ccacgccgcc
     4801 cgcgggcatg ccgctcacgc acatcaacgt caaaccttcg atcgagaagt tcaccgcgct
     4861 gacgctctgc cgcgtgatcg acatggcgat caaggacgga tacaccgacc cggcgggggt
     4921 gaccaaggtt ggcgggaagc cggtgctgga tctgctgccg aagctaccga cgcgcgggac
     4981 cgtgggctcg gcccccggat cgggcacgac ggacgtcacg agctggtgta ccgccgccga
     5041 ggccagggcc gcggcggcgg tgggccccaa gctcatcacc gggctcatcc agccgaacaa
     5101 gctcgtgggc tgggcgcccc ccaacatcgc cgagttccag catgacgccc acctgttcga
     5161 gttctcgtgg gagcacctcg tgccctgaat ccgtgcgcca cgagatgccc gcgccgcgga
     5221 tcgccgccgg cggcgcgggg ccgggccggg gctacggcag gtaccagatc ggactggtcc
     5281 aggcgcgctc ggcgatctgg acgtcgagct tgccgccggg cgcgcagtcg gtcgggttgg
     5341 cgttcggcgc gcgcaggcag tcgaacaccg accagcggtt ggtcggggtc tgcagcacgc
     5401 gggcgtagta gaagctcggc gagccggcgt ggaacgtggc ggtgccgccg gcggggtcga
     5461 gcgcaagctc tggcacgcgg tcgcgacgcc gccggccggc gcggtccgcc gcacgacctg
     5521 ctcctgcacg cggtcatagg cggcgcgcag ctcggcgtcg ccgggctcgc gggtcgcgcc
     5581 gcccaggctc tcggcgagca gcagcacctt ctggaccacg cgctgctcga tgatcgggtc
     5641 gccctgatcg agctccatcc gcacgccctc aaaaacgcca ccgcgtccat ctggaacggc
     5701 tgccgatgct gcgtcgaggc gcgatcgcca gacgtcaacg cgagccgccg ggggcgaccg
     5761 cgagcaggat ggggtcggac gccgcgaccg tgatttggat tggccgggtc ttgccccaca
     5821 catacctcgc gcggggttcg cgcaggacgg gtggtgattc ggcgggcgcg gctacgacac
     5881 c
//
LOCUS       DASVOR010001224         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_70761,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001224 DASVOR010000000
VERSION     DASVOR010001224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70761"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VF516_23235"
     CDS             <1..455
                     /locus_tag="VF516_23235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110673.1"
                     /translation="VAALGAALGAALAAAAGCSLVPDVGPRLAGTCDDSDGHPGKPVS
                     FSNDIHPLISRGAGGCFCHLPTPNGDGVGTQISGLNLSSLSTLRMGGLHSGTRIIVAG
                     EPCSSILYQKLSDAPPFGSRMPLSGPPYWTADELQLLHDWIAEGANDN"
     gene            577..1311
                     /locus_tag="VF516_23240"
     CDS             577..1311
                     /locus_tag="VF516_23240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110674.1"
                     /translation="MAEKDVSKPAEPPKPVHIGGESLLDRLLPHMKQIIIALVVVSAV
                     LVVIFTIRYFKERGQIADTRKLDQILEVAREPIRNKDEKPDPQKPSFGDPKERGGAVL
                     AEIAKQGTDVAGHAFRAGQLMDAGKVDDAIAEYRQGVGDKTIEGVLCREGLGLALEAK
                     AMTEKDTAARQKGLEEALAAFTAMQPDEAGLRRAYALYHQGRIQQLLGKRAEAKALFE
                     KAKAAIKSDHDLADLVEKRLAALGAS"
     gene            1320..2498
                     /locus_tag="VF516_23245"
     CDS             1320..2498
                     /locus_tag="VF516_23245"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8110675.1"
                     /translation="MKHFAKLALAGAAMVALGVSLGGCEPATWKRADALRAGPPEPLG
                     EDRLSLRWKFVTADRLTEVEPQEFAAAVVYADTVYIGSASGWFFALRSSNGAVRWRKR
                     LVAVASAPIVVAGVLYIGGADGTLLAVDAQTGVEKWRYQSRGPIEQSPVTAGDLIVFS
                     NEADQVAAVDAITGKFKWQYKAETPEEYTLRGHAGVTVDNDLIFTGFSNGTLAALRKE
                     TGTVAWSTSLKAEADRFMDVDATPVVIGDQLYASSSSGGVYALDKATGLVRWRVPFWD
                     AGLPSSTGNVGGIATDGKTLYVSVADLGVYAIDLAGNVLWRVGAKDGGEPAQPVVWNE
                     LLLYSLASDGLFLADRRTGATVEYFDPGAGVSSPPTATADGRLFVMTNRGILYAFDLE
                     "
     gene            complement(2511..>2725)
                     /locus_tag="VF516_23250"
     CDS             complement(2511..>2725)
                     /locus_tag="VF516_23250"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEX8110676.1"
                     /translation="DMSLVRDVDKHQLKQRLIRSVTQLCREQGTQVIGEGVETEAEAQ
                     VLYDLGCDLLQGYVIARPGPPFVDPL"
BASE COUNT          414 a          987 c          951 g          373 t
ORIGIN      
        1 tcgttgccgc gctcggtgcc gcgctcggcg ccgcgctggc cgcggccgcc ggatgctcgc
       61 tggtgcccga cgtcggtccg cggctggccg ggacctgcga cgactccgac ggtcaccccg
      121 gcaagccggt gtcgttctcg aacgacatcc acccgctgat ctcccgcggg gccggcggct
      181 gcttctgcca cctgccgacg cccaacggcg acggggtcgg cacccagatc agcggcctca
      241 acctgagctc gctgtcgacc ctgcggatgg gcgggctcca ctccgggacg cggatcatcg
      301 tcgccggcga accgtgctcc agcatcctgt accagaagct gtcggacgcc ccgccgttcg
      361 ggtcgcggat gccgctcagc ggcccgccgt actggaccgc cgacgagctc cagctgctcc
      421 acgactggat cgccgagggc gcgaacgaca actgagccgg gggccgatcg cccggcggtc
      481 cggccacctc cccaggcaac tccccgggcc gtccccgggt ccaagggtgc gaattgacgc
      541 gccaccgacc ccggtgctac atccccccga cccgccatgg ccgagaagga cgtctccaaa
      601 cctgcagagc ccccgaagcc ggtgcacatc ggaggcgagt cgctgctcga tcgcctgctg
      661 ccgcacatga aacagatcat catcgccctc gtcgtggtgt cggcggtgct ggtggtgatc
      721 ttcacgatcc ggtacttcaa ggagcgcggc cagatcgcgg acacccgcaa gctcgaccag
      781 atcctcgagg tcgcccgcga gccgatccgg aacaaggacg agaagccgga tccccagaag
      841 ccgagcttcg gcgaccccaa ggagcggggc ggggcggtgc tcgccgagat cgccaagcag
      901 ggcaccgacg tcgccggcca cgcgttccgc gccggccagc tgatggacgc cggcaaggtc
      961 gacgacgcga tcgccgagta ccgccagggc gtcggcgaca agacgatcga gggcgtgctg
     1021 tgccgcgagg gcctggggct cgcgctcgag gccaaggcga tgaccgagaa ggacaccgcc
     1081 gcgcgccaga agggcctcga ggaggcgctc gccgcgttca ccgcgatgca gcccgacgag
     1141 gccggcctgc gccgcgccta cgcgctgtac caccagggcc ggatccagca gctgctcggc
     1201 aagcgcgccg aggccaaggc gctgttcgag aaggccaagg ccgcgatcaa gagcgaccac
     1261 gacctcgccg acctggtgga aaagcgcctg gccgcgctcg gggcttcatg aagcaggtca
     1321 tgaaacactt cgccaagctc gcccttgccg gcgcagcgat ggtcgccctc ggggtcagcc
     1381 tcggcggctg cgagcccgcg acgtggaaac gggccgacgc cctgcgcgcc ggtccgcccg
     1441 agccgctcgg cgaggaccgg ctgtcgctgc gctggaagtt cgtgactgcc gaccggctga
     1501 ccgaggtcga gccccaggag ttcgcggccg ccgtggtcta cgccgacacc gtgtacatcg
     1561 gctcggcgag cggctggttc tttgcgctca ggtcgtccaa cggcgcggtg cgctggcgca
     1621 agcggctggt cgcggtcgcc agcgcgccga tcgtggtcgc cggcgtgctg tacatcggcg
     1681 gcgcggacgg cacgctgctc gcggtcgacg cccagaccgg cgtcgagaag tggcgctacc
     1741 agagccgcgg cccgatcgag cagtcgccgg tcacggccgg cgatctgatc gtgttctcca
     1801 acgaggccga ccaggtcgcc gcggtcgacg cgatcaccgg caagttcaag tggcagtaca
     1861 aggccgagac tcccgaggag tacacgctgc gcggccacgc cggcgtcacg gtcgacaacg
     1921 atctgatctt caccgggttc tccaacggca cgctggccgc gctgcgcaag gagaccggca
     1981 ccgtggcgtg gtcgacctcg ctcaaggccg aggccgatcg cttcatggac gtcgacgcga
     2041 ccccggtcgt gatcggcgac cagctctacg cgtcgtcgtc gtcgggcggg gtctacgcgc
     2101 tcgacaaggc caccggcctg gtccgctggc gggtcccgtt ctgggacgcc gggctgccga
     2161 gctcgaccgg caacgtcggc gggatcgcga ccgatggcaa gaccctgtac gtcagcgtcg
     2221 ccgacctcgg ggtctacgcg atcgatctcg ccggcaacgt gctgtggcgg gtcggcgcca
     2281 aggacggcgg cgagccggcg cagcccgtgg tgtggaacga gctgctgctg tactcgctcg
     2341 cgagcgacgg cctgttcctc gccgatcgcc ggaccggcgc gaccgtcgag tacttcgacc
     2401 ccggcgccgg cgtgtcatcg ccgccgaccg ccaccgccga cggccggctg ttcgtgatga
     2461 ccaaccgcgg catcctgtac gcgttcgacc tcgagtaggc cggcacgtac tcagagcgga
     2521 tcgacgaacg gcgggccggg ccgggcgatc acgtagcctt gcagcaggtc gcagccgagg
     2581 tcgtacagca cctgggcctc ggcctcggtc tcgacgccct cgccgatcac ctgcgtgccc
     2641 tgctcgcggc acagctgggt gaccgagcgg atcagccgct gcttgagctg gtgcttgtcg
     2701 acgtcgcgca ccagcgacat gtcga
//
LOCUS       DASVOR010001225         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_420921,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001225 DASVOR010000000
VERSION     DASVOR010001225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_420921"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2019
                     /locus_tag="VF516_23255"
     CDS             <1..2019
                     /locus_tag="VF516_23255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEX8110677.1"
                     /translation="QLAGMRGLMAKPSGEIIETPITTNFREGLSVLQYFISTHGARKG
                     LADTALKTANSGYLTRRLVDVSQDTIISEFDCGTRQGVEMMPLIEGGEIIERLGDRIL
                     GRVVLEDIVDPYTGEVLCPANSEITEDHVKIIDAAGIDRVKIRSVLACETRRGICSLC
                     YGRDLARGHMVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGVGQIRTEQSALEARNEG
                     TVKLNSVQLVKKKDHWVVMNRQGELVLVDDIGRERERYGLQYGARLMIADGTRVKAGQ
                     LLAEWDPFSMPIVTEVAGYVKYGDIIDGVTMQESLDEITGLSRKTVIESKDADSRPRI
                     TLKDADGNTVKLPASGTQEGEARYFLPVGANIFVNDGDYVEAGDVIARISRETTKTKD
                     ITGGLPRVAELFEARKPKDHAVICEIDGVVHFGKDTKGKRKVLVVPEVGDPAEYLIPK
                     GKHLSVREGDRVRAGEALMDGPANPHDILKVLGERALAKYLVDEIQEVYRLQGVRIND
                     KHIEAIVRQMLRRVTVTDPGDTNFLLDEQVEKHIFEEENERVIVAGGQPAKGDALLLG
                     ITKASLSTESFISASSFQETTKVLTEASINGRVDYLRGLKENVIMGRLIPAGTGLGAY
                     KRLTVDVQTDDEGTGYAGSASSSAVGGSAIAAIESEDGHEPAAADAVE"
     gene            2237..2602
                     /locus_tag="VF516_23260"
     CDS             2237..2602
                     /locus_tag="VF516_23260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110678.1"
                     /translation="MATTLSFDKFWAWLAGHANCIVRAGTPEVVLLDHDDFHWTLITE
                     DDHTLVVQLARAKDLVGELLVFPAEIAYVQVEPSETEGEWLFECVVESEKAREVAYHF
                     VMAHEYEDGEHRREEKWTH"
     gene            2708..>3289
                     /locus_tag="VF516_23265"
     CDS             2708..>3289
                     /locus_tag="VF516_23265"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110679.1"
                     /translation="MVDESDIEPDPRIGSVVDGRYKIIEAMAAGSMGVVYKAERVPVG
                     KLVAIKFLHAPFANDSEFLARFERETRVMSKLNHPNCISVVDFGVWDGAPYLVMEYVS
                     GTTLRALIDNGPMPPRRALALIRQIAAGLAHAHAQGVVHRDVKPANIMVSEEIGTGDH
                     VRILDFGLARLRGAVGRDATQSNVVVGTPNYMAP"
BASE COUNT          561 a         1114 c         1150 g          464 t
ORIGIN      
        1 cagctcgccg gcatgcgcgg cctgatggcc aagccgtcgg gcgagatcat cgagaccccg
       61 atcacgacca acttccgtga ggggctgtcg gtgctgcagt acttcatctc gacccacggc
      121 gcgcgcaagg gcctggccga caccgcgctc aagaccgcga actcgggcta cctgacccgc
      181 cgcctggtcg acgtgtcgca ggacacgatc atcagcgagt tcgactgcgg cacccgccag
      241 ggcgtcgaga tgatgccgct gatcgagggc ggcgagatca tcgagcggct cggcgaccgc
      301 atcctcggcc gcgtcgtgct cgaggacatc gtcgacccgt acaccggcga ggtgctgtgc
      361 ccggcgaact cggagatcac cgaggaccac gtcaagatca tcgacgccgc cggcatcgac
      421 cgggtcaaga tccgctcggt gctcgcgtgc gagacccggc gcgggatctg ctcgctgtgc
      481 tacggccgcg acctggcgcg cggtcacatg gtcaacatcg gcgaggcggt cggcgtgatc
      541 gcggcgcagt cgatcggcga gcccggcacc cagctgacga tgcgaacgtt ccacatcggc
      601 ggcgtcggcc agatccgcac cgagcagtcg gcgctcgagg cccgcaacga gggcacggtc
      661 aagctcaact cggtccagct ggtcaagaag aaggaccact gggtcgtgat gaaccgccag
      721 ggcgagctcg tcctggtcga cgacatcggc cgcgagcgcg agcgctacgg cctgcagtac
      781 ggcgcgcggc tgatgatcgc cgacggcacg cgggtcaagg cggggcagtt gctcgccgag
      841 tgggatccgt tctcgatgcc gatcgtcacc gaggtcgccg gctacgtgaa gtacggcgac
      901 atcatcgacg gcgtcacgat gcaggagtcg ctcgacgaga tcaccggcct gtcgcgcaag
      961 accgtcatcg agtccaagga cgccgacagc cgtccgcgga tcacgctgaa ggatgccgac
     1021 ggcaacacgg tgaagctgcc ggcctccggc acgcaggagg gcgaggcgcg ctacttcctg
     1081 ccggtcggcg ccaacatctt cgtcaacgac ggcgactacg tcgaggccgg agacgtgatc
     1141 gcgaggatct cgcgcgagac caccaagacc aaggacatca ccggcggcct gccgcgcgtc
     1201 gccgagctgt tcgaggcccg caagcccaag gaccacgcgg tgatctgcga gatcgacggc
     1261 gtggtgcact tcggcaagga caccaagggc aagcgcaagg tcctggtcgt gcccgaggtc
     1321 ggcgatcccg ccgagtacct gatccccaag ggcaagcacc tgtcggtccg cgagggcgac
     1381 cgggtccgtg ccggcgaggc gctgatggac ggtccggcca acccgcacga catcctcaag
     1441 gtgctcggcg agcgcgcgct ggccaagtac ctggtcgacg agatccagga ggtctaccgg
     1501 ctgcagggcg tgcggatcaa cgacaagcac atcgaggcga tcgtccgcca gatgctgcgc
     1561 cgcgtgacgg tgaccgatcc gggcgacacc aacttcctgc tcgacgagca ggtcgagaag
     1621 cacatcttcg aggaggagaa cgagcgggtg atcgtcgccg gcggccagcc ggccaagggc
     1681 gacgcgctgc tgctcggcat cacgaaggcg tcgctgtcga ccgagtcgtt catctcggcg
     1741 tcgtcgttcc aggagaccac caaggtgctg accgaggcct cgatcaacgg ccgggtcgac
     1801 tacctgcgcg gtctcaagga gaacgtgatc atgggccgct tgatcccggc gggcaccggc
     1861 ctgggcgcct acaagcgcct cacggtcgac gtccagaccg acgacgaggg caccggctac
     1921 gccggcagcg cgagcagcag cgcggtcggt ggcagcgcca tcgcggcgat cgagagcgag
     1981 gacgggcacg agccggcggc cgccgacgcc gtcgagtgat cggcgccgcc cgggtggtcc
     2041 ggccagccat ctgaccgaga tttcgcgcgg ttagccaccg ggctccggct cgtccttgcc
     2101 cgctgccggg gtggcgcggt cggcggcacc tcgccagcgg gcccgggcca cccggacagc
     2161 ctgctaccgc ttgcactccg tgggggcgtg gctatcctcc gcggcgatgg cttcgacggg
     2221 caagagactg gcagccgtgg cgaccacgct cagcttcgac aagttctggg cctggctcgc
     2281 cggtcacgcg aactgcatcg ttcgcgcagg caccccggag gtcgtcttgc tggatcacga
     2341 cgacttccac tggacgctga tcaccgagga cgatcacacg ctggtggtcc agctcgcgcg
     2401 cgccaaggac ctggttggtg agttactcgt gttccccgcc gagatcgcct acgtccaggt
     2461 cgagccgtcg gagaccgagg gcgagtggct gttcgagtgc gtggtcgaga gcgagaaggc
     2521 gcgcgaggtc gcgtatcact tcgtgatggc ccacgagtac gaggacggcg agcaccggcg
     2581 cgaggagaag tggacgcact gagctagcgt ccgccgccgg cgacaccgta cctcccgggt
     2641 acctctgggt accctcctgg caggaggcga gccggcgcga tcgccgtgct aggctccggg
     2701 ctggctcatg gtggacgagt cagacatcga gccggatccg cggatcggca gcgtcgtcga
     2761 cgggcgctac aagatcatcg aggcgatggc ggccggctcg atgggcgtgg tgtacaaggc
     2821 cgagcgggtc cccgtcggca agctggtcgc gatcaagttc ctccacgcgc cattcgccaa
     2881 cgactcggag ttcctcgcac ggttcgagcg cgagacccgg gtgatgagca agctcaacca
     2941 ccccaactgc atctcggtcg tcgacttcgg ggtgtgggac ggcgcgccgt acctggtcat
     3001 ggagtacgtg tcgggcacga cgctgcgcgc gctgatcgac aacggcccga tgccgccgcg
     3061 ccgcgcgctc gcgctgatcc gccagatcgc cgccgggctc gcccacgccc acgcccaggg
     3121 cgttgtccac cgcgacgtca agccggcgaa catcatggtc agcgaggaga tcgggaccgg
     3181 cgaccacgtc cgcatcctcg acttcgggct cgcgcggctg cgcggcgcgg tcggccgcga
     3241 tgccacgcag tccaacgtcg tcgtcggcac gcccaattac atggcgccg
//
LOCUS       DASVOR010001226         2569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_158315,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001226 DASVOR010000000
VERSION     DASVOR010001226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158315"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VF516_23270"
     CDS             complement(<1..381)
                     /locus_tag="VF516_23270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110680.1"
                     /translation="MLQGLVACRRHPPARESLDAERSRRGIPGAAIAFVARGAPDELH
                     AIGELDADSGVEVASLSKPVFAFAVISQAVRGDRPLGFVAPPGAGWQYSGEGYVLLQR
                     ALEARGTALDELVGATVLSPLGMAR"
     gene            complement(429..836)
                     /locus_tag="VF516_23275"
     CDS             complement(429..836)
                     /locus_tag="VF516_23275"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8110681.1"
                     /translation="MTTLRKHTGGCHCGAVRFEVEIDAATGTMCNCTICAKTAWVSGR
                     VQPAAFHLRSGEASLSSYEWGGKIMQRRFCKHCGVQCFGAGHLDVLGGDFVAVNLCTL
                     DGFDLSQIQLSYWDGRHNNWQAGPRPTPWPVSA"
     gene            complement(953..1627)
                     /locus_tag="VF516_23280"
     CDS             complement(953..1627)
                     /locus_tag="VF516_23280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110682.1"
                     /translation="MRTGSTGPAAPLWRVQRGLEELYRVHTGVEIGDFVVGAELRDAL
                     VAARRPREQLLVCEAEGEMALALFIDPRALANLSSHDPARRLGDHNLGDFLLVIEGVS
                     HFIYAIRCARAERPVSQLELELQAEVDKYVTCLLATEPLADVSAALRRRLFDDAAYEP
                     DLDHDERARYRAANDNAQRYAAYLEQAFVVPRRIPEMLAELRRFYRLGLAGKLGSIAR
                     AAGRAA"
     gene            complement(1629..2201)
                     /locus_tag="VF516_23285"
     CDS             complement(1629..2201)
                     /locus_tag="VF516_23285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110683.1"
                     /translation="MDVTLATSVDEFFHEVVTDALSAVDLDASESAGWYLVGLLGEFT
                     TVRLTDEPLGLKLAAAPDSPGERVRTLKEVGDTSLYVAGFFAESLTRSLIDVEYYVGL
                     GQTAYTRLARSLGGRKSIAEVYEELAAKFPRFVDVLMEVRKRVTIAELNASSDIGRLY
                     DIWLRTRDEWVEKKLKQAGLLVDPGSKVIQ"
     gene            2466..>2569
                     /locus_tag="VF516_23290"
     CDS             2466..>2569
                     /locus_tag="VF516_23290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX8110684.1"
                     /translation="MNVRPLQDRILVRRVEEVEKTRGGIIIPDSAKERP"
BASE COUNT          428 a          894 c          877 g          370 t
ORIGIN      
        1 gcgtgccatc ccgagcggcg acagcacggt ggccccgacc agctcgtcga gcgccgtgcc
       61 ccgcgcctcc agcgcgcgct ggagcagcac gtagccttcg ccggagtact gccagcccgc
      121 gccgggcggt gcgacgaacc cgagcggccg atcgccgcgc actgcctggg agatcaccgc
      181 gaacgcgaac accggcttgg acagcgacgc gacctcgact cccgagtcag catcaagctc
      241 gccgatcgca tggagctcgt cgggtgcgcc gcgcgcgacg aaggcgatcg ccgcgccggg
      301 gatcccgcgc cgggagcgct cggcgtcgag cgactcgcgc gccggtggat gacgccggca
      361 cgccaccagc ccctgcagca ccgattggac aaaggctcgc cgcgaccaca tcaccgcttc
      421 gggcgagcct acgccgagac cggccacggc gtggggcgcg gaccggcttg ccagttgtta
      481 tggcggccgt cccagtagct gagctggatc tggctcaggt cgaagccatc gagagtgcac
      541 aggttgaccg ccacgaagtc gccgccgagc acatcgaggt gcccggctcc gaagcactgc
      601 acgccgcagt gcttgcagaa cctgcgctgc atgatcttgc cgccccactc gtacgacgac
      661 aggctggcct cgccggacct caggtggaac gccgcgggct ggacgcgccc gctgacccag
      721 gcggtcttcg cgcagatcgt gcaattgcac atcgtcccgg tcgcggcatc gatctcgacc
      781 tcgaagcgga ccgcgccgca gtggcagccg ccggtatgct tgcggagagt ggtcatcgtc
      841 atgctccttt cagaggaaag tttgtgatgg agcaacccta gcaagctacc ttgacatcct
      901 tatgtcaact ttcggcccgc cgcgaccccc ggccgttgcc gtgcgcgatc gctcaggcgg
      961 cgcggccggc cgcgcgcgcg atcgagccga gcttgccggc gaggccgagc cggtagaacc
     1021 ggcgcagctc ggcgagcatc tcggggatcc tgcgcggcac gacgaacgcc tgctcgaggt
     1081 atgcggcgta gcgctgcgcg ttgtcgttgg cggcgcggta gcgggcacgc tcgtcgtgat
     1141 cgagatcggg ttcgtacgcg gcgtcgtcga acagccgccg gcgcagcgcg gccgagacgt
     1201 cggccaaggg ctcggtggcg agcaggcacg tgacgtactt gtcgacctcg gcctggagct
     1261 cgagctcgag ctggctgacc ggccgctcgg cgcgcgcgca ccggatcgcg tagatgaaat
     1321 ggctcacgcc ctcgatcacg agcaggaagt cgccgaggtt gtggtcgccg agccggcgcg
     1381 ccgggtcgtg ggacgaaagg ttcgcgagcg cgcgcgggtc gatgaacagc gcgagcgcca
     1441 tctcgccctc ggcctcgcag accagcagct gctcgcgcgg ccggcgcgcg gcgaccagcg
     1501 catcgcgcag ctcggcgccg accacgaagt cgccgatctc gacgccggtg tgcacgcggt
     1561 acagctcctc gaggccgcgc tggacccgcc acagcggcgc tgcggggccg gtactgccgg
     1621 tgcgcacctt attgaatgac cttgctgccc gggtcgacca gcaggccggc ctgcttgagc
     1681 ttcttctcga cccactcatc gcgggtgcgg agccagatgt cgtacagccg gccgatgtcg
     1741 ctgctcgcgt tgagctcggc gatcgtcacc cgcttgcgga cctccatcaa cacgtcgacg
     1801 aaccgcggga acttggccgc gagctcctcg tagacctcgg cgatcgactt gcggccgccg
     1861 agcgagcgcg ccagccgggt gtaggcggtc tggcccagcc cgacgtagta ctcgacgtcg
     1921 atcagggacc gcgtcagcga ctcggcgaag aaccccgcga cgtacagcga ggtgtcgccg
     1981 acctccttga gggtgcggac acgctccccc gggctgtccg gagcggcggc gagcttgagg
     2041 ccgagcggtt cgtccgtgag acggaccgtg gtgaactcac cgagcagacc caccaggtac
     2101 caacccgccg attccgaggc atcgaggtcg acagccgaga gggcgtcggt gacgacctcg
     2161 tggaagaact cgtccacgga agtagccagc gtgacatcca tgatccgatc ttcggccagt
     2221 tcgcgccgaa ggcaagatca ccttttatcc cagggtcttg gagcgggagc gaaatcggcc
     2281 atcgcggcgg ggtcgcggcg cctgaaggcc gttccggtcc gagccccagt cgccaggaaa
     2341 atcgaaccag tagccccttg cgcggtgcgg ccagacgcct taccaagccg ctgttagcag
     2401 tcacccgagg tgagtgctaa caaaacgacg gttttgccaa gaaacaccac ggagagagcc
     2461 acgccatgaa cgtccgcccg cttcaggatc gcatcctggt tcgccgcgtc gaggaggtcg
     2521 agaagacccg cggcggcatc atcattcccg actccgccaa ggagcgccc
//
LOCUS       DASVOR010001227         5412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_70832,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001227 DASVOR010000000
VERSION     DASVOR010001227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70832"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..655)
                     /locus_tag="VF516_23295"
     CDS             complement(<1..655)
                     /locus_tag="VF516_23295"
                     /inference="COORDINATES: protein motif:HMM:NF025059.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8110685.1"
                     /translation="MKHDPRTDPEVPPGMSIALLQDLHLLTRDGHLNADARRKLKQIR
                     HLLQLLRPALDDALARAADPLIVDCGAGKSYLGALLYELVLAPAGKGTLLAIEARPEL
                     SAQAQQRAARFGQDRFQVATGAIAHAAPPRRPSVVTALHACDTATDEALALAIERSAD
                     HVAVVPCCQAEVARQLEASRPADPAIAALFAHPLHRRELGSHLTNVVRGLALEAHGYK
                     "
     gene            771..1031
                     /locus_tag="VF516_23300"
     CDS             771..1031
                     /locus_tag="VF516_23300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110686.1"
                     /translation="MDEDEADETAYELSERFFEYDEFGRPLDPDTYRRRQAWKKVREM
                     IAKMTPEEAGEFLVKLGTHNPDGTLTEHYADNGEPSKYRPTD"
     gene            complement(1105..1629)
                     /locus_tag="VF516_23305"
     CDS             complement(1105..1629)
                     /locus_tag="VF516_23305"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8110687.1"
                     /translation="MQTPERADLDLDRTRAYVRSIAMKYVHNEHDAEDVTQDAMLLAY
                     RYRDAFRGDSRYTTWLYRVTATAALMFLRKQRRLSREIPASGTGTDDEATPWLERVAA
                     PTDLRAEVIARADLSVVADAVARLGAKYPAVFWKRYGEGRTETEIAKELGVSVAAVKT
                     RAFRARQAALSSAG"
     gene            1790..3268
                     /locus_tag="VF516_23310"
     CDS             1790..3268
                     /locus_tag="VF516_23310"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8110688.1"
                     /translation="MLNAVPHVLVVDAVSDTVAYAVASLPGMELEAVATPAAALERVV
                     GSAFDAVLLDPVLGDGDDGALALLHRVRTVAPDTVVVIWSEAPTVGFAVRAMRAGALD
                     VLKKTADAAEIRSVVERAISHGALAREVRRLRGEVERARGLGEVIGESALMRQLLAMI
                     DRVAASDATVLIVGESGTGKELLARTIHRIGPRSDGPFVAFDCSALAPTLLEAELFGH
                     EKGAFTGASRARRGLFREAQAGTIFLDEIGDIDASVQNKLLRVLQEREIKPVGSDRPV
                     AIDVRVVAATNKDLKALVARGQFREDLYWRLAVVPIQVPPLRERKEDIRHLAAHILAR
                     RRGAAKSFAGSEARYPTQITAKALARLESYRWPGNIRELENVLSRAAILCDGEIIRSH
                     DLDMLGLDRPHAGAGAGPGSGDDERIELPPIELDRLGDGEGLKDITERAVRAVERAAL
                     AAALRRDRNPAIAARRLGISRASIYTKMKTYSLGPDGSSSAD"
     gene            3310..4017
                     /locus_tag="VF516_23315"
     CDS             3310..4017
                     /locus_tag="VF516_23315"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HEX8110689.1"
                     /translation="MTYELYYWPEIQGRGEFVRLVLEDADCAYVDVGREPGGYERLEA
                     VLAGEHDGVLPFALPVLYAGPIVVAQTVAITSFLGARHGLAPTDERGRLGALTVALTI
                     ADLVTEVHDTHHPITVEERYETQKAAAKQRAAAFRRSRLPKFLGYLEANLERNGEVLV
                     GSAVSYADLAAFQVVEGLSYAFPRAMARIRDDIPRLLALRDRVAQRTRLLHYLTSPRR
                     LPFNEQGIFRHYPELDG"
     gene            4039..4386
                     /locus_tag="VF516_23320"
     CDS             4039..4386
                     /locus_tag="VF516_23320"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8110690.1"
                     /translation="MTEDWRAVARIGDIPRGDVIAVTVDGIEMVLGRDGDRYFATQRR
                     CLHRGGDLAEGIVSRGHLICPQHAWRFSTATGCNAEASEFCLVTYPVRVVGDQIEVDP
                     RPRPGPGAPPPEP"
     gene            4399..>5412
                     /locus_tag="VF516_23325"
     CDS             4399..>5412
                     /locus_tag="VF516_23325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HEX8110691.1"
                     /translation="MSVPTVSLPTVSLIEGDGIGPEITAATLRAVEACGGQLAWERVD
                     AGAGAVARHGDPLPAATLASIQRNQLALKAPLATPSGGGYRSVNVTLRQHFDLYANVR
                     PARTIAGVPARFTGVDLVIVRENTEDLYAGIEHYVDPRRTAAESIAIITRFGSERVIV
                     YAFEYARRHGRRKVTLVHKANILKMSNGLFLDTGREVARRYPDIEFEDMIVDATAMKL
                     VIAPERFDVIVTMNLFGDILSDLAAGLIGGLGVAPAANIGDSGCAMFEAVHGTAPDIA
                     GKGIANPSGLMLSAAMLLDHVEQRGAAQRLRAGVAAALARPETRTGDLGGRANTAQFT
                     DAVI"
BASE COUNT          776 a         1863 c         2043 g          730 t
ORIGIN      
        1 ccttgtagcc gtgagcctcg agggcgaggc cgcggaccac gttggtcagg tgcgagccga
       61 gctcgcggcg atgcagcgga tgggcgaaca gcgcggcgat cgccggatcg gccgggcgcg
      121 acgcctcgag ctggcgcgcg acctcggcct ggcagcacgg gacgaccgcg acgtggtcgg
      181 cggagcgctc gatcgccagc gcgagggcct cgtcggtcgc ggtgtcgcag gcgtgcagcg
      241 cggtgacgac gctcggtcgc cggggcggtg cggcgtgggc gatcgcgccg gtggcgacct
      301 ggaagcgatc ctgaccgaat cgcgcggcgc gctgctgggc ctgcgccgac agctcggggc
      361 gggcctcgat cgcgagcagc gtgcccttgc cggcgggggc cagcacgagc tcgtagagca
      421 gggcgccgag gtagctcttg ccggcgccgc agtcgacgat cagcgggtcg gccgcgcgcg
      481 ccagggcgtc gtcgagcgcc ggccgcagca gctggagcag gtggcggatc tgcttgagct
      541 tgcggcgcgc gtcggcgttg aggtggccat cgcgggtcag gaggtggagg tcctgcagca
      601 gcgcgatcga catccccgga gggacctcgg gatcggtgcg cgggtcgtgc ttcacgccgc
      661 caccttagcg tggacggggc cggcggacag cgcccggcgc gcagcgaggc ggcgccatgc
      721 gatatgggtc cggcttgcat cctcggcgtg ttccgcccca cagtccaggc atggatgagg
      781 acgaggccga cgagaccgcc tacgagctca gcgagaggtt cttcgagtac gacgagttcg
      841 ggagacctct agatcccgac acgtaccgcc ggcgacaggc ttggaagaag gtgagagaga
      901 tgatcgcgaa gatgaccccc gaggaggccg gggagttcct cgtcaagctc ggcacccaca
      961 accccgacgg aacgctgacc gagcattacg cagacaacgg cgagcccagc aagtaccggc
     1021 cgaccgactg aaaccgacgg accaggccga gcgcgcgcgg atcgcacagc ggcgcgatcc
     1081 gccgcggagc gcgagcggcg atgctcagcc ggcgctggac agcgcggcct ggcgagcgcg
     1141 gaacgcgcgg gtcttgaccg cggcgaccga gacgccgagc tccttggcga tctcggtctc
     1201 ggtgcggccc tcgccgtagc gcttccagaa caccgcgggg tacttggcgc cgagccgggc
     1261 gaccgcgtcg gcgacgacgc tgagatcggc gcgggcgatg acctcggcgc gcagatcggt
     1321 cggcgcagcg acccgctcga gccacggcgt cgcctcgtcg tcggtgccgg tgccgctcgc
     1381 ggggatctcg cgcgacagcc ggcgctgctt gcgcaggaac atgagcgcgg cggtcgcggt
     1441 cacgcggtag agccaggtcg tgtagcgcga gtcgccacgg aaggcgtcgc ggtagcggta
     1501 ggcgagcagc atcgcgtcct gcgtcacgtc ctccgcgtcg tgctcgttgt gcacgtactt
     1561 catcgcaatc gacctgacgt aggcgcgggt gcgatcgagg tcgagatccg cgcgctctgg
     1621 tgtctgcatc gaaccctcct ggttcgaacc gatacgcacg cagcgcgcca gccctgtcca
     1681 ggcagttcga caagcagttc aaggtatttg agtgagatgt ggcccccggg attgtccagg
     1741 ctgctggacg ctgtctagac agctggacgg catcgggtac gctgatctcg tgctcaacgc
     1801 ggtgccccat gtgctggtgg tcgatgcggt gtcggacacc gtcgcctacg ccgtggcgtc
     1861 gctgcccggc atggagctgg aggcggtggc gacgccggcg gcggcgctgg agcgcgtggt
     1921 gggctcggcg ttcgacgcgg tgctgctcga ccccgtcctg ggcgacggcg acgacggcgc
     1981 actcgcgctg ctccaccgcg tgcgcacggt cgcaccggac accgtggtgg tgatctggag
     2041 cgaggcgccc accgtcggct tcgcggtgcg cgcgatgcgc gccggcgcgc tcgacgtgct
     2101 caagaagacg gcggacgccg cggagatccg ctcggtggtc gagcgcgcga tcagccacgg
     2161 tgcgctggcg cgcgaggtcc gccggctgcg cggcgaggtc gagcgcgccc gcgggctggg
     2221 cgaggtgatc ggcgagtcgg cgctgatgcg ccagctgctc gcgatgatcg accgggtcgc
     2281 ggcgtcggac gccaccgtgc tgatcgtcgg cgagagcggc accggcaagg agctgctggc
     2341 gcgcacgatc caccggatcg ggccgcgcag cgacgggccg ttcgtggcgt tcgactgctc
     2401 ggcgctggcg ccgaccctgc tggaggccga gctgttcggt cacgagaagg gggcgttcac
     2461 cggggccagc cgggcgcgcc gcgggctgtt ccgcgaggcc caggccggca cgatcttcct
     2521 cgacgagatc ggcgacatcg acgccagcgt ccagaacaag ctgctgcgcg tgctccagga
     2581 gcgcgagatc aagccggtgg gcagcgaccg gccggtcgcg atcgacgtgc gggtggtcgc
     2641 cgcgaccaac aaggacctga aggcgctggt cgcgcgcggc cagttccgcg aggacctgta
     2701 ctggcggctg gcggtcgtgc cgatccaggt cccgccgctg cgcgagcgca aggaggacat
     2761 ccgccacctc gccgcgcaca tcctggcgcg ccggcgcggc gcggccaaga gcttcgccgg
     2821 cagcgaggcg cgctacccga cgcagatcac cgccaaggcg ctggcccggc tcgagtcgta
     2881 ccgctggccg ggcaacatcc gcgagctgga gaacgtgctg tcgcgcgccg cgatcctgtg
     2941 cgacggcgag atcatccgat cgcacgatct cgacatgctc gggctcgacc ggccgcacgc
     3001 cggggccggc gccggcccgg gcagcggcga cgacgagcgc atcgagttac caccgatcga
     3061 gctcgaccgg ctgggcgatg gggaggggct caaggacatc accgagcgcg cggtgcgcgc
     3121 cgtggagcgc gccgcgctgg ccgccgcgct gcgccgggat cgcaacccgg cgatcgccgc
     3181 gcgccggctc gggatcagcc gggcctcgat ctacaccaag atgaagacct acagcctcgg
     3241 gcccgacggc agctcgtcgg cggactgagc ggcccgggcg cgccgcgccg tcgcgggcta
     3301 gccttgggca tgacctacga gctctattac tggcccgaga tccaggggcg cggcgagttc
     3361 gtgcgcctgg tgctcgagga cgccgactgc gcgtacgtcg acgtggggcg cgagccaggc
     3421 ggctacgagc ggctcgaggc ggtgctcgca ggcgagcacg acggcgtcct gccgttcgcg
     3481 ctgccggtgc tgtacgccgg cccgatcgtc gtggcgcaga ccgtcgcgat cacgagcttc
     3541 ctcggggcgc gccacggcct ggcgcccacc gacgagcgcg gccggctcgg cgcgctgacc
     3601 gtggcgctga ccatcgccga cctggtcacc gaggtccacg acacgcatca tccgatcacg
     3661 gtcgaggagc gctacgagac ccagaaggcc gccgccaagc agcgcgcggc ggcgttccgg
     3721 aggtcccggc tcccgaagtt cctcggctac ctcgaggcca acctcgagcg caacggcgag
     3781 gtcctggtcg gctcggcggt gtcctacgcc gacctcgcgg cgttccaggt cgtcgagggc
     3841 ctgtcgtacg cgttcccgcg tgcgatggcg cggatccgcg acgacatccc ccggctgcta
     3901 gcgctgcgcg accgggtggc gcagcggacg cgcctgctgc actacctgac ctcaccgcgc
     3961 cggttgccgt tcaacgagca gggaatcttc cgacactatc cggagctcga tggctgagtc
     4021 gcgctaagct cggcggccat gacggaggac tggcgcgcgg tcgcgcggat cggcgacatc
     4081 ccgcgcggcg acgtgatcgc ggtgacggtc gacggcatcg agatggtgct cggccgcgat
     4141 ggcgatcgct acttcgccac ccagcggcgc tgcctccacc gcggcggcga tctggcggag
     4201 ggcatcgtgt cgcgcggcca cctgatctgc ccgcagcacg cgtggcggtt ctcgactgca
     4261 accggttgca acgccgaagc atccgagttc tgcctggtga cctatccggt gcgcgtggtc
     4321 ggcgaccaga tcgaggtcga tccgcggccg cgaccggggc cgggcgcgcc accgcccgag
     4381 ccgtgagagg agtgaaccgt gagcgtcccg accgtgagct tgccgaccgt gagcttgatc
     4441 gaaggtgatg gcatcggccc cgagatcacc gccgcgacgc tgcgcgcggt cgaggcgtgc
     4501 ggcgggcagc tcgcgtggga gcgggtcgat gccggcgcgg gcgcggtggc ccggcacggc
     4561 gatccgctgc cggccgcgac gctggcgtcg atccagcgca accagctggc gctcaaggcg
     4621 ccgctcgcga ccccgagcgg cggcggctac cgctcggtca acgtgacgct gcgccagcac
     4681 ttcgacctct acgccaacgt gcggccggcc aggacgatcg ccggcgtgcc ggcgcggttc
     4741 accggcgtcg atctggtgat cgtgcgcgag aacaccgagg acctctacgc cggcatcgag
     4801 cactacgtcg acccgcggcg gaccgcggcg gagtcgatcg cgatcatcac ccggttcggc
     4861 agcgagcgcg tgatcgtgta cgcgttcgag tacgcgcggc gccacggccg gcgcaaggtc
     4921 acgctggtcc acaaggcgaa catcctcaag atgtccaacg ggctgttcct cgacaccggg
     4981 cgcgaggtcg ccaggcgcta ccccgacatc gagttcgagg acatgatcgt cgacgccacc
     5041 gcgatgaagc tggtgatcgc gcccgagcgg ttcgacgtga tcgtcacgat gaacctgttc
     5101 ggtgacatcc tgtccgacct cgccgccggg ctgatcggcg ggctcggcgt cgctccggcg
     5161 gccaacatcg gcgacagcgg ctgcgcgatg ttcgaggcgg tgcacggcac cgcgcccgac
     5221 atcgccggca agggcatcgc caacccgagc gggctcatgc tgtcggcggc gatgctgctc
     5281 gatcacgtcg agcagcgcgg ggcggcccag cggctgcgcg cgggcgtcgc ggcggcgctg
     5341 gcccgccccg agacccgcac tggcgatctc ggcggtcgcg cgaacacggc gcagttcacc
     5401 gacgcggtga tc
//
LOCUS       DASVOR010001228         3790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_100845,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001228 DASVOR010000000
VERSION     DASVOR010001228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3790
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100845"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VF516_23330"
     CDS             complement(<1..868)
                     /locus_tag="VF516_23330"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8110692.1"
                     /translation="MTRAARLLAILLVLAIVFPWLAGPYALSVGTLILYFAYTGQAWN
                     VMMGFAGQLSLGHALYVGIGAYAAGGLYYHLGIGPWAGLPVAIAVAALVGVVIGCLAF
                     RFGVSGVYFALLTIAFAEFTRIGFDHMEWTGGPGGMFLPVAERDRIDLLHFRGPPALY
                     YYAMLALTAGALALCARLVDSRAGYYWRAIREDEDAAQALGVHVFRWKLLAVAISAGM
                     TAISGVFFAFYYNNLFPGEIFSAGRSIEIILGSVIGGVGTLFGPILGAAVLTLLSEGV
                     TEALAALGWEVPG"
     gene            complement(865..1734)
                     /locus_tag="VF516_23335"
     CDS             complement(865..1734)
                     /locus_tag="VF516_23335"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8110693.1"
                     /translation="MPFEFVVAGVLAGILTGLVYGLMALGLSVIFGVVRVVNFAHGEL
                     MTVAMYAATLLFAHYELDPFLAVLPVAAAFFVLGYALQAGLINRFITRPEHSQFMLLV
                     AVGLIVINGLLMAFGPDARSVQVDYQLESFELGDIVIDKARLYAAGLAVLTALALIGV
                     FRFTRTGKAIRACADNHFAARVVGLNVKRLYAITFGLGAVCVAVAGCSLTLLFDVTPS
                     VGPQYTLLSFVIVIVGGMGSMLGALLGGVLIGVSEALAGIYISPSAKSMLSFGLLIVV
                     LIVRPQGLFGRKP"
     gene            complement(1736..2977)
                     /locus_tag="VF516_23340"
     CDS             complement(1736..2977)
                     /locus_tag="VF516_23340"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8110694.1"
                     /translation="MASSQGITRRTFQSVLATGLASSLLPLRSARAQAPKLKVGVILP
                     RSGTLGLIGQSCQKGADLAPGVIKEMLGVELELMNADFESNIDTARTRAERLIQDGAH
                     VLVGPFDSGAASAIAQVAEQRGVPLIINIAAAPQITEQGYKFVFRNFQTAGDIVKNGL
                     DLIGELFQTTHAAPTTAVYMHVNDTFGQAMTKAITAMLPSRSQLGFKIVDDIGYDPAA
                     KDLTVEVSRAKAAKADFLLLVSRLNDAILLRREMVKQRWNPMGIISPGSPGMYEEQYV
                     KGLGKLAEYSISNVPWFNPTANLTKIVEKAFVKQNPNDKLVFHGLNVGFTFEAILVAA
                     DAFKRARTTDPKALAEAIRQTHITDKMTLGGPIKFNPKGQVEGIGSACVQNLNQLPTV
                     VLPAAAATAKPVFPRPDYKKT"
     gene            complement(3039..3494)
                     /locus_tag="VF516_23345"
     CDS             complement(3039..3494)
                     /locus_tag="VF516_23345"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8110695.1"
                     /translation="MSEPTDFSAMLNTRLGGWNTAMGLRFSKVTTDECIGEVAIADIH
                     RQPYGIVHGGVHAGIIEAACSTGAAVVALARGQSVVGVENSTSFIAAVRDGTLHVTAT
                     PVTRGRRTQVWQATITNDAGKICSTGRVRLLCLDANTDLAGKPVEPPET"
     gene            3576..>3790
                     /locus_tag="VF516_23350"
     CDS             3576..>3790
                     /locus_tag="VF516_23350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110696.1"
                     /translation="MPGRTRRLVPLIPAPGDPAGRRAQAQRDGYLYLPRLLRDSTLAP
                     LRALVEGALTRRGWLRGATTDPALRLGR"
BASE COUNT          623 a         1303 c         1338 g          526 t
ORIGIN      
        1 cgcccgggac ctcccagccg agcgcggcca gcgcctcggt gacgccttcg gacagcagcg
       61 tgagcaccgc ggcgccgagg atcgggccga acagcgtacc gacgccgccg atcaccgagc
      121 cgaggatgat ctcgatcgag cgcccggcac tgaagatctc cccgggaaac aggttgttgt
      181 aatagaatgc gaagaacacg cccgagatcg ccgtcatccc ggcgctgatc gccaccgcga
      241 ggagcttcca gcgaaacacg tggacgccga gcgcctgggc cgcgtcctcg tcctcgcgga
      301 tcgcgcgcca gtaatatccg gcgcgcgagt cgacgaggcg cgcgcacagc gccagggcgc
      361 cggcggtcag cgcgagcatc gcgtagtagt acagggcagg ggggccgcgg aagtggagca
      421 ggtcgatgcg atcgcgctcg gcgaccggca ggaacatgcc gcccggcccc ccggtccact
      481 ccatgtgatc gaagccgatg cgcgtgaact cggcgaacgc gatggtgagc agtgcgaagt
      541 agacgcccga gacgccgaac cggaacgcca gacagccaat gacgacgccg accagcgccg
      601 cgaccgcgat cgccaccggc aggccggccc acggcccgat cccgaggtgg taatacagcc
      661 cgcccgcggc gtaggcgccg atgcccacgt acagggcatg gccgagcgag agctggccgg
      721 caaaccccat catcacgttc cacgcctggc cggtgtaggc gaagtacagg atcagcgtgc
      781 cgaccgacag cgcgtaggga ccggcgagcc agggaaacac gatcgccagg acgagcagga
      841 tcgcgagcag gcgcgccgcc cgggtcacgg cttcctcccg aacaggccct gcggccggac
      901 gatcagcacg acgatcagca ggccgaagct cagcatgctc ttcgcggacg gcgagatgta
      961 gatcccggcg agcgcctcgg acacgccgat cagcacgccg ccgagcagcg cgccgagcat
     1021 cgagcccatt ccgccgacga tgacgatcac gaacgacagc agcgtgtact gcgggccgac
     1081 gctgggcgtg acgtcgaaca gcagcgtcag cgagcagccg gcgaccgcga cgcataccgc
     1141 gccgaggccg aacgtgatgg catacagccg cttgacgttc aggccgacca cccgggcggc
     1201 gaagtggttg tcggcgcacg cgcggatcgc cttgccggtc cgcgtgaagc ggaacacgcc
     1261 gatcagcgcg agcgcggtca gcaccgcgag ccccgcggca tacagccgcg ccttgtcgat
     1321 gacgatgtcg ccgagctcga acgactcgag ctggtagtcg acctgcacgc tgcgcgcgtc
     1381 ggggccgaac gccatgagca gcccgttgat cacgatcagc ccgaccgcga ccagcagcat
     1441 gaactgcgag tgctcgggcc gggtgatgaa ccggttgatc aggccggcct gcagcgcgta
     1501 gccgagcacg aagaacgcgg ccgcgaccgg gagcacggcg aggaacggat cgagctcgta
     1561 gtgcgcgaac agcagcgtcg cggcgtacat cgccacggtc atcagctcgc cgtgcgcgaa
     1621 gttgaccacc cggaccacgc cgaagatcac cgacaggccg agcgccatga ggccgtagac
     1681 cagccccgtc aagatcccgg cgaggacgcc ggcgacgacg aactcgaacg gcaacctatg
     1741 tcttcttgta gtccggccgc gggaacaccg gcttggcggt cgcggcggca gcgggcagca
     1801 ccacggtggg cagctggttc aggttctgga cgcacgccga gccgatgccc tcgacctggc
     1861 ccttggggtt gaacttgatc ggcccgccca gcgtcatctt gtcggtgatg tgggtctggc
     1921 ggatcgcctc ggccagcgcc ttggggtcgg tggtcctggc gcgcttgaac gcgtcggcgg
     1981 cgaccaggat cgcctcgaac gtgaagccga cgttcagccc gtggaacacc agcttgtcgt
     2041 tcgggttctg cttgacgaac gccttctcga cgatcttggt caggttggcg gtggggttga
     2101 accacggcac gttggagatc gagtactcgg ccagcttgcc gaggcccttg acgtactgct
     2161 cctcgtacat gcccggcgag ccggggctga tgatccccat cgggttccag cgctgcttga
     2221 ccatctcgcg gcgcaggagg atggcgtcgt tgagccgcga caccaggagc aggaagtcgg
     2281 ccttggcggc cttggcgcgc gacacctcga cggtgaggtc cttggccgcc ggatcgtagc
     2341 cgatgtcgtc gacgatcttg aagccgagct gcgagcgcga cggcagcatc gcggtgatcg
     2401 ccttggtcat cgcctgaccg aacgtgtcgt tgacgtgcat gtacaccgcg gtggtcggcg
     2461 cggcgtgggt ggtctggaac agctcgccga tcagatcgag cccgttcttc acgatgtcgc
     2521 cggcggtctg gaagttgcga aacacgaact tgtagccctg ctcggtgatc tgcggggccg
     2581 ccgcgatgtt gatgatcagc ggcacgccgc gctgctcggc gacctgggcg atcgcggaag
     2641 cggcgccgga gtcgaacgga ccgaccagga cgtgggcgcc gtcctggatc agccgctcgg
     2701 cgcgggtgcg ggccgtgtcg atgttcgact cgaagtcggc gttcatcagc tcgagctcga
     2761 cgccgagcat ctccttgatc acgccggggg cgaggtcggc gcccttctgg caggactgcc
     2821 cgatcagccc gagcgtcccc gagcgcggca ggatcacacc gaccttgagc ttcggcgcct
     2881 gcgcgcgggc gctgcgcagc ggcaagagcg acgaggcgag gccggtggcg agtacggact
     2941 ggaacgtgcg gcgcgtgatg ccctgggagc tggccatgaa tcctccgggt cggcccgacg
     3001 ttagcagagt tcgcggaccg gaaggctgcc gccgcgcgtc aggtctccgg cggctcgacc
     3061 ggcttgccgg cgagatcggt gttcgcgtcg aggcacagca ggcggacgcg cccggtcgag
     3121 cagatcttgc cggcgtcgtt ggtgatcgtg gcctgccaca cctgcgtgcg ccggccgcgg
     3181 gtcaccggcg tcgcggtgac gtgcagcgtg ccgtcgcgca ccgcggcgat gaacgacgtc
     3241 gagttctcga ccccgacgac cgactggccg cgggccagcg cgaccaccgc ggcgccggtc
     3301 gagcacgccg cctcgatgat ccccgcgtgc accccgccgt gcacgatccc gtacggctgg
     3361 cggtggatgt cggcgatcgc gacctcgccg atgcactcgt cggtcgtgac cttcgagaac
     3421 cgcagcccca tcgccgtgtt ccagcccccg agccgggtgt tgagcatcgc gctgaagtcg
     3481 gtcggttcgc tcatgatgcc cgtcgcatag cacacctgcg gaatccgccg cgacccggac
     3541 gagcgcggcc gggttcgggt acactgccgc gtgcagtgcc tggcaggacg cgccggctcg
     3601 tgccgctgat ccccgcgccc ggcgaccccg ccggtcggcg cgcgcaggcg cagcgcgacg
     3661 gctacctcta tctcccgcga ctgctccgcg actcgacgct ggccccgctc cgcgcgctgg
     3721 tcgagggcgc gctgacccgc cgcggctggc tgcgcggcgc gaccaccgat ccggcgctgc
     3781 gcctcggccg
//
LOCUS       DASVOR010001229         2384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421020,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001229 DASVOR010000000
VERSION     DASVOR010001229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421020"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(464..937)
                     /locus_tag="VF516_23355"
     CDS             complement(464..937)
                     /locus_tag="VF516_23355"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HEX8110697.1"
                     /translation="MTSRSCLLAPILAIALAACGGGATTAGPTTTAPPPPPSEPVPAA
                     ATTPETPAAPAAPARQMAPGPNLTADGVVFVFKPEGGGKKKVFLAGNFNDWKPDNPQY
                     LMTADATGAYTITVKLPPGTYQYKFVADGQWIKDPFSPGDAPDGFGGRNGKFDVK"
     gene            complement(1325..1810)
                     /locus_tag="VF516_23360"
     CDS             complement(1325..1810)
                     /locus_tag="VF516_23360"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX8110698.1"
                     /translation="MWDAPLHTLQGAPTRLADHKGKALLLVNVASQCGLTPQYTALEK
                     LHEKLAPRGFSVIGFPCNQFGAQEPGTPEQIQTFCSTTYGVTFPLTEKIEVNGAGRHP
                     IYKELTQVADAAGHKGDIRWNFEKFVVSADGAKITRFDPRTAPDDPAVLAAIEAGLPK
                     K"
     gene            complement(1843..>2384)
                     /locus_tag="VF516_23365"
     CDS             complement(1843..>2384)
                     /locus_tag="VF516_23365"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEX8110699.1"
                     /translation="NLPPAGAPPPPVNVDNLDSLWSAPIKTLQGKSTTLAAYKGKALM
                     LVNVASKCGNTPQYATLEALQKKYESKGFTVIGFPCNQFHGQEPGTAEEIATFCATNY
                     GITFPIMEKIEVNGDGRHPIYKTLTPIADAGGHEGDIRWNFEKFVVSADGKHITRFSP
                     KVKPEDPAVIGAVEAALPH"
BASE COUNT          344 a          745 c          871 g          424 t
ORIGIN      
        1 gatcttggtc tgcgctttcg tggtcagaca ccgccagcac ctatcagagc gaagatccga
       61 ctcgcgatcg cagaggcgat cgaacggcgt ctcgtggtgg aagcgggcac cgacctcgcg
      121 atcggcccac ttgccgagga aaggttccag acggcaccgg aagcagaagt agtctacgat
      181 accggatggc atcactccgg caattcgcat ccgatgcaag gttggacgga gctcgagcga
      241 atcgtcgagt tcgcaccgtc ggcgtcggca caaaacgtcg acgctgcact tcggcgcttg
      301 cgaaatggat gggaggcatt gcttgcccgt agccacgatg tgaaaccgaa gaagagcagc
      361 tcggcccagt tcgctggttg ctgcgcgttt ggcacgatca cacgagcatt tactgagcgt
      421 gggattgcac ttccggctca gcagccaaga gggtgtcggg cgcttacttc acgtcgaact
      481 tgccgttgcg gccgccgaag ccgtcgggcg cgtcgcccgg cgagaacgga tccttgatcc
      541 actggccgtc ggcgacgaac ttgtactggt aggtgccggg cggcagcttg acggtgatcg
      601 tgtacgcgcc ggtggcgtcc gcggtcatga ggtactgcgg gttgtcgggc ttccagtcgt
      661 tgaagttgcc cgcgaggaag accttcttct tgccgccgcc ctcgggcttg aacacgaaca
      721 ccacgccgtc ggcggtcagg ttggggccgg gcgccatctg cctggccggc gcagccggcg
      781 cggccggggt ctccggcgtc gtggcggcgg ccgggacagg ctcggagggc gggggcggcg
      841 gggccgtcgt tgtcgggccg gcggtggtcg cgccgccgcc gcaggcagcg agggcgatcg
      901 cgaggatcgg agcgagcagg caagatctcg aggtcatgag ggtctccagc gtcttgcggc
      961 gatactacga caacggcgtc atgccgggca cgatgcgagc accgggccga tctgccggcg
     1021 cggtggggtg acgagtgcgg tgaggctcgc agcgcccgct cggtgcgccg cgcgctcgtc
     1081 accgcgcgac tgccaccgca aaaccgcgct gctgggatcg ggcaggcatc catgtcgatc
     1141 gccgtgccag gttgacagtc tcgcgcggtg gagcgatgtc gggtgaacgc ggcgcgcggc
     1201 aagcctgcga gttccctgtg caccgtcggg ggccactggt tgcaacgccc tcctggcggt
     1261 gctgcagcac cacggcctgg agccaccacc gcgcgcccct gcggacccgg tgtgatgtct
     1321 tcggtcactt cttcggcagg ccggcctcga tcgccgcgag cacggcggga tcgtcgggcg
     1381 cggtccgcgg atcgaaccgc gtgatcttgg cgccgtcggc cgacacgacg aacttctcga
     1441 agttccagcg gatgtcgccc ttatggccgg cggcatcggc gacctgggtg agctctttgt
     1501 agatcgggtg gcggccggcg ccgttgacct cgatcttctc ggtcagcggg aaggtcacgc
     1561 cgtaggtcgt cgagcagaac gtctggatct gctccggcgt gccgggctcc tgggcgccga
     1621 actggttgca cgggaacccg atcaccgaga acccgcgcgg cgcgagcttc tcgtgcagct
     1681 tctcgagcgc ggtgtactgc ggggtcaggc cgcactgcga ggcgacgttg accagcagca
     1741 gcgccttgcc cttgtggtcg gcgagccggg tcggcgcgcc ctgcagcgtg tggagcggtg
     1801 cgtcccacat cgtggtcatg atggcctccg gagtcgctgg gctcagtgcg gcagggcggc
     1861 ctcgacggcg ccgatcaccg ccgggtcctc gggcttgacc ttgggcgaga accgcgtgat
     1921 gtgcttgccg tcggccgaga ccacgaactt ctcgaagttc cagcggatgt cgccctcgtg
     1981 gccgccggcg tcggcgatcg gtgtcagggt cttgtagatc gggtggcggc cgtcgccgtt
     2041 gacctcgatc ttctccatga tcgggaacgt gatgccgtag ttggtggcgc agaacgtggc
     2101 gatctcctcg gcggtgcccg gctcctggcc gtggaactgg ttgcacggga agccgatcac
     2161 ggtgaagccc ttggattcgt acttcttctg cagcgcctcg agcgtggcgt actgcggggt
     2221 gttaccgcac ttcgaggcga cgttgaccag catcagcgcc ttgcccttgt aggcggcgag
     2281 cgtggtcgac ttgccctgca gcgtcttgat cggcgcgctc cacagcgagt cgaggttgtc
     2341 gacgttgacc ggcggcggcg gcgcgccggc gggcggcagg ttcg
//
LOCUS       DASVOR010001230         2567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_333902,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001230 DASVOR010000000
VERSION     DASVOR010001230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_333902"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1433)
                     /locus_tag="VF516_23370"
     CDS             complement(108..1433)
                     /locus_tag="VF516_23370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110700.1"
                     /translation="MTPVGHHPLVGQLLLSVGASENEAPSLETWRDLLARMSQMYYET
                     DQDRDELESSIESSSREMQRLYQELKQKAEAERAEQDAILRATLESANEGILVVDNHQ
                     GVIAVNRRFVEMGRIPDEVVATHDRRTVIAAALTKVKMADAILARTDELYESDVTVRD
                     EIELVDGALMDWFSTPVRTPDGVKVGRVAFVRDVTAERHAQQQLQRAREAAEAASQAK
                     SQFLANMSHELRTPLTAVIGLSDLLLMERDPLVPRQKEYLEGVASSGRHLLALVNDVL
                     DLAKIEAGKTELKLESVPIHDAIEEGLTTISPLAVTRGVILEPATIVAVPNVRADRLR
                     LRQILYNLISNAVKFTDRGGRVRVRARRDDDRVSIAVMDTGIGIATRDLTRLYRSFEQ
                     LTLPSGDRPGGTGLGLALTKRLVEMHGGTIDVESELGVGTTFTVRIPVA"
     gene            1773..1846
                     /locus_tag="VF516_23375"
     tRNA            1773..1846
                     /locus_tag="VF516_23375"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1807..1809,aa:Met,seq:cat)
     gene            complement(1828..>2567)
                     /locus_tag="VF516_23380"
     CDS             complement(1828..>2567)
                     /locus_tag="VF516_23380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110701.1"
                     /translation="VAGGDELDQPADLVLRARQPVDALLAAGFDGPGVVAQLLRNALA
                     ELRDAALASLQPLAARGVRVEELRDYSRNIEARRKERVERLARTEDKIRGLIEFIATG
                     DRSEYVVATLRDLEAFARSEKAALAEIAQEANRPLRLPSIEEVTALATDLEARLTEDP
                     ELGREALRRWLHEGRIVVDQGPDGPTAQGELLPLMVLVDGSEKATRKSGGGGGGGAAG
                     TRKLEIPGDGSFSRDIKLLAMSSGGRI"
BASE COUNT          378 a          965 c          781 g          443 t
ORIGIN      
        1 atcgccgacg cccgccgcgc acagcacatc gtgaccgcgc agcacgagca gctcgaacac
       61 gagccggcgg ctccagagat cgtcttcgac aacgagaatg cgcgccatca ggccaccggt
      121 atcctcacgg tgaaggtcgt accgacgccg agctcgctct cgacgtcgat ggttccaccg
      181 tgcatctcca ccagtcgttt ggtcagtgcg agccccaacc ccgtaccccc cggacggtcg
      241 cccgacggca gcgtcagctg ctcgaacgag cggtacagtc gcgtcagatc tcgcgtcgcg
      301 atcccgatcc cggtgtccat caccgcgatc gacacccggt cgtcgtcgcg ccgggcgcgg
      361 acccggaccc ggccgccgcg atcggtgaac ttcaccgcgt tcgagatcag gttgtagagg
      421 atctggcgta gccgcagccg gtcggcgcgc acgttgggca ccgcgacgat cgtcgccggc
      481 tccaggatca ccccgcgcgt gaccgccagc ggcgagatcg tcgtcagccc ctcctcgatc
      541 gcgtcgtgga tcgggaccga ctccagcttg agctcggtct tgcccgcctc gatcttggcg
      601 aggtcgagca cgtcgttgac cagcgcgagc aggtgccggc cgctcgatgc gactccctcc
      661 aggtactcct tctgccgcgg caccagcgga tcgcgctcca tcagcagcag gtccgacagc
      721 ccgatcaccg cggtcagcgg cgttcgcagc tcgtgcgaca tgttcgcgag gaactggctc
      781 ttggcctggc tcgcggcctc cgctgcctcg cgcgcccgct gcagctgctg ctgggcgtgc
      841 cgctcggccg tcacgtcgcg aacgaacgcc acgcgcccca ccttcacgcc gtccggcgtc
      901 cggaccggcg tgctgaacca gtccatcagc gctccatcca ccagctcgat ctcgtcgcgg
      961 accgtcacgt cgctctcgta cagctcgtcg gtccgcgcca ggatggcatc ggccatcttc
     1021 accttggtca gcgccgccgc gatcaccgtg cgccgatcgt gcgtcgccac gacctcgtcc
     1081 gggatccttc ccatctccac gaaccgccgg ttcaccgcga tcacgccctg gtggttgtcg
     1141 accaccagga tcccctcgtt cgccgactcc agcgtcgcgc gcaggatcgc atcctgctcc
     1201 gcgcgctccg cctccgcctt ctgcttcagc tcctgataca gccgctgcat ctcgcgcgac
     1261 gagctctcga tcgagctctc cagctcatcg cgatcctgat cggtctcgta atacatctgg
     1321 ctcatccggg ccagcaggtc gcgccaggtc tccagcgacg gcgcctcgtt ctcgctggcg
     1381 ccgacgctga gcagcagctg accgacaagg ggatgatgcc cgacgggggt catgatgggg
     1441 cgaaacgata cagggcctgg cagtcgtaca gcctcgtcat ccaactacac catgatgccc
     1501 cggccgaggt cgcgcccctg ctgtggaagt cacagcaggt agcgctggga cgctggcaca
     1561 tcatccgcca ttgtctgatc cctcaccgtc cgacgcgctt gtcctcgctg ccgcgatttg
     1621 ctgcagtatt cgagcttacc gcctgccttg cgctggtttc tcctcatgtc ggtgcgccga
     1681 cgctcgcgtc cccgcggcct gccgcgaagt gcagccccag gccttgacgc ccggtgccct
     1741 gggggctaga aacactcgcg ctgacaactt gatgcggggt ggagcagcct ggtagctcgt
     1801 cgggctcata acccgaaggt cgcaggttca aatcctgccc ccgctactca tagctaacaa
     1861 cttgatatcc ctggagaagc taccatctcc agggatttcg agttttcggg tacctgcggc
     1921 gccccctccg ccaccccctc cggatttcct cgtggctttc tccgagccgt cgaccagcac
     1981 catcagcggc agcagctcgc cctgagccgt cggcccgtcc ggcccctgat ccacgacgat
     2041 ccggccctcg tgcagccacc ggcgaagcgc ctcccggccc agctccggat cctcggtcag
     2101 ccgcgcctcc aggtcggtcg ccagcgcggt cacttcctcg atgctcggca gacgaagggg
     2161 ccggttcgct tcctgggcga tttctgcgag ggctgccttc tcgctgcggg cgaacgcctc
     2221 caggtcgcgc agcgtcgcga cgacgtactc cgagcggtcg ccggtggcga tgaactcgat
     2281 cagcccgcgg atcttgtcct ccgtgcgcgc gagccgctcg acgcgctcct tgcggcgggc
     2341 ttcgatgttc cgggagtagt cgcgcagctc ctcgacccgc acgccgcgcg ctgcgagcgg
     2401 ctgcagggag gcgagcgcgg cgtcgcgcag ttccgcaagc gcattgcgga ggagctgcgc
     2461 gactactccc ggtccatcga agcccgccgc aaggagcgcg tcgaccggct ggcgcgcacg
     2521 gaggacaaga tccgcgggct gatcgagttc atcgccaccg gcgaccg
//
LOCUS       DASVOR010001231         7397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_129291,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001231 DASVOR010000000
VERSION     DASVOR010001231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7397
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129291"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VF516_23385"
     CDS             <1..1426
                     /locus_tag="VF516_23385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEX8110702.1"
                     /translation="VDVIDAVAVVLGEPVEPVVASGAKIVDQINKTYSRLRGSHELEE
                     GAKKDDEEDSEYASEELVDMLDANDEAPIIRWVNSLMFQAAKERASDIHIEPGEKDVV
                     VRYRVDGVLREAKRAPKKFQASIIARVKIMAGLNIAEKRLPQDGRIRRKMAGKDVDMR
                     VATAPTAGGERITIRLLDRSSVLLGLADIGFADDHLVLIRGIIKRPHGILLVTGPTGS
                     GKTTTLYACLSEINAPDINILTVEDPVEYQLEGISQTQVNPKIDLTFASGLRSFLRHD
                     PDVIMVGEIRDRETAEVAITASLTGHLVFSTVHTNDAAGGITRLIDMGIEPFLVASSL
                     VGLLAQRLVRRPCYECARPVRPSEEILRELGLDPRTFFTGGYVLPPVKGEHPPPPGTV
                     YEPVGCGACGELGYRGRTGIYELLMVSDQVRRLCLDKSDAGSIRNAAIEHGMVTLRFD
                     GARKVVQGLTTPEEVMLMTAESGD"
     gene            1426..2682
                     /gene="gspF"
                     /locus_tag="VF516_23390"
     CDS             1426..2682
                     /gene="gspF"
                     /locus_tag="VF516_23390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HEX8110703.1"
                     /translation="MPMYAYKGVGASGKAVTGVRDAESPKVLRQVLRKDGVLVMSFEL
                     SRGGKLAKEANAKKGGLSRDVDLGGLIGGVRKVEIAAFTRQMATLIKAGIPLADALGA
                     LVEQLTNVRFKAPVSEVRAAVNEGSSLADALARHPKLFDDLFVSMVRAGEVAGNLDEV
                     LARLADFLEGSQKLKSKVQSAMIYPLVMVVVGAIIMSVLMIKVIPNITDMFKQQGKTL
                     PINTRMLIASSDFIRNWWWAVIFLVIAGIVLFLQWTKGKEGRMVWDRFTLRLPVVGEL
                     VRTINVSRFARTLGTMLHSGVPMLRALDTAKQIVGNVLIQQAIEDAKRAVTEGESLAT
                     TLKKSGQFPATMVHMVAVGEKAGQLEQMLERVADTYESEVDTKLTRLTALLEPVMLVV
                     MGGAVAFIVFSILQPIMDLGQLSGPH"
     gene            2712..3188
                     /locus_tag="VF516_23395"
     CDS             2712..3188
                     /locus_tag="VF516_23395"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8110704.1"
                     /translation="METLRNRLSRLSRVLARVLARAQARAQARAQRGMTLLEIMIVLA
                     ILALVMGLLVGPKVMKMFAGSKVDITKIKLKKYAYEAYPSWSAAHPDKACPGNISELN
                     EYMNDTSSNASNDAWGRPIRMMCGGNMPAGAKGIALVSLGEDGKEGTEDDLKSWEE"
     gene            3235..4029
                     /locus_tag="VF516_23400"
     CDS             3235..4029
                     /locus_tag="VF516_23400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEX8110705.1"
                     /translation="MTASSPPPRARTASGQSGLTVLEIMIVLAIVGGLFYVVRSGFRL
                     VTKADLVEDATELTAVMRRASQLAVEHGEMHRVTLDLDAQAYAVEVCQGAVGIARNEL
                     TKADDEATKRALEKGRQRLAGLPPDALATGDPEEATKRAAALAGHHVADRTCKPADNS
                     VTGDSNGKGWIRKLRKDKGVKFKEVWVAHRDDSATKGQIAIYFFPIGGAEKAVIELTD
                     GSEVFSVLVFGLTGRVELHDGQLRDVNDHMLKNAMGDRDTKRETQN"
     gene            4029..4820
                     /locus_tag="VF516_23405"
     CDS             4029..4820
                     /locus_tag="VF516_23405"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEX8110706.1"
                     /translation="MRAATTTASPAPAARRGFTLLEVMLGLALLGLGLTVLIKSTASD
                     IFNAQQAHLMGVVTDLARGKMYDIEEKLLHDGFSDTEQHEDDKNFSDEGWPQIHYSYK
                     VEVVELPGFEQLQAIIAGTGSGSARSAAGSDGEAAGGFENSMLGGMMAQFGGLGGGGG
                     GKGSADINASIGGAFVQSQYSMFQQILKVTIRKITLTVNYQVMGSDRDLKVVQFLTDA
                     AAMDKVLNGMGANELPAAGSGSSGSNSPPTTKPPTTPPTQPPKKP"
     gene            4820..5512
                     /locus_tag="VF516_23410"
     CDS             4820..5512
                     /locus_tag="VF516_23410"
                     /inference="COORDINATES: protein motif:HMM:NF023044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspJ"
                     /protein_id="HEX8110707.1"
                     /translation="MRGRRSPQAGLTLLEVMLAIAILAVMMTLAWKTISNTSDTKKTF
                     EAYEERNHELRMALGRIVRDFEHAYLSQNEDRNAAHPRTMFIAKSSMRLPDVRFSTLD
                     HRVLWADANESEQTVISYVPHDNPDHPGAVDWIRREQRRMSNEPPESEPADYDVLVHD
                     IAAAKLEFWNWKNNDWQDSWDTTQADGQKGWLPSRVRITITIKGPDDKDVKLTSQARI
                     WMQEVLSTLITQ"
     gene            5529..6917
                     /locus_tag="VF516_23415"
     CDS             5529..6917
                     /locus_tag="VF516_23415"
                     /inference="COORDINATES: protein motif:HMM:NF045433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110708.1"
                     /translation="MTLLTAIKDALLRERTLGRRPASPARRKRNRQRGVALFAVMIAM
                     AIVLVFTSEFGTSTNVDLMAAANYRDQMRAHFLARSALNLSELVIRVQQRLDNTQIGG
                     TRLSDMGIRITDFADQVLLAFCGNPEQVQSMVGFSSSDVKGLGVDIGTCGIVNRSITT
                     DDDKINLNCANFGKTPEDVLKSTLDALVYFPAYDPVFEEADAENYRRDRATQVAALLD
                     YVDADSVHVHDIGTTEDYGYESLKDKYKPKNNYLDTVGEIRLARGVDDRFWTLFGPAF
                     TVYGGCKVNLSAVSNTQLIAAILYLSAKNPNDPMLQDPLKLFALASVVAQAKQFGETF
                     NKLSDFADFVKDPTAMIGLLASKSGTLAGSAAAQSVSTGAFGALMGGQKLGMELDTNK
                     LGQIAAAGPRRTYRVEAFGEIERKQKAADGSPVFPAIRATVTGVWDTKVVPQNVRKPP
                     VPKGAWVFLRED"
     gene            6920..>7397
                     /locus_tag="VF516_23420"
     CDS             6920..>7397
                     /locus_tag="VF516_23420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110709.1"
                     /translation="MASRVFAVDLGAWSVKLAIASPGLRGATLYNVVERPVPPGDEPA
                     EVRARAVLAGLVGELRLRDDAGYLGVAGDQVFTQILEFGFKNLRRAELDKAVGGELEG
                     VVPVDLEDMVYAFEPLPPPVTPAAGAGPGPGPMPMPIPDLQRGRVAAPPEGMRVLTY"
BASE COUNT         1284 a         2534 c         2530 g         1049 t
ORIGIN      
        1 ggtcgacgtg atcgacgcgg tcgcggtcgt gctcggcgag ccggtcgagc cggtggtcgc
       61 gtcgggcgcc aagatcgtcg accagatcaa caagacctac tcgcggctgc gcggcagcca
      121 cgagctcgag gagggcgcca agaaggacga cgaggaggac agcgagtacg cgtcagaaga
      181 gctggtcgac atgctcgacg ccaacgacga ggcgccgatc atccggtggg tcaactcgct
      241 gatgttccag gccgccaagg agcgcgcgtc ggatatccac atcgagcccg gcgagaagga
      301 cgtggtcgtc cgctaccggg tcgacggcgt gctgcgcgag gccaagcgcg cgcccaagaa
      361 gttccaggcg tcgatcatcg cgcgcgtcaa gatcatggcc gggctcaaca tcgccgagaa
      421 gcggctgccg caggacggcc ggatccggcg caagatggcc ggcaaggacg tcgacatgcg
      481 cgtcgcgacc gcgccgaccg ccggcggcga gcggatcacg atccgtctgc tcgaccgcag
      541 ctcggtgctg ctcggcctgg ccgacatcgg gttcgccgac gaccacctgg tgctgatccg
      601 cggcatcatc aagcggccgc acggcatcct gctggtcacc gggccgaccg gctcgggcaa
      661 gaccaccacg ctgtacgcct gcctgtccga gatcaacgcg ccggacatca acatcctcac
      721 cgtcgaggat ccggtcgagt accagctcga gggcatctcg cagacccagg tcaaccccaa
      781 gatcgacctg acgttcgcct cggggctgcg ctcgttcctc cgccacgatc ccgacgtgat
      841 catggtcggc gagatccgcg accgcgagac cgcggaggtc gcgatcaccg cgtcgctgac
      901 cggccacctg gtgttctcga ccgtccacac caacgacgcc gccggcggca tcacccgctt
      961 gatcgacatg gggatcgagc cgttcctggt cgcgtcgtcg ctggtcggcc tgctcgccca
     1021 gcgcctggtc cggcggccgt gctacgagtg cgcgcggccg gtgcggccgt ccgaggagat
     1081 cctgcgcgag ctcgggctcg atccgcggac gttcttcacc ggcggctacg tgctcccgcc
     1141 ggtcaagggc gagcacccgc cgccgccggg cacggtgtac gagccggtcg gctgcggcgc
     1201 gtgcggcgag ctcggctacc gcggccggac cgggatctac gagctgctga tggtgagcga
     1261 ccaggtgcgc cggctgtgcc tcgacaagtc cgacgccggc tcgatccgca acgccgcgat
     1321 cgagcacggc atggtgacgc tgcggttcga cggcgcgcgc aaggtggtcc agggcctgac
     1381 gacccccgag gaagtcatgc tgatgaccgc ggagagcggt gactgatgcc gatgtacgcg
     1441 tacaagggcg tcggcgcctc gggcaaggcg gtgaccggcg tgcgcgacgc cgagtcgccc
     1501 aaggtgctgc gccaggtgct gcgcaaggac ggcgtgctgg tcatgagctt cgagctgtcg
     1561 cgcggcggca agctcgccaa ggaggcgaac gccaagaagg gtgggctgtc gcgcgacgtc
     1621 gacctcggcg ggctgatcgg cggtgtccgc aaggtcgaga tcgccgcgtt cacccggcag
     1681 atggcgacgc tgatcaaggc cggcatcccg ctggccgacg cgctcggcgc gctggtcgag
     1741 cagctcacca acgtccggtt caaggcgccg gtgtccgagg tccgcgccgc ggtcaacgag
     1801 ggcagctcgc tggccgacgc gctggccagg caccccaagc tgttcgacga cctgttcgtg
     1861 tcgatggtgc gcgccggcga ggtcgccggc aacctcgacg aggtgctggc acggctcgcc
     1921 gacttcctgg agggctcgca gaagctcaag tccaaggtcc agagcgcgat gatctatccg
     1981 ctcgtcatgg tcgtggtcgg cgcgatcatc atgagcgtgc tgatgatcaa ggtgatcccg
     2041 aacatcaccg atatgttcaa gcagcagggc aagaccctgc cgatcaacac ccggatgctg
     2101 atcgcgtcgt cggacttcat ccgcaactgg tggtgggcgg tgatcttcct ggtcatcgcg
     2161 ggcatcgtgc tgttcctgca gtggaccaag ggcaaggagg ggcgcatggt gtgggaccgc
     2221 ttcacgctgc gcctgccggt ggtcggcgag ctggtccgca cgatcaacgt cagccggttc
     2281 gcgcgcacgc tgggcacgat gctgcactcc ggcgtgccga tgctgcgcgc gctcgacacc
     2341 gccaagcaga tcgtcggcaa cgtgctgatc cagcaggcga tcgaggacgc caagcgcgcg
     2401 gtcaccgagg gcgagtcgct cgcgaccacg ctcaagaagt cgggccagtt cccggcgacg
     2461 atggtccaca tggtggcggt cggcgagaag gccggccagc tcgagcagat gctggagcgc
     2521 gtcgccgaca cctatgagtc cgaggtcgat accaagctga cgcggctcac cgcgctgctc
     2581 gagccggtga tgctcgtcgt catgggcggc gcggtcgcgt tcatcgtgtt ctcgatcctg
     2641 caaccgatca tggacctcgg tcagctgtcc ggtccgcatt aagtctgtac aaggagctca
     2701 aggagatcgt catggaaacg ctacgcaacc gcctgtcccg tctgtctcgt gtcctggctc
     2761 gcgtcctggc tcgcgcccag gcccgcgccc aggctcgcgc ccagcgcggc atgacgctgc
     2821 tggagatcat gatcgtcctc gcgatcctcg cgctggtgat gggactcctg gtcgggccca
     2881 aggtcatgaa gatgttcgcc gggtccaagg tcgacatcac caagatcaag ctcaagaagt
     2941 acgcctacga ggcctacccg tcgtggtcgg cggcgcaccc cgacaaggcg tgcccgggca
     3001 acatctccga gctcaacgag tacatgaacg acacctcctc gaacgcctcg aacgacgcct
     3061 ggggccggcc gatccggatg atgtgcggcg gcaacatgcc ggccggcgcc aagggcatcg
     3121 ccctggtgtc gctgggcgag gacggcaagg aaggcaccga ggacgatctg aagtcgtggg
     3181 aggagtgagc ccgctccgcg agccaccacc accaccaccg ccaccggcac tgcgatgacc
     3241 gcatccagcc cacctccgcg cgcgcgcacg gcctccggcc agtccgggct gaccgtgctc
     3301 gagatcatga tcgtgctcgc gatcgtgggc gggctgttct acgtcgtgcg ctcggggttc
     3361 cggctggtga ccaaggcgga cctggtcgag gacgccaccg agctgaccgc ggtgatgcgg
     3421 cgggcgagcc agctcgcggt cgagcacggc gagatgcacc gcgtgacgct cgatctcgat
     3481 gcccaggcct acgcggtcga ggtctgccag ggcgcggtcg ggatcgcgcg caacgagctg
     3541 accaaggccg acgacgaggc gaccaagcgc gcgctggaga agggccggca gcggctcgcc
     3601 ggcctgccgc ccgacgcgct ggcgaccggc gatccggagg aggcgaccaa gcgcgccgcc
     3661 gcgctggccg ggcaccacgt cgccgaccgg acgtgcaagc cggccgacaa cagcgtcacc
     3721 ggcgactcca acggcaaggg ctggatccgc aagctgcgca aggacaaggg cgtcaagttc
     3781 aaggaggtct gggtcgcgca ccgcgacgac agcgcgacca agggccagat cgcgatctac
     3841 ttctttccga tcggcggcgc cgagaaggcg gtgatcgagc tgaccgacgg cagcgaggtg
     3901 ttcagcgtgc tggtgttcgg gctgaccggc cgcgtcgagc tgcacgacgg ccagctgcgc
     3961 gacgtcaacg accacatgct caagaacgcg atgggcgacc gggacaccaa gcgggagacc
     4021 cagaactgat gcgggccgcg acgaccaccg cttccccggc gcctgccgcg cggcgcggct
     4081 tcaccctgct cgaggtcatg ctcggcctcg cgctgctcgg cctcgggctc accgtgctga
     4141 tcaagagcac cgccagcgat atcttcaacg cccagcaggc gcacctgatg ggggtcgtca
     4201 ccgacctcgc gcgcggcaag atgtacgaca tcgaggagaa gctcttgcac gacgggttct
     4261 ccgacaccga gcagcacgag gacgacaaga acttctccga cgagggctgg ccgcagatcc
     4321 actactcgta caaggtcgag gtggtcgagc tgcccgggtt cgagcagctc caggcgatca
     4381 tcgccggcac cggctcgggc tcggcgcgca gcgctgccgg ctccgatggc gaagcggccg
     4441 gtgggttcga gaacagcatg ctcggcggca tgatggcgca gttcggcggg ctcggcggcg
     4501 gcggcggcgg caagggcagc gcggacatca acgcgtcgat cggcggggcg ttcgtgcaga
     4561 gccagtactc gatgttccag cagatcctga aggtgacgat ccgcaagatc acactgaccg
     4621 tgaactacca ggtcatgggc agcgatcgcg atctcaaggt cgtgcagttc ctcaccgacg
     4681 ccgccgcgat ggacaaggtg ctcaacggca tgggcgcgaa cgaactcccc gctgcgggct
     4741 cgggatcgag cggtagcaat agcccaccca cgaccaagcc gcctaccact ccccccacgc
     4801 aacccccgaa gaagccatga tgcgcggacg ccgctccccc caggccggac tgaccttgct
     4861 cgaggtcatg ctcgcgatcg ccatcctcgc cgtgatgatg acgctggcgt ggaagacgat
     4921 ctcgaacacc agcgatacca agaagacgtt cgaggcctac gaggagcgca accacgagct
     4981 gcgcatggcg ctcggccgga tcgtgcgcga cttcgagcac gcctacctgt cgcagaacga
     5041 ggaccgcaac gcggcccacc cgcgcacgat gttcatcgcc aagtcgagca tgcggctgcc
     5101 tgacgtccgg ttctcgaccc tcgaccaccg cgtgctgtgg gccgacgcca acgagtcgga
     5161 gcagaccgtg atctcgtacg tcccgcacga caaccccgac caccccggcg cggtcgactg
     5221 gatccggcgc gagcagcgcc ggatgtcgaa cgagccgccc gagagcgagc ccgcggatta
     5281 cgacgtgctg gtccacgaca tcgccgccgc caagctcgag ttctggaact ggaagaacaa
     5341 cgactggcag gacagctggg acaccaccca ggccgacggc cagaagggct ggctgcccag
     5401 ccgggtccgg atcacgatca cgatcaaggg ccccgacgac aaggacgtca agctgaccag
     5461 ccaggcccgg atctggatgc aggaagtgct gtccacgctc atcacccagt gaccccccag
     5521 tgacctcgat gaccctcctc accgcgatca aagacgcctt gctccgcgag cgcacgctcg
     5581 gccggcgccc ggcgagcccg gcccggcgca agcgcaaccg gcagcgcggc gtcgcgctgt
     5641 tcgccgtgat gatcgcgatg gcgatcgtgc tggtgttcac cagcgagttc gggacctcga
     5701 ccaacgtcga cctgatggcg gcggccaact accgcgatca gatgcgcgcc cacttcctcg
     5761 cgcgctcggc gctcaacctg tccgagctgg tgatccgggt ccagcagcgg ctcgacaaca
     5821 cccagatcgg cggcacccgg ctgtccgaca tggggatccg gatcaccgac ttcgcggacc
     5881 aggtgctgct cgcgttctgc ggcaaccccg agcaggtcca gtcgatggtc gggttctcgt
     5941 cgagcgacgt caagggcctt ggggtcgaca tcgggacgtg cggcatcgtc aaccggtcga
     6001 tcacgaccga cgacgacaag atcaacctca actgcgccaa cttcggcaag acgcccgagg
     6061 acgtgctcaa gagcacgctc gacgcgctgg tctacttccc ggcctacgac ccggtgttcg
     6121 aggaggccga cgcggagaac taccggcgcg accgcgcgac ccaggtcgcc gcgctgctcg
     6181 attacgtcga cgccgacagc gtgcacgtcc acgacatcgg cacgaccgag gactacggct
     6241 acgagagcct caaggacaag tacaagccga agaacaacta cctcgacacg gtcggcgaga
     6301 tccggctggc ccgcggggtc gacgatcggt tctggacgct gttcggcccg gcgttcacgg
     6361 tgtacggcgg ctgcaaggtc aacctcagcg cggtgtcgaa cacccagctg atcgcggcga
     6421 tcctgtacct gtcggccaag aaccccaacg atccgatgct ccaggatccg ctcaagctgt
     6481 tcgcgctcgc cagcgtggtc gcccaggcca agcagttcgg cgagacgttc aacaagctgt
     6541 ccgacttcgc cgacttcgtg aaggatccga cggcgatgat cggcctgctc gccagcaagt
     6601 ccggcacgct ggccggcagc gccgcggccc agtcggtgag caccggcgcg ttcggcgcgc
     6661 tgatgggcgg ccagaagctc ggcatggagc tcgacaccaa caagctgggc cagatcgccg
     6721 cggccgggcc gcgccggacc taccgggtcg aggcgttcgg cgagatcgag cgcaagcaga
     6781 aggccgccga cggctcgccg gtgttccccg cgatccgcgc gacggtcacc ggcgtctggg
     6841 ataccaaggt cgttccgcaa aacgtccgga agccacccgt gcccaagggg gcgtgggtgt
     6901 tcctgaggga agactgaaca tggcgagtcg cgtcttcgca gtcgatctcg gcgcatggag
     6961 cgtcaagctg gcgatcgcca gccccggcct ccggggcgcg acgctgtaca acgtggtcga
     7021 gcgcccggtg ccgcccggcg acgagccggc cgaggtccgg gcccgggccg tgctcgccgg
     7081 gctggtcggc gagctgcggc tgcgcgacga tgcgggctac ctcggcgtcg ccggcgacca
     7141 ggtgttcacc cagatcctcg agttcgggtt caagaacctg cgccgcgccg agctcgacaa
     7201 ggcggtcggc ggcgagctcg agggcgtcgt gccggtcgac ctcgaggaca tggtctatgc
     7261 gttcgagccg ttgccgccgc cggtcacgcc tgccgcgggc gcggggccgg gccccggtcc
     7321 gatgccgatg ccgatccccg acctccagcg cggccgggtc gcggcgccgc ccgagggcat
     7381 gcgcgtgctc acctatg
//
LOCUS       DASVOR010001232         6800 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_538711,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001232 DASVOR010000000
VERSION     DASVOR010001232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538711"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1713)
                     /locus_tag="VF516_23425"
     CDS             complement(<1..1713)
                     /locus_tag="VF516_23425"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8110710.1"
                     /translation="MSSPAERYAAARRRAERPALTDFRAGYPFELDPFQTEACEALED
                     GLGVLVCAPTGAGKTVVGEFAVHLALADGRKCFYTTPIKALSNQKYNDLVDKYGPGQV
                     GLLTGDNSVNGDAPVVVMTTEVLRNMLYAGSGAIDNLGYVVMDEVHYLADRFRGAVWE
                     EVIIHLPASVRLVSLSATVSNAEEFADWLVTVRGDTKVIVDEHRPVPLWQHMLVGTRI
                     FDLFRGAETGKPSRGGETGKEVDPELVRYVRERERYVEPVRRGGRSRGWRPPARVDVI
                     DRLDRDGLLPAITFIFSRAGCDAAVQQCVAAGLRLNTPAEAAEVREVAEEYTAELAPD
                     DLDVLGYWEWVDGLQRGVAAHHAGLIPTFKEAVEELFARGLVKAVFATETLALGINMP
                     ARSVVLERLVKFNGETHADVTPGEYTQLTGRAGRRGIDVEGHAVVLWSPGVNPEQVAG
                     LASTRTYPLRSSFRPSYNMAVNLSRQLSRPQARELLERSFAQFQADRAVVGLARQVDR
                     NQEALAGYAESMRCHLGDFAEYFALRQRLKERESALAREGAAARRAAAEQSLERLRPG
                     DVIRV"
     gene            1810..2379
                     /locus_tag="VF516_23430"
     CDS             1810..2379
                     /locus_tag="VF516_23430"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8110711.1"
                     /translation="MAYGIGGHDELLSTGAAAQLLGSCRQHVVDMCSRGDLPYVWVGR
                     HRRIRKSAIASIARHEREELTRDQERSLWLHRAVLAQLMSRPTDVLDLARQNALRVLQ
                     QQHRVSMTSSWLEQWLRILDNGVDDVAETLTSRASLAVELRQNSPFAGVLPQDTRSKV
                     LAAFAQHWREEHGSHSVDVRRRTSTPSGD"
     gene            2369..2938
                     /locus_tag="VF516_23435"
     CDS             2369..2938
                     /locus_tag="VF516_23435"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEX8110712.1"
                     /translation="MATEQVNREQLAHVLRAASAIVDDHDILIIGSQSLLGSFDENVL
                     PLEATASMEVDLAYFDDPDEVKADRVDGAIGELSPFHELHGFYAQGVSVSTAVLPEGW
                     RDRVVLWSNRSTGGARAAFLEPHDCVVSKLVAYREKDVAFAAALLDAGLVDATVLRSR
                     VDQLPAGVDRRIPRMLHDWLDWYVRSPRS"
     gene            complement(2941..6111)
                     /locus_tag="VF516_23440"
     CDS             complement(2941..6111)
                     /locus_tag="VF516_23440"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEX8110713.1"
                     /translation="MVSEAEWEEIALDRLFEHGWETLHGNQVAPGVEGGRTSWEDIVL
                     RGRLLASMRRLNPMVPGQYIEQALAEIVAPTSQDAIAENFRLHQILVDGYRGISYVDA
                     DGIEQTPTIRLVSHLVEDNEFLAVNQVTVRSSEVERRFDIVLYLNGMPVVIVELKKAG
                     AERADLASAHAQLATYIREFPMAFRFCMLSIISDGITARYGTPFTPLNHYSPWNVDDD
                     GRPRTPVTDVSDMSLGVELEYLIDGVCNQERFLQLQRNFVAFDAGAEGYAKRIAKPHQ
                     YFAVTKAVGSTVDAVESNGKAGVIWHTQGSGKSMEMELYAHLVSLQPKLKNPTLVVVT
                     DRTELDSQLFETFNRSQLLAESPVKVTTREQLRDELTHRTTGGIYFTTLQKFGLTDDE
                     RHAGLDHPLLTDRRNIIVIVDEAHRSHYDDLDGFARHIRDALPNAAFIAFTGTPISFA
                     DRNTQEVFGDVIDTYDLTRAVEDGATVPVYFEPRLISVKLAGEVTEEQLDEAADEATV
                     GLDDVERDRIEKSVAVINAVYGAPARLAALATDILHHWDQRSEAMRPFISSPGKAFIV
                     GATREICANLYEELVRLRPAWHDDAVDKGVIKVVYSGSAGDTGLVAKHVRRDALNKVI
                     QKRLKDAEDPLQIVIVKDMMLTGFDAPPLHTLYVDRPLKGALLMQTLARVNRTFRGKP
                     SGLLVAYAPLADNLNKALAEYTKTDRTKQPVGRNVDEAAVLARSLIEALDQICSGYPW
                     RKQLLGDTRSWIKAAVGLTNHLRSPSTPGNQVADGEETVAQRFRKLANQLARAWALAG
                     GRETLEDLRPIARFYEEVRVWMGKFDAQERQASGEPVPEEIQRLLSGLVASSTATGEI
                     VDIYQAAGLPKPSLSDLGPEFQLKAEKAENPHLAIEALRDLLTEESGRVARHNLVRQR
                     AFSERIKELMNKYTNQQLTSAEVIAELIELAKEVVAEANRGRQFTPPLSEDELAFYDA
                     VSTNESAVALQGEDVLAQIARELVEVMRRDVKTDWMVRDDVRAKLRSSIKRLLVKHKY
                     PPDKQPGAIKLVIEQMEAMAPRYVGV"
     gene            complement(6111..>6800)
                     /locus_tag="VF516_23445"
     CDS             complement(6111..>6800)
                     /locus_tag="VF516_23445"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEX8110714.1"
                     /translation="AANVRQLELSQNLADSIFVRAAGVLSEAALTFGDVADVGGGGTP
                     RTSVDEYWGGDIPWATPTDVTALAAPYLSGTARRITAARLAACASSVYPAGSILMTSR
                     ATIGAFAVAEVATAVNQGFIVVNAKEPVYQWWLFHEMRFRVQDFLSYANGATFLELPR
                     GRFKSLEVRLPAVEMAREFDRTVGPLHARAAVLLKENGRLASTRDELLPLLMSGRVRV
                     RDAEKVVGGVV"
BASE COUNT         1054 a         2344 c         2239 g         1163 t
ORIGIN      
        1 caccctgatc acgtcgccgg gccggagccg ttccagcgac tgctcggccg cggcgcgccg
       61 ggcggcggcg ccctcccggg ccagcgccga ctcgcgctcc ttgagccgct gccggagcgc
      121 gaagtattcg gcgaagtcgc cgaggtggca gcgcatcgac tccgcgtagc cggccagcgc
      181 ctcctggttc cggtccacct gccgggccag cccgaccacc gcccggtcgg cctggaactg
      241 cgcgaacgaa cgctccagca gctcgcgcgc ctgcggccgg gacagctgcc gggacaggtt
      301 gacggccatg ttgtacgagg gccggaacga cgagcgcagc gggtaggtgc gggtcgaggc
      361 cagcccggcg acctgctccg ggttgacgcc cggcgaccag agcacgaccg cgtgcccctc
      421 gacgtcgatg ccacgccggc cggcccgccc ggtcagctgg gtgtactccc ccggcgtcac
      481 gtccgcgtgc gtctcgccgt tgaacttgac cagccgctcc agcaccaccg accgggccgg
      541 catgttgatg cccagcgcca gcgtctcggt cgcgaagacg gccttgacca ggccgcgggc
      601 gaacagttcc tcgaccgcct ccttgaacgt cgggatcagc ccggcgtggt gcgcggccac
      661 cccgcgctgc agcccgtcga cccactccca gtagccgagc acgtccaggt cgtccggggc
      721 gagctcggcc gtgtactcct cggccacctc gcggacctca gccgcctcgg ccggggtgtt
      781 caaccggagc ccggcggcca cgcactgctg cacggccgca tcgcagccgg cccggctgaa
      841 gatgaacgtg atcgccggca gcaggccgtc gcggtcgagc cggtcgatga cgtcgacgcg
      901 ggccggcggg cgccagccgc gggaccggcc gccgcgccgc accggctcga cataccgctc
      961 ccgctcgcgg acgtaccgga cgagctcggg gtcgacctcc ttgcccgtct ccccgcccct
     1021 actgggcttg cccgtttccg ctccgcggaa gaggtcgaag atccgggtgc cgaccagcat
     1081 gtgctgccag agcggcaccg gccggtgctc gtcgacgatc accttggtgt cgccgcggac
     1141 ggtgaccagc cagtcggcga actcctcggc gttgctcacc gtcgccgaca ggctcaccag
     1201 ccggaccgac gccggcaggt ggatgatcac ctcctcccag accgcgccgc ggaaccggtc
     1261 ggccaggtag tgcacctcgt ccatgaccac gtagccgagg ttgtcgatcg cgccggagcc
     1321 ggcgtagagc atgttgcgca gcacctcggt ggtcatcacc accacgggcg cgtcgccgtt
     1381 gaccgagttg tcgccggtca gcaggccgac ctggcccggc ccgtacttgt cgaccaggtc
     1441 gttgtacttc tggttggaca gcgccttgat cggcgtggtg tagaagcact tgcggccatc
     1501 ggccaacgcc aggtgcacgg cgaactcgcc gaccacggtc ttgccggccc cggtcggcgc
     1561 gcagacgagc acgccgaggc cgtcctcgag cgcctcgcac gcctcggtct ggaacgggtc
     1621 gagctcgaac gggtaaccgg ccctgaagtc ggtcagcgcc ggccgctccg cccgccgccg
     1681 ggccgccgcg taccgctcgg ccggactgct caccctccca gcgtacggac cgaggatctc
     1741 ttggcggtcc ggtgcctctc gtccccaacg cccattgcgt ttttggtggt taggcgggat
     1801 catcgatgca tggcgtacgg gatcgggggg cacgacgagc tgctgtcgac gggagcggcc
     1861 gcccaattgc tgggctcctg ccgccagcac gtcgtcgaca tgtgcagccg tggcgatctg
     1921 ccgtacgtct gggtcggcag gcatcgacgc atccggaagt cggcgatcgc gtcgattgct
     1981 cgccacgagc gggaggagct cacccgggat caggagcggt cgctgtggct gcaccgggcc
     2041 gtcctggctc agttgatgag ccgaccgaca gatgtcctcg accttgcccg gcagaacgcg
     2101 ctccgtgtcc tccagcaaca gcaccgggtc agcatgacgt cgagttggct cgagcagtgg
     2161 ctgcggatcc tggacaacgg agtggacgac gtcgcggaga ccctgacgtc tcgggcttcg
     2221 ctcgccgtag aactgcggca gaactccccc ttcgccggag tgttgcccca ggacacccgc
     2281 tcgaaggtgc tggccgcatt cgcacaacac tggcgcgagg aacacggatc ccactccgtc
     2341 gacgtacgcc ggcgtacgtc gaccccgagt ggcgactgag caggtgaaca gggaacagct
     2401 ggctcacgtc ctgcgcgcag cctccgccat cgtcgacgac cacgacattc tgatcatcgg
     2461 aagccagtcg ctgctcggca gcttcgacga gaacgtgctg cctctcgaag ccaccgcctc
     2521 catggaagtc gacctggcct atttcgacga cccggacgag gtgaaggcgg acagggtcga
     2581 cggcgcgatc ggcgagctgt cgcctttcca tgagctacat ggcttctacg cccaaggggt
     2641 cagcgtcagc acggccgtcc tcccagaagg ctggcgcgac cgggtcgtgt tgtggtccaa
     2701 ccgatccacc ggcggggcac gcgccgcctt cctcgagccg cacgattgcg tggtgtcgaa
     2761 gctggtcgct taccgtgaga aggacgtcgc gttcgctgcg gccctcctcg acgcggggct
     2821 ggtcgatgcc accgtcctcc gtagccgcgt cgatcaactc cccgccggag tcgaccggcg
     2881 gatcccacgc atgctgcacg actggctcga ctggtacgtc cgaagccctc ggtcctgaac
     2941 tcacactccc acataccgag gagccatggc ctccatctgc tcgattacca gcttgatcgc
     3001 tccgggctgc ttgtcaggcg ggtacttgtg cttgacgagc agccgcttga tggacgatcg
     3061 gagctttgca cggacgtcgt cgcgaaccat ccagtccgtc ttgacgtccc ggcgcatcac
     3121 ctcgacgagc tcgcgggcta tctgcgcgag gacgtcctcc ccttggagcg cgaccgccga
     3181 ctcgttcgtg ctgacggcgt cgtagaaggc gagttcgtcc tccgacaggg gcggggtgaa
     3241 ctgccggccg cggttggctt cggccaccac ctccttcgcc agctcgatca gctccgcaat
     3301 aacctccgcg gaggtgagct gctggttggt gtacttgttc atcagctcct tgatcctctc
     3361 ggagaacgcc cgttgccgta cgaggttgtg ccgggccact ctgccggatt cctcggtgag
     3421 caggtcgcgc agcgcctcga tcgccagatg cgggttctcc gccttctcgg ccttgagctg
     3481 gaactccggc ccgaggtcgg acagcgacgg cttgggcagc ccagcggcct ggtagatatc
     3541 gacgatctca ccggtcgcgg tcgacgaggc gaccagcccg gacagcaggc gctggatctc
     3601 ctcgggtacg ggctcgcccg acgcctgccg ctcctgggcg tcgaacttcc ccatccacac
     3661 gcggacctct tcgtagaatc gtgcgatggg gcggaggtct tcaagggtct cgcggcctcc
     3721 cgccagtgcc caggctcgag cgagctggtt cgcgagcttg cggaatcgtt gagccactgt
     3781 ctcctcgccg tcggcgacct ggtttcccgg tgtcgacggt gagcgcaggt ggttcgtgag
     3841 cccgacagca gccttgatcc agcttctcgt atcgccgagc agctgcttgc gccagggata
     3901 gccggagcag atctgatcga gcgcctcgat cagcgaccgt gcgagcaccg ctgcctcgtc
     3961 gacgttgcga ccgacgggct gcttggtgcg gtcagtcttc gtgtactccg ccagcgcctt
     4021 gttgaggttg tccgccagcg gtgcgtacgc caccagcagc ccgctcggct tgccgcggaa
     4081 ggtgcggttg acccgggcca gcgtctgcat gagcagcgct cccttgagcg gccggtcgac
     4141 gtagagcgtg tgcagcggcg gcgcgtcgaa gccggtgagc atcatgtcct tgacgatgac
     4201 gatctgcagc ggatcctcgg cgtccttgag gcgcttctgg atcaccttgt tcagggcatc
     4261 ccgacgtacg tgcttggcga cgagaccggt gtccccggcc gagccggagt agacgacctt
     4321 gatcacgccc ttgtccacgg cgtcgtcgtg ccacgcaggc cgcagcctga cgagctcctc
     4381 gtagagattg gcgcagatct ccctcgtggc gccgacgatg aacgccttgc cgggcgagga
     4441 gatgaagggg cgcatggcct ctgaccgctg gtcccagtgg tggaggatgt cggtcgccag
     4501 ggcggcgagt cgggcgggtg cgccgtacac ggcattgatc actgcgaccg acttctcgat
     4561 ccggtcgcgc tctacatcgt cgagcccgac ggttgcctca tccgcggctt cgtccagctg
     4621 ctcctcggtg acctcgccgg cgagcttgac cgagatgagc cggggctcga agtagaccgg
     4681 cacggtggcg ccgtcctcga cggcccgggt gaggtcgtag gtgtcgatga cgtccccgaa
     4741 cacctcttgc gtgttgcggt cggcgaagga gatcggcgtg ccggtgaagg cgatgaacgc
     4801 cgcgttgggc agcgcatcgc ggatgtgccg ggcgaagccg tcgaggtcgt cgtagtggct
     4861 gcggtgcgcc tcgtcgacga tcacgatgat gttccgtcgg tcggtcagca gcggatggtc
     4921 gaggccggcg tggcgttcgt cgtccgtgag cccgaacttc tgcagcgtgg tgaagtagat
     4981 gccgccggtg gtgcggtggg tcagctcgtc ccgcagctgc tcgcgcgtcg tgaccttcac
     5041 cgggctctcg gcgagcagct gcgagcggtt gaatgtctcg aagagctggc tgtcgagctc
     5101 cgtacggtcg gtgacgacga cgagcgtcgg gttcttcagc ttgggctgca ggctgaccag
     5161 gtgggcgtag agctccatct ccatcgactt gcccgagccc tgggtgtgcc agatgacgcc
     5221 ggccttgccg ttgctctcga ccgcgtcgac ggtcgacccg acggccttgg tcacggcgaa
     5281 gtactggtgc ggcttggcaa tccgcttcgc gtagccctcc gcgcccgcgt cgaatgcgac
     5341 gaagttccgc tgcagctgga ggaaccgttc ctggttgcag acaccgtcga tgaggtattc
     5401 cagctcgacg ccgaggctca tgtcgctgac gtcagtgact ggcgtgcgcg gtcggccgtc
     5461 gtcgtcgacg ttccagggcg agtagtggtt gagcggcgtg aacggcgtgc cgtaccgcgc
     5521 ggtgatgccg tcactgatga tcgacagcat gcagaagcga aaggccatcg ggaactcgcg
     5581 gatgtacgtc gccagctgcg cgtgcgcaga ggcgaggtca gcgcgctcgg caccggcctt
     5641 cttcagctcg acgatcacga ccggcatgcc gttgagatag aggacgatgt cgaaccgccg
     5701 ctcgacctcc gacgaccgca ccgtcacctg gttcaccgcg aggaactcgt tgtcttcgac
     5761 caggtgacta accagccgga tcgtcggcgt ctgctcgatg ccgtcggcgt cgacatagct
     5821 gatcccgcgg tagccgtcga ccaggatctg atgcagccgg aagttctcag cgatggcgtc
     5881 ctgcgacgtc ggcgccacga tctcagccag cgcctgctcg atgtactgcc cgggcaccat
     5941 cgggttaagc cggcgcatcg acgctaggag ccgcccacga aggacgatgt cctcccagga
     6001 cgtgcggcca ccctcaacac cgggcgcgac ctggttcccg tgcagcgtct cccacccgtg
     6061 ctcgaagagc cggtcgagcg cgatctcctc ccactcagcc tcgctgacca tcagacaacg
     6121 ccccccacaa ccttctccgc gtccctcaca cgaaccctcc ccgacatcaa caacggcagc
     6181 agttcatccc gggtggaagc cagtcgtccg ttctccttca acagcacggc cgccctggcg
     6241 tgcaaagggc ccacagtacg gtcgaattcg cgcgccatct cgaccgcagg caggcggacc
     6301 tccaatgact tgaatcgccc acgaggaagc tctaggaacg tcgccccatt tgcgtaggac
     6361 aagaagtctt gaacgcggaa gcgcatctcg tgaaagagcc accattgata aaccggctcc
     6421 ttggcgttga caacaatgaa cccctggttc actgccgtcg ccacctccgc gaccgcaaat
     6481 gcgccgatcg tcgctcgtga agtcataaga atcgagccag ccggataaac acttgacgcg
     6541 caagctgcca atctggccgc cgtaatcctt ctggccgtgc cgctcaggta cggagccgcc
     6601 agtgcagtga cgtctgtcgg agtagcccac gggatgtcgc ccccccagta ctcgtcaaca
     6661 gatgtccgcg gtgtcccccc tccacccacg tctgcaacgt ctccgaatgt cagtgcggcc
     6721 tcggagagca cgccggcggc ccggacgaag atcgagtcgg caaggttctg cgatagttcg
     6781 agttggcgaa cgttggcggc
//
LOCUS       DASVOR010001233         1871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_538715,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001233 DASVOR010000000
VERSION     DASVOR010001233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538715"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF516_23450"
     CDS             complement(<1..325)
                     /locus_tag="VF516_23450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110715.1"
                     /translation="MTKGSDMERQLTFIDVDENRLELLLKPDRVQLVVVDSRPELEAA
                     ELRRFASVLFAEYVARDLADRLAAARSRLQEARDRAGGGDPASGELVAALDGVLSAID
                     DLARSD"
     gene            577..1020
                     /locus_tag="VF516_23455"
     CDS             577..1020
                     /locus_tag="VF516_23455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110716.1"
                     /translation="MHIFTARVHDGRLVILDPDPASLGLKEGDSIDLVAVDELLLQGG
                     ALSEDDERAALHRAIEVSLAEEDAGMVVELDEVLRLEGQRAAAERAAGGRREVRASSD
                     GGPWTPPAREGTTVPRCSTACGHVQASLADVRRFGTFTVTRLPPS"
     gene            1591..>1871
                     /locus_tag="VF516_23460"
     CDS             1591..>1871
                     /locus_tag="VF516_23460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110717.1"
                     /translation="MKRCHGGHNEFAFRVMEQQKGNRHSQRERKRPLLRQDPVHGRCG
                     SHAKIEVYHQATRNQDTAIGHFTAALDVISYLLPSLQLEAPAACHPDRRR"
BASE COUNT          325 a          644 c          588 g          314 t
ORIGIN      
        1 gatccgagcg agccagatcg tcgatcgcgc tcagtacacc gtcgagcgcc gcgaccagct
       61 cgccggacgc cgggtccccg ccgcccgcac gatcgcgggc ctcctgcagc cggctgcgcg
      121 ccgccgccag ccggtccgcg agatcacgcg cgacgtactc ggcaaacaac accgacgcga
      181 accggcgcag ctcagccgcc tccagctccg gccggctgtc gaccaccacg agttgcacgc
      241 gatccggttt cagcaacagc tccaggcggt tctcgtcgac gtcgatgaac gttagctggc
      301 gttccatatc agaccccttc gtcaagttcg cgggccggtc ccgcgcgccc tacacaccac
      361 aacgacggcg aggcgctcgt cccgccgcct ctgatcgccg atgctgtccg gcatccgggc
      421 gagagcgcga aaaagagcat cacgccgccg gcgaaaccga agccgccata cgagagccaa
      481 acgagaaagc gtcgcgccgc ttgtccgaat cgatgctggc gcgcacggtg agagagattg
      541 cagatgcgct gatgagcacc ggttaggctt gcggtcgtgc atatcttcac cgcgcgcgtt
      601 catgatggtc ggctggtcat ccttgatccg gacccggcat ccttggggct gaaagagggg
      661 gacagcatcg acctggtagc ggtcgacgaa ctcttgctcc aagggggcgc gttgtccgag
      721 gacgatgaga gagccgctct gcatcgagcg atcgaggtgt cgctggcaga ggaagatgcg
      781 ggcatggtgg tcgagctcga cgaggtgcta agattagagg gacagagggc ggcggctgaa
      841 cgcgcggcgg gcggacgacg cgaagtccgg gcgagctcgg acggcggccc gtggactccg
      901 ccagcgaggg aaggtacgac ggtcccacgt tgctcgacgg cttgcgggca tgtgcaggca
      961 tcccttgccg acgttcgtcg gttcgggacg ttcacggtca cgcgactgcc cccgtcgtga
     1021 atggagatcg ggacctcggc aggatcgtta gattggacca tcgccacctc gagcatctgc
     1081 atgccacttc cctttcgcat ccatgcagta cgtttgacaa gcattgcttg tcggttctgc
     1141 acgccttctc ctgctcgctc tcggtgcgaa cgcgtacggt tcggtggact tctgcggctc
     1201 gtcggacacg gagtccccca caatgtggtg ctacttatcc tttgcgattc cctgcgatcg
     1261 aggtcgtgct cgcatgcttt catcgtcgtg tcggacctcg ccgcgcgtcc cgcgtcagcc
     1321 agggcctcga tcgctgccgc attgtcgccg ccagccccga acaccggtgt gctcctggtc
     1381 gagctcggcc tcgccgagcg ccccgcgcgg gccaggacct cgagctcgcc gatcaccgcc
     1441 ctgctgtcgc cgccgccgag ctcgccgtcg ccgccccgct ttcgctgtcg tcgccgccga
     1501 tgtcgccggc gctgcaatcg ccctcgagac ctggctgcga tgggtggctt atcgatgaaa
     1561 cgacgagccc cgggagccga tacaccagag atgaagcgct gccacggcgg tcataacgaa
     1621 ttcgccttca gggtcatgga gcagcagaaa ggcaaccgcc acagccaacg agaacgcaag
     1681 cgcccccttc tgcgacagga cccagtccat gggcgatgcg gttcgcacgc gaaaatcgag
     1741 gtctaccacc aagcgacgcg caaccaagac acggcgatcg gccactttac ggccgctctc
     1801 gacgtcatca gctacctgtt acccagtttg caactcgagg cccccgccgc ttgccatccc
     1861 gatcgccgcc g
//
LOCUS       DASVOR010001234         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_392072,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001234 DASVOR010000000
VERSION     DASVOR010001234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392072"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VF516_23465"
     CDS             complement(<1..1116)
                     /locus_tag="VF516_23465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEX8110718.1"
                     /translation="MHAGRQANCCANAHGLRGTGDARLEGMENESSGPPGLREHLARL
                     YPGATIVAIEPLGPDAGATAGRKSGDASTTKAAGYGLPVRIELRDGDATRQLVWRVAS
                     ANEFGHDRRADRAAVMVQAFEDFARIPQHVQAVDLGVIRGGELVSTRDATEHYLITSY
                     APGTIYAHDLRRIAAERIATPRDVDRVDALARYLAALHTPIAPPGTRYRRAIRDLVGS
                     GEGIFGIIDGYPDDVPGAPAARLAAIEARCAEWRWRLRGRDARLTRTHGDFHPFNIVF
                     DGDVPALLDASRGGCGDPADDVTALAINFVLFALDAPGAWRHGLGVLWRHLWPRYLAE
                     RPDDELLEVAPPFFAWRALVVANPVFYPRMTAAARDKL"
     gene            1128..1565
                     /locus_tag="VF516_23470"
     CDS             1128..1565
                     /locus_tag="VF516_23470"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8110719.1"
                     /translation="MIPTNILVPLDFSACSEHALDYACDLAARLGARIHVINAIGATL
                     PELSVALTAQMISSLRASNTAALDKLLEPRRKKVAFGDVTVADGDARDAILHAARAVR
                     ADLIVIGTHGRRGLTRVLLGSVAEDILRRAPCPVLAVRLETQP"
     gene            1562..1990
                     /locus_tag="VF516_23475"
     CDS             1562..1990
                     /locus_tag="VF516_23475"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8110720.1"
                     /translation="MKRLLVALDGSPRAQTVLAAAARLAELAGSKLILFRAISVPVDM
                     PREILSLLDRRLEDVLIGNAHADLERFAAGLPRDRVERIVTPFATAWDGICRTARELD
                     VDLIVIGSHGYGGIDRVLGTTAAKVVNHADRNVLVVRTLL"
     gene            complement(2004..>2358)
                     /locus_tag="VF516_23480"
     CDS             complement(2004..>2358)
                     /locus_tag="VF516_23480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110721.1"
                     /translation="IASAPAPGCQGSADDLEAIAVGDAQLGGPVLALAATGGGHRESS
                     TWLGLYRVMNGAVAPIFTGMVEERTGDRTRTGEVTLLPGALLYRAPSGEQTLWVYRPE
                     QRRYVEQALVLPPSA"
BASE COUNT          299 a          970 c          782 g          307 t
ORIGIN      
        1 cagcttgtcg cgcgccgccg cggtcatccg gggatagaac accgggttgg cgaccaccag
       61 cgcgcgccac gcgaagaacg gcggcgcgac ctcgagcagc tcgtcgtcgg gccgctccgc
      121 caggtagcgc ggccacaggt ggcgccacag caccccgagc ccgtggcgcc aggcgcccgg
      181 cgcgtcgagc gcgaacagca cgaagttgat cgccagcgcg gtcacgtcat cggccggatc
      241 gccgcagccg ccgcggctgg cgtcgagcag cgccggcaca tcgccgtcga acacgatgtt
      301 gaacgggtgg aagtcgccgt gcgtccgggt cagccgcgcg tcgcgcccgc gcagccgcca
      361 ccgccactcg gcgcagcgcg cctcgatcgc cgccaggcgg gccgccggcg cgccgggcac
      421 gtcgtccgga tagccgtcga tgatcccgaa gatcccctcg ccgctgccca ccaggtcgcg
      481 gatcgcgcgg cggtagcgcg tcccgggcgg cgcgatcggt gtgtgcaggg ccgccaggta
      541 gcgcgccagc gcgtcgaccc ggtcgacgtc gcgcggcgtc gcgatccgct cggccgcgat
      601 ccggcgcaga tcgtgcgcgt agatcgtccc cggtgcgtag ctcgtgatca ggtagtgctc
      661 ggtcgcgtcg cgcgtcgata ccagctcgcc gccgcggatc accccgaggt cgaccgcctg
      721 cacgtgctgc gggatccgcg cgaaatcctc gaacgcctgc accatcaccg ccgcgcggtc
      781 cgcccgccgg tcgtggccga actcgttggc cgacgcgacg cgccacacca gctgccgcgt
      841 ggcgtcgccg tcgcgcagct cgatccgcac cggcagcccg tagccggcgg ccttggtcgt
      901 cgacgcatcc ccgctcttgc ggcccgccgt cgcgcccgcg tcggggccga gcggctcgat
      961 cgccacgatg gtcgcacccg ggtacagccg cgccaggtgc tcgcgcagcc ccggtgggcc
     1021 cgaagactcg ttctccatcc cctccagccg tgcatcgccc gtgccgcgca agccgtgcgc
     1081 gttcgcgcag cagttggcct ggcgcccggc atgcacggct gtttcgcatg atcccgacga
     1141 acatcctcgt ccccctcgac ttcagtgcgt gctcggagca cgccctcgac tacgcgtgcg
     1201 acctcgcggc caggctcggc gcccggatcc acgtgatcaa cgcgatcggc gccacgctgc
     1261 cggagctctc ggtcgccctc accgcccaga tgatctcgtc gctccgcgcc agcaacaccg
     1321 ccgccctcga caagctgctc gagccgcggc gcaagaaggt cgcgttcggc gacgtcaccg
     1381 tcgccgacgg cgacgcccgc gacgcgatcc tccacgccgc ccgcgcggtc cgcgccgacc
     1441 tgatcgtcat cggcacccac ggccgccgcg gcctgacccg cgtcctgctc ggcagcgtcg
     1501 ccgaggacat cctgcgccgc gcgccgtgcc cggtgctcgc cgtccgcctg gagacccagc
     1561 catgaagcgc ctgctcgtcg ccctcgatgg atcgccgcgc gcccagaccg tgctcgccgc
     1621 cgccgcccgg ctggccgagc tcgccggcag caagctcatc ctgttccgcg cgatcagcgt
     1681 cccggtcgac atgccgcgcg agatcctgag cctcttggac cgccgcctcg aggacgtcct
     1741 gatcggcaac gcccacgccg acctggagcg gttcgccgcc ggcctgccgc gcgaccgcgt
     1801 cgagcgcatc gtcacgccgt tcgccaccgc gtgggacggc atctgccgca ccgcccgcga
     1861 gctcgacgtc gacctgatcg tcatcggctc gcacggctac ggcggcatcg atcgcgtgct
     1921 cggcaccacc gccgccaagg tcgtcaacca cgccgatcgc aacgtcctgg tcgtccgcac
     1981 cctgctctag ctcgggctac gggctacgcg ctcggcggca gcaccagcgc ctgctcgacg
     2041 tagcggcgct gctccggccg gtacacccac agcgtctgct ccccggacgg cgcgcggtac
     2101 agcagcgcgc ccggcagcag cgtcacctcg ccggtgcgcg tccggtcccc ggtgcgttcc
     2161 tcgaccatcc cggtgaagat cggcgccacc gcgccgttca tcacgcggta cagcccgagc
     2221 caggtcgacg actcgcggtg cccgccgccg gtcgccgcca gcgcgagcac cggcccaccg
     2281 agctgcgcgt cgcccaccgc gatcgcctcg aggtcgtccg ccgagccctg gcagcccggt
     2341 gccggcgccg acgcgatc
//
LOCUS       DASVOR010001235         6191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363172,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001235 DASVOR010000000
VERSION     DASVOR010001235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363172"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1746)
                     /gene="purL"
                     /locus_tag="VF516_23485"
                     /gene_synonym="purI"
     CDS             complement(<1..1746)
                     /gene="purL"
                     /locus_tag="VF516_23485"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF003672.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEX8110722.1"
                     /translation="MLIVPGRAALSAARQARALAKGRAVCPGLRGLTARWLHLVVTTR
                     PLTAAEATQLDAMLAYGPRGEETPVSPAGSVETLAFYVAPRIGTTSPWSSKATDIAHV
                     CGLAAIARIERCIAYAATGIGLSARALGAALSDRMTESVIVREADLSQVVAHGGAPRP
                     LGTIALGSRLGSPGTAPAALRAASDRLGLALADDEIAYLIERYRELGRDPTDVELMMF
                     AQANSEHCRHKIFNAEFHLAGERQPQSLFQLIKRSTERAPDGVLSAYRDNAAVVEGVV
                     AERLFPGPDGAYRAHREPSHILGKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGA
                     KPKAGLVGFTVSDLRLPGALEPWEPLAERWIGSPARIATALEIMTDGPLGGAAFNNEF
                     GRPAVLGYFRTFEMAEPAGAGGRVARGYHKPVMLAGGIGAIRPGHIAKAPVPAGAALI
                     VLGGPAFLIGLGGGAASSLAQGASAEDLDFASVQRDNPEIQRRCQEVIDRCWGMGDAN
                     PILSIHDVGAGGLSNALPELVHDAGLGAAIDLRAIPTGEPDLSPLELWCNEAQERYVL
                     AIAPERVAEFAALCTR"
     gene            2119..2991
                     /locus_tag="VF516_23490"
     CDS             2119..2991
                     /locus_tag="VF516_23490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110723.1"
                     /translation="MPELFEEEHTEVLSRAVWTHRRPSMRWLGGAAMWFTAAIAITLC
                     APHSTHTLAPATVVALEHQAGELGLTIDAAVRAAHQRAQSVANTPMMRAAILTDAVTV
                     ADVMKSELKFQLAPGEVVELFQIHDGQTETLIRMPAAAPALPGVGDRDVTRVEIDGTG
                     LRVIASERVERLKNGEGYDSRTTGMFMLSSPVGLEAIRQQIAAYAEDATLVDASRAIH
                     LVHQASATTGHTLSLPVPTEAAKLTLTVVPELTGHRAPWLDLARNISVVLGALMLIMF
                     ALMLALHHRKLRRG"
     gene            3321..4022
                     /locus_tag="VF516_23495"
     CDS             3321..4022
                     /locus_tag="VF516_23495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110724.1"
                     /translation="MLAAVLAFAACNAPLPGMPPSPDNGGAVDAPLPADASNVVRPDA
                     HAADARSADANPVPADAAGPDAGRTGGGDAGSSGGDAGSSGSDGGSAGSDAGGSGGGG
                     GGNGVISCYTEGNPGATCTLPTHCCFSRYSSQHDGACATSACDYGTIECDGPEDCASG
                     QHCCAHAIVDPQAGLIGYQLACQASACGAAPANQEMCHPTTASAAGTCSSPTSRCVTA
                     FGNDNDLPPSLHICQ"
     gene            4377..5003
                     /locus_tag="VF516_23500"
     CDS             4377..5003
                     /locus_tag="VF516_23500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110725.1"
                     /translation="MMFDQRLRSSHALAVALALGACTAPSIGDPTSSAADAGVEIDIV
                     GRVRRPPPPPPDAPTYTPDASVPTGGGGGGGGDPSLPGVISCYTEGNSGATCNLPTQC
                     CFGNYSSAHNGSCSTQSCAWGTESCDGPEDCPSGQHCCAHVLIDDYWGIYGYQLSCQA
                     SACGAAPANQEMCHPTATSAAGTCSTGTSCVTALGNDADLPPNLHICK"
     gene            complement(5034..6047)
                     /locus_tag="VF516_23505"
     CDS             complement(5034..6047)
                     /locus_tag="VF516_23505"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEX8110726.1"
                     /translation="MSGPSGSIDDAVLRAQLGRTLDATPFRTIRGQALERYDGKVRDC
                     FIDRGRGERIIIVTDRLSAFDAVVGTIPFKGQVLNQLAQFWFEHTRAIAPNHMLAVPD
                     PNVMIARECEPLPVELVMRAYLTGVTSTSIWKAYEAGARTFCGHRLADGMRKNQPLPR
                     PILTPSTKAAKGDHDVSVSRDELLAMGRISPELFDRAAELAAALFAAGQRHAASRGLI
                     LADTKYEIGIDRSRPAGDSLVIIDEIHTPDSSRYWYADDYEARLARGEEPRSLDKEYV
                     RRWLAGDARWSGDGPPPVMPDDIRVEAARRYIASYELVTGRSFVPDTGDPVPRIAAAL
                     DAA"
     gene            complement(6044..6172)
                     /locus_tag="VF516_23510"
     CDS             complement(6044..6172)
                     /locus_tag="VF516_23510"
                     /inference="COORDINATES: protein motif:HMM:NF021883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110727.1"
                     /translation="MLGADPEIAARLDAAELDALFDLEHHLRHAPEILERALGSLT"
BASE COUNT          903 a         2246 c         2192 g          850 t
ORIGIN      
        1 gcgggtgcac agcgccgcga actccgcgac gcgctcgggc gcgatggcga gcacgtagcg
       61 ctcctgggcc tcgttgcacc acagctccag cggcgacaga tcgggctcgc cggtcgggat
      121 cgcgcgcagg tcgatcgccg cgccgaggcc ggcgtcgtgc accagctcgg ggagcgcgtt
      181 cgacagccca ccggcgccga cgtcatggat cgagaggatc gggttggcgt cgcccatccc
      241 ccagcagcga tcgatcacct cctggcagcg gcgctggatc tcggggttgt cgcgctggac
      301 cgacgcgaag tcgaggtcct cggccgaggc accctgggcc agcgacgacg cggcgccgcc
      361 gcccaggccg atcaggaacg ccggcccgcc gagcacgatc agcgcggcgc ccgccggcac
      421 cggcgccttg gcgatgtggc cggggcggat cgcgccgatc ccgccggcga gcatcaccgg
      481 cttgtggtaa ccgcgcgcca cgcgtccgcc cgcgccggcg ggttccgcca tctcgaacgt
      541 gcgaaagtag ccgagcacgg cgggccggcc gaactcgttg ttgaacgcgg cgccgccgag
      601 cggcccgtcg gtcatgatct ccagcgccgt tgcgatccgg gccggcgagc cgatccagcg
      661 ctctgccaga ggctcccacg gctccagcgc gccgggcagc cgcaggtccg acaccgtgaa
      721 gccgaccagg ccggccttgg gcttggcgcc gcgcccggtc gcgccctcgt cgcggatctc
      781 gccgcccgag ccggtcgccg cccccggaaa cggcgagatc gccgtggggt ggttatgggt
      841 ctcgaccttg ccgaggatgt gcgacggctc gcgatgagcg cgatacgcgc cgtccggccc
      901 ggggaacagc cgctcggcga ccacgccctc gaccaccgcg gcgttatcgc ggtacgccga
      961 cagcacgcca tcgggcgcgc gctcggtcga ccgcttgatc agctgaaaca gcgactgtgg
     1021 ctgccgctcg ccggcgagat ggaactcggc gttgaagatc ttgtggcggc agtgctcgct
     1081 gttggcctgc gcgaacatca tcagctcgac gtcggtcgga tcgcggccga gctcgcggta
     1141 gcgctcgatc aggtacgcga tctcgtcgtc ggcgagcgcc agccccagcc gatcgctcgc
     1201 ggcgcgcagc gcggccggag ctgtgcccgg cgagccgagc cgggatccga gagcgatcgt
     1261 cccgagcggc cgcggcgccc cgccgtgcgc cacgacctgg gacagatcgg cctcgcggac
     1321 gatcacgctc tcggtcatgc gatcggacag cgccgctccg agcgcgcgcg ccgacagccc
     1381 gatcccggtc gccgcgtacg cgatgcagcg ctcgatccgc gcgatcgctg cgagcccgca
     1441 cacgtgggcg atgtcggtcg ccttcgacga ccacggcgag gtggtgccga tccgcggcgc
     1501 gacgtagaac gccagggtct cgaccgagcc cgccgggctg acgggcgttt cctcgccgcg
     1561 cggcccgtag gcgagcatcg catcgagctg ggtcgcctcc gccgcggtga gcggccgggt
     1621 ggtgaccacg aggtggagcc agcgcgccgt cagcccgcgc aacccggggc acaccgcccg
     1681 tcccttcgcc agtgcacgcg cctgccgggc cgcgctgagc gccgcgcggc cggggacgat
     1741 cagcacgacg caccgggacg aggcgagaca gcgagtcgca tctcccacag cagacaccac
     1801 gaatgatgcg tccgtgcaag ccccccgtga gactgggcga tccgaggctg ggcgatcccg
     1861 atgaggaggg ctcgctcgac ccggctgcgc cgtctccacg tgggggtcgg agaatggagg
     1921 atcggcgaac cggccgcgcc aaccgcgggc ggtaccgcgc tcccaggtag ggccatggcc
     1981 ggatcgcaag cgggtgactg aatgcagcga gctcggcagt cgattgaccc actcgccggt
     2041 gctccggatc gcggcgattg acggtcaccg cggcggcgga ttacgctggt ggcactacga
     2101 gggcctacag gtaagcgtat gccggagctg ttcgaagaag aacataccga ggttctctcg
     2161 cgcgccgtgt ggacgcaccg ccggccgagc atgcggtggc tcggcggtgc cgcgatgtgg
     2221 ttcacggccg ccattgcgat caccctgtgt gcgccgcact cgacccacac gctggcgccc
     2281 gcgacggtcg tcgcgctgga gcaccaggcc ggcgagctcg gcctgaccat cgacgccgcc
     2341 gtccgcgcgg cccaccagcg cgcgcagagc gtggcgaaca cgccgatgat gcgcgccgcg
     2401 atcctcaccg acgcggtgac cgtcgccgac gtgatgaaga gcgagctcaa gttccagctc
     2461 gctcccggcg aggtggtcga gctgttccag atccacgacg gccagaccga gacgctgatc
     2521 cggatgccgg ccgccgctcc cgcgctgccc ggggtcgggg atcgcgacgt cacgagggtc
     2581 gagatcgacg gcaccggcct gcgggtcatc gcgagcgagc gcgtcgagcg gctcaagaac
     2641 ggcgagggct acgactcccg gacgaccggc atgttcatgc tgtcgagccc ggtcggcctc
     2701 gaggcgatcc gccagcagat cgcggcgtac gccgaggacg ccacgctggt agacgccagc
     2761 cgcgcgatcc acctcgtcca tcaggcatcg gcgaccaccg gccacacgct gtcgctcccg
     2821 gtgccgaccg aggccgcgaa gctgacgctc accgtcgtgc ccgagctcac cggccaccgc
     2881 gcgccgtggc tcgatctcgc gcgcaacatc tcggtcgtgc tcggcgcgct gatgctgatc
     2941 atgttcgcgc tgatgctcgc gctgcaccac cgcaagctcc ggcgcggtta gctcgccgcg
     3001 cgcccgtcgc gttcgcggaa cacaccgcgg gctgtcaagc ggcaaacttc agcgcacgcg
     3061 gacgcacgat gtgaccacgc tgcgctcgac cgcggacatg gcgctacccg cgcgctgtcc
     3121 ggttcacggc gcacaggtgc gcagccgtag ggatgatggc gtcggcggtg gctgcattct
     3181 gcatcgtgag gcgtgcgcga tcgccacgtg tgaaatgcta gcgtctgcca gctgccgcaa
     3241 atgtcgtgag agaaagtaac tcctcacggc aaacgtgtgc ggcccccacg atgccctccc
     3301 cgcgattccg atcctcgtgc gtgctggcgg ccgtgctggc gttcgctgcg tgcaacgcac
     3361 cgttgccggg catgccgccg tcgcccgaca acggcggcgc agtcgacgct ccgctgcccg
     3421 ccgacgcgag caacgtggtg cgacccgacg cgcacgccgc cgatgcgcgc agcgccgacg
     3481 cgaacccggt cccggccgac gccgccggcc ccgacgccgg caggaccgga ggcggtgatg
     3541 ccggcagctc gggcggcgat gctggcagct cgggcagcga tggcggcagc gcgggcagcg
     3601 acgccggtgg atctggcggc ggtggcggcg gtaacggcgt catcagctgc tacaccgagg
     3661 gcaaccccgg cgcgacgtgc acgctgccga cccattgctg cttcagtcga tacagctcgc
     3721 agcacgacgg cgcgtgcgcg acgtcggcgt gcgactacgg aaccatcgag tgcgatggcc
     3781 ccgaggactg tgcgagcggc cagcattgct gtgcgcacgc gatcgtggat ccccaggccg
     3841 ggctgatcgg ctaccagctc gcgtgccagg cctcggcgtg cggcgccgct ccggccaacc
     3901 aggagatgtg ccacccgacg accgcgtcgg cggccggcac gtgctccagc ccgaccagcc
     3961 ggtgcgtgac ggcgttcggc aacgacaacg atctcccccc gtcgctgcac atctgccagt
     4021 agttgccggc ggcaccgcgc gcgatcgccc ggcgatcacc gccgccgttc gaccatctgt
     4081 ccggactccg gacagatggc tccagccgac cacaccggcc gcaccggccg cagatccggc
     4141 gcaccattcc cttcaattgc gctgtcagat agcagtcgcc gtcgttcata gatcgccgga
     4201 tcttcgtcga cgaggtgcag cgacctacac tgccggctat tccaaggtag gaatcacacg
     4261 cctgcaattc cgtaatgatc tttaatccga ttacgaagat ttctgggcat cgacgtgtat
     4321 tatcgaaact ggttccggtg tctcaaaatg cattctttac gagaggggtc ctccacgtga
     4381 tgtttgacca gcggctccga tcctcccatg ccctggccgt cgccttggcg ctcggcgcgt
     4441 gcaccgcgcc ctcgatcggc gacccgacca gctccgcggc cgacgcgggc gtcgagatcg
     4501 acatcgtcgg ccgggtccgg cgcccgcccc cgccacctcc ggacgctccc acgtacacgc
     4561 cggacgcgag cgtgccgacc ggcggcggcg gcggcggcgg cggcgatccg agcctgcccg
     4621 gcgtgatcag ctgctacacc gagggcaact ccggcgcgac ctgcaacctg ccgacccagt
     4681 gctgcttcgg caactacagc tcggcgcaca acggctcgtg ctcgacccag tcgtgcgcgt
     4741 gggggaccga gagctgcgac gggcccgagg actgcccgag cggccagcac tgctgcgccc
     4801 acgtgctgat cgatgactac tggggtatct acggctacca gctctcctgc caggcgtcgg
     4861 cgtgcggcgc cgccccggcc aaccaggaga tgtgccaccc gaccgcgacg tcggcggccg
     4921 gcacgtgctc gaccggcacg tcctgcgtca ccgcgctcgg caacgacgcc gacctgccgc
     4981 ccaacctgca catctgcaag tagcggtcac cgcggccgtc acgggcacaa cgcctaggca
     5041 gcgtcgagcg cggcggcgat ccgcgggacg ggatcgcccg tatcggggac gaagctgcgt
     5101 ccggtgacga gctcgtacga cgcgatgtag cgccgggcgg cctcgacccg gatgtcgtcg
     5161 ggcatgaccg gcggcgggcc gtcgcccgac caccgggcgt ctccggcgag ccagcgccgg
     5221 acgtactcct tgtcgaggct gcgcggctcc tcgccgcggg ccagccgggc ctcgtagtcg
     5281 tcggcgtacc agtagcgcga cgagtccggc gtgtggatct cgtcgatgat caccagcgag
     5341 tcgcccgccg gccggctgcg atcgatgccg atctcgtact tggtgtcggc gaggatcagg
     5401 ccgcggctgg cggcgtggcg ctggccggcg gcgaacagcg ccgcggcgag ctcggcggcg
     5461 cggtcgaaca gctccggcga gatccggccc atcgcgagca gctcgtcgcg cgacaccgag
     5521 acgtcgtgat cgcccttggc ggccttggtc gacggcgtca ggatcggccg cggcagcggc
     5581 tggttcttgc gcatcccgtc agccagccgg tgcccgcaga acgtgcgcgc gcccgcctcg
     5641 taggccttcc agatcgaggt cgaggtcacg ccggtgaggt acgcgcgcat gaccagctcg
     5701 accggcagcg gctcgcactc gcgcgcgatc atcacgttgg gatcgggcac ggcgagcatg
     5761 tggttgggcg cgatcgcccg ggtgtgctcg aaccagaact gcgcgagctg gttgagcacc
     5821 tggcccttga acgggatcgt gccgaccacg gcgtcgaacg cgctcagccg gtcggtcacg
     5881 atgatgatgc gctcgccgcg cccgcggtcg atgaagcagt cgcggacctt gccgtcgtag
     5941 cgctcgagcg cctggccgcg gatggtgcgg aacggcgtgg cgtcgagcgt gcggccgagc
     6001 tgggcgcgca ggacggcgtc gtcgatcgat ccgctcgggc cgctcacgtc aggctcccca
     6061 gcgcgcgctc gaggatctcg ggcgcgtggc gcaggtggtg ctcgaggtcg aacagcgcgt
     6121 cgagctcggc ggcgtcgagc cgcgccgcga tctccgggtc ggcgccgagc agcgcgcgga
     6181 accggcccgg c
//
LOCUS       DASVOR010001236         1723 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_274929,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001236 DASVOR010000000
VERSION     DASVOR010001236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1723
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274929"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VF516_23515"
     CDS             complement(<1..377)
                     /locus_tag="VF516_23515"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8110728.1"
                     /translation="MLTSSPSAIPAIPTLDPAATGTATGTATAAATRRRVDPRLILSL
                     AAVYVVWSSTYLAIRFAVAELPPLLMAGGRYSIAGLTMLAIARRRGAAWPSAAQWLRV
                     VPIGALLFLCGNGFVVIGELSVSS"
     gene            519..>1723
                     /locus_tag="VF516_23520"
     CDS             519..>1723
                     /locus_tag="VF516_23520"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8110729.1"
                     /translation="MATSSGFLYEHLAAELGQAIDRGALRAGDRLPSVRRFAQERSVS
                     VATVLEAYMRLENAGLIEVRPKSGHFVRRRSALTAEPRRPRSCSTPSRVTVSDAYMKI
                     LAAMRDPELLPLGCATVDQAFLPIQALNRLVIQVTREMTTVGARYEGAPGLVTLRRQI
                     ARRAVEAGVALGENDLCTTIGATEALSLALRAVAKPGDVVAVESPAYFGVLQTIEGLG
                     LRALEVPANPRTGLDVAVFEDLLKSQPVRALVVTPTVSNPLGSVMSDEDRERLVKITR
                     RYDVPIIEDDVYAELVFDGSRPRPLRAFAGPSEDSHVLLVGSVSKTLAPGYRVGWLAG
                     GRWHDQILRLKFSHTLACPTLPGMAVAEFLASGGYDRHLRRLRAALATNVERYRECIA
                     TEFPEGTRVS"
BASE COUNT          257 a          585 c          639 g          242 t
ORIGIN      
        1 gacgacaccg acagctcgcc gatcacgacg aagccgttgc cgcacaggaa cagcagcgcg
       61 ccgatcggca cgacgcgcag ccactgcgcc gcgctcggcc acgcggcgcc gcgccggcgc
      121 gcgatcgcga gcatcgtcag ccccgcgatc gagtaccgcc cgccggccat cagcagcggc
      181 ggcagctcgg cgaccgcgaa ccggatcgcg aggtaggtcg agctccagac cacgtacacc
      241 gcggcaagac tgaggatcag gcgcggatcg acccggcggc gggtcgcagc ggccgtcgca
      301 gtaccagtcg cagtaccggt cgcagccgga tccagggtgg gaatcgccgg aattgccgac
      361 ggagaggacg tcaacacgcg cttcatcttg aggcctcttg cccagcagca cagagacaca
      421 gaattacatc acgtaccggt acagcggggg ctgtgctggt cgggcggtcg gtaactgtca
      481 tggtcgggtt tgcacgcgac gagtacagta gtcgcgtggt ggcaacctct tcaggcttcc
      541 tgtacgagca tctggccgcc gagctgggcc aggcgatcga tcgcggagcg ctgcgggcgg
      601 gtgaccggct gccgtcggtg cggcggttcg cccaggagcg cagcgtgtcg gtcgcgacgg
      661 tgctcgaggc atacatgcgg ctcgagaacg ccgggttgat cgaggtccgg cccaagtcgg
      721 ggcacttcgt gcgccggcgc agcgcgctga ccgccgagcc gcgccggccg cgcagttgct
      781 cgacgccgtc gcgggtcacg gtgtcggacg catacatgaa gatcctcgcg gcgatgcgcg
      841 acccggagct gttgccgctg ggctgtgcga cggtcgatca ggcattcctg ccgattcaag
      901 cgctcaaccg cctggtgatt caagtgacgc gcgagatgac cacggtcggc gcccgctacg
      961 agggcgcgcc cgggctggtc acgctgcgcc gccagatcgc gcggcgcgcg gtcgaggccg
     1021 gggtcgcgct cggcgagaac gatctatgta cgacaatcgg cgccaccgag gcgctgtcgc
     1081 tcgcgctgcg cgcggtcgcc aagccgggcg acgtggtcgc ggtcgagtcg ccggcgtact
     1141 tcggcgtgct gcagacgatc gagggcctcg ggctgcgcgc gctggaggtc ccggccaacc
     1201 cgcggaccgg gctcgacgtc gcggtgttcg aggacctgct gaagagccag ccggtccgcg
     1261 cgctggtggt gacgccgacg gtgtccaacc cgctgggctc ggtgatgtcg gacgaggacc
     1321 gcgagcggct ggtcaagatc acgcggcgct acgacgtgcc gatcatcgag gacgacgtct
     1381 acgccgagct ggtgttcgac ggctcgcggc cgcggccgct gcgcgcgttc gccggcccga
     1441 gcgaggacag ccacgtgctg ctggtcggct cggtgtccaa gacgctggcg ccgggctacc
     1501 gggtcggctg gctggccggc gggcgctggc acgatcagat cctgcggctc aagttcagcc
     1561 acacgctggc gtgcccgacc ttgccgggga tggcggtcgc cgagttcctg gccagcggcg
     1621 gctacgatcg ccacctgcgg cggctgcgcg ccgcgctcgc gaccaacgtc gagcgctacc
     1681 gcgagtgcat cgccaccgag ttccccgagg gcacgcgggt gtc
//
LOCUS       DASVOR010001237         9753 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_217229,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001237 DASVOR010000000
VERSION     DASVOR010001237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9753
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217229"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..694
                     /locus_tag="VF516_23525"
     CDS             137..694
                     /locus_tag="VF516_23525"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8110730.1"
                     /translation="MQIRDLDPESPEEREVCAQLLVEEFRDIAPDAWPILEKARETVD
                     ECLRNGPVRVARVYDTIVGWIGGHHLFSRVWELHPLVVARMAQKQGVGRALVADLERI
                     AAARGAMTLILGSDDEVGLTSLAGIELYPNPLEHLSRLEDRRGHAFTFYLKCGFVVTG
                     VVPDANGFGQPDIHMAKRVGSRWTR"
     gene            complement(816..1913)
                     /locus_tag="VF516_23530"
     CDS             complement(816..1913)
                     /locus_tag="VF516_23530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110731.1"
                     /translation="MSEVGGEGIGPSWAEFRQNPPVTWEAFRDTAARESSSYRFVVDG
                     DIVLSDEQKLWDYYQEWLLQEYESRVSGGSALTVRNVMGADVLWSSSQKFNLTYCISN
                     SFGSNKNAVIAAMGTATHSWSDRVSVGFAYRPDQDAACTNTNTNVLFNVAPVSDSFFA
                     ASFFPDDVRANRQLLITSAAFTTTEGGRDFQGILRHETGHTLGFRHEHIWITCTGETT
                     ADARHVNSYDVNSVMHYPQCRPSGTGGYRQTSSDFSGAISLYGNAQPLGCVPDGGIDD
                     TLGVTSCCSGFAVNGSTVCSNPQDFGTTWASCNHICGTAPVNGCIPSGGWDDTLFSTS
                     CCSGQAVPGSTWCLDPSDYGTDWASCVQKCQ"
     gene            2545..2679
                     /locus_tag="VF516_23535"
     CDS             2545..2679
                     /locus_tag="VF516_23535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110732.1"
                     /translation="MSNNSALTSLDFTSLTRVGGDVHLEALPGVPPEQIAELLHRLGR
                     "
     gene            complement(2705..4045)
                     /locus_tag="VF516_23540"
     CDS             complement(2705..4045)
                     /locus_tag="VF516_23540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110733.1"
                     /translation="MKTRALHEEALATVERLQKIHPDLTEQFEKSYGYAVFPSVGRAS
                     ITVGVTFGRGIVFERKLPIGTATITELTLGVQVGGQTFSELMFFPSKHELENLKRGEL
                     AFAANASAVLVKAAASGTTDFRGVIAKAYSQGGMLLELSLGGQKLRFSPDANIGGRRE
                     EVPRSAERADMEAKAPPEMAREESEREDRWHEPPAAGVGGQEEARRAHEEEGEARERP
                     SAEHWNPGRRARSILRRVERALPMSIRARLAPVTGALGGRRLGKGLLAPVRRVLDRAI
                     VPLRNEQVVSKTVHPAAHAALVHLIERNETLRDRLDRAAGYAIFPSVGKATAVLGATY
                     GRGEVYERGRLIGYAALVQITIGVQLGGDTFIELVIFDKPDALKRFKEGKVAFAANAA
                     AVIVKAGVAATRSSPPGADVRVVTEGGLLLETALGGQKFVYRPAVLTRGKKAIN"
     gene            4290..4874
                     /locus_tag="VF516_23545"
     CDS             4290..4874
                     /locus_tag="VF516_23545"
                     /inference="COORDINATES: protein motif:HMM:NF016983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HEX8110734.1"
                     /translation="MARRHAANHAELSRDTRGPGEAATHVIRTCSIASAIAAMRPFPA
                     VDLARVTPIQVGMVRSIARIYGYPLVLRDARKLIGAIGMSVVVQDMVIAAVKLVPVAG
                     VPVAISGAYALTYAIGEAAKAYFEHGRTLSIRRLRAVFRRAYHRQRGAIGRAAAQNER
                     LITELRRLAKLYSAGQIGEAEYVRRKQELTSGLA"
     gene            complement(4991..5176)
                     /locus_tag="VF516_23550"
     CDS             complement(4991..5176)
                     /locus_tag="VF516_23550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110735.1"
                     /translation="MAAVRGPARRAPAAEHGGPSQFRIARLEPWTIPMIYWSAGAVVL
                     VAWGWSLWSVLEALFSG"
     gene            complement(5218..5538)
                     /locus_tag="VF516_23555"
     CDS             complement(5218..5538)
                     /locus_tag="VF516_23555"
                     /inference="COORDINATES: protein motif:HMM:NF018765.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1360 domain-containing protein"
                     /protein_id="HEX8110736.1"
                     /translation="MTVYALIIGCLAVWRVTHLVNAEDGPFDVVVRLRALAGAGMAGK
                     LMDCFYCLSLWVAIPFALLLEDTLKDRALLWLAISGGAGLLQRFSSPGMGAVDGAKTA
                     MRGE"
     gene            complement(5557..6705)
                     /locus_tag="VF516_23560"
     CDS             complement(5557..6705)
                     /locus_tag="VF516_23560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110737.1"
                     /translation="MSAPQYTKCVDRNDYADPNFGPEIFVAIAGLIYAISTFGISLVF
                     SMAAAMSALMKVCDYILNGKLVCLGDDQCAVGRVASFETVDDKSGVDKIDNDFSINLL
                     LAPHHLDEFAHGTQEANYKWLIKDTTSWGHDGLPGKLVTEQSGMPLPRESDGGSWHTA
                     RYSGTFKTYSNSNYLSWEDPPTPGTPFDVPVFHCEIEGERAHAVCSTLAALSSPIPGF
                     GAVCSFKLFGIPLGRWACAIIAAILAPFVLAALGIAWAAGSDDNRSFDGAGSLKRGDP
                     VIIRGRWCYDASHSGWNEIHPVMSVQRIENDQDLDGPMFKDAIAQWCSLTGEVPPVTD
                     PGVRPQGMTPEQSTVYDNQQRPENQWVIHPYVDGCEPVETPPPPPVIH"
     gene            complement(6769..7806)
                     /locus_tag="VF516_23565"
     CDS             complement(6769..7806)
                     /locus_tag="VF516_23565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110738.1"
                     /translation="MNLKVMIIQLCFVIFLFLIMMLLIRIRRRKRRARRPGMRNRKES
                     SRPCATVTPQVYRRPDPMIYDQYYLMKQGIAVTWDNPDIHLELGGVTVPSESLLPSTT
                     YDVVARIWNGSTDAPAVNMPVRFSYLTFGIGQKQVPIGETLVNLPVKGAPGCPSFARV
                     KWTTPSVAGHYCLQVELVWSDDANPGNNLGQENTNVKALNSPRAKFTFPVANDTGRDQ
                     ILVLEADTYAVPARRPCRDQELDRQPDANPERQKPRSPADEIARRQRDAQALHDRRAY
                     PVPTGWRVDIEPRELVLAPGAEQEVTVDITAIDGYVGRQVLNVHAFDVSVDRDHRRLV
                     GGVTLYVTGTG"
     gene            complement(7958..8530)
                     /locus_tag="VF516_23570"
     CDS             complement(7958..8530)
                     /locus_tag="VF516_23570"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HEX8110739.1"
                     /translation="MLVQLTSAYVAVLGAEVVGDKTLYTVGSLAAHHRISAVVAGASA
                     AMAVKMTAAVYFGQFLARLPPFVLALASMVTFAGLAVSVWLKRQRVPAVPPPGRHASW
                     RSSSLAFAAIFCTEWADPGQMAAALLSARYRAPAVVLLGATLAAVSKVLVAATAGVSM
                     RRWVSDTALRFFTTIVCTTMCVLAALQVEL"
     gene            complement(8914..9264)
                     /locus_tag="VF516_23575"
     CDS             complement(8914..9264)
                     /locus_tag="VF516_23575"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEX8110740.1"
                     /translation="MTDRDHLLELGAAALAAGDWLAAHEHWEQAWRRTGPDDRHAVQA
                     LAQLAAALVHLSRGRPRGAARVLAKAQRKLARPATPATIGPIDVALVRRQSDRLSDDL
                     AAGRTPDLSAIRIA"
     gene            complement(9326..9637)
                     /locus_tag="VF516_23580"
     CDS             complement(9326..9637)
                     /locus_tag="VF516_23580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110741.1"
                     /translation="MVVRHRVLPIAEFRVHKGQNFGPLVMPLPGSKRSVIGFPQLTGT
                     NDVVGGSDPDEWTRYVSTVWQLDGDKVRQLPGAAARAVIQAAHKSATPPAQDPSSAPQ
                     P"
BASE COUNT         1645 a         3276 c         3117 g         1715 t
ORIGIN      
        1 ccgtcgccgt gccgccggtc gccgtcgacc gccgcgacga gctgtgacac cggcgcccgc
       61 gccagcgtcg agccgcgcga tgtgggagtc gctaggcttc tcttgttcga cggccacatg
      121 gacgctacga acaagcatgc agattcgcga cctcgaccct gagtcccccg aggagcgtga
      181 ggtttgcgcc cagctgctcg tcgaggagtt ccgcgacatc gcgccagacg catggcccat
      241 cctcgagaag gcgcgagaga cggtcgacga atgcctacgc aacggccctg tccgcgtcgc
      301 acgcgtctac gacacgatcg tcggctggat cggcggccat cacctgttct cgcgggtctg
      361 ggagctacac ccgctggtcg tcgctcgcat ggcgcagaag cagggcgtcg gacgtgccct
      421 cgtggcggac ctcgagcgga tcgcggcggc gcgcggcgca atgacgttga tcctcggcag
      481 cgatgacgag gtcggcctga cgtcgctcgc ggggatcgag ctctatccga atccgctcga
      541 gcatctctcg cggctcgaag atcgccgcgg gcacgcgttt accttctatc tgaagtgcgg
      601 gttcgtcgtg acgggtgtcg tgcccgatgc aaatgggttc ggtcagccgg atatccacat
      661 ggccaaacgc gtcggttcgc gatggacgcg ttgagtaccg cgtcgaggag ccgtgggttt
      721 ggaggcagtc gttgggggct tggcgccgcg cgcggccttc ctgtcgccca ggatggagat
      781 gaagtcgggg cgacgcgccg tgcttgcagc cgaggctact ggcatttttg gacgcaggac
      841 gcccagtcgg tgccatagtc actgggatcc aggcaccagg tgctccccgg cacagcctgg
      901 ccgctacagc aggaggtgct gaacagcgtg tcgtcccagc ctcccgaggg gatgcaacca
      961 ttcactggcg ctgtaccgca gatgtgatta cagctcgccc acgttgtccc gaaatcctgc
     1021 ggattggaac aaaccgtgct gccgttcacc gcgaaaccgc tacaacacga cgtcacgccg
     1081 agcgtgtcat cgatacctcc atcgggcacg caacccaggg gctgtgcatt gccatacagt
     1141 gagatcgccc ccgagaaatc ggagctggtc tgtcgatacc ccccagtccc gctcggcctg
     1201 cactgcggat agtgcatcac cgagttcaca tcgtagctgt tgacatgtct ggcgtccgcc
     1261 gtcgtttccc cggtgcaagt aatccagatg tgttcgtgcc ggaaccccag cgtgtgtccg
     1321 gtctcatgcc gcagaatgcc ctggaagtcc cgtccgccct cggttgtcgt gaacgcggcg
     1381 ctggtgatca ggagctgccg gttcgcccgg acatcgtctg gaaaaaacga cgcggcaaag
     1441 aagctgtcgg acaccggagc gacgttgaac aggacattgg tgttggtgtt ggtgcatgcc
     1501 gcgtcctggt cgggtctgta ggcgaagccc actgacacgc ggtcactcca cgagtgagtg
     1561 gcagtaccca tggcggcgat aaccgcgttt ttgttcgaac cgaacgaatt gctgatgcaa
     1621 tacgtcaggt taaacttctg cgaagaggac cacaggacat ccgcgcccat cacgtttctc
     1681 actgtaagcg cagagccgcc agacaccctg ctctcatact cttgcaggag ccactcctga
     1741 tagtagtccc agagcttctg ctcgtcggac agcacgatgt ctccgtcgac gacaaagcga
     1801 tatgatgagg actcccgggc tgcggtgtcg cgaaaagcct cccacgtcac gggagggttt
     1861 tggcgaaatt ccgcccagct cgggccgatg ccttccccgc cgacctcgga caagttgact
     1921 gcgtcctcgg ctgcacaccc caccgcacat gcacatatca acatccagca ttgtgacttc
     1981 atgacaacca tcctttgtcg ggattaccga tcacatcacc gcacgtggta gtcttccggg
     2041 cgtcaaaccg caatcagcga cccaaggaat cgcatcaagc caccatcccg gctttgcctg
     2101 tcacgagcgg tggaactccg gttgtaacgt cagcgatgac gtatcagact catcgtcttg
     2161 ggcgcacgag gcgccatcac tcttactcta gtctcactgg attttggcat ctgcgttgtg
     2221 cagcatagta ctgtggaccg gttgcaccgg cgtttggagg tcgaacagag gcacgatggg
     2281 ctgaggtggg gtgcggtgag tcactcggtg gaccggcagc ttgcgcaggg tagccagcgg
     2341 tgatccggta gcgcaccgcc gcgcggcgct gcagccgccg gcccccaggc ccgaccctcc
     2401 gctcaccggc tgccgctcac gcagagcggc catcgcaccc agagctcgat agcctgcaga
     2461 tccagcgtct cgctgtcctt caattccgcc ctcgtccaca cggcgggcct ggcgaacctg
     2521 accgcgatcg gcggctcgct cacggtgagc aacaactccg cgctgacatc gctcgacttc
     2581 accagcctca cgcgcgtcgg cggcgacgtg cacctcgagg cgctgcccgg cgttccgccc
     2641 gagcagatcg ccgagttgct gcaccggctc ggccgctgag ccggtgcacc gagcagttcg
     2701 agcttcagtt gattgccttc ttgccccgcg tcaggacagc aggccgatac acgaacttct
     2761 gtccgccgag cgctgtttcg agcagcaggc cgccctccgt cacgacccgg acgtcggcac
     2821 cgggcggact gctccgggtc gcggcgaccc cagccttgac gatgacggcc gcggcgttcg
     2881 cggcgaacgc gaccttgcct tccttgaacc gcttgagcgc gtcgggttta tcgaagatga
     2941 cgagctcgat gaaggtgtca cctccgagct ggaccccgat cgtgatctgg acgagggcgg
     3001 cgtatccgat caggcgacct ctctcgtaga cctccccgcg tccgtaggtc gcgccgagca
     3061 cggcggtcgc ctttccgacc gacgggaata tcgcgtatcc ggcggcccga tcgagacgat
     3121 cgcgcagcgt ctcgttccgc tcgatgagat ggacgagcgc ggcgtgcgcc gcgggatgca
     3181 ccgtcttgct cacgacctgc tcgttgcgaa gcggcacgat ggccctgtcg agcacgcggc
     3241 gcacgggagc cagcagcccc ttgccgagcc tcctgccgcc cagtgccccg gtgacgggtg
     3301 ccaggcgagc cctgatggac atcggcagcg cacgctcgac ccgccgcaag atcgagcgag
     3361 cgcgccgtcc gggattccag tgctcggcgc tgggtcgctc ccgggcctcg ccctcctcct
     3421 cgtgggctcg acgggcctct tcctggccac cgaccccggc agcgggcggt tcatgccatc
     3481 ggtcttcacg ctccgattcc tctcgggcca tctccggtgg cgcctttgcc tccatgtcgg
     3541 ctcgctcggc gcttcgcggc acctcctccc ttcgtcctcc gatgttcgcg tcggggctga
     3601 acctgagctt ctgtcctccg agggatagct cgagcagcat cccgccctgc gagtacgcct
     3661 tggcgatcac cccgcggaag tcggtcgtgc ccgaggccgc ggccttcacc aggacggcag
     3721 acgcattcgc tgcgaacgcg agctctccac gcttcaggtt ctccagctcg tgcttgctcg
     3781 ggaagaacat cagctcgctg aacgtctgtc cgcctacctg cacgccaagt gtgagctcgg
     3841 tgatcgttgc agtgccgatc ggcagcttgc gctcgaacac gattccccga ccgaaggtga
     3901 ctccgacggt gatagatgcg cggcccaccg acggaaacac cgcgtatccg taggacttct
     3961 cgaactgctc tgtcagatcc ggatggatct tctgaagtcg ctcgacggtg gccaatgctt
     4021 cttcgtgtag cgccctggtc ttcatgatcc gggatgcgtg caagatcgag ggtacgcagc
     4081 gcagccatct ccccccgtcg atccggctcg gaaccccagg acgatcgacc gagatctcgc
     4141 agggcacgcg tttcgcatta ccattcccgg cgacgaatgg gccgagtcca ccctgtactc
     4201 cgcagggcga tcgtgcggcc ggaacggcac gtcgcacgca gagcgaagtc atgaggcatg
     4261 agcaggttac gcacgcatgt gggtgtgccg tggctcgccg gcacgccgcg aatcacgccg
     4321 agctctcgcg cgacacgcgc ggtcccggcg aggccgccac gcacgtgatc cggacctgca
     4381 gtatcgcctc tgccattgcc gccatgcgtc cgtttccggc ggtcgatctc gcgcgagtca
     4441 cgccgatcca ggtcgggatg gtgcgctcga tcgcgaggat ctacgggtat cccctcgtac
     4501 tacgagacgc acggaagctg atcggcgcaa tcggaatgag cgtggtcgtg caggatatgg
     4561 tcatcgcagc cgtcaagctc gtcccggtgg cgggcgtgcc cgtcgcgatc tcgggcgcgt
     4621 acgcactcac ctacgcgatc ggcgaggccg ccaaggcgta cttcgagcac ggtcggacac
     4681 tctcgatccg ccgcctccgc gcggtatttc ggcgggctta ccaccgtcag cgcggcgcga
     4741 tcggacgcgc cgcagcccag aacgagcggc tgatcacgga gcttcgccgc ctcgccaagc
     4801 tctacagtgc cggacagatc ggcgaggccg aatatgtccg ccgaaaacag gagctcacct
     4861 ccggtctggc ctgacgctgc cgctaccggc gctcgagcac cagcgcgcga tcgccgcaag
     4921 cggtcgcccg ccgcccggtg atcgcgccga gatgcgcgtc gacgcagcgg tgcggacgcg
     4981 ccacatggca tcacccggag aacagcgctt cgaggaccga ccacagcgac cagccccatg
     5041 cgaccagcac gacagcgccc gccgaccagt agatcatggg gatggtccat ggttcgagcc
     5101 gtgcaatccg gaactgcgaa ggcccgccgt gctcggccgc cggagcccgg cgcgcgggtc
     5161 ctcgaaccgc cgccatccgg ctcgcctgga tccgtccttt tccacagcag gacatctcta
     5221 ttcccctctc atggcggtct tggccccatc caccgcgccc atccccggcg acgagaagcg
     5281 ctgcaggagc cccgcgccac ccgagatcgc cagccagagc agcgctcggt ccttgagcgt
     5341 gtcttcgagc agcagcgcaa acgggatcgc gacccacagg ctcaggcagt agaagcagtc
     5401 catcagctta ccggccatgc ctgcgcccgc cagggcgcgc agccggacga ccacatcgaa
     5461 cggtccgtcc tcggcgttga cgaggtgagt cacccgccac acggcgaggc aaccgatgat
     5521 gagtgcgtag accgtcacct cagcttccag cgcccatcaa tggatgacgg gcggtggcgg
     5581 cggggtctcc accggctcgc aaccgtcaac gtaggggtga atcacccact ggttctctgg
     5641 ccgctgttgg ttgtcgtaga cagtcgactg ctccggcgtc attccttgcg gtcgtacccc
     5701 ggggtccgtc accggaggga cttccccggt cagcgagcac cactgcgcga tggcatcttt
     5761 gaacatcggc ccgtccaggt cttgatcatt ttcaattctc tggacgctca tgacaggatg
     5821 gatctcgttc cagccggagt ggctggcatc gtagcaccag cgcccccgga tgatgaccgg
     5881 gtcgccgcgc ttcaggcttc cagcgccgtc gaagctgcgg ttgtcgtcgg aaccggcggc
     5941 ccaggcgatg ccgagcgccg cgagaacaaa cggcgcaagg atggcagcga tgatcgcgca
     6001 tgcccagcgg ccaagaggaa tgccgaagag cttgaagctg caaaccgcgc cgaagccggg
     6061 gatcgggctg ctgagcgcgg ccagcgtcga gcacaccgcg tgcgctcgct cgccttcgat
     6121 ctcgcaatga aagactggaa catcgaacgg cgtgcctggg gtgggtggat cttcccagct
     6181 gagatagttg ctgttcgaat aggtcttgaa cgttccggag taccttgcag tatgccagct
     6241 ccctccgtcg gactcgcgcg gaagcggcat gccactctgc tcggtcacca gcttcccggg
     6301 caagccatca tggccccagg acgtggtgtc cttgatcagc catttgtaat tcgcttcttg
     6361 cgtgccatgc gcgaactcat cgagatggtg cggcgccagc agcaggttga tcgagaagtc
     6421 attgtcgatc ttgtcaaccc cgctcttgtc gtcgaccgtc tcgaatgacg ctacgcggcc
     6481 gacggcgcac tggtcgtctc cgagacaaac aagcttgcca ttcaggatgt aatcgcacac
     6541 cttcatcagc gccgacatcg ccgccgccat cgagaagacg agcgagatgc cgaaagtgga
     6601 gatagcgtag atgagcccgg cgatcgccac gaagatttcg ggaccgaagt tgggatccgc
     6661 atagtcgttg cgatcgacac atttcgtata ttgaggagca ctcatggtcg attctttctt
     6721 gacacgggta ttggcattga ccgatcgact gcacgcgagt tccgctgact acccggtgcc
     6781 cgtcacgtag agcgtgacgc caccgacgag cctccgatgg tctcgatcca ccgacacgtc
     6841 gaaggcgtgc acgttcaata cctggcggcc gacgtatcca tcgatcgcag tgatgtcgac
     6901 cgtcacctcc tgctcggcgc ctggagccag gacgagctct cgcggctcga tgtccacccg
     6961 ccagccagtc gggaccgggt acgcgcggcg atcgtggagc gcctgcgcat cccgctggcg
     7021 gcgcgcgatc tcgtccgcgg gagaccgcgg cttctgccgt tcggggttgg cgtccggctg
     7081 ccggtcgagc tcctgatccc tgcatggacg gcgagcagga acggcgtagg tgtccgcctc
     7141 cagcacgagg atctggtccc gtccggtatc gttggctacc gggaacgtga acttcgcgcg
     7201 cggcgagttg agcgccttga cgttggtgtt ttcctggccg aggttgtttc cggggttggc
     7261 gtcgtcgctc cagaccagct ccacctgcag gcagtagtgt cccgccaccg acggcgtcgt
     7321 ccatttcacg cgagcaaagc tggggcaacc gggggcgccc ttcacgggca ggttgacgag
     7381 ggtctcgccg atgggcacct gcttctgacc gatcccgaag gtgagatagg agaaacggac
     7441 tggcatattg accgctggcg catccgttga tccgttccag attcgcgcta ccacgtcgta
     7501 ggtcgtcgat ggcaggagcg attcggaggg gacggtgacc ccgcccagct cgaggtggat
     7561 gtcggggttg tcccaggtga ccgcgatacc ctgcttcatc aagtagtact gatcatagat
     7621 catcgggtcc ggccgccggt atacctgcgg tgtgaccgtc gcacatggcc ggctcgactc
     7681 cttgcggttc cgcattcccg gccggcgtgc gcggcgcttc ctgcggcgaa tcctgatcag
     7741 cagcatcatg atcaagaaca ggaagattac gaagcaaagc tggatgatca ttactttcag
     7801 gttcattggc ctcccccttt gcactcgaga tctggctccg ccgtgctgga ctcgctgcca
     7861 gggatctcga tcagcggacc ggaccgagct ctgaccgcat ggcagcgacg gcctggaacc
     7921 gcggccgcgc gacccatctc actccgcgcg cgcggcttca cagttccacc tgaagggctg
     7981 cgagcacgca catggtcgtg catacgatgg tcgtgaagaa gcggagcgcc gtgtcagaca
     8041 cccatcgccg catcgagacg ccggcggtcg ccgccaccag gaccttgctc accgccgcga
     8101 gtgtcgcgcc caggagcacc acagccggcg ctcggtagcg cgcagagagc agcgccgcgg
     8161 ccatctggcc tggatctgcc cactcggtgc agaagatggc cgcgaatgcg agggaggatg
     8221 aacgccatga ggcgtggcgt ccaggcggcg gcaccgccgg tacgcgctgg cgcttgagcc
     8281 acacggagac ggcgagcccg gcgaaggtga ccatgctggc gagcgcgagc acgaacggcg
     8341 gaaggcgggc gaggaactgc ccgaagtaca cggccgcggt catcttcacg gccatggctg
     8401 ccgacgcccc cgcgaccacc gccgagatgc ggtggtgcgc ggcgaggctg ccgacggtat
     8461 agagcgtctt gtcaccgacg acctcggcgc cgagcacggc gacgtatgcc gaggtgagct
     8521 gcaccagcat cgtgacgttc ccccttcctc agctctccag atccggaaca catcgaaccc
     8581 ggcgtccaat cgcccggagc catgcgatga ggagtgtctg tacgctccgg gccagcacac
     8641 gacggttcac tccccgtgcc ctcatcgtgc gtcccccatg attctttttc gctctgtccc
     8701 tctcccttgt caatgtacaa tacaccggtt gccgtcctga tcaagcgatt gctgacgtgc
     8761 tcgcgagtgg ctcggatctg acgtcggtgg ttgctacgac cagcatggta atggttgcca
     8821 tcgcgatcgg atcgaagatc atcgaagcgc agcaagatga tgtgattgtc tgtcgtgcca
     8881 cacgtggttc gtcgacagca aagatcgcgc ggctcacgcg atccggatcg cgctcaggtc
     8941 aggagtccgg ccagcggcga gatcgtcgct cagccggtcg ctctggcggc gcaccagcgc
     9001 gacgtcgatc ggtccgatgg tggcaggtgt tgccggtcgc gccagcttgc gctgcgcctt
     9061 ggcgagcacg cgcgccgcgc cgcgcggccg gccgcgggac agatggacca aggcggcggc
     9121 gagctgcgcg agcgcctgga ccgcgtgtcg gtcgtcgggg ccggtccgcc gccatgcctg
     9181 ctcccagtgc tcgtgcgctg ccagccagtc accggcggcc agcgcggcgg ctccgagctc
     9241 gagcaggtga tcgcgatcgg tcacgggcgt gcggtccgtc ttcgcacatc gatctcgaag
     9301 ccgctgcggc ggggcacgcg gagcttcatg gctgcggcgc gctcgacggg tcctgggcag
     9361 gcggagttgc cgacttgtgg gcggcctgga tcaccgcgcg cgccgccgcg ccgggcagct
     9421 gccggacctt gtcgccgtcg agctgccaca ccgtggacac gtaccgggtc cactcgtccg
     9481 ggtcgctgcc gccgacgacg tcgttggtgc cggtgagctg cggaaacccg atgaccgatc
     9541 gcttgctgcc gggcagcggc atgaccagcg gcccgaagtt ctggcccttg tggaccctga
     9601 actcggcgat cggcagcacg cgatgccgca cgaccacggc gtgatgcccg tccggctggc
     9661 taccggcgcc cgagcaccag cgcgcgatcg ccgcagcgga cggcgcggcc gggcacgccg
     9721 cggcgcaccc gcccgctcgt gcgggtcaag aac
//
LOCUS       DASVOR010001238         1731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_13394,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001238 DASVOR010000000
VERSION     DASVOR010001238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1731
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13394"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..752)
                     /gene="ccsA"
                     /locus_tag="VF516_23585"
     CDS             complement(<1..752)
                     /gene="ccsA"
                     /locus_tag="VF516_23585"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX8110742.1"
                     /translation="MNPPTPFSPISLVGTIALLAAYAVAAWCVAAGIAGNARKSRRLV
                     NSSVYGLYGFAALITLASVLLIYAFVTHDFAIKYVAAVSDTTMETWYKVTSFWGGLDG
                     SLLLWVLVLALFSTVAIVVNHRRHREMIGYVVATIMVVQVFFLSLLIFTKNPFTPFLT
                     TPPPEGQGLNPLLQNYWMVIHPPSLYTGFVAATIPFAFGIGALASGRLDDLWLGSVRV
                     WMLICFGFLSLGLILGGRWAYEELGWGGYWAW"
     gene            complement(760..1248)
                     /locus_tag="VF516_23590"
     CDS             complement(760..1248)
                     /locus_tag="VF516_23590"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEX8110743.1"
                     /translation="MNRSTLGKIALTAVVAVGGIAFVLRSSASAERYFHVEELMAKEL
                     PEWTGKELKVHGIVEAGTIVEKIANQETQRTFVLQSKGKKIRVFSAGPKPDTFKDESE
                     VVATGHLVPAADLRPLAQQLGVAIESDMPYVVKASELMAKCPSKYGDKRIELQPTTPQ
                     YK"
     gene            complement(1525..>1731)
                     /locus_tag="VF516_23595"
     CDS             complement(1525..>1731)
                     /locus_tag="VF516_23595"
                     /inference="COORDINATES: protein motif:HMM:NF018201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110744.1"
                     /translation="LRLTATGALHACLGHDDAISLRDVIRGGGSDDDVVRAIAASVTG
                     KRAGHTFERTGGGAPHKHMIGIGG"
BASE COUNT          286 a          605 c          575 g          265 t
ORIGIN      
        1 tcccacgccc agtagccgcc ccagcccagc tcctcgtagg cccaccgccc gccgaggatc
       61 aggccgagcg acaggaagcc gaagcagatc agcatccaga cccggaccga gcccagccac
      121 aggtcgtcga gccggcccga cgccagcgcg ccgatgccga acgcgaacgg gatcgtcgcc
      181 gcgacgaagc cggtgtacag cgacggcgga tggatcacca tccagtagtt ctggagcagc
      241 gggttcaggc cctggccctc gggcggcggc gtggtcagga acggggtgaa cgggttcttg
      301 gtgaagatca ggagcgacag gaagaacacc tgcaccacca tgatcgtcgc gaccacgtag
      361 ccgatcatct cgcggtggcg ccggtggttg accacgatcg cgaccgtcga gaacagcgcc
      421 agcacgagca cccacagcag cagcgagccg tcgagcccgc cccagaacga ggtgaccttg
      481 taccaggtct ccatcgtcgt atcgctgacc gccgcgacgt acttgatcgc gaagtcgtgg
      541 gtgacgaacg cgtagatcag cagcacggat gccagcgtga tcagcgcggc gaagccgtac
      601 aggccgtaca ccgacgagtt gaccaggcgg cggctcttgc gggcgttgcc ggcgatgccg
      661 gcggcgacgc accaggcggc gaccgcgtag gcggccagca gggcgatcgt gccgaccagc
      721 gagatcggcg agaacggtgt gggggggttc accgtcggct cacttgtact gcggagttgt
      781 cggttgcagc tcgatgcgct tgtcgccgta cttcgacgga cacttcgcca tcagctccga
      841 cgccttgacg acgtacggca tgtcggactc gatcgcgacg ccgagctgct gggcgagcgg
      901 ccggaggtcg gcggccggga cgaggtgccc ggtggcgacc acctcggact cgtccttgaa
      961 cgtgtccggc ttgggccccg cgctgaacac ccggatcttc ttgcccttgc tctggagcac
     1021 gaacgtccgc tgcgtctcct ggttcgcgat cttctccacg atcgttccgg cctcgacgat
     1081 gccgtggacc ttgagctcct tgccggtcca ctccggcagc tccttggcca tcagctcctc
     1141 gacgtgaaag tagcgctccg cgctcgccga cgacctcagg acgaacgcga tcccgcccac
     1201 cgcgaccaca gcagtcagag cgattttacc cagggtgctt ctgttcatag gggttggcct
     1261 ccacagggcc acggcgtgcc cggcgactgg caaaccgtag catgatcaga accattcccg
     1321 cgcgcgccca cccgcggccg aaaacctggc ctgcgacgtt tttttgaggc cgcccgccga
     1381 ggctcctcaa caattcgcgc aggctgtgcg cgcaggtccg aggcgcgcgt cacgccaccg
     1441 tcgcgtcgag cgtacgtcgc gacaccgttt cgcaccgcgc cgatcgctga tggtcgctcg
     1501 gtcaataagg cgcgcgcgag gccgctagcc accgatgccg atcatgtgct tgtgcggtgc
     1561 gccgccgccg gtgcgctcga acgtgtgccc ggcccgcttg ccggtcaccg acgccgcgat
     1621 cgcgcgcacc acgtcgtcgt cgctgccgcc gccgcggatc acgtcgcgca ggctgatcgc
     1681 gtcgtcgtgg ccgaggcacg cgtgcagcgc gccggtcgcg gtgagccgga g
//
LOCUS       DASVOR010001239         1704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_538754,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001239 DASVOR010000000
VERSION     DASVOR010001239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538754"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="VF516_23600"
     CDS             <1..688
                     /locus_tag="VF516_23600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX8110745.1"
                     /translation="LDELGVPEPAAAAARAEAMRERGQTAMYVVVDGALAGLLGVADP
                     IKPSAADAIRALRADGLRIVMLTGDSRTTAAAVARELAIDPRDVIAEVLPADKAAAIT
                     RLRTGGEIVAMAGDGINDAPALAAAHVGIAMATGSDVAIESAGITLLGGDLRGLPRAR
                     RLSRGTMRNIRQNLVFAFAYNLLGVPIAAGMLYPVFGLLLSPMIASAAMSASSVSVIG
                     NALRLRRLPL"
     gene            complement(713..1156)
                     /locus_tag="VF516_23605"
     CDS             complement(713..1156)
                     /locus_tag="VF516_23605"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110746.1"
                     /translation="MATEDKVILLVDDDPNDVELTLRAFRASPLGNRIVVARDGLAAL
                     DYLCGTGAYAGRDPSHQPQVVLLDLNLPVIDGLEVLRRIRADERFRRLPIVILTSSNE
                     DSDKLAGYGLGANSYVRKPVDFRQFSEAVTRLGLYWMLVNEPPPR"
     gene            complement(1255..>1704)
                     /locus_tag="VF516_23610"
     CDS             complement(1255..>1704)
                     /locus_tag="VF516_23610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110747.1"
                     /translation="YSITNVDADGVVPRTSQWLRNWTQPGLNSFRANAPRDPAASRAE
                     PDLTVRQAKVTCGLAGPAVAAEVCNRGNQPVAAGLPVAVYATTTPSRQLRCQAQTAEP
                     VLPGSCTTVSCAWTGPYGDAAIVADNRGIVRECREDNNAMTLHVACR"
BASE COUNT          220 a          634 c          622 g          228 t
ORIGIN      
        1 gctcgacgag ctcggcgtcc ccgagccggc cgccgccgcc gcccgcgccg aggcgatgcg
       61 cgagcgcggc cagaccgcga tgtacgtcgt cgtggacggc gcgctcgccg gcctcctcgg
      121 cgtcgccgac ccgatcaagc cgtcggccgc ggacgcgatc cgcgcgctgc gcgccgatgg
      181 cctgcgcatc gtcatgctga ccggcgacag ccgcaccacc gccgccgccg tcgcccgcga
      241 gctcgccatc gacccgcgcg acgtgatcgc cgaggtcctc cccgccgaca aggccgccgc
      301 gatcacccgg ctgcgcaccg gcggcgagat cgtcgcgatg gccggcgacg gcatcaacga
      361 cgcccccgcc ctcgccgccg cccacgtcgg gatcgcgatg gccaccggca gcgacgtcgc
      421 gatcgagagc gccggcatca ccctgctcgg tggcgacctc cgcggcctgc cccgcgcgcg
      481 ccgcctgtcg cgcggcacga tgcgcaacat ccgccagaac ctcgtgttcg cgttcgccta
      541 caacctgctc ggcgtgccga tcgccgccgg catgctctac cccgtgttcg gcctgctgct
      601 gagcccgatg atcgccagcg ccgcgatgag cgccagctcg gtctcggtga tcggcaacgc
      661 cctccgcctg cgccgcctgc cgctgtagcc gcggcgacgc tgtgccggag cgtcagcgcg
      721 gcggcggctc gttgaccagc atccagtaca ggccgagccg ggtcaccgcc tcggagaact
      781 ggcggaagtc gaccggcttg cggacgtagc tgttggcgcc gaggccgtag ccggcgagct
      841 tgtcgctgtc ctcgttggac gaggtcagga tcacgatcgg cagccggcga aaccgctcgt
      901 cggcgcggat ccgccgcagg acctcgaggc cgtcgatcac cggcaggttg agatcgagca
      961 gcacgacctg cggctggtgg gatggatcgc ggccggcgta cgcgccggtg ccgcacaggt
     1021 agtcgagcgc cgccaggccg tcgcgcgcga cgacgatccg gttgccgagc gggctggcgc
     1081 gaaacgcgcg cagcgtcagc tcgacgtcgt tcgggtcgtc atcgacgagc aggatgacct
     1141 tgtcctccgt cgccacgacc tcggtactct atgtcagcgg ccggaggcag accagcgcga
     1201 tcgcgatgcc gcccgaccgg ccgcgacgcg gcgcgacatc gcgcgagacc gtcgtcagcg
     1261 acacgccacg tgaagcgtca tcgcgttgtt gtcctcgcgg cactcgcgga cgatgccgcg
     1321 gttatcggcg acgatcgcgg cgtcgccgta cgggcccgtc caggcgcagc tgacggtggt
     1381 gcagctgccg ggcaggacag gctcggcggt ctgcgcctgg cagcgcagct gccgggacgg
     1441 cgtggtcgtg gcgtagaccg cgaccggcag gccggcggcg accggctggt tgccgcggtt
     1501 gcagacctcg gcagcgacgg cggggccggc cagaccgcac gtgaccttgg cctgccggac
     1561 ggtgagatcg ggctcggcgc gcgacgccgc cgggtcgcgg ggggcgttgg cgcggaagct
     1621 gttgagcccg ggctgggtcc agttgcgcag ccactggctg gtccgcggca ccacgccgtc
     1681 cgcgtcgacg ttggtgatgc tgta
//
LOCUS       DASVOR010001240         2731 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421140,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001240 DASVOR010000000
VERSION     DASVOR010001240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421140"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF516_23615"
     CDS             complement(<1..197)
                     /locus_tag="VF516_23615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110748.1"
                     /translation="MIISYWLIRTPGCGAALPAWTPVVPEVSNPALSNESHADCRTDG
                     ALELVRGRGRACIVLAAAGGTV"
     gene            190..>2731
                     /locus_tag="VF516_23620"
     CDS             190..>2731
                     /locus_tag="VF516_23620"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8110749.1"
                     /translation="MIKMIGEGGMGSVFLARDLRLGRRVAIKFLQSNQPELTQRFLVE
                     ARTTARCQHDNIVVIYEVGEHNGAPYIVLEFLSGKPLTHLTENGQKLPYTRAVEIMCP
                     VLRALQCAHEHGIVHRDLKPDNIFITEAGTIKVLDFGIAKVMQQSHQDASAEKVGQPR
                     MPSPLELATGTNTSLTRVGTIMGTLKYMSPEQWGIGVEIDHLTDVWACGILLHRMICG
                     RHPLHPLDGNQLVVTAMLELPMPSMAEAVPTDVPRELIQIVDRCLLKNKEQRWQSAAE
                     LLSALEMFLPGRRTGTIKIDQSPYVGLSSFQEGNADTFFGRNREIAAMVTRIRDRPLM
                     AVVGSSGVGKSSFVRAGLVPALKRSGETWETLVIRPGRKPLEALAATIAPMVATAANL
                     ADDLEEQKKLVDTLGREPGHLGHVLRLRARRDNRHLLVFVDQFEELYTLCPDPAERAA
                     FTACLSAVADDATSPLRVVLSIRSDFLDRVAEDQQFVSEVSQGLFFLGPPSREGLRDA
                     ITQPAEMAGFRFERADVVEDMLNHLETTPGALPLLQFTAARLWETRDVARRMLTYQSY
                     AAMGGVAGALASHADRVVADIGPQKTPLVRALLLRLVTAERTRAIVPVAELRELSREV
                     GEVQRLIDQMVDARLLVVQNLEGGKGSTVEIVHESLVSGWPTLRHWLDENQDDAALVD
                     QLRVAARQWQAKKFDVGLLWRGDSAAEARKFRARYKGPLSDVERGFLDAVIHYDAAQA
                     RRRRTAIIAGFTTLSAVVVAMMVLLVVVQKSRTAAKDKEAEAIAQKQIAENRLSEKEK
                     ADRAREVAQAAQKVAEQEKQVITEQKAVVDVELNKSKEDLIKERDTANA"
BASE COUNT          437 a         1009 c          931 g          354 t
ORIGIN      
        1 accgtgccgc cggcggcggc cagcacgatg cacgcccggc cccgaccgcg gaccaactcg
       61 agcgcaccgt cggtgcggca gtcggcgtgg gactcgttcg acagcgccgg gttcgacacc
      121 tcgggcacga ccggggtcca cgccggcagc gccgcgccgc acccgggcgt ccggatcaac
      181 cagtacgaga tgatcaagat gatcggcgag ggcgggatgg ggagcgtgtt cctcgcccgc
      241 gatctccggc tcggccgccg cgtcgcgatc aagttcctgc agtccaacca gcccgagctg
      301 acccagcggt tcctggtcga ggcgcgcacc accgcgcgct gccagcacga caacatcgtc
      361 gtgatctacg aggtcggcga gcacaacggc gcgccgtaca tcgtgctcga gttcctgagc
      421 ggcaagccgc tgacccacct gaccgagaac ggccagaagc tgccgtacac ccgcgccgtc
      481 gagatcatgt gcccggtgct gcgcgcgctg cagtgcgccc acgagcacgg catcgtccac
      541 cgcgacctca agcccgacaa catcttcatc accgaggccg gcacgatcaa ggtgctcgac
      601 ttcggcatcg cgaaggtgat gcagcagtcg caccaggacg cgagcgccga gaaggtcggc
      661 cagccgcgga tgccgagccc gctcgagctc gcgaccggca ccaacaccag cctgacccgg
      721 gtcggcacga tcatgggcac gctgaagtac atgtcgcccg agcagtgggg gatcggcgtc
      781 gagatcgacc acctcaccga cgtctgggcg tgcggcatcc tcctgcaccg catgatctgc
      841 ggccgccacc cgctgcaccc gctcgacggc aaccagctgg tggtcaccgc gatgctcgag
      901 ctgccgatgc cgtcgatggc cgaggccgtg ccgaccgacg tgccgcgcga gctgatccag
      961 atcgtcgacc gctgcctgct caagaacaag gagcagcgct ggcagtcggc cgccgagctg
     1021 ttgtcggcgc tcgagatgtt cctgcccggc cgccgcaccg ggacgatcaa gatcgaccag
     1081 agcccgtacg tcgggctgtc gtcgttccag gagggcaacg ccgacacgtt cttcggccgc
     1141 aaccgcgaga tcgcggcgat ggtgacccgg atccgcgacc ggccgctgat ggccgtggtc
     1201 ggcagctcgg gcgtcggcaa gtcgtcgttc gtccgcgccg gcctggtccc cgcgctcaag
     1261 cgctccggcg agacctggga gaccctggtg atccggcccg gccgcaagcc gctcgaggcg
     1321 ctcgccgcca cgatcgcgcc gatggtcgcg acggcagcca acctcgccga cgacctcgag
     1381 gagcagaaga agctggtcga cacgctcggc cgcgagcccg gacacctcgg ccacgtgctc
     1441 cggctgcgcg cccggcgcga caaccgccac ctgctggtgt tcgtcgacca gttcgaggag
     1501 ctctacacgc tgtgccccga tcccgccgag cgcgcggcgt tcaccgcgtg cctgtcggcg
     1561 gtggccgacg acgcgaccag cccgctgcgc gtcgtgctgt cgatccgcag cgacttcctc
     1621 gaccgcgtcg ccgaggacca gcagttcgtc agcgaggtgt cgcagggcct gttcttcctc
     1681 ggcccgccca gccgcgaggg cctgcgcgac gcgatcaccc agcccgccga gatggccggg
     1741 ttccggttcg agcgggccga cgtcgtcgag gacatgctca accacctcga gaccaccccc
     1801 ggcgcgctgc cgctgctcca gttcaccgcc gcccggctgt gggagacccg cgacgtcgcg
     1861 cgccggatgc tgacctacca gagctacgcc gcgatgggcg gggtcgccgg cgcgctcgcc
     1921 agccacgccg accgcgtggt cgccgacatc ggcccgcaga agaccccgct ggtccgcgcg
     1981 ctgctgctcc ggctggtcac cgccgagcgc acccgcgcga tcgtcccggt cgccgagctc
     2041 cgcgagctgt cgcgcgaggt cggcgaggtc cagcggctga tcgaccagat ggtcgacgcc
     2101 cgcctgctcg tcgtccagaa cctcgagggc ggcaagggct cgaccgtcga gatcgtgcac
     2161 gagtcgctgg tctccggctg gccgacgctg cggcactggc tcgacgagaa ccaggacgac
     2221 gccgcgctgg tcgaccagct gcgggtcgcc gcccgccagt ggcaggccaa gaagttcgac
     2281 gtcggcctgc tgtggcgcgg cgactcggcg gccgaggccc gcaagttccg cgcccgctac
     2341 aagggcccgc tgtccgacgt cgagcgcggc ttcctcgacg cggtcatcca ctacgacgcc
     2401 gcgcaggctc gccgccggcg caccgcgatc atcgccgggt tcaccacgct gtccgcggtg
     2461 gtggtcgcga tgatggtcct gctcgtcgtg gtccagaagt cgcgcaccgc ggccaaggac
     2521 aaggaggccg aggcgatcgc ccagaagcag atcgccgaga accggctctc cgagaaggag
     2581 aaggccgacc gggcccgcga ggtcgcgcag gccgcgcaga aggtcgccga gcaggagaag
     2641 caggtgatca ccgagcagaa ggcggtggtc gatgtcgagc tcaacaagtc caaggaggac
     2701 ctgatcaagg agcgcgacac cgccaacgcc a
//
LOCUS       DASVOR010001241         7774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_275028,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001241 DASVOR010000000
VERSION     DASVOR010001241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7774
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275028"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..277
                     /locus_tag="VF516_23625"
     CDS             <1..277
                     /locus_tag="VF516_23625"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8110750.1"
                     /translation="TALATATAPATAAAMIDLVAVAAAWRGRGAGARLLAGCIDWLGD
                     WIGDRPGDHAHVATVGTQADNPALRLYRRCGFVPTATHVTYHLWLDG"
     gene            274..891
                     /locus_tag="VF516_23630"
     CDS             274..891
                     /locus_tag="VF516_23630"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8110751.1"
                     /translation="MTLGYAIKRALDYGLAAGGLLACAPAMAVIAAAIALDSPGGVFY
                     RQDRLGRGGRTFRLLKFRTMRDAPIRYNPDGSTRIDPHDDRVTRVGRWLRGGADELPQ
                     LVNVLRGEMSLVGPRPDMASQRALYTGDDERKLAALPGLTSLAVILGRNDIPWKQRVA
                     MDIYYVERWSLWLDLRILVMTLAMPLGLRGFDFSDVLGVQDTRDA"
     gene            895..2010
                     /locus_tag="VF516_23635"
     CDS             895..2010
                     /locus_tag="VF516_23635"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8110752.1"
                     /translation="MLFNIQVLRAIAALLVIHGHASGPSGLGLAWTDGANGVDLFFVI
                     SGFIIAYVASLDASQFMTRRLVRIVPIYWTSTLALYLLVLAMPRVFHTTSSDPVLLVR
                     SLAFLPTASSVHSNDGIPHPTLSGGWTLNYEMYFYAVFAIALAWSRPRATWLAVALLL
                     ALLAAIQLTPLRESPVAGFYGNPVILEFMYGIAAFHVVRWVEARRRVVPAPRGEPAAL
                     VAGVVLGLAVLVFNYELFSMAPRWLTYGILRGARWTTSGLPAFVVVVCAVLLERVHQI
                     RITNRWVILTGDASYVMYLIHAYVVFGVLRLVLGDRQLAELPGQLVVLGLMAVSTGAA
                     IAIYRWYEQPILRALKRRFIAPRRAAIRAAAPGASAR"
     gene            complement(1973..3094)
                     /locus_tag="VF516_23640"
     CDS             complement(1973..3094)
                     /locus_tag="VF516_23640"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8110753.1"
                     /translation="MKLAMVTTYATPTNMRRELLEAVLARGHRVTVVSPEPAGVMAGP
                     LAALGASYRAWSVNRTGIDPIEDLRSARHLHSILRAERPDVVLVYQIKAVLIAPLAAK
                     LARVERVVPLVNGLGAVFDSHGFGATARARLARRAYGMSLRLVDEIVFQNADDPALLQ
                     SLGLLSRRARWRVVPGTGVDLARLQRRPPHAGPPTFTLISRLLVSKGVRDLIAAARIV
                     RARHPQARFRIVGQLEADGHPDGVRRAEIDAWVAEGLIEYAGFSDDVAGVLAATTVFV
                     LPSYYREGVPRTNLEALAVGRPIVTTDWVGCRDTVEDGVNGFLIPPKDPVALADRLDR
                     YLRDPALVERHGAASRALAERRFDVRKVNAQMLQALQLG"
     gene            3232..4356
                     /locus_tag="VF516_23645"
     CDS             3232..4356
                     /locus_tag="VF516_23645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110754.1"
                     /translation="MRRPALVTWTIYLVLFPLYIFRSGLPQPGDLLVIVLVPLALQRW
                     NGRLGRDMRATMRALIWFTLWVCLVDYTWVLVMGSFTLFGPDSFLLFPLYYLYNSLIF
                     LVALVLYRRYGELFLRVTVFTVTAMVFIQVLSSVVLGSSSARGSVFFNNPNQLGYYAL
                     LAATLIALTYRRLQLRLWTASAALVCCGFLALISASRASAGGVAVLLVLMLFSNPKIL
                     VAACLAGAAMVSGVGPFADAIEASQDRVLNLSSHSDASFFQERGYDRIWRNQQYLLLG
                     AGEGGLSRFDDTAYVKNMEIHSSAGTILFSYGIVGSILFLVFLWRLLRGARARLAITL
                     LPPLLYTGAHQGLRFTMLWAVLAVFVALKDDVDRPRLEAA"
     gene            4490..6571
                     /locus_tag="VF516_23650"
     CDS             4490..6571
                     /locus_tag="VF516_23650"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX8110755.1"
                     /translation="MRSAVVVSALSGLSACAGLDLSSTEQNIDAPANVTATPLTDTQI
                     SVSWTAVPGALAYFVLDKKSTDTTFAFVGTTLDPGTSQLVIGLTPSTSYDFEVITEST
                     AITFSTPSAPVSATTFAVAPGAPTNVVATAVSNSEIDLSWTNFPGATAVYVLESQAGG
                     PNNFLTTVLAPGDTFAATGLIANTMYCYQLVSHLADGTLSGLSTPPACATTGAGPQPP
                     TGLIATAISDTRILVQWQAVADAAKYFVRESQSGGPFTLVGVNLAGDQSFTAVNLQPS
                     TSYCFTVSTVVADGTESAPSSLACATTLGVNNNGGFEGYWKLDERTGATALDSSGWNR
                     HGTITNAAYSLGDRAQLDDNRSAISFTASPDSAITVAPAPAFDLLDVFTVAFWAKLPA
                     TADVTFIGMRGAGCGALGWEIAQDASGLHFAGQTQTVAAGASVPVGVWTHVAVTYASG
                     TMHTFVNGVETASGAFTPDNTLQAPLNIGHVAGCAGGAVLMDDVLILGRAMSGTEVSA
                     IGTLPPPPTNVHVTSKTSASLVFAWDPVPGAVQYIISKGTAPGNETFFTHSPADSPSF
                     GDGNLTPNTQYSYNVRAVVGNLFSNPSNEVVDTTKPPPAPPTNVTATVSPPDRIQVQW
                     TAAPDAFVYFVFMSTAGGPSTFAGSVVAPATTFEAVNLSPATTYTFQVETEDSVLTVG
                     PKSQPASATTP"
     gene            6568..7554
                     /locus_tag="VF516_23655"
     CDS             6568..7554
                     /locus_tag="VF516_23655"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HEX8110756.1"
                     /translation="MKHWGVSFASAPSIASGPFKWSLTSTEPDRWRFRLHRSIIDATS
                     ALRVRGGLTLSRWLHRMVGDYTEPLTAEAGRDIRLSFSGFDRYWMRVFFRGDFYEPEL
                     YHWFRRVSRLRDFAFIDGGANIGFWSALLTAAEFGITRAVAIEASPTTYADLSRTAAL
                     CGQRFATEHRALTATSTPVIFEQGVGHASRHIVASDTPRTSASRHVVVEGTTVDALVA
                     RYGFDPRFLIVKLDVEGAEFASITGGSSAFSNGAIFIYEDHGKDPRSVLTEQLLDFGA
                     ACWFIADDGSMIPVDSATAASQFKLDHRRGYNYLCTSRTYGAKHRLEDRLFG"
     gene            complement(7560..>7774)
                     /locus_tag="VF516_23660"
     CDS             complement(7560..>7774)
                     /locus_tag="VF516_23660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110757.1"
                     /translation="PGVAGLILPGVNGWLAPVDDVAGLADALALATRTRAQLTGGAIR
                     DSVRTRFETGRIARRYEQAIAELARS"
BASE COUNT         1088 a         2789 c         2628 g         1269 t
ORIGIN      
        1 cacggccctg gccacggcca cggccccggc cacggctgcg gcgatgatcg acctcgtggc
       61 ggtggcggcg gcgtggcgcg ggcggggcgc cggcgcgagg ctgctcgccg gatgtatcga
      121 ctggctcggc gactggatcg gcgatcgccc gggcgatcac gcccacgtcg cgaccgtcgg
      181 gacccaggcc gacaaccccg cgctccggct gtaccggcgg tgcggcttcg tgcccaccgc
      241 gacgcatgtt acctatcacc tgtggctcga cggatgacgc tcggctacgc gatcaagcgc
      301 gcgctcgact acggcctcgc cgccggcggc ctgctcgcgt gcgcgccggc gatggcggtg
      361 atcgccgccg cgatcgcact cgacagcccg ggcggcgtgt tctaccgcca ggaccggctc
      421 ggccgcggcg ggcggacgtt ccggctgctc aagttccgca cgatgcgcga cgcgccgatc
      481 cgctacaacc ccgacggctc gacccggatc gatccgcacg acgaccgggt gacccgggtc
      541 ggccggtggc tgcgcggcgg cgccgacgag ctgccccagc tggtcaacgt gctgcgcggc
      601 gagatgagcc tggtcggtcc gcgcccggac atggcgtcgc agcgcgcgct gtacaccggc
      661 gacgacgagc gcaagctcgc cgcgctgccc gggctgacca gcctcgcggt gatcctcggc
      721 cgcaacgaca tcccgtggaa gcagcgcgtg gcgatggaca tctactacgt cgagcgctgg
      781 agcctgtggc tcgatctccg gatcctcgtg atgaccctgg cgatgccgct cgggctccgg
      841 ggattcgact tctccgacgt gctcggtgtc caggacaccc gggacgcata gccgatgctg
      901 ttcaacatcc aggtgctccg tgcgatcgct gccctgctgg tgatccacgg ccacgcgtcg
      961 ggaccgagcg gcctgggcct ggcgtggacc gacggggcca acggggtcga tctgttcttc
     1021 gtgatcagcg gcttcatcat cgcgtacgtc gcatcgctcg atgcgagcca gttcatgacc
     1081 cgccggctgg tccgcatcgt gccgatctac tggacgtcga cgctcgcgct gtacctgctg
     1141 gtgctggcga tgccgcgcgt gttccacacc acgtcgtcgg atccggtgct gctggtccgc
     1201 tcgctggcgt tcctgccgac cgcgtcgagc gtccactcca atgatggcat cccgcatccg
     1261 acgctgagcg gcggctggac gctcaactac gagatgtact tctacgcggt gttcgcgatc
     1321 gcgctcgcgt ggtcgcggcc gcgggcgacc tggctcgccg tcgcgctcct gctcgcgttg
     1381 ctcgccgcga tccagctcac cccgttgcgc gaatccccgg tcgccggctt ctacgggaac
     1441 ccggtcatcc tggagttcat gtacgggatc gcagcgttcc acgtcgtgcg gtgggtcgag
     1501 gcgcgccggc gcgtggtccc ggccccgcgc ggcgaaccgg cggccctggt cgccggcgtc
     1561 gtgctgggtc tggccgtgct cgtgttcaac tacgagctgt tcagcatggc cccgcgctgg
     1621 ctgacctacg ggatcctgcg gggagcccgc tggacgacca gcgggctccc ggcgttcgtg
     1681 gtcgtggtgt gcgcggtcct gctcgagcgt gtccaccaga tccggatcac caaccgctgg
     1741 gtgatcctca ccggagacgc gtcgtacgtg atgtacctga tccacgccta tgtcgtgttc
     1801 ggcgtgctcc ggctcgtgct cggcgaccgc cagctggccg agctccccgg gcagctggtg
     1861 gtgctcggcc tgatggcggt gtcgaccggc gcggcgatcg cgatctaccg ctggtacgag
     1921 cagccgatcc tgcgcgcgct caagcggcgg ttcatcgcgc cgcgccgcgc ggctatccga
     1981 gctgcagcgc ctggagcatc tgcgcgttga ccttccgcac gtcgaacctc cgctcggcga
     2041 gcgcgcggct cgcggcccca tggcgctcga ccagcgcggg atcgcgcagg tagcgatcga
     2101 ggcggtcggc gagcgcgacc gggtccttcg gcgggatcag gaagccgttg acgccgtcct
     2161 cgacggtgtc gcggcagccg acccagtcgg tggtcacgat cgggcggccc accgcgagcg
     2221 cctccaggtt ggtgcgcggc accccctcgc gatagtagga cggcagcacg aacaccgtgg
     2281 tcgccgccag caccccggcg acgtcgtcgg agaacccggc gtactcgatc aggccctccg
     2341 cgacccaggc gtcgatctcg gcgcggcgca cgccgtcggg gtggccgtcc gcttcgagct
     2401 gcccgacgat ccggaaccgc gcctggggat ggcgggcgcg cacgatccgg gcggcggcga
     2461 tcaggtcgcg cacgcccttg gacaccagca ggcgcgagat cagcgtgaag gtcggcggac
     2521 ccgcgtgggg tggccgccgc tggagccgcg cgaggtcgac gccggtgccg gggacgacgc
     2581 gccagcgcgc gcgacgcgac agcaggccca gcgactgcag cagcgcgggg tcatcggcgt
     2641 tctggaacac gatctcgtcg accagccgca gcgacatccc gtacgcgcgc cgggcgagcc
     2701 gggccctcgc cgtcgcgccg aacccgtggc tgtcgaacac ggcgccgagt ccgttgacca
     2761 gcggcacgac gcgctcgacc cgcgccagct tcgcggcgag cggggcgatc agcaccgcct
     2821 tgatctggta cacgagcacc acgtccgggc gctcggcgcg caggatcgag tgcaggtgac
     2881 gtgccgaacg cagatcctcg atcgggtcga tgcccgtccg gttcaccgac cacgcacggt
     2941 agctcgcgcc gagcgcggcg agcgggccgg ccatcacccc ggcaggttcc ggcgacacga
     3001 cggtgacgcg atggccgcgc gcgaggaccg cctccaggag ctcgcggcgc atgttggtcg
     3061 gcgtcgcgta cgtggtcacc atggcgagct tcatcgggct ttgattccac gtagttacgg
     3121 cctcgaggac ccggcggtct gggccattcg aacacgctgg ggccggcgag ccactaaccg
     3181 ccgagcgtcg tgcaagccca gcggactcat agcggcacct ggcgtcaggt gatgcgcagg
     3241 cccgcgctcg tcacgtggac aatctacctc gtcctgttcc cgctgtacat cttccgcagc
     3301 ggactgccgc agcccggcga cctgctcgtg atcgtcctgg tcccgctcgc gctgcagcgc
     3361 tggaacggcc ggctcggccg cgacatgcgc gccacgatgc gcgcgctgat ctggttcacg
     3421 ctgtgggtct gtctggtcga ctacacctgg gtgctcgtca tgggcagctt cacgctgttc
     3481 ggcccggaca gcttcctgct gttcccgctc tactatctct acaactcgct gatcttcctc
     3541 gtcgcgctcg tgctgtaccg ccggtatggc gagctgttcc tgcgggtcac ggtgttcacg
     3601 gtcaccgcga tggtgttcat ccaggtcctg tcgtcggtcg tgctcgggtc gagcagcgcg
     3661 cgcggctcgg tgttcttcaa caaccccaac cagctcggct actacgcgtt gctcgccgcg
     3721 acgctgatcg cgctcacgta tcgccggctc cagctccggc tgtggaccgc gagcgccgcg
     3781 ctggtgtgct gcggtttcct ggcgctgatc tcggcgtcgc gcgcgtcggc cggtggggtc
     3841 gccgtgctgc tcgtgctcat gctgttctcg aaccccaaga tcctcgtggc ggcgtgcctc
     3901 gccggcgccg ccatggtcag cggggtcggc ccgtttgccg acgcgatcga ggcatcgcag
     3961 gaccgcgtcc tgaacctcag cagccactcc gacgcctcgt tcttccagga gcgcggctac
     4021 gaccggatct ggcgcaacca gcagtacctg ctgctcggcg ccggtgaggg cggcctgtcg
     4081 cggttcgacg acacggcgta cgtcaagaac atggagatcc actcgtcggc cgggacgatc
     4141 ctgttcagct acgggatcgt cggatcgatc ctgttcctgg tgttcctgtg gcggctgctc
     4201 cgcggcgcgc gcgcccggct ggcgatcacg ctgctgccgc cgctgctcta caccggggcg
     4261 caccagggcc tgcggttcac gatgctgtgg gccgtgctcg cggtgttcgt cgcgctcaag
     4321 gacgacgtcg accgccctcg cctggaggcc gcgtgagggc gatgaccgtc gaccgagccg
     4381 tcccgcccat gcggcggatc acgtacggcg acccagcgcc ggcttgtccg ttgacttacc
     4441 gatcccctga tccgatcctc gaggagcagc catgtcgata aaatcgctta tgaggtccgc
     4501 cgtcgtcgtg agtgcgctgt cggggctgag cgcgtgcgcg gggctcgacc tgtcatcgac
     4561 cgagcagaac atcgatgctc cggccaacgt caccgcgacc ccgctcacgg acacccagat
     4621 ctcggtgtcg tggaccgcag tccccggcgc gctcgcatac ttcgtgctcg acaagaagtc
     4681 gaccgacacg accttcgcgt tcgtcggcac gacgctcgac ccgggcacct cgcagctggt
     4741 gatcgggctg acccccagca cgagctacga cttcgaggtc atcacggagt cgaccgccat
     4801 caccttctcc acgccctcgg cgccggtgtc ggccaccacg ttcgcggtgg cgcccggcgc
     4861 accgaccaac gtcgtcgcca ccgcggtctc caactccgag atcgatctga gctggaccaa
     4921 cttcccgggc gcgacggcgg tgtacgtgct cgagtcgcag gccggcggcc cgaacaactt
     4981 cctcaccacc gtgctggcgc ccggcgacac gttcgcggcg accggcctca tcgcgaacac
     5041 gatgtactgc tatcagctgg tgagccacct ggcggacggc accctgtctg gcctgtcgac
     5101 gcccccggcg tgcgcgacga ccggggccgg cccgcagccg cccaccgggt tgatcgcgac
     5161 cgcgatctcc gacacgcgca tcctcgttca gtggcaagcg gtggccgacg ctgcgaagta
     5221 cttcgtgcgg gaatcgcaga gcggcggccc gttcacgctc gtcggagtca acctcgccgg
     5281 cgatcagtcg ttcaccgccg tcaacctgca gccgtccacc agctactgct tcaccgtgtc
     5341 cacggtggtc gcggatggca cggagtcggc cccgtcctcg ctggcgtgcg ccacgacgct
     5401 cggggtcaac aacaacggcg gcttcgaggg ttactggaag ctcgacgagc gcaccggggc
     5461 cacggcgctc gattcgtccg gctggaaccg gcacggaacg atcacgaacg cggcctactc
     5521 gctcggcgac cgggcgcagc tcgatgacaa ccgctcggcg atctcgttca cggccagccc
     5581 ggactcggcg atcaccgtgg cgccggcgcc cgccttcgac ctgctcgacg tgttcaccgt
     5641 cgcgttctgg gccaagctcc cggccaccgc cgatgtgacg ttcatcggga tgcgcggcgc
     5701 cggctgcggc gcgctcggct gggagatcgc acaggacgcc agcggcctgc acttcgccgg
     5761 gcagacccag accgtcgccg ccggcgcgtc ggtcccggtc ggcgtgtgga cccacgtcgc
     5821 ggtcacgtac gcgagcggga cgatgcacac gttcgtgaac ggggtcgaga ccgccagcgg
     5881 cgcgttcacg ccggacaaca cgctgcaggc gccgctgaac atcggccacg tggcgggttg
     5941 cgcgggcggc gccgtgctca tggatgacgt gctgatcctc ggccgcgcga tgtcgggcac
     6001 ggaggtcagc gcgatcggaa cgctgccgcc gccaccgacc aatgtccacg tgacatcgaa
     6061 gacctcggcc tcgctggtct tcgcgtggga tccggtcccc ggcgcggtgc agtacatcat
     6121 ctcgaagggc acggcccccg ggaacgagac cttcttcacc cactcccccg cggattcgcc
     6181 gtcgttcgga gatggcaacc tcaccccgaa cacgcagtac tcgtacaacg ttcgcgcggt
     6241 cgtgggtaac ctgttctcga acccgtcgaa cgaggtcgtc gacaccacca agccgccgcc
     6301 ggcgccaccg accaacgtga ccgcgacggt gtccccaccc gaccggatcc aggtccagtg
     6361 gaccgcggcg cccgatgcct tcgtgtactt cgtcttcatg tcgaccgccg ggggtccgtc
     6421 cacgttcgcc ggcagcgtcg tggcgcccgc cacgacgttc gaggcggtca acctgagccc
     6481 ggcgacgacc tacacgttcc aggtcgagac cgaggacagc gtcctgaccg tcgggccgaa
     6541 gtcacagccc gcaagcgcga cgaccccgtg aagcactggg gggtcagctt cgcgtcggcc
     6601 ccttccatcg cctcgggtcc gttcaagtgg tcgttgacgt cgacggagcc ggatcggtgg
     6661 aggttccggc ttcatcgatc catcatcgac gcgacctccg cgctgcgggt ccgcggcggg
     6721 ctcaccctgt ctcgctggct gcaccggatg gtcggtgact acaccgagcc gctcaccgcc
     6781 gaggcgggtc gagacatccg gttgagcttt tccgggttcg atcggtactg gatgcgcgtg
     6841 ttctttcgcg gcgacttcta cgagcccgag ctctatcact ggtttcgtcg ggtctcgcga
     6901 ctgcgggact tcgcattcat cgacggtggc gccaacatcg gattctggag cgcgctgctc
     6961 accgctgccg agtttggcat cacgcgtgcg gtcgcgatcg aagcatcccc gacgacctac
     7021 gccgacctga gccgcaccgc ggccctgtgc ggacagcggt tcgcgacgga gcaccgcgcg
     7081 ctgaccgcca cgtccacacc cgtgatcttc gaacaaggtg ttggtcacgc aagcaggcat
     7141 atcgtggcgt cggatacacc gcgaacatcg gcgagccggc acgtggtggt cgaaggcacc
     7201 accgtcgacg ccctggtcgc caggtacggt ttcgacccac ggttcctcat cgtcaagctg
     7261 gacgtcgagg gcgccgagtt cgcgagcatc accgggggca gctctgcgtt ctcgaatggc
     7321 gcgatcttca tctacgagga tcacggcaag gatccgcgct cggtattgac cgagcagctg
     7381 ctcgacttcg gagcagcctg ctggttcatc gccgatgacg gttcgatgat cccggtcgac
     7441 agcgcgaccg cggcgtcgca gttcaagctc gaccaccgcc gtggctacaa ttatctgtgc
     7501 acgtcacgaa cgtatggagc caagcatcgc ctcgaagacc gactgttcgg gtgaagccgc
     7561 tacgaccgcg ccagctcggc gatcgcctgc tcgtagcgcc gcgcgatccg gccggtctcg
     7621 aaccgcgtgc gtaccgagtc gcggatcgca ccgccggtga gctgcgcccg cgtgcgcgtg
     7681 gcgagcgcga gcgcgtcggc gagcccggcg acgtcgtcga ccggcgcgag ccagccgttg
     7741 acaccgggca ggatcagccc ggcgacgccg gggc
//
LOCUS       DASVOR010001242         3029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_480497,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001242 DASVOR010000000
VERSION     DASVOR010001242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3029
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480497"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..1521)
                     /locus_tag="VF516_23665"
     CDS             complement(241..1521)
                     /locus_tag="VF516_23665"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEX8110758.1"
                     /translation="MTAKLVLPDSKEATLPVIVGTEDEHAIDIRNLRQETGFITLDNG
                     YMNTGSTTSAITYLDGEQGILRYRGYPIEELAEKSSFVETSYLLIKGELPTPSERENF
                     GRMLTRHSLIHEDMHQFFAGYPPTSHPMAVLSAMVTSLSAYYPDCLDRHSPMDLHITR
                     LLSKVRTIAAFSYKKSIGQPLIYPKNSLNYCGNFLHMMYAVPSEEYEVSPVLEKALNQ
                     LLILHADHEQNCSTSTVRMVGSSGANLYAAIAAGILALWGPLHGGANQAVIEMLEEIK
                     ASGGDFKKFLDDVKNRRDNRRLMGFGHRVYKNYDPRAKILKKMCDEVLAHLGIKDPLL
                     DLAKGMEEIALRDDYFVSKKLYPNVDFYSGIIYRAMGIPTNMFTVMFALGRLPGWIAH
                     WKEMVDDKETKIGRPRQIYTGQKIRHYVPIEQRQ"
     gene            complement(1738..2859)
                     /locus_tag="VF516_23670"
     CDS             complement(1738..2859)
                     /locus_tag="VF516_23670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110759.1"
                     /translation="MTRFTSFAIAAPLALALSGGLVGSARAQSQAELAAKANEEGKEL
                     MYADKYAEAAKKFQEAVARVPEAKYFVNLCTARLQEGKLDEALTACNAVDLNNPTPDQ
                     KDRAAKLIERINQEADKQHLALHPGGGGGGNTNLVQPSNPNNPNPPVYTPAVGKPLET
                     NLVTASPPANRYTWTLGFDFFGGGGRIGQPDFYGSAVFGGRLKGDVIVDAVHQVGIQG
                     YIQASQFTQGQNQTSLSESLEVADIGAALYKHICLGRTPRACFTPLAGIQLSLMGPVD
                     DMDSAGSQLFNYAAVGGRFELAFTYAFGPRLEHAISVMGGANVYSPVFSGPGPNDASG
                     ELTATERGLDTWGAAGYFGLGYTYRFNTPIGSSPFIILE"
BASE COUNT          461 a          956 c         1048 g          564 t
ORIGIN      
        1 ccaacgtcga tggtgggctc aacgtcggac gttgccgtcg acctcaacgc tccgggtcga
       61 cgatcaggtc aacgtcgacg atcacgaacg ccctatcgaa gacttgtgac gaatcatcag
      121 acgtcgccgt cgacctcaac gttcgggtca aggtccacgt catcgtcaaa gtcatcgcga
      181 gacggcagcg gcttgaccgg catccgccgc cgccagtatc agatcgagat cgagatcgcg
      241 ttactggcgc tgctcgatcg ggacgtagtg ccggatcttc tggccggtgt agatctggcg
      301 cggccggccg atcttggtct ccttgtcgtc gaccatctcc ttccagtggg cgatccagcc
      361 cggcaggcgg ccgagcgcga acatcaccgt gaacatgttc gtcgggatcc ccatcgcgcg
      421 gtagatgatg ccgctgtaga agtcgacgtt ggggtacagc ttcttcgaca cgaagtagtc
      481 gtcgcgcagc gcgatctcct ccatgccctt ggcgagatcg agcagcggat ccttgatgcc
      541 gaggtgggcg agcacctcgt cgcacatctt cttgaggatc ttcgcgcgcg gatcgtagtt
      601 cttgtacacg cggtggccga agcccatcag gcggcggttg tcgcgcctgt tcttgacgtc
      661 gtcgaggaac ttcttgaagt cgccgcccga ggccttgatc tcctcgagca tctcgatcac
      721 cgcctggttg gcgccgccgt gcagcgggcc ccacagcgcc aggatgccgg ccgcgatcgc
      781 ggcgtagagg ttcgcgcccg agctgccgac catgcgcacg gtcgaggtcg aacagttctg
      841 ctcgtggtcg gcgtgcagga tcagcagctg gttgagcgcc ttctccagca ccggcgagac
      901 ctcgtactcc tcgctcggca cggcgtacat catgtgcagg aagttgccgc agtagttgag
      961 cgagttcttg gggtagatca gcggctgccc gatgctcttc ttgtaggaga acgctgcgat
     1021 cgtgcggacc ttggacagaa ggcgcgtgat gtggaggtcc atcggcgagt ggcgatcgag
     1081 gcagtcgggg tagtacgccg acagcgaggt caccatcgcg gacagcaccg ccatcgggtg
     1141 gctggtcggc gggtagcccg cgaagaactg gtgcatgtcc tcgtggatca ggctgtgccg
     1201 ggtcagcatc cgcccgaagt tctcgcgctc ggaaggggtc ggcagctcgc ccttgatcag
     1261 caggtacgag gtctcgacga agctcgactt ctcggcgagc tcctcgatcg gatagccgcg
     1321 gtagcgcagg atgccttgct cgccgtcgag gtacgtgatc gcgctcgtcg tcgagcccgt
     1381 gttcatgtac ccgttgtcga gggtgatgaa gccggtctcc tggcggaggt tgcgaatgtc
     1441 gatcgcgtgc tcgtcctcgg tgccgacgat caccggcagc gtggcttcct tgctgtcggg
     1501 gagcaccagc ttagcggtca acttctgatc cgaaccggcc ataggatctc ctgctcgtgg
     1561 ctggaaaatg cagcgcggag accatacccg atctcgatcg agccatgacg tcgtgcggca
     1621 acgaaacgtg gcgccggtga cgcaaagtca cggcgctggt cgcgccgatc gaggatcgac
     1681 cgagcgccga tcagggaacg cccgggttcc ttcgggggag cgaggcacgc ggccgcgcta
     1741 ctccaggatg atgaacggcg agctgccgat cggtgtgttg aaccggtagg tgtagccgag
     1801 cccgaagtag ccggccgcgc cccaggtgtc gagcccgcgt tcggtcgcgg tgagctctcc
     1861 ggaggcgtcg ttgggcccgg gtccggagaa cacggggctg tacacgttcg cgccgcccat
     1921 caccgagatc gcgtgctcca ggcgcgggcc gaacgcgtag gtgaacgcga gctcgaatcg
     1981 cccgccgacg gcggcgtagt tgaacagctg gctgccggcg ctgtccatgt catcgaccgg
     2041 tcccatcagc gacagctgga tgccggcgag cggcgtgaag cacgcccgcg gcgtgcggcc
     2101 gaggcagatg tgcttgtaga gcgcggcgcc gatgtcggcg acctccaggc tctcggacag
     2161 gctggtctgg ttctggccct gggtgaactg cgaggcctgg atgtagccct ggatgccgac
     2221 ctggtggacc gcgtcgacga tgacgtcgcc cttgagccgg ccgccgaaca ccgccgagcc
     2281 gtagaagtcg ggctggccga tccgtccgcc gcccccgaag aagtcgaagc cgagcgtcca
     2341 ggtgtagcgg ttcgccggtg ggctggccgt gacgaggttg gtctccagcg gcttgccgac
     2401 ggccggcgtg tagaccggcg ggttggggtt gttcgggttg ctgggctgga cgaggttggt
     2461 gttgccgcca ccgccgccgc cggggtggag cgccaggtgc tgcttgtcgg cctcctggtt
     2521 gatgcgctcg atcagcttgg cggcgcggtc cttctggtcc ggggtcgggt tgttgagatc
     2581 gaccgcgttg cacgcggtca acgcctcgtc gagcttgccc tcctgcagcc gggcggtgca
     2641 caggttgacg aagtacttgg cctcgggcac gcgggcgacg gcctcctgaa acttcttcgc
     2701 cgcctccgcg tacttgtcgg cgtacatcag ctccttgccc tcttcattgg ccttggccgc
     2761 gagctctgcc tggctctgcg cgcgcgcgct cccgaccaac ccccctgaca gcgcgagcgc
     2821 cagcggcgcc gcgatcgcga acgaagtgaa cctggtcatg acgatcggac ccttcccgga
     2881 agacggaccc cgcggatgtt accatcctac gcggcgcgtc gacgcggtct tcccggtgcg
     2941 agctttttca cgctcgtgcc cggggcgcgc cgagccgtgc cgggggcctt gccggccgtt
     3001 gtgatcgagg gcgcggtcga tcagccggc
//
LOCUS       DASVOR010001243         7989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_275039,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001243 DASVOR010000000
VERSION     DASVOR010001243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7989
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275039"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..672)
                     /locus_tag="VF516_23675"
     CDS             complement(202..672)
                     /locus_tag="VF516_23675"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8110760.1"
                     /translation="MVKFLSTVDSRFWLRQRLAPSGIDDSIGMIWEGTDNQIGSGGIE
                     LSLFAGGPVAQNAIDSPDPWGFYAPTLDNDWRGYLQATSSGSTHFQNWEAQPFIMGGY
                     SCPQPGEATSVWPNLFNGWADRLVFAGEHACPGFFGFMEGALESGIVAAFRLTQ"
     gene            complement(666..1124)
                     /locus_tag="VF516_23680"
     CDS             complement(666..1124)
                     /locus_tag="VF516_23680"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8110761.1"
                     /translation="MSRSLYARLHRRFGARPTGLALARAVRARVDAFRTKFGFPLVRK
                     PAATGPRVAVIGGGFAGTSAGWMLAQLGATVTLYEASDRLGGRVWSQPDFTSERTIEW
                     GGELIGYNHPAWILLAEFAQLGLSMLTTEDAYDSLGLSMPMVPTRRWAPW"
     gene            1628..2611
                     /locus_tag="VF516_23685"
     CDS             1628..2611
                     /locus_tag="VF516_23685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX8110762.1"
                     /translation="MILIGLAICTLAATQARAATSGFTITSSSSVQFWVNDAPWADLH
                     YQINGGVQYDVTAVPGGATVRYFFTIGSSTGGATDTAWSQFTMTAGGSGNYVVVWEDT
                     FDGSGQPSTANWNYHVGNGFNPGAQAFDGWGNGEWEWYRPEQSTRQNGNLVIRADYFS
                     TPTVIAGRNWFQRSGRLTTKGKRSWTYGRIEARIAMPNATATWPAFSMMGDAYDDTST
                     TNFNAPITQFDTMASNWSSCGEIDIMEHRNSETVTFQNPDCFRGPAGRPPSSRARSAS
                     VRSPSSISTRSSGSQRRSAGTSTARPIPIRSTRWTSPRPIRRSSTSHSTSS"
     gene            complement(2879..3610)
                     /locus_tag="VF516_23690"
     CDS             complement(2879..3610)
                     /locus_tag="VF516_23690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110763.1"
                     /translation="MSTEALVWPASEGTVNTQLGYLVSAGIYLPGVRANGTDISIETG
                     EATVYWGFPDEDSLHWLATLGPGDMFHVDGIPSDSVLSVQADSPFTYRIALPPASDPG
                     PGGAPADAIPSTQAFWRCNLPGCTFPDWTGAVIGWPSWAAYQTNARSGDQSRSVFSAD
                     GVPLYPYMGSWAQSCEITAESGVVQIIEWQRGTDVWRETWLYPRQSHVIDLVSPEDGA
                     MIETYDGSPGFSVSLRNCTPQPISP"
     gene            complement(4234..5187)
                     /locus_tag="VF516_23695"
     CDS             complement(4234..5187)
                     /locus_tag="VF516_23695"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8110764.1"
                     /translation="MGIPRQALLLRLAIATTALAAIGLALAYVVAWIPVWPWALLEHF
                     RVQYVALGVIATAAAAVLRVRGYLDVAAIATLLHALPVTADLCRTPQLELADGVALHV
                     LVLNVHTESSSFDQVRQLIADEHPDIVGLLEVDQRWIVGVAPALAGYTGRIEAPRGDN
                     FGIALYARGHLAGSAEELGSSLPSVVAELSIREAQLRVILVHPPPPMSAGNLAEQYDE
                     VAAIARRVAAREPTIIMGDFNATPWSKPFVELVARSGLCDSRAGFGIQPSFPTASALL
                     RIPIDHLLVSCAVGVRDRRIGRDVGSDHLPVILDLAIPRSR"
     gene            5763..6794
                     /locus_tag="VF516_23700"
     CDS             5763..6794
                     /locus_tag="VF516_23700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110765.1"
                     /translation="MREPRAEGRGPGQAPSSTAGGLGPGKRTLTEALPAAGEADAAQS
                     YSPPGPLPPTRAELEATEHARLQAVEPTKTATPDPAKGPGDKADDKAPPDMTGIGATV
                     AVDRFIVAAKDVQKNWGTLKTEERADRLGKAANAELKAAGCYEVTPNLEDLSEAGKFH
                     FSTWVLGLGKAPFGAASVDDSQAAEIADTVYHESRHAEQWHRMARLLAGKGTSAADIM
                     KKLGIAGKAAADAATKPLKDATTAEGKEASAWYESVYGSGAAHRNKLLGETLPKYSKL
                     LEDAAAEYGKVTADKAATKDQKDAAYKKWVAAYDAYKKEAGDPYHALPEEADAWAVAG
                     KMKAAYTKK"
     gene            6749..7195
                     /locus_tag="VF516_23705"
     CDS             6749..7195
                     /locus_tag="VF516_23705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110766.1"
                     /translation="MGGRRQDEGRVHQEMTARTFLIALVAAGCASGARVDNQPASTVR
                     DPMDTISTLKEVCEVLTRDPLSPAAAREALGRSGAAWVRAGRVIAAVDAPEPNHVELD
                     LPAPVALEAGRLAGHDDDRPRPPRYRIGHIDLVIAIVARGTGSVTA"
     gene            complement(7185..>7989)
                     /locus_tag="VF516_23710"
     CDS             complement(7185..>7989)
                     /locus_tag="VF516_23710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110767.1"
                     /translation="DDAPIASQTVTFTCLGDPCPWGDSVSTEALVWPASEGTVNTQLG
                     YLVSAGIYLPGVRANGTDISIETGEATVYWGFPDEDSLHWLATLGSGDTFHVDGIPSD
                     SVLSVQADSPFTHRIALPPSSDPGPGGAPADAIPSTQAFWRCNLPGCTYPDWTGAVID
                     WPSWAAYQTNARSGDQSRSVFSADGTPLYPYMGSWAQSCEVTAESGVVQIIEWQRGTD
                     VWRETWLYPRQSHVIDLVSPEDGAMIETYDGSPGFSVSLKNCTPQPITP"
BASE COUNT         1379 a         2740 c         2737 g         1133 t
ORIGIN      
        1 gcccgacccg tccgcccagg cgagcgcggc gccggcaagc tcgccgggcc cgtccgccag
       61 cctgggcggc ttcccgcaga tcctggtgct ccgcttcggc tgatccgcgg cgaaccgggg
      121 atctggacgc ccgccccctg agacgatcgc gcagcgaagc gcgacgtgcg cagaccttcc
      181 gacgcggtat acggcgccgg actactgcgt gaggcggaac gcggcgacga tgccggattc
      241 gagcgcgcct tccatgaagc cgaagaagcc gggacaggcg tgctccccgg cgaacaccag
      301 gcgatccgcc cacccgttga agaggttcgg ccacacgctg gtggcctcgc cgggttgcgg
      361 gcaggagtag ccgcccatga tgaacggctg cgcttcccag ttctggaaat gggtgctgcc
      421 ggacgacgtg gcttggaggt agccgcgcca gtcgttgtcg agcgtgggcg cgtagaatcc
      481 ccacggatcg ggggaatcga tcgcgttctg ggcgaccggg ccgccggcga acaggctgag
      541 ctcgataccg ccgctgccga tctggttgtc ggtgccctcc cagatcatgc cgatggagtc
      601 atcgatgccg gagggcgcga gccgctgcct gagccagaag cggctgtcga cggtgctgag
      661 gaacttcacc acggtgccca tcgtcgggta ggcaccatcg gcatcgagag accgagcgag
      721 tcgtaggcgt cctcggtcgt gagcatgctg aggccgagct gcgcgaactc ggcgagcaag
      781 atccaggccg ggtggttgta gccgatgagc tcgccgcccc attcgatcgt ccgctccgat
      841 gtgaagtccg gctggctcca gacccggccg ccgaggcgat cggacgcttc gtagagcgtg
      901 acggtggcgc cgagctgggc gagcatccag ccggccgacg tgccggcgaa cccgccgccg
      961 atcacggcga cgcgcgggcc ggtggccgcc ggcttgcgga ccaggggaaa gccgaacttg
     1021 gtgcgaaacg cgtcgacgcg cgcgcggacc gcgcgcgcga gcgcaaggcc agtgggacga
     1081 gcgccgaacc tgcggtggag acgggcatac agagagcgcg acatgtctcc cacggtaccg
     1141 aaatacgctc gcgctggcca ttcgattgat gggcacaaca ggtaaacgct attagccgat
     1201 actggagtta tccagtgtac ccaaatatcc tctgggagtg gagcgatacg ctgcacggca
     1261 gacatcgatt cgaatcgacg gatgcgcgct tcgcgttacc gggcatctgc tgcgttatcg
     1321 gccgtaatcc gtcgtcattg acatgaccgc gcagcgtggc tccgcaatga ccggacaacc
     1381 agggagcgcg cgcggtgacg agcgcccccg cggctggaca agccagcgca cagcgccgcg
     1441 aacgtcgtat catcgcgatc gccgcggcgg acgcctccga gcccaaccat gtcgagctgg
     1501 acctgcctgc gccggtcgcg attgcatgtg gacgggcaat cgcgagggga taatactgcg
     1561 cgcctaccgg aggcaggcca tgcacactca tcgtctcgcg cggattcgtc cctaggcgtt
     1621 gtcgactgtg atcctcatcg gactggcgat ctgcacgctc gcggccacgc aggcgcgtgc
     1681 cgcgacatcg ggcttcacga tcacgtcgag cagcagtgtc cagttctggg tgaatgatgc
     1741 gccgtgggcc gatctgcact accagatcaa cggcggagta cagtacgacg tgaccgcggt
     1801 gcccggcggc gcgaccgtcc gctacttctt cacgatcggc agctccaccg ggggcgccac
     1861 cgacacggcg tggtcgcagt tcacgatgac cgccggcgga agcggcaact acgtcgtggt
     1921 ctgggaggac acgttcgacg gcagcggcca gccaagcacc gcgaactgga actaccacgt
     1981 cggcaatggc ttcaatccgg gcgcccaggc gttcgacggc tggggcaacg gagaatggga
     2041 gtggtatcgc cccgagcagt cgacccggca gaacgggaac ctggtgatcc gcgcggacta
     2101 cttctcgacg cccacggtga tcgccgggcg caactggttc cagcgctcgg ggcggctgac
     2161 gaccaaaggc aagcggtcgt ggacctacgg gcggatcgag gcccggatcg ccatgcccaa
     2221 tgcgaccgcg acgtggccgg ccttctcgat gatgggcgac gcgtacgacg atacgagcac
     2281 caccaacttc aacgcgccga tcacgcagtt cgacacgatg gcgtccaact ggtcgagctg
     2341 cggcgagatc gacatcatgg agcaccgcaa cagcgagacg gtgacgttcc agaacccgga
     2401 ttgtttccgt gggccagcgg gacgaccacc cagcagccga gcacgttcgg cgtcggtgag
     2461 gtcacccagt tccatctcta cacgatcgag tgggagccaa cgcagatccg ctggtacatc
     2521 gaccgcacga ccgatcccaa tccggtccac acggtggaca tcaccgcgac caatcaggag
     2581 gagttccaca agccattcca catcatcctg aacctcgcgc tcggggggca ggtccccggt
     2641 acgcagccca accaggcgga tttcccgctg ttcatgatgg tggactacgt cagagtctgg
     2701 cagaagcagt gaaccgaacc ggcagaacga gcacgcctgg tagatcccct cggtgcccgc
     2761 actgttgcga tcggagccga agtagagcgt cagctcgtcg cctgggatgc gcgggcccag
     2821 cgctaccagc cgacggctga ccacgcggcg gcctgaccgc gcggtggtac gggcagcgct
     2881 acggtgagat cggctgcggc gtgcagttcc tcagcgatac actgaatccg ggcgagccat
     2941 cgtaggtctc gatcatcgcc ccgtcctccg gcgaaacgag atcgatgacg tgggactgtc
     3001 tgggatagag ccaggtctct cgccacacat cggtcccgcg ctgccattcg atgatctgca
     3061 cgaccccgga ctcggcggtg atctcgcagc tctgggccca ggatcccatg tacgggtaga
     3121 ggggcacacc gtccgcggag aacaccgagc gcgactgatc gccggatcgg gcattggtct
     3181 ggtacgccgc ccaggacggc cagccgatga ccgcgccggt ccagtcgggg aaggtgcatc
     3241 ccgggaggtt gcagcgccag aacgcctgcg tcgacggaat cgcatcggcg ggggcgccgc
     3301 cgggaccggg atcgctcgca ggcggcaggg cgatgcggta ggtgaacggc gagtcggctt
     3361 gcacgctcag caccgaatcg ctgggaattc cgtcgacgtg aaacatgtct cctggcccga
     3421 gggtcgccag ccagtggagg gaatcctcgt cagggaaccc ccagtacacg gtcgcctcgc
     3481 cggtctcgat cgagatgtcg gttccgttcg ccctcacgcc ggggaggtag atcccggcgg
     3541 agacgagata ccccagctgc gtattgacgg ttccctccga ggccggccac acgagcgcct
     3601 ccgtgctcac cgagtcaccc cacgggcacg ggtccccgag gcacgtaaac gtgacggtct
     3661 gcgaagcgat cggcgcgtca tccggaggcg tcccggcatc cggcgtcccg gcatccggcg
     3721 tcccggcatc cggcgtcctg gcatccggcg tcccggcatc cggcgtcctg gcatccgacg
     3781 tcccggcatc cggcgtcccg gcatccggcg tcccggcatc cggcgtcccg gcatccggcg
     3841 tcccggcact gggcgttcct gcgggatccg gcggcgtcac gccgaccggc gatccgcaac
     3901 cagtctgcgc cacgacgcac gagatccaga ggaaagcgga ccatgtcgcg cagcgacggc
     3961 cgacccggga acccctcggt ttcacgcgtg tgcctccggt gagccgcgca ttcgttcaaa
     4021 cgttgagcct acgtgtgctg gttccggatt caccaggtct cgcccgctcc tcacgtgctg
     4081 cgcgatgacg ggtggctcag ggacacgccg catggcgcgt tcagagcttc cgcacgagga
     4141 ggcagctccg ccggatcggg tgctcgacgc ccggatacgg ctcgcgatcc aggcgcgcct
     4201 cgatcgcgaa ttcggcggcg ggctcacgcg gcgtcaccgc gagcgcggaa ttgccagatc
     4261 gaggataacg ggcaaatggt ccgatccgac gtcgcgcccg atccgccggt cgcgcactcc
     4321 gaccgcgcac gacaccagca ggtgatcgat cgggatgcgc agcaacgccg acgcggtggg
     4381 gaagctgggc tggatgccga aacccgcgcg gctatcgcac agcccactac gcgccacgag
     4441 ctcgacgaac ggcttcgacc acggcgtcgc gttgaagtcg cccatgatga tcgttggctc
     4501 gcgggcggcc acgcgccgcg cgatcgcagc gacctcgtcg tactgctccg ccaggttccc
     4561 ggcgctcatc ggcggcggcg gatggacgag gatcacgcgg agctgcgcct cgcggatcga
     4621 cagctcggcg accacgctcg gcagcgagct cccgagctcc tcggccgacc cggcgaggtg
     4681 gccccgcgcg tacagcgcga tgccgaagtt gtccccgcgc ggcgcctcga tccgcccggt
     4741 gtacccggcc agcgccggcg cgaccccgac gatccagcgc tggtcgacct cgagcagacc
     4801 gacgatgtcc ggatgctcgt cggcaatcag ctgccgcacc tgatcgaagc tcgagctctc
     4861 ggtgtgcacg ttgagcacca gcacgtggag cgccaccccg tcggcaagct ccaactgtgg
     4921 cgtccggcac aggtcggccg tcaccggcag cgcatgcagg agcgtcgcga tcgccgccac
     4981 gtcgaggtag ccccgcaccc ggaggaccgc ggccgccgcg gtcgcgatca ccccgagggc
     5041 gacgtactgg acccggaagt gctcgagcag cgcccacggc cacaccggga tccatgcgac
     5101 gacgtacgcc agcgcgaggc cgatcgccgc cagcgcggtc gtcgcgatcg ccagccgcag
     5161 cagcagggct tgccgtggaa tccccatctc tcgtgctgta gcacgcgggg tgcgcgacgc
     5221 gcccgtcatc cgtctccggg cgccgggacc aagccaagca tgaacgcgag gtcgtcgcca
     5281 ggggcttcga tgaaccagct cgacgagcag gcggttggcg cgagcgaacg gaggtgggcc
     5341 cgggcgtgcg gccccgcggc ggtggcggtg cgctcggtca gcacgcgctt gcgagcagcg
     5401 atctcggctc gtctgctgcg ccttcaatcc gatttcattg atgtcaccga cacagatagc
     5461 catctccgta gtctgacaat gtcgtattgc tcacgagctc ccctgctcta ggatgcctcc
     5521 ggtgacgtcg cgccatcgtc cggtctcgct cgggcccacc gaggtcgacg tgatccgtcg
     5581 gcccgacggc gccgtcctcc tccggtcgcc gcaccggctg gggccgtttc caccgcggat
     5641 caccgaccag ctcgaccact tcgcggcgct cgccccggat cccgtgctgt tcgcccagcg
     5701 agcacgcgcc tccgcggatt gcgacaggcg agcttccacg agcgccatga tacgatgccg
     5761 ggatgaggga accgcgtgcg gaaggccgtg gaccgggaca ggcccccagc agtaccgcgg
     5821 gcgggctagg gcccggcaag cgcacgctca ccgaggcgct gccggccgcc ggggaagcgg
     5881 acgccgccca gagctactcg ccgccggggc cattgccacc gacccgggcc gagctcgagg
     5941 ccacggagca tgcccggctg caggccgtcg agccgaccaa gaccgccact cccgatccgg
     6001 ccaagggccc gggcgacaag gccgacgaca aggcgccgcc ggacatgacc ggcatcggcg
     6061 cgacggtggc ggtggatcgc ttcatcgtcg cggcgaagga cgtgcaaaag aactggggca
     6121 cgctcaagac cgaggagcgc gcggatcgcc tgggcaaggc cgcgaacgcc gagctcaagg
     6181 cggccgggtg ctacgaggtc acgccgaacc tcgaggatct gtcggaggcc ggcaagttcc
     6241 acttctcgac ctgggtgctc gggctcggca aggcgccgtt cggggccgcc tccgtcgacg
     6301 acagccaggc cgccgagatc gccgacaccg tgtaccacga gtcgcgccac gccgaacaat
     6361 ggcaccgcat ggcgcggctg ctcgcaggca agggaaccag cgcggccgac atcatgaaga
     6421 agctcggcat cgcgggcaag gccgccgcgg atgccgcgac caagccgctc aaggacgcga
     6481 ccaccgccga gggcaaggaa gcgtcggcgt ggtacgagag cgtgtacggc tcgggcgccg
     6541 cccaccgcaa caagctgctc ggcgagacgc tgcccaagta cagcaagctg ctcgaggacg
     6601 ccgccgccga gtacggaaag gtgacggccg acaaggcggc gaccaaggac cagaaggacg
     6661 ccgcgtacaa gaagtgggtc gcggcgtacg atgcgtacaa gaaggaagcc ggcgatccgt
     6721 accacgcgct tccggaagag gccgacgcgt gggcggtcgc cggcaagatg aaggccgcgt
     6781 acaccaagaa atgaccgcgc gcaccttcct gatcgcgctc gtcgcggccg gctgcgcgtc
     6841 cggcgcgcgg gtcgacaacc aaccagccag caccgtgcga gaccccatgg atacgatcag
     6901 caccctgaag gaagtctgcg aggtcctgac ccgcgatccg ctgagcccgg ccgcggcgcg
     6961 cgaggccctc ggccggtccg gcgccgcctg ggttcgcgcc gggcgcgtga tcgccgcggt
     7021 ggacgccccc gagcccaacc atgtcgagct ggacctgccg gcgccggtcg cgctcgaggc
     7081 tggtcggctc gctggtcacg acgacgatcg gccgcgtcct cctcgatatc gaatcggaca
     7141 catcgacctt gtgatcgcca tcgtcgcgcg cggtacgggc agcgttacgg cgtgatcggc
     7201 tgcggcgtgc agtttttcag cgatacgctg aatccgggcg agccatcgta ggtctcgatc
     7261 atcgcgccgt cctccggcga aacgagatcg atgacgtggg actgtctcgg atagagccag
     7321 gtctctcgcc acacatcggt cccgcgctgc cattcgatga tctgcacgac cccggactcg
     7381 gcggtgacct cgcagctctg ggcccaggat cccatgtacg ggtagagggg cgtgccgtcc
     7441 gcggagaata ccgagcgcga ctgatcgccg gatcgggcgt tggtctggta cgccgcccac
     7501 gacggccagt cgatgaccgc gccggtccag tcggggtagg tgcatcccgg gaggttgcag
     7561 cgccagaacg cctgcgtcga cggaatcgca tcggcggggg caccgccggg accgggatcg
     7621 ctcgaaggcg gcagggcgat gcggtgggta aacggtgagt cggcttgcac gctcagcact
     7681 gaatcgctgg gaattccgtc gacgtgaaac gtgtctcctg acccgagggt cgccagccag
     7741 tggagggaat cctcgtcagg gaacccccag tacacggtcg cctcgccggt ctcgatcgag
     7801 atgtcggtac cgttcgccct cacgcccggg aggtagatcc cggcggagac gaggtagccc
     7861 agctgcgtat tgacggttcc ctccgaggcc ggccacacga gcgcctccgt gctcaccgag
     7921 tcaccccacg ggcacgggtc cccgaggcac gtaaacgtga cggtctgcga agcgatcggc
     7981 gcgtcatcc
//
LOCUS       DASVOR010001244         9595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_129438,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001244 DASVOR010000000
VERSION     DASVOR010001244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129438"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="VF516_23715"
     CDS             complement(<1..1262)
                     /locus_tag="VF516_23715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110768.1"
                     /translation="MSSPATPLPAFRRVFHPGVRDDRGQLLGGTQVLHLVPHRGQLFC
                     SLSYKLQAEQPGDLPVGSQIAVLDHAAGRWQLDREYDRAQWRATLESVTFTQDGHGQA
                     LAQPVPLLLAAPSDNRGPVYVDSRDDRTGTWTRTHLGTGASAGGIRSFFVYRDKVTGQ
                     ERVFAGTVPLGLFSGTYDPDAPGQIRWDARPELDGYTRRPMAFAECNGRLYTTIKPDM
                     YRRIDGPSPRWEKVYTIPGPVIIPSCGLRGLTAVPRPGGGGQVLLATLEGDRCRMVRI
                     DPEDGFRETVELEVLDLLGERWGSRPRYGVVAYDDFTPVVDPSTGHTLLVSGLGATYS
                     TKVDWHPPDGWTDDAWYLIRHPDGRRYDVRRIETPASEPRPILVVVRTIAVSPFEPGT
                     LYFGGYDPNAKPARDTAWVYSAPIGAALG"
     gene            complement(1271..2089)
                     /locus_tag="VF516_23720"
     CDS             complement(1271..2089)
                     /locus_tag="VF516_23720"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110769.1"
                     /translation="MTGDAPRFVELPVDPDRVQAALEQPVYRDDRREIRVCDVIDRLQ
                     EAGMRAYIIGGAPRDWLLGLPGKDIDLSLDRPVEEAHRVLRAAYPDIDPLRGHFERFG
                     TLRWGSAELGGLDLNILRSWKDIQNDDMWTTTFVARTDLMEDIQTRDFSVNVFVYDCR
                     DRVLRDPLGGGLADLEARALRLVTHHRVLDTSYRTTFRICQFLARGYAPTPSVLAHLE
                     RYADHDIQGMGPRIHRWVPGHYAAGDERLARFQQLVYASARQPASIAVLDSFFQ"
     gene            complement(2086..2724)
                     /locus_tag="VF516_23725"
     CDS             complement(2086..2724)
                     /locus_tag="VF516_23725"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HEX8110770.1"
                     /translation="MIRRLEQRVRPEDLGGESVAQLVRRWSRLRSPEMDLKMRDHDPR
                     WPEVFQAERRRLQAALADEAVEIQHIGSTAIPMLPGKNIIDVAVAGRALPPSGRQIEL
                     LVELGYEPYGPSPIDPEFSWLWRIEAGGQGAVVVHLCAIHSPWLANLVNFRDFLCAFP
                     EERQRYADRKRELAAAADQSWIEYSVAKRVLALRITERANAWAAARSRALRT"
     gene            complement(2760..3620)
                     /locus_tag="VF516_23730"
     CDS             complement(2760..3620)
                     /locus_tag="VF516_23730"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEX8110771.1"
                     /translation="MVKPNFFMVGAPRCATTTMYTYLKQHPDIYLSLLKEPLYFGSDL
                     TRQPLAVTDEASYLSLFAGAGDARVIGEGSVFYLMSTRAAEELKQFSPSARILIMLRN
                     PVDMMHSLHALYLRTRNDELDDFDAALAAEADRAQGRRLPARCYFPEGLQYTRLAHYA
                     EPVGRFLRAFGRDRVHVVVFDDLTRDPAGEYRRLLAFLGLPDHPVELDVDKATELIRP
                     VVLRQMRAASPEIRRKLKTGRDAHRGPRSRPVSPELRARLAAELLPDVAQLSQLLDRD
                     LTHWCRSAAA"
     gene            complement(3624..4328)
                     /locus_tag="VF516_23735"
     CDS             complement(3624..4328)
                     /locus_tag="VF516_23735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8110772.1"
                     /translation="MELTRVALVTGGNRGIGLEVCRQLAARGVRTLLTARREDRGRDA
                     ARALAEQGLPVSFCPLDVTDEHSLERAVDYAVRELGRIDILINNAGVALDFEVLGLDV
                     AMSVVRTTMDINVYGPLRLTQLVVPVMRAQSYGRVVNVSSGLGSFSRITAKKLAYRLS
                     KASLNAMTRVFADELADTNILVNAVTPGWVRTHLGGIRAERSVEQGAEGILWLATLPD
                     DGPRGKFFKDRDEFPW"
     gene            complement(4342..5094)
                     /locus_tag="VF516_23740"
     CDS             complement(4342..5094)
                     /locus_tag="VF516_23740"
                     /inference="COORDINATES: protein motif:HMM:NF015527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HEX8110773.1"
                     /translation="MILTDDFVYIHQPKTGGTFVTRVLERLYQDGSRGPLVNTHKHGS
                     CSDVPDAWRGKPLITTIRNPYDRYVSQYRFAWWRRYPDQYCGEAAMREMFPHYPELSF
                     GEFLQLANTLFVGRHQGKDTGFHNANFSEDRRLGWHTEQFVRFYCVHPREVFAAITEG
                     YIAEGRCRQALFPIRILSAENLNAELHALLRELGHAPDAIAFVRDADRIYPAEGGRAP
                     GDHWETYYTPELKRLVRTRERLLFQLFPEYDV"
     gene            complement(5105..6388)
                     /locus_tag="VF516_23745"
     CDS             complement(5105..6388)
                     /locus_tag="VF516_23745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen-III oxidase family protein"
                     /protein_id="HEX8110774.1"
                     /translation="MTTTDHSEAPRIEMTRKGFVTNYPPYRYWRPEVAERFLEPKPLN
                     VYLHTPYCIQRCAYCHYKTTTLDDNRKAEIDRYVQALCKEIELASRRFHLAERKTISV
                     YFGGGTPTLLSKDNLHRIFDTLRANLAFADPEITVEGEPVTLIPAKAEFLQEMGVNRI
                     SLGVQSFSDQVVAKTGRRDTEKHALRAVEIAKATGAIVNVDLMSGLAGETDETWAHTV
                     ARALTTDVHSLTVYKTELYANTEYYVSIKKHALELPSDAEELRYAGYAIDELERHGYL
                     PVNFFTFTRGGGHVQRHTTSKWKGDDIYAFGVSAFGSLGNFSIQNTSELAQYTAMVEA
                     GELPIARGYHLAAKDLMARDVVLGMKLIHLDRKAFQHRHGFDLVRLCAPAVDELVADG
                     LITVTDERVSLTRKGILWGDFSGRKLAAAVDAIAC"
     gene            complement(6414..6746)
                     /locus_tag="VF516_23750"
     CDS             complement(6414..6746)
                     /locus_tag="VF516_23750"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8110775.1"
                     /translation="MNPKPAATDIDHASRHTAALTEIIATLRHMLVEELDVRLTAEMV
                     TDDVPLLERGLGLDSIVLYEFITLIEKRFGFEFADQSLDTKVFANLTVLAQHIHDATR
                     LPPQVAAS"
     gene            complement(6769..8274)
                     /locus_tag="VF516_23755"
     CDS             complement(6769..8274)
                     /locus_tag="VF516_23755"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX8110776.1"
                     /translation="MLSETFLQGDVPFRAVLSRALSAAQAHHSELSDGQLSLGYEALP
                     DHFAALDERFAAQGISPGEVVALECTTALPGALILLYLLSRGFSVVLLPPRDAAIQTP
                     VPRFCRWRLTVLSGLVSGPDSIALHRPETVLEIAEARDHHPAFAAQAYATGHLFLRTS
                     GSIGTPKLAVYTHRRLLGNAMHCIGRLRVTAQDRIAIPVPLAHMFGLGAALLPGIAAG
                     ASVRMIEGANLLRYLEHERVYRPTISFLTPALCAMFARQRRAPEHYRHVVVAGDRLTP
                     EVFEAAELRFRRVLNLYGSTEMGVISVADADRCDGPRSTTVGTPLPGVELRLDGVEEA
                     IEAPDGAGAIACRHPYGFEGYVDGDGGAWTGEPALRDGWYRTRDLGRLHPGGFLEVLG
                     REDHSVNRDGRLVVLAEVERAMEQLPGVERAVTVLGSDHLRGRAIVAYCALRDGHGLD
                     ASRVRDACAGVLPSYAIPDHIVITSAMPLLANGKVDRKALAAEAGNHGRAR"
     gene            8664..>9595
                     /locus_tag="VF516_23760"
     CDS             8664..>9595
                     /locus_tag="VF516_23760"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8110777.1"
                     /translation="MPSLREVSSNLLTGALDIEKAYPMMRLVAEPIAPGGTFVVSSAN
                     VTAFETDDGLVLIDTGGFLSADRTRAALRSAVAAPAHTAIWTHGHVDHVFGVELYERE
                     AGRTVRVIAHEAVSRRFARYRLTHGWNEAINTRQFQFAVKFPSEFRAPDETFTDQRNV
                     EVGGRRFELHHALGETDDHAWVWVPDAGVLCTGDLFIWGAPNCGNPQKVQRYPREWAA
                     ALRAMDALGAEVLCPGHGVPIWGAADVHRALDDTASYLESLVDQVVTLMNRGARLDEV
                     IASVAPPAALAARPYLSPRYDEPEFIVRNLYRLYG"
BASE COUNT         1473 a         3265 c         3385 g         1472 t
ORIGIN      
        1 ccgagcgcgg ccccgatcgg cgccgagtac acccaggccg tgtcgcgcgc gggcttggcg
       61 ttgggatcgt agccgccgaa gtacagcgtc cccggctcga acggagacac cgcgatggtg
      121 cgaacgacga cgaggatcgg ccggggctcg gaggcaggag tctcgatgcg ccgcacgtcg
      181 tagcggcgac catcggggtg ccggatcagg taccaggcgt cgtcggtcca cccatcgggg
      241 gggtgccagt cgactttcgt gctgtaggtc gcgcccaggc ccgaaaccag cagcgtgtgg
      301 ccggtgctcg gatcgacgac cggcgtgaag tcgtcgtatg ccaccacgcc atacctgggg
      361 cggctgcccc agcgctcgcc gagcagatcg agcacctcga gctccacggt ctcccggaag
      421 ccgtcctccg gatcgatacg caccatgcga cagcgatcgc cctccagcgt cgcgagcagc
      481 acctggcctc cgccgcccgg ccggggcacg gccgtgagcc cgcgcagccc gcagctggga
      541 atgatgaccg gcccggggat cgtgtagacc ttctcccagc gcggcgacgg tccgtcgatg
      601 cgccggtaca tgtccggctt gatcgtcgtg tacagccggc cgttgcactc ggcgaacgcc
      661 atcggccgcc gggtgtagcc gtccagctcc ggccgggcgt cccagcggat ctgaccgggc
      721 gcgtccggat cgtaggtccc gctgaacagc ccgagcggca ccgtgccggc gaacacgcgc
      781 tcctggccgg tcaccttgtc gcgatacacg aagaagctgc ggatgcctcc ggcgctggcc
      841 ccggtgccca ggtgcgtccg cgtccacgtc ccggtgcggt cgtcgcggct gtcgacgtag
      901 accggcccgc ggttgtccga gggcgcggcc aggagcagcg gcaccggctg cgcgagcgcc
      961 tggccgtggc cgtcctgggt gaacgtcacc gactcgagcg tcgcccgcca ctgcgcgcgg
     1021 tcgtactcgc ggtccagctg ccagcggccc gccgcgtggt ccagcaccgc gatctgcgag
     1081 ccgaccggca gatcgcccgg ctgctccgcc tgcagcttgt aggacagcga gcagaacagc
     1141 tggccgcggt gcggtacgag atggagcacc tgggtaccgc ccaggagctg accgcggtcg
     1201 tcgcgtacac cggggtggaa gacgcggcga aacgcgggca gcggagtcgc gggcgatgac
     1261 atgggagacc tcactgaaag aacgagtcga ggaccgcgat cgacgcgggc tggcgggcgg
     1321 acgcgtagac cagctgctgg aacctcgcga gccgctcgtc cccggcggcg tagtggccgg
     1381 ggacccaccg gtggatgcgc ggtcccatcc cctggatgtc gtgatcggcg tagcgctcga
     1441 ggtgtgcgag cacgctgggc gtcggcgcgt agccccgggc caggaactgg cagatccgga
     1501 acgtcgtccg gtagctggtg tccagcaccc ggtggtgcgt gaccagccgg agcgcccggg
     1561 cctcgagatc cgcgagcccg ccgccgagcg gatcccgcag cacgcgatcg cggcagtcgt
     1621 agacgaacac gttgaccgag aagtcgcggg tctggatatc ctccatcagg tcggtgcgcg
     1681 cgacgaacgt ggtggtccac atgtcgtcgt tctggatgtc cttccaggac cgcaggatgt
     1741 tgagatccag gccgccgagc tcggcgcttc cccagcgcag cgtgccgaac cgctcgaagt
     1801 gcccgcgcag cgggtcgatg tcggggtagg ccgcgcgcag cacgcggtgc gcctcctcca
     1861 ccggccggtc cagcgagaga tcgatgtcct tgcccggcag gccgagcaac cagtctcgcg
     1921 gggcgccgcc gatgatgtac gcgcgcatgc cggcttcctg gagccgatcg atcacgtcgc
     1981 acacgcggat ctcgcgccgg tcatcgcggt agaccggttg ctcgagggcg gcctgcacgc
     2041 gatcggggtc gaccgggagc tcgacgaagc gcggcgcgtc gccggtcatg tgcgcagcgc
     2101 ccgggatcgg gcggcggccc acgcgttggc ccgctcggtg atgcgcaggg cgagcacgcg
     2161 cttggccacg ctgtactcga tccagctctg gtcggccgcg gcggccagct cgcgcttgcg
     2221 atcggcgtag cgctgccgct cctcggggaa cgcgcacagg aagtcgcgaa agttcaccag
     2281 gttcgccagc cacgggctgt ggatcgcaca caggtgcacc accacggcac cctggccgcc
     2341 ggcctcgatc cgccacagcc aggagaactc cgggtcgatc gggctcgggc cgtaaggctc
     2401 gtagccgagc tccaccagca gctcgatctg gcgcccggac ggaggcagcg cacgaccggc
     2461 caccgcgaca tcgatgatgt tcttgccggg cagcatcggg atcgccgtgc tcccgatgtg
     2521 ctggatctcg acggcctcgt cggccagcgc cgcctggagc cggcgccgtt cggcctggaa
     2581 gacctccggc cagcgcggat cgtggtcgcg catcttcagg tccatctcgg gagaccgaag
     2641 ccgcgaccac cggcgcacga gctgcgccac cgactcgccg cccaggtcct cgggacgcac
     2701 gcgctgctcg aggcggcgga tcatgcgggc cagcccgaga gcgtccaccg cgcgcccggt
     2761 cacgcggccg cgctccggca ccagtgcgtg agatcgcggt ccaggagctg gctcagctgc
     2821 gccacgtccg gcagcagctc ggcggcgagc cgggcgcgca gctcgggcga cacggggcgg
     2881 ctgcgcggac cgcggtgagc gtcacggccg gtcttgagct tccggcggat ctccggcgac
     2941 gccgcgcgca tctggcgcag caccacgggc cggatcagct cggtcgcctt gtcgacgtcg
     3001 agctccaccg ggtggtcggg caggccgagg aacgccagca agcgccggta ctcgccggcg
     3061 gggtcgcgcg tcagatcgtc gaacacgacc acgtgcacgc ggtcgcgacc gaacgcgcgc
     3121 aggaagcgcc ccacgggctc ggcgtagtgc gcgagcctcg tgtactgcag gccctcgggg
     3181 aagtagcagc gcgcaggcaa ccgccgaccc tgcgcgcgat cggcctcggc cgcgagcgcg
     3241 gcgtcgaagt cgtcgagctc gtcgttccgg gtccgcaggt agagcgcgtg cagcgagtgc
     3301 atcatgtcca ccgggttgcg cagcatgatc aggatccgcg cggacggcga gaactgcttg
     3361 agctcctcgg ccgcccgggt cgacatgagg tagaacaccg agccctcgcc gatcacccgg
     3421 gcatcaccgg cgccggcgaa caggctcagg tagctggcct cgtcggtgac cgcgagcggc
     3481 tgccgggtca gatccgatcc gaagtagagc ggctccttga gcagtgacag gtagatgtcc
     3541 ggatgctgct tgaggtacgt gtacatcgtg gtcgtcgcac accgcggcgc tccgaccatg
     3601 aagaagttgg gcttgaccat gggctaccag ggaaactcgt cgcggtcctt gaagaacttg
     3661 ccgcgcggcc cgtcgtcggg gagcgtggcc agccagagga tgccctcggc gccttgctcg
     3721 accgagcgct cggcgcggat cccgccgagg tgggtgcgta cccagcccgg ggtcaccgcg
     3781 ttgaccagga tgttggtgtc ggcgagctcg tcggcgaata ccctggtcat cgcgttgagc
     3841 gacgccttcg acagccggta ggcgagcttc ttcgcggtga tgcgagagaa cgaccccagc
     3901 ccgctcgata cgttgaccac gcggccgtag ctctgcgcgc gcatcaccgg gaccaccagc
     3961 tgggtgagcc gcagcgggcc gtacacgttg atgtccatcg tcgtgcggac gacgctcatc
     4021 gcgacgtcga ggccgaggac ctcgaagtcg agcgcgacgc ccgcgttgtt gatcaggatg
     4081 tcgatgcgcc cgagctcgcg gacggcgtag tcgaccgccc gctccaggct gtgctcgtcg
     4141 gtcacgtcga gcggacagaa gctcacgggc aggccctgct cggccagcgc gcgggcggcg
     4201 tcccggcccc tgtcctcccg gcgagcggtg agcagcgtgc ggacgccgcg cgccgcgagc
     4261 tggcggcaga cctcgagccc gatgccgcgg ttgccgccgg tgaccagtgc gacgcgggtc
     4321 agctccatgt tgcgatctcc gtcatacgtc atattcgggg aacagctgga acagcagccg
     4381 ctcccgggtc cggaccaggc gcttgagctc cggtgtgtag taggtctccc agtgatctcc
     4441 cggtgcgcgc ccgccctcgg ccggatagat ccgatcggcg tcgcgaacga acgcgatcgc
     4501 gtcgggtgcg tggccgagct cgcgcagcag cgcgtggagc tcggcgttga ggttctccgc
     4561 cgacaggatc cggatcggga acagggcttg ccggcagcgg ccctcggcga tgtagccctc
     4621 ggtgatcgcg gcaaacacct cgcgcggatg cacgcagtag aaccgcacga actgctcggt
     4681 gtgccagccc agccggcgat cctcggagaa gttggcgttg tgaaacccgg tatccttgcc
     4741 ctggtggcgg ccgacgaaca gcgtgttcgc gagctgcagg aactcaccga agctgagctc
     4801 ggggtagtgc gggaacatct cccgcatcgc ggcctcgccg cagtactggt ccggatagcg
     4861 cctccaccac gcgaaccggt actgcgagac atagcgatcg tacgggttgc ggatcgtcgt
     4921 gatcagcggc ttgccgcgcc aggcgtccgg aacgtcgctg cagctcccgt gcttgtgggt
     4981 attgaccagc gggccgcgac tgccgtcctg gtagagccgc tccagcacgc gggtcacgaa
     5041 cgttccaccg gtcttgggct ggtggatgta cacgaagtcg tcggtgagga tcatggcgcg
     5101 gggctcagca ggcgatggcg tcgaccgccg cggcgagctt gcgcccggag aaatcacccc
     5161 acaggatgcc cttgcgggtc agcgacacgc gctcgtcggt gacggtgatg aggccgtccg
     5221 cgaccagctc gtccaccgcc ggtgcgcaca gccgcaccag atcgaagccg tggcggtgct
     5281 ggaacgcctt gcgatcgaga tggatgagct tcatgcccag gacgacgtcc cgggccatca
     5341 ggtccttcgc ggccagatga tagccccgcg cgatcgggag ctcgcccgcc tcgaccatcg
     5401 cggtgtactg cgccagctcg ctggtgttct ggatcgagaa gttgcccagc gatccgaacg
     5461 ccgagacgcc gaatgcgtag atgtcgtcgc ccttccactt gctggtggtg tggcgctgca
     5521 cgtggccgcc gcccctggtg aacgtgaaga agttgaccgg caggtagcca tggcgctcga
     5581 gctcgtcgat cgcgtagccg gcgtacctga gctcctcggc gtcggacggc agctcgagcg
     5641 cgtgcttctt gatgctgacg tagtactcgg tgtttgcgta cagctcggtc ttgtagaccg
     5701 tcagcgagtg cacgtcggtg gtgagcgcgc gcgccacggt atgtgcccag gtctcgtcgg
     5761 tctcgcccgc cagcccgctc atcaggtcga cgttgacgat cgcgccggtg gccttggcga
     5821 tctcgaccgc gcgcagcgcg tgcttctcgg tgtcccgccg cccggtcttc gcgacgacct
     5881 ggtcggagaa cgactggacg ccgaggctga tccggttgac ccccatctcc tggagaaact
     5941 cggccttggc cgggatcagc gtgaccggct cgccctcgac cgtgatctcc gggtccgcga
     6001 atgccaggtt cgcgcgcagc gtgtcgaaga tgcggtgcag gttgtccttc gacagcagcg
     6061 tgggcgtgcc tccgccgaag tacaccgaga tcgtcttgcg ctccgcgagg tggaagcgac
     6121 gcgacgcgag ctcgatctcc ttgcacagcg cctggacgta tcggtcgatc tccgccttgc
     6181 ggttgtcgtc gagcgtcgtg gtcttgtaat ggcagtacgc gcaacgctgg atgcagtacg
     6241 gcgtgtgcag gtagacgttg agcggcttgg gctcgagaaa ccgctcggcg acctccggcc
     6301 gccagtagcg atacggcgga tagttggtca cgaacccctt ccgggtcatc tcgatgcgcg
     6361 gtgcctcgct gtggtcggtg gtggtcatgg cgcggctggc tcggtggagt ggatcacgat
     6421 gccgctacct gcggcggcag gcgcgtcgcg tcgtggatgt gctgcgccag gacggtgagg
     6481 ttcgcgaaca ccttcgtgtc caggctttga tcggcgaact cgaagccgaa gcgcttctcg
     6541 atcagcgtga tgaactcgta cagcacgatc gagtcgagcc cgaggccgcg ctcgagcagc
     6601 gggacgtcgt cggtcaccat ctcggcggtg agacgcacgt cgagctcctc gacgagcatg
     6661 tgccggagcg ttgcgatgat ctcggtgagt gccgccgtgt gacgcgatgc gtggtcgatg
     6721 tcggtcgctg caggcttcgg gttcatctgg atggtccttg ttcgggcgtc accgcgctcg
     6781 cccatgattg ccggcctcgg cagccagcgc cttgcggtcc accttcccgt tggccagcaa
     6841 tggcatggcg gacgtgatca cgatgtggtc cggaatggcg tacgacggca gcacgccggc
     6901 acacgcatcg cgcacgcgcg acgcgtccag cccgtggccg tcgcgcagcg cgcagtacgc
     6961 cacgatcgcg cggccgcgca ggtgatcgct cccgagcacg gtgaccgcac gctccacgcc
     7021 tggcaactgt tccatggcgc gctcgacctc ggcgagcacc acgaggcggc cgtcccggtt
     7081 cacgctgtgg tcctcgcgcc cgagcacctc gaggaacccg ccggggtgca gccggcccag
     7141 gtcgcgggtg cgataccagc cgtcgcgcag cgcgggttcg cccgtccacg ccccgccgtc
     7201 gccgtccacg taaccctcga agccgtacgg gtgacggcag gcgatcgccc cggcgccgtc
     7261 cggagcctcg atggcctcct ccaccccatc gagtcgtagc tcgacgccgg gcaacggcgt
     7321 accgacggtg gtcgatcgcg ggccatcgca gcggtccgcg tccgcgaccg agatgacacc
     7381 catctcggtg ctgccgtaga ggttgagcac gcggcggaac cgcagctcgg cggcctcgaa
     7441 gacctccggc gtgagccggt cgccggccac cacgacgtgg cggtagtgct ccggcgcgcg
     7501 gcgctggcgc gcgaacatcg cgcacagcgc cggtgtgagg aagctgatcg tcgggcggta
     7561 tacgcgctcg tgctcgaggt agcgcagcag gttggcgccc tcgatcatcc gcacgctggc
     7621 gcccgccgcg atgcccggca gcaacgccgc gccgagcccg aacatgtgcg ccagcggcac
     7681 ggggatcgcg atccgatcct gcgccgtcac ccggagccgc ccgatgcagt gcatggcatt
     7741 gccgagcagc cggcggtgcg tgtacaccgc gagcttgggt gtcccgatac tgcccgatgt
     7801 ccgcaggaac aggtgaccgg tggcgtaggc ctgtgccgcg aacgccgggt ggtggtctcg
     7861 ggcctcggcg atctcgagca cggtctcggg gcggtgaagc gcgatgctgt cggggccgga
     7921 caccaggccg ctgagaaccg tcagccgcca gcggcagaac ctcggcaccg gggtctggat
     7981 cgccgcatcc cgaggcggga gcaacacgac actgaatcct cgggacagca gatacaggag
     8041 gatcagggcg ccggggagcg cagtggtgca ctcgagcgcc accacctcgc cgggcgagat
     8101 accctgcgcg gcaaaccgct cgtcgagcgc ggcgaagtga tcgggcagcg cttcgtagcc
     8161 cagcgacagc tggccatcgc tcagctcgct gtgatgagcc tgtgctgccg agagcgcccg
     8221 cgacagcacg gcgcggaacg gaacgtcacc ctgcaagaag gtctcggaaa gcattgctca
     8281 tcgcctcgag gagcgtagcc acacacacga ggtctgtgcg ccactgcaac gtcctcgaaa
     8341 catcttggag cgtaactcta gatgtggcga actgtcacgt gcaatgcaaa aagttgctgc
     8401 gtcagcagac tcgatcgcag ccttacatca atgcgccacc atcaatgcgc catcatcaat
     8461 gcgccaccac cggtgtgcct caatacgaac cggggctctg gtgatcacga ccacccgctg
     8521 cggccgctgc tgccacggtt gacgatgcgt gcacggccaa tgacgccacg cacatcatgg
     8581 ccgatgaact cacactgaac ttgagtcaca gcagcgcatc ggtgcggagc agttcgacgg
     8641 tcgccggtga tacgattcgc gccatgccga gtctccggga ggtgtcctcg aacctgttga
     8701 ccggcgcgct cgacatcgag aaggcgtatc cgatgatgag gctggtcgcc gagccgatcg
     8761 cgcccggcgg cacgttcgtt gtcagctctg ccaacgtgac cgcattcgag accgacgacg
     8821 ggctcgtgct gatcgacacc ggtgggttcc tgtccgccga tcggacgcgc gcggcgctcc
     8881 gcagcgcggt cgccgcgccc gcccacaccg cgatctggac ccacggccac gtcgatcacg
     8941 tgttcggcgt cgagctctac gagcgcgagg ccgggaggac agtccgtgtg atcgcgcacg
     9001 aggccgtgtc gcgccggttc gcgcggtacc gtctgaccca cggctggaac gaggcgatca
     9061 acacgcgaca gttccagttt gcggtgaagt tcccgtcgga gttccgcgcg cccgacgaga
     9121 cgttcaccga ccagcgcaat gtcgaggtcg gcgggcggcg cttcgagctc caccacgcgc
     9181 tcggcgagac cgacgatcac gcctgggtct gggtgccgga cgccggggtc ctgtgcacag
     9241 gggatctgtt catctggggg gcgccgaact gtggcaatcc gcagaaggtg cagcgctatc
     9301 cgcgcgagtg ggcggccgcg ctccgggcga tggatgcgct cggcgccgag gtcctgtgcc
     9361 ccggtcacgg cgtgccgatc tggggcgcgg cggacgtgca ccgcgcgctc gacgacacgg
     9421 cgagctacct cgagtcgctg gtcgatcagg tcgtgacgct gatgaaccgc ggcgcgcggc
     9481 tcgacgaggt gatcgcgagc gtcgcgcccc cggccgcgct cgccgcccgg ccgtacctgt
     9541 cgccaaggta cgacgagccc gagttcatcg tccgcaacct gtaccggctc tacgg
//
LOCUS       DASVOR010001245         3651 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363348,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001245 DASVOR010000000
VERSION     DASVOR010001245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363348"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF516_23765"
     CDS             <1..203
                     /locus_tag="VF516_23765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110778.1"
                     /translation="HRLEGARPDGAEHIHRWRAEVLAAAGRIDDARAALDRASAEIAA
                     KATKLRDPELRRLYLTSRQLPR"
     gene            complement(220..888)
                     /locus_tag="VF516_23770"
     CDS             complement(220..888)
                     /locus_tag="VF516_23770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110779.1"
                     /translation="MSKAETGEVEEQRARMEQQEERLTRALELLTQRGPGPAGRRRRD
                     WDALAAVIASFIGLMALAVSAYTAYVQREQLRAQAWPHLQLYSSNVDSTMGHSVANQG
                     TGPASVIAMRVRVDGVPATTWGDVWTAVGYARGKGFITSTISHAVIPAGKDIAFARPA
                     DDDQSRAKFAELLLREKHSFGVTLCYCSVLDECWIASNEIAMNGWAITRDGCPITVDQ
                     RFVE"
     gene            complement(1210..1578)
                     /locus_tag="VF516_23775"
     CDS             complement(1210..1578)
                     /locus_tag="VF516_23775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110780.1"
                     /translation="MATNQIRAVTFRPVAAGAGADSEGGHALFMTLDVAEPARLVGFL
                     EDVITRFKVEQISGPPDARFLLITVVGDVSAAEFADAWRAATAHDAPARALLGALHQA
                     DVMQGDAHGGVLGQASLLAG"
     gene            1669..2319
                     /locus_tag="VF516_23780"
     CDS             1669..2319
                     /locus_tag="VF516_23780"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX8110781.1"
                     /translation="MALARSIAPIPGRRVLVALLIIFAAAVVPAVIVPTLACRPADPQ
                     LQDLGPVGAFGLTDERGEPFTDAALRGHATLVSFLFTRCDTICPVTTMKMARLQDRTF
                     DAGARIKLVSFSVDPAYDTPARLAEYAQRYHADPTRWRFVTGPVEDMRRLVEGAFMNS
                     MASEGVTPSGAPAISHSGYFLLIDGNARIRGAYDSSDIRKLDEMERAARYLVRTGR"
     gene            2339..>3651
                     /locus_tag="VF516_23785"
     CDS             2339..>3651
                     /locus_tag="VF516_23785"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8110782.1"
                     /translation="MTTWTRRGFLGATGGLVIGFALPVRRRPYLAEAAAAGEPATVTA
                     FLRIGTDDVVTVMANHSEMGQGVWTTIPMLIAEELEADWSKIRVEHAPAAPEFARPGG
                     RGQGTGGSSSTRSEFERLRQVGATARMMLIEAAARRWKVPAERLRAEAGHVIGPRDRL
                     SFGQLAADAAKLTPPAKVALKDPASWKIIGKPMRRLDSPEKITGRAVFGFDVQLPGLL
                     TAVVRRPPTFGGQVKSFRADAALRVPGVRQVVGVPSGVAVIADHFWAAKVGRDALEVD
                     WDPGPGGEFSTDGYLARLRELVHQRGEPAADKGNADAALAGAARRIEAQYELPYLAHA
                     PMEPLNATVRIAPDACEIWTGTQSQTGDQRAAAEILGLAPEKVAIHTTFLGGGFGRRA
                     ALGQDFVREAVEVARAAGATVKTVWTRDDDLRGGRYRPMFVHRVEG"
BASE COUNT          492 a         1331 c         1326 g          502 t
ORIGIN      
        1 tccaccgcct cgagggcgcc cggcccgacg gcgccgagca catccaccgc tggcgcgccg
       61 aggtcctcgc cgccgccggc cggatcgacg acgcgcgcgc cgcgctcgac cgcgccagcg
      121 cggagatcgc tgccaaggcc accaagctgc gcgaccccga gctccgccgg ctctacctca
      181 ccagccgcca gctgccgcgg tagcggcggc gcctctccat cactcgacga accgctggtc
      241 gaccgtgatc gggcagccgt cgcgggtgat cgcccacccg ttcatcgcga tctcgttcga
      301 agcgatccag cactcgtcga gcaccgagca gtagcacagc gtgaccccga agctgtgctt
      361 ttcgcgaagc agcagctccg cgaacttcgc gcggctctgg tcgtcgtcgg ccggtctcgc
      421 gaaggcgatg tccttgccgg cgggaatcac cgcgtggctg atcgtcgagg tgatgaagcc
      481 tttgccgcgt gcgtagccta ccgccgtcca gacatctccc caggtcgtgg ccggcacgcc
      541 gtccacgcgc acgcgcatcg cgatcacgga ggcgggtccg gtaccctggt tcgcgacgga
      601 gtggcccatg gtggaatcga cgttgctgga gtagagctgc aggtggggcc aggcctgggc
      661 gcgcagctgc tcgcgctgca cgtacgcggt gtaggccgag accgccagtg ccatcaaacc
      721 gatgaacgac gcgatcaccg ccgccagcgc gtcccagtcc cgtcgccgcc tgccggccgg
      781 cccggggccg cgctgggtga gcaactcgag cgcgcgggtc aaccgctcct cctgctgctc
      841 catccgcgca cgttgctctt cgacctctcc agtctcagcc ttggacacga tcgcctcctg
      901 gatgctccgg gcggcgaagg atatcctgac actgccccgg gcacaggcgc ttcgaatgcc
      961 gaccgcgccg gacagatatg gagtcgtcca gtcttcgccg cgacatgatg tggtatcgca
     1021 agcatcgccg gtcgctgctg cgacggtacc gcgggcaata cgtggcgatc atcgatgccg
     1081 aggtgatcga ccacggcccc gagttccccc cactggcggt tcgcgtgttt gcccggtatg
     1141 gcaaccgggc catctacatg ccccgtgtcg agggtcgcga tcgcgtgatc cgcatccgct
     1201 cgccgcggct cagccggcga gcagcgaggc ctggccgagc acgccgccgt gggcgtcgcc
     1261 ctgcatgacg tcggcctgat gcagggcgcc gagcagcgcg cgggcgggcg cgtcgtgcgc
     1321 ggtggcggcg cgccaggcgt cggcgaactc ggcggccgag acgtcgccga cgacggtgat
     1381 cagcaggaac cgcgcgtcgg gcgggccgga tatctgctcg accttgaacc gggtgatgac
     1441 gtcctcgagg aagccgacca ggcgggcggg ctcggcgacg tcgagcgtca tgaacagcgc
     1501 atggccgccc tcactgtcgg cgcccgcgcc cgcggccacg gggcggaacg tcacggcgcg
     1561 gatctggttg gtcgccacgg ggttacgcta gcacgcgcgc cgggctgcga aatgttgtcg
     1621 caccggcggg cccgtgcatc cggcccgcgg atctgccacg gtccgggcgt ggcgctcgcc
     1681 cgctccatcg caccgatccc gggacgccgg gtgctggtcg cgctgctgat catcttcgcc
     1741 gccgcggtgg tgccggcggt gatcgtgccg acgctggcgt gccggcccgc ggatccccag
     1801 ctccaggacc tcggcccggt cggcgcgttc gggctgaccg acgagcgcgg cgagccgttc
     1861 accgacgcgg cgctgcgcgg ccacgcgacg ctcgtgagct tcctgttcac gcgctgcgac
     1921 acgatctgtc cggtgacgac gatgaagatg gcgcggctgc aggacaggac gttcgacgcc
     1981 ggcgcgcgga tcaagctggt gtcgttctcg gtcgacccgg cctacgacac gccggcgcgg
     2041 ctcgccgagt acgcgcagcg ctaccacgcc gatccgacgc gctggcggtt cgtgaccggg
     2101 ccggtcgagg acatgcgccg gctggtcgag ggggcgttca tgaactcgat ggccagcgag
     2161 ggcgtgacgc cgtcgggcgc gccggcgatc tcgcacagcg gctacttcct gctgatcgac
     2221 ggcaacgccc gcatccgcgg cgcctacgac tcgagcgaca tccgcaagct cgacgagatg
     2281 gagcgcgccg cgcgctacct ggtgcgcacc ggccgctgat cggactagac tcgccgccat
     2341 gacgacctgg acgcggcgcg ggttcctcgg cgctaccggc ggcctcgtga tcggcttcgc
     2401 gctgccggtc cgccgccggc cctacctcgc ggaggcggcg gcggccggcg agcccgcgac
     2461 cgtcacggcg ttcctgcgga tcgggaccga cgatgtcgtg acggtgatgg ccaaccactc
     2521 cgagatgggc cagggggtgt ggaccacgat cccgatgctg atcgccgagg agctcgaggc
     2581 cgactggtcg aagatccggg tcgagcatgc cccggcggct ccggagttcg cgcggcccgg
     2641 tgggcgcggc cagggcaccg ggggctcgag ctcgacgcgc tccgagttcg agcggctgcg
     2701 ccaggtcggc gcgaccgcgc ggatgatgct gatcgaggcc gcggcacggc gctggaaggt
     2761 cccggccgag cggctgcgcg ccgaggccgg ccacgtgatc ggcccccgcg accggctgtc
     2821 gttcggccag ctcgccgccg acgccgcgaa gctgaccccg cccgccaagg tcgcgctcaa
     2881 ggatccggcg agctggaaga tcatcggcaa gccgatgcgg cggctcgaca gccccgagaa
     2941 gatcaccggc cgcgcggtgt tcggcttcga cgtccagctc cccggcctgc tcaccgcggt
     3001 ggtccggcgc ccgccgacgt tcggcggcca ggtcaagtcg ttccgcgccg acgcggcgct
     3061 gcgggtgccc ggcgtccgcc aggtcgtcgg cgtgccgtcg ggcgtcgcgg tgatcgccga
     3121 tcacttctgg gcggcgaagg tcggccgcga tgcgctggag gtcgactggg atccgggccc
     3181 cggcggcgag ttctcgaccg acggctacct cgccaggctg cgcgagctgg tccaccagag
     3241 gggcgagccg gcggccgaca agggcaacgc cgacgccgcg ctcgccggcg ccgcccggcg
     3301 gatcgaggcg cagtacgagc tgccctacct cgcgcacgcg ccgatggagc cgctcaacgc
     3361 cacggtccgc atcgcgcccg acgcgtgcga gatctggacc ggcacgcagt cgcagaccgg
     3421 cgaccagagg gccgccgccg agatcctcgg cctcgcgccc gagaaggtcg cgatccacac
     3481 gacgttcctc ggcggcgggt tcggccggcg cgccgcgctc ggccaggact tcgtgcgcga
     3541 ggcggtcgag gtcgcgcgcg ccgccggggc gacggtcaag acggtgtgga cccgcgacga
     3601 cgacctgcgc ggcggccgct accggccgat gttcgtgcac cgtgtcgagg g
//
LOCUS       DASVOR010001246         1973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_392222,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001246 DASVOR010000000
VERSION     DASVOR010001246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392222"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF516_23790"
     CDS             <1..207
                     /locus_tag="VF516_23790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110783.1"
                     /translation="RSRCDSAAPRSVDAALSTPPHPGVPRNAAGRNLVQSRTARAANC
                     SVMRVISDDAHRGIARPSCAAATR"
     gene            436..1296
                     /locus_tag="VF516_23795"
     CDS             436..1296
                     /locus_tag="VF516_23795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110784.1"
                     /translation="MALSRLLLLSVSLPLFASACATDDDVTDDAIIEPGSSFHMLPLT
                     TGDKTQNAITPRIAGLTFFGGPVLKNVNVHPVFWNSTTKLQSNINSFYPAVVGGSPLY
                     KMLAQYSSIGSGTAVAGIVDTSTTASVRDSTVQSRISALITAGRLPAPTANTYYPVHF
                     PSGMTITAPDGSRSCVQFCAYHGTFVRNGVNVNYGIIPDIGQSGCNGGCGGSTVTNNT
                     DSVASHELVEATTDPAVGLATVFGPPLAWYDQVDNGEIGDLCNGIQGTTAGFVVQKEW
                     SNAAHACVDH"
     gene            complement(1359..1871)
                     /locus_tag="VF516_23800"
     CDS             complement(1359..1871)
                     /locus_tag="VF516_23800"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8110785.1"
                     /translation="MTERPGPQRPLSLGAGVVVVHDGRVLLVRNIRGVTRGRYLLPAG
                     RIEPGELPDHAAAREAFEETNLRVAVEGLLGVRLWVMDDGEHNYFFMFLARLVSPASD
                     LRPNLAEIDDARFFSPEEMAALGRDETWSGAIAIAHKALDPGRTLWPNDAGLSDRSGV
                     DVPDRWRIWM"
BASE COUNT          328 a          711 c          621 g          313 t
ORIGIN      
        1 cgcagccggt gcgattccgc cgcgccgcgt agcgtcgacg cagcattgtc gacaccgccg
       61 cacccgggtg tgccgcgtaa tgctgcgggg cgcaatcttg tgcaatcgcg caccgcgcgc
      121 gcagcaaatt gctccgtcat gcgcgtgatc tccgacgacg cgcaccgcgg catcgcgcgc
      181 ccgtcgtgtg ccgcggcgac gcggtgacag gcactgacac tccagggtag cactgacctg
      241 cgtcgacgct taaccatgat taagcgcagc cggcgttgcg gccgacatgt cggtattaca
      301 gatcgaatgt gactgactgc tcggttggga cgggagcaac tgatccccat agggcaaaat
      361 cccgatctgg cagttgacgg cagtgttcgc aataccgtat gtgtcgctcc ccccccatct
      421 ctggaggact ctgacatggc gctttcccgt ttgctgctcc tgtccgtatc gctgccactg
      481 ttcgcctcgg cctgcgcgac cgatgacgac gtcaccgacg acgccatcat cgagcccggc
      541 agcagcttcc acatgctgcc gctgaccacc ggcgacaaga cccagaacgc gatcacgccg
      601 cggatcgccg gcctgacctt cttcggcggt ccggtcctca agaacgtcaa tgttcaccct
      661 gttttctgga actcaacgac gaaactgcaa tccaatatca attcgttcta cccggcagtc
      721 gtcggcggca gcccgctgta caagatgctc gcgcagtaca gcagcatcgg gtccggcacc
      781 gccgtcgccg ggatcgtcga caccagcacc accgccagcg tgagggacag cacggtccag
      841 tcgcggatca gcgcgctgat caccgccggt cgactgcctg ccccgaccgc gaatacgtat
      901 tatcccgtac acttcccgtc ggggatgacg atcaccgcgc cggacggcag ccggtcgtgc
      961 gtccagttct gcgcctacca cggcaccttc gtgcgcaacg gcgtcaacgt caactacggc
     1021 atcatcccgg acatcgggca gtcgggctgc aacggtggct gcggcggcag cacggtcacc
     1081 aacaacaccg actcggtcgc gtcgcacgag ctggtcgagg cgaccacgga tccggcggtc
     1141 ggcctggcca ccgtgttcgg cccgccgctc gcctggtacg accaggtcga caacggcgag
     1201 atcggcgacc tgtgcaacgg gatccagggc accaccgcgg gcttcgtcgt tcagaaggag
     1261 tggtcgaacg cggcgcacgc ctgcgtcgat cactgatcct ccgctgattc tctgctgagc
     1321 tgatgcgccg ccgatccggc ccggatccgg cggcgtttct acatccagat ccgccagcga
     1381 tcggggacgt cgacgccgga gcgatccgac agcccggcgt cgttgggcca caaggtcctc
     1441 cccgggtcga gcgccttgtg cgcgattgcg atcgcgcccg accaggtctc gtcgcggccc
     1501 agcgcggcca tctcctccgg ggagaagaac cgcgcgtcgt cgatctcggc caggttgggc
     1561 cgcagatcgc tcgccggcga caccagccgg gcgaggaaca tgaagaagta gttgtgctcg
     1621 ccgtcgtcca tcacccacag gcggacgccg agcagcccct cgaccgcgac gcgcaggttg
     1681 gtctcctcga acgcctcgcg cgccgccgca tgatcgggca gctcgccggg ctcgatccgc
     1741 cccgccggca gcaggtagcg gccgcgggtc acgccgcgga tgttgcgcac gagcaggacg
     1801 cggccgtcgt gcaccaccac caccccggcg ccaaggctca gcggccgctg cgggcctggg
     1861 cgctctgtca tgcgcgcgac cttgccccgg ctgggcgcga gacacaaggg ccgtccggcc
     1921 aggtccggcc agcgaggttc cccgaaatcg tcccggagtc cgcccggata ggc
//
LOCUS       DASVOR010001247         5116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421221,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001247 DASVOR010000000
VERSION     DASVOR010001247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421221"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3195)
                     /locus_tag="VF516_23805"
     CDS             complement(<1..3195)
                     /locus_tag="VF516_23805"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8110786.1"
                     /translation="MAFNKDKVMDAARKFVDKGQIDKAVKEYLRIVHEDPKDVRVWLK
                     IGDLYAKKGAKQDAIETYLKVARFYHDQGFLHKSVAVYKQIVKLDPRLVDVILKLAEL
                     YRELRMMPEAMQHFESVAAHFHREGNTKEALAAVKKLVDIDPENIATRIKLAELYSKE
                     GLVDDAATEFQTACEQLRRQGRQDDFLKVAERLLWHKPNNQALNRELAGLYLRRNDPR
                     RALQKLQACFKADPRDVETLGLLAQAFQALDQKAKTASVLKELARIHVENKHKELAGE
                     VFRKILELLPGDPDAIAFLGPSAQAGSRPSQSPIPRAVTSLPAQIRAQAGDARFNLTG
                     DMPPYEAASARMTGSMPLIDEQSLSGIDFALPEYDDADFSADMEPPPDPRAMSAAGER
                     HAEEIQKILAETDVYVKYGLHQKAIDHLRRVFTLDADNVEARERLKDIFLSQNREAEA
                     EAELMRLAELTAPSDPDRAELYLQELLSLSGTHTGAYDLARRFRLRVARMSSASPDIE
                     VSSDYGAIALADGEFDAGRSSRDSIDEFDPDELIGAMAHGSPAPGRPPRRPNGGYPAE
                     ADNFELDFEPSPSQATRQMSPEIVEALASSHADELDGPLGGRLGGPVIVDRPRWAESL
                     DDARALDAQLEDIELGDEIDRHVARELARGAGKPVDITEDVADDLPFDPDEARAFDAG
                     VPRASSQVAAVSRAMEPGGTDAIDVSGFEETDVPGGTGAGDTGAYDELPDDALPISGG
                     YDPYGTGAAQTPAGFAEPTVAHSVGSFSESTASIEAAFGEQTASTQAPPFGADTPPSF
                     GTDTPKDGPQAGSPALGGGRADTSLEDDLDEAEFYASQRMFSEAIDLLSNLLTRFPNH
                     RLVLAKLREVEALAAGGEVAINVGSGGHPAAPGGTDALDLDEIEEMGPEELVELDANS
                     QPIQAPRKRQPTVMLEKPVDEGDVDTHYDLGLAYKEMALYDDAIKAFEQALRAPGREV
                     GCRVMIGMCLREQGNPSEAVHQFKQGLHAEPTERECQSLYYEIGITYEAIGDDTEALY
                     FFEMVLKRDPTFADAATRAERLRARGGRSAHVRDD"
     gene            complement(3425..4774)
                     /gene="accC"
                     /locus_tag="VF516_23810"
     CDS             complement(3425..4774)
                     /gene="accC"
                     /locus_tag="VF516_23810"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEX8110787.1"
                     /translation="MFKKVLIANRGEIALRVIRACREMGLASVAVYSTADADALHVKF
                     ADQAVCIGPPPSRQSYLNVPMIISAAEVTGADAVHPGYGFLSENADFAEVCRKCGLTW
                     IGPPADAMRLMGDKIRARAAMAEAGVPTLPGSSGLHDEVQLIEAAERIGLPVILKAAA
                     GGGGRGMKIVWEKEKLIEAWAMGRTEAQAAFGNPEMYMERYCAKPRHIEIQVVADKHG
                     HYTHLGERECSIQRRHQKVLEESPSAVLPEATRAKLTTASVRAIEAIGYTNVGTMEYL
                     LDSDGQFYFMEMNTRLQVEHPVTELVTGIDLVREQIRIAAGEPLSFTDERRARGHAIE
                     LRINAEDPNTFAPSPGKISALHMPGGLGVRVDTHVYAGYMVPPNYDSLLAKVIVHDVD
                     RMAALRRARRCLDEMVIEGIRTNIPFLRRIISNPEFIRGEFDTGFVGRLLAERASAP"
     gene            complement(4799..>5116)
                     /gene="accB"
                     /locus_tag="VF516_23815"
     CDS             complement(4799..>5116)
                     /gene="accB"
                     /locus_tag="VF516_23815"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEX8110788.1"
                     /translation="LPVASAPAPSPGGAAAAPAEAPAAADKHHIVTSPFVGTFYRRPN
                     PDSPNYVSLHDKVDKGQVLCIVEAMKLMNEIEADVAGTIVGILVEDGAPVEYGQPLFK
                     IAQ"
BASE COUNT          675 a         1751 c         1834 g          856 t
ORIGIN      
        1 gtcgtcgcgg acgtgggccg agcggccgcc gcgggcgcgc aaccgctcgg cgcgggtcgc
       61 ggcgtcggcg aacgtcgggt cgcgcttgag caccatctcg aagaagtaca gcgcctcggt
      121 gtcgtcgccg atcgcctcgt aggtgatgcc gatctcgtag tacaggctct ggcactcgcg
      181 ctcggtcggc tcggcgtgca ggccctgctt gaactggtgg accgcctcgg acgggttgcc
      241 ctgctcgcgc aggcacatgc cgatcatgac gcggcagccg acctcgcggc cgggcgcgcg
      301 cagcgcctgc tcgaacgcct tgatcgcgtc gtcgtacagc gccatctcct tgtaggccag
      361 gccgaggtcg tagtgggtgt cgacgtcgcc ctcgtcgacc ggcttctcga gcatcacggt
      421 gggctggcgc ttgcgcgggg cctggatcgg ctgcgagttg gcgtcgagct cgaccagctc
      481 ctcgggaccc atctcctcga tctcgtcgag atcgagcgcg tcggtgccgc ccggcgcggc
      541 cgggtggccg ccggagccga cgttgatcgc gacctcgccg ccggcggcga gcgcctcgac
      601 ctcgcgcagc ttggcgagca ccaggcggtg gttggggaac cgggtcagca ggttgctgag
      661 caggtcgatc gcctcgctga acatccgctg gctggcgtag aactcggcct cgtcgaggtc
      721 atcctccagg ctggtgtcgg cgcgtccgcc gccgagcgcc ggcgacccag cttgtggccc
      781 atctttgggg gtgtcggtgc cgaacgacgg cggcgtgtcg gcgccgaacg gcggggcctg
      841 cgtgctcgcg gtctgctcgc cgaacgccgc ctcgatgctg gcggtcgact cgctgaacga
      901 cccgacggag tgggcgacgg tcggctcggc gaaccccgcc ggggtctggg ccgccccggt
      961 gccgtacggg tcgtagccgc cggagatcgg cagcgcgtcg tcggggagct cgtcgtacgc
     1021 gccggtgtcg cccgcgccgg tcccgcccgg cacgtcggtc tcctcgaagc cgctgacgtc
     1081 gatcgcgtcg gtgccgcccg gctccatcgc gcggctgacc gcggcgacct ggctgctcgc
     1141 ccgcggcacc ccggcgtcga acgcgcgcgc ctcgtcgggg tcgaacggca ggtcgtcggc
     1201 gacgtcttcg gtgatgtcga cgggcttgcc ggcgccgcgc gcgagctcgc gggcgacgtg
     1261 gcgatcgatc tcgtcgccga gctcgatgtc ctcgagctgg gcgtcgagcg cgcgcgcgtc
     1321 gtcgagcgac tccgcccagc gcggccggtc gacgatcacc gggccgccca ggcggccacc
     1381 gaggggacca tcgagctcgt cggcgtgcga cgacgcgagc gcctcgacga tctcgggcga
     1441 catctggcgg gtcgcctgcg acggcgacgg ctcgaagtcg agctcgaagt tgtccgcctc
     1501 ggccgggtag ccgccgttgg ggcggcgcgg cggccggccg ggcgccgggc tgccgtgcgc
     1561 catcgcgccg atcagctcgt cgggatcgaa ctcgtcgatc gagtcccggc tcgatcggcc
     1621 ggcgtcgaac tcgccgtcgg cgagcgcgat cgcgccgtag tcggacgaca cctcgatgtc
     1681 ggggctcgcc gacgacatcc gcgccacccg cagccggaac ctgcgcgcga ggtcgtacgc
     1741 gccggtgtgc gtgccggaca gcgacagcag ctcctggagg tagagctcgg cgcggtcggg
     1801 gtcgctcggc gcggtcagct cggcgagccg catcagctcg gcctcggcct cggcctcgcg
     1861 gttctgcgac aggaagatgt ccttgagccg ctcgcgggcc tcgacgttgt cggcgtcgag
     1921 cgtgaacacc cggcgcaggt ggtcgatcgc cttctggtgc aggccgtact tcacgtagac
     1981 gtcggtctcg gcgaggatct tctggatctc ctcggcgtga cgctcgccgg ccgccgacat
     2041 cgcgcgcgga tcgggcggcg gctccatgtc ggccgagaag tcggcgtcgt cgtactcggg
     2101 cagcgcgaag tcgatgccgg acagcgactg ctcgtcgatc agcggcatcg agccggtcat
     2161 ccgcgccgac gcggcctcgt acggcggcat gtcgccggtc aggttgaacc gcgcatcgcc
     2221 ggcctgggcg cggatctgcg ccggcagcga cgtgaccgcg cgcggtatcg gggactgcga
     2281 cgggcgcgag ccggcctgcg cggacggccc gaggaacgcg atcgcgtccg gatcgccggg
     2341 caacagctcc aggatcttgc ggaacacctc gccggcgagc tccttgtgct tgttctcgac
     2401 gtggatccgc gcgagctcct tgagcacgct ggcggtcttg gccttctggt cgagcgcctg
     2461 gaacgcctgg gcgagcaggc cgagggtctc gacgtcgcgc ggatcggcct tgaagcaggc
     2521 ctggagcttc tgcagcgcgc gccgcggatc gttgcgccgc aggtacagcc cggcgagctc
     2581 gcggttgagc gcctggttgt tcggcttgtg ccacagcagg cgctcggcga ccttgaggaa
     2641 gtcgtcctgg cggccctggc ggcgcagctg ctcgcacgcg gtctggaact cggtcgccgc
     2701 gtcgtcgacc aggccctcct tggagtacag ctcggcgagc ttgatccggg tcgcgatgtt
     2761 ctcggggtcg atgtcgacca gcttcttgac cgcggcgagc gcctccttgg tgttgccctc
     2821 gcggtggaag tgcgcggcga ccgattcgaa gtgctgcatc gcctcgggca tcatccgcag
     2881 ctcgcggtag agctcggcga gcttcaagat cacgtcgacg aggcgcggat cgagcttgac
     2941 gatctgcttg tagaccgcga ccgacttatg caggaagccc tggtcgtgat agaagcgcgc
     3001 gaccttgagg taggtctcga tcgcgtcctg cttggcgccc ttcttggcgt agaggtcgcc
     3061 gatcttgagc cagacccgga cgtccttggg gtcctcgtgg acgatgcgca ggtactcctt
     3121 gaccgccttg tcgatctggc ccttgtcgac aaacttgcgc gcggcatcca tgaccttgtc
     3181 tttgttaaag gccacgggcc tccacaccct cccggtgcga agcatcacac caacgtgctt
     3241 ccacgcgggc catcaggtga cggcagtatg ccccgcgcat cggtcaaagg gtacggcctt
     3301 gcccgaggaa gggtcaagga aatccatgag agaatcccgg gcgttacccg accacgttcc
     3361 tcacatctga tcactggtcg gcgcgctcca tcgcacgggc cgatccccag ctcgtagtca
     3421 actgtcaggg cgctgacgcc cgctctgcga gcaatcgccc gacgaagccg gtgtcgaact
     3481 cgccgcggat gaactccggg ttggagatga tccgtcgcaa gaacgggatg ttggtccgaa
     3541 tcccctcgat gaccatctcg tcgaggcagc gccgggcgcg cctgagcgca gccatgcggt
     3601 cgacgtcgtg cacgatcacc ttcgcgagca gcgagtcata gtttggtgga accatgtaac
     3661 ccgcgtacac gtgcgtatct acacgcacac cgaggccgcc gggcatgtgg agcgcgctga
     3721 tcttgcctgg tgacggcgcg aacgtgttcg gatcctcggc gttgatccgg agctcgatgg
     3781 cgtgcccccg cgcgcggcgc tcgtcggtga atgacagcgg ctcgccggcg gcgatccgga
     3841 tctgctcgcg cacgaggtcg atgccggtga ccagctcggt gaccgggtgc tcgacctgca
     3901 gacgcgtgtt catctccatg aagtagaact ggccgtcgga gtcgagcagg tactccatcg
     3961 taccgacgtt ggtgtagccg atcgcctcga tcgcgcggac cgacgcggtg gtcagcttcg
     4021 cgcgggtcgc ctcggggagc accgccgacg gtgactcctc gagcaccttc tggtggcggc
     4081 gctggatcga gcactcgcgc tcgccgaggt gggtgtagtg gccgtgcttg tcggcgacga
     4141 cctggatctc gatgtggcgc ggcttggcgc agtaccgctc catgtacatc tcggggttgc
     4201 cgaacgcggc ctgggcctcg gtgcgcccca tcgcccaggc ctcgatcagc ttctccttct
     4261 cccagacgat cttcatgccg cggccgccgc cgcccgccgc cgccttgagg atcaccggga
     4321 gcccgatccg ctcggcggcc tcgatcagct ggacctcgtc gtgcagcccc gacgatccgg
     4381 gcagcgtcgg cacgccggcc tcggccatcg ccgcgcgcgc gcggatcttg tcgcccatca
     4441 ggcgcatcgc gtcggccggc gggccgatcc aggtcaagcc gcacttgcgg cagacctcgg
     4501 cgaagtcggc gttctccgac aggaagccgt agccggggtg gaccgcgtcg gcgccggtga
     4561 cctcggcggc cgagatgatc atcggcacgt tgaggtagct ctggcgcgac ggcggcgggc
     4621 cgatgcacac cgcctggtcg gcgaacttga cgtggagcgc gtcggcgtcg gcggtcgagt
     4681 acaccgcgac cgatgccagg cccatctcgc ggcacgcccg gatcacgcgc agcgcgatct
     4741 cgccgcggtt cgcgatcagt accttcttga acatggtcga gggctctttc gcctggcgtc
     4801 actgggcgat cttgaacagc ggctggccgt actcgaccgg cgcgccgtcc tcgacgagga
     4861 tcccgacgat cgtgccggcg acgtcggcct cgatctcgtt catcagcttc atcgcctcga
     4921 cgatgcacag cacctggccc ttgtcgacct tgtcgtgcag cgacacgtag ttcggcgagt
     4981 cggggttggg ccggcggtag aacgtgccga cgaacggcga ggtcacgatg tggtgcttgt
     5041 cggccgcggc gggggcctcg gccggcgcgg cggccgcccc gcctggagac ggggccgggg
     5101 cgctcgccac cggcag
//
LOCUS       DASVOR010001248         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_451007,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001248 DASVOR010000000
VERSION     DASVOR010001248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451007"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            433..897
                     /locus_tag="VF516_23820"
     CDS             433..897
                     /locus_tag="VF516_23820"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX8110789.1"
                     /translation="MTPEHPVTPVKENEPFRWLLRWDPFREMTPSWFGEPPVETTFFP
                     AFEVKENKESFLFKADLPGVKEQDVEIKLAGDRLTISGKREAEREDKNDTYYAYERSF
                     GSFLRTFTLPEGFDGDHVQAELKDGVLTVVVPKKAAAQAKTIAIKGADLRKS"
     gene            complement(929..1309)
                     /locus_tag="VF516_23825"
     CDS             complement(929..1309)
                     /locus_tag="VF516_23825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8110790.1"
                     /translation="MTRQPWTIEPSATLTAAHQLMRRHAIRHLPVMDAGKLVGIVTER
                     DLHLIETLPDADPDEVTVDDAMTEAVYVAAPTDEVSDVVDRMAADKLGSAVVMQGPRV
                     EGIFTSIDALQVLSHVLRRSVDPR"
BASE COUNT          278 a          479 c          639 g          215 t
ORIGIN      
        1 ccgcgcgccg gcgcagtcga tccgcgaagc ggtggagcag ctgatcgagg cgcaagaagc
       61 cgacgacgca gtcgcggagt ggatgcaggg cggcgggtga cggtgggcca agcggtgcgc
      121 gttcgcggac gggagcttcg caaaacaccg gggacaccgg ggacaccggg gacaccgggg
      181 acgccggaaa ccgcgttcga gatccgcgca cgcgacgtgc agtgacgcga gatcgcgagt
      241 cagcgtgagg cggctcgacg gcgatcggcg gagacgccga cgacgaaatt ccgcaatgga
      301 tgcgggcgaa aacggccgcc gccgcacgag ctgcgcgatg tgagcgcggc gagccggtgc
      361 gtggatggaa cactgattgc tgatcaaaga gcagtccctg gaaggaggac tgccatgtca
      421 ttgatccgtc gagtgacccc ggaacatccg gtaacgccgg tcaaggagaa tgaaccgttc
      481 cgctggctgc tgcgctggga tccgttccgc gagatgacgc cgtcgtggtt cggcgaaccg
      541 cccgtcgaga cgacgttctt cccggcgttc gaggtcaagg agaacaagga gagcttcctg
      601 ttcaaggccg acctgccggg cgtgaaggag caggacgtcg agatcaagct cgccggtgac
      661 cggctgacga tcagcggcaa gcgcgaggcc gagcgcgagg acaagaacga cacgtactat
      721 gcgtacgagc gctcgttcgg cagcttcctg cgcacgttca cgctgcccga agggttcgac
      781 ggcgaccacg tgcaggccga gctgaaggac ggcgtgctga cggtggtggt gccgaagaag
      841 gccgcggcgc aggccaagac gatcgcgatc aagggcgccg acctgaggaa gtcgtagccg
      901 cggccggtcc gcggcgccgc cggcgcggtc agcgcggatc gacggagcgg cgcaggacgt
      961 gggacaggac ctggagggcg tcgatcgagg tgaagatacc ctcgacgcgc gggccttgca
     1021 tcacgactgc cgagccgagc ttgtccgcgg ccatgcggtc gacgacgtcg gagacctcgt
     1081 cggtcggtgc ggcgacgtag accgcctcgg tcatcgcgtc gtccacggtg acctcgtcgg
     1141 gatcggcgtc gggcaaggtc tcgatcaggt ggagatcgcg ctcggtgacg atgccgacca
     1201 gcttgccggc gtccatgacc gggaggtggc ggatcgcgtg gcggcgcatg agctggtggg
     1261 cggcggtcag cgtggcgctg ggctcgatgg tccacggctg ccgcgtcatg tagcggctga
     1321 tcggcggcat caacatgtct ggaggaggag cagaagccgt gccacggctg cgacgggaga
     1381 tcgcgcagcg gatgcggccc accggcgcgg cggcgcaagc ggatcgcggt ggggcgctgc
     1441 acgggagccg ggtgaaccgc cggggacttg gccgggtgga acgccggttg ctccctgtgg
     1501 ggccatgaag cgcgtcatcg tcgcgggagg tgtgctggcc gcgtgcgcgg tgatcgccgc
     1561 cggcgctgcg tggcggaccc ggcggactga ggcgccggag tccccggcga g
//
LOCUS       DASVOR010001249         3588 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_392233,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001249 DASVOR010000000
VERSION     DASVOR010001249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392233"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..307)
                     /locus_tag="VF516_23830"
     CDS             complement(<1..307)
                     /locus_tag="VF516_23830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110791.1"
                     /translation="MSLDALIDAIRAADGEAGPGGAADDAELAVATRLRVMRSLEARA
                     RSRHQLIGFATAVAILLGGTVSWALATGQVAAIWTPAPAPQPQPQPAVEPAIAPPRPV
                     "
     gene            complement(304..1020)
                     /locus_tag="VF516_23835"
     CDS             complement(304..1020)
                     /locus_tag="VF516_23835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8110792.1"
                     /translation="MTAIPMPLMMPAVPEVPAAPDARSVNDVQDDEAVIEADIEADIE
                     ADIDADIDAAIDADADLAARLRAGDRAAVERVYVAHHAAIRGFARRLVGDAASAEDIV
                     HETFVALPRAIRRFRGEGSLRAFLIGVAANHSRRHVRSAMRRRHATERLAAREEVQPR
                     AVDATAALINRQMAHRLWAALDELPIDQRVVFVLCEAEQRTSVEVAAIVDAPEGTVRT
                     RLFHAKRKLRQMLGAEEEAP"
     gene            1257..1781
                     /locus_tag="VF516_23840"
     CDS             1257..1781
                     /locus_tag="VF516_23840"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HEX8110793.1"
                     /translation="MPLRVLPPASPDAGCPGCSRRAVLHGLAVTAASVLVGCPSSDGS
                     MSGTGPDAGEGGDGGSGSTASACGANLCVDLNDPKNAALTAVDGALVVAAPRDSILLV
                     RSSATVVAAVSDICTHAGCGVRYDHVNKVLSCPCHGSKYTLTGMVLQGPAFRPLANYQ
                     TQLDPGTNLLTILL"
     gene            2230..3381
                     /locus_tag="VF516_23845"
     CDS             2230..3381
                     /locus_tag="VF516_23845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110794.1"
                     /translation="MGRVLPFRLRTDLGVVNPPPVIVIADRDAETRETVRGFMSARGY
                     RVEPASDVDTAAGLLRSRNIDVLITELALRGPDGTDLLSLAHASAPATRRIAIAADAT
                     VRERDAALRLGVVRVLAKPLSLLELADAIGLAHDCAEGLHGWMHRMSLVDVLQMYHHA
                     GQSLVIHVNGDVEGAIALRHGELIHAECHGRVGMPALVELLTVQRGQLETTALDHASR
                     TITGPFDHVLLDGLRSLDEVRRPAGPAAAPAAADDWLDDSAMRGAPDREALQRWLGEH
                     APDAGAWQIDPVALTIERIDPLGPHPERELAGPPNSLGWACELAELADPAWTRAELLA
                     GDCAIAVFRVSTVVIAFARVVAGDAMLRRFQVESARLMRWLTEHPGSAP"
     gene            3378..>3588
                     /locus_tag="VF516_23850"
     CDS             3378..>3588
                     /locus_tag="VF516_23850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110795.1"
                     /translation="MRSRVSLFETTVVDGGALGAAAIDVATRAVVAGYRHDGLASPAL
                     LDLLLGGVRPQGPLTRLCGGPRPGAA"
BASE COUNT          470 a         1331 c         1254 g          533 t
ORIGIN      
        1 gcaccggccg cggcggcgcg atcgccggct cgaccgccgg ctgcggctgc ggctgcggcg
       61 ccggtgcagg cgtccagatt gccgcgacct gaccggtcgc gagcgcccac gataccgtgc
      121 cgccgagcag gatcgcgacc gcggtcgcga acccgatcag ctgatgccgg ctgcgcgcgc
      181 gggcctccag cgagcgcatc acgcgcagcc gggtcgccac cgcgagctct gcgtcgtcgg
      241 cggccccgcc cggcccggcc tcgccgtcgg ccgcgcggat cgcgtcgatc agtgcgtcga
      301 ggctcacggc gcttcctcct ccgcccccag catctggcgc agcttgcgct tggcgtggaa
      361 cagccgcgtc cgcaccgtgc cctccggtgc gtcgacgatc gcggcgacct cgaccgacgt
      421 ccgctgctcg gcctcgcaca gcacgaacac gacccgctga tcgatcggca gctcgtcgag
      481 cgccgcccac agccggtgcg ccatctgccg gttgatcagc gcggcggtcg cgtcgaccgc
      541 ccggggctgg acctcctcgc gcgccgccag ccgctcggtc gcgtgccggc gccgcatcgc
      601 cgaccggacg tggcgccgcg agtggttggc cgcgacgccg atcaggaacg cccgcagcga
      661 gccctcgccg cgaaaccgcc ggatcgcgcg cggcagggcg acgaacgtct cgtgcacgat
      721 gtcctcggcc gacgcggcgt ctccgaccag ccggcgcgcg aacccgcgga tcgccgcgtg
      781 gtgcgcgacg tagaccctct cgaccgccgc gcgatcgccc gcgcgcagcc gcgctgcgag
      841 atcggcatcg gcgtcgatgg ccgcatcgat gtccgcatcg atgtccgcct cgatgtccgc
      901 ctcgatgtcc gcctcgatga ccgcctcgtc gtcctgcacg tcgttcacgc tccgcgcgtc
      961 cggagccgcc ggcacctcgg gcacggccgg catcatcagc ggcatcggga tcgcggtcac
     1021 cagctcatgg tgcccggtcc ttgcgcaatc gttcaatgcg atcgtctcgc ccgccaaccg
     1081 gctggaatga ttgacggaac tcccgatgcc gcgaccggcg ttcgcgcccg atgccggctc
     1141 gcgcacggcc ggtacagcgc cggtgccgct gcggacagcc ccggacagtg atcggcgcga
     1201 cgcgtgcaat tctcgtcggt ggacaccgcc gcgatgggcg gcaaccatga ccgcgcatgc
     1261 cgctgcgcgt cctcccgccc gcctcgcccg acgcaggatg cccgggttgc tcgcgccgcg
     1321 cggtgctcca cggcctcgcg gtgaccgccg cgagcgtgct ggtcggctgc ccgagcagcg
     1381 acggctcgat gagcggcacc ggacccgacg ccggcgaagg cggcgacggc ggatcgggtt
     1441 cgaccgcgtc ggcgtgcggc gccaacctgt gcgtcgatct caacgatccg aagaacgccg
     1501 cgctgaccgc ggtcgacggt gcgctggtcg tcgccgcgcc cagggactcg atcctcctcg
     1561 tgcggtcctc ggccaccgtc gtggcggccg tctcggacat ctgcacccac gccgggtgcg
     1621 gcgtgcgcta cgaccacgtc aacaaggtac tcagctgtcc gtgccacggg tcgaagtaca
     1681 cactcaccgg aatggtgctc caggggcctg ctttccgtcc actagcgaat tatcagacac
     1741 agctggatcc aggcacgaat ctcttgacga tcttgctgta gtcacggcta tggagttatg
     1801 gcggcggcgt ggcgcgtctg cgccaaagac ctacgccaag aacgtaagga ttagcggcac
     1861 gttagtacgg ccagctaccc caccgttacc ccaaggaaga gagtgggtaa tcttccgctg
     1921 cagttgaggg atcggcatcc tgcttcctga tggggcctca tgcggagtac ctgcgcgttt
     1981 ctcatctgat cgaaagggca gcgattcacg gcgtcacgat gtgccggcat cttgctatgt
     2041 gcctcacagc ggcgcgctgt tgtgctgcga tccgcggcgc gacgattcgc ttgtcccaga
     2101 cgcaaaccgc cgcactcgaa cctcgtcggc gcctcacgca gcgtacacag cagatactcc
     2161 gcatccgtga gcgcgtaccc gtgccggcgc gccgcacgca ccactcgctg gagtgggggt
     2221 cgtgttgacg tgggccgcgt cctgcccttt cgcctccgga cggatctcgg cgtcgtcaac
     2281 ccgccgccgg tgatcgtcat cgccgatcgc gatgccgaga cacgcgagac cgtccgcggc
     2341 ttcatgtcgg cgcgcggcta ccgggtcgag cccgcctccg acgtcgatac cgccgcgggt
     2401 ctgttgcgca gccgcaacat cgacgtcctg atcacggagc tcgcgctgcg cggcccggac
     2461 ggcaccgacc tgctgtcgct ggcccacgcg tcggcgcccg cgacccggcg gatcgcgatc
     2521 gcggccgatg cgaccgtgcg cgagcgcgac gcggcgctcc ggctcggcgt ggtgcgggtg
     2581 ctcgccaagc cgctgtcgct gctggagctc gccgacgcga tcggcctggc ccacgactgc
     2641 gcggagggcc tgcacggctg gatgcatcgg atgtcgctgg tcgacgtgtt gcagatgtac
     2701 catcacgcgg gtcagtcgct ggtgatccac gtgaatggcg acgtcgaggg cgcgatcgcg
     2761 ctgcgccacg gcgagctgat ccatgccgag tgccacggcc gggtcgggat gccggcgctg
     2821 gtcgagttgc tcaccgtgca gcgcggacag ctggagacca ccgcgctgga ccacgcgagc
     2881 cggacgatca ccggcccgtt cgatcacgtg ctgctcgatg ggctgcgctc gctcgacgag
     2941 gtccgccgcc cggccggccc tgccgctgcc cccgccgcgg ctgacgactg gctcgatgac
     3001 agcgccatgc gcggcgcgcc cgatcgcgag gcgctgcagc gctggctcgg cgagcacgca
     3061 ccggacgccg gagcgtggca gatcgacccg gtggcgctga cgatcgagcg gatcgatcca
     3121 ctcgggccgc atcccgagcg cgagctcgcg gggccgccga actcgctggg ctgggcgtgc
     3181 gagctcgccg agctcgccga tccggcgtgg acccgcgccg agctgctcgc cggtgactgc
     3241 gcgatcgccg tgttccgggt atccacggtc gtgatcgcgt tcgcgcgcgt ggtcgcgggc
     3301 gacgcgatgc tgcgccggtt ccaggtcgag agcgcgcggc tgatgcgctg gctgaccgaa
     3361 caccccggga gcgcgccgtg aggtcgcgcg tctcgctgtt cgagaccacc gtcgtcgacg
     3421 gcggagcgct cggtgccgcc gcgatcgacg tcgcgaccag ggcggtggtc gcgggctacc
     3481 gccacgatgg cctggcgagc cccgcgctgc tcgatctgct gctcggcggc gtgcggccgc
     3541 aggggccgct gacccggcta tgcggcggtc cgcggcccgg cgcggctc
//
LOCUS       DASVOR010001250         6514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_217403,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001250 DASVOR010000000
VERSION     DASVOR010001250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217403"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            422..1045
                     /locus_tag="VF516_23855"
     CDS             422..1045
                     /locus_tag="VF516_23855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110796.1"
                     /translation="MVRHGAEAKRGRTGSRRSDAEKRRQTGEHKRRDAERRRVLAEQL
                     RKLASRALDLTEAARKAAEEARHHRDEIVRTANALERKVEHERARAEQLRTEAERLRE
                     GSELRRQRTEAFRVRAELGRASFEETRQLREDIRRQSEGLRASSELERKQESRATSGG
                     ETSPATEEISPALRQAIRDEVRSALAEARSVAATGGEKLPMSRRSDR"
     gene            complement(1062..1916)
                     /locus_tag="VF516_23860"
     CDS             complement(1062..1916)
                     /locus_tag="VF516_23860"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8110797.1"
                     /translation="MPIRGYYLGCPGWGVKTWVGRLFPTGTRPTEFLERYARVFNTVE
                     GNTTFYALPTADVVARWRDQVPDDFRFCFKFPREVSHDKLLIDCGAELAAFLDRVAPL
                     GDKLGTLFLQLPPRFDPALLPRLRGFLAALPAGPRYAVEVRHDAFFGDGPAQDELLAL
                     LRERGADLVTMDTRGLHTGKSLALADVRARKPNLPVHVRATADRPIVRCVPHEQFDAA
                     RPFVEPWAAQIARRIAEGKRPYFFMHTPDDTFAPENAYAFHAMVRRHADVGELPAWPG
                     GERQLSLL"
     gene            complement(1962..2849)
                     /locus_tag="VF516_23865"
     CDS             complement(1962..2849)
                     /locus_tag="VF516_23865"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8110798.1"
                     /translation="MERKQASDFDQELLNLFDRYVHGDIDRRGFLDRAAKYAVGGVTA
                     LMLLDQLNPRFAEAEVVPKDDKRLTTSTVEIDSPKGTGKVKAYFARPASAKGKLPGVL
                     VIHENRGLNPHIEDIARRIALEGFDALAPDALTVSGGYPGDEDKAREAFGKLDQDKAR
                     QDFLAAAAALKARPDCTGKIGAVGFCWGGGMTNFLATQLPDLAAAVPFYGAQPKADDV
                     AKIKAPMLIHYAGNDDRINAGIPAFEDALKASKVKYQLFKYDGTQHGFNNDTTPRFDK
                     TAAALAWKRTVDFFKKSLK"
     gene            complement(3001..5262)
                     /locus_tag="VF516_23870"
     CDS             complement(3001..5262)
                     /locus_tag="VF516_23870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110799.1"
                     /translation="MMRQRFLLASACVGAAALSGAILALHGCSGDGTGDLGQPAVICG
                     SGTTGAACTDGTTTPISALPFVDAASNAQLLGELLAKPTALGETTALQVRLPPPTNQS
                     VAGSLVRVIGDVNEPHVVFSSDALVRLGLLGKSPGPDFFTAFATLTSDELSKVQSNQR
                     QIASGVFGQTTSESVLFASRAATARTSFPAVDPAQFLPGNLIPVNRCPSRPVSTLQAW
                     GQSLFITSPQVVLDPARTWDPCTGAGTQGGVWTFAHLMREMANGSGKTPEDFVKDWLS
                     LWLNNYTVNGDTVPARTQMFNQVIQPWATASGVTATLVIDPVTGVRSVNLTGPLNLNI
                     APYRLLAIVNRIDLGTTTHGGGGYGSVTGTPVTAGELRFIFGVVQPNPWGAGTDATCG
                     KKFFTTIFEYGVPRTGCAAVVNWAQQWSSLQTFPGFTAAYLAQLQSMTESVVLHGDAP
                     AKGNQNALDQIRTNEIALAGPWELREFTLTNEQPAANIDPPANGELRTHTVAQTPDDA
                     RFSPDGADPTVNAFVAGPVTVGLQLPVVLPPTVPSRCDSSYTVPFIFNNLQFRGGNAL
                     VQAPPHPPGAEFWKANSIPATPNGICARHIFSLNTCHGCHRGDSGNRSTLGSIDFTHV
                     TPTSTIPVTLSKFLTGGGPGMTFNVNDTQFGAPQWPFADLDRRLQRLFELSHCTSCIT
                     LFPMTPKLVDTMQTFGPVPADVNPSDFPDLKIGPITDLTTLQRVIDLRAQVAGASRNE
                     PVDVIRSIDNSVH"
     gene            5649..6101
                     /locus_tag="VF516_23875"
     CDS             5649..6101
                     /locus_tag="VF516_23875"
                     /inference="COORDINATES: protein motif:HMM:NF024844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEX8110800.1"
                     /translation="MKLALTIGELDSRVGQEVAVSPWVEITQDRIDEFAKATGDFQWI
                     HVDRERARQSPFGGTIAHGFLTLSLLPMLTEAAYTLTDRSMGINYGLNKVRFTSPVPA
                     GSKIRARFTLTKLDRLDGGVQTTWSVVVEREGSDKPVMVAETLGRHYR"
     gene            complement(6172..>6514)
                     /locus_tag="VF516_23880"
     CDS             complement(6172..>6514)
                     /locus_tag="VF516_23880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110801.1"
                     /translation="KAAPRGRPPVHVVDADRLIAWQLYWRGENFWSGDEIWGYPPEMK
                     TAFKEANNVAFTKYINDRARAPLGRRYFLITEAGRITAPRASLPTQRARDSYEVLDTT
                     SNKFSLAAFWL"
BASE COUNT          985 a         2104 c         2460 g          965 t
ORIGIN      
        1 cgttgccgtg ccaccagcgc gacgcggtcg acgagccgcc gagccgtggc accgagtcgc
       61 gaaatagacg ccaccgctgc gctcgaccca ctccacgaga agccgcgtct tgccgacacg
      121 gcgccggccg gtgaccaccg ccaggcccct tcgcgccgct tcgagagcgc gagcaagcgc
      181 gacagctcgt cttgacggtc gaggaacttc ctatgcagga ccgcatcatg ccatccggca
      241 taagaagcga taacccccgc ccggcgtcgc tcaggcttcg agcgtccgcg cgatcagtca
      301 ggggacgtcg gaggagcatc gtcgccgccc cgttcgccac gctgaagcgg ccctggctcg
      361 tggaaaccaa cagtgacaca gtccggtcat gcctcctgct cgcccggccg gaacgaaacg
      421 cgtggttcgc cacggcgcgg aggccaagcg agggcgcacc ggatcacggc gatcggacgc
      481 cgaaaaaaga cgtcagacag gggagcacaa acgccgcgac gccgaacgca ggcgtgtcct
      541 cgccgagcaa ctgcgcaagc tcgcgtcgag ggcactggat ctaaccgagg cggcccggaa
      601 agcggccgaa gaagctcgcc atcatcgcga cgagattgtc aggacggcca acgcgctcga
      661 gcgcaaggta gagcatgagc gcgctcgggc ggagcagctg cggacagaag ccgaacgcct
      721 gcgcgagggc tcggagctca ggcgccagcg cacggaagcc ttcagggtcc gtgccgagct
      781 tggacgcgct tcgttcgaag aaacgcgcca gcttcgagaa gatatccggc gacaatcgga
      841 aggcctacgc gccagctcgg agctggagcg caagcaggag tcgcgcgcca cgagtggtgg
      901 cgagaccagt cccgccacgg aagagatcag tcccgcgctc cgccaagcca tccgcgatga
      961 agtgcggagc gcgctggctg aggcgaggtc ggtggcagct accggcggcg aaaagctgcc
     1021 catgtcgcgg cgctccgacc gctagcctcg cgcgcgcctc gctacagcag gctgagctgg
     1081 cgctcgccgc cgggccaggc gggcagctcg ccgacgtcgg cgtggcggcg gaccatggcg
     1141 tggaacgcat aggcgttctc gggggcgaag gtgtcgtcgg gggtgtgcat gaagaagtac
     1201 ggccgcttgc cctcggcgat ccggcgcgcg atctgggccg cccacggctc gacgaacggg
     1261 cgcgcggcgt cgaactgctc gtgcggcacg cagcgcacga tcgggcggtc ggcggtcgcg
     1321 cggacgtgga ccggcaggtt cggcttgcgg gcgcggacgt cggcgagcgc gaggctcttg
     1381 ccggtgtgca ggccgcgggt gtccatggtc acgaggtcgg caccgcgctc gcggagcagg
     1441 gcgagcagct cgtcctgggc cgggccgtcg ccgaagaacg cgtcgtggcg gacctcgacg
     1501 gcgtagcgcg ggccggcggg gagggcggcg aggaacccgc gcagccgggg cagcagcgcg
     1561 gggtcgaacc gcggcggcag ctggaggaac agcgtgccga gcttgtcgcc gagcggcgcg
     1621 acccggtcga ggaaggccgc gagctcggcg ccgcagtcga tcagcagctt gtcgtggctg
     1681 acctcgcgcg ggaacttgaa gcagaaccgg aagtcgtcgg ggacctgatc gcgccagcgc
     1741 gcgacgacgt cggcggtggg gagcgcgtag aacgtggtgt tgccctcgac ggtgttgaac
     1801 acccgggcgt agcgctcgag gaactcggtc ggccgcgtcc cggtcgggaa cagccggccg
     1861 acccaggtct tgacccccca gccggggcag ccgaggtagt agccgcggat cggcatggct
     1921 gcgttgtggc gcgcggccgg gcgcgttgca agcgccggcg gctacttgag gctcttcttg
     1981 aagaagtcga ccgtgcgctt ccaggccagc gcggcggcgg tcttgtcgaa ccgcggcgtg
     2041 gtgtcgttgt tgaagccgtg ctgggtgccg tcgtacttga acagctggta cttgaccttg
     2101 ctggccttga gcgcgtcctc gaacgcgggg atcccggcgt tgatccggtc gtcgttgccg
     2161 gcgtagtgga tcagcatcgg cgccttgatc ttggcgacgt cgtcggcctt gggctgggcg
     2221 ccgtagaacg ggaccgcggc ggcgaggtcg ggcagctggg tcgcgaggaa gttggtcatc
     2281 ccgccgcccc agcagaaccc gacggcgccg atcttgccgg tgcagtccgg gcgcgccttg
     2341 agcgcggcgg cggcggcgag gaagtcctgg cgcgccttgt cctgatcgag cttgccgaac
     2401 gcctcgcgcg ccttgtcctc gtcgccgggg tagccgccgg agacggtcag cgcgtcgggc
     2461 gcgagcgcgt cgaagccctc gagcgcgatc cgccgcgcga tgtcctcgat gtgcgggttg
     2521 agcccgcggt tctcgtggat caccagcacg ccgggcagct tgcccttggc ggacgccggc
     2581 cgcgcgaagt acgccttgac cttgccggtg cccttgggcg agtcgatctc gacggtgctg
     2641 gtggtcagcc gcttgtcgtc cttgggcacg acctcggcct cggcgaaccg cgggttgagc
     2701 tggtcgagca gcatcagcgc ggtcacgccg ccgaccgcgt acttggcggc gcggtcgagg
     2761 aagccgcggc ggtcgatgtc gccgtggacg tacctgtcga acagattcaa gagctcctga
     2821 tcgaagtcgc tcgcttgctt gcgttccatg ggggggccct ccggtggcgc gcagaatatc
     2881 cgcggcgcgg ggcggcgcaa acccgccggg cggccgactc gcggcgacgc gccgggagtg
     2941 gcgaaccgcc cccgccggcc ggtccaggtc agccggcacg gagctgccag ggcgcgcggt
     3001 ctagtgcacc gagttgtcga tcgagcggat cacgtcgacc ggctcgttgc gcgacgcgcc
     3061 ggcgacctgg gcgcgcaggt cgatgacccg ctgcaacgtc gtcagatcgg tgatcgggcc
     3121 gatcttgaga tcggggaagt cgctcgggtt cacgtcggcg gggaccgggc cgaacgtctg
     3181 catcgtgtcg accagcttgg gcgtcatcgg gaacagcgtg atgcacgacg tgcagtgcga
     3241 cagctcgaac agccgctgca gccggcggtc gaggtcggcg aacggccact gcggcgcgcc
     3301 gaactgggtg tcgttgacgt tgaacgtcat gccggggcca ccgccggtca ggaacttcga
     3361 cagcgtgacc gggatcgtgc tcgtcggcgt gacgtgggtg aagtcgatgc tccccagcgt
     3421 gctccggttg ccgctgtcgc cgcggtggca gccgtggcag gtgttgagcg agaagatgtg
     3481 tcgcgcgcag atgccgttcg gggtcgcggg gatgctgttt gctttccaga actcggcgcc
     3541 cggggggtgc ggtggcgcct ggaccagcgc gttgccgccg cggaactgga ggttgttgaa
     3601 gatgaacggc acggtgtacg acgagtcgca cctgctcggc accgtcggcg gcagcaccac
     3661 cggcagctgg aggccgacgg tcaccgggcc ggcgacgaag gcgttgacgg tggggtcggc
     3721 gccgtccggg ctgaaccggg cgtcgtcggg ggtctgcgcg acggtgtggg tgcggagctc
     3781 gccgttggcg ggcggatcga tgttggccgc cggctgctcg ttggtcagcg tgaactcgcg
     3841 cagctcccac gggccggcga gcgcgatctc gttggtgcgg atctggtcga gcgcgttctg
     3901 gttgcccttg gccggcgcgt cgccgtgcag cacgacgctc tcggtcatcg actggagctg
     3961 cgcgaggtac gcggcggtga agccggggaa ggtctgcagc gagctccact gctgcgccca
     4021 gttgacgacc gccgcgcagc cggtgcgggg cacgccgtac tcgaagatcg tggtgaaaaa
     4081 cttcttgccg caggtcgcgt cggtgccggc gccccacggg ttgggctgga cgacaccgaa
     4141 gatgaagcgc agctcaccgg cggtcaccgg cgtgccggtc acgctgccgt agccgccccc
     4201 gccgtgggtc gtggtgccga ggtcgatgcg gttgacgatc gcgagcagcc ggtacggcgc
     4261 gatgttgagg ttgagcgggc cggtgaggtt gacgctgcgc acgccggtga ccggatcgat
     4321 gaccagcgtg gcggtcacgc cgctggcggt ggcccacggc tggatcacct ggttgaacat
     4381 ctgggtccgc gccgggacgg tgtcgccgtt gaccgtgtag ttgttgagcc acagcgacag
     4441 ccagtccttg acgaagtcct cgggagtctt gcccgagccg ttggccatct cgcgcatcag
     4501 gtgggcgaac gtccagaccc cgccctgggt gcccgcgccg gtgcacgggt cccaggtccg
     4561 cgccgggtcg agcacgacct gcggcgaggt gatgaacagc gactggcccc aggcctgcag
     4621 cgtcgacacc gggcggctcg ggcagcggtt gaccgggatc aggttgcccg gcaggaactg
     4681 cgccgggtcg accgccggga agctggtgcg cgcggtcgcg gcgcggctcg cgaacagcac
     4741 cgactcggac gtggtctggc cgaacacgcc cgacgcgatc tggcgctggt tgctctgcac
     4801 cttgctgagc tcgtcggagg tcagcgtcgc gaacgcggtg aagaagtcgg gcccggggct
     4861 cttgccgagc aggccgagcc ggaccagcgc gtccgagctg aacaccacgt ggggttcgtt
     4921 gacgtcgccg atcacgcgga ccagcgagcc ggccacgctc tggttggtcg gcggcggcag
     4981 ccggacctgc agcgcggtgg tctcgccgag cgcggtcggc ttggcgagca gctcgccgag
     5041 cagctgcgcg ttgctggcgg cgtcgacgaa tggcagcgcc gagatcggcg tggtggtgcc
     5101 gtcggtgcag gcggcgccgg tggtgccgct gccgcagatc accgcgggct ggccgagatc
     5161 gccggtgccg tcgccggaac agccgtggag cgcgaggatc gccccggaca gggccgcggc
     5221 acccacgcag gccgacgcga gcaggaatcg ctgtctcatc attcagttac tccttcattg
     5281 agatcggttg ggccggcgag actagatacc tcgctggcac ccaatgaagg ctgcccgcgg
     5341 cgatgatcgc cggggtcgcc cggcccggcc cggccccggg gacgctccgt cgagatcgcg
     5401 cgagtcgacg taccttggat gaaacagcga gtgtagagtc agcgtgcata ctggtgcggc
     5461 gcgcggtccg gcgtcaacat cagatcttaa ttctcgacaa tcagccgcaa tatgcggcac
     5521 agagatgtgc gtgttcgccg agcaatgatt gcgatcatcg agaatcgcca cggagttcat
     5581 gaggacgagc tatccgcgtc gcgctcccgg gctggcggcg cgccacccca ggcgcgtaga
     5641 atcaggtcat gaagctcgcg ctcacgatcg gagagctgga cagccgcgtc ggccaggagg
     5701 tcgccgtgtc accctgggtc gagatcacgc aggaccggat cgacgagttc gcgaaggcga
     5761 ccggcgactt ccagtggatc cacgtcgacc gggagcgggc caggcaatcg ccgttcggcg
     5821 gcacgatcgc gcacggcttc ctcacgctgt cgctgctgcc gatgctgacc gaggccgcgt
     5881 acacgctgac cgaccggtcg atggggatca actacgggct gaataaggtg cggttcacgt
     5941 cgccggtccc ggccggatcg aagatccgcg cccggttcac gctgaccaag ctggacaggc
     6001 tggacggcgg tgtccagacc acctggagcg tggtggtcga gcgcgagggc agcgacaagc
     6061 cggtcatggt cgccgagacc ctcggccggc actaccgcta gcgcggaccg agcgaagccg
     6121 ggcctcgggc tcctgcccga gaccccgaaa cagcgaagcg agggacgcgc gtcacagcca
     6181 gaacgcggcg agcgagaact tgttgctcgt ggtgtcgagc acctcgtagc tgtcgcgggc
     6241 gcgctgggtc ggcaggctgg cgcgcggcgc ggtgatccgg ccggcctcgg tgatcaggaa
     6301 gtagcggcgg ccgagcggcg cgcgggcgcg gtcgttgatg tacttggtga acgcgacgtt
     6361 gtttgcttcc ttgaacgcgg tcttcatctc cggcgggtag ccccagatct cgtcgccgga
     6421 ccagaagttc tcgccgcgcc agtacagctg ccacgcgatc agccggtcgg cgtcgaccac
     6481 gtgcaccggg ggccggccgc gcggcgccgc cttg
//
LOCUS       DASVOR010001251         1620 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363434,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001251 DASVOR010000000
VERSION     DASVOR010001251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1620)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1620)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1620
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363434"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1067)
                     /locus_tag="VF516_23885"
     CDS             complement(<1..1067)
                     /locus_tag="VF516_23885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110802.1"
                     /translation="MSSTPAQKFVCPRCRTSFDQPCRFCAECGADMTHASPLEAAQRA
                     EASAGPATGLLSAGRDLDPVDRRLSDSNRAWLGKVVDGRYRVLEVIGRGGMGVVYRVE
                     HLRMGKIAAMKVLHRDLAHDPEIVQRFEREAAAISKLHHPHTVQVFDFGTAQGALYLI
                     MEYVHGLDLAHIVQRDGPIPWPRCAPLLAQICGALQEAHDLGIVHRDLKPENVLITRT
                     TGGRDYAKVLDFGLAKLDPRGAEAREPVRETERNQIVGTPYYMAPEQIRGDEVGPRAD
                     IYAFGSLMFELLTGQHLYTGSTAVGVLTKHLTAEPDAPSARAPRLGIPPAVDQLCRKA
                     LARDRDARWQSAGELAEAIEEV"
     gene            complement(1427..>1620)
                     /locus_tag="VF516_23890"
     CDS             complement(1427..>1620)
                     /locus_tag="VF516_23890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110803.1"
                     /translation="LDTDDVVEVGVLLGAAHVAFDETEPALVCFKRALDRQAQHALRR
                     YDYSPKVLAVWQKAGGPIE"
BASE COUNT          231 a          557 c          578 g          254 t
ORIGIN      
        1 acctcctcga tcgcctcggc gagctcgccg gcggattgcc atcgcgcgtc gcgatcgcgg
       61 gccagcgcct tgcggcacag ctggtcgacc gcgggcggga tcccgagccg cggggcgcgc
      121 gccgacggcg cgtcgggctc ggcggtcagg tgcttggtca gcacgccgac cgcggtcgag
      181 ccggtgtaga ggtgctggcc ggtgagcagc tcgaacatca gcgagccgaa cgcgtagatg
      241 tcggcgcgcg ggccgacctc gtcgccgcgg atctgctcgg gcgccatgta atacggcgtg
      301 ccgacgatct ggttgcgctc ggtctcgcgc accggctcgc gcgcctctgc gccgcgcggg
      361 tcgagcttgg ccagcccgaa gtcgagcacc ttggcgtagt cgcggccgcc ggtggtgcgc
      421 gtgatcagca cgttctccgg cttgagatcg cggtgcacga tgccgaggtc gtgggcctcc
      481 tgcagcgcgc cgcagatctg ggcgagcagc ggggcgcagc gcggccacgg gatcggcccg
      541 tcgcgctgga cgatgtgcgc gagatcgagg ccgtgcacgt actccatgat caggtacagc
      601 gcgccctggg cggtcccgaa atcgaacacc tgcacggtgt gcgggtgatg caatttgctg
      661 atcgcggcgg cctcgcgctc gaaccgctgc acgatctcgg ggtcgtgcgc gaggtcgcgg
      721 tgcagcacct tcatcgccgc gatcttgccc atccggaggt gctcgacccg gtagaccacg
      781 cccatgccgc cgcgcccgat cacctcgagc acgcggtagc ggccgtcgac gaccttgccc
      841 agccacgcgc ggttcgagtc gctgagccgg cggtcgaccg ggtcgagatc gcggccggcg
      901 gacaaaaggc cggtggcagg gccggccgac gcctcggcgc gctgcgcggc ctccagcggg
      961 ctggcgtggg tcatgtcggc gccgcactcg gcgcagaacc ggcacggctg gtcgaacgat
     1021 gtccggcacc gcgggcagac gaacttttgc gcgggcgtcg aggacatcgc cgccccgagc
     1081 atagcaccgc gatctcgcgc ggcagggcgg agccccggtg gccccgctca agaggtgtgg
     1141 cttgaaggcg cagccgtccg tcatgacctt gacgatgacc cagaccttga ccctggcgtt
     1201 gaggtcgacg gcgacgttgg acctggacac caccgtcaac ctcgacgtga actcccgggg
     1261 tgccttctcg acgagattct cgtcggcaat gctgggtcca cgttcaacgt ccacggtgga
     1321 gtcagacgtc gacgttgccg tcaagctcaa cgctttgcgt cgacgtcgaa gtcaacctca
     1381 acgtctgcgg gacccaggtc cgcttctttg atctacagct cttcattcac tcgatcggcc
     1441 cgccggcctt ctgccacacc gcgaggacct tcggcgagta gtcgtagcgc cgcagcgcgt
     1501 gctgggcctg gcgatcgagc gcgcgcttga agcacaccag cgcgggctcg gtctcgtcga
     1561 acgcgacgtg ggcggcgccg agcagcacgc cgacctcgac gacgtcgtcg gtgtcgaggt
//
LOCUS       DASVOR010001252         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_217446,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001252 DASVOR010000000
VERSION     DASVOR010001252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_217446"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1885
                     /locus_tag="VF516_23895"
     CDS             <1..1885
                     /locus_tag="VF516_23895"
                     /inference="COORDINATES: protein motif:HMM:NF025869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HEX8110804.1"
                     /translation="PALDRTLLAIWNCLESLGSHPVLLTVPGNHDLVRPPAGDPRLAE
                     LARWPGDPAVAQRFWAEPASLQRALVTEAFAPYTRWWSDHRFPRVPGHRAGLLPGDFT
                     AVMEKRGFVLGVMGLNSAFLQLSDGDHTGKLDVGLQQFHAAAGGNASRWAEDCHAALL
                     LTHHPVTWLTPDARQTFSDEIAGHFLAHLYGHMHEPELAQQRLLGSTSGYRWLQGRSL
                     FGLEYWGPRRARSHGYSVGRLTVADDRATLQVWPRLLVNQKMVPDHAAADLHRTRHCA
                     TETVALRQPFIHRSPAAKRGDALPGPDAPFDRHWYVHRSGWEARALGYLDVLGKPATI
                     LGPKDIGKTWLCKYVCDLLRTRVSDPVRVAEVDVGGLVARTGANTTESFLRELCLRVG
                     AELALARAAVDERWRQTDGAPGERATAVFEGLLLPASPTPLVVAIDRLEAIPAAVRKD
                     LFGLLRSWCDCKAQPPWDQLRLLLVVPRIPNLGDLQSPFTITQAIPIEAFSIEEAEEL
                     VSYYGVRSTTRELASAHRKLGGHPLWLRKAAYEAKARPAGLAEVVDDLVAVIADDYRQ
                     RIDRKAPWRDALVRIVKGKPAGVKPATLDELYDAGFIVRKESAPLSYEPRMKEPLLAA
                     LES"
     gene            1888..>3408
                     /locus_tag="VF516_23900"
     CDS             1888..>3408
                     /locus_tag="VF516_23900"
                     /inference="COORDINATES: protein motif:HMM:NF025869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA-like domain-containing protein"
                     /protein_id="HEX8110805.1"
                     /translation="MARFQDEGGVVADGTFYVEREADRTLLERLREGRLCVVLAPRQI
                     GKSSLRDRTQRRLADAGITAVHIDVSAYTGADATEHWFYGGVIREIARRLGWDESRVL
                     ADGSDRPPAGWWNEFVQRELAERLDRPTVIFLDEIDSVRKLAFEPDALFTSIRLAMNQ
                     PPATFCLLGTMTPWEILRNPKAPPFNAGVQIRLDDFTADEARALLPGLAPLGDAEAIL
                     DEVLRWTSGHPYLTQRVCAALVAAGSRDVAAVVDTTLLQQHGDPLFAYTETAFDLYAG
                     GPAGVSLVELYGRLLRGERIAPDASSREQLELRVTGMAAERDAGGGRAIAVRNRIYAR
                     HFDDAWLKRRRIHRFLAAAVTHWRESKHTRGLLGRGDLDDVVAWASAQPSVSGEDLAF
                     LIASQEAALRQRRVVRGNLLIAGFAASLVLLIATVWLAVAHRHTASDLAAQAATIASL
                     TQRIDQLKTQQKNEIDSLKAQLAVAQQLADSRKQVNDAIQTSLDKFNATLARLEQEK"
BASE COUNT          459 a         1333 c         1172 g          444 t
ORIGIN      
        1 ccccgcgctc gaccgcaccc tgctcgcgat ctggaactgc ctcgagtcgc tgggctcgca
       61 cccggtgctg ctgaccgtgc ccggcaacca cgatctcgtc cggccgccgg ccggcgatcc
      121 ccgcctcgcc gagctcgcgc gctggcccgg cgatccggcg gtcgcgcagc ggttctgggc
      181 cgagccggcg tcgctccagc gcgcgctggt gaccgaggcg ttcgccccct acacgcggtg
      241 gtggagcgat caccgctttc cgcgcgtccc cggccaccgc gcgggcctct tgcccggcga
      301 cttcaccgcg gtgatggaga agcgcggctt cgtgctcggc gtgatggggc tcaacagcgc
      361 gttcctccag ctgtccgacg gcgatcacac cggcaagctc gacgtcggcc tccagcagtt
      421 ccacgccgcc gcgggcggca acgcgtcgcg ctgggccgag gactgccacg ccgcgctgct
      481 gctcacccac cacccggtga cctggctgac ccccgacgcc cggcagacct tcagcgacga
      541 gatcgccggc cacttcctgg cccacctcta cggccacatg cacgagcccg agctcgccca
      601 gcagcgcctg ctcggctcga cgagcggcta tcgctggctg cagggccgct cgctgttcgg
      661 gctcgagtac tgggggcccc ggcgcgcgcg cagccacggc tactcggtcg gccggctcac
      721 cgtcgccgac gaccgggcga ccctgcaggt ctggccgcgc ctgctggtca accagaagat
      781 ggtccccgac cacgccgccg ccgatctgca ccggaccagg cactgcgcga ccgagaccgt
      841 ggcgctgcgc cagccgttca tccaccgctc gccggcggcc aagcgcggcg atgcgctgcc
      901 cggccccgac gcgccgttcg accgccactg gtacgtccac cgctcgggct gggaggcccg
      961 ggcgctcggc tacctcgacg tcctcggcaa gccggcgacc atcctcggcc ccaaggacat
     1021 cggcaagacc tggctgtgca agtacgtctg cgatctgctg cgcacccggg tctcggatcc
     1081 ggtgcgcgtc gccgaggtcg acgtcggcgg cctggtcgcg cggaccggcg ccaacaccac
     1141 cgagagcttc ctgcgcgagc tctgcctgcg agtcggggcc gagctcgcgc tcgcgcgggc
     1201 cgcggtcgac gagcggtggc ggcagaccga cggcgccccc ggcgagcgcg cgaccgccgt
     1261 gttcgagggc ctgctgctgc cggcgtcgcc cacgccgctg gtcgtcgcga tcgatcgcct
     1321 cgaggcgatc ccggccgcgg tgcgcaagga cctgttcggc ctgctgcgca gctggtgcga
     1381 ctgcaaggcg cagccgccgt gggaccagct gcggctcttg ctcgtcgtgc cgcggatccc
     1441 caacctcggc gacctgcagt cgccgttcac gatcacccag gcgatcccga tcgaggcgtt
     1501 ctcgatcgag gaggccgagg agctggtctc gtactacggc gtgcgctcga ccacccgcga
     1561 gctggcctcc gcgcacagga agctcggcgg ccacccgctg tggctgcgca aggccgccta
     1621 cgaggccaag gcgcgcccgg ccggcctggc cgaggtcgtc gacgacctcg tcgcggtcat
     1681 cgccgacgac taccgccagc ggatcgaccg caaggcgccg tggcgcgacg cgctcgtgcg
     1741 gatcgtcaag ggcaagccgg cgggcgtcaa gccggccacg ctcgacgagc tctacgacgc
     1801 cggcttcatc gtccgcaagg agtccgcgcc gctgtcgtac gagccgcgga tgaaggagcc
     1861 gctgctcgcg gcgctggaga gctagccatg gcgcgcttcc aggacgaagg cggggtggtc
     1921 gccgacggca cgttctacgt cgagcgcgag gccgatcgca cgctgctgga gcggctgcgc
     1981 gagggccggc tgtgcgtcgt gctcgcgccg cgccagatcg gcaagtcgag cctgcgcgat
     2041 cgcacccagc gccggctggc cgacgccggg atcaccgccg tgcacatcga cgtcagcgcc
     2101 tacaccggcg ccgacgccac cgagcactgg ttctacggcg gcgtgatccg cgagatcgcg
     2161 cgccggctgg gctgggacga gagccgcgtg ctcgccgacg gctccgaccg gccgcccgcc
     2221 ggctggtgga acgagttcgt gcagcgcgag ctggccgagc ggctcgaccg gcccaccgtg
     2281 atcttcctcg acgagatcga cagcgtccgc aagctcgcgt tcgagcccga cgcgctgttc
     2341 acgtcgatcc ggctcgcgat gaaccagccg ccggcgacgt tctgcctgct cggcacgatg
     2401 acgccgtggg agatcctgcg caaccccaag gccccgccgt tcaacgccgg cgtgcagatc
     2461 cggctcgacg acttcaccgc cgacgaggcc cgcgcgctgc tgcccggcct ggcgccgctc
     2521 ggcgacgccg aggcgatcct cgacgaggtg ctgcgctgga cctcgggcca cccgtacctg
     2581 acccagcggg tgtgcgccgc gctggtcgcc gccggcagcc gcgacgtcgc cgccgtggtc
     2641 gatacgaccc tgctccagca gcacggcgat ccgctgttcg cctacaccga gaccgcgttc
     2701 gacctgtacg ccggcggccc ggccggcgtc tcgctggtcg agctctacgg ccggctgctg
     2761 cgcggcgagc ggatcgcccc cgacgccagc agccgcgagc agctcgagct ccgcgtcacc
     2821 ggcatggccg ccgagcgcga cgccggcggc ggccgcgcga tcgcggtgcg caaccggatc
     2881 tacgcccggc acttcgacga cgcctggctc aagcgccgcc gcatccaccg cttcctggcc
     2941 gccgcggtca cccactggcg cgagtccaag cacacgcgcg gcctgctcgg ccgcggcgac
     3001 ctcgacgacg tcgtcgcgtg ggcgagcgcg cagccgagcg tatccggcga ggacctcgcg
     3061 ttcctgatcg ccagccagga ggcggccctg cgccagcgcc gggtcgtgcg cggcaacctc
     3121 ctgatcgccg ggttcgccgc gagcctggtg ctcctgatcg cgacggtctg gctcgccgtg
     3181 gcccaccgcc acaccgcgag cgacctcgcc gcccaggccg ccacgatcgc gtcgctgacc
     3241 cagcgcatcg accagctcaa gacccagcag aagaacgaga tcgactcgct caaggcccag
     3301 ctcgcggtgg cgcagcagct cgccgacagt cgcaagcagg tcaacgacgc gatccagacc
     3361 agcctcgaca agttcaacgc caccctcgcg cggctggagc aggagaag
//
LOCUS       DASVOR010001253         4812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_451068,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001253 DASVOR010000000
VERSION     DASVOR010001253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4812
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451068"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /locus_tag="VF516_23905"
     CDS             <1..999
                     /locus_tag="VF516_23905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110806.1"
                     /translation="PGSAAEARAEARIDASPRDTKVSALRGGSRLTGEPGAELAMSRG
                     ETALLTRSGAIRVLEAIPGYFDFRVAAGESLTIHDPKPPTAVQFQFDGNCPDGGIIEL
                     DRDARFRTAKVSAGRDFANALIASGAWAYRLRCTRKDTEGGAVASGRITVVRDDGRRP
                     LPRAQGVNDIDADGRTYRISYQSAIPNVVVHVRNPGAAHRLHLASAGKEQIFDSQAAA
                     ITVPGGQLREGAYTYWIDRDGVKQDKVSTMIIDFDQTAPQVYIESPINGQPWTGDIDV
                     RGAVLPGWSAAVEEIAIPIDPQRRFTAKVGAPSGKALAIRLSHPQRGVHYYLRRPK"
     gene            1152..2171
                     /locus_tag="VF516_23910"
     CDS             1152..2171
                     /locus_tag="VF516_23910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110807.1"
                     /translation="MAGEDPSGGDTGGDPSGADGSDSGSGTGTTSGGMTTDPMVTATV
                     GPYFTTPMFFNTDVHALAKAASSDAIIAALVAEGGWGNSNKMQIDFDLDVLTASASTP
                     KQSFTPTSDFFSPDCDHVAMPVPAGGNLEGETGYACTHDGDCHLLVFDPSGGKLYEMW
                     RANITTAFQGGCLAVWPTATTYSSTLRGDQCTSADAAGFPISPLLFTADEVKAGHIDH
                     AIRFILPNNRVKRGYVRPATHGTSTTGTSAAPSYGVHLRLRADYPVSSLPAGAQVVAR
                     AMQKYGMYHADGGNIALTAQSDRHTTAKWAGLLGSTDLAALKVTDFEVIDHGAPIALT
                     GDCAR"
     gene            2227..3777
                     /locus_tag="VF516_23915"
     CDS             2227..3777
                     /locus_tag="VF516_23915"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8110808.1"
                     /translation="MRWVTRLTTAAVIAIAIAGAALAIRARMPSTHVGGEFRTYAMFR
                     DASRLAVGAAVVIAGVQVGVIDKLTVEGDLARIDLRLRDGLELPADSFATKRADSLFG
                     DSYIEIIPCCGEGSAIRLLRPGEPITHVVEGGSTDAALRAIAAAMPKIDSALDAVHDF
                     AIKGRSWVTGPVADRITGADTWVAEGHIERPIESADRIGSRIDDATVRVSDALATGGP
                     EALRTLDRIDGAITSARTRMHDARDGLVSALGDTRAGLDRIDPQLDQAREFVTAIHEG
                     RGTDWKGTLGRLVNEPELGNVIEDFTDDARDAVAGLNRFKSWIGGRVEVDAFSRDVRF
                     YASAEIRARHDKFYLVEIERGPLGGLPHDALSDVVNSPDYIRTVLIHDQPRFTFQFGK
                     QFGALALRGGIKDSTFGFGADALMLDGRLKLSADAYGSFTATPRLKLTGALAVFHSLY
                     VLAGVDDALNPPGYLQVLSGNPGAPQVLDKVRYGRDYFLGTSLQFTDEDVAVLLRIYG
                     AILVGALL"
     gene            complement(3820..4218)
                     /locus_tag="VF516_23920"
     CDS             complement(3820..4218)
                     /locus_tag="VF516_23920"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX8110809.1"
                     /translation="MSLVLIDTSVWARQNQPQVRQAVADAVEANAVAMVVPVLLELLR
                     SARNHAELQELADEYEALHVIPLDPELGRRAREVQAALARRGYHRGPSPVDLITAAAA
                     ESVDAELWHCDRHFELIAQVTGQRVRRLGR"
     gene            complement(4215..4451)
                     /locus_tag="VF516_23925"
     CDS             complement(4215..4451)
                     /locus_tag="VF516_23925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110810.1"
                     /translation="MRTTIDVDRRAAEEAAKVLQTESLKDTVNAALFEVLAAARRRRL
                     AGRIRAGALTAPTPAELARLRAPRVPAGALKGRR"
BASE COUNT          733 a         1770 c         1643 g          666 t
ORIGIN      
        1 cccggttcgg cggccgaggc ccgtgccgag gcccgaatcg atgcctcgcc gcgcgacacc
       61 aaggtcagcg cgctgcgcgg cgggtccagg ctgaccggcg agcccggcgc cgagctcgcg
      121 atgagccgcg gcgagaccgc gctgctgacc cggtccggcg cgatccgcgt cctcgaggcg
      181 atccccggct acttcgactt ccgggtcgcg gccggcgagt cgctgacgat ccacgatccg
      241 aagccgccga cggcggtgca gttccagttc gacggcaact gccccgacgg cgggatcatc
      301 gagctcgacc gcgacgcgcg gttccgcacc gccaaggtca gcgccggccg cgacttcgcc
      361 aacgcgctga tcgccagcgg cgcgtgggcc taccggctgc gctgcacccg caaggacacc
      421 gagggcggcg cggtcgcatc cggccggatc accgtggtgc gcgacgacgg ccggcggccg
      481 ctgcccaggg cccagggcgt caacgacatc gacgcggacg gccgcacgta ccggatctcg
      541 taccagagcg cgatccccaa cgtggtggtc catgtccgga acccgggcgc ggcgcaccgc
      601 ctgcatctcg cgtcggccgg caaggagcag atcttcgact cccaggccgc ggcgatcacc
      661 gtgcccggcg gccagctgcg cgagggcgcg tacacctact ggatcgatcg cgacggcgtg
      721 aagcaggaca aggtctcgac gatgatcatc gacttcgatc agaccgcgcc gcaggtctac
      781 atcgagtcac cgatcaacgg tcagccgtgg accggcgaca tcgacgtgcg cggcgcggtg
      841 ctgccgggct ggtcggcggc ggtcgaggag atcgcgatcc cgatcgaccc gcagcgccgg
      901 ttcacggcga aagtcggagc cccgagcggc aaggccctcg cgatccggct ctcccatccc
      961 cagcgcgggg ttcactatta cctgcgccgg ccaaagtaag cgaaccgggt gttctgaagc
     1021 ccagagaccc atcgagtatt ttttgcagta cgtgctcttt cgctcgtgca tctccgtcac
     1081 acgtgctctg ttgcctcatg cgagcaatcg tgacactggg attttttctg agcgcagcgt
     1141 gcggtgcgac gatggccggg gaggatccga gcggcggtga cacggggggg gatccgtcgg
     1201 gagcggacgg cagcgacagc ggcagcggca ccggcaccac cagcggcggc atgaccacgg
     1261 acccgatggt gaccgccacg gtcggcccgt acttcaccac gccgatgttc ttcaacaccg
     1321 acgtccacgc cctggccaag gcggcgagct cggacgcgat catcgcggcg ctggtcgccg
     1381 agggcggctg gggcaacagc aacaagatgc agatcgactt cgatctcgac gtgctgaccg
     1441 cgagcgcgtc gaccccgaag cagtcgttca ccccgacctc ggacttcttc tcgcccgact
     1501 gcgaccacgt ggcgatgccg gtgccggcgg gtggcaacct cgagggcgag accggctacg
     1561 cgtgcaccca cgacggcgac tgccacctgc tggtgttcga cccgtcgggc ggcaagctgt
     1621 acgagatgtg gcgcgcgaac atcacgaccg cgttccaggg cggctgcctc gcggtgtggc
     1681 ccaccgcgac cacgtactcg agcacgctgc gcggcgatca gtgcacgagc gccgacgccg
     1741 cggggttccc gatctcgccg ctgctgttca ccgccgacga ggtcaaggcc ggccacatcg
     1801 atcacgcgat ccgcttcatc ctgcccaaca accgggtcaa gcgcgggtac gtgcggccgg
     1861 cgacccacgg caccagcacg accgggacca gcgcggcgcc atcgtacggc gtccacctcc
     1921 ggctgcgcgc cgactatccg gtgtccagcc tgccggccgg cgcccaggtc gtcgcccgcg
     1981 ccatgcagaa gtacggcatg taccacgccg acggcggcaa catcgcgctg accgcgcaga
     2041 gcgatcgcca caccaccgcg aagtgggccg gcctgctcgg ctccaccgac ctcgccgcgc
     2101 tcaaggtcac cgacttcgag gtcatcgacc acggcgcgcc gatcgcgctg accggcgact
     2161 gcgcgcgcta gccgcgccct ccgctccagg gctgcgggcg gcggcgagcg ggggtagtat
     2221 tgccgggtgc gctgggtgac ccgactgacc accgcggcgg tgatcgcgat cgcgatcgcc
     2281 ggtgccgcgc tggcgatccg ggcccggatg ccgtcgaccc acgtcggcgg cgagttccgc
     2341 acctacgcga tgttccgcga cgcgtcccgg ctcgcggtcg gtgcggcggt cgtgatcgcc
     2401 ggcgtccagg tcggcgtgat cgacaagctc acggtcgaag gcgatctcgc gcggatcgac
     2461 ctgcggctgc gcgacggcct cgagctgccg gccgactcgt tcgcgaccaa gcgcgccgac
     2521 tcgctgttcg gcgacagcta catcgagatc atcccgtgct gcggcgaggg cagcgcgatc
     2581 cggctgctcc ggcccggcga gccgatcacc cacgtcgtcg agggcggctc gaccgacgcc
     2641 gcgctgcgcg cgatcgccgc cgcgatgccc aagatcgaca gcgcgctcga cgcggtccac
     2701 gacttcgcga tcaagggccg gtcgtgggtc accgggccgg tcgccgaccg gatcaccggc
     2761 gccgacacct gggtcgccga gggccacatc gagcgcccga tcgagtccgc cgaccggatc
     2821 gggtcgcgga tcgacgacgc caccgtgcgg gtcagcgacg cgctcgccac cggcggcccc
     2881 gaggcgctgc gcacgctcga ccggatcgac ggcgcgatca cgtcggcccg caccaggatg
     2941 cacgacgccc gcgacggcct ggtgtcggcg ctcggcgaca cccgggccgg gctcgaccgg
     3001 atcgacccgc agctcgacca ggcgcgggag ttcgtgaccg cgatccacga gggccgcggc
     3061 accgactgga agggcacgct cggccggctg gtcaacgagc ccgagctcgg caacgtgatc
     3121 gaggacttca ccgacgacgc gcgcgacgcg gtcgccgggc tcaaccgctt caagtcgtgg
     3181 atcggcgggc gggtcgaggt cgacgcgttc tcgcgcgacg tccggttcta cgccagcgcc
     3241 gagatccgcg cccgccacga caagttctac ctggtcgaga tcgagcgcgg cccgctcggc
     3301 ggcctgcccc acgacgcgct gtccgacgtg gtgaactcgc cggactacat ccggaccgtg
     3361 ctgatccacg accagccgcg gttcaccttc cagttcggca agcagttcgg cgcgctggcg
     3421 ctgcgcggcg gcatcaagga ctcgacgttc ggcttcgggg ccgacgccct gatgctcgac
     3481 ggccggctca agctgtcggc cgacgcctac ggctcgttca ccgcgacgcc gcggctcaag
     3541 ctgaccggcg cgctcgcggt gttccactcg ctctacgtcc tcgccggggt cgacgacgcg
     3601 ctcaacccgc cgggctacct gcaggtgctg tcgggcaacc cgggcgcgcc ccaggtgctc
     3661 gacaaggtcc gctacggccg cgactacttc ctcggcacct cgctgcagtt caccgacgag
     3721 gacgtcgcgg tgctgctccg gatctacggc gcgatcctgg tcggcgccct gctgtagctc
     3781 gtcggccgcc catgtgaacc gcgtcaccgc ctcgaacggt cagcggccga gtctgcgaac
     3841 ccgttgccct gtgacctgcg cgatcagctc gaagtgccgg tcgcaatgcc agagctcggc
     3901 gtcaaccgac tccgcggccg cagccgtgat gagatcgacc ggagacggtc cacgatggta
     3961 gccacgcctc gccagcgcag cctgtacctc acgagctcgc cggcccaact cgggatcgag
     4021 cggaatgacg tgaagcgctt cgtactcgtc ggcgagctcc tgcagctcgg catgattgcg
     4081 cgcggagcgc agcagctcca gcaggacagg gacgaccatc gcgaccgcgt tggcctccac
     4141 agcgtcggcg accgcctgcc ggacctgcgg ctggttctga cgcgcccaca cgctggtgtc
     4201 gatgaggacg aggctcaacg gcgacccttg agcgctccgg ccgggacacg gggagctcgc
     4261 aagcgcgcca gctcagccgg cgtcggcgcc gtcagggcac ctgcacggat ccggcctgcg
     4321 agccggcgcc gacgcgccgc cgccagcacc tcgaacagcg ccgcgttcac cgtgtccttc
     4381 agcgactcgg tctggaggac cttggctgct tcctcggctg ctctccggtc gacgtcgatg
     4441 gtggtccgca cggatatcca ggatggaccg caccagatat ctcgtcaagg catcacccga
     4501 ctttcaccac ccgtcctgcg cggaccccgc cccgacccca gcctcttcga gagaacgcgc
     4561 acacgcgagc tcgccgtggg ctgctgcgcg gatgggcgtc ggagccgcgc ccgccgaatc
     4621 accaccggcg cctgcgcgaa ccgcgttagg tatgtgcggg ggcaacgagt cccaatcgta
     4681 atcacgggtc gggtagcccg gcgacttggg ctcgccgggc ccccacagat ccggccaagc
     4741 ggaattcccg cagccggctc ttcggagaac aggttcgcta ggttccgtac cggtgaacga
     4801 tgcgcggggg tg
//
LOCUS       DASVOR010001254         4997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_509494,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001254 DASVOR010000000
VERSION     DASVOR010001254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509494"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="VF516_23930"
     CDS             complement(<1..241)
                     /locus_tag="VF516_23930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110811.1"
                     /translation="MTDRSLSSTVGIGVPGQGPDVDEALSTRLRDLALVEIDTLIQGD
                     RAQELVAAFADDVADALREARTRIAALRAQLGGADP"
     gene            complement(238..1035)
                     /locus_tag="VF516_23935"
     CDS             complement(238..1035)
                     /locus_tag="VF516_23935"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX8110812.1"
                     /translation="MGIFSRIKGGISGKANAAIDKAIDPAKELDMAILELEDGRKKAL
                     AELVSYKATAKQLDADMEKHRAKAAEWERRAMAAVKAGDDEAAKLALREKKAAEAEAV
                     KIERDKHEAASYAIQLNKSRKEFETKLQMLKMRKGTLATQLAAARSAGGDAFGNDSSV
                     WDRFAAAEERIDAEAIATEVDAAMRGEEIEAKAFDAKLAAAAGTANLLGAPGAPESED
                     ALAKLKAKMAEQKAAKQKQLAAEAGEAVNGAVNGAVNGATKEPKVDK"
     gene            complement(1087..1449)
                     /locus_tag="VF516_23940"
     CDS             complement(1087..1449)
                     /locus_tag="VF516_23940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110813.1"
                     /translation="MTTEEHHPQPHLHWMGTGHEIRARIEHALRVRSAPPGTAGAKLE
                     HVVKEAGARLWVQLKKRPSIGVVLVGGAGLVAASAVGVGEIAIGMIAGYAAYQVLREG
                     VSPGEAAKKIMEQIAKLG"
     gene            1882..2757
                     /locus_tag="VF516_23945"
     CDS             1882..2757
                     /locus_tag="VF516_23945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110814.1"
                     /translation="MLELVRERPRLVPDLLAKLDVSLPPFTEARLSDSALHEPAPIEH
                     FADAVVLLADEVRPVLGVIVEAQLRRDDQKLYSWPQYAMNARARHKCPFLVMVATPSL
                     AVAHWASQPIDVGNGTLFRPHVIGPDTIPKVTDAAQAAREPELAVLSTLIHASGNTPD
                     ASLIATAAIRGLSKLPEEQQLLYSTMIRNVLNAVLRKELETMVDIRKFMNATERRNYD
                     NAEAKGEAKGEAKGEARGKARGKAEAVLKILSKRNLPITPHHLRQINECTDLATLDRW
                     LDQALTVASVDELVD"
     gene            3005..3178
                     /locus_tag="VF516_23950"
     CDS             3005..3178
                     /locus_tag="VF516_23950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110815.1"
                     /translation="MARAEVKELLNFLTRRQLPITARQRQEICECADLTTLARWLGRM
                     GTVTSVDELLASP"
     gene            complement(3299..4729)
                     /locus_tag="VF516_23955"
     CDS             complement(3299..4729)
                     /locus_tag="VF516_23955"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8110816.1"
                     /translation="MTIGTIVCVDDQAEVRRLLGDVFRARGGSVVGFDDGEDAIAWLG
                     SNDADLAVLDLDLGPNRRSGIEICRALRARLPDLPIIILTGHGTIDDAVAAVKAGAVD
                     FITKDPYLEDKLEISVEKVERILQHALDRKRLEEENRELRATNERLRKAAGRRWQIVG
                     DSPAMRQVMTKIEKVAPVPRPVLVLGPRGTGKELVARAIHTLSPRASEPYITINCAAV
                     AESLLESELFGHEEGAFTGATKQKEGKFELADGGTLFLDEIGNMSLEFQAKILRVLEY
                     QRFERVAGSESIQVNVRVIAATNLDLKQAMLDGKFRPDLYDRLAFEVINLPPLAQRMQ
                     DVPVLAAHFLSRFRQEVAGIPVKEVSAGALDRFASYDYPGNVRELKNIVERAVYMAQG
                     EVLTAADVDAALPPEAAAQSAAPLLAFEDDVRLPLPERVEAFEAWLCKDALERTRYKQ
                     KEAAALLGLSYDQFRQRYRKYGLGKD"
     gene            complement(4726..>4997)
                     /locus_tag="VF516_23960"
     CDS             complement(4726..>4997)
                     /locus_tag="VF516_23960"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8110817.1"
                     /translation="PLVEIVISDTGPGIPRAHLSRLFVPGFTTKETGSGVGLAIAERV
                     VAAHHGRITVDSEEGRGTTITITLPTDKAGLQQLPLWTSAERGLP"
BASE COUNT          753 a         1802 c         1613 g          829 t
ORIGIN      
        1 gcggatcggc gccgccgagc tgggcgcgca gcgcggcgat ccgggtccgg gcctcgcgca
       61 gcgcgtcggc gacgtcgtcg gcgaacgccg cgaccagctc ctgcgcgcgg tcgccctgga
      121 tcagcgtgtc gatctcgacc agcgcgagat cgcgcagccg ggtcgacagc gcctcgtcga
      181 cgtcgggccc ctgcccgggc accccgatcc ccacggtgga cgacaggctg cggtccgtca
      241 cttgtccacc ttcggctcct tcgtcgcccc gttgaccgcc ccgttgaccg ccccgttgac
      301 cgcctcgccg gcctccgccg ccagctgctt ctgcttggcg gccttctgct cggccatctt
      361 cgccttgagc ttggccagcg cgtcctcgga ctccggggcg ccgggcgcgc cgagcaggtt
      421 cgccgtcccg gccgccgccg ccagcttggc atcgaatgcc ttggcctcga tctcctcgcc
      481 gcgcatcgcc gcgtcgacct cggtcgcgat cgcctcggcg tcgatccgct cctcggccgc
      541 cgcgaaccgg tcccacaccg acgagtcgtt gccgaacgcg tcgccgccgg ccgatcgcgc
      601 cgccgccagc tgcgtcgcca gcgtgccctt gcgcatcttc agcatctgca gcttggtctc
      661 gaactccttg cggctcttgt tgagctggat cgcgtagctc gccgcctcgt gcttgtcgcg
      721 ctcgatcttc accgcctccg cctcggcggc cttcttctcg cgcagcgcga gcttggccgc
      781 ctcgtcgtcg ccggccttga ccgcggccat cgcccggcgc tcccactcgg cggccttcgc
      841 acggtgcttc tccatgtccg cgtcgagctg cttggcggtg gccttgtacg acacgagctc
      901 ggcgagcgcc ttcttgcggc cgtcctcgag ctccaggatc gccatgtcga gctccttcgc
      961 cggatcgatc gccttgtcga tcgcggcgtt cgcctttcca gagatacccc ccttgatccg
     1021 cgagaagatg cccatggcgc gcagtctagc tcgaatcgcg gatcgcgatg gcccgctcgc
     1081 ccgcgatcac cctagcttcg cgatctgttc catgatcttc ttggccgcct ctcccggcga
     1141 gaccccttcc cgcagcacct ggtaggccgc gtagccggcg atcatcccga tcgcgatctc
     1201 gccgaccccc accgcactgg ccgccacgag cccggcaccg ccgaccagca cgacgccgat
     1261 cgacggccgc ttcttcagct ggacccagag ccgcgcgccc gcctctttca cgacgtgctc
     1321 cagcttcgcg cccgccgtcc ccggcggcgc gctgcggacg cgcagcgcat gctcgatcct
     1381 cgcacggatc tcgtggcccg tacccatcca gtgcaggtgt ggctgggggt gatgctcctc
     1441 ggtggtcatg gcggtcatga cgtatgcgcg ttgcaatgcc cgatccctcc gtccggcgtc
     1501 ccgatccacg ccggtccaca ggacggcgat cccacgatgc gcttgcgccc gcgacacagc
     1561 gtgcggcgcc gcgcacccat gtccttcgca cgaattccct gggcgcgaga ccagagcaat
     1621 gcgctgacct cgtgatgacc acccgcccct gcacggcagc ccagggcgag ccctcgatgg
     1681 cgcgctcacg gatgtgagct gccacaccag atgtgcgatc ggattaccga gcctgtagtc
     1741 gtgctcacca tttgggctct gcactgtcat ccggatctca gacattcgtc cggatttcgg
     1801 acatctagta tcgcaatcgt cagatccact tgatagggtg cattccgcat cgcgatgacg
     1861 gcctccagcc accacgagac cgtgctcgag ctcgtccgcg agcgcccccg gctcgtcccg
     1921 gatctgctcg ccaagctgga tgtctcactg ccacccttca ccgaagcgcg cctcagcgac
     1981 agtgcactcc acgagcccgc tcccatcgag cacttcgccg acgcagtcgt gctgctcgct
     2041 gacgaagtcc gcccggtgct cggggtcatc gtcgaagcac agctgcgccg cgacgaccag
     2101 aagctctaca gctggccgca gtacgcgatg aatgctcgcg ctcgccacaa gtgcccgttc
     2161 ctcgtgatgg tcgccacgcc gagcctggcc gttgcacact gggccagcca gccgatcgat
     2221 gtcggtaacg gcacgttgtt ccggccgcat gtcattggcc ccgataccat cccgaaggtc
     2281 accgatgccg cgcaggcagc acgagaaccg gagctcgcgg tactgtcgac gctcatccac
     2341 gcaagcggca acacgccgga cgcaagcctc atcgccaccg ccgcaatccg ggggctatcc
     2401 aagctccccg aggagcagca gctgctatac tcgacgatga ttcggaacgt gctgaacgct
     2461 gtcctccgaa aggaactcga aacgatggtc gacatccgca aattcatgaa cgccacggaa
     2521 cggcgcaact acgacaacgc ggaggccaaa ggggaggcca aaggggaggc caaaggcgag
     2581 gccagaggca aggccagagg caaggccgag gccgtgctga agatcctgag caagcgtaat
     2641 ctaccgatca cgcctcacca cctgcgccag atcaacgagt gcaccgacct cgccacactg
     2701 gaccgctggc tcgatcaggc gctcacagtg gcatccgtcg acgagctcgt tgattaggag
     2761 caccgatgcg gaagcaaacc acaacaccgg aacacggatc gctcagcggg gcaagcgact
     2821 gttccctggc cgagcgcacc agcgacgtgc aagacgacca aactcctttc gaaccaatga
     2881 gtcagttcat catcaagata gacatcgaga cctgtgcgat gctatcgagc ggaaccggca
     2941 tcgcctcggc ccggagtttc tggaagagta cgaccgcgtc ttcgccaaga acatagtcaa
     3001 ggccatggcc agggcggagg tcaaggagct gctgaacttc ctgactcgtc gacaattgcc
     3061 catcaccgct cgccagcggc aagagatttg cgaatgcgcc gatctgacga cactcgctcg
     3121 ctggctgggt cggatgggga ccgtcacgtc ggtcgatgag ctcctcgcgt cgccgtgacc
     3181 gctcctcatg ttgtcgtggg tgccgcgcga cagggccctc gcaccaccgg gcctcaacac
     3241 ctcgagccgc cccgacatca gtagtccgcg tcgacgtccg cgggcggacg gttcggcctc
     3301 aatccttgcc caagccgtac ttgcggtagc gctggcggaa ctggtcgtag gacaggccga
     3361 gcagggcggc ggcctccttc tgcttgtagc gggtgcgctc gagggcgtcc ttgcacagcc
     3421 aggcctcgaa ggcctcgacc cgctcgggca gcggcagccg gacgtcgtcc tcgaatgcga
     3481 gcagcggggc ggcgctctgg gcggcggcct cgggtggcag cgcggcgtcg acgtcggccg
     3541 cggtcagcac ctcgccctgg gccatgtaga ccgcgcgctc gacgatgttc ttgagctcgc
     3601 ggacgttgcc cgggtagtcg tacgacgcga agcggtcgag cgcgccggcg ctgacctcct
     3661 tgaccgggat gccggcgacc tcctggcgga accggctgag gaagtgggcg gcgagcaccg
     3721 gcacatcctg catgcgctgg gcgagcggcg gcaggttgat cacctcgaac gcgagccggt
     3781 cgtagagatc ggggcggaac ttgccgtcga gcatcgcctg cttgaggtcg aggttggtcg
     3841 ccgcgatcac gcggacgttg acctggatcg actcgctgcc ggcgacccgc tcgaaccgct
     3901 ggtactcgag gacgcgcagg atcttggcct ggaactcgag cgacatgttg ccgatctcgt
     3961 cgaggaacag cgtaccgccg tcggcgagct cgaacttgcc ttccttctgc ttggtcgcgc
     4021 cggtgaacgc gccctcctcg tggccgaaca gctcgctctc gagcaggctc tcggcgaccg
     4081 cggcgcagtt gatcgtgatg tagggctcgg acgcccgcgg cgacagggtg tggatcgcgc
     4141 gcgcgaccag ctccttgccg gtgccgcgcg gaccgagcac gagcaccggc cgcggcaccg
     4201 gggcgacctt ctcgatcttg gtcatcacct ggcgcatcgc cggcgagtcg ccgacgatct
     4261 gccagcgccg gccggcggcc ttgcgcagcc gctcgttggt cgcgcgcagc tcgcggttct
     4321 cctcctcgag gcgcttgcgg tcgagcgcgt gctggaggat gcgctcgacc ttctcgacgg
     4381 agatctccag cttgtcctcg aggtacggat ccttggtgat gaagtcgacg gcgccggcct
     4441 tgaccgccgc gaccgcgtcg tcgatcgtgc cgtggccggt caggatgatg atcggcaggt
     4501 cgggcagccg ggcgcgcagc gcgcggcaga tctcgatgcc gctgcgccgg ttggggccga
     4561 ggtcgaggtc gagcaccgcg agatcggcgt cgttgctgcc gagccaggcg atcgcgtcct
     4621 cgccgtcgtc gaacccgacc acgctgccgc cgcgggcgcg gaacacgtcg ccgagcaggc
     4681 ggcggacctc ggcctggtcg tcgacgcaga cgatcgtgcc gatcgtcacg gcagccctcg
     4741 ctcggcggag gtccacagcg gcagctgctg gaggccggcc ttgtcggtcg gcagcgtgat
     4801 cgtgatcgtc gtgccgcggc cctcctcgga gtcgaccgtg atccggccgt ggtgcgccgc
     4861 gaccacgcgc tcggcgatcg ccaggccgac gcccgagccg gtctccttgg tggtgaagcc
     4921 ggggacgaac agccggctga ggtgggcgcg cgggatgccc ggcccggtgt cgctgatcac
     4981 gatctcgacc agcggcg
//
LOCUS       DASVOR010001255         3609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_392297,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001255 DASVOR010000000
VERSION     DASVOR010001255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3609
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392297"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..948)
                     /locus_tag="VF516_23965"
     CDS             complement(148..948)
                     /locus_tag="VF516_23965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110818.1"
                     /translation="MRRRPVLQRPPGNYGSRIFALLQPGTYHLIVDGFDASQFGSYTL
                     RVKFAPGCVPNCDGQYCGGDDGCGGTCGDCASGEACGVDFRCRPAPCVPDCKNADNSP
                     RTCGDDGCLGSCGMCGADEMCVPATGTCETFAACDHVHPTCPGGCPARNFCGTDCACH
                     SIDDPLPDLVINEKRLADEILFDHINVRDSSCSFVEQCVGGLGDRRVMRFSVEAINQG
                     QATLTVPPPDQRPDLLVFSTCRDGACVRRVRGRRGAARQRATAFAWML"
     gene            1418..3025
                     /locus_tag="VF516_23970"
     CDS             1418..3025
                     /locus_tag="VF516_23970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8110819.1"
                     /translation="MARTPLLGSLRRLVRDARISRQTGIPVDELPDLRAARRLSRRDL
                     LTGVAAGAAVLALPSRVRAAGQPKVVIVGGGIAGLTCALTLRDEGFCSTVYEASGRIG
                     GRMFSNNRGYWAHNQVSEWCGELIDTGHQTIQDLAARYSLALDDVRAAEPHRSSEVYK
                     FGGQYYPAAQATADFLAMFDALQADVDAAPFPTLFNSFTAAGLALDHMSVYDWIESRV
                     PGGHRAPLGQLLDVAYTAEYGAESRVQSSLNLVYLLGFQSTATRLDLYGESDERFHIR
                     GGNQLLPQAIADDLGDDVVRTGHRLVRLARTAGGRYRCTFERAGGSHEVTADFVVLAI
                     PFAVLADVDTSRAGFDELKRIAIAELGRGHNGKLQLQFARRGWLGTGPWPGTSNGMSF
                     ADTGYQQTVEVTRAQPGTPGILDLYSGGHVTDRMRTTSPFATARDLGVLEDVARGLGE
                     LAAVYPGLIWNGKATQSLPHKSPFFHNSYSYWKVGQYTRFAGYEGVPLGGVHFCGEHT
                     STDFQGFMEGGAETGEQTAKDLAKLLR"
     gene            complement(3060..>3609)
                     /locus_tag="VF516_23975"
     CDS             complement(3060..>3609)
                     /locus_tag="VF516_23975"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX8110820.1"
                     /translation="PSPRSIPEVARPASIPTTGPTPGTTIDTLREIEQFLYRQSELLD
                     AKLWQDYIDLFTDDGVYWMPVTPEQTEWEGSPSIFAEDKHMMEVRMGRVTHPTAWSQA
                     PMWVTSHVLGNVVIEAEAPGEWRVRSRFHMMELRRDTVRHFGGTYRHTLVRQAGQLKI
                     KLQRVDMFNAQAPYDYVLQVWV"
BASE COUNT          545 a         1292 c         1241 g          531 t
ORIGIN      
        1 cgacggcgca cgccgtgccg cgctgaacgg gggcgcatgg tctgatcacc cggtcagaga
       61 ctcgcgcgcc gcatgcgccg ccatcgtcga ccgagccacg gcacgacggc acagatcccc
      121 accagcgcgc tctgcgcgtc gccggtatca cagcatccac gcgaaggcgg tggcgcgttg
      181 ccgggcggcc ccgcgtcggc cgcggacgcg ccgcacacag gccccgtcgc ggcacgtcga
      241 gaacaccaac aggtcgggtc gctgatccgg cggcggcacc gtcagcgtcg cctgcccctg
      301 gttgatcgcc tcgaccgaga accgcatgac ccggcgatcg ccgagcccgc cgacgcactg
      361 ctcgacgaag ctgcatgagc tgtcccggac gttgatgtga tcgaacagga tctcgtcggc
      421 caggcgcttc tcgttgatca cgaggtcggg cagcgggtcg tcgatcgagt ggcaagcgca
      481 gtcggtgccg cagaagtttc gcgccgggca gccccccgga cacgtcgggt gcacgtggtc
      541 gcacgcggcg aacgtctcgc aggtccccgt cgcggggacg cacatctcgt ccgcgccgca
      601 catgccgcag ctccccaggc agccgtcgtc gccgcacgtc cgcggcgagt tgtcggcgtt
      661 cttgcagtcc gggacgcagg gggcgggccg gcaccggaag tccacgccgc acgcctcgcc
      721 ggatgcacag tcaccgcagg ttccgccgca cccgtcgtcg ccgccgcagt actgcccgtc
      781 gcagttggga acgcagccgg gcgcgaactt cacgcgcagc gtgtacgagc cgaactgcga
      841 ggcgtcgaag ccatcgacga tgaggtggta cgtgccgggc tggagcagcg cgaagatgcg
      901 agatccgtag ttgccgggcg gccgttgcag aacaggccgt cgtcgcacag acgctccagg
      961 ttatcgccac agccggcgac gaggatcgcg gccaggaccc atcgggatcg atgcgtgggg
     1021 ctcacgctcg accgctgatc cgcatactcg gacatgttca ccctccatga cagctccgcg
     1081 acacgatgcg cgtgaatgcc tatgcactca cagcgctcgt cgtgcatggc cgcgcgaccg
     1141 cgcagtttga accgtggact gatcaatacc tgatatcaat acttccaatc gcggcagcgc
     1201 ctccgcggca aattgcgcaa cctctgagct gcagcgggtg atcggtgact cacgctgact
     1261 ggcaactgtg attgggcgcc gacgcacatc gcgatgtgtg cctggtcaac ctccccgtgc
     1321 gcccggggca tggcacccag gcatccgatg gcagatttca cgagcgcggg atcgcgatcc
     1381 tttgaggccg atcgggcgaa cgctacggtg gatcgccatg gcccgaactc ctctgctagg
     1441 tagcctgcgc cggctcgtgc gcgacgcgcg gatctcccgg caaaccggca tcccggtgga
     1501 cgagctcccc gacctgcgcg ccgcacgccg gctgtcgcgc cgcgacctgc tcaccggggt
     1561 ggccgccggg gccgcggtgc tcgcgctgcc gagtcgggtg cgcgccgccg gccagccgaa
     1621 ggtcgtcatc gtcggcggtg gcatcgcggg cctgacctgt gcgctcacgc tgcgcgacga
     1681 ggggttctgc tcgacggtgt acgaggcctc gggccggatc ggcggccgga tgttctcgaa
     1741 caaccgcggc tactgggcgc acaaccaggt cagcgagtgg tgcggcgagc tgatcgacac
     1801 cgggcaccag acgatccagg acctcgccgc gcgctacagc ctggcgctcg acgacgtccg
     1861 cgcggcggag ccgcaccggt ccagcgaggt ctacaagttc ggcggccagt actatcccgc
     1921 agcccaggcc acggccgact tcctggcgat gttcgacgcg ctgcaggccg acgtcgacgc
     1981 ggcgccgttt ccgaccctgt tcaactcgtt caccgccgcc ggcctcgcgc tcgatcacat
     2041 gagcgtgtac gactggatcg agtcgcgcgt gccgggcggc caccgcgcgc cgctcggcca
     2101 gctgctcgac gtcgcctaca cggccgagta cggcgccgaa tcgcgcgtcc agtcgtcgct
     2161 caacctggtc tacctgctcg gcttccagtc gaccgcgacc cggctcgacc tctacggcga
     2221 gtccgacgag cgcttccaca tccgcggcgg caaccagctg ctgccgcagg cgatcgccga
     2281 cgacctcggc gacgacgtcg tccggaccgg ccaccgcctg gtgcggctgg cccggaccgc
     2341 cggcggccgc taccgctgca cgttcgagcg cgccggcggc tcgcacgagg tcaccgcgga
     2401 cttcgtcgtg ctggcgatcc cgttcgcggt gctcgccgac gtcgacacct cgcgcgccgg
     2461 cttcgacgag ctcaagcgga tcgcgatcgc cgagctcggc cgcggccaca acggcaagct
     2521 ccagctccag ttcgcgcggc gcggctggct cggcaccggg ccgtggccgg gcacgtcgaa
     2581 cggcatgagc tttgccgaca ccggctacca gcagaccgtg gaggtcacgc gcgcccagcc
     2641 gggcacgccg ggcatcctcg acctgtactc cggcggccac gtgaccgacc ggatgcgcac
     2701 cacgtcgccg ttcgcgaccg cgcgcgatct gggggtgctg gaggacgtgg cgcgcgggct
     2761 cggcgagctc gccgcggtct acccggggct aatctggaac ggcaaggcga cgcagtcact
     2821 tccccacaag tcgccgttct tccacaactc gtactcgtac tggaaggtcg ggcagtacac
     2881 caggttcgcg ggctacgagg gcgtcccgct cggcggcgtc cacttttgcg gcgagcacac
     2941 ctcgacggac ttccagggct tcatggaagg cggcgccgag accggcgagc agaccgccaa
     3001 ggacctcgcc aagctcctca ggtagcgatc cgcatcgccc tccgggcggc gcgatgccat
     3061 cacacccaga cctgcagcac gtagtcgtag ggcgcctggg cgttgaacat gtcgacccgc
     3121 tgcagcttga tcttgagctg gccggcctgg cgcaccaggg tgtggcggta ggtgccgccg
     3181 aagtggcgca cggtgtcgcg gcgcagctcc atcatgtgga accgcgagcg cacgcgccac
     3241 tcgccggggg cctcggcctc gatcacgacg ttgccgagga cgtggctggt cacccacatc
     3301 ggcgcctgcg accacgcggt gggatgggtg acgcggccca tccgcacctc catcatgtgc
     3361 ttgtcctcgg cgaagatcga cggcgagccc tcccactcgg tctgctcggg ggtgaccggc
     3421 atccagtaca cgccgtcgtc ggtgaacagg tcgatgtagt cctgccagag cttggcgtcg
     3481 agcagctcgg actggcggta caggaactgc tcgatctcgc gcagggtgtc gatggtggtc
     3541 ccgggggtcg ggccggtggt cgggatcgag gccggccggg cgacctcggg gatggagcgc
     3601 ggcgacggc
//
LOCUS       DASVOR010001256         7177 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421297,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001256 DASVOR010000000
VERSION     DASVOR010001256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7177
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421297"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF516_23980"
     CDS             complement(<1..189)
                     /locus_tag="VF516_23980"
                     /inference="COORDINATES: protein motif:HMM:NF014447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110821.1"
                     /translation="MRIRQHVNPNQVHFARFRGERPVLDGRPIELEVGCADAQFLFER
                     AAQDPARHYVGLEIREDLV"
     gene            complement(199..639)
                     /locus_tag="VF516_23985"
     CDS             complement(199..639)
                     /locus_tag="VF516_23985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEX8110822.1"
                     /translation="MTGDPGRRAFSVAVYARRGDRVLVIEHRRLATWLPIGGELEPGE
                     KPLEAARRELREETGLEGRFRPLVGALDGVPPGLLGYEEHPAGSKGLHMNFVFVAEVA
                     DGAEVAPNHEFTAWRWVDRGELDGVASPLNVRQFGVLALAARFG"
     gene            complement(636..1280)
                     /gene="fsa"
                     /locus_tag="VF516_23990"
     CDS             complement(636..1280)
                     /gene="fsa"
                     /locus_tag="VF516_23990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEX8110823.1"
                     /translation="MKFFIDTADVREIREAAAMGLVDGVTTNPSLVAKTGRKFKEVLL
                     EICDLVKGPVSAEVVGTTYDEMMREAREYAALRSNIVVKIPMLPEGMKAVRTCTTEGI
                     KTNVTLCFSATQALVAAKAGATYISPFVGRLDDVSTNGMQLIGEIVQIYDNYAFETEV
                     LVASVRHPMHVHEAAMLGAHVATCPLSVLLQLCKHPLTDLGLAKFLADWEKVPK"
     gene            1366..2115
                     /locus_tag="VF516_23995"
     CDS             1366..2115
                     /locus_tag="VF516_23995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110824.1"
                     /translation="MPPVRIGTVDIPERVERSRYFEELSYLELSALFAAPLKSASLAR
                     WQELAPPGALGLVAPWVLTHRKPPKSERLWHHDASVGDFRDSPLGRSALAAFSGAVTT
                     LAASLAIFPSPPLFAPSTANRDQLRRFFGEVATREAVGADRVWIPDGLWEPRAAVGFA
                     GELGVTCAIDPLVRDPARPAEVYEDLDASALYFRIAGLGRAGPLRAEALEDLAALLEH
                     YENIELTVAFASPTRWQDARNFKKLLEGAGS"
     gene            complement(2133..2276)
                     /locus_tag="VF516_24000"
     CDS             complement(2133..2276)
                     /locus_tag="VF516_24000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110825.1"
                     /translation="MAVAATRRSEPIIQMVERARTPAPPRVVAELPKKAGELPIPVAT
                     FVF"
     gene            2491..4023
                     /locus_tag="VF516_24005"
     CDS             2491..4023
                     /locus_tag="VF516_24005"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEX8110826.1"
                     /translation="MDANGGIELTLLQGGPQEAHVDLFAFTTFGDPSKDSFFKSVDSA
                     LSGVLSDVAKSESFEGKTGQSIVVHTHGRIPAKRILVVGAGGRSEFANHHMRDIAATI
                     AQVANKASAASVGFVLPAVAGGREQALVQILAEGVGLGTYKFGRYLTGEDHKRPMALK
                     RFGVFLDTKGRKPTAAQSKAFAAAVTRGTQIAQAVNHARDLINEPAAAITPTALASDA
                     QTIAKKHKSLAVTVLDAKKCQELGMGMFLAVGRGSDQEPRFIHMTYKPAKKPKKRLCF
                     IGKGITFDSGGYSLKTSQAMEDMKVDMSGAAAVISAMDAIATLGSEHEIHVIAACCEN
                     LVSGRAYKLGDVLTSMDGTTVEINNTDAEGRLTLGDAITYARTKIQPDEMFDFATLTG
                     ACMVALGPYTAGVMSDHDSLVKSWMSCAERTGEDMWRLPLTARLRDQLKSQIADMRNT
                     GDRFGGAITAGLFLKTFAKDTPWVHVDIAGPASLSTTRPSQPKGGTGFAVTTIVEYAT
                     RA"
     gene            4166..4666
                     /locus_tag="VF516_24010"
     CDS             4166..4666
                     /locus_tag="VF516_24010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEX8110827.1"
                     /translation="MKPQFAVGDKAVYPVHGVAEVMALEQRDIGGNKTSVYILKVLDT
                     GLKIMVPTNNAGSVGLRDLITSKQVKEVYSILKSRDIPRDTQTWNRRYREYMEKIKTG
                     SVFEIAEVLRDLCVLRLTKELSFGERKMLDTARGLLIKELAIAKGVGEDKISGEIDAI
                     FAPAAA"
     gene            complement(4812..5816)
                     /locus_tag="VF516_24015"
     CDS             complement(4812..5816)
                     /locus_tag="VF516_24015"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX8110828.1"
                     /translation="MAMLHRSRLLGIGMSVPPRVVTNHDLARMMETSNEWIIERTGIE
                     ERRWVSEGETGATLATAASKEAIERAGLSPRDIDMIIYATLSPDFNFPGTGVFVQRAL
                     GLRDIPCIDIRQQCTGFIYGMSIADAYIRTGAFKHILLIGAEVHSTGLDTSTAGRDVT
                     VLFGDGAGAVVVGRATDESHMILSTHIHADGSEAEILWTEYPASAHHPRISAEAMAER
                     KHYPVMNGKRVFKHAVTRMPATIMEGMVTNNLKLTDIDMMIPHQANLRINQMVGQMIG
                     LPPEKMHNNIQKYGNTTAASIPICMHEAIALGKIQPGHMVCLVAFGAGLTWGSVFLRY
                     "
     gene            complement(5981..>7177)
                     /locus_tag="VF516_24020"
     CDS             complement(5981..>7177)
                     /locus_tag="VF516_24020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX8110829.1"
                     /translation="KLADCQERDPEKSEIFIVEGESAGGSAKQGRSRQFQAILPLRGK
                     ILNVEKVRFDRMLSSQELTTLITALGTGVGDAKDLDKLRYHKIIIMTDADVDGSHIRT
                     LLLTFFFRHFNELFEKGHVYIAQPPLYKVKKGKTELYLKNEIALEDYLLDSVCRETVL
                     HRGSDSSPLAGHELVALVKRISQARRLREQLDKRGDGRITAQFAEAGLSEDDLKDRDK
                     LERLEAKVMAEVSRRHGELGQAAATYKQDIEHGTWEIRFPAGAHGIRRATVINSDVVR
                     SAEFVELRRASAELKATLTEPLSIQHEEGEARAIESWDEIATVIEELGRKGLQIQRYK
                     GLGEMNAEQLWETTMDPAKRNLLKVKVEEMEAADGIFTKLMGDLVEPRREFIEENALN
                     VRNLDV"
BASE COUNT         1145 a         2445 c         2355 g         1232 t
ORIGIN      
        1 gaccaggtcc tcgcggatct cgagcccgac gtagtggcgc gccggatcct gcgcggcgcg
       61 ctcgaacagg aactgcgcgt cggcgcagcc gacctcgagc tcgatcgggc ggccgtcgag
      121 caccgggcgc tcgccgcgga accgggcgaa gtggacctgg ttggggttga cgtgctgccg
      181 gatccgcacg ccgatccact acccgaagcg ggccgcgagc gcgagcacgc cgaactggcg
      241 gacgttgagc ggcgacgcca caccgtcgag ctcgccgcgg tcgacccagc gccaggccgt
      301 gaactcgtgg ttcggcgcga cctcggcgcc gtctgccacc tcggcgacga acacgaagtt
      361 catgtgcagg cccttggacc ccgccgggtg ctcctcgtag ccgagcaagc ccggcggcac
      421 cccgtcgagc gccccgacca gcgggcggaa ccgaccttcc agcccggtct cctcgcgcag
      481 ctcgcgcctc gcggcctcga gcggcttctc gcccggctcg agctcgccgc cgatcggcag
      541 ccaggtcgcg agccggcggt gctcgatcac cagcacgcgg tcgccccggc gcgcgtacac
      601 cgccaccgag aacgcccgcc ggcccgggtc gccggtcact tcgggacctt ctcccagtcg
      661 gcgaggaact tggccagccc gaggtcggtc agcgggtgct tgcacagctg gagcagcacc
      721 gagagcggac aggtcgcgac gtgggcgccc agcatcgccg cctcgtggac gtgcatcggg
      781 tggcggaccg acgcgaccag cacctcggtc tcgaacgcgt agttgtcgta gatctgcacg
      841 atctcgccga tcaactgcat cccgttggtc gagacgtcat cgagccggcc gacgaacggc
      901 gagatgtacg tcgctcccgc cttggcggcg accagcgcct gggtcgccga gaagcacagc
      961 gtgacgttgg tcttgatgcc ctcggtggtg cacgtgcgca cagccttcat gccctccggg
     1021 agcatcggga tcttgaccac gatgttgctg cgcagcgcgg catactcgcg cgcctcgcgc
     1081 atcatctcgt catacgtggt ccccacgacc tcggccgaca ccggcccctt cacgaggtcg
     1141 cagatctcga gcaggacttc cttgaacttg cgcccggtct tggcgaccag cgacggattg
     1201 gtggtcacgc cgtcgaccag ccccatcgcg gcggcctcgc ggatctctct gacgtcagca
     1261 gtgtcgatga agaacttcat ggcggacctc cattgcacga tagccgacga gcacgtagcg
     1321 tgccggcgcc ggcactttac gagccgggtc tgacttgttt ccaacatgcc ccctgtccgc
     1381 atcggcaccg tcgatatccc cgagcgcgtc gagcgctccc ggtactttga ggagctgtcg
     1441 tacctcgagc tgtccgccct gttcgccgcg ccgctcaagt ccgcgtcgct cgcccggtgg
     1501 caagagctgg caccgcccgg cgcgctgggc ctcgtggcgc cctgggtcct gacccaccgc
     1561 aagccgccca agtccgagcg gctgtggcac cacgatgcct cggtcggtga ctttcgcgac
     1621 agtccgctcg gccgctcggc gctggcggcg ttctcgggag cggtgaccac gctggcggcg
     1681 tcgctcgcga tcttcccgtc tcccccgctg tttgcgccgt cgaccgcgaa ccgcgaccag
     1741 ctccgccggt tcttcggcga ggtcgcgacc cgcgaggcgg tcggggccga ccgggtgtgg
     1801 atccccgacg ggctgtggga gccccgcgcc gcggtcgggt tcgccggcga gctcggcgtg
     1861 acctgcgcga tcgacccgct ggtccgggac cccgcccggc cggccgaggt ctacgaggac
     1921 ctggacgcct ctgcgctata tttccggatc gccgggctgg gccgggctgg gccgctccgg
     1981 gccgaagcgc tggaggattt agcagcgtta ctggagcatt acgagaatat cgaactgacc
     2041 gtcgcttttg cgtcaccaac ccggtggcaa gatgcccgca acttcaagaa actgctcgaa
     2101 ggagcagggt cttgagagaa gcactcaggc gatcagaaca cgaacgtagc gacggggatc
     2161 ggcagctcac cggccttctt gggcagctcg gcgacgacgc gagggggcgc cggagtccgc
     2221 gcacgctcca ccatctggat gatcggctcg gaccgtcgcg tcgccgcgac ggccacggtc
     2281 ttctttgtgc gacttccgtt gcccttccgc gccgctgggc gcgcagtcat caccttggat
     2341 cgcattcaaa ctccgggggg aatctgtttg tttgggtttc gactctacgg atcctgtgaa
     2401 tctactgtca atgtaggcgc tatgattcct ttacatcgga ctgcgagcgc cgtataacag
     2461 cgcccgcttc gctaggagtt ccaaatattt atggatgcca acggcggtat cgagctcacg
     2521 ttgctgcaag gtggcccaca agaggcccac gttgacctat ttgcgtttac tacattcggc
     2581 gatccgagca aggactcctt tttcaagtcg gtcgactccg cgctgagcgg cgtgctatcc
     2641 gacgtggcga agtcggaatc cttcgagggc aagaccggac agtcgatcgt ggtccacacg
     2701 cacggtcgca tcccggcgaa gcggattctc gtagtcggcg ccggtggaag aagcgagttt
     2761 gcaaaccatc acatgcggga catcgcggcc accatcgcgc aggtcgccaa caaggcgagc
     2821 gcagcgtcgg tcggcttcgt gctcccggcg gtcgccgggg gccgcgagca ggcgctggtc
     2881 cagatcctcg ccgagggggt cggcctcggg acgtacaaat tcggacggta tctcaccggc
     2941 gaggaccaca agcgcccgat ggcgctgaag cggttcgggg tgttcctcga caccaagggc
     3001 agaaagccga ccgccgcgca gtccaaggcg ttcgccgcgg ccgtgacgcg cggcacacag
     3061 atcgcccagg cggtcaatca cgcgcgcgac ctgatcaacg agccggcggc ggcgatcacc
     3121 cccaccgcgc tggccagcga cgcccagaca atcgccaaga agcacaagtc gctggccgtc
     3181 accgtgctcg atgccaagaa gtgtcaggag ctcggcatgg ggatgttcct cgcggtcggt
     3241 cggggctccg accaggagcc ccgcttcatc cacatgacct acaagccggc caagaagccc
     3301 aagaagcggc tgtgcttcat cggcaagggc atcacgttcg actccggcgg ctactcgctc
     3361 aagacgtcgc aggcgatgga ggacatgaag gtcgacatgt ccggcgccgc cgccgtgatc
     3421 tcggcgatgg acgcgatcgc cacgctcggc tccgagcacg agatccatgt cattgcggca
     3481 tgctgcgaga acctcgtgtc cggccgcgcc tacaagctcg gcgacgtgct gacctcgatg
     3541 gacggcacca ccgtcgagat caacaacacc gacgccgagg gccggctgac cctgggcgac
     3601 gcgatcacgt acgcgcgcac caagatccag cccgacgaga tgttcgactt cgccacgctg
     3661 accggcgcct gcatggtcgc gctcgggccg tacaccgccg gcgtgatgtc cgaccacgac
     3721 agcctggtca agtcctggat gtcgtgcgcc gagcgcaccg gagaggacat gtggcggctg
     3781 ccgctcaccg cgcggctgcg cgatcagctc aagagccaga tcgccgacat gcggaacacc
     3841 ggcgaccggt tcggaggcgc gatcaccgcc ggcctgttcc tcaagacgtt cgccaaggac
     3901 acgccgtggg tccacgtcga catcgcgggc ccggcatcgc tgtccaccac gcgtccgtcg
     3961 cagcccaagg gcggaaccgg cttcgccgtg acgaccatcg tggaatatgc cacgcgagcc
     4021 tgaaacctac atggtttcct ccgaaaaagt cttcccgggg tgatcctcgt tacgtggctt
     4081 gcacggggta tcccctagtt aactatgcga gtcctttgac atacgcgttg ttccctgcta
     4141 ccttacattc gttggagcca ctgacttgaa accccaattt gcagtcggcg acaaggccgt
     4201 ctatccggtc cacggggtag ccgaggtgat ggcgctcgag cagcgtgaca tcggtgggaa
     4261 caagacctcc gtgtacatcc tcaaggtgct cgacaccggg ctgaagatca tggtgccgac
     4321 gaacaacgcc ggctccgtgg gcctccgcga cctcattacc tcgaagcagg tcaaggaggt
     4381 ctactcgatc ctcaaatcgc gcgatatccc ccgcgatacc cagacctgga accggcgcta
     4441 tcgcgagtac atggaaaaga tcaagaccgg cagcgtgttc gagatcgccg aggtcctgcg
     4501 cgacctctgc gtgctgcggc tgaccaagga gctgtcgttc ggcgagcgca agatgctcga
     4561 taccgcgcgc ggcctgctga tcaaggagct cgccatcgcc aagggcgtgg gcgaggacaa
     4621 gatcagcggc gagatcgacg cgatctttgc ccccgccgcg gcctgagagc catccacacg
     4681 ggcgcacagg agctgcgcgc ttgcgcacgg tctgcgcaaa acacaatgag ggccgctcgg
     4741 agtgggcctg agacttcgtc acgcgctcac ccgccgctcg ccgccacgct gggcctgcgg
     4801 ttccctctcc ctcaatagcg caggaacacc gagccccagg tcaggccggc gccgaacgcg
     4861 accaggcaga ccatgtggcc gggctggatc ttgccgagcg cgatcgcctc gtgcatgcag
     4921 atcgggatcg aggcggcggt ggtgttgccg tacttctgga tgttgttgtg catcttctcc
     4981 ggcggcagcc cgatcatctg cccgaccatc tggttgatcc gcaggttcgc ctggtgcggg
     5041 atcatcatgt cgatgtcggt cagcttcagg ttgttggtga ccatgccctc catgatcgtc
     5101 gccggcatcc gggtcaccgc gtgcttgaac acgcgcttgc cgttcatcac cgggtagtgc
     5161 ttgcgctcgg ccatcgcctc ggcggagatc cgcgggtggt gggcgctcgc ggggtactcg
     5221 gtccacagga tctcggcctc gctgccgtcg gcgtggatat gggtcgacag gatcatgtgc
     5281 gactcgtcgg tcgcgcggcc gacgaccacg gccccggcgc cgtcgccgaa cagcacggtc
     5341 acgtcgcgcc ccgcggtcga ggtgtcgagc ccggtcgagt ggacctcggc gccgatcagc
     5401 aggatgtgct tgaacgcgcc ggtgcggatg tacgcatcgg cgatcgacat cccgtagatg
     5461 aaaccggtgc actgctgccg gatgtcgatg cacgggatgt cgcgcaggcc gagcgcgcgc
     5521 tggacgaaca cgccggtgcc ggggaagttg aagtccggcg acagcgtcgc gtagatgatc
     5581 atgtcgatat cgcgcggcga caggccggcg cgctcgatcg cctccttgct cgccgcggtg
     5641 gccagcgtcg cgccggtctc gccctcggac acccaccgcc gctcctcgat gcccgtgcgc
     5701 tcgatgatcc actcgttcga cgtctccatc atccgtgcga gatcgtgatt ggtgacgacg
     5761 cgcgggggca cgctcatgcc gatgcccagc aagcggctgc gatgaagcat tgccatgggc
     5821 taccacagcc cggtgcgcgc aagcgagtca tcgtgcagat ccacgacggt gtgtgaacag
     5881 gctttcgcac cggcgcacgg cgtgcgcacg agaatgtcgt agcggtccgg ccgtcggagc
     5941 tcgtctgccc cgtaactcgc tgcgtcagga gggtcgtcgg tcagacgtcg aggttgcgga
     6001 cgttgagcgc gttctcctcg atgaactcgc ggcgcggctc gacgagatcg cccatcagct
     6061 tggtgaagat gccgtcggcg gcctccatct cctcgacctt gaccttgagc aggttgcgct
     6121 tggcgggatc catcgtggtc tcccacagct gctcggcgtt catctccccg aggcccttgt
     6181 agcgctggat ctgcaatccc ttgcggccga gctcctcgat cacggtcgcg atctcgtccc
     6241 agctctcgat cgcgcgcgcc tcgccctcct cgtgctggat cgacagcggc tcggtcagcg
     6301 tggccttgag ctcggcgctc gcgcgccgca gctcgacgaa ctccgcggag cgcacgacgt
     6361 cggagttgat caccgtggcg cgccggatcc cgtgcgcgcc ggccgggaac cggatctccc
     6421 aggtgccgtg ctcgatgtcc tgcttgtacg tcgccgccgc ctggccgagc tcgccgtggc
     6481 ggcgcgacac ctcggccatg accttggcct cgagccgctc gagcttgtcg cggtccttga
     6541 ggtcgtcctc ggacagcccg gcctcggcga actgcgcggt gatccggccg tcgccgcgct
     6601 tgtcgagctg ctcgcgcagc cggcgcgcct ggctgatccg cttgaccagc gcgacgagct
     6661 cgtggccggc gagcggcgag gagtcggagc cgcgatgcag cacggtctcg cggcacacgc
     6721 tgtcgagcag gtagtcctcg agcgcgatct cgttcttgag gtacagctcg gtcttgccct
     6781 tcttgacctt gtagagcggc ggctgcgcga tgtagacgtg gcccttctcg aacagctcgt
     6841 tgaagtggcg gaagaagaac gtcagcagca gcgtccggat atggctgccg tcgacgtcgg
     6901 cgtccgtcat gatgatgatc ttgtggtagc gcagcttgtc gaggtccttg gcgtcgccga
     6961 cgccggtccc gagcgcggtg atcagcgtcg tcagctcctg cgacgacagc atgcgatcga
     7021 accggacctt ctcgacgttc aagatcttgc cgcgcagcgg gaggatcgcc tggaactgcc
     7081 ggctgcggcc ctgcttggcc gagccgccgg cgctctcgcc ctcgacgatg aagatctcgc
     7141 tcttctcggg gtcgcgctcc tggcagtcgg cgagctt
//
LOCUS       DASVOR010001257         2725 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_334163,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001257 DASVOR010000000
VERSION     DASVOR010001257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2725
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334163"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..653
                     /gene="nuoB"
                     /locus_tag="VF516_24025"
     CDS             108..653
                     /gene="nuoB"
                     /locus_tag="VF516_24025"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HEX8110830.1"
                     /translation="MGLELTTTRLEDAAKWAKQSALDRASNWGRKFSLFTYPFVTACC
                     GMEFMSVAAPKYDLARFGAEFPRFSPRQSDLLMIVGTITEKQGPALKRVYDQMAEPKW
                     VIAFGVCASTGGFYQNYHAMPGADQVIPVDVYIPGCPPRPEQVLDALIMLMDRIQSGR
                     GHSQLRNKREQLIAHAPANEG"
     gene            663..1244
                     /locus_tag="VF516_24030"
     CDS             663..1244
                     /locus_tag="VF516_24030"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEX8110831.1"
                     /translation="MSAKVLDALKAAHGGAIDSVESQHGDAIAWIKRDQLLAVATWLK
                     NDPKMAFDSPVFCTCIDRLDWRPVDVPPGPPSYPTTAAWTEDHPRFEVVYQLRSLPHR
                     HRIRLKCAVPEHDARVPSLATLWPAFNWQERETYDMYGIKFDGHPDLRRIYLYEEFVG
                     HPLRKDYPKEKRQPLVRRDDLPINPIKAPGGHR"
     gene            1244..2542
                     /locus_tag="VF516_24035"
     CDS             1244..2542
                     /locus_tag="VF516_24035"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HEX8110832.1"
                     /translation="MAAPSSSSSSKSNEPGRSLKQLVLGRRDVQDLSDNEQELPSELM
                     TVNMGPSHPAMHGTVRIVLTVDGERVVASDVQPGYLHRCFEKESEHATYTQVFPYTDR
                     LNYVSPMINNVGWAMAVEKLMGLTGQIPRRAEYIRVIVSEMSRMTDHLTCVGASAMEL
                     GAFTPFLYLLKCREWLYELLEKVSGARLTHSYVRIGGVAKDLTPGFGGDDGELKLCLK
                     QIAKVMVEVENLLNHNKIFRDRMAGIGAMSKDDAIATGCVGPIARAAGVDYDVRKDYP
                     YSIYPEVAFDVPVGTTGDCYDRYLVRIEELKQSSRILDQCLDQIPDGPVMVDDPRVAL
                     PAKAATYNTIESMIRHFKHIFDGIKVPAGEAYAFVEGGNGELGFYIVADGTGRPYKAY
                     VRSPSFVHLSTASQLIHGHLIADIVPIFGMINMIGGECDK"
     gene            2539..>2725
                     /locus_tag="VF516_24040"
     CDS             2539..>2725
                     /locus_tag="VF516_24040"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8110833.1"
                     /translation="MTDVADSVTLTIDGMSVTVPKGTNVLEAARTLGIDISAFCYHPG
                     LPVVAQCRQCLVSVEKNP"
BASE COUNT          471 a          922 c          897 g          435 t
ORIGIN      
        1 gcgcccggaa tcttcctggt gagcgtgttc gggttcgtcg agatgctggt gttcgtcgcg
       61 atcctcgtcg tcgcgctcgc ctacgtctgg agaaagaagg cgatcggatg ggactagagc
      121 tgaccaccac gcgcctggaa gacgcggcca agtgggcgaa gcagagcgcg ctcgaccgcg
      181 cgagcaactg ggggcgcaag ttctcgctgt tcacgtaccc gttcgtcacg gcgtgctgtg
      241 gcatggagtt catgtcggtc gccgcgccca agtacgacct cgcgcggttc ggcgccgagt
      301 tcccgcggtt ctcgccgcgc cagagcgacc tcttgatgat cgtcggcacg atcaccgaga
      361 agcaggggcc cgcgctcaag cgggtctatg accagatggc cgagcccaag tgggtgatcg
      421 cgttcggggt ctgcgcctcg accggcgggt tctaccagaa ctaccacgcg atgccgggcg
      481 ccgatcaggt catccccgtc gacgtctaca tcccgggctg cccgcctcgc cccgagcagg
      541 tgctcgacgc actgatcatg ctcatggacc gcatccagtc cggccgcggc cacagccagc
      601 tgcgcaacaa gcgcgagcag ctgatcgcgc acgcgccggc gaacgaaggc tagaggctcg
      661 ctatgtcggc aaaggttctc gatgcgctga aggccgcgca cggcggcgcg atcgactcgg
      721 tcgagtcgca gcacggagac gcgatcgcct ggatcaagcg cgaccagctg ctcgcggtcg
      781 cgacctggct gaagaacgat cccaagatgg cgttcgattc gccggtgttc tgcacctgca
      841 tcgaccggct cgactggcgg ccggtcgacg tcccgcccgg tccgccgtcg tacccgacca
      901 ccgcggcgtg gaccgaggac cacccgcggt tcgaggtcgt ctaccagctg cgctcgctcc
      961 cgcatcgcca ccggatccgg ctcaagtgcg ccgtccccga gcacgacgcc cgcgtgccgt
     1021 cgctggccac gctgtggccg gcgttcaact ggcaggagcg cgagacctac gacatgtacg
     1081 ggatcaagtt cgacggtcac cccgacctgc ggcggatcta cctgtacgag gagttcgtcg
     1141 gccacccgct gcgcaaggac taccccaagg agaagcgcca gccgctggtg cggcgcgacg
     1201 acctgccgat caacccgatc aaggcaccgg gagggcaccg ctgatggccg ctccgtcttc
     1261 ctcttcatcc agcaagtcga acgagcccgg ccggtcgctc aagcagctcg tgctcggccg
     1321 ccgcgacgtc caggatctgt cggacaacga gcaggagctg ccgtccgagc tgatgacggt
     1381 caacatggga ccgtcacacc cggcgatgca cggcacggtg cggatcgtgc tgacggtcga
     1441 cggcgagcgt gtcgtcgcgt ccgacgtcca gcccgggtac ctgcaccgct gcttcgagaa
     1501 ggaatccgag cacgcgacct acacccaggt gttcccgtac accgatcgcc tgaattacgt
     1561 ctcgccgatg atcaacaacg tcggctgggc gatggcggtc gagaagctga tggggctgac
     1621 cggccagatc ccgcggcgcg ccgagtacat ccgcgtgatc gtcagcgaga tgtcgcggat
     1681 gaccgatcac ctgacctgcg tcggcgcgtc ggcgatggag ctcggcgcgt tcaccccgtt
     1741 cctctacctg ctcaagtgcc gcgagtggct gtacgagctg ctcgagaagg tgtcgggcgc
     1801 gcggctgacc cacagctacg tacggatcgg cggcgtcgcc aaggacctga cgcccggctt
     1861 cggtggtgac gacggtgagc tgaagctgtg cctcaagcag atcgccaagg tgatggtcga
     1921 ggtcgagaac ctgctcaacc acaacaagat ctttcgcgat cgcatggccg ggatcggcgc
     1981 gatgtcgaag gacgacgcga tcgccaccgg ctgcgtcggg ccgatcgcgc gcgcggccgg
     2041 cgtcgattac gacgtgcgca aggactaccc ctactcgatc tatcccgagg tcgcgttcga
     2101 cgtgcccgtc gggaccaccg gcgattgcta cgaccgctac ctggtgcgca tcgaggagct
     2161 caagcagtcg agccggatcc tggaccagtg cctggatcag atccccgacg gcccggtcat
     2221 ggtcgacgat ccgcgggtcg cgctgccggc gaaggcggcg acctacaaca cgatcgagtc
     2281 gatgatccgg cacttcaagc acatctttga cggcatcaag gtgccggccg gcgaggccta
     2341 cgcgttcgtc gagggcggta acggcgagct cgggttctac atcgtcgccg acggcaccgg
     2401 ccggccgtac aaggcctacg tccgctcgcc gtcgttcgtg cacctgtcga cggcgagcca
     2461 gctgatccac ggccacctca tcgccgacat cgtcccgatc ttcggcatga tcaacatgat
     2521 cggcggggag tgcgacaagt gacagacgta gcagactccg tgacgctgac gatcgacggc
     2581 atgtcggtca ccgtcccgaa gggcaccaac gtgctcgagg ccgcgcgcac gctcggcatc
     2641 gacatcagcg cgttctgcta ccacccgggg ctgccggtgg tcgcgcagtg ccggcagtgc
     2701 ctggtgtcgg tcgagaagaa cccca
//
LOCUS       DASVOR010001258         4765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363498,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001258 DASVOR010000000
VERSION     DASVOR010001258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4765
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363498"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VF516_24045"
     CDS             complement(<1..359)
                     /locus_tag="VF516_24045"
                     /inference="COORDINATES: protein motif:HMM:NF028174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase dimerization domain-containing
                     protein"
                     /protein_id="HEX8110834.1"
                     /translation="MWNINTIDDDIKVPPKTAAQVYLWARNTLDKLKRAITPPQLHVF
                     ELLDGHKVTQCLCVAMRLGVADQLNNGPKACSELARALHVNADALYRVMRLLASKQIF
                     KELPDRRFELTPAAALLR"
     gene            1066..1233
                     /locus_tag="VF516_24050"
     CDS             1066..1233
                     /locus_tag="VF516_24050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110835.1"
                     /translation="MYAHLAPEAWHQDYHRLAFHVPGESARVFEIVRHDRRTLAAHER
                     EPHNQPACSGC"
     gene            complement(1230..3647)
                     /locus_tag="VF516_24055"
     CDS             complement(1230..3647)
                     /locus_tag="VF516_24055"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX8110836.1"
                     /translation="MTDHAVGLGFDETLEGGFAMAATDPYGGEKLGKARGSSLTLRNH
                     ITIASVDEFIADQDHRAVLHAQIDFAPLGTGIQCTPGIFQLFPQSDDPAVKLMVYACG
                     FTAGGARYYLEGKKLIHEGEPLHTLLDQTTTLYTRLHAGSDATGPVVGAGVLRIGVPG
                     VIELVASIRTTGATSVEQSARAIIAFGAFFFGELWESYAGHVSGREALLVQVLSSSRA
                     VPSVRPPVTPRPRLALPAADLPDDATATVVVVGSGYGGGIAASRLARTGHKVIVLERG
                     KEWRAGDFPTGPAEAALQLQVDGPSGRRGPRTGLFDLHNNAELDVLVGCGLGGTSLIN
                     ANVSLEPAAEVFDDPRWPKALRDDRARLADSYARARAMLAPVPYPDDAPPLHKLQVLE
                     READALHRPFMRPPINVVFSDRINAGGVAQSACRLCGDCVTGSNYNAKTTVDMSFLPD
                     AKAHGAELYTEVTVRWIEQAGDQFRVKWRWTEAHDDDALRTITADVVVLAAGTLGSTE
                     ILLRSAEHGLAVSPRVGHQLSANGDYVSFGYNKDTTVHAVGAGNRPLARIGPVGPTVT
                     GMIGPRAGQSLPEQYIIQEAVIPGALADALPLAFTVAASAFGVNTATSKEARRQQAAR
                     ALESLAAGRAGPYVGAVNHTQTFLTIGHDSGAGTMALVDDRLRISWPGVGGQPIYQEM
                     QETTTAATAVTQGISIREPIWTDVFDDNMITVHPLGGCVMGDDAQHGVVNDRGQVFSG
                     SSGTAVYHGLYVADGAVVPTSLGVNPLLTICALAERAMTLLIRDRGWTASRRAQGASR
                     RSGRLDG"
     gene            4129..4260
                     /locus_tag="VF516_24060"
     CDS             4129..4260
                     /locus_tag="VF516_24060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110837.1"
                     /translation="MRWFLAGYIVCPLLLGLLVAIGEPHVRSAPMNWFNFYDFWSPS"
     gene            4257..>4765
                     /locus_tag="VF516_24065"
     CDS             4257..>4765
                     /locus_tag="VF516_24065"
                     /inference="COORDINATES: protein motif:HMM:NF012447.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX8110838.1"
                     /translation="MTVLRIGEARVTHSDVARVARGELTVALTEAASWHDRMRASRRA
                     VDARVQSGEPFYGVNTGFGASVVNTVAAEHGGNLAVNLFRYHGCGVGPALGPDEVRAV
                     IVARLAQLAAGWSGIRVETLRAMVALLEHEVLPVIPELGSVGASGDLTPMSYVAAVLS
                     GEREVSVAGR"
BASE COUNT          711 a         1627 c         1652 g          775 t
ORIGIN      
        1 cgcagcagag ccgccgccgg ggtcaactcg aagcgccgat ccggcagctc cttgaagatt
       61 tgcttgctgg cgagcagccg catcacgcgg tagagcgcgt ccgcgttgac gtggagcgcc
      121 ctggccaact cggagcacgc cttggggccg ttgttcagct gatcggcgac cccgagccgc
      181 atcgcgacgc acagacactg tgtgaccttg tggccgtcga gcaactcgaa cacgtgcagc
      241 tggggcggcg tgatcgctcg cttcagcttg tcgagggtgt tacgcgccca gagatagacc
      301 tgcgccgcgg ttttcggtgg caccttgatg tcgtcatcga tggtgttgat gttccacatg
      361 cccactctcc gtcgcctcga ttgtgtcgct attgcgaagc ttcgcataca tacgcgtgac
      421 cggagactcg tgcgccaccc tcacagggca tcatcagtga ttgcacgacg ctgtgagaac
      481 tctagtagcc gttgattata tgtcaacgat gatattcgtt agcgagccat gatgcagcga
      541 cgcgctgtag gacgcgagcc gtcatgctgc gatacgttgc acgtccgagc agggacgacc
      601 ggcaagcctc gagggagagc tggcggcaca atggaaggcg ctgtccccgc cacagcgtcg
      661 aaggagcccg tgcgcgccaa agcccacctg gcatcccgga tcggggtaca gcgatcctga
      721 tgggcacttc gagaggtgaa atgccttgtc cgatacatgg ctgtgatctt cgcaaccgac
      781 cacgaccttg ggagcaactc tcatacgccg tcagcactcc tgcaggcgga tcgcgcgatt
      841 cgcgacaatg gtgatgcccc ggaggaccgg acgggaccca cggtcatgcg ggtcggcgag
      901 tcggaagcgg cgacacgcgg accataccct tgtgatcccg ccccgccatg cttccgagcg
      961 acttgaccga gaagttttcc caccagttcc ggatctgccg cggcatgaag tagcccgagg
     1021 tcccggaccg gcatacaacc ggatccggac gagggccgat tctggatgta cgcccacctc
     1081 gcgcccgagg cgtggcatca ggactaccac cggctcgcgt tccacgtgcc cggcgagtcg
     1141 gctcgggtgt tcgagatcgt gcgtcatgac cgccgcacgc tggcagctca cgagcgcgag
     1201 ccacacaatc agccagcgtg ctccgggtgc tagccgtcga gccgcccgct ccggcgactc
     1261 gcgccctgcg cgcggcgact cgcggtccag ccgcggtcgc ggatcagcag cgtcatggcg
     1321 cgctcggcga gcgcgcagat cgtgagcagc gggttgacgc ccagcgacgt cgggacgacc
     1381 gcgccgtcgg cgacgtagag cccgtgatac acagctgtgc ccgacgagcc agagaacacc
     1441 tggccgcggt cgttgaccac gccgtgctgg gcgtcatcgc ccatcacgca gccgccgagt
     1501 gggtgcaccg tgatcatgtt gtcatcgaac acgtcggtcc agatcggctc ccggatcgag
     1561 atgccctggg tcaccgcagt ggcggccgtg gtggtctcct gcatctcctg gtagatcggc
     1621 tggccgccca cgcccggcca cgagatccgc agccggtcgt cgacgagcgc catggtgccg
     1681 gcgccgctgt catggccgat cgtcaggaac gtctgggtgt gattgaccgc gccgacgtag
     1741 gggccggccc ggcccgcggc cagactttcg agtgcgcggg cggcctgctg gcggcgggcc
     1801 tccttgctgg tcgcggtgtt gacgccgaac gcgctggccg caacggtgaa cgccagcggg
     1861 agcgcgtcgg cgagcgcgcc tggaatcacg gcttcctgga tgatgtactg ctcgggcagc
     1921 gactgtccgg cccgcggacc gatcatgccg gtgaccgtcg ggccgaccgg gccgatccgg
     1981 gccagtgggc ggttgccggc accgaccgcg tggacggtgg tgtccttgtt gtagccgaag
     2041 ctgacgtagt caccgttggc gctgagctgg tggccgaccc ggggcgatac cgcgaggccg
     2101 tgctccgccg agcgcagcag gatctcggtc gagccgagcg tgcccgcggc gagcaccacg
     2161 acgtcggcgg tgatcgtgcg cagcgcgtcg tcgtcgtggg cttcggtcca tcgccacttg
     2221 acgcggaact ggtccccggc ctgctcgatc cagcgcaccg tgacctcggt gtagagctcc
     2281 gcgccgtggg ccttggcgtc gggcaggaac gacatgtcga cggtggtctt cgcgttgtag
     2341 ttgctgccgg tcacgcagtc gccgcacagc cggcatgccg actgtgcgac gccgccggcg
     2401 ttgatccggt cgctgaacac gacgttgatc ggtgggcgca tgaacggccg gtggagcgcg
     2461 tcggcctctc tctccaggac ctggagcttg tgcagcgggg gggcatcgtc gggatacggc
     2521 accggcgcga gcatcgcccg ggcgcgggcg tagctgtcgg cgagccgcgc gcggtcatcg
     2581 cgcagcgcct tgggccagcg cgggtcgtcg aacacctcgg ccgccggctc cagggatacg
     2641 ttggcgttga tcagcgacgt gccgcccagg ccgcagccga ccaggacgtc gagctcggcg
     2701 ttgttgtgca gatcgaacag cccggtgcgc gggccgcgcc ggccgctcgg gccgtcgacc
     2761 tgcagctgca gggcggcctc cgcaggcccg gtcgggaagt caccggcgcg ccactccttg
     2821 ccgcgctcca gcacgatcac cttgtggccg gtccgcgcca gccggctcgc tgcgatcccg
     2881 ccgccgtatc ccgagccgac cacgaccacg gtcgcagtcg cgtcgtcggg gaggtcggcg
     2941 gcgggaagcg ccagccgcgg ccgcggcgtg acggggggcc ggacgctggg gacggcccgc
     3001 gacgacgaca gcacctggac cagcagcgcc tcgcgtccgg acacgtggcc ggcatagctc
     3061 tcccacaact caccgaagaa gaatgcgccg aacgcgatga tcgcgcgcgc ggactgctcg
     3121 acgctggtcg cgccggtcgt gcgaatcgag gccacgagct cgatcacgcc gggcacgccg
     3181 atgcggagca cacccgcccc caccaccggg ccggtcgcgt cgcttccggc atggagccgg
     3241 gtgtacagtg tggtggtctg atcgagcagc gtgtgcagcg gctcaccctc gtggatgagc
     3301 ttcttgccct ccaggtagta gcgcgcgcct ccggcggtga agccgcaggc atagaccatc
     3361 agcttgaccg ccgggtcgtc gctctgaggg aacagctgga agatccccgg ggtgcactgg
     3421 atcccggtgc cgagcggcgc gaagtcgatc tgggcatgga gcaccgcgcg gtgatcctga
     3481 tcggcgatga actcgtcgac gctcgcgatc gtgatgtggt tgcgcagggt cagtgagcta
     3541 cccctggcct tgccgagctt ctcgccgccg tatgggtcgg ttgcagccat tgcgaagccg
     3601 ccttccagcg tctcgtcgaa tcccaggcct accgcgtgat ctgtcatgcc cccctcctcc
     3661 agagaatgaa cagaccacga taggatagat tgatcggcgc ggcgggcggc tgccggacta
     3721 tgccgcggtg ccgcgcgcga tcgcatcgcg ggtcggcccg ggctgaccgg accacgatga
     3781 cagaatacgg atcctcgcgc gctggcgcac atcgtcaccg cgggcggctc gcgcggcgct
     3841 ccggcgctgt gctccgctgt acgtttcatg cggcagctcg cttgccgccg aacgtggatt
     3901 ccacctcgcg gtgggccgag tcggtcgtcg cccaaggccg aagtcgtcat tgtggtcgac
     3961 cagcggaact ggctcgcgcc gaagtcactt atctgtttac agcccgactc gcgatcggcg
     4021 gcgaattccg gaaaatcgct ctcctgtgga cgaccgaacc cgcggcgctc tgcgccgcgc
     4081 tatactcgcg ccaatgagcc gaatcctgcc tgaagtgagg tggatcctgt gaggtggttc
     4141 ctggccggat atatcgtctg cccgctcctc cttggactcc tggttgccat cggggagccc
     4201 cacgtgcgct cggcgccgat gaactggttc aacttctacg acttctggag cccgtcgtga
     4261 ccgtgctgcg catcggcgag gcccgcgtca cccacagcga cgtcgcgcgg gtcgcccgcg
     4321 gcgagctcac cgtcgcgctg accgaggccg cgagctggca cgaccggatg cgcgccagcc
     4381 gccgcgcggt cgacgcgcgc gtgcagagcg gcgagccgtt ctacggcgtc aacaccgggt
     4441 tcggcgcctc ggtcgtcaac accgtcgccg ccgagcacgg cggcaacctc gccgtcaacc
     4501 tgttccggta ccacggctgc ggcgtcggcc cggcgctcgg tcccgacgag gtccgcgccg
     4561 tgatcgtcgc gcggctcgcc cagctcgcgg cgggctggag cggcatccgg gtcgagaccc
     4621 tgcgcgcgat ggtcgccctg ctcgaacacg aggtcctgcc cgtgatcccc gagctcggct
     4681 cggtcggcgc gagcggcgat ctcacgccga tgtcgtacgt cgccgcggtg ctgtcgggcg
     4741 agcgcgaggt ctcggtcgcc ggccg
//
LOCUS       DASVOR010001259         3709 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_451110,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001259 DASVOR010000000
VERSION     DASVOR010001259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451110"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..606)
                     /locus_tag="VF516_24070"
     CDS             complement(127..606)
                     /locus_tag="VF516_24070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110839.1"
                     /translation="MTSGVSGPAAGYLPHQPPMLLIDDIVEVTEHRAVCRATIHPDCV
                     FAIDGAVHPAAMIEFVAQACAIYAGAMSAQDGGPPRLGLIMACREAAFAVDAFAVGDE
                     LTIVAEKAFGGNQMASFTGTVSRGDALCATIQLSVVDAELAALSPAGGGARDLEGEP"
     gene            complement(603..1388)
                     /locus_tag="VF516_24075"
     CDS             complement(603..1388)
                     /locus_tag="VF516_24075"
                     /inference="COORDINATES: protein motif:HMM:NF025100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HEX8110840.1"
                     /translation="MTPGTTVGPIQIRKTAFFSTCHASAQDVLAGRERPFTAPAYGLL
                     VGRARRFTSLVTQMHVEVCGALQPEAHRAPAVFATCHGEIQTCERLIADFRDTSMVSS
                     ARFALSVHNTASGVYSVATDSTAPTTTVTGANAIAAGWLEAVLIALDAERPVLLSIAD
                     EPVPAVFRGPTEPVGFAAAFLVGAAPAPADSGCTAELAVAACPDGPEGDLTIDPLRTL
                     ARLAEAWAQRAHVTIALGPIQPGAMLELRVWPPGPPAPGEVRA"
     gene            complement(1385..2680)
                     /locus_tag="VF516_24080"
     CDS             complement(1385..2680)
                     /locus_tag="VF516_24080"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8110841.1"
                     /translation="MPDRSGRPHPRPVVTTHCAITAAALANCLGTDSRTVLDRAFAGV
                     DAFSPAGAFHEFPFPTMLGVMPGIEEHRGLRAAERPPADGDPLDCISTRLAMAALPSV
                     RQLRPAVHAAVERWGARRVAYVFASSTGGLDETERALAPDPRLPLASACYHYADHGVD
                     ATSQAIARSFGALGVCLAISTACSSSFHALASALRLIDRGLADAAVVGGADSLCRTTV
                     FGFHGLGLTAPTATRPFARERAGITIGEGSAYALVERDAPDTRRRALAWIAGIGAASD
                     AFHHTSPHPDGLGGQLCMRQALDRAGMSPDAIDCVSAHGTGTKLNDAAEAAAVHQVFG
                     RRVPLTATKSLTGHTLGSAGLTALVLAVESLRRQAIPATLRAAPLDEALGVDVVDRLT
                     AAELRHVLVNAFGFGGSNVSVVVSRPDLRPDLRTTTRPS"
     gene            complement(2638..>3709)
                     /locus_tag="VF516_24085"
     CDS             complement(2638..>3709)
                     /locus_tag="VF516_24085"
                     /inference="COORDINATES: protein motif:HMM:NF016693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEX8110842.1"
                     /translation="VLAGGFQFKNGATFVCDELYSEFNFANKFTAGCSFTFQVLRSEF
                     DHLLANEAERAGATVQYGVEITAVDVGGERPCVTTRTEDGDVVVHRPRFVLDASGFGR
                     TLARLLDLEKPSSFPVRAARFCHVKDGIVAGAFDRQKIRIGINPTNRDVWSWLIPFPN
                     GTSSLGIVAARDHLARYTGSDEDRFWAMVGDEPKLRILLEHAHTVRPIGELIGYAANV
                     KTLHGPGFALLGNAAEFLDPVFSSGVTIALHSAKLAAAALDRQLRGEPVDWQYEFAEP
                     LMFGIETFRTFVNGWYDGSLQDVIFYPDKVPAIHRMICSVLAGYAWDPANPYTGPQSA
                     RRLRVLAAACRTGAAGPTPGPS"
BASE COUNT          522 a         1313 c         1404 g          470 t
ORIGIN      
        1 cggcggcgcg ctggtggacg caggtcagcg cgacgtcgag gccgtcccgg gccagggtcc
       61 gtgcgatcgc cgcgccgatc ccgcggctcg ccccggtgac cagcgcgacc ttgtgcggga
      121 tcgcggtcat ggctcgccct ccaggtcccg ggcgccgccg ccggccggcg acagcgcggc
      181 gagctcggcg tcgacgaccg acagctggat cgtcgcgcac agcgcgtcgc cgcggctgac
      241 cgtgccggtg aacgaggcca tctggttgcc gccgaacgcc ttctcggcga cgatcgtgag
      301 ctcgtcgccg accgcgaacg cgtcgaccgc gaacgcggcc tcgcggcacg ccatgatcag
      361 gccgagccgc ggcgggccgc cgtcctgggc cgacatcgcg ccggcataga tcgcgcaggc
      421 ctgcgcgacg aactcgatca tcgcggcggg gtggaccgcg ccgtcgatcg cgaacacgca
      481 gtcggggtgg atcgtcgcgc ggcacaccgc gcggtgctcg gtgacctcga cgatgtcgtc
      541 gatcagcagc atcggcggct ggtgcgggag gtagcccgcc gccgggccgg acacgccgga
      601 ggtcacgcgc gcacctcccc gggcgccgga ggacccggag gccagacgcg gagctcgagc
      661 atcgcgccgg gctggatcgg gccgagcgcg atcgtcacat gtgcgcgctg cgcccaggcc
      721 tcggccaggc gtgccagcgt ccgcagcggg tcaatcgtca ggtcaccctc cgggccgtcg
      781 gggcaggccg cgaccgcgag ctccgccgtg cagccggagt ccgccggggc cggtgcggcg
      841 ccgaccagga acgccgcggc gaagccgacc ggctcggtcg ggccgcggaa caccgccggg
      901 accggctcgt cggcgatgct gagcaggacc gggcgctcgg cgtcgagcgc gatcagcacc
      961 gcctcgagcc agcccgccgc gatcgcgttg gcgccggtga ccgtcgtcgt cggcgcggtg
     1021 ctgtcggtcg cgaccgagta cacccccgaa gcggtgttgt ggacgctgag cgcgaagcgg
     1081 gcggagctca ccatcgaggt atcgcgaaag tcggcgatca gccgctcgca ggtctggatc
     1141 tcgccgtggc aggtcgcgaa cacggcgggc gcgcggtgag cttctggctg cagcgcgcca
     1201 cagacctcga catgcatttg cgtgaccagg ctggtgaacc ggcgagcgcg gccgacgagc
     1261 agcccgtagg ccggcgctgt gaacgggcgt tcacgtccgg cgagcacatc ctgggcggag
     1321 gcgtgacacg tcgagaagaa cgccgtcttc cggatctgga tcggcccgac cgtcgttccc
     1381 ggtgtcatga cggcctcgtg gttgttcgca gatccggtcg cagatccggt cgcgacacga
     1441 cgacgctcac gttgctgccg ccgaacccga acgcgttgac cagcacgtgg cggagctcgg
     1501 ccgcggtcag ccggtcgacc acgtcgacgc cgagcgcctc gtcgagcggc gccgcgcgca
     1561 aggtcgccgg gatcgcctgg cgccggagcg actcgacggc gagcaccagc gcggtcagcc
     1621 ccgccgatcc gagcgtgtgg ccggtcaggc tcttggtcgc ggtcagcggg acgcggcggc
     1681 cgaacacctg gtgcacggcc gctgcctcgg ccgcgtcgtt gagcttggtg ccggtgccgt
     1741 gcgcgctgac gcagtcgatg gcgtcgggcg acatcccggc gcgatcgagc gcctgccgca
     1801 tgcagagctg gccgccgagt ccgtcggggt gcgggctggt gtggtggaac gcgtcggacg
     1861 ccgcgccgat cccggcgatc caggccagcg cccgccgccg ggtgtccggc gcgtcgcgct
     1921 cgaccagcgc gtaggccgag ccttcgccga tcgtgatgcc cgcgcgctcg cgcgcgaacg
     1981 gccgggtcgc ggtcggcgcg gtcagcccga ggccgtggaa cccgaacacc gtggtgcggc
     2041 acagcgagtc ggcgccgccg accacggcgg cgtccgccag gccgcgatcg atcagccgga
     2101 gcgcggacgc cagcgcgtgg aagctcgacg agcaggcggt cgagatcgcc aggcacacgc
     2161 caagggcccc gaagctgcgc gcgatcgcct ggctggtcgc gtcgaccccg tgatcggcgt
     2221 agtgatagca cgcgctcgcc agcggcagcc gcggatccgg tgccagcgcg cgctcggtct
     2281 cgtcgaggcc gccggtcgag ctcgcgaaca cgtaggcgac gcggcgcgcg ccccagcgct
     2341 cgaccgcggc gtgcacggcg ggccggagct ggcgcaccga gggcagcgcc gccatcgcga
     2401 gccgggtcga gatgcagtcg agcggatcgc catccgcggg ggggcgctct gcggcgcgca
     2461 ggccgcgatg ctcctcgatc cccggcatca cgccgagcat cgtcgggaac gggaactcgt
     2521 ggaacgcgcc ggccggcgag aacgcgtcga cgcccgcgaa cgcccggtcc agcaccgtgc
     2581 ggctgtccgt cccgaggcag ttcgcgagtg ccgcggcggt gatggcgcag tgggtcgtca
     2641 cgacgggcct ggggtggggc cggccgctcc ggtccggcac gcggcggcca gcacacgcaa
     2701 ccggcgcgcg gactgcgggc cggtgtaggg gttggccgga tcccaggcgt acccggcgag
     2761 caccgagcag atcatgcgat ggatcgcggg gaccttgtcc ggatagaaga tcacgtcctg
     2821 aaggctgcca tcgtaccagc cgttgacgaa cgtgcggaac gtctcgatcc cgaacatcag
     2881 cggctcggcg aactcgtact gccagtcgac cggctcgccg cggagctggc gatcgagcgc
     2941 ggccgccgcg agcttggccg agtgcagcgc gatggtgacg cccgacgaga acaccgggtc
     3001 gaggaactcg gcggcgttgc cgagcagcgc gaaccccggg ccgtgcagcg tcttgacgtt
     3061 ggccgcgtag ccgatgagct cgccgatcgg ccgcaccgtg tgcgcgtgct cgagcaggat
     3121 ccgcagcttg ggctcgtcgc cgaccatcgc ccagaaccgg tcctcgtcgc tgccggtgta
     3181 gcgcgcgagg tggtcgcggg cggcgacgat gccgagcgag ctcgtgccgt tggggaacgg
     3241 gatcagccac gaccacacgt cgcggttcgt cgggttgatc ccgatccgga tcttctggcg
     3301 gtcgaacgcc ccggcgacga tgccgtcctt gacgtggcag aaccgggcgg cgcggaccgg
     3361 aaagctcgac ggcttctcga ggtcgagcag ccgcgccagc gtgcggccga acccgctggc
     3421 gtcgagcacg aaccggggcc ggtggaccac gacatcgccg tcctcggtcc gggtcgtgac
     3481 acagggccgc tcaccgccga catcgaccgc cgtgatctcg acgccgtact gcacggtcgc
     3541 tccggcgcgc tcggcctcgt tggccaggag gtggtcgaac tccgagcgca gcacctggaa
     3601 cgtgaacgag cacccggcgg tgaacttgtt ggcgaagttg aactccgagt acagctcgtc
     3661 gcacacgaac gtggcgccgt tcttgaactg gaacccgccg gcgagcacg
//
LOCUS       DASVOR010001260         3878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_305220,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001260 DASVOR010000000
VERSION     DASVOR010001260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305220"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1477)
                     /locus_tag="VF516_24090"
     CDS             complement(134..1477)
                     /locus_tag="VF516_24090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HEX8110843.1"
                     /translation="MAGESTGSPELAGGGGATGGSGTGASKLVSVTNLTADQSGKAAS
                     VESSLVNAWGIVGFQGWFWITDNATGKLSILDAAGHPAGAQQGGTGQGQGQGQGQGQG
                     QGTLNVASSSIDLGEGITGIAVNPSNAMQMDGKGANAQACGPANLIVASETGPLIGVN
                     TSLNTTGGQVLVDRSKVGASYTGVAVLDVKAGATCSATSGTCDGSMGSGTQGNGQGMG
                     SAGTGTGSGNAGTGTGMGNGGMGNAPQPGVLVLAADFHNARIDVFDEKFNLVTTPMFS
                     TPASVRAGFAPFNVAVFNGIVYVTYAQQNPEKTDSVAGAGLGFVVAFDLCGNVLWTSS
                     GAQFNAPWGMALGVDLSLAPGVLLVGNFGDGAITEVNPKDGSIIDQLMTTAGAPLKID
                     GLWGLAIGTGSGVQSVQAAGVYFVSGPQDEAHGLFGVITLAAAAPGSGSGSSSGM"
     gene            complement(1874..3625)
                     /locus_tag="VF516_24095"
     CDS             complement(1874..3625)
                     /locus_tag="VF516_24095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX8110844.1"
                     /translation="MRYHVLAADYDETLAHAGRIADTTWSALRRLSASGRKLVLVTGR
                     ELEDVLALIPEPELFARIVAENGAVLYRPESKEIRLLAEPPPPRFVEELRARGVVRPA
                     VGRVIVASREPHQDTVLHVIRDLGLELQVIFNKGAVMVLPTGVNKATGLAAALGELGL
                     SPHNAVGVGDAENDHALLVACECGVAVANALPALRDKADLVTERPGGDGVAELTDRLI
                     ASDLAEVGPGLVRHRIEIGSIEIGSTDHAAITFDPYAANLLVCGTSGSGKSTLTTGLL
                     ERLAEAGYQFAIIDPEGDHVSLDFAVVLGGQQREPLVEEVVDVMRDPSHSVVVNLLGV
                     AASHRPECFERLLAALAEFRVRTGRPHWLVVDEAHHVVPADRQPAADPIAMPSRGMIY
                     VTVHPGSVAPPILGTVDTVLIVGERPRDTLVELCRAAGHPVPAVRATERLAIGHALYW
                     RVGEPDTAVIRIEPPKHERRRHSRKYAEGNLGQARSFYFRGPEAKLNLRAHNLQMFVQ
                     LGEGVDDDTWQYHRRLGDYSRWFREEVKDDQLAREAEAIERSGLTASAGRAAIRAAVE
                     QRYTLPSDAPSGRLDVA"
BASE COUNT          561 a         1402 c         1312 g          603 t
ORIGIN      
        1 atgggccgca ctgtatcgcc gccgcgctcc cggatctgct cgaacacaaa tgcgacaggt
       61 gcgacgtacg tggacgccgc acctgccgct ccggccgatc acgcagcgat cgaccgcacc
      121 gtgtgcgcgg tgatcacatc cccgagctcg agcccgagcc cgagcccggc gctgccgccg
      181 cgagcgtgat cacgccgaac aggccgtgcg cctcgtcctg cggtccggac acgaagtaga
      241 cgccggccgc ctgcacgctc tgcacgccgc tgccggtgcc gatcgcgagg ccccacagcc
      301 cgtcgatctt gagcggggcg ccggccgtgg tcatcagctg atcgatgatc gagccgtcct
      361 tcgggttgac ctcggtgatc gcgccgtcgc cgaagttgcc gaccagcagc acgccgggcg
      421 ccagcgacag gtcgacgccc agcgccatgc cccacggcgc gttgaactgc gcgccgctcg
      481 aggtccacag cacgttgccg cacaggtcga atgcgacgac gaagccgagt cccgcgccgg
      541 cgaccgagtc ggtcttctcg gggttctgct gcgcgtaggt cacgtacacg atgccgttga
      601 acaccgcgac gttgaacggc gcgaagcccg cccggacgct cgccggggtc gagaacatcg
      661 gcgtggtcac gaggttgaat ttctcgtcga acacgtcgat ccgggcgttg tggaagtcgg
      721 ccgcgagtac cagcacgccc ggctgcgggg cgttgcccat gccgccgttg cccatgcccg
      781 tgcccgtgcc ggcgttgcca ctgccggtgc ccgtgcccgc gctgcccatg ccctggccat
      841 tgccctgggt cccgctgccc atgctgccgt cgcacgtccc gctggtggcg gagcacgtgg
      901 cgccggcctt gacgtcgagc accgcgacgc cggtgtagct cgcgccgacc ttcgagcggt
      961 cgacgagcac ctggccgccg gtcgtgttga ggctggtgtt gacgccgatc agcgggcctg
     1021 tctcgctggc gacgatcagg ttggccggtc cgcaggcctg ggcgttcgcg cccttgccat
     1081 ccatctgcat cgcgttcgag ggattgaccg cgatgccggt gatgccctca ccgagatcga
     1141 tcgagctgga cgccacgttc agggtgccct ggccctgccc ttgaccctgg ccctgccctt
     1201 gaccctggcc ggtgccgccc tgctgggcac ccgccgggtg gcccgcggca tcgaggatcg
     1261 acagcttgcc ggtcgcgttg tcggtgatcc agaaccagcc ctggaacccg acgatgcccc
     1321 acgcgttgac cagcgacgat tcgacgctgg ccgccttgcc ggactggtcc gcggtcaggt
     1381 tcgtgaccga gaccagcttg gaggcccccg tgccactgcc gcctgtagcc cctcctcctc
     1441 ccgccagctc cgggctgccc gtgctctcgc cggccatgca gccggcggca agcaatagtg
     1501 caccgatcgc accactccta gcactaaaat ggattgccat gatacaacct cctgcgggaa
     1561 aaccgcgacg gacgtttttg caggtggtgc gccatttccc ggcggaggta ttctcccggc
     1621 tttagcccgt cggtgcgcgg cggaggcgct cgccgcgctg tcagcgatgg gcgctaatcg
     1681 tcactttgac agtcattaca gaatgattat cagcaactct cgtactcccg ggcccagaat
     1741 tcgcccgata taccagacct cacgtacgta tatatccgca caatccaatt cgatctaatc
     1801 aggatgatgg atcggcactc gtccgagtgc aaccccgcgc tcgggcagca tcggattcaa
     1861 cgcattccgt gcgtcacgct acgtcgagcc ggccgctcgg cgcgtcggac ggcagcgtgt
     1921 agcgctgctc gaccgcggcg cggatcgcag cgcggccggc gcttgcggtc aggccgctgc
     1981 gctcgatcgc ctcggcctca cgggcgagct ggtcgtcctt gacctcttcg cggaaccacc
     2041 gcgagtagtc gccgaggcgg cgatggtact gccaggtgtc gtcgtcgacg ccctcgccga
     2101 gctggacgaa catctggagg ttgtgggccc gcaggttgag cttggcctcg ggcccgcgaa
     2161 agtagaagct gcgcgcctgg ccgaggttgc cctcggcgta cttgcgcgag tgccggcggc
     2221 gctcgtgctt gggcggctcg atccggatca ccgcggtgtc gggctcgccg acccgccagt
     2281 acagcgcgtg cccgatcgcg aggcgctccg tggcgcggac cgccggcacc gggtgaccgg
     2341 cggcccggca cagctcgacg agggtgtcgc gcggccgctc gccgacgatc agcaccgtgt
     2401 ccaccgtgcc gaggatcggc ggcgcgacgc tgccggggtg caccgtcacg tagatcatgc
     2461 cgcgcgacgg catcgcgatc ggatccgccg ccggctggcg atcggcgggc accacgtggt
     2521 gggcctcgtc gaccacgagc cagtgcggcc ggccggtgcg gacgcggaac tccgcgagcg
     2581 ccgccagcag gcgctcgaag cactccgggc ggtgcgacgc ggccaccccg agcaggttga
     2641 cgaccacgct gtgcgacgga tcgcgcatca cgtcgaccac ctcctcgacc aggggctcgc
     2701 gctgctggcc gccgagcacc accgcgaagt cgagcgagac atgatcgcct tccggatcga
     2761 tgatcgcgaa ctgatagccc gcttcggcga ggcgctcgag cagaccggtg gtcagggtcg
     2821 acttgccgct gcccgacgtc ccgcacacca ggaggttggc ggcgtacgga tcgaaggtga
     2881 tcgcggcgtg gtcggtcgat ccgatctcga tcgacccgat ctcgatccgg tggcgcacga
     2941 gccccgggcc gacctcggcg aggtcgctcg cgatcagccg atcggtcaac tcggccaccc
     3001 cgtcgccgcc cggccgctcg gtcaccaggt ccgccttgtc gcgcagcgcc ggcagcgcgt
     3061 tggcgaccgc gaccccgcac tcgcaggcga ccagcagcgc gtggtcgttc tcggcgtcgc
     3121 cgactccgac ggcattgtgc ggcgacaggc cgagctcgcc gagcgccgcg gccaggccgg
     3181 tcgccttgtt gacgccggtc ggcagcacca tcaccgcgcc cttgttgaag atcacctgca
     3241 gctcgagccc gagatcgcgg atcacgtgca gcaccgtatc ctgatgaggc tcgcggctcg
     3301 cgacgatcac ccggccgacc gcgggtcgaa cgacgccgcg ggcgcggagc tcctcgacga
     3361 agcgcggcgg cggcggctcc gccagcagcc ggatctcctt gctctccggc cggtacagca
     3421 ccgcgccgtt ctcggcgacg atccgcgcga acagctcggg ctcggggatc agcgccagca
     3481 cgtcctcgag ctcgcggccg gtcaccagca cgagcttgcg gcccgaggcg gacagccggc
     3541 gcagggccga ccacgtcgtg tccgcgatgc gcccggcgtg cgcgagcgtc tcgtcgtagt
     3601 cggcggcaag cacgtgatag cgcatcactc attcaacttt tgcaggaacg gcaccacgcg
     3661 cgtccgagca caccggggcg aagccgcgcc acgctcggtg cggcggcgtc aaggaaactc
     3721 gcagtgcggg tcctgctccc gcggatcggg aacacgtcag tggtcgtagc gcttgcgctt
     3781 gggcgtcaga ccgtactgct tggccttctc gaagaacgtc cggaccggca tcgccaggag
     3841 gcccgcggcg cgggtctggt tgccgccggt cgcctcga
//
LOCUS       DASVOR010001261         5029 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_539012,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001261 DASVOR010000000
VERSION     DASVOR010001261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539012"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VF516_24100"
     CDS             complement(<1..1196)
                     /locus_tag="VF516_24100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8110845.1"
                     /translation="MTALYAVAFLSSLGFSIVVPFMVFLVTRFGGNAFVLGAIGAAFW
                     GAQLVGSIWLGALSDRIGRKRVLFRSQLGAMAAWLIFLCALSAPRVVLGRVEGAAVGT
                     FALTLPLALIALARITDGLFNGSISVANAYLADLADDDERKLGYARLGAASSFGFVVG
                     PVIAGFLARTEIGMTAVLGLALVLSAFAALLVRVRLPAVPPRPTTAIEVVRTGGVRAH
                     KPLGGGCPEAVRHPRWRVRAVLAIPALRPMIALYFLIYLAFSIYVTALPIHAVADVGC
                     TATQLARVYVTLALTLAVTERFVLPRIVRKLATPVIAAAGCSLLVVAYLLISRSSEPA
                     LLGAAVFYGVGNGLMWPSYLVMLSRSGPAESQGSIQGVGSSAGSLASILGTVTGGVLF
                     VAIGVAT"
     gene            complement(1253..3292)
                     /locus_tag="VF516_24105"
     CDS             complement(1253..3292)
                     /locus_tag="VF516_24105"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8110846.1"
                     /translation="MRVAVRWVFGAAKNLRDPHTFHHMSLIAFLAWVGLGADGLSSSA
                     YGPEEAFKALGSWKELVPGGCGVDATVKTCQFVPAFGYLAIFLAIAIAATVFIISWGY
                     SRIIEEFPHGGGGYVVASKHLGPYVGAISGCALLVDYVLTIAISLAAGGDAVFNFLPA
                     AWGVAKLPTVLSVLGILTLMNLRGIKESVTILTPIFLVFLVTHVILIFGAVGANLGNA
                     GQVVNMVGSEGSAHIGVAGLVGVLLVFLRAFSLGGGTFTGIEAVSNGIAIMREPRVRT
                     AKRTMILMATSLAITASGILLAYLLLGVLPQKPEVVGGSAPTMNGLLSARFVGDGPLA
                     TGFIIVTMISEGALLFVAAQAGFVDGPRVMSNMALDQWMPHRMAALSEQLTMRNGVYL
                     MAGAAFVVLLYTGGSVEALVVMYSINVFITFTLSNVAMIRHAFHTKQGKWWRDVFVHG
                     LAASVCGMILVVVVVEKFTEGGWLTTLITSALVVLCFLVRRHYRTVGTKVAALSKELQ
                     LDVPPPDIEPPPEELDPDKPTAVVLAGGYGGLGLHTVLQIPRVFPGQFEQVVFISAGV
                     LDAGVFKGRDEVDALDTKLQADLAKYVAFARTQLGWAADSDHAIGTDAVAELDHLCRE
                     IALRFPRSVFFAGKLIFQKESWYQRILHNETAAAVERRLQFAGLPMIVMPIRMFH"
     gene            complement(3351..4028)
                     /locus_tag="VF516_24110"
     CDS             complement(3351..4028)
                     /locus_tag="VF516_24110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110847.1"
                     /translation="MSAIILVIDDDAQMRRLLRNVLIGHGYTLLEAASVATAIEVVTR
                     HQPDLVLLDLGLPDGDGLEVIRTMRPDDRTPIIVLSARDNEGDKVVALDLGADDYLTK
                     PFGAGELLARIRVALRRAGLAPTDVITVGPIRIDQPRHEVTVDGEVVHLTPIEFKLLL
                     ELARSAGRVLTHRHLLQEVWGPEVVEQNQYLRVHVAALRRKIERDPARPRWLVTEAGI
                     GYRLRDT"
     gene            complement(4179..4316)
                     /locus_tag="VF516_24115"
     CDS             complement(4179..4316)
                     /locus_tag="VF516_24115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110848.1"
                     /translation="MLGWALVLLGGMRIAVALVTGETWAVEVTIAALMMPAGVRLLTR
                     R"
     gene            4842..>5029
                     /locus_tag="VF516_24120"
     CDS             4842..>5029
                     /locus_tag="VF516_24120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110849.1"
                     /translation="MKIMRDGMPAAEAGGVPASLTQSRCRLEVGLISDAVPRGSTMCA
                     RVGAISFALVATSIPVTG"
BASE COUNT          920 a         1724 c         1698 g          687 t
ORIGIN      
        1 gtcgcgacac cgatcgccac gaacaggacg ccgccggtca ccgtgccgag gatgctggcc
       61 aggctcccgg cgctgctgcc cacgccctgg atcgagccct gagattccgc cgggccgctg
      121 cgcgacagca tcacgaggta cgacggccac atcagcccgt tgccgacgcc gtagaacacg
      181 gccgcgccaa gcagcgccgg ctcggagctc cggctgatca gcagataggc gacgaccagc
      241 aacgagcagc cggccgcggc gatcaccggc gtggcgagct tgcgcacgat cctcggcagc
      301 acgaaccgct ccgtgacggc cagcgtgagc gcgagcgtca cgtacacccg cgcgagctgg
      361 gtcgccgtgc atccgacgtc ggccacggcg tggatcggca gcgcggtcac gtagatgctg
      421 aatgcgaggt agatcaggaa gtacagcgcg atcatcggac gcagcgccgg gatcgcgagg
      481 acggcgcgca cgcgccagcg cggatggcgc acggcctcgg ggcagccacc gccgagcggc
      541 ttgtgcgcgc ggacgccgcc ggtgcgcacg acctcgatcg cggtggtggg gcgaggcggt
      601 accgcaggga ggcgaacccg gaccagcagc gcggcgaacg ccgacagcac gagcgcgagc
      661 ccgagcacgg cggtcatgcc gatctccgtg cgcgcgagga agccggcgat caccgggccg
      721 acgacgaacc cgaagctcga cgccgcgccg agccgcgcgt agcccagctt gcgctcgtcg
      781 tcgtcggcga ggtccgccag gtacgcgttg gcaaccgaga tgctgccgtt gaacaggccg
      841 tcggtgatgc gggcgagcgc gatcagggcg agcggcagcg tcagcgcgaa cgtgcccacc
      901 gccgcgccct cgacccggcc gagcacgacg cgcggggcgc tcagcgcgca caggaagatc
      961 agccacgccg ccatcgcgcc gagctggctg cggaacagca cccgcttgcg gccgatccga
     1021 tcggagagcg cgccgagcca gatcgatccg accagctgtg cgccccagaa cgcggcgccg
     1081 atcgcgccga gcacgaaggc gttgccaccg aaccgggtga ccagaaagac catgaacggc
     1141 accacgatgc tgaagccgag gctcgacagg aacgcgacgg cgtagagcgc ggtcatccgt
     1201 cactgtgggc cgggcgccag acgcgcgcga tggacccgac gaccgaggtt ggctagtgaa
     1261 acatccggat cggcatcacg atcatcggaa ggcccgcgaa ctggagccgg cgctcgaccg
     1321 ccgctgcggt ctcgttgtgc aggatccgct ggtaccagct ctccttctgg aagatcagct
     1381 tgccggcgaa gaatacggac cgtggaaagc gcagcgcgat ctcgcggcac aggtggtcga
     1441 gctcggccac cgcgtcggtt ccgatcgcat gatcgctgtc ggcggcccag cccagctgag
     1501 tacgggcgaa cgcgacgtac ttggcgagat ccgcctggag cttggtgtcg agcgcgtcga
     1561 cctcgtcccg gcccttgaac acgccggcat cgaggacgcc cgccgagatg aagacgacct
     1621 gctcgaactg gcccggaaac acccgcggga tctggagcac cgtgtgtagc ccgaggccac
     1681 catagccgcc ggcgagcacg acggcggtcg gcttgtcagg gtccagctcc tctggcgggg
     1741 gctcgatgtc cggcggcgga acatcgagct ggagctcctt cgatagcgcg gcgaccttgg
     1801 tgcccacggt ccgatagtgg cgccggacca ggaagcagag cacgacgagc gccgaggtga
     1861 tcagcgtggt cagccatcca ccttccgtga acttctcgac gacaacaacc acgagaatca
     1921 tgccgcacac gctcgccgcc aggccgtgga cgaacacgtc gcgccaccac ttgccttgct
     1981 tggtatggaa cgcatggcgg atcatcgcca cgttcgacag cgtgaacgtg atgaacacgt
     2041 tgatgctgta catgaccacg agtgcctcga ccgagccgcc ggtatagagg agcacgacga
     2101 acgctgcgcc ggccatcagg tagacgccgt tgcgcatcgt cagctgctcg gacagcgccg
     2161 ccatgcgatg cggcatccac tggtcgagcg ccatgttcga catcacccgc ggcccgtcca
     2221 cgaagccggc ctgcgcggcg acgaacagca gtgcaccctc ggagatcatc gtcacgatga
     2281 tgaacccggt cgcgagcggg ccatcgccga cgaatcgcgc cgacaatagc ccgttcatcg
     2341 ttggcgcgct gccgccgacc acctccggct tctgcggcag gacgccgagc aagagatacg
     2401 cgagcaggat gccgctcgcg gtgatcgcga gcgaggtcgc catcaggatc atcgtgcgct
     2461 tggcggtgcg cacccgcggc tcgcgcatga tcgcgatgcc gttcgacacc gcctcgattc
     2521 cggtgaacgt gccgccgccg agggagaatg cgcgcaggaa cacgagcagg acaccgacga
     2581 gcccggcgac gccgatgtgc gccgaacctt ccgacccgac catgttcacc acctggcccg
     2641 cgttgccgag gttcgctccc acggccccga agatcaggat cacgtgggtg acgaggaaca
     2701 ccaggaagat cggcgtgagg atcgtcaccg actccttgat cccgcgcagg ttcatcagcg
     2761 tcaggatccc gagcacggac agcacggtcg gaagcttcgc gacgccccag gcggcgggca
     2821 ggaagttgaa cacggcgtcg ccgccggccg cgagcgagat cgcgatcgtc agcacgtagt
     2881 cgacgagcag cgcgcagccg gagatcgcgc cgacgtacgg gccgaggtgc ttgctcgcca
     2941 cgacgtaccc gccgccgccg tgcgggaact cctcgatgat gcggctgtag ccccacgaga
     3001 tgatgaacac ggtggcggcg atcgcgattg ccaggaagat cgcgaggtag ccgaacgcgg
     3061 gcacgaactg gcaggtcttg acggtcgcgt cgaccccgca acccccgggc accagctcct
     3121 tccagctacc cagcgccttg aacgcctctt ccggcccgta ggccgacgac gacagcccgt
     3181 ccgcgcccag cccgacccac gccaggaagg cgatcaggct catgtggtgg aacgtgtgtg
     3241 gatcccggag gttcttggcc gcgccgaaga cccagcgcac agcgactcgc acccaggatc
     3301 catagcggtc ccttcataaa gaaggcataa agattgcgtt cagagaccgt tcaggtgtcg
     3361 cgcaagcggt acccgatgcc cgcttcggtg accagccagc ggggccgtgc agggtcgcgc
     3421 tcgatcttgc ggcgcagcgc cgcgacatgg acgcgcaggt actggttctg ctcgacgacc
     3481 tccggccccc acacctcctg gagcaggtgc cgatgcgtga gcacgcgacc tgcggagcgc
     3541 gccagctcga ggagcagctt gaactcgatg ggcgtcaggt gcacgacctc gccatcgacc
     3601 gtcacctcgt gacgaggctg gtcgatccgg atcgggccga cggtgatcac gtcggtcggg
     3661 gccaggccgg cgcggcgcag cgcgacgcgg atgcgcgcga gcagctcgcc cgcaccgaac
     3721 ggcttggtga gatagtcgtc ggcgcccaga tcgagcgcga cgaccttgtc gccctcgttg
     3781 tcgcgcgcgg acagcacgat gatcggcgtc ctgtcatcgg gacgcatcgt ccggatcacc
     3841 tcgagcccat cgccgtcggg caatccgaga tcgagcagca ccaggtcggg ctggtgacgc
     3901 gtgacgacct cgatcgcggt ggcgacgctc gcagcctcga gcagcgtgta accgtgaccg
     3961 atcaggacat tgcgcaggag ccggcgcatc tgcgcgtcat cgtcgatgac caggatgatc
     4021 gcgctcatgc ggcgggatcc tcctggggac ggatgaggct catcggcgac acgagcgttg
     4081 gtagcacgat ccagaacatc gctccgccac cgccgcgagt cagcgcatcg atgcggccac
     4141 cgtgaggctc gatcaaacgg agacgaggtc gagcaagctc accttcgcgt gagcaagcga
     4201 acaccggccg gcatcatcaa cgccgcgatc gtcacctcga cagcccatgt ctcgccggtg
     4261 acgagtgcga ccgcgatgcg catgccgccg aggaggacga gtgcccagcc aagcacgatc
     4321 tcctcgtgcg gtcgcgcgct cgcgctgcgc ggccgctccg gaggagtacg gtaaggaccg
     4381 aacacggtag gctctgatta cctccgaccg cataaagatc gtctcaaggc gccgctaggc
     4441 ttccgtaaat actccaccag aacaacgttg ttccgccgat cggatcgcgg cctcgttctc
     4501 gtcaggaggc tgcacgatcg cgggcgcgca gccatcaagc gcacacctgg cgggctggag
     4561 cgcgcaccgt ggtcaagatc gacggccgcc ctccgtgcgc cggtgctgca ggcagagatc
     4621 cgcgcgatcg tcaggcaagc gctggcgtga cgtgcacccg gccatctgcg ctccgatccg
     4681 ggcggcggtc atgcagcggc gcgcacgagg agcgcgcaag cccgcgtccc gatcgaacac
     4741 gtcgcgcgga tcttgcgcat tcacgacgcc gcctggccgc cgatcgtgcg ctggacggcc
     4801 acccgcaccg acgcacgaca cctggtcgtg atgaaaactt gatgaaaatc atgcgcgacg
     4861 gcatgcccgc tgcagaagcc gggggagtgc ctgcgagctt gacgcaatcc aggtgtcgtc
     4921 ttgaggtggg cttgatctcg gatgcggtac ccaggggctc gaccatgtgt gcccgcgtcg
     4981 gtgccatcag ctttgccctc gtcgcaacgt ccatcccggt caccgggtt
//
LOCUS       DASVOR010001262         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_509567,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001262 DASVOR010000000
VERSION     DASVOR010001262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_509567"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VF516_24125"
     CDS             complement(<1..412)
                     /locus_tag="VF516_24125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110850.1"
                     /translation="MRALDAARRHPGILLLGLALIAAAAIYAPTLGRGLVSYDDPWLV
                     RDNFVVAQPSAHALRAIFFDLDPPVRYALGAEYLPVRDVSVMLDVALWGDAYGGFHLT
                     NLAIYLAAIALGFAMLDGFGVDRTIAGLAVLIWAI"
     gene            517..>1581
                     /locus_tag="VF516_24130"
     CDS             517..>1581
                     /locus_tag="VF516_24130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110851.1"
                     /translation="MALRGQRLWASPSAADLRVLVEAAAVIAATWLLLGWTFQRAITQ
                     ADGTKAGVPLTQGALHAGLDWTRNLYRFGVIGGSEIHGFGGSSPVVQLCAALGLSTTT
                     TVNVVTVFVQLGFGFFSLKAIEALVARARGAAFRLSVAQRITAIWLCSFAPVLGWRLA
                     YGHENLLLGLIPLYTALALLWAARAGTLTATALGFAALVVFNGVSGFGAQTLIYSAVF
                     GAPLVVVTVLDAPRGARWGRPQWIATAALAAGVLVALPRLVPMIHHAFGDDATRSVGD
                     VVTYSLGTASGADWLTSIPWTPRFAAREFATRHEHNFPVGPIVLLVALAWPRRLSRAT
                     PWALVAGAALAIALADDLAPV"
BASE COUNT          192 a          574 c          589 g          226 t
ORIGIN      
        1 ggatcgccca gatcagcacc gcgagcccgg cgatcgtgcg atcgacgccg aacccgtcga
       61 gcatcgcgaa ccccagcgcg atcgccgcca ggtagatcgc gaggttggtg aggtgaaacc
      121 cgccgtacgc gtcgccccac agcgcgacgt cgagcatcac cgacacgtcg cgcaccggca
      181 ggtactcggc gccgagcgcg tagcgcaccg gcggatcgag atcgaagaag atcgcgcgca
      241 gcgcgtgggc cgagggctgc gcgaccacga agttgtcgcg aaccagccac ggatcgtcgt
      301 agctgaccag gccgcggccc agcgtcggcg cgtagatcgc cgccgcggcg atcagcgcga
      361 gcccgagcag caggatcccg ggatgccgcc gagcggcatc gagcgcgcgc atgccggcga
      421 gcgtagcacc tggggccgcg gctccgggcc gcgcctcggc cgcacctcgg tgcagctgga
      481 tccggcgcgg cggcgatgtt acgacctggg tccagcatgg cgttgcgcgg tcagcggctg
      541 tgggcttcgc cgagtgcagc cgacctgcgc gtgctcgtcg aggccgcggc ggtgatcgcg
      601 gcgacctggc tcttgctcgg ctggacgttc cagcgcgcga tcacgcaggc cgatggcacc
      661 aaggcgggcg tgccgctcac ccagggcgcg ctccacgccg ggctcgactg gacccgcaac
      721 ctgtaccggt tcggcgtgat cggcggcagc gagatccacg ggttcggcgg cagctcgccg
      781 gtggtccagc tgtgcgcggc gctcggcctg tcgaccacga cgacggtcaa cgtcgtcacc
      841 gtgttcgtcc agctcgggtt cgggttcttc agcctcaagg cgatcgaggc gctggtcgcg
      901 cgcgcacggg gcgcggcgtt ccggctgtcg gtcgcccagc ggatcacggc gatctggctg
      961 tgcagcttcg cgccggtgct cggctggcgc ctcgcctacg gccacgagaa cctgctgctc
     1021 ggcctgatcc cgctctacac cgcgctcgcg ctgctgtggg ccgcgcgcgc cggcacgctg
     1081 accgccaccg cgctcgggtt cgcggcgctg gtcgtgttca acggcgtgtc cgggttcggc
     1141 gcgcagaccc tgatctacag cgctgtgttc ggggcgccgc tcgtggtcgt cacggtgctc
     1201 gatgcgccgc gcggcgcgcg ctggggccgg ccgcagtgga tcgccaccgc cgcgctcgcc
     1261 gcgggggtgc tggtcgcgtt gccccggctg gtgccgatga tccaccacgc gttcggggac
     1321 gacgccacgc gcagcgtcgg cgacgtggtc acctactcgc tgggcaccgc gagcggcgcc
     1381 gactggctga cctcgatccc gtggacgccg cggttcgcgg cccgagagtt cgcgacccgg
     1441 cacgagcaca acttcccggt cggcccgatc gtcctgctgg tcgcgctggc gtggccgcgc
     1501 aggctgtcgc gcgcgacgcc gtgggcgctc gtcgccggcg cggcgctggc gatcgcgctc
     1561 gccgacgatc tggcgccggt g
//
LOCUS       DASVOR010001263         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_568589,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001263 DASVOR010000000
VERSION     DASVOR010001263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568589"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VF516_24135"
     CDS             complement(<1..618)
                     /locus_tag="VF516_24135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110852.1"
                     /translation="MVSHDRRLVGARRGVMARLLDRVRHQLRRAPSWAWLPVFYALVV
                     GWTYRDLWHQHGVPTGFGWDVVDTHGPDLEFMARELREGRFSLWNPWDKGGYAVYADP
                     IVCRYYPFVWPFVAWGAAFGVSWWLIQLVVLGHHVAMAACMHGFLRSRGLSTRAAMIG
                     GVGLVVSAPVLIHKASNILWPLTWVPLVWLAIDAAVARPSWRRGAA"
     gene            785..3373
                     /locus_tag="VF516_24140"
     CDS             785..3373
                     /locus_tag="VF516_24140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110853.1"
                     /translation="MIQPAVGWLLGPELIANLRSIVRRKGHRGDPRTWMAPNRNKAHD
                     EYTEQRDGRLRPLTGDIVERDLWFDYIADLGDSTNGTYAAAFACQVDLQLDLAQGRSL
                     PTVRGEDLALPCTLHVRGERETDARTDTDPETETTTGTVRGRLPRGEFLFVGGDTAYH
                     VADEVTVRDRVARPFNWATQDLTERRWASDGNRPLLARGKTRIYGIPGNHDWYNDLHG
                     FSLLFLGPREPEPADQAPPRGGKETRSEPIALDGFAPQQQASYVAIQLPYGWQLWGLD
                     IDQGIDTRQTAYFRSLLPGAEGDPRRDPAKLILCTPSPPVVFHTAKPVREHAAAVRDL
                     SLTPAYARQPAGDGSPKDCSLPPGTCRLDLSGDTHHYARYRWPEHGADGTMAPYMAVV
                     SGLGGAFHHPSFTHAGRLPPVVQYPSREQSLEAVGPKLLNWRSLVSGSWIRAFPIVFG
                     LILAFGSINHEGGSAWVLSRVLSAIPGLHVEPCGNAEMLRGSLLMLLTMVVSGLLFTA
                     AVWLFGRAYEHRLALAKDGQPPRRTYREKISAWARTTYLWCTTAAIAGLTALLSFHLC
                     PQTPSATVSLLDMSVTIVLLLGTIGGFLLAWSHADGHLPRRRKLLLGALGVVHGVAQI
                     GTPWIFARVVQFNIIMIPIVWAPFVVWWFTVRDPARPRPTAGALIGIAGLMLALVCHR
                     VLAPSSSTTPIVAGVVITATWLLQLPSRKLFQQSMTRMALMKVVWIVAWFGGIAALLW
                     AAHGTAPFEDSEPHYVAALHNLVQFIAGSIMAAPLCLAYFAWYLAIAGLANAHNNEVG
                     GAARVTQFRQIIRFHLHRGGLTGYAISVENTQGTESQPPACLQGRNLAFQLRDVFTIA
                     PAEPPA"
     gene            3387..>3750
                     /locus_tag="VF516_24145"
     CDS             3387..>3750
                     /locus_tag="VF516_24145"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110854.1"
                     /translation="MVARLVIAGTASGVGKTTVTVGLTRALRARGLRVAVFKCGPDYL
                     DPTYHVRAADVRCENLDGWMMGRDAVAATFASAARGADVALIEGVMGLYDGASPTGDE
                     GSTAEIAKWLAAPVALVCD"
BASE COUNT          551 a         1411 c         1276 g          512 t
ORIGIN      
        1 cgccgcaccg cgccgccacg acggccgcgc caccgccgcg tcgatcgcca gccacaccag
       61 cggcacccag gtcagcggcc acaggatgtt cgacgccttg tggatcagca ccggcgccga
      121 caccacgagc ccgaccccgc cgatcatcgc cgcgcgcgtc gacagcccgc ggctgcgcag
      181 gaagccgtgc atgcacgccg ccatcgcgac gtggtgcccg agcaccacga gctggatcag
      241 ccaccacgac acgccgaacg ccgcgcccca cgccacgaac ggccacacga acgggtagta
      301 gcggcagacg atcggatcgg cgtacaccgc gtagccgccc ttgtcccacg ggttccacag
      361 cgagaaccgg ccctcgcgca gctcgcgcgc catgaactcg aggtccggcc cgtgggtgtc
      421 gaccacgtcc cagccgaacc cggtcggcac gccgtgctgg tgccagaggt cgcggtacgt
      481 ccaccccacg accagcgcgt agaacacggg cagccacgcc cacgacggcg cgcggcggag
      541 ctggtggcgg acgcgatcga gcaggcgcgc catcaccccg cgtcgggctc cgaccagccg
      601 gcgatcatgc gagaccatct tgctagacgc cggcgcccgg cggtagtgtg gttggcaacg
      661 cgggaggtga cgaggcgtga tcaatctatc tgcagaaccg ctacccaccg gttcgtctga
      721 ccgatccgac gggccatccc agccgtacgc cacgcgccgc gagcccgagc ccgccgcgga
      781 tcgcgtgatc cagcccgccg tgggctggtt gctcgggccg gagctgatcg cgaacctgcg
      841 ctcgatcgtc cgccgcaagg gccaccgcgg cgatccgcgg acgtggatgg cgccgaaccg
      901 gaacaaggcg cacgacgagt acaccgagca gcgcgacggg cgccttcgcc cgctcaccgg
      961 cgacatcgtc gagcgggacc tctggttcga ctacatcgcc gacctcggcg acagcacgaa
     1021 cggcacctac gcggccgcgt tcgcctgcca ggtcgatctc cagctcgacc tcgcgcaggg
     1081 ccggtcgctg ccgaccgtcc ggggcgagga cctggccctg ccctgcacgc tgcacgtgcg
     1141 cggcgagcgc gagaccgacg cccggaccga caccgacccc gagaccgaga ccacgaccgg
     1201 caccgtgcgc ggcaggctgc cgcgcggcga gttcctgttc gtcggcggcg acaccgcgta
     1261 ccacgtcgcg gacgaggtca cggtccgcga tcgcgtcgcg cggccgttca actgggcgac
     1321 ccaggacctc acggagcgcc gctgggccag cgacgggaac cggccgctgc tcgcccgcgg
     1381 caagacgcgg atctacggca tccccgggaa ccacgactgg tacaacgacc tgcatgggtt
     1441 ctcgctgctg ttcctcggac cgcgcgagcc cgagcccgcc gaccaggcac cgccgcgcgg
     1501 cgggaaggaa acgcgcagtg agccgatcgc gctcgacggc ttcgcgcccc agcagcaggc
     1561 gtcgtacgtc gcgatccagc tgccgtacgg ctggcagctg tgggggctcg acatcgacca
     1621 gggcatcgac acgcgccaga ccgcgtattt ccgctccctg ctccccggcg ccgagggcga
     1681 cccgcgccgc gatcccgcca agctgatcct gtgcacgccg tcgccccccg tcgtgttcca
     1741 caccgcgaag ccggtccgcg agcacgccgc ggcggtccgc gatctgagcc tgacgccggc
     1801 ctacgcgcgc cagcccgcgg gcgacggctc gcccaaggac tgcagcctgc cgcccggcac
     1861 gtgccggctc gacctgtccg gcgacaccca ccactacgcg cggtaccgct ggcccgagca
     1921 cggagcggac ggcacgatgg cgccgtacat ggcggtggtc tcgggcctgg gcggggcgtt
     1981 ccatcaccca tccttcacgc atgcgggacg ccttccgccg gtggtccagt accccagccg
     2041 cgagcagtcc ctcgaggcgg tcgggccgaa gctgctcaac tggcgcagcc tggtcagcgg
     2101 cagctggatc cgcgcgttcc cgatcgtctt cggcctgatc ctcgcgttcg gctcgatcaa
     2161 tcacgaggga ggctccgcgt gggtgctcag ccgggtactc tcggcgatcc ccgggctgca
     2221 cgtcgaaccc tgcggcaacg ccgagatgct gcgggggtcg ttgctcatgc tcctcacgat
     2281 ggtcgtcagc ggcctcctgt tcacggcggc ggtctggctg ttcgggcgcg cctacgagca
     2341 tcggcttgcc ctcgccaagg acgggcaacc accgcggcgg acgtatcgcg agaagatctc
     2401 ggcctgggcg cgcacgacct atctgtggtg caccacggcg gcgatcgccg ggctcacggc
     2461 gctgctgtcg ttccacctct gcccgcaaac accctccgcc acggtcagcc tgctcgacat
     2521 gagcgtgacg atcgtgctcc tgctgggcac catcggtggg ttcctcctgg cctggtcgca
     2581 tgccgacggc cacctgccgc gccgccgcaa gctcctgctc ggcgcgctgg gcgtggtgca
     2641 cggcgtcgcg cagatcggga cgccctggat ctttgcgcgc gtggtccagt tcaacatcat
     2701 catgatcccg atcgtgtggg caccgttcgt ggtgtggtgg ttcaccgtgc gggatccggc
     2761 gcggcctcgc ccgaccgcgg gcgcgctgat cggcatcgcc ggtctgatgc tcgcgctggt
     2821 ctgccaccgg gtgctcgctc cgagcagcag caccacgccg atcgtcgccg gggtggtcat
     2881 caccgcgacc tggttgttgc agctgccgtc gcggaagctg ttccagcaga gcatgacgcg
     2941 catggccctg atgaaggtcg tatggatcgt cgcgtggttc ggcggcatcg ccgcgctgct
     3001 gtgggccgcg cacggcaccg cgccgttcga ggactcggag ccgcactacg tggcggcgct
     3061 gcacaacctg gtccagttca tcgccggctc gatcatggcg gcgccgctgt gcctggcgta
     3121 cttcgcctgg tacctcgcga tcgccggcct ggcgaacgct cacaacaacg aggtcggagg
     3181 cgcagcgcgc gtcacgcagt tccgccagat catccgcttc cacctccatc gcggcggcct
     3241 gaccggatac gcgatcagcg tcgagaacac gcaaggtacc gagagccagc cgccggcgtg
     3301 cctgcaaggc agaaacctcg cgttccagct gcgggacgtg ttcacgatcg cgcccgccga
     3361 gccgcccgcg tagaatcgga cgacggatgg tggcgcgcct ggtgatcgca ggcacggcga
     3421 gcggcgtcgg caagaccacc gtcaccgtcg ggctgacccg cgcgctgcgc gcccgcggcc
     3481 tgcgcgtcgc cgtgttcaag tgcggccccg actacctcga tccgacctac cacgtgcgcg
     3541 ccgcggacgt gcgctgcgag aacctcgacg gctggatgat gggccgggac gccgtcgccg
     3601 cgacgttcgc gagcgcggcg cgcggcgccg acgtcgcgct gatcgagggc gtcatggggc
     3661 tgtacgacgg cgcctcgccg accggcgacg agggctcgac cgccgagatc gcgaagtggc
     3721 tcgccgcgcc ggtcgcgctg gtgtgcgacg
//
LOCUS       DASVOR010001264         7644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_480634,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001264 DASVOR010000000
VERSION     DASVOR010001264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7644
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480634"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF516_24150"
     CDS             complement(<1..239)
                     /locus_tag="VF516_24150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110855.1"
                     /translation="MLGAAAAAAAALVLGLGLGSVAIADPAGRDASAATRVFTLTPST
                     HGNPEGVAADPRSGAFFVGATGDGTIYRGTLGNPS"
     gene            complement(447..1154)
                     /locus_tag="VF516_24155"
     CDS             complement(447..1154)
                     /locus_tag="VF516_24155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110856.1"
                     /translation="MAFDSGGPPAVRDFIRPAQLSGPMYAFMGWNRTLAEQAAVPTYP
                     CLIDERHLLGDLLGIVNVPTAVWIDEQGAIVRPPESPGATDAFRSMNLQTREVPKERA
                     ADGRLRRQVYVDALRDWIERGAESEHALAPDEVRRRMRGVDRDASLASAQFRLGVWLA
                     RRGEREAGQRALEAAVQLQPESWRFRRQKIVLSDPALTGQLASTPEFWQAVQALGDRY
                     YYPPTEMAGMPPPLPPR"
     gene            complement(1230..1649)
                     /locus_tag="VF516_24160"
     CDS             complement(1230..1649)
                     /locus_tag="VF516_24160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110857.1"
                     /translation="MPQTILYGALETELAGASADRDRLWIPLEELERSTGWTAKPEGL
                     CRDEACVPVPAARKADWFERSRLDFAAFAAHLGHAIARDEDRGVWSFGPAAGSGAASG
                     TGPVMAPDVRLPDLDGALHALSDYRGKKVLLYCWSSW"
     gene            complement(1730..3244)
                     /locus_tag="VF516_24165"
     CDS             complement(1730..3244)
                     /locus_tag="VF516_24165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110858.1"
                     /translation="MQMRHLWTLAALGAAACGGVLGCGGCSSPRGAMTPPPQGDGGGG
                     GPDGNNPDGNNPDGPCAPSRLPPGGLAKGPLRVDPANPRYFTAGNGAVYLTGAHTWGN
                     FKDRAAVDPPPAFDYSAYLDFLVVHHHNFFRLWTWEQPHSSDNVLLYFTPFAWPRVPR
                     TTPASDGKPPFDLDQFDQAYFDRLCTRVLEARDRGIYVSVMLFDGWDLRNGYNAAGGF
                     PMAKNNNVNGVAATPDQFLSLSNKDITARQEAYVRKVIDTVNGLDNVLYEIANETADS
                     TAEIAWQYHMVDYVKSYEKTKPKQHPVGMTSTFPGTDTDLYASNADWISPNAQLASGD
                     GRKVVLNDTDHSYGWVPLQGAGPLGQRQWVWQTFCLGGAPVFMDPYLETWDVRNTPKP
                     PALDPQWSTIRDAIGDTAVYANKLDLERAVPSPSLCSTGYCLAQPGHQYLVYQPGSGT
                     FQLTVTAGTYHLEWYDPLARQASDGGTKSLSPGQQPFTPPGFASDAVLLLSLVP"
     gene            3386..5476
                     /locus_tag="VF516_24170"
     CDS             3386..5476
                     /locus_tag="VF516_24170"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEX8110859.1"
                     /translation="MPWRRLGRWVKRIALAVAGLIVVAAILGFALDATRWRLFLIGKK
                     LRGEVKEVSWGEMLRMIGPSSRYYLRPIVSEGRSVNSAIQNPFTSAADRDEGAKIFRA
                     RCSPCHGQDGVGDRAPPLNRPAYSHGNADWVVYRIVERGIAETGMPPSGMKEQPIWQV
                     IAYLRSRQVETLGAAHPELAAHLQVGVSPADLIAAPSHPDDWLTYSRTLDGWRYSPLT
                     EITADNVGKLRLRWVHQLTTNDPIVQATPLVTAGAMFVSVPPAGVVALDARTGNAIWH
                     YSRDLPLNMSLCCGRVNRGVALLGSTVFFGTLDAHLVALDAATGTPRWETQVADAADG
                     YSITGAPLAIGDLVIAGVSGGEFGVRGFLTAYAAGTGKQVWRFDTIPGPDEPGHDTWK
                     ADSWQTGGGPTWVTGAYDPELDLVYWGVGNPSPIYSGRDRDGDNLYTNSVIALARQTG
                     KLAWHFQFTPHDEHDWDSNQTPILADVALSGGKRRAIGWPNRNGFYYLLDRATGEFLL
                     GKPFVKQTWAKGLDPHGRPILAEGGRPTRKGALVYPGVAGGTNWQSASYHPGLGLIFV
                     PATEGSSIFTSSPPEKHQPGAPFYASGANTEELEVVVKAIDLTTGDTRWEYRPPPTPG
                     FGLTRSGLLTTAGDLVFGACAGQLFALDARTGNELWRVCLGGVTLAAPISVTVDGHQT
                     IAIAAGRAVFLFGL"
     gene            5572..6288
                     /locus_tag="VF516_24175"
     CDS             5572..6288
                     /locus_tag="VF516_24175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110860.1"
                     /translation="MKQLVLVAFLIAAGCATTPVRSASSKGQNGEPDASSGCDAAIDK
                     GIKRLTAGLRDYAPDPQTLESGIRTFDKIQGALVRRCIVDAWPPEVTQCFATMQRLTD
                     IQACQSRLSAEPSSKLATEIAEATRSMDDQGEESKPRALRRRPSKSREGSREALDMMK
                     AYKADLCNCNDHDLNCGKVATDAYQRAMMDWAATRAGTRIDISAKPDPELEKLTTEVA
                     DCARRVMTPAPGPAGSASAP"
     gene            complement(6320..6847)
                     /locus_tag="VF516_24180"
     CDS             complement(6320..6847)
                     /locus_tag="VF516_24180"
                     /inference="COORDINATES: protein motif:HMM:NF044606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HEX8110861.1"
                     /translation="MLFDSWHSMLHVATRAAIAYLVIVAGLRVIGEQALAKMSAYDLI
                     VTITLGSVVAEIPFAQHIAVVDGIAIMITFVLLQEVIRWAQARSKAARRLVAEKPRLV
                     VWEGHVLDDRVQKWRLTVDEIRAAVRRAGLAGISDVQAVVLENDGEWSVVRRRDCGAD
                     RSAFEGLDVPDQTAG"
     gene            complement(7016..>7644)
                     /locus_tag="VF516_24185"
     CDS             complement(7016..>7644)
                     /locus_tag="VF516_24185"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX8110862.1"
                     /translation="SIHAPVAVVFATTGADGAFTLAGVDHGAYDLSAEADDHVRVTRD
                     SVLGGSRNVELVLDAGLAIAGHVVDRDGNPIPRFTLIVQRPSGLVRSVVTTRSLIDPA
                     GRFALRVAPGDYELIASARGFARNAPLAVAAGATDLRIVLGGGAILRGQVIASDDIVA
                     IDGVAVAQLGLDRAFASLRGPPGTTVALTLRRGEQYVQLTVERRVLRT"
BASE COUNT         1171 a         2738 c         2659 g         1076 t
ORIGIN      
        1 atgctcgggt tgccgagcgt gccgcggtag atcgtcccgt cgccggtcgc gccgacgaag
       61 aacgcgccgc tgcgcggatc ggcggccacg ccctccgggt tgccgtgggt cgacggggtc
      121 agcgtgaaca cccgggtggc cgccgaggcg tcccggccgg ccgggtcggc gatcgccacc
      181 gagccgaggc cgaggccgag aaccagggcg gcggcggccg ccgccgccgc gccgagcatg
      241 gcaaagcttc gcgtcaggtg cctattcgat gtcatgaggt actccggatt cgagggtcca
      301 gtcgcagcgc gattcggtcg cgactatagg caatgatccc gatctggcaa tgatcggatc
      361 ccattcccga tctcgaacac gtgtgcccgt gtacacaaca ggcgcgagcc gaaatatcta
      421 tgtcagctgg cccactcgcc ggagcgtcag cgcggcggga gcggcggcgg catcccggcc
      481 atttcggtcg ggggatagta atagcgatca cccagcgcct ggacggcctg ccagaactcc
      541 ggggtcgagg ccagctgacc ggtcagggcc ggatccgaca gcacgatctt ctggcgccgg
      601 aaccgccagc tctcgggctg gagctggacc gcggcctcga gcgcgcgctg cccggcctcg
      661 cgctcgccgc gccgcgcgag ccacacgccg agccggaact gcgccgaggc gagcgacgcg
      721 tcgcggtcga ccccgcgcat ccggcggcgg acctcgtcgg gcgccagcgc gtgctcgctt
      781 tccgcgccgc gctcgatcca gtcgcgcagc gcgtcgacgt agacctggcg ccggagccgg
      841 ccgtcggccg cccgctcctt gggcacctcg cgggtctgca ggttcatcga gcggaacgcg
      901 tcggtcgccc cgggcgactc gggcggccgc acgatcgcgc cctgctcgtc gatccacacc
      961 gcggtcggca cgttgacgat gccgagcaga tcgccgagca ggtggcgctc gtcgatcaga
     1021 cacggatagg tcggcacggc ggcctgctcg gccagcgtcc ggttccagcc catgaaggcg
     1081 tacatcgggc cggacagctg cgccggccgg atgaagtcgc ggaccgccgg cggcccgccg
     1141 ctgtcgaacg ccacggcgac gacgacgaag ccgcggtccg cgtgctgtgc gtagatgccc
     1201 tgccagaccg gcaggtcgaa ccggcaccct caccaggagg accagcagta cagcagcacc
     1261 ttcttgcccc ggtaatcgga cagcgcgtgc agcgcgccgt cgagatcggg gagccggacg
     1321 tcgggtgcca tcacgggccc ggtgccgctc gccgcgccgc tacccgccgc cgggccgaac
     1381 gaccacacgc cgcgatcctc gtcgcgcgcg atcgcgtgcc cgaggtgcgc ggcgaacgcg
     1441 gcgaagtcga gccggcttcg ctcgaaccag tccgccttgc gcgcggcggg caccggcacg
     1501 caggcctcgt cgcggcatag cccctcgggc ttcgccgtcc acccggtcga gcgctccagc
     1561 tcctcgagcg ggatccagag ccgatcgcga tccgccgatg cgccggccag ctcggtctcc
     1621 agcgcgccgt agagaatggt ctgtggcatg cgcatccgta ccgtgggctc gcccggagtt
     1681 caaccacgat cgatcggcac cgcggcggcg atcgcgacgc cgcccgcggt cacggcacga
     1741 gcgacagcag cagcacggca tcgctggcga accccggcgg cgtgaacggc tgctggcccg
     1801 gggagagcga cttggtcccg ccgtcgctcg cctgccgggc cagcgggtcg taccactcga
     1861 ggtgatacgt gcccgcggtc accgtcagct ggaaggttcc agatcccggc tgatagacca
     1921 ggtactggtg accgggctgt gccagacagt atccggtcga gcacagcgac ggcgacggca
     1981 ccgcgcgctc gaggtcgagc ttgttcgcgt acacggcggt gtcgccgatc gcgtcgcgga
     2041 tcgtgctcca ctgcggatcc agggccgggg gcttcggggt gttgcgcacg tcccaggtct
     2101 cgaggtacgg gtccatgaac accggcgcgc cgccgaggca gaacgtctgc cagacccact
     2161 ggcgctggcc gagcggcccg gcgccttgca gcggcaccca gccatacgag tgatcggtgt
     2221 cgttgagcac gaccttgcgc ccgtcgccgg acgcgagctg cgcgttcggc gagatccagt
     2281 cggcgttgct ggcgtagaga tcggtgtccg tgcccgggaa cgtcgacgtc atgccgacgg
     2341 ggtgctgctt cggcttggtc ttctcgtacg acttcacgta gtcgaccatg tggtattgcc
     2401 aggcgatctc cgcggtcgag tcggcggtct cgttggcgat ctcgtagagc acgttgtcga
     2461 ggccgttcac cgtgtcgatc accttgcgga cgtaggcctc ctggcgcgcg gtgatgtcct
     2521 tgttcgacag cgacaggaac tggtccggcg tggccgcgac cccgttgacg ttgttgttct
     2581 tggccatcgg gaagccgccg gcggcgttgt agccgttgcg caggtcccag ccgtcgaaca
     2641 gcatgaccga cacgtagatc ccgcgatcgc gcgcctcgag cacccgcgtg cacagccggt
     2701 cgaagtacgc ctggtcgaac tggtcgagat cgaacggtgg cttgccgtcg gatgccggcg
     2761 tcgtccgggg gacgcgcggc cacgcgaacg gcgtgaagta cagcagcacg ttgtccgagc
     2821 tgtgcggctg ctcccaggtc cacagccgga agaagttgtg gtggtggacg accaggaagt
     2881 cgaggtatgc gctgtagtcg aacgccggcg gtgggtcgac cgcagcgcgg tccttgaagt
     2941 tgccccaggt atgcgcgccg gtgagataca ccgcgccgtt gcccgccgtg aagtagcgcg
     3001 ggttggccgg atcgacgcgc agcggccctt tcgccaggcc gccggggggc agccgcgacg
     3061 gcgcgcacgg cccgtcgggg ttgttgccgt cggggttgtt gccgtcgggg ccgccgccgc
     3121 ctccatctcc ctggggcggc ggcgtcattg ccccacgcgg cgacgagcat ccgccgcacc
     3181 cgagcacccc gccgcacgcg gcggcaccca gcgccgccag ggtccacaga tggcgcatct
     3241 gcatcctcgg catcctcggc gccgagtgta ccgcgagtcc ccggatgcgg cacctccgcc
     3301 ggtgcgccgc gcgcggggcg agatgccgtc gatgtgcaaa ccccgagcag ttgcgatcgc
     3361 ggcgcggcct gggtagcgtg cgctggtgcc atggcgccga ctgggtagat gggtcaagcg
     3421 catcgcgctg gcggttgccg gtttgatcgt cgtcgcggcg atcctcgggt tcgcgctcga
     3481 tgcgacccgc tggcgcctgt tcctgatcgg gaagaagctg cgcggcgagg tcaaggaggt
     3541 gtcgtggggc gagatgctgc ggatgatcgg cccgagcagc cgctactacc tgcgcccgat
     3601 cgtctccgag ggccgcagcg tcaacagcgc gatccagaac ccgttcacct cggccgccga
     3661 ccgcgacgag ggcgccaaga tcttccgcgc gcgctgctcg ccgtgccacg gccaggacgg
     3721 cgtcggtgat cgcgcaccgc cgctcaaccg ccccgcgtac agccacggca acgccgactg
     3781 ggtcgtgtac cggatcgtcg agcgcgggat cgccgagacc ggcatgccgc cgtcgggcat
     3841 gaaggagcag ccgatctggc aggtcatcgc ctacctgcgc tcgcgccagg tcgagaccct
     3901 gggcgccgcg caccccgaac tcgcggccca cctccaggtc ggcgtcagcc ccgccgacct
     3961 gatcgccgcg ccgagccatc ccgacgactg gctgacctac tcgcgcacgc tcgacggctg
     4021 gcgctacagc ccgctcaccg agatcaccgc cgacaacgtc ggcaagctcc ggctgcgctg
     4081 ggtccaccag ctcaccacca acgatccgat cgtccaggcc acgccgctgg tcaccgccgg
     4141 cgcgatgttc gtgtccgtgc ctcccgccgg cgtggtggcg ctcgacgcca ggaccggcaa
     4201 cgcgatctgg cactacagcc gcgatctgcc gctcaacatg tcgctgtgct gcggccgcgt
     4261 caaccgcggc gtcgccctgc tcggcagcac ggtgttcttc ggcacgctcg acgcccacct
     4321 ggtcgcgctc gacgccgcca ccgggacgcc gcgctgggag acccaggtcg ccgacgccgc
     4381 cgacggctac tcgatcaccg gcgccccgct cgcgatcggc gatctcgtca tcgccggcgt
     4441 gtccggcggc gagttcggcg tccgcggctt cctcaccgcc tacgccgccg gcaccggcaa
     4501 gcaggtctgg cgcttcgaca ccatccccgg cccggacgag ccgggccacg acacctggaa
     4561 agccgactcg tggcagaccg gcggcggacc gacctgggtc accggcgcct acgaccccga
     4621 gctcgatctc gtctactggg gcgtcggcaa cccgtctccg atctactccg gccgcgaccg
     4681 cgacggcgac aacctctaca ccaacagcgt gatcgccctc gcgcgccaga ccggcaagct
     4741 cgcctggcac ttccagttca cgccgcacga cgagcacgac tgggactcca accagacccc
     4801 gatcctcgcc gacgtcgcgc tgtccggcgg caagcgccgc gcgatcggct ggcccaatcg
     4861 caacggcttc tactacctgc tcgatcgcgc caccggcgag ttcctgctcg gcaagccgtt
     4921 cgtcaagcag acctgggcca agggcctcga tccgcacggc cgcccgatcc tcgccgaagg
     4981 cggccgcccg acccgcaagg gcgccctggt ctaccccggc gtcgccggcg gcaccaactg
     5041 gcaatccgcg tcgtaccacc ccggcctggg cctgatcttc gtcccggcga ccgagggcag
     5101 ctcgatcttc accagctcgc cgcccgagaa gcaccagccg ggcgcgccgt tctacgccag
     5161 cggcgccaac accgaggagc tcgaggtggt ggtcaaggcg atcgacctga cgaccggcga
     5221 cacgcgctgg gaataccgac caccgcccac cccgggattc ggcctgaccc gcagcggcct
     5281 gctcaccacc gccggtgacc tggtcttcgg cgcctgcgca ggccagctgt tcgcgctcga
     5341 cgccagaacc ggcaacgagc tgtggcgggt ctgcctcggc ggcgtgaccc tcgccgcgcc
     5401 gatctcggtc accgtcgacg gccaccagac gatcgcgatc gccgccggcc gcgccgtgtt
     5461 cctgttcggg ctgtgacggg cccgtgcgat cgcgctacaa gtgaacaaca gcgatggctc
     5521 gtggggagcc atggtgagct ggcaggacga ggtcaatcag gagggtttgt gatgaagcag
     5581 cttgtgcttg tcgcgtttct gatcgcagcc gggtgcgcca cgacgcccgt acgcagtgca
     5641 tcttcaaagg ggcagaacgg tgagcctgac gcgtcctccg gttgtgacgc ggccattgac
     5701 aagggcatca agcggctcac agccggactc cgagactatg cacccgatcc gcaaacgctc
     5761 gagagcggga tccgcacctt cgacaagatc cagggcgcgc tggtccggcg gtgcatcgtc
     5821 gatgcatggc caccggaggt cacgcaatgc ttcgccacca tgcagcgcct cacggacatc
     5881 caggcctgtc agagtaggct gagcgccgag ccgtcgtcga agctggccac ggagattgcc
     5941 gaggcgacga ggagcatgga cgaccagggc gaggagagca agccgcgggc ccttcgccgt
     6001 cgaccctcga aatcgcgtga aggtagccgc gaggcgctcg acatgatgaa ggcctacaag
     6061 gccgatctct gcaactgcaa cgaccatgat ctgaactgcg gcaaggtggc gacggacgcc
     6121 taccagcggg cgatgatgga ctgggcagca accagggcgg ggacgcggat cgacatcagc
     6181 gcgaagccag atccggagtt ggagaagctc accaccgagg tcgccgactg cgccaggcgt
     6241 gtgatgaccc cggctccagg cccggccggc agcgcgagcg ctccgtgaac aaagcacccc
     6301 gggccagccg cgcggcgctc tacccggcgg tctggtcggg cacgtccagg ccctcgaacg
     6361 ccgagcgatc ggcgccgcaa tcccggcggc gtacgacgga ccactcgccg tcgttctcga
     6421 gcacgaccgc ctggacatcg ctgatcccgg cgaggccggc gcggcggacc gcggcgcgga
     6481 tctcgtcgac ggtcaggcgc catttctgca cccgatcgtc gagcacgtgc ccttcccaga
     6541 cgacgaggcg cggcttctcg gccacgagcc tgcgcgctgc cttggatcgc gcctgcgccc
     6601 agcgaatcac ctcctgcagc aggacgaacg tgatcatgat cgcgatgcca tcgacgaccg
     6661 cgatgtgctg cgcgaacggg atctcggcga cgaccgagcc gagcgtgatc gtgacgatca
     6721 ggtcgtaggc gctcatcttc gcgagcgctt gctccccgat cacgcgcagc cccgcgacga
     6781 tcaccagata ggcgatcgcg gcgcgggtcg cgacgtgcag catggagtgc cagctatcga
     6841 acagcatgag aggaatcgtc gcggagatcg cgatgcatcg cgcgcgccag cgtctcgccg
     6901 caagcgaggt atccgggatt ggcgtaggct gaccgcctca cgctgcggtt agaacggcct
     6961 cgagtccgaa gtggtcggag atgaacccgg tctgcggctg tccagggcgc ccgcctcagg
     7021 tgcgaagcac gcggcgctcg accgtgagct ggacgtactg ctcgccgcgc cgcagcgtga
     7081 gggcgacggt ggtcccgggc ggaccgcgca ggctggcgaa cgcgcggtcg agcccgagct
     7141 gggcgaccgc gacgccgtcg atggcgacga tgtcgtcgct cgcgatcacc tggccgcgca
     7201 ggatcgcccc gccgccgagt acgatgcgga gatcggtcgc cccggccgcg accgccagcg
     7261 gcgcattgcg ggcgaagccg cgcgccgacg cgatcagctc gtagtcaccc ggcgcgaccc
     7321 gcagcgcgaa ccgacccgcc ggatcgatca gcgaccgggt ggtgacgacc gaccgcacga
     7381 gaccgctggg ccgctgcacg atcagggtga accgcgggat cgggttgccg tcgcggtcga
     7441 cgacgtggcc ggcgatcgcg aggccggcat cgagcacgag ctcgacgttg cggctgccgc
     7501 cgagcacgct gtcgcgggtg acccggacgt gatcgtcggc ctcggcggac aggtcgtagg
     7561 cgccgtgatc gacgccggcg agcgtgaacg cgccgtcggc gccggtggtc gcgaacacga
     7621 ccgcgaccgg cgcgtggatc gagg
//
LOCUS       DASVOR010001265         3763 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_129675,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001265 DASVOR010000000
VERSION     DASVOR010001265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129675"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="VF516_24190"
     CDS             complement(<1..1294)
                     /locus_tag="VF516_24190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110863.1"
                     /translation="MFWSKIWLFLLALAAAAALTLALVLPRPAQRAQARGEINRLQTA
                     CSVIHILLADDARNRIDLAGAFARSPEIMNALESASSVPQLDETRMKQVRDVGETVMK
                     AIQGSRKPDFAMLIERRGRVVARVHLDDGDWGDVAAGRPLIDDALAGYLRDDVWVQTG
                     SLFFVSAAPVVKQGAYVGAIVLGHKVTNQLALTLVKSLDVDMGFHLGADGVAGSRTIA
                     FDHGPLQAAIGKLGDDVTTDCQQVRPSDVRAGADDYTAIAARLPGEATARQAYYSVMI
                     RRPEGLGLFGKLRAINDSDLSLASFPWILVGGAFLVVLGLGIGLMLLEVDRPLRRLAA
                     DAVRLAKGDTDRLTEDVHGGKFGSIARSVNIHIDKLGREARSARKDLDQLLGPAPEGG
                     LGTIDLLATSLPTVRPGGPAPAVPPPPSDFRFTDSGSQP"
     gene            complement(1300..2019)
                     /locus_tag="VF516_24195"
     CDS             complement(1300..2019)
                     /locus_tag="VF516_24195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110864.1"
                     /translation="MRLGFLVGILSSIAALAGPVRAGTVVGKLELPPPLERGPVIARG
                     FVDRIENPLADIKKPSLAPYLVVTLESEDRSDAAPAQVNWDVVGESFVRPVVAVPVGA
                     EVVIRNQTKIARNLAAAEDARLLAGPLNPTATKSFRATQPAIYTIGDRDAPHLRGKLV
                     VVATPHVAAIDDSGRFEMTDVPDGSYKLRVFYYDPVGEARGKPSGWLFTTDVTVAPKG
                     RSTRTEVNAKLPPLTAAAGKK"
     gene            complement(2228..2920)
                     /locus_tag="VF516_24200"
     CDS             complement(2228..2920)
                     /locus_tag="VF516_24200"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8110865.1"
                     /translation="MIRQEIKMLKKMRNTATETRSPGRRNVILFLAANPHDSSPLKLG
                     DECAEIQRELRLAPHGDDFSFESRWAVGVDDFMRALTELDPTVIHVSGHGGLGARLML
                     QDAHGLAQPVVARALAMMVETAARSTRVVVLNACYSAELADALRSRVDCVVGMEGAIG
                     DAAARAFATRFYGALGNRRSIGNAVAQGKAALVAKQLPDEQRPRCVTRDGVDARRLVL
                     QTPARLARRRAS"
     gene            complement(2950..3192)
                     /locus_tag="VF516_24205"
     CDS             complement(2950..3192)
                     /locus_tag="VF516_24205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110866.1"
                     /translation="MLDLAAWSEGNLSPHGMHRLASHVVRCRTCQLVVAAVVRDAHAV
                     DSTMQRPCEAERLFEAWTAEHEGIQSCVAALDTCDR"
BASE COUNT          542 a         1296 c         1381 g          544 t
ORIGIN      
        1 acggctgcga gccggagtcg gtgaaccgga aatcggaggg cggcggcggg acggcggggg
       61 ccgggccgcc ggggcggacg gtgggcagcg aggtcgcgag cagatcgatc gtgccgagac
      121 cgccttcggg ggccgggccg agcagctgat cgagatcctt cctggccgat cgggcctcgc
      181 ggccgagctt gtcgatgtgg atgttgacgc tgcgcgcgat cgagccgaac ttgccgccgt
      241 ggacgtcctc ggtgagccgg tcggtgtcgc ccttggcgag ccggacggcg tcggcggcga
      301 gccggcgcag cgggcggtcg acctcgagca gcatcaggcc gatgccgagg ccgagcacga
      361 cgaggaaggc gccgccgacg aggatccacg ggaagctggc gagcgacagg tcgctgtcgt
      421 tgatcgcgcg gagcttgccg aacaggccga ggccctcggg gcgccggatc atcaccgagt
      481 agtaggcctg gcgggcggtg gcctcgccgg gcagccgggc ggcgatcgcg gtgtagtcgt
      541 cggcgccggc gcggacgtcg ctcggccgga cctgctggca gtcggtggtg acgtcgtcgc
      601 cgagcttgcc gatcgcggcc tgcagcgggc cgtggtcgaa cgcgatcgtc cggctgccgg
      661 cgacgccgtc ggcgccgagg tggaacccca tgtcgacgtc gagcgacttg accagggtca
      721 gggcgagctg gttggtgacc ttgtggccga gcacgatggc gccgacgtag gcgccctgct
      781 tgaccaccgg ggcggccgac acgaagaaca gcgagccggt ctgcacccac acgtcgtcgc
      841 gcaggtagcc ggcgagcgcg tcgtcgatca gcgggcggcc ggcggcgacg tcgccccagt
      901 cgccgtcgtc gaggtggacg cgcgccacca cgcggccgcg gcgctcgatc agcatggcga
      961 agtccggctt gcggctgccc tggatcgcct tcatcacggt ctcgccgacg tcgcgcacct
     1021 gcttcatgcg ggtctcgtcg agctggggca cgctcgacgc cgactccagc gcgttcatga
     1081 tctcgggcga gcgcgcgaac gccccggcga ggtcgatccg gttgcgcgcg tcgtcggcga
     1141 gcaggatgtg gatcacgctg cacgcggtct gcagccggtt gatctcgccg cgggcctggg
     1201 cgcgctgggc cggccgcggc agcaccaggg cgagggtgag cgccgcggcg gcggcgaggg
     1261 cgagcaggaa cagccagatc ttggaccaga acaccgacgt cacttctttc ccgccgccgc
     1321 ggtcagcggc ggcagcttcg cgttgacctc ggtccgggtc gagcggccct tcggcgcgac
     1381 cgtcacgtcg gtggtgaaca gccagcccga cggcttgccg cgcgcctcgc cgaccgggtc
     1441 gtaatagaac acgcggagct tgtagctgcc gtcggggacg tcggtcatct cgaaccggcc
     1501 gctgtcgtcg atcgcggcga cgtgcggggt cgcgaccacg acgagcttgc cgcgcaggtg
     1561 gggggcgtcc cggtcgccga tcgtgtagat cgccggctgg gtcgcgcgga acgacttggt
     1621 cgcggtcggg ttgagcgggc cggcgagcag cctggcgtcc tcggcggcgg cgaggttgcg
     1681 cgcgatcttc gtctggttcc tgatgacgac ctcggcgccg accggcacgg cgacgaccgg
     1741 gcgaacgaac gactcgccga cgacgtccca gttgacctgg gccggcgcgg cgtccgaccg
     1801 gtcctcgctc tcgagcgtca cgaccaggta cggcgcgagg ctgggcttct tgatgtcggc
     1861 gagcgggttc tcgatccggt cgacgaagcc ccgggcgatc accgggccgc gctcgagggg
     1921 cggcggcagc tcgagcttgc cgaccacggt gccggcccgg accggccctg cgagggccgc
     1981 gatgctgctg aggattccga ccagaaagcc gaggcgcatg ggcctgccga tcatcgtaaa
     2041 gagctgcact cccggcggca agcggcactg gctcgaaccg gctcggggca gggccttcgc
     2101 gggaaggccc ggaactgcct cgggaagatg acatcggcgg tgcactcccg cgcgggccgg
     2161 cggaccgccc ggtcccgccc gaggcgccgg tcggtccaga acgaccgtcc ctcgacgcgt
     2221 cgggcactca cgaggcgcgc cggcgagcga gcctcgcagg cgtctggagc acgagtcgac
     2281 gcgcatcgac cccgtcccgg gtcacgcacc gcggccgctg ctcgtcgggc agttgcttcg
     2341 cgaccagcgc cgccttgccc tgcgccacgg cgttgccgat cgatcggcgg ttgccgagcg
     2401 cgccgtagaa ccgcgtggcg aacgcccgcg ccgcggcatc accgatcgcg ccctccatcc
     2461 ccacgacgca gtcgacccgg gaccgcagcg cgtcggccag ctccgcgctg tagcacgcgt
     2521 tgagcacgac gacccgggtg ctccgcgcgg cggtctcgac catcatcgcg agggcgcgcg
     2581 ccaccaccgg ttgcgcgagc ccgtgtgcgt cctgcagcat cagcctcgca cccaggccgc
     2641 cgtggccgct gacatggatc acggtggggt cgagctcggt cagggcgcgc atgaagtcgt
     2701 cgacgccgac cgcccagcgc gattcgaagc tgaagtcgtc gccgtggggg gccagcctca
     2761 gctcgcgctg gatctcggcg cattcatcac ccagcttgag cggactgctg tcatgaggat
     2821 ttgcggcaag aaacaagatg acattccgcc ggccaggcga gcgtgtctcc gttgctgtgt
     2881 ttctcatctt cttgagcatc ttgatctcct gtctgatcaa gacgacaagc tgagattcgc
     2941 tccatgcaat cagcgatcac atgtatccag tgcagcaaca caggactgaa tcccctcgtg
     3001 ttccgcagtc cacgcttcga acaggcgctc cgcctcacag gggcgctgca tggtggagtc
     3061 caccgcgtgc gcatcccgga ccaccgcggc gaccaccagc tggcaggtcc ggcaccggac
     3121 cacgtgactc gcgagccggt gcatcccgtg tggtgacagg ttgccttcgc tccaggccgc
     3181 gagatccagc acggcggagg gatccgtaca gctcccgggc atctccgcaa cgaccggtcc
     3241 ggtaccagag gtgatggctc ggaaacccac tcgcggcatc aatccttctc cttcgtcggt
     3301 ggagacgcat gtgtgcagtc gctgcgaacg atcgctggaa ggccacacgg ccctcggtgg
     3361 agtaagacgg cagtttgcgc ggcggacgat gaactttcgg tattcggggc cgcgaaaaaa
     3421 cgcaagtgcc tgaaatcgcg aggccagcgc tggccgacct gcgccacgag tacgcgcgtc
     3481 acccagaacg tcacagggac cgccgtgttg ccggagggga gcggcaagcg ccgtcgctag
     3541 cggctgccca ccgcctgcgg ccgtacgcgg ccgccgctgc accccggcga tcggcgcgca
     3601 acttccggcg cgacccgccg ataacgttgc atgttccgtc caaggctcgc tctgctcccc
     3661 ggtcccctcg cggtggcgat cgcggcggcg gcggggatgg tcgccgcacc gtggctcgac
     3721 gcgctcggcg tgccgaccgg gccgcgctgg ctggtcgcgg ccg
//
LOCUS       DASVOR010001266         3517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_451160,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001266 DASVOR010000000
VERSION     DASVOR010001266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451160"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1728
                     /locus_tag="VF516_24210"
     CDS             <1..1728
                     /locus_tag="VF516_24210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110867.1"
                     /translation="PAPVPRPDPAPVLQLDPAPVPRPDPAPVLRPDPAPRFHLSSLLS
                     WGDLTSAAISPDGSQIAIVTGDALVLHGIGPGRRDRVVIDHGIFSLPPLHWSPDGKRI
                     LAGVTPDPAGQLQAAVVDVDHGTWFRVPVKGTATFLSNEELAVAAFRDRSITFVPAGE
                     HVHATATCAVNGDYTLLQNLAGMADGTTIVETLKGDTHALVVLRRDCRVQGTFSAEPI
                     SSFAVSDTGKVVALAGGDGGRELLEVSLDGKILSRRSVSGDIDQVLGRRGDVDYLATR
                     VQKTHLDRVDLGGSTHGLPSVEGNASFSLAPDGDTLAWVELSGHTRERGRLWRASLRS
                     GLEDRQVLRNNALTVGWSPDGERLAVLVDDGRGPAVVVIDRDRSERRLPLRHLAREAA
                     PVWRDDQRILAQSDDCLTYQWFDLTTDAYGDIMDSEHGGTYWLAGSPRDGMLAVWRNG
                     PPGATNARTEHLWLQAPGGEPVPLHVDDAVRDHLSPSWSPWGELFVRALGTGVVSRVA
                     LGTGELTPVARLPKMPLHPRFDTRLMFAADGNLLAPHVELGMDIWETVPDEQLELPLP
                     VESSVNLVR"
     gene            complement(1750..2424)
                     /locus_tag="VF516_24215"
     CDS             complement(1750..2424)
                     /locus_tag="VF516_24215"
                     /inference="COORDINATES: protein motif:HMM:NF018745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HEX8110868.1"
                     /translation="MKHGRAFHPLDPRSARARLILAIVTGGCAWLAVPWRIAEITRAL
                     LTWDAAGLVLVGVAGLIILRSDARETRRRAAAYDPGRRVVWILVLIASTISLLAATLV
                     VREGRGDTRVLHVALCVATVVISWLVTHSAFTLRYAHLYYRDADRAARIELPGGIELP
                     GGEPPDDLDFAYFAFTIAMCFQVSDATVSGRQIRRTVLGQALLAFLYNTVILALALNL
                     IVGQLG"
     gene            complement(2526..>3517)
                     /locus_tag="VF516_24220"
     CDS             complement(2526..>3517)
                     /locus_tag="VF516_24220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110869.1"
                     /translation="DRFAARDAFVRSATAANSTAIGRSELSGDARTIGYTKMAFDQIL
                     WTQNETRRLPNPRAQFPTAAEASQIARGRQLFSTEVSAGGAGCSSCHHNGDQTVNGVP
                     NDTFQDYNIHEPGVISETTVDGDGPFQRLAADYFFIQGGQPQDEGSRQNISSRNTRHL
                     RSFWDGVPRYLHHGFAHTVREVLLPPDSPLLLPGERGFNFRTVRTDASRRVASSFLGG
                     PTITLPTEVPITVADSSGALGGDGKGPILVSLDAPTQIAPPSTAYPDGRLQVDQLGTT
                     NLAPVIVVTNGVRQINPALATSHVQVIKDTHGKTSQLSQADLDALILYVTSLL"
BASE COUNT          502 a         1207 c         1285 g          523 t
ORIGIN      
        1 cccgcgccgg tcccccgacc cgaccccgcg ccggtcctcc aactcgaccc cgcgccggtc
       61 ccccgccccg accccgcgcc ggtcctccga cccgaccctg cgccgcgctt ccacctctcc
      121 tcgctgctct cgtggggcga cctgacgtcg gcggcgatct cgcccgatgg tagccagatc
      181 gcgatcgtca cgggggacgc gctcgtcctc cacggcatcg gccccgggcg tcgggaccgg
      241 gtcgtcatcg atcacggtat cttttccttg ccaccactgc actggtcccc cgatggcaag
      301 cgcatcctcg ccggcgtcac gccggacccc gccgggcaac tccaggccgc cgtggtcgac
      361 gtcgaccacg gcacctggtt cagggtcccg gtcaagggga cggcaacgtt cctgtcgaac
      421 gaggaacttg ccgtggccgc gttccgcgac cggtcgatca cgttcgtccc cgcgggcgag
      481 cacgtccacg cgaccgcaac ctgtgcggtg aatggcgact acacgctgct gcagaacctc
      541 gccggcatgg cggatggcac gacgatcgtc gagaccctga agggtgacac gcatgccctg
      601 gtggttctcc gtcgagactg ccgggtccaa ggcacgtttt ctgccgagcc gatctcgagc
      661 ttcgcggtga gcgacaccgg gaaggtcgtc gccctcgcgg gcggcgatgg aggcagggag
      721 ctcctcgagg tctccctcga cggcaagatc ctctcgcggc gcagcgtcag cggggacatc
      781 gaccaggtgc tcggtcgccg cggcgacgtt gactacctgg ccacccgcgt gcagaagacg
      841 catctcgacc gcgttgacct gggaggatcg acgcacgggt tgccctccgt ggaaggcaac
      901 gcctcgttca gcctggcgcc ggacggcgac acgctggcct gggtcgagct atccggccac
      961 acccgggaac gaggtcgact gtggcgggcg agcttgcgca gcgggttgga ggaccggcag
     1021 gtgctgcgca acaacgcctt gacggtgggg tggtcacccg atggggaacg cctcgccgtc
     1081 ctggtcgacg atggccgggg cccggcggtg gtcgtcatcg accgggaccg cagcgagcgg
     1141 cgcttgccgc tgcgtcacct cgcgcgcgaa gccgcgccgg tctggcgcga tgatcaacgg
     1201 atcctggcgc agtccgacga ttgcctcacc taccagtggt tcgacctcac taccgacgcg
     1261 tacggggaca tcatggacag cgagcacggg ggcacctact ggctggcggg gtcgccgcgc
     1321 gacgggatgc tcgcggtgtg gcgaaacggg cctcctgggg ccacgaacgc ccgcaccgag
     1381 catctctggc tacaggcgcc aggcggtgag cccgttccgc tccatgtcga tgacgcggta
     1441 agagatcacc tgtcgccgag ctggtcgccg tggggcgagc tgttcgtgcg agcgctcggg
     1501 accggcgtgg tgtcgcgcgt cgcactcggc accggcgagc tcaccccggt cgcgcggttg
     1561 ccgaagatgc cacttcaccc gcggttcgac acccgactca tgttcgcggc cgatggcaac
     1621 ctcctcgcgc cccacgtgga gctcggcatg gacatctggg agaccgtccc cgacgaacag
     1681 ctcgagttgc ctcttccggt cgagtccagc gtcaacctgg tgcgctgacg catcgcatgg
     1741 cggccgcggt cagccgagct ggccgacgat caggttgagc gcgagcgcga ggatgacggt
     1801 gttgtacagg aacgccagca gcgcctggcc cagcacggtg cgccggatct gccggccgga
     1861 gaccgtggca tcggagacct ggaagcacat cgcgatggtg aacgcgaagt acgcgaagtc
     1921 gagatcgtcg ggcggctcgc ctcccggcag ctcgatgccg ccgggcagct cgatgcgggc
     1981 ggcgcgatcg gcgtcgcgat agtagaggtg tgcgtagcgc agggtgaacg cggagtgagt
     2041 gaccagccac gagatcacca cggtcgccac gcacagcgcg acgtgcagca cgcgggtgtc
     2101 gccgcggccc tcgcgcacca cgagggtcgc cgcgagcagg ctgatcgtgc tggcgatcag
     2161 cacgaggatc cacaccaccc ggcgccccgg atcgtaggcg gctgcgcggc ggcgggtctc
     2221 gcgggcgtcc gagcgcagga tgatcaagcc cgcgaccccg accagcacga gtcccgcagc
     2281 gtcccacgtc aggagtgcgc gtgtgatctc ggcgatccgc catggtacgg cgagccaggc
     2341 gcagccgccc gtcacgatcg ccaggatcaa gcgggcccgc gccgacctgg ggtcgagcgg
     2401 atggaacgcg cggccgtgct tcatgctgcg agcgtgcagc accgccggcc gcgggccaag
     2461 tcagcgacgc acggggttca catccaccgg gcgtggcggt gccgcgcacg gcgccgggcg
     2521 cggcgctaca gcagcgacgt gacgtacagg atcagcgcgt cgaggtcggc ctgcgagagc
     2581 tgcgacgtct tgccgtgggt gtccttgatc acctggacgt ggctggtcgc cagcgccggg
     2641 ttgatctggc gcacgccgtt ggtgacgacg atgaccggcg cgaggttggt ggtgccgagc
     2701 tgatcgacct ggagccggcc gtcggggtag gccgtgctgg gcggcgcgat ctgggtcggc
     2761 gcatcgaggc tgaccaggat cgggcccttg ccgtcgccgc cgagcgcgcc gctcgagtcg
     2821 gcgaccgtga tcggcacctc ggtcggcagg gtgatcgtcg ggccgccgag gaagctggag
     2881 gcgacccggc ggctcgcgtc ggtgcgcacg gtgcggaagt tgaagccgcg ctcgccgggg
     2941 agcagcagcg gcgagtcggg cggcagcagg acctcgcgca cggtgtgggc gaagccgtgg
     3001 tgcaggtagc gcggcacgcc atcccagaag ctgcgcaggt gacgggtgtt gcggctcgag
     3061 atgttctgcc ggctgccctc gtcctggggc tggccgccct ggatgaagaa gtagtcggcg
     3121 gccagccgct ggaacggccc gtcgccgtcg accgtggtct cgctgatcac gccgggctcg
     3181 tggatgttgt agtcctggaa cgtgtcgttg ggcacgccgt tgaccgtctg atcgccgttg
     3241 tggtggcagc tcgagcagcc ggcgccgccc gcgctgacct cggtgctgaa cagctgccgg
     3301 ccgcgcgcga tctggctggc ctcggcggcg gtcgggaact gggcgcgcgg gttgggcagc
     3361 cggcgggtct cgttctgcgt ccacaggatc tgatcgaacg ccatcttggt gtacccgatc
     3421 gtccgcgcgt cgccgctgag ctcggagcgg ccgatcgccg tcgagttggc cgcggtcgcg
     3481 ctgcgcacga acgcgtcccg cgcggcgaac cgatcgg
//
LOCUS       DASVOR010001267         2776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363573,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001267 DASVOR010000000
VERSION     DASVOR010001267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363573"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VF516_24225"
     CDS             <1..1140
                     /locus_tag="VF516_24225"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HEX8110870.1"
                     /translation="RATVPAEPAPVANVRAGEWDDNANYRDYVRWLQETPRGIARLDV
                     SDRQFLVVEDAAGKPVPNCKIAITGGQRTVSLVSTASGRALLFPHAMGLGGGVLGAIA
                     TCDQAAAQGTIHADRVDGVTELRLAIERVLPARRTVDLAFVLDTTGSMGEEIEAVKST
                     IRTVAARLGSDQTDVRIGLVEYKDRGDAEVTHAYPFSSDLNAFSRSVARITAGGGGDY
                     PEDMQAGLSLALDQLQWRPDATVRMVVVIADAPPHLDYRDERDYADAARRAAARGIKM
                     FTVSASGMDVTGQIVMRQMAQFTGASNLFVLRGGAGPQSVGGGDPQSSCGGTQGQYAS
                     GNLDQLIVGKVKRELAALDGDPLRIAGRGQDENAKPCDQRIATAN"
     gene            1257..2453
                     /locus_tag="VF516_24230"
     CDS             1257..2453
                     /locus_tag="VF516_24230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8110871.1"
                     /translation="MKTASLLAGVAAIAGACGKDEGAHPGAAPPPIEVGVVEIRPQRV
                     LLRTELPGRTSAIRVAEVRARVDGIVLKRLYREGSEVRGGQPLFQIDPAPFEAALQRA
                     RAQLTSAQASAATARLLAQRYGPLIKTNAISRQDYDNAVAQARSAAASVDAARADVRT
                     AQINLGYTRVYAPISGRSGRSNVTEGAYVQAAQATLLTTVTQLDPIYVDTTWSTVDLM
                     RVRRELERGQLVTVEGKPRVTVMFEDGSAYARPGTLLFTEVNVEPSTGSVPMRALVPN
                     PRGDLLAGMYVRARIDEGSQPDALLVPQRAVTRDRTGRATALVVDHAGKVQLRLLQVD
                     RAIGDAWLVIQGVAPGDRVIVEGLQHVKPGVTVRAVPAPAGLEASAPPAGAQPPPHGG
                     GSPGGS"
     gene            2453..>2776
                     /locus_tag="VF516_24235"
     CDS             2453..>2776
                     /locus_tag="VF516_24235"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8110872.1"
                     /translation="MARFFIDRPIVAWVFALIVMLAGVVSIERMPIAQYPPIAAPAIT
                     ATSTYPGASAETVDTTVTQVIEQQMRGLDNLRYMLSNSDSTGQSQIELTFEPGTDPNT
                     ALIQIQ"
BASE COUNT          401 a          962 c         1062 g          351 t
ORIGIN      
        1 cgcgcgacgg tgccggccga gcccgcgccg gtggcgaacg tccgcgccgg tgaatgggac
       61 gacaacgcga actaccgcga ctacgtccgc tggctgcagg agaccccgcg cgggatcgcg
      121 cggctcgacg tctcggaccg tcagttcctg gtcgtcgagg acgcggcggg caagccggtg
      181 cccaactgca agatcgcgat caccggcggc cagcgcacgg tgtcgctggt cagcacggcg
      241 tcgggccggg cgctgctgtt cccgcacgcg atggggctcg gcggcggcgt gctcggagcg
      301 atcgcgacct gcgaccaggc cgcggcgcag gggacgatcc acgccgatcg cgtcgacggg
      361 gtcaccgagc tccggctcgc gatcgagcgc gtgctgccgg cgcgccgcac cgtcgacctg
      421 gcgttcgtgc tcgataccac gggctcgatg ggcgaggaga tcgaggccgt gaagtcgacg
      481 atccgcacgg tcgccgcgag gctcggctcc gaccagaccg atgtccggat cggcctggtc
      541 gagtacaagg accgcggcga cgccgaggtg acccacgcgt acccgttctc gtcggacctg
      601 aacgcgttct cgcgcagcgt cgcccggatc acggccggcg gcggcggcga ttacccggag
      661 gacatgcagg ccgggctgtc gctcgcgctc gaccagctgc agtggcggcc cgacgcgacc
      721 gtgcggatgg tcgtcgtgat cgccgacgcg ccgccgcacc tcgactaccg ggacgagcgc
      781 gactacgcgg atgccgcgcg gcgcgccgcc gcccgcggga tcaagatgtt cacggtctcg
      841 gcgtcgggca tggacgtcac cggccagatc gtgatgcggc agatggcgca gttcaccggc
      901 gcgagcaacc tgttcgtgct gcgcggcggc gcgggcccgc agtcggtcgg cggcggcgat
      961 ccgcagtcga gctgcggcgg cacccagggg cagtacgcga gcggcaacct cgaccagctg
     1021 atcgtcggga aggtcaagcg cgagctcgcg gcgctcgacg gcgatccgct gcggatcgcg
     1081 ggccgcggcc aggacgagaa cgccaagccg tgtgaccagc gcatcgcgac cgcgaactga
     1141 cccagcgccg gtcgcgcacc ggggcgcacg gccgccgctg caggacgcac ggcgcctgtg
     1201 cgctcgcggc ccgctgcgat cggcggcgcg gtacctgcag ctgcgtcgtc ctggtgatga
     1261 agacggcttc cctgctggcg ggtgtcgcgg cgatcgccgg ggcctgcggc aaggacgagg
     1321 gggcgcatcc gggcgcagcg ccgccgccga tcgaggtcgg ggtcgtcgag atccggccgc
     1381 agcgcgtgct gctgaggacg gagctgccgg ggcgcacgtc ggcgatccgg gtcgccgagg
     1441 tgcgggcgcg cgtcgatggc atcgtgctca agcggctgta ccgcgagggc agcgaggtcc
     1501 ggggcgggca accgctgttc cagatcgatc cggcgccgtt cgaagctgcg ctgcagcggg
     1561 cgcgagccca gctcacgagc gcccaggcat cggcggccac ggcgcggctg ctcgcccagc
     1621 gctacgggcc gctgatcaag accaacgcga tcagccggca ggactacgat aacgcggtcg
     1681 cgcaggccag gagcgcggcc gcgagtgtcg acgcggcgcg cgccgacgtc aggaccgcac
     1741 agatcaacct cgggtacacg cgggtgtacg cgccgatctc ggggcggagc gggcgcagca
     1801 acgtcaccga gggggcctac gtgcaggcgg cccaggcgac gctgctcacg accgtgaccc
     1861 agctcgatcc gatctacgtc gacaccacct ggtcgaccgt ggacctcatg cgcgtgcggc
     1921 gcgagctgga gcgcgggcag ctggtgacgg tcgagggcaa gccgagggtg acggtgatgt
     1981 tcgaggacgg cagcgcgtac gcgcggccgg gcacgctgct gttcacggag gtgaatgtcg
     2041 agccctcgac cggctcggtc ccgatgcgcg cgctggtgcc gaacccgcgc ggcgatctgc
     2101 tggcggggat gtacgtccgc gcgcggatcg acgaaggcag ccagccggac gcgctgctcg
     2161 tgccgcagcg cgcggtcacg cgcgatcgca ccggccgggc gacggcgctg gtcgtcgacc
     2221 acgccggcaa ggtgcagctg cgcctgctgc aggtcgatcg cgcgatcggc gatgcctggt
     2281 tggtgatcca gggtgtcgcg ccgggcgatc gagtgatcgt cgaggggctg cagcacgtga
     2341 agccgggcgt cacggtccgg gccgtgcctg cgccggcggg gctggaagcc tcggcaccgc
     2401 cggccggcgc gcagccgcca ccgcacggcg gcggatcgcc gggagggtcg tgatggcgag
     2461 gttcttcatc gaccggccga tcgtcgcgtg ggtgttcgcg ctgatcgtca tgctcgccgg
     2521 cgtggtgtcg atcgagcgga tgccgatcgc gcagtacccg ccgatcgcgg cgcccgcgat
     2581 caccgcgacg tcgacctacc ccggcgcatc ggccgagacc gtcgacacga ccgtcaccca
     2641 ggtgatcgag cagcagatgc gcgggctcga caacctgcgc tacatgctgt cgaacagcga
     2701 ctccaccggc cagtcgcaga tcgagctgac gttcgagccg ggcaccgatc cgaacaccgc
     2761 gctgatccag atccag
//
LOCUS       DASVOR010001268         2127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421431,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001268 DASVOR010000000
VERSION     DASVOR010001268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2127
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421431"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VF516_24240"
     CDS             <1..1171
                     /locus_tag="VF516_24240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110873.1"
                     /translation="PRWLALVAQLSTRRGAQSLAELAGAAVRDPDSPDRLYDLGYALI
                     DAGAPAIAAAVLWRCLALVGDSEEVVCELVSALESALAYPDALAVLEDHAALRSRSFL
                     CRYLYAFNAAMSGRLDLTRAALAELSPESDETAGLRDTIQGICERADRVAGACPLDHR
                     DLRGWHHVLTGGLVIHQSPYGFDEPMHGRYAWLADSQPRIATGLDRLARLVAPLGLPC
                     IYTPPGRDHEILAHAAGMRLGMPIAPWPAIGVPAPGLIVLYDLAQLPDGEVARLIQRR
                     PDQVLFAHASPWTTDSPVAPDVTTLLYQTLVAPWAAHMILDPETRVLSEAAPDVRPAP
                     AIAAELAASPGLDDAELVADEPERWEALVARVWPLSPGPRSRLWAGGPVPSSRFT"
     gene            1195..1575
                     /locus_tag="VF516_24245"
     CDS             1195..1575
                     /locus_tag="VF516_24245"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110874.1"
                     /translation="MKKILVVEDDPVNQMILSDFLAANGYHTVAASSGPEGLERFERD
                     APDLLLVDIQLPRKNGFELLREIKDRPEGKATPILLMSAVYNDRDQSSRTIQLGTLAD
                     GYLTKPFDLVHLLAQVRQLLGEPS"
     gene            1585..>2127
                     /locus_tag="VF516_24250"
     CDS             1585..>2127
                     /locus_tag="VF516_24250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110875.1"
                     /translation="MSVRATLRWLFASVAEMGAPAHRRRLVQGVWSGYAVAIGFLVLA
                     AALSAFDLTGWHWVYFALVGGKLVTNSLAWLALSRRRAVLLAHVVNSSADLVALTCGI
                     YFTGGPHSPLLATYVIIIAVLALLANLGVTVLTAGIILVLFSSMIVLMAAGVLPPQPV
                     PGAPGAVPSPGLAVTAIAYCA"
BASE COUNT          247 a          803 c          752 g          325 t
ORIGIN      
        1 gccgcgctgg ctggcgctgg tcgcccagct ctcgacccgg cgcggcgcgc agtcgctcgc
       61 cgagctcgcc ggcgccgccg tccgggatcc ggacagcccg gaccggctct acgacctcgg
      121 ctacgcgctg atcgacgccg gcgcgccggc gatcgcggcc gccgtgctgt ggcgctgcct
      181 cgcgctggtc ggcgacagcg aggaggtggt gtgcgagctg gtgtccgcgc tggagagcgc
      241 gctggcctac cccgatgcgc tcgccgtgct cgaggaccac gcggcgctgc gctcgcggag
      301 ctttctgtgc cggtacctgt acgcgttcaa cgccgcgatg tcgggccggc tcgacctcac
      361 gcgcgccgcg ctggccgagc tgtcgcccga gtcggacgag accgccgggc tgcgcgacac
      421 gatccagggc atctgcgagc gcgccgatcg agtcgccggg gcgtgcccgc tcgaccaccg
      481 cgatctgcgc ggctggcatc acgtgctgac cggcggcctg gtgatccacc agtcgccgta
      541 cggcttcgac gagccgatgc acggccgcta cgcctggctc gccgactcgc agccgcggat
      601 cgcgaccggg ctcgatcggc tcgcccggct ggtcgcgccg ctcgggctgc cgtgcatcta
      661 cacgccgccc ggccgcgacc acgagatcct cgcacacgcc gccggcatgc ggctcggcat
      721 gccgatcgca ccgtggccgg cgatcggcgt cccggcgccg gggctgatcg tgctgtacga
      781 cctcgcgcag ctgccggacg gcgaggtcgc gcggctgatc cagcgccggc ccgatcaggt
      841 cctgttcgcg cacgcatcgc cgtggaccac cgacagcccg gtcgcgcccg acgtcaccac
      901 gctgctctac cagactctcg tagcgccgtg ggccgcgcac atgatcctcg atcccgagac
      961 ccgcgtgctg agcgaggccg ctcccgacgt ccggcccgcg ccggcgatcg ccgccgagct
     1021 cgcggccagc cccgggctcg acgacgcgga gctcgtcgcc gacgagccgg agcgctggga
     1081 ggcgctggtc gcacgtgtat ggccgctgtc gcctggcccg cggtcgcggc tgtgggccgg
     1141 cgggccggtc ccgtcgagtc gtttcacgta gcgcgtgcta cgctgcgacc agccgtgaag
     1201 aagatcctgg tcgtcgaaga tgatccggtg aaccagatga tcctctcgga ctttctggcc
     1261 gccaacggct accacaccgt cgccgcgtcg tcgggccccg agggcctcga gcggttcgag
     1321 cgcgacgccc ccgatctgct gctcgtcgac atccagctgc cgcgcaagaa cggcttcgag
     1381 ctgctgcgcg agatcaagga ccgccccgag ggcaaggcca cgccgatcct gctgatgagc
     1441 gcggtgtaca acgatcgcga ccaatcgagc cgcacgatcc agctcggcac gctggcggac
     1501 ggctacctga ccaagccgtt cgatctggtg cacctgctcg cgcaggtccg ccagctgctc
     1561 ggcgaaccgt cgtagcgggc cgcgatgtcg gtgcgcgcga cgctgcgctg gctgttcgcc
     1621 tcggtggccg agatgggcgc gccggcccac cgccggcgcc tggtccaggg cgtgtggtcg
     1681 ggctacgcgg tggcgatcgg cttcctggtg ctggcggcgg cgctgtcggc gttcgatctc
     1741 acgggctggc actgggtata cttcgcgctg gtcggcggca agctcgtcac caactcgctg
     1801 gcctggctgg cgctgagccg ccggcgcgcc gtgctgctcg cccacgtcgt caactcctcg
     1861 gccgatctcg tcgcgctgac ctgcgggatc tacttcaccg gcgggccgca cagcccgctg
     1921 ctcgcgacct acgtgatcat catcgcggtg ctcgcgctgc tcgccaacct cggcgtgacc
     1981 gtgctgaccg ccgggatcat cctggtgctg ttctcgtcga tgatcgtgct gatggccgcc
     2041 ggcgtgctgc cgccccagcc ggtgccgggc gcgcccggcg cggtcccgtc gcccgggctg
     2101 gcggtcaccg cgatcgccta ctgcgcg
//
LOCUS       DASVOR010001269         5989 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_568656,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001269 DASVOR010000000
VERSION     DASVOR010001269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568656"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VF516_24255"
     CDS             complement(<1..199)
                     /locus_tag="VF516_24255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110876.1"
                     /translation="MATSGFAGLLSRRSTLAGSASLVLHGGVLVLAVALVGARVARVA
                     RPPRPVAWTAVEVISPLPPAPA"
     gene            complement(234..536)
                     /locus_tag="VF516_24260"
     CDS             complement(234..536)
                     /locus_tag="VF516_24260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110877.1"
                     /translation="MLSQNTPSQITQAAEDSMASDGCSSAVLDLAAFSEGNLSDEGLR
                     RLAGHLQQCSPCKALFASLAKDAESALRAGGHDVMSGIAIGNGNHPASPVRTNSGE"
     gene            complement(1040..1876)
                     /locus_tag="VF516_24265"
     CDS             complement(1040..1876)
                     /locus_tag="VF516_24265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110878.1"
                     /translation="MRASTRHQLDELLGNSGFLKTVDLITKEVYHVWNVSDRDAYGVV
                     LSAIGEPATLACVHAALSPNQNIALAKVIIRRRVIDLLRKDARPNNHCSLSPPAADGT
                     EPDCAPGSFGDPLESTPRGQLEVRQIVNRVRTALDEFAAQGDKQQQQAQLLQRYVLDE
                     VGYADLSVELTCSKNALRVRVHKAITALRKYVQSCHPELEQLLEGRSPRPPDGHDAGE
                     APAILDEAVDRPREPRLGLTGEDHASACPARTAPPLLLAANRKSGVWARGSDHAIKLP
                     VT"
     gene            2419..2793
                     /locus_tag="VF516_24270"
     CDS             2419..2793
                     /locus_tag="VF516_24270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110879.1"
                     /translation="MTWCQVFHHIEHTLRLGIGPLSDDKEVRRDIAVRVLQKLEANGY
                     KHLREWVDRQRRGTDSCSWWGFINMVARRCAIDYARTSSRNVARRGEPFEWVRVEPED
                     PAVLVERMESSKAQLRSRRDRS"
     gene            2865..3593
                     /locus_tag="VF516_24275"
     CDS             2865..3593
                     /locus_tag="VF516_24275"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8110880.1"
                     /translation="MIERDIDIRTPDGAMNSFVVHPEEGGPFPVVLFYMDAPGKREEL
                     HDMARRIAAVGYYVVLPNLYYRTTREFVMVRDEDGFKRMFELLRGLSIEMMMRDTAAL
                     LAFVDADPAARARSLGAVGYCMSGPFVFAAAARFPDRIQCAASIYGAGLVTDRPDSPH
                     LTMKQIRGEMYFACAEIDKYAPRDQIDALEAQLEASGIRYRLEWYPGAEHGFAFPQRA
                     GIYHKPSAERHWERLFALFRRNLG"
     gene            complement(3622..4767)
                     /locus_tag="VF516_24280"
     CDS             complement(3622..4767)
                     /locus_tag="VF516_24280"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DSD1 family PLP-dependent enzyme"
                     /protein_id="HEX8110881.1"
                     /translation="MTDDLRLHGSLIGRAARDRLATPALVIDRDALDRNLARMAEFAR
                     GHGTALRPHAKTHKSVDIARRQIAAGAIGVCCAKLGEAEALADGGIPRLLITSPVVAE
                     PAIRRLVELRRRCDELIAVVDHPANAAALAAAASHGEPLDVLIDIDPGIHRTGVASAA
                     AALELAQRIRTLPELRYRGVQFYCGVQQHIARVAEREAAIRERMQYLRSVIALLSEHQ
                     VAPAIITGAGTGTHRIDAALGLYTEWQVGSYVFMDRQYAECELVQGGDGAAPFEYALF
                     VETTVVSANHRGMATIDAGFKALATDGGPPAIVSGAPAGASYRFMGDEHGAIIDPAGA
                     HVWQLGDRVRLAVPHCDPTVNLYDSYHVLSGDTLEAIWPVSARGRSR"
     gene            complement(4764..>5989)
                     /locus_tag="VF516_24285"
     CDS             complement(4764..>5989)
                     /locus_tag="VF516_24285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HEX8110882.1"
                     /translation="ELAAVVRAATRLRVVGAGHSFSPLCATDGTLVRLDELDLDDGDA
                     SPVIYLAPDGRSAWAPAGWSLRRVAAALWELGRSLPNQGDIDAQALAGAIATGTHGTG
                     AELGSLASFARAFRLMLADGSIVECSRDAEPELFEAQRLSLGLLGIATRIRIDVAPAY
                     RLEQRIARVGLDEVCERFAEIAGGHRHAEFFVFPYADDAILKTLHPTDDASPFRPPRS
                     DGEDEFQRICDLCCWLPSLTGVMQHRMMAAIHGSRRVGPAHLIFPSERRVRFEEMEYE
                     LPRDAGLAALREAIRWIRRRRLPVLFPFEFRWVAADDIWLSPFRAPSASISIHQYAGR
                     RWQELFAEVEPMFRAHGGRPHWGKRHSLTARDVFALYPRAGAFCTVRHRVDPGRKFAN
                     DALTRLFDLEAPERP"
BASE COUNT          858 a         2136 c         2127 g          868 t
ORIGIN      
        1 cggccggggc tggcggcagc ggcgagatca cctcgaccgc ggtccacgcg accggacgcg
       61 gcggccgggc aacgcgggca acgcgggctc cgaccagcgc gaccgcgagc accagcacgc
      121 cgccgtgcag gacgagcgac gccgatccgg ccagcgtcga ccgccgggac agcagcccgg
      181 caaagcccga ggtcgccatg gtcccgaatc tatcaccggg cggcagcgcc cggctattcg
      241 ccgctgttcg tccggaccgg cgatgccggg tggttgccgt ttccgatggc gattccggac
      301 atcacgtcgt gcccccctgc gcgcagcgcg ctctcggcgt ccttggccag cgatgcaaac
      361 agcgccttgc agggcgagca ttgctgcaga tgaccggcca gccgccggag cccttcgtcc
      421 gagaggttcc cttcgctgaa cgcggcgaga tcgagcacgg cggacgagca accgtcggac
      481 gccatggagt cctccgcagc ttgcgtgatc tgcgacggcg tgttctgcga cagcatgtcc
      541 ttctgttgtg aagccatgtg cctctccttg tgtggggggc tccatcggcg acgcggcccc
      601 tgctacagga gtaccgtcgt tcggggccct cctccaccgt cggatggtcc ccggcgtcgc
      661 atttctggcc ggataggttc ctcccacccc ctgagtcgag gccggccgga gccgtgttag
      721 ccaggagcgt cccattgtgg accgcgaacc gaccggggcg ccccgacatg cacgttccgg
      781 accgatgtcc ggacgagcgc aagtcctcga tcccgcagcg gccgagacgg cgcatcacgc
      841 caccgacatg gcggccgccg acctgtgcgg gtcgcagggt cccgccggtg tgccggagat
      901 ctgctctccg cgccgttccg gatgcgggca tccgggcgca ccccgagcgc ctcgaacccg
      961 gccgaccgcc cgtgcgctgc cgcagttcgc tggcgagacg tcggcaaccg cgccgcgacg
     1021 cgacaccgcg ggcggcgggt cacgtgaccg gcagcttgat cgcgtgatcg ctgccgcgcg
     1081 cccagacacc gctcttgcgg ttcgcggcga gcagcaaggg cggcgcggtc ctcgccgggc
     1141 acgcgctggc gtggtcctcg ccggtgaggc cgagccgcgg ctcccggggc cgatccacgg
     1201 cctcgtccag gatggccggc gcctcccccg catcgtgacc gtcgggcggc ctcggcgatc
     1261 gtccctcgag cagctgctcg agctcggggt gacagctctg cacgtacttg cgaagcgccg
     1321 tgatggcctt gtgcacacgg acgcgcagcg cgttcttcga gcacgtcagc tccacgctga
     1381 ggtctgcata tccgacctcg tcgagcacgt agcgttgcag cagctgcgcc tgctgttgct
     1441 gcttgtcccc ttgcgcggca aactcgtcga gcgccgtccg gaccctgttg acgatctggc
     1501 ggacctcgag ctgcccccgc ggcgtcgatt ccagcggatc gccgaagctg ccgggtgcgc
     1561 aatccggctc cgtgccgtcg gccgccggcg gcgacagcga gcaatgattg ttgggtctgg
     1621 cgtccttgcg cagcagatcg atcacacgcc gtcggatgat caccttcgcg agcgcgatgt
     1681 tctgattggg cgacagcgcc gcgtgcacgc acgcgagcgt cgccggctcg ccgatcgcgg
     1741 acagcacgac gccgtaggca tcccggtccg acacgttcca gacgtgatag acctccttcg
     1801 tgatcagatc gaccgtcttc aggaagccgc tgttgcccag cagctcgtcc agctgatgtc
     1861 tcgttgaagc acgcacctcg tcaccctcct cgcgccgccc gcccagccga tgtctcgttg
     1921 aagaacgcat gcggctacct tcggctgata gggacgacag gctctccgcg atgccgcaac
     1981 atgcggcagc tgcagcccgt gcggcacggt ggcttcgtcc cggcgcgcaa cgcgtgtcgc
     2041 aagatctcgc cggtcctggg tgaatctcgc gaacggccga cctctcctgc cccagcggta
     2101 gatcgcgctc actcatggag gacccgccgg atcgtcggga tctgctcgcc cggatcgccg
     2161 ctagaaggtg acccacatgc cgcaacggcg ggcgaagctc cgcgaccgcg gacgcgcgca
     2221 gcccgggcga ggccgaccga gctccggtct cccgaccggc acggattgcc gtcgctgtac
     2281 ggcaactgtc gtccttcttc ggagtcagcc ggatcacgcc aacccaggag gattgcgatg
     2341 acagcgtcta cgcgttggga tgggcaatgg gtcaagatca tcgaggatct cctggctggc
     2401 gatcgggttg cccgcgatat gacgtggtgt caggtcttcc atcacatcga gcacacgctc
     2461 cggctgggca tcggcccgct gagcgacgac aaggaggtcc gtcgcgacat cgcggttcgc
     2521 gtgctccaga agctggaagc gaatggctac aagcatctcc gcgagtgggt ggatcgccag
     2581 cggcgtggca ccgacagctg ttcctggtgg ggattcatca acatggtggc gaggcgctgc
     2641 gccatcgact acgcgcggac cagcagccgc aacgtggccc gccgcggcga gccgttcgag
     2701 tgggtgcggg tcgagcccga agatccggcg gtgctggtcg agcggatgga gagctcgaag
     2761 gcacagctcc gctcccgccg cgatcggagc tgatcgacgt ctcggtcgcg tcccggtcgg
     2821 ccggcggttg gtcgcgatcg cgccggctgg tatcgtgcgc acccatgatc gagcgcgaca
     2881 tcgacatccg gacgcccgat ggcgccatga acagcttcgt ggtccacccc gaggagggcg
     2941 ggccgttccc cgtggtgctg ttctacatgg acgcgcccgg caagcgcgag gagctccacg
     3001 acatggcgcg ccgcatcgcg gcggtcggct actacgtcgt gctgcccaac ctgtattacc
     3061 gcacgacccg cgagttcgtg atggtgcgcg acgaggacgg gttcaagcgg atgttcgagc
     3121 tgctgcgcgg gctgtcgatc gagatgatga tgcgcgacac cgccgcgctg ctcgcgttcg
     3181 tcgacgcaga ccccgccgcc cgcgcgcgat cgctcggcgc cgtcggctac tgcatgagcg
     3241 gcccgttcgt gttcgccgcc gcggcgcggt tccccgatcg catccagtgc gcggcgtcga
     3301 tctacggcgc gggcctggtc accgatcgcc ccgactcgcc gcacctgacg atgaagcaga
     3361 tccgcggcga gatgtacttc gcgtgcgccg agatcgacaa gtacgcgccc agggaccaga
     3421 tcgacgcgct ggaggcccag ctcgaggcgt ccgggatccg ctatcgcctc gagtggtacc
     3481 cgggcgccga gcacggcttc gcgttcccgc agcgcgccgg catctaccac aagccgagcg
     3541 ccgagcggca ctgggagcgg ctgttcgcgc tgttccgcag gaacctcggc tgacgccggc
     3601 gccggcgcgt caccgcgccg atcaccgtga tcggccgcgc gcgctgaccg gccagatcgc
     3661 ctcgagcgtg tcgcccgaca gcacgtggta gctgtcgtac aggttgaccg tcggatcgca
     3721 gtgcggcacg gcgaggcgca cccggtcgcc gagctgccag acgtgcgcgc ccgctggatc
     3781 gatgatcgcg ccgtgctcgt cgcccatgaa ccgatagctc gcgccggccg gcgcgccgga
     3841 cacgatcgcg ggcggcccgc cgtcggtggc gagcgccttg aagccggcgt cgatcgtcgc
     3901 catgccgcgg tggttggcgc tcaccacggt ggtctcgacg aacagtgcgt actcgaacgg
     3961 cgcggcgcca tcgccgccct gcacgagctc gcactcggcg tactggcggt ccatgaacac
     4021 gtacgagccg acctgccact cggtgtagag cccgagcgcg gcgtcgatcc ggtgcgtgcc
     4081 ggtgccggcg ccggtgatga tcgccggggc cacctggtgc tccgacagca gcgcgatcac
     4141 cgaccgcagg tactgcatgc gctcgcggat cgccgcctcg cgctcggcga cccgcgcgat
     4201 gtgctgctgg acgccgcagt agaactggac gccgcgatag cgcagctcgg gcagcgtgcg
     4261 gatccgctgc gcgagctcga gcgccgcggc ggccgacgcc acgccggtgc gatggatccc
     4321 cggatcgatg tcgatcagca cgtcgagcgg ctcgccgtgc gaggccgcgg cggccagcgc
     4381 ggcggcgttg gccgggtgat cgaccacggc gatcagctcg tcgcagcgcc ggcggagctc
     4441 gaccaggcgg cggatcgccg gctcggcgac caccggcgag gtgatcagca gccgggggat
     4501 gccgccgtcg gcgagcgcct cggcctcgcc gagcttggcg cagcacacgc cgatcgcgcc
     4561 ggcggcgatc tggcggcgcg cgatgtcgac gctcttgtgg gtcttggcgt gcgggcgcag
     4621 cgcggtgccg tggccgcgcg cgaactcggc catccgcgcg aggttgcggt cgagcgcgtc
     4681 gcggtcgatc accagcgccg gggtggcgag ccgatcgcgc gcggcgcggc cgatcagcga
     4741 gccatggagt cgtaggtcgt cggtcatggg cgttccgggg cctccaggtc gaacaggcga
     4801 gtcagggcat cattcgcgaa cttgcgcccg ggatccaccc ggtggcgcac cgtgcagaac
     4861 gcgccggccc tggggtagag cgcgaacacg tcgcgcgcgg tgagcgagtg gcgcttgccc
     4921 cagtgcggcc ggccgccgtg cgcgcggaac atcggctcga cctcggcgaa cagctcctgc
     4981 cagcgccgcc ccgcgtactg gtggatcgag atcgacgcgc tcggcgcgcg gaacggcgac
     5041 agccagatgt cgtcggccgc gacccagcgg aactcgaacg ggaacagcac ggggagccgg
     5101 cgccgccgga tccagcggat cgcctcgcgc agcgcggcca gccccgcgtc gcgcggcagc
     5161 tcgtactcca tctcctcgaa ccgcacccgg cgctccgacg ggaagatcag gtgcgcgggg
     5221 ccgacccggc gcgagccgtg gatcgccgcc atcatccggt gctgcatcac gccggtcagc
     5281 gacggcagcc agcagcacag atcgcagatc cgctggaact cgtcctcgcc gtcgctgcgc
     5341 ggcgggcgga acgggctggc gtcgtcggtc ggatgtaacg ttttcaggat cgcgtcgtcg
     5401 gcgtacggga acacgaagaa ctcggcgtgg cggtgcccac ccgcgatctc ggcgaaccgc
     5461 tcgcacacct cgtcgaggcc gacccgcgcg atccgctgct ccaggcgata cgccggcgcg
     5521 acgtcgatcc ggatccgggt cgcgatgccg agcaacccga gcgacagccg ctgggcctcg
     5581 aacagctcgg gctccgcgtc gcggctgcac tcgacgatcg agccgtcggc gagcatcagc
     5641 cggaacgcgc gcgcgaagct cgcgaggctg ccgagctcgg cgccggtgcc gtgcgtgccg
     5701 gtggcgatcg cgccggccag cgcctgcgcg tcgatgtcgc cctggttggg cagcgagcgg
     5761 ccgagctccc acagggccgc ggcgacccgc cgcaggctcc agccggccgg cgcccaggcg
     5821 ctgcggccgt cgggtgcgag gtagatcaca ggggaggcgt ccccatcgtc caggtcgagc
     5881 tcatcgagcc ggaccagcgt gccgtcggtc gcgcacagcg gggagaacga gtggccggcg
     5941 ccgacgacgc gtagccgggt ggcggcgcgc accaccgccg cgagctcgt
//
LOCUS       DASVOR010001270         3027 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_275307,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001270 DASVOR010000000
VERSION     DASVOR010001270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275307"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..533
                     /locus_tag="VF516_24290"
     CDS             <1..533
                     /locus_tag="VF516_24290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110883.1"
                     /translation="PGGRMFSVGPAIDLPAIGRTLEDVPGRRLRRVLLDLGPDPDPDP
                     AADPAPRLRVFDDRLFDTALGADCRPRSIRGAIRCLPVAVTTATQVFTAGCTAPVLVA
                     EVPQHACEPPTYATTNRPFQIRPVGDLAPGPLFRTDGTTCAPYAGAPGNELRALGAPI
                     DPTTFLGAIYYGERSL"
     gene            complement(625..2703)
                     /locus_tag="VF516_24295"
     CDS             complement(625..2703)
                     /locus_tag="VF516_24295"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8110884.1"
                     /translation="MHARLALALTLLGCAAAARPLPSYPPSRATDFKETLHGVELADP
                     YRWLEDDGSPETRTWIDAQNRYTHALLDGEPALPRIRARLEALARVETQSAPFEAGGR
                     CFVWTRTPKDELAILTVREGATAEARVLLDPNPLSPDHTTQAGLESVSRDGRLMVYSI
                     RRSGKDESELRVRDVASGADADDVMPEGLYRGASLDGDARGFYYALQDRATGSRIRYH
                     ALGTPASADREVFGSGYGPSQWVNAFVSENGRHLVFTVSHGWQRNEIWVQDLPARGPP
                     QPVVKDLDAHFDLEYAGDRLIVQTDWQAPRGRIVEIDPAAPAPERWREIVPPRDDAIA
                     GFRAVGGKLLVRTLHDVSARLELFSVEGKPLGEVELPGRGSVGAINGRWESGEVFFDF
                     TSYTVPRATYRLDLATGNVARWWRPQVPFDGEAYETEQVWYPSRDGTKIPMYVIHRKG
                     LVRDGRTPALLYGYGGFGVALLPSFSSRYAWFLEAGGIVAVANLRGGSEFGEAWHKAG
                     MLDKKQNVFDDFIAAGEHLARERYTSPERLAIAGGSNGGLLVGAALTQRPDLFRAVLC
                     EFPDLDLVAFPRLKNNSPPALLEYGDARKPDEFSFIRTWSPYQNVRRDTRYPAVFLTT
                     GDADTRVPPAQARKMTARLQAATTSSRPVVLLYDTKAGHAGGRSLTKVIDDTTMEMAF
                     LVRELGMTVP"
     gene            2698..>3027
                     /locus_tag="VF516_24300"
     CDS             2698..>3027
                     /locus_tag="VF516_24300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110885.1"
                     /translation="MHAPDDITADASPISAGTPMRGLARRSSGRLLVAGRVLLTGNRY
                     PCTFRFQLESCPYVVGHLVPEGPELSAPVYRRTGSACSPADAPAGGRMFAVGPAIELP
                     AIGRALED"
BASE COUNT          403 a         1103 c         1081 g          440 t
ORIGIN      
        1 cccccggcgg ccggatgttc tcggtcggcc ccgcgatcga cctgcccgcg atcggccgca
       61 cgctggagga cgtccccggt cgccgcctgc gccgcgtcct cctcgacctc gggcccgatc
      121 ccgaccccga tccggccgcc gaccccgccc cccggctccg cgtgttcgac gaccggctgt
      181 tcgacaccgc gctcggcgcc gactgccggc cgcgcagcat ccgcggcgcg atccgctgcc
      241 tcccggtcgc cgtcaccacc gcgacgcagg tgttcaccgc cggctgcacc gcgccggtgc
      301 tggtcgccga ggtcccgcag cacgcctgcg agccgccgac ctacgcgacc acgaaccggc
      361 cgttccagat ccggccggtc ggcgatctcg cgcccggtcc actgttccgg actgacggca
      421 cgacctgcgc gccgtacgcc ggcgccccgg gcaacgagct gcgcgcgctc ggcgcgccga
      481 tcgatccgac gacgttcctc ggcgcgatct actacggcga gcgctcgctg taggcccgga
      541 tcattcacac gacgtctcgg tcgactcatt ccgctcgctc acgctcgcga taggcaagcg
      601 ccgacgccct cgccgaccat cgggtcaggg tacggtcatg ccgagctcgc gcacaagaaa
      661 cgccatctcc atcgtcgtat cgtcaatgac cttggtgagc gagcgcccgc cggcgtggcc
      721 ggccttggtg tcgtagagga gcacgacggg gcgactcgac gtggtcgcgg cctgcaggcg
      781 tgcggtcatc tttcgcgcct gcgccggcgg gacgcgcgtg tcggcatcac ccgtggtgag
      841 gaagaccgcc gggtagcgcg tgtcgcggcg cacgttctgg tacggcgacc aggtgcggat
      901 gaaggagaac tcgtcgggct tgcgcgcatc gccgtattcg aggagcgcgg gcggactgtt
      961 gttcttgagg cgcgggaacg cgacgagatc gaggtcgggg aactcgcaga ggacggcacg
     1021 gaacagatcg gggcgctggg tcagcgcggc gccgacgagg agaccgccgt tgcttccgcc
     1081 ggcgatggcc agccgctcgg ggctggtgta gcgctcgcgc gccaggtgct cgccggccgc
     1141 gatgaagtcg tcgaacacgt tctgcttctt gtccagcatg cccgccttgt gccaggcctc
     1201 gccgaactcg ctccccccgc gcaggttggc cacagcgacg attccgccgg cctcgaggaa
     1261 ccaggcgtag cgcgagctga acgagggcag gagggccacg ccgaagccgc catacccgta
     1321 gagcagcgcc ggcgtccgtc cgtcgcggac cagccccttc cggtggatga cgtacatggg
     1381 gatcttggtg ccgtcgcgcg aggggtacca gacctgctcg gtttcgtacg cctcgccgtc
     1441 gaagggcacc tggggacgcc accagcgggc tacgttgccc gtcgcgaggt cgaggcggta
     1501 ggtcgcgcgc ggcacggtgt agctggtgaa gtcgaagaag acctcgccgc tctcccaccg
     1561 gccgttgatc gcacccaccg agccgcgccc gggcagctcc acctcgccga gcggcttgcc
     1621 ctcgacggag aacagctcga ggcgtgcgct cacgtcgtgg agcgtgcgca ccagcagctt
     1681 gccgccgacg gcccggaagc cagcgatggc gtcgtcgcgc gggggcacga tctcgcgcca
     1741 gcgctcgggc gccggcgcag cggggtcgat ctcgacgatg cgcccgcgcg gcgcctgcca
     1801 gtcggtctgc acgatcaggc ggtcgccggc atactcaaga tcgaagtggg cgtcgagatc
     1861 cttcacgacc ggctgcggcg ggcccctggc ggggaggtcc tggacccaga tctcgttgcg
     1921 ctgccagccg tgcgagaccg tgaagaccag gtggcgcccg ttctccgaga cgaacgcgtt
     1981 cacccactgg ctcgggccgt agccgctgcc gaacacctcg cgatccgccg acgccggcgt
     2041 gcccagcgcg tggtagcgga tgcggcttcc ggtggcgcgg tcctgcagcg cgtagtagaa
     2101 gccgcgcgca tcgccgtcga gggacgcgcc gcggtacagg ccctccggca tcacgtcgtc
     2161 ggcgtcggcg ccgctggcca cgtcgcgcac gcgcagctcg ctctcgtcct tgcccgagcg
     2221 ccggatgctg tagaccatga ggcgcccgtc ccgggacacg ctctccaggc ccgcctgcgt
     2281 ggtgtgatcg gggctgagcg ggttcggatc gaggagcacg cgcgcctccg cggtcgcgcc
     2341 ctcgcgcacc gtgaggatgg cgagctcgtc cttcggcgtt cgcgtccaga cgaagcagcg
     2401 accgccggcc tcgaacggcg cgctctgtgt ctcgacgcgg gccagcgcct cgagccgagc
     2461 gcggatgcgc ggcagcgccg gctcgccgtc gaggagggcg tgggtgtagc ggttctgcgc
     2521 gtcgatccag gtgcgcgtct ccgggctccc gtcgtcctcg agccagcggt aggggtcagc
     2581 gagctcgacg ccgtggagcg tctccttgaa gtcggtagct cgcgacggcg ggtaagaagg
     2641 gagtggccgc gcggccgcag cgcagccgag cagggtcagg gcgagcgcaa gtcgggcgtg
     2701 catgcgcccg acgatatcac cgccgacgca tcgccgatct ccgccgggac cccgatgcgg
     2761 gggctcgcgc gccgcagctc cggccggcta cttgtcgcag gccgcgtact gctcacaggt
     2821 aatcgctacc cctgtacatt ccgctttcag cttgagtcct gcccgtacgt cgtcgggcat
     2881 ctggttccag agggccccga gctgtcggcg ccggtctatc gcaggaccgg cagcgcgtgc
     2941 agccccgccg acgcgcccgc cggcggccgg atgttcgcgg tcggcccggc gatcgagctg
     3001 cccgcgatcg gccgcgcgct ggaggac
//
LOCUS       DASVOR010001271         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_129744,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001271 DASVOR010000000
VERSION     DASVOR010001271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129744"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..926
                     /locus_tag="VF516_24305"
     CDS             24..926
                     /locus_tag="VF516_24305"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8110886.1"
                     /translation="MERSRLSPFGLVLLALVSVAWGLSWASIKIVLAEVPPLTFRAIC
                     LVVGGAGMLALARLAGQSLHVPARAWGRLLAVAACNIVGWNTFKIYGIASLPSGRAAL
                     IGYTMPMWSTLFSVWLLGERLTVRRGASLVLGMAGVVVLLAAGATGMASATTGVVLML
                     ASAASWGLSVVLLKRFALPAPTACLTGWMMLAGSVPFVAGAVALEHDRWQPVTTTVGL
                     GLAYSVVIAFMFGYWAWNRVVLMVPVAVSSVSILAVPVISLLSGAWLLHEPLTWHELV
                     AGACILGAIALVLGSPAARRAGDR"
     gene            complement(1018..1809)
                     /locus_tag="VF516_24310"
     CDS             complement(1018..1809)
                     /locus_tag="VF516_24310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110887.1"
                     /translation="MFLHRLVVVSPLVLSASLARADVADDSANEGAASAHAAANDDSF
                     TPGLTHATGPLLAGVAATATLDGASHTNTVDLNGEVHVWGPARLVLRVDNVFAANGGK
                     ARPGIGAAVQLLDERKHGVASSAYVSYRAEGFTEAEGEIEGLVSFGKQLGPVHGTLNL
                     AYGQDPDNVERDGEIALGLHVEPIHGLFTGVVGRFRDALGSNGDKGTGILRDGLGAAT
                     ATYVIGRIGVTASAGIAAVKTLNAATQTTSTQTGAQAAFSVGTVF"
     gene            2202..>2609
                     /locus_tag="VF516_24315"
     CDS             2202..>2609
                     /locus_tag="VF516_24315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110888.1"
                     /translation="MQVIKPDAQDVSPPLSELAKRPVPFAVERQHEAEPWRAIPHKQL
                     EASGPVVDPVVQTAIGGPDIPTPITTFEGMGTGLPGFTVQSAPPDTDGDIGPNHYVQI
                     VNSGVTIFSRTGAVLMGPVNTNTLWNGFNGACAT"
BASE COUNT          366 a          876 c          932 g          435 t
ORIGIN      
        1 cgccgcggag tacgctgcgc gccgtggaac gctcccgcct gtcgccgttc ggactggtcc
       61 tgctcgcgct ggtcagcgtc gcctgggggc tgtcgtgggc gtcgatcaag atcgtgctcg
      121 ccgaggtccc gccgctgacg ttccgcgcga tctgcctggt cgtgggcggc gccgggatgc
      181 tcgcgctggc ccgcctcgcc gggcagtcgc tgcacgtgcc ggcgcgcgcg tggggccgcc
      241 tgctcgcggt cgcggcgtgc aacatcgtcg ggtggaacac gttcaagatc tatggcatcg
      301 cgagcttgcc gtcggggcgg gccgcgctga tcggctacac gatgccgatg tggagcacgc
      361 tgttctcggt gtggctgctc ggcgagcggc tcacggtgcg gcgcggcgcc tcgctcgtgc
      421 tggggatggc cggcgtcgtg gtcctgctcg ccgccggcgc cacgggcatg gcgagcgcca
      481 ccaccggcgt ggtgctgatg ctggcgtccg cggcgtcctg ggggctcagc gtggtgctgc
      541 tcaagcggtt cgcgctgccg gccccgactg cgtgcctgac cggctggatg atgctggccg
      601 gcagcgtgcc gttcgtcgca ggcgcggtgg ccctggagca cgaccgctgg cagccggtca
      661 ccacgaccgt cgggctcggc ctggcctaca gcgtggtcat cgcgttcatg ttcggctact
      721 gggcgtggaa ccgggtcgtg ctcatggtgc cggtggcggt gtcatcggtc tcgatcctcg
      781 cggtgccggt gatcagcttg ctgagcggcg cgtggctgct ccacgagccg ctgacctggc
      841 acgagctcgt cgccggcgcg tgcatccttg gcgcgatcgc gctcgtgctc ggctcacccg
      901 ccgctcggcg cgccggcgat cggtagtcgc agatcggagc ggcagcggcc gcgcgccggt
      961 tcactgcggg aagacgacga cgagcccgtc ggtgttgccc gcggcggtga ccgagagcta
     1021 gaacaccgtg ccgaccgaga acgcggcctg cgcgccggtc tgcgtgctgg tggtctgcgt
     1081 ggcggcattc aaggtcttga ccgcggcgat ccccgcgctc gcggtcacgc cgatccggcc
     1141 gatcacgtag gtcgcggtgg cggcgccgag cccgtcgcgc aggatcccgg tgcccttgtc
     1201 gccgttggac ccgagcgcgt cgcggaaccg gccgaccacg ccggtgaaca ggccgtggat
     1261 cggctcgacg tgcaggccga gggcgatctc gccgtcgcgc tcgacgttgt cgggatcctg
     1321 gccgtaggcg aggttgagcg tgccgtggac cggcccgagc tgcttgccga acgagaccag
     1381 gccctcgatc tcgccctcgg cctcggtgaa gccctcggcc ctgtacgaca cgtaggccga
     1441 cgacgccacg ccgtgcttgc gctcgtcgag cagctggacc gccgcgccga tccccgggcg
     1501 cgccttgcca ccgttggcgg caaacacgtt gtcgacccgc agcaccaggc gggcgggtcc
     1561 ccagacgtgg acctcgccgt tgaggtcgac ggtgttggtg tgggatgcgc catcgagcgt
     1621 cgcggtcgcc gccaccccgg ccagcagcgg tccggtcgcg tgggtcagcc cgggagtgaa
     1681 cgagtcgtca ttggccgcgg catgggcgct ggcggctccc tcgttcgcgc tgtcgtccgc
     1741 cacatcggcg cgggcgagcg aggcggacag gacgagcggt gacacgacca ccagacggtg
     1801 caggaacatg taccgcacga taggcgatcg cgacccgggg tgtcgatcta ggttccctgg
     1861 cagcgctgcg acgtcaagtt cgatcacgat gtagttaaaa tcactactgg tacgtgagaa
     1921 gttttctgtg gaactacatg cactaatcgc gcatttttat tacgtaaaat tggtcagaca
     1981 cgtcacaact taaggtagtt acgagtagga taacgttaag actcgatccg gccagcggga
     2041 aacccctagc agcgacgatg tcggcaaacg tagatggctt ctgccgagct ttacccatcc
     2101 ggaggccgac acagatgaag ttgcgatcga gagcatggat ccttggcgtt cttgccgcgt
     2161 ccctcgccgc gtgtgcgacg ggcgggtcgg acgattcaaa tgtgcaagtc atcaagccag
     2221 acgcgcagga cgtctcgccg ccgctgagcg agctcgccaa gcggccggtg ccgttcgcgg
     2281 tcgagcgcca gcacgaagcc gaaccgtggc gcgcgatccc gcacaagcag ctcgaggcgt
     2341 ccggcccggt ggtcgatccc gtcgtgcaga ccgcgatcgg cggccccgac atcccgactc
     2401 cgatcaccac gttcgagggc atgggcaccg ggctgcccgg cttcacggtg cagtcggcgc
     2461 cgccggacac cgacggtgac atcggcccca accactacgt ccagatcgtc aactcgggcg
     2521 tcacgatctt cagccgcacc ggcgcggtgc tgatgggccc ggtcaacacc aacacgctgt
     2581 ggaacggctt caacggcgcg tgcgcgacg
//
LOCUS       DASVOR010001272         6919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_334301,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001272 DASVOR010000000
VERSION     DASVOR010001272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334301"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1541)
                     /locus_tag="VF516_24320"
     CDS             complement(<1..1541)
                     /locus_tag="VF516_24320"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8110889.1"
                     /translation="MSDGRGAQASELEADLAREIAGEVRFDAGSRSIYAHDASNYRQV
                     PIGVVIPRNAEDVEHALRTCRSHGVPVVSRGGGTSLAGQTVNRAVVFDFSKYMNEVLE
                     VDTGRWRARVQPGCVLDDLRAALGKHGLTYGPDPATHNHCTLGGMVGNNSCGIHSVMG
                     ELYGPGARTSDHVESLEIVTYDGLRMRVGATSEDELARRIAAGGRAGEIYAALARLRD
                     RYAELLRTRFAPIPRRVSGYNLDELLPERGFHVARALVGSEATCVTVLEATLAVYPAR
                     AERVLCVLGYRDVYEAGDHIPQIRGFRPVGLEGLDQRLVDDMRATGTHAENIGLLPDG
                     KGWLLVEFGADTQAEALARARGMIEAVRREPNAPSIKLFDRMDEAHKVWEVREAGLGA
                     TAFIPGHRDFWPGWEDSAVAPDRVGPYLRELRQLMQHHGLEAALYGHFGQGCIHCRID
                     FELSTPEGIATYRRFTDAAADLCVRYGGSISGEHGDGQSRGDLLVKQFGPELVEAFRE
                     FKRIW"
     gene            1720..2154
                     /locus_tag="VF516_24325"
     CDS             1720..2154
                     /locus_tag="VF516_24325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110890.1"
                     /translation="MPIDPPTTPPTSPAPPAPPARPARVDPEIWPYGWPRWIPIALGA
                     WLFLSAFLWPHGTGAAINSWIIGLLIVIASLIALRMPWMRWVVTALAVWLFLSTLAIA
                     ATRATLWNNLIVAILVFLASLVPEGLDPLLSRPRRGARGAVR"
     gene            complement(2168..3004)
                     /locus_tag="VF516_24330"
     CDS             complement(2168..3004)
                     /locus_tag="VF516_24330"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8110891.1"
                     /translation="MGSNPGPSCPMEAFVEVSLGMLLDLAGAGSGLPDLDDRLVEPGT
                     RHEMLDGELVHVPPADAPHAERQFQLCPLIEAHTGAEFQAACELLTRISRRDDLAPDV
                     SVYPAARDPVTGRRPLEQLAFEVVSTQSLGDAGRKAAKLVARGVRRVFAIDVERSRAL
                     EWSTALDTWSMLDAAGHITDPAFAVPLPIETLIHSARTDDAVARALVVKHNPVIEAIR
                     AEGRVEGLAEAIVAMLSARGVPTSPVERDRILAERDPARLGRWITRAIECTSVAELLS
                     EP"
     gene            3309..3509
                     /locus_tag="VF516_24335"
     CDS             3309..3509
                     /locus_tag="VF516_24335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110892.1"
                     /translation="MTIAYTDDEVVTLLRFAFRKDPHHLGASALLRAIWVNDDSLAVD
                     QATACGQSMGRPHSSVRFGACR"
     gene            3514..4044
                     /locus_tag="VF516_24340"
     CDS             3514..4044
                     /locus_tag="VF516_24340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110893.1"
                     /translation="MRKPCDPQALLARCRKPGSNSYDDARLDNAARQVADAIERGADE
                     HWKAPAMAAAYRILEKSSWPRSPQIEAAARRLLSRAPLKVVVGALHLEILRAKTRQIA
                     LDRARRLSTDIVADADRYVSNWLDRVRSQAHSPYRGELRVFEGSCGAALVEGHRHIAR
                     RRASARDTGDRRVAHA"
     gene            3965..4870
                     /locus_tag="VF516_24345"
     CDS             3965..4870
                     /locus_tag="VF516_24345"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8110894.1"
                     /translation="MLKAIDTLRGAERLRAIRAIVALRTPDARPRLIALLGEPPGTLR
                     WLALLGLRKLGGDPASFLAIQRAYATALPEEQGVFLRTASACATTESDVAWILERAEL
                     DHVESGLLVRLRWVAHLPIAPIWLDRALDDVRDLPFGETRHACKATFSIAGIPIAETH
                     FQITIGPRTTERREVATRTRTFQSAFASYAHKDRKRVLARIQGMQKAAPFVDIFFDVK
                     SLRSGDHWEDLIRNEIKARDVLLLFWSRAAQKSRWVKTEWRIALADKGVDGINPVPLE
                     RPSKALKPPRELKALHFDEWTLAIR"
     gene            complement(4918..6711)
                     /locus_tag="VF516_24350"
     CDS             complement(4918..6711)
                     /locus_tag="VF516_24350"
                     /inference="COORDINATES: protein motif:HMM:NF006129.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HEX8110895.1"
                     /translation="MSETVGDFVMQRLYGWGVRTVYGYPGDGINGFLGAFERASDKLR
                     FVQVRHEELAAFMATAHAKFSGQVGVCMATSGPGAIHLLNGLYDAAKDSQPVVAIVGQ
                     QKRISLGADYQQEVDLQRLFGDVAHQFCETCMHPAQARTLIDRAFRIALGTRSVTCVV
                     FPNDVQEEDAVMSAPREHGAVYTGIGFTEGRIIPHDRDLDRAAKLLNDASRVAILVGA
                     GALGASREVAEVADRLGAGVAKALLGKAVLPDDLPYVTGAIGLLGTRPSDLMMQNCDA
                     LLMIGTSFPYSEWLPNEGQARAVQIDLFHRKLGIRYPVDVLLHGDAREALQALLPRLE
                     RKTDRAWRAGIEAAVAEWWQVLEARAHVAADPINPQYVFWTLSPRLPDRCILTADSGS
                     STNWWARDLKIRAGMMASLSGNLATMGPGMPYALAAKFVHPDRPVIATVGDGAMQMLG
                     NNVLIDVAKYWQEWQDPRLIVLVLNNGDLNQVTWEQRVMAGDPKFPDSQQLPAFSFAE
                     YARMLGLEAIEMRTPGDVGPGWDAALAACRPVVVEAFTDPEVPPLPPHIEVRQAKKLM
                     EALIKGDPRRWRVIKQSAKELWAGLVAGLVT"
     gene            complement(6708..>6919)
                     /locus_tag="VF516_24355"
     CDS             complement(6708..>6919)
                     /locus_tag="VF516_24355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110896.1"
                     /translation="RRTTGGAIASGAALGVGLWVVGDELAVPLLGLADKPTAYHPSQH
                     AQGLVEHLAYGVAAATTARALGGVP"
BASE COUNT         1040 a         2504 c         2345 g         1030 t
ORIGIN      
        1 tcccagatcc gcttgaactc gcggaacgcc tcgaccagct ccggcccgaa ctgcttgacc
       61 agcagatcgc cgcgcgactg gccgtcgccg tgctcgccgg agatcgagcc gccgtagcgc
      121 acgcacaggt cggcggcggc gtcggtgaat cgccggtagg tggcgatgcc ctcgggggtc
      181 gacagctcga agtcgatccg gcagtggatg cagccctgtc cgaaatgccc gtacagcgcg
      241 gcctccaggc cgtggtgctg catcagctgg cgcagctcgc gcaggtaggg accgacgcgg
      301 tcgggcgcca ccgcggagtc ctcccagccg ggccagaagt cgcggtgccc cgggatgaac
      361 gccgtcgcgc ccaggccggc ctcgcggacc tcccagacct tgtgcgcctc gtccatccga
      421 tcgaacagct tgatgctcgg cgcgttcggc tcgcgccgga ccgcctcgat catcccgcgc
      481 gcccgcgcca gggcctccgc ctgggtgtcc gcgccgaact cgaccagcag ccagcccttg
      541 ccgtcgggca gaaggccgat gttctcggcg tgtgtcccgg tcgcccgcat gtcgtcgacc
      601 aggcgctggt cgagcccctc caggccgacc ggccggaacc cgcggatctg cgggatgtga
      661 tcgccggcct cgtagacgtc gcggtagccg agcacgcaca gcacgcgctc ggcccgcgcg
      721 gggtacaccg cgagcgtcgc ctcgaggacg gtcacgcagg tcgcctcgct gccgaccagc
      781 gcgcgggcga cgtggaagcc gcgctcgggc aagagctcgt cgaggttgta gcccgagacc
      841 cgccgcggga tcggcgcgaa ccgggtgcgg agcagctccg cgtagcggtc gcggagccgt
      901 gcgagcgcgg cgtagatctc gccggcgcgc cccccggcgg cgatccgccg ggcgagctcg
      961 tcctcggacg tggcgcccac gcgcatccgc aggccatcgt aggtcacgat ctcgagcgac
     1021 tcgacgtgat ccgaggtccg cgcacccggg ccgtacagct cgcccatcac cgagtggatc
     1081 ccgcacgagt tgttccccac catgccgccg agcgtgcagt ggttgtgggt cgcgggatcg
     1141 ggaccgtagg tcaggccgtg cttgccgagc gcggcgcgca gatcgtcgag cacacagccg
     1201 ggctggaccc gcgcccgcca gcgcccggtg tcgacctcga gcacctcgtt catgtacttg
     1261 ctgaagtcga agaccaccgc gcggttgacg gtctggccgg cgaggctggt gccgccgccg
     1321 cgcgacacca ccggcacgcc gtggctgcga caggttcgca gcgcgtgctc gacgtcctcg
     1381 gcgttgcgcg ggatcacgac gccgatcggg acctggcgat agttggacgc gtcgtgggcg
     1441 tagatcgacc gcgatcccgc gtcgaaccgg acctctccgg cgatctcgcg cgcgaggtcg
     1501 gcctcgagct cgctcgcctg cgcgccgcga ccatcggaca ccacgagtct gtcactgcgg
     1561 atcatcgtat gcgctccggc tgcatgcctc gatcgcgatg cagcatgcat tccgccgcgg
     1621 tgcagcatgc gctcgccacg cgccagcgcc gccgcgcgca ccggccgagg tatccggcgt
     1681 gcttgtccct cgatcgagcc ttgcacctgg agaaccacga tgccgatcga tccaccgacg
     1741 actccaccga cgtcccctgc cccaccggcg cccccggcgc gccctgcccg cgtcgatccc
     1801 gagatctggc cctacggctg gccgcgatgg atcccgatcg cgctcggcgc gtggctgttc
     1861 ctctccgcat tcctctggcc gcacggcacc ggcgcagcga tcaacagctg gatcatcgga
     1921 ttactgatcg tgatcgccag cctgatcgcg ctgcgcatgc cgtggatgcg ctgggtcgtc
     1981 accgcgctcg cggtgtggct gttcctgtcg acgctggcga tcgccgcgac ccgcgcgacg
     2041 ctgtggaaca acctgatcgt cgcgatcctc gtgttcctgg catcgctggt gcccgagggg
     2101 ctggacccgt tgctgtcccg cccgcgacgc ggcgcccgcg gcgcggtcag gtgaccagcc
     2161 cggcaggtca cggctcggac aacagctcgg cgaccgacgt acactcgatc gctcgggtga
     2221 tccagcgtcc gagccgcgcg ggatcccgct cggccaggat ccggtcgcgc tccaccgggc
     2281 tcgtcggaac gccgcgagcc gacagcatgg cgacgatcgc ttctgccagg ccttccaccc
     2341 tcccctcggc ccggatcgcc tcgatcaccg ggttgtgctt gacaaccagc gcacgcgcga
     2401 ccgcgtcatc ggtcctggca ctgtggatca gcgtctcgat cggcagcggg acggcgaacg
     2461 ccgggtcggt gatgtgcccc gcggcgtcga gcatgctcca ggtgtcgagc gccgtcgacc
     2521 actccagcgc gcgcgaccgc tcgacgtcga tcgcaaacac gcggcgcacg ccgcgcgcga
     2581 ccagcttggc cgccttgcgg ccggcgtctc ccagtgactg cgtgctgacc acctcgaagg
     2641 cgagctgttc cagcgggcgc cggccggtca cggggtcgcg ggcggcagga tagacgctca
     2701 cgtcgggagc aaggtcgtcg cgtctcgaga tgcgggtcaa cagctcgcac gccgcctgga
     2761 actcggcgcc ggtatgggcc tcgatcagcg ggcagagctg aaactgccgc tccgcgtggg
     2821 gagcatccgc ggggggcacg tgaactagct ccccgtcgag catctcgtgg cgcgtccccg
     2881 gctcgacgag ccggtcatcg agatcgggca ggccggagcc cgcgccagca agatcaagca
     2941 gcatgcccaa ggatacctcc acgaatgcct ccatggggca gcttggcccc gggttcgaac
     3001 ccacgacgag atcatgcccg gcccggcagc aagccccggg ccgccgccaa cctcgcgaga
     3061 tcaccctcgg cgctgtccga ccaacgtccg gatctcggac gatcgtccga gatccggccg
     3121 gacgatggac gatggacgat ggacgatgcc aggtcgctcg acgggtcagc cgcgctggac
     3181 cgcgtccgac gagggccgga cagcagcgcc ggcaccggcc ggatccgact gacgcgcgtc
     3241 cccggctgct gccgctgatt gccgcagcac ggccgatgga tcgtccgccg ccgggcgata
     3301 atggtcgcgt gacgatcgcg tacaccgatg atgaagtggt gaccctcctg cggttcgcat
     3361 tccgaaagga tccgcatcac ctgggggcaa gcgcgctgct gcgcgcgatc tgggtcaacg
     3421 acgacagcct cgctgttgac caggcaaccg cttgtggcca gagcatgggc cgtccacata
     3481 gtagcgttcg attcggcgct tgcaggtgag cccatgcgca agccttgtga tccacaggca
     3541 ctcttggcgc gctgccggaa gcccggctcc aactcctacg acgacgcgcg cctcgataac
     3601 gccgcgcgac aggtggccga cgcgatcgaa cgcggcgctg acgagcactg gaaggcgcct
     3661 gccatggctg cggcgtaccg gatcctcgag aagtcgtcgt ggcctcgttc gccgcagatc
     3721 gaagctgccg cgcgcaggct cctcagccgt gccccgctca aggttgtcgt cggagcacta
     3781 cacctggaga tcctccgtgc gaagactcgc cagatcgcgc tcgaccgtgc tcgccgactc
     3841 tccaccgaca tcgtcgccga tgcagaccgg tacgtgagta actggctcga ccgagtgcga
     3901 tcgcaggcgc attcgccgta tcggggagag ctccgcgtgt tcgagggaag ctgcggcgcc
     3961 gcacttgttg aaggccatcg acacattgcg aggcgcagag cgtctgcgcg cgatacgggc
     4021 gatcgtcgcg ttgcgcacgc ctgatgccag accgcggctg atcgcgctgc tcggcgagcc
     4081 acctggaacg ctgcgctggc tcgcactgct cggccttcgc aagcttggcg gcgatccagc
     4141 gtcgttcctt gcgattcagc gcgcttatgc gaccgcgctc cccgaggagc aaggcgtgtt
     4201 cctccggacg gcgagcgcct gcgcgacgac ggagagcgac gtcgcctgga tcctcgagcg
     4261 cgccgagctc gaccacgtcg agtcagggct cctcgtccga ctcagatggg tcgcacacct
     4321 gccgatcgca ccgatctggc tggatcgtgc gctcgatgac gtgcgcgacc ttccttttgg
     4381 cgagacgaga cacgcgtgca aggcgacatt cagcatcgcc ggcattccga tcgcagaaac
     4441 gcatttccag atcacgatcg ggccgcgcac cacggagcgg cgcgaggtcg cgacccgtac
     4501 ccggacgttc cagtccgcgt ttgcgtcgta cgcccacaaa gatcgcaaac gcgtgctcgc
     4561 gcgcatccag ggcatgcaga aggcggcacc gttcgtcgac atcttcttcg acgtcaagtc
     4621 gctgcgctcc ggcgatcact gggaagacct gatccgcaac gagatcaagg cccgcgacgt
     4681 cttgctcctg ttctggtcgc gcgcggccca aaagtcacgc tgggtgaaga ccgagtggcg
     4741 gatcgcgctt gccgacaaag gggtcgacgg catcaatccc gttccgctcg agaggcccag
     4801 caaggctctg aaaccaccgc gcgagctgaa agcgctccac ttcgatgagt ggacgctggc
     4861 gatccgttga ccttccacat cgcccggact gctcggctcg gccgatccgc ggcgcggtca
     4921 ggtgaccagc ccggcgacca gcccggccca cagctccttg gccgactgct tgatcacgcg
     4981 ccagcgccgc gggtcgccct tgatcagcgc ctccatcagc ttcttggcct gacggacctc
     5041 gatgtgcggc ggcagcggcg ggacctcggg atcggtgaac gcctcgacga ccaccggccg
     5101 gcacgccgcc agcgccgcgt cccatcccgg cccgacgtcg ccgggcgtgc gcatctcgat
     5161 cgcctcgagg ccgagcatgc gcgcgtactc ggcgaacgag aacgccggca gctgctgcga
     5221 gtcggggaac ttgggatcgc ccgccatcac ccgctgctcc caggtcacct ggttgaggtc
     5281 gccgttgttg agcacgagca cgatgagccg cgggtcctgc cactcctgcc agtacttcgc
     5341 gacgtcgatc agcacgttgt tgccgagcat ctgcatcgcg ccgtcgccga ccgtcgcgat
     5401 caccgggcga tcggggtgga cgaacttcgc ggccagcgcg tacggcatgc ccggccccat
     5461 cgtcgcgagg ttgcccgaca gcgaggccat catcccggcg cggatcttga gatcgcgcgc
     5521 ccaccagttg gtcgacgagc ccgagtcggc cgtgaggatg cagcggtcgg gcagccgcgg
     5581 cgacagcgtc cagaacacgt actgcgggtt gatcggatcg gcggcgacgt gggcgcgcgc
     5641 ctcgagcacc tgccaccact cggcgaccgc ggcctcgatg cccgcgcgcc acgcccgatc
     5701 ggtcttgcgc tcgagccgcg gcagcagcgc ctggagcgcc tcgcgcgcgt cgccgtgcaa
     5761 caggacgtcg accggatagc ggatgccgag ctttcggtgg aacagatcga tctggaccgc
     5821 ccgggcctgg ccctcgttgg gcagccactc cgagtacggg aagctggtgc cgatcatgag
     5881 cagcgcgtcg cagttctgca tcatcagatc gctcggcctg gtgccgagca ggccgatcgc
     5941 gccggtcacg tacggcagat cgtcgggcag caccgccttg ccgagcagcg ccttggcgac
     6001 gccggcgccc agccgatcgg cgacctcggc gacctcgcgc gatgcgccga gcgcaccggc
     6061 gccgaccagg atcgcgaccc gcgacgcgtc gttgagcagc ttcgccgcgc gatcgagatc
     6121 gcgatcgtgc gggatgatcc ggccctcggt gaagccgatc ccggtgtaca cggcgccgtg
     6181 ctcgcgcggc gcgctcatca ccgcgtcctc ctcctggacg tcgttgggga acacgacgca
     6241 ggtcacgctg cgcgtgccga gcgcgatgcg gaacgcgcga tcgatcagcg tgcgggcctg
     6301 cgcggggtgc atgcaggtct cgcagaactg atgggcgacg tcgccgaaca gccgctggag
     6361 gtcgacctcc tgctggtaat cggcgcccag cgagatccgc ttctgctggc cgacgatcgc
     6421 gaccaccggc tggctgtcct tcgccgcgtc gtacaggccg ttgagcaggt ggatcgcgcc
     6481 cgggcccgag gtcgccatgc acacgccgac ctggccggag aacttggcgt gcgcggtcgc
     6541 catgaacgcc gcgagctcct cgtggcggac ctgcacgaag cgcagcttgt ccgacgcgcg
     6601 ctcgaacgcg ccgaggaagc cgttgatgcc gtcgcccggg tagccgtaga ccgtgcgcac
     6661 gccccagccg tacagccgct gcatcacgaa atcaccgacg gtctcgctca tggcacacct
     6721 ccgagcgcgc gggcggtggt cgccgccgcc acgccatacg cgaggtgctc gaccaggccc
     6781 tgggcgtgct ggctcgggtg gtacgcggtc ggcttgtcgg cgaggccgag cagcggcacc
     6841 gcgagctcgt cgccgacgac ccacagcccg acgccgagcg ccgcgcccga ggcgatcgcg
     6901 ccgccggtcg tgcgccggc
//
LOCUS       DASVOR010001273         1951 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_158849,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001273 DASVOR010000000
VERSION     DASVOR010001273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1951
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158849"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            239..967
                     /locus_tag="VF516_24360"
     CDS             239..967
                     /locus_tag="VF516_24360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110897.1"
                     /translation="MRFRACSILVALLGPAAACAVADEPAADPAAADTEIPYTTRGFT
                     ETYSWGTRDIGHWTLTTNDLRSRVIEQTGGNPGGYLYGEVASPIPTWSTASRRVDPEL
                     GRDTIFVGNYSANRVRRISADLCVYQAGSWSASRTVTLQLVRWDVPNATVALEATYSL
                     PDIPAVPNGWRRYEFAVDAQSATIPPGWELQRGDGTPATDADWALLMQQIDVVGFGFW
                     KMGTMYPALGLWELGIDNLHIGTW"
     gene            1319..1882
                     /locus_tag="VF516_24365"
     CDS             1319..1882
                     /locus_tag="VF516_24365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110898.1"
                     /translation="MTAKRDLKRRVRERQGRTGESYMTALRHVLDQRPNAVPVVEMIE
                     LSEVADAMGIQCRFRMSPALADRIDAVGALRRLRDALIATERDPAFDLFRRVVLHGES
                     PVLRPTIGGIDAGRRFIARVRAGIGGISEHGRLLALMVDGRRGPEMVVFMLWLSPVAH
                     PRYQPSLILAVADGTIDGPGTLSITWR"
BASE COUNT          271 a          693 c          693 g          294 t
ORIGIN      
        1 ctcccatcga ccgagcgagg tacggcaccc gcgtcccccg gcgggcgcgt catcgccggc
       61 tgcgccggtc ggtcgagcgc agcgcgcgaa caggctgcgc ccgcgcccgc acgctcggca
      121 tcgcttgcgc acacggagcc gtgctcgatc gatcggcact tgcacgccgg tggtcgcccg
      181 cgggtcgctc acgcgcggca gtggtcttgc acgaagggtc gctcgaaagg agtcgatcat
      241 gcgattccgt gcttgttcca ttctcgtggc gctgctcgga cccgccgcgg cgtgcgcggt
      301 cgccgacgag cccgccgccg atcctgcggc cgccgatacc gagatcccgt atacgacccg
      361 cggattcacg gagacctaca gctgggggac ccgcgatatc ggccactgga cgctgaccac
      421 gaatgatctg cgctcccggg tcatcgagca gaccggcggc aaccccggcg gttatctcta
      481 tggcgaggtc gccagcccga tcccgacctg gtcgaccgcg tcgcggcgag tcgacccgga
      541 gctcgggcga gacacgatct tcgtcggcaa ttactccgcg aatcgcgtcc gccggatcag
      601 cgcagatctg tgcgtgtacc aggccgggtc atggtcggcg agccgcacgg tgaccctgca
      661 gctcgtgcgc tgggatgtcc cgaacgccac cgtcgcgctc gaggcgacgt acagcctgcc
      721 ggacatcccc gcggtgccca acggctggcg gcgctacgag ttcgcggtcg atgcacagtc
      781 ggcgacgatc ccgccgggat gggagctcca gcgcggcgac gggacgcccg ccaccgacgc
      841 cgactgggcg ctcctgatgc agcagatcga cgtcgtcggc ttcggcttct ggaagatggg
      901 caccatgtat cccgcgctgg ggctgtggga gctcggcatc gacaaccttc acatcggcac
      961 gtggtgagcc ggcgcggtgt cagcggcgag cgtcgcggcg gtgctgcacg gtgatccgga
     1021 tcctacgcgc ccgttcttcc atcgccgggg cttgccgagc agccagtcgt gcgtgccgga
     1081 ccgtgcgccg gactcacggt tgacatcgtg ccgcggtgcg gcgatcctgt gaggacataa
     1141 acgcgggctg ccacgcgtac cctcacgcga tcggtccatg cccgatcgca cccgggggca
     1201 aaggggagtc ctctcctcgg cggcactgga tctcatcgtc tacgtcgtct gcgttcggca
     1261 gcgtccaccg gcgctgcggg ggagcatgga gccaccgcgg cgcatcggaa ggatgtcgat
     1321 gaccgcaaaa cgagatctca agcgccgcgt ccgggagcgc cagggccgga ccggcgagtc
     1381 gtacatgacc gcgctgcgcc atgtcctcga ccagcggccc aacgcggtcc cggtggtcga
     1441 gatgatcgag ctgtccgagg tcgccgacgc gatggggatc cagtgccggt tccggatgtc
     1501 gccggcgctc gccgaccgga tcgacgccgt gggcgcgctg cgccggctgc gcgacgcgct
     1561 gatcgcgacc gagcgcgatc cggcgttcga cctgttccgg cgcgtggtgc tgcacggcga
     1621 gtcgccggtc ctccgcccga cgatcggcgg catcgacgcc ggccggcggt tcatcgcgcg
     1681 ggtccgcgcc gggatcggcg ggatcagcga gcacggccgg ctgctcgcgc tgatggtcga
     1741 cggccggcgc ggccccgaga tggtcgtgtt catgctgtgg ctgtcgccgg tcgcgcaccc
     1801 ccggtaccag ccatcgctga tcctggcggt agcggacggc acgatcgacg ggccgggcac
     1861 cctgtcaatc acctggcgct agccaccggc aagctcgggc cggtgccggc gctcgcggtc
     1921 gcgagccaca tgctggtcgc cgcgcgcgac c
//
LOCUS       DASVOR010001274         2610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_129781,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001274 DASVOR010000000
VERSION     DASVOR010001274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2610
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129781"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..184)
                     /locus_tag="VF516_24370"
     CDS             complement(<1..184)
                     /locus_tag="VF516_24370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110899.1"
                     /translation="MLRIKLRYDDPETMVLRFAANVGKSGLFLPTRSMQPIGAEVKFE
                     LRLADDTPALVGLGRVK"
     gene            627..983
                     /locus_tag="VF516_24375"
     CDS             627..983
                     /locus_tag="VF516_24375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110900.1"
                     /translation="MTPLWHDLRDALRTMAKAPGLTAVLVITLALGIGATTTIFSVVN
                     SASAAAAALPPRLPLPIAVPAPAAVPALAAVPAPAAVPAVDGRRSSTVSWLAAVATGA
                     APRVRCAYPGNVRELA"
     gene            1071..>2610
                     /locus_tag="VF516_24380"
     CDS             1071..>2610
                     /locus_tag="VF516_24380"
                     /inference="COORDINATES: protein motif:HMM:NF013713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110901.1"
                     /translation="MRADNKLLAGVFLACFANLLLEVMITRLFSATMFYHFTFLAVAL
                     ALFGVAASGVYVFVAGDRLAEDVRGQLARASRLFAAGAVVALTYAMANPIDVIIVTGT
                     NQVPQFTARQVWQLIFLVGVTAVPFYSGGLVVSLALTWLRHDTGRIYAYDLAGAAAEA
                     LVTGVVVSLVGGPTAVLIAAAVALVAAALFDGRRWWLVACGAAVVACNLALPVIRVPT
                     IKGVKQEATRFEAWNAFSRVTVDDTSTIKIDASAATHIESLRALTPGAYRTEISALAH
                     AMFDPPAARVAIIGPGGGRDVLHALSAGATDVTGIEVNPIIADTIMRDVYRTASGGLY
                     DDPRVHIVVDDGRSFIRRSAERYDVVQASLVDTWAATAAGAFALTENALYTVEAFRDY
                     FAHTTDRGAVTMTRWHSGAGGETARLLILAAAALEAGGVAPGEVRQHIIYAVSPRAGL
                     GTMIAKRLPITPDELARVEAAAAAAHQPRFHRYSPTAGLPELREAIAAKTARDSGYQV
                     LPGQV"
BASE COUNT          326 a         1014 c          895 g          375 t
ORIGIN      
        1 ccttgacccg gccaagcccg acgagcgccg gggtgtcgtc ggccagccgg agctcgaact
       61 tgacctcggc gccgatcggc tgcatcgacc gcgtcggcag gaacagcccc gactttccca
      121 cgttggccgc gaaccgcagg accatcgtct ccggatcgtc gtagcggagc ttgatgcgga
      181 gcacgatcgg cgccgccggt cgtccaccac tgggatcgtc aggagccatg gatgggcccc
      241 ccggcttggc ccgtggccag tatcccgctc ccggcgagat cccgtcaaac gaccggcgct
      301 ccgagagcaa cgcgcagccg gggctccgat cgccagcgcc ccggccccgg tcggcttctg
      361 acgccggatc tcgcggtcga gcggcgctgc agcgcactgc ggcggcggcc cgtgccaccg
      421 ggtcggaacc ccgggtacca cccgccgcga cgaacgagcc tccagctcgc cgagcgactc
      481 cgcggacggg ccgaggcgcg tcccgcggag cgaagcccag gtgtccggac atgggatcgg
      541 cgatcccatg tccggacagc ggccggccga aacgccacgg aagtccgcgg ggttgagctg
      601 gcacgccggg tgctgcaccg gcgagcgtga cgccgctgtg gcacgacctt cgcgatgcgc
      661 tgcgcacgat ggcgaaggcg cccgggctga ccgccgtgct ggtcatcacc ctggcgctgg
      721 ggatcggggc gaccaccacg atcttcagcg tggtcaactc cgcttcggct gccgctgccg
      781 cgctgccgcc tcggctcccg ctcccgatag cggttccggc accggcagcg gttccggcac
      841 tggcagcggt tccggcaccg gcagcggttc cggccgtcga cggtcgtcgc tcgtcgaccg
      901 tgtcgtggct cgccgcagtc gctaccggcg cagcaccgcg cgtccgctgc gcgtaccctg
      961 gcaacgtccg cgagctcgcg tgatccagcg cgccggcgtg ctcgccacgg cgacgagatc
     1021 tccccgtcgg tcctgttgcc gcggcggcgg cggtgtagca tcccgcggcg atgcgcgccg
     1081 ataacaagct gctcgccggc gtgttcctgg cgtgcttcgc caacctgctg ctcgaggtga
     1141 tgatcacccg gctgttctcg gcgacgatgt tctatcactt caccttcctc gcggtcgcgc
     1201 tcgcgctgtt cggcgtcgcg gcgagcgggg tctacgtgtt cgtcgccggc gaccggctcg
     1261 ccgaggacgt ccgcggccag ctcgcgcgcg cgtcgcggct gttcgccgcc ggcgccgtgg
     1321 tcgcgctgac gtacgcgatg gccaacccga tcgacgtcat catcgtcacc ggcaccaacc
     1381 aggtgcccca gttcaccgcg cgccaggtct ggcagctgat cttcctggtc ggcgtcaccg
     1441 ccgtgccgtt ctattccggc ggtctggtcg tgtcgctcgc gctgacctgg ctgcgccacg
     1501 acaccggccg gatctacgcc tacgacctcg cgggcgcggc ggccgaggcg ctggtcaccg
     1561 gggtcgtcgt gtcgctggtc ggcggaccga ccgcggtgct gatcgccgcc gcggtcgcgc
     1621 tggtcgccgc cgcgctgttc gacggccggc gctggtggct ggtcgcgtgc ggcgccgccg
     1681 tcgtcgcgtg taacctcgcc ctcccggtca tccgcgtgcc caccatcaag ggcgtcaagc
     1741 aggaggcgac ccggttcgag gcgtggaacg cgttctcgcg ggtcacggtc gacgacacct
     1801 ccacgatcaa gatcgacgcc agcgcggcga cccacatcga gagcctccgg gcgctcaccc
     1861 ccggcgcgta ccggaccgag atctccgcgc tcgcccacgc gatgttcgat ccgccggccg
     1921 cccgggtcgc gatcatcggc cccggcggcg gccgcgacgt gctccacgcg ctgtcggccg
     1981 gcgcgaccga cgtcaccggg atcgaggtca acccgatcat cgccgacacc atcatgcgcg
     2041 acgtctaccg cacggcgtcc ggcgggctgt acgacgaccc ccgcgtgcac atcgtggtcg
     2101 acgacggccg cagcttcatc cggcgctccg ccgagcgcta cgacgtcgtc caggcctcgc
     2161 tggtcgacac ctgggccgcc acggcggccg gcgcgttcgc gctgaccgag aacgcgctct
     2221 acaccgtcga ggcgtttcgc gactacttcg cccacaccac cgatcgcggc gcggtcacca
     2281 tgacccgctg gcacagcggc gccggcggcg agaccgcgcg gctcttgatc ctcgccgcgg
     2341 cggcgctcga ggccggcggc gtcgcgcccg gcgaggtccg ccagcacatc atctatgccg
     2401 tgtcgccgcg cgccgggctc gggacgatga tcgccaagcg gctgccgatc acgcccgacg
     2461 agctcgcgcg ggtcgaggcc gcggcggccg ccgcccacca gccgcgcttc caccgctaca
     2521 gcccgaccgc cggcctaccc gagctgcgcg aggcgatcgc cgccaagacc gcccgcgact
     2581 ccggctacca ggtccttccc ggccaggtgc
//
LOCUS       DASVOR010001275         8929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_275363,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001275 DASVOR010000000
VERSION     DASVOR010001275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8929
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275363"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..684
                     /locus_tag="VF516_24385"
     CDS             <1..684
                     /locus_tag="VF516_24385"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8110902.1"
                     /translation="LDRVPLDEAAVAERTAHYAALHDQRCRALLAAEHPDGDAITPAY
                     LTACVRRLIDDRTIVVNEGVSNYETITNHLRLTRPGSMLTSGGSSLGWSGGAAIGVAL
                     ARRDHTVICLTGDGSYLFSQPSVVHWMARRYDTPFLQIVYNNGGWKAPKLGLLALHPS
                     GHGARADDLRLGFAPEPDHAGIAAAAGGAFAGVVTRASELDAALDAAMTAVRRERRCA
                     VLDVKLPPL"
     gene            complement(716..1645)
                     /locus_tag="VF516_24390"
     CDS             complement(716..1645)
                     /locus_tag="VF516_24390"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX8110903.1"
                     /translation="MISFPRYAWKDGEIIDWKDCTLHVRSQGCFFGTNVFEGLRGYWN
                     PRTEEIYVFRVAEHMTRLGLSMKVMRLKVSHSFPDIVAGMTKLLRANQFREHVQINIV
                     AYFGLNPTPDPLYPSETTGVHVTAIPFPRSASVETGIHAAISSWRRISDDVMPPRVKV
                     GSNYQNSRLAQSEVRLNGYDMAILLNPQGTVAEGPGACVFAVRDGELHTPDLASGILE
                     SITRATLIELARKELGLTVVERDVNRTELYAADEVFACGSIAEITPIVSVDRLQVGNG
                     SVGPVTRQLQQLYFKAVLGELPGYEHWVTPALR"
     gene            complement(1689..2342)
                     /locus_tag="VF516_24395"
     CDS             complement(1689..2342)
                     /locus_tag="VF516_24395"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HEX8110904.1"
                     /translation="MTGDYRMLGSLCFTGFLLGWSVAWPPGPVNAEIVRRGLSRGFWA
                     AYGMALGGCTGDAVWAMLVMFGSGAVLGSETASSVLRTASTGLLIFLSAHYLYGAWRS
                     WARLRAGTGVSHERPPQFDRIGVGFVLGLGLTLTSPWSIAFWLAVMGRSEVSAHGLTG
                     ALMFALSVIAGAATWALILSLTVVVLRSRFNSPRWDVITKAGTGLLLLYFAVRALTA"
     gene            complement(2355..3455)
                     /gene="hppD"
                     /locus_tag="VF516_24400"
     CDS             complement(2355..3455)
                     /gene="hppD"
                     /locus_tag="VF516_24400"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEX8110905.1"
                     /translation="MSRVEAIDHLGFIVGDAAMTARFFGNELGFRAIAERADQSEMQS
                     LVMAQRDIRLVFSSPLSPTSLHSDHLRLHGSGVRDIAFATPDVRAAYRTFIERGARSI
                     SEPRPYRANGGVLWHATVGSFCDSLVHSLIQRDGHTQALWPGHRDPDGWTPLLDLALD
                     AIDHVAICTDWDQLDEVTGFFARVFDFEVSHEEDVATEHTGMRSRVMQSANHAVRLVF
                     MEPVHGARESQIAEYLRHYGGPGVQHVAFHTGDIIAASHKLRDRHIPFLRIPATYYDQ
                     LEARIGRVPYQLDQLCESNVLVDREEDGLLLQAFTRPIYARPTFFCELIQRRGALGFG
                     GGNIAALFRAVEADRLRAAGHAEPSRPAARRG"
     gene            complement(3466..5289)
                     /locus_tag="VF516_24405"
     CDS             complement(3466..5289)
                     /locus_tag="VF516_24405"
                     /inference="COORDINATES: protein motif:HMM:NF006002.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8110906.1"
                     /translation="MSGFDMGESPLNTMTAGSREGRHSLPAIAIVGAGPVGLAAALRL
                     ASFGIPCALLEANPALLKRGSKALAIQHGVLRILDLMGCADELVAEGTPWSISRTFVR
                     DQELFHTHFTTNDALPPFLNIPQFRTEQILLEHVRRTGLVTELWEHAVVGIEQDDSRV
                     VIEADTLAGRRSYECQYLIGCDGLRSAVRSVLGIEWLGYTHADRFLIVDIRTDVNFPR
                     ERRFYFDARSNPGRQIVVHAQPGKMWRIDWQLPPDADVEAEQRSGKLDARIRALIGDA
                     PYEIDWLSTYRFHQRRAARIRVGRVMLAGDAAHAMPPFGARGMNSGIQDVDNLAWKLK
                     LVTEGIASPALLESYEIERQVAAQENLAVTESTIRFMVPPTRWHRLWRDSILAMGRRY
                     PPLSALVNSGQMSRPNRYRSSPIITRDPERLERWPLALRRPRDNEPGSLLVDASCRVD
                     GTVTRLRKLASQQFVGLYFCADVGLAAEFARGYVAAAGGTAVPFRLYLVPPVGQRVDI
                     PAMPEIGVAPVGRIDDFTGALNGIYSDRHPTFYLIRPDGYIAARERDFPPHEVAALIG
                     RSAGLAAGAVGDGGAGGKRSRDSASPSRWQSARTIFQSRHG"
     gene            complement(5593..6600)
                     /locus_tag="VF516_24410"
     CDS             complement(5593..6600)
                     /locus_tag="VF516_24410"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX8110907.1"
                     /translation="MRAYEVQPREGFDALTLVDRAPPPLGPTDVRVRVRAVSLNYRDL
                     TIARGAAKRKAPVIPASDGAGEVVEVGAAVTRHKVGDRVAASFFPRWIDGAMSEQAHA
                     AALGGSVDGMLAEEVVLPETAWVRLPARLSFEAAATLPCAGVTAYHALFEATQLRPGD
                     TVLVQGTGGVSIFGLQLARAAGARVIVTSSSAAKRERALAMGADHVIDYKAEPKWGDA
                     ARAWTGGRGADVVIEVGGPGTFDQSVAALRYGGTMSLLGVLTGTKGEIQTYGIFHKGL
                     RVAGIYVGSVAMFEGLVRALEARQIEPVIDRTFGFAEARAAYEYLASAQHFGKVVIRV
                     A"
     gene            7014..8723
                     /locus_tag="VF516_24415"
     CDS             7014..8723
                     /locus_tag="VF516_24415"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8110908.1"
                     /translation="MRRALFGLVAVLAIAAFVRLAPADDRDDGDDHGNDQLQLVEATI
                     PQLDQALRSHLITSRQLVEMYQARIAAYDKVGPTFNAFIHLNPNAAAEGAARDREHGH
                     EGDRSPMFGIPILLKDNVDTSDQPTTAGSIALTGSVPPQDAFITKRLRDAGAVILGKA
                     TLTEFANFIAIGMPSGYSSLGGYGLNPFDPRPLPGGDGRPVLTPGGSSSGPGIAVSAN
                     LVTIAIGTETSGSILSPSTANGNAGIKPTLGLVSRTGILPITANQDTAGPIARTVTDA
                     AILLGVIAGFDRDDPATLPCLVPGNCFRDYTRFLDKHALQGARIAVPHVPYWNGFNAD
                     QTAIMNEVIGELRAAGAFVDDPHELPNQADISAFGICTSAPAPLTCSTVLLYGQKHDL
                     NAYLTRRPNAPVHTIDDIIAFNNANPIAGLKYGQALFLAAATLDTSPGSADTLRVNAD
                     RAKDLQLTQGGIDAVLNGPDGIAGTADDFDAVLFPQNRGAAAPAKAGYPSVAVPTRFQ
                     VPAGPIVNSFPFGVAFTGRAFSEPRLIALAFAYEQATHHRVAPASAPPLPSDTVTRHH
                     GDD"
BASE COUNT         1473 a         3205 c         2974 g         1277 t
ORIGIN      
        1 ctcgaccgcg tgccgctcga cgaggccgcg gtcgccgagc gcaccgccca ttacgccgcg
       61 ctccacgacc agcgctgccg cgccctgctc gccgccgagc accccgacgg cgacgcgatc
      121 acccccgcgt acctcaccgc ctgcgtgcgc cgcctgatcg acgaccgcac gatcgtggtc
      181 aacgagggcg tctccaacta cgagacgatc accaaccacc tgcgcctcac ccggcccggc
      241 agcatgctga ccagcggcgg cagctcgctg ggctggagcg gcggcgccgc gatcggcgtt
      301 gcgctcgcgc gccgcgacca caccgtgatc tgcctgaccg gcgacggctc gtacctgttc
      361 tcgcagccgt cggtggtgca ctggatggcc cgccgctacg acacgccgtt tctccagatc
      421 gtctacaaca acggcggctg gaaggcgccc aagctggggc tgctggcgct gcacccctcg
      481 ggccacggcg cccgggccga cgacctccgc ctcgggttcg cccccgaacc tgatcacgcc
      541 ggcatcgccg cggccgccgg cggcgccttt gccggcgtgg tgacccgcgc gtcggagctc
      601 gacgccgcgc tcgacgccgc catgaccgcc gtccgccgcg agcgccgctg cgctgtcctc
      661 gacgtcaagc tgccgccgct gtgacggcta ccgatcagcg ataccgctcg acgcgctatc
      721 gcagcgccgg ggtcacccag tgttcgtacc ccggcagctc gccgagcacc gccttgaagt
      781 acagctgctg gagctgccgg gtcacggggc cgacgctgcc gttgccgacc tgcaggcgat
      841 ccaccgagac gatgggcgtg atctcggcga tcgatccgca ggcgaagacc tcgtctgccg
      901 catagagctc ggtgcggttg acgtcgcgct cgaccacggt gagtcccagc tccttgcgcg
      961 ccagctcgat caacgtcgcc cgggtgatgc tctcgagtat tcccgacgcg agatccggcg
     1021 tgtgcagctc cccgtcgcgc accgcgaaca cgcacgcgcc cggaccttcc gccaccgtcc
     1081 cctgcgggtt gagcaggatc gccatgtcgt aaccattgag ccggacctcg ctctgggcga
     1141 gccggctgtt ctggtaattg gagccgacct tcacccgggg cggcatgaca tcatcgctga
     1201 tcctgcgcca cgacgagatc gcggcgtgga tgccggtctc gacgctcgcc gacctcggaa
     1261 acgggatcgc ggtcacgtgg acgccggtgg tctccgatgg gtacagcgga tccggcgtgg
     1321 gattcagacc gaagtacgcg acgatgttga tctgcacgtg ctcccggaac tggttggcgc
     1381 gcagcagctt cgtcatgccg gcgacgatgt cggggaacga gtgcgagacc ttcagccgca
     1441 tgaccttcat cgagagcccc agccgggtca tgtgctcggc cacgcgaaac acgtagatct
     1501 cctcggtcct cgggttccag tagccgcgga gcccctcgaa gacgttggtg ccgaagaagc
     1561 acccctggct tcgcacgtgc agcgtgcagt ccttccagtc gatgatctca ccgtctttcc
     1621 aggcgtatcg agggaaactg atcatgggtc atgccatccg gttggaagag tggtctcggc
     1681 gaacgggatc atgcggtgag cgcgcgaacc gcgaagtaga gcagcagcag gccggttccg
     1741 gccttggtga tcacgtccca gcgcggactg ttgaatcgcg agcgcagcac gaccacggtg
     1801 agcgacagga tcagcgccca ggtcgccgca ccggcgatca ccgacagcgc gaacatcaag
     1861 gctccggtga gcccgtgggc cgatacctcc gagcgcccca tcacggccag ccagaacgcg
     1921 atgctccagg ggctggtcag ggtcaggccg aggccgagca cgaacccgac gccgatcctg
     1981 tcgaactgcg gcgggcgctc gtgcgaaacc ccggtgccgg cgcgcaaccg ggcccacgac
     2041 cgccatgcgc cgtacagata gtgagcggag aggaagatca acaggccggt gctcgccgtg
     2101 cgcagcaccg agctcgcggt ctcgctgccg agcacggcgc cggagccgaa catgacgagc
     2161 atggcccaca ccgcgtcccc ggtgcagcct ccgagggcca tgccgtacgc ggcccagaag
     2221 ccgcgcgaca acccgcgccg cacgatctcg gcgttgaccg gccccggcgg ccatgccacc
     2281 gaccagccga gcagaaagcc ggtgaagcac agcgaaccga gcatgcgata gtcgccggtc
     2341 atggtggaac cagattaccc gcgccgcgcg gccggacgcg agggctccgc atgcccggca
     2401 gctcgcagcc ggtcggcctc gacggcgcga aacagcgcgg cgatgttgcc gccgccgaat
     2461 cccagggcac cgcgacgctg gatcagctcg cagaagaacg tcggccgggc atagatcggc
     2521 cgggtgaacg cctgcagcag caacccgtcc tcctcgcgat cgaccaggac gttggactcg
     2581 cacagctgat cgagctgata cgggacccga ccgatgcgag cctcgagttg atcgtagtag
     2641 gtggcgggga tgcggaggaa cgggatgtgc cgatcgcgca gcttgtggct cgccgcgatg
     2701 atgtcgccgg tgtgaaacgc gacgtgttgc acgcccggcc caccataatg ccggaggtac
     2761 tcggcgatct ggctctcgcg cgcgccgtgc accggctcca tgaacacgag ccggaccgcg
     2821 tgattcgcgc tctgcatgac gcgcgacctc atgccggtgt gctcggtcgc gacgtcctcc
     2881 tcgtgcgaga cctcgaagtc gaagacccgc gcgaagaacc cggtgacctc gtcgagctga
     2941 tcccagtcgg tgcagatcgc gacgtggtcg atcgcgtcga gcgccagatc gagcaagggc
     3001 gtccagccgt cggggtcgcg gtggccgggc cacaacgcct gcgtgtggcc gtcgcgctgg
     3061 atcagcgagt gcaccaggct gtcgcagaac gagccgaccg tggcgtgcca cagcactcca
     3121 ccgttcgcgc ggtacgggcg cggctcgctg atcgaccggg cgccgcgctc gatgaacgtg
     3181 cgataggccg cgcggacgtc cggggtggca aacgcgatgt cccggacccc cgagccgtgc
     3241 aaccggaggt ggtcgctgtg aaggctggtc ggcgacaggg gtgacgagaa caccagacgg
     3301 atgtcgcgct gggccatgac cagcgactgc atctcgctct ggtccgcacg ctcggcgatg
     3361 gcgcggaatc cgagctcgtt gccgaagaat cgcgccgtca tcgcggcgtc gccgacgatg
     3421 aacccgaggt gatcgatcgc ttcgacacgt gacatgcaga actcctcagc cgtggcggct
     3481 ctggaagatg gtccgcgcgg attgccagcg cgacgggctg gcgctgtccc gtgatcgctt
     3541 tcccccggct cccccgtctc cgactgcgcc ggcggcgagg cccgccgatc tgccgatcag
     3601 cgcggcgact tcgtgaggag ggaaatcgcg ttctcgtgcg gcgatgtagc cgtccgggcg
     3661 aatcaggtag aacgtggggt ggcggtcgct gtagatgccg ttgagcgccc cggtgaagtc
     3721 atcgatccgg ccgaccgggg ccacgccgat ctcgggcatc gccgggatgt ccacccgctg
     3781 gccgaccggc gggacgaggt agagccggaa tggcaccgcc gtgccgccgg cggccgcgac
     3841 gtagccgcgc gcgaactcgg cggccagccc gacgtcggcg cagaagtaca ggccgacgaa
     3901 ctgctggctg gccagcttgc gcaggcgcgt cacggtgcca tcgacccggc agctggcatc
     3961 gaccagcagg ctgcccggct cgttgtcgcg aggccggcgc agcgcgagcg gccagcgctc
     4021 gaggcgctcc ggatcccggg tgatgatcgg agacgagcga tagcgattcg gtcgcgacat
     4081 ctgcccgctg ttgaccagcg cactgagcgg cggatagcgc cggcccatgg cgaggatcga
     4141 gtcgcgccac agccggtgcc agcgggtcgg cggcaccatg aaccggatcg tggactcggt
     4201 caccgcgagg ttctcctgcg ccgcgacctg gcgctcgatc tcgtagctct cgagcagcgc
     4261 cggggacgcg atgccctcgg tgaccagctt gagcttccag gccaggttgt cgacgtcctg
     4321 gatgccgctg ttcatgccac gcgctccgaa cggcggcatc gcgtgcgcgg cgtcgccggc
     4381 gagcatcacg cgaccgacgc ggatccgcgc ggcacggcgc tgatggaagc ggtacgtgct
     4441 gagccagtcg atctcgtacg gcgcgtcccc gatcagcgcg cggatccgcg catcgagctt
     4501 gccggagcgc tgctcggctt cgacgtcggc gtccggcggc aactgccagt cgatccgcca
     4561 catcttcccg ggctgggcat gcacgacgat ctggcggccg gggttcgagc gtgcgtcgaa
     4621 gtagaaccgc cgctcgcgcg ggaagttgac atcggtgcga atatccacga tcaggaaccg
     4681 atccgcgtgc gtgtatccga gccactcgat tccgagcacg ctgcgcaccg cgctccgcaa
     4741 accatcgcag ccgatcaggt actggcactc gtacgaccgc cggcccgcca gggtgtcggc
     4801 ctcgatcacg acccgggagt cgtcctgctc gatgccgacc accgcatgct cccacagctc
     4861 cgtgaccagg ccggtacggc ggacgtgctc gaggaggatc tgctcggtcc ggaactgagg
     4921 gatgtttagg aacggcggga gtgcatcgtt cgtcgtgaag tgagtatgga acagctcctg
     4981 atcgcggacg aaggtgcggg agatcgacca cggcgtgcct tccgccacca gctcatcggc
     5041 acaacccatc agatcgagga ttctcaagac gccatgttga atcgcgagcg ctttcgatcc
     5101 gcgcttcagg agagccggat tggcctccag caatgcgcag gggataccga acgacgccag
     5161 gcgcagcgcc gcggcgaggc cgaccggtcc ggcaccgacg atggcaatgg ctggcaatga
     5221 gtgacgccct tcacgcgaac cggctgtcat ggtattcaac ggcgactccc ccatatcaaa
     5281 accggacatc gctctagcat gtcggtcaat cgcgtcgcaa tgacggatca gagaacttcg
     5341 aatcgtcgcg atccgaatgc atgtgatgca ccacctcggg ccgcgacgag ggcaaggtaa
     5401 atcaagattt taggaatcga tcgcgggatc tgtcgtaggc tagcaaacgt gtgcattacc
     5461 cgacacacgc cggacacacg ccgtacgttg catcacgcaa aagcaatcgc atggtcacac
     5521 gggagctcgg gagcggcgcg atcggcggcc cggcagagcc gagcgatctc gaggcttcga
     5581 tctcggatcg cctcaggcca cgcggatcac gaccttgccg aagtgctggg cgctggcgag
     5641 gtactcgtac gcggcgcggg cctcggcgaa gccgaaggtg cgatcgatga ccggctcgat
     5701 ctggcgggct tcgagcgcgc ggaccaggcc ctcgaacatc gcgaccgagc cgacgtagat
     5761 cccggcgacg cgaagcccct tgtggaagat gccgtaggtc tggatctcgc ccttggtgcc
     5821 ggtcagcacg ccgagcaggc tcatcgtgcc gccgtagcgc agggccgcga ccgattgatc
     5881 gaacgtgccg gggccgccga cctcgatgac gacgtcggcg ccgcggccgc cggtccacgc
     5941 ccgcgccgcg tccccccact tgggctccgc cttgtagtcg atgacgtggt cggcgcccat
     6001 cgccagggcg cgctcgcgct tggcggcgct cgacgaggtg acgatcacgc gggcgcccgc
     6061 ggccctggcg agctggaggc cgaagatcga gacaccgccg gtaccctgga ccagcaccgt
     6121 gtcgccgggc cgcagctggg tggcctcgaa cagcgcgtgg tacgcggtca cgccggcgca
     6181 cggcagggtc gcggcggcct cgaacgacag ccgggccggc agccggaccc acgcggtctc
     6241 gggcagcacg acctcctcgg cgagcatgcc gtcgacgctg ccaccgagcg ccgcggcgtg
     6301 ggcctgctcg gacatcgcgc cgtcgatcca cctggggaag aacgacgcgg cgacgcggtc
     6361 gccgaccttg tgacgagtga ccgccgcacc aacctcgacg acctcaccgg cgccgtccga
     6421 cgccgggatc accggcgcct tgcgcttcgc ggcaccgcgc gcgatggtga gatcgcggta
     6481 gttgagggag accgcgcgca cgcggacccg cacgtcggtc ggtccgagcg gcggcggcgc
     6541 acgatcgacc agggtcagcg catcgaagcc ctcgcggggc tgaacctcgt aggcccgcat
     6601 cctcatccct cctcggtccg ccgggcgaac ctgcacgaat gggtggtctg catcatcagc
     6661 atggcgccgg atctaccacg tccgatcccg tgagacgcgc gtttcatccg accggccggg
     6721 tggcggccgg accaatgcgc ggcggcacgg cccatgagcc agcgctcact gcccgatgcc
     6781 gcgcttcgcg cggacgcttg ccagatcgcc actgatcgtg agctcgcaca cgggacatcg
     6841 ccagggagcc gatgacatcg gttgtcgatc agctcaccgg ccgtgtgcac gctgcgaaca
     6901 gccggcagca ctgcacccgg cggcgcagag cgtgcttccc aaacggtttt tcgattgcct
     6961 atgctgcgcg ccaacacgca tcggcatcct gcagtgtttt caaggaggga acgatgagac
     7021 gagccctgtt tggcctggtg gcagtcctgg cgattgccgc tttcgtacga cttgcgccgg
     7081 ccgacgatcg cgacgacggc gacgatcacg gcaacgacca gctccagctc gtcgaggcga
     7141 cgatccccca gctcgatcag gcgctgcgct cgcacctgat cacgtccagg cagctggtcg
     7201 agatgtacca ggcgcggatc gcggcgtacg acaaggtcgg ccccacgttc aacgcgttca
     7261 tccacctcaa ccccaacgcc gccgccgagg gcgccgcgcg cgatcgcgag cacggccacg
     7321 agggtgaccg cagcccgatg ttcgggatcc cgatcctgct caaggacaac gtcgacacca
     7381 gcgaccagcc gaccacggcg ggctcgatcg cgctcaccgg ctcggtcccg ccccaggacg
     7441 cgttcatcac caagcggctg cgcgacgccg gcgcggtcat cctgggcaag gccacgctga
     7501 ccgagttcgc gaacttcatc gcgatcggca tgccatcggg ctacagctcg ctcggcggct
     7561 acggcctcaa cccgttcgac ccgcggcccc tgcccggcgg cgacgggcgc ccggtgctga
     7621 cgcccggcgg ctcgagctcg ggccccggga tcgcggtgtc ggccaacctg gtcacgatcg
     7681 cgatcggcac cgagacctcg ggctcgatcc tcagcccgag cacggccaac ggcaacgccg
     7741 ggatcaagcc gacgctcggc ctggtcagcc ggaccggcat cctgccgatc accgccaacc
     7801 aggacacggc cgggccgatc gcgcgcaccg tcactgacgc ggcgatcctg ctcggcgtga
     7861 tcgccggctt cgatcgcgac gatccggcga ccctgccgtg cctcgtgccg ggcaactgct
     7921 tccgcgacta cacgcggttc ctcgacaagc acgcgctcca gggcgcgcgg atcgccgtgc
     7981 cccacgtccc gtactggaac gggttcaacg ccgaccagac cgcgatcatg aacgaggtga
     8041 tcggcgagct gcgcgcggcg ggcgcgttcg tcgatgatcc gcacgagctc cccaaccagg
     8101 ccgacatctc ggcgttcggc atctgcacct cggcgccggc gccgctgacc tgctcgaccg
     8161 tgctgctgta cggccagaag cacgatctca acgcctacct gacccgccgg cccaacgcgc
     8221 cggtccacac gatcgacgac atcatcgcgt tcaacaacgc caacccgatc gccgggctca
     8281 agtacggcca ggcgctgttc ctcgccgccg ccacgctcga caccagcccc ggcagcgccg
     8341 acaccctgcg ggtcaacgcc gaccgcgcca aggacctcca gctgacccag ggcgggatcg
     8401 acgccgtgct caacggcccc gacggcatcg ccggcacggc cgacgacttc gacgcggtcc
     8461 tgttcccgca gaaccgcggc gccgccgccc ccgccaaggc cggctacccc agcgtcgcgg
     8521 tcccgacccg gttccaggtg cccgccggcc cgatcgtcaa ctcgttcccg ttcggcgtcg
     8581 cgttcaccgg ccgggcgttc agcgagcccc ggctgatcgc gctcgcgttc gcctacgagc
     8641 aggcgaccca ccaccgggtg gcgccggcga gcgcgccgcc gctgccgagc gacaccgtca
     8701 cccggcacca cggcgacgac tgaaacactc gacgcggcgt ccgttatccg ttataacgga
     8761 tagcggacat ggccgccacc cagccgcgac gaagctccgc cccgctcccc cagctgtccg
     8821 ggacgacctt cgtctgcgac ccgccccagc ggctcgagct cgagcacggc ttcgagttcc
     8881 agcacggcca gcggatcgcg ccgttcacca tcgtctacga gacctacgg
//
LOCUS       DASVOR010001276         1941 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_480762,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001276 DASVOR010000000
VERSION     DASVOR010001276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480762"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1008)
                     /locus_tag="VF516_24420"
     CDS             complement(<1..1008)
                     /locus_tag="VF516_24420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX8110909.1"
                     /translation="MTSLDDGIELARLVPTGELDELVMSITRALGGELAIFDSRGVRL
                     AGAATAGSPGARRRVEYHGALLVELAASGPAAETSVALCAETLDLLVHHAHARELAAA
                     THEEAMRLTFAELTEHNARLSRAVARLEELDRLKSNFLATMSHELRTPLTSVIGYAEM
                     MAEGLAGPITHDQREYLTTILGKADQLLSLITAVLDVSSLESGQLALERATLSLADLV
                     ASELATFAPQAGRRGIAIQLDACPDAVVVGDRRKIRQVVSSLVSNAVKFTPDRGKVGV
                     ALRRGPLAAHEPAGCGDDAPAIQLVVSDSGIGISRDQVAKIFEPFFQVDSSSTRAFGG
                     TG"
     gene            complement(1005..1673)
                     /locus_tag="VF516_24425"
     CDS             complement(1005..1673)
                     /locus_tag="VF516_24425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEX8110910.1"
                     /translation="MAQVDFAERQVALKLVYYGPPHSGKTSNLRALYDAVDKLNRGRL
                     MTLDTRDDRTLFFDLLPVFFRASGFSFQIKVYTVPGQPVHEATRKVVLAHADAVAFVA
                     DSRPDQREPNRRSWDNLTVNLKALNLEHVPVVVQYNKRDLPEAMALADVDRFGDPARP
                     IHEACAKRGTGVVETFFELVGRAWDALDADRRLSERFGIDTATFRRALADHVGVAEPG
                     KLIE"
     gene            complement(1687..>1941)
                     /locus_tag="VF516_24430"
     CDS             complement(1687..>1941)
                     /locus_tag="VF516_24430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110911.1"
                     /translation="IAERQLDRLRRAAEATLLAGATVRRDAAATPYRATVGVLALLIA
                     TVVIGLVYATLIRGPSDATIAPAGPGPPAPIRSTLQAPAR"
BASE COUNT          281 a          680 c          707 g          273 t
ORIGIN      
        1 gccggtgccg ccgaacgccc gcgtcgacga ggaatcgacc tggaagaacg gctcgaagat
       61 cttggcgacc tgatcgcgcg agatcccgat cccggagtcg gacaccacga gctggatcgc
      121 cggggcgtcg tcgccgcacc cggccggctc gtgcgcggcg agcgggccgc ggcgcagcgc
      181 gacgccgacc ttgccgcgat ccggggtgaa cttgaccgcg ttcgacacca ggctcgacac
      241 gacctggcgg atcttgcggc gatcgccgac cacgaccgcg tcggggcagg cgtcgagctg
      301 gatcgcgatg ccgcgccggc cggcctgggg cgcgaacgtg gcgagctcgc tggcgaccag
      361 gtctgccagc gacagcgtcg cgcgctccag cgcgagctgg ccggactcca gcgacgacac
      421 atcgagcacc gcggtgatca ggctgagcag ctgatcggcc ttgccgagga tcgtggtcag
      481 gtactcgcgc tggtcgtgcg tgatcggccc ggccaggccc tcggccatca tctcggcgta
      541 gccgatcacg ctggtcagcg gggtgcgcag ctcgtgcgac atcgtcgcca ggaagttcga
      601 cttcaggcga tcgagctcct ccagccgggc caccgcgcgc gacagccgcg cgttgtgctc
      661 ggtgagctcg gcgaacgtga gccgcatcgc ctcctcgtgg gtcgccgcgg cgagctcgcg
      721 ggcgtgggcg tggtggacca ggagatcgag ggtctcggcg cacagcgcga cgctggtttc
      781 ggccgccggc ccgctcgcgg cgagctcgac cagcagcgcg ccgtgatact cgaccctgcg
      841 gcgcgccccc gggctccctg cggtcgccgc gccggcgagc cggaccccgc gggagtcgaa
      901 gatcgccagc tctcccccga gcgcgcgggt gatcgacatc accagctcgt cgagctcgcc
      961 ggtcgggacc aggcgagcca gctcgatccc gtcgtcgagc gaggtcactc gatgagctta
     1021 ccaggttctg ctacacctac atggtcggcc agcgcgcgcc ggaacgtcgc cgtgtcgatc
     1081 ccgaaccgct cggacagccg ccgatcggcg tccagcgcat cccacgcccg gccgacgagc
     1141 tcgaagaatg tctcgaccac gccggtgccg cgcttggcgc acgcctcgtg gatcgggcgc
     1201 gcgggatcgc cgaaccggtc gacgtcggcg agcgccatgg cttcgggcag gtcgcgcttg
     1261 ttgtactgca ccaccaccgg cacgtgctcg aggttcagcg ccttgaggtt gaccgtgagg
     1321 ttgtcccacg accggcggtt gggctcgcgc tgatcgggcc gggagtcggc gacgaacgcc
     1381 accgcgtcgg cgtgcgcgag cacgaccttg cgggtcgcct cgtgcaccgg ctgcccgggc
     1441 acggtgtaga ccttgatctg gaacgagaac cccgaggcac ggaagaacac cggcaacaga
     1501 tcgaagaaca gcgtgcgatc gtcgcgcgta tcgagcgtca tcagccgccc gcggttgagc
     1561 ttgtcgaccg cgtcgtacag cgcgcgcagg ttgctggtct tgcccgagtg tggcggcccg
     1621 taatacacga gcttgagcgc gacctgccgc tctgcgaagt cgacttgcgc cacggcgaag
     1681 tctacctcac ctggcgggtg cctgaagggt cgaccgtatc ggggcgggtg gacccggtcc
     1741 ggccggagcg atcgtcgcgt ctgacggacc gcggatcagc gtcgcgtaga ccagcccgat
     1801 gacgacggtg gcgatcagca gcgcgagcac gccgaccgtg gcccggtacg gggtggccgc
     1861 ggcgtcgcgg cggacggtcg ccccggccag cagggtcgcc tcggcggcgc gccgcagccg
     1921 gtcgagctgg cgctcggcga t
//
LOCUS       DASVOR010001277         4162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_101339,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001277 DASVOR010000000
VERSION     DASVOR010001277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101339"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..2280)
                     /locus_tag="VF516_24435"
     CDS             complement(148..2280)
                     /locus_tag="VF516_24435"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEX8110912.1"
                     /translation="MSQAAIGSTADPDPRIIFTQVGETAGIDRTREPASAGPFDGTNT
                     LAYGSWLADLDGDGRLDYYAVNHGQSPHVSGLFLNNGTGGFGPNLFTVGFQSSAANPP
                     NLGNSNEMRFVGDLNGDGLVDFFFLGWSGFGTMCIHQGVAQHADWSGPSFLCYGTADG
                     LAFADVNGDGKLDVLSLDISNFDAYTAYYAQTATYLWRLNNGDPNINHWPTTQNFTAL
                     RITDPNAASAAAPFVDLNNDGIPDKIVGIAQSSSNRGPYGTSTAGQQVFLGQASGGYV
                     LQTATGLESVTRPITRIEDINGDGCLDIGTDLTGYRDNQNWYVQGKAGSTCTVTFTAT
                     PRTALPFYPGFKHYPVDIDNSGLLSQAVIIHVGYGNNDGRPGGVSIYRKLPGGGYTAI
                     PPAQSGININGTYSSEFYADNLSPGDWNDDGKLDLGGTGTPTIPSTDSGFALWTSNLG
                     TTNSWIKVTLPTVTGFFAGAATIEVFDAGFAGDATHLVTPPKLLTTGRAWASQVYHFG
                     IGTRSAVDVRVTFPDGRQVMRTAVAPASRIAVQPMNNAPPTAVATAQPTAVAIGQAVA
                     FDGSGSSDPDGSIAAYRWDFGDGAQATTQAASHAYAAAGGFVATLTVTDDAGSTATSS
                     VTISVADTIAPAVAITGAVFTPTVSDNVGVVKVEWRFDGGLAATATAAPFSYTLNLTP
                     GAGPHTLVARAFDAAGNTTDSAPITIQP"
     gene            2959..3666
                     /locus_tag="VF516_24440"
     CDS             2959..3666
                     /locus_tag="VF516_24440"
                     /inference="COORDINATES: protein motif:HMM:NF042890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6748 domain-containing protein"
                     /protein_id="HEX8110913.1"
                     /translation="MLCALAGCASGVAGDPADDPALEARSTDAYTYFAITADLRKCAS
                     PVCGGWFLSRVNQTTTQCHDGSIAASCYTPVLDWSKSGLTDDQQATMLEACSQGALSS
                     SVYAVVRGQFARRNTTTPRRDLGRFVISEAWLAEGDAMSGGAFVKLRDNGLRCFVAPC
                     PSITELTLNMATTTNIAGLDWTPSAMTDDQIAECNGDLFMPDGLLVVGNRYTMTENGT
                     TADGRTVTVAYQRLLPQ"
     gene            complement(3680..3844)
                     /locus_tag="VF516_24445"
     CDS             complement(3680..3844)
                     /locus_tag="VF516_24445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110914.1"
                     /translation="MNRQPRDHGADRGEPAPPAGAEASLHPRFRPALRGHIARRHLRP
                     AADPPPPLPV"
     gene            complement(3841..>4162)
                     /locus_tag="VF516_24450"
     CDS             complement(3841..>4162)
                     /locus_tag="VF516_24450"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEX8110915.1"
                     /translation="PVKPLAEPVAAAEVTRMPLPLRECPAKYTEEARGASVEGVVVLA
                     AIIDEHGRARDITVVQGLPHGLTQSAIAELTRCPFSPGERNGQPVPVRLREIKFRFVL
                     AEGP"
BASE COUNT          609 a         1401 c         1484 g          668 t
ORIGIN      
        1 tcgcgtacct gtcggcgctg atggcgcgca acgacggcaa catcacgcgc tcggcccagg
       61 aggccggctt gacccgctac cacctgcgcg agctgctcaa gcgccacggg ctcaagggcg
      121 gcgacgccga gtagcctggg gtctaggcta gggctggatc gtgatcggcg cggagtcggt
      181 ggtgttgccg gccgcgtcga acgcgcgcgc caccagcgtg tgcggacccg cccccggcgt
      241 caggttgagc gtgtaggaga acggggccgc cgtcgcggtc gcggccagcc cgccgtcgaa
      301 ccgccactcg accttgacca cgccgacgtt gtcggacacg gtcggcgtga acacggcgcc
      361 ggtgatcgcg accgccggcg cgatggtgtc ggcgaccgag atcgtcaccg agctggtggc
      421 ggtgctgccg gcgtcgtcgg tgacggtcag cgtcgcgacg aacccgccgg ccgcggcgta
      481 ggcgtggctc gccgcctggg tcgtggcctg ggcgccgtcg ccgaagtccc agcggtacgc
      541 ggcgatcgag ccgtcggggt cactcgagcc cgagccgtcg aacgccaccg cctggccgat
      601 cgcgaccgcg gtcggctggg cggtggcgac cgcggtcggc ggagcgttgt tcatgggctg
      661 gaccgcgatc cggctcgccg gtgcgacggc cgtgcgcatg acctgccggc cgtcggggaa
      721 cgtcacccgg acgtcgaccg cgctgcgcgt gccgatcccg aagtggtaga cctggctcgc
      781 ccacgcccgg ccggtggtca gcagcttcgg cggcgtgacg aggtgggtcg cgtcgcccgc
      841 gaacccggcg tcgaacacct cgatcgtcgc ggcgccggcg aagaacccgg tgacggtcgg
      901 cagcgtgacc ttgatccagc tgttggtcgt gccgaggttc gaggtccaca gcgcaaaccc
      961 gctgtcggta ctggggatcg tcggcgtgcc ggtgccgccg aggtccagct tgccgtcgtc
     1021 gttccagtcg ccgggcgaca ggttatcggc gtagaactcg ctgctgtagg tgccgttgat
     1081 gttgatgccg ctctgggccg gtgggatcgc ggtgtagccg ccaccgggga gcttgcggta
     1141 gatgctcacg ccgcccggcc ggccgtcgtt gttgccgtag ccgacgtgga tgatgaccgc
     1201 ctgcgacagc agaccgctgt tgtcgatgtc caccggatag tgcttgaagc cggggtagaa
     1261 cggcagcgcg gtccgcggcg tggccgtgaa cgtgacggtg caggtcgagc cggccttgcc
     1321 ctggacgtac cagttctggt tgtcgcggta gccggtcagg tcggtgccga tgtcgaggca
     1381 gccatcaccg ttgatgtcct cgatccgcgt gatcggccgg gtgacggact ccaggccggt
     1441 ggcggtctgc agcacgtagc cgccgctggc ctggccgagg aacacctgct gtccggccgt
     1501 cgacgttcca tacggtccgc ggttgctcga cgactgggcg atcccgacga tcttgtccgg
     1561 gatgccgtcg ttgttgagat cgacgaacgg cgccgccgcg gaggcggcgt tcgggtcggt
     1621 gatccgcagc gcggtgaagt tctgggtggt cggccagtga ttgatgttgg gatcgccgtt
     1681 gttgagtcgc cacagatacg tcgcggtctg ggcatagtac gcggtgtacg cgtcgaaatt
     1741 ggaaatatcc aggctgagca catcgagctt gccgtcgccg ttgacgtcgg cgaacgccag
     1801 gccgtcggcg gtgccgtagc acagaaagct cgggccgctc cagtcggcgt gctgggcgac
     1861 gccctgatgg atgcacatcg tcccgaaacc gctccagccc aggaagaaga agtcgaccag
     1921 tccgtccccg ttgagatcgc cgacaaaccg catctcgttg gagttaccga ggttcggcgg
     1981 attggcggcc gacgactgga agcccacggt gaacaggttc gggccgaacc cgccggtgcc
     2041 gttgttgagg aacagccccg acacgtgcgg agactgaccg tggttcaccg cgtagtagtc
     2101 gagccggccg tcgccgtcga gatcggccag ccacgagccg taggcgagcg tgttcgtccc
     2161 gtcgaacgga ccggcggagg ccggctccct ggttcggtcg attcccgccg tctctccgac
     2221 ctgcgtgaag atgatgcgcg gatccggatc cgccgtactt ccgatcgcag cctgcgacac
     2281 cgcaccgacg tcttcccctg gatcgcaggc gcatggtagt gcgagcccga gcgcaaggac
     2341 tgtctttgct ctcatcattc cccttccttt ccatccggag tcgctccgga ctgaccccgc
     2401 ctcataccct cccgacacaa cagacctgcc cgctggtcag gctcgatgca tgagcagggt
     2461 gctcattgcg ttgctgactc cccggggcaa caacgaacat attcattcga cgtaaacgag
     2521 cgcccaatag cagatctccc gcagccgacc tatcgcaatg cgtatgccag tgccggctgc
     2581 gagcgcagtg ctcccgtctt cattacgtat ccggacccgt gctgaaggcg cggcgcacgg
     2641 ctcgatccgg acatcgcgat tgcgttccgg tgaggagtgt gcagcgccag gtaaggcaga
     2701 acacgggcgc cgcgcgtcca gatcggggcg tgccgtgcgc tcggcgcccg acctcgtcag
     2761 gccggacggc cggcggcgga atgcgttcgc gcacccggtg cagccggcgg acgccgcggc
     2821 gaaaaccgtt cgtaatttcg agccgatctg cgcgcggcag cacggcccgc tgtttgctct
     2881 gtccgtcggc agggcatttc aagtcagggg gcaatcgcca tgcgttcact actcgggttc
     2941 acgacactgc catccgcctt gctatgtgcg ctcgcggggt gcgcatcggg ggtcgcgggc
     3001 gatcccgcgg acgatcccgc gctcgaggcc aggtcgaccg atgcgtacac ctacttcgcg
     3061 atcaccgcgg atctccgcaa gtgcgcgtcg ccggtgtgcg gcggctggtt cctcagccgg
     3121 gtgaaccaga cgacgacgca gtgccacgac ggcagcatcg cggcgtcgtg ctacacgccg
     3181 gtcctcgact ggtcgaagtc cggcctgacg gacgaccagc aggccaccat gctcgaggcg
     3241 tgcagccagg gcgcgctgtc gtcgagcgtc tacgcggtgg tgcgcggcca gttcgcgcgc
     3301 cgcaatacca cgacgccgcg tcgagatctc ggccggttcg tgatcagcga ggcctggctc
     3361 gccgagggcg acgcgatgtc cggcggtgcg ttcgtcaagc tccgcgacaa cggcctgcgc
     3421 tgcttcgtgg cgccgtgccc gagcatcacc gagctgacgc tcaacatggc caccacgacc
     3481 aacatcgccg ggctcgactg gacgccgtcc gcgatgaccg acgatcagat cgcggagtgc
     3541 aacggcgatc tgttcatgcc cgacgggctg ctggtcgtgg gcaaccgcta cacgatgacc
     3601 gagaacggca ccaccgcgga cgggcgcacc gtgaccgtgg cgtaccagcg gctgctcccg
     3661 cagtgacggt gcatgccggt cacaccggca gcggcggtgg cgggtcggcc gcgggacgca
     3721 ggtggcgccg ggcgatgtga ccgcgcagcg ccgggcgaaa ccgcggatgc aggctcgcct
     3781 ccgcgccggc cggtggcgcc ggctcgccgc gatcggcgcc gtgatcgcgc ggctggcggt
     3841 tcatggaccc tccgccagga cgaagcggaa cttgatctct cgcagacgga ccgggaccgg
     3901 ctggccgttg cgctcgccgg gcgagaacgg gcaccgggtc agctccgcga tcgccgactg
     3961 ggtcagcccg tgcggcaggc cctggaccac ggtgatgtcg cgggcgcggc cgtgctcgtc
     4021 gatgatcgcc gccagcacga cgacgccctc gaccgaggcg ccgcgcgcct cctcggtgta
     4081 cttggccgga cactcgcgca gcggcagcgg catcctggtg acctcggccg cggcgaccgg
     4141 ctcggccagc ggcttgaccg gc
//
LOCUS       DASVOR010001278         5413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421513,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001278 DASVOR010000000
VERSION     DASVOR010001278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421513"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VF516_24455"
     CDS             <1..544
                     /locus_tag="VF516_24455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110916.1"
                     /translation="SSGASGGGHDGSGPRRVYVAADPVQLEVYRTQYPDVARQPDETP
                     VFRYAETTAVPDNGVSYWQTNAPHTDCTSHVRIETTMGELRRSGEVRIDPLAPEATGE
                     SLVVLHTPGTTTVPGTTRRVLGRAGAQGTGAAVLEGEVPSGLWRDWDGNIHDGPDLFR
                     AAPSRGSGSPPRDSDPGGTR"
     gene            complement(638..2650)
                     /locus_tag="VF516_24460"
     CDS             complement(638..2650)
                     /locus_tag="VF516_24460"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8110917.1"
                     /translation="MSRSLRCVALGLVPLLGTCCPTLWVQVEKDSPSPWPSVPLPDMD
                     QTPYTMPSTAPNRFDVLESGRPTVAAPGYTDTFDPQNSFNNALARSIADLNWIQVQYG
                     HPVTRLCEPDHLFRGKLSCARPELTGDAAGCHGVAFSQPVPKAVTVVLGGRRFAVVGS
                     DTKAIDLSESVRRAPGHDDVEVCFESTLELSHSLLSFIHLSDIQLRDPSVTLTDRRLS
                     HALDWFDALSSFEYDEDLAFYNQYVVEALFATINTIVEQTLDAERPKFVIHSGDSIDA
                     GAIGELDRFHSLIDRLRIPFYQLLGNHDVLVFGNLTPTSKYDTDSACTPIEALIGSKT
                     WVAPNKLCVDQRVLCPTCVGDERQLVALDTQEQTRQHFIGRLRHVRADRLAEPARAEA
                     GWYCDKSSPKVRNDPYTRLHGFDLGTVDNQLDLGMVKDAPDSRPLGYYAFAQRLEADR
                     VDCQGCVKFGRHVLFIALNTEDLEDHVGGIGGRVKHDQLQWLKDVLSCVQDVPAHRRD
                     LVLVFGHQPLSLIEVEPRDVDPKQPEHTVARILEEASPNVVGYLYGHHHEHSICGDVA
                     RKDVCSKFWEVETASLVEFPQEGRLVRIKEISGDLGFLELTALRERLASPDSPLARYV
                     ALARRGAERDFCFTQREKPRGRCSPDQRPYRTDGRNANARLFFRMP"
     gene            complement(2647..3477)
                     /locus_tag="VF516_24465"
     CDS             complement(2647..3477)
                     /locus_tag="VF516_24465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110918.1"
                     /translation="MLAVDDTRSHGPSARNWFDAAVDAQVIAGAHDDTVLGLVEQLSI
                     GAPTSSDRDLHDILERFQPYQLRLRSTTFRGPAGWDQGPLTLGFQRYFPIDHVSFLPL
                     AYAHFGVEAVVSTPWLSGRHVVPPTAVRIADAVETELAQNGWSLRPLSGYVRADFLAC
                     RSFFGEAGAAPELFVPSGGSTEYDARFHVALGWSFGCEHRLVDHRPKFSVEYRVRLRL
                     YQGDAGVSYSDSLGLGLQFDCGALVMQVLASTNLSEQPLSYGILGLRVQLGPRKSSPP
                     "
     gene            complement(4536..>5413)
                     /locus_tag="VF516_24470"
     CDS             complement(4536..>5413)
                     /locus_tag="VF516_24470"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8110919.1"
                     /translation="GSLNNDRAIDPSIQERIQAGQEVRTFRISLSIMGDGFVEAIDSN
                     TLVAIAAAQPSGQRGTVIQVPVTEAPGQVRVGRFGWKNQNSSLLSFAADAYVTEMGIT
                     SPLQPTENTSNGRSVAAFDTVTDPEDTGADIASFTLFMRSLKAPSVDAAQAATASAQR
                     GSQLFDALGCSVCHVRSINTVTAGTVINGGQFPVPAALGDKTIHPFGDFLLHDVGTGD
                     GIVQNGGQGTRNLVRTAPLWGLRARDRLMHDGATVSRTDAILRHGGQANGSATSFAFL
                     STSSQNDVINFLNSL"
BASE COUNT          868 a         1834 c         1863 g          848 t
ORIGIN      
        1 gagcagcggt gcctccggcg gcggccacga cggctccggc ccgcgccggg tctacgtcgc
       61 cgccgacccg gtgcagctcg aggtctaccg cacccagtat cccgacgtcg cgcggcagcc
      121 cgacgaaacc ccggtcttcc gctacgccga gaccaccgcc gtcccggaca acggcgtctc
      181 gtactggcag accaatgcgc cgcacaccga ttgcaccagc cacgtgcgca tcgagaccac
      241 gatgggcgag ctgcggcgct ccggcgaggt ccgcatcgac ccgctcgcgc ccgaggccac
      301 cggcgagtcg ctcgtcgtgc tccacacccc cggcaccacg accgtgcccg gcaccacgcg
      361 ccgcgtcctc ggccgcgccg gcgcgcaggg caccggcgcc gcggtgctcg agggcgaggt
      421 cccgtcgggg ctgtggcgcg actgggacgg caacatccat gacggcccgg atctgttccg
      481 cgccgcgccc agccggggca gcggctcccc gccgcgcgac agcgaccctg gaggcacgcg
      541 atgagcgccc ccgccgtact cgtgctgtcc gagccgggcg gcagccccgc cgccgacgtc
      601 gccctcgatc gcttcaccga cgagccgctc gggcgcgcta tggcatccgg aagaacagcc
      661 gggcattcgc gttgcggcca tcggtacggt acggcctctg gtccggactg cagcgccccc
      721 gcggcttctc gcgctgggtg aaacagaagt cgcgctcggc gccgcgtcgc gcgagggcca
      781 catagcgcgc cagcgggcta tccggactgg cgaggcgctc acgtagcgcg gtcagctcca
      841 ggaacccgag gtcgccgctg atttccttga tgcgtaccag gcggccctcc tgcgggaact
      901 cgaccaggct cgcggtctcg acctcccaga acttcgagca cacatccttg cgagcgacgt
      961 cgccgcagat cgaatgctcg tggtggtggc catacaggta gccgaccacg ttcgggctcg
     1021 cctcctccag gatcctggcg accgtgtgct ccggctgttt cggatcgacg tcccgcggct
     1081 cgacctcgat caggctcagc ggctgatgcc cgaacacgag cacgaggtcg cgccggtgcg
     1141 cgggtacgtc ctggacgcag gacagcacat ccttcagcca ttgcagctgg tcatgcttca
     1201 cccgtccgcc gatcccaccg acgtgatcct cgaggtcctc ggtgttcagc gcgatgaaca
     1261 gcacgtggcg accgaacttg acgcatccct ggcagtctac acgatcggcc tcgagccgct
     1321 gcgcgaacgc atagtagccg agtggccggc tatcgggggc gtccttgacc atccccagat
     1381 cgagctggtt atcgaccgtc cccagatcga agccatgcag gcgcgtatag gggtcgttgc
     1441 ggaccttcgg actcgacttg tcgcagtacc agccggcctc ggcgcgggcg ggttccgcca
     1501 gccggtctgc tcgaacatgc cgcaggcgac cgatgaagtg ctgccgcgtc tgttcctgag
     1561 tatcgagcgc gaccagctgc cgttcgtctc cgacgcaggt cggacacaag acacgttgat
     1621 cgacacaaag cttgttcggc gccacccagg tcttgctgcc aatcagggcc tcgatcggcg
     1681 tgcaagcact gtcggtgtca tatttgctcg tcggcgtgag gttgccgaac accagcacgt
     1741 cgtggttgcc cagcagctgg tagaacggga tccggagccg gtcgatcaac gagtggaagc
     1801 ggtcgagctc accgatcgcc ccggcgtcga tggaatcacc gctgtggatg acgaacttcg
     1861 gcctctccgc gtccagggtt tgctccacga tggtgttgat cgtcgcaaac aacgcctcga
     1921 caacgtactg attgtagaat gccagatcct cgtcgtactc gaacgacgac agcgcgtcga
     1981 accagtcgag cgcgtggctg agccggcggt cggtcagcgt gaccgatgga tcgcgcagct
     2041 ggatgtccga caggtgaatg aacgatagca gtgagtgcga gagctccagc gtcgattcga
     2101 agcagacctc gacgtcatcg tgtccaggcg cccgtcgcac ggactcggac aggtcgatcg
     2161 ccttcgtgtc cgagcccacg accgcgaagc gccgtccacc gagcacgacg gtcacggcct
     2221 tgggcacggg ctgcgagaac gctaccccgt ggcagccggc ggcgtcgccg gtgagctccg
     2281 gacgcgcgca gctgagcttg ccacgaaaca gatggtcggg ctcgcagaga cgcgtcacag
     2341 ggtgaccgta ctgcacctgg atccagttga gatcggcaat ggaacgggca agagcgttgt
     2401 tgaaactatt ctgaggatcg aacgtgtcgg tgtagccggg cgcggcaacc gtcgggcggc
     2461 cgctctcgag cacgtcgaac cggttcggtg ccgtggacgg catcgtgtac ggcgtctggt
     2521 ccatgtccgg gagcggcacg gacggccatg gcgacgggct gtccttctcg acctggaccc
     2581 acagcgtggg acagcacgtc ccgagcagcg ggaccaggcc gagcgccaca cagcgcaagc
     2641 tccggctcat ggcggtgagc tcttcctggg gcccagctgc acccgcagtc cgaggatccc
     2701 gtaggacagc ggctgctcgc tgaggttggt cgatgccagc acctgcatca ccagcgcacc
     2761 gcaatcgaac tgcaagccga gtcccagcga gtcgctgtac gagacccctg cgtccccctg
     2821 gtagagccgc aggcggaccc ggtactcgac ggagaacttc gggcgatggt ccacgaggcg
     2881 gtgctcacag ccgaacgacc atccgagcgc cacgtggaac cgggcgtcgt actcggtgct
     2941 cccgccgctt ggcacgaaca gctccggcgc cgcgccggcc tcgccgaaga acgaacggca
     3001 agcgaggaag tcggcgcgga catagcccga cagcggtcgc aggctccagc cgttctgcgc
     3061 cagctcggtc tcgacggcgt ctgcgatgcg cacggcggtc ggtggaacca cgtgccggcc
     3121 cgagagccat ggcgtcgaga ccaccgcctc gacgccgaag tgcgcatagg ccagcggcaa
     3181 gaacgagaca tggtcgatcg ggaagtaccg ctggaacccg agcgtcagcg gcccctgatc
     3241 ccagccagcc ggaccgcgaa acgtggtcga gcgcagccgg agctggtagg gctgaaaccg
     3301 ctcgaggatg tcgtgcagat cgcggtcgct cgacgtcggt gctccgatgc tcagctgctc
     3361 gaccaggccc aggaccgtgt cgtcgtgcgc gccggcgatc acctgcgcgt caaccgcggc
     3421 gtcgaaccag tttcgcgcgc tcggtccgtg gcttcgcgtg tcgtccaccg cgagcaccgg
     3481 cgtcgcctgg gggagcggat cgggctggtc cgctgtcccg gcccaggcgc tcctcgagca
     3541 tagcgcgctc gcgacgagca gcgcgctcgc gaaaccttgc ctcgacggcc tcgaatgacg
     3601 tcccctgcgg gaagcaatca cagtcatgtc ccgtgctttt cctgtgcgag tctcgcagat
     3661 cccagcatgc agagagctcc agcccgccct cgagccgacc ctggttggca cagatgaggt
     3721 cgtagggcgt ctcgctgctg gcgagccgtc ccggtggacc gatcgcagcc acccgtgaca
     3781 gggcgctctc gggtttggct ttgaccttgg cgcgagcgcc tgtcggcttc ttgaccgcag
     3841 cgcgcttggc cttccgcccg cgttggggcc tggcattctt gctgcgacgc ttgtgtggct
     3901 ggcttctggg catgatgttt cgcctggcga gctgtagcgg ggctcggcat cggcgatgac
     3961 agcccgatag ccaaagcgac gaggatatgc aacgaacgac atcaccgcgc gtgctgtcca
     4021 gcagcgcccc acctggtcat ctggccaccc tagcaggcat cgtcgccggc tatccagatc
     4081 gggagccgca ttttgcaatc atcgcttctg cctgcagaca acccactcag caggcggcga
     4141 tggtccgcgc actgaccagg atggcgccga gtcctacctt gtcatacatc atcggcgccg
     4201 gaccgtcccg gccgggggtg gatggcgagg cacgatgaga tcgggagcgc tcgacttcaa
     4261 tccagatctg gaaacctcgc cgagatgacg ccgccgcggc gcatcgtgca gaccttcgag
     4321 tgggacggta tgcccggcca cgtcgtgatc aacgagacct ggggcggtga ccgtcgcgtg
     4381 gcggatcgag aagcacggcc tggagccatg cgggcagcgc atgtacccgt cgagcatccg
     4441 tcgcgtccgc ggtgtccagc gcgggcgcgg cggcaggccg tgcttctcca tccgccgggg
     4501 aagccccggc gccgtcgagc gagcgaggag cgcggctaca gcgagttcag gaagttgatc
     4561 acgtcgttct gcgaggaggt cgacaggaac gcgaaggagg tggcggagcc gttggcctgg
     4621 cccccgtggc ggaggatcgc gtcggtgcgc gacaccgtgg cgccgtcgtg catcagccgg
     4681 tcgcgcgcac gcaggcccca cagcggcgcg gtgcgcacca ggttgcgggt gccctggccg
     4741 ccgttctgga cgatgccgtc gccggtgccg acgtcgtgga gcaggaagtc gccgaacggg
     4801 tggatggtct tgtcgccgag cgcggccggc acggggaact ggccgccgtt gatcacggtg
     4861 cccgcggtga ccgtgttgat gctgcggacg tggcagaccg agcagccgag cgcgtcgaac
     4921 agctgcgagc cgcgctgggc cgaggcggtc gcggcctggg cggcgtcgac cgacggggcc
     4981 ttgagcgagc gcatgaacag cgtgaagctg gcgatgtcgg cgccggtgtc ctcgggatcg
     5041 gtgacggtgt cgaacgcggc caccgagcgg ccgttggagg tgttctcggt cggctgcagc
     5101 gggctggtga tgcccatctc ggtgacgtag gcatcggctg caaacgacag caggctggag
     5161 ttctggttct tccagccgaa tcgcccgacg cggacctggc cgggggcttc ggtcaccggg
     5221 acctggatca cggtgccgcg ctggccggag ggctgggcgg cggcgatcgc gacgagcgtg
     5281 ttgctgtcga tcgcctcgac gaagccgtcg cccatgatgc tcaggctgat ccggaacgtg
     5341 cggacctcct ggccggcctg gatccgctcc tggatcgacg gatcgatcgc gcggtcgttg
     5401 ttgagcgagc cgc
//
LOCUS       DASVOR010001279         2383 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_334367,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001279 DASVOR010000000
VERSION     DASVOR010001279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2383
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334367"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="VF516_24475"
     CDS             complement(<1..488)
                     /locus_tag="VF516_24475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110920.1"
                     /translation="MARDVTHRYVDPLAQVWLAAARRIGLRVVRTPDAYAATDGHGTL
                     AVGDDATLDADDSLAQMIFHELCHSLVEGEDAFARPDWGMDNTGPDHDWREHACLRVQ
                     WVLAGRHGLRGLFAPTTDFRAFWDRLAGDVLADRADPSVQAAIAGLGRVALPPWAPAL
                     DEA"
     gene            561..797
                     /locus_tag="VF516_24480"
     CDS             561..797
                     /locus_tag="VF516_24480"
                     /inference="COORDINATES: protein motif:HMM:NF017355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEX8110921.1"
                     /translation="MSKMIQVRNVPDQLHRKLKIRAVEQGVSLSDYILAELRRLAERV
                     SPQELAERARSIVREDFTPSPAELLRAERDQRSS"
     gene            794..1192
                     /locus_tag="VF516_24485"
     CDS             794..1192
                     /locus_tag="VF516_24485"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8110922.1"
                     /translation="MIVIDASALVEIVIGGTPRAARLAARIADPSESLHAPHLIDLEV
                     TLVLRSLVARQQLSSALATRAIGDLLALDITRYPHDVLVPRIWQLRGNLTVYDAAYIA
                     LAESLRAPLVTCDGQLAAAPGNRAQVELFA"
     gene            1481..>2383
                     /locus_tag="VF516_24490"
     CDS             1481..>2383
                     /locus_tag="VF516_24490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110923.1"
                     /translation="MGSLALSATTPAAAGPRVASHDKQRPGDGKRVDRKQACARYVGG
                     EGAVSAWVYADATGALAYKPLDEHGDHIMDFSSAGYGGGGVAIPTIAAARRVKPSGED
                     DTATIQAAIDAVSRLPLRDGFRGAVELAPGTFTLAGKLTITTGGVVLRGSGTGRTGTA
                     ITVVGPERRVLDIRGSGVATAAPGATPLAITDAYVPSGAIRLHVHDAQALSVGDAVLV
                     QRPVTEGWVRFLGMDKLVRDGRPQVWIPAGTLHAWERTVAAIDGDEITLDIPLSDSID
                     QRQVSPPGATVVKASYPGRITHVGL"
BASE COUNT          353 a          801 c          906 g          323 t
ORIGIN      
        1 gcctcgtcga gcgccggggc ccacgggggc agggcgacgc ggccgaggcc ggcgatcgcg
       61 gcctggaccg acggatcggc gcggtcggcg agcacgtcac cggcgagccg atcccagaac
      121 gcgcggaagt cggtggtcgg cgcgaacagg ccgcgcaggc cgtggcggcc ggcgagcacc
      181 cactgcacgc gcaggcaggc atgctcgcgc cagtcgtgat cggggccggt gttgtccatg
      241 ccccagtcgg gccgcgcgaa cgcgtcctcg ccttcgacca gcgagtggca gagctcgtgg
      301 aagatcatct gggcgaggct gtcgtcggcg tcgagcgtcg cgtcgtcgcc gaccgcgagg
      361 gtgccgtggc cgtcggtggc cgcgtaggcg tcgggggtgc gcaccacgcg caagccgatc
      421 cggcgcgcgg cggcgagcca gacctgcgcc agcggatcga cgtagcggtg ggtgacgtcc
      481 cgggccacgc ctgcatctag catggttcca gcgccgggag cgagcttgac atgcgctgca
      541 catgtaaaca tgctgatcac atgtcgaaga tgatccaggt ccggaacgtc ccggatcagc
      601 tccatcgcaa gctcaagatc cgggcggtcg agcagggagt ctcgctgtcc gactacatcc
      661 tggcggagct tcgccggctc gccgaacggg tatcgccgca agagctggcg gagcgagcac
      721 ggtccatcgt gcgtgaagac ttcaccccat cgcctgccga gctgctccgg gccgaacgag
      781 atcagcggtc gtcatgatcg tgatcgacgc gtccgcgctc gtcgagatcg tgatcggtgg
      841 gaccccacgg gcagcccggc tggcagcgcg catcgcggac ccgtcggaat ccctgcatgc
      901 gcctcacctg atcgatctcg aggtgacgtt ggtgctccgc agtctcgtgg cgaggcagca
      961 gctgtcgagc gcactggcga cccgcgcgat cggcgacctc ctggcgctcg acatcacgcg
     1021 gtatccgcac gatgtgctgg tgccacgaat ctggcagctg cgtggaaacc tcacggtgta
     1081 cgatgcggcg tacatcgcgc tcgccgagag cttgcgcgcg ccgctcgtga cgtgcgacgg
     1141 ccagctcgcc gcagcaccgg gcaaccgggc gcaggtcgag ctgttcgcgt gacccgcacg
     1201 gtccgcggaa gcggaccctg tccgctgcag cggaccgtcg cgaacgccgc ggatctgtga
     1261 cggcgccgga aatccgctgg atggcggcga aggcagggat cgcgcgccgg catcgcgctt
     1321 gctcgtggca ggggtgcggg gccatcgatc gcgccgcgaa ccgctatcga aacaaggggt
     1381 atgcacgatg ttcgagaaca cgacgcagag agaggtctcc atcggccgga gcgcggctgc
     1441 caggtggcgg agccgcatcg ccggtctcac gatgctggtc gtgggcagtc tcgcgctgtc
     1501 cgccaccacg cctgccgcgg cgggtccccg ggtcgcatcg catgacaagc agcgtccggg
     1561 cgacggcaag cgggtcgacc gcaagcaggc gtgcgcgcgg tatgtgggcg gcgagggcgc
     1621 ggtcagcgcc tgggtgtacg ccgatgcgac cggcgcgctc gcctacaagc cgctcgacga
     1681 gcacggcgac cacatcatgg acttctcgtc ggcgggatac ggcggtggcg gcgtggcgat
     1741 cccgaccatc gcggccgcgc ggcgggtcaa gccgtcgggc gaggacgaca cggcgacgat
     1801 ccaggcggcg atcgatgcgg tgtcgcggct gccgctgcgc gatggctttc gcggcgcggt
     1861 ggagctggcg ccgggcacgt tcacgctcgc cggcaagttg acgatcacca ccggcggcgt
     1921 cgtgctgcgc ggcagcggca cggggcggac cggcaccgcc atcacggtgg tcggcccgga
     1981 gcggcgcgtg ctggacatcc gtgggagcgg ggtcgcgacg gcggcgcccg gcgccacgcc
     2041 gctggcgatc accgacgcct acgtgccgtc gggcgcgatc cggctccacg tccacgacgc
     2101 gcaggcgctg tcggtcggcg acgcggtgct ggtccagcgc ccggtgaccg aggggtgggt
     2161 gcgcttcctg ggcatggaca agctggtgcg cgacggaagg ccgcaggtct ggatcccggc
     2221 cgggacgctc cacgcgtggg agcgaacggt ggccgcgatc gacggcgacg agatcacgct
     2281 cgacatcccg ctgtcggact cgatcgacca gcgccaggtc agcccgccgg gcgcgaccgt
     2341 cgtgaaggcg agctacccgg ggcggatcac gcacgtgggg ctg
//
LOCUS       DASVOR010001280         2126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_42526,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001280 DASVOR010000000
VERSION     DASVOR010001280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42526"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..196
                     /locus_tag="VF516_24495"
     CDS             <1..196
                     /locus_tag="VF516_24495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110924.1"
                     /translation="EAERAAAERDVSFVKQTGGAEDLAATQAIQRSLASGANEVFTFG
                     RHEGAIAHFHRALDAALAAL"
     gene            400..1149
                     /locus_tag="VF516_24500"
     CDS             400..1149
                     /locus_tag="VF516_24500"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8110925.1"
                     /translation="MYYEIHGDRDGGGTPLVLLHGGGSTIDVTYGRVLPLFARHRTVI
                     AVEEQGHGRTTDRDAPVRFDTSADDVAALLGQLQVEKADIMGFSNGASVAMQVAIRHP
                     ALVRKLVFASSMTKQSGAAPQFWEFIARATFDDMPQPLKDAFLAVNPDPRKLRAMHDK
                     DLERMRHFAEISDDDVRKVNAPTLILLGDRDIPTPEHAVELTHLLPHPHLVVLPGGHG
                     DYLGELIAAKQPSRTPPWTVGLIEDFLDSAG"
     gene            1509..>2126
                     /locus_tag="VF516_24505"
     CDS             1509..>2126
                     /locus_tag="VF516_24505"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110926.1"
                     /translation="MQPGDSEPAAVATSAADASAIGPLDELQRRGRYLLLGRLGSGGM
                     GVVHAAYDPTLDRKVALKLLRPGDGRDDDSVARGRLLREARALARLSHPNVVHVYEVG
                     TLEDKQIYIAMEHIEGQTLRAWQKEARRPFRDVLARYRAAGRGLQAAHEAGLVHRDFK
                     PDNVILGKDGRVVVLDFGLAVAPEVGVPGPEAEAAPAPPVNTTSDS"
BASE COUNT          351 a          712 c          750 g          313 t
ORIGIN      
        1 cgaggccgag cgcgcggccg ccgagcgcga cgtcagcttc gtgaagcaga ccggcggcgc
       61 ggaggatctc gcggccaccc aggcgatcca gcgcagcctc gccagcggcg ccaacgaggt
      121 gttcacgttc gggcgccacg agggggcgat cgcccacttc caccgcgcgc tcgacgccgc
      181 gctggccgcg ctgtgacggc ggcggcatca gctccggtcg cgcgcgccta cgccaatgct
      241 cagaaccatg ggcagggagc tgtgtcttgt tcgtcgagtg tcattgcttg cgtgctcctg
      301 gcgattggct gtcatcctgc tcacgaggag aagtcgacca tgacaacgag cgatgcgggt
      361 gtaacgaaga gtggccatct gccggtacat gggatccgca tgtattacga gatccacggt
      421 gaccgtgacg gtggcggcac tccgctcgtg ctgctgcacg ggggtggctc gacgatcgac
      481 gtgacgtacg ggcgcgtcct gccgctgttc gcgcgacacc gcacggtgat cgccgtcgag
      541 gaacagggcc acgggcggac caccgatcgc gacgcgcccg tgcgcttcga tacgtcggcc
      601 gacgacgttg cggcgctgct cggccagctg caggtcgaga aggccgacat catggggttc
      661 agcaacggcg ccagcgtggc catgcaggtc gcgatccggc atcccgcgct ggtgcgaaag
      721 ctggtgttcg cgtcgtcgat gacgaagcag agcggggcgg ctccgcagtt ctgggagttc
      781 atcgccaggg cgacgttcga cgacatgccg cagccgctca aggacgcgtt tctggcggtc
      841 aacccggacc cgcggaagct tcgcgcgatg cacgacaagg acctcgagcg gatgcggcac
      901 ttcgccgaga tcagcgacga cgacgtgcgc aaggtgaacg ctcccacgct gatcctcctg
      961 ggcgatcgcg acattcccac gcccgagcat gcggtggagc tcacccacct cttgccccat
     1021 ccgcatctcg tcgtcctgcc cggtggacat ggcgactatc tgggggagct gatcgccgcg
     1081 aagcagccgt cgcgcactcc gccatggacc gtcggcttga tcgaggactt cctcgatagc
     1141 gccggatgac gagccgcagc gtcgcggtgg tcagtccggc cgacgcggtc gcacctggga
     1201 catacgcgcg cagccgctca ccccgagcac gatgccaaga aggatgctga gcgcggctcg
     1261 tcgcggcatg accgaagcga ccgttgcatg gaagaaacgc gccaagtcgg tgaaaagtcg
     1321 cgcacctgct gacggagccc gcagcgggcc tgggcggcga gcggcgtcgg atacgaggtc
     1381 atctccaagc agacgaaggt gctcctcact ctggctcatc cgcgttcatg gccattcgca
     1441 aatagtcaat gacaacggct atgatggtcc aggagcctcg cttggacaca ggggagcacg
     1501 acagacccgt gcaaccgggt gacagcgagc ccgctgcggt cgccacgtcg gcggccgatg
     1561 cctccgcgat cggcccgctc gacgagctgc agcgccgcgg ccggtacctc ctcctgggac
     1621 ggctgggcag cggcggcatg ggcgtcgtcc atgccgcgta cgatccgacc ctcgaccgca
     1681 aggtcgcatt gaagctgctc cgtccgggcg acggtcgcga cgacgactcg gtggcgcgcg
     1741 ggcgcctgtt gcgcgaggcc cgggcgctgg cgcgactgtc gcacccgaac gtcgtccacg
     1801 tgtacgaagt cggcacgctc gaggacaagc agatctacat cgcgatggaa cacatcgagg
     1861 ggcagacgct gcgggcgtgg cagaaggagg cgcggcggcc gttccgcgac gtcctggcgc
     1921 gctatcgcgc ggcgggccgc ggtctgcagg cggcgcacga ggccggcctg gtccatcgcg
     1981 acttcaagcc cgacaacgtc atcctcggca aagacggacg ggtggtggtg ctcgacttcg
     2041 gcctggccgt cgcgcccgag gtgggcgtcc cgggtccgga ggcggaggcg gcgccggcgc
     2101 cgcccgtgaa cacgacctcc gactcg
//
LOCUS       DASVOR010001281         1803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_392550,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001281 DASVOR010000000
VERSION     DASVOR010001281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392550"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            404..>1803
                     /locus_tag="VF516_24510"
     CDS             404..>1803
                     /locus_tag="VF516_24510"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110927.1"
                     /translation="MVDVGAAPIDRYVVLEHLSEGGMGAIYVGKKLGAGGFEMQVVLK
                     QLLPEFTQQEEFIDLFLREAKLSATLDHANIVHTIDLVTAGGEYFIVMEYLPGGDLRT
                     LLKKAKRRGKRFSPAAAIHISREVLSALAYAHVKRDLDGIPLRLIHRDVSPSNILVSY
                     AGEVKLTDFGIAKAATHNSLFYKVKGKIGYMSPEQARSEQLDHRSDLYSLAVCMYEVL
                     TGERLFVHAGLTTPAEEIYSQPVPQVSRKVPGLTPDLDPIMRKALALSPDDRFQTAGE
                     FQEALTRCAHRNGLLVSAPEVARELVDLCGPPDQWRGDEDDDDLGYVQRAATEVYDGG
                     GEDDEDEALPTSHSGPFSISRHSSSRISRPRTELAKGGVVELTSIINMIDLEQQHREA
                     MGDRSFVGSVSDRFDSSGLRRFDLGPGLAPQVEVQDGRTISGPPPIPRQAPGGAAMPP
                     SQLRPMPLPAMPPTPLP"
BASE COUNT          282 a          621 c          636 g          264 t
ORIGIN      
        1 gtcgcgaaac gtcgaccctt gacggcgacg tcaacgtcga gcccaccgtc gacgttgaac
       61 gtcgaccgac aatggctggt ggaatggtca acggcgctcc ccggcggtca agctctgggt
      121 caacgagggg ctctacgtct acgttgccgt caagcacaac gtcgggggtc aaggtcgagg
      181 tcaaggtcaa gatcttccag ggcgagggga ccggacggcc ccgccccgag gttcccgaac
      241 ggattcgggt ccgccggggc gcagtgccac agccggcggg gccgggagcg tcgtgtccgt
      301 cgccgcggtg ggagcggatg cgtcgggcgc accgctgcgg ccgaacggcg cacatgtccc
      361 cggcccggcg ccgcggggcg gtcgatgtta cactgatcac tcgatggtcg atgtcggagc
      421 agctccgatc gatcgctatg tcgtcctcga gcacctgtcc gagggcggca tgggggcgat
      481 ctacgtcggc aagaagctcg gcgccggcgg cttcgagatg caggtcgtgc tgaagcagct
      541 cctgccggag ttcacccagc aggaggagtt catcgacctg ttcctccgcg aggccaagct
      601 gtcggcgacg ctcgatcacg cgaacatcgt ccacaccatc gacctggtca ccgccggcgg
      661 cgagtacttc atcgtgatgg agtacctgcc gggcggcgat ctgcgcacgc tgctcaagaa
      721 ggccaagcgg cgcggcaagc ggttctcgcc ggccgcggcg atccacatca gccgcgaggt
      781 gctgtccgcg ctggcttacg cccacgtcaa gcgcgacctc gacggcatcc cgctccggct
      841 gatccaccgc gacgtgtcgc cgtcgaacat cctggtgtcg tatgccggcg aggtcaagct
      901 gaccgacttc gggatcgcca aggcggcgac ccacaactcg ctgttctaca aggtcaaggg
      961 caagatcggc tacatgtcgc ccgagcaggc gcggagcgag cagctcgatc accgctccga
     1021 cctgtactcg ctcgcggtgt gcatgtacga ggtgctcacc ggcgagcggc tgttcgtcca
     1081 cgccggcctc accacgccgg ccgaggagat ctactcgcag ccggtgccgc aggtgtcgcg
     1141 caaggtgccg gggctgaccc ccgatctcga cccgatcatg cgcaaggcgc tcgcgctgtc
     1201 gcccgacgac cggttccaga ccgcgggaga gttccaggag gcgctgaccc gctgcgcgca
     1261 ccgcaacggc ctgctggtct cggcgcccga ggtcgcgcgc gagctggtcg acctgtgcgg
     1321 cccgcccgat cagtggcggg gcgacgaaga cgacgatgac ctgggctacg tccagcgcgc
     1381 cgcgaccgag gtctacgacg gcggcggcga ggacgacgag gacgaggcgc tgccgaccag
     1441 ccactcgggg ccgttctcga tcagccggca ctcgagctcg cggatctcgc ggccgcggac
     1501 cgagctcgcc aagggcggcg tcgtcgagct gacgtcgatc atcaacatga tcgacctgga
     1561 gcagcagcac cgcgaggcga tgggcgaccg ctcgtttgtc ggctcggtct ccgatcggtt
     1621 cgactcctcc gggctgcggc ggttcgacct cgggccgggg ctggcgcctc aggtcgaggt
     1681 gcaggacggg cggacgatct cggggccgcc gccgatcccg cggcaggcgc ccgggggagc
     1741 ggcgatgccg ccgtcacagc tccggcccat gccgctgccg gcgatgccac cgacgccgct
     1801 gcc
//
LOCUS       DASVOR010001282         4742 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_42541,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001282 DASVOR010000000
VERSION     DASVOR010001282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4742
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42541"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..767)
                     /locus_tag="VF516_24515"
     CDS             complement(195..767)
                     /locus_tag="VF516_24515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110928.1"
                     /translation="MLPCSPEAVIPVVALVNLLFGIGFALIARDRIRADGPFAAPAFP
                     LVALHAAAVVAPVALYFYAVHPAWSWMYLVNPRKLAGVAVLPLMVGHGVLVIGGWYGA
                     ALLLRRGYLGALLYVAGAIALLLLILVVGGIQRLATATDYPGWQARQGTSLFAVQLGW
                     AFVVSLMALFGSAIYVAIELGRDGRRVRSR"
     gene            complement(816..2582)
                     /locus_tag="VF516_24520"
     CDS             complement(816..2582)
                     /locus_tag="VF516_24520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8110929.1"
                     /translation="MPRVLLTGFCAVPGPRRAGVQLRHVIRALTSLHSVDLLVAREGD
                     QAYVERQGSVRVLRVPTHDGDPRSQIQAFQRALKRQLDGADYDVVHCRDSWSGIPVLE
                     ARSRLGYAMVYDLARSPLGETTLDAELDAQYARDEEACLIAADLVLAPTPAAVKALQG
                     RGKPERVVLSPLGVDVDRFDWDDPPTGGPPRILYVGTIDPGRGVRVLVRAMAAIVREV
                     DARLVLAGSMAPRFEQPLRDQVRELGLADKIEILPPVDHDQLPALLATATVCAVPAAA
                     DLTPSPTVVFPTKLLEYMACRRAVVAPRRETLGHVVENNREALLFEPGDPIDLARKVL
                     RLLGEPLLRDRIAHNAYERVRREFTASAARRALRSAYRVLSERFAVPLHDPAEDDDAP
                     KIELLSDDDFEATVFEQAPAPPPVDTALNKLHPLEEALQSLVESDGTGTTSSERLIAP
                     PPLREPDETMERAPVARPRDASGWTVSALAAPPLPTGHDDWVVTNVANAVRVVEADDL
                     EAELLIEDGTPIEGVVAVAPPLPMVETSFVAGEIDVPTPPPAPERDHDTPAVEFTAAG
                     PILGTPDADPDTGSQTPPMERR"
     gene            2725..2979
                     /locus_tag="VF516_24525"
     CDS             2725..2979
                     /locus_tag="VF516_24525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110930.1"
                     /translation="MHQLIDYLRQHLMIDFQGELTVGRLRELLAGDDSRDAKVLMAKL
                     VAENSVEDMMLVLADCLLEHVQRSLTDDVVREQIRMYAES"
     gene            3121..3399
                     /locus_tag="VF516_24530"
     CDS             3121..3399
                     /locus_tag="VF516_24530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110931.1"
                     /translation="MGNPVPDTKTPALVAFVMVGVGIAIAAVQGIVHGSIAGGVIAAA
                     GAIPACFGMWKGIQQETQGTLALSVTAVLVALAVGAALIVLRVVSWLH"
     gene            3497..4594
                     /locus_tag="VF516_24535"
     CDS             3497..4594
                     /locus_tag="VF516_24535"
                     /inference="COORDINATES: protein motif:HMM:NF014477.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEX8110932.1"
                     /translation="MALGLGAAAGLAAGPMTGRAHADPAAPVTPAVPVEPPHPPDATH
                     PATKIHYASQAMGTNIEVWLWTADERAAAQAAEAVFDEMKRLDLEMSNWKPASDVSQI
                     NAAAGARPVKVSDETYAVIARAVDVSKRSSGVFDITIEAFRGVWKFDQDMDGSLPDPA
                     EVKKRLALVNWKDIALDPRQHSVFLRRKGMAITLGGIAKGYAVDRCVAILRARGFTDF
                     MMQAGGDMYIAGRKGNEPWVVGIRDPRGPTNTMFAVAPIEDHSFSTSGDYERGFVKDG
                     VRYHHIIDTRTGQPAHASRSVTVRARDAFTADAWSKVMFILGPRDALELIRREKLTDF
                     EVVWVDDKNQVVMTDGLKGTIQMLREPTPGP"
BASE COUNT          701 a         1688 c         1742 g          611 t
ORIGIN      
        1 cgtccaccag tacggggcgt acttctcggc ctggaccgcg cgggcgtcct cgaccgcagc
       61 cgccgcgcgg ccgacgtcgc cgacgtagcg gatcggtccg cagccgagcg ccgcgagccc
      121 gagcacggcg accaggggca aggcccggcc tcggcacacg cggcggcaca tcgcgcgcga
      181 gcttaccacc gcggtcaccg cgaccgcacg cggcggccat cgcggccgag ctcgatcgcg
      241 acgtagatcg cagagccgaa cagcgccatc agcgacacca cgaacgccca gcccagctgc
      301 acggcgaaca gcgaggtgcc ctgcctcgcc tgccagccgg gatagtcggt ggcggtcgcc
      361 agccgctgga tcccgccgac caccaggatc agcaagagca gcgcgatcgc accggcgacg
      421 tagagcagcg ccccgaggta gccgcggcgc agcagcaggg cggccccgta ccagccgccg
      481 atcaccagca cgccgtggcc gaccatcagc ggcagcaccg cgacgccggc gagcttgcgc
      541 gggttgacca ggtacatcca gctccacgcc gggtgcaccg cgtagaagta cagcgcgacc
      601 ggggcgacca ccgccgcggc gtgcagcgcg accagcggga acgccggcgc cgcgaacggc
      661 ccatcggcgc ggatccggtc gcgggcgatc agcgcgaacc cgatgccgaa cagcaggttg
      721 accagcgcaa cgaccggaat gacggcctcc ggcgaacacg gtaacacggc ggctgcccca
      781 agctggaacc cgggcgcgcc ggcgggcaat tcacttcacc ttcgctccat cggcggggtc
      841 tgcgagccgg tgtcggggtc ggcgtcgggc gtaccgagga tcgggcccgc ggcggtgaac
      901 tcgaccgccg gggtgtcgtg gtcgcgctcg ggggccggcg gcggggtcgg cacgtcgatc
      961 tcgccggcga cgaacgaggt ctcgaccatc ggcaacggcg gcgccaccgc caccacgccc
     1021 tcgatcgggg tgccgtcctc gatcagcagc tcggcctcga ggtcgtcggc ctcgaccacg
     1081 cggaccgcgt tggcgacgtt ggtcaccacc cagtcgtcgt ggccggtcgg cagcgggggc
     1141 gcggcgagcg ccgacaccgt ccagccgctg gcgtcgcgcg gccgggcgac cggcgcccgc
     1201 tccatggtct cgtcgggctc gcgcagcggc ggcggcgcga tcaaccgctc gctgctcgtg
     1261 gtgccggtgc cgtcgctctc gaccagcgac tgcagcgcct cctccagcgg gtgcagcttg
     1321 ttgagcgcgg tgtcgaccgg cggcggggcc ggcgcctgct cgaacaccgt ggcctcgaag
     1381 tcgtcgtcgg acagcagctc gatcttgggg gcgtcgtcgt cctcggcggg gtcgtgcagc
     1441 ggcaccgcga accgctcgct cagcacgcgg tacgccgagc gcagcgcgcg gcgggccgcg
     1501 ctcgccgtga actcgcggcg cacccgctcg taggcgttgt gcgcgatccg gtcgcgcagc
     1561 agcggctcgc cgagcagccg cagcaccttg cgcgccaggt cgatcggatc gccgggctcg
     1621 aacagcagcg cctcgcggtt gttctcgacc acgtggccca gggtctcgcg gcgcggcgcg
     1681 accacggcgc ggcggcacgc catgtactcg agcagcttgg tcgggaacac cacggtcggg
     1741 ctcggcgtga gatcggccgc ggcgggcacc gcgcacaccg tcgcggtcgc gagcagcgcc
     1801 ggcagctggt cgtgatcgac cggcggcagg atctcgatct tgtcggccag gccgagctcg
     1861 cggacctggt cgcgcagcgg ctgctcgaac cgcggcgcca tcgagccggc gagcacgagc
     1921 cgggcgtcga cctcgcggac gatcgcggcc atcgcgcgga ccagcacgcg cacgccgcgg
     1981 ccggggtcga tcgtcccgac gtagaggatg cgcggcgggc cgccggtcgg cggatcgtcc
     2041 cagtcgaacc gatcgacgtc gaccccgagc ggcgacagca cgacgcgctc gggcttgccg
     2101 cggccctgca gcgccttgac cgccgccggg gtcggcgcga gcacgaggtc ggcggcgatc
     2161 aggcaggcct cctcgtcgcg cgcgtactgc gcgtcgagct ccgcgtcgag cgtggtctcg
     2221 ccgagcggcg accgcgcgag gtcgtagacc atggcgtagc ccagccgcga ccgggcctcg
     2281 agcaccggga tgccgctcca gctgtcgcgg cagtgcacga cgtcgtagtc ggcgccgtcg
     2341 agctggcgct tgagggcgcg ctggaacgcc tggatctgcg accgcgggtc gccatcgtgg
     2401 gtcggcacgc gcagcacgcg gaccgagccc tggcgctcga cataggcctg gtcgccctcg
     2461 cgcgcgacca gcaggtcgac cgagtgcagc gaggtcagcg cgcggatcac atggcggagc
     2521 tggactcccg cgcgccgcgg ccccggcacc gcacagaaac cggtcagcaa cacccggggc
     2581 atggcggagc gatcctcggg caactgacgg acgcgcgcaa ggccgaaccg tcgcggccac
     2641 ggaacggttc ggtgcggcga gatggcttca tcgtcgtcgg tcggccgcct tgatccggat
     2701 cggcggctgc gttatccccg aagcgtgcat cagttgatcg actacctccg gcaacatctg
     2761 atgatcgact tccagggtga gctgacggtc ggcaggctgc gggagttgct cgcgggcgac
     2821 gacagccgcg acgccaaggt gctgatggcc aagctcgtcg ccgagaacag cgtcgaggac
     2881 atgatgctgg tgctcgcgga ctgcctgctg gagcatgtgc agcgctcgct caccgatgac
     2941 gtggtgcgcg agcagatacg gatgtacgcc gagagctagc tgtaaccccg gtcacggcgg
     3001 gatcgtggct gcacggcagg ccgcgcgtgc accgcgcgat gccggtcgta ccggtgcgtc
     3061 cgcgttcgcc ccgtgccgca gattgcgctt catggagtgc cctgtgctac ggtctgttgc
     3121 atggggaacc cggtgcccga caccaagaca cctgcgctcg tggccttcgt catggtcggg
     3181 gtcgggatcg cgatcgcggc ggtgcagggc atcgtccatg gatcgatcgc gggcggggtg
     3241 atcgccgcgg ccggcgcgat ccccgcctgc ttcgggatgt ggaagggcat ccagcaggag
     3301 acccagggca cgctcgcgct cagcgtcact gccgtgctgg tcgcgctcgc ggtcggcgcc
     3361 gcgctgatcg tgctgcgcgt ggtgtcctgg ctgcactgaa cggcaccgaa cagtaccggg
     3421 gaccgtaccg aacgatggtg atgtgacgcc aatgaccgtg ggcccggcga tgcggcgaac
     3481 gggatggccc gcgatcgtgg cgctcggact cggcgccgcg gcggggctcg ccgccggtcc
     3541 gatgaccggc cgcgcccatg ccgatccggc cgcgccggtc accccggctg tcccggtcga
     3601 accgcctcac ccaccggacg cgacccatcc ggcgacgaag atccactacg ccagccaggc
     3661 gatgggcacc aacatcgagg tctggctgtg gaccgccgac gagcgcgccg ccgcgcaggc
     3721 cgccgaggcc gtgttcgacg agatgaagcg gctcgacctc gagatgtcga actggaagcc
     3781 ggcctcggac gtgtcgcaga tcaacgccgc cgccggtgcc cggccggtca aggtctcgga
     3841 cgagacctac gcggtgatcg cacgcgcggt cgatgtgtcg aagcgctcca gcggcgtgtt
     3901 cgacatcacg atcgaggcgt tccggggcgt gtggaagttc gaccaggaca tggacggctc
     3961 gctgcccgac cccgccgagg tcaagaagcg gctcgcgctg gtcaactgga aggacatcgc
     4021 gctcgacccc aggcagcaca gcgtgttcct gcgccgcaag ggcatggcga tcacgctggg
     4081 cggcatcgcc aagggctacg cggtcgaccg ctgcgtcgcg atcctgcgcg cgcgcggctt
     4141 caccgacttc atgatgcagg ccggcggcga catgtacatc gcgggcagga agggcaacga
     4201 gccgtgggtg gtcgggatcc gcgatccgcg cggtccgacc aacacgatgt tcgcggtcgc
     4261 gccgatcgag gaccactcgt tctcgaccag cggcgactac gagcgcggct tcgtcaagga
     4321 cggcgtccgc taccaccaca tcatcgacac ccggaccggt cagccggcgc acgcgtcgcg
     4381 ctcggtgacc gtccgcgcca gggacgcgtt caccgccgac gcgtggtcca aggtgatgtt
     4441 catcctgggc cccagggacg cgctggagct gatccggcgc gagaagctga ccgacttcga
     4501 ggtcgtgtgg gtcgacgaca agaaccaggt cgtgatgacc gacgggctga agggcacgat
     4561 ccagatgctc agggagccga caccgggtcc atgacgccgt cgctggtcgc gccggccggc
     4621 gtccccgcct cggtctccac ctcgatctcg cgcgccgggg cgagccagcg cgcgatcgcg
     4681 cgatcgacgc gggccggggt gaccgcgcgg tggcgggcga gctcggcggc caggccgtcg
     4741 gg
//
LOCUS       DASVOR010001283         3550 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_305458,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001283 DASVOR010000000
VERSION     DASVOR010001283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305458"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF516_24540"
     CDS             <1..255
                     /locus_tag="VF516_24540"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEX8110933.1"
                     /translation="PVCEAVWRLYGEAIARFGAVSTMIERDDDIPELAELVGELDRAR
                     QIAQTAETAETAGTVTGRTVGAAAAVAGSIPGPLREAAGG"
     gene            664..1974
                     /locus_tag="VF516_24545"
     CDS             664..1974
                     /locus_tag="VF516_24545"
                     /inference="COORDINATES: protein motif:HMM:NF013395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEX8110934.1"
                     /translation="MALLPGLLITSALVAATGCATGMPGEGDDGSIEVGTATLSEGFE
                     AGTKTAYAVGDVALTTGTWTLNDALIGDLSSDVKTGTHAARIRNSGTVTMKFDRTTGA
                     GTVTLHHASFGSDASSTWGLFESQNGGSTWAQVGTSRSTTGGSFSTATFTVNLTGTIR
                     FQVRKLDGSANRIDIDDIAITDFTGTGGGGSGAKLSVHTTLGIPAPTSTSTPSAFLSV
                     KSGYVVSYNGSRKVPNWVSWELNTSYLGSTPRQDDFRIDDTFPPSEPQAQLADYSGSG
                     FDRGHMCPSADRTLTVTANSQTFYLTNMVPQAANNNRGPWEALESELRTIAQGGKELF
                     IIAGGVYSSSSTTIGTGVSVPDKTFKVAVVLDAVGQGPANVTTSTRVIAVMMPNSNSL
                     IGLSDPWRNFRVSVDAIEAATGDNFLSDVDPAVQAVIEARVDNQ"
     gene            complement(2033..2185)
                     /locus_tag="VF516_24550"
     CDS             complement(2033..2185)
                     /locus_tag="VF516_24550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110935.1"
                     /translation="MGAGQRANLGEEAQPVPLPGAVTEQEDGVGVADDEGITDDRQTL
                     REQELR"
     gene            2397..>3550
                     /locus_tag="VF516_24555"
     CDS             2397..>3550
                     /locus_tag="VF516_24555"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8110936.1"
                     /translation="MKRAASRDITGPGASVHRVEHTAADGRSVIAVIGIDRYRAWDRL
                     RNAVTDARGVLAAFEDLGFEPVTEPLLDEAATGAAIRRLASDDLRSRLQPDDSLVVFF
                     AGHGHTTVSEFADGGCVKTGYLIPVDAERGDAHRSTWLRLETWLGEIARLPARHILVI
                     LDACHGGIALDCDTRSRGQGARGTDIVDPLRARRSRRVITSALDNERAMDSGPIASHS
                     LFTGYLIEALRGGMVARTGDSLVTGSEIGHYVRRRVRESTCEQQTPDIGTLELDDRGE
                     LLVSLPRPLDAAAPSPASALRESGVAFGPRHGPLRAPGPAPVPRRYGEADAHRTEGWT
                     LDAAFVARLDRHAAERSDGSPVLSVLGGDPMTAQTAWATWAAGHGYLTLVT"
BASE COUNT          552 a         1284 c         1220 g          494 t
ORIGIN      
        1 ccggtgtgcg aggcggtgtg gcggctctac ggcgaggcga tcgcgcggtt cggcgcggtg
       61 tcgacgatga tcgagcgcga cgacgacatc ccggagctcg ccgagctggt cggcgagctc
      121 gaccgggcgc gccagattgc acagaccgca gagaccgcgg agaccgcagg cacggtcacc
      181 ggtcggaccg tcggagccgc ggctgcggtc gccggctcga tcccgggccc gctgcgcgaa
      241 gctgcgggtg gctagtcgag tggccacttc ccaacggagt ggccggtgga gtgggcagcg
      301 gagtggccgg cggcgcggcc gatggctgat ccactagcca cctcgatgtg accggaatcg
      361 ccgacgggcg cggcaagcgc tgcgctcgag gcagcgcttg ctcgacgccg tgcagatcgc
      421 gcacgcggct gtaagccagc gcacaggcga tacacccgcg cactgcgaca gatcacacgg
      481 actgtggcgt gcacgcgggg atcgcgtccc gcatatgtca tacgccctta gagccgattt
      541 catgcaatcg ccaggatcgg cgtgttatcg acgtgcggcc agaaggcaat tccgatcagc
      601 agtcgtggtt tcgagaaagg gtcgatgatg cgcgatacac gatctcggta tctgtccctg
      661 tccttggcgc tgctccctgg gctcctcatc acctcggcgc tggtcgccgc gaccggctgc
      721 gccaccggca tgccgggcga gggcgacgac ggctcgatcg aggtcgggac cgccacgctg
      781 tccgaaggct tcgaggccgg gaccaagacc gcctacgcgg tcggcgacgt cgcgctcacg
      841 accgggacct ggacgctgaa cgacgcgctg atcggcgacc tgagctcgga cgtcaagacc
      901 gggacccacg cggcccggat ccggaacagc ggcacggtca cgatgaagtt cgaccgcacg
      961 accggcgccg gcacggtgac cctccaccac gccagcttcg gcagcgacgc gagcagcacc
     1021 tggggcctgt tcgagtccca gaacggcggc agcacgtggg cccaggtcgg gacctcgcgg
     1081 tcgaccaccg gcggctcgtt ctcgaccgcg acgttcaccg tcaacctcac cggcacgatc
     1141 cggttccagg tccgcaagct cgatggcagc gccaaccgga tcgacatcga cgacatcgcg
     1201 atcaccgact tcaccggcac cggcggtggc ggcagcggcg ccaagctcag cgtgcacacc
     1261 acgctcggca tcccggcgcc gacctcgacc agcaccccca gcgcgttcct gtcggtcaag
     1321 tcgggctacg tggtgtcgta caacggcagc cgcaaggtgc ccaactgggt cagctgggag
     1381 ctcaacacgt cgtacctggg cagcacgccg cgccaggacg acttccggat cgacgacacg
     1441 ttcccgccgt cggagcccca ggcccagctc gccgactaca gcggcagcgg cttcgaccgc
     1501 ggccacatgt gcccgtcggc ggaccgcacg ctgaccgtca ccgccaactc gcagacgttc
     1561 tatctcacca acatggtgcc gcaggccgcc aacaacaacc gcggcccgtg ggaggcgctg
     1621 gagagcgagc tgcgcacgat cgcgcagggc ggcaaggagc tgttcatcat cgcgggcggc
     1681 gtgtacagca gcagctcgac gacgatcggc accggggtct cggtccccga caagacgttc
     1741 aaggtcgccg tggtgctcga cgcggtcggc cagggtccgg ccaacgtcac cacgagcacc
     1801 cgggtgatcg ccgtgatgat gccgaactcg aactcgctga tcggcctgag cgatccgtgg
     1861 cgcaacttcc gggtctcggt cgatgccatc gaggccgcga ccggcgacaa cttcctgtcc
     1921 gacgtcgatc ccgccgtcca ggcggtgatc gaagcgcgcg tcgacaacca gtagtcgcgg
     1981 cgcgtggcag atcgtgggga tggccggcgt gggccagatc gtcctcggtc cgtcaccgca
     2041 gctcctgttc ccgcaaggtc tggcggtcgt cggtgattcc ctcgtcatca gcgacaccaa
     2101 cgccatcctc ctgctccgtc acggcgccag gtagcggcac cggctgcgcc tcctcgccga
     2161 ggttcgcgcg ctggccggca cccacccgcg cgattgacaa ccggctcccg ccgtccacaa
     2221 gacttcctgg ccgcgcgccc gcatgtcacc tactgcacat ccatgcaggg tcgagccgcg
     2281 gcggtctcgt ccggagcgac gagcgtgcgc acggactgca ccgtttcttg tcgtgacctg
     2341 catgcccccg tcgcccaagt ggcgtgacgg ttcggtcgca acgcgcaggt gccccggtga
     2401 agcgagccgc gagccgcgac atcaccggtc caggcgcatc cgtccaccgg gtcgagcaca
     2461 ccgccgccga cgggcggagc gtcatcgccg tgatcgggat cgatcgatat cgcgcgtggg
     2521 accgtctgcg caacgcggtg accgacgcgc gcggcgtgct ggcggcgttt gaagacctcg
     2581 ggttcgagcc ggtgaccgag ccgctgctcg acgaggcggc gaccggcgcc gcgatccgcc
     2641 ggctcgcgtc cgacgatctg aggtcccggc tccagcccga cgacagcctc gtcgtgttct
     2701 tcgccggcca cgggcacacc accgtgagcg agttcgccga cggcggctgc gtgaagaccg
     2761 gctacttgat cccggtcgac gccgagcgcg gcgacgcgca ccgcagcacg tggctgcgcc
     2821 tggagacctg gctcggcgag atcgcacgcc tgccggcgcg gcacatcctc gtcatcctgg
     2881 acgcctgcca cggtggcatc gcgctggact gcgacacgcg ctcgcgcggg cagggtgcac
     2941 gcggcaccga catcgtggac ccgcttcgcg cgcggcggag tcgccgcgtc atcacatcag
     3001 cgctcgacaa cgagcgcgcg atggacagcg gtccgatcgc cagccactcg ctgttcaccg
     3061 gctacctgat cgaggcgctg cgcggcggca tggtcgcgcg aaccggcgac tcgctggtca
     3121 ccggctccga gatcgggcac tacgtccgcc gccgggtccg ggaatcgacc tgcgagcagc
     3181 agacgccgga catcgggacc ctggagctcg acgaccgcgg cgagctgctg gtctcgctgc
     3241 cgagaccgct agatgccgcg gcgccgtcgc ccgcttccgc gctgcgcgag tccggggtgg
     3301 cgttcggccc ccgtcacggg ccattgcgcg caccgggccc agcgccggtt ccgcgccgct
     3361 acggtgaggc agacgcccac cgcaccgagg gctggacgct cgacgccgcg ttcgtggcgc
     3421 ggctcgatcg ccacgccgcc gagcgcagcg acggcagccc ggtgctgtcc gtcctcggtg
     3481 gcgacccgat gacggcacag accgcctggg cgacgtgggc agccggccac ggctacctga
     3541 cgctggtgac
//
LOCUS       DASVOR010001284         1614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_129874,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001284 DASVOR010000000
VERSION     DASVOR010001284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1614
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129874"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VF516_24560"
     CDS             complement(<1..686)
                     /locus_tag="VF516_24560"
                     /inference="COORDINATES: protein motif:HMM:NF013734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110937.1"
                     /translation="MKVLWSWLLELCDLDRQPTAEEGARALTRGGLEIEGMTNLGAGF
                     TGVVVAEVVARRPHPQAEKLTLVDVITERGGAATQVVCGASNVPAPGRKVLWAQVGAT
                     LPGPDGTTLTLGAKPVKGVVSPGMLCAEDELGLGDDHDGIIVLDEDDRTALGAPAQRA
                     LGVDDWLLEVNAPANRGDVLGHLGVARELVAMLRGKLVLPDADLRELRGGDRPALDIA
                     IADAAACPRYT"
     gene            complement(703..>1614)
                     /gene="pheS"
                     /locus_tag="VF516_24565"
     CDS             complement(703..>1614)
                     /gene="pheS"
                     /locus_tag="VF516_24565"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX8110938.1"
                     /translation="KKGKLSEVMKELGRLPAGDRPAVGAAANKVKQAIEDRVAARLGE
                     LADSAGTLDLARTVDVTLPARPVGGGHLHILTQVRLEAIQIFAELGFVVAEGPQIETD
                     WHTFEALAIPKDHPARDMQDTFYVSEEIVLRTHTSPVQVRTMLTQPPPIRIVAPGHVY
                     RRDDDLTHSPMFTQLEGLAVDEGISFADLKGTLLHFVTRFYARDLGIRLRPSYFPFVE
                     PGAEVDMQCTFCMGSGCRLCKGTGWVEIGGAGMVDPEVFGHVNIDAEKYTGFAFGMGF
                     ERMAMLRHGVNDIKFYYEGDVRFLEQF"
BASE COUNT          228 a          578 c          572 g          236 t
ORIGIN      
        1 gtgtagcgcg ggcacgccgc ggcgtcggcg atcgcgatgt ccagcgccgg gcggtcgccg
       61 ccgcgcagct ccctcagatc ggcgtcgggc aggaccagct tgccgcgcag catcgcgacc
      121 agctcgcgcg ccacgccgag gtggccgagc acgtcgccac ggttggccgg cgcgttgacc
      181 tcgagcagcc agtcatcgac cccgagcgcg cgctgggcgg gtgctccgag cgccgtgcga
      241 tcgtcctcgt cgagcacgat gatcccgtcg tgatcgtcgc cgagcccgag ctcgtcctcg
      301 gcgcacagca tgcccggcga cacgacgccc ttcaccggct tggcgccgag cgtgagggtg
      361 gtgccgtccg gccccggcag cgtcgcgccg acctgggccc acagcacctt gcggccgggc
      421 gccgggacgt tgctcgcgcc acacaccacc tgggtcgcgg ccccaccgcg ctcggtgatg
      481 acgtcgacca gggtcagctt ctcggcctgg gggtgcggcc gcctggcgac gacctcggcg
      541 accacgacgc cggtgaaccc ggcgccgagg ttcgtcatgc cctcgatctc caggccgccg
      601 cgggtcagcg cacgcgcgcc ctcctcggcg gtcggctggc gatcgaggtc gcacagctcc
      661 aggagccagc tccacaggac cttcatggcg actcaccccg catcaaaatt gctccaggaa
      721 ccgcacgtca ccctcgtagt agaacttgat gtcgttgacg ccgtggcgga gcatcgccat
      781 gcgctcgaag cccatcccga acgcgaagcc ggtgtacttc tcggcgtcga tgttgacgtg
      841 gccgaatacc tcggggtcga ccatgccggc gccgccgatc tcgacccagc cggtgccctt
      901 gcacagccgg cagccgctgc ccatgcagaa cgtacactgc atgtcgacct cggcgcccgg
      961 ctcgacgaac gggaagtagc tcggccggag ccggatcccg aggtcgcggg cgtagaaccg
     1021 ggtcacgaag tggagcagcg tgcccttgag gtcggcgaac gagatgccct cgtcgaccgc
     1081 gagcccctcg agctgggtga acatcggcga gtgggtcagg tcgtcgtcgc ggcggtagac
     1141 gtggccgggc gcgacgatcc ggatcggcgg cggctgggtc agcatggtgc ggacctggac
     1201 cggcgaggtg tgggtccgca gcacgatctc ctcggacacg tagaacgtgt cctgcatgtc
     1261 gcgcgcgggg tgatccttgg ggatcgcgag cgcctcgaag gtgtgccagt cggtctcgat
     1321 ctgcggacct tcggcgacga cgaagccgag ctcggcgaag atctggatcg cctccaggcg
     1381 gacctgggtc aagatgtgga ggtggccgcc gccgaccggg cgcgccggca gggtgacgtc
     1441 gaccgtgcgc gcgaggtcga gcgtgccggc cgaatcggcg agctcgccga gccgcgccgc
     1501 gacccggtcc tcgatcgcct gcttgacctt gttggccgcg gcgccgaccg ccggccgatc
     1561 gcccgccggc agccggccga gctccttcat gacctcggac agcttgccct tctt
//
LOCUS       DASVOR010001285         5890 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_451364,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001285 DASVOR010000000
VERSION     DASVOR010001285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5890
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451364"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3894)
                     /locus_tag="VF516_24570"
     CDS             complement(<1..3894)
                     /locus_tag="VF516_24570"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEX8110939.1"
                     /translation="MSSERRPTAGRAQPEGRSPTTAGSAQEPVARGHGAATSEQTGAG
                     ARAQSLLPSITLPRGGGAIRGIGEKFSTNPATGTASLSIPIATSPGRAGFELGLALTY
                     DSGAGNGPFGLGWHLSTPSITRKTDKGLPRYLDDVESDVFVLSGAEDLVPERLGDGDL
                     PPFGRGDHSVQRYRPRVEGLFARIERWTNRATGDAHWRAITRDNVLNIYGRTPAARIA
                     DPAHPERVFSWLLEETRDDRGNIARYTYKPEDDRNVDPARASESNRFETQGGTRVFHA
                     TAQRYLERIQYGNRQPVMDRESPAPDGSDDYLFEVVFDHGEYAGPAADRPELAPDPGV
                     PGDWPTRQDPFSAFRSGFEVRTYRLCRRVLMFHRFPGEASLGITPCLVRSTDLAYDEG
                     PVVTYLRSVTQAGYVWDQATGEYRRAALPPLELGYVKPEVHDELRSIDRASLEGISSG
                     IEGSGGQWADLDGEGISGVLLPSERAWYYKPNLGEGQLAPPELQRQLPSPAELGGGMQ
                     QLSDLGGDGNLDLVRYAPPLSGYFERTPEGTWASHVALHQVPNLDWSDPNLRFLDVDG
                     DGLPDILISEHDAFVWYRSRGKEGFEPAALAAKPKNEQQGAAIVFADGTETIQLADMS
                     GDGLVDIVRVRNGEVCYWPNLGHGRFGRKVTLDGGPRFDAPDRFDPRRVRLADIDGSG
                     TSDILYLGRDGARLYFNQSGNGLAEARRLASLPPIDAASTVSVVDLLGHGTACLVWSS
                     ALPAHRAQPVVYVDLMGGKKPHLLASVTNNFGAETRIAYAPSTRFYLDDKQAGRPWLT
                     RLAFPVHVIERIERVDHVAKSRLVTRFAYHHGYFDGHEREFRGFARVEQWDTETFSGD
                     KGKGLFPELPYDVDPADPDLNLPPVRTVTWFHTGAWLERERLERALAAEYYGQDPQAP
                     LLPDTTLPPGLSIREQREAARALRGQILRQEIYAEDPVPEAAHPYTVSERSYEVRRIQ
                     AADGASHGVFFVHPSQTITLHYERRPADPRMQQEVVLAVDDFGQVTQSAVLAYPRRLP
                     ERQREEATTGRRLDEQSRMWATLTERGFADRPAEADWYRASVPTWTRTSELTGLPAPA
                     QGPLAPEALRGAISGATEIPFHDEPTGTGVEKRLIDCEERFYYGGHATVADYRSDALG
                     LPIGEITERALVHHVERFAFTEPFLARSYAGAVDAALLASAGYTQKDNTWWVPSGRAA
                     YDPARFFLPVEAVDPFGQWTAVEYDVHCVLAVASKEPRVAGDALSGQVDTADQRFGSV
                     TRSEQDYRVLAPSAIADSNRNRTRVVFNALGLPEEI"
     gene            4447..5157
                     /locus_tag="VF516_24575"
     CDS             4447..5157
                     /locus_tag="VF516_24575"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX8110940.1"
                     /translation="MTTVNKPAMQRHTVFLVQILWLLTLGVLAGLYFYTDVFGDRPLG
                     PIPLGVIWFGALGAILISLNGIVDHAHDWDPSFDLWHLSRPVMGAALAVVGVLMMQAG
                     VLAVGREPGNGTIPQNLLYYLVAFLIGYREETFRELMKRLVDLILAPATSKPAPSIAK
                     LDPSSAPKAGKRPVVITGAGLSGATSVVFGSQAAEFHVDSDNRVTAELPVTDAAGPVA
                     VVVKTSAGAASHTFDYAA"
     gene            5217..>5890
                     /locus_tag="VF516_24580"
     CDS             5217..>5890
                     /locus_tag="VF516_24580"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8110941.1"
                     /translation="MLKRGDQGDEVRKLQEILLALGYQLPRWGADGQLGNETIDAFAR
                     FLRDHSTEVDDDAGVVSDAELALVQKVFAATRDAPLGPKLASGRFHDLRTIASQDQIG
                     GRRPWKQITGITLHQTACVLGEKPRRWATVGAHLGVTRAGQVIWMHDFEQIVWHANGF
                     NARTVGIEMDGTYAGIEGDVRTWWKDPDAPDSRPQTPTIELVEAAKATVRWICDEVVR
                     HGGRIE"
BASE COUNT          920 a         1928 c         2119 g          923 t
ORIGIN      
        1 gatctcctcg ggcaacccga gcgcgttgaa cacgacgcgc gtgcggttgc ggtttgaatc
       61 ggcgatcgcc gagggcgcga gcacgcggta gtcctgctcg gaccgggtga cgctgccgaa
      121 ccgctgatcg gcggtgtcca cttgtccgga caaggcatca ccggcgacgc gaggctcctt
      181 gctggccacc gcgagcacgc agtggacgtc gtattcgacc gcggtccact ggccgaaggg
      241 atcgactgcc tcgacgggca ggaagaagcg agcggggtcg taggctgccc ggccggaggg
      301 aacccaccag gtgttgtcct tctgcgtgta gccggcgctc gcgagcagcg cggcgtcgac
      361 cgcacctgcg taggatcgtg cgaggaaggg ctcggtgaag gcgaagcgct cgacatggtg
      421 cacgagtgcc cgctcggtga tctctccgat cggcagcccg agcgcatcag atcggtaatc
      481 ggcgacggtg gcgtggccgc cgtaatagaa ccgctcctcg caatcgatga ggcgcttctc
      541 gacgccggtc ccggtcggct cgtcgtggaa cggaatctcg gtggcgccgg agatggcgcc
      601 tctcagcgcc tccggcgcca gcggcccctg cgccggtgcc gggaggccgg tcagctcgga
      661 ggtcctcgtc caggtgggca cgctcgcgcg ataccagtcc gcttcggccg gccggtccgc
      721 gaagccgcgc tccgtcagcg tcgcccacat gcggctctgc tcgtcgagcc ggcggcctgt
      781 ggtcgcctcc tctcgctgtc gctccggcag ccgccgcgga taggcgagca cggccgactg
      841 ggtgacctgt ccgaagtcgt cgacagccag caccacctcc tgctgcatcc gcggatcggc
      901 gggccggcgc tcgtagtgga gcgtgatggt ctggctcggg tgcacgaaga agacgccgtg
      961 cgacgcgccg tcggctgcct ggatccggcg cacctcatag ctgcgctccg agacggtgta
     1021 cggatgcgct gcctcgggga cgggatcttc ggcgtagatc tcctggcgca agatctgacc
     1081 gcgcagcgcg cgagccgcct cgcgctgctc gcggatggag agtcccggcg ggagcgtggt
     1141 gtccggcagc agcggggcct ggggatcctg gccgtagtac tccgcggcga gcgcgcgctc
     1201 gagccgctcg cgctcgagcc acgctccggt gtggaaccag gtgacggtgc ggaccggcgg
     1261 caggttcaga tccgggtcgg cagggtcgac gtcgtacggg agctcgggga acaggccctt
     1321 gcccttgtcg ccggagaacg tctcggtgtc ccactgctcc acgcgcgcga agccgcggaa
     1381 ctcgcgctcg tggccatcga agtatccgtg gtggtaggcg aagcgcgtga cgagcctgga
     1441 cttcgcgacg tgatcgacgc gctcgatccg ctcgatgacg tgcaccggga acgccagccg
     1501 cgtcagccag gggcgcccgg cctgcttgtc gtcgaggtag aaccgggtcg aaggtgcgta
     1561 ggcgatgcgc gtctcggcgc cgaagttgtt cgtcaccgac gcgagcaggt gcggcttctt
     1621 gccgcccatg agatcgacgt agaccacggg ctgcgcgcgg tgggcgggca gggccgaaga
     1681 ccacacgagg cacgcggtgc cgtggccgag caagtcgacg acgctcaccg tcgatgcagc
     1741 gtcgatcggt gggagcgacg cgaggcgcct ggcctcggcc aggccgttgc cggactggtt
     1801 gaagtagagg cgtgcgccat cgcggccgag atagaggatg tcgctcgtgc ccgagccatc
     1861 gatgtcggcc agccggaccc gcctgggatc gaatcgatcc ggcgcgtcga agcgcgggcc
     1921 tccgtccagc gtgaccttgc ggccgaagcg gccgtggccg aggttgggcc agtaacagac
     1981 ctcgccgttg cgcacgcgca cgatgtcgac gaggccgtcg ccgctcatgt cggcgagctg
     2041 gatcgtctcg gtgccgtcgg cgaagacgat cgctgcgccc tgctgctcgt tcttcggctt
     2101 cgcagcgagg gcggcgggct cgaagccttc cttgccgcgg gaccgatacc agacgaaggc
     2161 gtcgtgctcc gagatcagga tatccggaag cccgtcgccg tcgacatcga ggaatcgcag
     2221 gttgggatcg ctccagtcga ggttcgggac ctggtggagc gcgacatggg acgcccacgt
     2281 tccttcgggg gtgcgctcga agtacccgga gagcggcggg gcatagcgaa cgagatcgag
     2341 gttgccgtcg cccccgaggt ccgagagctg ctgcattcca ccgccgagct ccgcggggga
     2401 ggggagctgg cgctggagct ccggcggtgc gagctgcccc tcgccgaggt tcggcttgta
     2461 gtaccaggcg cgctcggagg ggaggaggac gccggagatg ccctcgccgt cgaggtcggc
     2521 ccactggcca cccgagcctt cgatgccgct cgagatgccc tcgaggctcg cgcgatcgat
     2581 cgacctcagc tcgtcgtgga cctcaggctt gacatacccg agctcgagcg gcgggagcgc
     2641 cgcgcgccgg tactcgccgg tggcctggtc ccagacgtat cccgcctgcg tgaccgaccg
     2701 gagataggtg acgaccgggc cttcgtcgta ggcaagatcg gtcgagcgca ccaggcaggg
     2761 cgtgatcccc agcgaggcct cgccggggaa ccggtggaac atgagcacgc ggcggcagag
     2821 gcgataggtg cgaacctcga agccggagcg gaacgcggag aatggatcct ggcgagtggg
     2881 ccagtcgccg ggcacgccgg gatccggtgc gagctcgggt cgatcagcag ccgggccggc
     2941 atactcacca tgatcgaaca cgacctcgaa gaggtaatcg tcgctgccgt ccggagccgg
     3001 ggactcccga tccatcacag gctgccggtt gccgtactgg atccgctcca ggtagcgttg
     3061 tgcggttgcg tgaaatacgc gcgtgccgcc ctgcgtctcg aagcgattcg actcgctcgc
     3121 cctcgcggga tcgacgttgc ggtcgtcctc gggcttgtac gtgtagcgcg cgatgttgcc
     3181 gcgatcgtcg cgggtctctt cgagcaacca cgagaagacg cgctccggat gcgcggggtc
     3241 cgcgatccgg gccgcgggcg tcctgccgta gatgttgagc acgttgtcgc gcgtgatcgc
     3301 gcgccagtgg gcgtcgccgg tggcgcggtt cgtccagcgc tcgatgcggg cgaacaggcc
     3361 ctcgacgcga gggcggtagc gctgcacgct gtggtcgccg cgaccgaagg ggggcaggtc
     3421 gccatcgccg agccgctcgg gcacgagatc ctcggcgccg gagagcacga acacgtcgga
     3481 ctcgacgtcg tcgaggtagc gggggagccc cttgtcggtc ttgcgcgtga tcgacggcgt
     3541 cgacaggtgc cagccgaggc cgaacgggcc gttgccggcg cccgagtcgt acgtcagggc
     3601 caggccgagc tcgaacccgg cgcggccggg ggacgtggcg atgggaatgc tgagcgaggc
     3661 ggtgcccgtc gccgggttgg tcgagaactt ctcgccgatg ccgcggatgg cgccacctcc
     3721 cctgggaagg gtgatggacg ggagcaggct ctgcgcgcgc gctccggcgc ctgtctgctc
     3781 ggaggtggcc gcgccatggc cacgagcgac tggctcctga gcagaccctg ccgtggtcgg
     3841 gctgcgacct tcgggctgcg cccggcccgc ggtcggcctt cgctccgagc tcacggcgcc
     3901 cccggtcgaa accaggttga ttcgccgacg tcaacaacgg tgttgccgtg accgatcgat
     3961 tcccttcgca tcgcgaacga ccatcaagca agtggccggc cagcgcgggc gatacgccgc
     4021 aggctcgatc gcacgcatct cttgcgcggc ttcgagaatc tgggtgatca atgcgcgatc
     4081 cgccggcagg gtcctggatc cgttgcctgc gcacgagcgc cgagatagcg cagcaattcg
     4141 cgacgcctgc tcgcaccggg accgctgcgc gtcgtcgcga gaacgtgaac cacgtgcagt
     4201 cttccagggg aagtcgcgca tggactgcag gcgagccgag atgggcagaa gattccagct
     4261 ccatcgcgac acgcaccctg aacatcggcg gcagcacgac agacgcaata atgacaacta
     4321 tgcgaatttg cagtcccgac aggcagctac gagccccaca tcggtccagt ttttgctact
     4381 cgcttcggca tgcccttcat ggggcggggg agccgagtcc cgagctcggc aagataggag
     4441 tgagccatga cgacggtaaa taagccagcg atgcaacgac acacagtctt cctggtccag
     4501 atcctctggc tcctgacgct cggagtcctc gcggggctgt atttctatac ggacgtcttt
     4561 ggcgatcgac cgctcggtcc gattccgctg ggcgttatct ggttcggtgc gctgggcgcc
     4621 attctgatct cgctcaatgg catcgtcgac cacgctcatg actgggaccc gagcttcgat
     4681 ctctggcacc tgtcgcgtcc tgtgatgggc gctgcgctcg cggtcgtcgg cgtcctcatg
     4741 atgcaggccg gcgtcctcgc ggtcggcagg gagccgggca acggcaccat tccgcagaac
     4801 ctgctctact atctggtcgc gttcttgatc ggatatcgcg aggagacgtt ccgggagctc
     4861 atgaagcgcc tggtcgatct gatcctcgcg ccggccacct cgaagccagc gccgtcgatc
     4921 gcgaagctcg acccgtccag cgcgccgaag gccggcaaga ggccggtcgt gatcaccggg
     4981 gctgggctgt ccggggcaac ctccgtggtg ttcggctccc aggcggccga atttcatgtc
     5041 gattcggaca accgcgtgac cgcggagctg cccgtgaccg atgctgccgg gcctgtcgcg
     5101 gtcgtcgtca agacctcggc cggtgccgcc agccacacgt tcgactacgc cgcctgagca
     5161 acgaccaccg cacaggaccg cgacgcacac gtggcgtgcg tccgaggagg agcatcatgc
     5221 tgaaacgagg cgatcagggt gacgaggtcc gcaagctgca agagattctt ctggcgctcg
     5281 gttaccagct gccgcgctgg ggggccgacg gccagctcgg caacgagacc atcgatgcgt
     5341 tcgcgcgctt cctgcgcgat cacagcacgg aggtcgacga cgatgccggc gtggtctccg
     5401 atgcagagct cgcgctcgtg cagaaggtgt tcgcggcgac ccgggacgcg ccgctgggcc
     5461 ccaagctcgc gagcggcagg ttccacgatc tgcgcacgat cgcgagccag gaccagatcg
     5521 ggggccggcg gccgtggaag cagatcaccg gcatcaccct gcatcagacc gcgtgcgtgc
     5581 tcggggagaa gccgcgccgc tgggcaacgg tcggcgcgca cctgggcgtg acccgcgccg
     5641 gccaggtgat ctggatgcac gacttcgaac agatcgtgtg gcacgccaac ggcttcaacg
     5701 cccgcaccgt cggcatcgag atggacggta cctacgccgg gatcgaaggc gacgtccgca
     5761 cgtggtggaa ggacccggac gccccggatt ccaggccaca gacgccgacg atcgagctcg
     5821 tcgaggccgc gaaggcgacg gtgcgctgga tctgcgacga ggtcgtgcgc cacggcgggc
     5881 gcatcgagca
//
LOCUS       DASVOR010001286         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_13928,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001286 DASVOR010000000
VERSION     DASVOR010001286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13928"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1052
                     /locus_tag="VF516_24585"
     CDS             <1..1052
                     /locus_tag="VF516_24585"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEX8110942.1"
                     /translation="YHPGHDYNVDRMIRGMKASLALFSEQFSPFQFHQARILEFPSYA
                     MFAQSFANTIPYSEDIGFLLQLDDPSKIDVATYVTAHEIAHQWWGHQLVPSDQQGASM
                     LVESFAQYSALLVMERMYGKEQIRKFLKYALDNYLRSRGGEVVEELPLARVEEQPYLH
                     YHKGSLVMYWLKEVVGEPRLDGALAALLREYAFKAAPYPNTRDFLRLLRAAAGPEYDS
                     LITDLFERITLVDARATAATATGRADGSYQVQIDVEAHKFYADGKGVETEAPLDEPFD
                     IGVFTAEPGKPGFTAASVLSMKHEPIQSGKQTVTVIVSQRPAWAGIDPYNKRIDRNSD
                     DNLVKVEVRAAAPAR"
     gene            complement(1082..>1676)
                     /locus_tag="VF516_24590"
     CDS             complement(1082..>1676)
                     /locus_tag="VF516_24590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110943.1"
                     /translation="SVLFAVMELDRLSGPVFPALQALHADQRVFSYGISDTPRGIQLY
                     APGKKTGVLVTGKPVGTQLPPPFCQVPSLGFDHQIHHKFIVCGFNGADPVVYCGSSNL
                     ADSSETQNGDNLLAIHDRSVALVFAIEALGLVDHFDFLDRCATRKPGDRAAAHTAPAS
                     KRRAAAEAGWFLSTTDQWARPYFDPRDLRCADRILFR"
BASE COUNT          285 a          587 c          555 g          249 t
ORIGIN      
        1 actaccaccc gggccacgac tacaacgtcg atcgcatgat ccggggcatg aaggcgtcgc
       61 tcgcgctgtt cagcgagcag ttctcgccgt tccagttcca ccaggcgcgg atcctcgagt
      121 tcccgtccta cgcgatgttc gcccagtcgt tcgccaacac catcccgtac agcgaggaca
      181 tcggcttcct gctccagctc gacgatccga gcaagatcga cgtcgcgacc tacgtcaccg
      241 cccacgagat cgcgcaccag tggtggggcc accagctggt gccgagcgat cagcagggtg
      301 cgtcgatgct ggtcgagagc ttcgcgcagt actccgcgct gctggtgatg gagcggatgt
      361 acggcaagga gcagatccgc aagttcctca agtacgcgct cgacaactac ctgcgcagcc
      421 gcggcggcga ggtcgtcgag gagctgccgc tcgcgcgggt cgaggagcaa ccgtacctcc
      481 actaccacaa gggctcgctg gtgatgtact ggctcaagga ggtggtgggc gagcccaggc
      541 tcgacggcgc gctcgccgcg ctgctccgcg agtacgcgtt caaggccgcg ccgtacccca
      601 acacccgcga cttcctgcgc cttttgcgcg cggccgccgg cccggagtac gactcgctga
      661 tcaccgacct gttcgagcgc atcacgctgg tcgacgcccg ggccaccgcc gcgaccgcga
      721 ccggacgcgc cgacggcagc taccaggtcc agatcgacgt cgaggcccac aagttctacg
      781 ccgacggcaa gggcgtcgag accgaggcgc cgctcgacga gccgttcgac atcggcgtgt
      841 tcaccgccga gcccggcaag cccgggttca ccgccgcgtc ggtgctgtcg atgaagcacg
      901 agccgatcca gtccggcaag cagaccgtga ccgtgatcgt ctcccagcgg cccgcgtggg
      961 ccggcatcga tccctacaac aagcggatcg accgcaactc cgacgacaac ctggtcaagg
     1021 tcgaggttcg cgcggcagcg ccggcgcggt gagtcggcgc catgctgcca ccgccgggcg
     1081 gtcagcggaa caggatgcgg tcggcgcaac gcagatcgcg cgggtcgaag tacggcctgg
     1141 cccactgatc ggtggtcgac aggaaccacc cggcctcggc cgctgcccgc cgcttgctcg
     1201 ccggcgccgt gtgcgctgct gcgcggtccc cgggcttccg cgtggcgcac cgatcgagga
     1261 agtcgaagtg gtcgaccagg cccagcgcct cgatcgcgaa caccagggcg acgctgcgat
     1321 catggatcgc cagcaggttg tcgccgttct gcgtctcgct gctgtccgcc aggttggacg
     1381 agccgcagta caccacggga tccgcgccgt tgaatccgca cacgatgaac ttgtggtgga
     1441 tctggtgatc gaacccgagc gacgggacct ggcagaacgg cggcgggagc tgggtgccga
     1501 ccggcttgcc ggtgaccagc acaccggtct tcttgccggg cgcgtagagc tggatcccgc
     1561 gcggcgtatc ggagatgccg tagctgaaca cccgctgatc cgcgtgcagc gcctggagcg
     1621 cgggaaacac cggtcccgat aaccggtcga gctccatcac cgcgaacagc acgctg
//
LOCUS       DASVOR010001287         1720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_392607,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001287 DASVOR010000000
VERSION     DASVOR010001287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_392607"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /locus_tag="VF516_24595"
     CDS             <1..1165
                     /locus_tag="VF516_24595"
                     /inference="COORDINATES: protein motif:HMM:NF041621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MXAN_5187 C-terminal domain-containing protein"
                     /protein_id="HEX8110944.1"
                     /translation="ASGNLLAMNEVRTVTAKQWKTDKGESIIPALNVVLGKKLIISDL
                     WNQGGDMMKIGVAPVIDPDAPVSAQDPDGQVIIGAIVVAYAQTAKLAQQDRNLLGTEI
                     AYYDNRNVMATSFTTAAGAEDTAKAHALTELLHSGTLTEGGAKQKIAVDGAEYLAAAV
                     KMPRSSTRRLPADYPPVTAGAVVLAPVADPSNLAGTVKLFILLLGGGALAIALVGLYL
                     SHRRLIAQVDQIELGVTDIINGNVDRTFRPVGQELDGLANGLNVMLARLLGRPEPGEE
                     EFDDEGNPILQGRVEFEDGDGAGTGAAPAADLDLAALAQESEPDYYKRVYTEYLAARR
                     STGNPDEVSFENFIAKLKVNEGKLKAQYQCRAVRFRVVVNDGKVTLKPVPIFA"
     gene            1175..>1720
                     /locus_tag="VF516_24600"
     CDS             1175..>1720
                     /locus_tag="VF516_24600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEX8110945.1"
                     /translation="MTVQTGLARLAIEGSSLLEGRRLGLLVNATAVDAQLRHAIDLLR
                     ARSDVKVTALFGPEHGVRGDAQDMIAVDGARDARSDLPVHSLYGTTAASLSPTPQMLD
                     AIDVMVYDIQDVGSRYYTFVWTMVLAMRACAAAGKAFCVLDRPNPIDGLHLEGPLIAP
                     GFESFVGLVSCPVRHGLTAGEI"
BASE COUNT          279 a          607 c          599 g          235 t
ORIGIN      
        1 cgccagcggc aacctgctcg cgatgaacga ggtccgcacg gtcaccgcca agcagtggaa
       61 gaccgacaag ggcgagtcga tcatcccggc gctcaacgtc gtgctcggca agaagctgat
      121 catcagcgac ctgtggaacc agggcggtga catgatgaag atcggcgtcg cgccggtgat
      181 cgatccggat gcgccggtgt cggcgcagga ccccgacggc caggtgatca tcggcgcgat
      241 cgtcgtcgcc tacgcgcaga ccgccaagct ggcgcagcag gaccgcaacc tcctgggcac
      301 cgagatcgcg tactacgaca accgcaacgt gatggcgacc agcttcacga cggcagccgg
      361 cgcggaggac accgccaagg cgcacgcgct gaccgagctg ctccactcgg gcaccctgac
      421 cgagggcggc gccaagcaga agatcgcggt cgacggcgcg gagtacctgg cggcggcggt
      481 caagatgccg cgcagctcga cccgccggct gccggccgac tacccgccgg tgaccgccgg
      541 tgcggtggtg ctcgcgccgg tggcggatcc gagcaacctc gcgggcacgg tgaagctgtt
      601 catcctgctg ctcggcggcg gcgcgctggc gatcgcgctg gtcgggctct acctgtcgca
      661 ccgccggctg atcgcccagg tcgaccagat cgagctcggc gtcaccgaca tcatcaacgg
      721 caacgtcgat cgcacgttcc gcccggtcgg ccaggagctc gacggtctgg ccaacgggct
      781 caacgtcatg ctcgcccgcc tgctcggccg gcccgagccc ggcgaggagg agttcgacga
      841 cgagggcaac ccgatcctcc agggccgggt cgagttcgag gacggcgacg gggccggcac
      901 cggcgctgcg ccggcggccg acctggatct cgccgcgctc gcccaggagt cggagcccga
      961 ctactacaag cgggtctaca ccgagtacct ggccgcgcgc cggtcgaccg gcaaccccga
     1021 cgaggtgtcg ttcgagaact tcatcgccaa gctcaaggtc aacgagggca agctcaaggc
     1081 ccagtaccag tgccgcgcgg tgcggttccg cgtcgtggtc aacgacggca aggtcacgct
     1141 caagccggta ccgatcttcg cgtaggctga gcccatgacc gtccaaaccg ggttggcgcg
     1201 actggcgatc gagggcagct cgctgctgga gggcaggcgg ctcggcctgc tggtcaacgc
     1261 gaccgcggtc gatgcccagc tccgccacgc gatcgacctc ttgcgcgcgc gatccgacgt
     1321 gaaggtcacc gcgctgttcg gccccgagca cggcgtgcgc ggcgacgcgc aggacatgat
     1381 cgcggtcgac ggcgcgcgcg acgcgcgcag cgacctgccg gtccacagcc tgtacggtac
     1441 gacggccgcg tcgctgtcgc cgactcccca gatgctcgac gccatcgacg tcatggtcta
     1501 cgacatccag gacgtcggca gccgctacta cacgttcgtg tggacgatgg tgctggcgat
     1561 gcgggcgtgc gcggcggcgg gcaaggcgtt ctgcgtgctc gaccggccca acccgatcga
     1621 cggcctccac ctcgagggcc cgctgatcgc gccgggcttc gagtcgttcg tcggcctggt
     1681 gtcgtgcccg gtccgccatg gcctgaccgc cggcgagatc
//
LOCUS       DASVOR010001288         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_480894,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001288 DASVOR010000000
VERSION     DASVOR010001288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480894"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1819)
                     /locus_tag="VF516_24605"
     CDS             complement(173..1819)
                     /locus_tag="VF516_24605"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEX8110946.1"
                     /translation="MRVGNRISWAGVVVAAVVGCYKESQLPIAPEASLAQHGSVDWRT
                     YNANLAGERFSPIQGLSPRVAPSLRRTCVFDTGEPGPGGETGPIVINGVLYFTHNATT
                     FAVDGATCREKWRHVRKAAPGVTLGNNRGVAFLDGKLFRGYSDGFVLAFDAASGKVLW
                     EVQIVGHVPGEGLDMAPIASNGLVYIGNSGGDIFGVTGHIFALRASDGSIAWRFDTVP
                     NTPEVLATWPEASPQNPPGGGAVWTSFALQAGVLYVTTGNAEPDFQIELRSGQALYTN
                     ALLALDARSGKLLAYVQPIPRDEHDHDVAAGPALITTRGGLHLALAAAKDGRAYGIQL
                     VTLPGGRELAVKYFVATTTRGNLAERLSTERPVRFCPGTNGGTEWNGPAFSPILNLAF
                     VGAVDRCTTIQILTPAQTIAEQGHPGKPYTGGADPNALFGTSDPYAFARGWLTAFDAD
                     TGALRWKYMAPAPVVAGVTVTAGGLLFMGDVSGILRALDARTGTPLWEDHAGNALGGG
                     IVTYEARGRAFLAVVTGQPSGGFPVESPQNIRVVVYSPAR"
BASE COUNT          297 a          742 c          724 g          299 t
ORIGIN      
        1 ctcgcgctcg ccggccggct gtggacgacc gatcgcgccg ccgcgatcga ccacctcgac
       61 gccgccgagg cccggttcac cgtcgcgcgc gccgagcgct ggctgcggcg ggcccaggac
      121 cagcgcggca tcgccgcctg atcggcgccc cgggcatcgc gctcgagctc gctcagcggg
      181 ccggcgagta gacgacgacc cggatgttct gggggctctc gaccgggaag ccgcccgatg
      241 gctggccggt caccaccgcg aggaatgcac ggccacgggc ctcgtaggtc acgatgccgc
      301 cgccgagcgc attgccggcg tggtcctccc agagcggcgt gccggtccgc gcgtccaggg
      361 cgcgcaggat gccggagacg tcgcccatga acaggagccc gccggcggtc acggtcacgc
      421 ccgcgacgac cggggccggg gccatgtact tccagcgcag cgcgccggtg tcggcgtcga
      481 acgcggtgag ccagccgcgc gcgaaggcat acgggtcaga cgttccgaac agcgcattcg
      541 ggtcggcgcc cccggtgtac ggcttgccgg ggtggccttg ctcggcgatc gtctgcgcgg
      601 gggtcaggat ctggatcgtc gtgcaccggt ccacggcgcc gacgaacgcg aggttgagga
      661 tgggcgagaa cgccggcccg ttccactcgg tcccgccgtt ggtgccggga cagaatcgca
      721 ccggtcgctc cgtggacagc cgctccgcga ggttgccgcg ggtcgtcgtg gcgacgaagt
      781 acttgaccgc gagctcgcga ccgccgggca acgtgacgag ctggatgccg tacgcccgcc
      841 cgtccttggc cgccgccagc gcgaggtgaa ggccgccgcg cgtcgtgatc agcgcgggtc
      901 ccgcggccac gtcgtggtcg tgctcgtcgc gtgggatcgg ctgcacgtac gcgagcagct
      961 tcccgctgcg cgcgtcgagc gcgagcaggg cgttggtgta cagggcctgg cccgagcgca
     1021 gctcgatctg gaagtccggc tcggcgttgc cggtcgtcac gtagagcacg ccggcctgca
     1081 gcgcgaacga tgtccagacc gcaccgccgc cgggaggatt ctgcggcgag gcctcgggcc
     1141 aggtcgcgag cacctccggc gtgttgggga cggtgtcgaa ccgccacgcg atcgatccgt
     1201 cgctcgcgcg cagcgcgaag atgtgaccgg tcacgccgaa gatgtcgcca cccgagttgc
     1261 cgatgtagac cagtccgttc gacgcgatcg gcgccatgtc gagcccctca ccggggacat
     1321 gaccgacgat ctggacctcc cacaggacct tgcccgacgc agcatcgaac gcgagcacga
     1381 agccgtccga gtagcctcgg aacagcttgc catcgaggaa cgcgacgccg cggttgttgc
     1441 cgagcgtgac gccgggcgcg gccttgcgaa catgcctcca cttctcccga caggtcgctc
     1501 cgtccaccgc aaacgtggtg gcgttgtggg tgaaatagag gactccgttg atcacgatcg
     1561 gtccggtttc cccacccggg cccggctccc cggtgtcaaa cacgcacgtg cgcctgagcg
     1621 acggcgcgac ccggggcgac aggccctgga tcggcgagaa ccgctcgccc gcgaggttcg
     1681 cgttgtacgt ccgccagtcg accgatccgt gttgtgccag agatgcctcc ggcgcgatcg
     1741 gtaactgcga ttctttataa caacccacga ccgccgcgac gacgacacct gcccacgata
     1801 tcctgttacc taccctcatg gtccactccc ttcggcgagg gaccgtctgc aagccgctca
     1861 ccacgcgggt gggcggtggc agtctcccgt gtcggcgatc gggtcactcc cctggtcact
     1921 cccctggcca cgcccctggc cacgccgatc agcgcgcgat agtgcggtga cccggatgac
     1981 gggcgtatcg cgcccggatc cgccggcgcg ctgccgcggt ccgatcgtcg cgtcacccga
     2041 tcgcgcgccg ggagtcggcc ga
//
LOCUS       DASVOR010001289         5532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_13947,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001289 DASVOR010000000
VERSION     DASVOR010001289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5532
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13947"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /locus_tag="VF516_24610"
     CDS             complement(<1..847)
                     /locus_tag="VF516_24610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110947.1"
                     /translation="MPDPDARYYIPDERVGQYARLDELGRGGQSIVWRAFDVFVGREV
                     ALKELLEPAVLVESGSSHTAPRERFLREVRLTAQLDHPGIVAVHDLARRPDGTLVCAQ
                     KLVRGDTLKARLQGCATLAERLALLPHVTDACQAVAYAHSRGIVHRDLKPSNIMVGAF
                     GETVVLDWGLATQRGEAEPAVGDSLPGSGSELTASGAVLGTPAYMSPEQARGAVAEVD
                     ERSDVFSLGVILYEVLTGRRPFDGDGAAEVIAGVLRGDYPPVREVCPAAPAELAAVAE
                     RALRRD"
     gene            complement(1188..2444)
                     /locus_tag="VF516_24615"
     CDS             complement(1188..2444)
                     /locus_tag="VF516_24615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110948.1"
                     /translation="MLASGCAPASRATPSPDRRAQDAIRWRRVHHETFDAPFADPPWV
                     EDTYGDASPYHVDAFSEDGAFFTERGGATFAANLARFRSFRRSSRYGEGGWLTVELYG
                     RDSDRDGVPETGGQFVSGNGVARLISTRHYDGAIIRSTEPLPPRYRVEVTVGNIRFGG
                     ARDGQWTHDGKINGYDGDEVADPWFFRDGDPTPRPATTQNGLYFLCITDYPRPAPHNN
                     VFIHHHRKVVMDTDNNEYSVEPGATLRGPWSMVWSPETRRGEPDGSHYVSMLWLNGES
                     FGHDWFGNDFVSYTPGGWQTDAPIFTDKYLDGERYRFAIERDGERYTMSVTGRFHHGG
                     VTTYRASRRFRDAPVTWHYNQAPGEYVSADHDEVRRYGDAELHTWPAGSAYPDYFFFG
                     DPHINFYEGTAEFDDLDLYLPEDGTP"
     gene            2791..4458
                     /locus_tag="VF516_24620"
     CDS             2791..4458
                     /locus_tag="VF516_24620"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8110949.1"
                     /translation="MRTTGLAVLLLATSARASEPMEPDDLPAPRPEIARGEPKVAMPA
                     VPGFEPPVIEPGFHRPRELRLRGAPLRGQEMRVKGYVTSIYDCVAELALSNPDLRRAA
                     LVEAIDRDPRHCDQPRFYLGEDKGAPVEASITVVDVPRRPSKPERDALSLAELGKWPK
                     VPTLAVHDPVIVTGTWALRSPRGEYDSNGLLVYKALDHVAPGTASAVLPAAAPVTDPE
                     PEVDVAVVTKAPLRRLVANPVFNTSVDHLNACNQRILAGRYDEAIAECEAAAAAWPGN
                     HLAWYEAASAYIARRQWREARDAAARAVAMRPDVAMYQLYYGMALYEAEQQRVREEQA
                     AREHRQPDEVAIDPSALELTAARDALFRATRLGPELWRAHHYLGRVYRDLDDARHAAE
                     QFSQAIATHPSYLPPYVALTELYRRWGYADQALAVAMLGVGHVPPADAPALWYEAGMA
                     LHAKRAGDKAIEAFDKAVAGDHPDMFTRFLRGQVYLRKGRLADARQELEQVVRSTDPQ
                     VAEVRQLAVELLRQVAHKQRLKAGAGGGDCKRYGGCVEDTEGVIDRK"
     gene            complement(4510..>5532)
                     /locus_tag="VF516_24625"
     CDS             complement(4510..>5532)
                     /locus_tag="VF516_24625"
                     /inference="COORDINATES: protein motif:HMM:NF025418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule assembly Wzi family protein"
                     /protein_id="HEX8110950.1"
                     /translation="TERYPTALAVDRAYASAELGPIAAEVGRDVLVLGPAAHTLVGWS
                     TNPPPLDQLRLSGARPLALSRQLRLNAVYVLGRLAAPQTYPGDLVSIARAQLDIADRF
                     ELGGMQLLQLGGDGAPGFGLWDFVLEHVRRRDASASATDSSNRRLGFDVAARIAGFGG
                     ARITYQLMFEDLRKHFADALRYDADHVVGIETRWLTVEWQHTGVRSYEHTPRLTGFTS
                     GGRIVGDPLGPAAHAVFVGGRIPVRSGVVMPWAEVAALASDTYTFFEHGPIVRTGDGP
                     AELRFRLGARARLPVGGGLELDPEAALEDVERAAFVPGARRINAVLRAVLVWRPSAES
                     ATLRAR"
BASE COUNT          813 a         1931 c         2012 g          776 t
ORIGIN      
        1 gatcgcggcg gagcgcgcgc tcggccacgg cggccagctc cgccggagcc gcggggcaga
       61 cctcgcgcac cggcgggtag tcaccgcgca gcacgccggc gatgacctcc gccgcgccgt
      121 cgccgtcgaa cggccggcgc ccggtgagca cctcgtagag gatcacgccg aggctgaaca
      181 cgtcgctccg ctcgtcgacc tcggccaccg cgccgcgcgc ctgctccggg ctcatgtacg
      241 ccggcgtacc cagcacggcc ccggaggcgg tgagctcgga gccggatccc ggcagcgaat
      301 ccccgaccgc cggctcggcc tcgccgcgct gcgtggccag cccccagtcg agcacgacgg
      361 tctcgccgaa ggcgccgacc atgatgttcg agggcttgag gtcgcggtgg acgatgcccc
      421 gcgagtgcgc gtaggccacg gcctgacacg cgtcggtcac gtgcgggagc agcgcgagcc
      481 gctcggcgag cgtcgcgcag ccctggaggc gggccttcag cgtgtcaccg cggaccagct
      541 tctgggcgca gaccagcgtg ccgtcgggcc ggcgcgccag atcgtggacc gcgacgatgc
      601 cagggtgatc gagctgcgcc gtcaagcgca cctcgcgcag gaaccgctcg cgtggcgccg
      661 tgtgtgacga gcccgactcg acgagcaccg cgggttcgag cagctccttc agcgccacct
      721 cgcgtccgac gaacacatcg aacgcgcgcc acacgatcga ctggccgccg cgcccgagct
      781 cgtcgaggcg cgcgtactgg cccacccgtt cgtcggggat gtagtacctg gcatcaggat
      841 ccggcatgga gctacctccg cgttacagta cctccgccgg gctcgccctc cagtggctgc
      901 tgccggtata ctggttcggc gtgaacgtga cggtactggg tgaaggatac agagcctgaa
      961 acggcttgct ggggccgtcg tccgctggcg tcgagaacgc gcctgcaagg ttctcgagga
     1021 cctggcgctg ccactggtag aacacgctcg gctggagctg ttttccgatc gcacgcggat
     1081 tgcaatgtcc tcgtgcatag acgacggagg aactcccaat gtgacgttga tggaataccg
     1141 aaggggtcgc atcacggaaa gagtaccccc gagcgagcca gctggcgtca tggcgtcccg
     1201 tcctccggga ggtagagatc gaggtcgtcg aactcggccg tgccctcgta gaagttgatg
     1261 tgcgggtcgc cgaagaagaa gtagtccgga taggccgagc ccgccggcca ggtgtgaagc
     1321 tccgcgtcgc cgtagcggcg gacctcgtcg tggtcagcgg agacatactc gcccggggcc
     1381 tggttgtagt gccaggtcac cggggcgtcg cggaaccggc gtgacgcccg gtaggtcgtg
     1441 acgccgccat ggtgaaaccg gccggtgacc gacatcgtgt aacgctcgcc gtcgcgctcg
     1501 atggcgaagc ggtagcgctc tccgtcgagg tacttgtcgg tgaagatcgg cgcgtcggtc
     1561 tgccagccgc cgggcgtgta cgacacgaag tcgttgccga accagtcgtg gccgaagctc
     1621 tcgccgttga gccacagcat cgagacgtaa tgcgagccgt cgggctcgcc gcgccgggtc
     1681 tcgggactcc acaccatcga ccacgggccg cgcagcgtgg cgccgggctc cacgctgtac
     1741 tcgttgttgt cggtatccat cacgaccttg cggtggtggt ggatgaagac gttgttgtgc
     1801 ggcgccggcc gggggtagtc ggtgatgcac aggaagtaca gcccgttctg cgtggtggcc
     1861 ggccgcggcg tcggatcgcc gtcgcggaag aaccatggat cggcgacctc gtcgccgtcg
     1921 taaccattga tcttgccatc gtgggtccac tggccatcgc gcgcgccgcc gaagcggatg
     1981 ttgccgaccg tgacctcgac gcggtagcgc ggcggtagcg gctcggtcga gcggatgatc
     2041 gcgccatcgt agtggcgggt ggagatcagc cgcgcgacgc cgttgccgct cacgaactgg
     2101 ccgccggtct ccgggacgcc gtcgcgatcg gagtcgcggc cgtacaactc gacggtgagc
     2161 cagccgccct cgccgtagcg cgaggagcgt cggaacgagc ggaagcgcgc caggttggcg
     2221 gcgaaggtcg cgccgccgcg ctcggtgaag aacgccccgt cctcggagaa cgcgtcgacg
     2281 tgatacgggc tcgcgtcgcc gtaggtgtcc tcgacccacg gtgggtccgc gaacggtgcg
     2341 tcgaaggtct cgtgatggac ccgtcgccag cggatcgcgt cttgcgccct gcgatccggc
     2401 gacggcgtgg cccgcgacgc aggcgcacag ccgctcgcga gcatcgttgc gagcatcggg
     2461 gcccatgcgc ggcccctcat cgccatgttc tccgacctcc tgcgatcgtc accattcctg
     2521 gatcctagcg cagaggttct cggcgccacc catcgcgttc gggttgacgc cgcagcagcg
     2581 gggtcgagac cgctcatcgc tcggctgtcg cacccgcgga tggtcgtcga tatcgtcccg
     2641 gccgttgcca gtggttgtca ttgaccgacg ggtgcgcgag atacagcaag tgtcgtggcg
     2701 gggccaaggt gggtggcaga tttcgatcgc gcccccgagg ccgtcgtttc gcgcatcgat
     2761 cctggacggt ccgcggtagc ctggctaccc atgcggacca ccggactggc ggtgctcctg
     2821 ctcgcgacgt cggcgcgagc cagcgagccg atggaacccg acgacctccc cgcgccaagg
     2881 cccgagatcg cgcgcggcga accgaaggtg gcgatgcctg cggtgccggg cttcgagccg
     2941 ccggtgatcg agcccggctt ccaccggccg cgcgagctgc ggctgcgcgg cgcgccgctg
     3001 cgcgggcagg agatgcgggt caagggctac gtgacgtcga tctatgactg cgtcgcggag
     3061 ctcgcgctga gcaatcccga cctccgccgc gccgcgctgg tggaggccat cgaccgggat
     3121 ccccggcatt gcgaccagcc gaggttctac ctgggggagg acaagggcgc cccggtcgag
     3181 gcatcgatca ccgtggtcga cgtgccgcgt cggccgtcga agcccgagcg cgacgcgctg
     3241 agcctcgccg agctcggcaa atggccgaag gtgccgaccc tcgcggtcca tgatcctgtg
     3301 atcgtcaccg ggacctgggc gctccggtcg ccgcgcggcg agtacgacag caacgggctc
     3361 ttggtctaca aggcgctgga ccacgtggcg cccggcacgg cgagcgccgt gcttcccgcg
     3421 gccgcgccgg tgaccgaccc ggagccggag gtcgacgtcg ccgtcgtgac caaggcaccg
     3481 ctgcgccggc tcgtcgccaa tccggtgttc aacacctcgg tcgatcacct gaacgcctgc
     3541 aaccagcgga tcctcgccgg ccggtacgac gaggcgatcg ccgagtgcga ggcggccgcg
     3601 gcggcgtggc ccggcaatca cctcgcgtgg tacgaggccg cgagcgcgta catcgcgcgg
     3661 cgccagtggc gcgaggcgcg ggacgccgcc gcgcgcgcgg tggcgatgcg gcccgacgtg
     3721 gcgatgtatc agctgtacta cggcatggcg ctctacgagg ccgaacagca gcgcgtccgc
     3781 gaggagcagg cggcgcgcga gcaccgacaa cccgacgagg tcgcgatcga cccctcggcg
     3841 ctcgagctca ccgcggcgcg cgacgcgctg ttccgtgcca cgcggctcgg ccccgagctg
     3901 tggcgcgcac atcactacct cgggcgcgtc tatcgcgatc tcgacgacgc gcggcacgcg
     3961 gccgagcagt tcagccaggc gatcgcgacc cacccgagct atctgccgcc gtacgtcgcg
     4021 ctgaccgagc tctatcggcg gtggggttac gccgatcagg cgctcgcggt ggcgatgctc
     4081 ggcgtcggcc acgtgcctcc cgccgacgct cccgcgctgt ggtacgaggc cggcatggcg
     4141 ctgcacgcca agcgcgccgg tgacaaggcg atcgaggcgt tcgacaaggc ggtcgccggc
     4201 gatcacccgg acatgttcac gaggttcctg cgcggtcagg tgtacctccg caagggccgg
     4261 ctcgcggacg cccggcagga gctggagcag gtcgtgcgct cgaccgatcc gcaggtcgcc
     4321 gaggtccggc agctcgcggt cgagctgctg cggcaggtcg cgcacaagca gcggctcaag
     4381 gccggagccg gcgggggcga ctgcaagcgc tatggcggct gcgtggagga caccgagggc
     4441 gtgatcgacc ggaagtagcc tgcgcgctcg tcgaggcgat cggcacgatc cgcttgcccg
     4501 tcagctccat catcgggccc gcaaggttgc agattccgcc gacggccgcc acacgagcac
     4561 ggcgcggagc acggcgttga tccggcgcgc gccgggcacg aacgcggcgc gctcgacgtc
     4621 ctccagcgcg gcctccggat cgagctcgag gccgccgccg accggcagcc gggcacgcgc
     4681 gccgagccgg aaccgcagct cggccgggcc atcgccggtc cgcacgatcg gcccgtgctc
     4741 gaagaacgtg tacgtatcgc tcgcgagcgc ggcgacctcg gcccacggca tcaccacgcc
     4801 cgatcgcacc gggatccggc cgccgacgaa caccgcgtgt gccgccgggc cgagcggatc
     4861 gccgacgatc cggccgccgc tcgtgaaccc ggtgagccgc ggcgtgtgct cgtacgagcg
     4921 cacgccggtg tgctgccact ccacggtcag ccaccgggtc tcgatgccga cgacgtggtc
     4981 ggcgtcgtag cgcagcgcat cggcgaagtg cttgcgcagg tcctcgaaca tcagctggta
     5041 cgtgatccgc gcgccgccga acccggcgat gcgggcggcg acgtcgaacc cgagccggcg
     5101 attcgagctg tcggtcgcgc tcgccgaggc gtcgcgccgc cgcacgtgct cgagcacgaa
     5161 gtcccacagc ccgaacccgg gcgcgccgtc gccgccgagc tgcagcagct gcatcccacc
     5221 gagctcgaag cggtcggcga tgtcgagctg ggcccgcgca atgctgacca gatcgccggg
     5281 gtaggtctgc ggcgcggcca gccggccgag cacgtagacc gcgttcaggc gtagctgccg
     5341 cgacagcgcc agcggtcggg cgccagacag ccggagctgg tcgagcggcg gcgggttggt
     5401 gctccagccg acgagggtgt gcgccgcggg gccgagcacg agcacgtcgc ggccgacctc
     5461 ggcggcgatc ggtccgagct cggcggaggc gtaggcgcga tcgacggcga gcgcggtcgg
     5521 gtagcgctcg gt
//
LOCUS       DASVOR010001290         2403 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363839,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001290 DASVOR010000000
VERSION     DASVOR010001290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363839"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VF516_24630"
     CDS             complement(<1..859)
                     /locus_tag="VF516_24630"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8110951.1"
                     /translation="MTIQIGIDLGGSNLRAAAFGDEDGATPLASHREPVGDDRAPEAI
                     VERVARTIERLAGGAAGPVTVGVGIAALLRDRRGTVAQSPHLRWRDVPFGELLGRRLG
                     TRYRLGVYNDVNAITWGEAVAGAARGCRDVLAVYVGTGIGGGLIAGGQLVEGASNCAG
                     EIGHTKVRWDEAAAPCMCGGRGCVEAYVGGSYLVPRIRRELADPRRKTTVIERAGGSI
                     DAVMPSHVDDAALDRDAWAVGLWTELATLLAVALGNALAVVNSERLILGGGVLGRTPA
                     LRAMTEVGLA"
     gene            1150..>2403
                     /locus_tag="VF516_24635"
     CDS             1150..>2403
                     /locus_tag="VF516_24635"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8110952.1"
                     /translation="MGDDEEFVGRMLVAEGIVAADEVHELARAALQDGQGLLEQLRAD
                     GRISGDTLVSLRARMHGAAPAGPAMDLTPLVLPRPRPRGESAAVETRPAAAGDDHGGA
                     FDGDGEIDGDGEFDRFPVPDWDHYEPIRLLGRGGMGRVFLARDLRLGREVAIKFMLGE
                     TPQLARRIVAEARAQARVNHDRVCKVYEVGEIRGRVYIAMQYINGRPLSELGSELTLE
                     EKVIVIREAAFGMAEAHRVGLIHRDLKPSNIMVERAPDGELRPYVMDFGVARDWMGSV
                     TLTGTVVGTPNYMAPEQAQGEGTQLDRRADVYSLGATLYALLTRRPPAEGSNALAVLM
                     RVCTDEPVRPRTIDPDVPVDLEAIAMKCLEKERAARYDSARALADDLTRFLAGEPVAA
                     RASAGVRYRVRKWLRRRRRLVAGVAI"
BASE COUNT          330 a          857 c          897 g          319 t
ORIGIN      
        1 ccgccagacc gacctcggtc atcgcgcgca gcgcgggggt gcgaccgagc acgccgccgc
       61 cgaggatcag ccgctcggag ttcacgaccg cgagcgcgtt gccgagcgcc accgcgagca
      121 gcgtcgccag ctcggtccac agcccgaccg cccacgcgtc gcggtcgagc gcggcgtcgt
      181 cgacgtggct cggcatgacc gcgtcgatcg agccgccggc gcgctcgatc accgtggtct
      241 tccgccgcgg atccgcgagc tcgcggcgga tccggggtac caggtagctg ccgccgacgt
      301 aggcctcgac gcagccgcgc ccgccgcaca tgcacggcgc ggcggcctcg tcccaccgga
      361 ccttggtgtg gccgatctcg ccggcgcagt tgctcgcgcc ctcgaccagc tggccgccgg
      421 cgatcaaccc accgccgatc ccggtgccga cgtagaccgc gagcacgtcg cggcagccgc
      481 gggccgcgcc cgccaccgct tcgccccagg tgatcgcgtt gacgtcgttg tagacgccga
      541 gccggtagcg cgtgcccagc cggcggccga gcagctcgcc gaacggcacg tcgcgccagc
      601 gcaggtgggg cgactgcgcg accgtgcccc gtcgatcgcg gagcagcgcg gcgatcccga
      661 cgccgaccgt gaccgggccg gccgcaccgc cggccagccg ctcgatcgtc cgtgccacac
      721 gctcgacgat cgcctccggc gcgcgatcgt cccctacggg ctcccggtgg ctcgcgagcg
      781 gagtcgcgcc atcctcgtcg ccgaacgccg ccgcgcgcag gttcgagccg ccgaggtcga
      841 tcccgatctg gatcgtcaca ccgcgagtcg taccgcgggc gccgaccggg ccgaaagcgc
      901 cggccggcgg aacgagcgct tccagcgcac cgtgattgcc aatcgcgtgc gcatcgctcc
      961 ggccgctgcg cagcgcacac ggtgaccacg ccgggcgaat cgccggacta cgcgtttcgg
     1021 tcgtcagtgg ggctgcttac gcttcatggc ggcctcacgc tcggctaccg gaccactccc
     1081 gcagaagtca ggcgccattc ccgagaatgc tattcatttg tcctgccgcg gtccgggtat
     1141 tcttccagca tgggggacga cgaggagttt gtgggccgga tgctggtcgc cgaggggatc
     1201 gtcgcagcgg acgaggtgca cgagctcgca cgcgcggcgt tgcaggacgg gcagggtctg
     1261 ctggagcagc tgcgcgcgga cggtcggatc tcgggagata cgctggtctc gctccgcgcg
     1321 cgcatgcacg gagcagcccc ggccggcccg gcgatggacc tcactccgct cgtcctcccg
     1381 aggccgcggc cacggggcga atcggcggcg gtcgagaccc gacccgcggc cgcgggcgat
     1441 gaccatggcg gtgcgttcga cggtgacggc gagatcgacg gtgacggcga gttcgaccgg
     1501 tttccggttc cggactggga tcactacgag ccgatccggc tgctcggccg gggcggcatg
     1561 ggacgggtgt tcctggcccg cgatctccgg ctcgggcgcg aggtcgcgat caagttcatg
     1621 ctcggggaga ccccccagct cgcgcggcgg atcgtggcgg aggcgcgggc ccaggcgcgg
     1681 gtcaaccacg accgggtgtg caaggtctac gaggtcggcg agatccgcgg ccgggtctac
     1741 atcgcgatgc agtacatcaa cgggcgaccg ctgtcggagc tcggcagcga gctgacgctc
     1801 gaggagaagg tgatcgtcat ccgcgaagcg gcgttcggga tggcggaggc gcaccgggtc
     1861 gggctgatcc accgcgatct caagccatcg aacatcatgg tggagcgcgc gcccgatggc
     1921 gagctgcggc cgtacgtcat ggacttcggg gtggcgcgcg actggatggg cagcgtgacg
     1981 ctgaccggca cggtggtggg cacgccgaac tacatggcgc ccgagcaggc gcagggcgag
     2041 ggcacccagc tcgatcgccg cgcggacgtg tacagcctgg gcgccaccct gtacgcgctg
     2101 ctgacccggc ggccgccggc cgaaggctcg aacgcgctgg ccgtgctgat gcgggtgtgc
     2161 accgacgagc cggtgcggcc gcggacgatc gatcccgatg tcccggtgga cctcgaggcg
     2221 atcgcgatga agtgcctcga gaaggagcga gcggcgcgct acgactcggc gcgcgcgctc
     2281 gccgatgacc tcacgcggtt cctggcgggc gagccggtcg cggcgcgcgc ctcggccggg
     2341 gtacgctacc gggtgcgcaa gtggctgcgc cgacggcggc ggctggtcgc cggggtcgcg
     2401 atc
//
LOCUS       DASVOR010001291         5704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_480921,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001291 DASVOR010000000
VERSION     DASVOR010001291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5704
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480921"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VF516_24640"
     CDS             <1..426
                     /locus_tag="VF516_24640"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8110953.1"
                     /translation="AAIDYKAVDVRDALRQHCPQGIDVFFDNVGGDILDAALTRLAMR
                     ARIVICGAISQYNNTTPVKGPSNYLSLLVNRASMTGMVVFDYAPRFPAAIAEMAQWVA
                     AGKLRSKEDIVRGLDQFPHALLKLFTGENHGKLMLELPA"
     gene            complement(443..3802)
                     /locus_tag="VF516_24645"
     CDS             complement(443..3802)
                     /locus_tag="VF516_24645"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8110954.1"
                     /translation="MKVGSASPGDLDRAEAATAPEARGGPTGTPPSPNVETRTSEPSE
                     PRAQPGAPPAVPRVTGGRYEILREHGRGGIGCVYRAHDRELGRDVAIKKLISRGNLVE
                     MRFFREAAITARLEHPGIVPVHEAGWWSDGTPFYAMKLIAGRPLRDLIAERFTVEQRL
                     GLLHHVVAVADAIAYAHARKVIHRDLKPSNVIVGEFGETVVIDWGLAKDLATNESAAD
                     GGDPLPAHRDGDLTSAGSVLGTPAYMAPEQRRGEAVDQRADVFAIGMMLWQLCSLEAA
                     PPADPLQRRAVLRRARVDDDLIAIIDKAIAGDPRHRYGDAGALVADLRAFTSGARIAA
                     RRYSPLAVVTHWIRRHRVLAISAATCLALLAVLVRTLLHAWVTERVADLGVTQAEVEA
                     GRQALLHEDTAQAQSHLARAYLRGERSPGVAFMLSRALQPRLAEQARLPSAAGRMWPA
                     EFSPDGRQIVTPDETSAQVWDTETHRRLFLLPHGGEVYRAVYTADGARIVTAARGAVR
                     IWDAASGALVRALTPPPGHDTLTYFRLDRSRDGKLVAAIDVNGAIVDVWDASSGTLVA
                     ELPNDGWEYPLIAFTGDGRWLATGGGSDVRVFDTRTWARVMTVAGPRIRSMAIDPTAP
                     RLITGSFDGDAAIWAIPSGERVYHLRELGDPVDAVAFAPGGATVATATRDGMIQVWDA
                     SSGKLRSTNHQLRTKILSLEFDRTSALVVAGGSSGKVLVEDAASGMPLSVLEGPSNVV
                     WGRFDPSARRVVGASWDGTARIWDAASPYLRWRSPTVNDVCRIVASLEPDRRFVAVGC
                     LGHATHIWDTAHDRLLAELPGTTQVGGGFTSALPAVSAAGDRAAVARDRTVVIYELPG
                     GRPLRTIRHGAAVNAVSFATTGHDLISGAIDGSVLVTRDDGEPVALPGFAAGVDAAGF
                     LSDGRPVAAAGNQLRVYDAERRTVLAELGLPARVHTLRSSSDGRRLVTGPRSIDAPTP
                     AVLWDVEHYQRIGPLAGHVGIVWSARFVRDGREILTSGGDGTVRSWDATSGRLLHTYR
                     GSTRFLTDATLDPEGAMIVAVGGDGLIWFWDAANERPLWTLLGHQPYVIALHFEAGDL
                     VTRGFAGDVARWTFPEPDLDALPELLRELSK"
     gene            complement(3862..5403)
                     /locus_tag="VF516_24650"
     CDS             complement(3862..5403)
                     /locus_tag="VF516_24650"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX8110955.1"
                     /translation="MKRRDLFKNSLAASALAGLSAVAPRTRRAHAAPPPAPAPALASA
                     SSPEFPRAPGLTRSIAEFIVGTKYEDIPADVLELGKKSILDGFGLALAGSVSAMGPLV
                     QKYVQSFGASPTRASVIGTRLKCHPRFAALANGVSIHADDFDDTQLAAAKDRIYGLLT
                     HPTVPVLPPAFALCEVGRRSGKDLMLAYHLGVEVECKIAEAIAPRHYDEGFHTTGTIG
                     SFGSAAACAKLRGLDAKQTAYALGIAATEGGGFRHNFGSMTKPFHAGHAAENGTVAAD
                     LAALGWTASDDILEAPLGFFQAAGGSFDPAAIVGRAGKPWTFASPGVSIKPHPSGSLS
                     HPAMGEMQRLIREHKLKPADIEKIDLGANHAMTTSLLHHHPTTGLQGKFSMEFSLSIL
                     LVEGKAGLAQYQDAVVQRPDVQDMIKRVNFHVDPEAERAGLDKMTSILKITLKDGKVL
                     SGRAEFAKGSPANPMSYDEVADKFRGCAEFTKWPAAKAEAVIAAVKSLENLSDVGKLT
                     AALTA"
     gene            complement(5450..>5704)
                     /locus_tag="VF516_24655"
     CDS             complement(5450..>5704)
                     /locus_tag="VF516_24655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110956.1"
                     /translation="KAHGLNYPMADGKLEPDWLALFTDFSDRFVIGSDQHYPETADPV
                     QRWQEVVRLFNQLPEPVRKKIGTENVARIYGKAVAPSLKH"
BASE COUNT          832 a         1995 c         2067 g          810 t
ORIGIN      
        1 gccgcgatcg actacaaggc ggtcgacgtc cgggacgcgc tgcgccagca ctgcccgcag
       61 ggcatcgacg tgttcttcga caacgtcggc ggcgacatcc tcgacgcggc gctcacccgg
      121 ctcgcgatgc gcgcccgcat cgtcatctgc ggcgcgatct cgcagtacaa caacacgacg
      181 ccggtcaagg gcccgagcaa ttacctgtcg ctgctggtca accgcgcgtc gatgaccggc
      241 atggtggtgt tcgactacgc gccgcggttc ccggccgcga tcgccgagat ggcgcagtgg
      301 gtggcggccg gcaagctgcg ctcgaaggag gacatcgtgc gcgggctcga ccagttcccg
      361 cacgcgctgc tcaagctgtt caccggcgag aaccacggca agctgatgct ggagctgccg
      421 gcctgaccga ccgcgcacac cgtcacttcg agagctcgcg cagcagctcg ggcagcgcgt
      481 ccagatcggg ctccggaaac gtccagcgcg cgacgtcgcc ggcgaacccg cgcgtcacca
      541 ggtcgccggc ttcgaaatgc agggcgatga catacggctg gtgtccgagc agcgtccaca
      601 gcggccgctc gttcgcggcg tcccagaacc agatcaggcc gtcgccaccg acggccacga
      661 tcatcgcgcc ctcggggtcg agggtcgcat cggtcaggaa ccgcgtgctg ccgcgatagg
      721 tgtggagcag ccgcccgctc gtcgcgtccc acgatcgcac ggtgccgtcg ccaccgctgg
      781 tgaggatctc gcggccgtcc cgcacgaatc gcgccgacca cacgatgccg acgtggccgg
      841 cgagcggccc gatgcgctgg tagtgctcga cgtcccagag cacggcgggc gtcggcgcgt
      901 cgatcgagcg cggaccggtg accaggcgac ggccgtccga tgagctgcgc agcgtgtgca
      961 cgcgggcggg caggccgagc tcggcgagca ccgtgcggcg ctcggcgtcg tagacccgga
     1021 gctggttgcc cgcggccgcc accggacggc catcgctgag gaacccggcc gcatcgacgc
     1081 cggccgcgaa ccccggcaac gcgaccggct cgccgtcgtc ccgcgtgacg agcaccgagc
     1141 cgtcgatcgc gccgctgatg aggtcgtgac cggtcgtcgc gaacgagacc gcattgaccg
     1201 cggcgccatg gcggatcgtg cgcagcggcc ggccgcccgg cagctcgtag atcacgacgg
     1261 tgcggtcgcg agcgacggca gcgcgatcgc cggcagccga gaccgcgggc agcgccgacg
     1321 tgaagccacc gccgacctgc gtcgtgccgg gcagctcggc gagcagccga tcgtgcgcgg
     1381 tgtcccagat gtgggtcgcg tggcccaggc agccgaccgc gacgaaccgg cgatcgggct
     1441 cgaggctcgc gacgatcctg cagacgtcat tgaccgtcgg cgatcgccag cgcagatagg
     1501 gcgacgccgc atcccagatc cgcgccgtgc catcccacga agcaccgacg acgcggcgcg
     1561 cgctcggatc gaagcgcccc cacacgacgt tgctgggtcc ctcgagcacc gagagcggca
     1621 tcccgctggc cgcgtcctcg accaggacct tgccgctgga gcccccggcg acgacgagcg
     1681 ccgacgtccg gtcgaactcg agcgacagga tcttggtgcg aagctggtgg ttggtgctcc
     1741 gcagcttccc ggatgacgcg tcccacacct ggatcatgcc gtcccgggtc gcggtcgcga
     1801 ccgttgcccc gccgggggcg aacgccacgg catcgaccgg atcgccgagc tcgcggaggt
     1861 ggtagacccg ctcgccgctc gggatcgccc agatcgccgc gtccccatcg aagctccccg
     1921 tgatcaggcg cggagcggtc ggatcgatgg ccatgctgcg aatccgcggg cccgccacgg
     1981 tcatgaccct cgcccacgtg cgggtatcga acacccgcac gtcgctgccg ccgccggtcg
     2041 cgagccatcg gccgtcaccg gtgaacgcga tcaaaggata ctcccagccg tcattgggca
     2101 gctcggcgac cagggtgccg ctggacgcat cccagacatc cacgatcgcg ccattcacat
     2161 cgatcgcagc gacgagcttg ccgtctcgag atcggtccag gcggaagtag gtcagcgtgt
     2221 cgtgcccggg cggcggggtc aacgcgcgca cgagcgctcc gctggcggcg tcccagatgc
     2281 gcacggcgcc ccgtgcggcg gtgacgatcc gggcaccgtc ggccgtgtac accgcgcgat
     2341 acacctcgcc gccgtggggc aaaaggaaca gcctgcgatg ggtctcggtg tcccatacct
     2401 gggcggacgt ctcgtcggga gtgacgatct ggcggccatc gggggagaac tctgcgggcc
     2461 acatacgacc cgccgcgctc gggaggcgcg cctgctccgc gagccgcggc tgcagcgcgc
     2521 gagacagcat gaacgcgacg cccggggagc gctcgccgcg cagataggcg cgtgcgaggt
     2581 ggctctgcgc ttgcgcggta tcctcgtgca ggagcgcctg gcggccggcc tcgacctcgg
     2641 cctgcgtgac gccgaggtcc gcgacgcgct cggtcaccca cgcgtgcagc agcgtgcgca
     2701 ccagcaccgc gagcagcgcc agacaggtcg ccgccgagat cgccagcacg cggtgcctgc
     2761 ggatccagtg ggtcaccacc gcgagcggcg agtagcggcg cgccgcgatg cgggcgccgg
     2821 aggtgaacgc ccgcagatcc gccaccagcg cgccggcatc gccgtagcgg tgccgcggat
     2881 cgccggcgat cgccttgtcg atgatcgcga tcaggtcgtc gtcgacccgg gcgcggcgca
     2941 gcacggcacg gcgctgcagc gggtcggccg gcggcgcggc ctcgagcgag cacagctgcc
     3001 acagcatcat cccgatcgcg aacacatcgg cgcgctgatc gaccgcctcg ccgcgccgct
     3061 gctcgggagc catgtacgcc ggcgtgccga gcacgctccc cgccgacgtc agatcgccgt
     3121 cgcgatgcgc ggggagcggg tcgccgccat cggcggcgct ctcgttcgtc gcgagatcct
     3181 tggccaggcc ccagtcgatc accacggtct cgccgaactc cccgacgatg acgttcgacg
     3241 gcttgaggtc gcggtggatg accttgcggg catgggcgta cgcgatcgcg tcggcgaccg
     3301 cgacgacgtg gtgaagcaga ccgagccgct gctcgaccgt gaatcgctcc gcgatcagat
     3361 cgcgcagcgg tcgacccgcg atcagcttca tcgcatagaa cggcgtgccg tcggaccacc
     3421 agccggcctc gtggaccggc acgatcccgg ggtgctcgag gcgcgcggtg atcgcggcct
     3481 cgcggaagaa ccgcatctcg acgaggttgc cgcgcgagat cagcttcttg atcgccacgt
     3541 cgcgacccag ctcgcggtcg tgggcccggt acacacagcc gataccgccg cgaccgtgct
     3601 cgcgcaggat ctcgtagcgg ccgcccgtga cccgcgggac cgcaggcggg gcgcccggct
     3661 gcgctcgagg ctccgacggt tcgctggtcc gggtctcgac gttgggcgac ggcggcgtac
     3721 ccgttggacc gccgcgcgcc tcgggcgccg tggctgcctc cgcgcgatcc agatcgcccg
     3781 gactggccga tcccaccttc atacgaccct acaccttagc aatcccggtc agacgtacaa
     3841 gacccgtcgc ggcgccagcc gtcaggcggt cagcgcggcg gtgagcttgc cgacgtcgga
     3901 caggttctcg agcgacttga ccgcggcgat caccgcctcg gccttggcgg cgggccactt
     3961 cgtgaactcg gcgcagccgc ggaacttgtc ggcgacctcg tcgtagctca tcgggttggc
     4021 cgggctgccc ttggcgaact cggcgcggcc ggacaggacc ttgccgtcct tgagggtgat
     4081 cttgaggatg ctggtcatct tgtcgaggcc ggcccgctcg gcctcggggt cgacgtggaa
     4141 gttgacccgc ttgatcatgt cctggacgtc ggggcgctgc acgacggcgt cctggtactg
     4201 cgccaggccg gccttgccct cgaccagcag gatgctgagc gagaactcca tgctgaactt
     4261 gccctgcagg ccggtggtcg ggtggtgatg aagcagcgag gtggtcatgg cgtggttggc
     4321 gccgaggtcg atcttctcga tgtcggcggg cttcagcttg tgctcgcgga tcaggcgctg
     4381 catctcgccc atcgccgggt gggacagcga gcccgacggg tggggcttga tcgacacgcc
     4441 ggggctcgcg aacgtccacg gcttgccggc ccggccgacg atcgcggcgg gatcgaagct
     4501 gccgcccgcg gcctggaaga agccgagcgg cgcctcgagg atgtcgtcgg aggcggtcca
     4561 gcccagggcc gcgaggtcgg cggcgacggt gccgttctcc gccgcgtggc cggcgtggaa
     4621 cggcttggtc atcgagccga agttgtggcg gaacccgccg ccctcggtgg cggcgatgcc
     4681 gagcgcgtag gcggtctgct tggcatcgag gccgcgcagc ttcgcgcacg ccgcggcgct
     4741 gccgaacgag ccgatggtgc cggtggtgtg aaagccctcg tcgtagtggc gcggcgcgat
     4801 cgcctcggcg atcttgcact cgacctcgac gccgaggtga taggcgagca tgaggtcctt
     4861 gccggagcgg cggccgacct cgcacagcgc gaacgcgggc ggcagcaccg ggacggtcgg
     4921 gtgggtcaag aggccgtaga tccggtcctt ggccgcggcg agctgggtat cgtcgaagtc
     4981 gtcggcgtgg atcgacacgc cgttggcgag cgcggcgaac cgcgggtggc acttgagccg
     5041 ggtgccgatg acgctggcgc gggtcggcga ggcgccgaac gactgtacgt acttctggac
     5101 cagcggcccc atcgccgaca ccgagccggc gagcgcgagg ccgaagccgt cgaggatcga
     5161 cttcttgccg agctcgagga cgtcggccgg gatgtcctcg tacttcgtgc cgacgatgaa
     5221 ctcggcgatc gatcgggtca gccccggggc cctggggaac tcgggggacg aggccgaggc
     5281 caaggcggga gccggagccg ggggcggtgc ggcgtgggcg cgtcgcgtcc gaggagcgac
     5341 tgccgacaga cccgccagcg ccgacgcggc cagggagttc ttgaacaggt cacgccgctt
     5401 catggagctc tccttgtcgg tgcttcgtca gtgcttcgtc agtgcttcgt cagtgcttca
     5461 gggacggcgc cacggccttg ccgtagatcc gcgcgacgtt ctcggtgccg atcttcttgc
     5521 gcacgggctc ggggagctgg ttgaacagcc gcaccacctc ctgccagcgc tgcaccgggt
     5581 cggcggtctc cgggtagtgc tggtcgctgc cgatcacgaa gcgatcggag aagtcggtga
     5641 acagcgcgag ccagtcgggc tcgagcttgc cgtcggccat cgggtagttc aggccgtggg
     5701 cctt
//
LOCUS       DASVOR010001292         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363867,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001292 DASVOR010000000
VERSION     DASVOR010001292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363867"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VF516_24660"
     CDS             complement(<1..338)
                     /locus_tag="VF516_24660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8110957.1"
                     /translation="MAGSAATDARAAAGPVRLAAHGLRKSFGSVVVTDDVSFVARGGE
                     ALGIIGPNGAGKTTLFHLLAGSLPAGLLSYAEQRALEIGITLAGGAEIILLDEPTAGM
                     SHSETEAAVAL"
     gene            complement(338..1612)
                     /locus_tag="VF516_24665"
     CDS             complement(338..1612)
                     /locus_tag="VF516_24665"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8110958.1"
                     /translation="MRRKHSGIDRRTLLGWSMGGALGLALPGCKKPSDTGTGSSTSTG
                     GGDTREVVIGYVSPQTGPLAAFAEADAFIIERVTKALDAKGIMVDGKKARFTVKRVDS
                     QSNPTKAGEAAQGLISGDKVDLMVVGHTPDTTNPVGAVCEATKTPCISSLAPIGPWLA
                     GAPYEWTFHFFWDLPDIINVFTGMWDTLPTNKVVGALWPNDPDGSAWAPAFTKALTDK
                     GYKVIDPGRYPNLTTDFTVFIQKWKEAGVEILTGVPIPPDWAACWRQCAQQGFKPKIA
                     SIGKAILFPAAVEALGGGLAEGLSSEVWWSPAHPFKSSLTGESAADLAGAYPKQWSQP
                     LGFVYALFEVAGNALLRAQSTDKQKVRQAIAATKLDSMVGVIQFDDKHVGRTPLVGGQ
                     WTKGQKYPWDLHVVYNQTAPTIGKTAELKAIT"
     gene            1717..>2720
                     /locus_tag="VF516_24670"
     CDS             1717..>2720
                     /locus_tag="VF516_24670"
                     /inference="COORDINATES: protein motif:HMM:NF014795.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8110959.1"
                     /translation="MPTASAAFLDALTSAGVSYLFANFGSDHVALLEALAEAAATGRP
                     VPELVVCPAEMVALSAAQGVAQVTGRAQAVLVHVDCGTQALGGAVHNAARARVPVLIF
                     AGASPYTQHGELAGSRNEHIHWLQDVRDQRGIVRGYVKYDNEIRTGRTLDQIVFRALQ
                     IAQSDPPGPVYLVAPREVLEETVPPLRAAVTDYPALAPQALAPDDVRLLADALGRAHR
                     PLVITSYLGRNREAPAALARVCRRLGIGVLESVPSHVNFPADDPLYQGSQWNAQRPNP
                     VLAAADVILVLDSDVPWMPLVNPPRPDAAIYHLDVDPLKPHAPLWHIAARRTLCADAA
                     T"
BASE COUNT          366 a         1017 c          916 g          421 t
ORIGIN      
        1 agcgcgacgg ctgcctcggt ctcgctgtgg ctcatgccgg cggtcggctc gtcgagcagg
       61 atgatctcgg ccccgccggc gagggtgatg ccgatctcca gcgcccgctg ctcggcatag
      121 gacaaaaggc cggccggcag gctgccggcg agcaggtgga acagcgtggt cttgccggcg
      181 ccgttggggc cgatgatgcc gagcgcctcg ccgccgcgcg ccacgaatga cacgtcgtcg
      241 gtgacgacca cgctgccgaa cgacttgcgc aggccgtggg cggcgagccg gaccggcccc
      301 gccgccgcgc gcgcgtcggt cgcagcgctg ccggccatca ggtgatggcc ttgagctccg
      361 cggtcttgcc gatcgtcggc gcggtctggt tgtagaccac gtgcagatcc cacggatact
      421 tctggccctt ggtccactgc ccgccgacca gcggcgtgcg gccgacgtgc ttgtcgtcga
      481 actggatcac cccgaccatg gagtcgagct tggtggctgc gatcgcctgc cgcaccttct
      541 gcttgtcggt gctctgggcg cgcagcagcg cgttgccggc gacctcgaac agcgcgtaga
      601 cgaagccgag cggctgcgac cactgcttgg gatacgcgcc cgcgaggtcc gcggcggact
      661 ctccggtcag cgacgacttg aacgggtgcg ccgggctcca ccagacctcc gacgacagcc
      721 cctcggcgag cccgccgccg agcgcctcga ccgcggccgg gaacaggatc gccttgccga
      781 tgctggcgat cttgggcttg aaaccctgct gcgcgcactg ccgccagcac gccgcccagt
      841 cgggcgggat cggcacgccg gtgaggatct cgacgccggc ttccttccac ttctggatga
      901 acacggtgaa gtcggtcgtc aggttggggt agcgccccgg atcgatcacc ttgtagccct
      961 tgtcggtgag cgccttggtg aacgccggcg cccacgccga gccgtccgga tcgttgggcc
     1021 acagcgcgcc gacgaccttg ttggtcggca gcgtgtccca catcccggtg aacacgttga
     1081 tgatgtccgg caggtcccag aagaagtgga acgtccactc gtacggcgcg ccggccagcc
     1141 acgggccgat cggcgccagg ctggagatgc acggcgtctt ggtcgcctcg cacaccgcgc
     1201 cgaccgggtt ggtcgtgtcg ggcgtgtggc cgacgaccat caggtcgacc ttgtcgcccg
     1261 agatcaagcc ctgcgcggcc tcgccggcct tggtcgggtt ggactggctg tcgacccgct
     1321 tgaccgtgaa ccgcgccttc ttgccgtcga ccatgatccc cttggcgtcg agcgccttgg
     1381 tgacccgctc gatgatgaag gcatcggcct cggcgaacgc ggccagcggg ccggtctgcg
     1441 ggctgacgta gccgatcacc acctcgcggg tgtcgccacc gcccgtgctg gtgctgctgc
     1501 cggtgccggt gtccgagggc ttcttgcagc ccgggagcgc gaggcccagg gcaccgccca
     1561 tcgaccagcc gagcaaggta cgtcgatcga taccgctgtg tttgcgacgc atgatgatcc
     1621 cggttactgc caggcgctcc cgtgtgtcaa ccaggtttcg ctcgccgcgc tggtcaacag
     1681 tgttccgggc atgggaagta gagtgacgag agctccatgc ccaccgccag cgcagccttc
     1741 ctcgacgcgt tgacctccgc cggggtctcc tacctgttcg ccaacttcgg cagcgaccac
     1801 gtggcgctcc tcgaggcgct cgccgaggcc gccgcgaccg gccgccccgt ccccgagctc
     1861 gtcgtctgcc cggccgagat ggtcgcgctc agcgccgcgc agggcgtcgc ccaggtcacc
     1921 gggcgcgccc aggccgtcct ggtccacgtc gactgcggca cccaggcgct cggcggcgcg
     1981 gtccacaacg ctgcccgcgc gcgggtcccg gtgctgatct tcgccggggc gtcgccgtac
     2041 acccagcacg gcgagctcgc cggcagccgc aacgagcaca tccactggct gcaggacgtc
     2101 cgcgatcagc gcggcatcgt ccgcggctac gtcaagtacg acaacgagat ccgcaccggg
     2161 cgcaccctcg atcagatcgt cttccgcgcg ctccagatcg cccagagcga tccgcccggc
     2221 ccggtctacc tggtcgcgcc gcgcgaggtc ctcgaggaga ccgtgccgcc gctgcgcgcc
     2281 gccgtcaccg actacccggc gctcgccccg caggcgctcg cccccgacga tgtccgcctg
     2341 ctcgccgacg cgctcggccg tgcccatcgc ccgctcgtca tcacctcgta cctcgggcgc
     2401 aaccgcgagg cacccgcagc gctggcgcgg gtgtgccggc ggctcggcat cggcgtcctc
     2461 gagtcggtgc ccagccacgt caacttcccg gccgacgatc ccctgtacca gggtagccag
     2521 tggaacgcgc agcgccccaa cccggtgctc gcggcagccg acgtcatcct ggtcctcgac
     2581 agcgatgtcc cgtggatgcc gctggtcaac ccgccgcggc ccgacgccgc gatctatcac
     2641 ctcgacgtcg acccgctgaa gcctcacgcg ccgctgtggc acatcgccgc gcggcggacg
     2701 ttgtgcgccg acgccgcgac
//
LOCUS       DASVOR010001293         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_247236,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001293 DASVOR010000000
VERSION     DASVOR010001293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247236"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF516_24675"
     CDS             <1..442
                     /locus_tag="VF516_24675"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8110960.1"
                     /translation="QIAQNVRSELTAALGDRPCHIVGSDQRVHVEATGEYFYPDVLVT
                     CLEPRLVDPRPRSLVNPQLIVEVLSETTERYDRGDKWLAYRTIPSLTDYVMASSTRRE
                     VEHYQRSPDGSWTLRPPQRDGECVLASGVVLHVNRLYRLVPGLS"
     gene            1722..>2034
                     /locus_tag="VF516_24680"
     CDS             1722..>2034
                     /locus_tag="VF516_24680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110961.1"
                     /translation="MREKRLASLALSSFEGSPPLHETRMAGSELPARCCCSDWIAGLA
                     RDCAGRLAAIARREGLTASDALDVVQDAFHTLLHRPDTAALRDRPDDAARLLAAIVRN
                     AA"
BASE COUNT          297 a          726 c          687 g          324 t
ORIGIN      
        1 ccagatcgcc cagaacgtgc gcagcgagct gaccgccgcg ctcggcgatc gaccgtgcca
       61 tatcgtcggc agtgaccagc gggtccatgt agaggcgacc ggtgagtatt tctatcccga
      121 cgtcctggtc acgtgcctcg agccacgcct cgtcgatccg aggccgcggt cgctggtgaa
      181 cccgcagctg atcgtcgagg tgctctccga gacgaccgag cgatacgatc gcggcgacaa
      241 gtggctggcc taccgcacga tcccatcgct caccgactat gtgatggcct cgagcacccg
      301 ccgcgaggtc gagcactatc agcgctcgcc ggatggctcg tggacgctgc gtccaccgca
      361 gcgcgacggc gagtgcgtgc tcgcgagcgg cgtcgtcctc cacgtgaacc ggctgtaccg
      421 gctcgtgcct gggctcagct gatcgagatc aggagcagag accgccgcat cgacgcgcga
      481 tcggtggacc catcgcgcac ccgcgacgtg aacagaccgc cgggggcgct ccgtggaatc
      541 ggcgttgcgg aggtcacagt cgggcgaatc gccgcgcggc gaggacgcgg tgagagcgcg
      601 ccgtgcgtcg cgtcctcggg caggcacgtg gctcgcaacc gtgctgtctc gagcgtcggt
      661 caccgggtac cccgcgccgt ccggcgcgcg gcgacgagca caccgtgcca tggcgtcgcg
      721 atccgcgcgg cggacgagag tgcttcgccg gtgacgcgtc ggacctccgg cgccgttccg
      781 ccgctgtttc ctcacgttcg ctctcacgat ccgcgctgca gtcgagagcg cttcgccggc
      841 gacgcctcgg ctcggcgctc ctcggcaact gcatcctcac ggtgcccgcg gcggccgccc
      901 gcgcagcgat tgcgcctcgc gggtgcgctg gcttcaccgc agcgagcccg gagcgcgctc
      961 ctctcgcgcg agaccgccga tccgcgcgct gcgctcgctg gacaccgccg cgacgaactg
     1021 cggagcgcaa gccgatgccg cgcgctcctc cgctgccatc cggcatcgcc ggcctcgcgc
     1081 agctgcgtgc gcgggcaccc gacgaggtct tcgtcgcgat ctcgacgcca ccgcaaacca
     1141 gcccgctcgc cctgcgccgc gcggcaggac gtgctgccgc gatcatcgtg aggccgacat
     1201 cgcgacgcgg cggcatctca ccgacgaaga tcgacgattg ccgaccttct ccgccaccgg
     1261 tgaggagcat cgaaccaggc agctgctacg aagctcatgt ggtgacgagc ccgcgcaacg
     1321 caagaaccgg agccgggcga agcgtgcccg gtcgtacgcg cgccgtggca cgcccgccca
     1381 gtgctagcgc tgatcggttt cgtttttcaa cgacgagcct tcgtcgctca tgcgagacgg
     1441 tcctccgggg acgtccgggg tgtcgttgtg agcggggccg tgcggggcgg ggttgcgcgc
     1501 ctcgtgaccg atcgcctggc tctcgctctg tggaacgggc ttgggtttgc gggatggctt
     1561 gaaacgttgc ttgcgtggca tggctgggga gccagcaaca tccgtgcaca ggaccgggag
     1621 ttcatacgta gtatcgcgta ttcgattcgc tcccaatggt ccggtaacca caccaatgtg
     1681 gtcgtaccgt atgcctggat accgagctgt cctgcgaaat tgtgcgtgag aagcggcttg
     1741 ccagccttgc tctgtcatct tttgagggct cgccacctct ccacgagacc cgtatggccg
     1801 gatcggaact gcccgctcgg tgctgctgca gcgactggat cgccgggctc gcgcgcgatt
     1861 gcgccggacg gcttgcggcg atcgcccgac gagaaggcct gacagcgagc gacgcgctcg
     1921 acgtcgtcca ggacgcgttt cacaccctgc tccaccggcc ggacaccgcc gcgctgcgcg
     1981 accgacccga cgacgccgcg cgcctgctcg ccgcgatcgt gcgcaacgcc gccc
//
LOCUS       DASVOR010001294         2775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_42733,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001294 DASVOR010000000
VERSION     DASVOR010001294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42733"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF516_24685"
     CDS             <1..237
                     /locus_tag="VF516_24685"
                     /inference="COORDINATES: protein motif:HMM:NF036808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110962.1"
                     /translation="VRVEPDLTLSEPIEYPALFLKRGEDKRLREGHLWVFSNEVDVAR
                     SPRSSALDICQRVYQQRINVSPGAIGGAPGTASP"
     gene            complement(185..721)
                     /locus_tag="VF516_24690"
     CDS             complement(185..721)
                     /locus_tag="VF516_24690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8110963.1"
                     /translation="MTEPKPPTTEASAEELAVRAREGSRVSFDELVTRYRPRLVAFLA
                     RRLADPADAEDVAQETFLRAYDRLAQYDPSRPFATWLFAIGKNVAANHAIARNRRDAR
                     DRDSRPAGAAIEDGAGPSDLWQRAHAILSADAYRMLWLRYAQGLTVREIAAELDRSSV
                     AVKVMLFRARRRLLQEIR"
     gene            complement(749..1615)
                     /locus_tag="VF516_24695"
     CDS             complement(749..1615)
                     /locus_tag="VF516_24695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110964.1"
                     /translation="MLVVAILGVAGGLALATPFEPKMIPDQVQAVGHLDVDALRRTQL
                     FTAAGGQAAIDAALDQAPPKVRPVAHALAATLRGVSFWHSNEHGAVYLETRDAKSLGQ
                     LVAQLPATPGQPIDGFPTYTLTGHGTHFAGIYGDTLVVSDSEDGLQQSIRVLGGHAPS
                     LAGSRALPLSSRQGVFVFVTLGDHLLGAIQKTAHSKMLQLGLRSVVIDISEIAGQLTA
                     TAHAEMRSADAVQKATSMLEGLRAMASLSDDPRAQTLVDGVTVTGNGNAVEVVAKLPV
                     GEVVKAIHHHAK"
     gene            1733..2281
                     /locus_tag="VF516_24700"
     CDS             1733..2281
                     /locus_tag="VF516_24700"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HEX8110965.1"
                     /translation="MKKALVIVDIQNDYFPGGKMELHASEAAGARAGALLAHFRDRGL
                     PIVHIQHISARPGATFFLPDTDGVRIHASVAPAADETVIQKHFPNAFRDTRLLDHLRQ
                     HAIEELVIAGMMTHMCVDATTRAAADLGFRCSLASDACATRALSFGGTTVPADHVHTS
                     FLAALSGAYATVRPASELAVAE"
BASE COUNT          421 a          996 c          955 g          403 t
ORIGIN      
        1 gtccgcgtcg aaccggactt aaccttgagc gagcccatcg aatatccggc gctgttcctc
       61 aagcgcggcg aggacaagcg cctccgcgag ggacacctct gggtgttctc gaacgaggtc
      121 gacgtcgcgc gctcgccgcg atccagcgca ttggacatct gccagcgggt gtaccagcaa
      181 cgcatcaacg tatctcctgg agcaatcggc ggcgcgcccg gaacagcatc accttgacgg
      241 cgaccgacga gcgatcgagc tcggcggcga tctcgcgaac ggtcagaccc tgagcatagc
      301 gcaaccacag catccggtag gcatcggcgg acaggatcgc gtgtgcgcgc tgccacaggt
      361 cgctcgggcc cgcgccgtcc tcgatcgcgg cgccggccgg ccggctgtcg cggtcgcggg
      421 cgtcgcgccg gttgcgggcg atcgcgtggt tggccgcgac gttcttgccg atcgcgaaca
      481 gccaggtcgc gaacggccgc gacggatcgt actgcgccag gcgatcgtag gcgcgcagga
      541 aggtctcctg cgcgacgtcc tcggcgtcgg ccgggtcggc cagccggcga gcgaggaacg
      601 cgaccagccg cggccggtag cgggtcacga gctcgtcgaa cgagacccgg ctgccctcgc
      661 gcgcgcgcac cgcgagctcc tcggcggagg cctcggtggt cggtggcttc ggctcggtca
      721 tcacgcggtc atcacgtggc ccggcggctc acttcgcgtg gtggtggatc gccttgacga
      781 cctcgccgac cggcagcttc gccacgacct cgaccgcgtt gccgttgccg gtcacggtca
      841 cgccgtcgac cagggtctgc gccctcgggt cgtcggacag cgatgccatc gcgcgcagcc
      901 cctcgagcat gctcgtcgcc ttctgcaccg cgtcggccga gcgcatctcg gcgtgcgcgg
      961 tcgcggtcag ctgccccgcg atctcgctga tatcgatcac caccgatcgc agtccgagct
     1021 ggagcatctt cgagtgcgcg gtcttctgga tcgcccccag caggtggtcg ccgagcgtga
     1081 cgaacacgaa cacgccctgc cggctcgaca gcggcagcgc cctcgacccg gccaggctcg
     1141 gagcgtggcc gccgagcacg cggatcgact gctgcaggcc gtcctcgctg tcggacacca
     1201 cgagcgtgtc gccgtagatc ccggcgaagt gcgtgccgtg cccggtgagc gtgtaggtcg
     1261 ggaagccgtc gatcggctgc cccggggtcg ccggcagctg cgcgaccagc tggccgagcg
     1321 acttcgcgtc gcgggtctcg aggtacaccg cgccgtgctc gttgctgtgc cagaacgaca
     1381 ccccgcgcag cgtggcggcc agcgcgtggg cgaccgggcg gaccttgggc ggcgcctggt
     1441 cgagcgcggc gtcgatcgcg gcctgcccgc cggcggccgt gaacagctgg gtccggcgca
     1501 gtgcgtcgac gtcgaggtga ccgaccgcct ggacctgatc cgggatcatc ttgggctcga
     1561 acggcgtggc gagcgcgagc ccgccggcca ccccgaggat cgcgacgacg agcaacgcag
     1621 agaacgtaac gtggcgcatg gactctcctt tgcccgttgt acagcgccgg cgccgcgcgg
     1681 gttacatcgc ggtttgcgat acactcgccc gccccgaagg caggagcccg cgatgaaaaa
     1741 agccttggtt atcgtcgaca tccagaacga ctacttcccc ggcggcaaga tggagctgca
     1801 cgccagcgag gccgcgggcg cgcgtgccgg cgcgctgctc gcgcacttcc gcgatcgcgg
     1861 cttgccgatc gtccacatcc agcacatctc ggcgcgcccg ggcgccacct tcttcttgcc
     1921 cgacaccgac ggcgtccgga tccacgccag cgtcgcgccc gcggccgacg agaccgtgat
     1981 ccagaagcac ttcccgaacg cgttccgcga cacccggctg ctcgaccacc tccgccagca
     2041 cgcgatcgag gagctggtga tcgccggcat gatgacgcac atgtgcgtcg acgcgaccac
     2101 gcgcgcggcc gccgacctcg gcttccggtg ctcgctggcg agcgacgcct gcgccactcg
     2161 cgcgctgtcg ttcggcggca cgaccgtccc cgccgatcac gtgcacacct cgttcctcgc
     2221 cgcgctgagc ggcgcctacg cgacggtgcg gccggccagc gagctggcgg tcgccgagta
     2281 gctccgtcgc ttcccgatcg gacgctcgga gtgccgccgg gcaacgcgag cgagcagcgc
     2341 gtgcagcgcg gcgaacggcc gcggctcacg gtcgagcccg cctgacgcgg cgcgcacgat
     2401 cgctccgcat acgcggtgac cggaacacgc gcgccgctcc ggcgcgtcgc gccaccgctc
     2461 cgagctgtgc ggatcgctcg cgaatccgag cgaaccacct cgcggtgtac cggtacgttt
     2521 cgccatctcc gcgtgccgca aagcgaatac cacacaatgc cagatggttt tgcgttgcca
     2581 tcaagcatgt attgagatag tcattgctgg aggcggggga gcgaggccag cgctgggcat
     2641 tgccggcgcg catggacgcg ggcgatgaag cgcccggcga gctgccggac gtcatcacga
     2701 atgatcgcaa tgcacacagg aggagtcgcg tgaagagaaa gccattgcaa gtcaacctgc
     2761 atgcccgcga gtcca
//
LOCUS       DASVOR010001295         6023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_247263,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001295 DASVOR010000000
VERSION     DASVOR010001295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6023
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247263"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VF516_24705"
     CDS             complement(<1..1205)
                     /locus_tag="VF516_24705"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8110966.1"
                     /translation="MASNGHNGHTAGRGYFLETELAWGANATAAPWPDVRRGVALPAL
                     RESATGKPRLDVTAIFRGKNLMLIGSTGFVGKVALSMLLHRYPDVGRVYCLVRPGAGN
                     TADERFYKKVASSEVFDPVREVHGAAFEEFLRSKIVAVPGDIGRPLCNFSDEQFAEFD
                     RAGGLSAIINSAGLVSFAPSLESALRINAMGAKNVLDAARKAGARLCHVSTCYVAGRR
                     DGDVWEDEPVVGYFPRNEARDPVPRGGKRGTHGRPQGELLDRDFDPAAEIADCQRIID
                     QAREQSNDRQHISQFRERAAEALREQRRDPDDENDLKLAVARERKIWMNDVLTRLGME
                     RAEHWGWTNTYTYTKSLGEQIILSDRAVPATSVRPAIVESAVRYPFPGWNEGFTTTAP
                     LMYRMLKGHR"
     gene            complement(1222..2844)
                     /locus_tag="VF516_24710"
     CDS             complement(1222..2844)
                     /locus_tag="VF516_24710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HEX8110967.1"
                     /translation="MRAIRPKLRSHTREHIVTIDTDSAPRIMFYGENLMIEDLPVGTR
                     VIYPKRPMTPVANPKATIRYALNHPEDSPPLHALLRPGMKVTIAVDDISMPLPIMRTP
                     DVRQLVLEIVCEMLADHGVDDVHIVIALGLHRRMHDWEIKRMVGPKVWDEFWPDRLYN
                     HDGCDPDGMVVLGHAQGPHGHKELVEMNRRVAESDLTIYVNLNFVPMNGGHKSMAVGL
                     CGYESLKAHHTPHSIVESNSFMDPPRSFLAHSFKRQGDLIENTLKVFHIETALNNRAF
                     DGPLGFLTKNEDDFTEADRLKFQAMKWTLDKLPFAARREIFMRTPAAYELIACYAGAC
                     DPSHERTVAKLNEQNCVEVDGPADILLFGVPYISPYNVNSKALNPLLVQVMALGYFYH
                     MYRNQPILRDGGVLILTHPCSDRFDPVHHPSYIEFFNRILTETTDAYTIEQKYQDELA
                     YNPSYIEMYRRGNAYHGAHPCFMWYWGQRGREKTSRVIVVGADNATVPAIMGWETAGS
                     IAEAIAMARSTMGRSAQITMLHHPPYMISDVM"
     gene            complement(2881..3648)
                     /locus_tag="VF516_24715"
     CDS             complement(2881..3648)
                     /locus_tag="VF516_24715"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8110968.1"
                     /translation="MQPRPKYRNSAAFYDVDGTLIKINIVHAFAFYASRHASLAVSAK
                     NTVKTALSIPVFWAADKLSRKWFNEIFYRSYEGASEDRLVVLSEELFEDVIKPNIYPR
                     ARDLIEDSRRAGVRQVLVSGALDFTMRPLARYLGVDDLIANQLEFVDNYATGKLRKPF
                     VAGATKADIMRAYSREHGIDLAESWAYSDSFSDYPMLAVVGHPTACNPDLRLRSLARS
                     YDWPVLDLDEPADRSVARRPQGLAPVRRIRARPGQPE"
     gene            3714..4064
                     /locus_tag="VF516_24720"
     CDS             3714..4064
                     /locus_tag="VF516_24720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110969.1"
                     /translation="MRLPVWLTLGVALLVCVFGLYRIRIAFRSDEEDQRARERKGLYG
                     MGRRTHFLIGVIYLLLGAGLIATTFGWNPLGAMFGPRTERPGSGAAPSKPGSVPIDQL
                     PPPAQPAAPSAPGK"
     gene            4197..4778
                     /locus_tag="VF516_24725"
     CDS             4197..4778
                     /locus_tag="VF516_24725"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8110970.1"
                     /translation="MLDLELLKPERVIPRSRRLSRRAYDALVARGVFDDERIELLRGQ
                     LVTMSPQGGLHATVTARLAQRLVRALDESYDVRAHSPYAASDDSEPEPDISVSRRARR
                     LRYHPSKALLLIEVAESSLSKDRRVKAEIYAENRAPEYWIVDLASRSVFVHTDPGRGT
                     YRSVVQLSRKDVLRPVCLPGIALTVGELFRSSR"
     gene            4919..5491
                     /locus_tag="VF516_24730"
     CDS             4919..5491
                     /locus_tag="VF516_24730"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8110971.1"
                     /translation="MVPAAFDPPFLESQPLRLLSRREYDRLVDLGWFADEPIELLRGV
                     LVTMSPQGRPHASAVSFFNEQLVLQLGGRHEVRPQLPFAADDWSEPEPDLAVVPKDPG
                     LRDHPSEALLVVEIAASSLAIARGLKRTIYAEVGIPEYWIVDVNGRTVEVHTRPDGGY
                     YSRVHILRDGDVLRPTLLPAVAIRVADIPR"
     gene            5592..>6023
                     /locus_tag="VF516_24735"
     CDS             5592..>6023
                     /locus_tag="VF516_24735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110972.1"
                     /translation="MTKISIAFLAAMSLAGFGCHKNKGDADKAGAIAKLTELKNKMCA
                     CKDKTCSDKVSAELAAWGQEYDKPDVDKPASPDEKDSEKLEALKEETASCLLKIEVPS
                     GAGAPGAAAAGAAGGAGGAMDRGSPAAGSAGESAAAGSNTGG"
BASE COUNT          892 a         2066 c         2042 g         1023 t
ORIGIN      
        1 cggtggccct tgagcatccg gtacatcagc ggcgcggtgg tggtgaatcc ctcgttccat
       61 ccggggaacg ggtagcgcac cgcgctctcg acgatcgccg gccgcacgct cgtcgccggc
      121 accgcgcggt ccgacaggat gatctgctcg ccgagcgact tggtgtacgt gtacgtgttg
      181 gtccagcccc agtgctcggc gcgctccatc ccgagccggg tcagcacgtc gttcatccag
      241 atcttgcgct cgcgcgcgac cgcgagcttg agatcgttct cgtcgtcggg atcgcgccgc
      301 tgctcgcgca gcgcctcggc cgcgcgctcg cggaactgcg agatgtgctg gcggtcgttg
      361 gactgctcgc gcgcctggtc gatgatccgc tggcagtcgg cgatctccgc cgccggatcg
      421 aagtcgcggt cgagcagctc gccctgcggc cgcccgtgcg tgccgcgctt gccgccgcgc
      481 ggcaccggat cgcgcgcctc gttgcgcggg aagtagccga ccaccggctc gtcctcccac
      541 acgtcgccgt cgcgccgccc cgcgacgtag caggtcgaca cgtggcacag ccgcgccccg
      601 gccttgcgcg cggcgtcgag cacgttcttg gcgcccatcg cgttgatccg cagcgcgctc
      661 tccagcgacg gcgcgaacga caccagcccg gcgctgttga tgatcgcgga aagcccgccg
      721 gcgcggtcga actcggcgaa ctgctcgtcg ctgaagttgc acagcggccg gccgatgtcg
      781 ccgggcaccg cgacgatctt gctccgcaag aactcctcga acgccgcgcc gtgcacctcg
      841 cgcaccgggt cgaacacctc ggacgacgcg accttcttgt agaaccgctc gtcggcggtg
      901 ttgcccgcgc cggggcggac caggcagtac acccgcccga cgtcgggata gcggtgcaag
      961 agcatcgaca gcgcgacctt gccgacgaac ccggtcgagc cgatcagcat caggttcttg
     1021 ccgcggaaga tcgcggtgac gtcgagccgc ggcttgccgg tggcggactc gcgcagcgcc
     1081 ggcagcgcca cgccgcgccg cacgtcgggc cacggcgccg ccgtcgcgtt ggcgccccac
     1141 gccagctcgg tctccaggaa gtagccgcgg cccgcggtgt ggccgttgtg tccgttgctc
     1201 gccatgatca cccggccctg gtcacatcac gtccgagatc atgtacggcg ggtggtgcag
     1261 catcgtgatc tgcgcgctgc gccccatcgt gctgcgcgcc atcgcgatcg cctcggcgat
     1321 cgagccggcg gtctcccagc ccatgatcgc cggcaccgtc gcgttgtcgg cgccgaccac
     1381 gatcacccgg ctggtcttct cgcggccgcg ctggccccag taccacatga agcacgggtg
     1441 cgcgccgtgg tacgcgttgc cgcgccggta catctcgatg tagctcgggt tgtaggccag
     1501 ctcgtcctgg tacttctgct cgatcgtgta cgcgtcggtg gtctcggtca ggatccggtt
     1561 gaagaactcg atgtagctcg ggtggtgcac cggatcgaac ctgtccgagc acgggtgggt
     1621 caggatcagc accccgccgt cgcgcaggat cggctggttc cggtacatgt gatagaagta
     1681 gccgagcgcc atcacctgca cgagcagcgg gttgagcgcc ttgctgttga cgttgtacgg
     1741 cgagatgtac ggcacgccaa acaagaggat gtcggccgga ccgtcgacct cgacgcagtt
     1801 ctgctcgttg agcttggcga ccgtgcgctc gtgcgacggg tcgcacgccc ccgcgtagca
     1861 cgcgatcagc tcgtacgccg ccggcgtgcg catgaagatc tcgcggcgcg cggcgaacgg
     1921 cagcttgtcg agcgtccact tcatcgcctg gaacttcagc cggtcggcct cggtgaagtc
     1981 gtcctcgttc ttggtcaaga acccgagcgg gccgtcgaac gcgcggttgt tgagcgcggt
     2041 ctcgatgtgg aacaccttga gcgtgttctc gatcagatcg ccctggcgct tgaacgagtg
     2101 ggccaggaac gaccgcggcg ggtccatgaa cgagttcgac tcgacgatgc tgtgcggcgt
     2161 gtggtgggcc ttgaggctct cgtagccgca cagcccgacc gccatcgact tgtgcccgcc
     2221 gttcatcggc acgaagttga ggttgacgta gatcgtcagg tcgctctcgg cgacccggcg
     2281 gttcatctcg accagctcct tgtggccgtg cgggccctgg gcgtggccga gcaccaccat
     2341 gccgtcggga tcgcagccgt cgtggttgta cagccggtcg ggccagaact cgtcccacac
     2401 cttggggccg accatccgct tgatctccca gtcgtgcatc cggcgatgca gaccgagcgc
     2461 gatcacgatg tggacgtcgt cgaccccgtg atcggccagc atctcgcaca cgatctccag
     2521 cacgagctgg cgaacgtcgg gcgtccgcat gatcggcagc ggcatcgaga tgtcgtcgac
     2581 cgcgatcgtc accttcatcc ccgggcgaag cagcgcgtgc agcggcgggc tgtcctcggg
     2641 gtggttgagc gcgtagcgga tcgtcgcctt cgggttggcg accggcgtca tcggccgctt
     2701 ggggtagatc acccgcgtcc cgaccggcag gtcctcgatc atcaggttct cgccgtagaa
     2761 catgatgcgc ggcgccgagt cggtgtcgat cgtgacgatg tgctcgcgcg tgtggctgcg
     2821 cagcttgggg cggatggcgc gcatggttcc tttcgtcgat cctgtcggtc cgggtcgagc
     2881 ctactccggc tgtcccggcc gggcacgaat ccgccggacc ggcgcgaggc cctgcggccg
     2941 cctcgccacc gagcgatcgg ctggctcatc gagatcgagc accggccagt cgtacgagcg
     3001 ggccagcgag cgcaggcgca gatccgggtt gcacgcggtc gggtgtccga ccactgcgag
     3061 catcgggtag tcgctgaagc tgtcgctgta ggcccacgac tcggcgagat cgatgccgtg
     3121 ctcgcgggag tacgcgcgca tgatgtcggc cttggtcgcg cccgcgacga acggcttcct
     3181 gagcttgccg gtcgcgtagt tgtcgacgaa ctccagctgg ttggcgatca ggtcgtcgac
     3241 cccgaggtag cgcgcgagcg gccgcatcgt gaagtccagc gcgcccgaca ccagcacctg
     3301 gcgcaccccc gcgcgccggc tgtcctcgat caggtcgcgc gcccgcgggt agatgttggg
     3361 cttgatcacg tcctcgaaca gctcctccga cagcaccacc agccggtcct cgctcgcgcc
     3421 ctcgtacgag cggtagaaga tctcgttgaa ccacttgcgc gacagcttgt ccgccgccca
     3481 gaacaccggg atcgacagcg ccgtcttcac cgtgttcttc gcgctgaccg cgagcgaggc
     3541 atggcgcgac gcgtagaacg cgaacgcgtg gacgatgttg atcttgatca gggtcccatc
     3601 gacgtcatag aacgccgcgg aattcctgta ctttggccga ggttgcatcg ccgggagttg
     3661 gtgcactctg gcgcaggcgt cgcggcgctg tcaatggtag ggatgacata gccatgcggc
     3721 ttccggtctg gctcaccctc ggcgtcgccc tcctggtctg cgtgttcggc ctctaccgga
     3781 tccgcatcgc gttccgctcc gacgaggagg accagcgcgc ccgcgagcgc aagggcctct
     3841 acggcatggg ccggcgcacc cacttcctga tcggcgtgat ctacctcctg ctcggcgccg
     3901 gcctgatcgc cacgaccttc ggctggaacc cgctcggcgc catgttcggc ccgcgcaccg
     3961 agcgtcccgg ctcgggcgcc gcgccgagca agccgggcag cgtgccgatc gaccagctgc
     4021 cgccgccagc ccagcccgcc gcgccgagcg cgccgggcaa gtagaaggct ggctacccga
     4081 gcttcggaca cgcgtccgag cttcggacag ttcggacagc tgacgatgtg atctcgcgcg
     4141 gttcggctgg catgccgctc gctcagcgcg cgggccaaga tgctatggtc gaattcatgc
     4201 tggacttgga gctgctcaag cccgagcgag tcattccccg aagccgtcgg ctgtcgcgcc
     4261 gcgcgtacga cgcgctcgtg gcgcgcggcg tgttcgacga cgagcgcatt gagctcctgc
     4321 gcgggcagct ggtcaccatg agcccgcagg gagggctgca tgcgacggtg acggcgcgcc
     4381 tggcgcagcg cctggtccgt gcgctcgacg agtcatacga cgtccgcgcc cacagcccgt
     4441 acgcagccag cgacgattcg gagcccgagc cggacatctc cgtgtctcgc cgcgcgcggc
     4501 gcctgcgcta ccacccgagc aaggcgctgc tcctcatcga ggtcgccgag agctcgctga
     4561 gcaaggaccg ccgggtcaag gccgagatct acgcggagaa tcgtgcgcca gagtactgga
     4621 tcgtcgatct cgcatcgaga tcggtattcg tgcacacgga tccgggtcgc ggaacgtacc
     4681 gatcggtagt ccagctttca cgcaaggacg tgcttcgccc cgtgtgcttg ccggggatcg
     4741 ccctgaccgt cggcgagctg tttcgctcct cgcgctgact gcccgatcgg ctggcgactg
     4801 gagcttcgga cacctgtccg agcatcggac agttcggaca gccgatgatg cgatctcgcg
     4861 catttcggct ggcacaggac tggctacgtg ccgcccgcga gatgctatgt ttgactccat
     4921 ggtgcccgct gccttcgatc ctcccttcct cgagtcgcaa ccactccggc tgctatcgcg
     4981 ccgcgagtac gatcgcctcg tcgatctcgg ctggttcgcc gacgaaccga tcgagctctt
     5041 gcgcggcgtg ctggtcacca tgagcccgca gggccggccg cacgcctcgg cggtctcgtt
     5101 cttcaatgag cagctcgtgc tccagcttgg cgggcgccat gaagtccggc cacagctgcc
     5161 gttcgccgcc gatgactggt ccgagcccga gccggatctc gccgtcgtgc ccaaggatcc
     5221 gggcttgcgc gaccacccga gcgaggcgct gctcgtggtc gagatcgccg cttcgtcgct
     5281 cgccatcgcc cgcgggctca agcggacgat ctacgccgag gtggggattc cggagtactg
     5341 gatcgtggac gtgaacgggc gaaccgtcga ggtccacacg cggcccgacg gcgggtatta
     5401 cagcagggtg cacatcttgc gcgacggcga cgtgctgcgg ccgacgctgc tacccgcggt
     5461 ggcgattcgc gtcgccgaca ttccgcgttg atgcggctga cacttgcttg cctggagcca
     5521 ctgacggggt tccccttgca ggatatcctt cggacctcgc ttcttgcgaa cctcccgctg
     5581 gagtcgatcc gatgacgaag atttcgatcg cgttcctcgc tgccatgtcg ctcgccggct
     5641 tcggatgcca caagaacaaa ggcgacgccg acaaggccgg cgccatcgcc aagctcaccg
     5701 agctcaagaa caagatgtgc gcgtgcaagg acaagacgtg cagcgacaag gtcagcgcgg
     5761 agctcgccgc gtggggccag gagtacgaca agcctgacgt cgacaagccg gcgtcccccg
     5821 acgagaagga cagcgagaag ctggaggcgc tcaaggaaga gaccgccagc tgcttgttga
     5881 agatcgaggt ccccagcgga gcgggggcac ccggggcggc tgccgcaggg gcggccggcg
     5941 gggctggcgg cgcgatggac cgcggaagtc ctgcggcggg gtcggccggt gaatcggccg
     6001 ccgcgggcag caacaccggc ggg
//
LOCUS       DASVOR010001296         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421764,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001296 DASVOR010000000
VERSION     DASVOR010001296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421764"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            323..>1574
                     /locus_tag="VF516_24740"
     CDS             323..>1574
                     /locus_tag="VF516_24740"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein B"
                     /protein_id="HEX8110973.1"
                     /translation="MAGAHPEAAARFVADDDRLHWHDQAVWWVRQKRDTASAPVADWE
                     ALRTAAAGIKRHALSRLADYLEEFERNARAAGAQVHWARDAAEHNAIVHRLLADRGVT
                     RLVKSKSMLTEECKLNPYLEARGVEVVDTDLGERIVQLAHEPPSHIVMPAIHRKKEEI
                     GELFHVHLKTERGLADPTRLTEAARHHLRERFLAAGAGLTGVNFAVAETGAVVVCTNE
                     GNADMGTSLPPIHIACMGVEKIVPRERDLGVFLRLLARSATGQPITAYTTHYVGPRAG
                     GELHIVIVDNGRSEVLAREALRGSLACIRCGACMNTCPVFRRSGGHSYGYTVPGPIGS
                     VLAPLRDAARYATLPFASTLCGSCADVCPVRIDLHHQLLALRQDLARRALVPWQKRLV
                     LRVAALVLAWPAAYRLAGWLARRLV"
BASE COUNT          213 a          597 c          565 g          199 t
ORIGIN      
        1 cgtgctccgc cggcccgccg gtcccacccg ccatcccgtc cagcagcgtc cccgccacgc
       61 ccgacagcgc gagccactgg cgccagatct cgcccctgcg cggcccgccg accagcaccg
      121 tcacgcgcgg cgtgccgtgc aaccggaccc actggtcgcg cgcgagcctg cggagcaccg
      181 gaccgagcat cccgcgggag catgcgacaa ctcctgcatc ggctcaacgt ggcgagatcg
      241 cacggtgtca ggccttccgg gacccgtcac gcggaccggc aattcgactg cacatcgccg
      301 ggtttcggcc taccgtaccg ccatggccgg agcccacccc gaagccgccg cgcggttcgt
      361 cgccgacgac gaccgcctgc actggcacga ccaggcggtg tggtgggtcc gccagaagcg
      421 cgacaccgcg tcggcgccgg tggccgactg ggaggcgctg cgaacggcgg ccgccgggat
      481 caagcgccac gcgctgtcgc ggctcgccga ctacctggag gagttcgagc gcaacgcacg
      541 ggcggcgggg gcccaggtcc actgggcgcg cgacgcggcc gagcacaacg cgatcgtcca
      601 ccggctgctc gccgaccgcg gggtgacccg gctggtcaag tccaagtcga tgctgaccga
      661 ggagtgcaag ctgaacccct acctcgaggc gcgcggggtg gaggtcgtcg acaccgacct
      721 cggcgagcgc atcgtccagc tcgcgcacga gccgccgagc cacatcgtga tgccggcgat
      781 ccaccgcaag aaggaggaga tcggcgagct gttccacgtc cacctgaaga ccgagcgcgg
      841 gctggccgat ccgacgcggc tgaccgaggc ggcgcgccac cacctgcgcg agcggttcct
      901 cgcggcgggc gcgggcttga ccggcgtgaa cttcgcggtc gccgagaccg gcgcggtcgt
      961 ggtgtgcacc aacgagggca acgccgacat ggggacgtcg ctgccgccga tccacatcgc
     1021 gtgcatgggg gtcgagaaga tcgtgccgcg cgagcgcgat ctcggcgtgt tcctgcggct
     1081 gctcgcgcgc tcggcgaccg gccagccgat cacggcgtac accacgcact acgtcggccc
     1141 gcgcgccggc ggcgagctcc acatcgtgat cgtcgacaac gggcgcagcg aggtgctcgc
     1201 gcgcgaggcc ctgcgcggct cgctggcctg catccggtgc ggcgcatgca tgaacacctg
     1261 cccggtgttc cggcgcagcg gcggccacag ctacggctac acggtgcccg ggccgatcgg
     1321 ctcggtgctc gccccgctgc gcgacgccgc gcgctacgcg acgctgccgt tcgcgtcgac
     1381 cctgtgcggg tcgtgcgccg acgtctgccc ggtgcggatc gatctccacc atcagctgct
     1441 tgcgctgcgc caggacctgg cgcgccgcgc gctggtgccg tggcagaagc ggctcgtgct
     1501 gcgcgtcgcc gcgctggtgc tggcgtggcc ggcggcgtac cgcctcgccg gctggctcgc
     1561 ccgccggctg gtgc
//
LOCUS       DASVOR010001297         6888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_363970,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001297 DASVOR010000000
VERSION     DASVOR010001297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6888
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363970"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VF516_24745"
     CDS             <1..852
                     /locus_tag="VF516_24745"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8110974.1"
                     /translation="VRLRRRHQFRRLAILTSHLIAIVLGATATVIGGLHWAAWQPIRD
                     ARQGMPARTALGVPSHVDRILDATVVVLAPDADGDARNLAMGTGAVVFRDPGRAWIVT
                     CSHVAMPSVAVGTYHDVRTAPAVWVMLADGREGRATVRWVAAPPLDVALVELPIASSP
                     RPVEIAADTSGFGPDDSVTFVPNPFRSGWNVEHGKLVRRDTHHTTAGTYDLLVTDLRV
                     IPGDSGSGLYDVRGQLVGLNTWTSVEDGRARGISLPSEAMQQLVIAIRDGALDRLDQA
                     VGAPPRK"
     gene            857..1681
                     /locus_tag="VF516_24750"
     CDS             857..1681
                     /locus_tag="VF516_24750"
                     /inference="COORDINATES: protein motif:HMM:NF012406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-conjugating enzyme E2"
                     /protein_id="HEX8110975.1"
                     /translation="MENVRLRRLKADYDAMRRLAHMHPRIEIEGVFGNPPERYRLRLK
                     VKSLRERGEVIETVTEHRLEVTMPHGYPRDAPMFRMLTPVFHPNIAPHAVCIGDNWTA
                     GESIDQLIQRVGEILAYQSYNTKSPLNGRAAQWVDEHRDRLPIDPEEFFLDLSAAPPP
                     SEHAVPACVNCGAKTGLLAPCRAGHVSCGDCALPCPSCATIVCLTCGDRGCPVCAAAA
                     PRCANCGAKTGLLAPCRAGHVSCGDCAMQCPTCATLVCLACGDRGCRVCSAVAAPA"
     gene            complement(1701..4076)
                     /locus_tag="VF516_24755"
     CDS             complement(1701..4076)
                     /locus_tag="VF516_24755"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase"
                     /protein_id="HEX8110976.1"
                     /translation="MAILSGCSGDFDRTVISHDFDTAGPVDAGAEVDDAPGSDAGPPP
                     GGTLDPTAIPKYVTPLIIPPVMKHGGTPNVYRIAARQFKQQILPGGIWNTLNSRRDKY
                     PATTVWGYGPESDPVPDSSGLGGSIGVAPASNSQFNYPGYTIETLADVPVRVRWINGL
                     VDAHGNYLRHLLPVDQTLSWANPPMDCQVGPPRTDCAGDDPEPYIGPVPIVSHLHGAH
                     VDSHSDGYPEAWWLPAARNIPARFARSGSMFRDATEFNDGKRGYADYFYRQDQPATTL
                     WYHDHAMGITRLNVYAGLAGFWLIREGRHGRVSGTEPAVGPAARTFRVRGLPGPAAVA
                     GQTVLDLNVPGSPVRSGIREIPIVIQDRAFNQDGSLFYPSNRAFFERLNVAGTAGTPR
                     AQFPGAPELRIAFAPRSDMAPIWNPEAFFNVMVVNGVSWPKLEVAPARYRFRLLNGCD
                     ARFLNLALFPDRGPEIPVFQIGAEQGFLRRVVMISTGFATPLPGNGRRPAPVPAADPR
                     KALLMGPAERADVIVDFTGLADGTVITMVNTAPDAPFAGSLDDPADPGTTGHVMQFVV
                     NQHLRRPGDALAASPFDLRLEAEDPLGKTDRVRRVSLNEVDSAKVCVTQDAAGNVVQV
                     SGTPARGCPVGSAPFGPKEALLGVVDSTDPQNPRGIPLEFTDTTGISRPVPVSLRPGV
                     VVHVNVTENPKLGNTEKWEIYNFTPDAHPIHMHLVRFQVVGRRAMNGGPSVVGNAPQP
                     TESGFKDTVIAYPGEITTIKARFDLPGLYVWHCHILEHEDHDMMRPYVVSP"
     gene            4923..5339
                     /locus_tag="VF516_24760"
     CDS             4923..5339
                     /locus_tag="VF516_24760"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110977.1"
                     /translation="MRVLIVDDYPDTAETACELLRSAGHDCRSATCGAKALAVADAFT
                     PELAILDIGLPDIDGYVLRGALQSRLANCHPYFVALSGRTHALNAALAVGFDACLLKP
                     VGLAQLLRVVRLAQFENRGSPISKILLQRAEERSAG"
     gene            complement(5400..>6888)
                     /locus_tag="VF516_24765"
     CDS             complement(5400..>6888)
                     /locus_tag="VF516_24765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8110978.1"
                     /translation="KATSGTEALRRLLESDFAVVLLDVQMPEMDGYETARWIRSRDKT
                     RYLPIIFVTAHSHDDQSVLRAYKLGAVDFLFKPLNTDVLRAKASVFIELAQAQARDRE
                     HALREQRAQFENEALRVANEQLALADRHKNEFLAILGHELRNPLAPIRTSVDLLAHAA
                     DKPPSPRLLDILDRQLGHVTRLVDDLLDVSRINAGKLELRKQRVPFAEIIDEAVTICR
                     PFVDARQHRLSIIGDDGSMLVDADRTRLVQVLTNLLGNAARYTQKGGSIELSWGKDGE
                     HVFARITDNGIGIAAEVIGRVFDMFVQERAGSDGTGGLGLGLALAYQLAKLHDGTLTA
                     ASAGRGRGSVFELRLPIAEGTLVSSRAPRLEIASVQPLRAIVIDDDQDIRELTAELLA
                     SYGHQVMTADDGPSGLELICEQRPDVALVDLGLPGLDGCALARAFRERCPDGKTRLVA
                     MTGFGQDSDRRRARESGFDRHVVKPVGKAMLLAALIADEAAGATP"
BASE COUNT         1077 a         2382 c         2292 g         1137 t
ORIGIN      
        1 gtgcggctgc gccgccgcca ccagtttcgc cggctcgcga tcttgaccag tcacctcatc
       61 gcgatcgtgc tcggcgccac cgcgacggtg atcggcgggc tccactgggc ggcgtggcaa
      121 cccatccgcg atgcccggca ggggatgccg gcgcgcacgg cgctcggcgt gccgtcgcac
      181 gtcgaccgca tcctcgacgc caccgtcgtg gtgctggcgc ccgacgccga cggcgacgcg
      241 cgcaacctgg cgatgggcac cggcgcggtg gtgttccgcg atccgggcag ggcctggatc
      301 gtcacgtgca gccacgtcgc gatgccgtcc gtcgcggtcg gcacctatca cgacgtccgc
      361 accgcccccg cggtctgggt catgctcgcc gacggacgcg agggccgcgc caccgtgcgc
      421 tgggtggccg cgccgccgct cgacgtcgcg ctggtcgagc tgccgatcgc cagctcgccc
      481 cggccggtcg agatcgcggc cgacacctcg gggttcggcc ccgacgacag cgtgacgttc
      541 gtgcccaacc ccttccggtc gggctggaac gtcgagcacg gcaagctggt tcgccgcgat
      601 acccaccaca cgacggcggg gacctacgac ctcctggtca ccgacctgcg cgtgatcccc
      661 ggcgacagcg gcagcggcct gtacgacgtc cgcggtcagc tggtcgggct caacacgtgg
      721 accagcgtcg aggatggccg cgcgcgcggg atcagcttgc cctcggaggc catgcagcag
      781 ctcgtcatcg ccatccgcga cggcgcgctc gatcggctcg accaggcggt gggtgcaccg
      841 ccgagaaagt gacaccatgg agaacgtacg gctgcgccgc ttgaaggccg actacgatgc
      901 gatgcgccgc ctggcgcaca tgcatccccg gatcgagatc gagggcgtgt tcggcaaccc
      961 tccggagcgc tatcgcctgc ggctcaaggt caagtcgctg cgcgagcgcg gtgaggtgat
     1021 cgagaccgtc accgagcacc gcctcgaggt cacgatgccc cacggctacc cgcgcgacgc
     1081 cccgatgttc cgcatgctca cgccggtgtt tcaccccaac atcgcgccgc acgccgtgtg
     1141 catcggcgac aactggaccg cgggcgagtc gatcgatcag ctgatccagc gggtcggcga
     1201 gatcctcgcg taccagagct acaacaccaa gagcccgctc aacggccggg cggcgcagtg
     1261 ggtcgacgag catcgcgatc gcctgccgat cgatcccgag gagttcttcc tcgatctgtc
     1321 cgccgcgccg ccgccgagcg agcacgcggt ccccgcgtgc gtcaactgcg gcgcgaagac
     1381 cggcttgctc gcgccgtgcc gcgccggcca cgtctcgtgc ggcgactgcg ccctgccgtg
     1441 cccgagctgc gcgacgatcg tgtgcctgac ctgcggcgat cgcggctgcc cggtgtgcgc
     1501 ggctgccgcc ccgcggtgcg ccaactgcgg cgcgaagacc ggcttgctcg ccccgtgccg
     1561 cgccggccac gtgtcgtgcg gcgactgcgc catgcagtgc cccacgtgcg cgacgctggt
     1621 gtgcctggcc tgcggcgatc gcggctgccg ggtgtgctcg gccgtcgctg cgccggcctg
     1681 atggcggcgg tcgagccggc ctacggcgat accacgtagg gccgcatcat gtcgtgatcc
     1741 tcatgttcga ggatgtggca atgccagaca tacagcccgg gaaggtcgaa ccgggccttg
     1801 atcgtcgtga tctcacccgg gtaggcgatc acggtgtcct tgaagcccga ctcggtgggc
     1861 tgcggagcat ttccaacgac gctcgggcct ccgttcatcg cccgccgccc gaccacctgg
     1921 aagcgcacca gatgcatatg gatcgggtgc gcatccggcg tgaagttgta gatctcccac
     1981 ttttcggtat tcccgagctt cggattctcc gtcacgttga catggacgac cacgcccggc
     2041 cgcaggctca ccggaacggg cctgctgatc cccgtggtgt ccgtgaactc cagcggaatc
     2101 ccccgcgggt tctgcggatc ggtcgaatcg accactccca gcagcgcttc cttgggcccg
     2161 aagggagcac tgccgaccgg acaacccctc gcgggggtgc cgctgacctg gaccacgttc
     2221 ccggctgcgt cctgggtgac acagaccttg gcggagtcga cctcgttgag cgagacccga
     2281 cgcacgcgat cggtcttgcc gagcgggtcc tccgcttcca gcctcaggtc gaagggagat
     2341 gccgccagcg catctccagg ccgccgcagg tgctggttca ccacgaactg catcacgtgt
     2401 ccggtcgtgc ccgggtccgc cggatcgtcc agcgagcccg cgaacggtgc gtcgggcgcg
     2461 gtgttgacca tggtgatgac agtgccgtcg gccagtcccg tgaagtccac gatgacatcg
     2521 gcgcgctcgg ccggtcccat cagcagggct ttgcgcggat ccgccgccgg caccggtgca
     2581 ggtctcctgc cgttccccgg cagcggcgtg gcaaagcccg tcgagatcat gaccacgcgc
     2641 cggaggaagc cctgctcggc tccgatctgg aacaccggga tctccgggcc gcgatcgggg
     2701 aacagagcca ggttcagaaa gcgcgcgtcg cagccgttca gcagccggaa gcgatacctg
     2761 gccggagcga cctccagctt gggccagctc acgccgttga cgaccatcac gttgaagaac
     2821 gcttccgggt tccagatcgg cgccatatcg gagcgtggcg cgaaggcgat ccggagctcc
     2881 ggggcaccgg ggaactgtgc gcggggggtc cccgccgtcc ccgcgacgtt gagtcgctcg
     2941 aagaacgcgc ggttgctcgg gtaaaacagc gagccgtcct ggttgaacgc tcgatcctgg
     3001 atcacgatgg ggatctcgcg gatgccgctc cgcaccggac tgcccgggac gttgaggtcg
     3061 aggaccgtct gaccggccac cgctgccggg cctggtagcc cgcggacgcg aaacgtccgg
     3121 gccgcgggcc ccacagccgg ctccgtgccg gacaccctgc catgcctgcc ctcacggatg
     3181 agccagaagc cggccagacc ggcgtacacg ttgagacggg tgatgcccat cgcatggtcg
     3241 tgataccaga gcgtggtggc cggctgatcc tgccggtaga agtaatctgc atagccgcgc
     3301 ttaccatcat tgaattccgt ggcatcccgg aacatcgatc cactcctggc aaatctggcc
     3361 gggatattgc gggcagccgg caaccaccat gcttccgggt agccgtcgct gtgagaatcg
     3421 acgtgggcgc cgtgcaggtg actgacgatc ggaacagggc cgatgtacgg ctccgggtcg
     3481 tccccggcgc aatcggtcct gggcgggccc acctggcaat ccatcggcgg gttcgcccat
     3541 gacagcgtct ggtccaccgg cagcaggtgg cgcagatagt tgccgtgcgc atccaccagc
     3601 ccgttgatcc accggacccg caccggcaca tcggcaagtg tctcgatggt gtatcctgga
     3661 tagttgaact gcgagttgga agccggtgca actccgatgc taccgcccag gcccgacgag
     3721 tcgggcaccg gatcggactc cggcccgtag ccccacacgg tggtcgccgg atacttgtcc
     3781 ctccggctgt tgagcgtatt ccagatccca cccggcagga tctgctgctt gaactgccgt
     3841 gccgcaatgc ggtagacgtt gggtgtaccg ccgtgcttca tcaccggcgg gatgatcagc
     3901 ggagtcacgt atttcgggat cgcggtcgga tccagggtcc cacctggcgg gggacccgcg
     3961 tccgatccgg gtgcgtcatc aacctcggca ccggcgtcga cgggccccgc tgtatcgaaa
     4021 tcgtgagata tgacagttcg atcaaaatca ccgctgcagc cgctcaggat cgccacactc
     4081 actagccatc gattgcctgt catggccctc ctcctccaca tccaccgatg acaagcgtcg
     4141 ttgcctctcc gcccgcaccg atgacaagcg tcgttatcct cccgaccatg ctcacgaatg
     4201 cacactcggt gccacgtctg ttccagattc atgctccttt gattgcaacc acatctctgc
     4261 gatctccacg gccgtacaca cgtggcatcg ccgcacgacg agccgaaacg cactctgctc
     4321 gccgtgcgat cctcttcagg tgcttccgcg cggccgaatc ccgctgaagt ccgctcggtg
     4381 ccgctgagtc ccgtcgatcc tggcaagggc tggcaaagga tccgcgtcac ggatcgatgc
     4441 gggagatccc ggaccgggtt catttgcgcg tccggttctc ggcccatcgg gctcacgctg
     4501 tccggtcagc ggacgctccg ggggcaaccg gacaggcctg gcgcgctcgc ggtgatcgct
     4561 gccgccgact cgctgccgcc gcgacgtccc accaagcggg tgtgatcgcg gccagcggcg
     4621 atccggaacg cgacaaggac cacggcgaca agcgccgcgg tggcgccgga gatcccgctg
     4681 gcggcgagct ggagcagcgt cgtccccgca cgacgaagac gaggctcgcg ccaggtaacc
     4741 gcgtagccgc acgtctctgc gacgctcagt aaggcgagca ttggtctgca ggcgcgcctt
     4801 atgcaccgtg tgaggtggtg aggcatcata ctctgcccgg atcagtccgc cgcatcacgc
     4861 cacagagaat tacgtagaca ttcggagata cgcttctcga tccaaccacg agggaggcag
     4921 ccatgcgggt gctgatcgtt gacgactacc ctgatactgc ggagactgcc tgcgagctgc
     4981 ttcggagcgc ggggcatgat tgccggagcg caacgtgcgg cgccaaggcg ctcgccgtgg
     5041 cggatgcgtt cacgcctgag cttgcgatcc ttgacatcgg tctcccagac atcgacggct
     5101 acgtactacg gggggcgctc cagagccggc ttgcgaactg tcacccctat ttcgtggcac
     5161 tgagcgggcg gacccacgcg ctcaacgcgg cgctcgcggt tgggtttgat gcgtgcctgc
     5221 tgaagccagt tggcctggcg cagctcttgc gggttgtccg gctcgcgcag ttcgaaaacc
     5281 gcggctcccc gatcagcaag atactcctcc aacgcgcaga agagaggtct gcgggctgag
     5341 cgctttctct gcaccctcga ccagctcgat ccctcatagg tcgacatgct cgttgcagat
     5401 cacggcgtcg cccccgcagc ctcgtccgcg atgagtgctg cgagcagcat cgccttgccc
     5461 accggcttga ccacatggcg atcgaagcct gactcgcgtg cgcgccgccg atcgctgtcc
     5521 tggccaaaac cggtcatcgc gacgagccgc gtcttgccgt cggggcagcg ctcgcggaat
     5581 gcacgtgcga gcgcgcaccc gtcgagaccg ggcagcccga gatcgacgag cgccacgtcc
     5641 ggccgctgct cacagatgag ctcgagaccg ctcggaccat cgtccgcggt catcacctga
     5701 tgtccatacg aggcgagcag ctcggccgtc agctcgcgga tgtcctggtc gtcgtcgatg
     5761 acgatcgcgc gcagcggctg gacgctcgcg atctcgaggc ggggtgcccg cgaggaaacg
     5821 agcgtgccct cggcgatggg cagccgaagc tcgaacacgc tgccacgtcc gcgcccggcg
     5881 ctcgcagcgg tcagcgtgcc gtcgtgcagc ttcgccagct gatacgcgag cgcgaggccg
     5941 agccccaggc caccggtccc gtcgctgccc gcgcgctcct ggacgaacat gtcgaacacg
     6001 cgcccgatga cttcggctgc gatgccgatg ccgttgtcgg tgatgcgcgc aaagacgtgc
     6061 tcgccgtcct tgccccacga cagctcgatc gagccgccct tctgcgtgta gcgggcggcg
     6121 ttgcccaaca ggttcgtgag cacctgcacg agccgggtgc gatcggcgtc cacgagcatg
     6181 ctgccgtcgt cgccgatgat cgagagccgg tgctggcgcg catcgacgaa cggccggcag
     6241 atcgtcacag cctcatcgat gatctccgca aatggaaccc tctgcttgcg cagctccagc
     6301 ttgccggcgt tgatgcgcga gacgtcgagc agatcgtcga cgagccgggt cacgtggccg
     6361 agctggcgat cgaggatgtc gagcaggcgc ggcgatgggg gcttgtccgc tgcgtgcgcg
     6421 agcaggtcga ctgatgtcct gatcggcgcg agcgggttgc gcagctcgtg gccgaggatc
     6481 gcgaggaatt cgttcttgtg gcggtcggcg agcgccagtt gctcgttggc gacgcgcagc
     6541 gcttcgttct cgaactgggc gcgttgctcg cggagcgcgt gctcgcgatc gcgtgcttgt
     6601 gcttgcgcca gctcgatgaa caccgacgcc ttggcgcgca gcacgtcggt gttcagcggc
     6661 ttgaacagga agtcgaccgc accgagcttg tacgcgcgca ggacggactg gtcgtcgtgg
     6721 ctgtgcgcgg tcacgaagat gatcggcagg tagcgcgtct tgtcgcgcga gcggatccac
     6781 cgcgcggtct cgtagccgtc catctcgggc atctggacgt cgagcaagac gacggcgaag
     6841 tcactttcga gcagcctgcg gagcgcctcg gtgcctgacg tcgccttg
//
LOCUS       DASVOR010001298         4025 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_275613,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001298 DASVOR010000000
VERSION     DASVOR010001298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275613"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VF516_24770"
     CDS             <1..835
                     /locus_tag="VF516_24770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8110979.1"
                     /translation="GKELIAREIHNRSPRAGKPFVTINCGAIPENLLESELFGHVKGA
                     FTGAIANKQGKFQAADGGTLFLDEIGEMPIELQVKILRALQERVVYRVGDTRPETCDI
                     RILAATNRDLDKEIAAGRFREDLYYRLNVVNVDLPPLRQRGEDVLVIARYLLSRYARE
                     YDVKVKGLSPNAAVAIRKHAWPGNIRELENRIKKAIVLCESSVIGPDDLGLTGDVLPQ
                     ILTLAEAKDKFQRDYINEVLALNNGNRTKTARDLGVDPRTVFRHLEKESEDGDVGVDV
                     T"
     gene            974..1489
                     /locus_tag="VF516_24775"
     CDS             974..1489
                     /locus_tag="VF516_24775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110980.1"
                     /translation="MAIAGAVLLCMLVATLVGCVIPPSLRVDEENNSPPVITSVSGDS
                     SMLADPGPVVVEAGTTSSNLVLTLLDTDDRDDLHVRLFVDYNAPDRLPARVACDVPHN
                     DSNPQAKRTATCNLSGLCLTSDIGMQRGLMLVVFDRPPPDFGADPQAIAAPGLSSYRF
                     FFLRCQPPQTP"
     gene            1576..3354
                     /locus_tag="VF516_24780"
     CDS             1576..3354
                     /locus_tag="VF516_24780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110981.1"
                     /translation="MIASIVALGGCLSAPEGVAPMCHTDNDCDRSHGEVCEEGICWGD
                     PPPGPYAAVVSPPSSRQDLVAREIPQLAIPDFGWLGDLALEAPVLLSGKLVAFCPPPL
                     VACDPTPLAGTVTVSRPPQFRGGPGVRATVNVAAGGSFAIPVRRTQQGDDPYTVTLVP
                     DATRLATGRSAAEIVPPRRMQISISDNLATQAFELGGADLPTISGTLRDSSNAGIASY
                     RISAFGHWDPTEPAVEVSTVDYTDASGAFSVTLSDELVGTVELVARPVSATFMGAPPT
                     AAVIHVSGLDATMPSTRDIVVPNTLGKPATQTLQIQGLDDNGTITPVSGAQVSITGTL
                     TDSLTAFTVTDTEIADAGGQVTLKVLDGDALTSSYRVSVTPPAGSSLGGVIFDQKLSS
                     FPMLPQVRLTPRLALRGKLFYGGKPLANATITARPSLRFLWALDATAQVFVASIPPST
                     FTIPTNGGDFVLKVDPNVAQIWGYYDLVIEPAAGTRAPSFVLPEFAIPRNPALDPVTV
                     TYNLPEPGAAPGIYLPEAAFIHGRITGPDGHVVEGAELKIYQVSTQLTLCSEVAHAPA
                     SCPIPAALEARNTSDAEGTVRLALPH"
     gene            3641..>4025
                     /locus_tag="VF516_24785"
     CDS             3641..>4025
                     /locus_tag="VF516_24785"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8110982.1"
                     /translation="MTDDTVPHQNGVADDLAAIAAPPPAPAPSPEPELPPEAAAASPE
                     AAPAAPETPETPAEPPPPSFDDLGLHPDVKLALDEMGDLLPTPVQTAVYRPVSEGKDL
                     LVQSRTGTGKTTAFGLPTISKIIPTH"
BASE COUNT          628 a         1516 c         1301 g          580 t
ORIGIN      
        1 cggcaaggag ctgatcgcgc gcgagatcca caaccgctcg ccgcgggccg gcaagccgtt
       61 cgtcacgatc aactgcggcg cgatccccga gaacctgctc gagtccgagc tgttcggcca
      121 cgtcaagggc gcgttcaccg gcgcgatcgc caacaagcag ggcaagttcc aggccgccga
      181 cggcggcacg ctgttcctcg acgagatcgg cgagatgccg atcgagctgc aggtcaagat
      241 cctgcgcgcg ctgcaggagc gcgtggtcta ccgggtcggc gacacccggc ccgagacctg
      301 cgatatccgg atcctcgccg cgaccaaccg cgacctcgac aaggagatcg ccgccggccg
      361 gttccgcgag gacctgtact accggctcaa cgtcgtcaac gtcgacctgc cgccgctgcg
      421 ccagcgcggc gaggacgtgc tggtgatcgc gcgctacctg ctgtcgcgct acgcgcgcga
      481 gtacgacgtc aaggtcaagg gcctgtcgcc caacgccgcg gtcgcgatcc gcaagcacgc
      541 ctggccgggc aacatccgcg agctggagaa ccggatcaag aaggcgatcg tgctgtgcga
      601 gtcgagcgtg atcggccccg atgacctcgg gctgaccggc gacgtgttgc cccagatcct
      661 cacgctcgcc gaggccaagg acaagttcca gcgcgactac atcaacgagg tgctcgcgct
      721 caacaacggc aaccgcacca agaccgcgcg cgacctcggc gtcgacccgc gcaccgtgtt
      781 ccgccacctc gagaaggagt ccgaggacgg cgatgtcggg gtggacgtga cctagctcac
      841 aaaccgcagt ggacacgcca tgacgagaat gtccgatcca cgcagccgca cctccgcccc
      901 gcccgcctgt aacccctggc aatcccgggg aggatcgggg ctggcgcgcg cagtgcaagc
      961 agctcgagct gccgtggcga tcgcaggagc cgtgctgctg tgcatgctgg tcgcgacgct
     1021 cgtcggttgc gtcatcccgc catcgctgcg cgtcgacgag gagaacaact cgccccccgt
     1081 gatcacctcg gtcagcggcg acagctcgat gctcgccgat cccggcccgg tggtcgtgga
     1141 ggcaggcacc acctcgagca acctcgtgct cacgctgctc gacaccgacg atcgcgacga
     1201 ccttcacgtc cggctgttcg tggattacaa cgcccccgac cggctgccgg cccgggtcgc
     1261 gtgcgatgtg ccccacaacg attccaatcc ccaggcgaag cgcacggcga cctgcaacct
     1321 gtcggggctg tgcctgacgt ctgacatcgg catgcagcgc ggcctgatgc tcgtcgtctt
     1381 cgatcgccca cccccggact tcggggccga tccgcaggcg atcgccgcgc cgggcctgtc
     1441 gagctatcgg ttcttcttcc tccgctgcca gccgccgcaa acgccatgac cgcacctccg
     1501 agagctcctc gtctcctgcc cgccgccgct gccgctgcat gggccgcgtc gcttgctgcg
     1561 tcgatggtcg cgtcgatgat cgcgtcgatt gtcgctcttg gcggctgcct cagcgccccc
     1621 gaaggcgtcg cgccgatgtg ccacaccgac aacgactgcg atcgatcgca cggcgaggtc
     1681 tgcgaggagg gcatctgctg gggcgaccca ccgcccggtc cgtacgccgc ggtggtgtcg
     1741 ccgccgagct cgcgccagga tctggtggcg cgcgagatcc cgcagctggc gatccccgac
     1801 ttcggctggc tcggcgacct cgcgctggag gcgccggtgc tgctgagcgg caagctcgtc
     1861 gcgttctgcc cgccgccgct ggtcgcctgt gatccgacac cgctcgccgg caccgtcacg
     1921 gtgtcgcgac cgccgcagtt ccgcggcgga ccgggggtca gggcgaccgt caacgtcgcc
     1981 gccggcggct cgttcgcgat cccggtgcgg cggacccagc agggcgacga tccgtacacc
     2041 gtcacgctcg tccccgatgc cacccggctg gccaccggcc gcagcgccgc ggagatcgtc
     2101 ccgccgcgcc ggatgcagat ctcgatcagc gacaacctcg cgacccaggc gttcgagctc
     2161 ggcggcgcgg acctgccgac gatcagcggc acgctgcgcg acagctcgaa cgcgggcatc
     2221 gccagctacc ggatctcggc gttcggccac tgggatccga ccgagccggc ggtcgaggtc
     2281 tcgacggtcg actacaccga cgcgagcggc gcgttctcgg tgaccctgtc cgacgagctg
     2341 gtcggcaccg tcgagctcgt cgcccggccg gtgtcggcga cgttcatggg cgcgccgccg
     2401 accgccgcgg tgatccacgt cagcggcctc gacgcgacga tgccgtcgac cagggacatc
     2461 gtcgtgccga acaccctcgg caagccggcg acgcagaccc tgcagatcca ggggctcgac
     2521 gacaacggca cgatcacgcc ggtcagcggc gcgcaggtct cgatcaccgg cacgctgacc
     2581 gactcgctga ccgcgttcac cgtgaccgac accgagatcg ccgacgccgg cggccaggtg
     2641 acgctcaagg tgctcgacgg cgacgccctg acgagctcgt accgggtgtc ggtcacgcca
     2701 ccggcgggct ccagcctcgg cggggtgatc ttcgaccaga agctgtcgtc gttcccgatg
     2761 ctgccgcagg tccggctcac cccgcggctc gcgctgcgcg gcaagctgtt ctacggcggc
     2821 aagccgctcg ccaacgccac gatcacggcg cggccgtcgc tgcgattcct gtgggcgctc
     2881 gacgcgaccg cccaggtgtt cgtcgcgtcg atcccaccgt cgaccttcac catcccgacc
     2941 aacggcggcg acttcgtgct gaaggtcgat ccgaacgtcg cgcagatctg gggctactac
     3001 gacctcgtga tcgagcccgc ggccggcacc cgcgcgccca gcttcgtcct gcccgagttc
     3061 gcgatcccgc gcaaccccgc gctcgatccg gtcaccgtca cgtacaacct cccggagccc
     3121 ggcgcggccc ccggcatcta cctgcccgag gccgcgttca tccacggtcg gatcacgggg
     3181 cccgacggcc acgtggtcga gggcgccgag ctcaagatct accaggtctc gacccagctg
     3241 accctgtgta gcgaggtggc gcacgcgccg gcgtcgtgtc cgatccccgc tgcgctcgag
     3301 gctcgcaaca cctcggacgc cgagggcacc gtccggctgg ccctgcccca ctgaccgatc
     3361 cgtggccggg tttcgccttg accggggcag acctggcgac taaggtctgc gcaacgcaag
     3421 ggtctcgtgt tcgagtcagg aaaaaacagg gtttgcaggc gcgatgtgcg tcgcgagctc
     3481 tgtcctgtgg gagtcccgtg gagcagccga ggacgacgtc gtccgcccgc tgcgaatctc
     3541 cacgccaaca cttcccggcc agcgctccga ctgggtgatc tcggagcatc acgcacatcc
     3601 tcgggcttgg cccctggatt ccctccgagg gatttggacg atgacagacg atacggtgcc
     3661 ccaccagaac ggtgtggccg acgaccttgc cgcgatcgcg gcaccgcctc ccgctccggc
     3721 cccatctccg gagcctgagc ttccccccga agccgcggcc gcgagcccag aagccgcccc
     3781 cgcagctccg gaaacccccg aaacaccagc tgaaccaccc ccgccgagct tcgatgacct
     3841 cggcctgcac cccgacgtca agctcgcgct cgacgagatg ggggacctgc tgccgacccc
     3901 ggtccagacc gcggtgtacc ggccggtcag cgagggcaag gacctgctgg tccagtcgcg
     3961 caccggcacc ggcaagacca cggcgttcgg cctgccgacg atcagcaaga tcatcccgac
     4021 gcacc
//
LOCUS       DASVOR010001299        13867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_334610,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001299 DASVOR010000000
VERSION     DASVOR010001299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334610"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..7201
                     /locus_tag="VF516_24790"
     CDS             2..7201
                     /locus_tag="VF516_24790"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEX8110983.1"
                     /translation="MTRKTDKGLPRYIDAEESDVFVLSGAEDLVPLQTEPVSRDGYRI
                     QRYRPRVEGLFARIERWTNQTTGDSHFRILSRDNATSIYGRSASARIADPAAPSRVFS
                     WLLEETRDDRGNAVRYTYKAEDGAGVAPTKTSEVRRFEQAPGAPRFLATAQRYLQRIQ
                     YGNRAPLGDHEPAPADPSAWLFEVVFDYGEHDATAPTPDDELRPWQLRADPPSSFRAG
                     FEVRTYRLCRRVLLFHRFAELGSTPCLVRSTDFTYDESPALTYLVKVEQAGYVRRADG
                     SYTRAALPPLELDYARLTELDDTLQILDRASLDGIPEGIGGGTTQWVDLDGEGIPGVL
                     RADERGWYYKANLGDGRLAPPALLRSLPIPAELSGGFQQLVDLQGDGRLELVHYSQPL
                     AGYFTRTADGSWEPFTALRSVPSIDWGDANLRTLDLDGDGFPDVLITEHDAFVWYRSL
                     ATDGFEPARRVGRILDEEKGPALVFADGTESIYLADMGGDGLVDIVRVRNGDVSYWPN
                     LGYGQFGPKVTMDGGPQFDRGDRFDPKRIRFADVDGSGTSDILYLGRDGVAIYLNRAG
                     NSLSEPQVIRSLPVVDTLAGTTVTDLRGRGTACLVWSSPAPAREGRPIVYVDLMNGVK
                     PHLLVSTKNNLGAETRIDYASSTQFYLADKAAGTPWITRLPFPVHVVERVTTYDRISR
                     NRFVTRYTYHHGYFDGVEREFRGFGRVDQLDTEELAALTASGDFPAADNIDAASYVPP
                     VLTRTWFHTGAFIDRDRISTQLAQEYHVEPGLADEQRRAMLLDDTVLPDGLSADDVRE
                     ACRALKSSILRSEVYALDGTEAEGRPYLASEHSYAIVLVQPRGNNKYGVFFVHPREAI
                     EFHYERKLYDVGGTKLADPRVTHALTLEVDDFGNVLRSVAIGYGRRHDSPDPVLTAED
                     RQRQRQLQITCNENAYTNAIDQDNAYRTPQPCESRTYEITKLSPSASLPDVTNLFGFD
                     EMLQAIDGLEDGAHDLAYEDLAGAGATQAHPYRRLVEHMRTLYRRDDLTGPLAFHHLE
                     SRALPYESYKLALTPGLLTSVYGDRVTDAMLAGDGGYVHSQGDANWWIPSERAFYSPG
                     KDDTAAQELAYAIQHFFLPCRFRDPFGHTTALTYDSYDLLALQTRDALDNLVTIGARD
                     AAGAIVDNGNDYRVLQPVRIMDPNRNRKAVAFDALGMVVGTAVMGKPDEHVGDSLDGF
                     VADLPGSAIAAHLADPLADPHAILQRATTRLVYDLLAYQRTQADLRPQAPVVYTLARE
                     THDANLAPGEKTKVQHSFSYSDGFGREIQKKMQAEPGPLVPGGPILGPRWVSSGWTIF
                     NNKGKPVRQYEPFFTDTHGFESSKIHGVSSVLFYDPAERVVATLHPDHTYEKVVFDPW
                     QQASWDVNDTVLQTDPVADPDIGGFIARLPAEEYLPTWYERRSGGALGTEERDAADKA
                     AAHADTPAAAHFDAVGRQFLTVAHNRFKRADIVIEEKYRTMSILDIEGNQRAVIDAKD
                     RVVMRYDYDLLSNRIHQASMEAGERWMLGDVLGKPIRVWDSRGFLRRMAYDELHRPTG
                     LYVTENGGERLAERTVYGEAQGDTANHRGQVYQRFDAAGIMTSTGYDFKGNLGETRRD
                     LLPTYKVAVNWLQNPAATDGSFATSTMFDALNRPLAVTSPDGSIYQPVFNEASLLTNV
                     NVRLRGAFTATIFVTNINYNAKGQRERIDYGNGVSSTYEYDLFRSRLVHLVTTRPTSP
                     DATASQLFQDSSLVQDLHYSYDSAGDITRIEDAALKTTFHDGQEVKPVGTYTYDAVYR
                     LIEATGREHISQTAFDFNPSDGNYRDYPFAGSRAHLNDLQALCNYIELYEYDPVGNFE
                     TLAHRANGAGWTRRYDYQDNSLLEANKMSNRLTRTMVGDGLDRAESYTYDAHGNMTSM
                     PHLAAMAWNFKDELQRADLGGGGAAYYVYDGTGQRVRRVIESQNGMRQKERLYFGGFE
                     IYREYSGDGITINLERESLHVMDGKQRIALTEAQTIQERNLVKAPDPVLRYQLGNHLG
                     SASVELDSNGSLISYEEYSPYGTTSLQAGRSIAEIGLKRYRYTGKERDNETGLYYNEA
                     RYFACWLVRWTTVDPSGPVDGLNLYEYCRSNPIIFHDPDGHAIEGYYIARGRSGNDLP
                     SLFLGISAHVLIAKDYERKHGNDLSFYNYKTLRTILEISRAGDPKNLKVEDQHLKPDI
                     TNTTIREVFEAKPWNKQGIKKGWKEVNRYLAGLNDAAPKNAQFKLGTGHSGEILVRFA
                     NGVPGWKLSWQTTVPGVIQYRWQTLNRKRRSEEFQRETYQQAQWVALSRAEAAQYSEQ
                     LYRGIEKHIENEEKRPVTRLPFFMTLGIHLLLMNSVTRRLLGGSRTRSEESALATPPA
                     ADSQQGLQPPSYLQQGQRPSQRPLPSGPICPVPSQ"
     gene            7299..7736
                     /locus_tag="VF516_24795"
     CDS             7299..7736
                     /locus_tag="VF516_24795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110984.1"
                     /translation="MSITLDTDRFSLANLKQLIEMNPSIDWGAVVLDDPGRRAAIDWR
                     ELDPETMVPLVKAYQRLMHILPPGEERRALPLLESGLHSAIQIASIPRDEFLRRWGAL
                     FPGDEALGLAVHRAALGRRSELVLHHIHEVQRNEPHYRAARFK"
     gene            7755..>13867
                     /locus_tag="VF516_24800"
     CDS             7755..>13867
                     /locus_tag="VF516_24800"
                     /inference="COORDINATES: protein motif:HMM:NF015501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemopexin repeat-containing protein"
                     /protein_id="HEX8110985.1"
                     /translation="MKSSFTSLFGPIDHAEGNESRSVLSPAAYLVDLLELRDSIVTGW
                     TDYHARRPDVRGIHLDQASTFTEIPFLDVANAVMGAATRQLVGGTEDVDTVVGRVLTG
                     TLFPPPLPFSEQHLRLQLYAGKLGTSLDEIQRLYRASADAHLSARLRLGLGPEEYALF
                     STAHDSDDGIKKLWGVSDLAALDGSDVGGIEQKLGLSRKELKQLVRQDLSAAELSEPR
                     DPSKPKRNAAESFFINQGPSYLVLDEAPLKLHLKDGSKLSNQHLDRMMRFVRLARRLD
                     LELADLDWLLQTACGNVLDGDALQAIAIALDLRKATGLPIDEICSLWSVAKDHGQGDG
                     PIPADLFDRAYNNDVPITLEALVAGLAAGATVDALDDRLQATLRVSGADFAFLKAALL
                     ARGVARPTLAYPAIVAWFSAFRRIATLATMLALSVREVVMLLDVLGAQWTVSEHEDLS
                     IAMPFSAAALPTTPLGLLVQPDAPPRRTMDVVQKLFRVKAWLDLRQLSARQLAYLCLE
                     DHLDARQRLDDGPPIDDVQPDDAVEAALTDLQQSLLDAMVVPSALQTGSLTADGARAV
                     FDALRDARVLVTFDDPARGLLRVQPSDGELAAAMQSGIHERLEVHASDFAALGLTDAD
                     PLFSLLVAHGYLDRREETVEGELEVHHVIAPGLDGYFASPGGAATFTLPSFQAHAEAI
                     FSVLAQRVDAARVDAAALAATGIDGADVPALFAVLQAHGYAEPVPGSTPIDYRVLVDA
                     KPFFSDPANLAKFALPDFTTAFAVLAAKVAAFQRGMANLPTEAQELGGRLSASSDQQI
                     RAWLRSLSGLIGLAEDVTALAFAWAFGTPDETMTQTLAALALPLFRARQQSQPALGDA
                     YLASRCRRLQQLALLLRKTAMTADQARVYLTNQQVHRRLPETLNLPDGLLSSGKIDAL
                     ATLPGGDLVVISGPTYAQFSQHDDHLVGTGTLDQIPGVALPDAFRARVRASGIDAAFN
                     DNGALQLCAGDQYVTVSAAGTSAAKPISDWGQVRNNLQQHARVDAAVQAKDGRLFLFS
                     GDQYVLYSAPQQLLAGPAFVDERYPRSIRGKFENEGVSPLPALMFSGVDAAFRDTDDT
                     YYFFAGNRFSHSTDPFNVVKIRPVWGHVLNDLFEDNRVDAAFVLGGVTYLTRRNQLLR
                     YTGSAYQIVDEGFPISFGNVADSEPLLRVLRRFPNGLDAALAGSDGKLYAFKDGAYAS
                     SAAPDTSLTIRDHWGRVRNVFVDNQRVDAALAYGGAVYLFSGDQYVRYSGASYQLVDE
                     GYPRRVRPSWNALEKIGVVPDGLPLPITAVAVGRAPGGTSDDVYFFAGNQFAGPTGAL
                     DDIKARWARVRNNLGKVGVVDAAMLDGSGRMYLFSGDQYYRYSSPDQTFVDETYPRRL
                     AGNWVQEGSGYALPDAFAGGISAALRTTDGTLYFFSGQSYARVDTAPATPRANNQDWG
                     VVRNVIQAQNAVGAAYVDPAGKTYVLGGDQVVRYTGTAYDVVDEGFPLAIATRWPSLP
                     ASFRSDIDAALVFKSPVDNVQRLYLFKGDSYCRFSTGDFTQLDAGYPRRYLDAIQAEG
                     LWFRGLAVHEPNPNSSHQDDPIASVEAIYVDTFGGQPRINVFYRHDGGIQWRRIFQSG
                     NWGAPVRVETLSDYLPFTRLDAALVATDGTLHAFSGNQYASRPPAGGPLTAPVPINSR
                     WARVFNQFADLGRVDACLTMGDGRTYLFCARQFIKYTGTLRPGAADFFVDEGYPLRID
                     PNWPAQGVPVTLAPELQAAGYDLCRDATGKVHFFNGTRYAVSGTAGDVLLTSRWGKVE
                     NRFQDLDRVDAAYRAENNKLYLFCDTQYTRYSGALQPGSPDFYADEGYPRHVTTGWAS
                     EGLPIALPAVWNALGSAIFRDAQQTYVFSGSSFTSSQSPAPAPVIPQWAKVRNQIQSQ
                     NRVDAGFVLGQGAGAVTLVFCDDQYVRYSGAYDGFVDEGYPKVIAHLAAADGAFPGLP
                     DPLPSGLRGFFAGTDGNLHAFGPQPSDASQPQLYVSSAGPATLRPLDQRWGIVDNKLW
                     DNEFVNAALVAGGKLFLFSGD"
BASE COUNT         2433 a         4735 c         4451 g         2248 t
ORIGIN      
        1 gatcacgcgc aagaccgaca agggccttcc gcggtacatc gatgccgagg aatccgatgt
       61 cttcgtgctg tccggagccg aggacctcgt gcccctccag accgagccgg tctcgcgcga
      121 cggctatcgc atccagcgct atcggcctcg cgtggagggg ctgtttgcgc ggatcgagcg
      181 ctggacgaac cagaccaccg gagacagcca cttccggatc ctctcgcggg acaacgcgac
      241 cagcatctat ggcaggagcg cgagcgcgcg gatcgccgat ccggcggcgc cgtcccgggt
      301 gttctcctgg ctgctcgagg agacgcgcga cgatcgcggc aacgccgtgc gctacaccta
      361 caaggcggag gacggcgcgg gggtcgcccc gacaaagacc agcgaggtca ggcgattcga
      421 gcaagcgccg ggcgcgcccc gtttcctcgc gacggcgcag cgctatctcc agcggatcca
      481 gtacggcaac cgcgcgccgc tgggcgacca cgagccggcg cccgccgatc cgtccgcgtg
      541 gctgttcgag gtcgtgttcg attacggcga gcacgacgcg acagcaccga cgcccgatga
      601 tgagctcagg ccgtggcagc tccgcgccga tccgccgtcg agctttcgcg ccgggttcga
      661 ggtccgcacc taccggctgt gtcgccgcgt cctcctgttc catcgcttcg ccgagctcgg
      721 cagcacgccc tgcctggtcc ggtccacgga tttcacgtac gacgagagcc ctgccctcac
      781 gtacctcgtg aaggtcgagc aggccggcta cgtccgccgc gcggacggct cctacacgcg
      841 cgcggcgctg ccaccgctcg agctcgacta cgcgcggctg accgagctcg acgatacgct
      901 gcagatcctc gatcgcgcga gcctcgacgg gatcccggag ggcatcggcg gcgggacgac
      961 gcagtgggtc gacctcgatg gcgagggcat ccccggcgtg ctgcgggccg acgagcgcgg
     1021 ctggtactac aaggcgaacc tcggcgacgg ccgcctcgcg ccacctgcct tgctccgctc
     1081 gctgccgatc ccggccgagc tgtcgggcgg gttccagcag ctcgtcgatc tccagggcga
     1141 cggccgcctc gagctcgtgc attactcgca gccgctcgcc ggctacttca cgcgcacggc
     1201 agacggcagc tgggagccgt tcaccgcgct gcgctcggtg ccgagcatcg actggggcga
     1261 cgccaacctg cgcaccctcg atctcgacgg cgacggcttc cccgacgtgc tgatcaccga
     1321 gcacgacgcg ttcgtgtggt accgctcgct cgccacggac ggcttcgaac cggcgcgccg
     1381 cgtcggccgg atcctggacg aggagaaggg cccggcgctg gtgttcgccg acggcaccga
     1441 gtcgatctac ctcgccgaca tgggcggcga cggcctggtc gacatcgtcc gcgtccgcaa
     1501 cggcgatgtg agctactggc ccaacctggg ctacggccag ttcggaccca aggtcacgat
     1561 ggatggcggt ccccagttcg atcgcggcga tcgcttcgat ccgaagcgca tccggttcgc
     1621 ggacgtcgat ggctccggca cgtcggacat cctctatctc ggccgcgatg gcgtcgcgat
     1681 ctacctcaat cgagccggca acagcctgtc cgagccccag gtgatccgct cgctccccgt
     1741 ggtcgacacg ctggccggca ccaccgtcac cgatctgcgc ggccgcggca ccgcgtgcct
     1801 ggtctggtca tctccggcgc ccgcacgtga gggccgcccg atcgtctacg tcgacctcat
     1861 gaacggcgtc aagccacacc tcctggtctc gaccaagaac aacctgggtg ccgagacccg
     1921 gatcgactat gcgtcgtcca cccagttcta cctggcggac aaggcggcag gcacgccctg
     1981 gatcacccgc ctgccgtttc cggtccacgt cgtcgagcga gtcacgacct acgaccggat
     2041 cagccgcaac cgcttcgtca cccgctacac ctaccaccac ggctacttcg acggcgtcga
     2101 gcgcgagttc cgcggcttcg gccgcgtcga tcagctcgac accgaggagc tcgccgcgct
     2161 cacggccagc ggcgacttcc ccgcggccga caacatcgac gccgcctcgt acgtgccgcc
     2221 ggtgctcacc cggacctggt tccacaccgg cgcgttcatc gaccgcgatc ggatctcgac
     2281 ccagctcgcg caggagtatc acgtcgagcc cggcctcgcg gacgagcagc gacgcgccat
     2341 gctgctcgat gacacggtcc tgcccgacgg cctgtcggcc gacgacgtgc gcgaggcgtg
     2401 ccgcgcgctc aagagctcga tcctgcgcag cgaagtctac gcgctcgacg gcaccgaggc
     2461 cgaaggtcgc ccgtacctcg cgtccgagca cagctacgcg atcgtcctcg tgcagccgcg
     2521 cggcaacaac aagtacgggg tgttcttcgt ccacccgcgc gaggcgatcg agttccacta
     2581 cgagcgcaag ctgtacgacg tcggtggcac caagctcgcc gatccacgcg tcacgcacgc
     2641 gctgacgctc gaggtcgatg acttcggcaa cgtgctgcgc tcggtcgcca tcggctacgg
     2701 gcggcgccac gacagccccg atcccgtcct gacggcggag gacaggcagc gacagcgcca
     2761 gctccagatc acctgcaacg agaacgcgta caccaacgcg atcgaccagg acaatgctta
     2821 ccgcacgccg cagccctgcg agagccgcac ctacgagatc accaagctgt cgccaagcgc
     2881 aagcctcccc gacgtgacga acctgttcgg gttcgacgag atgctgcagg cgatcgacgg
     2941 ccttgaggat ggcgcccacg acctcgcgta cgaggacctc gccggtgccg gggcgacgca
     3001 agcccatccc taccggcggc tggtcgagca catgcgcacg ctgtaccgcc gcgatgatct
     3061 gaccggacca ctcgcgtttc atcatctcga gtcgcgggcc ctgccgtacg agagctacaa
     3121 gctggccttg actcccgggc tcctcacgtc ggtctacggc gaccgtgtca ccgacgcgat
     3181 gctggccgga gacggcggct acgtgcacag ccagggcgac gcgaactggt ggattccgtc
     3241 ggagcgcgcc ttctactcgc cgggcaagga cgacaccgcc gcccaggagc tcgcgtacgc
     3301 gatacagcac ttcttcttgc cgtgccggtt ccgcgacccc ttcgggcaca ccacggcgct
     3361 cacctacgac tcatacgacc tcctcgccct gcagacacgt gatgcgctcg acaacctcgt
     3421 gacgatcggc gcgcgcgatg cggccggtgc gatcgtcgac aacggcaatg actatcgcgt
     3481 gctgcagccg gtgcggatca tggacccgaa ccgcaaccgc aaggccgtcg cgttcgacgc
     3541 gctcggcatg gtggtaggta ccgcagtcat gggcaagcca gatgagcacg ttggcgactc
     3601 gctcgatgga ttcgtggccg atttgcccgg gagcgccatc gctgcgcacc tggccgatcc
     3661 gctcgccgat ccgcacgcca ttctccagcg cgccacgacg cggctggtct acgacctgct
     3721 cgcgtaccag cgaacccagg ccgaccttcg gccacaagcc ccggtcgtgt acacgctcgc
     3781 gcgggagacg cacgacgcca atctcgcgcc cggtgagaag accaaggtcc agcacagctt
     3841 ctcgtactcg gacggcttcg gccgcgagat ccagaagaag atgcaggcgg agccagggcc
     3901 actggttcca ggcggaccca tcctcggccc gcgctgggtg agcagtggct ggacgatctt
     3961 caacaacaag ggcaagccgg tccggcagta cgagccattc ttcacggaca cgcacgggtt
     4021 cgagagttcc aagatccacg gcgtcagctc ggtgctgttc tacgatccgg ccgaacgagt
     4081 cgtggccacc ctgcaccccg accacactta cgagaaggtc gtgttcgatc cgtggcagca
     4141 ggcgagctgg gacgtgaacg acaccgtcct gcagacagat cccgtggcag atccagacat
     4201 aggcggcttc atcgctcgcc tgcctgccga ggaatacctg ccgacgtggt acgagcggcg
     4261 gagcggcgga gcgctgggta ccgaggagcg agacgccgcg gacaaggccg ccgcgcacgc
     4321 cgatacgccg gcggcggcgc acttcgacgc ggtgggacgg caatttctca ccgtggcgca
     4381 taaccgtttc aaacgcgccg acatcgtcat cgaagaaaag tatcggacaa tgtcgatcct
     4441 cgacatcgag gggaaccaac gtgcagtcat cgacgcgaag gaccgcgtgg tgatgcgcta
     4501 cgactacgat ctactgagca accgcattca ccaggccagc atggaagcgg gcgagcgctg
     4561 gatgctcggc gatgttctgg gcaaaccgat ccgtgtctgg gacagccgcg gctttctacg
     4621 tcgcatggcc tatgacgagc ttcaccgacc cacgggattg tacgtgacgg agaatggcgg
     4681 ggagcggctc gccgagcgca cagtctacgg cgaggcccag ggcgacacgg ccaatcaccg
     4741 cggccaagtc taccagcgct tcgatgccgc cgggatcatg acgagcaccg gttatgactt
     4801 caagggcaac ctgggggaaa cccggcgcga tctattgccc acctacaagg tcgcggtgaa
     4861 ctggctgcag aatcctgcgg ccactgacgg cagtttcgcg accagcacga tgttcgacgc
     4921 cctgaatcgc ccgctcgctg ttacttcccc ggacggcagc atctaccagc cggtcttcaa
     4981 cgaagccagc ctgctgacaa atgtgaatgt gcgactgcgc ggtgcattca cggccacgat
     5041 cttcgtaacc aacatcaact acaacgccaa ggggcagcgt gagcggattg actacggcaa
     5101 cggcgtgagc agcacctatg agtacgatct gttccgctct cgcttggtcc atctcgtcac
     5161 gacgcgtccg accagcccgg atgctacggc ttcgcagctg ttccaagatt cgtcgctagt
     5221 acaggatctg cactactcgt acgactcagc tggcgacatc acgcgtattg aggatgccgc
     5281 tctcaagaca acctttcacg atggccaaga agtcaagccg gtagggacat acacctacga
     5341 tgccgtttat cggctcatcg aagccacagg gcgtgagcac attagccaga cagcgttcga
     5401 cttcaatcca tcggacggta attaccgtga ttatccattc gctggaagcc gagcacatct
     5461 caatgatcta caggcgttgt gcaactacat cgagctgtac gagtatgatc ctgtcggcaa
     5521 tttcgagacc ttggctcatc gtgctaacgg cgctggttgg acgcgccgct acgattatca
     5581 ggacaatagc ctccttgagg cgaacaagat gagcaatcgt ctgacgagaa ccatggtagg
     5641 ggatgggctg gatcgcgcag agtcttacac gtacgacgct catggaaaca tgacgtcgat
     5701 gccgcacctg gcagcgatgg cctggaactt caaagacgag ctgcaacgcg cggatctcgg
     5761 gggcgggggt gctgcgtact acgtctacga cggcaccggg cagcgtgtcc gaagggtcat
     5821 cgaatcgcag aatggcatgc ggcagaagga acgactgtat ttcggtggat tcgagatcta
     5881 ccgcgagtac agcggcgacg ggatcacgat taacctcgaa cgcgaatcgt tgcatgtcat
     5941 ggatggcaaa cagcgaatcg cgctgaccga agcgcagacg attcaagaac gaaatctagt
     6001 aaaggcgcca gatcctgtac tgcgttacca gctcggcaat cacttaggat ctgcgagtgt
     6061 ggagctggat agcaatggat ccttaatatc ctatgaagag tatagtccat atggaactac
     6121 atctttgcaa gcaggtcgca gtattgctga gattggtctg aagcgctacc gttacacagg
     6181 caaggagcgt gacaacgaaa caggtcttta ttacaacgaa gcaaggtact tcgcctgttg
     6241 gctggttcgt tggacgaccg tggacccttc cggcccagtt gatggactca atctatatga
     6301 gtactgtcgt agcaatccaa taatttttca tgatcctgat ggtcatgcga tagaaggcta
     6361 ttatattgcg cgcggccgat cagggaatga tcttccaagt cttttcttgg gtatttcagc
     6421 gcatgtactg attgcaaagg attacgaaag gaaacacggt aacgatcttt ctttctataa
     6481 ttacaaaacg ctcaggacca ttctcgagat ttctcgtgct ggggatccta aaaatctgaa
     6541 agtcgaggat cagcatctta agcctgatat cacgaatacc acgattcgcg aggtgttcga
     6601 ggccaagcca tggaacaaac aaggtatcaa aaagggctgg aaggaagtca atcggtatct
     6661 tgcgggctta aatgacgctg ccccgaagaa cgcgcagttc aaactcggca ccggccacag
     6721 cggtgaaata ctcgtgcgct tcgcgaatgg agtccccggc tggaagctat cctggcaaac
     6781 gacggttccg ggagtgatcc aataccgttg gcagacacta aatcgtaagc gacgtagcga
     6841 ggaatttcag cgtgagacct atcagcaagc gcaatgggtg gctctctcta gagccgaggc
     6901 tgctcagtac agcgagcagc tttatcgagg aatcgagaag catatcgaaa atgaggagaa
     6961 acggccagtc actcgtctcc cgttcttcat gacgctgggc atccacctgc tattgatgaa
     7021 ctcggttact cgtcgtcttt taggagggtc tcgaactcgg tctgaagaga gcgcgctcgc
     7081 aacgcctccg gcggctgatt ctcaacaggg gttgcagcca ccgagttatc ttcaacaagg
     7141 ccaacgaccg tcacaacgac cgctaccaag tggaccaatc tgtccggtcc catctcaatg
     7201 actcttcctc gtctaagtgg gcgctgcaat ggctgatcag tgatagagga acatgtttag
     7261 attgaaggat catttcatcg aagaggaggg gatgatgtat gtcaatcaca ttggataccg
     7321 accgattttc attggctaac ctgaaacaat tgatcgaaat gaatccatca atcgactggg
     7381 gtgcagtcgt gctggacgat ccaggcaggc gcgctgcgat cgattggcgt gaactcgatc
     7441 cggaaaccat ggtcccgctg gtcaaggcgt accagcgcct gatgcacatc ctgccgccgg
     7501 gcgaggagcg acgcgcgttg ccgctgctgg agagcggatt gcactcggcg atccagattg
     7561 cgagcatccc ccgggacgag ttcctgcgcc gctggggcgc gctgttcccc ggggacgagg
     7621 cgctcgggct cgccgtgcac cgcgccgcgc tcggccgtcg cagcgagctc gtcctccatc
     7681 acatccacga agtccagcgc aacgagccgc attaccgcgc cgcgcggttc aagtgagcag
     7741 ccatgagcaa tgaattgaag agtagcttca cgagcctgtt cgggccgatc gatcacgccg
     7801 aaggcaacga gtcgcgctcc gtgctgtccc cggcggcgta cctggtggac ctgctggagc
     7861 tgcgggacag catcgtcacc ggctggaccg actaccacgc gcgccgcccc gatgtccgag
     7921 gcattcacct ggaccaggcc agcacgttca ccgagatccc gttcctcgac gtcgccaacg
     7981 ccgtgatggg cgcggcgacc aggcagctgg tcggaggcac cgaggacgtc gataccgtcg
     8041 tcggtcgcgt gctcaccggc accctgttcc caccgccgct gccgttctcg gagcagcacc
     8101 tccgcctcca gctctacgcc gggaagctgg ggaccagcct ggacgagatc cagcggctgt
     8161 atcgcgccag cgccgatgcg cacctgtcgg cccggctccg cctggggctc ggtccggagg
     8221 agtacgcgct gttctccacc gcgcacgact cggacgacgg gatcaagaag ctgtgggggg
     8281 tcagcgatct cgccgcactg gacggctcgg acgtcggggg gatcgagcag aagctggggc
     8341 tctcgcgcaa ggagctcaag cagctcgtcc ggcaggatct gagcgcggcc gagctgtccg
     8401 agcccaggga cccgagcaag ccgaagcgga acgccgccga gtcgttcttc atcaaccagg
     8461 ggcccagcta cctcgtcctc gacgaggcgc cgctgaagct gcacctcaag gatggctcga
     8521 agctgtcgaa ccagcacctg gaccggatga tgcggttcgt ccgcctggcg cgtcgcctgg
     8581 atctcgagct ggccgacctg gactggctgc tccagaccgc gtgcggcaac gtactcgacg
     8641 gcgatgcact gcaggccatc gcgatcgcgc tcgatctccg caaggccacc ggcctgccga
     8701 tcgacgagat ctgcagcctg tggagtgtgg cgaaggacca tggtcagggc gacgggccga
     8761 tcccggccga tctgttcgac cgggcctaca acaacgacgt tccgatcacc ctggaggcgc
     8821 tggtcgccgg ccttgccgcg ggcgccacgg tcgatgcgct ggacgaccga ctgcaggcca
     8881 cgctgcgcgt gtcgggcgcc gacttcgcgt tcctcaaggc cgcgctgctc gcccgggggg
     8941 tggccaggcc gacgctcgcg taccccgcga tcgtcgcgtg gttctcggcg tttcgccgga
     9001 tcgcgacgct ggccacgatg ctggcgctga gcgtccggga ggtggtgatg ctcctcgatg
     9061 tgctcggggc gcagtggacg gtgtccgagc acgaggacct gtcgatcgcg atgccgttct
     9121 cggccgcggc gctgcccacg acgccgctcg gcctgctggt ccagcccgac gcaccgccgc
     9181 ggcggacgat ggacgtggtg cagaagctgt tccgggtcaa ggcgtggctg gatctgcgcc
     9241 agctctcggc gcgccagctg gcgtacctct gcctggagga tcacctcgac gcgcgccagc
     9301 ggctcgacga cggcccgccg atcgacgacg tccagcccga cgatgcggtc gaggccgcgc
     9361 tgaccgacct gcagcagtcg ctgctcgacg cgatggtggt gccgagcgcg ctgcagaccg
     9421 gctcgctgac cgccgacggc gcacgcgcgg tgttcgacgc gctccgcgac gcgcgggtgc
     9481 tggtgacgtt cgacgacccg gcccgcgggc tgctccgcgt ccagccgtcg gacggcgagc
     9541 tcgcggcggc gatgcagagc gggatccacg agcggctgga ggtccacgcg agcgacttcg
     9601 ccgcgctcgg gctcaccgac gccgatccgc tgttctcgct gctggtggcg cacgggtacc
     9661 tcgatcgcag ggaggaaacc gtcgagggag agctcgaggt ccaccacgtc atcgcgcccg
     9721 ggctggacgg ctacttcgcg tcgcccggcg gcgcggccac gttcaccttg ccgagcttcc
     9781 aggcgcacgc cgaggcgatc ttctcggtgc tggcccagcg cgtggatgcc gcgcgggtgg
     9841 acgccgccgc gctcgccgcc accgggatcg acggcgcaga cgtgccggcg ctgttcgccg
     9901 tgctccaggc gcacggctac gccgagcccg tccccggctc gactccgatc gactaccgcg
     9961 tcctcgtcga tgcaaagccg ttcttcagcg accccgcgaa cctggcgaag ttcgcgctcc
    10021 ccgacttcac gacggccttt gccgtgctcg ccgccaaggt ggccgcgttc cagcgcggga
    10081 tggccaacct cccgaccgaa gcgcaggagc tcggagggcg gctgtctgcg tcctccgatc
    10141 agcagatccg cgcgtggctg cgcagcctgt cggggctgat cgggctggcc gaggacgtga
    10201 ccgcgctggc gttcgcgtgg gcgttcggca cgccggacga gacgatgacg cagacgctgg
    10261 ccgcgctggc cctgccgctg ttccgcgcca ggcagcagtc gcagccggcg ctgggcgacg
    10321 cctacctcgc gtcgcgctgc cgccggctcc agcagctcgc gctgctgctc cgcaagacgg
    10381 caatgacggc ggaccaggcg cgcgtgtatc tcaccaacca gcaggttcac cggcggctgc
    10441 ccgagaccct gaacctgccc gatgggctcc tgtcctccgg aaagatcgat gcgctcgcca
    10501 cactgcccgg cggtgacctt gtcgtcatca gcgggccgac gtacgcgcag ttctcgcagc
    10561 acgacgatca cctggtcggc accggcacgc tggaccagat cccgggcgtc gcgctgcccg
    10621 atgcgtttcg cgcgcgcgtg cgggccagtg gcatcgacgc ggcgttcaac gacaacggcg
    10681 cactccagct gtgcgccggc gaccagtacg tgacggtctc cgcggcgggc accagcgcgg
    10741 ccaagccgat ctcggactgg ggccaggtcc gcaacaacct ccagcagcat gcgcgggtcg
    10801 acgcggcggt gcaggccaag gacggccggc tgttcctgtt cagcggtgac cagtacgtcc
    10861 tctacagcgc gccgcagcag ctgctcgcgg gccccgcgtt cgtggacgag cgctacccga
    10921 gatcgatccg cggcaagttc gagaacgagg gcgtctcgcc gctgcccgcg ctgatgttct
    10981 cgggggtcga cgccgcgttc cgcgacaccg acgacaccta ctacttcttc gcgggcaacc
    11041 ggttcagcca ctcgaccgat ccgttcaacg tcgtcaagat ccggccggtc tggggccacg
    11101 tcctcaacga cctgttcgag gacaaccggg tcgacgcggc gttcgtgctc ggcggcgtca
    11161 cctacctcac gcgcaggaac cagctcctcc gctacaccgg ctcggcctac cagatcgtcg
    11221 acgagggatt cccgatctcg ttcggcaacg tcgccgacag cgagccgctg ctgcgcgtgc
    11281 tccggcggtt ccccaacggg ctcgacgccg cgctggccgg cagcgatggc aagctctacg
    11341 cgttcaagga cggcgcctac gccagctcgg ccgcccccga cacctcgctg acgatccgcg
    11401 accactgggg ccgcgtccgc aacgtgttcg tggacaacca gcgcgtggac gccgcgctgg
    11461 cctacggcgg cgcggtctac ctgttctcgg gcgaccagta cgtccgctac tccggcgcga
    11521 gctaccagct cgtggacgag ggctaccccc ggcgggtccg gcccagctgg aacgcgctcg
    11581 agaagatcgg cgtggtcccc gacgggctgc cgctgccgat caccgcggtg gcggtgggcc
    11641 gcgcgcccgg cggcacctcg gacgacgtct acttcttcgc cggcaaccag ttcgccggtc
    11701 ccaccggcgc actcgacgac atcaaggcgc ggtgggcgcg cgtccgcaac aacctcggga
    11761 aggtcggcgt ggtcgacgcg gcgatgctcg acggcagcgg gcggatgtac ctgttctcgg
    11821 gcgatcagta ctaccgctac agctctcccg atcagacgtt cgtcgacgag acctatcccc
    11881 ggcggctcgc cggcaactgg gtgcaggagg gcagcggcta cgcgctgccg gatgcgttcg
    11941 ccggcgggat ctcggcggcg ctgcgcacca ccgatggcac cctctacttc ttcagcggcc
    12001 agagctacgc gcgcgtcgac accgcgccgg ccacgccgcg cgcgaacaac caggactggg
    12061 gcgtggtccg caacgtgatc caggcgcaga acgcggtggg cgcggcctac gtcgatcccg
    12121 ccgggaagac gtacgtgctc ggcggcgatc aggtcgtccg ctacacgggc accgcgtacg
    12181 acgtcgtcga cgagggcttt cccctggcga tcgccacgcg gtggccgagc ctcccggcga
    12241 gcttccgcag cgacatcgac gcggcgctgg tgttcaagtc gccggtcgac aacgtgcagc
    12301 ggctgtacct gttcaagggc gacagctact gccggttcag cacgggggac ttcacccagc
    12361 tcgatgccgg ataccccagg cgctatctgg acgcgatcca ggccgagggc ctgtggttcc
    12421 gggggctcgc cgtgcacgag ccgaacccga actcgtccca ccaggacgat ccgatcgcct
    12481 cggtggaggc gatctatgtc gacacgttcg gcggtcagcc ccggatcaac gtgttctacc
    12541 gccacgacgg cggcatccag tggcggcgga tcttccagag cgggaactgg ggggcgccgg
    12601 tccgcgtcga gaccctctcc gactacctgc cgttcacccg gctggacgcg gcgttggtcg
    12661 cgaccgacgg cacgctgcac gcgttctccg ggaaccagta cgcgtcgcgc ccgccggcgg
    12721 gcggtccgct caccgcgccg gtgccgatca acagccgctg ggcgcgggtg ttcaaccagt
    12781 tcgcggacct cggccgggtc gacgcctgcc tgacgatggg cgacgggcgc acctacctgt
    12841 tctgcgccag gcagttcatc aagtacaccg gcacgctgcg gccgggcgcc gccgacttct
    12901 tcgtcgacga gggctacccg ctgcgcatcg acccgaactg gcccgcccag ggcgtccccg
    12961 tgacgctggc gcccgagctc caggcggcgg ggtacgacct gtgccgcgac gccaccggca
    13021 aggtgcattt cttcaacgga acacgctacg cggtctcggg caccgcgggc gacgtgctgt
    13081 tgacctcgcg gtggggcaag gtggagaacc ggttccagga cctcgaccgg gtcgacgcgg
    13141 cgtaccgcgc cgagaacaac aagctgtacc tgttctgcga cacgcagtac acgcgctaca
    13201 gcggtgcgct gcagccggga tctccggact tctacgccga cgagggatac ccgcggcacg
    13261 tcaccaccgg ctgggcgtcg gagggtctgc cgatcgccct gcccgcggtg tggaacgcgc
    13321 tcggctcggc gatcttccgc gacgcgcagc agacctacgt gttctccgga tcgtcgttca
    13381 ccagctcgca gtcgccggcg cccgcgcccg tgatcccgca gtgggcgaag gtccgcaacc
    13441 agatccagtc gcagaaccgc gtcgacgccg ggttcgtgct cggccagggc gccggcgcgg
    13501 tgacgctggt gttctgcgac gaccagtacg tccgctacag cggggcctac gacggcttcg
    13561 tcgacgaggg ctaccccaag gtcatcgccc acctggcggc ggccgacggc gcgttccccg
    13621 ggctgcccga cccgctgccg tccgggctcc gcggcttctt cgccgggacc gacggcaacc
    13681 tccacgcgtt cgggccgcag ccgtccgatg cgtcgcagcc ccagctctac gtgtcgtccg
    13741 ccggccccgc caccctgcgc ccgctcgacc agcgctgggg catcgtcgac aacaagctgt
    13801 gggacaacga gttcgtcaac gccgcgctgg tcgccggcgg caagctgttc ctgttcagcg
    13861 gcgacca
//
LOCUS       DASVOR010001300         2385 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_510062,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001300 DASVOR010000000
VERSION     DASVOR010001300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510062"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VF516_24805"
     CDS             <1..500
                     /locus_tag="VF516_24805"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8110986.1"
                     /translation="AALLRARQRASTRPVQRSITVDAPAEVLHARWRDPDLLTQIMQP
                     LGTVTADPSGGGALRWRVVGPFGRTIEWVTRIVEDQPPRLIRWVTVDGAPIESGGSVE
                     FRQDRPELGTTVTLRLRLGIAIPHLVPKMGIAKILRRFKSLVETGEVPTLEHNPSARA
                     SALAY"
     gene            511..1680
                     /locus_tag="VF516_24810"
     CDS             511..1680
                     /locus_tag="VF516_24810"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX8110987.1"
                     /translation="MRALCWNGVNALSVETVPDPEIVAPQDAIVRVVLTTTCGSDLHF
                     IDGYIPTMRKGDVIGHEFMGEVVEVGRDVKRIRKGDRVVVPSFICCGRCWYCQHELWS
                     LCDNTNPNAELQELALGYPTAGIYGYTHAFGGYAGSHAQYVRVPHADVDCFVIPEGVR
                     DDQALFLSDAAPTGYMGADFCNIKPGDTVAVWGCGGVGLMAQRSAFLLGAERVIGIDR
                     FPERLRLARERVGAEIIDYTAVDSVTDTLREMTGGRGPDACIDAVGMEAHGIGLQYAY
                     DRVKQALRLETDRGEALRQAMYACRKGGTLSILGVYGVMDKFPVGVMMNKGLTVRTAQ
                     QHGQKYVPRLLEHVAKGELDPSFLATHRFSLEDGPRGYAMFKHKTDGCVRAVFAP"
BASE COUNT          378 a          791 c          833 g          383 t
ORIGIN      
        1 gtgcggcact gctgcgcgct cgccagcgtg ccagcacgcg gcccgtgcag cgctcgatca
       61 cggtcgatgc gccggccgag gtgctccacg cgcgctggcg ggatcccgac ctgctgacgc
      121 agatcatgca accgctcggc accgtcacgg cagatccgag cggtggaggt gcgctgcgct
      181 ggcgggtggt cggtccgttc ggccgcacca tcgaatgggt gacgaggatc gtcgaggatc
      241 agccgcccag gctgatccgc tgggtgaccg tcgatggtgc cccgatcgag agcggaggct
      301 cggtggaatt tcgtcaggac cggcccgagc tcggtacgac cgtgacgttg cgcctgcggc
      361 tcggcatcgc gattccgcat ctggtaccca agatggggat cgcgaagatc ctgcgcagat
      421 tcaagagcct cgtcgagacc ggcgaggtcc cgacgctcga acacaacccc tcggcgcgcg
      481 cgagcgcgct cgcctactag gagcttcatc atgcgcgcac tgtgctggaa cggggtgaat
      541 gccctgagcg tcgagaccgt cccggacccc gagatcgtgg ccccccagga tgcgatcgtg
      601 cgcgtcgtcc tgaccacgac gtgcggctcc gatcttcact tcatcgacgg gtacataccg
      661 acgatgcgca agggcgacgt gatcggccac gagttcatgg gcgaggtcgt cgaggtgggc
      721 cgcgacgtca agcgaatcag gaagggcgac cgcgtcgtcg tcccgtcgtt catctgctgc
      781 ggcaggtgct ggtactgcca gcacgagctg tggtcgctgt gcgacaacac gaacccgaac
      841 gccgagctcc aggaactcgc gctcggctac ccgaccgcgg gaatctacgg gtacacgcac
      901 gcgttcgggg gctacgcagg ctcgcacgcg cagtacgtcc gggtgcccca cgcggacgtc
      961 gactgcttcg tgattcccga aggtgtccgc gatgaccagg cgctgttcct ctcggatgcc
     1021 gcgccgaccg gttacatggg cgccgacttc tgcaacatca agccgggcga caccgtcgcg
     1081 gtgtgggggt gcggcggcgt cggtctgatg gcccagcgca gcgcattcct gctcggggcc
     1141 gagcgcgtga tcggcatcga tcgattcccc gagcggctgc gcctcgcgcg cgagcgcgtc
     1201 ggggcggaga tcatcgacta cacggccgtc gacagcgtca ccgataccct gagggagatg
     1261 accggcggcc gcgggcccga tgcctgtatc gacgcggtcg gcatggaagc ccatggcatc
     1321 ggcctgcagt acgcctatga tcgcgtcaag caagcgctcc gtctggagac cgatcgcggc
     1381 gaggcgctgc gtcaggcgat gtacgcctgc cggaaaggcg ggacgctatc gatcctcggg
     1441 gtctacggcg tgatggacaa gttccccgtc ggcgtgatga tgaacaaggg cctcaccgtt
     1501 cgcacggcgc agcagcacgg gcagaagtac gtgcctcggc tgctcgagca cgtcgcgaag
     1561 ggcgagctcg atccgtcgtt tctcgccacc caccggttct cgctcgagga cggaccgcgc
     1621 gggtacgcca tgttcaagca caagacggac ggttgtgtcc gcgcggtgtt tgctccgtga
     1681 ttcgcagggc gtgcggtcgg gtgccccgcg cggatgctcc cagcacacgt tgaatgcgac
     1741 cgcgcccatg gctgcagcag tcgacatctc gctcacgtgc tccaagaaga acgactctcg
     1801 cgcttgcggc tgacacgttc agcgcagcga gaaacgccgc catgtgctcc acgctcgcag
     1861 tggtgcggct gtgtcgatcg agtagacctg cagaccgcgc gcccaccgca cgcggtgggg
     1921 cgggccgacg cgtccgacgt gacagcgcag gccgcttgcg tgcgcgagct ggacccacgt
     1981 tgcggtggtc gcgagcttcc acgacagcgt gccacagtcg gtccggcggg ccctcaacga
     2041 cctgggcatt agccacgacc tcgtcgatac ggtccgggtg aaccgggcct gctggaactc
     2101 gtcgcgcggt acgggcagac gcgtgcagca ccgtgcgctc gggttcgcgg cgtcggtagc
     2161 cggtgagcgg ctgtccggcc gcgccggacg ttcgctcgta caggccggcg cgcgagcaac
     2221 tgcccgcgtc ttgaaacatc cgcgccaagc tgtgctggct acctcgtcgc ctacttaggt
     2281 tatgtcgtgt ccgactgccg gacgatggcc acggccggag cccaccctgc gggagtcggc
     2341 atagcagcgc agatcgcagc cggacgggat cggggcgccg ggttg
//
LOCUS       DASVOR010001301         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_305731,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001301 DASVOR010000000
VERSION     DASVOR010001301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305731"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VF516_24815"
     CDS             complement(<1..181)
                     /locus_tag="VF516_24815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110988.1"
                     /translation="MTDGPAVQRRSAAPARASEPDAVKAAAARGTAGTAGTLPHLERI
                     QRLFGRHDVSHVRAHV"
     gene            complement(620..691)
                     /locus_tag="VF516_24820"
     tRNA            complement(620..691)
                     /locus_tag="VF516_24820"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(657..659),aa:Met,seq:cat)
     gene            1332..1405
                     /locus_tag="VF516_24825"
     tRNA            1332..1405
                     /locus_tag="VF516_24825"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1364..1366,aa:Gln,seq:ttg)
     gene            1435..>1683
                     /locus_tag="VF516_24830"
     CDS             1435..>1683
                     /locus_tag="VF516_24830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110989.1"
                     /translation="MKLDKSTSLFFATKIDSKLREGLALSKPGDKKYFDGSSEEFLRV
                     MEIGDEKDKERWIGKIIKPGPAVTEVDDIQRNVLSILRR"
BASE COUNT          281 a          545 c          597 g          260 t
ORIGIN      
        1 cgacgtgcgc gcgcacgtga ctgacatcgt ggcggccgaa cagccgctgg atccgctcga
       61 ggtgaggcag cgtccccgcg gtgccggccg tgccacgcgc agcggcggcc ttcaccgcgt
      121 cgggctcgct ggcccgtgcc ggggctgcgc tgcgccgctg caccgccggg ccgtcggtca
      181 cggggcctgc gccgatcgcc tcgacaagcg tgcgcttgcc gaccgcgctg agggtggcac
      241 cggctgtgcc gggtactgca cgggggctcg agcctcgagc ggcaccggct tcgtgagtca
      301 tcattgcgac tgtaggcagt gatcgcggat accaccatta cgaccgatta ccgccgcatg
      361 cccagcctcc cgccgccgga gttcggcaca tgacgacagc acggtagttc tcgccgttgt
      421 ttgcgacgtg gccggtggtc gcgcgctcgg acccgcacag cgccatcctc cgcatcgacg
      481 tgccggcgcg ctcgcgccga cgaacgcgca cgactcgatc gaccatgtcg tcgaaggccg
      541 gcagcgcgcc cgagtgcacc gcgtcgttga ctcgtggttc ctggctgtag cctggacctg
      601 ctcgctgtcg aaaaacttgt ggcggagggc agatttgaac tgccgaccaa gggcttatga
      661 gacccctgct ctacccctga gctaccccgc caagcggcga aaacctaaag gcaaggagac
      721 cttcaggcaa gcggttcgag acggttctcg accgccgccg gtcgatctga gggtagcggc
      781 cgaggggtgg ggcggcggag cgcatgcgat cacgacagga tctcggcggc gggggagcgg
      841 ggcagcgcgc cgggtgcacc cgggcgtgag tcgatctaac gggtcgatct aacgcaaatt
      901 ctgggttgac tcatgtcggg cgaagcccca ggtgcccgag gaacgcgggt cccggcggcg
      961 ggcgcgcgac ggtcgggtgg cgatcacccg tgccgcgcgg gctcccgatc aacgcgccgg
     1021 tcgagatcgc gctcaccgcg ctcgaccgcg catcgccgcg cgcgacggcc gtcgggtggt
     1081 gcgcacgccg aactgcaagc cgaggtcacc accggcgaca tccgccaggc gcagctcgat
     1141 cgccacgacg cagagcgggt cgcctgctgg acgtgatcgc ggcggggccg tgccggcgcg
     1201 cgcggggtat cagatcggcg ggcgctcggc tggtgagccg gctacggatc cggtaacctc
     1261 agctatcgct taacgaaatc aagaacatcg ctggccgttg gccgaaggct agggtatcgt
     1321 cggcgtcgca ctcggggatc gtctaatggt aggacagcag cctttggagc tgctcatcaa
     1381 ggttcgaatc cttgtccccg aaccaggcga tcagtctgcg gatggatgca cgccatgaag
     1441 ctcgacaaga gtacctctct gttctttgcg acgaagatcg acagcaagct ccgcgagggg
     1501 ctcgcgctgt cgaagcccgg ggacaagaaa tacttcgatg ggagctccga ggagttcctt
     1561 cgcgtgatgg agatcgggga cgagaaggac aaggagcgct ggatcggcaa gatcatcaag
     1621 ccgggcccgg cggtgaccga ggtcgatgac atccagcgca acgtgctgtc gatcctgcgg
     1681 cgg
//
LOCUS       DASVOR010001302         1695 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_510091,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001302 DASVOR010000000
VERSION     DASVOR010001302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1695
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510091"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VF516_24835"
     CDS             complement(<1..694)
                     /locus_tag="VF516_24835"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8110990.1"
                     /translation="MTTVAALALIIALIVAAVVAALVAVRRWRKPTAAPERVGIAEAP
                     LEGVVPVPGPIERGPPLPVVLVHGLFGFDRIGVPGVRFDYFRGIARHLETLGCHAHAV
                     RLPAAGSVPERARELVAAIDALPHPRIDLIAHSLGGLDARYALTHLGLARRVRSLVTV
                     GTPHHGTPLADLALRGPLDWARRIIGALGVPLEALEWLGTDALARFNAQVVDVPGVRY
                     ACVVGGMHREDSI"
     gene            1082..1639
                     /locus_tag="VF516_24840"
     CDS             1082..1639
                     /locus_tag="VF516_24840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX8110991.1"
                     /translation="MALVAYGVTPEAIRIGYQPDQPVPYSHALHAGELGLDCRYCHST
                     VERAGKAAVPPAATCMNCHSTVKKDSDLLLPIRQAFAEDEAVHWTRVHDLPDYVYFNH
                     SAHVNAAVGCESCHGRIDQMVKVYQAKPLTMEWCLGCHRNPTPFLRNPENVTKMGYQP
                     QPGEGEDVRKKNGVNPPTNCSTCHR"
BASE COUNT          290 a          588 c          576 g          241 t
ORIGIN      
        1 cgatgctgtc ctcgcggtgc atgccgccga ccacgcaggc atagcgcacg ccggggacgt
       61 cgaccacctg ggcgttgaac cgggcgagcg cgtcggtgcc gagccactcc agcgcctcga
      121 gcggcacgcc gagcgcgccg atgatccggc gcgcccagtc gagcgggccg cgcagcgcga
      181 gatcggcgag cggcgtgccg tggtggggcg tgccgacggt gaccagcgat cggacgcggc
      241 gcgccagccc caggtgggtc agggcgtagc gcgcatcgag gccgcccagg ctgtgcgcga
      301 tcaggtcgat gcgcggatgg ggcagtgcgt cgatcgcggc gaccagctcc cgggcgcgct
      361 ccggaaccga gccggcggcg ggcaggcgga ccgcgtgtgc gtgacagccc agggtctcca
      421 ggtggcgcgc gatgccgcga aagtagtcga accggactcc cgggacgccg atccggtcga
      481 acccgaaaag tccgtgtacc aggacgaccg gcagcggtgg gccgcgctcg atcgggcccg
      541 ggaccgggac aacaccctcc agcggcgcct ccgcaattcc gactcgttcc ggtgcggcgg
      601 ttggtttgcg ccaccgccgt actgccacca gcgcggccac caccgcggcg acgatcagcg
      661 cgatgatcag ggcgagcgcg gcgaccgtcg tcacggccga ggcagcgtgc cacgtctcac
      721 gcagcgagcg aacgtcatgt gcgtctcatg ctcgcgccac gctgcaattc ctgcgtcatt
      781 ctgtcgcgga gatggtcagt gggtgtgaat tcgacgccgc ggccctctcg acggaagcat
      841 cgagtgccgg cgcagcgcga ggcatctcga tctgcgctgc gaccagacgc cgctccccga
      901 actcccgcca caggcttgcg agatgtgctc tgcctgtggt gcaaagctct ctctcgcaca
      961 cgggcaccgt atcgtcaggc ccaacgcagg aaccttcatg ccgcgtttca tattccccga
     1021 atggaccgag acgctcaaac ggtggatcaa cctgttggtc ctcggtgcgc cggtatacct
     1081 ggtggctctg gtcgcatacg gcgtgacccc ggaggcgatc cggatcggct atcagccgga
     1141 tcagccggtg ccgtacagcc acgccctgca cgccggtgag ctcgggctgg actgccggta
     1201 ctgccactcg accgtcgagc gcgccggcaa ggccgcggtg ccgccggcgg cgacgtgcat
     1261 gaactgtcac tccaccgtca agaaggactc ggacctgttg ctgccgatcc gccaggcgtt
     1321 cgccgaggac gaagcggtgc actggacgcg ggttcacgac ctgccggact acgtctactt
     1381 caaccacagc gcacacgtga acgcggcggt cggctgcgag tcgtgtcacg gccggatcga
     1441 ccagatggtc aaggtctacc aggccaagcc gctgaccatg gagtggtgcc tgggctgcca
     1501 ccgcaacccg acgccgtttc tccgcaaccc ggagaacgtc accaagatgg gctatcagcc
     1561 ccaaccgggc gagggcgagg acgtccgcaa gaagaacggt gtcaacccgc ccaccaactg
     1621 ctcgacctgc catcgctagg acccgacgat gtcccaagac gaagagcttc acgccatgga
     1681 tgcagatcga tccga
//
LOCUS       DASVOR010001303         5071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_14183,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001303 DASVOR010000000
VERSION     DASVOR010001303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14183"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF516_24845"
     CDS             <1..442
                     /locus_tag="VF516_24845"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8110992.1"
                     /translation="AEATRAAVAAGSDAFLLHVATHASQANGEAAFVLYDGVLPANEI
                     VAQRIAPRLAVIATCRSQVDDDPAGSLVAAFLAAGAPGIIGVKRSLDDRDGAALMADL
                     YRLQGAEDPVRALALAQRAAIAANRPPSAWATVSFFGVGGWVWH"
     gene            494..865
                     /locus_tag="VF516_24850"
     CDS             494..865
                     /locus_tag="VF516_24850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110993.1"
                     /translation="MSGAGCKDKSENAPELRSGMAKEAIIGTPPGIGARVKVVRRPNG
                     DLGVISPDGKQVIVVQPDGYQVCEPSEDALLCGPLTQMAVGCPGCSADPCPCDNPVCR
                     PGCKQTILKPMGSGSANPGTP"
     gene            940..1524
                     /locus_tag="VF516_24855"
     CDS             940..1524
                     /locus_tag="VF516_24855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8110994.1"
                     /translation="MDEVDAALSRGDRQHAFTLLMTRYGDAVYRYALATTGDAPLAEE
                     VRQQVFVEVYRDLPGFGRRAPVRGWLFAIVRHRCLDATKAFRRWGLRYKNELPSDPES
                     GLIAGLVIDQSELMEQLDRHRLARVVERCLSTLAPAAREAVLLRYQQELSYDEAAEVT
                     GERPGTLQQRVARALPVLRKCVEAQLRTGGPGGL"
     gene            1524..2210
                     /locus_tag="VF516_24860"
     CDS             1524..2210
                     /locus_tag="VF516_24860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110995.1"
                     /translation="MACERYWREGILLDERGEHDPHRETCDDCRRAHRERELLIRALP
                     EVGAASSGDPDWQNHVWRRIARAETSRARRSYWLGGALAAAGVLAAIGLYLTRDPYPD
                     DRIAGEVARSDDPRQPKIDIVSSQTPARSRSARVGDRVRISVRPGCEVRIYRADRLVL
                     RCPAWQTSPGCMPDAAGLVADAVLTTAGDYQLVTITSATVDPAGALDRDLAAVVDAGG
                     DYTLNELSVR"
     gene            complement(2256..2969)
                     /locus_tag="VF516_24865"
     CDS             complement(2256..2969)
                     /locus_tag="VF516_24865"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8110996.1"
                     /translation="METVSVDESLPALEPLRVTVIPLVSTPITQAVVAELRRGAMQVE
                     LQTAEVLDAAPEAPVYVVSVDPATGAPLADRFVAWAADSALRPGLIALIENGNARDAE
                     NLLAAGFDDVAVTPVSTREIAARVRAVHRRVHWKGVPNGRLRFGELTLDLYGRALWFR
                     GKTISLTSIELAVLRELIKAHGRPLSRIELLDRAWGEEDLEVSERAVDNVILRLRRKL
                     PEPDVIETVRSVGFRLASP"
     gene            3224..3832
                     /locus_tag="VF516_24870"
     CDS             3224..3832
                     /locus_tag="VF516_24870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110997.1"
                     /translation="MGQPSVNEYPRYRLEEGKPCIDVRIASLDHLFDNRDPAPFRERD
                     LDPALVEYLLGIGHDLTARDGFRIVFWLERTCQPGVVESAYRAHFAYALARLERVRRR
                     ARRAGQIALLLGAGLILALMSLSQLAVQAVPGSLGAALREGLVISCWVVMWRPVELLI
                     YDWIPVWEERRCMRRLLAAPIEVRAGHGPDVKPVVGPVAAVG"
     gene            3891..4793
                     /gene="rarD"
                     /locus_tag="VF516_24875"
     CDS             3891..4793
                     /gene="rarD"
                     /locus_tag="VF516_24875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEX8110998.1"
                     /translation="MSDSRRGLVHGIAAYFLWGIAPVFWKLLRDVSWVESLSHRVVWG
                     VIAFGVIAAAVGAGPEVREAARDRGTLAAMALSGVMLVANWGTFIWAVARGHILDASL
                     GYFIIPLISVGLGMLVLGERPRRLQWIAIAAAAVGVAIQTWYAGGLPWISIVVAVSFG
                     LYGLIRKVARVDSLVGSTLETALLVPVAASYLVILALRGGGQLGHAATGTQLLLLSTG
                     AVTAAPLLLFNSAARRLPLSTLGFLQYLGPTCQFILAIAVYGERFATEQLIAFGAIWF
                     GLAVFSVDLVRAARAAAPVSRTGR"
     gene            complement(4774..>5071)
                     /locus_tag="VF516_24880"
     CDS             complement(4774..>5071)
                     /locus_tag="VF516_24880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8110999.1"
                     /translation="VEPEVVLMDEPCSALDPIATARIEELMRELKSRFTIVIVTHNMQ
                     QAARVSDRTAFFTTELDSKNDRRTGTLVEYDATSKIFAAPSDERTENYITGRFG"
BASE COUNT          684 a         1672 c         1917 g          798 t
ORIGIN      
        1 ggccgaggcc acgcgcgcgg cggtcgccgc cggcagcgat gccttcctgc tccacgtcgc
       61 cacccacgcc agccaggcca acggcgaagc ggcgttcgtc ctgtacgacg gcgtgctgcc
      121 ggcgaacgag atcgtggcgc agcggatcgc gccccggctc gcggtgatcg cgacctgccg
      181 ctcgcaggtc gacgacgatc ccgccggctc gctggtcgcg gcgttcctcg ccgcgggagc
      241 gccggggatc atcggcgtca agcgctcgct cgacgaccgc gacggcgcag cgctgatggc
      301 ggatctgtat cgactccagg gagccgagga cccggtacgg gccctggctc tcgcgcaacg
      361 tgcggcgatc gccgcgaaca gaccacccag cgcgtgggcg accgtgtcgt tctttggagt
      421 cggcgggtgg gtctggcact gaaaggggct ggtgatgttt cgaccactgt tgatctgtgt
      481 gatctgtctc ggggtgagcg gggcggggtg caaggacaag tcggagaacg cgccggagct
      541 gcgcagcggc atggcgaagg aggcgatcat cgggacgccg ccggggatcg gggcccgcgt
      601 caaggtggtg cgccggccga acggcgacct cggcgtgatc tcgcccgacg ggaagcaggt
      661 gatcgtcgtc cagccagacg gctaccaggt ctgcgagccg tcggaggatg ccctgctgtg
      721 cgggccgctg acccagatgg cggtcggctg cccgggctgc tcggccgacc cgtgcccgtg
      781 cgacaacccg gtatgccggc cagggtgcaa gcaaaccatc ctcaagccga tggggtccgg
      841 gagcgcaaac cccggcacac cctgagattc ctgtagtatg agatccgggt gcgtcgcctg
      901 ggtgatgtgg gcagacggaa ggcacccgcg acgttcgaag tggacgaggt cgacgccgcg
      961 ctgtctcggg gggatcgcca gcacgcattc acgctgctga tgacgcggta cggcgatgcc
     1021 gtatatcgct atgccctggc gacgaccggc gacgccccgc tggcagagga agtgcgccag
     1081 caggtgtttg tcgaagtata tcgagatctt ccggggttcg ggaggcgtgc gccggtgcgc
     1141 ggctggctgt ttgccatcgt gcgccatcgc tgtctggacg cgaccaaggc atttcgccgc
     1201 tgggggctgc ggtacaagaa cgagctaccc agcgacccgg agagcgggct gatcgccggg
     1261 ctggtgatcg atcagtccga gctgatggag cagctcgatc gccatcgcct ggcgcgggtg
     1321 gtcgagcgct gtctgtccac actcgcgccg gcggcgcgag aagcggtgct gctgcgctac
     1381 cagcaggagc tgtcctacga cgaggcggcg gaggtgacgg gcgagcgacc cggaacgctg
     1441 cagcagcgcg tggcacgtgc gctgccggtg ctccggaaat gtgtggaggc gcaactgcgg
     1501 acgggaggtc cgggaggatt atgatggcat gcgaacgtta ttggcgggaa gggatcctgc
     1561 tggacgagcg aggcgagcac gacccgcatc gcgagacctg tgatgattgc cggcgcgcgc
     1621 atcgcgagcg cgagctgctg atccgggcgc tgccggaggt cggcgcggcg agctccggcg
     1681 atcccgactg gcagaaccac gtgtggcgac ggatcgctcg cgcggagacg tcgcgcgcgc
     1741 ggcgatcgta ctggctcggt ggcgcgctgg cggcggcggg cgtgctggct gcgatcggcc
     1801 tgtatctgac ccgcgacccg taccccgacg accggatcgc cggggaggtg gcgcgctcgg
     1861 acgatcccag gcagccaaag atcgacatcg tgtcgagcca gaccccggcg cggtcgcgct
     1921 cggcgcgggt tggcgatcgg gtccggatct cggtccgccc gggctgcgag gtgcggatct
     1981 accgcgccga tcgcctcgtg ctgcgctgtc cagcgtggca gacctcgccg ggctgcatgc
     2041 cggacgcggc aggtctggtc gcggacgcgg tgctcaccac cgccggcgac taccagctgg
     2101 tcacgatcac atcggcgacc gtcgatccgg ccggcgcgct ggaccgggat ctcgcggcgg
     2161 tggtggatgc cggcggcgac tacacgctca acgagctgtc ggtccgctga ggctgttgaa
     2221 aaacgatgcg aggagcgggg cccgcagcgc gttgttcagg gcgaggcgag ccggaagccg
     2281 acactgcgca cggtctcgat cacgtcgggc tcgggcagct tgcggcgcag ccgcaggatc
     2341 acgttgtcga ccgcgcgctc ggagacctcg aggtcctcct cgccccacgc ccggtcgagc
     2401 agctcgatcc gcgacagcgg gcggccgtgc gccttgatca gctcgcgcag caccgcgagc
     2461 tcgatcgagg tcagcgagat cgtcttgccg cggaaccaca gcgcgcggcc gtagaggtcc
     2521 agcgtcagct cgccgaaccg cagccggccg ttggggacgc ccttccagtg gacgcggcgg
     2581 tggaccgcgc gcacgcgcgc cgcgatctcg cgggtcgata ccggggtcac cgcgacgtcg
     2641 tcgaacccgg cggcgagcag gttctcggcg tcgcgcgcgt tgccgttctc gatcagcgcg
     2701 atcagcccgg ggcggagcgc gctgtcggcc gcccacgcga cgaatcgatc ggcgagcggc
     2761 gcgccggtcg ccgggtcgac gctgacgacg tagaccggcg cctcgggcgc ggcgtcgagc
     2821 acctcggcgg tctggagctc gacctgcatc gcgccgcggc gcagctccgc gacgaccgcc
     2881 tgggtgatcg gcgtcgagac caacgggatc acggtgacgc gcagcggctc cagcgctggc
     2941 aagctctcgt cgaccgacac ggtttccacg gccgacgcta cgcagcggga gtgaccagac
     3001 cggggcgatt ctgcaaccga acggtgacat gacgaggacg agacgcgatc gcggcgttgc
     3061 cgtcacgtag gcaatctggc gaacgagaat ttcgcgcctc gatccggcgt gccgatctcc
     3121 gctcgagggg cagaacaggt ggttctggct cgggttttct tggcagcctg ccgcatcgtg
     3181 cggcaggggc agcgagcgag gatctcgcat cgccggccgg gccatgggac aacccagcgt
     3241 gaacgagtat ccacgatatc gcctcgaaga gggcaagccg tgcatcgacg tccggatcgc
     3301 gagcctggac cacctgttcg ataaccgcga tccggcgcca ttccgggagc gcgacctcga
     3361 tcccgcgctc gtggagtacc tgctggggat cggccatgac ctgacggcgc gcgacgggtt
     3421 ccggatcgtg ttctggctcg agcgcacgtg tcagcccggc gtggtcgagt cggcgtatcg
     3481 cgcgcacttc gcgtatgcgc tcgcccggct ggagcgcgtg cggcggcggg cgcgccgcgc
     3541 ggggcagatc gccctgctgc tcggcgcggg cttgatcctc gcgctcatgt cgctgtcgca
     3601 gctcgcggtc caggcggtcc ccggatcgct cggcgcggcc ctgcgcgagg ggctcgtgat
     3661 ctcctgctgg gtggtgatgt ggcgtccggt cgagctcctg atctacgact ggatcccggt
     3721 gtgggaggag cgccggtgca tgcggcggct gctggccgcg ccgatcgagg tccgggccgg
     3781 ccacgggccg gatgtcaagc cggtcgtggg cccggtcgcg gcggtgggat aatcgatctg
     3841 ttcccgtcgt ccgcgaaccg gcccgcgccg gccgtgctat ccagcgcggc gtgagcgact
     3901 cccgccgggg gctggtccat ggcatcgccg cgtacttcct gtgggggatc gcgccggtgt
     3961 tctggaagct cttgcgcgac gtgtcgtggg tcgagagcct gtcccaccgc gtggtgtggg
     4021 gcgtgatcgc gttcggggtg atcgccgcgg cggtcggagc tggaccggag gtccgcgagg
     4081 cggcgcgcga ccgcggcacg ctggcggcga tggcgctgtc cggggtgatg ctcgtggcca
     4141 actggggaac gttcatctgg gcagtcgcgc gcggccacat cctcgacgcg agcctgggct
     4201 acttcatcat cccgctgatc tcggtcgggc tcggcatgct ggtgctcggc gagcggccgc
     4261 gccggctgca gtggatcgcg atcgccgccg cggcggtcgg cgtggcgatc cagacctggt
     4321 acgctggcgg gctgccgtgg atctcgatcg tggtcgccgt cagcttcggg ctctacggcc
     4381 tgatccgcaa ggtcgcgcgc gtcgactcgc tggtcggctc gacgctggag accgcgctgc
     4441 tggtgccggt cgcggcgagc tacctggtga tcctcgcgct gcgcggcggc ggccagctcg
     4501 gccacgcggc gaccggcacc cagctgctcc tgctgtcgac cggcgcggtc accgcggccc
     4561 cgctgctgct gttcaacagc gcggcgcgcc ggctgccgct gtcgacgctg ggcttcctgc
     4621 agtacctggg gccgacctgc cagttcatcc tcgcgatcgc ggtctacggc gagcggttcg
     4681 cgaccgagca gctgatcgcg ttcggcgcga tctggttcgg gctcgcggtg ttctcggtcg
     4741 acctggtccg cgcggcgcgc gcggccgcgc cggtcagccg aaccggccgg tgatgtagtt
     4801 ctcggtccgc tcgtcggacg gtgccgcgaa gatcttcgag gtcgcgtcgt actcgaccag
     4861 cgtgccggtg cggcgatcgt tcttggagtc gagctcggtg gtgaagaacg cggtgcggtc
     4921 gctgacccgg gcggcctgct gcatgttgtg ggtgacgatc acgatcgtga accggctctt
     4981 gagctcgcgc atcagctcct cgatccgggc ggtcgcgatc ggatcgagcg cgctgcacgg
     5041 ctcgtccatc agcacgacct cgggctcgac c
//
LOCUS       DASVOR010001304         1966 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_569080,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001304 DASVOR010000000
VERSION     DASVOR010001304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569080"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1190)
                     /locus_tag="VF516_24885"
     CDS             complement(<1..1190)
                     /locus_tag="VF516_24885"
                     /inference="COORDINATES: protein motif:HMM:NF024919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEX8111000.1"
                     /translation="MHPASEQPELWASTRKYYSQLEQELAAFREHDVSNQHYAADVVA
                     AARSVGYFRFCDLARNQQGDPNVIRDDWALALQTAQNMPTRKKELDDKLKFLPALSSS
                     NTDLSCQRECSLDEDAHPIPASMVIEGGEYYEWRFDSNRPLKDIELDNTNRKTGNGIS
                     LAVCNQPECTGQVHAVLTRKAAIREAIATWDVSTPPGSSIDIYMTPRIDGKWDSPLLV
                     AHWSNDRAVKTSVNSSSEHARIATDTLVVSHPDGADAIRLSLRLHSRPAAASPLVRSI
                     GLSVSGRDAPIVEDLDVPTISQMAQPDGDKKCSPTSVAMLTGYWASKTPGAWSPTTSD
                     VIDGVFDPAYGWGNWSFNVAYVNSASNGTMRAFVTRLRSLDELATLVTSTRPVMAIDA
                     ALPRR"
     gene            1323..>1966
                     /locus_tag="VF516_24890"
     CDS             1323..>1966
                     /locus_tag="VF516_24890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111001.1"
                     /translation="MALVLPLVAGSDPGIPGLPELAGRRAVCWTAAPAGAHDGAALAL
                     WIVDAETGKTIAAVAFDPTWSQLRPHYRPPYRPAVASATPAASWRTRSARCPRGCPAS
                     WPRSARRDLAHDPHQQFELALLLEIGNHLQGTYRERRAGPERCSAGMFARNSTSSSCA
                     GKSRNRRVSKAVARCTDDRTESLNCWIFIGSRSSNTCLTVSMIRWMSAVPAAPRR"
BASE COUNT          325 a          638 c          615 g          388 t
ORIGIN      
        1 cgccgcggca gtgctgcatc gatcgccatc accggccggg tactcgtgac cagcgtcgcg
       61 agctcgtcga ggcttcgcaa tcgcgtgacg aatgctcgca tcgttccgtt ggaagcagag
      121 ttcacgtacg cgacgttgaa cgaccagttc ccccaaccgt aggcaggatc gaacacaccg
      181 tcgatcacat cgctggttgt cgggctccag gcgcccggag tcttcgatgc ccaatatccc
      241 gtgagcatgg caaccgatgt cgggctgcat ttcttgtctc catctggttg ggccatctgc
      301 gagatcgtcg gcacatcgag atcttcgacg atcggcgcat cgcggccact gaccgacaat
      361 ccaatacttc tcaccagagg agatgcggcg gcaggcctcg agtgaagcct cagcgacaat
      421 cgaatggcat cggctccatc ggggtggctg acgaccagcg tgtccgttgc aattcgcgca
      481 tgctctgatg atgaattgac acttgtcttc actgctcgat cgttgctcca gtgcgccacg
      541 agaagcgggg agtcccactt cccatcgatc cgaggtgtca tgtaaatgtc gatcgaggaa
      601 ccgggtggcg tgctgacgtc ccatgtagcg atggcctccc ggatggcggc cttgcgggtg
      661 agcacggcgt gtacctggcc cgtgcactca ggttgattgc agaccgccag cgagattcca
      721 ttcccggtct tcctgttcgt gttgtccagc tcgatgtcct tcaaggggcg gttcgagtcg
      781 aatctccatt cgtagtattc gcccccttcg atcaccatgg acgccggtat cgggtgagca
      841 tcttcatcga ggctgcattc acgttggcat gacaggtcgg tgttactcga cgacagggca
      901 ggaaggaact tgagcttgtc gtcaagctcc ttcttcctcg tgggcatatt ttgagcagtt
      961 tgaagtgcga gggcccagtc atcgcggatg acgtttggat ctccttgctg attgcgtgcc
     1021 agatcgcaga atctaaaata tccgacgctg cgcgccgccg cgaccacgtc ggcggcatag
     1081 tgctggttcg agacatcatg ttcgcggaag gccgcgagct cctgctcgag ctggctgtag
     1141 tactttctgg tgctggccca gagctcgggc tgctcgctcg ccgggtgcat cagcccatga
     1201 ggggggacct ccccatgctg ggagtgtgcg ggccgctgtg ccgcgccatc gtggcccgca
     1261 gaggcagctc tgccagcgac cggcgacgag gccgaggctc gcgaagtgac ggtcccggcc
     1321 gcttggccct ggtgctgccg cttgtcgctg gctctgatcc gggaattccg ggtcttccag
     1381 agctcgccgg ccggcgcgcc gtgtgctgga ccgcggcgcc cgccggcgcg cacgacggcg
     1441 ccgcactcgc gctgtggatc gtcgacgccg agaccggcaa gaccatcgcg gcggtggcgt
     1501 tcgacccgac atggagccag cttcgcccac attaccgccc accttaccgc ccagccgttg
     1561 cctccgcgac gcccgccgcg agctggagga cgcgctcggc ccgctgcccc agaggctgtc
     1621 ccgcttcgtg gccacgaagc gcccggcgcg acctcgccca tgatccgcac cagcagttcg
     1681 agctggctct gctgctcgaa ataggcaatc acctccaggg cacgtatcgc gagcggcgcg
     1741 ccgggccgga gagatgctcc gccggaatgt tcgcccggaa tagcacctcg tcgagctgtg
     1801 ctggcaagag tcgcaatcgc cgagtcagca aggccgttgc acgctgcact gacgaccgga
     1861 ccgagtccct gaactgctgg atcttcatcg gatctcgatc atcgaacacg tgtctcaccg
     1921 tctcgatgat ccgctggatg tcggcggtgc cggccgcccc gcgccg
//
LOCUS       DASVOR010001305         2034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_305775,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001305 DASVOR010000000
VERSION     DASVOR010001305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305775"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VF516_24895"
     CDS             complement(<1..407)
                     /locus_tag="VF516_24895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111002.1"
                     /translation="MGTVLRTYCFLDALQPQQATFIGKTARGFLPVPVQASLWVEIAP
                     GIAINQVTDAALKATRVQPAVQVVERAYGLLEVHDHDKGEVLSAGATILRYLGVDEGQ
                     RLKPKVLTNQIIRGVEAYQTQIINRSSAGMMVLP"
     gene            526..>2034
                     /locus_tag="VF516_24900"
     CDS             526..>2034
                     /locus_tag="VF516_24900"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8111003.1"
                     /translation="MRFSRLACLLFALIASAPSGAHADVLGRVLKAARQQPGAGAPPP
                     QSPTLTEWAGEPRSITLPELLQITIRQAPNLQAAQIDIAVAEARIEQTWARNDWLARA
                     QLTGTRTGEVPQGNTTIDDEIRIGGTADLSRNLPTGGTIDLHAGTRYDRLNFTFTATN
                     PLTNEPLIQSSTLTTWTNDLSGAITQPLLRNRGRRLFDANEAKATLARDVSVLARRLA
                     AIQTVQTVISAYWDLVLAEQTVAITQASLDLARERLRVTRIGADGGKVPRSEIPAVLQ
                     IIATREEDVLNGELAVLNASIAMRRAAGMPIGAGDIGLRVPTDLDTKDQPFVLGELVD
                     RAFAASPELAQLTKQDASATIDVEVAENGLLPQLDAALTVGTSGLGDSFADATRDMAK
                     SKSIVVTGSLTFQQSLGRHDALGRTREQRELKHKLAVNAVDVRSQIAQTMSRAVAQLE
                     LARRRVTLSQRAIELANENIKIETDRFNLGKATNFDVLNRLEELRQAELRRAQ"
BASE COUNT          331 a          770 c          662 g          271 t
ORIGIN      
        1 ggcagcacca tcatgccggc cgagctgcgg ttgatgatct gggtctggta ggcctcgacg
       61 ccgcggatga tctggttggt caacaccttg ggcttcagcc gctggccctc gtcgacgccg
      121 aggtaccgga ggatggtcgc gccggccgac agcacctcgc ccttgtcgtg atcgtggacc
      181 tcgagcaggc cgtaggcgcg ctcgacgacc tggaccgcgg gctggacccg cgtcgccttg
      241 agcgccgcat cggtgacctg gttgatcgcg atgccgggcg cgatctcgac ccacagcgag
      301 gcctggaccg ggaccgggag aaagccgcgc gcggtcttgc cgatgaacgt cgcctgctga
      361 ggctggagcg cgtcgaggaa gcaatacgta cgcaggaccg ttcccatcgc tgccggccac
      421 gctaacacac gcggtttcag cagatccatg tcgcgtgcgg caattgcccg tcgcacgtgt
      481 cgcaccgtgc gggcggatcc ggtgcgtgcc atgatgcctc gatcgatgag gttttcacgg
      541 ctggcttgcc tgttgtttgc cctgatcgcc agcgccccgt cgggcgcgca cgccgacgtc
      601 ctgggccgcg tcctcaaggc ggccaggcag cagccgggcg ccggcgcgcc gcctccgcag
      661 agcccgacgc tcaccgagtg ggccggcgag ccccgctcga tcaccctgcc cgagctgctc
      721 cagatcacca ttcgccaggc gccgaacctg caagccgccc agatcgacat cgccgtcgcg
      781 gaggcgcgga tcgagcagac ctgggcgcgc aacgactggc tggccagggc ccagctcacc
      841 ggcacgcgca ccggcgaggt gccccagggc aacaccacga tcgacgacga gatccggatc
      901 ggcggaacgg ccgatctgtc gcgcaacctg ccgaccggcg gcacgatcga cctccacgcc
      961 ggaacccgct acgaccggct caacttcacg ttcaccgcca ccaacccgct gaccaacgag
     1021 ccgctcatcc agtccagcac cctcacgacc tggaccaacg acctcagcgg cgcgatcacc
     1081 cagccgctgc tccgcaaccg cggccgccgg ctgttcgatg ccaacgaggc caaggcgacg
     1141 ctggcgcgcg atgtcagcgt gctcgcccgg cggctcgccg cgatccagac cgtgcagacc
     1201 gtgatctcgg cgtactggga cctcgtgctc gccgagcaga ccgtggcgat cacccaggcg
     1261 agcctcgacc tcgcacgcga gcggctgcgg gtcacccgga tcggcgccga tggcggcaag
     1321 gtcccgcgca gcgagatccc cgcggtgctc cagatcatcg ccacccgcga ggaggacgtg
     1381 ctcaacggcg agctcgccgt gctcaacgcc tcgatcgcga tgcggcgcgc cgccggcatg
     1441 ccgatcggcg ccggcgacat cggcctgcgg gtccccaccg atctcgacac caaggaccag
     1501 ccgttcgtcc tcggcgagct ggtcgaccgc gcgttcgcgg cgagccccga gctcgcccag
     1561 ctcaccaagc aggacgccag cgcgacgatc gacgtcgagg tcgccgagaa cggcctgctg
     1621 ccccagctcg acgccgcgct gaccgtcggc acctccggcc tcggcgattc gttcgccgac
     1681 gccacgcgcg acatggccaa gtccaagtcg atcgtggtca ccggctcgct gacgttccag
     1741 cagtcgctcg gccggcacga cgcgctcggc cgcacccgcg agcagcgcga gctcaagcac
     1801 aagctcgcgg tcaacgccgt cgacgtccgc tctcagatcg cgcagaccat gagccgcgcg
     1861 gtcgcccagc tcgagctcgc ccgccgccgc gtcacgctgt cgcagcgcgc gatcgagctc
     1921 gccaacgaga acatcaagat cgagaccgac cggttcaacc tcggcaaggc gaccaacttc
     1981 gacgtgctga accggctcga ggagctgcgc caggccgagc tgcgcagggc ccag
//
LOCUS       DASVOR010001306         4151 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_218052,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001306 DASVOR010000000
VERSION     DASVOR010001306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218052"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VF516_24905"
     CDS             <1..267
                     /locus_tag="VF516_24905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX8111004.1"
                     /translation="TFVVDAPGGGGKRVAHSFEHYDRDTGISVFCAPSVKPGYFLYFD
                     PIDSLAPEIQARWTSPAERDRMIEDAIARAKVSQQASARASSVR"
     gene            380..1066
                     /locus_tag="VF516_24910"
     CDS             380..1066
                     /locus_tag="VF516_24910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111005.1"
                     /translation="MRTKNLVVTAMLIGGAAACSGGSDTVIGVRYDGVKVQCTSMGND
                     ESECVPVAPADGCNKVTVMWPPNHQMHHFTLAEACAPSDCGGDGSGSGSGSGSGSGSG
                     SGSGSGSGSGSGGIGIIGRTTSAAALPDGAKVTKIVVDELVDVMKGGDGHTADGGPTG
                     DLAVADDGKSFDLRSERQGGGDGRVYIVNWADDVTGTSGSCEFDVPHNQGPALGAVFS
                     GEVVTWPPAP"
     gene            complement(1210..1641)
                     /locus_tag="VF516_24915"
     CDS             complement(1210..1641)
                     /locus_tag="VF516_24915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEX8111006.1"
                     /translation="MASRRDDLETLRTAVRNAGLRATPSRIAVLRLLRAAGSPVSHSD
                     VVAELSSQAWDPATIYRNLTDLSDVGLARRTDVGDHVWRYEAITGGHQASAHPHFVCT
                     ECGSVECLPDLDYVVTRAKTPRAVRLKRVEVQLRGPCDACL"
     gene            1745..2179
                     /locus_tag="VF516_24920"
     CDS             1745..2179
                     /locus_tag="VF516_24920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111007.1"
                     /translation="MTIDPAAAFQRFADALNRARDPASLRAAVTDDVRLDRHAPGERT
                     TAPVAESFAGVAEVERWFARMPAGVQFALAGAAWTEPDGGWGVEYAYDAGDFHHGGIW
                     IARLAGDGRIARLSHHPFALRDAPGPPGAPHAHGGSGHTHGG"
     gene            2196..3656
                     /locus_tag="VF516_24925"
     CDS             2196..3656
                     /locus_tag="VF516_24925"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8111008.1"
                     /translation="MRPAAPGCLWITASITASITASIAASIACLTSCGMPAPKPPLAP
                     LDPRDLPANIDRIAGEVLADTGVPSASVAAVSDGKIVYVHAYGNARLDPPTPAAPGMR
                     YGIGSISKQLTAAAVLLLAQDGKLTLDDPVGKYVPNLTRGDVITIRQILSHTSGYPDY
                     APQDYIVPEWERPVSPDALLDRWARKTLEFEPGTKWQYSNTNFVIAGLIVEKVAGVPL
                     VDFLTERVFKPLGMASVTNTDRQKLGDHDAQGYFRRALGPLHPAPHEEPGWMYAAGEL
                     AMTAEDLAKWDAAVIQQAILAPESWRQLETEVVLASGAGTRYGLGVSVGLTQGHRELG
                     HGGEVSGFVASNLVWPDDKLAVAVLTNQDASGAASEIATRVRDALFRAISPASVESDR
                     RVKQVLAELADKKLDKTRLTANLASYFTDEAIAEFADTLRPLGALDAVEQLSSGKRGG
                     MTFRRYKAKYGDKAMSISVYETDDGKLEQFLLSKPE"
     gene            3676..>4151
                     /locus_tag="VF516_24930"
     CDS             3676..>4151
                     /locus_tag="VF516_24930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111009.1"
                     /translation="MTHRRPSRDRRPGGAIRSLPFRHNVKPALGFEIFRLSQLYERAD
                     QHRIDHALDAPQRPEFHTIYVGLRGKGRLIVDFTPVPLGAGTLTFVARGRVQQFVPDR
                     AVDAWMLLFAPEFVLAGGDAPDPLALPATLAPSWASPAIAMPPDEARELIALAEQI"
BASE COUNT          617 a         1451 c         1496 g          587 t
ORIGIN      
        1 accttcgtgg tcgatgcgcc gggcggcggc ggtaagcggg tggcgcacag cttcgagcac
       61 tatgatcgcg acaccggcat ctcggtgttc tgcgcaccgt cggtcaagcc gggctacttc
      121 ctctacttcg acccgatcga ctcgctggcg ccggagatcc aggcgcggtg gacgagcccc
      181 gccgagcgcg atcggatgat cgaggacgcg atcgcgcgcg caaaggtcag ccagcaggcg
      241 agcgcgcggg cctcgagcgt gcgttgaggt ttcaggccgt tgcagacggc tgcgtggctg
      301 cgcgcacgta cgaagcctgc acaatcaatt agttgcgacc tcgccgaggg gcctccccac
      361 tgtagagcca ggaggcttca tgcgaacgaa gaacctggta gtcacagcga tgctgatcgg
      421 cggggcggcg gcgtgctcgg gcggttcgga cacggtgatc ggcgtgcgct atgacggcgt
      481 caaggtccag tgcacctcga tggggaatga cgaatcggag tgcgtgccgg tcgcccccgc
      541 cgacgggtgc aacaaagtga ccgtgatgtg gccgccgaac caccagatgc accacttcac
      601 gctcgccgag gcctgcgccc cgtccgactg cggcggcgat ggcagcggaa gtggcagcgg
      661 cagcggcagc ggaagtggca gcggtagcgg aagtggcagc gggagtggca gcggcagcgg
      721 cgggatcggc atcatcggcc gcacgacctc ggccgcggcg ctccccgatg gggcaaaggt
      781 cacgaagatc gtcgtcgacg agctggtcga cgtcatgaag ggcggcgatg gccacaccgc
      841 ggacggcggt cccacgggcg acctcgcggt cgcggacgat gggaagtcct tcgacctgcg
      901 ctccgagcgg cagggcggcg gcgacggccg ggtctacatc gtcaactggg ccgacgatgt
      961 cacgggcacc agcggatcgt gcgagttcga tgtcccgcac aaccagggcc cggccctcgg
     1021 cgcggtcttc agcggcgagg ttgtgacctg gccgccggcc ccgtgatgat cgtctgatcc
     1081 tgccgccggg tgggctggtc gtgcgaggaa ccgatgccgg cgcggccgtt ttgcgtcacg
     1141 gccgcacggc atccgggtgc gccggtccac ccggtggtgg atctttttcg aggctggccc
     1201 aggtgcgggt tacaggcagg cgtcgcacgg tccgcgcagc tggacctcga cccgcttgag
     1261 ccggaccgcg cgcggcgtct tggcgcgcgt gaccacgtag tcgaggtcgg gcaggcactc
     1321 gacgctgccg cactcggtgc acacgaagtg cgggtgggcc gacgcctggt ggccgccggt
     1381 gatcgcctcg tagcgccaca cgtggtcacc gacgtcggtc cggcgcgcca ggccgacgtc
     1441 cgagagatcg gtcaggttgc ggtagatcgt cgcggggtcc cacgcctggg aagagagctc
     1501 ggcgaccaca tcgctgtgcg acaccggcga gccggccgcg cgcagcaggc gaagcaccgc
     1561 gatccgcgac ggggtcgcgc gcagcccggc gttgcgaacc gcggtccgca aggtctcgag
     1621 atcgtcccgg cgagatgcca tcgtggccaa ctgtacgcga gcccggcgcg gcgtgcaagt
     1681 acatgcaact agattgcgtt tggcgcacgg tgcgcggcgg cccggggtct ggcatcgtgg
     1741 cagcgtgacg atcgatcccg ccgcggcgtt tcagcggttc gccgatgcgc tcaaccgcgc
     1801 gcgcgatccg gcgtcgctcc gcgcggccgt gaccgacgat gtgcggctcg atcgccacgc
     1861 cccgggcgag cgcaccaccg cgccggtcgc ggagtcgttc gcgggtgtcg ccgaggtcga
     1921 gcgctggttc gcgcgcatgc cggcaggcgt gcagttcgcg ctcgccggcg cggcgtggac
     1981 tgaaccggac ggcggatggg gcgtcgagta tgcctacgat gccggggact ttcaccacgg
     2041 cgggatctgg atcgcgcggc tcgccggcga tggccggatc gctcggctgt cgcatcaccc
     2101 gttcgcgctg cgcgacgccc cggggccgcc gggggctccc cacgcgcacg gcggttctgg
     2161 acacacgcac ggcggatagt cgtacagtgc gcgcgatgcg acccgccgcg cctggatgtc
     2221 tgtggatcac cgcctcgatc accgcctcga tcaccgcctc gatcgccgcc tcgatcgcct
     2281 gcctgacgtc gtgcggaatg cccgcgccga agcccccgct ggcgccgctc gatcccaggg
     2341 atctgccggc gaacatcgat cggatcgccg gcgaggtact cgccgacacc ggcgtcccca
     2401 gcgcgtcggt cgccgcggtg tccgacggca agatcgtcta cgtccacgcc tacggcaacg
     2461 cgcggctcga cccgccgacc ccggcggcgc ccgggatgcg ctacggcatc ggctcgatca
     2521 gcaagcagct gaccgcggcc gccgtgctgc tgctcgccca ggatggcaag ctcacgctcg
     2581 acgatccggt cggcaagtac gtcccgaacc tgacgcgcgg cgatgtgatc acgatccgtc
     2641 agatcctgtc gcacacctcg ggctaccccg actacgcgcc gcaggactac atcgtcccgg
     2701 agtgggagcg gccggtgtca ccggacgcgc tgctcgaccg ctgggcgcgc aagacgctgg
     2761 agttcgagcc cggcaccaag tggcagtaca gcaacaccaa cttcgtgatc gccggcctga
     2821 tcgtcgagaa ggtcgccggc gtgccgctgg tcgacttcct caccgagcgc gtgttcaagc
     2881 cgctcggcat ggcgtcggtg accaacaccg atcgccagaa gctcggcgac cacgacgccc
     2941 agggctactt ccgccgcgcg ctcgggccgc tccacccggc gccgcacgag gagccgggct
     3001 ggatgtacgc cgccggcgag ctcgcgatga ccgccgagga cctcgcgaag tgggacgccg
     3061 cggtgatcca gcaggcgatc ctggcgccgg agtcgtggcg ccagctcgag accgaggtcg
     3121 tgctcgcgag cggtgccggc acgcgctacg gcctgggcgt cagcgtcggg ctgacccagg
     3181 gccaccgcga gctcggccac ggtggcgagg tctcggggtt cgtcgcgtcg aacctggtgt
     3241 ggcccgacga caagctcgcg gtcgccgtgc tgaccaacca ggacgcgtcg ggcgcggcca
     3301 gcgagatcgc gacccgggtg cgcgacgcgc tgttccgcgc gatctcgccg gccagcgtcg
     3361 agagcgaccg tcgggtcaag caggtgctgg ccgagctcgc cgacaagaag ctcgacaaga
     3421 cccggctgac cgcgaacctc gccagctact tcaccgacga ggcgatcgcc gagttcgccg
     3481 acacgctgcg cccgctcggc gcgctcgacg cggtcgagca gctgtcgtcc ggcaagcgcg
     3541 gcggcatgac gttccggcgc tacaaggcga agtacggcga caaggcgatg tcgatcagcg
     3601 tgtacgagac cgacgacggc aagctggagc agttcctgct gagcaagccc gaatgacccg
     3661 gcccgatcga ctggaatgac gcaccggcga ccatcccgcg atcggcgtcc cggcggcgcg
     3721 atccgcagcc tgccgtttcg ccacaacgtc aagccggcgc tcggcttcga gatcttccgg
     3781 ctgtcgcagc tctacgagcg cgccgaccag catcggatcg accacgcgct cgacgccccg
     3841 cagcgccccg agttccacac gatctacgtc gggctgcgcg gcaagggccg gctgatcgtc
     3901 gacttcaccc cggtgccgct cggggccggc acgctgacct tcgtcgcgcg cggccgggtc
     3961 cagcagttcg tgcccgaccg cgcggtcgat gcctggatgc tgctgttcgc gcccgagttc
     4021 gtgctcgccg gcggcgacgc gcccgatccg ctggcgctgc cggcgacgct cgcgccgtcg
     4081 tgggcgagcc ctgcgatcgc gatgccgccg gacgaggctc gcgagctgat cgcgctcgcc
     4141 gagcagatcg a
//
LOCUS       DASVOR010001307        10354 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421870,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001307 DASVOR010000000
VERSION     DASVOR010001307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421870"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VF516_24935"
     CDS             <1..654
                     /locus_tag="VF516_24935"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8111010.1"
                     /translation="VVATDLGVPAAAGALWTAARAGGPVDILINNAGFGYFRPFRAAD
                     WARDAELVQLNIASLVELSRRFVDDRSTGFPHDRAYLVNIASIGAYQAVPHMAVYASS
                     KAFVRNFTEALHDELRGSPISATCVCPGGTRTDFHAMAGAGNYGWLADSSMMTAEAVA
                     QVAVRAMRRGKRNVIPGALNKLACWSVRLVSRRLAAWMSTRVLGRPRTAALPARTAA"
     gene            728..1843
                     /gene="gcvT"
                     /locus_tag="VF516_24940"
     CDS             728..1843
                     /gene="gcvT"
                     /locus_tag="VF516_24940"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX8111011.1"
                     /translation="MERKRTPLWQVHKQLGARLIDFGGWDMPVTYPAGTIKEHKAVRE
                     AVGLFDVSHMGEAVLRGPRVREAVERLTTNAVASAPPGKAVYTLLCRPDGGVVDDCIF
                     YKRSDDEFFVIVNASNTAKDLDWFRDHTHSLCDIDDISDQTALIAVQGPKAVGLVDQL
                     AGGSLAGLPSFGFANAVVAGVECMAARTGYTGEDGFELACAADDAVALWTALMENLPP
                     GGLPIGLGARDTLRLESRLPLYGNDLDDDHTPLEAGLGWAVKLDREFIGAQALRDQKQ
                     RGLTRQLAGFKIAEDARAIARHGYAVVDRSLGAGEAAVIGTVTSGGPGICVGGSIGLA
                     YVPVARAKPGTELTIDCRGKDTPATVVSGKFYKRTQP"
     gene            1840..2226
                     /gene="gcvH"
                     /locus_tag="VF516_24945"
     CDS             1840..2226
                     /gene="gcvH"
                     /locus_tag="VF516_24945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEX8111012.1"
                     /translation="MTSFPTDLRYTHDHEWLRATGSAWRVGITQFAVDQLGDITLVDL
                     PKEGDLVTKGQRFGTIESVKSVSDLYAPVSGKVTAVNASLKDAPEQVNNEPYGAGWMI
                     ELEPTEKAELSELMAPDAYAQHVAAQ"
     gene            2308..5136
                     /locus_tag="VF516_24950"
     CDS             2308..5136
                     /locus_tag="VF516_24950"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8111013.1"
                     /translation="MLVFPPFELDVADERLWKAGEELPLRRKPFAILRYLAEHPMRLV
                     TQQELVDAVWGEVAMSDSVLRTHIRDLRRVLGEDLIETVVGRGYRFLVEVTGAAPAPV
                     RDAGIPAPAIAGREDKLEILAAALREALAHRRQMVFLVGDAGIGKTTVVDALLEHAAP
                     SATIARGACVEQYGAGEAYLPVLDALGRACRGAEGERIVAVLARHAPSWLTQLPGTVS
                     DDQLAELQKKLQGVTQARMLRELADALEVLGTERPFVLALEDLHWSDTSTVELLAMLG
                     RRREPARLLVIGTLRRTELPKAHPLARVIGELVSHRQAMQLALEPLPVAAVDRYLAQR
                     FRGHAFPDGFAAALHTTTAGHPLFLVAMLDDLERRDLVRPIDGRWALAVPLAEVAARR
                     PESVMQLIDIQVDRLGAAEQRVLEAASIAGATFASAAVAAALEVPLADVDAACELLAD
                     EHPYLRRLEAEAWPDGSLPSRYGFTHAIYQHAAAARVSPGAERQWRRRVAECLEAGHR
                     AAPASIAAELAMHFDRAPLPAKALAYYVMAGERSIRRSGAADALRNLQRAQELVERLP
                     EGIERDAAELGLLQHLGPTLMLTKASPTLATYQRASELASRLGAGPDLARALVGMLYS
                     RTAHGDLREVGASAAPVLDVVQRTGDPALTGHVELTLATAASLRGRLDEAKNWADAIR
                     SSFDAQSRRPVAWSDYLPDPCLVAYGVSSIVVWLLGFPDAALVHARAGLVAAESIQDP
                     VSIVLLLWNMTMVHMWRGEAALVREIAERGLAIARRFDFELVRCRAEIVIPWASARLG
                     DLPESPDALAISLSELRTFSVVEGVIVALPCIEIFVQAGLRAQALDEIERSLAIVREI
                     DARALEPELLRLRGEIVKADDPGEARRCFTAALELAESQRSRSFALRAATSLARLGES
                     RAELRRIYDTFTEGFDTGDLVEARALLA"
     gene            complement(5163..5597)
                     /locus_tag="VF516_24955"
     CDS             complement(5163..5597)
                     /locus_tag="VF516_24955"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8111014.1"
                     /translation="MANPRWIVVGTSFSERAQHALAYAIEVAARTGARIALVHAYEDS
                     IDPPVDVAPGLRSRLEAAIERSGAARLGVHVEPVLRRGAPWDKLRNVATDLGADLLVI
                     GASDRHDLAADALTTRAIASAARSVLVIAMHDPPCHDARPRP"
     gene            complement(5607..5837)
                     /locus_tag="VF516_24960"
     CDS             complement(5607..5837)
                     /locus_tag="VF516_24960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111015.1"
                     /translation="MNASRGSSMRLLAIIPLVLLAVSARARSDARFEIYTWFDDLARA
                     GRWIAGAVRRHRPALDRGAGEIDLADLHPEER"
     gene            6223..7335
                     /locus_tag="VF516_24965"
     CDS             6223..7335
                     /locus_tag="VF516_24965"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8111016.1"
                     /translation="MQIVNPDGHTPQRAAWQPISQLPPALALRQLMFGHRVTRIITVA
                     AQLRLADAMDDTPRSVAALAAAVGTDTAALHRLLYALASIGLVTVSAPDQFVLTPVGA
                     CLRSDAPHGLRSWALMESAEYYQTAWDHLLTSVRSGAPAFEGAVGQSFYDYLDRHPVD
                     GANFNQTMGEVTTVIVDAVLTAYDFASVEQVVDVGGGYGKLLTSLLQRYPTMRGVLLD
                     TPAVIERARPQVQAAGVADRCELVGGDFFADLPAGGDLYLLSRILMDHDDEGSVRLLR
                     NCRRVMTERGRVHIIQIVLPPPDADAAHHLLFDGAMSNLNMFVLGLGAERTEEQYRAL
                     LARAGLAVTQIIPTRALMSIIEARSADRIEIPEHSI"
     gene            7841..9892
                     /locus_tag="VF516_24970"
     CDS             7841..9892
                     /locus_tag="VF516_24970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111017.1"
                     /translation="MARSLNTGLLFQANASGSGSVASWASTWNTWTPAVQIPAVSGVN
                     ANGDVWTASSAYSGLEYFSARTVTASGVVPYQSATGIAASRAADLLTSGKFDYLDRWA
                     IPASEAGDGQSIAVDEGGTSLWLAASSASGTRPYVYDFPDCRSGSPGDTNCARWMPGN
                     SGNDYVDLLSDGGDDEVGMSAYSHKTVVVNPCTHHALVSYLETNGTVTNMKVKAVSRT
                     GAITGRWTFPTSQQPNDRCTGTTTFTCSGGNRYLCPPPASGADNCGYNRYVPRVQLAT
                     KVVAETSQCLLYAGWDESYTSSVGQRLRAVMAIIDVTASPSKPEAPARVIHSTPPDGT
                     QYYGSTPVISRYGDSIGWFFVATAPPYDNNHILGYVSPVHNMDLMTSTVVFVSDTFAN
                     TWMGDNMSQLLGGLPGGDLFATWPESINGCSQIAGARVTPPYSPSACQAASEQYGIVA
                     GSTFGYAPPDVRSWWIGAGCTTSPLYTQNLCQRASDVYGIIANQTFGTAPASVRDWWT
                     SNGCNTTPTPKNTCERASQLYGITNNVSWGFAPSSVQTWWTANNCNAQQTVPVAACQR
                     AADLYGIVANETWGFAPDDIKDWWRTHSCNARAQSTNACQRASERYGIIANKTFGFAP
                     ASVQTWWISNGCNDVPLKTSDACQVASNLFGIDAGVSFAFAPDDVQAWWKGAGCNAHP
                     Q"
     gene            complement(9989..>10354)
                     /locus_tag="VF516_24975"
     CDS             complement(9989..>10354)
                     /locus_tag="VF516_24975"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8111018.1"
                     /translation="QLTVPPKLRELTIMAVGALNQADYEWAQHEAEFTKAGGTAAQLA
                     ALKNPPAAMKNDKLFDEPERATLALTIEMTRSIKVSDATMKRIRAVLPLDQVIELIGT
                     IAGYNMASRFLVATGVELE"
BASE COUNT         1615 a         3517 c         3551 g         1671 t
ORIGIN      
        1 gtcgtcgcga ccgacctcgg ggtccccgcc gcggccggcg cgctgtggac cgccgcgcgc
       61 gccggcggcc cggtcgacat cctgatcaac aacgccgggt tcggctactt ccgcccgttc
      121 cgcgccgccg actgggcgcg cgacgccgag ctggtccagc tcaacatcgc gtcgctggtc
      181 gagctgtcgc gccggttcgt cgacgaccgc agcaccggct tcccgcacga ccgcgcctac
      241 ctggtcaaca tcgcgtcgat cggcgcctat caggccgtcc cgcacatggc ggtctacgcc
      301 agctcgaagg cgttcgttcg caacttcacc gaggcgctgc acgacgagct gcgcggctcg
      361 ccgatcagcg cgacctgcgt ctgtccgggc ggcacccgca cggactttca cgccatggcc
      421 ggcgccggca actacggctg gctcgccgac agctcgatga tgaccgccga ggcggtcgcg
      481 caggtcgccg tccgggcgat gcgcaggggc aagcgcaacg tcatccccgg cgcgctcaac
      541 aagctcgcct gctggtcggt ccgcctggtc agccgccggc tcgccgcctg gatgtcgacc
      601 cgcgtcctcg gcagaccgcg gaccgccgcg ctcccggccc gcaccgccgc gtgacgcggt
      661 gcttcgcgcc gccgatctcg cccccggcgc gcccccggtt tgccgggcgt gctagtgtcc
      721 gcccgctatg gaacgcaagc gaacgccgct gtggcaggtg cacaagcaac tcggcgcgcg
      781 cctgatcgat ttcggcggct gggacatgcc ggtcacctac ccggccggga cgatcaagga
      841 gcacaaggcg gtccgcgagg cggtcggcct gttcgacgtc tcgcacatgg gcgaggcggt
      901 gctgcgcggg ccgcgggtcc gcgaggcggt cgagcggctc accaccaacg cggtggcgtc
      961 ggcgccgccc ggcaaggcgg tctacaccct gctgtgccgc cccgacggcg gggtggtcga
     1021 cgactgcatc ttctacaagc gctccgacga cgagttcttc gtcatcgtca acgcgtcgaa
     1081 cacggccaag gacctcgact ggttccgcga ccacacccac agcctgtgcg acatcgacga
     1141 tatcagcgac cagaccgcgc tgatcgcggt ccagggcccc aaggcggtcg ggctggtcga
     1201 tcagctcgcc ggcggcagcc tcgccgggct gccgagcttc ggcttcgcca acgcggtggt
     1261 cgccggcgtc gagtgcatgg ccgcccgcac cgggtacacc ggcgaggacg gcttcgagct
     1321 ggcgtgcgcc gccgacgacg cggtggcgct gtggaccgcg ctgatggaga acctgccgcc
     1381 cggcggcctg ccgatcggcc tgggcgcccg cgacacgctg cgcctggagt cgcggctgcc
     1441 gctgtacggc aacgacctcg acgacgacca caccccgctc gaggccggcc tgggctgggc
     1501 ggtcaagctc gaccgcgagt tcatcggcgc ccaggcgctg cgcgaccaga agcagcgcgg
     1561 cctgacccgc cagctcgccg gcttcaagat cgccgaggac gcgcgcgcga tcgcgcggca
     1621 cggctacgcg gtggtcgacc gctcgctcgg cgccggcgag gccgcggtga tcggcacggt
     1681 caccagcggc ggcccgggca tctgcgtcgg cggctcgatc ggcctggcct atgtcccggt
     1741 cgcgcgggcc aagccgggca ccgagctcac gatcgattgc cgtggcaagg acacccccgc
     1801 gacggtggtg tccggcaagt tctacaagcg gacccaacca tgaccagctt ccccaccgac
     1861 cttcgctaca cccacgatca cgaatggctc cgcgcgaccg gctcggcctg gcgcgtcggc
     1921 atcacccagt tcgccgtcga tcagctcggc gacatcaccc tcgtcgatct gcccaaggag
     1981 ggcgacctgg tcaccaaggg ccagcggttc ggcacgatcg agagcgtcaa gagcgtgtcc
     2041 gacctgtacg cgccggtgtc cggcaaggtc accgcggtca acgcttcgct gaaggacgcc
     2101 cccgagcagg tcaacaacga gccgtacggc gcgggctgga tgatcgagct cgagccgacc
     2161 gagaaggccg agctgtccga gctgatggcg cccgacgcct acgcccagca cgtcgcggcc
     2221 cagtagcgat cggcgcgaag cggctgtcca ctccatcctc gcttgacggt gcgggcgggg
     2281 tgaccgattc tcgccgcgga ggtggccgtg ctggtgtttc cgccgttcga gctcgacgtg
     2341 gccgacgagc gcttgtggaa ggccggcgag gagctgccgc tccggcgcaa gccgttcgcg
     2401 atcctgcgct acctggcgga gcatcccatg cgcctggtca cccagcagga gctggtcgac
     2461 gccgtgtggg gcgaggtcgc gatgagcgac agcgtgctgc gcacccacat ccgcgatctg
     2521 cggcgggtgc tcggtgagga tctgatcgag accgtggtcg gccgcggcta tcggttcctc
     2581 gtcgaggtca ccggggctgc gccggcgccg gtccgcgacg cgggcatccc ggcgccggcg
     2641 atcgcaggcc gcgaggacaa gctggagatc ctggcggccg cgctgcgcga ggcgctcgcg
     2701 caccggcggc agatggtgtt cctcgtcggg gacgccggga tcggcaagac gaccgtggtc
     2761 gacgcgctgc tcgagcacgc cgcgcccagc gccacgatcg cgcggggcgc gtgcgtcgag
     2821 cagtacggcg cgggagaggc atacctgccg gtgctcgacg cgctcggccg ggcgtgccgg
     2881 ggggccgagg gcgagcgcat cgtggcggtg ctggcgcgcc acgcgccgag ctggctgacc
     2941 cagctgccgg gaacggtctc ggatgaccag ctggcggagc tgcagaagaa gctgcaaggg
     3001 gtgacccagg cgcggatgct gcgcgagctg gccgatgcgc tggaggtcct ggggaccgag
     3061 cgcccgttcg tcctggcgct cgaggacctg cactggtccg atacctcgac ggtggagctg
     3121 ctcgccatgc tcggccggcg gcgcgagccg gcgcggctct tggtgatcgg gacgctgcgc
     3181 cgcacggagc tgccgaaggc gcatccgctg gcgcgggtga tcggcgagct ggtgtcgcac
     3241 cgccaggcga tgcagctcgc gctggagccg ttgccggtcg ccgcggtcga tcggtacctc
     3301 gcgcagcggt tccgcggtca cgccttcccc gacgggttcg ccgccgcgct gcacacgacg
     3361 acggccgggc acccgctgtt cctggtggcg atgctcgacg acctcgagcg gcgggatctg
     3421 gtgcggccga tcgacggccg ctgggcgctc gcggtgccgc tggccgaggt cgcggcgcgg
     3481 cgtccagagt cggtgatgca gctgatcgac atccaggtcg atcggctggg tgcggccgag
     3541 cagcgggtgc tggaggcggc gagcatcgcc ggggcgacct tcgcatcggc cgcggtcgcc
     3601 gctgcgctgg aggttccgct cgccgatgtc gatgcggcgt gcgagctgct cgcggacgag
     3661 cacccgtacc tgcgccggct ggaggcggag gcctggcccg acggcagcct gccgtcgcgc
     3721 tacggcttca cccacgcgat ctaccagcac gcggcggccg cgcgcgtgtc gcccggagcc
     3781 gagcggcagt ggcggcgccg cgtcgccgaa tgcctcgagg ccggccaccg cgcggccccg
     3841 gcatcgatcg ctgccgagct ggcgatgcac ttcgatcgcg caccgctgcc cgccaaggcg
     3901 ctcgcgtact acgtgatggc gggtgagcga tcgatccggc gctcgggtgc tgccgatgcg
     3961 ctccgcaacc tccagcgcgc ccaggaactg gtcgagcggc tcccggaggg catcgagcgc
     4021 gacgcagccg agctcggctt gctccaacac ctcggtccca cgttgatgtt gacgaaagcg
     4081 tcgcccacac ttgcgaccta ccagcgagcg agcgagctcg cgtctcggct cggcgccggt
     4141 cccgatctgg cgcgtgcgct ggtggggatg ttgtactccc gcaccgcgca cggcgacctc
     4201 cgcgaggtcg gtgccagcgc cgcgccggtg ctcgacgtcg tacagcgcac cggagatcct
     4261 gcgctcacag ggcacgtgga gctcacgctg gcgacggccg cttcgctgcg tggcagactg
     4321 gacgaggcga agaactgggc tgacgccatc cggtcatcgt tcgatgcgca gagccgtcgc
     4381 ccggtggcct ggagcgacta cctgcccgat ccttgcctcg tggcgtatgg cgtgtcgtcc
     4441 atcgtcgtct ggttgctcgg ctttcccgac gcggccctgg tgcacgctcg cgccgggctg
     4501 gtggccgccg agtcaatcca ggatccggtt tcgatcgtgt tgttgctgtg gaacatgacc
     4561 atggtccaca tgtggcgcgg cgaggccgcg ctggtgcgcg agatcgcgga gcgtgggctc
     4621 gcgatcgcga gaaggttcga cttcgagtta gtgaggtgtc gcgccgagat cgtcatcccc
     4681 tgggcgtccg cgaggctggg cgatctgcct gagagtccgg acgcactcgc gatctcgttg
     4741 tcagagctcc ggacgttctc tgtggtcgaa ggggtgatcg tcgcgctgcc ctgcatcgag
     4801 atcttcgtcc aagccggact ccgtgcacag gcgctcgacg agatcgaacg cagcctcgcg
     4861 atcgtgcgcg agatcgacgc acgcgccctg gagcccgaac tgctccggct acgcggcgag
     4921 atcgtgaagg ccgatgatcc cggcgaggcg cggcggtgct tcactgcggc gctcgagctc
     4981 gccgagagcc agcgatcgcg gtcgttcgcg ctgcgcgcgg cgaccagcct ggccaggctc
     5041 ggagagtcgc gcgccgagct gcggcggatc tacgacacgt tcacggaggg cttcgacacc
     5101 ggcgatctcg tcgaggcgcg tgccctcctc gcatgacggc gccgaccggg tggcacatgt
     5161 gatcacgggc gcggccgcgc atcatggcat ggcggatcgt gcatcgcgat cacgagcacc
     5221 gaccgcgcgg cggacgcgat cgcgcgggtc gtcagcgcat cggcggccag gtcgtgccgg
     5281 tcgctggcgc cgatcacgag cagatcggcg ccgagatccg tcgcgacgtt gcggagcttg
     5341 tcccacggtg ccccgcgccg gagcacgggc tcgacgtgaa caccgagccg cgcggcaccc
     5401 gatcgctcga tcgcggcctc gaggcgtgag cgcagccccg gcgcgacgtc gaccggcgga
     5461 tcgatcgagt cctcgtaggc gtggacgagc gcgatgcgcg caccggtccg ggccgcgacc
     5521 tcgatggcgt acgcgagcgc gtgctgggcg cgctcgctga agctcgtacc gacgacgatc
     5581 catcgtgggt tcgccatggc gccgacctag cgctcctctg gatggaggtc ggcgaggtcg
     5641 atctcgccgg cgccccggtc cagcgccggg cgatgacggc gcaccgcgcc cgcgatccac
     5701 cgcccggcgc gcgcgaggtc gtcgaaccag gtgtagatct caaatcgggc atcggatctg
     5761 gcgcgggcag agactgcgag gagaacaagg gggatgatgg cgagcaagcg catggacgac
     5821 ccccgggatg cgttcatgtc tcggaggcta agagcgcatg cgaagcgccg ccgtcagatg
     5881 ctcgtcagat caccgtcgaa tggccggtag cgcgtcgaac cggcgtgaaa acacgccggc
     5941 actcgacgtc attcgagctg cgcgcggcgg tgatcctggc gaggctcggc gagtcgcgag
     6001 ccgagctgca tcggatcgat cgaaccgctc caaatactcg atttgacgcc gatttgacgc
     6061 cgatctcaca tccggtggcg gtgctctgcc ctacaacgcg agggacgcgt gggagcggcg
     6121 ccagcgtcat ctcggatgtg accactccgg atctgcgggc gcaggcacct gccggcgctc
     6181 tggccgaggt cgtgccgcgg tttgaaacag gagagaatct caatgcagat tgtcaacccg
     6241 gatgggcaca ctccacaacg cgccgcctgg caaccgatca gtcagcttcc cccggctctg
     6301 gccttgcggc agctgatgtt cgggcatcgg gtgacgcgca tcatcacggt ggccgcccag
     6361 ctgaggctgg ccgatgcgat ggatgacacg ccgcgcagcg ttgctgccct ggccgccgcc
     6421 gtgggtaccg atacggctgc cttgcaccgg ctattatatg ccctcgccag tatcggtctc
     6481 gtgaccgtat ccgcgcccga tcaatttgta ctgaccccgg tgggcgcgtg cctgcggagc
     6541 gatgcccccc atgggctgcg ctcgtgggcg ctgatggaga gtgccgagta ttaccagacc
     6601 gcatgggatc atctcttgac cagcgtgcgc agcggggcac ccgccttcga aggcgcggtc
     6661 ggccagtcgt tttacgacta cctggatcga caccccgtgg acggtgcgaa cttcaaccag
     6721 acgatgggag aggtcaccac cgtgatcgtc gatgccgtgc tgactgccta tgactttgcg
     6781 tcggtggagc aggtcgtcga cgtcggcggt ggctacggca agctgttgac cagcctcttg
     6841 cagcgctatc ccacgatgcg cggcgtgctc ctcgacacgc cggccgtgat cgagcgggcg
     6901 agaccgcagg tccaggccgc cggagttgcc gatcgctgcg agctggttgg cggggacttc
     6961 tttgccgact tgccggccgg cggcgatctc tacctgcttt cgcgcatcct gatggaccac
     7021 gacgacgaag gcagtgtgcg cctcctgcgc aattgccgcc gggtgatgac cgaacgaggg
     7081 cgagtacaca tcatccagat cgtgctgccg ccacccgacg cggacgccgc acaccacctg
     7141 ctgttcgatg gcgccatgtc caacctcaac atgttcgtgc tgggtctggg cgcagagcga
     7201 acggaagaac aatatcgcgc gctgctcgcg agagccggcc tcgcggtcac gcagatcatc
     7261 ccgacccgcg ccttgatgag tatcatcgag gccagatcgg cggatcgcat cgaaatacct
     7321 gaacactcga tttgatgtcg atttgacatc gatctcacat caggtggcag cgccccgcgc
     7381 cagcgtgaat catcacactc gatagcgctc actcacagca atgcgaccag aggattcatc
     7441 catcatgaaa acagcgattt tctgtatagg gtcatacttc ttcgttgcgt gttcgcccac
     7501 tccgttttca actacacggg agcggatcgg agttgcccag caggggctga gcggcccggt
     7561 cgggagaatc tgcacaacgt cagcggattg tgggggtggc gatgtcggtt gctacgacgc
     7621 ccactgcagc tgcgctccgg gcttcatcgc gtgtggtacg ggatgtgtcg acatcgcccg
     7681 ggacgcaagt cactgcggtg cgtgtgagag ctcctgcgca gcgggacagg tctgtgcggc
     7741 cggcgtctgt caatcgaccg cggcgccgac cacggagccc atcggcgctt cggcctcggt
     7801 gaatgtcacg ggagcttgtc acggcgggtt cgagagtcac gtggcacgct cgctcaacac
     7861 ggggctcctc ttccaggcga acgcttccgg cagtggctcg gttgcgagct gggcaagcac
     7921 atggaacacc tggacccctg ccgtgcagat tcccgcggtg tcaggagtga acgccaacgg
     7981 cgatgtctgg actgcatcct ccgcttatag tggtctcgag tacttctccg cgcgcaccgt
     8041 cactgcgagt ggtgtcgtgc cgtaccaatc cgcgaccggc attgcggcgt cgagggcggc
     8101 ggatctcctc acctccggaa agttcgacta tctggaccga tgggccattc ccgcgagcga
     8161 ggccggcgac ggccagagca tcgcggtgga tgaagggggc acgtcgttgt ggcttgccgc
     8221 gtcgtctgct tcgggcacgc ggccctacgt gtacgacttt cccgactgcc ggtcgggaag
     8281 cccgggcgac accaactgcg cgcgctggat gccgggaaac tcgggaaatg actacgtgga
     8341 tctcctctca gatgggggcg atgatgaggt cggcatgagc gcgtactcgc acaagaccgt
     8401 ggtcgtgaac ccctgcacgc atcacgctct cgtcagctac ctggagacca acggcaccgt
     8461 gacgaacatg aaggtcaagg ccgtcagccg caccggcgcg atcactggac ggtggacgtt
     8521 tccaacgtca cagcagccca atgaccgctg cacggggacg acgacgttca cctgcagcgg
     8581 aggtaacagg tacctctgcc cgcctcctgc ctccggagcg gataactgcg gctacaatcg
     8641 ctatgtcccg cgcgtccagc ttgcgacgaa agttgtagcg gaaacctcgc aatgcctgct
     8701 ctatgcaggc tgggatgagt cctacacttc gagcgtgggg cagcggctgc gagccgtgat
     8761 ggcaatcatc gacgtgactg cttcaccgag caagcctgag gcccccgcga gagtcatcca
     8821 tagcactcct cccgacggta cgcagtacta tggctccacg ccagtcatat cgcgctacgg
     8881 cgatagcatt gggtggtttt ttgtcgccac ggccccgccg tacgacaata accacatcct
     8941 ggggtatgtc tcgccggtgc acaacatgga cctgatgact tcgaccgtcg tcttcgtgtc
     9001 cgatacgttc gccaacacct ggatgggaga caatatgtcg cagctgctcg gcggtcttcc
     9061 cggtggcgac ctgtttgcga catggccgga gtctatcaac ggctgctcgc agatagcggg
     9121 agccagggtc acccccccat acagtcccag tgcgtgccag gcggcgagcg aacagtacgg
     9181 catcgtggcc ggcagtacgt ttggctacgc gccccccgac gtccggtcgt ggtggatcgg
     9241 cgccggctgc accacctcac ctctctacac ccagaatctc tgccagcgcg ccagcgacgt
     9301 ctacggcatc atcgccaacc agaccttcgg cacggcacct gcatccgtcc gagattggtg
     9361 gacatccaat ggatgcaaca cgacccccac cccgaagaat acctgcgagc gcgcgagcca
     9421 gttgtacgga atcactaaca atgtctcctg gggattcgct ccgagctctg tgcagacatg
     9481 gtggactgcg aacaactgca acgctcagca gaccgtaccg gtggcggcat gccagcgcgc
     9541 cgctgatctg tacgggatcg tcgctaacga gacgtgggga ttcgctcccg acgatatcaa
     9601 ggactggtgg cgcacgcatt cgtgcaacgc gcgcgcgcaa tcgaccaacg catgccagcg
     9661 cgcgagcgaa cgctacggca tcattgcaaa caagacgttc ggcttcgcgc ccgcgagtgt
     9721 ccagacgtgg tggatcagca acggatgcaa tgacgtgcct ctcaagacat cagacgcgtg
     9781 ccaagttgcg agcaacctgt tcggaattga cgcgggggtg tccttcgcct tcgcgcccga
     9841 tgatgtccag gcctggtgga aaggggctgg ttgcaacgcg catccgcaat gatcggagcg
     9901 cgcatccggg tgatgccttg tcgagcgacc tcctgctttc gcgcatcgag aagctcacgg
     9961 ctggaggtca tggagatcgg gccagcccct actccagctc gacgccagtc gcgaccagga
    10021 accgcgaggc catgttgtag cccgcgatcg tcccgatcag ctcgatcacc tggtcgagcg
    10081 gcaacacggc gcggatccgc ttcatcgtcg cgtcggacac cttgatcgag cgcgtcatct
    10141 cgatggtgag cgccagcgtc gcgcgctcgg gctcgtcgaa cagcttgtcg ttcttcatcg
    10201 cggccggggg attcttgagc gccgcgagct gcgccgccgt gccgccggcc ttggtgaact
    10261 cggcctcgtg ctgcgcccac tcgtagtcgg cctggttgag cgcgccgacc gccatgatcg
    10321 tcagctcgcg cagcttgggc ggcaccgtga gctg
//
LOCUS       DASVOR010001308         1916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_247419,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001308 DASVOR010000000
VERSION     DASVOR010001308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1916
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247419"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1103
                     /locus_tag="VF516_24980"
     CDS             <1..1103
                     /locus_tag="VF516_24980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111019.1"
                     /translation="RMAANKPPTSLSALNPQITAFINGAMRVDDHPVLTPKGVAIDDR
                     PFLRTAELDLRAAVDPYADAVTILSLENEAGEGFGVDAEEAYVVVKRLPVLESAPLGL
                     KLKLGRFRAPIGSGNRIHMHDLPWTTRPLPIARMLGTENGEFFESGFNPVGAEAEVIL
                     PTVIPGVVQELTADVVDGGDIAIADGRHDHPGFIGHYNLFFTVHDAHDFNFGASGYYQ
                     GGAQRAGLWAVDALYKWKPLAAGEFHSVVIGGELFGADRRFQVATPGGSPVLQRATPL
                     AGYGFAQVQLDWHTYLGARYDYAQDITDPHLATQVAAGYLSYYTSEFLRFRAGYEHRW
                     SDVAADNGTHSFIAEVNVVFGSHPTEPYWVNR"
     gene            1124..>1916
                     /locus_tag="VF516_24985"
     CDS             1124..>1916
                     /locus_tag="VF516_24985"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX8111020.1"
                     /translation="MIPSPILTTIVAALALGLTAGSAAPAAPAKKVSVVTTLNVLAGV
                     TREVGGDRVDVTSLAKPNQDPHTLVAKPTFKVLAKNAHLFVELGLGLDIWGSAVTDAS
                     GNPDIQTGQKGRVIASEGIETKELPATLSKAWGDIHPYGNPHVWLDPVNVKQIAANIA
                     AGLTRVDPGGKDTYAANLKAFSARIDDALYGHALVEEYGASKLERLSRRNELIDYLKA
                     KGTYGKLGGWLKQAEALRGLKIVTYHKTWVYFCDRFGIQIRGEIEE"
BASE COUNT          311 a          704 c          636 g          265 t
ORIGIN      
        1 cgcggatggc ggcgaacaag ccgccgacgt cgctgagcgc gctcaacccg cagatcacgg
       61 cgttcatcaa cggcgcgatg cgggtcgacg accacccggt gctcaccccc aagggcgtgg
      121 cgatcgacga ccggccgttc ctgcgcaccg ccgagctcga cctccgcgcc gcggtcgacc
      181 cctacgccga cgcggtgacg atcctgtcgc tggagaacga ggccggcgag ggcttcgggg
      241 tcgacgccga ggaggcctac gtggtggtca agcggctgcc ggtgctggag tcggcgccgc
      301 tcggcctcaa gctcaagctc gggcggtttc gcgcgccgat cggcagcggc aaccggatcc
      361 acatgcacga cctgccgtgg acgacgcggc cgctgccgat cgcgcggatg ctcggcaccg
      421 agaacggcga gttcttcgag tccgggttca acccggtcgg cgccgaggcc gaggtgatcc
      481 tgccgaccgt gatcccgggc gtggtgcagg agctgaccgc cgatgtcgtc gacggcggcg
      541 acatcgcgat cgccgacggc cggcacgacc acccgggctt catcggccac tacaacctgt
      601 tcttcaccgt gcacgacgcc cacgacttca acttcggggc atcgggctac taccagggcg
      661 gcgcccagcg cgccgggctg tgggcggtcg acgcgctgta caagtggaag ccgctggccg
      721 ccggcgagtt ccacagcgtg gtgatcggcg gcgagctgtt cggcgccgac cgccggttcc
      781 aggtcgccac gcccggcggc tcgccggtgc tgcagcgcgc gacgccgctg gccggctacg
      841 gcttcgcgca ggtccagctc gactggcaca cgtacctcgg cgcccgctac gactacgcgc
      901 aggacatcac cgacccgcac ctggccaccc aggtcgccgc cggctacctc agctactaca
      961 cgtcggagtt cctgcggttc cgcgccggct acgagcaccg ctggagcgac gtcgcggcgg
     1021 acaacggcac ccactcgttc atcgccgagg tcaacgtcgt cttcggctct caccccactg
     1081 aaccctactg ggtcaatcgc tgattcggag cactgcgatg cgcatgatcc cttccccgat
     1141 tctcaccacc atcgtcgccg cgctcgcgct cgggctgacc gcgggcagcg ccgcgccggc
     1201 cgcgccggcg aagaaggtct cggtcgtcac cacgctcaac gtcctcgccg gggtcacccg
     1261 cgaggtcggc ggcgatcggg tcgacgtcac ctcgctggcc aagcccaacc aggaccccca
     1321 caccctggtc gccaagccga cgttcaaggt gctcgccaag aacgcccacc tgttcgtcga
     1381 gctcggcctc gggctcgaca tctggggctc ggcggtcacc gatgcgtcgg gcaaccccga
     1441 catccagacc ggccagaagg gccgggtgat cgcgtccgag ggcatcgaga ccaaggagct
     1501 gccggcgacg ctgtccaagg cgtggggcga catccacccc tacggcaacc cgcacgtctg
     1561 gctcgatccg gtcaacgtga agcagatcgc cgccaacatc gcggccggtc tgacccgggt
     1621 cgaccccggc ggcaaggaca cctacgccgc caacctcaag gcgttcagcg cccggatcga
     1681 cgacgcgctg tacggccacg cgctggtcga ggagtacggc gcgagcaagc tcgagcggct
     1741 gtcgcggcgc aacgagctga tcgactacct caaggccaag ggcacctacg gcaagctcgg
     1801 cggctggctc aagcaggcgg aggccctgcg cgggctcaag atcgtcacct accacaagac
     1861 ctgggtgtac ttctgcgatc ggttcggcat ccagatccgc ggcgagatcg aggaga
//
LOCUS       DASVOR010001309         4099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_159323,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001309 DASVOR010000000
VERSION     DASVOR010001309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4099
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159323"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF516_24990"
     CDS             complement(<1..617)
                     /locus_tag="VF516_24990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEX8111021.1"
                     /translation="MNIIEAVRSALQTQMRADPRVVVLGEDVGKFGGVFRATSGLYDE
                     FGGDRVIDTPLAEAGIIGTAIGMALYGLRPVPEIQFGDFIYPAFDQIVNELAKFRYRS
                     GGQYPCPVVIRTPVGGGIRGGHYHSQSPEALFIHTPGLKVVAPSNPYDAKGLLLAAMR
                     QNDPVLFMEPKRIYRASRGDVPEGEYTVELGEAAIVRAGTQVTVVA"
     gene            complement(648..1724)
                     /locus_tag="VF516_24995"
     CDS             complement(648..1724)
                     /locus_tag="VF516_24995"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX8111022.1"
                     /translation="MLDGGGKPIPGARVPSIPDATLARMFEVMLQSQILMRVAHTVQL
                     GGQLVAMGEEAAQLAVAPLEAGDWVFSGQGDHVAWLWRGYTIQQLVDQTLGNADDPSK
                     GRQAPAHHSARWLNLVSISSPAGTQLPQAVGAAHAARMLGRDDVTMALFGEAATSTGE
                     FHVGMNFAAVWKAPCVFVCRNSGWAGRAQAVQTAARTIASKAIGYGMPGVRVDGNDPL
                     AVWQAASEAIARARAGDGPTLIEALTHVLFRAPEPPDGNSWLESPPAGLELREEPSPK
                     DPLHRLRGYLRHLGLWSERWEAERAERCFDEITRAVAAAEDKPPPPVETLFDDVYEHL
                     PWHLRDQRAYLQGLPRAVAGASQK"
     gene            1935..2561
                     /locus_tag="VF516_25000"
     CDS             1935..2561
                     /locus_tag="VF516_25000"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEX8111023.1"
                     /translation="MIWILLVLLTVIGGAAAVGIAAGERRKSLPGPSPSPLGLPAGDK
                     LVERGVAELRVDDVLTIDGKDFLVEGMINYDEDGHRWIGARVVDGSDVKWLVVGIERA
                     GTGAMRLLVQDESTQISGYPPEVLVIGDVRYTLDKRGAATCALHGDLGALGALQKDRP
                     EGHVERARWWLYAAPGDDTLLLEQWGTDYRVLRGKKIGDGTIELIPGS"
     gene            complement(2737..3882)
                     /locus_tag="VF516_25005"
     CDS             complement(2737..3882)
                     /locus_tag="VF516_25005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111024.1"
                     /translation="MFQNVIEAMVDAVLVVDVEGAVTLTNTAAARLTGYSVDELRGMP
                     IAQLLSDERSGLRTVVRRRIEDGAVLRREESWLVPKDGPPIPVSVTGSPVMSDNGLQG
                     IVLVARDVREIRELLGAKESEIARRRRAEDELRAAKASIEEQLDETRKSLLLAERRAT
                     LGTLAGGVGHELRNIAQIQVAAVDELLGALAADEDVTALARTLLPDLERVAEHIAEHG
                     KRLMQLARPGPDHVGPIDLDAVIKDVIAMLRGAGKLRRLEVITEVGPEPLIVTVNRTR
                     IEQILVNLVVNAVDAIGDHTGTITVEVHPSADGKRLVCEVRDTGCGISPENLTRIFQP
                     FFTTKSEDKGTGLGLPVARGIVESYGGTLSATSEVGVGTTFTFDLPR"
BASE COUNT          553 a         1437 c         1503 g          606 t
ORIGIN      
        1 gccacgaccg tgacctgcgt gccggcgcgg acgatcgccg cctcgccgag ctcgacggtg
       61 tactcgccct cgggcacgtc gccccggctc gcgcggtaga tccgcttggg ctccatgaac
      121 aggacgggat cgttctgccg catcgccgcc agcagcaggc ccttggcgtc gtacgggttg
      181 ctcggcgcga cgaccttgag gccgggcgtg tggatgaaca gcgcctcggg cgattgcgag
      241 tggtagtggc cgccgcggat cccgccgccg accggcgtgc ggatcaccac cgggcagggg
      301 tactgcccgc cggaccggta gcggaacttc gccagctcgt tcacgatctg gtcgaacgcc
      361 gggtagatga agtcgccgaa ctggatctcg ggcaccgggc gcaggccgta cagcgccatg
      421 ccgatcgcgg tgccgatgat gccggcctcg gcgagcggcg tgtcgatcac gcggtcgccg
      481 ccgaactcgt cgtacaggcc gctggtggcc cggaacacgc cgccgaactt gccgacgtcc
      541 tcgccgagca cgaccacgcg cgggtccgcg cgcatctggg tctgcagcgc gctgcggacg
      601 gcttcgatga tgttcatggt gggcatggcg gcgatctcta cttcacttca cttctggctg
      661 gctccggcga ccgcgcgcgg cagcccctgc aggtaggcgc gctggtcgcg caggtgccac
      721 ggcaggtgct cgtagacgtc gtcgaacagg gtctcgaccg gcggcggcgg cttgtcctcc
      781 gcggcggcga ctgcacgggt gatctcgtcg aagcagcgct cggcgcgctc ggcctcccag
      841 cgctcgctcc acagcccgag gtggcgcagg tagccgcgca ggcggtgcag cggatccttc
      901 ggggacggtt cttcccggag ctcgaggccc gcggggggcg actcgagcca cgagttgccg
      961 tcgggtggct ccggagcccg gaacagcacg tgggtcagcg cctcgatcag cgtcgggccg
     1021 tcaccggcgc gggcgcgcgc gatcgcctcg ctcgcggcct gccacaccgc gagcggatcg
     1081 ttgccgtcga cccggacgcc cggcatgccg tagccgatcg ccttgctcgc gatcgtccgc
     1141 gccgcggtct gcacggcctg cgcgcggcct gcccagccgc tgttgcggca cacgaacacg
     1201 cacggcgcct tccacaccgc ggcgaagttc atgccgacgt ggaactcgcc ggtcgaggtc
     1261 gcggcctcgc cgaacagcgc catcgtcaca tcgtcgcgcc ccagcatgcg ggccgcgtgg
     1321 gcggcgccga ccgcctgcgg gagctgggtc ccggccggcg agctgatcga caccaggttc
     1381 agccagcgcg ctgaatggtg cgccggagcc tggcggccct tgctcggatc gtccgcgttg
     1441 ccgagcgtct ggtcgacgag ctgctggatg gtgtagccgc gccacagcca cgcgacatgg
     1501 tcgccctggc ccgagaacac ccagtcgccg gcctcgagcg gcgcgaccgc gagctgggcg
     1561 gcctcctcgc ccatcgcgac cagctgaccg ccgagctgga cggtgtgcgc gacccgcatc
     1621 aggatctggc tctgcagcat cacctcgaac atccgggcga gcgtcgcgtc ggggatgctc
     1681 ggaacgcggg cgccggggat cggcttgccg ccgccgtcga gcacgtgacg cacgtctttt
     1741 tcactcgccg gagcagcgac catggatcct cgcaggcgta ggagcgggtc cggtttacgt
     1801 gtgcgccgac gacgtgtcaa gcaggccggc tcgccatggc gcccgcgtgc cgccgcgtgc
     1861 cgccccggcg gtgcgtcacc attgtgacgc gcgatgcgtt gcgttgacgc gctgcgtgcg
     1921 tgaatagttg cccggtgatc tggatcctgc tcgtgctgct caccgtgatc ggcggcgccg
     1981 cggcggttgg catcgcggcg ggcgagcgcc gcaagagcct gccgggcccg tcgccgtcgc
     2041 cgctcgggct gcccgccggc gacaagctgg tcgagcgcgg cgtggccgag ctccgggtcg
     2101 acgacgtgct gacgatcgac ggcaaggact tcctggtcga gggcatgatc aactacgacg
     2161 aggacggcca ccgctggatc ggcgcgcgcg tggtcgacgg cagcgacgtc aagtggctgg
     2221 tcgtcgggat cgagcgcgcc ggcaccggcg cgatgcggct gctcgtgcag gacgagtcga
     2281 cgcagatctc gggctaccca cccgaggtgc tggtgatcgg cgacgtgcgc tacacgctcg
     2341 acaagcgcgg cgccgcgacc tgcgcgctgc acggcgacct cggcgcgctc ggcgcgctcc
     2401 agaaggaccg cccggagggc cacgtcgagc gcgcgcggtg gtggctgtac gccgcgcccg
     2461 gcgacgacac cctccttctg gagcagtggg gaaccgacta ccgcgtgctg cggggcaaga
     2521 agatcgggga cgggacgatc gagctgatcc cggggagttg atgcctcccg cagtggacct
     2581 gcaggtgcag agctgatccc gggaagtgaa tgcaacccgc agtggacctg caggtgcagg
     2641 gccgcgcgac gcgacgcgcg gaggggtgaa tggaccggct ttgccggtcc atcccccaga
     2701 ggggggtcat gggaaagccc cctctcgatc gaccggtcag cgcggcagat cgaacgtgaa
     2761 cgtcgtcccg acgccgacct cggaggtcgc gctcagcgtg ccgccgtagc tctcgacgat
     2821 gccgcgcgcg accggcaggc cgaggccggt gcccttgtcc tcggacttgg tcgtgaagaa
     2881 cggctggaag atccgggtga ggttctccgg cgagatcccg cagccggtgt cgcggacctc
     2941 gcagaccagg cgcttgccgt cggcgctggg atgaacttcg acggtgatcg tgccggtgtg
     3001 gtcgccgatc gcgtcgaccg cgttgaccac caggttgacc aggatctgct cgatccgcgt
     3061 gcggttgacc gtgacgatca gcggctcggg gccgacctcg gtgatcacct cgagccggcg
     3121 cagcttgccc gcgccgcgca gcatcgcgat cacgtccttg atcacggcgt cgagatcgat
     3181 cgggccgacg tggtcggggc cgggccgcgc gagctgcatc agccgcttgc cgtgctcggc
     3241 gatgtgctcg gcgacgcgct cgaggtcggg cagcagcgtg cgggcgagcg cggtgacgtc
     3301 ctcgtcggcg gccagcgccc cgagcagctc gtcgaccgcg gcgacctgga tctgggcgat
     3361 gttgcgcagc tcgtggccga cgccgccggc cagcgtgccg agcgtggcgc ggcgctcggc
     3421 gagcagcagc gacttgcggg tctcgtcgag ctgctcctcg atcgacgcct tggcggcgcg
     3481 cagctcgtcc tcggcccgcc gccgccgcgc gatctcggac tccttggcgc cgagcagctc
     3541 gcggatctcg cggacatcgc gagccaccag cacgatccct tgtaggccat tatccgacat
     3601 cacgggggag ccggtgaccg agaccgggat cggcgggccg tccttgggga cgagccacga
     3661 ctcctctcgg cgcagcacgg cgccgtcctc gatccggcgg cggacgacgg tgcgcagccc
     3721 ggaccgctcg tcgctgagca gctgcgcgat cggcatcccg cgcagctcgt cgaccgaata
     3781 gccggtgagc cgcgcggccg ccgtgttggt cagcgtcact gcgccttcca cgtcgacaac
     3841 caacacggca tcgaccatcg cctcgatgac gttctggaac acctgcgctc gaacagtatc
     3901 atgactgcgc gagcgactgg cccgaccagc tgtagagtcg gtatgtggcc cggtgctcga
     3961 catgtcaccg ccgactggca gcggggcgcg cctgtcccgc ccacggggga gcgccggcgc
     4021 cggcagctcg gatcgacgcc ggcgtccgcc cggtgtggtc ggcgccggtc ggtgcgtgca
     4081 tcgggagcgg cgggttcgc
//
LOCUS       DASVOR010001310         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_481212,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001310 DASVOR010000000
VERSION     DASVOR010001310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481212"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="VF516_25010"
     CDS             <1..673
                     /locus_tag="VF516_25010"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8111025.1"
                     /translation="DPPAPESRGTLEIDSTPGGAIITLGDHRLGPAPRHERVAGGVKV
                     HVKLELQGYQPYEDDLAVEPGKTLMFRPKLTAAPAVLHVETTPAGAAVTLAGQLQGTS
                     PLTVPIAAARGVELALSRAGYEPLKLKVDLAAGEETRVARELRELQKFGTVLVLVGGA
                     ADWGYVWYKGKNLGQNYTMAGGQTPFKLPVGRQQLRIEHPQAPPKTVTVDVVEHEPTR
                     ITVTL"
     gene            complement(683..976)
                     /locus_tag="VF516_25015"
     CDS             complement(683..976)
                     /locus_tag="VF516_25015"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEX8111026.1"
                     /translation="MTIQGDGEPVAVDVPAGTTLFEAGAKLSAGIDTACVGKGTCGLC
                     RVKIVAGAEHLSPYSDEERKHLGNVYHLTRVRLACRSQLTGGDVAIRIVRRKK"
     gene            complement(1042..1668)
                     /locus_tag="VF516_25020"
     CDS             complement(1042..1668)
                     /locus_tag="VF516_25020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111027.1"
                     /translation="MCAAALIAATSRVASAQPVDITVIPPGCEQYAAGVIPDPDQAWN
                     QLMSFAGCVQDATIARITDRAQLRPVVDQLHDALTPAIALYLEAIEYGPPEIRLRAAY
                     ATGSANIALITRLRRSLVAPADWSDGAAVARYRALQAELEPLLVRAQRTAWVAFSVVD
                     RAAARDPSIARDPVARYAVTAARAMLAQMKRVRELTGPAPVVGARTAI"
BASE COUNT          259 a          701 c          659 g          227 t
ORIGIN      
        1 cgaccctccc gcccccgagt cgcgcggaac gctggagatc gactcgaccc ccggcggcgc
       61 gatcatcacg ctcggagatc accggctcgg accggcgccg cgccacgagc gcgtggcggg
      121 cggcgtgaag gtccacgtca agctcgagct ccagggctac cagccgtacg aggatgacct
      181 cgcggtcgag cccggcaaga ccctcatgtt ccgccccaag ctgaccgcgg cgcccgcggt
      241 gctgcacgtc gagaccacgc cggcgggtgc ggcggtcacg ctcgccgggc agctccaggg
      301 gacctcgccg ctgaccgtgc cgatcgccgc cgcgcgcggc gtcgagctcg cgctgtcccg
      361 ggcgggctac gagccgctca agctcaaggt cgacctggca gccggcgagg agacccgggt
      421 ggcccgcgag ctgcgcgagc tgcagaagtt cggcaccgtg ctggtcctgg tcggcggcgc
      481 cgccgactgg ggctacgtct ggtacaaggg caagaacctc ggccagaact acacgatggc
      541 cggcggccag accccgttca agctgccggt cgggcgccag cagctgcgga tcgagcaccc
      601 gcaggcgccg ccgaagacgg tgacggtcga cgtcgtcgag cacgagccga cgcggatcac
      661 ggtcacgctg tagccgggct cgctacttct tgcggcggac gatccggatc gcgacgtcgc
      721 cgccggtcag ctgcgaccgg cacgccagcc ggacccgggt gaggtggtag acgttgccca
      781 ggtgcttgcg ctcctcgtcg ctgtacggcg aaaggtgctc ggcgccggcg acgatcttga
      841 cccggcatag cccgcacgtg cccttgccga cgcacgcggt gtcgatcccg gccgagagct
      901 tcgcgccggc ctcgaacagc gtcgtcccgg ccggaacgtc aacggcgacc ggctcgccgt
      961 cgccctggat ggtcacccgc ggcagagccc gcatcctacc cggcccgcgg cgctccagcg
     1021 agcggtcgat cgaccgctcg ctcagatcgc agtccgcgcc ccgaccaccg gcgccggccc
     1081 ggtgagctcg cgcacccgct tcatctgcgc gagcatcgcg cgcgccgcgg tgaccgcata
     1141 gcgcgccacc ggatcgcggg cgatggatgg atctcgcgcc gccgcgcggt cgaccaccga
     1201 gaacgcgacc cacgcggtgc gctgggcgcg gaccagcagc ggctccagct cggcctgcaa
     1261 cgcgcggtag cgcgcgaccg ccgcgccgtc cgaccagtcc gccggcgcga ccagcgagcg
     1321 gcgcagccgg gtgatcagcg cgatgttcgc gctcccggtc gcgtaggcgg cgcgcagccg
     1381 gatctccggc gggccgtact cgatcgcctc gaggtagagc gcgatcgccg gcgtcagcgc
     1441 atcatggagc tgatcgacca ccggccggag ctgcgcccgg tcggtgatgc gcgcgatcgt
     1501 cgcgtcctgc acgcagccgg cgaacgacat cagctggttc cacgcctggt ccggatccgg
     1561 gatcacgccg gccgcgtact gctcgcaccc gggcgggatc acggtgatat ccaccggctg
     1621 cgccgaggcg acgcgcgagg tcgccgcgat cagcgcggcg gcgcacacga atcggagccg
     1681 tttccctcga gcttgctcgg ccatgcccga tcgcggtgca aggccgacgc cacaccgggc
     1741 gcgcgggcac ggtggtgtgc cgatcgccgt catcggcccg cacgatctgc gcggcgcgac
     1801 gcccgtgcgc cgaacgcgcc gcgcgcccgc gaccgatcgt cggatc
//
LOCUS       DASVOR010001311         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_14253,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001311 DASVOR010000000
VERSION     DASVOR010001311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14253"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF516_25025"
     CDS             complement(<1..216)
                     /locus_tag="VF516_25025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111028.1"
                     /translation="MTSVGHHPLVGQLLRSVGASENEAPSLETWRELLARISQMYHET
                     DQDRDELESSIESSSREMQRLYQELKQR"
     gene            558..631
                     /locus_tag="VF516_25030"
     tRNA            558..631
                     /locus_tag="VF516_25030"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:592..594,aa:Met,seq:cat)
     gene            965..1249
                     /locus_tag="VF516_25035"
     CDS             965..1249
                     /locus_tag="VF516_25035"
                     /inference="COORDINATES: protein motif:HMM:NF039950.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5984 family protein"
                     /protein_id="HEX8111029.1"
                     /translation="MQRFRDELVRFDQDLIAAMQARVDALATGRPRPDIAIDVDALHR
                     EQADRAIWLRCAGSASSRAFVVEHDAAGAAVARPHRRHQLGPARCASRSL"
     gene            1561..1917
                     /locus_tag="VF516_25040"
     CDS             1561..1917
                     /locus_tag="VF516_25040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111030.1"
                     /translation="MDQQTVTLVAAGVAATASLSNVIVNVYFARRMSRESQFRDLLKP
                     HMVVLGETLHAVVACSRVVGARAQQGIDTRSWLKKAQDACNQLKQLHRQVKYPLWGVS
                     EGIRELTRLRRDRHGR"
BASE COUNT          318 a          700 c          628 g          348 t
ORIGIN      
        1 ccgctgcttc agctcctgat acagccgctg catctcgcgc gacgaactct cgatcgagct
       61 ctccagctca tcgcgatcct ggtcggtctc gtgatacatc tggctgatcc gggccagcag
      121 ctcgcgccag gtctccagcg aaggcgcctc gttctcgctc gcgcccacgc tgcgcagcag
      181 ctggccgaca aggggatgat gcccgacgga ggtcatgatg gagcggaacg atacaggccc
      241 ggcggtcgta cagcctcgtt atccaactac accatggatg gcccgcccaa cgtcgtgctc
      301 gcagtcgaag tcatagccga cagtgttcgg atgcttgcac atcatcggcc attgtctgat
      361 gcctcgccgt ccgccgtgtt cgtcctcgct gcagcgattt actgcagtat ccgcgcttac
      421 ttgctactca cgctccgatg ctcccggccg ccagcgcgcc gatgcacggg ccaccgccgc
      481 aggctgccgc gaagtgcagc cccaggcctt gacgcctggt cccccggggg ctagaaacac
      541 tcgcggtgac aacttgatgc ggggtggagc agcctggtag ctcgtcgggc tcataacccg
      601 aaggtcgtag gttcaaatcc tacccccgct actcgaatga aatcgcggag ttaggcctca
      661 aaccctatct ccgcgtttcg cttttcggcg ccgccgtcgc tgatcacgat cagcggcagg
      721 agtttcccct cgtccacgat ggcgccgtcc ttgttcggcc gatcggatgg agccgtcctt
      781 gagccagcgg cgcagctgct cgcggccggc ctcctcctga ccccggtcgc cgccggcggc
      841 gagctggtgg acctgaccga gtccgacctg cgacgtcgcc caaaggagct gctcaccggg
      901 ctctccggac ggtggcagtg tcggcggcta cgacctcgat gttgagctgg acgcgatcgt
      961 ctccgtgcag cgcttccggg acgagctggt gcgcttcgat caggacctca tcgctgcaat
     1021 gcaggcccgc gtcgacgcgc tggccaccgg acggccccgt cccgacatcg cgatcgacgt
     1081 ggatgccttg caccgtgagc aggccgaccg cgcgatctgg ctccgctgcg cgggcagcgc
     1141 gagcagccgg gcattcgtcg tcgagcacga tgccgccggt gcggccgtgg ctcgaccgca
     1201 ccggcgtcac cagctcggtc cggctcgctg cgcatcgcgc tcgctatagc agcatcatcg
     1261 attcgccggg cgccctgaac ggcgcgcgcg tgctttagac ggaccgggct cgccgagttc
     1321 gaaccgcggc ggcgcaccgg tccggggacc gtacttgccc ggccccgggg tgcggcgtag
     1381 agccgcttgc gagcaccggc ctggcccggt gcagcggggc ttcactcccg atcgccgcca
     1441 agctcaccgc gccccagcca ccgaacagcc gctatcggcc cgctgcctct gtcatcggac
     1501 ttgccaccgc gaggggcact gccggttgtg ggcaagaatc gatgctgcga atctcgagca
     1561 atggaccagc agaccgtcac cttggtggcc gccggcgtcg ctgcaacagc ttcattatcc
     1621 aacgtcattg tcaacgtgta tttcgctcgg cggatgtctc gcgagtccca gtttcgagac
     1681 ctgctgaaac cgcacatggt cgtgctcggc gagacgctac acgcggtggt cgcctgcagt
     1741 cgtgtggtcg gggctcgagc acaacaaggc atcgacaccc gctcgtggct caagaaagcc
     1801 caggatgcct gcaaccagct gaagcagctg catcgccagg tcaagtatcc gctctggggg
     1861 gtaagcgaag gtatccgaga gctcacccgg cttcgacgcg accgccatgg ccgctgaagt
     1921 ggaccaccgt cggcatcagc atccccagcc cgtcgagcag gtcgagcggt cgcgcgggct
     1981 ggcaaggact cccg
//
LOCUS       DASVOR010001312         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_510209,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001312 DASVOR010000000
VERSION     DASVOR010001312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510209"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VF516_25045"
     CDS             complement(<1..579)
                     /locus_tag="VF516_25045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX8111031.1"
                     /translation="MRIRAAQLDTEDHWLTAAEGRTAGQIERLVAGHQPGDQPEDPTH
                     PDLRPRVVRLELPPEVYALWRQAHAIIAAERGAEIRDTDFVESLCRAVIAPGTGAAGP
                     AHQIAYQQCPDCRRATQNGAGREIDIAPEVFERAACDARDLGSLDAPAPERATTTVTP
                     RLRAQVFARDHHRCTVPGCRSARNLDIHHIVPQ"
     gene            691..1554
                     /locus_tag="VF516_25050"
     CDS             691..1554
                     /locus_tag="VF516_25050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111032.1"
                     /translation="MPETAQAIQMHDTTPSHIIPSPRYWHLPWSLLGLFAMGVPACTD
                     PPATTEESAAVARGDDDDFPVIPHLPAQPTLVVSTIPPSGDLNPYGVAFVPKGFPRGG
                     KIHPGDILVSNFNNSANIQGTGSTIVTIHPDGKQTVFFQGPPGLGLSTALGVLRGGFV
                     VVGNVPSIHGSGVCTQVDGQETGVGQGALRILDKHGEVVRTITSKQLLDGPWDLTIRD
                     RGERAAVFVSNVLSGTVTRLDLELDDQRCHDGGCVEVERKAQIASGYLHRCDPAAFVS
                     GRAGDRGARAA"
     gene            complement(1514..1750)
                     /locus_tag="VF516_25055"
     CDS             complement(1514..1750)
                     /locus_tag="VF516_25055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX8111033.1"
                     /translation="MAVDPEAARAGDRAALEELLRERRTEVVRYAMRLCISPADAEDA
                     VQETLIDDSVVADRRTRRLRSITPLALRDRQHGR"
     gene            1981..>2465
                     /locus_tag="VF516_25060"
     CDS             1981..>2465
                     /locus_tag="VF516_25060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111034.1"
                     /translation="MSKLPLAALAILAAAAPLAGAAGEAPKKLSLVPGEREPAQIDLP
                     GAAMLVELRQKVVDKPKDREARFNYVRALMRARQLPDALAAAREWRAIDAYNLVVVRL
                     IGDIYSELGDRTAARRAYSAVVELLPKDATAQRALASVLKQAGDLDGAYDRLAAAAEL
                     SP"
BASE COUNT          358 a          866 c          861 g          380 t
ORIGIN      
        1 ctgcggcacg atgtggtgaa tgtccaggtt gcgggctgaa cggcagccgg gcaccgtgca
       61 gcggtgatgg tcgcgcgcga acacctgcgc gcgcagccgc ggcgtcaccg tggtcgtcgc
      121 gcgctccggc gccggcgcat cgagcgagcc gagatccctg gcgtcgcacg cggcccgctc
      181 gaagacctcc ggggcgatgt cgatctcccg gccggcaccg ttctgcgtcg cgcgccggca
      241 gtccggacac tgctggtagg cgatctgatg cgccgggccc gcggcgccgg tgcccggcgc
      301 gatcaccgcg cggcacagtg actccacgaa gtccgtgtcg cggatctccg cgccgcgctc
      361 ggcggcgatg atcgcgtgcg cctgccgcca tagcgcgtag acctccggtg gcagctcgag
      421 ccgcaccacg cgcggccgca ggtcggggtg cgtcggatcc tccggctgat cgccgggctg
      481 gtggcccgcg accaggcgct cgatctgacc ggccgtcctg ccctcggcgg ccgtcagcca
      541 gtggtcctcc gtgtcgagct gcgccgcgcg tatgcgcacg tctgttgttg tggtcgaggt
      601 gaatgccgcc gaatgcgtaa cgagttagtt gcgttgctcg gcgaacgtcc ctgtcagcgc
      661 gtggttcgat cgatgcacgc tgctcgttcc atgccggaaa ccgcacaggc gatccagatg
      721 catgatacca caccatctca cattatcccg agtccccgct actggcattt accatggagc
      781 ttgctcgggc tgttcgcgat gggcgtacct gcgtgtaccg acccgcccgc aacgaccgag
      841 gagtcagcgg ccgtcgcgcg cggtgacgat gacgactttc ccgtgatccc ccatctgcct
      901 gcgcagccca ccctcgtcgt ctcgacgatc cctccgtcgg gcgatctgaa tccatacggc
      961 gtggcgttcg tcccgaaagg cttcccgagg ggcggcaaga tccatccagg cgatatcctg
     1021 gtctcgaact tcaacaacag cgccaacatt cagggaaccg gatcgacgat cgtgacgatc
     1081 catcccgacg gtaagcagac cgtgttcttc caggggccac cggggcttgg tctgtcgacc
     1141 gcgctgggcg tgctccgggg cggattcgtg gtcgtcggga acgtgccgag catccatggc
     1201 agcggggtct gcacccaggt cgacggtcag gagaccggcg tcggtcaagg cgcgctgagg
     1261 attctcgaca agcacggaga ggtcgtccgc acgatcacga gcaagcagct cctggacgga
     1321 ccgtgggatc tgacgattcg cgaccgcgga gaacgcgcag cggtgttcgt atcgaacgtc
     1381 ctgagcggca ccgtgacgcg gctcgatctg gagctcgacg accaacgctg ccatgacggg
     1441 ggctgcgtgg aggtggaacg aaaggcgcag atcgcctctg gctatctcca ccgctgcgac
     1501 ccggccgcgt tcgtcagcgg ccgtgctggc gatcgcggag cgcgagcggc gtgatgctgc
     1561 gcaggcggcg tgttcgccga tcggccacga cgctgtcgtc gatcagggtc tcctggaccg
     1621 cgtcctcggc gtcggcgggc gagatgcaga gccgcatcgc gtagcgcacg acctcggtgc
     1681 gacgctcgcg caacagctcc tcgagcgcgg cccggtcgcc ggcacgcgca gcctccggat
     1741 cgacggccat cgcagcgtgc gcgaacctgg cacggggctg caccgcggcg gccgatcgac
     1801 cgatcgagtc ggtcccggcg gcacgtcgca agcggtttat tcccgccgag attgcacggc
     1861 ggacctcgcg ggacccgtgt ccggcggtcg acgcgggccg tgtcgctttt gcgacagccg
     1921 cggcaacctc gcgcagttgc tcgcgcggca ggtggcacct cggctgcaag ttgcggcggc
     1981 atgtccaagc tccctctggc cgcgctcgcc atcctggcag ccgcggcccc gctggccggc
     2041 gccgccggcg aagccccgaa gaagctgtcg ctcgtgcccg gcgagcgcga gccggcccag
     2101 atcgacctgc ccggtgcggc gatgctcgtc gagctgcgcc agaaggtggt cgacaagccc
     2161 aaggatcgcg aggcgcggtt caactacgtc cgcgcgctga tgcgcgcccg ccagctgccc
     2221 gatgcgctcg cggcggcgcg agagtggcgc gcgatcgacg cctacaacct cgtggtcgtc
     2281 cggctgatcg gcgacatcta cagcgagctc ggcgacagga ccgccgcgcg ccgcgcgtac
     2341 tcggcggtgg tcgagctcct gcccaaggac gccaccgccc agcgcgcgct ggccagcgtg
     2401 ctcaagcaag ccggcgacct cgacggcgcg tacgatcggc tggcggcggc cgccgagctc
     2461 agccc
//
LOCUS       DASVOR010001313         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_247526,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001313 DASVOR010000000
VERSION     DASVOR010001313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247526"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..539
                     /locus_tag="VF516_25065"
     CDS             168..539
                     /locus_tag="VF516_25065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111035.1"
                     /translation="MPSDRAPIHVLAALLGVANLVFGFFAFTHHLPGALTATLLVSGL
                     LVPTLAHFSYRRSRAAWAFLVALCGVFAVVSLFGAPKISHALDVRLGMTMILPALYVV
                     ACVMLALLHANYVDAAPARRV"
     gene            698..1330
                     /locus_tag="VF516_25070"
     CDS             698..1330
                     /locus_tag="VF516_25070"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8111036.1"
                     /translation="MALQLGDDAPDFTAETTEGPLSFHQYLGNSWGVLFSHPKDFTPV
                     CTTELGRVSALKPEFDKRNVKVVGLSVDPLESHRGWVNDIKETQGHALNFPLIADHDK
                     KVANLYGMIHPNASDTFTVRSVFVIGPDKKIKLTLTYPASTGRNFDEILRVIDSLQLT
                     AKHKVATPSDWKQGQDVIIVPSVSDADAKAAFPSGWKTLKPYLRVVPQPK"
BASE COUNT          251 a          612 c          513 g          248 t
ORIGIN      
        1 ccccgagcgc gccgccgccg atcggaccgc cgccgccgga gccggcgatg ctcaacggcg
       61 aaccgccgcc gcccgcggct cccacgccgc cgagcgacaa gccgcgcggg ttctggggca
      121 actggggcaa cttgttcagg aagcgcaagt agcgcatgct cttcgccatg ccgtccgacc
      181 gcgcaccgat ccacgtgctc gccgccctgc tcggcgtcgc caacctcgtg ttcgggttct
      241 tcgcgttcac ccaccacctg ccgggcgcgc tgaccgccac cctgctggtg agcgggctcc
      301 tggtccccac gctggcacac ttctcctatc gccgctcgcg cgccgcgtgg gcattcctcg
      361 ttgcgctgtg cggcgtgttc gcggtcgtca gcctgttcgg cgcgcccaag atcagccacg
      421 ccctcgatgt ccgccttggg atgacgatga tcctccccgc cctctacgtc gtggcctgcg
      481 tgatgctcgc gttgctgcac gccaactacg tcgacgccgc gcccgcccgc cgggtgtgac
      541 gccgcccacc tggccaggtc gccggccgag tgcccggctg ccacggcgag cggtcgcctg
      601 ccctgaccat ggacggcgga ttgatctcgg atccggcttg ccctcgccga gcacgaggac
      661 tagaaccgag ccgagatcac gataccggag gtatgccatg gcattgcagc tcggagacga
      721 cgcaccggac ttcaccgccg agaccacgga gggtccgctc agcttccacc agtatctcgg
      781 caacagctgg ggggttctgt tctcgcatcc caaggacttc acgccggtct gcacgaccga
      841 gcttggtcgg gtctcggcgc tcaagcccga gttcgacaag cgcaacgtca aggtcgtcgg
      901 tctgtcggtc gatccgctcg agtcgcacag gggctgggtc aacgacatca aggagaccca
      961 gggccacgcg ctcaacttcc cgctgatcgc cgaccacgac aagaaggtcg ccaacctcta
     1021 cgggatgatc caccccaacg ccagcgacac cttcaccgtg cgctcggtgt tcgtcatcgg
     1081 cccggacaag aagatcaagc tgaccctgac ctaccccgcg agcaccggcc gcaacttcga
     1141 cgagatcctg cgcgtgatcg attcgctgca gctgaccgcg aagcacaagg tcgcgacccc
     1201 gtcggactgg aagcagggcc aggacgtgat catcgtcccg tcggtgtcgg acgccgacgc
     1261 caaggccgcg ttccccagcg gctggaagac gctcaagccg tacctgcgcg tcgttccgca
     1321 gcccaagtag cgccggcgtc cacgctacgc cggattgcgc atccacggcg gcgccgaccc
     1381 taccgcggcc gccgcgccgc ccgtcagcgc cgatcttcgt gagccggacc tgcgtgtgat
     1441 cgccgcgcag ctatggtggg ttgtccggcc gctctgcgca gatccgcgtg ccgcccgtcg
     1501 cagccggtct cgggccgcat gaggcacggc ggcggcaacc gtggatcgcg gtactcggtc
     1561 aggcggaagc gcgccgcgac cgcaactcgg acgcgagcga gctcatgcgc gaccggggcc
     1621 acca
//
LOCUS       DASVOR010001314         8337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_421965,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001314 DASVOR010000000
VERSION     DASVOR010001314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8337
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421965"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..3531)
                     /locus_tag="VF516_25075"
     CDS             complement(79..3531)
                     /locus_tag="VF516_25075"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8111037.1"
                     /translation="MVTSRIPIQLAILGVLLSPGIAGAQKAKYTRQQDIKIDAKLSER
                     SRPITPKDPKDQSQADQPLSADQVLAVEGLVGSIRAEQEQILIDLITKTPDTEVEEKS
                     DYYFRLGELYAKQQRYWRLEGTRMLIQSDQTRNPQQKAKLKADADANATKAKEYLLQA
                     VKTYKALTDNEAFRNYPKMDMALFYYGFTLQSGKYMKEARSVYDKLLKNYPNSKYVPE
                     AHLAFADYFFEIGQLDDAEARYKMVLKFPKSPAYWYAMYKMGWIDLNKQRFQEALELF
                     YQVAQSTRNDKKNEILNRAAKNDFVRAYAEVGKADKAYVTFQRVDQQYAFKMLEVLAD
                     TYLNQGKSDKAIYVYQELMRQAPTNKNVCEWQHNIALAELSMVGAQPSDKVKEIENLV
                     KLWNGLKGKKTLPAAEAQECHDNAAAMSSEIARAYHSESAKTKNPETLAYADKLYNIY
                     LRVFPDAEDFAETQYYYAELLWSRAEAEKNPRLQTEEWENAALAFTDVVKHGKVSPRL
                     MKESAYAAVLGWKNALNVDPRVKEQADLDKKNDTTAAPQPIPEREQKMLAAFDIYITY
                     VKDPKDDDLVGMKFLKANTYRRYNHYDEAIPMFLDIIEHHKQHETAEFSANLLLDIYN
                     NKKDYRAMLALVDKLDGDPKFLEGKDTLKDVLAKLKVQAVRKDAEKQEQEGKATNDYA
                     KFVACGQRYLDIYNRNPEAADNDQVLYNAGVCFKEGKAISAAILAFNKLEQYYPNAKI
                     TARAVAQLGKAYGDIAFYDRASDKLEQYAKKYAGEKDAYDAMSEAVFFRKGIGDDKQA
                     VDDTKYFIKMFSKTKLQEAANAMFALTGVYEKQGDGDVVIKHLREYIHQFGDRGGADR
                     LVVAYTKIGEVLWHQSCPVKEVDGSCIRVSRERAISGKRQKAGRKGGGEQRRQCGPES
                     KIKLTVVKRDERKLREAMAAFTAAAREFEKRQGKTGGDEAVARHYYGMAKLADADRDF
                     EAYLDLRFPQGLNFDSRPEMKAIAAKSNKRFTDWVTQKQKVGGTAKAKYNAVFAIKDN
                     ADSITAAARIGQISQDFSDALFTAEIPTNVRTGPYAQDATDAYCDRLQEVADPLEATS
                     LEAYGTCLSKSTDLGWFSDWSKLCERELGQIRPEEYPTTSELRTEPNQVAPITDVDLP
                     TRLE"
     gene            complement(3814..5481)
                     /locus_tag="VF516_25080"
     CDS             complement(3814..5481)
                     /locus_tag="VF516_25080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111038.1"
                     /translation="MRPKLSGSLATIVVLGVVAWSGAAGSHLAFAAPKGGGKPAPAPA
                     SGNTRAGDMEFEPDGAAATPPSKTLERAIKLYDKKDFFSASIELKKVIEGESGDDSRN
                     KQRGEFFMGKTLYQMGFYAGSLAYFDRIVKAGDAHTYHGATLKWLAALSRVLPETSGI
                     LAKIGTYDSSKLGDPSLASVHDELLFLLGRHYYRRGGDGDFDKAIALFRQVPRTSEFF
                     IKAKFFEGVTYVRKYEGKPAVDAFKDILIIGEQRPPQYRADDIDNYRELAQLQMARVF
                     YSTQQYDTSIKYFEKLDQNSFDWTESLFEASWAYFMKTLNSKALGNIHTLNAPYFENQ
                     FFPESVLLKAVIYFNYCLYDQAEEAVADFNEKYAPLTKNLTDLVGKYDDNAEFYEYIK
                     KVKAGRAGIDPVTQRLVTSVLNDKTLLKTFAWVDELNHELEMLHKSDKAWQTTQIAAE
                     VNAELTMQQSVAAADAGKVARDRISRLARELGALSRDGSKIKFEILEAKGNRLTAQAA
                     GARVSSDHREEPIIVDDEHFQWKFNGEYWKDELGYYRFKIRSRCPKQ"
     gene            5866..6387
                     /locus_tag="VF516_25085"
     CDS             5866..6387
                     /locus_tag="VF516_25085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111039.1"
                     /translation="MNRRHPVEVLVVYLSISAIAAVAALGVAGCSDNPVGRLCDLGAS
                     TPTVDSVVVASPSLDCVSRTCLHYKPNRPPPPGGNPQPINSGLCTAECSADSDCDRVP
                     ESPCITGFTCAIAVTAGPFCCKKFCICKDYILLPPSGQLEAPAACDATMSINACCNLE
                     GRNNNPSYPLCKS"
     gene            complement(6696..7040)
                     /locus_tag="VF516_25090"
     CDS             complement(6696..7040)
                     /locus_tag="VF516_25090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEX8111040.1"
                     /translation="MNTKQRGDEAERTATELLLATGYEIVECNFRCKAGELDIVARDG
                     DVLVFVEVRSRSDGDHGHAVEMIGRTKQRRVVRVARHYLAIAAPRFERCRFDVVAITA
                     GEPILLKDAFRA"
     gene            complement(7270..7614)
                     /gene="rplS"
                     /locus_tag="VF516_25095"
                     /pseudo
     CDS             complement(7270..7614)
                     /gene="rplS"
                     /locus_tag="VF516_25095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
     gene            complement(7698..>8337)
                     /locus_tag="VF516_25100"
     CDS             complement(7698..>8337)
                     /locus_tag="VF516_25100"
                     /inference="COORDINATES: protein motif:HMM:NF021455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEX8111041.1"
                     /translation="TAQRRPDLFARFRPTAADRKLLPPALHARTHLALVHHPVTDRTG
                     SVVTTSLTNFDVHDLARSSLTYGLAGYHIVTPIAAQRDKAAHIAALWIGDAHGEHRAR
                     ALALVRTAESIEAVIAEIAASHGTAPRVVATSARTESFPGVVRRSPEALRAEAGVDPA
                     PLLILFGTGWGLADPLIPSVSRVLAPIEGTSDWNHLSVRSAGAILLDRLFGR"
BASE COUNT         1165 a         2700 c         2864 g         1608 t
ORIGIN      
        1 cgtggcctcg agaaggtcga tggcgtcgtc atggcgtccg tcatggcatc cgtcctggcg
       61 ttcgtcgggg gcacgcggtt actccagcct cgtcggcagg tcgacatcgg tgatcggcgc
      121 gacctggttg ggctcggtgc gcagctccga ggtggtcggg tactcctccg gccggatctg
      181 gccgagctcg cgctcgcaca gcttggacca gtcgctgaac cagccgaggt cggtcgactt
      241 cgacaggcag gtgccgtacg cctccagcga cgtcgcctcc agcggatcgg cgacctcctg
      301 cagccgatcg cagtacgcgt cggtcgcatc ctgggcgtac gggccggtcc gcacgttggt
      361 cgggatctcg gcggtgaaca gcgcgtccga gaagtcctgc gagatctggc cgatccgggc
      421 cgcggcggtg atcgagtccg cgttgtcctt gatcgcgaac accgcgttgt acttcgcctt
      481 ggccgtgccg ccgaccttct gcttctgcgt gacccagtcg gtgaaccgct tgttggactt
      541 ggccgcgatc gccttcatct cgggccgcga gtcgaagttc aggccctgcg ggaaccggag
      601 gtcgaggtag gcctcgaagt cgcggtcggc gtcggcgagc ttggccatgc cgtagtagtg
      661 gcgtgcgacg gcctcgtcac cgccggtctt gccctggcgc ttctcgaact cgcgggccgc
      721 ggcggtgaac gccgccatgg cctcgcgcag cttgcgctcg tcgcgcttga ccacggtcag
      781 cttgatcttc gactcggggc cgcactgccg gcgctgctcg ccgccgccct tgcggccggc
      841 cttctggcgc ttgccggaga tcgcgcgctc gcgcgatacc cggatgcacg agccgtcgac
      901 ctccttgacc gggcacgact ggtgccacag cacctcgccg atcttggtgt aggcgaccac
      961 caggcgatcg gcgccgcccc ggtcgccgaa ctggtggatg tactcgcgca ggtgcttgat
     1021 caccacgtcg ccgtcgccct gcttctcgta cacgccggtc agcgcgaaca tcgcgttggc
     1081 ggcctcctgg agcttggtct tgctgaacat cttgatgaag tacttggtgt cgtcgaccgc
     1141 ctgcttgtca tcgccgatgc ccttgcggaa gaacaccgcc tcgctcatcg cgtcgtaggc
     1201 gtccttctcg ccggcgtact tcttggcgta ctgctcgagc ttgtcggacg cccggtcgta
     1261 gaacgcgatg tcgccgtagg ccttgccgag ctgggcgacc gcgcgcgccg tgatcttcgc
     1321 gttcgggtag tactgctcga gcttgttgaa cgcgaggatc gccgcgctga tcgccttgcc
     1381 ctccttgaag cacaccccgg cgttgtagag cacctggtcg ttgtcggcgg cctcggggtt
     1441 gcggttgtag atgtcgaggt agcgctggcc gcaggcgacg aacttggcgt agtcgttggt
     1501 cgccttgccc tcctgctcct gcttctcggc gtccttgcgc accgcctgga ccttgagctt
     1561 ggcgagcacg tccttgagcg tgtccttgcc ctcgaggaac ttggggtcgc cgtcgagctt
     1621 gtcgaccagc gcgagcatcg ccctgtagtc cttcttgttg ttgtagatgt cgagcagcag
     1681 gttggccgag aactccgcgg tctcgtgctg cttgtggtgc tcgatgatgt cgaggaacat
     1741 cgggatcgct tcgtcgtagt ggttgtagcg ccggtaggtg ttggccttga ggaacttcat
     1801 cccgaccagg tcgtcgtcct tggggtcctt gacgtaggtg atgtagatgt cgaacgccgc
     1861 gagcatcttc tgctcgcgct ccgggatcgg ctgcggcgcc gcggtggtgt cgttcttctt
     1921 gtcgaggtcg gcctgctcct tgacccgcgg gtcgacgttg agcgcgttct tccagcccag
     1981 caccgcggcg tacgccgact ccttcatcag ccgcggcgac accttgccgt gcttgaccac
     2041 gtcggtgaac gcgagcgccg cgttctccca ctcctcggtc tgcagccgcg ggttcttctc
     2101 ggcctcggcg cgcgaccaca ggagctcggc gtagtagtac tgggtctccg cgaagtcctc
     2161 ggcgtcgggg aacaccctga ggtagatgtt gtagagcttg tcggcgtagg ccagggtctc
     2221 ggggttcttg gtcttggccg actccgagtg gtaggcccgc gcgatctcgc tcgacatcgc
     2281 ggcggcgttg tcgtggcact cctgggcctc ggcggcgggc agcgtcttct tgcccttgag
     2341 gccgttccac agcttcacca ggttctcgat ctccttgacc ttgtcggagg gttgcgcgcc
     2401 gaccatcgac agctcggcga gcgcgatgtt gtgctgccac tcgcacacgt tcttgttggt
     2461 cggcgcctgc cgcatcagct cctggtagac gtagatcgcc ttgtcgctct tgccctggtt
     2521 caggtaggtg tcggcgagga cctcgagcat cttgaacgcg tactgctggt cgacgcgctg
     2581 gaacgtcacg taggccttgt cggccttgcc gacctcggcg taggcgcgca cgaagtcgtt
     2641 cttcgccgcg cggttcagga tctcgttctt cttgtcgttc cgggtcgact gggcgacctg
     2701 atagaacagc tcgagcgcct cctggaaccg ctgcttgttg aggtcgatcc agcccatctt
     2761 gtacatcgcg taccagtagg ccggcgactt ggggaacttg aggaccatct tgtagcgggc
     2821 ctcggcgtcg tcgagctggc cgatctcgaa gaagtagtcg gcgaacgcga ggtgcgcctc
     2881 gggcacgtac ttcgagttcg ggtagttctt gagcagcttg tcgtagacgc tgcgcgcctc
     2941 cttcatgtac ttgccgctct gcagcgtgaa cccgtaatag aacagcgcca tgtccatctt
     3001 cgggtagttg cggaacgcct cgttgtcggt gagcgccttg taggtcttca ccgcctggag
     3061 cagatactcc ttggccttgg tggcgttggc gtcggcgtcg gccttgagct tggccttctg
     3121 ctgcgggttc ctggtctgat cggactggat cagcatccgc gtgccctcga gccgccagta
     3181 gcgctgctgc ttggcgtaca gctcgccgag ccggaagtag tagtcggact tctcctcgac
     3241 ctcggtgtcc ggggtcttgg tgatcaggtc gatcaggatc tgctcctgct cggcgcggat
     3301 cgagccgacc aggccctcga ccgcgagcac ctgatcggcg ctgagcggct ggtcggcctg
     3361 gctctggtcc ttggggtcct tgggggtgat cggccgggag cgctccgaca gcttggcgtc
     3421 gatcttgatg tcctgctggc gcgtgtactt cgccttctgc gccccggcga tgccgggcga
     3481 cagcagcaca ccgaggatcg cgagctggat cgggatacga gacgtgacca tggacagttc
     3541 ccttcttccg gcgggatggg cggcgaggcg gggcggggcg ggtgagccgg gtgagcgaga
     3601 cggcgacgca ggcgaggcga ggccgggtcg gcgggcgaac gggcggccgg aacgacgatc
     3661 gagacgatga gcagacggcg tccgcgccga aacgacgtcc agcccggctt cccccaagaa
     3721 cggggatgcc aggccgagga caggcccaca gaggggttgg ttgcttcgtt cagccgtcat
     3781 gacccgggcg aggcgatggg ccggctgagg aggctattgc ttgggacagc gagaccggat
     3841 cttgaaccgg tagtaaccga gctcgtcctt ccagtactcg ccattaaact tccactgaaa
     3901 gtgctcgtcg tcaacgataa ttggttcttc cctgtgatcg gaggacaccc gggccccggc
     3961 ggcctgcgca gtcaggcgat ttcccttggc ctccaggatc tcgaacttga tcttggagcc
     4021 gtcgcgtgac agcgccccga gctcgcgggc gagccgggag atccggtcgc gggcgacctt
     4081 gccggcgtcc gcggcggcga ccgactgctg catggtgagc tcggcgttca cctcggcggc
     4141 gatctgggtg gtctgccacg ccttgtcgga cttgtgcagc atctccagct cgtggttgag
     4201 ctcgtcgacc cacgcgaacg tcttgagcag cgtcttgtcg ttgagcacgc tggtcaccag
     4261 ccgctgggtc accgggtcga tcccggcccg gcccgccttg accttcttga tgtactcgta
     4321 gaactcggcg ttgtcgtcgt acttgccgac caggtcggtc aggttcttgg tcagcggcgc
     4381 gtacttctcg ttgaagtcgg cgaccgcttc ctcggcctga tcgtagaggc agtagttgaa
     4441 gtagatgacc gccttgagca gcaccgactc ggggaagaac tggttctcga agtacggcgc
     4501 gttgagcgtg tggatgttgc cgagcgcctt ggagttgagc gtcttcatga agtacgccca
     4561 cgacgcctcg aacagcgact cggtccagtc gaacgagttc tggtcgagct tctcgaagta
     4621 cttgatcgac gtgtcgtact gctgcgtcga gtagaacacc cgcgccatct ggagctgggc
     4681 gagctcgcgg tagttgtcga tgtcgtcggc gcggtactgc ggggggcgct gctcgccgat
     4741 gatgaggatg tccttgaacg cgtcgaccgc cggcttgccc tcgtacttgc ggacgtaggt
     4801 cacgccctcg aagaacttgg ccttgatgaa gaactcgctg gtccgcggca cctggcgaaa
     4861 cagcgcgatc gccttgtcga agtcaccgtc gccgccgcgc cggtagtaat gccggccgag
     4921 caggaacagg agctcgtcgt gcaccgacgc cagcgacgga tcgccgagct tggagctgtc
     4981 gtacgtgccg atcttggcca ggatgcccga ggtctcgggc agcacgcgcg acagcgcggc
     5041 gagccacttg agcgtggcgc catggtaggt gtgcgcgtcg ccggccttga cgatccggtc
     5101 gaagtacgcg agcgagccgg cgtagaaccc catctggtac agggtcttgc ccatgaagaa
     5161 ctcgccgcgc tgcttgttcc tggagtcgtc gccgctctcg ccctcgatca ccttcttgag
     5221 ctcgatcgag gccgagaaga agtccttctt gtcgtagagc ttgatcgcgc gctcgagcgt
     5281 cttcgacggc ggcgtcgcgg ccgcgccgtc gggctcgaac tccatgtcgc cggcccgggt
     5341 attccccgat gcgggggccg gcgccggctt gccgccgccc ttgggcgccg cgaacgcgag
     5401 gtgcgatccg gctgccccgg accacgcgac caccccgagc acgacgatcg tcgcgagcga
     5461 accagacagc ttcgggcgca tccattcctc cttcgggcgg tgcccggctc cggacggagc
     5521 gggccacgga tcatcgacgc cggtgaccgg cattgtcaag gcgacggcgg tcctccacgc
     5581 gagcgcgcgc acgccggacg ttttcgatcg gatcggcctg cggaccgcgt tgcgcgcgcg
     5641 cccgggctcg ggtccgatgc tcggacacaa ccctcggcag acacgcgcac cccggcgcgg
     5701 gttgcaagcg accgccaggc gatgacagcg gacccgatgt gggatctgta cgctcactgc
     5761 acgcgtggtg gaatttccgc cagcgtcgag accgtggcgc acggtgcggc gttgtgtgag
     5821 gccgttcgct tgacttgggg tccccggttc ctatgatcgc tcccgatgaa tcgacgccat
     5881 cccgtcgagg tgctcgtggt ctatctctcg atctcggcga tcgcggcggt cgcggcgttg
     5941 ggggtcgccg ggtgctccga caacccggtg gggcggctct gcgacctcgg tgcctcgacc
     6001 ccgacggtcg actccgtggt ggtcgcgtcg ccatcgctcg attgcgtgtc gcgcacctgc
     6061 ctgcactaca agccgaaccg gccgccgccg cccggcggca acccgcagcc gatcaacagc
     6121 ggcctgtgca ccgccgagtg cagcgccgac agcgactgcg atcgcgtgcc ggagtcgccg
     6181 tgcatcaccg gcttcacgtg cgccatcgcg gtcaccgccg gcccgttctg ctgcaagaag
     6241 ttctgcatct gcaaggacta catcctgctg ccaccgtcgg ggcagctcga ggcgcccgcg
     6301 gcgtgcgatg cgacgatgtc gatcaacgcg tgctgcaacc tcgaggggcg caacaacaac
     6361 ccgagctacc cgctctgcaa gagctgaggc aagggggcgc cgcgcctcga gaggcgtcgg
     6421 atgaaggggg gaaggggcga ttcgggacgc aagcggtgtg acgttgacgg tttgacgtcg
     6481 acgttgaccc gaacgttgag gttgagggca acgtacgacc tagaccccac cgtcgacctc
     6541 gacctcggtt cgtcttctcc ggagtcaagg tgcaaggtca agggtggggt cagacgtcta
     6601 cgttgccgtc gacgtcaagg ttgtggtcaa ggtcatcgtc gaggacaagc ccggaattgc
     6661 cggaccggct tccgtcctgt ttcatcgacg cctcttcagg cgcggaacgc gtccttgagc
     6721 aggatcggct cgcccgcggt gatcgcgacc acgtcgaacc ggcagcgctc gaagcgcggc
     6781 gcggcgatcg ccaggtagtg ccgggcgacc cgcacgaccc ggcgctgctt ggtccgcccg
     6841 atcatctcga cggcgtggcc gtgatcgccg tccgatcggc tgcggacctc gacgaacacc
     6901 aggacgtcgc cgtcgcgcgc gacgatgtcg agctcgccgg ccttgcagcg gaagttgcac
     6961 tcgacgatct cgtacccggt ggcgagcagg agctcggtcg cggtgcgctc ggcttcgtcg
     7021 cctcgctgtt tcgtgttcac gacgcacccc ctcggtcctg ccggcgctgc acgaaccgcc
     7081 gccggccgcg acgcgctacg cggtcttctt ttctttcttg tccttcttgt ccttcttgcg
     7141 ctctttcttc ttgggctggg ccttggctcc gtcggccgcg ggcgcctcgc cctcggcggc
     7201 gggggcggcg gccgcagccg gcggcgcctc gacgacctcg gtgcgctgct cctcggcagt
     7261 gccctcgcgc tccttgatgc gcgccgcctt gcccgacaga tcgcgcaggt aatagagctt
     7321 cgactggtgg acgcggccgc gcgacacgac ctcgatcttg tcgatgttcg gcgagttgtc
     7381 ggggaagatt cgctcgacgc cgacgccgta cgagatcttg cggacggtga acgtcgaccg
     7441 cgcgcccctg gagacccggc ggatgcacac gccctcgaag gcctgcaggc gggtcttctc
     7501 gccctcgcgg atcttcgccc agaccttgat cgagtcaccc gggcgaaact cgggaaggtt
     7561 cgcctggcgg cgttgtgact tatcgatgga ctcgaggatc cgatgcgcgc tcatgggtac
     7621 gatccgggaa agagtagctg ggacgcaggg ttctgccaga gatccccccc ggccgtcaag
     7681 ccgcgcgacc ggttcgctca ccggccgaac aggcggtcga gcaggatcgc acctgccgag
     7741 cggaccgaca ggtggttcca gtctgacgtg ccctcgatcg gtgcaagcac gcgagaaacc
     7801 gatggaatca gcgggtcggc caggccccag ccggtgccga acaggatcag gagcggcgcg
     7861 ggatccaccc cggcctcggc gcgcagcgcc tcgggggacc gccgcaccac gccggggaac
     7921 gactcggtgc gcgccgatgt cgcaaccacg cgcggggcgg tgccatggct tgccgcgatc
     7981 tcggcgatca ccgcctcgat cgactccgcg gtgcgcacca gggccagggc gcgggcgcgg
     8041 tgctcgccgt gcgcgtcgcc gatccacagc gcggcgatgt gcgccgcctt gtcgcgctgc
     8101 gcggcgatcg gtgtgacgat gtggtagccg gcgagcccgt aggtcagcga cgagcgcgcc
     8161 aggtcgtgga cgtcgaagtt ggtgagcgag gtggtgacca cgctgccggt gcgatcggtg
     8221 accgggtggt ggaccagcgc gaggtgggtt cgggcgtgga gcgccggcgg caagagcttg
     8281 cgatcggcgg cggtcggccg gaaccgggcg aacagatcgg gccggcgctg cgcggtg
//
LOCUS       DASVOR010001315         2272 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_218178,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001315 DASVOR010000000
VERSION     DASVOR010001315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218178"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1012
                     /locus_tag="VF516_25105"
     CDS             137..1012
                     /locus_tag="VF516_25105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEX8111042.1"
                     /translation="MSVRSLLRAKRDPRPALRDSPAGPVVKWVGGKTKLLPELLARMP
                     VRFGRYYEPFAGGAAMFFRVAPERAVLADSNSDLIGLYTCLANDVAGVIRRLEHHRAA
                     HAEAHYYATRTRWNDREASWSSADRAATFIYLNKTCFNGLWRVNRAGAFNVPIGRYTD
                     PPICVPQALRAASALLAGAVLRCADYRAAISDARRGDFVYFDPPYDPVAPTANFTSYT
                     TDAFGADDQRALADVARGLVGRGCQVMLSNSDTPFIRSLYKGFRIDRVKCSRAINSNA
                     ARRGEVDEVIITAGY"
     gene            complement(1035..1340)
                     /locus_tag="VF516_25110"
     CDS             complement(1035..1340)
                     /locus_tag="VF516_25110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111043.1"
                     /translation="MTNLWVLLVQNDGELTAQDAEGRVPMLARTGNDQKYLLAFKNVA
                     KARQFLSSQDVQDAEPRMVVRANHDDIVRIARSAGVVGTLIDYDPRTQKYASAGALA"
     gene            1529..>2272
                     /locus_tag="VF516_25115"
     CDS             1529..>2272
                     /locus_tag="VF516_25115"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8111044.1"
                     /translation="MAIVRLPVIGGAAPGTFRSDAGSASALPADPLLDNFSRRIRYLR
                     VSVTDRCNYRCSYCMPEELGDQLVFEPRAAVLTFEELERLIGLFARFGVRKVRLTGGE
                     PTVRKGIVELAGRIARLAGIEQVVMTSNGHLLHELAAPLAAAGVSGINLSLDTLDAAK
                     FRALTGRGDLARVLAGLDAAVAAGMRVKTNAVALHGVNDDELVALCEHAWSRGAVPRF
                     IEHMPMSDGALYAPEAELPAAAIRRALEAA"
BASE COUNT          300 a          782 c          799 g          391 t
ORIGIN      
        1 cggaccgccc ggtgcgcggt ccggtgtgtc cgccgtccgg acaccgggcg atcgcttatc
       61 gaagtacctc tcgaagtgca cgccgacgct gccaaaaagt gcatctcggg gttcgacgga
      121 cccgacgatc accgacatgt ctgttcgttc gttgttgcgt gcgaagcggg atccgcgccc
      181 ggcgctccgg gactcgccgg cgggtccggt cgtcaagtgg gtcggcggca agaccaagct
      241 cctgcccgag ctgctcgccc ggatgccggt gcggttcggc cgttactacg agccgttcgc
      301 cggcggcgcc gcgatgttct tccgcgtcgc gcccgagcgc gccgtgctcg ccgattccaa
      361 ctccgacctg atcgggctct acacctgcct ggcgaacgac gtcgccgggg tgatccgccg
      421 gctcgagcac caccgcgcgg cgcacgccga ggcgcactac tacgcgaccc gcacgcggtg
      481 gaacgatcgc gaggcgtcgt ggagctcggc cgatcgcgcc gcgacgttca tctacctcaa
      541 caagacctgc ttcaacggcc tgtggcgggt caaccgcgcc ggcgcgttca acgtgccgat
      601 cggccgctac accgatccgc cgatctgcgt gccccaggcg ctgcgcgctg cgagcgcgct
      661 gctcgccggc gccgtgctgc gctgcgccga ctaccgcgcc gcgatcagcg acgcccggcg
      721 cggcgacttc gtctacttcg atccgccgta cgatccggtc gcgccgaccg ccaacttcac
      781 cagctacacc accgacgcgt tcggcgccga cgatcagcgc gcgctggccg acgtcgcccg
      841 cggcctggtc ggccgcggct gccaggtcat gctgtccaac agcgataccc cgttcatccg
      901 gtcgctgtac aagggcttcc ggatcgatcg cgtcaagtgc agccgcgcga tcaactccaa
      961 cgccgcccgc cgcggcgagg tcgacgaggt gatcatcacc gccggctact agccggcgat
     1021 gggtcggcgc ctgctcaggc cagggcgccg gcgctggcgt acttctgcgt cctggggtcg
     1081 tagtcgatca aggtgccgac cacgccggcg gacctggcga tccgcacgat gtcgtcgtgg
     1141 ttggcgcgca cgaccatgcg cggctcggcg tcctggacat cctggctcga caggaactgc
     1201 cgggccttgg cgacgttctt gaaagccagc aggtacttct ggtcgttgcc ggtccgggcc
     1261 agcatcggga cccggccctc ggcgtcttgt gcggtcagct caccgtcatt ctgaacgagt
     1321 aggacccaca ggttggtcaa gggcgtggtg tccgttcggc tctgtgtcat caaggctgcg
     1381 cgttctcctg ctccaggttc tgagagcggg ggatagatgc ggtttttgtc gcgatctgtc
     1441 aagggctttg cgtttgcgtt gcggtaacga gatcgcgcgc ttgacgtagg ctcgctcaca
     1501 cggtccactg cggttgcggt ttcttgccat ggcgattgtc cgtctgccgg tgatcggtgg
     1561 ggccgctccg gggacctttc ggagcgacgc cgggtccgct agcgcgctgc ccgcggaccc
     1621 gcttctggat aatttttctc gtagaatcag gtacctgagg gtctcggtca ccgatcgctg
     1681 caactaccgc tgctcgtact gcatgcccga ggagctcggc gatcagctgg tgttcgagcc
     1741 ccgggcggcg gtgctgacgt tcgaggagct ggagcggttg atcgggctgt tcgcgcggtt
     1801 cggcgtccgc aaggtccggt tgaccggcgg cgagccgacc gtgcgcaagg ggatcgtcga
     1861 gctggccggc cggatcgcgc ggctggccgg catcgagcag gtcgtgatga cgtcgaacgg
     1921 ccacctgctg cacgagctgg cggcgccgct cgcggcggcc ggggtgtcgg ggatcaacct
     1981 ctcgctcgac acgctcgacg cggcgaagtt ccgcgcgctg accggacgcg gcgacctcgc
     2041 gcgcgtgctg gccgggctcg acgccgcggt cgcggcgggg atgcgggtca agaccaacgc
     2101 ggtggcgctg cacggggtca acgacgacga gctggtcgcg ctgtgcgagc acgcgtggtc
     2161 gcgcggcgcg gtgccgcggt tcatcgagca catgccgatg tccgacggcg cgctgtacgc
     2221 gcccgaggcc gagctcccgg ccgcggcgat ccggcgcgcg ctcgaggccg cg
//
LOCUS       DASVOR010001316         3693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43051,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001316 DASVOR010000000
VERSION     DASVOR010001316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43051"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..915
                     /locus_tag="VF516_25120"
     CDS             <1..915
                     /locus_tag="VF516_25120"
                     /inference="COORDINATES: protein motif:HMM:TIGR04085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8111045.1"
                     /translation="FHVALGGGESAVLPWLGELADHARRRGIVPNLTTSGLAGLDRLV
                     AIAGRFGQINVSLDGLGATYARVRGFDGFARADAAIRALRRVKREVGINVVVTRGNFD
                     ELDGLFAYAARHRLSEVELLRFKPSGRGARAYDELRCSDAQHRALLPAVLAAARRHAV
                     RVKVDCSYTPMLAHHRPDRALLAELCVYGCTGGDFLVGAKPGGQLTACSFAAPPPAVA
                     DAARPRITELRGYWETRDAFGAFRRWRDAREPCASCEYHALCRGGCKVVSAHVTGDPA
                     APDPECPRVIDHAGRDRRDDRVRLHVVP"
     gene            1034..1864
                     /locus_tag="VF516_25125"
     CDS             1034..1864
                     /locus_tag="VF516_25125"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HEX8111046.1"
                     /translation="MKTVMIISDATGETAERMVRAATLQFSEPVQIRLHSRVRLESEL
                     EQILEKAAELRALVVFTVVNAEERDLIGKLVERYNLETVDLIGALISKLAMFLGSAPA
                     GVPGLLHSITDDYFRRIEAVEFAVKNDDGAEPRNLPKADLVLVGISRTSKTPLSTYLA
                     QRGLKVANVPLVLGVDPPEELSQVDDRKVFGLIVQPDMLMRIRQARLTHLGMPQDSSY
                     GQRAHIDNEIAYSREIFRKHANWPVIDVTNRAIEETAADILRIYQERIEHSSHASGTG
                     "
     gene            complement(1905..2585)
                     /locus_tag="VF516_25130"
     CDS             complement(1905..2585)
                     /locus_tag="VF516_25130"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8111047.1"
                     /translation="MTADYFVNHARARRFPWTLYHQPLERDLARFLRQVAAEHSRGEV
                     LIVGCGLLHEIDAAPGGLSFHVADIDERAVAAVLARCDPRITGGTVVDPEQPIDQSFG
                     QGFGQGFGQGFGSARRFAAIYAKEVVEHVRAWPSWLTGLRRALVPSGRLWLSTPNYGE
                     PWLPALESTVLELIARRSGFSRRDLHPTRFSRRSLARGLGSAGFEQVEVRVAPNRLAL
                     TAWARAGH"
     gene            complement(2582..3442)
                     /locus_tag="VF516_25135"
     CDS             complement(2582..3442)
                     /locus_tag="VF516_25135"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8111048.1"
                     /translation="MTRLSEDLHPPGDEDIERWILAYLEHAPLSLTLREINRLIAIEA
                     VASERRPVLDVGCGDGFWWTLRGGDRREIYGIDISASEIAQARTRIHAVLTDVSRERP
                     FPGTEFAEIIGNCSLEHVPDIDAALSNLRQAAAPGGRLLMFVPTPRWAYQGRMQSWLL
                     ARAPRIAMSLSGALNGFFQHWHLYDAKVWTRLLAQNGWQVRAAYGLGSARSEFLFRAF
                     LPPAFLEFVAKRVTGFYPSKLARFLPRAALAPAVRLVRWAVSDPLVPVDSPTAYEYLL
                     IADAAELPAR"
BASE COUNT          532 a         1341 c         1365 g          455 t
ORIGIN      
        1 tttcacgtcg cgctcggcgg cggcgagagc gcggtgctgc cgtggctcgg cgagctcgcg
       61 gaccacgcgc ggcggcgcgg catcgtcccc aacctgacga cgtcggggct cgccgggctc
      121 gaccggctgg tcgcgatcgc cgggcggttc ggccagatca acgtctcgct cgacgggctg
      181 ggcgcgacct acgcgcgggt gcgcgggttc gacgggttcg cgcgcgccga cgcggcgatc
      241 cgcgcgctgc gccgggtcaa gcgcgaggtc ggcatcaacg tggtggtgac ccgcggcaac
      301 ttcgacgagc tcgacgggct gttcgcgtac gcggcgcggc accggctgtc ggaggtcgag
      361 ctgctgcggt tcaagccgtc gggccgcggc gcgcgggcct acgacgagct gcgctgtagc
      421 gacgcgcagc accgcgcgct gttgcccgcc gtgctcgccg cggcgcggcg ccacgcggtg
      481 cgggtcaagg tcgactgctc gtacacgccg atgctcgccc accaccgccc cgatcgcgcg
      541 ctgctcgccg agctctgcgt gtacggctgc accggcggcg acttcctggt cggcgccaag
      601 cccggcggtc agctcaccgc gtgcagcttc gccgcgccgc cgccggccgt ggccgacgcc
      661 gcacgcccgc ggatcaccga gctgcgcggc tactgggaaa cgcgcgacgc gttcggcgca
      721 ttccgccgct ggcgcgacgc gcgcgagccg tgcgcgagct gcgagtacca cgcgctgtgc
      781 cgcggcggct gcaaggtcgt cagcgcgcac gtcaccggcg atccggccgc gccggatccg
      841 gaatgcccgc gcgtgatcga tcacgccggg cgcgaccgcc gggacgatcg cgtgcgcctg
      901 cacgtcgtac cgtgagcgat ggcggacgac tggcttggct cgccgccgag cgcgcgcggc
      961 tcgacgacga gcgtcgccag ggcccgatcg ggacaggaga cggtggtcgc acgggcatgc
     1021 tatagagcag ccgatgaaga cggtcatgat catcagcgac gccaccggcg agaccgccga
     1081 gcgcatggtc cgcgcggcga cgctgcagtt cagcgagccg gtgcagatcc gcctgcactc
     1141 gcgcgtccgg ctggagagcg agctggagca gatcctggag aaggcggccg agctccgcgc
     1201 gctcgtggtg ttcaccgtgg tcaacgccga ggagcgcgac ctgatcggca agctggtcga
     1261 gcgctacaac ctggagaccg tcgacctgat cggcgcgctg atcagcaagc tcgcgatgtt
     1321 cctcggctcg gcccccgccg gcgtgccggg cctcttgcac tcgatcaccg acgactactt
     1381 ccgccggatc gaggcggtcg agttcgcggt caagaacgac gacggcgccg agccgcgcaa
     1441 cctgcccaag gccgacctcg tgctggtcgg gatctcgcgt acgtcgaaga cgccgctgtc
     1501 gacctacctc gcgcagcgcg gcctcaaggt cgccaacgtg ccgctggtgc tcggcgtgga
     1561 cccgcccgag gagctgtcgc aggtcgacga ccgcaaggtg ttcggcctga tcgtccagcc
     1621 cgacatgctg atgcggatcc gccaggcccg gctgacgcac ctcgggatgc cgcaggactc
     1681 gagctacggc cagcgcgccc acatcgacaa cgagatcgct tactcgcgcg agatcttccg
     1741 caagcacgcc aactggccgg tcatcgacgt caccaaccgg gcgatcgagg agaccgccgc
     1801 cgacatcctg cggatctacc aggagcggat cgagcactcg tcgcacgcgt ccggcacggg
     1861 ctgagccggc cgccgccgac gctcggcgac ggctcagcga tggctcagtg cccggcgcgc
     1921 gcccacgcag tcagcgcgag ccggttcggc gcgacgcgga cctcgacctg ctcgaacccc
     1981 gcgctgccca ggccgcgcgc cagcgatcgc cgcgagaacc gggtcgggtg gagatcgcgc
     2041 cgggagaacc ccgagcgccg ggcgatcagc tccagcacgg tggactccag cgccggcagc
     2101 cagggctcgc cgtagttcgg ggtcgacagc cacagccggc cgctcggcac cagcgcgcgg
     2161 cgcagcccgg tgagccagct cggccacgcc cgcacgtgct cgacgacctc cttggcgtag
     2221 atcgcggcga accggcgggc tgaaccgaag ccctgcccga agccctgccc gaagccctgc
     2281 ccgaagctct gatcgatcgg ctgctcgggg tccaccaccg tgccgccggt gatccgcgga
     2341 tcgcaccggg cgagcacggc ggcgaccgcg cgctcgtcga tgtcggcgac gtggaacgaa
     2401 aggccgcccg gcgcggcgtc gatctcgtgg agcaggccgc agccgacgat cagcacctcg
     2461 ccgcgcgagt gctcggccgc gacctggcgg aggaaccgcg cgaggtcgcg ctcgagcggc
     2521 tggtggtaca gcgtccacgg gaaccgccgc gcccgcgcgt ggttgacgaa gtagtcggcc
     2581 gtcatcgcgc cggcagctcc gcggcgtcgg cgatcagcag gtactcgtac gcggtcgggg
     2641 aatcgaccgg gaccagcgga tcggacaccg cccagcgcac cagccggacc gcgggggcga
     2701 gcgcggcgcg cggcaggaag cgcgcgagct tcgacgggta gaacccggtg acccgcttgg
     2761 cgacgaactc gaggaacgcg ggcggcagga acgcgcggaa caggaactcc gagcgcgccg
     2821 agcccaggcc gtacgcggcg cggacctgcc agccgttctg ggccaggagg cgggtccaga
     2881 ccttggcgtc gtacaggtgc cagtgctgga agaagccgtt gagcgcgccg gacaggctca
     2941 tcgcgatccg cggcgcgcgc gcgagcagcc acgactgcat ccggccctgg tacgcccagc
     3001 gcggggtcgg cacgaacatc aggagccggc cgccgggcgc cgccgcctgc cggaggttgg
     3061 acagcgccgc gtcgatgtcg ggcacgtgct ccagcgagca gttgccgatg atctcggcga
     3121 actcggtccc cgggaacggc cgctcgcggg acacgtcggt cagcaccgcg tggatccggg
     3181 tccgggcctg ggcgatctcg ctcgccgaga tgtcgatgcc gtagatctcg cggcgatcgc
     3241 cgccgcgcag cgtccaccag aagccgtcgc cgcagccgac gtcgagcacc ggccgccgct
     3301 cgctggcgac cgcctcgatg gcgatcagcc ggttgatctc gcgcagggtg agcgacagcg
     3361 gcgcgtgctc caggtaggcg aggatccagc gctcgatgtc ctcgtcgccc ggcgggtgca
     3421 ggtcctcgga gagtcgggtc acggggatcg gatcggaggc agcgcgagcc acgcccctgc
     3481 ttaccattgc cggccggcgg cgtcgcccgg atccccggcg ccgcctcacg gccccaggcg
     3541 ggcgcggaac ggcagctcgc gcgccggatc gggcgtgcga tcgaagtaca gccggcggaa
     3601 ccgctcccgg tcgcggcgga cgtcggcgtc ggaccggctc gccatcccgg cgatgtaggc
     3661 gtcgatctgc gccgggacgt cggggacgcg gac
//
LOCUS       DASVOR010001317         1919 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_247556,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001317 DASVOR010000000
VERSION     DASVOR010001317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247556"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..855
                     /locus_tag="VF516_25140"
     CDS             <1..855
                     /locus_tag="VF516_25140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HEX8111049.1"
                     /translation="CHVVPSRWDDEGHILAGGVTITGPAKVTFGARAALTIAPADRKG
                     LPTFQDGKCSNVYCHGDALHAAGGSATEPRWDDPAPAGACDRCHGNPPPSHAQTQCAT
                     CHPSAPHIDGVVQVGRGPGCSGCHGDATSPAPPTDLSVPANTAITAIGVGAHRAHLTV
                     PSGLRGPIPCETCHLVPTQVNDPGHIDSPPPAEVNASLSWDRTTQTCITNCHVASRPV
                     WTTRGSVVCGSCHGIPPSGSPHTPTMTLASCATCHPQTVDAFGTILLTTGPGGTTSKH
                     MNGVVDVQ"
     gene            845..1528
                     /locus_tag="VF516_25145"
     CDS             845..1528
                     /locus_tag="VF516_25145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111050.1"
                     /translation="MFNDPYTFPYTAFALGCAGLATALLLWYLIARPQLGRATKIVLL
                     LGIGVLPLGTAANGNVAGFTATKSRTFCGACHVMTPFNDDAGSAGSTSLAARHSRNEE
                     FGGEACYMCHADYGMFETVVTKIGGMRHVYEYALHFRQLSVDEALPRIHIRRPFPNAT
                     CMRCHSTENPGWRRVGDHKSLLDRVRAGEVSCASNGCHGPAHPFAQVAHLKAEQAEAA
                     RAIENGGAR"
     gene            1525..>1919
                     /locus_tag="VF516_25150"
     CDS             1525..>1919
                     /locus_tag="VF516_25150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111051.1"
                     /translation="MSKPRKPLDERREEAGGGDPAGLAGGAGGGAPRGRDPIGVIGRR
                     TRGILVVAAIMTLIGLGLMVWSMAQPTPMPVILAMSVGQGLGILAFLLFGVVVLVDQL
                     RKQREAGAHDVAAQSDGIPRAAPDEFPVPA"
BASE COUNT          276 a          742 c          661 g          240 t
ORIGIN      
        1 tgccacgtcg tcccgtcgcg ctgggacgac gagggccaca tcctggcggg cggcgtcacg
       61 atcaccggcc cggccaaggt gacgttcggc gcgcgcgcgg cgctcacgat cgccccggcg
      121 gatcgcaagg gcctacccac cttccaggac ggcaagtgca gcaacgtgta ctgccacggc
      181 gacgccctgc acgccgccgg cggctccgcg accgagcccc ggtgggatga cccggcgccg
      241 gcgggagcct gcgatcgctg ccacggcaac ccgccgccca gccacgcgca gacccagtgc
      301 gcgacctgcc atcccagcgc gccgcacatc gacggcgtcg tccaggtcgg ccgcggcccg
      361 ggctgctcgg gctgccacgg cgacgcgacc tcgccggccc cgccgaccga tctgtcggtg
      421 ccggccaaca cggcgatcac ggcgatcggc gtcggtgccc accgcgccca cctcacggtc
      481 ccctcgggcc tgcgcggccc gatcccgtgc gagacctgcc acctcgtgcc gacccaggtc
      541 aacgacccgg gccacatcga ctcgccgccg ccggccgagg tcaacgccag cctgtcgtgg
      601 gaccgcacca cgcagacctg catcacgaac tgccacgtcg cgtcgcggcc ggtgtggacc
      661 acccggggca gcgtggtctg cggcagctgc cacggcatcc cgccctcggg cagccctcac
      721 acgcccacga tgacgctcgc gagctgcgcg acctgtcacc cccagaccgt cgacgcgttc
      781 gggacgatcc tcctcacgac tggtccgggt gggacgacca gcaagcacat gaacggagtg
      841 gtcgatgttc aatgacccct acaccttccc gtacaccgcg ttcgcgctcg gctgcgccgg
      901 gctcgccacc gcgctgctgc tctggtacct gatcgcccgg ccccagctcg gccgcgccac
      961 gaagatcgtg ctgctgctcg gcatcggcgt cctgccgctg ggcacggcgg ccaacggcaa
     1021 cgtcgccggg ttcaccgcga ccaagagccg gacgttctgc ggcgcgtgcc acgtgatgac
     1081 gccgttcaac gacgacgccg gcagcgcggg ctcgacctcg ctcgccgcgc gccactcgcg
     1141 caacgaggag ttcggcggcg aggcctgcta catgtgccac gccgactacg gcatgttcga
     1201 gaccgtcgtc accaagatcg gcggcatgcg gcacgtctac gagtacgcgc tgcacttccg
     1261 ccagctcagc gtcgacgagg cgctgcccag gatccacatc cgccggccgt tccccaacgc
     1321 gacctgcatg cggtgccact cgacggagaa cccgggctgg cgccgggtcg gcgatcacaa
     1381 gagcctgctc gatcgggtgc gcgccggcga ggtcagctgc gcgagcaacg gctgccatgg
     1441 cccggcgcac ccgttcgccc aggtcgcgca cctcaaggcc gagcaggcgg aagcggcccg
     1501 ggcgatcgag aacggaggcg cgagatgagc aagccacgca agccgctcga cgagcgaagg
     1561 gaggaggcgg ggggtgggga ccctgccggc ttggccggcg gggcgggggg cggagccccc
     1621 cgtgggagag acccgatcgg cgtgatcggc cggcggaccc ggggcatcct ggtcgtcgcg
     1681 gcgatcatga cgctgatcgg cctcggcctc atggtctggt cgatggccca gccgacgccg
     1741 atgccggtga tcctggcgat gtcggtcggc caggggctcg gcatcctcgc gttcctcctg
     1801 ttcggggtcg tcgtcctggt cgaccagctg cgcaagcagc gcgaggccgg cgcccacgac
     1861 gtcgccgcgc agtccgacgg catcccccgg gccgcgcccg acgagttccc ggtgcccgc
//
LOCUS       DASVOR010001318         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_14362,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001318 DASVOR010000000
VERSION     DASVOR010001318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14362"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            571..2106
                     /locus_tag="VF516_25155"
     CDS             571..2106
                     /locus_tag="VF516_25155"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8111052.1"
                     /translation="MTPIETSGEPPSGGAQRASDPSIGIATGADAAAGGGTVPGSIPP
                     PISGAVARSRSQTWFGEGGPLRRFVTRWGFPLFVLLILFLGREVLLPFVFAGLIAYIL
                     TPVVHWMVDRADGTRRMPRGVAIVVCYLVFISLVAGFLFLLVPRLSRDVARLGKEAPA
                     LYKRINEEWTPEVARWLEGRFPSLSRPKPVPVVEEPAAPSDVALPPGTAFTMTPLPEG
                     GGYAMQLAPNGIDVKPQADGSYHIQTVETRPEPASLEDKLRSFVKKTLLGLQDKLNDV
                     VRLGQSLIVGFIRGIFLFFFTLMIGAFVLVDLEKVHGFLRSLFPANVRDDYDVIIAGI
                     DRGLSGVIRGQLVICVINSLFTYAGLLTFGVKYKLILAVVAGVMSLIPIFGSILSSVP
                     IVIVALVSGDEGIDIFRGVAMTLWIIGIHFIEANVLNPKIIGTAAKIHPVLVIFSLFL
                     GEHAFGLVGALLAVPVLSAISVVFMYLYRKAWKDAPRAPGRGGSGPHAKPADPASIAA
                     PPT"
BASE COUNT          303 a          739 c          815 g          363 t
ORIGIN      
        1 cgccacgctc gacgccgggt tgaacgaggc cgagctggcg agcgccggca cgcggcgatc
       61 gctgctcggg cgggtcggcg gctggttctc ggcccgctgg cggcgcgacg caccgtgacg
      121 gggcgacggg gacgacccaa ccgagccggg gtgaagaggt gtccttggaa ggccgggaac
      181 gtgcttgcga agacgttgac gtcgaccttg acgctgtcgc aaggcgtcga cctgaacggc
      241 gacggcgacg tagactccat cctcgacgtt ggaccttgtc cggagacctc ctggtggtct
      301 ccgaatcctc gtcgaagcat cgcggccgat ggtcaaagcg gaggtcgacg atgggctcga
      361 cgatgggctc gacgtccacg gccacgtcaa gctcaacgac ggccgtcaac gtcgagggtc
      421 aggtcgacgt ccaccggacg ctggcccccg gaggtaaggc cccaacctct ggtcgacgcc
      481 tctgagccgg ggtgagccgg ggtgagccgg gcggagggag acgcacgcgc gggccggtcc
      541 gcggtggggc aggtctgcta tacccgcgcg gtgaccccga tcgagacctc cggtgagcca
      601 ccgtccggcg gcgcgcagcg cgccagcgac ccctcgatcg gaatcgccac cggcgcggac
      661 gcggcagcgg ggggcgggac ggtccccggc tcgatcccgc cgccgatctc gggcgcggtc
      721 gcgcgcagcc ggtcgcagac ctggttcggc gagggcgggc cgctgcggcg gttcgtgacc
      781 cggtggggct tcccgctgtt cgtgctgctg atcctgttcc tcgggcgcga ggtgctgctg
      841 ccgttcgtgt tcgccgggct gatcgcgtac atcctcacgc cggtggtgca ctggatggtg
      901 gatcgcgccg acggcacgcg ccggatgccg cgcggcgtgg cgatcgtggt gtgttacctg
      961 gtgttcatct cgctggtcgc cggcttcctg ttcctgctcg tgccccggct gtcgcgcgac
     1021 gtggcgcggc tgggcaagga ggcgccggcg ctgtacaagc ggatcaacga ggagtggacg
     1081 ccggaggtcg cgcgctggct cgagggccgg ttcccgtcgc tgagccggcc caagccggtg
     1141 ccggtggtcg aggagccagc ggcgccgtcc gacgtcgcgc tgccgcccgg caccgcgttc
     1201 acgatgacgc cgctgcccga aggcggcggc tacgcgatgc agctcgcccc caacgggatc
     1261 gacgtcaagc cgcaggccga cgggtcgtac cacatccaga ccgtcgagac ccggcccgag
     1321 ccggcgtcgc tcgaggacaa gctgcgctcg ttcgtcaaga agacgctgct cggtctgcag
     1381 gacaagctca acgacgtggt ccggctcggc cagtcgctga tcgtcggctt catccgcggc
     1441 atcttcctgt tcttcttcac gctgatgatc ggcgcgttcg tgctggtcga cctcgagaag
     1501 gtgcacggct tcctgcgcag cctgttcccc gccaacgtgc gcgacgacta cgacgtgatc
     1561 atcgccggca tcgaccgcgg gctatcgggc gtgatccgcg gccagctggt gatctgcgtg
     1621 atcaacagcc tgttcaccta cgccgggctg ctgacgttcg gcgtgaagta caagctgatc
     1681 ctcgcggtgg tcgccggggt gatgagcctg atcccgatct tcggatcgat cctgtcgtcg
     1741 gtgccgatcg tgatcgtcgc gctggtgtcg ggcgacgagg gcatcgacat cttccgcggc
     1801 gtcgcgatga cgctgtggat catcgggatc cacttcatcg aggccaacgt gctcaacccc
     1861 aagatcatcg gcaccgcggc gaagatccac ccggtgctgg tgatcttctc gctgttcctc
     1921 ggcgagcacg cgttcggtct ggtcggcgcg ctgctcgcgg tgccggtgct gtcggcgatc
     1981 tcggtggtgt tcatgtacct gtatcgcaag gcctggaagg acgcgccgcg cgcgccgggg
     2041 cgcggcggca gcggcccgca cgccaagccg gcggatccgg cgtcgatcgc ggccccaccg
     2101 acctgacggc cgcggcgcgg ggcggttcat cgtcgccgcg tgatctgacg catgggatct
     2161 ggcagcgaca cacgggggcg gacgccgcgg tgccgacgat gatgcaggtt gaagcagcgg
//
LOCUS       DASVOR010001319         4513 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_71906,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001319 DASVOR010000000
VERSION     DASVOR010001319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4513
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71906"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            340..1158
                     /locus_tag="VF516_25160"
     CDS             340..1158
                     /locus_tag="VF516_25160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111053.1"
                     /translation="MRAQRIHTVVAAGVDNPALIVRWRAEPELLRGHGVEPSQVDLDA
                     LWRFAGLTVKVRHNALREELPASFRLMNLAGLEIELFASYASERAAQGARLAPTTEGR
                     TLDLLGFLEGWLDRDNTAHALLWDLIRHERALGWLARTAPAVGSSPDLRRGEPPTPPA
                     ADVVPRIRGKIVLHEMTSDPQAVARALEDRMPDLAAIPRTQSRVCYWRTAGPEVSILQ
                     LDELAFGTLGLVDGKRTAAEVYRALGGGRRPPPSFLRILGELQTIGIIALRRPR"
     gene            1183..1866
                     /locus_tag="VF516_25165"
     CDS             1183..1866
                     /locus_tag="VF516_25165"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEX8111054.1"
                     /translation="MAKSPEDGLAPGPDGKPRCFWAVSAPDYVDYHDREWGFPVVDDR
                     RLFEKLCLEGFQAGLSWLTILRKREAFRRAFAGFDPEAVARFGARDVTRLLADPGIVR
                     HRGKIESAIHNAARALELIDERGSLAAYVWSFVPAAGERPARVTREALRAMTQTPTSV
                     ALSKDLKCRGWTFVGPTTIYAFMQAMGLVDDHLEGCVIRRAAEAARKKPPGLPAVAAP
                     RPRTRGAAK"
     gene            1924..2475
                     /locus_tag="VF516_25170"
     CDS             1924..2475
                     /locus_tag="VF516_25170"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX8111055.1"
                     /translation="MRTLLVLTAVAALPGVTRADPPGTHDAPAAGHDPVLQVTSSAFG
                     ANQEIPPKYTCDGTQTTPPLAWSRVPAGTQSVAILIEDPDAPSGTFTHWLVTGIPPTT
                     TALAGGAALPQGATAARNGKGRLGYDGPCPPDGRHRYVFRVFALDTAIPPPKDRDGFL
                     SAIHGHVLAEGRLIGTYQRTAPP"
     gene            2645..3814
                     /locus_tag="VF516_25175"
     CDS             2645..3814
                     /locus_tag="VF516_25175"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8111056.1"
                     /translation="MPTNGHGRLRIAQVAPLYESVPPRLYGGTERIVSYLTEELVAEG
                     HDVALFASGDSQTSARLVAGCARALRLASCADPIAPHLAMVEHVARRARDFDVVHFHI
                     DYLHFPVSSRERYRCVTTLHGRLDLPDIVPVFARFADLPVVSISDAQRAPLPDLAWQA
                     TVHHGFPEDLYRFEPRPGDYLAFLGRVSPEKGLDRAIEIARRTGHRLRIAAKIDRADR
                     DYYTRHIAAQLRHPHVEFLGEIGERDKQELLGGALALVFPINWPEPFGMVMVEAMACG
                     TPVIAYRGGSVPEVLDHGVTGFVCDGIDQAIAAIGRVHELSRAACRRAFEQRFTAARM
                     ASDYTAIYRRILQRREPRDLRATGTHGERDDHGHDGTQHDDPRGLLHPRDQLASG"
     gene            3741..>4513
                     /locus_tag="VF516_25180"
     CDS             3741..>4513
                     /locus_tag="VF516_25180"
                     /inference="COORDINATES: protein motif:HMM:NF026092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HEX8111057.1"
                     /translation="MATTEHSTTIHEAYSILATSSRRDERTRVIKHGDSFGVFDHAGM
                     IGQAGMGELGLYHDDTRYLSMFEIALERRRPLLLHSTVLRDNVLHVDLANPDLPDRPE
                     PLVRDSVHLFAQSFLWDRGWHARLELHNYALRAVEVELSLLFDADFADVFEVRGVQRL
                     RRGAVRPPVIEPGAVALSYDGLDGVVRTTHLAFDPAPVTLTAGRASYRLPLAPQGHAS
                     IELRVAFSRSAPRPDAPPEASHDEPARPSYDRAYRCAVSA"
BASE COUNT          598 a         1668 c         1604 g          643 t
ORIGIN      
        1 atcgtccagc gcgtcgccga cgatcacggc ggccggatcc accacgagca gcggccgggc
       61 gcgacggtgt tccggctgga gctgccgatc gccggcggcg tcgccggctg accgatcgcg
      121 acgcgccggg agcgcatgcg atcacgcgcc ggtgcgcgcg agatcttcgc gtgtttcgct
      181 tccggaccgg acggtccttg acagctcacg acggcgcacg ctacggtcag aaacagcgat
      241 catggggcat cgcgaccata catgtcgcga tcctgaccat ccctggagtg gaaggtagga
      301 catgctcagg aagaccaatc gatcggtttc tcgacgggga tgagagcgca acggatccat
      361 accgtggtcg ccgccggggt cgacaacccg gcgctgatcg tacgctggcg cgccgagccg
      421 gagctgctgc gcggccacgg cgtcgagccg tcgcaggtcg atctcgatgc gctgtggcgg
      481 ttcgccgggc tgaccgtcaa ggtccgccac aatgcgctgc gtgaggagct gccggcatcg
      541 ttccgcttga tgaacctcgc cggcctggag atcgagctgt tcgcgagcta cgccagcgag
      601 cgcgccgcgc agggcgcccg gctcgcgccg acgacggagg gccggacgct cgacctgctg
      661 gggttcctcg agggctggct cgaccgcgac aacaccgcgc atgcgctgct gtgggacctg
      721 atccgccacg agcgcgcgct cggctggctc gcccgcaccg cgccggcggt gggatcgtcg
      781 ccggatctcc gccgcggcga gccgccgacg ccgcccgcgg ccgacgtcgt accgcggatc
      841 cgcggcaaga tcgtgctgca cgagatgacc agcgatccgc aggccgtcgc ccgcgcgctc
      901 gaggatcgca tgcccgatct ggcggcgatc ccgcgcaccc agtcgcgcgt ctgctactgg
      961 cgcacggcgg ggcccgaggt ctcgatcctg cagctcgacg agctcgcgtt cggcacgctg
     1021 gggctggtcg acggcaagcg gaccgccgcg gaggtctacc gcgcgctcgg cggcgggcgg
     1081 cggccgcctc ccagcttcct gcgcatcctc ggcgagctcc agaccatcgg gatcatcgcg
     1141 ctgcgccggc cgcggtgagg tcgcccggcg tatcctggcg tcgtggcgaa atctcccgag
     1201 gatggtctcg cgccggggcc cgacggcaag ccgcgctgct tctgggcggt gtcggcgccc
     1261 gactacgtcg actaccacga ccgcgagtgg ggattcccgg tggtcgacga tcgccggctg
     1321 ttcgagaagc tgtgcctcga gggcttccag gccgggctgt cgtggctcac gatcctgcgc
     1381 aagcgcgagg cgttccgccg ggcgttcgcc ggcttcgatc cggaggcggt cgcccggttc
     1441 ggcgcgcgcg acgtcacccg gctgctcgcc gatcccggca tcgtccggca ccgcggcaag
     1501 atcgagtcgg cgatccacaa cgcggcgcgc gcgctcgagc tgatcgacga gcgcggctcg
     1561 ctcgccgcct acgtgtggag cttcgtgccg gcggccggcg agcgcccggc gcgggtgacc
     1621 cgagaggcgc tgcgcgcgat gacccagacc ccgacgtcgg tcgcgctgtc caaggatctc
     1681 aagtgccgcg gctggacctt cgtcggcccg acgacgatct acgcgttcat gcaggcgatg
     1741 gggctggtcg atgaccacct cgagggctgc gtgatccgcc gcgccgccga ggccgcgcgc
     1801 aagaagcccc ccggtctccc cgccgtggct gcgccgcggc cccgcacccg cggcgcggcg
     1861 aagtgaccgc gcgctcgcgg attcaccgat cgccgaggaa tgctcgttgc acggcgatcg
     1921 agcatgcgga ccctacttgt tctgaccgcc gtggcagcgc tgcccggggt gacgcgtgcc
     1981 gatccgccgg gcacccacga cgcgccggcc gcgggccatg acccggtgct gcaggtcacc
     2041 tcgagcgcgt tcggtgccaa ccaggagatc ccgccgaagt acacctgcga cgggacgcag
     2101 accacgccgc cgctcgcgtg gtcgcgcgtg cccgccggca cgcagagcgt cgcgatcctg
     2161 atcgaggatc ccgacgcgcc cagcgggacg ttcacccact ggctggtcac cgggattcct
     2221 ccgaccacca ccgcgctggc cgggggcgcc gcgctgccgc agggcgcgac cgccgcgcgc
     2281 aacggcaagg ggcgcctcgg ctacgacggg ccgtgcccgc cggacggccg ccatcgttat
     2341 gtgttccgcg tgttcgcgct cgacaccgcg atcccgccgc ccaaggaccg cgacggcttc
     2401 ctctcggcga tccacggaca cgtcctggcc gagggccggc tgatcggcac gtaccagcgg
     2461 acggcgcctc cgtgacctcc cgatgtgcga atgcaccgcg cctcgcggcc ggcgtgtgtc
     2521 ggtggcgctc gaccgatcgg gggcagacgg cgggcgacgg cggatctcgc tcgtcgatcg
     2581 acgcctgggc gcccggcatc tcgggaactc cttctggtac gtggtttgta gccacggtga
     2641 ttcgatgcca accaacggtc acggccggct ccggatcgca caggtcgcgc cgttgtacga
     2701 gagcgtgccg ccgcggctct acggcggcac cgagcggatc gtgtcgtacc tgaccgagga
     2761 gctggtcgct gaaggccacg acgtcgcgct gttcgcgagc ggcgactcgc agacctcggc
     2821 gcggctggtc gccggttgcg cgcgcgcgct gcggctggcc agctgcgccg acccgatcgc
     2881 gccgcacctg gcgatggtcg agcacgtggc gcgccgtgcg cgcgacttcg acgtcgtgca
     2941 cttccacatc gactacctgc acttcccggt gtcgagccgc gagcgctacc gctgcgtcac
     3001 cacgctgcac ggccggctcg atctgccgga catcgtgccg gtgttcgcgc ggttcgcgga
     3061 cctgcccgtg gtctcgatct ccgacgccca gcgcgccccg ctgccggatc tcgcgtggca
     3121 ggcgaccgtg caccacgggt tcccggagga cctgtaccgg ttcgagccga ggccgggcga
     3181 ctacctcgcg ttcctcggcc gggtgtcgcc ggagaagggg ctcgaccggg cgatcgagat
     3241 cgcgcggcgc accggccacc ggctgcggat cgcggccaag atcgatcgcg ccgatcgcga
     3301 ctactacacc cggcacatcg cggcgcagct ccgccacccg cacgtcgagt tcctgggcga
     3361 gatcggcgag cgcgacaagc aggagctgct cggcggcgcg ctcgcgctgg tgttcccgat
     3421 caactggccc gagccgttcg gcatggtgat ggtcgaggcg atggcgtgcg gcacgccggt
     3481 gatcgcgtat cgcggcggct cggtgcccga ggtgctcgac cacggcgtca ccgggttcgt
     3541 gtgcgatggc atcgaccagg cgatcgcggc gatcggccgg gtccacgagc tgtcccgcgc
     3601 ggcgtgccgc cgggccttcg agcagcggtt cacggcggcg cggatggcga gcgactacac
     3661 ggcgatctat cgccgcatcc tgcaacgccg ggaaccccgg gacctccggg ccaccgggac
     3721 ccacggggag cgcgatgacc atggccacga cggaacacag cacgacgatc cacgaggcct
     3781 actccatcct cgcgaccagc tcgcgtcggg atgagcgcac tcgcgtcatc aagcacggcg
     3841 acagcttcgg cgtgttcgac cacgccggga tgatcggcca ggccgggatg ggcgagctcg
     3901 ggctctacca cgacgacacc cgctacctga gcatgttcga gatcgcgctc gagcgccgcc
     3961 gcccgctgct gctccactcg acggtgctgc gcgacaacgt gctgcacgtc gacctcgcca
     4021 accccgatct gccggaccgg cccgagccgc tggttcgcga ctcggtccac ctgttcgcgc
     4081 agagcttcct gtgggaccgc ggctggcacg cgcggctcga gctccacaac tacgcgctgc
     4141 gcgcggtcga ggtcgagctg tcgctgctgt tcgacgccga cttcgccgac gtgttcgagg
     4201 tccgcggcgt ccagcgcctg cggcgcggcg cggttcgccc gccggtgatc gagccaggcg
     4261 cggtcgcgct gagctacgac ggcctcgacg gcgtcgtccg cacgacccac ctggcgttcg
     4321 atccggcccc cgtgacgctc accgccggtc gcgcgtcgta ccggctgccg ctggcgcccc
     4381 agggccacgc ctcgatcgag ctgcgggtcg ccttctcgcg cagcgcgccg cggccggacg
     4441 ccccgccgga ggcctcccac gacgagccgg cccggccgtc ctacgatcgc gcgtaccgct
     4501 gcgcggtgtc ggc
//
LOCUS       DASVOR010001320         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_247589,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001320 DASVOR010000000
VERSION     DASVOR010001320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247589"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VF516_25185"
     CDS             complement(<1..349)
                     /locus_tag="VF516_25185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111058.1"
                     /translation="MTLSALRAALVAAVVICSDGGCAGHPMDTAEPMFGPELGQSTAP
                     GPADPTLRPPPAPGVTEASAHLEGLPAASASPTRTAQATPVEPAQPIQPTAGLIVTIP
                     APAAASAPAAAVVP"
     gene            511..1404
                     /locus_tag="VF516_25190"
     CDS             511..1404
                     /locus_tag="VF516_25190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEX8111059.1"
                     /translation="MPARSIDTATLTFGLVAIPVRIYATSERSHEIHFHLVHEGCGER
                     LHQQYVCPRHGPVDRDEIIKGYELSRGSFVELSRPELEALEAVSSDEIAIQEFVPAAA
                     VDPLLIEHTYYLGPGKGGERAYRLLRDALAAADLVGIASYAARGKQYIVELLPYETGL
                     AMLQLRYADELKPWSEVPAPPQVKPSAAEIALASKIIDNLRRATFDPSRYHDEVKDRV
                     RDLIASKAKGGEIVAPPSAERPPVTDLMAALKASLANGESRPEGARTPARRSAQGRRS
                     NGAGRASSAHRSRAPRVGAHR"
     gene            complement(1903..>2083)
                     /locus_tag="VF516_25195"
     CDS             complement(1903..>2083)
                     /locus_tag="VF516_25195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111060.1"
                     /translation="GAPTPPPGAPQAPGAPTQTPRDAGVGDALTPPVPPISDAGVRID
                     SGMSPVLQRDSGMRR"
BASE COUNT          313 a          759 c          736 g          275 t
ORIGIN      
        1 cagggaccac tgccgcggca ggtgccgagg cagctgccgg ggccgggatc gtgacgatca
       61 gcccggcggt cggctggatc ggctgtgccg gctcgaccgg cgtggcctgg gcggtccgtg
      121 tcggactggc actcgcggcg ggcaagcctt cgaggtgcgc ggacgcctcg gtgacgccgg
      181 gagccggcgg cggtcgcagc gtcgggtccg ccgggccggg cgcggtggac tggccgagct
      241 cggggccgaa catgggctcc gcggtgtcca tcggatgccc ggcgcatcca ccgtcgctgc
      301 aaatgaccac cgccgcgacc agcgcggcgc ggagagcgga gagcgtcatg ccaggtgacc
      361 ctgcgagccg cgtgcccgcg ccgtgcggcg tggcgcgctg cgacgaggga tccagcgcgt
      421 ggctcgctgc cgcggtggca cgctcgcgct gcgagatcct cgcgtgcgcg ccggcccggc
      481 gctggtatgc cgatcgcaac gcccgatggc atgcccgcgc gctccatcga tacggcgacc
      541 ctgacgttcg gcctggtcgc gatcccggtg cggatctacg cgaccagcga gcggtcccac
      601 gagatccact tccacctcgt gcacgagggc tgcggcgagc ggctccacca gcagtacgtc
      661 tgcccgcgtc acggtccggt cgaccgcgac gagatcatca agggctacga gctgagccgc
      721 ggcagcttcg tcgagctgtc gcgcccggag ctcgaggcgc tcgaggcggt ctcgtcggac
      781 gagatcgcga tccaggagtt cgtgccggcc gccgcggtcg acccgctgct gatcgagcac
      841 acctattacc tcgggcccgg caagggtggc gagcgggcct accggctgct gcgcgacgcg
      901 ctcgccgccg ccgacctggt cgggatcgcg agctacgccg cgcgcggcaa gcagtacatc
      961 gtcgagctgc tgccgtacga gaccgggctc gcgatgctcc agctgcgcta cgccgacgag
     1021 ctcaagccgt ggagcgaggt cccggcgccg ccgcaggtca agccgtccgc cgccgagatc
     1081 gcgctcgcca gcaagatcat cgacaacctg cggcgcgcga ccttcgatcc gtcgcgctac
     1141 cacgacgagg tcaaggaccg cgtgcgcgac ctgatcgcga gcaaggccaa gggcggcgag
     1201 atcgtcgcgc cgcccagcgc cgagcgaccg ccggtgaccg acctcatggc cgcgctcaag
     1261 gcctcgctgg cgaacggtga gtctcgaccg gagggtgcgc ggacccccgc ccgtcgctcc
     1321 gcgcagggcc gccgctcgaa cggagcgggt cgtgcgtcca gcgcgcaccg gtccagagcc
     1381 ccgcgcgtcg gcgcacaccg ctgaagaaat gtcgattcaa gcggaccgaa gccggttccg
     1441 gaatctccac ggccgatctg tgggggcccg gcggggaaac tcgccaggct acccgtcgac
     1501 ggcgacgtac gtcgacgatg acgtcgacct tgactccagc gttgagcttg acggcagcga
     1561 agcgatacgt agacgtctga ccccaccgtc gacctcgacc tcgcccccgt tgtcgccctc
     1621 cctcgaggat cgcagcacag gatcggtcga cgtccaacgt cgatggtggg ctcaacgtcg
     1681 tacgtcgccg tcgacctcaa cgctctgggt caacgatcag gtcgacgtcg acgatcacgg
     1741 acgccatcga ggacttgtga cgaatcatca gacggccaca tcaacctcga cgacggccgt
     1801 caaggtcaag gcccaggtcg acgtcaaccg ggcgatgcac ctgcggcctc cgagatcagc
     1861 gccaccggcg cagtgaccgc gcggcgccct atcctatctc gatcagcgcc gcatgccgga
     1921 gtcgcgctgc agcaccggcg acatgccgga atcgatccgc acgcccgcat ccgagatcgg
     1981 cggcaccggc ggggtgagcg catcgcccac gcctgcatcg cgcggcgtct gggtcggcgc
     2041 acccggcgcc tgcggtgccc ccggcggcgg cgtcggcgcg ccc
//
LOCUS       DASVOR010001321         7435 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_364267,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001321 DASVOR010000000
VERSION     DASVOR010001321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364267"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..573)
                     /locus_tag="VF516_25200"
     CDS             complement(94..573)
                     /locus_tag="VF516_25200"
                     /inference="COORDINATES: protein motif:HMM:NF002449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HEX8111061.1"
                     /translation="MIACPCGSGAALDACCGLWLDGVPAPTALALMRSRYTAYVRGAI
                     DYLIETHDPSTRSSIDRAGITDWSRQTEWLGLEILDTVRGGEDDDDGIVEFIARGRTR
                     GAPFAHRERSRFTRREGRWYYVDGTQPREPARAAAAVGRNDPCPCGSGMKYKRCHGA"
     gene            complement(588..1481)
                     /locus_tag="VF516_25205"
     CDS             complement(588..1481)
                     /locus_tag="VF516_25205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111062.1"
                     /translation="MKRTTYAVAALALSNVLGAGCAAGPDGDPSPESLGVSQDALSGL
                     IVASWGGVGTGGPTFSNGASIVLPLGVGNGWVCWLAGMRGTLGTLGNVGVFQADASGD
                     PADNLGPNEWALVVEPDTGNAAAGNAVCAPATNRGRFFGDALPETLLTSATVNDWCGL
                     SEINSFPFGPPHLPWSLASDFATVSNNGSNPQIVVGGSTWTFSGALSGTVDANCAHAS
                     VSGIWFYHIIAPSSGTASFNLLQNNGAQLPAGTACFLTNLKGAFVNNSFTDGVFINRT
                     SSGMWSLTASNGKSGWALCML"
     gene            1662..2117
                     /locus_tag="VF516_25210"
     CDS             1662..2117
                     /locus_tag="VF516_25210"
                     /inference="COORDINATES: protein motif:HMM:NF014082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8111063.1"
                     /translation="MFDLTPIGTVRCTRTTPEDDHWAGTTATIELAPGVPAESLDGLS
                     EFSHAEIVYVFDRVPPEKIVTGARHPRNNPAWPRVGIFAQRGKNRPNRLGVSVVRIVG
                     VDGAKLHVAELDAIDGTPVVDIKPVLREFLPRGELRQPAWASELMAHYW"
     gene            2272..3141
                     /gene="ttcA"
                     /locus_tag="VF516_25215"
     CDS             2272..3141
                     /gene="ttcA"
                     /locus_tag="VF516_25215"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF007972.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEX8111064.1"
                     /translation="MTEAARRLEHRLQSDVGATCRRYQLLAPGDRILVAMSGGKDSYT
                     LFHLLTRIVPRLPFRVELIAVHLDQVQPGYDGSGLRAYLEASGQPFEILREDTYSVVT
                     SRLPETATYCSLCSRLRRGILYTAAERLGANKIALGHHRDDSLETFLLNLFYSGKLQA
                     MPASYRTDDGRFEVIRPLIECAEDDIAQLAAALAFPIIPCNLCGSQDGLRRDAMTRLL
                     AELERDNPNVRAIMLNALRNVRPTHLLDREVARAWAERDPEIRPAVVAAPRPRHAAAE
                     PVVDGKRRLRVVD"
     gene            3268..3606
                     /locus_tag="VF516_25220"
     CDS             3268..3606
                     /locus_tag="VF516_25220"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEX8111065.1"
                     /translation="MTSRPHDALFKSAFEAPADAAALLRELLPVVLRELIAWDTLHGE
                     PASFVDPALADLHSDLLYSAQLRTDPPELLHVLLEHLCGAPHKCSYAEPPIMWSGPLP
                     RVGADAFRSA"
     gene            complement(3943..4602)
                     /locus_tag="VF516_25225"
     CDS             complement(3943..4602)
                     /locus_tag="VF516_25225"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8111066.1"
                     /translation="MTPLAPHLTAFLRERLPVHRRASQHTCDTYAYAFQLLLSFASQR
                     LGRPPSALGLEELDVPLISDFLVHLQTVRHNEPRTRNARLAAIKSFMHFLEHRVPSAM
                     EQIRRVLAIPAQRCDRRLVRHLAADEERVLLDAPDPTTRMGIRDRAMLHVALAGGLRV
                     SELIGIRMDDLRFDGPYVDVLAFAARGARSASSACGSASAIPFAPGSRFAATRPCPSS
                     S"
     gene            complement(4599..5510)
                     /locus_tag="VF516_25230"
     CDS             complement(4599..5510)
                     /locus_tag="VF516_25230"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8111067.1"
                     /translation="MLSQAVEDYIRLRRIGGFKFGVQALLVRSYARFATARGDSHVVT
                     QTVIDWAAKSPSPESREVRLQTVIAFARHCRAEDVKHEVPPAGVFGKTYSRRPIAYIF
                     SSEEIARLLAGALALQPKASLRPYTLHVIFGLLAATGLRLGEALRLRFEDLTPEGLLI
                     RETKFRKSRLVPLHPTTTQVLQRYVKLRTLLGGDDGHVFVGLNGQRLSSGTVQTIFRE
                     VATTIGLDAGPGKRLPRLHDLRHTWAVRALESSPREVGLIDRHMRAVTAYLGHAKVAS
                     GYWYLHATPFLMKRIADACAAREQEEP"
     gene            complement(5504..6757)
                     /locus_tag="VF516_25235"
     CDS             complement(5504..6757)
                     /locus_tag="VF516_25235"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8111068.1"
                     /translation="MFKELFPNSETRAWISSGMAASFVHSYAMSLRRAGFRPSTLRQQ
                     IRTAAHLGHWIESEGRALRDLDEDTLQRFREHLLTCTCHHSRPGEHHHTAIGSERFLK
                     HLRTVGVVAPRPEAPDPVGDLVTRFKSWLVRHRGVAPDTLTGYCSQVNAAIDPLGADP
                     RRYDVTSVRQFMIAHLGSYGTTQAKVAGYALRTFLRFLVAEGLCPPEIVDAVPRFANW
                     TLTELPRHLPPEAMEQVISTPDTCTRMGIRNRAVLLLLARLGLRARDIVELRRSDLDW
                     KRGLVRVMGKGRRETWLPLPQDAGDAVRDYLEKARPRSPHERLFLSVRAPFRPFKGTP
                     AVAFLVRQAIARAGVQRPAGVATHLFRHSLAKRLLAHDIPLEGIGVILRHRSLETSAQ
                     YAKIDVGTLQTVVQAWPGNPEVLPC"
     gene            6951..7409
                     /locus_tag="VF516_25240"
     CDS             6951..7409
                     /locus_tag="VF516_25240"
                     /inference="COORDINATES: protein motif:HMM:NF025619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HEX8111069.1"
                     /translation="MAERMLHYQVMCLKYARTRADFVRILGRWMDVAREVARAPHGLE
                     ALAQVMCYVLEVNEYVEPEELQGLLEREIGPEAKDAIMTAGQRLIEQGRQQGRQQGIQ
                     ELLLRLLRQRFGDEVDAQVEQRIATASFEEIDGWSSRVLSAATLAELFAD"
BASE COUNT         1187 a         2491 c         2598 g         1159 t
ORIGIN      
        1 ggcagatccg gggctgtcca ccccgtgacc cccgaggacg cccgccacat cgagctcgac
       61 ggcgcgtgcc tgcgcgcgtg atggcgagcg gcctcaggcc ccatggcacc gcttgtactt
      121 catcccgctg ccgcacggac acggatcgtt gcggccgacc gccgcggcgg cgcgcgccgg
      181 ctcgcgcggc tgcgtaccgt cgacgtagta ccagcgaccc tcgcggcgcg tgaaccgcga
      241 gcgctcgcgg tgggcgaacg gcgccccgcg cgtgcggccg cgggcgatga actcgacgat
      301 gccatcgtca tcgtcttcgc cgccgcgcac cgtgtcgagg atctcgagcc cgagccactc
      361 ggtctggcgc gaccagtcgg tgatcccggc gcgatcgatc gagctgcggg tcgacggatc
      421 gtgggtctcg atcaggtagt cgatcgcgcc gcggacgtac gcggtgtagc gcgagcgcat
      481 gagggcgagc gcggtcggcg ccggcacgcc gtcgagccac aggccgcagc aggcgtcgag
      541 cgcagctccc gatccgcacg gacaggcgat caccggggca ttctagatca cagcatgcag
      601 agcgcccagc cgctcttgcc gttgctcgcg gtcagactcc acatgccgct cgaggtacgg
      661 ttgatgaaca cgccgtccgt gaagctgttg ttcacgaacg cacccttcag gttggtgagg
      721 aagcacgcgg tgccggcggg cagctgcgcg ccattgttct ggagcaggtt gaagctggca
      781 gtccccgacg acggtgcgat gatatggtaa aaccagatcc ccgagacact ggcatgcgca
      841 cagttcgcgt cgaccgtgcc cgacagcgcg ccggagaacg tccaggtcga tccaccgacg
      901 acgatctgtg gattgctgcc gttgttggag accgtcgcga agtcgctcgc gaggctccac
      961 ggcaggtgcg gtggcccgaa cggaaacgag ttgatctcgg acaggccgca ccagtcattg
     1021 acggtggccg acgtcaggag cgtctccggc agggcgtccc cgaagaatct gccgcggttg
     1081 gtcgcgggcg cgcagaccgc gttccctgcg gcggcgttgc ccgtgtcggg ctcgacgacc
     1141 agcgcccact cgttgggtcc gaggttgtcc gccggatcgc ccgacgcgtc ggcctggaac
     1201 acgccgacgt tgccgagcgt ccccagcgtg ccgcgcatcc cggcgagcca gcacacccag
     1261 ccgttgccga ccccgagcgg caggacgatc gacgccccgt tgctgaacgt ggggccgccg
     1321 gtgccgacac cgccccagct ggcaacgatg agccccgaga gcgcatcctg gctgacgccg
     1381 agcgactcgg gcgagggatc gccgtcggga ccggcggcgc atcctgcacc caggacattc
     1441 gacagcgcga gcgcggcgac ggcgtacgtc gtccgcttca tgaatgcttt catgatcatc
     1501 acgacctcct gaacatggac aggtgacccg cggttgcggc acctcgcccg gatacagtgc
     1561 ggccaggggc ggttgtgacc ggctgtccgc ggcgggacga gcgagcaggg tttgcctcgc
     1621 cgggcggcca acgaagcggc gcgctcgggc atcgaacgtt catgttcgac ctcaccccga
     1681 tcggcaccgt ccggtgcacg cgcacgacgc ccgaggacga ccactgggcc ggcaccaccg
     1741 ccacgatcga gctcgcgccc ggggttcccg cggagagcct cgacggtctc tccgagttct
     1801 cgcacgcgga gatcgtctac gtgttcgatc gggtgccgcc cgagaagatc gtgaccggcg
     1861 ctcgccaccc gcggaacaac ccggcgtggc cgcgcgtcgg catcttcgcc cagcgcggca
     1921 agaaccggcc gaaccggctg ggcgtctcgg tcgtccgcat cgtcggggtc gacggcgcga
     1981 agctgcacgt cgccgagctc gacgcgatcg acggcacccc ggtggtcgac atcaagccgg
     2041 tcctccgcga gttcctgccg cgcggcgagc tgcgccagcc tgcgtgggcg agcgagctga
     2101 tggcgcatta ctggtagagc gttcgcgccg cggggccgga tgcggtgcga tatcgcgatc
     2161 gccgttgccc tgaccgccgc gacgccgtcc aggccgcgcg tcgatcctcg cggctttccg
     2221 tgacgggacg cccaagatcg ggtacgtatc cgggatcccg gagcgatgat gatgaccgag
     2281 gccgcgcgac ggctcgaaca ccgcctgcag agcgacgtcg gcgcgacctg ccggcgctac
     2341 cagctgctcg ctcccggtga ccggatcctg gtcgcgatgt cgggcggcaa ggacagctac
     2401 acgctgttcc acctgctgac ccggatcgtg ccgcggctgc cgttccgcgt cgagctcatc
     2461 gcggtgcacc tcgatcaggt ccagcccggg tacgacggca gcgggctgcg cgcctacctc
     2521 gaggcgtcgg gccagccgtt cgagatcctg cgcgaggata cctactcggt ggtgacgtcg
     2581 cggctgcccg agaccgcgac gtactgctcg ctgtgctcgc ggctgcgccg gggcatcctg
     2641 tacacggccg ccgagcggct cggcgccaac aagatcgcgc tggggcacca ccgtgacgac
     2701 tcgctggaga ccttcctgct caacctgttc tacagcggca agctgcaggc gatgccggcg
     2761 agctaccgca cggacgacgg gcggttcgag gtgatccggc cgctgatcga gtgcgccgag
     2821 gacgacatcg cacagctcgc cgccgcgctg gcgttcccga tcatcccgtg caacctgtgc
     2881 ggctcgcagg acgggctgcg gcgtgacgcg atgacccggc tgctggccga gctcgagcgc
     2941 gacaacccca acgtccgcgc gatcatgctg aacgccctgc gcaacgtgcg gccgacccac
     3001 ctgctcgatc gcgaggtcgc ccgggcatgg gccgagcgcg atccggagat ccgtccggcg
     3061 gtggtggccg cgccgcggcc gcgccatgcc gccgccgagc cagtggtcga cggcaagcgc
     3121 cggctgcgcg tggtcgactg acgggccgtg tccggggttt gtccgaattt cggacgaccc
     3181 cggccgccgg caggacactc cggacacgcg gcgccgtgat ttctggggga tgcgtcggcg
     3241 cgatgcttgc tggcgtcctc cgccgtcatg acctcgcgcc cgcacgatgc gttgttcaag
     3301 tcggcattcg aggctcccgc cgacgcggcg gcgctgctgc gcgagctact gcccgtcgtg
     3361 ctccgcgagc tcatcgcgtg ggacacgctg cacggcgaac ccgcgtcgtt cgtcgatccg
     3421 gccctcgccg accttcacag cgatctgctg tactccgcgc agctgcgaac cgatccgccg
     3481 gagctgttgc atgttctact cgagcattta tgcggtgcac cgcataagtg ctcttatgcg
     3541 gagccgccga ttatgtggag cggccctctg ccgcgcgtcg gggccgacgc gtttcgctcc
     3601 gcataatggg ttgcccgcgg aggctggcga tgaggcgatc aggtgggcgg aatttgcccg
     3661 gcctgagcgc cggcggaatt gcgccggcca cggcttgcag cttctccagt ggatcgagct
     3721 cgaggtagat ctcgctggtc gtcgtgctcg cgtgcccgag ccagagcgcc acctggcgga
     3781 tgtcaccggt ggccttgagg accctcaagg cgcaggtgtg cctcagcacg tgcggggaca
     3841 gcggtcgccc ggcgagcgat gggcaccggc tcgccgcgat cgccttacac cggtcgagga
     3901 cgtactcgaa tccggcgcgc gtcatgtggc ggccctgcgc atttacgaag agctcgggca
     3961 cggccgtgtc gccgcgaacc gcgagccagg cgcgaatgga attgccgacg cgcttccaca
     4021 ggcggagctg gcgctccttg cgccccttgc cgcgaacgcg aggacgtcga cgtaggggcc
     4081 gtcgaaacgc aggtcatcca tgcggatacc gatcagctcc gaaacgcgaa gcccgccggc
     4141 cagcgcgacg tgcagcatcg cacgatcccg aatgcccatc cgggtcgtgg gatcgggcgc
     4201 atcgagcagg acccgctcct catcggccgc caggtgtcgt acgagcctcc ggtcgcagcg
     4261 ctgggcgggg atcgcgagca cgcggcggat ctgctccatg gccgaaggga cgcgatgctc
     4321 gaggaagtgc atgaacgact tgatcgcggc gaggcgagcg tttcgcgtac gaggctcgtt
     4381 gtgtcggacg gtctggagat gcaccaggaa gtccgagatc aacgggacgt cgagctcctc
     4441 gaggccgagc gccgacggtg gcctgccgag cctctggctg gcgaagctga gcaggagctg
     4501 aaacgcgtag gcgtaggtat cgcaggtgtg ctggctcgct cggcggtgca ccggcagccg
     4561 ctcccggaga aatgccgtca ggtggggggc caggggcgtc atggctcctc ctgctctcgc
     4621 gcggcgcagg cgtcggcgat ccgtttcatc aagaacggcg tggcgtggag gtaccagtag
     4681 ccagaggcca ccttcgcgtg tccgaggtag gccgtgacgg cgcgcatgtg gcgatcgatc
     4741 aagccgacct cccggggact cgactcaaga gcccgcacgg cccaggtgtg gcggagatcg
     4801 tgcagtcgag ggaggcgttt gccagggcct gcatccagtc cgatggtcgt ggcgacctcg
     4861 cgaaagatgg tctggacggt cccggagctc aaccgctggc cgttgagccc gacgaagaca
     4921 tggccgtcgt cgcctccaag gagggtgcgc agcttgacgt acctctgcag gacttgggtc
     4981 gtcgtcgggt ggagaggcac cagccggctc ttgcggaact tggtctctcg aatgagcagt
     5041 ccctccggcg tgaggtcctc gaaccgcaac cgcagggcct ctccgagtcg aaggccggtc
     5101 gcggcgagca agccgaagat cacgtggagc gtgtacgggc gcagcgaagc cttgggctgt
     5161 aacgccagcg ctcccgcgag gaggcgcgcg atctcctcgg acgagaagat gtacgcgatg
     5221 gggcggcgcg agtacgtctt gccgaagaca cctgctggcg ggacctcgtg tttcacatcc
     5281 tcggcgcggc agtggcgtgc aaaggcgatg actgtttgca gccgtacctc cctggactcg
     5341 ggcgaagggc tcttcgccgc ccagtcgatc accgtctggg tcacgacgtg agagtcccct
     5401 cgtgccgtcg cgaagcgagc gtacgagcgc accaggagcg cctgaacgcc gaacttgaat
     5461 ccgccgatcc gcctcagccg gatgtagtcc tcgaccgcct ggctcagcat ggcagcacct
     5521 ctggattccc cggccacgcc tgcaccacgg tctggagcgt ccccacgtcg atcttggcgt
     5581 actgcgcgct ggtctcgagg ctgcgatggc ggaggatgac gccgatgccc tcgagcggga
     5641 tgtcgtgcgc gagcagtcgc ttcgcgaggg aatgccggaa caggtgcgtg gcgacccctg
     5701 ccggccgctg aactcctgct cgagcaatgg cctgacgcac gaggaaggcc acggcggggg
     5761 tgcccttgaa cggacggaac ggagcgcgaa cggacaggaa caggcgctca tgtggagagc
     5821 gcggacgcgc cttctccagg tagtcccgta ccgcatcccc tgcgtcctgc ggcagcggta
     5881 gccaggtctc gcggcgtcct ttgcccatca cgcgcaccag ccctcgcttc cagtcgagat
     5941 cggacctccg tagctcgacg atgtcgcggg ccctcaggcc gagccgggcg agcagcaaga
     6001 gcaccgctcg gtttcgaatt cccatccgcg tgcatgtatc cggcgtggag atcacctgct
     6061 ccattgcttc gggcggtaga tgccgcggca gctccgtgag ggtccaattg gcgaacctcg
     6121 ggaccgcatc gacgatctcc ggtgggcaca acccctctgc gacgaggaac cgcaggaacg
     6181 tccgcagcgc gtagcccgcg accttggcct gggtggtccc gtagctgccc aggtgcgcga
     6241 tcatgaactg ccgcacggag gtgacgtcgt agcggcgtgg atccgctccg agcggatcga
     6301 tggctgcatt gacctgcgag caatagccgg tcagcgtgtc cggggcgacg ccgcgatgac
     6361 ggaccagcca gctcttgaac cgcgtcacca aatcaccgac cggatccggt gcctcgggcc
     6421 gaggtgccac gacaccgacg gtgcggagat gcttgaggaa ccgctccgat ccgatggctg
     6481 tgtgatggtg ctcgcccggt cgcgaatggt ggcaggtgca agtcagaagg tgctcgcgaa
     6541 agcgttgcag ggtgtcctcg tcaagatcac ggagcgcgcg cccttcgctc tcgatccagt
     6601 ggcccagatg cgcagcggta cggatctgct ggcgcagcgt gctgggcctg aagccggccc
     6661 gacgcagcga catcgcgtac gagtgcacga acgacgccgc catccctgaa ctgatccatg
     6721 cgcgcgtctc ggaattcgga aagagctcct tgaacatgat gacctcctcg gggacttccc
     6781 cggaggtgta aggtcaccag cctcctgccg ttatgcgaag tgggcacggc ggcggcggcg
     6841 gcgcaacgtg ctgcaaaaca cgcgagcctg aagcacctcg gagggcacag ctccgcatag
     6901 tcggcggctc cgcataagag catcaggcgg cacccccgac cgagggccgg atggccgagc
     6961 gcatgctcca ctatcaggtg atgtgcttga agtacgcgcg gacgcgcgcg gacttcgttc
     7021 ggatcctcgg ccgctggatg gacgtcgcgc gcgaggtggc ccgggctccg cacgggttgg
     7081 aggcgctggc gcaggtgatg tgctatgttc ttgaggtcaa cgagtacgtc gaacccgagg
     7141 agctgcaagg acttttggaa cgcgagatcg gacctgaagc caaggacgct atcatgaccg
     7201 ccggacagcg actcatcgaa caaggacgcc agcaagggcg ccagcagggc atccaagagt
     7261 tgttgctgcg cttgctgcgg cagcgcttcg gcgatgaggt ggacgcccag gtcgagcagc
     7321 gcatcgcgac ggcctccttc gaggagatcg atggctggtc ctcgcgcgtg ctatcggcag
     7381 ctacgctcgc cgagttgttc gccgattgaa ccggaaggct gaccagcgtc gcgcc
//
LOCUS       DASVOR010001322         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_422034,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001322 DASVOR010000000
VERSION     DASVOR010001322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422034"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /gene="pnp"
                     /locus_tag="VF516_25245"
     CDS             <1..1742
                     /gene="pnp"
                     /locus_tag="VF516_25245"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX8111070.1"
                     /translation="MTGASAALHCSDLVWSGPLAGVRIARIDGEFVANPTFAEREKSE
                     MDIVVAASKDAIMMVEGEMHEMQENVVIDALLFAHQAVQPLIELQERLRAANGKDKRP
                     FTPPAADEALAAQVKELAWGRLGEAMTIKDKHKRRTAVSTLHLEVRGALTTEGQPWAG
                     RSKEVDGAFAKLEKKWARGHTLATRNRIDGRAPDEIRAISIETGVLPRAHGSALFTRG
                     ETQALVAVTLGTKYDEKKLDTLLGDRKKTFYMDYNFPPFSTGEVKPLRGQSRREVGHG
                     FLAERSVEAVVPVYEDFPYTIRVVSDILESNGSSSMASVCGGSLALMDAGVPTKAPIA
                     GIAMGLMKEGNDYVVLSDILGDEDHLGDMDFKVTGTRRGVCALQMDIKVDGLTREILE
                     KALDQAKRGRIFILDKMDRAISAPRDEVSVYAPRIVTVQIKPDKVRDIIGPGGKTIRA
                     IQEQTGAQIDIADNGVVSIASSNSKAIEQAQALISGLTMEPEVGQFYNGIVKKIVEIG
                     AFVEIMPGTDGLLHISEVAKERIRAVEDVLNEGDEVIVKCIKVDRDGKIRLSRREALD
                     ANPAPEEVHNYII"
     gene            1777..2217
                     /gene="dut"
                     /locus_tag="VF516_25250"
     CDS             1777..2217
                     /gene="dut"
                     /locus_tag="VF516_25250"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEX8111071.1"
                     /translation="MAPRVLIQKLRADAIVPRYMTAHAAGLDLSAALDQPLEIAAGQR
                     AAIPTGLAIKLPDGYEAQVRPRSGLARDHGVTLVNSPGTIDCDYTGELVVLVINHGDR
                     AVRIEPGQRIAQLVVAPVVQAELVEVSELPATARGAGGFGSTGR"
     gene            2214..>2489
                     /locus_tag="VF516_25255"
     CDS             2214..>2489
                     /locus_tag="VF516_25255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111072.1"
                     /translation="MTVRSKRSTQEGTGVSHPERRRALLGTPPHGAATRGESWELVTE
                     VTPRPDLLLTISLVGAMFCGEGGAALVGFSGALLHASLAAPPHAGVGS"
BASE COUNT          409 a          857 c          891 g          332 t
ORIGIN      
        1 cgatgacggg cgcgtccgcc gcgcttcact gcagcgacct cgtgtggtcg ggcccgctcg
       61 ccggcgtccg catcgcgcgc atcgacggcg agttcgtcgc gaacccgacg ttcgccgagc
      121 gcgagaagtc ggagatggac atcgtggtcg cggccagcaa ggacgcgatc atgatggtcg
      181 agggcgagat gcacgagatg caggagaacg tcgtgatcga cgcgctcctg ttcgcgcacc
      241 aggcggtcca gccgctgatc gagctccagg agcggctgcg ggcggccaac ggcaaggaca
      301 agcgaccgtt cactccgccg gcggccgacg aggcgctcgc cgcgcaggtc aaggagctcg
      361 cgtggggccg gctcggcgag gcgatgacga tcaaggacaa gcacaagcgc cgcaccgcgg
      421 tgtcgacgct gcacctcgag gtccgcggcg cgctgaccac cgagggccag ccgtgggccg
      481 gccggtccaa ggaggtcgac ggcgcgttcg ccaagctgga gaagaagtgg gcgcgcggcc
      541 acacgctggc gacccgcaac cggatcgacg gccgcgcgcc cgacgagatc cgcgcgatct
      601 cgatcgagac cggcgtgctg ccgcgcgcgc acggctcggc gctgttcacc cgcggcgaga
      661 cccaggcgct ggtcgcggtc acgctgggca ccaagtacga cgagaagaag ctcgacaccc
      721 tgctcggcga ccggaagaag acgttctaca tggactacaa cttcccgccg ttctcgaccg
      781 gcgaggtcaa gccgctgcgc ggccagtcgc gccgcgaggt cggccacggc ttcctcgccg
      841 agcgcagcgt cgaggcggtg gtgccggtgt acgaggactt cccgtacacg atccgcgtgg
      901 tgtccgacat cctcgagtcc aacggctcgt cgtcgatggc gtcggtgtgc ggcggctcgc
      961 tcgcgctgat ggacgccggc gtgccgacca aggcgccgat cgcgggcatc gcgatgggcc
     1021 tgatgaagga gggcaacgac tacgtcgtgc tgtccgacat cctcggcgac gaggaccacc
     1081 tcggcgacat ggacttcaag gtcaccggca cgcggcgcgg cgtgtgcgcg ctgcagatgg
     1141 acatcaaggt cgacggcctg acccgcgaga tcctcgagaa ggcgctcgac caggccaagc
     1201 gcggccggat cttcatcctc gacaagatgg accgcgcgat ctcggcgccg cgcgacgagg
     1261 tctcggtcta cgcgccgcgc atcgtcacgg tgcagatcaa gccggacaag gtccgcgaca
     1321 tcatcggccc cggcggcaag acgatccgcg cgatccagga gcagaccggc gcccagatcg
     1381 acatcgccga caacggcgtg gtgtcgatcg cgtcgtccaa ctccaaggcg atcgagcagg
     1441 cccaggccct gatctcgggc ctgacgatgg agcccgaggt cggccagttc tacaacggca
     1501 tcgtcaagaa gatcgtcgag atcggcgcgt tcgtcgagat catgccgggg accgacggcc
     1561 tgctccacat ctccgaggtc gccaaggagc ggatccgcgc ggtcgaggac gtgctcaacg
     1621 agggcgacga ggtgatcgtc aagtgcatca aggtcgaccg cgacggcaag atccgcctct
     1681 cgcggcgcga ggcgctcgac gccaacccgg cgcccgaaga ggtccataac tacatcatct
     1741 gaaccggccg ccgggttttc ggctacgatg gagcgcgtgg caccgcgcgt gctgatccag
     1801 aagctgcgag ccgacgcgat cgtcccgcgc tacatgacgg cccacgccgc cgggctcgat
     1861 ctcagcgcag cgctcgacca gccgctcgag atcgccgccg gccagcgcgc tgcgatcccg
     1921 accgggctgg cgatcaagct gccggacggc tacgaggcgc aggtccggcc gcgctccgga
     1981 ctcgcgcgcg atcacggcgt cacgctggtc aactcgcccg ggacgatcga ttgcgactac
     2041 accggtgagc tcgtcgtgct ggtcatcaac cacggcgacc gcgcggtccg gatcgagccg
     2101 gggcagcgga tcgcccagct cgtggtcgcg ccggtcgtcc aggccgagct cgtcgaggtc
     2161 agcgagctgc cggccacggc gcgcggcgcc ggtggcttcg gatcgacggg gcgatgaccg
     2221 tgcggtcgaa gcgatccacg caggagggca ccggggtgtc ccaccccgag cgccggcgcg
     2281 cgctgctcgg cacgccgccc cacggcgcgg cgacccgggg cgagtcgtgg gagctcgtca
     2341 ccgaggtgac gccgcggccg gatctgctct tgacgatatc cctcgtgggc gcgatgttct
     2401 gcggcgaagg aggggcagcg cttgtgggct tctcgggggc gctcctgcat gcgagtctcg
     2461 ccgcgccgcc ccatgcgggc gtcggcagt
//
LOCUS       DASVOR010001323         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_130328,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001323 DASVOR010000000
VERSION     DASVOR010001323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130328"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VF516_25260"
     CDS             complement(<1..545)
                     /locus_tag="VF516_25260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111073.1"
                     /translation="MSSEPARAPSVHRPAEPGRPARGGARSPHTGHTGLAGSALVAVA
                     AASWGTWSLFLRPTGLPATVTAPIIFGVIGLVTLPVALRAPRVAWDRTTVGLVAANAA
                     FDALNIVAYFSAIRLTTIEIAVLTHYVAPILIALAAPRIDGVVTPGAGVAAGVALTGL
                     VVILEPWHAPADGAVLGAALGL"
     gene            657..1412
                     /locus_tag="VF516_25265"
     CDS             657..1412
                     /locus_tag="VF516_25265"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEX8111074.1"
                     /translation="MSDVSAVATAPKNLRTLAIDIGGTGLKALVLGADGKALTERARI
                     ETPHPATPSALVPAIVKLVEPLGAFDRISIGFPGVVVDGVTLTAPNLHKQWRGFDLAR
                     AMTERLHRPVRVLNDAGVQGYGVVEGRGVEMVLTLGTGLGCALYHEGRYVPNLELAHH
                     PFRGDKTYEDYVGAKALHRVGKKKWNRRVARVVEQIQPIWNPRWIYLGGGNAKHLSIE
                     LPSNITITPNIAGLMGGIVLWHDGPPAAAKPTR"
     gene            complement(1402..>1663)
                     /locus_tag="VF516_25270"
     CDS             complement(1402..>1663)
                     /locus_tag="VF516_25270"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HEX8111075.1"
                     /translation="GLQSYFTAGEKEIRAWTIRKGALAPEAAGVIHTDFEKGFIRAQV
                     YSVDDLEQHGNQAAIKAAGKLRVEGKDYVVHDGDVMHFLFNV"
BASE COUNT          255 a          592 c          612 g          204 t
ORIGIN      
        1 agcccgagcg ccgcgccgag caccgcgccg tcggccgggg cgtgccaggg ctcgaggatc
       61 acgaccaggc cggtgagcgc gacccccgcg gcgaccccgg cgcccggggt gaccacgccg
      121 tcgatccggg gcgcggcgag cgcgatcagg atcggcgcga cgtagtgggt caggacggcg
      181 atctcgatgg tggtgagccg gatcgcggag aagtacgcga cgatgttgag cgcgtcgaac
      241 gccgcgttgg cggcgaccag tccgaccgtc gtgcggtccc acgccacgcg cggggcgcgc
      301 agcgcgaccg gcagggtgac caggccgatc accccgaaga tgatcggcgc ggtgacggtc
      361 gccggcaggc cggtggggcg caggaacagg ctccaggtcc cccacgacgc ggcggcgacc
      421 gcgaccagcg cggacccggc gagcccggta tgcccggtat gtggagaccg tgcgccgccg
      481 cgcgccggcc ggcccggctc ggcaggtcga tggacgctcg gggcgcgcgc cggctccgac
      541 gacaagccga cagcctaccg tgacgcgacg cgggcgccag cagcggacgg tgccgaccgc
      601 ggggatctcg tcaccggcca gctgggctat cctgggtccg tacctccggg agggccatgt
      661 cggacgtcag cgcggtcgcg accgcaccca agaacctacg caccctcgcc atcgacatcg
      721 gcggcaccgg gctcaaggcg ctcgtgctcg gcgccgacgg caaggcgctg accgagcggg
      781 cgcggatcga gacgccgcac ccggcgaccc cgtcggcgct ggtccccgcg atcgtcaagc
      841 tggtcgagcc gctcggcgcg ttcgaccgga tcagcatcgg gttccccggc gtggtggtcg
      901 acggcgtcac gctgaccgcg cccaacctcc acaagcagtg gcggggcttc gatctcgccc
      961 gggcgatgac cgagcggctc caccggccgg tccgcgtgct caacgacgcc ggcgtccagg
     1021 gctacggcgt ggtcgagggc cgcggcgtcg agatggtgct gacgctcggc acgggcctgg
     1081 gctgcgcgct ctaccacgag ggccgctacg tccccaacct ggagctcgcg caccacccgt
     1141 tccgcggcga caagacctac gaggactacg tcggcgccaa ggcgctgcac cgggtcggca
     1201 agaagaagtg gaaccggcgg gtcgccaggg tcgtcgagca gatccagccg atctggaacc
     1261 cgcgctggat ctacctcggc ggcggcaacg ccaagcacct gtcgatcgag ctgccgagca
     1321 acatcacgat cacgcccaac atcgcgggcc tgatgggcgg catcgtgctg tggcacgacg
     1381 gaccgccggc cgcggcaaag cctacacgtt gaacaggaag tgcatgacgt cgccgtcgtg
     1441 gacgacgtag tccttgccct cgacgcgcag cttgccggcg gccttgatcg cggcctggtt
     1501 gccatgctgc tcgagatcat cgaccgagta gacctgggcg cggatgaagc ccttctcgaa
     1561 gtcggtgtgg atcacgccgg ccgcctcggg agcgagcgcg cccttgcgga tcgtccaggc
     1621 gcggatctcc ttctcgccgg cggtgaagta cgactgcagg ccg
//
LOCUS       DASVOR010001324         2656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43105,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001324 DASVOR010000000
VERSION     DASVOR010001324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2656
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43105"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2656
                     /locus_tag="VF516_25275"
     CDS             <1..>2656
                     /locus_tag="VF516_25275"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8111076.1"
                     /translation="DRLAAQHAAEEDAEAARHLIDLPRPMQIRRPPGGKLAESSALLA
                     RLYAGDRVPREKKPPVIDHLRSVGPWMVSVDAQPLVVLDGMSQTATVVGGFAEDPVVR
                     AYVEGELADTLVHNDDTALGETWAAAEYAQALRQLVPGLPHVTFVASGAEANEKAMAL
                     CRINCPRRGATRVLAFDGSFHGRTLLALHATHSPSKRAPFELPGYACEFAPFPLWNTP
                     GDEPAAPSGFYAAAATGDVAELRSRFGDAKDDPLLAAEVASLAAVDAALATGEYFCTI
                     IEPMQSEGGDRYATERFFRALRLLTRFHQTFLVFDEVQVGFGLGGPFAWHSRFRLLNA
                     RGQPDYPDAVTFAKRAQVGVVMSRFADPEPASAHGASLVRGRIHADMVSTAHNAERIE
                     KLVQPRLAQIARAYPHLVGHPRATGYAFAFDLPTPADHDAFIGQRFWRGAVVFGAGTR
                     TARYRLNDHFHAREIDLLFETIRRSLSWLDAHPGKKAPEWEDGVDVGEARPAAAAKLP
                     ELRYRSVPHSEAMDFLPAILDIEYQVYEPARRTPPAEIRAAIADAEGALLVVESPAPG
                     DGGHWQLVGFAIGAPLEDCKDVEGPDDDPMLGKHNTLYSVSITVAPGFQSAGIGRRLK
                     ELQLRDAQARKNPDGSPRYRYVSSRNRVGRTAEMTHLNRTFGAHVVSVLTGQYEDPEG
                     QAIYYRIPLGPIAPDPVVQQDVLRRRAASHPPGDLTVDLSSRLSSRAGAGQGVAVDPA
                     FDLASGLTRPFASGPASLREAEERGLLYGAAVNKITLMNYVTPATVRALEWIGALVPE
                     LPHMYLTSSRDEAVDKALRLIRCTRKTAQVAIGLLGGYYGHTAASCRSLSDPDVHLGG
                     PPHFAWPRVPHPAQAGTAATIDALRAAVG"
BASE COUNT          347 a         1015 c          953 g          341 t
ORIGIN      
        1 gaccggctgg cggcgcagca cgccgcggag gaggacgccg aggccgcgcg ccacctgatc
       61 gacctgccgc ggccgatgca gatccgccgg ccgccgggcg gcaagctcgc cgagtcgtcg
      121 gcgctgctcg cccggctcta cgccggcgac cgggtgccgc gcgagaagaa gccgccggtg
      181 atcgaccacc tgcgctcggt cgggccgtgg atggtgtcgg tcgacgccca gccgctggtc
      241 gtgctcgacg ggatgagcca gaccgcgacc gtggtcggcg ggttcgccga ggatccggtg
      301 gtgcgggcct acgtcgaggg cgagctcgcc gacaccctgg tccacaacga cgacaccgcg
      361 ctgggcgaga cctgggcggc cgccgagtac gcacaggcct tgcgccagct cgtgcccggc
      421 ctgccgcacg tcacgttcgt ggcgtcgggc gccgaggcca acgagaaggc gatggcgctg
      481 tgccggatca actgcccgcg ccgcggcgcg acccgggtgc tggcgttcga cggcagcttc
      541 cacggccgca ccctgctcgc gctgcacgcg acccactcgc cgagcaagcg cgcgccgttc
      601 gagctgcctg ggtatgcgtg cgagttcgcg ccgttcccgt tgtggaacac gccgggcgac
      661 gagccggccg cgccgagcgg gttctacgcc gcggccgcga ccggcgacgt cgccgagctg
      721 cgcagccggt tcggcgacgc caaggacgat ccgctgctcg ccgccgaggt cgcgtcgctc
      781 gccgcggtcg atgcggcgct cgcgaccggc gagtacttct gcacgatcat cgagccgatg
      841 cagagcgagg gcggcgaccg ctacgcgacc gagcggttct tccgcgcgct gcgcctcttg
      901 acccggttcc accagacctt cctggtgttc gacgaggtcc aggtcgggtt cgggctcggc
      961 gggccgttcg cctggcactc gcggttccgg ctgctcaacg cgcgcggcca gcccgactac
     1021 cccgacgcgg tgacgttcgc caagcgcgcc caggtcggcg tcgtgatgtc gcggttcgcc
     1081 gaccccgagc cggcgagcgc gcacggcgcg tcgctggtcc gcggccggat ccacgccgac
     1141 atggtgtcga ccgcgcacaa cgccgagcgg atcgagaagc tggtccagcc ccggctcgcc
     1201 cagatcgcgc gcgcctatcc ccacctggtc ggccacccgc gcgccaccgg ctacgcgttc
     1261 gcgttcgacc tgccgacgcc cgccgaccac gacgcgttca tcggccagcg gttctggcgc
     1321 ggcgcggtgg tgttcggcgc cggcacccgc accgcgcgct accggctcaa cgaccacttc
     1381 cacgcccgcg agatcgacct cctgttcgag accatccggc gctcgctgtc gtggctcgac
     1441 gcccacccgg gcaagaaggc gccggagtgg gaagatgggg tggacgtcgg cgaggcgcgc
     1501 ccggccgcgg ccgccaagct gcccgagctg cgctaccgct cggtgccgca ctccgaggcg
     1561 atggacttcc tgccggcgat cctcgacatc gagtaccagg tctacgagcc ggcgcggcgg
     1621 accccgccgg ccgagatccg cgccgcgatc gccgacgccg aaggcgcgct gctggtggtc
     1681 gagagcccgg cgcccggcga cggcggccac tggcagctgg tcgggttcgc gatcggcgcg
     1741 ccgctcgagg actgcaagga cgtcgagggc cccgacgacg atccgatgct cggcaagcac
     1801 aacacgctgt actcggtgtc gatcaccgtg gcgcccgggt tccagagcgc cggcatcggc
     1861 agaaggctca aggagctcca gctgcgcgac gcccaggcgc gcaagaaccc cgacggctcg
     1921 ccgcgctacc gctacgtgtc gagccgcaac cgggtcggcc gcaccgccga gatgacccac
     1981 ctcaaccgga cgttcggcgc gcacgtggtg tcggtgctga ccggccagta cgaggacccc
     2041 gagggccagg cgatctacta ccggatcccg ctcgggccga tcgcgcccga tccggtggtc
     2101 cagcaggacg tgctgcgccg gcgcgccgcg tcgcacccgc cgggcgacct gacggtcgat
     2161 ctctcgtccc ggctgtcgtc ccgggccggc gccggccagg gcgtcgcggt cgaccccgca
     2221 ttcgatctcg cctccggact gacccggccg ttcgcgtcgg ggcccgcgtc gctgcgcgag
     2281 gccgaggagc gcggcctcct gtacggcgcg gcggtcaaca agatcacgct gatgaactac
     2341 gtcacgccgg ccaccgtgcg cgcgctggag tggatcggcg cgctggtccc cgagctgccg
     2401 cacatgtacc tgacgtcgag ccgcgacgag gcggtcgaca aggcgctgcg cctgatccgc
     2461 tgcacccgca agaccgcgca ggtcgcgatc ggcctgctcg gcggctacta cggccacacc
     2521 gcggcgagct gccgctcgct gtcggatccc gacgtccacc tcggcggccc gccgcacttc
     2581 gcgtggccgc gcgtcccgca tccggcgcag gccgggaccg ccgcgacgat cgacgcgctg
     2641 cgcgccgcgg tcggcg
//
LOCUS       DASVOR010001325         7664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_71928,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001325 DASVOR010000000
VERSION     DASVOR010001325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71928"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VF516_25280"
     CDS             complement(<1..447)
                     /locus_tag="VF516_25280"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8111077.1"
                     /translation="MTADAAATSARPAATTDASAAPLLELTAVRAGYGDFQALFDISL
                     EVRAGEIVTLIGANGAGKTTTLRVISGAVRATSGTVRFDGHDVTGLPAHAMPERGISH
                     VPEARQLFPFMTVEENLELGAYNRRSRPRARDTRDEMLELFPRLKER"
     gene            complement(444..1181)
                     /locus_tag="VF516_25285"
     CDS             complement(444..1181)
                     /locus_tag="VF516_25285"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111078.1"
                     /translation="MTALLTLEHVSKRFGGLRAVNDVSLTITEGEVLFIVGPNGAGKT
                     TLFNLITGFLPPDEGVIRFAGRSLAGTPPHEAARLGIGRTFQIVKPLRNLTVLENVML
                     GAFLQTRDVAEAAARAREVLEFLQMGSVIAQPAAGLPLATLKRLEIARALATRPRLIL
                     LDEVVAGLPTTEALALAGLLQRLPEWGVAAVAGVEHVMQVVMKVAHRVVVLDRGALIA
                     EGTPADVVRRPEVIEAYLGAKYKQVTG"
     gene            complement(1178..2110)
                     /locus_tag="VF516_25290"
     CDS             complement(1178..2110)
                     /locus_tag="VF516_25290"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8111079.1"
                     /translation="MTRRRALLAAAGFGLCLLVPLFQGDYHVHVLFHVALFAALGLAW
                     NLVGGFAGQLSLGHAAFFGIGAYALPLYHDKLGIPLPLTLVLLIVTAVAAAAVIGRVA
                     FRLRGPYFALATIAFAEVLRLSAKNLDNLTGGDVGLQVPALFGPSAPIRWFYVSAVLL
                     TAAAFTATALIVRSRFGYQLQAVREDEDTARACGIDAARVKLHAFMISAAFTALGGAL
                     YMSQIASVVPDSAFAIDLSERPAIDAMLGGAGTLTGPIVGAILIETAQELFKNWFHEA
                     FQLIYGVLFVLVVLFLPEGIVGTIARWRRRQRAS"
     gene            complement(2107..2988)
                     /locus_tag="VF516_25295"
     CDS             complement(2107..2988)
                     /locus_tag="VF516_25295"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8111080.1"
                     /translation="MATQFWKALQAVLDGLLTGSLYSLIGMGMALIFGVMRIVNFAHG
                     AFMMIGMYVTYLLFDRLGVTPYVGFLAAAAVLFVLGAATYHGLLRRIAERSEFMTILL
                     TLGIAQMMIGVAQLAFSGDFRKANSALTTQLLRLGPHLTLNHGDLLSFAISAALASGM
                     FLFVMKSRFGRALRAIAQNRYAASLIGINVQRTQAIAFGLGIAAVGLAGGLLLPALYL
                     YPEVGAGYNLKSFVMVVLGGMGSIEGAAIAGLVLGVIENLTSLYWGNQWAAIVDFVIF
                     LLVLSLKPAGLFGSQRV"
     gene            complement(3013..4233)
                     /locus_tag="VF516_25300"
     CDS             complement(3013..4233)
                     /locus_tag="VF516_25300"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8111081.1"
                     /translation="MRSRWLPHAVVLASLFGVPAAACKGGGGGGKQEAGKTLRIGVIT
                     SLTGAQAAFGKAHKLGYEIAASEINAKGGVLGRQIEIIYYDDEGKPDQAAQGTTKLID
                     QDNVPIILGAYSSETTLAVIRAVTPKSVPLIMPTATADNTVETGSPWVFRICAGAKAY
                     AAAMADFLKNNGAPKTVAIIYENTNFGQSNGKAMEAAAPAAGMTVVANEAYQAKETDY
                     KALLQRVKDKDPEVVYYASYLLDAITLMKQTAQVDLNPKFLTAAGTGFSAAEFPSEDK
                     GAGKYAEYTFSVSQWLPSAKWEGSKQFDEAYFKLASTHPAYHGMQAYTALLVAADAIK
                     NAGSEDHQKIADALRKEHLDTPFGPIAFDAKGQNAHPVLITQVQGGKYKVVWPPDQAE
                     AKPILTTPKWSERQ"
     gene            complement(4446..5810)
                     /gene="aceA"
                     /locus_tag="VF516_25305"
     CDS             complement(4446..5810)
                     /gene="aceA"
                     /locus_tag="VF516_25305"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEX8111082.1"
                     /translation="MTIHIFNSYPGRDHRRTNEETAMTTSLSDHSALHAKRFDGIVRP
                     YTKADVDRLRGTYAIEHTVARLGARRLWELLHAEDYVAALGALTGNMAVQQVRAGLKA
                     IYLSGWQVAADANTAGQMYPDQSLYPVDSVPSVVRRINAALLRADQIDHAENKHERHW
                     LAPIVADAEAGFGGPLNAFELMKGMIDAGAAGVHFEDQLASEKKCGHMGGKVLVPTSH
                     FIRTLVAARLAADVSGVPTLVVARTDADSAKLLTSDIDERDRPFVAKGERTAEGFYRM
                     VSGIETAIARGLAYAPYADLVWCETSTPNLAQAKQFAEGIHRQFPNKLLAYNCSPSFN
                     WKKNLDDATIARFQRELAAMGYKFQFVTLAGFHALNYGMYELARKYKTGGMAAYSELQ
                     QAEFAAEANGYTATRHQREVGTGYFDQVAELISGGGASTLALHGSTEEAQFTPDPVRA
                     VAQR"
     gene            5913..7364
                     /locus_tag="VF516_25310"
     CDS             5913..7364
                     /locus_tag="VF516_25310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain fatty acyl-CoA regulator family
                     protein"
                     /protein_id="HEX8111083.1"
                     /translation="MERVTEQRLGAKVRGLRRREGLTQVQLATQLGISASYLNLIEAN
                     RRPLPANLLIKLAQLFGVDVAAFASDDDSRLVADLTEAFADPVLEGYDLTSVDVRELA
                     AASPEAARAVLGLYRAYQATRATADDLSSRLRDGEALPAPDGTRAPSLIPSEEVSDYL
                     QRASNYFPELEAATEELAVRARLTGDDLYAGLIRHLDQHLGVSVQIGHWEAMKGVLRR
                     YDPERRTLTLSELLPTRSRTFQLAHQIAIVSLGDVLDRHVAEGRFASTETRVLAKVAL
                     ANYFAGAVLMPYAAFHQAARSERYDIDVLGRRFRVGFEQVCHRLTTLRRPGAEGVPFH
                     MIRLDVAGNISKRFSASGIRFARFSGVCPRWNVFSAFLTPGMIRIQVSRMPDGQTYFC
                     IARTVQKDAGGFHAQQPVQSIGLGCQIEHARQLVYADGVNLESRDTLVLVGTTCRLCE
                     RTDCEQRAVPSLKVPLQIDENQRGMSLYTAVDR"
BASE COUNT         1126 a         2597 c         2774 g         1167 t
ORIGIN      
        1 gcgctccttt agccttggga acagctcgag catctcgtcg cgggtgtcgc gcgcccgcgg
       61 gcggctgcgc cggttgtagg cgccgagctc gaggttctcc tcgacggtca tgaacgggaa
      121 cagctggcgg gcctcgggga cgtggctgat cccgcgctcg ggcatcgcat gggcgggcag
      181 gccggtgacg tcgtggccgt cgaaccggac cgtaccgctg gtggcgcgca cggcacccga
      241 gatcacccgc agggtggtgg tcttgccggc gccattggcg ccgatcagcg tgacgatctc
      301 gccggcgcgg acctccaggc tgatatcgaa cagggcctgg aagtcgccgt agccggcgcg
      361 gacggcggtc agctcgagca gcggcgcggc ggacgcgtcg gtcgtggccg cgggccgggc
      421 ggacgtggca gcggcatcgg ccgtcatccc gtcacctgct tgtacttggc gccgaggtag
      481 gcctcgatca cctcggggcg gcggacgacg tcggcggggg tgccctcggc gatcagcgcg
      541 ccgcggtcga gcacgaccac gcggtgggcg accttcatga cgacctgcat gacgtgctcg
      601 acgcccgcga ccgcggccac gccccactcg ggcaggcgct gcagcaggcc ggcgagcgcg
      661 agcgcctcgg tggtcggcag gccggcgacc acctcgtcga gcaggatcag ccgggggcgg
      721 gtggcgagcg cgcgggcgat ctccaggcgc ttgagggtgg cgagcggcag gccggcggcc
      781 ggctgggcga tcaccgatcc catctggagg aactcgagga cctcgcgggc gcgcgcggcg
      841 gcctcggcga cgtcgcgggt ctggaggaac gcgccgagca tgacgttctc cagcacggtg
      901 aggttgcgca gcggcttgac gatctggaac gtgcggccga tgccgagccg ggcggcctcg
      961 tgcggcggcg tgcctgcgag cgagcggccg gcgaaccgga tcacgccctc gtcgggcggc
     1021 aggaagccgg tgatcaggtt gaacagcgtg gtcttgccgg cgccgttggg gccgacgatg
     1081 aacagcacct cgccctcggt gatggtgagc gacacgtcgt tgacggcgcg cagcccgccg
     1141 aaccgcttgc tgacgtgctc gagggtgagc agcgcggtca tgacgcgcgc tgcctccgcc
     1201 gccagcgcgc gatcgtgccg acgatgccct cggggaggaa cagcacgacg agcacgaaca
     1261 ggacgccgta gatcagctgg aaggcctcgt ggaaccagtt cttgaacagc tcctgcgcgg
     1321 tctcgatcaa gatcgcgccg acgatcgggc cggtcagcgt gccggcgccg ccgagcatcg
     1381 cgtcgatcgc cggccgctcg gacaggtcga tcgcgaacgc cgagtcgggg acgacggagg
     1441 cgatctggct catgtacagc gcgccgccga gcgcggtgaa cgcggcgctg atcatgaacg
     1501 cgtggagctt gactcgcgca gcgtcgatgc cgcacgcccg cgccgtgtcc tcgtcctcgc
     1561 gcaccgcctg cagctggtag ccgaaccgcg agcgcacgat cagcgcggtc gcggtgaacg
     1621 ccgcggcggt cagcagcacg gcagacacgt agaaccaccg gatcggcgcg ctggggccga
     1681 acagcgcagg cacctggagc ccgacgtcgc cgccggtgag gttatcgagg ttcttggccg
     1741 acagccgcag cacctcggcg aacgcgatcg tcgcgagcgc gaagtacggc ccgcgcaggc
     1801 ggaacgcgac ccggccgatc acggcggcgg cggcgaccgc ggtgacgatc aagagcacca
     1861 gcgtgagcgg gagcgggatg ccgagcttgt cgtggtacag cggcagcgcg taggcgccga
     1921 tgccgaagaa cgcggcgtgg ccgagcgaca gctggccggc aaagccaccg accaggttcc
     1981 aggccaggcc gagcgcggcg aacagcgcga catggaacag cacgtggacg tggtagtcgc
     2041 cctggaacag cggcaccagc aggcacaggc cgaagccggc ggcggcgagg agggcgcgcc
     2101 ggcgcgtcac acccgctggc tcccgaacag cccggccggc ttgagcgaca gcacgagcag
     2161 gaagatcacg aagtcgacga tcgccgccca ctggttgccc cagtacaggc tggtcaggtt
     2221 ctcgatcaca ccgagcacga ggccggcgat cgcggcgccc tcgatcgatc ccatgccgcc
     2281 gagcacgacc atcacgaacg acttgaggtt gtaaccggcg ccgacctcgg ggtacaggta
     2341 cagcgccggc agcagcagcc caccggcgag gccgaccgcc gcgatcccga gtccgaacgc
     2401 gatcgcctgg gtgcgctgca cgttgatccc gatcagcgac gccgcgtagc ggttctgcgc
     2461 gatcgcgcgc agcgcgcggc cgaaccggct cttcatgacg aacaggaaca tgccgctcgc
     2521 cagcgcggcg ctgatcgcga acgacagcag gtcgccgtgg ttgagcgtca ggtgcgggcc
     2581 gaggcggagc agctgggtgg tcagggcgct gttggccttg cgaaagtccc cgctgaaggc
     2641 gagctgcgcg acaccgatca tcatctgggc gatgcccagc gtgagcagga tggtcatgaa
     2701 ctcggagcgc tcggcgatcc ggcgcagcag gccgtggtag gtcgcggcgc cgagcacgaa
     2761 cagcaccgcc gcggcggcga ggaagccgac gtacggcgtg acgccgagcc ggtcgaacag
     2821 caggtacgtc acgtacatgc cgatcatcat gaacgcgccg tgcgcgaagt tgacgatgcg
     2881 catcacgccg aagatcagcg ccatgcccat gccgatcagc gagtacagcg agccggtcag
     2941 cagcccgtcg aggaccgcct gcagcgcctt ccagaactgc gtcgccatcg cgtaccgtgg
     3001 accgcgcgcg ggctactgcc gctcggacca cttcggcgtg gtcaggatcg gcttggcctc
     3061 ggcctgatcg ggaggccaca cgaccttgta cttcccgccc tggacctgcg tgatcagcac
     3121 cgggtgagcg ttctggccct tggcgtcgaa tgcgatcggg ccgaacggcg tgtcgaggtg
     3181 ctccttgcgg agcgcgtcgg cgatcttctg gtgatcctcc gaccctgcgt tcttgatcgc
     3241 gtcggcggcg acgagcagcg cggtgtaggc ctgcatgccg tgatacgccg ggtgcgtgct
     3301 ggccagcttg aagtacgcct cgtcgaactg cttggagcct tcccacttgg cgctcggcag
     3361 ccactgcgac accgagaacg tgtactccgc atacttgccg gcgcccttgt cctcgctcgg
     3421 gaactcggcg gccgagaagc cggtgccggc ggcggtcagg aacttcgggt tgaggtcgac
     3481 ctgcgcggtc tgcttcatca gcgtgatcgc gtcgagcagg tacgacgcgt agtagacgac
     3541 ctcgggatcc ttgtccttga cccgctggag cagcgccttg taatcggtct ctttcgcctg
     3601 gtaggcctcg ttggccacca ccgtcatccc cgcggccggt gcggcggcct ccatcgcctt
     3661 gccgttggac tgcccgaagt tggtgttctc gtagatgatg gcaaccgtct tgggggcgcc
     3721 gttgttcttg agaaagtcgg ccatcgcggc ggcgtaggcc ttcgcgccgg cgcagatgcg
     3781 gaatacccag ggcgagccgg tctcgaccgt gttgtcggcg gtggcggtcg gcatgatcag
     3841 cgggacgctc ttgggggtca ccgcccggat cacggcgagc gtggtctcgc tcgagtacgc
     3901 acccaggatg atgggcacgt tatcctgatc gatgagcttg gtcgtgccct gggctgcctg
     3961 gtcgggcttg ccctcatcgt cgtagtagat gatctcgatc tgcctgccga gcaccccgcc
     4021 cttggcgttg atctccgagg cggcgatctc gtagccgagc ttgtgcgcct tgccgaacgc
     4081 cgcctgcgcg ccggtcagcg aggtgatcac tccgatgcgg agggtcttgc ccgcctcctg
     4141 cttgccgccg ccaccgccgc ccttgcaggc cgcggcgggg acgccgaaga gggaagccag
     4201 gacgacagcg tgaggaagcc agcgggagcg catcacgtgg ggcatcctac cccttcctgc
     4261 gtcccgtgcg cgggtttcgc gatttcgagt ttcgatcgtc gcgatctccg aggcactgcg
     4321 cacctgtgca ccatcgatcg cgtcggccgg ccgggatccg cgctatcccc cgacgatgat
     4381 ggggaatgca cgtggcgatt cggacgcagc cgcgcggcgc gacgccatcg gtgcaccggt
     4441 gagaatcagc gttgcgccac cgcgcgcacc ggatcgggcg tgaactgcgc ctcctccgtg
     4501 ctcccgtgga gcgcgagcgt cgaggcgccg ccccccgaga tcagctcggc gacctggtcg
     4561 aagtagccgg tgccgacctc gcgctgatgg cgcgtcgcgg tatagccgtt ggcctcggcg
     4621 gcgaactcgg cctgctggag ctcggagtac gccgccatgc cgccggtctt gtacttgcgc
     4681 gccagctcgt acatcccgta gttgagcgcg tggaagccgg cgagggtgac gaactggaac
     4741 ttgtagccca tcgccgcgag ctcgcgctgg aacctggcga tcgtcgcgtc gtcgaggttc
     4801 ttcttccagt tgaacgacgg cgagcagttg tacgcgagca gcttgttcgg gaactggcgg
     4861 tggatgccct cggcgaactg cttggcctgc gcgaggttgg gcgtcgaggt ctcgcaccac
     4921 accaggtcgg cgtagggcgc gtaggccagg ccgcgcgcga tcgcggtctc gatgccgctg
     4981 accatgcggt agaagccctc ggcggtgcgc tcgcccttgg ccacgaacgg gcggtcgcgc
     5041 tcgtcgatgt cgctggtcag gagcttggcg ctgtcggcgt cggtccgggc gaccaccagg
     5101 gtcgggacgc cgctgacgtc ggcggcgagc cgggccgcga ccagcgtgcg gatgaagtgg
     5161 ctggtcggga ccagcacctt gccgcccatg tggccgcact tcttctcgct ggccagctgg
     5221 tcctcgaagt gcacgccggc ggcgcccgcg tcgatcatcc ccttcatcag ctcgaaggcg
     5281 ttgagcggac cgccgaaccc ggcctcggcg tcggcgacga tcggcgccag ccagtggcgc
     5341 tcgtgcttgt tctcggcgtg gtcgatctgg tcggcgcgga gcagcgcggc gttgatccgg
     5401 cgcaccacgc tgggcacgct gtccaccggg tacaggctct ggtcggggta catctgcccg
     5461 gcggtgttgg cgtcggccgc cacctgccag cccgacaggt agatcgcctt gaggccggcg
     5521 cggacctgct ggacggccat gttgccggtc agcgcgccca gcgcggcgac gtagtcctcg
     5581 gcgtggagca gctcccagag ccgccgggcg ccgagccggg cgaccgtatg ctcgatcgcg
     5641 tacgtgccgc gcaggcggtc gacgtcggcc ttggtgtagg gacggacgat gccgtcgaag
     5701 cgcttggcgt gcagcgcgga gtggtcagac aggctggtcg tcatcgcggt ctcctcgttg
     5761 gttcgtcgat ggtccctccc gggatagctg ttgaagatgt gtatggtcaa cgtccgacgg
     5821 ataggttata cgttgatggt gtttaccgtt gtcaatgaca ccctgttgag tagtaaatca
     5881 tgtcaatgac aaaaccgccc ggtggtgcag caatggagcg tgtcacagag caacggctgg
     5941 gggccaaggt ccgcgggctg cggcgccgtg agggcctgac ccaggtccag ctggccacgc
     6001 agctcgggat ctcggccagc tacctcaacc tgatcgaggc caaccgccga cccctgccgg
     6061 ccaacctgtt gatcaagctg gcccagctgt tcggcgtcga cgtggcagcg tttgccagcg
     6121 acgatgacag ccggctggtc gcggatctca ccgaggcgtt cgccgacccc gtgctcgagg
     6181 gctacgacct gacctcggtc gatgtgcgcg agctcgccgc ggccagcccc gaggcggcgc
     6241 gcgcggtgct ggggctgtac cgcgcctacc aggcgacacg tgccaccgcc gacgacctgt
     6301 cgtcccggct gcgcgacggc gaggccctcc ctgcgcccga cggcacacgg gcgccctcgc
     6361 tcatcccgag cgaggaggtc agcgactacc tgcagcgcgc gagcaactac ttccccgagc
     6421 tcgaggccgc caccgaggag ctcgcggtcc gcgcccggct gaccggcgac gacctctacg
     6481 ccggcctgat ccgccacctc gaccagcacc tcggcgtctc ggtgcagatc gggcactggg
     6541 aggcgatgaa gggcgtgctg cgccgctacg accccgagcg caggacgctc acgctgtccg
     6601 agctgctgcc gacccgcagc cggacgttcc agctcgccca ccagatcgcg atcgtctcgc
     6661 tcggcgacgt cctcgaccgc cacgtcgcgg agggccggtt cgcgtcgacc gagacccgcg
     6721 tgctcgccaa ggtcgcgctc gccaactact tcgccggcgc ggtgctgatg ccgtacgccg
     6781 cgttccacca ggcggcgcgc agcgagcgct acgacatcga cgtgctcggc cggcgattcc
     6841 gggtcggctt cgagcaggtc tgccaccggc tgaccacgct gcgccggccc ggcgccgagg
     6901 gcgtgccgtt ccacatgatc cggctcgacg tcgccggcaa catctccaag cggttctccg
     6961 cgtcggggat ccggttcgcg cggttctccg gcgtgtgccc gcggtggaac gtgttcagcg
     7021 cgttcctcac ccccggcatg atccggatcc aggtctcgcg gatgcccgac ggccagacct
     7081 acttctgcat cgcgcgcacc gtgcagaagg acgccggcgg gttccacgcc cagcagccgg
     7141 tgcagtcgat cggcctgggc tgccagatcg agcacgcgcg ccagctggtc tacgccgacg
     7201 gcgtcaacct cgagagccgc gacaccctgg tgctggtcgg cacgacgtgc cggctgtgcg
     7261 agcgcaccga ctgcgagcag cgcgccgtgc cgtcgctcaa ggtgccgctg cagatcgacg
     7321 agaaccagcg cggcatgtcg ctgtacaccg cggtcgaccg gtgaccgcga gtcgcggctg
     7381 cctcggtcgc ggaaccgggc gtcggccgcg gctggcggta tccgcggtcg gtagcggcgg
     7441 tcggtaacgg cagtcggcag tcggtagcgg cggtcggcag cggcggtcgg cagtcggtag
     7501 cggcggtcgg cagtcggtag cggcggcgtg cggtagcggt gggcggcgag cggtcgggca
     7561 tcgaggcggc ggcggctttg cgagcggtgc ccgcagccga aggcgcacca gtggacccag
     7621 ctcgcctcgc ggcgcctcct ccctccgcgg ctgggccgcc ctgc
//
LOCUS       DASVOR010001326        10921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_481353,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001326 DASVOR010000000
VERSION     DASVOR010001326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10921
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481353"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1167
                     /locus_tag="VF516_25315"
     CDS             <1..1167
                     /locus_tag="VF516_25315"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8111084.1"
                     /translation="LGGTGFLGPKTVAAAIARGHQVTIFNRGKREKLLPLEAKVEHLY
                     GNRDPDLPADDERGPDGKLLHPDTGPKGLEQLVGKRWDVVIDNSGYFPRMVGASAQLL
                     AKQAELYIYISSISAYDDAAVPPAGGDETTALATLADPTVETMGKQFENYGGLKALCE
                     RAAAAAFPGRTAIVRPGYIVGPGDPTDRFTYWPVHIARGGEILAPGSPDDPLQWIDVR
                     DLAEWLVALAEHGTAGEFNAIGPPHPARWGDVLQACVTAAHSDARLVWIPATWLEQNG
                     SGGEDAFPIWVAPTGKYAGFHRWSTERARAAGLTYRPVADTVQAILDWYPGEIERRVR
                     VTRELVEQARAKGQPPPQGDPSALRAGPPREREQELLAKWKASGAGATGAAGKG"
     gene            complement(1309..3033)
                     /locus_tag="VF516_25320"
     CDS             complement(1309..3033)
                     /locus_tag="VF516_25320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111085.1"
                     /translation="MSALVVAALGLAGRAHAIPPNAPANLRPGANHHTGDDAFVENQG
                     RPPRPDEEKLRMLEHFRAVHARLAARKATRPELQATRDRLLAHLATYIARGTTPENTH
                     LPWRTPVFIDDQGVICAVGYLIEQSAGRALAERIAAEHRYDYIEDIARDMAEVRAWVE
                     SSGFTLDELGQIQPGYPGPDVLLWHDRQFVDDGDDMPRDDTGIPDGPYQRDGMRGAFV
                     NHRMEGMWELRGPRELDPDADLPRGTRAELASDVVLGHGEFHRGRGHWVSFYPSGARL
                     AVGDYVDDAPSGRWRFYHESGALAAEGRFVSGQRTGPWRFYYDEQRPTPIAIGRFSSD
                     GHVEGRWRHFDANGELLATSRTETPASWRHKGQLFWSVGYLVDVVPTSDGIVHQIHQG
                     AIDGEELRLERIATPDRREQLYAREHRGDAWTASIGGDPDALDVFDRTGAQLVNDGES
                     WRADPCGWSAARRRAARAGDVVTLHGLLDQGSSYQSPSDEPECTRGPLLDPARAARIS
                     ALVTKLTEVRAASPGFLKRLVLDSSRGDDPDLTRVIASTMALDIEWPHVDRRFIQVYQ
                     TLPGYRHI"
     gene            complement(3252..4481)
                     /locus_tag="VF516_25325"
     CDS             complement(3252..4481)
                     /locus_tag="VF516_25325"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8111086.1"
                     /translation="MERQDHSSRGALAMFVVLLLSYVINAMDRQIFSVLAADVRQALS
                     IDLPRIGLASTIFTLGMGLAGLPTGYLLAKMSRRNVTLIGLALFSVATYLTAYSQGLG
                     DLLIYRFLSGLGEAMQLTALLAIGTTYFYRYRAMAASSLNFTFGIGAILGPNIGAAIL
                     HHYDWKAPFMAFGIAGLPALILIVLFVRSWFTEAAPPTGAQLAAAAARPDRDEQNAAT
                     SLTARRPLLLALATVFTGLSIYGYLGLYPTYLREGVKFTPQQAAWCVSFYGLGALLSL
                     LGGWLGDKYNYRKLLFVSLIISAISGGFAFMPLGGSVFLHAVCSFVFGAAISGMGYAN
                     LSAGLIKSIVSAKASQASGLFVASLYIPAAFAGYILGLMKGPLGWTTAGILQLAGSAV
                     VAAILVMASGSKSAAGS"
     gene            complement(4531..5379)
                     /locus_tag="VF516_25330"
     CDS             complement(4531..5379)
                     /locus_tag="VF516_25330"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8111087.1"
                     /translation="MGNRPEFRLPNGARIAVWTIVNVENWQPEHAQPRTVLPPPMGIP
                     LLPDIPNWCWHEYGMRCGFWRFLEVLQKRQLKATFAVNGSACEVYGEACRAAHEAGWE
                     FMGHGFVQKPMHKVEDEAQAIAQTMAAIRGLTGTPPRGWESPGLAETGVTLDLLAEAG
                     IEYVADWVLDDQPVRLKTRSGSIVSVPYTVEINDVVMSAVQLHRSDEIFLRGRDQFDQ
                     LYKEGKSIPRVMAITIHPYLTGVPHRIRYLEALYDHILAHDDVIMATGGEILDYFRNS
                     PAGAIA"
     gene            6176..7870
                     /locus_tag="VF516_25335"
     CDS             6176..7870
                     /locus_tag="VF516_25335"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8111088.1"
                     /translation="MHRDLESMPLLPPSATAAGRSVIAVIGINQYHAWPRLSNAVSDA
                     EGAARLFERLGFVEVTRLIDGAATREALHRLVTGDLIQLTPDDSLVLFFAGHGHTQVR
                     TAGGTSVKTGYVIPVDAADGSQDAGATWLRLDNWLSDIARLPPRHILVIIDACHSGCA
                     LNELYKWRDGTALSTTQLEVLRARRSRRIITSALDDQRAMDSGPYLGHSLFTGCLIEG
                     LSGGLARGEQCVTTGRELGEYLHKRVRNFPGSMQTPDVGAFELDDRGDIVIPILSVEQ
                     ASASVPTQTAPSPQPIPVPPLDAADGRRRTVWFAAGAGVIVAAAIAIVVVATRGTPAP
                     AGTPVAAANESPEAATGPAAVPPPSPAAPSGPPPAAASPLQPAQPADEVKPLEPVSAA
                     AEPSPQRPAHHGSTSSRPAVPASGKRHDAAPAEPARDAGSNPTERTDVADEPARHAES
                     NPTEHTDAAAEPPARVDPKTTAANPDVPPKGEMAAGSGSAAPRSAASKWGRLMDDKTQ
                     GGTDDYLFASCKTAATNGNCDLAKAIAARIAEKNIALYRTRVLTDAAIAACLTAQK"
     gene            7849..8838
                     /locus_tag="VF516_25340"
     CDS             7849..8838
                     /locus_tag="VF516_25340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111089.1"
                     /translation="MSDCAEIRRYVMTRDVMTTRWSGLVALLVALNGPLVSAQPQDRK
                     AAAAALFENGIRDMHAGQTERACSELAESVATWPDSGAKGALAECDTALGRLSDAWEL
                     WRDLATTAPTQDLRDDATQNAAALDRKLVRIVLRLRGQAPPTLVVTINGKPFSTLANA
                     ERRIEPGTLVVDATSPDTAPWTQTLSAQAGTTITVEILLRPSSAPLRRQRSFQVMGLS
                     LLVAGAVGLGVGAASGGMAYVDWRSASDACGGNTGHCKGAGFASAQSDLDHARTAATI
                     STVSSGIGAGVAALGALLYIFNRPRASESETAWRVAPLAGSQTMGLVLSRSMP"
     gene            8835..>10921
                     /locus_tag="VF516_25345"
     CDS             8835..>10921
                     /locus_tag="VF516_25345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111090.1"
                     /translation="MTTALRIGVALCAIHLVACSEILGLEEIVPDGSPARLSFIAPAL
                     SPVAPPPAVVQEPLSVVAVRVEDALGNRVQSPGTEIRLALGANPGGASLLGTLTAKVS
                     SDTALFELVYLDKPGAGYTLVASADGLMPATMMIDVVPAAFTRAPTGVDGESVSGVAA
                     SPAPPGGSSTLFATASGLVFTSTNGGVSWAPPGIGGSSATSVVADPQHPGVAYAIPRG
                     GLQRYLFQKTMDGGMTWHSATIDGANLQQFDEGTIAIAIDPTNSSILYVATYVATYPP
                     DPTTRLHRSADGGMTWTTSSIPVRCSSIVIDRATTSNLYCTNDSDNLLRSTDSGATWR
                     ADTALDARGASLMLATPGALFVNVVGQLYRSTDHGGSWTPLSALNPGALAFAPSMPQR
                     VYLGQGGSLLVSNDVGTSFGPAGASLPFSILQLTVDAQNPDLVYATTGGGVFRSSNAG
                     VSWSPASTGIDATPITAVVPVPGTPGAWLASTPHSVVRTTNDGAMWTQVAQISSAQLA
                     LDPANAANVYACGDLFAASTDGGAHFGAATDSGVFCRYFVAEGNTLFASNGGILRKSI
                     DRGVSWTDVGGIDTSTFIRGFAIGDATGSVVLVGTTAGLRRSTDGGTSFQLVSSNYVL
                     SLFSDPHNPSHVLMGTESCALSYSTDGGSTFKTASSPRLCLSAYGAADGMLYAGGYPY
                     FANGGNLIRSPDGG"
BASE COUNT         1851 a         3696 c         3665 g         1709 t
ORIGIN      
        1 ctcggcggca ccggcttcct cggtcccaag accgtcgccg ccgcgatcgc gcgcggccac
       61 caggtgacga tcttcaaccg cggcaagcgc gagaagctcc tgccgctcga ggccaaggtc
      121 gagcacctct acggcaaccg cgatcccgac ctgccggccg acgacgagcg cggccccgac
      181 ggcaagctgc tgcaccccga taccggcccc aaggggctcg agcagctggt cggcaagcgc
      241 tgggacgtcg tgatcgacaa ctccggatac ttcccgcgca tggtaggtgc ttccgcccag
      301 ctgctcgcca agcaggcgga gctctacatc tacatctcca gcatctcggc ctacgacgac
      361 gccgccgtgc cgccggcggg cggcgacgag accacggcgc tcgccacgct cgccgatccg
      421 acggtcgaga ccatgggcaa gcagttcgag aactacggcg ggctcaaggc cctgtgcgag
      481 cgcgccgcag ccgcggcgtt ccccgggcgc acggcgatcg tccgccccgg ctacatcgtc
      541 ggcccgggcg atccgaccga tcgcttcacc tactggccgg tccacatcgc gcgcggtggc
      601 gagatcctcg cgcccggctc gccggacgat ccgctgcagt ggatcgacgt gcgcgatctc
      661 gccgagtggc tggtcgcgct cgccgagcac ggcaccgcgg gggagttcaa cgcgatcggc
      721 ccgccccacc ccgcgcgctg gggcgacgtc ctgcaggcgt gcgtcaccgc ggcccacagc
      781 gatgccaggc tcgtgtggat cccggcgacc tggctcgagc agaacggctc gggcggcgag
      841 gacgcgttcc cgatctgggt cgcgccgacc ggcaagtacg ccggcttcca ccgctggagc
      901 accgagcgcg cgagggccgc gggcctgacg taccggccgg tcgcggacac ggtccaggcg
      961 atcctcgact ggtacccggg cgagatcgag cgccgggtcc gcgtgacccg cgagctcgtc
     1021 gagcaggcca gggccaaggg ccagccgccg ccgcaaggcg atccgagcgc cctgcgcgcc
     1081 ggcccgccgc gcgagcgcga gcaggagctg ctcgcgaagt ggaaggccag cggcgccggc
     1141 gcgaccggag ctgccggcaa gggctgagcg gaccgttcac acgccggctg cgaatcattg
     1201 ctcctcacat gcggattgcc aatcagatag gaaatgggat caggaagcga tcgggaaggg
     1261 gagtcctggg aggaccgtat tcctgatgat gcctcagctc gcgcagcgtc agatgtggcg
     1321 atagcccggc agggtctggt agacctggat gaaccggcgg tcgacgtgcg gccactcgat
     1381 gtcgagcgcc atcgtggacg cgatcacccg cgtcaggtcg gggtcgtcgc cacgcgagct
     1441 gtccagcacc aggcgcttga gaaagcccgg gctggcagcg cggacctcgg tgagcttggt
     1501 cacgagcgcc gagatccggg ccgcgcgcgc ggggtcgagc agagggccgc gcgtgcactc
     1561 cggctcgtcg gacggcgact gatagctgct gccctggtcg agcaggccgt gcagggtcac
     1621 cacgtcgccg gcacgcgccg cgcgccgccg cgctgcgctc cacccgcatg gatcggcgcg
     1681 ccaggactcg ccgtcgttga ccagttgcgc gccggtgcga tcgaacacgt cgagcgcgtc
     1741 gggatcgccg ccgatcgacg cggtccaggc gtcgccgcgg tgctcgcgtg cgtagagttg
     1801 ctcgcgccgg tccggcgtcg cgatccgctc gagccggagc tcctcgccgt cgatcgcgcc
     1861 ctggtggatc tggtggacga tgccgtccga ggtcggcacg acgtcgacca ggtagccgac
     1921 cgaccagaac agctggccct tgtggcgcca gctcgcgggg gtctccgtcc gagaggtcgc
     1981 gagcagctcg ccattggcgt cgaagtggcg ccagcgaccc tcgacgtggc cgtcgctgct
     2041 gaaccggccg atcgcgatcg gcgtcggcct ctgctcgtcg taatagaacc gccacggccc
     2101 ggtgcgctgg ccgctcacga accggccctc ggccgccagc gcgccgctct cgtggtagaa
     2161 ccgccaccgc ccgctgggcg cgtcgtcgac gtagtcgccc acggcgagcc tggcgcccga
     2221 cgggtagaag ctcacccagt gcccgcgccc gcggtggaac tcgccatggc cgagcacgac
     2281 gtccgaggcg agctccgcgc gtgtgccacg cggcagatcg gcgtccggat ccagctcgcg
     2341 cggcccgcgc agctcccaca tgccttccat gcggtggttg acaaacgcac ctcgcattcc
     2401 gtcgcgctgg tacgggccgt cgggaatgcc ggtgtcgtcg cgcggcatgt catcgccgtc
     2461 gtcgacgaac tggcgatcat gccagagcag cacgtcgggg cccggatacc cgggctggat
     2521 ctggccaagc tcgtcgagcg tgaaccccga ggactcgacc cacgcgcgga cctcggccat
     2581 gtccctggcg atgtcctcga tgtagtcgta gcgatgctcg gccgcgatcc gctcggcgag
     2641 cgcgcggccc gccgactgct cgatcagata gccgaccgcg cagatcacgc cttgatcgtc
     2701 gatgaatacc ggcgttcgcc agggcaggtg cgtgttctcc ggcgtggtcc cgcgcgcgat
     2761 gtacgtggcg aggtgggcga gcaggcgatc tcgggtcgcc tgcagctccg gccgggtcgc
     2821 cttgcgcgcc gccagccgcg cgtgtactgc tcggaagtgt tcgagcatcc ttagcttttc
     2881 ttcatccgga cggggcggcc ggccctggtt ctcgacgaat gcatcgtctc cggtgtgatg
     2941 gttcgcgcca ggtcggaggt tggcgggcgc gttgggcggg atcgcgtgcg cccgaccggc
     3001 caggccgagc gcggcaacga cgagcgcgga catcgatcgg gtgcgcatct ccgtctgccc
     3061 cagcacgcgc gataccagcg gcggggcacg ccttcagccg acttggccgc gggtgtgggc
     3121 gagcggacac ggtcggcgtg tccaccaggc cgctactcat ggcacgagct tgacccgcca
     3181 ggtgcctgag atctcctgga taagactgca ccgcctgggt cgcgccggcg gcgtcgaagt
     3241 cgaggtgcgg ctcacgatcc cgcagcagat ttcgaaccag aggccatcac caggatggcg
     3301 gccaccacgg cgctgcccgc cagctggagg atacctgccg tcgtccaccc cagaggtcct
     3361 ttcatcagcc caaggatata tcccgcaaac gcggcgggaa tatacaggct ggcgacgaac
     3421 agcccggaag cctgcgacgc tttcgcgctg acgatggatt tgatcagccc ggcggaaagg
     3481 ttggcgtacc ccatgccgct gatggccgcg ccgaacacga aggaacatac cgcgtgcagg
     3541 aacacggagc cgcccagagg catgaacgcg aaaccaccgg atatggccga gatgatcagg
     3601 gagacgaaca gcagcttgcg atagttgtat ttgtcgccca gccagccccc gagcagcgaa
     3661 agcagcgcgc ccagaccata gaagctcacg caccacgccg cctgctgggg agtgaacttc
     3721 acaccctcgc gcagatacgt tggatagagg ccgaggtagc cgtaaatcga cagcccggtg
     3781 aagaccgtgg ccagagcgag cagaagcggc cttcgggcgg tcagcgaggt ggcggcgttc
     3841 tgctcgtcgc gatcgggtcg ggctgccgct gccgcaagtt gcgcgccggt gggaggcgcg
     3901 gcttcggtga accaggatcg cacgaacagc acgatcagaa tcagggcggg cagaccggcg
     3961 atgccgaacg ccatgaatgg agccttccaa tcgtagtggt gcagaatggc cgccccgata
     4021 ttggggccga gaatggcacc gatgccgaag gtgaaattga gggagctcgc cgccatggcc
     4081 cgataccggt agaaataggt ggtgccgatg gccagcaggg ccgtcaattg catggcttca
     4141 ccgagaccgg acaagaatcg atatatcaat agatcgccga gaccctggga ataggccgtc
     4201 agataggttg ccacagaaaa cagtgccaac ccgatgaggg tcacgtttct cctcgacatt
     4261 ttcgccagca ggtaccccgt cggcagtccg gccagtccca tgcccagcgt gaagatggtc
     4321 gaagcaagac cgatgcgggg cagatctatc gacaacgcct gtcgcacgtc agccgccagg
     4381 accgagaaga tctggcgatc catggcgttg atgacgtagg agagcaagag cacgacgaac
     4441 atcgccaggg cgccgcggga actatgatcc tgtcgttcca tgattgagct ccttatcgaa
     4501 ggtcgtactt tctgtgcctg ccctgatcga tcaggcgatc gcgcctgccg gggaattcct
     4561 gaaataatcc aggatttcgc ccccggttgc catgatcacg tcgtcgtggg ccaggatatg
     4621 gtcgtacaga gcctcgagat atcggatgcg gtgcggcacg ccggtcaaat aggggtggat
     4681 ggtgatcgcc atgacccggg gaatcgattt cccctccttg tataattgat cgaactgatc
     4741 gcggccccgc aggaatattt cgtccgaccg gtgtagttga acggcgctca tgacgacatc
     4801 gttgatctcg accgtatagg gcacggaaac gatactgccg gagcgggtct tcaggcgcac
     4861 cggctggtca tccagcaccc agtcggccac gtattcgatc cccgcctccg cgagaaggtc
     4921 gagagtcaca cccgtctccg ccaggccggg actctcccac ccgcgcgggg gggtgcccgt
     4981 caggccgcgg atggccgcca tggtctgcgc aatggcctgc gcctcgtctt cgaccttgtg
     5041 catgggcttc tgaacaaacc cgtgtcccat gaattcccag ccggcttcgt gcgccgcccg
     5101 gcatgcctcg ccgtagacct cacaggcact tccgttgacg gcaaaggtcg ccttgagctg
     5161 gcgcttttgc aggacctcca gaaagcgcca gaacccgcac cgcatgccat actcgtgcca
     5221 gcaccagttg ggaatgtcgg gaagcagcgg aatccccatc ggcggaggca gcacggtcct
     5281 cggttgggca tgttccggct gccagttttc cacgttgacg atggtccata cggcaatccg
     5341 cgccccgttc ggcaaacgga actcgggccg gttccccatg ggctgatagg acaaccgatc
     5401 tctggaggtc atgggatgtc gcgcttgttc catactcttc tcctgaagga cgtcgatgga
     5461 atgaggtaat acttgccgcc ggccatgggg aaacattcag attctaagct gattttgaaa
     5521 caagtaaaag cgtgtactga tcgtccggga ccgcccgagc atcgaagaca cgtccaggac
     5581 tggactcgtc cacctccgcc ggccgacatc gaggagcact gactgtcatc gagcccacga
     5641 taagcgcttc gctgcgggtc gaagtccttc acgatcgcca ggaagccgcg ccggctcggc
     5701 catgtcgacg cggccgcggt acccgtggtc gccgtgaccg cgctgcagat gctggtcgat
     5761 cgtgctgcag gcgcgctagg gcgggcatcc ggccactccg agcgatcggt cgtcgatgcc
     5821 cgcgagaaat cctgcccggt aggcgacggc tcgcggcaaa ggacgacatg gcttcactcc
     5881 ggatgggtgc acctggaacc cgcttgaccg gcaaggccgc cagcgatggg ttcaaggcgc
     5941 tggagcagcg cggccagtga accgcaggtt aggcggcagc ggttgcagag cggtcgaccg
     6001 tacgatggtt ttcggtcgta gttaccggcg agtgcgtcgt ccggttcgcg tccgaccctt
     6061 cgcaccgcgg ctcagcccac cccccggtgg gccggcttga atggccggct cgcggcctcg
     6121 cgtcgtattg tggctgctcg tgtcgcctaa tgtcgcgcct gacgacaggt gccccatgca
     6181 tcgcgacctt gaatcaatgc cactccttcc gccgagcgcc acagccgccg ggcgctccgt
     6241 catcgcggtg attggaatca accagtacca tgcctggccg cgtctctcca atgccgtgag
     6301 cgacgctgaa ggtgcggcaa ggttgttcga acgccttggc ttcgtcgagg tgacccggct
     6361 gatcgacggg gccgccacac gggaagccct gcaccgcctc gtcaccggtg atctgattca
     6421 gctgacaccg gacgatagcc tggtgttgtt cttcgccggg cacgggcaca cgcaggtcag
     6481 gacagccggc ggcacgtcgg tgaagaccgg ttatgtaatc cccgtcgatg ctgccgatgg
     6541 cagccaagat gcgggggcga catggttgcg actggacaac tggctcagtg acatcgcgcg
     6601 acttccaccg cgccatatcc tcgtgattat tgacgcgtgc catagcggat gtgctctcaa
     6661 cgagctctac aagtggcgtg atggcacggc attgtcgaca acgcagctcg aggtgcttcg
     6721 cgccaggcgc agccgccgca tcatcacatc cgctctcgat gatcaacgag caatggacag
     6781 tggtccatat ctggggcatt cgttatttac cggctgtttg atcgaaggac tgtctggtgg
     6841 gctcgcccgg ggtgagcagt gcgtgacgac cggccgagag cttggcgaat acctccataa
     6901 gcgggtgagg aactttccgg gctccatgca gacaccggat gtcggcgcct tcgaactgga
     6961 cgaccgcggt gatatcgtca tcccgatcct gagcgtcgag caggcatccg cgtcggtgcc
     7021 gacgcagaca gcgccatcgc cccagccgat tccagtgccc ccgctggacg ctgccgatgg
     7081 acggcggcgc actgtctggt tcgcagccgg tgcaggcgtg atcgtggctg cagcgatcgc
     7141 gatcgtcgtg gtcgcgacgc gcggtacgcc ggcgccggcc gggactcccg ttgctgcggc
     7201 caatgagtcg ccggaagccg cgacgggccc cgcggcagtc cctcccccga gccccgccgc
     7261 gccttccggg ccgccgccag cagcagcgag tcccctccag ccggcccagc ccgcggacga
     7321 ggtcaagcca ctcgagccgg tgagcgcggc ggccgagccg tcaccgcaac ggccggcgca
     7381 tcacggctcc acatcgtcca ggccagcggt cccggccagc gggaagcgcc atgacgccgc
     7441 gccggccgag ccggcgcggg acgcgggatc gaatccgacc gagcgcaccg acgtggccga
     7501 cgagccggcc cggcacgcgg aatcgaaccc gaccgagcac accgacgcag ccgccgagcc
     7561 gccggcacgc gtcgatccga agacaaccgc ggccaatccc gatgtgcctc caaagggcga
     7621 gatggccgca ggcagcggca gcgccgcgcc caggtccgcg gcgagcaagt ggggacggct
     7681 gatggacgac aagacccagg gcggcaccga tgactatcta ttcgcgagct gcaagacagc
     7741 cgcgacgaat ggcaactgcg acctggccaa ggcgatcgcg gcacggatcg ccgagaagaa
     7801 catcgcgctc taccgaacga gggttctgac ggacgccgcg atcgcggcat gtctgactgc
     7861 gcagaaataa ggaggtacgt gatgacaagg gacgtgatga cgacaaggtg gtcgggcctg
     7921 gtggcgctgc ttgtggcgct caacggtccg ctcgtgagcg cgcagcctca ggaccgcaag
     7981 gcggccgcag cggcgctgtt cgagaacggc atccgcgaca tgcacgccgg ccagaccgag
     8041 cgcgcttgca gcgagctcgc cgagagcgtc gcgacctggc ccgacagcgg cgccaagggt
     8101 gcactggcgg agtgcgatac cgcgctcggg cggctgagcg acgcctggga actgtggcga
     8161 gatctcgcga cgacggcgcc cacgcaggat ctccgcgacg acgccaccca gaacgccgcg
     8221 gcgctcgacc gcaagctggt gcgcatcgtg ctgcggctcc gcggccaggc gcctcccacg
     8281 ctcgtggtga cgatcaacgg caaaccgttc agcacgctcg cgaatgccga gcggaggatc
     8341 gagccgggga cgctggtcgt cgatgccacg tcgccggaca cagcgccgtg gacgcagacg
     8401 ctctccgcgc aagcgggtac gacgatcacc gtcgagatct tgctgcgacc atcctcggcg
     8461 cccctgcgcc gacaacggag cttccaggtc atgggcctgt cgctgctcgt cgcgggggcc
     8521 gtagggctcg gcgtcggggc agcgtccggc ggcatggcct acgtcgactg gcgaagcgct
     8581 tccgacgcct gcggcggcaa caccggtcac tgcaaggggg cgggtttcgc gagcgcgcag
     8641 agcgacctcg atcatgcgcg caccgcagcc acgatctcca cggtgtcgag cggcatcggc
     8701 gcaggcgtcg ctgcgctcgg ggcgctgctc tacatcttca acagacccag ggcctccgag
     8761 tccgagaccg cctggcgggt cgctccgctg gcaggctcgc agacgatggg cctcgtgctg
     8821 tcacggagca tgccatgacc acagccctgc ggatcggcgt tgccctgtgc gcgatccatc
     8881 tggtcgcgtg cagcgagatc ctcgggctcg aggagatcgt cccggatgga tctccagccc
     8941 ggctgtcgtt catcgcgcca gcgctgtcgc ccgtcgcacc gccgccggcc gtcgtccagg
     9001 aaccgctgag cgtcgtcgca gtgcgggtcg aggacgcgct cggcaaccgt gtccagagcc
     9061 ccggtacaga gatccggctg gcgctgggcg ccaatcccgg cggagccagt ctgctgggca
     9121 cgctcaccgc caaggtcagt tcggacaccg ccctgttcga actcgtgtac ctcgacaagc
     9181 cgggcgcggg ctacacgctg gtcgcgtccg ccgacgggct catgccggcg acgatgatga
     9241 tcgatgtggt gccagcggcg ttcacccgcg cccccaccgg tgtcgatggc gaaagcgtgt
     9301 cgggcgtcgc cgcgtcgcct gctcccccag gcggatcgag cacgctgttc gccaccgcca
     9361 gcggcctcgt gttcacgtcg accaatggtg gcgtgagctg ggcacctccc ggcatcggag
     9421 ggagcagcgc gaccagcgtc gtggcggatc cgcagcatcc aggcgtcgcc tacgcgatac
     9481 cgcgtggggg gctccagcgg tatctgttcc agaagacgat ggacggaggc atgacctggc
     9541 acagcgccac gatcgacggc gccaacctgc agcagttcga cgaggggacc attgcaatcg
     9601 ccatcgatcc gacgaattct tcgatcctct acgtggcgac ctacgtggcg acctacccgc
     9661 ccgacccgac caccaggctt caccgcagtg ccgacggcgg catgacctgg accaccagca
     9721 gcatacccgt cagatgcagt tccatcgtca tcgatcgcgc gacgacgtcc aacctgtact
     9781 gcacgaatga cagcgacaac ctgctccggt ctaccgactc aggggcgacg tggcgcgccg
     9841 atactgcgct cgacgcgcgc ggggcgtccc tgatgttggc gacgcctggt gcgttgttcg
     9901 tgaacgtggt cggccagctc taccggtcga ccgaccacgg cggctcatgg actccgctct
     9961 ccgcgttgaa ccctggtgcg ctggcattcg cgccgtcgat gccgcagcgg gtctatctgg
    10021 gccagggtgg ctcccttctg gtgtccaatg atgtcggcac cagtttcggc cccgccggcg
    10081 cgagcttgcc gttctccata ctccagctca cggtcgacgc gcagaatccc gacctcgtct
    10141 atgcgaccac aggcggcgga gtgttcagat ccagcaatgc cggagtgagc tggtcgccgg
    10201 cctcgaccgg catcgatgca accccgatca ccgcggtcgt gccggtcccg ggcacgccgg
    10261 gagcgtggct ggcctcgacg ccgcattcgg tcgtccggac caccaacgat ggggcgatgt
    10321 ggacgcaggt ggcgcagatc tcctcggcac agctcgcgct cgatccggcg aacgccgcaa
    10381 acgtgtacgc atgcggagac ctgttcgccg cgtcgacgga cggcggcgcg cacttcggcg
    10441 cagccacgga ctctggtgtg ttctgccgtt acttcgttgc tgaggggaac acgttgtttg
    10501 catcgaacgg gggcatcctg cgcaagtcga tcgaccgggg tgtgagctgg acggacgtcg
    10561 gtggcatcga cacatcgacg ttcatccgcg gcttcgccat cggtgacgcc actggttcag
    10621 tggtcctcgt cggcaccact gccggactcc gccgatcgac cgacggtggc acgagcttcc
    10681 agctggtgag cagcaactac gtgctcagtc tgttctcgga tccgcacaac ccgtcgcatg
    10741 tcctcatggg gacggaatcc tgcgcgctta gctactccac cgacggcggg tccaccttca
    10801 agacggcgag cagcccgcgc ctctgtcttt cggcctacgg cgcggccgac ggaatgctct
    10861 atgcgggcgg ctacccctac ttcgccaacg gaggaaatct gatcagatcg cccgacggag
    10921 g
//
LOCUS       DASVOR010001327         1643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_422047,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001327 DASVOR010000000
VERSION     DASVOR010001327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1643
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422047"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1643
                     /gene="glgB"
                     /locus_tag="VF516_25350"
     CDS             <1..>1643
                     /gene="glgB"
                     /locus_tag="VF516_25350"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX8111091.1"
                     /translation="ELDAHLMREGTHPRLHDKLGAHPTPDGTRFAVWAPSALAVSVIG
                     DFNDWTPGAHPLHELPGTGGVWSGEVSGVRRGALYKYHVRSRLRGYEVAKADPFAIRN
                     ECPPHTASIVWTADHAWGDAAWMAGRAARSRPDAPVAIYEVHLGSWMRVPEDGNRWLT
                     YREIAPRLVEHVTRVGFTHVELMPLTEHPFYGSWGYETTGYFAATARYGTPEDLMALV
                     DALHQAGIAVLLDWVPAHFPSDEHGLIYFDGTPLFEHPDRRLGFHPEWNTSVFDFGRP
                     EVRSFLLSSALYWLEQFHIDGLRVDGVASMLYRDYARKPGEWIPNARGGREYFEAVEL
                     LQQLNTAIAREQPAAITIAEESTAWPHVTGPVDAGGLGFSYKWDMGWMHDTLAYLAHD
                     PVHRKYHHGQLTFRGLYAFSERFVLPLSHDEVVYGKRSLLGRMPGDRWQQFATLRLLY
                     AYMYSQPGKKLLFMGSEFGQVNEWNHDASLDWHLLDDPMHRQVQLLIGELNRLYREEA
                     ALHELDCEPAGFQWLNADDAERSVLIYERIARDGRRVIAA"
BASE COUNT          241 a          611 c          551 g          240 t
ORIGIN      
        1 gagctcgatg cccacctgat gcgcgagggc acccatcccc gcctgcacga caagctcggc
       61 gcgcatccga cgcccgacgg cacgcggttc gccgtctggg cccccagcgc cctcgcggtc
      121 agcgtgatcg gcgatttcaa cgactggacc ccgggcgcgc acccgctgca cgagctgccc
      181 ggcaccggcg gcgtgtggag cggcgaggtg tccggcgttc gccgcggcgc gctgtacaag
      241 taccacgtgc gatcccgcct gcgcggctac gaggtcgcca aggccgaccc gttcgcgatc
      301 cggaacgagt gcccgccgca caccgcgtcg atcgtgtgga ccgccgacca cgcgtggggc
      361 gacgcggcgt ggatggcggg tcgcgcggcg cggtcccggc ccgacgcgcc ggtcgcgatc
      421 tacgaggtcc acctcggatc gtggatgcgc gtccccgagg acggcaaccg ctggctgacc
      481 taccgcgaga tcgcgccgcg gctggtcgag cacgtcaccc gcgtcgggtt cacccacgtc
      541 gagctgatgc cgctgaccga gcacccgttc tacggctcgt ggggctacga gaccaccggc
      601 tacttcgccg cgaccgcgcg ctacggcacc cccgaggatc tgatggcgct ggtcgacgcg
      661 ctccaccagg ccggcatcgc cgtgctgctc gactgggtgc cggcgcactt cccgagcgac
      721 gagcacggcc tgatctactt cgacggcacg ccgctgttcg agcaccccga tcgccggctc
      781 ggctttcacc ccgagtggaa cacctcggtg ttcgacttcg gccggcccga ggtccggagc
      841 ttcctgttgt cgagcgcgct gtactggctc gagcagttcc acatcgacgg cctgcgggtc
      901 gacggcgtcg cctcgatgct gtatcgcgac tacgcgcgca agccgggcga gtggatcccc
      961 aacgcgcgcg gcggccgcga gtacttcgag gcggtcgagc tgctgcagca gctcaacacc
     1021 gcgatcgccc gcgagcagcc cgccgcgatc acgatcgccg aggagtcgac cgcgtggccg
     1081 cacgtcaccg gcccggtcga cgccggtggc ctcggcttct cgtacaagtg ggacatgggc
     1141 tggatgcacg acacgctcgc ctacctcgcc cacgacccgg tgcaccgcaa gtaccaccac
     1201 ggtcagctca cgttccgcgg cctctacgcg ttcagcgagc ggttcgtcct gccgctgtcg
     1261 cacgacgagg tcgtctacgg caagcgctcg ctgctcggcc ggatgccggg cgaccgctgg
     1321 cagcagttcg ccacgctccg gctgctctac gcgtacatgt acagccagcc cggcaagaag
     1381 ctcctgttca tgggcagcga gttcggccag gtcaacgagt ggaaccacga cgcgagcctc
     1441 gactggcacc tgctcgacga tccgatgcac cgccaggtcc agctgctgat cggcgagctc
     1501 aaccggctgt accgcgaaga ggccgcgctc cacgagctcg attgcgagcc ggccgggttc
     1561 cagtggctca atgccgacga cgccgagcgc tcggtgctga tctacgagcg gatcgcccgg
     1621 gacggccgcc gcgtgatcgc ggc
//
LOCUS       DASVOR010001328         6185 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393027,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001328 DASVOR010000000
VERSION     DASVOR010001328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6185
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393027"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VF516_25355"
     CDS             complement(<1..1077)
                     /locus_tag="VF516_25355"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8111092.1"
                     /translation="MQAPEQSGTTWADPVRASASLEQLTGAPMQVQDFLSLAIRVTSE
                     LAALHARGLIHHDVRPSSIVFERSETGGGDPDGSAGGAGGRAPRGIDAATGVVALVGP
                     PTADRKTAALSEGSLPYISPEQTGQMNRPIDCRSDLYSLGVVFYQLLAGRLPFEAEDA
                     MGWVHCHVARQPRPLDQVRPSVPRPVVDIVGKLLAKLPDHRYQSALGLHHDLERCLRD
                     GKQHGALLSFPLGERDVSEEFRIPHKLYGRDAESAVLREAFERAAASGTAELVLLSGS
                     AGIGKSSLVRELLRAVVQRRGRLIAGKFEQYERNIPYFTITQALRELALDILAEDELR
                     IARWRQQLAQALGPHGKLVVDLVPQ"
     gene            complement(1064..1480)
                     /locus_tag="VF516_25360"
     CDS             complement(1064..1480)
                     /locus_tag="VF516_25360"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8111093.1"
                     /translation="MEPQRSAEGDQKELGVSGFVLIVDDDPDLLDVTSFVIQNEGMSV
                     ETARNGEEALALIGTGRPPALVLLDLMMPVMNGWEFLAAIANDPLLKPIPVVVLTAAE
                     HAEVPGALEVLSKPMDLKELLRVVERYVRGDKGAGA"
     gene            2374..2586
                     /locus_tag="VF516_25365"
     CDS             2374..2586
                     /locus_tag="VF516_25365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111094.1"
                     /translation="MTEISGFTLHPIEIGCSRRGEPRAHPVAEVDRGGWRAPALGVGD
                     VAADRDLAGLSGVAPPQIGPSAGATG"
     gene            2921..5995
                     /locus_tag="VF516_25370"
     CDS             2921..5995
                     /locus_tag="VF516_25370"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HEX8111095.1"
                     /translation="MFAAFVVAAIAVTGCEGPTGPEGPPGQDGSNGSNGASCTLTDNG
                     NGTSTITCPGGTSVTVPTGAKGTSCTVVANADGTRTITCNDGTSVVVTNAVVDYQVMT
                     SDELKASAMVASIKSVTIPTTGPAAGHPLVTIKVTERHGLGVKGLVPVQPDPLHPNPL
                     LPVSWRYALLKLDTGVNGSVDETWVSYMANNSTSTASAETAAAAGFTDNGDGTYTYKL
                     AKDVTGGISAAGTTYEPDKPHRLVILMAGPGNPFSPINLVKDFVPTTGADITSSHEKF
                     DGAACLECHTSFRAIADGTGAFGTGEFHSGGRYDTRTCVACHNDQRRFAKPIVDDAAI
                     GADGTWTGNLAVVNGEATINFPVFIHKIHMGDKLALTGGTYSGIPQPYETTYPQDIRN
                     CTKCHRNPAPNNPAPLAANWKVPSRRACGACHDDKSFVNPPPAGRTLHSGGAMANDNN
                     CLLCHGPGGIGHAPDEIHAPVSPPNPNNIYLNPTSGNSNTNAAYVAAAGFVPPGAKQL
                     TYEVSAVTLDGSRHPQIVFRVLKGDPNANPPVAPAPVTFQDHTAATEMIPGFVGSPSA
                     YFVYAVPQDNITAPADFNVSVSGYLRSIWNGTATGNGAGTMTGPDANGFYTVTLTSDA
                     IKVPTSAVMLTGGIGYTYSLGSATPPSGPPFSNNTQPFTEIDMPSKYPYTPNASGFAG
                     KGGLIVPPPDVSKVADKPFTARRAIVDNSKCDTCHVSLGVGPDFHAGQRNDSATCAWC
                     HRPNQTSSGWAANAKDFIHSIHAAEKRTVPFNWHAESPTEGFFNVTYPAILNRCEMCH
                     LPGTFDFSLTSTTSALPNMLPSTVGQGRYDVKPTSNPSGFFAIAPPQYVTRNNSAFDY
                     GFGFNTSNVNATLPDGISGTQGATSCTPEQPCLCTAANPCSATISGTYAVNNVQVSFT
                     QKISGVTNPCNASTPCTCTTAQPCTGVVATCTPTTPCQAQPTTLVNSPIAAACVACHD
                     SPSAVDHMQTNGASIWEPRSIALNKPQKEECLICHGPNRIAGIALVHTDKTP"
BASE COUNT         1062 a         2248 c         2025 g          850 t
ORIGIN      
        1 ctgcgggacc agatccacga ccagcttgcc gtgcggcccg agggcctgcg cgagctgctg
       61 ccgccaccgc gcgatccgga gctcgtcctc ggccaggatg tcgagcgcga gctcgcggag
      121 cgcctgggtg atcgtgaagt aaggaatgtt gcgctcgtac tgctcgaact tgcccgcgat
      181 cagccggccg cgccgctgga cgaccgcgcg gagcagctcg cgcaccagcg atgacttgcc
      241 gatgcccgcg gaacccgaca gcaggaccag ctccgcggtt ccggacgcgg cggcgcgctc
      301 gaacgcctcg cgcagcaccg cggactcggc gtcacggccg tacagcttgt gcgggatccg
      361 gaactcctcg gagacgtcgc gctcgccgag cggaaacgac agcagcgcgc cgtgctgctt
      421 cccgtcgcgc aggcagcgct ccagatcgtg gtggaggccg agtgcgctct ggtagcgatg
      481 gtcgggcagc ttggcgagca gcttgccgac gatgtccacc acggggcgcg gcaccgacgg
      541 tcgcacctga tcgagcggcc ggggctgacg cgcgacgtga cagtgcaccc agcccatcgc
      601 gtcctcggcc tcgaacggca accggccggc gagcagctgg tagaacacga cgccgagcga
      661 gtacaggtcc gagcggcaat cgatcggccg gttcatctgt ccggtctgct cgggcgagat
      721 gtacggcagc gagccctcgc tcagcgcggc ggtcttccgg tccgcggtcg gcgggccgac
      781 cagcgcgacc accccggtcg cggcgtcgat cccacggggg gctctgcccc ccgccccgcc
      841 ggcagaaccg tcggggtccc caccccccgt ctcgcttcgc tcgaacacga tgctggacgg
      901 ccggacgtcg tggtggatca ggccgcgcgc gtgcagcgcg gcgagctcgc tggtcacacg
      961 gatcgcgagc gacaggaagt cctggacctg catcggtgca ccggtgagct gctcgagcga
     1021 ggcgctcgcg cggacgggat ctgcccacgt cgtgccggac tgttcaggcg cctgcaccct
     1081 tgtctccgcg gacgtagcgc tcgacgaccc ggagcagctc cttgaggtcc atcggcttgc
     1141 tcaggacctc cagtgcgccg gggacctcgg catgctcggc ggcggtcagc accacgaccg
     1201 ggatcggctt gagcaacgga tcgttcgcga tcgcggccag gaactcccag ccgttcatca
     1261 cgggcatcat cagatcgagg agcacgaggg ccggcggtct gccggtgccg atcagcgcga
     1321 gcgcttcctc gccgttcctc gcggtctcga cggacatccc ctcgttctgg atcacgaagc
     1381 tcgtcacgtc gagcagatcc ggatcgtcgt cgacgatgag gacgaacccg gatacgccga
     1441 gctccttctg atcgccttcc gctgagcgct gcggctccac ccaggaagcg accttgggag
     1501 ccgccaaccg gtgcggcgcg atcccagcgt gttcggcggt cgccaccgtc gtcgacgatc
     1561 gaggtccgtc gcaaggctcc gcctgcgcat cgagtgcccc gcgtgattcg agcccccacg
     1621 ccagatgccg ctggaccgtc gccgtggaac gcgaaggcgt cgtcatgatg ctcgggaacg
     1681 tcatcttcgg atcacccatc ttgcgatcac cccctcttca gatcaccccc tcctcaggtc
     1741 accccatctc agaattccat catgctcgtg tgcggcgaga aaatgccacg atgaaagcgg
     1801 acatgcatcg tgaggcaagc ttacgtacgc tcccggctct cggatacgcg actcacggga
     1861 agctcctcgg cgataaccgc gtttcatgaa acctggcgcg cgtcgcttgc cacaggtcat
     1921 gccgaacggg cgactccacg catcgcggcg agcgatggtc ggttcacaag ctcgtacgcc
     1981 ggcgttccgt ggtactggac gtgcgagacg agcgtcggct cggtggctcg ccggacgctt
     2041 cgcaggagga cgtgcgggtg acccaaggta gtacaatggg gctgctcgac gcgatcctgc
     2101 gccgacgctc tgccatagcg tcgtagcttc cgctccgtcg gcgtcaagat cgcgtcaagc
     2161 gccgcccacc cggcgcgaag gtgcgcatgc cccgggtgct gggcggaagc acggacggag
     2221 gcggggcgcg aatcgggtgg gcggcgattg acatcatcac cgtgaggcat aggctcgaat
     2281 gctcgacgac aggaacggga aagggacgat acttgaagtg atcccggagt tcgattcgca
     2341 gcatcgccat tctcgtgagc acgaatcgtg accatgactg agatctccgg gttcactctg
     2401 cacccgatcg agatcggttg ttcacgacgc ggtgagccgc gcgcgcatcc ggtcgccgag
     2461 gtcgaccgcg gtggttggcg cgcgccggcg ctgggcgtcg gcgacgtcgc ggcggaccgc
     2521 gatctcgcgg ggctgtctgg ggtagcgccg ccccagatcg gcccatcggc tggagcgacc
     2581 gggtgagacc cgcggaatgt cctctccgcg aggtccggtg gctgcgcgcg atcggccggg
     2641 cctgcgaggg acctgcattt cctgcgcgga gcgacgaatc gcctgcagag cctgcggggg
     2701 tgcggcgagg tgcgccctgc ccgagcgaac cgcgcagcgg cgaaatgcct gcgaatctcg
     2761 agcgttgcgt cggtcggcga gatctgcgcc cggcggcgcg agaacggcgc ggcggctgca
     2821 aaggcgctga gcagcgacgc acacttcgcg cgcaggcgag ggatccatgt cagatgcaag
     2881 ctccggagaa ggaagtcatc tcttcacgac gaggcgcggc atgttcgccg ccttcgtcgt
     2941 cgctgcgatc gccgtgaccg gctgtgaggg tccgaccggg cccgaaggac cacccggcca
     3001 ggacggcagc aacgggagca acggggcgtc ctgcaccctc accgacaacg gcaacgggac
     3061 ctccaccatc acctgccccg gcgggacgtc ggtgacggtg ccgaccggcg ccaagggcac
     3121 gagctgcacg gtggtcgcca acgccgacgg cacgcgcacg atcacctgca acgatggcac
     3181 gagcgtcgtg gtcaccaacg cggtcgtcga ctaccaggtg atgacctccg acgagctcaa
     3241 ggcctccgcg atggtcgcct cgatcaagtc ggtgacgatc ccgaccaccg gcccggccgc
     3301 cggccacccg ctcgtcacca tcaaggtgac cgagcgccac gggctcggcg tgaagggcct
     3361 ggttccggtc cagcccgacc cgttacatcc caacccgctg ttgccggtca gctggcggta
     3421 cgcgctgctc aagctcgaca ccggcgtcaa cggcagcgtc gacgagacct gggtcagcta
     3481 catggcgaac aacagcacca gcacggcgag cgccgagacc gccgcggcgg cggggttcac
     3541 cgacaacggc gacggcacgt acacctacaa gctcgccaag gacgtcaccg gcggcatctc
     3601 ggcggcgggc acgacctacg agccggacaa gccgcaccgc ctggtgatcc tgatggcggg
     3661 gcccggcaac ccgttcagcc cgatcaacct ggtcaaggac ttcgtgccga ccaccggcgc
     3721 ggacatcacc agctcgcacg agaagttcga cggcgcggcc tgcctcgagt gccacacctc
     3781 gttccgcgcg atcgccgacg gcaccggcgc gttcggcacc ggcgagttcc acagcggcgg
     3841 ccgctatgac acccggacct gcgtggcgtg ccacaacgac cagcgccggt ttgccaagcc
     3901 catcgtggac gacgcggcga tcggggccga cggcacgtgg accggcaacc tcgcggtggt
     3961 caacggcgag gcgacgatca acttcccggt gttcatccac aagatccaca tgggcgacaa
     4021 gctcgcgctc acgggcggca cgtactccgg tatcccgcag ccctacgaga ccacctatcc
     4081 gcaggacatc cgcaactgca ccaagtgcca ccgcaacccg gcgccgaaca acccggcgcc
     4141 gctggccgcc aactggaagg tgcccagccg gcgcgcctgc ggcgcttgcc acgacgacaa
     4201 gagcttcgtc aacccgccgc ccgccggacg cacgctgcac tcgggcggcg cgatggccaa
     4261 cgacaacaac tgcctcttgt gccacggtcc cggcggcatc ggccacgcgc ccgacgagat
     4321 ccacgcgccg gtctcgcccc cgaacccgaa caacatctac ctcaacccga ccagcggcaa
     4381 ctccaacacc aacgcggcct acgtcgcggc cgccgggttc gtcccgccgg gcgccaagca
     4441 gctgacctac gaggtcagcg ccgtcacgct cgatggctcg cgccacccgc agatcgtgtt
     4501 ccgcgtcctg aagggagatc cgaacgcgaa cccgccggtc gccccggccc cggtcacgtt
     4561 ccaggatcac acggccgcca ccgagatgat ccccggcttc gtcggctcgc ccagcgcgta
     4621 cttcgtctac gcggtcccgc aggacaacat caccgcgccg gcggacttca acgtctcggt
     4681 gagcggctac ctccgcagca tctggaacgg gacggcgacc ggcaacggag ccggcacgat
     4741 gaccggcccc gacgcaaacg gcttctacac cgtcacgctg accagcgacg cgatcaaggt
     4801 cccgaccagc gcggtcatgc tgaccggcgg catcggctac acctacagcc ttggctcggc
     4861 cacgccgccc tcgggcccgc cgttcagcaa caacacgcag ccgttcaccg agatcgacat
     4921 gccgtcgaag tacccgtaca cgccgaacgc cagcggcttc gccggcaagg gcggcctgat
     4981 cgtcccgccg cccgacgtct cgaaggtcgc cgacaagccc ttcaccgccc ggcgcgcgat
     5041 cgtcgacaac tccaagtgcg acacctgcca cgtcagcctc ggcgtcggcc ctgacttcca
     5101 cgccggccag cgcaacgact cggcgacctg cgcgtggtgc caccggccga accagaccag
     5161 cagcgggtgg gccgcgaacg ccaaggactt catccacagc atccacgccg cggagaagcg
     5221 caccgtcccg ttcaactggc acgccgagtc accgaccgag ggcttcttca acgtcaccta
     5281 cccggcgatc ttgaaccgct gcgagatgtg ccacctgccg ggcacgttcg acttcagcct
     5341 gacgtcgacc accagcgcgc tgccgaacat gctgccgagc acggtcggcc agggccgcta
     5401 cgacgtcaaa ccgaccagca atccgagcgg cttcttcgcg atcgcgccgc cgcagtacgt
     5461 gacgcggaac aactcggcgt tcgactacgg gttcgggttc aacacctcca acgtgaacgc
     5521 gacgctgccc gacgggatca gcggtacgca gggagcgacc agctgcacgc cggagcagcc
     5581 gtgtctgtgc accgcggcga atccgtgcag cgcgacgatc tccgggacct acgccgtcaa
     5641 caacgtgcag gtgagcttca cccagaagat cagcggcgtc acgaacccgt gcaacgcgag
     5701 cacgccgtgc acctgcacga ccgcccagcc gtgcaccggt gtggtcgcga cctgcacccc
     5761 gaccacccca tgccaggcgc agcccacgac gctggtgaac tcgccgatcg cggcggcctg
     5821 cgtggcgtgc cacgactctc cgtcggcggt cgaccacatg cagaccaacg gcgcctcgat
     5881 ctgggagccg cgctcgatcg cgctgaacaa gccgcagaag gaagagtgcc tgatctgcca
     5941 cggcccgaac cggatcgccg gcatcgcgct ggtgcacacc gacaagacgc cgtagcgcgg
     6001 atccgtcccg cccgcgccgg tgcgcccgcg aggcgagccg gtgacgggcg aacccgcggt
     6061 gcctggccgg cgccgcgttc gtcgtttcgg gtcagcgcgg cggaccggcg ggcggcgcgc
     6121 cgggcgagac actggctagc ttgtcgagat caatcgccgc catggacccg atcgcaagcg
     6181 cggat
//
LOCUS       DASVOR010001329         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_71957,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001329 DASVOR010000000
VERSION     DASVOR010001329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71957"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..1117)
                     /locus_tag="VF516_25375"
     CDS             complement(104..1117)
                     /locus_tag="VF516_25375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111096.1"
                     /translation="MLARCMISAAVVWTGCSFEGNTPDSGTVTVHGQVVDFQTGLAVD
                     TTTDVTVSGLIPPPQVDRQGASFTLAGVLENSAFEVLATAAMHRPTYSPVVVTASGID
                     GLKAPVVGEAFITALATAFGTAPSPAKGIVLLHLVDAMGRPQSGVAASDFAIAGSTGP
                     RFLDGSMMAAVAATTSSTSGWAVFFDVPPGLVGLTQGPSSATTIDMPLLPSAAGAVTI
                     AEGKVTRGAPVLPSNVSFAAQIVPIFSARGCVACHSGNGPGKDQGGLTLAGSDNLIYH
                     ELVEERPGIRVNLAMPEKSLVLTMPSAEDPPDAHPTIVFTGPRDPDYLKLLVWIREGA
                     KQN"
     gene            complement(1277..>2278)
                     /locus_tag="VF516_25380"
     CDS             complement(1277..>2278)
                     /locus_tag="VF516_25380"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol lysyltransferase
                     domain-containing protein"
                     /protein_id="HEX8111097.1"
                     /translation="VLRDSRSLREQLRRAQAKGVAVRTVGPAELQPGQPLRLALERLI
                     ARWLASRQIAPMGFLVHVDPFTFPDQRRYFVAERDGAVVGFLSAIPIYARPGWFFEDF
                     LRDHDAPNGTVELLIDAGMRAAAGEGVPYVTLGLVPLSGEVSPWLRAARRWGKALYDF
                     DGLRAFKAKLKPRAWDPIFLSYPAGASGFVAMFDTLTAFSRGGLLRFGLETLLRGPAI
                     VMRVLAALLLVWTALLALPGAARWFPSSAWQWGWVGFDLVLAVSLLTLSYRWRAVLAD
                     LVATAVTADAVLTLGQAIAYDLPRREGPLDAAVAVIAVAAPTVASVLLWNARAHRTR"
BASE COUNT          337 a          837 c          826 g          278 t
ORIGIN      
        1 tgccctgctg tcgccggaga tcgccgcgac cgccggggtc cgggtcgccc ggcgggacct
       61 gttcctgacg agcctggtca agcgcccggc atgacgcgcg cggtcagttc tgcttcgcgc
      121 cctcgcggat ccacaccagc agcttgaggt agtcgggatc gcgcgggccg gtgaacacga
      181 tcgtgggatg agcgtccggc gggtcctcgg ccgacggcat cgtcagcacc agactcttct
      241 cgggcatcgc gaggttgacc cggatgcccg gccgctcctc gacgagctcg tggtagatca
      301 ggttgtcgct gccggcgagc gtcagcccgc cctggtcctt gccgggcccg ttcccggagt
      361 ggcaggcgac gcagccgcgc gccgagaaga tcggcacgat ctgcgccgcg aacgacacgt
      421 tgctcggcaa caccggcgca ccgcgtgtga ccttgccctc ggcgatcgtc acggcccccg
      481 ccgccgacgg cagcaacggc atgtcgatgg tggtcgccga cgacggcccc tgggtgagcc
      541 cgaccagccc gggcggcacg tcgaagaaca cggcccagcc cgaggtcgag ctcgtggtcg
      601 cggcgaccgc cgccatcatg ctgccatcga ggaaccgcgg cccggtgctg ccggcgatcg
      661 cgaagtcgct cgccgcgacc ccggactgcg gccgccccat cgcgtcgacc aggtggagca
      721 gcacgatgcc cttggccggc gacggcgccg tgccgaacgc ggtggccagg gcggtgatga
      781 acgcctcgcc gaccaccggc gccttgaggc cgtcgatgcc ggacgccgtc accacgaccg
      841 ggctgtaggt cgggcggtgc atcgccgcgg tggcgagcac ctcgaacgcc gagttctcga
      901 ggacgccggc gagcgtgaac gatgcgccct ggcggtcgac ctgcggcggc gggatcaacc
      961 ccgacaccgt cacgtcggtg gtcgtgtcga ccgccaggcc agtctgaaag tcgacgacct
     1021 gtccgtggac cgtgaccgtg ccgctgtcgg gcgtgttgcc ctcgaacgag catccggtcc
     1081 agacgaccgc ggccgagatc atgcagcgag ccagcatgcg tgctcctttc ccggtccagt
     1141 gcttgtggga cagctgtcgg tggtggtcgc aagtttcgtc cgctgtgcgc caggtcgcat
     1201 ccgacgctcg gcacctcgcc gcgccggcgt gcggcgcgcc acagccgcgc gctcgaccgt
     1261 acgcggcgtg cacagctcac cgggtgcggt gggcccgggc gttccataac aggaccgacg
     1321 cgacggtcgg cgcggcgacc gcgatcaccg cgaccgcggc gtcgagcggc ccctcccggc
     1381 gcggcaggtc ataggcgatc gcctgcccca gggtcagcac ggcgtcggcg gtgaccgcgg
     1441 tcgcgaccag gtcggcgagc accgcccgcc agcggtagct cagggtgagc agggagaccg
     1501 ccagcacgag gtcgaagccg acccagcccc actgccacgc ggacgacggg aaccagcgcg
     1561 ccgcgccggg cagcgccagc agcgccgtcc acaccagcaa gagcgcggcg agcacgcgca
     1621 tgacgatcgc cggcccgcgc agcagggtct cgagcccgaa ccggagcagg ccgccgcgcg
     1681 agaacgcggt gagcgtgtcg aacatcgcga cgaacccgct tgcccccgcc gggtacgaca
     1741 ggaagatcgg atcccacgcc cgcggcttga gcttggcctt gaacgcgcgc agaccgtcga
     1801 agtcgtacag ggccttgccc cagcgccgcg ccgcgcgcag ccacgggctg acctcgccgg
     1861 acagcgggac caggccgagc gtcacgtacg ggacgccctc gccggccgcc gcccgcatgc
     1921 cggcgtcgat cagcagctcg accgtaccgt tgggggcgtc gtggtcgcgc aggaagtcct
     1981 cgaagaacca gcccgggcgg gcgtagatcg ggatcgcgct caggaacccg acgaccgcgc
     2041 cgtcgcgctc ggcgacgaag tagcggcgct ggtcggggaa cgtgaacgga tcgacgtgga
     2101 cgaggaaccc catcggcgcg atctgccgcg acgccagcca gcgcgcgatc agccgctcga
     2161 gcgcgagccg gagcggctgg cccggctgga gctcggccgg accgaccgtc cgcaccgcga
     2221 cgcccttggc ctgggcgcgg cgcagctgct cgcgcaggct gcggctgtcg cgcaggac
//
LOCUS       DASVOR010001330         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_159503,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001330 DASVOR010000000
VERSION     DASVOR010001330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159503"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="VF516_25385"
     CDS             complement(<1..868)
                     /locus_tag="VF516_25385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111098.1"
                     /translation="MAADRRRRILRFLGPHGDLLAAESPGLTVYDASGHVRIRSEIEF
                     LDIAPVGDELWVAASHHLIRLSARDGRERARDRIDYLDPDGRFLQSSTAPQLPIWHGA
                     QPVVIRTDPVRTEVPGPGGDVILPISDGRWLLWQGGQLRLWRSIGEAWRKPIGERGTR
                     PQDAQLVLDGRLFALTQHRSPGNDGELRLTVAAVSDGAQHTQLRLPAVTQLLFAARRG
                     VAIARAGDRLSLIDLRFGRWIRDLVLPAGVTELAVDDGLQWFALAGADGLEVVRPDAL
                     TTAGARAAGTTGA"
     gene            1053..>1806
                     /locus_tag="VF516_25390"
     CDS             1053..>1806
                     /locus_tag="VF516_25390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111099.1"
                     /translation="MEYAVKDKLARLEEERLDEERERRRKQRARDALQRIDAVGKQTL
                     LLLRENALQKPGRAEGEEGKDDNPEPGRLSRVLFEAAVTADDPRRAARRNRHANRSLA
                     QRAEEQATRRRIDQAMQTIRNAVTGTASTGLKEGLESAARQVNDVVAQVSTFARGAMG
                     MPDIPAQPLGELVTEERAETEATRPGATATLDPTPDPTLGRTREGDPTAAFAPRGQLG
                     ERGFEMTLELGQPPRDHAGDASPGPGRDAMDPL"
BASE COUNT          272 a          651 c          686 g          197 t
ORIGIN      
        1 tcgccccggt cgtgcccgcg gcgcgcgcac cggccgtcgt cagcgcgtcg ggacggacca
       61 cctccaggcc gtcggcgccg gcgagcgcga accactgcag gccgtcgtcg accgcgagct
      121 cggtgacgcc ggcgggcagc accaggtcgc ggatccagcg gccgaaccgc aggtcgatca
      181 ggctcagccg atcgccggcg cgcgcgatcg cgacgccgcg gcgagccgcg aacaggagct
      241 gggtcaccgc cgggagccgg agctgggtgt gctgtgcgcc gtcggacacc gcggcgacgg
      301 tcagccgcag ctcgccgtcg ttgccggggc tgcggtgctg ggtcagcgcg aacagccggc
      361 cgtcgagcac gagctgcgcg tcctggggcc gcgtgccgcg ctctccgatc ggcttgcgcc
      421 acgcctcgcc gatgctgcgc cacagccgga gctggccgcc ctgccacagc agccaccgcc
      481 cgtcgctgat cggcaggatc acgtcgccgc cggggccggg gacctcggtg cgcaccggat
      541 cggtgcggat cacgaccggc tgcgcgccgt gccagatcgg cagctggggc gccgtgctcg
      601 actgcaggaa ccggccgtcc ggatccaggt agtcgatccg atcgcgcgcg cgctcgcggc
      661 catcgcgcgc cgacagccgg atcaggtggt gcgacgcggc gacccagagc tcgtcgccga
      721 ccggggcgat gtccaggaac tcgatctcgc tgcggatgcg gacatgtccc gacgcgtcat
      781 acacggtgag gccgggcgac tctgcggcca ggagatctcc gtgcggtccc aggaaccgca
      841 agatgcgccg ccgtcggtcg gctgccactg cggcgccatg ctaccgcgat ccggtcgttg
      901 ccatccccgg atgtgtgtga tctatcaccg aagccgggag aggtgtcacc gaagacagga
      961 gatcgggccg cggggacagg acatgtaagc catggttgcc gtgcggtccg gcgggagaac
     1021 tccggcgcgg ccgtagtacg gcggagcccg ccatggagta cgccgtcaaa gacaagctcg
     1081 cgcgcctcga ggaggaacgg ctcgatgagg agcgggagcg ccggcgcaag cagcgcgccc
     1141 gtgacgcgct gcagcggatc gacgcggtcg gcaagcagac gctcttgctg ctccgggaga
     1201 acgcacttca gaagccggga cgggcagagg gcgaggaggg caaggacgac aaccccgagc
     1261 cgggccggct cagccgggtg ctgttcgaag ccgcggtgac ggcggacgat ccccggcggg
     1321 cggcgcgccg caaccggcac gccaaccgca gcctggcgca gcgcgccgag gagcaggcca
     1381 cccgccgccg gatcgaccag gcgatgcaga cgatccgcaa cgcggtcacc ggcaccgcga
     1441 gcaccgggct caaggaaggg ctcgagagcg ccgcgcgcca ggtcaacgac gtcgtcgcgc
     1501 aggtctcgac attcgcccgc ggcgcgatgg gcatgccgga catcccggcc cagccgctcg
     1561 gggagctggt cacggaggag cgcgcggaga ccgaggcgac ccggccgggc gcgacggcca
     1621 cgctcgatcc cacgcccgat cccacgctcg ggcgcacgcg cgagggcgat ccgaccgcgg
     1681 cgttcgcgcc gcgtggccag ctgggcgagc gcggcttcga gatgacgctc gagctcggtc
     1741 agccgccgcg cgatcacgcg ggcgacgcgt cgccgggccc cgggcgtgac gccatggacc
     1801 cgctgc
//
LOCUS       DASVOR010001331         5659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43171,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001331 DASVOR010000000
VERSION     DASVOR010001331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43171"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VF516_25395"
     CDS             complement(<1..215)
                     /locus_tag="VF516_25395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111100.1"
                     /translation="MMAVLGAGCMTGAGEPTAAQGSPLTGDCDAFMCGTNSPQIAEFG
                     FWELKLPTTVDVPGARNNVGLRVLRFE"
     gene            complement(388..999)
                     /locus_tag="VF516_25400"
     CDS             complement(388..999)
                     /locus_tag="VF516_25400"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8111101.1"
                     /translation="MTEPSDLTLLDRWRDGDAAAGEALFLRHFDSIYRFFETKWDAHV
                     DDLVQSTFLNCLRARDQFRKESSFRTYLFTIARHELYGALRGRLRDGERLDFELSSIA
                     ELVSTPGTRLARDQEHRQLIDALRQLPIEQQTLLELHYWDDLDIAALAEVFEAPQPTI
                     RTRLHRARKALREKMARVAPRRAFDTLESMDTWARGLSQRAAR"
     gene            complement(1043..1468)
                     /locus_tag="VF516_25405"
     CDS             complement(1043..1468)
                     /locus_tag="VF516_25405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111102.1"
                     /translation="MHADLRTGLPWQRLPAPRPGPERRRIGFRTCGRPCERHEVALRD
                     RVGLVHPVVVDVGCRNTVFNAQAQSAASLVPELSARGVWRYRVELVRESAEETARVYT
                     AYAQLVAGEVAPREAVRRAAVHEQFGVTRGTMRTLTVLR"
     gene            1547..4696
                     /locus_tag="VF516_25410"
     CDS             1547..4696
                     /locus_tag="VF516_25410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111103.1"
                     /translation="MSVSAFTRLLSTRPVPARRTPAAAPPRRRATRAPGLWLSVLALA
                     GATLGCGHGELPGAPDAGVDPVAFAPGQGSVHGYVDATTGTVFALPDATVQLKRVTDN
                     ALTPAVTTDVHGAFTASGVPAGTYVICLSGTPGFSNACSSTSFAVTAGKIAYPAHAVF
                     SPLRVAVWGRVRLSDSTDVRYENSLFGKQVDTFVKALTTGGTLAAGPVRANGRGQYVL
                     RQLAPNTTYHLVVQSETTVVDTLITTGTAPSKQDVTLPNRRPVVAEVDALQGGAGVRH
                     VAAGSVVTVTASASDPDGNALHYQWLAPNGGSCPTTDAATVDCTMPTTLGVQSIYVQV
                     SDGAGEYAVGRVRVEVGPAISLFTGKVITDGNAVVPGAEIRVNGAATTTSAAGAFSLT
                     VPEASRYVLTIKKDGFQMISKVFLAERVGATYQLRKADAVVIDPTVDNTVLIHAPKTD
                     TGGQAYQDVSLTLHANSIIDSAGNKVTTPVTVYSSRFDHLFDTFDRMPGDYAGKTSGG
                     SDTTLTSYGAVEVNLRGPGGAKYNLAAGMPSDLKYPVHPSQLASAPPTLPIWYYNEDT
                     GIWEEDGVATLSSGAYLGQAKHFTAINVDLAKQNATCVKLVVDQTTLAVPLKIRLSVP
                     GFPDRDRDVTENVSAIVRLPPNVANSKIVVLDGANQPIPNSTRIFTTGNVLPDGTNLS
                     LQAPYNVCITPPSPPVTLGLSLPQNPDPYWLTKKINPGADDAARSAYADQYYMAIQAD
                     TTLGQWRSRNGFAADDSADDAKAFYLNAGDLEFGRSMHMKNRSDGGIAYYVTNFADAD
                     KAFAGNPTDVIATVAMEYSQYPSMVPGAPRFTKFYVFDKAGNRVNRAELDNRGDKYVP
                     GLCVVCHGGTLPINIATASPPGNTDSRFIPFDLKSFASSPLLPGFPALLDRTAQEENF
                     RKLNQGVYQLTSPTDAEKAVIDAWYFPNGVTAPMQTQQDGNIPFNWNGNNTDAQFYKD
                     VVAVSCRSCHTSRQNPLDFGDPLSFQGQPIQFAVCDSGQMPQSFVAWRNFWHSTSPHE
                     ATSIETYLNLGAGTCVGPQ"
     gene            4769..>5659
                     /locus_tag="VF516_25415"
     CDS             4769..>5659
                     /locus_tag="VF516_25415"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111104.1"
                     /translation="MPSREREELSPAGGHAGTATPERADTTSPEAAVESTSRIGERPE
                     ADRLVLAVARRRIANRMFAKDEQVHLGRYHLLEMVGAGGMGVVWGAWDPQLERRVAIK
                     LVKATVPAARQRIEREGQALARLSHPNVVTVHDVGVVDDQIYIVMEWVRGQNLRAYCR
                     TRRTLREIVAIYRAAGDGLAAAHAAGLIHRDFKPDNAIRGDDGRVRVLDFGLARDDLG
                     GADSEAGSSGLTRGAGTPRYMPPEQAEGRALTPAVDQYAFCVSLREALAGRNADGKDA
                     DIPGWIEAILVRGTARDPGER"
BASE COUNT          888 a         2055 c         1911 g          805 t
ORIGIN      
        1 ttctcgaacc gcagcacccg cagccccacg ttgttgcgcg cgcctggcac atcgacggtc
       61 gtggggagct tcagctccca gaatccgaac tcggcgatct gcggtgagtt ggtgccgcac
      121 atgaacgcgt cgcaatcgcc ggtgagcggc gaaccctggg cggcggtggg ctcgccggcg
      181 ccggtcatgc aacccgcgcc gagcaccgcc atcaccagac tggatctacg gacagacatt
      241 ctttcacctc tcgcccggtg gatctctcgg gcgctctggt agatgcagga ccgacgagtt
      301 cgtgccggtc ccgcccgatg aaatcgacct gtcgctccaa tcagccgaaa ccgctgacgg
      361 ttccggggtg tggagccctg gcagcgttta cctggcggcg cgctgcgaca gcccgcgcgc
      421 ccaggtgtcc atcgactcca gcgtatcgaa cgcccgccgg ggtgccacgc gggccatctt
      481 ctcgcgcagc gccttgcggg cgcggtgcag ccgggtccgg atcgtcggct gcggggcctc
      541 gaacacctcg gcgagcgcgg cgatgtcgag gtcgtcccag tagtgcagct ccagcagcgt
      601 ctgctgctcg atcgggagct ggcgcagcgc atcgatcagc tggcggtgct cctggtcgcg
      661 cgcgagccgg gtgcccggcg tcgacaccag ctcggcgatc gacgacagct cgaagtccag
      721 ccgttcgccg tcgcgcagcc ggccgcgcaa cgcgccatac agctcgtgcc gggcgatcgt
      781 gaacaggtag gtccggaagc tcgactcctt gcggaactgg tcccgcgcgc gcaggcagtt
      841 caggaacgtc gactggacca ggtcatcgac gtgggcatcc cacttggtct cgaagaaccg
      901 gtagatgctg tcgaagtggc gcaggaacag cgcctcgccc gccgcggcgt ccccatcacg
      961 ccagcgatcg agcagggtca ggtccgatgg ctcggtcatc gaccggtcgc gagtgtatct
     1021 gctttctgtc ggatcgcgcg ggtcagcgca gcacggtcag cgtccgcatc gtgccgcggg
     1081 tcacgccgaa ctgctcgtgg acggcggcgc gccggaccgc ctcgcggggc gcgacctcgc
     1141 cggcgacgag ctgggcgtag gcggtgtaga cgcgggcggt ctcctcggcg gactcgcgca
     1201 cgagctcgac ccggtaccgc cacacgccgc gcgcggagag ctcgggcacc aggctcgcgg
     1261 cgctctgggc ctgggcgttg aacacggtgt tgcggcagcc gacgtcgacc accaccgggt
     1321 ggaccagccc gacccggtcg cgcagcgcga cctcgtggcg ctcgcacggc cggccgcagg
     1381 tccggaagcc gatccggcgg cgctccgggc ctgggcgcgg cgccggcaat cgttgccacg
     1441 ggaggccggt gcgcagatcg gcgtgcatga tgcgaaatcg tgaaacgcgc ggcgggcgcc
     1501 tggctctacc tgccccggag ccgggattgc tcccgaggag gtctccatgt ccgtttctgc
     1561 gttcaccagg ttgctttcca cacgtccggt gcctgcgcgc cgtacgcccg ccgccgcgcc
     1621 gccgcgccgc agggccacgc gagctcccgg tctctggctg tctgtcctcg cgcttgccgg
     1681 cgcgacgctc ggctgcggtc acggcgagct ccccggagca cccgatgccg gcgtcgatcc
     1741 cgtcgcgttc gcccccggcc agggctcggt gcacggctac gtcgacgcca cgaccgggac
     1801 ggtgttcgcg ttgcccgatg ccaccgttca gctcaagcgg gtgaccgaca acgcgctgac
     1861 cccggcggtc accaccgacg tccacggcgc gttcaccgcg tccggcgttc cggccggcac
     1921 ctacgtgatc tgcctgtcgg ggacgccggg gttctcgaac gcctgctcgt cgaccagctt
     1981 cgcggtcacc gccgggaaga tcgcctaccc cgcgcacgcc gtgttcagcc cgctgcgcgt
     2041 cgcggtgtgg ggccgggtgc ggctgagcga cagcaccgac gtacgctacg agaactcgct
     2101 gttcggcaag caggtcgata ccttcgtcaa ggcgctgacc accggcggca cgctcgccgc
     2161 cggtccggtg cgggccaacg gccgtggcca gtacgtgctg cgccagctgg caccgaacac
     2221 cacctatcac ctcgtggtgc agagcgagac cacggtggtc gacaccctga tcaccaccgg
     2281 gaccgcgccg tccaagcagg acgtcacgct gcccaaccgc cgcccggtgg tcgccgaggt
     2341 cgacgcgctg cagggcggcg cgggcgtccg ccacgtcgcg gcgggatcgg tcgtgaccgt
     2401 caccgcgagc gcctccgatc cggacggcaa cgcgctgcac taccagtggc tggcacctaa
     2461 cggcggcagc tgcccgacca ccgacgccgc gaccgtggat tgcacgatgc cgaccaccct
     2521 gggtgtgcag tcgatctacg tccaggtctc cgacggcgcg ggcgagtacg cggtgggccg
     2581 cgtccgcgtc gaggtcggcc cggcgatctc gctgttcacc ggcaaggtga tcaccgacgg
     2641 caacgcggtg gtccccggcg ccgagatccg ggtcaacggc gcggccacca ccaccagcgc
     2701 ggccggcgcg ttctcgctga ccgtgccgga ggccagtcgc tacgtgctga cgatcaagaa
     2761 ggatgggttc cagatgatct cgaaggtctt cctggccgaa cgggtcgggg ccacgtacca
     2821 gctgcgcaag gccgacgccg tggtgatcga tccgacggtc gacaacaccg tgctgatcca
     2881 cgctcccaag accgataccg gcggccaggc gtaccaggac gtctcgctca cgctccatgc
     2941 caactcgatc atcgacagcg ctggcaacaa ggtgaccacg ccggtcacgg tctatagctc
     3001 gcggttcgat cacctgttcg acaccttcga tcgcatgccc ggtgattacg ccggcaagac
     3061 cagcggcggc agcgacacca cgctgaccag ctacggcgcg gtcgaggtca acctgcgcgg
     3121 ccccggtggc gcgaagtaca acctcgcggc cggcatgccg tcggatctca agtacccggt
     3181 gcacccgtcg cagctcgcga gcgcgccgcc gacgctcccg atctggtact ataacgagga
     3241 caccggcatc tgggaggagg acggcgtcgc gacgctgtcc agcggcgcgt acctcggtca
     3301 ggccaagcac ttcaccgcga tcaacgtcga cctcgcgaag cagaacgcca cgtgcgtcaa
     3361 gctggtcgtc gatcagacca cgctggccgt gccgctcaag atccggctgt cggtccccgg
     3421 cttccccgat cgggatcgcg acgtcaccga gaacgtctcg gccatcgtcc ggctgccccc
     3481 caacgtggcc aactccaaga tcgtggtgct cgacggcgcc aaccagccga tccccaactc
     3541 gacgcggatc ttcaccaccg gcaacgtcct gcccgacggc accaacctca gcctgcaggc
     3601 gccgtacaac gtctgcatca ccccgccgtc gccgccggtc acgctcggcc tcagcctgcc
     3661 gcagaacccc gacccgtact ggctgaccaa gaagatcaac ccgggcgccg acgacgccgc
     3721 ccgcagcgcc tacgccgacc agtactacat ggcgatccag gccgacacca cgctcggcca
     3781 gtggaggagc cgcaacgggt tcgccgccga cgacagcgcc gacgacgcca aggcgttcta
     3841 cctcaacgcc ggggatctgg agttcggccg cagcatgcac atgaagaacc gcagcgacgg
     3901 cggcatcgcg tactacgtga ccaacttcgc cgacgccgac aaggcgttcg ccggcaaccc
     3961 gacggacgtg atcgccacgg tcgcgatgga gtactcccag tacccatcga tggtcccggg
     4021 cgcgccgcgg ttcaccaagt tctacgtgtt cgacaaggcc ggcaaccggg tcaaccgcgc
     4081 cgagctcgac aaccgcggcg acaagtacgt tcccgggctg tgcgtcgtgt gccacggcgg
     4141 cacgctgccg atcaacatcg ccacggcgtc gcccccgggc aacaccgact cgcggttcat
     4201 cccgttcgac ctgaagtcgt tcgcctccag cccgctgctc cccgggttcc cggcgctgct
     4261 ggatcgcacg gcgcaggagg agaacttccg caagctgaac cagggggtct accagctcac
     4321 ctcgcccacc gacgccgaga aggcggtgat cgacgcgtgg tactttccga acggcgtcac
     4381 cgctccgatg cagacgcagc aggacggcaa catcccgttc aactggaatg gcaataacac
     4441 ggacgcgcag ttctacaagg acgtggtcgc cgtgtcgtgc cggtcgtgcc acacgtcgcg
     4501 ccagaatccg ctcgacttcg gcgatcccct gtcgttccag ggccagccga tccagtttgc
     4561 ggtctgcgac agcgggcaga tgccgcagtc gttcgtggcg tggcgcaact tctggcacag
     4621 cacctcgccg cacgaggcca cgagcatcga gacctacctg aacctcggcg ccggaacgtg
     4681 tgtgggaccg caatgacgtg aaccgccgtg gtgcgcccgc cgccgtggat cgggcctgca
     4741 ggtccgggcc gatgatatcc tgccggtgat gccgtcgcgc gaacgcgagg agctctcgcc
     4801 ggcagggggg catgccggca ccgcgacccc ggagcgcgcc gacaccacga gcccggaggc
     4861 cgcggtggag tccacctcgc ggatcggcga gcggcccgag gccgatcggc tcgtcctcgc
     4921 ggttgcccgc aggcggatcg cgaacaggat gttcgccaag gacgagcagg ttcacctcgg
     4981 gcgctaccac ctgctggaga tggtcggcgc cggcggcatg ggcgtggtgt ggggcgcctg
     5041 ggatccacag ctggagcgcc gggtcgcgat caagctggtc aaggcgacgg tgccggccgc
     5101 gcgccagcgg atcgagcggg agggccaagc gctggccagg ctatcgcacc ccaacgtggt
     5161 caccgtccac gacgtcggtg tggtcgacga ccagatctac atcgtgatgg agtgggtgcg
     5221 cggccagaac ctgcgcgcgt actgccgcac gcggcgcacg ctgcgcgaga tcgtcgcgat
     5281 ctaccgcgcc gccggcgacg ggctcgccgc ggcccacgcc gcggggctga tccaccgcga
     5341 cttcaagccc gacaacgcga tccgcggcga tgacggccgg gtccgcgtgc tcgacttcgg
     5401 gctcgcgcgc gacgatctgg gcggggcgga cagcgaggca gggagctcgg gcctgacccg
     5461 cggtgcgggc accccccgct acatgccgcc cgagcaggcc gagggccgcg cgctgactcc
     5521 cgcggtcgat cagtacgcgt tctgcgtctc cttgcgcgag gcgctcgccg ggcgcaacgc
     5581 cgacggcaag gacgccgaca tccccggctg gatcgaggcg atcctggtcc gcggcacggc
     5641 gcgcgatccg ggcgagcgg
//
LOCUS       DASVOR010001332         2386 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_306047,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001332 DASVOR010000000
VERSION     DASVOR010001332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2386
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_306047"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VF516_25420"
     CDS             complement(<1..1416)
                     /locus_tag="VF516_25420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8111105.1"
                     /translation="MVDVLDAFHPLVAGWFRERFGAPTEPQRAGWPRIGAHQDVLIAA
                     PTGSGKTLAAFLACMDELVRAGLHAPLGNHTAILYVSPLKALSNDVQRNLEAPLAELA
                     AYAAARGVKLPEIRVAVRTGDTPVGERAKLAKRPPHILVTTPESLYILLTAERGRAGL
                     AELRTVIVDEIHAIAGDKRGAHLALSLERLDRLVVQATGRAPVRVGLSATQKPIATIA
                     RLLVGTRRPLPHIVDAGHRRALDLAIELCDDELGAVASNEQFGRIYDRIAALVHQHRS
                     TIVFVNTRRLVERAAAALEQRLGEDHVVAHHGSMSRALRLAAEHKLKHGQVKCAVATA
                     SLELGIDVGTVDLVVQLGSPRSIATLLQRVGRSGHHLGGTPKGRLFALTRDQLVECAA
                     LVRGVRRGNLDTIALRDAPLDILAQQIVAAAAAEAIPEHELAHLIRGAAPYADLPDDK
                     LEQVLTMLSEGVSDRRGRAGAH"
     gene            complement(1465..2157)
                     /locus_tag="VF516_25425"
     CDS             complement(1465..2157)
                     /locus_tag="VF516_25425"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX8111106.1"
                     /translation="MSDPGLRLCVSFGTNPMAQDLATPAHAIVEATGVEPLANIALPI
                     LGRVVNTAGTFTASSQLRFQENPDFDVDDLTIYLWMSPQSKPAQNGGYFLLDNNTEYY
                     ATYDGDATVRCGVAGTSVNSHATVAPGTWHHVACTYGKADRLLRVYVDGNLGGCQMAS
                     AVPHGGADGVAIGANYSAKNGFQQNFLGNLGDLHLYGTELAPDQICRAAGRTGCNFVC
                     PGDGGGDPGSGR"
BASE COUNT          320 a          811 c          929 g          326 t
ORIGIN      
        1 gtgggccccg gcccggccgc ggcgatccga cacgccctcg gacagcatgg tcagcacctg
       61 ctcgagcttg tcgtcgggca ggtcggcata cggcgcggcg ccgcggatca ggtgcgcgag
      121 ctcgtgctcg gggatcgcct cggcggccgc ggcggcgacg atctgctggg cgaggatgtc
      181 gagcggcgcg tcgcgcagcg cgatggtgtc gaggttgccg cgccggacgc cgcggaccag
      241 cgcggcgcac tcgacgagct ggtcgcgggt cagcgcgaac agccggccct tgggcgtgcc
      301 gccgaggtgg tgaccggacc ggccgacccg ctggagcagg gtcgcgatcg agcgcggcga
      361 gccgagctgg accacgaggt cgaccgtgcc gacgtcgatg ccgagctcga gcgacgcggt
      421 ggcgaccgcg cacttgacct ggccgtgctt gagcttgtgc tcggcggcga gccgcagcgc
      481 gcgcgacatc gagccgtggt gggccacgac gtggtcctcg ccgagccgct gctcgagggc
      541 cgcggcggcg cgctcgacca gccgccgggt gttgacgaac acgatcgtcg agcggtgctg
      601 gtggaccagc gcggcgatcc ggtcgtagat ccgcccgaac tgctcgttgg acgcgaccgc
      661 gccgagctcg tcgtcgcaca gctcgatcgc gaggtcgagc gcgcggcggt ggccggcgtc
      721 gacgatgtgg ggcagcggcc gccgggtgcc gacgaggagc cgggcgatcg tggcgatcgg
      781 cttctgggtc gcggacagcc cgacgcggac cggcgcgcgc ccggtggcct gcacgaccag
      841 gcgatcgagc cgctcgagcg acagcgcgag gtgggcgccg cgcttgtcgc cggcgatcgc
      901 gtggatctcg tcgacgatca cggtgcggag ctcggcgagc ccggcgcggc cgcgctcggc
      961 ggtcagcagg atgtacaggg actccggcgt cgtgaccagg atgtgcggcg gccgcttggc
     1021 cagcttggcg cgctcgccga ccggggtgtc tccggtgcgg accgcgacgc ggatctcggg
     1081 cagcttgacc ccgcgcgccg cggcgtaggc cgcgagctcg gcgagcggtg cctcgaggtt
     1141 gcgctggacg tcgttggaca gcgccttgag cggagagacg tacaggatcg ccgtatggtt
     1201 gccgagcggg gcgtggagcc cggcgcgcac cagctcgtcc atgcaggcca ggaacgcggc
     1261 cagggtcttg cccgatccgg tcggcgcggc gatcaggacg tcctggtgcg cgccgatccg
     1321 gggccagccg gcccgctgcg gctcggtcgg tgcgccgaac cgctcgcgga accagcccgc
     1381 gaccagcggg tgaaacgcgt cgaggacgtc gaccacggca ccacagcgta gcgcacggcg
     1441 ccgcgggcgg atacccggcc ggcgctaccg gccgctgccg ggatctcccc ctccgtctcc
     1501 ggggcagacg aagttgcagc cggtccggcc ggcggcgcgg cagatctggt ccggggcgag
     1561 ctcggtccca tagaggtgca ggtcgccgag gttgccgagg aagttctgct ggaacccgtt
     1621 cttggcgctg tagttggcgc cgatcgcgac gccgtccgcg ccgccgtgcg ggacggcgga
     1681 tgccatctgg cagccgccga ggttgccgtc gacgtacacc cgcagcaggc ggtcggcctt
     1741 gccgtaggtg caggcgacgt ggtgccaggt gccgggagcc accgtcgcat ggctgttgac
     1801 cgacgtgccg gcgaccccgc atcgcacggt ggcgtcgccg tcgtaggtgg cgtagtactc
     1861 ggtgttgttg tcgaggagga agtagccgcc gttctgcgcc ggcttgctct gcggggacat
     1921 ccagaggtag atcgtcaggt cgtcgacgtc gaagtccggg ttctcctgga agcgaagctg
     1981 cgagctcgcg gtgaatgtgc cggcggtgtt gaccacgcgg cccaggatcg gcagcgcgat
     2041 gttggcgagc ggctcgacgc cggtggcctc gacgatcgca tgcgccggcg tcgcgaggtc
     2101 ctgcgccatc gggttggtgc cgaacgacac gcagagccgc aggccggggt cgctcacatc
     2161 gcacttcccc gacgaggtcc ccgaggttcc ggagccaccg gatccagacc ccggtccccc
     2221 ggagccgcca cccggatcgt cggcagagct ggccggagag gagaacccac aacccgcgat
     2281 caaccacacg aggcatgcgt agcgcatggg ccgagcttcc cacgccgcgc gcgcgacttc
     2341 gctcaaacct cctgacgccg gaggccccag acggtcgcgt agtgat
//
LOCUS       DASVOR010001333         2275 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43187,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001333 DASVOR010000000
VERSION     DASVOR010001333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2275)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2275)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2275
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43187"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="VF516_25430"
     CDS             <1..314
                     /locus_tag="VF516_25430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8111107.1"
                     /translation="VFAIAPFIGAGLAVAIGGRAVTGWTLAAAGLFAAGVLLHLTERH
                     GHAHIHEPTEHDHVHRHDDGHHDHVHDPPFLGEHAHPHRHDQLVHTHDHAPDLHHAHD
                     H"
     gene            579..1238
                     /locus_tag="VF516_25435"
     CDS             579..1238
                     /locus_tag="VF516_25435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111108.1"
                     /translation="MGNPLARSNGNPPHSFLGDALKFAIGAAGGPFVDALEIGGVAAA
                     IVKQAIPALAAAVGNSVSKSGPSGPDSLATASFVTDYAHAVKEHHAIVTQRLESAIHN
                     VQDGKDIVGALGGEKIAGRLQLTSGQEGRVRLETQKETLDAWTVAMQKTGDKDEFGHD
                     DKTAAAMFYDQKSLFPVTSDEVKKDDAIVDRDYRKGLHKIWNIIHDQTPHQLGFSTKG
                     D"
     gene            complement(1286..>2275)
                     /locus_tag="VF516_25440"
     CDS             complement(1286..>2275)
                     /locus_tag="VF516_25440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111109.1"
                     /translation="ALVAAALAGALGLGLVADLVVRDRLTAEIQASFALIARQTNRAW
                     DGLTARFDTAANQVYSLAVMREAANRDNADFGLGDPRADAENLDRIHSALMSQDWTLV
                     QDFAGTQYSTTIAVADSKGRMLYSSAAPTAGRSDLTRLPWIRTALTQKDRTISLVRTD
                     DAALVASGLLGSSPPPGLGFLYTRTLVLGNDKNKEVASAFIEEVDAARLFGEIKLDEQ
                     TRLSIVAPGGTAIGNVPPALIRIAAITAGTVEAAADGATYQVLAQPIRGFDNQIVGDV
                     VMARKLDSVLLSLFPGARGVFALAMLGALAVAIASALWMRQIAVPGRAWRSAA"
BASE COUNT          375 a          815 c          794 g          291 t
ORIGIN      
        1 cggtgttcgc gatcgcgccg ttcatcggcg ccgggctcgc ggtcgcgatc ggcgggcgcg
       61 cggtcaccgg ctggacgctc gcggccgccg ggctgttcgc cgccggcgtc ctcctgcacc
      121 tcaccgagcg ccacggccac gcccacatcc acgagccgac cgagcacgac cacgtccacc
      181 gccacgacga cggccaccac gatcacgtcc acgatccgcc gttcctcggc gagcacgccc
      241 acccgcaccg ccacgaccag ctggtccaca cccacgacca cgcccccgat ctccaccacg
      301 cgcacgatca ctgacgcgat cctcgatccg cgcgaggtcc gggggggcat gaccaagaac
      361 tttggtctgt ctgaatacaa acctcgagac ggcgctgcgc gccatcgcgt acgcgagaaa
      421 tgtgagacgc gtcgatccgg agccgagagc gaggccgggg cgtaccgccc catatcaggg
      481 cgcgtgaacg cgcacgaaca ccaccccgcc gcagctcacc agggccctcg ttcgtccgcc
      541 gacgccgacg ccggggtcgg cacgacgcga tcatcagcat gggaaacccg ctggcgaggt
      601 cgaacggcaa tcccccgcac agcttcctgg gcgacgcact gaagttcgcg atcggggccg
      661 cgggcgggcc gttcgtcgac gcgctcgaga tcggcggggt cgccgcggcg atcgtcaagc
      721 aggcgatccc agcgctcgcg gccgcggtcg gcaacagcgt gagcaagagc ggaccgtccg
      781 gccccgactc gctcgccact gcttccttcg tcaccgatta cgcgcacgcc gtcaaggagc
      841 accatgcgat cgtgacccag aggctcgagt ccgcgatcca caacgtgcaa gacggcaagg
      901 acatcgtcgg ggcgctgggt ggcgagaaga tcgccgggcg cttgcagctc acctccggac
      961 aggaaggcag agtccggctg gagacccaga aagagacgct cgatgcctgg accgtcgcga
     1021 tgcagaagac cggcgacaag gacgagttcg gccacgacga caagacggcg gccgcgatgt
     1081 tctacgacca gaagagcctg ttcccggtga cctccgacga ggtcaagaag gacgacgcca
     1141 tcgtcgaccg cgactaccgc aaggggctcc acaagatctg gaacatcatc catgaccaga
     1201 cgccgcacca gctcgggttc tcgacgaagg gcgactagcc cggtcagcgc aggcggtggg
     1261 cgagcaggtc cggccagcgc cggggtcatg ccgcggatcg ccacgcgcgg ccgggcacgg
     1321 cgatctggcg catccacagc gccgaggcga tcgcgaccgc gagcgcgccg agcatggcga
     1381 gcgcgaacac cccccgcgcg ccggggaaca gcgacaagag cacgctgtcg agcttgcggg
     1441 ccatcacgac gtcgccgacg atctggttgt cgaacccgcg gatcggctgg gccagcacct
     1501 ggtaggtcgc gccgtcggcc gcggcctcga cggtgccggc ggtgatcgcg gcgatccgga
     1561 tcagcgcggg tggcacgttg ccgatcgcgg tgccgccggg cgcgacgatc gacagccggg
     1621 tctgctcgtc gagcttgatc tcgccgaaca gccgggcggc gtcgacctcc tcgatgaacg
     1681 cgctggcgac ctccttgttc ttgtcgttgc cgagcacgag cgtgcgggtg tagaggaagc
     1741 ccaagccggg cggcgggctg gacccgagca gcccggacgc gaccagcgcg gcgtcgtcgg
     1801 tgcggaccag gctgatcgtg cggtccttct gggtcagcgc ggtccggatc cacggcagcc
     1861 gggtcaggtc gctgcgcccg gcggtcggcg cggcgctcga gtacagcatc cggcccttgc
     1921 tgtcggcgac cgcgatcgtc gtcgagtact gcgtgcccgc gaagtcctgg accagcgtcc
     1981 agtcctgcga catcagcgcg ctgtggatcc ggtcgaggtt ctcggcgtcg gccctgggat
     2041 cgccgaggcc gaagtcggcg ttgtcgcggt tggccgcctc gcgcatcacc gcgagcgagt
     2101 agacctggtt ggcggcggtg tcgaaccgcg cggtcagccc gtcccacgcc cggttggtct
     2161 gccgggcgat cagcgcgaac gacgcctgga tctcggcggt cagccggtcg cggaccacga
     2221 ggtcggcgac cagaccgagc ccgagcgcgc cggcgagcgc ggcggcgacg agcgc
//
LOCUS       DASVOR010001334         6867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_275981,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001334 DASVOR010000000
VERSION     DASVOR010001334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275981"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1075
                     /locus_tag="VF516_25445"
     CDS             <1..1075
                     /locus_tag="VF516_25445"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEX8111110.1"
                     /translation="GKPVITVGAYLEEYYQLQFQDASNRITNLRGFDNRSRTFTLSNV
                     ALDVKGETGPITEHVVLQIGHTPSTYYLAEPALAGTASVNATGPELWKYLQAANITAK
                     APHDLVIEAGLFPSPIGPEVLPVKDNWNWSRSNLFFGLPFYHTGAMISHALGEGWTGK
                     LHLYNGWNSVTDNNGTPSVAISAAYASGDTTAQLMYFGGNERPDGAPEGKPWRNLFDA
                     ILQIPLTGDVTVMLHGDAGFEPNHYGTSGWAAGAAYAKLQLDPRLYAAVRGDAFYEKV
                     AANSAGTASAIFWPVQWVAEGTLTLAYQPVDNASLRLEYRHDQAAGNAYFGGDVITDP
                     VTHAFVPNRKSQDTVTLGVTAWF"
     gene            1191..1283
                     /gene="kdpF"
                     /locus_tag="VF516_25450"
     CDS             1191..1283
                     /gene="kdpF"
                     /locus_tag="VF516_25450"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEX8111111.1"
                     /translation="MTALFWLAGILSVGLLIYLFVALFQPEKLQ"
     gene            1280..3052
                     /gene="kdpA"
                     /locus_tag="VF516_25455"
     CDS             1280..3052
                     /gene="kdpA"
                     /locus_tag="VF516_25455"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF015753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEX8111112.1"
                     /translation="MTATGIGQIVLYAIVLVALGIPLGAYMARVYTGEARFAQRVLGP
                     LERLLYRLFGVKPDQEQTWKQYAVAAIVFNVLGIATVYLLQRLQAHLPENPNSLPSVD
                     PRVAFNTAVSFGTNTNWQAYAGEAAMSHLVQSAALGVQNFVSAATGMAVLIPLIRGFA
                     RKHAETIGNFWVDLTRSTVYILLPLSIVLAVALIHQGVPQTTDGKETAALLDAQPGTP
                     PADAKPGTVVPPVTEQPIAVGPVASQVAIKQLGTNGGGYYNVNSAHPLENPTPLSNFL
                     EDIAILLIAGATCFMFGRMVGSRKQGVALIVTMAILLVPFVALTLHQEQQPNPALTSL
                     SVDSHASALQPGGNMEGKEARFGISASALWATTTTAASNGSVNSMHDSYSPLGGLSPM
                     FLIALGEVSFGGVGSGLYGLLIFAIVAVFIAGLMVGRTPEFLGKKIEAYEMKMASWAI
                     LVPVAAVLLGAAFACLVDETYTKALANHGPHGFSEVLYAFVSQGNNNGSAFAGLTASG
                     TFFATAGGIVMFVARFWIVIPTLAIAGSLARKKLVPPSSGTLPTHGPLFVVLLVGVVL
                     LVGALSFVPALALGPIVEHLVLHP"
     gene            3070..5190
                     /gene="kdpB"
                     /locus_tag="VF516_25460"
     CDS             3070..5190
                     /gene="kdpB"
                     /locus_tag="VF516_25460"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEX8111113.1"
                     /translation="MSIHPRVTARPLFDGPIVRRAIRDAFGKLDPRRMIRNPVMFVVE
                     IGSAFTTLLFVHALVTGAGDASPGFIAAISVWLWFTVLFANFAEAMAEGRGKAQADAL
                     RKARKDVPAFVLAEPRRDAPKQEKPSSQLRPGDVVLVEAGQIIPGDGDILEGVASVNE
                     SAITGESAPVIRESGGDRSAVTGGTTVLSDWLILRITARPGESFLDRMISMVEGAKRK
                     KTPNEIALDILLAGLTIVFLLVTVTLYPFGVFAAREMAAAGGTAQVVTLTVLVALLVC
                     LIPTTIGGLLSAIGIAGMDRMIQANVIATSGRAVEAAGDVDVLLLDKTGTITHGNRQA
                     TAFFPAAGVSEAQLAEAAVMASLSDDTPEGKSIVALGVGKLGRPVTVPEGARPVPFTA
                     QTRMSGVDFNGRTIRKGASDAIARHVAITGDPHRPGGGLPADVRASVDNVAKRGATPL
                     VVADGGSVLGVVQLADVVKDKIRDRFGEMRRMGIKTVMITGDNPVTAAAIAAEAGVDD
                     FLAEATPENKLAMIRDYQDKGHLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGN
                     MVDLDSNPTKLLEVVTVGKQMLMTRGALTTFSIANDVSKYFAIIPAAFVGTYPQLEAL
                     NIMRLHSPTSAVLSAVIFNALIIIALIPLALRGLTVRAAPAAVTLRRNILIYGVGGLI
                     LPFPFIKLIDVILSGLGLAWVRSP"
     gene            5187..5810
                     /gene="kdpC"
                     /locus_tag="VF516_25465"
     CDS             5187..5810
                     /gene="kdpC"
                     /locus_tag="VF516_25465"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEX8111114.1"
                     /translation="MKETLRPAIVSLLAFTALCGFVYPLVMDGIAAVAFPHQATGSLI
                     HDKDGRVVGSELIGQPFDNPAYFWSRLTAEASYDASNSTGTNLGPTSFVDAHGHLGPN
                     TALARSARDRIAALRAADPGNTQPVPVDLVTSSGSGLDPHISPAAAYYQAGRVARLRG
                     QPEAAVRQLVDDHVEGRTLGVLGEPRVNVLLLNRALDDRFGPAKPHG"
     gene            5803..>6867
                     /locus_tag="VF516_25470"
     CDS             5803..>6867
                     /locus_tag="VF516_25470"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEX8111115.1"
                     /translation="MDDDRDDRPDPDALLENLKAEESRAVRARLKIFFGAAPGVGKTY
                     AMLQEARRARGSGDDGDDVVIGVVETHGRPETEELVTGLPVIPRRVIEHRGVRLTELD
                     LDAALARRPARILIDELAHTNAPGSKHVKRWQDVLELLDAGIDVHTTVNVQHIESLSD
                     VIQQITGVRVRETVPDVLFERADELELVDISPDELLDRLAEGKVYVPEQAQRAVQNFF
                     QKGNLLALRELALRRTAERVDAEVLAYRKQHGISSLWPTAERILVCVGPSPGSERLIR
                     ATKRIAEGLHASWGAAHVEVLGAPPLNDRDRERVEAHLRLAEALGGEVIRLAGQTVAG
                     VLLEHARQTNVTRIVAGKPTH"
BASE COUNT         1034 a         2435 c         2369 g         1029 t
ORIGIN      
        1 gggcaagccg gtgatcacgg tcggggccta cctggaggag tactaccagc tccagttcca
       61 ggatgcttcg aaccgcatca ccaacctgcg cgggttcgac aaccgcagcc gcacgttcac
      121 gctgtccaac gtcgcgctcg acgtcaaggg cgagaccggg ccgatcaccg agcacgtggt
      181 gctgcagatc ggccacacgc cgtcgaccta ctacctcgcc gagccggcgc tcgccgggac
      241 cgccagcgtg aacgccaccg gccccgagct atggaagtac ctccaggccg cgaacatcac
      301 cgcgaaggcg ccgcatgacc tcgtgatcga ggccggcttg ttcccgtctc cgatcggccc
      361 cgaggtcctg ccggtcaagg acaactggaa ctggtcgcga tcgaacctgt tcttcggact
      421 gccgttctat cacaccggcg cgatgatctc gcacgcgctc ggcgagggct ggaccggcaa
      481 gctgcacctc tacaacggct ggaactcggt caccgacaac aacggcacac cgagcgtggc
      541 gatctcggcg gcgtacgcga gcggcgacac caccgcgcag ctgatgtact tcggtggcaa
      601 cgagcgcccc gacggcgcgc cggaaggcaa gccgtggcgc aacctgttcg atgcgatcct
      661 gcagatcccg ctgaccggcg atgtgaccgt gatgctgcac ggggacgcgg ggttcgagcc
      721 caaccactac ggcacgagcg ggtgggccgc gggcgctgcc tacgccaagc tccagctcga
      781 ccccaggctg tacgcggccg tgcgcgggga cgcgttctac gagaaggtcg ctgcgaacag
      841 cgcaggcacc gcgagcgcga tcttctggcc ggtccagtgg gtcgccgagg gcacgctgac
      901 gctggcgtac cagccggtcg acaatgcctc gctgcgcctc gagtaccgcc acgaccaggc
      961 ggccggcaat gcctacttcg gcggcgacgt gatcaccgac ccggtgacgc acgcgttcgt
     1021 tcccaaccgc aagtcgcagg acaccgtgac gctcggagtc accgcatggt tctgatgagg
     1081 agtcctgatg ttcgatctac tctacctggt tctcatcctc gcgctgttcg ggatctcgat
     1141 cgtgatgatc cggttcttcg accggctgtg agccgagagg agatgaagcg atgactgccc
     1201 tgttctggct cgccggcatc ctgtccgtcg gcctgctcat ctatctgttt gtcgcgctgt
     1261 tccagccgga gaagctgcaa tgaccgccac cgggatcggc cagatcgttc tctatgcgat
     1321 cgtgctggtc gcgctcggca tcccgctcgg ggcgtacatg gcgcgggtct acaccggcga
     1381 ggcgcggttc gcccagcgcg tgctcggccc gctggagcgc ctgctgtacc ggctgttcgg
     1441 ggtcaagccc gaccaggagc agacctggaa gcagtacgcg gtcgccgcga tcgtgttcaa
     1501 cgtgcttggc atcgcaaccg tgtacctgct gcagcggctg caggcgcacc tgccggagaa
     1561 cccgaacagc ctgcccagcg tcgatccgcg ggtcgcgttc aacaccgcgg tcagcttcgg
     1621 gaccaacacg aactggcaag cctatgccgg cgaggctgcg atgagccacc tggtccagtc
     1681 ggccgcgctg ggcgtccaga acttcgtgtc ggcggcgacc ggcatggcgg tgctgatccc
     1741 gctgatccgc ggcttcgcgc gcaagcatgc cgagaccatc ggcaacttct gggtcgacct
     1801 gacgcgctcg acggtctaca tcctcctgcc gctgtcgatc gtgctggcgg tggcgctgat
     1861 ccaccagggc gtaccgcaga ccaccgacgg caaggagacc gcggcgctgc tcgacgccca
     1921 gcccggcacg ccgccggccg atgccaagcc ggggaccgtg gtgccgccgg tgaccgagca
     1981 gccgatcgcg gtcggcccgg tcgcgtcgca ggtcgcgatc aagcagctcg gcaccaacgg
     2041 cggtggctac tacaacgtga actcggcgca cccgctcgag aacccgacgc cgctgtcgaa
     2101 tttcctggag gacatcgcga tcctgctgat cgccggcgcg acctgcttca tgttcggccg
     2161 gatggtcggc tcgcgcaagc agggcgtcgc gctgatcgtg acgatggcga tcctgctggt
     2221 gccgttcgtg gcgctgacgc tgcaccagga gcagcagccc aacccggcgc tgacctcgct
     2281 gtcggtcgat tcgcacgcgt cggcgctgca gcccggcggc aacatggagg gcaaggaggc
     2341 ccggttcggg atctcggcgt ccgcgctgtg ggcgaccacg accaccgcgg cgtcgaacgg
     2401 ctcggtcaac tcgatgcacg acagctactc gccgctcggc gggctgtcgc cgatgttcct
     2461 gatcgcgctc ggcgaggtgt cgttcggcgg cgtcggctcg gggctgtacg gcctcttgat
     2521 cttcgcgatc gtcgcggtgt tcatcgccgg cctgatggtg ggccggacgc cggagttcct
     2581 cggcaagaag atcgaggcct acgagatgaa gatggcgtcg tgggcgatcc tggtcccggt
     2641 cgccgccgtg ctgctcgggg ccgcgttcgc gtgcctggtc gacgagacct ataccaaggc
     2701 gctcgccaac cacgggccgc acgggttctc cgaggtgctg tatgcgttcg tgtcgcaggg
     2761 caacaacaac ggctcggcgt tcgccgggct gaccgcgagc ggcacgttct tcgccaccgc
     2821 cggcggcatc gtcatgttcg tcgcgcggtt ctggatcgtg atcccgacgc tggcgatcgc
     2881 cggctcgctg gccaggaaga agctcgtgcc gccgtcgtcg ggcacgctgc cgacgcacgg
     2941 cccgctgttc gtcgtgctgc tggtcggcgt cgtgctgctg gtcggcgccc tgtcgttcgt
     3001 ccccgcgctg gcgctcggcc cgatcgtcga gcacctggtg ctccatccgt gagccggagc
     3061 tgaaccgcca tgtcgatcca tcctcgtgtc accgcccgcc ctctgttcga cgggccgatc
     3121 gtccgccgcg cgatccggga cgcgttcggc aagctcgatc cgcgccgcat gatccgcaac
     3181 ccggtgatgt tcgtcgtcga gatcgggagc gcgttcacca ccctcctgtt cgtccacgcg
     3241 ctggtcaccg gcgccggcga tgcctcgccc ggcttcatcg ccgcgatcag cgtgtggctg
     3301 tggttcaccg tgctgttcgc caacttcgcc gaggcgatgg ccgagggccg cggcaaggcg
     3361 caggccgacg ctctgcgcaa ggcgcgcaag gacgtgccgg cgttcgtgct ggccgagccg
     3421 cggcgcgacg cgccgaagca ggagaagccg tcgtcgcagc tccggcccgg cgacgtcgtg
     3481 ctggtcgagg ccggccagat catcccgggc gacggcgaca tcctcgaggg cgtcgcgtcg
     3541 gtcaacgaga gcgcgatcac cggcgagagc gccccggtga tccgcgagag cggcggcgac
     3601 cgcagcgcgg tgaccggcgg cacgaccgtg ctgtcggact ggctgatcct ccggatcacc
     3661 gccaggccgg gcgagagctt cctcgaccgg atgatctcga tggtcgaggg cgccaagcgc
     3721 aagaagaccc cgaacgagat cgcgctcgac atcctgctcg ccgggctgac catcgtgttc
     3781 ctgctggtca ccgtcacgct gtacccgttc ggcgtgttcg cggcccgcga gatggcggcc
     3841 gccggcggca ccgcgcaggt cgtgaccctg accgtgctgg tcgcgctgct ggtctgcctg
     3901 atcccgacga cgatcggcgg gctgctgtcg gcgatcggga tcgccggcat ggaccggatg
     3961 atccaggcca acgtgatcgc gaccagcggc cgcgcggtcg aggccgccgg cgacgtggat
     4021 gtcctgctgc tcgacaagac cggcacgatc acgcacggca accgccaggc caccgcgttc
     4081 ttcccggcgg ccggggtcag cgaggcgcag ctcgccgagg ccgcggtgat ggcgtccttg
     4141 tccgacgaca cgccggaggg caagagcatc gtcgcgctcg gggtcggcaa gctcggccgc
     4201 ccggtcaccg tgcccgaggg cgcgcggccg gtgccgttca ccgcgcagac ccggatgagc
     4261 ggcgtcgact tcaacggtcg cacgatccga aaaggggcga gcgacgcgat cgcccgccac
     4321 gtcgcgatca ccggcgatcc gcaccgtccg ggcggcggac tgccggccga cgtgcgcgcc
     4381 tcggtcgaca acgtcgccaa gcgcggcgcg acgccgctgg tggtcgccga cggcggcagc
     4441 gtgctcggcg tggtccagct cgccgacgtg gtcaaggaca agatccgcga ccggttcggc
     4501 gagatgcgcc ggatggggat caagaccgtg atgatcaccg gcgacaaccc ggtgaccgcc
     4561 gcggcgatcg ccgccgaggc cggggtcgac gacttcctcg ccgaggccac gccggagaac
     4621 aagctggcga tgatccgcga ctaccaggac aagggccacc tggtcgcgat gaccggcgac
     4681 ggcaccaacg acgcgccggc gctcgcccag gccgacgtcg cggtcgcgat gaacagcggc
     4741 acccaggccg ccaaggaggc cggcaacatg gtcgacctcg actccaaccc gaccaagctg
     4801 ctcgaggtcg tcaccgtcgg caagcagatg ctgatgacgc gcggcgcgct gaccacgttc
     4861 tcgatcgcca acgacgtgtc gaagtacttc gcgatcatcc cggccgcgtt cgtcgggacc
     4921 tacccgcagc tcgaggcgct caacatcatg cgcctgcact cgccgaccag cgccgtgctg
     4981 tcggcggtga tcttcaacgc gctgatcatc atcgcgctga tcccgctcgc gctgcgcggc
     5041 ctgaccgtgc gcgcagcgcc ggccgccgtc acgctgcgcc gcaatatcct gatctacggc
     5101 gtcggcgggc tgatcctgcc gttcccgttc atcaagctga tcgacgtgat cctgtccggc
     5161 ctgggcctgg cctgggtgag gtcgccatga aggaaaccct ccgccccgcc atcgtctcgc
     5221 tgctcgcgtt caccgcgctg tgcggcttcg tctatccgct ggtgatggac gggatcgccg
     5281 ccgtggcgtt cccgcaccag gccaccggat cgctgatcca cgacaaggac ggccgcgtcg
     5341 tcggctccga gctgatcggc cagccgttcg acaacccggc gtacttctgg agccggctga
     5401 ccgccgaggc cagctacgac gcgtcgaact cgaccgggac caacctcggc ccgacgagct
     5461 tcgtcgacgc gcacggccac ctcggcccga acacggcgct ggccaggagc gcccgggacc
     5521 ggatcgccgc cctgcgcgcc gccgatccgg ggaacaccca gccggtgccg gtcgacctgg
     5581 tcacgtcgtc gggctcgggg ctcgatccgc acatctcgcc ggcggcggcg tactaccagg
     5641 ccggccgggt cgcccggctg cgcggccagc ccgaggcggc ggtgcgtcag ctggtcgatg
     5701 atcacgtcga gggccggacg ctcggcgtgc tgggcgagcc ccgggtgaat gtcctactct
     5761 tgaaccgggc cctcgatgac aggttcgggc ccgcgaagcc gcatggatga cgaccgcgac
     5821 gaccgccccg atcccgatgc cttgctggag aacctcaagg ccgaggagag ccgtgcggtc
     5881 cgcgcgcggc tgaagatctt cttcggggcg gcgcccgggg tcggcaagac ctacgcgatg
     5941 ctgcaggagg cccggcgggc gcggggcagc ggcgacgatg gtgacgatgt cgtgatcggc
     6001 gtcgtcgaga cccacggccg acccgagacc gaggagctgg tcaccggcct gccggtgatc
     6061 ccgcgccggg tgatcgagca ccgcggcgtc cggctgaccg agctcgatct cgacgccgcg
     6121 ctggcgcgcc ggccggcgcg gatcctgatc gacgagctcg cccacaccaa cgcgccgggc
     6181 agcaagcacg tcaagcgctg gcaggacgtg ctcgagctgc tcgacgccgg gatcgatgtc
     6241 cacaccacgg tcaacgtcca gcacatcgag agcctgtccg acgtgatcca gcagatcacc
     6301 ggggtccggg tgcgcgagac cgtgcccgac gtgctgttcg agcgcgccga cgagctcgag
     6361 ctggtcgaca tctcgcccga cgagctgctc gaccggctcg ccgagggcaa ggtctacgtc
     6421 cccgagcagg cccagcgcgc ggtccagaac ttcttccaga agggcaacct gctcgcgctg
     6481 cgcgagctcg cgctgcgccg cacggccgag cgggtcgacg ccgaggtcct cgcgtacaga
     6541 aagcagcacg ggatctcgtc gctgtggccg accgccgagc ggatcctggt gtgcgtcggc
     6601 ccgagcccgg gctcggagcg gctgatccgc gcgaccaagc ggatcgccga gggcctgcac
     6661 gccagctggg gcgccgcgca cgtcgaggtg ctcggcgcgc cgccgctcaa tgacagggac
     6721 cgcgagcggg tcgaggccca cctgcggctc gccgaggcgc tcggcggcga ggtgatccgg
     6781 ctcgccgggc agaccgtggc gggcgtgctg ctcgagcacg cgcggcagac caacgtcacc
     6841 cggatcgtcg ccggcaagcc gacccac
//
LOCUS       DASVOR010001335         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_72014,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001335 DASVOR010000000
VERSION     DASVOR010001335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72014"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="VF516_25475"
     CDS             complement(<1..590)
                     /locus_tag="VF516_25475"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8111116.1"
                     /translation="MTAAPAAIELVGVAKSYGQYRAVDKVDLVVPHGGVTALIGPNGA
                     GKSTLFGIVAGDLAADTGRVLMAGQDVSRVSASGRARLGLGRTFQTARVFERLSALEN
                     NVLAVRAADRAALRVTDQPRGNRRVVERARAGLELVGLGRQADRTAALLAQGERKRLE
                     IALALTARPRILLLDEPTAGVGREDAQSFIRLIGSLR"
     gene            complement(587..1618)
                     /locus_tag="VF516_25480"
     CDS             complement(587..1618)
                     /locus_tag="VF516_25480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8111117.1"
                     /translation="MIIAANAIAVSALAGADVYTWSLILVYGLFASSTNILFGWVGRA
                     SFGQAAFFGTGAYMVAFLREQDVPPLLALLVGLGAGVVLGIPLLILLRRSSGITFAMI
                     TLIVAQIIYQLVFTNRRLGGETGLSGITVTSVLGQDLTSYQAFSWYVASVVAIAMVVL
                     WRLSESSFGMSLRAVRDDETKAAIVGIPVRRAQAIAFLVAGGLAGLAGAMLAQVQSTV
                     SADLLFWTVSGNAVIMCLLGGRYRFWGPFLGAAVFIWVQNAFLQTGALSDLLIGAVFL
                     VIVLVVPDGILGSVSPDRVGALVRRARTARGIGSSVAAGPVKSTVDPDAAGAPQPVRT
                     GSDRGEGAR"
     gene            complement(1704..>2586)
                     /locus_tag="VF516_25485"
     CDS             complement(1704..>2586)
                     /locus_tag="VF516_25485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8111118.1"
                     /translation="ALLSGLSTGVPLFLIASGLTLIFGVMGILNFAHGGFFMLGAFVA
                     VSAAGWFGGGSGGWTLFGAVVVAAVAIAAVAVVCDLVVFRRLQKREEIIGLLGTFALL
                     LLLVGVAEQVWGVTPRSLTMPTVLSKQIELGPASITTYDVMLIVIGLLVAVGLWFLLR
                     RTDFGRKARAVAFDRSMAAALGIKASLVGTGVVAIGGLLAGLAGGLVAPSVSVDAGLS
                     LSFIVPCFAIVLIGGAGSITGALVAAIILGVAQSLVVNYAPSFFGYATYVVIGALLLL
                     RAWIRNLRPAEEREQVA"
BASE COUNT          441 a          962 c          908 g          275 t
ORIGIN      
        1 cgcagcgagc cgatcagccg gatgaacgac tgggcgtcct cccggccgac ccctgcggtc
       61 ggctcgtcca gcagcaggat ccgcggccgg gccgtcagcg ccagcgcgat ctccagccgc
      121 ttgcgctcgc cctgcgccag cagcgccgcg gtccggtccg cctgccggcc caggccgacc
      181 agctccaggc cggcccgcgc gcgctcgacc acccgccggt tgccgcgcgg ctggtcggtg
      241 acccgcagcg cggcccggtc cgcggcccgt acggcgagca cgttgttctc cagggcggac
      301 agccgctcga acacccgcgc ggtctggaag gtgcggccga gcccgagccg ggcgcgaccg
      361 gacgcgctga cccggctgac gtcctgcccg gccatcagca cccggccggt gtccgcggcc
      421 aggtcgccgg ccacgatgcc gaacagcgtc gacttgccgg cgccgttggg tccgatcagc
      481 gccgtcaccc cgccgtgcgg gacgacgagg tcgaccttgt cgaccgcccg gtactgcccg
      541 tacgacttgg cgacgccgac cagttcgatg gcggccggtg ccgcggtcat cgggccccct
      601 ccccgcgatc ggaaccggtg cgcaccggct gcggagcgcc agccgcgtct gggtccacag
      661 tggacttcac cggcccggcc gcgacggagc tgccgatgcc gcgggcggtc ctggcccggc
      721 ggaccagcgc accgacccgg tccggggaga ccgagccgag gatgccgtcc ggcacgacca
      781 gcacgatgac caggaacacc gcgccgatca gcaggtcgga cagcgctccg gtctgcagga
      841 aggcgttctg cacccagatg aacacggccg cgccgaggaa cgggccccag aaccggtacc
      901 ggccgccgag caggcacatg atcaccgcgt tgccggagac cgtccagaac agcaggtctg
      961 ccgagaccgt gctctgcacc tgcgccagca tcgcgccggc cagcccggcc agcccgccgg
     1021 cgacgaggaa cgcgatggcc tgcgcccggc gtaccgggat cccgacgatc gcggccttgg
     1081 tctcgtcgtc gcgtactgcc cgcagcgaca tcccgaacga ggactcgctc agccgccaca
     1141 gcacgaccat cgcgatcgcg acgacggagg cgacgtacca gctgaacgcc tggtagctgg
     1201 tgaggtcctg gccgagcacc gaggtcaccg tgatgccgga cagcccggtc tcgccgccga
     1261 gccggcggtt ggtgaacacc agctggtaga tgatctgcgc gacgatcagc gtgatcatcg
     1321 cgaaggtgat gccggagctg cgccggagca ggatcagcag cgggatgccc agcacgacgc
     1381 cggcgccgag cccgaccagc agcgccagca gcggcggcac gtcctgctcg cgcaggaacg
     1441 cgaccatgta cgcgccggtg ccgaagaatg cggcctgccc gaacgaggcc cgcccgaccc
     1501 agccgaacag gatgttcgtc gagctggcga acaggccgta gacgaggatc agcgaccagg
     1561 tgtagacgtc ggcaccggcc agcgcggaga ccgcgatcgc gttggcggcg atgatcacga
     1621 ctgccggcag cggccaggac caccgggacc agccgcggat ccgcgagggg ccggggagac
     1681 cggttggcgc cgggactggt gcgtcaggca acctgctcac gctcctccgc cggccgcagg
     1741 ttcctgatcc aggcccggag caggagcagg gcgccgatca ccacgtaggt ggcgtagccg
     1801 aagaagctcg gcgcgtagtt caccaccagg ctctgcgcga cgccgaggat gatcgcggcc
     1861 accagcgcgc cggtgatcga gccggctccg ccgatcagta cgatcgcgaa gcacggcacg
     1921 atgaacgaga gcgagagccc ggcgtccacc gagacgctcg gcgcgaccag gccgccggcc
     1981 agcccggcca gcagaccgcc gatcgcgacg acgccggtgc cgaccagcga ggccttgatg
     2041 cccagcgcgg cggccatgga gcggtcgaac gcgaccgccc gcgccttgcg cccgaagtcc
     2101 gtcctgcgca gtaggaacca cagccccacg gcgacgagca ggccgatgac gatgagcatc
     2161 acgtcgtacg tggtgatcga cgccggcccg agctcgatct gcttcgacag cacggtgggc
     2221 atggtcagcg accgcggggt gacaccccag acctgctcgg ccacgccgac cagcagcagt
     2281 agcagggcga acgtgccgag caggccgatg atctcctcgc gtttctgcag ccggcggaac
     2341 accacgaggt cgcagacgac ggcgaccgcg gcgatcgcca ccgcggccac cacgaccgcg
     2401 ccgaacagcg tccatccgcc ggaaccgccg ccgaaccagc cggcggcgga gaccgcgacg
     2461 aacgcgccca gcatgaagaa cccgccgtgg gcgaagttga ggatgcccat cacgccgaag
     2521 atcagggtca gcccgctggc gatgaggaac agcggcaccc cggtcgagag cccggacagc
     2581 agggcg
//
LOCUS       DASVOR010001336         2465 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_275993,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001336 DASVOR010000000
VERSION     DASVOR010001336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275993"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VF516_25490"
     CDS             complement(<1..827)
                     /locus_tag="VF516_25490"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8111119.1"
                     /translation="MIASRAPAVRDAARDHRQAIALLALCAVLWSTAGVLIKLVHWNA
                     GAIWSTRSAIAAVALWLWRRPSFARISAAEWRGACSLAATTGLFILANQRTTAANAIL
                     IQYSAPIWVALLGAWLLGERATRLDWLTIVTVIGGIVLFFFEQLSFDHVVGNLVALGA
                     GVAFAVNVVSFRQVAVTPGATGPSDPLRPMLLGNLIGAVLGAPFLLASPPPAATGWGA
                     LIALGLFQQAAAYLCYAWAIRHATAIEATLVAVIEPILSPVWVVIAFGERPGPWAVLG
                     "
     gene            complement(907..2109)
                     /locus_tag="VF516_25495"
     CDS             complement(907..2109)
                     /locus_tag="VF516_25495"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8111120.1"
                     /translation="MSLVTTALGIAAFASLALITGCGNKKEPPAAEIKIGQSLPYSGP
                     ASAYAMLGKGEAAYFQMINDKGGINGRKIKFISLDDGYSPPKAVENVKKLVENENVLL
                     IFNNTGTAHNAAVQKYLNDKKVPQLFVATGADRWADPAHFPWTMGFQPSYRGEARIYA
                     RYLKKEKPDAKLCVLFQNDDFGKEYLAGLKDVLGDQYDKTVIKTQSYEVTDPSVDSQI
                     VTLQGAGCDALLTAATAKFAAQTIRKVGDLGWKPLHIMSGVSISRAAVLAPAGLDKST
                     GLISAGYIKDINDPALANDPGLNEYRAFAKQYLTGLDLTDGALEYAYGVAQTLVQVLT
                     QCGNDLSRENIMKQAANLKNLQLGVFGPGIAINTSPTDFRPFQQLQLARFNGNNFEPF
                     GEVIDTEQ"
BASE COUNT          367 a          810 c          902 g          386 t
ORIGIN      
        1 ccgagcacgg cccacgggcc ggggcgctcg ccgaacgcga tcacgaccca gaccggcgac
       61 aggatcggct cgatcaccgc gaccagcgtg gcctcgatcg cggtggcgtg ccggatcgcc
      121 cacgcgtagc acaggtaggc ggccgcctgc tggaacaggc cgagcgcgat cagtgcgccc
      181 cagccggtgg cggcgggcgg cggcgacgcg agcaggaacg gcgcgccgag caccgcgccg
      241 atcaggttgc cgagcagcat cgggcgcagc gggtcggacg ggccggtggc gcccggggtg
      301 accgcgacct ggcggaacga caccacgttg accgcgaacg cgacgccggc gccgagcgcg
      361 accaggttgc cgacgacgtg gtcgaagctg agctgctcga agaagaacag cacgatgccg
      421 ccgatcacgg tgacgatggt gagccagtcg agccgggtcg cgcgctcgcc gagcagccac
      481 gcgccgagca gcgcgaccca gatcggcgcc gagtactgga tcaggatcgc gttggcggcg
      541 gtggtccgct ggttggcgag gatgaacagc ccggtggtcg cggccagcga gcacgcgccg
      601 cgccactcgg ccgccgagat ccgggcgaac gacggccggc gccacagcca cagcgcgacc
      661 gcggcgatcg cgctgcgggt cgaccagatc gcgccggcgt tccagtggac gagcttgatc
      721 agcacgccgg cggtgctcca cagcacggcg cacagcgcga gcagggcgat cgcctggcgg
      781 tgatcgcgcg cggcgtcgcg gacggcggga gcgcgcgagg cgatcacggc gcgcggaaca
      841 taccgcagcg cggcgccggc gcggcgcgca cgcggcgctg atcgcccggc gcgcgggcca
      901 gctgtgtcat tgctcggtgt cgatcacctc gccgaacggc tcgaagttgt tgccgttgaa
      961 ccgggcgagc tggagctgct ggaacgggcg gaagtcggtc gggctggtgt tgatcgcgat
     1021 ccccgggccg aagaccccga gctgcaggtt cttgaggttc gcggcctgct tcatgatgtt
     1081 ctcgcgcgac aggtcgttgc cgcactgggt gagcacctgg accagggtct gggccacgcc
     1141 gtaggcgtat tccagcgcgc cgtcggtcag gtcgaggccg gtcaggtact gcttggcgaa
     1201 cgcgcggtac tcgttgaggc cgggatcgtt ggccagggca gggtcgttga tgtccttgat
     1261 gtagccggcg ctgatcaggc cggtgctctt gtcgaggccg gccggcgcga gcaccgcggc
     1321 gcgcgagatc gagacgccgg acatgatgtg gagcggcttc cacccgaggt cgccgacctt
     1381 gcggatggtc tgcgccgcga acttcgcggt cgccgcggtc agcagggcgt cacagcccgc
     1441 gccctgcagg gtgacgatct gcgagtcgac gctcggatcg gtgacctcgt acgactgggt
     1501 cttgatcacg gtcttgtcgt actggtcgcc gagcacgtcc ttgaggccgg ccaggtactc
     1561 cttgccgaag tcgtcgttct ggaacagcac gcacagcttg gcgtccggct tctccttctt
     1621 caggtagcgc gcgtagatcc gggcctcgcc gcggtagctg ggctggaagc ccatcgtcca
     1681 cgggaagtgc gccggatcgg cccagcggtc ggcgccggtc gcgacgaaca gctgcgggac
     1741 cttcttgtcg ttgaggtact tctggaccgc ggcgttgtgc gcggtgccgg tgttgttgaa
     1801 gatcagcaag acgttctcgt tctcgaccag cttcttgacg ttctcgaccg ccttgggcgg
     1861 cgagtagccg tcgtcgaggc tgatgaactt gatcttgcgg ccgttgatcc cgcccttgtc
     1921 gttgatcatc tggaagtacg ccgcctcgcc cttgccgagc atcgcgtagg ccgatgccgg
     1981 tccgctgtac gggagcgact ggccgatctt gatctcggcg gcgggcggtt ccttcttgtt
     2041 accgcaaccg gtgatcagcg cgaggctggc gaacgcggcg atcccgagcg cggtcgtgac
     2101 gagcgacatc ggtctggaca tgaatcctcc cgtttcgagc ggtgtgatga gagcaagcgg
     2161 cccgacacta ccgcattccg cctacgggat cgcccggccc gaggcaagat cagctctcgg
     2221 tgaacgtgta ctcgtgctgc gcgtcgcgga tcaccagcgt gcgcttgccg ccggccgagc
     2281 cgaccacggc ctccaggccg atcacgccgg gagcggtcat cacgaaggac accgagccgt
     2341 cggcgttctt gcgcgacccg accggggact accattcccc gacgtcgaag taggtcatgc
     2401 cccggtcgcg cacgacggcg atctcgccga gcgcgtcgtt gtggtagcgc gcggccagct
     2461 tgccg
//
LOCUS       DASVOR010001337         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43211,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001337 DASVOR010000000
VERSION     DASVOR010001337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43211"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..524)
                     /locus_tag="VF516_25500"
     CDS             complement(<1..524)
                     /locus_tag="VF516_25500"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX8111121.1"
                     /translation="MTPGPEPDGPDRVDCAARAERRTALRELAWLFARLGLTAFGGPA
                     GHIAMMEDEVVRRRGWMTRERLLDLIGAANVIPGPTSTELAIHIGHERGGWPGLVVAG
                     ACFILPAVAIVLAIAWAYVRFGALPAVGGVLYGIKPVIIAVVVQALWGLGRSAVKSAW
                     LGAVGLVALAASLAG"
     gene            complement(521..1261)
                     /locus_tag="VF516_25505"
     CDS             complement(521..1261)
                     /locus_tag="VF516_25505"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8111122.1"
                     /translation="MRNDRRTFLTSLAVGLGASSTGIALVGCSRSASNPGTGPSSASG
                     AASGDKDDDEVTPAEDLMREHGVLRRVMYLYDDAAERLTGHRDVPVDALAACAGIVRR
                     VIEDYHEKLEEDFLFPRFEKAGKLADLTAVLRRQHVAGRTLTEQITQLTRASLADADR
                     ARLAAALRSFNHMYRPHAAREDTVLFPELRGLLGKHAYEELGDQFEDKEKQMLGDHGF
                     EHAVEQVAKLEQAFGLDDLAKLTPTPAP"
     gene            1306..1506
                     /locus_tag="VF516_25510"
     CDS             1306..1506
                     /locus_tag="VF516_25510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111123.1"
                     /translation="MLSAAGREIRSGIWKIHILHHAAAHDVWGMWLLDELAEHGQRLS
                     PLYREVVLGDEPRHHSPKHPRR"
     gene            1509..1982
                     /locus_tag="VF516_25515"
     CDS             1509..1982
                     /locus_tag="VF516_25515"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8111124.1"
                     /translation="MFLNRQSPTERAIADQAPPTLVLLRIAMAFVFVTEGIQKFLYPE
                     LLGVGRFTKIGIPYPEFSAPLVGGVEVVGGALVLLGLYTRLAAFPLVIDMIVAITATK
                     VPILLGYGFWGFASPSGKTGFWAAAHEARLDIMMLLSCATLLVIGPGKLSFDQRR"
     gene            2081..>2309
                     /locus_tag="VF516_25520"
     CDS             2081..>2309
                     /locus_tag="VF516_25520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111125.1"
                     /translation="MIVIYGTRFYGEVDSHGGQRQLTRFFHIYYVPLVPVGTLWVTRD
                     AERGYNGHAVRMSGRSVLAGYARVWGPVAAVG"
BASE COUNT          319 a          844 c          790 g          356 t
ORIGIN      
        1 cccgccaggc tggcggccag cgcgaccagc ccgaccgcgc ccagccaggc ggacttgacc
       61 gccgaccgcc ccagccccca cagcgcctgg acgacgaccg cgatgatcac cggcttgatg
      121 ccatacagca cgccgccgac cgccggcagc gcgccgaacc gcacgtaggc ccatgcgatc
      181 gcgagcacga tcgcgaccgc gggcaggatg aagcacgccc cggcgacgac gaggccgggc
      241 cagccgccgc gctcgtggcc gatgtggatc gcgagctccg tcgaggtcgg gccggggatc
      301 acattggccg cgccgatcag gtcgagcagc cgctcccggg tcatccagcc ccggcgccgc
      361 acgacctcgt cctccatcat cgcgatgtgc cccgccgggc cgccgaacgc ggtgagcccg
      421 agccgggcaa acagccacgc cagctcgcgg agcgcggtcc gccgttccgc gcgcgcggcg
      481 cagtcgacgc ggtccggccc gtccggctcc ggcccggggg tcacggcgcc ggtgtcgggg
      541 tcagcttggc gaggtcatcg agcccgaacg cctgctccag cttggcgacc tgctcgaccg
      601 cgtgctcgaa cccgtggtcg ccgagcatct gcttctcctt gtcctcgaac tgatcgccga
      661 gctcctcgta cgcatgcttg ccgagcaggc cgcgcagctc ggggaacagc acggtgtcct
      721 cccgcgccgc atgcggccgg tacatgtggt tgaagctgcg cagcgccgcc gcgagccggg
      781 cgcgatcggc gtcggccagg gacgcccggg tcagctgcgt gatctgctcg gtgagcgtcc
      841 ggccggccac gtgctggcgc cgcaggaccg cggtgaggtc ggcgagcttg ccggccttct
      901 cgaaccgcgg gaacagaaag tcctcctcga gcttctcgtg gtagtcctcg atcacacgcc
      961 ggacgatacc ggcgcacgcc gccagcgcgt cgaccggcac gtcgcggtgc ccggtcaggc
     1021 gctcggccgc gtcgtcatag aggtacatca cgcgccgcag caccccgtgc tcgcgcatca
     1081 ggtcctcggc cggcgtgacc tcgtcgtcgt ccttgtcgcc cgacgccgct ccggatgcgg
     1141 agctcgggcc ggtccccggg ttcgacgccg accgggagca gccgaccagc gcgatgccgg
     1201 tgctggacgc tccgagacct acagccagcg atgtcaggaa cgtgcgtcga tcgttgcgca
     1261 tcggagttga tcctcgttta cggtgttcgt ttatgattac catggatgct atccgccgcc
     1321 ggccgtgaga tccggtcagg gatctggaag atccacattc ttcatcacgc cgcggcgcac
     1381 gatgtctggg ggatgtggct gctcgacgag ctcgccgagc acggtcagcg gttgagcccc
     1441 ctctatcgcg aggtggtgct cggcgatgaa ccccggcacc actccccaaa acacccccgg
     1501 aggtagtcat gtttctgaac cgccagagcc cgaccgagcg cgcgatcgcg gaccaggcgc
     1561 cgcccaccct ggtgctgctc cgcatcgcga tggcattcgt gttcgtcacc gagggtatcc
     1621 agaaattcct ctatccggag ctgctcggcg tcggccggtt caccaagatc gggattccct
     1681 atcccgagtt ctccgcgccg ctggtcggcg gcgtggaggt cgtcggcggc gcgctcgtgc
     1741 tgctcggcct gtacacccgg ctggccgcgt tcccgctggt gatcgacatg atcgtcgcga
     1801 tcacggcgac caaggtgccg atcctgctcg gctacgggtt ctggggcttc gccagcccca
     1861 gcggcaagac cgggttctgg gccgcggccc acgaggcgcg cctcgacatc atgatgctgc
     1921 tcagctgcgc aacgctgctc gtgatcgggc ccggcaagct gtcgttcgat cagcgccgct
     1981 gacgccgtcg tgccgcgggt ttgccgtgcg ccactctcgc gatccccgcg ccggcgggcc
     2041 cgtgttacgc tgcggccctg accgggaggc ggagcgcagc atgatcgtca tttacgggac
     2101 gcggttctac ggcgaggtcg acagccacgg cggccagcgc cagctcacca ggttcttcca
     2161 catctactac gtgccgctgg tgccggtggg caccctgtgg gtgacccgcg acgccgagcg
     2221 cgggtacaac ggccacgcgg tgcggatgag cggccgcagc gtgctcgccg gctacgcccg
     2281 ggtgtggggc ccggtcgccg cggtcggcg
//
LOCUS       DASVOR010001338         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_481469,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001338 DASVOR010000000
VERSION     DASVOR010001338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481469"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..895
                     /locus_tag="VF516_25525"
     CDS             <1..895
                     /locus_tag="VF516_25525"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8111126.1"
                     /translation="DGQLGSWGFPEGGMGGVTGALRAAAEAFGAQIRTGAEVAKIRVT
                     GGHAAGVVLAGGDELDADTIIATAHPKTTFLRHLERGELPDDFVTAIERWKTRSGVVK
                     INLALDRLPDFTCKPGFDPEVHGGTIVLAESLDDLEHAFQDAVSGAPARLPFADVCIP
                     SVFDPTLAPSGKHVMSMFTQWVPHGFAAAPHTAELEAYADRLIARLDELAPGFTSSIL
                     HRQIIGPHAMETTYGLIGGNIFHGELSANQLFHMRPAPGYADFTTPIRGLYQASSATH
                     GGGGVTGIPALQAVRRVLRDS"
     gene            complement(903..1463)
                     /locus_tag="VF516_25530"
     CDS             complement(903..1463)
                     /locus_tag="VF516_25530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111127.1"
                     /translation="MLIGELEVALDVGQKLDSLGVTWLVGGSVASSILGEPRATADVD
                     LVADLRGRHVSALFAALVDTYYIDEDAAKAAVRERGAFNVIQLASMTKVDIYCSADEP
                     LAREELQRRIFVDIDGQRIPCASAEDIILQKLLWFVEGGGVSDRQWRDIRGVIKVQGE
                     VLDRAYLERHAASHGVLDLLHRALNE"
     gene            complement(1485..1757)
                     /locus_tag="VF516_25535"
     CDS             complement(1485..1757)
                     /locus_tag="VF516_25535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111128.1"
                     /translation="MMDGTAKGASDTSPEAENMMFRLWRQASPAQKLRKVFGVGKMVN
                     ELVRAELRKRYPDATPREIELRLAARNLDRETMIRAFHWDPELQGR"
     gene            complement(1883..>2106)
                     /locus_tag="VF516_25540"
     CDS             complement(1883..>2106)
                     /locus_tag="VF516_25540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111129.1"
                     /translation="RLELGEEPVYHALIRGAPDLDRELGEPLARAASDALSALAHRLD
                     HWAAVLDVHAFDVEVGVGRALELAEARLA"
BASE COUNT          316 a          783 c          697 g          310 t
ORIGIN      
        1 cgatggccag ctcggctcgt ggggcttccc cgagggcggc atgggcggcg tgaccggcgc
       61 gctgcgcgcc gccgccgagg cgttcggcgc gcagatccgc accggggccg aggtcgcgaa
      121 gatccgcgtg acgggcggtc acgccgccgg cgtcgtgctc gccggcggcg acgagctcga
      181 cgccgacacg atcatcgcca ccgcccaccc caagaccacg ttcctccgcc acctcgagcg
      241 cggcgagctg ccggacgact tcgtcaccgc gatcgagcgc tggaagaccc gcagcggcgt
      301 ggtcaagatc aacctcgcgc tcgaccggct gcccgacttc acctgcaagc ccgggttcga
      361 tcccgaggtc cacggcggca cgatcgtgct cgccgagtcg ctcgacgacc tcgagcacgc
      421 gttccaggac gcggtgtcgg gcgccccggc gcggctgccg ttcgccgacg tctgcatccc
      481 gagcgtgttc gacccgacgc tggccccgag cggcaagcac gtgatgtcga tgttcaccca
      541 gtgggtgccg cacggcttcg ccgccgcgcc ccacaccgcc gagctcgagg cctacgccga
      601 ccgcctgatc gcccggctcg acgagctcgc ccccggcttc acgtcgtcga tcctgcaccg
      661 ccagatcatc ggcccgcacg ccatggagac cacctacggc ctgatcggcg gcaacatctt
      721 ccacggcgag ctgtcggcga accagctgtt ccacatgcgg ccggccccgg gctacgccga
      781 cttcaccacc ccgatccgcg gtctgtacca ggcgtcgtcg gcgacccacg gcggcggcgg
      841 cgtcaccggc atccccgcgc tccaggcggt ccgccgcgtg ctgcgcgatt cgtagcggcc
      901 ggctactcgt tcagcgcccg gtgcaacaga tcgagcacgc catggctcgc ggcgtggcgc
      961 tccaggtacg cgcgatcgag cacctccccc tgcaccttga tgacgccacg gatgtctcgc
     1021 cattgccggt ccgagacccc gccgccctcg acgaaccaca gcagcttctg caggatgatg
     1081 tcctcggccg aggcacacgg gatccgttgg ccgtcgatat cgacgaagat ccggcgctgg
     1141 agctcctcgc gggccagcgg ctcgtcggcg ctgcagtaga tgtcgacctt ggtcatcgac
     1201 gccagctgga tgacattgaa tgcgccgcgt tcccggaccg cggccttcgc ggcgtcctcg
     1261 tcgatgtagt aggtgtcgac cagtgcggcg aacagcgcgc tgacatggcg gccgcgcaga
     1321 tccgcgacca ggtcgacgtc cgcggtcgct cgcggctcgc cgaggatcga actcgccacc
     1381 gatccgccga cgagccacgt gacgccgagc gaatcgagct tctgcccgac gtccagcgcg
     1441 acctcgagct caccgatcag catgatcgcc ctgacccggc agggtcaccg gccttgcagc
     1501 tcggggtccc aatgaaacgc gcggatcatc gtctcgcgat cgaggttgcg cgcggcgagc
     1561 cggagctcga tctcgcgcgg cgtggcatcc gggtagcgct tgcgcagctc ggcacgcacc
     1621 agctcgttga ccatcttgcc cacgccgaac actttgcgga gcttctgggc aggcgaggcc
     1681 tgacgccaga gccggaacat catgttctcg gcctctggtg aagtatccga agcgcccttg
     1741 gcggtgccgt ccatcatttc tcggaccggt aaactctacc gcggccagcc acgagaagtc
     1801 acccccagct ccaggcagcg gccttcgcgt agaacgcatg gaatctgcta ggtaatgtgc
     1861 ggtctgttga tgtcgcacag attcacgcca ggcgggcctc ggcgagctcg agggcgcggc
     1921 cgacgccgac ctcgacgtcg aacgcgtgga cgtcgagcac cgcggcccag tgatcgagcc
     1981 ggtgggcgag cgccgacagc gcgtcgctcg ccgcgcgcgc gagcggctcg ccgagctcgc
     2041 gatcgaggtc gggcgcgccg cggatcagcg cgtggtagac cggctcctcg ccgagctcga
     2101 gccggg
//
LOCUS       DASVOR010001339         5738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393102,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001339 DASVOR010000000
VERSION     DASVOR010001339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393102"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            426..2609
                     /locus_tag="VF516_25545"
     CDS             426..2609
                     /locus_tag="VF516_25545"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8111130.1"
                     /translation="MRSFLWPIAALLVLPSCGGSDGPVPAPFGLDQRPSNTTCLARPR
                     PVVNTGVKLQPMWSTLTFQEPLFLLQAPGDDNQWFVVQREGKVRAFARDATADSQVRD
                     FATVTVNSTGEGGLLGMAFHPQWPTRREVYLYYTRTPAAGDPAPVCPPAMTSQLISVI
                     SRFQSSDGTQLDTAPDEILRVGQPFTNHKGGTIEFGLDGMLYLGLGDGGSGDDPCGSG
                     QNTSSLLGKMLRFDVNAPRGMYNIPADNPFAGVSGARGEIWSYGHRNPFRWHFDRVSG
                     DLWVGDVGQNTWEEIDRVVKGGNYGWNLCEGFHVRGDTTHLCNMQQLGLLDPVVEHPR
                     TEAQSITGGAVYRGSAMPSLVGTYIYGDYITGNLWALTYDAGNKPVPKLIATVAPRTL
                     VAFGQGNDGEIYTVQISGTLSKLVPSGPQPPDSFPQLLSQTGCVDAKDPTRPAPGLIP
                     YDVAAPLWSDGADKQRYFAIPDGQNITINPDHDWDLPVGSVAMKTFSVGGKRVETRLF
                     MRHDDGGWAGYTYEWNDDGKDATLLPANKVKALGAGATWSYPSRTQCLQCHSQAAGGT
                     IGLETAQLNRDFVYTSTNRISNQLATLDHIGLFAAALAAPPDALPHLSDPAGKDPLEA
                     RARSYLHGNCSHCHRPNGGGQGMMDLRYAESLHDTNTCNQIETQGKVGTASLLIAPGA
                     PQDSIVSLRLHATDSKRMPPVAVSITDPLGTKLIDDWIRSLTACP"
     gene            2656..3060
                     /locus_tag="VF516_25550"
     CDS             2656..3060
                     /locus_tag="VF516_25550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111131.1"
                     /translation="MASANDIYRDLLALPEQERLQLVERVIHDLVDAARHRTATRATR
                     SANAVIGLWADEPEVADEIIEDALQQRETRPRLADRDLLVGAFRRFPFGPEYQVLAVR
                     VEASRTVVDIQLPESGEKATLPLEQTLECPQG"
     gene            complement(3453..5051)
                     /locus_tag="VF516_25555"
     CDS             complement(3453..5051)
                     /locus_tag="VF516_25555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111132.1"
                     /translation="MQAGSPGGGQPAKTMFGYAAPVVPGQQPRVGGAQPAGQPQRPGQ
                     PQPAAQPNPYGQQPGMPQANPYGQQPGMPQQQPNPYGQQPGMPQQANPYAQPQQPANP
                     YGQQPGMQQPNPYGQPQQQNPYGQQPGMQQQNPYGQQPNPYGQPPANPYAAPQQPAGY
                     PQQGGYPQQQNPYAQPQQEASGPLGDLASRLPHSAPGTILGIPVARLRDPGLQKKILF
                     LAGVALIASVVVPFSVSPLVFAWSGGGFEFFLWPIISGAAYLLLTAAPQDLRNKVPPV
                     VLQWIPFGVSYAGIFISHMGFGFALYLAAAAGGGVGFAGGAGGLYILGYSVLVFGLLA
                     RISQPQDQIARIVIAVGAACLIPSFLDSLRLFGMLGSFPILMVVHNLLWVLILALGVF
                     CILFVVPPVKLPPALQSVDAFGPLIAAALILWLPLQQVLIALVGIVHGHVLIGSLLML
                     GHGLLPILAYFGVLMMASPAAYEEAKAMFGRRGGQPPSGGYSGGGYPPQGGGYPPQGG
                     GYPPQQGGYPPQGGGYPPQQGGWQ"
     gene            5226..>5738
                     /locus_tag="VF516_25560"
     CDS             5226..>5738
                     /locus_tag="VF516_25560"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8111133.1"
                     /translation="MARRLPGHDAVRGEAIEVRIADTSVRAFPDESIAAAAIASGTRV
                     LARSLKYHRPRTFFCFEGHCSGCLVRLSGVPNLRACQAACAAGAEVEGQNAFPSAEVD
                     LLGAVDFLFSRGMDHHTLMTGSSVLNRLANKVVRQLSGLGKLPDRAAAELPEVAVRTV
                     DVCVIGGGPAG"
BASE COUNT          924 a         2027 c         1942 g          845 t
ORIGIN      
        1 gcccggcggc ccttgattgc ccggcggccc ctgcgacccc tgcccgcctg gcggcccttg
       61 atccccggtg cacgcggcga gcccgagcga aacgagcgag accaaggttc catatccgag
      121 caacctcatt gctgcctcca agcgaaagac cgttgttgcg ggcccagtgc aaggagtgaa
      181 cctgcgcaca agtggtcgtt ttcatgtgcg aatggcgaac gtcggccaga aattcgcgca
      241 gcgcgtgcag cacccctcgc gaacgcgagg aattttttcg ggatcgcagg accgacgagc
      301 tcgacgcgat cccccgcgcg cgaacgtccg ttcgtcgtga tcgagatcgc acgcgagccg
      361 gacgggatca gatgcgcagg tgaggcgtcg ggctcgcacc gcgcggcggc ggtatcgtcg
      421 cggccatgcg tagcttcctg tggccgatcg ccgcgctgct cgtgctgccg tcgtgcggag
      481 gcagcgacgg ccccgtgccg gcgccgttcg ggctcgacca gcgcccctcc aacaccacct
      541 gcctcgcccg gccgcgcccg gtcgtcaaca ccggcgtgaa gctgcagccg atgtggtcca
      601 cgctgacgtt ccaggagccg ctgttcctgc tgcaggctcc cggcgacgac aaccagtggt
      661 tcgtcgtcca gcgcgagggc aaggtccgcg cgttcgccag ggacgcgacc gccgatagcc
      721 aggtccgcga cttcgccacc gtcaccgtca actccaccgg cgagggcggc ctgctcggca
      781 tggcgttcca tccgcagtgg ccgaccaggc gcgaggtcta cctctattac acgcgaacgc
      841 ccgcggccgg cgatccggcg ccggtgtgcc cgccggcgat gaccagccag ctgatctcgg
      901 tgatctcgcg gttccagagc agcgacggta cccagctcga caccgcgccc gatgagatcc
      961 tccgggtcgg ccagccgttc accaaccaca agggcggcac gatcgagttc ggcctcgacg
     1021 gcatgctgta cctcggcctc ggcgacggcg gctcgggcga cgatccgtgc ggctcgggcc
     1081 agaacacgag ctcgctgctc ggcaagatgc tgcgcttcga cgtcaacgcg cccaggggca
     1141 tgtacaacat ccccgccgac aacccgttcg cgggcgtgtc cggcgcgcgc ggcgagatct
     1201 ggtcgtatgg ccaccgcaac ccgttccgct ggcacttcga tcgcgtctcc ggtgacctct
     1261 gggtcggcga cgtcggccag aacacgtggg aggagatcga tcgcgtggtc aagggcggca
     1321 attacggctg gaatctctgt gaaggcttcc acgtgcgcgg cgacacaaca cacctttgca
     1381 acatgcagca gttgggtttg ctcgatccgg tcgtcgagca cccgcggacc gaggcgcagt
     1441 cgatcaccgg cggcgcggtg taccgcggca gcgcgatgcc gagcctggtc ggcacgtaca
     1501 tctacggcga ctacatcacc ggcaacctct gggcgctgac ctacgacgcc ggcaacaagc
     1561 cggtgcccaa gctcatcgcg accgtcgccc ccaggacgct ggtcgcgttc ggccagggca
     1621 acgatggcga gatctacacg gtgcagatca gcgggaccct ctccaagctg gtgccgagcg
     1681 gcccgcagcc gccggacagc ttcccgcagc tgctatcgca gaccggctgt gtcgacgcca
     1741 aggatccgac ccggccggcc cccgggttga tcccgtacga cgtcgccgcc ccgctgtggt
     1801 ccgacggcgc cgacaagcag cgctacttcg cgatccccga cggccagaac atcacgatca
     1861 accccgacca cgactgggac ctgccggtcg gctcggtcgc gatgaagacg ttctcggtcg
     1921 gcggcaagcg ggtcgagacc cggctgttca tgcgccacga cgacggcggc tgggccggct
     1981 acacctacga gtggaacgac gacggcaagg acgcgacgct cctgccggcg aacaaggtca
     2041 aggcgctcgg cgccggcgcg acctggagct acccgagccg gacccagtgc ctgcaatgcc
     2101 actcccaggc cgccggcggc acgatcggcc tggagaccgc gcagctcaac cgcgacttcg
     2161 tctacacatc gaccaaccgc atctcgaacc agcttgccac gctcgaccac atcggcctgt
     2221 tcgcggccgc gctcgccgcg ccacccgacg ccctgccgca cctgtccgat ccggccggca
     2281 aggacccgct ggaggcccgc gcgcgcagct acctgcacgg caactgctcg cactgccacc
     2341 gccccaacgg cggcggccag ggcatgatgg acctgcgcta cgccgagtcg ctgcacgaca
     2401 ccaacacctg caaccagatc gaaacgcagg gcaaggttgg cacggcgagc ctgctgatcg
     2461 ccccaggcgc gccgcaggac agcatcgtgt cgctgcggct ccacgccacc gactccaagc
     2521 gcatgccgcc ggtggcggtg agcatcaccg atccgctcgg cacgaagctg atcgacgact
     2581 ggatccgctc gctgaccgca tgcccgtgac gggagcgagt cgtccgcagc ccggcgggcg
     2641 cgctataccg gcgccatggc atcggccaac gacatctatc gcgatctgct ggcgctcccc
     2701 gagcaggaac gcttgcagct cgtcgagcgt gtcatccatg acctggtgga tgccgcccga
     2761 catcgcacgg cgacccgggc cacgcgctcc gcaaacgccg tgatcggcct gtgggctgac
     2821 gaacccgagg tcgcggacga gatcatcgag gacgccttgc agcagcgcga gacgagaccg
     2881 cgcttggccg accgcgactt gctcgtcggt gcgttccgcc gcttcccatt cggcccggaa
     2941 tatcaggtcc tcgcggtccg agtggaggcc agcaggaccg tagtggatat tcagctcccg
     3001 gagtccggcg aaaaggcaac acttccgctg gagcagactc tcgaatgccc ccaaggctga
     3061 ataagggtcg attccggcga tctgcatcgc gttgcttgcg acgatcgcgt cgatgcgctg
     3121 gcacacacca cgacgatgct cgcgtagccg ccgtcgacgc cctggacctt gaccttcacc
     3181 ttgaccccac ctttgacgtt cgacggcaac gtagacgtcg accccaccct tgaccttgca
     3241 cgttgactcc ggagcgaagc aaccctcggc cggcggagcc gatacccagg cgctcgaggt
     3301 ttatcgcgga gcttccctcg atgatcgaag gtcgacgtcg cggtcgatgg tggagtcgac
     3361 gtccaacgtc gccctccacg tccacgtttg gggtcaacct cgacgtcaaa ccgtcaacgt
     3421 caaccctccg cttgcgccgc tttcgttgag cgttactgcc agccgccctg ctgcggcggg
     3481 tagccaccgc cttgcggcgg atagccgccc tgctggggcg ggtagccgcc accttgcggc
     3541 gggtaaccac cgccttgtgg cgggtagccg ccgccggagt aaccgccgct cggcggctgg
     3601 ccgccgcgcc gcccgaacat cgccttggct tcctcgtagg cggccggtga cgccatcatc
     3661 agcacgccga agtacgcgag gatcggcaag aggccgtggc cgagcatgag cagtgaaccg
     3721 atcaggacgt ggccgtggac gatgccgacc agggcgatca gcacctgctg gagcggcagc
     3781 cacaggatca gcgccgccgc gatcagcggg ccgaacgcat cgaccgactg cagcgcgggc
     3841 ggcagcttga ccggcggcac gacgaacagg atgcagaaca cgccgagcgc caggatcagg
     3901 acccacagga ggttgtggac caccatgagg atcgggaacg agccgagcat gccgaacagg
     3961 cgcaggctgt cgaggaacga cgggatcagg cacgcggcgc cgacggcgat cacgatgcgc
     4021 gcgatctgat cctgcggctg ggagatccgc gccagcaggc cgaacacgag caccgagtag
     4081 cccaggatgt agagcccgcc ggcgccaccg gcgaacccga ccccaccgcc ggccgctgcg
     4141 gcgaggtaca gcgcgaagcc aaagcccatg tgcgagatga agatgcccgc atacgagacg
     4201 ccgaacggga tccactgcag gaccaccggc gggaccttgt tgcgcaggtc ctgcggcgcc
     4261 gcggtgagca ggaggtacgc ggcgccggag atgatcggcc acaggaagaa ctcgaagccg
     4321 ccgcccgacc acgcgaacac cagcgggctg accgagaacg ggacgacgac cgatgcgatg
     4381 agcgcgacgc cggcgaggaa caggatcttc ttctgcaggc ccggatcgcg cagccgcgcc
     4441 accgggatgc cgaggatcgt cccaggcgcc gagtgcggca gcctgctggc gaggtcgccg
     4501 agcggacccg acgcctcctg ctgcggctgc gcgtacgggt tctgctgctg cggatagccg
     4561 ccttgctgcg gataacccgc cggctgctgc ggcgccgcgt acgggttcgc cggcggctgg
     4621 ccgtacgggt tgggctgctg gccgtacggg ttctgctgct gcatccccgg ctgctggccg
     4681 tacgggttct gctgctgcgg ctggccgtac gggttgggct gctgcatccc cggctgctga
     4741 ccgtacgggt tcgcaggctg ctggggctgc gcgtacgggt tcgcctgctg gggcattccg
     4801 ggctgctggc cgtacggatt gggttgctgc tggggcatcc ccggctgctg gccgtacggg
     4861 ttcgcctggg gcatcccggg ctgctggccg tacggattgg gctgcgccgc tggctggggc
     4921 tgtcccggac gctgaggctg acccgccggc tgtgcgccgc cgacgcgcgg ctgctggccg
     4981 gggacgacgg gagcggcata gccgaacatc gtcttggccg gttggccgcc accgggtgag
     5041 cccgcctgca tcggtggcat cggtcggccg cacgtaccgc acgctggcgc gttcatcagc
     5101 acagccgcgc cgcagtacgg gcatggcctg gttggtgtag ccattcggga cccctatcct
     5161 ttcagacgga aaggtatctc acgccgagca gcctctgcaa ccgagtggtg tgttatgtgt
     5221 cgtacatggc gcgccggctc ccgggacacg acgccgtccg cggcgaagcg atcgaggtgc
     5281 ggatcgccga cacctcggtt cgggcttttc ccgacgagag catcgcggcg gccgcgattg
     5341 ccagcggcac ccgcgtgctc gcacgctcgc tcaagtacca tcgcccgcgg acgttcttct
     5401 gcttcgaggg tcactgcagc ggctgcctgg tgcggctgtc gggggtgccc aacctgcggg
     5461 cctgtcaggc ggcgtgcgcc gcgggcgcag aggtcgaggg ccagaacgcg ttcccgtcgg
     5521 ccgaggtcga cctgctcggc gcggtggact tcctgttctc gcgcggcatg gaccaccaca
     5581 cgctgatgac cgggtcgtcg gtgctcaacc ggctcgccaa caaggtcgtg cgccagctgt
     5641 cggggctggg caagctgccc gatcgcgcgg cggccgagct gcccgaggtc gcggtccgca
     5701 ccgtcgacgt ctgcgtgatc ggcggcggcc ccgccggg
//
LOCUS       DASVOR010001340         5414 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_130433,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001340 DASVOR010000000
VERSION     DASVOR010001340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130433"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="VF516_25565"
     CDS             complement(<1..792)
                     /locus_tag="VF516_25565"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8111134.1"
                     /translation="MLRFATGPETIRLGDLEVRRLGFGAMRLPGKDVWGEPDDPAAAR
                     AVLRRAVELGINLIDTAWYYGPHVANRLIAEALHPYPSDLVIATKLGGRRLPDKSWAP
                     YLRPEELRAGAEHDLRDLRLEQLPVVHLRWIEGTGVAFGEALDAMIEMQREGLIRHIA
                     LSNVSVEQLEYALTRTPIACVQNLFNVGGGGGPIPVADRPEAVLAACEAHGIAFLPFF
                     PLAIGTLTASSGALAAVASRHGCTPAQLALAWLLARSPVMLPIPGT"
     gene            complement(869..1243)
                     /locus_tag="VF516_25570"
     CDS             complement(869..1243)
                     /locus_tag="VF516_25570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111135.1"
                     /translation="MPEVGRVVRPGGLIAPVTYFNAELDGEAGALLAGFYADIVPHWP
                     GGRTHVNNRYRDLTFPWPAVEPPAIALTAPVNARRAVGYVTTWSATARLVAAGGARGW
                     SGWGASWPSAGRATSRARSAGR"
     gene            complement(1395..2198)
                     /locus_tag="VF516_25575"
     CDS             complement(1395..2198)
                     /locus_tag="VF516_25575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111136.1"
                     /translation="MKVENGEKSMKITLLWKSALVVSALLVFLFAILGEAAPMGEAGP
                     QVATPPSKVTTVTFRPFSEFLAAQRIGGGDLFFPPVKNYVGWTTNQNLKKATLFALVD
                     YAGLADDFLNGQLGTQVDGLVTETELPNGSARITVALTATNALGFAQVVSQILDNNTD
                     LPFLNTPTFFGNKAQDVANGAEAAVGDVSFLVTFTNSEPGAPLPNLIDVITNINKHPE
                     FKPVNVTFIATVPGPCENGTRDVLHVDQEGAPGNNEGTQIVEVVSEPCS"
     gene            complement(3554..4294)
                     /locus_tag="VF516_25580"
     CDS             complement(3554..4294)
                     /locus_tag="VF516_25580"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8111137.1"
                     /translation="MTAFADHFSELAARYAQYRPHYPPALVDALAGASPARGVAWDIG
                     CGNGQLSVALAARFDRVIATDPARAQLDHAERHPRVEYRCEPAEASSLPDAGVDLAVA
                     AQAAHWFDWPRFVPEVGRVVRPGGLIALVTYFNAELDGEAGALLAGFYADIVPHWPGG
                     RTHVNNRYRDLTFPWPAVEPPAIALTARWTRDELFGYVTTWSATARLVAAGGGARLER
                     LGRELAERWPGNEPRENRWPLTIKLTRR"
     gene            4390..5193
                     /locus_tag="VF516_25585"
     CDS             4390..5193
                     /locus_tag="VF516_25585"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEX8111138.1"
                     /translation="MLTRRRWLAGGLAAALALVTRRASAIGPGSKFTFGQLTLGRGDL
                     GPRPGALRRLAWEIEKRTSIDVQLDPVIVTPTSETLHETPFLYLSGERELELPSAAGI
                     EALRRFLTFGGFLLIDSAEGSTDGAFDSSVRKLMAAVFPPVGSAKPLEVVPSDHVVYK
                     SFYLLDRPLGRLAIAPAMEAVIRDGRIVAAYVANDLGGAWQRDDFGNYDFPCQPGGER
                     QRELAFRMGVNLVMYALCLDYKADQVHVPFIMKRRRWKPDDGALTPVKP"
     gene            5219..>5414
                     /locus_tag="VF516_25590"
     CDS             5219..>5414
                     /locus_tag="VF516_25590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111139.1"
                     /translation="MSIVLFSPLMRVSLAAAAALLIIATIVAVRRGHGGLGALLGVSA
                     LIGGGALAAEALIALTVRIAA"
BASE COUNT          855 a         1881 c         1842 g          836 t
ORIGIN      
        1 ggtgcccggg atcggcagca tcaccgggga gcgcgcgagc agccaggcga gcgccagctg
       61 cgccggggtg cagccgtggc gcgaggcgac cgcggccagc gcgccgctgc tcgcggtcaa
      121 cgtgccgatc gcgagcggaa agaacggcag gaacgcgatg ccgtgcgcct cgcacgcggc
      181 gagcacggcc tcggggcggt cagcgaccgg gatcggcccg cccccgccgc cgacgttgaa
      241 caggttctgg acgcacgcga tcggcgtccg ggtcagcgcg tactcgagct gctcgacgct
      301 gacgttgctg agcgcgatgt ggcggatcag cccctcgcgc tgcatctcga tcatcgcgtc
      361 gagcgcctcg ccgaacgcga cgccggtgcc ctcgatccag cgcaggtgga cgaccggcag
      421 ctgctcgagc cggagatcgc gcaggtcgtg ctcggcgccg gcgcgcagct cctcgggccg
      481 gaggtacggc gcccacgact tgtcgggcag ccggcggccg ccgagcttgg tcgcgatgac
      541 caggtcgctg ggatacgggt gcagcgcctc ggcgatcagc cggttggcga cgtgcgggcc
      601 gtagtaccac gcggtgtcga tcaggttgat accgagctcg acggcccggc gcagcaccgc
      661 ccgcgcggcc gcggggtcgt cgggctcgcc ccagacgtcc ttgccgggga gccgcatcgc
      721 gccgaacccc aggcgccgga cctcgaggtc gccgagccgg atggtttcag ggccggtggc
      781 aaaccgcagc atggctgcac gatgcccgat cccgcccggc gccgccacgg ccgcggcgcg
      841 gatcagacgc gcctcgcgag ctcgatcgtc agcggccagc ggatctcgcg cggctcgttg
      901 cccgaccagc gctcggccaa ctcgcgcccc agccgctcca gccccgcgcc ccgcccgccg
      961 cgaccagccg cgcggtcgcc gaccaggtcg tgacgtagcc gacagctcgt cgcgcgttca
     1021 ccggtgcggt cagggcgatc gccggaggct cgaccgccgg ccacgggaac gtgaggtcgc
     1081 ggtagcggtt gttgacgtgc gtccgcccgc ccggccagtg cgggacgatg tcggcgtaga
     1141 acccggcgag cagcgcgccg gcctcgccgt ccagctcggc gttgaagtag gtgaccggcg
     1201 cgatcaggcc gcccggccgg acgacccggc cgacctcggg cacgaaccgc ggccagtcga
     1261 accagtgggc ggcctgcgcc gcgaccccgg cgtcgggcag gctcgacgcc tcggcgggct
     1321 cgcagcggta ctcgacccgc ggcccggcgc aattcgagat atcttatctg tcggacacct
     1381 gcacctgaag ccgcctagct gcagggctca ctaacaacct ccacaatttg tgtgccttca
     1441 ttgttcccag gagcgccctc ctggtccaca tgcaagacgt ctcttgtccc attctcacat
     1501 ggtcccggta ctgtggcgat aaaagtgaca ttgaccggct tgaattcagg atgtttattg
     1561 atattggtaa taacatctat aaggttgggc aaaggagccc cgggctccga gtttgtgaag
     1621 gtaaccaaaa aggagacgtc tccgactgcg gcttccgcgc cgttggctac atcctgggcc
     1681 ttattgccaa agaaggttgg tgtattgaga aaaggtaaat ctgtattgtt atccaggatt
     1741 tgagagacca cctgggcaaa tcccagtgcg ttggtggcgg tgagagccac cgtgatccga
     1801 gcgctgccgt tggggagctc ggtttcggtg acaaggccat ccacctgtgt acccaattga
     1861 ccatttaaaa aatcgtccgc caggccagca taatccacca atgcgaagag agtggccttt
     1921 tttaaattct gattagttgt ccaacccaca tagttcttca cgggtgggaa gaagaggtct
     1981 cctcctccaa ttcgttgggc agcgaggaac tcggagaacg gccggaaggt caccgtggtc
     2041 accttcgagg gcggtgtggc cacctgagga ccagcttctc ccatgggcgc tgcttcgccg
     2101 aggatagcaa atagaaacac cagtaacgcc gaaacaacga gagcgctctt ccagagaagc
     2161 gtgattttca tcgatttctc cccattttcc actttcatga ctccctcctg gtagatgtac
     2221 tcgtcaactc gttcatcgga taggctttga ggacaacgcc cgtcggcagc ggagcaacac
     2281 ctcactgcca actgtcgagg taaactatgc tggctggcga aacataagca aatgagaaaa
     2341 tcctatggat gggggtaggg acggcgtgca gagtgagcga caccgactgc acctgcgagt
     2401 cctgcacggc tgccgcgtgc gaggaccagg gcacgccggt gaccatcatc ccggctgact
     2461 gacgccatcg tacggcgcgc ggctgcggat gggcgcatcc cgcggggtgc acccagcgcg
     2521 gtcaccgcgc ccgtccccgg agcagcgcga accccgaggg ctccctcgcg ctgctcggcc
     2581 cccgaggagc ggctgtacgc agccggagat cacggcacgc tgctcgagcg ccgcgacggg
     2641 cgctggcgag tgatcgcgac gcactccagc gccgacccgc gctgattcca cggccgcctc
     2701 gcggtcggcc tcggtggcgc cgttatcgcc tcgggaccac ctcggtcagc ttgtgccact
     2761 gtatctgttc tttcgagtct ccccggagcg ccgccacgac atccgttctg tcggatctcc
     2821 gcgccttcgt gaccgccgga gcagcgatgc tcgatctgtg gccagatcgc acgccgtgct
     2881 cacgcgcgcg agcgcacacg cacgcacgac tgctaacacc gcgacaccaa agatctctga
     2941 cttggcaaga aactcgcttt cgccggaatc ccaggtcatc gatgtgacac tcgagtttgg
     3001 ccatcaccgg ccatcactca tgcctgtcgc aaagaacagc gggaaagttc cggcaagcgc
     3061 cggaacggat ggatgaacag gaggggttgg tcaatggacc gatacgcgct caggctgatc
     3121 ctggctctcg ccgtcctcgg gctaaccttg acatcgaccg gcgccatggc cgagacatcc
     3181 tccgtcttgc cgcccggcga ccaaccaaac ggtcgcaccg acggtgaatc ggtggccgcg
     3241 tggtggcgcg gggcgctctc acagccggcg gggacgaacc ccctcctcga ctcgaacagt
     3301 gccttctccg cgaacgagca agagagcgag gtctggtcct cgttggctcc gccggggaga
     3361 tggtcactcg cagctgtgcg cggtctttca ggatccgccc gagcggtcga ccgaagcgtt
     3421 cgtccgtgac caggggtctt ctcgatccgg tcgtgatccg cgagatgctc gatgccgcga
     3481 acgactgcag gccgccatcg accaggccca ggatcgagcg acggtcgaga agcatcggga
     3541 ctggctaagg cgcctagcgc cgcgtcagct tgatcgtgag cggccagcgg ttctcgcgcg
     3601 gctcgttgcc cggccagcgc tcggccagct cgcgccccag ccgctccagc cgcgcgccac
     3661 cgcccgccgc caccagccgc gcggtcgccg accaggtcgt gacgtagccg aacagctcgt
     3721 cgcgcgtcca ccgcgcagtc agggcgatcg ccggaggctc gaccgccggc cacgggaacg
     3781 tgaggtcgcg atagcggttg ttgacgtgcg tccgcccgcc cggccagtgc gggacgatgt
     3841 cggcgtagaa cccggcgagc agcgcgccgg cctcgccgtc cagctcggcg ttgaagtagg
     3901 tgaccagcgc gatcaggccg cccggccgga cgacccggcc gacctcgggc acgaaccgcg
     3961 gccagtcgaa ccagtgggcg gcctgcgccg cgaccgcgag gtcgaccccg gcgtcgggca
     4021 ggctcgacgc ctcggcgggc tcgcagcggt actcgacccg cgggtggcgc tcggcgtggt
     4081 cgagctgggc ccgggccgga tcggtggcga tcacgcggtc gaaccgcgcc gcgagcgcga
     4141 ccgacagctg gccgttgccg cagccgatgt cccacgccac cccgcgcgcc gggctggcac
     4201 cggcgagcgc gtcgaccagc gcgggcggat agtgcgggcg gtactgggcg tagcgcgccg
     4261 cgagctcgga aaagtgatcg gcgaacgcgg tcatgcgctt gcgatgcgtc atcctcgcac
     4321 ggcgttgcgc ggtcgaccaa ggcggcgcgg gcactggccc cgggcaacgc cgagcgctat
     4381 tttcggacca tgctgacacg gcgccgatgg ctcgccggcg gcctggccgc tgcgctggcg
     4441 ctcgtcaccc ggcgcgcgtc cgcgatcggc ccgggctcga agttcacgtt cggccagctc
     4501 acgctcggcc gcggcgacct cggcccgcgg cccggcgcgc tgcgccggct ggcctgggag
     4561 atcgagaagc gcacgtcgat cgacgtccag ctcgatccgg tgatcgtcac gccgaccagc
     4621 gagaccctgc acgagacccc gttcctgtac ctgtccggcg agcgcgagct cgagctgccg
     4681 agcgcggcgg ggatcgaggc gctgcgccgg ttcctcacgt tcggcggctt cctgctgatc
     4741 gactcggccg agggctcgac cgacggcgcg ttcgacagct cggtgcgcaa gctgatggcc
     4801 gcggtgttcc cgccggtcgg ctccgccaag ccgctcgagg tcgtgccgag cgatcacgtg
     4861 gtctacaagt cgttctacct gctcgatcgc ccgctcggcc ggctcgcgat cgccccggcg
     4921 atggaggcgg tgatccgcga cggccggatc gtcgcggcct acgtcgccaa cgacctcggc
     4981 ggcgcgtggc agcgcgacga cttcggcaac tacgacttcc cgtgccagcc cggcggcgag
     5041 cgccagcgcg agctcgcgtt ccgcatgggc gtcaacctgg tgatgtacgc gctgtgcctc
     5101 gactacaagg ccgatcaggt ccacgtgccg ttcatcatga agcgccgccg gtggaagccc
     5161 gacgacggcg cgctgacccc ggtcaagccg tgacggcgat gtcggcaggg gcccggtaat
     5221 gtcgatcgtc ctgttcagcc cgctgatgcg ggtgtcgctc gccgccgccg ccgcgctgct
     5281 gatcatcgcg accatcgtcg cggtccgccg cggccacggc gggctcggcg cgctgctcgg
     5341 cgtgtccgcg ctgatcggcg gcggcgcgct cgcggccgag gcgctgatcg cgctgaccgt
     5401 gcggatcgcc gcgc
//
LOCUS       DASVOR010001341         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_276006,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001341 DASVOR010000000
VERSION     DASVOR010001341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276006"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF516_25595"
     CDS             complement(<1..216)
                     /locus_tag="VF516_25595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111140.1"
                     /translation="MRIGSASETLRDRLTKEKHMRFLEMALGLMVFSACSGELGDADR
                     ATGPDVGAADGAAAFDLLRAGHHGDRGV"
     gene            495..944
                     /locus_tag="VF516_25600"
     CDS             495..944
                     /locus_tag="VF516_25600"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HEX8111141.1"
                     /translation="MRRLFKGLLLALAWIAGVFALMQIVPYGRTHSNPATIKEPAWDS
                     PRTRELAVRACFNCHSNRTRWPWYASVAPFSWVVQADVEGARDIINFSEWNRPYDLAL
                     YSGQSIRTGNMPPIKYSMAHPEANLTEQEARELASGLDAMLRPPGQR"
     gene            987..1811
                     /locus_tag="VF516_25605"
     CDS             987..1811
                     /locus_tag="VF516_25605"
                     /inference="COORDINATES: protein motif:HMM:NF020604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stf0 family sulfotransferase"
                     /protein_id="HEX8111142.1"
                     /translation="MRPIRSYLIWFTQRVGSTMLTQALEDTGVAGRPREWLNVASVRE
                     LLARHDAATVHDLRDALWREATTDNGIMGTKYGMTEALHSELTALLAGLVPELPDPDG
                     RHAWNALYPDCMHVFMTRRNKIRLAVSWWRAIKSGEWHRPSRRTRTVFDPASGPIDRR
                     EPPSRADIADLYDYHAIAHLLAEASLREAAMQERFDRWGVVPYTVVYEDLIAAYEPTM
                     RALLEFLQIPGRDAIELAAPAFERLADEVSEAWVQRFHRELDTRLAEQARAPAPPR"
     gene            1886..>2436
                     /locus_tag="VF516_25610"
     CDS             1886..>2436
                     /locus_tag="VF516_25610"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8111143.1"
                     /translation="MHPPRTRPPAETLSWVERTLGPGARVVGWRRLTGGITSSVHRLT
                     VEHNGRRRPFVLRRWVPDREHEDYAAGAVASETAALTALERSDVPAPRVIGSTTDVAH
                     AGPAVLMTRVPGHVHRMPRDRERWLQQMAHTLTRIHALDIDARPFQSWLDRSRLSPLP
                     DASRPDIWREAIALVAEERRPTQT"
BASE COUNT          373 a          874 c          836 g          353 t
ORIGIN      
        1 cacgccgcga tcgccgtgat gccccgcgcg cagcagatcg aacgcggccg cgccatcggc
       61 ggcgccgacg tccggcccgg tcgccctgtc ggcgtcgccc agctctccgc tgcacgcgct
      121 gaacaccatc agtccgagag ccatctcgag aaaccgcata tgcttctcct tggtcaggcg
      181 gtcccgcagc gtctccgacg ccgacccgat gcgcaatcga ctcgtgcacc gctcacgcgc
      241 tttccacgac ggatgtggat cgccactcgc gatccgcgtg aggcactcct gcggctgtgc
      301 gcgacctgca cagccgggct gcgagccgcc acgtagatcg gcggggcgcg atgactcggt
      361 cggtgggggc gcggcgacgg atcgtcgcac ctcctcgcac acccacggat cgacgctcgg
      421 cgccgacgac ctgcggcaac gtgacacgag ccaggcatcc tcgtcctggc atccgaggcg
      481 gtatgaaggc tgacgtgcgg cgtctgttca agggtctctt gctggcactc gcctggatcg
      541 ccggtgtctt tgcgctgatg cagatcgtcc cctatggccg cacgcattcc aatccggcca
      601 ccatcaagga gccagcgtgg gactcgccgc gcacgcgcga gctggccgta cgcgcctgct
      661 tcaactgcca cagcaaccgg acgagatggc cctggtacgc gagcgtcgcg ccgttctcgt
      721 gggtcgtcca ggccgatgtc gaaggcgcgc gtgacatcat caacttctcg gagtggaacc
      781 gcccgtacga tctcgcgctg tactccgggc agtcgatccg gaccggcaac atgccgccga
      841 tcaagtacag catggcccat ccggaagcga acctgaccga gcaggaagcg cgcgagctcg
      901 cgagtggcct cgacgcgatg ctgagaccgc ccggacagcg ctgagggcct gcgggcccgg
      961 tgacgcgctg tcatactcgg tcgcccatgc gcccgatccg gtcctacctc atctggttca
     1021 ctcagcgcgt cggcagcacg atgctgacgc aggcgctcga agataccggg gttgccgggc
     1081 ggcctcgcga atggctcaac gtcgcgagcg tccgcgagct gctcgctcgc cacgatgcgg
     1141 caaccgtcca cgacctgcgg gacgccctgt ggcgggaagc gaccaccgac aacggcatca
     1201 tgggcacgaa gtacggaatg accgaagcat tgcattccga gctgacggca ctcctggcag
     1261 ggctggtgcc ggagctgccg gatcccgacg gccggcacgc gtggaacgcc ctgtaccccg
     1321 attgcatgca cgtcttcatg acgcggcgga acaagatccg gctcgcggtc tcgtggtggc
     1381 gagcgatcaa gtccggcgag tggcaccggc ccagccgccg cacacggacc gtgttcgatc
     1441 cggcctcggg gccgatcgat cgccgggagc cgccgtcccg agcggacatc gccgacctct
     1501 atgactatca cgcgatcgca cacctgcttg ccgaggccag cctgcgcgag gccgcgatgc
     1561 aggagcggtt cgatcggtgg ggcgtggtgc cgtacacggt ggtctacgag gacctgatcg
     1621 ccgcgtacga gcccacgatg cgagcgctgc tcgagttcct gcagatccct ggccgggacg
     1681 cgatcgagct cgccgcaccg gcgttcgagc ggctcgccga cgaggtgtcg gaggcctggg
     1741 ttcaacgctt ccatcgcgag ctggacactc gtctggccga gcaggcgcga gcaccggctc
     1801 ccccgcggtg atggaccagc ggccggcccg ctccgcacaa gccggtggct ggcggtatcg
     1861 gccgcgctcg gaggacaatg gcggggtgca tccgccccgg acgcgaccgc cggccgagac
     1921 cctgtcgtgg gtcgagcgga ccctcgggcc cggcgcccgg gtggtcggat ggcggcggct
     1981 caccgggggg atcacgtcgt cggtccaccg gctgacggtg gagcacaacg gccggcgcag
     2041 gccgttcgtc ctgcgccggt gggtgcccga ccgcgagcac gaggactacg ccgctggcgc
     2101 ggtggcgtcg gagacggcgg ccctgaccgc cctcgagcgc agcgatgttc cggcgccgcg
     2161 cgtgatcggc tcgaccaccg acgtggccca cgccggtccg gccgtcctca tgacgcgtgt
     2221 gccggggcac gtccaccgga tgcctcgcga tcgagagcgc tggctgcagc agatggcgca
     2281 cacgctcacg cgcatccacg ccctcgacat cgacgccaga ccattccaat cgtggctcga
     2341 tcgcagccgg ctgtcgccgc tgccggacgc gtcgcgcccc gacatctggc gggaggcgat
     2401 cgcgctggtc gccgaggagc gccggccgac gcagac
//
LOCUS       DASVOR010001342         9887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_451976,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001342 DASVOR010000000
VERSION     DASVOR010001342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_451976"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VF516_25615"
     CDS             <1..535
                     /locus_tag="VF516_25615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111144.1"
                     /translation="LSAGPAAAQPEAGSGDPLGPVRCYATATRDSLAPMEQARQLCIG
                     AVDESPARCFARAAAREFADAQAIQLCAGTTSLAPAMCADRLKPSGLDDGTIVAYCAA
                     LPWPLFPLAGPGAPACVLGARGRTGLSDTDAVRLCGGSTSTAPVDCFAWGAANTYLSD
                     HDLVELCLPVGLYPYPP"
     gene            complement(547..2520)
                     /locus_tag="VF516_25620"
     CDS             complement(547..2520)
                     /locus_tag="VF516_25620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8111145.1"
                     /translation="MSDQPSDKSTDAGPPIAKATRAHKDVDTEQDEHKRREFMHAMLA
                     DLRALDRMLREGRFETGPRRIGAEQEMFLIDRHWGPARGSLQMLDKLRDPHYTTELGL
                     FQLEANADPQLFTGDGLSRMEKQLDHLVGKARAAAAELGLAPVLMGILPTLRKSDLGL
                     EGMVPSPRYRALNQAMADLRGGTFEFSINGTDELIVRHDSVMLEACNASFQVHLQVTP
                     VEFPRMYNLAQVLAGPMVAVAANSPLLFGRRLWAETRIALFRQAVDTRSHKHHLRETE
                     ARVSFGTRWVRESVLEIYQEDIARFRTLVGTDLDEDPMAKLARGETPLLKALRLHNGT
                     VYRWNRACYGVMDGKAHLRIENRVMPAGPTVLDEIANGALWFGVMAELGAREEDVTRR
                     IDFDQAGANFYAAAREGLGVHFIWLDGEDIAASHLVLDRLLPLAEAGLRRQGIVEDDI
                     KRYLSVIDARVRTGRTGARWLLSSWNALKDKATSGERSSALVAATLQRQQTGRPVAEW
                     ERARLDESAAGETSYQRVDHYMTTDLFTVHASDPIEMVVNLMSWERIRHVPVEDKDHR
                     LVGLVTYRAVLRFLAGGGSLHDTPVSAIMRTDVLTVTPEARTLDAIALMRRYRYGCLP
                     VLQDGALVGILTEEDFMNVASKLLEQQLGEPRP"
     gene            2743..3561
                     /locus_tag="VF516_25625"
     CDS             2743..3561
                     /locus_tag="VF516_25625"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8111146.1"
                     /translation="MGRFRYYLPSAVTIVGIAFAAIAVQRAVEGQYRTAAWWAMYCLL
                     TDNIDGAIARRLRASSPFGAQLDSFADFFSFGVAPATVFYAFFASTPGAGWSDPVHRG
                     VLSLLVLLLITCVAIRLARFNVTHSVAGAKRFFVGWPTTYIGGMLAALFTLALKYGDP
                     AWAADDPGGDQLRLFGDVRLDGMMRLLPWLLLPASLVMISKLRMPRAALTGRRWVDIL
                     LAVTMVVGYGLGLARRLPEYLVAGGVVYMAISLRYHLISRGASPLPPLFPADDR"
     gene            3679..4803
                     /gene="cfa"
                     /locus_tag="VF516_25630"
     CDS             3679..4803
                     /gene="cfa"
                     /locus_tag="VF516_25630"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HEX8111147.1"
                     /translation="MSAKRIVAELLGRAGVGTDGAQPWDLHVLDERLYGRVIAQGSLG
                     FGDSYVDGWWECARIDELIHRLLSHDLEAELGTDWGVRLQLLKARLLNLQAPSRAPAV
                     AAHYDLGNEFFERVLGKTVMYSCAYWRRAGDLDAAQLDKIDLVARKLEVAAGDHVLDL
                     GCGWGAIAEHLARNCGCRVTAVTISPAQAEYVRRRCTGLPVEVIEADYRDARVASGGP
                     YDKLACVGMMEHVGRKNYRNFFSLCARLVKPGGLCLVHTIGTSVTTDVTDPWIERRIF
                     PGSALPSVADVAHAAEGQLTLEDWHNFGADYDRTLLAWHENFAAYAASGELQRGERFN
                     RTWQYYLLSFAGCFRTRRTIQLWQMVFSRDGRKGGYVSVR"
     gene            4787..5737
                     /locus_tag="VF516_25635"
     CDS             4787..5737
                     /locus_tag="VF516_25635"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8111148.1"
                     /translation="MSRFADLTAAAADRAATLGGMLEALYLSRFEIPPIDAADAARGV
                     QRIELEHATVRLRIAGDGAHPIVIVPDPPNTLEHYDELIDLLLARGGVRVICFEVPGF
                     GLSFPRSLTFSFSAAEYTDVIVELIDRLALRDVTLVISCIGGYVGLMAARLRPDLIGR
                     LVLCQTPAVHHMIAWARRFDKLGLLGLPLVGQAIMWLLKDPATRFWYSAALPIGADVA
                     RYQAPALAAQRIGGPYALASGIQAMRRLDVRELAGVTQPVTLAWGHGDRTHARTDPRS
                     LLAVVPHARFVSFERCGHFPDLEDPQRFTELVFDSLEAGG"
     gene            5734..6780
                     /locus_tag="VF516_25640"
     CDS             5734..6780
                     /locus_tag="VF516_25640"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8111149.1"
                     /translation="MTGRPDRPYDPARDHALLIQLAQRLRAAGCFARCDHRYARRLAF
                     LVPLFATGFAALLAVPRGPIWALAIAGTAFVSVQLGIVSHDAGHRAVHRRRGWNDLWG
                     HVGMTFVCGLSFTHWRIVHDTHHRHSQDELRDPDMQYAAVFSVHGRGAAEKRGLARVM
                     RPLQHFYFWPLGALYSWSLRWDSIARLFRDPDRTRVDRWVLPGHYALWLVGPALIVGP
                     AAAVVSYVLVSTLIGLYLVLIFAPNHMGMPSRRAGERWTYLEQQIITSRDLTVGRLLA
                     VVLGGLEHQIEHHLFPNIGQPRLRAARAIVTAFCVEHGLSHHEVGFFAAHRAVLGQLA
                     CMARFPVPAPEGLT"
     gene            6777..7844
                     /locus_tag="VF516_25645"
     CDS             6777..7844
                     /locus_tag="VF516_25645"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8111150.1"
                     /translation="MKRRPADPLYVKLSREVARAGYFTRDRYGYLWRGVLLIALFVIT
                     YVSLLFIRNLAFVSFALVLMAQVVTQIAYLSHDAGHNAITRDRRWIQIVGHFGMTFLT
                     GFSFSWWMHSHDNHHGKLNERDHDLAMKYSTVLAVHEDAVRQKRGARRAVLRWQGHYV
                     WLLMPFYHFAMMFDGFLWIARNPRTTRIDQLVLPGYIALWFIVPVPFIGWKLAAIHWL
                     AESMIASIYLVLTFIVHHVGKKVILPSDEPAMTLLRQQLETTRTLATWRIFDWYYSGL
                     NYHIEHHLFPWVPHWRYRKMRFLVQDFCRREGIAYREQGFWSAVVEVFVHLRRVGRCA
                     DLDAPAALSTLRSAHEPQTEG"
     gene            7949..8761
                     /locus_tag="VF516_25650"
     CDS             7949..8761
                     /locus_tag="VF516_25650"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8111151.1"
                     /translation="MPFIGVHAAAFVGVAVLGWSWQGLLLAVLFYFVRMFGITAGFHR
                     YFAHRSFKTSRTFQLLLAILGTTSTQKGVLWWAAHHRSHHKHSDEPGDIHSPVQRGFW
                     WSHVGWILASTHDETDARRIGDFTKYPELVWLNRFHLLPPIAFAVMTYVIGGWWALVW
                     CYFVSTTLLWHGTFTINSLCHLWGSRRYATTDDSRNNLFLALITMGEGWHNNHHHYQR
                     AVNQGFYWWELDLSYYLLRICSWAGLVWALHTRRPHDRRAASSTISLRARDR"
     gene            complement(8998..9714)
                     /locus_tag="VF516_25655"
     CDS             complement(8998..9714)
                     /locus_tag="VF516_25655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111152.1"
                     /translation="MLTVTDLHAGYGLSEVLVGASLEVKSGTIVALIGANGAGKTTTM
                     RAISGTLAPTRGEVRLGGTRVDGLGAARIARLGLAHAPEGRKVFGPLSTEDNLLLGAF
                     RWLPHLFGFHRQARQALERVYDLFPRLADRRAQPAGTLSGGEQQMLASGRALMARPKI
                     MLLDEPSMGLAPVIVQEVFRTIRRLKAEGMTMLLVEQFASAALGVADHVYVMERGRVA
                     VSGTPDELRRDPRVLAAYLG"
BASE COUNT         1310 a         3524 c         3401 g         1652 t
ORIGIN      
        1 gctgagcgcg ggccccgcgg cagctcagcc cgaggcgggg tccggcgatc cactcggtcc
       61 ggtgcgctgc tacgcgaccg cgacgcggga ttcgcttgcg ccgatggagc aggcccggca
      121 gctgtgcatc ggcgccgtcg acgaatctcc tgcgcgatgc ttcgcccggg ccgccgcccg
      181 ggagtttgcc gacgcccagg cgatccagct gtgcgccggc acgacctcgc tcgcgcctgc
      241 gatgtgcgcg gaccggctca agccgagcgg gctcgacgac ggcaccatcg tggcctactg
      301 cgcggcgctg ccctggccgc tgttcccgct cgccggcccg ggcgcaccgg cctgcgtgct
      361 cggcgcccgg ggccgcaccg ggctgtcgga caccgacgcc gtgcggctgt gcggcggctc
      421 gacgtcgacc gcgccggtcg actgcttcgc ctggggcgcg gccaacacgt atctatcgga
      481 ccacgacctc gtcgagctct gcctgccggt cgggctgtac ccctacccgc cgtgaccgcg
      541 atgccatcac ggccgcggct cgccgagctg ctgctcgagc agcttcgacg cgacgttcat
      601 gaagtcctcc tcggtcagga tcccgaccag cgcgccgtcc tgcagcaccg gcaggcagcc
      661 gtagcggtag cgccgcatca gcgcgatcgc gtcgagcgtg cgagcctcgg gggtgacggt
      721 gagcacgtcg gtccgcatga tcgccgacac cggcgtgtcg tgcagcgagc cgccgccggc
      781 gaggaaccgc agcacggcgc gataggtgac caggccgacc aggcggtggt ccttgtcctc
      841 gaccgggacg tggcggatcc gctcccagct catcaggttg acgaccatct cgatcggatc
      901 ggaggcgtgg acggtgaaca ggtcggtcgt catgtagtga tcgacccgct ggtagctggt
      961 ctcgccggcc gcggattcgt cgagccgcgc ccgctcccac tccgcgaccg ggcgcccggt
     1021 ctgctggcgc tgcagggtcg ccgcgaccag cgcgctggag cgctcgcccg aggtcgcctt
     1081 gtccttgagc gcgttccacg acgacaagag ccagcgcgcc ccggtgcgcc cggtgcggac
     1141 ccgggcgtcg atcaccgaca ggtagcgctt gatatcgtcc tcgacgatgc cctgccggcg
     1201 cagcccggcc tcggcgagcg gcagcaggcg atcgagcacg aggtgcgacg cggcgatatc
     1261 ctcgccgtcg agccagatga agtggacgcc gaggccctcg cgcgccgcgg cgtagaagtt
     1321 cgcgccggcc tggtcgaagt cgatccgccg ggtcacgtcc tcctcgcgcg cgccgagctc
     1381 ggccatcacg ccgaaccaca gcgcgccgtt ggcgatctcg tcgagcacgg tcgggccggc
     1441 gggcatcacc cggttctcga tccgcaggtg ggccttgccg tccatcacgc cgtagcacgc
     1501 gcggttccag cggtagaccg tgccgttgtg caggcgcagc gccttgagca gcggggtctc
     1561 gccgcgcgcc agcttggcca tcggatcctc gtcgagatcg gtgccgacca gggtgcggaa
     1621 ccgcgcgatg tcctcctggt agatctcgag caccgactcg cggacccagc gcgtgccgaa
     1681 gctgacccgg gcctcggtct cgcgcaggtg gtgcttgtgg ctgcgggtgt cgaccgcctg
     1741 gcggaacagt gcgatccggg tctcggccca cagccggcgg ccgaacagca gcggcgagtt
     1801 cgcggcgacc gcgaccatcg gcccggcgag cacctgggcg aggttgtaca tccgcgggaa
     1861 ctcgaccggg gtgacctgga ggtggacctg gaagctcgcg ttgcaggcct cgagcatcac
     1921 cgagtcgtgg cggacgatca gctcgtcggt gccgttgatc gagaactcga acgtgccgcc
     1981 gcgcaggtcc gccatcgcct ggttgagcgc gcggtagcgc gggctgggca ccatgccctc
     2041 gagcccgagg tcggacttgc gcagggtcgg caagatgccc atcagcaccg gggcgagccc
     2101 gagctcggcg gccgcggcgc gcgccttgcc gaccagatgg tcgagctgct tctccatccg
     2161 cgacagcccg tcgccggtga acagctgcgg atcggcgttg gcctcgagct ggaacaggcc
     2221 gagctcggtc gtgtagtgcg gatcgcggag cttgtcgagc atctgcagcg acccgcgcgc
     2281 tggcccccag tggcgatcga tcaggaacat ctcctgctcg gcgccgatcc ggcgcggccc
     2341 ggtctcgaac cggccctcgc gcagcatgcg atcgagcgcg cgcaggtcgg cgagcatcgc
     2401 gtgcatgaac tcgcggcgct tgtgctcgtc ctgctcggtg tcgacgtcct tgtgggcgcg
     2461 cgtggccttg gcgatcggtg gccctgcgtc ggtcgatttg tcgctcggct ggtcgctcat
     2521 ggcggggctc gcgacggtgt cgccgctgcg ctccacgata gcccgcccgg gtccgtcctg
     2581 tcgcgctcgc acaccccgat cagtaccggc cctccagggt gagcgccacg gcgctcgggc
     2641 cggcgcccgg cgtgagtcgc aacgcggatc gcgacagcgc ctcgtgcgct ggcggcgctg
     2701 cggtggcgta gcctctcgat cccgcggtag gatgcgcagc tcatggggcg gtttcgctat
     2761 tatctgccca gcgcggtcac catcgtcggc atcgcgttcg ccgcgatcgc ggtgcagcgc
     2821 gcggtcgagg gccagtaccg caccgcggcg tggtgggcga tgtactgcct gctcaccgat
     2881 aacatcgatg gcgcgatcgc gcggcggctg cgcgcgtcgt cgccgttcgg tgctcagctc
     2941 gactcgttcg ccgacttctt ctcgttcggc gtggccccgg ccacggtgtt ctatgcgttc
     3001 ttcgccagca cgcccggcgc cggctggagc gacccggtcc accgcggcgt gctgtcgctg
     3061 ctcgtactcc tgctcatcac ctgcgtggcg atccggctgg cgcgcttcaa cgtgacccac
     3121 agcgtggccg gcgcgaagcg gttcttcgtc ggctggccga cgacgtacat cggcggaatg
     3181 ctcgctgcgc tgttcacgct ggcgctcaag tacggcgacc cggcgtgggc cgcggacgac
     3241 ccgggcggcg accagctgcg gctgttcggc gatgtgcggc tcgacgggat gatgcggctg
     3301 ctgccgtggc tgctcctgcc cgccagcctg gtgatgatat cgaagttgcg gatgccccgc
     3361 gcggcgctga ccgggcggcg ctgggtcgat atcctgctgg cggtcacgat ggtggtcggc
     3421 tatggcctgg ggctggcgcg tcggctgccc gagtacctgg tcgccggcgg cgtggtgtac
     3481 atggctatct cgctgcgtta ccacctgatc tcgaggggcg cgtccccgtt gccgccgttg
     3541 tttcccgcgg acgatcgcta atcggtgtcc cgcgccgcgg ccggcggtgc ggagacgatg
     3601 gcaatcgcag ccatcgcggc ggccccggga actcgttgca tctgcctccg gcaagcgtaa
     3661 gatggacgat ctagggtggt gagcgccaag cgtatcgtgg ccgagttgct gggcagggcg
     3721 ggagtcggga ccgacggcgc ccagccctgg gacctccacg tgctcgacga gcgcctctat
     3781 ggccgggtca tcgcgcaggg gtcgctgggg ttcggcgaca gctacgtcga cggctggtgg
     3841 gagtgtgcgc gcatcgacga gctgatccat cgcctgctgt cgcacgacct cgaggccgag
     3901 ctcggcacgg actggggggt gcggctgcag ctgctcaagg ctcggctgct caacctgcag
     3961 gcgccgtcgc gcgcgccggc ggtcgccgcg cactacgacc tcggcaacga gttcttcgag
     4021 cgcgtgctcg gcaagaccgt gatgtactcg tgcgcgtact ggcggcgcgc cggcgatctc
     4081 gatgccgcgc agctcgacaa gatcgacctg gtcgcgcgca agctcgaggt cgcggccggc
     4141 gatcacgtcc tcgatctcgg ctgtggttgg ggcgcgatcg ccgaacatct cgcgaggaat
     4201 tgcggctgcc gggtcacggc agtgacgatc tctccggccc aggccgagta tgtgcgccgg
     4261 cgctgcaccg gcctgccggt cgaggtcatc gaggccgatt atcgcgacgc gcgggttgct
     4321 tccggtgggc cctacgacaa gctggcgtgc gtcggaatga tggaacatgt cggtcgcaag
     4381 aactaccgca acttcttctc gctgtgcgcc cggttggtca agcccggcgg tctgtgcctg
     4441 gtccacacga tcgggaccag cgtcaccacc gatgtcacgg atccgtggat cgagcgccgg
     4501 atcttcccgg gcagcgcact gccgtcggtg gccgacgtgg cgcacgccgc ggagggccag
     4561 ctgactctcg aggactggca taacttcggc gccgactacg accgcacgct gctcgcgtgg
     4621 cacgagaact tcgccgccta cgcggcgtcc ggcgagctcc agcgcggcga gcgcttcaac
     4681 cggacctggc agtactatct cctgtcgttc gccggctgct tccggacccg ccggaccatc
     4741 cagctctggc agatggtgtt ctcgcgcgat gggcgaaagg gcggctatgt ctcggttcgc
     4801 tgacctcacc gcggcggcgg ccgatcgggc ggcgacgctc ggcggcatgc tcgaggcgct
     4861 ctacctgtcg cggttcgaga tcccgccgat cgacgcggcc gacgcggccc gcggcgtcca
     4921 gcgcatcgag ctcgagcacg ccacggtgcg gctgcggatc gccggcgacg gtgcgcaccc
     4981 catcgtcatc gtgcccgatc cgcccaacac cctcgagcac tacgacgagc tgatcgatct
     5041 gctcctggcg cgcggcggcg tccgggtgat ctgcttcgag gtgcccggct tcgggctgtc
     5101 gttcccgcgt tcgctcacct tcagcttcag cgcggccgag tacaccgacg tgatcgtcga
     5161 gctgatcgat cgcctcgcgc tgcgcgacgt gacgctggtg atctcgtgca tcggcggcta
     5221 cgtcggcctc atggcggccc gcctgcggcc cgatctgatc ggccggctgg tgctgtgcca
     5281 gaccccggcc gtgcaccaca tgatcgcgtg ggcccgacgg ttcgacaagc tcggcctgct
     5341 cggcctgccg ctggtcggcc aggcgatcat gtggctgctc aaggacccgg cgacccggtt
     5401 ctggtactcg gcggcgctgc cgatcggcgc cgacgttgcg cgctaccagg caccggcgct
     5461 cgccgcccag cggatcggcg gcccgtacgc gctggcctcg gggatccagg cgatgcgccg
     5521 tctcgatgtg cgcgagctcg ctggcgtgac ccagccggtc acgctcgcgt ggggccacgg
     5581 cgatcgcacc cacgccagaa cggatcctcg ctcgctgctc gccgtggtgc cgcacgcccg
     5641 gttcgtatcc ttcgagcgtt gcggccactt ccccgacctc gaggacccgc agcgcttcac
     5701 cgagctcgtg ttcgacagcc tggaggccgg ggggtgaccg gacgacccga tcgaccctac
     5761 gatccggcgc gcgatcatgc gctcctgatc cagctcgcgc aacggctgcg cgccgcgggc
     5821 tgcttcgcgc gctgcgatca ccggtacgcc cgccggctcg cgttcctggt cccgctgttt
     5881 gccaccggat tcgccgcgct gctcgccgtg ccgcgcggcc cgatctgggc gctcgcgatc
     5941 gccgggaccg cgttcgtctc ggtccagctc ggcatcgtgt cgcacgacgc cggccatcgc
     6001 gcggtccacc gccgccgcgg ctggaacgac ctgtggggcc acgtcgggat gacgttcgtg
     6061 tgcggcctca gcttcaccca ctggcggatc gtccacgaca cacatcaccg ccactcccag
     6121 gacgagctcc gcgaccccga catgcagtac gcggcggtgt tctcggtcca cgggcgcggc
     6181 gccgccgaga agcgcgggct cgcccgcgtg atgcgcccgc tccagcactt ctatttctgg
     6241 ccgctcggcg cgctgtactc gtggtcgctg cgctgggaca gcatcgcgcg gctgttccgt
     6301 gaccccgacc gcacccgcgt cgatcgctgg gtcctgcccg gccactacgc gctgtggctc
     6361 gtcggtccgg cgctgatcgt cggcccggcg gcggcggttg tcagctatgt gctggtctcg
     6421 acgctcatcg gcctgtacct ggtgctgatc ttcgcgccca accacatggg catgccgagc
     6481 cggcgtgccg gcgagcgctg gacctatctc gagcagcaga tcatcacgtc gcgtgatctg
     6541 acggttggcc gcctgctcgc ggtcgtcctc ggcgggctcg agcaccagat cgagcatcac
     6601 ctgtttccca acatcggcca gccgcggctg cgcgccgcgc gcgcgatcgt caccgcgttc
     6661 tgtgtcgagc acggcctgtc gcaccacgag gtcggttttt tcgccgccca ccgcgcggtg
     6721 ctcggccagc tcgcctgcat ggcccggttc cccgtgcctg ccccggaggg gctgacatga
     6781 agcgtcgccc cgccgatccg ctgtatgtca agctgtcgcg cgaggtcgcg cgcgccggat
     6841 acttcaccag ggaccggtac ggctacctgt ggcgcggcgt gctgctgatc gcgctgttcg
     6901 tcatcaccta tgtttcgttg ttattcatcc gcaatctcgc attcgtatcc ttcgcgctcg
     6961 tgctgatggc ccaggtcgtc acccagatcg cctacctgtc ccacgacgcc ggtcacaacg
     7021 cgatcacccg cgaccggcgc tggatccaga tcgtcggcca tttcggcatg acgttcttga
     7081 ccggcttctc gttcagctgg tggatgcact cccacgataa ccaccacggc aagctcaacg
     7141 aacgcgatca cgatctcgcc atgaagtact cgacggtgct ggcggtccac gaggacgcgg
     7201 tgcgccagaa gcgcggggca cgccgcgccg tcctgcgctg gcagggccat tacgtgtggc
     7261 tgctcatgcc attctaccat tttgcgatga tgttcgacgg cttcctgtgg atcgcccgga
     7321 acccgcggac gacgcggatc gatcagctcg ttctgcccgg ctacatcgcg ctgtggttca
     7381 tcgtgccggt gccgttcatc ggctggaagc tcgccgcgat ccactggctg gccgagtcga
     7441 tgatcgcctc gatctatctg gtcctcacct tcatcgtcca ccacgtcggc aagaaggtga
     7501 tcctgccgtc cgacgagccc gcgatgacgc tgctgcgcca gcagctcgag accacccgca
     7561 ccctggcgac ctggcggatc ttcgactggt actactccgg cctcaactac cacatcgagc
     7621 accacctgtt cccctgggtg ccgcactggc gctatcgcaa gatgaggttc ctggtccagg
     7681 acttctgccg ccgcgagggc atcgcgtatc gcgagcaggg cttctggtcg gcggtggtcg
     7741 aggtgttcgt ccacctccgc cgcgtcggcc gctgcgcgga cctcgatgca cccgcggcgc
     7801 tgtccacact gcgcagcgcc cacgagcccc agaccgaggg gtgatgcgcc ctgtcgtcca
     7861 tcgtccagcc cacgcgacga ggccctgcac accgggcgcc tcgacggcgc accgcgcccg
     7921 gacggacaag gtcgcctggc ggggtgtcat gccgttcatc ggcgtgcatg ccgccgcgtt
     7981 cgtcggcgtc gccgtcctcg gatggtcgtg gcaagggctc ctgcttgccg tgctgttcta
     8041 cttcgtgcgc atgttcggca tcacggcggg cttccaccgc tactttgcgc accgctcgtt
     8101 caagaccagc cgcaccttcc agctcctgct cgccatcctg ggcaccacga gcacacagaa
     8161 gggtgtgctc tggtgggcgg cccaccatcg ctcccaccac aagcactcgg acgagcccgg
     8221 ggacattcat tcgcccgtgc agcgcggctt ctggtggtcg cacgtcggct ggattctcgc
     8281 gagcacccac gacgaaacgg acgccaggcg gatcggcgac ttcacgaaat accccgagct
     8341 ggtctggctg aatcggttcc acttgcttcc gcccatcgcg ttcgccgtga tgacctacgt
     8401 gatcggaggg tggtgggccc tcgtgtggtg ctacttcgta tcgacgacgc tcctgtggca
     8461 cggcacgttc acgatcaact cgctctgtca cctgtggggt agccggcggt acgcgacgac
     8521 cgacgacagc aggaacaacc tgtttctcgc gctcatcacg atgggcgagg gctggcacaa
     8581 caatcaccat cattaccagc gcgccgtgaa ccagggcttt tactggtggg aactcgacct
     8641 gagctactac ctcctccgga tctgttcctg ggccgggctg gtctgggctc ttcacacgcg
     8701 gcgcccgcac gaccgtcgcg ccgcgagctc gacgatctcc ttgcgcgcgc gcgacaggtg
     8761 agtgcggacg gtcgctgccg ggagaccctg cagccgcgag atctcgtcgc ccgacaggcc
     8821 ggcgacgtcg aacagcagga ggctgtctcg ccatcgcggg ttcagctgac ggatcagctg
     8881 gcacagaacc agctcgcgct gcttgcacgc gagctgctcg tccgggccat ccaaggacga
     8941 ggttcgcgcg gcggcgctgc gcatcgcgcg gcgcgcgagc acgccgcttc ccggcgatca
     9001 gcccaggtag gccgcgagca cgcgcggatc gcggcgcagc tcgtcgggcg tacccgacac
     9061 ggcgacgcgc ccgcgctcca tgacatagac gtgatccgcc acgccgagcg ccgcgctggc
     9121 gaactgctcg acgagcagca tcgtcatccc ctcggccttg agccggcgga tcgtgcggaa
     9181 cacctcctgc acgatcaccg gcgcgaggcc catcgacggc tcgtcgagca gcatgatctt
     9241 cggccgcgcc atcagcgccc ggccgctcgc cagcatctgc tgctcgccac ccgacagcgt
     9301 gccggcgggc tgtgcacgcc gatcggcgag ccgcgggaac aggtcgtaga cccgctcgag
     9361 ggcctgcctg gcctgccggt gaaagccgaa caggtgcggc agccagcgga acgcgccgag
     9421 caggaggttg tcctcggtgg acagcggccc gaacaccttg cggccctcgg gcgcgtgggc
     9481 gaggccgagg cgggcgatcc gcgccgcgcc gaggccgtcg acgcgcgtac cgccgagccg
     9541 aacctcgccg cgggtcggcg cgagcgtgcc ggagatcgcg cgcatggtgg tggtcttgcc
     9601 ggcgccgttg gccccgatca gcgccacgat cgtgccggac ttgacctcga gcgaggcgcc
     9661 gaccagcacc tcggacaggc cgtacccggc gtggagatcg gtgacggtca gcatgcagct
     9721 cctacggggc tccggtgggg gcggatgcgg caggcgcggg cgcaggcgcc tcggatgcag
     9781 gcgctgcgtc gggctcggcg gcgccgaggt atgcggtgat caccttcgga tcgcgcttga
     9841 tctgcgcggg cggcccctcg gcgatgacct tgccaccgtc gagcacg
//
LOCUS       DASVOR010001343         2044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393123,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001343 DASVOR010000000
VERSION     DASVOR010001343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393123"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..>2044
                     /locus_tag="VF516_25660"
     CDS             43..>2044
                     /locus_tag="VF516_25660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8111153.1"
                     /translation="MSLVVLDEVTLFFADRMIFDAVSLRLGHGDRVGLIGPNGSGKTT
                     LLKVVAGEQEIDDGKLTRANGVRVGWLPQDLAIAGGRSLIDLILSSVPGRAELHGRLA
                     TAEAELEELTRTGGDEAALLDAAADIAELHDRIDHFERLFGEHEALAILAGLGFGSGD
                     ETRDIGELSGGWKMRGVLAGLLFQRPDVLLLDEPTNHLDLPSVAWFGEFLKRWNRCFV
                     LISHDREFLNEQISRVVSLELEGVRSYPGNYEKYVEQRAEEETILAGKAKNLARERER
                     LTRFVDRFRAQANKARAVQSRIKSLERMESVETYQKRGVMRFSFPPTARTVNEVVRVE
                     GLRKAYGSHVVFPGIDLTVRRGEKIGIIGVNGAGKTTLLRMLAGELPHDAGAIRIGSG
                     VEVGYYAQHHADTLDLSATVYEVVQRASPDTPPARVRSILGAFMFSGDDVDKPVKVLS
                     GGEKARVALARLLVAPGNLLLMDEPTNHLDLASSESLAASLGTFDGTLVFVSHNRSLI
                     RTLATKIWNVEDQRVEVYPGTLDEYMYSMAQRRIAVATAETAPRRDPRGAAPLAPAAP
                     AAKSRDDDKARKRREAEARQKRSVKLGPLEKAVAQLEDRIAQLEAEQKTRSAELADPS
                     VYDEAARRNRLLSEYQAASDKLEELTARWEAAMAALEAAKAEL"
BASE COUNT          319 a          748 c          703 g          274 t
ORIGIN      
        1 ggggggcgcg ccggcgctga acgtgctatg taacgcgccg tcatgagcct ggtcgtcctc
       61 gacgaagtga ccctgttctt cgccgaccgg atgatcttcg acgcggtgtc gctccggctc
      121 ggccacggcg atcgggtcgg cctgatcggc cccaacggct cgggcaagac caccctgctc
      181 aaggtggtcg ccggcgagca ggagatcgat gacggcaagc tcacccgcgc caacggcgtc
      241 cgggtcggct ggctgcccca ggacctcgcg atcgccggcg gccggtcgct gatcgacctg
      301 atcctgtcga gcgtgcccgg ccgcgccgag ctccacggcc ggctcgccac cgccgaggcc
      361 gagctcgagg agctgacccg gacgggcggc gacgaggccg cgctgctcga cgccgcggcc
      421 gacatcgccg agctgcacga ccggatcgat cacttcgagc ggctgttcgg cgagcacgag
      481 gcgctcgcga tcctcgccgg cctcgggttc ggctccggcg acgagacccg cgacatcggc
      541 gagctgtccg gcggctggaa gatgcgcggc gtgctcgcgg gcctcctgtt ccagcgcccg
      601 gacgtgctct tgctcgacga gccgaccaac cacctcgacc tgccgtcggt cgcgtggttc
      661 ggcgagttct tgaagcgctg gaaccgctgc ttcgtgctga tcagccacga ccgcgagttc
      721 ctcaacgagc agatctcgcg ggtcgtgtcg ctcgagctcg agggcgtgcg cagctacccg
      781 ggcaactacg agaagtacgt cgagcagcgc gccgaggagg agaccatcct cgccggcaag
      841 gccaagaacc tggcgcgcga gcgcgagcgg ctgacccggt tcgtcgaccg gttccgcgcc
      901 caggcgaaca aggcgcgcgc ggtgcagtcg cggatcaagt cgctcgagcg catggagtcg
      961 gtcgagacct accagaagcg cggcgtcatg cggttctcgt tcccgccgac cgcgcgcacg
     1021 gtcaacgagg tcgtccgggt cgagggcctg cgcaaggcct acggcagcca cgtcgtgttc
     1081 cccggcatcg acctgaccgt gcggcgcggc gagaagatcg gcatcatcgg cgtcaacggc
     1141 gccggcaaga ccacgctcct ccgcatgctc gccggcgagc tgccgcacga cgccggcgcg
     1201 atccggatcg gcagcggcgt cgaggtcggc tactacgcgc agcaccacgc cgacaccctc
     1261 gacctgtccg ccacggtgta cgaggtcgtc cagcgcgcgt cgcccgacac cccgccggcg
     1321 cgggtccgct cgatcctcgg cgcgttcatg ttctcgggcg acgacgtcga caagccggtc
     1381 aaggtgctgt cgggcggcga gaaggcccgg gtcgcgctcg cccgcctgct ggtcgctccc
     1441 ggcaacctgc tgctgatgga cgagccgacc aaccacctcg atctcgcgtc gtccgagagc
     1501 ctcgccgcgt cgctcggcac gttcgacggc acgctggtgt tcgtcagcca caaccgcagc
     1561 ctgatccgca cgctcgccac caagatctgg aacgtcgagg accagcgcgt cgaggtctac
     1621 cccggtacgc tcgacgagta catgtactcg atggcccagc gccggatcgc ggtcgcgacc
     1681 gccgagaccg cgccccgccg cgacccgcgc ggtgccgcac ccctcgcccc cgccgctccc
     1741 gccgccaagt cgcgcgacga cgacaaggcg cgcaagcggc gcgaggccga ggcccgtcaa
     1801 aagcgcagcg tcaagctcgg accgctcgag aaggcggtcg cccagctcga ggaccggatc
     1861 gctcagctgg aggccgagca gaagacgcgg tcggccgagc tcgccgaccc cagcgtctac
     1921 gacgaggcag cgcgccgtaa ccggctatta tccgaatacc aggcggcttc tgacaagctc
     1981 gaagagctga cggcgcgctg ggaggcagcg atggccgcgc tggaagccgc caaggccgag
     2041 cttg
//
LOCUS       DASVOR010001344         1635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_481524,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001344 DASVOR010000000
VERSION     DASVOR010001344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1635
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481524"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF516_25665"
     CDS             <1..346
                     /locus_tag="VF516_25665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111154.1"
                     /translation="VPARIDATAGAPIPVAITAVAPSGVALHIHQALPAGVQVDASSL
                     EAQVAADAIRRFTASDGALDLDVPPLAPGTTFALTYRAIATLAGTLHTGASRIEAGAD
                     RFDVPPATWVIK"
     gene            complement(339..1418)
                     /locus_tag="VF516_25670"
     CDS             complement(339..1418)
                     /locus_tag="VF516_25670"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8111155.1"
                     /translation="MNESSSTSIELVSVAAYARQVRPLLPENAFAPARARALWIPVHY
                     AIIALLGWALASGRVPWPAWPVVSLVIGCSMAGVTFVAHEALHGGVVRGRVLIRVIGW
                     LGFLPFCISPQLWMAWHNRVHHNHCGKPGVDPDMYPTLDEYRAHRGSRVMADYFGLGR
                     RRIGGIATLLFGLTGQSVQVMFRARRIGMLTRRLHRRAIVEAVMAVAVWAAVAALVGG
                     TAFLFVYVLPLIVANTIVMTFIMTNHNLSPLSTVNDPLVNSLSVTLPRVLEWLTLDFG
                     FHVEHHLFPAMSHRRGRVVREVLRAQFPERYQSMPLGAAIRRLHHTARVYRDHTTLID
                     PNTGETWPTLMPRPRPQPPAPTPAT"
BASE COUNT          241 a          607 c          585 g          202 t
ORIGIN      
        1 cgtgcccgcg cggatcgacg ccaccgccgg cgcgccgatc ccggtcgcga tcaccgcggt
       61 cgcgccctcg ggcgtcgcgc tccacatcca ccaggcgctg cccgccggcg tgcaggtcga
      121 cgcatccagc ctcgaggccc aggtcgcggc cgacgcgatc cggcggttca ccgcgtccga
      181 cggcgcgctc gacctcgacg tcccgccgct ggcgcccggc acgacgttcg cgctgaccta
      241 ccgcgcgatc gccacgctcg ccggcacgct gcacaccggc gcgtcgcgga tcgaggccgg
      301 cgccgaccgc ttcgacgtcc cgccggcgac ctgggtgatc aagtagccgg cgtgggcgcc
      361 ggtggctgcg gccgcggccg cggcatcagc gtcggccagg tctcgccggt gttcggatcg
      421 atcagcgtcg tgtggtcgcg gtagacccgc gcggtgtggt gcaggcggcg gatcgccgcg
      481 ccgagcggca tcgactggta gcgctcgggg aactgcgcgc gcagcacctc gcgcacgacc
      541 cggccccggc ggtggctcat cgccgggaac aggtggtgct cgacgtggaa cccgaagtcg
      601 agcgtcagcc actccagcac gcgcggcagg gtcacgctca gcgagttgac cagcgggtcg
      661 ttgaccgtcg acagcggcga caggttgtgg ttggtcatga tgaacgtcat gacgatggtg
      721 ttggcgacga tcagcggcag cacgtagacg aacaggaacg cggttccgcc gaccagcgct
      781 gccaccgcag cccacaccgc gaccgccatc accgcctcga cgatcgcgcg ccggtgcagc
      841 cggcgcgtca gcatgccgat gcggcgcgcc cggaacatca cctggacgct ctgcccggtg
      901 aggccgaaca gcagggtggc gatcccgccg atccggcgcc ggccgagccc gaagtagtcg
      961 gccatgaccc gcgagccgcg gtgggcccgg tactcgtcga gcgtcgggta catgtccgga
     1021 tcgacgccgg gcttgccgca gtggttgtgg tggacccggt tgtgccacgc catccacagc
     1081 tgcggcgaga tgcagaacgg caggaagccg agccagccga tcacccggat cagcacgcgg
     1141 ccgcggacca cgccgccgtg cagcgcctcg tgggcgacga acgtgacgcc cgccatgctg
     1201 cagccgatca cgagcgacac caccggccac gccggccagg gcacgcggcc ggacgccagc
     1261 gcccagccga gcagcgcgat gatcgcgtag tggaccggga tccacagcgc ccgcgcacgc
     1321 gccggcgcga acgcgttctc ggggaggagc ggccgaacct gccgcgcata cgccgccacg
     1381 gacacgagct ctatcgaggt ggagctggac tcgttcatcg agacatccat ccgtatgacc
     1441 gctgggtacc gcggcggccg gtcattgtca ccacgcgtcc tgcaacggtt tggtcagaac
     1501 gcatgtcgcg cggcgtcgtg acgcgtcacc cgggtgcgag gttctcgccg tcgggcagcg
     1561 ccgcgagcgc ccgccgcgca gcgcgcacgc tccccgcgcg ggagcgcagc gcgtgccacg
     1621 ggtcaccccg gcgat
//
LOCUS       DASVOR010001345         8421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43254,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001345 DASVOR010000000
VERSION     DASVOR010001345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8421
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43254"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..294)
                     /locus_tag="VF516_25675"
     CDS             complement(<1..294)
                     /locus_tag="VF516_25675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111156.1"
                     /translation="MRKLIMIAILGGGGLLAMEHLTHGDDLGDADRLTVAVYGDAPYG
                     TSPTDSAQFLATPAFIQSINADPDVRLVLHVGDIHSGKQFCTEAYDRAVFNLWS"
     gene            540..1046
                     /locus_tag="VF516_25680"
     CDS             540..1046
                     /locus_tag="VF516_25680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111157.1"
                     /translation="MAAAVLVGCSDHRVQELDVPGFHALLTHQDTAVQVEKILIEPRD
                     RDGAEYVVFLKNTPVRRVVHAGFPGTFVDEIYDAGIPYAVKPKTRPSVEKELEMDATT
                     LRRRLRDPGVVAQIASIRAEPLGQGAARYVVMIKGNPVPRIVYAEYPGAIVTAIQAAK
                     VPYTVASE"
     gene            1347..2666
                     /locus_tag="VF516_25685"
     CDS             1347..2666
                     /locus_tag="VF516_25685"
                     /inference="COORDINATES: protein motif:HMM:NF019236.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8111158.1"
                     /translation="MIRLCFWTACILTLGLTCSSFAATYYVATTGSDANTGSSTAPWS
                     TLQHAVDAIAPGDTILVRSGSYAGCRIRNSGTPSAPKMLAPDVGATVVVNTPGPQNSH
                     SSLIEIENGSGSEVTDWIIDGLELANSPHHGIDIRITNRITVRNCHVHHSAPSATGTG
                     IFLAFSAHPTLENNESAFNTEHGIYDSNSADHPTIRGNHLHHNSKAGLHMNGDLSARC
                     PCGTTVRDGIISFALVENNVIYENGTAGASGINADGVDDSIFRNNLLYDNHASGVSLF
                     GADGAHGSSHNKVYNNTIVQAADGRWVVNIPGKGNKGNPVGNVLKNNILYTERADKGT
                     IAAYSTAAGVLDSDYNVVVDRFSTDGGTSITSTLAQWKALGYDAHSLVSSAATLFVDP
                     AGKMYQLNTGSPAIDAGVNLAPDVVMDIDGVARPQRTAYDIGCYEMP"
     gene            complement(3108..3689)
                     /locus_tag="VF516_25690"
     CDS             complement(3108..3689)
                     /locus_tag="VF516_25690"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEX8111159.1"
                     /translation="MSKIRDEIVQANRRYADSFGDKAKLALPPARGFAVLTCMDARID
                     PAKLAGLAEGDAHVIRNAGGRASDDAIRSLVISHKLLGTNEWFVIHHTNCGMELFTDE
                     IMRNLLAQSLATASYDGARWSDPGRGGGSHAGDFIDWLTIKDQAASVVIDVERIRQHP
                     LVPGSIPIHGFIYEVTTGRLVEVPRATEAGRPR"
     gene            3891..4547
                     /locus_tag="VF516_25695"
     CDS             3891..4547
                     /locus_tag="VF516_25695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111160.1"
                     /translation="MSPEQVIDRSAFRWLKPVAAVIAALVVAYILWSAWDHRAVMAWI
                     YELRPVPFFLVMALLPAVGFPFTPFFMFAGASFGIVVGLIGSLIALAVNLVVCFAIAR
                     HMRGPLQSLLRRFKYELPDFGARQQGSLRFAFAVKAAPGIPGFVKHYGLGLAGVPFAQ
                     YFVVALLMSGIYAAASVIVGDSLFDHDIGRTVVTVAILAGVTALVVWLRRRRARRRAG
                     "
     gene            complement(4557..5699)
                     /locus_tag="VF516_25700"
     CDS             complement(4557..5699)
                     /locus_tag="VF516_25700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111161.1"
                     /translation="MAGTGTFDDFHLGEGGLLRRLEVKCHLTKLPWQILGVVLVTWVP
                     IVVLGVLERSGDPRLSDLGMHVRLLVAAPVLLYLDLQFPKVCRRTILQLVEQEFIPEA
                     EQPRLVRTLRAAARLADSRLPELVLAVVGIGLSVASFLRLVPFGGTAWRTVLTPAQLW
                     YAVIALPLFEFLLLRSLWRWAIWVRVLVGLARIDLDLEPTHPDCRGGIAFLRMPSIEY
                     CATLLFVVASVVCATWHGWFASGATVVSFAPLLLLFATIGTVVAFGPLLMFTPRLGRV
                     RRAGLVEIGGLAAKLGRQFRQRWIQGRALVTEADASADAQALAAIGSSYRDSVHRLRT
                     LLFDRRDLLVLIVATAIPVVPVMIARIPREDWVKMAKLIGGSFVPI"
     gene            complement(5844..8366)
                     /locus_tag="VF516_25705"
     CDS             complement(5844..8366)
                     /locus_tag="VF516_25705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX8111162.1"
                     /translation="MRAALIAGGLAMLAACHDLPDLGTCGNAIVEQAHGEACDDGGES
                     ATCTASCELKCTASAVIASYVHVGLGGKAEFCPDASYRCGHDGVCRAPSGAFEKLAAL
                     VPFDVATPPVISDVDNDGVVDLVGTSMTKISVGFGSTGTPLGNVVIQDAPFSDAPYAI
                     FDLPANRPDADRPGTLMAIPTEGVALLRSDGERFTPQLDLPFQIPRSIGEVTGLVVRD
                     PDPAIGDVPIVVQSTSATPSITVARVPVGLPPLSMQMLPPCVGSGNQRWRTVDVKAAP
                     DRQSFVVVTQRDDAAQPAAQPWHVCRYTYAGTSWALADVELLPPTPSSIALANLDGDS
                     CLELVVHSDGAPGLALLDAAGAGCGFASTAAPLPIAGAGAALLGAGPIVPGGLDELVL
                     ADGVYRACIGPADCGASPTGTFVRVAGPTFPTRWSAAAITDLNGDDVLDVVAGRVRAT
                     AANPTPTGEAAVDIVRGGAAPNVYTATTSEPVTSLVAGDFDGDRLGDVAMIESAPPGG
                     PGDRITVLFGTREATAGTPVAMSPFGGRLRIGRASEIHWLPTTRSSDGIDDLVVIKAD
                     MDPSVAPTAGLLVGDAARLMTTPHFPPTATTTAAVQPLGAVAAGSFGSDDLELLALTG
                     PQAQLYNVMANSWAPPVDLGRQLDQPVAALRGGPGPTRAAARGDGNNVVVFSVRGAVT
                     TCQVTAAGTPIELRGVDIDGDGIDELAVLFDDGKGARTLQLFRTTGCPLQPLAIDALT
                     GCVDVVNAGKGLVAICRVPDASASPMMDDPAARELRAIASTGGTFVTTRRTGIDGDAR
                     FVTAGDFDGDGVLDVAVGVHRGSGVGVQLLRQCPAHDTRACP"
BASE COUNT         1351 a         2945 c         2876 g         1249 t
ORIGIN      
        1 gctccacaga ttgaacacgg cccgatcgta cgcctcggtg cagaactgct tgcccgagtg
       61 gatgtcgccg acgtgcaaca cgagcctgac gtcgggatcg gcgttgatcg actggatgaa
      121 cgccggcgtc gcgaggaact gcgcgctgtc ggtcggcgat gtaccgtacg gcgcatcgcc
      181 gtacacggcg acggtcagcc ggtcggcgtc accgaggtcg tcaccgtgtg tcaggtgttc
      241 catcgcgagc aagccaccgc cgccgaggat ggcgatcatg atcagctttc gcatcgagct
      301 ctccttggga tgcccgcacg ctaaagggcg tgctgctacg cccgatgctc ggatgtgtga
      361 cacctcgatg acggaatcgt cgcggccatg gttcggcgat ccgcgggcgg ccagagcgcg
      421 ccgccgcgac cgagcgtgac gccgccgcgg ctcgggacgt cgaaaaacga ccgcggcatg
      481 atagcgtgca agcgctggtg ctgcgccgac gtctgcacat acgaagctgg tcgtggctcg
      541 tggccgccgc ggtcctcgtg ggctgctcgg accaccgcgt ccaggagctc gacgtcccag
      601 ggttccacgc gctgctgact caccaggata ccgcggtcca ggtcgagaag atcctcatcg
      661 agccgcgcga tcgtgatggc gccgagtacg tggtgttcct caagaacacc cccgtgcggc
      721 gcgtcgttca tgccgggttt ccgggaacgt tcgtggacga gatctacgac gccggcattc
      781 cgtacgcggt gaagccgaag acccggccga gcgtcgagaa agagctggag atggacgcga
      841 cgacgctccg tcgccgcctg agggatcccg gggtggtcgc ccagatcgcg agcatccgcg
      901 ccgaaccgct gggacagggc gctgccaggt acgtcgtgat gatcaagggc aatcccgtcc
      961 cgcgaatcgt ctacgccgag tatcccggtg cgatcgtcac ggcgatccag gcggccaagg
     1021 ttccgtacac ggtcgcgtcc gagtgactgg tgcgagcgcg cgccccgtcg ccttgaagcg
     1081 accaggacgc agcaccgtac cgctcgcact gcctggtatt gccacgagtc gcacctcgta
     1141 atgcccgtta ctgtcatttc tccggaggca gctggcgatc gaatggccca ggtccctacg
     1201 caccgaactt ctcgcagttt accgcatcgg tattgccgaa cacgaactat gctgcctaca
     1261 aacgcatccc agccctatag gggagtcgtc taccccgacg agaactttgt aggatgcccg
     1321 accctcgaac ggggaaagga catatgatga ttcgactctg cttctggact gcgtgtattc
     1381 tgactctggg tctgacctgc tcctcttttg ccgccacgta ctatgtcgca accaccggta
     1441 gcgacgcgaa caccggcagc tccaccgcac cgtggagcac gctccagcac gccgtggatg
     1501 ccatcgcacc cggggacacg atcctggtgc gctctggcag ctacgccggc tgccgcatcc
     1561 gcaactcggg gacaccatca gcccccaaga tgctcgcgcc cgacgtgggc gccacggtcg
     1621 tcgtcaacac gcccggacca cagaacagtc actcgagcct gatcgagatc gagaacgggt
     1681 ccggctcgga ggtcacggac tggatcatcg acgggctcga gctggccaac tcgccgcacc
     1741 acggcatcga catccgcatc accaaccgca tcacggtccg gaactgccac gtgcatcaca
     1801 gcgcgccttc ggcgaccggc accgggatct tcctcgcctt ctccgctcat ccgacgctcg
     1861 agaacaacga gtccgccttc aacaccgagc acgggatcta cgacagcaac agcgccgacc
     1921 acccgaccat ccgcggcaac cacctgcacc acaacagcaa agccggcctc cacatgaacg
     1981 gcgacctcag cgcgcgctgt ccctgcggga ccacggtgcg ggacgggatc atctctttcg
     2041 cgctggtgga gaacaacgtc atctacgaga acggaaccgc cggcgcctcc ggcatcaatg
     2101 ccgatggcgt ggatgacagc atcttccgca acaacctgct ctacgacaat cacgcctccg
     2161 gtgtgtcgtt gtttggcgcc gacggtgcgc atggatccag ccacaacaag gtctacaaca
     2221 acaccatcgt tcaggccgcc gacggacgct gggtggtgaa cattcccggg aagggcaaca
     2281 agggcaaccc cgtcggcaac gtgctgaaga acaacatcct gtacacggag cgcgcagaca
     2341 aggggaccat cgccgcctac agcacggcgg ccggagtcct cgacagcgac tacaacgtgg
     2401 tcgtggaccg gttcagcacc gacggcggca cttcaatcac gagcacgctc gcgcagtgga
     2461 aggcactcgg atacgacgcg cactccctcg tgtccagcgc cgccaccttg ttcgtggacc
     2521 cggcgggcaa gatgtaccag ctcaacaccg ggtcgcctgc catcgacgcg ggagtgaacc
     2581 tcgcccccga cgtggtgatg gacatcgacg gcgtggcgcg cccccagcgt acggcctacg
     2641 acatcggctg ctacgagatg ccgtagtccc gaactggacg atcgtcatcg cccgcgggca
     2701 gactggtcga gcaggtgctg ctccagcggc ggggaagacg gcgtcgaccg gctggcacat
     2761 gtgtcacggt ccggtccccg tgccagcgcc tccggtgcca cgtgccggcc gcgcaggtgc
     2821 tcgatgtcgc gacgcaatcg ccgggcatcc gggatgcagc ccggtccctc caggagacac
     2881 cccgtgaacg catcatctct gggattcgtg gtggcgttgc tggtcacctt ggttgcgctg
     2941 cgccgccggc tgcgcagaca gctccgccgc aacgagctgt cgatgacgta catcccgtgg
     3001 tgacgacagc gtcagctcgt ccggctcccc gggctcgtcc gcctcgtccg cggcgccagc
     3061 gcgactggta accgacatcc aacggatctc ggcgccgcca cgtcaggtca gcgcggccgg
     3121 ccggcctcgg tcgcccgcgg gacctcgacc agccggccgg tggtgacctc gtagatgaag
     3181 ccgtggatcg ggatcgagcc cgggaccagc gggtgctggc ggatccgctc gacgtcgatc
     3241 acgacgctgg cggcctggtc cttgatcgtc agccagtcga tgaagtcacc ggcgtgcgag
     3301 ccgccacctc tgcccggatc gctccaccgg gcgccgtcgt agctcgcggt cgccaggctc
     3361 tgcgcgagca ggttgcgcat gatctcgtcg gtgaacagct ccatgccgca gttggtgtga
     3421 tggatgacga accactcgtt ggtgccgagc agcttgtgcg agatcaccag cgagcggatc
     3481 gcgtcgtcgc tcgcgcgccc gccggcgttg cggatcacgt gggcgtcgcc ctcggcgagc
     3541 ccggcgagct tggctggatc gatccgggca tccatgcagg tgaggacagc gaacccgcgc
     3601 gcgggcggca gcgcgagctt ggccttgtct ccaaagctgt cggcgtaacg gcgattggcc
     3661 tgaacgattt cgtcgcggat cttgctcatg gcagtctccc gggcagtcgt gcccggccag
     3721 atgttgctcc ggcgcgtcat gccccggggc gagcgagctg ccgcaaatcg cggcagctca
     3781 tcgtgatgcc gaccacgaag tcgacgcacg cgcgggtgcc cgccatgccc acggcgcacg
     3841 ccgcccccgg cactcgcgat gcaactcgcg cgatcatgag ctcgacgacg gtgagcccgg
     3901 aacaggtgat cgaccgctcc gccttccgct ggctgaagcc ggtggccgcg gtgatcgccg
     3961 cgctggtcgt tgcctacatc ctgtggtcgg cgtgggacca ccgcgcggtg atggcgtgga
     4021 tctacgagct ccgtcccgtg ccgttcttcc tcgtgatggc gctgttgccg gccgtcggct
     4081 tcccgttcac cccgttcttc atgttcgcgg gcgcctcgtt cgggatcgtc gtgggcttga
     4141 tcggctcgct gatcgcgctc gcggtcaacc tggtggtgtg cttcgcgatc gctcgccaca
     4201 tgcgtgggcc gctccagtcg ttgctccgcc ggttcaagta cgagctcccg gacttcggcg
     4261 cgcgtcagca aggaagcctg cgcttcgcgt tcgccgtgaa ggcggcgccc ggtatccccg
     4321 ggttcgtcaa gcactacggc ctcgggctcg ccggcgtgcc gtttgcccag tacttcgtcg
     4381 tcgcgctgct gatgtcgggg atctacgcag cggcgtccgt gatcgtcgga gattcgctgt
     4441 tcgaccacga catcggccgc acggtcgtga ccgtcgcgat cctcgccggc gtgacggccc
     4501 tcgtcgtgtg gctgcgccgg cgccgcgcga gacggcgcgc gggctagcca ccacagctag
     4561 atcggcacga agctgccgcc gatcagcttg gccatcttca cccagtcctc gcgcgggatg
     4621 cgcgcgatca tcaccggcac gaccgggatc gcggtggcga cgatcaggac gagcaagtcg
     4681 cgccggtcga acagcagcgt ccgcagccga tggacgctgt cgcggtagct cgacccgatc
     4741 gcggcgagcg cttgcgcgtc ggcgctcgcg tcggcctcgg tcacgagcgc tcgtccctgg
     4801 atccagcgct gccggaactg ccgcccgagc ttcgctgcca gaccgccgat ctcgacgagc
     4861 cctgcgcggc gcacgcgccc gaggcgcgga gtgaacatca ggagcgggcc gaacgcgacc
     4921 acggtcccga tggtcgcgaa cagcagcagc aggggcgcga agctgacgac cgtcgcgccg
     4981 gacgcgaacc agccgtgcca ggtcgcgcac accacgctcg ccaccacgaa caggagcgtc
     5041 gcgcagtact cgatcgaggg catgcggaga aacgcgatgc cgccgcggca gtcgggatgc
     5101 gtcggctcga gatcgagatc gatccgcgcg agaccgacga gcacgcgcac ccagatcgcc
     5161 caccgccaca gcgagcgcag cagcaagaac tcgaacagcg gcagcgcgat gaccgcatac
     5221 cacagctgcg ccggcgtcag gaccgtccgc cacgcggtgc cgccgaacgg gaccagccgc
     5281 aggaacgacg cgacgctgag gccgatgccc accaccgcga gcacgagctc gggcagccgc
     5341 gagtcggcga ggcgcgccgc cgcccgcagc gttcgcacga gcctgggctg ctcggcctcg
     5401 gggatgaatt cctgctccac gagctggagg atcgtgcggc ggcagacctt cgggaattgc
     5461 agatcgaggt agagcaggac gggtgcggcc accagcagtc ggacgtgcat gcccagatcg
     5521 gacagccgcg gatcgcccga ccgctcgagc acgccgagca cgacgatcgg cacccaggtg
     5581 acgaggacga ctcccaggat ctgccacggc aacttcgtga ggtgacactt gacctccagg
     5641 cgccggagca accctccttc gccgaggtga aagtcgtcga acgttccggt tccggccatg
     5701 gccgcgcgat cgagtcgtgc agaggccatg ccctcgcctt ctcggcaagg cgcggagcag
     5761 cgaaattccg tgcgcggtgc gaagcgcgca gcgatcacgg cgcacacctc acaccgccgg
     5821 ccggcgtccg cggaacctgg aggctacggg caggcgcggg tgtcgtgcgc cgggcactgc
     5881 cgcagcagct ggacgccgac gcccgagccg cggtgcacgc cgacggcgac gtcgagcaca
     5941 ccgtcaccgt cgaaatcgcc ggccgtcacg aagcgggcgt cgccgtcgat gccggtgcgc
     6001 cgcgtcgtga cgaacgtgcc gcccgtgctc gcgatcgcgc gcagctcccg cgcggccggg
     6061 tcgtccatca tcggggaagc gctcgcgtcg gggacccggc agatcgcgac cagacccttg
     6121 ccggcgttca cgacgtcgac gcacccggtg agcgcatcga tggccagcgg ctggagcggg
     6181 cagcccgtgg tgcggaacag ctgcagcgtg cgcgctccct tgccgtcgtc gaacaacacg
     6241 gcgagctcgt cgatcccgtc gccgtcgatg tcgaccccgc gcagctcgat cggcgtgccg
     6301 gccgcggtga cctggcacgt ggtgacggcg ccgcgcacgg agaacacgac gacgttgttg
     6361 ccatcgcccc gggcggcggc gcgcgtgggt cccggaccgc cgcgcagcgc ggccacgggc
     6421 tgatcgagct gcctcccgag gtcgaccggc ggcgcccagc tgttcgccat cacgttgtag
     6481 agctgcgcct gcgggccggt gagcgccagg agctcgaggt cgtcgctgcc gaagctgccg
     6541 gcggccacgg cgccgagcgg ttgcacggcg gcggtcgtgg ttgccgtcgg cggaaagtgc
     6601 ggcgtggtca tcaggcgagc ggcgtcgccg accaggagcc cggcggtcgg ggcgacgctg
     6661 ggatccatgt cggccttgat cacgaccagg tcgtcgatgc catcgctgct gcgcgtcgtc
     6721 gggagccagt ggatctcgct ggcgcggccg atccggagcc ggcccccgaa cgggctcatc
     6781 gcgaccggcg tgccggcggt cgcttcgcgg gtaccgaaca ggacggtgat gcgatcgccg
     6841 ggtccgccgg gtggggccga ctcgatcatc gccacgtcgc ccaggcgatc gccgtcgaag
     6901 tcgcccgcga ccaggctcgt gaccggctcg gacgtggtcg cggtatacac gttgggggcc
     6961 gcgccgccgc ggacgatgtc gacggcggct tcgccggtgg gcgtggggtt ggcggcggtc
     7021 gcgcggacgc gcccggcgac gacatcgagc acgtcatccc cgttgaggtc ggtgatcgcc
     7081 gcggcgctcc accgggtcgg gaaggtcggc ccggcgacgc gcacgaacgt gccggtgggg
     7141 ctggccccgc aatcggccgg gccgatgcac gctcgataca cgccatctgc gagcacgagc
     7201 tcgtcgaggc cgccggggac gatcgggccg gccccgagca gcgccgctcc ggccccggcg
     7261 atcgggagcg gcgcagccgt cgaggcgaac ccacatccgg cgccggccgc gtcgagcagg
     7321 gcgagcccgg gtgcgccatc gctgtgcacg acgagctcga ggcacgagtc cccgtcgaga
     7381 ttggcgagcg cgatcgacga cggggtcggt ggcaggagct cgacgtcggc gagcgcccag
     7441 ctggtgccgg cgtacgtgta gcggcacacg tgccatggct gcgccgccgg ctgcgccgca
     7501 tcgtcgcgct gggtgacgac cacgaacgat tgccgatcgg gcgcggcctt gacgtccacc
     7561 gtgcgccagc gctggttccc gctccccacg cacggtggca gcatctgcat cgacagcgga
     7621 ggcaacccga ccgggacccg cgcgacggtg atgctcggag tcgcgctcgt gctctgcacc
     7681 acgatgggga cgtccccgat cgccggatcg ggatcccgca cgacgaggcc cgtgacctcg
     7741 ccgatgctcc tggggatctg gaacggcaga tcgagctggg gcgtgaagcg ctcgccgtcg
     7801 gagcgcagca gcgcgacccc ctcggtgggg atcgccatca gggtgccggg ccggtccgcg
     7861 tcggggcggt tcgcgggtag atcgaagatc gcatacggcg catccgagaa cggcgcatcc
     7921 tggatcacga cattgccgag cggcgtcccg gtgctgccga acccgacgga gatcttggtc
     7981 atgctggtcc cgaccagatc gaccacgccg tcgttgtcga catcgctgat caccggcggc
     8041 gtcgcgacgt cgaacggcac cagcgccgcg agcttctcga acgcgcccga gggcgcgcgg
     8101 cacacgccat cgtggccgca gcggtagctc gcgtcggggc aaaattcggc cttgccgccg
     8161 aggcccacat ggacgtacga cgcgatcacc gccgaggcgg tgcacttcag ctcgcagctc
     8221 gccgtgcacg tggcgctctc gccgccatcg tcgcaggcct cgccgtgggc ctgctcgacg
     8281 atggcgttgc cgcaggtgcc gaggtcggga aggtcatggc aggcggccag catcgcgagc
     8341 ccgcctgcga tcagggccgc gcgcatcaga atcctcctgc gtagccgatg ccgtggccga
     8401 ggccaccgcc gaacgtcatc g
//
LOCUS       DASVOR010001346         2059 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_159666,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001346 DASVOR010000000
VERSION     DASVOR010001346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159666"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..1672)
                     /locus_tag="VF516_25710"
     CDS             complement(245..1672)
                     /locus_tag="VF516_25710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111163.1"
                     /translation="MRGFLTVSSRVWFSGLFSGLVLALSLAQGACGGCNDNNSVGHLP
                     DAPPAPDAAPDAIPPDAVSLVVIDHGVPVAGVPVYFLNPDGSVAKATVTGGNGTASAV
                     VTTGGSATAIRPFMTQAPAGKFAPDELRTFLGVKPGDHLVLSRAATDSVTVTIDAVAP
                     DGGDFSTGYQVLTTCGGGVNSISPGGSGDGSGSPNPGGTLELQNCHGAADIAVVASER
                     ALARAGGNAIATPQGLYHANATLPPGGNVLVSLRDDTYADLTDVTFTYMNAPGVPITV
                     LHSPVLPNGYLGPYSTTVDTGSTDATISEAAVPATMEVVDSTLQPNNLHEIVDWGPYG
                     PTYALDVGATLLREVTGGPTFDLATGKISWTEATTGATPSLTIAGVLVRRPDPLAKWH
                     WEIVAPYSASGIVLPHLPTTLLDWAPAATDEITLDPLQNVQVTGGYDAVRSHILDIHD
                     ETGSTGYVIGNAGRAVIETSVIQPL"
BASE COUNT          290 a          742 c          734 g          293 t
ORIGIN      
        1 tcgatctcga tcgcctccag cacgccgtgc gcgaccgccg agcgcaccgt cgcgccgtgc
       61 caggcgccgg gggcgtcgag gtcgtcgagg tactgcgccc ggccggtgac cttggcgacg
      121 ccatcgacgc gcggcagcga gcggccgacg ggtggcacgg caggtcgagc agcttccatc
      181 ccgggatcta acctcaggcc gcgcgcggag taaacgcacc ggcggcaacc gccgcgcgcc
      241 gccgctacag cggctgaatg accgacgtct cgatgacggc gcggccggcg ttgccgatca
      301 cgtacccggt cgagccggtc tcgtcgtgga tgtcgaggat gtgcgagcgg accgcgtcgt
      361 agccgccggt cacctggacg ttctggagcg gatcgagggt gatctcgtcg gtcgcggcgg
      421 gcgcccagtc gagcagggtg gtcggcaggt gcgggagcac gatcccgctg gccgagtatg
      481 gcgccacgat ctcccagtgc cacttcgcga gcgggtccgg gcgccgcacc aggacccccg
      541 cgatcgtcag gctcggcgtc gcgccggtgg tcgcctcggt ccacgagatc ttgccggtcg
      601 ccagatcgaa cgtcggccct ccggtcacct cgcggagcag ggtggcgccc acgtcgagcg
      661 cataggtcgg cccgtacggc ccccagtcga cgatctcgtg caggttgttc ggctgcagcg
      721 tcgagtcgac gacctccatc gtcgccggca cggccgcctc ggagatcgtc gcgtccgtgc
      781 tcccggtatc cacggtggtc gagtacggcc cgaggtagcc gttgggcaac accggcgagt
      841 gcagcacggt gatcggtaca ccgggcgcgt tcatgtacgt gaacgtcacg tcggtcaggt
      901 ccgcgtacgt gtcgtcgcgc aggctcacca ggacgttccc gccgggcggc aacgtcgcgt
      961 tggcgtggta caggccctgc ggagttgcga tcgcgttgcc gcccgcccgg gccagcgcgc
     1021 gctcgctcgc cacgaccgcg atgtcggccg cgccgtggca gttctgcagc tccagcgtgc
     1081 cgccgggatt cgggctgccc gagccgtcgc cgctgccgcc cggcgagatc gagttgacac
     1141 cgccgccgca ggtcgtgagc acctggtagc cggtgctgaa gtcgccgccg tccggggcga
     1201 ccgcgtcgat cgtgaccgtc accgagtcgg tcgccgcccg gctgagcacc aggtgatcgc
     1261 ctggcttgac ccccaggaac gtgcgcagct cgtcgggggc gaacttgccc gccggagcct
     1321 gggtcatgaa cggccggatc gcggtcgccg atccgcccgt ggtcaccacc gcgctcgcgg
     1381 tcccgttccc gccggtgacg gtggccttcg cgaccgagcc gtcgggattg aggaagtaga
     1441 ccggcacgcc ggcgaccggc acgccgtggt cgatcaccac cagcgacacc gcatcgggag
     1501 gtatcgcgtc gggggcggca tcgggcgccg gcggcgcgtc gggcaggtgc cccacggagt
     1561 tgttgtcgtt gcacccgccg cacgcgccct gcgccaggct cagcgccaga acgaggcccg
     1621 agaacagccc cgagaaccac acgcgcgagg acacggtcag aaagccacgc atgagggcag
     1681 ggatgatacc caaacgcggc ggcgaacatc taccatcccg tccgtgcgta cccggcagct
     1741 cggaccctac accgtcactg caatcggcag cggcgacctc tgcttgccga cctctgccag
     1801 ccgcggggtc ctggcgcgtg acgtcgagca cgccttgcac gaagcgatcg gattcggggt
     1861 cgcgctgttc gacgtcgccg ccgaccccga cagcgagaag ctcgccggcg gcgccgtccg
     1921 caccggccgc gctgcgcgct catggccccg tcacgacgac cgcgcggccg gcgtcgccgg
     1981 tcatgtaccg ggtcaggtcg aaccagctgt gtcgatcgaa gatgtgcgac cggaccgcgt
     2041 cgtagccgcc ggtcacctt
//
LOCUS       DASVOR010001347         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_189392,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001347 DASVOR010000000
VERSION     DASVOR010001347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189392"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1029
                     /locus_tag="VF516_25715"
     CDS             <1..1029
                     /locus_tag="VF516_25715"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HEX8111164.1"
                     /translation="PAEVDPLIATIGRELPPLRGVVHAAGVLDDGVLLNQTAERFAAV
                     MAPKVAGSWNLHQATRDLPLDFFVLFSGAGTLLGSPGQGNYAAANAFLDALAQARRAE
                     GLPGQSIAWGPWSEIGLAARVERGGRLALHGVASLSPPDGVEALGRVMQDGAAQIAVL
                     PLDVRQCLQSYPTLAGSPLFEVLARGAPEHPVHAAADASFLRMLASLAPAERHGALEH
                     HLRDHAARVLGLPPARIPVQEPLGNLGFDSLMTLELRNRIESSLGLKLSATVVWNYPT
                     IAALAEYLARKLDLAPPEPADTSAPPAAAVSAFTSAVRDLTEDEAVAALAATLGQFEH
                     GDRHDQAR"
     gene            1013..>1676
                     /locus_tag="VF516_25720"
     CDS             1013..>1676
                     /locus_tag="VF516_25720"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEX8111165.1"
                     /translation="MTKPADSNELSLVKRSLVALQEMKARLDASERQLTEPIAVIGMG
                     CRFPGGARDPGSFWRLLEAGGDAISQVPGERWDIDTFFHPEPATPGKMSSRWGGFLDQ
                     IDGFDAAFFGISPREAVRMDPQQRLLLEVAWEALEDAGQTRERLAGTRTGVFAGICST
                     DYSWLQFADPDDIDAYTGTGTSLSILAGRLAYLLDLQGPSVALDTACSSSLVAVHLAC
                     QSL"
BASE COUNT          219 a          639 c          565 g          253 t
ORIGIN      
        1 ccggccgagg tcgacccgct gatcgccacc atcggccgcg agctgccacc gctgcgcggt
       61 gtcgtccatg ccgccggtgt cctcgacgac ggcgtcctcc tcaaccagac cgccgagcgg
      121 ttcgccgccg tgatggcgcc caaggtcgcc gggagctgga acctgcacca ggcgacgcgg
      181 gatctcccgc tcgacttctt cgtgctgttc tcgggcgccg gcaccctgct gggctcgccg
      241 ggacagggca actacgccgc ggcgaatgcg ttcctcgacg cgctcgcaca ggcccggcgc
      301 gccgagggac tccccgggca gagcatcgcc tggggcccct ggtccgagat cgggctcgcc
      361 gcccgcgtcg agcgcggtgg acgactcgcg ctgcacggcg tcgcgagctt gtcacccccc
      421 gacggtgtcg aggcgctggg ccgcgtgatg caggacggcg cggcgcagat cgcggtcctg
      481 ccgctggacg tccgccagtg cttgcagtcg tacccgacgc tcgctggatc gccgctgttc
      541 gaggtcctgg cgcgtggcgc gccggagcac ccggtacacg cggccgccga cgcatcgttc
      601 ctccgcatgc tggcgtcgct cgctcccgcg gagcgccacg gcgcgctcga gcaccacctg
      661 cgcgatcacg ccgcccgggt gctcggactt ccgccggcgc ggatccccgt gcaagagccg
      721 ctcggcaacc tcgggttcga ctcgctcatg acgctcgagc tccgtaaccg catcgagtcg
      781 agcctcgggc tcaagctgtc ggccaccgtg gtctggaact acccgaccat cgccgcactg
      841 gccgagtacc tcgcgcgcaa gctggatctg gcgcccccgg agccagccga cacatccgca
      901 ccgcccgccg ccgccgtctc cgcgttcacc tcggcggtgc gcgacctcac cgaggacgag
      961 gccgtcgctg ccctcgccgc aacgctcggc cagttcgagc atggagatcg tcatgaccaa
     1021 gcccgctgat tcgaatgagc tctcgctggt caagcgcagc ctggtcgcgc tccaggagat
     1081 gaaagcccgc ctcgacgcgt cggagcggca gctcaccgag ccgatcgcgg tcatcggcat
     1141 ggggtgccgg ttcccgggtg gtgcccgcga tccggggtcg ttctggcgcc tgctcgaagc
     1201 cggcggtgac gcgatctccc aggtccccgg cgagcgctgg gacatcgata cgttcttcca
     1261 ccccgagccc gccacgcccg gcaagatgag cagccggtgg ggtggcttcc tcgatcagat
     1321 cgacggcttc gatgcggcgt tcttcgggat ctcgccccgc gaggcagtcc gcatggaccc
     1381 gcagcagcgg ttgttgctcg aggtggcgtg ggaggcgctc gaggacgccg gacagacccg
     1441 cgagcgcctg gccgggaccc gcaccggcgt gttcgccggc atctgctcca ccgattacag
     1501 ctggctgcag ttcgcggacc ccgacgacat cgacgcctac accgggaccg gcacgtcgct
     1561 cagcatcctc gccggccggc tggcgtacct cctcgatctg cagggcccga gcgtggcgct
     1621 cgacaccgcg tgctcgtcct cgctcgtggc ggtgcacctc gcctgccaga gcctgc
//
LOCUS       DASVOR010001348         4194 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_276057,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001348 DASVOR010000000
VERSION     DASVOR010001348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4194
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276057"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2390
                     /gene="fdnG"
                     /locus_tag="VF516_25725"
     CDS             <1..2390
                     /gene="fdnG"
                     /locus_tag="VF516_25725"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HEX8111166.1"
                     /translation="VGFKWFIEARKTRGAKLVVVDPRFTRTAAVADYYAPIRPGTDIA
                     FLLGIIRYAIETKRFHEDYVKLHTNASYIIGDKYEFKVEDGLFSGFDPEKKEYDKSNW
                     AYAADDASKAYKVDPTLSDERCVFQLLKKHVERYTPEMVERICGTPKAAFLKVAEMVT
                     STGNAEKVGTITYALGWTQHSTGVQIIRAAAMLQLLLGNVGRPGGGVNAFRGHSNIQG
                     ATDMAGTFEILPGYLKTPTGPMQSLAQYNEMTTPTTLNKVAWPSMNYWQNTPKFIVSL
                     LKATWGDKATADNEFGYSWLPKIDGNYSWLFIFDDMYRGTSKRAGGDEPGPEGFISFG
                     MNPVGTGPNSKKMVAALSKLKWMVMVENVETETAQFWKAPAEYEPPPPGQIPTEVYLL
                     PASSFAEKDGTFTNSARWLQWKWRATDPPGQAKSDQEILARIVVAVRELYKKDGGKFP
                     DPVLNLSWSYANPANPDLSDVLKEINGKALADLPDPKDPTKVLRPAGQQVNGFGELRD
                     DGTTMCGNWLHSGVFTEAGNNAQRRSTADPTGLGMFHQWAFSWPANRRVMYNRASADA
                     NGAPWDPKRVGIKWNGQAWVGDVPDIKPDSPPGQFGAFIMNAEGVGKLFSASLNDGPF
                     PEHYEAIEAPIDNPLHPKTTSNPVSKRFSSDKDKYGKREEFPIVCTTYRLTEHFHYWT
                     QHHAAGKLNELQPGFFIEVPEALAQQKGITNGSQIKLRSARGEIQGVAMVTKRLPTLK
                     IDGKDVWQIGFPIHWGFAGDKGHTGPLANMLTPSAMDANTWTPEYKAFLVQLEKA"
     gene            2404..3966
                     /gene="fdxH"
                     /locus_tag="VF516_25730"
     CDS             2404..3966
                     /gene="fdxH"
                     /locus_tag="VF516_25730"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit beta"
                     /protein_id="HEX8111167.1"
                     /translation="MSNAPQLEIRQRSAALRGESTGIRSLPVVSKFIDTTTCIGCKAC
                     EVACQEWNDLQLVSTKQTGTYQTMPTLDANFWNLIKFREEERNGALAWLMRKDQCMHC
                     ADPGCLKACPAPGAIVQYENGIVDVNPDACIGCGLCATGCPFDVPRFHAETGKMAKCT
                     LCVDRVSVGIEPACVKACPTGCLQFGTKEDMLALGQQRVATLKANGNAEAGLYDPPGV
                     GGTTVVTVLGHADHPEWYGLPKDPHVPASAWISRKLLRPIGLLAMAGTLGAVLGHFVK
                     FGPSQPAKKELDVQPTSSGPEGKVIGDHIVRHRLSTRVIHWTTAIGFFGALLSGMPIW
                     TPIFGWMASLFGGLSVCRWLHPWFGVAFVAATLAMFVLWFKDMRMQREDWGWLGPKAL
                     AYMRHTGEDPDVGKYNGGQKLFFLLITVLAVVLVVTGLVLWFPRELARPLPDLAWVLH
                     DGGFILFAVAIVGHIYLGTAALPGTFRSMTRGTVTKAWAKLHHPRWYRDVTGEQGSGA
                     GSAGAGAHDVQK"
     gene            3950..>4194
                     /locus_tag="VF516_25735"
     CDS             3950..>4194
                     /locus_tag="VF516_25735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111168.1"
                     /translation="MTSRSSWLHRDASVADALERRAARAALLAKDSITAAPLEFAAGL
                     YRAQAKVATALAARGTPSGSLERDLDGFLRELDVIAR"
BASE COUNT          732 a         1477 c         1381 g          604 t
ORIGIN      
        1 ccgtaggctt caagtggttc atcgaggcgc gcaagacgcg cggcgccaag ctggtcgtcg
       61 tcgatccgcg gttcacgcga accgccgcgg tcgccgacta ctacgcaccg atccggccgg
      121 gaaccgacat cgcgttcctg ctcgggatca tccggtacgc gatcgagacc aagcgctttc
      181 acgaggacta cgtcaagctc cacaccaacg cctcgtacat catcggggac aagtacgagt
      241 tcaaggtcga ggacggcctg ttctcgggct tcgatccgga gaagaaggag tacgacaaga
      301 gcaactgggc gtacgcggct gatgacgcga gcaaggcgta caaggtcgat ccgacgctga
      361 gcgacgagcg ctgcgtgttc cagctgctca agaagcacgt cgagcgctac acgccggaga
      421 tggtcgagcg gatctgcggc acgcccaagg cggcgttcct caaggtcgcc gagatggtga
      481 cctcgaccgg caacgccgag aaggtcggca ccatcaccta cgcgctgggc tggacccagc
      541 actcgaccgg cgtccagatc atccgcgccg ccgcgatgct gcagctcctg ctcggcaacg
      601 tcggccggcc gggcggcggc gtcaacgcgt tccgcggcca ctccaacatc cagggtgcca
      661 ccgacatggc cggcaccttc gagatcctgc ccggctatct gaagacgccg accgggccga
      721 tgcagagcct cgcgcagtac aacgagatga ccacgccgac cacgctgaac aaggtcgcgt
      781 ggccgtcgat gaactactgg cagaacacgc cgaagttcat cgtctcgctg ctcaaggcga
      841 cgtggggcga caaggcgacc gccgacaacg agttcggcta ctcgtggctg cccaagatcg
      901 acggcaacta ctcgtggctg ttcatcttcg acgacatgta ccgcggcacg tccaagcgcg
      961 ccggcggcga cgagcccggc cccgagggct tcatcagctt cggcatgaac ccggtcggca
     1021 ccggccccaa ctccaagaag atggtggccg cgctgtccaa gctgaagtgg atggtcatgg
     1081 tcgagaacgt cgagaccgag accgcccagt tctggaaggc cccggccgag tacgagccac
     1141 cgccgcccgg tcagatcccg accgaggtct acctcttgcc ggcgtcgtcg ttcgccgaga
     1201 aggacggtac gttcaccaac tcggcgcgct ggctgcaatg gaagtggcgg gcgaccgatc
     1261 cgccggggca ggcgaagtcc gatcaggaga tcctcgcccg catcgtcgtg gcggtgcgcg
     1321 agctctacaa gaaggacggc ggcaagttcc ccgacccggt gctcaacctg tcgtggtcgt
     1381 acgccaatcc ggccaacccc gatctgagcg acgtgctcaa ggagatcaac ggcaaggcgc
     1441 tcgccgatct gcccgatccc aaggatccga ccaaggtgct tcgccccgcc ggccagcagg
     1501 tcaacgggtt cggggagctg cgcgacgacg gcacgacgat gtgcggcaac tggctgcact
     1561 ccggcgtgtt caccgaggcc ggcaacaacg cgcagcgccg cagcacggcg gacccgaccg
     1621 gcctgggcat gttccatcag tgggcgttct cgtggccggc caatcgccgc gtcatgtaca
     1681 accgcgcgtc cgcggacgcc aacggcgcgc cgtgggaccc caagcgggtc ggcatcaagt
     1741 ggaacggcca ggcctgggtc ggcgacgtgc ccgacatcaa gccggactca ccgccgggcc
     1801 agttcggcgc gttcatcatg aacgccgagg gcgtcggcaa gctgttctcg gcctcgctca
     1861 acgacggccc gttccccgag cactacgagg cgatcgaggc gccgatcgac aacccgctgc
     1921 atcccaagac cacgtccaac ccggtctcca agcggttctc ctccgacaag gacaagtacg
     1981 gcaagcggga ggaattcccg atcgtctgca ccacctaccg gctgaccgag cacttccact
     2041 actggaccca gcaccacgcc gccggcaagc tcaacgagct ccagcccggg ttcttcatcg
     2101 aggtgcccga ggcgctggcc cagcagaagg ggatcaccaa cggcagccag atcaagctgc
     2161 gctcggcgcg cggcgagatc cagggcgtcg cgatggtcac caagcggttg cccacgctca
     2221 agatcgacgg caaggacgtc tggcagatcg ggtttccgat ccactggggc ttcgccggcg
     2281 acaagggcca caccgggccg ctcgccaaca tgctgacgcc gtccgcgatg gacgccaaca
     2341 cctggacgcc cgagtacaag gcgttcctgg tccaactcga gaaggcctga ggaggaggga
     2401 cccatgagca acgctcctca gctggagatc cggcagcgct cggcggcgct gcgcggcgag
     2461 agcaccggca tccgcagcct cccggtggtg tccaagttca tcgacaccac gacctgcatc
     2521 ggctgcaagg cctgcgaggt cgcgtgccag gagtggaacg acctgcagct ggtgtcgacc
     2581 aagcagaccg gcacgtacca gacgatgccg acgctcgacg ccaacttctg gaacctgatc
     2641 aagttccgcg aggaagagcg caacggcgcg ctggcctggc tgatgcgcaa ggaccagtgc
     2701 atgcactgcg ccgatccggg ctgcctcaag gcctgcccgg cgcccggcgc gatcgtgcag
     2761 tacgagaacg gcatcgtcga cgtcaacccg gacgcgtgca tcggctgcgg cctgtgcgcc
     2821 accggctgtc cgttcgacgt gcccaggttc cacgccgaga ccggcaagat ggccaagtgc
     2881 acgctgtgcg tcgaccgcgt cagcgtcggc atcgagcccg cgtgcgtcaa ggcgtgcccg
     2941 accgggtgcc tgcagttcgg caccaaggag gacatgctcg cgctcggcca gcagcgcgtc
     3001 gcgacgctca aggccaacgg caacgccgag gccgggctct acgatccgcc cggcgtcggc
     3061 ggcaccaccg tcgtcaccgt gctcggccac gccgaccacc ccgagtggta cggcctgccc
     3121 aaagatccgc acgtcccggc ctcggcgtgg atcagccgca agctgctccg gccgatcggc
     3181 ctgctcgcga tggccggcac gctcggcgcg gtgctcggcc acttcgtgaa gttcggcccg
     3241 agccagcccg ccaagaagga gctcgacgtc cagccgacgt cgtccggccc cgagggcaag
     3301 gtgatcggcg accacatcgt ccgccaccgc ctgtcgacgc gcgtgatcca ctggaccacc
     3361 gcgatcgggt tcttcggcgc gctgctcagc ggcatgccga tctggacgcc gatcttcggc
     3421 tggatggcga gcctgttcgg cggcctgtcg gtgtgccgct ggctgcaccc gtggttcggc
     3481 gtcgcgttcg tcgccgccac gctggcgatg ttcgtcttgt ggttcaagga catgcgcatg
     3541 cagcgcgagg actggggctg gctcgggccc aaggcgctcg cctacatgcg acataccggc
     3601 gaggatcccg acgtcggcaa gtacaacggc ggccagaagc tgttcttcct gctgatcacc
     3661 gtgctcgccg tcgtgctggt cgtgaccggc ctggtgctgt ggttcccgcg cgagctcgcg
     3721 cgcccgctgc ccgatctcgc ctgggtgctc cacgatggcg gcttcatcct gttcgccgtc
     3781 gcgatcgtcg ggcacatcta cctcggcacc gcggcgctgc ccggcacgtt ccgctcgatg
     3841 acccgcggca cggtgaccaa ggcgtgggcc aagctgcacc acccgcgctg gtatcgcgac
     3901 gtcacgggcg agcagggcag cggcgcagga tccgccggcg ccggcgcgca tgacgtccag
     3961 aagtagctgg ctgcatcgcg acgcctcggt cgccgacgcg ctggagcggc gcgcggcccg
     4021 ggcggcgctg ctcgcgaagg actcgatcac cgccgcaccg ctggagttcg cggcggggct
     4081 gtaccgggcg caggccaagg tcgccaccgc gctcgccgcc cgcggcacgc cgagcggctc
     4141 gctcgagcgc gatctcgacg gcttcctgcg cgagctcgac gtcatcgcgc gcta
//
LOCUS       DASVOR010001349         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_72104,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001349 DASVOR010000000
VERSION     DASVOR010001349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72104"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1564
                     /locus_tag="VF516_25740"
     CDS             <1..1564
                     /locus_tag="VF516_25740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111169.1"
                     /translation="DKLMPTFYAEMDIQPGSIHDQIIQDKIHDDAIAKLVGGILLAIV
                     AIALTVVSLGAATPAVVAAGASIGAAGLSTYMAYDEYKQYTAEHAVADAGLADDPSVI
                     WLVLAIVGAGVDMAAATKAVRALAPAAKALHAGGELTDFTKTVEALQRSRQLDDKIAA
                     AAEKAAAARTAYAAARGELSAALGKAYSFPGPFTDPEVYRALVKMAAAKIKEGSHSLA
                     GFIAELQQARLAAKLGELSPEDLAKAKQAFDQAEALAKLVADPALLDRLLSGVGDATK
                     LERLLNVFPANELEGIVARVKNPERLVLILDHVGADSGAKMIRQWAAKGTFDKLDTFM
                     ERMSAGFPDKLAETTGVGTKSIVIDSNTAIALMKDADPARRGTMNAGEIARVKYIKSL
                     PPGTELRIGNVTVGEIDGGTLATKGLPITVLRESTEYRSLLSKLESMNLGGSKGAADR
                     ALVADVFFAKSEGGAVPTFVTGDKSIYNKLATEAGIDVENMGEKTLQDLKPNGFPVTI
                     DGRTINVVPIAQ"
     gene            1634..2041
                     /locus_tag="VF516_25745"
     CDS             1634..2041
                     /locus_tag="VF516_25745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111170.1"
                     /translation="MSTADSSTNMQTHIRDDASGTEIGVGFRLESLEVRDQDIISSGY
                     LSTGQMATDGRIRLLETWRCPACGHENWARVTISGTEVIAIEPVILDRSTLESAQYIA
                     DSCYIVASSISGIPARDLMEGKVNPVGVLFDRL"
     gene            2169..>2754
                     /locus_tag="VF516_25750"
     CDS             2169..>2754
                     /locus_tag="VF516_25750"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEX8111171.1"
                     /translation="MATARSTHSIRALLGNVALPLPVIELNGAFVSDLATGRHLVVQG
                     LAANLAAAVMARIRRRGLDAFIATSTSDEDHLYHGEPTNAGIAWYRDEKLAMGDPRLR
                     AMADPDVALAEQVVGFTLFAEHEVVRGLADELRAELAEQVQLHWFENFYCPGWWELSI
                     HDRSATKASAIAALRRSLGLEASRLVVFGDGINDL"
BASE COUNT          544 a          858 c          899 g          453 t
ORIGIN      
        1 ggacaagctc atgcccacct tctacgcgga gatggacatc cagcccgggt ccattcacga
       61 ccagatcatc caggacaaga tccacgacga tgcgatcgcc aagctcgtcg gtggcatcct
      121 gctcgcgatc gtcgccatcg cactgaccgt cgtctcgctc ggcgcggcga ccccggccgt
      181 cgtcgccgcg ggcgcgtcga tcggcgcggc gggcctcagc acgtacatgg cgtacgacga
      241 gtacaagcag tacaccgccg agcacgcggt cgccgacgcc ggccttgccg acgatccctc
      301 ggtgatctgg ctcgtcctcg cgatcgtcgg cgcaggcgtc gacatggccg cggcgaccaa
      361 ggccgtgcgc gcgctcgcac cggcggcaaa ggcgctgcac gcgggcggcg agctcaccga
      421 cttcacgaag accgtcgagg cgctgcagag gtccaggcag ctcgacgaca agatcgccgc
      481 cgccgccgag aaggcggccg ccgcccgcac agcctacgcc gcggccaggg gcgagctctc
      541 ggcggcgctc ggcaaggcct actcgttccc cgggccgttc accgatcccg aggtctacag
      601 ggcgctggtc aagatggccg ccgccaagat caaggaaggc tcccactccc tcgccggctt
      661 catcgccgag ctccagcaag cccgcctcgc ggccaagctc ggcgagctat cacccgaaga
      721 cctcgccaag gccaagcaag cgttcgacca agcggaggcg ctggcgaaac tggtagccga
      781 tcccgcgctg ctcgacaggc tgctttcagg agtaggtgac gcgacgaagc tcgagcggct
      841 cctcaacgta ttcccggcga acgagctcga agggatcgtg gcccgggtca agaaccctga
      901 gcgactggtg ctgatcctcg accacgtggg cgccgatagc ggtgccaaga tgatacgcca
      961 gtgggcagcc aagggcacct ttgataaact ggatacgttc atggaaagaa tgagcgccgg
     1021 attcccagac aagctggccg agaccacggg cgtcggtacg aaatcgatcg tcatcgactc
     1081 caacacagcg atcgctctca tgaaggacgc cgatcctgcc cgcaggggta cgatgaatgc
     1141 tggtgagatc gctcgtgtga agtacatcaa gagcctgcca ccggggacag agcttcgaat
     1201 cggcaacgtg acagtaggcg agatcgatgg aggcaccctg gccaccaagg ggttgccgat
     1261 cacggtgctg cgcgagtcga cggagtacag gtcgctgctc tccaagctag aatccatgaa
     1321 cctcggtggc agcaaaggcg ccgcggaccg cgcgctcgtc gccgatgtgt tcttcgccaa
     1381 gagcgagggc ggcgccgtac ccacgttcgt gacgggcgac aagtcgatct acaacaagct
     1441 cgctaccgag gcaggcatcg acgtggagaa catgggtgaa aagacccttc aggatctaaa
     1501 acccaatgga ttcccggtca cgatcgatgg gcgtacaatc aatgttgttc caatagctca
     1561 atgagtatag caaccaggat ccagtatgta tgactacttt gtagctgccc tcagatgtcc
     1621 caactgtaac atcgtgagca ctgctgactc atcaacgaac atgcagacgc atattcgaga
     1681 cgacgccagc gggaccgaga tcggtgttgg ctttcgtctt gagtcactcg aagtacgcga
     1741 ccaggacatc atatcatctg gatatctctc cacgggtcaa atggcaacgg atggacgaat
     1801 ccggctactc gaaacgtggc gatgcccagc gtgcggccat gagaactggg cgcgtgtgac
     1861 catctccgga accgaggtga tagcgatcga gcccgtcatc ctcgatcggt cgactctcga
     1921 gagcgcccag tacatcgccg atagttgcta catcgtcgcg tccagcattt ccggcattcc
     1981 tgcgcgcgat ctcatggagg gcaaggtcaa tcctgtgggc gtgctattcg atcggctgta
     2041 ataggaagta atgctcagta ctcccgcgca atcgcgaacc tgcgcgacaa ggattgagtc
     2101 tggtccgtga ggatacgtgc aatgtacgac tactttgctg caaaactacg gtgccaccgt
     2161 tgttcacggt ggcgacggca cggtcgacgc actcgatccg ggcgctgctc gggaacgtcg
     2221 cgctgccgct gccggtgatc gagttgaacg gggcgttcgt gtcggacctg gcgaccggcc
     2281 ggcacctggt ggtccagggc ttggcggcca acctcgctgc ggcggtgatg gcgaggatcc
     2341 ggcggcgggg cctcgatgcg ttcatcgcga cgtcgacgag cgacgaggat cacctgtacc
     2401 acggcgagcc gacgaacgcg gggatagcgt ggtatcgcga cgagaagctc gcgatgggcg
     2461 acccgcggct gcgcgcgatg gcggacccgg acgtggcgct ggccgagcag gtcgtcgggt
     2521 tcacgctgtt cgccgagcac gaggtggtgc gcgggctggc ggacgagcta cgcgcggagc
     2581 tcgcggagca ggtgcagctc cactggttcg agaacttcta ctgcccgggc tggtgggagc
     2641 tgtcgatcca cgaccgcagc gcgacgaagg cgagcgcgat cgcagcgctg cgccggtcgc
     2701 tggggctgga ggcgtcgcgg ctggtggtgt tcggcgatgg gatcaacgac ctcg
//
LOCUS       DASVOR010001350         1907 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_422282,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001350 DASVOR010000000
VERSION     DASVOR010001350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422282"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..388)
                     /gene="mraW"
                     /locus_tag="VF516_25755"
     CDS             complement(<1..388)
                     /gene="mraW"
                     /locus_tag="VF516_25755"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HEX8111172.1"
                     /translation="MVPFAHAPVLAAEVLDSLRPRDHGVYVDGTVGGAGHATLLLGRA
                     PAVRLIGIDRDPAALAASREALAAFADRVQLVHGEYGDLPRILAELDTPRLPPVDPTG
                     GSAPRPAGGAVGVPTPRLPPVDPTGAS"
     gene            558..1370
                     /locus_tag="VF516_25760"
     CDS             558..1370
                     /locus_tag="VF516_25760"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX8111173.1"
                     /translation="MLRYKETEDIDARFSEVERIVTAWRARVATANPVARTEFNNRLR
                     ISLIHHDAALEGEVLSYSEIKAAVDPSIISDPSLIPSYEEIRRFDDACNYAAEQAANK
                     KRPFKLDTIRELYSILSPDEKAKGLPFRKENPLHRLYYHDITPPEKILPSMRKLGEWI
                     DDPATKNLHVIERVATLHSRFMAIFPWAKESGRTIRIASNLLLQEGGYPIAIVHSIDR
                     QRYYEALRGDHLGLTSLYLEAVQTTAETEMRVYEEANKNPSKRRRKRDDAPG"
     gene            1390..1812
                     /gene="arfB"
                     /locus_tag="VF516_25765"
     CDS             1390..1812
                     /gene="arfB"
                     /locus_tag="VF516_25765"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEX8111174.1"
                     /translation="MQDLVVTPRVTIPGGELAIAFARSGGPGGQNVNKVASKVELRWN
                     PTTSAALTDDERAWLVHRLQRRLTGDGTLIVTSTATRDQGKNRDDATSKLALIVRAAL
                     DRPKPRRATRPSRGAKRRRLADKRHHAEIKRRRSSRDD"
BASE COUNT          351 a          636 c          657 g          263 t
ORIGIN      
        1 cagaggcccc cgtgggatcg accggaggga gacggggggt ggggaccccg acggctccgc
       61 cggcggggcg gggggcagag ccccccgtgg gatcgaccgg agggagacgg ggggtgtcga
      121 gctcggcgag gatccgaggc aggtcgccgt actcgccgtg gaccagctgg acgcggtcgg
      181 cgaaggcggc gagcgcctcc cggctcgccg cgagcgcggc ggggtcgcga tcaattccaa
      241 tcaggcggac ggccggcgcc ctgcccagaa ggagggtggc gtggccggcg ccgccgaccg
      301 ttccatcgac ataaacccca tgatctcgcg gacgtagcga atcgagcacc tcggcggcca
      361 gcaccggcgc gtgcgcaaag gggaccacgg cggctgtagc tatcatgttg atgtcgcaag
      421 gatgcagaaa tcttgcgatc tgggcgcaat cgatgtgcaa ttaggtgtag gcatactccc
      481 cacatttact tgacgatccg gatggtgtcc cgtagactcc atctttcgat tgcccaatca
      541 atccggcagg ataacacgtg ctcaggtaca aagaaaccga agacatcgat gctcgcttca
      601 gcgaggtcga gcggatcgtg acggcgtggc gtgcacgcgt cgccactgcg aatcctgtcg
      661 cgcgcaccga gttcaacaac cggctccgga tctcgctcat ccatcacgac gctgcgctcg
      721 aaggcgaggt gctgtcgtac agcgagatca aggccgcggt cgatccgagc atcatcagcg
      781 atccgtcgct gatcccgtcg tacgaggaga tccgccggtt cgacgacgcg tgcaactacg
      841 ccgcggagca ggcggcgaac aagaagaggc cgttcaagct cgacacgatc cgcgagctgt
      901 actcgatcct gtcgccggac gagaaggcca aggggctgcc gttccgcaag gagaaccccc
      961 tccaccggct gtactatcat gacatcacgc cgcccgagaa gatcctgccg tcgatgcgca
     1021 agctcggcga gtggatcgac gatccggcga ccaagaacct gcacgtgatc gagcgcgtcg
     1081 cgacgctgca ctcgcggttc atggcgatct tcccgtgggc caaggagagc ggtcgcacga
     1141 tccggatcgc gtccaatctg ttgctgcagg agggcggcta tccgatcgcc atcgtccact
     1201 cgatcgaccg ccagcgctac tacgaggcgc tgcgcggcga tcacctcggg ctgacctcgc
     1261 tgtacctcga ggcggtgcag accaccgccg agaccgagat gcgggtatac gaagaggcca
     1321 acaagaaccc gtcgaagcgc cgccgcaagc gcgacgacgc gccgggatga ccggcgccgc
     1381 agggcagcga tgcaggacct cgtcgtcaca ccgcgggtga cgatccccgg cggtgagctc
     1441 gcgatcgcgt tcgcgcgctc gggtggcccg ggcgggcaga acgtcaacaa ggtggcgagc
     1501 aaggtcgagc tgcggtggaa cccgacgacc tcggccgcgc tgaccgacga cgagcgcgcc
     1561 tggctggtcc accgcctcca gcgccggctg accggcgacg gcacgctgat cgtcacctcg
     1621 accgcgaccc gcgatcaggg gaagaaccgc gacgacgcga ccagcaagct ggcgctgatc
     1681 gtccgcgccg cgctcgaccg gcccaagccg cgccgggcga cccggccgtc gcgcggggcc
     1741 aagcgccgcc ggctcgccga caagcgccac cacgccgaga tcaagcgccg ccgctcgagt
     1801 cgcgacgact gacgcctcgg gctcgctagg tgccggccga gtagtcgctc gcgtaggtga
     1861 tctgctcggg cgtgagcttg tcgatcggga tgcccatggt cggcagc
//
LOCUS       DASVOR010001351         3848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_510567,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001351 DASVOR010000000
VERSION     DASVOR010001351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510567"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VF516_25770"
     CDS             <1..1164
                     /locus_tag="VF516_25770"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8111175.1"
                     /translation="DQERARVSADRSTAAKSADGHTPSNNQGVAATWTARLAGGHAIV
                     VGADAQRVVGTATDTLFPATVQPSTIVQRAAGGEQRFAGVFAQDAMQVTPELELAAAL
                     RLDGWQNIDASGTTTRSDGTAMTTPFADASKLQLDPRLGALYHVTHEIAVRASAYRAF
                     RAPTLNELYRPFQVGTVLTAANADLRPETLWGGELGTQLVLDRVAAQATAFWNQMSDP
                     IANVTLAMPVNGATRQRQNLGSTRILGLDLDLTWRPAEAWTVRVAHTFSDGYVRSAPA
                     QPDLVGKRLAQDPHHRSVAMVSYDNPRIATLTAEMRYLGPQFEDDQNTQPIGSVVLFD
                     ARAERALAGGFSVFVTGRNLFDRHYLVGRAGIDTEGAPRTFELGLAYHAGARR"
     gene            complement(1184..3325)
                     /locus_tag="VF516_25775"
     CDS             complement(1184..3325)
                     /locus_tag="VF516_25775"
                     /inference="COORDINATES: protein motif:HMM:NF020976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HEX8111176.1"
                     /translation="MKRREFLERTGWFVVGATLVGIPACGDDTKSKPPAPDAGGSTDP
                     VGTFSFPQGVASGDPRESSVVLWTRVVRGNDSGNVQLALEVASDPSFSTLVVDQKLTA
                     TAASDHTVRVLVTGLLFGSTYYYRFTAGQDTISGRTFTAPAAQADVQVNFAWVSCQDY
                     AAGFYGAYRQMLVDDDARADADKIFAVIHLGDMIYETRASNFQTAIDDSFNPIALANG
                     DGSKRGVAAFPSGGGTRASTNFASTLDDYRSLYRTFLSDPDFQAARARWPFISIWDDH
                     EFTDDCWQSQANYTNGMTLDEPDQIRRVAASQAWFEYVPAHLTGAGGVAGVASQAKDF
                     APPATPVTDAAFTAPNADNFVDEPNNKAAVGAITIYRSLRFGKHVELVLTDERSYRSD
                     HAIPEELAAAAGFLASRNALPKDLVNVLDLGSAFPGGAPPQIDLGGGHVITNPRASSP
                     PGTMLGKAQKQWWKDTMKGSDATWKLWGNEVTLMRLKITSLGPTSPLPADLVISADAW
                     DGYNAERTELMTFLKANAIQNVVVLTGDIHASFAGQVMDNFEAPAAAQTPVAVELVGP
                     GISSNSLFSFFYAATVSNPPLNALIAANATAAGGSKFTENFNLLLLHGTASAGAYAQN
                     LQVIPQTFDPNANAHLKYADTNSQGYGYIKVGADKVEGSIITIQRPIQTPSGTDGPGA
                     KRTATFTVASGTPNLTGPTFNTGDTKPFPLT"
     gene            complement(3441..>3848)
                     /locus_tag="VF516_25780"
     CDS             complement(3441..>3848)
                     /locus_tag="VF516_25780"
                     /inference="COORDINATES: protein motif:HMM:NF016296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8111177.1"
                     /translation="HRPVAAGAPQVPARIVARRKRALVFLRLDEVWAFEAAERLAYVH
                     TARGRFEVDLSLSAIEASIGHALLRAHRNWLVNADHVRELEGHGSDTELLVGAASGEP
                     GGELAGVRVPVARDRAQAVREALLRNAMGIRPR"
BASE COUNT          533 a         1398 c         1352 g          565 t
ORIGIN      
        1 gatcaggagc gcgcccgggt gtcggccgat cgctcgaccg ccgcgaagtc cgccgacggc
       61 cacaccccgt cgaacaacca gggcgtggcc gcgacctgga ccgcgcggct ggccggcggc
      121 cacgcgatcg tggtcggcgc cgacgcccag cgcgtggtcg ggaccgcgac cgatacgctg
      181 ttcccggcca cggtccagcc gagcacgatc gtccagcgcg ccgccggcgg cgagcagcgg
      241 ttcgccggcg tgttcgccca ggacgcgatg caggtcaccc cggagctcga gctcgccgcc
      301 gcgctgcggc tcgacggctg gcagaacatc gacgccagcg gcaccacgac gcggtccgac
      361 ggcaccgcga tgaccacccc gttcgccgac gcctccaagc tccagctcga tccgcggctc
      421 ggcgcgctgt accacgtcac gcacgagatc gcggtgcgag cctcggcgta ccgcgcgttc
      481 cgcgccccga cgctcaacga gctgtaccgg ccgttccagg tcggcaccgt gctgaccgcg
      541 gccaacgccg acctccgccc ggagaccctg tggggcggcg agctcggcac ccagctcgtg
      601 ctcgaccgcg tcgccgccca ggccaccgcg ttctggaacc agatgagcga tccgatcgcc
      661 aacgtcacgc tcgcgatgcc ggtcaacggc gcgacccgcc agcgccagaa cctcggcagc
      721 acgcggatcc tcggcctcga cctcgatctg acctggcgcc cggccgaggc gtggaccgtg
      781 cgggtcgccc acacgttcag cgacggctac gtccgcagcg cgccggccca gcccgacctg
      841 gtcggcaagc ggctcgccca ggatccccac caccgcagcg tcgcgatggt gagctacgac
      901 aacccgcgga tcgccacgct caccgccgag atgcgctacc tcggcccgca gttcgaggac
      961 gaccagaaca cgcagccgat cggctcggtc gtgctgttcg acgcccgcgc cgagcgcgcc
     1021 ctcgccggcg gcttctcggt gttcgtcacc ggccgcaacc tgttcgaccg ccactacctg
     1081 gtcggccgcg ccgggatcga caccgagggc gcaccgcgca cgttcgagct cggcctcgcc
     1141 taccacgccg gcgcgcgccg gtagcgccgc cggccccggc cgctcaggtc agcgggaacg
     1201 gcttggtatc gccggtgttg aacgtcgggc cggtcagatt tggggtcccc gaggcgaccg
     1261 tgaaggtcgc agtgcgcttg gcgccgggac cgtcggtgcc gctcggcgtc tggatcggcc
     1321 gctggatcgt gatgatcgat ccctcgacct tgtccgcgcc gaccttgatg tagccgtagc
     1381 cctgcgagtt ggtgtcggcg tacttgaggt gcgcgttggc gttcggatcg aacgtctgcg
     1441 ggatcacctg gaggttctgg gcgtacgcgc ccgcagacgc ggtgccgtgc agcagcagca
     1501 agttgaagtt ctcggtgaac ttcgagccac cggccgccgt ggcgttcgcg gcgatcagcg
     1561 cgttcagcgg cgggttcgac accgtggcag cgtagaagaa cgagaacagc gagttcgagc
     1621 tgatgccggg gccgacgagc tcgaccgcca ccggcgtctg cgcggcggcc ggcgcctcga
     1681 agttgtccat gacctgcccc gcgaacgagg cgtggatgtc gccggtcagc acgaccacgt
     1741 tctggatggc gttggccttg aggaacgtca tcagctcggt gcgctcggcg ttgtagccgt
     1801 cccacgcgtc ggcgctgatc accaggtcgg ccggcagcgg gctggtcggg ccgagcgagg
     1861 tgatcttgag ccgcatcagc gtgacctcgt tgccccacag cttccaggtc gcgtcggacc
     1921 ccttcatggt gtccttccac cactgcttct gcgccttgcc gagcatcgtg cccggcgggc
     1981 tgctggcgcg cgggttggtg atcacgtggc cgccgcccag gtcgatctgc ggcggcgcgc
     2041 cgccggggaa cgcgctgccg agatcgagca cgttgaccag atccttgggc agcgcgttgc
     2101 gcgacgcgag gaacccggcg gcggcggcga gctcctcggg aatcgcgtgg tcgctgcggt
     2161 agctgcgctc gtcagtcagc accagctcga cgtgcttgcc gaaccgcagc gagcgataga
     2221 tcgtgatcgc gccgaccgcg gccttgttgt tgggctcgtc gacgaagttg tcggcgttgg
     2281 gggcggtgaa cgccgcgtcg gtgaccggcg tggccggcgg cgcgaagtcc ttggcctggc
     2341 tggcgacccc ggcgacgccg ccggcgccgg tcaggtgcgc cggcacgtac tcgaaccacg
     2401 cctggctcgc cgcgacccgc cggatctggt ccggctcgtc gagcgtcatg ccgttggtgt
     2461 agttggcctg gctctgccag cagtcgtcgg tgaactcgtg atcgtcccag atcgagatga
     2521 acggccagcg cgcccgcgcc gcttgaaagt cgggatccga caggaaggtc ctgtacagcg
     2581 agcggtagtc gtcgagcgtg ctcgcgaagt tcgtgctcgc ccgcgtgccg ccgccgctcg
     2641 ggaacgccgc gaccccgcgc ttcgatccgt cgccgttggc cagcgcgatc gggttgaagc
     2701 tgtcgtcgat cgcggtctgg aagttgctgg cccgggtctc gtagatcatg tcgccgaggt
     2761 ggatcacggc gaagatcttg tcggcgtcgg cccgcgcgtc gtcgtcgacc agcatctggc
     2821 ggtacgcgcc gtagaagccg gccgcgtagt cctggcacga cacccaggcg aagttgacct
     2881 ggacatcagc ctgcgccgcc ggcgcggtga acgtccggcc ggagatcgtg tcctgcccgg
     2941 cggtgaaccg gtagtagtac gtcgaaccga acagcaggcc ggtgaccagc acgcgcaccg
     3001 tgtgatcgga cgccgcggtc gcggtcagct tctggtcgac gacgagggtg gagaagctgg
     3061 ggtcgctggc gacctcgagg gccagctgga cgttcccgga atcgttgccg cggaccacgc
     3121 gcgtccacag caccaccgag ctctcgcgcg gatcgcccga ggccacgccc tgcgggaagc
     3181 tgaacgtgcc cacggggtcc gtcgacccgc cggcgtcggg ggccggcggc ttcgacttcg
     3241 tgtcgtcgcc acatgccggg atgccgacca gggtcgcgcc caccacgaac cagccggtgc
     3301 gctccaagaa ttctcgtcgc ttcatcgccc tcgaagatat cggttgccgg ccgtcgccac
     3361 gagacgttcg cgcagcaatc gtgaccgaat tcgcaaccga tgacgcggcg tcgcgccccg
     3421 atcctgcccc cggatcccgc tcagcgcggc cggatcccca tggcgttgcg gagcagcgcc
     3481 tcgcgcaccg cctgcgcccg gtcgcgtgcc accgggaccc gcacgccggc gagctcgccc
     3541 cccggctcgc cgctcgccgc gccgacgagc agctcggtgt cgctgccgtg gccctcgagc
     3601 tcgcggacgt ggtcggcgtt gaccagccag ttgcgatgcg cgcgcagcag ggcgtggccg
     3661 atcgacgcct cgatcgccga cagcgacagg tcgacctcga accggccgcg cgcggtgtgg
     3721 acgtaggcga gccgctccgc ggcctcgaac gcccacacct cgtcgagccg gaggaacacc
     3781 agcgcgcgct tgcgccgggc gacgatccgc gccgggacct gcggggcgcc cgccgcgacc
     3841 ggccggtg
//
LOCUS       DASVOR010001352         3461 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_218542,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001352 DASVOR010000000
VERSION     DASVOR010001352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218542"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VF516_25785"
     CDS             <1..943
                     /locus_tag="VF516_25785"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8111178.1"
                     /translation="AARDTEARLEGLTEIDPRRRLNILKFCAAGFMSAGKPTEARARY
                     LTALTLLDQLRSSAGARAGGEARPGGEQAPGRGEGDDENASTRGGLLSHLAWIDAQVG
                     RFGSALVQFQQAVAAYTQAFGDGNPDVALFTLDVGVMQYLVGQERQAETWLEHARALI
                     DRSSPDSPIKATALDYLGFVVLRRGEAARARSLFQQALEIIERTRLTDHPALPITLLG
                     LARQQLQDAPAAGETDRRAIVAMLERAAAVGERTHADPWELAWVRLELVRALSGQHAD
                     PGRIPRLVEQIRETYAQLPELAGPERAAFQAWLAHHP"
     gene            1019..1747
                     /locus_tag="VF516_25790"
     CDS             1019..1747
                     /locus_tag="VF516_25790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111179.1"
                     /translation="MREHGDRIDRRASSAIEERVRPGLSAQLPAEDLADRAGQEPDVG
                     PGGAGRRGAHGELSEDAADRRAEVAVGGAERLGDHADERDPGPYGVAQRGLAAHRVPA
                     VEALGGQAQPGDAIGAAHEAERRRRDLDDVTAAVRVEQVGAPEVVGIGLAVVAVADDA
                     VHGVARGVLEAALDFTAAAMQIDGRSHGSSCVVDLGRPSKRRRRAACVQAPTAIRSVS
                     LSETPARMPCAYWAVVYPDPAIDS"
     gene            complement(1642..2457)
                     /locus_tag="VF516_25795"
     CDS             complement(1642..2457)
                     /locus_tag="VF516_25795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111180.1"
                     /translation="MSTPEAVLYCQGTFSAPNPTPAQMKQMMEAAKDIGNSGFSTVIL
                     GQFHVHSNGSIYYNDSPLDTVMWALETVPGLLKQAGKVQRVLVTFGPFASDFAAIQAN
                     LASFQATMAKVMAAGRIDGLDWDLEQNLSQFTTLLVQLTVWANSLGKLVTAAPYYDIP
                     FWSTVLQQTQRAGGSFAWWNLQLYGGASYPQWARSFPAAFLYAGYANTQGATPGSIQS
                     SLAALRTQFPSLTGGFLWRYESIAGSGYTTAQYAQGILAGVSDSETERIAVGA"
     gene            2664..>3461
                     /locus_tag="VF516_25800"
     CDS             2664..>3461
                     /locus_tag="VF516_25800"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX8111181.1"
                     /translation="MGHERANIARMHGYVPGKQPASSETIKLNTNENPYPPCDAVMRE
                     LAAIPAEALRRYPPPSAEGVRERAAALHGLARDQVVAVNGGDELLRLAVTTFVEPGGP
                     IGVIEPSYSLYPVLAEIHASPVVRADAEPDWSWPADLAERMNRAGVRLLLIVNPHAPS
                     GHLAPAAAVAALARAFHGVLLVDEAYVDFVDPELGHDVVGLIAQHDNLLVLRTLSKGY
                     SLAGLRFGYGLGAAPLIAPLLWKTRDSYNVDAIAQRLAAAALAARDQA"
BASE COUNT          489 a         1176 c         1314 g          482 t
ORIGIN      
        1 ggcggcccgc gacaccgagg cgcggctcga gggcctgacc gagatcgatc cgcgccggcg
       61 gttgaacatc ctgaagttct gtgcggcggg gttcatgagc gccggcaagc ccacggaggc
      121 gcgggcgcgc tatctgaccg cgctgacgct gctcgaccag ctgcgctcct ccgccggggc
      181 gcgcgcgggc ggcgaggccc gtccgggcgg cgagcaggcc cccggccgcg gtgagggcga
      241 cgacgaaaac gcctcgaccc ggggcgggct actgtcccat ctggcctgga tcgacgcgca
      301 ggtcgggcgg ttcgggagcg cgctcgtcca gttccagcag gcggtcgccg cgtacaccca
      361 ggcgttcggc gacggcaacc cggatgtcgc gctgttcacc ctcgacgtcg gggtgatgca
      421 gtacctcgtg gggcaggagc gccaggccga gacctggctg gagcacgcgc gagcgctcat
      481 cgatcggtcg tctccggata gcccgatcaa ggcgaccgcg ctcgattacc tcggcttcgt
      541 cgtgctgcgc cggggcgagg ctgcccgcgc gcgatcgctg ttccagcagg cgctcgagat
      601 catcgagcgc acccggttga ccgatcatcc ggcgctgccg atcacgctgc tgggcctcgc
      661 gcgccagcag ctgcaggacg cacccgccgc gggcgagacg gacaggcgcg ccatcgtcgc
      721 catgctcgag cgggcggccg ccgtcggcga gcggacgcat gccgatccgt gggagctggc
      781 ctgggtgcgg ctcgagctcg tgcgcgcgct gtcggggcag cacgcggatc cggggcggat
      841 cccgcgcctg gtcgagcaga tcagggagac ctacgcccag ctgcccgagc tggcaggccc
      901 ggagcgcgcc gccttccagg cctggctggc gcaccatccg tgagcggggc aggcggccgc
      961 gctcgcgaga ggccgccggc cgggggccgc acagcgggcg cagcgcctcg cgctcgtcgt
     1021 gcgagagcac ggtgatcgga tagaccggcg cgcgagttcc gcgatcgaag aacgggtgcg
     1081 gccaggtctg tccgcgcagc ttccagccga ggaccttgcg gatcgcgcgg gccaggagcc
     1141 ggacgttggg ccgggtggtg ccgggcggcg gggtgcccac ggcgaacttt ccgaggatgc
     1201 cgccgatcgg cgggccgaag tcgctgtcgg gggtgcggaa cgcctgggcg atcacgccga
     1261 cgaacgggat cccgggccct acggtgttgc ccagcggggt cttgcagcac atcgcgtacc
     1321 agcggtagag gcccttgggg gtcaggcgca gcccggcgat gcgatcggcg ccgcgcacga
     1381 agcggagcga cgccggcgcg acctggacga tgtcactgcc gccgtgcggg tcgagcaggt
     1441 cggcgcgccc gaggtggtgg gcataggcct ggcagtcgtc gcagtagcag acgacgcggt
     1501 tcacggtgtc gcgcgcggcg ttctcgaggc ggccctcgac ttcaccgcag cggcaatgca
     1561 gatcgacggt cggagtcatg gcagctcctg tgtcgtcgat ctcgggcggc cgagcaagcg
     1621 gcggcgccgg gcggcgtgcg ttcaggcgcc caccgcgatc cgctcggtct cgctgtcgga
     1681 gacgccggcg aggatgccct gcgcatactg ggcggtcgtg tatcccgatc cggcgatcga
     1741 ctcgtagcgc cacaggaagc cgccggtcag cgacgggaac tgcgtcctca gcgccgcgag
     1801 cgaggactgg atcgagcccg gcgtcgcgcc ctgggtgttg gcgtagccgg cgtacaggaa
     1861 cgcggccgga aagctgcgcg cccactgcgg gtacgacgca ccgccgtaga gctggaggtt
     1921 ccaccacgcg aagctgccgc ccgcgcgctg ggtctgctgc aggaccgtgc tccagaacgg
     1981 gatatcgtag tacggcgcgg cggtgacgag cttcccgagg ctgttcgccc agaccgtgag
     2041 ctggacgagc agggtggtga actggctcag gttctgctcg aggtcccagt ccagtccgtc
     2101 gatccggccc gccgccatga ccttggccat cgtcgcctgg aagctggcga ggttggcctg
     2161 gatggcggcg aagtcggagg cgaacgggcc gaaggtgacc agcaccctct gtaccttgcc
     2221 cgcctgcttg agcaggccgg gaaccgtctc gagcgcccac atcacggtgt cgagcggaga
     2281 gtcgttgtaa tagatgctgc cgttggagtg gacgtggaac tggccgagga tcacggtgga
     2341 gaacccggag tttccgatgt ccttggcagc ttccatcatc tgcttcatct gcgcgggcgt
     2401 gggattgggc gcggagaacg tgccctggca atagaggacg gcttcgggag tggacattgc
     2461 tggatcgctc ctgtcgttcg agtcagagct cggcggacgc ggcgtgcgtg cgcccgagtg
     2521 cgtgacaggc aatctaggca gcgcgggctg cccggaccat gactggccat tacggcagat
     2581 tacctgcgcc ggcggggtgc cgcgtcgatc tcccaagaca atcgagccgg acggcgccgg
     2641 cgagcccgtg gtatgcgacg ggcatgggcc acgagcgcgc caacatcgcc cgcatgcacg
     2701 gctacgtccc gggcaagcag ccggcgtcga gcgagacgat caagctcaac accaacgaga
     2761 acccgtaccc gccgtgcgac gcggtgatgc gcgagctggc ggcgatcccc gccgaggccc
     2821 tgcgccgcta tccgccgccg agcgccgagg gggttcgcga gcgcgccgcc gcgctgcacg
     2881 ggctcgccag ggaccaggtg gtcgcggtca acggcggcga cgagctgctg cgcctggcgg
     2941 tgaccacgtt cgtcgagccg ggcggcccga tcggcgtgat cgagcccagc tactcgctgt
     3001 acccggtgct cgccgagatc cacgccagcc cggtggtccg ggccgacgcc gagcccgact
     3061 ggtcgtggcc ggccgatctc gccgagcgga tgaaccgcgc cggggtccgg ctgctgctga
     3121 tcgtcaaccc gcacgcgccg tcgggccacc tcgcgccggc ggcggcggtc gccgcgctcg
     3181 cccgcgcgtt ccacggcgtg ctgctggtcg acgaggccta cgtcgacttc gtcgatccgg
     3241 agctcggcca cgacgtggtc gggctgatcg cgcagcacga caacctgctg gtgctgcgca
     3301 cgctgagcaa gggctacagc ctcgccgggc tgcggttcgg ctacgggctc ggcgcggcgc
     3361 cgctgatcgc gccgctgttg tggaagacgc gcgacagcta caacgtcgac gcgatcgcgc
     3421 agcggctggc ggcggcggcg ctcgccgcgc gcgaccaggc c
//
LOCUS       DASVOR010001353         3330 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_247886,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001353 DASVOR010000000
VERSION     DASVOR010001353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_247886"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /gene="dnaN"
                     /locus_tag="VF516_25805"
     CDS             <1..579
                     /gene="dnaN"
                     /locus_tag="VF516_25805"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEX8111182.1"
                     /translation="GHRLSKVERTIPNGPKLSAGVIIPKKGLLEIKKVLEQGPNAKLA
                     VKTPHVFLVQDDIAIAVKLIDAQFPPYEQVIPRDHKKIITVDRMRLIDALRRAQLMSS
                     ETRGVKVAATKEGVTITSDNPDLVEVREELEAEYDSDPIAIGFNPKYVVELLSQMTSD
                     QVTVALGGELDPGLIRPLTGDDYLGVVMPMRI"
     gene            598..1704
                     /locus_tag="VF516_25810"
     CDS             598..1704
                     /locus_tag="VF516_25810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEX8111183.1"
                     /translation="MVATGVRNLRPLQLEPRERFNVFSGDNGQGKTNLLEAIFVVAAL
                     RSFRTSKLSDLIAFGAERAQLGARVRKDDLVRVYEVEIAPGARKVALDGKPVRPLARY
                     FGAFNVVVFTPEDLALPRGSPGDRRRFLDRGVFNLRPDYLATAQDYERVLKTRNTVLR
                     QAGDGAIDRRRLEDLLAVYDQQLARLGAQVIAARQAFVGVVQDELAAAFTAITRTERR
                     ASVRYVIRAEADRGASNGAASEDAILAAIRAARVKDLATRATQVGPHRDDLAFELDGR
                     EAGAYASQGQLRAIMLAWKTAELAVLARAHGDSPILLLDDVSSELDPQRNEYLFDHLA
                     GLAGQCFITTTHPGHVLLRQHRADYCIAGGEITE"
     gene            2002..>3330
                     /locus_tag="VF516_25815"
     CDS             2002..>3330
                     /locus_tag="VF516_25815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111184.1"
                     /translation="MRKRPGMYIGDTDDGSGLHHLVHEVVDNSVDEHLAGHCSRIEVV
                     IHFDNSVTVEDNGRGIPVDIMPGEGRPAAEVVMTVLHAGGKFGGKGYKVSGGLHGVGV
                     SAVNALSDWLELEIRRGGKVYYQKYSRGVPLDGLRQTGTTQSGERHGTKTTFHPDPEI
                     FKNVLEFSFEQLSTKLRELAYLNSGLEIVIVDERTDKRQVFKFEGGIATYVADLNANK
                     TPVSDVIAFTGTVGGGNGELAECFVDIAMQWNDGYSEIVTCFTNTIKNRDGGTHLTGF
                     RQALTRTINNYANEYKLLKEAKGGLSGEDLREGLSAVVSVKVSDPKYSNQAKDKLVSS
                     EVATAVSTVVTEKLGQYLEQHPKEARNIINKAVVAARAREAARKAREMVQRKGALELT
                     SLPGKLADCQERDPEKSEIFIVEGESAGGSAKQGRSRQFQAILPLRGKILN"
BASE COUNT          578 a         1131 c         1147 g          474 t
ORIGIN      
        1 ggccaccggc tgagcaaggt cgagcgcacg atccccaacg ggcccaagct gtcggccggc
       61 gtgatcatcc ccaagaaggg cctgctcgag atcaagaagg tcctcgagca ggggccgaac
      121 gccaagctcg cggtcaagac gccgcacgtg ttcctggtcc aggacgacat cgcgatcgcg
      181 gtcaagctga tcgacgcgca gttcccgccc tacgagcagg tgatcccgcg cgaccacaag
      241 aagatcatca cggtcgaccg gatgcggctg atcgatgccc tgcgccgcgc ccagctgatg
      301 tcgtcggaga cccgcggggt caaggtcgcc gcgaccaagg agggcgtgac gatcaccagc
      361 gacaaccccg acctcgtcga ggtccgcgag gagctcgagg ccgagtacga ctcggacccg
      421 atcgcgatcg gattcaaccc caagtacgtg gtcgagctgc tgagccagat gaccagcgac
      481 caggtcaccg tggcgctcgg cggcgagctc gaccccgggc tgatccggcc gctgaccggc
      541 gatgactacc tcggcgtggt catgccgatg aggatctagc gtctgcagat ccttgcgctg
      601 gtcgccaccg gcgtgcgcaa cctgcggccg ctgcagctgg agcctcgcga gcgcttcaac
      661 gtgttctcgg gcgacaatgg ccagggcaag acgaacttgc tcgaggcgat cttcgtggtc
      721 gctgcgctgc gctcgttccg gacctcgaag ctctcggacc tgatcgcgtt cggcgccgag
      781 cgcgcccagc tcggggcgcg ggtgcgcaag gacgacctgg tccgggtcta cgaggtcgag
      841 atcgcgccgg gcgcgcgcaa ggtcgcgctc gatggcaagc cggtccggcc gctggcgcgc
      901 tacttcggcg cgttcaacgt cgtcgtgttc acgcccgagg acctggccct gccgcgcggc
      961 tcaccgggcg accgccggcg gttcctcgac cgcggggtat tcaacctgcg gcccgactac
     1021 ctcgccaccg cgcaggacta cgagcgcgtg ctcaagaccc gcaacaccgt gctgcgccag
     1081 gcgggcgacg gcgcgatcga ccgccggcgg ctcgaggacc tgctggcggt gtacgaccag
     1141 cagctcgccc ggctcggcgc ccaggtgatc gcggcccgcc aggcgttcgt cggcgtggtc
     1201 caggacgagc tcgcggcggc gtttacggcg atcacgcgca ccgagcgccg ggcgtcggtg
     1261 cgctacgtga tccgcgcaga agcggaccgc ggggcctcga acggcgctgc gagcgaggac
     1321 gcgatcctgg cggcgatccg cgcggcacgg gtcaaggacc tcgccacccg cgcgacccag
     1381 gtcggtccgc accgcgacga cctggcgttc gagctcgacg gccgcgaggc cggtgcgtac
     1441 gcctcgcagg gccagctgcg cgcgatcatg ctggcgtgga agacggccga gctggccgtg
     1501 cttgcacgcg cacacggcga ctcgccgatc ttgctgctcg acgacgtctc gagcgagctc
     1561 gacccgcagc gcaacgagta cctgttcgat cacctcgccg gactggcagg gcagtgcttc
     1621 atcacgacca cgcatccggg ccatgtgttg ctgcgccagc accgcgccga ctactgcatt
     1681 gccggcggcg agattaccga ataattacat gagtataggt gtgctgaaaa ggccgcgtgc
     1741 gccctttccg acgaggaatt gctgtggtac ctaacagcct ccgcgcgcgc aaggcctcgc
     1801 tagatggcag atcccactgt aggttcgcag acttctggtt ctagtgccgg cggttctggt
     1861 gccggctccc ggccgccgac gtccggcaca ggttctgggg gtgctgggga tgctgggccc
     1921 gcggatgcgc gtgacgacgg tgacgacggc gctgccggct acgacgccgg ctcgatcggc
     1981 gtcctcaagg gactggaggg tgtgcgcaag cgcccgggga tgtacatcgg cgacaccgac
     2041 gacggcagcg ggctgcacca cctcgtccac gaggtggtcg acaactcggt cgacgagcac
     2101 ctcgccgggc actgcagccg catcgaggtc gtgatccact tcgacaactc ggtgaccgtc
     2161 gaagacaacg gccgcggcat ccccgtcgac atcatgccgg gcgagggccg tccggccgcc
     2221 gaggtcgtga tgacggtgct gcacgccggc ggcaagttcg gcggcaaggg ctacaaggtc
     2281 tcgggcggtc tgcacggcgt cggcgtgtcg gcggtcaacg cgctcagcga ctggctcgag
     2341 ctcgagatcc gccgcggcgg caaggtctac taccagaagt actcgcgcgg cgtcccgctc
     2401 gacggcctcc ggcagaccgg gacgacgcag tcgggagagc gccacggcac caagaccacg
     2461 ttccaccccg atcccgagat cttcaagaac gtgctggagt tcagcttcga gcagctgtcg
     2521 accaagctgc gcgagctggc gtacctcaac agcggcctcg agatcgtcat cgtcgacgag
     2581 cggaccgaca agcgccaggt gttcaagttc gagggcggga tcgcgactta cgtcgccgac
     2641 ctcaacgcga acaagacgcc ggtgtccgat gtcatcgcgt tcaccggcac cgtcgggggc
     2701 ggcaacggcg agctcgccga gtgcttcgtc gacatcgcga tgcagtggaa cgacgggtac
     2761 tccgagatcg tcacctgctt caccaacacg atcaagaacc gcgacggcgg cacccacctc
     2821 acgggcttcc gccaggcgct gacccgcacg atcaacaact acgcgaacga gtacaagctg
     2881 ctcaaggagg ccaagggcgg gctgtccggc gaggacctcc gcgaggggct gtcggcggtg
     2941 gtctcggtca aggtcagcga tccgaagtac tcgaaccagg ccaaggacaa gctggtgtcg
     3001 tccgaggtcg cgaccgcggt gtcgacggtc gtcaccgaga agctcgggca gtatctggag
     3061 cagcatccca aggaagcccg caacatcatc aacaaggccg tggtcgcggc gcgcgcccgc
     3121 gaggcagcgc gcaaggcgcg cgagatggtg cagcgcaagg gcgcgctcga gctgacgtcg
     3181 ctacccggca agctcgccga ctgccaggag cgcgaccccg agaagagcga gatcttcatc
     3241 gtcgagggcg agagcgccgg cggctcggcc aagcagggcc gcagccggca gttccaggcg
     3301 atcctcccgc tgcgcggcaa gatcttgaac
//
LOCUS       DASVOR010001354         2267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_539962,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001354 DASVOR010000000
VERSION     DASVOR010001354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539962"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1467)
                     /locus_tag="VF516_25820"
     CDS             complement(<1..1467)
                     /locus_tag="VF516_25820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111185.1"
                     /translation="MDLLLAHGDDGSPILDHSTDPSTAAATANPAARRTERFLADITA
                     NADDLARQGWAVVAPADADGARLEALVRELIRVRAEDQGAEVLSIRVPPGMDSTAASA
                     WKASVYPTLYGEDETRRPRYLLILGDLDRVSLDTQHVLEQDGITGRLACADDDGYAAY
                     VHKLLAWQRAPSQQDRARALFYTVHDGSSATTAGYSKLIVPCHDRCMRAARDKPRTFP
                     ASAVESHGAESPDPDELLRLAAARHPSVLLSMSHGMGPPRRRRWSPAEARELQGAMSF
                     GAEGALEVRDLASAPFLPGGLWIYFACFGAGTPRTSAYHHWLQMLADQGMPELGPVNE
                     TLRGLDEAGGFVSGLGKAALANPNGPLAMLSHIDLAWSYSYEELRVGGEHDARRVSGS
                     NRSLNFFQVISKLVAGARVGAASLALKLRLGAVSAELNAHYDRCKRAGAVEGTSPDEL
                     LALGNLWMLHQDLLGFTLLGDPAVRLPLASPDEDEPGAA"
     gene            1535..>2267
                     /locus_tag="VF516_25825"
     CDS             1535..>2267
                     /locus_tag="VF516_25825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111186.1"
                     /translation="MKSERPSIELFLSRGDVGQALARGLNVRALAEAGEASTDVDDGV
                     GPKHYVNYGGDPGSLRDQGWGVIAPSGDVGDRLLALAKPLIDRRTADQDREVPVYRVP
                     PGMTEVQAAAWVDRKLVENKLDDAIPGYLLVLGRPDQVSFELQQVLATGFNVGRVGFD
                     ADAGYEAYIEKLLRSERAPARGPARAVYFTARDGTPATELGRRLLMQPCITDAEEQRG
                     GGRCAATEIAAIEGDDPARAADRLVE"
BASE COUNT          306 a          828 c          828 g          305 t
ORIGIN      
        1 cgccgcgccc ggctcgtcct cgtccgggct cgccagcggc agccggaccg cgggatcgcc
       61 gagcagcgtg aagccgagca ggtcctggtg cagcatccac aggttgccga gcgcgagcag
      121 ctcgtcgggg ctggtgccct cgaccgcgcc ggctcgcttg cagcgatcgt agtgcgcgtt
      181 gagctccgcg ctgaccgcgc cgagccggag cttgagcgcg agcgacgccg cgccgacccg
      241 cgcgcccgcg accagcttgc tgatcacctg gaagaagttg aggctgcggt tcgagccgct
      301 gacccgccgg gcgtcgtgct cgccgccgac ccgcagctcc tcgtagctgt agctccacgc
      361 caggtcgatg tggctcagca tcgcgagcgg gccgttgggg ttggcgagcg cggccttgcc
      421 cagccccgag acgaagccgc cggcctcgtc gagcccgcgc agcgtctcgt tgaccggccc
      481 gagctcgggc atcccctgat ccgcgagcat ctgcaaccag tgatggtacg cgctggtgcg
      541 cggcgtgcca gcgccgaagc acgcgaagta gatccacagc ccaccgggca ggaacggcgc
      601 cgacgccagg tcgcggacct cgagcgcgcc ctcggcgccg aagctcatcg cgccctgcag
      661 ctcgcgcgcc tcggccggcg accatcgccg gcgccgcggc ggccccatgc cgtggctcat
      721 cgacagcagc accgacgggt ggcgcgccgc cgccagccgg agcagctcgt cgggatccgg
      781 cgactcggcg ccgtgggact cgaccgcgct ggccgggaac gtcctcggct tgtcgcgcgc
      841 cgcgcgcatg cagcgatcgt ggcacggcac gatcagcttg ctgtacccgg cggtggtcgc
      901 gctcgatccg tcgtgcacgg tgtagaacag cgcccgcgcg cggtcctgct gcgacggcgc
      961 gcgctgccac gccagcagct tgtggacgta cgcggcgtag ccgtcgtcgt cggcgcacgc
     1021 cagccggccg gtgatgccgt cctgctccag cacgtgctgg gtgtcgagcg acacgcgatc
     1081 gagatcgccg aggatcagca ggtagcgcgg ccgccgcgtc tcgtcctcgc cgtacagcgt
     1141 cgggtacacc gacgccttcc acgcgctggc ggcggtggag tccatgcccg gcggcacccg
     1201 gatcgacagc acctcggcgc cctggtcctc ggcgcggaca cggatcagct cccgcaccag
     1261 cgcctcgagc cgcgcgccgt cggcgtcggc gggcgcgacc accgcccagc cctgacgcgc
     1321 gaggtcgtcc gcgttcgcgg tgatgtcggc gaggaaccgc tcggtgcgcc gcgccgccgg
     1381 gttcgccgtg gcggccgcgg tgctcggatc ggtcgagtga tcgagaattg gactcccgtc
     1441 gtccccgtgc gcgagcaaca gatccaaggg accctcccgc gatccgagta tgcatgttga
     1501 cggcgctggc ctcaatcgga gagcgtgact tccgatgaaa tccgagcggc cgtccatcga
     1561 gctgttcctg agccgcggcg atgtcggtca ggcgctggcc cgcgggctga atgtacgcgc
     1621 gctcgccgag gccggcgagg caagcaccga tgtggacgat ggcgtggggc ccaagcacta
     1681 cgtcaactac ggcggcgatc ccggctcgct gcgcgaccag ggctggggcg tgatcgcgcc
     1741 gagcggcgac gtcggcgatc ggctacttgc gctcgcgaag cccttgatcg atcgacgcac
     1801 cgcggaccag gaccgcgagg tgccggtgta ccgggtgccg ccgggcatga ccgaggtgca
     1861 ggccgccgcg tgggtggatc gcaagctggt cgagaacaag ctggacgatg cgatccccgg
     1921 ctacctgctg gtgctcggcc gaccggacca ggtctcgttc gagctccagc aggtgctcgc
     1981 gaccgggttc aacgtcggcc gcgtcgggtt cgacgccgac gccggctacg aggcgtacat
     2041 cgagaagctc ttgcgcagcg agcgcgcgcc tgcccgcggc ccggcccgcg ccgtgtactt
     2101 caccgcgcgc gacggcacgc cggccaccga gctcggccgc cggctgctga tgcagccgtg
     2161 catcaccgac gccgaggagc agcgcggcgg cgggcggtgc gccgccaccg agatcgcggc
     2221 gatcgagggc gacgatccgg cgcgcgccgc cgatcggctg gtcgagg
//
LOCUS       DASVOR010001355         2502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_14699,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001355 DASVOR010000000
VERSION     DASVOR010001355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14699"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="VF516_25830"
     CDS             <1..843
                     /locus_tag="VF516_25830"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX8111187.1"
                     /translation="LIQAANPINDESRSVADIQAAMLEKHLPMIHEAGKQGVQILCLQ
                     EIFNGPYFCPSQDRRWYDAAEPVPGPTLDKLAPLAKKYQMVMVVPLYEREQAGLYYNT
                     AAILDADGSYLGKYRKTHIPQVAGFWEKYFFRPGNLGYPVFKTRYATIGAYICYDRHF
                     PEGARALGLAGAEIVYNPSATVAGLSQYLWKLEQPAHAVANGYFVGALNRVGSEAPWN
                     IGKFYGNSYFCDPRGQIIACASEDKDELLVATLDLDLIEEVRRTWQFFRDRRPDAYGA
                     LVKD"
     gene            848..2260
                     /gene="hydA"
                     /locus_tag="VF516_25835"
     CDS             848..2260
                     /gene="hydA"
                     /locus_tag="VF516_25835"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HEX8111188.1"
                     /translation="MRTLIKDGTVVTASDSFVADVWIEGDKIVGLTHPSQRGAQTADQ
                     TIDAAGQYVIPGGIDAHTHMELPFGGTTASDDFDTGTVAAAHGGTTTIVDFAIQQKGG
                     SMRAGLDAWFAKAQGKAAIDYGFHMIMTEANPAALEDMAAMVREGVTSFKMFMAYPGV
                     FLVDDQQIFRAMLRAGELGALITMHAEIGLPIDVLVQRALAAGHTAPIYHALTRPEAA
                     EATGTERAIALAEMAGVPVYMVHLSAQRALERVMEARDRGLPAYAETCPQYLFCTEDD
                     LRGEPPGQGQGEWKGAGYVCTPPLRPRHHHDHLWRGLRNYDLQVVATDHCPFCMKDQK
                     ELGRGDFSKIPNGMPGVETRLYLLWEGVVAGKISLNRFVEITSTAPAKIFGLYGKKGC
                     LAIGSDADIVVWDPRREKVLGKDTLHMRIDYSPFEGRKVPASPSHVLSRGELIVQNDQ
                     WVGKQKQGRGQFLRRGTFAL"
     gene            complement(2276..>2502)
                     /locus_tag="VF516_25840"
     CDS             complement(2276..>2502)
                     /locus_tag="VF516_25840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111189.1"
                     /translation="AAGFPWHGPNLVAIGFQSDIPSMIESNFTAFPGLPGNLEVITET
                     GFALTTELAHLWRSSATLLWSPPIIVEIGV"
BASE COUNT          444 a          872 c          817 g          369 t
ORIGIN      
        1 ctgatccagg ccgccaatcc gatcaacgac gagtcgcgca gcgtcgccga catccaggcg
       61 gcgatgctcg agaagcatct gccgatgatc cacgaggccg gcaagcaggg tgtccagatc
      121 ctgtgtctac aggagatctt caatggcccg tacttctgtc cgagccagga ccgccggtgg
      181 tacgacgccg ccgagccggt gccggggccg acgctcgaca agctcgcccc gctcgcgaag
      241 aagtaccaga tggtgatggt cgtgccgctg tacgagcgcg agcaggccgg cctgtactac
      301 aacaccgcgg cgatcctcga cgccgacggc agctacctgg gcaagtatcg caagacccac
      361 atcccgcagg tcgccgggtt ctgggagaag tacttcttcc gcccgggcaa cctgggctat
      421 ccggtgttca agacccgcta cgcgacgatc ggcgcgtaca tctgctacga ccgtcacttc
      481 cccgagggcg cgcgggcgct cggcctggcc ggcgccgaga tcgtctacaa cccgtcggcc
      541 acggtcgccg ggctgtcgca gtacctgtgg aagctcgagc agcccgcgca cgcggtcgcc
      601 aacggttact tcgtcggcgc gctcaaccgg gtcggcagcg aggcgccgtg gaacatcggc
      661 aagttctacg gcaactcgta cttctgcgat ccgcgcggcc agatcatcgc gtgcgccagc
      721 gaggacaagg acgagctcct ggtcgcgacc ctcgatctcg acctgatcga agaggtccgc
      781 cgcacctggc agttcttccg cgatcgccgc cccgacgcct acggggccct ggtgaaggac
      841 tgacgccatg cgcacgctga tcaaggacgg caccgtcgtc accgcgtcgg acagcttcgt
      901 cgccgacgtc tggatcgagg gcgacaagat cgtcggtctc acccatccgt cgcagcgcgg
      961 cgcgcagacc gccgatcaga cgatcgacgc cgcgggccag tacgtcatcc ccggcgggat
     1021 cgacgcccac acccacatgg agctgccgtt cggtggcacc accgcgtcgg acgacttcga
     1081 caccggcacg gtcgcggccg cccacggcgg caccaccacg atcgtcgact tcgccatcca
     1141 gcagaagggc ggctcgatgc gcgccgggct cgacgcgtgg ttcgccaagg cacagggcaa
     1201 ggccgcgatc gactacggct tccacatgat catgaccgag gccaacccgg cggcgctcga
     1261 ggacatggcc gcgatggtcc gcgagggcgt gacgtcattc aagatgttca tggcctaccc
     1321 gggcgtgttc ctggtcgacg accagcagat cttccgcgcc atgctgcgcg ctggcgagct
     1381 cggcgccctg atcacgatgc acgccgagat cggcctgccg atcgacgtgc tggtccagcg
     1441 cgcgctcgcc gccggacaca ccgcgccgat ctaccacgcg ctgacccgcc ccgaggccgc
     1501 cgaggccacc ggcaccgagc gcgccatcgc gctggccgag atggccggtg tgccggtgta
     1561 catggtccac ctgtcggccc agcgcgccct ggagcgcgtg atggaagccc gcgatcgcgg
     1621 cctgccggcg tacgccgaga cctgtccgca gtacctgttc tgcaccgagg acgatctgcg
     1681 cggcgagccc ccggggcaag gacaaggcga gtggaagggg gcaggctacg tctgcacgcc
     1741 gccgctcagg ccgcgccacc accacgatca cctgtggcgc ggcctgcgca actacgacct
     1801 ccaggtcgtc gcgaccgacc actgcccgtt ctgcatgaag gaccagaagg agctcggccg
     1861 cggcgacttc tccaagatcc ccaacggcat gcccggcgtc gagacccgcc tctatctgct
     1921 gtgggaaggc gtggtcgcag gcaagatctc gctgaatcgc ttcgtcgaga tcacctcgac
     1981 cgcaccggcc aagatcttcg gcctgtacgg caagaagggc tgcctcgcga tcggcagtga
     2041 cgccgacatc gtggtctggg accccagacg cgagaaggtc ctcggcaagg acacgctcca
     2101 catgcggatc gactactcgc cgttcgaggg ccgcaaggtc ccggccagcc cgtcgcacgt
     2161 gctgtcgcgc ggcgagctga tcgtgcagaa cgaccagtgg gtcggcaagc agaagcaggg
     2221 ccgcggccag ttccttcgcc gcggcacgtt cgcgctgtag gggatccccg ctggctcaaa
     2281 cgccgatctc gacgatgatc ggggggctcc acagcagggt cgccgagctc ctccagaggt
     2341 gagcgagctc ggtcgtcagg gcgaaccctg tttccgtgat gacctccagg ttcccgggaa
     2401 gtccagggaa tgcagtgaag ttgctctcga tcatggaagg aatatcggac tggaaaccga
     2461 tagcgacgag attggggcca tgccagggaa agccagcagc gt
//
LOCUS       DASVOR010001356         2037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43390,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001356 DASVOR010000000
VERSION     DASVOR010001356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43390"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..1347
                     /locus_tag="VF516_25845"
     CDS             316..1347
                     /locus_tag="VF516_25845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111190.1"
                     /translation="MLRAPEPIHAVSGSPDHAFAAIGGSIVRVSSSGSADLAFDAGTP
                     IVALTAVPSGGVVFSTSLGTFFLGDNGEVARISKLCASAIEGHGDDLYFVFDAAGIVH
                     ASPASALATLPDIDENRVVAAAPAPAPFTPESPPRPPRPPPFVFPAGDFRDIRLTLFR
                     TSRGGYSESAVDSPSPSQSSSMSGSMSNGTGLRFERTASSGFPGLVWSLGLDFDLTTG
                     GVVKPHTTLYSMAAQLAFGYGVPLGHYVQVELVPYGEVVGDAIDHPFSSQVAWGSGVG
                     VGGRLQVLYTVDTGMQLGIAADYNFRWLSLAGSCDGCTTTGGFAVTSYNASVTTAQAS
                     IGLVVGKRY"
     gene            1667..>2037
                     /locus_tag="VF516_25850"
     CDS             1667..>2037
                     /locus_tag="VF516_25850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111191.1"
                     /translation="MTSSDGRDPHPAGPRSGEDPREAEERVGRSDQQPADRRKQREDG
                     EKRRAEGETQRADADPRAEGKAADAQHAPEQRGSERIEAPGAELAPAEHAQALDDHER
                     AMQRRDREDPDVLLDIPIVKV"
BASE COUNT          316 a          684 c          697 g          340 t
ORIGIN      
        1 ccttacctgg tcgtgcgcta tacgatcgtc ccgctcgcca ggctgccctc gggggccgat
       61 gcgaccccgt tccgcgtcgc cggcgccgat ccaatcgatg acctcgcgtg gaccacagag
      121 ggcgtcccgc tgctcattgt ccgcgcacag tttggaacat tgagcgagca tggctttgcc
      181 gggttcctgt cactgcccga gcccgggatg cgcgttgtgc cggcagcggt cgacaagctg
      241 tggctgttca cgccacgccc agcagcaggc acaggtcagc tgtacctgta caggcgtgac
      301 ggcggcctcg cactcgtgct gcgcgcgccc gagccgattc acgccgtgtc aggctcgccg
      361 gaccacgcat ttgccgcgat cggggggtcg atcgtgcgcg tgtcgtcgag tggttctgcc
      421 gacctcgcgt tcgacgctgg cacgccgatc gtcgcgctca ccgcggtccc atccgggggc
      481 gtcgtgttct cgaccagtct cggcacgttc tttctcggtg acaacggcga ggtggcgcgg
      541 atcagcaagc tctgcgcctc ggcgatcgag gggcacggcg acgatctgta cttcgtgttc
      601 gatgcggccg ggatcgtcca cgcgtcgccg gcttctgcgc tcgccacgct ccccgatatc
      661 gacgagaatc gcgtcgtcgc tgctgcgccg gctccggcgc cattcacacc cgagagcccg
      721 ccgcggccac cgcggccacc gccgttcgtg ttccccgccg gcgacttccg ggacatccgg
      781 ctcaccttgt tccgcacgtc gcgcggcggc tactcggagt cggccgtcga ctccccatcg
      841 ccatcgcagt ccagctcgat gtccggctcg atgagcaacg gaacaggcct ccgtttcgag
      901 cgcaccgcct cgagtggttt cccggggctc gtgtggtcgc tcgggctcga ctttgatttg
      961 acgacaggcg gtgtcgtcaa accgcacacg acactctaca gtatggccgc gcagctcgcg
     1021 ttcggctacg gcgttccgct cggtcactac gtccaggtcg agctggtgcc gtacggcgag
     1081 gtcgtgggtg atgcgatcga ccaccctttc tcgagccagg tcgcgtgggg cagtggggtc
     1141 ggtgtcggag ggcggctgca ggtgctatat accgtcgaca ccggcatgca gcttggcatt
     1201 gcggccgact acaactttcg gtggttgagc ctcgccggct cgtgtgacgg ctgcacgacg
     1261 actggcggct tcgctgtgac cagttacaac gcttcagtca cgacagcgca ggcaagcata
     1321 ggcctcgtgg tcggcaagcg gtattgagcg atgatgtccc gccttgttgt cttgctcgat
     1381 cacgcagacc acgccgtagg ccacgcatcg agaggacgcg cctgagcgct cgctgcgtca
     1441 ccggggcggt ggcgcatcga gcacgacccg tccgaggtcg aggaccttcc ccgattcgat
     1501 cgccaggccg gtcctggaga acggtcgcgc ggggggacga gccaccgaac agggactcgg
     1561 cgtacaccgt gtagctgccc ggggggcgat ttctcctcgg gatttcacgc gcggtcgcgc
     1621 gcgggcacat gcgatgcaac cacgttgctc atcgaacgag gtttcgatga ccagcagcga
     1681 tgggagagac ccgcatccag cgggacctcg ctccggcgag gatccgcgcg aggccgagga
     1741 gcgcgtgggg cggagcgacc agcagccggc cgaccgcagg aagcagcgag aagatggcga
     1801 gaagcggcga gccgagggtg aaacacagcg agccgacgcc gacccgcgcg ccgaggggaa
     1861 ggctgcggac gcgcagcatg cgcccgagca acggggcagc gagcgcatcg aggctcccgg
     1921 agccgaactg gcgccggcgg agcacgcaca ggcgctggat gaccacgagc gggcgatgca
     1981 gcgacgtgac cgggaagacc ccgatgtcct cctcgatatc ccgatcgtca aggtcga
//
LOCUS       DASVOR010001357         3077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_102314,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001357 DASVOR010000000
VERSION     DASVOR010001357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102314"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF516_25855"
     CDS             <1..265
                     /locus_tag="VF516_25855"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8111192.1"
                     /translation="ARLMALYAAWAIDTGAPDAADAASRARVHAGDVVARATVDAVQI
                     FGGYGFVNDYPVEKLMRDARAFEALHGDERLARVLAARAAQTG"
     gene            288..1508
                     /locus_tag="VF516_25860"
     CDS             288..1508
                     /locus_tag="VF516_25860"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8111193.1"
                     /translation="MDLFHDNPLLQGVRQMLGAFAKQMMRPIAIQHDREESMPWDLLK
                     AARSAGMSQTALLDGRKRLTGIDDDPDPNKPKSQARLGVAASEELGWGCAGLALALAG
                     SNLAAAPIARIGTQEQKDEFRKLLTGTDEHGHVKVAAMALSEPGTGSDISGLKTAARP
                     DGDHWIIRGSKQWITNGRSASAYVVWAQTEPAAGRAGVRGFLVARGTPGLVPGKKETK
                     LGIRASETAQVHFEDVRVHKDMMLGGAHSGDGPAGGLADTKKMLDSTRPAVGAQAVGI
                     ARAAYEFLLDRVRGGSLHGTPMASHQHIMFELADLHMEISASRLLVHRAAWMADHHLD
                     NAAEAPAAKAHAARTAQSVTHRAMVLLGEEALEPGHPVEKWYRDAKIYDIFEGTGQIQ
                     RRIIAKHLTGQNPT"
     gene            complement(1583..2662)
                     /locus_tag="VF516_25865"
     CDS             complement(1583..2662)
                     /locus_tag="VF516_25865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111194.1"
                     /translation="MSESKFFYAISTKGNVMNTLDVRLSLANLHCYDEADGAGSAEPY
                     LWTVFFKIDGDTTVVNSQLALQGTATVIGRPGNHHDLPNQNVNAGDNVPIPAVLGEFD
                     LRLKPIPLQSPVGSVTAVGGVVGIIAVLNEQDDTSDSAIAAGHNALDKAVRDSLNALI
                     PTLTFTHQEPTDAEIKAMTKKISDTVLAAIKNNVATWDFLKVAGDMDDPLGNAMFYFS
                     HSDLEARGISGIGFQQRFTNSDGDWELQGRATASPVGVTTGSLHIAISGIPNTGTIFP
                     VTVSGPGLSQSLNKDTTFTKLLPGTYTIRASNFTTGQPGKPGHQIHVAHPASQQVTVT
                     AGQVANPKVTYTSIVDDPIRNRRDR"
BASE COUNT          502 a         1047 c         1023 g          505 t
ORIGIN      
        1 cgcccggctc atggcgctgt acgccgcctg ggcgatcgac accggcgcgc ccgacgccgc
       61 cgacgccgcc agccgggcgc gcgtccacgc cggagacgtc gtcgcccgcg cgaccgtgga
      121 cgccgtgcag atcttcggcg gctacggctt tgtcaacgat tacccggtcg agaagctgat
      181 gcgcgacgcg cgcgccttcg aggcgctcca cggcgacgag cggctcgccc gcgtgctcgc
      241 ggccagggcc gcccagaccg gataggcggc cagcgtcagg agcccccatg gacctcttcc
      301 acgacaaccc cttgctccag ggcgtacgcc agatgctcgg cgcgttcgcc aagcagatga
      361 tgcgcccgat cgcgatccag cacgatcgag aagaatcgat gccgtgggat ctgctcaagg
      421 cagcccggag cgccggcatg agccagaccg ccctgctcga tggccgcaag cgcctgaccg
      481 gcatcgacga cgatcccgat ccaaacaagc cgaagagcca ggcgcggctc ggagtcgctg
      541 cgtccgagga gctcgggtgg ggctgcgccg gcctcgcgct cgcgctcgct ggctccaacc
      601 tcgcggccgc cccgatcgcg cggatcggga cccaggagca gaaggacgag ttccgcaagc
      661 tgctcacggg caccgacgag cacggccatg tcaaggtcgc cgcgatggcg ctctcggagc
      721 ccggcaccgg ctccgacatc tcgggcctca agaccgcggc ccgccccgac ggcgaccact
      781 ggatcatccg cggcagcaag caatggatca ccaacggccg gtcggcctcg gcctacgtcg
      841 tctgggcgca gaccgagccg gcggccggac gtgcgggcgt acgcggcttc ctcgtcgcgc
      901 gcggcacccc gggcctggtc cccggcaaga aggagaccaa gctcggcatc cgcgcctcgg
      961 agaccgcgca ggtccacttc gaggacgtcc gcgtccacaa ggacatgatg ctcggcggcg
     1021 cgcacagcgg cgatggaccg gccggcggcc tcgccgatac caagaagatg ctcgactcga
     1081 cgcgcccggc ggtaggcgcg caggccgtgg gcatcgcccg cgccgcctac gagttcctgc
     1141 tcgaccgcgt ccgcggcggc tcacttcacg gcaccccgat ggcgagccac cagcacatca
     1201 tgttcgagct cgccgacctg cacatggaga tctcggcgtc gcgcctcctg gtccaccgcg
     1261 ctgcatggat ggccgatcat catctcgaca atgccgccga ggcaccagcc gccaaggcgc
     1321 acgccgcccg caccgcgcag agcgtcaccc accgcgcgat ggtcctgctc ggcgaagagg
     1381 ccctcgagcc cggccatccc gtcgagaagt ggtaccgcga cgccaagatc tacgacatct
     1441 tcgagggcac cgggcagatc cagcgtcgca tcatcgccaa gcacctcacc ggccagaacc
     1501 cgacgtagcg cgatcttgtc ccctgctggc gaccgggtag ggccggtccg gccccggccg
     1561 cgctcgattc ccgagccgat ggtcatcgat ctcgacggtt ccgaattggg tcgtcgacga
     1621 tggacgtgta ggtaactttc gggttcgcga cctggccagc agtgaccgtc acctgctggg
     1681 atgcgggatg cgctacatga atctgatggc ctggtttccc gggctgtccc gtcgtgaagt
     1741 tgctcgcgcg aatcgtgtac gtgccgggaa gcagcttcgt gaatgtcgtg tccttgttga
     1801 gcgactggct caggcccggc ccggagacgg tcaccgggaa gatggtgccg gtgttcggta
     1861 tgccggagat cgcgatgtgc aagctgccgg tagtcacgcc caccggagat gccgtcgctc
     1921 ggccttggag ctcccagtca ccatcagagt ttgtgaaacg ctgctggaac ccgatcccgc
     1981 tgatgcctcg cgcttcgagg tcgctgtggg agaagtaaaa catcgcgttg ccgagggggt
     2041 cgtccatgtc cccggctacc ttcagaaagt cccaggtcgc gacattgttc ttgatggccg
     2101 ctagtaccgt atcgctgatc ttcttcgtca ttgccttgat ctcggcgtcg gtcggctctt
     2161 gatgcgtgaa ggtgagcgtc gggatgagcg cattcaggct atcgcgtacc gccttgtcca
     2221 gcgcgttgtg gccggcggcg attgccgagt cgctggtgtc gtcctgctcg ttgagcaccg
     2281 cgatgatgcc cacgactccg cccaccgccg tcacgctccc gacggggctc tgcaacggga
     2341 tgggtttcag ccggagatcg aactctccga ggaccgccgg gatcggcaca ttgtcgccag
     2401 cgttgacatt ctgattgggc aggtcgtgat gattgcccgg cctgccgatg accgtggcag
     2461 taccctggag ggcgagttgc gagttgacga cggtcgtatc gccgtcgatc ttgaagaata
     2521 cggtccagag gtagggctcg gcgctgcctg cgccgtctgc ttcatcatag caatgaagat
     2581 tggccaaact cagcctgaca tcgagtgtat tcatgacatt tcccttggtg ctgatcgcgt
     2641 aaaagaattt cgactcgctc acatgcaggt tctctgcatt cccgggcgag cagcacacgt
     2701 cgtgccagca gtggtcgcgc cgccgccgcg ggcggccacc agcggcagct agcgaggtcc
     2761 tcgactctcg ccttcaggcg agggtaggtt ttcgtcgtgg tgcggtggct ggtgcaccgg
     2821 cggtgtacca gcccaccgga tggtacacgg gcgatggctg gtacgcgggc ggtggctggt
     2881 agatatcgat ggcgatggtg cgccggagat cgtgaccgat ttgggcgaga tcgccgacgg
     2941 cggctacatc ccctggatca aggacctgcg atggcggcct acttgaccgg ccagcacacg
     3001 atgctggacg gcagcgatgc cccgcgccga ccagccacgc gcgcgcgcac cagccgcggg
     3061 cgaacgcgcc ggggccg
//
LOCUS       DASVOR010001358         1572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_102318,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001358 DASVOR010000000
VERSION     DASVOR010001358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102318"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VF516_25870"
     CDS             <1..838
                     /locus_tag="VF516_25870"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8111195.1"
                     /translation="VRSGMTPIEQQTILVTGATDGHGKGLAVELVRRGATVLVHGRDA
                     GRIERTLEELRGAAPGAKLRGYRADLASLAEVRALAAEVLAHEPRLDGLVNNAGIGTR
                     VPEGGRLTSRDGHELRFAVNYLATFLLTRLLLPRIRASAPARIVHVSSAGQQAIEFDD
                     VMLERGYSGVRAYCQSKLAQILFSNDLAEELAGTGVTSNALHPATYMPTKIVASPISA
                     LADGVQATLRLVADPSLDGVSGRYFDMTRERRADAQAYDAEARRSLRALSEQLTGLAQ
                     GT"
     gene            835..>1572
                     /locus_tag="VF516_25875"
     CDS             835..>1572
                     /locus_tag="VF516_25875"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX8111196.1"
                     /translation="MRGPPAAILAVLTACATSSVWTESDVVFTRYSPLSRNAEIARRA
                     LPPLTYQRFQQTLASQGLTLSEQAIDLAKERFELYVPSGPPPAQGYAVLVFVSPSRRA
                     AQPRFWRALLDRRGMIFVSAHSSGNDENILDRRVPLALLAYENVRARYPIDAERVYVG
                     GLSGGGRVAEIVALAYPDVFRGALLNAGADPIDGQEGMYKPPAELFRQFQHSRLVYAT
                     GERDEGVLAQDEVSRRSMHDACVFDVKI"
BASE COUNT          213 a          490 c          652 g          217 t
ORIGIN      
        1 ggtacgctcg ggcatgactc cgatcgagca gcagacgatc ctggtcaccg gcgcgaccga
       61 tggccacggc aaggggctgg ccgtcgagct cgtgcggcgc ggcgcgacgg tgctggtgca
      121 cggccgcgat gcggggcgga tcgagcgcac gctcgaggag ctgcgcgggg cggcgcccgg
      181 ggcgaagctg cggggatacc gggcggacct ggcgtctctg gcagaggtgc gggcgctggc
      241 ggccgaggtg ctggcgcacg agccgcggct cgacgggctg gtgaacaacg ccgggatcgg
      301 aacgcgggtg cccgagggtg gccggctgac cagccgcgat ggccacgagc tgcggttcgc
      361 ggtgaactac ctggcgacgt tcctgctgac gcggctgctg ctgccgcgga tccgcgcgtc
      421 ggcgcctgcg cggatcgtgc acgtgagctc ggcgggacag caggcgatcg agttcgacga
      481 cgtgatgctg gagcgtggct actccggggt gcgggcgtac tgccagagca agctggcgca
      541 gatcctgttc tcgaacgatc tggccgagga gctggcggga accggggtga ccagcaacgc
      601 gctgcacccg gcgacgtaca tgccgacgaa gatcgtggcg tcgccgatca gcgcgctggc
      661 ggacggggtg caggcgacgc tgcggctggt ggcggatccg tcgctcgacg gggtgagcgg
      721 gcggtacttc gacatgacgc gcgagcggcg ggccgacgcg caggcctacg acgcggaggc
      781 gcggcgctcg ctgcgcgcgc tgtcggagca gctgaccggg ctggcgcagg gcacatgagg
      841 gggccgccgg ccgcgatcct ggccgtgctg accgcgtgcg cgacgtcgtc ggtgtggacc
      901 gagagcgacg tggtgttcac gcggtactcg ccgctgtcgc gcaacgcgga gatcgcgcgg
      961 cgcgcgctgc cgccgctgac ctaccagcgg ttccagcaga cgctggcgtc acaggggctg
     1021 acgctgtcgg agcaggcgat cgacctggcg aaggagcggt tcgagctgta cgtgccttcg
     1081 gggccgccgc cggcgcaggg gtacgcggtg ctggtgttcg tgtcgccgtc gcggcgtgcg
     1141 gcgcaaccgc ggttctggcg ggcgctgctc gaccgccgcg gcatgatctt cgtcagcgcg
     1201 cacagctccg gcaacgacga gaacatcctc gaccgccggg tgccgctcgc gctgctggcg
     1261 tacgagaacg tgcgggcgcg gtatccgatc gacgccgagc gggtgtacgt cggcgggctg
     1321 tcgggaggag ggcgggtcgc ggagatcgtc gcgctggcgt acccggatgt gttccgcggc
     1381 gcgctgctca acgcgggggc ggatccgatc gacgggcagg aggggatgta caagccgccg
     1441 gccgagctgt tccggcagtt ccagcactcg cggctggtct acgcgaccgg cgagcgggac
     1501 gagggggtgc tggcgcagga cgaggtgagc cggcgctcga tgcacgacgc gtgcgtgttc
     1561 gacgtcaaga tc
//
LOCUS       DASVOR010001359         3527 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_72223,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001359 DASVOR010000000
VERSION     DASVOR010001359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72223"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VF516_25880"
     CDS             complement(<1..533)
                     /locus_tag="VF516_25880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8111197.1"
                     /translation="MSYETFETIQVAVDGALATVTVAREAALNALSSKVLSELTTACG
                     ELEVSTDVRVVILTGAGKAFIAGADIAEMRELTPRQAQAFAGMGGALAAAIEGSEKPW
                     IAAVNGFALGGGCELAMACDFVYASDQARFGQPEVKLGIIPGFGGTQRLARRVGIAKA
                     KELCMTGDMIDAAEALR"
     gene            complement(611..1486)
                     /locus_tag="VF516_25885"
     CDS             complement(611..1486)
                     /locus_tag="VF516_25885"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEX8111198.1"
                     /translation="MAAIQRIGVVGAGQMGQGIAQVAAQAGLDVLIVDAAPDFAQAGI
                     GKIKKQLDRLVEKARLDAAARDQVMARLRAGDALRDLLPCDVVIEAAPEREELKLQIF
                     KSLGDICKDEAILASNTSSISITKIAAASGRPDRVIGMHFMNPVPVMKLVEIVRGLPT
                     SQETFDTVVALAQRFGKTTIAARDIPGFIVNRMLIPLLNEACYGLYEGLGTAADIDTG
                     VRLGLNPPMGPLELSDLIGLDTCLAIAEVLHRELGDDKYRPCPLLRQYVAAGWLGKKV
                     GRGFYEYDSQGQKKA"
     gene            complement(1487..2662)
                     /locus_tag="VF516_25890"
     CDS             complement(1487..2662)
                     /locus_tag="VF516_25890"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8111199.1"
                     /translation="MRDVVIVGAARTAIGSFLGSLAQVPATKLGATAIKAAIERAGVA
                     PGDVGLVHMGMVLPAGVGQAPARQAALGAGLPQSVPCVTVNKVCGSGLEAVIGAARAI
                     AAGEVEIAVAGGMESMSNAPHVVRGLRGGVKMGALDTVDSMVHDGLWDPYSNQHMGNC
                     AELCAKDKGIPRSVQDEHAAESYRRAQDAIKQGHFKAEIVAVKVADKKGEVSVETDEE
                     PGRGNIEKLPGLRPSFQKDGTVTAGNASSLNDGAAAVVLMSADEAKRRGAKVLARLVA
                     SGYHAQAPEWFTTAPAPAIKNALARAKWDAKQVDLWEINEAFSVVSVANNRLLELDPA
                     KVNVWGGAVALGHPIGASGARILVTLLYAMAARGAKTGGASLCIGGGEGIALLVERP"
     gene            complement(2762..3157)
                     /locus_tag="VF516_25895"
     CDS             complement(2762..3157)
                     /locus_tag="VF516_25895"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HEX8111200.1"
                     /translation="MSVEKRVRAYLELATKDVEAAELLLAGANRYAAYHVQQSVEKVT
                     KALLLARGIEAGIEHRLEELCKRFADSDAWPERLRSFVGYSAYATAFRYPTPGGRIPA
                     DPPAEDLRRDMSALRALLAAVRVELLGTA"
     gene            complement(3147..3476)
                     /locus_tag="VF516_25900"
     CDS             complement(3147..3476)
                     /locus_tag="VF516_25900"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8111201.1"
                     /translation="MPLNESLDPPLERLVRHVVAVMKPLEVWLFGSRAENRARPTSDY
                     DLLVVMPDATPEPELDPVRAWRLGWEVQVTADIIPCTRSEFDEEKHEIDSLPRAAVLR
                     GRKLYER"
BASE COUNT          498 a         1333 c         1138 g          558 t
ORIGIN      
        1 atccgcagcg cctcggcggc gtcgatcatg tcgccggtca tgcacagctc cttggccttg
       61 gcgatgccga cccggcgggc gagccgctgg gtgccgccga accccgggat gatgccgagc
      121 ttgacctcgg gctggccgaa cctggcctgg tccgaggcgt agacgaagtc gcacgccatc
      181 gccagctcgc agccaccgcc gagcgcgaac ccgttgaccg ccgcgatcca cggcttctcc
      241 gagccctcga tcgccgcggc cagcgcgccg cccatcccgg cgaacgcctg ggcctggcgc
      301 ggcgtcagct cgcgcatctc ggcgatgtcg gcgccggcga tgaacgcctt gcccgcgccg
      361 gtcaagatca ccacgcgcac gtcggtcgac acctcgagct cgccgcacgc cgtggtcagc
      421 tcggagagca ccttcgacga cagcgcgttg agcgccgcct cgcgggcaac cgtgacggtc
      481 gccagcgcgc cgtcgactgc gacctggatg gtctcgaatg tctcgtagct catgggtgcc
      541 ttcactgttg ccgagcgcgc gccggtgtgg gaatggcgcc ggtcacggct cggtcatggt
      601 ttggccggac tcaggccttc ttctggccct gcgaatcgta ctcgtagaac ccccggccga
      661 ccttcttgcc gagccagccg gcggcgacgt actggcggag cagcgggcac ggccggtact
      721 tgtcgtcgcc gagctcgcgg tgcagcacct cggcgatcgc caggcaggtg tcgaggccga
      781 tcaggtcgga cagctcgagc ggccccatcg gggggttgag cccgagccgg acgccggtgt
      841 cgatgtccgc cgccgtgccc agcccctcgt acagcccgta acaggcctcg ttgagcagcg
      901 ggatcagcat ccggttgacg atgaaccccg ggatgtcgcg cgcggcgatc gtcgtcttgc
      961 cgaaccgctg cgccagcgcg accaccgtat cgaacgtctc ctggctggtc ggcaggccgc
     1021 gcacgatctc gaccagcttc atcaccggca ccgggttcat gaagtgcatg ccgatcaccc
     1081 ggtccggccg gcccgacgcc gcggcgatct tcgtgatcga gatcgacgac gtgttcgagg
     1141 ccaggatcgc ctcgtccttg cagatgtcgc ccaggctctt gaagatctgg agcttgagct
     1201 cctcgcgctc cggcgcggcc tcgatcacca cgtcgcacgg caacaggtcg cgcagcgcgt
     1261 cgccggcgcg cagccgggcc atcacctgat cgcgcgccgc cgcgtcgagc ctggccttct
     1321 cgaccagccg gtcgagctgc ttcttgatct tgccgatgcc ggcctgcgcg aagtccgggg
     1381 ccgcatcgac gatcagcacg tccagcccgg cctgcgccgc gacctgggcg atgccctgcc
     1441 ccatctgccc ggcgccgacc acgccgatcc gctggatcgc cgccatctac ggccgctcca
     1501 ccagcagcgc gatgccctcg ccgccgccga tgcacagcga cgccccgccg gtcttcgcgc
     1561 cgcgcgccgc catcgcgtac agcagcgtca ccaggatgcg cgcccccgac gcgccgatcg
     1621 ggtggccgag cgccaccgcg ccgccccaca cgttgacctt cgccggatcg agctcgagca
     1681 gccggttgtt cgccaccgac accaccgaga acgcttcatt gatctcccac aggtcgacct
     1741 gtttcgcgtc ccacttcgcg cgcgccagcg cgttcttgat cgccggcgcc ggcgcggtgg
     1801 tgaaccactc gggcgcctgc gcgtggtagc cgctcgcgac cagccgcgcc agcaccttgg
     1861 cgccgcgccg cttcgcctcg tcggcgctca tcagcaccac cgccgccgcc ccgtcgttga
     1921 gcgacgacgc gttgcccgcg gtcaccgtgc cgtccttctg gaacgacggc cgcagcccgg
     1981 gcagcttctc gatgttgccg cggccgggct cctcgtcggt ctcgacgctg acttccccct
     2041 tcttgtcggc gaccttgacc gccacgatct cggccttgaa gtgcccctgc ttgatcgcgt
     2101 cctgcgcgcg ccggtagctc tcggcggcgt gctcgtcctg cacgctgcgc gggatcccct
     2161 tgtccttggc gcacagctcg gcgcagttgc ccatgtgctg gttggagtac gggtcccaca
     2221 gcccgtcgtg caccatcgag tccaccgtgt cgagcgcgcc catcttgacg ccgccgcgca
     2281 gcccgcggac cacgtgcggc gcgttcgaca tcgactccat cccgccggcc accgcgatct
     2341 cgacctcgcc ggccgcgatc gcgcgcgccg cgccgatcac cgcctcgagc cccgagccgc
     2401 acaccttgtt cacggtcacg catggcacgc tctgcggcag ccccgcgccg agggcagcct
     2461 gccgggcggg cgcctggccc acgccggccg gcagcaccat gcccatgtgg accagcccga
     2521 cgtcgccggg agcgaccccg gcgcgctcga tggccgcctt gatcgcggtc gcgccgagct
     2581 tcgtggcagg gacctgtgcg agcgagccga gaaagcttcc gatggcggtg cgcgcggcac
     2641 cgacgatcac cacatcacgc atgaggtggg gataccccgc agctcccgaa cccgtcaagc
     2701 taccgcctgg acggcgcggg cgcacgttca gacgctggtc ggtccgacca gttacggtca
     2761 gttacgccgt gccgagcagc tcgacccgga ccgcggcgag cagcgcacgc agcgcggaca
     2821 tgtcacggcg aaggtcctcc gcgggcggat cggcggggat gcgaccgccc ggcgtcgggt
     2881 accggaacgc ggtggcgtac gcggagtacc cgacgaacga gcgcaatcgt tccggccacg
     2941 cgtcgctatc cgcgaagcgc ttgcacagct cctccaaccg atgttcgatc ccagcctcga
     3001 tacctcgcgc cagcagcaac gccttcgtga ctttctcgac ggattgctgc acgtggtacg
     3061 cggcgtagcg gtttgctcct gcgaggagga gctcggccgc ttcgacatcc ttggtcgcaa
     3121 gctcgaggta cgcccggacg cgcttctcaa cgctcataca gcttgcgccc gcgcaacacg
     3181 gccgctctcg gtaacgaatc gatctcgtgc ttctcctcgt cgaactcgct gcgggtgcac
     3241 gggatgatgt ccgcggttac ctgcacctcc cacccgagcc tccaggctcg caccggatcg
     3301 agctccggct cgggcgtggc atcgggcatc acgacgagga gatcatagtc gctcgtcggt
     3361 cgcgcccggt tctcggcgcg actaccaaac agccacacct ccagcggttt catgaccgcc
     3421 acgacgtggc gaaccagccg ctccaagggg gggtcgagcg attcgttcag tggcatgggt
     3481 ggagatgcga ccactccgtg gggcatatca ttctacgagg ataggtt
//
LOCUS       DASVOR010001360         3026 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_189583,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001360 DASVOR010000000
VERSION     DASVOR010001360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189583"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VF516_25905"
     CDS             complement(<1..777)
                     /locus_tag="VF516_25905"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8111202.1"
                     /translation="MTTSRHALGRALTGWPRGAALAALVLALGGWEPFRTADPDVEAG
                     NRAYADGRYDDAIAAYDRAARRGGIDADGLAFDRGTAEARKAEVVRDPAEKQRLVQRA
                     LDDLQQASHARDPRIRGAASYNRGNLLMGQDKLDDAIEAYKQALRDQPGLDDARLNLE
                     IALRRRQKQQQSQPQQGQGQGQGQGQGQGQGQGQGQGQGQGQQGQPGQGQGGANPPPG
                     GGSGQGSDEQGSGGQGAGSQSGGGQGANPSQSGGNGQGANP"
     gene            complement(774..1958)
                     /locus_tag="VF516_25910"
     CDS             complement(774..1958)
                     /locus_tag="VF516_25910"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8111203.1"
                     /translation="MTFATPWLVWVAAAVPVAVAVLQIYDRARRRALLGRLGEQPVVG
                     KVIASASPGRRIAKDALAGAAVTLIALAAARPQIAGTRQVELHGLDLVVAVDVSKSML
                     VDDVGPTRETTARHLESSRLARARELARAVIDELPGDRIAPVVFAAAAAHFPLTEDHV
                     VASNFIRDLGPTDLPPGSNLSEVFRVSRCLLRPDLYDDLGCARIGRRGHGGDPLHGES
                     LDPRPREQGRQPHEAIEQKVERGKAIVLFTDGGDSDPAALREVALARELGIAVLLVGI
                     GTPAGGVVYDIDELTGRRTAPKRLPDGSTVTSRRDDAGMTALAAAGGDAKRYFIAADH
                     GEVDPRPIVDALRAVNRGLATKQVKDLRDVYQPFLFTALMLLVIEATISTRRRRRYPE
                     AS"
     gene            complement(1955..>3026)
                     /locus_tag="VF516_25915"
     CDS             complement(1955..>3026)
                     /locus_tag="VF516_25915"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8111204.1"
                     /translation="RALVPWLLVAGLGAIAALGIARSFDAMRGAEAVVWRSPRAVILL
                     AAAGFVGVVAFHLHRRRAAAMAFSQTALVRRIGGLAWLSDLPGVLRVLAVAALAVALA
                     RPETYRTIKHEIDTIDIMIVVDMSKSMEETDLPRDRLDAAQRVIRRFLRRSTHDRIGL
                     VVFGQQAMLQCPLTLDTKLLEQIVADLAIGDVPELGTAIGDGLGLALSQLRDDRRDLA
                     PGEPPTKSKVVILLSDGDSNWVTRFEPDEAAQVARDMGVKVYTVLVGREESDLFGGTS
                     VNPATLRSIAALTGGEFFRATDYDSFDRGFQTVRSKLDTTKRVEIERVPDKQMFVPLA
                     LLALILLGLELVLSHTRLRRLP"
BASE COUNT          387 a         1144 c         1094 g          401 t
ORIGIN      
        1 cggattcgcg ccctgcccgt tgccgccgct ctgcgacgga ttggcaccct ggccaccgcc
       61 gctctgcgag cccgcgcctt gcccgccgga gccctgctcg tcggagccct gaccgctgcc
      121 gccgcccgga ggcgggttcg cgccgccctg gccctgcccc ggctggcctt gctgaccttg
      181 cccctgaccc tgaccctggc cttgcccctg accctggcct tgcccctgac cctgaccctg
      241 accctgcccc tgctgcggct gcgactgctg ctgcttctgt cgtcgccgca gcgcgatctc
      301 caggttgagc cgcgcgtcgt cgagcccggg ctggtcgcgc agcgcctgct tgtaggcctc
      361 gatcgcgtcg tcgagcttgt cctggcccat cagcaggttg ccgcggttgt agctcgcggc
      421 gccgcggatc cgcggatcgc gggcgtgcga cgcctgctgg agatcgtcga gcgcgcgctg
      481 gaccagccgc tgcttctccg ccggatcgcg gacgacctcg gccttcctgg cctcggcggt
      541 gccgcgatcg aacgcgagcc cgtcggcgtc gatcccgccg cgccgcgccg cgcggtcgta
      601 ggcggcgatc gcgtcgtcgt agcggccgtc ggcgtaggcg cgattgccgg cctcgacgtc
      661 gggatccgcg gtgcgaaacg gctcccagcc gccgagcgcg aggaccagcg cggcgagcgc
      721 ggcgccgcgc ggccagccgg tgagggcgcg cccgagggcg tgcctcgagg tcgtcacgat
      781 gcctccgggt agcgccggcg ccgccgcgtg ctgatcgtcg cctcgatcac cagcagcatc
      841 agcgccgtga acaggaacgg ctgatagacg tcgcgcaggt ccttgacctg cttggtcgcg
      901 agtccgcggt tgaccgcgcg cagcgcatcg acgatcggcc gcggatcgac ctcgccgtgg
      961 tccgccgcga tgaagtagcg cttggcgtcg ccgcccgccg ccgcgagcgc ggtcatcccg
     1021 gcgtcgtcgc gccgcgacgt caccgtcgag ccatcgggca accgcttcgg cgcggtccgc
     1081 cggccggtga gctcgtcgat gtcgtagacc acgccgcccg cgggcgtgcc gatcccgacc
     1141 agcagcaccg cgatgccgag ctcgcgggcg agcgcgacct cgcgaagcgc cgccggatcg
     1201 ctgtcgccgc cgtcggtgaa cagcacgatc gccttgccgc gctcgacctt ctgctcgatc
     1261 gcctcgtggg gctggcggcc ctgctcgcgc ggccggggat ccaggctctc gccgtgcagc
     1321 ggatcgccgc cgtggccgcg ccggccgatc cgcgcgcagc cgagatcgtc gtacaggtcc
     1381 ggccggagca ggcagcgcga cacccggaac acctcggaga ggttggagcc cggcggcagg
     1441 tcggtcgggc cgagatcgcg gatgaagttc gacgcgacca cgtggtcctc ggtcagcggg
     1501 aagtgggcgg ccgccgcggc gaacaccacc ggcgcgatcc ggtcgccggg cagctcgtcg
     1561 atcaccgcgc gcgccagctc gcgggcgcgg gccagccgcg agctctcgag gtgacgcgcc
     1621 gtggtctcac gggtcggacc gacgtcgtcg accagcatcg acttggacac gtcgaccgcg
     1681 acgaccagat cgaggccgtg gagctcgacc tggcgggttc cggcgatctg cgggcgcgcc
     1741 gcggccagcg cgatcagcgt caccgccgcg ccggccagcg cgtccttggc gatccgccgc
     1801 cccggcgacg cgctcgcgat caccttgccg accaccggct gctcgccgag ccggccgagc
     1861 agcgcccgcc gccgcgcgcg gtcgtagatc tggagcaccg ccaccgccac cggcacggcc
     1921 gcggcgaccc acacgagcca gggcgtcgcg aacgtcacgg cagcctccgc agccgggtgt
     1981 gggacagcac gagctcgagg ccgagcagga tcagcgcgag cagcgcgagc gggacgaaca
     2041 tctgcttgtc ggggacccgc tcgatctcga cccgcttggt ggtgtcgagc ttgctgcgca
     2101 cggtctggaa cccgcggtcg aacgagtcgt agtcggtcgc gcggaagaac tcgccgccgg
     2161 tcagcgccgc gatgctgcgc agggtcgccg ggttgaccga ggtgccgccg aacagatcgg
     2221 actcctcgcg gccgaccagc acggtgtaca ccttgacccc catgtcgcgg gcgacctggg
     2281 cggcctcgtc gggctcgaac cgggtgaccc agttcgagtc gccgtcggac aagaggatca
     2341 cgaccttgct cttggtcggc ggctcgcccg gcgcgaggtc gcggcggtcg tcgcgcagct
     2401 gcgacagcgc gagcccgagg ccgtcgccga tcgcggtgcc gagctcgggg acgtcaccga
     2461 tcgccaggtc ggcgacgatc tgctcgagca gcttggtgtc gagcgtcagc gggcactgca
     2521 gcatcgcctg ctggccgaac acgaccaggc cgatccggtc gtgggtcgag cggcgcagga
     2581 accgccggat cacgcgctgc gcggcgtcga gccggtcgcg cggcaggtcg gtctcctcca
     2641 tcgacttgga catgtcgacg acgatcatga tgtcgatcgt gtcgatctcg tgcttgatcg
     2701 tgcggtaggt ctcgggccgc gccagcgcga cggcgagcgc ggcgaccgcc agcacgcgca
     2761 gcacgcccgg cagatccgac agccaggcca gcccgccgat ccggcgcacc agggcggtct
     2821 gcgagaacgc catcgcggcg gcgcgccggc ggtggagatg gaacgcgacc acgccgacga
     2881 acccggccgc ggcgagcagg atcaccgcgc gcggcgaccg ccacacgacc gcctcggcgc
     2941 cgcgcatcgc gtcgaacgat cgcgcgatcc cgagcgccgc gatcgcgccc agcccggcga
     3001 ccagcagcca cggcaccagg gcgcgg
//
LOCUS       DASVOR010001361         3326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_481735,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001361 DASVOR010000000
VERSION     DASVOR010001361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3326
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481735"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1368
                     /locus_tag="VF516_25920"
     CDS             <1..1368
                     /locus_tag="VF516_25920"
                     /inference="COORDINATES: protein motif:HMM:NF018455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HEX8111205.1"
                     /translation="PGSGKTILGNQVCFHRAAAGDSTIYITLLAESHTRMIAHLRTME
                     FFRPELIASAIYYISAFKVLEESGLDGLLRSLQEAVRARKAAFVVLDGLVSAEEFAPT
                     ARAYKKFIHELQTICSMTGCTVLLLSSTERAAGFRPEHTMVDGLIELGDTVVGVQSLR
                     HVVVRKMRGTRQLRGRHAMEVSSAGIKVRPRIETLLHLPDEDDPAPRQMAQVDFGVIG
                     LDAMLRGGLPGGSNTIVLGPSGSGKTMLGLQFLSTGAREDQIGLYLGFYERPASLLAK
                     GARLGLGLDAARERGVLHLLWEPPIEGGLDAVVERVLRAVVDRRVTRLCFDGLHGFRH
                     HAEYPERTRAVFSAFAAELTRLGVTTLFTLETGDIVSAGIAIPIDGVSALADNIILLR
                     HVELRSQLYRLLSILKVRDRAYDGAIREFQITDRGVVVADTFDSAEQILTGTARTVAD
                     PESQR"
     gene            1383..1748
                     /locus_tag="VF516_25925"
     CDS             1383..1748
                     /locus_tag="VF516_25925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8111206.1"
                     /translation="MTQSVLIVDDEFGLADITADLLREVGYDVRLAINGKLGLDSLAE
                     HPAHLVLTDLMMPVMDGPELIRRMRADPRFAAIPVVLMTALPEAIPNGEGALHDAVLI
                     KPFSLAEVLAVVRRLLPPA"
     gene            1806..2255
                     /locus_tag="VF516_25930"
     CDS             1806..2255
                     /locus_tag="VF516_25930"
                     /inference="COORDINATES: protein motif:HMM:NF016152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEX8111207.1"
                     /translation="MAKPRSARPASPRRVTFAPAPYPRARVASQLAKVRALCRELPDV
                     TERPSHGSPTFFVGKRTFAYFLDNHHGDGRLALWCAAPDGAQAMLVDSDPEGYFLPPY
                     VAHRGWIGVRLDRDLPWNQVASVLETAHQMHYRPPRAAKPARRAARR"
     gene            complement(2274..2915)
                     /locus_tag="VF516_25935"
     CDS             complement(2274..2915)
                     /locus_tag="VF516_25935"
                     /inference="COORDINATES: protein motif:HMM:NF016212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEX8111208.1"
                     /translation="MAFVDQLEAFKSERLAPILGAGRTSLTADAPGDAAQLLQIALAN
                     EINVSELAAAWMPTTRELDVKLAFARQAGDEAGHFQLVADRLTALGFDVASFAPADNP
                     LFQYLRSLTTTVERIAAGLVTLESIAYGVNQNFMALCERRGDAETVRIYREYIQPDEQ
                     RHQQLGQQLIARYAVTPEHQQLAQATVARLLEIAGATRASAAARLGTACFPGC"
BASE COUNT          466 a         1195 c         1179 g          486 t
ORIGIN      
        1 cccggctccg gcaagaccat cctcggcaac caggtgtgct tccaccgcgc ggcggccggc
       61 gacagcacga tctacatcac gctgctcgcc gagtcgcaca cccggatgat cgcgcacctg
      121 cgcacgatgg agttcttccg gcccgagctg atcgcgtcgg cgatctacta catcagcgcg
      181 ttcaaggtcc tcgaggagtc cggtctcgac gggttgctca ggtcgctcca ggaggcggtc
      241 cgcgcgcgca aggccgcgtt cgtcgtgctc gacggcctgg tgtcggccga ggagttcgcg
      301 ccgaccgctc gcgcgtacaa gaagttcatc cacgagctcc agacgatctg cagcatgacc
      361 ggctgcaccg tgctgctcct gtcgtcgacc gagcgcgcgg cggggtttcg ccccgagcac
      421 accatggtcg acgggctgat cgagctcggc gacacggtgg tgggcgtcca gtcgctgcgc
      481 cacgtggtgg tgcgcaagat gcgcggcacc cgccagctgc gcggccgcca cgccatggag
      541 gtctcgtcgg ccgggatcaa ggtccggccg cggatcgaga ccctgctgca cctccccgac
      601 gaggacgatc cggcgccgcg ccagatggcg caggtcgact tcggcgtgat cgggctcgac
      661 gcgatgctgc gcggcgggct gcccggcggc tcgaacacga tcgtgctcgg cccgtcgggc
      721 agcggcaaga ccatgctcgg gctgcagttc ctgtcgaccg gggcgcgcga ggaccagatc
      781 gggctctacc tcgggttcta cgagcggccg gccagcctgc tcgccaaggg cgcccggctc
      841 ggcctcgggc tggacgcggc ccgcgagcgc ggcgtgctcc acctgctgtg ggagccgccg
      901 atcgagggtg ggctcgacgc ggtggtcgag cgcgtgctgc gcgcggtggt cgaccgccgc
      961 gtcacccggc tgtgcttcga cggcctgcac gggttccgcc accacgccga gtaccccgag
     1021 cggacccgcg cggtgttctc ggcgttcgcc gccgagctga cccggctcgg cgtcaccacg
     1081 ctgttcaccc tcgagaccgg cgacatcgtc agcgcgggca tcgcgatccc gatcgacggg
     1141 gtgtccgcgc tcgccgacaa catcatcctg ctccgccacg tcgagctgcg ctcgcagctc
     1201 tatcgcctgc tctcgatcct caaggtgcgc gatcgggcct acgacggcgc gatccgcgag
     1261 ttccagatca ccgatcgcgg cgtcgtggtc gccgacacct tcgacagcgc cgagcagatc
     1321 ctcaccggca ccgcccgcac cgtcgccgac cccgagagcc agcgctgagc cggacccagc
     1381 gcatgacgca atcggtcctc atcgtcgatg acgagttcgg gctcgccgac atcaccgcgg
     1441 atctgctccg cgaggtcggc tacgatgtcc ggctcgcgat caacggcaag ctcggcctcg
     1501 acagcctcgc agagcacccc gcgcacctgg tgctgaccga tctgatgatg ccggtgatgg
     1561 acgggccgga gctgatccgg cggatgcgcg ccgatccgcg gttcgccgcg atccccgtcg
     1621 tgctgatgac cgcgctgccc gaggcgatcc cgaacggcga gggcgcgctg cacgacgcgg
     1681 tgctgatcaa gccgttcagc ctcgccgagg tcctcgcggt cgtccggcgc ttgctgccgc
     1741 ccgcatgatc gccccgccgc ggtcggccga cctcccgacg cgccggcgtg ccacgatggc
     1801 ggcgcatggc caagcctcga tctgcccggc cggcgtcgcc tcgccgtgtc acgttcgcac
     1861 cggcgcccta tccgcgcgcg cgggtcgctt cccagctcgc caaggtccgc gcgctgtgcc
     1921 gcgagctgcc cgacgtcacc gagcggccga gccatggctc gccgacgttc ttcgtcggca
     1981 agcggacctt cgcgtacttc ctcgacaacc accatggcga cggccggctc gcgctgtggt
     2041 gcgccgcgcc cgacggcgcg caggcgatgc tggtcgacag cgatccggag ggctacttcc
     2101 tgccgcccta cgtcgctcac cggggctgga tcggcgtgcg cctcgaccgc gacctgccgt
     2161 ggaaccaggt cgccagcgtg ctcgagaccg cccaccagat gcactaccgg ccgccgcggg
     2221 cggccaagcc ggcgcgtcgc gcagccaggc gctgagaccc ggtccctcgg acctcagcag
     2281 cccgggaagc aggcggtgcc gagccgcgcg gcggccgagg cgcgggttgc cccggcgatc
     2341 tccagcaggc gggcgacggt ggcctgggcg agctgctggt gctcgggggt aaccgcgtac
     2401 ctggcgatca gctgctggcc gagctgctgg tggcgctgct cgtcgggctg gatgtactcg
     2461 cggtagatcc ggacggtctc ggcgtcgccg cggcgctcgc acagcgccat gaagttctgg
     2521 ttgacgccgt acgcgatcga ctccagcgtg accagccccg ccgcgatccg ctcgacggtc
     2581 gtggtcaggc tgcgcaggta ctggaacagc gggttgtcgg ccggcgcgaa gctcgcgacg
     2641 tcgaacccga gcgcggtcag ccggtcggcg accagctgga agtgcccggc ctcgtcgccg
     2701 gcctggcgcg cgaacgcgag cttgacgtcg agctcgcgcg tggtcggcat ccacgccgcg
     2761 gcgagctcgc tgacgttgat ctcgttggcg agcgcgatct gcagcagctg cgccgcgtcg
     2821 cccggcgcat cggcggtgag cgaggtgcgc cccgcgccga ggatcggtgc gagccgttcg
     2881 gacttgaagg cttccagctg gtcgacgaac gccatgccct tgcatagcac ccgcgcgcgg
     2941 tcgacggccg cggtgagcgc gtgaatacag gtggaagaat ggggctgcgc gaggcggccg
     3001 caagccggcc gcgggcaccg ggctgatcgg ccaggccaac gcggcgccgc aagcttgcag
     3061 tgccgcgtca caaggaaatc aaggagtacc tcgcccggag cctgcttcgg caggccgaga
     3121 tggcagattg atcggagcat cggagcacca ccatgatgat cacgggtcga ctctggaaag
     3181 ataggacggg gtggctcgcc gagtccgaga tcgcggacgt ctgcacgcag gggcgatcac
     3241 ggaaggacgc ggcgcggatg ctcgccgacg cgttcgagac gctgatcgat cggcccggtt
     3301 gcaaggtgac cgtcaccgat ggcggg
//
LOCUS       DASVOR010001362         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_364693,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001362 DASVOR010000000
VERSION     DASVOR010001362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364693"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /gene="rplB"
                     /locus_tag="VF516_25940"
     CDS             <1..411
                     /gene="rplB"
                     /locus_tag="VF516_25940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEX8111209.1"
                     /translation="TEIHAVELKIGRGAQIGRSAGTRVTLMAKEGDWATLRMPSGEMR
                     RVHIDCRATVGAIGNSEHGNIQWGKAGRMRWRGIRPHNRGVSMNPVDHPMGGGEGRSS
                     GGRHPCTPWGQKTKGLKTRHNKRTEQFIIRRRAK"
     gene            439..714
                     /gene="rpsS"
                     /locus_tag="VF516_25945"
     CDS             439..714
                     /gene="rpsS"
                     /locus_tag="VF516_25945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEX8111210.1"
                     /translation="MPRSIKKGAFVEHYLGKKMATAAKESNPARRVITTYSCRSTVIP
                     QMVGLTFAIHNGRKFIPVFITEQMVGHKLGEFSLTRTFHGHSGERKK"
     gene            717..1058
                     /gene="rplV"
                     /locus_tag="VF516_25950"
     CDS             717..1058
                     /gene="rplV"
                     /locus_tag="VF516_25950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEX8111211.1"
                     /translation="MQARALVRGISMSPRKMRVVANLVRNKKVEDAVGLLDLMPKRAA
                     GIIAKAIKSAAANAEERSGGDASIDQLRIHTIAVDGGTIAKRWMPRSMGRANRINHRT
                     SHLTVVVTDED"
     gene            1065..>1592
                     /gene="rpsC"
                     /locus_tag="VF516_25955"
     CDS             1065..>1592
                     /gene="rpsC"
                     /locus_tag="VF516_25955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEX8111212.1"
                     /translation="MGQKTHPTGFRLGIIKTWSSRWYEEKNYAKWLHEDLKLKDFIKK
                     KLNLAGVSYIEIERAANKAKINIHTARPGIVIGKRGAGVETLKKEVQALTENEVFLNI
                     IEVRKAETNAQLVAENIATQLERRIAFRRAMKKATQTAMKFGAKGIKVASSGRLGGAE
                     MCRREWYKEGRVPLHT"
BASE COUNT          329 a          549 c          516 g          198 t
ORIGIN      
        1 accgagatcc acgcggtcga gctcaagatc ggccgcggcg cgcagatcgg ccggtccgcc
       61 ggcacccggg tcacgctgat ggccaaggaa ggcgactggg cgaccctgcg catgccgtcg
      121 ggcgagatgc gccgggtcca catcgactgc cgggccacgg tcggcgcgat cggcaactcc
      181 gagcacggca acatccagtg gggcaaggcc ggccgcatgc ggtggcgcgg catccgcccg
      241 cacaaccgcg gcgtgtcgat gaacccggtc gaccacccga tgggcggcgg cgagggccgg
      301 tcgtcgggcg gtcgccaccc gtgcaccccg tggggccaga agaccaaggg cctcaagacc
      361 cgccacaaca agcggaccga gcagttcatc attcgccgcc gggcgaagta gcggaccagg
      421 acaccccagg acacaacgat gcctcgttca atcaagaagg gcgccttcgt cgagcactac
      481 ctcggcaaga agatggcgac ggccgccaag gagtccaatc cggcgcgccg cgtgatcacg
      541 acctactcgt gccgctcgac ggtgattccc cagatggtcg gcctcacgtt cgccatccac
      601 aacggtcgga agttcatccc ggtgttcatc accgagcaga tggtgggtca caagctcggt
      661 gagttctcgc tgacccggac attccacggc cacagcggcg agcggaagaa atagtcatgc
      721 aggcacgcgc actcgttcgc ggcatctcga tgtcgccccg caagatgcgg gtcgtcgcca
      781 acctcgtccg caacaagaag gtcgaggacg cggtcggcct gctcgacctc atgcccaagc
      841 gggccgccgg gatcatcgcc aaggcgatca agtcggccgc ggccaacgcc gaggagcgct
      901 cgggcggcga cgcgtcgatc gaccagctcc gcatccacac gatcgcggtc gacggcggca
      961 cgatcgccaa gcgctggatg ccgcgctcga tgggccgcgc caaccgcatc aaccaccgca
     1021 ccagccacct cacggtggtg gtcaccgacg aggactagcg ccacatgggc cagaagacac
     1081 atcccaccgg cttccgcctc ggcatcatca agacctggtc gtctcggtgg tacgaggaga
     1141 agaactacgc caagtggctg catgaagacc tgaagctcaa ggacttcatc aagaagaagc
     1201 tcaacctcgc cggcgtgagc tacatcgaga tcgagcgcgc cgcgaacaag gcgaagatca
     1261 acatccacac cgcgcgcccc ggcatcgtga tcggcaagcg cggcgcgggc gtcgagaccc
     1321 tcaagaagga agtccaggcg ctgaccgaga acgaggtgtt cctcaacatc atcgaggtcc
     1381 gcaaggccga gaccaacgcg cagctggtcg ccgagaacat cgcgacccag ctcgagcgcc
     1441 ggatcgcgtt tcgccgcgcg atgaagaagg cgacccagac cgcgatgaag ttcggcgcca
     1501 agggcatcaa ggtcgcgtcg agcggccggc tcggcggcgc cgagatgtgc cgccgcgagt
     1561 ggtacaagga gggccgcgtc ccgctccaca cc
//
LOCUS       DASVOR010001363         6331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43457,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001363 DASVOR010000000
VERSION     DASVOR010001363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6331
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43457"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VF516_25960"
     CDS             <1..298
                     /locus_tag="VF516_25960"
                     /inference="COORDINATES: protein motif:HMM:NF016955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HEX8111213.1"
                     /translation="KVAKCLLIAKVLVADGIMTDDERGFLDGVMAAEGLDADERRQVI
                     ELEGWDDAERLVRGLPIEQRRALVEQLVDAASADGRLSPHELATIRQLAADLGV"
     gene            complement(302..3520)
                     /locus_tag="VF516_25965"
     CDS             complement(302..3520)
                     /locus_tag="VF516_25965"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8111214.1"
                     /translation="MLAAPEGDRERAERALAAASSSHEHAVRFYEDDSRLTEAVTAFL
                     GDGLRAGDLVAAIATPAHREAFQRRLEAEGFDVEQLVTTGRLYFVDAHDALSKFMRGG
                     DPDPELFRTELGALIEQLSAQAKSRRLRAYGELVDVLWLSGQHSAALRLEELCNDLQD
                     RHSFHLLCAYSMAGFYKEPAEVHQVCASHTGVRATDPGDGGTSLTNELAQYASGLARE
                     IAHRSEVEIALRNALRELRHKEEQLRESEQQFRDFVENAAVGLHQIGPDGTILWANRA
                     ELELLGYTREEYIGRRISDIHADRGVINDILARLGRGEELHDYEARLRAKDGSIRHVL
                     ISSNIHTRDGRFINTRCFMRDITQRKQAEEALRAREAQLQTTIDALPLLVAYMNLDLR
                     YELVSAGYQRWFGIAKADILGKHLKEVIGPAAFEIASPNLRRAMTGEIVSYEAAMPYP
                     TGPRCIQATYIPQRDREGTVSGLVTVLIDVTERKNFERYRVAAAERAQRLLVITSALA
                     DAVTTTEVFAAVVDRVAAAVDASSAALWLLEDDGRTARLAHAIGYSQAILQAFAALPI
                     DMQPSLPHLDSLRNAEPIWISSPEALVRQYPHLASLIQPDRSSQLTCLPLVSDGRTLG
                     VLAVTIDQLREPAEEVHSFLTLVARYAGQAIERLRLHRDNVEARSHAEQLYRFAHSAA
                     LADNVEQVFECGLDAIAATLGTERAAVLMFDDAGVMRYRAWRGLSDHYRSVVEGRTPW
                     PRDATAPQPILVDDVEADTSLVHHLLPLFRSERVGALAFVPLITRGRLLGKLMIYYGE
                     RHRFSKTEIELAATIGSHLASLASRFAALAALEQTVRYSELFTGVLAHDLRNPLGAIT
                     TAAHLVLMRQEGEGDRSAKPLSRIVASGQRMERMIDQLLDFTRARVGGGIEILVRDAN
                     LADLCSQAIGEIELVYPDRSIQRSFVGDQGGTWDPDRLLQIISNLVANACQHGRPGSA
                     VTVRLDGRQPDAIVAEVHNLGTIPEALQPTLFDPFRGTRHRRDHSRGLGLGLFIVKEL
                     VRAHGGTVEVKSAEPEGTTFTIRMPRRAVAGPRAAVPLALSH"
     gene            4306..5328
                     /locus_tag="VF516_25970"
     CDS             4306..5328
                     /locus_tag="VF516_25970"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8111215.1"
                     /translation="MQLRRWGTATMYWLPRPPTRELTVGAAETCGLRLDDRSGRVSRL
                     HARLVRRQTRWLLRDEGSKNGVWIDGGRRGELMLTPGLEIRIGGVTLIAESPLLNALR
                     SFLARLLGWKNERTVMVDLALRTVRMAATRRTALMLCGDGDLVPIARAIHRHVRNSHR
                     PFIVCDPRRQAGKETVRSAENHAVGMEALAAAAGGSLCVRSRRLPPDFAQVVERLRDP
                     TSQVQLIVCAEAPKECERCRVTPLVIPPLADRQDEIDRIIHEYAHDAMVELATPRYVF
                     QAADHDWVREHAASSLPEIEKATLRLVALRASRNLSHAATQLGMAPVSLSRWIGRREM
                     PMEIVT"
     gene            5701..>6331
                     /locus_tag="VF516_25975"
     CDS             5701..>6331
                     /locus_tag="VF516_25975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8111216.1"
                     /translation="MSSRGLDPGKRTLTEQLPVQRSANGAGTTDRGAVQEAAAQGIAS
                     GGGPLPHVDRIQRLFGRHDIRGVEAHVGGPAAVASRAIGAEAYATGHHVAFASPPTLH
                     TAANEAAHVVQQRGGVRLEGGVGESGDAHEQHANAVADVVVQGNSAETLLDRYASSSG
                     AGSPAVQRKPDDAPSPQQPEGAHVDRLIQLLAAPAAATAGHDDAYTLLSS"
BASE COUNT          942 a         2191 c         2272 g          926 t
ORIGIN      
        1 gaaggtggcg aagtgtctgc tgatcgcgaa ggtgctcgtc gccgacggca tcatgaccga
       61 cgacgagcgt ggcttcctcg acggcgtgat ggccgccgag gggctcgacg ccgacgagcg
      121 ccgccaggtg atcgagctcg aaggctggga cgacgccgag cgcctggtcc gcgggctgcc
      181 gatcgagcag cgccgcgcgc tggtcgagca gctggtcgac gccgcgtcgg ccgacggccg
      241 gctgtcgccc cacgagctcg cgacgatccg ccagctggcg gccgacctcg gggtgtgacg
      301 ctcagtggct cagcgcgagc ggcactgctg cgcgcggtcc ggcgacggcc cggcgcggca
      361 tccggatcgt gaacgtggtg ccctccggct cggcggactt gacctcgacg gtgccgccgt
      421 gggcccggac cagctccttg acgatgaaca agccgagccc gaggccgcgc gagtgatcgc
      481 ggcgatggcg ggtgccgcgg aacggatcga acagcgtcgg ctgcagcgcc tccgggatcg
      541 tccccaggtt gtggacctcc gccacgatcg cgtcgggctg ccggccgtcg agccggaccg
      601 tcaccgcgct gccgggccgg ccgtgctggc aggcgttggc gaccaggttc gagatgatct
      661 gcagcaggcg atccggatcc caggttccgc cctgatcgcc gacgaagctg cgctggatgc
      721 tgcggtcggg gtagaccagc tcgatctcgc cgatcgcctg gctgcataga tcggcgaggt
      781 tggcgtcgcg gaccaggatc tcgatcccgc cgccgacccg cgcgcgcgtg aagtcgagca
      841 gctggtcgat catgcgctcc atgcgctggc cgctcgccac gatccggctc agcggcttcg
      901 cgctgcgatc gccttcgccc tcctggcgca tcagcacgag gtgggctgcg gtcgtgatcg
      961 cgccgagcgg gttgcgcagg tcgtgcgcca gcacgccggt gaacagctcg ctgtagcgca
     1021 cggtctgctc gagcgccgcc agcgcggcga accgcgatgc gagcgacgcc aggtggctcc
     1081 cgatcgtggc ggcgagctcg atctcggtct tcgagaaccg gtggcgctcg ccgtagtaga
     1141 tcatcagctt gccgagcagc cggccgcgcg tgatcagcgg gacgaacgcc agcgcgccga
     1201 cccgctcgct gcgaaacagc ggcagcaggt ggtggaccag cgacgtgtcg gcctcgacgt
     1261 cgtcgaccag gatcggctgc ggcgcggtcg cgtcgcgcgg ccacggggtc cggccctcga
     1321 ccacgctgcg gtagtgatcg gacaggccgc gccaggcccg gtaccgcatc accccggcgt
     1381 catcgaacat cagcaccgcg gcgcgctcgg tgccgagcgt ggccgcgatc gcgtcgaggc
     1441 cgcactcgaa cacctgctcc acgttgtcgg ccagcgccgc ggagtgcgcg aagcggtaca
     1501 gctgctcggc gtgcgagcgc gcctccacgt tgtcgcggtg caggcgcagc cgctcgatcg
     1561 cctggcccgc gtagcgcgcg accagcgtga ggaagctgtg gacctcctcg gcgggctcgc
     1621 gcagctggtc gatcgtcacc gcgaggacac cgagcgtgcg gccgtcggag accagcggca
     1681 agcaggtcag ctgggacgag cggtcgggct ggatcagcga cgcgaggtgg ggatactggc
     1741 gcaccagcgc ctcgggcgac gagatccaga tcggctcggc gttgcgcagc gaatcgaggt
     1801 ggggcagcga cggctgcatg tcgatcggca gcgccgcgaa cgcctggagg atcgcctgcg
     1861 agtagccgat cgcgtgcgcg agccgggcgg tgcggccgtc gtcctcgagc agccacagcg
     1921 cggcgctcga tgcatcgacc gccgcggcga cccggtcgac caccgccgcg aacacctcgg
     1981 tcgtggtcac cgcgtcggcg agcgcgctgg tgatcacgag caggcgctgg gcgcgctcgg
     2041 ccgcggccac gcggtagcgc tcgaagttct tgcgctcggt gacgtcgatc agcaccgtga
     2101 ccagccccga gaccgtgccc tcccgatcgc gctgcgggat gtacgtcgcc tggatgcagc
     2161 gcgggccggt cggatacggc atcgccgcct cgtagctcac gatctcgccg gtcatcgccc
     2221 ggcgcaggtt cggcgaggcg atctcgaacg cggccgggcc gatcacctcc ttgaggtgct
     2281 tgcccaggat atcggccttc gcgatcccga accagcgctg gtatccagcg ctcaccagct
     2341 cgtaccgcag atcgagattc atgtaggcga ccagcagcgg cagcgcgtcg atcgtggtct
     2401 ggagctgcgc ctcgcgggcg cgcagcgcct cctcggcctg cttgcgctgg gtgatgtcgc
     2461 gcatgaagca gcgcgtgttg atgaaccggc cgtcgcgggt gtggatgttg gagctgatca
     2521 gcacgtgccg gatcgagccg tccttggcgc gcagccgcgc ctcgtagtcg tgcagctcct
     2581 cgccgcggcc gagccgcgcc aggatgtcgt tgatcacgcc gcgatcggcg tggatgtcgc
     2641 tgatcctccg gccgatgtac tcctcgcggg tgtagccgag caactccagc tcggcgcggt
     2701 tggcccacag gatcgtcccg tcgggcccga tctggtgcaa tccgaccgcg gcattctcga
     2761 cgaagtcgcg gaactgctgc tcgctctcgc gcagctgctc ctccttgtgg cgcagctcgc
     2821 gcagcgcgtt gcgcagcgcg atctcgacct cactccggtg cgcgatctcg cgcgcgagac
     2881 cactcgcgta ctgcgccagc tcgttggtca ggctggtccc gccgtcgccg ggatcggtgg
     2941 cccgcacccc ggtgtggctg gcgcagacct ggtggacctc ggcgggctcc ttgtagaacc
     3001 ccgccatcga gtatgcgcac agcaggtgga agctgtgccg gtcctgcaga tcgttgcaca
     3061 gctcctcgag gcggagcgcg gcgctgtgct ggccggacag ccagagcacg tcgacgagct
     3121 cgccgtacgc ccgcagcctc cgcgacttgg cctgcgccga cagctgctcg atcagcgcgc
     3181 cgagctcggt gcgaaacagc tcggggtcgg gatcgccgcc gcgcatgaac ttcgagagcg
     3241 cgtcgtgcgc gtcgacgaag tacagcctgc cggtggtgac cagctgctcg acgtcgaagc
     3301 cctcggcctc gagccggcgc tggaacgcct cgcgatgcgc gggagtcgcg atcgccgcca
     3361 ccagatctcc cgcgcgcagt ccgtctccaa ggaacgccgt cactgcctcg gtgaggcggg
     3421 agtcatcctc gtagaaccgg actgcgtgct cgtgcgaaga cgacgctgcg gcgagtgcgc
     3481 gttcggcgcg ctcgcgatca ccttccggcg ctgcgagcaa gccgtctaca gtcgcagccg
     3541 ctggctgcgc tcggtggctg atgtcgacca tggtcccccg gatgagtcgc gatgaagcga
     3601 cccacgcgag tgttgcacgc cgcgcgcgcg tcgagaaccc tccgcaacag atagaagctt
     3661 tcggtgtgcg ccgatgacac aggcgcggct ttcttgccgc gcgttgccac ggtgttgcaa
     3721 cgcaacgttt gcgtgcgagc cgtcggcgag gcgcgctcga cggaggcatc cgacgacact
     3781 tcgtgcgccg agcacggctc gtgctcggcg ctggcggatc ctcgggaccg cgagctctcg
     3841 tgcttcgtcg gacagcgtgc gagatccccc tccgggcagc gaccaggtgc gcgggccgat
     3901 gaagcgttgc ccgatcgtgc gatgcgcgct ccggccgagc ggttcgacgc gaatcgtcgc
     3961 gctgttccac tggcaaagcg tcgttcgtgc gcgctggttc gcatgggaag atcgcgtaat
     4021 cgagattgcg aatatcgacg tcagcggaag catcgaccca tgttcctgga cgaagcatcc
     4081 atgccagcgt ggagcttcga gcgccgattc gatacaaagc tgtcccggaa cgctcgacgc
     4141 gccctaacgc tgcgtgagat gggtggattc cgttgaccag ggcgtgcgga aaccgctacg
     4201 gaactcctcg ctcccatcgc aggaccgacg gtacggatga cgaccttgac acaaaccttc
     4261 acgcactccg acgccgccgg tggctgggcc atcgatgatg aggtggtgca gctgcgcaga
     4321 tggggaacgg cgacgatgta ctggctgccc cggccaccga cccgcgagtt gaccgtgggt
     4381 gccgccgaaa cctgcgggct ccggctcgac gatcgctccg gccgcgtgtc gcgcctgcac
     4441 gcgcgcctgg tccggcgcca gacccggtgg ttgctccggg acgaaggcag caagaacggg
     4501 gtgtggatcg acggcgggcg gcgcggtgag ctcatgctca caccggggct ggagatccgg
     4561 atcggcggcg tcacgctgat cgccgagagc ccgctcctga acgcgctgcg cagctttctg
     4621 gcccgtctgc tgggctggaa gaacgagcgc accgtcatgg tcgacctggc gctgcgcacg
     4681 gtccggatgg ccgcgacgcg ccggaccgcc ctgatgctgt gcggtgacgg cgatctcgtg
     4741 ccgatcgcgc gggcgatcca ccgccatgtc cggaactccc atcgaccgtt catcgtctgc
     4801 gatccacgcc ggcaagccgg caaggagacc gtccggtccg ccgagaatca cgcggtcggc
     4861 atggaagccc tcgcggccgc tgcgggcggc tcgctctgcg tgcgcagccg gcggctgccg
     4921 ccggacttcg cccaggtggt cgagcggctg cgcgatccga cctcgcaggt ccagctcatc
     4981 gtctgcgccg aggcaccgaa ggagtgcgag cgctgccgcg tgacgccgct cgtgatcccg
     5041 ccgctcgccg atcggcagga cgagatcgac cggatcatcc acgagtacgc ccacgacgcg
     5101 atggtcgagc tcgcgacgcc gcggtacgtg ttccaggcgg ccgaccacga ctgggtgcgc
     5161 gagcacgccg ccagctcgct gcccgagatc gagaaggcga cgctgcggct ggtcgcgctt
     5221 cgcgcgtcgc gcaacctcag ccacgccgcg acgcagctcg ggatggcccc ggtctcgcta
     5281 tcgcgctgga tcggccgccg cgagatgccg atggagatcg tgacgtgagg ccgaagaccg
     5341 cctcggccgc cggccacggt tgacggtcga tgtccggcca gtgatgaacg ctgtccggac
     5401 atcggacagt aggagattga gccgctacgc gcacccacgc cccggcatgg gcgccctcgc
     5461 cacagccagc tgtgagtaac cgtgagaccg acgagcgcat ggcgcgccgc cggcaatact
     5521 cggagacgaa cggcggcgtt cacgatggtc acctccggcg aggatgagcc ggcgcaaggc
     5581 gctgccggtc gtcctttgag ctgggagcac ggcgatcgcg gcgacggttc cgaggaatcg
     5641 gcgatgggta gtggattcga tcaacgacaa ggcgcgggcg gacaacgacg tccgggcggc
     5701 gtgagctcgc gtggcctcga ccccggcaag cggacgctca ccgagcagct cccggtgcag
     5761 cgttctgcga acggtgccgg gacgaccgac cgcggtgcgg tccaggaagc ggccgcgcag
     5821 gggatcgcca gcggaggcgg cccactaccc cacgtggaca ggatccagcg cctgttcggc
     5881 cggcacgaca tccgcggcgt cgaggcgcac gtcggcggac ctgccgcggt cgcgagccgc
     5941 gcgatcggcg ccgaagccta cgcgaccggg caccacgtgg cgttcgcctc gcctccgacc
     6001 ctccacaccg cggcaaacga agccgcgcac gtcgtgcagc agcgcggcgg cgtgcggctc
     6061 gaggggggcg tcggcgagtc cggggacgcg cacgagcagc acgccaacgc agtggccgac
     6121 gtggtcgtgc agggcaacag cgcagagacg ttgctcgacc ggtacgcgag cagcagcggc
     6181 gccggatcgc ctgcggtgca gcgcaagcct gatgacgcac cgtcgccaca gcagccggag
     6241 ggcgcgcacg tcgatcgctt gatccagctg ctcgccgcgc cggcggccgc aaccgctggt
     6301 cacgacgacg catacacgct gctgtcgtca c
//
LOCUS       DASVOR010001364         3980 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_452219,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001364 DASVOR010000000
VERSION     DASVOR010001364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452219"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /gene="nrfD"
                     /locus_tag="VF516_25980"
     CDS             <1..1635
                     /gene="nrfD"
                     /locus_tag="VF516_25980"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEX8111217.1"
                     /translation="PRARPRPAVLPRPRLGWYVLVALGLCLLALGLGAYAIQLQRGDI
                     VTDMRDPGRGGAAWGLYISLYVYLVGISFAGITVASIARLFKLHVLEPVTRLAELLTI
                     ASLIAGAIAVLADLGRPDHGLVSLPDLARPSSPFFGTFTLVVSGYLFSSVVAFFVTGR
                     RDAARMVRDGPRALRWFYRLWASGYRGTPEEHARHGRTTFWLALTILPLLVVAHSTLG
                     FIFGIQAGRPGWYSALMAPSFIVLAGASGMGMLILTAVVLRRMLGLRIPDESIHWLGG
                     FLWVLCLVYLYFTIVEELTATYAGPALDRHVAHAIVTGRFSRSFAVAIGCLAVGFLIP
                     FLQYLRARPSVPGLAIAAGLVNAGALVKRLLIVVPSQTHGAYLALEEGTYSPSWNEWA
                     ILAGISSLVLLIILSFARIFPLVHGEEPALERDTPLPRDPVRKLICLAWAAGALALVA
                     VGLTDSFRLWSGSELDPRLPLAPVIFATGVMMLFATAIIYEVLPSRRRRVRSARIVDR
                     ARTHTAPPRTALAAALHATERRPIPSDLPSSPRSQP"
     gene            1632..2567
                     /locus_tag="VF516_25985"
     CDS             1632..2567
                     /locus_tag="VF516_25985"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX8111218.1"
                     /translation="MTEAAHDVPVPPIHKLGLSVVSLVVLGLVSGGVAAGALAAGLEP
                     PLVMDVAGGGLVLGIYLALAPRVASQWERAVVLRLGKFIGLRGPGLFWIIPLLDRVAV
                     WVDVRVRTTGFAAERTLTRDTVPVNVDAVLFWQVADAERAALGLENYEEAVAWAAQTA
                     LREIIGKTDLADMLAGRDVLDASMQKLIDERARAWGITVTSVEIRDVVIPQALEDAMS
                     RRAQAEREKQARVILGDAEAEIAHKFSAAAGIYREDAIALQLRSLNLLYEGMKERGTL
                     MIIPSTMADSMGAGSYAGLAALAAQQPAGQLAAKK"
     gene            complement(2582..2731)
                     /gene="yacG"
                     /locus_tag="VF516_25990"
     CDS             complement(2582..2731)
                     /gene="yacG"
                     /locus_tag="VF516_25990"
                     /inference="COORDINATES: protein motif:HMM:NF015821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEX8111219.1"
                     /translation="MSKDPARPSKLFPFCSERCHLVDLGRWLGEDYRIPGPPAGLVAP
                     ASDDD"
     gene            complement(2755..>3980)
                     /locus_tag="VF516_25995"
     CDS             complement(2755..>3980)
                     /locus_tag="VF516_25995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8111220.1"
                     /translation="GLGAIGSPDEARALAASVPVLDDADLARVCDLVRAAAAAVERVQ
                     PAVVAPTTFHHPFTEIVGESAAVERVQPAPVAPTTFHHPFTEIVGESAAVRDVVKLLG
                     KVVKSEATVLVHGESGTGKELIARAIHYHGPRAKKPFVVQNCSAFNDNLLESALFGHV
                     RGAFTGAVKDQPGLFQVADGGTFFLDEIGDMSAALQVKLLRVLQEGTFMPVGGTKPVK
                     VDVRIIAASHKDLAAMVAGKQFREDLFYRVNVLKITVPPLRDRVADIPRLAQFFVAKH
                     GRAAKGAEAAAPPRLSQGALARLVAYPWPGNIRELENEIERMLVLAGDAPELTAAMVS
                     SRVAGGAHSVARALRDVIATAEADAIVAALGRHSGDRDSAAQDLGISRSYFDARSAAL
                     GLGAAGTGTGSGASP"
BASE COUNT          481 a         1473 c         1412 g          614 t
ORIGIN      
        1 cctcgcgccc ggccgcggcc cgccgtgctg ccccggccgc ggctcggctg gtacgtgctg
       61 gtcgcgctcg gcctgtgcct gctcgcgctc ggcctgggcg cctacgcgat ccagctccag
      121 cgcggcgaca tcgtgaccga catgcgcgac ccgggccgcg gcggcgccgc gtgggggctc
      181 tacatctcgc tctacgtcta cctggtcggc atcagcttcg ccggcatcac cgttgcgagc
      241 atcgcgcggc tgttcaagct ccacgtcctg gagccggtca cccggctcgc cgagctgctc
      301 acgatcgcgt cgctgatcgc cggcgcgatc gccgtgctgg cggacctcgg ccgtcccgac
      361 catggcctgg tgtcgctgcc ggatctcgcg cgaccgtcgt cgccgttctt cggcacgttc
      421 acgctcgtgg tctccggcta cctgttctcc agcgtggtcg cgttcttcgt gaccggccgc
      481 cgcgacgcgg cccgcatggt ccgcgacggg ccccgtgcgc tgcgctggtt ctaccggctg
      541 tgggcgagcg gctaccgggg cacgcccgag gagcacgccc gccacggccg caccacgttc
      601 tggctcgcgc tcacgatcct gccgctgctc gtcgtcgcgc actcgaccct gggcttcatc
      661 ttcgggatcc aggccggccg ccccggctgg tacagcgcgc tgatggcgcc gtcgttcatc
      721 gtgctcgccg gcgcatcggg tatggggatg ttgatcctca ccgccgtggt gctgcgccgc
      781 atgctcggcc tgcgcatccc cgacgagtcg atccactggc tcggcggctt cctgtgggtc
      841 ctgtgcctgg tctacctcta cttcacgatc gtcgaggagc tgaccgcgac ctacgccggc
      901 ccggcgctcg atcgccacgt cgcccacgcg atcgtgaccg gccggttctc ccgatcgttc
      961 gccgtcgcga tcggctgcct ggccgtgggc ttcctgatcc cgttccttca gtacctccgc
     1021 gcgaggccat cggtccccgg tctcgcgatc gcggccggcc tggtcaacgc cggggcgctg
     1081 gtcaagcgcc tgctgatcgt ggtgccgtcg cagacccacg gcgcgtacct cgcgctggag
     1141 gagggcacgt actcgccgtc gtggaacgag tgggcgatcc tcgccgggat ctccagtctg
     1201 gtcctgctca tcatcctgtc gttcgcgcgg atcttcccgc tggtccacgg cgaggagccg
     1261 gcgctggagc gcgacacccc gctgccgcgt gacccggtcc gcaagctgat ctgcctggcc
     1321 tgggccgccg gcgcgctcgc gctggtggcc gtgggcctca ccgactcgtt ccggctatgg
     1381 agcggcagtg agctcgatcc gcgactgccg ctggcgccgg tgatcttcgc gaccggcgtg
     1441 atgatgctgt tcgcgaccgc gatcatctac gaggttctgc cgtcgcgccg ccggcgcgtg
     1501 cgctcggccc gcatcgtcga ccgggcacgc acgcacaccg ccccaccccg caccgccctc
     1561 gccgcggcgc tccacgccac cgagcgacgc ccgatcccat ccgatctgcc ttcatcaccc
     1621 aggagccagc catgaccgaa gccgcgcacg atgtccctgt tcccccgatc cacaagctcg
     1681 gcctgagcgt ggtctcgctc gtcgtgctcg gcctagtcag cggcggcgtc gccgccggcg
     1741 cgctggcggc cgggctggag ccgccgctgg tgatggacgt ggccggcggc ggtctggtgc
     1801 tcgggatcta cctggcgctg gcgccccggg tcgcgagcca gtgggagcgc gccgtggtgc
     1861 tccggctcgg caagttcatc ggcctgcgcg ggcccgggct gttctggatc atcccgctgc
     1921 tcgatcgcgt cgccgtgtgg gtcgacgttc gcgtccgcac caccgggttc gccgccgagc
     1981 gcacgctgac ccgcgacacc gtgccggtca acgtcgacgc ggtgctgttc tggcaggtcg
     2041 cagacgccga gcgcgccgcg ctcgggctgg agaactacga ggaggcggtc gcgtgggccg
     2101 cgcagaccgc gctgcgcgag atcatcggca agaccgatct cgccgacatg ctcgccgggc
     2161 gcgacgtgct cgacgcctcg atgcagaagc tgatcgacga gcgcgcgcgc gcctggggca
     2221 tcaccgtcac ctcggtcgag atccgggacg tcgtgatccc gcaggcgctg gaggacgcga
     2281 tgtcgcggcg cgcccaggcc gagcgcgaga agcaagcccg cgtgatcctc ggtgacgccg
     2341 aggccgagat cgcgcacaag ttctcggctg ccgcggggat ctaccgcgag gacgcgatcg
     2401 cgctgcagct gcgatcgctc aacctgctct acgagggcat gaaggagcgc ggcacgctga
     2461 tgatcatccc gagcacgatg gccgactcga tgggcgccgg cagctacgcc gggctcgcgg
     2521 cgctcgccgc gcagcagccg gcgggccagc tcgccgccaa gaagtagctc ggccctggtg
     2581 gtcagtcgtc gtcggaggcc ggcgccacca ggccggccgg cgggcccggg atccggtagt
     2641 cctcgccgag ccagcggccg agatcgacca ggtggcagcg ctcgctgcag aacgggaaca
     2701 gcttgctggg ccgggccgga tccttgctca ccggcctttt gcaggtcggg caggtcatgg
     2761 gctggctccc gagccggtgc cggtgccggc ggcgccgagc ccgagcgcgg cgctgcgcgc
     2821 gtcgaagtac gaccggctga tgccgaggtc ctgcgccgcc gagtcgcgat cgccgctgtg
     2881 ccggccgagc gccgcgacga tcgcgtcagc ctcggcggtg gcgatcacgt cgcgcagcgc
     2941 gcgcgccacg ctgtgggcgc caccggcgac ccgcgacgac accatcgcgg cggtgagctc
     3001 gggggcatcg ccggcgagca ccagcatgcg ctcgatctcg ttctcgagct cgcggatgtt
     3061 gcccggccag gggtatgcca ccagccgggc gagcgcgccc tgcgacagcc gcggcggcgc
     3121 ggcggcctcg gcgcccttgg cggcgcgccc gtgcttggcg acgaagaact gggcgagccg
     3181 cgggatgtcg gcgacccggt cgcgcagcgg cggcacggtg atcttgagga cgttgacccg
     3241 gtagaacagg tcctcgcgga actgcttgcc ggcgaccatc gccgcgaggt ccttgtgcga
     3301 cgccgcgatg atccggacgt cgaccttgac cggcttggtg ccgccgaccg gcatgaacgt
     3361 gccctcctgc agcacgcgca gcagcttgac ctgcagcgcg gcggacatgt cgccgatctc
     3421 gtcgaggaag aacgtgccgc cgtcggcgac ctggaacagc cccggctggt ccttgaccgc
     3481 gccggtgaac gcgccgcgga cgtggccgaa cagcgcgctc tcgagcaggt tgtcgttgaa
     3541 cgccgagcag ttctggacca cgaacggctt cttggcgcgc gggccgtggt agtggatcgc
     3601 gcgcgcgatc agctccttgc cggtgccgct ctcgccgtgg accagcacgg tcgcctcgct
     3661 cttgaccacc ttgccgagca gcttgacgac gtcgcgcacc gcggcggact cgccgacaat
     3721 ctcggtgaac ggatggtgga acgtggtcgg cgcgaccggc gcgggctgca cccgctcgac
     3781 cgccgcggac tcgccgacga tctcggtgaa cggatggtgg aacgtggtcg gcgcgacgac
     3841 cgcgggctgc acccgctcga ccgcggcggc cgccgcacgg acgagatcgc acacccgggc
     3901 gaggtcggcg tcgtcgagca ccggcacgct ggccgccagc gcgcgggcct cgtcgggcga
     3961 accgatcgcg ccgagcccct
//
LOCUS       DASVOR010001365         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_43464,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001365 DASVOR010000000
VERSION     DASVOR010001365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43464"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VF516_26000"
     CDS             complement(<1..1056)
                     /locus_tag="VF516_26000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111221.1"
                     /translation="MRVLLSRPRGLTAAIAVALAALSHRAAAQRADVEANAAVNIGYS
                     QETQTVFVPDPGSSRPSDTPSSTAQRMFMEIRPGILVQTGSPRLIWRAGYIFAGTLTI
                     VGDSLSAASNQATGSLTAEISKFSFLTLTAVLSQGGTAFQLTSRSPDSGNAELRAPGS
                     PDTVAASAAESMITELARQVTMQQTLVASTNAPQSDLSARNSAIAGSLAIDRTFDRDA
                     IGAELHGSVSWLFPLQQGMKEYKSYASSALVHWNHDFSLNWNGMVRAGLEQIFTDTGS
                     EPLAFLPTGSLAVRYTPQPDVGLGLDFTHGTATNLQVGSVSLTDRLGVHGAIGIDSRK
                     SRAVAFSASALHNEPLGS"
     gene            complement(1201..>1943)
                     /locus_tag="VF516_26005"
     CDS             complement(1201..>1943)
                     /locus_tag="VF516_26005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111222.1"
                     /translation="LLGVDQATVRAVITQWGPGRFDAEVFMDGKALGPDGKPHPTLIP
                     PAFGLGGVNLHTWTGWGGVSHWNAFVANLEMHGQGTFFDPRLDDAVRFPIAAANHFGH
                     VSTPPNQDRITPHLAALQLYQLALAPPRPPTGSFSATAATRGKALFNGKARCAGCHVP
                     PLYTEPGWNMHTAAEIGIDSFQSDRSPDQRFRTSPLRGLFTHLKGGLYHDGRYATLGD
                     VVAHYNAAFALGLTAAESSDLVEFLKSL"
BASE COUNT          279 a          677 c          714 g          273 t
ORIGIN      
        1 gctgccgagc ggctcgttgt gcagcgcgct ggccgagaac gccacggcgc gcgacttgcg
       61 cgagtcgatg ccgatcgcgc cgtgcacgcc gagccggtcg gtgagcgaca ccgagccgac
      121 ctggaggttg gtcgcggtgc catgcgtaaa gtcgaggccc aagccgacat cgggctgcgg
      181 cgtgtagcgc accgccagcg agccggtcgg caggaacgcc agcggctcgc tgccggtgtc
      241 ggtgaagatc tgctccagcc cggcgcgcac catgccgttc cagttcagcg agaagtcatg
      301 gttccagtgg acgagcgccg agctggcgta gctcttgtac tccttcatcc cctgctgcag
      361 cgggaacagc cacgacacgc tgccgtgcag ctcggcgccg atcgcgtcgc gatcgaaggt
      421 ccggtcgatg gccagcgagc cggcgatcgc cgagttgcgg gccgacaggt cgctctgcgg
      481 cgcgttcgtc gaggcgacca gcgtctgctg catcgtgacc tggcgggcga gctcggtgat
      541 catcgactcc gccgcgctgg cggcgaccgt gtcggggctg cccggcgcac gcagctccgc
      601 gttccccgag tccggcgacc ggctggtcag ctggaacgcg gtgccgccct gcgacagcac
      661 ggccgtcagc gtcaggaagc tgaacttcga gatctcggcg gtcagcgagc cggtcgcctg
      721 gttggacgcc gccgacagcg agtcgccgac gatggtcagc gtcccggcga agatgtagcc
      781 cgcgcgccag atcaaccgcg gcgagccggt ctggaccagg atcccgggcc ggatctccat
      841 gaacatccgc tgcgcggtcg acgacggcgt gtccgacggc cgcgacgagc ccgggtccgg
      901 gacgaacacg gtctgggtct cctggctgta gccgatgttg accgccgcgt tggcctcgac
      961 gtccgcgcgc tgggctgccg cgcgatgcga cagtgcagcg agcgcgaccg cgatcgccgc
     1021 ggtcaggccg cgcgggcgtg acaggaggac gcgcatctgg cgcggcacga tagcagatcg
     1081 caggcacgcg gtcgccgcag gccggcgacg gcggcgcacg ttgccagccg cgcgcatgcg
     1141 ctcaccgcgc tgcaccacgc accgcgctgc acccgccgcg ccgtgagtat cagctgacga
     1201 tcacaacgac ttcaggaact cgaccaggtc cgaggactcg gccgcggtga gcccgagcgc
     1261 gaacgcggcg ttgtagtgcg cgaccacgtc gccgagcgtc gcgtaccggc catcgtggta
     1321 caggccgccc ttgagatggg tgaacaggcc gcgcagcggg ctggtccgga acctctgatc
     1381 cggcgagcgg tcggactgga acgagtcgat gccgatctct gccgccgtgt gcatgttcca
     1441 gcccggctcg gtgtagagcg gcggcacgtg gcagccggcg caccgcgcct tgccgttgaa
     1501 cagcgccttg ccgcgcgtcg ccgccgtcgc gctgaagctg ccggtcggcg gcctgggcgg
     1561 cgcgagcgcg agctggtaga gctggagcgc ggcgaggtgc ggcgtgatcc ggtcctggtt
     1621 cggcggcgtc gagacgtgcc cgaagtggtt cgcggccgcg atcgggaacc gcaccgcgtc
     1681 gtcgagccgc gggtcgaaga acgtgccctg gccgtgcatc tccaggttgg cgacgaacgc
     1741 gttccagtgg ctgaccccgc cccagccggt ccaggtgtgc aggttgacgc cgccgagccc
     1801 gaacgccggc gggatcaagg tgggatgcgg cttgccgtcc ggcccgagcg ccttgccgtc
     1861 catgaacacc tcggcgtcga accggcccgg cccccactgc gtgatcaccg cgcgcaccgt
     1921 ggcctgatcg accccgagca ggc
//
LOCUS       DASVOR010001366         1693 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_189606,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001366 DASVOR010000000
VERSION     DASVOR010001366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189606"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /locus_tag="VF516_26010"
     CDS             <1..1651
                     /locus_tag="VF516_26010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8111223.1"
                     /translation="AAYAVVPAGTAVTLMMTTGGRATLPSPDGAPWAYVFWARDLEGV
                     GGWVPAAELDGEPGGDAALQIRGLPVEDSPESGPQGGLRASRPGEQPAGAGPRDATRG
                     GVNGPALPDQRGRERPLGIVAARGRDVVHSTDAIGIAVPDPYTIVFETRDPTPYFVAL
                     TAGPGLRATPIEAWSRWPRHWDRPGRIVTSGPFHLAAWHERDRVELVRSPSYWDRGAV
                     RLARFTSLSIDDQAAATHLYFTGGCDATAANVIPSSYLPAINGEQRGGRAYKDYDVSP
                     FLGVYFLWIQTEKLANRHLRRALSLAIDRTAIPGFTHGGEIPTAQLTPGAPIARLSGA
                     DLAACGVRRDAPGVALVMAPGELCYVPPPGLDHDLAAARREIALARAEGGVPDRPIEY
                     RYNAGSEVHKQIAEYLQAAWAQIGVPVVLIAQEWNSLLDATARGEFEIARFGNVGTVI
                     DTESEFLALLRCGSPFNRGRYCSPVFERRMSEAHGMVDRRARNAVLRQAEAVVLDDAP
                     LVPLYVLTQKHLIKPYVRDYAINLLDLPPLWRIWIDPGGAR"
BASE COUNT          213 a          583 c          669 g          228 t
ORIGIN      
        1 cgcggcgtac gccgtggtgc cggcggggac tgccgtgacg ctgatgatga ccaccggcgg
       61 ccgcgcgacg ctgccgtctc ccgacggggc gccctgggcc tacgtgttct gggcgcgcga
      121 tctcgagggc gtcggcggct gggtgccggc ggccgagctc gacggcgagc ccggcggcga
      181 cgccgcgctc cagatccgcg gcctgccggt cgaggactcg ccggagagtg gtccccaggg
      241 cggcttgcga gcgtctcggc caggagaaca gcctgcaggt gcagggccgc gcgacgcgac
      301 gcgcggaggg gtgaatgggc cggcgttgcc ggaccagagg ggacgggaac gtcccctggg
      361 gatcgtcgcc gcgcgggggc gcgacgtcgt gcactcgacg gacgcgatcg ggatcgccgt
      421 gccggatccg tacacgatcg tgttcgagac ccgcgatccg acgccgtact tcgtggcgct
      481 gaccgccgga ccggggctgc gcgcgacgcc gatcgaggcg tggtcgcgct ggccgcggca
      541 ctgggatcgg ccggggcgca tcgtgacctc ggggccgttt cacctggcgg cgtggcacga
      601 gcgcgaccgg gtcgagctgg tgcgctcgcc gagctactgg gatcgcggcg cggtccggct
      661 ggcgcggttc acgtcgctgt cgatcgacga tcaggcggcg gcgacccacc tgtacttcac
      721 cggcgggtgc gacgcgacgg cggcgaacgt gatcccgtcg agctacctgc cggcgatcaa
      781 cggcgagcag cgcggcgggc gggcctacaa ggactacgac gtctcgccgt tcctcggggt
      841 gtacttcctg tggatccaga ccgagaagct ggcgaaccga cacctgcggc gcgcgctgtc
      901 gctggcgatc gaccgcaccg ccatcccggg gttcacccac ggcggcgaga tcccgaccgc
      961 gcagctcacg ccgggcgcac cgatcgcgcg gctgtccggc gccgacctcg cggcgtgcgg
     1021 ggtccgccgc gacgcgccgg gggtggcgct ggtgatggcg ccgggcgagc tgtgctacgt
     1081 gccgccgccc gggctcgacc atgacctggc ggcggcgcgc cgcgagatcg cgctggcccg
     1141 cgccgagggc ggggtgccgg accgcccgat cgagtaccgc tacaacgccg gcagcgaggt
     1201 ccacaagcag atcgccgagt acctgcaggc ggcgtgggcg cagatcggcg tgccggtggt
     1261 gctgatcgcg caggagtgga actcgctgct cgacgcgacc gcgcgcggcg agttcgagat
     1321 cgcccggttc ggcaacgtcg gcacggtgat cgacaccgag agcgagttcc tggcgctgct
     1381 gcgctgcggc tcgccgttca accgcggccg gtactgctcg ccggtgttcg agcggcggat
     1441 gagcgaggcg cacggcatgg tcgaccgccg ggcgcgcaac gccgtgctgc gccaggcgga
     1501 ggcggtcgtg ctggacgacg cgccgctggt gccgctgtac gtgctgaccc agaagcacct
     1561 gatcaagccg tacgtccgcg attacgcgat caacctgctc gacctgccgc cgctgtggcg
     1621 gatctggatc gacccgggcg gggcacggtg atccggttcg tcgccgggcg gctggccgcc
     1681 agtgtcgcgg tcg
//
LOCUS       DASVOR010001367         3269 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_218682,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001367 DASVOR010000000
VERSION     DASVOR010001367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218682"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..832)
                     /locus_tag="VF516_26015"
     CDS             complement(182..832)
                     /locus_tag="VF516_26015"
                     /inference="COORDINATES: protein motif:HMM:NF023363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3455 domain-containing protein"
                     /protein_id="HEX8111224.1"
                     /translation="MLLVSPNIARSELLSRITSIIAGFAGTLTLASCATDGTSGPLSA
                     GSTEQGVSVGQCPANTPAALAPAADQDLAFVLNAQGVQRYQCVQSTTGFAWTFVAPDA
                     DLFSPHPHQSVVHHFAGPTWLYKDSSSVVGVKTAGVTVDPAAIPWLLLNVTSHGGAEG
                     RLSDVTSIQRLQTTGGNAPATGCDADHVGAESDVLYTARYFFYRTSNGAHHVRCGG"
     gene            1388..1669
                     /locus_tag="VF516_26020"
     CDS             1388..1669
                     /locus_tag="VF516_26020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111225.1"
                     /translation="MELARVGRMEHLAFASEATLEQLCATIEGALLLPEMQLDFENET
                     GPDAAGQGAARYVVIFKGNPVPRVVYAEYPGAIVTAIQATGVPYAVASE"
     gene            complement(1799..2446)
                     /locus_tag="VF516_26025"
     CDS             complement(1799..2446)
                     /locus_tag="VF516_26025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111226.1"
                     /translation="MPAPVAVLAGLDPQPERRQVGLERVHRVARVDRREVARRHHEII
                     GGRRTAVDALPVRRLPRREDLAERRMYRHAPGDIGLRAAGVVARGDHQAPQRLQRRIV
                     VAPAQPGDLAMPQPRDRLERVLHAALGRNARVADQLLHLARGEGRRRAPLGILVDLGR
                     QLAPVEHARVALDQLVNDRIAKHRVHDPVYAEDRARRHVASLTPTHVGRAHAARR"
     gene            complement(2676..2747)
                     /locus_tag="VF516_26030"
     tRNA            complement(2676..2747)
                     /locus_tag="VF516_26030"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2712..2714),aa:Glu,seq:ctc)
     gene            complement(2822..2895)
                     /locus_tag="VF516_26035"
     tRNA            complement(2822..2895)
                     /locus_tag="VF516_26035"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2861..2863),aa:Gln,seq:ctg)
     gene            complement(2949..>3269)
                     /locus_tag="VF516_26040"
     CDS             complement(2949..>3269)
                     /locus_tag="VF516_26040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111227.1"
                     /translation="VVAVLAAAGRARAEPPVRLPPGTHTDAQGQLVSGRGLRDTSDFL
                     AAELARRGIDVQQIGPYAVRGVELTRFLSQAPSTPWLAIHILRIAGKTLIFFVPRGKN
                     PPTP"
BASE COUNT          554 a         1031 c         1203 g          481 t
ORIGIN      
        1 tgccaggctg gaagccgccc gcgaggttcg gcgcctgcgc cgcgccgagc tccaccagga
       61 ggcggcatga cccttctggt tccctgcttc aggctctgta accttcaccc agtatcgtca
      121 cgttcacgcc gaaccagtac atgacgacct ttggggcaga tgcgagagca ccgcagcggt
      181 gctatccacc gcagcgcacg tggtgggcgc cgttgcttgt gcgatagaag aagtatcgtg
      241 cggtgtacag cacgtcagac tcggcgccga cgtggtcagc atcgcacccc gtcgcgggag
      301 cgttgccgcc cgtcgtctgg aggcgctgga tcgacgtgac gtcggatagc ctgccttcgg
      361 cgccgccgtg gctggtgacg ttcagcaaca gccatgggat cgcggccgga tcgaccgtga
      421 cacccgcggt cttcacgccg accacgctgc tcgagtcctt gtagagccag gtcgggcctg
      481 cgaagtggtg gacgaccgat tggtgcgggt ggggcgaaaa caggtcggca tcgggcgcga
      541 cgaacgtcca cgcgaagccg gtcgtcgatt gcacgcactg atagcgctgt acgccctgcg
      601 cgttcagcac gaacgcgagg tcctgatcgg cggccggggc gagcgccgct ggcgtgttcg
      661 ccgggcattg gcccaccgac acgccctgct cggtcgagcc tgcgctgagt ggaccgctcg
      721 tgccgtcggt ggcgcacgac gcgagggtga gggttccggc gaagccggca atgatcgaag
      781 tgattcgaga caagagttcg ctccttgcga tattcggtga caccaggagc acactttggg
      841 ccaacgatca gcgacgcatc ccggcccgtc ttgagatgtc ctcgcaacag gatcactgaa
      901 aagttgaatg tcgaccaagg gcggcagggt tcgcggaatt ccatctcctg cgtcgtagcg
      961 gagacggttg cacgactcgc cccgcgaagc gcacgacgct gtaccagcaa caagcaacgt
     1021 cgtgtaccag gtacaggcgc ttcaggtgca ccggtacgtt tggccgatcg cggccctccg
     1081 caaagtcggc cgaggtcgcg gacgctgcca cggccgcgat cggcgagtcg ctccaccgag
     1141 gtcgccgccg aagtccagtt cggcatcgcg ggtcgccggg cgatggcgca tcgcgggagc
     1201 ggcgaggtcg gcggccgcgc gagaccggga gccaggggcg cgaggtggat tcggaacggg
     1261 tgcgagaccg ggagccaccg tggccacgag actgggagcc aggcgcgcat gaggccggga
     1321 gccgggcctc cggacgaacc cgcagcggag cggtccccgg acacgaccat gcgcgtatag
     1381 tcttggtgtg gagctggcgc gtgtcggccg catggagcac ctggcgttcg ccagcgaggc
     1441 gacgctcgag cagctgtgcg caaccattga gggcgcgctg ctgctgcccg agatgcagct
     1501 cgacttcgag aatgagaccg ggccggacgc tgcaggacag ggcgctgcca ggtacgtcgt
     1561 gatcttcaag ggcaatcccg tcccgcgagt cgtctacgcc gagtatcccg gtgcgatcgt
     1621 cacggcgatc caggcgaccg gggttccgta cgcggtcgcg tccgagtgac cggcgcgagc
     1681 gcacgcgcgg cgagctgcag cgggtcggcg gcgatcgcgg tggtggcgac gacctcgacc
     1741 ccgagctcgg tggcatgacc tcggcagcta gagccacagg aagcgcggat cgtaaaggtc
     1801 accgccgggc tgcgtgcgcg cgcccaacat gcgtgggcgt caggctggcg acatggcgcc
     1861 gcgcacggtc ctccgcgtat acgggatcgt gcacacgatg tttcgcgatg cggtcgttga
     1921 cgagctgatc gagagcaacc cgtgcatgct ccaccggggc gagctgccgg ccaaggtcga
     1981 caaggatccc gagtggcgca cgtcggcgac cttcaccacg cgcgaggtgg agcagctgat
     2041 cagcgacgcg cgcattccgc ccgagcgccg cgtgcagtac gcgctcaagg cgatcgcggg
     2101 gctgcggcat ggcgaggtcg ccgggctgcg ctggcgccac tacgatccga cgctggagcc
     2161 gctggggcgc ctggtgatcg ccacgagcta cgacaccggc cgcacgaaga ccgatgtcac
     2221 ccggcgcgtg ccggtacatc cgacgctcgg caagatcctc gcggcgtgga agccgtcgca
     2281 ctgggagcgc atctacggcc gtgcgccgac cgccgatgat ctcgtggtgc cgacgcgcaa
     2341 cctcacgacg atcgacgcga gcgacgcggt gcacgcgttc aaggccgacc tggcggcgct
     2401 cgggctgcgg atcgaggccg gcgagcaccg cgaccggggc gggcatgacc tgcgctcgtg
     2461 gttcatcacc acgtgccagg agcacggcgc acaccgggat ctcctgcggg tggtcacaca
     2521 cacctcgaag ggcgacgtgg tgagcggcta cacgcgcgcg cagtggccgg cgctctgcgc
     2581 cgaggtcgcc aagctgcggg tgtcgaccct cgacggagag atcctgccgc ttgctacagc
     2641 cgaggtaaag gccgcgaacc gctggagaaa agtagtgacc ccaatgggac ttgaacccat
     2701 gttttcggcg tgagaggccg acgtcctaac cgctagacta tggggccgag gaagggagac
     2761 gtggttctac ccgcggcggg cgaggcggtc aagcacccgc gggcattccg tgggtgcagg
     2821 ctggttcggg gacatggatt cgaaccatga tagtcagatc cagagtctga cgtcctacca
     2881 ttagacgatc cccgagtaga ccgagagaaa tcggtgggcc ctttagctaa ccggagcacg
     2941 gcgattcgtc aaggtgtggg cgggttcttg ccacggggga cgaagaagat caaggtcttg
     3001 ccggcgatcc ggaggatgtg gatggcgagc cagggcgtcg agggggcctg gctgaggaac
     3061 cgggtgagct cgacgccgcg gacggcgtag gggccgatct gctggacgtc gatgccgcgg
     3121 cgagcgagct cggcggcgag gaagtcactg gtgtcgcgca ggccgcggcc ggagacgagc
     3181 tggccctggg cgtcggtgtg ggtgccgggc gggaggcgca ccggcggctc ggcgcgcgca
     3241 cggccggcgg cggcgagcac cgcgacgac
//
LOCUS       DASVOR010001368         1838 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_452238,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001368 DASVOR010000000
VERSION     DASVOR010001368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452238"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="VF516_26045"
     CDS             complement(<1..299)
                     /locus_tag="VF516_26045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111228.1"
                     /translation="MGSPFAPVVLPIAALIAALVRWQLQGSHNLYTAVSKRFYVPDPD
                     LGWQVSPKHPLWIGLELCAVIAAIAAALVVAGWLLRRREARLGHRTAALRGATWLV"
     gene            complement(474..1325)
                     /gene="aguB"
                     /locus_tag="VF516_26050"
     CDS             complement(474..1325)
                     /gene="aguB"
                     /locus_tag="VF516_26050"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR03381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEX8111229.1"
                     /translation="MEVTVAALQTALTDDVAGNVARVTALCREAAGRGAQIVLPSELF
                     EGHYFCRDQREADFSRARPAEGHPTLRHFQQLAAELGVVIPVSFFEQAGPEYYNSIAV
                     LDADGRSLGVYRKSHIPDGPGYQEKFFFKPGNTGFRAFATRFGTIGVAICWDQWFPEA
                     ARAMTLAGADVLFYPTAIGSEPEEPELDTRDAWQRVMIGHAVANAVGVVAANRIGTEG
                     DGAGAITFYGSSFVADARGDKLAELGRSDAGIALARLDLTQIRRLRTSMGFFRDRRPG
                     LYRVLTE"
     gene            complement(1363..>1838)
                     /locus_tag="VF516_26055"
     CDS             complement(1363..>1838)
                     /locus_tag="VF516_26055"
                     /inference="COORDINATES: protein motif:HMM:NF016274.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEX8111230.1"
                     /translation="ALAVDKVVWLDRGLANDHTDGHIDTLARFVAPGVVACMEPAAGD
                     PNRDALAGILADLGAARTAGGDRLEVVTVPSPGAVEDAAGNLMPASYMNFYIANTVVV
                     VPTYGVRADHAAVKAIEAMFPTRRVIGVPGKPVVVGGGAFHCSTQQQPRVPPAIG"
BASE COUNT          283 a          634 c          673 g          248 t
ORIGIN      
        1 accagccacg tcgcgccgcg cagcgcagcg gtgcgatggc cgagccgggc ctcgcgccgc
       61 cggaggagcc agcccgcgac cacgagcgcc gccgcgatcg cggcgatcac cgcgcacagc
      121 tcgaggccga tccacagcgg atgcttcggg ctgacctgcc agccgagatc cggatccgga
      181 acatagaagc gctttgaaac cgccgtgtag aggttgtgcg atccctggag ctgccagcgc
      241 acgagcgccg cgatcagtgc agcgatcggc aacaccaccg gcgcgaacgg cgaacccacg
      301 agccttgggt aacctgttct tcgtcgcgat gcactctggc ggcgcgaaac attgtgttgc
      361 gtccgtcgga gatcacagcg tcgtgaggtt caggccggaa tcgccagagg accctgccgg
      421 agcgatgaat atcgaactgc gacgcagcgt cgcatggggt cggccacccg gtgctactcg
      481 gtcagcacgc ggtacagccc cggccggcga tcgcggaaga accccatgct ggtgcgcagg
      541 cgtcgaatct gcgtgaggtc gagccgggcc agcgcgatgc cggcgtcgct ccggccgagc
      601 tcggcgagct tgtcgccacg cgcgtcggcg acgaacgagc ttccgtagaa ggtgatggcg
      661 ccggcgccat cgccctcggt gccgatccgg ttggccgcga ccacgccgac cgcgttggcg
      721 acggcgtgac cgatcatcac gcgctgccag gcgtcgcggg tgtcgagctc gggctcctcg
      781 ggctccgagc cgatcgccgt cggatagaac aggacgtcgg cgccggcgag cgtcatcgcg
      841 cgcgcggcct cgggaaacca ctgatcccag cagatcgcga cgccgatcgt gccgaaccgg
      901 gtcgcgaacg cgcggaaccc ggtgttgccg ggcttgaaga agaacttctc ctggtagccg
      961 gggccgtcgg ggatgtggct cttgcggtag acgccgagcg agcggccgtc ggcgtcgagc
     1021 acggcgatcg agttgtaata ctccgggccg gcctgctcga agaacgatac ggggatcacg
     1081 acgccgagct cggcggcgag ctgctggaag tgacggagcg tcgggtggcc ctcggcgggc
     1141 cgcgcacggg agaagtcggc ctcgcgctgg tcgcggcaga agtagtggcc ctcgaacagc
     1201 tcgctcggca ggacgatctg cgcgccgcgg ccggccgcct cgcggcacag cgcggtcacc
     1261 cgggcgacgt tgccggcgac gtcgtcggtc agcgcggtct gcagggcggc gacggtgact
     1321 tccatgccgc gcactgtacc cgatcgggta ccgccgaccg gatcatccga tcgccggcgg
     1381 gacccgcggc tgctgctggg tcgagcagtg gaacgcgccg ccgccgacga cgaccggctt
     1441 gccgggaacg ccgatcacgc gccgggtcgg aaacatcgcc tcgatcgcct tcaccgcggc
     1501 gtggtcggca cggaccccgt aggtcggcac caccaccacg gtgttggcga tgtagaagtt
     1561 catgtagctc gccggcatca ggttgccggc ggcgtcctcg accgcgcccg gcgacggcac
     1621 cgtcacgacc tcgagccggt cgccgccggc ggtccgcgcc gcgccgaggt cggcgaggat
     1681 gccggcgagc gcgtcgcggt tcgggtcgcc ggcggcgggc tccatgcacg cgaccacgcc
     1741 gggcgcgacg aaccgcgcga gcgtgtcgat gtgaccgtcg gtgtgatcgt tggcgaggcc
     1801 gcgatcgagc cagaccacct tgtcgaccgc gagcgccc
//
LOCUS       DASVOR010001369         5294 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_422484,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001369 DASVOR010000000
VERSION     DASVOR010001369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5294
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422484"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VF516_26060"
     CDS             <1..690
                     /locus_tag="VF516_26060"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8111231.1"
                     /translation="GSLRMGVIPTVAPYWLPALLPEVHRRFPRLELILREDQTSRLLA
                     LLSAGQLEIALLAIPVPGDFTTAPVAREGFVAAAPRGASLIKHRGKLTERDLEAETVL
                     LLEDGHCLRDQALEVCKRAGAVESVEVRATSLPTLVQMVAGGLGVTLLPEASVPTLVQ
                     RHGPVALAEFAKPVPGRNIGLAWRTSSGRLREFRLLAETMSASAEDFLARSRENTRKL
                     SEPGGGRPRKS"
     gene            complement(1086..4925)
                     /locus_tag="VF516_26065"
     CDS             complement(1086..4925)
                     /locus_tag="VF516_26065"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111232.1"
                     /translation="MTASLDLDHPWPGPDSFRTQDAAFFRGRDSEIKQLLQLVRRERS
                     VVLYGASGLGKTSLINAGLIPRLLRTQGKNQLLVAATDLNVSLTAELKNNNASDNEIA
                     ILAKSPFDLVTNAPGDKLAPGADEYFAVPIRISYGTGAPSVSEQLQTEILSSRVGPGM
                     PQELPHRTAWELLHLRDQPIEGAQPLLIFDQFEELFTIGAGTPQATELIEELKALIEG
                     VPPASVRERLERHPEDARSLSFQRRGHRVLISIREDFLYGLESLRAQLPSIIHNRYRI
                     GPLGGAMALEVVLQRPVAAQAAPGAEAAAAPLVEPDVAELIVRTVAAATDDRPLEALE
                     VEPALLSILCSELARRRSPGTPITRELVTGSRADIISSFYERALHGAPDAVRAYLEDQ
                     LVTAMGFRTSAVVDEALAIPGFTSELLSDLVNKRLLRVIERTKGRWIELTHDILTEIA
                     AHSRALRRERQRVEVERLARARNQARRIRAALALGGMVFAFAVLASLYWAALTSRKAE
                     EFQRDAVMRNYETERRSRQAADEARRALRRQAIDASLREGSYREALAQLAAVVREEAG
                     AGWARALVGDLLSRRTWPVPASPMFPEGPFAYLACSRSGTRCAAAYRDGRVLVRGDLS
                     RDLATDQTGFGTLAMSEDGTGLVFVPDEPGTAIRWMIGIADPAPFRFPYNDTWNVWDA
                     SSDTRVVVLPSGPDLTIWRFDAPAPTSTTIHRDLVGAPFALSPDGTWLTYWEHKGYRG
                     SIALANAHGQELRSVPASGDVVEMKIDPSSKALLVALADGTVRRWTIPDLQQLTVSRT
                     SRRIQGIGFDWTANQCLLALEGGGVELWSEPWLAPHVLTRASHGVTSIFHSFAPDDQW
                     LAVATRDGTVSTWSSSGEGFGEPVHLTGVAIAAALNGRSLLAVSLGGTSARWTIPTPS
                     HPRQYTLGESVWSTWFPSEDTFVAYGGSKALALHGDTTKTMQLRRHNLFLSRDQRHMV
                     TLYTNGLFLREEMPSGENKDDADKLLIPGIIDDVAFSADGKRLAASGGGKGYLFDVTS
                     GQPIGKQIDHVSKMWLNEDGTILATKMNDVGLMLWQCASDGSLGKLADVDVLDVSSVA
                     FDHANDAIVMAIEHQARIWSLRDRTFTGRAVSHGGPITAVAFSPDGRWIVTASADKRA
                     RIWEAASGLPASDWFEHDGSVSAVDFSPSGRKLLTGSSDGSVRIWDLASGEDATEQER
                     RWLARTAEILSGLHIDPGTGDAVPETRAFEALEALRRDIERACPDATRPGCASAASDL
                     IRKVLGKRDAAPLPTAP"
     gene            complement(4922..>5294)
                     /locus_tag="VF516_26070"
     CDS             complement(4922..>5294)
                     /locus_tag="VF516_26070"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8111233.1"
                     /translation="RLEREAGVRVWLDRDALRGGDQWERHIADTLRGCALCVPVISAE
                     ARSGGFRYLRAEWRQAVKLQAGRPADQTFLLPLVIDDTRPDDPAIDPELRALHWRRLD
                     DEADMQRFLTEVRAAVEKSLS"
BASE COUNT          804 a         1821 c         1804 g          865 t
ORIGIN      
        1 ggctcgttgc gcatgggggt catcccgacg gtcgctccgt actggctgcc cgccctgctc
       61 cccgaagtcc accgccggtt cccgcggctc gagctgatcc tgcgcgagga ccagacctcg
      121 cggctgctcg cgctgctgtc cgcaggccag ctcgagatcg ccctgctggc gatcccggtc
      181 ccgggcgact tcaccaccgc gccggtggct cgtgaaggct tcgtcgcagc agcgccgcgc
      241 ggcgcgtcgc tgatcaagca ccgcggcaag ctcaccgagc gcgacctcga ggccgagacc
      301 gtgctcttgc tcgaggacgg ccactgcctg cgcgaccaag cgctcgaggt ctgcaagcgc
      361 gccggggcgg tcgagtcggt cgaggtccgc gccaccagcc tgccgacgct ggtccagatg
      421 gtcgccggcg ggctcggcgt cacgctgctc cccgaggcgt cggtcccgac cctggtccag
      481 cgccacgggc cggtcgcgct cgccgagttc gccaagccgg tgcccgggcg caacatcggc
      541 ctggcgtggc gaacctcgtc gggccggctc cgcgagttcc ggctgctcgc cgagaccatg
      601 agcgcgtcgg ccgaggactt cctggcccgc tcgcgcgaga acacgcgcaa gctgtccgag
      661 ccggggggcg gccggccgcg caagagctga gcagacctca tcgggcgcgc ccgggattcg
      721 tgcgaccgac tcagcgaatc gctgatcgcc ggacgcaagg gccacaacgc cagcgacgcc
      781 aggattgccg tcgtcgcgag agtccgtcga gctggtcggc aatccgctcg gcgacgcagt
      841 cacgcgcgag aaggcctgcg agcagtgggt ggagcttgag gcggcgagtc ctgcgggtcc
      901 cggtcaatca ccgggtgcgc gtcgggtacg cgatcgtgtg gcgaagcaat cctggcgcgg
      961 ttcctggcgc gatcgtggga gatcgctcgg cgtccgcttg ccgagtacag tgcggatgag
     1021 atcgcgcgtc gccgcgacgg attgctcgga gcactcccac gccggaagaa gatgtccgca
     1081 ggggttcacg gcgcggtcgg caatggggcg gcatcgcgct tgccaagcac tttgcggatg
     1141 aggtcgctcg ccgccgaggc gcacccgggc cgggtcgcat ccgggcacgc tcgctcgatg
     1201 tcgcggcgca gcgcttccag cgcctcgaac gccctggtct cgggaacggc atcgccggtg
     1261 cccggatcga tgtgcagccc ggacaagatc tctgcggtcc gtgcgagcca gcgccgctcc
     1321 tgttcggtcg cgtcctcgcc gctcgctagg tcccagattc gaacggatcc gtcgctcgag
     1381 ccggtgagca gcttgcgccc tgacggcgag aagtccaccg cggagacgct cccatcatgc
     1441 tcgaaccagt cgcttgccgg cagcccgctc gctgcctccc agatccgggc tcgcttatcg
     1501 gccgaggcgg tcacgatcca gcgaccgtcg gggctgaacg cgacggcggt gatcggaccc
     1561 ccgtgagaca cggcgcggcc tgtgaacgtc cggtcgcgta ggctccagat gcgggcctgg
     1621 tgctcgatgg ccatcacgat cgcatcgttc gcatgatcga acgccacact ggacacatcc
     1681 agcacatcaa cgtcggccag cttgccgagc gagccatcgc tcgcacactg ccagagcatc
     1741 aaccccacgt cgttcatctt cgttgcgagg atcgtgccat cttcgttgag ccacatcttc
     1801 gagacatggt cgatctgttt gccgattggc tgtccggaag tgacatcgaa cagatacccc
     1861 ttgccgccac ccgacgcagc caggcgcttg ccgtcggcgc taaacgcgac atcgtcaatt
     1921 atcccgggta tgagcagctt gtccgcatca tccttatttt ctccggacgg catttcctct
     1981 ctcagaaaca gaccatttgt gtataatgtc accatatgac gctgatctct cgagagaaat
     2041 aggttgtgcc gtcgcagttg cattgttttg gtggtgtctc cgtgcagcgc caatgctttc
     2101 gatcctccat aggcaacgaa cgtgtcctcg gatggaaacc acgtgctcca gacggactca
     2161 cctaacgtgt actgccgggg atggctcggc gtcgggattg tccagcgagc actggttccg
     2221 cccagggaga cagccagcaa actgcggcca ttcagggcag ctgcgatggc cacgcccgtg
     2281 aggtgcactg gctccccgaa gccctcgccg gaggaactcc atgtgctcac cgttccatcg
     2341 cgcgtggcaa ccgctagcca ctggtcatcg ggcgcaaaac tgtggaagat gctcgtaaca
     2401 ccgtggctcg cccgcgtcag cacgtgtggc gcaagccacg gctcgctcca cagctccacg
     2461 ccgccgcctt cgagcgccag gaggcattgg ttagctgtcc agtcgaaccc gatcccctgg
     2521 atcctgcggc ttgttcggga caccgtgagc tgctggaggt ccgggatggt ccagcgtcgc
     2581 actgttccgt cagccaaggc gacgagcagc gccttcgagc tggggtcgat cttcatctcc
     2641 acgacatcgc cagatgccgg gacgctacga agctcttggc cgtgcgcgtt ggcgagcgcg
     2701 atcgaacccc tataaccctt atgctcccag tacgtcagcc atgttccgtc ggggctcaga
     2761 gcaaagggag ctcccacgag gtcacggtgg atcgtcgtag aggttggcgc gggtgcatcg
     2821 aaccgccata tcgtgagatc gggcccgctt ggcaagacca ccacccgggt atcggagctc
     2881 gcgtcccaga cgttccatgt gtcgttgtac ggaaaccgaa acggcgccgg atccgcgata
     2941 ccgatcatcc atcgaatggc ggtgccaggt tcgtccggaa cgaacacgag accggtgccg
     3001 tcctcgctca tggcgagcgt gccaaagcct gtttgatccg tggccagatc gcgcgagagg
     3061 tctccgcgca cgagcaccct tccatcccgg taggccgcgg cgcatcgagt gccgcttcgg
     3121 ctgcaagcca ggtaggcaaa gggaccctcc gggaacatcg gcgatgccgg caccggccag
     3181 gtcctccgcg ataggaggtc ccctaccagc gcgcgtgccc agccggcgcc cgcctcttcc
     3241 cgcacgacag ctgcgagctg tgccagggcc tcgcggtagc tgccctcgcg caggctcgcg
     3301 tcgatcgcct gtcggcggag agcacgcctt gcttcatcgg cggcctggcg cgatcgccgc
     3361 tccgtctcgt agttgcgcat gacggcgtcg cgctgaaact cctccgcctt gcgcgaggtc
     3421 agcgcggccc agtagaggct tgcgagcacg gcgaacgcga acaccatgcc gcccagcgcc
     3481 agtgccgcgc ggattctccg tgcctggttt cgggcgcggg cgagccgctc cacctccacg
     3541 cgctgccgct cgcgtcgcaa agcgcggctg tgggcggcga tctcggtgag gatgtcgtgc
     3601 gtgagctcga tccacctgcc cttggtccgc tcgatcacgc gcagcaggcg cttgttgacg
     3661 aggtcggaca ggagctcgct cgtgaatccc gggatggcca gggcctcgtc gaccacggcg
     3721 ctggtgcgga accccatcgc cgtgaccagc tggtcctcga ggtaggctcg cacggcgtcc
     3781 ggtgcgccgt gcagggcgcg ctcgtagaat gacgagatga tgtcggcccg gctgccggtc
     3841 accagctcgc gcgtgatcgg cgtgcccggg gagcgccgcc gcgccagctc gctgcatagg
     3901 atgctgagca gcgccggctc gacctcgagc gcctcgagtg gccggtcgtc ggtcgccgcc
     3961 gccaccgtcc ggacgatcag ctccgcgacg tccggctcga cgagcggcgc agcggccgcc
     4021 tcggcccccg gggctgcttg cgcagcgacc ggccgctgca ggacaacctc gagcgccatg
     4081 gcaccgccga gcggaccgat ccggtagcgg ttgtggatga tcgacggcag ctgggcgcgg
     4141 agtgactcga gcccgtacag gaagtcctcg cggatgctga tcaggacgcg gtggccgcgg
     4201 cgctggaacg acagcgaccg agcgtcttcg gggtggcgct ccagccgctc gcgcacgctt
     4261 gccggcggga ctccctcgat cagcgccttg agctcctcga tcagctcggt ggcctgcggg
     4321 gtccctgcgc cgatcgtgaa cagctcctcg aactgatcga agatcaggag cggctgcgcg
     4381 ccctcgatcg gctgatcgcg caggtgcagg agctcccacg ccgtccggtg cgggagctcc
     4441 tgcggcatcc cggggccgac gcggctgctc aggatctcgg tctgcagctg ctcggacacc
     4501 gagggcgcgc cggtgccgta gctgatccgg atcggcaccg cgaagtactc gtcggcgccg
     4561 ggcgcaagct tgtcccctgg ggcgttggtg accaggtcga acgggctctt ggcgaggatg
     4621 gcgatctcgt tgtcgctcgc gttgttgttc ttgagctcgg cggtcagcga gacgttgagg
     4681 tcggtcgccg cgacgagcag ctggttcttg ccctgggtac gcagcagccg cgggatcagg
     4741 cccgcgttga tcagcgaggt cttgccgagc cccgatgcgc catagaggac caccgagcgc
     4801 tcgcgacgga cgagctggag cagctgcttg atctcgctgt cgcggccgcg gaagaatgcg
     4861 gcgtcctggg tacggaacga atcgggcccc ggccacgggt gatcgaggtc gagcgacgcg
     4921 gtcacgagag cgacttctcg acggccgcgc ggacctcggt gaggaagcgc tgcatgtcgg
     4981 cctcgtcgtc cagccggcgc cagtggaggg cccggagctc ggggtcgatc gccggatcat
     5041 ccggccgggt gtcgtcgatg accagcggga gcaggaacgt ctgatcggcg ggccggccgg
     5101 cttgcagctt cacggcttgc cgccactcgg cgcgcaggta gcggaagccg ccggagcggg
     5161 cctcggccga gatcaccggg acgcacagcg cgcagccgcg cagcgtgtcg gcgatgtggc
     5221 gctcccactg gtcgccgccg cgcagcgcgt cccggtcgag ccacacacgc acgccggcct
     5281 cgcgctcgag ccgc
//
LOCUS       DASVOR010001370         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_364739,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001370 DASVOR010000000
VERSION     DASVOR010001370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364739"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VF516_26075"
     CDS             complement(<1..912)
                     /locus_tag="VF516_26075"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HEX8111234.1"
                     /translation="MLDVRYNDRYHRQSLITWWDQARVARSRILVIGAGALGNEILKL
                     LSLIGVGATLVYDPDRIERSNLSRSVLFREVDEGHPKAEVAVRRMAELNPDARGHARC
                     DNAIATAGLGVFLWADVVIGAVDNREARVFINSACARTRRTWVDGAIEGLSGIVRAFT
                     PHSGACYECTMNATDRKLLAERRSCAMLAREAVLRGHVPTSAVAASVIGAMEVQEAIK
                     LVHGQPALVGQGLHLDGLFGEISRVAYPRRADCPGHDDLGAIAPLGLGVADITLGELL
                     DRADAAVGPGASLDLSRDVIVQWSCPSC"
     gene            complement(952..1257)
                     /locus_tag="VF516_26080"
     CDS             complement(952..1257)
                     /locus_tag="VF516_26080"
                     /inference="COORDINATES: protein motif:HMM:NF020396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EsaB/YukD family protein"
                     /protein_id="HEX8111235.1"
                     /translation="MGDINLEVWDATGNKRLHVQVPDDVAVERILMVLADKLHLPRYA
                     PDGQLMSYKFHHRRLGIQLLDDQTLLQQQVQDSDIVRIQPEITAGATTTRRSPRHGG"
     gene            complement(1459..>1782)
                     /locus_tag="VF516_26085"
     CDS             complement(1459..>1782)
                     /locus_tag="VF516_26085"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8111236.1"
                     /translation="DMRAHLPRFAGENGERNQRLVAALDRIAGARGVRPSQLAIAWVL
                     AKGSSSVPVIGARTRRQLDESLAALAIQLTPGELAEIEQAVPASEVAGTRYAGPQMQM
                     LDSER"
BASE COUNT          261 a          636 c          619 g          266 t
ORIGIN      
        1 gcacgacggg cagctccact gcacgatcac gtcgcgcgac aggtcgagcg acgcgcccgg
       61 gccgaccgcg gcgtccgcgc gatcgagcag ctcgccgagc gtgatgtcgg cgacgccgag
      121 gccgagcggc gcgatcgcgc cgaggtcgtc gtggccggga cagtcggcgc gccgcggata
      181 cgcgacccgg ctgatctcgc cgaacagccc gtcgaggtgc agcccctggc cgaccagcgc
      241 gggctggccg tgcacgagct tgatcgcctc ctggacctcc atcgcgccga tcaccgacgc
      301 ggccaccgcg gaggtcggga cgtggccgcg cagcacggcc tcgcgcgcga gcatcgcgca
      361 ggaccggcgc tcggcgagca gcttgcgatc ggtggcgttc atcgtgcact cgtagcaggc
      421 gccgctgtgc ggcgtgaacg cgcggacgat gccagataat ccctcgatcg cgccgtcgac
      481 ccaggtccgc cgcgtgcgcg cgcacgccga gttgatgaac acccgcgcct cgcggttgtc
      541 gaccgcgccg atcacgacgt cggcccacag gaacacgccg agcccggcgg tcgcgatcgc
      601 gttgtcgcac cgcgcgtggc cgcgggcgtc cgggttgagc tccgccatcc ggcgcaccgc
      661 gacctcggcc ttggggtggc cctcgtcgac ctcgcggaac agcacgctgc gcgacaggtt
      721 cgagcgctcg atgcgatccg gatcgtagac cagcgtggcg ccgacgccga tcagcgacag
      781 cagcttgagg atctcgttgc cgagcgcgcc ggcgccgatc acgaggatcc ggctgcgcgc
      841 gacgcgcgcc tgatcccacc aggtgatcag cgattgccgg tgataacgat cgttgtagcg
      901 aacgtcgagc aacttccctc cttccaccgc gatctcacta ccgccgcccg gctatccgcc
      961 gtgccttgga gatcgccgag ttgtggtagc gcccgcggtg atctccggct ggatccggac
     1021 gatgtcggag tcctgcacct gctgctggag cagggtctgg tcgtcgagca gctggatccc
     1081 gagccggcgg tgatggaact tgtagctcat cagctgaccg tccggcgcat agcgcggcag
     1141 gtgcagcttg tcggcgagca ccatcaggat ccgctcgacg gcaacatcgt cgggcacctg
     1201 cacgtggagg cgcttgttgc cggttgcatc ccacacttcc agattgatgt cacccacgac
     1261 gcagcctcct gtcttttcat atgccgagca gccgaaatat gcaacagccc gcgacctatg
     1321 aggtttgtcc agcgcgggcg tgtcggtcga tgagcctgga ctcccgcggt catcaccgcg
     1381 accggtacag gctgcgacag gctcgtgcgc tcggtggtct ggccagcaga ccgcgctccg
     1441 ggccgtcact ggcagctact aacgctcgct atcgagcatc tgcatctgcg ggcccgcgta
     1501 gcgcgtgccg gcgacctcgg acgccggcac cgcctgctcg atctcggcga gctcgccggg
     1561 cgtgagctgg atcgccagcg cggcgagcga ctcgtcgagc tggcgccggg tgcgcgcgcc
     1621 gatcaccggg acgctcgacg agcccttggc gagcacccag gcgatcgcga gctgagacgg
     1681 ccggacgccg cgcgcgccgg cgatccggtc cagcgcggcg accaggcgct ggttgcgctc
     1741 gccgttctcg ccggcaaacc gcggcaggtg ggcgcgcatg tc
//
LOCUS       DASVOR010001371         5750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_335318,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001371 DASVOR010000000
VERSION     DASVOR010001371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5750
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335318"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VF516_26090"
     CDS             <1..300
                     /locus_tag="VF516_26090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111237.1"
                     /translation="PVGSRLELGLDAVFVGYSVIPSVRVRLAGDALAVHAVGAVPISF
                     NDGSTMERFVAGGFGLGVRYQPAPSFAMRLESYAAYAGKAHGWTIPMFLGGELWF"
     gene            291..1565
                     /locus_tag="VF516_26095"
     CDS             291..1565
                     /locus_tag="VF516_26095"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HEX8111238.1"
                     /translation="MVLIRLGWLCVLACAACSQSLFDAYGPPGSGPATCKAPCIADAA
                     VDFDGSATGKNQMWRYLDDHRDRTWTAMTSSKTTMTGADPANHLTTCAANPGAAACAM
                     LSDALLLSSSGATSAADPAIEFTAPAQQVIQLTVKAFMASGSDQTIRLYRNGRPDALF
                     TGVATAGTPLEQTITVDALAKDRFLVALAPMAAGASDVAIQFIASSAGMLFPQTCLLA
                     VEFARATGNLVSDLCQSVSFTHYDAAFAAIPVALGDDPFGQKNTAADIALGTDFETTQ
                     PIAQNTTLTLQLWARQRTMPTEDVWLASDMDLTDNAPGGLALGISRFGMLDAQTATVS
                     NPTTIDVADALTPWPGTGDWHFVRVVQSGGNLNLCLDGKQKASTPVPDGHLRTVEPLF
                     LGRNKFGIPAGESFFDGFLDDVRIFKGALPCE"
     gene            1606..2742
                     /locus_tag="VF516_26100"
     CDS             1606..2742
                     /locus_tag="VF516_26100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyquinol 1,2-dioxygenase"
                     /protein_id="HEX8111239.1"
                     /translation="MRRSRTGWEDISRVRLRQPAARLCVALRRDEIVIRAGAAFSGFS
                     PPGGGPRVDAMATTAANAPIRRHHHITLCSGTAQEDYDFHTKVLSLKSVKKTALYDGD
                     VPIYHLYYGNDLGEESTLVTTFPMRQSGRKGRKGTGQIRTLSLSIPVSGLGFWHKRLT
                     DHGFAVREASRFGERALAFQHPCGLDYELVGITDDNRKPYSNGEVPSELAIRGTHAIV
                     VSVREHETSNEFMTVGWSARRIAEDGRYVRYVFGDGESPHTGKIVDFALEPELPQASW
                     TYGEGIVHHCAFQVDDYTAQDQVKFRIEGMGFTDVSERKDRGYFDSIYVRTPAGALFE
                     ATVSKPGGMTVDEKIDQIGTSFQIPPPFRHRAKELMEYLEPLRY"
     gene            complement(2760..3161)
                     /locus_tag="VF516_26105"
     CDS             complement(2760..3161)
                     /locus_tag="VF516_26105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111240.1"
                     /translation="MSPTTNLAMTLVLLVAACGTDTADPPPTYSELYTRYFAPGTPGH
                     CAMAGCHGDPAHLIWLCGTTKDTCYAGMTSPDAGLINTAVPRLSLIADPRNSPLSWIN
                     PNGPMPNDKPGPFPEGRDAILAWIIAGAPND"
     gene            3385..5364
                     /locus_tag="VF516_26110"
     CDS             3385..5364
                     /locus_tag="VF516_26110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEX8111241.1"
                     /translation="MQWTRPVFAAIVLGGTAASLAAQPAKPPAPPARPAPRYPALPSE
                     TPAQFTPSTYGFEYTWREVMIPMRDGVKLFTVILVPRGARRAPMLLTRTPYNASALTA
                     HGHSGHLAPILQGYDNATDVIVEGGYIRVVQDVRGKYKSEGDYVMNRPVHGPQNPTPV
                     DHATDTYDTIDYLVKHVPESNGRVGILGISYDGFLPLMALVHPHPALKVSVPMNPMVD
                     GWRGDDWFHNGAFRELGIDYIHDQQATRTSEEKFWRSHFDDYDTFLEAGSAGELGRRH
                     AMEQIGFWNKIVAHPAYDAFWRDQAVDQVLAKEPLAVPVMLVHSLWDQEDIYGAIAVY
                     KAIAPKDTNHDKVFLVLGPWHHGGAIGDGSSLGPIRFDADTALQFRRDILRPFLDHYL
                     KDGEPKADIAPVHAFETGTNHWQRLASWPAGCASGCAIKPTALYLAEGGKAAFTAPPA
                     GGAGFDEYVSDPAKPVPFRRRPIEPPSYQDNIAWQNWLITDQREASGRPDVLSFTTDV
                     LTAPVKISGQPAVHLIASTSGTDSDWVVKVIDVYPDEVASDPTMGGYQLMISADIFRG
                     RYREGLDTARPIAADKPLLYRFDLPTANHVFLPGHRIMAQIQSSWFPLYDRNPQTFVP
                     NIFWAKPGDYRKATQRVYHAPDQASFVELPVVSAP"
     gene            complement(5372..5578)
                     /locus_tag="VF516_26115"
     CDS             complement(5372..5578)
                     /locus_tag="VF516_26115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HEX8111242.1"
                     /translation="MANADKRKQSLYFPEDMLKEIQHEAHRLDRSLSWIVQQGWKIAR
                     DKLKAFPASNDDPEGLPDPREEST"
BASE COUNT          913 a         2035 c         1933 g          869 t
ORIGIN      
        1 ccggtcggct cgcggctcga gctcgggctc gacgccgtgt tcgtcggcta ctcggtgatc
       61 ccgtcggtcc gggtccggct ggccggcgat gcgctcgcgg tccacgccgt cggggccgtg
      121 ccgatctcgt tcaacgacgg atcgaccatg gagcgcttcg tcgccggcgg gttcggcctg
      181 ggcgtgcgct accagccggc gccgagcttc gcgatgcggc tcgagagcta tgccgcctac
      241 gccggcaagg cgcacggctg gacgatcccg atgttcctcg gaggcgagct atggttctga
      301 tccgactcgg ctggctctgc gtcctggcct gcgctgcgtg cagccagtcg ctgttcgatg
      361 cctacggccc gcccggtagc gggccggcga cctgcaaggc cccctgcatc gccgatgcgg
      421 cggtcgactt cgacggcagc gcgaccggca agaaccagat gtggcgatac ctcgacgacc
      481 atcgcgaccg cacgtggacg gcgatgacat cgagcaagac gacgatgacc ggcgccgatc
      541 cggccaacca cctcacgacc tgcgcggcga accccggggc ggcggcctgc gcgatgctct
      601 cggatgcgct gttgctatcg tcgtccggcg cgaccagtgc ggccgatccc gcgatcgagt
      661 tcaccgcccc tgcccagcag gtgatccagc tgaccgtgaa ggcgttcatg gcgagcggct
      721 ccgaccagac gatccggctg tatcgcaacg gccggccgga cgccctgttc accggcgtcg
      781 ccaccgccgg cacgccgctc gagcagacga tcacggtcga cgcgctggcc aaggaccgct
      841 tcctggtcgc gctggcgccg atggccgccg gcgccagcga cgtggcgatc cagttcatcg
      901 cgagctcggc cgggatgctg ttcccgcaga cgtgcctgct cgccgtggaa tttgcacgcg
      961 ccaccggcaa cctggtgagc gatctgtgcc agagcgtctc cttcacccat tacgacgccg
     1021 cgttcgcggc gatcccggtg gcgctcggcg acgatccctt cgggcagaag aacaccgccg
     1081 ccgacattgc cctcggaacc gacttcgaga ccacccagcc gatcgcccag aacaccacgc
     1141 tcaccctgca gctgtgggcg cggcagcgca cgatgccaac cgaggacgtc tggctggcct
     1201 ccgacatgga tctcaccgac aacgcgcccg gcggcctggc cctcgggatc tcccggttcg
     1261 ggatgctcga cgcgcagacg gccacggtgt cgaacccgac gacgatcgac gtcgccgacg
     1321 cgctcacgcc gtggcccggc acgggagact ggcacttcgt tcgcgtggtc cagagcggcg
     1381 gcaacctcaa cctgtgtctc gacggcaagc agaaggccag cactcccgtc cccgacgggc
     1441 acctcaggac ggtcgagccg ctgttcctgg gcaggaacaa gttcgggatc ccggccggcg
     1501 agtcgttctt cgacggcttc ctcgacgacg tgcggatctt caagggcgcc ctgccgtgcg
     1561 aatgacgtag ccgggcgagc cgagcccagg atgcggtgcc gcgcgatgcg gcgttctcgc
     1621 accgggtggg aggatatcag ccgggtccgg ctgcgtcagc cggcggcgcg gctgtgcgtc
     1681 gcgctacggc gcgacgagat cgtgatacgc gccggggcgg cgttttccgg cttctcgccg
     1741 cccggcggcg ggcctagagt cgacgccatg gccaccaccg ccgccaatgc cccgatccgg
     1801 cgtcaccacc acatcacgct gtgctccggt accgcccagg aagattacga cttccacacc
     1861 aaggtgctgt cgctcaagag cgtcaagaag accgcgctct acgacggcga cgtcccgatc
     1921 tatcacctgt attacggcaa cgacctcggc gaggagagca cgctggtcac cacgttcccg
     1981 atgcggcagt ccggccgcaa gggccgcaag ggcaccggcc agatccgcac gctcagcctg
     2041 tcgatcccgg tgtcgggact ggggttctgg cacaagcggc tgaccgatca cggcttcgcg
     2101 gtccgcgagg cgtcgcggtt cggcgagcgc gcgctcgcgt tccagcaccc gtgcgggctg
     2161 gactacgagc tggtcgggat caccgacgac aaccgcaagc cgtactccaa cggcgaggtc
     2221 ccgagcgagc tcgcgatccg cggcacccac gcgatcgtgg tcagcgtgcg cgagcacgag
     2281 acctcgaacg agttcatgac cgtgggctgg agcgcgcggc ggatcgccga ggacggccgc
     2341 tacgtgcgct acgtgtttgg cgacggcgag agtccgcaca ccggcaagat cgtcgacttc
     2401 gcgctcgagc ccgagctgcc ccaggcgagc tggacctacg gcgagggcat cgtccaccac
     2461 tgcgcgttcc aggtcgacga ctacaccgcg caggatcagg tcaagttccg gatcgagggc
     2521 atgggcttca ccgacgtgtc cgagcgcaag gaccgcggct acttcgactc gatctacgtc
     2581 cgcaccccgg cgggcgcgct gttcgaggcc acggtgtcca agccgggcgg gatgacggtc
     2641 gacgagaaga tcgatcagat cggcaccagc ttccagatcc cgccgccgtt ccggcaccgc
     2701 gccaaggagc tgatggagta cctcgagccg ctgcgctact agcggctccc agcagcgcgc
     2761 tagtcgttcg gggcgcccgc gatgatccac gccaggatcg cgtcgcggcc ctcggggaac
     2821 ggccccggct tgtcgttggg catcggcccg ttcgggttga tccagctgag cggcgagttc
     2881 cgcggatcgg cgatcagcga cagcctgggc accgcggtgt tgatcagccc cgcgtcgggg
     2941 gacgtcatcc ctgcgtagca ggtgtccttg gtggtgccgc acagccagat gaggtgcgcg
     3001 ggatcgccgt ggcagccggc catggcgcag tggcccggcg ttccgggcgc gaagtaccgg
     3061 gtgtacagct cgctgtaggt cggcggcgga tcggcggtgt cggtgccgca cgcggcgacg
     3121 agcaacacca gggtcatcgc gaggttcgtg gtcggcgaca tgacggctcg gggctggcgg
     3181 agtctatcta catcgaactc gaggccgcat ccggccccgg gtcgcggtcc gtaacacgtg
     3241 ttcgcacatc gccgcctgcc tgtgcgcggc ggcccatgtg ggtcgacgtg ggcgagccag
     3301 atgtgcaggc ccgcacatcc ggaccggcgg tcgggccggg aatcagtatc ctgccgcccc
     3361 acacgatgcg atgaagaggt ccccatgcag tggacccgac cggttttcgc agcgatcgtg
     3421 ctcggcggca ccgccgcctc gctggcggcg caacccgcca agcccccggc gccgcccgcc
     3481 aggcccgcgc cgcgctatcc cgcgctgccg agcgagacgc cggcgcagtt cacgccgtcg
     3541 acctacggct tcgagtacac gtggcgcgag gtgatgatcc cgatgcgcga cggcgtgaag
     3601 ctgttcaccg tcatcctggt gcccaggggc gccaggcgcg cgccgatgct gctcacccgc
     3661 acgccgtaca acgcgtcggc gctgaccgcg cacggccact ccggccacct cgcgccgatc
     3721 ctgcagggct acgacaacgc gaccgacgtg atcgtcgagg gcggctacat ccgcgtcgtc
     3781 caggacgtgc gcggcaagta caagtccgag ggcgactacg tgatgaaccg gccggtgcac
     3841 gggccgcaga acccgactcc ggtcgatcac gccaccgaca cctacgacac gatcgactac
     3901 ctggtgaagc acgtcccgga gagcaacggc cgggtcggta tcctcgggat ctcgtacgat
     3961 ggcttcctgc cgctgatggc gctggtccac ccccacccgg cgctcaaggt ctcggtgccg
     4021 atgaacccga tggtcgacgg ctggcgcggc gacgactggt tccacaacgg ggcgttccgc
     4081 gagctcggga tcgactacat ccacgatcag caggcgacgc gcacctccga ggagaagttc
     4141 tggcgcagcc acttcgacga ctacgacacg ttcctcgagg ccggctcggc cggcgagctc
     4201 ggccgccgcc acgccatgga gcagatcggg ttctggaaca agatcgtcgc gcaccccgcg
     4261 tacgacgcgt tctggcgcga ccaggcggtc gaccaggtgc tcgccaagga gccgctcgcc
     4321 gtcccggtga tgctggtcca cagcctgtgg gaccaggagg acatctacgg cgcgatcgcg
     4381 gtgtacaagg cgatcgcgcc caaggacacc aaccacgaca aggtgttcct ggtgctcggc
     4441 ccgtggcacc acggcggcgc gatcggcgac ggcagctcgc tcggcccgat ccggttcgac
     4501 gccgacaccg cgctgcagtt ccgccgcgac atcctgcggc cgttcctcga ccactacctc
     4561 aaggacggcg agccgaaggc cgacatcgcg ccggtccacg cgttcgagac cgggaccaac
     4621 cactggcagc gcctcgccag ctggcccgcc gggtgcgcca gcggctgcgc gatcaagccg
     4681 accgcgctct acctcgccga gggcggcaag gcggcgttca ccgcgccgcc ggcgggcgga
     4741 gcggggttcg acgagtacgt gtccgacccg gccaagccgg tgccgttccg gcggcggccg
     4801 atcgagccgc cgagctacca ggacaacatc gcctggcaga actggctgat caccgaccag
     4861 cgcgaggcct cgggccgccc cgacgtgctg agcttcacca ccgacgtgct gaccgcaccg
     4921 gtcaagatct ccggacagcc ggccgttcac ctgatcgcat cgaccagcgg caccgactcg
     4981 gactgggtgg tcaaggtgat cgacgtctac cccgacgagg tcgcgagcga tccgacgatg
     5041 ggcggctacc agctgatgat ctcggccgac atcttccgcg gccgctatcg cgagggactg
     5101 gacaccgccc ggccgatcgc cgcggacaag cccctgctct atcgcttcga cttaccgact
     5161 gccaaccacg ttttcctacc tggccaccgg atcatggccc agatccagtc gagctggttt
     5221 cccctctacg atcgcaaccc gcagaccttc gtcccgaaca tcttctgggc caagccagga
     5281 gactaccgca aggcgaccca gcgcgtgtat cacgcgccgg atcaggcgag cttcgtcgag
     5341 ctgcccgtcg tgtcggcgcc gtagccgccg gctaggtcga ttcctcgcgc gggtcgggca
     5401 ggccctcggg atcgtcgttg ctcgccggga atgccttgag cttgtcgcgg gcgatcttcc
     5461 atccctgctg gacgatccag gacagagaac ggtcgaggcg atgagcctcg tgctgaattt
     5521 ctttgagcat gtcctcgggg aaatacaagg actgctttct cttgtcggcg ttggccatct
     5581 cgctccgttc ggggctatcg aagtgtggcg ccgattctat cccgagccat ggcgctggac
     5641 acaagtcgaa gccgtgccgt gttgtgccgc gcccgaggtg ggcgccacag cacatatagc
     5701 cacgatgtag agcggggtgc gacggaggac ctcgccgggg gcgcgaggtc
//
LOCUS       DASVOR010001372         2771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_364812,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001372 DASVOR010000000
VERSION     DASVOR010001372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364812"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2768
                     /locus_tag="VF516_26120"
     CDS             <1..2768
                     /locus_tag="VF516_26120"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8111243.1"
                     /translation="EFFSRGRTNRSEPWAGDIFNGITGTAATVSAPAAPTLTLHGTVT
                     AGADHAPQPGASVSIAALGIVVFTDENGAYTMSVPPGSHVVRIEVAGYQPVDRTVTVA
                     AAPAALDVQLDETGAGEVITIIGSRTPRSRLETPVPVDVIGNDVIGESGYTEMNQMLN
                     AVAPSFNASHLSITDGTDHIDPADLRGLGPEHVLVLINGKRLHQSSLINVYNGGTVGV
                     DLNAIPTSAIARIEVLRDGAASQYGSDAIAGVINIVLKDTVDLVDLYSMTGVTAAGDG
                     GQLKLGANTGMKLGDHGFVNLTGEFFSRGRTNRSEPWAGDIFNGITGTAATDAELRRR
                     GLTRNDFTMDVGTSGALVGTGFLNAGYKLDSTFELYAQGGYTYRKGNASGFYRLPNAD
                     FENRSDLRIYPNGFLPQINPLLMSWTATGGVRARSGGSQGDLSLTYGGDSFHFFVDHS
                     LNASLGLASPTSFDAGTLGLTQTSLNFDGVQRIDQGALQALSLVAGAELRREDYRIRA
                     GQPESYELGPELNAEGEPKSPGSQVFPGFRPSDASNNSRLSEAVYAGVESKPTTGTNL
                     DVGARFEHYSDFGNTLTGKIAGRVAVLRAGDSEVALRGSASTGFRAPGLQQIYYSTIA
                     TQFLNDPATGAVVPSNILISPNRSDVTEKAFGVPNLKEETSVNFSGGVTARLKNLSIS
                     TDYYHVTLKDRVVLTSLFQTSDMVLGDTIKDILAQFPGVGAAQFFVNAVDTTTDGVDL
                     VADYSFHLPRGNLKVATALNYTRTVVDDVHVPQSMQEKFSSIQGGSQRVQDLFLGRYG
                     RNLLEDRLPRLKETLGLRWDYGIFTAGVRASYFGPSEYHSDDGPQLDESYGFKVIFDA
                     DVGYRVHGMTVIIGATNLFNTFPDQMKHEDNRYNNSFLYSPASVPAGTPFGTDGAFYY
                     VRMEYQH"
BASE COUNT          483 a         1024 c          878 g          386 t
ORIGIN      
        1 gcgagttctt ctcgcgcggg cggaccaacc gctccgagcc gtgggccggc gacatcttca
       61 acgggatcac cgggaccgcg gcgaccgtgt cggcgccggc cgcaccgacg ctgacactgc
      121 acggcacggt gaccgccggc gccgaccacg cgccgcagcc cggggccagc gtcagcatcg
      181 ccgcgctcgg catcgtggta ttcaccgacg agaacggcgc gtacacgatg tcggtgccgc
      241 ccggatcgca cgtggtccgg atcgaggtgg ccggctacca gccggtcgac cggaccgtga
      301 ccgtggcggc ggcccccgcc gcgctcgacg tccagctcga cgagacaggc gcgggtgagg
      361 tgatcacgat catcggctcg cgcacgccgc gcagccgcct cgagaccccg gtgccggtcg
      421 acgtgatcgg caacgacgtg atcggcgagt cgggctacac cgagatgaac cagatgctga
      481 acgcggtcgc gccgtcgttc aacgccagcc acctcagcat caccgacggc accgaccaca
      541 tcgaccccgc ggacctgcgc ggcctcggcc ccgagcacgt gctggtcctg atcaacggca
      601 agcgcctgca ccagagctcg ctgatcaacg tctacaacgg cggcacggtc ggcgtcgacc
      661 tcaacgcgat cccgaccagc gcgatcgccc ggatcgaggt cctgcgcgac ggcgccgcct
      721 cgcagtacgg ctcggatgcg atcgccggcg tgatcaacat cgtgctcaag gacacggtcg
      781 acctcgtcga cctgtactcg atgaccggcg tcaccgcggc gggcgacggc gggcagctca
      841 agctcggcgc caacaccggc atgaagctcg gcgatcatgg gttcgtcaac ctgaccggcg
      901 agttcttctc gcgcgggcgg accaaccgct ccgagccgtg ggccggcgac atcttcaacg
      961 ggatcaccgg gaccgcggcg accgacgccg agctccggcg ccgcggcctg acccgcaacg
     1021 acttcacgat ggacgtcggc acctcgggcg cgctggtcgg gaccggcttc ctcaacgccg
     1081 ggtacaagct cgacagcacg ttcgagctct atgcccaggg cggctacacc taccgcaagg
     1141 gcaacgcctc cgggttctac cggctgccga acgccgactt cgaaaatcgc agcgacctgc
     1201 ggatctaccc caacggcttt ttgccccaga tcaacccgct gctcatgtcg tggacggcga
     1261 ccggcggcgt gcgcgccagg tccgggggat cgcagggcga cctcagcctg acctacggcg
     1321 gcgacagctt ccacttcttc gtcgatcaca gcctgaacgc gtcgctcggc ctggcgagcc
     1381 cgaccagctt cgacgccggc acgctcggcc tgacccagac cagcctcaac ttcgacggcg
     1441 tgcagcgcat cgatcagggc gcactccagg cgctgtccct ggtcgcaggc gccgagctcc
     1501 gccgcgagga ctaccggatc cgggccggcc agccggagtc gtacgagctc gggccggagc
     1561 tcaacgcgga gggcgagccc aaatcaccgg gctcgcaggt gttccccggg ttccggccgt
     1621 cggatgcctc caacaacagc cggctcagcg aggcggtcta cgccggcgtc gagagcaagc
     1681 cgaccaccgg caccaacctc gacgtcggcg cccggttcga gcactacagc gacttcggca
     1741 acacgctgac cggcaagatc gccggccggg tcgcggtgct gcgcgccggt gacagcgagg
     1801 tcgcgctgcg cggctcggcg tcgaccggct tccgcgcccc gggcctgcag cagatctatt
     1861 acagcacgat cgccacgcag ttcctcaacg atcccgccac cggcgcggtc gtgccgagca
     1921 acatcctgat cagccccaac cgcagcgacg tcaccgagaa ggcgttcggg gtccccaacc
     1981 tcaaggagga gacctcggtc aacttcagcg gcggcgtcac cgcgcggctc aagaacctgt
     2041 cgatcagcac ggactactac cacgtcaccc tcaaggaccg cgtcgtgctc acgtcgctgt
     2101 tccagaccag cgacatggtc ctcggcgaca ccatcaagga catcctggcc cagtttcccg
     2161 gggtcggcgc ggcgcagttc ttcgtcaacg cggtcgacac gaccaccgac ggcgtcgacc
     2221 tcgtggccga ctacagcttc catctgccgc gcggcaacct caaggtggcc accgcgctca
     2281 actacacccg caccgtggtc gacgacgtcc acgtgccgca gagcatgcag gagaagttca
     2341 gcagcatcca gggtggctcg cagcgcgtgc aggacctgtt cctgggtcgc tacggcagga
     2401 acctgctcga ggaccggctc ccccgcctca aggagacgct cggcctgcgc tgggactacg
     2461 ggatcttcac cgcgggtgtg cgcgccagct acttcggccc gagcgagtac cacagcgacg
     2521 atggtccgca gctcgacgag tcgtacggct tcaaggtgat cttcgatgcc gacgtcggct
     2581 accgggtgca cggcatgacg gtcatcatcg gcgcgaccaa cctgttcaac accttccccg
     2641 atcagatgaa gcacgaggac aaccgctaca acaacagctt cctgtacagc ccggcgtcgg
     2701 tccccgccgg cacgccgttc ggcaccgacg gcgcgttcta ctacgtgcgc atggagtacc
     2761 agcactgacc g
//
LOCUS       DASVOR010001373         2901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_102471,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001373 DASVOR010000000
VERSION     DASVOR010001373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2901
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102471"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1335)
                     /locus_tag="VF516_26125"
     CDS             complement(<1..1335)
                     /locus_tag="VF516_26125"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111244.1"
                     /translation="MQSRLGRYVVLKHLASGGMADVLLGRTEGIEGFERHVVLKRIKP
                     EHAKDVRFIRMFLDEARVAANLHHQHIVQVHDIGETAGEYFIAMEYLHGEDVRTILST
                     ASKMRQHVPLGCAMAVVSAAAAGLHYAHERRGPDKRPLGIVHRDISPSNILVGYDGSI
                     KVVDFGIAKASARQETRSSSLKGKISYMSPEQCKGAEVDRRSDVYSLGVVLYELATTT
                     RLFKGESDYLVMDQIVNGRVTLPQVRRPELPNELSAIIMRAIAPDPERRYFTADELRV
                     ALDQFAAKASLTTSTSAIATYMRKMFGEKPEPWLELEGQDFDSFDEIGEPPVTESTLG
                     KSWTELPQGEEFRRSTSSIPRQSGPIAVIAGSATSETSRSQVSRLTSAPTPTDSKMGW
                     ESQQQAAAPRGFTPTKIAMICVPVIALLVIAIWRLAGTGSSTSPVAAATAPPP"
     gene            complement(1430..2653)
                     /locus_tag="VF516_26130"
     CDS             complement(1430..2653)
                     /locus_tag="VF516_26130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8111245.1"
                     /translation="MAVPSAGGRRRPGHVYAVVPVGYDCWMDLSLTEEHEALVQTARE
                     FTRKEITPRAAHHDETGEFPREILKKAWSTGLMNIEIPAEYGGLGGSCFDNCLVQEEV
                     SFGCSGFNTSMAANSLGATPLLVAGSEDQKRKYLSRLTAEFSFCAYCCSEPDAGSDVA
                     GMRTRVTRHGDDYVLNGQKRWITNGGVAGFYTVFATFDPALRHKGIACFVVDADTPGV
                     KAGRKENKMGQRASNTTDVIFEDCKIPKSQLVGTEDAGFKVAMKTFDRSRPWIAAMAA
                     GVIRRALEESRAYALERKTFGVPIAQHQAVQFMLAEMAMSYEATRLLTHKAAWQVDRG
                     DLSAITSSYAKAFGADAAMRSTTDAVQIFGGYGYTKEYPVEKLMRDAKLLQIYEGTSQ
                     IQRVVIARNVLGGKS"
     gene            complement(2590..>2901)
                     /locus_tag="VF516_26135"
     CDS             complement(2590..>2901)
                     /locus_tag="VF516_26135"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8111246.1"
                     /translation="VPAAPGPPRVVDAPPALLSHALVERVAAVRARELSACDGGEELR
                     GEVTVRFAVDAAGRVSNAQVATTLRKPRVAACILRSVQKWQFPPQGDAGAQGTYTLSF
                     Q"
BASE COUNT          428 a         1020 c          955 g          498 t
ORIGIN      
        1 gggcggaggc gcggtcgcgg ccgccaccgg cgaggtcgag ctgccggttc cggcgagccg
       61 ccagatcgcg atcaccagca gcgcgatcac cggcacgcag atcatcgcga tcttggtcgg
      121 cgtgaacccg cgcggtgcgg cggcctgctg ctggctctcc cagcccatct tggaatcggt
      181 cggcgtcggc gcgctggtca gccgcgacac ctgcgaccgc gaggtctcgg acgtggcgct
      241 gccggcgatc acggcgatcg ggccgctctg ccgcgggatc gagctcgtgc tgcggcggaa
      301 ctcctcgccc tgcggcagct cggtccagct cttgcccagc gtggactcgg tgaccggcgg
      361 ctccccgatc tcgtcgaacg agtcgaagtc ctggccctcg agctcgagcc acggctcggg
      421 cttctcgccg aacatcttgc gcatgtacgt cgcgatcgcc gacgtcgacg tggtcaggct
      481 ggccttggcg gcgaactgat cgagcgccac gcggagctcg tcggcggtga agtaccggcg
      541 ctcgggatcg ggcgcgatcg cgcgcatgat gatcgccgac agctcgttgg gcagctcggg
      601 ccggcggacc tgcggcagcg tgacccggcc gttgacgatc tggtccatga ccaggtagtc
      661 gctctcgccc ttgaacagcc gcgtcgtggt cgccagctcg tagagcacga cgccgagcga
      721 gtacacgtcg ctgcggcgat cgacctcggc gcccttgcac tgctccgggg acatgtacga
      781 gatcttgccc ttgaggctgc tcgaccgggt ctcctggcgc gccgacgcct tggcgatccc
      841 gaagtcgacg accttgatcg agccgtcgta gccgaccagg atgttcgacg gcgagatgtc
      901 gcgatgcacg atgccgagcg gccgcttgtc gggcccgcgc cgctcgtgcg cgtagtgcag
      961 ccccgccgcc gcggccgaca cgaccgccat cgcgcagccc agcggcacgt gctggcgcat
     1021 cttcgacgcc gtcgacagga tcgtccgcac gtcctcgccg tgcaggtact ccatcgcgat
     1081 gaagtactcg ccggcggtct cgccgatgtc gtggacctgc acgatgtgct ggtggtgcag
     1141 gttggcggcg acccgcgcct cgtcgaggaa catccggatg aaccgcacgt ccttggcgtg
     1201 ctccggcttg atccgcttga gcacgacgtg gcgctcgaag ccttcaatgc cctcggtccg
     1261 tcccagcaga acgtctgcca ttccgcctga cgccaggtgc ttgagcacca cgtatcgacc
     1321 gagccgcgac tgcattacga ggcacagcgt atgaaattgt cagggtttcg ccaatcacca
     1381 ttcgcggccg cggttcagcc tacatgtcgc ccccggccgg gcgcccgggt cacgatttgc
     1441 caccgagcac gttccgggca atgaccactc gctggatctg cgaggtgcct tcataaatct
     1501 gcaacagctt cgcgtcgcgc atcagtttct cgacggggta ctccttggtg tatccatacc
     1561 ccccgaaaat ctgaaccgcg tccgtcgtgc tccgcatcgc agcatcggca ccgaaagcct
     1621 tggcatagct cgatgtgatc gccgagagat ctccccggtc cacctgccag gcggccttgt
     1681 gcgtcaagag ccgggtcgcc tcgtaggaca tggccatctc ggcgagcatg aactgcacgg
     1741 cctggtgctg tgcgatcggc acgccgaagg tcttgcgctc cagggcatag gcccggctct
     1801 cctccagggc gcgccggatc accccggcgg ccatcgccgc gatccagggc cgcgagcggt
     1861 cgaacgtctt catcgccacc ttgaaccccg cgtcctcggt gccgaccagc tggctcttcg
     1921 ggatcttgca gtcctcgaag atcacgtcgg tggtgttcga cgcccgctgc cccatcttgt
     1981 tctccttgcg cccggccttg acgcccgggg tgtcggcatc gacgacgaag cacgcgatcc
     2041 ccttgtggcg gagggcgggg tcgaaggtgg caaatacggt gtagaaccct gcgacccccc
     2101 cgttggtgat ccagcgcttt tgaccgttga gcacgtagtc gtcgccgtgg cgcgtgaccc
     2161 gggttcgcat ccccgcgaca tccgaaccgg cgtcgggctc gctgcagcag taggcgcaga
     2221 acgagaactc ggccgtgagc ctcgacaggt acttccgctt ctggtcttcg ctgccggcca
     2281 ccagcagcgg ggtggccccg agcgagttgg ccgccatcga agtattgaac cccgagcagc
     2341 cgaacgagac ctcctcctgc accaggcagt tatcgaagca gctgccgccc agcccgccgt
     2401 actcggccgg gatctcgatg ttcatcaggc cggtgctcca cgcttttttc aggatctcgc
     2461 gcggaaactc gccggtctcg tcgtggtgcg cggcccgggg ggtgatctcc ttgcgggtga
     2521 actcgcgggc ggtctggacg agcgcttcgt gctcttcggt gaggcttagg tccatccagc
     2581 agtcatagcc tactggaacg acagcgtata cgtgccctgg gcgccggcgt ccccctgcgg
     2641 agggaactgc cacttttgca ccgagcgcag aatgcacgcg gcgacccggg gcttgcgcag
     2701 cgtcgtggcg acctgcgcgt tgctcaccct gccggcggca tcgaccgcga accgcaccgt
     2761 gacctcgccc cgcagctcct cgccgccgtc gcacgccgac agctcgcgcg cgcgcaccgc
     2821 ggcgacccgc tcgaccagcg cgtgggacag cagcgcgggc ggagcatcga cgacccgggg
     2881 cgggcccgga gccgcaggga c
//
LOCUS       DASVOR010001374         2643 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393446,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001374 DASVOR010000000
VERSION     DASVOR010001374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2643
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393446"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /locus_tag="VF516_26140"
     CDS             complement(<1..768)
                     /locus_tag="VF516_26140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111247.1"
                     /translation="MSSDERLDERFQLESRIGSGGMGEVFRARDPVTGEVVAIKILPG
                     SQGPLAERFTREIEVLAKLDHPGIVRYISHGMTPASEPFLVMEWLDGESLKVRLERGA
                     LTVAEAITLATRVAGALGAVHARGIVHRDLNPSNLFLPGGHVEQVKVLDFGIAQWHGR
                     TQLTRTGMILGTPGYMAPEQARIGGAIDARADVFALGCVLTHCLTGVAPFAGTDTAAV
                     LAKILFGAPPRLRELWPDAPADLDALLTRMLAQEPSLR"
     gene            complement(872..1378)
                     /locus_tag="VF516_26145"
     CDS             complement(872..1378)
                     /locus_tag="VF516_26145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111248.1"
                     /translation="MKPPRRRALRHEDAVLALVLLAIAIPAAIALPMVWLGELDLKSR
                     LTLTLAVAAGSGGLALAVRSRVMRPLQTLANLTASLRERDYAVRGRHVRGDDSLGLAI
                     AELAQLAAPDRRGARRLAGGPQPPDRPRRRGGDQPASDRARALGVIRSRPRRGMSHRV
                     APWRVHRP"
     gene            complement(1455..2051)
                     /locus_tag="VF516_26150"
     CDS             complement(1455..2051)
                     /locus_tag="VF516_26150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111249.1"
                     /translation="MTNLFLISNTSTILPALAVALSAGCATEPEPDAAAADGVAEVVQ
                     NVTTLSGCEYQVDLRPEIQAQTVAISTSSYAARGCDSFQLRVITGGHDWRAYVTWHGV
                     NLDADANACNNSLVFAALSRHDASGLTYLGTKIADGAWGTVIGTVPVAGGSLPIYGCN
                     SPDVNWGLGDHLSYEMSVFGFLSGTGPQGVYVQFVPLD"
     gene            complement(2194..2406)
                     /locus_tag="VF516_26155"
     CDS             complement(2194..2406)
                     /locus_tag="VF516_26155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111250.1"
                     /translation="MSAGADSLQYLRGWMAALDEMRRPDWREVYDQTSRDIAAGRGRD
                     LETVAQELGLESRSPHGRPRHRPKRR"
     gene            complement(2403..>2643)
                     /locus_tag="VF516_26160"
     CDS             complement(2403..>2643)
                     /locus_tag="VF516_26160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111251.1"
                     /translation="LMIPSVVRVLGAAGSPLVPWGWGVNGATSVIGTVIATVIAIYGG
                     FTATLLVGAAGYLAAGLLGWRVAGAHPPAAEESTR"
BASE COUNT          386 a          951 c          934 g          372 t
ORIGIN      
        1 ccgcagcgac ggctcctgcg ccagcatccg ggtgagcagc gcgtcgaggt ccgcgggggc
       61 gtcgggccac agctcgcgga gccgcggcgg cgcgccgaac aggatcttgg ccagcaccgc
      121 ggcggtgtcg gtgcccgcga acggcgcgac cccggtcagg cagtgggtca gcacgcagcc
      181 gagcgcgaac acgtcggccc gcgcatcgat cgccccgccg atgcgcgcct gctccggtgc
      241 catgtacccc ggcgtcccca ggatcatccc ggtccgggtg agctgggtcc gcccgtgcca
      301 ctgcgcgatc ccgaagtcga gcaccttgac ctgctcgacg tgaccgccgg gcaggaacag
      361 gttggacggg ttgagatcgc ggtggacgat cccgcgcgcg tgcaccgcgc cgagcgcgcc
      421 ggcgacccgg gtcgcgagcg tgatcgcctc ggccacggtg agcgcgccgc gctcgaggcg
      481 gaccttgagg gattccccgt cgagccactc catcacgagg aacggctcgc tggcgggcgt
      541 catgccgtgc gagatgtaac ggacgatgcc ggggtgatcg agcttggcga gcacctcgat
      601 ctcgcgcgtg aaccgctcgg cgagcgggcc ttgcgatccg ggcaggatct tgatcgccac
      661 cacctcgccg gtgaccggat cgcgggcgcg aaacacctcg cccatgccgc cgctcccgat
      721 ccgcgactcg agctgaaacc gctcgtcgag ccgttcgtcg gagctcatgg cgcccagcgc
      781 ccctctacta cctggttgtg gtccagggtc cacacagccg ggatgagtcc gacaagatac
      841 cactcgtcca ttgccactgg aatggacatc ttcacgggcg atggactcgc catggtgcga
      901 cgcggtggga catgccgcgg cgaggacggg agcggattac cccgagcgct cgcgcacggt
      961 cgctggcagg ctgatcaccg cctcggcgcc gcggccgatc cggcggttgc ggaccaccag
     1021 cgagccgccg tgcgcctcgg cgatctggcg cggcgagctg cgccagctcg gcgatcgcga
     1081 gcccgagcga gtcgtcgccg cgcacatggc ggccgcgcac cgcgtagtcg cgctcgcgca
     1141 gcgacgcggt caggttggcc agcgtctgca gcggccgcat cacccgcgac cgcaccgcca
     1201 gcgccagccc gccgctgccg gctgcgaccg ccagcgtcag cgtcagccgc gacttcaggt
     1261 cgagctcgcc gagccacacc atcggcagcg cgatcgccgc cgggatcgcg atcgcgagca
     1321 gcaccagcgc gaggacggca tcctcgtgac gcagggcgcg gcgccgcggc ggcttcatcc
     1381 ggccatggta cgcgcaccgg ccacgaggtc tccatcgcgg ccgccatccg ctcggcgatc
     1441 ggcggcccgg gtcttcagtc cagcggcacg aactggacgt agacgccctg cggcccggtt
     1501 ccggacagga agccgaacac gctcatctcg tagctgaggt ggtcgccgag gccccagttc
     1561 acatcgggcg agttgcagcc gtagatcggc agtgatccac cggcgaccgg cacggtgccg
     1621 atgacggtgc cccaggctcc gtcggcgatc ttcgtgccca ggtaggtcag gccggacgcg
     1681 tcgtggcggc tgagcgccgc gaacaccagc gagttgttgc acgcgttcgc gtcggcatcg
     1741 aggttgacgc cgtgccaggt cacgtaggcg cgccagtcat ggccgccggt gatgacccgc
     1801 aactggaagc tatcgcagcc cctcgcggcg tagctcgacg tcgagatcgc cacggtctgt
     1861 gcctggatct cgggccgcag gtcgacttga tactcgcagc cggacagcgt cgtgacgttc
     1921 tgcacgacct cggcgacgcc gtcggctgca gctgcgtcgg gctctggctc ggtggcgcag
     1981 ccggcgctca gcgcgacggc cagcgcaggc aggatggtcg atgtgttcga tatgaggaat
     2041 agattcgtca tctgtcactc ccagcaatac gtctccgccg ccgtgcccca cgcacggcga
     2101 tcgcctggag ctacgggtcg catggctggc agttacgacg aatcgagcga ggcgcgtcga
     2161 actaccggct tcgcttcgcg gcaagcgctg ccctcagcgc ctcttcgggc ggtggcgggg
     2221 gcggccgtgc ggagaccggc tctccagacc cagttcctgg gcgaccgttt cgaggtcgcg
     2281 ccctcgaccg gcggcgatgt cccggctggt ctgatcgtag acctctcgcc agtcgggccg
     2341 gcgcatctcg tcgagcgcgg ccatccatcc acggaggtac tgcaggctgt ctgctccagc
     2401 gctcatctcg tcgactcctc cgcggccggg ggatgcgcgc cggcgacccg ccagccgagc
     2461 aggccggcgg cgaggtagcc cgcggcgccg accagcagcg tggcggtgaa cccgccgtag
     2521 atcgcgatca cggtcgcgat cacggtgccg atcacggagg tcgcgccgtt gacgccccag
     2581 ccccacggga cgagcgggct gcccgcggcg ccgagcacgc ggacgaccga cgggatcatc
     2641 agg
//
LOCUS       DASVOR010001375         6315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_335341,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001375 DASVOR010000000
VERSION     DASVOR010001375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6315
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335341"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1079)
                     /locus_tag="VF516_26165"
     CDS             complement(150..1079)
                     /locus_tag="VF516_26165"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8111252.1"
                     /translation="MRLHDRAYRASHRPSRALARTSAANRAVGSRGRNVIAAIGVDRY
                     QRWPRLANAARDAMGALQLFRRLGFESVTEPLLDDRATGAALQALVTDDLAGLGPDDS
                     LVLFYAGHGGTRQHHLGDRVVRTGYLIPVDASDAPGKVSTWIELEGWLRAVALLPARH
                     ILVILDACHSGIALDPAIRWRDVGWAGDAPLPTLNARRSRRIITSALDDQVACDGGPV
                     RGHSLFTGCLIEALTGGLRARHGGVTTGSELGAYLQRRVESYPGSCQTPDFGAFAFDD
                     RGEMRIPVAAPRRARSSVDRCRCGAVARARCTV"
     gene            1577..2215
                     /locus_tag="VF516_26170"
     CDS             1577..2215
                     /locus_tag="VF516_26170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111253.1"
                     /translation="MFRSSTAQPGVRSPSRNPVPSLNPDDDGGPRERTSDPRSFLTRQ
                     RLHLYLKTSRHAELRRELARAELGGEISEFYALSFQAVLAMAEGSELAADYLEMAEAV
                     ASSPYERAIIAEDRAAYDLLHDNPSAAAERCLATLDHVHQTEGLWVSLLIALYRLGKV
                     DTIDATLRRLTRLDGECTMRIVGLLSTDPDLGGVRARPSCKQLLGQHAAGGR"
     gene            2380..3063
                     /locus_tag="VF516_26175"
     CDS             2380..3063
                     /locus_tag="VF516_26175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111254.1"
                     /translation="MLEYTISKPEFSPPPCNRHLDELFADPGFRKTVDLVAREVMRLW
                     GVPHAAAHGFVVSAIGEPDTLACIYNAWSVATSTGENFGLVKVIIRRRVIDLLRKDAR
                     QANHCSLPTTVDAIDTDRTPAFHELVQRDPRLQLELQQIIHMVRGALDCFSTQGEIQD
                     RQARLLRRYALDEMDYTSLSAELVCSESALRVRVHKAMLALRKHIRECHTELEDLLDR
                     DRGMAAARG"
     gene            3279..4793
                     /locus_tag="VF516_26180"
     CDS             3279..4793
                     /locus_tag="VF516_26180"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8111255.1"
                     /translation="MPLCGAAACLILACSANGTGAGGAAPPPDVGIHKVKHVIVVMQE
                     NHSFDSYFGALVYAPGSPYHAPAAAGTSCDDSDHCCVDGLSCTADAGGGLRCSNANLD
                     ENGGIVAAFHNPSRCVPDPDHSWTGVHRQINYDQPNAARSEPRMDGFVRVSHSIQPMG
                     FYTQDDLPFYYDLAQRFAIDDRFFAPVLGPTFPNRAYLMAATSFGHLVTSDQIPPLAG
                     FKPIHGTIWDLLDRNHVSWVDYYQDVPQGASFRVLGSTLFSSHFAPLSLFLAHAAGRG
                     ELPSVAFVDPNFGAFREASRNDEEAPTDIQRGQAFVSRVINAVRNGPRWADSVIFVVY
                     DEHGGLYDHVPPPPAIPPDTIEPGQCADLSDPPASLLPGGGAECSSNHKSSSDVTVAQ
                     ATALCPALAARPTGLYPADCAHFDQYGVRVPFVAVSPFSRPQYVSHTIADHTSILAFI
                     ETAFLPPVGGQRQYLTARDQSADNLLDLFDFDGSPSLHATVGTAMPPADDCTPR"
     gene            4952..>6315
                     /locus_tag="VF516_26185"
     CDS             4952..>6315
                     /locus_tag="VF516_26185"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8111256.1"
                     /translation="MSVWPRVLYPSLPLAGGMACLLTACATGGPPDGAADVAAAAVLD
                     AARADGGIHKVQHVIVVMQENHSFDNYFGALAYAPGSPYHAPAAGRAGCAGNDHRCLD
                     GLRCAIDAGGALQCSNTNLDDDGSTVAAFHDPNRCPAPDIDHGWASTHREVNFAQPNA
                     TRQQPLLDGFVRVNDETEQQDGAESPTDDETIGFYTQDDLPFYYDLAQHFAISDRFFS
                     PMLGPTFPSRAYFAAATSFGHVSTADQIPPLEGYRPVGGTIWDLLDRNGVSWADYYQD
                     LPQAASFRPLGSTVIDPHFQLLPVFLAQAAGVGDLPSVSFVDPNFGALGTATENDEHP
                     PTDIQRGQAFVSQVVSAVRNGPHWTDSVIFIVYDENGGFYDHVPPPPAVPPDAAQPGQ
                     CEDLSNPPASLQPGGGAECSSNLQGDPDTSLAEAIALCPALAADPTGPYPAGCARFDQ
                     LGVRV"
BASE COUNT         1021 a         2353 c         2049 g          892 t
ORIGIN      
        1 gatcgcggca tagacctcac ccatcgcgcc ggtgccgagc gtcccggtca cccggtagcg
       61 accgaagctg tgcggatctg ccacgccgca aggataacgc ggatcgcccc gcccacgcac
      121 cgcgcgaccg aggtcggtgc cccggcggct cacaccgtgc accgggcacg cgcgactgca
      181 ccgcagcggc agcggtccac cgacgatcgc gcccggcgcg gcgccgcgac cgggatccgc
      241 atctcgccgc ggtcatcgaa cgcaaacgcc ccgaagtccg gcgtctggca agagcccgga
      301 tagctctcca cccgccgctg caggtacgcg ccgagctccg accccgtggt cacgccgccg
      361 tggcgcgccc gcaggccccc ggtgagcgcc tcgatcaggc agccggtgaa cagcgagtgt
      421 ccgcggaccg gaccgccatc gcacgcgacc tggtcgtcca gcgccgacgt gatgatccgc
      481 cggctgcgcc gcgcgttcag cgtgggcagc ggcgcatctc ccgcccagcc cacgtcccgc
      541 caccggatcg ccggatcgag cgcgatgccg ctgtggcacg cgtcgaggat cacgaggatg
      601 tgccgggccg gcagcaacgc gaccgcgcgc aaccagccct ccagctcgat ccacgtcgag
      661 accttgcccg gcgcgtcgga ggcgtcgacc gggatcaggt atccggtcct gaccacgcga
      721 tccccgaggt ggtgctgccg cgtgccgccg tggccggcgt agaacagcac gaggctgtcg
      781 tctggcccga gccccgcgag gtcatcggtc accagcgcct gcagcgccgc gccagtcgct
      841 cggtcgtcga gcagcggctc ggtgaccgac tcgaagccaa gccgccgaaa cagctgcagc
      901 gcgcccatcg catcacgtgc cgcattggcc agtcgtggcc agcgctgata gcgatcgaca
      961 ccgatcgcag cgatcacgtt gcggccgcgc gagccaaccg ctcggttcgc ggcggaagta
     1021 cgcgcgagcg cacgcgacgg gcgatggctc gcccggtatg cccggtcgtg gagacgcatg
     1081 ggcgacggcg ggaatcgaag cggtgaatcg agcgccggac ggcgaacgac cggcccggct
     1141 tcgccgtgtc gtagccggcg cgaaaaacgt tatcccggcc ccgagtccac aaacgtggac
     1201 ggatccgggc ccgcgattcc acagaactgg acatcccggc gcgacaaacc gcgaatggtc
     1261 tcgcagacca ccggaatttc cagcaccctg ccccgattcg aatcattccg gatcacggct
     1321 aacgccgccg cgagcacctg cgatacagca acaagcgccg gtgccgcgcc gcggagtgac
     1381 accggacagc aggatgcgaa gttcgggctc cgttccgtgg tggaaagccg cccgactccg
     1441 ccagtcatca ttgcaagaaa gcgaacaaac gatgcaggca aattcatcca ctgcgaatgg
     1501 cagtgaagat ctgaccccgc tccgcgccgc ccacctcgcg gctcgactca cggagcggct
     1561 tcggcgcagc gctcccatgt tcaggagctc caccgcgcaa ccgggcgttc gcagcccgag
     1621 ccgcaacccg gtgccgtcgc tgaatccgga cgacgatggc ggtcctcgcg agcgaacgag
     1681 cgatccacgc agcttcctga ctcgccagcg gctccacctc tatctgaaga ccagccggca
     1741 cgccgagctc cgccgcgagc tcgcgcgcgc cgagctcggg ggagagatct cggagttcta
     1801 cgcgctgagc ttccaggcgg tgctggcgat ggcggagggc tcggagctcg ccgcggacta
     1861 cctcgagatg gccgaggcgg tcgcgtcgtc gccgtacgag cgggcgatca tcgcggagga
     1921 ccgggcggcg tacgacctgc tgcacgacaa cccgtcggcc gccgccgagc gctgcctggc
     1981 caccctcgat cacgtccatc agacggaggg actgtgggtc agcctcctga tcgcgctcta
     2041 ccgcctgggc aaggtcgaca ccatcgatgc cacgctgcgg cgtctgacgc ggctcgatgg
     2101 cgagtgcacc atgcgcatcg tggggctgct gtcgaccgac ccggacctgg gcggcgttcg
     2161 cgcgcggccg tcatgcaagc agctgctcgg acaacacgct gcgggagggc gctaacggac
     2221 ccagccatcg cgacgactcc gtacattgca atcctgcttg acgacccctc gaacgcaaac
     2281 ccgacgaatg gagaagcagc catcaacaat ctcgatcatc gacaacgcgg tgtcctgaat
     2341 ggcaactcgg ccataccggc ggaaggcccg attaccacca tgctggaata taccatatca
     2401 aagccagagt tctctccacc accttgcaac cgccacctcg acgagctgtt cgcggatccc
     2461 gggttccgga agaccgtgga tctggtcgcc cgggaggtca tgcgcctgtg gggcgtcccc
     2521 cacgccgcgg cgcatggctt cgtggtctcg gcgatcggcg agcccgatac gctggcgtgc
     2581 atctacaatg catggtcggt ggcgaccagt acgggcgaga acttcggcct ggtcaaggtg
     2641 atcatccggc gccgcgtgat cgacctgctc cgcaaggatg cccggcaggc caaccactgc
     2701 tcgctgccga ccacggtcga cgcgatcgac accgatcgga cgccggcgtt ccacgagctc
     2761 gtccagcgcg acccgcggct ccagctcgag ctccagcaga tcatccacat ggtccgcggc
     2821 gcgctcgact gcttctccac gcagggcgag atccaggacc gacaggcgcg gctgctccgg
     2881 cgctatgcgc tcgacgagat ggactacacc tcgctcagcg cggagctggt gtgctccgag
     2941 agcgcgctgc gggtccgggt tcacaaggcg atgctcgcgc tgcgcaagca catccgcgag
     3001 tgccacaccg agctggagga cctgctggac cgcgatcgcg ggatggccgc agcccgcgga
     3061 tagcgtgtgt cggtcggcca cggcgggtgt tccgtcgccg cccaccgagc gctccagcac
     3121 gggcgcccac cgcgcacgtg tccgcggagc acgggggtgg ggtcgcgatt tctcgcagtc
     3181 cgggatagac agactccctc gcagccttca caccttggaa aaccaggagt gcagggatgc
     3241 gtgtgttagg tgtagggtcg cgagccccgt gtccgagctt gccgctgtgc ggcgcggcgg
     3301 cgtgcctgat cctcgcatgc tccgccaacg ggacgggggc gggaggcgcg gcgccaccgc
     3361 ccgacgtcgg catccacaag gtcaagcatg tcatcgtcgt gatgcaggag aaccattcgt
     3421 tcgacagcta cttcggcgcg ctggtctatg cgccgggcag cccgtaccac gcgccggcgg
     3481 ccgccggcac cagctgcgac gacagcgacc actgctgcgt cgacggcctg agctgcacgg
     3541 ccgacgccgg cggcggcctg cgctgcagca acgccaacct cgacgaaaac ggcggcatcg
     3601 tggcggcgtt ccacaacccg agccgctgcg tacccgaccc cgatcacagc tggaccggcg
     3661 tgcaccgcca gatcaactat gaccagccca acgccgcgcg gagcgagccg cggatggacg
     3721 gcttcgtccg ggtcagccac tcgatccagc ccatgggctt ctacacccag gacgatctgc
     3781 cgttctatta cgacctcgcc cagcggttcg cgatcgacga ccggttcttc gccccggtgc
     3841 tgggcccgac gttccccaac cgcgcctatc tcatggccgc gacgtcgttc ggtcacctcg
     3901 tgacgtccga ccagatcccg ccgctcgcgg gcttcaagcc gatccacggc acgatctggg
     3961 acctgctgga tcgcaaccac gtgtcgtggg tggactacta ccaggacgtg ccgcagggtg
     4021 cgagcttccg cgtgctcggc tcgacgctgt tcagctccca cttcgcgccg ctgtcgctgt
     4081 tcctggccca cgcggcgggc cgcggcgagc tgccgtcggt ggcgttcgtc gatccgaact
     4141 tcggcgcgtt ccgcgaggcg agccggaacg acgaggaggc gccgaccgat atccagcgcg
     4201 gccaggcctt cgtgtcgcgc gtgatcaacg ccgtgcgcaa cgggccgcgc tgggccgact
     4261 ccgtgatctt cgtggtctac gacgagcacg gcgggctcta cgatcacgtg ccgccgcccc
     4321 ccgcgatccc gccggatacg atcgagcccg gccagtgcgc ggatctgtcg gatccaccgg
     4381 ccagcctcct gcccggcggc ggcgccgagt gcagcagcaa ccacaagagc agctcggacg
     4441 tcaccgtggc ccaggcgacc gcgctgtgcc ccgcgctggc ggcccgcccc accgggctgt
     4501 accccgcgga ctgtgctcac ttcgatcagt acggcgtccg ggtcccgttc gtcgcggtgt
     4561 cgccgttctc caggccgcag tacgtctcgc acacgatcgc cgaccacacg tcgatcctcg
     4621 cgttcatcga gaccgcgttc ctgccgccgg tgggcggtca gcgccagtac ctgaccgcac
     4681 gcgaccagag cgccgacaac ctgctcgacc tgttcgactt cgatggctcg ccgtcgctcc
     4741 acgcgaccgt cggcaccgcg atgcctcccg cggacgactg cacgccgcgg tgatcgcgag
     4801 cgcgagcgcc ccaccgtgcg cgccggcgtc cgctcggcgt gccgtcgcgc cacaccgtgc
     4861 ctcgatgagt cgagcgtgat gtcgccatcg cggataaaac acattcagtc gctggccatc
     4921 acacccaggg aacctctgca ggagtcccaa catgagtgtt tggccgcgag tcctgtatcc
     4981 gagcctgccg ctggccggcg gcatggcgtg tctgctcacc gcatgcgcca ccggcggtcc
     5041 tccggacggc gccgcagacg tcgcggcagc ggcggtgctc gacgcggcgc gggccgacgg
     5101 cggcatccac aaggtccagc acgtcatcgt ggtcatgcag gagaaccact cgttcgacaa
     5161 ctacttcggc gcgctggcct acgcgcccgg cagcccgtac cacgcaccgg ccgccggccg
     5221 cgccggctgc gccggcaacg atcaccgctg cctcgacggc ctgcgctgcg cgatcgacgc
     5281 cggcggcgcg ctgcagtgca gcaacaccaa cctcgacgac gacggcagca cggtggccgc
     5341 attccacgac cccaaccgct gccccgcacc cgacatcgat cacggctggg ccagcacgca
     5401 ccgtgaggtc aacttcgcgc agcccaacgc gacgcgccag cagccgctgc tcgacggctt
     5461 cgtccgggtc aacgacgaga ccgagcagca agacggcgcc gagtcgccga ccgacgacga
     5521 gaccatcggc ttctataccc aggacgacct gccgttctac tacgacctgg cgcagcactt
     5581 cgcgatcagc gatcggttct tctcaccgat gctgggcccg accttcccca gccgggccta
     5641 cttcgcggcg gcgacgtcgt tcggccacgt gtcgaccgcc gaccagatcc cgccgctgga
     5701 gggctaccgg ccggtcggcg ggacaatctg ggacctgctg gatcgcaacg gcgtgtcgtg
     5761 ggccgactac taccaggacc tgccccaggc ggcgagcttc cgcccgctcg gctcgacggt
     5821 gatcgatccg cacttccagc tcctgccggt gttcctcgcc caggcggccg gcgtcggtga
     5881 cctgccctcg gtatcgttcg tcgatccgaa cttcggcgcg ctcggcaccg ccaccgagaa
     5941 cgacgagcat ccgccgacgg acatccagcg cggccaggcg ttcgtgtccc aggtggtcag
     6001 cgccgtgcgc aacgggccgc actggaccga ctcggtgatc ttcatcgtct acgacgagaa
     6061 cggcgggttc tacgaccacg taccgccgcc gcccgccgtc ccgcccgacg cggcccagcc
     6121 cggtcagtgc gaggacctgt cgaacccacc cgccagcctc cagcccggtg gcggcgcaga
     6181 gtgcagcagc aacctccagg gcgatcccga caccagcctg gccgaggcga tcgcgctgtg
     6241 ccccgcgctg gcggccgacc cgaccgggcc gtaccccgcc gggtgcgccc ggttcgatca
     6301 gctcggcgtc cgtgt
//
LOCUS       DASVOR010001376         4422 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_422561,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001376 DASVOR010000000
VERSION     DASVOR010001376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422561"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1106)
                     /locus_tag="VF516_26190"
     CDS             complement(54..1106)
                     /locus_tag="VF516_26190"
                     /inference="COORDINATES: protein motif:HMM:NF014601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8111257.1"
                     /translation="MTADADAGPILIWGAGAVGGTIGACLARAGHPVVVVDVVAEHVA
                     AIAERGLALEGPLGGFTQRLAAFTPDQLDAAAQQGGRYRRSLLCVKAQHTAAAAAALR
                     PFLSDDGYVVSAQNGLNERVIAEIVGPARTIGAFVNFGADYLGPGRVVYGGRGAVVVG
                     EIDGRRTPRLAALHRTLCDFEPAAVATDNIWGYLWGKLAYGALLFATALTHDSIADAL
                     ALPQYFAAYRALGAEVLGAAAAERVRPEGFNGFDPAAFEAGADPAATRRSIDEMVAHN
                     RRSAKSHSGIWRDLAVRKRRTEVDAQIAIVAEIAAAHRRTTPVLDRLVRLVHEIEDGQ
                     RPQGRANLDALAEAAR"
     gene            complement(1103..1804)
                     /locus_tag="VF516_26195"
     CDS             complement(1103..1804)
                     /locus_tag="VF516_26195"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEX8111258.1"
                     /translation="MKITEMNWRQVEAYLRDDDRAVVPIGSTEQHAGLSLGTDLVLAE
                     RVSVEAAEPLGVPVFPTLNYGYTPYFTAYPGTICLRIETLIALVRDVLDSLRRSGFRR
                     ILIVNGHGGNAPAGALLSEWMADHPGTCVKFHNWYNAPKVWAKVQDIDPVASHASWME
                     NFPWTRVSGVRYPDESKPMVDAERLRLLDPKRARELLGDGNFGGRYQRSDDDMWALWQ
                     VAVAETREMIEGPWA"
     gene            2052..3302
                     /locus_tag="VF516_26200"
     CDS             2052..3302
                     /locus_tag="VF516_26200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111259.1"
                     /translation="MYPSRVREGALLAALAAGLYVEASCGPKPPGTGGQPAVTAPSPC
                     ALPTAMAGSNEQTAWQLFVAASCPVGGKLAWETWTEQTCFNKPDSPGCGPRAGAAVDA
                     ARHLHGSPLGEGRRRARLKMASATPGPCSDMTTTANAPAELRPFVPKNLTANAKFCEE
                     VFVNEPEAAFVRAPSRGATLTNLTAQVSYAKFGAISFPTPSVEIKADWLPATSLNNAF
                     NCTTNPPDGVYVETISGTCYALVGIHVSSKLLPNWLWATFEPQNTVTNPNRCNPDLYS
                     ACNDPWGSSPPVSTGQATQPTPALAALMSQAKLAPQLLNYRLVAAQSLYVDANNNPIQ
                     LGNSFVEFNAEVPPHQASCITCHSYAMVSTTANENPNFGNFPGTPPIGMPGTAPLPAQ
                     GGGTWVTQDFSWMLGIMPQTPHHE"
     gene            complement(3313..>4422)
                     /locus_tag="VF516_26205"
     CDS             complement(3313..>4422)
                     /locus_tag="VF516_26205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8111260.1"
                     /translation="VDVVGLLLDRRVAIPVLVVRDGIIASSNEAARELLDGDVDQFIE
                     ALFTADSRSKLTGALAAAPASCEVQAQRGGRDPQPVRLVALPLGPDTRLVLVAHSGAE
                     YSEAMAQQLLQANNHLANLTRELSRQSAEIQAARTRFESLADLREHFVSMLAHEVRGG
                     LQGIALSLEVMERADASRSTELWNQARGRIHRSAAQVVELIEKVLEAARTESGRVILD
                     ARPVSIRAIARDALEIYTPIADRAGVGLELVDRLGDAVVAADRVRFGQVVGNLIENAI
                     RHSPSAGTVTVELSATAQVVDLAVRDQGPGIPVELRERLFERFIQGTGKSGSLGLGLY
                     VAHELVMLHGGRIRIEDVAPHGAALIVELPRSGES"
BASE COUNT          669 a         1654 c         1479 g          620 t
ORIGIN      
        1 gaagccgtgc gcgccgccgg tgacgatcac ggtctggccg gggaacgcga ggttcatcgc
       61 gcggcctcgg cgagcgcgtc gaggttggcg cggccctgcg gccgctggcc gtcctcgatc
      121 tcgtggacga ggcggaccag ccgatcgagc accggcgtgg tcctgcgatg cgccgccgcg
      181 atctcggcga cgatcgcgat ctgcgcgtcg acctcggtcc tgcgcttgcg caccgcgagg
      241 tcgcgccaga tcccgctgtg cgacttcgcc gaccgccggt tgtgcgcgac catctcgtcg
      301 atcgagcgcc gggtcgccgc cgggtccgcg ccggcctcga acgccgcggg gtcgaagccg
      361 ttgaacccct cggggcggac ccgctcggcc gccgccgcgc ccaggacctc ggcgccgagc
      421 gcgcggtagg ccgcgaagta ctgcggcagc gccagcgcat cggcgatcga gtcgtgggtc
      481 agcgcggtcg cgaacagcag cgcgccatag gccagcttgc cccacagata gccccagatg
      541 ttgtcggtcg cgaccgccgc cggctcgaag tcgcacagcg tccggtgcag cgccgccagc
      601 cgcggcgtcc tgcgcccgtc gatctcgccg acgaccaccg cgccgcgccc gccgtacacg
      661 acgcggccgg ggccgaggta gtcggcgccg aagttgacga acgcaccgat cgtgcgcgcc
      721 gggcccacga tctcggcgat cacgcgctcg ttgagcccgt tctgcgccga gacgacgtac
      781 ccgtcgtccg acaggaacgg ccgcagcgcc gccgcggccg ccgccgtgtg ctgcgccttg
      841 acgcacagga ggctccgccg gtagcgcccc ccctgctggg cggcagcgtc gagctggtcg
      901 ggcgtgaacg ccgccagccg ctgcgtgaag cccccgagcg ggccctcgag ggcgagcccg
      961 cgctcggcga tcgcggcgac gtgctcggcc acgacatcga ccacgacgac cgggtggccg
     1021 gcgcgcgcga ggcaagcgcc gatcgtcccg ccgaccgccc cggcgcccca gatcaggatc
     1081 ggcccggcgt ccgcgtccgc ggtcatgccc acgggccctc gatcatctcg cgcgtctcgg
     1141 cgaccgcgac ctgccacagc gcccacatgt cgtcgtccga gcgctggtag cgtccgccga
     1201 agttgccgtc gccgagcagc tcgcgcgccc gcttcgggtc gagcagccgc agccgctccg
     1261 catcgaccat cggcttcgat tcgtccggat accggacacc cgacacccgc gtccacggga
     1321 agttctccat ccacgacgcg tggctggcga ccgggtcgat gtcctgcacc ttcgcccaca
     1381 ccttcggcgc gttgtaccag ttgtggaact tcacgcaggt gcccggatgg tccgccatcc
     1441 actccgagag cagcgctccc gccggcgcgt tgccgccgtg accgttcacg atcaggatgc
     1501 gccggaaccc gctccgccgc aggctgtcga gcacgtcgcg gaccagcgcg atcagcgtct
     1561 cgatccgcag gcagatcgtc cccggatacg ccgtgaagta cggcgtgtat ccgtaattga
     1621 gcgtcgggaa caccggcacc cccagcggct ccgccgcctc gaccgacacg cgctccgcca
     1681 ggacaaggtc ggtgccgagg ctcagcccgg cgtgctgctc cgtcgagccg atcgggacca
     1741 ccgcgcggtc gtcgtcccgc aggtaggcct cgacctgcct ccagttcatc tcggtgatct
     1801 tcatgggcgg tggccgtatc ctgtcaggcc ggctcgcggt tgaacagcgg ccaccgaccg
     1861 gagccgaccg cgatcgcgac gcgctgacaa gacctggaga taccttgggt tcgcccgccg
     1921 gcgacggccg gcgcaggtcc gccggaaacg cggtgttatc cggtgaaatg ttcatcttcc
     1981 ggcaaccgcg ttatctgtga tgaacccgtg tccgatcgcg gacgatagaa ggcatcgaaa
     2041 tgaggagggc tatgtatccg tcgagagttc gtgaaggtgc gctgctcgca gcgctggcag
     2101 ccggtctgta cgtcgaggcg tcctgcgggc cgaagccccc ggggaccggg ggacagcccg
     2161 cggtgaccgc gccgtcgccg tgcgcgcttc ccacggccat ggccggcagc aacgagcaga
     2221 ccgcctggca gctgttcgtg gccgcgagct gcccggtcgg cggcaagctc gcgtgggaga
     2281 cctggaccga gcagacctgc ttcaacaagc ccgacagccc cggctgcggc ccgcgcgccg
     2341 gcgccgccgt ggacgcggcc cgtcaccttc acggcagccc gctcggggag ggccgccggc
     2401 gagcccggtt gaagatggcg agcgccacgc cagggccgtg cagcgacatg acgacgaccg
     2461 ccaacgcccc ggcggagctg aggccgttcg tgcccaagaa cctgaccgcg aacgccaagt
     2521 tctgcgagga ggtgttcgtc aacgagcccg aggccgcgtt cgtccgggcg ccgtcccgcg
     2581 gcgcgaccct gaccaacttg acggcgcagg tgtcgtacgc gaaatttggc gcgatctcgt
     2641 ttccgacccc gtcggtcgag atcaaggccg actggctgcc ggcgacgtcg ctgaacaacg
     2701 cattcaactg cacgaccaac ccacccgacg gggtctacgt cgagaccatc tcggggacgt
     2761 gctacgcgct ggtcgggatc catgtctcgt ccaagctgtt gccgaactgg ctgtgggcga
     2821 ccttcgagcc ccagaacacc gtcaccaacc cgaaccgctg caatcccgat ctgtacagcg
     2881 catgcaacga cccctggggt tcgagccccc cggtgagcac cggccaggcc acccagccga
     2941 cccccgcgct cgcggcgctg atgagccagg ccaagctcgc cccgcagctg ctcaactatc
     3001 gcctggtcgc cgcccagtcc ctctacgtgg acgcgaacaa caacccgatc cagctcggca
     3061 actcgttcgt cgagttcaac gcggaggtcc cgcctcacca ggcgtcgtgc atcacctgcc
     3121 attcgtacgc gatggtgagc accaccgcga acgagaatcc gaacttcggc aacttcccgg
     3181 gcacaccgcc gatcgggatg ccgggaacgg cgccgctgcc ggcgcagggc ggcgggacct
     3241 gggtcacgca ggacttctcg tggatgctcg gcatcatgcc gcagacgccg caccacgagt
     3301 agcgcgggcc ggtcagctct cgcccgagcg aggaagctcg acgatcagcg ccgctccgtg
     3361 gggcgcgaca tcctcgatcc ggatccggcc accgtggagc atgaccagct cgtgcgccac
     3421 gtagagcccg agcccgagcg atccgctctt gccggtgccc tgaataaacc gttcgaacag
     3481 ccgctcgcgc agctcgaccg ggatccccgg tccctggtcg cgcaccgcga gatcaaccac
     3541 ctgcgccgtc gcggacagct ccaccgtgac cgtcccggcg gatggcgagt gccggatcgc
     3601 attctcgatc aagttgccga cgacctgccc gaaccggacg cgatccgccg cgaccaccgc
     3661 gtcgcccagc cggtcgacga gctcgaggcc gacgcccgca cggtcggcga tcggcgtgta
     3721 gatctcgagc gcatcgcgtg cgattgcgcg gatcgacacg ggtcgcgcgt cgaggatcac
     3781 ccgtccgctc tcggtccgcg cggcctccag gaccttctcg atgagctcca ccacctgggc
     3841 tgcgctgcgg tggatgcgcc cgcgcgcctg gttccacagc tcggtggacc tgctcgcatc
     3901 cgcgcgctcc atgacctcga ggctcagcgc gatcccctgg aggccccccc tcacctcgtg
     3961 ggcgagcatc gagacgaagt gctcgcgcag gtcggccagc gactcgaaac gcgttcgcgc
     4021 cgcctggatc tcggccgact ggcgcgacag ctcgcgggtg agattcgcga ggtgattgtt
     4081 ggcctggagg agctgctgcg ccatcgcctc cgagtactcc gcaccggagt gcgccacgag
     4141 gaccaaccgc gtgtccggcc cgagcgggag cgcgaccagg cgaaccggct gtggatcgcg
     4201 cccgccccgc tgcgcctgga cctcgcacga cgcaggtgcc gcggccagcg cgccggtcaa
     4261 cttgcttcgc gagtctgccg tgaacagcgc ctcgatgaac tggtcgacgt cgccatcgag
     4321 cagctcgcgt gccgcttcgt tgctcgacgc gatgatgcca tcgcgcacga cgagcaccgg
     4381 gatcgcgacg cggcgatcga gcaggagccc cacgacgtcg ac
//
LOCUS       DASVOR010001377         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_218777,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001377 DASVOR010000000
VERSION     DASVOR010001377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218777"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            505..1530
                     /locus_tag="VF516_26210"
     CDS             505..1530
                     /locus_tag="VF516_26210"
                     /inference="COORDINATES: protein motif:HMM:NF018378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucuronyl C5-epimerase family protein"
                     /protein_id="HEX8111261.1"
                     /translation="MERVSDVLFRVARWRRQLRLIAEDPLTLDPRIDPHAQQLGFTHV
                     YVAAEAGETERWLTSPVYTTEAARRDPIDVCHLAMDRFHVFLEHGDTAARRDFLAVTR
                     SLLEVGRTVELGGRTCFVMPHFDQVEGYARHGTPWVNAMVQGWVGALFMRAHQATGEE
                     RFAEAAVRAVGAFFVPVAHGGVRDLERNGRVFYEKYALPGQTRHVLNGFMSALLGLWD
                     VARASGDRDARLAFEEGVASLDDVVLDAYDNGYTTLYEQRSDRRATPSSVFYTWVHAR
                     QIASIARITREPRLRAWAGAWRAYSRRIDHRARTTLDTMGFRARSLPRYLGLDRPQDL
                     PRLSRPR"
     gene            2171..2305
                     /locus_tag="VF516_26215"
     CDS             2171..2305
                     /locus_tag="VF516_26215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111262.1"
                     /translation="MCWKLDLLALAGALPLDRLSLAVSTEAIAPALAAAGQHDVLTCR
                     "
     gene            2500..3411
                     /locus_tag="VF516_26220"
     CDS             2500..3411
                     /locus_tag="VF516_26220"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEX8111263.1"
                     /translation="MRPSTRALSVAALVLTSFVATAAAQRPVKVYISADMEGVTGVVS
                     ADQLGPASFEYAQARQWMTEEVLAAIQGAREAGATEFVVSDSHGNGESLLIDRFPSDI
                     LVTIVRSFPRPLAMMEGIDASFAAVIFIGYHTSTSSLTGVRAHTISSALLTRVAINGT
                     SMSEAGINAAIAAQFGVPVVMITGDDQIVAESKQRLGPIEGVVVKRAVGFHSAATLTP
                     AAGQALIRQRAKIAIGRRGEMRPYALAKPLTLEVSLKNYRPVELLGYLREVQRVDSHT
                     VRYAAKDMLEVSRFLEFVTNYDPTLAP"
     gene            complement(3528..>3760)
                     /locus_tag="VF516_26225"
     CDS             complement(3528..>3760)
                     /locus_tag="VF516_26225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111264.1"
                     /translation="TSPAVAMISSFDTCFAPNTSIAASVFGGGAGGAGAGLSAAASGA
                     GGLAALLVSAGAASGLAAVSGGFGCVGTAAGG"
BASE COUNT          595 a         1263 c         1231 g          671 t
ORIGIN      
        1 tgccaggctg gaagccgccc gcgaggttcg gcgcctgcgc cgcgccgagc tccaccagga
       61 ggcggcatga cccttctggt tccctgcttc aggctctgta accttcaccc agtatcgtca
      121 cgttcacgcc gaaccagtac agatcgagct ggtgtcggag ctcgacgccg gcagcacgtt
      181 caccgtcgtc cttcccccgc caatctcatg gaagacggtg gcgtcgctgc gcgcgctggc
      241 agcgtccggc atggtggcaa ggtagaccac ggcggcgggc ggctgtcagc cacgaggagt
      301 gcactgcccg tacaccgccc gagacgtggc agcgtgtggc gggatcgcgc ggacctgccg
      361 tcagttcatc gccggcgacg ttgctcgtgt ctcactgcgc gccggcgcac acacggacac
      421 tgcgagattg tcggtgactc agaaatcgcc cggatgcatg ctgaggcccg gagaacgagc
      481 atgagaatgg ttcccgaggt cgtgatggaa cgggtctctg atgtgctgtt ccgggtggct
      541 cgatggcggc gacagctccg gctgattgca gaggacccgc tcacactcga tccgcgtatc
      601 gatccgcacg cccagcaact ggggttcacg cacgtctacg ttgccgcaga ggcgggtgag
      661 accgagcgct ggctgacctc gccggtgtac accaccgagg ccgcccggcg agatccgatc
      721 gatgtctgcc atctcgcgat ggatcgattc catgtcttcc tcgagcatgg tgacaccgca
      781 gcacgccgcg acttcctcgc ggtgacgcgg agcttgctcg aggtcggtcg caccgtcgag
      841 ctcggtgggc gtacctgctt cgtgatgccg cacttcgatc aggtcgaggg ctatgcgcga
      901 cacggcacac cgtgggtcaa cgcgatggtg cagggctggg tcggcgcgct gttcatgcgt
      961 gcccaccagg ccacgggcga ggagcgcttc gcggaggcgg cggtgcgcgc agtcggcgcg
     1021 ttcttcgtgc cggtcgccca cggcggggtc cgcgatctcg agcgcaacgg ccgtgtgttc
     1081 tacgagaagt acgcgcttcc cggacagacg cgccatgtcc tcaacggctt catgtcggcg
     1141 ctgctcgggc tctgggatgt cgcgcgcgcg agtggtgatc gcgatgcacg gttggcgttc
     1201 gaggagggcg tggcgtcgct cgatgacgtc gtgctcgacg cgtacgacaa cggctacacc
     1261 acgctctacg aacagcgcag cgatcgccgc gcgacgccat cgagcgtgtt ctatacctgg
     1321 gttcacgctc gccagatcgc atcgatcgcg cgcatcacca gggaaccgcg gctccgcgcg
     1381 tgggcgggcg cttggcgcgc gtactcgcgg cgcatcgacc atcgcgcgcg gaccacgctc
     1441 gatacgatgg ggttccgggc ccgcagcttg ccaaggtacc tcgggctcga tcgtccgcag
     1501 gacctgccac gcctcagccg gcctcgttga tccgaggcct ccgactacct tcacgcacct
     1561 ggcccgatat cgacgcgatg aggccgccgc gatgagtcgc gagccagccg atcgtttcca
     1621 tcgaccgcag actgagagtc gatgccagag cgatgcgatt gacggatgac aatcgatccc
     1681 ggaactctcc cggcatgatg aaaacatgtt cgatgatcgg attctcatga tctcgagtgt
     1741 gatgggctgc gttgaaaacg agccagcaac caagccgaga cctcggagct caggtccggg
     1801 acctgcgaca acaccaccag caccatgctc tcttgtcgag ggaaaccgtt ctatttcacc
     1861 ttcgccgatg gttcctactg ctccccgcat acaacaggaa cgatttgcac gttgctcgct
     1921 tctgacttct gctcctttga atcttcgggc ctctgccaag gtaagcgcca ggggacccag
     1981 tgcgggactg acggggaggg cttcccgaag tcttgccagg atggaggcgc cgctggtgcc
     2041 cattcatgtt tctgtagctc gtgaggaacc ggacgcgcgt cgatcgcaga ccgtgtcaag
     2101 ctccgctcga acgtagccgg gtgcagctcg gacagatgct tccatgcgcc gatcgccggc
     2161 gatgcgcttc gtgtgctgga agctcgactt gctcgcgctg gctggggctt tgcccctcga
     2221 tcgcctgtct ctcgcagtga gcaccgaggc gatcgcgcct gcgctcgccg ccgccggtca
     2281 gcacgacgtg ctcacctgcc gctgatggga gccacctggc ggccctgcag cagaccctgg
     2341 gcggccggtc gtgtacgttc cgctgcacga cggtatccag ctctgctgcc tccgcggcgg
     2401 gctgctgttc ggcctcgcgc tcgctcgtcg cgcgctggcg ctgtccctcg ccgatatcgc
     2461 ttcttgcgac cggccgcgat tcgggccatc gtctgccgcg tgcgcccctc gactcgagca
     2521 ttgtctgtcg ctgcactcgt tctgacgtca ttcgttgcca ccgctgccgc ccagcgtccg
     2581 gtcaaggtct atatctcggc cgatatggag ggcgtcacgg gtgtcgtgtc ggccgaccaa
     2641 ctcggccccg cgagcttcga atatgcccag gcccgccagt ggatgaccga ggaagttctc
     2701 gccgcgatcc agggcgctcg cgaggcgggg gcgacggagt tcgtcgtgag cgactcgcac
     2761 ggcaacggtg aatcgttgct catcgaccga ttcccgtcgg acatcctcgt cacgatcgtg
     2821 cgatcgtttc cgcggccgct cgcgatgatg gaagggatcg acgcgagctt cgccgcggtg
     2881 atcttcatcg gctaccacac ctcgacgtcg agcctgaccg gcgtccgggc tcatacgatc
     2941 tcgagcgcgc tgctcacgcg ggtcgcgatc aacggcacgt cgatgtcgga ggcggggatc
     3001 aatgccgcaa tcgccgcgca gttcggcgtg ccggtggtca tgatcacggg cgacgaccag
     3061 atcgtcgctg aatcgaagca gcgcctcggc ccgatcgaag gcgtggtcgt gaagcgggcg
     3121 gtcggctttc attcggcggc gacgctgacg cccgcggccg ggcaggcgct catccgccag
     3181 cgcgccaaga tcgcaatcgg acggcgcggc gagatgcgcc cgtacgcgct ggcgaagccg
     3241 ctcacgctgg aggtctcgct caagaactat cgtccggtcg agctgctcgg ctatctgcgc
     3301 gaggtacagc gcgtggactc gcacaccgta cgctacgcgg cgaaggacat gctggaggtg
     3361 tcgcgattcc tcgagttcgt gacgaactac gaccccacgc tcgctccgtg acccggtcgc
     3421 gaccggcgaa tgccgtcctt tttgctggcg ctgccgtgta aactacagtt gtagtagcac
     3481 atcatgaagc actggatcgc ctgctggacg ctcgtcgccg cttgctctca tcctcccgcc
     3541 gccgtgccga cacagccgaa accgcccgag accgcagcca ggccggaggc cgcgcctgca
     3601 gagacgagca gcgcagccaa gccgcctgcg ccggacgccg ccgccgagag ccccgcgcct
     3661 gcgccgccgg ctccgcctcc gaagacgctg gccgcgatcg acgtattcgg cgcgaagcac
     3721 gtgtcgaacg aggagatcat cgccacggcg gggctcgtgg
//
LOCUS       DASVOR010001378         2370 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_452345,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001378 DASVOR010000000
VERSION     DASVOR010001378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2370
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452345"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2347)
                     /locus_tag="VF516_26230"
     CDS             complement(<1..2347)
                     /locus_tag="VF516_26230"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8111265.1"
                     /translation="MNLSAPFIRRPKATSLLAAALLLAGAAAFTQLPVSPLPKVDYPT
                     INVSANLPGASPVTMATAVAMPLERRFGRIAGVSEITSQSSLGTASVTVQFDMDRDVE
                     GAARDVQAAINAAGGDLPANLPNRPNDRKVNPSDAPILIISLRSKSLPLPTVFEAANT
                     VLAQKLAQVPGVGQVGVGGGVQPAVRVQVDPAVLAGLGIGLEEVRNALASATSNQPKG
                     AVGAQQWHTIGVDDQLLDAEAWKNVIIHWTEPGGAPAIDPTAAAATANGAPAIAAVNS
                     TFSAGSGSGSAAVVRGDVGAGIRLGDIATVKDDVENQRVAGWYDGERTVMVYVKRQPG
                     ANILEVIDRIKALLPQLTHEISPAIDVQVAIDRSMSIRASVHDVERSLMISILLVVVV
                     VFLFLRSARATAIPSVAVPLSLIATFGVMYLCGFSVDNLSLMALTIATGFVVDDAIVV
                     TENVTRHIEQGAPPRLAALAGARQVGFTIISITASLLAVFIPLLFMGGIVGRLFREFA
                     VTLAIAISLSAVISLTLTPMMTSELLRPHRAAGGLGRWLERGLHGLTAGYARALRFVL
                     RHQLLIGVTTIATILTTCGLYTRVPPGLFPQQDTGQLMGSSLGPQDISYPAMKARQEQ
                     LNAIVMQDPDVAHVVSNIGGWGSSTGNQGNMFIQLRPLPERKSTVDEVIARLRPKLAR
                     VQGIQLFLQAVQDVRVGGRGARTQYQYTLEDADLAELDTWAPRLAAALRKLPEIKDVN
                     SDQQNAGLQLTVDIDRDTAARFGISTATIDNTLYDAFGQRQV"
BASE COUNT          336 a          819 c          866 g          349 t
ORIGIN      
        1 cgacctggcg ctggccgaac gcgtcgtaca gcgtgttgtc gatcgtcgcg gtcgagatcc
       61 cgaaccgcgc cgcggtgtcg cgatcgatgt cgacggtgag ctgcagcccg gcgttctgct
      121 gatcgctgtt gacgtccttg atctcgggca gcttgcgcag cgccgcggcg agccggggcg
      181 cccaggtatc gagctcggcg agatcggcgt cctcgagcgt gtactggtac tgggtgcgcg
      241 cgccgcggcc gccgacccgg acgtcctgca ccgcctgcag gaacagctgg atgccctgga
      301 cccgcgcgag cttgggccgc agccgcgcga tcacctcgtc gacggtcgac ttgcgctcgg
      361 gcagcggcct gagctggatg aacatgttgc cctggttgcc ggtgctcgag ccccagccgc
      421 cgatgttgga caccacgtgg gcgacgtcgg ggtcctgcat cacgatcgcg ttgagctgct
      481 cctggcgggc cttcatcgcg gggtacgaga tgtcctgggg gccgagcgac gagcccatca
      541 gctggccggt gtcctgctgc gggaacagcc cgggcgggac cctggtgtac agcccgcacg
      601 tggtgaggat cgtggcgatc gtggtcacgc cgatcagcag ctggtggcgc agcacgaacc
      661 ggagcgcgcg tgcgtagccg gcggtcaggc cgtgcagtcc gcgctcgagc cagcgcccga
      721 gccctccggc cgcccggtgt ggccggagca gctcgctggt catcatcggg gtcagggtca
      781 gcgagatcac cgcggacagc gagatcgcga tcgcgagggt gaccgcgaac tcgcggaaca
      841 gccggccgac gatgccgccc atgaacaaga gcgggatgaa caccgcgagc agcgacgcgg
      901 tgatgctgat gatggtgaac ccgacctggc gcgccccggc gagcgcggcc aggcgcggtg
      961 gcgcgccctg ctcgatgtgg cgggtcacgt tctcggtgac cacgatcgcg tcgtcgacca
     1021 cgaacccggt cgcgatggtc agcgccatca gcgacaggtt gtcgacgctg aacccgcaca
     1081 ggtacatcac gccgaacgtc gcgatcagcg acagcggcac cgcgacgctc gggatcgcgg
     1141 tcgcccgcgc gctgcgcagg aacaggaaca cgaccaccac gaccagcagg atgctgatca
     1201 tcagcgagcg ctcgacatcg tgcaccgacg cgcggatcga catcgaacga tcgatcgcga
     1261 cctggacatc gatcgccggc gagatctcgt gggtgagctg gggcagcagc gccttgatcc
     1321 ggtcgatcac ctcgaggatg ttcgccccgg gctggcgctt gacgtacacc atcacggtgc
     1381 gttcgccgtc gtaccagccg gcgacccgct ggttctcgac gtcgtccttg acggtcgcga
     1441 tgtcgcccag ccggatcccg gcgccgacgt caccgcgcac cacggccgcc gagccggagc
     1501 ccgagccggc cgagaacgtg ctgttgacgg ccgcgatcgc cggcgcgccg ttcgccgtgg
     1561 cggccgccgc cgtcgggtcg atcgcgggcg cgccgccggg ctcggtccag tggatgatga
     1621 cgttcttcca ggcctcggca tcgagcagct gatcgtcgac gccgatcgtg tgccactgct
     1681 gggcgccgac cgcgcccttg ggctggttcg aggtcgcgct ggccagcgcg ttgcgcacct
     1741 cctcgaggcc gatcccgagc ccggcgagca cggcggggtc gacctggacg cgcacggcgg
     1801 gctggacgcc gccgccgacg ccgacctggc cgacgcccgg cacctgcgcg agcttctggg
     1861 ccagcacggt gttggcggcc tcgaacacgg tcggcagcgg cagcgacttc gatcgcagcg
     1921 agatgatcag gatcggcgca tcggacgggt tgaccttgcg gtcgttgggc cggttcggca
     1981 ggttggccgg caggtcgccg ccggccgcgt tgatcgcggc ctggacgtcg cgcgccgcgc
     2041 cctcgacgtc gcggtccatg tcgaactgca ccgtgacgct cgcggtgccg agcgagctct
     2101 gcgacgtgat ctcgctgacc ccggcgatcc ggccgaaccg gcgctcgagc ggcatcgcga
     2161 ccgcggtcgc catcgtgacc gggctcgcgc ccggcaggtt cgcgctgacg ttgatcgtcg
     2221 ggtagtcgac cttgggcagc ggcgacaccg gcagctgggt gaacgccgcg gcgcccgcga
     2281 gcagcagcgc ggccgcgagc agggaggtcg ccttgggccg gcggatgaac ggcgccgaga
     2341 gattcatcct gccccctggc cgtctggcat
//
LOCUS       DASVOR010001379         3748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_364852,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001379 DASVOR010000000
VERSION     DASVOR010001379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_364852"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..1312
                     /locus_tag="VF516_26235"
     CDS             149..1312
                     /locus_tag="VF516_26235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111266.1"
                     /translation="MAEIVLHAIAHSYQLARGGRERRRGAAPSGALRDVELRWRDGGA
                     YALLGPSGCGKTTLLGIISGLIQPTRGTVHYGGRDVTDASSRDRNIAQVFQFPVVYDT
                     MTVRDNLAFPLRNRGLGAAEIDRRVGRIAEALELTELLALRATGLTADQKQILALGRG
                     LVRDDVAAILFDEPLTVIDPHRKWLLRRKLKEIHRQFRHTLIYVTHDQTEALTFADEV
                     VVMQAGQVLQQGTPEQLFLQPAHTYVGYFIGSPGMNVLPCSIRHDGRHDGGARAQIGD
                     HAIALPPGVDPALDGDGFELGIRPEHIAVRAGAGLPLVVTAIEDHGRFRIVSARLGDQ
                     PLKLRLGPAGAHDAAPPVVGQTLHVEFPPERICLYRDGRIAAPVAPAARGGAA"
     gene            1309..2175
                     /locus_tag="VF516_26240"
     CDS             1309..2175
                     /locus_tag="VF516_26240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8111267.1"
                     /translation="MIEKTRTGRAWLLILPMLVLVSFSAIVPLMTVVNYSVQDILGPS
                     TKVYVGFEWFRKVLRDPDLHAAFLRQLLFSAAVLAIEVPLGLGVSLIMPRRGWAVSAA
                     LVVLALPLLIPWNVVGTIWQIFGRSDIGMGGAALVKLGLHYNYTLDRVDAWLTVLVMD
                     VWHWTPLIALLCYSGLRAIPPAYYQAAAIDGASTWSVFRYIQLPKLGNVLAIAVLLRF
                     MDSFMIYTEPFVLTGGGPGNSTTFLSQYLTTLAVGQFDLGPAAAFSLIYFVFILFFCY
                     VFYTVMNRVGRG"
     gene            2203..>3748
                     /locus_tag="VF516_26245"
     CDS             2203..>3748
                     /locus_tag="VF516_26245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8111268.1"
                     /translation="MRSPCHRASGIAAIAVAAALLAGGCGKKKQDAGGGSGSALEPPT
                     VDLNAAIGKWVREFQPSTLTPEQQVAELKWFREAAKPFAGMEIKVVSETIDTHVYESK
                     TLAPAFTQLTGIKVTHDIIQEGDLIEKLQTQMQSGENIYDMYINDTDLIGTHYRYGDA
                     IALSDFMAGEGKDVTLPTLDVDDFIGKSFGTAPDGKLYQLPDQQFANLYWFRYDWFQR
                     DDLKQKFKAKYGYELGVPVNWSAYEDIADFFTNDVKEIDGHRVWGHMDYGKKDPSLGW
                     RFTDAWLSMAGAGSKGLPNGLPVDEWGIRMEDCHPVGSSVSRGGEANSPAAVYALTKY
                     IDWLKKYAPPEAAGMTFSEAGPVPGQGNIAQQIFWYSAFTAALSKPGLPVVNADGTPK
                     WRMAPSPHGPYWEKGMKLGYQDAGAWTMEANVPLQRRKAAWLYAQFTISKTVTLKKFF
                     EGLTPIRKSDIYSDPVTKAAPKYGGLVEFYRSSARELWTPTGNNVPDYPKLAQLWWPN
                     VSEAVSG"
BASE COUNT          588 a         1313 c         1267 g          580 t
ORIGIN      
        1 gcacgacggc gtgctgcacc accacctcga cgacgagctc acgttcttcg tcgaccccga
       61 ccggctgctc gcgttcgaca agggcggccc ggagccgctg gtcgcgtcct atcaccccgc
      121 ccggcgcgcc gccgcgggag cgtgagccat ggccgagatc gtcctccacg ccatcgctca
      181 cagctaccag ctcgctcgcg gcggccgcga gcgccggcgc ggggcggcgc cgagcggggc
      241 gctgcgcgac gtcgagctgc gctggcgcga cggcggggcc tacgcgctgc tcgggcccag
      301 cggctgcggc aagaccacgc tgctcggcat catctcgggg ctgatccagc cgacgcgcgg
      361 caccgtgcac tacggcggcc gcgacgtgac cgacgcgtcg agccgcgatc gcaacatcgc
      421 gcaggtgttc cagttccccg tggtctacga cacgatgaca gtgcgggaca acctcgcgtt
      481 cccgctgcgc aaccggggcc ttggcgccgc cgagatcgat cgccgggtcg gccggatcgc
      541 cgaggcgctc gagctgaccg agctgctggc gctgcgcgcg accgggctga ccgccgacca
      601 gaagcagatc ctcgcgctcg gccgcgggct ggtgcgcgac gacgtcgcgg cgatcctgtt
      661 cgacgagccg ttgaccgtga tcgatccgca ccgcaagtgg ctgctgcggc gcaagctcaa
      721 ggagatccat cgccagttcc gccacacgct gatctacgtc acccacgatc agaccgaggc
      781 gctgacgttc gccgacgagg tcgtggtgat gcaggccggc caggtgctgc agcagggcac
      841 cccggagcag ctgttcctgc agccggccca cacctacgtc ggctacttca tcggctcgcc
      901 cgggatgaac gtcctgccgt gctcgatccg acacgatggc cggcacgacg gcggcgcccg
      961 cgcgcagatc ggcgaccacg cgatcgcgct gccacccggg gtcgatcccg cgctcgacgg
     1021 ggacggcttc gagctcggca tccggccgga gcacatcgcc gtgcgcgccg gggccggcct
     1081 gccgctggtg gtcaccgcga tcgaggacca cggccggttc cggatcgtgt cggctcggct
     1141 tggcgaccag ccgctcaagc tccggctcgg ccccgccggg gcgcacgacg ccgctccgcc
     1201 ggtcgtcggc cagacgctgc acgtcgagtt cccgcccgag cggatctgcc tgtaccgcga
     1261 cggccggatc gcagcgccgg tggcgcccgc cgcgcgcggg ggggccgcat gatcgagaag
     1321 acccgcaccg gccgggcctg gctcttgatc ctgccgatgc tggtgctggt gtcgttcagc
     1381 gcgatcgtcc cgctgatgac cgtggtcaac tactcggtgc aggacatcct cgggcccagc
     1441 accaaggtct acgtcggctt cgagtggttc cggaaggtgc tgcgcgatcc tgatctgcac
     1501 gcggcgttcc tccgccagct gctgttctcg gccgcggtgc tcgcgatcga ggtgccgctc
     1561 gggctgggcg tcagcctgat catgccgcgg cggggctggg cggtctcggc ggccctggtc
     1621 gtgctcgcgc tgccgctctt gatcccgtgg aacgtcgtcg ggacgatctg gcagatcttc
     1681 ggccgcagcg acatcggcat gggcggcgcg gcgctggtca agctcgggct gcactacaac
     1741 tacacgctcg accgggtcga cgcgtggctg accgtgctgg tcatggacgt ctggcactgg
     1801 acgccgctga tcgcgctctt gtgctactcc gggctgcgcg ccattccgcc ggcgtactac
     1861 caggcggcgg cgatcgacgg cgcgtcgacc tggtcggtgt ttcgctacat ccagctgcct
     1921 aagctcggca acgtgctggc gatcgccgtg ctgctgcggt tcatggacag cttcatgatc
     1981 tacaccgagc cgttcgtgct gaccggcggc ggcccgggca acagcaccac attcctcagc
     2041 cagtacttga caacgctcgc cgtcgggcag ttcgatctcg ggccggcggc ggcgttctcg
     2101 ctgatctact tcgtgttcat cctgttcttc tgttacgtgt tctacaccgt catgaatcgc
     2161 gtcggacgag gataaatcat catgcataca cgatcatcga tattgcgcag tccgtgtcac
     2221 cgcgcctccg ggatcgccgc gatcgcggtg gcggcggcgc tgctcgccgg cggttgcggc
     2281 aagaagaagc aggacgccgg cggcggcagc ggctcggcgc tcgagccgcc caccgtcgac
     2341 ctcaacgccg cgatcgggaa gtgggtccgg gagttccagc ccagcacgct gacccccgag
     2401 cagcaggtcg ccgagctcaa gtggttccgc gaggcggcca agccgttcgc cgggatggag
     2461 atcaaggtcg tctcggagac catcgacacc cacgtctacg agtccaagac gctggcgccc
     2521 gcgttcaccc agctcaccgg gatcaaggtc acccacgaca tcatccagga gggtgacctg
     2581 atcgagaagc tgcagaccca gatgcagtcg ggcgagaaca tctatgacat gtacatcaac
     2641 gacaccgacc tgatcggcac ccactatcgc tacggcgatg cgatcgcgct cagcgacttc
     2701 atggccggcg agggcaagga cgtcacgctg ccgacgctcg atgtcgacga cttcatcggc
     2761 aagagcttcg gcaccgcgcc cgacggcaag ctgtaccagc tccccgatca gcagttcgcg
     2821 aacctgtact ggtttcgcta cgactggttc cagcgcgacg atctcaagca gaagttcaag
     2881 gccaagtacg gctacgagct cggcgtgccg gtcaactggt cggcgtacga ggacatcgcc
     2941 gacttcttca ccaacgacgt caaggagatc gacggccacc gggtgtgggg ccacatggac
     3001 tacggcaaga aggacccgtc gctgggctgg cggttcaccg acgcgtggct gtcgatggcg
     3061 ggcgccggca gcaagggcct gcccaacggc ctgccggtcg acgagtgggg catccgcatg
     3121 gaggactgcc atccggtcgg ctcgtcggtt tcccgcggcg gcgaggccaa cagcccggcc
     3181 gcggtgtacg cgctgaccaa gtacatcgac tggctcaaga agtacgcgcc gcccgaggcc
     3241 gccggcatga cgttctccga ggccggcccg gtccccggcc agggcaacat cgcgcagcag
     3301 atcttctggt actcggcgtt caccgccgcg ctcagcaagc ccggcctgcc ggtggtcaac
     3361 gccgacggca cgccgaagtg gcggatggcg ccgtcgccgc acggcccgta ctgggagaag
     3421 ggcatgaagc tcggctacca ggacgccggc gcgtggacga tggaggccaa cgtgccgctg
     3481 cagcgccgca aggcggcgtg gctgtacgcg cagttcacga tctcgaagac ggtcacgctc
     3541 aagaagttct tcgagggcct gaccccgatc cgcaagtcgg acatctactc cgatccggtc
     3601 accaaggcgg cgccgaagta cggcgggctg gtcgagttct accgcagctc ggcgcgcgag
     3661 ctgtggacgc cgaccggcaa caacgtcccc gactacccca agctggcgca gctgtggtgg
     3721 cccaacgtca gcgaggcagt gtccggca
//
LOCUS       DASVOR010001380         8184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_189736,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001380 DASVOR010000000
VERSION     DASVOR010001380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8184
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_189736"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..2059
                     /locus_tag="VF516_26250"
     CDS             257..2059
                     /locus_tag="VF516_26250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111269.1"
                     /translation="MRAVRHLVLVVVVVHASPSFAQSLPPDAKPTCTASIASWFASGS
                     VTLNGAVNPANGLALDTSNNCNFYLWSEQMFLWMTSPAASIYGGNGRVFQSPVFFGVE
                     NDQLVPQFTMPPFIPPIGVPPDAVKPPSSKLPPLTGVKLMKQLAAAVRPAKRGPHGLP
                     VVIDRKGHAYEVVDAQRSARGRPFVLSRAGAHTEVSRVTVDADHHVTFHDAAGRVIPQ
                     PRPVLEARLRGAQVVQRFFTSPNEAVFVDEAGSALDVSPEQAGRIGAVLLAHSGAVVY
                     YTIAVNDVYAWFRTGVANGAINTNNQFPTTQSDLDQIVNYAAQYGVTFPDANALAIEA
                     KLSWVDASTLPNPGDYITMPAVVPVYSTASSQSWPQTGTKPVTLALLGVHVVGSSIGH
                     PEMLWATFEHFGNTPNASYQYVNKAGATTTVPQNTSGTWTLTSSSSSGPFNVAHANYL
                     SAPAITAQSGQTISPSDTLRQNAFGVAPVGIPNQNDATPAIANTEVLALNNSVLGQLV
                     TGDVRKNYYFLGTTWTFGGAPPTTPFPVGGNEIGTSYLSNSTMETYQQWMSTSSPGNN
                     CLDCHQSSSPSITNTDVSHIFGSLAPLFTMPPKR"
     gene            complement(2109..3110)
                     /locus_tag="VF516_26255"
     CDS             complement(2109..3110)
                     /locus_tag="VF516_26255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111270.1"
                     /translation="MNVLVIGGNRFLGRGLVWRLLCGGHRVTLVNRGRLADPFGDRVE
                     RIHVDRSSDAFDRALAGRTFDRAVDFAGFTADDAVRSVRVLGGRVGHYVFVSTGQVYL
                     VREGCPVPSRETDYDGPTMAAPPSPADHDDWAYGIGKRGAEDVLAGAAALPSTRLRIP
                     MVNGELDYQRRIESYLWRLLDGGPLLVARAGALRRPVYGGAVVRAIAKLLEAPPLPGQ
                     AFNLAQPEPLTVRALIERIAARAGARPEIVERSAEAIEAAGLSVCDASPFSSAWSSLL
                     DPSRAVAELGFEHPPLDAYLDATIASLLATWPPAPPPGYAQRDRELAEAARRNGATV"
     gene            3321..3629
                     /locus_tag="VF516_26260"
     CDS             3321..3629
                     /locus_tag="VF516_26260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111271.1"
                     /translation="MASSAASEGHRTPGTPGKATLTEALPVIAAPVQRKADPVSERFD
                     DTGVHQAAAAGVAGGGGQLPHVAETIRVYVSNKDTPRFAEYQKIVPHRFEILKKEYDK
                     "
     gene            3626..4072
                     /locus_tag="VF516_26265"
     CDS             3626..4072
                     /locus_tag="VF516_26265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111272.1"
                     /translation="MIRAAVACLAFGLAGCASGAHQEPPSNAATTGSPGPGPAGSENK
                     MTGAVSPAGDKHPGQIVYEDRVENRTWTRNAAEVSQAMAWVKVHDQWKPVVRIEITGG
                     GNTREMTSFGPGHEFLETTTAQLSGAPHGEALPTPVPTPTPIPTSK"
     gene            complement(4089..5477)
                     /locus_tag="VF516_26270"
     CDS             complement(4089..5477)
                     /locus_tag="VF516_26270"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8111273.1"
                     /translation="MNRRGLVALLFCACHTTSAPPPVAPSVATVTGHVAVDPAAGPAI
                     TRGALFVSWLTEAEKTAFDQGRPPIVLVRDLLTRGVVAPDVDAAQNAAFTVPAGRGRI
                     ALIAALDVSHVGLPAIQGEGKGTLVGATAVFDNGPAGAAAPVIALASRPSRERPEACQ
                     GDRLTLERIEAPEVAGTVGNPTSRRACVRVPRGYAEHPERHYPVIYALPGLLSTDAAA
                     VPGYLLDLQDTIVVGVDTSTRTGSTYLVDSPTSGKWDTFFTKDLIPYIDAHYRALPRR
                     EARAVVGHSTGGFNAVSYGLRHPELIGVIGASSPDGLDLPVWFGTGDVRPWLPGWARV
                     EHGLGGAGQFISYAADWSPSPSGYDWPFDASGHVIDSVLARWIANSPTTWVRDPARVA
                     ALEPLSGHIYLAVGDSDEFDLYPPTAAFSKTLTEAGIANELMVTHGGHANRVQHLAAI
                     ARFCAAKLEPAK"
     gene            complement(5490..7103)
                     /locus_tag="VF516_26275"
     CDS             complement(5490..7103)
                     /locus_tag="VF516_26275"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEX8111274.1"
                     /translation="MPQLPAELKWAQALGRDFTHGSTVQSFIDGRTALAAIHRAIQTT
                     HGSGDFIYLLAWWLDPDLPLVWSGDEPDPSSTLHEILPEKAKAGVQIRAMLWPNPSKL
                     FAPHDSPGELAAIVRQGIDPEEYYARILNDLPADPTQNRSAEIADLPRSHKRIVEWIN
                     SLPGAAAIDDGLTPRRSGCHHQKVLCVRGSEGLYALCGGVDLNPDRVAVVRHGRGEPL
                     HDVHCEVRGPAAVDLVQVFLQRWDSHPSGAHYDQTVGPVLGRTIAHGPDVPGAPGQIV
                     RVATTFNRVSPEDLKKLDRRACKVERGIRDGLLGAIASARGFIYFEDQYMTSLTVAEA
                     LGRAAKNLKYVLALITGSAISDLPQVWRRRKEFIACARSKGLGDKLHVYYLVDPVRPP
                     DAQENTYIHAKTWVFDDELAYVGSANVNNRGLSSDSEVGLFICDRTTSVCPPHGFAKD
                     LRIRLWARHLGMEVLDGCTGIDAWQSPPPSQTSASYRVRPYDPDGGKDHPILDAIPWS
                     IVDPDLEDLPECESGVDRARRLLHKAVES"
     gene            complement(7259..>8184)
                     /locus_tag="VF516_26280"
     CDS             complement(7259..>8184)
                     /locus_tag="VF516_26280"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111275.1"
                     /translation="VSRITRGKFQLILDDVDLRGPIEAAIESTAPLFAERGVTLEHSL
                     PRQAMPMRGDASRLVQVVVNLLSNAANYSPRGSTVRLVVTVEGDRGVLRVIDHGVGIE
                     SELQSKIFDLFVQSEQRLDRSRGGLGVGLSLAKNIVDLHGGTIEVHSEGPDTGSDFKV
                     SLPLSPRAVLHLGSDRSGDGRSGRCRIVLVDDQADSREMLRMLLESRDHLVIDVEDGP
                     SAIDVITREKPDVAFIDIGLPAMNGYEVAQRIRDRPGLDDVMLVALTGYGAPSDISAA
                     RAAGFDEHVIKPAELAKLERILATRKPSAPD"
BASE COUNT         1305 a         2800 c         2866 g         1213 t
ORIGIN      
        1 cgcgcggagc tcgagcggct cgccggcctg tatcgcgccg ccggccgcgc ggtgatctcg
       61 ccgggccgcc gcggctgacc gtgcgcggcg gtgatgcgct gtaatgcccg gtaattgatc
      121 cggcgctgcg cgcgaccgag agtgggtgtg tgtcgcgagc gcgaggtagc gcgagtctcg
      181 ggaggcgcgg ctgtcagcaa gccgaggtat ccgagggtcg cccgcgcctg cgtgtgaacg
      241 ggatcgagga ggaaacatgc gcgcggttcg tcatctggtt cttgtcgtcg tcgtggtcca
      301 tgcatcgccg tccttcgcac aatcgcttcc gccggatgcg aagccgacct gcacggcgag
      361 catcgcttcc tggtttgcgt cgggatcggt cacgctcaat ggagccgtca atcccgccaa
      421 cggcctcgcg ctcgacacgt cgaacaactg caacttctat ctgtggtccg agcagatgtt
      481 cctgtggatg acatcgcctg ccgcgtcgat ctacggcggc aacgggcgcg tcttccagtc
      541 gcccgtgttc ttcggcgtgg agaatgatca gctcgtcccg cagttcacga tgcctccgtt
      601 catcccgccg atcggcgtgc ccccggacgc ggtcaagccg ccgtcctcga agctgccgcc
      661 gctgaccggg gtgaagctca tgaagcagct ggcggcggcc gtgcgcccgg ccaagcgcgg
      721 cccgcacggc ctgccggtgg tcatcgaccg gaagggccat gcgtacgagg tcgtcgatgc
      781 gcagcggtcc gcgagaggcc ggccgttcgt gctgtcccgc gccggagcgc acaccgaggt
      841 gtcgagggtg accgtcgatg cggaccacca tgtgacgttc cacgacgcag ccgggagggt
      901 gatcccgcag ccgagaccgg tgctcgaggc gaggctgagg ggtgcgcagg tcgtgcagcg
      961 cttcttcacg tcgccgaacg aggcggtgtt cgtcgacgag gccggcagcg cgctcgacgt
     1021 ctctcccgag caggccggcc ggatcggcgc cgtcctgctc gcccacagcg gcgccgtcgt
     1081 gtactacacg atcgccgtca acgatgtgta cgcctggttc cgcaccggcg tggccaacgg
     1141 tgcgatcaac accaacaacc agttcccgac gacgcagtcg gatctcgacc agatcgtcaa
     1201 ctacgcggcc cagtacggcg tcacgttccc cgatgcgaac gccctggcca tcgaggccaa
     1261 gctgtcgtgg gtcgatgctt cgaccctgcc gaacccgggc gactacatca ccatgccggc
     1321 cgtggtcccc gtgtacagca ccgcgagctc gcagagctgg ccccagaccg gcacgaagcc
     1381 cgtgacgctg gccctgctcg gcgtccacgt cgtcggcagc agcatcggcc atccggagat
     1441 gctgtgggcc acgttcgagc acttcggcaa cacgccgaac gcgtcctatc agtacgtgaa
     1501 caaggccggc gccacgacca cggtgccgca gaacacgtcc gggacgtgga cgctcacgtc
     1561 gtcgagctcg agcggcccgt tcaacgtcgc gcatgccaac tatctgagcg cgcccgcgat
     1621 cacggcgcag tccggccaga cgatcagccc gagcgacacg ctgcgccaga acgcgttcgg
     1681 cgtcgcgccg gtcggcattc cgaaccagaa cgacgcgacg ccggcgatcg cgaacaccga
     1741 ggtgctggcg ctgaacaaca gcgtcctggg ccagctcgtg accggcgatg tccgcaagaa
     1801 ctactacttc ctcggcacga cctggacttt cggaggcgcg cccccgacca ctccgttccc
     1861 ggtcggcggc aacgagatcg ggacgagcta cctgtccaac agcacgatgg agacctacca
     1921 gcagtggatg agcacgtcga gtccgggcaa caactgcctg gactgccacc agtccagcag
     1981 cccgtcgatc acgaataccg atgtgagcca catctttggc tcgctggcgc cgctgttcac
     2041 gatgcccccg aagcgctgat gtcagcaaca aggggcacgc cgcggttctg atctccgagg
     2101 ttcgcgcgct acactgtcgc gccgttgcgc cgggcagcct cggcgagctc gcgatcgcgc
     2161 tgcgcgtagc ccggcggcgg cgccggcggc caggtcgcca gcaggctcgc gatcgtcgcg
     2221 tcgagatacg catcgagcgg cgggtgctcg aagccgagct cggccaccgc acgcgagggg
     2281 tcgagcagcg atgaccacgc gctcgagaac ggcgacgcgt cgcacaccga cagccccgcg
     2341 gcctcgattg cctcggcgga gcgctcgacg atctccggcc gggcgcccgc ccgcgcggcg
     2401 atccgttcga tcaatgcgcg caccgtcagg ggctcgggct gcgccaggtt gaacgcctgg
     2461 ccgggcaacg gtggcgcctc gagcaacttc gcgatcgcgc ggaccacggc gccgccgtac
     2521 accgggcgtc gcagcgcccc ggcgcgcgcc accagcagcg gcccgccgtc gagcaaccgc
     2581 cacaggtacg actcgatgcg ccgctggtag tcgagctcgc cgttgaccat cgggatacgc
     2641 agccgggtcg acggcagcgc cgcggcgccg gcgagcacgt cctcggcgcc gcgcttgccg
     2701 atgccgtagg cccagtcgtc gtggtcggcg ggcgacggcg gcgcagccat cgtcggtccg
     2761 tcgtagtcgg tctcgcgcga cggcaccggg cagccctcgc gcaccaggta gacctggccg
     2821 gtcgacacga acacgtaatg gccgacccgg ccgccgagca cgcgcacgct gcggaccgcg
     2881 tcgtcggcgg tgaaccctgc gaagtcgacc gcgcggtcga acgtccgtcc ggccagcgcg
     2941 cgatcgaacg catcgctcga gcggtcgacg tggatgcgct cgacgcgatc gccgaacgga
     3001 tccgccaggc gcccgcggtt gaccagcgtc acgcggtgtc cgccgcacag cagccgccag
     3061 accaggccgc ggcccaggaa gcgattgccg ccgatcacga gcacgttcat cgccggcgac
     3121 tctactcgcc cgcgccgccc gcacgctatc gagaccgcgc cgtcgaccgc tggctccggc
     3181 aaggcgccgt cagcaccgat agcgctgctg ccactctgtg atacaatcac cgaacgcacc
     3241 gacgacgtcg gtcgcaacac cggagcgttc gcgtgggaga cgtcggcaag cagaaccgca
     3301 ccgtaacgag cgatccaccc gtggcctcga gcgccgcaag cgaggggcat cgcacaccgg
     3361 gcacgccggg caaggccacg ctgaccgagg cgctgcccgt gatcgcagcg ccggtccagc
     3421 gcaaggccga cccggtctcg gagcggttcg acgacacagg agtgcaccag gcggcggccg
     3481 cgggtgtcgc gggcggcggc gggcagttgc cccacgtcgc cgagaccatc cgggtctacg
     3541 tcagcaacaa ggacacgccg cgattcgccg agtaccagaa gatcgtgccg caccgcttcg
     3601 agatcctgaa gaaggagtac gacaagtgat ccgcgcggcc gtcgcgtgcc tcgcgttcgg
     3661 cctggccggc tgcgcgtccg gtgcgcacca ggagccgccg agcaatgcgg ccacgaccgg
     3721 gagcccgggc cctggcccgg caggaagcga gaacaagatg actggagccg tatcgcccgc
     3781 gggagacaag caccccgggc agatcgtgta cgaggaccgg gtcgagaacc ggacctggac
     3841 ccgcaacgcc gccgaggtct cgcaggcgat ggcgtgggtc aaggtccacg atcagtggaa
     3901 gccggtggtg cggatcgaga tcaccggcgg cggcaacacc cgcgagatga cgtcgttcgg
     3961 ccccggccac gagttcctcg agaccaccac cgcgcagctg tcgggcgctc cgcatggcga
     4021 agcccttccg accccggtgc cgaccccgac tccgatcccc acctcgaagt agggtggccg
     4081 accggcgtct acttcgccgg ttcgagcttc gccgcgcaga acctggcgat cgccgcgagg
     4141 tgctggacgc ggttggcgtg cccgccgtgc gtcaccatca gctcgttcgc gatcccggcc
     4201 tcggtcaacg tcttgctgaa cgccgcggtc ggtgggtaga ggtcgaactc gtcggaatcg
     4261 ccgactgcca ggtagatgtg accgctgagc ggctcgagcg cggcgacgcg cgcggggtcg
     4321 cgcacccagg tggtcgggct attggcgatc cagcgcgcca gcacgctgtc gatcacatgg
     4381 cccgaagcat cgaacggcca gtcgtagccc gacggcgacg gcgaccagtc cgccgcgtag
     4441 ctgatgaact ggcccgcacc cccgaggccg tgctcgacgc gcgcccagcc gggcagccac
     4501 ggccggacat ccccggtccc gaaccacacc ggcaggtcga gcccgtcggg ggacgacgcg
     4561 ccgatcaccc cgatcagctc ggggtggcgc aggccgtacg agaccgcgtt gaatccgccg
     4621 gtcgaatggc cgaccacggc gcgcgcctcg cggcgcggca gcgcgcggta gtgtgcatcg
     4681 atatacggga tcagatcttt cgtgaagaac gtatcccatt tcccactggt cggactatcg
     4741 accaggtagg tcgagccggt cctggtgctg gtatcgaccc cgaccacgat cgtgtcctgg
     4801 agatcgagga gatagcctgg gaccgcggcg gcatcggtgg atagtagacc cggcagtgca
     4861 tagatcaccg ggtagtggcg ctcgggatgc tcggcgtagc ccctgggcac gcgcacgcac
     4921 gcgcgccgcg acgtcgggtt gccgaccgtg cccgcgacct ccggcgcctc gatgcgctcg
     4981 agcgtgagcc ggtccccctg gcaggcctcg ggccgctccc gcgacggacg actcgcgagg
     5041 gcgatcaccg gggccgctgc accggccggc ccgttgtcga acaccgcggt ggcgccgacc
     5101 agggtgccct tcccctcgcc ctggatcgcc ggcaggccga cgtgactgac gtcgagcgcc
     5161 gcgatcagcg cgatccgacc gcgaccggcg ggcaccgtga acgccgcgtt ttgcgccgcg
     5221 tccacgtcgg gggcgaccac gccgcgcgtg aggaggtcgc gcacgagcac gatgggcggt
     5281 cgaccctggt cgaacgccgt tttttccgcc tcggtcaacc agctgacaaa cagcgctccc
     5341 cgggtgatcg ccggcccggc ggccggatcc accgcaacat ggcccgtcac ggtggcgacc
     5401 gacggtgcca ccggcggcgg cgcggaggtc gtgtgacacg cgcagaacag cagtgcgacg
     5461 aggccgcggc gattcatggc cagggagtat cacgactcca cggccttgtg gagcagccgg
     5521 cgagctcgat ccacgccgga ctcgcactcc ggcaggtctt cgagatccgg gtccacgatg
     5581 ctccacggta tcgcgtcgag gatcggatgg tccttgccac cgtcgggatc gtagggtcgc
     5641 acgcgatagc tcgcgctggt ctgtgacgga ggcggagact gccaggcatc gatacccgtg
     5701 catccgtcca ggacctccat gccgagatgg cgtgcccaca gccggatccg caggtccttc
     5761 gcaaacccat ggggtggaca taccgaggtc gtcctgtcac agatgaacag accgacttcg
     5821 ctatcggatg acagtccgcg gttgttgacg ttcgccgagc cgacataggc aagctcgtcg
     5881 tcgaacaccc aggtcttcgc gtggatatac gtgttctcct gcgcatcggg cgggcgcacg
     5941 ggatcgacga ggtaatagac atgtagcttg tcgccgagcc ccttggagcg agcgcacgcg
     6001 atgaactcct tgcgacgccg ccacacctgc gggaggtcgc tgatggcact gccggtgatc
     6061 agggcgagca catacttcag gttctttgcg gcacggccca gcgcctcggc gacggtcagg
     6121 ctcgtcatgt actgatcttc gaagtagatg aacccgcgag cgctcgcgat ggcgccgagg
     6181 agaccgtcgc ggataccgcg ctcgaccttg caagcgcggc ggtcgagctt cttgagatcc
     6241 tcggggctga cccggttgaa cgttgtcgcg acacgcacga tctggcccgg ggctccgggg
     6301 acatcgggtc cgtgcgcgat cgtgcggccg aggacagggc cgaccgtctg atcgtagtgg
     6361 gcacccgacg ggtgcgagtc ccagcgttgc aggaagacct ggaccagatc cacggccgcg
     6421 gggccgcgca cctcgcagtg gacgtcgtgg agcggctcgc ccctgccatg ccggacgacc
     6481 gccacgcggt cggggttgag atcgacaccg ccgcacagcg cgtagagccc ttcgctgccg
     6541 cgcacgcaga gcaccttctg gtggtggcag ccagaccgtc gcggcgtcag cccgtcatcg
     6601 atcgcagcgg ctcccggcaa ggagttgatc cactcgacga tcctcttgtg gctgcgcgga
     6661 agatcggcga tctcggcact ccggttctgc gtgggatccg ccgggagatc gttcaggatc
     6721 ctcgcgtagt actcctcggg gtcgatgccc tgccgtacga tggcggcgag ctcgcccggt
     6781 gagtcgtgtg gggcaaacag cttcgacggg ttcggccaca gcatcgcgcg gatctgcacg
     6841 cccgcctttg ccttctcggg caggatctcg tgcaacgtgg acgatgggtc cggctcgtcg
     6901 ccggaccata cgagcggcag atcgggatcg agccaccacg cgagcagata gatgaagtcg
     6961 ccggatccgt gcgtcgtctg gattgcgcga tggatggctg cgagcgcagt ccggccgtcg
     7021 atgaacgact gcacggtgct gccgtgggtg aagtcgcggc cgagcgcctg cgcccacttg
     7081 agttccgctg gaagctgtgg catcccgttc ccgccgactc aagactgctg cctcgccacg
     7141 actacctttc ttacagccgc ctccactgcc tccgtttctg ggctgcatgg aagatttgat
     7201 gtccatggct aactaccaca gatctatcgg gagtcgagtg gtaaagttgg accggcgctc
     7261 agtccggggc gctgggcttg cgggtggcga ggatgcgctc gagcttggcg agctcggcgg
     7321 gcttgatgac gtgctcgtcg aagccggcgg cgcgggcggc ggagatgtcg ctcggggcgc
     7381 cgtagccggt cagggcgacg agcatgacgt cgtcgaggcc ggggcggtcg cggatgcgct
     7441 gggcgacctc gtagccgttc atggcgggca ggccgatgtc gatgaaggcg acgtcgggct
     7501 tctcgcgggt gatgacgtcg atcgcgctcg ggccgtcctc gacgtcgatc acgaggtggt
     7561 cgcgggactc gaggagcatg cgcagcatct cgcgcgagtc ggcctggtcg tcgaccagga
     7621 cgatgcggca ccggccgctg cggccgtcgc ccgagcgatc ggagccgagg tggaggacgg
     7681 cgcgcggcga cagcgggagg ctgaccttga agtcgctgcc ggtgtcgggg ccttcgctgt
     7741 ggacctcgat ggtgccgccg tggaggtcga cgatgttctt ggcgagcgac aggccgacgc
     7801 cgaggccgcc gcgcgagcgg tcgaggcgct gctcggactg gacgaacagg tcgaagatct
     7861 tgctctggag ctcggactcg atgccgacgc cgtggtcgat gacgcgcagc acgccgcggt
     7921 cgccctcgac ggtcacgacc aggcggacgg tgctgccgcg cggggagtag ttggcggcgt
     7981 tggacaagag gttgacgacg acctggacca ggcggctggc gtcgccgcgc atcggcatgg
     8041 cctggcgggg cagcgagtgc tcgagggtga cgccgcgctc ggcgaacagc ggggcggtcg
     8101 actcgatcgc ggcctcgatc gggccgcgca ggtcgacgtc gtcgaggatc agctggaact
     8161 tgccgcgggt gatgcgggag acgt
//
LOCUS       DASVOR010001381         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393498,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001381 DASVOR010000000
VERSION     DASVOR010001381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393498"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..966
                     /locus_tag="VF516_26285"
     CDS             325..966
                     /locus_tag="VF516_26285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX8111276.1"
                     /translation="MTPRLYPSWSNLVYRLALGLLLAGAASLVIVPMLYVRTPYNTSQ
                     FQPWDQPVEFDHRHHVRDDGIDCLYCHPGAERSASAGVPPTAVCMGCHDEIWQQSPML
                     EVVRRSYFSGRPIPWKRVHQLPGFVYFDHSVHIAHGFVCDRCHGDVSRMPRVTQVAPL
                     TMNWCLDCHRDPEAGGSERITDNAWAASMRMFFKRVANDRRVTLLTTCTTCHR"
     gene            963..>1834
                     /locus_tag="VF516_26290"
     CDS             963..>1834
                     /locus_tag="VF516_26290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111277.1"
                     /translation="MTRSDRSLPLAAVDPDPEFPPGADQPPELDRRRMIQLLAAGAAL
                     AGLGPVAGCMKKPSERIMPRVDQAPELTPGVALDYATATVIDGYATGVVVTTHEARPT
                     KVEGNPDHPASLGATSVFDQASVLELYDPDRPTGALDRGTPAELATLLRQIAGREPIP
                     GLWFLLRPQSSPLIEDAIARIRARHPAARFAFDTPVGRRAVFEGARLVFGRALEPQYR
                     FDIADAVVALDADFLAAMPNSVRWSRDFAARRRLASPGADMSRLYVAEPRPTPTGSLA
                     DHRLAVRGSEVRAIA"
BASE COUNT          236 a          704 c          641 g          253 t
ORIGIN      
        1 cgacggcctc gtcgacggcg ccgccggtca ccatcaggct ggcgctggtc agcacgccgg
       61 cgcgcagcgc gagcgccacc gcagcgttga cctcgcggga ccgtccgaag tcgtccgcgg
      121 tcacgatcaa cgcggggggc atgtgcgatc cggaaagcaa gcggtaggcc gcctgcgacg
      181 atcgggcggc tgagccgccg tcggccgcgg tcggtgcgcc gtcgcgcgtg ccgccgggcc
      241 acatcgtgat gtcggcgcat tgcggatccg ccgggaagcg ccgcgagcga tggactgcgc
      301 gttgcagtgc gtcggccgcg actcgtgacg ccgcggctgt atccatcctg gtccaacctg
      361 gtctaccggc tcgcgctggg gctgctgctc gcgggcgcgg cgagcctggt gatcgtgccg
      421 atgctgtacg tgcgcacgcc gtacaacacg tcgcagttcc agccgtggga tcagccggtc
      481 gagttcgatc accgccacca cgtccgcgat gacggcatcg actgcctgta ctgccacccg
      541 ggggcggagc gctcggccag cgccggcgtg ccgccgaccg cggtgtgcat ggggtgccac
      601 gacgagatct ggcagcagag cccgatgctc gaggtcgtgc ggcgcagcta cttctccggc
      661 cggccgatcc cgtggaagcg ggtgcatcag ctgccgggct tcgtctactt cgaccactcg
      721 gtgcacatcg cgcacggctt cgtgtgcgat cgctgccacg gcgacgtcag ccgcatgccg
      781 cgcgtcaccc aggtcgcgcc gctgaccatg aactggtgcc tcgactgcca ccgcgacccc
      841 gaggccgggg ggagcgagcg catcaccgac aacgcctggg cggcctcgat gcggatgttc
      901 ttcaagcggg tcgccaacga ccgccgcgtc accctgctca ccacctgcac cacgtgccac
      961 cgatgacccg atccgatcgc tcgctccccc tcgccgcggt cgaccccgac ccggagttcc
     1021 cgccgggcgc cgaccagccg cccgagctcg accgccgccg gatgatccag ctgctcgccg
     1081 ccggcgcggc gctcgccggc ctcggcccgg tcgccggctg catgaagaag ccgagcgagc
     1141 ggatcatgcc gcgcgtcgat caggcgccgg agctcacgcc gggcgtcgcg ctcgactacg
     1201 cgacggcgac ggtgatcgat ggctacgcga ccggggtcgt ggtcacgacc cacgaggccc
     1261 ggccgaccaa ggtcgagggc aaccccgatc acccggcgag cctcggcgcg accagcgtgt
     1321 tcgaccaggc ctcggtgctc gagctctacg atccggaccg cccgaccggc gcgctcgatc
     1381 gcggcacgcc ggccgagctc gccacgctgc tccgccagat cgccggccgc gagccgatac
     1441 cggggctgtg gttcctgctc cgtcctcaga gctcgccgct gatcgaggac gcgatcgcgc
     1501 ggatccgcgc ccgccatccc gccgcacggt tcgcgttcga cacgccggtc ggccgccgcg
     1561 cggtgttcga gggcgcgcgc ctggtgttcg gccgcgcgct ggagccccag taccggttcg
     1621 acatcgccga cgcggtcgtg gcgctcgacg ccgacttcct ggccgcgatg cccaactccg
     1681 tgcgctggtc gcgcgacttc gccgcccgcc gccggctggc gtcgccggga gccgacatgt
     1741 cgcggctgta cgtcgccgag ccgcgcccga cgccgaccgg cagcctcgcc gatcaccgcc
     1801 tggcggtgcg cggatccgag gtccgcgcga tcgc
//
LOCUS       DASVOR010001382         3159 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_422614,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001382 DASVOR010000000
VERSION     DASVOR010001382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422614"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VF516_26295"
     CDS             <1..465
                     /locus_tag="VF516_26295"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8111278.1"
                     /translation="LVAAALGVGSGVTSFALVSLARQPPATTSTGVSPTRDDTASARE
                     RSHSAAAGASAAPAAAPPIDAGAAGTPDPAAIAVRSAHPAPQPTGSLHVVGVPILTVT
                     VDGKPYGDTPLTIPLNAGTHRVRLQNVENNSDELLSITIVDHQTFTIDRMPQ"
     gene            490..1455
                     /locus_tag="VF516_26300"
     CDS             490..1455
                     /locus_tag="VF516_26300"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8111279.1"
                     /translation="MRRASLPTGTLAIAVLLALAGPARAGRNIGVVVTGESWMQPQLA
                     AQIATWLNEHGHTPTASTLDPETQAALNDCFVMHDERCARGVVEKAVRAPSVFYARID
                     THSNGSDVPDLTVTAYLFDRGHDAIAEKTTCQRCNDQSLRTAADAILQKLVGGGELGH
                     LGLKSAPPGARITIDGAAIGITPLDWDLPPGKHTIQMDKPGRRSGVRDHVVVSNKTDL
                     VVMTLPPDDRADQPSRVVPLALMIGGGAAVVAGAGLIAFSPGPDPHQRYYYRTWPPGI
                     AVTAGGAVLAGVGAYLRWFRSPAATSAPVASFTGDAAYVGWAGWF"
     gene            1506..2921
                     /locus_tag="VF516_26305"
     CDS             1506..2921
                     /locus_tag="VF516_26305"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8111280.1"
                     /translation="MIRLLLLVVPLSALAGCKTENKAFCDNMNNAGLQGCPGDAMNGG
                     GCGSDNDCKMMGFPACEKTINQGTCEPCTDSNIGVCTGTTPRCESNACVACADDVQDC
                     KGGVCLATGDCADTGHIIHASSSKTMMTTNCGDTAANACSLNSALAIVTTTKNVIKLD
                     DPGPFESDTGFVVNTNVTLDARGAILRRTGGGSNAVVTVNDNKNLTILGGTIESSQGD
                     GLKCGNNSMLAVYDSTITMNNNTGINGNGCTISVTHATISNNSKAPGAALFPGVQVAG
                     GAITISRSQILSNRGGGITVNNNATFTIVGNMFLSNGDSSGVVGGLQATTTVPGNRFD
                     FNTLSGNTTTAAVAAGVQCTAPAMSMTAPLTVQNNVIWDNNGPSGTQVSGTCLHAYSD
                     IGTTPVPGALTPPNANDFSQNPLFVGSSDLTVMSTSPIRGKANPNSDLGGIASMDILG
                     VPRTLRPGSGPDLGAYVVPDK"
BASE COUNT          580 a         1135 c          948 g          496 t
ORIGIN      
        1 ctcgtcgccg ccgcgctggg ggtcggaagc ggcgtcacct cgttcgcgct ggtctccctc
       61 gcgcggcaac ctccggcaac cacctcgacc ggcgtgtcac cgacccgcga cgacaccgcc
      121 tccgcccgcg agcgatcgca cagcgccgcg gccggtgctt cggccgctcc cgcggccgcg
      181 ccgccgatcg atgcgggcgc cgcgggcacc ccggatccgg ccgcgatcgc cgtacgatcc
      241 gcgcatcccg ctccgcaacc gaccggttcg cttcacgtcg tgggcgtgcc gattctcacg
      301 gtcacggtcg acggcaaacc ctacggtgac acaccgctca cgatcccgct gaacgcggga
      361 acccatcgcg tccggctcca gaacgtggaa aacaacagcg acgagctcct gtccatcacc
      421 atcgtcgacc accagacctt caccatcgac cggatgcccc aatgatcccc catccacgga
      481 cgtgctgaga tgcgccgcgc atccctcccc actggcaccc tcgcgatcgc cgtcctgctc
      541 gccctggccg gcccggcccg cgccggccgc aatatcggcg tcgtggtgac cggcgagagc
      601 tggatgcagc cccagctcgc ggcccagatc gcgacctggc tcaacgagca cggccatacc
      661 cccacggcgt cgaccctgga ccccgagacc caggcggccc tcaacgactg cttcgtcatg
      721 cacgacgagc gctgcgcgcg cggcgtggtc gagaaggcgg tccgcgcgcc cagcgtcttc
      781 tatgcacgga tcgacaccca cagcaacggc agcgacgttc ccgatctgac cgtgaccgcg
      841 tacctcttcg atcgcggtca cgacgcgatc gccgagaaga ccacctgcca gcgctgcaac
      901 gatcagtcgc tgcgcaccgc cgccgacgcg atcctccaga agctggtcgg cggcggcgag
      961 ctcggccacc tcggcctcaa gtcggccccg ccgggcgccc ggatcaccat cgacggcgcc
     1021 gcgatcggca tcaccccgct cgactgggac ctcccgccgg gcaagcacac gatccagatg
     1081 gacaagccgg gccgcaggtc cggcgtgcgc gaccacgtcg tcgtcagcaa caagaccgat
     1141 ctggtggtca tgacgcttcc gcccgacgac cgcgccgatc agccctcgcg ggtggtcccg
     1201 ctggccctga tgatcggcgg cggcgccgcc gtcgtcgccg gcgccggcct gatcgccttc
     1261 agccccggcc cggatcccca ccagcgctat tactaccgca cctggccgcc cggcatcgcc
     1321 gtcaccgccg gcggcgccgt cctcgccggc gtcggcgcct acctgcggtg gttccggtct
     1381 cccgcggcca cctcggcgcc ggtcgcgtcg ttcacggggg atgccgcgta cgtcggctgg
     1441 gccggctggt tctgaccgtg gcgtcgatca cagcgccatc gtttcgttct ctggaggatg
     1501 ctcccatgat tcgcttactg ctcctggtgg tcccgctctc cgcgctggcc ggctgcaaga
     1561 ccgagaacaa ggccttctgc gacaacatga acaacgccgg gctgcagggt tgccccggcg
     1621 acgcgatgaa cggcggcgga tgcggcagcg ataatgactg caagatgatg ggcttccccg
     1681 cgtgcgagaa gacgatcaac cagggcacct gcgagccgtg cacggacagc aacatcggcg
     1741 tgtgcacggg caccacgccg cgctgcgaga gcaacgcctg tgtcgcgtgc gccgacgatg
     1801 tccaggactg caaaggcggc gtgtgcctgg ccaccggcga ctgcgcggac accggccaca
     1861 tcatccacgc atcgtctagc aaaaccatga tgaccaccaa ctgcggcgat acggcggcaa
     1921 acgcctgctc gctgaacagt gcactcgcga tcgtcaccac gaccaagaac gtgatcaagc
     1981 tcgatgaccc ggggccgttt gaatccgata cgggcttcgt tgtgaacacg aacgtaacac
     2041 tcgacgcacg gggcgctatc ttgcggcgca ccggcggcgg cagcaacgcc gtcgtgaccg
     2101 tcaacgacaa caagaacctg acgattctcg gtggtaccat cgagagttcg caaggagacg
     2161 gcctcaaatg cggcaacaac tcgatgctcg ccgtctatga ttccacgatc acaatgaaca
     2221 acaacacagg aatcaacggg aatggctgca cgatctcggt cacgcatgcc acgatatcga
     2281 ataacagcaa ggcgccgggc gcggccctat ttccgggagt ccaggttgcg ggcggagcca
     2341 tcacgatttc acgctcccag atcctatcta atcgcggcgg cggtattacg gttaacaaca
     2401 atgcgacatt cacaattgtt ggaaacatgt tcttgagcaa cggggacagc agtggagtcg
     2461 taggtggact acaagcaaca accaccgtcc ccggaaaccg gttcgatttc aatacattgt
     2521 cgggaaatac caccacggcc gccgttgccg ctggagtcca atgcactgcg ccggcaatgt
     2581 caatgacagc tccgctcacg gtccagaaca atgttatctg ggacaacaac ggcccatctg
     2641 ggacccaggt cagcggaact tgcctgcatg cttactcgga tatcgggaca acacctgttc
     2701 ccggcgcctt gacgccaccc aacgccaacg acttcagcca gaacccactg tttgtcggct
     2761 cgagcgacct gaccgtgatg tcgacgagcc cgattcgcgg caaggcaaat cccaactcgg
     2821 atctcggtgg gatcgcctcg atggacatcc ttggtgttcc gaggacgtta cgtcctggct
     2881 cgggcccaga cctgggtgct tatgtcgtcc cggacaagtg agatcgatgg ccatggcgag
     2941 accacgttgc cgaggcgtac tcggttcggc gcggcacgac gcgagtcgct acgacaggcc
     3001 acgataggct aacatccccc gcatgaaggc gtgcctggtc gtgtccgtcg ccatgatggg
     3061 atgcgggttc ccgcgtccgc ctgacgtgaa ccccgatgct ccaagcgccg gcgatgcgcc
     3121 tgccgctccg gtgtgcttcg gcaagatcgt gccgatctg
//
LOCUS       DASVOR010001383         3760 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_540230,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001383 DASVOR010000000
VERSION     DASVOR010001383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540230"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..910
                     /locus_tag="VF516_26310"
     CDS             <1..910
                     /locus_tag="VF516_26310"
                     /inference="COORDINATES: protein motif:HMM:NF017518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zonular occludens toxin domain-containing
                     protein"
                     /protein_id="HEX8111281.1"
                     /translation="PHGRPLRLPVIPALADATTAERDQLARFAAAGLAAMMGYGTGTT
                     HRHKQSVLQCAIQLHADAREVTIDVLHDTINRPDPELLTRVGPLQRFFAPLSEDLQTL
                     NIQRGSLLAGNGEPLDAAALLPPAGERPRLSILSTAALTEIPVLQFWISRLLVELARL
                     ARKRPTKHLQAAVFFDEADAYIPANASPPTKEPMFELLRRARSEGVGILLATQNPGDL
                     DYKARDLIGTWLVGKVAQDRAIEKMRHLLSSYPNVGSRLATQPTGHFFVLGDGVREIK
                     ADRSMMSTEQLADAELAELARAGRPA"
     gene            1189..3306
                     /locus_tag="VF516_26315"
     CDS             1189..3306
                     /locus_tag="VF516_26315"
                     /inference="COORDINATES: protein motif:HMM:TIGR02148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX8111282.1"
                     /translation="MRKIVLATALCLFACGGGSNGNTPDGGSPGGPGCGDGMKQAGEG
                     CDDGNKVSGDGCSASCQEETGFKCITAGAPCVKIVACGDGMIQSPEVCDDGNTRPGDG
                     CDGTCRPEPGFTCATAGQPCVSNSVCGDGIKGGHEQCDDHNTASGDGCSATCTVEDGW
                     ACPDLGGKCAPKCGDGQIKGNEQCDLGPDNGQNKGCAADCTIQTGWVCGFNSSNVYGC
                     HQTVCGDSVKEGSEQCDDGNLIPFDGCSPTCAIEPVCAGGVCSATCGDGLMFPGEECD
                     DGNRVDGDGCDHNCKLEPSSGFNCPPKDLPPPGELDIPILYHDMRYNNTPSGIPDFEN
                     FNPGVVLFGLVKDTLGPDNKPVWSDDFGFLDAAKTKKNTDPNKQALSGQADYDCWWRA
                     NCNSAANTFAKNVFLNTAGKPTTLTLTKVNPQLPDDNVYQLDNQLFFPIDGLGWNASG
                     SGLPAQLSNGLDGKSHNFSFTSELHYPFTYLAGTNPTFDFTGDDDVWVFINGKLAVDL
                     GGVHGASNGSVTLDATTAAKFGLTDKGMYTIDMFQAERHTSASTYKLTLSGFVHTVTT
                     CTPICGDGKVVGNEVCDDGPNNGKAGFCNANCTAHLAKCGDGIIQPPEQCDNGVNNNA
                     YGTCNTDCTRAAFCGDGTKNGPEQCDNGTANNVGLDQYGPNLCTVACTVAPYCGDGIV
                     ETAFGEKCDGGPGCFDCQFAIIQ"
     gene            complement(3319..>3760)
                     /locus_tag="VF516_26320"
     CDS             complement(3319..>3760)
                     /locus_tag="VF516_26320"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8111283.1"
                     /translation="DVTRQVADSVAAGARLLCGGKRIGTRGNFYAPTALADLPAAAPA
                     YREEVFGPVAGVFKVQDAGDAIALANATPFGLGSSVWTTDLAEQRRFIDELDAGMTFV
                     NAMVASDPRLPFGGVKRSGHGRELSAVGIREFVNLKTVYVAAAR"
BASE COUNT          660 a         1299 c         1281 g          520 t
ORIGIN      
        1 cccgcacggc cggccgctgc ggctgccggt gatcccggcg ctcgccgacg cgacgaccgc
       61 cgagcgcgac cagctcgccc ggttcgccgc cgccgggctg gccgcgatga tggggtatgg
      121 caccggcacc acccaccgcc acaagcagtc ggtgctgcaa tgtgcgatcc agctccatgc
      181 cgacgcgcgc gaggtcacga tcgacgtgct gcacgacacg atcaaccgcc ccgaccccga
      241 gctgttgacc cgggtcgggc cgctccagcg gttcttcgcg ccgctgtccg aggacctcca
      301 gaccctgaac atccagcgcg gcagcctcct ggcgggcaac ggcgagccgc tcgacgccgc
      361 cgcgctcctg ccgccggcgg gggagcgccc ccggctgtcg atcctcagca ccgccgcgct
      421 caccgagatc ccggtgctgc agttctggat ctcgcggctg ctcgtcgagc tcgcccggct
      481 ggcgcgcaag cgcccgacca agcacctgca ggccgcggtg ttcttcgatg aggccgatgc
      541 ctacatcccg gcgaacgcca gcccgccgac caaggagccg atgttcgagc tgcttcgccg
      601 ggcgcggtcc gagggggtcg gcatcctgct cgccacgcag aaccccggtg acctcgacta
      661 caaggcccgc gatctgatcg gcacctggct ggtcggcaag gtcgcccagg accgggcgat
      721 cgagaagatg cggcacctgc tcagcagcta tcccaacgtc ggctcgcggc tggcgaccca
      781 gccgaccggc cacttcttcg tcctgggcga cggcgtccgc gagatcaagg ccgaccgctc
      841 gatgatgagc accgagcagc tcgccgacgc cgagctcgcc gagctcgccc gcgccggtcg
      901 ccccgcgtga cgtctgtggc gcacgcgctc gccggcgcaa gttttcgctc cgggtgaatt
      961 acgacagtcc agcggttcgc gttgggcgat ctggtgaccg ggaacggttg tatctgtcgc
     1021 cgactgtcgg tgcgctggat ccagggtgag cacggacctg tacacggagc gcgcacgtcc
     1081 atgtcgtcgc actccggcct accgatggag tgtggcaatc cgacacgcgg aaaagaccag
     1141 cagcccgagg cattgagtct tttctcgagg agcgtagtct gatccgtgat gcgcaagatc
     1201 gtactggcga cggcactgtg tctcttcgca tgtggtggtg gatcgaacgg gaatacgccc
     1261 gacggcggct cgccgggcgg cccgggctgc ggcgatggaa tgaagcaagc cggagagggc
     1321 tgcgacgacg gcaacaaggt ctccggcgac ggctgcagcg cctcctgcca ggaggagacc
     1381 ggcttcaagt gcatcaccgc gggcgccccg tgcgtgaaga tcgtcgcctg cggcgatggc
     1441 atgatccagt cgccggaggt ctgcgacgac ggcaacacca ggcccggcga cggctgcgac
     1501 ggcacctgcc gccccgagcc gggcttcacc tgcgcgacgg cgggtcagcc gtgcgtgtcg
     1561 aactcggtct gcggcgacgg aatcaagggg ggccacgagc agtgcgacga ccacaacacc
     1621 gcgtccggcg acggctgctc ggcgacctgc acggtggaag atggatgggc gtgcccggac
     1681 ctgggcggga agtgcgcccc caagtgcggc gacggccaga tcaagggcaa cgagcaatgc
     1741 gacctcggtc cggacaacgg ccagaacaag ggctgcgccg cagactgcac gatccagacc
     1801 ggctgggtgt gtggcttcaa cagcagcaac gtctacggct gtcatcagac cgtctgcggc
     1861 gacagcgtga aggagggcag cgagcagtgc gacgacggta acctgatccc gttcgatggc
     1921 tgctcgccga cctgcgccat cgagccggtc tgtgccggcg gcgtctgcag cgcgacctgc
     1981 ggcgacggcc tgatgttccc gggggaggag tgcgacgacg gcaaccgggt ggacggcgac
     2041 ggctgcgacc acaactgcaa gctcgagccg agctcgggct tcaactgtcc gccgaaggac
     2101 ctgccgccgc ccggcgagct ggatatcccg atcctgtatc acgacatgcg ctacaacaac
     2161 acgccgagcg gtatccctga cttcgagaac ttcaatcccg gagtggtgct gttcggcctc
     2221 gtgaaggaca cgctgggtcc ggacaacaag ccggtgtgga gcgacgactt cggtttcctg
     2281 gacgcggcca agaccaagaa gaacacggac cccaacaagc aggccctgag cgggcaagcc
     2341 gactacgact gctggtggcg cgccaactgc aacagcgcgg ccaacacgtt cgccaagaac
     2401 gtgttcctca atacggcagg caagcccacg acgctgacgc tgacgaaggt gaacccgcag
     2461 ttgcccgacg acaacgtcta tcagctcgat aaccagctgt tcttcccgat cgacgggctg
     2521 ggctggaatg cgtcggggtc cgggctcccc gcgcagctca gcaacggcct cgacggcaag
     2581 tctcacaact tctcgttcac cagcgagctg cactacccgt tcacctacct ggcggggacg
     2641 aatccaacgt tcgacttcac cggcgacgac gacgtctggg tgttcatcaa cggcaagctc
     2701 gcggtcgatc tcggcggcgt ccacggcgcg agcaacggca gcgtcacgct cgacgccacg
     2761 accgccgcga agttcggcct gaccgacaag ggcatgtaca cgatcgacat gttccaggcc
     2821 gagcgccaca cctccgcgtc gacctacaag ctgacgctgt cggggttcgt ccataccgtg
     2881 accacgtgca ccccgatctg cggcgacggc aaggtcgtgg gcaacgaggt atgcgacgac
     2941 ggccccaaca acggcaaggc gggcttctgc aacgcgaact gcacggccca cctggcgaag
     3001 tgcggcgacg gcatcatcca gccgcccgag cagtgcgaca acggcgtgaa caacaacgcg
     3061 tacggcacct gcaacaccga ctgcacgcgg gcggcgttct gcggcgacgg caccaagaac
     3121 ggccccgagc agtgcgacaa cggcacggcg aacaacgtcg gcctcgacca gtacggcccg
     3181 aacctgtgca ccgtcgcctg cacggtcgcc ccgtactgcg gcgacggcat cgtcgagacc
     3241 gcgttcggcg agaagtgcga cggcggcccg ggttgcttcg actgccagtt cgcgatcatc
     3301 cagtgatcgc ggcggcggtc accgcgcggc ggcgacgtag acggtcttga ggttgacgaa
     3361 ctcgcggatg ccgacggccg acagctcgcg gccgtggccg gagcgcttga cgccgccgaa
     3421 cggcaggcgg gggtcggagg cgaccatggc gttgacgaac gtcatgccgg cgtcgagctc
     3481 gtcgatgaac cgccgctgct cggcaaggtc ggtggtccac accgaggagc cgaggccgaa
     3541 cggggtcgcg ttggcgagcg cgatcgcgtc gcccgcgtcc tggaccttga acacgccggc
     3601 gaccgggccg aacacctcct cgcggtaggc cggcgcggcg gcgggcaggt cggcgagcgc
     3661 ggtcggcgcg tagaagttgc cgcgcgtgcc gatccgcttg ccgccgcaca gcaggcgcgc
     3721 accggcggcg accgaatcgg cgacctggcg ggtgacatcg
//
LOCUS       DASVOR010001384         1861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_510905,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001384 DASVOR010000000
VERSION     DASVOR010001384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510905"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1861
                     /locus_tag="VF516_26325"
     CDS             <1..>1861
                     /locus_tag="VF516_26325"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8111284.1"
                     /translation="AFALPRAEVGQGITTAVAMTIADEMGMPLDKVHVTLADARPELV
                     WNQITAGSNSMHSLFTPVRVAAAIARERLLEAAARFLGQPLGNLSLVDGVVTSVTGQT
                     VAVGDLAGLAAVSETVAVQPTLKSPSQFKLIGTPQRRVDAHDIVTGAKVFAMDLDIPG
                     ALPTMLCRAPTINASAIAVRNLAVVKAFPGITDVVIIPHTAFVPGGVAVRGQTFGQCI
                     TAIRALDVAWGPGTVDGKSDATVLADLRANELPLTPPLLGHVLEDTFVFHFRPGDPLE
                     TNCAVADVRSDRAEVWAALKTPIWCKEQLALNLGLPLDRVVVHVAEAGGSFGRHLFPD
                     AAFEAAEVSRVLGKPVKLMWHRTDNFRQGRVHPMCTSHVRLTHLLGNVLTFDQRHTSV
                     ATDFSQGFGELLTAIADTLPGVNGLGFAQLIFSLTANVPYNFGVVTQLLHEIYAFNTF
                     NTSSVRNIYSPEVCTARELMVDRAARAMGKDPYSFRREFVRDARLHAVLDRVASVGNW
                     GRTMAAGTAQGIALHKEYKGAAACLVEIDCRSATVNRQIPDACTGPRVTKVVYAVDVG
                     LPINPLGLEAQMMGGIMDGIAQALSYSLHLDHGHFLEGSWDNAYYTRQWNTPPS"
BASE COUNT          279 a          696 c          598 g          288 t
ORIGIN      
        1 ggccttcgcg ctgccgcgcg ccgaggtcgg gcagggcatc acgaccgcgg tggcgatgac
       61 gatcgccgac gagatgggca tgccgctcga caaggtccac gtgacgctgg ccgatgcgcg
      121 gccggagctc gtgtggaacc agatcaccgc gggctcgaac tcgatgcact cgttgttcac
      181 gcccgtccgc gtcgccgcgg cgatcgcgcg cgagcgactg ctcgaggccg cggcgcggtt
      241 cctcggccag cccctcggca acctgagcct cgtcgacggt gtcgtcacct ccgtcaccgg
      301 gcagacggtg gcggtcggtg atctggccgg cctcgcggcg gtgtccgaga ccgtcgcggt
      361 ccagcccacg ctcaagtcgc cgtcgcagtt caagctgatc ggcacgccgc agcgccgcgt
      421 cgacgcacac gacatcgtca ccggcgcgaa ggtcttcgcg atggatctcg acatccccgg
      481 cgccttgccg acgatgctct gtcgcgcgcc gaccatcaac gccagcgcca tcgccgtccg
      541 caacctcgcc gtggtgaagg ccttccccgg catcaccgac gtggtcatca tcccgcacac
      601 ggccttcgtt ccgggcggcg tggcggtgcg cgggcagacc ttcggccagt gcatcaccgc
      661 catccgtgcg ctcgacgtcg cgtgggggcc gggaacggtc gacggcaagt ccgacgccac
      721 cgtgctcgcc gacctcaggg ccaacgagct gccgctcaca ccgccgctcc tcggccacgt
      781 cctcgaggat accttcgtct tccacttccg tcccggcgac ccgctcgaga ccaactgcgc
      841 cgtggccgac gtgcggagcg accgcgccga ggtgtgggcg gccctgaaga cgccgatctg
      901 gtgcaaggag cagctcgcgc tgaacctcgg cctgccgctc gaccgggtgg tcgtccacgt
      961 ggccgaggcc ggcggctcgt tcggccgtca cctgttcccc gatgccgcct tcgaggccgc
     1021 cgaggtgtcg cgcgtcctcg gcaagcccgt caagctgatg tggcaccgca ccgacaactt
     1081 ccgtcagggg cgcgtgcacc cgatgtgcac ctcgcacgtc cgcctgaccc acctgctcgg
     1141 caacgtcctc accttcgatc aacgccacac gagcgtcgcg accgacttct cgcagggctt
     1201 cggcgagctg ctcaccgcga tcgccgacac gctgccgggt gtgaacggcc tcggcttcgc
     1261 ccagctgatc ttttccctca ccgcgaacgt gccttacaac ttcggtgtcg tcacccagct
     1321 cctccacgag atctacgcgt tcaacacgtt caacacctcg tcggtgcgca acatctatag
     1381 ccccgaggtg tgcaccgcga gggagctcat ggtcgatcgc gccgcacgcg cgatgggcaa
     1441 ggacccctac agcttccggc gcgagtttgt tcgcgatgct cggttgcacg cggtcctcga
     1501 ccgggtcgcg agcgtgggca actggggtcg gaccatggcg gccggcaccg cccaggggat
     1561 cgcgctccac aaggagtaca agggcgccgc ggcgtgcctc gtcgagatcg actgccgctc
     1621 ggcgaccgtc aaccgccaga tccccgacgc ctgcaccggg ccgcgcgtga ccaaggtggt
     1681 ctacgccgtg gacgtcgggc tgccgatcaa ccccctcggt ctcgaggccc agatgatggg
     1741 cgggatcatg gacggcatcg cccaggcgct gagctacagc ctgcacctcg accacggcca
     1801 tttcctcgaa ggcagctggg acaacgccta ctacacgcgg cagtggaaca cgccgccgag
     1861 c
//
LOCUS       DASVOR010001385         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_422641,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001385 DASVOR010000000
VERSION     DASVOR010001385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422641"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VF516_26330"
     CDS             complement(<1..568)
                     /locus_tag="VF516_26330"
                     /inference="COORDINATES: protein motif:HMM:NF037004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111285.1"
                     /translation="MLRVQPALRPRAVPGLPAVFVVLSLVFAGCSAKAPASGESRPAT
                     PEVKAAPAAPTEPAPALDPRETTLSAAVVLLLEHEHLLRKPIDDELSRTAYDTYLDRL
                     DVGKMFLLRKDRDALVRYVDKVDDELRSGALDLAHDGERIFESRVEVVEKLVAELLSA
                     PRSAVDAGGRPGCGGAAPVAGLRQRDESD"
     gene            816..974
                     /locus_tag="VF516_26335"
     CDS             816..974
                     /locus_tag="VF516_26335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111286.1"
                     /translation="MAVVSIIRGIDRIGDGHPASGWIFLASGSAIVAGLVQALRASRS
                     AQRPQASR"
     gene            1039..1344
                     /locus_tag="VF516_26340"
     CDS             1039..1344
                     /locus_tag="VF516_26340"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8111287.1"
                     /translation="MTASPLVVHGTALSGHTHRVLNFLGLLDLPHRLVDAPAAVRRTP
                     EFLALNPLAQVPVLQDGDVVLADSNAILVYLARRYAAGSPEAVPGWTPMPVAPGPQA"
     gene            1356..>1999
                     /locus_tag="VF516_26345"
     CDS             1356..>1999
                     /locus_tag="VF516_26345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111288.1"
                     /translation="MPDDDRTHAPGPAAPTAGPFHPGELTAQQLAGGGSAGGAIRSFM
                     PDQHRRFFAQLPFIAVATTDDHGPVATLWTGRPGFVHSPDPQTLRIAIALDPADPASG
                     AFIAGAPFGLLGIELATRRRNRANGAITAVDPDGVTVSVRQSFGNCPKYIHPRDAVTA
                     PGSAAPAEWLHGVDRAAAAAIAAADTFFVATAARTGEPAGGVDISHRGGPPGFVR"
BASE COUNT          269 a          822 c          656 g          252 t
ORIGIN      
        1 catcagattc gtcacgttga cgcaggccag caacaggagc agcgccgccg cagccaggac
       61 gaccaccagc gtcgaccgca gatctcggcg cggacagcag ctccgccacc agcttctcga
      121 ccacctcgac ccgggactcg aagatccgct cgccgtcgtg cgcgaggtcg agcgcgccgg
      181 agcgcagctc gtcgtcgacc ttgtcgacgt agcgcacgag cgcgtcgcgg tccttgcgca
      241 agaggaacat cttgccgacg tcgagccggt cgaggtacgt gtcgtacgcg gtgcgcgaca
      301 gctcgtcgtc gatcggcttg cgcagcaggt gctcgtgctc gagcagcagc accaccgccg
      361 ccgacagcgt ggtctcgcgc gggtcgagcg cgggagccgg ctcggtcggc gcggcgggcg
      421 ccgccttgac ctccggcgtg gccggccgcg actcgcccga tgccggcgcc ttggccgagc
      481 atcccgcgaa caccagactc aggacgacaa agaccgccgg caggccgggc acggcccgcg
      541 ggcggagagc aggttggacg cgcagcacgc ctgtaatcta gcgtgcccgg gccagctcgg
      601 ccgcacgaga acgcgccaac caccccccgt gaacccggct ggaccacgcg ctgtcctggt
      661 gcgtcacacc accccgacgt gccgcacctg gctgcttgcc gccgcctcgc ggcatgccta
      721 cggtgtcgct tgcaactccc atgagcgcga tgatcgaatc ctccacgccg ccagcgcgga
      781 acgtccctcg gtggttatgg ccaggcatga cggcgatggc cgtcgtatcg atcatccggg
      841 gcatcgaccg gatcggggat ggccacccgg cgtcgggctg gatcttcctg gcgtccggga
      901 gcgcgatcgt ggcgggcctc gtgcaggcgc tccgggcgtc tcgctcggcg cagcgcccgc
      961 aggcgtcgcg atagacccgc ggtgcacggc ggggctgggc cacgcggccg gccgcgcggt
     1021 atcaacgcag gaaccgccat gaccgcctcg cccctcgtcg tccacggcac cgcgctgtcc
     1081 ggccacaccc accgggtcct caacttcctc ggcctgctcg acctgccgca ccggctggtc
     1141 gacgccccgg ccgccgtgcg ccggaccccc gagttcctcg cgctcaaccc gctcgcccag
     1201 gtcccggtgc tgcaggacgg cgacgtggtg ctcgccgaca gcaacgcgat cctggtctac
     1261 ctcgcccggc gctacgccgc cggatcgccg gaggccgtcc ccggctggac cccgatgccg
     1321 gtggcgccag gtccccaggc ctaggcaagg caaccatgcc cgacgacgac cgcacgcacg
     1381 cccctggccc cgcagcaccg accgcgggcc cgttccaccc cggcgagctc accgcccagc
     1441 agctcgccgg gggcggctcc gccggcggcg cgatccgctc gttcatgccc gaccagcacc
     1501 gccggttctt cgcccagctg ccgttcatcg ccgtcgccac caccgacgac cacgggccgg
     1561 tcgcgacgct gtggaccggc cgccccggct tcgtccactc gcccgacccg cagacgctgc
     1621 gcatcgcgat cgccctcgat ccggccgacc ccgcgagcgg cgcattcatc gccggcgctc
     1681 cgtttggcct gctcggcatc gagctcgcga cccggcggcg caaccgcgcc aacggcgcga
     1741 tcaccgcggt cgaccccgac ggcgtcaccg tctcggtccg ccagagcttc ggcaactgcc
     1801 cgaagtacat ccacccgcgc gacgccgtca ccgccccggg ctcggccgct cccgccgaat
     1861 ggctccacgg cgtcgaccgc gccgccgccg ccgcgatcgc cgccgccgac acgttcttcg
     1921 tcgcgaccgc cgcccgcacc ggcgagccgg ccggcggcgt cgacatctcg caccgcggcg
     1981 gcccgcccgg cttcgtccg
//
LOCUS       DASVOR010001386         1523 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_248194,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001386 DASVOR010000000
VERSION     DASVOR010001386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1523
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248194"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1523
                     /locus_tag="VF516_26350"
     CDS             <1..>1523
                     /locus_tag="VF516_26350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111289.1"
                     /translation="TVAGLAPPLTAPPSLPPARYQLGPEIARGGMGRVVDALDTRLGR
                     TVAFKEALSADRDALQRFEREIRITARLEHPSIVPVHDAGESGNGAPFYVMRKVSGRP
                     LEKLVASAETLGQRLALIPHIVDSAQAIAHAHERGIVHRDIKPSNILVGELGETVVID
                     WGLAKVIGEPEPDAHVARPLVDLSDTLKTRAGIVYGTPGFMAPEQLCGAPVDERCDVY
                     ALGATLYHLLARTPPHHAKTADEMMKAAVAAPPTPIRELVPGVPLDLSTIVDKALAHD
                     PQIRYQNARTLAEDLQRFLTGQLVASHHYTPREKLIRFVRKNRGVSAAVAALLVVGTG
                     LMIKIYVERNRAQRAEAIAEAHRAEAERRAEELTLTQARYNVDVNPTRAVLMVKPLAA
                     KYWREVRAIAAAAHAAGVAWSLPAAQETLSLEIGHDGTRAVSGGQDGVVRIHDLLRRT
                     TRPVLDLRAPAMARLGDGDRRIVAWHDRQLVIADAATGQRRDVTAQRPIADLEVVAT"
BASE COUNT          215 a          591 c          537 g          180 t
ORIGIN      
        1 gacggtggct ggcctggcgc cgccgctgac cgccccgccg tcgctgccgc cggcgcgcta
       61 ccagctcggc cccgagatcg cgcgcggcgg tatgggccgc gtggtcgacg cgctcgacac
      121 ccggctcggc cgcaccgtcg cgttcaagga ggcgctgtcc gccgaccgcg atgcgctgca
      181 gcggttcgaa cgcgagatcc ggatcaccgc gcggctggag catccgtcga tcgtcccggt
      241 gcacgacgcc ggcgagtccg gcaacggcgc gccgttctac gtgatgcgca aggtgtcggg
      301 ccggccgctc gagaagctgg tcgcgagcgc cgagaccctc ggccagcggc tcgcgctgat
      361 cccgcacatc gtcgacagcg cgcaggcgat cgcgcacgcc cacgagcgcg gcatcgtcca
      421 ccgcgacatc aagccgtcga acatcctggt cggcgagctc ggcgagaccg tggtgatcga
      481 ctggggcctc gccaaggtga tcggcgagcc cgagcccgac gctcacgtgg cccggccgct
      541 ggtcgacctg tcggacacgc tcaagacccg ggccgggatc gtgtacggca caccgggatt
      601 catggcgccc gagcagctgt gcggcgcgcc ggtcgacgag cgctgcgacg tctacgcgct
      661 gggcgcgacg ctgtaccacc tgctggcgcg cacgccgccg caccacgcca agacggccga
      721 cgagatgatg aaggccgcgg tcgccgcgcc gccgacgccg atccgcgagc tggtccccgg
      781 cgtgccgctc gacctgtcga cgatcgtcga caaggcgctc gcccacgacc cccagatccg
      841 ctaccagaac gcgcgcacgc tcgccgagga cctccagcgc ttcctcaccg gccagctcgt
      901 cgcgtcgcac cactacacgc cgcgggagaa gctgatccgg ttcgtccgca agaaccgcgg
      961 ggtgtcggcc gcggtggccg cgctgctcgt ggtcggcacc ggcttgatga tcaagatcta
     1021 tgtcgagcgc aaccgcgccc agcgcgccga ggccatcgcc gaggcccacc gcgccgaggc
     1081 cgagcgccgc gccgaggagc tgacgctcac ccaggcgcgc tacaacgtcg acgtcaaccc
     1141 gacccgggcg gtcctgatgg tcaagccgct cgccgccaag tactggcgcg aggtccgggc
     1201 gatcgcggcc gccgcccacg ccgcgggcgt cgcgtggagc ctgcccgcgg cccaggagac
     1261 cctgtcgctg gagatcggcc acgacggcac ccgcgcggtg tcgggcggcc aggacggcgt
     1321 ggtccggatc cacgacctgc tccgccgcac cacccggccg gtgctcgacc tccgcgcccc
     1381 ggcgatggcg cggctgggcg acggcgaccg gcggatcgtc gcctggcatg atcgccagct
     1441 ggtgatcgcc gacgcggcga ccggccagcg gcgcgacgtc accgcccagc ggccgatcgc
     1501 cgacctcgag gtcgtggcca cca
//
LOCUS       DASVOR010001387         6232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393534,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001387 DASVOR010000000
VERSION     DASVOR010001387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6232
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393534"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..999)
                     /locus_tag="VF516_26355"
     CDS             complement(289..999)
                     /locus_tag="VF516_26355"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC N-terminal beta barrel domain-containing
                     protein"
                     /protein_id="HEX8111290.1"
                     /translation="MTGRDVGRVAALWRYPVKSMAPEALERVEVEWHGIAGDRRWAFV
                     RADQERNGFPWLTIRELPELARYQPRLVDPARPDETAVRVCTPAGAELDVADPTLAAE
                     LGAGVRVMKLDRGAFDTLPLALISRQTLAGLGALVGRELAVQRFRPNLVIDGADGAAY
                     PEDAWVGATLQIGALRMRVDKRDQRCVVVNLDPVTGARDPAILNTIGRARQACLGVYG
                     SVVTPGAIGVGDPVMLDA"
     gene            1336..1782
                     /locus_tag="VF516_26360"
     CDS             1336..1782
                     /locus_tag="VF516_26360"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8111291.1"
                     /translation="MATDDQGQGDNQLAGAETDRPAFDMRQFVTRYFALALDAPDVSE
                     FTELFVPDGVLEDPVGTPPLRGREAIGQFLAAGRSLIEHSQYEIRDVLSCGHESAVRW
                     SAVVTTRRGQRVAVEGIGIFSFDDQRKLRSVREFYDVRTLQQIFSA"
     gene            complement(1788..2129)
                     /locus_tag="VF516_26365"
     CDS             complement(1788..2129)
                     /locus_tag="VF516_26365"
                     /inference="COORDINATES: protein motif:HMM:NF021593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2325 domain-containing protein"
                     /protein_id="HEX8111292.1"
                     /translation="MVVPGRRGPGSVADDGMRGAVIVVGGSGGMSERYRAVVAERGLE
                     LRHFENRVPNGMRRIVGRVAAVFIMVGMVSHALRDQVEALVPDGAPVVYLRSASISAL
                     RSAVASLDAGS"
     gene            2443..3204
                     /locus_tag="VF516_26370"
     CDS             2443..3204
                     /locus_tag="VF516_26370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8111293.1"
                     /translation="MARTALVTGGNRGIGQQVARVLAGDGWDVLVGARDRQKGDAAAA
                     RLRKETGGRLKAIELDVTSDASIAAAAQKLRDGAIWIDTLVNNAGVYGAARGPDGVAR
                     TIETNFFGPLRVTLGLLPLLRDGATITNVTSGLGALDHLDSAHRRLLADPALTRDDLV
                     AHMREFVRTGGQGWGTDAYGVSKAALNALTRVLAHELAPRQIRVNATCPGWVRTDMGG
                     RGAPRSVEEGAASVLFGVLATETGGVFRDGRRIAP"
     gene            complement(3247..3786)
                     /locus_tag="VF516_26375"
     CDS             complement(3247..3786)
                     /locus_tag="VF516_26375"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8111294.1"
                     /translation="MRPRFSVFIAASLDGFIARADGNIDWLSIHALDQEPNGYYEFIR
                     TVDAIVIGRGTYETALGFGGWVYGNKRVIVLTHGARESRHGEEFHAGDPAGLLPRLAD
                     CKRVYVDGGKVISQFFAADLIDDVTVGILPIVLGGGIRLFPGGEGEHRLALERHHVYR
                     SGMVQLAYRVRRPEGAPAR"
     gene            complement(4963..>6232)
                     /locus_tag="VF516_26380"
     CDS             complement(4963..>6232)
                     /locus_tag="VF516_26380"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8111295.1"
                     /translation="KLEQVADDFEQTGELERAFDCFQILLKLGKESAQFENLAEGYVN
                     CIRVLRDDNLKFYVLQYYEDFIKLALERGELHAAATLYQEAAAFAARAGLPYDRHYQH
                     KSALTWMKCADKFVETGVPVQMVENALLAAASQHSAVGDYPAVRECFDKLAGLELPER
                     AKKRFATIAQRYRGLAAPPVELPGLPDYLKQQHAYADIWFVDLLEWEMDGDPHAVAAS
                     IVGDLRYPNGIRRRALVVLLTLADAQTRRAQAEPETLVHVAELLGELQSYAALSPLEK
                     LFASGDAVIRRAAVRALRYLYFKRSFVIVRKALTDPDAQVREAALVAIAGLHFPHAFN
                     PLARIYRETSDPRVRAAALQSIGKIQTVEAGEFLVMVLRQETGGLREAAYAALTQMDN
                     ADVLPILRQHHEIETNPAVRDTLGDLLRRH"
BASE COUNT         1006 a         2106 c         2190 g          930 t
ORIGIN      
        1 gccatcctcg tcgcccgccc ccgacgctgc gcgtacgatc agatggcgcg ccggcgtgat
       61 cgccagcgcc agccgggtcg gcccgcggtc gtcggcagac cgactcgcag acgagcgcgt
      121 acctcgcacg gccatcccac agctctagca cggaccgctg acacggctgc ggcgctgcat
      181 gcaccgcgtg cacgtccaag cccgggcacc gcctccgctc ccgccgcagc catgtgccct
      241 cccatgcgag gatctccgcc gctcggcccc ggacgtgcgg agcgcagctt acgcgtcgag
      301 catcaccgga tctcccaccc cgatcgcgcc cggcgtcacg accgatccgt agacgccgag
      361 acacgcctgc cgcgcgcggc cgatcgtgtt caggatcgcc ggatcccgcg cgccggtgac
      421 cgggtcgagg ttgaccacga cgcagcgctg atcgcgcttg tcgacccgca tccgcagcgc
      481 gccgatctgg agcgtcgcgc cgacccaggc gtcctcggga tacgccgcgc cgtcggcgcc
      541 atcgatcacg aggttgggcc ggaaccgctg gacggcgagc tcgcggccga cgagcgcgcc
      601 gaggccggcg agcgtctggc gggagatcag cgcgagcggc agggtgtcga acgcgccgcg
      661 gtcgagcttc atgacccgca cgcccgcgcc cagctcggca gcgagcgtcg gatcggcgac
      721 gtcgagctcg gcgccggccg gcgtgcagac cctcacggcc gtctcgtccg gacgcgccgg
      781 atcgacgagc cgcggctgat agcgcgcgag ctccggcaat tcgcggatcg tgagccacgg
      841 gaacccgttg cgctcctggt cggctcgcac gaaggcccat cggcgatcgc cggcgatgcc
      901 gtgccactcg acctcgacgc gctcgagcgc ctcgggtgcc atcgacttga ccgggtaccg
      961 ccacagcgcc gcgacccggc cgacgtcccg ccccgtcacg cgcggcctcc gcttcgagcc
     1021 cccaggagcg acccaggagc aacaataaac tgcaacgacc acatggtgcc tcctcgagag
     1081 aggcgccctt gcgtggagat tcggcgccga gcgtggcggg tggagcccgt tgcagcgcct
     1141 ctcggcgacg agtgcgcact gcggcgcacg tgatcatggt gaatcgctac cggatcaccg
     1201 tggcatgccg gcacgtggac gtcaaccaca gcttccgcga cgacgatcat ccgatgctcc
     1261 acgacccggc gaacctggat ctcatgtcgt gcgccgcgca atggatgcca tggcgattca
     1321 cggtatcgtt ccaccatggc caccgacgat cagggccagg gagacaacca gctcgccggt
     1381 gccgagacgg atcggccagc gttcgacatg cgccagttcg tgaccaggta tttcgcgttg
     1441 gccctcgatg cacccgacgt gagcgagttc acggagctgt tcgtgcccga cggggtcctg
     1501 gaagatccgg tggggacgcc accgcttcgc gggcgggagg ccattggcca gttccttgcc
     1561 gcgggccggt cgctgatcga gcatagccag tacgagatcc gcgacgttct gtcctgtggg
     1621 cacgagagcg ccgtgcgctg gtcggccgtc gtgacgacca ggcggggcca gcgcgtcgcc
     1681 gtcgaaggaa tcgggatctt ctccttcgac gaccagcgca agctccgttc cgtgcgagag
     1741 ttctacgacg tcaggacact ccagcagatc ttttccgctt gagatgttca ggagccggca
     1801 tcgagagaag cgaccgcgct ccgcagggcg gagatcgatg ccgatcgtaa gtagaccacc
     1861 ggagcgccgt cggggacgag cgcctccacc tggtcgcgca gcgcgtggga gaccatgccg
     1921 accatgatga agacggcagc gacccgaccg acgatgcgtc gcattccatt gggcacgcgg
     1981 ttctcgaagt ggcgcagctc cagcccgcgc tccgcgacga cggctcggta gcgctccgac
     2041 atgccgccgc taccaccgac gacgatcacc gcgcccctca tcccgtcgtc cgccacgctg
     2101 ccggggccgc gtcgccccgg cacgaccatg gacttgcttg ccatggtcag agcacgagca
     2161 agcgctgagc cagcgagcac cccgatgcga aaaccagcgg ttctgcggcc gccggcgtct
     2221 gatcgctgca aacttggcaa agcgagaccg gcgcagaggg tcgtacggac aaaatgtccg
     2281 gtccgccgtc gccggcgccg cgcgctggag accgcctgcc ctggtgctcg ccgcctgcaa
     2341 ccagagtctc gacgacgcgg tcgcgctgcg tggatcgcgg gttgctcgat cgcgccggcc
     2401 gtgcaagccg agcggagggc ggcgcggtaa agtccatccg ccatggccag aaccgcgctc
     2461 gtcaccggcg gaaatcgcgg catcggccag caggtcgcac gcgtgctcgc cggcgacggc
     2521 tgggacgtgc tcgtcggcgc gcgcgaccgg cagaagggcg acgccgcggc ggcgcggctt
     2581 cgcaaggaga ccggcgggcg gctcaaggcg atcgagctcg acgtgacgag tgacgcgtcg
     2641 atcgccgccg ccgcgcagaa gctccgcgac ggcgcgatct ggatcgacac gctcgtgaac
     2701 aacgccggcg tgtacggtgc cgcgcgcggc cccgatggcg tcgcgcgaac gatcgagacc
     2761 aacttcttcg ggcctctgcg cgtcacgctc gggctgctgc cgctgctgcg cgacggcgcc
     2821 acgatcacca acgtgacgag cgggctcggc gcgctcgacc acctcgacag cgcacaccgg
     2881 cggttgctcg ccgacccggc gctcacgcgc gacgatctcg tcgcgcacat gcgcgagttc
     2941 gtgcgcaccg gcggccaggg ctggggcacc gacgcctacg gcgtctcgaa ggccgcactc
     3001 aacgcgctga cgcgggtcct cgcgcacgag ctggcgccgc gccagatccg cgtgaacgcg
     3061 acatgcccgg gctgggtgcg caccgacatg ggcggccgcg gcgcgccgcg ctcggtcgag
     3121 gagggtgccg cgagcgtgct gttcggcgtc ctcgcgacgg agaccggcgg tgtgtttcgc
     3181 gacgggcgtc gaatcgcgcc ctgaggagtg ctcgaaccgc ggcgggacga ccgcggactg
     3241 cggatgtcag cgcgcgggcg ctccctccgg gcgccgcacg cgatacgcga gctgcaccat
     3301 gccgctgcgg tagacgtggt ggcgctcgag cgcgaggcgg tgctcgcctt cgccgcccgg
     3361 aaacagccgg atcccgccgc cgagcacgat gggcaggatg cccacggtca cgtcgtcgat
     3421 cagatcggcg gcgaagaact gcgagatgac cttcccgccg tcgacgtaga cccgcttgca
     3481 gtccgccagc cggggcagca ggcctgctgg atcgccggcg tggaactcct cgccgtgccg
     3541 cgactcccga gcaccgtgcg tcaggacgat cacgcgcttg ttgccgtaga cccagccgcc
     3601 gaagccgagc gcggtctcgt aggtgccgcg gccgatgacg atcgcgtcga cggtgcggat
     3661 gaactcgtag tagccgttgg gctcctggtc cagcgcatgg atgctcagcc agtcgatgtt
     3721 gccgtcggcc ctggcgatga acccgtcgag gctggcggcg atgaagaccg agaaccgagg
     3781 gcgcatctcg gcattgtctc acgtcgcgcc gcggccgccc gcgccgcgca gctcgtcgtg
     3841 gacatccgag ccaacaacga cgcgatccac gccggaagcg cgcaggtcgt gccgaactcg
     3901 acaagatcac cggatgaacg tgaacagcag cggcccgagg cgccatctag tccaacggcg
     3961 gcccagcacc ccgaccgctc gacaatgaac cgctccaccc ggcatcgccg ccgcacgctc
     4021 gcccctttgc gcggacacga acgggacgca cgccggcttc ggcacctaag ggattcgagg
     4081 atggcagtga cgaatcagac gcgatcaaag ctcgcgcgac gctactcgat ccgggtgttt
     4141 ctatggtgcc agaagattag caagaaatgt ttgcacagcg accacactcg ggattttaag
     4201 tgcttcatcc tgaaaccagc ggttgtcttg aatcgatacc tgaatcgact ttccaggacg
     4261 ccggaattgt tggctccaac cagaaggtta acgcgtccga gatcctcgag cgcgaactcg
     4321 aagaggcccc ggtagcggcc gaccttcagt tggctgatgt gcacacgcat ggcttcctgg
     4381 tggctcttgt accatgttcc atccaggaag ctgaaggacg agagctgagt tcccggacag
     4441 ttaggccggc tggcggctgg ggcctcgaat ttacttcgtc gacgcctagc taggtagcgt
     4501 cgcttagaac cgacgctacc acgctccccg ggtgtggccc cgaacgtagc ggccgacggc
     4561 gacagggtcc aggcggacaa cgtccggcac caggatctac ctccagatgt ggcaacgcat
     4621 ccctcagcga ggtcgcccaa gctgctccaa atcgcagtcg caataatcac tgcttcgcgc
     4681 tttctttgaa ggacgcgaac ggctggtcgt ggccgatgat ggctgatgtg tccgaatcgc
     4741 aggctcaata tctcgatcac cattgttgcc gttccgaacc tggcctacag tatcgagctc
     4801 tgccatggac caacgctggt gcagcagcga tgtcacggtc cacagatgcc tcatcgggtt
     4861 cgcgtgggcc acggcgcaga gccgtgaacg ccggctctta tttatgacac caagaaatgg
     4921 gggtgtctcg gcggtagtag ggccgagcct cgggatcgcg cttcagtgcc tgcggagcag
     4981 gtcgccgagg gtgtcgcgga cggcggggtt ggtctcgatc tcgtggtgct ggcggaggat
     5041 cgggaggacg tcggcgttgt ccatctgggt cagggctgcg taggcggcct cgcgcaggcc
     5101 gccggtctcc tggcgcagca ccatgacgag gaactcgccg gcctcgacgg tctggatctt
     5161 gccgatcgac tgcagcgcgg cggcgcggac gcggggatcg ctggtctcgc ggtagatccg
     5221 ggccagcggg ttgaaggcgt gcgggaagtg caggccggcg atcgcgacga gcgcggcctc
     5281 gcggacctgt gcgtcggggt cggtgagggc cttgcgcacg atgacgaacg agcgcttgaa
     5341 gtagaggtag cgcagggcgc ggaccgcggc gcgccggatg acggcgtcgc cgctggcgaa
     5401 cagcttctcg agcggggaca gcgcggcgta ggactggagc tcgccgagca gctcggcgac
     5461 gtggaccagg gtctcgggct cggcctgggc gcggcgggtc tgggcgtcgg cgagggtgag
     5521 caggacgacg agggcgcggc ggcggatgcc gttggggtag cgcaggtcgc cgacgatcga
     5581 ggcggcgacg gcgtggggat cgccgtccat ctcccactcg aggagatcga cgaaccagat
     5641 gtcggcgtag gcatgttgct gcttgaggta gtcggggagg ccgggcagct cgaccggcgg
     5701 cgcggcgagg ccgcggtagc gctgggcgat ggtggcgaac cgcttcttgg cgcgctcggg
     5761 cagctcgagg ccggcgagct tgtcgaagca ctcgcggacc gcggggtagt cgccgaccgc
     5821 cgagtgctgg gaggcggcgg cgagcagcgc gttctcgacc atctggaccg gcacgccggt
     5881 ctcgacgaac ttgtcggcgc acttcatcca ggtcagcgcg gacttgtgct ggtagtggcg
     5941 gtcgtagggc agcccggcgc gcgcggcgaa cgcggcggcc tcctggtaga gcgtcgcggc
     6001 ggcgtggagc tcgccgcgct cgagcgcgag cttgatgaag tcctcgtagt actgcaggac
     6061 gtagaacttg aggttgtcgt cgcgcagcac gcggatgcag ttgacgtagc cctcggcgag
     6121 gttctcgaac tgcgccgact ccttgccgag cttgagcagg atctggaagc agtcgaacgc
     6181 gcgctcgagc tcgccggtct gctcgaagtc gtcggcgacc tgctcgagct tg
//
LOCUS       DASVOR010001388         3473 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_569953,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001388 DASVOR010000000
VERSION     DASVOR010001388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3473
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569953"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VF516_26385"
     CDS             <1..624
                     /locus_tag="VF516_26385"
                     /inference="COORDINATES: protein motif:HMM:NF021385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8111296.1"
                     /translation="VEQLLTADECGALAALYPGEGFRSRVVMAAHGFGRGEYQYFAYP
                     LPPLVAELRASWYPRLAPIADRWAAALGGAAFPADHASFLARCHAAGQLRPTPLLLRY
                     GESDYNCLHQDLYGEHVFPLQVAILLSQPGRDFTGGEFVMTEQRPRMQSRPMVVPLAQ
                     GDAVVFAVHHRPVQGTRGVYRVNLRHGVSRVRAGQRHTLGVIFHDAA"
     gene            complement(653..3364)
                     /locus_tag="VF516_26390"
     CDS             complement(653..3364)
                     /locus_tag="VF516_26390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX8111297.1"
                     /translation="MRIVLAVLGLVACSPPAPPVQPSPPVAAPTRAAPPDRGLEPPQP
                     TLRPPRNFLPTSYAATLAIDPAAAGFDGTIAITGEVAQRSSVIWLHGHQLAIKRASAA
                     RSGAEVALTATPHGDELLELRAQAPLEPGTWTLAIDYTGVYDALNTTGAFKQVVRGVP
                     YVYSQFEALYARRVFPCFDEPDSKVPWQLTLDVPADQIAVSNTPIVHEEARAGGKKRV
                     TFARTKPLSSYLIAFGVGPFEVIDAGKTRRGTPVRILAMKGRAGETVWAARTSARLID
                     LEEEFFGSPYPYEKMDMLAIPITVGFGAMENAGLITFTETLILLDPRPGSRRREHEWI
                     RVAAHELAHQWFGDLVTTAWWDDIWLNEGFASWVAAKIAARFEPAWREELAEVGDRAA
                     ALDDDSLVSARQVRQPIVTPDDILNAFDGITYNKGAALLNMFESFLGHDVFLRGVRHY
                     IAEHTFGNATSGEFAAAISQAAGVDVGAAFSAFLEQPGAPEITAAVVCDRDRPPRVAL
                     SQQRYVPPGSPTPPATRPWIVPVCVAFDRGGKRAEACGMLSAATGELELPAPSCPRWV
                     MPNLGGHGYYRVAYTGAQVTALRDQAWPQLAPTERSAVYIDAASAATLGKLPLALALS
                     FVPRQLAAGDRFSVGEAIGLPLGLRPFVPDELRPRYESWLRTAFGPAAHKAGLSPQDS
                     DSIDVETARDNLVHVVAGIGRDPALNAEALKLSEHWRDLPEGIRQSVLALAARASPAA
                     FDRLFQEVFSEEDRGRRGALITALASVRDVRQQTAALGLVLDDRLDIRDTAGVFFGAQ
                     EETNRVVAQRYFQDHKDAILKRLPSDGTTDGQAWLAFVFTASCSPERRAEIADYVSRT
                     FAGMPGGARTVRQALEGMDHCIAQRQLLELEIRGWLGGKPDSIRAAK"
BASE COUNT          460 a         1248 c         1285 g          480 t
ORIGIN      
        1 gtcgagcagc tgctcaccgc cgacgagtgc ggcgcgctgg ccgcgctcta cccgggcgag
       61 ggcttccgca gccgggtcgt gatggcggcg cacgggttcg gccgtggcga gtaccagtac
      121 ttcgcgtatc cgctgccgcc gctggtcgcc gagctgcgcg ccagctggta tccgcggctg
      181 gcgccgatcg ccgaccgctg ggccgccgcg ctcggcggtg ccgcgttccc cgccgatcac
      241 gcgagcttcc tcgcgcgctg ccacgccgcg ggccagctcc ggccgacacc gctgctgctg
      301 cgctacggcg agagcgacta caactgcctg caccaggatc tctacggcga gcacgtgttc
      361 ccgctccagg tcgcgatcct gctgtcgcag cccgggcgcg acttcactgg cggggagttc
      421 gtgatgaccg agcagcggcc gcgcatgcag tcgcggccga tggtggtgcc gctcgcgcag
      481 ggcgacgcgg tggtgttcgc ggtgcaccat cgcccggtgc agggcacgcg cggcgtctat
      541 cgcgtcaacc tgcgacacgg cgtgagccgg gtgcgcgcgg gacagcgcca cacgctcggc
      601 gtcatcttcc atgacgccgc gtgaccgcgg cgagctcgac cccgcgtgat cgctacttcg
      661 ccgcgcgtat cgagtcgggc ttgccgccga gccagccgcg gatctcgagt tcgagcagct
      721 ggcgctgcgc gatgcagtgg tccatcccct cgagcgcttg ccgcacggtg cgcgccccgc
      781 ccggcatgcc ggcgaacgtc cggctcacgt agtcggcgat ctcggcgcgg cgctcgggcg
      841 agcagctcgc ggtgaacacg aacgccagcc aggcctgccc gtccgtggtg ccgtccgacg
      901 ggagccgctt gaggatcgcg tccttgtggt cctggaagta gcgctgcgcc accacacggt
      961 tggtctcctc ctgggcgccg aagaacacgc ccgcggtgtc gcggatgtcg aggcggtcgt
     1021 cgagcacgag cccgagcgcc gcggtctgct gccggacgtc gcgcacgctg gccagcgcgg
     1081 tgatcagagc gccgcgccgc ccgcgatcct cctcgctgaa cacctcctgg aacaggcggt
     1141 cgaacgccgc cgggctggcg cgcgcggcga gcgcgagcac gctctggcgg ataccctccg
     1201 gcaggtcgcg ccagtgctcg gacagcttga gcgcctcggc gttgagcgcc ggatcgcgcc
     1261 cgatcccggc gacgacatgg accaggttgt cgcgcgcggt ctcgacgtcg atgctgtcgc
     1321 tgtcctgggg cgacagcccc gccttgtgcg ccgccgggcc gaacgcggtg cgcagccagc
     1381 tctcgtagcg cgggcggagc tcgtcgggga cgaacggtcg caggccgagc ggcagaccga
     1441 tcgcctcgcc gaccgagaac cggtcgccgg ccgcgagctg ccggggcacg aacgacagcg
     1501 cgagcgccag cggcagcttg ccgagcgtcg ccgcgctcgc ggcgtcgatg tacaccgccg
     1561 agcgctcggt cggcgcgagc tgcggccacg cctgatcgcg cagcgcggtg acctgcgcgc
     1621 cggtgtacgc gacgcggtaa tagccgtggc cgccgaggtt gggcatcacc cagcgcgggc
     1681 acgacggcgc cgggagctcg agctcgccgg tcgcggccga cagcatgccg caggcctcgg
     1741 cgcgcttgcc gccgcgatcg aacgcgacgc acaccgggac gatccacggc cgggtggcgg
     1801 gcggcgtcgg cgaacccggc gggacgtagc gctgctggct cagcgcgacg cgcggtggcc
     1861 ggtcgcgatc acagaccacc gccgcggtga tctccggggc gcccggctgc tcgaggaagg
     1921 ccgagaacgc ggcgccgacg tcgacgccgg cggcctggct gatcgcggcg gcgaactcgc
     1981 ccgaggtcgc gttgccgaac gtgtgctcgg cgatgtaatg gcgcacgccg cgcaggaaca
     2041 cgtcgtggcc gaggaagctc tcgaacatgt tgagcagggc cgcgcccttg ttgtaggtga
     2101 tgccgtcgaa cgcgttgagg atgtcgtcgg gcgtgacgat cggctggcgg acctggcgcg
     2161 cgctgaccag gctgtcgtcg tcgagcgcgg cggcgcgatc gccgacctcg gccagctcct
     2221 cgcgccacgc cggctcgaac cgcgccgcga tcttggccgc gacccagctc gcgaacccct
     2281 cgttgagcca gatgtcgtcc caccacgccg tggtcaccag atcgccgaac cactggtgcg
     2341 cgagctcgtg cgcggcgacc cggatccact cgtgctcgcg ccggcgcgag ccggggcggg
     2401 ggtcgagcag gatcagggtc tcggtgaacg tgatcagccc cgcgttctcc atcgcgccga
     2461 acccgacggt gatcgggatc gccagcatgt ccatcttctc gtacgggtag ggcgagccga
     2521 agaactcctc ctcgagatcg atcagccgcg cgctggtcct ggccgcccac accgtctcgc
     2581 cggcgcggcc cttcatcgcg aggatccgga ccggcgtgcc gcgccgggtc ttgccggcgt
     2641 cgatcacttc gaacggcccg acgccgaacg cgatcaggta gctcgatagg ggcttggtcc
     2701 gcgcgaacgt cacgcgcttc ttgccgccgg cgcgcgcctc ctcgtgcacg atcggcgtgt
     2761 tgctcaccgc gatctgatcc gccgggacgt cgagcgtcag ctgccacggc accttgctgt
     2821 cgggctcgtc gaagcacggg aacacgcggc gggcgtacag cgcctcgaac tgcgagtaca
     2881 cgtagggcac gccgcgcacg acctgcttga acgcgcccgt ggtgttgagc gcgtcgtaga
     2941 cccccgtgta gtcgatcgcg agcgtccagg tcccgggctc gagcggggcc tgcgcgcgca
     3001 gctcgaggag ctcgtcgccg tgcggcgtcg cggtgagcgc gacctcggcg ccgctgcgcg
     3061 cggcggacgc gcgcttgatc gcgagctggt ggccgtgcag ccagatcacc gacgagcgct
     3121 gcgcgacctc gccggtgatc gcgatcgtgc cgtcgaaccc ggccgccgcc ggatcgatcg
     3181 ccagggtcgc ggcgtagctc gtcggcagga agttgcgcgg cgggcgcagc gtgggctggg
     3241 gcggctcgag gccgcgatcc ggcggtgcgg cgcgggtcgg cgcggcgacc gggggcgatg
     3301 gctggaccgg cggtgcagga ggcgagcacg cgacgaggcc gagcacggcg agaacgatcc
     3361 gcatggccac actgtatcgc cgacgcgctc gcggatccga gcaaacgcaa atgcgacagg
     3421 tgcgacgtcc ggcgacgccg cacctgccgc ttcgatcgat cacgcagcga tcg
//
LOCUS       DASVOR010001389         5499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_569962,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001389 DASVOR010000000
VERSION     DASVOR010001389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569962"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VF516_26395"
     CDS             <1..759
                     /locus_tag="VF516_26395"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8111298.1"
                     /translation="RSRAAAEAARRATAARDELVAIVSHDLRNPLATITMGADLLPEL
                     DDNPRTRPHLAAIGNAARLMQHLIDQLLDVAKLEHGTLGLAFERCEVDAVLGAVQSLF
                     QVRAVEAGVELTAIGEPGVAVTADRERVVQVLSNLVANALKFTPRGGRIAVTGGRDGS
                     RVRFEVSDTGPGIAEDQIPRLFERYWQGRSSPRGSLGLGLYICKQLVAAHGGDIGVRS
                     RLGDGSTFWFTLSAAGVAITAPGGTRPDRRPPRA"
     gene            complement(710..856)
                     /locus_tag="VF516_26400"
     CDS             complement(710..856)
                     /locus_tag="VF516_26400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111299.1"
                     /translation="MSFRSPLSYFHNGIAATLEDVVALYQKSLGFVFTPEEAADLVAF
                     LQAL"
     gene            complement(1382..3709)
                     /locus_tag="VF516_26405"
     CDS             complement(1382..3709)
                     /locus_tag="VF516_26405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8111300.1"
                     /translation="MTSPATEAKVPEIVEEALSQIRVLLDTLFETAEDAIFLMHGPRF
                     VDCNPITLRMFGCKAKDDILGKTPVQFSPDRQPDGASSADQARDLIAAAMAGAPQHVE
                     WRYHRLDGTLFDVEINLNRCFVRAAPFLIAVVHDITARKRAEAALLQEKQFSERLVDS
                     LPGTFYLYDSQLRLRRWNKNPETQMGYTAEELHGKTIGDFAATEAERDRVLTAARRLL
                     ERGEVEIQEISLLHKDGSAVPYLVSGARVDSPDGPMVLGVGLNLTPLVQAKKALAASE
                     RNYRELFNATHEGIFIHDEAGRVLDVNERGCAMFGLDASEARRSLLVELTPGEPPYAQ
                     RDASEKLRCAISEGPQVFDWQCGSVDGTVFWSEIALRAFRIDGEVRVIASVRDVTERK
                     RAGFERERLIAESQAANRAKDEFMAVLSHELRTPLAAIQASIDLLLCLPTLDEPRAQS
                     ALRIIERNVKLQTRLVNDLLDMSRLIRGRLTIVRAPARLDEAVLSAADTCRDDAARAQ
                     VTLETRAEAELWANADATRIQQMVINLIDNAIKFTPNGGRITVSVTAKERHGYVIVED
                     TGVGIAPDRLSEIFEMFRQGEVAARRAPGLGIGLALVKSIVDLHGGRVWAESAGPGRG
                     SRFIVELPLYEAPTTRTTHEVPAPERAILKVLLVEDNPDTRTMLAETLSQLDYMVLPA
                     ESAEAGLEILAREPVDVIVADMGLPGMDGYEFLRQARHLPAAARVPALALTGYGQDSD
                     VRRAREAGYAQHVVKPANVEVIDRHIRLLVGGREG"
     gene            complement(3838..4014)
                     /locus_tag="VF516_26410"
     CDS             complement(3838..4014)
                     /locus_tag="VF516_26410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111301.1"
                     /translation="MELGSRGRELELGLDQVALGGRVIDGVDLGIAGYALWEVSDDRG
                     PDLLASWYLRCGRG"
     gene            complement(4054..4686)
                     /locus_tag="VF516_26415"
     CDS             complement(4054..4686)
                     /locus_tag="VF516_26415"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8111302.1"
                     /translation="MKATSDADHRTRVGRERSARTENRILEAALRVFAEHGPDAPVID
                     DFVQAAGIARGTFYNHFKTVEELLHATSEWTTRETIKAIEASIAGIEGPTLRLGVGLR
                     LFFARAQRDPVWCRFVAKVWKVGGLELPMRDLEVGIRLGHLRVPSRDAARDLILGAVR
                     AALERIGDHRAPAGFSEHMTEICLHALRADPRRITAALAHELPPLAERSE"
     gene            complement(4683..>5499)
                     /locus_tag="VF516_26420"
     CDS             complement(4683..>5499)
                     /locus_tag="VF516_26420"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8111303.1"
                     /translation="AARAPTLDVAAATLLSPVTAPARVICQGANYRQHMIESGVDPDA
                     KRFNMFFEKSDASISAPAGAIVRPAHVRLLDYEIELALVMRKPISGPITVTAETLHEY
                     VFGLTIANDVSARDVQLPQTQFFKGKSYRGFCPLGPYLAVLEQADLPYLDQLVLTLEV
                     NGTVRQRDSTANLVYKPAETLTELSTFSDLSPGDVLLTGTPAGCALRAPRRLVRSLMQ
                     LVLPEPQLWRAFVRAQGNRPEYLRPGDAMRATIASADRAIDLGEQRHTIEGAP"
BASE COUNT          866 a         1893 c         1887 g          853 t
ORIGIN      
        1 cgatcgcgcg ccgccgccga ggccgcccgc cgggcgaccg ccgcgcgcga cgagctggtc
       61 gcgatcgtct cgcacgacct gcgcaacccg ctcgcgacga tcaccatggg cgccgatctg
      121 ctccccgagc tcgacgacaa cccgcggacc cgcccgcacc tcgcggcgat cggcaacgcc
      181 gcccgcctga tgcagcacct gatcgaccag ctgctcgacg tcgccaagct ggagcacggg
      241 acactcgggc tggcgttcga gcgctgcgag gtcgacgccg tgctcggcgc ggtgcagtcg
      301 ctgttccagg tccgggcggt cgaggccggg gtcgagctga ccgcgatcgg cgagccgggg
      361 gtggcggtca ccgcagatcg cgagcgcgtg gtccaggtgc tgtcgaatct ggtcgccaac
      421 gcgctcaagt tcacgccgcg cggcggccgg atcgcggtca ccggcgggcg cgacgggtcg
      481 cgcgtccggt tcgaggtctc ggacaccggc cccgggatcg ccgaggacca gatcccgcgg
      541 ctgttcgagc gctactggca gggccgatcg tcgccgcgcg gcagcctcgg cctcggcctg
      601 tacatctgca agcagctcgt cgcggcccac ggcggggaca tcggcgtgcg cagccggctc
      661 ggcgacggct cgacgttctg gttcacgttg tctgccgccg gggtagcgat cacagcgcct
      721 ggaggaacgc gaccagatcg gcggcctcct cgggcgtgaa cacgaagccg agcgacttct
      781 ggtagagggc gacgacgtcc tcgagcgtgg ccgcgatgcc gttgtggaag tacgaaaggg
      841 gcgatcgaaa actcaagtgt tgtcgacgca ctgcaaccct ttcaagcgct tccgcgcggc
      901 cgaatcccgc tgaggtccgc tcggtgccgc tgagtcccgc cggttctggc aaagccgctg
      961 gcaaagccgg agacacgttc gcccgcgagt cgcgcggcac gctgcgaacg ccaaacgcgc
     1021 gtcttccagc cgcgccgcga gcgccaggca gcctccaagc aaggccagcc actgcgagcg
     1081 tctccatgcg caacctgggg agcacgccct gtgccggatt tcggactccc aagctatcga
     1141 gataaccgga cgacgatccc ggcccgccgt ggccccggcg ccgaccaggt gcacgcccga
     1201 aggtccaggg tcggatgtcg gtttgcaaac gcgcgcgaag gcggccccgc gccaagccga
     1261 ccgcgagcgc gccgctccgg ccgttccagg ccgaaccgcg agcgcgctgc gacaccggcg
     1321 acgaccagat ctgaggtcgg accgcttacc gcgttaaccc gcaggcgcca cgggcagcgc
     1381 gtcacccctc ccgtccgccc acgagcaagc gaatatgccg gtcgatcacc tcgacattgg
     1441 caggcttgac gacgtgctgt gcgtaacccg cttcgcgcgc ccggcgcacg tcactgtcct
     1501 gcccgtaccc ggtcaacgcc agcgcaggaa cacgcgctgc ggcgggcaga tggcgggcct
     1561 ggcgaagaaa ttcgtagcca tccatgcccg ggagccccat gtcagcgacg atcacgtcca
     1621 ccggctcgcg cgcgaggatc tcgaggcccg cctcggcgct ctccgcgggc agtaccatgt
     1681 agtcgagctg cgagagcgtc tcggcgagca tggtgcgcgt gtcagggttg tcttcgacca
     1741 ggagcacctt caggatggct cgttccgggg caggaacctc gtgtgtggtg cgagttgtgg
     1801 gagcctcata caggggcagc tcgacgatga accggctgcc gcgcccgggg ccggcgctct
     1861 cggcccacac acgaccgccg tgcaggtcga cgatcgactt caccagcgcc agcccgatgc
     1921 ccaggccggg cgccctgcgc gcagcgactt caccctgccg gaacatctcg aagatctccg
     1981 acaggcgatc gggagcgatg cccacgccgg tgtcttcgac gatcacgtag ccgtggcgct
     2041 ccttcgcggt gaccgacacc gtgatccgtc cgccgttcgg cgtgaacttg atcgcgttgt
     2101 cgatcaggtt gatgaccatc tgctggatgc gcgtggcgtc ggcgttggcc caaagctccg
     2161 cctcggcgcg cgtctcgagc gtcacctggg cgcgcgcggc gtcgtcacgg caggtgtcgg
     2221 cggccgagag cacagcctcg tcgagccgcg ccggagcgcg aacgattgtg agcctgcctc
     2281 gaatgaggcg cgacatgtcg aggaggtcat tcacgagccg cgtctgcagc ttcacgttgc
     2341 gttcgatgat tcgcagcgcg gactgcgctc gtggctcgtc cagcgtcggc aagcacagca
     2401 ggagatcgat gctggcctgg atcgctgcca ggggggtgcg cagctcgtgc gacagcactg
     2461 ccatgaactc gtcctttgcg cggtttgcgg cttgcgactc cgctatcaga cgctcgcgct
     2521 cgaagcctgc gcgcttgcgt tcggtgacgt cgcgaaccga ggcgatgacg cgtacctcgc
     2581 catcgatgcg gaacgcgcgc aaggcgatct cggaccagaa gacggtgcca tcgacgctcc
     2641 cgcactgcca gtcgaagacc tgcggccctt cggagatggc gcagcggagc ttctcggacg
     2701 catcgcgctg cgcgtagggc ggctcgcccg gggtcaactc gaccaggagc gagcgacggg
     2761 cctcggaggc gtcgaggccg aacatggcgc agccgcgctc gttgacgtcc agcacgcgcc
     2821 cggcttcatc gtgaatgaag atcccctcgt gggtggcgtt gaagagctcc cggtaattgc
     2881 gttcgctcgc tgccagtgcc ttcttggcct ggacgagtgg ggtgaggttg aggccgacac
     2941 ccagaaccat gggaccgtcc ggagaatcga cgcgcgctcc tgacaccagg tacggaacgg
     3001 cggagccatc cttgtgcagc agcgagatct cctgtatctc gacctcgccg cgctccagga
     3061 ggcggcgcgc cgccgtgagt acccggtccc gctccgcctc ggttgccgcg aagtccccta
     3121 tcgtcttgcc gtgcagctcc tcggccgtgt aacccatctg cgtctcgggg ttcttgttcc
     3181 agcgccgcaa acggagttgc gaatcataga ggtagaatgt accgggaagg gagtcaacga
     3241 gacgctcgga gaactgcttc tcctggagca gcgcggcttc ggctcgcttg cgcgcggtga
     3301 tgtcgtgcac caccgcgatc agaaacgggg ccgcacgcac gaagcaacgg ttcaggttga
     3361 tttcgacgtc gaacagcgtg ccgtcgagcc ggtggtatcg ccactccacg tgctggggag
     3421 cgccggccat ggccgccgcg atgagatcgc gggcctggtc tgccgacgaa gcaccatcgg
     3481 gctgccggtc cggagagaac tggacaggag tttttcccag gatgtcgtcc tttgccttgc
     3541 atccgaacat ccgcaaggtg atggggttgc agtccacgaa acgcggcccg tgcatgagaa
     3601 agatggcatc ctcggccgtc tcgaacagcg tatcgagcaa gacacgtatc tgagaaaggg
     3661 cctcctcgac gatctccggc accttggcct cggtggctgg cgacgtcacg gcgcaacttg
     3721 gtgcacgatg agtgccacga ggtctctgac gtcattgaca ccaccacgga gagttcgagc
     3781 acgagaatgg ttgctaccgg cctcgccttg ctggcgatgt cccacgcaac cgcacgctca
     3841 gccgcgacca catcgcaggt accaggaggc caggaggtcc ggcccgcgat cgtcgctgac
     3901 ctcccagagc gcgtagcccg cgatgccgag atcgacgccg tctatcacgc gcccaccgag
     3961 ggcaacctgg tccaggccga gttccagctc gcgcccgcgt gagcccagct ccactgcgcg
     4021 gtcacccagg agtagcccgc ggctcacccg cggtcattcg ctccgctcgg cgagcggcgg
     4081 cagctcgtgc gcgagcgccg cggtgatccg gcgcggatcc gcgcgcagtg cgtgcaggca
     4141 gatctcggtc atgtgctcgc tgaagcccgc cggcgcccgg tgatcgccga tgcgctccag
     4201 cgccgcgcgg accgcgccga ggatcaggtc gcgcgccgca tcgcggctgg ggacgcgtaa
     4261 gtgtccgagg cggatcccca cctcgagatc gcgcatcggc agctcgagcc cgccgacctt
     4321 ccataccttc gccacgaagc ggcaccacac cgggtcgcgc tgcgcccgcg cgaagaacag
     4381 ccggagcccc acgccgagcc gcagggtggg accctcgatc cccgcgatcg acgcctcgat
     4441 cgccttgatg gtctcgcggg tcgtccactc cgaggttgca tggagcagct cctcgacggt
     4501 cttgaagtgg ttgtagaacg tcccgcgcgc gatgcccgcc gcctgcacga aatcgtcgat
     4561 cacaggtgcg tcgggcccgt gctcggcgaa cacgcgcagc gccgcctcga ggatgcggtt
     4621 ctcggtgcgc gcgctgcgct cccggccgac ccgcgtccgg tgatcggcgt cgctcgtcgc
     4681 cttcatggcg cgccctcgat cgtgtggcgc tgctcgccga gatcgatcgc gcgatccgcg
     4741 ctggcgatcg tcgcgcgcat cgcgtcgcct gggcgcaggt actcggggcg attgccctgc
     4801 gcgcgcacga acgcgcgcca cagctggggc tcgggcagca cgagctgcat caggctccgc
     4861 accaggcgcc tgggagcgcg cagcgcgcaa cccgccggcg tgccggtgag gaggacgtcc
     4921 cccggggaca ggtcgctgaa cgtcgagagc tcggtgaggg tctcggcggg tttgtagacc
     4981 aggttcgcgg tggagtcgcg ctggcgcacg gtgccgttga cctccagcgt cagcacgagc
     5041 tgatcgagat atgggagatc ggcctgctcg aggaccgcga ggtatggtcc gagcgggcag
     5101 aagccgcgat agctcttgcc cttgaagaac tgggtctgtg gcagctgcac gtcgcgcgcg
     5161 gatacgtcgt tggcgatcgt caagccgaac acgtactcgt gcagcgtctc cgctgtgacc
     5221 gtgatcgggc cagagatcgg cttccgcatg acgagtgcga gttcgatctc gtagtcgagc
     5281 agccggacgt gcgccgggcg cacaatggcg cccgccggcg cgctgatcga ggcgtccgat
     5341 ttctcgaaga acatgttgaa ccgtttggca tcgggatcga cgccggactc gatcatgtgc
     5401 tgccggtagt tcgcgccctg gcagatcacg cgggccggcg cggtcaccgg cgacagcagc
     5461 gtcgccgcgg ccacgtcgag cgtcggagcg cgcgcggcg
//
LOCUS       DASVOR010001390         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_510953,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001390 DASVOR010000000
VERSION     DASVOR010001390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510953"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..1101)
                     /gene="hemE"
                     /locus_tag="VF516_26425"
     CDS             complement(55..1101)
                     /gene="hemE"
                     /locus_tag="VF516_26425"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEX8111304.1"
                     /translation="MADSLFLRACRRQPVERTPIWMMRQAGRYLPAYRAVRARASFLD
                     LCHNPELACEVTLQPIDEFGFDAAILFSDILITLPAMGIDIAFPEAGPKIAAPIRDHA
                     AIEALHVPDPEAELPFVMEAVRKIKAALAGRVPLIGFAGAPLTMLTYAVEGGGSKDYA
                     HTKAMLFGAPASAHALLDKLARTCATHLEAQVAAGADAVQIFDSWGGIVSPGDFREYP
                     LRYARQVIEQLRASPVVASRGVPIIYFVNGCAPYLEDYASSGADVLGVDWRVGIDEVR
                     RRVGDGVALQGNLDPGALFAAPGEIRARVADILARAGATGHVFNLGHGVLPETDPEHV
                     RAMVTAVKELSDRA"
     gene            1137..>1946
                     /locus_tag="VF516_26430"
     CDS             1137..>1946
                     /locus_tag="VF516_26430"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8111305.1"
                     /translation="MRWPAVFAVLAALAGCGENAASAAFDGLPLDGDFTVGVDAPVHV
                     ARDRHGIAHILAGSLGDAAFVQGYVTAHDRLPQMDVLRRLGAGTLAELYGAIDPSVID
                     LDLEMRLYRMVPLATATLGALQTSQLPVDRDVVQLVQRFADGVNAYVRDLQRGYWTID
                     PSLGTGVAAVYDPAAFRPWSPIDSLVLVRFEAFAQSWSAPREITATELYQKLRATFDD
                     ADPGNLAAVARRGISRDVLRLAPVGTASTIAGFPNVAVDTGSRSDGSDPPPS"
BASE COUNT          258 a          706 c          722 g          260 t
ORIGIN      
        1 gggcgcccag ggcgagtgcg gcggtgagcg cggcgacggc cccgaccgcg gcgctcatgc
       61 ccggtccgac agctccttga ccgcggtgac catcgcccgg acgtgctcgg gatcggtctc
      121 gggcagcacg ccgtgcccga ggttgaagac gtggccggtc gcgccggcgc gcgcgaggat
      181 gtcggcgacc cgggcgcgga tctcgccggg ggccgcgaac agcgcgccgg ggtcgaggtt
      241 gccctgcagc gcgacgccgt cgccgacccg ccggcggacc tcgtcgatcc cgacccgcca
      301 gtcgaccccg agcacgtcgg cgccgctcga cgcgtagtcc tcgaggtaag gcgcgcagcc
      361 gttgacgaag tagatgatcg gcacgccccg gctcgcgacc accggcgacg cgcgcagctg
      421 ctcgatgacc tgccgggcgt agcgcagcgg gtactcgcgg aagtcgccgg gcgacacgat
      481 gccgccccag ctgtcgaaga tctgcacggc gtcggcgccg gcggcgacct gggcctcgag
      541 gtgggtcgcg caggtccgcg ccagcttgtc gagcagcgcg tgggccgacg ccggcgcgcc
      601 gaacagcatc gccttggtat gggcgtagtc cttggagccg ccgccctcga ccgcgtaggt
      661 cagcatggtg agcggggcgc ccgcgaagcc gatcagcggc acccgtccgg cgagcgcggc
      721 cttgatcttg cgcacggcct ccatcacgaa cggcagctcg gcctcgggat cggggacgtg
      781 cagcgcctcg atcgcggcgt gatcgcggat cggcgcggcg atcttgggcc cggcctcggg
      841 gaacgcgatg tcgatcccca tcgccggcag cgtgatcagg atgtcggaga acaggatcgc
      901 ggcgtcgaac ccgaactcgt cgatcggctg gagcgtgacc tcgcacgcga gctcggggtt
      961 gtgacacaga tcgaggaacg acgccctcgc gcgcaccgcg cgatacgccg gcaggtagcg
     1021 gcctgcctgg cgcatcatcc agatcggggt gcgctccacg ggttgccggc gacacgcgcg
     1081 caggaacaga gaatcggcca tcgcgagcat cctacccgcc gaggtatacc cgcgccatgc
     1141 gttggcccgc cgtcttcgcc gtcctcgccg cgctggcggg ctgcggcgag aacgcggcga
     1201 gcgccgcgtt cgacggcctg ccgctcgacg gcgacttcac ggtcggggtc gacgcgccgg
     1261 tgcacgtcgc ccgcgaccgc cacggcatcg cgcacatcct ggccggctcg ctcggggacg
     1321 cggcgttcgt ccagggctac gtcacggcgc acgaccggct gccgcagatg gacgtcctgc
     1381 gccggctggg cgccggcacg ctcgccgagc tgtacggcgc gatcgatccg tcggtgatcg
     1441 acctcgatct cgagatgcgg ctgtaccgga tggtgccgct ggcgaccgcg acgctgggcg
     1501 cgctgcagac gtcacagctc ccggtcgacc gcgacgtcgt gcagctggtg cagcggttcg
     1561 ccgacggcgt gaacgcctac gtccgcgacc tccagcgcgg ctactggacg atcgatccga
     1621 gcctgggcac cggcgtggcc gccgtctacg atccggcggc gttccggccg tggtcgccga
     1681 tcgactcgct ggtgctggtc cggttcgagg cgttcgcgca gtcgtggtcg gcgccgcgcg
     1741 agatcaccgc gaccgagctg taccagaagc tgcgcgcgac gttcgacgac gccgatccgg
     1801 gcaacctcgc cgcggtggcg cgccgcggga tctcgcgcga cgtgctgcgg ctggcgccgg
     1861 tcggcaccgc gtcgacgatc gccgggttcc ccaacgtcgc ggtcgacacc ggctcgcgct
     1921 ccgacggcag cgatccgccg ccgagc
//
LOCUS       DASVOR010001391         2143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_452459,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001391 DASVOR010000000
VERSION     DASVOR010001391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2143
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452459"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..943
                     /locus_tag="VF516_26435"
     CDS             <1..943
                     /locus_tag="VF516_26435"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEX8111306.1"
                     /translation="PVSPNLKTNVAIAGVLALVAGIAIIFLIVFLDRSIKTTGDATQA
                     ADAPVLGIIPQLIAADLGGAADDRARDMYVHENPKSPIAECCRSLRTNILFSAADRQL
                     KTLVVCSANPREGKTTSVLYLGTTMAQSGQRTLVIDTDMRRPRLHRSTGVALAPGLSN
                     LLIGEDIYDHLIQPTEVSNLFILPCGLTPPNPAELLLTKRFEHVLGELARRFDRVILD
                     SPPIQAVTDAVVLSKRVDGAILVVRASKTMRDELRRSARQIRDVGGSIVGVIVNELDA
                     RDSYYGYGGYGYGGYGYGGYYGADADPDPDAKPGEAA"
     gene            complement(1370..1930)
                     /locus_tag="VF516_26440"
     CDS             complement(1370..1930)
                     /locus_tag="VF516_26440"
                     /inference="COORDINATES: protein motif:HMM:NF033542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEX8111307.1"
                     /translation="MTLPERLRTHARASGRELPEHLERQLTMLTQVTEQVQAANKQAR
                     RLATEHPVCRRLMAVPGVGPLTSLRFVAAIDDPSRFPTAHRVQSYVGLTPGEHSSSRR
                     ERKTGITKAGPSELRRCLVQAAWSVMLARRSHPMLEWVRQIAQRRNRPVAVIALARKL
                     AGILFAIWRDGTTYAPKHMTQRSAPT"
BASE COUNT          306 a          771 c          721 g          345 t
ORIGIN      
        1 cccggtgtcg ccgaacctga agaccaacgt cgcgatcgcc ggggtcctcg cgctggtcgc
       61 cggcatcgcg atcatcttcc tgatcgtgtt cctcgaccgc tcgatcaaga ccacgggcga
      121 cgcgacccag gccgccgacg cgccggtgct cggcatcatc ccgcagctca tcgcggccga
      181 cctcggcggc gccgccgacg accgcgcgcg cgacatgtat gtccacgaga accccaagtc
      241 gccgatcgcc gagtgctgcc ggtcgctgcg caccaacatc ctgttctccg ccgccgaccg
      301 ccagctcaag accctcgtgg tgtgcagcgc caacccgcgc gagggcaaga ccacgagcgt
      361 gctctacctc ggcaccacga tggcgcagag cgggcagcgc accctcgtga tcgacaccga
      421 catgcgccgg ccccggctcc accgctcgac cggcgtcgcg ctcgcccccg ggctgtccaa
      481 cctgctgatc ggcgaggaca tctacgacca cctgatccag ccgaccgagg tcagcaacct
      541 gttcatcctg ccgtgcggcc tgaccccgcc caacccggcc gagctcctgc tcaccaagcg
      601 gttcgagcac gtgctcggcg agctcgcccg gcggttcgat cgcgtgatcc tcgactcgcc
      661 gccgatccag gcggtgaccg acgcggtcgt gctgtccaag cgggtcgacg gcgcgatcct
      721 cgtggtccgc gccagcaaga ccatgcgcga cgagctccgc cgctcggcgc gccagatccg
      781 cgacgtcggc ggctcgatcg tcggcgtgat cgtcaacgag ctcgacgcgc gcgacagcta
      841 ctacggctac ggcggctacg gctacggcgg ctacggctac ggcggctact acggcgccga
      901 cgctgatccg gatcccgacg ccaagcccgg cgaagccgcc taggaacttg taggaacttg
      961 cagcgcatag cgctcaggag aacgcgtaca cgcgcctcgc cgtgggctgc tgcgcggatg
     1021 ggcgtcggag ccgcgcccgc cgactcacca ccggcgcctg cgcgagcggc gcgactatgt
     1081 tgtggcggtc gtgtcaagcg cagatctctc ttgtccggat ggttctcctc cggtcggtcg
     1141 ggttggctgc ggttccagga gtggctggcg tgtggattgg aggtgtctgg ctgtagcagt
     1201 cgactattcg cgctctggtg gcgcagcagg gggtccgtcg gcggcggcgc actcgaggta
     1261 acgcggttct cgtcggaagc ccgacgagcc ggaatcgcgc gtctcgcgct caccgcctct
     1321 ggacagccat cacctgggtc cgaggcacag gacgttggtc gggcgagtgc tacgtcggag
     1381 cgctcctctg cgtcatgtgc ttgggtgcgt aggtcgtccc gtcgcgccag atcgcgaaca
     1441 ggatgccggc gagctttcgg gccagcgcga tgaccgcgac gggtcggttg cgccgctgag
     1501 cgatctggcg gacccactcg agcatcggat gcgacctgcg agccagcatc accgaccatg
     1561 ccgcttgtac caggcagcgt cgcagctcgc tcgggccggc cttggtgatc cccgtcttgc
     1621 gttcgcgccg cgagctcgag tgctccccgg gcgtcagccc cacgtaggac tgcacccggt
     1681 gcgcggtcgg aaaccggctc gggtcgtcga tcgctgccac gaagcgcagc gacgtgagtg
     1741 gtccgacgcc gggaacggcc atcagccggc gacacacggg gtgctccgtc gcgagcctgc
     1801 gagcctgctt gttcgctgcc tgcacttgct cggtcacctg ggtcagcatc gtgagctgcc
     1861 gctcgaggtg ctcgggcagc tcgcgcccgc tggctcgcgc gtgggtccgc agtcgctccg
     1921 gcagggtcac catcgtcccg ccgggaggtt tccacagctg ggtccgcagt cagccccgga
     1981 cgttgttgga caggttgcgc tgcgtcgcga cgagcacgtc gcgagcgccg cagaacgact
     2041 tgagctcgcg tgccgctgcc gacctggacc gagggaacat cggtctgcca ggacgcctgg
     2101 ctcagctggc gagcatcccg ctggtcgttc ttgactcccc gat
//
LOCUS       DASVOR010001392         7373 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_570001,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001392 DASVOR010000000
VERSION     DASVOR010001392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7373
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570001"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="VF516_26445"
     CDS             <1..240
                     /locus_tag="VF516_26445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111308.1"
                     /translation="RRARTRRVVGKVAPEAALRAVQDEPATAITATARAPRKPARRGS
                     GMAARPTLQAVAAPAPSTTPSSVEPTSAGEIVGNG"
     gene            complement(538..1287)
                     /locus_tag="VF516_26450"
     CDS             complement(538..1287)
                     /locus_tag="VF516_26450"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8111309.1"
                     /translation="MNQTAWLARPRPDPTARLRLVCFPPAGGNDAMFRTWPPSLPAEV
                     EVCSILLPGRDARRREPTIPRVTPLVKAIADGLAGELTGPFALFGHSTGALVAFELAR
                     ELRRRGQPGPVLLVAAGRPAPSCPVRDPMHALPSSQLTERLRRLGGTPEAVLREPDLM
                     AMFLPIVRADLEVNEVEPYRSEPPLACPIVAYGGAMDERCRRDELVAWGEHTSGGFSY
                     ELFPGGHFFVRTGARELHASLGQALRRVVAP"
     gene            complement(1371..3134)
                     /locus_tag="VF516_26455"
     CDS             complement(1371..3134)
                     /locus_tag="VF516_26455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111310.1"
                     /translation="MGLGVFVLASLLVNGYRFGVSDHAVHLPFLLRTLDPSYLVGDPA
                     VDALAHHPSLFWELQAQLASWLSIEHLYLAIHVVSLAALYAGARALGRALFPGDQGIW
                     ARAIIPALVVVSHETVAGIPSMDGEVLNRTVSNGPLLYALALGAQGAWLPGFAVAGLT
                     FWIHPTTAFHAVVLLWCSALVVRGSHRRVRTAILGPLVFLIAASPLIVKLANHRGAGG
                     VPFPGPPEWWDFVRQHMYFHIYPLDTLWVFGPFVVMYGLLFFAVGRRLLPTAARGYLL
                     GIAVCCGAGGLGTGLLKIPQAMFLHLWESVRFLVFIAAACCAAWVVGRWQGAETRRAR
                     LASVSFAFAFAIDLPFLVPWWTYPPLVHHAIVVTSLVVCAALIAVVRTATVTPNTRVA
                     CHVRPPPWWLGPGLVAAVAMFHFAVGPGDTRPTFTSTHPGVIQHRFVDAPEDVALETW
                     CRTHLPPNALVVLPLDWESLLHFRYGARRAVLATLKDGPESVFDLEYNRSYRHRVEAI
                     CDCRPFDLTRYGDQAGGARLFMEFDVIRDGVEKMTGDRARSLARDFGVTHIVIRASQP
                     VADLGEVVYRDPAYAVVRVAP"
     gene            complement(3229..3993)
                     /locus_tag="VF516_26460"
     CDS             complement(3229..3993)
                     /locus_tag="VF516_26460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8111311.1"
                     /translation="MLAIPCFNEERRLEPRSVSALVRDRQLDVILVDDGSTDRTLAVL
                     EALRDKADPHVSVLALPENRGKAEAVRCGLLAAIDRAAVVGYADADFATPPDELLRLA
                     SLALDSDADAVIGSRISFLGADIVRHPVRRYLGRVFAAAASVVLQRSVYDTQCGAKFF
                     RVTDALRSALATPYISRWAFDVELLGRLWTADPGYSIVEAPLKAWADVRGSKIGPASM
                     VRAGVELARIALELRRRSPRRLMASVGRARRGSWNE"
     gene            4427..>7373
                     /locus_tag="VF516_26465"
     CDS             4427..>7373
                     /locus_tag="VF516_26465"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8111312.1"
                     /translation="MFTPVHFGSLLFTPVHFIKSVFRSHEIALVFSNRPCEEAVPTIA
                     MQSKSKFQSRSQALDIELIHLIEALAAQLLSCSPADIARDVPFRELGLDSAGVTMLGA
                     ELARTLDRPIPVPVMWTHATVRALAGYLAAPTDRTAAGPLTHAPANADEPIALVGLGC
                     RVPASIASPGELWAALLAEVDAVREVPADRWDLARWFSADPEAPGTMTARCGAFLDDV
                     AAFDAGFFRISPREARQIDPQQRLALEVAVEALEDARIVPSRLAGGRTGVFFGAMWHD
                     YALLAGADPRTIEPHSATGWDTSVISARIAYALGLRGPALTVATACSSSLVALHLATQ
                     SLRRGETDLALVGGVNLMLHPLSSVLMSKFGALGPAGRCRAFAAGADGYVRGEGCVVV
                     AARRLSDAVHAGDRIYALIRGTAINNDGASNGIAAPNPVAQIDVLEAAWRDAGIAPAE
                     VGYVEAHGTGTPLGDPIEASALAAVFAQDRQAPLAIGSVKTNFGHLEAAAGLLGLAKA
                     ALSLFHGELPRSLHFDAPNPHIDFDAQRLEVVQARRPWPEGRRLAGVSSFGFGGTNAH
                     VALEQAPFTPRSRPRPRAVAAARGDRGLVWFFSGHGGQWPGMARELIATDAVFRAAIE
                     RCDHALHPVTGWSVVRELAGEPSQSRIEQTRFSEPAIFALQIALAEVLAHAGVRPDAV
                     IGQSIGEIAAAVVSGALTLDDGARVVARWADLVTDHACGTGGILVIDCAAEAAAALIE
                     GRDGLSVAGVLSPRHTCLAGSTLALAELASVARGLDHRIGHVRIDYPCHGPAMAATAE
                     RLAAELGELPAHPAALPLWSTVTAGPLVGPEMSAAYWARNMREPMRIAEAMARLVATR
                     PMTVVELGPHPIIREGLQALFESMARPDCRALASCRRGEPGTAGLATLMHELARDGLI
                     DAPTAEPPSLIVVSGKSPAALRNNCARLAGAIESEGEALGTIAGTLAVARDHHPHRVA
                     VVAADRAHAA"
BASE COUNT         1258 a         2626 c         2504 g          985 t
ORIGIN      
        1 cggcgcgcac ggacgcggcg cgtggtcggc aaggtggccc cggaggccgc gctgcgggcg
       61 gtccaggatg aaccggccac cgcgatcacc gcgaccgcca gggcaccgcg caagcccgcc
      121 cggcgtggca gcggcatggc agccaggccg accctgcagg cggtcgcagc cccggcgccg
      181 agcaccaccc ccagctcggt cgaacccact agcgcgggcg agatcgtggg taacgggtaa
      241 tggccgacgc gttacccaaa agccgaaaag gcttacgccc ctttcgctgg gtggcgtaag
      301 gcgaagattc ctcgtgcgca aaggggactc gaacccccac ggtgttaccc actagcacct
      361 caagcaaggc cgcagcctag attccagata tttgcatcgt tttttgtagc cagaacgcca
      421 attcccatgc agattctgga tcttagactg gataatccgt ctcaccgtac ttgaatccct
      481 gtgctcgttc ccccgcgcgg aagcaggagg tagccggcat ggggtaccgg ccctacctca
      541 gggtgctacg acccgacgga gcgcctggcc gagtgaggcg tggagctcgc gggcgccggt
      601 acgcacgaag aagtggccac cggggaacag ctcgtacgag aacccgccag aggtgtgctc
      661 gccccacgcg acgagctcgt cgcgccggca ccgctcgtcc atcgcgccgc cgtacgcgac
      721 gatcgggcag gccagcggcg gctcggaccg gtagggctcg acctcgttga cctccagatc
      781 ggcgcgcacg atcggcagga acatcgccat caggtcgggc tcgcggagca cggcctcggg
      841 cgtgccaccg agccggcgca agcgctccgt aagctgggag gacggcagcg cgtgcatcgg
      901 atcgcgcacg gggcaactcg gtgcgggccg accggcggcg acgagcagca ctggaccagg
      961 ctggccacgc cgccgcaact cgcgggcgag ctcgaacgcc acgagcgcac cggtactgtg
     1021 tccgaacagc gcaaacggcc cggtgagctc gccggcgagg ccatcggcga tcgccttgac
     1081 cagcggagtc actcgcggga tcgtcggctc ccgacggcgc gcgtcgcggc cgggcagcag
     1141 gatcgagcag acctcgacct cggccggcag cgatggcggc caggtgcgaa acatcgcatc
     1201 gttgccgccg gccggcggga agcacacgag gcgcagccgc gcggtcgggt cgggccgcgg
     1261 acgagcgagc caggctgtct ggttcacgca tccctcacga tcatgcaggt gacggcgcgt
     1321 agttctcgta ggaggaggaa cacccggatg ggcaaacgca cgaaggtccg ctagggagcg
     1381 acgcgcacga cggcatacgc cggatctcgg tacacgacct cgccgaggtc ggcaaccggt
     1441 tgtgatgctc gaatgacgat gtgggtcacc ccgaagtctc gcgccaggct ccgcgcgcga
     1501 tctccggtca tcttctcgac gccatctcgg atgacgtcga actccatgaa caggcgagcc
     1561 cctccggcct gatcgccata gcgcgtgaga tcgaacggcc tgcagtcaca aatcgcctcc
     1621 acgcgatgcc ggtacgatcg gttgtactcg agatcgaaca ccgactccgg accgtctttc
     1681 aaggtggcga ggacggcgcg gcgcgcgccg taccggaagt ggaggagtga ctcccagtcg
     1741 agcggcagca cgacgagcgc gttggggggg aggtgcgtgc ggcaccaggt ctcgagggcc
     1801 acgtcctctg gagcatccac gaatcggtgc tggatgacgc caggatgggt ggacgtgaag
     1861 gtcggccggg tgtcgccggg gccgaccgcg aagtggaaca tcgcgaccgc ggcgacgagg
     1921 ccaggtccga gccaccacgg cggtggacgc acgtggcagg cgaccctcgt gttgggggtg
     1981 acggtcgccg tacgaacgac tgcgatgagg gcggcgcaca ccacgagcga cgtcaccacg
     2041 atcgcgtggt gcacgagcgg tgggtatgtc caccacggaa cgaggaacgg cagatcgatc
     2101 gcgaatgcaa acgcgaacga gacggacgcc agccgtgctc gccgggtctc ggcgccttgc
     2161 cagcggccca cgacccacgc cgcgcagcac gccgcggcga tgaacacgag gaaccggacc
     2221 gactcccaga ggtggaggaa catcgcttgc gggatcttga gcaagccggt gcccaagccg
     2281 ccagcgccgc agcacaccgc gatcccgagc aggtaaccgc gcgccgcggt cggaaggagg
     2341 cgccgtccga ccgcgaagaa cagcaggcca tacatcacga caaatggccc gaagacccat
     2401 agcgtatcga gcggatagat atggaagtac atgtgctggc gcacgaagtc ccaccattcc
     2461 ggcggcccag gaaacggcac gccgcccgcc ccgcgatggt tcgccagctt cacgatcaac
     2521 ggcgatgccg cgatcagaaa cacgagcgga ccgaggatcg cagttcgcac gcgccggtga
     2581 gatccccgaa cgaccagcgc gctgcaccac agcaagacga ccgcgtgaaa cgcagtcgtg
     2641 ggatgaatcc agaaggtcag ccccgcgacc gcgaagccgg gcagccacgc gccttgcgcg
     2701 ccgagcgcga gggcgtagag caacggcccg ttcgacacgg tccggttcag gacctccccg
     2761 tccatcgacg ggatccccgc cacggtctcg tggctgacga ccacgagcgc agggatgatc
     2821 gcacgggccc agatcccttg atcgcccggg aacaaggcac gtccgagcgc acgcgcgccc
     2881 gcgtacagcg cggcgagcga gacgacgtgg atcgccagat agaggtgttc gatcgagagc
     2941 caagaggcca gctgggcctg gagctcccag aacaggctcg ggtgatgcgc cagcgcatcc
     3001 accgcagggt ctccgaccag gtacgaaggg tcgagcgtcc ggagcaagaa cggcagatga
     3061 actgcatgat cggaaacgcc gaagcgatat ccgttgacga ggagcgacgc gagcacgaac
     3121 acgcccaacc ccacgctttc gtgccacacg gcggtgcaca ccgagcgcag gctcgacacg
     3181 aagcgggcgg accgctcgcg aagtcgcctg tgaagtcgag gctccacgtc attcattcca
     3241 gctacctcgt cgcgcacgcc cgaccgacgc catcagacga cgagggcttc ggcggcggag
     3301 ctccagcgcg atccgcgcca gctccacgcc ggcgcgcacc atcgatgccg gcccgatctt
     3361 cgagccccgc acgtcggccc aggccttgag cggtgcctcg acgatgctgt agcccggatc
     3421 cgccgtccag agccggccga gcagctcgac gtcgaacgcc cagcgagaga tgtacggcgt
     3481 ggcgagcgcc gatcgcagcg catcggtgac ccggaagaac ttggcgccgc actgcgtgtc
     3541 gtagaccgag cgctgcagga cgaccgatgc cgccgccgcg aacacccgac ccaggtagcg
     3601 gcgcacggga tgacgaacga tgtcggcgcc gaggaaggag atccgcgagc cgatcaccgc
     3661 gtcggcgtcg gagtccaggg ccaggctcgc cagccggagc agctcgtcgg ggggcgtcgc
     3721 gaagtccgcg tccgcataac cgacgaccgc agcccgatcg atcgccgcca gcagcccgca
     3781 gcggaccgcc tcggccttgc cgcggttctc tggaagcgcg agcaccgaga catgcggatc
     3841 cgccttgtcg cgcagggcct cgagcacggc gagcgtgcgg tccgtcgagc catcatcgac
     3901 caggatgacg tcgagctggc ggtcgcggac cagcgcagac accgagcgcg gctcgagccg
     3961 gcgctcctcg ttgaagcagg ggatcgcgag cacgaccggg ccccgcgcgc gcgtagcttg
     4021 gggcggtagc gggatctcac ggccaggcag gtcggacaat gacacaaatc acttctccta
     4081 tacagcagac accgcgaacc gcacacacgg agcggccctg actgacgagc ctgtctttca
     4141 cacttcgttt aaagagcgaa tacataccca cccacgacat gtcaaggacc aactcatggc
     4201 tcatcaacag cacgaaccca gatcgcgcga caacaagcca tcagcactac agtaaacgcg
     4261 ttccatcgat ctttcttcgc aggcgaagcc accaacccga gtcccatcac agcttctcca
     4321 gcaggcgaag ccaccagccg actcgcatca cactttccgc aggcaaagcc accagcccga
     4381 ctcccatcac acccccatcc acccccgcct accagcccga ttcgttttgt tcactcccgt
     4441 ccatttcggc tcactcctgt tcactcccgt tcattttata aaatcagttt tcaggtctca
     4501 tgaaatagcg ctggtgtttt caaaccgccc atgcgaagaa gctgttccta ctatcgcgat
     4561 gcagtcgaag tcgaagttcc aatcgcgctc tcaggcactg gacatcgagc tcatccacct
     4621 gatcgaggcg ctcgccgcac agctactctc gtgctctccg gcggacatcg cgcgcgacgt
     4681 gccgtttcgc gagcttggcc tcgactccgc cggcgtgacg atgctcggcg cggagctcgc
     4741 gcgcaccctc gatcgtccga tcccggtgcc ggtgatgtgg acgcacgcca cggtgcgcgc
     4801 gctggccggc tacctcgccg ccccgacgga ccgcaccgcg gccggtcccc tgacgcacgc
     4861 acccgcgaac gcggacgagc cgatcgcact cgtggggctc ggctgccgcg tgccggcgag
     4921 cattgcgagc ccgggcgagc tctgggccgc gctgctcgcc gaggtcgacg cggtccggga
     4981 ggtcccggcc gatcgctggg atctggctcg ctggttctcg gccgaccccg aagcaccggg
     5041 gaccatgacg gcgcgatgtg gcgcgttcct cgacgacgtc gctgcgttcg acgcgggctt
     5101 cttccggatc tcgccccgcg aggcgcgcca gatcgatccg cagcagcgcc tggcgctcga
     5161 ggtcgcggtc gaggcgctcg aggacgcgcg gatcgtgccc tcgaggctcg ccggagggcg
     5221 cacgggcgtg ttcttcggcg cgatgtggca cgactacgcg ctgctcgcgg gcgcggaccc
     5281 ccgcaccatc gagccgcact ccgccaccgg ctgggatacc tcggtcatct cggcgcggat
     5341 cgcgtacgcg ctcgggctcc gcggccccgc gctgacagtc gcgaccgcgt gcagctcgtc
     5401 gctcgtcgcg ctgcacctcg cgacgcagag cctgcggcgc ggcgagaccg atctcgcact
     5461 ggtcggcggc gtcaacctga tgcttcatcc gctgtcgagc gtgctgatgt cgaagttcgg
     5521 cgcgctcggc ccggccgggc gttgccgcgc cttcgccgcg ggcgccgacg gctacgtgcg
     5581 cggcgagggt tgcgtggtcg tcgcggcgcg gcggctatcg gacgccgtcc acgccggcga
     5641 tcggatctac gcgctcatcc ggggcacggc gatcaacaac gacggtgcat cgaacgggat
     5701 cgccgcgccc aacccggtcg cacagatcga cgtactggag gcagcgtggc gcgacgccgg
     5761 catcgcaccc gccgaggtcg gctacgtcga agcccacggc accggcacgc cgctcggcga
     5821 tcccatcgag gccagcgcgc tcgctgcggt gttcgcgcag gaccgccagg cgccgctggc
     5881 gatcggatcg gtcaagacca acttcggcca cctcgaggca gccgcaggcc tgctcggcct
     5941 cgcaaaggcc gcgctctcgc tgttccacgg cgagcttccg cgcagcctgc acttcgacgc
     6001 cccgaacccg cacatcgact tcgacgccca gcgactcgag gtcgtgcagg cgcgccggcc
     6061 gtggcccgag gggcgccggc tcgccggggt cagcagcttc gggttcggtg gcaccaacgc
     6121 gcacgtcgcg ctcgagcagg cgccgttcac gccgcggtcg cgccctcgcc ctcgagcggt
     6181 tgcggccgcc cgcggggacc gcgggctcgt gtggttcttc tcggggcacg gcggccagtg
     6241 gcccgggatg gcgcgcgaac tgatcgcgac cgacgcggtg ttccgcgccg cgatcgagcg
     6301 ctgcgatcac gcgctacacc cggtgacggg ctggtcggtg gtccgcgagc ttgcgggcga
     6361 gccgtcgcag tcgcgcatcg agcagacacg gttcagcgag cccgcgatct tcgcgctcca
     6421 gatcgcgctc gcagaggtgc tcgcgcacgc gggcgtccgt ccggacgcgg tgatcgggca
     6481 gagtatcgga gagatcgccg cagcggtggt gtctggtgcg ctcacgctcg acgacggcgc
     6541 gcgcgttgtg gcccggtggg ccgatctcgt caccgaccac gcctgcggca cgggcggcat
     6601 cctcgtgatc gactgcgccg ccgaggccgc ggccgcgctg atcgaagggc gcgacgggct
     6661 gtcggtcgcc ggcgtcctgt caccgcggca cacctgcctc gcggggtcca ccctcgccct
     6721 cgccgagctc gcgtctgtcg cgcgcggcct ggaccaccgg atcgggcacg tccggatcga
     6781 ctacccgtgc cacggccccg cgatggccgc gaccgcggag cgcctcgccg ccgagctcgg
     6841 cgagctccca gcacaccccg cagcgctgcc gctgtggtcg accgtgacag ccgggccgct
     6901 cgtgggaccg gagatgtcgg ctgcctactg ggcgcgcaac atgcgcgaac cgatgcggat
     6961 cgccgaggcg atggcgcggc tcgtcgcgac ccgcccgatg accgtcgtcg agctcggccc
     7021 acatccgatc atccgcgaag gcctgcaggc gctgttcgag agcatggcgc gcccagactg
     7081 ccgcgcgctg gcgtcgtgcc ggcggggtga gccgggcacg gcgggcctcg cgaccctgat
     7141 gcacgagctg gcgcgagacg ggctgatcga cgcccccacc gccgaacctc ccagcctgat
     7201 cgtggtctcg ggcaagagcc ccgcggcgct gcgcaacaac tgcgctcggc tcgccggcgc
     7261 gatcgagagc gagggcgagg cgctcggcac gatcgccgga acgctcgccg tcgcgcgcga
     7321 tcaccaccca caccgcgtcg ccgtggtggc cgcggatcgc gcgcacgccg ccc
//
LOCUS       DASVOR010001393         4618 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_276484,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001393 DASVOR010000000
VERSION     DASVOR010001393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276484"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1879)
                     /locus_tag="VF516_26470"
     CDS             complement(<1..1879)
                     /locus_tag="VF516_26470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111313.1"
                     /translation="MRIATTLATATALATTTTAQAAAPVSRHPRMLLDPPLRAAWQAQ
                     VRQPHSPVARAIAVCSDARTTPKYDGALYQGSEWAKALQSCLVAWAATGDARHAATSI
                     KFFTALLDDLDQVGDGKGGDRAAWRDSGYAIRMLAPYTALAYDWLYDQLAPELRARAR
                     QRWSSWLTWYREHGYRARAPGNNYQAGYLISATMIAIAEAGEGDPALWSLVANELWGK
                     DMAAALASGGILDGGDWAEGWQYGPLSVAEYALAARVASRAGIRVAGIEPWLAGLLRR
                     HVYALSPGDRVYAGGDTENEAPNLDPALLTLDAVALGDAPGNERRWARGEIARLGLHD
                     DNFVLYDALAAAGDPPADVPRLTWPTWYESAATGTLYARTRWDDRAVWLVTTCQHGLP
                     VDHRHADAGNFVLSRGKDDLIVDPSPYGTQSTLTSNAPTIASARLPRKYSPSQAGWST
                     RTGWDWTAQTRSGVVAARCDYSDQYRFQDRPSDVPEALRDLVLLPSSDGGDASLVVID
                     RAATGSSDRAMYLRFRVPGEIALDGDVATRTIGQSHLTIASVARSTGRPVIGHTKLKD
                     CFNEDYKGRCDAARFPVSDLRLDIDGPEPSAVHVITATAAGADTPRSAPLSGDGWSGV
                     HV"
     gene            complement(1900..2208)
                     /locus_tag="VF516_26475"
     CDS             complement(1900..2208)
                     /locus_tag="VF516_26475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111314.1"
                     /translation="MAAATRTEPCEASRAPFGDVQIQRKPPRGEAGDRAADPMVDDPS
                     APFAAAPADLRFSFAFAAFADRLRGGQVPAHGPPGAVPEWNARFPSNPWSGFTGRARL
                     "
     gene            complement(2270..2779)
                     /locus_tag="VF516_26480"
     CDS             complement(2270..2779)
                     /locus_tag="VF516_26480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111315.1"
                     /translation="MDNADLTLTVLQNIRTDIAALGSRFDGLEHRFDGLEHRFDGLEH
                     RFDGLEHRFDILERRFDTLESRFGTLETRVNGLESEGAATRKDLQACLTREEFRGAML
                     AMNIAQNERFERLEQRMNESDMRSAASHRELQDAIRQLTAQVSQHGSLAARVSLCERD
                     IVDIKRGML"
     gene            3542..>4618
                     /locus_tag="VF516_26485"
     CDS             3542..>4618
                     /locus_tag="VF516_26485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111316.1"
                     /translation="MRWSSWLGVCVLSACASQAPGSGGDCRAAGDCAVPRIVEACLNA
                     ERHDLRATPRLCELAWSLTRNDEAAASGAFYARKTGDQATLKRWVERAPRTLQGARIL
                     HYWGEMLVKRGDLEAAEDTLQRALDLQVNRDPNRATNTALLLLELARSRRPADQSIWL
                     ARIAWQQAELGHNDMMRACAAISLIELLLDLGEFSTASAVIERMDADHSVIWQGTRDS
                     AMARLEAARGRSATAIALFRRASRLDPKKPPTGAPMLPYDAIELVQALLDAGQLAEAR
                     HALDRAAELVGKSPLPSEILTCRLAAVQGSVELAEDRIDAALATVERGLANNCDHAGR
                     VQLLNVQGEVLQRRADARGSAEDAI"
BASE COUNT          637 a         1617 c         1690 g          674 t
ORIGIN      
        1 cgacgtgcac gccggaccag ccgtcgccgg acagcggcgc gctgcgcggg gtgtcagcgc
       61 cggcggcggt cgcggtgatc acgtggaccg cgctcggctc ggggccgtcg atgtcgaggc
      121 ggaggtcgct gaccgggaac cgcgcggcgt cgcaccggcc cttgtagtcc tcgttgaagc
      181 agtccttgag cttcgtgtgt ccgatcaccg gacggcccgt gctgcgcgcg acgctggcga
      241 tcgtcaggtg gctctggccg atcgtgcggg tcgcgacgtc cccatccagc gcgatctccc
      301 ccgggacccg gaaccgcagg tacatcgcgc gatcgctgct gccggtggcg gcgcggtcga
      361 tgaccacgag gctggcgtcg ccgccgtcgc tgctcggcaa caggacgaga tcgcgcagcg
      421 cctcggggac gtcgctgggg cggtcctgga accggtactg atcggagtag tcgcagcgcg
      481 ccgcgaccac gccggagcgg gtctgcgcgg tccagtccca cccggtccgc gtgctccacc
      541 cggcctggct cggcgagtac ttgcgcggca gccgggccga cgcgatggtc ggcgcattgc
      601 tggtcagcgt cgactgcgtg ccgtacggcg acggatcgac gatcagatcg tccttgccgc
      661 gcgacagcac gaagttgccg gcgtcggcgt ggcggtggtc gaccggcagg ccgtgctggc
      721 acgtggtcac cagccacacc gcgcggtcgt cccaccgcgt gcgcgcgtac agcgtgccgg
      781 tcgccgccga ctcgtaccag gtcggccagg tcaggcgcgg cacgtcggcc ggtggatcgc
      841 cggccgcggc gagcgcgtcg tagagcacga agttgtcgtc gtggaggccg agccgggcga
      901 tctcgccgcg cgcccaccgg cgctcgttgc cgggggcgtc gcccagcgcg accgcgtcca
      961 gggtgagcag ggccggatcg aggttggggg cctcgttctc ggtgtcgccg ccagcgtaca
     1021 cgcgatcgcc gggcgacagc gcgtagacat ggcggcgcag caggccggcg agccacggct
     1081 cgatcccggc gacccggatg ccggcccggc tcgccacccg cgccgcgagg gcgtactcgg
     1141 cgaccgacag cgggccgtac tgccagccct cggcccagtc gccgccgtcg aggatgccgc
     1201 cgctcgccag cgccgccgcc atgtccttgc cccacagctc gttggcgacc agcgaccaca
     1261 gcgcgggatc gccctcgccg gcctcggcga tcgcgatcat cgtggccgag atcaggtagc
     1321 cggcctggta gttgttcccg ggggcgcggg cgcggtagcc gtgctcgcgg taccacgtga
     1381 gccacgacga ccagcgctgc cgagcccggg cgcggagctc gggcgcgagc tggtcgtaga
     1441 gccagtcgta ggccagcgcg gtgtacggcg cgagcatccg gatcgcgtag cccgagtcgc
     1501 gccacgcggc gcggtcgccg cccttgccat cgccgacctg atcgaggtcg tcgagcagcg
     1561 cggtgaagaa cttgatcgac gtggcggcgt ggcgggcgtc gccggtcgcg gcccacgcga
     1621 ccaggcagga ctgcagcgcc ttggcccact ccgagccctg gtacagcgcg ccgtcgtact
     1681 tgggggtcgt gcgcgcgtcg ctgcacaccg cgatggcgcg cgcgaccggg ctgtgcggct
     1741 gccggacctg ggcctgccac gcggcgcgca gcggcggatc gagcagcatc cggggatgcc
     1801 tggacaccgg cgcggccgcc tgggcggtcg tggtggtcgc cagggctgtg gcggtggcca
     1861 gggtagtggc gatcctcaca acggcggcga cctgcacacc tacaacctag cgcggccggt
     1921 gaatccggac cacggatttg acgggaatcg agcgttccat tctggcaccg cgccgggcgg
     1981 cccgtgcgcg gggacctgcc cgccgcgcag cctgtcggcg aacgccgcga acgcgaacga
     2041 gaaccgcaga tcagccggag cggcggcgaa cggcgcgctg gggtcatcga ccatcgggtc
     2101 ggcggcccgg tcgcctgcct cgccccgcgg cggcttgcgc tggatctgca catcgccgaa
     2161 cggcgcgcgg cttgcttcgc acggctcggt ccgcgtcgcg gccgccacgc caagcgcgac
     2221 ggctgcccgg gcctcggtga ccgcgcggcg agggccgcag gcccgtcgct cagagcatgc
     2281 ccctcttgat gtcgacgatg tcgcgctcgc agaggctgac gcgcgccgcg agcgacccgt
     2341 gctggctgac ctgggccgtc agctggcgaa tcgcgtcctg cagctcgcga tgcgaggccg
     2401 ccgagcgcat gtcgctctcg ttcatgcgct gctcgaggcg ttcgaagcgc tcgttctgcg
     2461 cgatgttcat tgccagcatc gcgccacgga actcctcgcg cgtgaggcag gcctgcagat
     2521 ccttgcgcgt cgccgcgccc tcactctcga ggccgttgac cctggtctcg agggtgccga
     2581 acctggactc gagggtgtcg aacctgcgct cgaggatgtc gaaccggtgt tcgaggccgt
     2641 cgaaccggtg ttcgaggccg tcgaaccggt gttcgaggcc gtcgaaccgg tgctcgaggc
     2701 cgtcgaaccg gcttccgagc gccgcgatgt cagtccggat gttctgcagg accgtcagtg
     2761 tgagatcggc gttgtccatg ttcgttctcc gagtatagct gtgaatggat ggccgcggac
     2821 cagagcaacg gcagtgccac gcgcaacgtg ccgatctccc acgcgcggct gtccagcttt
     2881 cgtccgaaac ccggacagat ggccgaaatc cggccgctgg ctggacacgc cgagcgcgcg
     2941 cgacagccga taccgcagcg tctttctcgc gtggcggggc atgctcgcaa tggcgcacgg
     3001 cctgcctgcg accacgcagc ggcgctcttc gcccttgggc gtgctgcgaa attttcttag
     3061 ctcgcgcgag tgtcatgcca ccggtcggct gtggctccac cgtctcggcc ctgctggctg
     3121 cgggtcctgt cgcccaggcc gaccgccagc gccggcgcat gcgcaccgag cgctgccgcg
     3181 gctcccgtca gcagggcatg ctggaccgga agatcggctc gcctgcgggc cagtccgttt
     3241 ccgagtcaac ctcgtgacaa tcgatgcgac cgagctcgat gggaatcgag gttccgggaa
     3301 cttgacgccg gcgacgtccg gggatgcgaa cattcccggc tccgccccgt tccggcgctg
     3361 agacccgctc gcgccgcaga gatgcgctcg cctcaggttg aggggatgcg cccatgtgca
     3421 tcccatgtgc ggtcggcgcc gaaatagggg tgaagggccg aggcgcgccg tcgacaatgc
     3481 ccacgatcac cagcctcact atccaattcc atgattgaac aagtttggct atggtgctgc
     3541 catgcggtgg agctcgtggc tgggggtctg cgtcctctcg gcgtgtgctt cgcaggcgcc
     3601 cgggagtggc ggggactgtc gagccgcggg cgattgtgca gtcccgcgca tcgtcgaggc
     3661 ctgcctgaat gcagagcgcc acgatctgcg ggcgaccccc cggctgtgcg agctcgcctg
     3721 gagcttgacc cgcaacgacg aagcggccgc atccggagcg ttctacgccc gcaagacggg
     3781 cgatcaagcc acgctgaagc gctgggtcga gcgcgcgccg cgcacgctgc agggcgcgcg
     3841 gatcctgcat tactggggcg agatgctggt gaagcgcggc gatctggaag ctgccgagga
     3901 cacgctgcag cgggcgctgg acctgcaggt caaccgcgat cccaaccgcg cgaccaacac
     3961 cgcgctgttg ctgctcgagc tggcgcggag tcgccggccc gccgatcaga gcatctggct
     4021 ggcgcggatc gcgtggcaac aggcggagct cggccacaac gacatgatgc gcgcatgcgc
     4081 cgcgatctcc ttgatcgagc tcctgctcga tctcggagag ttctcgaccg cgagcgcggt
     4141 gatcgagcgc atggatgcgg accactccgt catctggcag ggcacgcggg acagcgcgat
     4201 ggcgcggctg gaagcggcga gggggcggag cgcgaccgcg atcgcgctgt tccgcagagc
     4261 gagccggctc gaccccaaga agccacccac cggcgcaccg atgctgccgt acgatgcgat
     4321 cgagctggtc caggccctgc tcgacgccgg ccagctcgcc gaggcccggc acgcgctcga
     4381 tcgcgccgcc gagctcgtcg ggaagtcgcc actcccgtcg gagatcctga cctgccggct
     4441 cgcggcggtc cagggctcgg tcgagctcgc cgaggaccgc atcgacgcgg cgctggcgac
     4501 cgtggagcgc ggcctcgcga acaactgcga ccacgccggg cgcgtgcagc tgctgaacgt
     4561 gcagggcgag gtgctgcagc gccgcgccga cgcgcgcggg agcgccgagg atgccatc
//
LOCUS       DASVOR010001394         2971 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_72555,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001394 DASVOR010000000
VERSION     DASVOR010001394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72555"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VF516_26490"
     CDS             complement(<1..309)
                     /locus_tag="VF516_26490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111317.1"
                     /translation="MSLDEIHRLLAAAPAAQGYLAVATERGDRTYLLGPRTQVDGEVA
                     MLDWRTAPLAEAFFRYRPGEAYEIEAGERTASGRVIARWVIARHGEALIDDTRVIGAD
                     G"
     gene            492..1055
                     /locus_tag="VF516_26495"
     CDS             492..1055
                     /locus_tag="VF516_26495"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8111318.1"
                     /translation="MPDLDDLVWKLGARLPGHDYRVFTTGFVDGVHPRTGAVKRFSLI
                     ACADWVNVLALTADERVVLLRQFRPGVDRVCLEIPGGIIEPGEDPCTAAARELAEETG
                     YTGEHWELIGKVAPNPALQNNTLYTVLARGVALTRPPSPDDGEVLAVETASLADCQRR
                     LVAGEIDHALVVVAFAHLALRRGDLRL"
     gene            1188..1397
                     /locus_tag="VF516_26500"
     CDS             1188..1397
                     /locus_tag="VF516_26500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111319.1"
                     /translation="MSLTQFMEKAEPLKVVINGQEMLAEVKQFSTGSFGWYMNGKTVV
                     SIDGKAVSVQIGMNLTVVGSKDAER"
     gene            1402..2799
                     /gene="asnS"
                     /locus_tag="VF516_26505"
     CDS             1402..2799
                     /gene="asnS"
                     /locus_tag="VF516_26505"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEX8111320.1"
                     /translation="MATSIAAVLAGQQPVGSRITIEGWVRTRRDSKAGLSFLAVHDGS
                     CHDPLQVVAPAELSNYQTDVLRITSGCAVRVTGELVASQGKGQSVELRGESIEVVGWV
                     DDPDHYPMQPKRHTMEYLREVAHLRPRTNVIGAVTRVRHTLAQAVHRFFHERGFFWIH
                     TPIITASDAEGAGQMFRVSTLDAANPPRTPDGKVDFDQDFFGREARLTVSGQLNVECY
                     CLAMSKVYTFGPTFRAENSNTRRHLAEFWMIEPEVAFADLSANAQLAEDFLKAIFRTL
                     LDERKDDMAFFEQRVDPECQKRLAALVESTFTRMEYSEAIRLLEKSGEKFEFPVRWGI
                     DLQSEHERYLTEKVVKGPIAVVNYPKDIKAFYMRLNPDGKTVAAMDVLAPGIGEIIGG
                     SQREERLDMLDARIAEMGLHAPDYGWYRDLRRYGTVPHAGFGLGFERAIQYATGMENI
                     RDVIPFPRAPRQAEF"
BASE COUNT          436 a         1111 c         1011 g          413 t
ORIGIN      
        1 gccgtcggcg ccgatcacgc gggtgtcgtc gatcagcgcc tcgccgtggc gggcgatcac
       61 ccagcgcgcg atcacccggc cgctggcggt gcgctcgccg gcctcgatct cgtaggcctc
      121 gccgggccgg tagcggaaga acgcctcggc cagtggcgcg gtgcgccagt cgagcatcgc
      181 gacctcgccg tcgacctggg tccgcggccc gagcaggtac gtccggtcgc cgcgctcggt
      241 cgcgaccgcg aggtacccct gggccgcggg agcggccgcc agcagacggt ggatctcgtc
      301 gaggctcacg cgagcgatgt tgcgcgcgcg gactgctttg gcaagctagg atcgcagtgc
      361 gacctgcgct aggatcgtcg gcacggtcgg ggattgcccc gtcggcgccc gggccgcccc
      421 gcccggcgag cgccgccgac acgcggccca ggcgatggcc gaagccggcc cggcgcgata
      481 caccgccggc catgcccgat ctcgacgacc tggtgtggaa gctcggcgcg cggctgcccg
      541 gccatgacta ccgggtgttc accaccggct ttgtcgacgg ggtccatccc cggaccggcg
      601 cggtcaagcg attctcgctg atcgcgtgcg ccgactgggt caacgtgctc gcgctgaccg
      661 ccgacgagcg ggtcgtgctg ctccgtcagt tccggcccgg cgtcgaccgg gtgtgcctcg
      721 agatccccgg cggcatcatc gagcccggcg aggacccgtg caccgcggcg gcgcgcgagc
      781 tcgccgagga gaccggctac accggcgagc actgggagct gatcggcaag gtcgcgccca
      841 acccggcgct ccagaacaac acgctctaca ccgtgctcgc gcgcggggtc gcgctgaccc
      901 gaccgccgag ccccgacgac ggcgaggtgc tcgcggtcga gaccgcctcg ctcgccgact
      961 gccagcgccg gctcgtcgcc ggcgagatcg accacgcgct ggtcgtggtg gcgtttgccc
     1021 acctcgcgct ccggcgcggc gacctccggc tttgaccgac ggcccctacc cggcggcgat
     1081 ccggcccacc ggttccgtcc ggccgccaga cgagaccgac cggatgtggc gcagaagccc
     1141 ggtttctggt cagatgcggt cgcatgccgc ccaagacgtc ctgccccgtg tcgctcaccc
     1201 agttcatgga gaaggccgag ccgctgaagg tcgtgatcaa cggccaggag atgctggccg
     1261 aggtcaagca gttctcgacc ggcagcttcg gctggtacat gaacggcaaa accgtggtgt
     1321 ccatcgacgg caaggccgtg tcggtccaga tcggcatgaa cctgaccgtg gtcggcagca
     1381 aggacgccga acgctagcga catggccacg tccatcgccg ccgtgctggc gggccagcag
     1441 cccgtcggta gccggatcac gatcgaaggc tgggtccgca cccgccgcga ctccaaggca
     1501 ggtctgtcgt tcctcgccgt ccacgatggc agctgccacg atccgctgca ggtcgtggcg
     1561 ccggccgagc tgtcgaacta ccagaccgat gtgctgcgca tcaccagcgg ctgcgcggtg
     1621 cgcgtcacgg gcgagctggt cgcgtcgcag ggcaagggcc agtccgtcga gctgcgcggc
     1681 gagtcgatcg aggtcgtcgg ctgggtcgac gaccccgacc actacccgat gcagcccaag
     1741 cgccacacca tggagtacct gcgcgaggtc gcccacctgc ggccgcgcac caacgtgatc
     1801 ggcgcggtca cccgcgtccg ccacaccctc gcccaggcgg tccaccgctt cttccacgag
     1861 cgcgggttct tctggatcca caccccgatc atcacggcgt ccgacgccga gggtgccggc
     1921 cagatgttcc gggtgtcgac gctcgacgcc gccaacccgc cgcgcacccc cgacggcaag
     1981 gtcgacttcg atcaggactt cttcggccgc gaggcccggc tgaccgtgtc gggccagctc
     2041 aacgtcgagt gctactgcct ggcgatgtcc aaggtctaca cgttcggccc gacgttccgc
     2101 gccgagaact ccaacacgcg gcgccacctc gccgagttct ggatgatcga gcccgaggtc
     2161 gcgttcgccg acctctcggc caacgcccag ctcgccgagg acttcctcaa ggcgatcttc
     2221 cgcaccctgc tcgacgagcg caaggacgac atggcgttct tcgagcagcg cgtcgacccg
     2281 gagtgccaga agcggctggc ggcgctggtc gagtcgacct tcacccgcat ggagtacagc
     2341 gaggcgatcc gcctgctcga gaagtccggc gagaagttcg agttcccggt ccgctggggc
     2401 atcgacctgc agagcgagca cgagcgctac ctgaccgaga aggtcgtcaa gggcccgatc
     2461 gccgtcgtca actaccccaa ggacatcaag gcgttctaca tgcggctcaa ccccgacggc
     2521 aagaccgtcg cggcgatgga cgtcctcgcc cccggcatcg gcgagatcat cggcggcagc
     2581 cagcgcgagg agcggctcga catgctcgac gcccggatcg ccgagatggg cctgcacgcg
     2641 cccgactacg ggtggtaccg cgacctgcgc cgctacggca ccgtccccca cgccggcttc
     2701 ggcctgggct tcgagcgcgc gatccagtac gccaccggga tggagaacat ccgcgacgtg
     2761 atcccgttcc cgcgcgcccc gcgccaggcc gagttctagt gcgccgcgcc ggccgccagc
     2821 cgaggtagcc gtggccgact gggtgctcgc cgccgagcgc gcctacgtcg ccgccgcggt
     2881 gggccgtgcc gcccccgccg ccggtgccga gccagcggac gtccccgcgc cgcgcgtgct
     2941 gatgcgctcc gactggcgcg ctgacctccc c
//
LOCUS       DASVOR010001395         1864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_72564,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001395 DASVOR010000000
VERSION     DASVOR010001395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72564"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /locus_tag="VF516_26510"
     CDS             <1..1410
                     /locus_tag="VF516_26510"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX8111321.1"
                     /translation="TDTPRAATPLSVGPVGGTAGGATGEGPGDIVIATRPAPKPVAVG
                     KLIAECAEPIARIDDAEIDGLLERIGDARVVLLGEATHGTSEFYRMRTRITQQLILRH
                     GFAGVAVEADWPDAAAVDRYVRGLPAQPRRWVPFTRFPTWMWRNREVQVLIEWLRAYN
                     QEARRNVSFSGLDLYSMYTSAHEVVHYLDRVDPVAAQAARERYGRLTPWQDDPAAYGR
                     AALIGRMKSCEDEVVAMLRELLARRLDYSAQDGDEFFDAAQNARVVADAERYYRVMYY
                     GSVASWNLRDQHMFDTLKAILDHRGPDTRLVVWEHNSHVGDAAATEMAVRGEHNVGHL
                     CRAAFGDRVYIVGFGTDHGTVAAADNWDEPMQRMRVRPARPDSYERLCHDAGVPAFLL
                     HLRDPVRRELRDELAEPRLERAIGVVYRPATELQSHYFQAVLPLQFDEYIWFDETTAV
                     EPLPVAEPAVAEAEHPFAP"
     gene            1420..>1864
                     /locus_tag="VF516_26515"
     CDS             1420..>1864
                     /locus_tag="VF516_26515"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8111322.1"
                     /translation="MRAIRAMVLERVGAPLVLRELAMPVPGPGEIQLAVEACGVCRTD
                     LHVVDGELPDPRLPLVPGHQIVGTVSALGPGVTGLSLGDRVGVPWLGWTCGRCLYCTT
                     GRENLCRSARFTGYHRDGGYAEAAVADARYVFPLPPGYTALEAAPL"
BASE COUNT          243 a          707 c          661 g          253 t
ORIGIN      
        1 accgacaccc cgcgcgccgc gaccccgctc agcgtcggtc cggtcggcgg aaccgccggc
       61 ggtgcgaccg gcgagggccc cggcgacatc gtgatcgcga cgcgccccgc gccgaagccg
      121 gtggccgtcg gcaagctgat cgccgagtgc gccgagccga tcgcccggat cgacgacgcc
      181 gagatcgacg gcctgctcga gcggatcggc gacgcccgcg tcgtgctgct cggcgaggcg
      241 acccatggca cctcggagtt ctaccggatg cgcacgcgga tcacccagca gctgatcctc
      301 cgccatggct tcgccggcgt cgcggtcgag gcggactggc ccgacgccgc cgcggtcgac
      361 cgctacgtcc gcggcctgcc cgcgcagccc cggcgctggg tgccgttcac gcggttcccg
      421 acctggatgt ggcgcaaccg cgaggtccag gtgctgatcg agtggctgcg cgcctacaac
      481 caggaggccc ggcgcaacgt cagcttctcc ggcctcgacc tgtacagcat gtacacctcg
      541 gcccacgagg tcgtgcacta cctcgaccgg gtcgatccgg tggccgcgca ggccgcgcgc
      601 gagcgctacg gccgcctcac tccctggcag gacgatccgg ccgcgtatgg ccgcgccgcg
      661 ctgatcggcc gcatgaagtc gtgcgaggac gaggtggtcg cgatgctgcg cgagctgctc
      721 gcccgccggc tcgactactc ggcccaggac ggcgacgagt tcttcgatgc cgcgcagaac
      781 gcccgggtgg tcgccgacgc cgagcgctac taccgcgtga tgtactacgg ctcggtcgcg
      841 tcgtggaacc tgcgcgatca gcacatgttc gacaccctga aggcgatcct cgatcaccgc
      901 ggtccggaca cccggctcgt ggtgtgggag cacaactcgc acgtcggcga cgccgcggcg
      961 accgagatgg cggtgcgcgg cgagcacaac gtcggccacc tgtgccgcgc ggcgttcggc
     1021 gaccgggtgt acatcgtcgg gttcggcacc gatcacggca cggtcgccgc cgccgacaac
     1081 tgggacgagc cgatgcagcg catgcgggtc cggccggcgc gccccgacag ctacgagcgg
     1141 ctgtgccacg acgccggcgt cccggcgttc ctgctccacc tccgcgatcc ggtgcgccgc
     1201 gagctgcgcg acgagctcgc cgaaccgcgg ctcgagcgcg cgatcggcgt ggtctaccgg
     1261 cccgccaccg agctgcagag ccactacttc caggcggtcc tgccgctgca gttcgacgag
     1321 tacatctggt tcgacgagac caccgccgtc gagccgctgc cggtcgccga gccggcggtg
     1381 gccgaagccg agcacccgtt cgcgccgtag agtacggcca tgcgcgcgat ccgtgcaatg
     1441 gtgctcgaac gcgtcggtgc gccgctcgtc ctccgcgagc tggcgatgcc ggtcccggga
     1501 cccggcgaga tccagctcgc cgtcgaggcc tgcggcgtgt gccggaccga cctccacgtc
     1561 gtcgacggcg agctgccgga tcccaggctg ccgctggtgc ccggccacca gatcgtcggc
     1621 acggtgtcgg cgctcggccc cggcgtcacc gggctgtcgc tcggcgaccg ggtcggcgtg
     1681 ccgtggctgg gctggacctg cggccgctgc ctctactgca cgaccggacg cgagaacctg
     1741 tgccggtcgg cgcggttcac cggctaccac cgcgacggcg gctacgccga ggccgcggtc
     1801 gccgatgcgc gctacgtgtt cccgctgccg ccgggctaca ccgcgctcga ggccgcgccg
     1861 ctgc
//
LOCUS       DASVOR010001396         2000 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_15099,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001396 DASVOR010000000
VERSION     DASVOR010001396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15099"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..>2000
                     /locus_tag="VF516_26520"
     CDS             132..>2000
                     /locus_tag="VF516_26520"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8111323.1"
                     /translation="MSLAESGIVPAWIDTAVDPCTDFYAFACGGFMKTAVIPPDRSSW
                     GAIELVVKDSEDVLHQVLDAAARAPSGDPTLDKIGAYYASCMDEAAIERAGTAPIQPL
                     LDAIARVTDARSAAAAVIAMHAARVFPLFAIGPQQDFGDATQVIASIDQAGLGLPDRK
                     YYLESKGNMAKTREIYVAHLGRLFGLLGQPAKVARAAAADAMRIETQLARLQQDEVVR
                     RDPHAVYHRVDRAGLEGKIAPSFPWGDYLAALGVPGVTAITVNDPAYYTAITRLLQTE
                     KPAALRNYLTEVVLRRSADNLGKAWVDEAFTMRKALAGVKELPPRWRRCLRNVDRDLG
                     ELLGQSYVKARFAGDAKVRAVDLTRSVLAAMRVELDQLPWMDGTTRAAARQKLDRMAY
                     LVGFPDKWKVYDFEVKRTDFAGNVRAAGHWELARELAKIGKPVDRFDWGMTPPTVNAY
                     YDPTLNEIALPAGQLQPPFFGATFHPAVNFGSTGGGTIGHEMTHGFDDEGSQFDADGN
                     LREWWSKPTREKFTAATQCVVDQYAQYEAVPGVKLNGKLTAGENIADIGGVKLAYQAY
                     QTWKAAQKPPPPAKVDRYTDDQLYFLAYAQSWCDKQTPEVLETRAHSNPHSPPMWRV"
BASE COUNT          308 a          771 c          672 g          249 t
ORIGIN      
        1 gctccggccc gcggtcgtct ccgaccacgc cgtcgcacgt cgcggcgcct cccgcagcag
       61 cgcccgccgg ggacgctgcc cccgcggcgc ccagccgtgg cgacccgtcg cacgtcgcgt
      121 gggcgccgca gatgtcgctc gccgagtccg gcatcgtccc ggcgtggatc gacaccgcgg
      181 tcgacccgtg caccgacttc tacgcgttcg cgtgcggcgg gttcatgaag acggcggtca
      241 tcccgcccga ccgctcgtcg tggggcgcga tcgagctcgt ggtcaaggac tccgaggacg
      301 tgctgcacca ggtgctcgac gccgcggcca gggcgccgtc cggcgacccg acgctcgaca
      361 agatcggcgc ctactacgcg tcgtgcatgg acgaggcggc gatcgagcgc gccggcaccg
      421 cgccgatcca gccgctgctc gacgcgatcg cccgggtcac cgacgcgcgg tcggccgccg
      481 ccgcggtgat cgcgatgcac gccgcccgtg tgttcccgct gttcgcgatc ggcccgcagc
      541 aggactttgg cgacgcgacc caggtgatcg cgagcatcga ccaggccggc ctcggcctgc
      601 ccgaccgcaa gtactacctc gagagcaagg gcaacatggc gaagacccgc gagatctacg
      661 tcgcccacct cggccggctg ttcgggctgc tcggccagcc cgcgaaggtc gcccgggccg
      721 ccgccgcgga cgcgatgcgg atcgagaccc agctcgcccg cctgcagcag gacgaggtcg
      781 tgcgccgcga cccccacgcg gtctaccacc gcgtcgaccg cgccgggctc gagggcaaga
      841 tcgcgccgag cttcccgtgg ggcgactacc tcgcggcgct cggcgtcccc ggcgtcaccg
      901 cgatcacggt caacgatccg gcgtactaca ccgcgatcac caggctgctc cagaccgaga
      961 agccggcggc gctgcgcaac tacctcaccg aggtcgtgct gcgccggtcg gccgacaacc
     1021 tcggcaaggc gtgggtcgac gaggcgttca cgatgcgcaa ggcgctggcc ggcgtcaagg
     1081 agctgccgcc gcgctggcgg cgctgcctgc gcaacgtcga ccgcgatctc ggcgagctgc
     1141 tcggccagtc gtacgtcaag gcgcggttcg ccggcgacgc caaggtccgc gcggtcgacc
     1201 tgacccgctc ggtgctcgcc gcgatgcggg tcgagctcga ccagctgccg tggatggacg
     1261 gcacgacccg cgccgccgcc aggcagaagc tcgaccggat ggcctacctg gtcggcttcc
     1321 ccgacaagtg gaaggtctac gacttcgagg tcaagcgcac cgacttcgcc ggcaacgtcc
     1381 gcgccgccgg gcactgggag ctcgcccgcg agctcgccaa gatcggcaag ccggtcgacc
     1441 gcttcgactg gggcatgacg ccgccgaccg tcaacgccta ctacgacccg acgctcaacg
     1501 agatcgcgct gcccgccggc cagctccagc cgccgttctt cggcgcgacc ttccaccccg
     1561 cggtcaactt cggctcgacc ggcggcggca cgatcggcca cgagatgacc cacgggttcg
     1621 acgacgaggg cagccagttc gacgccgacg gcaacctccg cgagtggtgg agcaagccga
     1681 cccgagagaa gttcaccgcg gcgacccagt gcgtggtcga ccagtacgcg cagtacgagg
     1741 cggtccccgg cgtcaagctc aacggcaagc tcaccgccgg cgagaacatc gccgacatcg
     1801 gcggggtcaa gctcgcctac caggcctacc agacctggaa ggccgcccag aagccgccgc
     1861 cgcccgccaa ggtcgaccgc tacaccgacg atcagctgta cttcctggcc tacgcccagt
     1921 cgtggtgcga caagcagacg cccgaggtcc tcgagacccg cgcgcactcc aacccgcact
     1981 cgccgccgat gtggcgggtc
//
LOCUS       DASVOR010001397         2609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_248297,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001397 DASVOR010000000
VERSION     DASVOR010001397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248297"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1061)
                     /locus_tag="VF516_26525"
     CDS             complement(<1..1061)
                     /locus_tag="VF516_26525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111324.1"
                     /translation="MQLRFLRHKVGAGVLALAGTWTPAAVAGGDPGAASGAVGLDHPI
                     LVLESYVGQRPANADAVLAPLLDELEQRGFATRPASVLELVGGGAPRPGVLDRDTTAA
                     EITQSAESGYEAYTRGRFAEAEAALKRVIERIHRNPALLVLDTNNLDATFKILVALAL
                     SQAKRGDTGGSVATMLELIRTFRHRPISRVHYGPDAEQFYRAVARQAQALGRGELSIS
                     VTSEQAVIFVDGQIRGLGKAALSDLIPGVYRVFIQAPATPGRQYEIEVTAGRHTVLRV
                     DWELDSALWLTDSWVGFMYATEAERARQAGFAGTLARRWAGGTPLAVVGMAQLSGKPA
                     ISGQLVDATGRVVRGAAVLL"
     gene            complement(1061..2194)
                     /locus_tag="VF516_26530"
     CDS             complement(1061..2194)
                     /locus_tag="VF516_26530"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8111325.1"
                     /translation="MPGKVGACGGATPVCGIDEACHPCTMDTDCASQICLPDGSCASP
                     IDVLYVSPTGSDLATCMPDDHCSLARALALIDGTKSTIRLDPAHYNLLATVVLPHDLH
                     LVGRGATLDRNAADSGAVLSIAAGTHISLDYVTVTGGDGSSGDGIACTNATLTLREVT
                     IQGNAGSAISGSGCQLVVSHAQLLNNQAAGIVTSMPSDVVLVRSLVSGNASVGLALAM
                     TTYDIENNVIVKNGGPNGLGGVFITQILGGTVGRRVFGFNTVSQNSGPFGNVSGVLCD
                     QSVGVPLAMTSNIVVNNAGPAQISGGACVWTYSDIAPGPVSGTGNRSSDPLFVDPVHN
                     NFHLQLASPARDAADPAATLAVDIDGDARPQGAGRDMGADEIK"
BASE COUNT          385 a          899 c          958 g          367 t
ORIGIN      
        1 agcaggacgg cggcgccgcg cacgacccgg ccggtcgcgt ccaccagctg gccgctgatc
       61 gccggcttgc ccgacagctg ggccatcccg acgacggcga gcggggtccc gccggcccag
      121 cgccgcgcga gcgtaccggc gaacccggcc tggcgggcgc gctcggcctc ggtggcgtac
      181 atgaagccga cccagctgtc ggtcagccac agcgccgagt cgagctccca gtcgacgcgc
      241 agcaccgtgt ggcggcccgc cgtcacctcg atctcgtact gccggcccgg ggtcgccggg
      301 gcctggatga acacgcggta cacgcccggg atcaggtcgg acagcgcggc cttgcccagc
      361 ccgcggatct gaccgtcgac gaagatcacc gcctgctcgc tggtcacgct gatcgacagc
      421 tcgccgcggc cgagcgcctg ggcctggcgg gcgaccgcgc gatagaactg ctcggcgtcg
      481 ggcccgtagt ggacccgcga gatcggccgg tgccggaacg tccggatcag ctcgagcatg
      541 gtcgcgacgc tgccgccggt gtcgccgcgc ttggcctggc tgagcgcgag cgcgacgagg
      601 atcttgaatg tcgcgtccag gttgttggta tcgagcacga ggagggccgg gttgcggtgg
      661 atccgctcga tcacgcgctt gagcgcggcc tcggcctcgg cgaaccggcc gcgggtgtaa
      721 gcctcgtagc cggactcggc cgactgggtg atctctgcgg cggtggtgtc gcggtcgagc
      781 acgccgggcc gcggcgcgcc gccaccgacc agctccagga ccgacgccgg ccgggtggcg
      841 aacccgcgct gctcgagctc gtcgagcagc ggcgccagga ccgcatcggc gttcgccggg
      901 cgctggccga cgtacgactc gagcacgagg atcgggtgat cgaggcccac ggcgccgctc
      961 gcggcgccag gatccccgcc ggcgacggca gccggcgtcc acgtgcctgc gagcgcgagc
     1021 acgcccgcgc cgaccttgtg ccggagaaat cgcagctgca tcacttgatc tcgtcggccc
     1081 ccatgtcgcg accggcgccc tgcggccggg catcgccgtc gatatcgacc gcgagcgtcg
     1141 ccgcgggatc ggcagcgtcc cgcgccggcg acgcgagctg gaggtgaaag ttgttgtgga
     1201 ccggatcgac gaacagcgga tcgctggacc ggttgccggt ccccgacacc ggcccgggag
     1261 cgatgtcgga gtacgtccag acgcaggcgc cgccgctgat ctgggccggg cccgcgttgt
     1321 tgacgacgat gttgctggtc atcgcgagcg gcaccccgac gctctggtcg cacagcacgc
     1381 cggagacgtt gccgaacggg cccgagttct gggacaccgt gttgaacccg aacacgcgac
     1441 gccccacggt gccaccgagg atctgggtaa tgaagacccc gcccaggccg ttcggcccgc
     1501 cgttcttcac gatgacgttg ttctcgatgt cgtaggtcgt catcgcgagg gcgaggccga
     1561 cgctcgcgtt ccccgagacc agcgagcgca ccagtacgac gtcgctcggc atcgaagtga
     1621 cgatcccggc ggcctggttg ttgagcagct gggcgtgcga gaccaccagc tggcaccccg
     1681 agccgctgat cgcgctgccg gcgttgccct ggatcgtgac ctcgcgcagg gtcagcgtgg
     1741 cgttggtgca tgcgatgccg tcgccggatg agccatcgcc gcccgtcacg gtgacgtagt
     1801 cgagcgagat gtgcgtcccg gcggcgatgc tgagcaccgc gccggagtcg gcggcgttgc
     1861 gatcgagggt ggcaccgcgg ccgaccaggt gcagatcgtg gggcagcacg accgtggcca
     1921 gcaaattgta atgcgcgggg tcgagccgga tcgtcgactt ggtgccgtcg atcagcgcga
     1981 gcgcgcgggc cagcgagcag tggtcgtcgg gcatgcaggt cgccagatcg ctgccggtcg
     2041 ggctcacgta gagcacgtcg atcggcgatg cgcacgatcc gtcgggcagg cagatctgcg
     2101 acgcgcagtc ggtgtccatc gtgcacgggt ggcaggcctc gtcgatgccg cacaccggcg
     2161 tcgcgccgcc gcacgcaccg accttgcccg gcatgcacgg aacacacatc gagcgggtta
     2221 tatcacagac cggtgtcgag ttcgtgcatt gctcgtcttg cgtgcagagt cctgcgtcga
     2281 tccccgcgcc gatcccggtg cgaccgatgc aatcgaggtc ctcgggatgg gtcgcgcaga
     2341 actcgggatt gagctgccgg cacgccgcca gggcgagcgc gagccccgcg ggactgaaaa
     2401 acgagggtga gcggaccgag cgtgagcgga ctgtcgacat ggtgtctcct gtgcaatgcc
     2461 cgtgggatcg agagccggac gccggtgcag cccgggtccg ccaaggttgg aatccagaga
     2521 tggaacgggt cagaacgcgg tggcccaccc cgcgacgagc ccgcgaccgg cgatcgcgac
     2581 gcccggcgcg gcgggtcccg gcatccgcc
//
LOCUS       DASVOR010001398        17367 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_511009,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001398 DASVOR010000000
VERSION     DASVOR010001398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17367
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511009"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..392
                     /locus_tag="VF516_26535"
     CDS             <1..392
                     /locus_tag="VF516_26535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111326.1"
                     /translation="PAGAPDPYASILADAPPGATVAVWVGQPERLDYARHRVIDLRAP
                     AAARLRVHRFAPHTSQLAALVSGVAASFLLIEDDDVRIERIHDDVVYRLACRQPRPMC
                     DDDLEALAAQHPLVARRGNLRLIDLGR"
     gene            482..1300
                     /locus_tag="VF516_26540"
     CDS             482..1300
                     /locus_tag="VF516_26540"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8111327.1"
                     /translation="MFDKLNHHFVRCRGCGLERISPQPTDETLARIYGAHYYDAWGLH
                     DNEDTVARLKKRTFQYVLGKLPRPAPPARLLDCGAATGFLLEVAREVGHDPYGLELSE
                     FGASAIAGKFGADHVFCGELPHAKFAGVEPGGFQVVTMCDYIEHVRDPRQTLALARRW
                     LAPGGALAITTPDAGSPSRRLLGTGWTHYKIEHLYYFNKRNLERLLQEVGFTRVRFYP
                     LWKSLTLDYIGHQFEVYPHRALTPFARALRRMPRALRALPLPFTTGELLATAHA"
     gene            1293..2267
                     /locus_tag="VF516_26545"
     CDS             1293..2267
                     /locus_tag="VF516_26545"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEX8111328.1"
                     /translation="MLERWWWRWGWVVRWSGTALGVLYVARLIHPGKLAAAFSVVSLG
                     AVLGSIAIVAAGIELGAVRWRIALRAYGARSQPGLLTASRLYFIAVFYNTFLPGAVAG
                     DVVRGVATRDSFGEHGATGALAVVFVERLLGLFGVFALILAGIWLVGDALGDRSMWWG
                     SLAGGAASLAALFLLPLGRRLGRFLPAPLARIAERLPVVVRPRDFAAAALLSLATQLA
                     AVIAGWLVLRDLHPRITFGDALLILPVAAATAFLPITVGGTGAREAAFVFLCGRVLGM
                     ASDDAVAASLLIWLATLVIGAFGGLLALTGRGASDDQSKLRTASITRP"
     gene            complement(2228..3343)
                     /gene="rffA"
                     /locus_tag="VF516_26550"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(2228..3343)
                     /gene="rffA"
                     /locus_tag="VF516_26550"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HEX8111329.1"
                     /translation="MIPFNRPAAIGLERIQEAWATGRLAGDGAFGRRAEQLLQAAVGV
                     RRALVTTSCTHALEIAALLLDLGPGDEVILPSFTFVSTANAFVLRGARPVFADVRPDT
                     LNLDEARVAELVTPRTRAIVPVHYAGVGCEMDALGEIARASGAVVIEDNAHGLFGAYR
                     GRPLGALGALATQSFHETKNFSCGEGGALLINDERFAERAEILREKGTDRSKFFRGQV
                     DKYTWVDVGSSYVLSELLGALLVAQLEARDQIQRRRAQIWQRYASELAGWAGAHDVRL
                     PVVPAHCEQAFHMFYLRVPGARRPALLDHLRARGVQAVFHYVPLHLSPMGLRHGGRPG
                     MCPVTEVIADELVRLPFYYDLDDADQGRVIDAVRSFD"
     gene            complement(3340..4050)
                     /locus_tag="VF516_26555"
     CDS             complement(3340..4050)
                     /locus_tag="VF516_26555"
                     /inference="COORDINATES: protein motif:HMM:NF020463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HEX8111330.1"
                     /translation="MTERTVGVILQPSYIPWRGYFDLIHRADVFVFYDDVQYDKHGWR
                     NRNRIKTPSGTQWLTIPVASKGNVTTGLALADARIVWTRDWAKKHALTLRGCYGKAPY
                     FADHAAMIEGFYAGRPERLVDFTIETTLALARALGITTTRFVRSSELGVSGTRTERLI
                     RILDKVSANHYISGPSARAYLDEALFAEAGIGLEYMVYDYPDYEQLYPPYDPQVSVID
                     LMVMTGPRAPDHIWGRAP"
     gene            complement(4058..4930)
                     /gene="rfbA"
                     /locus_tag="VF516_26560"
     CDS             complement(4058..4930)
                     /gene="rfbA"
                     /locus_tag="VF516_26560"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEX8111331.1"
                     /translation="MKGIVLAGGAGTRLYPMTRAVSKQLLAVYDKPMIYYPLSTLMLG
                     GIREILLICAPRDREAFEATLGDGRNFGIRIDYAVQPAPRGIAEAFLIGADFIGSDPV
                     ALVLGDNLFYGMGLTERFETAARLTSGALIFGYYVKDPSQYGVVEFTPTGEVISLEEK
                     PRQPRSHYAVPGLYFYDNEVVEIARRLRPSARGELEITDVNRVYLEAGTLRLIVLGRG
                     TAWLDTGSPDALLQASNFIQTVETRQGLKIGCLEEIAYRRGFIDRDTLHAVGRALDKT
                     EYGQYLLALAAEPR"
     gene            complement(4993..6600)
                     /locus_tag="VF516_26565"
     CDS             complement(4993..6600)
                     /locus_tag="VF516_26565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111332.1"
                     /translation="MICVAAIAVLCWRMLHGIDFTDEAFYIALPLRFALGDRPFVDEL
                     NIAQTAGLLLYPFIKAYLWIAGGTTGIVLFIRALYVVVFGLVGWSAYALARRCIPRPA
                     ALLCGAMCMLVLPHGIPGLSYNTLSMALMTSGLFVIARALLFGEPPRRWWRHPLLWAG
                     FAHGAATFAYPSQLVTTAATAIAVCALARGQRVRAVAMYAAGGLAFVALISPLFVRAG
                     LRNLRIELAYQAGAGDAFSLPVAWARFQELLELHPQLPRALIAIVAVIVIARWLPLLA
                     ACGAVAIPLLARGSDQGGHLASMAYLTCFALFGPVLAAVIRERRVARVLEFAVIVPAM
                     VSGAVICLSSSNRAYAAGIGLYPAAITTAILLVMFIVQTAARSGWRALHPYLALAPAA
                     AMWALLACLVSDGGYYRDGPGSELTERVTSGPYRGLYTTPAKAEILRDLTSDIESYVQ
                     GPRALFLYDLPAGYLIAMRRPLVSSVWTFAIGTRHDQDVRLFKKWAKPGELVILDTPP
                     WVTLETMAAERSDLLVIRPWYRVYRIR"
     gene            6984..8024
                     /locus_tag="VF516_26570"
     CDS             6984..8024
                     /locus_tag="VF516_26570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8111333.1"
                     /translation="MKYSFVVPIYNDGALARDFCREVEIAFREYLGHGDLARDLEVIF
                     VDDGSTNDSPQLLKQVCDEFRFARLILLARNFGQHIAVTAGYQAARGDYVGMLNVDQE
                     DPPSGLPLLLDTLRDSEYDIVGGRYQRRWVRLTTRITSYLFHTLMNRLTGYRTPVNAS
                     TMRVMTRRAVDLYNTLTEKSRYLPGLERWLGLRYGWVQVVHQPRRVGKSSYNFRRRLR
                     MAIATIISFSDFPLRLAVKFGLLVAASGVLLGTAIIVDKLFFRALMPGYTSTIATIVF
                     LGGVQIAVTGVASLYIGRILSEVQGRPLFIVRETYGGAVRELEMPAPSPPGAVLRTTS
                     VDAGSRATGDSS"
     gene            8086..8340
                     /locus_tag="VF516_26575"
     CDS             8086..8340
                     /locus_tag="VF516_26575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111334.1"
                     /translation="MSVGVFALCLALLLVRSYHPLYDAPATNGDETCSLAQAMNSLRL
                     GSPGYGWTAQLKPWTAGRFNQPVYLVVYGTLMLYVHRCGG"
     gene            8662..8970
                     /locus_tag="VF516_26580"
     CDS             8662..8970
                     /locus_tag="VF516_26580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111335.1"
                     /translation="MARALGIVIATSLAVFVLVNFVLQHTPHSIPNVAFAAELTNVVS
                     RATHPWMVKDLISLLRNFPGVLPTAGFPPPARSCSCVAMSGHSRSSWCAWRSCCSSPG
                     "
     gene            9276..10133
                     /locus_tag="VF516_26585"
     CDS             9276..10133
                     /locus_tag="VF516_26585"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF018696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8111336.1"
                     /translation="MSDRYQDVIQANIAVHSKVAAVYSTTEPHFRPENLAVVESVLKR
                     LVAETRATSLLDLGCGTGFMINLAKRHVARIVGIDVTRAMLDQVDTSGPARIELHEHD
                     TGSFHVEPGSFDLVTAYSFLHHLYDIRPTLATAARALRKGGRFYVDLEPNFHFWEAIS
                     RLPTGAGAPSYDPLVTREIDAVVHKDDQMNETFGIPNTVFNDAEYGKNIAGGFQEDAL
                     RDALREVGFSDVTFTYYWYLGQALLVNDKSKSRDQCLADAALVDALLQKVMPLSRHLY
                     KYIGFVAIR"
     gene            10147..10989
                     /locus_tag="VF516_26590"
     CDS             10147..10989
                     /locus_tag="VF516_26590"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8111337.1"
                     /translation="MRVLIIGNRSFIALRLRERLGKEHEVLLAGRTGDADVQFDLTAT
                     GAPPREVPRCDAIIHCAASFEPNTPDGILRNELVNGVGALRVAELATRIACGRIVHLS
                     TVSVFNDDHGRLRDSYALSKRHGQDNLQLVCDAAGIELCTLCPTAIYDERGAGRHHQP
                     LLYRIVDCARRGEDFPLYGSADPARNYLHVRDLAELVAGVMRAGVSGTFPVVFPRDYR
                     ITEIAELAFAVFGTTGRIVRRPDMPDIPVVRYPRSRELFDRLGWVPALDLEAGFRLLR
                     DHHG"
     gene            10982..11977
                     /locus_tag="VF516_26595"
     CDS             10982..11977
                     /locus_tag="VF516_26595"
                     /inference="COORDINATES: protein motif:HMM:NF009402.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8111338.1"
                     /translation="MAEPAPARRRHVLVTGVGAIIGYGVVHALRRASYPVQIIGMDIY
                     GHAVGQTWCDAFERAIPVADPGYIAFLRDAIARHAIDLVIPAIEQDVTFLSRHRAEFA
                     ATGARLALNAASLIEQSDDKWRMHQALQALGCPTIDTMLAGEFDALAAHLGLPFLVKP
                     RRSYASKGLARIHDAEDLAYHRRKLGDNFMAQRIVGDADSEYTSSSFGYGDGTGSRKI
                     VFQRWLAQDGSTARAQVVDEPSLEHEMDRLTRAFRPLGPTNFQFRRHEGRFYLLEINA
                     RISSSTSLKRAFGMNEAEMCIAHYLDGERPDFGSVRKGTAVRYIEDMISYDKPTF"
     gene            11994..12662
                     /locus_tag="VF516_26600"
     CDS             11994..12662
                     /locus_tag="VF516_26600"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase domain-containing protein"
                     /protein_id="HEX8111339.1"
                     /translation="MSLRIVGVADTHLFHDRLAVPDGDVFVHAGDLCRGGSLPELAQA
                     AAWIRALPHATKIVVAGNHDWPFVRDRGRAIELLGPDVIYLEDSGLTIGGLSFWGSPW
                     QPIYGAWAFNLERGAALAARWALIPDRVDVLITHGPPQGIGDQGPISSRAGCEELRAR
                     VEVVRPLLHLFGHIHQDGGLWQHDGVVFANVTTADCRREPTVIDVAAEDRRVHPVEVP
                     PPAG"
     gene            complement(12665..13453)
                     /locus_tag="VF516_26605"
     CDS             complement(12665..13453)
                     /locus_tag="VF516_26605"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8111340.1"
                     /translation="MTARPRDEAILEVLARDGYAIVPGVLTADRVAELRRDLEAAIAA
                     EVAYHRGKDYVDYGMVLVCCTHARSFVEVLADRGVMSIIEHILGEGCIVYAYTSSSMP
                     PGASNYSARIHVDCPRLIPGYATNAGVMLLLDEFTAENGATWVLPGSQHTREPPTAEY
                     FFANARRVIAPAGSAVYLDPRVWHAGGQNHTAAWRHSITLNMCRPYMKQRLDIPKMMA
                     ASGVDLTGVSDRALQKMGFYAQVPESLEEYYLPPEQRKFRQKTE"
     gene            13533..14651
                     /gene="rfbB"
                     /locus_tag="VF516_26610"
     CDS             13533..14651
                     /gene="rfbB"
                     /locus_tag="VF516_26610"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: protein motif:HMM:TIGR01181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEX8111341.1"
                     /translation="MAGFEPKRFLITGGAGFIGCNLTRALLADAAARGVARIVVLDAL
                     TYAGHRVNLDGVLGDPRLEFVEGDICDRALVERLFAAHAIDAVLHLAAESHVDRSIEA
                     PDAFIRTNVTGTFTLLDVARRAWAGRCDATRFVHVSTDEVYGALGETGAFTETTPYAP
                     NSPYAASKAASDLLARSYFKTYGLPVVTTNCCNNYGPYQLPEKLIPLMIANACEGKPL
                     PVYGAGKQIREWLHVEDHCAGLWLAMTRGTPGESYNFGSGDEMTNLQLVELLADLIDG
                     QLQRARGTTRALIRFVPDRQGHDFRYAIDCSKARRALGWQTSRTLQVQLAETVRWYLA
                     NPGWRAAVASEEHHRFLSRQYRDQRGGPDPAGTSTGTD"
     gene            complement(14676..15521)
                     /locus_tag="VF516_26615"
     CDS             complement(14676..15521)
                     /locus_tag="VF516_26615"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8111342.1"
                     /translation="MDGAGMDLGRIGHGLALGSDGIWRARTAAPALSFPADGHDVCLG
                     VESDSFWFAHRNACIVAALARAGVAGPLLDVGGGNGAVSQALERAHIETVVLEPGDAG
                     ARNARARGLRSVVCATLEDAGFEAGSFGAAGLFDVIEHVADDEGLVREVHRVLRPDGA
                     LGVTVPAYRWLWSEEDELAGHYRRYTLDELVATLSRCGFEVAYRTYFFAPLVAPVFVL
                     RSVRGWLGRRSDAAVERGAARQHVPSGLVRRAIDGLLAPELRRIRAGRSVPFGTSCLV
                     VAVRR"
     gene            complement(15530..16102)
                     /locus_tag="VF516_26620"
     CDS             complement(15530..16102)
                     /locus_tag="VF516_26620"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8111343.1"
                     /translation="MVAPSAVIGDRTQIWLHCQIRENTRIGEDCILGKNIYIETGVHI
                     GNRCKIQNNVSVFAGVTVEDGVFLGPHVCFTNDLVPRAINPDGSLKSAADWHITPTRV
                     CTGAAIGANSTIRCGVTIGAWAMIGAGSVVTRSVPPYALVYGNPARQHGWVTPAGERA
                     TFDAGGRFVDHTGFALRLIRDAAGERVEPA"
     gene            complement(16196..17302)
                     /locus_tag="VF516_26625"
     CDS             complement(16196..17302)
                     /locus_tag="VF516_26625"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8111344.1"
                     /translation="MIPFIDLHPVSRLVADAVAPRWAGVVERCEFVGGAGVQQLEAEI
                     ARFCATRAAVACSNGSDALLLALQAAGVRPGMHVALPNLTFWATFEAVVQLGAIPVLI
                     DISADDLQLDLGELQRAHARYGFRHAIIVHLYGWASAALSEIRTFCAGHDICLIEDAA
                     QAIGVELDGRPLLAGAALATLSFYPAKVIGGCMDGGAVVTGDEALAAAVRSLANHGRS
                     AHYSYQQVGWNARMGGLAAHYLLEMMTHAGEILAARRRLVAAYRAQWTGIAGRLRGPA
                     SAIAENGYLLVVECERPADDCVGAFEQRAIGVARTYPETIDQQAPARGRFVAVGELAR
                     SRAFVRRVLNLPLYFGLPEPAVAEVVGAAREILR"
BASE COUNT         2516 a         6299 c         5986 g         2566 t
ORIGIN      
        1 caccggccgg cgccccggat ccctacgcgt cgatcctcgc cgacgcaccg ccgggcgcga
       61 cggtcgcggt gtgggtcggc cagcccgagc ggctcgacta cgcgcggcac cgcgtgatcg
      121 acctgcgcgc gccggcggcc gcgcggctgc gcgtgcaccg cttcgcgccc cacacctcgc
      181 agctcgccgc cctggtgtcc ggcgtcgccg cgtcgttcct gctgatcgag gacgacgacg
      241 tccggatcga gcgcatccac gacgacgtgg tgtaccgcct ggcgtgccgg cagccgcggc
      301 cgatgtgcga cgacgatctc gaggcgctcg ccgcgcagca tccgctcgtc gcccggcgcg
      361 gcaacctccg gctcatcgac ctcgggcgct gacccagctt gaccaccgca gccggcggtg
      421 gcacacactg ccaatgtccg cgtccgcttg tgaggtttgc tcgtcgtccg agtcccggcc
      481 gatgttcgac aagctgaacc atcacttcgt gcgctgccgc ggctgcgggc tcgagcggat
      541 ctcgccgcag ccgaccgacg agaccctggc ccggatctac ggcgcccact actacgacgc
      601 ctggggcctg cacgacaacg aggacaccgt cgcccggctc aagaagcgca cgttccagta
      661 cgtgctcggc aagctgccgc ggccggcgcc gcccgcgcgg ctgctcgact gcggcgccgc
      721 caccgggttc ctgctcgagg tcgcgcgcga ggtcggccac gacccctacg gcctcgagct
      781 gtccgagttc ggcgcgagcg cgatcgccgg caagttcggc gccgaccacg tgttctgcgg
      841 cgagctgccg cacgcgaagt tcgccggcgt cgagcccggc ggattccagg tcgtcacgat
      901 gtgcgactac atcgagcacg tccgcgatcc gcggcagacg ctggcgctcg cgcggcgctg
      961 gctcgccccc ggtggcgcgc tcgcgatcac cacgccggac gccggatcgc cgtcgcgcag
     1021 gctgctcggc accggctgga cgcattacaa gatcgagcac ctctactact tcaacaagcg
     1081 caacctggag cgcctgctcc aggaggtcgg cttcacccgc gtgcggttct atccgctgtg
     1141 gaagtcgctg acgctcgact acatcggcca tcagttcgag gtctatcccc accgcgcgct
     1201 gactccgttc gcccgcgcgc tgcgccgcat gccgcgcgcg ctccgcgccc tgccgctgcc
     1261 gttcaccacc ggcgagctgc tggcgaccgc acatgcttga gcgctggtgg tggcgctggg
     1321 gctgggtcgt gcgatggagc ggcaccgcgc tcggcgtgct ctacgtcgcg cggctgatcc
     1381 accccggcaa gctcgcggcc gcgttctcgg tggtctcgct cggcgccgtg ctcgggtcga
     1441 tcgcgatcgt cgcggccggc atcgagctcg gcgcggtgcg ctggcggatc gcgctgcgcg
     1501 cctacggtgc gcggtcccag cccgggctgc tcaccgcctc gcggctctac ttcatcgcgg
     1561 tgttctacaa caccttcctg cccggcgcgg tcgccggcga cgtcgtgcgg ggggtggcca
     1621 cccgggacag cttcggcgag cacggcgcga ccggcgcgct cgccgtcgtg ttcgtcgagc
     1681 ggctgctcgg actgttcggt gtattcgccc tgatcctcgc ggggatctgg ctcgtcgggg
     1741 atgcgctggg cgaccgctcg atgtggtggg gaagcctggc gggcggcgcc gcctcgctcg
     1801 ccgcgctgtt cctgctgccg ctcggacgcc ggctcggccg gttcctgccc gcgccgctcg
     1861 cccggatcgc ggagcgcctc cccgtcgtcg tgcggccgcg cgacttcgcg gcggcggcgc
     1921 tgctgtcgct cgcgacccag ctcgccgcgg tgatcgccgg ctggctggtg ctgcgcgacc
     1981 tgcatccgcg gatcacgttc ggcgatgcgc tgctgatcct cccggtcgcc gcggccaccg
     2041 cgttcctgcc gatcaccgtg ggcgggaccg gcgcgcgcga ggcggcgttc gtgttcctgt
     2101 gcggcagggt gctgggcatg gcgagcgacg acgcggtggc cgcctcgctg ctgatctggc
     2161 tcgcgaccct ggtgatcgga gcgttcggcg ggctcttggc gctgaccggc cgcggcgcga
     2221 gtgacgatca gtcgaagctg cgcacggcgt cgatcacccg gccctgatcg gcgtcgtcga
     2281 ggtcgtagta gaacggcagc cgcacgagct cgtcggcgat cacctcggtg accgggcaca
     2341 tcccgggccg gccgccgtgg cgcagcccca tcggcgacag gtggagcggc acgtagtgaa
     2401 acaccgcctg gacgccgcgc gcccgcaggt ggtcgaggag cgccggccgc cgcgcgccgg
     2461 ggacccggag gtagaacatg tggaacgcct gctcgcagtg cgccgggacc accggcagcc
     2521 ggacgtcgtg cgcgccggcc cagccggcga gctcgctggc gtagcgctgc cagatctgcg
     2581 cgcgccggcg ctggatctgg tcgcgggcct cgagctgggc gaccagcagc gcgccgagca
     2641 gctcggacag cacgtagctc gagccgacgt cgacccaggt gtacttgtcg acctggccgc
     2701 ggaagaactt gctgcgatcg gtgcccttct cgcgcaggat ctcggcgcgc tcggcgaacc
     2761 gctcgtcgtt gatcagcagc gcgccgccct cgccgcacga gaagttcttg gtctcgtgga
     2821 agctctgggt cgcgagcgcg ccgagcgccc cgagcggccg cccgcgatac gcgccgaaca
     2881 ggccgtgggc gttgtcctcg atcaccaccg cgcccgaggc gcgcgcgatc tcgccgagcg
     2941 cgtccatctc gcagccgacc ccggcgtagt gcaccggcac gatcgcccgg gtccgcggcg
     3001 tgaccagctc ggcgacgcgg gcctcgtcca ggttcagcgt gtcgggccgg acgtcggcga
     3061 acaccggccg cgccccgcgc agcacgaacg cgttggcggt cgacacgaac gtgaacgacg
     3121 gcaggatgac ctcgtcgccc ggaccgaggt cgagcagcag cgccgcgatc tccagcgcgt
     3181 gcgtgcacga cgtcgtgacc agcgcgcgcc ggaccccgac cgccgcctgc agcagctgct
     3241 cggcgcggcg gccgaacgca ccgtcgccgg cgagccggcc ggtcgcccac gcctcctgga
     3301 tccgctcgag gccgatcgcg gccgggcggt tgaacgggat catggcgcgc ggccccagat
     3361 gtggtcgggc gcgcgcggac cggtcatcac catcaggtcg atcaccgaca cctgcggatc
     3421 gtacggcgga tacagctgct cgtagtctgg atagtcgtac accatgtact cgaggccgat
     3481 gccggcctcg gcgaacagcg cctcgtcgag gtaggcccgg gccgacggtc cggagatgta
     3541 gtggttcgcc gagaccttgt cgaggatccg gatcagccgc tcggtccgtg ttccggacac
     3601 gccgagctcc gacgaccgca cgaaccgcgt ggtcgtgatc ccgagcgcgc gggccagcgc
     3661 gagcgtggtc tcgatcgtga agtcgaccag ccgctcgggc cggccggcgt agaacccctc
     3721 gatcatcgcg gcgtggtcgg cgaagtacgg cgccttgccg tagcacccgc gcagggtcag
     3781 ggcgtgcttc ttcgcccagt cgcgggtcca cacgatccgc gcgtcggcga gcgccagccc
     3841 ggtggtgacg ttgcccttgg acgccaccgg gatggtcagc cactgggtcc ccgacggcgt
     3901 cttgatccgg ttgcggttgc gccagccgtg cttgtcgtac tggacgtcgt cgtagaacac
     3961 gaacacgtcg gcgcggtgga tcaggtcgaa gtagccgcgc cacgggatgt acgatggctg
     4021 gaggatgacc ccgacggtgc gctcggtcat cggccggtca cctcggctcg gcggccagcg
     4081 cgagcaggta ctgcccgtac tcggtcttgt ccagcgcccg ccccaccgca tgcagcgtgt
     4141 cgcgatcgat gaagccgcgg cgatacgcga tctcctcgag acagccgatc ttgaggccct
     4201 ggcgggtctc gacggtctgg atgaagttgc tggcctggag caacgcatcc ggggacccgg
     4261 tgtcgagcca cgccgtgccg cggcccagca cgatcagccg cagcgtgccg gcctcgaggt
     4321 agacccggtt gacgtcggtg atctcgagct cgccgcgcgc cgacggccgc agccgccgcg
     4381 cgatctcgac gacctcgttg tcgtagaagt acagcccggg gaccgcgtag tgcgaccggg
     4441 gctggcgcgg cttctcctcg agcgagatca cctcgccggt cggcgtgaac tcgaccacgc
     4501 cgtactgcga cggatccttg acgtagtagc cgaagatcag cgcgccgctg gtcagccgcg
     4561 ccgcggtctc gaaccgctcg gtcaggccca tgccgtagaa caggttgtcg cccagcacga
     4621 gcgccaccgg atcgctgccg atgaagtcgg cgccgatcag gaacgcctcg gcgatcccgc
     4681 gcggcgcggg ctgcacggcg tagtcgatcc ggatcccgaa gttgcggcca tcgcccagcg
     4741 tggcctcgaa cgcctcgcgg tcgcgcggcg cgcagatcag caggatctcg cggatcccgc
     4801 cgagcatcag cgtgctcagc gggtagtaga tcatcggctt gtcgtagacc gcgagcagct
     4861 gcttgctgac cgcgcgggtc atcggataca gccttgtccc ggcgccgcct gcgaggacga
     4921 tccccttcat ggggccacct gaacgcgaac catgcggacg gagcctaaca gaagacgcgg
     4981 agcgccgcgc gttcagcgaa tgcgatacac ccggtaccac gggcggatca cgagcaggtc
     5041 ggagcgctcc gcggccatcg tctcgagcgt gacccagggc ggcgtgtcga ggatcacgag
     5101 ctcgccgggc ttcgcccact tcttgaacag ccgcacgtcc tggtcgtggc gggtgccgat
     5161 cgcgaacgtc cacaccgacg acaccagcgg tcgccgcatc gcgatcaggt agccggccgg
     5221 cagatcgtac aggaacagcg cgcgcggacc ctggacatag ctctcgatgt ccgaggtcag
     5281 gtcgcggagg atctcggcct tggcgggcgt cgtgtacagc ccgcggtacg gtccggaggt
     5341 gacgcgctcg gtcagctcgg accccgggcc gtcgcggtag tagccgccgt ccgagaccag
     5401 gcacgcgagc agcgcccaca tcgcggcggc cggtgccagc gcgaggtacg ggtgaagcgc
     5461 acgccacccg ctgcgcgccg cggtctggac gatgaacatc accaggagga tcgccgtcgt
     5521 gatcgcggcc ggatacagcc cgatcccggc ggcgtacgcc cggttgctgc tcgacaggca
     5581 gatcaccgcc ccgctgacca tcgccggcac gatcacggcg aactccagca cccgggcgac
     5641 gcggcgctcg cggatcaccg cagcgagcac cggaccgaac agcgcgaagc acgtcaggta
     5701 cgccatgctc gcgaggtgac cgccctggtc cgagccgcgg gcgagcagcg ggatcgccac
     5761 ggccccgcac gcggcgagca gcggcaacca gcgcgcgatc acgatcaccg cgacgatcgc
     5821 gatcagcgcg cgcggcagct gcgggtgcag ctcgaggagc tcctggaacc gcgcccacgc
     5881 gacggggagc gagaacgcat ccccggcccc cgcctggtag gccagctcga tccggaggtt
     5941 gcggaggccg gcgcgcacga acagcggcga gatcagagcg acgaacgcca gcccgccggc
     6001 ggcgtacatc gcgactgccc ggacgcgctg accccgcgcc agggcgcaca cggcgatcgc
     6061 ggtcgccgcc gtcgtgacca gctgcgacgg gtaggcgaac gttgcggcgc catgggcgaa
     6121 cccggcccac agcagcgggt ggcgccacca cctgcgcggc ggctcgccga acagcagcgc
     6181 gcgggcgatc acgaacagcc cgctcgtcat cagggccatc gacagcgtgt tgtagctgag
     6241 cccggggatc ccgtgaggca gcaccagcat gcacatcgcg ccgcacagca gcgccgccgg
     6301 ccgcgggatg cagcgccgtg ccagcgcata cgccgaccac ccgaccagcc cgaacaccac
     6361 cacgtacagc gcgcggatga acagcacgat gccggtcgtg ccgcccgcga tccacaggta
     6421 cgccttgatg aacggataga gcagcagccc ggcggtctgc gcgatgttga gctcgtcgac
     6481 gaacgggcgg tcgccgagcg cgaaccgcag cggcagcgcg atgtagaacg cctcgtcggt
     6541 gaagtcgatc ccgtgcagca tccgccagca cagcacggcg atcgcagcga cgcagatcac
     6601 gattgcgatc ggagttgcat acgagcgtct cacggctacc gcccggacct atatcacacg
     6661 ccccggagac acggcacgcg ccggcggcgc gggcaccggc acgccgatcg cgacccggca
     6721 aattccggcg cgcgcgagcg ccgagcgccg aggggcgagc gccgagcgcc gatgacgctg
     6781 tccccctacc gcgatccgcg caacgaggag ccgcttcgcg ccatgaggaa ggttgccccg
     6841 caccgcaaga gccgatagcg tccggagcca ggtaacgaga tgagcgacag gtaccgtgga
     6901 tgcgacgacc gagcgtcacc cctgcccctg ggcctgcccc tgggcctgcc cctgggcccg
     6961 cccctgcccc cccccggggt accgtgaagt attcgttcgt cgttccgatc tacaacgatg
     7021 gtgcgctcgc tcgcgacttc tgtcgcgaag tcgagatcgc gtttcgcgag tacctcggcc
     7081 acggcgatct cgcgcgcgat ctcgaggtca tcttcgtgga cgatggcagc accaacgact
     7141 cgccgcagct gctcaagcag gtctgtgacg agttccggtt tgccaggctg atcctgctgg
     7201 cccggaactt cggccagcac atcgcggtca cggcggggta ccaggcagcg cgcggcgact
     7261 acgtcgggat gctcaacgtc gaccaggagg atccgcccag cggtctcccg ctgctgctcg
     7321 acacgctccg ggacagcgag tacgacatcg tcgggggccg ctaccagcgc cggtgggtgc
     7381 ggctgacgac gcgcatcacg tcgtatctgt ttcacaccct gatgaaccgg ctcactggct
     7441 atcgcacgcc ggtcaacgcc tcgacgatgc gggtcatgac gcgccgggcg gtcgacctgt
     7501 acaacacgct caccgagaag agccgatacc tcccggggct cgagcgctgg ctcggcctgc
     7561 gctatggctg ggtgcaggtc gtgcaccagc cgcgccgcgt cggcaagtcg agctacaact
     7621 ttcgccggcg gctgcggatg gccatcgcca cgatcatctc gttctcggac tttccgctcc
     7681 ggctggcggt caagttcggc ctgctggtcg cggcgagcgg cgtcttgctg gggaccgcga
     7741 tcatcgtcga caagctgttc ttccgggcgc tgatgcccgg ctatacctcg acgatcgcga
     7801 ccatcgtgtt cctgggcggc gtgcagatcg ccgtcaccgg ggtcgccagc ctgtacatcg
     7861 ggcgcatcct gtccgaggtc cagggccgcc cgctgttcat cgtccgcgag acctacggcg
     7921 gagcggtccg ggagctcgag atgccggcgc cgagcccgcc cggtgccgtc ctgcgcacga
     7981 ccagcgtcga cgccggctcg cgcgccacgg gggatagctc gtgacgccgc gatcgtcgtg
     8041 agtggaaccg gcaggggctg gatgggcacg ctgcggccgt ggcacgtgag cgtgggcgtc
     8101 ttcgcgctgt gtctcgctct gctcctggtc cggtcctacc acccgctgta cgacgctcct
     8161 gcgaccaacg gggacgagac ctgctcgctg gcgcaggcca tgaacagcct ccgtctgggc
     8221 agcccgggct atggctggac cgcgcagctg aagccgtgga cggccgggag gttcaaccag
     8281 ccggtgtacc tcgtggtgta cgggacgctg atgctctacg tccaccgctg cggcggttga
     8341 cggccgcccg ggtctgctcg ggcgtgctgg tggccgcggc gctgttcctg tgcctggtcg
     8401 tgctgtgacg cgcgctgcgt tcgcgcgagc agcgatcgtg gctcgaccgc gcccgtgtcg
     8461 ccgccgcggc aggaggcctg gtctggctgt tcgccggggg cgagaacttc ctccacgtcg
     8521 cgtcgttcgc gcgcgtcgac gccctggccg tgctcgcggt gtgcgccttc caggtcgtcg
     8581 cggcgggatt cctcgcccgg ccgaccgagc tcgggctgtg gtacctgctc gccgccccgg
     8641 gccggcaaca gctcgtgcaa ggtggcccgc gcgctcggca tcgtgatcgc cacctcgctg
     8701 gcggtgttcg tgctcgtcaa cttcgtgctg cagcacacgc cgcacagcat tcccaacgtg
     8761 gcctttgccg ccgagctgac gaacgtcgtc tcccgcgcca cccacccgtg gatggtgaag
     8821 gacctgatct ccctgctgcg caattttccc ggcgtcctcc cgaccgcggg cttcccgccg
     8881 ccggctcggt cctgctcctg cgtggccatg tcaggccatt cgcggtccag ctggtgcgcc
     8941 tggcgctcct gctgttcgtc acctgggtga tgttcgcggt gcggccggtc tacgcgccgt
     9001 tcctgctgct ggcgatcctg ctgccgtttc tcgaccagcg agcacctggc cgactccgcc
     9061 ccgttcgtcg tccgctacgc tgacgacacc ggatttcgct tgcagcagcg gttcgtctcg
     9121 gcggtgaggg gcttcggtcc caccggggag taccagtacg ccgatcgcgc ttcgacaccg
     9181 atcacggtct acgcgatcga gccagttcgc tgacgtggta ttccgcgacc agcacggttg
     9241 ctccacgccg cgagagccga tagggtcgac cggtcatgag cgaccggtac caggacgtca
     9301 tccaggcaaa catcgccgtg cactcgaagg tcgcggcggt gtattccacg accgagccgc
     9361 atttccggcc cgagaatctc gcggtcgtcg agagcgtgct gaagcgcctc gtcgcggaga
     9421 cccgcgcgac gtccctgctc gacctcgggt gcggcaccgg cttcatgatc aacctcgcga
     9481 agcgccatgt cgcgaggatc gtcggcatcg acgtcacgcg cgcgatgctc gatcaggtcg
     9541 acacctcggg tcccgcccgg atcgagctcc acgagcacga caccggaagc ttccacgtcg
     9601 agccaggtag cttcgacctg gtgaccgcct actcgttcct ccatcacctg tacgacatcc
     9661 ggccgaccct cgcgaccgcg gcccgggcgt tgcgcaaggg cggccggttc tacgtcgatc
     9721 tcgagccgaa cttccacttc tgggaagcga tctcgcggct tccgaccggc gcgggcgcgc
     9781 cgagctatga cccgctggtc acccgcgaga tcgacgcggt cgtgcacaag gacgaccaga
     9841 tgaacgagac cttcggcatc cccaacacgg tgttcaacga cgccgagtac ggcaagaaca
     9901 tcgccggtgg cttccaggaa gatgccctgc gcgacgcgct gcgcgaggtc gggttctcgg
     9961 acgtgacctt cacctactac tggtacctcg gccaggcgct gctggtgaac gacaagtcca
    10021 agtcgcgcga ccagtgcctc gccgacgccg cgctggtcga cgcgctgctc cagaaggtca
    10081 tgccgctgtc gcgccacctg tacaagtaca tcggcttcgt cgcgatccgg tgacccgacg
    10141 gtcgccatga gggtcttgat catcggcaac cgctcgttca tcgcgctgcg actgcgcgag
    10201 cgtctgggga aggagcacga ggtgctgctc gccggacgca ccggcgacgc cgacgtgcag
    10261 ttcgatctga ccgccaccgg cgcgccgcct cgcgaggttc cccgctgcga cgccatcatc
    10321 cactgcgcgg cgagcttcga accgaacact cccgatggca tcctccgcaa cgagctggtc
    10381 aacggcgtgg gtgcgctgcg ggtcgccgag ctggccaccc ggatcgcatg cggtcggatc
    10441 gtccatctat ccaccgtctc ggtgttcaac gacgaccacg gccggctgcg cgactcctac
    10501 gccctgtcca agcgccacgg ccaggacaac ctgcagctgg tttgcgacgc ggctggcatc
    10561 gagctgtgca cgctgtgccc gaccgcgatc tacgacgagc ggggcgcggg gcgccatcac
    10621 cagccgctgc tgtaccggat cgtcgactgt gcgcgccggg gcgaggactt cccgctgtac
    10681 gggagcgcgg atccggcgcg caactacctc catgtccgcg atctcgccga gctggtcgcc
    10741 ggcgtgatgc gcgccggcgt gtccggcacc ttcccggtgg tatttccacg cgactaccgc
    10801 atcaccgaga tcgccgagct ggcgttcgcg gtcttcggca ccaccggccg catcgtccgg
    10861 cgtccggaca tgcccgacat tccggtcgta cgctatcccc ggtctcgcga gctgttcgac
    10921 cgcctcggct gggtcccggc gctcgatctc gaggccggct tccgcctcct gcgggatcac
    10981 catggctgaa ccggcgcccg cacggcggcg tcatgtcctc gtcaccggcg tgggcgcgat
    11041 catcggctac ggcgtcgtcc acgcgctgcg gcgcgcgagc taccccgtcc agatcatcgg
    11101 gatggacatc tacgggcacg cggtcggaca gacctggtgc gatgcgttcg agcgcgccat
    11161 cccggtcgca gatcccgggt acatcgcctt cctccgggac gcgatcgccc gccacgcgat
    11221 cgatctcgtc atcccggcga tcgagcagga cgtgaccttc ctgtcgcgcc atcgcgcgga
    11281 attcgcagcg accggagccc gcctggcgct caatgcggcg tcgctgatcg agcagagcga
    11341 cgacaaatgg cggatgcatc aggcgctcca ggcgctcggc tgcccgacga tcgacacgat
    11401 gctcgcgggc gagttcgacg cgctcgcagc acacctgggc ctgccgttcc tggtcaagcc
    11461 acgccggtcc tacgcgtcca aggggctggc ccggatccac gacgccgagg acctcgcgta
    11521 ccaccggcgc aagctcggcg ataacttcat ggcgcagcgc atcgtcggag acgccgactc
    11581 cgagtacacc tcgagctcgt tcggctacgg cgatggcacc gggtcgcgca agatcgtgtt
    11641 ccagcgctgg ctcgcccagg atggctcgac cgcgcgcgcg caggtggtcg acgagccgtc
    11701 gctcgagcac gagatggacc gcctgacccg ggcgtttcgt ccgctcgggc ccaccaactt
    11761 ccagttccgc cgccacgagg gtcgattcta cctgctggag atcaacgccc ggatctcgtc
    11821 ctcgacctcg ctcaagcgcg cgttcgggat gaacgaggcg gagatgtgca tcgcccacta
    11881 cctggacggc gagcgacccg atttcgggtc cgtgcgcaag gggaccgcgg ttcgctacat
    11941 cgaggacatg atctcgtatg acaaacctac cttctgacgc cggcgactag cgtatgtcgc
    12001 tgcgcatcgt cggcgtggcc gatacccacc tgttccacga ccgcctggcc gtcccggacg
    12061 gcgacgtgtt cgttcacgcc ggcgatctgt gccggggtgg ctcgctgcca gagctcgcgc
    12121 aggccgcggc ctggatccgc gccctgccgc atgccacgaa gatcgtcgtc gcaggcaacc
    12181 atgactggcc gttcgtgcga gaccggggcc gcgccatcga gctgctgggc cccgacgtca
    12241 tctacctgga agactccgga ctcacgatcg gagggctgag cttctggggc agtccctggc
    12301 agccgatcta cggcgcgtgg gcattcaacc tcgaacgcgg cgccgcgctc gcggccaggt
    12361 gggcgctgat cccggaccgc gtcgacgtcc tcatcacgca cggaccgccg cagggaatcg
    12421 gcgaccaggg cccgatctcc agccgcgccg gctgcgagga gctgagggcg cgggtcgagg
    12481 tcgtgcggcc cttgctccat ctgttcgggc acattcacca ggacggcggg ctgtggcagc
    12541 acgacggcgt cgtgttcgcc aacgtgacga ccgcggactg ccggcgcgag cccacggtga
    12601 tcgacgtcgc cgccgaggat cgccgggtgc acccggtcga ggtccctccc ccggcggggt
    12661 gatcctattc ggtcttctgg cgaaacttcc gctgctcggg cggcaggtag tactcctcga
    12721 gcgactccgg cacctgggcg tagaacccca tcttctggag cgccctgtcg ctcacgccgg
    12781 tgaggtcgac gccgctcgcg gccatcatct tcggaatgtc gagccgctgc ttcatgtacg
    12841 ggcggcacat gttgagcgtg atcgaatggc gccacgcggc ggtgtgattc tggccgcccg
    12901 cgtgccagac ccgcggatcc aggtacacgg cgctgccggc gggtgcgatg acgcgcctgg
    12961 cgttcgcgaa gaagtactct gccgtgggcg gctcgcgcgt gtgctgggag ccgggcaaca
    13021 cccacgtcgc gccgttctcg gcggtgaact cgtcgagcag cagcatgacg ccggcgttgg
    13081 tcgcgtagcc cgggatcagg cgaggacagt cgacgtggat gcgcgcggag tagttgctcg
    13141 cccccggcgg catcgacgac gacgtatagg cgtagacgat gcagccctcg ccgaggatgt
    13201 gctcgatgat cgacatcacg ccgcggtccg ccagcacctc gacgaacgat cgcgcgtggg
    13261 tacagcacac gaggaccatg ccgtagtcga cgtagtcctt gccgcgatga taagccacct
    13321 cggcggcgat cgcggcctcg agatcgcggc gaagctcggc cacgcgatcc gcggtcagca
    13381 cgccgggaac gatggcgtac ccgtcgcgcg cgaggacctc caggatagct tcatcacggg
    13441 ggcgagcggt catggcgttc attccgtgcg gtgaggcgcg accggcgacc ggacgtatat
    13501 cacggccaga catcttgcgt tagggtgccg ccatggctgg gttcgagccg aaacgcttcc
    13561 tgatcaccgg cggcgcgggg ttcatcggct gcaacctcac ccgcgcgctc ctggcggacg
    13621 cggccgcccg cggcgtcgcc aggatcgtcg tgctcgacgc gctgacctac gcggggcacc
    13681 gggtgaacct cgacggtgtc ctgggcgatc cgcggctcga gttcgtcgag ggcgacatct
    13741 gcgaccgcgc gctggtcgag cggctgttcg ccgcgcacgc gatcgacgcg gtgctccacc
    13801 tcgccgcgga gtcccatgtc gatcgcagca tcgaggcgcc cgatgcgttc atccgcacca
    13861 acgtcacggg cacgttcacg ctgctcgacg tcgcgcgccg ggcctgggcc ggccggtgcg
    13921 atgcgacgcg gttcgtccac gtctcgaccg acgaggtcta cggcgcgctc ggcgagaccg
    13981 gcgcgttcac cgagaccaca ccgtacgccc cgaactcgcc gtacgcggcg tccaaggcgg
    14041 cgtccgatct gctggcgcgc tcgtacttca agacctatgg cctgccggtc gtgaccacga
    14101 actgctgcaa caactacggt ccgtaccagc tgcccgagaa gctgatcccg ctgatgatcg
    14161 ccaacgcgtg cgagggcaag ccgctgccgg tctacggcgc cggcaagcag atccgcgagt
    14221 ggctccacgt cgaggaccac tgcgccggcc tgtggctcgc gatgacccgc ggcacgccgg
    14281 gcgagagcta caacttcggc agcggcgacg agatgaccaa cctccagctg gtcgagctgc
    14341 tcgccgacct gatcgacggc cagctccagc gcgcgcgcgg caccacgcgt gcgctgatcc
    14401 ggttcgttcc cgatcgccag ggccatgact ttcgctacgc gatcgattgc tccaaggccc
    14461 ggcgcgcgct gggctggcag acgtcgcgga cgcttcaggt ccagctcgcc gagaccgtgc
    14521 gatggtacct ggccaatccg ggctggcgcg ccgcggtggc cagcgaggag caccaccgct
    14581 tcctgtcgcg gcagtaccgc gatcagcgcg gcggtccgga tccggcgggc acatcgaccg
    14641 gcacggactg agtggccgcg ccgcggcgcg ccggctcacc gccgcactgc cacgaccaga
    14701 cagctggtgc cgaacggcac gctccgaccg gcgcggatcc gccgcagctc gggggccagc
    14761 aggccgtcga tcgcgcgccg caccagcccg gacggcacgt gctgacgcgc cgccccccgc
    14821 tcgacggcgg cgtccgaccg gcggcccagc cacccccgca cgctgcgcag cacgaacacc
    14881 ggggcgacca gcggcgcgaa gaagtaggtc cggtacgcga cctcgaaccc gcagcgcgac
    14941 agcgtcgcga ccagctcgtc cagcgtgtag cggcggtagt gcccggcgag ttcgtcctcc
    15001 tccgaccaca gccagcggta ggcggggacc gtcacgccca gcgccccgtc ggggcggagc
    15061 acgcgatgga cctcgcgcac cagaccctcg tcgtcggcga catgctcgat cacgtcgaac
    15121 agcccggcgg cgccgaacga cccggcctcg aacccggcgt cctccagggt cgcgcacacc
    15181 acgctgcgga gcccgcgcgc ccgcgcgttg cgcgcccccg cgtcgcctgg ctccagcacg
    15241 acggtctcga tgtgcgcacg ctccagcgcc tggctgacgg cgccgttgcc accgccgacg
    15301 tcgagcagtg ggccggcgac gcccgcgcgc gcgagcgcgg ccacgatgca cgcgttgcgg
    15361 tgggcgaacc agaacgagtc cgactccacg ccgaggcaca cgtcgtggcc gtcggcggga
    15421 aagctcagcg ccggcgccgc cgtccgcgcg cgccagatcc cgtcgctgcc cagcgcgagc
    15481 ccgtggccga tccggccgag gtccattccg gcgccgtcca cgtgcgcgct caagcgggct
    15541 cgacccgctc cccggccgcg tcgcggatca ggcgcagcgc gaacccggtg tgatcgacga
    15601 accggccgcc ggcgtcgaac gtcgcgcgct caccggcggg cgtgacccaa ccgtgctgcc
    15661 gcgccggatt gccgtagacc agcgcgtagg gcggcaccga gcgggtcacc acgctgcccg
    15721 cgccgatcat cgcccacgcg ccgatcgtca cgccgcaccg gatggtggag ttcgcgccga
    15781 tcgcggcgcc ggtgcacacc cgggtcggcg tgatgtgcca gtcggccgcc gacttgagcg
    15841 agccgtccgg gttgatcgcg cgcggcacga ggtcgttggt gaagcacacg tgcgggccga
    15901 ggaacacgcc gtcctccacc gtcaccccgg cgaacaccga cacgttgttc tggatcttgc
    15961 agcggttgcc gatgtgcacg ccggtctcga tgtagatgtt cttgcccagg atacagtcct
    16021 cgccgatccg ggtgttctcg cggatctggc agtgcagcca gatctgggtg cggtcgccga
    16081 tcacggcgct cggcgcgacc accgacgttt cgtggatccg cgcggtcact gcggcctcgg
    16141 gacgaaggtc aacggcttca gcggcaacat cggcgctcct tggtgtggac ggcgatcatc
    16201 gcaggatctc ccgcgccgcg ccgacgacct cggcgacggc gggctcgggc agcccgaagt
    16261 acagcggcag gttgagcacg cggcggacga acgcccgcga ccgggcgagc tcgccgaccg
    16321 cgacgaaccg gccgcgggcc ggcgcctgct ggtcgatcgt ctcgggatac gtccgggcga
    16381 cgccgatcgc gcgctgctcg aacgcgccca cgcagtcgtc ggccgggcgc tcgcactcga
    16441 cgaccagcag gtaaccgttc tccgcgatcg cgctcgccgg cccgcgcagc cggcctgcga
    16501 tgccggtcca ctgcgcgcgg tacgccgcga ccagccggcg gcgggcggcg aggatctcgc
    16561 cggcgtgcgt catcatctcc agcaggtaat gggccgcaag cccgcccatc cgcgcgttcc
    16621 agccgacctg ctggtacgag tagtgcgccg accgcccgtg gttggccagg ctgcgcacgg
    16681 cggcggcgag cgcctcgtcg ccggtcacga ccgcgccgcc gtccatgcag ccgccgatca
    16741 ccttggccgg gtagaacgac agcgtcgcga gcgcggcgcc ggcgagcagc ggccggccgt
    16801 cgagctcgac cccgatcgcc tgcgccgcgt cctcgatcag gcagatgtcg tggccggcgc
    16861 agaacgtccg gatctcggac agcgcggccg acgcccagcc gtagaggtgg acgatgatcg
    16921 cgtggcggaa gccgtaccgg gcgtgggcgc gctggagctc gccgagatcg agctgcagat
    16981 cgtcggccga gatgtcgatc agcaccggga tggccccgag ctggacgacc gcctcgaacg
    17041 tcgcccagaa cgtcaggttg ggcagcgcga cgtgcatccc cgggcggacg ccggcggcct
    17101 gcagcgcgag cagcagcgca tcgctgccgt tgctgcacgc gaccgcggcc cgggtggcgc
    17161 agaaccgcgc gatctcggcc tcgagctgct gcacgccggc gcccccgacg aactcgcagc
    17221 gctcgaccac gccggcccag cgcggcgcga cggcgtcggc gaccagccgg gacacggggt
    17281 gcaggtcgat gaacgggatc atgcgggcag cgtccggttc tacgccagga tccgccggcg
    17341 atggtagagg tggcgtcgac cgaatcc
//
LOCUS       DASVOR010001399         2904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_422808,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001399 DASVOR010000000
VERSION     DASVOR010001399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422808"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..764)
                     /locus_tag="VF516_26630"
     CDS             complement(150..764)
                     /locus_tag="VF516_26630"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8111345.1"
                     /translation="MVAASSPSSFEEDQHVVLRAMAWQDFEALLAIRGERSGVRMYYL
                     DGEIEIVSPTKIHEGRKTTLARLLEIWAMESDIALNGFGSWTLKNEIREAGAEPDECY
                     IVGDSTEKDVPDLAIEVEGSRATGLSKPEIYRRLGVRELWTLKSDGRLLIRILEKGEW
                     SEHTRSKLLPKLDIAWLLSFLEVTPQSKAVRALRDALRTKKRRS"
     gene            complement(816..1622)
                     /locus_tag="VF516_26635"
     CDS             complement(816..1622)
                     /locus_tag="VF516_26635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8111346.1"
                     /translation="MESPSAESQRIFAPGILHDQVAIVTGGGSGIGLATAREMVRLGA
                     RVAICGRTQDKLDAAAEELRAAGGTAFARTCDIREPAQVEAFVTAVLGELGRIDILVN
                     NAGGQFPSPAQHISPNGFLAVIKNNLVGTFHVTREVANQAMIPARRGRVINVIANIYR
                     GFPGMAHTGAARAGVENLTMSLAVEWAQFHILVNAVAPGVIRSSGTARYPPALFERGT
                     RETPLKRAGTCEEVAAAIVFLASPAAQFITGATLRLDGGQSLWGHSWEIP"
     gene            1892..>2904
                     /locus_tag="VF516_26640"
     CDS             1892..>2904
                     /locus_tag="VF516_26640"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111347.1"
                     /translation="MRPSSEGGNKRSGSRYVLGDLLGRGGMAEVFAGHAVGDHGFQKP
                     VAIKRLLPELANDEVFVERLIEEAKLLVGMQHGHIVSVIDLAREGDDVFLVMEFVDGP
                     SLRQLIKARGARGLSLGVATYIVQSAAAGLEFAHARPGGAIIHADISPSNLLLTTSGE
                     VRVADFGIARREGLGQGVVEGKWAYMAPEQARGEPLTPRSDVFALGVVFYELLTGQHP
                     FGRAVTPNERDGQIQVIPPRVVKPSIPPGLDAICMRALAHSPRDRYGRMQQLIDAVVE
                     ERFASGLREGAADLAQAIREVAPKSDAAPPRTMHTERPVTLMTRSLLRDVTPPRRPSR
                     PSRP"
BASE COUNT          417 a          980 c         1079 g          428 t
ORIGIN      
        1 gccagggcga tcgcgcggcc gatgccgcgg ctcgcgccgc tgaccagaca cagcttgccg
       61 gtgagctcga ggtccacgcg cccagtatcc ccccacggcg ccgcgggagg tattctggga
      121 gggccagttt tggtgcggtc ctcctgacgt cagctacggc gcttttttgt acgaagcgcg
      181 tcgcgcagcg cgcgcacggc cttgctctgc ggcgtcacct cgaggaacga cagcagccac
      241 gcgatgtcga gcttcggcag cagcttgctc cgggtgtgct cggaccactc ccccttctcc
      301 aggatgcgga tgaggaggcg gccatcgctc ttgagcgtcc acagctcgcg aaccccgagg
      361 cggcgataga tctccggctt ggacaggccg gtcgcgcgcg acccttcgac ctcgatcgcg
      421 aggtcgggga cgtccttctc ggtggaatcg ccgacgatgt agcactcgtc gggctcggcc
      481 ccggcctccc ggatctcgtt cttgagcgtc cacgagccga agccgttcag cgcgatgtca
      541 gactccatcg cccagatctc gagcagccgg gcgagcgtcg tcttgcgtcc ctcgtggatc
      601 ttcgtcgggc tcacgatctc gatctctccg tcgaggtaat acatccggac gccggaccgt
      661 tcgccgcgga tggcgaggag cgcctcgaag tcctgccacg ccatggcgcg cagcacgacg
      721 tgctggtctt cctcgaagct cgatggagaa gacgccgcga ccatcacggg caaccatagc
      781 acgcagccga cggagccatc ccgggcaccg aggatctacg ggatctccca gctgtggccc
      841 cacagggact ggccgccgtc gaggcggagc gtggcgccgg tgatgaactg ggcggcaggg
      901 gaggcgagga agacgatcgc ggcggcgacc tcctcgcagg tgccggcgcg cttgagcggg
      961 gtctcgcggg tgccgcgctc gaacagggcc ggcgggtagc gcgcggtgcc cgacgaccgg
     1021 atgacgcccg gcgcgacggc gttgaccagg atgtggaact gcgcccactc gacggcgagc
     1081 gacatggtca ggttctcgac gccggcgcgc gcggcgccgg tgtgcgccat gccggggaag
     1141 ccgcggtaga tgttggcgat gacgttgatg acccggccgc gccgggccgg gatcatggcc
     1201 tggttggcga cctcgcgggt gacgtggaag gtgccgacca ggttgttctt gatgacggcg
     1261 aggaagccgt tgggcgagat gtgctgggcc ggcgacggga actggccgcc ggcgttgttg
     1321 accaggatgt cgatccggcc gagctcgccg agcacggcgg tgacgaacgc ctcgacctgc
     1381 gccggctcgc ggatgtcgca ggtccgggca aaggccgtgc cgccggcggc gcgcagctcc
     1441 tcggcggcgg cgtcgagctt gtcctgggtc cggccgcaga tcgcgacgcg cgcgccgagc
     1501 cgcaccatct cgcgcgcggt ggccaggccg atgccgctgc cgccgccggt gacgatggcg
     1561 acctgatcgt gaaggatgcc gggggcgaag atgcgctggg actcggcgct gggggactcc
     1621 acgcgccgac gtaaccacgg tccgcggacg gtgtgcagcg ccgaacgcgc gagggaccct
     1681 caaaaattca cgttccctca cggtggctga ccgccgctgt cagccctggg ggtggagccc
     1741 caggggggga cgtctctccc ccgatcgcct accccggtag tcagcggttt ccgtcgcaaa
     1801 ttgcgcaaag ccaagcggat gcgatcggtt agcccgggta tgggcgttgc agcattggca
     1861 tctcgtggcc gggatcgcta caactgggaa ggtgcgcccc tcgtctgaag gcggcaacaa
     1921 gcgctccggg agccggtacg tgctcgggga cttgctgggc cgcggcggca tggccgaggt
     1981 gttcgccggc catgcggtgg gcgatcacgg gttccagaag ccggtcgcga tcaagcgcct
     2041 gctgcccgag ctggccaacg acgaggtgtt cgtcgagcgc ctgatcgagg aagccaagct
     2101 gctggtcggc atgcagcacg gccacatcgt gagcgtgatc gatctcgccc gcgagggcga
     2161 cgacgtgttc ctggtgatgg agttcgtcga cggcccgagc ctgcgccagc tgatcaaggc
     2221 gcgcggggcg cgcgggctgt cgctgggggt ggcgacctac atcgtgcagt ccgcggcggc
     2281 ggggctggag ttcgcccacg cgcggccggg cggcgcgatc atccacgccg acatcagccc
     2341 gagcaacctc ttgctgacga cctcgggcga ggtccgggtc gccgacttcg gcatcgcgcg
     2401 ccgcgagggc ctcggccagg gcgtggtcga gggcaagtgg gcgtacatgg cgcccgagca
     2461 ggcgcgcggc gagccgctga ccccgcgctc ggacgtgttc gcgctcggcg tggtgttcta
     2521 cgagctgctg accggccagc acccgttcgg ccgggcggtg acccccaacg agcgcgacgg
     2581 gcagatccag gtgatcccgc cgcgcgtggt caagccgagc atcccgccgg ggctcgacgc
     2641 gatctgcatg cgcgcgctgg cgcactcgcc gcgcgaccgc tacggccgga tgcagcagct
     2701 gatcgacgcg gtggtcgagg agcggttcgc gagcggcctg cgcgagggcg ccgcggatct
     2761 cgcccaggcg atccgcgagg tcgcgccgaa gtccgacgcc gccccgccgc gcacgatgca
     2821 caccgaacgg ccggtgacgc tgatgacgcg gtcgctgctg cgcgacgtga cgccgccgcg
     2881 ccggccgtca cggccctcgc ggcc
//
LOCUS       DASVOR010001400         2768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_160229,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001400 DASVOR010000000
VERSION     DASVOR010001400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2768
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160229"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..>2768
                     /locus_tag="VF516_26645"
     CDS             36..>2768
                     /locus_tag="VF516_26645"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HEX8111348.1"
                     /translation="MRHRIMDILLVASPYDSFILEEAGQIGERVMGEFRNLDLHYGPG
                     LTTVPTGGEALDLVREPSRFNLIISGLTLGDMNGAELARRVKALGLGIPVVLLAFDTR
                     EVQTFAEHHDLSAVEKVFLWQGDARILLAIVKYVEDRRNVVHDTQAMGVQVIIVVEDN
                     VRYYSSFLPVIYAELLHHSRRLVSEAVNVSDKIMRMRARPKILLCGSYEEAWAAFTQF
                     QDDVLGVISDIEFPANGEPSPTAGLDFARRVRARWPDVPVLLQSSNPAHTAAADAVGA
                     AFLLKGSPTLLADLRRFMIDYFGFGDFVFRRPSGQMVGMAHDLKSLEEWLRAAPVDSI
                     AYHAARNHFSSWLKARTEFALADELRPRKVSEFPDPEGVRSYLIETIARYRRARSLVA
                     VADFDRTAFDPRHDFHRIGGGSLGGKARGLAFMRMLLGQRPVTEGLDAKVTVPSAVVL
                     ATDVFDRFLDDNDLRDLALHADDDAELERRFLAARFPPDVRDDLAALLPRIDYPLAVR
                     SSSLLEDSQHQPFAGIYDTFMLANRGPLGARLDPLIRAIVRVYASMFSRRAKDYIAAT
                     PYRLEEEKMAVIVQRAAGARHGSRFYPDLSGVARSHNFYPAAPLTAADGIAAVALGLG
                     KTVVGGGNCLRFCPRYPRHLVQFSSVRDVLNNSQRTFWALDLAGGADMREVRYELDAA
                     EADGTLAAVASTYSPENDAIYDGVSRAGIRVVSFAEILKHDSFPLAAVLDRLLAIGHW
                     GMGGPVEIEFAADLAARELAFLQLRPLALSYEMLEVVIGEVEPDRLICRSSRVLGNGR
                     IDDLRDVIVVDRQRFARASSRQVAAELAQCNAELSGHPYLLIGVGRWGSADPWLGIPV
                     TWEQISGARAIVEAGFADLEVTPSQGTHFFQNLTSFNVGYFTVNPDAGDGFVDWD"
BASE COUNT          372 a         1046 c          928 g          422 t
ORIGIN      
        1 ccgtgcaccg cggcctgcag gatttccacc agctgatgcg gcatcgcatc atggacatcc
       61 tgctggtcgc cagcccgtac gactcgttca tcctcgagga ggccggccag atcggcgagc
      121 gggtgatggg cgagttccgc aacctcgatc tgcactacgg ccccgggctg accacggtgc
      181 cgaccggcgg cgaggcgctc gacctggtcc gcgagccgag ccggttcaac ctgatcatca
      241 gcgggctgac gctcggcgac atgaacggcg ccgagctcgc ccgccgggtc aaggcgctcg
      301 gcctcggcat cccggtcgtg ctgctcgcgt tcgacacccg cgaggtccag accttcgccg
      361 agcaccacga cctgtcggcg gtcgagaagg tgttcctgtg gcagggcgac gcgcggatcc
      421 tgctcgcgat cgtcaagtac gtcgaggacc gccgcaacgt cgtgcacgac acccaggcca
      481 tgggcgtgca ggtgatcatc gtcgtcgagg acaacgtccg ctactactcg tcgttcctgc
      541 cggtgatcta cgccgagctg ctccaccact cgcgccggct ggtgtccgag gcggtcaacg
      601 tgtccgacaa gatcatgcgg atgcgggcgc gcccgaagat cctgctgtgc gggagctacg
      661 aggaggcgtg ggccgcgttc acccagttcc aggacgacgt gctcggcgtg atctcggaca
      721 tcgagttccc cgccaacggc gagccgtcgc cgaccgcggg cctcgacttc gcgcgccggg
      781 tccgcgcgcg ctggcccgac gtgccggtgc tgctccagtc gtcgaacccg gcccacaccg
      841 ccgcggccga cgcggtcggc gccgcgttcc tgctcaaggg ctcgccgacg ctgctcgccg
      901 atctgcgccg gttcatgatc gactacttcg ggttcggcga cttcgtgttc cgccggccga
      961 gcggccagat ggtcggcatg gcgcacgacc tcaagtcgct cgaggagtgg ctccgggccg
     1021 cgccggtcga cagcatcgcg taccacgccg cgcgcaacca cttctcgagc tggctcaagg
     1081 cgcgcaccga gttcgcgctc gccgacgagc tgcgcccgcg caaggtgtcc gagttccccg
     1141 atcccgaggg cgtgcgcagc tacctgatcg agaccatcgc gcgctaccgc cgcgctcgca
     1201 gcctggtcgc ggtcgccgac ttcgaccgca ccgcgttcga cccgcgccac gacttccacc
     1261 ggatcggcgg cggctcgctc ggcggcaagg cgcgcggcct ggcgttcatg cgcatgctgc
     1321 tcggccagcg gccggtcacc gaggggctcg acgccaaggt caccgtgccg tcggcggtcg
     1381 tgctcgcgac cgacgtgttc gaccgcttcc tcgacgacaa cgacctgcgc gacctcgcgc
     1441 tgcacgccga cgacgacgcc gagctcgagc gccggttcct cgcggcgcgg ttcccccccg
     1501 acgtgcgcga cgacctcgcc gccctgctgc cgcggatcga ctacccgctc gcggtccggt
     1561 cgtcgagcct gctcgaggac tcgcagcacc agccattcgc cgggatctac gacacgttca
     1621 tgctcgccaa ccgcggcccg ctcggcgcgc ggctcgaccc gctgatccgc gcgatcgtcc
     1681 gggtctacgc gtcgatgttc tcgcgccgcg ccaaggacta catcgcggcg accccgtacc
     1741 gcctcgagga ggagaagatg gcggtgatcg tgcagcgcgc agccggcgca cggcatggca
     1801 gccggttcta ccccgacctg tcgggggtcg cgcggtcgca caacttctac ccggcggcgc
     1861 cgctgaccgc cgccgacggc atcgccgcgg tcgcgctcgg gctcggcaag accgtggtcg
     1921 gcggcggcaa ctgcctgcgg ttctgcccgc gctacccgcg ccacctcgtg cagttctcgt
     1981 cggtgcgcga cgtcctgaac aactcgcagc gcacgttctg ggcgctcgac ctcgccggcg
     2041 gcgccgacat gcgcgaggtg cgctacgagc tcgacgccgc cgaggccgac ggcacgctcg
     2101 ccgccgtcgc gtcgacctac tcgccggaga acgacgcgat ctacgacggc gtgtcacgcg
     2161 ccggcatccg cgtggtcagc ttcgccgaga tcctcaagca cgacagcttc ccgctcgcgg
     2221 ccgtgctcga tcgcctgctc gcgatcggcc actggggcat gggcgggccg gtcgagatcg
     2281 agttcgcggc cgacctcgcc gcgcgcgagc tcgcgttcct ccagctccgg ccgctcgcgc
     2341 tgtcgtacga gatgctcgag gtcgtgatcg gcgaggtcga gcccgaccgc ctgatctgcc
     2401 ggtcgagccg cgtgctcggc aacggccgga tcgacgacct gcgcgacgtc atcgtggtcg
     2461 accgccagcg cttcgcgcgc gcgtcgagcc gccaggtcgc cgccgagctc gcccagtgca
     2521 acgccgagct gtccggccac ccgtacctgc tgatcggcgt cggccgctgg ggctcggccg
     2581 atccgtggct cggcatcccg gtcacctggg agcagatctc gggcgcgcgc gcgatcgtcg
     2641 aggccgggtt cgccgacctc gaggtcacgc cgtcgcaggg cacccacttc ttccagaacc
     2701 tgacctcgtt caacgtcggc tacttcacgg tcaaccccga cgcgggcgac ggcttcgtcg
     2761 actgggac
//
LOCUS       DASVOR010001401         5142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_511038,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001401 DASVOR010000000
VERSION     DASVOR010001401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511038"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VF516_26650"
     CDS             <1..418
                     /locus_tag="VF516_26650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111349.1"
                     /translation="DHGRRRGIGVALGRVAGLGRRELAAGAGAVLLRGVRHLVREQVL
                     PARIVRPVLAAPEHDVQAGRERAGLDAICMIRRGVSGVNPHAGQIAAERLLELGARGV
                     GQRIPCTEILDQRVLSGARGRHGGLATRPRGSLRFT"
     gene            744..1088
                     /locus_tag="VF516_26655"
     CDS             744..1088
                     /locus_tag="VF516_26655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111350.1"
                     /translation="MKASFRALIVTQTTDTFAVGVREMRPEELPAGEVLIRVAYSAVN
                     YKDALVCTQNGRVAQTSPLVPGIELTGGSSSQATRVFEPAIGSLPMTSATESAFLSMV
                     DSVNMRVYPAIS"
     gene            1085..1756
                     /locus_tag="VF516_26660"
     CDS             1085..1756
                     /locus_tag="VF516_26660"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8111351.1"
                     /translation="MSALAPELGFPQALVLSGGLTAMMSLSELEKAGLTPQNGPVLVT
                     GATGGVGSLAIGLLSQRGYTVAASTGKPDAADYLRRLGASEILNRAETSAESSRPLEA
                     ERWAGSIDSVGGATLAYLMRTTKRGGAIAAVGVAGGAAFHGTVFPLILRGIKVLGIEM
                     PSTPLATRRQLWEQLQKEAGLLSLADSILREVALDDVPRVSQAMLRGQRQGRILVRLG
                     EPEST"
     gene            complement(1928..2518)
                     /locus_tag="VF516_26665"
     CDS             complement(1928..2518)
                     /locus_tag="VF516_26665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111352.1"
                     /translation="MKALLTALVGSALLGGCVVGSDSSQPSSDPSTSAVDPANTRSTT
                     TLPPIPEDEEYAFFRDRTFTFQIPGFFTAQAETFFIWNLGTAIQHNAPYPSSTHGKLF
                     AVFAQGPAGATHHVDGQPNFDHYHIITQEEGIRTFDGFLVFPGPNFNAATFVPPRSES
                     GLNAAIAAGVLNPPLTSTAAGLGPLVIKVPVSEFEH"
     gene            2924..4744
                     /locus_tag="VF516_26670"
     CDS             2924..4744
                     /locus_tag="VF516_26670"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8111353.1"
                     /translation="MDDNDELGLTAVTAVVPGDAVDESPPGHLQLFVISGGTFAAHPL
                     KEAGTATIGRGRQCDIEVDDRSISRRHAILSVGETVTIEDLGSANGTFVRGERIALGS
                     PAVISVGELVVLGSANIILQRRAQPVPPRKVWSRESFELLLEEECAREARYGAEFALL
                     CIHADRRAPPGFVEDTIAELLRESDIVARYGPHEYEVLLRDTPAANTDGAARRIDSKL
                     LERGLRCQISVACCPRDGRTASQLSAKAQGPVVKDRATGARADVVVSDPRMRGLHRLV
                     DQVAGSSISVLLLGETGVGKEVFARAVHRASPRAAGPFVEVNCAALTETLLESELFGH
                     ERGAFTSAVSAKPGLLEMADGGTLLLDEIGDMPLATQVKLLRVIEDSQLRRVGGLKPR
                     HIDVRFVAATNSDIEAQVEQGTFRRDLFFRLNGVTILIPPLRERLAEIEPLARAFIRG
                     CSSPGGGPRPELAPEALEWLRHHPWPGNVRELKNVIERAVLLCGSGPILPEHLSTRRP
                     TVQTTADEATTRPSPWPRDARPHAATDVDVRIPMFAARPRKGSQEEAQWIMQTLERAG
                     GNQTVAARLLGISRRTLVNRLNDYEHVHRPRKKPKAHPSE"
BASE COUNT          814 a         1707 c         1848 g          773 t
ORIGIN      
        1 cgatcatggc cgccgccgcg gcatcggcgt tgcgctcggc cgcgtcgccg ggctgggtcg
       61 tcgcgagctc gccgcggggg ccggcgccgt gctgctgcgc ggtgtgcgcc acctcgtgcg
      121 cgagcaggtg ctgcccgcgc ggatcgtccg gccggtactc gccgcgcccg aacacgacgt
      181 gcaggccgga cgcgaacgcg cgggcctgga cgcgatctgc atgatccgcc gcggcgtctc
      241 cggtgtgaat ccgcacgccg gacagatcgc ggccgagcga ttgctcgaac tgggcgcgcg
      301 gggcgtcggg cagcgcattc cctgcaccga gatcctcgat cagcgtgtgc tttccggggc
      361 ccgtggccgg cacggcgggc tcgcgacccg accccgcggc agcttgcgat tcacgtgaac
      421 ggtcgtgcga cgaggggatc gacatggcaa tctcggccat agtacgtacg cgccgacgcg
      481 gatcttgaca tcgagctgat gacagctgcg gcgcgcaggc tcggacacga gtccccatcg
      541 ggtgccagac gtcagctggc ggaaaacgca aggtatttcc aagcgttgtc atggctcatc
      601 gcacacccag gtggatcgcg atccgggcga catggcaccg cgatggcccc cctacaccct
      661 ccgcatcact tgcgttaggc tctcgccgcg atggcctggt cgtcgctccc cggtgcgagg
      721 cgaccgaaag gaagtgcgct ggcatgaagg catctttcag ggcattgatc gtcacccaga
      781 cgacggacac gttcgcagtg ggagtccggg agatgcggcc cgaggagctg cccgcggggg
      841 aggtattgat ccgcgttgcg tactcggctg tgaactacaa agatgccctg gtctgcacgc
      901 aaaatggcag ggtcgcacag acctctccgc tggtgcccgg tatcgaattg acaggcgggt
      961 cgtcgagtca agcgactcgc gttttcgagc cggcgatcgg gtcattgcca atgacttcgg
     1021 ccacagaatc ggcgtttctc agcatggtgg attcagtgaa tatgcgcgtg tacccggcga
     1081 tttcgtgagc gcgctcgccc cggagctcgg cttcccgcaa gcgctcgtgc tctccggggg
     1141 gctgacagcg atgatgagcc tgagcgagct cgaaaaggcc gggctgaccc cgcagaacgg
     1201 cccggtcctg gtcaccggag ccaccggtgg ggtggggagc ctcgccatcg gcctcttgtc
     1261 acagcgtggc tatacggtgg ccgccagcac gggcaaaccc gacgccgctg actacttgag
     1321 gcgactggga gcgagcgaga tcctgaaccg agcagagacg tccgccgaat cgtcacggcc
     1381 actggaagcc gagcgctggg ccgggagcat cgactcggtc ggtggtgcaa cgctggccta
     1441 tctgatgcgc acgacgaagc ggggaggagc gatcgcggcc gtcggcgtcg ccggaggggc
     1501 agcctttcat ggcaccgtat ttccactcat cctgaggggg atcaaggtgc tgggcatcga
     1561 gatgccctcg acgcccctgg caacgcgccg gcaactgtgg gaacagctgc agaaagaagc
     1621 agggctcctg agccttgccg actccatcct ccgcgaggtg gccctggacg acgttccgag
     1681 ggtctcgcag gcgatgttgc gcgggcagag gcaggggcgc atcctggtca gactcggcga
     1741 gccagagagc acctgaacca gagacaggcg gtcccgcgac acgcgtgcag agcggtcgtc
     1801 ggggcctgcc atgtcgatca ccggctgcgc gaccagggcg ccgcgatggg caccgaggca
     1861 gcgcggttcc tcctggtgtg gatggccggc gccccgccgt gcgcgatgcc gcgccggtca
     1921 cgctggatca gtgctcgaac tccgagaccg gaaccttgat cacgagcgga ccgagaccgg
     1981 cggccgtgga cgtcaagggc gggttcagca cgccggcggc gatggcggca ttgagcccgc
     2041 tctcggagcg cggcgggacg aacgtcgccg cgttgaagtt cggccccggg aacaccagga
     2101 acccgtcgaa ggtccggatg ccttcctcct gcgtgatgat gtgatagtgg tcgaagttgg
     2161 gctggccgtc gacgtggtgg gtcgcgcctg ccggtccctg cgcgaacacc gcgaacagct
     2221 tgccgtgggt gctcgacgga tagggggcgt tatgctggat ggcggttccg agattccaga
     2281 tgaagaatgt ctcggcctgg gccgtgaaga agcccgggat ctggaacgtg aacgtccggt
     2341 cgcggaagaa cgcatactcc tcgtcctcgg ggatcggcgg cagggtcgtc gtcgaccggg
     2401 tatttgccgg gtcgacggcg ctcgtcgacg gatccgagga cggttgcgag gagtcgctgc
     2461 ccacgacgca tccacccagc agcgcggacc ccacgagcgc cgtgagcagc gccttcacgt
     2521 tggaacgatg ttggatcttg aaaatatccc acattctcat accctttctt tgaacagatt
     2581 cgggctcagg tacgggctcg gggggcctct gaacgtttca tgccacccag atacaacccc
     2641 tgaaattcgc tacttgggct gtctctgctt gcgcagattt gcgcaacgtc gcgcaatctg
     2701 cccgacgttg cgcatccggg cgttgcgcac gtccgccgag actgcgttgc acaaactcgc
     2761 cgccgccgcg gcgggatgcg cgacgttctt cgccgcggcg cgtgccggga cgcgatcgat
     2821 cccgacgtca agcggtatcc gtcacacggc gacgggcggc ggcgccgaga acgtgcggcc
     2881 ggccgggatg ccgcgcgtga tatcctcggt gagagtgggc cccttggacg acaatgacga
     2941 gctggggctc accgctgtca cggccgtggt accgggcgac gccgtcgacg agagcccgcc
     3001 cggtcacctg cagctgttcg tgatcagcgg cgggacgttc gccgcgcacc ccttgaagga
     3061 ggccgggacc gccacgatcg gccggggccg ccagtgcgac atcgaggtcg atgatcgatc
     3121 gatctcacgg cggcacgcga tcctgagcgt cggcgagacc gtcacgatcg aggatctcgg
     3181 cagcgccaac ggtacgttcg tgcgcggaga gcggatcgcg ctcggcagcc cggcggtgat
     3241 ctcggtgggc gagctggtcg tcctgggcag cgccaacatc atcctgcagc gccgcgcgca
     3301 gccggtgccg ccgcgcaagg tgtggagccg cgagtcgttc gagctcttgc tcgaggaaga
     3361 gtgcgcgcgc gaggcgcggt acggcgccga gtttgcgctg ctgtgcatcc acgccgatcg
     3421 ccgcgcaccg ccgggcttcg tcgaggacac catcgccgag ctgctgcgcg aatcggacat
     3481 cgtggcccgc tacggcccgc acgaatacga ggtgctgctg cgcgacacgc cggccgcgaa
     3541 caccgacggc gccgcgcgcc ggatcgacag caagctgctc gagcgcgggc tccggtgcca
     3601 gatctcggtc gcgtgctgcc cgcgcgacgg caggaccgcc tcccagctca gcgccaaggc
     3661 gcaggggccg gtggtcaagg acagggccac cggcgcgagg gccgacgtcg tggtctccga
     3721 tccgcggatg cggggcctgc accgcctggt cgaccaggtc gccggttcca gcatcagcgt
     3781 gctgctgctc ggcgagaccg gggtcggcaa ggaggtgttc gcgcgcgccg tgcaccgcgc
     3841 ctcgccgcgc gcggccggtc cgttcgtcga ggtcaactgc gctgcgctca ccgagaccct
     3901 gctcgagagc gagctgttcg gccacgagcg gggcgcgttc accagcgccg tgtcggccaa
     3961 gccagggctg ctcgagatgg ccgatggcgg taccctgctg ctcgacgaga tcggcgacat
     4021 gccgctcgcc acccaggtca agctgctgcg ggtcatcgag gactcgcagc tgcgccgggt
     4081 cggcgggctc aagccccggc acatcgacgt ccggttcgtc gcggcgacca acagcgacat
     4141 cgaagcccag gtcgagcagg ggacgtttcg tcgcgatctg ttcttccgcc tgaacggcgt
     4201 cacgatcctg atcccgccgc tccgcgagcg gctcgccgag atcgagccgc tggcccgggc
     4261 gttcatccgc ggctgcagct caccgggcgg cgggccgcgc cccgagctcg cgcccgaggc
     4321 gctcgagtgg ctccggcatc acccctggcc gggcaacgtg cgcgagctca agaacgtgat
     4381 cgaacgcgcg gtgctgctgt gcggcagcgg cccgatcctc cccgagcacc tgtcgacccg
     4441 caggccgacc gtgcagacca ccgccgacga ggcgacgacg cgcccgagcc cgtggcctcg
     4501 ggatgcgcgg ccgcacgctg ccacggacgt cgacgtgcgc atcccgatgt tcgcggcgcg
     4561 cccccgcaag ggctcccagg aggaggcgca gtggatcatg cagacgctcg agcgagccgg
     4621 cggaaaccag accgtcgcag cgcgcctgct cggcatctcg cgccgcaccc tcgtcaaccg
     4681 gttgaacgat tacgagcacg tccaccggcc gcgcaagaag ccgaaggcgc acccctccga
     4741 gtgatcgccg gcgcgacctc ggcgtcgcga gatgtgcacg aggtctggtc gtcgtgtggc
     4801 acggagggcg ggtgtgccgc gcgatgtgcc accggccggc ggcgatgcgg cgtggctcga
     4861 cggatggttc gcgatcgcgc gcggcgcccg ggcggcggca cggcggctgc agtcggtagc
     4921 gtgcagcggt ccgacgatgt gccgaatggg tcggagcagg tcggtcgcga ttgccacgtg
     4981 ggagacaggg acatgaacaa gaagacgaag aagagcgaca agggtgggct gaccttcaag
     5041 cgcgagacga tccggagtct gaccgacgcc gagatcgggc tcgtcgcggg cggccagcgc
     5101 tgcgcggcgg cggactcgtg cgtgggcgtc acctgcccga ac
//
LOCUS       DASVOR010001402         3873 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_276568,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001402 DASVOR010000000
VERSION     DASVOR010001402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276568"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="VF516_26675"
     CDS             complement(<1..716)
                     /locus_tag="VF516_26675"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEX8111354.1"
                     /translation="MDAPAAASQPPSPAALSPAQIARFAAIVGERNCIWRPDELRTYE
                     SDALTTFHAMPGVVVLPASRAEVVEVVKLAHRERLPIVPRGSGTGLSGGALPVAGGVL
                     LGLSRMKRIVEIDFENQWMRVEPGVINLEISRRIAPRGYYYAPDPSSQQVCTIGGNVA
                     ENSGGAHCLKYGFTTNHVLGATVVLPDGEIVELGGPVADPIGYDLRGALVGSEGTLGV
                     VVEVVLRILRRPEATRTFFAT"
     gene            complement(729..2300)
                     /gene="aceB"
                     /locus_tag="VF516_26680"
     CDS             complement(729..2300)
                     /gene="aceB"
                     /locus_tag="VF516_26680"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEX8111355.1"
                     /translation="MPTIDITGPITSDGRRVFTAEALRFIGDLEAKFGPVRTSLLEAR
                     RKRDAEIKAGASYGLLAGTEAVRRGDWKVAPTPPDLQRRHVEITGPVEPKMLINALNS
                     GADIFMADFEDSLSPTWDNVVTGQAGLIDAVRRTLAFTSPEGKKYTLGDKLATLIIRP
                     RGWHLTEKHAAFEGRPVSASLFDFGLYFFHNVRALIERGTGPYFYLPKLESHREARLW
                     NDVFVAAQDALGVPRGTIRATVLVETLPAAFEMDEILYELRDHASGLNAGRWDYLFSA
                     IKKLRDRPGLAIPDRGQLLMTVSFMRAYTERLVRTCHHRGAHAMGGMAPFIPSRKNPE
                     INAAALAKVRDDKLREVKDGFDGTWVAHPDLVPTAAEVFNGALGDKPHQKDRLRDDVQ
                     VTDAELLDLGVPGGKITEAGVRNNISVALQYLSAWLAGSGAVAIFNLMEDAATAEISR
                     AQLWFWVQHGGVLDDGRRVDAALYRALRDDEVAKLAGLPAEAQLPAAKALLDRLVEGS
                     GFLDFLTSPAYEQLG"
     gene            complement(2492..>3873)
                     /locus_tag="VF516_26685"
     CDS             complement(2492..>3873)
                     /locus_tag="VF516_26685"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8111356.1"
                     /translation="VQTYVSVSTPRVVLTHIRVIDGAGHAPLDDRNVVIDHGKIAAID
                     AGADVAAADGTTVLDGRGRSVMPGIVGMHDHLFYIARPNYDEEWSWEQPLIAPEMMFS
                     APRLYLAAGVTTARTTGSMEPYADLNLKREIDAGKLVGPHLDVTGPYLEGTPSMFIQM
                     HHLTSPEDARQFVNYWADRGVTSFKAYIDITRAELKAAIEAAHKRGIKVTGHLCSVSY
                     DEAADLGIDDLEHGFYVNTQLDPGKKPDVCPSTVGLPTLMKMTPDSPEARALIAKLVK
                     HRVAITSTLPVFESGVPGRPPLRQQLLDTMTPEARQAYLYARNRPASLPPRPFDPLEA
                     LQHTMALERAFVAAGGLLIAGPDPTGNGGVVAGFGDQREIELLVEAGFSPVEAIQIAT
                     LNGAKYLGRDDHIGSIAVGKNADLVVVQGDPATRIADVENVEVVFKDGVGFDPAKLLA
                     SVKARYGQY"
BASE COUNT          533 a         1296 c         1460 g          584 t
ORIGIN      
        1 gtcgcgaaga acgtgcgggt cgcctcgggg cggcgcagga tgcgcagcac gacctcgacg
       61 acgacgccga gcgtgccctc gctgccgacc agcgcgccgc gcaggtcgta gccgatcgga
      121 tcggcgaccg ggccgccgag ctcgacgatc tcgccgtcgg gcagcaccac ggtggcgccg
      181 agcacgtggt tggtggtgaa gccgtacttg aggcagtggg cgccgccgga gttctcggcg
      241 acgttgccgc cgatcgtgca gacctgctgc gacgacgggt cgggagcgta gtagtagccg
      301 cgcggcgcga tccgccggga gatctcgagg ttgatgacgc ccggctcgac gcgcatccac
      361 tggttctcga agtcgatctc gacgatgcgc ttcatccgcg acaggccgag caggacgccg
      421 ccggcgaccg gcagcgcgcc gccggacagc ccggtgccgg agccgcgcgg gacgatcggc
      481 aggcgctcgc ggtgggcgag cttgacgacc tcgacgacct cggcgcgcga cgccggcagg
      541 acgacgacgc cgggcatggc gtggaacgtg gtgagcgcgt cggactcgta ggtgcgcagc
      601 tcgtcgggcc gccagatgca gttgcgctcg ccgacgatcg cggcgaaccg cgcgatctgg
      661 gccggcgaca gggccgccgg agacggaggt tgcgaggccg cggccggggc gtccatgtgg
      721 gcgctccgct accccagctg ctcgtacgcc gggctggtga ggaagtcgag gaacccgctg
      781 ccctcgacca gccggtcgag cagggccttg gcggcgggca gctgggcctc ggcgggcagg
      841 ccggcgagct ttgcgacctc gtcgtcgcgc agcgcgcggt acagcgcggc gtcgacccgg
      901 cgcccgtcgt cgagcacccc gccgtgctgg acccagaacc acagctgggc ccgcgagatc
      961 tcggcggtcg ccgcgtcctc catgaggttg aagatcgcga ccgcgccgct gccggcgagc
     1021 cacgccgaca ggtactggag cgcgacgctg atgttgttgc ggacgccggc ctcggtgatc
     1081 ttgccgccgg ggacgccgag atcgagtagc tcggcgtcgg tgacctggac gtcgtcgcgc
     1141 agccggtcct tctggtgcgg cttgtcgccg agcgccccgt tgaatacctc ggccgccgtc
     1201 ggcacgaggt cggggtgggc gacccaggtg ccgtcgaagc cgtccttgac ctcgcgcagc
     1261 ttgtcgtcgc gcaccttggc gagcgcggcg gcgttgatct cggggttctt gcggctcggg
     1321 atgaacggcg ccatgccgcc catcgcgtgc gcgccgcggt ggtggcaggt ccggaccagc
     1381 cgctcggtgt acgcgcgcat gaacgacacg gtcatcagca gctggccgcg atccggaatg
     1441 gccaggccgg gccggtcgcg cagcttcttg atcgcgctga acaggtagtc ccagcgcccg
     1501 gcgttgaggc cgctggcgtg atcgcgcagc tcgtacagga tctcgtccat ctcgaacgcg
     1561 gccggcaggg tctcgacgag gacggtggcg cggatcgtgc cgcgcggcac gccgagcgcg
     1621 tcctgcgccg cgacgaacac gtcgttccac agccgggcct cgcggtgact ctccagcttg
     1681 ggcagataga agtacgggcc ggtgccgcgc tcgatcagcg cgcggacgtt gtggaagaag
     1741 tacaggccga agtcgaacag gctcgccgac accggccggc cctcgaacgc ggcgtgcttc
     1801 tcggtcaggt gccagccgcg cgggcggatg atcagggtcg cgagcttgtc gccgagcgtg
     1861 tacttcttgc cctcgggcga ggtgaaggcc agcgtccgcc gcacggcgtc gatcagcccg
     1921 gcctggccgg tgaccacgtt gtcccaggtc ggggacagcg agtcctcgaa gtcggccatg
     1981 aagatatccg cgcccgagtt gagcgcgttg atcagcatct tgggctcgac cggcccggtg
     2041 atctcgacat ggcggcgctg gaggtccggc ggggtcggcg cgaccttcca gtcgccgcgg
     2101 cgcaccgcct cggtcccggc gagcaggccg tacgaggcgc ccgccttgat ctcggcgtcg
     2161 cgcttgcgcc gcgcctcgag caggctggtg cggaccgggc cgaacttcgc ttccaggtcg
     2221 ccgatgaagc ggagcgcctc ggcggtgaac acgcgacggc catcggatgt gatcggacct
     2281 gtgatgtcga tcgtgggcat ctgctgactc ctgcgcagcg atcgccgcga ctgcttgcgg
     2341 cggatggtac ctggaaaccg cccgacgcca agccctcggc gatgcaacgc tgcgtgcaca
     2401 agcgatggcg tgcgccggtg cggggtgtcg caaatccgac ggtgggcgga acgctcaccc
     2461 gggaaactga gcagcggtcg gcccggcgcg gtcagtactg cccgtagcgc gccttgaccg
     2521 acgcgagcag cttggccggg tcgaagccga cgccgtcctt gaacacgacc tcgacgttct
     2581 cgacgtcggc gatccgggtc gcgggatcgc cctggacgac gaccaggtcg gcgttcttgc
     2641 cgaccgcgat gctgccgata tggtcgtcgc ggcccaggta cttcgcgccg ttgagcgtgg
     2701 cgatctggat cgcctcgacc ggcgagaacc cggcctcgac caggagctcg atctcgcgct
     2761 ggtcgccgaa cccggccacg acgccgccgt tgccggtcgg atcagggccg gcgatcaaga
     2821 ggccgccggc ggcgacgaac gcgcgctcga gcgccatggt gtgctgcagc gcctcgagcg
     2881 ggtcgaacgg gcggggcggc agcgaggccg ggcggttgcg ggcatagagg taggcctggc
     2941 gggcctcggg ggtcatcgtg tcgagcagct gctggcgcag cggcggccgg ccgggcacgc
     3001 cggactcgaa caccggcagg gtcgacgtga tcgcgacgcg gtgcttgacc agcttggcga
     3061 tcagcgccct ggcctccggg ctgtccggcg tcatcttcat cagcgtcggc agcccgacgg
     3121 tcgacgggca gacgtcgggc ttcttccccg ggtcgagctg ggtgttgacg tagaacccgt
     3181 gctcgaggtc gtcgatgccg agatcggcgg cctcgtcgta gctcaccgag cacaggtggc
     3241 cggtgacctt gatgccgcgc ttgtgcgccg cctcgatcgc cgccttgagc tcggcgcgcg
     3301 tgatgtcgat gtaggccttg aacgacgtca cgccgcggtc ggcccagtag ttgacgaact
     3361 ggcgcgcgtc ctcggggctg gtcaggtggt gcatctggat gaacatgctc ggcgtgccct
     3421 cgaggtacgg gccggtgacg tcgaggtggg ggccgaccag cttgccggcg tcgatctcgc
     3481 gcttgaggtt gaggtcggcg tagggctcca tgctgccggt cgtgcgcgcg gtggtgacgc
     3541 cggcggcgag gtagagccgg ggcgccgaga acatcatctc gggcgcgatc agcggctgct
     3601 cccagctcca ctcctcgtcg tagttcgggc gcgcgatgta gaacaggtga tcgtgcatcc
     3661 cgacgatgcc gggcatcacc gaccggccgc ggccgtcgag caccgtcgtg ccatcggccg
     3721 cggcgacgtc ggcgcccgcg tcgatcgcgg cgatcttgcc gtggtcgatc acgacgttcc
     3781 ggtcgtcgag cggcgcgtgc ccggcgccgt cgatcacacg gatgtgggtg agcacgaccc
     3841 gcggcgtcga caccgagacg taggtctgca ccg
//
LOCUS       DASVOR010001403         1762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393705,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001403 DASVOR010000000
VERSION     DASVOR010001403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1762
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393705"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..967)
                     /locus_tag="VF516_26690"
     CDS             complement(23..967)
                     /locus_tag="VF516_26690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010918528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8111357.1"
                     /translation="MANIICGVDVSSQALDAEIWPGGAQLQVKNTPQGVAELAAWCKA
                     QGAELAVMEATGGYERQAFRLLWAHGVAAAVVNPRHVRRFAEAMGVLEKTDRLDAGVI
                     GRYAAARGVKPEAPPPAHQERLAALVTRLGQLTALKVAQENQRRLVEDATVQACFAEI
                     LRVVNGQIRGFEAEIAELLQADPLWRELDRVFRQTKGVADRTVARLMAEMPEIGTLTG
                     KEVAKLAGLAPLANDSGKHIGRRHVRGGRTAIRSILYLVAGVVARHEPDYIAFRQKLL
                     DAGKPRKVVRIALARKLLVRLNAKARDVRTALNMLAAA"
     gene            1214..1585
                     /locus_tag="VF516_26695"
     CDS             1214..1585
                     /locus_tag="VF516_26695"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEX8111358.1"
                     /translation="MAYRISNRMRNRAGTLRKSMTEPEVMLWSRLKTRGTDKPIFRRQ
                     YVYESMVLDFYCPAARLAIEVDGSTHWDEAKRAKDAARDRWLASRGVAVLRVGAGHVY
                     GDLGGVADIVIQRADERIRGP"
BASE COUNT          266 a          602 c          620 g          274 t
ORIGIN      
        1 gggagagcga ctgtctggca ggtcaagcgg cggcgagcat gttgagggcg gtacggacgt
       61 ccctggcctt ggcgttgagg cggacgagga gtttgcgggc gagggcgatg cggacgacct
      121 tgcggggctt tccggcgtcg aggagtttct ggcggaaggc gatataatcg ggttcgtggc
      181 gggcgacgac gccggcgacg aggtagagga tgctgcggat ggcggtgcgg ccgccgcgga
      241 cgtgacggcg cccgatgtgc ttgccggaat cgttggcgag cggagccagg ccggcgagct
      301 tggcgacctc cttgccggtg agggttccga tttcgggcat ctcagccatc aggcgggcga
      361 cggtgcggtc ggcgacgccc ttggtctggc ggaagacccg gtcgagctcg cgccacagcg
      421 gatcggcctg gagcagttcg gcgatctcgg cctcgaagcc gcggatctgg ccgttcacga
      481 cgcgcaggat ctcggcgaag caagcttgca ccgtcgcgtc ctcgaccagc cggcgctggt
      541 tctcctgggc gaccttgagc gcggtgagct ggcccagccg ggtcaccaag gccgccagcc
      601 gctcctggtg ggccggcggc ggcgcctcgg gctttacgcc cctggccgcc gcgtagcggc
      661 cgatcacgcc ggcgtcgaga cggtcggtct tctccagcac gcccatcgcc tcggcgaagc
      721 ggcgcacatg gcgcgggttg accaccgcgg ccgccacgcc atgcgcccac agcagccgga
      781 acgcctgccg ctcatagccg ccggtggcct ccatcaccgc cagctccgcc ccctgggcct
      841 tgcaccaggc ggccagctcg gccacgccct gcggcgtgtt cttcacttgc agttgcgccc
      901 cgccgggcca gatctcggcg tccagcgcct ggctggacac gtccactcca cagataatgt
      961 tcgccacggt aacctgcctt gcgcatgcga tcgatgttca agcgactgtt cggtcgtgcg
     1021 tgaccgcggc ggcggccctg gtctctccca cggtgaaagc ctgaggagaa cacgggcgca
     1081 agccgccgca cgggagggcc ggtggccacc ggccctcccg caacgccttc gccatcacgc
     1141 ttcgacgcta ccgtccagat acaaggggga ccgcgccccg aagagggcgg ggtggagagg
     1201 gcaggcagcc gacatggcct atcggatttc caaccgcatg cggaaccgcg ccgggaccct
     1261 ccgcaaatcc atgaccgagc cagaggtcat gctctggtca cgcctcaaga cccgcggaac
     1321 cgacaagccg atcttccgcc gccagtatgt ctatgaatcg atggtgctcg acttctactg
     1381 cccagcggcc aggctcgcga tcgaggtcga tggctccacc cattgggatg aggcgaagcg
     1441 ggcgaaggac gcggcgcgag accgttggct tgcgtctcgg ggcgtagccg tgctgcgggt
     1501 gggcgccggg catgtctacg gcgacctggg cggggtggcc gacatcgtga tccagcgggc
     1561 cgacgagcgg attcgcggac cgtaagtcac aggcgaccgc atgccctctc caccacccgc
     1621 tcttcggcgg gcggtccccc tctcccactg cgtgggaaag gaacgcccct tcctcgcgtc
     1681 gcaccttgca ggttacagct tgccgatgga ttcccgtccc gtcgacgccc tcaccgaagc
     1741 cgaagccgcc gatgagctga cg
//
LOCUS       DASVOR010001404         1894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_72648,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001404 DASVOR010000000
VERSION     DASVOR010001404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1894
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72648"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VF516_26700"
     CDS             <1..949
                     /locus_tag="VF516_26700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111359.1"
                     /translation="EVVAEALQKGGAMQTASVPTLASAPTLGEQASLASEIAAHGAAP
                     AADPALAAALADTTEKWLIGKNRMDYGPFSLADVIAQIERGEIVAGNQIQDKDTGARA
                     SVEDHPLLGPLVEAARQKRDDARRAQAEIQQQSRDKKRGAMLYALIALGVVVVAVAVY
                     LVLRSVGRDDGGPQVAGVSALDGASLKVKVSEPKKPAATHRRAAGKRPAGPASGGQNL
                     TLDLSDDSDETETLEMSAVYGVYSRYGRGLGGCLQSTGERSAAIGIIIDGPTGRVTWV
                     KVNDAQSGALYSCLSGVLRTMQFPKIHGPRTRAEFEISI"
     gene            953..>1894
                     /locus_tag="VF516_26705"
     CDS             953..>1894
                     /locus_tag="VF516_26705"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111360.1"
                     /translation="MPDSSSRSPSFPPRRSRRSDDPDGGNRAGLAKVIPRRPAVSWWL
                     GLVLALGFAALVTPAITAEHWLLRFGPDTIAEHREAPYTVRAPMTGSGELRVGGGVVV
                     ARGEIATRDEAALADAITSATPGGSGLYLAFFGLTAVLGALFTHHVGRSTKGRLVRVQ
                     VVSLATIALLAVAVKIVLLSTALSALVVPIAVLAMVPTMVLDRVVGLATGVLAALVVS
                     LQAPFDIGLALLLLVQTAAAGLVIPERPRRRWFAALTAGAVTTLCTAATYLLLTYLIT
                     GHTPELRDPLHSPWLAAAVGPAAAALLAVPVLPIYQLL"
BASE COUNT          234 a          724 c          679 g          257 t
ORIGIN      
        1 cgaggtcgtc gccgaggcgc tgcagaaggg cggcgcgatg cagaccgcgt cggtgccgac
       61 cctggcgagc gcgcccacgc tcggcgagca ggcgtcgctg gcgtccgaga tcgccgccca
      121 tggcgcggcc ccggccgccg accccgcgct cgccgccgcg ctcgccgaca ccaccgagaa
      181 gtggctgatc ggcaagaacc ggatggacta cggcccgttc tcgctcgccg acgtgatcgc
      241 ccagatcgag cgcggcgaga tcgtcgccgg caaccagatc caggacaagg acaccggcgc
      301 gcgggccagc gtcgaggatc acccgctgct cgggccgctc gtcgaggccg cgcggcagaa
      361 gcgcgacgac gcgcggcgcg cccaggccga gatccagcag cagagccgcg ataaaaagcg
      421 cggcgcgatg ctgtatgcgc tgatcgcgct cggtgtggtc gtggtcgcgg tcgcggtgta
      481 cctcgtgctc cgctcggtcg gccgcgacga cggcggcccg caggtcgccg gggtcagcgc
      541 gctcgacggc gcgtcgctca aggtcaaggt cagcgagccc aagaagccgg ccgcgaccca
      601 ccgtcgcgcc gccggcaagc gtcccgccgg cccggccagc ggcggccaga acctcacgct
      661 cgacctgtcc gacgacagcg acgagaccga gaccctcgag atgagcgcgg tctacggcgt
      721 ctactcgcgc tacggccgcg ggctcggcgg ctgcctgcag tcgaccggcg agcgcagcgc
      781 ggcgatcggc atcatcatcg acggtccgac cggccgggtc acctgggtca aggtcaacga
      841 cgcccagtcc ggtgcgctgt acagctgtct gtccggcgtg ctgcgcacca tgcagttccc
      901 gaagatccac ggcccgcgga cccgcgccga gttcgagatc tcgatctagc ccatgccgga
      961 ctcctcctcc cgcagcccgt cgttcccgcc gcgccgctcg cgccggtccg acgaccccga
     1021 cggcggcaat cgcgccggcc tcgccaaggt gatcccgcgc cggcccgcgg tgtcgtggtg
     1081 gctcggcctg gtgctcgcgc tggggttcgc cgccctggtg accccggcga tcaccgccga
     1141 gcactggctg ttgcggttcg gccccgacac gatcgccgag caccgcgagg cgccgtacac
     1201 cgtgcgcgcg ccgatgaccg gcagcggcga gctccgggtc ggcggcgggg tcgtggtggc
     1261 gcgcggcgag atcgcgaccc gcgacgaggc ggcgctcgcc gatgcgatca cctcggccac
     1321 ccccggcggc tccgggctgt acctggcgtt cttcgggctc accgccgtgc tcggcgcgct
     1381 gttcacccac cacgtcgggc ggtcgaccaa gggccggctg gtgcgggtcc aggtcgtcag
     1441 cctggcgacc atcgcgctgc tcgcggtcgc cgtgaagatc gtcctgctgt cgacggcgct
     1501 gtcggcgctg gtcgtgccga tcgccgtgct cgcgatggtg ccgaccatgg tgctcgatcg
     1561 cgtcgtcggg ctcgccaccg gcgtgctcgc cgcgctggtg gtcagcctcc aggcgccgtt
     1621 cgacatcggg ctcgcgctgc tgctgctggt ccagaccgcg gccgccgggc tggtgatccc
     1681 ggagcggccg cggcggcgct ggttcgcggc gctgaccgcc ggcgcggtga ccacgctgtg
     1741 caccgcggcg acctacctgt tgctcaccta cctgatcacc ggccacaccc ccgagctgcg
     1801 cgatccgctg cactcgccat ggctcgcagc ggcggtcgga cccgcggcgg ccgcgctgct
     1861 cgccgtgccg gtgctcccga tctaccagct gctg
//
LOCUS       DASVOR010001405         6117 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_335604,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001405 DASVOR010000000
VERSION     DASVOR010001405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6117)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6117)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6117
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335604"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..894)
                     /locus_tag="VF516_26710"
     CDS             complement(136..894)
                     /locus_tag="VF516_26710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111361.1"
                     /translation="MRTLAVVVLWIGVVHAEPDASTSELLRQGNTAALAGDWSHVSQL
                     IEPLLLHPLAAADLAEAHRLAGLAALFQQHGDVAERHFLAYLRIAPTGRLDPALYPPE
                     AVTFFSDVASRHAAELGSRPSPSRSWWLTLIPPFGQLQNEERTKAYALGGVLGTLLVA
                     DLTSYYFLSKWCAVTDGPKGGGLSCYNGSDHNHEAARLRPYNIASGIGVIAVYAYGVV
                     DGILGYRRRSRELAVQPFVAAAPDRGTLGIIARF"
     gene            complement(906..1403)
                     /locus_tag="VF516_26715"
     CDS             complement(906..1403)
                     /locus_tag="VF516_26715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111362.1"
                     /translation="MRLRARARLVAGAGVVLAGCLYTEPINRQPKVFPPQRLCDAAHP
                     ADPCSPDNLHRGDHVMVKAVFHDPDGDPNDGTVQWRISACDGPDATCDVNRLYDQMVP
                     AQPPYPEFTVPAVLTGTEVPTRNIVFEITVFDDRGASSTGEDSWNVIPDAPAAAQEGS
                     GAGTP"
     gene            complement(1499..3682)
                     /gene="katG"
                     /locus_tag="VF516_26720"
     CDS             complement(1499..3682)
                     /gene="katG"
                     /locus_tag="VF516_26720"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF011635.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEX8111363.1"
                     /translation="MSTEAKCPFKHQAGSGTSNRDWWPNQLRLETLHQRSSLSNPMGE
                     DFNYAKEFKSLDFAALKKDLLALMTDSQDWWPADFGHYGPLFIRMAWHAAGTYRIGDG
                     RGGAGSGTQRFAPENSWPDNANLDKARRLLWPIKQRYGRKISWADLFILTGNVALESM
                     GFKTFGFGGGRADIWEPQGDVYWGSETQWLADKRYSGDRELEGPLGAVQMGLIYVNPQ
                     GPNGNPDPIAAARDIRETFRRMAMNDEETVALIAGGHTFGKTHGAGEEAKHIAREPEG
                     AAIDEQGFGWRNSLGKGHGGHTITSGLEGTWTTTPTKWSNNFFENLFGYEWELFKSPA
                     GANQWRPKNGGGAGTVPDAHDPAKRHAPTMLTTDLSLRLDPAYEKISRRFMQHPDQFA
                     DAFARAWFKLTHRDMGPRARYLGPEVPKEELIWQDPVPAADHPLIDERDAATLKANIL
                     AAGLTVSQLVSTAWASASTFRGSDKRGGANGARIRLAPQKDWAANQPAQLAKVLGALE
                     KVQKEFNGAQSGGKKVSLADLIVLGGCAGIEQAARNAGQTVTVPFKPGRTDASQAQTD
                     AESFAVLEPVVDGFRNYQKERYSVPSEELLVDRAQLLTLTAPEMTVLIGGLRVLGTNV
                     GPSKQGVFTKRPEALTNDFFVNLLDMGTAWKPVSDAQEVFEGRDRKTGEVKWTGTRVD
                     LVFGSNSVLRALAEVYASSDAQNKFVQDFVAAWTKVMNLDRFDVA"
     gene            complement(3777..3917)
                     /locus_tag="VF516_26725"
     CDS             complement(3777..3917)
                     /locus_tag="VF516_26725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111364.1"
                     /translation="MTGASPNTELLERCVVLDAQGFVKTRGARRVSDLAGSRWRGAAR
                     FA"
     gene            complement(3914..4279)
                     /locus_tag="VF516_26730"
     CDS             complement(3914..4279)
                     /locus_tag="VF516_26730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111365.1"
                     /translation="MPRPAFVGVSAADGENSCGAPALSVVLEAIAPGGQAGTSSKIEN
                     HLGFPVRAQGEGVRHGTSERPVQQMSRNRRIAETPNLQLLVETEIVELGGDAALERVT
                     WLDRRTGERAGHAIGHVVM"
     gene            4351..5751
                     /locus_tag="VF516_26735"
     CDS             4351..5751
                     /locus_tag="VF516_26735"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8111366.1"
                     /translation="MAVLDRDRAQISLLHLAWPMFVENVLRASLMSVDTAMLSRYSEN
                     AVAAMSLVNQFAFFIQLLYMTVSTGASILITQQLGAGRPREARLIAVGSLTLVVAFSL
                     VVSSVFTLVTGPLIDLYGLDPEVARYARQFLVLYGGLSFFMAVNIAQASILRAWGHTR
                     DPMLVNVLSLVLTVLGNALCLFGPFGFPVLGVTGVAASTVLSQVVACALCFVAMRRRR
                     ELELPLGEAARIPGRLYSAILAIGIPTAGENLAYNLSQIAIMAMLSALGAVPLATYGI
                     VMAVLRYVFMAGVSIGSAAQLKVGYLVGSGRHAEAKQRVYRYFALASVCTISSVLLLE
                     LFHGSVLGLFSTDRDILALASTVLLVAVFHEPGRNFNTVFIPALKGAGDVRFPVTMGI
                     LSMWGIGVAGAWLLGIRLHWGLPGIWIAMAADEWLRGLVMLRRWRSGVWQASGRLEAA
                     GADVAEVAAVEIEEGI"
BASE COUNT          814 a         2140 c         2154 g         1009 t
ORIGIN      
        1 gctggcggtt ccgcgacgcg gcggcattcg cactggcggc cgaggcgccg ccgccgtccc
       61 ggatcgcgcg cgcggtcgaa ggcatcgtgc ggcgcgccgg cccacacacg cacccgcgcg
      121 cgtgatcacg acgcgtcaaa accgggcgat gatccccagc gtgccgcggt ccggcgccgc
      181 ggcgacgaac ggctggaccg ccagctcgcg ggatcgccgg cggtatccga ggatcccgtc
      241 gaccacgccg tacgcgtaca ccgcgatgac cccgatcccg ctcgcgatgt tgtacggccg
      301 gagccgcgcg gcctcgtggt tgtgatcgct gccgttgtag cagctcagtc cgccgccctt
      361 cgggccatcg gtcaccgcgc accacttgct caggaagtag tagctggtga gatcggcgac
      421 gaggagcgtg ccgagcacgc ccccgagcgc gtaggccttg gtccgctcct cgttctggag
      481 ctgaccgaac ggcgggatca gggtcagcca ccaggatcgc gacggcgatg gccgtgaccc
      541 gagctcggcg gcgtggcgcg acgcgacgtc gctgaagaac gtcacggcct cgggcgggta
      601 cagcgccgga tcgagccggc cggtgggcgc gatgcgcagg tacgccagga agtggcgctc
      661 ggcgacgtcg ccgtgctgct ggaacagcgc ggccaggccg gccagccggt gggcctccgc
      721 gaggtcggcg gccgcgagcg ggtgcagcag cagcggctcg atcagctgcg acacgtgcga
      781 ccagtcgccc gcgagggccg cggtgttgcc ctggcgcagc agctcggacg tggacgcgtc
      841 gggctcggcg tgcacgacgc cgatccacag cacgaccacg gcgagcgtgc gcacggcgcc
      901 ggtcctcacg gcgtgccggc ccccgatccc tcctgcgcgg ccgcgggcgc gtccgggatc
      961 acgttccacg aatcctcgcc ggtggacgac gcgccgcggt cgtcgaacac tgtgatctcg
     1021 aacacgatat tccgcgtcgg cacctcggtg ccggtcagca cggccggcac cgtgaactcc
     1081 gggtacgggg gctgcgccgg gaccatctgg tcatacagcc ggttcacatc gcaggtggcg
     1141 tcgggtccgt cgcacgccga gatcctccac tgcaccgtgc catcgttcgg atcgccgtcg
     1201 gggtcatgga agacggcctt gaccatgaca tggtcgccac gatggaggtt gtcggggctg
     1261 cacgggtccg cgggatgcgc ggcgtcgcat agccgctgcg gcggaaacac cttcggctgc
     1321 cggttgatcg gctcggtgta gagacagccg gccaggacca cgccggcgcc tgcgacgagc
     1381 cgcgcacgtg ccctgagccg cacgcggata gcctgccgca gcccctcgcc acccgcaacg
     1441 gagaacacgc gcaggacgcg gtccggtgca ggtcaccgga ccgcgcgcgc cggtcggatc
     1501 acgccacgtc gaagcggtcc aggttcatca ccttggtcca ggccgcgacg aagtcctgga
     1561 cgaacttgtt ctgcgcgtcc gagctggcat agacctcggc cagggcgcgg agcacggagt
     1621 tcgaaccgaa gaccagatcg acccgcgtgc cggtccactt gacctcaccg gtcttgcgat
     1681 cgcggccttc gaacacctcc tgcgcgtcgg agaccggctt ccacgccgtg cccatgtcga
     1741 gcaggttcac gaagaagtcg ttggtcagcg cctccggacg cttggtgaac acgccctgct
     1801 tggacggtcc gacgttggtg ccgaggaccc gcaggccgcc gatcaggacc gtcatctcgg
     1861 gcgcggtcag cgtcagcagc tgcgcccggt cgaccagcag ctcctcggac ggtaccgagt
     1921 agcgctcctt ctggtagttg cggaagccgt cgacgaccgg ctcgagcacg gcgaacgact
     1981 ccgcgtcggt ctgggcctgc gaggcgtcgg tccgccccgg cttgaacgga accgtcaccg
     2041 tctgaccggc gttccgcgcg gcctgctcga tgcctgcgca accgcccagg acgatcaggt
     2101 cggcgagcga gaccttcttc ccgccggact gcgcgccgtt gaactccttc tggaccttct
     2161 ccagcgcgcc gagcaccttc gccagctggg cgggctggtt cgccgcccag tccttctggg
     2221 gtgcgaggcg aatccgggca ccgttcgcgc cgccgcgctt gtcggagccg cggaaggtcg
     2281 acgccgacgc ccaggcggtc gacaccagct gcgagaccgt gaggcccgcg gcgaggatgt
     2341 tggccttgag cgtggcggcg tcccgctcgt cgatcaacgg gtggtcggcc gcggggaccg
     2401 ggtcctgcca gatcagctcc tccttcggaa cctccggccc gaggtagcgc gcgcgagggc
     2461 ccatgtcgcg gtgcgtgagc ttgaaccagg cccgggcgaa cgcgtccgcg aactgatccg
     2521 ggtgctgcat gaagcgccgg gagatcttct cgtaggcggg gtcgagccgc agggacaggt
     2581 cggtggtgag catggtgggc gcgtgacgct tcgccgggtc gtgcgcatcg ggcacggtgc
     2641 cggcgccgcc gccgttcttc ggccgccact ggttcgcgcc ggccgggctc ttgaacagct
     2701 cccactcgta gccgaacagg ttctcgaaga agttgttgct ccacttggtc ggcgtggtgg
     2761 tccaggtgcc ttccaggccg ctggtgatcg tgtgcccgcc gtggcccttg cccaggctgt
     2821 tcctccagcc gaagccctgc tcgtcgatgg cggctccttc gggctcgcgc gcgatgtgct
     2881 tggcctcctc gccggcgccg tgggtcttgc cgaacgtgtg accgcccgcg atcagcgcga
     2941 cggtctcctc gtcgttcatc gccatgcggc ggaaggtctc gcggatgtct cgcgccgccg
     3001 cgatcggatc cggattgccg ttcgggccct gcgggttgac gtagatgagg cccatctgga
     3061 cggcgcccag cggaccctcg agctcgcggt ctccggagta gcgcttgtcc gcgagccact
     3121 gggtctcgga gccccagtag acgtcgccct ggggctccca gatgtccgcg cgtccgccgc
     3181 cgaacccgaa ggtcttgaag cccatcgact ccagcgcgac gttaccggtg aggatgaaga
     3241 ggtcggccca cgagatcttg cggccgtacc tctgcttgat cggccagagc agccggcgcg
     3301 ccttgtcgag gttggcgtta tccggccagc tgttctcggg cgcgaaccgc tgggtgccgc
     3361 tgccggcacc gcctcgacca tcgccgatgc ggtacgtgcc ggcggcgtgc catgccatgc
     3421 ggatgaacag cggcccgtag tgaccgaagt ccgccggcca ccagtcctgc gagtcggtca
     3481 tcagcgccag gaggtctttc ttcagcgccg cgaagtccag gctcttgaac tccttggcgt
     3541 agttgaagtc ctcgcccatg ggattcgaca gcgaggatcg ctggtgcagg gtctcgagcc
     3601 gcaactgatt cggccaccag tcgcggttgg acgtgccgct ccccgcctgg tgcttgaaag
     3661 gacattttgc ttcggttgac attgttctct ccgtggatcc cgtcgcgggg gagagtatgc
     3721 ccgaagcggt cggggttgaa gtgggttggt cggatggttt cgataggcaa gtcttatcag
     3781 gcgaacctcg cagccccccg ccagcgcgag ccggcgaggt cgctcactcg gcgagcaccg
     3841 cgggtcttga cgaagccctg ggcgtcgagc acgacgcagc gctccagcag ctcggtgttc
     3901 ggcgaggcgc cggtcacatg accacgtggc cgatcgcgtg gccggcgcgc tcgccggtcc
     3961 gcctgtccag ccaggtgacg cgctcgagcg ccgcgtcgcc gccgagctcg acgatctcgg
     4021 tctcgacgag cagctggagg ttcggcgtct ccgcgatccg gcggttgcgc gacatctgct
     4081 gaacaggccg ctcgctcgta ccatgacgta caccttcacc ttgcgcgcgg accgggaagc
     4141 cgaggtggtt ctcgatcttg gagctcgtgc cggcctggcc gccgggcgcg atcgcctcga
     4201 ggacgaccga cagggccggc gcgccgcacg agttctcacc gtcggcggcg ctcacgccga
     4261 cgaacgcggg cctcggcatg cctccgagcg acccactgtc gcagcgtgcg ctctcgcctc
     4321 gcgtgcgcca gcggctaccc tggctcgcca atggccgtgc tcgatcgcga ccgtgcgcag
     4381 atctcgctcc tgcacctcgc ctggccgatg ttcgtcgaga acgtcctgcg agcatcgctg
     4441 atgagcgtgg atacggcgat gctcagccgg tactcggaga acgcggtcgc ggcgatgagc
     4501 ctggtcaacc agttcgcgtt cttcatccag ctgctctaca tgacggtgtc gaccggcgcg
     4561 tcgatcctga tcacgcagca gctcggcgcg gggcggccgc gcgaggcccg cctgatcgcc
     4621 gtcggcagcc tgaccctggt ggtcgcgttc tcgctcgtgg tgtcgtcggt gttcaccctc
     4681 gtcaccggcc cgctgatcga tctgtacggc ctcgaccccg aggtcgcgcg ctatgcgcgc
     4741 cagttcctcg tgctctacgg cgggctgtcg ttcttcatgg cggtcaacat cgcgcaggcc
     4801 agcatcctgc gtgcgtgggg tcacacccgc gacccgatgc tggtcaacgt gctgagcctc
     4861 gtgctcaccg tgctcggcaa cgccctgtgc ctgttcgggc cgttcggctt cccggtgctc
     4921 ggggtgaccg gcgtcgccgc gtcgacggtg ctcagccagg tggtcgcctg tgcgctgtgc
     4981 ttcgtcgcga tgcgccggcg gcgcgagctc gagctgccgc tcggcgaggc cgcgcggatc
     5041 cccggccggc tgtacagcgc gatcctcgcg atcggcatcc ccaccgccgg cgagaacctg
     5101 gcctacaacc tgtcgcagat cgcgatcatg gcgatgctgt cggcgctcgg cgccgtgccg
     5161 ctcgccacct acggcatcgt gatggcggtg ctgcgctacg tgttcatggc gggcgtgtcg
     5221 atcggctcgg ccgcgcagct caaggtcggc tacctggtcg gctcggggcg ccacgccgag
     5281 gccaagcagc gcgtgtatcg ctacttcgcg ctggcgtcgg tgtgcacgat ctcgtcggtg
     5341 ctgctgctcg agctgttcca cggctccgtc ctgggcctgt tctcgaccga tcgcgacatc
     5401 ctcgcgctgg cctcgacggt gctgctggtc gcggtcttcc acgagcccgg ccgcaacttc
     5461 aacaccgtgt tcatcccggc gctcaagggc gcgggcgacg tccggtttcc ggtcaccatg
     5521 ggcatcctct cgatgtgggg catcggcgtc gccggcgcgt ggctgctcgg catccgcctg
     5581 cactggggcc tccccgggat ctggatcgcg atggcggcag acgagtggct gcgcggcctg
     5641 gtgatgctgc gccggtggcg gtccggtgtc tggcaggcca gcggccggct cgaggcggcc
     5701 ggcgccgacg tcgccgaggt cgcggcggtg gagatcgagg aagggatctg atccgcgcag
     5761 aggtcgatca cagcgtcgag gcttctgccg gcgaccgtcg cgcggccatg ctagcgtgtc
     5821 gcgccatgcc cgcgccgacc gagccgcccg tccccgccgg cggcgatgga gcgggagagg
     5881 cgcccggaga ggtgcccgga gaggcgatcg acgaggcgcg gcgcacctcg ttcgaccaca
     5941 aggcggagct ctacgatgcg ccttgctcga cacgtactcc gatcacgcga cgctcgccga
     6001 ggaccggcgg cgcgcgctct acgccgacat cgccggcgtg atcgaccgtc acggcggccg
     6061 catcgagcta ccctatgtca cgctcgcgtt cctggcgttc gcgcgcgcgg ggtcgtg
//
LOCUS       DASVOR010001406         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393739,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001406 DASVOR010000000
VERSION     DASVOR010001406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393739"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..556
                     /locus_tag="VF516_26740"
     CDS             164..556
                     /locus_tag="VF516_26740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8111367.1"
                     /translation="MNTQEKNMLIAYDVALDLVRALRPVVAQLRQYSPDAADQVERAA
                     SSIVLNLAEGGRRHGRDPRRFWDMAHGSAGEIRGALDLADAWGWQVDSAQARVLLDRE
                     LRLLWGLTRRSSANHASVPASGARPRSR"
     gene            complement(565..1242)
                     /locus_tag="VF516_26745"
     CDS             complement(565..1242)
                     /locus_tag="VF516_26745"
                     /inference="COORDINATES: protein motif:HMM:NF025468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HEX8111368.1"
                     /translation="MKRGLVLVEGQTEERFVNECLAPYLLARGLVLERPTIVATKRVA
                     GGPNFKGGISSYGQVQRDLQRLLHDGHASVVTTVLDYYALPKDFPGMDSRPAGSPRSR
                     VEHVEAAWAVAVDDRRFVPHLALHEFEAWVYADPSRLEPFMFDDDPGVIEAIARIAAG
                     HSTPEHIDDGPMTAPSKRLREVFAAYQKPLHGPLAVSAIGLDRIRAVCPHFHQWLGRL
                     EAPTGVD"
     gene            complement(1248..2408)
                     /locus_tag="VF516_26750"
     CDS             complement(1248..2408)
                     /locus_tag="VF516_26750"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8111369.1"
                     /translation="MPAPDSVEAEGQKSPALDSIEVEGYKSIRSAEIRLGPINVLIGA
                     NGSGKSNLVGLFGLLADLADSRLQLHVARQGGANAIFHFGLKRTSQLRIALRFGLHGY
                     EAVFVPRAGDAIVFEDEATLAWRPASASSNTTPLGPPDRLRLGAGHQETELPWHPSSM
                     ADTFEDSKRVLDAMTSWRPFHFHDTGRSAPTKQKHKIDDNRALRAEGDNLAPFLFALR
                     TIAPGPYRRIVAAVRAIAPFFDDFVLEPDVINPGVIQLAWRHTMDDALFTADSLSDGT
                     LRFICLATLLLQPALPSLIVIDEPELGLHPFAIAQLAALVRAAATKAQVILATQSVTL
                     LDQFDASEVIVVDRSNGESTFTRHEESALAEWKQDYSLGELWLKNVLGGRPR"
     gene            complement(2423..>2942)
                     /locus_tag="VF516_26755"
     CDS             complement(2423..>2942)
                     /locus_tag="VF516_26755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111370.1"
                     /translation="DLAAPDRRAHALAAAGAVASRYLAVGTPRSFGIVINDVAEVDAA
                     ALSLAAHRTWFAPRDIRCASTASGAAELATATGGHTVSLDEALACDIVNVYSPCIRIT
                     PVQLRRGTHVNALTAAVFAPELLVLATVVPEVGLLALAAGLVDGRQLDELTIFVISDG
                     PSLATFDRGQVP"
BASE COUNT          489 a          928 c         1044 g          481 t
ORIGIN      
        1 aacgccaccg cgcccgggtg gcgccaccac cgccgcggag accggcctcc ggaagaccac
       61 atgcgctcgc gtgggcgttc gtcacgggtc ctggcgcgga tcggtcgcga cgggcggagc
      121 ccagccgcgg aggtcgtcgg caaagggcgt tcccctccag aagatgaaca cccaggagaa
      181 gaacatgttg attgcttatg acgttgcgct cgatctggtt cgcgcactgc gccccgtggt
      241 cgcgcagctc cggcagtaca gccccgacgc cgccgatcag gtcgagcggg cggcgagcag
      301 catcgtgctc aacctcgccg aaggcggtcg ccgccatggc cgtgatccca ggcggttctg
      361 ggacatggcg cacggaagtg ccggcgagat ccgcggtgca ctcgatctgg cagatgcgtg
      421 gggctggcag gtcgacagcg cgcaggcgcg tgtgctgctc gatcgcgagc tccgcctcct
      481 gtggggactc acccgccgca gcagcgccaa ccacgcctcg gtcccagcgt caggagccag
      541 accgcgctcg cgctgacccg actgtcaatc aacgcctgtc ggggcttcca ggcgcccgag
      601 ccattgatgg aagtgcggac acaccgcgcg aatgcggtcg aggccgatcg cggacacggc
      661 gagcgggccg tgcaacggct tctggtaggc cgcgaacacc tcacgcagtc gcttcgacgg
      721 tgcagtcatc ggaccgtcat cgatgtgttc gggggtcgaa tgccctgccg cgatcctggc
      781 gatcgcttcg atgaccccgg ggtcgtcgtc gaacatgaag ggttccagcc tcgacggatc
      841 ggcatagacc caggcctcga attcgtgcag tgcgaggtga ggaacgaagc gccggtcatc
      901 gacagcgaca gcccaagcgg cctctacatg ctctacgcgg ctgcgagggc ttcctgcagg
      961 cctgctgtcc atgccaggga agtccttcgg cagcgcgtag tagtcgagca ccgtggtgac
     1021 gaccgacgca tggccgtcgt gaagcaggcg ctggaggtcg cgctggacct gaccatagct
     1081 gctgatgccg cccttgaagt tggggcctcc ggcgacgcgc ttcgtcgcga cgatcgtggg
     1141 tcgctccagg accagccccc tggcgaggag gtagggagcg aggcactcgt tgacgaaccg
     1201 ctcctcggtc tgcccctcga ccaacacgag cccccgcttc atcgcgctca ccgcggccga
     1261 ccgcccagca cgttcttgag ccacagctcg ccgagcgagt agtcctgttt ccactcggcg
     1321 agcgccgact cttcgtggcg agtgaaggtg gattcgccgt tgctccggtc caccacgatc
     1381 acctcgctgg catcgaactg atcgagcagt gtcacggact gggtcgccag gatcacctgg
     1441 gccttcgtgg ctgccgcgcg gaccagcgcg gcgagctgcg cgatcgcgaa cggatgcagc
     1501 ccgagctctg gctcgtcgat cacgatgagc gatggtaacg ccggttgcag cagcagagtc
     1561 gccaggcaga tgaagcgcag tgtgccgtcg gacaatgagt cagctgtgaa cagcgcgtcg
     1621 tccatggtgt ggcgccacgc aagctggatc acgccagggt tgatcacgtc gggctcgagg
     1681 acgaagtcgt cgaagaacgg cgcgatcgcg cgtacggctg ccacgatgcg gcgatagggg
     1741 cccggtgcga tggtgcgcag cgcgaacagg aacggcgcga ggttgtcgcc ctcggccctc
     1801 agcgctcgat tgtcgtcgat cttgtgcttc tgcttcgtag gtgccgatcg ccctgtgtcg
     1861 tgaaaatgga acggccgcca gctggtcatt gcgtcgagaa cgcgcttcga gtcctcgaac
     1921 gtgtcggcca tgctactcgg atgccagggt agctctgtct cttggtgccc ggctcccaac
     1981 cgcaggcgat ctggggggcc tagcggtgtg gtattgcttg atgctgacgc tggcctccac
     2041 gccagcgttg cctcgtcttc gaatacgatc gcgtccccgg ctctcggaac gaacaccgct
     2101 tcatacccgt gtaaaccaaa gcgcagtgcg attcgcaact gggaagtcct cttgagcccg
     2161 aaatggaaga tcgcgttggc tccgccttgg cgggcgacgt gaagctgaag tcggctatca
     2221 gcgaggtcgg cgagcaggcc gaacagacca acgaggttgg actttcccga accgttggca
     2281 ccgatcaaca cgttgatcgg gcctagacgg atctctgccg agcggatcga cttgtagccc
     2341 tccacttcga tggagtcgag ggcaggtgat ttctggccct ccgcttcgac ggagtcgggg
     2401 gcaggcatct cggttgaggt tgtcacggaa cctggcccct gtcgaacgtt gccaggctcg
     2461 ggccgtcgct gatgacgaag atcgtgagct cgtcgagctg gcggccgtcg accaggccgg
     2521 cggcgagggc gaggaggccg acttcaggca ccacggtcgc gagcacgagc agctcgggag
     2581 cgaagacggc ggcggtgagg gcgttgacgt gggtgccgcg gcgaagctgt acgggggtga
     2641 tacggatgca cgggctgtag acgttgacga tgtcgcacgc gagggcctcg tcgagcgaga
     2701 cggtgtggcc gccggtggcg gtggcgagct cggccgcgcc cgaggccgtc gatgcgcagc
     2761 ggatatcgcg cggggcgaac caggtgcggt gggcggcgag cgacagcgcg gcggcgtcga
     2821 cctcggcgac gtcattgatc acgatgccga agctgcgcgg cgtgccgacg gcgaggtagc
     2881 gcgaggcgac ggcgcccgca gctgcgaggg cgtgggcgcg gcgatccggc gcggcgaggt
     2941 cg
//
LOCUS       DASVOR010001407         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_102812,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001407 DASVOR010000000
VERSION     DASVOR010001407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102812"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VF516_26760"
     CDS             <1..1015
                     /locus_tag="VF516_26760"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8111371.1"
                     /translation="LLDPKVPLTEDGARELLFVHGFSTRQGASSLSGRGVGLDAVRRR
                     LAAIGGRVSVTSTPGAGTRFAIHIPSTVFRERVLIADVGGALCALPAREVEAVVPINP
                     SMMEQVGASPLLRYRGQGIPFLPLPLFRADADSTRAAIVLHGGHRWAISLPEVVGDVE
                     LLRSPVDAAVSALGPFAASGTLDDGRLVLVVSLDALLSNPRQVAHFSRAPLRDRGRRR
                     VLVVDDSPIVRDLLSELLSSVGLEVRTAGDGAVALQSLADHPVDLIVCDVEMPVMDGF
                     EFLRRLRDRADRVPVVMVTTRGSVSDRATAASLGADALIVKSEFENAQLLDTVRRFVE
                     ISA"
     gene            1012..>1563
                     /locus_tag="VF516_26765"
     CDS             1012..>1563
                     /locus_tag="VF516_26765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8111372.1"
                     /translation="MKRAVVVDDSAIWGTQLREWLEADGDVEVVGVAADGESAIDLIT
                     RLRPDVATIDLRLPGMSGLEVVTLLMARAPLPLLVLTGDPTGDDPELAFEAIRRGALD
                     LLLKPSMDDEEAIRALRAHVRWLAGVPVVRHLDRGDGHRPGSEPAIRVPEFLRTSADA
                     SRPHEIRLNDVRPNDVRPAAVPAS"
BASE COUNT          169 a          579 c          583 g          232 t
ORIGIN      
        1 cctgctggat cccaaggtgc cgctcaccga ggacggcgcg cgcgagctgt tgttcgtgca
       61 cgggttctcg acccgccagg gtgcgagcag cctctcgggg cgcggggtcg ggctcgacgc
      121 cgtgcgccgc cggctggcgg cgatcggcgg ccgggtctcg gtgaccagca cgccgggcgc
      181 cggcacgcgg ttcgcgatcc acatcccgtc gacggtgttc cgcgagcgcg tgctgatcgc
      241 cgacgtcggc ggggcgctgt gcgcgctgcc ggcgcgcgag gtcgaggcgg tggtgccgat
      301 caacccgtcg atgatggagc aggtcggcgc gtcgccgctg ttgcgctacc gcggccaggg
      361 catcccgttc ctgccgctgc cgctgttccg cgccgacgcc gacagcacgc gcgccgcgat
      421 cgtgctgcac ggcggccacc gctgggcgat ctcgctgccc gaggtggtcg gcgacgtcga
      481 gctgctgcgc tccccggtcg acgccgcggt gtccgcgctc ggcccgttcg ccgcgagcgg
      541 cacgctcgac gacggccgcc tggtcctggt ggtcagcctc gacgcgctgc tgtcgaaccc
      601 gcgccaggtc gcccacttct cgcgcgcccc gctccgcgat cgcggccggc gccgggtgct
      661 ggtggtcgac gactcgccga tcgtgcgcga cctgctcagc gagctcttgt cgagcgtcgg
      721 gctcgaggtc cgcaccgcgg gcgacggcgc cgtcgcgctg cagagcctcg ccgaccaccc
      781 ggtcgacctg atcgtgtgcg acgtcgagat gccggtgatg gacggcttcg agttcctgcg
      841 ccggctgcgc gaccgcgccg accgcgtccc ggtggtgatg gtgaccaccc gcggctcggt
      901 gtccgaccgc gcgaccgccg cgtcgctcgg cgccgacgcg ctgatcgtca agtcggagtt
      961 cgagaacgcc cagctgctcg acaccgtgcg gcggttcgtg gagatctctg cgtgaagcgt
     1021 gccgtcgtgg tcgatgattc ggcgatctgg ggcacccagc tccgcgagtg gctggaggcc
     1081 gacggcgacg tcgaggtcgt cggggtcgcg gccgacggcg agtccgcgat cgacctgatc
     1141 acccggctgc gccccgacgt cgcgacgatc gacctgcggc tgcccgggat gtcggggctc
     1201 gaggtcgtga ccctgctgat ggcgcgcgcg ccgctgccgc tgctggtcct gaccggcgat
     1261 ccgaccggcg acgatcccga gctcgcgttc gaggcgatcc ggcgcggtgc gctcgacctc
     1321 ctgctcaagc cgagcatgga cgacgaggag gcgatccgtg cgctgcgcgc ccacgtgcgc
     1381 tggctcgccg gcgtgccggt ggtccgccac ctcgaccgcg gcgacggcca ccgcccgggc
     1441 tccgagccgg cgatccgggt tcccgagttc ctgcgcacca gcgccgacgc gagccggccg
     1501 cacgagatcc ggttgaacga cgtccgtccg aacgacgtgc gcccggcagc ggttccagcc
     1561 tcg
//
LOCUS       DASVOR010001408         4880 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_276628,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001408 DASVOR010000000
VERSION     DASVOR010001408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4880
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276628"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VF516_26770"
     CDS             complement(<1..436)
                     /locus_tag="VF516_26770"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8111373.1"
                     /translation="MFTIHIQEKGGEQRRMVFNKPEVTIGRVQGNDIVLPKGNVSKRH
                     ARIVLKDGKFIIVDLKSTNGTYVNGRKITSPLVVKDSDKIYIGDFIVGVDEAASSEGD
                     GASEPTAGPPSERPVATPDARPPRPTEAQPMPPSALPMTGGVD"
     gene            755..1342
                     /locus_tag="VF516_26775"
     CDS             755..1342
                     /locus_tag="VF516_26775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111374.1"
                     /translation="MRGIRSLSLLGVAAAIALAAAPAGAAPKKKYHFELTAVSAKPEV
                     KPDVAKLATPRIDAQVRKVFAGHPQLVAKLDGAPDPRSSADAYRRYLTQHGLSGAYLV
                     TVEITEASEALEPLDDKTSQRLVIRIGIHMLGETIPGRTMGFTGDGRATIKQEVGIKL
                     RDRDREYTWDQAAELAVDDAMKTVFQQLAAPPPKR"
     gene            complement(1345..2475)
                     /gene="lepB"
                     /locus_tag="VF516_26780"
     CDS             complement(1345..2475)
                     /gene="lepB"
                     /locus_tag="VF516_26780"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8111375.1"
                     /translation="MRAASLDHRVRKEAELLVREARAALALKRNLRGKAGDLAAAVRD
                     IDAAMAAKDLHQVRRGLPALDALVDEVIKRPDKSTTRDYIESIVAAILIALALRAVVL
                     EAFKIPSSSMYPTLEINDHIFVNKFIYGIRIPFTTTKLFEWRKPRRGEVIVFIQPCEP
                     DRDYIKRVVATEGETVEVRCNVVHVDGKPVESQLVKGEGCSYEDQDETTLRWSSRDCS
                     EYVERVDGHEYHTYHDPGRPLRDERLAREGGLLSGDNRDFPIVDHARIPPSCPMQADG
                     QPAGTHNQKPGEIVVTKPNAPVCELQMHYVVPPGHVFVMGDNRNNSNDSRYWGSVPIE
                     NIKGKALFIWLSYGKVEGTNVFSQLVGFARSLRLDRIGNFVQ"
     gene            complement(2475..4277)
                     /gene="lepA"
                     /locus_tag="VF516_26785"
     CDS             complement(2475..4277)
                     /gene="lepA"
                     /locus_tag="VF516_26785"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEX8111376.1"
                     /translation="MSFPAERIRNFSIIAHIDHGKTTLADRILEACGAVTAREAQAQM
                     LDNMELERERGITIKAQSVQLHYKADDGQVYQFHLIDTPGHVDFHYEVSRSLAACEGA
                     LLVVDAAQGVEAQTLANVYVALDNHLEIVPVLNKIDLPVARPDFVKQQIEDTIGLDAS
                     DALLVSAKTGQGVHEMLEAVIERIPPPEADRDAPLSALIFDSWYDSFRGVVVLVRVMS
                     GVIRKGQKLRFWATDQVYECTMTAVRRPGPLEVDEIAAGQVGIVAASIKDIAHARVGD
                     TVTDADRPADEPLPGFKTVKPMVFAGLFPVDAADYQDLKDALGKLVLNDASVTYEPET
                     SAALGFGFRTGFLGLLHMEIIQERLEREFNLNLITTAPSVRYRITVPGQGVLEVDNPA
                     KVPDQGKVDQIEEPIITATVHLPQEYVGPIMQLCQERRGVQKGLHYDAGGRVRVVYEM
                     PLAEIVFGFYDRLKTLSRGYASLDYEPAGYRVADLIRLDVLVNGERVDALSIISHRDT
                     AYHRGVELCTKMKDVVPRQMFDVAIQAAIGSRVIARTTVKAVRKDVTAKCYGGDITRK
                     KKLLEKQKEGKKRMKSVGSVELPQEAFLTVLKVD"
     gene            complement(4297..4482)
                     /locus_tag="VF516_26790"
     CDS             complement(4297..4482)
                     /locus_tag="VF516_26790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEX8111377.1"
                     /translation="MSRKKISTTVYITPEQDSQLKALHQRTKVPVAEYIRQGIDLVLE
                     KYRDQLPGQVDLRLGSR"
     gene            4572..>4880
                     /locus_tag="VF516_26795"
     CDS             4572..>4880
                     /locus_tag="VF516_26795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111378.1"
                     /translation="MAKLLELVGSSPVLGSVLGVLVLGACHGKQEDTTPKTDTADSGV
                     PKVDPTLCDTNGKNVVTYDLNHDGKPDVWRLYKTEDDGGTKVEWLTCKQVDFDHDGRK
                     D"
BASE COUNT          749 a         1663 c         1643 g          825 t
ORIGIN      
        1 cgtcgactcc gccggtcatc ggcagcgcgg acggcggcat cggctgggcc tcggtcggtc
       61 gcggcgggcg cgcatccgga gtcgcgaccg ggcgctccga cggcggcccg gcggtcggct
      121 ccgacgcgcc atcgccctcc gacgacgcgg cctcgtcgac cccgacgatg aagtcgccaa
      181 tgtagatctt gtccgaatcc ttgaccacca gcgggctggt gatcttgcgg ccgttgacgt
      241 acgtcccgtt ggtgctcttg aggtcgacga tgatgaactt gccgtccttg agcacgatgc
      301 gcgcgtggcg cttggacacg ttgcccttgg gcaagacgat gtcgttgcct tgcacgcgac
      361 cgatggtgac ctcgggcttg ttgaacacca tgcggcgctg ctcgccgcct ttttcttgta
      421 tgtggatggt gaacattggc tcctgaaggt ctcccggtag gcgcccctgc ggaacggtac
      481 taagccgcgg taaccacggt caagttggcg actgatatgt ctgccctggt gcgaccgggg
      541 cggtgggttc ggacgccccg ccggccggcc cgagatgcaa gaccgaccgc acatcgtccc
      601 accacaggga ccagaccccg gcgagcagca gccccaggac ggccgcgacc agcagcgcat
      661 cgtgccatcg cggccggacc atgaatgcac gctagcatgt cgccgtctca caggaaatcg
      721 cccacccccc aggaggtctt cgatgccgtc tccgatgcgt ggcatccgtt cgctcagctt
      781 gctcggtgtc gcggccgcga tcgccctcgc cgccgcgccc gccggcgccg ccccgaaaaa
      841 gaaatatcat ttcgagctca cggcggtatc cgcgaagccc gaggtcaagc ccgacgtcgc
      901 caagctggcg acgccgcgca tcgacgccca ggtccgcaag gtgttcgccg gtcatcccca
      961 gctggtcgcc aagctcgacg gcgcccccga cccgagatcg agcgccgacg cctaccggcg
     1021 ctacctgacc cagcacggcc tgtccggcgc gtacctggtg accgtcgaga tcaccgaggc
     1081 ctcggaggcg ctggagccgc tcgacgacaa gacctcgcag cgcctggtga tccggatcgg
     1141 catccacatg ctgggcgaga ccatcccggg ccgcacgatg gggttcaccg gcgacggccg
     1201 ggcgacgatc aagcaggagg tcgggatcaa gctgcgcgat cgcgatcgcg agtacacctg
     1261 ggaccaggcc gccgagctcg cggtcgacga cgcgatgaag accgtgttcc agcagctcgc
     1321 cgcgcccccg cccaagcggt agggctactg gacgaagttg ccgatccggt ccagccgcag
     1381 gctgcgggca aagcccacca gctggctgaa cacgttcgtg ccctcgacct tgccgtacga
     1441 cagccagatg aacagcgcct tgcccttgat gttctcgatc ggcaccgagc cccagtagcg
     1501 cgagtcgttc gagttgttgc ggttgtcgcc catgacgaac acgtggccgg gcggcacgac
     1561 gtagtgcatc tggagctcgc acaccggcgc gttcggcttg gtcacgacga tctcgccggg
     1621 tttctggttg tgggtgccgg ccggctggcc gtcggcctgc atcgggcagc tcggcgggat
     1681 gcgcgcgtga tcgacgatcg ggaagtcgcg gttgtcgccg gacagaaggc cgccctcgcg
     1741 ggcgagccgc tcgtcgcgca gcggccggcc cgggtcgtgg taggtgtggt actcgtggcc
     1801 gtcgacccgc tcgacgtact cgctgcagtc gcgcgacgac cacctgagcg tggtctcgtc
     1861 ctggtcctcg tacgagcagc cctcgccctt gaccagctgg ctctcgaccg gcttgccgtc
     1921 gacgtggacc acgttgcagc ggacctcgac ggtctcgccc tcggtggcga ccacgcgctt
     1981 gatgtaatcg cggtccggct cgcacggctg gatgaacacg atcacctcgc cgcgcctggg
     2041 cttgcgccac tcgaacagct tggtggtcgt gaacgggatc cggatcccgt agatgaactt
     2101 gttgacgaag atgtggtcgt tgatctcgag cgtcgggtac atcgagctcg acgggatctt
     2161 gaacgcctcg agcaccaccg cgcgcagcgc gagcgcgatc aggatcgcgg cgacgatcga
     2221 ctcgatgtag tcgcgcgtgg tcgacttgtc ggggcgcttg atcacctcgt cgaccagcgc
     2281 gtcgagcgcc ggcaggccgc ggcgcacctg gtggaggtcc ttggcggcca tcgcggcgtc
     2341 gatgtcccgg accgccgcgg cgaggtcgcc ggccttgccg cgcaggttgc gcttgagcgc
     2401 gagcgccgcc cgggcctcgc gcacgagcag ctcggcctcc ttgcggaccc ggtggtcgag
     2461 gctggccgcg cgcatcagtc gaccttcagc acggtcaaga aggcttcctg cgggagctcg
     2521 acggagccga ccgacttcat acgcttcttg ccttccttct gcttctccag cagcttcttc
     2581 ttgcgcgtga tatcgccacc gtaacacttc gcggtgacgt ccttgcggac cgccttgacc
     2641 gtcgtgcgcg cgatcacgcg gctgccgatc gcggcctgga tcgcgacgtc gaacatctgg
     2701 cgcggcacga cgtccttcat cttcgtgcac agctcgacgc cgcggtggta cgcggtgtcg
     2761 cggtggctga tgatcgacag cgcgtcgacc cgctcaccgt tgaccagcac gtcgagccgg
     2821 atcaggtccg ccacgcggta gccggccggc tcgtaatcca gggaggcgta gccgcgcgac
     2881 aaggtcttga gccgatcgta gaacccgaac acgatctccg ccagcggcat ctcgtagacc
     2941 acgcggaccc ggccgccggc gtcgtagtgc agccccttct gcacgccgcg gcgctcctgg
     3001 cacagctgca tgatcggccc gacgtactcc tgcggcaggt gcacggtggc ggtgatgatc
     3061 ggctcctcga tctggtcgac cttgccctga tcggggacct tggcggggtt gtcgacctcg
     3121 agcacgccct ggcccggcac ggtgatccgg tagcgcacgc tgggcgcggt ggtgatcagg
     3181 ttgaggttga actcgcgctc gagccgctcc tggatgatct ccatgtggag cagaccgagg
     3241 aacccggtgc ggaacccgaa gccgagcgcc gccgaggtct cgggctcgta ggtcaccgac
     3301 gcgtcgttga gcacgagctt gccgagcgcg tccttgaggt cctggtagtc ggcggcgtcg
     3361 accgggaaca acccggcgaa caccatcggc ttgaccgtct tgaacccggg cagcggctcg
     3421 tcggccggcc ggtcggcgtc ggtcaccgtg tcgccgaccc gggcgtgggc gatgtccttg
     3481 atcgacgccg cgacgatccc gacctggccg gccgcgatct cgtcgacctc gagcggcccc
     3541 ggccgccgca cggcggtcat cgtgcactcg tagacctggt cggtggccca gaaccggagc
     3601 ttctggccct tccggatcac gcccgacatc acgcggacca gcacgaccac gccgcggaac
     3661 gagtcgtacc agctgtcgaa gatcagcgcc gacagcggcg cgtcgcggtc ggcctcgggc
     3721 ggcgggatgc gctcgatcac cgcctcgagc atctcgtgca cgccctgccc ggtcttggcc
     3781 gacaccagca gcgcgtccga cgcgtcgagc ccgatcgtgt cctcgatctg ctgcttgacg
     3841 aagtcgggcc gcgcgaccgg caggtcgatc ttgttgagca ccggcacgat ctcgaggtgg
     3901 ttgtcgagcg cgacgtagac gttggccagg gtctgggcct cgacgccctg tgcggcgtcg
     3961 accaccagca gcgcgccctc gcacgcggcc agcgagcgcg acacctcgta gtggaagtcg
     4021 acgtggcccg gcgtgtcgat caggtggaac tggtagacct gcccgtcgtc ggccttgtag
     4081 tggagctgga ccgactgcgc cttgatcgtg atgccgcgct cgcgctcgag ctccatgttg
     4141 tcgagcatct gggcctgggc ctcgcgcgcg gtgaccgcac cgcacgcctc gaggatccga
     4201 tcggccagcg tggtcttgcc gtgatcgatg tgggcgatga tcgagaagtt acggatccgc
     4261 tccgcgggaa acgacatggg aaggcgccgg ccgtacttag cgtgatccga gccgcaggtc
     4321 gacctgcccc ggcagctgat cgcggtactt ctccagcacg aggtcgatac cctgccggat
     4381 gtactcggcg accgggacct tggtgcgctg gtgcagggct ttcagctggc tatcctgctc
     4441 cggcgtgatg tacaccgtcg tcgagatctt ctttcgagac accgccctgt gaccctacgt
     4501 gaccatatgc gcggggtcaa atggtcttgg gaggttgccg cgacccgggt ccaagactag
     4561 gctttctggc gatggcgaaa ctcttggaac tcgtcgggtc ttccccagtt ctgggctccg
     4621 ttctgggcgt cctggtcctg ggggcgtgtc acggcaagca ggaggacacg acgccgaaga
     4681 ccgacaccgc ggacagcggc gtcccgaagg tcgatcccac gctgtgcgac accaacggta
     4741 agaacgtcgt cacgtacgac ctcaaccacg acggcaagcc cgacgtgtgg cggctgtaca
     4801 agaccgagga cgacggcggc accaaggtcg agtggctgac ctgcaagcag gtcgacttcg
     4861 accacgacgg ccgcaaggac
//
LOCUS       DASVOR010001409         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_15256,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001409 DASVOR010000000
VERSION     DASVOR010001409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15256"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="VF516_26800"
     CDS             complement(<1..246)
                     /locus_tag="VF516_26800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111379.1"
                     /translation="MKLESIVARTFMSFAAIGLVFSTFGCGPQQQDADGDEEREVEEL
                     IVTAAGSCGVERWAVKTGTDSTANLVNLTPRDTTIASL"
     gene            1504..1875
                     /locus_tag="VF516_26805"
     CDS             1504..1875
                     /locus_tag="VF516_26805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111380.1"
                     /translation="MTAGIAYHGAMRCLVLATALFLSACAAESANGPGAQKPRTGDEI
                     VCKEEATTGSAIARRVCRGPEQRQNEESFKQSWMTGWNPHNPMRGDFTYPGMDLRHPP
                     PPPSTETTYVPPSPAPSPPSD"
     gene            1988..3271
                     /locus_tag="VF516_26810"
     CDS             1988..3271
                     /locus_tag="VF516_26810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111381.1"
                     /translation="MLDATQALTVLAEIVPGPPRALLEQRLAVIAADLDGNPIFRPCD
                     LPDTHFMRLVIVDDPRGEFPALLAWESNHDGRAAEYLAAVARTVPAITAVFECCAGYP
                     AEGTADVERWVAWMLARAPRAAAFYAGYRGVPRRAVVNARRVHDAVREVLDGDRAALR
                     NLSRSDLQIQIRERVAARHPELDLSRDGPEGNDELRWALGKLAAAVVGLALLPLVLVV
                     APIWYLLLRRCEDRDIATAYARPLFIDPAVRCAEDQVTQNQMTHVVDVKPGAFRLATL
                     WLVLTAIDVAARVWSVHGELGGITAIHFARWVILRDRRPVSARRHRLLFFSNYDFSWQ
                     SYLGEFIDRAAFGLTAVWSNTRGFPATRHLVLDGAHDEEAFKQWTREHQLPTQVWWSG
                     VPDSTVQNVRDDVEIRQQLARGLADHEIETWLGKL"
     gene            3268..>3528
                     /locus_tag="VF516_26815"
     CDS             3268..>3528
                     /locus_tag="VF516_26815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111382.1"
                     /translation="MTRPADARELAVPGADDLEDIQGLVYSAWTDHPYAGFLFARLGD
                     DARASRAWLDAVRRQVTPAARHRRRTHGRLQLALSASGLGALG"
BASE COUNT          542 a         1262 c         1166 g          558 t
ORIGIN      
        1 cagactggcg atcgtggtat ctcgcggcgt caggttgacg aggttcgcgg tcgagtccgt
       61 gcccgtcttg acagcccagc gctcgacgcc gcagctgccg gcggcggtga cgatgagctc
      121 ctcgacctcg cgctcctcat ccccatcagc gtcctgttgt tgcggaccac acccgaacgt
      181 cgaaaagacc agtccgatgg cggcgaatga catgaacgtg cgagcgacaa tggattcgag
      241 cttcacgatg cctccttgtt ccgtggaagt tcggcagtga cgtacccacg atccgtccgg
      301 cacctcgcga gggcaatgcg cgtatcgcta aaaaatctgc gtggaggtcg aatatgtcga
      361 gatcacacga tcgccacggg attgttacct atgaaactat agggtcacat caagactgat
      421 gtacatcgcc attgttcatc gaatgtccgc ttcatgccta caccatgcca gaccaaccgc
      481 atacgccaca gtggtcgatc gtcatctacg cgttggtcaa gatcggtaag cccgagccag
      541 acttcaggtt tcgcatgacg agaccgcgcg acacgacgtg tattgcatgc actgacaagt
      601 ctgcgcttgc cagccatact cgaacatggt gcgccggcca tcgatccgga aaacttcaga
      661 ggccggcggg cgcagcgcgg tcggcggcag cgctcgcgct ggccctcgtt cgcggtggtc
      721 cgcagtcgag gcgccggcgc atcgaccttg cgccaccgag ctgattgatg cccaccgctc
      781 ggtcagtaaa aggcgcgcgc ctcgtcgctg gagatgcgaa gcaggacgag ataatgcgac
      841 caggcgagcg cgctcgggaa gtgcaccgcg atcggcgccg agggcggcgc gatcccaggt
      901 ggtttggccc gagcagaaga tattctcgac ggtgtcgaga aattctccgc gggcgcattc
      961 ccgatcgcgg aaccaccggg aaatgctaga taaaactgac gcatccggaa gaggttcggc
     1021 tggctgaagc cctttccgta cgtccgctgg aggcgcttcg cgagaccttt gacgacctcg
     1081 ttcccatagc gagcccgggg tgaccctcct gagtgaccgc gacgatctca cgaccgatca
     1141 gccagtaggc ctggaccatc gccgtattga ctgtacgagc gacctgcccc ctcgcgtgtt
     1201 ccaggatctg cgcgattcgc tcgtagagcc cgtcatcggt catgcggaga gttcctcctc
     1261 catgtcgttc gccgcatcgt cggcccatgg cgcctcgagg tcgaggttcc tgagcgcgtt
     1321 gtcgtcggaa tttccgcctg cagagcctca gcccgtttgc ggagccgacg ctgctccgcg
     1381 gacgaccagc gcctcgagcg ccgcgcgcag aaggcccgcg agttcgccgc aacccccggc
     1441 tacccgcctg ccgaggcgcg tgccacagct tgcctggcga gccaccctgc atttgcagga
     1501 tccgtgaccg ccgggatcgc gtaccatggc gccatgcgct gcctcgtgct tgcaaccgcc
     1561 ctgttcctct ccgcctgcgc ggccgaatcc gcgaacgggc cgggcgcgca gaagccgcgg
     1621 accggcgacg agatcgtctg caaggaggag gcgacgaccg gcagcgccat cgcgcgccgg
     1681 gtctgccgcg ggccggagca gcgtcagaac gaagagtcct tcaagcagtc gtggatgacc
     1741 ggctggaacc cccacaaccc gatgcgcggc gacttcacgt atcccggcat ggaccttcgc
     1801 cacccgccgc cgccgcccag cacggagacg acgtacgtgc cgccgtcgcc ggcaccgtcg
     1861 ccgccgtcgg actgacgcgc ggcgcgccgt gccgctgctg gccgccgatc tcggggagct
     1921 ctcccgccgg ccaaaatgat cgcctgggcc cccgtggcgc tcccgggtgg tcgatactgg
     1981 ccggcgcatg ctcgatgcca cgcaggcgct gacggtgctc gccgagatcg tgcccggccc
     2041 accgcgcgcc ctgctcgagc agcggctcgc cgtgatcgcg gccgatctcg acggtaaccc
     2101 gatcttccgg ccgtgcgatc tgcccgatac ccacttcatg cggctggtca tcgtcgacga
     2161 tccgcgcggg gagttcccgg cactgctggc ctgggagagc aaccacgacg ggcgcgccgc
     2221 cgagtacctg gccgccgtcg cgcgcaccgt cccggcgatc accgcggtgt tcgagtgctg
     2281 cgccggctac cccgcggagg gcaccgccga cgtcgagcgc tgggtggcgt ggatgctcgc
     2341 ccgcgccccc cgcgccgcgg cgttctatgc cggctaccgc ggcgtgccgc gtcgtgcggt
     2401 ggtcaacgcg cgccgcgtcc acgacgcggt ccgcgaggtg ctcgacggcg atcgcgctgc
     2461 gctgcgcaac ctgtcgcgca gcgatctcca gatccagatc cgcgagcggg tcgccgcgcg
     2521 ccaccccgag ctcgacctgt cgcgcgacgg ccccgagggc aatgacgagc tgcgctgggc
     2581 gctcggcaag ctcgcggcgg cggtggtcgg gctcgcgctg ctgccgctgg tgctcgtcgt
     2641 cgcgccgatc tggtacctgc tgctccgccg ctgcgaggac cgcgacatcg cgaccgcgta
     2701 cgcccggccg ctgttcatcg atcccgcggt gcgctgcgcc gaggatcagg tcacccagaa
     2761 ccagatgacc cacgtggtcg acgtcaagcc gggcgcgttc cggctcgcga cgctgtggct
     2821 ggtgctcacc gcgatcgacg tcgccgcgcg ggtgtggtcg gtgcacggcg agcttggcgg
     2881 catcaccgcg atccacttcg cgcgctgggt gatcctccgc gaccgccggc cggtcagcgc
     2941 gcgccgccac cgcctgctgt tcttctccaa ctacgacttc agctggcaga gctacctcgg
     3001 cgagttcatc gaccgcgcgg cgttcgggct gaccgcggtg tggtcgaata cacgggggtt
     3061 ccctgccacc cgccacctgg tgctcgacgg cgcgcacgac gaggaggcgt tcaagcagtg
     3121 gacccgcgag caccagctgc cgacccaggt gtggtggagc ggcgtgcccg actccacggt
     3181 gcagaacgtg cgcgacgacg tcgagatccg gcagcagctc gcgcgcggcc tcgccgacca
     3241 cgagatcgag acctggctgg ggaagctatg acccgacccg cagatgcccg cgagctcgcc
     3301 gtgcccggcg ccgacgacct ggaggacatc cagggcctgg tctacagcgc gtggaccgat
     3361 cacccctacg ccggctttct gttcgcacgg ctcggcgacg acgcgcgggc ctcgcgcgcc
     3421 tggctcgacg ccgtccggcg ccaggtcacc ccggccgcgc ggcaccgccg ccggacccac
     3481 ggccggctcc agctcgcgct gtcggccagc gggctcgggg cgctcggc
//
LOCUS       DASVOR010001410        11063 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_219110,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001410 DASVOR010000000
VERSION     DASVOR010001410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219110"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(326..1447)
                     /locus_tag="VF516_26820"
     CDS             complement(326..1447)
                     /locus_tag="VF516_26820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111383.1"
                     /translation="MAEEPRPGLIVGGRYELIEVAGRGGMADVWRGRVRGDSGFVRDV
                     AIKQMHANLTTQPPYVAMFVEEARVGAVLKSPNISEVHDFVHDRGHYYMVLEWIDGVD
                     LGSWIKWHVARNEPTRWELVAAVAVGVLRALAAAHERTGPDGAPLPIIHRDVSPHNVL
                     LTTRGVVKLIDFGLAFAPDRAQETTDPGIVKGKMSYLAPEIVTGGRPSPASDQFACGS
                     VLWEALVGRKLFDGETDYEAYCRLRDCLVQPLRPLRPDVPPAFAQIIHRALSAEIDDR
                     FPSTREMARQIGTALKKVQLRRDLHSVLSRSVSEARAELGLGGIIAELSDVTPLAELL
                     PDEPPRLEPAPRGEGAAAQAFERLRSLRHYLPFFGKKRG"
     gene            1541..2257
                     /locus_tag="VF516_26825"
     CDS             1541..2257
                     /locus_tag="VF516_26825"
                     /inference="COORDINATES: protein motif:HMM:NF024762.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEX8111384.1"
                     /translation="MGSPELERFAEVVACDDFPLDHAALLIGAWDYPERDLGGYRSQI
                     DAIAQFVEPAVARATGGIGRARAISDCLFDRLGFHGNTGDYYDPRNSFLCEVLDRRCG
                     IPISLSVLYLEVSRRVGVLAQGVNFPGHFLVRVAIEDAWLFIDPFSGGRVLASADLEA
                     LLRRTTTPDAVLEPNVIAAATKRQILARMLVNLAGIYGRNGDLPRSLDVLERLAVLEP
                     GNPRISRDLAQLRARVDSLN"
     gene            2345..3493
                     /locus_tag="VF516_26830"
     CDS             2345..3493
                     /locus_tag="VF516_26830"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX8111385.1"
                     /translation="MSGPGEPFAEQSVARQLDYCAFCPKMCRHACPVSTASGRETYIP
                     QVKMDRLNQLRLGHAGWTTEASDPLWACTGCRHCTMYCDHGNEPGLVLFTGRAIAAAR
                     GVSHPALAGYSERFRAREQRLSAQLAVMFEPHLATDGEIGYWPGCDAIDKSASDIAAA
                     LALFERVGLEGVRIVEAPQACAGYPLLAAGHRDLFRWHAGRVASALRGFGKVAINCSA
                     CLYALRAQYPAEGINLRTEIVSLADLLAPAAANLACPVTRRSVYYHDPCYHARYNGVV
                     EQPRRALSQLAELRELAWNRTDTECCGAGGLLPKTNPAIADQMARRRLAELQSTGGGM
                     VVTSCASCAFLLQRNAPPSVEVANLATAMAQLSETAFTPPASAADDDA"
     gene            3615..5267
                     /locus_tag="VF516_26835"
     CDS             3615..5267
                     /locus_tag="VF516_26835"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8111386.1"
                     /translation="MGESPIADVSTAVGSGRGVDPPDLVPALTIVSHPVTHRAGERYL
                     LDRVGAGGEVSLSRNAPDFTRPDRALGQPLGDPFVSRKPIRFAPGPGGGVRVIVDAGG
                     TPVVAGASLEGSAGFTADDLAHGVTLELAERVVLLLHQVDRRVQDAADPLGMLGASTA
                     IRRVRTAIERVVDLAVPVLIRGETGTGKELVARAIHDKSPRHDGPFVSVNVGAIPREI
                     AAAELFGSTRGAYTGASRDRDGLFRAAHGGTLFLDEVGEAPSEVQVMLLRVLETGEFY
                     PVGAERPVAIQVRLIAATDANLEDQIQHGQFKAPLLHRLAGFEVRIPPLRERREDIGL
                     LFYHFAREELEALGEGHRLSPQDPYARPWLPAHVAAQLVGYAWPGNIRELRNVTRQLV
                     IESRGLPQLRLDPRLAEQLGVPARPIDSGPPRPPSDPAIEVARPPSDPAIEVRRKPSD
                     VSEGELQDALRACAWDLKAAADRLRIPRPSIYDLIERSPDIRTAGDLGADEIAQCFQA
                     CAGDLDAMVAQLQVSRRALSRRLRELGLAGRARTGRPSSESH"
     gene            5838..6203
                     /locus_tag="VF516_26840"
     CDS             5838..6203
                     /locus_tag="VF516_26840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111387.1"
                     /translation="MTRNQLLPNYVKNWSGGVMFTVDGSGNITPTAQQNINRGETILT
                     QPDPACNTTFSGHVCARQNDVCQCSTLVGVSSGSHMAIGTGYTISSSVTVGSNFEIYA
                     VTDSGPVASATNGNIHINS"
     gene            complement(6249..9917)
                     /locus_tag="VF516_26845"
     CDS             complement(6249..9917)
                     /locus_tag="VF516_26845"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111388.1"
                     /translation="MQELREVELRIAVAEGVLSRAEADALGEEARRKKQSPLALLVAQ
                     GRLSEDSCQSILAEAMNDPAWRAAGRDASASTYTMQMEPAPSAEPPFPVAGWDRYTSV
                     RFLGQGGMGMVFLAIDTRLRREVAIKFVRGNNADHVRRLISEARNQARVSHERICKVY
                     EVDEVDGKVYIAMQYIAGKPLGRMADELTLEQKVTLVRGAAEGIHAAHTEGIIHRDIK
                     PSNIMVERMDDGELRPYVMDFGLARSVQDVGETLTGAVVGTPRYMAPEQAQGATSKLD
                     RRADVYSLGATLYHLVTGEPVVPGETIAEVIHNLIENEPRPLRAHDPGIPVDLEAIVL
                     KCLEKDRSARYDSARALADDLGRFLNGEPVIARPADTWYRMRKRLAKHRRLVAAAVVA
                     FAALVVAIGWAISTRIEAAQREQFARQFTEAVESIESTARYSAQSPLHDIRDDRTAIR
                     AQMAEIQTEIERGGAAASGPGHYALGRGYLALDDDVRAREHLAAAWTGGYREPRVAYA
                     LAQVMGHLYQQNLLAAERIEDKEAREAKKREIEWRYRDPALGFLSEAMRAQGAASPGY
                     AHAKDVAPPNYMAALVAYYEGRFDDALKHLKAIGRGLPWFYEAPKLRGDVLFARALGF
                     RNGGDSERARSDVEEGRQAYAAAASTGRSVPSIYKSLGEFEHTAMVMELYGHGDVTPV
                     ATRALDAAAQALATDPDYYEALVLDARVRRSMAEHQTIQGLNAEDLLRKALANAQRAV
                     GSSPARPEAQLEIARVYYQWGDYRNEHHEDPSEQFRQAVAASSAVPSAGRDSTYYVNL
                     GDTFSVWADYEDDHGMDGREHRGQSIDAYTQALRYAEAFHRKDQQRNAWAGLALNLYK
                     RATASRATAPDADLAQAITALGKAKLADRDNYVLYFYEGQIYEAIAQRTRVRGQSPGP
                     ALDKALAGYHRAIDINNKVPNLHNGAGAVFQALANVAWERGEDPEPALDQAQAEFLQA
                     TVVAPSQGFGYDNVGEVHVQRALFQRARGEDPSTSVKDAVDLLERAIDKIPNHPTFRA
                     DLAMAYTLLAEYQLDHGRDPRPSLDAADKALHDVLDSRDPQVALYHAETLGLRARLAA
                     RQGRGDARELERASQAFEQAVALAPENHDYALVFVRFCRALALFQRDSGGDPEPALQR
                     GLKLTARVLADPPRSPDALVLQASLTLARAQHIADAAGRRAEAERARQGFAAAIAINH
                     TLEKVWAGEIALAEQLAR"
     gene            complement(10079..10711)
                     /locus_tag="VF516_26850"
     CDS             complement(10079..10711)
                     /locus_tag="VF516_26850"
                     /inference="COORDINATES: protein motif:HMM:NF024802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8111389.1"
                     /translation="MSLTLHVGSKRYSSWSLRPYLALAHTGLAFETQVILLDRPTTRG
                     EIAAVNPAGRLPVLHHDGLVIWDSIAICEYLHELAPAAQLWPADRARRARARAISAEM
                     HAGFTALRSNMPMDLCADLSGQGHTPEALADARRVFEIWREALAASGGPFLFGGFTIA
                     DAMFAPVTTRFTTYGVELDATCRAYVDAIAALPAMVAWRRDAAAEPARRA"
     gene            complement(10784..>11063)
                     /locus_tag="VF516_26855"
     CDS             complement(10784..>11063)
                     /locus_tag="VF516_26855"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8111390.1"
                     /translation="RELENEIQRLVIQAESGHWIEITDLSPRLRKIEGTITKIAPQKG
                     TLREMMDQVERWLITEALREHGGNKTKTAVTLGITREGLHKKLAKFGV"
BASE COUNT         1516 a         4009 c         3861 g         1677 t
ORIGIN      
        1 gatgcggcag tggcgcgccg ccgggatgca gtcagatttc gtctgcgccg acggctggct
       61 cgtcgagggc ctggcgcccc gttgcgcgcg ttgctcgacc tgccggaccg cccagcgcgc
      121 gcacgcgtcg aggtcgggca gcgtatggcg catcggccgt caccagctgc gcgcggccat
      181 ccgccgcttg aaagtacacg ccggccggag gcgcgccctg accccctccg atgaccctgc
      241 cgccgcagaa gtgcacgacc gtaggcttgt gcttgacgga tcatccaggc tgtccctgtt
      301 caggacaccc cgggacaccc ccggctcagc cgcgcttctt gccgaagaac ggcaggtagt
      361 ggcgcaggct gcgcagccgc tcgaacgcct gggccgcggc gccctccccg cgcggcgccg
      421 gctcgagccg cggcggctcg tcgggcaaga gctcggcgag cggcgtcacg tcggagagct
      481 cggcgatgat gccgcccagg ccgagctcgg cccgcgcctc ggatacgctg cgcgacagca
      541 cgctgtggag gtccctgcgc agctggacct tcttgagcgc ggtgccgatc tggcgcgcca
      601 tctcgcgggt cgacgggaac cgatcgtcga tctcggcgga cagcgcgcgg tggatgatct
      661 gggcgaacgc cggcggcacg tcgggccgca gcggccgcag cggctggacc aggcagtcgc
      721 gcagccggca gtacgcctcg tagtcggtct cgccgtcgaa cagcttgcgc ccgaccagcg
      781 cctcccacag caccgagccg cacgcgaact gatccgacgc cgggctcggc cggccgccgg
      841 tcacgatctc gggcgcgagg tacgacatct tgcccttgac gatgccgggg tcggtcgtct
      901 cctgggcgcg gtcgggcgcg aacgcgagcc cgaagtcgat cagcttgacc acgccgcgcg
      961 tggtgagcag cacgttgtgc ggcgacacgt cgcgatggat gatcggcagc ggcgcgccgt
     1021 cggggccggt ccgctcgtgg gcggcggcca gcgcgcgcag cacgccgacg gcgaccgcgg
     1081 cgaccagctc ccagcgggtc ggctcgttgc gtgcgacgtg ccacttgatc cagctgccga
     1141 ggtcgacgcc gtcgatccac tccagcacca tgtagtaatg gccgcggtcg tggacgaagt
     1201 cgtggacctc cgagatgttg ggggacttca gcaccgcgcc gacccgggcc tcctcgacga
     1261 acatcgcgac gtacggaggc tgggtcgtca ggttggcgtg catctgcttg atcgcgacgt
     1321 cgcgcacgaa gccggagtcg ccgcgcaccc ggccgcgcca gacatcggcc atgccgccgc
     1381 gaccggcgac ctcgatcagc tcgtagcggc cgccgacgat cagacctggg cgcggctcct
     1441 cggccatcgg ccggccaagc atcgcataca ttcccctcgg agattgcccg agcgggagat
     1501 cattcacggg cccggggggt ggatgctaga ttcccgttcg atgggcagtc ccgagctcga
     1561 gcggttcgcc gaggtcgtcg cgtgcgacga cttcccgctc gaccacgccg cgctgctgat
     1621 cggcgcatgg gactatccgg agcgcgatct cggcggctat cgcagccaga tcgacgcgat
     1681 cgcacagttc gtcgagcccg cggtcgcgcg cgccaccggc ggcatcggcc gggcccgcgc
     1741 gatcagcgat tgcctgttcg accggctcgg gttccacggc aacaccggcg actactacga
     1801 tccgcgcaac agcttcctgt gcgaggtgct cgatcgccgg tgcggcatcc cgatctcgct
     1861 gtcggtgctc tacctggagg tctcgcgccg ggtcggcgtg ctggcgcagg gcgtgaactt
     1921 ccccggccac ttcctggtcc gcgtcgcgat cgaggacgcc tggctgttca tcgatccgtt
     1981 cagcggcggc cgcgtgctcg cctccgccga cctcgaggcg ctgctccggc gaaccacgac
     2041 tccggatgcc gtgctcgagc cgaacgtgat cgccgcggcg accaagcgcc agatcctcgc
     2101 acgcatgctg gtcaatctcg ccgggatcta cgggcgcaac ggcgatctgc cgcgctcgct
     2161 cgacgtgctg gagcggctcg ccgtgctcga gcccggcaac ccgaggatct cgcgcgatct
     2221 cgcgcagctg cgcgcgcgcg tcgacagcct gaattgaccg cggccggacc tgacgcgcgg
     2281 ccgggctgac gtatagtggc cgcgatgtgc gagcgagcac gcgagagcca cgccgcgggc
     2341 gaccatgtcc gggcccggtg aaccgttcgc ggagcagtcg gtcgcgcgcc agctcgacta
     2401 ctgcgcgttc tgccccaaga tgtgccggca cgcctgcccg gtatcgacgg cgagcggccg
     2461 cgagacctac atcccgcagg tcaagatgga ccggctcaac cagctgcggc tcggccacgc
     2521 cggctggacc acggaggcca gcgacccgct gtgggcgtgc accggctgcc ggcattgcac
     2581 gatgtactgc gatcacggca acgagcccgg cctggtgctg ttcaccggcc gcgccatcgc
     2641 cgccgcccgc ggcgtgtcgc atcccgccct cgccggctac agcgagcggt tccgcgcccg
     2701 cgagcagcgg ctcagcgccc agctcgcggt gatgttcgag ccccacctcg cgaccgacgg
     2761 cgagatcggc tactggccgg gctgcgacgc gatcgacaag tcggcgtcgg acatcgcggc
     2821 cgcgctcgcg ctgttcgagc gggtcggcct ggagggcgtc cggatcgtcg aggcgccgca
     2881 ggcgtgcgcc ggctatccgc tgctcgccgc cggccaccgc gacctgttcc gctggcacgc
     2941 cggccgggtg gcgtcggcgc tgcgcgggtt cggcaaggtc gcgatcaact gctcggcgtg
     3001 cctgtacgcg ctgcgcgcgc agtacccggc cgagggcatc aacctgcgca ccgagatcgt
     3061 ctcgctcgcc gacctgctcg ccccggccgc cgccaacctg gcgtgcccgg tgacccgccg
     3121 ctcggtctac taccacgacc cgtgctacca cgcgcgctac aacggcgtcg tcgagcagcc
     3181 gcgccgcgcg ctgtcccagc tcgccgagct ccgcgagctg gcgtggaacc ggaccgacac
     3241 cgagtgctgc ggcgcggggg gccttctgcc caagaccaac cccgcgatcg ccgaccagat
     3301 ggcccggcgc cggctcgccg agctccagtc gaccggcggc ggcatggtcg tcacctcgtg
     3361 cgcgagctgc gcattcctgc tccagcgcaa cgccccaccg tcggtcgagg tcgccaacct
     3421 cgccaccgcg atggcccagc tgtccgagac cgcgttcacc cccccggcga gcgccgccga
     3481 cgacgacgcc tgacaggcct gacaggccgc tcgccgccgc gcacctgcca cgacatcacc
     3541 tgcgaggtgg gtccagctac gacgaatcgt ttgtgacgag gaatgtgctg tatacccact
     3601 aggcctagcg cgacatgggt gagtcaccga tcgcggacgt ctccaccgcc gtcgggtcag
     3661 gacggggcgt cgatccgccc gaccttgtcc cggcgctgac cattgtctcg catccagtca
     3721 cccaccgcgc cggcgagcgc tacctgctcg atcgcgtcgg cgcgggcggc gaggtcagcc
     3781 tgtcgcgcaa cgcgcccgac ttcacgcggc ccgaccgggc gctcggccag ccgctcggcg
     3841 atccgttcgt cagccgcaag ccgatccggt tcgcacccgg ccccggcggc ggggtccgcg
     3901 tgatcgtcga tgcgggcggc acaccggtgg tcgcgggggc ttcgctcgag ggcagcgccg
     3961 ggttcaccgc ggacgacctc gcgcacggcg tcacgctgga gctcgccgag cgcgtggtgc
     4021 tgctcctgca ccaggtcgat cgccgggtcc aggacgccgc cgatccgctc ggcatgctcg
     4081 gcgcgagcac ggcgatccgg cgggtccgca ccgcgatcga gcgcgtcgtc gacctcgcgg
     4141 tgccggtgct gatccgcggg gagaccggga ccggcaagga gctggtcgcg cgcgcgatcc
     4201 acgacaagag cccgcgccac gacggcccgt tcgtcagcgt gaacgtgggc gcgatcccgc
     4261 gcgagatcgc cgccgccgag ctgttcggct cgacccgcgg cgcctacacc ggcgcgtcgc
     4321 gcgaccgcga cgggctgttc cgcgccgcgc acggcggcac gctgttcctc gacgaggtcg
     4381 gcgaggcccc gtccgaggtc caggtcatgc tgctccgggt cctggagacc ggcgagttct
     4441 acccggtcgg cgccgagcgg cccgtggcga tccaggtccg gctgatcgcc gcgaccgacg
     4501 ccaacctgga ggaccagatc cagcacggcc agttcaaggc gccgctgctc caccgcctgg
     4561 ccggcttcga ggtccggatc ccgccgctgc gcgagcggcg cgaggacatc ggccttctgt
     4621 tctatcactt cgcccgcgag gagctggagg cgctcggcga gggccaccgg ctgtcgccgc
     4681 aggatcccta cgcccggccg tggctgcccg cgcacgtcgc ggcccagctg gtgggctacg
     4741 cctggcccgg caacatccgg gagctgcgca acgtgacccg gcagctcgtg atcgagagcc
     4801 gcggcctgcc ccagctgcgg ctcgatccgc gtctcgccga gcagctcggc gtgccggcgc
     4861 ggccgatcga ctccggaccg ccgcggccgc cctcggatcc ggcgatcgag gtggcgcggc
     4921 cgccctcgga cccggcgatc gaggtccggc gcaagccgtc cgacgtgtcg gagggcgagc
     4981 tacaggatgc gctccgggca tgcgcgtggg acctcaaggc cgcggccgac cggctgcgca
     5041 tcccgcggcc gtcgatctac gacctgatcg agcgcagccc cgacatccgg accgccggcg
     5101 acctcggcgc cgatgagatc gcgcagtgct ttcaagcctg tgccggcgat ctcgacgcga
     5161 tggtcgcgca gctccaggtg tcgcgccgcg cgctcagccg ccggctgcgc gagctcgggc
     5221 tcgccggccg ggcgcggacg ggccgcccga gctccgagtc ccactgacgc tcaccgggtt
     5281 gccccaggcg gacagggata accgaccgac atccgacgcc attcccgcga tgccccgaac
     5341 tcgtccatcg agctcgctcc cgcccgctcc cccggcatga atcgtccctg atctggacga
     5401 gatcctggca tcgctcccct gccccgccca cgactcccgg actggcaatc gttggccggt
     5461 cggtcatccc tgttcgtctg cggcccccga accgcgtggt gcaccatggt gcaaccgatg
     5521 gaccgatcat cgggcgtgtc gcgaccggcc gctcgccccg tccatgacgg gcttggccga
     5581 gccgccggcc ggggccgcgc gcgggtgacg cagcgtcgac atgtcgagcg cgccggttcg
     5641 acatgtcgag ccaccctcgg tgcgccggac ggcgtcccag ccgaccgtta tgccagtgtt
     5701 cgaagttttc catcagcctg gcatgtcgct tgtaccaccg agcttccggg cgcggtcggg
     5761 taagggaacg cgctggatct ggtctcaggc aatgactgcc tgagatgtct catcgaggga
     5821 aagttaggag tgtaggtatg actcggaacc agcttttacc gaactatgtg aagaactggt
     5881 cgggcggcgt gatgttcacc gtggatggca gcgggaacat cacgcccaca gcccagcaga
     5941 acatcaatcg tggggagacg atcctgactc agccggatcc cgcgtgcaac acgacgttca
     6001 gtgggcatgt gtgcgcacgg cagaacgacg tgtgccagtg ctcgacgctg gtcggcgtgt
     6061 ccagcggcag ccacatggcg atcggcaccg ggtacacgat cagctcgagt gtgaccgtcg
     6121 gtagcaactt cgagatctac gcggtcacgg actcgggccc ggtcgccagc gccaccaacg
     6181 gcaatatcca catcaacagc tgagtccgcc gactgggtac cagtcggtcg agcgcggcga
     6241 ggccgctgct acctggcgag ttgctcggcc agcgcgatct cgcccgccca caccttctcc
     6301 agggtgtggt tgatcgcgat cgcggcggcg aagccctgcc gcgcacgttc ggcctctgcg
     6361 cgtcgaccgg cggcatcggc gatgtgctgg gcgcgcgcga gtgtcaagct ggcctgcagg
     6421 acgagcgcat ccggggatcg cggtgggtca gccagcaccc gcgcggtcag cttcagcccg
     6481 cgctgcagcg caggctcggg atcaccgccc gagtcgcgct ggaacagggc taaggcacgg
     6541 cagaaccgga caaatacgag cgcatagtca tggttctcag gtgcgagtgc gaccgcttgt
     6601 tcgaacgcct gggacgcccg ctcgagctcg cgagcgtcgc cacggccctg tcgcgctgcc
     6661 agccgggcgc gtagtcccag cgtctcggcg tggtagagcg cgacctgagg atcgcgggag
     6721 tccagcacgt cgtggagcgc cttgtccgca gcgtccaggc tggggcgcgg gtcgcgcccg
     6781 tggtccagct ggtactcggc aaggagggtg taggccatgg cgaggtcggc ccgaaaggtc
     6841 ggatggttcg ggatcttgtc gatcgcccgc tccaggagat ccaccgcgtc cttcacgctc
     6901 gtgctcggat cctcgccccg cgcccgctga aacagcgcgc gctggacatg gacctccccc
     6961 acgttgtcgt agccaaaccc ctggctggga gcgaccacgg tggcctgcag gaactccgct
     7021 tgcgcctgat ccagtgctgg ctccggatcc tctccacgct cccaggcaac gttcgccagc
     7081 gcctggaaca ccgcaccggc accgttatgg aggttcggaa ccttgttgtt gatgtcgatc
     7141 gctctgtggt atccggccag ggccttgtcg agggcaggac cggggctctg cccgcgcacg
     7201 cgggtgcgct gtgcgatcgc ctcgtagatc tggccctcgt agaagtacag aacgtagttg
     7261 tctcgatcag ccagcttggc cttgcccagt gcagtgatcg cctgcgcgag gtcggcgtcc
     7321 ggggccgtcg cgcgggatgc tgtcgcccgc ttgtacaggt tcagggccag gccggcccag
     7381 gcattgcgct gctgatcctt gcggtgaaat gcctccgcat atcgcagcgc ctgcgtgtag
     7441 gcatcgatcg attggccccg gtgttcgcgg ccatccatgc cgtgatcatc ctcgtagtcg
     7501 gcccacaccg agaacgtgtc gcccaggttg acgtagtacg tgctgtctcg gccggcggac
     7561 ggaactgcgc tggatgcggc gaccgcctga cgaaactgct cgctcggatc ctcatggtgt
     7621 tcgttgcgat aatctcccca ctggtaatag acacgcgcga tctccagctg cgcctcgggc
     7681 cgcgcgggag atgacccgac cgcgcgctgg gcgttggcga gcgccttgcg aagcaagtcc
     7741 tcggcgttca ggccctggat cgtctggtgc tccgccatgc tgcgccggac gcgggcgtcg
     7801 aggacgagcg cctcgtagta gtcagggtcg gtcgcgagcg cctgcgccgc cgcgtcgagc
     7861 gcccgggtgg ccaccggcgt cacatcgccg tggccgtaga gctccatgac catggcggtg
     7921 tgttcgaact caccgagtga cttgtagatc gacggaacgc tccggccggt gctcgctgca
     7981 gcggcatacg cctggcggcc ttcctcgacg tcgctgcgcg cccgctcgct gtcgccgccg
     8041 ttgcggaacc ccagggcccg cgcgaacagg acatctccgc gcagcttcgg cgcctcgtag
     8101 aaccacggca ggccacggcc gatcgccttc aggtgcttca gcgcatcgtc gaaccggcct
     8161 tcgtagtacg ccaccagcgc ggccatgtag ttcggcggtg cgacgtcctt ggcatgcgcg
     8221 tatcctgggg acgcagcgcc ctgggcgcgc atggcctccg agaggaagcc gagcgccgga
     8281 tcgcggtacc gccactcgat ctcgcgcttc ttggcctcgc gggcctcctt gtcctcgatc
     8341 cgctcggcgg cgagcaggtt ctgctggtag aggtgcccca tcacctgcgc cagcgcatac
     8401 gcgacgcgcg gctcgcggta gccgccggtc caggccgccg cgaggtgctc acgcgcccgg
     8461 acgtcgtcgt cgagcgcgag gtagccgcgg ccgagcgcgt agtgtccggg gccggacgcc
     8521 gccgcgccgc cgcgctcgat ctcggtctgg atctcggcca tctgcgcgcg gatcgccgtg
     8581 cggtcgtccc ggatgtcgtg cagcggtgac tgcgccgagt agcgcgccgt cgactcgatg
     8641 ctctcgactg cctcggtgaa ctgccgcgcg aactgctcgc gctgggcggc ctcgatccgg
     8701 gtgctgatcg cccagccgat cgcgaccacg agcgccgcga acgccaccac cgccgccgcg
     8761 accagccgcc ggtgcttggc gagccgcttg cgcatccggt accaggtgtc ggcggggcgg
     8821 gcgatgaccg gctcgccgtt gaggaaccgg ccgaggtcgt cggccagcgc gcgcgccgag
     8881 tcgtagcggg ccgagcggtc cttctccagg cacttgagca cgatcgcctc gagatcgacc
     8941 gggatgccgg ggtcgtgggc gcgcagcggg cggggctcgt tctcgatcag gttgtggatc
     9001 acctcggcga tggtctcgcc cgggaccacc ggctcgccgg tgaccaggtg atacagcgtc
     9061 gcgccgaggc tgtagacgtc cgcgcggcgg tcgagcttgg aggtcgcgcc ctgcgcctgc
     9121 tcgggggcca tgtagcgcgg cgtgcccacc accgcgccgg tgagcgtctc gccgacgtcc
     9181 tgcaccgagc gcgcgagacc gaagtccatg acgtagggcc ggagctcgcc gtcgtccatg
     9241 cgctcgacca tgatgttcga tggcttgatg tcgcggtgga tgatgccttc ggtgtgggcg
     9301 gcgtggatgc cctcggcggc gccgcgcacc agcgtcacct tctgctccag ggtgagctcg
     9361 tcggccatcc ggccgagcgg cttgcccgcg atgtactgca tcgcgatgta gaccttgccg
     9421 tcgacctcgt cgacctcgta gaccttgcag atccgctcgt ggctgacccg ggcctggttg
     9481 cgcgcctcgg agatcagccg ccggacgtgg tcggcgttgt tgccgcgcac gaacttgatc
     9541 gccacctcgc ggcgcagccg ggtgtcgatc gcgaggaaca ccatgcccat gccgccctgg
     9601 ccgaggaacc ggacgctggt gtagcggtcc cagcccgcga ccggaaacgg cggctcggcg
     9661 gacggcgccg gctccatctg catcgtgtac gtgctcgccg atgcgtcccg gccggcggcg
     9721 cgccacgccg ggtcgttcat cgcctcggcg aggatcgact ggcagctgtc ctcggacagc
     9781 cggccctgcg cgacgagcag cgccagcggg ctctgcttct tgcggcgcgc ctcctcgccg
     9841 agcgcgtcgg cctccgcgcg cgagagcaca ccctcggcca cggcgatcct caactccacc
     9901 tcgcgcaact cctgcatcgg cagaagcata ccctcggcgc gtgaaccacc gtcgctcgca
     9961 gccgccgtga ggatcagcta gcgatccgcg ctgcagcccc gccgcgcgct gcggcagtgt
    10021 acgccacgcg ctgccactgt gcgccggcga tcgccggccg ttgccggagc cgtcgcgatc
    10081 aggctcgccg ggccggctcg gcggccgcgt cgcgccgcca cgcgaccatc gcgggcagcg
    10141 ccgcgatcgc gtcgacgtag gcgcgacagg tcgcgtcgag ctcgacgccg taggtcgtga
    10201 accgcgtggt caccggcgcg aacatcgcgt cggcgatcgt gaacccgccg aacaggaacg
    10261 gcccgccgct cgcggccagc gcctcgcgcc agatctcgaa cacccggcgg gcgtcggcga
    10321 gcgcctccgg agtgtggccc tggcccgaca ggtcggcgca caggtccatc ggcatgttgc
    10381 tgcgcagcgc ggtgaacccg gcgtgcatct cggcgctgat cgcgcgggca cgggcgcgcc
    10441 gcgcccgatc ggccggccac agctgggccg cgggagcgag ctcgtgcagg tactcgcaga
    10501 tcgcgatcga gtcccagatc accaggccgt cgtgatggag caccggcagg cgtccggccg
    10561 ggttgaccgc ggcgatctcg ccgcgcgtgg tcgggcggtc gagcaggatg acctgggtct
    10621 cgaacgcgag cccggtgtgc gccagcgcga ggtacggccg gagcgaccag gacgagtaac
    10681 gcttgctgcc gacgtggagc gtgagcgaca tgccggcact ctatcagcga tgcgccggcc
    10741 cgagcgcaca gcgcgccgcg acgcggccgg tacagcgccc gggctacacg ccgaacttgg
    10801 cgagcttctt gtgcagcccc tcgcgggtga tcccgagggt gaccgcggtc ttggtcttgt
    10861 tgccgccgtg ctcgcgcagc gcctcggtga tcagccagcg ctcgacctgg tccatcatct
    10921 cgcggagcgt gcccttctgc ggcgcgatct tggtgatcgt gccctcgatc ttgcgcagcc
    10981 gcggcgacag gtcggtgatc tcgatccagt ggccggactc ggcctggatc accaggcgct
    11041 ggatctcgtt ctcgagctcg cgg
//
LOCUS       DASVOR010001411         1516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_511159,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001411 DASVOR010000000
VERSION     DASVOR010001411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511159"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF516_26860"
     CDS             complement(<1..612)
                     /locus_tag="VF516_26860"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8111391.1"
                     /translation="MLEDAHALVIGISRYLHAPELRTTQDAQDVAAALADPACCGYPP
                     AAVRVLLDGDATRAAILAALDALAHDTREASTVFFYYSGHGARAAGGSNASYYLVPVD
                     AAITSRDDLDRTAISNAELTARLRAIPAGRLTVVLDCCRAADMAELRLDEAIAPLAEG
                     RGRVVLAASRDAAYSMPGDRDSTMTGYLIDGLRGAASGVGGVIR"
     gene            742..>1516
                     /locus_tag="VF516_26865"
     CDS             742..>1516
                     /locus_tag="VF516_26865"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111392.1"
                     /translation="MSRPDGDATLTAMDRSGEPPRPIAELGPGARIGRYVIERRIGAG
                     GMGVVYAARDSELARDVALKIVRPRAEVDAMQARLRREAKAMARLSHPNVVPVFDIGL
                     HEGQLFIAMELIAGETLRSWVAQPRPWRSVVALFQKVGRGLEAAHAAGLLHRDFKPDN
                     VVVGPGDEPRITDFGLARELDDRAGSAAALAEGSDSELSLITRTGSLAGTPAYMAPEA
                     LLGRQSGAAADQFSFCVSLFEVLHGARPFRPAAPRSEALI"
BASE COUNT          185 a          504 c          638 g          189 t
ORIGIN      
        1 ccggatcacg ccgccgacgc cgctggccgc gccgcgcagg ccgtcgatca ggtagccggt
       61 catcgtgctg tcgcggtcgc ccggcatcga gtaggccgcg tcgcgcgacg ccgcgagcac
      121 gacccggccc cggccctcgg cgagcggcgc gatcgcctcg tcgagccgca gctcggccat
      181 gtcggcggcg cggcagcagt cgaggacgac ggtgagccgg ccggcgggga tcgcgcgcag
      241 gcgggcggtg agctcggcgt tcgagatcgc cgtgcggtcg aggtcgtcgc gcgaggtgat
      301 cgcggcgtcg accgggacca ggtagtagct ggcgttgctg ccgccggccg cgcgcgcgcc
      361 gtgccccgag tagtagaaga acacggtcga cgcctcgcgg gtgtcgtggg cgagcgcgtc
      421 gagcgcggcg aggatcgcgg cgcgggtggc gtcgccgtcg agcaggacgc ggacggcggc
      481 cggtggatag ccgcagcagg cggggtcggc gagcgcggcg gcgacgtcct gcgcgtcctg
      541 ggtggtgcgc agctcgggtg cgtggaggta gcgcgagatg ccgatcacca gggcgtgcgc
      601 gtcttcgagc atcgagcggc ccgagcatac cccggcgacg gcggcggccg gtgggccgtc
      661 gcggagacac tgaacatgga acactgcgcg tcacggccgg ccggcgctcg tctggcggcc
      721 gcgatcggct atcctcgctg ggtgtcgagg ccggacggcg acgccacgct gaccgccatg
      781 gatcgatccg gcgagccgcc caggccgatc gccgagctcg ggccaggtgc gcggatcggg
      841 cgctatgtga tcgagaggcg gatcggcgcc ggcgggatgg gtgtggtgta cgcggcgcgg
      901 gacagcgagc tggcgcgcga cgtggcgctg aagatcgtgc ggccgcgcgc cgaggtcgac
      961 gcgatgcagg cgcggctgcg ccgcgaggcc aaggcgatgg cgcggctgtc gcacccgaac
     1021 gtggtcccgg tgttcgacat cggcctgcac gagggccagc tgttcatcgc gatggagctg
     1081 atcgccggcg agacgctgcg gtcgtgggtc gcgcagccgc ggccgtggcg cagcgtggtg
     1141 gcgctgttcc agaaggtcgg gcgcgggctg gaggcagcgc acgcggcggg gctgctccac
     1201 cgcgacttca agcccgacaa cgtggtggtc gggccgggcg acgagccgcg gatcaccgac
     1261 ttcgggctcg cgcgcgagct cgacgacagg gccggctcgg cggccgcgct cgccgagggc
     1321 agcgacagcg agctgtcgct gatcacccgg accggcagcc tggcgggcac gccggcgtac
     1381 atggcgccgg aggcgctgct ggggcgccag agcggcgcgg cggccgatca gttcagcttc
     1441 tgcgtgtcgc tgttcgaggt gctgcacggc gcgcggccgt ttcgccccgc ggcgccgcgg
     1501 tcggaggcgc tgatcg
//
LOCUS       DASVOR010001412         2292 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_452649,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001412 DASVOR010000000
VERSION     DASVOR010001412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452649"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VF516_26870"
     CDS             complement(<1..403)
                     /locus_tag="VF516_26870"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8111393.1"
                     /translation="MSVKPPLLDDEAHDPIDTLRWALVPLRITIAIGFVMHGESKLVR
                     GPEHFAHILEALGVPMSHVAAWLTILVEIIGGVSLLLGAYREAGGGRARHPAGRRGRR
                     AAAGAPLAAAGRAGVAAGRSEQAAGPAAVHPQ"
     gene            complement(672..>2292)
                     /gene="sppA"
                     /locus_tag="VF516_26875"
     CDS             complement(672..>2292)
                     /gene="sppA"
                     /locus_tag="VF516_26875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX8111394.1"
                     /translation="TAGPASLLGTPDHIERVELSGAIGARQLSALVVRLRAIARDPSA
                     RAVVVTFDGASAGWAALEELRDELVRIRAAGKKLFAYMVSGTGRDYFVATAADKIYVD
                     PAGGLRLVGMAGTTMYFRGAFDWLGVVPQFEKIAEYKSAPEQFTESRPSAAASRMRGE
                     LFDSLWDRWVAAVAAARHLSRDQLLALVDGGPYTAGDLAHDTRLVDAVAAPDKISELV
                     IREIGEILPVTAPAADRPDRWQLPGIAVIYVDGDITDGKSQTVPVIGETLTGADSVIQ
                     ALSEARSDPRIGAIILRIDSPGGSAVASELIAREVFATRGVKPILCSMSNVAASGGYF
                     VAAGCDAIFAEPMTITGSIGIFYGKFDVAGLAGKLGITTDTYKRGKHADLESMFRPYT
                     DEERAVLQGNLRYMYGRFVDAVAEGRGVQPDAVDAVGRGHVWSGAQAMPIKLIDRFGG
                     LGDAIDEAKRRIGLAATDKIQLRELPDQPASLLGALGVLIGVSGGVSGERTLGLSELP
                     ALRQLLQGVPASLLVDQSKPQARLPFTLSWQ"
BASE COUNT          340 a          835 c          804 g          313 t
ORIGIN      
        1 gctgagggtg aacggcagcc gggcctgcgg cttgctctga tcgaccagca gcgacgccgg
       61 cacgccctgc agcagctaac ggagcgccgg cagcggcgcg gcgaccgcgt cgaccagccg
      121 ggtgtcgagc acgaccgccg ccggcctcac ggtatgcgcc caggagcagg ctgactcctc
      181 cgatgatctc gacgagaatc gtgagccatg cggccacgtg cgacatcggg accccgagcg
      241 cctcgaggat gtgcgcgaag tgctcgggtc cgcgcacgag cttcgactca ccgtgcatca
      301 cgaatccgat ggcgatcgtg atgcgcagcg ggaccagtgc ccagcgtagg gtgtcgatcg
      361 gatcgtgagc ttcgtcgtcc agaaggggtg gcttgaccga cacgatcgcc gagcgcccgg
      421 gcccgacggc caagccccgc cgagcgctaa cgcgcgccaa cctgctgaag ccgaaccgcg
      481 ttcctgacga accgcgacgg ggtcggtggt gcccaatgcc gtgttgaacg cgagcagccg
      541 tcaggattgg gtgctcgcgg tctgacgcat cgccacctcg cgatcgtcgc gcgagacagg
      601 ttctgcggcg ggcgcgtgcg cacgatccgt gtgttccgaa ccatggcgcc atgctggatc
      661 ggaggcggcg atcactgcca gctgagggtg aacggcagcc gggcctgcgg cttgctctga
      721 tcgaccagca gcgacgccgg cacgccctgc agcagctggc gcagcgccgg caactcggac
      781 aggccgagcg tccgctcgcc ggacacgccg ccggaaacac cgatcaacac gccgagcgca
      841 ccgagcaggc tcgccggctg atccggcagc tcgcggagct ggatcttgtc ggtcgcggcc
      901 aggccgatcc gccgcttggc ctcgtcgatc gcgtcgccca ggccgccgaa ccggtcgatc
      961 agcttgatcg gcatcgcctg cgcgccgctc cacacgtggc cgcggccgac cgcgtcgacc
     1021 gcgtcgggct ggacgccgcg gccctcggcg accgcgtcga cgaaccggcc gtacatgtag
     1081 cgcaggttgc cctgcagcac cgcgcgctcc tcgtcggtgt acggccggaa catgctctcg
     1141 agatcggcgt gcttgccgcg cttgtaggtg tcggtcgtga tcccgagctt gccggcgagg
     1201 ccggcgacgt cgaacttgcc gtagaagatg ccgatcgagc cggtgatcgt catcggctcg
     1261 gcgaagatcg cgtcgcagcc ggcggcgacg aagtacccgc ccgacgccgc gacgttgctc
     1321 atcgagcaca ggatcggctt gaccccgcgg gtcgcgaaca cctcgcgcgc gatcagctcg
     1381 gacgcgaccg cgctgccacc gggcgagtcg atgcgcagga tgatcgcgcc gatccgcgga
     1441 tcgctgcgcg cctcgctcag cgcctggatc acggagtcgg cgccggtcag ggtctcgccg
     1501 atcacgggca cggtctgcga cttgccatcg gtgatgtcgc cgtcgacgta gatcaccgcg
     1561 atgccgggca gctgccagcg gtccggccga tcggccgccg gcgcggtcac cggcaggatc
     1621 tcgccgatct cgcggatcac cagctcgctg atcttgtcgg gcgcggcgac cgcgtcgacc
     1681 agccgggtgt cgtgggcgag atcgcccgcg gtgtacggcc cgccgtcgac cagcgcgagc
     1741 agctggtcgc gcgacaggtg gcgcgccgcg gcgaccgcgg cgacccagcg gtcccacagc
     1801 gaatcgaaca gctcgccgcg catccgggac gccgccgcgc tcggcctgga ctcggtgaac
     1861 tgctcgggcg cgctcttgta ctcggcgatc ttctcgaact gcggcaccac gccgagccag
     1921 tcgaacgcgc cgcggaagta catcgtggtg cccgccatcc cgaccagccg cagcccgccg
     1981 gccgggtcga cgtagatctt gtcggccgcg gtcgcgacga agtagtcgcg cccggtcccc
     2041 gacaccatgt aggcgaacag cttcttgccg gcggcgcgga tccggaccag ctcgtcgcgc
     2101 agctcctcca gcgccgccca gcccgcgctc gcgccgtcga acgtcaccac cacggcgcgc
     2161 gcgctgggat cgcgggcgat cgcgcgcagc cgcacgacca gcgccgacag ctggcgcgcg
     2221 ccgatcgcgc ccgacagctc gacccgctcg atgtggtcgg gcgtgccgag cagcgacgcc
     2281 ggtccggcgg tc
//
LOCUS       DASVOR010001413         5315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_190064,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001413 DASVOR010000000
VERSION     DASVOR010001413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190064"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..663)
                     /locus_tag="VF516_26880"
     CDS             complement(<1..663)
                     /locus_tag="VF516_26880"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8111395.1"
                     /translation="MSILGPIPVAVFAWIAGGAAVLAIGAYIIKMRRRRFEVPFSRLW
                     QRVLEQRDANALWKQLKRLLSLLLILFIIAIMLFAALDPTLGVTDRKARSVVLLFDAS
                     ASMKAMDGNDKGDQSRLDAAKARAKHLIDGMGGGDVAMIMKVDGQATPMSRFSGDAPM
                     LDKIVDGIAASDTPADLTRALGAAADALRDRPNPLIVLVSDGAFPEQQLSLVRWTPPA
                     PPG"
     gene            complement(737..1693)
                     /locus_tag="VF516_26885"
     CDS             complement(737..1693)
                     /locus_tag="VF516_26885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8111396.1"
                     /translation="MARSAAALRPSDRPAAAGPVHRARDRTALFDDRFLKTLEHLHMV
                     SRKVFAGNLRAERRTRKVGSGIEFADHRTYARGDDFRYIDWNLYGRLDKLLLRLFEEE
                     EDLHIYILIDVSDSMAIGTPLPKLHYAMQVGAALAYVGLANLDRVAIIPFAGKLIDRL
                     PPSRGKNRIFRVFEFLRNCDIGGQTDLAECMKTFVGQNKRRGLAVLISDFYDPVGFEQ
                     GLNTLRYNKFEPFVLQVYDKREAEPQLHGDLALVDCETGDTREVTVSKSLLEAYQREH
                     ERYCKELEAYCTKYAMAFFRTHTSIPFDELVLRIFRSGGFLR"
     gene            complement(1699..2700)
                     /locus_tag="VF516_26890"
     CDS             complement(1699..2700)
                     /locus_tag="VF516_26890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8111397.1"
                     /translation="MTQAAAARDVETTVASFTTDLQRLRAEISTMIVGQEGIIEGVLM
                     CLLGGGHALLEGVPGLGKTMLVRTLAETIHATFSRIQFTPDLMPADIVGTNVITEDHG
                     IKRFEFQKGPIFANILLADEINRATPKTQSALLEAMSEGSVTVAKQTYQLEKPFFVLA
                     TQNPLEMEGTYPLPEAQLDRFFFKLVVEYPGKEDLHTILDRTTTDHLPSPQAVIEKAR
                     ILEMRELVRKVPLARQIQDYAVRVVLATHPENPEATAMSKKFMRYGASPRGLQAIILA
                     AKIRALLEGRYAVAIDDIRHVAPPALRHRMIMNFEGEAEGVRTDAIIAEILKATREA"
     gene            complement(2709..5057)
                     /locus_tag="VF516_26895"
     CDS             complement(2709..5057)
                     /locus_tag="VF516_26895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111398.1"
                     /translation="MIARARRRIRGQRALDGATTATILAAAAALAVVFSIRIEIVERA
                     AGLALLAGTAGIIVLGAVIAAVRRIDDEHVARRIDRASNLSDRLSTAVAFSRAGVLPA
                     DDETHQLMHAAIRDGVRAVPRADVARATPFTAPTDLRAALGFLAISALAAGLAIPTVD
                     RAPHLYRVEPDHAAAGDQVVLIGVNLMNGIAHPLASLPPRAWLDASLGAPGVPAEATA
                     PVAPVTAPQTAPMIRGFIPSDGQVTLGAGKDHPARVLDWAADRILVQIPDRAPIGPTT
                     LTAWIGDRAVGPLAFTVIDKKDPRYHKADSVVLDPDDRAYLDALLNEIKAVAKRDQVP
                     ELEDFAKQVEQLLQDAELGKISKEKLLEALAKAETKLEQHAEPDQADVDKQLAELGKQ
                     LSKDQLTKSLGDALQKTELDKAQQELEKLAEKLDKNELSEQQQQELAKQLEKAAKQLD
                     DKQQQQQKLNQQKQQKQQKLENDIRRLEKKKQEAKTDQERLDAERQLAKLKDELKKLD
                     KDAEDKEQSEQRQALKRLSRDLEKAAEDMQKKDGQKDKQNQEQQDQQGQKQQASQKLK
                     DAARETGRVDQDRRKQATQKKMSSQMEDLREAMRRAKQKGGKDGKDPFGKQNKNQDFA
                     QRARGQRGQSQAWKPGQGQQPGQGQPGQGGQGQGQPQASNEWGVGHDDNLVGDPTQKS
                     GNTKDADVQGKQGSQGGGTRETILAAAQKGFASERYRNVYGKYHQIVEDVMRTEKLPS
                     SYKYYVKRYFAKIHPAIEAAAGDASDSPASPGSPASNEDAIP"
BASE COUNT          661 a         1788 c         1908 g          958 t
ORIGIN      
        1 ccccggcggg gcgggcgggg tccaccggac caggctgagc tgctgctcgg ggaacgcgcc
       61 gtcggacacc agcacgatca gcgggttggg ccggtcgcgc agcgcgtcgg ccgcggcgcc
      121 gagcgcgcgg gtcaggtcgg ccggggtgtc gctcgcggcg atgccgtcga cgatcttgtc
      181 gagcatcggc gcgtcgccgg agaaccgcga catcggcgtg gcctggccgt cgaccttcat
      241 gatcatggcg acgtcgccgc cgcccatgcc gtcgatcagg tgcttggccc gcgccttggc
      301 ggcgtcgagc cgcgactggt cgcccttgtc gttgccgtcc atcgccttca tcgaggccga
      361 ggcgtcgaac aggaggacca cgctgcgcgc cttgcggtcg gtcacgccga gcgtcggatc
      421 gagcgcggcg aacagcatga tcgcgatgat gaacaggatc aggagcagcg acagcagccg
      481 cttgagctgc ttccacagcg cgttggcgtc gcgctgctcg agcacgcgct gccacagccg
      541 cgagaacggc acctcgaacc ggcgccgccg catcttgatg atgtaggcgc cgatcgcgag
      601 caccgccgcg ccgccggcga tccacgcgaa caccgcgacc gggatcggcc ccaggatgct
      661 cattgatcgc tccggggctg caccccgccc caccggcaaa gccggtaggg ccccaccccg
      721 cgccgattcg cttcgctcat cttaggaacc ctcccgaccg gaagatccgc agcacgagct
      781 cgtcgaacgg gatgctggtg tgggtccgga agaacgccat cgcgtacttg gtgcagtacg
      841 cctcgagctc cttgcagtag cgctcgtgct cgcgctggta ggcctcgagc agcgacttcg
      901 agaccgtcac ctcgcgggtg tcgccggtct cgcagtcgac cagcgcgagg tcgccgtgga
      961 gctggggctc ggcctcgcgc ttgtcgtaga cctggagcac gaacggctcg aacttgttgt
     1021 agcgcagggt gttgaggccc tgctcgaagc cgaccgggtc gtagaagtcc gagatcagca
     1081 cggcgagccc gcggcgcttg ttctggccga cgaacgtctt catgcactcg gcgaggtcgg
     1141 tctggccgcc gatgtcgcag ttgcgcagga actcgaacac ccggaagatc cggttcttgc
     1201 cgcgcgacgg cggcagccgg tcgatcagct tgccggcgaa cgggatgatc gcgacgcggt
     1261 cgaggttggc caggccgacg taggccagcg cggcgccgac ctgcatcgcg tagtgcagct
     1321 tgggcagcgg cgtgccgatc gccatcgagt cggagacgtc gatcaggatg tagatgtgga
     1381 ggtcctcttc ctcctcgaac agccggagca gcagcttgtc gagccggccg tacaggttcc
     1441 agtcgatgta gcggaagtcg tcgccgcgcg cgtaggtccg gtggtcggcg aactcgatgc
     1501 cggagccgac cttgcgggtc cggcgctcgg cgcgcaggtt cccggcgaac accttgcgcg
     1561 acaccatgtg gaggtgctcg agcgtcttca ggaaccgatc gtcgaacagc gcggtgcgat
     1621 cgcgggcgcg gtggaccggg ccggcggcgg cgggccggtc ggacgggcgg agggcggcgg
     1681 cggagcgggc caagacgact atgcctctcg ggtcgccttg aggatctcgg cgatgatcgc
     1741 gtcggtccgg acgccctcgg cctcgccctc gaagttcatg atcatgcggt ggcgcagcgc
     1801 gggcggcgcg acgtggcgga tgtcgtcgat cgcgaccgcg tagcggccct cgagcagcgc
     1861 gcggatcttg gcggcgagga tgatcgcctg caggccgcgc ggcgacgcgc cgtagcgcat
     1921 gaacttcttc gacatcgccg tggcctcggg gttctccggg tgggtcgcga gcacgacgcg
     1981 caccgcgtag tcctggatct ggcgggcgag cgggaccttg cggaccagct cgcgcatctc
     2041 gaggatgcgg gccttctcga tcaccgcctg cggcgacggc aggtggtcgg tggtcgtccg
     2101 gtccaggatg gtgtggaggt cctccttgcc ggggtactcg acgaccagct tgaagaagaa
     2161 ccgatcgagc tgggcctcgg gcaacggata ggtgccctcc atctcgagcg ggttctgggt
     2221 cgcgagcacg aagaacggct tctcgagctg gtaggtctgc ttggcgacgg tgaccgagcc
     2281 ctcgctcatc gcctcgagca gcgccgactg ggtcttgggc gtcgcccggt tgatctcgtc
     2341 ggcgagcagg atgttggcga agatcgggcc cttctggaac tcgaagcgct tgatgccgtg
     2401 atcctcggtg atcacgttgg tgccgacgat gtcggccggc atcagatcgg gcgtgaactg
     2461 gatccgcgag aacgtggcgt ggatggtctc ggccagcgtg cgcaccagca tggtcttgcc
     2521 caggccgggc acgccctcga gcagcgcgtg gccgccgccg agcaggcaca tgaggacgcc
     2581 ctcgatgatg ccctcctggc cgacgatcat cgtcgagatc tcggcgcgca gccgttgcag
     2641 gtcggtggtg aagctcgcga cggtggtctc gacgtcacgc gcggcggcgg cttgggtcat
     2701 ggcctgggtc atggaatggc gtcctcgttc gatgccggag atcccggcga tgcgggcgaa
     2761 tcgctggcgt cgccggcggc ggcctcgatg gccggatgga tcttcgcgaa gtagcgcttg
     2821 acgtagtact tgtagctcga cggcagcttc tcggtgcgca tcacgtcctc gacgatctgg
     2881 tggtacttgc cgtagacgtt gcggtagcgc tccgacgcga agcccttctg ggccgcggcg
     2941 aggatggtct cgcgggtccc gccgccctgg ctgccctgct tgccctgcac gtcggcgtcc
     3001 ttggtgttgc cggacttctg ggtcggatcg ccgaccaggt tgtcgtcgtg gccgacgccc
     3061 cattcgttgc tggcctgcgg ctggccctgg ccctggccgc cctggcccgg ctggccctgg
     3121 cccggctgct ggccctggcc gggcttccac gcctggctct ggccgcgctg gccgcgcgcg
     3181 cgctgcgcga agtcctggtt cttgttctgc ttgccgaacg gatccttgcc gtccttgccg
     3241 cccttctgct tggcgcgccg catcgcctcg cgcaggtcct ccatctgcga cgacatcttc
     3301 ttctgggtcg cctgcttgcg gcggtcctgg tcgacccggc cggtctcgcg cgccgcgtcc
     3361 ttgagcttct gcgatgcctg ctgcttctgg ccctgctggt cctgctgctc ctggttctgc
     3421 ttgtccttct ggccgtcctt cttctgcatg tcctcggcgg ccttctcgag atcgcgcgac
     3481 agccgcttga gcgcctggcg ctgctcggac tgctccttgt cctcggcgtc cttgtccagc
     3541 ttcttgagct cgtccttgag cttggcgagc tggcgctcgg cgtcgagccg ttcctggtcg
     3601 gtcttggcct cctgcttctt cttctcgagc cggcggatgt cgttctcgag cttctgctgc
     3661 ttctgctgct tctgctggtt cagcttttgc tgttgctgct gcttgtcgtc gagctgcttg
     3721 gccgccttct cgagctgctt ggcgagctcc tgctgctgct gctcggacag ctcgttcttg
     3781 tcgagcttct cggcgagctt ctcgagctcc tgctgggcct tgtcgagctc ggtcttctgc
     3841 agggcgtcgc cgagcgactt ggtcagctgg tccttggaca gctgcttgcc gagctcggcg
     3901 agctgcttgt cgacgtcggc ctggtcgggc tcggcgtgct gctcgagctt ggtctcggcc
     3961 ttggcgagcg cctcgagcag cttctccttg gagatcttgc cgagctcggc gtcctgcagc
     4021 agctgctcga cctgcttggc gaagtcctcg agctcgggga cctgatcgcg cttggcgacc
     4081 gccttgatct cgttcagcag cgcgtcgaga taggcgcggt cgtcgggatc gagcacgacc
     4141 gagtccgcct tgtggtagcg cgggtccttc ttgtcgatca cggtgaacgc cagcggtccg
     4201 accgcgcggt cgccgatcca ggcggtcagc gtggtcggac cgatcggcgc gcggtcgggg
     4261 atctggacca ggatccggtc ggccgcccag tcgagcacgc gggcggggtg gtccttgccg
     4321 gcgccgagcg tgacctggcc atcgctcggg atgaagccgc ggatcatcgg cgcggtctgc
     4381 ggggccgtca ccggggccac cggagccgtc gcctcggcgg ggaccccggg cgcgccgagc
     4441 gacgcgtcga gccacgcgcg cggcggcagg ctcgcgagcg ggtgcgcgat gccgttcatc
     4501 aggttgacgc cgatcagcac cacctgatcc ccggccgcgg cgtgatcggg ctcgacccgg
     4561 tagagatgtg gcgcgcgatc caccgtcggg atcgccaggc cggcggcgag cgccgagatc
     4621 gcgaggaagc cgagcgcggc gcgcaggtcg gtcggtgcgg tgaacggcgt cgcgcgcgcg
     4681 acatcggcgc gcggcacagc gcgcacaccg tcgcggatcg ccgcgtgcat cagctggtgg
     4741 gtctcgtcgt cggcggggag cacgcccgcg cggctgaacg cgaccgccgt cgacagccgg
     4801 tcggacaggt tggacgcccg gtcgatccgc cgcgcgacgt gctcgtcgtc gatccgccgc
     4861 accgccgcga tcaccgcgcc gagcacgatg atcccggcgg tgccggcgag cagcgccagg
     4921 cccgccgcgc gctcgacgat ctcgatccgg atggagaaga cgaccgcgag cgccgccgcc
     4981 gcggccagga tcgtcgccgt ggtggcgccg tccagcgccc gctggccgcg gatccggcgc
     5041 cgggcccgcg cgatcacccg gtggatcggc gatgtgtccg gcgcaacccc gcgctccggt
     5101 tgccctcggt tctcactatc ttgcacggtg ggctccctgc gtgatggacc tccgggggcc
     5161 cagtgatcgt ccaacggttg aggatagcat caacattcca cgtgccaccg gttgagacgg
     5221 tctaacccgg tcccggcccg gcgcctgggc ctgctctgtt gcgacaccgg tgccagccgg
     5281 atggttgccg ggcgggggac ctacacgtag caccc
//
LOCUS       DASVOR010001414         3208 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_365188,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001414 DASVOR010000000
VERSION     DASVOR010001414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3208
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365188"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1233)
                     /gene="hisC"
                     /locus_tag="VF516_26900"
     CDS             complement(115..1233)
                     /gene="hisC"
                     /locus_tag="VF516_26900"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEX8111399.1"
                     /translation="MPTLSPSAFVPPHVASLDVYQPGKPIEELERELGVTGAIKVASN
                     ENPLGPSPRALAALPAALAQLHCYPDAGGFALRRALADRLGCGLGQIALGNGSNDLLY
                     QLVLATCEMTDEVLTHKYAFLSYRLAAQVVGRPFIAAPATPELACDIDALIAAITPRT
                     KLVVIDTPNNPTGSVVTRAGIDRLIAALPERALLVIDEAYAEYAAMWPEVDHADGLVV
                     WRRDPRVIVLRTFSKIYGLAGLRVGFAVADPAVIDVLGRVGRTFHVSSLAMIGALAAL
                     EDTEHVARGAEQARRAIERYTAEVRAPGVRVYPSLGNFVLIDCGRPSTPLYDRLLQRA
                     VIVRPMAAWGLPNHLRVSAARDADMPRVIAALNDVLAT"
     gene            1309..2655
                     /locus_tag="VF516_26905"
     CDS             1309..2655
                     /locus_tag="VF516_26905"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEX8111400.1"
                     /translation="MRRSVVWPAVALALAVLLCPGVAAAAGCDPFSAVQQRGWGPMYI
                     ASFVFGFQTSLTPCVYPMIPITLGIFGARGKGVSRRRALALATAYVVGMGLTYATLGV
                     TIALIGGQFGTLLANPFVVIPIVLLFAALAASMFGAFELNLPASWQARLNQVGGQGYR
                     GAFAMGLVGGLIAAPCTGPFLLGLLTFVATSRSVFGGGSLLFVYALGMGVLFWVLAAF
                     AMSLPKSGRWMEWVKSAGGILLLLGGIYFLKPLLPFMRHLAVPEGWFLAASIAVIAAG
                     LLLGAIHLSFHAPLGEKLRKGLGIALVLAGAFAAWSYKLTPKQKLPYLTDEAAAFARA
                     RAEGKGVMVDFSATWCVPCSELELTFGDDDVYEQITKNFVPLKFDVSDDNETSAERRN
                     RYKAGTLPAVIYLSTDGHALGRVDHMMEPDELAGVIGPAIARLRSGSLLASGEPCR"
     gene            complement(2912..>3208)
                     /locus_tag="VF516_26910"
     CDS             complement(2912..>3208)
                     /locus_tag="VF516_26910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111401.1"
                     /translation="KDAGPRLERVEAFAKDAGPRLERVEAFAKDAAPRLERVEAFAKD
                     AGPRLERVEAFATEAAPRLERIETHLALPAPRPRRETPRPTPSKHHRRKPMKRN"
BASE COUNT          401 a         1196 c         1099 g          512 t
ORIGIN      
        1 cgccgcacgc gatcgcggac gccagcgcga ggagggccgc gcgcaccgtc atcactcgag
       61 gaggaagccg tcgggccctg cggcagcgcg atcgatgccg gaccgacgcc gcggtcatgt
      121 cgccagcacg tcgttgagcg ccgcgatcac gcgcggcatg tcggcgtcgc gcgcggccga
      181 cacccggagg tggttgggca ggccccacgc cgccatgggc cgcacgatca cggcgcgctg
      241 gagcagccgg tcgtagagcg gcgtcgacgg ccggccgcag tcgatcagca cgaaattgcc
      301 gagcgacggg tagacccgca cgcccggcgc gcggacctcg gcggtgtagc gctcgatcgc
      361 ccggcgagcc tgctcggcgc cgcgcgcgac gtgctcggtg tcctcgagcg ccgccagcgc
      421 gccgatcatc gccaggctcg acacgtggaa cgtccgtccg acccggccga gcacgtcgat
      481 caccgccggg tcggcgaccg cgaacccgac gcgcagcccg gcgagcccgt agatcttcga
      541 gaacgtgcgc aggacgatga cgcgcggatc gcggcgccac acgaccagcc cgtcggcgtg
      601 gtcgacctcc ggccacatcg ccgcgtactc ggcgtaggcc tcgtcgatca ccagcagcgc
      661 gcgctcgggc agcgccgcga tcagccggtc gatcccggcg cgggtcacca ccgagccggt
      721 cgggttgttc ggcgtgtcga tcacgacgag cttggtgcgc ggggtgatcg ccgcgatcag
      781 cgcgtcgatg tcgcacgcca gctcgggcgt cgcgggcgcc gcgatgaacg gcctgccgac
      841 cacctgcgcc gccagccggt acgacaggaa cgcgtacttg tgggtcagca cctcgtcggt
      901 catctcgcag gtcgcgagca ccagctggta caagaggtcg ttggacccgt tgccgagcgc
      961 gatctggccc agcccgcagc cgagccggtc cgcgagcgcc cggcgcagcg cgaacccgcc
     1021 ggcgtcgggg tagcagtgga gctgggcgag cgccgccggg agcgccgcga gcgcccgcgg
     1081 cgacggcccc agcgggttct cgttcgacgc gaccttgatc gccccggtga ccccgagctc
     1141 gcgctccagc tcctcgatcg gcttgccggg ctggtacacg tcgaggctcg cgacgtgagg
     1201 cggaacgaag gcgctcggcg agagggtggg cacaacctgg ggaccctaca acgccctcgc
     1261 cccgcgggga atgcccgtgc cgcgcggtcc ggtctatagt agcaactcat gcgccgctcg
     1321 gtcgtctggc ccgccgtggc cctcgcgctc gccgtcctcc tgtgccccgg cgtcgcagcc
     1381 gccgcggggt gcgatccgtt cagcgccgtc cagcaacgcg gctggggccc gatgtacatc
     1441 gcgtcgttcg tgttcgggtt ccagacctcg ctcacgccgt gcgtctaccc gatgatcccg
     1501 atcacgctcg gcatcttcgg cgcgcgcggc aagggcgtca gccgccgccg ggcgctggcg
     1561 ctcgccaccg cgtacgtcgt gggcatgggc ctcacgtacg cgacgctcgg cgtgaccatc
     1621 gcgctgatcg gcgggcagtt cggcaccctc ctcgccaacc cgttcgtcgt gatcccgatc
     1681 gtcctgctgt tcgccgcgct cgcggcctcg atgttcgggg cgttcgagct caacctgccg
     1741 gcgtcgtggc aggcccggct caaccaggtc ggcggccagg gctaccgcgg cgcgttcgcg
     1801 atgggcctgg tcggcggcct gatcgccgcg ccgtgcaccg gcccgttcct gctcggcctg
     1861 ctcacgtttg tcgcgacctc gcgcagcgtg ttcggcggcg gctcgctgct gttcgtctac
     1921 gcgctcggca tgggcgtgct gttctgggtg ctcgcggcgt tcgcgatgtc gctgcccaag
     1981 agcggccggt ggatggagtg ggtgaagtcg gccggcggca tcctgctcct gctcggcggg
     2041 atctactttc tcaagccgct gctgccgttc atgcgccacc tcgccgtccc cgagggctgg
     2101 ttcctcgccg cgtcgatcgc cgtgatcgcc gccggcctcc tgctcggcgc gatccacctg
     2161 tcgttccacg ccccgctcgg cgagaagctg cgcaagggcc tcggcatcgc gctggtgctc
     2221 gccggcgcgt tcgcggcgtg gagctacaag ctcaccccca agcagaagct gccgtacctg
     2281 accgacgagg ccgccgcgtt cgcccgggcg cgcgccgagg gcaagggcgt gatggtcgac
     2341 ttctcggcga cctggtgcgt gccgtgcagc gagctcgagc tgacgttcgg cgacgacgac
     2401 gtctacgagc agatcaccaa gaacttcgtg ccgctcaagt tcgacgtgtc cgacgataac
     2461 gagaccagcg ccgagcgccg caaccgctac aaggcgggca cgctgcccgc ggtgatctac
     2521 ctctcgaccg acggccacgc gctcggccgg gtcgatcaca tgatggagcc cgacgagctc
     2581 gccggcgtga tcggcccggc gatcgcgcgg ctgcgctcgg gctcgctgct cgcctcgggc
     2641 gagccctgcc gctgaccgga tgggaacagt cgcttcagct tcgcgttcgt cacagccaca
     2701 gcttcccaga tgcgctcgct ctcctgctct tcctggcggt ggacccagcg ctggatcgtc
     2761 cggagcatcg cctcctgatg ggccactgtc agcctcacgc tgaacttgac atcgttcacg
     2821 gcgagcctga tctcgtcgag gatcttgatc acctgactgg tcgatggctc cggcgtcgtc
     2881 ctggtcttcg tgggcatcca atctccttcg ttcagttccg cttcatgggt tttctgcggt
     2941 gatgctttga tggtgtgggc ctcggcgtct cccgccgcgg ccggggcgca ggcagcgcga
     3001 ggtgtgtctc gatccgctcc agccgcggcg ccgcctccgt cgcgaacgct tccacccgct
     3061 ccagccgcgg tcccgcgtcc ttcgcgaacg cttccacccg ctccagccgc ggcgccgcgt
     3121 ccttcgcgaa cgcttccacc cgctccagcc gcggtcccgc gtccttcgcg aacgcttcca
     3181 cccgctccag ccgcggtccc gcgtcctt
//
LOCUS       DASVOR010001415         4488 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_219143,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001415 DASVOR010000000
VERSION     DASVOR010001415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4488
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219143"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1862
                     /locus_tag="VF516_26915"
     CDS             <1..1862
                     /locus_tag="VF516_26915"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:2001070 - starch binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate-binding module family 20
                     domain-containing protein"
                     /protein_id="HEX8111402.1"
                     /translation="VAAAGCAAGPSLPGHGPPDVIALDAPRTAFVQLFEWKWTDIARE
                     CETYLGPKGFAAVQISPPNEHNWIPTGDGAPFPWWMRYEPVSYSLDASRSGNRAQFID
                     MVSRCNAAGVGIYADAVINHMSFGEGTVSSAGNHPWSVESFPGVPYGPDNFHPMCTVD
                     NYQDARNVQNCQLLGLPDLDTGSSYVRGKITDYLVDLVNLGVRGFRVDAAKHMSPADV
                     TAIVGAVNSRAATRPYWFLEVIGAAGEAVQPGQYFGVSDGQANVTEFGFGLELFGKFA
                     GGGKLADLRTFGEPWGLLPSNKAVVFLDNHDTQRGHAGDGRVLTYHDGPTYDNAAVFM
                     LAWPYGYPVIMSSFAFNTTSAFDTSHGPPFDPATGATRGPWDGGVSQPACFDQTVGGW
                     VCEHRFRPIGNMVSFRNATVGNWFVSDWWDNGNNQIAFGRGNLGFVVINQESRVLTRS
                     FHTGLPAGDYCDVISGEFTPASGSTPASCTGTVVTVDASGNAAITAPAFGAAAIHAGA
                     TVRGGTTAPTTVTVTFNESADTFLGQNIYMTGSIAELASWNTDGAMPLTRISGSGTLG
                     NWRVTLTLPAATGVEYKYVKKDGAGTVTWESGDNRALTTGAGGSAQTVNDSWK"
     gene            2066..4399
                     /locus_tag="VF516_26920"
     CDS             2066..4399
                     /locus_tag="VF516_26920"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111403.1"
                     /translation="MENNAQAVSALLAALLILAIGTSVLLRSRSDRMYTSFAAFSFTV
                     SAWHLCTSISVATLSPVMHWLALWAAATIPPTAIRFFRIFLAQPSIGGPKRGPRVTLA
                     WTLLAYAALVVSAIYKPGTRPIHDRLYFQVPFVVYVFGGLYRCVLDLYIQYRATAKRV
                     ERTRVGYLALGGFVAITLTLTGTLRLGVTWLAVGNVLGILYLYFLSQTLFRYRLIDLN
                     ELVGKMAVLGSLVVLLWAVNSFLLYWVGNGSAGNPSTSGKDFYLLNALVASFVILILF
                     EPVRSWLETGINRWLLRQRTELRGRLDMVRRELPGVVDVADMVQRITTALEESRRVTD
                     ASVYLLDSDGAGFDRAGCIGRAPPERLDASAERLLLDRVRGGYLDKDQLAHELEDLAA
                     TTQPEAKRVPVLALQTRLDELHAGVIFPLLGSAETEQGPWLLGLFCVRDDRTESAFDP
                     DDLDTFRQLAAGAARVIESSQAYERVKERDRLAALGEMAAGLAHEIRNPLGAIKGAAQ
                     LLITDGVASEAAPAVSSTTEMVELLDIIVEEANRLNNVVTRFLDYARAERPGREGAGK
                     VDLNQVVRKTVQLLQQELPKSIEIRSRIDEMLPPIAGDPESLMQVFLNLGQNALHAMP
                     DGGTLEILTTRRRRSRLGYGQFAEVRFRDTGIGIPRDKLKKLFIPFYTTKQKGTGLGL
                     AISHRIVNQHGGTIEVRSTLGQGSTFSVFLPAAEPVATSEVSDITETGGLRSIKPAER
                     SDAPPPAAAEPSAVATVGKQVESIPPEESESTPTPTV"
BASE COUNT          685 a         1657 c         1506 g          640 t
ORIGIN      
        1 tcgtcgccgc cgccggctgc gcggccggtc cctcgctccc cggccatgga ccgcccgacg
       61 tgatcgcgct cgacgccccg cgcaccgcgt tcgtccagct gttcgagtgg aagtggaccg
      121 acatcgcccg cgagtgcgag acctacctgg gccccaaggg cttcgccgcg gtccagatct
      181 cgccgcccaa cgagcacaac tggatcccca ccggcgacgg tgcgccgttc ccgtggtgga
      241 tgcgctacga gccggtcagc tacagcctcg acgcgagccg cagcggcaac cgcgcccagt
      301 tcatcgacat ggtgagccgc tgcaacgccg ccggggtcgg gatctacgcc gacgcggtga
      361 tcaaccacat gagcttcggg gagggcacgg tgtcgagcgc cggcaaccac ccgtggagcg
      421 tcgagagctt tccgggcgtc ccgtacggcc cggacaactt ccaccccatg tgcacggtcg
      481 acaactacca ggacgcccgc aacgtccaga actgccagct gctcggcctg ccggatctcg
      541 acaccggctc gagctacgtg cgtggcaaga tcaccgacta cctggtcgac ctcgtcaacc
      601 tcggcgtccg cggcttccgc gtcgacgccg ccaagcacat gagcccggcc gacgtcaccg
      661 cgatcgtcgg cgccgtcaac tcccgggccg cgacccggcc gtactggttc ctcgaggtga
      721 tcggcgccgc cggcgaggcc gtgcagcccg gccagtactt cggggtcagc gacggccagg
      781 ccaacgtgac cgagttcggg ttcggcctcg agctgttcgg caagttcgcc ggcggcggca
      841 agctcgccga tctgcgcacc ttcggcgagc cctggggcct cctgccgagc aacaaggcgg
      901 tcgtgttcct cgacaaccac gacacccagc gcggccacgc cggcgacggc cgcgtgctca
      961 cgtaccacga cggcccgacc tacgacaacg ccgccgtgtt catgctggcg tggccgtacg
     1021 gctacccggt gatcatgtcg agcttcgcgt tcaacaccac gagcgcgttc gacaccagcc
     1081 acggcccgcc gttcgatccg gccaccggcg cgacccgcgg cccgtgggac ggcggcgtca
     1141 gccagccggc gtgcttcgac cagacggtcg gcggctgggt ctgcgagcac cgcttccggc
     1201 cgatcggcaa catggtgtcg ttccgcaacg cgaccgtcgg caactggttc gtctcggact
     1261 ggtgggacaa cggcaacaac cagatcgcgt tcggccgcgg caacctcggc ttcgtggtca
     1321 tcaaccagga gagccgtgtg ctgacccgca gcttccacac cggcctgcct gccggtgact
     1381 actgcgacgt catctccggc gagttcacgc cggcgtccgg cagcaccccc gcgagctgca
     1441 ccggcacggt ggtcacggtc gacgcatcgg gcaacgccgc catcaccgcg ccggcgttcg
     1501 gcgccgcggc gatccacgcg ggcgccacgg tccgcggcgg cacgaccgcg cccaccacgg
     1561 tcaccgtcac gttcaacgag tccgccgaca cgttcctcgg ccagaacatc tacatgaccg
     1621 gcagcatcgc cgagctcgcg agctggaaca cggacggcgc gatgccgctg acccggatct
     1681 cgggcagcgg cacgctcggc aactggcggg tcaccctgac cctgccggcg gcgaccggcg
     1741 tcgagtacaa gtacgtcaag aaggacggcg ccggcaccgt gacctgggag tccggcgaca
     1801 accgcgcgct caccaccggg gccggcggca gcgcgcagac cgtcaacgac agctggaaat
     1861 gaccacaccc ggctgggggc gctcgccggg gaaggccgtg gtgaagcgga agaaccgccc
     1921 agcctgacga agacctgagc gatctcgctc tgtttgaact atccggaacg tccggacggt
     1981 tgcctcgagc tcgagatatc ctccccgtag ctggcagccc gccatcgccg cgaactgcgc
     2041 cggcgccatc cggtagactc gtcacgtgga gaacaacgcc caggcggtat ccgccctgct
     2101 cgcggcgctg ctgatcctcg cgatcggcac gtcggtgctg ctgcggtcgc gctcggaccg
     2161 gatgtacacg tcgttcgccg cgttctcgtt cacggtctcg gcctggcacc tgtgcacgtc
     2221 gatcagcgtc gcgaccttga gcccggtgat gcactggctg gcgctgtggg ccgccgcgac
     2281 gatcccgccg accgcgatcc ggttcttccg gatctttttg gcccagccgt cgatcggcgg
     2341 gcccaagcgc gggccgcggg tcaccctggc gtggacgctg ctggcctacg cggcgctcgt
     2401 ggtgtcggcg atctacaagc cgggcacccg gccgatccac gaccggctgt acttccaggt
     2461 cccgttcgtc gtctacgtgt tcggcgggct gtaccgctgc gtgctcgacc tctacatcca
     2521 gtaccgggcg accgccaagc gggtcgagcg cacgcgggtc ggctacctcg cgctcggcgg
     2581 gttcgtcgcg atcacgctga ccctgaccgg cacgctgcgg ctcggcgtca cctggctcgc
     2641 ggtcggcaac gtgctcggca tcctgtacct gtacttcctg tcgcagacgc tgtttcgcta
     2701 ccggctgatc gacctcaacg agctggtcgg caagatggcg gtgctcggct cgctggtcgt
     2761 gctgctgtgg gcggtcaaca gcttcctctt gtactgggtc ggcaacggca gcgcgggcaa
     2821 cccgagcacg agcggcaagg acttctacct gctcaacgcg ctggtcgcgt cgttcgtgat
     2881 cctgatcctg ttcgagccgg tgcgcagctg gctggagacc gggatcaacc gctggctgct
     2941 gcgccagcgc accgagctgc gcggccggct cgacatggtc cggcgcgagc tgccgggcgt
     3001 ggtcgacgtc gccgacatgg tgcagcggat caccaccgcg ctcgaggagt cgcgccgggt
     3061 caccgacgca tcggtctacc tgctcgactc cgacggcgcc gggttcgacc gcgcggggtg
     3121 catcggccgg gccccgcccg agcggctcga cgccagcgcc gagcggctgc tgctcgaccg
     3181 cgtgcgcggc ggctacctcg acaaggacca gctcgcccac gagctcgagg acctcgccgc
     3241 gaccacccag cccgaggcca agcgggtgcc ggtgctcgcg ctgcagaccc ggctcgacga
     3301 gctccacgcc ggcgtgatct tcccgctgct cggctcggcc gagaccgagc aggggccgtg
     3361 gctgctcggc ctgttctgcg tgcgcgacga tcgcaccgag agcgcgttcg accccgacga
     3421 cctcgacacg ttccgccagc tcgcggccgg cgcagcccgc gtgatcgagt cgagccaggc
     3481 ctacgagcgg gtcaaggagc gcgaccggct cgcggcgctc ggcgagatgg cggccggcct
     3541 cgcccacgag atccgcaacc cgctcggcgc gatcaagggc gcggcccagc tgctgatcac
     3601 cgacggcgtg gcgagcgagg ccgcgccggc ggtgagctcg acgaccgaga tggtcgagct
     3661 gctcgacatc atcgtcgagg aggccaaccg gctcaacaac gtcgtgaccc ggttcctcga
     3721 ctacgcgcgc gccgagcgcc ccggccgcga gggcgccggc aaggtcgacc tcaaccaggt
     3781 ggtgcgcaag accgtgcagc tgctccagca ggagctgccg aagtcgatcg agatccggag
     3841 ccggatcgac gagatgctgc cgccgatcgc cggcgacccc gagtcgctga tgcaggtgtt
     3901 cttgaacctc gggcagaacg cgctgcacgc gatgcccgac ggcggcacgc tggagatcct
     3961 gaccacgcgg cgccgccgat cgcggctcgg ctacggccag ttcgccgagg tccggttccg
     4021 cgacaccggc atcggcatcc cgcgcgacaa gctcaagaag ctgttcatcc cgttctacac
     4081 gaccaagcag aagggcaccg gcctgggcct ggcgatctcg caccgcatcg tcaaccagca
     4141 cggcggcacg atcgaggtcc gctcgacgct cggccagggc tcgacgttct cggtgttcct
     4201 gccggccgcc gagccggtcg cgaccagcga ggtcagcgac atcaccgaga ccggcgggct
     4261 gcgctcgatc aagccggccg agcgcagcga tgcaccgccg cccgccgccg ccgagcccag
     4321 cgcggtcgcg acggtcggca agcaggtcga gtcgattccg cccgaagaga gcgagtcgac
     4381 cccgacgccc acggtataag gagggaccgt gcagccggcg aagatcctcg ttgccgacga
     4441 cgagcagaac ctgcgacgcg tcctcgtcgc gctgctgcgc cgcgaggg
//
LOCUS       DASVOR010001416         2518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_15297,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001416 DASVOR010000000
VERSION     DASVOR010001416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15297"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..331
                     /locus_tag="VF516_26925"
     CDS             <1..331
                     /locus_tag="VF516_26925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111404.1"
                     /translation="ADAQRRSAMTAGFRATGCGVIEAATPLEAIVRLGESSFEPDVIA
                     IADSHPAAAADEMRGFVERNHPNAKLVTIGDALLQPDGIAHWLSSADPASDLTARIRE
                     VLVRPRP"
     gene            complement(415..693)
                     /locus_tag="VF516_26930"
     CDS             complement(415..693)
                     /locus_tag="VF516_26930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111405.1"
                     /translation="MARTFVRVELEHAHAERSFSIAMGELGFLRTVKGRKTRKTLRLP
                     SGMYVIRRTSPVEALELVRQAARDSKVRARIFCVPVDGNIRFSNLRPI"
     gene            759..1037
                     /locus_tag="VF516_26935"
     CDS             759..1037
                     /locus_tag="VF516_26935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111406.1"
                     /translation="MTNRLYVGNLASHAESELRPLATAIGESWAAELVHSLRSDHRDV
                     VGEWPGTLGEARMRIRVAFRRHIDARLLDELARVANLAARRGWLEVRD"
     gene            1351..2253
                     /gene="nhaR"
                     /locus_tag="VF516_26940"
     CDS             1351..2253
                     /gene="nhaR"
                     /locus_tag="VF516_26940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HEX8111407.1"
                     /translation="MEWLNYHHLLYFWMVAKHGSITQASKELRLAHPTISGQIHRLEA
                     VLGEKLFARSGRNLVLTESGRIAFRYADEIFALGQEFQDTLKGRSTGQQARLVVGVSD
                     VLAKSIVHRILEPAFQLQDRVRIICREARSADAFMGELAVHAIDVVLADAPAGPGTPV
                     RTFSHPLGDCGSSFFAAPKLARAHRRGFPGSLDGVPLLLPSGDSTFRRVLDEWFQSHD
                     IRPEIVAELDDLALASVLGEEGLGVFAAPDVIAKELRQRYALELVGRAKDIRQRFYAI
                     SVERKIKNPAVVAICEAARKNILA"
     gene            complement(2257..2412)
                     /locus_tag="VF516_26945"
     CDS             complement(2257..2412)
                     /locus_tag="VF516_26945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111408.1"
                     /translation="MKRCELEVRIKPQVMKVEHSDTDVLLALEHAARRAARSVSPTIE
                     TASLVRR"
BASE COUNT          413 a          863 c          839 g          403 t
ORIGIN      
        1 cgccgatgcg cagcgccggt cggcgatgac cgcgggattt cgcgccaccg gctgcggggt
       61 catcgaggcg gcgaccccgc tcgaagcgat cgtgcggctg ggcgagtcga gcttcgaacc
      121 cgatgtgatc gcgatcgccg actcccatcc ggcggcggcg gccgacgaga tgcgcggctt
      181 cgtcgagcgc aaccacccga acgcgaagct ggtcacgatc ggagatgcgc tgctgcagcc
      241 cgacgggatc gcgcactggc tgtcgtcggc cgatccggcc agcgacctca ccgctcgcat
      301 ccgcgaggtc ctcgtacggc ctcgtccctg atctgccacg ccaccccgct ggcgcgagcg
      361 cgcggggacg agacgcgtcc atccgctgga atgatatttg gaatacgaac tacatcagat
      421 gggccgcaga ttcgaaaacc ggatgttgcc atcgaccggc acgcagaaga tgcgcgctcg
      481 gaccttgctg tcgcgcgctg cctgtcgcac gagctcgagc gcctcgaccg ggctggttcg
      541 cctgatcacg tacatgccgc tcggcagacg aagtgtcttc cgcgtcttcc tgcccttgac
      601 agtacgcagg aatccaagct cgcccatcgc gatcgagaac gagcgttcgg catgggcatg
      661 ctcgagctcg acgcgtacaa acgtcctggc catcgcgaca gtgtagcgca tcgccgacga
      721 aaaccgaccg attttgcaga tggtgagagg aacgacatat gacgaatcgc ctgtacgtag
      781 gtaatcttgc ctctcacgct gaatccgagc tccggccgct ggcgacggcg atcggcgagt
      841 cgtgggccgc cgagctcgtg cactcgctgc gctccgacca tcgcgacgtc gtcggcgagt
      901 ggccgggcac cctgggcgag gcccgcatgc gaatccgcgt cgcgttccgg aggcacatcg
      961 acgctcgact cctggacgag ctcgcgcgcg tcgcgaacct cgcggcgcgc cgcggctggc
     1021 tcgaggtccg ggactgaccc aggatcgcgg ccggtcgagc gtgtcgcaag ggcgaactcg
     1081 ccaagctgcg gtggggagct ggtcgacctc gcgcgcaacg tgatcacccc gagcggcggc
     1141 agtcctcgac ctgccggcga cgtgcctgat ccagaggtca gcgccgtgca ccgatcatcg
     1201 agtgcacggg cgcctcgcga ggttgcttcc cggcgatcgc gtaccgccag agacgcgatc
     1261 ggtcaccaag gcgtccagcg cggcccgttc tgccgagaaa ttcgacgtga atgccggtat
     1321 caccgaagaa ctcgacatac tctgtgctga atggaatggc tcaattacca tcacctgctg
     1381 tacttctgga tggtcgcgaa gcacgggagc atcacgcagg cgagcaagga gcttcgcctg
     1441 gcgcacccga cgatcagtgg ccagatccat cggctcgagg cggtgctcgg tgagaagctg
     1501 tttgcccgca gcgggcgcaa cctcgtcctg accgagagtg gacggatcgc cttccgctat
     1561 gcggacgaga tcttcgcgct cggccaggag ttccaggaca cgctcaaggg ccggtcgacc
     1621 ggccagcagg cgcggctcgt ggtcggcgtg tccgacgtgc tcgcaaagtc gatcgtgcac
     1681 cgcatcctcg agccggcgtt ccagctccag gacagggtcc gcatcatctg ccgggaggcg
     1741 cgctcggccg acgcgttcat gggcgagctc gcggtgcacg cgatcgacgt cgtgctcgcc
     1801 gatgcaccgg cggggccggg cacgccggtc cgcacgttca gccacccgct cggcgactgc
     1861 ggctcgtcgt tcttcgccgc acccaagctg gcccgggcgc accgccgggg attcccgggc
     1921 tcactcgacg gcgttcccct gctacttccg agcggcgact cgacgtttcg ccgcgtgctc
     1981 gacgaatggt tccagtccca cgatattcgc cccgagatcg tcgccgagct cgatgacctc
     2041 gcgctcgcgt cggtgctggg cgaggagggg ctcggcgtgt tcgccgcacc ggacgtgatc
     2101 gcgaaggagc tccgccagcg ctacgcgctg gagctcgtcg gccgcgcgaa ggacatccgc
     2161 cagcggttct acgcgatctc cgtcgagcgc aagatcaaga accccgcggt cgtggcgatc
     2221 tgcgaggccg cgcgcaagaa catcctggcc tgagcctcag cgacggacca gcgacgcggt
     2281 ctcgatcgtg gggctcaccg agcgcgcggc gcggcgcgcc gcatgttcga gggcgagcaa
     2341 cacgtcggtg tcggagtgct cgaccttcat gacctgcggc ttgatccgca cttcgagctc
     2401 gcagcgcttc aagccgggct tctcgcgcgt ccgacaacct gacgatcacg cgtcgatccg
     2461 ttcgccgaag cgcaccagca cggagtcaac caggcgctgc aatcgacggg gttgcgct
//
LOCUS       DASVOR010001417         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_393795,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001417 DASVOR010000000
VERSION     DASVOR010001417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393795"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="VF516_26950"
     CDS             complement(<1..1243)
                     /locus_tag="VF516_26950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8111409.1"
                     /translation="MPHASEAQPYPVIATRVDARSDEFRANAEANRAAVARLSAALHE
                     ATLGGGEKYVARHRAAGKLLPRERVELVLDRDSYFLELCPLAGKDVPGHTPGAGLIGG
                     IGRVSGVECLITASESTVKGGAVSEMTAWKSGRMSDIAEHNRLPSISMIESAGADLPV
                     QSKIFVPGGRGFRDLTRRSQSRIPTICLVFGSSTAGGAYIPGMSDYVVMVENQAQVYL
                     AGPPLVKMATGEDTDHEELGGAAMHSRISGVSDYLARDERDAIRIGREIVAHLEWRKA
                     AQPLPRPIEPPRYDPEDLLGIASPDVKVPFDQREVIARIVDGSRFSEFKPLYGTTLVC
                     GWAHVHGYPVGILANNGILFSESSQKGAQFIELCNSSDVPLVFLQNITGFMVGKKYEQ
                     EGIIKHGAKLINAVSNSTVPAI"
     gene            1307..1873
                     /locus_tag="VF516_26955"
     CDS             1307..1873
                     /locus_tag="VF516_26955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111410.1"
                     /translation="MANVIEEAKSGRASCRTCRKPIAKGELRLGVETQTQFSDTPSMQ
                     WHHLPCAAGKHPVELRAALAEYPGEVPSRAELDAALEQADKQAAAKPAGHPYADHAPT
                     GRAKCMECGQPIEKGSFRVAVERELEIGGNATRGTGYLHPGCVAANLENVGGSVDEVI
                     EGLRVNSRLTEAELDGVIADVEQGGDAA"
     gene            complement(1877..>2168)
                     /locus_tag="VF516_26960"
     CDS             complement(1877..>2168)
                     /locus_tag="VF516_26960"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8111411.1"
                     /translation="KLADMASRIAAARALTGEAVTRVLRGEQAAGLAAMAKNVATDMC
                     SFVCDQAVQLHGGHGYMRETLVERLYRDARLYPIGGGTREIMNEVIAKTERY"
BASE COUNT          320 a          782 c          761 g          305 t
ORIGIN      
        1 tgatcgcggg gacggtcgag ttcgacaccg cgttgatcag cttggcgccg tgcttgatga
       61 tgccctcctg ctcgtacttc ttgccgacca tgaagccggt gatgttctgg aggaacacca
      121 gcggcacgtc gctcgagttg cacagctcga tgaactgcgc gcccttctgc gagctctccg
      181 agaacaggat gccattattg gcgaggatgc cgaccgggta gccgtggacg tgggcccagc
      241 cgcacaccag ggtcgtgccg tacagcggct tgaactcgga gaaccgcgag ccgtcgacga
      301 tccgcgcgat cacctcgcgc tgatcgaacg gcaccttgac gtcgggagac gcgatgccga
      361 gcaggtcctc ggggtcgtag cgcggcggct cgatcgggcg cggcagcggc tgggccgcct
      421 tgcgccactc gaggtgggcg acgatctcgc ggccgatccg gatcgcgtcg cgctcgtcgc
      481 gcgcgaggta gtcggacacg cccgagatcc gcgagtgcat cgccgcgccg ccgagctcct
      541 cgtggtcggt gtcctcgccg gtcgccatct tcaccagcgg cgggccggcg aggtagacct
      601 gcgcctggtt ctcgaccatg accacgtagt cgctcatccc ggggatgtag gcgccgcccg
      661 cggtcgagct gccgaacacc aggcagatcg tcgggatccg cgactgcgag cgccgggtca
      721 ggtcgcgaaa cccgcggccg ccgggcacga agatcttcga ctgcaccggc aggtcggcgc
      781 cggccgactc gatcatcgag atcgacggca ggcggttgtg ctcggcgatg tcggacatcc
      841 gccccgactt ccacgcggtc atctcgctga ccgcgccgcc cttgacggtc gactcgctcg
      901 cggtgatcag gcactcgacg cccgacaccc ggccgatccc gccgatcagg ccggcgcccg
      961 gcgtgtggcc cggcacgtcc ttgccggcga gcggacacag ctcgaggaag tacgagtcgc
     1021 gatcgagcac gagctcgacc cgctcgcgcg gcagcagctt gccggccgcc cggtgccggg
     1081 cgacgtactt ctcgccgccg cccagcgtcg cctcgtgcag cgcggccgac agccgggcca
     1141 ccgcggcgcg gttggcctcg gcgttggcgc gaaactcatc ggaccgcgcg tcgacgcgcg
     1201 tcgcgatcac ggggtacggc tgggcttcgg aggcgtgtgg cacgcgcgca cgttacccga
     1261 tccggcgggc gacgccctcc ggatcgcggt gtaggctgtg cctgccatgg ccaacgtgat
     1321 cgaagaagcc aagtcgggcc gcgccagctg ccgcacctgc cgcaagccga tcgccaaggg
     1381 cgagctgcgg ctcggcgtcg agacccagac ccagttcagc gacacgccga gcatgcagtg
     1441 gcaccacctt ccgtgcgccg ccggcaagca cccggtggag ctccgcgccg cgctcgccga
     1501 gtaccccggt gaggtgccca gccgcgccga gctcgacgcg gccctggagc aggccgacaa
     1561 gcaggccgcc gccaagcccg ccgggcaccc ctacgccgac cacgccccga ccggccgcgc
     1621 caagtgcatg gagtgcgggc agccgatcga gaagggctcg ttccgcgtcg cggtcgagcg
     1681 cgagctcgag atcggcggca acgcgacccg cggcacgggc tacctgcacc ccggctgcgt
     1741 cgccgccaac ctggagaacg tcggcggatc ggtcgacgag gtgatcgagg gcctgcgcgt
     1801 caactcgcgg ctgaccgagg ccgagctcga cggcgtgatc gccgacgtcg agcagggggg
     1861 cgacgcagca tgacgatcaa tatcgctcgg tcttggcgat cacctcgttc atgatctcgc
     1921 gggtcccgcc gccgatcgga tacagccggg cgtcgcggta cagccgctcg accagggtct
     1981 cgcgcatgta gccgtgaccg ccgtgcagct ggaccgcctg atcgcagacg aagctgcaca
     2041 tgtcggtcgc gacgttcttg gccatcgcgg ccaggccggc ggcctgctcg ccgcgcagca
     2101 cgcgggtcac cgcctcgccg gtcagcgcgc gcgccgcggc gatccggctc gccatgtcgg
     2161 cgagcttg
//
LOCUS       DASVOR010001418         3540 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_102900,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001418 DASVOR010000000
VERSION     DASVOR010001418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3540
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102900"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1739)
                     /gene="malZ"
                     /locus_tag="VF516_26965"
     CDS             complement(<1..1739)
                     /gene="malZ"
                     /locus_tag="VF516_26965"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF008051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HEX8111412.1"
                     /translation="MLLLHPPIAPWLVRTAAGCRVALLCRGEPRIAYLRAEPDNEELL
                     IAMQPVGEVNGWTWFEAVLPWDRGNDTTHYAFKVIHDRRQSWLAADGVHPHPPPRELH
                     FKVCRAHTPPAWIADQIVYQIFPDRFCQGDPSIAVATDEYVYGTGRMRVVRRPWGAPI
                     DPATAATAFYGGDLIGIRRQLDYLAGELGVTALYLNPIFTSGSNHKYDTEDYDHVDPH
                     LGGNAALAELAAALRSRGMRLVLDAVVNHTGANHPWFDRFGRHGGGAHGSPDSPHRAW
                     YVFERSETGGGNPDGSAGGAGGGAPRGIDGAGYASWKGHASLPVLDFAEPAVRAAIYG
                     DRDAVLRRWLRPPYAIDGWRFDVVHMLGEGPGAWNNAHHVRQMRRAVRDENPDGYVLG
                     EHFGEATRWLQGDQEDGAMNYYGFAHPVRAWLAGHDLAGEPAELSAGELARWLTAARA
                     RVPYDNQLAQLNLIDSHDTTRLLTAVGGDPARMALAVTLLFTYPGVPCIYYGDEIGLE
                     GGRDPDCRRCFDWDRAHWNADLHAHYRRLIGWRKARPEWRRGAYQTLAAEGDALVFAR
                     YTERSVTLVAVNRG"
     gene            complement(1818..2426)
                     /locus_tag="VF516_26970"
     CDS             complement(1818..2426)
                     /locus_tag="VF516_26970"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8111413.1"
                     /translation="MKRTLVLVRHGETAWNREGRFQGHTDIALSDVGRAQARALRARL
                     EAAHGYLFDDAQTAILTSDLRRAHETAEIAFSAPGRTLHIRPELREFCYGVFEGLTRR
                     EIDERYPGVMAAWLTGDRTFAVDGGESRAQVHARVHAAVRGFLDQAPQRHVVIVAHGG
                     VMRQLLAACFDPDGEPRGLGYGNTATHVIHVDPRAWSYVGQL"
     gene            complement(2613..3440)
                     /locus_tag="VF516_26975"
     CDS             complement(2613..3440)
                     /locus_tag="VF516_26975"
                     /inference="COORDINATES: protein motif:HMM:NF025585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4331 family protein"
                     /protein_id="HEX8111414.1"
                     /translation="MTTRTHLFRGATALALIVTAACGSSSSGPGPDAGPGDGSGPPPT
                     TTTFHQVEQLARPGINEALLISEAFNAGFNATAPSFTGVPADTLNAVVGEAKTVLEAL
                     YLGGCLINGVAGLTPDTGVKPAAIKCHAVGPAIWMADGVTLTPESKAAAAAYADKVFN
                     QFIPDVLRVDTSVTSSYLVPCGTLDSTPLLCGGRFLDDDVIDVTYDYLLNGAANYLTP
                     GDGGALDQQVLALVSDGVSFSKDPARNSLSLSKPDPADQQQGHPDVTPTFPYSAPPF"
BASE COUNT          470 a         1279 c         1296 g          495 t
ORIGIN      
        1 ccgcggttga ccgcgaccag cgtgaccgag cgctcggtgt agcgcgcgaa caccagcgcg
       61 tcgccttcgg ccgccagcgt ctggtaggcg ccgcggcgcc actccggccg cgccttgcgc
      121 cacccgatca gccggcggta gtgcgcgtgc aggtcggcgt tccagtgcgc gcggtcccag
      181 tcgaagcagc gccggcagtc cggatcgcgg ccgccctcca ggccgatctc gtcgccgtag
      241 tagatgcacg gcacgccggg gtaggtgaac agcagggtca ccgccagcgc catgcgcgcc
      301 ggatcgccgc cgaccgcggt cagcagccgc gtcgtgtcgt ggctgtcgat caggttgagc
      361 tgggcgagct ggttgtcgta cggcacccgc gcccgcgccg cggtgagcca gcgcgcgagc
      421 tcgccggcgg acagctcggc cggctcgccc gcgaggtcgt gcccggcgag ccacgcccgc
      481 accgggtgcg cgaagccgta gtagttcatc gcgccgtcct cctggtcgcc ctgcagccag
      541 cgcgtcgcct cgccgaagtg ctcgcccagc acgtagccgt cggggttctc gtcgcgcacc
      601 gcgcgccgca tctgccgcac gtggtgggcg ttgttccacg cccccgggcc ctcgcccagc
      661 atgtggacga cgtcgaaccg ccagccatcg atcgcgtacg gcgggcgcag ccagcgccgc
      721 agcacggcgt cgcgatcgcc gtagatcgcc gcgcgcaccg cgggctcggc gaagtcgagc
      781 accggcagcg aggcgtggcc cttccagctc gcataccccg cgccgtcgat cccacggggg
      841 gctccgcccc ccgccccacc ggcggagccg tcggggttcc caccccccgt ctcgctgcgc
      901 tcgaacacgt accacgcgcg gtgcggcgag tccggcgagc cgtgggcgcc gccgccgtgc
      961 cggccgaacc gatcgaacca cgggtggttg gcgccggtgt ggttgaccac cgcgtcgagc
     1021 accagccgca tgccgcggct gcgcagcgcg gcggcgagct cggccagcgc ggcgttgccg
     1081 ccgaggtgcg ggtcgacgtg gtcgtagtcc tcggtgtcgt acttgtggtt ggagcccgac
     1141 gtgaagatcg ggttcaggta cagcgccgtg acgccgagct cgccggcgag gtagtcgagc
     1201 tggcggcgga tgccgatcag gtcgccgccg tagaacgcgg tcgccgcggt cgccggatcg
     1261 atcggcgcgc cccacggccg gcgcaccacg cgcatccggc cggtgccgta gacgtactcg
     1321 tcggtggcga ccgcgatcga cggatcgccc tggcagaacc gatcggggaa gatctggtag
     1381 acgatctggt ccgcgatcca ggccggtggg gtgtgcgcgc ggcacacctt gaagtgcagc
     1441 tcgcgcggcg gcgggtgcgg gtgcacgcca tcggccgcca gccagctctg ccggcggtcg
     1501 tggatcacct tgaacgcgta gtgggtggtg tcgttgccgc ggtcccacgg cagcacggcc
     1561 tcgaaccagg tccaaccgtt gacctccccg accggctgca tcgcgatcag cagctcctcg
     1621 ttgtccggct cggcgcgcag gtaggcgatg cgcggctcac cgcggcacag cagcgccacc
     1681 cggcagccag ccgcggtgcg gacgagccag ggcgcgatcg gcgggtggag cagcagcacc
     1741 tcggccggcg agcgtcgtct cgaccgccgc gcgaggcaag atcgcacggc ggcaatcgcg
     1801 cggccgcgcg ccggcgctca cagctggccg acgtacgacc acgccctcgg atcgacgtgg
     1861 atgacgtggg tcgcggtgtt gccgtagccc agcccgcggg gctcgccgtc ggggtcgaag
     1921 cacgccgcca gcagctgccg catcacgccg ccgtgcgcca cgatcacgac gtggcgctgc
     1981 ggcgcctggt cgaggaagcc gcggaccgcg gcgtggaccc gggcgtggac ctgggcccgg
     2041 ctctcgccgc cgtcgaccgc gaacgtgcga tcgccggtca gccacgcggc catcaccccg
     2101 gggtagcgct cgtcgatctc gcgccgggta agcccctcga atacaccgta gcagaactcc
     2161 cggagctcgg gccggatgtg cagcgtgcgg cccggcgcgc tgaacgcgat ctcggcggtc
     2221 tcgtgggcgc gccgcaggtc cgaggtcagg atcgcggtct gcgcgtcgtc gaacaggtac
     2281 ccgtgcgcgg cctcgagccg ggcgcgcagc gcgcgggcct gggcgcgccc gacgtcgctg
     2341 agcgcgatgt cggtgtgacc ctggaaccgc ccctcgcggt tccaggcggt ctcaccgtgg
     2401 cgaaccagga ccagcgttcg cttcatcgct ccggcgacat accacgcgcc gggcgagccg
     2461 tgcagtccta ctcgaaggtg tacctgccga agtcgatccg cgcacaacgc gccgtcgacg
     2521 tcgcttgccg tggaataccg gattcccggt gatcgcgacg cttgcgcggc gcaagggatg
     2581 acgatcgccg ggtcctgcca cgcgcggctc gatcagaacg gcggcgccga gtacgggaac
     2641 gtcggcgtga cgtccggatg cccctgctgc tggtcggcgg gatccggctt cgacaggctg
     2701 agcgagttcc tggccgggtc cttcgagaac gacacgccat cgctgaccag cgcgagcacc
     2761 tgctggtcca gcgcgccgcc gtcgcccggc gtcaggtagt tcgcggcgcc gttgagcagg
     2821 tagtcgtacg tcacgtcgat gacgtcatcg tcgaggaacc ggccgccgca caagagcggc
     2881 gtcgagtcca gggtcccgca cggcaccagg tagctgctcg tgacgctggt gtcgacccgc
     2941 agcacgtccg ggatgaactg gttgaacacc ttgtcggcgt aggccgccgc cgcggccttg
     3001 gactcgggcg tcagcgtgac gccgtcggcc atccagatcg ccggcccgac cgcgtggcac
     3061 ttgatcgcgg ccggcttgac gccggtgtcc ggggtcagcc cggcgacgcc gttgatcagg
     3121 cagccgccga ggtacagcgc ctccagcacg gtcttggcct cgccgaccac ggcgttcagc
     3181 gtgtcggccg gcacgccggt gaagctcggc gcggtcgcgt tgaacccggc gttgaacgcc
     3241 tcgctgatca gcagcgcctc gttgatgccg ggccgcgcga gctgctcgac ctggtggaag
     3301 gtggtggtgg tcggcggcgg gcccgagccg tcgcccgggc ccgcgtcggg gccggggccg
     3361 gacgagctgc tgccgcaggc agcggtgacg atcagcgcga gcgcggtcgc gccacggaat
     3421 agatgagtgc gtgtggtcat ggtcaattcc ctttcctcgg tccccgtcac tcggacaccg
     3481 agatcgtcga ccacgtgtcg aagatcgcgc cgccgccgag ctcggcgatc gggacgttca
//
LOCUS       DASVOR010001419         4296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_307000,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001419 DASVOR010000000
VERSION     DASVOR010001419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307000"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..440)
                     /locus_tag="VF516_26980"
     CDS             complement(90..440)
                     /locus_tag="VF516_26980"
                     /inference="COORDINATES: protein motif:HMM:NF014534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HEX8111415.1"
                     /translation="MSFPIEVRFRDMETSPALEQFVRRWAAKLGRVHDRIGSCEVVIE
                     RPHQSQHQGQHVHVRITLALPGPDIVVSHGQERDGAHEDAYVAVRDAFRAARRQLEQH
                     ARRLRSSELEAPRI"
     gene            complement(662..1531)
                     /locus_tag="VF516_26985"
     CDS             complement(662..1531)
                     /locus_tag="VF516_26985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111416.1"
                     /translation="MTQSRWLSILPLSTALLIPACMAAPDDDVGPADDGTAVHLMPMH
                     DPDAKVADAAPAGARLTFFGGPTLTHVSVHPVFWNANVQFQTNLNAFYKAVTKSALYD
                     MLKQYSSIGRGSGVNGFVDNRTATSVSDAAIHTELNRLFAAGSLPPPSASNYYPVHFP
                     AGVTITAPDGSRSCVTFCAYHGTYVRNGVNVNYGVVPDQGGSCAGGCGRNAQRVNNLD
                     SVASHELIEATTDPAVGLATTFGAPLAWYDRTNGEIGDICNGQQGTTVGNDGVTYVIQ
                     LEFSNSARNCVAR"
     gene            complement(2130..2261)
                     /locus_tag="VF516_26990"
     CDS             complement(2130..2261)
                     /locus_tag="VF516_26990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111417.1"
                     /translation="METQAEIAKAASGFRKKTEAIRSSAYSFPRKRDAERSRDLGWR"
     gene            2693..3892
                     /locus_tag="VF516_26995"
     CDS             2693..3892
                     /locus_tag="VF516_26995"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8111418.1"
                     /translation="MDSVSGKIEEGKGLGPTFNGNSCAQCHAEPDVGGSSPHPTLGHA
                     RMPNPQVGLATLDRAAGGAQKVPKFITPDGPVREARFVKNPDGTDDGGVHGLYTIAGR
                     TDAPGCTLAQPNFDKELANNNVIFRIPTAVFGLGLLENVPDDELVANLAASADAAGKS
                     GCGGAGGVAGELNRSGNDGTITRFGWKAQNKSLLVFAGEAYNVEQGVSNELFNNERAA
                     VGGCVFNGSPEDATDTTTGGAADTTMFAAFMRLSQDPQPTTASPSELRGQKLFGTKAD
                     PGVGCVLCHTDTLTTGAVRYTGMSNVTIHPYTDLAIHHMGTKLADGVTQGAATGDMFR
                     TAPLWGVGKRIFFLHDGRSGPASGGLVDAIRQHGSRGSEASVVIRKFTALPSADQQAI
                     IDFLRSL"
     gene            complement(3905..>4296)
                     /locus_tag="VF516_27000"
     CDS             complement(3905..>4296)
                     /locus_tag="VF516_27000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111419.1"
                     /translation="FHATGDATARLASAFHAAVLQGDLPGIARLLAEDAILYTDGGGK
                     RDAALNPIYGRDKILRFLLGLDRQRRLPVPDEVIATTINGLPGFVLRTGAGTEAEIAT
                     IALEVSDGAIVAIYTVRNPDKLRHLTQ"
BASE COUNT          692 a         1437 c         1498 g          669 t
ORIGIN      
        1 ggatcgcgcg cggcacgccg ccgcccgagc ggatcgatcc ggacgactcc gaccggccgc
       61 gcgaccgctg acccgctcgc ccgggtcgat cagatccgcg gcgcctccag ctcggagctg
      121 cgcagccggc gcgcgtgctg ctcgagctgg cgccgcgcgg cgcggaacgc gtcgcggacc
      181 gcgacgtagg cgtcctcgtg cgccccgtcg cgctcctggc cgtggctgac cacgatgtcg
      241 ggccccggga gggcgagcgt gatccggacg tggacatgct ggccctggtg ctggctctgg
      301 tgcggccgtt cgatcaccac ctcgcagctc ccgatccgat cgtgcacgcg gccgagcttg
      361 gcggcccagc ggcggacgaa ctgctccagc gcgggagacg tctccatgtc tcgaaacctg
      421 acctcgatgg ggaacgacat gatccgacgt ggtgcatgga ccgggccgac gtgggcgcgc
      481 acccggcggt gacgcacagc gatcagccgc gggcggatcg cgcaccactt gcgggggccg
      541 tcccgggggt gcacgccgtg cgcactgtgg cgccgccgcg cgggcacccg tccgggtcac
      601 ggcccgacgc cgggcggcgg atcatcggcg atgtacaccg aggcgatcga ggtcgcgtcg
      661 ctcagcgcgc cacgcagttc ctggccgagt tcgagaactc gagctggatc acgtaggtca
      721 cgccgtcgtt gccgaccgtc gtgccctgct ggccgttgca gatgtcgccg atctcgccgt
      781 tggtgcggtc gtaccacgcc agcggggccc cgaacgtggt cgccaggccg accgccggat
      841 cggtggtcgc ctcgatcagc tcgtgcgagg ccaccgagtc gaggttgttg acccgctggg
      901 cgttgcggcc gcaaccgccg gcgcagctgc cgccctggtc cgggaccacg ccgtagttga
      961 cgttgacgcc gttgcggacg taggtgccgt ggtaggcgca gaacgtcacg cacgaccggc
     1021 tgccgtcggg cgcggtgatg gtgacgccgg ccgggaagtg caccggatag tagttgctgg
     1081 cgctgggcgg cggcagcgag cccgcggcga acagccggtt cagctcggtg tggatcgccg
     1141 cgtccgacac gctggtcgcc gtgcggttgt cgacgaagcc gttcaccccc gaaccccggc
     1201 cgatcgacga atactgcttg agcatgtcgt acagcgcgct cttggtgacc gccttgtaga
     1261 acgcattgag gttggtctgg aactggacat tggcattcca gaacaccggg tgcacgctga
     1321 cgtgggtcag cgtcgggcca ccgaagaacg tcaggcgagc gccggccggg gccgcgtccg
     1381 cgaccttggc gtcgggatcg tgcatcggca tcagatggac ggcggtgccg tcgtcggcag
     1441 ggccgacatc gtcgtcgggc gcggccatgc aggcggggat cagcagcgcg gtcgagagcg
     1501 ggagaatcga gagccaacgg gattgcgtca tgttggagtc ctcgtgggga gaaactgtcc
     1561 ggcgagcagc taacggtatc gcgtggtgcg cgtcaaccca aacgtaccta tttgtcaaaa
     1621 acaggcagtc gtcacctacg tggtagtcga tgtgggctat cgtgttctcg acgccatccg
     1681 acgcagtgcg aatcgcactc gagggtgtcg accgtcaggg caaacacatt tctatcgcgc
     1741 acagagcaga cccggatccg gaacgatctt gattgtcgat attgtctgga taatgccgat
     1801 tcagatcggc agttccctcc cccaggagca agacatcgct cgggtccgag agctccgcac
     1861 agtaccgccg agcagcgccg agcctgattc tgatatgaac attcacggca gacgaaaaac
     1921 tctgaggccg atcgccgccc ggatggtcgg ttcggtttcg aagccatgac gtgggcgcac
     1981 ggccccgctc cgtaaatgag tcagggcgct gcgcgcggcc aggcgcgctg aaaccggcgt
     2041 gagctcaccc gcgatccgat cgcggcgtgt tcggcgcggg cggaagccac gcggtcagaa
     2101 tgcccccatg gcggccgtgt cagaatgcct cagcgccagc cgagatcacg actgcgttct
     2161 gcatcacgtt tcctcggaaa actgtaagct gagctgcgga tcgcctcagt ttttttcctg
     2221 aatccagacg cggccttggc gatttccgct tgggtttcca tacgattcta tcaggtgcct
     2281 ggttttgcta caggtgttgt tagcgaccgt gcgtgggtgc tcccgaactc cccacgattg
     2341 ttgctgttgt ggaataaccg gttccacaag agcggcggaa tcgttggggt gtgcagtttt
     2401 gacacgcgag aacgccgcac gttaggaccg tcctggacac accacccctc aggaggtcca
     2461 gatgtgggca aaaccaaccg ttttcctctg cgcgtgcatc gcgtggtcgg gatgttcatc
     2521 ggccaccagt gccgccgctg atgatgacgg ttcagatgag acctttgagg tcgccaagac
     2581 ggtcaccgac ccgggtcccc gcggtggcga ccgcggcgcc ggcggcccgt acgaccagct
     2641 aagccaggat gaacagacgt ttttcgcggc ggcccgcgac gtctttgccg aggtggactc
     2701 ggtctccgga aagatcgagg aaggcaaggg cctggggccg accttcaacg gcaacagctg
     2761 cgcccagtgc cacgccgagc ccgacgtagg tggttctagc ccccacccca ccctgggcca
     2821 cgcgcgcatg ccgaacccgc aggtgggtct ggccacgctc gaccgcgcgg ccggcggcgc
     2881 ccagaaggtg cccaaattca tcaccccgga cggcccggtc cgcgaggcgc ggttcgtcaa
     2941 gaacccggac ggcaccgacg atggcggcgt ccacggcctg tacacgatcg ccggccgcac
     3001 cgacgcaccg ggctgcacgc tggcgcagcc caacttcgac aaggagctgg ccaacaacaa
     3061 cgtgatcttc cggatcccga cggcggtgtt cggcctcggc ctgctggaga acgtccccga
     3121 cgacgagctg gtcgcgaacc tggcggcgag cgccgacgcc gccggcaagt cgggatgcgg
     3181 cggcgccggc ggcgtcgccg gcgagctcaa ccgcagcggc aacgacggca cgatcacgcg
     3241 gttcggctgg aaggcccaga acaagtcgct gctggtgttc gccggcgagg cgtacaacgt
     3301 cgagcagggc gtgtcgaacg agctgttcaa caacgagcgc gcggcggtcg ggggctgcgt
     3361 gttcaacgga agccccgagg acgcgaccga caccacgacc ggcggcgccg ccgataccac
     3421 gatgttcgcg gcgttcatgc ggctgtcgca ggacccgcag ccgaccacgg cgtcgccgtc
     3481 ggagctgcgc ggccagaagc tgttcggcac caaggcggat cccggcgtcg gctgcgtgct
     3541 gtgccacacc gacacgctga ccaccggcgc ggtgcgctac accggcatgt cgaacgtcac
     3601 gatccacccg tacaccgacc tcgcgatcca tcacatgggg accaagctcg ccgacggcgt
     3661 cacccagggc gccgcgaccg gggacatgtt ccgcaccgcg ccgctgtggg gcgtcggcaa
     3721 gcggatcttc ttcctccacg acggtcggtc gggaccggcc agcggcggcc tggtcgacgc
     3781 gatccgccag cacggaagca ggggctccga ggccagcgtc gtgatccgca agttcacggc
     3841 cctgccgtcg gccgaccagc aggcgatcat cgacttcctg cggtcgctat agccagccgg
     3901 cgcgctactg cgtgaggtgg cgcagcttgt cggggttgcg caccgtgtag atcgcgacga
     3961 tggcgccgtc cgagacctcg agcgcgatgg tcgcgatctc ggcctcggta ccggcgccgg
     4021 tgcgcagcac gaagcccggc aggccgttga tcgtcgtcgc gatcacctcg tcggggaccg
     4081 gcaggcggcg ctgccggtcg aggccgagca ggaagcgcag gatcttgtcc ctgccgtaga
     4141 tcgggttgag cgcggcgtcg cgcttgccgc cgccgtcggt gtacaggatc gcgtcctcgg
     4201 cgagcagccg cgcgatgccc ggcagatcgc cctgcagcac cgcggcgtgg aacgcgctgg
     4261 cgagccgcgc ggtcgcgtcg ccggtcgcgt ggaacc
//
LOCUS       DASVOR010001420         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_511273,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001420 DASVOR010000000
VERSION     DASVOR010001420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511273"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1236
                     /locus_tag="VF516_27005"
     CDS             325..1236
                     /locus_tag="VF516_27005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111420.1"
                     /translation="MRTWLVLAAAACALAGLTAVAAAQPAQKITIGIYAPSVEFGAAQ
                     ARLAYVQGLARAIEQATGLPTEAQSYANIAALKKDAVDFAIIDGMCYATNLGWKLLAT
                     ATIGGGTTRPWALYSSAGESMQALKGKKLAFIGTGCNDAGFVDNAMLESEVDPAFFGG
                     RSAKPDLTAAVAEVASYKAAQAVFAPVGAGKGLTKVFDTGPVPNPAFVEIGSKLPPAV
                     VERVAASVIGYGAGGAIAGWTRPAREIYTALAARMGKVVKAGVFASADPVRIDAKDAL
                     IEPSTLRDTAVVEVRHHFVRPSSGRME"
     gene            complement(1250..>1771)
                     /locus_tag="VF516_27010"
     CDS             complement(1250..>1771)
                     /locus_tag="VF516_27010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8111421.1"
                     /translation="IAITNAGSTTKPGEQPSHGPLSQAEMHEFLDDPPARAQPAKGPL
                     SQAEMHEFLDDPSDKAQPTGPARPKAATHADSPWVAAARAYNSRHSDYAAEFNELTSY
                     RYLDVHGGGDNPDDGRKLDPKLVASWQEQHGLQADGMVGPLTVAKAREVRAKPSASAA
                     VAVAPQADARPPV"
BASE COUNT          251 a          634 c          626 g          260 t
ORIGIN      
        1 cctcggcggc aacccgcccg agtcgctggt caacctcggc atcatctacg accagctcgg
       61 ccggccgcgc gaggcgttcg acgcctggac caaggccaag gcacgcggcg tccaggtccg
      121 cgacctgcag cgctggatcg acgccaagaa gaggatctat gggttctgag aggaagaccc
      181 gcacagcgct ggcaggtgca gggccgcgcg acgcgacgcg cggaggggtg aatggaccgg
      241 cttgccggtc caaaggggac ggggaagtcc cctctcgatc gaccgatgcg aagaagagga
      301 tctatgggtt ctagggcagt caccatgcgg acctggctcg tcctcgccgc cgccgcctgc
      361 gcactcgccg ggctcaccgc cgtggccgcc gcgcagccgg cgcagaagat cacgatcggc
      421 atctacgcgc cgtcggtcga gttcggcgcg gcccaggccc gcctcgccta cgtccagggc
      481 ctcgcccgcg cgatcgagca ggcgaccggc ctgccgaccg aggcccagtc gtacgccaac
      541 atcgccgcgc tcaagaagga cgccgtcgac ttcgcgatca tcgatggcat gtgctacgcg
      601 accaaccttg gctggaagct gctggcgacc gcgaccatcg gcggcggcac gacccggccg
      661 tgggcgctgt actccagcgc cggcgagtcg atgcaggcgc tcaagggcaa gaagctcgcg
      721 ttcatcggca ccggctgcaa cgacgccggc ttcgtcgaca acgcgatgct ggagtccgag
      781 gtcgatccgg cgttcttcgg cgggcgcagc gccaagccgg atctcaccgc cgcggtcgcc
      841 gaggtcgcgt cgtacaaggc cgcgcaggcc gtgttcgcgc cggtcggcgc cggcaagggc
      901 ctcaccaagg tgttcgacac cggccccgtc cccaacccgg cgttcgtcga gatcggcagc
      961 aagctgccgc ccgccgtggt cgaacgcgtc gccgcctcgg tgatcggcta cggcgccggc
     1021 ggtgcgatcg cgggctggac caggccggcg cgcgagatct acaccgcgct cgccgcccgg
     1081 atgggcaagg tggtcaaggc cggcgtgttc gccagcgccg atccggtccg gatcgacgcc
     1141 aaggacgcgc tgatcgagcc atcgaccctg cgcgacaccg cggtcgtcga ggtccgccac
     1201 cacttcgtgc ggccgtccag cggccggatg gagtagcggc cgctcgcgct cagaccggcg
     1261 ggcgggcgtc ggcctgcggt gccaccgcga ctgccgccga ggcggagggc tttgctctga
     1321 cctcgcgcgc cttggcgacc gtgagggggc cgaccatgcc gtccgcctgc agaccgtgct
     1381 gttcctgcca gctcgcgacc agcttcggat ccagcttgcg gccatcgtcg gggttatccc
     1441 cgccaccgtg cacgtcgagg taccggtagc tggtcagctc gttgaactcg gccgcatagt
     1501 cgctgtgccg gctgttgtag gcgcgggcag ccgccaccca cggcgagtct gcatgcgtcg
     1561 cagccttcgg cctcgccggc ccggtcggct gcgccttgtc cgacgggtcg tcgaggaact
     1621 cgtgcatctc cgcctgcgag agcgggccct tggccggctg cgccctggcc ggcgggtcgt
     1681 cgaggaactc gtgcatctcc gcttgcgata acgggccgtg gctgggctgc tcaccgggct
     1741 tggtcgtcga cccggcgttg gtgatcgcga t
//
LOCUS       DASVOR010001421         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_190174,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001421 DASVOR010000000
VERSION     DASVOR010001421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190174"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /gene="asd"
                     /locus_tag="VF516_27015"
     CDS             complement(<1..662)
                     /gene="asd"
                     /locus_tag="VF516_27015"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00163.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HEX8111422.1"
                     /translation="MEFSKPGSIAAERPRARVGAAYSALVGWGATRSLPPRLRGLAYR
                     AFARAVGANLDEVELDLAGYASLGDFFARRLRHGARVIDPAPGAIIAPCDGAIAARGT
                     AVAGALVQAKGRSYRLAELVADGDLAARLTGGAYATIYLSPRDYHRVHAPVDARVLGY
                     DYLPGSLWSVNPRVAARRDGVIARNERVVFQLDAGALGRVALVMVGAGGVGNIRLAPA
                     LGG"
     gene            complement(851..>1562)
                     /gene="glnA"
                     /locus_tag="VF516_27020"
     CDS             complement(851..>1562)
                     /gene="glnA"
                     /locus_tag="VF516_27020"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829664.1"
                     /note="forms a homododecamer; forms glutamine from ammonia
                     and glutamate with the conversion of ATP to ADP and
                     phosphate; also functions in the assimilation of ammonia;
                     highly regulated protein controlled by the
                     addition/removal of adenylyl groups by adenylyltransferase
                     from specific tyrosine residues; addition of adenylyl
                     groups results in inactivation of the enzyme; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEX8111423.1"
                     /translation="ILWYKHIVKNVARAHGKTATFMPKPLFGDNGSGMHTHQSLWKGG
                     KPLFAGDSYAGLSDTALYYIGGLLKHAGAICAFANPTTNSYRRLVPGYEAPVNLAYSA
                     RNRSATIRIPMYSSSPKAKRLELRSPDPSCNPYLAFAAMMMAGLDGIENRIHPGEPLD
                     KDIYSLSPEELAEVPHVPGSLDASLDALHADRAFLMKGDVFSSDLVDAWIAYKRENEA
                     DHVRMRPVPAEFQLYYDV"
BASE COUNT          235 a          553 c          551 g          223 t
ORIGIN      
        1 ccgccgagcg cgggggccag ccggatgttg ccgaccccgc cggcgccgac catgaccagc
       61 gcgacgcggc cgagcgcccc ggcgtcgagc tggaacacca cccgctcgtt gcgcgcgatc
      121 accccgtcgc gacgcgccgc gacccgcggg ttgaccgacc acagcgagcc cggcaggtag
      181 tcgtagccca gcacgcgagc gtcgaccggc gcgtggacgc ggtgatagtc gcgcggcgac
      241 aggtagatcg tcgcgtacgc ccccccggtg agccgcgctg ccagatcgcc gtcggcgacc
      301 agctccgcga gccggtagct gcgccccttg gcctggacca gcgccccggc gacggcggtg
      361 ccgcgcgccg cgatcgcgcc atcgcagggc gcgatgatcg cgcccggcgc ggggtcgatc
      421 acgcgcgcgc cgtgtcgcag cctgcgcgca aagaagtcac cgagggacgc gtagcccgcg
      481 agatcgagct cgacctcgtc gaggttcgca ccgaccgccc gcgcaaatgc gcgatacgcc
      541 agcccgcgca gccgcggcgg cagggatcgg gtcgcccccc agccgaccag cgcggaatag
      601 gcggcgccga cgcgcgcgcg cggccgttcc gctgcaatgc ttcctggctt gctgaattcc
      661 acgcaggtgc tctggcgctc atgactgcat ggctgaacgc agtccggaat gccaagattc
      721 tgccaaaggg ggcgccgagt tacaagcacc ggcgtcggtt gccgaggccg ggccgggggc
      781 acgatcgacg cccgttcgac gcccgatcga tccccgagcc agcgccggta cggcgctcct
      841 cggcgaccgc ctagacgtcg taatacagct ggaactcggc cggcaccggg cgcatccgga
      901 cgtgatcggc ctcgttctcg cgcttgtacg cgatccacgc atcgaccagg tcagaggaga
      961 acacatctcc cttcatcagg aacgcgcggt ctgcatgtag tgcatcgagc gacgcgtcga
     1021 gcgaccccgg gacgtgcggg acctccgcca gctcctcggg cgacagcgag tagatgtcct
     1081 tgtcgagcgg ctcgcccggg tggatccggt tctcgatgcc gtcgagcccg gccatcatca
     1141 tcgccgcgaa cgccaggtac gggttgcacg acggatcggg cgagcgcagc tcgagccgct
     1201 tggccttggg cgagctcgag tacatcggga tccggatcgt cgccgagcgg ttgcgcgccg
     1261 agtacgcgag gttgaccggc gcctcgtagc ccggcaccag gcggcggtag ctgttcgtcg
     1321 tcggattggc gaacgcacag atcgcgccgg cgtgcttgag caggccgccg atgtagtaca
     1381 gcgcggtgtc gctcaggccg gcgtagctgt cgccggcgaa tagcggcttg ccgcccttcc
     1441 acagcgactg gtgcgtgtgc atgccgctgc cgttgtcgcc gaacagcggc ttgggcatga
     1501 acgtcgcggt cttgccgtgc gcgcgggcga cgttcttgac gatgtgcttg taccagagga
     1561 tc
//
LOCUS       DASVOR010001422         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_482310,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001422 DASVOR010000000
VERSION     DASVOR010001422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482310"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VF516_27025"
     CDS             complement(<1..846)
                     /locus_tag="VF516_27025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8111424.1"
                     /translation="MSQLAPTPGPDSAPGAALSVRFLGVRGSIPSPGPLTTAGGNTAC
                     VEVTAGDTRIILDAGSGLRTLGEERMRSGPRHSTILLSHLHWDHVCGLPFFTPIYVPG
                     HRVEIASGPNGVMPTLDAIRALFRAPFFPVDFADIAGQVSVRELPPTDRFMIGDITIT
                     LARLNHPDPVYGFRLDHGGHAIVYATDTEHFACVDPTLKRLAAGADILIYDAQYTPEE
                     YPTKVGWGHSTWQAGAELARAAGVAQLVLFHHDPNRTDAQLAAIEAEARACFPGTVAA
                     REGLVL"
     gene            complement(1003..>1820)
                     /locus_tag="VF516_27030"
     CDS             complement(1003..>1820)
                     /locus_tag="VF516_27030"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8111425.1"
                     /translation="EGARTMGAQAFAVGNHVGFAGAPDLHTAAHEAAHVVQQRAGVQL
                     KGGVGETGDRYEQHADAVADLVVQGKSAASLLASFAGPATTARAGAAPVQRQPGAGNC
                     DSMPFPKVQNDSPNGSSCRRFDGVTTYKMVSQDRLIASGYKFWVSDGSFDKWVRIDGS
                     SELWVQLPRINAATSEAAIGQLRSIVATRKAQLDEIAALYQAKQSSDPEVAAEAEAEY
                     QQRLDEFPGFDEDYARVPQLRDQVDADHRKAFEQQVQELVKLRDEWDPQSETP"
BASE COUNT          246 a          650 c          645 g          279 t
ORIGIN      
        1 cagcacgagc ccttcgcgcg ccgcgaccgt ccccggaaag cacgcgcgcg cctcggcctc
       61 gatcgccgcg agctgcgcgt cggtgcgatt cgggtcgtga tgaaacagca ccagctgcgc
      121 cacgcccgcg gcgcgcgcga gctccgcgcc ggcctgccac gtcgagtgac cccagccgac
      181 cttggtcgga tactcctcgg gcgtgtactg cgcgtcgtag atcaggatgt cggcgccggc
      241 ggcgagccgc ttgagcgtcg ggtcgacgca cgcgaagtgc tcggtgtcgg tcgcgtacac
      301 gatcgcgtgg ccgccgtggt cgagccggaa cccgtacacc ggatcggggt ggttgagccg
      361 cgccagcgtg atcgtgatgt cgccgatcat gaagcggtcg gtcggcggca gctcgcgcac
      421 cgacacctgc cccgcgatgt cggcgaagtc gaccgggaag aacggcgccc ggaacagcgc
      481 gcggatcgcg tcgagcgtcg gcatcacgcc gttggggccg ctcgcgatct cgacccggtg
      541 ccccggtacg tagatcggcg tgaagaacgg caggccgcac acatgatccc agtgcaggtg
      601 cgacagcagg atcgtcgagt gccgcgggcc gctgcgcatg cgctcctcac ccagcgtgcg
      661 cagccccgag ccggcgtcga ggatgatccg ggtgtctccg gcggtgacct cgacacaggc
      721 ggtgttgccg ccggccgtcg tgagcggccc cggcgacggg atcgagccgc gaaccccgag
      781 gaaccgcacc gagagcgccg cgcccggggc tgagtcggga ccaggggttg gagcgagctg
      841 ggacatgaga acctccctcg cgccttactc agagcagctt cagtgccaca cgggatttcc
      901 acggtttacc agcgcttgac gtgcctcgtg ctccatctcg atgcgcccgg ctggagcgac
      961 tgctccagaa tggagcaccc ggcgcgggga tgtcgtggcg gctcagggcg tttcgctctg
     1021 cgggtcccat tcgtcgcgca gcttcacgag ctcctggacc tgctgctcga acgccttgcg
     1081 gtggtcggcg tcgacctgat cgcgcagctg gggcacccgc gcgtagtcct cgtcgaagcc
     1141 ggggaactcg tcgagccgct gctggtactc ggcctcggcc tcggcggcca cctcggggtc
     1201 gctgctctgc ttcgcctggt agagcgcggc gatctcgtcg agctgcgcct tgcgcgtcgc
     1261 gacgatcgag cgcagctggc cgatcgccgc ctcggacgtc gcggcgttga tccgcggcag
     1321 ctggacccac agctcgctcg agccgtcgat ccgcacccac ttgtcgaagc tgccgtcgct
     1381 gacccagaac ttgtaccccg atgcgatcag ccggtcctgg ctgaccatct tgtaggtcgt
     1441 gacgccgtcg aaccgccggc agctcgagcc gttcggcgag tcgttctgga ccttcgggaa
     1501 cggcatgctg tcgcagttgc cagcgccggg ctggcgctgc accggagctg cgccggcgcg
     1561 ggcggtcgtc gccgggccgg cgaacgacgc cagcagcgac gcggcggact tgccctgcac
     1621 gacgaggtcg gcgaccgcgt cggcgtgctg ctcgtagcgg tcgccggtct cgccgacacc
     1681 gcccttgagc tggaccccgg cgcgctgctg caccacatgc gccgcctcgt gcgccgcggt
     1741 gtgcagatcc ggcgcccccg cgaacccgac gtgattgccg acggcgaatg cctgggcgcc
     1801 catcgtccgc gcgccctcgg
//
LOCUS       DASVOR010001423         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_102922,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001423 DASVOR010000000
VERSION     DASVOR010001423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102922"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            252..836
                     /locus_tag="VF516_27035"
     CDS             252..836
                     /locus_tag="VF516_27035"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8111426.1"
                     /translation="MTIAGNREFDIRGVHHLALVCKDMARTVEFYRDVLGMPLVKTLD
                     LPGGRGQHFFFDIGNGDTLAFFWFPGGPEAAPGVASPSCLPGRGDIRTAHGSMNHLAF
                     NVPAEKFDEYYERLKAKGIDVTPVLNHDDSPTQVSRELTDRVFVRSVYFFDPDGICLE
                     FAAWTKPFTPSDVAHEAAQADGTRAKNLVLHDLR"
     gene            1568..>2374
                     /locus_tag="VF516_27040"
     CDS             1568..>2374
                     /locus_tag="VF516_27040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111427.1"
                     /translation="MTGDWAAVGKMILEGILKLLGIDPAAFYALIGKAEDSIEEIIEN
                     PGAFVGHLVDAVKLGFKQFGANFWTHLKDGLVQWLFGTFADAGIHMPASFDIAGIFDL
                     VCQVLGLTWPRLRGKVVKIIGEKNTERLEFVSKYLQALVTGGFAGLWQQVQQDMSGLW
                     DMVVNGVKDWIIQKVVQQAIIKIATMWNPAGAIIQLIETAWNVYCWVRENAQRIFGLV
                     QAVVDSISNIVAGNIGGAANFIEASLAKLVPIAISLFADLIGLGGIADKIR"
BASE COUNT          388 a          817 c          796 g          373 t
ORIGIN      
        1 cgcggttgtg atcgcgatcg aagcccgccg gcgccccgcc ggtgcgcgtc tcccccaccg
       61 cgccgtagct gaacggcgag ccgcgctggc gctccaggtg tcggcgcacc tcgtcctggg
      121 tgggccgcga caggctccac atccgtgtca cctcacctgc gccgccatcg cgagcctccc
      181 ggcgagtggc cccgaccgcc gggtcgggta aaacatctcg cagctgccag ggcggctcgg
      241 aggacagttc catgacgatc gccggcaatc gcgagttcga catccgtggc gtccaccatc
      301 tggccctcgt gtgcaaggac atggcccgca ccgtcgagtt ctatcgcgac gtgctcggaa
      361 tgccactggt caagacgctc gacctgcccg gcggtcgcgg ccagcatttc ttcttcgata
      421 tcggcaacgg cgataccctg gcgttcttct ggttcccggg cggcccggag gcggcgccgg
      481 gcgtggcgag cccgtcctgc ctgccgggcc gcggcgacat caggaccgcc cacggctcga
      541 tgaaccatct cgcattcaac gtccccgccg agaaattcga cgagtactac gagcggctga
      601 aggccaaggg catcgacgtc acgccggtcc tcaaccacga cgattccccc acccaggtga
      661 gccgcgagct caccgaccgc gtgttcgtgc ggtcggtcta cttcttcgac cccgacggaa
      721 tctgcctcga gttcgcggcg tggaccaagc cgttcacgcc ttccgacgtg gcgcacgagg
      781 ctgcgcaggc cgacggaacg cgcgccaaga acctcgtcct ccatgatctt cgatgagctt
      841 ggatgcgacg tggaatgctc ctcgggagta accggcgcca tgtcggcggc gcgaggaggg
      901 tggtggccgg ccagcgcaac ggcctggccc ggccaccaca gttgagatcg ctgcagtaac
      961 gacaggtatg ccccgtcgtg acatgtctcc gtcgagacat gtcgccgccg cgcggagttg
     1021 caagacgcga tcgcgtacgc gcaacgaggg gagacgccgc gctgcgtggc gtactattcg
     1081 catcgtcctc gaggggagga ccatgtcgag gggtcgatga ccctggagag gtggatccgc
     1141 ggcgaagctg agacggcggc gttcgactgg cgtcagccgg cacgcgaact cgacgtcccc
     1201 ctcgagatcg ctcgcgcgct ctacctccgg gcaatgcagc gcacgatcga tgcgcggcgg
     1261 gtcgagacac tgtatctcgc gtggctgcga gcagcggcct cggcgcgcca cccggcaacg
     1321 ccgcccccta ctccgggtcg ccgcacccag gtgctcgatg gccgaacgct cgccggcgag
     1381 ccgcacgccg acgagttgca gcgactcggt ccgggcaagt ggacgcgcac gctgctcgag
     1441 gcccagcacg acgaggagca gtacccgcgg ctcagcgagg ttgcggcact gcgcgatcag
     1501 ctgtacgcgg cgacggccgg gcgcggcgca ggtactgtgc tcgcggcggg gggtcgcgca
     1561 ggcgctggtg accggcgact gggcggccgt cggcaagatg atcctcgagg gcatcctcaa
     1621 gctcctcggg atcgacccgg cggcgttcta cgcgctgatc ggcaaagccg aggattcgat
     1681 cgaggagatc atcgagaacc caggcgcgtt cgtcggccac ctggtcgacg cggtcaagct
     1741 cggcttcaag cagttcggcg ccaacttctg gacccacctc aaggacggcc tggtccagtg
     1801 gctgttcggc acgttcgccg acgccggcat ccacatgccg gcgagcttcg acatcgccgg
     1861 catcttcgac ctggtctgcc aggtgctcgg cctgacctgg ccgcggctgc gcggcaaggt
     1921 cgtcaagatc atcggcgaga agaacaccga gcggctcgag ttcgtctcca agtacctcca
     1981 ggccctggtc accggcggct tcgccggcct gtggcagcag gtccagcagg acatgtccgg
     2041 cctgtgggac atggtcgtca acggcgtcaa ggactggatc atccagaagg tggtgcaaca
     2101 ggcgatcatc aagatcgcca cgatgtggaa tccggcgggc gcgatcatcc agctgatcga
     2161 gaccgcgtgg aacgtctact gctgggtccg cgagaacgcg cagcggatct tcggcctggt
     2221 ccaggcggtg gtcgactcga tctccaacat cgtcgccggc aacatcggcg gcgcggcgaa
     2281 cttcatcgag gcctcgctcg ccaagctggt cccgatcgcg atcagcctgt tcgccgacct
     2341 gatcggcctg ggcggcatcg ccgacaagat ccgc
//
LOCUS       DASVOR010001424         2703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_482331,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001424 DASVOR010000000
VERSION     DASVOR010001424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2703
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482331"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VF516_27045"
     CDS             <1..347
                     /locus_tag="VF516_27045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111428.1"
                     /translation="KVIAFLPGGERPRLRAHQTLRVTLPGYRGVYLESEVRAISEVIG
                     AAEARGRFLSDRLGDSVPLTGTVVVVEGRLSSPTFEADGETFQLHDGMIGQAEVQLQS
                     RSVLESIIPGLH"
     gene            350..2632
                     /locus_tag="VF516_27050"
     CDS             350..2632
                     /locus_tag="VF516_27050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase domain-containing ABC transporter"
                     /protein_id="HEX8111429.1"
                     /translation="MARREQTEVDATPAEAAGPDPAPPTAAELFPSLEKLARARRGHK
                     VPFVQQLEATDCGAACLAMVLGHLGRDVPLDEVREAAGVSGRDGVDANAIVRAAEWYG
                     LRSRGLALDVESLHYLPPGTILHWEFNHFVVFERVTKNGVEIVDPGMGPRRVSLAKFS
                     ESFTGVALVFEPTDAFEPKRRGRGRLGWYFTQLAGQRHVLSRVVVTSVLLRVFALALP
                     LVTAVIVDRVVPRGDKHLLLVVSSGLAGLLVFQLITNLVRSHLLLQLRTNLDTRLTLG
                     FVDYLSRLPFDFFQRRSSGDLMMRVNNNATVRELLTSNTLSALLDGLLVVGYAVLILV
                     IAPLMGVIVVGLGIVQVSVFYIARRGYRDMMARSLEAQARSQSYLVEMLRGMETLKAA
                     AAEPRAVERWSNLYVDELNVALDRGRLSARVDAVGSLLTTGSPLTVLAIGALQVMSGA
                     ITLGEMLAINALAIGLLTPLSTMISSALQLQLLGGYMDRIDDVLRTAPEQTGTDVARA
                     PRLTGRVTLQNVSFRYGDNLPFVVRDVSVDIRAGMTVAIVGRSGCGKSTLARMIAGLY
                     RPTEGRVLFDGHDLARLDLKSLRRQIGVVFQSPSLFAGSIRAAIALSDPTANLDRITD
                     AARRAVVDEDIRAMPMSYDTILADGGASMSGGQRQRVALARALVHNPALLILDEATSA
                     LDSETERRVIRNLEDLRCTRIVLAHRLSTIVNADLILVMDAGEIVETGTHGELLAHGR
                     HYARLVAAQLQAEPAQRGVA"
BASE COUNT          344 a         1042 c          918 g          399 t
ORIGIN      
        1 tcaaggtgat cgcgttcctg cccggcggcg agcggccgcg gctgcgcgcc caccagacgc
       61 tgcgcgtcac cctgcccggc taccgcgggg tctacctcga gagcgaggtc cgcgcgatct
      121 ccgaggtgat cggcgccgcc gaggcgcgcg ggcggttcct cagcgaccgg ctcggtgaca
      181 gcgtgccgct gaccggcacg gtggtcgtgg tcgagggccg gctgagctcg ccgacgttcg
      241 aggccgacgg cgagaccttc cagctccacg atggcatgat cggccaggcc gaggtccagc
      301 tgcagtcgcg ctcggtgctc gagagcatca tcccggggct acactagtta tggcgcgccg
      361 agagcaaacc gaggtcgatg ccacccctgc ggaggcggcg ggccccgacc ccgcgccgcc
      421 gaccgcggcc gagctgttcc cgtcgctcga gaagctcgcc cgcgcgcgcc gcggccacaa
      481 ggtgccgttc gtccagcagc tcgaggccac cgactgcggc gccgcctgcc tggccatggt
      541 gctcggccac ctcggccgcg acgtgccgct cgacgaggtc cgcgaggccg ccggggtcag
      601 cgggcgcgac ggcgtcgacg ccaacgcgat cgtgcgcgcc gccgagtggt acggcctgcg
      661 cagccgcggc ctcgcgctcg acgtcgagag cctgcactac ctgccgcccg gcacgatcct
      721 gcactgggag ttcaaccact tcgtcgtgtt cgagcgggtc accaagaacg gcgtcgagat
      781 cgtcgacccc gggatgggcc cgcgccgggt ctcgctcgcc aagttcagcg agtcgttcac
      841 cggcgtcgcc ctggtgttcg aaccgaccga cgcgttcgag cccaagcgcc gcggccgcgg
      901 ccggctcggc tggtacttca cccagctcgc cggccagcgc cacgtgctgt cgcgcgtggt
      961 cgtcacctcg gtcctgctcc gcgtgttcgc gctcgccctg ccgctggtca ccgcggtgat
     1021 cgtcgaccgc gtcgtgccgc gcggcgacaa gcacctcctg ctcgtggtgt cgagcggcct
     1081 cgccggcctg ctggtgttcc agctgatcac caacctggtg cgctcgcacc tcttgctcca
     1141 gctgcgcacc aacctcgaca cccggctcac gctcggcttc gtcgactacc tgtcgcggct
     1201 gccgttcgac ttcttccagc gccggtcgtc gggcgacctg atgatgcggg tcaacaacaa
     1261 cgccacggtg cgcgagctgc tcaccagcaa cacgctgtcg gccctgctcg acggcctgct
     1321 ggtcgtcggc tacgccgtgc tgatcctggt gatcgccccg ctgatggggg tgatcgtcgt
     1381 cgggctcggc atcgtccagg tctcggtgtt ctacatcgcg cggcgcggct atcgcgacat
     1441 gatggcgcgc tcgctcgagg cccaggcgcg ctcgcagtcg tacctggtcg agatgctgcg
     1501 cggcatggag accctcaagg ccgccgccgc cgagccgcgc gccgtcgagc gctggtccaa
     1561 cctctacgtc gacgagctca acgtcgcgct cgaccgcggc cggctcagcg cgcgggtcga
     1621 cgcggtcggc agcctgctca ccaccggctc accgctcacc gtgctggcga tcggcgcgct
     1681 ccaggtcatg tccggcgcga tcacgctcgg cgagatgctc gcgatcaacg cgctcgcgat
     1741 cggcctgctc accccgctgt cgaccatgat cagcagcgcg ctccagctcc agctgctcgg
     1801 cggctacatg gaccggatcg acgacgtgct gcgcaccgcg cccgagcaga ccggcaccga
     1861 cgtcgcgcgc gccccgcggc tgaccggccg ggtcacgctg cagaacgtgt cgttccgcta
     1921 cggcgacaac ctgccgttcg tcgtccgcga cgtctcggtc gacatccgcg ccgggatgac
     1981 ggtcgcgatc gtcggccgct cgggctgcgg caagtcgacg ctcgcccgca tgatcgccgg
     2041 gctgtatcgc ccgaccgagg gccgcgtgct gttcgacggc cacgacctcg cccggctcga
     2101 cctcaagtcg ctgcgccgcc agatcggcgt ggtgttccag tcgccgtcgc tgttcgccgg
     2161 ctcgatccgc gcggcgatcg cgctcagcga tccgaccgcc aacctcgacc ggatcaccga
     2221 cgccgcccgc cgcgcggtgg tcgacgagga catccgcgcg atgccgatga gctacgacac
     2281 gatcctcgcc gacggcggcg cgtcgatgtc gggcggccag cgccagcgcg tcgcgctcgc
     2341 ccgcgccctg gtccacaacc ccgcgctgct gatcctcgac gaggcgacca gcgcgctcga
     2401 cagcgagacc gagcgccgcg tgatccgcaa cctcgaggac ctgcggtgca cccggatcgt
     2461 gctcgcccac cggctgagca cgatcgtcaa cgccgacctg atcctcgtga tggacgccgg
     2521 cgagatcgtc gagaccggca cccacgggga gctcctggcg cacggccggc actacgcccg
     2581 gctggtcgcc gcccagctcc aggccgagcc cgcccagcga ggggtcgcat gaccgggcgc
     2641 gccgtcggtg tgctcgccct cgggctcgcc gtcgggctcg cgcccggctg ccgcggcggc
     2701 ggc
//
LOCUS       DASVOR010001425         1932 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_335743,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001425 DASVOR010000000
VERSION     DASVOR010001425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1932
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335743"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            445..1353
                     /locus_tag="VF516_27055"
     CDS             445..1353
                     /locus_tag="VF516_27055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111430.1"
                     /translation="MLTSMGARSLQTAYMAGARVCSMNDFRRMGPSGLELWLATHGAG
                     ASPVLDEICAQKDAHTARLYWFTELPAAIVEAQRTQRPILSLRLLGRLDEELSCANSR
                     FFRKLLYPDPRINQVLRNDFVLHWQSVRPVPKITIDFGDGRWIERTLTGNSAHVVLDM
                     HGRAADALPGLMSREMFLAQLIQARSLALARRDQLAALHALAGSWLRVVGSVSRAVTA
                     SRIAPSKHRIEAPLLRAVELDLAADTAQNLELYRRIHEAFAARAEWAGLDGFVEWIYA
                     ELFEMPPGDAALGLDVPDPFPLAAAG"
     gene            complement(1398..>1932)
                     /locus_tag="VF516_27060"
     CDS             complement(1398..>1932)
                     /locus_tag="VF516_27060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111431.1"
                     /translation="TLMAIVHREVQRLNVLIGDLLDYANPRPPQLVDFDLGVMVEETL
                     QVARREPAFASVEMAFSVDRPLPLHADPAKLRQVLWNLLRNAADAAALGGHHLRVDAR
                     REPDATTLVVADDGPGIPADQLTRIFDPFFTTKTKGTGLGLATCHAIVAEHGGHIDAA
                     SEPGKGTRMTVSLPREP"
BASE COUNT          254 a          680 c          704 g          294 t
ORIGIN      
        1 ggctctctcg gccgcgcgca tggcgagcgc gatccgctgg cgccaggccg caccggccgt
       61 ggcggctgtc acccatgtgg ggcgttgctg tccgcggtcg gctgcgagcg gttgcagcgg
      121 gagactggca caggctggca cccacgtggg ggcgttgctg tccgcggtga cgaagcggtg
      181 cccccggcga ttccgcggaa tcgtggtcgg ctccctcggc cgcgcgcatg gcgagcgcga
      241 tccgctggca gcaggccaca ccggcggtgg cggctgtcag ccgtgggggg cgttgctgtt
      301 cgcggtggcg gtgagctgcg tcgtcggttc cacctccagc ggcggccgcc gtgctcggcc
      361 tgtggtgcgt ccacgaggaa ccaccaaggt cgccctgccg cttgccgaag cgcggccacg
      421 aacccgatcc attcgatcac ctacgtgctc accagcatgg gggccagatc cttacaaacg
      481 gcttacatgg ccggcgctag ggtttgcagc atgaacgact tccgacgcat gggccccagc
      541 ggcctcgagc tctggcttgc aacgcacggc gccggggcat ccccggtact cgacgagatc
      601 tgcgcccaga aggacgccca cacagcacgg ctgtactggt tcaccgagct gcccgccgcg
      661 atcgtcgagg cgcagcgaac gcagcgcccg atcctgtcgc tgcggctgct gggccggctc
      721 gacgaggagc tgtcgtgcgc caacagccgg ttctttcgca agctgctgta cccggatccc
      781 cggatcaacc aggtgctgcg gaacgacttc gtgctgcact ggcaatcggt ccgtccggtc
      841 ccgaagatca cgatcgattt cggcgacggc cgctggatcg agcggacgct gaccggcaac
      901 agcgcgcacg tcgtgctcga catgcatggg cgcgcggccg atgccctgcc gggcctgatg
      961 tcgcgcgaga tgttcctcgc gcagctgatc caggcgcgat cgctggcgct cgcccgtcgc
     1021 gaccagctcg ccgcgctgca tgccctcgct ggctcctggc tgcgggtcgt tggatcggtg
     1081 tcgcgcgcgg tcacggccag ccggatcgcg ccgagcaagc accgcatcga ggctccgctg
     1141 ctgcgcgcgg tcgagctcga cctcgcggcc gacaccgccc agaacctcga gctgtaccgc
     1201 cggatccacg aggcgttcgc cgcccgcgcc gaatgggccg ggctcgacgg cttcgtcgag
     1261 tggatctacg ccgagctgtt cgagatgccg cccggcgacg ccgcgctcgg gctcgatgtg
     1321 ccggacccgt tcccgctcgc cgcggcggga tgaccggccg gcccaggagg cgaccgccgg
     1381 ttcgaagact cgccggctca gggctcgcgc ggcaggctga ccgtcatccg ggtgcccttg
     1441 ccgggctcgc tggcggcgtc gatgtggccg ccgtgctcgg cgacgatcgc gtgacaggtc
     1501 gccagcccca gccccgtgcc cttggtcttg gtggtgaaga acgggtcgaa gatccgggtg
     1561 agctggtcgg cggggatccc ggggccgtcg tcggccacga ccagcgtcgt ggcgtcgggc
     1621 tcgcgccgcg cgtcgacccg gaggtggtgg ccacccagcg cggcggcgtc ggccgcgttg
     1681 cggagcaggt tccacagcac ctggcgcagc ttggcagggt cggcgtggag cggcagcggc
     1741 cggtcgaccg agaacgccat ctcgacgctg gcaaacgccg gctcgcggcg cgcgacctgc
     1801 agggtctcct cgaccatcac gccgaggtcg aagtcgacca gctgcggcgg ccgcgggttg
     1861 gcgtagtcga gcaggtcgcc gatcagcacg ttgagccgct ggacctcgcg gtggacgatc
     1921 gccatcagcg tg
//
LOCUS       DASVOR010001426         6398 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_102954,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001426 DASVOR010000000
VERSION     DASVOR010001426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6398
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102954"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VF516_27065"
     CDS             complement(<1..1116)
                     /locus_tag="VF516_27065"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8111432.1"
                     /translation="MATDGVEAATYLARAGALLERFDPTISLQAIVDLAVPVLADWCF
                     IHLKTAEHPRIVAIAHADPDQLAAARARAAIPRPLSADTAVARVLAGGPPEIVQVDPD
                     VIVRAARDPAHHEQLRLQGYRSAVVAPLVGRDGVLGAVTFTMAESGRVYEAGDLAMLT
                     ELARRTGIALDNARLFAAEQEARRHAEEARDRTRRLQNLTAMLSSALDQHRVVSILVD
                     AGRSALGAGAGFAWLLRDPDTLELAGWELAGRSSRLDGFRLIPMTARMPVCDVVRTTQ
                     PMMFENLTAMTASYPDAVPPGDSPFRAWAVIPLVVGGRGLGGLSFSFAGERAFSADDR
                     ELLAELRSWTTRPAPLRIRARRRRDDLPPPRIIRYGNH"
     gene            1664..3079
                     /locus_tag="VF516_27070"
     CDS             1664..3079
                     /locus_tag="VF516_27070"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8111433.1"
                     /translation="MIELEFLGGAQTVTGSKHLVRTSRAVVLLECGLFQGHRAEANRR
                     NRSLPVDPAALDAVVLSHAHLDHSGALPVLVHHGYRGPIYATPATRDLCAPMLDDAAS
                     IQLADARHIDRLIARGVDIDPVTPLYDHDDVVAALTQMIGLPYHHGQAIAPGVELTLL
                     DAGHVLGSAIVVLDVDDGGTRVRIAFTGDLGRPNMPILRDPEIPEHVDTLITESTYGD
                     RHHAPIADAQDELAAVISRTAARGGKLVIPSFALERAQEIIFALGRLRGRGALPALPI
                     YVDSPLATKLTEVFRLHPDCYDRDAFAQLQTGSLFEFDGLRYVTEVEDSKAIDAAPGP
                     AVVIAGSGMCEGGRVLHHLRALVSDPRNTIAIVGFQAEHTLGRRIAERRPQVKIFGVT
                     HELHAEVVVFDGFSAHADQAGLIEFAEAVRRQQRRGALRGVFLVHGEPPAQAALAAKL
                     TARGFPCVASPQGDRVELARA"
     gene            3323..>6398
                     /gene="hrpA"
                     /locus_tag="VF516_27075"
     CDS             3323..>6398
                     /gene="hrpA"
                     /locus_tag="VF516_27075"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEX8111434.1"
                     /translation="MAPPPSISPPANLARSAGAPSAMVLRSGLRLSDRIEFPPELPIT
                     ERIVDIANAIDAHPVVIVAGETGSGKTTQLPKICLAMGRGVDRQIGCTQPRRIAATSV
                     AARVAQELATELGDVVGYKVRFTDKVRATSSVKFMTDGILLAEIQGDAKLRRYDTIIV
                     DEAHERSLNIDFLLGFLRRLLPERPDLRVIVSSATLEIERFAAFFGDAPVIEVSGRTY
                     PVDVLYRPPRDDEADLADAVANTVNEIAEMDPRGDMLVFLPGEREIHEAAAEIEKRAL
                     PHTMVLPLYARLPASEQQRVFQRLPERRVVLATNVAETSLTIPGIVYVVDPGLARVNR
                     YNVRTGVSQLLIEPISKASADQRKGRCGRTANGVCFRLYDEQDYTSRPGHTDPEIKRI
                     SLAGVILRMKALRLGDIERFPFLDPPQPRAIADGYRVLEELGAIRDDGRLTAIGEQLG
                     RLPLDPRLGRMILGGRDEGALREVLILAAVLGLQDPRERPAAVLQRADEAHRKFRDEA
                     SDFASYLSLWSFWQDARAHSSRRQIQKLCRDHFLSYPRMREWEDIHDQLVRVMRELGF
                     SPGAPASADQIHRALLPGLLSRIGMWSTEARIYIGARQTRFAIHPSSGLARKPPAWVM
                     AAELVETSQLFARGVARIDPAWLEKAAGPLCRRNHGDPHWEQKQGQVMAKEHVSLYGL
                     PIVKDRKVPYAPIDPALCRTMFITHALVRHEYATRGAFMEHNRRLLDEVHRLRDKARK
                     SDMVADEYELGQFFDRRIPDGVVSGKTFEDWRKDAEARDPAILELALSDLLLDEAGEL
                     TPERYPDQLAVCGVALALVYTFDPSADDDGITAIVPLAVLPQLDPEVLAWTIPGWHET
                     KLAALLEALPKTVRKALAPLDELARDLAARLRPFEGPMLPALERAILERTGERVPRDA
                     WDLHALPAYLGFQFRVVDERDKVLGEGRDLADLQRTLGQRARQLWAAVPRERYERSGL
                     VAWDIGELPASVPLEVGGRRLQAYPALVDRDTAVDVRLLESSAAAAEATRAGL"
BASE COUNT          907 a         2314 c         2288 g          889 t
ORIGIN      
        1 gtggttgccg tagcggatga tgcggggagg agggagatcg tcgcggcggc ggcgcgcgcg
       61 gatgcgcagg ggcgccggac gcgtcgtcca cgagcgcagc tcggcgagca gctcgcggtc
      121 gtcggcgctg aacgcgcgct cccccgcgaa cgagaacgac aggccgccga ggccgcggcc
      181 gccgaccacg agcgggatca cggcccaggc ccgaaacggc gagtcgccag gtgggacggc
      241 atccggatac gacgcggtca tcgcggtcag gttctcgaac atcatcggtt gcgtggtgcg
      301 cacgacatcg cagaccggca tccgcgcggt catcgggatg agccggaagc cgtcgagccg
      361 gctgctccgc ccggcgagct cccagccagc gagctcgagc gtgtcgggat cacgcagcag
      421 ccaggcgaac ccggcgccgg cgccgagcgc gctgcgtccg gcgtcgacca ggatcgacac
      481 gacgcggtgc tggtcgagtg cgctcgacag catcgcggtc aggttctgga gccgccgggt
      541 ccggtcgcgt gcttcctcgg cgtggcgtcg cgcctcctgc tcggcggcga acagccgcgc
      601 gttgtcgagc gcgatcccgg tgcgccgcgc gagctcggtg agcatcgcga ggtcgccggc
      661 ctcgtagacc cgcccggact cggccatcgt gaaggtcacc gcgccgagca cgccgtcgcg
      721 gccgaccagt ggcgcgacga ccgccgagcg atacccctgc aggcgcagct gctcgtggtg
      781 cgcaggatcg cgcgccgccc ggacgatcac gtcggggtcg acctggacga tctcgggcgg
      841 cccgccggcg agcacgcgcg cgacggcggt atcagcggac agcggccgtg ggatggcggc
      901 ccgggcgcgc gccgcggcga gctggtcggg atccgcgtgc gcgatcgcga cgatcctcgg
      961 gtgctccgcg gtcttgaggt gaatgaagca ccagtcggca agcaccggca ccgcgagatc
     1021 gacgatcgcc tgcagcgaga tcgtcggatc gaagcgctcg agcagcgccc cggctcgggc
     1081 aaggtaggtg gcggcctcga caccgtccgt ggccatcgct gcgtgaccac ggaagtcgag
     1141 atgtctcaca ttcaagatcg tggcgcggtc gctcgatatc cgtcaacacc cacgcaagca
     1201 gtgtgcgagg gcgccacatg agcgctgtgg ccacagtgtc gggccggcgt caggccacgg
     1261 ccctgctgcg agcgttggcc cacgcctcct cgaacgctgc gacctgatcg gcgacgttgc
     1321 ccggatcgag gatgatcgcg ccattctcga cgagcttgga gtcgaacgcc gcgccgctcc
     1381 ggccgctctg gtagccgttg aacgaggcgg gcccatgtag gtctgctcgt gacgaccgcc
     1441 gcagtcgccg ccgaggccga acccggcggt gcggcagatc tccaggcgcg gaatacgcac
     1501 ggcttgcgtt tcctcgtgca tgcgcgcaac cggatcgcga acgaggcatg caccacgcgc
     1561 agcccgcgtc gcgcccccgg cagagctccg ctgccttgcg cctgtcacag gtcggcggat
     1621 gccatgcggg cggcccggca cgtcgcgtgc ttcgagggtg gacatgatcg agctcgagtt
     1681 cctcggcggc gcgcagaccg tgaccggctc caagcacctg gtccggacgt cgcgcgccgt
     1741 ggtgctgctc gagtgcggcc tgttccaggg ccaccgcgcc gaggccaacc ggcgaaatcg
     1801 ctcgctgccg gtcgaccccg cagcgctcga tgccgtcgtg ctgtcgcacg cgcacctcga
     1861 ccattccggg gcgctgccgg tgctcgtcca tcacggctac cgcggcccga tctacgccac
     1921 cccggcgacg cgcgatctgt gcgcgccgat gctcgacgat gccgcgagca tccagctcgc
     1981 cgacgcccgg cacatcgacc ggctgatcgc ccgtggtgtg gacatcgatc cggtcactcc
     2041 gctctacgat cacgatgatg tggtcgccgc gctgacgcag atgatcggcc tgccgtatca
     2101 ccacggtcag gcgatcgcac cgggtgtcga gctgacgttg ctcgatgccg gccacgtgct
     2161 cggcagcgcg atcgtggtgc tcgacgtcga cgacggcggc acccgggtgc gcatcgcatt
     2221 caccggcgac ctcggtcgac cgaacatgcc gatcctgcgc gacccggaga tccccgaaca
     2281 cgtcgatacc ctgatcaccg agagcacgta tggcgatcgc caccatgccc cgatcgccga
     2341 tgcgcaagac gagctggccg cggtgatatc gcgcaccgcg gcgcgcggcg gcaagctcgt
     2401 gatcccgagc ttcgcgctcg agcgtgcgca ggagatcatc ttcgcgctcg gccgtctgcg
     2461 cggccgcggc gccctgcctg ccctgccgat ctacgtcgac tcaccgctcg ccaccaagct
     2521 gaccgaggtg ttccggctcc acccggactg ctacgaccgc gatgcgttcg cgcagctgca
     2581 gaccggctcg ctgttcgagt tcgatggcct gcgatacgtg accgaggtcg aggactccaa
     2641 ggcgatcgac gcggcgcccg gccccgcggt cgtgatcgcg ggcagcggaa tgtgcgaggg
     2701 cggccgggtg ctgcatcacc tgcgcgcgct ggtctccgat ccgcgcaaca cgatcgcgat
     2761 cgtcggcttt caggccgagc acacgctcgg gcgccggatc gccgaacgcc gcccacaggt
     2821 gaagatcttc ggggtgacgc acgagctgca tgcggaggtc gtcgtgttcg atggcttctc
     2881 ggcgcacgcc gatcaagcgg ggctgatcga gttcgccgag gcggtccgcc ggcagcagcg
     2941 gcgaggagcg ctgcgcggcg tgttcctggt ccacggcgag ccccccgcgc aggccgcgct
     3001 cgccgcgaag ctcaccgcgc ggggcttccc ctgcgtggct tccccgcagg gagatcgtgt
     3061 cgagcttgcg cgagcatgag ccgggccggg acgccgccgg tggccgccgt ctggccttcc
     3121 cggcggcggc tcgtccgatc gcgagacgca cctgacgggg cgaagcaacg cgccggtcgc
     3181 aatcgactcg aaccggcagg gcgcggcaag ccgcgacctc gctggctcgc cgcggcggcg
     3241 cggcggtcgc cagcgccgcg cggattcgct aggctcgccc acccccgcgc tgacccgatc
     3301 acgcctcgcc gccgctgcct gtatggcccc ccctccctcg atctcgcccc ccgcgaacct
     3361 cgcgcgcagc gccggcgcgc cgtcggcaat ggtgctgcga tcggggctgc ggctcagcga
     3421 ccggatcgag tttccaccgg agctgccgat caccgagcgg atcgtcgaca tcgccaacgc
     3481 gatcgacgct cacccggtgg tgatcgtggc cggcgagacc ggctcgggca agacgaccca
     3541 gctcccgaag atctgcctgg cgatgggccg gggcgtggac cggcagatcg gctgcacgca
     3601 gccgcggcgg atcgcggcga ccagcgtcgc ggcgcgcgtg gcgcaggagc tcgcgaccga
     3661 gctcggcgac gtggtcggct acaaggtgcg cttcaccgac aaggtccggg ccacgtcgtc
     3721 ggtgaagttc atgaccgacg ggatcttgct cgccgagatc cagggcgacg ccaagctgcg
     3781 gcgctacgac acgatcatcg tcgacgaggc ccacgagcgc agcctgaaca tcgacttcct
     3841 gctcggcttc ctccgccggc tgttgcccga gcggcccgac ctgcgcgtga tcgtcagctc
     3901 ggcgacgctc gagatcgagc ggttcgccgc gttcttcggc gacgcgcccg tgatcgaggt
     3961 atcgggccgg acctacccgg tcgacgtgct gtaccgcccg ccgcgcgacg acgaggcgga
     4021 cctcgcggac gcggtcgcca acacggtcaa cgagatcgcc gagatggatc cgcgcggcga
     4081 catgctggtg ttcctgccgg gcgagcgcga gatccacgag gcggcggccg agatcgagaa
     4141 gcgagcgctg ccccacacca tggtgctgcc gctctacgcg cggctgcccg cgtccgagca
     4201 gcagcgggtg ttccagcggc tgcccgagcg ccgggtcgtg ctggcgacca acgtcgcgga
     4261 gacctcgctc acgatccccg ggatcgtgta cgtcgtcgac ccggggctcg cgcgcgtcaa
     4321 ccgctacaac gtccgcaccg gcgtgtccca gctgctgatc gagccgatct cgaaggccag
     4381 cgccgaccag cgcaagggcc gctgcgggcg caccgcgaac ggtgtgtgct tccggctgta
     4441 cgacgagcag gactacacgt cgcggcccgg ccacaccgat cccgagatca agcggatcag
     4501 cctcgccggg gtgatcctgc gcatgaaggc gctgcggctc ggcgacatcg agcggtttcc
     4561 gttcctcgat ccgccccagc cgcgcgcgat cgcggacggc taccgggtgc tcgaggagct
     4621 cggcgcgatc cgcgacgacg gccggctgac ggcgatcggc gagcagctgg gccggttgcc
     4681 gctcgacccg cggctcgggc ggatgatcct cggcggccgc gacgagggcg cgctgcgcga
     4741 ggtgctgatc ctcgccgccg tgctcggcct gcaggatccg cgcgagcggc cggcggcggt
     4801 gctgcagcgc gccgacgagg cgcaccgcaa gttccgcgac gaggccagcg acttcgcgag
     4861 ctacctgagc ctgtggagct tctggcagga cgcgcgcgcg cacagctccc gccggcagat
     4921 ccagaagctg tgccgcgatc acttcctgtc gtacccgcgg atgcgcgagt gggaggacat
     4981 ccacgatcag ctggtgcgcg tgatgcgcga gctcgggttc tcgcccggcg cgccggcgtc
     5041 ggccgatcag atccaccgcg cgctgctgcc gggcttgctc agccggatcg ggatgtggag
     5101 caccgaggcg cggatctaca tcggcgctcg ccagacccgc ttcgcgatcc atccgtcgtc
     5161 ggggctcgcc cgcaagccgc cggcgtgggt gatggcggcc gagctggtcg agacctcgca
     5221 gctgttcgcg cgcggcgtgg cgcggatcga tccggcgtgg ctcgagaagg ccgccgggcc
     5281 gctgtgccgg cgcaaccacg gcgatccgca ctgggagcag aagcagggcc aggtcatggc
     5341 caaggagcac gtctcgctgt acggcctgcc gatcgtcaag gaccgcaagg tcccctatgc
     5401 gcccatcgat ccggcgctgt gccgcacgat gttcatcacg cacgccctgg tccgccacga
     5461 gtacgcgacg cgcggggcgt tcatggagca caaccgccgc ctgctcgacg aggtgcaccg
     5521 gctgcgcgac aaggcgcgca agagcgacat ggtcgccgac gagtacgagc tcggccagtt
     5581 cttcgaccgg cggattcccg acggcgtggt cagcggcaag acgttcgagg actggcgcaa
     5641 ggacgccgag gcccgggatc cggcgatcct cgagctcgcg ctgtccgacc tcctgctcga
     5701 cgaggccggc gagctgacgc ccgagcgcta tcccgatcag ctcgccgtct gcggcgtcgc
     5761 gctggcgctg gtctatacgt tcgatccgag cgcggacgac gacggcatca ccgcgatcgt
     5821 gccgctcgcg gtcctgccgc agctcgatcc ggaggtcctg gcgtggacca tccccggctg
     5881 gcacgagacc aagctcgccg cgctgctcga ggcgctgccc aagaccgtgc gcaaggcgct
     5941 ggctccgctc gacgagctcg cgcgcgacct cgccgcccgg ctgcgtccgt tcgagggtcc
     6001 gatgctaccg gcactcgagc gcgcgatcct ggagcgcacc ggcgagcgag tgccgcgcga
     6061 cgcctgggac ctgcacgcgc tgcccgcgta cctggggttc cagttccgcg tcgtcgacga
     6121 gcgcgacaag gtcctcggcg agggccgcga tctcgccgat ctccagcgca cgctcggcca
     6181 gcgcgcccgg cagctgtggg ccgcggtgcc gcgcgagcgc tacgagcgct ccgggctcgt
     6241 ggcgtgggac atcggcgagc tcccggcctc ggtccccctc gaggtcggcg ggcgcaggct
     6301 gcaggcgtac cccgccctcg tcgaccggga caccgcggtc gacgtgcgcc tgctcgagtc
     6361 gtccgcggcg gcggccgagg ccacccgcgc gggcctgc
//
LOCUS       DASVOR010001427         1683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_482367,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001427 DASVOR010000000
VERSION     DASVOR010001427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482367"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..1435)
                     /locus_tag="VF516_27080"
     CDS             complement(146..1435)
                     /locus_tag="VF516_27080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011599256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEX8111435.1"
                     /translation="MLKVVQEENWTNAASGSTASSSLLDEIVRDGARQILAAALRAEI
                     AAYIAAHADQVDENGHRLVVRNGYHAEREVTTAAGAVPVRQPRVNDKRVDPTTGERMR
                     FSSAILPAWCRKSPQVTEVLPLLYLHGLSTSDFTPALERFLGTSHGLSASVITRLTGQ
                     WKDEATRFAQRSLTDVDYVYLWVDGIHLKVRLEQDKVCLLVMIGVRADGTKQLVALAD
                     GFRESAESWADLLRDCKRRGMRAPVLAVGDGALGFWKAIRDVFPQTREQRCWWHKIGN
                     VLAALPKSAHPGAKKALAEIYNAENRQHAERAAKAFAAEYGAKWPKAVAKITDDLDVL
                     LAFYDYPAEHWIHLRTTNPIESTFATVRLRQRVTKGPGSRAAGVAMAFKLIESAEHRW
                     RAVNAPNLVALVRAGARFENGKLIERPDESGGETQAA"
     gene            complement(1495..>1683)
                     /locus_tag="VF516_27085"
     CDS             complement(1495..>1683)
                     /locus_tag="VF516_27085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111436.1"
                     /translation="HLVQERVLCGSDLSLVSTELLFCEFNGAKQVPNGQQEQRSMIRT
                     CGCPRWGDVNGRALPSKI"
BASE COUNT          264 a          574 c          556 g          289 t
ORIGIN      
        1 cgcgacgttc gccgactcaa gcaggcggtg gaggtcatta gccacatcgt cgaggaggcg
       61 cttgcgcatg ggcgccggat cgctgttccc aagaccagcg cggtcgaagg cgcagaggaa
      121 tagtcaagac ctgtggatcg gcgtgtcacg cggcttgagt ttcgcctcct gattcgtcgg
      181 gtcgttcgat gagcttgccg ttctcgaacc gggcgccggc tcggacgagg gcgaccaggt
      241 tgggtgcgtt cacggcacgc cagcggtgct cggcggactc gatgagcttg aacgccatcg
      301 cgacgccggc ggcccgggag cccggtcctt tggtgacccg ctgccgcagc cgcacggtcg
      361 cgaaggtgga ctcgatcggg ttggtggtgc gcaggtggat ccagtgctcg gccgggtagt
      421 cgtagaaggc cagcagcacg tcgaggtcgt cggtgatctt cgcgaccgcc tttggccact
      481 tcgcgccgta ctcggcggcg aacgccttcg cagcccgctc ggcgtgctgt ctgttctcgg
      541 cgttgtagat ctccgccagc gccttcttcg cccccgggtg cgcggacttg ggcagtgcgg
      601 cgaggacgtt gccgatcttg tgccaccagc accgctgctc gcgagtctgc gggaacacat
      661 cccggatcgc cttccagaag cccaacgctc cgtccccgac ggccagcacg ggagcgcgca
      721 tgccgcggcg cttgcagtcg cgcagcaggt cggcccagga ctcggccgat tcgcggaagc
      781 cgtcggccag cgcgaccagc tgcttggtgc catcggcgcg gaccccgatc atcaccagca
      841 ggcagacctt gtcctgctcc aggcgcacct tgaggtggat cccgtcgacc cacagataga
      901 cgtagtcgac gtcggtcagc gaccgctggg cgaacctggt ggcctcgtcc ttccactggc
      961 cggtcagccg agtgatcacc gaggcagaca gtccgtggct ggtgcccagg aaccgctcca
     1021 acgccggggt gaagtccgac gtggataggc catgcaagta cagaagcggc agcacctccg
     1081 tcacctgggg cgacttcctg caccaggccg gcaggatcgc cgaggagaac cgcatccgct
     1141 cacccgtggt cgggtcgacg cgcttgtcgt tgacccgcgg ctgccggacc ggcaccgcac
     1201 ccgccgcggt ggtcacctcc cgctccgcgt ggtaaccgtt gcggaccacc agccggtgcc
     1261 cgttctcgtc gacctgatcg gcgtgagcag cgatgtaggc agcgatctcg gcccgcagcg
     1321 ccgccgcaag tatctgccgg gcgccgtccc ggacgatctc atccagcagc gacgagctcg
     1381 ccgtcgaccc gctcgccgcg ttggtccagt tctcctcctg cactaccttg agcatgggcg
     1441 tgccctccca agccggcgcg ccaacgccga ccctgatcag aacgtgcatg ggcttcagat
     1501 cttgctcggg agggcgcgcc cgttcacgtc accccaccga ggacatccac aggttctgat
     1561 cattgatcgt tgttcttgct ggccgttcgg aacctgcttc gcgccgttga actcgcagaa
     1621 caaaagttcg gtcgacacga gcgagagatc cgagccgcaa aggacgcgtt cctgaacgag
     1681 gtg
//
LOCUS       DASVOR010001428         1691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_44058,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001428 DASVOR010000000
VERSION     DASVOR010001428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1691
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44058"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VF516_27090"
     CDS             <1..1140
                     /locus_tag="VF516_27090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8111437.1"
                     /translation="RGHGALVRSAAFSPDGRRIVSTSYDKTVRVWNADGGEPLVLRGH
                     DVEVNTAAFSPDDRRIVSASKDRTVRVWNADGRGEALVLRGHDASVWSAAFSPDGRRI
                     VSASADRTIRVWNADRGGALLVLRGHDDLVWSATFSSDGRRIVSASADQTVRVWNADG
                     RGEPLVLRGHSDEVRSASFSPDGRYIVSASFDGTVRVWSADGQGEPVILRGHSDGVQS
                     AEFSPDGRYVVSASFDGTVRVWNADGHGDPLVLRGHHDWVLWAAFSPDGRNIVSASKD
                     KTIRIWRADGTGEPVILVGHDLPVEQARFSRDGRRIVSASRDSTVRVWHDLAPATLDD
                     PRLWTATAYCLPVERRIKLLSMSEEQAARDRQRCLDHVERAHQQP"
     gene            complement(1260..1553)
                     /locus_tag="VF516_27095"
     CDS             complement(1260..1553)
                     /locus_tag="VF516_27095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111438.1"
                     /translation="MVGDRTDGPQSETTLDRPTRCQPYEACWLLRPLLGMFVVALALV
                     AVSHVASFFVWALGSVATIAVSRWYKAQFERSHPSAALPSATTRPRRALPPST"
BASE COUNT          253 a          572 c          615 g          251 t
ORIGIN      
        1 cgcggccacg gcgctttggt caggtcggcg gcgttcagcc cggacggtcg gcgcatcgtg
       61 tccacgtctt atgacaagac ggtgcgggtg tggaacgccg atggcggcga gcccctggtc
      121 ctgcgcggcc atgacgtcga ggtcaacacg gcggcattca gcccggacga ccgtcgcatc
      181 gtgtccgcgt cgaaggacag gacggtgcgg gtgtggaacg ccgatggccg tggcgaggcc
      241 ctggtcctgc gcggccacga cgcctcggtc tggtcggcgg cgttcagccc ggacggtcgc
      301 cgcatcgtgt ccgcgtcggc agacaggacg atacgggtgt ggaacgccga tcgaggtggc
      361 gcgcttctgg ttctgcgcgg ccatgacgat ctggtctggt cggcgacgtt cagctcggac
      421 ggccggcgca tcgtgtccgc gtcggcagac cagacggtgc gggtgtggaa cgcagatggg
      481 cgcggcgagc ccctcgtcct gcgcggtcac agcgatgagg tccgctcggc gtcgttcagc
      541 ccggatggcc ggtacatcgt ctccgcgtct tttgacggga ccgtgcgggt gtggagtgcc
      601 gatggacagg gcgagcccgt catcctgcgc ggccacagcg acggggtcca gtcggcagaa
      661 ttcagcccgg atggccggta cgttgtctcc gcgtcttttg acgggaccgt gcgggtatgg
      721 aatgcagacg ggcacggcga ccccctggtc ctgcgcggtc accacgactg ggtcttgtgg
      781 gcagcattca gcccggacgg ccgaaacatc gtctccgcct ccaaggacaa gaccatccgc
      841 atctggcgcg ccgacggcac gggcgagccc gtcattctgg tcgggcacga cctcccggtc
      901 gagcaggcgc gcttcagtcg ggacggccgg cgcatcgtgt ccgcgtcgcg cgatagcacc
      961 gtacgcgtgt ggcatgacct ggccccggcc accctcgacg acccgcgtct gtggacggcc
     1021 actgcctatt gcttgcccgt cgagcgccgc atcaagctcc tcagcatgtc cgaggaacaa
     1081 gccgcgcgcg accgccagcg ttgcctggac cacgtcgagc gcgcccacca gcagccgtga
     1141 cgccgattcc cgatcgcagt acgagcggca acgagattgc cggctcgatc gcacctgatc
     1201 gacacacgac atgccggcgc cgcgatggcc gcggccggcg gcgtggtgga tgagccgggt
     1261 taggtcgatg gtggcagcgc gcggcggggc cgcgtcgtgg cgctcggcag cgctgcgctc
     1321 ggatgggagc gttcgaactg cgccttgtac catcgactga cggcgatcgt cgccacgctg
     1381 ccgagcgccc acacgaagaa cgaggcgacg tgggagacgg cgacgagcgc gagcgcgaca
     1441 acgaacatgc cgagcagcgg gcgcagcagc cagcacgcct cgtagggctg gcagcgcgtg
     1501 gggcggtcga gcgtggtctc gctctgcggt ccatccgttc gatcgccgac catgctccca
     1561 gctttgcaca cgcggcactc gagcgcaccg gacatcacgc gcgcgacagc ggcagctcca
     1621 gccagaacgc ggtgatgccg cccgcgcagt cgacgccgac cgcgccgccg tggccgagcg
     1681 cgaccgagcg g
//
LOCUS       DASVOR010001429        13393 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_335762,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001429 DASVOR010000000
VERSION     DASVOR010001429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13393
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335762"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..697
                     /locus_tag="VF516_27100"
     CDS             <1..697
                     /locus_tag="VF516_27100"
                     /inference="COORDINATES: protein motif:HMM:NF013457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015485 - cholesterol binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiol-activated cytolysin family protein"
                     /protein_id="HEX8111439.1"
                     /translation="DFAYSGGVDLKGDVSVTYKDILSQSKITAFILGGDAGSASQTID
                     SYDALISFIKSGGNYSRQSPGAPIAYKLSYLKDNSPARMSFTTNYTLRDCARVSQQVV
                     VTLNSIYCKSADDGIGDSTLEVYGQITAQGSGPVQSLFAKDSGQTVSISQGQTFGSPM
                     IKQVVISVMPKVGQTITLHAHLFDKDTLSGDDDLGDEISVNPFDTGWRKDVTIQLAID
                     KNNLQVNLSLAPI"
     gene            1003..2652
                     /locus_tag="VF516_27105"
     CDS             1003..2652
                     /locus_tag="VF516_27105"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8111440.1"
                     /translation="MVEGAQIGAYRVLRPIGEGGMGSVWLAEHIALGRRAALKMLRAE
                     LSNRPDIVARFFNEARAATSIADPGIVQIFDFGRHVDGSAYIVMELLDGEPLDRRLRR
                     EGALTVVDGLRVLRQVASTLDAAHARGIVHRDLKPENIFIVRDPEVPGGERPKVLDFG
                     IAKLAGDHVGVKTQTAAVMGTPAFMSPEQCRGAGHVDQRSDVYALGCVLFTLLVGRPP
                     FQADGTGDIIAMHLREPAPAPSQLRPGIPPEVDQLVLRCLAKNAAERFPTAGDLARAI
                     GGLLGSSPEISPARIGGYGSVTAPTTLSGTAGARAPGARAKRSQVGLSAAIAGAGVAS
                     VVAAMVAIRGGDRREAPEAPLPTHAVVPPLAADPPPPGLAPARQPDTSVSQRTPLDRS
                     AQVAPILQAALARFVAWSHDYPGAPCPDAALLGAPDDPWGHPLAITCTEQPGDQIAGA
                     ISAGPDGKRGTTDDIVSWQLGRDVTDVMRGARWPGMPQRTAAKPGVLAKPRQAALAPA
                     GPDVPAKPGPAEASTKPALTQVPARPAPPVQLDENGLPIAR"
     gene            2662..3645
                     /locus_tag="VF516_27110"
     CDS             2662..3645
                     /locus_tag="VF516_27110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111441.1"
                     /translation="MTRAVALVCIAWSATAFSQAAGAQAEVLFRQGRDLLAAGKVAEA
                     CSAFEESQKLEPAVTTLLNLAGCREKLGQLATAWGLFLDAARQTRSAADAASQQLHDI
                     AAARAQRLEPRVPRLTINVPLPNQLDGLEITRGPDRIGAAMWNQALPIDGGTYTITAQ
                     APGMARWTTQVTVAPENDARTVEIPDLRTSVRDDTKPAARTPPPIDGVRAILPRPAPP
                     HASSNTGPIVVGAGGLALLGSGLGLELWAETKYSAAKSEMRSQPQRDSLYHAANTRRY
                     LAEAFAVSGLAAGGTAVWLYLCDDRRRGRAPIDVNVRIVPAAGGLSLSGRF"
     gene            3691..5622
                     /locus_tag="VF516_27115"
     CDS             3691..5622
                     /locus_tag="VF516_27115"
                     /inference="COORDINATES: protein motif:HMM:NF013595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HEX8111442.1"
                     /translation="MRKTMRSPWFVPCLIVLAAASCGFPRLAELADAGNGSEAVFGLE
                     LLAGDIGGLGNEDGTGAAARFNNPAGVAVDSSGNVYIADLGNHTIRKVTAAGVVTTLA
                     GTAGMAGSMDGTAAAARFFNPFGVAVDSFGNVYVADGGNHIIRKVTAAGGVTTLAGTV
                     GMNGSVDDTGAAARFYTPFGVAVDSSGNVYVADLGNGSIRKVTAAGVVTTLAGTAGMD
                     GSVDGTGAAARFSLPSGVAVDTAGNVYVADFGNHTIRKITAVGVVTTLAGTAGMVGSV
                     DGTGTAARFSHPSSVAVDSAGNVYVADNDNHTIRKVTPAGGVTTLAGTAGIVGSADGA
                     GDAARFFRPAGVAVDRAGNVYIADSDGDTIRKVTAAGIVTTTLAGAASAFGSADSTGD
                     AARFFSPAGVAFDSSGNVYVADFGNQTIRKITAAGVVTTLAGTAGMRGSTDGIGAAAL
                     FFDPVGVAVDRAGNVYVADNGNHTIRKITAAGVVTTLAGTAGMDGSVDGTGAAARFFR
                     PSDVAVDSSGNVYVADNGNHTIRKVTPAGVVMTLAGSAGTSGSSDGTGAAARFFNPAS
                     VAIDSTGNVYVTDYSNSTIRKVTPAGTTTTIAGVAGVVGIVLGTIPRFASPISLAIAG
                     DSIIVSDTNAILLLRHGAQ"
     gene            complement(5877..7268)
                     /locus_tag="VF516_27120"
     CDS             complement(5877..7268)
                     /locus_tag="VF516_27120"
                     /inference="COORDINATES: protein motif:HMM:NF024487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HEX8111443.1"
                     /translation="MSHAKSSGPDFGKLNGELTQQVVDELDRAGQRAAVIAEAPPGAG
                     KSTLTASVAKALIDRDQKLRLAIVAQTNEQADDLVVSLTARFRGLKVTRMVGGDGGSS
                     EMKDLANAGSVVISTRCDGADALSTRIVVSTARKWQYERSRMQDSGTASRYPIALIDE
                     AYQMRSDMLLGIAGLYETLFCVGDPGQLDPFTVVDDSLWKGLPYSPSRTALGTLRAFH
                     PSLVPIRLPTSWRLPPSAAGIVSQAFYPFGSFSAGTLEGDRALSLGARRKAAGSAIDT
                     DAVLERAAGTGWGYVELPEKFTSRTDVEIASTIAALVVQLVDRGATVVDERDRAGRAL
                     TLERIAVITTYNDQVHAVRYALSRAGCNPDDLVVSTANRIQGREYDVTLVWHPLAGRR
                     DATAFHLEAGRMCVMLSRHRHACIVVNRAGALRLLEEFPDADPMYLDEPEKFPDGWEA
                     NHVVLRHLATCKV"
     gene            complement(7265..8827)
                     /locus_tag="VF516_27125"
     CDS             complement(7265..8827)
                     /locus_tag="VF516_27125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111444.1"
                     /translation="MSLVQLLLAAEAAAQWKAVRRTAYLHRHVTKRPLVIAVFNLSGE
                     AAAPLGFCYGTDRGKPKVVIAAEPRNRESRFAAINEVCKDLVEHITPYLKLVKIEVRR
                     GGDMDSLKVARDAPQLAVPNRATRDFLGPLLGRSLRYLGLGDTHEVPEQTVWAGAHLS
                     WLGEQSRMPGQSVFLAATELLTRHFVTGQSDLEDENLASLLAWIDNPPGAGRAAIDAA
                     EDVAFGPVPGPPWEATLEEPVRAWSACTRAGDTAGAARAEAQVREIVTARLLPAYDAT
                     HRALDIARAIRRAPSVAERWQADVNDWSAYARWAEHAIPRFTKRHGAIHAATMLERWS
                     RALDQLEFEEAIDDPMIMAELETNGMCVVGEVTKVDLEHKEVKPGNTRATQVPMVTLK
                     LDGDTRLLEGTRVLWCGDSKASGEIRVLAEGHAIVAVVDGHKYGERVPAQGSIAMFAP
                     ISTFGGRSPRDPESVPWTHRRADLETGDEAPATIPADVADDGVPDLPIDELANAPPVG
                     AVTPDDVPGVLQ"
     gene            complement(8824..10065)
                     /locus_tag="VF516_27130"
     CDS             complement(8824..10065)
                     /locus_tag="VF516_27130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111445.1"
                     /translation="MKMDLRSPRMAGILGVLVPRSRGNTLDVIRGSLEPARASVRTVA
                     ALTDNPGCTRRRVIDAARVRAYELAEALGYPVTRGQSPFAITSGNRFEQRLKGGSDYK
                     LLVEVLRPFVELPDTGLKVVDLGHAKRIRLGQAWIEARAKQTDEVLAQIVRGDSEAPH
                     IVDHPVIVFDLAGTPVHLEPDALAFRVGKQLQLVEIKSYAIIDGQADPAKVSATGGQS
                     AVYVLALRAALARLGFDPEIVRWSVILVAPKNFGRAPVAHEVPLRKKAMALERVLRNV
                     PRTDALLAELPEDFTLDVDPDRALSPAARRTRLQQAISQLEMRYVPECFSSCDMARCC
                     REQARAEDNPARIGRAAHDTLAGVETLADALRLATRGPRSDEHHLADVAEALQTAHRA
                     IERARALAPAACKLVPPKDKR"
     gene            10342..10584
                     /locus_tag="VF516_27135"
     CDS             10342..10584
                     /locus_tag="VF516_27135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111446.1"
                     /translation="MQIDVLTSGIWQMNSTILSRRGACVVVDPAYFIDDARAYRATLH
                     RLLEVRRTSKPIARYRTRSRTRPASMACSSMSADLR"
     gene            10860..11636
                     /locus_tag="VF516_27140"
     CDS             10860..11636
                     /locus_tag="VF516_27140"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8111447.1"
                     /translation="MQIDVLTSGIWQTNSTILSRRGACVVVDPAYFPRELDAIAARVR
                     EIGHAAAVVFTHGHWDHVMGHAALPGAPVHVSRVLAESIASGDPRAAKYPDDARDFDA
                     RWYVPRPHGYRWPTGLRGVAEAERAEIGGLALRTLELPGHSPDGLGLVADDALLVGDY
                     LSPCEIPFIDDARAYRATLHRLLEVLTEVRQVIPGHGPRLTAKEATAIARADLDYLDK
                     LLATRDLAAALAVPLPRAADVPGMDGHHRDNCAAAMRALR"
     gene            11684..12460
                     /locus_tag="VF516_27145"
     CDS             11684..12460
                     /locus_tag="VF516_27145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111448.1"
                     /translation="MEPLSSGCGGAGKFTQVRSSNPQVATFTLTDTSTASSTIDMVTG
                     TAGTTDLELLDDHGTVVDSVTIEVEPIAALALAATSARVVAGGSYSVKVTLADAAGRT
                     LGGGMGRIHAAASSGVTTSQGDTFTGTVLELTTPTAGSSTVTVTAEGASSTLALQVVD
                     LSAITAIDVGTGDYTPTYNPNNQVEVVWSTPSTAAGPAYGAKCNWQIGDPSVTLVANG
                     AESDLGRSPFDNSQFKLAKPGRFDVVCTIGTAQLTVTLRR"
     gene            12601..>13393
                     /locus_tag="VF516_27150"
     CDS             12601..>13393
                     /locus_tag="VF516_27150"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8111449.1"
                     /translation="MAGARQEPTLPSVFLSYARADDEDGFVREVYDTLRNRGFTVWWD
                     QVSMPSRALTFLPEIAEAIDASDRLLLVLSPTAVQSEYVRAEWQYALVKDKVVTPVLR
                     RGGFELLPSELEATSCGDARASREKDKALEAIVRLASYPPPPLASAASVPVPPPCYRP
                     RSDPFARVAGRLGLEEPKRLAVPEEERTVLVHGMGGVGKSVLAAAVARSAMIRRVFSD
                     GVIWVAVGRDAQPLAVLQQIALRLGDDLAKYATEAAARQQLAVRLD"
BASE COUNT         2069 a         4647 c         4595 g         2082 t
ORIGIN      
        1 cgacttcgcg tactccggcg gggtcgacct caagggcgac gtctcggtca cctacaagga
       61 catcctctcg cagagcaaga tcaccgcgtt catcctcggc ggcgacgccg gctcggcgag
      121 ccagaccatc gacagctacg atgcgctgat cagcttcatc aagagcggcg gcaactactc
      181 gcggcagtcg cccggcgcgc cgatcgccta caagctgtcc tatctcaagg acaactcgcc
      241 ggcccggatg tcgttcacca ccaactacac gctccgcgac tgcgcgcgtg tgtcccagca
      301 ggtcgtggtc acgctgaaca gcatctactg caagtcggcc gacgatggga tcggcgactc
      361 gacgctggag gtctacggcc agatcaccgc gcagggcagc ggcccggtcc agagcctgtt
      421 cgccaaggac agcggtcaga ccgtgtcgat cagccaaggc cagacgttcg gcagcccgat
      481 gatcaagcag gtggtgatca gtgtgatgcc caaggtcggg cagacgatca cgctccatgc
      541 ccacctgttt gacaaggaca cgctctccgg ggacgatgac ctcggcgacg agatctcggt
      601 caacccgttc gataccggct ggcgcaagga tgtcaccatt cagttggcca tcgacaagaa
      661 caatcttcag gtcaacctgt cgctcgcgcc gatctgaccc gccgccgcgc gacgcggcgt
      721 gcgggtatgc tgattccgcc gccgtgtgcg gcgatgcgcg cggtgaggtc gtacaccgct
      781 tcagcgtttg agcttcgtca cgttgaccag ctgtcggccg tatctgcgca cacacgctcc
      841 cagatggcga gcgaaagatc cgggcacgcc cactctgagc gacaggatcg acgaatctcg
      901 cttccgggcg caacgttcgt actcgacgat gcgtcgagat acgccacgct gatcggcgta
      961 ctgcgatagc aacccacgct acgagcggga tcgagatgac gcatggttga gggcgcccag
     1021 atcggcgcgt atcgtgtgct gaggccgatc ggcgagggcg ggatgggttc ggtgtggctg
     1081 gccgagcaca tcgcgctcgg ccggcgggcg gcgttgaaga tgctgcgcgc cgagctctcg
     1141 aatcggcctg acatcgtcgc gcggttcttc aacgaggcgc gcgcggcgac ctcgattgcc
     1201 gatcccggga tcgttcagat cttcgacttt ggtcgacacg tcgatggcag cgcctacatc
     1261 gtgatggagt tgctcgatgg cgagccgctc gaccgccggc tcaggcgcga gggcgcgctg
     1321 accgtggtcg acgggctgcg cgtcctgcgc caagtcgcga gcacgctcga cgctgcccac
     1381 gcccgcggca tcgtccatcg cgacctcaag cccgagaaca tcttcatcgt ccgcgatccc
     1441 gaggttcccg gtggcgagcg gcccaaggtg ctcgacttcg gcatcgcaaa actcgcgggc
     1501 gatcatgtgg gcgtcaagac acagaccgcg gcggtgatgg gcacaccggc gttcatgtcg
     1561 ccggagcaat gccgtggtgc cggccacgtg gatcagcggt cggatgtcta tgcgctcgga
     1621 tgcgtcctgt tcacgctgct ggtggggcgg ccaccgtttc aggccgacgg caccggcgac
     1681 atcatcgcga tgcacctgcg cgagccggcg cccgcgccgt cgcagctgcg cccggggatc
     1741 ccgccggagg tcgatcaact cgtactgcgt tgcctcgcca agaacgcggc ggagcgcttc
     1801 ccgaccgcgg gcgatcttgc gcgggcgatc ggagggctgc tcggctcgtc gcccgagatc
     1861 tcacccgcgc gcatcggggg ctacggatcg gtgaccgcgc cgaccacgct gtcgggcact
     1921 gccggcgcgc gcgctccggg agcgcgtgcg aagcgatcgc aggtcggtct gagcgcggcg
     1981 atcgcgggcg ctggcgtcgc cagcgtggtg gcggccatgg tggcgatccg cggcggcgat
     2041 cgacgcgagg ccccggaggc tccgctgccc acacacgccg tggtcccgcc actcgcagcg
     2101 gatccaccgc cgcccgggct cgcgccggct cgacaaccgg atacgtcggt gtcacagcgg
     2161 acgccgctcg atcggagtgc gcaggtcgcg cccatcctcc aggccgcgct cgcgcgcttc
     2221 gtcgcgtggt cgcacgacta cccgggcgcg ccatgtcccg acgccgcgtt gctcggcgcg
     2281 cctgatgatc cgtggggcca tcccctcgcg atcacgtgca cggagcagcc cggcgatcag
     2341 atcgcaggag cgatctcggc cggacccgat gggaagcgcg gtaccaccga cgacattgtg
     2401 tcatggcaac tcggtcgcga cgtcacagat gtcatgcgcg gcgcgcgctg gcccgggatg
     2461 ccacagcgca cggctgcgaa gccgggggtg ctggcgaagc cgcggcaggc agcgctcgcg
     2521 ccagccggtc ccgatgtgcc cgcgaagcca gggcccgccg aggcctccac gaagccggcg
     2581 ctcacccagg tgcccgcaag acccgcgccc ccggtgcagc tcgacgagaa tgggttgccg
     2641 atcgcacggt aagggagagt catgactcga gctgtcgccc tcgtttgcat cgcgtggtcc
     2701 gccactgcgt tctcccaggc cgccggtgct caggcggaag tactgtttcg ccaggggcgt
     2761 gacctcctgg ccgcggggaa ggtcgccgag gcatgcagcg cgttcgagga gagccagaag
     2821 ctggagcccg ccgtgacgac gctcctcaac ctcgccggct gccgcgagaa gctgggccag
     2881 ctcgcgaccg catggggact gttcctcgat gccgcccgcc agacccgttc ggcggccgat
     2941 gccgcaagtc agcagctcca cgacatcgca gcggcgcgcg cgcagcggct cgagccgcga
     3001 gtccccagat tgacgatcaa tgtcccgctg cccaatcaac tcgacggcct cgagatcacg
     3061 cggggcccgg accgcatcgg cgccgcgatg tggaaccagg cgctgccgat cgacggcggc
     3121 acctacacga tcacggcaca ggcgccgggc atggcccggt ggacgaccca ggtcacggtc
     3181 gcgcccgaga acgatgccag gaccgtcgag attcctgact tgcgcacttc ggtgcgcgac
     3241 gacaccaagc cagctgcacg cacaccgcca cctatcgacg gtgtgcgggc gattctccct
     3301 cgacctgccc cgccgcacgc gtcgagcaac accggaccca tcgttgtcgg cgcgggtggg
     3361 ctggcgctgc tgggcagcgg gctcggcctc gagctctggg ccgagacgaa gtattccgcg
     3421 gcgaagtccg agatgaggag tcagccgcag cgcgactcgc tgtatcacgc agccaacacc
     3481 agacgctacc tcgccgaggc gttcgcggtg agcgggctgg ctgcgggcgg gaccgcggtg
     3541 tggctgtacc tgtgcgacga ccgtcgccga gggcgcgccc cgatcgatgt gaacgtgcgc
     3601 atcgtgccgg cggcgggggg cctgtctttg tcgggccggt tctgagggtt gcgaccacac
     3661 gttcgagggt gcccctccga cgaggtgacc atgagaaaga cgatgcgaag tccgtggttc
     3721 gttccctgtc tcatcgtcct ggcagcggcc tcctgtgggt ttccgcggct ggcggagctt
     3781 gcagatgcag gcaacggaag tgaggccgtc ttcggcctcg agctcctcgc aggtgacatc
     3841 ggcggcttag gcaatgagga cggcacgggc gccgcggcgc gcttcaacaa cccagccggc
     3901 gtggcggtcg acagctccgg caacgtctac atcgcggacc ttggcaacca taccatccgc
     3961 aaggtcactg cggcgggggt cgtcacgacg ctggccggca ccgcagggat ggctggcagc
     4021 atggacggca cagccgccgc agcgcgtttt ttcaaccctt tcggcgtggc agtcgacagc
     4081 ttcggcaacg tctacgtcgc cgacggtggc aaccatatta tccgcaaggt cactgcggcg
     4141 ggaggcgtga cgacgctggc gggcacggtg gggatgaacg gcagcgtgga cgacacgggc
     4201 gccgcggcgc gcttctacac gcctttcggc gtggcggtcg acagctccgg caacgtctac
     4261 gtcgccgacc ttggcaacgg tagcatccgc aaggtcactg cagcaggggt cgtgacgacg
     4321 ctggcgggca ccgcggggat ggacggcagc gtggacggca caggcgccgc ggcgcgattc
     4381 tccctccctt ccggcgtggc ggtcgacact gctggcaacg tctacgtcgc cgactttgga
     4441 aaccatacca tccgcaagat caccgcagtg ggggtcgtga cgacgctggc gggcaccgcg
     4501 gggatggttg gcagcgtgga cggcaccggc accgcagcgc gcttctctca cccttccagt
     4561 gtggcggtcg acagtgctgg caacgtctac gtcgccgata atgacaacca taccatccgc
     4621 aaggtcaccc ccgcgggcgg tgtgacgacg ctggcgggca ccgcggggat agttggcagt
     4681 gcggacggtg cgggcgacgc agcgcgcttt ttccgccctg ccggcgtggc ggtcgaccgt
     4741 gctggcaacg tctacatcgc cgacagtgat ggcgatacca tccggaaggt tacggccgcg
     4801 gggatcgtga cgacgacgct ggccggcgcc gcgagtgcgt tcggcagcgc cgacagcacg
     4861 ggcgacgcgg cgcgcttttt cagccctgcc ggcgtggcgt tcgacagctc cggcaacgtc
     4921 tacgtggccg actttggcaa ccaaaccatc cgcaagatca cggctgcggg ggtcgtgacg
     4981 acactggctg gtaccgcggg catgcgtggc agcacggatg gcatcggcgc cgcggcgctg
     5041 tttttcgacc ctgtcggtgt ggcggtcgac cgtgctggca acgtctacgt cgccgacaat
     5101 ggcaaccata ccatccgcaa gatcacggct gcgggagtcg tgacgacgct ggcgggcacc
     5161 gcaggtatgg atggcagcgt ggacggaacg ggagccgcgg cgcgcttttt ccgtccttcc
     5221 gacgtggcgg tcgacagctc cggcaacgtc tacgtcgccg acaatggcaa ccataccatc
     5281 cgcaaggtca cgcccgccgg cgtggtgatg acgctggccg gctctgcagg gacatctggc
     5341 agttcggacg gcacaggcgc cgcggcgcgc ttcttcaacc ctgccagtgt ggcgatcgac
     5401 agcacgggca atgtctatgt caccgactac tctaactcca ccatccgcaa ggtcacgcct
     5461 gccgggacga caacgacgat cgcgggtgtg gccggcgtgg ttggaatcgt cttgggcaca
     5521 atacctcggt ttgcgagtcc gatcagtctg gcaatcgctg gtgactccat catcgtcagc
     5581 gacaccaacg ccattctcct cctgcgtcac ggcgcccagt agcgtccacg atcttccatg
     5641 cagatcgacc cgcggaagat cccaccgcgt cagcagcgcg gaatggggga gcagcttcgg
     5701 cgtgacggcg cgagctggcg atcgctcggc caggcgcgcg actggcctgg catgaaactg
     5761 gcttgtggcg ctcgacctca ggtcccgggc aggcgacgct cgcgtgcccg aggtagtccc
     5821 gaccgcggca cggatgagat gaactcgcgc agtcaccacc tcgatcgctg cgatgactac
     5881 accttgcatg tcgccaggtg gcgcaacacc acgtggtttg cctcccagcc gtcggggaac
     5941 ttctccggct cgtcgagata catcgggtcc gcgtccggga actcctcgag caggcgcagc
     6001 gcgccagccc ggttgaccac gatgcacgcg tggcggtgtc gcgacagcat cacgcacatg
     6061 cgtcccgctt cgaggtggaa ggcggtggca tcccggcggc ccgcgagcgg atgccagacc
     6121 agcgtgacgt cgtactcgcg tccctggatc cggttggcgg tcgagacgac cagatcgtcg
     6181 ggattgcagc cggctcgcga cagcgcgtag cggacggcat ggacctgatc gttgtacgtg
     6241 gtgatgacgg cgatgcgctc gagggtcagc gcacgtccgg cacgatcgcg ttcgtcgacc
     6301 acggtcgcac cgcggtccac cagctgcacc accagcgccg cgatcgtgga ggcgatctcg
     6361 acgtccgtgc gcgacgtgaa cttctcgggt agttcgacgt acccccagcc cgtcccggcg
     6421 gcgcgctcga gcaccgcatc ggtatcgatc gcgctccccg ccgccttgcg gcgcgcgccg
     6481 agggacagcg cacgatcacc ctcgagggtg cccgcgctga aggagccgaa tggatagaac
     6541 gcctgcgaca cgatcccggc ggcgctcggt ggcagcctcc aggaggtcgg cagccggatc
     6601 ggcacgaggc tcggatggaa cgcgcgcagc gtaccgaggg ccgtccgcga cggcgagtag
     6661 ggcaggccct tccagagcga gtcgtcgacg accgtgaatg gatcgagctg cccggggtca
     6721 ccgacgcaga acagcgtctc gtagagtccc gcgatgccga gcagcatgtc cgagcgcatc
     6781 tggtacgcct cgtcgatcag cgcgatcgga tagcgcgagg cagtcccgct gtcctgcatc
     6841 cgtgaccgct cgtactgcca ctttcgcgcc gtcgagacga cgatgcgcgt ggacagcgcg
     6901 tccgcgccat cgcatctcgt cgagatgacg acggagcccg cgttcgcaag gtccttcatc
     6961 tccgaggatc cgccgtcacc tcccaccatc ctggtgacct tcaacccgcg gaacctcgcg
     7021 gtgagcgaca cgaccaggtc gtcggcctgt tcgttggtct gggccacgat cgcgagccgg
     7081 agcttctgat ctcgatcgat cagcgccttc gcgacggacg ccgtgagcgt ggacttcccc
     7141 gctccaggcg gcgcctccgc gatgacggcc gcgcgctggc cggcgcggtc gagctcgtcg
     7201 acgacctgct gcgtgagctc tccgttgagc ttcccgaagt ccgggccgga cgactttgca
     7261 tggctcactg gagcactccg ggcacgtcgt ccggcgtcac cgctccgacg ggcggcgcgt
     7321 tcgccagctc gtcgatcggc aggtccggaa ctccgtcgtc ggcgacgtcc gccgggatgg
     7381 tggccggcgc ttcgtcgccg gtctcgaggt ctgcccggcg gtgggtccac ggcaccgact
     7441 ccggatccct cggcgaccgc ccgccgaacg tggagatcgg cgcaaacatc gcgatcgatc
     7501 cctgcgcggg cacgcgctct ccgtacttgt gcccatccac cactgcgacg atcgcatggc
     7561 cttccgcgag gacgcggatc tcgccggacg ccttgctgtc accgcaccag agtaccctcg
     7621 tgccctcgag caggcgcgtg tccccgtcga gcttcaaggt caccattggc acctgcgtcg
     7681 ctcgcgtgtt ccccggcttc acttccttgt gctcgaggtc gaccttcgtg acttccccga
     7741 cgacgcacat gccgttggtc tcgagctcgg ccatgatcat gggatcgtcg atcgcctcct
     7801 cgaactcgag ctgatccagc gcacggctcc atcgctccag catcgtcgcc gcgtggatcg
     7861 cgccgtgtcg cttcgtgaac cgtggaatgg cgtgttcggc ccagcgcgcg tacgcgctcc
     7921 agtcattcac atccgcctgc catcgttcgg cgaccgacgg ggcccgtcgg atcgcacgcg
     7981 cgatgtcgag ggcacggtgg gtggcgtcgt acgcgggaag cagccgtgcg gtcacgatct
     8041 cgcgcacctg cgcttcggcg cgcgcagctc cggccgtgtc gccggcgcgc gtgcaggccg
     8101 accatgctcg cacgggctct tcgagcgtcg cctcccaggg aggaccgggg acggggccga
     8161 acgcgacgtc ctccgcggca tcgatcgctg cgcgaccggc accgggcggg ttgtcgatcc
     8221 acgcgagcag cgacgcgaga ttctcgtctt cgagatccga ctggccggtg acgaagtgcc
     8281 gcgtcagcag ctccgtcgcg gcgaggaaca ccgactgccc gggcatgcgc gactgctcgc
     8341 cgagccacga caggtgtgcg cccgcccaga cggtctgttc cgggacctcg tgcgtgtcgc
     8401 cgagcccgag gtaccggagc gatcgcccca ggagtgggcc gaggaagtcg cgcgtggcac
     8461 ggttggggac cgcgagctgt ggcgcgtccc gcgcgacctt gagcgagtcc atatcgccgc
     8521 cgcggcgtac ttcgatcttc acgagcttca ggtagggagt gatgtgctcg accaggtcct
     8581 tgcagacctc gttgatggcc gcaaagcgcg actcgcgatt cctcggctcc gcggcgatca
     8641 cgaccttcgg cttgccgcga tcggttccgt agcagaaccc gagcggcgcc gcggcttcgc
     8701 ccgagaggtt gaacacggcg atcacgagcg gccgcttggt cacgtgccgg tgcaggtacg
     8761 cggtgcgccg caccgccttc cactgcgccg ccgcctccgc cgcgagcagg agctgcacga
     8821 ggctcatcgc ttgtccttcg gcgggacgag cttgcacgcg gccggggcca gtgcgcgggc
     8881 gcgctcgatg gccctgtgcg cggtctgcag cgcctcggcg acatccgcga gatggtgctc
     8941 gtcggacctc gggccacgcg tcgcgagtcg cagcgcatcc gcgagcgtct cgacgccggc
     9001 cagcgtatca tgcgcggcgc gcccgattcg tgccggattg tcctcggccc gcgcctgttc
     9061 gcggcagcag cgcgccatgt cacagctcga gaagcactcc ggcacgtagc gcatctcgag
     9121 ctgcgagatc gcctgctgga gccgcgtgcg gcgagccgcg ggcgacagcg cgcgatccgg
     9181 gtcgacatcc agcgtgaagt cctccggaag ctccgcgagc agagcgtccg tgcgtgggac
     9241 gttccggagc acgcgctcga gcgccatcgc cttcttgcgc aggggcacct cgtgcgccac
     9301 cggcgcgcga ccgaagttct tcggcgcaac gaggatgacc gaccagcgca cgatctcggg
     9361 atcgaagccg agccgcgcga gggcagcacg cagcgcgagg acatagaccg ccgactggcc
     9421 gccggtcgcc gataccttcg ccggatccgc ctgtccgtcg atgatcgcgt aggacttgat
     9481 ctcgacgagc tgcagctgct tgccgacccg gaacgcgagc gcgtccggct cgaggtgcac
     9541 cggcgtcccc gccaggtcga agacgatcac gggatgatcg acgatgtgcg gggcctcgct
     9601 gtcgccccgc acgatctgcg cgagcacctc atcggtctgc ttcgcccgcg cctcgatcca
     9661 ggcctgcccg agccggatcc gcttggcgtg cccgaggtcc acgaccttca accccgtgtc
     9721 gggcagctcg acgaacggcc gcagcacctc cacgagcagc ttgtagtcac tgccgccctt
     9781 gagtcgctgc tcgaagcggt tgccggacgt gatcgcgaac ggcgactgtc cacgcgtcac
     9841 cggatacccg agggcttcgg cgagctcgta cgcgcgaacg cgagccgcgt cgatcacgcg
     9901 ccgtcgggtg cagcccgggt tgtccgtcaa cgcggcgacc gtgcgcacac tggcgcgcgc
     9961 aggctcgagc gagccgcgga tgacatcgag cgtgttaccc cgggagcgtg gcacaagtac
    10021 tcctagtata ccagccatcc tcggagacct gaggtccatc ttcatcaatt cgccttctgc
    10081 tgtggtcgcg tcatccgccc cgctggagtc acgggctctc ggcgcgtcat gatggttcgc
    10141 cgcgggcgtg gccggcgtgt ccgggaatcg tcctcggtgg aacaccccgg tttgcgaatc
    10201 tgataagtct ggcgatcgct ggtgactcca tgatcatcag cgacaccaac gccatcctcc
    10261 tcctgcgtca cggcgcccag tagcgtccgc caacggaaga tcgcaccgcg ccgcgaccgc
    10321 gcgtgcggta tgctgcgcgg cgtgcagatc gacgtcctga ccagcggtat ctggcagatg
    10381 aactcgacga tcctgtcgcg gcgtggcgcg tgcgtggtcg tcgatcccgc ctacttcatc
    10441 gacgatgcgc gcgcctatcg cgcgacgctg caccggctgc tcgaggtgcg gcggacttcg
    10501 aagccgatcg cacggtaccg aacacgctcg cgtactcgac cagcgtcgat ggcgtgctcg
    10561 tctatgagcg ctgatctccg ctaaggagtt ccgcgccatc aagaaagagc ctcggaggct
    10621 tcgccaaatt tcaccacacg ccacgggatc tagcggaaca cggcggacct cggcgggata
    10681 cggtcggcct caagtgccga gagagttgca gggcgccgac gacaaacggg tttcggaaca
    10741 cctctctcgg gtgaatggtt tggctttgcc ggtccatccc cggggggggg atgggaaggc
    10801 ccctctggat cgaccttcgg cgacgccacg cggcgccgcg tgcgggtatg ctgcgcggcg
    10861 tgcagatcga cgtcctgacc agcggtatct ggcagaccaa ctcgacgatc ctgtcgcggc
    10921 gcggcgcgtg cgtggtcgtc gatcccgcct acttcccgcg cgagctcgac gcgatcgccg
    10981 cgcgggtccg cgagatcggc cacgccgccg cggtggtgtt cacccacggc cactgggacc
    11041 acgtgatggg ccacgccgcg ctgcccggcg cgccggtcca cgtcagccgc gtgctcgccg
    11101 agtcgatcgc gagcggtgac ccgcgcgccg cgaagtaccc cgacgacgcg cgcgacttcg
    11161 acgcgcggtg gtacgtgccg cggccgcacg gctatcgctg gccgaccggg ctgcgcggcg
    11221 tcgccgaggc cgagcgcgcc gagatcggcg gcctcgcgct gcgcacgctc gagctgccgg
    11281 gccacagccc cgacggcctg gggctggtcg ccgacgacgc gctgctggtc ggcgactacc
    11341 tgtcgccgtg cgagatcccg ttcatcgacg acgcgcgcgc ctatcgcgcg acgctgcacc
    11401 ggctgctcga ggtgctgacc gaggtccgcc aggtcatccc cggccacggg ccgcggctca
    11461 ccgccaagga ggccaccgcg atcgcccgcg ccgacctcga ctacctcgac aagctgctcg
    11521 cgacgcgcga cctcgccgcg gcgctcgccg tgccgctgcc gcgcgccgcc gacgtcccgg
    11581 ggatggacgg ccaccatcgc gacaactgcg cggcggcgat gcgcgcgctg cggtagccgg
    11641 cccaaccacg ttcgccgacg gagggaggca cgtcgcgctg gatgtggagc cgctcagcag
    11701 tggttgcggc ggcgcgggca agttcaccca ggtccggtcg agcaatcccc aggtcgcgac
    11761 gttcacgctg accgacacat ccacggccag ttccacgatc gacatggtca ccggaaccgc
    11821 cggaaccacc gacctcgagc tgctcgatga ccacggcacg gtggtcgaca gcgtcacgat
    11881 cgaggtcgag ccgatcgctg cgctggcgct cgcggcgacg agcgcgcgcg tggtcgccgg
    11941 cggcagctac tcggtcaagg tgaccctggc cgacgcggcc gggcgaacgc tgggcggcgg
    12001 gatggggcgg atccacgcgg ccgcgagcag cggggtcacc acctcccagg gcgacacgtt
    12061 caccggcacg gtgctggagc tcacgacccc cacggccggc agcagcaccg tcacggtcac
    12121 ggcggaaggc gcgagctcga cgctcgcgct ccaggtggtc gacctctcgg cgatcaccgc
    12181 gatcgacgtc ggcaccggcg actacacgcc gacctacaac cccaacaacc aggtcgaggt
    12241 cgtgtggtcg acgccgtcga ccgcggccgg cccggcctac ggcgcgaagt gcaactggca
    12301 gatcggcgat ccgtcggtga cgctggtcgc gaacggcgcc gagagcgacc tcgggcgatc
    12361 gccgttcgac aactcgcagt tcaagctcgc gaagccgggc cgcttcgacg tggtgtgcac
    12421 gatcgggacc gcgcagctca ccgtgacgct gcggcggtag ccgcggctcg atcacagcgc
    12481 tgcacgggaa cgtggcgccg atcgtcgaat cggtgcgcgg tggttgccgt cgtggcaacg
    12541 gcgctcgacg gcggcgctcg ggtttgggat tccgtccggc acacgatatc ctcccatcgc
    12601 atggcgggcg cgcgtcagga accgacgctt ccatcggtgt ttctctcgta cgcgcgggcg
    12661 gacgacgagg acggcttcgt acgcgaggtc tacgacacgc tccggaaccg agggttcacc
    12721 gtgtggtggg accaggtatc gatgccgagc cgcgcgctca cgttcctccc ggaaatcgcg
    12781 gaggcgatcg acgcgtcgga tcggttgctc ctcgtgctca gtccgacggc cgtgcagtcc
    12841 gagtacgttc gtgccgagtg gcagtacgcg ctcgtgaagg acaaggtggt cacgccggtg
    12901 ctgcgccggg gcggcttcga gctgcttccg tccgagctgg aggcaacgag ctgcggggac
    12961 gcgcgcgcgt cacgcgagaa ggacaaggca ctcgaggcca tcgtgcgcct cgccagctac
    13021 cctccacccc cgctcgccag cgcggcgagc gtcccggtgc cgccgccctg ctaccgaccg
    13081 cgatccgatc cgttcgcacg cgttgccggt cggctggggc tcgaggagcc gaagcgactc
    13141 gcggtgccgg aagaggagcg aaccgtcctc gttcacggca tgggcggcgt cgggaagtcc
    13201 gtgcttgcgg ccgcagtcgc acgttccgcg atgatcagac gcgtgttctc cgacggcgtg
    13261 atctgggtcg ctgtggggag ggacgcgcag ccgctcgcgg tgttgcagca gatcgcgctg
    13321 cgcctcgggg acgatctcgc gaagtacgcc acggaggcgg ccgcgcgcca gcaactggcc
    13381 gtgcgtctcg acc
//
LOCUS       DASVOR010001430         8251 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_190265,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001430 DASVOR010000000
VERSION     DASVOR010001430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190265"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..796
                     /locus_tag="VF516_27155"
     CDS             314..796
                     /locus_tag="VF516_27155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111450.1"
                     /translation="MTNMTTAHDDRPPTHNDLAIADLVHRALAAVQDAGRFGDRKVFI
                     ASLWTQMLGIEAQTGGTLTDSATIEHFKAWLLRTRLLTRDGTGDGARLVVMCRADLVA
                     AMDPARVAASETVTDGAAFHFVLDSAIARQEYAPRVQSQAQATVPSRVGKVGASRRAA
                     "
     gene            1939..2097
                     /locus_tag="VF516_27160"
     CDS             1939..2097
                     /locus_tag="VF516_27160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111451.1"
                     /translation="MVMIALLPENSADIDWQISTSKRRQAAAREDKTARHDMCFRHRG
                     DTCRACSL"
     gene            2171..3331
                     /locus_tag="VF516_27165"
     CDS             2171..3331
                     /locus_tag="VF516_27165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111452.1"
                     /translation="MIWKHPFRTAVIGAVAALATAAPTGCPPPPPGPTDHDHVESCAI
                     ELDDHTVVDDSNVDMMTFVVGLNHSFPDTTVGVDAAGVEELADIIVSAWPHPEQVVII
                     SSSFFEDCKPTNGACLKAALEKRYGQPLELGEVINNVGEPHRYGTRGVITGSRWHQDR
                     IERVVAPANTEEGVAKVLLRDTVTGLPVTVYAVHTSSGELEVQQLALAEKAQFPAGAM
                     PPIIAGDFNMTEAGNPNPAKFYSENFDWVNRNVMCPGKLFSLQNGNVMQAVLGKTCTN
                     DSAYSYEKATGFLKRVRLSYSVDRQGRLAVRGDHRGADWNGILPNHTAHNVLALGLHL
                     TKQTPSPCFHPPPRCDEIACQEGCESKGWCEGLCVSDACRCRLRPNGDGTCP"
     gene            3543..3974
                     /locus_tag="VF516_27170"
     CDS             3543..3974
                     /locus_tag="VF516_27170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111453.1"
                     /translation="MVMRIARLQDHERNEAFTRLQESIKDLPSGEAQRVLSEIVDRDL
                     INEADHETWFGISKVLTHDEAQASSSLSGACMRAIFDAALTRARSARGRELSFWSTLI
                     DVAESAAVHIENRHRRRSAPEPGCGGERIHESQLTAASWWR"
     gene            4418..5401
                     /locus_tag="VF516_27175"
     CDS             4418..5401
                     /locus_tag="VF516_27175"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8111454.1"
                     /translation="MFDAMLAAEYDPEKRPDGWLQNILRATEQFFDRGQGAIGWLLRR
                     DQQLMPMAPTAINGGERFIETATRIGAGLGARSLSASVAPSRPSLETASEALDLGPDL
                     LHEPAFREHAHPLGIKDFLAFKATSPCGKLSCTIGTPLARIERTSSYDKTPWRLLAKH
                     MSFGLGVLHRSDNQPLELSDAANVFAPDGRVLHLATTAGSDCLASLRRAVLARERARL
                     TRTDPGEAVELLDGALHRYALIDHFETDGKRFVIACAPCDAASPVDKLSPRLRQIANL
                     LVTTGKSRKELAEQLGISIHTLDTEVKALYRRLNVRSRSELVTCARLQDRR"
     gene            6417..7304
                     /locus_tag="VF516_27180"
     CDS             6417..7304
                     /locus_tag="VF516_27180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111455.1"
                     /translation="MSKARTDPQLREALRADWKTAFRKEGLAIPRGELLVVEEPVEGA
                     VLVLPRAVSTVSDPRSDGACGSPGSSSDKATAGPRARESLRPSPQPEPRDPKPRPPTK
                     EVVTTLRKLQFKRWNGNPFGPGCKNDGVLSFTIPSGAVLHSITVGEGDCGPVGADLPV
                     GRYVDSHVALGIRAGGVALNYRPVSGRSSGNASVGYHWWFNQWGHVAFTMCVNVRHKP
                     TVSVASESYFDHVNMDDGLKSYRINTPLCLNKLGVTVSGNTDGSAVKVRPDVSVKTCE
                     NGAIWWFGCKNDRGDVISF"
     gene            complement(7357..7590)
                     /locus_tag="VF516_27185"
     CDS             complement(7357..7590)
                     /locus_tag="VF516_27185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111456.1"
                     /translation="MYAPGDRRFVTSAARAELARVHGRVVGTACAELARVHAAADVAP
                     PAKRYALVVCFLVEAHKTILDHVVEMHRVYMIS"
     gene            complement(7765..>8251)
                     /locus_tag="VF516_27190"
     CDS             complement(7765..>8251)
                     /locus_tag="VF516_27190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111457.1"
                     /translation="EREAEQKERATGHGSANAAPPDAAPGEQGKWRWGIVTEYTWDDV
                     CKVVASGDWLGAIGLLGTALFVDRAIHQQASAAELATLDAAVAENQRERAITFIENVA
                     GAIGAHFGLDRNEVAARAGAAARAAKSPTARPAAAPKRKKRRKRVPVAVPVVPVRVMA
                     R"
BASE COUNT         1521 a         2672 c         2668 g         1390 t
ORIGIN      
        1 ggaacatccg ttgaccagcg gatcgcacat attaccattc tcggccgatg caactgcggt
       61 cagcgtccca ccgcacgcgt cacccgcgat ccatcgcgca gcatgagcgg cgtgctcgcc
      121 accgaccgat ctcgcccgcg gcttcgcccg cctcaagctc ggctcgcaca tcccgaccgc
      181 tgccgagaca ctcggacaca cagatgttgc cgcgcgacaa cacatctcct catgatgtat
      241 aatctgattg tcattggtct agatagatag ccaattcata acactggcac ataatccgca
      301 ttggatgcag gacatgacca acatgaccac cgcccacgac gaccgacccc cgacccacaa
      361 cgacctcgcg atcgccgacc tcgtgcaccg cgctctcgcg gcggttcagg acgctggccg
      421 gttcggcgac cgcaaggtgt tcatcgcgtc gctctggacc cagatgctcg gcatcgaggc
      481 ccagaccggc ggcaccctga ccgacagcgc gacgatcgag cacttcaagg cgtggctcct
      541 ccgtactcgg ttgctcaccc gcgacggcac cggggacggt gcacggctgg tcgtgatgtg
      601 ccgcgccgat ctcgtggctg cgatggatcc cgcgcgcgtc gccgcgagcg agaccgtcac
      661 tgacggagcg gccttccact tcgtgctcga ttcggccatc gcgcgccagg agtacgcgcc
      721 ccgggtccag tcgcaggcgc aggcgacggt gcccagcagg gtaggcaagg tgggcgcgtc
      781 gcgccgggcc gcgtagcggc gtgtcacgcc gcgctggcgg gcagcggcgc cgcctgcgcg
      841 gatccggacg tctccgacgc tgacggcgtg gcggccggca tgggcagcac ctcgacgttg
      901 gacgtcgcca ccgcggggcc gtgtgtgccc gccatcgcac gccgttggcg cgcacgctcc
      961 tgtcgctcgc ggcgccgcgc ggcgcgctcg tcccgccgcc gctgcacgcg atcgcgccga
     1021 gattctctcg gctgcctggc tcgctcggac catcccgagc tcaccaaacc cgtcgcggtg
     1081 gatgccgggg ttgtcggcac gctcgcggcg aacccgaccg ctgccgagct cgccgagctc
     1141 ggcacagagg ctgcgaccgg cgcggccgac gccaccggga tcggttcggc aggggccggg
     1201 atcgcgaccg gcactgcgct cgcgaccata gcatcacgaa ccggcggctc ggccactgcc
     1261 acggtgacag cgatcggcgc tgccggcgct gcggtgcttc gcttcgccgc gtgcgccgcg
     1321 gccggtaccg cgcgattggc cctggccgag ctcggcgcga tcggcgctgc agccacgaga
     1381 ccgggagcca gggcgcgagg gcgggagcag cgaggccacg agatcgggag ccagagcgcg
     1441 agaccggccg caagatctgc tactacgact ggcccggttt tcaggtgtcc ctcaccggtc
     1501 ggttcgtgtg tcccccgagg gcggcggccg gccctggcgg cgatcggggc gccgcgatct
     1561 gcgatcacct gcctgcgacc gtgccggatc agcggttgcg aaatgtgcct tgattaccgc
     1621 atattataaa tgtcggttga cgtaactcat attctgcaag agaattcaga gtgggacaat
     1681 acgacaaata accgcacaga ccccgcgtcg cgagaaagcc gcgaaggcgc ggcgcgcagc
     1741 gcctcgccgc gagggcatcc agccccggcg tcagcccgcg tcgtcggttc cctcgtccat
     1801 cacctcggcg atcgagctcg ctacgatagc ccgctcgtgc cagcgctcca gccgcgcgag
     1861 atcccggaac cagctgccga acgccgtacc caactttggg tactcgcccg cacaggctag
     1921 ccgacataac ttgcgaccat ggtgatgatc gcgctgctcc cggagaatag cgccgacatc
     1981 gattggcaga tctcaacatc caagcgacgg caagcagccg cccgcgaaga caagacggca
     2041 cggcacgaca tgtgctttcg ccatcgtggc gatacttgtc gagcgtgctc gctgtgacac
     2101 gtgacgtcgt tgcagaagcg agacgcggcg cgacggtgcg ctgccccatg agagaaagag
     2161 gaagtgagtg atgatctgga aacatccgtt cagaaccgcc gtgatcggtg cagtggcagc
     2221 cctggcgacg gcagcaccaa ccgggtgccc accgccgcca cctggcccca ccgatcacga
     2281 ccacgtggaa tcctgtgcga tcgagctcga cgatcacacc gtggtggatg acagcaacgt
     2341 cgacatgatg acgttcgtgg tcgggctcaa ccacagcttt ccggatacca ccgtcggtgt
     2401 cgatgccgcc ggcgtggagg agctggcaga tatcatcgtg tcagcgtggc cgcaccccga
     2461 acaggttgtc atcatcagct cgtcattctt cgaggactgc aagcccacca acggggcatg
     2521 cctgaaggcg gcgctggaga agcgatacgg ccaaccgctg gagcttggag aggtgatcaa
     2581 caatgtcggc gaacctcacc ggtacggaac gcgcggcgtc atcaccggat cccgatggca
     2641 tcaagacaga atcgaacgcg tggtggcgcc ggccaataca gaagaaggcg tcgcaaaagt
     2701 cttgctccgc gacacggtga ccggcctgcc ggtcaccgtc tacgcggtcc ataccagcag
     2761 tggagagctc gaggtacagc agctcgcgct cgccgagaag gcgcagttcc cggcgggtgc
     2821 catgcctccc atcatcgcag gtgacttcaa catgaccgaa gcgggcaatc ccaacccagc
     2881 aaaattctac agcgagaact tcgattgggt gaatcgcaat gtgatgtgcc cgggcaagct
     2941 attcagcctg cagaacggga atgtgatgca ggcggtactc ggcaagacct gcacgaacga
     3001 cagcgcatac tcgtacgaga aggcaactgg gttcctgaaa cgtgtacggc tgtcttattc
     3061 cgtcgacagg caaggtcgac ttgcggtgcg cggagatcac aggggtgccg actggaatgg
     3121 gatcctgccg aatcacaccg cccacaacgt gctggctctt gggttgcacc tgacgaagca
     3181 gaccccttcg ccttgctttc atcccccacc gcgctgcgac gagattgcgt gccaggaggg
     3241 gtgcgaaagc aagggctggt gcgaggggct gtgtgtgagc gacgcgtgcc gctgccggtt
     3301 gcgccccaac ggcgatggaa cctgtccctg atccagccgg gtcgaccgcg accgcgcgtg
     3361 cgaggaaggc tcacgcggcc ccacagattc gccggcgtgc gtgccggtcc cggcgctgat
     3421 gtggcgcggg cgggatcgct cggtcgcggc gagggcgcgc agcggcgcgg tggagatcgt
     3481 tcgtccgcag cggtaccccg acgtgccgag cgctgggcat gatctgcccg gggaaagtgg
     3541 acatggtgat gcggatcgcg cgccttcagg atcacgagcg caacgaggcc tttacgcgct
     3601 tgcaagagtc catcaaggat ctcccgagcg gcgaggccca gcgggtcttg tccgagatcg
     3661 tcgatcgcga cctgatcaac gaggcagacc atgaaacctg gttcgggatc tccaaggtgc
     3721 tcacgcacga cgaagcgcaa gcgtcttcgt cgctgagcgg ggcctgcatg cgtgccatct
     3781 tcgacgccgc cttgacccga gcgaggtccg cgcgcggacg ggagctctcg ttctggtcca
     3841 cgttgattga cgtagccgag agcgccgccg tccatatcga gaatcggcat aggaggcggt
     3901 cagctccgga gccaggttgt ggcggtgagc ggatccacga gagccagctg accgccgcat
     3961 catggtggcg gtgatggatc cggtggcgat ggctgcggtg acgtcggacc gacggcggga
     4021 agtgccgtcg tgcaagacgg gtgacgcggc gatgcgatat cgcaaggcct gtacctccga
     4081 gcctagtgtt cgtgacacca ccccgacggc ggattccacg ctacttcggc gagattcctg
     4141 gagaaatgta cccaggtagt tctcgccgtt tcaatgtcga gtcgaaccgc ttgaatccca
     4201 cgctgcttgt gtcatccttc caagcgatcg aacgatcaag aggatcgtcg cgacgggaga
     4261 acggtggatg atggagagtg ggcgggctgg ctgtgatgcg tcgatgtgtg gcgaacgtac
     4321 gcgcaaccgc atgtgcggcc tcgcggcaat gtgagcgccg ccgatggcaa gcacaacgta
     4381 ttgcgtccgg caacctcgga cgatccggcc agccgtattg ttcgacgcca tgctggctgc
     4441 ggagtatgat ccagaaaagc gaccggacgg ctggttgcaa aatatccttc gcgcaaccga
     4501 gcagttcttc gatcgcgggc aaggtgccat cggctggcta cttcggcgag atcaacaact
     4561 catgccgatg gcacccacag ctatcaatgg tggtgagcga ttcatcgaga ccgccactcg
     4621 catcggtgcc gggctgggcg caaggtcgct gtccgcctcg gtggcgccaa gccggcccag
     4681 cctggagacc gcaagcgaag cgcttgacct tggccccgac ctgctacacg agccggcgtt
     4741 tcgcgaacac gctcatccgc tgggaatcaa ggacttccta gcgttcaagg ccaccagccc
     4801 gtgcggcaag ctcagttgta ccatcggaac gccgctggcg cgtatcgagc gcacctcgtc
     4861 gtatgacaag acgccctgga ggctgctcgc aaagcacatg agctttggac tgggagtcct
     4921 gcaccgttcc gacaaccagc cgctcgagct ctcggatgcg gccaacgtgt ttgctccgga
     4981 cggtcgcgta ctccatctgg ccacgaccgc gggttccgat tgtctcgcga gccttcgccg
     5041 ggccgtgctc gcgcgcgaac gggcgcggct cacacggacc gatccaggcg aagcagtcga
     5101 gcttctcgat ggagcattgc atcgctatgc gttgatcgat cacttcgaga ccgacggtaa
     5161 gcgtttcgtg atcgcctgcg ccccgtgtga cgccgccagt ccggtggaca agctgtcgcc
     5221 gcgcctgcgc cagatcgcca acctgctggt gaccaccggc aagagccgta aagagctggc
     5281 cgagcaactc ggcatatcga tccatacgct cgacaccgag gtcaaagcgt tatatcggcg
     5341 gctcaacgtt cgcagcagat ccgagctcgt gacttgcgct cgcctacagg accgacgctg
     5401 agcggtcggt gatcgagctg acctgaaact tacccatctg cgagggggct cctggacgag
     5461 gtgtatccga ggccgcgggc ggcttccttg agggacgcca ggcggcgacc ctcaaaatgg
     5521 gcctcgtacg ccttctcgcc gatgtcgacg tagtcttggc cgtaccgcag catgcggtag
     5581 ggcctgctac tcgcaaacgc cggtcctgaa cgaaccatag ctgctgtcac caggagatga
     5641 cgtggccgtg ccctggtccg tcgccgtcgg gagcgaagca gtcggacccg gcccagctgc
     5701 gtctccgacg gccgagatca ccgcagttcg ccgatgagct tggcatctcg atccatacat
     5761 tcgataccga agccaaagca ttgtatcgac gttcaacgtt cgcagcaggc ccgagctggc
     5821 ggcgcggatc cgcgactacc caactttggg tactcgctgg caccaggcta ctgatctact
     5881 gttgtggacg tcgcgcaaaa atggcacgcg ctcctggact cgaacacccc gatctggccc
     5941 aatttcatcc attgtcgcta gtttttggtc ttgtcacaat cccggttaat gagagccgac
     6001 atatccaact gagagacaca catgattgca aattttatac atgtcgatga gttcatggca
     6061 acgcactcca acaacatgac ttttctcgcg cacgtcgaat acgtaggcat cttcgttccg
     6121 gcgcccgtgg gatctctgcc ttgataaagg acacgatcat gaatacgcat gatacgagga
     6181 cagcggacgt cgcgggttcc atcgcggcgt cgctgcgatc accagtcgcg gcccaagctg
     6241 cgacggccca ccgcggaacg aatcctggag gacatatggc tggtagaaac cttattgcgg
     6301 tacttctggc agtggcagcc ccgacggtgg cagccccgac agctgcgtgg gcggacgaac
     6361 cagtgccgct aggcgatgca gcagactcgt ccgcgacgag cccctcatcc cgactgttga
     6421 gcaaggcgcg aaccgacccg caattgcgcg aggcgctccg cgccgactgg aagacggcgt
     6481 tccgcaagga agggttggcg attccgcgcg gcgagttact cgtcgtcgaa gagcctgtgg
     6541 aaggagctgt gctggtcctg ccgcgcgcgg tgtcgacagt cagcgatccg cgatcggatg
     6601 gcgcgtgcgg ctcacccggg tcgagcagtg acaaggcgac cgccgggccg cgcgcacgag
     6661 agagtctgcg cccgtcgcca caaccggagc cgcgagatcc caaaccgcga cccccgacca
     6721 aggaggtcgt gaccacgcta cgaaagctcc agttcaagcg ctggaacgga aatccattcg
     6781 ggcccggctg taagaacgat ggggtactca gcttcaccat cccatccggg gcggtgctgc
     6841 actcgattac cgttggcgag ggcgattgcg gtccggttgg cgccgatctt ccggtcgggc
     6901 gatatgtcga ttcccatgtc gcccttggaa tcagggccgg aggggtagca ctcaactatc
     6961 gtccggtatc aggacggagt agtggaaacg cgtcggtcgg ataccactgg tggttcaatc
     7021 agtgggggca tgtcgccttc acgatgtgtg tgaacgtgag gcacaagccc acggtcagtg
     7081 tggccagtga gagctacttc gatcacgtga acatggatga cgggctgaag tcttatcgaa
     7141 tcaatacgcc gctgtgtctt aacaagctgg gtgtcaccgt cagcggaaac accgacggca
     7201 gcgcggtgaa ggtccgcccc gacgtcagcg tcaagacgtg tgaaaatggc gcgatctggt
     7261 ggttcggctg caagaacgac cgaggcgacg tgatctcgtt ctgagccttc gggtcacgtg
     7321 cagcgacaac acgacgacta tctgctaact ccacgatcaa gaaatcatgt acacccggtg
     7381 catctcgacc acgtgatcga ggatggtctt gtgcgcttcg acaaggaagc agacgaccag
     7441 cgcgtaccgc ttcgccggcg gcgcgacgtc ggccgcggca tggacacggg cgagctcggc
     7501 gcacgcggtg ccgacgacgc ggccatggac acgggcgagc tcggcgcgtg cggcgctggt
     7561 gacgaatctc cgatcgcctg gcgcgtacaa cccgggcgct cgagttgctc gcgctggcgg
     7621 ggcgccgcac cagctcggcg cgtccctgcc cgctcgcgca agctgccggc actcgcgctt
     7681 gttgtcgacc cggcgatcgc ggcggcggtc ccccggcgtg gggccggatc ggaagcagcg
     7741 tggcgaggat gtcgagcgag gcgatcagcg agccatgaca cgcaccggga cgacagggac
     7801 cgcgaccggc acgcgcttgc gtcgcttctt ccgcttcggg gcggctgccg ggcgcgccgt
     7861 gggcgatttc gctgcgcgtg ccgccgcacc ggcacgtgct gcgacctcgt tgcggtcgag
     7921 gccgaaatgg gcgccaatgg caccggcaac gttctcgatg aatgtgatcg ctcgctcgcg
     7981 ttgattctcg gccaccgccg cgtccagcgt cgcgagttcg gcagcgctgg cctgctggtg
     8041 gatggcgcga tcgacaaaca gagcggtgcc caggaggccg atcgcaccca gccagtcccc
     8101 agacgccacc accttgcaga cgtcgtccca cgtgtactcg gtgacgatgc cccatctcca
     8161 tttcccttgc tctcccggcg cagcgtccgg gggagcggcg ttcgcggatc cgtgccccgt
     8221 ggcgcgttcc ttctgctccg cttcacgctc c
//
LOCUS       DASVOR010001431         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_72948,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001431 DASVOR010000000
VERSION     DASVOR010001431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72948"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            380..958
                     /locus_tag="VF516_27195"
     CDS             380..958
                     /locus_tag="VF516_27195"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8111458.1"
                     /translation="MSNTNSQVVVGYDFSPSGAAALSRAIALAARAPFHVLHVACIVD
                     RHAQLPGHEHHGRVDFAYTERVQQAVIDVIDQELLAADLGDRIQFYVHVRIGKPADEL
                     LGLARDIGADLIIVGSKGLTGVERIVLGSVSEKVVREARCTVEVARLKTYDHVELSDI
                     VEVAPHRTYVPPHRYTYEDPRLEMRPPEWPLY"
     gene            complement(971..1411)
                     /locus_tag="VF516_27200"
     CDS             complement(971..1411)
                     /locus_tag="VF516_27200"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8111459.1"
                     /translation="MNATEARTTLLAQHEQLRLLLETCTRLARQQRDGEPVTAELDQV
                     LAELRDEFADHNRMETAVIAELLEGPAAWGAQMVDRMFEEHVAEHAAFWELLSGTRDE
                     VAGRIGELAEELDAHMSAEERTFLSPMTLRDDVIRRRTRELVTE"
BASE COUNT          212 a          528 c          555 g          241 t
ORIGIN      
        1 cgacagcgac gacgacttcg tggccaagct gcaccgcgcg ctcgacgtga tccgcgccga
       61 ggtcccggcg tgctgccggg cgaagccggc gggctagcct cgatgccgaa cgcgtagacg
      121 tgggcagcgt agggatcgag cgacgcctct tcacgccccc gtgcgtccat cgaacgaagc
      181 gcgatggtcg ccgggtgccg gcagggttgg acgggtcggt tggtgcggta ccgcggcgac
      241 catcgcgccg attcgtggac agacgttcag atgcgacgtg ggagccgctc catggcggtg
      301 gtgttctcga gtgccggccg cggcggttgc gcgttccggt gcccggatcg ctggcatgtc
      361 ggttgcgatg aatcaccgca tgagcaacac caactctcag gtcgtcgtcg gatacgactt
      421 cagcccctcg ggcgccgcgg cgctgtcgcg cgcgatcgcg ctggccgccc gggccccgtt
      481 ccacgtgctc cacgtcgcgt gcatcgtcga ccgccacgcg cagctgcccg gccacgagca
      541 ccacgggcgg gtcgacttcg cctacaccga gcgcgtgcag caggcggtga tcgacgtgat
      601 cgaccaggag ctgctcgccg cggacctcgg ggaccggatc cagttctacg tccacgtccg
      661 gatcggcaag ccggccgacg agctgctcgg cctggcgcgc gacatcggcg ccgacctgat
      721 catcgtcggc agcaaggggc tgaccggggt cgagcgcatc gtgctgggct cggtctccga
      781 gaaggtggtg cgcgaggccc ggtgcaccgt cgaggtcgcc cggctcaaga cctacgatca
      841 cgtcgagctg agcgacatcg tcgaggtcgc gccgcatcgc acgtacgtgc cgccccatcg
      901 ctacacgtac gaggatcccc ggctcgagat gcggcccccg gagtggccgc tgtactagcg
      961 gtggttccgg ttactcggtc accagctcgc gggtccggcg gcggatcacg tcatcgcgca
     1021 gcgtcatcgg gctgaggaac gtccgctcct cggccgacat gtgcgcgtcg agctcctcgg
     1081 ccagctcgcc gatccgcccg gcgacctcgt cgcgcgtgcc ggacagcagc tcccagaacg
     1141 cggcgtgctc cgcgacgtgc tcctcgaaca tgcggtcgac catctgcgcg ccccaggccg
     1201 cggggccctc gagcagctcg gcgatcaccg cggtctccat ccggttgtgg tcggcgaact
     1261 cgtcgcgcag ctcggcgagc acctggtcga gctcggcggt caccggctcg ccgtcgcgtt
     1321 gctggcgggc cagccgggtg cacgtctcga ggaggaggcg gagctgctcg tgctgcgcaa
     1381 ggagcgtggt acgggcttcg gtggcgttca tgacatgggc tttcgcaaag gtcgggccgg
     1441 gcatcgcgcg cggtttgcac agagttaggt cggggcgccg gtgatttcgc gcagcgattg
     1501 cgccgcgccg ccaggatcgc cgcaggcagt gcggaa
//
LOCUS       DASVOR010001432         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_276811,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001432 DASVOR010000000
VERSION     DASVOR010001432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276811"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..>1686)
                     /gene="ggt"
                     /locus_tag="VF516_27205"
     CDS             complement(135..>1686)
                     /gene="ggt"
                     /locus_tag="VF516_27205"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8111460.1"
                     /translation="KVGRDILAAGGNAVDAAVALAFALAVTHPTAGNIAGGGFAVVRT
                     GPGKAVALDFREVAPAAATADMYLDKDDNPTDRSLHGDLAVGVPGSVAGLWELHHRLG
                     KKPWKAVVAPAIALASGGFEVTEKLHQAIVALTPLLARSPASAALWLPGGVARAAGDR
                     VTNPELAAALGRIAERGPDGFYKGATAAAIVAEMQAGGGIITADDLAKYKPIWRDPLR
                     FSYRGYSVAAMPLPSSGGIVLAMTAGMLGKLELGKLAWYGAEHIHWLGEVWRRAFAAR
                     NELLGDPAYVKDVPVAKLVSRAYLDQLFATITDKATPSRQVTALLEGDHTTNLCTVDA
                     HGMAVALTTTLNTAFGNGVTVAGFFLNNEMDDFASKPGSPNTFGLVQGAANKIEPGKR
                     MLSSMSPTIVEDDRGRLFMLAGAGGGPRIITAVWQTISNVIDFRKPADLAVAAPRIHH
                     QHLPDMISVEAGAIDQPTDEQLRAMGYRLHWGEPHRGFAAITAIVRGATGWDGTSDPR
                     AGGAAIGD"
BASE COUNT          221 a          616 c          613 g          236 t
ORIGIN      
        1 gtgggtcgac gcccggggct cgtcccggcg cggccgcggc accggcagct gcgggtagac
       61 caggaccccg ctctcgttgt caccgctcga ggccatgacg agaagctagc accaaccgcg
      121 cgacgtcacg cgcgtcagtc gccgatcgcc gcgccgccgg cgcgcggatc ggaggtgccg
      181 tcccaccccg tggcgccgcg cacgatcgcc gtgatcgccg cgaacccgcg gtgcggttcg
      241 ccccagtgca agcggtagcc catcgcgcgc agctgctcgt cggtcggctg atcgatcgcc
      301 ccggcctcga ccgagatcat gtcgggcaaa tgctggtggt ggatccgcgg cgcggcgact
      361 gcgagatcgg ccggcttgcg gaagtcgatg acgttggaga tcgtctgcca caccgcggtg
      421 atgatccgcg gcccgccgcc ggcgccggcc agcatgaaca gccggcccct gtcgtcctcg
      481 acgatggtcg gcgacatcga cgacagcatc cgcttgccgg gctcgatctt gttggcggcg
      541 ccctggacca gcccgaacgt gttgggcgat ccgggcttgg acgcgaagtc gtccatctcg
      601 ttgttgagga agaaccccgc gacggtcacg ccgttgccga acgcggtgtt gagcgtggtg
      661 gtcagcgcga ccgccatgcc gtgggcatcg accgtgcaca ggttggtggt gtggtcgccc
      721 tcgagcagcg ccgtgacctg gcgcgacggc gtcgccttgt cggtgatcgt cgcgaacagc
      781 tgatcgaggt acgcccgcga cacgagcttc gccaccggga cgtccttgac gtacgccgga
      841 tcgccgagca gctcgttgcg cgccgcgaac gcgcgccgcc agacctcgcc cagccagtgg
      901 atgtgctcgg cgccgtacca cgccagcttg ccgagctcga gcttgccgag catgccggcg
      961 gtcatcgcga gcacgatgcc gcccgacgac ggcagcggca tcgccgccac cgagtagccg
     1021 cggtaggaga accgcagcgg gtcgcgccag atcggcttgt acttcgcgag gtcgtcggcg
     1081 gtgatgatgc cgccgccggc ctgcatctcg gcgacgatcg ccgccgcggt cgcgcccttg
     1141 tagaacccgt cgggcccgcg ctccgcgatc cggccgagcg ccgcggcgag ctcggggttg
     1201 gtgacccggt cccccgcggc acgcgccacg ccgcccggca gccacagcgc cgcgctggcc
     1261 ggcgacctcg cgagcagcgg cgtgagcgcg acgatcgcct ggtgcagctt ctcggtgacc
     1321 tcgaagccgc ccgaggccag cgcgatcgcg ggcgcgacca ccgccttcca cggcttcttg
     1381 ccgagccggt ggtgcagctc ccacagcccg gcgaccgacc cggggacgcc gaccgcgagg
     1441 tcgccgtgca gcgagcggtc ggtcgggttg tcgtccttgt cgaggtacat gtccgcggtg
     1501 gccgccgccg gggcgacctc gcggaagtcg agcgcgacgg ccttgcccgg cccggtgcgc
     1561 acgacggcga atccgccgcc ggcgatgttg cccgccgtcg ggtgggtcac cgcgagggcg
     1621 aacgcgagcg cgaccgcggc gtcgaccgcg ttgccgccgg cggccaggat gtcgcggccg
     1681 accttg
//
LOCUS       DASVOR010001433         4259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_103019,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001433 DASVOR010000000
VERSION     DASVOR010001433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103019"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..556)
                     /locus_tag="VF516_27210"
     CDS             complement(71..556)
                     /locus_tag="VF516_27210"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX8111461.1"
                     /translation="MTAPIRVAIIGGGCAAMTTAFELSRPEHRGRYRITVYQQGWRLG
                     GKGASGRGPAGRIEEHGLHVWMGWYDNAFRLLRDCYGELAATPGAWPALHWRDVFVPD
                     AYVGVADRARRGGWSRLRACMPPRPGAPGDPIAGGPFTIGNYLRCAVELLIAGLRDLH
                     L"
     gene            complement(553..2994)
                     /locus_tag="VF516_27215"
     CDS             complement(553..2994)
                     /locus_tag="VF516_27215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111462.1"
                     /translation="MAQADRILGPPFRFDGVTARVFPLRAHAHALQRLCDTYLNLDRD
                     QVCFRPSAPYVLLVLLNYGEMSYASETAAHYGWVSQNEVYFGAPLDWRRRRNGRWETG
                     SGVVAPFIFVDQAWSIEIGREVYGWPKMPAEFLRQVNTWASGSPAARENLLTLATETF
                     LTPYASERPSSVPLVEIDRAAPLSIGPSLWQAPLSIDNPWWGPMTLAREAMRGWWPTG
                     LSDAPMYVPLMLQSLRMLYGGPSPAFYTANLKQVRSLEDPTLAAYQAITMAPMQLTSL
                     HRAGLLGESRILLGDPTGGYRIRVQRHPMFPIIETLGLVVSEEESHSDARDVAIRGYT
                     AGATGNTRLERFPEGLTSSDDLEHGVATLAPLFPYWLEGDLMYGCGDTITWRDSQHGG
                     TVWARRAPGRDAGRDAGSDAGSDARRAAVTPTTDDRDDRREEPPHSYDATWGARGIPA
                     PPYYYPQLTMRFVPLKVTSERDLVDAIQALEPPEQIGELAPFMPGGTPFVIMMVVTSE
                     EMGAEVNSAGWWWKRQVSFCVPVTWTPRHPWPASIRKPGPVALLSMATFVDSELAATT
                     AREIGPEVIAANLYAPPDSWLDHDGPRADRRLLWLSTTVLPALDVGAEAKQRVLLELL
                     AVNPAHIAPGARAAMPEVAPPNIPDDGMPICILARRRIRDATDPGTYAYQEWFSQHML
                     SRRLPDCPGWQLSPDPLELRLHQYPDPLDLVRYLTLHVSDSEYTKSQRPGRGWEGATV
                     NVITTGFAWATLDVTQECNTSLAVCSHGTWWDPRTEAPPEPGPVAVSLAAAPQRSNPL
                     EDLLAGLAGHVKTPR"
     gene            complement(3006..4040)
                     /locus_tag="VF516_27220"
     CDS             complement(3006..4040)
                     /locus_tag="VF516_27220"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEX8111463.1"
                     /translation="MAAPPPVVDEVLRDYGALVRTAVREYLPAAEPRRYLYDLVADYP
                     GRGGKMMRPSLCIAVARALGAPLDRLLIAAATSIELMHNAMLIHDDIEDESDRRRGQP
                     TLHSLWGIAMAINAGDALVLTSLRPLLGAAHISPFLALGILEETERMARESAEGQALE
                     LGWRAENVVALEERDYFEMVLKKTCWLATIHPVRLGALIAVAGRVDPTAFVRFGFFLG
                     TAFQIQDDVLNLVGDAAAYGKETGGDLREGKRTLMIIRLLQLATPDERARLIAYLGTA
                     LPARREAEARWIRDRMDHHGCIDYARHVARAMADAALAEYTGLFAPVPPSRDRDFLEG
                     LITWVVERTS"
     gene            complement(4042..>4259)
                     /locus_tag="VF516_27225"
     CDS             complement(4042..>4259)
                     /locus_tag="VF516_27225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111464.1"
                     /translation="LQPGAGGALEVHGLLAPAAAAAPPITDVTVAVAGGTVTVAIGAI
                     DHHPAGTYAGAVLAAGRACGTLTVRLS"
BASE COUNT          584 a         1455 c         1653 g          567 t
ORIGIN      
        1 cgaggctgcg cggctcggcg cgcggcggca tcgccggcgg gccggcgggc gcgccgtgca
       61 ccccgagggt ctagaggtgg aggtcgcgca gcccggcgat cagcagctcg acggcgcagc
      121 gcaggtagtt gccgatcgtg aacgggccgc ccgcgatcgg atcgcccggc gcacccgggc
      181 gcggcggcat gcaggcccgg aggcgcgacc agccgccccg ccgagcgcga tcggcgacgc
      241 cgacgtaggc gtcggggacg aacacgtcgc gccagtgcag cgccggccac gcgccggggg
      301 tggcggcgag ctcgccgtag cagtcgcgga gcaggcggaa cgcgttgtcg taccagccca
      361 tccagacgtg caggccgtgc tcctcgatcc ggccggccgg gccgcggccc gaggcgccct
      421 tgccgccgag ccgccagccc tgctggtaga ccgtgatgcg gtagcggccg cggtgctcgg
      481 ggcgcgacag ctcgaacgcc gtggtcatcg cggcgcagcc accgccgatg atggcgacgc
      541 ggatcggggc cgtcaccggg gcgtcttcac gtggccggcg agtccggcca gcaggtcctc
      601 gagcgggttc gagcgctgtg gcgccgcggc gagcgatacg gcgaccgggc cgggttccgg
      661 cggggcctcc gtcctcggat cccaccacgt gccgtggctg cacaccgcca ggctggtgtt
      721 gcactcctgg gtgacgtcga gcgtcgccca cgcgaacccg gtcgtgatga cgttgaccgt
      781 ggcgccctcc cagccgcggc ccgggcgctg gctcttggtg tactcggaat cggagacgtg
      841 gagggtgaga tagcggacga ggtcgagcgg gtcggggtac tggtggaggc ggagctcgag
      901 cgggtcgggg ctgagctgcc agcccgggca gtccggcaag cggcgtgaga gcatgtgctg
      961 gctgaaccat tcctggtatg cgtaggtccc ggggtcggtc gcgtcgcgga tccggcggcg
     1021 cgcgaggatg catatcggca tcccgtcgtc cgggatgttc ggcggagcta cctcgggcat
     1081 ggcggcgcgc gcaccgggcg cgatgtgggc cgggttgacc gcgagcagct cgagcaggac
     1141 gcgctgcttg gcctcggcac cgacgtcgag cgcgggcagg acggtggtgg acagccacag
     1201 caggcggcgg tcggcgcggg ggccgtcatg gtcgagccac gagtcgggcg gcgcgtagag
     1261 attggccgcg atgacctcgg ggccgatctc gcgcgcggtc gtcgccgcga gctcgctgtc
     1321 gacgaacgtc gccatcgaca gcagggccac cgggcccggc ttgcggatcg acgccggcca
     1381 cgggtgccgc ggggtccagg tcaccggcac acagaacgag acctggcgct tccaccacca
     1441 gcccgcgctg ttgacctccg cgcccatctc ctccgacgtg acgaccatca tgatgacgaa
     1501 cggcgtgccg cctggcatga agggcgcgag ctcaccgatc tgctcgggcg gctccagggc
     1561 ctggatcgcg tccacgaggt cgcgttcgct ggtgaccttg agcggcacga agcgcatcgt
     1621 gagctgcggg tagtagtacg gcggcgccgg gatcccgcgc gcgccccacg tcgcatcgta
     1681 cgaatgaggc ggctcctcgc gccgatcgtc ccgatcgtcc gtcgtggggg tgacggcggc
     1741 gcgtcgcgca tcgctcccgg catcgctccc ggcatcgcgg ccggcatcgc ggccgggagc
     1801 ccgtcgagcc cagacggtgc cgccgtgctg cgagtcgcgc caggtgatgg tgtcgccgca
     1861 gccgtacatg agatcgccct cgagccagta cgggaacagc ggcgcgaggg tggcgacgcc
     1921 gtgctcgaga tcgtcgctgg aggtcaggcc ctcggggaag cgctcgaggc gggtgttgcc
     1981 ggtcgccccc gcggtgtagc cgcggatcgc gacgtcgcgg gcatcggagt gggactcctc
     2041 ctcggagacc accaggccga gggtctcgat gatcgggaac atcggatggc gctggacgcg
     2101 gatccggtag ccgccggtgg gatcgccgag caggatccgg ctctcgccga gcaggccggc
     2161 gcggtggagc gaggtcagct gcatcggcgc catggtgatc gcctggtacg ccgcgagggt
     2221 gggatcctcg aggctgcgca cctgcttcag gttggcggtg tagaacgcgg gcgacggccc
     2281 gccgtacagc atgcgcagcg actggagcat cagcggcacg tacatcgggg cgtcgctgag
     2341 cccggtcggc caccagcccc gcatcgcctc gcgcgccagc gtcatcgggc cccaccacgg
     2401 gttgtcgatc gacagcggcg cctgccacag gctggggccg atgctgagcg gcgccgcgcg
     2461 atcgatctcg accagcggca cgctgctcgg ccgctcgctg gcgtacgggg tcaggaaggt
     2521 ctcggtggcg agggtcagaa ggttctcgcg cgcggccggg ctgccgctgg cccaggtgtt
     2581 gacctggcgc aggaactcgg ccggcatctt gggccagccg tagacctcgc ggccgatctc
     2641 gatcgaccac gcctgatcga cgaagatgaa cggcgcgacc acgcccgagc cggtctccca
     2701 ccggccgttc ctgcgccgcc gccagtcgag cggcgcgccg aagtagacct cgttctgcga
     2761 cacccagccg tagtgcgccg cggtctcgct cgcgtacgac atctcgccgt agttgagcag
     2821 gacgagcagc acgtacggcg cggacggccg gaagcagacc tggtcgcgat ccaggttgag
     2881 gtaggtgtcg cacaggcgct gcagcgcgtg ggcgtgggcg cgcagcggga acacgcgcgc
     2941 ggtgacgccg tcgaagcgga acggaggtcc gaggatgcgg tcagcctggg ccatggcggt
     3001 ctccttcagc tggtgcgctc gacgacccac gtgatcaggc cctcgaggaa gtcgcggtcg
     3061 cgcgacggcg gcaccggcgc gaacagcccg gtgtactcgg cgagcgcggc gtcggccatg
     3121 gcgcgggcga catggcgggc gtagtcgatg cagccgtgat ggtccatccg atcgcggatc
     3181 caccgcgcct cggcctcgcg gcgcgccggc aaggccgtgc cgaggtaggc gatcaaccgc
     3241 gcgcgctcgt cgggcgtcgc gagctggagc agccggatga tcatcagcgt gcgcttgccc
     3301 tcgcgcagat cgccgccggt ctccttgccg taggcggcgg cgtcgccgac caggttgagc
     3361 acgtcgtcct ggatctggaa cgcggtgccg aggaagaacc cgaagcggac gaacgcggtc
     3421 ggatcgaccc ggccggcgac cgcgatcagc gcgcccagcc gcaccgggtg gatcgtggcc
     3481 agccagcagg tcttcttgag caccatctcg aagtagtcgc gctcctcgag ggcgaccacg
     3541 ttctcggcgc gccagccgag ctcgagcgcc tggccctcgg ccgactcgcg ggccatgcgc
     3601 tcggtctcct cgaggatgcc cagcgccagg aacgggctga tgtgcgcggc gccgagcagc
     3661 gggcgcaggc tggtcagcac gagcgcgtcg ccggcgttga tcgccatcgc gatgccccac
     3721 aggctgtgca gcgtgggctg gccgcgccgg cggtcgctct cgtcctcgat gtcgtcgtgg
     3781 atcagcatcg cgttgtgcat cagctcgatc gaggtcgcgg cggcgatcag caggcgatcg
     3841 agcggcgcgc cgagcgcgcg ggcgaccgcg atgcacaggc tcggccgcat catcttgccg
     3901 ccgcggcccg ggtagtcggc gaccagatcg tagaggtagc ggcgcggctc ggcggcgggc
     3961 aggtactcgc gcacggcggt ccggaccagc gcgccgtagt cgcgcaggac ctcgtcgacc
     4021 accgggggcg gcgcggccat gtcaggacag ccggacggtg agggtgccgc acgcgcggcc
     4081 cgcggcgagc acggcgcccg cataggtacc ggcgggatgg tgatcgatcg cgccgatcgc
     4141 caccgtgacc gtccctccgg cgaccgcgac ggtgacgtcg gtgatcggcg gcgccgcggc
     4201 ggcggccggg gcgagcagac cgtggacctc gagggcgcca ccggcgccgg gctgcagat
//
LOCUS       DASVOR010001434         4106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_103022,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001434 DASVOR010000000
VERSION     DASVOR010001434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103022"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(576..1370)
                     /locus_tag="VF516_27230"
     CDS             complement(576..1370)
                     /locus_tag="VF516_27230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111465.1"
                     /translation="MQSRSSRPRFWIALQPSIVRVYKAAGLVALTAILVGLVGFLVVN
                     IFYFFDHTWVRPVVLSPNHQKVVEASNLLADAKTRASRLSGELASLEESVAAHERAIA
                     DDERFLAEIGAAADAPRTAEQWLVRREVEKARLDKDNKVGERAPLGLQIEQQRRLIKD
                     QEAVVNRLAQSPYLKAIENKVTLAFVANQNLRNIKLGTPLYGCIWGLVWCHKVGTVKA
                     VLDGEVQDIHPHDESIQRGVMLEIDVTDWGASETVLFAGSKPLWFL"
     gene            complement(1395..2711)
                     /locus_tag="VF516_27235"
     CDS             complement(1395..2711)
                     /locus_tag="VF516_27235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8111466.1"
                     /translation="MTVHVLWVIVHSLAVVVFVNRYIAGIILRLTRGKKWDETRDDYE
                     PTVTAVIPMFNEGSAIKETLQSLLDSGYPASKLQVICVDDCSTDDSYEHARQVARKSG
                     GRLKVLRNRTNLGKRRSIIRATREADSEIIVSVDSDVVVDPDAVHQLVRRFTEDRIAA
                     VGGWVDVRNKQDNWLTRMQVVKYWYSYFFLKNLEWGFRRVMCLSGCLTAYRRCVLIEL
                     EPVLENRSVLGVPIKYGEDRFLTRQIVKAGYLTTMTLDARCRTFVPSTLSAYFSQQLR
                     WRRSNIVDYTGGFSHVWRLNPVLAIHFFSLFALLLVYPIAVVRALAAHKFFPALVLHT
                     EAVVFFGMLYRWRNRKLPRAERVGPLAFIPLSLLMPVTYALLTPLALFTLDTASWETR
                     NHEEAAPSTALPAAEPAREAVTAEIRVGEPAPAVARARPHAQLPAA"
     gene            3156..4064
                     /locus_tag="VF516_27240"
     CDS             3156..4064
                     /locus_tag="VF516_27240"
                     /inference="COORDINATES: protein motif:HMM:NF021554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2279 domain-containing protein"
                     /protein_id="HEX8111467.1"
                     /translation="MVDTPDALHPDALHPDALHPAGDHKVAAALTLGGIYAGFTTWTY
                     FAWYRKHKPLAQFKWGGDGWLGLHTYAGGADKFGHAWATMSLARVGTELLSQWGGYDR
                     VPSTLAATALSELLFLGVEVKDGFFYEFSFSDATGDTLGALAAVAFSLWPRLDELFDY
                     RVGYWPSVEYRRQFDGGNVNIAEDYSGETYLLALHLGAIRALRDQSWGGWTRFVDVAA
                     GFGTRGYKPDPPSRMLPDYEHHQNLYLGVSLNAQGLFDWLLGGRSEPARKITHGLFEM
                     FNAPFTFVPVLEDSRSPTGTVMPGGA"
BASE COUNT          661 a         1415 c         1392 g          638 t
ORIGIN      
        1 cgcgcggggt caggccgaac tggttggcgg ccacgccgat gatcgtgcgc cccttcagcg
       61 ggggctgcgc catgccgccg gtcccgcccg cgaccaccgt cggatcaccg ggccggctga
      121 cgacttcggg ggacttcgga gcgccgccgc cgatggtgcc ggcgacgggc ggaggacctg
      181 gcggtccgcc tcccgcgcga gtcttgtcct gatcactcat aaacgatgtt tgcctccgca
      241 gaagctgcgc acacctgcca cccttacaac gacatgttgt cgagacccca gacaggccca
      301 tagtacatga gggacccatc ggatcggcga gcgacgcgcg tccaagacga caattcctgc
      361 gcaagaccca ccgtgcgcct ggccgccgcg ccgcgcgcac ccggcggcga ctgcccataa
      421 cccagcgcaa gctatcgaga tcggcggcgc ggacgcggcg ggcgcccgca gcgaattgcg
      481 ccatggccac gccttccggc gggcgatgat ttctgcatgt gaaggcgtgt aatgcgccgc
      541 cggccgagct gggtcgggct agccggctgc ccctgctaca ggaaccacag cggcttggac
      601 ccggcgaaca gcacggtctc gctcgcgccc cagtcggtga cgtcgatctc cagcatcacg
      661 ccgcgctgga tgctctcgtc gtgcgggtgg atgtcctgga cctcgccatc gagcacggcc
      721 ttcacggtgc cgaccttgtg gcaccacacc aggccccaga tgcagccgta cagcggcgtg
      781 ccgagcttga tgttgcgcag gttctggttg gcgacgaacg ccagcgtgac cttgttctcg
      841 atcgccttga ggtacggcga ctgggcgagc cggttgacca ccgcctcctg gtccttgatc
      901 agccgcctct gctgctcgat ctgcaggccg agcggcgcgc gctcgccgac cttgttgtcc
      961 ttgtcgagcc gcgccttctc gacctcgcgg cggaccagcc actgctcggc ggtcctgggc
     1021 gcgtcggccg cggcgccgat ctcggcgagg aaccgctcgt cgtcggcgat cgcgcgctcg
     1081 tgggcggcga ccgactcctc gagcgaggcg agctcgccgg acagccggct cgcccgggtc
     1141 ttggcgtcgg cgagcaggtt cgacgcctcg acgaccttct ggtggttggg cgacagcacg
     1201 accgggcgga cccaggtgtg gtcgaagaag tagaagatgt tcacgaccaa aaacccgacc
     1261 agcccgacga ggatcgcggt cagcgcgacc aggcccgccg ccttgtacac ccggacgatc
     1321 gacggctgca gcgcgatcca gaaccggggg cgactcgagc gagattgcat ttccatggtc
     1381 cttaccacac cgggtcaggc ggcaggcagc tgggcgtgcg gtcgggcacg cgccacggca
     1441 ggcgcgggtt ctccgacccg gatctcggcg gtgaccgcct cgcgggccgg ctcggccgcc
     1501 gggagggcgg tcgacggcgc ggcctcctcg tggttgcggg tctcccagct cgcggtgtcg
     1561 agcgtgaaca gcgcgagcgg ggtgagcagc gcgtaggtca ccggcatcag cagcgacagc
     1621 gggatgaacg cgagcggacc gacccgctcg gcgcgcggca gcttgcggtt gcgccagcgg
     1681 tacagcatgc cgaagaacac cacggcctcg gtgtgcagca cgagcgccgg gaagaacttg
     1741 tgggccgcca gcgcgcgcac caccgcgatc gggtagacca gcagcagcgc gaacagcgag
     1801 aagaagtgga tcgcgagcac cgggttgagc cgccacacgt ggctgaaccc gccggtgtag
     1861 tcgacgatgt tcgaccgccg ccagcgcagc tgctgcgaga agtaggccga cagcgtcgac
     1921 ggcacgaacg tgcggcagcg cgcgtcgagc gtcatcgtcg tgagatagcc ggccttcacg
     1981 atctggcggg tcaggaagcg gtcctccccg tacttgatcg gcacgccgag caccgagcgg
     2041 ttctcgagca ccggctcgag ctcgatcagc acgcaccggc ggtacgcggt caggcagccc
     2101 gacaggcaca tcacgcggcg gaacccccac tcgaggttct tgaggaagaa gtacgagtac
     2161 cagtacttca cgacctgcat ccgggtcagc cagttgtcct gcttgttgcg gacgtcgacc
     2221 cagccgccga ccgcggcgat ccgatcctcg gtgaaccggc gtaccagctg gtgcacggca
     2281 tccggatcga ccacgacgtc cgagtcgacc gagacgatga tctcgctgtc ggcctcgcgg
     2341 gtggcgcgga tgatcgaccg gcgcttgccg aggttggttc ggttccgaag gaccttcagc
     2401 cggccgcccg acttccgggc gacctggcgc gcgtgctcgt agctgtcgtc cgtggagcag
     2461 tcgtcgacgc agatcacctg gagcttgctg gccgggtagc ccgagtcgag caggctctgc
     2521 agggtctcct tgatcgccga cccttcgttg aacatcggga tcacggccgt gacggtcggc
     2581 tcgtagtcgt cccgcgtctc atcccacttc ttacctcggg ttagccgcag gatgatgccg
     2641 gcgatgtagc ggttcacgaa gaccaccacg gccagtgagt ggacgatcac ccagagaacg
     2701 tggaccgtca tcctggcacc ccacgtgcga ggcccacgcc aagatacgcc tgacagtcga
     2761 gtcgaggttt cacggaaggc tatcgatgcg gttaagagac ccggggcacc gcgggacacg
     2821 gtcaaaatta agcgcggtgg cccaaggcct agtaatcgac gcaaactatt atgcttcggg
     2881 gcctaactca agttggttga cgcacgttgc cacctggacc gtgaccaaaa ataccccccc
     2941 tcaggatatt gaggacctac accttgtctt cacccggccc ctatgacatg aggcgacccg
     3001 tgcgtgggct cgcgaccgct gcgttgctga tcgcgtggtt gcaccggccc gtggccgcac
     3061 agccgcccac tcccgatccg cactatgatc cggctgccgc acccacgccc tggctggacc
     3121 aggggacctc gccgatcacg agcagcaccg atcccatggt cgacacacca gacgcgctgc
     3181 acccggacgc gctgcacccg gacgcgctgc accccgcggg cgatcacaag gtcgccgcgg
     3241 cgctgacgct cggcggcatc tatgccggct tcaccacgtg gacctatttc gcgtggtacc
     3301 gcaagcacaa gccgctggcc cagttcaagt gggggggcga tggctggctc ggactgcaca
     3361 cctacgccgg cggcgccgac aagttcggcc atgcgtgggc gacgatgagc ctggctcgcg
     3421 tcggcaccga gctgctcagc cagtggggtg gctacgaccg ggtgccgtcg acgctcgcgg
     3481 cgaccgcgct cagcgagcta ctgttcctcg gcgtcgaggt caaggacggc ttcttttacg
     3541 agttctcgtt cagcgacgcc accggcgaca cgctgggcgc gctggcggcg gtcgcgttct
     3601 cgctgtggcc gcggctcgac gagctgttcg actaccgggt cgggtactgg ccgagcgtcg
     3661 agtaccgccg gcagttcgac ggcggcaacg tcaacatcgc cgaggactac tcgggcgaga
     3721 cctacctgct cgcgctccac ctcggcgcga tccgcgcgct gcgcgaccag tcctggggcg
     3781 gctggacccg gttcgtggac gtcgcggcgg ggttcgggac gcgcggctac aagcccgatc
     3841 cgccgtcgcg catgctgccc gactacgagc accaccagaa cctgtacctc ggcgtgtcgc
     3901 tcaacgcgca gggcctgttc gactggctgc tgggcggccg ctccgagccg gcccgcaaga
     3961 tcacccacgg gctgttcgag atgttcaacg cgccgttcac gttcgtgccg gtgctcgagg
     4021 actcgcgttc gccgaccggc acggtgatgc cgggcggcgc ctgagcgccg atgactcgcg
     4081 cgacgcgacg cgcggggggg tgaatc
//
LOCUS       DASVOR010001435         1652 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_570521,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001435 DASVOR010000000
VERSION     DASVOR010001435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1652
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570521"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..690)
                     /locus_tag="VF516_27245"
     CDS             complement(49..690)
                     /locus_tag="VF516_27245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEX8111468.1"
                     /translation="MADPIRRLIQELARLPGIGEKTATRLAFHLIRSSHQQIRDLSQA
                     LLDATEKIRLCSVCMNMTEVDPCAMCTDPRRDGDTICVVATPSDLIAIDRGGHFRGRY
                     HVLHGLLSPLEGIGPDDIRLAELVRRLATSASGPGSSGPGSGPGNDGGVREVIVATSP
                     SVDGEATAMFIARTLKPLGVQVSRIATGLPVGGELEYSDQATIARALAGRATM"
     gene            complement(697..1032)
                     /locus_tag="VF516_27250"
     CDS             complement(697..1032)
                     /locus_tag="VF516_27250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEX8111469.1"
                     /translation="MSNGKMPDLNALMKQAQKLQGDVQRAQEELAKMTCDGTAGGGLV
                     TATVNGQFEVVRVKIDKSVVDPNDVGMLEDLVTAAVNAAATKIRETTKEKMAQVMGPM
                     AGLPGMPGF"
     gene            complement(1025..>1652)
                     /locus_tag="VF516_27255"
     CDS             complement(1025..>1652)
                     /locus_tag="VF516_27255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111470.1"
                     /translation="AAPPAAIPTEASAASAISGPVSMQPSGPVPRPDTVASGSGPHDA
                     ELGEATRPPSINAEPAARSIAIPASPADALRAWNEVLSELEALRKFSLLGPFQHARVL
                     SWTADLLELGFPVDVHSMGEMARDHADDLRAIVRGLGPAQKNVRVAVRLLDAGESSTA
                     GVRSVLETTQERTSAERSKREAEAREHPITKHVLRTFGAQIKEIKTDV"
BASE COUNT          232 a          598 c          564 g          258 t
ORIGIN      
        1 ttcgaccggc aagccgcccc acgcatgctg caccgcgcgc cggtgcccct acatggtcgc
       61 ccgacctgcc agcgcgcgcg cgatcgttgc ctgatcggag tactcgagct ccccgccgac
      121 gggcagcccg gtcgcgatcc gcgatacctg caccccgagc ggcttgagcg tccgggcgat
      181 gaacatcgcc gtcgcctcgc cgtcgaccga agggctcgtc gcgacgatga cctcgcgaac
      241 gcccccgtcg ttcccggggc cggaaccagg gcccgaagaa ccagggcccg aagcagatgt
      301 ggcgagtcgc cgcaccagtt cggccagccg gatgtcatcc ggaccgatgc cctccagcgg
      361 cgataacagg ccgtgcagga cgtggtagcg gccacggaaa tgtccaccgc ggtcgatcgc
      421 gatcaggtcc gaaggggtgg cgaccacaca gatcgtatcg ccatcgcgcc gcggatccgt
      481 gcacatggcg cacggatcga cctcggtcat gttcatgcag accgagcaca ggcggatctt
      541 ctcggtggcg tcgagcagcg cctgggagag atcgcggatc tgctggtggc tggaccggat
      601 caggtgaaac gccaggcgtg tcgcggtctt ctcgccgatg cccggcaggc gggcgagctc
      661 ctggatcagc ctccggatcg gatcggccat cgacgctcag aagcccggca tgccgggcag
      721 cccggccatc ggccccatga cctgcgccat cttctccttg gtcgtctcgc ggatcttggt
      781 cgccgccgcg ttgaccgccg cggtgaccag gtcctcgagc atgccgacgt cgttcggatc
      841 gacgaccgac ttgtcgatct tcacgcggac gacctcgaac tgaccgttga ccgtcgcggt
      901 caccagcccg ccgccggcgg tcccgtcgca ggtcatcttc gccagctcct cctgggcgcg
      961 ctgcacgtcg ccctggagct tctgggcctg cttcatcaga gcgttgagat cgggcatctt
     1021 tccgttagac atcggtcttg atctccttga tctgcgcacc aaacgtccgg aggacgtgct
     1081 tggtgatcgg atgctcgcgg gcctcggcct cgcgcttgct ccgctccgcc gaggtccgct
     1141 cctgggtggt ctccagcacc gagcgcacgc ccgcggtgct cgactcgccg gcatcgagca
     1201 ggcggaccgc gacccggacg ttcttctgcg ccggccccag gccgcggacg atcgcgcgga
     1261 gatcgtcggc gtggtcgcgg gccatctcgc ccatgctgtg cacgtcgacc gggaagccga
     1321 gctcgagcag gtccgccgtc cagctcagca cgcgcgcgtg ctggaacggc ccgagcagcg
     1381 agaacttgcg cagcgcctcc agctcggaga gcacctcgtt ccacgcccgg agcgcgtccg
     1441 ccgggctggc cgggatcgcg atgctgcgcg ccgccggctc ggcgttgatc gacggtgggc
     1501 gcgtcgcctc gcccaactcg gcatcgtgcg ggccggagcc cgaggcgacc gtgtcgggcc
     1561 tgggcaccgg tcccgacggc tgcatcgaca ccgggcccga gatcgcgctg gccgcggagg
     1621 cctcggtggg gatggccgct ggcggcgcgg cg
//
LOCUS       DASVOR010001436         1500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_276879,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001436 DASVOR010000000
VERSION     DASVOR010001436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276879"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..>1500)
                     /locus_tag="VF516_27260"
     CDS             complement(33..>1500)
                     /locus_tag="VF516_27260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111471.1"
                     /translation="TGSLGPAPAIQPPVTARDADDRVRGRVEKRASRPASPPAAGAVL
                     DARVAAGDQKMKQITQSVVHSDKTIVIEGITNRGDTEGNRRANDRANIVKNQLIDQGV
                     APARIKIVTKVDPNQPERVRLLAQAPRAGEPRSAGGKALDVDAQPVGESHFENKHPMT
                     VDRGSSAMVSMVRDVTQGEVVYLYDAESERGNARFAFRAVRFKNPTDSTLETGPVTVY
                     GQERFIGEGLTEPIPPHASAVVPFALDRQIVIERTDGQDDKIAKLISLQRGILTAEVQ
                     HVRRRRLAITNRLGQVARVYVRHSVTKGWKLLESPPAFERIGDAHLFEVQIPPGGTQQ
                     VEIAEATPIQSTLDLSADVTLEMMKVWVEAPEVTRELKDQLKKLLAIHRTLVDLAQEQ
                     DSMRRRLADYRERMDELHGQIVTLQAVKTGGELMGHLKTKMKEISDRVQRTTIDIVDQ
                     EEKIMLARVQFQDALAELALPDAMQTKPAAKPMAMTKS"
BASE COUNT          176 a          509 c          544 g          271 t
ORIGIN      
        1 ggcgctgtga tcgaagtcgg ccgcgctggc agtcagctct tggtcatcgc catgggcttc
       61 gcggcgggct tggtctgcat cgcgtcgggc agcgcgagct cggcgagcgc gtcctggaac
      121 tggacgcggg cgagcatgat cttctcctcc tggtcgacga tgtcgatcgt ggtccgctgc
      181 acgcggtccg agatctcctt catcttggtc ttgaggtgcc ccatcagctc gccgccggtc
      241 ttgaccgcct ggagcgtgac gatctggccg tgcagctcgt ccatccgctc gcggtagtcg
      301 gcgagccggc gccgcatgct gtcctgctcc tgggcgaggt cgaccagcgt cctgtggatc
      361 gcgagcagct tcttgagctg gtccttgagc tcgcgcgtga cctcgggcgc ctcgacccag
      421 accttcatca tctcgagggt gacgtcggcc gacaggtcga gcgtgctctg gatcggcgtg
      481 gcctcggcga tctccacctg ctgggtcccg ccgggtggga tctggacctc gaacaggtgg
      541 gcatcgccga tccgctcgaa cgccggcggc gactcgagca gcttccagcc cttggtcacg
      601 gagtggcgga cgtacaccct ggcgacctgg cccagccggt tggtgatcgc gagccggcgg
      661 cggcggacgt gctggacctc ggcggtgagg atgccgcgct ggagcgagat cagcttcgcg
      721 atcttgtcgt cctgcccgtc ggtgcgctcg atgacgatct ggcgatcgag cgcgaacggc
      781 acgaccgccg aggcgtgggg tgggatcggc tcggtcaggc cctcgccgat gaaccgctcc
      841 tggccataca cggtgaccgg gccggtctcc agcgtcgagt cggtcgggtt cttgaagcgc
      901 accgcgcgga acgcgaaccg cgcgttgccg cgctcggact cggcgtcgta gaggtagacg
      961 acctcgccct gggtgacgtc gcgcaccatc gagaccatcg ccgacgagcc acggtcgacc
     1021 gtcatcgggt gcttgttctc gaagtggctc tcgccgaccg gctgggcgtc gacgtcgagc
     1081 gccttgccgc ccgcggaccg cggctcgccg gcgcgcggcg cctgggcgag cagccgcacc
     1141 cgctcgggct ggttgggatc gaccttggtg acgatcttga tccgggccgg cgccacgccc
     1201 tggtcgatca gctggttctt gacgatgttg gcgcggtcgt tggcgcgccg gttgccctcg
     1261 gtgtcgccgc ggttggtgat gccctcgatc acgatcgtct tgtccgagtg cacgaccgac
     1321 tgcgtgatct gcttcatctt ctgatcgccg gcggcgaccc gggcgtcgag cacggccccc
     1381 gccgcgggtg gggaagccgg cctggacgcc cgcttctcca cccggccgcg cacgcggtcg
     1441 tcggcgtcgc gcgcggtcac cggcggctgg atcgcggggg ccgggcccag cgagccggtg
//
LOCUS       DASVOR010001437         2092 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_160633,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001437 DASVOR010000000
VERSION     DASVOR010001437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160633"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VF516_27265"
     CDS             <1..716
                     /locus_tag="VF516_27265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111472.1"
                     /translation="GHNAYFLGASLPMTSKHAAVQDTCVGCHMTLQPKGYLSHGAPAP
                     SGHLFRIEDADQQALCAKCHGSAVDGAGIQGQVEAQLVAVAAKMGNAVKAKVNGFSGG
                     LVRVRAWDPATDLYSSTSASNLVLDVVANPVTAVDVEEIHGQIGFLLHFATPITVPFV
                     NAAGNPVPSKALTSFGVQMGAIKDNAATPAALYLLSGNLVRAGWNYFLVEGDQSKGLH
                     NPSFVTAVLNATLAKDLSN"
     gene            811..1842
                     /locus_tag="VF516_27270"
     CDS             811..1842
                     /locus_tag="VF516_27270"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8111473.1"
                     /translation="MRERGLEYKVGLLILTSTAILLAFIFVLGNYSLSSGFTIHVDYD
                     YVGSLQAGAPVKLSGIKVGKVKAVELYGGRQDPAIGKRVQVRVTAWVEDRAADAIRGD
                     AEFFINTAGVLGEQYLEIVPGKDWTHPPIQPGTIVHDPHFVHDPPRTDLVVARLYEVL
                     DGVSGVLRDDRDEIKHLLRNGASAVAEVNQLLVDNRARIGELIGDTTALARQATATLD
                     KVNAGLGDGKPVASLLASADGALVAARGSLQTLTPPAASFLTDATRVTGLITDQRLGS
                     VDRALDGLAGAAGGANTLLGTANAIASDLRAGKGTVGNLLSRDELYTDLRELIRDLKR
                     NPWKVFWKE"
     gene            1845..>2092
                     /locus_tag="VF516_27275"
     CDS             1845..>2092
                     /locus_tag="VF516_27275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111474.1"
                     /translation="MLLAQDPRLARRVGALTLLAMAAVSAGLVFLLDRGGLGPTIRFR
                     VMLRSSGGLHDRAPLIVAGRPVGRIAAITPMPHGAPGPL"
BASE COUNT          312 a          776 c          706 g          298 t
ORIGIN      
        1 ccggccacaa cgcgtacttc ctcggcgcca gcctgccgat gacttcgaag cacgccgcgg
       61 tccaggacac ctgcgtcggc tgccacatga cgctgcagcc caagggctac ctgtcgcacg
      121 gcgcgccggc cccgagcggc cacctgttcc gcatcgagga cgccgaccag caggcgctgt
      181 gcgcgaagtg ccatggcagc gcggtcgacg gcgccgggat ccagggccag gtcgaggccc
      241 agctcgtcgc ggtcgcggcg aagatgggca acgcggtcaa ggccaaggtc aacggcttct
      301 cgggcggcct ggtccgggtc cgggcctggg atcccgcgac cgacctgtac tccagcacgt
      361 cggcctccaa cctggtgctc gacgtcgtcg ccaacccggt gaccgcggtc gacgtcgagg
      421 agatccacgg ccagatcggc ttcctcctgc acttcgcgac cccgatcacg gtgccgttcg
      481 tcaacgcggc cgggaacccg gtgccgagca aggcgctgac cagcttcggc gtgcagatgg
      541 gcgcgatcaa ggacaacgcg gccaccccgg cggcgctgta cctgctgtcc ggcaacctgg
      601 tccgcgccgg ctggaactac ttcctggtcg agggcgatca gagcaagggc ctgcacaacc
      661 cgagcttcgt caccgccgtg ctgaacgcca cgctggccaa ggacctgtcg aactagtgct
      721 gcaacgctgc tagccacgca cgtggactgc gatggcaccg aggcctccgc atgctgcggg
      781 ggccccggca tttttcggct acgctttgtc atgcgcgaac gtggcctcga gtacaaggtc
      841 gggctcctga tcctgacgtc gacggcgatc ctgctcgcct tcatcttcgt cctgggcaac
      901 tactcgctga gctcggggtt cacgatccac gtcgactacg actacgtcgg cagcctccag
      961 gccggcgcgc cggtcaagct gtcggggatc aaggtcggca aggtcaaggc ggtcgagctc
     1021 tacggcggcc ggcaggatcc ggcaatcggc aagcgagtcc aggtccgggt caccgcgtgg
     1081 gtcgaggacc gcgcggccga cgcgatccgc ggcgacgccg agttcttcat caacaccgcc
     1141 ggcgtgctcg gcgagcagta cctcgagatc gtgcccggca aggactggac tcacccgccg
     1201 atccagcccg gcacgatcgt gcacgacccg cacttcgtgc acgacccgcc gcgcaccgac
     1261 ctggtggtcg cgcggctcta cgaggtgctc gacggcgtgt ccggcgtgct gcgcgacgac
     1321 cgcgacgaga tcaagcacct gctccgcaac ggcgcgagcg cggtcgccga ggtcaaccag
     1381 ctcctggtcg acaaccgcgc ccggatcggc gagctgatcg gcgacaccac cgcgctcgcc
     1441 cggcaggcga cggccacgct cgacaaggtc aacgccggcc tgggggacgg caagccggtc
     1501 gccagcctgc tcgcgagcgc cgatggcgcg ctggtcgccg cgcgcggctc gctccagacc
     1561 ctgaccccac cggccgcgag cttcttgacc gacgcgaccc gggtgactgg cctcatcacc
     1621 gatcagcggc tcggcagcgt cgaccgcgcg ctcgacggcc tggcgggcgc cgccggcggg
     1681 gccaacacgc tgctcggcac cgccaacgcg atcgcctccg acctgcgcgc cggcaagggc
     1741 acggtcggca acctgctgtc gcgcgacgag ctgtacaccg atctgcgcga gctgatccgc
     1801 gatctcaagc gcaatccctg gaaggtgttc tggaaggaat agcgttgctg cttgcccagg
     1861 atccccggct cgcccgccgg gtcggcgcgc tcaccctctt ggccatggcc gcggtgagcg
     1921 ccggcctggt gttcctgctc gaccgcggcg ggctcggccc gacgatccgg ttccgcgtga
     1981 tgctccggtc cagcggtggc ctgcacgacc gcgcgcccct gatcgtcgcc ggtcggccgg
     2041 tcggccggat cgcggcgatc acgccgatgc cgcacggcgc gcccggcccg ct
//
LOCUS       DASVOR010001438         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_482493,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001438 DASVOR010000000
VERSION     DASVOR010001438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482493"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..672)
                     /locus_tag="VF516_27280"
     CDS             complement(<1..672)
                     /locus_tag="VF516_27280"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8111475.1"
                     /translation="MPASPRIVIDDRFRRLTLPTAGLDLLWTGARWAEGPAYFAAGRY
                     LVWSDIPNDRMMRWDETDGSVSVFRSPCHHTNGHTVDREGRLVSCEHQARCVSRTEHD
                     GTRTVLVDRIAGRRLNSPNDVVVKSDGTIWFSDPTYGIDTDYEGDAAPSEIGASHVYC
                     CDPATGACAAVTTDFVQPNGLAFSPDERQLYIADTGVSHVPGGPAHIRQFAVASDGRT
                     LSGGGV"
     gene            complement(672..>1581)
                     /locus_tag="VF516_27285"
     CDS             complement(672..>1581)
                     /locus_tag="VF516_27285"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8111476.1"
                     /translation="IGCNAVLLAAGALYDANFLSPGYLLQQLQVASFLGVIATGVLLV
                     VLLGHIDLSIPWVVTLGGMMSTATAGHDGLVGSLAIPFAVACGVGIGIVNGIGVAYLR
                     VPSMIFTLATNTIAQGLMVVHTSGFAPQDHATPAMHFLAVERSILGIPNAVWVWAAIG
                     VATVAMLRRTGFGRRIYAIGNGERAVYLSRGHVGRVVVACFAISGACSAFAGVLLAGY
                     STKAYQAMGDPYLLPAIAAVVLGGARILGGRGSYLGTVAGVVLVTLLQSILSIVQMPE
                     AGRQVIYGTVIIIMLLAYGRGPKGVT"
BASE COUNT          257 a          535 c          593 g          196 t
ORIGIN      
        1 caccccgccg cccgacagcg tccggccgtc ggacgccacc gcgaactggc ggatgtgggc
       61 cgggccgccg ggcacgtggc tcacgccggt gtcggcgatg tagagctggc gctcgtcggg
      121 cgagaacgcc aggccgttgg gctggacgaa gtcggtggtg accgccgcgc acgcgccggt
      181 ggcgggatcg cagcagtaga cgtgggaggc gccgatctcc gacggcgcgg catcgccctc
      241 gtagtcggtg tcgatgccgt aggtcggatc ggagaaccag atcgtgccgt cggacttgac
      301 gacgacgtcg ttgggcgagt tgagccgccg gccggcgatc cggtcgacca gcacggtgcg
      361 ggtgccgtcg tgctcggtcc ggctcacgca gcgggcctgg tgctcgcacg acaccaggcg
      421 gccctcgcgg tcgacggtgt ggccgttggt gtggtggcac ggcgagcgga acaccgacac
      481 cgagccgtcg gtctcgtccc accgcatcat gcggtcgttc gggatgtcgg accacacgag
      541 gtagcggccg gccgcgaagt acgccggacc ctcggcccac cgcgcgccgg tccacagcag
      601 gtcgagcccg gcggtgggca aggtgagacg gcggaaccga tcgtcgatca cgatgcgggg
      661 cgaggcgggc atcaggtcac tcccttcggg ccgcggccgt acgcgagtag catgatgatg
      721 atgaccgtgc catagatcac ctggcgcccg gcctcgggca tctggacgat cgacaggatc
      781 gactggagca gcgtgaccag gacgactccc gcgacggtgc ccaggtagct gccgcggccg
      841 ccgaggatgc gggcgccgcc gagcacgacc gccgcgatcg ccggcaacag gtacggatcg
      901 cccatcgcct ggtaggcctt ggtcgagtag ccggcgagca gcacgccggc gaacgccgag
      961 caggcgcccg agatcgcgaa gcacgcgacc acgacccggc cgacgtggcc gcgcgacagg
     1021 tacacggcgc gctcgccgtt gccgatggcg tagatccgcc ggccgaaccc ggtgcggcgc
     1081 agcatcgcca cggtcgcgac gccgatcgcc gcccataccc ataccgcatt gggaatgccg
     1141 aggatcgagc gctcgaccgc gaggaagtgc atcgccggcg tcgcgtggtc ctgcggggcg
     1201 aacccgctgg tgtggaccac catcaggccc tgcgcgatcg tgttggtggc gagcgtgaag
     1261 atcatcgacg ggacgcgcag gtaggcgacg ccgatgccgt tgacgatccc gatcccgacc
     1321 ccgcaggcga cggcgaacgg gatcgcgagc gagccgacca ggccgtcgtg gccggcggtg
     1381 gcggtcgaca tcatcccgcc cagggtcacg acccacggga tcgacagatc gatgtggccg
     1441 agcaggacga ccagcagcac gccggtggcg atcacgccga ggaacgacgc gacctggagc
     1501 tgctggagca ggtagcccgg cgacaggaag ttcgcgtcgt acagcgcgcc cgcggcgagc
     1561 agcacggcgt tgcagccgat c
//
LOCUS       DASVOR010001439         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_219394,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001439 DASVOR010000000
VERSION     DASVOR010001439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219394"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..310)
                     /locus_tag="VF516_27290"
     CDS             complement(<1..310)
                     /locus_tag="VF516_27290"
                     /inference="COORDINATES: protein motif:HMM:NF015862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HEX8111477.1"
                     /translation="MPNPYGQISLEVARKVADAAIAEGKKSGWNIAAAVVDPAGELVF
                     FERMDNTQSASIVIAQEKARTAAKFKRPSKALEDALAGGRQAILALPGATPVEGGIPL
                     V"
     gene            complement(461..1429)
                     /locus_tag="VF516_27295"
     CDS             complement(461..1429)
                     /locus_tag="VF516_27295"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEX8111478.1"
                     /translation="MTTPALEPPTLDDIRAAHARVGREVVRTPLVRLGVDGPAEIYLK
                     LENLQPIGSFKLRGAANAMAQARDAEIAHGVFTASAGNMAQGVAWCARARGVPCGVVV
                     PDTAPEAKLSAITRLGAEIHKVPFADWWNVLVTRQHPRFAGRLFIHPVSDRAVMAGNG
                     TIGVEIIEALPAPDAVIIPYGGGGLTTGIASALRALSPATRIYTAEPETGAPMAASWA
                     AGELREAAYTPSFVDGCGSRSLLAEIWPIVRRLVDGAFAVPLAEIAAAIRILVERNRV
                     VAEGAGATPVAAALAGRAGTGKVVCVVSGGNLDSAKLARIVQGELP"
BASE COUNT          218 a          583 c          534 g          217 t
ORIGIN      
        1 tgaccagcgg gatgccgccc tcgaccggcg tggcgcccgg cagcgcgagg atcgcctggc
       61 gaccgccggc cagcgcgtcc tccagcgcct tgctcggccg cttgaacttg gcagcggtgc
      121 gcgccttctc ctgcgcgatc acgatgctgg ccgactgggt gttgtccatg cgctcgaaga
      181 acaccagctc gccggcggga tcgaccaccg ccgcggcgat gttccagccg ctcttcttgc
      241 cctcggcgat cgcggcgtcg gcgaccttgc gcgcgacctc caggctgatc tgcccgtagg
      301 gattgggcat ctgggcgtgc gcactgcgcg acgcaaggct cagaaccacg actgcaaata
      361 cgattgtcac gcgcagagat gtcttcatcg cttgccgcct ccggtagatt gggcggcgcg
      421 cagtatagtc aattgcgctc gcgcgtacag cgatgccacg tcacggcaac tcgccctgaa
      481 cgatccgggc gagcttggcc gagtccaggt tcccccccga taccacgcac acgaccttgc
      541 cggtgcccgc ccggccggcc agcgcggcgg cgaccggcgt cgcaccggcg ccctcggcga
      601 cgacccggtt gcgctcgacc aggatccgga tcgcggccgc gatctcggcg agcggcaccg
      661 cgaacgcacc atcgaccagc cggcgcacga tcggccagat ctcggcgagc aggctgcggc
      721 tgccgcagcc gtcgacgaag ctcggcgtgt acgcggcctc gcgcagctcg ccggcggccc
      781 acgacgccgc catcggcgcg ccggtctcgg gctcggcggt gtagatccgg gtcgccggag
      841 acagcgcgcg gagcgccgac gcgatgccgg tggtgagccc gccgccgccg tacgggatga
      901 tcaccgcgtc gggcgccggc agcgcctcga tgatctcgac gccgatcgtg ccgttgccgg
      961 ccatcaccgc gcggtcggag accgggtgga tgaacagccg ccccgcgaac cgcggatgct
     1021 gccgggtcac cagcacgttc caccagtccg cgaacggcac cttgtggatc tcggcgccga
     1081 gccgcgtgat cgccgacagc ttggcctcgg gcgcggtgtc ggggaccacg acgccgcacg
     1141 gcacgccgcg ggcccgcgcg caccacgcca cgccctgggc catgttgccc gcgctcgcgg
     1201 tgaacacccc gtgcgcgatc tcggcgtcgc gggcctgcgc catcgcgttg gccgcgccac
     1261 gcagcttgaa cgagccgatc ggctggaggt tctccagctt gaggtagatc tcggccgggc
     1321 cgtcgacccc gagccggacc agcggcgtgc gcacgacctc gcggccgacc cgtgcgtgcg
     1381 cggcgcggat gtcatcgagt gtcggtggct ccagcgcggg agtcgtcatg gccgtactac
     1441 acccgatccg ggcccgccgc gcagccgcgg agcgcgacaa tcggcgttgc aatcatcaac
     1501 gcaacgcgcg ccgcgatcgc gatgcgttgc catcatcaac gcgactcgct cc
//
LOCUS       DASVOR010001440         6210 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_452951,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001440 DASVOR010000000
VERSION     DASVOR010001440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6210
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452951"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="VF516_27300"
     CDS             complement(<1..635)
                     /locus_tag="VF516_27300"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8111479.1"
                     /translation="MSKPAWLVILAACGGAHGEPASAPAADRHAMQHHFESAEVCARS
                     FDDPARDRWQQPERVLEALELGPGMVIADIGAGTGYFSVRLARAVPDGQVIATDIEPD
                     MIRYLTERAAREQLPNLRAVRTPPADPQLAAGTVDRILVVDVWHHIDDRAGYARKLAQ
                     ALRPGGKLAIVDFTREATHGPPPEMRLTPEQVIGELRQAGLAAALSPTVLP"
     gene            complement(706..1074)
                     /locus_tag="VF516_27305"
     CDS             complement(706..1074)
                     /locus_tag="VF516_27305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111480.1"
                     /translation="MFTRRELLTRGTTLLLLIPVVGCSSSSDQTGGGSCAGIQSTSSI
                     DAAHTHTVCVLTSDLTSPPAAGVTYTTSNVGDHTHKVMLTAADLTTINGGQTVTVTST
                     TDPDPIDNVTHSHIFMIKKM"
     gene            complement(1179..2540)
                     /locus_tag="VF516_27310"
     CDS             complement(1179..2540)
                     /locus_tag="VF516_27310"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8111481.1"
                     /translation="MNHRGLVSLLVIAACGGAPPHDGAGAPAAGGGRVVVVSIDGLMP
                     DAYLAPDAHGLAIPTLRSLVARGAAGRVHGVMPTVTYPSHTTLVTGVPPRIHGIVSNK
                     PLDALGRNFDGWRWYAEDIRVPTLYQAVEAQHRTAALIVWPVTVGARASVVVPEYWRA
                     GGPDDQKLLRALSTPGLLDEVARAEPALWQALTPPDIEDRAQIAIARHVLATRRPDLM
                     LVHMFELDDAQHDHGPWSAEARATMEAIDRELGALLGDLERSPDWKRTTLVVVSDHGF
                     APIDHEIRLGVLFARHGLVRSDRDGAVTEAGVGVIVGGGSALFYVLDPKRREAIDAAL
                     GELGDRVARRIGHDALVARGGEPAAAFALVAAPGHGFSDKRTGDLIAALSPHGTHGWP
                     PDDPAMAASLIAFGPGVPHVALGTVEMIDIAPTIARWLGVPLASATGAPIARLIATSA
                     GAR"
     gene            complement(2655..3443)
                     /locus_tag="VF516_27315"
     CDS             complement(2655..3443)
                     /locus_tag="VF516_27315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111482.1"
                     /translation="MWQRLTSVHCKESYLQDLFDHWGQATCLRVGVPPWLRGFSGSNA
                     RRPRSAFGYQFTTDAEYEIGSRRYVIELKHAVKYEPLALAEVLHHAEWIRRYERDHTE
                     VVPVIVTQFSCWLRLAIAALGRRMDGLGALRYYEVDVLGQDDPAVIWFDEPLAPWVPA
                     ERPPFLLEHPETARLHWHHVATTRTWFGLDEIVEERPLVLEVPYVTVSEIDHGGFVLW
                     EGTAAPNGTPRSTHLRSRYYAWCPGGNAEARDGVRPSWGAGLAD"
     gene            4318..5061
                     /locus_tag="VF516_27320"
     CDS             4318..5061
                     /locus_tag="VF516_27320"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8111483.1"
                     /translation="MRSSRPSRKPKRSAQQELFRRGGKRRGAGRKPRGTRAGERHEAR
                     PEFKPYHPLHVVMRVAPEVGSLRRRTMYKALREATITAALRERFRIVHISLQRTHVHL
                     LVEADHKTALARGMQGFTIAAARHINTALGNGARRRRGQVFADRYHVEVITTPTRAHH
                     AISYVLSNWRKHGEDRHGLASTWLVDPFSSGSSFPDWQELQGQPWMWPLRETYDPLIV
                     RRPQSWLLREGWKLGGVPISAHDVPSRPR"
     gene            complement(5391..5816)
                     /locus_tag="VF516_27325"
     CDS             complement(5391..5816)
                     /locus_tag="VF516_27325"
                     /inference="COORDINATES: protein motif:HMM:NF013522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase family protein"
                     /protein_id="HEX8111484.1"
                     /translation="MARTGSSLTPDDKRVLIERVTRAAAEAEGVPVDEMTWVLIHEIA
                     RDGWGERGKPLAAGTSRPIVEIATPVGWTTMARKHEMIQRVATAAAEAAGTDGVHYVI
                     VHEVPDGGWGWEGRQVPRSEYQQFAAPDIASAAPERARS"
     gene            complement(5826..>6210)
                     /locus_tag="VF516_27330"
     CDS             complement(5826..>6210)
                     /locus_tag="VF516_27330"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829720.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8111485.1"
                     /translation="SGIRPRARIRAFASVGSEPVIMLTGPAPAARKALAIAGLTPADI
                     DLWEINEAFAAVVLQTIRALEIDPERVNVNGGAIALGHPLGATGAMLLGTALDELERR
                     DRQLAVIALCIGGGQGIATVIERVR"
BASE COUNT          963 a         2162 c         2161 g          924 t
ORIGIN      
        1 tcgggcagca cggtcggcga cagcgccgcc gcgagcccgg cctggcgcag ctcgccgatc
       61 acctgctcgg gggtgagccg catctccggc ggcggcccgt gcgtggcctc gcgggtgaag
      121 tcgacgatcg cgagcttgcc gccgggccgc agcgcctggg ccagcttgcg cgcgtagccg
      181 gcccggtcgt cgatgtggtg ccagacgtcg accacgagga tgcgatccac cgtgcccgcc
      241 gcgagctgcg gatcggccgg cggcgtccgc accgcgcgca ggttaggcag ctgctcgcgc
      301 gcggcgcgct cggtgaggta gcggatcatg tcgggctcga tgtcggtcgc gatcacctgg
      361 ccgtcgggca ccgcgcgcgc cagccgcacc gagaagtagc cggtccccgc gccgatgtcg
      421 gcgatcacca tccccgggcc gagctcgagc gcttccagca cccgctccgg ctgctgccag
      481 cggtcgcgcg ccggatcgtc gaacgaccgg gcgcagacct cggcgctctc gaagtggtgc
      541 tgcatggcgt gccggtcggc cgccggcgcg ctcgccggtt cgccgtgcgc gccaccgcac
      601 gccgcgagga tcacgagcca ggcgggcttg gacatccgcg gcatcatcgc gccgcaccgg
      661 cagcgacgca agtgagcccg ccggggcggt ccggcgctgg agccatcaca tcttcttgat
      721 catgaagatg tgcgagtgcg tgacgttgtc gatcggatcc ggatcggtcg tcgacgtcac
      781 cgtcaccgtc tggccgccgt tgatcgtcgt caggtcggcg gcggtcagca tgaccttgtg
      841 ggtgtggtca ccgacgttgc tcgtcgtgta cgtcacgccg gcggcgggcg ggctcgtcag
      901 gtcgctggtc agcacgcaca ccgtgtgggt gtgcgccgcg tcgatcgacg atgtcgactg
      961 gatgccggcg cagctcccgc cgcccgtctg atcgctgctc gacgagcaac ccacgaccgg
     1021 gatcaggagc agcagggtgg ttccacgggt cagcagctcg cgacgtgtga acatgagatc
     1081 ctcgacttcc cacaacacga gggccgcgcg cggccccggt cgcaaccgcg gcctgcaaca
     1141 cagcgttcca cgcaggcgct gaacgccggc cgagtgtgct accttgcccc ggcgctcgtc
     1201 gcgatcagcc gcgcgatcgg ggcgccggtg gccgaagcca gcggcacccc cagccagcgc
     1261 gcgatcgtcg gcgcgatgtc gatcatctcg accgtgccga gcgccacgtg cgggaccccg
     1321 gggccgaacg cgatcagcga cgccgccatc gccggatcgt cgggtggcca gccgtgggtc
     1381 ccgtgcggcg agagcgcggc gatcaggtcg ccggtgcgct tgtccgagaa gccgtggccg
     1441 ggggccgcaa ccagcgcgaa cgcggccgcg ggctcgccgc cgcgcgccac cagcgcgtcg
     1501 tgcccgatcc ggcgcgcgac gcggtcgccg agctcgccga gcgcggcgtc gatcgcctcg
     1561 cgccgcttcg gatcgagcac gtagaacagc gcgctgccgc cgcccacgat cacgccgacg
     1621 ccggcctcgg tcaccgcgcc gtcgcgatcg ctgcgcacca ggccgtgccg ggcgaacagc
     1681 acgccgagcc ggatctcatg atcgatcggc gcgaagccgt gatccgagac cacgaccagc
     1741 gtggtccgct tccagtcggg cgagcgctcg aggtcgccga gcagcgcgcc gagctcgcga
     1801 tcgatcgcct ccatcgtcgc ccgcgcctcc gcgctccacg gcccgtggtc gtgctgcgcg
     1861 tcgtcgagct cgaacatgtg caccagcatg aggtcgggcc ggcgcgtcgc cagcacgtgg
     1921 cgcgcgatcg cgatctgggc gcggtcctcg atgtccggcg gcgtcagcgc ctgccacagc
     1981 gccggctcgg cgcgcgcgac ctcgtcgagc agcccgggcg tcgacagcgc gcgcagcagc
     2041 ttctggtcgt ccggcccgcc ggcgcgccag tactccggga ccacgacgct ggcgcgcgcc
     2101 ccgaccgtca ccggccacac gatcagcgcg gccgtgcggt gctgcgcctc gacggcctgg
     2161 tacagcgtcg gcacccggat gtcctcggcg taccaccgcc agccgtcgaa gttgcggccg
     2221 agcgcgtcga gcggcttgtt gctcacgatg ccgtggatcc gcggcggcac cccggtcacc
     2281 agcgtggtgt gcgacgggta cgtcaccgtc ggcatcacgc cgtgcacgcg acccgccgcg
     2341 ccgcgcgcca ccagcgagcg caaggtcggg atcgccaggc cgtgcgcgtc gggcgcgagg
     2401 tacgcatcgg gcatcagccc gtcgatgctg accacgacca cccggccgcc gccggccgcc
     2461 ggcgcgccgg caccgtcatg cggtggagca ccgccgcacg cggcgatcac gagcaagctg
     2521 accagccccc ggtggttcat gccagtgatt ccatcacacc gccgtgacgg ccgggtgaca
     2581 gcgcaggtcc gaacgttgct cccggcgacg aacgcggaag cccggacgat ggtgtgttcg
     2641 acgccttccg gtgctcaatc ggcgaggcct gcgccccacg acggccgcac accatcgcgc
     2701 gcctccgcat tacccccagg gcaccacgcg tagtagcgac tcctcagatg ggtgctgcgg
     2761 ggggtgccgt tcggagcagc tgtgccttcc cacagcacga agccgccatg gtcgatttcg
     2821 ctcaccgtca cgtagggcac ttcaagcacg agcggacgct cttccacgat ctcgtcgagc
     2881 ccgaaccagg tacgcgttgt cgcaacatgg tgccaatgca ggcgcgcagt ctctggatgc
     2941 tcgagcaaga acggcggtcg ctccgcgggt acccacggcg caagcggctc atcgaaccag
     3001 atcaccgcag gatcatcctg gccgagaaca tcaacctcgt aatagcgaag cgcaccgagg
     3061 ccatccatac gacgcccgag cgcagcaatg gcgagtcgca gccagcagct gaactgcgtc
     3121 acgatcacgg gtaccacctc ggtgtgatcg cgctcgtacc ggcggatcca ctccgcgtga
     3181 tggagcactt ccgcgagcgc gagcggctcg tacttgacgg cgtgcttgag ctcgatgacg
     3241 tagcgccgcg acccgatctc gtactccgca tcggtggtga actggtaccc gaacgccgat
     3301 cgcgggcggc gcgcgttcga cccgctgaac ccacgcaacc acggcggcac ccccacgcgc
     3361 aggcacgtcg cctggcccca atgatcgaac aggtcctgta gatagctctc cttacagtgc
     3421 acagaagtca gccgctgcca cacatagcac ctcctcggtc atcgtacgct cacgagcttc
     3481 caacgccaag tcggggcgcc gcaccggtcg ggccaagcgg cgttggccag cgcgacggct
     3541 gcatgacggc tgcggccagg aacttcggga gaccgcgtgg acggccctgt gactggcccg
     3601 tcgaagctga cccggctgac caggacggcc cactcacagt gcatcgccct gcgcggacgc
     3661 tcccttttcc agttttttgg ggggaggatc cattcggatt cccggagaaa ggcacgctcg
     3721 gtgcagtcgg cgttgcatcc aatcactcgg ggctctcgga tagcgagtgc ttcccgctga
     3781 gcacggatct ttccttccct tggttcgtcg gtgctcgaac accagcgggc cgttatggac
     3841 atgtctcgtg tctatggagc gcaagatgtg gtcgagccga ctctcggtca tctcttctcg
     3901 accaaagctc aaatataaat gcaagataag gacctggaat cgagggcaag gactggggca
     3961 acggaaggac gactgtgagc cgcgtctggg tcaaactggc gcccagcgac gtgggtcatc
     4021 aggcggcccc gttctccaca ggccgccacc atgttctgca agatcgcgct cgaggattcg
     4081 tcggatgctg gcacgatcag gcaagacgca gtcaatgtca gatcggccac tcagcgtcgt
     4141 gccggtctgt ccggcaagcg acgcgcgctc gcagtgtctg tcaccatgca atgacaagtg
     4201 tccttgcacg gatgatggga tggccggccc cgtacgctcg gataacgcga gaatatgaat
     4261 gactattagt cctgcccaat cattgacaga ccgcgccttc gtggtcatga tgcgcatatg
     4321 aggtcgtcga ggccgagccg caagccgaag cgaagcgctc agcaggaact gtttagacgc
     4381 ggcggcaagc ggcgcggtgc tgggcggaag cccaggggta cacgcgccgg cgagcggcac
     4441 gaggcgcggc cggagttcaa gccgtatcat ccgctacacg tcgtgatgcg ggttgcaccg
     4501 gaggtgggga gcttgcggcg gcgcacgatg tacaaggcgc tgcgcgaggc cacgatcacg
     4561 gcggcgctgc gcgagcggtt ccggatcgtg cacatcagcc tgcagcggac acacgttcac
     4621 ctgctcgtcg aggccgatca caagacggcg cttgcccgtg ggatgcaggg cttcacgatc
     4681 gcggcagcga ggcacatcaa cacagcgctc ggaaacggcg cacgccggcg ccgcggccag
     4741 gtgttcgccg accggtatca cgtcgaggtg atcacgactc cgacgcgggc gcatcacgcg
     4801 atttcttacg tcctctcgaa ctggagaaag catggtgaag accgccatgg attggcgagc
     4861 acgtggctcg tcgacccgtt ttccagtggg agctcgttcc ccgactggca ggagctgcaa
     4921 ggccagccgt ggatgtggcc cttgcgcgag acctatgatc cgctgatcgt tcgtcggccg
     4981 cagagctggt tgcttcggga gggctggaag ctcggcggag tcccgatcag cgcgcacgat
     5041 gtaccgagca gaccacgttg aaggggctct cgcacgacag ggatgggtgt aaaccgcggt
     5101 cgtgtggggt ccagctgcgc ccgtccgcag taccccttca gggcgagcag accaaggaca
     5161 gcctaccttg tggtaacgta cctggacggg accacgctct gcgtcgcgga tccgagggga
     5221 cagcacgaga tccgcttcat gggcgatcgc aaccgctcgg cgccgatcag cctggagcct
     5281 ggcgacaggg gtcagccacc cggtgactgc cgccgagtgg ggcgcgcatg cggtccatgg
     5341 cggcgagccg cgggtcctgg cccacctagg gcgtctgccg ccctgccgtc tcaggagcgt
     5401 gcgcgttccg gcgctgcaga cgcgatgtcc ggtgcagcga actgctggta ctcggatcgc
     5461 ggcacctggc ggccctccca gccccagccg ccgtcgggga cctcgtgaac gatgacgtag
     5521 tggacgccgt cggtgcccgc ggcctccgcg gccgcggtgg cgacacgctg gatcatctcg
     5581 tgcttgcggg ccatggtcgt ccagccgacc ggtgtggcga tctcgacgat cgggcgcgag
     5641 gtgccggccg ccagcggctt gccgcgctcg ccccagccgt cgcgggcgat ctcgtggatc
     5701 aacacccagg tcatctcgtc gaccgggacg ccctcggcct cggccgcggc gcgggtgacg
     5761 cgctcgatca ggacgcgctt gtcgtcgggt gtgaggctgg agccagtgcg ggccagggcg
     5821 gcggttcagc ggacgcgctc gatcacggtt gcgatgccct ggccgccgcc gatgcacagc
     5881 gcgatcacgg cgagctggcg atcgcggcgc tcgagctcgt cgagcgcggt gccgagcagc
     5941 atcgcgccgg tcgcgccgag cgggtggccg agcgcgatcg cgccgccgtt gacgttgacc
     6001 cgctcgggat cgatctcgag cgcgcggatg gtctgcagga cgaccgccgc gaacgcctcg
     6061 ttgatctccc acaggtcgat atcggccggg gtgaggccgg cgatcgcgag cgccttgcgc
     6121 gcggcgggcg ccggcccggt cagcatgatg accggctcgg agccgaccga ggcgaacgcg
     6181 cggatccggg cgcgcgggcg gatgccgctg
//
LOCUS       DASVOR010001441         2420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_423239,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001441 DASVOR010000000
VERSION     DASVOR010001441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423239"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2420
                     /locus_tag="VF516_27335"
     CDS             <1..>2420
                     /locus_tag="VF516_27335"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8111486.1"
                     /translation="TQPGPRTGEVIDRAARLIAHGQAARGGGSPIALDEVSAGLLDAR
                     FDVVEGDTGPMLLGERAVIQGARTLLGRPTSCVGRDWELGALAGILDECIDESSARAA
                     IVTAAAGMGKSRLRAEFVARTRQRCPEAAIWVGRGDSLRAGSTLDLLAQALRGALGIH
                     GGEPLAERHDQIRTRVTEHIPAADHNRIIEFLGELVGAPFPDDRASGALRTARQDAQL
                     MSEQMRKAWLDFLDAETSAHPVLLVLEDLHWGDFGTMQFIDTALRDRAAQPWMVLALA
                     RPEVFDVFPRLWADRRSVQTIRLKELGRKASERLVRQVLGDSVGPETIERLVHQADGN
                     AFYLEELIRAAAEGKDRALPETVLAMVETRLARLAAEARRVLRAASVFGELCWESGVA
                     ALLGGAMSGAMVGEWLTQLVGQEVLTMRPSSRFSGERELAFRHALLREGAYATLTEDD
                     RRLAHGLAGDWLESHGETDPMVLASHFERGGDGVRAARHYKRAVEQAVYVLDLDAAMI
                     RADLGVRCASTPELRIELLSARCEASNYAAQLHPVVMPDAEELMRSAPPGSIPWTQGV
                     QSYILGAMATGRIDDFLATIPLLREVDPAPEAVARMTLACLATICYLDILGQLAEGTA
                     LADRLSAVVRATGDREPLARFWWHILVSMRATYAYDDPWDGLVHADAIQPIFDVIGGE
                     PVFLNMQVFRGMNLWSLGAFEPGRRILADVAAADTSLGPASSLRRFCLSWLLADTGAL
                     DAARALATQLAEYGHAHRIPLEEYRGRWVLAEVLRRTGDLEGAERELDIALGMATPLE
                     RPGVLATL"
BASE COUNT          309 a          894 c          891 g          326 t
ORIGIN      
        1 cgacgcagcc cgggccgcgc acaggcgagg tgatcgaccg cgccgcgcgc ctgatcgcgc
       61 atggccaggc cgcgcgcgga gggggatcgc cgatcgcgct cgacgaggtg agcgccggct
      121 tgctcgacgc ccggttcgac gtggtcgagg gcgacaccgg gccgatgctg ctcggcgagc
      181 gcgccgtgat ccagggcgcg cggaccctgc tcggccggcc gacctcgtgc gtcggccgcg
      241 actgggagct cggggcgctc gccggcatcc tcgacgagtg catcgacgaa tcgagcgcgc
      301 gcgccgcgat cgtgaccgcg gccgccggca tgggcaagtc ccgcctccgc gccgagttcg
      361 tcgcccggac ccggcaacgc tgccccgagg ctgcgatctg ggtcggccgt ggcgactcgc
      421 tgcgcgccgg ctcgaccctc gatctgctcg cccaggcgct gcgcggcgcc ctggggatcc
      481 acggcggcga gccgctcgcc gagcgccacg accagatccg gacccgggtc accgagcaca
      541 tccccgcggc cgatcacaac cggatcatcg agttcctggg cgagctggtc ggcgcgccgt
      601 tccccgacga ccgcgcgagc ggagcgctgc ggacggcgcg ccaggacgcg cagctcatga
      661 gcgagcagat gcgcaaggcg tggctggact tcctcgacgc cgagacctcc gcccacccgg
      721 tcttgctcgt gctcgaggac ctgcactggg gcgacttcgg caccatgcag ttcatcgaca
      781 ccgcgctgcg cgaccgcgct gcgcagccct ggatggtgct cgcgctggcc cgccccgagg
      841 tgttcgacgt gttcccccgg ctgtgggcgg accgccggag cgtccagacg atccggctca
      901 aggagctggg tcgcaaggcc agcgagcggc tcgtccgcca ggtgctcggc gacagcgtcg
      961 gccccgagac gatcgagcgg ctggtccatc aggccgacgg caacgcgttc tacctcgagg
     1021 agctgatccg cgcggccgcc gagggcaagg accgcgcgct ccccgagacc gtgctggcca
     1081 tggtcgagac ccggctcgcc cggctcgcgg ccgaggcccg gcgcgtgctg cgcgccgcca
     1141 gcgtgttcgg cgagctgtgc tgggagagcg gcgtggccgc cctcctgggc ggggccatga
     1201 gtggcgcgat ggtgggcgag tggctcaccc agctggtcgg gcaggaggtg ctgacgatgc
     1261 ggccgagcag ccggttctcc ggggagcgcg agctcgcgtt ccggcacgcg ctgcttcgcg
     1321 agggcgcgta tgcgaccctg accgaggacg acaggcggct cgcgcacggc ctcgccggcg
     1381 attggctcga atcccacggc gagaccgatc ccatggtgct ggcgagccac ttcgagcgcg
     1441 gcggcgacgg cgtccgcgcc gcgcggcact acaagcgcgc cgtcgagcag gcggtgtacg
     1501 tgctcgatct ggatgccgcc atgatccgcg cggacctcgg cgtccgctgc gcgtcgacgc
     1561 ccgagctccg gatcgagctg ctcagcgcgc gctgcgaggc ctcgaactac gcggcgcagc
     1621 tccaccccgt cgtgatgccc gacgccgagg agctcatgcg ctcggcgcca cccggctcga
     1681 tcccatggac gcagggagtg cagtcgtaca tcctcggcgc gatggcgacc ggccggatcg
     1741 acgatttcct ggcgaccatc ccgctgctcc gcgaggtcga cccggccccc gaggccgtcg
     1801 cgaggatgac gctcgcgtgc ctcgcgacga tctgttacct cgacatcctc ggccagctcg
     1861 cggaggggac cgcactggcg gaccggctct cggcggtcgt ccgggcgacc ggggatcgcg
     1921 agccgctcgc ccggttctgg tggcacatcc tggtcagcat gcgcgcgacc tacgcgtacg
     1981 acgacccctg ggacggcctg gtccacgccg acgcgatcca gccgatcttc gacgtcatcg
     2041 gcggcgaacc ggtgttcctg aacatgcagg tgtttcgcgg gatgaacctg tggtcgctgg
     2101 gcgcgttcga accggggcgg cgcatcctcg ccgacgtcgc cgccgccgat acgtcgctcg
     2161 gccccgcgag ctcgctgcgg cggttctgcc tgtcctggct gctcgccgac accggcgcgc
     2221 tcgacgcggc gcgcgcgctg gcgacccagc tcgcggagta cggacacgcg catcgcatcc
     2281 cgctcgagga ataccgcggc cggtgggtgc tggccgaggt gctgcgccgc accggcgacc
     2341 tcgagggtgc cgagcgcgag ctcgacatcg cgctggggat ggcgacgccg ctcgagcgcc
     2401 ccggcgtgct ggccacgctc
//
LOCUS       DASVOR010001442         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_394039,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001442 DASVOR010000000
VERSION     DASVOR010001442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394039"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(151..>1602)
                     /locus_tag="VF516_27340"
     CDS             complement(151..>1602)
                     /locus_tag="VF516_27340"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8111487.1"
                     /translation="VHVIAVGGAPGVARELKRSAPFWQLRRRFGFHHVDARGSVERVT
                     GQRLAWLNQAVEIAARVQAPGDEYFAPRLAQGQQVHGEESRLDTALGNRFPWMTLTLG
                     AVCVVLFVLGKIWSDGNFSLVLYRMGANSGEEVRAGEVWRLVASMFLHGNLEHIAVNM
                     IALAVFGPVLERLLGPQRYLWLYGLSGLGGSLASALLRGPGISVGASGAIWGLMAAGL
                     GLALRPRGLLPPLRLAQARRRAIAPLAINLVYSFQPGVDILAHLGGGVVGFALMASGL
                     ITRGVDPLWTEGAEVRRRRRSSGNLTLATLVFAILLAASVVLALVAGRPWQIGAPPVL
                     ERVRVADTGLVVAVPSTVAKSPQAETKGDVRVFDYGNLQSTPVVVEIVVNPLSQTVPA
                     DQLDEVLENERQAMQDTALPGAQRQGDARIVTVGARRFAFLTHAFKNGVKVRTWTSVL
                     RDLEVVLRVYSTPDRPAAWAGIEDKIVESLQRP"
BASE COUNT          256 a          617 c          542 g          187 t
ORIGIN      
        1 cacgccaccc acgccgcgag cgcgccgccg ccgaccagcg gcgcgaccac cgccggcacc
       61 gaccgcgcgc ggcgatcgtg cagctcggac caccggatca tcgcgcgcga gttcatcgcg
      121 cacagcatac cgcgcaggcc gcggctcagc tcatggccgc tgcaagctct cgacgatctt
      181 gtcctcgatc ccggcccacg ccgcggggcg atccggggtc gagtagacgc gcagcaccac
      241 ctcgagatcg cgaagcaccg aggtccacgt ccgcaccttg accccgttct tgaacgcgtg
      301 ggtgaggaac gcgaaccggc gcgcgcccac ggtcacgatg cgggcgtcgc cctggcgctg
      361 cgctcccgga agcgccgtgt cctgcatcgc ctgtcgctcg ttctccagca cctcgtcgag
      421 ctgatcggcc ggcacggtct gcgacagcgg gttgaccacg atctcgacca cgaccggcgt
      481 gctctgcagg ttgccgtagt cgaacacgcg cacatcgccc ttggtctcgg cctgcggaga
      541 cttcgcgacg gtgctgggga ccgccaccac caggccggtg tcggcgacgc gcacgcgctc
      601 gagcaccggc ggggcgccga tctgccaggg ccgtccggcg accagcgcca gcaccaccga
      661 ggccgccagg aggatggcga acaccagcgt cgccagcgtc aggttgcccg acgagcgccg
      721 ccgccgccgg acctcggcgc cctcggtcca cagcggatcg acgccgcgcg tgatcagccc
      781 cgacgccatg agcgcgaagc ccacgacgcc gccgccgaga tgggccagga tgtcgacccc
      841 gggctggaag gagtacacca ggttgatcgc caggggagcg atggcgcggc ggcgcgcctg
      901 cgccaggcgc agcggcggca acagcccgcg aggtcggagc gcgagaccga gcccggccgc
      961 catgagaccc cagatcgccc ccgacgcacc caccgagatg cccgggccgc gcagcagcgc
     1021 gctggccagg ctgccgccca gccccgacag gccgtacagc cacaggtagc gctgcggtcc
     1081 caggagccgc tcgagcaccg ggccaaacac ggcgagcgcg atcatgttga ccgcgatgtg
     1141 ctccaggttg ccgtggagga acatcgacgc taccaggcgc cacacctctc ccgcgcggac
     1201 ctcctcgccg ctgttggcgc ccatgcggta cagcacgagc gagaagttgc cgtccgacca
     1261 gatcttgccg agcacgaaca ggaccacgca gacggcgccc agcgtcagcg tcatccaggg
     1321 gaagcggttg cccagggcgg tgtcgagccg gctctcctcg ccatggacct gctggccctg
     1381 cgcgaggcgc ggcgcgaagt actcgtcgcc gggagcttgc accctggcgg cgatctcgac
     1441 cgcctggttc agccaggcga gccgctgccc cgtgacgcgc tcgacgctgc cgcgggcgtc
     1501 gacgtggtga aatccgaatc gccgccgcaa ttgccagaac ggagcgctcc gcttcagctc
     1561 gcgcgcgacc ccaggggcgc cgcccacggc gatcacgtgc ac
//
LOCUS       DASVOR010001443         5344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_190394,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001443 DASVOR010000000
VERSION     DASVOR010001443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5344
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190394"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VF516_27345"
     CDS             <1..631
                     /locus_tag="VF516_27345"
                     /inference="COORDINATES: protein motif:HMM:NF041940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choice-of-anchor X domain-containing protein"
                     /protein_id="HEX8111488.1"
                     /translation="GQDHTQPGPGERTIAESLSAELKFPEAVVLGQPFAVGARWTYQR
                     TTTAREYQYAANDVHRNVHVLSKYEITAPEVNRAYAEEAWIVRARFWDAQGKGMTGAE
                     LFVQCFLIGPCAQVIRVLLQDDGVAPDAVAGDGTYTALHRFTREAEGLWKYLVIAQDV
                     NTARPDMTPEQAARVVGGIVRTHQLTISFGSGTCRLIPDGYIHVIGGLT"
     gene            785..2101
                     /locus_tag="VF516_27350"
     CDS             785..2101
                     /locus_tag="VF516_27350"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8111489.1"
                     /translation="MVGRLGRALKHRNYRLYFAGQGISVIGTWLTRFATVWMAYDLTG
                     SALVLGLVGFFGQAPTSILAPFAGVMVDRWNRHRTIIVTQIAAMLQSAALAFFAWTHT
                     MTVWHLIVLGAVQAVINAFDMPSRQSFLGQMIEDRADLPTAIALNSSMVNAARLIGPV
                     IAAGLVAAWGAEGCFTIDAASYLAVIGSLLAMRVRPRPAPARSRRVLADLAEGLRYVW
                     HFPLIRAVLLLLASSSVLGGAYSTLLPVIAATTLHGGPHTLGILMGSAGCGALAGALY
                     LASRTSVVGLVAVINRCALGLGAGLVAMELASRVAIAVPLLFVIGMSMMMQMASTNTL
                     IQSIVDDKMLGRVISLYAVAFFGGAPIGSLLEGTLASQIGAIHTFAVAGVLCLAAGAV
                     FAWKLPGLRRVSQPHYVKLGLIPDPAAPHDAKGPGAARATHRAEAD"
     gene            2558..3898
                     /locus_tag="VF516_27355"
     CDS             2558..3898
                     /locus_tag="VF516_27355"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8111490.1"
                     /translation="MFARNWKRMFALGAGALLFGCSPSAVDDPDLASTTAEATVVGGH
                     LAGIGDADFSAAQANFNQVETINDGVGPIFNDVACGRCHSLGAIGGAGQQIERRFGKF
                     VNGVFNSLASEGGSLRQLQSVGSFTGASGQACNVPVEHEPTDATVHNVGRLTTSLFGL
                     GLVDAMPDSFFTNLVAAEPATVRGTVNTAAIALPNPADASQSIGKVRVARFGWKADTA
                     NLADFAAGAYLNEMGITTQHCFKGTSITAFTAESRPNGVAVPAGCDDLAPPAPAGVPA
                     GTDDAVGPCAAGQTEIQDDVQNFLTFMTFLAPAPRDLSDLISINAGAPVFNQIGCNGC
                     HVTTTFTTPNPAPNGVPGGFAFNPFSDFAVHDMGTLGDQIGKDGDSVARTRQMRTAPL
                     WGIRFRNNLLHDGRTTDIPTAIAAHDGQGKAAATAFAALSATDQHNVVQFIRSL"
     gene            3976..4602
                     /locus_tag="VF516_27360"
     CDS             3976..4602
                     /locus_tag="VF516_27360"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8111491.1"
                     /translation="MSLTFYYAPMSTASVTELVLDELGVPCDRVKLDLKKGDTKKPEY
                     LAVNPNGKVPCIVHDGTAIWESAALTIYLGETFGVERGLFPTPGPRRGEAMKWIVWTN
                     VTLGDAVARFARNTMDWIPAEQRNAKAGEAGKADIADCLRIVDQALAGKQYLLGDYTL
                     ADTHLTSFCDWLRYMQIDFTPFKNVQAWGERCRARPAYQKLMAAMTGG"
     gene            4682..>5344
                     /locus_tag="VF516_27365"
     CDS             4682..>5344
                     /locus_tag="VF516_27365"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX8111492.1"
                     /translation="MVVGFTRCARPELGRVSTYTVLGFAGYAAASVLAAVLFVAWDLP
                     AGDRLVGAFAPPLAFLAVVAVVRTLVGGERIVFYQTATSGVVAVAVADALAGGHVARI
                     VDVAVLGIGTFLVFGRFGCFAVACCHGRPARFGVVYGPDHVRVGLWARWSGRPLWPVQ
                     LVECVASLALVIAGLVAGWDEPGLPAVIYIAGYAAVRFLLELRRGDAERPQRLGASEA
                     QWT"
BASE COUNT          747 a         1898 c         1881 g          818 t
ORIGIN      
        1 cggccaggac cacacgcagc ccggcccggg cgagcggacg atcgcagaga gcctgtccgc
       61 cgagctgaag ttccccgagg cggtcgtgct cggccagccg ttcgcggtcg gcgcgcgctg
      121 gacgtaccag cggaccacca ccgcgcgcga gtaccagtac gccgcgaacg acgtgcaccg
      181 gaacgtgcac gtgctctcga agtacgagat caccgcaccc gaggtcaacc gcgcgtatgc
      241 ggaagaagcc tggatcgtcc gggcgcggtt ctgggacgcc cagggcaagg ggatgaccgg
      301 cgccgagctg ttcgtccagt gcttcctcat cggcccgtgc gcgcaggtga tccgggtgct
      361 cctgcaggac gacggcgtgg cgcccgacgc cgtcgccggc gacggcacct acaccgcgct
      421 ccatcgcttc acgcgcgagg ccgaggggct gtggaagtac ctggtcatcg cgcaggacgt
      481 caacaccgcc cggcccgaca tgacgccgga gcaggccgcc cgggtcgtcg gcggcatcgt
      541 gcgcacgcac cagctgacga tctcgttcgg gagcggcacc tgccggctga tccccgatgg
      601 gtacatccac gtcatcggcg ggctgacctg acctggaaca tcggaccgcg gccgccgccg
      661 cgtgcacccc cgcgaccggg ctgcggcatc ccaggtacgg caccccgtgg cttccaccga
      721 tggctccgcg aaggatttcc cacgcccggt gaagccaggt ggcagtcgca cgctaggctt
      781 gggcatggtc ggccggctcg gtcgcgcgct caagcaccgg aactaccggc tgtactttgc
      841 aggccagggg atctcggtga tcggcacctg gctgacccgg ttcgcgacgg tgtggatggc
      901 gtacgacctg accggctcgg cgctcgtgct cggcctcgtc gggttcttcg gacaggcgcc
      961 gacgtcgatc ctcgcaccgt tcgccggggt gatggtcgac cgctggaacc gccaccggac
     1021 gatcatcgtc acgcagatcg cggcgatgct gcagtcggcg gcgctcgcgt tcttcgcgtg
     1081 gacccacaca atgacggtgt ggcacctgat cgtgctcggc gcggtgcagg cggtgatcaa
     1141 cgcgttcgac atgccatcgc gccagtcgtt cctcggccag atgatcgagg accgcgccga
     1201 cctgcccacc gcgatcgcgc tgaactcgtc gatggtcaac gccgcgcggc tgatcggccc
     1261 ggtcatcgcg gccgggctgg tcgccgcgtg gggcgccgag ggctgcttca cgatcgacgc
     1321 ggcgagctac ctcgcggtga tcggatcgct gctggcgatg cgggtccggc cgcggccggc
     1381 gccggcgcgg tcccgccgcg tgctggcgga cctcgccgag ggcctgcgct acgtctggca
     1441 cttcccgctg atccgcgcgg tgctgctgct gctcgcgtcg tcgagcgtgc tcggcggtgc
     1501 gtactcgacg ctgctgccgg tgatcgcggc caccacgctg cacggcggcc cgcacaccct
     1561 cggtatcctg atgggatcgg ccgggtgcgg cgcgctggcc ggcgcgctgt acctggcgag
     1621 ccgcacgtcg gtggtcggcc tggtggcggt gatcaaccgc tgcgcgctcg gcctgggcgc
     1681 cgggttggtc gcgatggagc tggcgtcgcg ggtcgcgatc gcggtgccgc tgctgttcgt
     1741 gatcggcatg tcgatgatga tgcagatggc ctcgaccaac acgctgatcc agtcgatcgt
     1801 cgacgacaag atgctcggcc gggtgatcag cctgtacgcg gtggcgttct tcggcggcgc
     1861 cccgatcggc tcgctgctcg agggcacgct ggcgagccag atcggcgcga tccatacgtt
     1921 cgcggtcgcc ggcgtgctgt gcctcgcggc cggcgcggtg ttcgcgtgga agctgcccgg
     1981 gctgcgccgg gtcagccagc cgcactacgt caagctcggc ctgatcccgg atccggcggc
     2041 gccgcacgac gccaagggcc ccggcgcggc gcgcgcgacg catcgcgctg aagcggactg
     2101 atccgggcgc acccgggccc gtgcgatcag cgcgggctcg agaaatcgct cggttcctgg
     2161 ctcgctggtg cgcggcgcgc atcagatcac ggcgcgtagc gcgacgcgtc acccgcagcg
     2221 gttcgtgaga tgaggccacg agtcgaactc gcttcgtgag attccggatc tgtcacgcaa
     2281 tggaccgtcg ggtgtccgcg cctcgacgtg aggcgatcac cacgatgcga gagaatccag
     2341 attgcgatgt tcgtcatggg gtgtcgcgcc accgtcatca tgaaatcgcc gcaccgcgat
     2401 cgagatcatc agacatcgat ttcgcgattg acaggatgtg cagtggaggc atagggtcga
     2461 tccactttgg gaacctatgg gttcctgaac agccaccggg tccggccccg tgaggctcgg
     2521 ccccctatca tctctggagg tttcattgat gacgcgtgtg ttcgctcgca attggaagag
     2581 gatgttcgcg ctcggcgccg gcgcgctgct attcgggtgc tcgccgtcgg cggtcgatga
     2641 tccggacctg gcatcgacca ccgccgaggc caccgtggtg ggcggccacc tcgccgggat
     2701 cggcgacgcc gacttcagcg cggcgcaggc caacttcaac caggtcgaga ccatcaacga
     2761 cggcgtcggg ccgatcttca acgacgtcgc ctgcgggcgc tgtcactcgc tcggcgcgat
     2821 cggcggcgcg ggccagcaga tcgagcggcg gttcggcaag ttcgtcaacg gcgtgttcaa
     2881 ctcgctcgcc agcgagggcg gatcgctgcg ccagctgcag tcggtcggat ccttcacggg
     2941 cgccagcggc caggcctgca acgtcccggt ggagcacgag ccgaccgacg ccaccgtgca
     3001 caacgtcggc cggctcacga cgtcgctgtt cgggctcggc ctggtcgacg cgatgcccga
     3061 ctcgttcttc acgaacctgg tcgccgccga gccggccacc gtgcgcggca ccgtcaacac
     3121 ggcggcgatc gcgctgccca acccggccga cgcgtcgcag agcatcggca aggtccgggt
     3181 cgcgcggttc ggctggaagg ccgacacggc gaacctcgcg gacttcgccg ccggcgccta
     3241 cctgaacgag atggggatca ccacccagca ctgcttcaag ggcacgtcga tcaccgcgtt
     3301 caccgccgag tccaggccca acggcgtcgc ggtgccggcc ggctgcgatg acctcgcgcc
     3361 cccggccccg gccggcgtcc cggcggggac cgacgacgcc gtcggcccgt gcgcggccgg
     3421 tcagaccgag atccaggacg acgtgcagaa cttcctcacg ttcatgacgt tcctcgcgcc
     3481 cgcgccgcgc gacctgagcg acctgatctc gatcaacgcc ggggcgccgg tgttcaacca
     3541 gatcggctgc aacggctgcc acgtgaccac gacgttcacc acgccgaacc cggcgcccaa
     3601 cggcgtcccc ggcggcttcg cgttcaaccc gttctccgac ttcgcggtcc acgacatggg
     3661 cacgctgggc gaccagatcg gcaaggacgg cgacagcgtg gcgcggactc gccagatgcg
     3721 caccgcgccg ctgtggggca tccggttccg caacaacctg ctccacgacg ggcggacgac
     3781 ggacatcccg accgcgatcg cggcgcacga cggtcagggc aaggcggccg ccaccgcgtt
     3841 cgccgcgctg agcgcgaccg atcagcacaa cgtcgtgcag ttcatccggt cgctgtgacc
     3901 ccgggcggtt gacgaccggg cctccgtcgc ccaccatccg cgagccggac ttgaccggcg
     3961 tccacggaga gcgagatgag cctgacgttc tactacgcac cgatgtccac cgcgtccgtc
     4021 accgagctgg tcctcgatga gctcggggta ccgtgcgacc gggtgaagct ggatctcaag
     4081 aagggcgaca ccaagaagcc cgagtacctc gcggtgaacc ccaacggcaa ggtgccgtgc
     4141 atcgtccacg acggcaccgc gatctgggag tcggcggcgc tgacgatcta cctcggcgag
     4201 acgttcggcg tcgagcgcgg gctgttcccg accccgggcc cccggcgcgg cgaggcgatg
     4261 aagtggatcg tgtggaccaa cgtcacgctg ggcgacgcgg tggcgcggtt cgcgcgcaac
     4321 accatggact ggatccccgc cgagcagcgc aacgccaagg ccggcgaggc cggcaaggcc
     4381 gacatcgccg actgcctgcg catcgtcgat caggcgctcg ccggcaagca gtacctgctc
     4441 ggcgactaca cgctggccga tacccacctg acgtcgttct gcgactggct gcgctacatg
     4501 cagatcgact tcacgccgtt caagaacgtc caggcctggg gcgagcgctg tcgcgcgcgg
     4561 ccggcgtacc agaagctgat ggcggcgatg accggcggct gacccacccg gtgattcatg
     4621 gcgggcatgc cgccgggcgt cgacgagccc ccggtgatgg tatccggcgt aggataccgg
     4681 cgtggtggtc ggtttcacgc ggtgtgcccg cccggagctc gggcgggtca gcacgtacac
     4741 cgtgctgggg ttcgccggct atgccgcggc gtcggtcctg gccgccgtcc tgttcgtggc
     4801 ctgggacctg ccggccggcg accgcctggt cggggcgttc gccccgccgc tggcgttcct
     4861 cgcggtggtc gcagtggtcc gcaccctcgt gggaggcgag cgcatcgtgt tctaccagac
     4921 cgccacctcc ggggtcgtcg cggtcgcggt cgccgacgcg ctcgccggcg gccacgtcgc
     4981 ccgcatcgtc gacgtcgccg tgctcggcat cggcacgttc ctcgtgttcg gccggttcgg
     5041 ctgcttcgcg gtcgcgtgct gccacggccg gccggcgcgg ttcggcgtgg tctacggccc
     5101 cgaccacgtc cgcgtcgggc tgtgggcccg gtggagcggc cggccgctgt ggccggtcca
     5161 gctcgtcgag tgcgtcgcga gcctcgcgct cgtgatcgcc gggctggtcg ccggctggga
     5221 cgagcccggc ctgcccgccg tcatctacat cgccggctac gcggccgttc gcttcctgct
     5281 cgagctccgc cgcggcgacg ccgagcgccc gcagcgcctc ggcgccagcg aggcgcagtg
     5341 gacc
//
LOCUS       DASVOR010001444         7074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_365484,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001444 DASVOR010000000
VERSION     DASVOR010001444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7074
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365484"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..409
                     /locus_tag="VF516_27370"
     CDS             <1..409
                     /locus_tag="VF516_27370"
                     /inference="COORDINATES: protein motif:HMM:NF022073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0047989 - hydroxybutyrate-dimer hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019605 - butyrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-hydroxybutyrate oligomer hydrolase family
                     protein"
                     /protein_id="HEX8111493.1"
                     /translation="RVAADPAIAQRIAQVASTGRIRRPLITVAGTMDALLPIQRGARA
                     YEALVRHGDRDDRGRPAYRLYEVQNGNHIETFRKTFPQLELIQPHAQRAFDLLVDHVE
                     HHASLPPSQCIPRGGAITSGPAQPGHCAQLLAP"
     gene            549..2246
                     /locus_tag="VF516_27375"
     CDS             549..2246
                     /locus_tag="VF516_27375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEX8111494.1"
                     /translation="MSSTSQGVTLTQVNNFGSNPGALQMFTFVPSGLPSGAPLVVVLH
                     GCTESASSYGAQTEWGNLGQRFGFATVFAQQQTANNSSSCFNWFQTGDITRGQGEALS
                     IKQMVDTMKSTVGSDPARIFVTGISAGGLMTTVMMATYPDVFAGGAVMSGGPYMCATS
                     LTGANTCQMGNVSHTPQQWGDLVRNAVPSYHGPYPRLISFHGSSDTTVAPADQQQQVD
                     QWSNVLGIDETADVNETFRTATHKVYRDASGRSMIETYLVSGMGHALTVDVGTNVDQG
                     GQTGAFSEDHDIYSSFYSAQFFGLTGGGGGGGGDTTPPTVSITAPAAGATVSGTVNVA
                     ANASDNVGVTRVDVLVDGTVAGSDTTAPYSVAVDTTRLANGSHTLTARAFDAANNSAT
                     SAAVAVTVQNAGGGGGTHLETFSSSSGPDNPGWTLGGWTLSTKDATGTAGSRSITATA
                     APSFNTVTKTATWANLALGSAPRLSYQRQLALSDANITASAGFSVIINDGSDHVVDSK
                     TISGFTSYNESAFTARTNLDLSAFAGKTVTLKLVVTATDSSSTVTSATAVVDQIQIQ"
     gene            2709..4082
                     /locus_tag="VF516_27380"
     CDS             2709..4082
                     /locus_tag="VF516_27380"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX8111495.1"
                     /translation="MAAALSVSGNVLAQTPPDPAQPTTPPSDQPGTTATPANPPAPGP
                     PPVAEQPPNVAPPAPPPTSFPTMTGPALRLSDLFTFRPGVFIQLWGSFVQDVNKQTNG
                     DAGDFARNIYLRRARFFFGGTIGKSLSYFILAESANNGSPTADAMGNINKNVTTFALN
                     DAYLDFKVNDLLSIQAGLMLIPFTRNILQSTSTYWTLDVGAVSATYIAATETTVLRDY
                     GAQVKINAADNHFELRGMASEGVRIADLTPSAATPPAPVARNPGKNNPRLTAFAQYNF
                     LDPDTGYVFNGQYFGRKKIAGVAAGVDYQPISSDNPYFATSLTGFAAIPVHGGDAKNG
                     DDEFGGQIEYLHFHGGGVVPGSPASGLAKQNALLVEAGYYNKGAHLSVFGKFEGRFFD
                     TPDGSPLKAGNSRVYGAGVKYFLAEQIANLTLMYSLTQAPDLPAPVAALRNDSNAIQI
                     QLQVGYF"
     gene            4212..5804
                     /locus_tag="VF516_27385"
     CDS             4212..5804
                     /locus_tag="VF516_27385"
                     /inference="COORDINATES: protein motif:HMM:NF004837.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8111496.1"
                     /translation="MPTQLTSWLLFQNAATHFGEVEIVSKEATGTHRYSYRAFTERAQ
                     QLMHALDALGIARGERIATLAWNHYRHLELYFAVPCTERVLHTLNLRLSLDDLSYIIE
                     HADDRAIFVDPDQVATLEKLGPALSRVAHIVVLGPEVPPSSLPNLIAYEDLIKPHPTD
                     YPPRDIAEATPLGICYTSGTTGRPKGVVYTHRSTFLHSLAVTSAAGNAFGPGDAILPV
                     VPMFHANAWGIPHAGIMVGAKLVFAGRDVSPPSLVDLLAQERVTLSGGVPTVWLGVGD
                     ELARRGTRLPDLKRVLCGGSQPPTSLIERYRTEFGVLIQQAWGMTETSPLASIAWPKE
                     YMRDWPADRVTQAVRTQAGLPLPGIQISIRGMDGNEVPRDGKTMGDLLVRGPWVVDSY
                     YKGEGKEQFTADGWFKTGDMAIASPEGYFVIGDRTKDLIKSGGEWISSVDMERAIMAM
                     PEVAEAAVVAVPDAKWGERPLACIVIRAGATLGLAAVRDHLRANGFATWQLPDRIELI
                     EAVPKTSVGKFDKKVLRARYAR"
     gene            5801..>7074
                     /locus_tag="VF516_27390"
     CDS             5801..>7074
                     /locus_tag="VF516_27390"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8111497.1"
                     /translation="MSSRRSLLPGDAEQDVTSRVAPAHTIRIWSVVLGVVDGPSRGAR
                     AQVGSEIARIGTADGNDLVLVDRTVSRFHCEVSVRGNTIVIRDCGSTNGTLIDGVRVR
                     EAEIPPGTLVRIGGSALRVELGEEPAFVEVSGRSSFGELVGGSVEIRRVYAILERLAQ
                     TDATVLVQGETGTGKDVLARSLHAASRRADRPFVAVDCGAIPEHLVESELFGHVKGAF
                     TGATSDRRGVFEEADGGTLFLDEIGEMPLALQPKLLRAIESRSIRRVGGTGARTVDVR
                     IVAATNRSLASSINDGSFREDLYYRLAVVEVRLPPLRARRDDIPVLATHFFRRFAGDG
                     VLPAEFVAGLVGRGWPGNVRELRNYIERSVSLGTIAPPHAAAPATAPPPAGPVPPDSI
                     ERFVALHLPLKEARRAWTESFELVYVRAMLKLV"
BASE COUNT         1096 a         2585 c         2380 g         1013 t
ORIGIN      
        1 ccgggtcgcg gccgacccgg cgatcgccca gcgcatcgcc caggtcgcgt ccaccgggcg
       61 gatccggcgg ccgctgatca ccgtggccgg cacgatggac gcgctgctgc cgatccagcg
      121 cggcgcgcgg gcctacgagg ccctggtccg gcacggcgac cgcgacgacc gcggccggcc
      181 ggcgtaccgg ctgtacgagg tccagaacgg caaccacatc gagaccttcc ggaagacgtt
      241 cccgcagctc gagctgatcc agccccacgc gcagcgcgcg ttcgatctgc tggtcgacca
      301 cgtcgagcac cacgcatcgt tgccgcccag ccagtgcatc ccgcgcggcg gggcgatcac
      361 cagcgggccg gcgcagcccg ggcactgcgc acagctgctg gctccctagg ctttcgagac
      421 tcctttcgag ccacctaccc ccccccccgc ccgcggagtg agccatgaac cgagccgtgt
      481 ctggttgttc ggtcgttgct gtcctgtccc ttgcatcggc gtgcgcggtc gcaccgccgg
      541 atccgctggt gtcgagcacc tcccaggggg tcacgctgac ccaggtcaac aacttcggca
      601 gtaaccccgg ggcgctgcag atgttcacgt tcgtgccgtc cgggctgccg tcgggcgcgc
      661 cgctggtcgt ggtgctgcac ggctgcaccg agagcgcgag ctcgtacggg gcccagaccg
      721 agtggggcaa cctcggccag cgcttcgggt tcgcgacggt gttcgcccag cagcagaccg
      781 cgaacaactc gtcgtcctgc ttcaactggt tccagaccgg cgacatcacg cgcggccagg
      841 gcgaggcgct gtcgatcaag cagatggtcg acaccatgaa gtcgacggtc ggcagcgatc
      901 cggcgaggat cttcgtcacc gggatctcgg cgggcggcct gatgaccacg gtgatgatgg
      961 cgacctaccc cgacgtgttc gccggcggcg cggtcatgtc gggcggcccg tacatgtgcg
     1021 cgaccagctt gaccggcgcg aacacctgcc agatgggcaa cgtcagccac accccgcagc
     1081 agtggggcga tctggtacgc aacgcggtcc cgagctacca cggcccgtac ccgcggctga
     1141 tctcgttcca cggctcgtcg gacaccacgg tggcgccggc cgaccagcag cagcaggtcg
     1201 accagtggag caacgtgctc gggatcgacg agaccgccga tgtcaacgag acgttccgga
     1261 ccgcgaccca caaggtctac cgcgacgcga gcggccgctc gatgatcgag acctacctgg
     1321 tcagcggcat gggccacgcg ctcaccgtcg acgtcggcac caacgtcgac cagggcggcc
     1381 agaccggcgc gttctcggag gaccacgaca tctactcgtc gttctactcc gcccagttct
     1441 tcgggctgac cgggggtggc ggcgggggcg gcggcgacac caccccgccg acggtctcga
     1501 tcaccgcccc ggcggccggc gcgacggtca gcggcacggt caacgtggcg gcgaacgcga
     1561 gcgacaacgt cggggtgacc cgggtcgacg tgctggtcga cggcaccgtc gccggcagcg
     1621 acaccaccgc gccgtactcg gtcgcggtcg acacgacgcg gctcgccaac ggcagccaca
     1681 cgctgaccgc ccgggcgttc gacgccgcga acaactcggc gacctcggcc gcggtcgccg
     1741 tcacggtcca gaatgccggc ggcgggggcg gcacccacct cgagacgttc tccagcagct
     1801 ccggccccga caacccgggc tggacgctgg gcggctggac gctgtcgacc aaggacgcga
     1861 ccgggaccgc gggctcgcgg tcgatcacgg cgaccgccgc cccgagcttc aacacggtga
     1921 ccaagaccgc gacctgggcc aacctcgcgc tcggctcggc gccccggctc agctaccagc
     1981 gccagctcgc gctgtcagac gccaacatca ccgcgagcgc gggcttcagc gtgatcatca
     2041 acgacggcag cgatcacgtg gtcgattcga aaacgatctc cggattcacc tcgtacaacg
     2101 agtccgcctt caccgcccgg accaacctcg atctgtccgc cttcgccggc aagacggtca
     2161 cgctcaagct ggtggtcacc gccaccgact cgtcatcgac ggtgaccagc gcgaccgcgg
     2221 tggtcgatca gatccagatc cagtagctcg gcccggaccg gtaacgcgtc gcgctgcgcc
     2281 gcggcgatcc acgtaacgca tcgcgtcgtg ccggagacgg ccgatcggat cgccgcccgt
     2341 cgctcgcagc gcatgcattt ttttggcgcg gcgcgggggc aaagcgcgat atatggcgac
     2401 ctgaacccgc ggcgctgggg tggtttggcg acgcgggcag actggccatt cgatctcgcg
     2461 tccatgaatc cgggtggatt gtacccatca cttcgcgttt ccccggccga actgccaggc
     2521 ggaacgctct cctgctacgt ggaacgcctt cctgcgaggc ggaacacgtt ttttggcggc
     2581 agaagtggac cgatgcgcga atcggcgcga cgatgattcc tcgaaacgac acctcgaatc
     2641 gaacctttcc caacgaggag cacgacgtga ttcagcttgg caaacgaacc cgacgatgcg
     2701 ctctgcttgt ggccgccgcg ttgagcgtct ccggcaacgt gctcgcacag acgccccccg
     2761 atccggcgca gcccaccacg ccgcccagcg atcagccggg cacgacagcg accccggcca
     2821 atccgcccgc gccgggtcca ccgccggtcg cggagcagcc gcccaacgtc gcgccgcctg
     2881 ccccgccgcc gacgtcgttc ccgacgatga cgggaccggc gctgcggctg agcgacctgt
     2941 tcacgttccg gcccggcgtc ttcatccagc tctggggatc gttcgtgcag gacgtgaaca
     3001 agcagaccaa cggcgatgcc ggcgacttcg cccgcaacat ctacctgcga cgcgcccggt
     3061 tcttcttcgg cggcaccatc ggcaagagcc tgagctactt catcctggcg gagagcgcca
     3121 ataacggctc gccgaccgcc gatgcgatgg gcaacatcaa caagaacgtc accacgtttg
     3181 ccctcaacga cgcatacctc gacttcaagg tcaatgacct gctgtcgatc caggccggcc
     3241 tgatgctgat tccgttcacg cggaacatcc tgcagtcgac ctcgacctac tggacgctcg
     3301 acgtcggagc cgtatccgcg acctacatcg ccgcgaccga gaccacggtg ctgcgcgact
     3361 acggcgccca ggtcaagatc aacgcggccg acaaccactt cgagctgcgc ggcatggcgt
     3421 cggaaggcgt ccggattgcg gacctcacgc cgtcggcggc caccccgccg gcgccggtgg
     3481 cgcgcaaccc cggcaagaac aacccgcggc tcaccgcgtt cgcgcagtac aacttcctcg
     3541 atcccgatac cggctacgtg ttcaacggcc agtacttcgg ccgcaagaag atcgccggtg
     3601 tcgcggcggg cgtggactac cagccgatct ccagcgacaa cccgtacttc gcgacctcgc
     3661 tgaccgggtt cgccgcgatc ccggtccacg gcggcgatgc caagaacggc gacgacgagt
     3721 tcggcggtca gatcgagtac ctgcacttcc acggcggcgg cgtggtcccg gggtcgccgg
     3781 cttcggggct ggccaagcag aacgcgctgc tggtcgaggc cggctactac aacaaggggg
     3841 cccacctgag cgtgttcgga aagttcgagg gtcggttctt cgacaccccg gacggcagcc
     3901 cgctcaaggc cggcaactcg cgcgtgtacg gggccggcgt gaagtacttc ctcgccgagc
     3961 agatcgccaa cctgacgctg atgtacagcc tgacccaggc gcccgacctg ccggcgccgg
     4021 tggccgcgct gcgcaacgac tccaacgcga tccagatcca gctccaggtc ggatacttct
     4081 gatctcgtct ggctcgtttg ggccgtttgg ggcgcgtccg cgcgccgacc tgcggtaaca
     4141 tcccccgcga acccacgccg ccgctcccga cgagcgccgg cggctgtgct tcaggaggta
     4201 ctaccgcgat gatgccgacg cagctcacct cgtggcttct gttccagaac gccgcgaccc
     4261 acttcggcga ggtcgagatc gtcagcaagg aggcgacggg gacgcaccgc tacagctacc
     4321 gggcgttcac cgagcgcgcc cagcagctga tgcacgcgct cgacgcgctc ggcatcgcgc
     4381 gcggcgagcg gatcgcgacg ctggcgtgga accactaccg gcacctcgag ctgtacttcg
     4441 cggtgccgtg caccgagcgc gtgctgcaca cgctcaacct ccggctgtcg ctcgacgacc
     4501 tgtcgtacat catcgagcac gccgacgacc gcgcgatctt cgtcgatccg gatcaggtgg
     4561 cgacgctcga gaagctcggc ccggcgctgt cgcgggtcgc gcacatcgtc gtgctcggac
     4621 ccgaggtccc gccgtcgagc ctgcccaacc tgatcgcgta cgaagatctg atcaagccgc
     4681 acccgaccga ctacccgccg cgcgacatcg ccgaggccac gccgctcggc atctgctaca
     4741 cgtcgggcac gaccgggcgg cccaagggcg tggtctacac ccaccgctcg acgttcctgc
     4801 actcgctcgc ggtgacgtcg gccgcgggca acgcgttcgg cccgggcgac gcgatcctcc
     4861 cggtggtgcc gatgttccac gccaacgcgt ggggcatccc gcacgccggc atcatggtcg
     4921 gcgccaagct ggtgttcgcc ggccgcgacg tcagcccgcc gagcctggtc gatctcctgg
     4981 cgcaggagcg ggtcacgctg tcgggcggtg tgccgacggt gtggctcggc gtcggcgacg
     5041 agctcgcccg gcgcggcacc cggcttcccg acctcaagcg ggtcctgtgc ggcggcagcc
     5101 agccgccgac cagcctgatc gagcgctacc gcaccgagtt cggggtgctg atccagcagg
     5161 cctggggcat gaccgagacc tcgccgctgg cctcgatcgc gtggcccaag gagtacatgc
     5221 gcgactggcc ggcggaccgg gtgacccagg cggtgcggac ccaggccggc ctgccgctgc
     5281 ccggcatcca gatctcgatc cgcgggatgg acggcaacga ggtcccgcgc gacggcaaga
     5341 ccatgggcga cctcctggtc cgcggcccgt gggtggtcga cagctactac aagggcgagg
     5401 gcaaggagca gttcaccgcc gacggctggt tcaagaccgg cgacatggcg atcgcctcgc
     5461 ccgagggcta cttcgtgatc ggcgaccgga ccaaggacct gatcaagtcg ggcggcgagt
     5521 ggatctcgtc ggtcgacatg gagcgcgcga tcatggcgat gcccgaggtc gccgaggccg
     5581 cggtggtcgc ggtccccgac gccaagtggg gcgagcggcc gctggcgtgc atcgtcatcc
     5641 gcgccggcgc aacgctcggg ctcgccgccg tgcgcgacca cctgcgggcg aacgggttcg
     5701 cgacctggca gctgcccgat cgcatcgagc tgatcgaagc cgtgcccaag acctccgtcg
     5761 gcaagttcga caagaaggtg ctgagggcgc gctacgcgcg gtgagctccc ggaggtcgct
     5821 tctgcccggc gacgctgaac aggacgtcac ctcgcgcgtc gcgcccgccc acacgatacg
     5881 gatctggtcg gtcgtgctcg gggtcgtcga tggcccgtcg cgcggcgcgc gcgcccaggt
     5941 cggcagcgag atcgcccgga tcggcaccgc cgacggcaac gatctcgtgc tcgtcgaccg
     6001 cacggtgtcg cggttccact gcgaggtctc ggtccgcggc aacacgatcg tgatccggga
     6061 ctgcggcagc accaacggca cgctgatcga cggcgtccgg gtccgcgagg ccgagatccc
     6121 gcccggcacg ctggtccgga tcggcggctc ggcgctccgg gtcgagctcg gcgaggagcc
     6181 ggcgttcgtc gaggtgtccg gccggtcgtc gttcggcgag ctggtcggcg gcagcgtcga
     6241 gatccgccgg gtctacgcga tcctcgagcg gctggcccag accgacgcga cggtgctggt
     6301 ccagggcgag accgggaccg gcaaggacgt gctcgcgcgc tcgctgcacg ccgcgtcgcg
     6361 ccgcgccgat cggccgttcg tcgcggtcga ctgcggcgcg atccccgagc acctggtcga
     6421 gagcgagctg ttcggccacg tcaagggcgc gttcaccggc gcgacctcgg atcgccgcgg
     6481 cgtgttcgag gaggccgacg gcggcacgct gttcctcgac gagatcggcg agatgccgct
     6541 cgcgctgcag cccaagctgc tgcgcgcgat cgagtcgcga tcgatccgcc gggtcggcgg
     6601 caccggcgcg cgcacggtcg acgtccggat cgtcgcggcg accaaccgct cgctggcgag
     6661 ctcgatcaac gacggcagct tccgcgagga cctgtactac cggctcgcgg tggtcgaggt
     6721 ccggctgccg ccgctgcgcg cccggcgcga cgacatcccg gtgctcgcca cccacttctt
     6781 ccggaggttc gccggggacg gcgtgctgcc cgccgagttc gtcgccgggc tggtcggccg
     6841 gggctggccg ggcaacgtcc gcgagctgcg caactacatc gagcgcagcg tgtcgctcgg
     6901 gacgatcgcg ccgccgcacg cggccgcccc tgccaccgcc ccaccgccgg ccggcccggt
     6961 gccgccggac tcgatcgagc gcttcgtcgc gctccacctc ccgctcaagg aggcgcggcg
     7021 cgcctggacc gagagcttcg agctggtcta cgtgcgcgcc atgctcaagc tggt
//
LOCUS       DASVOR010001445         3920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_219424,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001445 DASVOR010000000
VERSION     DASVOR010001445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219424"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..474)
                     /locus_tag="VF516_27395"
     CDS             complement(<1..474)
                     /locus_tag="VF516_27395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8111498.1"
                     /translation="MPDIVLEARGLHKKFRKGELYTSLRDLVPALARRAVKGGLGKSE
                     FWALRDVNFQVERGEAFGIIGHNGAGKSTLLKHLCGILVPTSGELVVKGRLSALIEVG
                     AGFHPDLTGRDNIYLNGTILGMTRAEIRSKFDAIVEFSELADFLDTPVKRYSSGMY"
     gene            complement(474..1268)
                     /locus_tag="VF516_27400"
     CDS             complement(474..1268)
                     /locus_tag="VF516_27400"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8111499.1"
                     /translation="MLTMLRLLTRHRDLLYMLSRRDVTVKYKQSIMGFLWAILMPLLI
                     TSAGVVVKLAMTTVSGKGGMFREVLPVAIKAAPWAFFVASIRFSTQSLSNNHNLVTKI
                     AFPRAVFPLSAVASQLFDFLIAALVVVTVAVFGVSPTVYLLWVPVLLALLIAQVAGLA
                     LLLSAANLFFRDVKYLVEVVLTFAIFFTPVFYSVSDIVAKFGRTGELLLLNPVAPVLE
                     GLDAVIVGGHAPDPLWTGYAAIWALVLLIVTPAIFLRLEPRFAESV"
     gene            complement(1286..2068)
                     /locus_tag="VF516_27405"
     CDS             complement(1286..2068)
                     /locus_tag="VF516_27405"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX8111500.1"
                     /translation="MKVVLFCGGQGMRLRDYSDQLPKPLVPIGPRPILWHLMSYYARY
                     GHKDFVLCLGYRGTAIKDYFLKYDETVSNDFVMSGGGTQIELLRRDIADWRITFVDTG
                     QRASIGERLRAVRPHLDGEDAFLANYSDGLADLDLDLYLDYFRRRNKVATFLSVAAPH
                     TFHIVRADAEDHVEKLEAVGRSVVRINGGFFAFKREIFDHLREGEDLVLEPFDRLIAR
                     RELLAYPFDGFWRNMDTFKDRQDLEELWNQGSPPWTLRPPQR"
     gene            2215..3624
                     /locus_tag="VF516_27410"
     CDS             2215..3624
                     /locus_tag="VF516_27410"
                     /inference="COORDINATES: protein motif:HMM:NF024602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4038 domain-containing protein"
                     /protein_id="HEX8111501.1"
                     /translation="MSPSRCWVPGWFATAASLLAACSGASGPGGVTGDGGVTDGDGGT
                     LPGFDGPPPTGTFPLAVSADGGSLVTADGRPFLLHAEAAWSLIAQPDTAGAMQYLADR
                     RRRGVQAVLVNLIEHLYSDHHPANAAGDPPFTTAGDFSTPNEAYFAHADQVIDLAASQ
                     GMAVLLVPSYLGFNGGDEGWFHDMSLLTAAQCRSYGDFVGRRYATRHNIIWVWGGDYT
                     PPTDSPGETCMKSIRDGILAAEPGALATAHWQPESTSRDEPAFASSISLVGVYTYLLD
                     LQTCLTARAVTPRKPAFLIETCYENESIRSCPDTPADIRRRQWWAFLGCGAGEIVGNN
                     PIWKFGSGWPQQLASPASLGEQRLAAIAGMVAWQTLAPDSALVTAGQAPAGNDAELAI
                     TRTADHKQAVIYVPPAGASTITVDLGRMSGPVTATWQDPTADHSVGAGDGLTGSHAFT
                     TPGNNNGGDRDWVLVLTPP"
BASE COUNT          620 a         1401 c         1358 g          541 t
ORIGIN      
        1 gtacatcccc gacgagtagc gcttgaccgg cgtgtccagg aagtcggcga gctcggagaa
       61 ctcgacgatc gcgtcgaact tgctgcggat ctcggcgcgg gtcatgccga ggatcgtgcc
      121 gttgaggtag atgttgtcgc gcccggtgag gtcggggtgg aagccggcgc cgacctcgat
      181 cagcgccgac agccggccct tgacgaccag ctcgccggag gtcggcacca ggatgccgca
      241 caggtgcttg agcagcgtgc tcttgcccgc cccgttgtgg ccgatgatgc cgaacgcctc
      301 gccgcgctcg acctggaagt tgacgtcgcg cagcgcccag aactcgctct tgcccagccc
      361 gcccttgacc gcgcgccggg ccagcgccgg cacgaggtcg cgcagcgagg tgtagagctc
      421 gcccttgcgg aacttcttgt gcaggccgcg ggcctcgagg acgatgtccg gcatcacacg
      481 ctctcggcga acctcggctc gagccgcagg aagatggccg gggtgacgat caagagcacc
      541 agcgcccaga tcgccgcgta gccggtccac agcggatcgg gcgcgtggcc gccgacgatc
      601 accgcgtcga ggccctcgag cactggcgcg accgggttga gcagcagcag ctcgccggtg
      661 cggccgaact tcgcgacgat gtcggacacc gagtagaaca ccggcgtgaa gaagatcgcg
      721 aacgtcagca cgacctcgac caggtacttg acgtcgcgga agaacaggtt ggcggccgac
      781 agcaagagcg cgagcccggc gacctgcgcg atcaagagcg cgagcaggac cgggacccac
      841 aggaggtaca ccgtcggcga caccccgaac accgcgaccg tcacgacgac cagcgccgcg
      901 atcaggaagt cgaacagctg cgacgccacc gccgacagcg ggaacaccgc gcgcgggaac
      961 gcgatcttgg tgaccaggtt gtggttgttg gacaggctct gggtcgagaa ccggatcgac
     1021 gccacgaaga acgcccacgg cgcggccttg atcgcgaccg gcaggacctc gcggaacatc
     1081 ccgcccttgc ccgacaccgt cgtcatcgcg agcttgacca ccacgccggc cgaggtgatc
     1141 agcagcggca tcaggatcgc ccacaaaaag cccatgatcg actgcttgta cttcacggtc
     1201 acgtcgcgcc ggctcagcat gtagagcagg tcgcggtggc gggtgagcag gcggagcatc
     1261 gtcagcatgt ccggatcctg gcgggtcagc gctgcggcgg ccgcagggtc cacggcggcg
     1321 agccctggtt ccacagctcc tcgaggtcct ggcggtcctt gaacgtgtcc atgttgcgcc
     1381 agaacccgtc gaacgggtac gcgagcagct cgcgccgcgc gatcaggcga tcgaacggct
     1441 cgagcaccag gtcctcgccc tcgcgcaggt ggtcgaagat ctcgcgcttg aacgcgaaga
     1501 acccgccgtt gatccggacc accgagcggc cgaccgcctc gagcttctcg acgtggtcct
     1561 cggcgtcggc ccggacgatg tggaaggtgt gcggcgcggc gaccgacagg aaggtcgcga
     1621 ccttgttgcg gcgccggaag tagtcgaggt agaggtcgag gtcgaggtcg gccaggccgt
     1681 cgctgtagtt ggcgaggaac gcgtcctcgc cgtcgaggtg cgggcgcacc gcgcgcagcc
     1741 gctcgccgat gctcgcgcgc tggccggtgt cgacgaacgt gattcgccag tcggcgatgt
     1801 cgcggcgcag cagctcgatc tgcgtgccgc ccccggacat cacgaagtcg ttcgagaccg
     1861 tctcgtcgta cttgaggaag tagtccttga tcgccgtgcc gcggtagccc aggcacagga
     1921 cgaagtcctt gtggccgtag cgggcgtagt agctcatcag gtgccacagg atcggacgcg
     1981 ggccgatcgg caccagcggc ttgggcagct gatcggaata gtcgcggagc cgcatcccct
     2041 gcccgccgca aaacagcaca accttcatga gccgctactc cctcgagcgt tcatcgcgtc
     2101 actgtaaccg cagcggcaag cgcgcgaaac cgcgccggcc gggattcgcg tgcgccagcg
     2161 ctcgtgcacg accgtcgttg tctcggcccc gggccgcgac taggctatgc ggcgatgtca
     2221 ccatcgcgct gctgggtccc ggggtggttc gccacggccg cgagcctcct ggcggcctgc
     2281 tccggcgcgt cgggccccgg cggggtcacc ggcgatggcg gcgtgaccga cggggatggc
     2341 ggcacgctgc ccgggttcga tggcccgccg ccgaccggga cgttcccgct cgcggtgtcc
     2401 gctgatggtg gctcgctggt caccgccgac ggccggccgt tcctgctgca tgccgaggcg
     2461 gcgtggtcgc tgatcgcgca gcccgacacc gccggcgcga tgcagtacct cgccgaccgc
     2521 cgccgccgcg gcgtccaggc cgtgctggtc aacctgatcg agcacctcta ctcggaccat
     2581 cacccggcca acgccgccgg cgatccgccg ttcacgaccg ccggcgactt ctccacgccc
     2641 aacgaggcgt acttcgcgca cgcggaccag gtgatcgatc tcgcggcgag ccagggcatg
     2701 gccgtgctgc tggtcccgtc ctacctcgga ttcaacggcg gcgacgaggg ctggttccac
     2761 gacatgtcgt tgctgacggc cgcccagtgc cggagctacg gcgacttcgt cggccggcgc
     2821 tacgcgaccc ggcacaacat catctgggtc tggggcggcg actacacgcc gccgaccgac
     2881 tcgccgggcg agacctgcat gaagtcgatc cgcgacggca tcctcgcggc ggagccgggg
     2941 gcgctcgcga ccgcgcactg gcagcccgag tcgacctcgc gcgacgagcc cgcgttcgcc
     3001 agctcgatca gcctggtcgg ggtctacacg tacctgctcg acctgcagac gtgcctcacg
     3061 gcgcgggcgg tgacaccgcg caagccggcg ttcctgatcg agacctgcta cgagaacgag
     3121 tcgatccgga gctgtcccga cacgccggcc gacatccgcc gccgccagtg gtgggcgttc
     3181 ctcggctgcg gcgcgggcga gatcgtcggc aacaacccga tctggaagtt cggcagcggc
     3241 tggccgcagc agctcgcgtc gccggcgtcg ctcggcgagc agcggctcgc ggcgatcgcc
     3301 ggcatggtcg cctggcagac gctcgccccc gacagcgcgc tggtcaccgc cggccaggcc
     3361 ccggccggca acgacgccga gctcgcgatc acccgcaccg ccgatcacaa gcaggcggtg
     3421 atctacgtgc cgcctgcggg cgcctcgacg atcaccgtcg acctcggccg gatgagcggg
     3481 ccggtcaccg cgacctggca ggaccccacg gccgatcact cggtcggcgc cggcgacggg
     3541 ctcaccggca gccatgcgtt caccacgccg ggcaacaaca acggcggcga ccgcgactgg
     3601 gtgctggtgc tgaccccgcc gtagcgcgcg cgctcgctgc gatcggcgag atcgcacgcg
     3661 agccacggtg ggggctcgca accgtgcgca gctgtcacct cggacaatcg ccgatggcgg
     3721 catggcggat gcagtgacgt gctccaaagc ccatcatgcc gcccccggaa ccctctccgc
     3781 gacagctcgc gaagcgcgac ggccgcccac cgcgcctgtt cgcaacacgg aacgcgcggc
     3841 gcgcgcgccg gcgcaccctc gggcgctgcc ggcacctggt cgccggcgtg tcgctcgtgc
     3901 tcgcgacccc gacggccgcc
//
LOCUS       DASVOR010001446         2323 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_131478,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001446 DASVOR010000000
VERSION     DASVOR010001446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131478"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..1102
                     /locus_tag="VF516_27415"
     CDS             182..1102
                     /locus_tag="VF516_27415"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8111502.1"
                     /translation="MLVCLAIPVSLRSMAHAQPAAPASSEAAKHFQRGVTMYNEADYR
                     AALVEFRRAYETAPNAAVLYNIGQTYYQLQNYAAALVALGRYLTEAGPTAPHRREVEQ
                     TIDTLQTRVGKVAISTSTPGADVTVDDELVGKTPLEEPVLVSIGRRKITALRDGRVTE
                     TRFVDVAAGDTVTVSLAADARTPPPGTPEPGPRGPSPGMITTGWVVTGIAGAAAVGLG
                     GYAWLASRDLSSARNSNPVSSDAATAKQNDLASKSSKVSTFSHIADAAGAVAVIAGGV
                     TLYLSLSRSKEHETRVAITPNGIQIAGTFR"
     gene            1172..1462
                     /locus_tag="VF516_27420"
     CDS             1172..1462
                     /locus_tag="VF516_27420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX8111503.1"
                     /translation="MYLGIVKGTVVAERKAAGLEGKKLLLVQPVDEHGAAVGDVQAAV
                     DTVQAGDGDLVYLVGSREAALACDPWFVPVDAAIVGIVDGIGLPEAGPKARP"
     gene            1459..1731
                     /locus_tag="VF516_27425"
     CDS             1459..1731
                     /locus_tag="VF516_27425"
                     /inference="COORDINATES: protein motif:HMM:NF015289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HEX8111504.1"
                     /translation="MRYCRVVAPMWAAAKHPAFAGRPLFVVQPLDERGGDAGASFVAV
                     DHVQAGIGDRVVVLTDGNGVRQILKAGDIVPIRSIIVGIVDHVEPA"
     gene            1728..>2323
                     /locus_tag="VF516_27430"
     CDS             1728..>2323
                     /locus_tag="VF516_27430"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8111505.1"
                     /translation="MKPLRVQVAAIARQLHAGGWVANHDGNVTARDGARFLATPTATS
                     KRLIGDRDLIEVDARGQVHGPGKVFGEIGLHLAVYERRPDVGCVVHAHPPCATAISAS
                     PGNPIERPFLAEAVVSLGPRIPKLPYAQPGDAARAALAPWCELVDAVILGNHGAIAWG
                     ADPEQAFLRLELVEHLARIAAAAAPLGGVEPLPEAAIAP"
BASE COUNT          323 a          892 c          825 g          283 t
ORIGIN      
        1 cgcccgagcc gccgccgccg ccgccgcaga ctccgccgcc gcagcagccc gagctcgtca
       61 agccgcccag cgacatcagc ccgaccggcg gcagcaagcc gcgccgcccg atcgacccga
      121 gcaatccgta cgacaaatct ggaggaggac aatgagctgt cgacgtaccg tctgcctcgc
      181 ggtgctggtg tgcctcgcga tcccggtgtc gctgcgctcg atggcgcacg cccagcccgc
      241 ggcgccggcg agcagcgagg ccgccaagca cttccagcgc ggcgtgacca tgtacaacga
      301 ggccgactac cgcgcggcgc tggtcgagtt ccggcgcgcc tacgagaccg cccccaacgc
      361 cgcggtgctc tacaacatcg gccagaccta ctaccagctc cagaactacg cggccgcgct
      421 ggtcgcactc ggccgctacc tgaccgaggc cggacccacc gcgccgcacc gccgcgaggt
      481 cgagcagacg atcgacacgc tgcagacccg ggtcggcaag gtcgcgatct cgaccagcac
      541 gcccggcgcc gacgtcacgg tcgatgacga gctggtcggc aagacgccgc tcgaggagcc
      601 ggtgctggtc agcatcggcc gccgcaagat caccgcgctg cgcgacggcc gggtcaccga
      661 gacccggttc gtcgacgtcg cggcggggga cacggtcacc gtgtcgctgg ccgccgacgc
      721 gcgcaccccc ccgcccggga cgcccgagcc cggcccccgc ggcccgagcc cgggcatgat
      781 caccaccggc tgggtcgtga ccggcatcgc cggcgcggcc gcggtcggcc tcggcgggta
      841 cgcatggctg gcgtcgcgcg acctgtcgag cgcgcgcaac agcaacccgg tgtcgtccga
      901 tgcggccacc gccaagcaga atgatctcgc cagcaagagc agcaaggtct cgaccttctc
      961 gcacatcgcc gacgccgccg gcgccgtggc ggtgatcgcg ggcggcgtca cgctctacct
     1021 gtcgctgtcg cgctcgaagg agcacgagac ccgcgtcgcg atcacgccga acgggatcca
     1081 gatcgccggc acgttccgct agccgcgggc tcaccgcaag gcgggacagg cgggtttgac
     1141 ggcgcaccgt ccgcgcttta cgctcgccgc gatgtatctg ggcatcgtca agggcaccgt
     1201 ggtcgccgag cgcaaggcgg ccggcctgga gggcaagaag ctgctgctcg tccagccggt
     1261 cgacgagcac ggcgccgcgg tcggcgacgt ccaggccgcg gtcgacaccg tgcaggccgg
     1321 ggacggcgac ctggtctacc tggtcggctc gcgcgaggcg gcgctggcct gcgatccgtg
     1381 gttcgtgccg gtcgacgccg cgatcgtcgg gatcgtcgac gggatcggcc tgcccgaggc
     1441 cgggcccaag gccaggccat gaggtactgc cgggtggtcg cgccgatgtg ggcggcggcc
     1501 aagcaccccg cgttcgccgg ccggccgctg ttcgtggtcc agccgctcga cgagcgcggc
     1561 ggcgacgccg gcgcgagctt cgtcgcggtc gaccacgtcc aggccgggat cggcgatcgc
     1621 gtcgtcgtgc tcaccgatgg caacggcgtg cgccagatcc tcaaggccgg cgacatcgtg
     1681 ccgatccggt cgatcatcgt cggcatcgtc gatcacgtgg agccggcgtg aagccgctgc
     1741 gggtccaggt cgcggcgatc gcgcgccagc tccacgccgg cggctgggtc gccaaccacg
     1801 acggcaacgt caccgcgcgc gacggggcgc gcttcctggc gacgccgacc gcgacgtcca
     1861 agcggttgat cggtgaccgc gatctgatcg aggtcgacgc ccgcggccag gtccacggcc
     1921 ccggcaaggt gttcggcgag atcggcctgc acctcgcggt ctacgagcgc cggcccgacg
     1981 tcggctgcgt ggtccacgcc cacccgccgt gcgccaccgc gatcagcgcg agccccggca
     2041 acccgatcga gcggccgttc ctcgccgagg ccgtggtgtc gctcggcccg cgcatcccca
     2101 agctgcccta cgcccagccc ggcgatgccg cgcgcgccgc gctggcgccc tggtgcgagc
     2161 tggtcgacgc cgtgatcctc ggcaaccacg gcgcgatcgc gtggggcgcc gaccccgagc
     2221 aggcgttcct ccggctcgag ctggtcgagc acctggcgcg gatcgcggcc gccgccgcgc
     2281 cgctcggtgg ggtcgagccg ctgcccgagg ccgcgatcgc gcc
//
LOCUS       DASVOR010001447         4605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_482531,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001447 DASVOR010000000
VERSION     DASVOR010001447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4605
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482531"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VF516_27435"
     CDS             complement(<1..801)
                     /locus_tag="VF516_27435"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HEX8111506.1"
                     /translation="MFARLGFGLGLRLPHYHDVVEGDPRVDWWEVISENFLVAGGNPR
                     RVLRLVRERWPVVLHGVSLSIGSVDPVDADYLARLAALADEVDPPYVSDHLCWSSLGG
                     HSAHDLWPMPYTEEALDLVVDKVGRVQDRLRRRILLENPSSYVTFAASSLGEAEFLAE
                     VARRADCGILLDVNNVYVSSTNHGWDAHAYLAAIPPERVAQIHLAGHSDHGTHLLDTH
                     DHPVCEAVWRLYGEAIARFGAVSTMIERDDDIPELAELVGELDRARQIA"
     gene            complement(806..1291)
                     /locus_tag="VF516_27440"
     CDS             complement(806..1291)
                     /locus_tag="VF516_27440"
                     /inference="COORDINATES: protein motif:HMM:NF016089.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8111507.1"
                     /translation="MIAHQETNTMLTSIKTLYQRALRSCAKLDWLGPLVVRLIIGVAF
                     FFDGKGKLENLDKVTSFFADDLHIPFPHANAVFVSVVELVGGLLLVIGLGTRIAALLL
                     AATMAVAFVTAIVPHSDSVLDLFSTIELTYLAIFVWLIVSGGGPISVDHLIARRESQV
                     Q"
     gene            1681..1974
                     /locus_tag="VF516_27445"
     CDS             1681..1974
                     /locus_tag="VF516_27445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111508.1"
                     /translation="MNGKAIAMTVAGLLLAVGPRVANADDKTKEAKVKCGGINSCKGK
                     GACAGASNSCSGQNGCKGKGWVETSAKECKDKKGTVVADKDAKGKDTKAPDKK"
     gene            2267..2458
                     /locus_tag="VF516_27450"
     CDS             2267..2458
                     /locus_tag="VF516_27450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111509.1"
                     /translation="MSGEGEKMVMLVADDGSFARALPAEAIVPSRVVVTAEGAVHLAE
                     RMTAPRNPTRAMLALFVGK"
     gene            complement(2489..3325)
                     /locus_tag="VF516_27455"
     CDS             complement(2489..3325)
                     /locus_tag="VF516_27455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8111510.1"
                     /translation="MTATPDGGELLARARTGDRTAFDALVAPHLPRLRSFLHRLVAHP
                     DDAEDLAQDAVLQAFRKLAGFRGESAFSTWLFAIAHRLALTHLRTRKQRWPATIQLEM
                     HDRAASSAAFGAELAGEMSHPAFRYEVAEHIAYCFACIARSLDHEDSAALVLREVLDV
                     PHCEAARIVDLSESVFRHRLAAARRTMTEAFDGLCALINKQGACHQCSTLRGLCPPDR
                     QGPPAEPLGDVTTDDPDALLDRRLAAVRRADVVAGSTARMHTVMLRFIARHADPPTSS
                     DS"
     gene            complement(3322..3852)
                     /locus_tag="VF516_27460"
     CDS             complement(3322..3852)
                     /locus_tag="VF516_27460"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8111511.1"
                     /translation="MPTDAPPAGPGRLERSEMGGGDPDGSAGGAGGKAPRGIDRAWAI
                     VFARLVLALIFFMAGVYKVFEQGPLEHARKWFLPFRDTFLPVWSLWAVGTAIPFIELV
                     AGGLLLIGWRIREALLALGAVLVIVTFGHLLHEPLYPFHEHVIPRLALLVFVLAMPRA
                     DDRFSLDRLLQRRARS"
     gene            complement(3900..>4605)
                     /locus_tag="VF516_27465"
     CDS             complement(3900..>4605)
                     /locus_tag="VF516_27465"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8111512.1"
                     /translation="IAPVLSGLAGATVVHSIPAVSALPPGQAMRAALQAAYGAGAGCF
                     VLFSSSGLYGSQPDDEVWIDEDTPVATDDPPMNNVKNDELEVERCGFDRLRTVILRLA
                     PVYGPGRGLRERIRKGEFRLLDDGQHATSRIHIDDVARVVFAAEDRAPRGARYLVADD
                     EPTTQGEYTRWLCERLGLPVPPSRQIFEPGKPRVAHRNRRIRNARLKQDLGIELRYPT
                     FREGEAAIEAELAAQG"
BASE COUNT          734 a         1543 c         1725 g          603 t
ORIGIN      
        1 tgcaatctgg cgcgcccggt cgagctcgcc gaccagctcg gcgagctccg ggatgtcgtc
       61 gtcgcgctcg atcatcgtcg acaccgcgcc gaaccgcgcg atcgcctcgc cgtagagccg
      121 ccacaccgcc tcgcacaccg gatggtcgtg ggtgtcgagc aggtgggtgc cgtgatcgct
      181 gtggccggcg aggtggatct gcgcgacgcg ctccggcggg atcgccgcca ggtaggcgtg
      241 cgcgtcccag ccgtggttgg tcgagctgac gtagacgttg ttgacgtcga gcaggatgcc
      301 gcagtcggcg cgccgcgcga cctcggcgag gaactcggcc tcgccgagcg agctcgcggc
      361 gaacgtgacg tagctcgacg ggttctcgag caggatccgc cggcgcaggc ggtcctggac
      421 ccggccgacc ttgtcgacca cgaggtcgag cgcctcctcg gtgtacggca tcggccacag
      481 atcgtgggcc gagtggccgc cgagcgacga ccagcacagg tgatccgaca cgtagggcgg
      541 gtcgacctcg tcggcgagcg ccgccagccg cgcgaggtag tcggcgtcga ccggatcgac
      601 cgagccgatc gacagcgaca cgccgtgcag cacgaccggc cagcgctcgc ggaccaggcg
      661 cagcacgcgc cgcgggttgc cgccggcgac caggaagttc tcgctgatca cctcccacca
      721 gtcgacgcgc ggatcgccct cgaccacgtc gtggtagtgc ggcaggcgaa gcccgaggcc
      781 gaagccgagg cgagcgaaca tggcgctact gtacctgact ctcgcggcgg gcgatcaggt
      841 ggtcgaccga gatcgggccg ccgccgctga cgatcagcca gacgaagatc gccaggtagg
      901 tcagctcgat cgtgctgaac agatcgagga cgctgtcgct gtggggaacg atcgcggtga
      961 cgaacgcgac cgccatcgtg gcggccagca gcagcgccgc gatccgcgtg cccaggccga
     1021 tcaccagcag caggccgccg acgagctcga ccaccgacac gaacaccgcg ttggcgtgcg
     1081 ggaacgggat gtggagatca tcggcgaaga agctggtgac cttgtcgagg ttctccagct
     1141 tgcccttgcc atcgaagaag aacgccacgc cgatgatgag tcggaccacc agcgggccga
     1201 gccagtcgag cttggcgcac gaccggagcg cgcgctgata cagggtcttg atcgaggtga
     1261 gcatcgtgtt cgtctcctga tgggcgatca tggtcgggct tgctacgccg cgcgatcgga
     1321 gcagtaacgc ccttcgcgac ggcgatgcgc gcggacggga cgacgcagcg cggcatcgcg
     1381 acgggggcgc acggaacgcg atccattccg cgcgcccgcg cgtaacaccc gccggggacg
     1441 gacgtaggca gggccaaccc ccgtggtttc caccgatgcg atcgagacga ccgggagctt
     1501 caggctgcga gcccgatgcg gcgccggatg gcgcttcacc acgccatgta ggtgcatggc
     1561 gcactcaaag ctcgttgaac aagcgttctc ggcgatcgtc gcattccctg cgcaacggat
     1621 ctcgtgtact cgaaaccaac gggagaccac gaggtccacg aaggagcaat gaagatgacg
     1681 atgaacggaa aagcgatcgc gatgaccgtg gcgggacttc tgctggccgt ggggccgcgc
     1741 gtggccaacg ccgacgacaa gaccaaggaa gccaaggtca agtgcggtgg catcaactcg
     1801 tgcaagggca agggcgcgtg cgccggcgcc tcgaacagct gctcgggtca gaacggctgc
     1861 aagggcaagg gctgggtcga gacctcggcc aaggagtgca aggacaagaa gggcaccgtg
     1921 gtcgccgaca aggacgccaa gggcaaggac accaaggcgc ccgacaagaa gtgacgggcc
     1981 acggcgcgcg atcgggctga tcggtcgcgc tgccgcatga cgctcgtcgg cggtcgcgcc
     2041 gaccgcaggc atctgcagtc gatcgcgccg cgcaatcacc ggcgcccgca tccgctcggg
     2101 cacccggctc gcgggcacgg ccgcgccgaa ccgcgcgccg cgagatcgcg agcccccacc
     2161 gctgcgtgcc tggttcgacc ggatcaccgc gcgcccgtcc tggcaggcga cccagccctc
     2221 gcggtgacgg ctcgaaggat caccgccttg cgctatgatc catgtcatgt ccggcgaggg
     2281 cgagaagatg gtcatgctgg ttgccgacga cggatccttt gcgcgggccc ttccagcgga
     2341 ggcgatcgtt ccgtcgcggg tcgtcgtcac agccgagggc gcggtacacc tcgccgagcg
     2401 catgacagcg ccacgtaatc cgacgcgtgc catgctcgca ttgtttgtcg ggaagtgagt
     2461 ccgctcgcgt cgaagcgtcg ctgcgcgatc acgaatccga gctggtcggc gggtcggcgt
     2521 ggcgggcgat gaagcgcagc atcacggtgt gcatgcgcgc ggtgctgccg gccacgacgt
     2581 ccgcgcggcg gacggcggcg agccggcggt cgagcagcgc gtcggggtca tcggtggtga
     2641 cgtcgccgag cggctcggcc ggcgggccct ggcggtcggg cgggcacagc ccgcgcagcg
     2701 tgctgcactg gtggcaggcg ccctgcttgt tgatcagcgc gcacaggccg tcgaacgcct
     2761 cggtcatcgt gcgccgcgcc gcggccagcc ggtggcggaa caccgactcg gacagatcga
     2821 cgatccgggc ggcctcgcag tgggggacgt cgaggacctc gcgcaggacg agcgcggcgc
     2881 tgtcctcgtg gtcgagcgag cgcgcgatgc acgcgaagca gtaggcgatg tgctcggcga
     2941 cctcgtagcg gaacgcaggg tgcgacatct cgccggcgag ctcggcgccg aacgctgcgc
     3001 tggacgccgc gcggtcgtgc atctcgagct ggatcgtcgc gggccagcgc tgcttgcggg
     3061 tgcggaggtg ggtcagtgcg aggcggtgcg cgatcgcgaa cagccaggtg gagaacgccg
     3121 actcgccgcg gaagccggcg agcttgcgga acgcctggag cacggcgtcc tgcgcgaggt
     3181 cctcggcgtc gtcggggtgg gcgaccaggc ggtgcaggaa gctgcgcagc cggggcaggt
     3241 ggggcgcgac cagtgcgtcg aacgccgtcc ggtcgccggt ccgggcgcgc gcgaggagct
     3301 cgccgccgtc gggggtggcg gtcatgaccg cgcccggcgc tgcagcaggc ggtcgagcga
     3361 gaaccggtcg tcggcgcgcg gcatggcgag cacgaacacc agcagcgcga gccgcgggat
     3421 gacgtgctcg tggaacggat acagcggctc gtggagcagg tggccgaagg tgacgatcac
     3481 gagcacggcg ccgagcgcga gcagggcctc gcggatgcgc cagccgatga ggagcaggcc
     3541 gccggcgacg agctcgatga acgggatcgc ggtcccgacc gcccacagcg accacaccgg
     3601 caggaaggtg tcgcggaacg gcaggaacca cttgcgggcg tgctcgagcg gtccctgctc
     3661 gaagaccttg tagacgccgg ccatgaagaa gatcagcgcg agcaccaggc gcgcgaacac
     3721 gatcgcccag gcgcgatcga tcccacgggg ggccttgccc cccgccccgc cggcggagcc
     3781 gtcggggtcc ccacccccca tctcgcttcg ctcgagacga ccgggtccgg cgggcggcgc
     3841 atcggtgggc atggcccgat ggtactcctc accttcccgg tagggacagg gttgcgcggt
     3901 cagccctgcg cggcgagctc ggcctcgatc gcggcctcgc cctcgcggaa cgtcggatag
     3961 cgcagctcga tcccgagatc ctgcttgagc cgggcgttgc ggatccggcg gttgcggtgg
     4021 gcgacgcgcg gcttgccagg ctcgaagatc tggcgcgacg gcggcaccgg gaggccgagg
     4081 cgctcgcaca gccagcgggt gtactcgccc tgggtcgtcg gctcgtcgtc ggcgaccagg
     4141 tagcgcgcgc cgcgcggggc gcggtcctcg gcggcgaaca cgacgcgcgc gacgtcgtcg
     4201 atgtggatcc gcgacgtcgc gtgctggccg tcgtcgagca ggcggaactc gcccttgcgg
     4261 atccgctcgc gcaggccgcg gccggggccg tagaccgggg cgagccgcag gatcacggtg
     4321 cgcagccggt cgaagccgca gcgctcgacc tcgagctcgt cgttcttgac gttgttcatc
     4381 ggcggatcgt cggtcgcgac cggagtgtcc tcgtcgatcc agacctcgtc gtcgggctgg
     4441 ctgccgtaca gccccgacga gctgaacagg acgaagcagc cggcgccggc gccgtacgcg
     4501 gcctgcagcg cggcgcgcat cgcttgcccg ggcggcagcg cgctcaccgc cgggatcgag
     4561 tgcacgaccg tcgcgccggc gagcccgctg agcacgggcg cgatc
//
LOCUS       DASVOR010001448         5270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_15600,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001448 DASVOR010000000
VERSION     DASVOR010001448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5270
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15600"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VF516_27470"
     CDS             complement(<1..423)
                     /locus_tag="VF516_27470"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8111513.1"
                     /translation="MLRHLRVTNFAILSDVAIELGEGMNVLTGETGAGKSLIVEAVNL
                     LRGGRASADIPRAGTDEAVVEAIVAIPEDLAARVAGVLEGAGASGGDPEVVIRRVIQR
                     GGRSRTYVNGALTTLARLAELGALLIDLSGQHQHQGLVD"
     gene            complement(417..1283)
                     /locus_tag="VF516_27475"
     CDS             complement(417..1283)
                     /locus_tag="VF516_27475"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HEX8111514.1"
                     /translation="MKPDKSEAGVLLEELVPWLRDQGHLPVVTSEDQISPTGCEVVPE
                     EELGAAVDLAVVLGGDGTMLRAARLVADHGRPVLGINLGQLGFLVGFAPSHAKQALAE
                     ALAGRLPQTRRMRLAVTFRRRGAEPVVRYALNDAVLHQGAMARLVKIDAFVDGEFVAS
                     YRADGLIVATPTGSTAYNMAAGGPIVLPGQEAMTLTPICAHALTHRPLVISASSTIRL
                     QLGVDVRGALLTVDAQWSHSFLHGDIAEIAAASAPLIMFTSKQSFFDVMRDKLHWGAR
                     SDRRARKDGDGC"
     gene            1433..1708
                     /locus_tag="VF516_27480"
     CDS             1433..1708
                     /locus_tag="VF516_27480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111515.1"
                     /translation="MSAASVVQFIDETTAGEMLREHEVRLHASATVKARELLQLRFGD
                     NADRACEAFNQGAFRMQIAGSPIDSLDAHVHLVPGLAVKLIRLTAEQ"
     gene            1844..2296
                     /gene="rplM"
                     /locus_tag="VF516_27485"
     CDS             1844..2296
                     /gene="rplM"
                     /locus_tag="VF516_27485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEX8111516.1"
                     /translation="MMTTQKSSWLTKEAGEAQREWYVIDATGHTLGRLASRIALVLRG
                     KHKATYTPNVDMGDFVIVLNAGGVKLTGTKPDKKLYQHHTLFPGGIKTRLAKDVLADD
                     PERAIREAVWGMMPKGPLGRRILKKLKIYRGTEHDHAAQKPRVLEIKN"
     gene            2321..2713
                     /gene="rpsI"
                     /locus_tag="VF516_27490"
     CDS             2321..2713
                     /gene="rpsI"
                     /locus_tag="VF516_27490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEX8111517.1"
                     /translation="MQTTKTYATGRRKTAVARVWLTAGDGKFTINERSDEDYFSRPTA
                     RMVIRQALEEVALLAQYDVHATVRGGGIASQAGAIRHGITRALMKVDSELRPKLKAAG
                     YVTRDPRKKERKKYGQKAARARFQFSKR"
     gene            2817..4244
                     /locus_tag="VF516_27495"
     CDS             2817..4244
                     /locus_tag="VF516_27495"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8111518.1"
                     /translation="MRFRATILVKLLVALVVPTVALFTLFAFVAFEVSRRDLDHELGQ
                     RLEAIAASATTQIRGKYLTELVPGNEEDRGYQNVIKKLEAIADATGAQLFVFDDQYNS
                     RGDTAPGVAIGTHYFRAELDRAELVRVFELGRTASSVTFIGNDDKIYKTGYAPVRASE
                     TEPQIVLALGAQAPASYFARLADLRDRLFAWGTGLLAVSVMAAVVATLLITRNVRRLV
                     AAAERIGSGNLRDPVRVSSDDEIGVLGETMERMRQQLAERDARTQQMLAGIAHEVRNP
                     LAGMTLFAGILRDELPEDDARRGHVERIRRELGYLERVVNDFLEYARRPKPELRDEPC
                     DALLAEVAQLTSTEDIAVEAVACDGLVARADRGQIRRALLNLAKNAVQAATAAGHRGR
                     GAVRLSAEERGDELALVVWNRGHEIPPEICDRLFEPFYTTREKGTGLGLAFVREIAAD
                     HGGRIEVKSASGETTFTLALPLAEV"
     gene            4420..>5270
                     /locus_tag="VF516_27500"
     CDS             4420..>5270
                     /locus_tag="VF516_27500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8111519.1"
                     /translation="MPTILIIDDNETVRDGLAHTVKKLGHEAVTATGGVAGIEAFKAR
                     RPDFVISDLKMDGPSGVDVLRAMTAIDPDVPIMIITGFGSVETAVEAMKLGAFDFITK
                     PIQPEVVRLKTERALELCAARRGRRKADALTDYLTAEADAHFPELVGNGEKMQAVRRT
                     VEKIAASDTTVFICGESGTGKELVARAIHRLSKRASGPFIKVNCGALTETLLESELFG
                     HEKGAFTGAVKQKPGRFELADNGTLFLDEVGDVPPAMQVKLLRALQEQEFERVGGEAP
                     IKVDVRVV"
BASE COUNT          849 a         1907 c         1791 g          723 t
ORIGIN      
        1 atcgaccagg ccctggtgct ggtgctggcc cgacagatcg atcagcagcg cgccgagctc
       61 ggcgagccgc gccagcgtgg tcagcgcccc gttgacgtag gtccggctcc ggccgccgcg
      121 ctggatcacc cggcggatca ccacctcggg atcgcccccg ctggccccgg cgccctcgag
      181 caccccggcg acgcgcgccg caaggtcctc ggggatcgcg acgatcgcct cgacgaccgc
      241 ctcgtcggtc cctgcccgcg gaatgtcggc cgacgctcgc ccgccgcgca gcaagttgac
      301 cgcctcgacg atcagcgact tgcccgcgcc ggtctcgccg gtgagcacgt tcatgccctc
      361 gccgagctcg atcgccacgt cgctgaggat cgcgaagttg gtgacccgca ggtgcctcag
      421 catccgtcgc cgtctttccg ggcgcgccgg tcggagcgcg cgccccagtg cagcttgtcg
      481 cgcatgacgt cgaagaagct ctgcttcgag gtgaacatga tcagcggcgc gctcgccgcg
      541 gcgatctccg cgatgtcgcc gtggaggaac gaatggctcc actgcgcgtc gaccgtgagc
      601 agcgcgccgc gcacgtcgac cccgagctgg agccggatcg tgctgctcgc cgagatcacg
      661 agcggccggt gggtcaacgc gtgcgcgcag atcggcgtca gcgtcatcgc ctcctggccg
      721 ggcagcacga tcggcccgcc ggccgccatg ttgtaggcgg tcgagccggt cggcgtcgcg
      781 acgatcaacc cgtcggcgcg gtacgatgcg acgaactcgc cgtcgacgaa cgcatcgatc
      841 ttcacgagcc gcgccatcgc gccctggtgc agcaccgcgt cgttgagcgc gtagcggacc
      901 accggctcgg cgccccgccg ccggaacgtc accgccagcc gcatccgccg ggtctgcggc
      961 agccggcccg cgagcgcctc ggcgagcgcc tgcttggcgt ggctcggtgc gaacccgacc
     1021 aggaagccga gctggccgag gttgatcccg agcaccgggc ggccgtggtc ggcgaccagc
     1081 cgcgccgcgc gcagcatggt cccgtcgccg cccagcacga ccgcgaggtc gaccgccgcg
     1141 ccgagctcct cctccggcac cacctcgcac ccggtgggcg agatctggtc ctcggaggtc
     1201 accaccggga ggtgcccctg atcgcgcaac cacggcacca gctcctccag caggacccct
     1261 gcttcggatt tgtcgggctt caagatgaaa ccaaccctgt acacgcccct gtcctaccac
     1321 acccccctga cactgaacta ccgtgcaggt caacccggct gggcgagccc tgcgtgaaac
     1381 tggcagccgg ccgatacggc gccggccgct tccccatata aggtcccgcg ccatgtccgc
     1441 agcctccgtc gtgcaattca tcgatgaaac caccgccggc gagatgcttc gcgagcacga
     1501 ggtacggctc catgcctcgg cgaccgtcaa ggcccgggag ctgctccagc tccggttcgg
     1561 cgacaacgcc gaccgcgcct gcgaggcgtt caaccagggt gccttccgga tgcagatcgc
     1621 cggctcgccg atcgactcgc tcgacgccca cgtccacctc gtccccggcc tggcggtcaa
     1681 gctgatccgg ctcaccgccg agcaatgatt ccgggtgacg gcccgtccac cgggcgcccg
     1741 cccgtccccg gcccctgccc gtgccggggc gcaccgccgc ggctgcgtgc acttgacagg
     1801 cgtttcgcgg tcttgtaaac gatcgcgctt cgagcaaacg cccatgatga cgacccagaa
     1861 gagctcctgg ctcaccaaag aggccggcga ggcgcaacgc gagtggtacg tgatcgatgc
     1921 gaccggccat acgctgggtc gcctcgcctc gcggatcgcg ctggtgctcc gcggcaagca
     1981 caaggccacc tacaccccga acgtcgacat gggcgacttc gtgatcgtgc tcaacgccgg
     2041 cggggtcaag ctcaccggca ccaagccgga caagaagctg taccagcacc acaccctgtt
     2101 ccccggcggc atcaagaccc ggctggcgaa ggacgtgctc gccgacgatc ccgagcgtgc
     2161 gatccgcgag gcggtgtggg gcatgatgcc caagggcccg ctcggccgcc gcatcctcaa
     2221 gaagctcaag atctatcgcg gcaccgagca cgatcatgcg gcgcagaagc cgcgcgtcct
     2281 cgagatcaag aactaggcgc aagtcgccca gaggcaaacg atgcagacga cgaagacgta
     2341 tgccaccggc cgccgcaaga ccgccgtggc acgtgtgtgg ttgaccgccg gcgacggcaa
     2401 gttcacgatc aacgagcgca gcgacgagga ctacttctcg cggccgaccg cgcgcatggt
     2461 gatccgccag gcgctggagg aagtcgcgct gctcgcccag tacgatgtcc atgccaccgt
     2521 ccgcggcggc ggcatcgcct cgcaggccgg cgccattcgc cacggcatca cccgcgcgct
     2581 gatgaaggtc gacagcgagc tgcgacccaa gctcaaggcg gccggctacg tcacccgcga
     2641 cccgcgcaag aaggagcgca agaagtacgg ccagaaggcc gcgcgcgctc gcttccagtt
     2701 cagcaagcgc tgatcgcagc cgcacgcagc cacgcggttc gttgcgcgaa gggctgccag
     2761 gagcaggcag ggcacagcgc gcggagccat cggcctggtg tagtaggctt tccgccgtgc
     2821 ggtttcgcgc gacgatcctc gtcaagctcc tggtcgcgct ggtcgtgccg acggtggcgc
     2881 tgttcaccct gttcgcgttc gtcgcgttcg aggtcagccg gcgcgacctc gaccacgagc
     2941 tcggccagcg cctggaggcg atcgccgcgt cggcgaccac ccagatccgc ggcaagtacc
     3001 tgaccgagct ggtgcccggc aacgaggagg accgcggcta ccagaacgtg atcaagaagc
     3061 tcgaggcgat cgcggacgcg accggcgcgc agctgttcgt gttcgacgac cagtacaact
     3121 cgcgcggcga caccgcgccc ggcgtggcga tcggcaccca ttacttccgc gccgagctcg
     3181 atcgcgccga gctggtccgg gtgttcgagc tcggccggac ggcatcgtcg gtgacgttca
     3241 tcggcaacga cgacaagatc tacaagaccg gctacgcgcc ggtgcgcgcg tcggagaccg
     3301 agccgcagat cgtgctcgcg ctcggcgccc aggcgccggc gtcgtacttc gcgcggctcg
     3361 ccgacctgcg cgaccggctg ttcgcgtggg gcaccggcct gctcgcggtc agcgtgatgg
     3421 cggccgtggt cgcgacgctg ctgatcaccc gcaacgtgcg ccggctggtc gccgcggccg
     3481 agcggatcgg cagcggcaac ctgcgcgatc cggtgcgggt gtcgtccgac gacgagatcg
     3541 gcgtgctcgg cgagaccatg gagcggatgc gccagcagct cgccgagcgc gacgcgcgga
     3601 cccagcagat gctcgcgggc atcgcgcacg aggtccgcaa cccgctcgcc ggcatgacgc
     3661 tgttcgccgg gatcctgcgc gacgagctgc cggaggacga cgcgcggcgc ggccacgtcg
     3721 agcggatccg gcgcgagctc ggctacctcg agcgcgtggt caacgacttc ctggagtacg
     3781 cccggcggcc caagccggag ctgcgcgacg agccgtgcga cgcgctgctc gccgaggtcg
     3841 cccagctgac cagcaccgag gacatcgcgg tcgaggccgt ggcgtgcgac ggcctggtcg
     3901 cgcgggccga tcgcggccag atccgccgcg cgctgctcaa cctggccaag aacgcggtcc
     3961 aggccgccac cgccgccggt caccgcggcc gcggcgcggt gcggctgtcc gccgaggagc
     4021 gcggcgacga gctggccctg gtggtctgga accgcggtca cgagatcccg ccggagatct
     4081 gcgaccggct gttcgaaccg ttctacacga cccgcgagaa aggcaccggg ctgggcctgg
     4141 cgttcgtgcg agagatcgcc gccgatcacg gcggccggat cgaggtcaag agcgcttccg
     4201 gagagaccac gttcacgctc gcgctccctc tcgccgaggt gtgacgcgca atcattgcat
     4261 ctccgaagct ggctcggtcg caatgactta catctctcac cgcgtcgctg tatgccggct
     4321 gacgtggtga acctgcgtag cgaggggtcg aacctctcgg ccctctggca ggttgcggca
     4381 cttcgctaca gcggccggtg tacgatggaa cgtgcgagca tgcccacgat cctgatcatc
     4441 gacgataacg agaccgtgcg cgacggcctc gctcacaccg tcaagaaact ggggcacgag
     4501 gcggtcaccg ccacgggagg cgtcgccggg atcgaggcat tcaaggccag gcgccctgac
     4561 ttcgtcatct ccgacctcaa gatggacggg ccatccggag tcgacgtgct acgggcgatg
     4621 acggcgatcg accccgacgt gccgatcatg atcatcaccg ggttcggctc ggtcgagacc
     4681 gcggtcgagg ccatgaagct cggcgcgttc gacttcatca ccaagccgat ccagcccgag
     4741 gtggtccggc tcaagaccga gcgcgcgctc gagctgtgcg ccgcccggcg cggccgccgc
     4801 aaggccgacg cgctgaccga ctacctgacc gccgaggccg acgcccactt ccccgagctg
     4861 gtcggcaacg gcgagaagat gcaggcggtg cgccgcacgg tcgagaagat cgcggcgagc
     4921 gacaccacgg tgttcatctg cggcgagagc ggcaccggca aggagctggt cgcgcgcgcg
     4981 atccaccggc tcagcaagcg cgcctccggg ccgttcatca aggtcaactg cggcgcgctg
     5041 accgagaccc tgctggagag cgagctgttc ggccacgaga agggcgcgtt caccggcgcg
     5101 gtcaagcaga agcccggccg gttcgagctc gccgacaacg gcacgctgtt cctcgacgag
     5161 gtcggcgacg tgccgcccgc gatgcaggtc aagctgctgc gcgcgctgca ggagcaggag
     5221 ttcgagcggg tcggcggcga ggcgccgatc aaggtcgacg tccgcgtcgt
//
LOCUS       DASVOR010001449         2899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_335935,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001449 DASVOR010000000
VERSION     DASVOR010001449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335935"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VF516_27505"
     CDS             complement(<1..369)
                     /locus_tag="VF516_27505"
                     /inference="COORDINATES: protein motif:HMM:NF015210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEX8111520.1"
                     /translation="MASDLTRQLKSQRRTVDAEHFDLTLRELLRMFEDGALVRAPQHQ
                     RELHWDTGSQSRFIESLFLGFAVPSIFVAANTDGTWEVIDGVQRLSTLAHFGTTRPGV
                     LRELGRHEPLTLTGLDRLTEF"
     gene            complement(387..2411)
                     /locus_tag="VF516_27510"
     CDS             complement(387..2411)
                     /locus_tag="VF516_27510"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8111521.1"
                     /translation="MTASEPLILGMSASHNGAVCVLRGREIVVAIQEERLTREKRARV
                     YGSRSSLAVPYCLRAAGIAPEALAGTAISVQGYASAPEQQLVANPDLAGYAGPPPLRV
                     AHHRAHAVSAFSMSGFADAAVLVVDGLGSPWRDLDAGERAVCRKADPSGWETISIYGA
                     DGDAIVPLEKHLAPDRRWLQRRPKRMPRFGSLGGMYSAVAVQLFGDALEAGKVMGLAP
                     YGRATAPVDTFLRFDGDDLVFSDATADRFVLGKRWPERERAYRDLAASVQSALEHALL
                     GLARRARALTGSRNLCFAGGVALNSVANERLIREVGFEHVYIPAAAEDSGVAIGAAYE
                     CLRAVTSTYRARRVRHDAVGAPYTAADVSAAVRATPAIREVVAGPDAVGAAAKRLARG
                     DLGGWFVGRSELGPRSLGQRSILADPRLANGKEVLNRRVKHREAFRPFAPAILEEHAA
                     DWFELGPTAESPFMLRVVPFHEHVRARVPAVVHVDGTGRLQTVARDVPELHALISAFH
                     AQTGVPMLLNTSFNVMGEPIVETPEDALWCFLATGLDFVVVEHRLFVKKPGYESPLDL
                     VPVLAARRAIVELPVTGAKLQLDAPDGEIGFVVVTPWGETTVRVPAEQLAVISQIDGK
                     RTGHDIQRSLARSGVTPERVLAVLCALRRSRVIAFRGFRPRRGGDKRGRR"
     gene            complement(2470..>2899)
                     /locus_tag="VF516_27515"
     CDS             complement(2470..>2899)
                     /locus_tag="VF516_27515"
                     /inference="COORDINATES: protein motif:HMM:NF026213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HEX8111522.1"
                     /translation="TLMNRGARLDEVIASVAPPAALAARPYLSPRYDEPEFIVRNLYR
                     LYGGWWDGNPAHLKPPRDAALARELADLAGGAAALAARAETTADPRLMCELVELAYLA
                     APDDPAIAAIRTKIYTARAASERSVMARSVFTAAAAESQK"
BASE COUNT          446 a         1039 c         1021 g          393 t
ORIGIN      
        1 gaactcggtc agccggtcga ggccggtgag cgtgagcggc tcgtgcctgc cgagctcgcg
       61 cagcacgcct ggcctggtcg tcccgaagtg cgccagcgtc gagagccgct ggacaccgtc
      121 gatcacctcc caggtgccgt ccgtgttcgc ggccacgaag atactcggga cggcaaatcc
      181 gaggaacagc gactcgatga agcgggactg gctgcccgtg tcccagtgga gctcgcgttg
      241 atgctgcggt gctctgacca gcgctccgtc ctcgaacatg cggagcagct cgcggagcgt
      301 gaggtcgaag tgttccgcat cgacggtgcg tcgctgtgac ttgagttgcc gtgtaaggtc
      361 actggccatg atgtgcgtgg cgtcgctcaa cgacgtccac gcttgtcccc gccgcgccgc
      421 gggcgaaatc cgcggaatgc gatcacgcgc gagcggcgca gcgcgcacaa cacggcgagc
      481 acccgctccg gcgtgacgcc ggaacgcgcg agcgagcgct ggatgtcgtg gccggtgcgc
      541 ttgccgtcga tctgcgagat caccgcgagc tgctcggcgg gcacgcggac cgtcgtctcg
      601 ccccacgggg tcacgacgac gaagccgatc tcgccgtccg gtgcgtcgag ctgcagcttc
      661 gccccggtga cgggcagctc gacgatcgcg cggcgcgccg cgagcaccgg cacgagatcg
      721 agcggcgact cgtagcccgg cttcttcacg aacaagcgat gctcgacgac gacgaagtcg
      781 agccccgtcg cgaggaagca ccacagcgcg tcctccggcg tctcgacgat cggctcgccc
      841 atcacgttga aggaggtgtt cagcagcatc ggaacgccgg tctgcgcgtg gaacgccgag
      901 atcagcgcgt ggagctcggg gacgtcgcgc gcgacggtct gcagccgccc cgtgccgtcg
      961 acgtgcacga ccgcgggcac gcgcgcgcga acgtgctcgt gaaacggcac gacgcgcagc
     1021 atgaacgggc tctccgccgt gggtccgagc tcgaaccagt ccgccgcatg ctcctcgagg
     1081 atcgccggcg caaacggacg aaacgcctcg cgatgcttca cccggcgatt gagcacctcc
     1141 ttgccattcg cgagccgcgg atcggcgagg atgctacgct gtccgagcga gcgtggcccg
     1201 agctccgagc gcccgacaaa ccacccgccg agatcgccgc gcgcgagccg cttggccgca
     1261 gcgccgaccg catcgggccc cgcgacgacc tcgcggatcg cgggcgtggc gcgcacggcg
     1321 gcggacacgt cggccgcggt gtacggcgct ccgaccgcgt cgtgccggac gcgccgcgcg
     1381 cggtaggtgc tggtgaccgc gcgcaggcat tcgtacgcgg cgccgatcgc gacgccgctg
     1441 tcctcggcgg ccgccggaat gtagacgtgc tcgaagccca cctctcggat caaccgctcg
     1501 ttcgcgacgg agttcagcgc gacgccgccc gcgaagcaca ggttgcgaga gccggtcagc
     1561 gcgcgcgcac gccgggcgag ccccagcagc gcgtgctcga gcgcactctg cacggaagcg
     1621 gcgagatctc ggtacgcgcg ctcgcgctcc ggccagcgct tgccgagcac gaaccgatcc
     1681 gccgtcgcgt cggagaacac gagatcgtcg ccgtcgaacc gcaagaacgt gtcgacgggc
     1741 gcggtcgcgc ggccgtacgg tgcgagtccc atcaccttgc cggcttcgag cgcatcgccg
     1801 aacagctgga ccgcgaccgc cgagtacatg ccgccgagcg acccgaacct cggcatccgc
     1861 ttcggccggc gctgcagcca gcgtctgtcg ggcgcgaggt gcttctcgag gggcacgatc
     1921 gcatcgccgt ctgccccgta gatcgagatc gtctcccacc cgctcgggtc ggccttgcga
     1981 cacacggctc gctcgccggc gtcgaggtcg cgccacggcg aaccgagacc gtcgacaacg
     2041 agcacggccg catcggcgaa acccgacatc gagaacgcgc tcaccgcgtg cgcgcgatgg
     2101 tgagcgacgc gcaacggcgg cgggcccgcg tatccggcga gatctggatt cgcgacgagc
     2161 tgttgctccg gcgccgacgc gtagccttgc accgagatcg cggtccccgc gagtgcctcc
     2221 ggcgcgatgc cggcggcgcg caggcaatac ggaaccgcga ggctcgagcg cgagccatac
     2281 acccgggcgc gcttttcgcg cgtcaagcgt tcctcctgga tcgccacgac gatctcccgg
     2341 ccgcgcagca cgcacacagc gccgttgtgc gaggcgctca taccgaggat caagggctcg
     2401 gatgccgtca tcgatcgatc tcatacgcca ggactggacg tttcgccacc gaaccagttc
     2461 gggccgcgat catttctgcg actcggccgc cgccgcggtg aacacgctgc gcgccatcac
     2521 cgaccgctcc gaagcggcgc gcgccgtgta gatcttcgtg cggatcgcgg cgatcgcagg
     2581 gtcgtccggc gcggcgaggt acgcaagctc gaccagctcg cacatgagcc gcggatcggc
     2641 ggtcgtctcg gcgcgggcgg ccagcgcggc ggcgcccccg gcaagatcgg cgagctcgcg
     2701 cgccagcgcg gcgtcgcgcg ggggcttgag gtgcgcgggg ttgccgtccc accagccacc
     2761 gtagagccgg tacaggttgc ggacgatgaa ctcgggctcg tcgtaccttg gcgacaggta
     2821 cggccgggcg gcgagcgcgg ccgggggcgc gacgctcgcg atcacctcgt cgagccgcgc
     2881 gccgcggttc atcagcgtc
//
LOCUS       DASVOR010001450         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_453004,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001450 DASVOR010000000
VERSION     DASVOR010001450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453004"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VF516_27520"
     CDS             <1..217
                     /locus_tag="VF516_27520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111523.1"
                     /translation="VLRHIRSAARALVAGGLTFGALELSVQGTLAVFGAPALGVGTRV
                     ILAGAAAAIGLGWQTLRLGDRLRRRIG"
     gene            238..1242
                     /locus_tag="VF516_27525"
     CDS             238..1242
                     /locus_tag="VF516_27525"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111524.1"
                     /translation="MESGSMIGGRYQLVDVVAEGGLSTVWQGVARSSEVFARPVAIKV
                     MKRAFSTVGGPYLSMFLEEARIGARLQHSNVIQVLDFIVEATPAGPTYCLVLEWVDGI
                     DLRSLLRVENHLGRKLAWGLVATVGLRVLRGLAAAHERRLPDNTLSPVIHRDVSPQNI
                     LLGLNGDIKLADFGMARARDRIAEHTAPGFVKGTLSYMAPEILVGKPASPASDLFSLA
                     ITLWEALAGERLFHAKSDIDVIEGIRRCEVPPLADRRPDVPPALVAVIHRALAKEPEA
                     RFVSARQMAHELNEILRGEDSWGDADVLVGNAVAEARVGQAQLPPAPPAPGSSSGSSR
                     "
     gene            1436..1735
                     /locus_tag="VF516_27530"
     CDS             1436..1735
                     /locus_tag="VF516_27530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111525.1"
                     /translation="MSEPELDDGVRDLLETRLDSFEKLEIAHALRASGGMTPEALEAT
                     CRFSAETVPATSGLLLYDNHASVARLAPLRDPAARLSRDRRSDPGQEPAAVTEPR"
BASE COUNT          240 a          640 c          635 g          268 t
ORIGIN      
        1 cgtgctccgc cacatccggt ccgccgcgcg ggcgctggtc gccggcggcc tcacattcgg
       61 cgcgctcgag ctgtccgtgc agggcaccct ggcggtgttc ggcgcacctg cgctgggcgt
      121 cgggacccgg gtcatcctcg ccggcgcggc cgcggcgatc ggcctgggct ggcaaacgct
      181 ccggctcggc gaccggctgc gccgccggat cggctgacaa tcggctacca tcgggtcatg
      241 gaatccggat cgatgatcgg aggccggtac cagctcgtcg acgtcgtcgc cgagggcggc
      301 ctgtccacgg tctggcaggg cgtggcgcgc agcagcgagg tgttcgcccg tccggtcgcg
      361 atcaaggtga tgaagcgggc gttctcgacg gtcggcggcc cctacctgtc gatgttcctc
      421 gaggaggccc ggatcggggc gcggctgcag cactccaacg tgatccaggt cctcgacttc
      481 atcgtcgagg ccaccccggc cggtccgacc tactgcctgg tgctggagtg ggtcgacggc
      541 atcgacctgc gcagcctgct ccgggtcgag aaccacctcg gccgcaagct ggcctggggc
      601 ctggtcgcca cggtcggcct gcgcgtgctg cgcgggctgg ccgcggccca cgagcgccgg
      661 ctgcccgaca acacgctgtc gccggtgatc caccgcgacg tgtcgccgca gaacatcctg
      721 ctcgggctca acggcgacat caagctcgcc gacttcggca tggcgcgcgc gcgcgaccgg
      781 atcgccgagc acaccgcgcc cgggttcgtg aagggcacgc tgtcgtacat ggcgcccgag
      841 atcctggtcg gcaagccggc gagcccggcc agcgacctgt tctcgctggc catcacgctg
      901 tgggaggcgc tggccggcga gcgtctgttc cacgccaagt ccgacatcga cgtcatcgag
      961 gggatccggc gctgcgaggt cccgccgctc gccgatcgcc ggcccgacgt cccgccggcg
     1021 ctcgtcgccg tgatccaccg cgcgctggcg aaggagcccg aggcgcggtt cgtctcggcg
     1081 cgtcagatgg cgcacgagct caacgagatc ctgcgcggcg aggactcgtg gggcgatgcc
     1141 gacgtcctgg tcggcaacgc ggtcgccgag gcgcgggtcg gccaggctca gctgcccccg
     1201 gccccgccgg cgccgggctc cagctccggc agctcccggt agccgggcca ccgagcgctg
     1261 gcgcacatcg tccacggcga cccctgcgcg tacgcgattc ctcgcacgcg atgcgccagg
     1321 cgcgctctgc atcgcgagct gcagactggc aatgacgatc gtttgagcac aaacgatcgt
     1381 cggtccggct tgtggcggaa tgccccacct gatatagctg tcggaggttc gttggatgag
     1441 cgagccagag ctcgacgacg gggtacggga cctgctggag acccggctcg actcgttcga
     1501 gaagctcgag atcgcgcacg cgctgcgcgc gtcgggcggc atgacgccgg aggcgctgga
     1561 ggcaacgtgc cggttctcgg cggagaccgt ccctgccacc tcgggcctgc tgctgtacga
     1621 taaccacgcg tcggtagcac gcctcgcacc tctacgcgat ccggctgctc ggctttctcg
     1681 cgatcgtcgc agcgatcctg gtcaagaacc ggcggcggtg accgaaccgc gctgatccgc
     1741 gatagcagtg cgggtcgctc gcagccccgc ggagcactgg acc
//
LOCUS       DASVOR010001451         5186 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_15652,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001451 DASVOR010000000
VERSION     DASVOR010001451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15652"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..949
                     /locus_tag="VF516_27535"
     CDS             <1..949
                     /locus_tag="VF516_27535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111526.1"
                     /translation="VSPFDFAFFARRVNTIFDDINAVEQGAQGRSCSDTTACHGVAVA
                     GQRPPNGSNFPILPNAADLGRLTANFASATGFATFLNPGESSLFLYPTNEIADRNAHP
                     FATGFAHPGGADFAVDSAEAKAILQWAAGLRPDANGFQRNWLVVGDFPATRVADTTLV
                     NETTVTPKIFDAAGGSFNNGQWDGLFSDRKSVDLNSAFPRAATSGRVAYAVSYALNTT
                     PRQVDALLFVATQNPIRIYVNGLIAGQNDQPGGASASFSLPPAGPGQKPARILIKLLQ
                     QSTDASFAFTAQLTDANVGTQLTDTTGELVFTLGPGGGF"
     gene            949..3858
                     /locus_tag="VF516_27540"
     CDS             949..3858
                     /locus_tag="VF516_27540"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX8111527.1"
                     /translation="MFRHVIVMLALTACQDSLVTGGDPSPGGTGSGGTTVPPPDKPPV
                     TQQPAAYKRGSLQPLYQLTPRDEYGRFIQNGVQMADTDFISAANNFVSAAQKMDEIGG
                     QLAADNGATTTLDLVNRDRDRAVQIPFRGNPSDVKVVRINGLRKAYVPLGGDLMSPGN
                     EVASVDLATGAVTRIKVGIRPQRVAVHPDGLVFVCNQFSNYISIIDPRTDRLLSNGQG
                     PIEIKTEYYCTDLTFVPRNPAAPNADLQDLYVANSWRGSVLQYGLTVTRDQLGNTPVD
                     VRVTSPAKPSPENQPAAEITGVGSNPYRLSVSQDQQAIYVANNRGGELARFDIASKAV
                     NRIAINAPVPDIAQANDIVVVPTTMIDRGLPDAQDPQPTQISAPAVRVTGLDGQQHVA
                     HPGALFDHTKAYNFEDMRNGLLTIDAQLPANTVPTYFTDDISAEPNFTANQKVLAGSL
                     PQAIAINAAHNRAYLAMSGSDNVQVVSIQGGIFRIAKSGQALIQTAKRPFALTLDEAA
                     GELLVAAWGGEVLQTFSISTGALKKTIDLGYATAAYPATNMERGEYLYYNTAWSNNGR
                     KSCATCHFEELLLDGIPYANGATAPTAYHKVPSNWNLLTTNSYFWNGSFGNGTYASLA
                     SDAQTRTNCELIAFGETEGIASNPATRVGDPANRVRSAQDTQCRPDTSNNSVLPANFA
                     QIQSIIAADHLVRDQVVKAATAATVNVAGGLGFEDVARLVDFYSVSELRLPPNPLAFL
                     SKNKLLSAADQDAITRGKGVFQSAGCINCHDANNQRHPFTDGQNHGSGTMWLTNFINT
                     YGQDSRILAIGGIPSLMAEANVASTPDKEINIHLDPIDFFEPFCFDLNHCLVFDDPLA
                     VRGNKAAEDVRLNALIQINLANVDRGFVPGNVRGSPLSNTPSLRGIWFQTNFLRHGLA
                     HTFREAVLAPGHPALGPGELGFAIDALGNKDVHGATSKLSSADLDALFQYVQTIE"
     gene            3871..4092
                     /locus_tag="VF516_27545"
     CDS             3871..4092
                     /locus_tag="VF516_27545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111528.1"
                     /translation="MMSERSTLTYVRRAFTKVKRELVDEFERRYLLDLLGRYQGDLSA
                     VRRASGLSRRHLGALLAKHGLTDHVARGR"
     gene            4240..>5186
                     /locus_tag="VF516_27550"
     CDS             4240..>5186
                     /locus_tag="VF516_27550"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111529.1"
                     /translation="MNIGARRDAGNHSRSQRTAERTTRPPGGSRLRERFGLYTLHERL
                     GAGGTCAVFAAEHPVLRGPFAIKILRRKLLGDSTITRRMLDEASMIARLDHPGIVEVY
                     DFGCSTAGRPFVVLERLVGEPLSRRLERGPLPEDRLVLFSGQLASALDAAHRNGIVHR
                     DLKPDNIYIVPDPASPCGERIKLLDFGIAKFRASTCVTGPGIVLGTPGYMAPEQARGT
                     RQLDGRADIYALGVVMYVMATGAPPFTGTNTGEILAAHEYCEPVPPAELAYITPELSA
                     IIQRCLAKRPEHRYPGVRDISDALAGVEPRRRGVPVVATP"
BASE COUNT          854 a         1912 c         1668 g          752 t
ORIGIN      
        1 tgtctcgccg ttcgacttcg cgttcttcgc gcgccgggtc aacacgatct tcgacgacat
       61 caacgcggtc gagcagggcg cccagggccg cagctgctcg gatacgaccg cctgccatgg
      121 cgtcgccgtc gccggccagc ggccgccgaa tggctcgaac ttcccgatcc tcccgaacgc
      181 ggccgatctg gggcgcctca cggccaactt cgcctcggcg accgggttcg cgaccttcct
      241 gaatccgggc gagagctcgc tgttcctgta ccccaccaac gagatcgcgg accgcaacgc
      301 gcacccgttc gcgaccggct ttgcgcatcc cggcggcgcg gacttcgccg tcgactcggc
      361 cgaggcgaag gcgatcctgc agtgggcggc cgggctccgg cccgacgcca acgggttcca
      421 gcgcaactgg ctggtcgtgg gcgacttccc cgcgacgcgg gtcgccgata cgacgctggt
      481 caacgagacc acggtgaccc cgaagatctt cgacgccgcg ggcggctcgt tcaacaacgg
      541 tcagtgggac ggcctgttct cggaccgcaa gagcgtcgat ctgaactcgg cgttcccgcg
      601 cgccgccacc agcggccgcg tcgcgtacgc ggtctcgtac gcgctcaaca ccacgccgcg
      661 gcaggtcgac gccctgctgt tcgtcgcgac ccagaacccg atccggatct acgtcaacgg
      721 cctgatcgcg ggccagaacg accagcccgg cggcgcgagc gcgagcttca gcctgccgcc
      781 ggccggcccc ggccagaagc cggccaggat cctgatcaag ctgctgcagc agtcgaccga
      841 cgcgagcttc gcgttcaccg cgcagctcac cgacgccaat gtcggcacac aactcaccga
      901 caccacgggc gagctcgtct tcaccctcgg ccccggagga ggtttctgat gtttcgccat
      961 gtcatcgtca tgcttgctct caccgcgtgc caggacagtc tcgtgacggg cggcgatccc
     1021 agccccggcg gcaccggcag cgggggtacg actgtgccgc cgccggacaa gcccccggtc
     1081 acgcagcagc ccgccgcgta caagcgcggg tcgctccagc cgctgtacca gctcacgccg
     1141 cgcgacgagt acggccggtt catccagaac ggcgtgcaga tggccgacac cgacttcatc
     1201 tcggccgcga acaacttcgt ctcggcggcc cagaagatgg acgagatcgg cggccagctc
     1261 gcggccgata acggggccac gacgacgctc gatctggtga atcgggatcg cgatcgtgcg
     1321 gtgcagatcc cgttccgcgg caatccgtcg gacgtcaagg tcgtgcggat caacggcctg
     1381 cgcaaggcct acgtgccgct cggcggcgac ctgatgtccc cgggcaacga ggtggcctcg
     1441 gtcgatctcg cgaccggcgc ggtcacccgg atcaaggtcg gcatccgtcc ccagcgcgtc
     1501 gcggttcatc ccgacggcct ggtgttcgtg tgcaaccagt tctcgaacta catcagcatc
     1561 atcgacccgc gcaccgaccg cttgctgagc aacgggcagg ggccgatcga gatcaagacc
     1621 gagtactact gcaccgatct cacgttcgtg ccccgcaacc cggcggcacc gaacgcggac
     1681 ctgcaggacc tctacgtcgc gaacagctgg cgcggcagcg tgctccagta cggcctgacc
     1741 gtcacgcgcg accagctggg gaacacgccg gtcgacgtgc gagtcaccag cccggcgaag
     1801 ccgtcgccgg agaaccagcc cgcggccgag atcaccggcg tcggctccaa tccttaccgg
     1861 ctcagcgtct cccaggacca gcaggcgatc tacgtcgcga acaaccgcgg cggcgagctc
     1921 gcgcggttcg acatcgcgag caaggcggtg aaccggatcg cgatcaatgc cccggtcccc
     1981 gacatcgccc aggccaacga catcgtggtg gtcccgacga cgatgatcga tcgcggcctg
     2041 cccgacgcgc aggacccgca accgacgcag atcagcgcgc ccgccgtccg cgtcaccggg
     2101 ctcgacggcc agcagcacgt cgcgcacccc ggcgcgctgt tcgaccacac caaggcctac
     2161 aacttcgagg acatgcgcaa cggcctgttg acgatcgacg cccagctgcc ggcgaacacc
     2221 gtgccgacgt acttcaccga cgacatctcg gccgagccca acttcacggc gaaccagaag
     2281 gtcctggcgg gctcgctgcc ccaggcgatc gcgatcaacg ccgcccacaa ccgcgcgtac
     2341 ctcgcgatgt ccggctcgga caacgtgcag gtggtctcga tccagggcgg catcttccgg
     2401 atcgcgaaga gcgggcaggc gctgatccag accgcgaagc ggccgttcgc gctcacgctc
     2461 gacgaggcgg ccggcgagct gctcgtcgcg gcctggggcg gcgaggtgct ccagaccttc
     2521 agcatctcga ccggcgcgct caagaagacc atcgatctcg gatacgcgac cgccgcctac
     2581 cccgcgacca acatggagcg cggcgagtac ctgtactaca acacggcgtg gtcgaacaac
     2641 ggccgcaagt cgtgcgcgac ctgccacttc gaggagctct tgctcgacgg gattccctac
     2701 gcgaacggcg ccaccgcgcc gaccgcctat cacaaggtgc cctcgaactg gaacctcttg
     2761 accaccaact cctacttctg gaacggctcg ttcgggaacg ggacctacgc gtcgctcgcg
     2821 tccgatgccc agacccgcac gaactgcgag ctgatcgcgt tcggcgagac cgagggcatc
     2881 gcgtccaatc cagcgacgcg cgtgggcgac ccggcgaacc gggtccggag cgcccaggac
     2941 acgcagtgcc ggcccgacac ctcgaacaac agcgtgctgc cggcgaactt cgcgcagatc
     3001 cagtccatca tcgcggcgga ccacctggtg cgcgaccagg tcgtgaaggc cgcgaccgcg
     3061 gcgacggtca acgtggccgg ggggctgggg ttcgaagatg tcgcacggct ggtcgacttc
     3121 tactcggtgt ccgagctgcg gctgccgccg aacccgctgg ccttcctctc gaagaacaaa
     3181 ctgctcagcg ccgccgacca ggacgcgatc acgcgcggca agggcgtctt ccagtcggcg
     3241 ggctgcatca actgccacga cgcgaacaac cagcgccatc cgttcaccga cgggcaaaat
     3301 cacgggtccg gtacgatgtg gctcaccaac ttcatcaaca cctacgggca ggattcgcgg
     3361 atccttgcga tcggtgggat cccgtcgctg atggcggagg cgaacgtggc gtcgactccg
     3421 gacaaggaga tcaacatcca tctcgatccg atcgacttct tcgagccgtt ctgcttcgac
     3481 ctcaaccatt gcctcgtgtt cgacgatccg ctggccgtcc gcggcaacaa ggccgcggag
     3541 gacgtgcggc tcaacgcgct gatccagatc aacctcgcga acgtcgaccg tgggttcgtc
     3601 ccgggcaacg tgcgcggtag cccgctgagc aacacaccgt cgctgcgcgg gatctggttc
     3661 cagaccaact tcctgcgcca cgggctcgcg cacacgttcc gcgaggcggt cctcgcgccc
     3721 ggccacccgg cgctcggccc cggggagctg ggcttcgcga tcgacgcgct cggcaacaag
     3781 gatgtccacg gcgcgacgtc gaagctcagc tccgcggacc tcgatgcgct gttccagtac
     3841 gtccagacca tcgagtgagg agagccgctc atgatgtccg agcgctccac cctgacctac
     3901 gtccgacgag cgttcacgaa ggtcaagcgc gagctggtgg atgagttcga gcgccgctac
     3961 ctcctcgatc tgctcggcag gtaccagggc gatctgtccg cggtccggcg agcctcggga
     4021 ctttcccgac ggcaccttgg cgcgctgctc gcgaagcacg gcctcacgga ccacgtcgcg
     4081 cgtgggcggt gaatccccac tccggcggca gctccggggg actcaccccc accccggggc
     4141 tgcccgcccg cgcgcgggtg cggcgtggcc atccgaggaa cagcgcgctg gctggaccgg
     4201 ttcttgcctt cgttcgccca ccgtggtcag gagggaacca tgaacatcgg cgcacgccgt
     4261 gacgccggaa accattcgcg ctcgcagcgt accgccgagc ggacgacgcg cccgccgggc
     4321 ggatcccgcc tgcgcgaacg cttcgggctc tacacgctgc acgagcggct cggtgcgggc
     4381 ggcacctgtg ccgtgttcgc cgccgagcat cccgtgctgc gcgggccgtt cgcgatcaag
     4441 atcctgcgcc gcaagctgct gggcgattcg acgatcacgc ggcggatgct cgacgaggcg
     4501 agcatgatcg cgcggctgga tcatcccggg atcgtcgagg tctacgactt cggatgctcg
     4561 acggcgggcc ggccgttcgt cgtgctcgag cgcctggtcg gcgagccgct gagccggcgg
     4621 ctcgagcgcg ggccgctgcc cgaggatcgc ctcgtgctct tctccgggca gctcgcctcg
     4681 gcgctcgatg ccgcgcaccg caacggcatc gtccaccgcg atctcaagcc cgacaacatc
     4741 tacatcgtcc cggaccccgc gtccccgtgc ggcgagcgga tcaagctgct cgatttcggc
     4801 atcgccaagt tccgggcgtc gacctgcgtc accggccccg gcatcgtgct ggggacgccg
     4861 gggtacatgg ctccggagca ggcgcgcggc acgcgtcagc tcgacggccg cgccgacatc
     4921 tacgcgctcg gcgtcgtgat gtacgtgatg gcgaccggcg cgccgccgtt caccggcacc
     4981 aacaccggcg agatcctcgc cgcgcacgag tactgcgagc cggtcccccc ggccgagctg
     5041 gcgtacatca cgcccgagct gtcggcgatc atccagcgct gcctcgcgaa gcggcccgag
     5101 catcgctacc cgggggtgcg cgacatctcg gacgcgctcg ccggcgtcga gccccggcgc
     5161 cgcggcgtgc ccgtcgttgc cacgcc
//
LOCUS       DASVOR010001452         5728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_540931,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001452 DASVOR010000000
VERSION     DASVOR010001452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5728
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_540931"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..142
                     /locus_tag="VF516_27555"
     CDS             <1..142
                     /locus_tag="VF516_27555"
                     /inference="COORDINATES: protein motif:HMM:NF021902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase"
                     /protein_id="HEX8111530.1"
                     /translation="VATPSDWKQGQDVIIVPSVSDADAKAAFPSGWKTLKPYLRLVPQ
                     PK"
     gene            complement(454..2421)
                     /locus_tag="VF516_27560"
     CDS             complement(454..2421)
                     /locus_tag="VF516_27560"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8111531.1"
                     /translation="MVGQPKLERRSGPAWSGAPRPELLPGMGPLRMGGAPWVAQARTN
                     RGVSTTPIQVGQLRVIGEGRPLEPGIRQIMESFFQAEFSGVRVHEGPAAPALGALAFT
                     LGEDLHFAPGLYEPGSREGLALLGHELTHVVQQRDGRVANPYGQGIASVQDPVLEAEA
                     DAMGHRVADHVHSQLLARSPRKVVQQRIGPLSRFGRGVPGTVPLAPDRRAAGSDGRGG
                     LGPSRGAHPFSPEREAVRPDTGGVVQWMKRRRSEGEIPVRKSDEGEERDDTETEEEKE
                     KTPKEIEEENFKQLREILTLNAFGGPQAGYVVNGTKDSAPGSPPRPYKGSRHIQVLRH
                     IGIAVYVLARRGSGAKTDKIVEVQSMFAGGNLYIATNNAPAARWLCDVLNSDWATTYA
                     GLDVVKLDQRWNLMTAEVTKRVIGRIKKRIALYQKNEFTFTTFDSSDEAERSSLEIAK
                     QIWKVARSKNCRIISSEEEFKDSLTKKGKIYVVMDDSQLHAEMNFLPIIDLMDPNELK
                     LVAGKKRQCFTCFVRLAMRKDELPPGTLITQTTSPGNLWKNQLGFIKDGEATNFMGWC
                     EKHSDIKSVVTVLNYEPDKSYIMIGSQSESESESESESDTGEKESPKKKSRTAEPELS
                     EEDDDADVLLEELGIEDEDEGKVATGEKEEK"
     gene            3136..5112
                     /locus_tag="VF516_27565"
     CDS             3136..5112
                     /locus_tag="VF516_27565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111532.1"
                     /translation="MPALDLQMVSSTSAKATGARSWASASTFCVPVMLVAACFQPSYH
                     HPTCGPREECPSGLMCIEHVVCGEPDSQPGTWGFASAADFTAPGYAMASMTIEPSGLL
                     TPTAYTYGGLMAHGLPGMKLWSHGDTSWTKLDAVTAAGAGLWRGESFTTTSVLSYLGI
                     TDDKQAAPATLWFEGEVWLDAGSAEQFELTANDIAFVELARPGTSDYVPLTESSAAVP
                     VTAPDTGWYPIRIGVASADRSMLSFAFLHGEGSAPLVPWTRDRMRARASEVSGALRLV
                     FANQVLGGGQDTPAPIGHIEDGDLLPFTTFPVAPQGASGVTNWSARYIGQVYADQPGS
                     YTLTVSSDDGNRARLGSASAQTSWGFNAGNSAAVTTVPVTLNVGWNDLAIDYNQLTGG
                     ALLQATIDGPDLVEVPVPRDRLRPVESADDRLAFGGDDAQYMIPLGGGSSQPATATMS
                     VAAYAGEVVTSIDVAYVVTENSPLIANADLETGNTRLNLGVAGRILTGTDDLLFQVTV
                     PPGAGPLAQLLGGPAGGIWKLHVYDDRLGDKGIPSVLKSARLTLHTTGGPERIARTAS
                     WTSPLLALPSDVLLVSNVMWDARLPQGATVSVQARSCAQPDCSHAAWLPVTPSAATAL
                     PPAPFLQLRLELTSDGVVEPELRSLSVSYVRTPG"
     gene            complement(5292..>5728)
                     /locus_tag="VF516_27570"
     CDS             complement(5292..>5728)
                     /locus_tag="VF516_27570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111533.1"
                     /translation="RLARERAAAAASSPSGTPGATPPQDTSSPEAAAPPGRNLRIAGM
                     ATGAGGVVGVAIGIAFGLHARTLSNELSNTGVMYDPSKVDAGQRANTIAIAGMAGGTA
                     LVATGAALYWWGYMQGRSTERVSLAPILSERLTGLAIAGTWR"
BASE COUNT          882 a         2049 c         1797 g         1000 t
ORIGIN      
        1 ggtcgcgacc ccgtcggact ggaagcaggg ccaggacgtg atcatcgtcc cgtcggtgtc
       61 ggacgccgac gccaaggccg cgttccccag cggctggaag acgctcaagc cgtacctgcg
      121 cctcgttccg cagcccaagt agccggcgcc cggacctact tcgcgcgggc cgcgaaccat
      181 cgactcggcg agggcggacc gcccggcgcc ggcgcaggtg ccggtcgaac accgcgatgc
      241 gccgcagccc gccgtacatc atcgcggccg gccgcgacca ggctgtcaag ccagcgcgct
      301 ccaccggcct gtcgcgatcg tacgccgtgc gacaccccgg gccacggagc cgccgctccg
      361 cacgacctgg agccgctccg gtgtcctctc ggcctcggtc gtcttgccgc gcgtctcggt
      421 gaatcgcatc gcctcgggta ggctgccacg agctcatttc tcttccttct ctcccgttgc
      481 caccttcccc tcgtcctcgt cctcgatacc gagctcctcc agcagcacat cggcgtcgtc
      541 atcttcttcg ctcagctccg gctcggccgt gcgtgacttc ttcttggggg actccttttc
      601 ccctgtatcg gactccgact ccgactccga ctccgactcc gactgcgagc cgatcatgat
      661 ataagatttg tcgggctcgt aattcagcac cgtcacgact gacttgatgt cagagtgctt
      721 ttcgcaccag cccatgaagt tcgtggcttc gccatctttg atgaagccaa gctggttctt
      781 ccacaggttg cctggactcg tcgtctgggt gatcagcgtg ccgggtggga gctcgtcctt
      841 ccgcatcgcc agccgcacga agcaggtgaa gcactgacgc ttcttgccgg cgacgagctt
      901 gagctcgttc gggtccatga ggtcgatgat cggcaggaag ttcatctcgg catgcaactg
      961 gctatcgtcc atgaccacat agatcttgcc ttttttggtc agtgaatcct tgaattcttc
     1021 ttcactgctg atgatcctgc agttcttgct cctggctacc ttccagatct gctttgcaat
     1081 ctccaaggag ctcctctccg cttcgtccga ggagtcgaag gtagtaaacg taaattcgtt
     1141 cttctggtag agcgcgatgc gcttcttgat ccgcccgatg acgcgcttcg tgacctctgc
     1201 cgtcatcaag ttccatcttt gatcgagctt tacgacatcg agaccggcat atgtagtcgc
     1261 ccaatcgctg ttgaggacat cgcagagcca ccgggcggcg ggggcgttgt tcgtggcgat
     1321 gtagaggttg ccgccggcga acatgctctg gacctcgacg atcttgtcgg tcttcgcccc
     1381 ggatccgcgc cgcgcgagca cgtagacggc gatgccgatg tgtcgtagaa cctggatgtg
     1441 ccggctgccc ttgtacggcc tcggcggaga cccgggggct gagtccttcg tcccgttcac
     1501 gacgtaacca gcctgtggcc cgccgaaagc attgagcgtc aggatctccc ggagctgctt
     1561 gaaattctcc tcctcgatct ccttcggcgt cttctccttc tcctcctcgg tctcggtgtc
     1621 gtcgcgttcc tcgccctcgt cggacttccg aactgggatc tccccctcgg agcgcctccg
     1681 cttcatccat tggaccacgc ccccggtgtc cggcctcacc gcctcccgct ccgggctgaa
     1741 cggatgggcc ccgcgcgatg ggcccagccc gccgcgccca tcgcttcccg cggcacgccg
     1801 atccggcgcg agcgggaccg taccggggac cccgcgcccg aagcggctca gcggtccgat
     1861 cctctgctgg accacctttc gcggtgagcg cgccaggagc tgggagtgca cgtgatccgc
     1921 cacccggtgc cccatcgcgt ccgcctccgc ttccagcacc ggatcctgga cgctcgcaat
     1981 tccctgccca tacggattgg caactcgccc atcccgctgc tgcaccacat gtgtcagctc
     2041 atgccccagc agcgccaacc cctctcggct ccctggctca tacagccccg gcgcgaagtg
     2101 cagatcttcc cccagggtga acgccagcgc ccccagcgcc ggtgccgcgg ggccctcgtg
     2161 cactctcacg ccagagaact ccgcctgaaa gaagctctcc atgatctggc gaatccccgg
     2221 ctcgagtggc cttccctcgc cgatcactcg cagctgcccg acctggatcg gcgtcgtcga
     2281 gacgccccgg ttggttcggg cctgcgccac ccatggcgct ccccccattc tcaacggccc
     2341 catccccggc agaagctcgg gccgcggcgc cccgctccac gccggtccag accgccgctc
     2401 gagcttgggc tgcccgacca cgctccgcat cctggccggc aacagctcgg gccgtggcac
     2461 cctggatgcc ctcggccccg gatggggttg cgcggtcgcg ccccgcatgc tcaccggcag
     2521 gaactcgggc cgcggtggtg acggaatcgg tcgccccgga tgcaccgact gcgccgctgc
     2581 ggccgtggtt ctcgcagccc gaaacggctc gatacgaaag tacccctgca ataccaggcc
     2641 agccttcgat cctgtccgct tcaaggtcac gatgtctccc gacgagccgt catcgagatc
     2701 tatccaaccg gggcactcgc tcgccagttt ctggcacccg cacgccgatc accctgcgcg
     2761 cgcctcgatc ccggcccgcc gcgcaggtcg cgccgcggcg ccgtgggtca aagcgccgcg
     2821 gcggccccta ccacagcggc ccgctggctc acaaaagcat cgcgaagtgc atcagtccta
     2881 acacaccgct gaagcggaga tatgtaggag ctgcgcccag caaacccgat gacacggaca
     2941 ggccctatcg aagtccgtgg agcgtaccat gcctggcgag atccaagacg ggggcacccg
     3001 agagtcgaag attctgatcc aggcgtcgcc tgttggcgcg cgcccttctg gagggaactc
     3061 catgctggac gacgatccgc cgctgttgca cattggccat ggtcccgcgg ctcgtagcca
     3121 gatcacggct atcgattgcc cgcgctggac ttgcagatgg tgagcagcac atccgcgaag
     3181 gcaacaggag cgcgctcctg ggcatccgcc agcacattct gcgtgcccgt gatgctcgtc
     3241 gcagcgtgct tccaaccgag ctatcaccac ccgacgtgcg gccccaggga agaatgtccg
     3301 agcggcttga tgtgcatcga gcacgtggtg tgtggcgagc ccgacagcca gccagggaca
     3361 tggggctttg cctcggcggc ggacttcacc gcgccgggct atgccatggc cagcatgacg
     3421 atcgagccga gcggcttgct gacgccgacc gcctacacct atggcggcct gatggcgcac
     3481 ggcctgccgg gcatgaagct gtggtcgcac ggcgacacca gctggaccaa gctcgacgct
     3541 gtgaccgcgg cgggtgcagg tctgtggcgc ggcgaatcat tcaccacgac cagcgtgctg
     3601 tcgtacctgg ggatcaccga cgacaagcag gccgcgcctg cgacgctatg gttcgagggc
     3661 gaggtctggc tcgatgctgg ctcggccgag cagttcgagc tcacggccaa cgacatcgcg
     3721 ttcgtcgagc tcgcgcggcc cggaacatcg gactatgtcc cgctgaccga gagcagcgcc
     3781 gcggttcccg tcacagcacc ggacaccggc tggtatccga tccgcatagg cgtcgcaagc
     3841 gccgaccgct cgatgctgag ctttgcgttc ctgcacggtg aaggaagcgc gccgctggtg
     3901 ccgtggaccc gcgatcgaat gcgagcgcgc gccagcgagg tctccggcgc gctgcgcctg
     3961 gtcttcgcca accaggtcct cggcggcggc caggacacgc cggctccgat cggccacatc
     4021 gaggacggcg acctgctgcc gttcaccaca ttcccggttg cgccgcaggg cgcatccggg
     4081 gtgacgaact ggtccgcgcg ctacatcggc caggtttacg ccgatcagcc aggctcgtac
     4141 acgctgaccg tctccagcga tgacggcaac cgggcacggc tcggcagcgc ttcggcacag
     4201 acaagctggg gattcaatgc aggcaacagt gccgcggtga caacggttcc ggtgacgctg
     4261 aatgtgggct ggaacgatct cgcgatcgac tacaatcagc tcaccggcgg cgccctgctg
     4321 caagccacca tcgatgggcc ggatctcgtc gaggtgccgg ttcctcgcga tcggctgcga
     4381 ccggtggagt cggccgacga tcggctcgcg ttcggcggcg acgacgccca gtacatgatc
     4441 ccgctgggcg gcggctccag ccaacccgcc acggccacca tgtccgtggc cgcctacgcc
     4501 ggcgaggtcg tcacttcgat cgacgtggcc tacgtggtca cggagaactc gcccctgatc
     4561 gcgaacgccg atctcgagac cggcaacacg cgactcaatc tcggcgtggc cggacgcatc
     4621 ttgaccggga ccgatgacct gctgttccag gtcacggtgc ctccgggggc ggggccgctc
     4681 gcgcagctgc tcggcggtcc tgcaggaggg atctggaaac tgcacgtcta tgacgatcgt
     4741 ctcggcgaca agggcattcc tagcgtgctg aagagcgcca ggctcacact gcataccacc
     4801 ggcggccctg agcggatcgc tcgtactgca tcgtggacgt cgccgctgct ggccctgccc
     4861 agcgatgtgc tgctcgtcag caacgtcatg tgggatgcgc ggcttccaca gggcgcgacc
     4921 gtctccgtgc aggcgcgcag ctgcgctcag cccgattgca gccacgcggc ctggctgccg
     4981 gtcactccat ccgctgcgac cgcgctccct cccgcgccat ttctgcagct gcggctcgag
     5041 ctgaccagcg atggcgtggt cgagccggag ttacgatcac tgagcgtgag ctacgttcgc
     5101 acaccaggct aacgcggctt tccatgtgcg ctggtcgggc cggaccgagg tcgccggcga
     5161 gatccagcgc ggcatcgagg ttatgggcgc cgaccacggc caccgcaccc agagcttcgg
     5221 cgatccgcgg cgccgcgggc atgatggggt gctggaccca gcggtgcggt tcagatcggg
     5281 cccggggcgg ctcaccgcca ggttccggcg atggccaggc cggtgagtcg ctcggacagg
     5341 atcggcgcca gcgaaacccg ctcggtcgat cggccctgca tgtagcccca ccagtacagc
     5401 gccgcacccg tcgcgaccag cgccgtgccg ccggccatgc cggcaatcgc gatcgtgttc
     5461 gcgcgctggc cggcatcgac cttcgacggg tcgtacatga cgcccgtgtt cgacagctcg
     5521 ttggacagcg tgcgcgcgtg gagaccgaag gcgatgccga tggccacacc cacgacaccg
     5581 cccgcgccgg tcgccatccc ggcgatccgc aggttgcgcc cgggcggtgc agcggcttcc
     5641 ggcgagctgg tgtcttgcgg cggcgtggcc ccgggggtcc cgctcgggga gctggcagca
     5701 gcggcagcgc gctcgcgtgc caggcgag
//
LOCUS       DASVOR010001453         8938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_44312,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001453 DASVOR010000000
VERSION     DASVOR010001453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8938
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44312"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            269..3301
                     /locus_tag="VF516_27575"
     CDS             269..3301
                     /locus_tag="VF516_27575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8111534.1"
                     /translation="MSDNDRTLFGTGASWASSQPMVTLPIGMVPRPARVAGGTPYPGP
                     RSPAAPPAPLPVPEAPGGTGQSPWDSSGDAVTAVHAISATLQPGTRIHQYEIIKMIGE
                     GGMGSVFLARDLRLGRRVAIKFLRSDEPEQTQRFLVEARTTARCQHDNIVVIYEVGEH
                     DGAPYIVLEYMNGKPLSHLTEDGQKLPYTRAVEVVIPILRALGCAHMQGIVHRDLKPD
                     NVFLTESGTIKVLDFGIAKVLQGHAGGDGEAAATRMPSPLELATGTDAGLTRVGAIMG
                     TLQYMSPEQWGIGVEIDHLTDIWACGVLLHRLICGRHPLYPLDGNQLVVTAMLDVAMP
                     SMVAATPPDVPRELIQIVDRCLLKDKAQRWQSAAELMAALEPFLPGRRTQTLQVDESP
                     YAGLSPFQESDAGKFFGRNREITAMVARIRDRPLLAVVGSSGVGKSSLVRAGLVPALK
                     RSGEPWEVLVARPGRAPLDALADVIAPMVTTAANLADEVGEHRKLVDTLRSEPGHLGH
                     VLRLRARRDNRRILLFVDQFEELYTQTTEAAERAAFTACLAAVADDATSPLRVVLSFR
                     SDFLDRVAEDPAFAGELTQGLLFVGPPGREGLREAITQPAELAGFQFELPATVEDMLD
                     HLETTPDALPLLQFTAAKLWELRDTARRLLTHRAYAAIGGVAGALASHADRFVAELGA
                     HRAPLVRDLLLRLVTAERTRAIVALDELSGLSREPGEVRRLIDQMVDARLLVVQTLEG
                     GKGSTAEIIHDSLIEGWPTLRRWLDENQDDAELVDQLRVAAQQWHHKGYDPGLLWRGD
                     AAVEAGKLRARYKGPLSGIERSFLDAVIAHDAAQVRRRRRAIVGGFAALSLIVVATMV
                     LLVIIQRARELAQDNAHQAQIAQRNAEVAEQDARRQRDEAERNLQERIAAEQRTTAIA
                     TAKRVVDAELTKSKEELIRERDNATQSAEQAVRAQRRAEAANREAQTAQAETARANGA
                     LAAALAKEQARVQQLTDKLGSVPITELKPFSRHRTAAP"
     gene            3536..4510
                     /locus_tag="VF516_27580"
     CDS             3536..4510
                     /locus_tag="VF516_27580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111535.1"
                     /translation="MPIAQPRALWTTDLAQQEDGQRVEVTEDSFEWWYFDTILDDGST
                     CVITFFSKVPFVPGPLSPILLLNISPPQGPYQQQQIFLPPASYSASTTHCAVTMGPSW
                     VTGDLRSYELYAEADGLAAHLVLRDVVPGWRTGPFVPPELAAQQPLAEQVVIPSGVAE
                     GTLMYDGRLHRVTGSCYHDHQWGSARAWTAGVAGAKVTSWHWGRARAGDHAIVFAQVL
                     GAVGGGPSEPIAVLFMLARGTRMTYDDDVTALRVTPLDGGGDQGLTVEWTSNLGNVVL
                     TLPSSKQIASLNDGGYRRFLSPALLHSDHDGENVSSQGQAIWEINVVP"
     gene            complement(4520..6622)
                     /locus_tag="VF516_27585"
     CDS             complement(4520..6622)
                     /locus_tag="VF516_27585"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8111536.1"
                     /translation="MHGAHELLTAITVVLCVAAVTTVVFQRLRQPVVLGYLLAGLIVG
                     PHIPFPLIADRAIIQTLSELGVILLMFALGLEFSLRKLVQVGPTAGLTAIIQSSTMVW
                     LGFVVGRAFGWTPRESIFAGAIIAISSTTIIAKAFDEQGIHGKLRELVVGVLIVEDLI
                     GILLMAVLTTVSSGQGMSAGAVARSSAQLVAFLVGLVAVGMLVVPRAIRAIHRLKRPE
                     TTLVASVGICFAIALLAQEFGYSVALGAFLAGSLVAESGQEKQVEQLILPVRDLFAAI
                     FFVSVGMLIDPAVIAQHWAAVAVFTALVIAGKFVGVSLGAFLTGNGTRTSVEAGMSLA
                     QIGEFSFIIAALGISLNATRDFLYPIAVAVSAITTLTTPWLIRAADPVAAFIDRKLPH
                     PLQTFAALYGTWLEQLRAAPARKTTAARTRRWVKLLVIDAAILSALVIGTAMSLDTIA
                     ESLSTTLGVSERVARGLEVVAAAAIAVPFLIGIVRITHRLGLVLAKIALPAAARDQVD
                     LAAAPRRALVVTLQLAVIVLVGLPMLALIQPFLPGWAAALSLGSVLVVLGFVLWRRAT
                     NLQGHVKAGAEMIVEALVAQARKRPAAPDEEALEQIHHLMPGLGELVPVRLDDRSAAV
                     GKTLAQLDLRGITGASVLAIARGDRGIIGPVAGEVLHAGDVLALAGTHDALAAATELL
                     QRPVPRAGQDGSIAPDRP"
     gene            complement(7091..>8938)
                     /locus_tag="VF516_27590"
     CDS             complement(7091..>8938)
                     /locus_tag="VF516_27590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111537.1"
                     /translation="SWNISAERMFGYTAREMLGQSIAKLLPGGRLDEEGAMLARIAHG
                     EKVAAFETQRRRKDGTMIHVSIRLSPIIERDRVVGASKVVRDITGRKQMEEATRRANA
                     YLASAVDSIQDAFALYDEHDRVVLVNSTFRELFGGGSTGTIVGRTFTDVLDASIRAHV
                     FAGDSAEALRERLIVYHRAPSGTIELRTTSGRIFRLYEQTTPEGGTVSLYVDVTVDVI
                     REDELRTARLAAEAASAAKSEFLSSMSHELRTPLNAILGFAELLQRDRKEPPSTRQSD
                     RLAHIHRGGEHLLRLINDILDLSRIESGRLSMSSERVALSSVLAEVISQLQPQAAQHQ
                     ISLGQAPTPDDDAVVVADRTRLSQILMNFGSNALKYGRAHGHAVYRVSRAKATRVRIA
                     LEDDGIGIPKDKHARIFEPFQRAGQEAGPIEGTGIGLAISKRLAELMGGNIGFTSEPG
                     RGSEFWIELSEPVAAKHEVAKTALQAKRVSTLGREGCSYLLVYIEDNPSNIALMQAVL
                     EDLPRISMLTAPTAEAGIELIRTRHPDLIIMDINLPGMSGIEAIRKLAERADTREIPV
                     IALSAAALPRDTARANDAGFRRYLTKPVNIGELTAAFEEILLAATDRES"
BASE COUNT         1409 a         3165 c         3093 g         1271 t
ORIGIN      
        1 tcgctcggtc gatgggagcc gtgcgccggc gcgagagcgc tgcaagacga tggcgaggcc
       61 gcaagacgtg cggcaactcg ccgagcgccg ggctgagctg gcgccatttc gcgcacgctc
      121 gaccgatggc gcacgggttg cgaagggagg tcgcgagcgg gcgccgcttg cgcagtcatc
      181 accacgtagt ggttctggtc ttgcaatcgc gctcgccaga tcacttctga tcgcatcgtc
      241 accgttaccc gggtcacgga ggttcggtat gagtgacaat gacagaacgc tgttcggcac
      301 gggcgcgagc tgggcatcga gccagcccat ggtgacgctg ccgatcggga tggtgccacg
      361 gccggcccgg gttgccggag ggacgccgta ccccggcccg cggtcccccg ccgcgccgcc
      421 cgccccgctg ccggtgccgg aggcgccggg agggactggc cagtcgccgt gggactcgtc
      481 cggcgacgcg gtcaccgcgg tccacgcgat cagcgcgacg ctgcagccgg gcacgcggat
      541 ccaccagtac gagatcatca agatgatcgg cgaaggcggc atgggcagcg tgttcctcgc
      601 ccgcgatctc cggctcggtc gccgcgtcgc gatcaagttc ctgcgctcgg acgagcccga
      661 gcagacccag cggttcctcg tcgaggcgcg caccacggcg cgctgccagc acgacaacat
      721 cgtcgtgatc tacgaggtcg gcgagcacga cggtgcaccg tacatcgtgc tcgagtacat
      781 gaacggcaag ccgctgagtc acctgaccga ggacggccag aagctgccgt acacccgcgc
      841 ggtggaggtc gtgatcccga tcctccgcgc gctgggctgt gcgcacatgc agggcatcgt
      901 ccaccgtgac ctcaagcctg acaacgtctt cctcaccgag tccggcacga tcaaggtgct
      961 cgacttcggg atcgccaagg tgctgcaggg gcacgccggc ggcgatggcg aggccgcggc
     1021 gacccggatg ccgagcccgc tcgagctcgc gaccggcacc gacgcgggcc tgacccgggt
     1081 cggggcgatc atgggcacgc tgcagtacat gtcccccgag cagtggggca tcggcgtcga
     1141 gatcgaccac ctcaccgata tctgggcgtg cggtgtcctg ctccaccgcc tgatctgcgg
     1201 ccggcacccg ctgtacccgc tcgacggcaa ccagctggtc gtgaccgcga tgctcgacgt
     1261 cgcgatgccg tcgatggtcg cggcgacgcc gcccgacgtg ccgcgcgagc tgatccagat
     1321 cgtcgaccgc tgtctgctca aggacaaggc gcagcgctgg cagtcggccg ccgagctgat
     1381 ggccgcgctc gagccgttcc tgcccggccg gcgcacgcag accctgcagg tcgacgagag
     1441 cccgtacgcc gggctgtcgc cgttccagga gagcgacgcc ggcaagttct tcggacgcaa
     1501 ccgggagatc accgcgatgg tggcccggat tcgcgaccgc ccgctgctgg cggtcgtcgg
     1561 cagctccggc gtcggcaagt cgtcgctggt ccgcgcgggc ctggtgccgg cgctcaagcg
     1621 atccggagag ccgtgggagg tcctggtcgc ccgcccgggg cgcgcgccgc tcgacgcgct
     1681 cgccgacgtg atcgccccga tggtcacgac cgccgccaac ctcgcggatg aggtcggcga
     1741 acacaggaag ctggtcgaca cgctgcgcag cgagcccggc cacctcggac acgtcctgcg
     1801 cctgcgcgcc cgccgcgaca accggcgcat cctgctgttc gtcgatcagt tcgaggagct
     1861 ctacacccag accaccgagg ccgcggagcg cgccgcgttc accgcgtgcc tcgccgcggt
     1921 cgccgacgac gcgaccagcc cgctgcgcgt cgtgctgtcg ttccgcagcg acttcctcga
     1981 tcgcgtcgcc gaggacccgg cgttcgcggg cgagctgacc cagggcctct tgttcgtcgg
     2041 cccgcccggc cgcgaggggc tgcgcgaggc gatcacccag cccgccgagc tcgccgggtt
     2101 ccagttcgag ctgccggcga ccgtcgagga catgctcgac cacctcgaga ccacgcccga
     2161 tgcgttgccg ctgctccagt tcaccgccgc caagctgtgg gagctgcgcg acaccgcgcg
     2221 ccggctgctg acccaccggg cctacgccgc gatcggaggc gtcgccggtg cgctcgcgag
     2281 ccatgccgac cggttcgtcg ccgagctcgg cgcgcaccgg gcgccgctgg tccgcgatct
     2341 gctgctccgc ctggtcaccg ccgagcgcac ccgcgcgatc gtcgcgctcg acgagctctc
     2401 cgggctgtcg cgcgagccgg gcgaggtccg gcgcctgatc gatcagatgg tcgatgcccg
     2461 cctgctcgtc gtccagaccc tcgagggcgg caagggctcg accgccgaga tcatccacga
     2521 ctcgctgatc gagggctggc cgaccctgcg gcgctggctc gacgagaacc aggacgacgc
     2581 cgagctggtc gatcagctgc gcgtcgcggc gcagcagtgg caccacaagg gctacgaccc
     2641 gggcctcttg tggcgcggcg acgccgccgt cgaggcaggc aagctccgcg cgcgctacaa
     2701 gggcccgctg tccggcatcg agcgcagctt cctcgacgcg gtgatcgcgc acgacgccgc
     2761 gcaggtccgg cgccgccgtc gcgcgatcgt cggcggattc gcggcgctgt cgctgatcgt
     2821 cgtcgcgacg atggtgctgc tcgtgatcat ccagcgggcg cgcgagctgg ctcaggacaa
     2881 cgcacaccag gcccagatcg cgcagcgcaa cgccgaggtc gccgagcagg atgccaggcg
     2941 tcagcgcgac gaggccgagc gcaatctcca ggagcggatc gccgcggagc agcgcaccac
     3001 cgcgatcgcc accgccaagc gcgtggtcga tgccgagctg acgaagtcca aggaggagct
     3061 gatccgcgag cgcgacaacg ccacccagag cgccgagcag gcggtgcgcg cgcagcgccg
     3121 cgccgaggcc gccaaccggg aggcgcagac cgcgcaggcc gagaccgccc gggccaacgg
     3181 tgcgctcgcc gccgcgctcg cgaaggagca ggcccgcgtc cagcagctga ccgacaagct
     3241 cggcagcgtg ccgatcaccg agctcaagcc gttctccagg caccggaccg ccgcgccatg
     3301 atccggcgtt cgtcctcgat ccgcgaacgc tcggcaccgg gcactctgtt tcacattcgc
     3361 tgataagacc ttgcctgctt gtgccgcatt cgccggcgcg gtgatcgcga gcgtgcgccc
     3421 gcgctcgcgg aggaatccgg cgacatgtcc tgcacggtac tcgagctcga gagtgtttcg
     3481 gtgaagacgc ggttaggggg acagcgggat ccatcccatc gatcaccctt cgtgaatgcc
     3541 gatcgcacaa cctcgcgctc tgtggaccac ggacctcgcg caacaggagg acggtcagcg
     3601 cgtcgaggtc actgaggaca gcttcgagtg gtggtacttc gacacgatcc tggacgacgg
     3661 ctcgacctgc gtgatcacgt tcttcagcaa ggtgcctttc gtccccgggc cactgtcacc
     3721 gatcttgctg ctcaacatca gcccgccgca ggggccctat cagcagcagc agatcttcct
     3781 gccgcccgcg tcgtacagcg cctcgaccac gcactgcgcg gtgacgatgg gcccgagctg
     3841 ggtcacgggc gatctgcgca gctacgagct gtacgcggag gccgatgggc tcgcggccca
     3901 tctcgtgctg cgcgacgtcg tgccgggatg gcgcaccggt ccgttcgttc cgcccgagct
     3961 ggcggcgcag cagccgctcg ccgagcaggt cgtgattccg tcgggggtcg ccgagggcac
     4021 gctgatgtac gacggccggc tgcatcgcgt gaccggcagc tgctaccacg atcaccagtg
     4081 ggggagcgcc cgcgcctgga cggccggcgt cgcgggagcc aaggtcacct cgtggcactg
     4141 gggacgcgcg cgcgccggag atcacgcgat cgtgttcgcg caggtgctcg gcgcggtcgg
     4201 cggcgggccg agcgagccga tcgcggtcct gttcatgctg gcgcgcggga cgcggatgac
     4261 gtacgacgac gacgtgacgg cgctccgcgt gacgccgctc gacggcggcg gtgatcaggg
     4321 gctgacggtc gagtggacat cgaaccttgg aaacgtggtt ctcacgctgc cgtcgtcgaa
     4381 acagattgcc agtctcaatg acggcggata tcgccgcttc ctgtcgccgg cgctcctgca
     4441 ctccgaccac gacggcgaga acgtctcgag tcagggccag gcgatctggg agatcaacgt
     4501 cgtgccttga acgctcggct cacggccgat ccggcgcgat gctgccgtcc tgcccggccc
     4561 tggggaccgg gcgctggagc agctccgtcg ccgcggcgag cgcgtcgtgc gtgcccgcga
     4621 gcgccaggac atcgccggcg tgcaggactt caccggcgac cggaccgatg atcccccggt
     4681 ccccgcgcgc gatcgcgagc accgaggcgc cggtgatgcc gcgcaggtcg agctgcgcga
     4741 gcgtcttgcc caccgccgcg ctgcggtcgt caaggcgaac cggcaccagc tcgcccagcc
     4801 cgggcatgag gtggtggatc tgctcgagcg cctcctcgtc gggcgcggcc gggcgcttcc
     4861 tggcctgggc cacgagcgcc tcgacgatca tctccgcgcc ggccttgacg tgtccctgca
     4921 ggttcgtcgc gcggcgccac agcacgaagc cgaggacgac cagcacgctc ccgaggctca
     4981 gcgcggcggc ccagccgggg aggaacggct ggatcagcgc cagcatcgga aggccgacca
     5041 ggacgatcac cgcgagctgc agcgtcacga ccagcgcccg ccgcggggcc gccgcgagat
     5101 cgacctggtc ccgggccgcc gcgggcaggg cgatcttcgc gagcacgagc ccgaggcggt
     5161 gcgtgatccg gacgatgccg atcaagaacg gcaccgcgat cgcggcggcg gcgaccacct
     5221 ccagcccgcg cgcgacgcgc tccgacacgc ccagcgtcgt ggacagcgac tccgcgatcg
     5281 tgtcgaggga catcgcggtt ccgatgacga gcgcgctcag gatggcggcg tcgatcacca
     5341 gcagcttcac ccagcgccgg gtccgcgccg cggtggtctt gcgggcgggc gccgcccgga
     5401 gctgctcgag ccacgtgccg tacagcgccg cgaacgtctg cagcgggtga ggcagcttgc
     5461 gatcgatgaa cgccgcgacc gggtcggcgg cgcggatcaa ccacggcgtc gtgagcgtcg
     5521 tgatcgccga gaccgccacc gcgatcggat agagaaagtc gcgcgtggcg ttcagcgaga
     5581 tgccgagcgc ggcgatgatg aacgagaact caccgatctg ggcgaggctc atcccggcct
     5641 cgaccgaggt gcgcgtcccg ttgccggtca ggaacgcgcc gagtgacacg ccgacgaact
     5701 ttccggcgat caccagcgcc gtgaacaccg ccaccgccgc ccagtgctgc gcgatcaccg
     5761 ccggatcgat cagcatgccg accgacacga agaagatcgc cgcaaacaga tcgcgcaccg
     5821 ggaggatcag ctgctcgacc tgcttctcct ggcccgactc ggcgaccagc gagccggcga
     5881 ggaacgcgcc gagcgccacc gagtagccga actcctgggc cagcagcgcg atcgcgaagc
     5941 agatcccgac gctcgcgacc agggtcgtct ccgggcgctt gagccggtgg atcgcccgga
     6001 tcgcgcgcgg cacgaccagc atgccgaccg cgacgaggcc gaccaggaac gccaccagct
     6061 gcgcgctcga ccgcgccacg gctcctgccg acatgccctg tcccgacgag accgtcgtca
     6121 ggacggccat cagcaggatg ccgatcagat cctcgacgat cagcaccccg accacgagct
     6181 cgcgcagctt gccgtggatg ccctgctcgt cgaatgcctt ggcgatgatc gtcgtgctgg
     6241 agatcgcgat gatcgctccg gcgaagatgc tctcgcgcgg cgtccagccg aacgcccgcc
     6301 cgaccacgaa cccgagccac accatggtgc tggactggat gatcgccgtc agccccgcgg
     6361 tcggcccgac ctgcacgagc ttgcgcaggc tgaactcgag gcccagcgcg aacatcagga
     6421 ggatgacgcc gagctcggac agcgtctgga tgatcgcccg gtcggcgatc agcgggaacg
     6481 ggatgtgagg cccgacgatc agcccggcga gcagatagcc gagcacgacc ggctgccgga
     6541 ggcgctggaa cacgacggtc gtcaccgcgg cgacgcagag cacgacggtg atcgccgtca
     6601 gcagttcgtg cgctccgtgc atcctctcca ggtagcatgc aggcccccgg ctcgcgacga
     6661 ccacgagcgc ctcggctcac accgttccgg atcggctcct ggcaggcaca gccccgccgc
     6721 ctcctcgagg ggccgtgggg ccgcacgagg cgcccggttg acgaggggaa ggccgtggct
     6781 agggtgcagg catgctcacg aagaccaacc tcctgctcgc ggcgctcgcg atcacatccg
     6841 gagtcggtgc gagctgcagt gatacgtccg ccggctgccg gtgcggggag cgccggggcg
     6901 accaaggcga ataccgccga caccctcgag agcaaggcga acaacgcggc gaacaaccac
     6961 gaagcgaaca accacgaagc gaacaaccac gaagcggaca accacgaagc ggacaaggcg
     7021 gtgaatcgca aggcggatat cgcgcaccgg gtcactgaga ccgatcagga cgacctctcg
     7081 ggggatcggg tcagctctcc cggtcggtcg ctgcgagcag gatctcctcg aacgccgcgg
     7141 tcagctcgcc gatgttcacg ggcttggtca ggtaacgtcg aaatcctgca tcgttggccc
     7201 gagcggtatc gcgcggcagc gcggccgccg acagcgcgat cacggggatc tctcgcgtgt
     7261 cggcacgctc ggcgagcttg cggatcgctt cgatcccgct catgcccggc aggttgatgt
     7321 ccatgatgat gagatcggga tggcgcgtcc ggatcaactc gatgccggcc tcggccgtgg
     7381 gtgccgtgag catggagatc cgcggcaggt cctcgagcac ggcctgcatc agggcgatgt
     7441 tggacgggtt gtcttcgatg tagacgagca gataggaaca gccctcgcgt ccgagcgtgg
     7501 acacgcgctt cgcctggagc gcagtcttcg ccacctcgtg cttcgcggcc acgggttctg
     7561 acagctcgat ccagaactcg gagccgcgtc ccggctcgct ggtgaacccg atgttaccgc
     7621 ccatgagctc ggcgagccgc ttgctgatcg cgagcccgat gccggtacct tcgatcggcc
     7681 cggcctcctg gccggcgcgc tggaacggct cgaagatcct ggcgtgcttg tctttcggaa
     7741 tgccgatgcc atcgtcctcg agcgcgatcc ggacccgcgt cgccttcgcg cgcgacacgc
     7801 gatagaccgc gtgaccatgg gctcgtccgt acttgagcgc gttggagccg aagttcatga
     7861 ggatctgcga cagtcgcgtc cggtcggcga ccacgaccgc atcgtcgtcg ggcgtcggcg
     7921 cctggcccag ggagatctga tgctgcgcag cctgaggctg gagctgcgag atgacctcgg
     7981 caaggacgct cgacagcgcg acgcgctccg acgacatgct gaggcgcccg gactcgatcc
     8041 gagacagatc caggatgtcg ttgatcagcc ggagcagatg ctcgccgccg cggtggatgt
     8101 gggcgagccg gtcgctctgc cgggtgctcg gtggctcctt gcgatcgcgc tgcaggagct
     8161 ccgcgaagcc caggatcgcg ttgagcggcg tccgcagctc gtggctcatc gacgacaaga
     8221 actcgctctt cgccgcactc gctgcttcgg ccgcgagccg cgcggtgcga agctcgtcct
     8281 cgcggatcac gtcgaccgtc acgtccacgt agagcgagac cgtgccgccc tccggcgtcg
     8341 tctgttcgta gagccgaaag atccgacccg acgtcgttct cagctcgatc gtacccgacg
     8401 gcgcgcgatg gtagacgatc agacgctcgc gcagcgcttc cgcggagtcg ccggcgaaca
     8461 cgtgcgcccg gatgctggca tcgaggacgt cggtgaacgt ccggccgacg atcgtgccgg
     8521 tggagccgcc gccgaacagc tcgcgaaacg tgctgttcac gagcaccacc cggtcgtgct
     8581 cgtcgtacaa cgcgaacgcg tcctggatcg agtcgaccgc gctggccagg taggcgttcg
     8641 ctcgtcgggt cgcctcctcc atctgcttgc gtccggtgat gtcgcgcacg accttcgagg
     8701 caccgacgac gcggtcgcgc tcgatgatcg gcgacagccg gatcgacacg tggatcatcg
     8761 tgccgtcctt gcggcggcgc tgggtctcga acgcggcgac tttctcgccg tgcgcgatcc
     8821 gggcgagcat cgccccctcc tcgtcgagcc gaccaccggg caacagcttc gcgatcgact
     8881 ggccgagcat ctccctggcg gtgtagccga acatgcgctc ggcgctgatg ttccacga
//
LOCUS       DASVOR010001454         4994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_73109,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001454 DASVOR010000000
VERSION     DASVOR010001454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4994
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73109"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1782)
                     /locus_tag="VF516_27595"
     CDS             complement(<1..1782)
                     /locus_tag="VF516_27595"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX8111538.1"
                     /translation="MPSIADKVAGARAMPGLFNLYWDERAGKLWLEIGQWQQEFLYQS
                     ALPAGVGSNDIGLDRGQLGPTRVVRFERIGPKVLLVQSNLAYRAVGAPPDEARTVRES
                     FAESALWGFSVAAETGSRALVDATEFFLRDAHDIPATLKRAGQGAFHVDAARSALVLD
                     HTRSFPRNTEVEATLTFAGDEPGTWVRQVTPTPGAITVREHHSFVALPPPGFRPRAYD
                     PRSSFFGITYQDYATPFSEPLVQRWAARHRLEKKDPTAAVSDPVQPIVYYLDRGVPEP
                     IRGALLEGARRWNAAFEAGGYRNAFRVELMPEGADPMDLRYNVIQWVHRATRGWSYGS
                     TVIDPRTGEILKGHVTLGSLRARQDYLIAEALLAPYGKDAAAGPSPAAQQLVLARLRQ
                     LAAHEVGHTLGLMHNYAASTVGRASVMDYPPPVVALRPDGTLDPSAAYATGIGAWDKV
                     SITWGYQQVPPGTDDRAAGERVLLAAFAAGQRYLTDQDARPAGSASSVAHLWDAGGNA
                     VDELGRILQVRAAALARFGEANLRDRAPLATLEDALVPLYMVHRYQVEAATKLIGGVD
                     YTFALRGDGELATRPVAPAEQRRALTAV"
     gene            1981..2949
                     /locus_tag="VF516_27600"
     CDS             1981..2949
                     /locus_tag="VF516_27600"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX8111539.1"
                     /translation="MQLVDVGRNEPCPCGSGRKYKKCCLAARDAATAVDPALVAAVDR
                     AIEDDDWEPLHDRIDAAMALFERGAPLGHVRFPYDLIRSRNPDRAEMAKMCTAGWIRR
                     CEGEIAHVLDRFQLHAEQRDGLRLAVYLLRRFGALSPTVEEVAKLQVAEHIQRVRRFA
                     DAISARGLTMEQVMAGTADLPEWIERARPTVLSFAEWFALRTATDEQLEFLWLSGVAT
                     RVCDACLDRLDGPSIDDARSWLALAATALFLHRSSLGRVLAHMTSPRQVSETERTAYA
                     GIQALQSSEELRGILHRILRETEHRRAFADEALLRAAIQGLQSSYG"
     gene            complement(3459..4106)
                     /locus_tag="VF516_27605"
     CDS             complement(3459..4106)
                     /locus_tag="VF516_27605"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEX8111540.1"
                     /translation="MRTLLLTAAACTLFLPPALAKDPAQGATAVTVYEGWMSPQQQPG
                     DESDAPKALTKATGLQSTEPSVPREQRKSRGWGQLRFTKDLTRAYVDIEVTGVNPGDI
                     VMFHIHCGPPGVLGPIIVDFGELGSLPKLLATGKMTVELSNKNITYVKQMPRGLKPAL
                     PESCPVEIGFPTQSMTISALEYLARKGVLYFNLHTKANTFYGEVRGQIYPTTDPK"
     gene            4282..>4994
                     /locus_tag="VF516_27610"
     CDS             4282..>4994
                     /locus_tag="VF516_27610"
                     /inference="COORDINATES: protein motif:HMM:NF014789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8111541.1"
                     /translation="MLSRLFAARRVPGASWEDLWEPTGTPVERAAVGGARADRAGHAF
                     AIGYTAALEALAGETRAAFCVTEDGGNHPRAIETRLVDGRVTGTKRWATLADRAAHLL
                     VVARDGTDPAGRPRLRVVRVRRDAPGVTLEVSAAAFVPEISHARVTLDGVAGEVLPGD
                     GYADYVKPFRTVEDIHVHAALVGYWLGVARQHGFAHALIERLAMTAAALVALGAEDAR
                     APATHVALGGVLAASAELVA"
BASE COUNT          668 a         1718 c         1892 g          716 t
ORIGIN      
        1 cacggcggtg agcgcgcggc gctgctccgc gggggcgacc ggacgggtcg cgagctcgcc
       61 gtcgccgcgc agcgcgaacg tgtagtcgac gccgccgatc agcttggtcg cggcctcgac
      121 ctggtagcga tgcaccatgt agagcgggac gagggcgtcc tccagcgtgg ccagcggcgc
      181 gcggtcgcgg aggttggcct cgccgaatcg ggccagcgcg gcggcgcgga cctgcaggat
      241 ccggccgagc tcgtcgaccg cgttgccgcc ggcgtcccac aggtgcgcga cgctgctcgc
      301 cgagccggcc ggccgggcgt cctgatcggt caggtagcgc tggccggcgg caaacgccgc
      361 gagcagcacc cgctctccgg cggcgcggtc gtcggtgccg ggcgggacct gctggtagcc
      421 ccaggtgatc gagaccttgt cccacgcgcc gatcccggtc gcgtaggccg ccgacgggtc
      481 gagcgtgccg tcggggcgca gcgcgaccac cggcgggggg tagtccatca ccgaggcgcg
      541 gccgaccgtg ctcgccgcgt agttgtgcat caggccgagg gtgtggccga cctcgtgcgc
      601 cgcgagctgg cgcagccggg ccagcacgag ctgctgcgcc gccggcgacg gcccggccgc
      661 cgcgtccttg ccgtacggcg cgagcagcgc ctcggcaatc aggtagtcct ggcgcgcgcg
      721 cagcgagccg agcgtgacgt ggcccttgag gatctcgccg gtccgcgggt cgatcaccgt
      781 cgagccgtac gaccagccgc gggtcgcgcg gtggacccac tggatcacgt tgtagcgcag
      841 gtccatcgga tcggcgccct ccggcatcag ctcgacgcgg aacgcgttgc ggtagccccc
      901 ggcctcgaac gcggcgttcc acctccgcgc gccctcgagc agcgcgccgc ggatcggctc
      961 gggcaccccg cgatcgaggt agtacacgat cggctggacc ggatcgctga ccgccgccgt
     1021 cgggtccttc ttctccagcc ggtggcgggc cgcccagcgc tgcacgagcg gctcgctgaa
     1081 cggcgtcgcg taatcctgat acgtaatgcc gaagaagctc gatcgcgggt cgtaggcgcg
     1141 cggccggaac ccgggcggcg gcagcgcgac gaacgagtgg tgctcgcgca cggtgatcgc
     1201 gccgggcgtc ggcgtgacct ggcggaccca cgtgccgggc tcgtcgccgg cgaacgtcag
     1261 cgtggcctcg acctcggtgt tgcgcgggaa gctccgggtg tggtcgagca ccagcgcgct
     1321 gcgcgcggcg tcgacgtgaa acgcgccctg gccggcgcgc ttgagcgtcg ccgggatgtc
     1381 gtgcgcgtcg cgcaggaaga actcggtcgc gtcgaccagc gcccggctgc cggtctcggc
     1441 cgcgacgctg aacccccaca gcgccgactc ggcgaacgac tcgcggaccg tgcgcgcctc
     1501 gtcgggcggg gcgccgaccg cgcggtacgc caggttggat tgcacgagca gcaccttggg
     1561 cccgatccgc tcgaaccgga ccacgcgcgt cgggccgagc tggccgcggt cgagcccgat
     1621 gtcgttcgac ccgaccccgg cgggcagcgc gctctggtac aggaactcct gctgccactg
     1681 gccgatctcg agccacagct tgccggcgcg ctcgtcccag tacaggttga acagccccgg
     1741 catcgcccgg gcgccggcca ccttgtccgc gatcgacggc atcgccggcg ctgccggggt
     1801 cgccgttccc ggcggcctcg gggcggcgct cggcgcagga gcggcggcac aggcggcggt
     1861 gagcgcgagc cagacgggaa ccgatcgcga gagcatgcag ggcctccgcc cgcatgcgta
     1921 tcaccatcgt gcggcgggcg gggcgcggcc ggtgtcagag cgggtgggta gtgtgccgcc
     1981 atgcagctgg tcgatgtcgg acgcaacgag ccatgcccgt gtggcagcgg ccggaagtac
     2041 aagaagtgct gcctcgccgc gcgcgacgcc gcgaccgcgg tcgatccggc gctggtggct
     2101 gccgtcgacc gggcgatcga ggacgacgac tgggagccgc tgcacgaccg catcgacgcg
     2161 gcaatggccc tgttcgagcg cggcgccccg ctgggccacg tccgcttccc gtacgacctg
     2221 atccggtcgc gcaatcccga ccgtgcggag atggcgaaga tgtgcacggc cggctggatc
     2281 cgccgctgcg agggagagat cgcgcacgtg ctcgaccgct tccagctcca cgccgagcag
     2341 cgcgacggac tgcgcctcgc ggtctacctg ctgcggcggt tcggtgcgct gtcacccacc
     2401 gtcgaggagg tcgccaagct ccaggtcgcc gagcacatcc agcgcgtgcg gcgcttcgcc
     2461 gatgcgatct cggcgcgcgg gctcaccatg gagcaggtga tggccggcac ggcggatctc
     2521 cccgaatgga tcgagcgcgc tcggccgacc gtcctgtcgt tcgccgagtg gttcgcgctg
     2581 cgcaccgcga ccgatgaaca gctcgagttc ctctggctgt cgggcgtcgc gacgcgcgtg
     2641 tgcgacgcgt gcctcgaccg gctcgatggc ccctccatcg acgacgcgcg atcgtggctc
     2701 gcgctcgcgg cgaccgcgct gttcctccat cggtcgtcgc tcggccgggt gctggcccac
     2761 atgacctcgc cgcgccaggt cagcgagacc gagcggaccg cgtacgcggg gatccaggcg
     2821 ctgcagtcga gcgaggagct tcgcggcatc ttgcaccgga tcctgcggga gaccgagcat
     2881 cggcgagcct tcgccgacga ggcgctgctg cgtgcggcga tccagggcct ccagagctcg
     2941 tacgggtgag tgaccagcga cagctcgcga tcacgcgcgt gcgtcggtca ccgaggaatc
     3001 acggcgtggc ctgctgcacc ttcgcgatca gctcgctcga ccgacgcgcg gacgatctga
     3061 ggtcctgcag cgccgagcac gcggaccctc actaggtcgt gtcgcgccac cttgtcgcac
     3121 aggaaccgct ggtgagccac tgcctgcgcg gcggcaagga tctcgggagc caccggcggc
     3181 ggccggcaca gcgtacagga tggatccaag ccgccacagt cctggcacgc cctgacttcg
     3241 tcggcatctt ggttctccat ccgcaccatg ctaccggcgt cctttccaaa gccaaaggga
     3301 gatgcgccgg acgagcggat cgcgacggct ggacacaagg aggagcgcag gtacctggcg
     3361 gcacatgacg agccttctgt gcaagaggca gcggctcgac gctcagtaaa aggttaaagt
     3421 accagatgtg gatctccgga tcgcagatcg ggattccatc acttcggatc cgtggtgggg
     3481 tagatctggc cgcggacctc gccgtagaag gtgttggcct tggtgtgcag gttgaaatac
     3541 agcacgccct tgcgcgccag gtactcgagc gccgagatcg tcatcgattg cgtgggaaag
     3601 ccgatctcca ccgggcagct ctcgggcagc gccggcttga ggcccctggg catctgcttg
     3661 acgtaggtga tgttcttgtt ggagagctcg acggtcatct tgcccgtggc gagcagcttg
     3721 ggcaggctgc cgagctcgcc gaagtcgacg atgatcgggc cgagcacgcc gggcgggccg
     3781 cagtggatgt ggaacatcac gatgtcgccc gggttgacgc cggtgacctc gatgtcgacg
     3841 taggcgcggg tgaggtcctt ggtgaaccgc agctggcccc agccgcgcga cttgcgctgc
     3901 tcgcgcggca ccgagggctc ggtgctctgc aggccggtgg ccttggtgag cgccttgggg
     3961 gcgtcggact cgtcgcccgg ctgctgctgc gggctcatcc agccctcgta gacggtgacg
     4021 gcggtcgctc cctgcgccgg atccttggcg agcgcggggg gcaagaacag cgtgcaggcg
     4081 gcggcggtga gcaggagggt ccgcatggaa gttgttctat gcatgagccg accacgttag
     4141 ccgatcttgc cgcgcgctcg ctgccgcgta tcgctccatg cggccgaccc gcgggcggcg
     4201 tacgcggtat cccggatgcg ggcgatcgcg atgggacgcg gcgggccgcc gttgatcggt
     4261 tcgccgcggt aggctcgcag gatgctcagc cggctgtttg ctgcacggcg cgtaccgggt
     4321 gcgagctggg aggacctgtg ggagccgacc ggcacgccgg tggagcgggc cgcggtgggc
     4381 ggcgcgcgcg cggaccgcgc cggtcatgcg ttcgcgatcg gctacaccgc ggcgctggag
     4441 gcgctcgccg gggagacccg cgcggcgttc tgcgtgaccg aggacggcgg gaaccacccc
     4501 cgggcgatcg agacccggct ggtcgacggc cgggtcaccg gcacgaagcg gtgggcgacg
     4561 ctcgcggacc gcgcggcgca cctgctggtc gtcgcgcgcg acggaaccga tccagcgggc
     4621 cggccgcggc tgcgggtggt ccgggtgcgc cgcgatgcgc cgggggtgac gctggaggtc
     4681 tcggccgcgg cgttcgtgcc ggagatctcc cacgcgcggg tgacgctcga tggcgtcgcc
     4741 ggcgaggtgc tgcccggcga cggctacgcg gactacgtca agccgttccg cacggtggag
     4801 gacatccacg tccacgcggc gctggtcggg tactggctcg gggtggcgcg gcagcacggg
     4861 ttcgcgcatg cgctgatcga gcggctggcg atgaccgccg cggcgctggt cgcgctgggc
     4921 gccgaggatg cccgggcgcc ggcgacccac gtcgcgctcg gcggtgtgct cgcggcgagc
     4981 gcggagctgg tcgc
//
LOCUS       DASVOR010001455         2667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_131551,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001455 DASVOR010000000
VERSION     DASVOR010001455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131551"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VF516_27615"
     CDS             <1..1476
                     /locus_tag="VF516_27615"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX8111542.1"
                     /translation="ARFVDPLPVPPVARAADSHPSPGRAGRVPLYRMAMREVEVPVHR
                     DLEPTRWWSYGTTVPGPTLEVRAGEPIAIEWKNELPARHFLPIDHTLCGADARLPEVR
                     TVVHVHGARVPPESDGDPERWFAPGGSRLVHYPNRQDAASLWYHDHTMGIERLNQYAG
                     LFGAYLLRDPVEEALGLPGGPHEIPLFLFDRYLDADGQLWYPTSGSPETPWTSEVYGD
                     AILVNGKLTPDLEVERRMYRFRLFNACNARFLFLTLSSGTPFVQIGSDQGLLPRPVPR
                     PMLALAPAERADVVVDFGALGGQSIVVMNQSIQVMRIRVAPGPAVRSAPLPPVLRDVP
                     RTPRASSVKTRTLALEEYREPTHGMLMLLGGKRWRDPVSEKPVLDSVEIWELANCTED
                     THPIHLHLVRFQVLERQPFDVEAYRFDHKLIHSGPPIPPEPHELGWKDTVQAYADHIT
                     RIIVRFEGHAGRYVWHCHVLEHAANEMMRPFEVVKPSAKRT"
     gene            complement(1484..>2667)
                     /locus_tag="VF516_27620"
     CDS             complement(1484..>2667)
                     /locus_tag="VF516_27620"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX8111543.1"
                     /translation="PEPQGAAGPAPEAQPAEHGWAEGDHLTGDWGGARSALADHGVTI
                     DVFYAGEAFTARSAHSVLGHVDAAITLDTHKLGLWDGGTFYALGQNNHGNSGITGAVG
                     SAQPVTNLEAEPYTQLTELFIEQTFLDERLRIRLGKQDANRDFGTPRFGGNFINNNFG
                     MYPTSPLPSYPTTGLGAIVAAQPVAWLTGKAGIYEGNPDVGGFGLTSAFQTGNGYTLV
                     GGAAATHHYGPADRDGGTTSAGAWWQSGDFTEVGAAGAAMPRTFESNAGWFVQNDERI
                     YLHPSDPDDPRGLTVILRVSWARPDRTSVTRYAGGSAAWHGIGPRRNDTVGLGAGTFR
                     VAEPLGGSPGPGDEWFVEAFYKLRLTAFFSLQPDLQWFHHPGGDGADALVAGLRVKVK
                     L"
BASE COUNT          349 a          953 c          973 g          392 t
ORIGIN      
        1 gccaggttcg tcgatccgct gccggtgccg ccggtcgcgc gggcggccga cagccatccg
       61 tcgccggggc gggccgggcg cgtgccgctg taccggatgg cgatgcgcga ggtcgaggtc
      121 ccggtgcatc gcgacctcga gccgacccgg tggtggagct acgggacgac ggtgcccggc
      181 ccgacgctcg aggtccgcgc gggcgagccg atcgcgatcg agtggaagaa cgagctgccg
      241 gcccggcact tcctgccgat cgaccacacg ctgtgcggcg ccgacgccag gctccccgag
      301 gtccgcaccg tcgtccacgt ccacggcgcc cgcgtgccgc ccgagagcga cggcgatccc
      361 gagcgctggt ttgcgcccgg cggctcgcgc ctggtgcact atccgaaccg ccaggacgcc
      421 gcgtcgctct ggtaccacga ccacacgatg gggatcgagc ggctcaacca gtacgcgggg
      481 ctgttcggcg cctatctcct gcgcgatccc gtcgaggagg cgctcgggct gcccggcggc
      541 ccgcacgaga tcccgctgtt cctgttcgat cgctacctcg acgccgacgg ccagctctgg
      601 tacccgacgt cgggatcgcc cgagacaccg tggacgtccg aggtctacgg cgatgcgatc
      661 ctggtcaacg gcaagctcac gcccgacctc gaggtcgagc gccggatgta ccggtttcgc
      721 ctgttcaacg cgtgcaacgc gcggttcttg ttcctcacgc tgtcgagcgg cacgccgttc
      781 gtgcagatcg gcagcgatca gggcttgctg ccccggccgg tgcccaggcc gatgctggcg
      841 ctcgcaccgg ccgagcgcgc cgatgtggtg gtcgatttcg gcgcgctggg cggccagtcg
      901 atcgtggtca tgaaccagtc gatccaggtg atgcggatcc gcgtcgcgcc gggccccgcg
      961 gtgcgcagcg cgccgctacc gccggtgctg cgcgacgtcc cgcgcacgcc gcgcgcttcg
     1021 tcggtcaaga cccgcacgct cgcgctcgag gagtaccgcg agccgacgca cggcatgctg
     1081 atgctgctcg gcggcaagcg ctggcgcgat ccggtgtcgg agaagccggt gctcgactcg
     1141 gtcgagatct gggagctggc caactgcacc gaggacacgc acccgatcca cctccacctg
     1201 gtccggttcc aggtcctcga gcgccagccg ttcgacgtcg aggcgtaccg cttcgatcac
     1261 aagctgatcc actccgggcc gccgatcccg cccgaaccgc acgagctggg ctggaaggac
     1321 acggtccagg cgtacgccga ccacatcacc cggatcatcg tccggttcga gggccacgcc
     1381 gggcgctacg tctggcactg ccacgtgctc gagcacgcgg ccaacgagat gatgcggccg
     1441 ttcgaggtcg tgaagccgtc cgcgaagcgg acctgaggcg ccgctacagc ttgaccttga
     1501 cccgcagccc ggcgaccagc gcgtcggcgc cgtcgccgcc ggggtgatgg aaccactgca
     1561 ggtcgggctg caggctgaag aaggcggtca gccgcagctt gtagaacgcc tcgacgaacc
     1621 actcgtcgcc ggggccgggg ctgccgccga gcggctcggc gacccggaag gtgccggcgc
     1681 cgagcccgac ggtgtcgttg cggcgcgggc cgatgccgtg ccacgccgcc gagccgccgg
     1741 cgtaccgggt gaccgacgtg cgatccggcc gcgcccagct gacccgcagg atcacggtca
     1801 ggccgcgcgg gtcgtcggga tcgctcgggt ggaggtagat ccgctcgtcg ttctggacga
     1861 accagccggc gttgctctcg aacgtgcgcg gcatggcggc gccggctgcc ccgacttccg
     1921 tgaagtcgcc ggactgccac cacgcaccgg cgctggtcgt gccgccgtcg cggtcggccg
     1981 ggccgtagtg gtgggtcgcc gcggcgccgc cgaccagcgt gtagccgttg ccggtctgga
     2041 acgcgctggt caggccgaac ccgccgacgt cggggttgcc ctcgtagatc ccggccttgc
     2101 cggtcagcca cgccaccggc tgcgccgcga cgatcgcgcc caggcccgtg gtcgggtacg
     2161 acggcagcgg cgaggtcggg tacatgccga agttgttgtt gatgaagttg ccgccgaacc
     2221 gcggcgtgcc gaagtcgcgg ttggcgtcct gcttgccgag ccggatccgc agccgctcgt
     2281 cgaggaaggt ctgctcgatg aacagctcgg tgagctgggt gtagggctcg gcctcgaggt
     2341 tggtcaccgg ctgggccgag ccgaccgcgc cggtgatgcc gctgttgccg tggttgttct
     2401 ggcccagcgc gtagaacgtg ccgccgtccc acaggccgag cttgtgggta tcgagcgtga
     2461 tcgccgcgtc gacgtggccg agcaccgagt gggcgctgcg cgcggtgaac gcctcgccgg
     2521 cgtagaacac gtcgatcgtc acgccgtgat cggcgagcgc gctgcgggcg ccgccccagt
     2581 cgccggtcag gtgatcgccc tcggcccagc cgtgctcggc gggctgcgcc tcgggggccg
     2641 ggccggcggc gccctgcggc tcgggct
//
LOCUS       DASVOR010001456         1690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_190483,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001456 DASVOR010000000
VERSION     DASVOR010001456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1690
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190483"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1690)
                     /locus_tag="VF516_27625"
     CDS             complement(<1..>1690)
                     /locus_tag="VF516_27625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111544.1"
                     /translation="PPAPEGHGDAAARALGLVARAAELFSGPPYADGGARLRQARGAM
                     NAALWPATWGYFLDHMLAPVVPHDAIEPARRHFVDWVRGCGPQPVLRIGEQPYGLLPV
                     LPLAAWTAQDEEPAVAKLAVFLDQTLRPIWRASVAAVPRVSDAPAGGAPDAPQDNPLL
                     TVLSMQPTSVSIRGRSVLGMPFVDAAWRFIRDQLSADQQLGPAWRTEQAALARAVLDQ
                     HNLSDWHPNLEQTVFAANYFPIALPLVQGGTASPSQPLAVDWLTVLGTAGWQALRGDG
                     FDLAERPLLYLLLRHSLLIAYLFAAGDMAPDRPWRGGEPVLYGIDEIDDNLAAPRPDM
                     VWDRLTAPSQAGPAKGDLLDAAPQPPLSSVRDGIAQLVGAPVETLERATAETLDLCSH
                     RLDAWISSFAQRRLHALRGSAGAGGVHLGAYGIVEDLQRGDGSRSAGYVHTPSPSHAT
                     AAAILASGFRSHPGGGGVRRPFGIDLSSERVRVALSLLDGVRAGQPLGALLGYRFERS
                     LQTRGLARFIDDFRRISPLAVSGTAPASGAGEAVGVRNVADALEVHRRWTAAGRV"
BASE COUNT          230 a          618 c          646 g          196 t
ORIGIN      
        1 acgcgcccgg ccgcggtcca ccggcgatgc acctcgagcg cgtccgccac gttgcgcacg
       61 ccgacggcct cgccggcgcc gctcgccggc gcggttcccg acaccgccag cggcgagatc
      121 cgccggaagt cgtcgatgaa gcgcgcgagc ccccgggtct gcagcgagcg ttcgaagcgg
      181 tagcccagga gcgcgccgag cggctggccg gcgcgcacgc catcgagcag cgacagcgcc
      241 acccggacgc gctccgacga cagatcgatc ccgaacggtc gcctgacccc tccgccgcct
      301 gggtgcgacc ggaagccgct ggcgaggatc gcggcggcgg tcgcgtgcga gggcgagggc
      361 gtgtgcacgt agccggcgct gcggctgccg tcgcctcgct ggagatcctc gacgataccg
      421 taggccccca ggtgaacgcc gccggcgccg gcgctgccgc ggagggcgtg cagtcggcgc
      481 tgcgcgaacg acgagatcca ggcgtcgagc cggtgcgagc acagatcgag cgtctcggcg
      541 gtggcgcgct ccagggtctc caccggtgca ccgacgagct gggcgatccc gtcgcggacc
      601 gagctcaggg gcggctgcgg tgccgcatcg agcaggtcgc ccttggccgg accggcctgc
      661 gacggggcag tgaggcggtc ccacaccatg tcggggcgcg gcgcagcgag gttgtcatcg
      721 atctcgtcga tgccgtacag cacgggttct ccgccacgcc acggtcggtc gggcgccatg
      781 tcgccggccg cgaacaggta cgcgatcagc agcgaatggc gcaacagcag gtacagcagc
      841 gggcgctccg cgagatcgaa cccgtcgccg cgcagcgctt gccaacccgc cgtcccgagg
      901 accgtgagcc agtccacggc gaggggttgt gacggcgatg cggtgccgcc ctggaccagc
      961 gggagcgcga tcgggaaata gttcgcggcg aacacggtct gttcgaggtt cgggtgccag
     1021 tccgagaggt tgtgctggtc gagcacggcg cgggcgaggg cggcctgctc ggtccgccac
     1081 gccgggccga gctgctggtc cgcgctcagc tggtcgcgga tgaagcgcca ggcggcgtcg
     1141 acgaacggca tgccgagcac gctgcggccg cggatgctga ccgaggtcgg ctgcatcgac
     1201 agcacggtca gcagcgggtt gtcctgcggg gcatccgggg cgccgcccgc cggcgcgtcg
     1261 ctcacccggg gcacggcggc gaccgaggcg cgccagatcg gccgcagcgt ctggtcgagg
     1321 aacacggcca gcttggccac cgccggctcc tcgtcctgcg ccgtccaggc cgcgagcggc
     1381 agcaccggga gcaggccgta cggctgctcg ccgatccgca gcaccggctg cggcccgcac
     1441 ccgcgcaccc agtccacgaa gtggcggcgc gccggctcga tcgcgtcgtg cggaacgacc
     1501 ggggcgagca tgtggtccag gaagtatccc caggtcgccg gccacagcgc ggcgttcatc
     1561 gctccgcgcg cctggcgcag ccgggccccg ccgtcggcgt acggcggtcc ggagaacagc
     1621 tcggccgctc tcgccacgag cccgagcgcc cgcgcggcgg cgtcgccatg gccctccggt
     1681 gcgggcggcg
//
LOCUS       DASVOR010001457         3062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_307320,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001457 DASVOR010000000
VERSION     DASVOR010001457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307320"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(726..2215)
                     /gene="ltrA"
                     /locus_tag="VF516_27630"
                     /pseudo
     CDS             complement(726..2215)
                     /gene="ltrA"
                     /locus_tag="VF516_27630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004179377.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            2604..2915
                     /locus_tag="VF516_27635"
     CDS             2604..2915
                     /locus_tag="VF516_27635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111545.1"
                     /translation="MKKHRSTQVRVSNEPQNSNFEAGTALENLRAEMIDIEALARAAE
                     AAADALPSPANDRQRLLFDRIQSLATKTSQQASASLRFANAQVTALTAQMEARRGAAG
                     H"
BASE COUNT          557 a         1051 c          886 g          568 t
ORIGIN      
        1 cagatcgtcg tcatgtgccg agtggcactg cgcgatcaat gccagtatcg tgagctccga
       61 tgttattcat tccatgacat cgggcattgg cagcgcgtgc gatctgtggc gcaaagacaa
      121 cgatcctggc cacgcgagta tccagtgact cacagcgaga cagacactcc cgtgatcggc
      181 acatgacgag ctcgcgtatc gatgagctgc cttggatctg tgccactgat tcacaccgcg
      241 ttgctaccga gcgacagctt cgagaggcag tggagctctc acgatgtact catcaccaga
      301 tcggagctca cgaaatcggc atacatggcg caagcgacga cccgacatga cccagacagt
      361 ggcagccgag agcgcgcgga ctcgcactcc cagctgcgcg acgcgcgggg ccgcttcgtg
      421 acggagccgc ctgcctcgcg gcgggcgacg cgcccgcgga cagtggcgcc gctcgatggc
      481 cagggccgcg tccggacgct ctcgtcccga gacggtcatc cgtccatctc gctcaccacg
      541 agacggacgt tcttccgctt ttccgcctac gcgatctgtt cgtcccattc cgggacaggc
      601 cgcgccacct cttggcgcag aaccacaagc ggcccgccga cggatggaac ccgccgacga
      661 gccagtcgga gtaggaatcg caatcgctca cgaccccctc cacagatccg gacaggcggc
      721 gttaccgcat ccggctcttg cctcgggtgg tgacgtcgta ccgcgcttca ggccaaggat
      781 gatagatacg gactggtggg agccatcgct ttacgtatcg gttgatccga tccgcagtga
      841 tccggtgggt ctgactgcgt cgcttgaggg cgttcatcca gcaccagccc acctggtgtc
      901 gaaatgcgct cagctgcggc ccgttcccgg gcactccgta gtagcggaag tgtccatcga
      961 cgacgctacg cagatacgca ccctgctctg gaactgggtc gtgcatgcgt ctccgcagct
     1021 cgaagctaac ttcgcgcagc ttcgcttgca cacgtttccg catcgtcttc ctttgcagga
     1081 catgcctgcc gcgtcggctc tgcccactga tgtgggtaaa cccgaggaag ttgaaggtgc
     1141 ctggcttctg tcctcgaccc gcgcgccatc tcgcccagac ccgaggcccg aacgggacga
     1201 tacgtgtttt gtcgggatgg agctccaggc cgaacgttgc gaaccggtcg cgcagctcgg
     1261 tcaggaaccg ctcggcatct tctcggtgct gaaacccaac gatggtgtca tcggcaaacc
     1321 tcaccgcaac cgtgtcgccg cgattgccgt tcgcccatcg ctggaaccac agatcgaaca
     1381 cgtaatgcaa gtacagattg gccagcagtg gactgatgct tccaccttgt gccgttcctg
     1441 tctcgctcgg tgtccacaac ccatcttctt gcacgccagc ggtcagccat ttctggatga
     1501 gccgcacgac gcgccggtcc gcaatccggt gctcgacgaa cctgactaac cattcgtggt
     1561 cgaccacgtc gaaaaatcca cgaatgtcgg cgtcaagcac ccagttcaca cattttcgtc
     1621 ggagcccaac cgcgagcgca tccagtgctt gatgctgact gcgtcccggt cgaaaaccat
     1681 acgaaaatgc cgcgaagtcc tcctcgtaga tcgcgttcaa cacgtcgacg accgctcgct
     1741 gaacgatctt gtcctccagc gtcggtaccc cgatcggccg ctgtcggccg tctggcttcg
     1801 gaatgtgaac acgtctgacc ggcttcgcgt gatatgctcc tcgtctcagc cgaccggcga
     1861 gatctgtcag gttgcccatg agatcctcgc cgtagcgttg ccacgtttcg ccatcgaccc
     1921 ctgcgctcgc atctcgcttg agagcgaagt aggcggcgcg caggcgatcg atgtcgtaga
     1981 cgtggtgaaa gagcgcagtg aacctctgtt tcctgtcctt cctcgcagct cggcgtactc
     2041 gctcgagagc gctgtgcgcg tcgacccggc gctgagtccg gaacgcgttg ccctcaggca
     2101 agttcccctt ggccaggtcc cttccctcca tcacctccgc agccggtcgc ccggccttgt
     2161 tcggcgattt cctcggtact atggacctgt ccgacttccc gcgaccgttc atcatcggcg
     2221 tgcgtccttg gacttcccga tgcgggctgc accttccagt gcagtcagtt cgcgggacct
     2281 cccggctccc gtgcgtggtg cgtccatacg tgtacggggt cttagaccgc gtggagcccc
     2341 tcggcgcctc gcgaaagtgg cgcgagagtg tagccttccg cgtactccta cagcgtcggc
     2401 actccagaac tcccgtgcaa caggccacgg ggcatcgctt tcgcggctcg atacccgacc
     2461 cgcatgtacc cctgtcaacg cttgaccacc gacgttaccg ccggaggccc atgactcggg
     2521 gcctggtggg tcgctacccc ctttccagtt cgactctttc attcgtaaca ccacgccagt
     2581 tttgccggcg cacaggagcc acgatgaaga agcatcgcag cacccaggtc agggtatcca
     2641 acgagccaca gaacagcaac ttcgaggccg gcacggcgct ggagaacctc cgcgcggaga
     2701 tgatcgacat cgaggcgctc gcgcgcgccg cggaggccgc cgcagacgcc ctgccgtcgc
     2761 cagcgaacga tcgccagcgc ctgctgttcg accggatcca gtcgctcgcg accaagacct
     2821 cgcagcaagc cagcgcctcg ctccgcttcg cgaacgcgca ggtcaccgcg ctcacggcgc
     2881 agatggaggc gcgccgaggc gccgccggtc attgatgtcc cagctgggac tgtggagtcc
     2941 cgggcaccgc cagctccaca gctaagagac ttgccaccgc aactcggcgc aacgatggcc
     3001 ccgcccagac cgccgtctcc gaagatccgg aacggaatca aagaaatccg aaagggagca
     3061 tc
//
LOCUS       DASVOR010001458         4119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_73113,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001458 DASVOR010000000
VERSION     DASVOR010001458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4119
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73113"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VF516_27640"
     CDS             complement(<1..749)
                     /locus_tag="VF516_27640"
                     /inference="COORDINATES: protein motif:HMM:NF012606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HEX8111546.1"
                     /translation="MVVAACSGSDGSEADWMADLADTRGVAELSIPGTHDSGALLEPY
                     PGLAQAQRLAIADQLVAGVRYFDIRCRHFDDAFLIYHGAVDQDQTFDDVLATMRKFLD
                     EHPGEVLIVSIKEEAAPDGNTRSFEATFASYVAQARDRWYLGDTLPRLGDVRGRLVLL
                     RRFAASAAPLGLDGSGWADDTTFSLTTAASLRIEDAYIVSDNAAKWTAITGLLAEARS
                     GDPATLFLDYTSGYQTISGLPNIPSVADDINA"
     gene            930..1817
                     /locus_tag="VF516_27645"
     CDS             930..1817
                     /locus_tag="VF516_27645"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8111547.1"
                     /translation="MPRRTWTLVALALACTACAPSGEPAMHVHVPATAPTRDAEDITV
                     SWIGHATLLIGIHGHWLLTDPVFSERLAGVLPRHVEPALAPSELPPLDAILISHAHFD
                     HLDLPSLRRLADAPLLVPSGMPAMLPRDLPQRRVVALDTWQSWTVGDLRITAVPASHG
                     DGRYWVDRWHTRTHTGWVIELGDRTVYFAGDTGYIADQARELRRRFHIDVDLIPVGPA
                     GRAGWVERLRASVHATPDAALDLFRDTGAQWMVPIHFGTFFQPAGMELPLIEAAVARH
                     HLERSVRILAIGETTTFSY"
     gene            complement(2253..3098)
                     /locus_tag="VF516_27650"
     CDS             complement(2253..3098)
                     /locus_tag="VF516_27650"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8111548.1"
                     /translation="MPDPVESFQRDGYAILERAIDRVFCDELIAALAPFEAGRPMGRN
                     SFEGLRSHRVYSLAGKGEPFRRLAEQPDVLAVVDRVLMPNYLLSTEQSIRLHPGEQAQ
                     KWHTDDAFYHTPRPHPRTLAVTVIWAIEDFTADNGATELIPGSQRWGDEHPDDRPSPA
                     IAAVMPAGSALLFDSALWHRGGHNRSAGTRLAISPQYCQPYLRPQESMLLIVPPDAAR
                     ACSDRMRTLLGYSIHPPFLGQIEGMHPLRLVDPSYREHKTEDHIIADRVLTAPRPGDA
                     TSFAR"
     gene            3219..>4119
                     /locus_tag="VF516_27655"
     CDS             3219..>4119
                     /locus_tag="VF516_27655"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8111549.1"
                     /translation="MLPIRRNIQELQASRIRQVTLAGRDIPDLIPLWFGEGDEVTPAF
                     IRDAAAAALTRGETFYGPNRGIPELRDAIQRYLRRIYRAEVGLDRIVVSASAMNALMI
                     AVQCLVGAGDSAVVVGPVWPNAEKCIEAMGAEVRHVDLEPQPDGSWHLDLGRLIDACD
                     ATTRLIVVNSPGNPTGWMASEDELRGILDHARARGIWLLADEVYARIAYGVRHAPSLV
                     TLAGPDDPVVIVNSFSKSWSMTGWRLGWVIAPAELTNAIEKMTEFNISHPTTFAQWGG
                     VAALDHGDDYVDGLVRRYAEAAEL"
BASE COUNT          565 a         1424 c         1518 g          612 t
ORIGIN      
        1 gcgttgatgt cgtcggccac gctcgggatg ttgggcagcc cgctgatcgt ctggtatccg
       61 ctggtgtagt cgaggaacag cgtcgcaggg tcgccgctgc gcgcctcggc cagcagcccg
      121 gtgatcgcgg tccacttcgc ggcgttgtcg ctgacgatgt aggcgtcctc gatgcggagc
      181 gaggcggcgg tggtcagcga gaacgtcgtg tcgtcggccc agcccgatcc gtcgaggccg
      241 agcggcgccg cgctcgccgc gaaccggcgc agcaggacga gccggccgcg gacgtcgccg
      301 agccgcggca gcgtgtcgcc gaggtaccag cggtcgcgcg cctgggcgac gtacgacgcg
      361 aacgtcgcct cgaagctgcg cgtgttgccg tccggcgccg cctcctcctt gatgctgacg
      421 atcagcacct cgccgggatg ctcgtcgagg aacttcctca tcgtcgcgag cacgtcgtcg
      481 aacgtctgat cctgatcgac cgccccgtgg tagatcagga acgcgtcgtc gaagtggcgg
      541 caccggatgt cgaagtagcg gacgccggcg acgagctggt cggcgatcgc gaggcgctgg
      601 gcctgcgcga ggccggggta cggctcgagg agcgctccgg agtcgtgcgt gcccgggatc
      661 gagagctcgg ccacgccgcg cgtgtccgcg aggtccgcca tccagtcggc ctccgagccg
      721 tccgagccgc tgcacgcggc caccaccatc catgtgacaa cacaaccgca tccgcgtgac
      781 attgcggaag gatagcgtct cgcgggcgcc cgccagcacg gtccggccgc cgctcagcgc
      841 ggcagtgcaa ggccaggagt tccagcacgt tccggctgta ggcggcagtg gcatgcggat
      901 gccgggcgcc gtgtcagact cgacgacccg tgcctcgccg gacctggacg ctcgtcgcgc
      961 tggccctcgc atgcaccgcc tgcgcgccgt cgggcgagcc ggcgatgcac gtccacgtgc
     1021 cggccaccgc gcccacgcgc gacgccgagg acatcaccgt gagctggatc ggccacgcca
     1081 cgctgctgat cgggatccac ggccactggc tgctcaccga tccggtgttc agcgagcgac
     1141 tcgccggcgt cctgccgcgc cacgtcgagc ctgcgctcgc gccgtcggag ctgccgccgc
     1201 tcgacgcgat cctgatctcg cacgcgcact tcgatcacct cgatctgccg tcgctgcgcc
     1261 ggctggcgga cgcgccgctg ctcgtgccat ccggcatgcc ggcgatgctg ccccgcgatc
     1321 tgccgcagcg ccgggtcgtc gcgctcgaca cctggcagtc ctggacggtc ggcgatctgc
     1381 ggatcaccgc ggtgccggca tcgcatggcg acggccgcta ctgggtcgat cgctggcaca
     1441 cgcggacgca caccggctgg gtcatcgagc tcggcgatcg cacggtctac ttcgcgggcg
     1501 acaccggcta catcgccgat caggcccgcg agctccggcg gcggttccac atcgacgtcg
     1561 acctgatccc ggttggcccg gccggccgcg cgggctgggt cgaacggctg cgcgccagcg
     1621 tccacgcgac ccccgacgcc gcgctcgacc tgttccgcga caccggcgcg cagtggatgg
     1681 tgccgatcca cttcggcacg ttcttccagc ccgccggcat ggagctcccg ctgatcgagg
     1741 ccgccgtcgc gcgccaccac ctcgagcgct cggtccggat cctggcgatc ggcgagacca
     1801 ccacgttcag ctactgatcg cagagacgcg gccaaccagg cgaactggcc cggggggcga
     1861 gcgcgctcga tagcggcatc agcgcggctt gctcggcaca tcacgcgcag gtaactccac
     1921 gccctcatca cgcgtcgagc ttgccttctc cggagatccg gaacgaaaca aattcctccg
     1981 gaagggagca tccgcgcggg gcgacgcact gtgagtgggc cgtcctggtc ggtcttgtcc
     2041 gcttcggcgg gccagtcaca gggctgttga cgcggtctcc cggttcatgg ccgagctgct
     2101 tgcgccgatg agggtggtcc ctcgtcggct gttcgaccgg atcccgtgcg atgaagactc
     2161 gcatcaggca cgcgcatcgt ttcgcatcgc cactgggcag gtcgtcgcgc tctcggcgca
     2221 gccgaactcg agccagggcg cgtgttggcc ggtcagcggg cgaacgaggt cgcgtcgccg
     2281 ggccgcggcg cggtgagcac gcggtcggcg atgatgtggt cctcggtctt gtgctcgcgg
     2341 tagctggggt cgaccagccg caggggatgc atgccttcga tctggccgag gaacggcggg
     2401 tggatgctgt agccgagcag cgtgcgcatc cggtcgctgc atgcgcgcgc ggcgtcgggc
     2461 gggacgatca gcagcatcga ctcctgcggc cggaggtagg gctggcagta ctgcgggctg
     2521 atcgcgagcc gggtgccggc ggagcggttg tggccgccgc gatgccacag cgcggagtcg
     2581 aacaggagcg ccgacccggc cggcatgacc gcggcgatcg cggggctggg gcggtcgtcg
     2641 gggtgctcgt cgccccagcg ctggctgccc gggatgagct cggtggcgcc gttgtcggcg
     2701 gtgaagtcct cgatcgccca gatcacggtg accgcgagcg tgcgggggtg cggccgcggc
     2761 gtgtggtaga acgcgtcgtc ggtgtgccac ttctgcgcct gctcgccggg gtggagccgg
     2821 atcgactgct cggtggataa caggtagttg ggcatcagca cgcgatcgac caccgcgagc
     2881 acgtcgggct gctcggcgag ccgccggaac ggctcgccct tgccggccag gctgtacacg
     2941 cgatggctgc gcaggccctc gaaggagttg cggcccatcg gccggccggc ctcgaacggc
     3001 gcgagcgcgg cgatcagctc gtcgcagaac acgcgatcga tggcgcgctc gaggatggcg
     3061 tagccgtcgc gctgaaagga ttcgaccgga tcgggcatgg ggcgagcttc gcatgacggg
     3121 gcgggcgggc gaagcgcggc gcggcggcgc tgttcggctc cggaaccggg ccgactgtga
     3181 ctcgccccgc cgcgcaccgg cgtgtacatt gccgcgcgat gctgccgatc cgacgaaaca
     3241 tccaggagct gcaggcgtct cgcatccgcc aggtcaccct ggcggggcgc gacatccccg
     3301 atctgatccc gctgtggttc ggcgagggtg acgaggtgac gccggcgttc atccgcgacg
     3361 cggcggcggc agcgctgacc cgcggcgaga cgttctacgg cccgaaccgc ggcatccccg
     3421 agctgcgcga cgcgatccag cgctacctgc gccggatcta ccgcgccgag gtcgggctcg
     3481 atcgcatcgt ggtcagcgcg tcggcgatga acgcgctgat gatcgcggtg cagtgcctgg
     3541 tcggcgccgg cgacagcgcg gtggtggtcg ggccggtgtg gcccaacgcc gagaagtgca
     3601 tcgaggcgat gggcgccgag gtgcgccacg tcgatctgga gccgcagccc gacgggtcgt
     3661 ggcacctcga tctcggccgg ctgatcgacg cgtgcgacgc gacgacccgg ctgatcgtgg
     3721 tcaactcgcc gggcaacccg accggctgga tggcgagcga ggacgagctg cgcggcatcc
     3781 tcgaccacgc ccgcgcgcgc gggatctggc tgctcgccga cgaggtctac gcgcggatcg
     3841 cctacggggt gcgccatgcg ccgtcgctgg tcacgctggc cggcccggac gacccggtgg
     3901 tgatcgtcaa ctcgttctcg aagagctggt cgatgaccgg ctggcggctg ggctgggtga
     3961 tcgcgccggc cgagctgacg aacgcgatcg agaagatgac cgagttcaac atctcgcacc
     4021 cgacgacgtt cgcgcagtgg ggcggggtcg cggcgctcga tcacggcgac gactacgtcg
     4081 acggcctggt ccggcgctac gccgaggccg ccgagctgg
//
LOCUS       DASVOR010001459         2499 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_73120,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001459 DASVOR010000000
VERSION     DASVOR010001459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73120"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VF516_27660"
     CDS             complement(<1..985)
                     /locus_tag="VF516_27660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8111550.1"
                     /translation="MLGRYQLFARLGSGGHAEIFLAVARGTLGVDKLVVIKRARPEVR
                     AFGAFFVEEARIALRLNHPNLVHSYELGEDGGAHFLAMEHIEGLSLRELFGSPRSRGF
                     APAIWLRVIADALAGLAHVHGLRDYDGTPLGVIHRDISPHNILVSYDGVTKVVDFGTA
                     LARHGRGGEPGVLAGKLAYMAPEQMVGRIDPRSDLFAMGVVLWEMLAGRRLFDGDQGQ
                     VLGEVVHAPIPRLAGVLPDVDPELDALVARAVEKDPSARYQSAHEMRQAIERHLRRSG
                     CAVRELEVGEAMTAAFADVRAELQARIRHAMDGLASGPPAGELPGLTAGTDL"
     gene            1277..>2499
                     /locus_tag="VF516_27665"
     CDS             1277..>2499
                     /locus_tag="VF516_27665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111551.1"
                     /translation="MTIQKALYLAPLVGLSIIALPRAAHADCGAVLAALGDRAVDATC
                     VDSTDLTTANPATTPADNSITGLPPGAFTPTTDRGVISPDAPDRTPITHAVPGLELRG
                     KFADDPTGQARWLIRLPADWNGRLVVAGASGTRSEDNGDFAWSDYVLQKGYAYASQNK
                     GVLNLQIVSLASPTAPDATACRLNPASQIWVRFFDDDPAKPFTQWTQYIAEDARLAQR
                     AALANYHRPPRFTYAVGTSNGGYQVRRAVEEAPELFDGGVDWEGTFIDPRGPNILIDL
                     PPAIASFPAYVASGFDPNSLAARNIRAAGYPPDLVSGTASLWGSYWTQFWEVTQCQWQ
                     KRFDPAFTTYPGGLNDIDGTAGYDYAGRVAADPAIAQRIAQVASTGRIRRPLITVAGT
                     MDALLPIQRGARAYEA"
BASE COUNT          364 a          946 c          872 g          317 t
ORIGIN      
        1 cgaggtcggt gccggcggtc agcccgggca gctcgccggc cggcgggccg ctggccaggc
       61 cgtccatcgc gtgccggatc cgggcctgga gctcggctcg cacgtcggcg aacgccgcgg
      121 tcatcgcctc gccgacctcg agctcgcgca ccgcgcaccc ggaccggcgc aggtggcgct
      181 cgatcgcctg gcgcatctcg tgggccgact ggtagcgcgc cgacgggtcc ttctccaccg
      241 cccgcgcgac cagcgcgtcg agctcggggt cgacgtcggg caggaccccg gcgagccgcg
      301 ggatcggggc gtggacgacc tcgcccagga cctggccctg atcgccgtcg aacagccggc
      361 gcccggccag catctcccac agcacgacgc ccatcgcgaa cagatcgctc cgcggatcga
      421 tccggccgac catctgctcg ggcgccatgt aggccagctt gccggcgagc acgcccggct
      481 ctccgccccg gccgtggcgc gccagcgcgg tgccgaagtc gaccaccttg gtgacgccgt
      541 cgtagctgac caggatgttg tggggcgaga tgtcgcggtg gatcacgccg agcggcgtgc
      601 cgtcgtagtc gcgcaggcca tggacgtggg cgaggccggc gagcgcgtcg gcgatgacgc
      661 gcagccagat cgcgggcgcg aagccgcggc tgcgcgggct gccgaacagc tcgcgcagcg
      721 acaggccctc gatgtgctcc atcgccagga agtgcgcgcc gccatcctcg ccgagctcgt
      781 agctgtgcac gaggttgggg tggttgagcc ggagcgcgat gcgggcttcc tcgacgaaga
      841 acgcgccgaa cgcgcggacc tcgggccggg cgcgcttgat cacgaccagc ttgtcgacgc
      901 ccagcgtgcc gcgcgcgacc gcgaggaaga tctcggcgtg gccgccgctg cccagccggg
      961 caaacagctg gtagcggccg agcatggcgg cgccgacgtc cgcgcgctct ggcgggagca
     1021 gcggggctgt gcctggctgt tcgctgggat cggaggtcgg catggcgctg aaccccccct
     1081 ttggacgtcg cggaaacgta tcgcaaccca cccggcaggc caacgatgca gtggacaaac
     1141 gcagccgtcg cgtcagcgat gacgcatggc gtaccgcggc acgagcgtcg atcatgacgc
     1201 acctcaccgc ggattcctgc ggatctggca acgatcgcac ctggcgcacc ccctgcaacg
     1261 cgccgcggag cccgcaatga ccatccagaa agcgctgtac ctcgccccgc tcgtggggct
     1321 ctcgatcatc gccctgcccc gggccgcgca cgccgactgc ggcgccgtgc tcgccgcgct
     1381 cggcgatcgc gcggtcgacg cgacctgcgt cgacagcacc gatctgacga ccgccaaccc
     1441 cgcgaccacg ccggcggaca actcgatcac cgggctgccg cccggagcgt tcacgccgac
     1501 caccgatcgc ggcgtgatct cgcccgacgc tcccgatcgc acgccgatca cccacgcggt
     1561 gcccggcctc gagctgcgcg gcaagttcgc cgacgatccg accggccagg cacgctggct
     1621 gatccggctg ccggcggact ggaacggccg gctggtggtc gccggcgcat cgggcacccg
     1681 cagcgaggac aacggcgact tcgcgtggag cgactacgtg ctccagaagg gctacgcgta
     1741 tgcgtcgcag aacaagggcg tgctcaacct ccagatcgtc agcctggcat cgccgaccgc
     1801 accggacgcc acggcgtgcc ggctcaaccc ggcgtcgcag atctgggtgc ggttcttcga
     1861 cgacgacccc gccaagccgt tcacccagtg gacgcagtac atcgccgagg acgcccggct
     1921 ggcgcagcgc gccgccctgg ccaactacca ccgcccgccg cgcttcacct acgcggtcgg
     1981 cacgtcgaac ggcggctacc aggtccggcg cgcggtcgag gaggcgccgg agctgttcga
     2041 cggcggggtc gactgggagg gcacgttcat cgacccgcgg ggaccgaaca tcctgatcga
     2101 cctgccgccc gcgatcgcca gcttcccggc ctacgtcgcg tcgggcttcg atccgaacag
     2161 cctcgccgcc cgcaacatcc gggccgccgg ctatcccccc gacctggtca gcggcacggc
     2221 ctcgctgtgg ggctcgtact ggacccagtt ctgggaggtc acccagtgcc agtggcagaa
     2281 gcggttcgac ccggcgttca cgacctaccc cggcgggctc aacgacatcg acggtaccgc
     2341 cggctacgac tacgccggcc gggtcgcggc cgacccggcg atcgcccagc gcatcgccca
     2401 ggtcgcgtcc accgggcgga tccggcggcc gctgatcacc gtggccggca cgatggacgc
     2461 gctgctgccg atccagcgcg gcgcgcgggc ctacgaggc
//
LOCUS       DASVOR010001460         2662 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_160784,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001460 DASVOR010000000
VERSION     DASVOR010001460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160784"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VF516_27670"
     CDS             complement(<1..1369)
                     /locus_tag="VF516_27670"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8111552.1"
                     /translation="MRPRDRISLAIGGAALLVSVLAIGGVLRWTQAVVAALIALALVM
                     QIGSRRRLDHTSPIVVLLGVAIALTAVQLIPLPAGLLDALDPRGMELRHDGAALAGTA
                     PWQVISLDPAGTLRALAFFATLLGVALLGLRVASSERGRYLVLAAVAMTCGLAAAVTG
                     VHTLLNAESLYGVYRPEHAVPLIFGPLLNTNHLGGLMAIGAVLAVGLAFYQRQAVQLR
                     VLWVVIAIGCSATAMASLSRGATLGLALGVATAIALYAAGRIATVNDDGGRPHRALIT
                     EVPITVVIAAGLAVAVYISAGKVADQLQNTSMTELGYPLSKYEAWRSAFQLVGEAPWV
                     GIGRGAIEPVFTRVHEASAYVTFSHLENEYVQAIVEWGIPGAVVLGLALAWCIRTAVR
                     RWRDGPLAAAAIGACAAILFQSSVDFGIELLGLAVPVVLIATTVLGVRLRESRALVPR
                     LARGAV"
     gene            complement(1369..1935)
                     /locus_tag="VF516_27675"
     CDS             complement(1369..1935)
                     /locus_tag="VF516_27675"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX8111553.1"
                     /translation="MVWLCSACHDDPPVVYPTTAPLDVNKLALGPGDTLNLTVFYGSH
                     SFQASYTLDGSGEISVQYIGTVAANGKTVEQVRDDIKHRLADGYLNDPIVSLTIAELN
                     SLTLSVSGMVAKTGAVKFTPGITITEVIAQAGGFTPLARKNMVKVTRLFNGVKATYKL
                     PVELIAEGERPNFPMLPGDEVFVPERAW"
     gene            2081..>2662
                     /locus_tag="VF516_27680"
     CDS             2081..>2662
                     /locus_tag="VF516_27680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111554.1"
                     /translation="MTDRTALGRLSTQLEKAMKLLAKLGLLGAFGLWVGGATVTAVYG
                     DPPGDPGPPISPSGQSGGASAAGTGASASVDLRGTLPAADVRARVQILHDQIRADARH
                     IQHLQQVARQEKDVIKLNCVNDKLVQAKPQMNIADLKEQELESAGDAERMAAFETISQ
                     AADSLRKLREESDQCIGEPITFSGGESSNSFTGP"
BASE COUNT          431 a          946 c          949 g          336 t
ORIGIN      
        1 cgacggcgcc gcgcgccagc cgggggacga gcgcgcgcga ctcgcgcagc cggacgccga
       61 gcaccgtggt cgcgatcagc acgaccggca ccgcgaggcc gagcagctcg atcccgaagt
      121 cgacgctgct ctggaacagg atcgccgcgc acgcgccgat cgccgccgcc gcgagcggtc
      181 cgtcgcgcca ccgccgcacg gcggtccgga tgcaccacgc cagcgcgagg ccgagcacca
      241 ccgcgccggg gatgccccac tcgacgatgg cctggacgta ctcgttctcg aggtgcgaga
      301 acgtgacgta ggcgctcgcc tcgtggaccc gggtgaacac cggctcgatc gcgccgcggc
      361 cgatcccgac ccacggcgcc tcgccgacca gctggaacgc cgaccgccac gcctcgtact
      421 tggagagcgg gtagccgagc tcggtcatcg aggtgttctg gagctgatcg gcgaccttgc
      481 cggcgctgat gtacaccgcg accgcgaggc cggccgcaat cacgaccgtg atcggcacct
      541 cggtgatcag cgcgcggtgg ggccggccgc cgtcgtcgtt caccgtggcg attcgcccgg
      601 cggcgtacag cgcgatcgcg gtcgcgacgc cgagcgccag tccgagcgtc gcgccgcgcg
      661 acagcgacgc catcgcggtc gcgctgcagc cgatcgcgat cacgacccac agcacgcgga
      721 gctgcaccgc ctggcgctgg tagaacgcca ggccgaccgc gagcaccgcg ccgatcgcca
      781 tcagcccgcc gaggtggttg gtgttgagca gcgggccgaa gatcagcggc accgcgtgct
      841 cgggccggta gaccccgtac aggctctcgg cgttgagcag ggtgtgcacg ccggtcaccg
      901 cggcggcgag cccgcaggtc atcgcgaccg cggcgagcac gaggtagcgg ccgcgctccg
      961 agctcgcgac ccgcagcccg agcagcgcga cgccgagcaa ggtggcgaag aacgcgagcg
     1021 cgcgcagcgt gccggcgggg tcgagcgaga tcacctgcca cggcgcggtc ccggcgagcg
     1081 ccgcgccgtc gtggcgcagc tccatgccgc gggggtcgag tgcgtcgagc agcccggccg
     1141 gcagcgggat cagctggacc gcggtcagcg cgatcgcgac gccgagcagc acgacgatcg
     1201 gcgaggtgtg gtcgagccgg cggcgcgagc cgatctgcat caccagcgcg agcgcgatca
     1261 gcgcggcgac caccgcctgg gtccagcgca gcacgccgcc gatcgcgagc accgacacga
     1321 gcagcgccgc gccaccgatc gccagcgaga tccggtcgcg cggccgcatc accaggcgcg
     1381 ctcgggtacg aatacctcgt cgcccggcag catcgggaag ttcggccgct cgccctcggc
     1441 gatcagctcc accggcagct tgtaggtcgc cttcacgccg ttgaacagcc gcgtcacctt
     1501 gaccatgttc ttgcgcgcca gcggcgtgaa cccgccggcc tgtgcgatca cctcggtgat
     1561 cgtgatcccg ggcgtgaact tcaccgcgcc ggtcttggcg accatcccgg acaccgacag
     1621 cgtgagcgag ttcagctcgg cgatcgtcag cgagacgatc gggtcattga ggtagccgtc
     1681 ggcgagccgg tgcttgatgt cgtcgcggac ctgctcgacg gtcttgccgt tggccgcgac
     1741 cgtgccgatg tactggaccg agatctcgcc ggagccgtcg agcgtgtacg acgcctggaa
     1801 cgagtgtgat ccatagaaca cggtgaggtt cagcgtgtcg ccgggcccga gcgcgagctt
     1861 gttgacgtcg agcggcgccg tcgtcgggta gaccaccggc gggtcgtcgt gacaggccga
     1921 acacagccag accaacgtcg tgagaaccaa gaggaattgg cgcatgatcg cggcgcgccg
     1981 cagccagtcg gcgacccgag tatgttgccg tctgcccggg cgaggttcat cgccggccga
     2041 tgaacgcccc ggcgctgccg ggccaccaac gaggcaagcg ttgacagatc gtaccgcgct
     2101 cggtaggctg tccacgcagc tggagaaggc catgaaattg ctggcaaaac ttggattgct
     2161 cggcgctttc gggctctggg taggcggcgc gaccgtgacc gcggtgtacg gcgatccgcc
     2221 gggcgacccg gggccaccga tcagcccgag cggccagtcc ggcggtgcca gcgcggccgg
     2281 gaccggggcc agcgcctcgg tcgatctgcg cggcacactg ccggcggccg atgtccgcgc
     2341 gcgcgtccag atcctgcacg accagatccg cgccgatgcg cgccacatcc agcacctgca
     2401 gcaggtcgcg cgccaggaga aggacgtcat caagctcaac tgcgtcaacg acaagctggt
     2461 gcaggccaag ccgcagatga acatcgccga cctcaaggag caggagctcg agtccgccgg
     2521 tgacgccgag cggatggcgg cgttcgagac gatctcgcag gccgccgaca gcctccgcaa
     2581 gctgcgcgag gagtccgatc agtgcatcgg cgagccgatc acgttcagcg gcggcgagtc
     2641 gtcgaactcg ttcaccgggc cc
//
LOCUS       DASVOR010001461         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_15717,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001461 DASVOR010000000
VERSION     DASVOR010001461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15717"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VF516_27685"
     CDS             complement(<1..360)
                     /locus_tag="VF516_27685"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8111555.1"
                     /translation="MTRPARSLDELIDDLAGPDLNGRSRALALVLAHGRAALPGLLRT
                     LGHADAEVRVLAAGGLAHLADPSCADTFAGMLADPDERIRAQGACGLARLHDPRALDA
                     LIQTLDDDPDPLRHPTSP"
     gene            complement(357..2165)
                     /locus_tag="VF516_27690"
     CDS             complement(357..2165)
                     /locus_tag="VF516_27690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8111556.1"
                     /translation="MRQQKGQGVGSDRRHDTSVQRKATGTPGRSTLVEHVYGDAVQRR
                     AAPGGGAEPAHVHADAERGVAGAGGSLPHLDRIQRLFGPRHDVSGVQAHVGGEAASAC
                     DAIGASAYATGNHVAFRGAPDLHTAAHEAAHAVQQRAGVQLLGGVGVAGDPYEQHADA
                     VADKVVRGESAEALLDQHSGGGGGAAVQCSFTESRPIPGSTSGFEMDMQTRNGATATP
                     ATKSGLDGYIRFVPGAGDQNSNTITIVQIAKLVDARPATAGRDVRGSVVPLDRHPRGH
                     LGDPGLRTEDDPDRGVEGGYATDVYHNSTGAGTALSPRYNFQPAPAGTTGHGLTQQPP
                     QYGGGTGGVMGNTPGFKRSDLPEDIKSATMYDYPGTTSTTLDLDFEFQSVARGEDTQH
                     DYGSVNWGFGLRAGTVVNEHHTVVAGASATFREALDRHRDFYVHEPVTVYFEFDHDNV
                     TAAEEAKITDLAGYLARNAAVVMTLDGFADQIGDAAYNVALSQRRVNNVRASILAHHP
                     AVPAANVVANTVVPGGGHGISTAATDATSQQPAGTGDQGGNAANGADQSREANRQFNR
                     RVTITFSHPPGTGPAAPGGVGNPTPAPAPAGGGGGS"
     gene            complement(2413..2820)
                     /locus_tag="VF516_27695"
     CDS             complement(2413..2820)
                     /locus_tag="VF516_27695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8111557.1"
                     /translation="MITKHSHACVYVTDQDRARAFYTEKLGMEVRDDARMGNFRWLTV
                     APKTQPDACIVLMLVAPGPMLSEDQAKQLRGLLEAGVLGAGVLETDDCRRDYDELKAK
                     GVPFVREPKEMPYGIEALMRDDSGNFYSLTQRR"
     gene            complement(2858..3271)
                     /locus_tag="VF516_27700"
     CDS             complement(2858..3271)
                     /locus_tag="VF516_27700"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEX8111558.1"
                     /translation="MGIASRATLRRLVRARDVLHASVQRGTTLDELAAASGLSRGFLV
                     RSFARTFGAPPHQYLVQLRLEHAKRALARGASVTETCLEVGFESLGSFSASFHRRVGV
                     SPRAWQRQARGLVQGLGVPALYIPACFLARYAGHV"
BASE COUNT          440 a         1163 c         1274 g          505 t
ORIGIN      
        1 cggcgaggtg gggtggcgca gcgggtcggg gtcgtcgtcg agggtctgga tcaaggcgtc
       61 gagcgcgcgc gggtcgtgga gccgcgcaag gccgcaggcg ccctgggcgc ggattctctc
      121 gtcagggtcg gcgagcatgc cggcgaaggt gtcggcgcac gacggatcgg cgaggtgtgc
      181 gaggccgccg gcggcgagga cccgcacctc ggcgtcggcg tggcccaggg tgcgcaacag
      241 accgggcagt gcagcacggc cgtgcgcgag cacgagggcg agcgcccggc tgcgcccgtt
      301 gagatccggt ccggccagat cgtcgatgag ctcgtcgagc gaccgcgccg gacgggtcat
      361 gagccgcccc ctccaccggc cggcgccggt gccggcgtcg ggttgccgac gccgccgggc
      421 gccgccggac cggtgccggg cgggtggctg aaggtgatgg tcacgcggcg gttgaactgg
      481 cggttggcct cgcggctctg atccgcgccg ttggccgcat tgccgccctg gtcgccggtg
      541 ccggcgggct gctggctggt cgcgtcggtg gccgcggtcg agatgccgtg cccaccgccg
      601 ggtaccacgg tgttggccac cacgttcgcg gccgggaccg ccgggtgatg ggccaggatc
      661 gaggcccgca cgttgttgac ccgacgctgc gacagggcaa cgttgtaggc cgcgtcgccg
      721 atctggtcgg cgaagccgtc gagggtcatc accaccgccg cgttgcgagc caggtatccg
      781 gccaggtcgg tgatcttggc ctcctcggcg gcggtcacgt tgtcgtggtc gaactcgaag
      841 tagacggtca ccggctcgtg cacgtagaag tcgcggtgac ggtcgagggc ttcgcgaaac
      901 gtcgccgacg ctcccgccac cacggtgtgg tgctcgttga ccaccgtgcc ggcgcgcaga
      961 ccgaagcccc agttgaccga gccgtagtcg tgctgggtgt cctcgccgcg ggccaccgac
     1021 tggaactcga agtcgagatc cagcgtcgtg ctggtggtgc cggggtagtc gtacatcgtg
     1081 gccgacttga tgtcctcggg gagatcggag cgcttgaagc ccggtgtatt ccccatcaca
     1141 ccgcccgtgc ccccgccgta ctgcggcggc tgctgggtca ggccgtggcc ggtggtgccc
     1201 gccggcgcgg gctggaagtt gtaacgcggg ctgagcgcgg tgccggcgcc cgtcgagttg
     1261 tgatagacgt cggtggcgta gccaccctcg accccgcgat ccggatcgtc ctcggtgcgg
     1321 agcccgggat cgccgaggtg gccgcggggg tggcgatcga gcggcaccac gctgccacgc
     1381 acatccctgc cggcggtcgc cggtcgggcg tcgaccagct tggcgatctg aacgatggtg
     1441 atggtgttgg agttctggtc gccggcgccc gggacgaagc ggatgtagcc gtcgagcccg
     1501 ctcttggtcg ccggcgtggc cgtggcgcca ttgcgggtct gcatatccat ctcgaagccg
     1561 ctggtcgaac ccgggatcgg gcgggactcc gtgaagctgc actggaccgc tgcgccgccg
     1621 ccaccgccgc tgtgctggtc gagcagcgcc tcggccgact caccacgcac caccttgtcg
     1681 gcgacggcgt cggcgtgctg ctcgtacgga tcgcccgcga ccccgacacc ccccagcaac
     1741 tggacgccgg ctcgctgctg caccgcgtgc gccgcctcgt gcgcggcggt gtgcagatcg
     1801 ggcgccccgc gaaacgcgac gtggttgccg gtcgcgtacg cgctggcgcc gatcgcatcg
     1861 cacgcactcg ccgcctcgcc accgacgtgc gcctgcacgc cggacacatc gtggcgcggt
     1921 ccgaacagtc gctggatcct gtcgagatgg ggcagcgacc ctccggcccc ggccaccccg
     1981 cgctcggcat cggcgtgaac gtgcgccggc tcggcgccgc cgcccggagc tgcccgccgt
     2041 tgcaccgcgt cgccgtacac atgctcgacc agcgtggacc tgcctggggt cccggtggcc
     2101 ttgcgctgga ccgacgtatc gtggcgccga tcgctgccga cgccctgccc cttctgttgc
     2161 cgcatgccgc tccctccggg tctggtgcgg accatactac gatcgcctgg cgcgctccct
     2221 tccgcgcgct tccctcacag cgtaccgcgc gagcgccgga ggcgacaacg tgtgccagcc
     2281 gaggcggcaa catatgccag cacgtgcgcc ggcaggacca ccagcggggt tcgggcgctc
     2341 tcggggatgt cccgaggcgc cccatgcgtc gtcctgcaac cacctgcgcg tgacgcgcat
     2401 cgagctcgtc gctcagcggc gctgggtcag gctgtagaag ttgcccgagt cgtcgcgcat
     2461 cagcgcctcg atgccgtacg gcatctcctt gggctcgcgg acgaacggga cgcccttggc
     2521 cttgagctcg tcgtagtcgc ggcggcagtc gtcggtctcg agcacgccgg cgccgagcac
     2581 gccggcctcg agcaggccgc ggagctgctt ggcctggtcc tcgctcagca tcgggccggg
     2641 ggcgaccagc atcaggacga tgcaggcgtc gggctgggtc ttgggggcga cggtgagcca
     2701 gcggaagttg cccatccgcg catcgtcgcg gacctccatg ccgagcttct cggtgtagaa
     2761 cgccctggcg cgatcctggt cggtgacgta cacgcacgcg tgcgagtgct tggtgatcat
     2821 gccgcggtgg tagcgcagat cgccgccgcg gacttcttca aacgtgaccg gcgtagcggg
     2881 ccaggaagca cgcggggatg tacagcgcgg ggacgccgag gccctggacc aggccgcggg
     2941 cctggcgctg ccacgcgcgc ggcgacacgc cgacgcggcg gtggaagctg gcgctgaacg
     3001 agccgagcga ctcgaagccg acctcgaggc aggtctcggt cacgctggcg ccgcgggcga
     3061 gcgcgcgctt ggcgtgctcg aggcggagct ggaccaggta ctggtgcggc ggcgcgccga
     3121 acgtccgggc gaacgagcgg acgaggaagc cgcgcgacag gcccgaggcc gcggcgagct
     3181 cgtcgagcgt ggtgccgcgc tggacgctgg cgtggaggac gtcgcgggcg cgcaccagcc
     3241 tgcgcagcgt ggctcgagag gcgatgccca tcgccaagtc ataccctacc gcccggctcg
     3301 tcggtcggga tccttgtgat gacgcgcgct tcgacacaaa acggtagggc cgggaagaac
     3361 tcgacaccaa gagcggtttg tg
//
LOCUS       DASVOR010001462         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_365614,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001462 DASVOR010000000
VERSION     DASVOR010001462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365614"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VF516_27705"
     CDS             complement(<1..542)
                     /locus_tag="VF516_27705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111559.1"
                     /translation="MSRPLYQAIEQRFAGARGDQIGDVVHADIPVDGAWYRTRLEMQP
                     VASVVVGLPCTDGFELTMRWNDRWINGDGAPRAASFDDSFLVETNDLALAGVWLDHDV
                     RSGLLASRYVPAQLAERDTALLLRDGRWTHQVCNDEVSAVRAEPEPSEHRMADMLAVS
                     LLLATRPTRWAHAFARVAVAL"
     gene            650..>1644
                     /locus_tag="VF516_27710"
     CDS             650..>1644
                     /locus_tag="VF516_27710"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111560.1"
                     /translation="MSRIATEQPRVVWCCSTCGAIYHKDYPRCPADGSEVVIAERDPL
                     LGTQIGHYSIDKLIGEGGMGRVYLAHHAHLPNKRYAVKVLLGDHAASAQMRARFTREA
                     ERAGQLDHPNVVKVVDFGQTGHGLLYIVMDYVDGPSLVSLIADLPMAPARVIPLARAI
                     CKGLAYAHAAGIVHRDLKPENILVSSGPDGPVPRIVDFGLALSIDQGDARLTESGMTM
                     GTPAFAAPEQLSGKPIDHRADLYAFGMTMFEMLTGGMAPFDGHILEVMSARTARDVPR
                     ITERAPHLAIPRELEDIVARLTRRRPADRYAGAHEGIEALDQVRLGPEARKRTEPA"
BASE COUNT          247 a          596 c          581 g          220 t
ORIGIN      
        1 agcgcgaccg cgacgcgcgc gaacgcatgg gcccagcgcg tcggccgggt cgcgagcaag
       61 agcgacaccg ccagcatgtc ggccatccgg tgctcggacg gctcgggctc ggcccggacc
      121 gccgacacct cgtcgttgca cacctggtgg gtccaccggc cgtcgcggag cagcagcgcg
      181 gtgtcgcgct cggcgagctg ggcgggcacg tagcgcgacg cgagcagccc ggaccgcacg
      241 tcgtgatcga gccacacccc ggcgagcgcg aggtcgttgg tctcgaccag gaagctgtcg
      301 tcgaagctcg ccgcgcgcgg cgcgccgtcg ccgttgatcc agcgatcgtt ccaccgcatg
      361 gtgagctcga agccgtcggt gcacggcagg ccgaccacga cgctggcgac cggctgcatc
      421 tcgagccgcg tccggtacca cgccccgtcg accgggatgt cggcgtgcac cacgtcgccg
      481 atctggtccc cgcgcgcgcc ggcgaatcgc tgctcgatcg cctggtacag agggcgagac
      541 acaccagagt gtagcctccg cgacccgcgc gcgctgtacg aactgtgcgc agtgagtgcc
      601 acatgcggta ctttgaacgt tcggcgaacc cgcgtaccct agaccgtgga tgtcccggat
      661 agcgaccgag cagccgcgcg tggtgtggtg ctgctcgacg tgtggagcga tctaccacaa
      721 ggactatccg cgctgcccgg ccgatggctc cgaggtcgtg atcgccgagc gcgatccgct
      781 gctcggcacg cagatcggcc actacagcat cgacaagctg atcggcgagg gcgggatggg
      841 ccgggtgtac ctggcgcacc acgcccacct gccgaacaag cgctacgcgg tcaaggtgct
      901 gctgggcgat cacgcggcga gcgcccagat gcgcgcgcgg ttcacccgcg aggccgagcg
      961 cgccggccag ctcgaccacc ccaacgtcgt caaggtcgtc gacttcgggc agaccggcca
     1021 cggcctgctc tacatcgtga tggactacgt cgacggtccg tcgctggtgt cgctgatcgc
     1081 cgacctgccg atggcgccgg cgcgcgtcat cccgctggcg cgtgcgatct gcaaggggct
     1141 ggcctacgcg cacgccgccg ggatcgtcca ccgcgatctc aagcccgaga acatcctcgt
     1201 gtcctcgggg cccgacgggc cggtgccgcg gatcgtcgac ttcggcctcg cgctgtcgat
     1261 cgatcagggc gatgcccggc tgaccgagag cggcatgacc atggggacgc cggcgttcgc
     1321 ggcgcccgag cagctgtccg gaaagccgat cgaccaccgc gccgacctct acgcgttcgg
     1381 catgacgatg ttcgagatgc tgaccggcgg catggcgccg ttcgacggcc acatcctcga
     1441 ggtgatgtcg gcgcggaccg cgcgcgacgt cccgcggatc accgagcgcg cgccgcacct
     1501 ggcgatcccg cgcgagctcg aggacatcgt cgcgcggctg acccggcgcc gaccggcgga
     1561 ccgctacgcc ggcgcgcacg aggggatcga ggcgctcgac caggtccggc tcggccccga
     1621 ggcgcgcaag cgcaccgagc cggc
//
LOCUS       DASVOR010001463         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_541014,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001463 DASVOR010000000
VERSION     DASVOR010001463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541014"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..789)
                     /locus_tag="VF516_27715"
     CDS             complement(304..789)
                     /locus_tag="VF516_27715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111561.1"
                     /translation="MDAATLDAPPPPDASPDTCPQHIADPMPPLWFDRRDFDLVSDPA
                     GSKALVWKSNGSNPTASFIVRFQNGDHFTGLSFSAYGDGNSGLRNIQVIYQPSISSTP
                     QILAMRDDLGRAAEWGQVTFPGFQQVVLSSNGGVSVQFDVVGTGYYINQVTPVLDRPC
                     P"
     gene            complement(1109..1273)
                     /locus_tag="VF516_27720"
     CDS             complement(1109..1273)
                     /locus_tag="VF516_27720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111562.1"
                     /translation="MIGVAEIFERVAAERADERLARTLHRVLRRQSHIEHLVEPRSAR
                     HERRSASASS"
     gene            1458..1793
                     /locus_tag="VF516_27725"
     CDS             1458..1793
                     /locus_tag="VF516_27725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111563.1"
                     /translation="MLIRAAAVTVTALTITACGVATDPYEHEPSTDEIDQALMCELHM
                     YRCYPGRAGADETCSLACGRPGHCQDYTPGEYIWCELHPSSCRFGFRCCDPWGNPAWD
                     MYCVPGLEP"
BASE COUNT          340 a          738 c          755 g          342 t
ORIGIN      
        1 gagatcgtga cgtgaggccg aagaccgcct cagccgccgg ccacggccac cgacgggccg
       61 gttccggttc cgcaggcttt ggcacctgaa tcggtggcca gcaggcgacg acagaggctc
      121 gtcgcctggt ttcgtgggtg acgcaaacgt gcagatcgcg ttgtccgggg cctcgctcgc
      181 gatggtcggc ggtcgctgtc cggccagaga tgaactgtgt ccggacgtcg gacagtgaga
      241 gagtgagccg ctgatagacg catcgcttcg ctggcccgag tgacggaggt agcgacacgg
      301 tgctcacggg cagggccgat cgaggacggg cgtgacctgg ttgatgtagt agcccgtccc
      361 gacgacgtcg aactggaccg agacgccgcc gttgctggac aagacgacct gctggaagcc
      421 ggggaatgtc acctgacccc attcggccgc tcgcccgagg tcatcgcgca tcgccaggat
      481 ctgcggcgtc gacgagatgc tgggctggta gatcacctgg atgttccgca gcccggagtt
      541 cccgtcgccg taggcgctga acgacagccc ggtgaagtgg tcgccgttct ggaaccgcac
      601 gatgaacgac gcggtggggt tgctgccgtt ggacttccag accagcgcct tgctccccgc
      661 cggatcggag accaggtcga agtccctgcg atcgaaccac agcggcggca tcgggtcggc
      721 gatgtgctga ggacacgtgt ccgggctggc atcaggcgga ggcggagcgt cgagcgtggc
      781 cgcgtccatc agagccacgt ccggcagcgc tgagtcaggc ggcgccgggt gcgcgtcaaa
      841 ttcgaccttg ccacatgcgg cggcagtaag aagcaagagc gtcattcgca tggagacctc
      901 aaaaaggaac gaccgacagc ccgccccacg ttctcgtcct ctctcgcaga gaggttgcgg
      961 cactggcggt cgcgctcgga gtatttccga tggctcgacc tccgcacgcc gaactcacgt
     1021 gactcacgcg ccgctgagcg cctactggcc ggcggagcgc caggctgcga caccggcgcg
     1081 cagtatcacc tgcaccgcgc ctgctcgatc agctgctcgc cgacgccgat cgccgctcgt
     1141 gccgcgcgct tcgcggctcc acgaggtgct cgatgtggct ttggcgccgc agtacgcgat
     1201 ggagggttcg cgcgaggcgt tcatccgccc gttccgccgc tacccgctcg aagatctccg
     1261 cgacgccgat caccggtttc gcgcgtacgc atgccgttgc caggtccggg tctacgttcg
     1321 ctgctcgctg cgatcgtccg cgaggtccac gaagcgggcg tccagcgagc tgaggcaagg
     1381 aagcacgtga ccgggcatca cgatcggggc gcccaacgcg cggcgcgcgc gaccgtgcgc
     1441 ggcggtacct caggcggatg ctgatccgcg ccgccgccgt caccgtcacc gccctgacca
     1501 tcaccgcctg tggtgttgcg accgacccgt atgagcacga gccgtccacc gacgagatcg
     1561 accaggccct gatgtgcgag ctgcacatgt accgctgcta ccccggacgc gccggcgccg
     1621 acgagacatg ctcgctcgcg tgcggccggc ccggtcactg ccaggactac acgccaggcg
     1681 aatacatctg gtgcgagctg catcccagct cgtgccggtt cggcttccga tgctgcgacc
     1741 catggggaaa cccagcctgg gacatgtact gcgttcccgg gctcgaaccg tgatgaggac
     1801 cgcctgcgcg gtacttgtgg ccggcgcgct ggcgtccctt ctggcccgcg cgccgacgca
     1861 gggtgccgcg cccgcaccgt cgacgatacg atgccgcgat ggcgcggtcc tggcgccgac
     1921 cgtgcgcagg aggatgctcc ggtcacgcgc agccctcacc cgagccggcg gcgggcgcgc
     1981 ggatggctcc ggtctcgtta tgcgcgctgg atcggccgcc gcgagatgcc gatggagatc
     2041 gtgacgtgag gccgaagacc gcctcagccg ccggccacgg ccaccgacgg gccggttccg
     2101 gttccgcagg ctttggcacc tgaatcggtg gccagcaggc gacgacagag gctcgtcgcc
     2161 tggtttcgtg ggtga
//
LOCUS       DASVOR010001464         5768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_511681,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001464 DASVOR010000000
VERSION     DASVOR010001464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5768
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511681"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VF516_27730"
     CDS             complement(<1..321)
                     /locus_tag="VF516_27730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111564.1"
                     /translation="MNELEQLGERIAEQAAHLDAAMHRLLADPAEVLAPGSEREATAA
                     DSASHAELCRIDDSAESPQQQRVAESTPAEAPASLDVCPVLDPCSIDDSAESPPQLEV
                     AGSTP"
     gene            712..1023
                     /locus_tag="VF516_27735"
     CDS             712..1023
                     /locus_tag="VF516_27735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111565.1"
                     /translation="MPSHRSTQPRVSKDAENDNFDPGTALENLRAEVIDIEALARAAE
                     AAAEALPMPANDRQRVVFGRILSLATKTSEQASASLRFANAQVTALTAQMEARRKAAD
                     R"
     gene            complement(1628..4369)
                     /locus_tag="VF516_27740"
     CDS             complement(1628..4369)
                     /locus_tag="VF516_27740"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8111566.1"
                     /translation="MTPIRQAVRSLLTVSALVRIAITAAAVAAVVVALSAWIADQTSL
                     HIDWLPTRPIDLLRPSALYLIAIVPVFYLLRTLSLTDLSLAQQLTQATLRSLVIAGIA
                     IALARPSWITEQSKVATIALVDVSDSVSDKQLDAARAYVDQLAAASGDGNLQLITFAE
                     QPIAVRKVDGKPLSAGIARHVGGGAGTDTQAAMQLAYGMYPDGYVPRMVVVSDGNQTQ
                     GDVAVEAYRAKELGVRVSWRTFDQDKTSEIRVAGLTLPEDLKVGQPYEVTAEVWSTEA
                     QKATLALQQDEFPNGLEPVKEVELREGKNLIKFKSDAKHAGATTYHLRLARFEHDTES
                     QNNQAVITAPVKGRPNVLYVEGGILREPGQAGHLQRALQHENIDVTVRGPSGIPSTAK
                     ELEKFDLVLVSDVPARLMGAGQMQALDAYVGQMGGGLIMAGGEDSFGSGGYERTRIEQ
                     LMPVRFDSEKIQEQPDVAVVLVIDRSGSMQGPKLEAAKESARATAEVLSPNDYIAVVA
                     FDSEAQIYVHPTRAQNKMRISNDIARLQSGGGTNIFPGLKDAFEILQGINAKVKHVIL
                     MTDGEAPSDGIAELVQDMRASRITVSCVGVQGADRNLLSMIADAGDGRLYMVEDIGAL
                     PKIFMKETQEAQKSQLVEDVIHVRIAKRVEAIEGTNVENAPPLRGYVTTKPKPTAETI
                     LISDLGEPILARWRYGGGTAVAWTSDVKNRWSTEWIRWNGYPKFWAQVIRSSMRRKVY
                     DSYDLTAKVADGRAHVVVDAIDTGDKFVNELATHLEVIDPATSKTIDKLPMPQTAAGR
                     YTADFRIQKYGSYLLKAVHQRDGKTVAESLGSVALSYPLEYLRTTPDPEPLRHAAQVS
                     GGRDQAQPAEVWDPGQEKVSYTQDLWPYVLLGVLALFLLDLYAKRVRLFGYRTIKFQ"
     gene            complement(4366..>5768)
                     /locus_tag="VF516_27745"
     CDS             complement(4366..>5768)
                     /locus_tag="VF516_27745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111567.1"
                     /translation="PAPAPAPAASAGPNWATKNLAAVDLSGIDVRYLPVGRRSDNVGI
                     IAFNVRRYIANKAAYEVYIEVQNFGQEPAHRQLTLYNGDTAVDVRRIDLAPGQRIHQI
                     YAKLPASADDKLRASLRPVDGPGGSDPFALDDQAYALLPARKKQKVLLVGSPNLYLEG
                     ALLVYENIDLVGRVSAEDYAARPAVADGLDVAVFDDVTPEVVPPPPTSLLYFHPTGPA
                     SPIAVRAEVSTPHMTEVDEGHPVMRWVTLSDVYMDKSDAFAPEAAKGESTLAFSVRDS
                     IIAAKRDGKRKILAIGFSLPAQGRDSATDLPMRVAFPMLLVNALDWFAGDQADLLTTY
                     PTGTRERVPLDGVVGATEAEVRGPDGQLTHTPVIDGLATFYGSRVGYYDLAAKAPDGS
                     VLARIELAANLASPEESDIAPSAKLTLGGPGGKQLAEPEAFAISHSQKLWIYLIVLAA
                     GLILVEWITYHRRITV"
BASE COUNT          846 a         1834 c         2129 g          959 t
ORIGIN      
        1 cggcgtactt cctgccactt cgagctgcgg cggcgattcc gcggaatcgt cgatcgagca
       61 ggggtcgagc accgggcaga cgtccaatga cgctggagcc tcggccggcg tgctctctgc
      121 caccctctgc tgctgcggcg attccgcgga atcgtcgatc cggcacagct cggcatgtga
      181 ggcggagtcg gctgcggtgg cttcgcgctc cgaacccgga gcgagcacct cggcgggatc
      241 ggcaagcagc cgatgcatgg ccgcatcgag gtgcgccgcc tgttcggcaa tccgttcgcc
      301 cagctgttcg agctcgttca tgcgagagtg catatcacat ggctctgaca tttagaccgt
      361 ggtggggtca cacgctcgac ggcctctgtc ggccgccgcc gggtcgcacc gttcgagaag
      421 caatcagcgc ggcaatgctc aatctggatc cgattcatga tctgatatat ttgatacatt
      481 cttgtatgag tgtaaaaaaa cctgatctcg atccgtggat tcatatcccc gacgatgcct
      541 gaattgttcg atatgcagat cagtccgttc ccatatggca gattgacctc tcccactgcc
      601 tttgcgatac acgatcgttc ctttggaacg aggccgcgcc gccgctcggc gcaagcagtc
      661 tgaacatagt ggcccgccgg tgttggagca ccgacgagcc aaggagcgac gatgccttca
      721 catcgcagca cccagcccag ggtatccaaa gacgccgaga acgacaactt cgacccaggc
      781 accgcgctcg aaaacctccg cgccgaggtg atcgacatcg aggcgctcgc ccgcgccgcg
      841 gaggcagccg ccgaggcgct gcccatgccg gcgaatgatc gccagcgcgt ggtgttcggc
      901 cggatcctgt cgctcgccac gaagacctcg gaacaggcga gcgcatcgct ccgctttgcg
      961 aacgcgcagg tcaccgcgct caccgcacag atggaggcgc gccggaaggc cgcggatcgc
     1021 tgagcggtgg gcggctcggc cagcgtgggg cggtcgcggc cgggtgggac gctcgagccc
     1081 ggtcgtgacc cggccggacc tcccattcgt cttcttcttc gaacccaatt taaatccgga
     1141 gcaaggagcg tcggcgcggg gcactgggct gtgagcaggc ggtcaaggtc ggcctggtca
     1201 acttgctccg gctggtcaca ggccaccctc gcgatctccc gaggtctgcg gttcagtcgt
     1261 accctcgggc acggcctggc cggcagtacc caatgcccga agtgccctgc cacccgccgg
     1321 tgattcgctg taacctgcga tttcagcgac gtttagagcc ctcggccggg aatgcacgag
     1381 gcatgtcgtc gagatggctg gagcgtcgcc tcatgcacgc tagtagggcc gttgcaagta
     1441 cttggccatg gcgaggcagc tcgcggccgc acgcgcacgg gcacacgcgg ccggttgcac
     1501 cggcgattga tttggccccg gaccctatcc tagcggctac gcatgcaacg cgattcgcgg
     1561 acggacgacc ggcaggatcg cgccgccagc ctacgccgac gagcgtgggg gaggccagct
     1621 gcctcgtcta ctggaacttg atcgtccggt agccgaacag gcggacgcgc ttggcgtaga
     1681 ggtcgagcag gaacagggcg agcacgccga gcaggacgta gggccagagg tcctgggtgt
     1741 agctgacctt ctcctggccc gggtcccaga cctcggcggg ttgggcctga tcgcggccgc
     1801 ccgagacctg ggcggcgtgg cgcagcggct cgggatccgg cgtcgtgcgc aggtactcga
     1861 gcgggtagga cagggcgacc gagccgagcg actcggcgac ggtcttgccg tcgcgctggt
     1921 ggacggcctt gagcaggtag ctgccgtact tctggatccg gaagtcggcg gtgtagcggc
     1981 cggcggcggt ctggggcatc ggcagcttgt cgatcgtctt gctggtcgcg gggtcgatga
     2041 cctcgaggtg ggtggcgagc tcgttgacga acttgtcgcc ggtgtcgatc gcatcgacca
     2101 cgacgtgggc gcggccatcg gcgaccttgg cggtgaggtc gtagctgtcg tagaccttgc
     2161 ggcgcatcga cgagcggatg acctgggccc agaacttggg atagccgttc caccggatcc
     2221 actcggtcga ccagcggttc ttgacgtcgg aggtccacgc gacggcggtg ccgccgccgt
     2281 agcgccagcg ggcgaggatc ggctcgccga ggtcggagat caggatggtc tcggccgtgg
     2341 gcttgggctt ggtggtgacg tagccgcgca ggggtggggc gttctcgacg ttggtgccct
     2401 cgatcgcctc gacccgcttg gcgatccgga cgtggatgac gtcctcgacc agctggctct
     2461 tctgggcctc ctgggtctcc ttcatgaaga tcttgggcag cgcgccgatg tcctcgacca
     2521 tgtagagccg gccgtcgccg gcgtcggcga tcatcgacaa cagatttcgg tcggcgccct
     2581 ggacgccgac gcacgacacg gtgatccggc tggcgcgcat gtcctggacc agctcggcga
     2641 tgccgtcgga cggggcctcg ccgtcggtca tcaggatgac gtgcttgacc ttggcgttga
     2701 tgccctggag gatctcgaac gcgtccttga ggccggggaa gatgttggtg ccgccgccgg
     2761 actgcaggcg cgcgatgtcg ttggagatcc gcatcttgtt ctgcgcgcgg gtcgggtgga
     2821 cgtagatctg ggcctcgctg tcgaacgcga ccacggcgat gtagtcgttg ggcgacagga
     2881 cctcggcggt ggcgcgcgcg gactccttgg cggcctcgag cttggggccc tgcatcgagc
     2941 ccgagcggtc gatgacgagg acgacggcga cgtcgggctg ctcctggatc ttctcggagt
     3001 cgaaccggac cggcatgagc tgctcgatcc gggtccgctc gtagccgccc gagccgaacg
     3061 agtcctcgcc gccggccatg atcaggccgc cgcccatctg gccgacgtag gcgtcgagcg
     3121 cctgcatctg gccggcgccc atcaggcgcg ccgggacgtc ggagacgagg accaggtcga
     3181 acttctcgag ctccttggcg gtcgacggga tgccgctcgg gccgcgcacg gtgacgtcga
     3241 tgttctcgtg ctgcagggcg cgctggaggt gaccggcctg gccgggctcg cgcaggatgc
     3301 cgccctcgac gtagagcacg ttgggccggc ccttgaccgg cgcggtgatc acggcctggt
     3361 tgttctggct ctcggtgtcg tgctcgaacc tggcgagccg gaggtggtag gtggtggcgc
     3421 cggcgtgctt ggcgtcggac ttgaacttga tcaggttctt gccctcgcgg agctcgacct
     3481 ccttgaccgg ctcgaggccg ttggggaact cgtcctgctg gagcgcgagc gtggccttct
     3541 gggcttcggt ggaccacacc tcggcggtga cctcgtaggg ctggccgacc ttgaggtcct
     3601 cggggagggt caggccggcg acgcggatct cgctggtctt gtcctgatcg aaggtgcgcc
     3661 acgacacccg gacgccgagc tccttggcgc ggtaggcctc gaccgcgacg tcgccctggg
     3721 tctggttgcc gtcggagacg accaccatgc gcgggacgta gccgtcgggg tacatgccgt
     3781 aggcgagctg catcgcggcc tgggtgtcgg tgccggcgcc gccgccgacg tggcgcgcga
     3841 tgccggccga cagcggcttg ccgtcgacct tgcgcacggc gatcggctgc tcggcgaacg
     3901 tgatcagctg gaggttgccg tcgccggacg ccgcggcgag ctggtcgacg taggcgcgcg
     3961 cggcgtcgag ctgcttgtcg gacaccgagt ccgagacgtc gaccagggcg atggtcgcga
     4021 ccttgctctg ctcggtgatc cacgacggcc gggccagcgc gatcgcgatg ccggcgatca
     4081 ccagcgagcg cagcgtcgcc tgggtcagct gctgggcgag cgacaggtcg gtgagcgaca
     4141 gcgtgcgcaa gaggtagaac accggcacga tggcgatcag gtagagcgcc gacggccgca
     4201 gcagatcgat cggccgggtc ggcagccagt cgatgtgcag gctggtctga tcggcgatcc
     4261 acgccgacag cgcgacgacg accgcggcga ccgcggcggc cgtgatcgcg atccggacca
     4321 gcgcggagac ggtgagcagc gagcgaacgg cttggcggat gggcgtcaca cggtgatccg
     4381 tcggtgatag gtgatccact cgacgaggat gagccccgcc gcgagcacga tgaggtagat
     4441 ccacagcttc tggctgtggc tgatcgcgaa cgcctcgggc tcggcgagct gcttgccccc
     4501 cgggccgccg agcgtgagct tggcggacgg cgcgatgtcg gactcctcgg gcgacgccag
     4561 gttggcggcg agctcgatcc gggcgagcac gctgccgtcg ggcgccttgg ccgcgaggtc
     4621 gtagtagccg acgcgcgagc cgtagaacgt cgcgagcccg tcgatcaccg gggtgtgggt
     4681 gagctggccg tcggggccgc ggacctcggc ctcggtggcg ccgaccacgc cgtcgagcgg
     4741 gacgcgctcg cgggtgccgg tcgggtaggt ggtgagcaga tcggcctgat cgccggcgaa
     4801 ccagtcgagc gcgttgacca gcagcatcgg gaacgcgacc cgcatcggca ggtcggtcgc
     4861 gctgtcgcgg ccctgcgccg gcagcgagaa cccgatcgcg aggatcttgc gcttgccgtc
     4921 gcgcttggcg gcgatgatcg agtcgcgcac cgagaacgcc agcgtcgatt cgcccttcgc
     4981 ggcctcgggg gcgaacgcgt cggacttgtc catgtagacg tcggacagcg tcacccagcg
     5041 catcaccgga tggccctcgt cgacctcggt catgtgcggg gtggagacct cggcgcgcac
     5101 cgcgatcggg ctggccgggc cggtcgggtg gaagtacagg agcgaggtcg gcggcggcgg
     5161 cacgacctcg ggggtgacgt cgtcgaacac cgcgacgtcg aggccgtcgg cgaccgcggg
     5221 gcgcgcggcg tagtcctcgg ccgagacccg gccgaccagg tcgatgttct cgtagaccag
     5281 gagcgcgccc tcgaggtaga ggttgggcga gccgaccaag aggaccttct gcttcttgcg
     5341 cgcgggcaac agcgcgtagg cctggtcgtc gagcgcgaac gggtccgagc caccggggcc
     5401 gtcgaccggg cgcagcgacg cgcggagctt gtcgtcggcg ctcgccggca gcttggcgta
     5461 gatctggtgg atgcgctggc cgggcgcgag gtcgatccgg cggacgtcga ccgcggtgtc
     5521 gccgttgtac agcgtgagct ggcggtgcgc cggctcctgg ccgaagttct ggacctcgat
     5581 gtagacctcg tacgccgcct tgttggcgat gtagcgccgg acgttgaacg cgatgatgcc
     5641 gacgttgtcg ctgcgccggc cgaccggcag atagcggacg tcgatgcccg acagatcgac
     5701 cgcggcgagg ttcttggtgg cccagttcgg gccggcggac gcggcgggag cgggggcggg
     5761 ggccggtg
//
LOCUS       DASVOR010001465         1601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_277114,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001465 DASVOR010000000
VERSION     DASVOR010001465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1601
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277114"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1601)
                     /gene="sppA"
                     /locus_tag="VF516_27750"
     CDS             complement(<1..>1601)
                     /gene="sppA"
                     /locus_tag="VF516_27750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEX8111568.1"
                     /translation="GKPGPYEAPDRSPDFDDARPHWGVLGLGGEIVERSALSWSGSRG
                     TELRTLIGRLRELAGDAGLTGVVVRAGGIDISLPDAVELRAAIHDLRRAGKTVACHAE
                     RATNAGYLVLAACDRIAIAPLGDVAITGPAAMPVHIKPLLDKLGIQADFLHVGAYKGA
                     AEPLTLDAPSRQMEETLGAILDRRYQTMVDAIAGERKLAPDAVKALIDTALFPSDQAQ
                     AAGLVDEVAAFEPFRDAVVRGAAWDSLALAPERGNPFAAVQMVMRFLGALPPDRPSGD
                     HVALVYALGTIIDGRGDGVLGAREQIASHTLVAALRAFTADDRVKAVVLRIDSGGGSA
                     QASELIWRAVAELKARKPVIVSMSDVAASGGYYIASGATRIFALDDTLTGSIGVVGGK
                     LAVRGALTRLGVNTFPIGRGKRATMMASLGPWSDDERGAVRSAMEAVYRVFVGRVAAG
                     RGKPPEEIEKIAQGRVWTGARARELGLVDEIGGLDAALAEARKLAKVDAATGLEVYPP
                     APTLRDLVVGLGEVQAPLGLGGQGS"
BASE COUNT          197 a          622 c          581 g          201 t
ORIGIN      
        1 cgctgccctg accgccgagg ccgagcggcg cctggacctc gcccagcccg acgaccaggt
       61 cgcgcagcgt cggcgccggc ggatagacct cgaggccggt ggcggcatcg accttggcca
      121 gcttgcgggc ctcggcgagc gcggcgtcga gcccgccgat ctcgtcgacc aggccgagct
      181 cccgggcgcg cgcaccggtc cacacccggc cctgcgcgat cttctcgatc tcctcgggcg
      241 gcttgccgcg gcccgcggcg acccggccga cgaacacgcg gtacaccgcc tccatcgcgg
      301 accgcaccgc gccgcgctcg tcgtcgctcc acgggccgag gctcgccatc atcgtcgcgc
      361 gcttgccgcg gccgatcggg aacgtgttga ccccgagccg ggtcagcgcg ccgcgcaccg
      421 cgagcttgcc gccgaccacg ccgatcgagc cggtcagcgt gtcgtcgagc gcgaagatcc
      481 gggtcgcgcc gctcgcgatg tagtagccgc cgctggccgc gacgtcgctc atcgacacga
      541 tcaccggctt cctggccttg agctcggcga ccgcgcgcca gatcagctcg gacgcctggg
      601 cgctgccgcc gcccgagtcg atccgcagca ccaccgcctt gacgcggtcg tcggcggtga
      661 acgcgcgcag cgccgcgacc agggtgtgcg acgcgatctg ctcgcgcgcg ccgagcacgc
      721 cgtcgccccg gccgtcgatg atcgtgccga gcgcgtagac cagcgcgacg tgatcgccgc
      781 tcggccggtc gggcggcagc gcaccgagga accgcatcac catctgcacc gcggcgaacg
      841 gattgccgcg ctcgggcgcc agcgccaggc tgtcccacgc ggcgccgcgg accacggcgt
      901 cgcggaacgg ctcgaacgcc gcgacctcgt cgacgagccc ggccgcctgg gcctggtcgg
      961 acgggaacag cgcggtgtcg atcagcgcct tgaccgcgtc gggcgccagc ttgcgctcgc
     1021 cggcgatcgc atcgaccatc gtctggtagc ggcgatcgag gatcgcgccg agcgtctcct
     1081 ccatctgccg ggacggcgcg tcgagcgtca gcggctcggc ggcgcccttg tacgccccga
     1141 cgtggaggaa gtcggcctgg atcccgagct tgtcgagcag cggcttgatg tggaccggca
     1201 tcgcggccgg gccggtgatc gcgacgtcgc cgagcggcgc gatcgcgatc cggtcgcacg
     1261 ccgcgagcac caggtagccg gcgttggtcg cgcgctcggc atggcaggcg acggtcttgc
     1321 cggcgcgccg caggtcgtgg atcgcggcgc gcagctcgac ggcgtcgggc aggctgatgt
     1381 cgatgccgcc ggcgcggacc accacgccgg tcagcccggc gtccccggcg agctcgcgca
     1441 gccggccgat cagcgttcgc agctcggtgc cgcggctgcc ggaccacgac agcgcgctgc
     1501 gctcgacgat ctcgccgccg agcccgagca cgccccagtg cggcctggca tcgtcgaagt
     1561 cgggcgagcg gtcgggcgcc tcgtacggcc ccggcttgcc c
//
LOCUS       DASVOR010001466         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_482689,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001466 DASVOR010000000
VERSION     DASVOR010001466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482689"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..>1703
                     /locus_tag="VF516_27755"
     CDS             258..>1703
                     /locus_tag="VF516_27755"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8111569.1"
                     /translation="MRLRRKITVAFFLVSTLVSAVLAVALYRFVERQLEAELRDKLRD
                     IGHLGVRAIDTAAYRRLVGQLGELDDAAVTAVERSPDWTAIYDQLRQIRAAEPTLIHY
                     AYLLAPTGDPGQPRFVVDADVLELEARVARGEQVEDISRFGKPYDVTDLPLLARALAE
                     CTSQLEPELVLDKAFGVRSMSAYTPLVDARGEPLRDAAGRCLGVLGVDLTDRKMQAAL
                     ASAGHLALEISIAVVALALVVSIVMGTLLTRSVLALSATVRRFAAKDFSARTRVRSRD
                     EIGQLGDSFNAMADIIQVHSDRLEDLVRQRTRELTEEKQTSERLLLNVLPGPIAERLK
                     TGENLIVDRFDAVSVLFADIVGFTALSSRTSPEALVTMLNELFSTFDRLAEQHGLEKI
                     KTIGDAYMVVAGIPHPVPDHAAAITAMALDMLAFVEAYSQRLGLELTIRIGIHTGPVV
                     AGVIGTKKFIYDLWGDTVNTASRMESHGVPGR"
BASE COUNT          240 a          644 c          585 g          234 t
ORIGIN      
        1 cgccgggctc gacggccgcc ggcgccaccg gaaccgccgg aaccgccggt gccgggacct
       61 ccggcctcgg cgtagccggg ccgcaaccgg ccagtgccac gaccacgagc aaccgcattg
      121 ctgcagcatg tcatggcgaa cccaccgacg catcgccggc gcgggccggg cgcgcccgac
      181 gcgcgatcgc cggggcgatg caccccggga gcgccgcatg gtgcggctgc ccccggcctg
      241 ttagcctgag cggcggggtg aggctccggc gcaagatcac ggtcgcgttc ttcctggtca
      301 gcacgctggt cagcgccgtc ctcgcggtgg ccctgtaccg cttcgtcgag cgccagctcg
      361 aggccgagct gcgcgacaag ctgcgcgaca tcggccacct cggcgtccgc gcgatcgaca
      421 ccgccgccta ccgccggctg gtcggccagc tcggcgagct cgacgacgcc gcggtcaccg
      481 cggtcgagcg ctcgcccgac tggacggcga tctacgacca gctccgccag atccgcgccg
      541 ccgagccgac gctgatccac tacgcgtacc tcctggcgcc gaccggcgat cccggccagc
      601 cgcggttcgt ggtcgacgcc gacgtgctcg agctcgaggc ccgggtcgcg cgcggcgagc
      661 aggtcgagga catcagccgg ttcggtaagc cgtacgacgt caccgacctc ccgctgctcg
      721 cccgcgcgct cgccgagtgc accagccagc tcgagcccga gctggtgctc gacaaggcgt
      781 tcggggtccg gtcgatgtcg gcctacaccc cgctggtcga tgcccgcggc gagccgctgc
      841 gcgacgcggc cggtcgctgc ctcggggtgc tcggcgtcga cctcaccgac cgcaagatgc
      901 aggcggcgct cgcctcggcc ggccacctcg cgctcgagat ctcgatcgcc gtcgtcgcgc
      961 tcgcgctggt cgtgtcgatc gtgatgggca cgctgctcac ccgctcggtg ctcgcgctgt
     1021 cggccacggt gcggcggttc gccgccaagg acttctcggc gcgcacccgg gtgcgcagcc
     1081 gcgacgagat cggccagctc ggcgacagct tcaacgcgat ggccgacatc atccaggtcc
     1141 acagcgaccg gctcgaggac ctggtccgcc agcgcacccg cgagctcacc gaggagaagc
     1201 agacctcgga gcggctgctg ctcaacgtcc tgcccggccc gatcgccgag cggctcaaga
     1261 ccggcgagaa cctgatcgtc gaccggttcg acgcggtcag cgtgctgttc gccgacatcg
     1321 tggggttcac cgcgctgtcg tcgcggacct cacccgaggc gctggtcacc atgctcaacg
     1381 agctgttctc gacgttcgac cggctcgccg agcagcacgg gctcgagaag atcaagacga
     1441 tcggcgatgc gtacatggtg gtcgccggca ttccccatcc ggtccccgat cacgcggccg
     1501 cgatcaccgc gatggcgctc gacatgctcg cgttcgtcga ggcctacagc cagcggctcg
     1561 gcctggagct gacgatccgg atcggcatcc acaccggccc ggtggtcgcc ggcgtgatcg
     1621 gcaccaagaa gttcatctac gacctgtggg gcgacacggt gaacaccgcg agccggatgg
     1681 agtcgcacgg cgtgccgggc cgg
//
LOCUS       DASVOR010001467         1929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_44462,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001467 DASVOR010000000
VERSION     DASVOR010001467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1929
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44462"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VF516_27760"
     CDS             complement(<1..492)
                     /locus_tag="VF516_27760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111570.1"
                     /translation="MTTAARTLALAALVIFAGAAAPAAADTIAITGATIYQRSDRKLD
                     SATIVIRDGRIAEVGAGVAVPAGATRIDGKGKIVTAGLIEAATQLGLVEVDLEESAND
                     GRFGTQPTEIHAAYRAVDAYDGRSVAIPVARTGGVTSAITGPIGGLVAGQAAWVSLAD
                     SAAP"
     gene            complement(489..>1929)
                     /locus_tag="VF516_27765"
     CDS             complement(489..>1929)
                     /locus_tag="VF516_27765"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8111571.1"
                     /translation="PTLDIADAWVDPTADTPPRGKRPIRDRAAALHNPGAPAIAIRHA
                     TIMTANGRILERGTIVLTGGAIAALGADAGVTVPAGARVIDGTGKFVTPGLIDAHSHL
                     GVYAAPFAHGNDDGNEATAPVTAQVHAQYGYGPQDPQIARAIAGGVTAALILPGSANL
                     IGGEGFTVVMRPGRTSDEVGFPGAPRTLKMACGENPKRVYGGKAGPSTRMGEYAAFRA
                     AFNEARSYLAKQAAYDRTRALWLKKKARAAELDARQTDPRLKVKVEAAPEVPPVDLRL
                     ETLAGVLRGDVLVQVHCYKASDMAEMVAVADESGFKIRSFHHALEGYKIRDLLAARGI
                     AINTWSDWWGFKLEAFDGIPENTALFTQQGGRATIHSDSAIGIQRLHQDAAKAMAAGR
                     AAGIAISDDQALRWVTANPAWVLGIDDITGTLEVGKRADVAVWSGSPFSVYSRAELVI
                     AGGEVTYDRSQGATPSDFELANSATQRVP"
BASE COUNT          215 a          704 c          722 g          288 t
ORIGIN      
        1 cggggcggcg ctgtcggcga gcgacaccca cgcggcctgg ccggcgacca ggccgccgat
       61 cggcccggtg atcgccgagg tcacgccgcc ggtgcgcgcg accgggatcg cgaccgagcg
      121 gccgtcgtag gcgtcgaccg cgcggtacgc ggcgtggatc tcggtcggct gggtcccgaa
      181 ccggccgtcg ttggccgact cctcgagatc gacctcgacg aggccgagct gggtcgccgc
      241 ctcgatcagc ccggcggtga cgatcttgcc cttgccgtcg atccgggtcg cgcccgccgg
      301 caccgcgacg ccggcgccga cctcggcgat cctgccgtcg cggatcacga tcgtcgcgct
      361 gtcgagcttg cgatccgagc gctggtagat cgtcgcgccg gtgatcgcga tcgtgtcggc
      421 ggcggccggc gccgcggccc cggcgaagat gaccagcgcg gcgagcgcga gcgtcctcgc
      481 tgccgtcgtc atggcaccct ctgggtcgcc gagttggcga gctcgaagtc cgacggcgtc
      541 gcgccctggc tgcggtcgta ggtgacctcg ccgccggcga tcaccagctc ggcgcggctg
      601 tacaccgaga acggcgagcc gctccacacc gcgacatcgg cgcgcttgcc gacctcgagc
      661 gtcccggtga tgtcgtcgat gccgagcacc cacgccgggt tggcggtgac ccagcgcagc
      721 gcctggtcgt cgctgatcgc gatcccggcg gcgcgcccgg ccgccatcgc cttggcggcg
      781 tcctggtgga gccgctggat gccgatcgcg gagtccgagt ggatggtcgc gcggccgccc
      841 tgctgggtga acagcgcggt gttctcgggg atgccgtcga acgcctcgag cttgaacccc
      901 caccagtcgg accacgtgtt gatcgcgatc ccgcgcgccg ccagcaggtc gcggatcttg
      961 tagccctcga gcgcgtggtg gaacgaccgg atcttgaagc cggactcgtc ggcgaccgcg
     1021 accatctcgg ccatgtcgct ggccttgtag cagtggacct ggaccagcac gtcgccgcgc
     1081 agcacgcccg ccagggtctc gagccggagg tcgaccggcg gcacctcggg cgcggcctcg
     1141 accttgacct tgagcctggg atcggtctgc cgggcgtcga gctcggcggc gcgcgccttc
     1201 ttcttgagcc acagcgcgcg ggtccggtcg tacgcggcct gcttggcgag gtagctgcgc
     1261 gcctcgttga acgcggcgcg gaacgcggcg tactcgccca tccgcgtgct cggcccggcc
     1321 ttgccgccgt acacccgctt ggggttctct ccgcacgcca tcttgagcgt gcgcggcgcg
     1381 ccggggaacc cgacctcgtc gctggtccgc cccggccgca tcacgacggt gaagccctcg
     1441 ccgccgatca ggttggccga gccgggcagg atcagcgccg cggtcacgcc gccggcgatc
     1501 gcgcgcgcga tctgcggatc ctgcggtccg tagccgtact gcgcgtggac ctgcgcggtg
     1561 accggcgcgg tcgcctcgtt gccgtcgtcg ttgccgtgcg cgaacggcgc ggcgtacacg
     1621 ccgaggtggg agtgcgcatc gatcagcccc ggggtcacga acttgccggt gccgtcgatc
     1681 acccgcgccc cggcggggac ggtgaccccg gcgtcggcgc cgagcgcggc gatcgctccg
     1741 ccggtcagca cgatcgtgcc gcgctcgagg atccggccgt tcgcggtcat gatcgtcgcg
     1801 tggcggatcg cgatcgcggg cgcgccgggg ttgtgcagcg cggcggcgcg gtcccggatc
     1861 gggcgcttgc cgcgcggcgg tgtgtccgcg gtcgggtcga cccaggcgtc ggcgatgtcg
     1921 agcgtcggc
//
LOCUS       DASVOR010001468         3250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_336117,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001468 DASVOR010000000
VERSION     DASVOR010001468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336117"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(325..1500)
                     /locus_tag="VF516_27770"
     CDS             complement(325..1500)
                     /locus_tag="VF516_27770"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HEX8111572.1"
                     /translation="MKANVWMGKRKVSVEQVPDPRILNARDAIVRVTSTTICGSDLHL
                     YNGFVPTMQRGDILGHEFMGEVVELGSAVKNLQVGDRVVVPFAIACGNCEECQLGNFS
                     VCENANPNAWISEKLMGHSICGAFGYAHLTGGFAGGQAEYVRVPYADFGPLKVPSALK
                     DEQVLFLSDIFPTGYMAADFCEINPRKVIAVWGAGPVGQFAIASARMLGAEKVIAIDR
                     FAYRLDLAVKKAGATDVINYEEVPSVQEALMDLTGGRGPDACIDAVGLEAHGTGAEYA
                     YDKVKQSLRIELDRPIAVRDAIMACRSGGIVSVIGVYAGLVDKFPLGALMNRGLTIKS
                     GQCHVHRYMKPLLERIERGEIDPTFVISHHMKLDDAPRGYDMFVHKRDNAMKIVLRT"
     gene            complement(1497..2468)
                     /locus_tag="VF516_27775"
     CDS             complement(1497..2468)
                     /locus_tag="VF516_27775"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8111573.1"
                     /translation="MNEISVVQSEPPAPDKADATSLIRGLGWLGVTLGVTELVIPRLL
                     ASAIGVDPDGAAPAILRAMGVREIASSSAVLLRPDHPSPRWARVIGDAIDLGLLGLAL
                     TTPGTGKRRLGMALGMVAGVTALDVLAARRQGRARHPLPVIAAVTINKPPMEVYEFCR
                     RLEQLPLFMNHLVSVEQHANRRSTWTARLPVVGTVRWETELVEDRPGQVLSWRSVEGS
                     QVQTRGRVTFTRAPGRDMTEVRIEMQLGVLGREPSIGLARLVARPQVKGDLRRLKQVL
                     ETGEVLRSDASVYRLPHPAQPSPARHEISPPRVFLESPPTAAKGVSP"
     gene            2714..3166
                     /locus_tag="VF516_27780"
     CDS             2714..3166
                     /locus_tag="VF516_27780"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8111574.1"
                     /translation="MKARLEGLTGVFKTLAEQHGEVAAMLKRLQSKPEKKPELWPEIR
                     RELLSHERGEIREVYPVLRQHVQTRALAEHHDQEAAEIEHLIERVDAATADDWRPLFD
                     QLVETVIHHANEEEHNIFPKAQEAIGEQAAKDLEPRFLAAKKQVMATA"
BASE COUNT          519 a         1161 c         1113 g          457 t
ORIGIN      
        1 gtcggctcgg cgccgtcgga caggtaggtc agcgtcaggg tcgtgatcgc gaccgacggc
       61 gcggcgccga acagccccga gaacgacttg ggctcgaaca gctccgcgat cgccgcgaac
      121 gcggagacca cggcgccgcc gagcacgaat cgcagcacca gctcgccgat catgcgagat
      181 ccgccgagcg ctccgtcgcg cagcgaacct gagacccttg cgccatgttc cggcgccgct
      241 gcaagtccgg agcccgctgc gcggcggcgg gccggcacac cggtgcgcat ccgtccgcac
      301 ggtccggacc ggaggacgcc gcgatcaggt gcgcagcacg atcttcatcg cgttgtcccg
      361 cttgtgcacg aacatgtcgt agccgcgcgg ggcgtcgtcg agcttcatgt ggtgcgagat
      421 cacgaacgtc ggatcgatct cgccgcgctc gatgcgctcg agcagcggct tcatgtagcg
      481 gtggacgtgg cactgcccgc tcttgatcgt cagcccgcgg ttcatcagcg cgccgagcgg
      541 gaacttgtcg accaggccgg cgtagacccc gatgaccgac acgatgccgc cgctgcggca
      601 cgccatgatc gcatcgcgca ccgcgatcgg ccggtcgagc tcgatccgca gcgactgctt
      661 gaccttgtcg taggcgtact ccgcgccggt gccgtgggcc tcgagcccga cggcatcgat
      721 gcaggcgtcg gggccgcgcc cgccggtgag gtccatcagc gcctcctgca cgctcggcac
      781 ctcctcgtag ttgatcacgt cggtcgcccc ggccttcttg accgcgaggt cgagtcggta
      841 cgcgaagcga tcgatcgcga tcaccttctc ggcgccgagc atccgcgcgc tggcgatcgc
      901 gaactggccg accggtccgg cgccccacac cgcgatgacc ttgcgcgggt tgatctcgca
      961 gaagtcggcg gccatgtagc cggtcgggaa gatgtcggac aggaacagga cctgctcgtc
     1021 cttcagcgcc gacggtacct tgagcggacc gaagtcggcg tacggcacgc gcacgtactc
     1081 ggcctggccg ccggcgaacc cgccggtcag gtgggcgtag ccgaacgccc cgcagatcga
     1141 atggcccatc agcttctcgc tgatccaggc gttcgggttg gcgttctcgc acaccgagaa
     1201 gttgccgagc tgacactcct cgcagttgcc gcacgcgatc gcgaacggca ccacgacgcg
     1261 gtcaccgacc tgcaagttct tgaccgcgct gccgagctcg acgacctcgc ccatgaactc
     1321 gtggccgagg atgtcgccgc gctgcatcgt cggcacgaag ccgttgtaga ggtggaggtc
     1381 cgagccgcag atcgtcgtcg aggtgacccg cacgatcgcg tcgcgcgcgt tgaggatccg
     1441 cggatccggc acctgctcga cgctgacctt gcgcttgccc atccagacgt tggccttcac
     1501 ggcgataccc ccttggccgc ggtcggcggg ctctcgagga acacccgcgg cggcgagatc
     1561 tcgtggcggg ccggcgatgg ctgcgccggg tggggcaggc gatacacgct ggcatccgag
     1621 cgcagcacct cgccggtctc cagcacctgc ttgagccgcc gcagatcgcc cttgacctgc
     1681 ggccgggcga ccagccgcgc cagcccgatc gacggctcgc ggccgagcac gccgagctgc
     1741 atctcgatcc ggacctcggt catgtcgcgc cccggcgccc gggtgaacgt cacccggccg
     1801 cgggtctgga cctggctgcc ctcgaccgag cgccacgaca gcacctgacc ggggcgatcc
     1861 tcgacgagct ccgtctccca gcgcaccgtg ccaacgaccg gcagccgggc ggtccaggtc
     1921 gagcgccggt tggcatgctg ctcgaccgac accaggtggt tcatgaacag gggcagctgc
     1981 tcgagccgcc ggcagaactc gtagacctcc atcggaggct tgttgatcgt caccgccgcg
     2041 atcaccggca gcgggtgccg ggcacgtccc tggcggcgcg ccgcgagcac gtcgagcgcc
     2101 gtcaccccgg cgaccatgcc gagcgccatg ccgagccggc gcttgccggt cccgggagtc
     2161 gtcagcgcca gcccgagcag gccgaggtcg atcgcgtcgc cgatcacccg ggcccagcgc
     2221 ggcgacggat gatccggacg gaggagcacc gcggagctcg atgcgatctc gcgcaccccc
     2281 atcgcgcgca ggatcgccgg cgccgcgccg tcggggtcga cgccgatcgc gctcgcgagc
     2341 agcctgggaa tcaccagctc ggtcacgccc agggtgacgc cgagccagcc gagccctcgg
     2401 atcaaactgg tcgcatctgc cttgtctggg gcgggtggct cgctctggac cacagagatc
     2461 tcattcatga tcgctccttg gaaatggccc tgaggaaggg ccgttgtcgg acagacgcga
     2521 tcaagcaacg cgtgttccac gcgggcctcg cgatcccggc gcacggtgat cgggaatgct
     2581 cgtgttcgac gccgaccgcg cgggcgcgca cggcgcggcg gcatgtccga tgcactcgat
     2641 cggcgtgaag gagatcccca tgccaaatcg tatggattcg atgttgtcca agggcgcagg
     2701 gatgatgaag ggaatgaagg cccgcctcga aggactcacc ggcgtgttca agacgctcgc
     2761 cgagcagcac ggagaggtgg cggcgatgct caagcggctg cagagcaagc ccgagaagaa
     2821 gcccgagctg tggccggaga tccggcgcga gctgctgtcg cacgagcgcg gcgagatccg
     2881 cgaggtctac cccgtgctgc gccagcacgt gcagacccgc gcgcttgccg agcaccacga
     2941 tcaggaggcc gccgagatcg agcacctgat cgagcgggtc gacgccgcga cggccgacga
     3001 ctggcgcccg ctcttcgacc agctcgtgga gaccgtgatc caccacgcca acgaggagga
     3061 gcacaacatc ttccccaagg cgcaggaggc gatcggcgag caggccgcca aggacctcga
     3121 gccccggttc ctcgccgcca agaagcaggt catggccacc gcctagccgg cgcgccgcga
     3181 gcgggtgccg gccgccgcgc cgggctgctc gccgtcgacc agccgggcga acgcccgcgc
     3241 gttgcgcacc
//
LOCUS       DASVOR010001469         2745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_15804,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001469 DASVOR010000000
VERSION     DASVOR010001469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15804"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..738)
                     /locus_tag="VF516_27785"
     CDS             complement(<1..738)
                     /locus_tag="VF516_27785"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEX8111575.1"
                     /translation="MTALARGLEVLACFRSGDTLLGNGDLAERCRLPKSTVSRLSQTL
                     ARLGYLRYVDDVAKYRLGGATLALGSAMLSGMAIRQLARPMMQEVADFSQSVVALGIR
                     VGLGVIYIEVCRNKAALTLSLDVGSWVPLATTAIGRACLAVAADDERRDLMAQLRAAD
                     PAAWPKRKRQIDAAIEQHRKIGCCSSFGEWQPDVSGVAIGFRPGRGLPAMALNCGGPS
                     FSLSRAFLLGEVRPRLASVVRRLEESLG"
     gene            1074..1907
                     /locus_tag="VF516_27790"
     CDS             1074..1907
                     /locus_tag="VF516_27790"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111576.1"
                     /translation="MTADRSPDDMGIYRTAPDGGDPTAPLLRIRGVTVRFGSIVALSD
                     VSFEVRRGEILGLIGPNGAGKTTLFNCLSRLCEVERGSIELGGRSLLEVPRHDIARVG
                     IGRTFQHLGLFNTMSVTDNVLVGTHVRERGGFWADALRIRSLVHGERRARERVRQLIE
                     RLDLGAVAHTPVGGLPFPVKKRVELARALAAEPVLLLLDEPAGGLNHEEVEHLGELVK
                     MIRDTFGVTILMVEHHLNFVMRVSDRVVALDFGRTLAEGTPAEMQAHPEVIRAYLGAP
                     T"
     gene            1904..2617
                     /locus_tag="VF516_27795"
     CDS             1904..2617
                     /locus_tag="VF516_27795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111577.1"
                     /translation="MSGLLVLEDVDASYGATTVLRGVSLEVPDGGMVALLGANGAGKT
                     TTLRAICNMGIKTRGGIRFAGKCIDRMATEDIVRLGVGHVPEGRGTFMSLSVDENLRL
                     GALRRRDARIAGDIARMCDYFPKLRDRMDQQAGTLSGGEQQMLAIARALMARPRLLLL
                     DEPSFGLAPLIVQEIFRNLRVINKEERMSILLVEQNARLAFDLVDQVFLLETGRVVAS
                     GPPQQIAADETVRRAYLGQ"
BASE COUNT          412 a          947 c          989 g          397 t
ORIGIN      
        1 gccgagcgat tcctcgagcc ggcggaccac gctggccagc cggggccgga cctcgccgag
       61 caggaacgcg cgcgacaagc tgaacgacgg gccgccgcag ttgagcgcca tcgcgggcag
      121 cccgcggccc ggccggaacc cgatcgcgac gccgctgacg tcgggctgcc actcgccgaa
      181 cgagctgcag cagccgatct tgcggtgctg ctcgatcgcg gcatcgatct ggcgcttgcg
      241 cttcggccag gccgccgggt cggcggcgcg cagctgggcc atcaggtccc ggcgctcgtc
      301 gtcggcggcg accgccaggc aggcgcggcc gatcgccgtc gtcgccagcg gcacccagga
      361 cccgacatcc aggctgaggg tcagcgcggc cttgttgcgg cagacctcta tatagatgac
      421 gccgaggccg acccggatgc cgagcgcgac cacggactgg gagaagtcgg cgacctcctg
      481 catcatcggc cgcgcgagct ggcggatcgc catgccggac agcatcgcgc tgcccagcgc
      541 cagggtcgct ccgccgagcc ggtacttggc gacgtcgtcg acgtagcgca ggtagcccag
      601 ccgggcgagg gtctgggaca gccgcgagac ggtcgatttc gggagccggc agcgctcggc
      661 gaggtcgccg ttgcccagca aggtgtcgcc ggaccggaag cacgccagga cctccaggcc
      721 gcgggccagc gcggtcacga agtggcgatc ctccttggct cgcggtctcc cccgcggcag
      781 gcggcgcgga gtctgcgaat tacgcgatga cacgaccatc cttggagcgg attatgcggt
      841 ggtgacggcc cggctcggaa gcccttcgcg gggggaatgg cccatggctg tcccgcgctt
      901 ggcgtgcgca tttgcaacgc agcgcggcgc gacgcgtacg acacatagga tcagcttgac
      961 ccgtcggagt cactcgtcta gcttcgaggc cggattgcgg ttgcagaacg tagttctgca
     1021 atgcagcacc ggatcgccgc tctgacaagg ctgccaagga caggacacgt gatgtgaccg
     1081 cagatcgctc gcccgatgac atggggattt accggacggc gccggacggg ggagatccga
     1141 cggcgccgtt gctccggatc cgcggagtca ccgtgcggtt cggctcgatc gtggcgctga
     1201 gcgacgtgtc gttcgaggtc cggaggggcg agatcctcgg attgatcggc cccaacggcg
     1261 ccggcaagac cacgttgttc aactgcctga gccggctatg cgaggtcgag cgcgggtcga
     1321 tcgagctcgg cggccgctcg ctcctggagg tcccccgcca cgacatcgcg cgggtcggga
     1381 tcggccgtac tttccagcac ctcgggctgt tcaacaccat gtcggtcacc gacaatgtgc
     1441 tggtcggcac ccacgtccgg gagcgcggcg ggttctgggc cgatgcgctc aggatccgga
     1501 gcctggtcca cggcgagcgc cgggcgcggg agcgcgtccg ccagctgatc gagcggctcg
     1561 acctcggcgc ggtcgcgcac accccggtcg gcggcctgcc gttcccggtc aagaagcggg
     1621 tcgagctcgc gcgcgcgctg gccgccgagc ccgtgctgct gctgctcgat gagccggccg
     1681 gcgggctcaa ccacgaggag gtcgagcacc tcggcgagct ggtcaagatg atccgcgaca
     1741 cattcggcgt gacgatcctg atggtcgagc accacctcaa cttcgtgatg cgggtgtccg
     1801 accgggtggt cgcgctcgac ttcggccgca ccctggccga gggcactccc gccgagatgc
     1861 aggcccaccc cgaggtgatc cgggcctacc tgggagcgcc gacatgagcg ggctgctcgt
     1921 gctcgaggac gtcgacgcca gctacggcgc gaccaccgtg ctgcgcgggg tctcgctcga
     1981 ggtccccgac ggcgggatgg tcgccctgct cggcgccaac ggcgccggca agaccacgac
     2041 gctgcgcgcg atctgcaaca tggggatcaa gacccgcggc gggatccggt tcgccggcaa
     2101 gtgcatcgac cggatggcca ccgaggacat cgtgcggctc ggcgtcggcc acgtcccgga
     2161 gggccgcggc acgttcatga gcctgtccgt cgacgagaac ctccggctcg gcgcgctgcg
     2221 ccggcgcgac gcccggatcg ccggtgacat cgcccggatg tgcgattact tccctaagct
     2281 gcgcgaccgg atggatcagc aggccgggac gctgagcggc ggcgagcagc agatgctcgc
     2341 gatcgcgcgc gcgctgatgg cgcggccgcg gctgctgctg ctcgacgagc cgtcgttcgg
     2401 gctcgcgccg ctcatcgtcc aggagatctt ccggaacctg cgggtcatca acaaggagga
     2461 gcggatgagc atcctgctgg tcgagcagaa cgcgcggctc gccttcgacc tggtcgacca
     2521 ggtgttcctg ctcgagaccg ggcgcgtggt cgcgagcggg ccgccgcaac agatcgccgc
     2581 cgacgagacc gtccggcgcg cctacctggg gcagtgaccc tcatgcaggc cttcctccat
     2641 cagctgctgt ccggcatcgc cgacggcgcg atctacgcca gcgtcgcgct cgcgctggtg
     2701 atgatcttca aggcgaccca ccaggtgaac ttcgcgcagg gcgag
//
LOCUS       DASVOR010001470         2074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_453183,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001470 DASVOR010000000
VERSION     DASVOR010001470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453183"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="VF516_27800"
     CDS             <1..847
                     /locus_tag="VF516_27800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111578.1"
                     /translation="YIQSPEKLLASFPMQIEAYGRAAFGAFSLQVDVGARESETGNEA
                     ATHAWSREHYLTWQQKPGENTGLYVRVGRFMPVFGLRLAEHLTYIRRWGGTPLYGETY
                     GLAVEYVDPRFEVHATGFIQDPILDTPEHSSGGAALGELRLSERLSLGAEGMYTQSDD
                     DKKVRIGALAKLYLDGPGLLLQLEGQFMNQLIEPRGAPKQIIAYLLASRFFDPAFLLD
                     LGLGFFDENIQITKLHRECIDLNVHWFTTSHVEAIFTGRFETLAFGAAGPNAGYALVQ
                     LHYRL"
     gene            872..1999
                     /locus_tag="VF516_27805"
     CDS             872..1999
                     /locus_tag="VF516_27805"
                     /inference="COORDINATES: protein motif:HMM:NF019042.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEX8111579.1"
                     /translation="MTRVWGAIVLAAAVSAAVSAASWPRAALADPAPAFTWQPFGYLR
                     AQYIAVQDDPNVAFVGRDDGFELQNARIGVRGELDRRVGFVIAFDGAIDERTQVNSPQ
                     GKLGVGLRDAFADVALAGKLAVRAGFFQAVVDPQSLVADTARELVDQPIESRGMRATE
                     GYQTAGLPPGRSLGVALRLDPEPPAEGGIGLGFELAVQNGADEFSSNNDNDKPAVSAA
                     VIARLSPGSWVLAAARYNPRTVGDLPFRQDEDDLQGTAGLRLALGPVMLDGGLIVQRT
                     TFPTTGGPSQDAYGGHAQAMVALPVSLPGASPLWVGYRFGILDPSSLITTDRVMEHTA
                     GAVLGLPRYRMRVQLQIVHVVEQAARELRNDRAQLAAELVL"
BASE COUNT          272 a          767 c          729 g          306 t
ORIGIN      
        1 ctacatccag tcgcccgaga agctgctcgc gagcttcccg atgcagatcg aggcctacgg
       61 tcgggcggcg ttcggcgcgt tctcgctgca ggtcgacgtc ggcgcgcgcg agtccgagac
      121 cggcaacgag gccgccaccc atgcgtggtc ccgcgagcac tacctcacgt ggcaacagaa
      181 gccgggagag aacaccgggc tgtacgtgcg cgtcgggcgg ttcatgccgg tgttcgggct
      241 gcgcctggcc gagcacctga cgtacatccg gcgctggggc ggcaccccgc tgtacggcga
      301 gacctacggg ctcgcggtcg agtacgtcga tccccggttc gaggtccacg cgaccggctt
      361 catccaggat ccgatcctcg acactccgga gcactcgagc ggcggggcgg cgctcggtga
      421 gctccggctg agcgagcggc tctcgctggg cgccgagggt atgtacaccc agtccgacga
      481 cgacaagaag gtccggatcg gcgcgctcgc caagctctac ctcgacggcc ccggtctgct
      541 gctgcagctc gagggccagt tcatgaacca gctgatcgag ccgcgcggcg cgcccaagca
      601 gatcatcgcg tacctcctgg cgtcgcggtt cttcgatccg gcgttcctgc tcgacctcgg
      661 cctgggcttc ttcgacgaga acatccagat caccaagctg caccgcgagt gcatcgacct
      721 caacgtccac tggttcacga cctcgcacgt cgaggcgatc ttcaccggcc ggttcgagac
      781 cctggcgttc ggcgcggccg gccccaacgc cggctatgcg ctggtccagc tgcactatcg
      841 actgtgagcc ggcggcgggc taccaatgcg catgacccgg gtgtggggtg cgatcgtcct
      901 ggccgccgcc gtctccgccg ccgtctccgc cgcctcctgg ccgcgcgccg cgctggccga
      961 tcccgccccg gcgttcacct ggcagccgtt cggctacctg cgcgcccagt acatcgcggt
     1021 ccaggatgat cccaacgtcg cgttcgtcgg ccgcgacgac ggcttcgagc tgcagaacgc
     1081 gcggatcggc gtgcgcggcg agctcgatcg ccgcgtcggg ttcgtgatcg cgttcgacgg
     1141 cgcgatcgac gagcggaccc aggtcaactc gccgcagggc aagctcggcg tcggcctgcg
     1201 cgatgcgttc gccgacgtcg cgctcgccgg caagctggcc gtgcgcgccg ggttcttcca
     1261 ggccgtggtc gacccccagt cgctggtggc cgacaccgcg cgcgagctgg tcgaccagcc
     1321 gatcgagagc cgcggcatgc gcgccaccga gggctaccag acggccggtc tgccgccggg
     1381 gcgctcgctc ggcgtggcgc tccggctcga ccccgagccg ccggccgagg gcggaatcgg
     1441 gctgggcttc gagctcgcgg tgcagaacgg cgccgacgag ttctcgtcga acaacgacaa
     1501 cgacaagccg gccgtgtcgg cggcggtgat cgcccggctg tcgcccggca gctgggtcct
     1561 ggccgccgcg cgctacaacc cgcgcacggt cggcgacctg ccgtttcgcc aggacgagga
     1621 cgacctccag ggcacggcgg ggctgcggct cgcgctcggc ccggtgatgc tcgacggcgg
     1681 cctgatcgtc cagcgcacga cgttcccgac caccggcggg ccgtcccagg acgcgtacgg
     1741 cggccacgcc caggcgatgg tggcgctgcc ggtctcgctg ccgggcgctt caccgctctg
     1801 ggtcggctac cggttcggca tcctcgaccc gtcgagcctg atcaccaccg accgcgtcat
     1861 ggagcatacc gccggcgcgg tgctcggcct gccgcgctac cgcatgcggg tgcagctcca
     1921 gatcgtccac gtggtcgagc aggccgcgcg cgagctgcgc aacgaccggg cgcagctcgc
     1981 cgcggagctc gtgctgtgag ggccgcggtc gcggtcgccg tgcttgccgg cgccgggctc
     2041 atggccccgg ccggctgcgg cgatccggcg ccgc
//
LOCUS       DASVOR010001471         5746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_44480,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001471 DASVOR010000000
VERSION     DASVOR010001471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5746
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44480"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VF516_27810"
     CDS             <1..516
                     /locus_tag="VF516_27810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111580.1"
                     /translation="ALGPVRANVAVTMGKARTQISAAHTDRTLPGSSPPITFTTVTFT
                     LWRLQEDPPAVLPGPCPSYPCWVAVESYTTDPNVDVESWAPDVVDHTSPARTNDWTSW
                     AFGPGSPTNPPLQCRPDGTCTPFSFFFKGVFPGPSCDPTVPANQPLHEQCGKLAVMPL
                     GGAITTRADWN"
     gene            597..1181
                     /locus_tag="VF516_27815"
     CDS             597..1181
                     /locus_tag="VF516_27815"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HEX8111581.1"
                     /translation="MMASALVLAAGCPSTLPKYDYSKEPDPRSRELVLGVGDSISINV
                     WENKEFNTDATIRPDGTITMPLIGDIKAVGETPSSLKARIKSRLGDYVKLGTSAEITV
                     AVRSWKSYRFTIQGEVAHPGVIGADQYLTVADAMALAGGPTKFAKRNEITLLRRDPKS
                     GEIRKIPLDYDLVASGKRQDMNIYVLPGDTIWVP"
     gene            complement(1254..1616)
                     /locus_tag="VF516_27820"
     CDS             complement(1254..1616)
                     /locus_tag="VF516_27820"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8111582.1"
                     /translation="MATVLGIGGVFVRSRDPDALRAWYRDLLGLDIQDWGGAQFGSTP
                     ETYGVWSAFRADTTYFAPSEREFMVNLRVDDLEGLLARLREGGARVLDRREDSADGKF
                     GYVVDPDGTLLELWQPPG"
     gene            1788..2825
                     /locus_tag="VF516_27825"
     CDS             1788..2825
                     /locus_tag="VF516_27825"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEX8111583.1"
                     /translation="MSVIASVSPSVAAAPSGRLSPELCLKIYDLMLRTRVLEDRLITM
                     YKQGDGYFWIGGPGEEAFSVPLGLLVDKGQGLDHDYLHLHYRCAGTLLAMGADPVDSL
                     RQMRNAVTDPYSRGRNFAGHYTFRKWNMVPVRSPIATQWAIAIGTARAQRAGTGAKGI
                     TIVQGGDAGTAEGDFASALVWSTRRGQELPLLMIVCNNRYGISTPYAGQHGYDQISDR
                     GKAFGMPTAVIDGNDPEASYTALQQAIDYVRTERRPYLLEAMVSRLRGHSSASGANLV
                     KGEVDCLERFERVLEDRQLITRAGMDELRERYTAELAEAHKRVRTEPHPLPSSVWDYI
                     FADKNLVCGEG"
     gene            2836..3888
                     /locus_tag="VF516_27830"
     CDS             2836..3888
                     /locus_tag="VF516_27830"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8111584.1"
                     /translation="MIQAIRMALHIGESRLGVTDIFGEDVGPPLGGVFTGTQGLETAW
                     NTPLDERGIVGTAVGLALAGQRPVAEIQFCDYVFNTIDLLKIAGNIYWSSAGDWNVPM
                     VLMTPVGAGIRGSIYHSHSFDAGATRLPGWKIVMPSTPRDAYGLMLSAIVDPNPVMYL
                     MPKALLRVKAEAGEEIPGEPGDDKALSGMIDAPLGDRSGWKPRWPELEELYIPIGEAR
                     IARAGTGVSVLTYGRNVAMAVAAANELAAEGIEAEVVDLRSLHPYDWAAIVASVRKTH
                     RVLCVNEDTEITNFGEHLIRRIIEELFYELYAPPKLLAGGHLPGIGLADSLEMASVPQ
                     KDGIKAAIAELARHEP"
     gene            complement(3895..5463)
                     /locus_tag="VF516_27835"
     CDS             complement(3895..5463)
                     /locus_tag="VF516_27835"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8111585.1"
                     /translation="MLSTTELGRNDSPRTGVAGESARRCEPTGTALLFHPPGLLQDAM
                     SPVEQAACALRRACSLVALALACGAPQPASTAEHGAPTLVIAHVTVIDPRDGSALPDR
                     TVAIRDDRIASVTASRATGVHPGARTVDATGKFMIPGLWDMHVHTFFGTWVPGGKEVT
                     LPLFIASGVTGVRDMGSEIEPILDARAAIAGHRMLGPRMVISGPMLDGPHTQFPASIA
                     IATPDDGRRAVQVLAERGVDFIKIQSYVPRDAYFAIAEECQRRGLVFVGHVPDAVRGA
                     EAARSGQKSFEHLIGIFEGSSTAEDALLAGGKTPGKFLATYDADKEAALIRLLVGRQI
                     WQCPTLYWERGQWLVDAIDVTRDPDAGYAPVSWRERSWPRFTTSILRELDTDPLDVRR
                     RFVDHELGIVRRLHAAGVPLLAGTDTPAGVDVIPGVSLHRELARLVDAGLTPLAALQT
                     ATINPARFLGRLAELGTVEPGKLADLVVLDRDPRLDIANTRAIAAVVAAGRYLPRDEL
                     DAMLRGVAAAARDL"
BASE COUNT          902 a         2016 c         2002 g          826 t
ORIGIN      
        1 gcgctcggtc cggtgcgcgc caatgtcgcg gtcacgatgg gcaaggcgcg gacccagatc
       61 tccgccgccc acacggaccg cacgctgccg ggctcgtccc cgccgatcac gttcacgacc
      121 gtcaccttca cgctgtggcg gctgcaggag gatccaccgg ccgttctccc cggtccatgc
      181 ccctcgtacc cgtgctgggt cgccgtggag agctacacca ccgaccccaa cgtcgacgtc
      241 gagagctggg cgcccgacgt tgtcgaccat acctcccccg cacggaccaa cgactggacg
      301 agctgggcgt tcgggcccgg cagcccgacc aacccgccgc tgcagtgccg gcccgatggc
      361 acctgtactc cgttctcctt cttcttcaag ggcgtgttcc caggcccgag ctgtgacccg
      421 acggtgccgg ccaaccagcc gctccacgag cagtgcggca agctcgccgt catgccgctc
      481 ggcggcgcga tcaccacgcg cgcggactgg aactaggggc tgcgccgggc gacactgcgc
      541 tcgtgatgcg cgcagcgact cttcgcctcc cgatcggccg agcggccctc cacgcgatga
      601 tggcgagcgc cctggtgctc gccgccggct gtccctccac cctgccgaag tacgattaca
      661 gcaaggagcc cgaccctcgc tcccgcgagc tcgtgctcgg cgtcggcgac tcgatctcga
      721 tcaacgtgtg ggagaacaag gagttcaaca ccgatgcgac gatccgtccc gacggtacga
      781 tcaccatgcc gctgatcggc gatatcaagg cggtgggcga gaccccgagc tcgctcaagg
      841 ccaggatcaa gagccggctc ggggactatg tcaagctcgg caccagcgcc gagatcaccg
      901 tcgcggtgcg gtcgtggaag agctatcgct tcacgatcca gggcgaggtc gcccaccccg
      961 gcgtgatcgg cgccgaccag tacctgaccg tcgccgatgc gatggcgctc gccggtgggc
     1021 ccaccaagtt cgccaagcgc aacgagatca ccttgctgcg acgcgatccc aagtccggcg
     1081 agatccgcaa gatcccgctc gactacgacc tcgtcgcgtc ggggaagcgg caggacatga
     1141 acatctacgt cctgccgggc gacacgatct gggtgccctg atcgggcgat ggccagaggc
     1201 agcgggccga ggcagcgggc cgagggtcgc tgtcctcgcc gatcgtcacg tcgtcagccg
     1261 ggaggctgcc acagctccag cagcgtgccg tccgggtcga ccacgtagcc gaacttgccg
     1321 tctgcgctgt cctcgcggcg atcgagcacg cgggcgcccc cctcgcgcag ccgggcgagc
     1381 aggccctcga gatcgtcgac ccgcaggttc accatgaact cgcgctcgct gggcgcgaag
     1441 taggtcgtgt cggcgcggaa cgcgctccac acgccgtagg tctccggcgt cgacccgaac
     1501 tgcgcgccgc cccagtcctg gatgtcgagg ccgagcaggt cgcgatacca cgcgcgcagc
     1561 gcgtcgggat ctctcgatcg cacgaacacg ccgccgatgc cgagcacggt tgccatggcg
     1621 gcaacgtatc cggccggcag ccgctcgtcg atcgcgaagg ctcgcgcaat gcgtcgcagc
     1681 cgcgatgctg caccgcgtag caggcgcagc ccgttcggcc ggtcggcacc ggaaactccg
     1741 gaccggacga tgctggcgcg cggcgattgc ggtaagacgg tcccgccatg agcgtcatcg
     1801 ccagcgtttc cccttcggtc gccgccgcgc cgtccgggcg cctgtctccg gagctgtgcc
     1861 tgaagatcta cgatctcatg ctccggaccc gggtcctcga ggaccgtctg atcaccatgt
     1921 acaagcaagg tgatggctac ttctggatcg gcggccccgg cgaggaggcg ttcagcgtcc
     1981 cgctcggcct gctggtcgac aagggccagg gcctcgacca cgactacctg cacctgcact
     2041 accgctgcgc cggcacgctg ctcgcgatgg gcgccgatcc ggtcgactcg ctgcgccaga
     2101 tgcgcaacgc ggtgaccgat ccgtactcgc ggggccgcaa cttcgcgggg cattacactt
     2161 tccgcaagtg gaacatggtg ccggtccgat cgccgatcgc gacccagtgg gcgatcgcga
     2221 tcggcaccgc gcgcgcgcag cgggccggca ccggcgccaa gggcatcacg atcgtgcagg
     2281 gcggtgacgc cggcacggcc gagggcgact tcgcgagcgc gctggtgtgg agcacgcggc
     2341 gcggccagga gctgccgctg ctgatgatcg tgtgcaacaa ccgctacggg atctcgacgc
     2401 cgtacgccgg ccagcacggc tacgaccaga tctccgaccg cggcaaggcg ttcggcatgc
     2461 cgaccgcggt gatcgacggc aacgatccgg aggcgagcta caccgcgctc cagcaggcga
     2521 tcgactacgt gcggaccgag cgccggccgt acctgctgga ggcgatggtg tcgcggctgc
     2581 gcggccactc gtcggcgagc ggcgccaacc tggtcaaggg cgaggtcgac tgcctggagc
     2641 ggttcgagcg tgtgctggag gaccgccagc tgatcacccg ggccggcatg gacgagctgc
     2701 gcgagcgcta caccgccgag ctcgccgagg cccacaagcg ggtccggacc gagccgcacc
     2761 cgctgccgag ctcggtgtgg gactacatct tcgccgacaa gaacctggtc tgcggggagg
     2821 gctgagatgg cgacgatgat ccaggcgatc cgcatggcgc tgcacatcgg cgagtcgcgg
     2881 ctcggcgtca ccgacatctt cggcgaggac gtcggcccgc cgctcggcgg tgtgttcacc
     2941 gggacccagg ggctggagac cgcgtggaac accccgctcg acgagcgcgg catcgtcggc
     3001 accgcggtcg gcctcgcgct ggccggccag cggccggtcg ccgagatcca gttctgcgac
     3061 tacgtgttca acacgatcga tctgctgaag atcgccggca acatctactg gtcgtcggcc
     3121 ggcgactgga acgtgccgat ggtgctgatg acgccggtcg gcgccgggat ccgcggcagc
     3181 atctaccact cgcactcgtt cgacgcgggc gcgacccggc tgccggggtg gaagatcgtg
     3241 atgccgtcga cgccgcgcga cgcctacggc ctgatgctga gcgcgatcgt cgaccccaac
     3301 ccggtgatgt acctgatgcc caaggcgctg ctgcgggtca aggccgaggc cggcgaggag
     3361 atccccggcg agcccggcga cgacaaggcg ctgtccggca tgatcgacgc gccgctcggc
     3421 gaccgcagcg ggtggaagcc gcgctggccg gagctcgagg agctgtacat cccgatcggc
     3481 gaggcgcgga tcgcgcgggc cggcaccggc gtcagcgtgc tgacctacgg ccgcaacgtg
     3541 gcgatggcgg tcgcggccgc gaacgagctc gccgccgagg gcatcgaggc cgaggtcgtc
     3601 gatctgcggt cgctgcatcc ctacgactgg gcggcgatcg tcgcgagcgt gcgcaagacc
     3661 caccgcgtgc tgtgcgtcaa cgaggacacc gagatcacca acttcggcga gcacctgatc
     3721 cggcggatca tcgaggagct gttctacgag ctgtacgcgc ctccgaagct gctcgccggc
     3781 ggccacctgc ccgggatcgg gctcgccgac agcctcgaga tggcgagcgt gccgcagaag
     3841 gacggcatca aggctgcgat cgccgagctc gcgcgccacg agccgtaggg ttcatcagag
     3901 atcccgggcc gcggcggcga cgccgcgcaa catcgcgtcc agctcgtcgc gcggcaggta
     3961 gcggcccgcc gcgaccaccg cggcgatcgc gcgggtgttg gcgatgtcga gccgcggatc
     4021 gcggtcgagc acgaccagat cggcgagctt gccgggctcg accgtgccga gctcggcgag
     4081 ccggccgagg aaccgggccg ggttgatcgt cgcggtctgc agcgcggcga gcggcgtgag
     4141 cccggcgtcg accagccgcg cgagctcgcg gtgcaggctc acgcccggga tcacgtcgac
     4201 gccggccggg gtgtcggtgc cggcgagcag cggcacgccg gcggcatgga gccggcgcac
     4261 gatgccgagc tcgtggtcga cgaaccgccg ccggacgtcg agcggatcgg tgtcgagctc
     4321 gcgcaggatc gaggtggtga accgcggcca gctgcgctcg cgccacgaca ccggcgcata
     4381 gcccgcgtcg ggatcgcggg tgacgtcgat cgcgtcgacc agccactggc cgcgctccca
     4441 gtacagcgtc gggcattgcc agatctggcg gccgaccagc agccggatca gcgcggcctc
     4501 cttgtccgcg tcgtaggtcg cgaggaactt cccgggagtc ttgccgcccg cgagcagcgc
     4561 gtcctctgcg gtcgagctgc cctcgaagat gccgatcagg tgctcgaagc tcttctgtcc
     4621 ggacctcgcg gcctccgccc cgcgcaccgc atcgggcacg tggccgacga ataccaggcc
     4681 gcggcgctgg cattcctcgg caatcgcgaa gtaggcgtcg cgcgggacgt aggattgaat
     4741 cttgatgaag tcgacaccgc gctcggccag cacctgcacg gcgcggcggc cgtcgtcggg
     4801 cgtggcgatc gcgatcgacg ccggaaactg ggtgtgagga ccgtcgagca tcggcccgga
     4861 gatcaccatc cgggggccga gcatccggtg ccctgcgatc gctgcgcgcg cgtccaggat
     4921 cggctcgatc tcgctgccca tgtcgcgcac gccggtgacg ccactggcga tgaacagcgg
     4981 gagcgtgact tccttgccgc cgggcaccca tgtgccaaag aacgtgtgga catgcatgtc
     5041 ccacaggccc gggatcatga acttccccgt cgcgtcgacc gtgcgcgcgc ccggatggac
     5101 gccggtggcc ctcgatgccg tcacggacgc gatccgatca tcccggatcg cgacggtccg
     5161 gtcgggcagg gcgctgccgt cccgggggtc gatcaccgtg acgtgcgcga tgacgagcgt
     5221 cggggcgccg tgctctgccg tcgacgctgg ttgcggcgca ccacaggcca gggccagggc
     5281 cacgaggcta caagctcgtc gcagggcgca agccgcttgt tcgaccgggc tcatggcgtc
     5341 ttgtaacaag ccgggcgggt ggaataaaag cgctgtcccg gtcggttcgc accggcgggc
     5401 ggattccccg gcgaccccgg tccggggtga gtcattcctc cccagttcag tcgtcgataa
     5461 cacgccagtc agcggtcagg acagccgact ggacccggtt cgggcgagtc gaaaattggt
     5521 ggccatgtcg gacgggtcga gtccgttcac accccccagg ctgcgcccgg acgctcggcg
     5581 tcgggggacg cctgggatca tatagagtgg gggcgcctcg caccggaaac caccgtgttg
     5641 tttcggtaaa cctggcaacc tgatcagtgc ttgtcggagt catgcgccat ccgggcgcca
     5701 aatgcaacca ggacagcgcc atttgacgaa cattcgcctt gactac
//
LOCUS       DASVOR010001472         4348 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_160938,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001472 DASVOR010000000
VERSION     DASVOR010001472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160938"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VF516_27840"
     CDS             <1..501
                     /locus_tag="VF516_27840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111586.1"
                     /translation="GGPAVALAPPIVVAIPAVPALAPGIAAAPMLMSARPDGMGDPND
                     YRGPGPYRDKFTNPDKQGELEAEHVPSKALTEQQAPGVNPQPVGDGPWFSNRLKGAET
                     KRIMEAWRAYERWVRDMALSQAEKERLIEEFWAHAREEDWNNHNGGGAIPVDPDELDL
                     LPAPGT"
     gene            complement(510..887)
                     /locus_tag="VF516_27845"
     CDS             complement(510..887)
                     /locus_tag="VF516_27845"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8111587.1"
                     /translation="MFDHVKFGVRDYAASKAFFLKALEPLGVAVVSEGLPTYGVELMP
                     PESKASLCLYQTEEKPAHLHLAFRAENRRQVEAFYRAALEAGGKDNGAPGLRPHYHAN
                     YYAAFVIGPDGHNIEVVCHEPEA"
     gene            complement(1014..2612)
                     /locus_tag="VF516_27850"
     CDS             complement(1014..2612)
                     /locus_tag="VF516_27850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8111588.1"
                     /translation="MKVGLFYRPRPTPSTIFGAGIAADDFVHSLLRHGTCAPQLFCAP
                     HDLEEAEARLGVRPRDERRLLDGRTARAIEVWHSPYGNSFEPLNLRSARRGGYHPITL
                     AHHSLSYQGLLHQWFLRLLVQDVQPFDAIVCSSTAARDAIAKIFARVADLAGREIGAR
                     LAFRGRLEVIPLGVDTERFAPGDPAAARARLGLPRTALLLLWLGRFSAHDKADLLPTL
                     RLFRDLVRANPKRALRLILAGTDSFTGESRVLDAYASALGVADRITILRGVSADDKPF
                     LYRAADVFLSPVDNVQEMFGITPVEAMACGIPQVVADWNGYRDLVAHGETGFRVATHW
                     ADCDDDAAQLSLLPNAHQRDHMTLALSVAVDFAQYRAFVQTLIDRPALRREMAAASRA
                     RAVACFGWKTVIARLEALWRELRRMADARPVRGGRDRYERPAFFATFAGHATHILGAD
                     ARVRLTRTGRDLIRGREILPTYHAAGGVLDERLLERLLAALASVRTARGASLGELSRR
                     AAKPDRALRHLMWLLKYGFVERAP"
     gene            3152..3304
                     /locus_tag="VF516_27855"
     CDS             3152..3304
                     /locus_tag="VF516_27855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111589.1"
                     /translation="MQKKPTRQLKILRLRKEALKVLNDEQQLRVHGGSDPWDTGQTAY
                     PICQNA"
     gene            3506..>4348
                     /locus_tag="VF516_27860"
     CDS             3506..>4348
                     /locus_tag="VF516_27860"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX8111590.1"
                     /translation="MAAPLRVSRVAAPVPEFRSCYVATRQPVLIDGLFDGQPIRRLAT
                     ARAAQRAIGDMDFVVQWNYTANLYYANAREAGQPERTTLANYLDYVALNPRTPRMCIE
                     QPIAPTLAALIEPPAICWNNERTLMFIGNAGNFSHLHFDGDHRQVLMYQVFGVKRVIL
                     IPPIAAKKLLPHQKFSSVCVQHMSERERRAFLRYVGGSECLVRPGQAIFMPALFWHHV
                     EYVETSLSVHFRFGQTKHGRFFDDFVHRSMYPQNVAACFQDEQFAAGPGHKVMRRLRR
                     AVAAT"
BASE COUNT          729 a         1458 c         1506 g          655 t
ORIGIN      
        1 ggcgggccgg ccgtggccct cgcgccgccg atcgtcgtcg ccatcccggc ggtgccggcg
       61 ctggcgcccg ggatcgccgc ggcgccgatg ctgatgagcg cgcggcccga cggcatgggc
      121 gatccgaacg actaccgggg accgggaccg taccgcgaca agttcaccaa cccggacaag
      181 cagggcgaac tcgaagcgga gcacgtgccg tcgaaagcgc tgaccgagca gcaggccccc
      241 ggcgtcaatc cgcagccggt cggcgacggc ccgtggttca gcaatcgact caagggcgcc
      301 gagaccaagc ggatcatgga ggcatggcgc gcgtacgaac ggtgggtgcg agacatggcg
      361 ctctcgcagg ccgagaagga acgcctgatc gaggagttct gggcgcatgc acgcgaggag
      421 gactggaaca accacaacgg cggcggcgcg atccccgtcg atccagacga gctcgatctg
      481 cttcccgcgc ctggaacctg acagtcgtgt caggcctcgg gttcgtggca aaccacttcg
      541 atgttgtgcc cgtccggacc aatgacgaag gctgcatagt agttcgcgtg gtagtgcggg
      601 cgcagaccag gcgcaccatt gtctttgcca cccgcctcca gagccgcgcg atagaaagct
      661 tcgacttgcc ggcgattctc ggccctgaag gccaggtgaa gatgcgccgg cttctcctcg
      721 gtctggtaca ggcacaatga agccttgctc tctggtggca tgagctcgac accgtacgtc
      781 ggaagcccct ccgagacaac agctacgccg agcggttcga gtgccttgag gaagaacgcc
      841 ttgctcgctg catagtctct gactccgaat ttgacgtggt caaacatgag ttctcccgaa
      901 gcgtgtgggc ctgcgcgagg cctgacgtgt tttcgacgac acccgatgtc gcctgacctg
      961 tcgcataaca cgctccttca tgtcacatga cacccgcgaa cggcatattc aggctaaggc
     1021 gcgcgctcca cgaaaccgta cttcagcagc cacatgaggt gccggagggc ccggtcgggc
     1081 ttcgccgcgc ggcgggagag ctcgccgagc gaagcgccgc gggcggtgcg gacggaggcc
     1141 agcgccgcca ggagccgctc gagcagccgt tcatcgagca cgccgccggc ggcgtggtag
     1201 gtggggagga tctcccggcc gcggatcagg tcgcgcccgg tgcgcgtcag gcgcacgcgc
     1261 gcgtcggccc cgaggatgtg ggtggcgtgg cccgcgaagg tggcgaagaa cgccggtcgc
     1321 tcgtagcggt cgcgcccacc gcgcaccgga cgcgcgtcgg ccatccggcg cagctcgcgc
     1381 cagagcgcct cgaggcgggc gatcacggtc ttccagccga agcaggccac ggcgcgcgcc
     1441 cgggacgccg ccgccatctc gcgccgcaac gccgggcggt cgatcaacgt ctggacgaac
     1501 gcgcggtact gcgcgaagtc gacggccacc gagagcgcca gcgtcatgtg gtcgcgctgg
     1561 tgggcgttgg ggagcaggct gagctgcgcc gcatcgtcgt cgcagtccgc ccagtgcgtc
     1621 gccacgcgga agccggtctc gccgtgcgcg accagatcgc ggtagccgtt ccagtcggcg
     1681 acgacctgcg ggatgccgca ggccatcgcc tcgacgggcg tgatgccgaa catctcctgg
     1741 acgttgtcca cgggcgacag gaacacgtcg gcggcgcgat agaggaaggg cttgtcatcg
     1801 gcgctcacgc cgcggaggat cgtgatgcga tcggcgacgc cgagcgcgct ggcgtacgcg
     1861 tcgagcaccc tcgactcacc ggtgaacgag tcggtgccgg cgaggatcag gcgcagcgcg
     1921 cgcttggggt tggcgcgcac gaggtctcga aacaggcgca gcgtcggcag gaggtccgcc
     1981 ttgtcgtgag ccgagaagcg gcccagccac aaaaggagca gcgcggtgcg cggcagcccc
     2041 agccgggcgc gcgccgccgc gggatcgccg ggcgcgaacc gttcggtgtc gaccccgagc
     2101 gggatgacct cgaggcgccc gcgaaaggcg aggcgggcgc cgatctcgcg gcccgccagg
     2161 tcggcgaccc gcgcgaagat cttggcgatc gcgtcccgcg ccgcggtgct cgaacagacg
     2221 atcgcgtcga acggctgcac gtcctggacc agcaagcgca ggaaccactg gtgcaggagg
     2281 ccctggtagc tgagcgagtg gtgggcgagc gtgatcgggt gataaccgcc ccgccgcgcg
     2341 ctgcgcaggt tgagcggctc gaacgagttg ccgtagggcg agtgccacac ctcgatggcg
     2401 cgcgccgtcc gcccgtcgag gagccggcgc tcgtcgcgcg ggcgcacgcc aaggcgcgcc
     2461 tcggcctcct cgaggtcgtg cggggcgcag aacagctgcg gcgcgcaggt cccgtgtcgc
     2521 agcagggagt ggacgaagtc gtccgcggcg atgcccgcgc cgaagatggt gctgggcgtc
     2581 ggccggggcc ggtagaacag cccgaccttc atcggcggcg ggccccgcgc gggtcgatcc
     2641 gacaggcgcg ggccggcggc ggcggatcgg ccccgggcga ggcgacgatc ctgctgcggc
     2701 agcgagcgtg cgcgagctcg aggtggtccg cgcccgcccg tacatggctc tgggagcgcg
     2761 cggccgcgtc gaagacgtgc agggcccgga gcacgcttga tcatatcgaa gttcgggcga
     2821 tgaagtaggc attcgccgtc tgcgtccggc agaagtcgca tgcggcacca gtcgtgctgc
     2881 gagacgcgct ctccgggtcc gggtaggaga accaagcgag gttcgggcgc tggcccgaga
     2941 tgcctcgatg tgatccgagg cggagccggc gagcaccacc ggatgccggt agtgtcagcg
     3001 ggcgtacgtt gggtcgacgt cggcgcagac ccgtccctgc cgagcgcgtt ggcggtcgtg
     3061 gcgaccacgg gttaccgagc ggtaggttgt aggcaataaa cctccatgag gacgccttta
     3121 cttcaataag agaaaccact accgtctcgc catgcaaaag aagccaacta ggcagctgaa
     3181 gatcctccgt cttcgcaagg aagccctcaa ggtcctcaat gacgagcagc agctccgtgt
     3241 acacggcggc agcgacccct gggacacggg gcaaacggct tacccgatct gccagaacgc
     3301 atgaattcga ccgatctgac gctgcgggtc aggcagtagc tctgcatcgc ttcgctccca
     3361 ccttccgtcg gcggactccc cctttgcggc actgcttgta cctgccgccc tgggtgctgg
     3421 cgcgcgacct tgcaggctgc agggttgacc ggtctcatcg tcgggaccga gccgcaccat
     3481 gaccgggtca tctcgaggag gctccgtggc tgcccctctc cgcgtctcgc gagtcgccgc
     3541 ccctgtaccg gaattccggt cgtgctatgt cgcgacccgc cagccggtgc tcattgatgg
     3601 tctgttcgat ggccaaccca tccgcaggct cgcgaccgcg cgcgccgcac agcgcgcgat
     3661 cggtgacatg gacttcgtgg tgcagtggaa ctacacagcg aacctctact acgcaaacgc
     3721 gcgggaagcg ggacagccgg agcgcacaac gcttgccaac tacctcgact acgtggcgct
     3781 gaacccgcgc accccgcgga tgtgcatcga gcagccgatt gcacccacgc tcgcggccct
     3841 gatcgagccc cccgccatat gctggaacaa cgagcgcacg ctcatgttca tcggcaatgc
     3901 cggtaacttc tctcatctcc acttcgacgg cgatcatcgc caggtgctca tgtatcaggt
     3961 attcggggtc aagcgcgtca tcctgatccc gccgatcgca gcgaagaagc tgttgccgca
     4021 ccagaaattc agctcggtct gtgtccagca catgtcggag cgcgagcgcc gtgcgttcct
     4081 gcgctatgtc ggcggctccg agtgtctggt tcggccgggc caggcgatct tcatgccggc
     4141 gctgttctgg caccacgtcg aatacgtcga gacgtcgctg tccgtccact tccgcttcgg
     4201 acagaccaag cacggtcgct tcttcgacga cttcgtccat cgcagcatgt atccccagaa
     4261 tgttgccgcg tgcttccagg acgagcaatt cgccgctgga cccggccaca aggtcatgcg
     4321 ccgacttcgc cgcgccgtgg ccgcgacc
//
LOCUS       DASVOR010001473         3783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_307492,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001473 DASVOR010000000
VERSION     DASVOR010001473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307492"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1326)
                     /locus_tag="VF516_27865"
     CDS             complement(<1..1326)
                     /locus_tag="VF516_27865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8111591.1"
                     /translation="MNPPFAASVHETGPPGFRFSSVFAILGRCMKRSPLLPIFLVVLV
                     DVLGFTIVYPLLPFYAEKFGASPLVATTLVSVYALCSLFSTPVIGRLSDQFGRRRLLL
                     ISQAGTCAGFLVLGFASALWMVFLGRILDGVTAGNLSTAQAYISDHTKPENRARAFGL
                     IGIAFGIGFFFGPALAGWLGHYGVHGAVPPGQSGELPFLVAAGLSVVSMICSYTLLEP
                     GITGSAAQPAGDAAAAPGGKRPAVFDLSIYLDYFRRPGLGSLYLQFFLFTFSFSAFMS
                     GFALFSERRFVTGAGHFWTSREVGYLFAYSGFLGIILQGGLIGRLVKRFGEPRLALAG
                     FVSVVVAYVVLGFVDVPPLAPLLVVTAISAFGNGVLRPVISSEITKQVGRHEQGVAIG
                     ISGSLNSLAMTIAPPIGGSLIGLAGPVEHLDPRWLVVWTLVPATAAAIGL"
     gene            1482..1781
                     /locus_tag="VF516_27870"
     CDS             1482..1781
                     /locus_tag="VF516_27870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111592.1"
                     /translation="MAMTAMIALLSGSLGCFGYNQSAKRWAYAGDAVLIVGGGTTIAL
                     DVTGKPPPCMPDAMNNGCSYKSSISGAMVAGAVLVAAGVFGILFNATRDNVKTSR"
     gene            complement(1789..2133)
                     /locus_tag="VF516_27875"
     CDS             complement(1789..2133)
                     /locus_tag="VF516_27875"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEX8111593.1"
                     /translation="MNRGEIWWADLAEPRGSEPGKRRPVLIVQEDSLTRSRLRTVMVV
                     PVTSNLKRAAAPGNVMLARSHSGLRIESVALACQVLTIDKEFLTARVAALPASVMARI
                     DAGLRLTLALGR"
     gene            complement(2130..2369)
                     /locus_tag="VF516_27880"
     CDS             complement(2130..2369)
                     /locus_tag="VF516_27880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111594.1"
                     /translation="MKTAISIPNDVFRRAERLAKQRKVSRSELYTSALIQMLEAEPKE
                     DLTRAYDAAFADDTSDRFTDTAARAALASVEWADE"
     gene            complement(2445..3176)
                     /locus_tag="VF516_27885"
     CDS             complement(2445..3176)
                     /locus_tag="VF516_27885"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111595.1"
                     /translation="MIRLVLGDVTAAQLRALATLVVGYGEGELRTTNEQNLVLRFVPD
                     ARLPALHRELVRVGLARKDANTVADVVSCPGASSCKIAVTASRGLGAQLTELLDRRPD
                     LIAGARGLDVKVSGCPNGCGQHYIAGIGFQGGMRKVAGRPAPQYLVYVGGGIRADGAD
                     FGRLLGKVPARRAAATLERLLDFYIASGAPGHAFWATVPIERLRALIADLGELSDADA
                     IEDDFIDLGETRAFEVVDGEGECAS"
     gene            complement(3173..>3783)
                     /locus_tag="VF516_27890"
     CDS             complement(3173..>3783)
                     /locus_tag="VF516_27890"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111596.1"
                     /translation="PLGDLTAAQFRALATLCVQFGEGEVRMTNEQNIALRWVPTTRLP
                     ALHRELVQVGLARKDANTVADVVSCPGASSCKIAVTASRGLGAQLTELLDRRPDLIAA
                     ARGLDVKVSGCPNGCGQHYIAGIGFQGGMRKVTGRPAPQYLVYVGGGIRADGADFGRL
                     LGKVPARRAAPRSPRSAASSPATPSGPRTTSGPRSSTGSPRS"
BASE COUNT          610 a         1319 c         1365 g          489 t
ORIGIN      
        1 caggccgatc gccgccgcgg tcgccggcac cagcgtccag acgacgagcc atcgcggatc
       61 gaggtgctcg acggggccgg ccagcccgat caagctaccg ccgatcggcg gcgcgatcgt
      121 catcgcgagc gagttcagcg atcccgagat gccgatcgcc acgccctgct cgtggcggcc
      181 gacctgcttg gtgatctcgc tggagatcac cgggcgcagc acgccattgc cgaacgccga
      241 gatcgccgtg accaccagca gcggcgccag cggcggcacg tcgacgaacc cgagcacgac
      301 gtacgccacg acgaccgaga cgaacccggc gagcgcgagc cggggctcgc cgaaccgctt
      361 gaccagccgg ccgatcagcc cgccctgcag gatgatcccg agaaaccccg agtacgcgaa
      421 caggtagccg acctcgcgcg aggtccagaa gtggccggcg ccggtcacga accggcgctc
      481 ggagaacagc gcgaagccgc tcatgaacgc cgagaacgag aacgtgaaca ggaagaactg
      541 caggtacagc gagcccagcc cggggcggcg gaagtagtcg aggtagatcg acaggtcgaa
      601 caccgcgggc cgcttgccgc ccggcgcggc ggccgcatct cccgcgggct gcgcggcgct
      661 gccggtgatc ccgggctcga gcagcgtgta gctgcagatc atcgagacca ccgacagccc
      721 cgcggcgacc aggaacggca gctcgccgct ctggcccggc ggcacggcgc cgtgcacccc
      781 gtagtggccg agccagccgg cgagcgccgg cccgaagaag aacccgatcc cgaacgcgat
      841 gccgatcagt ccgaacgcgc gggcgcggtt ctccggcttg gtgtggtccg agatgtaggc
      901 ctgcgcggtc gacaggttgc ccgcggtcac gccgtcgagg atccggccga ggaacaccat
      961 ccacaacgcg ctggcgaatc cgagcaccag gaaccccgcg cacgtcccgg cctggctgat
     1021 cagcagcagc cgccggcggc cgaactggtc cgacagccgg ccgatcaccg gggtcgagaa
     1081 cagcgagcac agcgcgtaca ccgacaccag cgtggtcgcg accagcggcg acgcgccgaa
     1141 cttctcggcg tagaacggca gcagggggta cacgatcgtg aaccccagca catcgaccag
     1201 gaccacgagg aagatcggca ggagcggcga ccgcttcatg cagcgcccga ggatggcgaa
     1261 aaccgacgaa aaccgaaatc ccggcgggcc ggtttcatgt acgcttgcag cgaatggggg
     1321 gttcacgcag cgcgttggcg gttgatcgag aggggacgtt cccgtcccct cttcgccggc
     1381 aaagccggcg aattcacccc tccgcgcgtc gcgtcgcgcg gccctgcacc tgtgcgtcct
     1441 cgctcgctcg atccgcgcct cggagatcct tgcatcagct catggcgatg accgcgatga
     1501 tcgccttgct cagtggttcc ctcggctgct tcggctacaa ccagtccgcc aagcgctggg
     1561 cctacgccgg cgacgcagtc ctgatcgtcg gcggcggcac gacgatcgcg ctcgacgtca
     1621 ccggcaagcc cccgccgtgc atgcccgacg cgatgaacaa cggctgctcc tacaagtcgt
     1681 cgatcagcgg cgccatggtc gccggcgcgg tgctcgtcgc cgccggcgtg ttcggcatcc
     1741 tgttcaacgc gacccgcgac aacgtcaaga cctcgcggta gtgcgcgatc agcgacccag
     1801 tgcgagcgtg aggcgcaggc cggcgtcgat gcgcgccatg acggacgccg ggagcgctgc
     1861 aacccgcgcc gtcaggaatt ccttgtcgat ggtcaatacc tggcacgcca gcgccacgct
     1921 ctcgatgcgc aagcccgaat gcgagcgcgc cagcatgaca ttaccggggg cagcagcgcg
     1981 cttgaggttg ctggtcaccg ggaccaccat caccgtgcgc agccggctcc gggtgagtga
     2041 atcctcctgg acgatcagga ccggccgtcg cttgcccggt tcggagccgc gcggttcggc
     2101 gaggtcggcc caccagatct caccgcgatt cactcgtcgg cccactcgac gctggccagc
     2161 gcggcgcgcg cggcggtgtc cgtgaatcga tcggaggtat cgtcggcaaa cgccgcgtca
     2221 taagcccggg tcagatcttc cttgggctcg gcttcgagca tctggatcaa cgccgacgtg
     2281 tagagctcgc tgcgtgacac cttgcgctgc ttggccaggc gctcggcgcg acggaacacg
     2341 tcgttcggga tcgagatcgc ggtcttcaca accgcgagta tgacacggtt atacctgggg
     2401 ggccaaatcg accatggggc gcagcgcgca agcgccgcgt accgtcagct ggcgcactcg
     2461 ccttcgccgt cgacgacctc gaacgcgcgg gtctcgccga ggtcgatgaa gtcgtcctcg
     2521 atggcgtcgg cgtcggacag ctcgccgagg tcggcgatca gggcgcgcag ccgctcgatc
     2581 gggacggtgg cccagaacgc atggccgggg gcaccgctgg cgatgtagaa gtcgagcagg
     2641 cgctcgaggg tggcggcggc gcggcgggcc gggaccttgc cgagcaggcg gccgaagtcg
     2701 gcgccgtcgg cgcggatccc gccgccgacg tagaccaggt actgcggggc ggggcggccg
     2761 gcgaccttgc gcatgccgcc ctggaagccg atgccggcga tgtagtgctg gccgcagccg
     2821 ttggggcagc ccgagacctt gacgtcgagg ccgcgcgcac cggcgatcag gtcggggcgg
     2881 cgatcgagca gctcggtgag ctgggcgccc aggccgcgcg acgcggtgac cgcgatcttg
     2941 cagctcgacg cgcccgggca gctgacgacg tcggcgacgg tgttggcgtc cttgcgcgcg
     3001 aggccgaccc ggacgagctc gcggtgcagc gccggcagcc gggcgtcggg cacgaaccgg
     3061 aggacgaggt tctgctcgtt cgtggtgcgc agctcgccct cgccgtaccc gacgaccagg
     3121 gtcgcgagcg cgcggagctg cgcggcggtg acgtcgccga gcacgaggcg gatcacgacc
     3181 gaggagaacc cgtgctgctt ctggggccgg acgttgtgcg cggcccactc ggcgtagccg
     3241 ggctcgacgc tgccgacctg ggcgagcggg gcgcggcgcg gcgggcgggc accttgccga
     3301 gcaggcggcc gaagtcggcg ccgtcggcgc ggatcccgcc gccgacgtag accaggtact
     3361 gcggggcggg gcggccggtg accttgcgca tgccgccctg gaagccgatg ccggcgatgt
     3421 agtgctggcc gcagccgttg gggcagcccg agaccttgac gtcgaggccg cgcgccgccg
     3481 cgatcaggtc gggccggcgg tcgagcagct cggtgagctg ggcgcccagg ccgcgcgacg
     3541 cggtgaccgc gatcttgcag ctcgacgcgc ccgggcagct gaccacgtcg gcgacggtgt
     3601 tggcgtcctt gcgcgccagg ccgacctgga cgagctcgcg gtgcagcgcg ggcagccgcg
     3661 tcgtcggcac ccaacgcagc gcgatgttct gctcgttcgt catgcggacc tcgccctcgc
     3721 cgaactgcac gcacagcgtg gcgagcgcac ggaactgcgc ggcggtgaga tcgccgagcg
     3781 gca
//
LOCUS       DASVOR010001474         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_73304,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001474 DASVOR010000000
VERSION     DASVOR010001474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73304"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF516_27895"
     CDS             <1..534
                     /locus_tag="VF516_27895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8111597.1"
                     /translation="RVLNLFSYTGALSVTCARAGAAAVTSVDTSAGVQAWAAGNFARS
                     GLVDARRWRFETGDAVRFLARAARDKERYDAVLIDPLTFSTARGAPWTLDRDYPDLIA
                     RAAAVIPPSGLLWLAANTHGLGHEPGSLARLAHKGLRAAGRSAAIVEQGGLPPEYPTL
                     AAQPHDRYLQVCVLRLA"
     gene            562..1095
                     /locus_tag="VF516_27900"
     CDS             562..1095
                     /locus_tag="VF516_27900"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX8111598.1"
                     /translation="MTISLHELAIDTFVHMLGDLTHVLDKAAKHAQARNFPADDLASF
                     RLAPDMFPLSTQVYLACHHAKDGPARLQGQEPPALERGLRETFEQSGARIRTTLDHLR
                     GIPKGALDAAAQRKITIEINPERVFDLTGFQLIRDWTLPHFYFHVVTAYDILRAAGVE
                     IGKRDYVPHVAAYLRKP"
     gene            1173..>1766
                     /locus_tag="VF516_27905"
     CDS             1173..>1766
                     /locus_tag="VF516_27905"
                     /inference="COORDINATES: protein motif:HMM:NF014626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HEX8111599.1"
                     /translation="MCGRYTLTTHEQIAEDLQASLDVSVTADPWWKPRFNVAPTQPAP
                     VVVAHDGARSIELMRWGLVPFWAEPGKKLPLMINARVESLMAKQMFRDALERKRCLVP
                     ANGFFEWVRSGDKQPARPFYFHPPGPHLIAFAGLWARHRDPRGDELHSFTIITTRANP
                     LVRPIHDRMPIALPPDDHAAWLSPDLDAEAARALLGHP"
BASE COUNT          253 a          704 c          574 g          235 t
ORIGIN      
        1 cgcgtcctca atctgttctc gtacaccggc gcgctcagcg tgacctgcgc gcgcgccggg
       61 gccgccgccg tgacctcggt cgacacctcg gccggcgtcc aggcctgggc ggccggcaac
      121 ttcgcgcgca gcggcctggt cgacgccaga cgctggcggt tcgagaccgg cgacgcggtc
      181 cggttcctcg cgcgcgccgc ccgcgacaag gagcgctacg acgccgtgct gatcgatccg
      241 ctgacgttct cgaccgcgcg cggcgcgccg tggacgctcg accgcgacta ccccgacctg
      301 atcgccaggg ccgcggcggt gatcccgccc agcggcctct tgtggctcgc cgccaataca
      361 catggactcg ggcacgagcc cggctccctc gcccggctcg cccacaaggg cctgcgcgcg
      421 gccggccgca gcgcggcgat cgtcgagcaa ggcggcctcc ctccggagta cccgacgctc
      481 gccgcccagc cccacgaccg ctacctgcag gtctgcgtgt tgcgcctggc gtagcgccgg
      541 cgatcgccgt agaagagggc catgaccatc tcgctccacg agctcgccat cgacaccttc
      601 gtgcacatgc tgggcgacct gacccacgtg ctcgacaagg ccgccaagca cgcccaggcc
      661 cgcaacttcc cggccgacga cctggccagc ttccggctgg cacccgacat gtttccgcta
      721 tcgacccagg tctacctcgc ctgtcaccac gccaaggacg gcccggcgcg gctgcagggc
      781 caggagccgc cggcgctcga gcgcggcctg cgcgagacgt tcgagcagag cggcgcgcgg
      841 atcaggacca cgctcgacca cctgcgcggc atcccgaagg gcgcgctcga cgccgccgcg
      901 cagcgcaaga tcacgatcga gatcaacccg gagcgcgtgt tcgacctgac cgggttccag
      961 ctgatccgcg actggacgct gccgcacttc tacttccacg tcgtcaccgc ctacgacatc
     1021 ctccgcgccg cgggcgtcga gatcggcaag cgcgactacg tgccccacgt cgccgcgtac
     1081 ctgcgcaagc cgtgaccgcc ggaatttcgg cagcttgacc ggcaccggcc gcgcgcgccg
     1141 atcggcccgg aacaggggat actgtggtca ggatgtgcgg ccgctacacc ctgaccaccc
     1201 acgagcagat cgccgaggac ctccaggcct cgctcgacgt ctcggtgacc gccgacccgt
     1261 ggtggaagcc gcggttcaac gtggcgccga cccagccggc gccggtggtc gtcgcgcacg
     1321 acggcgcgcg gtcgatcgag ctgatgcgct ggggcctcgt cccgttctgg gccgagcccg
     1381 gcaagaagct gccgctcatg atcaacgcgc gggtcgagtc gctgatggcc aagcagatgt
     1441 tccgcgacgc gctcgagcgc aagcgctgcc tggtgccggc caacggcttc ttcgagtggg
     1501 tgcgcagcgg cgacaagcag cccgcccgcc cgttctactt ccacccgccc ggcccgcacc
     1561 tgatcgcgtt cgccgggctg tgggcgcgcc atcgggaccc gcgcggcgac gagctccaca
     1621 gcttcacgat catcacgacc cgcgccaacc cgctggtccg gccgatccac gatcgcatgc
     1681 cgatcgcgct gccgcccgac gaccacgccg cgtggctgtc gcccgacctc gacgccgagg
     1741 ccgcccgcgc cctgctcggc cacccg
//
LOCUS       DASVOR010001475         2073 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_482773,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001475 DASVOR010000000
VERSION     DASVOR010001475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482773"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            223..1266
                     /locus_tag="VF516_27910"
     CDS             223..1266
                     /locus_tag="VF516_27910"
                     /inference="COORDINATES: protein motif:HMM:NF024813.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8111600.1"
                     /translation="MSFIKGGVAELAIARPDSAKDQWVYKHPDQTIPMKAQLTVDSDE
                     VALFFKDGRFVGSFSAGRHTLDASNIPFLGQLIDKFTGGNVFLCEVFFVTTRELPSIK
                     FGTSIGDVQDPQTRLRIRLMVYGEFSARVIDPTKLVIGLVGQRATSNDAFLGWFKSQV
                     QKVMKDGVAELIVKQSWPLMKVTSGAYTDEIEQATLQGVRAHIEPYGVEIIRFGDFTI
                     SMDQADKERLDKLVDRMAYVEGMGSTTGGMAAYQQLAQAEMMMGAAEGMKKGGDAGGA
                     FQGAGIGLGFAMAGNMAGTMGNASAAQPQRGPAESKDQIMAMLKQLGELKAAGVLTDA
                     EFDAKKKELLAKL"
     gene            1282..1998
                     /locus_tag="VF516_27915"
     CDS             1282..1998
                     /locus_tag="VF516_27915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8111601.1"
                     /translation="MVQLDNRAYYDDFAGWYENERHLPYHRMLDDLEVELVERYAAGK
                     AVLEVGCGTGLILHRTARFARRAAGIDLSGAMLARAHQRGLRVVQASATDLPIATASV
                     DVAYSFKVLAHIPDIEGALREMARVVRPGGWVLAEFYNARSLRRLVKAVKPPTAVSDT
                     THDGHVFTRYDDAAAIRSYLPADLEWVATRGIRVITPIARVLEVPVLGAAVRWAEHRL
                     ADLPGARDVGGFLVACCRRR"
BASE COUNT          340 a          720 c          719 g          294 t
ORIGIN      
        1 ggacccgggt ccggctcgcc gcgcggcccc tatcggaccc gccatccccg ccgtccggcc
       61 caccgacaag ctgagggaaa cccggagctt ggcgcgaaaa cccgcggccc gcccggagcg
      121 ttccatgtca tcttgaggaa gctcgcaagg gtgcttacgg tatgcaccat cacgcaggcc
      181 cgggggctcg taccggctgc aggaggcttc acgatgggaa tcatgagttt catcaagggt
      241 ggcgtcgccg agctggcgat cgcgcggccg gactcggcga aggaccagtg ggtctacaag
      301 catccggatc agacgatccc gatgaaggcc cagctcacgg tcgacagcga cgaggtcgcg
      361 ctgttcttca aggacggcag gttcgtcggc agcttctccg ccgggcgcca caccctcgat
      421 gcctccaaca tcccgttcct cggccagctg atcgacaagt tcaccggcgg caacgtcttc
      481 ctctgtgaag tcttcttcgt caccacccgc gagctgccgt cgatcaagtt cggcacctcg
      541 atcggcgacg tccaggatcc gcagacccgg ctgcggatcc ggctcatggt ctacggcgag
      601 ttctccgccc gcgtgatcga tccgaccaag ctcgtgatcg gcctggtcgg ccagcgcgcg
      661 accagcaacg acgcgttcct gggctggttc aagagccagg tccagaaggt gatgaaggac
      721 ggcgtcgccg agctgatcgt caagcagagc tggccgctca tgaaggtgac gtcgggcgcc
      781 tacaccgacg agatcgagca ggccacgctg cagggcgtgc gcgcccacat cgagccctac
      841 ggcgtcgaga tcatccggtt cggcgacttc acgatctcga tggaccaggc cgacaaggag
      901 cggctcgaca agctggtcga ccggatggcg tacgtcgagg gcatgggctc gacgaccggc
      961 ggcatggcgg cctaccagca gctggcgcag gccgagatga tgatgggcgc ggccgagggc
     1021 atgaagaagg gcggcgacgc cggcggcgcg ttccagggcg ccggcatcgg cctgggcttc
     1081 gcgatggcgg gcaacatggc gggcacgatg ggcaacgcca gcgcggcgca gccccagcgc
     1141 ggcccggccg agagcaagga ccagatcatg gcgatgctca agcagctcgg cgagctcaag
     1201 gccgccggcg tcctgaccga cgccgagttc gacgcgaaga agaaggaact gctggccaag
     1261 ctgtagtaga acgcgcaggg catggtccag ctcgacaacc gcgcgtacta cgacgacttc
     1321 gccggctggt acgagaacga gcgccacctg ccctaccacc ggatgctcga cgatctggag
     1381 gtcgagctgg tcgagcgcta cgccgccggc aaggcggtgc tcgaggtcgg ctgcggcacc
     1441 gggctgatcc tgcaccgcac ggcgcggttc gcgcgccgcg ccgccggcat cgacctgtcc
     1501 ggcgccatgc tcgcccgggc ccaccagcgc ggcctgcgcg tggtccaggc gtcggccacc
     1561 gacctgccga tcgcgaccgc gtcggtcgac gtcgcctact cgttcaaggt gctcgcccac
     1621 atccccgaca tcgagggcgc gctgcgcgag atggcgcggg tcgtccgccc cggcggctgg
     1681 gtgctcgccg agttctacaa cgcgcgctcg ctgcgccggc tggtcaaggc ggtcaagccg
     1741 ccgacggcgg tgtccgacac cacccacgac gggcacgtgt tcacccgcta cgacgacgcc
     1801 gccgcgatcc gcagctacct gcccgccgac ctcgaatggg tcgcgacccg cggcatccgc
     1861 gtgatcacgc cgatcgcccg ggtgctggag gtgccggtgc tgggcgcggc ggtgcgctgg
     1921 gccgagcacc ggctcgccga tctgccgggc gcgcgcgacg tcggcggatt cctggtcgcg
     1981 tgctgccggc ggcggtagcg aagtacagat cccgcaagtt ggcgccccac agcgtccgca
     2041 gggccgcgtg atgcatcgtg atcctgctgg acg
//
LOCUS       DASVOR010001476         4346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_336215,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001476 DASVOR010000000
VERSION     DASVOR010001476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336215"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VF516_27920"
     CDS             <1..1391
                     /locus_tag="VF516_27920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111602.1"
                     /translation="PPDATWYCARPDGTRHGPFVTLFPDGSPAIAGSYKDGALDGPWQ
                     RHRATGAVIESGSYAAGQKTGTWRMFGPTGALLGEYEMAAGTGTETWWLDDGTLYRER
                     ALRAGAPSGAERIYAADGTPLVAAQWAGGKLDGPHTVGTKATLRLEETFAKGLRRGSR
                     QIALFQTPLVDESYDRRGKLDGDYTIWRSKKVMRAHGRYVHGKRDGVWTWNDRDGNPE
                     RVGSYVDGKRDGQWTEWFDGKNVFTGNYTLGKPDGEFVYNDHSETVLGRFEIKGGTGT
                     MLTFWPNKKVASRQHLFRGVADGLYQELTNRGKVVVEGHYRNDLRHGAWKEWTADGVP
                     TLEQTWKRGKLDGVVRKYVDGKLATEATYKDGKATGRYVEYRGGKPAVTGQFADDKKT
                     GVWTEYDADGRVTLTATYKDGVLDGAWRQLVGGVVLEGTLTRGRRTGTWTQTDKAGEV
                     RQQSYAAGTSSR"
     gene            1505..2257
                     /locus_tag="VF516_27925"
     CDS             1505..2257
                     /locus_tag="VF516_27925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111603.1"
                     /translation="MSAVNRDIKTQAALIALARTRRMQRALAHAYLHRDAPLPTLHSS
                     LGYMTTTARVTDEAAAIGHRMTVLGIARLEGVAYVVEEIDDDSAPVVYRLYLRGARQG
                     HLVPIHAWYEHGDDDDEIRTRIAALAPLLEPARPTAPEAWMLSTRIIQRRALRVSTCS
                     SLPIRKYALQLVVEPVRGHGPSGRSTVTAFLSPHARLTEVWSLARTPDAPCDRAIARV
                     TFTGIPSGPGLNKDTVVLLTTGSFDDAPDSPD"
     gene            2346..2837
                     /locus_tag="VF516_27930"
     CDS             2346..2837
                     /locus_tag="VF516_27930"
                     /inference="COORDINATES: protein motif:HMM:NF024515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX8111604.1"
                     /translation="MRLLVLSLVLAPVLAPALLAAGCHGGQTGPDADEALACMTSGRG
                     DTYVVGLEHKGVNTVYDFKLMSAMPAPPGRNLNTWVIQISWEATGAPVTKASLTVSPF
                     MPDHQHGPGAYTPQVMELATPGQYQISDINTWMPGYWEITIDAKTPDNIEDTVVYKFC
                     IPA"
     gene            complement(2863..>4346)
                     /locus_tag="VF516_27935"
     CDS             complement(2863..>4346)
                     /locus_tag="VF516_27935"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HEX8111605.1"
                     /translation="RARRLGPGARGDAVTRVALTGAGVAFALGLAGCGGDGPARSVAE
                     LEDPQTCNECHPRHYTQWSASMHAYASDDPVFLAMNRRGQRETGGALGTFCVKCHAPM
                     AVELGLTTGADFDPAKLPAAARGVTCYFCHNVESVQDSHNNGLALAHDQTMRGGLPGP
                     ASSPAHHSAYDRLMDSTVNNSEMCGSCHDVVTPRGVKLERTYEEWQTTFFAQPDPQHH
                     LTCGACHMPSSDGVIAEAPGLSVKSHNGGFHEHLWPGIDQALTPLPGAESLAAGIKRD
                     LDPAVAIIGPTVFATNLNYGGICVDPPGVLSVHRDSIGTAHGWPTGAAQDRRAWLEVV
                     AYDAANQVVFHSGTLDDGMGTPLPDGMDPEQLADPDLFGLWDRASRDDASPAHMFWDI
                     AQLDSRQLLRPPVTLDKNSPLFDHSSTKMFPVQGVIGSIDHITARIRIRPLSYALLAD
                     LVGSGDLAAGAVPRLPTLDILGTQHTWKADPATHQPGTNCNLH"
BASE COUNT          678 a         1558 c         1542 g          568 t
ORIGIN      
        1 cgccgcccga cgcgacgtgg tactgcgcgc gtcccgacgg cacgcggcac ggcccgttcg
       61 tcacgctgtt ccccgacggc tcgcccgcga tcgccggcag ctacaaggac ggcgcgctcg
      121 acgggccgtg gcagcgccac cgcgcgaccg gcgcggtgat cgagagcggg agctacgcgg
      181 ccggccagaa gaccgggacc tggcggatgt tcggcccgac cggcgcgctg ctcggcgagt
      241 acgagatggc cgccggcacc ggcaccgaga cgtggtggct cgacgacggc acgctgtacc
      301 gcgagcgcgc gctcagggcc ggcgcaccga gcggcgccga gcggatctac gcggccgacg
      361 gcacgccgct ggtcgcggcg cagtgggccg gcggcaagct cgacgggccg cacaccgtcg
      421 gcaccaaggc cacgctgcgg ctcgaggaga cgttcgccaa ggggctgcgg cgcggcagcc
      481 ggcagatcgc gctgttccag accccgctgg tcgacgagtc gtacgaccgc cgcggcaagc
      541 tcgacggcga ctacacgatc tggcgcagca agaaggtcat gcgcgcccac ggccgctacg
      601 tgcacggcaa gcgcgacggg gtgtggacgt ggaacgaccg cgacggcaac cccgagcgcg
      661 tcggcagcta cgtcgacggc aagcgcgacg gacagtggac cgagtggttc gacggcaaga
      721 acgtgttcac cggcaactac accctgggca agcccgacgg cgagttcgtc tacaacgacc
      781 acagcgagac cgtgctcggc cggttcgaga tcaagggtgg caccggcacg atgctgacgt
      841 tctggcccaa caagaaggtc gcgtcgcgcc agcacctgtt ccggggggtc gccgacgggc
      901 tgtaccagga gctgaccaac cgcggcaagg tcgtggtcga ggggcactac cgcaacgacc
      961 tgcgccacgg cgcatggaag gagtggacgg ccgacggcgt gccgacgctg gagcagacct
     1021 ggaagcgcgg caagctcgac ggcgtcgtcc gcaagtacgt cgacggcaag ctcgcgaccg
     1081 aggcgaccta caaggacggc aaggccaccg gcaggtacgt cgagtaccgg ggcggcaagc
     1141 cggcggtgac cggccagttc gcggacgaca agaagaccgg cgtgtggacc gagtacgacg
     1201 ccgacggccg cgtcacgttg accgcgacct acaaggacgg cgtgctcgac ggcgcgtggc
     1261 gccagctggt cggcggcgtc gtgctggagg gcacgctgac gcgcggccgc cgcaccggga
     1321 cctggaccca gaccgacaag gccggcgagg tgcggcagca gagctatgcc gccggaacct
     1381 catcgcgcta gcggaagcgt ggcgcggcgc actgcgaaaa gccctgaccg cgctcggggg
     1441 caaccgccgc cgtaacaaag agattccgga tgcaaccggt ggccggcttg cacgttctca
     1501 ggacatgagc gccgtgaatc gcgacatcaa gacccaggcc gcgctgatcg cgctggcgcg
     1561 cacgcgccgc atgcagcgcg ccctcgccca cgcctacctg caccgcgacg ctccgctgcc
     1621 gaccctgcac agctcgctcg gctacatgac caccaccgcg cgcgtcaccg acgaagccgc
     1681 cgcgatcggc caccggatga ccgtgctcgg catcgcccgg ctggagggcg tggcgtacgt
     1741 cgtcgaggag atcgacgacg acagcgcgcc ggtcgtctac cggctctacc tgcgcggcgc
     1801 gcgccagggc cacctggtgc cgatccatgc ctggtacgag cacggcgatg acgacgacga
     1861 gatccgcacg cggatcgcgg cgctggcccc gctgctcgag ccggcgcggc cgacggcgcc
     1921 cgaggcgtgg atgctgtcga cgcggatcat ccagcgccgc gcgctgcggg tctctacctg
     1981 cagcagcctg ccgatccgca agtacgcgct ccagctcgtc gtcgagccgg tccgcgggca
     2041 cggcccgagc ggccggtcga ccgtgaccgc gttcctgtcg ccgcacgccc gcctcaccga
     2101 ggtctggtcg ctcgcccgca cccccgacgc gccctgcgat cgcgcgatcg cgcgcgtgac
     2161 gttcaccggc atcccgagcg gccccgggct caacaaggac actgtggtgc tgctcacgac
     2221 cggatcgttc gacgacgcgc ccgattctcc cgattgatcg ggatgttagg caacgtgatg
     2281 gaacggaaca tggggccttc cggcgggcct ggtaccttgt gcccgcaccg tctcgtagga
     2341 gccccatgcg cctgctcgtg ctctccctgg ttctggcccc ggttctggct ccggctctgc
     2401 tcgcggccgg ctgtcacggc ggccagaccg ggcccgatgc cgacgaggcg ctcgcgtgca
     2461 tgaccagcgg gcgcggcgac acctacgtcg tcgggctcga gcacaagggc gtgaacacgg
     2521 tctacgactt caagctgatg tcggcgatgc cggccccgcc cggccgcaac ctcaacacct
     2581 gggtgatcca gatcagctgg gaggccaccg gcgcgccggt gaccaaggcg tcgctgaccg
     2641 tcagcccgtt catgccggat caccagcacg gccccggcgc ctacacaccg caggtcatgg
     2701 agctggccac gccggggcaa taccagatct cggacatcaa cacctggatg ccgggctact
     2761 gggagatcac gatcgacgcg aagacgcccg acaacatcga ggacaccgtc gtctacaagt
     2821 tctgcatccc ggcctgacgc cgcggcggcc cggcgcaccg ggtcagtgca ggttgcagtt
     2881 ggtgcccggc tggtgggtcg ccgggtcggc cttccacgtg tgctgggtgc cgaggatgtc
     2941 gagcgtcggc agccggggca cggcgccggc cgcgaggtcg cccgagccca ccaggtcggc
     3001 gagcagcgcg tagctcagcg gccggatccg gatccgcgcc gtgatgtggt cgatgctgcc
     3061 gatgacgccc tggaccggga acatcttggt cgacgagtgg tcgaacagcg gcgagttctt
     3121 gtcgagcgtg accggcggcc gcagcagctg gcggctgtcg agctgtgcga tgtcccagaa
     3181 catgtgcgcc ggcgaggcgt cgtccctgga cgcgcgatcc cacagcccga acaggtccgg
     3241 atcggcgagc tgctcgggat ccatgccgtc gggcagcggc gtgcccatgc catcatcgag
     3301 cgtaccgctg tggaacacca cctggttcgc ggcgtcgtac gcgacgacct ccagccacgc
     3361 gcggcggtcc tgggcggcgc cggtcggcca gccgtgcgcg gtgccgatcg agtccctgtg
     3421 caccgacagc acgcccggcg gatcgacgca gatcccgccg taattcaggt tggtggcgaa
     3481 caccgtcggg ccgatgatcg cgaccgccgg atcgaggtcg cgcttgatcc cggccgcgag
     3541 cgactccgcg ccgggcagcg gggtcagcgc ctgatcgatc cccggccaca ggtgctcgtg
     3601 gaacccgccg ttgtgcgact tgacgctcag ccccggcgcc tcggcgatca cgccgtccga
     3661 cgacggcatg tgacacgcgc cgcaggtcag gtggtgctgc ggatcgggct gcgcgaagaa
     3721 cgtggtctgc cactcctcgt aggtgcgctc cagcttgacc ccgcgcgggg tcaccacgtc
     3781 gtggcacgac ccgcacatct cggagttgtt caccgtcgag tccatcagcc ggtcatacgc
     3841 cgagtggtgc gccggcgagc tcgccggccc gggcagcccg ccgcgcatcg tctgatcgtg
     3901 ggcgagcgcc aggccgttgt tgtggctgtc ctggaccgac tcgacgttgt ggcagaagta
     3961 gcaggtcacg ccgcgcgccg ccgccggcag cttcgccgga tcgaagtcgg cgccggtggt
     4021 caacccgagc tcgaccgcca tcggcgcgtg gcacttgacg cagaacgtgc cgagcgcgcc
     4081 gccggtctcg cgctggccgc gccggttcat cgcgaggaac accggatcgt cggacgcgta
     4141 cgcgtgcatc gacgccgacc actgcgtgta gtgcctgggg tggcactcgt tgcaggtctg
     4201 cggatcctcg agctcggcga ccgacctcgc cggaccgtcg ccgccgcacc cggcgagccc
     4261 gagcgcaaag gccacgccgg ctcctgtcaa cgccacgcgc gtcacggcgt ccccccgggc
     4321 gcctggcccg agccggcgag cgcgcc
//
LOCUS       DASVOR010001477         2585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_365803,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001477 DASVOR010000000
VERSION     DASVOR010001477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2585
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365803"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2585
                     /locus_tag="VF516_27940"
     CDS             <1..>2585
                     /locus_tag="VF516_27940"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8111606.1"
                     /translation="AVTPAAPATPPNPEDVPLPLWPEVKHGKLANGLTYYVLKHGKPE
                     KRAFLWLAVNAGSVQENDDQRGLAHFDEHMAFNGTRRFPKAEIVNYLEKIGMRFGADL
                     NARTTFDDTVYELEVPTDKKAFLDKGLDILHDWAGDVAYDPVEVDKERGVVMEEWRLG
                     RGAQQRLFDKEAPVLFKGSRYAVRLPIGLPEVIRKAPRDKLHQFYKDWYRPDLMAVIA
                     VGDFDDPGAIEKAIQARFADLQNPPGERPRISAGVPVADGTRVSIETDRELPETVLTI
                     YNLMPHRPEASAHDFRRILVEQIYQRILNDRLAVIARRPDAPFAQAAVGIESPTREVD
                     VFAREAQAKAGQVEAALDGLLTEVLRVEQHGVTQSELDRARAVLIRFYEQIEAEDATS
                     DSRNFTEEFTRNFFEGEFMIGRKAESALAREILPTLTLAELNQLGKSFGGAASRVILI
                     DGPEAHKAALPTRERVLALIDEVSRRTIDPWEDKAVTAKLMEHAPRPGKIVKEARRDA
                     IGVTEWTLSNGARVIIKPTDYQADEVSLLGTSPGGLAMASDKDFANARFADDIAAISG
                     FGDHDAEELGKLLAGKHATAGAEIGDTTESVRAAASVRDLETMFQLVHLEMTAPRKDD
                     RTIAAWRQNLKEQLINRLRDPEVQYSIQSTKLLYHDNPRRMPATPGDVDKLDPAKALA
                     FYQQRFGDATDFTFVIVGAFDPATLRPLVETYLASLPAHGRKEKQKDLGIRKAPGVVK
                     HAWMLGQEPKAHVEILFHGDEPWSRDKDRDLFILGQVLEIKLREVLREDKGGVYGVGA
                     GGRLIRAPHPERDFSISFGCDPLRITELINATFDEIAALQKTGIGDDYLEKVKQTFTR
                     EREVQ"
BASE COUNT          448 a          920 c          893 g          324 t
ORIGIN      
        1 ggccgtcacc ccggccgcgc cggccacgcc gcccaacccc gaggacgttc cgctgccgct
       61 gtggccggag gtcaagcacg gcaagctggc caatggcctg acctactacg tgctcaagca
      121 cggcaagccc gagaagcggg cgtttttgtg gctggcggtc aacgccggct cggtgcagga
      181 gaacgacgac cagcgcggcc tggcgcactt cgacgagcac atggcgttca acggcacccg
      241 ccggttcccc aaggccgaga tcgtcaacta cctcgagaag atcggcatgc ggttcggcgc
      301 cgatctcaac gcgcggacca cgttcgacga cacggtctac gagctcgagg tcccgaccga
      361 caagaaggcg ttcctcgaca agggcctcga catcctgcac gactgggccg gcgacgtcgc
      421 ctacgacccg gtcgaggtcg acaaggagcg cggcgtggtg atggaggagt ggcggctcgg
      481 ccgcggcgcg cagcagcggc tgttcgacaa ggaggcgccc gtgctgttca agggctcgcg
      541 ctacgcggtg cggctgccga tcggcctgcc cgaggtgatc cggaaggcgc cgcgcgacaa
      601 gctgcaccag ttctacaagg actggtaccg gcccgacctg atggcggtga tcgcggtcgg
      661 cgacttcgac gacccgggcg cgatcgagaa ggcgatccag gcccggttcg ccgatctgca
      721 gaacccgccg ggcgagcggc cgcggatctc cgccggggtg ccggtcgccg acggcacgcg
      781 ggtgtcgatc gagaccgacc gcgagctgcc cgagaccgtg ctgacgatct acaacctcat
      841 gccgcaccgc cccgaggcgt cggcccacga cttccggcgg atcctcgtcg agcagatcta
      901 ccagcggatc ctcaacgacc ggctcgccgt gatcgcccgg cgcccggacg ccccgttcgc
      961 ccaggccgcg gtcgggatcg agagcccgac ccgcgaggtc gacgtgttcg cgcgcgaggc
     1021 ccaggccaag gccggccagg tcgaggccgc gctcgacggc ctgctcaccg aggtgctgcg
     1081 cgtcgagcag cacggcgtga cccagagcga gctcgaccgg gcgcgcgcgg tcctgatccg
     1141 gttctacgag cagatcgagg ccgaggacgc gacgtcggac agccgcaact tcaccgagga
     1201 gttcacccgc aacttcttcg agggcgagtt catgatcggc cgcaaggccg agtccgcgct
     1261 cgcccgcgag atcctgccga cgctcacgct ggccgagctg aaccagctcg gcaagtcgtt
     1321 cggcggcgcc gccagccggg tgatcctgat cgacggcccc gaggcccaca aggcagcgct
     1381 gccgacccgc gagcgcgtgc tggcgctgat cgacgaggtc agccgccgga cgatcgaccc
     1441 gtgggaggac aaggcggtga ccgccaagct gatggagcac gcgcccaggc cgggcaagat
     1501 cgtcaaggag gcccggcgcg acgcgatcgg ggtcaccgag tggacgctgt ccaacggcgc
     1561 gcgcgtgatc atcaagccga ccgactacca ggccgacgag gtgtcgctgc tcggcacctc
     1621 gccgggcggg ctcgcgatgg cgagcgacaa ggacttcgcg aacgcgcggt tcgccgacga
     1681 catcgccgcg atcagcgggt tcggcgatca cgacgccgag gagctcggca agctgctcgc
     1741 cggcaagcac gcgaccgccg gcgccgagat cggcgacacc accgagtcgg tccgcgccgc
     1801 cgcctcggtg cgcgacctcg agaccatgtt ccagctcgtc cacctcgaga tgaccgcgcc
     1861 gcgcaaggac gaccgcacga tcgcggcgtg gcgccagaac ctcaaggagc agctgatcaa
     1921 ccggctgcgc gatcccgagg tccagtactc gatccagtcg accaagctgc tctaccacga
     1981 caacccgcgg cggatgccgg cgacgccggg cgacgtcgac aagctcgacc ccgccaaggc
     2041 gctggcgttc taccagcagc ggttcggcga cgccaccgac ttcacgttcg tgatcgtcgg
     2101 cgcgttcgac ccggcgacgc tccgcccgct ggtcgagacc tacctggcga gcctgccggc
     2161 gcacggccgc aaggagaagc agaaagatct cgggatccgc aaggcgcccg gcgtggtcaa
     2221 gcacgcctgg atgctcggcc aggagcccaa ggcgcacgtc gagatcctgt tccacggcga
     2281 cgagccgtgg agccgcgaca aggaccgcga cctgttcatc ctcggccagg tcctcgagat
     2341 caagctgcgc gaggtgctgc gcgaggacaa gggcggggtc tacggcgtcg gggccggcgg
     2401 ccggctgatc cgcgcgcccc accccgagcg cgacttctcg atctcgttcg gctgcgatcc
     2461 gctgcggatc accgagctga tcaacgccac gttcgacgag atcgccgcgc tgcagaagac
     2521 cgggatcggc gacgactacc tcgagaaggt caagcagacg ttcacccgcg agcgcgaggt
     2581 ccagc
//
LOCUS       DASVOR010001478         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_423570,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001478 DASVOR010000000
VERSION     DASVOR010001478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423570"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF516_27945"
     CDS             complement(<1..293)
                     /locus_tag="VF516_27945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111607.1"
                     /translation="MQACSGCGTPAVEAGTFCPACGASMASSGARTDAGAGTRPSTEL
                     AATQPGAPASSAGSLVGRTIGDFVIEGVIGGGSFGTVYRGRQLGLDRPVAIKVP"
     gene            complement(1171..1827)
                     /locus_tag="VF516_27950"
     CDS             complement(1171..1827)
                     /locus_tag="VF516_27950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8111608.1"
                     /translation="MTNAQERKAQERQARRRRIQEAARTVFTERGYAGASIELIARAA
                     QLSVGAIYLYFRSKEDLYVSLIEDTLTLFDVEMGQLRERHDVSHRLRETWNTLVRWAE
                     KDPEGPRILRLLAQPAIRPQLSDEVVAAVSAGIHRVQEHLGACVADGIHAGIYRQANA
                     REIADLAWSVLLGSLGAAEMQANLGLGTEPLTSRTDRALALIESALAPTVAGSLRAVA
                     "
     gene            2282..>3154
                     /locus_tag="VF516_27955"
     CDS             2282..>3154
                     /locus_tag="VF516_27955"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8111609.1"
                     /translation="MTITTGRPAVPSSGRPSLSLAIVIDRSGSMSGAPIEHAKAAALA
                     MIRQLDGGDAFSVVSYSSSARTVLAIQRATEANKEAARAAIETIDAGGGTCISCGLEA
                     GAAEVARSPIAAGLRRMLLISDGQANAGVYNRDELAGLAAHTAARGVSISTAGVGLDF
                     DEATMRRLAEVGRGNYYFVEDTVALSAMFGHELATTSQTVASDIFLSVTSRPGIRVED
                     AYGYPLTRLADGVVRVPVADLRAGEVRKIVLRIRVAAGHTGALDLADLRLDWRRVGGG
                     GSGAARAVASVDVVD"
BASE COUNT          494 a         1083 c         1064 g          513 t
ORIGIN      
        1 gggaccttga tcgcgaccgg gcgatcgagc ccgagctggc ggccgcggta caccgtgccg
       61 aacgagccgc cgccgatcac gccctcgatc acgaagtcgc cgatcgtgcg gccgaccagg
      121 ctgccggccg acgacgcggg cgcgccgggc tgggtcgcgg ccagctctgt gcttggtctg
      181 gttcccgcgc cggcatcggt ccgggcgccg gacgacgcca tcgaagcgcc gcacgccggg
      241 cagaacgtcc cggcctccac cgcgggagtc ccacagccgg aacaggcctg catgtccggc
      301 gagcgtagca gcgacgtccc ggcgcgatcg cgaaaccgcc ccggaggcgc gtccggttgc
      361 gcgtccgggg cttcgatcgc agggggcatg tccattcgac tcggggaccg ggcgattggt
      421 tcggccccgg tgggtgcagc agaagtaggc acgcttactg actttccctc aataacagga
      481 atacgccggt tccgatgacg ccgactcgct gtgggctcga tcccgccggg ctggcgcacc
      541 tcgcgggcga tggtcggacc cagctcttct tcgatgtcgc gtaggtcgct cagcgtgtcg
      601 actggacgcg gatcgacagc gggggaaaac cgcctcgccg cggtgtcgaa ccagcaatcg
      661 ggttcaccga tcccgaccgt cgcggcgacc ctcggcatcg gcgaatcgac gtctcctcgc
      721 cggcggcctc gccgcgccgt acgccggcct tcaccgccac gccgacgaca gcccgcagcg
      781 ttatgcgtca gtcagcctcg ccggctccgc cttcatctat atgtggcgca ggacacccgg
      841 actgactacc tcgcacctcc agcggatcga tcggactaca atataatgta gcgcggagcg
      901 ccggccactg gctcgtttgc caccccggct gccgagatcg gcattcttcg aaggagcgcg
      961 cgctccgagt cggagctgtc attcacgctc cccgcagcga ttcgctccgg tcggacgtcg
     1021 tacgccccgc ggtcacgagg ccggcggctg gcagatcggc gccgcgctgg ggaccgcgat
     1081 cctggcggac gttcaggtgc tgctcgcgga ggcccgggta ccccagctgg cgccgatccg
     1141 gactgcgatt cgcatgcgcg ctgtgaccgg tcaggccacg gcgcgaagcg aaccggccac
     1201 agtgggcgcc agcgcactct cgatcagcgc gagcgcgcga tcggtccgtg acgtgagcgg
     1261 ctcggtgccg agtccgaggt tggcctgcat ctcggcggca cccaggctgc cgagcaatac
     1321 cgaccaggcc agatccgcga tctcccgcgc attggcctgg cgataaatcc cggcatggat
     1381 gccatcggca acacatgcac cgagatgctc ctgaacgcga tggatgcccg ccgacaccgc
     1441 ggcgaccact tcgtcggaca gctgagggcg aatcgctggt tgagccagta gacgcaggat
     1501 gcgcggtcct tctggatctt tttcagccca gcggacgagc gtgttccagg tctctcgcag
     1561 gcggtgcgag acatcgtggc gctcgcgaag ttgccccatc tcgacatcga acaacgtcag
     1621 cgtgtcctcg atcagcgaga cgtagaggtc ttccttggac cgaaagtaca gatagattgc
     1681 gccaacagac agctgcgctg cacgagcgat gagctcgatc gaagcaccgg cgtatccgcg
     1741 ctccgtgaaa accgtgcgag cggcttcctg gatgcgccta cggcgagcct gtcgctcctg
     1801 agccttgcgt tcttgagcgt tcgtcatggc gccgatgtag tatcggtgac gcgttgggtc
     1861 aagcgaagtt ttttagcaag cgatcgccgc tgtcggccca gcctggaaaa ttttatgaaa
     1921 atcatccgct tgtgagatag gtattgactt acgaatctga ctcgttcttc ggttcccaat
     1981 gtggcctggg ccacatctcg gaacggctct gcaacacgac ggatagatcg ccgaagcggt
     2041 gctgaacgtc gaaatgcgaa tggctaccaa tgcgcaaact gcgcatcgaa tccgaacttc
     2101 cctcgtagtc ggcgctgctg ctggcacgat gatcgctgct gcactgatgt cacctcggag
     2161 tgcagtgcat cgtccgtccg acggaacggt gtcttccacg gatggcacgg acggaatttc
     2221 gctatccgca cgactcgcga cccgccggat cctctccggt gaccagccgc agaatgtcgc
     2281 ggtgacgatc accaccggcc gaccggccgt tccgtcgagc gggcgtccgt cgctgtcgct
     2341 cgcgatcgtg atcgaccgct ccggctcgat gagcggcgcg ccgatcgagc acgccaaggc
     2401 cgccgcgctg gcgatgatcc gccagctcga tggcggcgac gcgttctcgg tggtgagcta
     2461 ctcgagctcc gcccggacgg tgctggcgat acagcgcgcg accgaggcga acaaggaggc
     2521 cgcgcgggcg gcgatcgaga cgatcgacgc gggcggcggg acctgcatct cgtgcgggct
     2581 ggaggccggc gccgccgagg tcgcgcgctc gccgatcgcc gccgggctgc gccggatgct
     2641 gctgatctcc gacggtcagg ccaacgcggg cgtgtacaac cgcgacgagc tcgccgggct
     2701 cgccgcgcac accgccgccc gcggggtctc gatctcgacc gccggtgtcg ggctggactt
     2761 cgacgaggcg acgatgcgcc ggctcgccga ggtcggccgc ggcaactact acttcgtcga
     2821 ggacaccgtc gcgctgtcgg cgatgttcgg ccacgagctc gccacgacga gccagaccgt
     2881 ggcgtcggac atcttcctgt ccgtgacctc gcgccccggc atccgcgtcg aggacgccta
     2941 cggctatccg ctgacccgcc tcgcggacgg cgtcgtccgt gtgccggtcg ccgacctgcg
     3001 cgccggcgag gtgcgcaaga tcgtgctgcg gatccgcgtc gcggccggcc acaccggagc
     3061 gctcgacctg gccgatctca ggctcgactg gcgccgcgtg ggcggcggcg ggtccggtgc
     3121 ggcgcgcgcg gtcgcgtcgg tcgacgtggt cgac
//
LOCUS       DASVOR010001479         3360 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_190748,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001479 DASVOR010000000
VERSION     DASVOR010001479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190748"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1613
                     /locus_tag="VF516_27960"
     CDS             <1..1613
                     /locus_tag="VF516_27960"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8111610.1"
                     /translation="APALPAQPPAAGSGAGSGSADRSPDPGPARDRVAPGRAVLSARG
                     VADWPRLSTRINRGVAWAGAAQAIIAIADLVSQVVVGARFVSADDLGLAAGAMAFYTL
                     LDTAADFGVTSGLIQRDDHTPETVSTVFWFNLLVSTGLAVVLLGLGPLYGRFQGHPIV
                     GWLLVAYGGKLVLQNVYAIPFALLRRELRYAEIAKARTAAHLCESVARIAFAALGYTV
                     WCWTLAALIRSAVFGAIMQWRHRFVPKLVFQPRTVLPHVRFGLRTAASQMLYQLYTNL
                     DYAVVLRVFGERANGIYTLAYFIVLEPVKTIANVVIDVAFPAFARLRADRDGLIRQLL
                     QFTRLNLIAVLPFVVLILLVIPEFLRVFYAGGKWTGDELALCGEAARILCIVGVLRGL
                     GFLGPPLLDGIGRPELTLRYMVSATLAVPAMFVAGAVVLGDRLGFLSVAVAWAAGYPL
                     AFAVLAYLVVRSIELPLAPYIRASAGIFGCCGGGALGGLAVSEVLANVSDGVRMVAVG
                     LGALAVTAALLATWQGITPRSIAAALRG"
     gene            1715..2818
                     /locus_tag="VF516_27965"
     CDS             1715..2818
                     /locus_tag="VF516_27965"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8111611.1"
                     /translation="MDVSRGIAGPARSSVVPPGSSRLPRKPRASIVVGSHNRLSCLCE
                     LLERFLAQDFTDFEVIVIEQSTQVTAEQRRQLAELAADPRIRIYPRPPLGGAGARNEG
                     CRLARADIIVAFDDDDLPASTEWLSKLLRNFDDPSCIGVSGRHILENGPNPPYANMER
                     ARRRVLSYSPLMWQRCFPRSDVRSEKIENLMGGNSAVRRSVLERFGLWDAFTRCENEN
                     SLWFRIRNRLEPGEYLVFDPEATMIRRLDVEGGMAKRKIGVLRFGWAMFEYFHKILGY
                     YHPVRFLGLYPVYFVFFFMVICDWVWTESARYRGRYPLALAVLVGFALLQPATWTGWL
                     TRLAIDRIRKGPEKRETEFTPYPDAVAHELAAA"
     gene            complement(2822..>3360)
                     /locus_tag="VF516_27970"
     CDS             complement(2822..>3360)
                     /locus_tag="VF516_27970"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8111612.1"
                     /translation="IGIDRGANLARARASAPALDWRDHDLQGEAALPVSADELAGSVV
                     INADVIEHLPDPHPLLAQLRAAHTHAELILLSTPERHLTRGLRDAGPPGNPAHVQEWT
                     VRELGALLRSAGLAMHSLGLIRAHDHSDQVATIFAAIAQTPERLAEVDRLLIDLEVPP
                     RRGVLDDAAALLTTLRRR"
BASE COUNT          405 a         1183 c         1224 g          548 t
ORIGIN      
        1 ccgcgcccgc gctgcccgcc cagccgccgg cggcgggctc cggggccggc agcggctcgg
       61 cggatcgcag cccggatccg ggaccggccc gtgaccgcgt cgcgcccggc cgcgccgtgc
      121 tatcagcgag gggcgtggca gactggcctc ggctgagcac gagaatcaac cgcggcgtcg
      181 cgtgggcggg cgcggcccag gcgatcatcg cgatcgccga cctggtgtcc caggtcgtcg
      241 tcggcgcccg gttcgtgtcg gccgacgacc tcgggctcgc cgccggcgcg atggcgttct
      301 acaccctgct cgacaccgcg gccgacttcg gcgtgacctc ggggctgatc cagcgcgacg
      361 accacacccc cgagaccgtg tcgaccgtgt tctggttcaa cctgctggtg tcgaccgggc
      421 tggccgtggt gctgctcggg ctcggcccgc tgtacggccg gttccagggc catccgatcg
      481 tcggctggct gctggtcgcg tacggcggca agctggtgct gcagaacgtc tacgcgatcc
      541 cgttcgcgct gctgcgccgc gagctgcgct acgccgagat cgccaaggcg cgcaccgccg
      601 cccacctgtg cgagagcgtc gcgcggatcg cgttcgctgc gctcggctac accgtgtggt
      661 gctggacgct cgcggcgctg atccgcagcg ccgtgttcgg cgcgatcatg cagtggcggc
      721 accgcttcgt gccgaagctg gtgttccagc cccgcaccgt gctgccccac gtccggttcg
      781 gcctgcgcac cgcggccagc cagatgctct accagctcta caccaacctc gactacgccg
      841 tggtcctgcg cgtgttcggc gagcgcgcga acgggatcta cacgctggcc tacttcatcg
      901 tgctcgagcc ggtgaagacg atcgccaacg tcgtgatcga cgtcgcgttc ccggcgttcg
      961 cgcggctgcg cgccgaccgc gacggcctga tccggcagct gctccagttc acccggctca
     1021 acctgatcgc ggtgctgccg ttcgtcgtgc tgatcctgct cgtgatcccc gagttcctgc
     1081 gggtgttcta cgccggcggc aagtggaccg gcgacgagct cgcgctgtgc ggagaggccg
     1141 cgcggatcct gtgcatcgtc ggcgtgctgc gcggcctggg gttcctcggc ccgccgctgc
     1201 tcgacgggat cggccggccc gagctcacgc tgcgctacat ggtgtcggcc acgctggcgg
     1261 tgccggcgat gttcgtcgcc ggcgcggtcg tgctcggcga tcggctcggc ttcctgtcgg
     1321 tcgcggtggc gtgggccgcc ggctacccgc tggcgttcgc ggtgctcgcc tacctcgtgg
     1381 tgcgctcgat cgagctgccg cttgcgccct acatccgggc ctcggcggga atcttcggtt
     1441 gctgcggcgg cggtgcgctc ggcgggctcg cggtgtcgga ggtgcttgcg aatgtttccg
     1501 acggtgtccg gatggtcgcg gtcgggctcg gcgcgctcgc ggtcaccgcc gcgctgctcg
     1561 ccacgtggca agggatcacg ccgcgttcga tcgccgcggc acttcgtggg tgatccgtct
     1621 taaggcccgg cttaaggtgt ggtgtcaaaa actcaacacc acgatggagt ccgtgaagat
     1681 gcagcgcgcg ccgcggttgt ggtagtccgt tcgtatggac gtctctcgcg gcatcgccgg
     1741 gccggcacgc tcgtcggtcg tgccgcccgg atcgtcgagg ctgccgcgca agccgcgcgc
     1801 gtcgatcgtc gtcggctcgc acaaccggct gagctgtctg tgcgagctgc tcgagcggtt
     1861 ccttgcacag gacttcaccg acttcgaggt catcgtgatc gagcagagca cgcaggtcac
     1921 cgccgagcag cggcggcagc tcgccgagct cgcggccgat ccgcggatcc ggatctaccc
     1981 gcggccgccg ctcggcggcg ccggggcgcg caacgagggc tgccggctcg cccgcgccga
     2041 catcatcgtc gcgttcgacg acgacgacct gccggcgtcg accgagtggt tatcgaagct
     2101 gttacgtaac ttcgacgacc cgtcctgcat cggggtcagc ggccggcaca tcctggagaa
     2161 cggccccaac ccgccgtacg ccaacatgga gcgcgcgcgg cgccgggtgc tgagctactc
     2221 gccgctgatg tggcagcgct gcttcccgcg cagcgacgtg cggtccgaga agatcgagaa
     2281 cctgatgggc ggcaactcgg cggtgcgccg cagcgtgctc gagcggttcg ggctgtggga
     2341 cgcgttcacc cggtgcgaga acgagaactc gctgtggttc cggatccgca accggctcga
     2401 gcccggcgag tacctggtgt tcgatcccga ggcgacgatg atccgccggc tcgacgtcga
     2461 gggcgggatg gccaagcgca agatcggggt gctccgcttc ggctgggcga tgttcgagta
     2521 cttccacaag atcctcggct actaccaccc ggtccggttc ctcgggctgt acccggtgta
     2581 cttcgtgttc ttcttcatgg tgatctgcga ctgggtgtgg accgagagcg cgcgctaccg
     2641 cggccggtac ccgctcgcgc tcgccgtgct ggtcggcttc gcgctgctcc agccggcgac
     2701 ctggacgggg tggctgaccc ggctcgcgat cgaccggatc cgcaagggcc ccgagaagcg
     2761 cgagaccgag ttcaccccgt atccggacgc ggtcgcccac gagctggcgg cggcctgacc
     2821 gtcagcgccg ccgcagcgtg gtgagcaggg cggccgcgtc gtcgagcacg ccgcgccggg
     2881 gcgggacctc gaggtcgatc agcagccggt cgacctcggc gagccgctcg ggagtctgcg
     2941 cgatcgccgc gaagatcgtc gcgacctgat cggagtgatc gtgcgcccgg atcaggccga
     3001 gcgagtgcat cgccagcccc gccgagcgca gcagcgcgcc gagctcgcgc accgtccact
     3061 cctggacgtg ggccgggttg ccgggcgggc cggcgtcgcg caggccgcgg gtcaggtggc
     3121 gctccggcgt cgacagcagg atcagctcgg cgtgggtgtg cgccgcgcgc agctgggcga
     3181 gcagcgggtg gggatccggc aggtgctcga tcacgtcggc gttgatcacc acgctgccgg
     3241 cgagctcgtc cgccgacacc ggcagggccg cctcgccctg caggtcgtga tcgcgccagt
     3301 cgagcgccgg cgcgctggcc cgggcgcggg cgaggttggc gccgcggtcg atgccgatcg
//
LOCUS       DASVOR010001480         3639 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_423580,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001480 DASVOR010000000
VERSION     DASVOR010001480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3639
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423580"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VF516_27975"
     CDS             complement(<1..490)
                     /locus_tag="VF516_27975"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8111613.1"
                     /translation="MSGRPSAAGIEQIFDELRDASWTRRVAGLQTVTARLASGNLSQD
                     HETKLIAALCDLAHDPKWEVRKAVTVALAEVRGPEARIQTVLDELARDVNRWVSQAAG
                     RAVRRLRERTGRDTLWTLTAPVQDPILKHIAKRTREIGLRSLTPALIYDIAMEVSEQS
                     YRE"
     gene            complement(490..2433)
                     /locus_tag="VF516_27980"
     CDS             complement(490..2433)
                     /locus_tag="VF516_27980"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8111614.1"
                     /translation="MKSRNENAGSSGPAATAHHQIKSIELTGGFLQGVKLEFGDGLNC
                     IIGGRGTGKTSVLEAIRYALDRMPDVHADKARYQAIEKLLDHNLGGGSVRVEIETADG
                     TTYTVTRGRGESPLVINAEGKPTEINIGKDIIFGVDLYSQNQIEDIANDVFFQLQLID
                     KFIHVEIDELEREIREATKLLEANATKILDARKAMGDLMEAIRELPDIAEKIRGFEKA
                     DQGGAGEVLRKEHEQRSLRSRELHLVSNVEALHATGTEALALVQRELRQKYEDAFDVD
                     LSASPNRDLIERIRDSTQTAIEAVERSLAAASKSLRAAQAELAPLKAQLTERHGGQDK
                     AYRELVDRHEQERVKGVERTRLEQRFADLKSKEKKLVEKKSALAALDREREQLRARLS
                     DLRDRRFQLRLGVAERLNQALAPMIRVRIEQFGNMDEYRELLNQSMKGSGLRYALIVD
                     RAVERIPPAELAAMIQREDRAGLERELDLDADRATRLIIQLKDKPELFAIETVELHDR
                     PILELRDGEDYKDSSTLSTGQKCTTILPILLVESERPLLIDQPEDNLDNAFVFETVVQ
                     SLAEIRDRRQLIFVTHNPNIPVLGDAQRVFVLRSSGRAASLASAGSVDEVAQDIITIL
                     EGGRAAFEARRKRYGRPVPVRET"
     gene            2956..3333
                     /locus_tag="VF516_27985"
     CDS             2956..3333
                     /locus_tag="VF516_27985"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8111615.1"
                     /translation="MKWPRELARKGDPDAQLRHRFGARVRALRDARGLTQEMIADRIE
                     VTPKYVSQLECGQRSPSWETLIAIAHQGFEIKLAALMFGIDEDIGVEVQYLSDLLAGR
                     PKQARRDLLRAVELMLRAGEGSR"
     gene            3360..>3639
                     /locus_tag="VF516_27990"
     CDS             3360..>3639
                     /locus_tag="VF516_27990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111616.1"
                     /translation="MLPASIPGILDVVLVRGARAPAGAALDLVIEVPHGATTTGDFTA
                     LAARLTSPLPDDLIAFFHVNTDAGAPELALATAHRLVADQPARSALVLR"
BASE COUNT          587 a         1219 c         1109 g          724 t
ORIGIN      
        1 gctcgcggta cgactgctcg ctgacctcca tcgcgatatc gtagatcagc gcgggcgtca
       61 gcgaccgcag gccgatctcg cgggtccgct tcgcgatgtg cttgaggatc ggatcctgta
      121 cgggcgcggt cagcgtccag agggtgtcgc gaccggtgcg ctcgcgaagc cggcgcacag
      181 cgcgtccggc agcctggctg acccagcggt tcacgtcacg cgcgagctca tcgaggacgg
      241 tctgaatgcg cgcttccggc cctcgaacct cggccagcgc caccgtcact gcctttcgaa
      301 cctcccattt cggatcatgc gcgagatcgc acaaggctgc aatcagcttt gtctcgtgat
      361 cctggctgag attgccactc gcgagtcgag cggtgaccgt ctgcaggcct gcaaccctcc
      421 gtgtccacga ggcatccctc agctcatcga agatctgctc gatgccggcg gcagaggggc
      481 gtccgctcat cacgtctccc tgaccggcac cggccgtccg taccgcttgc gccgtgcttc
      541 gaatgccgca cgaccgcctt cgaggatcgt gatgatgtcc tgggcaacct catcgacact
      601 tcccgcgctg gcaaggctgg cagcccggcc ggacgatcga agcacgaaca cgcgctgggc
      661 atcgccgagg accgggatat ttggattgtg cgtcacaaag atcaactgtc gccggtcgcg
      721 aatttctgca agactctgaa ccaccgtctc gaacacgaag gcattgtcga ggttgtcctc
      781 cggttgatcg atgagcagcg gtcgttcact ctcgaccagg aggatcggca ggatcgtcgt
      841 acacttctgg cccgtggaga gtgtactcga atccttgtaa tcctctccgt cgcgcagctc
      901 caggatgggg cgatcatgaa gctcgaccgt ctcgatggca aacagctcgg gcttgtcctt
      961 cagctggatg atcaatcgag ttgcccgatc cgcatcgaga tcgagctcgc gctccagacc
     1021 ggcgcggtct tctctctgaa tcatggctgc cagctcggca ggtgggatcc gctcgacggc
     1081 gcgatcgacg atcagcgcat agcgcaggcc ggagcccttc atcgactggt tgagcaattc
     1141 ccggtactcg tccatgtttc cgaactgttc gatcctcacg cggatcatcg gagccagcgc
     1201 ctggttgagc cgctcggcca ctccgagccg gagctgaaat ctgcgatcgc gaaggtctga
     1261 taaccgggcc cgaagctgtt cacgctccct gtcgagcgcc gcgagcgcag acttcttctc
     1321 gaccagtttc ttttcctttg acttcaggtc ggcgaaacgt tgctcgagcc gcgtccgctc
     1381 cacccccttg acgcgctcct gctcatggcg atcgacgagc tcgcgatatg ccttgtcctg
     1441 gcccccgtgg cgctccgtca gctgggcctt caacggcgcg agctcggcct gggcagcgcg
     1501 aagactcttg ctggcggctg ccaggctgcg ctcgacggct tcgatcgccg tctgcgtgct
     1561 gtcgcgtatt ctctcgatga gatcgcgatt cggcgacgca gacaggtcca cgtcgaacgc
     1621 gtcctcgtat ttctgtctga gctcgcgctg aaccagggcc agtgcttccg tgcccgtcgc
     1681 atggagtgcc tcgacgttgc tcaccaggtg cagctctcgg ctcctcaggc ttcgttgttc
     1741 gtgctccttc cgcaacacct cgccggcacc gccctggtcg gctttctcga agccgcggat
     1801 cttttcggca atgtcaggaa gctcccggat cgcctccatc agatctccca tcgccttccg
     1861 tgcgtcgagg atcttggtcg cattggcctc gagtagcttt gtcgcctcgc ggatctctcg
     1921 ctcgagctca tcgatctcga cgtgaatgaa cttgtcgatg agctgaagct gaaagaacac
     1981 gtcgttggcg atatcctcga tctggttctg actgtagaga tcgacgccaa agatgatatc
     2041 cttgccgatg ttgatctcgg tgggtttccc ctccgcgttg atgacgagcg gagactcccc
     2101 gcgccctctg gtcaccgtgt aggtcgttcc gtcggcggtc tcgatctcca cgcgaacact
     2161 cccgccgccc aggttgtgat cgagcagctt ctcgatcgcc tggtaccttg ccttgtcggc
     2221 atggacgtcc ggcattcgat cgagagcgta ccggatggcc tcgagcacgc ttgtcttgcc
     2281 ggtgccgcgc ccaccgatga tgcagttcag gccatcgcca aactccagct tcacgccctg
     2341 caagaaacct ccggtcaact cgatggactt gatctggtga tgcgcggttg cagctggccc
     2401 ggatgaaccg gcgttctcgt tgcgagactt catcgtgcat gtccctggac gacctggcgg
     2461 gtctgcacgg ctaccgtacg tcagaagcgc tcgcccgcga ggcggtcgga ctcctcggtg
     2521 tcacgttttt cgacggacgt gtcacatctt tcctgaaaat cgcaagaatg acaagtagat
     2581 agagagcttg cctgtcacgt gtttccaggg cccgtgggta tcttcctcgc cggtcggctc
     2641 caagcttctg tccatccagc caaccaacag caggaagaaa cagtggcacc tgcaaccgag
     2701 agcgccaatg agacagcgct caaccggcca gtgcactccc ttgcaacgct ctccgtcgca
     2761 gaggatccgc ccgacgggcc actgggacct ccgagggaac atgctcgatc tcgcgcccat
     2821 ctgtccttgc tctcgcagct accctccttc cgggaggtcc gagctcagcg cagtgtcccg
     2881 gcgccctccg tggaacgcct gtgcgctgga tccgagcggg gcgaatggca ccacgaacaa
     2941 ctcgcgggtc acgtcatgaa gtggccccgc gagctcgcgc ggaagggtga tcctgatgct
     3001 cagctccggc atcgcttcgg tgcgcgggtc cgagcgcttc gcgatgctcg cgggctcacg
     3061 caggagatga tcgcagaccg catcgaggtc acgccgaaat acgtgagcca gctcgagtgc
     3121 ggccagcggt cgccgagctg ggagaccttg atcgcgatcg cgcaccaggg cttcgagatc
     3181 aagctcgccg cgctgatgtt cgggatcgac gaggacatcg gggtcgaggt gcagtacctg
     3241 agtgacctgc tcgccggccg gcccaagcaa gctcgccgcg acctgctgcg cgccgtggag
     3301 ctgatgctgc gcgcggggga aggatcgagg tagcggcgtg cggtaggctg gcggcgccca
     3361 tgctgcccgc ctcgattccc gggatcctcg atgtcgtgct ggtccgcggt gcgcgagcgc
     3421 ccgccggcgc ggcgctcgat ctggtgatcg aggtcccgca cggcgcgacc accaccggcg
     3481 acttcacggc gctggcggcc cggctgacca gcccgttgcc cgacgacctg atcgcgttct
     3541 tccacgtcaa caccgacgcc ggcgcgcccg agctcgcgct ggcgaccgcg caccggctgg
     3601 tcgccgatca gcccgcccgg tcggccctcg tcctgcgct
//
LOCUS       DASVOR010001481         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_219781,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001481 DASVOR010000000
VERSION     DASVOR010001481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219781"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF516_27995"
     CDS             <1..447
                     /locus_tag="VF516_27995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111617.1"
                     /translation="SRAAPGDAQAEDTQADRPPSGDCELVLGLELGGLVHRYQATVRW
                     GARAWFRDEPYAIELRGGHLPQAAPTARHHPQLWGLLRDRARKALSSALAVATLVHYG
                     VTSVTDAMVADLIARGQDAALHALLADGARDVPREAWERFVRAQTS"
     gene            957..1739
                     /locus_tag="VF516_28000"
     CDS             957..1739
                     /locus_tag="VF516_28000"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8111618.1"
                     /translation="MVRDEEQEDPPTTNRPAPSPPPPLATAKRPEIDAPAARLVDMSK
                     QRHIAVPCDQFTIGRSSNSNLVLKDICVSRQQAVITCKDGQFFISSVGKAPVLVNEDP
                     GGVFEQKYLALQNEPVSATNLPLHMSNVKQELLNTGPSAEETAKRVQSLAEQAIRSKQ
                     LAETRARKENKNNTNSTNFARQFFMHKHKLNDSEKKKVSELSKEMFARLQNLCEQGAN
                     ARVVLDKESYKEPKYGNALEILASRGNNPLTQSAYRRLTEQE"
BASE COUNT          389 a          792 c          746 g          314 t
ORIGIN      
        1 tcccgcgccg cgcccggcga cgcgcaggcc gaggacaccc aggccgaccg cccgccgtcc
       61 ggcgactgcg agctggtgct cggcctcgag ctcggcggcc tggttcaccg ctaccaggcc
      121 acggtgcggt ggggcgcgcg cgcctggttc cgcgacgagc catacgccat cgagctccgc
      181 ggaggccacc tcccccaggc ggcgccgacc gcgcgccacc acccccagct gtggggcctg
      241 ctgcgcgatc gcgcgcgaaa ggcgctgtcc tccgcgctgg cggtcgccac cctggtccac
      301 tacggcgtca cgtcggtgac cgacgcgatg gtggcggacc tgatcgcgcg cggccaggac
      361 gccgcgctgc acgcgctgct cgccgacggc gcccgcgatg tcccgcgcga ggcctgggag
      421 cgcttcgtcc gtgcgcagac gtcatagccg gttgccggag agagccctcg atgctgatca
      481 tgacgagagc gagacagccc ctggtgcagc aaggctacgt tcgcgccgag cgtcatccag
      541 cgtgggcgat gacggtccat cccggcgctg tcgcgcgacc cgccacccaa aacctgccgc
      601 tccatggggc cgggcatcgg gtgctgtcca tcgcgaggtg gcgacgatga cgaacggcct
      661 ggtgctgcag gggtacttcc gtctcgagcg agttcccgca ccggcgctga gtacgccgct
      721 cgcccggccc gatctgcgtt ccgtcccgac gcgcacgcac gcagcgcagc ccagactcgg
      781 cggcgagctc ggcgccgcct ggcccggggc gcccaggccc gatctgctcc cgggcgtggg
      841 cctctcgcgc gtgggcgccg tgcagcgtat cgcccgggct cgacccggtg acgcggtcgc
      901 aacgacgccg atcagcgcgg cacaggttca ggcgattggc ccgggagcac ccgatcgtgg
      961 tgcgcgacga agaacaagaa gatcccccga cgacgaatcg ccccgctccg tcgccaccgc
     1021 cgccactcgc gacggcgaag cggcccgaga tcgatgcgcc tgcggccagg ctcgtcgaca
     1081 tgtccaaaca gcggcacata gcggtgccat gcgaccagtt cacgattggc cgtagctcaa
     1141 actccaatct tgtgctcaag gacatatgcg tctcgcgcca gcaggcagtc atcacgtgca
     1201 aggacggcca gttcttcatt tccagcgtcg ggaaagctcc ggtgctggtg aatgaagatc
     1261 ccgggggcgt gttcgagcag aagtatctgg cgcttcaaaa cgagccggtg agcgccacga
     1321 acctgccgct gcacatgagc aacgtgaagc aagagctgct gaatacgggt ccgagcgcgg
     1381 aggagacggc taagcgcgtg cagtccctcg cggagcaagc catccgaagc aagcagctcg
     1441 ccgagacacg cgcgcgcaag gaaaacaaga acaataccaa ctcaacgaac ttcgctcgcc
     1501 agttcttcat gcacaagcac aaactcaatg acagtgagaa gaaaaaggta tccgagctaa
     1561 gcaaagagat gttcgctcga ttgcaaaatc tctgtgagca gggcgcgaac gcacgcgtgg
     1621 tcctggacaa ggaatcttat aaagagccca agtacgggaa cgcgctcgag attctggcgt
     1681 cacgcggcaa taacccgctc acgcagagcg cgtaccggcg gctcaccgag caggagtgag
     1741 cctggcaggc aacggcccgc ttgcatcgcc gaagtagacc caggccagct ccgtgaggat
     1801 cacgatctga gtctgccacg cgccttgccg ggcgcttgcc gtggcgccga tgtggccggt
     1861 ccgccggggt cgttcggtcc gaccccagac ctggctccgc gcagcgcttc gccgagcggt
     1921 ggcgctgccg gacgggtggc gtgagcgctg acccgagtgg ccacggtaga cggcgtttgg
     1981 ctcctgggtt acgctcgaac agaccatcgc cctggacgca ggatgctcgc tccaccagat
     2041 gcacctcatg acgccgcgcg ggccgttcag acggtgccga gccgcctggg ccatgcctgg
     2101 ctcgccatca tgcgccggga cctcttcgcg ggagtcctgc catgagcgcc acggcgagcc
     2161 gcgcggtcat catccaggcc agctttcccg tcgcggcgcc cgcgaccggc gcgaccgccg
     2221 cgccgggcac tgccggaccc g
//
LOCUS       DASVOR010001482         1622 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_453279,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001482 DASVOR010000000
VERSION     DASVOR010001482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453279"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VF516_28005"
     CDS             <1..864
                     /locus_tag="VF516_28005"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8111619.1"
                     /translation="WGVRGSIPAPGPDTIRYGGNTACVSVHARSGGLVILDMGTGLMH
                     LGNALMAGPFGDGRGAATILLSHTHWDHIQGLGFFSPVFVAGNAFTVYGPGGSSGVLE
                     DILEGQMDPNFSPLQTLKNFSARFDVHAIELGAPLSAGGLTITAREHPHGSTTALAFR
                     ITEPAAPATGAPERTFVYASDVGQPTAAGPDPDAIAFYRGADVLLHDATYRPADQATR
                     RNRGFSSYEDAADAAVASGARRLVTFHSDQDYTDDDVDDLVASCRRALDARGGAAIDL
                     VPAREGDELEI"
     gene            complement(892..>1622)
                     /locus_tag="VF516_28010"
     CDS             complement(892..>1622)
                     /locus_tag="VF516_28010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111620.1"
                     /translation="SSGVGGRDWYLHATGGCKDTSDVVGLRRCTRFAAWAKNLEYPQL
                     ILEGGALVRRFPSLLGSQIGSVTHGVESFSYRMIQPASVHRFDTALLASVRVSIGLSR
                     AVYGGLEVDLGGITRPGPAAEMASTGVFGSPDVQPGHGLLVDSLGVVGVRAATGFGAL
                     GVELAGGLRAVSYSFHSSYHDCQQSTSVNALAAVGEARARGELWLGPWLTAGVTLGTS
                     VLERNAWMGGVYLGVHSRAFGGNR"
BASE COUNT          214 a          650 c          554 g          204 t
ORIGIN      
        1 tggggcgtcc gcggctcgat cccggcgccc ggccccgaca cgatccgcta cggcggcaac
       61 accgcgtgcg tctcggtcca cgcccgctcc ggcggcctgg tcatcctcga catgggcacc
      121 ggcctcatgc acctgggcaa cgcgctgatg gccggcccgt tcggcgacgg ccgcggcgcg
      181 gcgacgatcc tcctgtccca cacccactgg gatcacatcc agggcctggg cttcttctcc
      241 cccgtgttcg tcgccggcaa cgcgttcacc gtgtacggcc cgggcggctc gtccggcgtc
      301 ctcgaggaca tcctcgaggg ccagatggat cccaacttct cgccgctcca gaccctcaag
      361 aacttctcgg cccggttcga cgtccacgcg atcgagctcg gcgcgccgct gtccgccggg
      421 ggcctcacca tcaccgcccg cgagcacccg cacggctcga ccaccgcgct cgcgttccgg
      481 atcaccgagc cggcagcccc cgccaccggc gcgcccgagc gcaccttcgt ctacgcctcc
      541 gacgtcggcc agcccaccgc cgccggcccc gaccccgacg ccatcgcgtt ctaccgcggc
      601 gccgacgtgc tgctccacga cgcgacctac cggcccgccg atcaggcgac ccgccgcaac
      661 cgcggcttct cgtcctacga ggacgccgcg gacgccgccg tcgcctccgg cgcccgccgc
      721 ctggtcacct tccactccga ccaggactac accgacgacg acgtcgacga tctcgtcgcc
      781 agctgccgcc gcgccctcga cgcccgcggc ggcgccgcga tcgacctcgt ccccgcgcgc
      841 gagggcgacg agctggagat ctgatccgcc ggggcgggcg ccggaccgtg atcagcggtt
      901 gccgccgaac gcgcggctgt ggacgccgag gtagacgccg cccatccacg cgttgcgctc
      961 gagcacgctg gtgccgagcg tgacgccggc ggtgagccac gggccgagcc acagctcgcc
     1021 gcgcgcgcgg gcctcgccga cggcggcgag cgcgttgacg ctggtcgact gctggcagtc
     1081 gtgatagctg ctgtggaagc tgtaggacac cgcgcgcagg ccgccggcga gctcgacgcc
     1141 gagcgcgccg aacccggtcg cggcgcggac gccgacgacg ccgagcgagt cgacgagcag
     1201 gccgtggccc ggctggacat cggggctgcc gaacacgccg gtcgacgcca tctcggccgc
     1261 cggtcccggc cgggtgatgc cgccgagatc gacctcgagg ccgccgtaca ccgcgcgcga
     1321 caggccgatg ctgacgcgca cgctggccag cagcgcggtg tcgaaccggt ggacgctggc
     1381 gggctggatc atgcggtagc tgaaggattc gacgccgtgg gtgacgctgc cgatctggct
     1441 cccgagcagg ctcgggaacc gccggaccag cgcgccgccc tcgaggatga gctgcgggta
     1501 ctcgaggttc ttggcccacg cggcgaaccg cgtgcagcgg cggagcccga cgacgtcgct
     1561 ggtgtccttg cagccgccgg tcgcgtgcag gtaccagtcg cggccaccga cgccgctcga
     1621 gc
//
LOCUS       DASVOR010001483         3420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_394378,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001483 DASVOR010000000
VERSION     DASVOR010001483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394378"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VF516_28015"
     CDS             complement(<1..1124)
                     /locus_tag="VF516_28015"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX8111621.1"
                     /translation="MSDGTVDAFTSEQFDLIKHFGPLPDLPPDTTNRYADNPDAAVFG
                     QQLFFEKSYAGPLTIADPVLGSVGQPGKVACASCHDVTHYWTDTRSKPNNMSLGAKWT
                     TRNAPTLVNVAYYQWNSWGGKEDSLWYQGANGCESAVNFAGNRLQYAHMIYVKYRAQY
                     NALFQPSLDSALDPADTSGRFPPNGAPGTAGSALDTAWRGMTAGDQDIVNTIMANVGK
                     SIEAYERKLISRNAPIDKYIAGDYTALTPAAKRGLALFIGKAACVDCHSGPTLSDNSF
                     HNTGVPQAGINTPVTDNGRFDEIPKTLSNNFNTWSKYSDDQDLGRAKMATLAQTPDLK
                     GAFRTGMLRQIEMTGPYMHTGSLETLEDVVRFYNWGGGDA"
     gene            complement(1201..2163)
                     /locus_tag="VF516_28020"
     CDS             complement(1201..2163)
                     /locus_tag="VF516_28020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111622.1"
                     /translation="MTALHRLAYTTVALCACGGARPAAAPGDTAPGGPPITTLATAQG
                     YPINIRGDAGGLFWTNHLGGQIVALAAGGAPKVLADHQDYPLGLAIDQTDVYWTTLNG
                     GTVVKVARAGGQPVTIASGQFGPSGIAIDDTNVYWTNTYGGTIVTVSKAGGKPTVLAS
                     KQATPAEVAVAAGVVYWTNRGSAPDYADGSVMRLPKGGAPAAVATALADPQGIAVDGT
                     AIYWLNHGSPPQFHGAVMTAPLDGGAPTVLVPDQQGAQELAIDDTHLYWSNEVEGSVN
                     RVAKRGGAPTTLARNQQRPYGVCVDTTYVYWLAYRDGSAKRMAK"
     gene            complement(2173..2652)
                     /locus_tag="VF516_28025"
     CDS             complement(2173..2652)
                     /locus_tag="VF516_28025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111623.1"
                     /translation="MLGQISLVALVGLSACSSPAAGPRLGRDPSKTTELGLFMKTRVN
                     PSFSKISFLLFHDEDSDTEVDPTALPASANELASAAARLSTWTNLPGESEQSKQVFDE
                     YAESLKTDAVKLVDSLRGKQLDNARKIFESLHRKCDACHHFFRYDQSTVLDTRTPRP"
     gene            2848..>3420
                     /locus_tag="VF516_28030"
     CDS             2848..>3420
                     /locus_tag="VF516_28030"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111624.1"
                     /translation="MPIAGLDAACRDGVASVSTGWSHSCALCKSGEVACWGTNFLGNL
                     GVPLPTERPTPAIIAGLKDVKEVAAGSYHTCARLASGRATCWGANNDGQLGDDTEATR
                     LRPVMVSDLKDAQQIDVGLGPGHSCAVVKSGKVRCWGVNFNSQLGDGTMTNHASPVDV
                     DIIDDATQVSTGAYHTCALRKSGKVACWGGK"
BASE COUNT          489 a         1107 c         1225 g          599 t
ORIGIN      
        1 ttggcatcgc caccgcccca gttgtagaac cgcacgacgt cctccagggt ctccaggctg
       61 ccggtgtgca tgtacggtcc ggtcatctcg atctggcgca gcatgccggt gcggaacgcg
      121 cccttcagat cgggggtctg cgcgagggtc gccatcttgg ccctgccgag gtcctgatcg
      181 tcgctgtact tgctccaggt gttgaagttg ttgctcagcg tcttggggat ctcgtcgaac
      241 cggccgttgt cggtgaccgg cgtgttgatg ccggcctggg ggacgccggt gttgtggaaa
      301 ctgttatccg acagcgtggg gccggagtgg cagtcgacgc acgccgcctt gccgatgaac
      361 agcgcgaggc cgcgcttggc ggcgggcgtc agcgccgtgt agtcgcccgc gatgtacttg
      421 tcgatcggcg cgttgcggct gatcagcttg cgctcgtagg cctcgatcga cttgccgacg
      481 ttggccatga tcgtgttgac gatgtcttga tcgcccgcgg tcattccccg ccacgccgtg
      541 tcgagagcgg acccggcggt cccgggcgcg ccgttcggcg ggaatcgccc gctcgtgtcg
      601 gcgggatcga gcgcggaatc gagcgacggc tggaacagcg cgttgtactg ggcgcggtac
      661 ttcacgtaga tcatgtgggc gtactgcagg cggttcccgg cgaagttgac cgcgctctcg
      721 cagccgttgg cgccctggta ccacagggag tcctccttgc caccccagct gttccactgg
      781 tagtacgcga cgttgaccag ggtcggcgcg ttgcgcgtcg tccacttcgc gccgagcgac
      841 atgttgttgg gtttcgaccg ggtgtcggtc cagtagtggg tgacgtcgtg gcagctcgcg
      901 cacgcgacct tgccgggctg gccgaccgag ccgagcacgg gatcggcgat cgtgagcggg
      961 cccgcgtagc tcttctcgaa gaacagctgc tggccgaaca ccgcggcgtc cgggttgtcg
     1021 gcgtaccggt tggtcgtgtc gggcggcaga tcgggtagcg gtccgaaatg cttgatgagg
     1081 tcgaactgct ccgacgtgaa cgcatcgacg gttccgtcgc tcatgcaccc ggtgagcgcc
     1141 agggtcgccg caacaacaga gcacaggtta gagcgcatag gtcaccatga gtaagaggtg
     1201 ttattttgcc atccgcttgg cagaaccatc gcggtaagcg agccagtaga catacgtggt
     1261 gtcgacgcac acaccatagg gacgttgttg attgcgcgcc agcgtggtcg gtgcgccgcc
     1321 gcgcttggcg acgcggttga cgctgccctc gacctcgttg gaccagtaca ggtgggtgtc
     1381 gtcgatcgcg agctcctggg cgccctgctg gtcggggacc aggacggtcg gcgcgccgcc
     1441 gtcgagcggc gcggtcatga cggcgccgtg gaactgcggc gggctgccgt ggttgagcca
     1501 gtagatcgcg gtgccatcga ccgcgatgcc ctggggatcg gcgagcgcgg tggcgaccgc
     1561 ggccggcgcg ccgcccttgg ggaggcgcat gaccgacccg tcggcgtagt ccggagccga
     1621 gccgcggttg gtccagtaca cgacgcccgc ggcgaccgcg acctcggcgg gcgtggcctg
     1681 cttggacgcg agcacggtcg gcttgccgcc ggccttgctc acggtcacga tcgtgccgcc
     1741 gtacgtgttg gtccagtaca cgttggtgtc gtcgatcgcg atcccgctcg gcccgaactg
     1801 gcccgacgcg atcgtgaccg gctggccgcc ggcccgggcg accttgacca ccgtcccgcc
     1861 gttcagcgtg gtccagtaca catcggtttg atcgatcgcg aggccgagcg ggtagtcctg
     1921 gtgatcggcg aggaccttcg gcgcgccgcc cgccgccagc gcgacgatct gaccgccgag
     1981 gtggttggtc cagaacaggc cgccggcgtc gccgcggatg ttgatggggt agccctgcgc
     2041 ggtggccagc gtggtgatcg gcggtccgcc cggtgcggtg tcccccggag cggcggccgg
     2101 cctggcgccg ccgcacgcgc acagcgcgac ggtcgtgtac gccaggcggt ggagcgcggt
     2161 cattgcatca cctcagggcc tcggggtccg cgtgtccagc accgtcgact ggtcgtagcg
     2221 gaagaagtgg tgacaggcgt cgcacttgcg gtgcagcgac tcgaagatct tgcgcgcgtt
     2281 gtcgagctgc ttgccgcgca gcgagtcgac cagcttgacc gcgtcggtct tcagcgactc
     2341 ggcgtactcg tcgaacacct gcttgctctg ctccgattca cccggcaggt tggtccaggt
     2401 gctgagccgc gcggccgcgc tggcgagctc gttcgcgctc gccggcagcg ccgtgggatc
     2461 gacctccgtg tcgctgtcct cgtcgtggaa cagcaggaac gagatcttgc tgaacgacgg
     2521 gttcacgcgg gtcttcatga acagaccgag ctcggtggtc ttggagggat cgcggccgag
     2581 ccgcggcccc gcggcggggc tggagcacgc cgacaggccg accagcgcga cgagcgatat
     2641 ctgcccgagc accgattcgc gtcgtctttt tggaacttgg gtattcaagg gatgtgcact
     2701 tcgcgccatg actgatatca tcgtgcctcg atgctgccat tcaaacactt gttgatcgcg
     2761 ccgaccgcag tgatcgcgat tggctgtgga gcaagctctg caccccgctc gtccaaccct
     2821 gctgcggcgc agcggatctc gccgcccgtg ccgatcgccg gcctcgacgc cgcatgtcgc
     2881 gacggcgtcg ccagcgtctc gaccgggtgg tcgcacagct gcgcgctgtg caagagcggc
     2941 gaggtcgcgt gctggggaac caacttcctg ggcaacctgg gcgtgccgct gcccaccgag
     3001 cgaccgaccc ccgcgatcat cgcggggctc aaggacgtca aggaggtcgc ggcgggtagc
     3061 tatcacacct gcgcgcggct cgcgtcgggg cgcgcgacct gctggggcgc gaacaacgac
     3121 ggccagctcg gcgacgacac cgaggcgacc cggctgcgcc cggtgatggt gagcgatctc
     3181 aaggacgcac agcagatcga cgtcgggctc ggccccggcc acagctgcgc ggtggtcaag
     3241 agcggcaagg tccgctgctg gggcgtcaac ttcaactcgc agctcggcga cgggacgatg
     3301 accaaccacg cctcgccggt cgacgtcgac atcatcgacg acgcgaccca ggtctcgacc
     3361 ggcgcgtatc acacctgcgc gctgcgcaag tcgggcaagg tcgcgtgctg gggcggcaag
//
LOCUS       DASVOR010001484         3212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_541198,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001484 DASVOR010000000
VERSION     DASVOR010001484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541198"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="VF516_28035"
     CDS             complement(<1..356)
                     /locus_tag="VF516_28035"
                     /inference="COORDINATES: protein motif:HMM:NF020076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX8111625.1"
                     /translation="MKVAIRPWPCVAVVCLLSGAAHATRPRPDPADSVREGRLVTQRG
                     GARIDVPLQHTDVKITVDGFLAEATVTQRFKNPYPSKIEAVYLFPLPTGAAVSALQIT
                     SGDRMIEGTIQERRQAT"
     gene            521..1204
                     /locus_tag="VF516_28040"
     CDS             521..1204
                     /locus_tag="VF516_28040"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8111626.1"
                     /translation="MATTEHRVATHLGLAASDYDAQIRRYTPRYDEMIDTVVAIIGAL
                     DAPYIIDLGAGTGALGGAILDRVPRARVRFLDIDPAMLEVAGARVAAHGARAELCRGS
                     FDDALPACDAVVASLALHHVPERDRKRALYARVHEALRPGGVLLVADITVHETGIAHD
                     HVYRDWIASMASHGIAEPEARALLAQWAGEDRYYPLAAELAMLGAAGFAQPECFWKHG
                     PSTVFGGFR"
     gene            1419..2957
                     /locus_tag="VF516_28045"
     CDS             1419..2957
                     /locus_tag="VF516_28045"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8111627.1"
                     /translation="MRGVAVCCGFNGYDVRPLDRAVNDAAILYAKLTESGAFNTAPDL
                     IGKGEMFTQRTSANAILAAFTRAALSPAELVWFSFSGHAIVSDDGELRLLLPGWRREA
                     SAEHQRAYSIGASEIENVLRSRLADKKFVVLLDACYSGAFGAAAVSRDIDPGLPAIEE
                     RLAGAGAVVISSCARDQLAYDCHPTVNRPNGAFTAAVIEVLDEHVRAGAPLSVLQLFQ
                     EAKDKLRNGQLPTLYVNGLTDDFPILERVRPEAPGDGIVPLSAEVPAALTHELHTFLH
                     SVVQIRRRRRVGLVRATRQLERLAAEFYRYGDDAFVVPGHDANVAEAFDNARTCIVGC
                     TTPAYVDEWRRGGAAVLATNRDLVARHGGRVVRFFFVPDDFESRVPGVLAVVRDHLEA
                     GVLAVVVNVDSFGPAVLQEVFKEPRPSDLSSLECAFVDGRIFLRTHFVANGDPRIELD
                     QRPARCRHEYKTQLRPFLVAGGALFGASLRDGGGEGVELFPLQLGDVDELREQLDADL
                     GVAA"
     gene            complement(2984..>3212)
                     /locus_tag="VF516_28050"
     CDS             complement(2984..>3212)
                     /locus_tag="VF516_28050"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8111628.1"
                     /translation="LQALAPAGARQAVHAHVVRGDDTATTIAQCAARFGVDVICIASH
                     GRSGISRALMGSVADSLLRATRLPVLVLRPA"
BASE COUNT          435 a         1107 c         1179 g          491 t
ORIGIN      
        1 gtcgcctgcc ggcgctcctg gatcgtcccc tcgatcatcc ggtcgccgct ggtgatctgc
       61 agcgcgctga ccgcggcgcc ggtcggcagc gggaacaggt acaccgcctc gatcttcgac
      121 ggatacgggt tcttgaaccg ctgggtcacc gtggcctcgg cgaggaagcc gtcgacggtg
      181 atcttcacgt cggtgtgctg gagcggcacg tcgatccggg cgccgccgcg ctgggtgacg
      241 agccggccct cgcggaccga gtcggccgga tcgggccggg gccgcgtcgc gtgtgcagcg
      301 ccggacagca ggcagacaac ggccacgcag ggccacggcc ggatggcgac cttcatgggg
      361 ctccctttct gcgtccgccg gcaaaggcgg caacagtcac cggctcggac accgcgctcg
      421 ccgatcggtt tccgcgaatc tcgcgccccg cgcaaaagtg ccggccggca cgggcgccat
      481 ctgtaccggt cgcgcggatc ggtccgggct acggtggtgc atggcgacca ccgagcaccg
      541 tgtcgcgacc cacctggggc tcgcggccag cgactacgac gcgcagatcc ggcgctacac
      601 gccgcgctac gacgagatga tcgacaccgt ggtcgcgatc atcggcgcac tcgatgcgcc
      661 gtatatcatc gacctcggcg cgggcaccgg cgcgctcggc ggggcgatcc tcgatcgcgt
      721 gccgcgggcg cgggtccggt tcctcgacat cgaccccgcg atgctggagg tcgccggcgc
      781 gcgggtcgcg gcgcacggcg cgcgggccga gctctgccgg ggctcgttcg acgacgcgct
      841 gccggcgtgc gacgcggtgg tcgcctcgct cgcgctgcac cacgtccccg agcgcgatcg
      901 caagcgcgcg ctgtacgcgc gggtccacga ggcgctgcgg cccggcggcg tgctgctggt
      961 cgccgacatc acggtgcacg agaccggcat cgcccacgac cacgtgtacc gcgactggat
     1021 cgcgagcatg gcaagccatg gcattgccga gcccgaggcc cgggcgctgc tcgcacagtg
     1081 ggccggcgag gatcgctact atccgctcgc cgccgagctg gcgatgctcg gcgccgccgg
     1141 cttcgcccag cccgaatgtt tctggaagca tggcccatcg acggtgttcg gcggcttccg
     1201 ctgatcgacg cgtcttacct cagatccaga gtaggtcttg cagcggattc gtcgcttcgg
     1261 cgagctgctg tctgcagcat tgcagtgaaa ctggatatca gcgtgtcggc cgcagatccg
     1321 acagtgatca gcggcgcggt gtggatgagc ggatggtgag atcatcgccg cgcctgtcgc
     1381 gggacctcac gatgaaggga ttggggggag gtttgaccat gcgtggtgtc gcagtgtgtt
     1441 gcggcttcaa tggctatgac gtcaggccgc tcgatcgcgc ggtgaacgat gcggcgatcc
     1501 tgtatgcgaa gctcaccgag agcggggcgt tcaacacggc gcccgatctg atcggtaagg
     1561 gcgagatgtt cacgcagcgc acgtcggcga acgcgatcct cgcggcgttc acgcgcgccg
     1621 cgctgagccc ggccgagctg gtgtggttct cgttctccgg tcacgcgatc gtctccgacg
     1681 acggcgagct gcgcttgctg ctgcccggct ggcggcgcga ggcgagcgcg gagcaccagc
     1741 gcgcgtactc gatcggagcc agcgagatcg agaacgtgct gcggtcgcgc ctcgcggaca
     1801 agaagttcgt ggtcctgctc gacgcctgct acagcggggc gttcggcgcc gccgcggtca
     1861 gccgggacat cgatccaggg ctccccgcga tcgaggagcg gctcgccggg gccggcgcgg
     1921 tcgtgatctc gtcgtgcgcc cgcgatcagc tggcctacga ttgccatccc accgtgaacc
     1981 ggccgaacgg cgcgttcacc gcggcggtga tcgaggtgct cgacgagcac gtgcgggcag
     2041 gagccccgct cagcgtcctg cagctgttcc aggaggccaa ggacaagctg cgcaatggcc
     2101 agctgcccac gctgtacgtg aacgggctca ccgacgactt tccgatcctc gagcgcgtcc
     2161 ggcccgaagc gccgggcgac gggatcgttc ctctgtcggc cgaggtcccg gccgcgctca
     2221 cccacgagct gcacacgttc ctccacagcg tggtgcagat ccgccgccgg cgtcgggtcg
     2281 ggctggtccg cgcgacccgc cagctcgagc ggctcgcggc cgagttttat cgctacggcg
     2341 atgatgcgtt cgtggtgccc ggccacgacg ccaacgtcgc cgaggcgttc gacaacgcgc
     2401 gcacgtgcat cgtcgggtgc accacgccgg cgtacgtcga cgagtggcgg cgcggcggcg
     2461 cggcggtcct ggcgacgaac cgggacctcg tggccaggca cggcggccgc gtggtccggt
     2521 tcttcttcgt gcccgacgac ttcgagagcc gcgttccggg cgtgctcgcg gtcgtgcgcg
     2581 atcacctgga ggccggggtc ctcgcggtgg tcgtcaacgt cgactcgttc ggcccggccg
     2641 tgctgcaaga ggtgttcaag gaacccaggc cgagcgatct cagctcgctg gagtgcgcgt
     2701 tcgtcgatgg caggatcttc ctcaggaccc acttcgtggc caatggtgat ccgcggatcg
     2761 agctcgacca gcggccggcg cggtgccggc acgagtacaa gacccagctg cggccgttcc
     2821 tcgtcgcggg tggcgcgctg ttcggcgcca gcctgcgcga cggcggcggc gagggcgtcg
     2881 agctgttccc gctgcagctc ggcgacgttg acgagctccg cgagcagctc gacgccgacc
     2941 tcggcgtggc ggcgtgattc gccggccgcc ccgcgtcgcc tcgtcaggcc gggcgcagca
     3001 cgagcaccgg caggcgggtg gcgcgcagca ggctgtcggc gaccgagccc atcagcgcgc
     3061 ggctgatccc ggagcggccg tgcgaggcga tgcagatcac gtcgacgccg aaccgcgcgg
     3121 cgcactgggc gatcgtcgtg gcggtgtcgt cgccgcgcac gacgtgggcg tggaccgcct
     3181 ggcgggcgcc ggccggggcg agcgcctgga gc
//
LOCUS       DASVOR010001485         2884 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_131839,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001485 DASVOR010000000
VERSION     DASVOR010001485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131839"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VF516_28055"
     CDS             <1..238
                     /locus_tag="VF516_28055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111629.1"
                     /translation="VHDDGEMWSASLFQIRTALGAARADQLILQAHFLLSTRANFSDG
                     SNALVTAAINLGFTAAEVTNLRTILQSRGFTVTA"
     gene            290..862
                     /locus_tag="VF516_28060"
     CDS             290..862
                     /locus_tag="VF516_28060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111630.1"
                     /translation="MKHGPIVLAAIGAAGASAGTARAECPTTPDDAVCRPWSAMLLPT
                     GFAAVYAPHGLDATFYGGGLEAVLLAWNDNSDAYGPSQGRLRIDVAYLTSSNAMTGGM
                     AMYRGGAQLAFERNASRSYGIPYFAADLGGLWSDATGRRWYVDGGVGLYLVHRRNVIV
                     DLEVTGVLPFRDPGTLGGVRSRLGLSFALW"
     gene            939..1355
                     /locus_tag="VF516_28065"
     CDS             939..1355
                     /locus_tag="VF516_28065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111631.1"
                     /translation="MNCHPSGDAPLQYDDSRLHGMAISRRSEANGVACATCHRDRNDG
                     RPGQPPGAPSWHLPPADTPMIFQGRTPHQLCLQLLDPRQTRGRDVAALIEHVAHDELV
                     GWGWAPGAGRTPVPVPRADVVAAMQAWADAGTPCPE"
     gene            complement(1366..1947)
                     /locus_tag="VF516_28070"
     CDS             complement(1366..1947)
                     /locus_tag="VF516_28070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111632.1"
                     /translation="MAFRLLAVAMLVGACGTVTNSPADAAVGDGGRPDGAAGAVTWRN
                     QPGDVPLVMFGGTAADGAMYCKYTITLRQLDVQLAILPSGQVTSGHVQDLNVEAVVPS
                     SPPTTPFVCTATNPPPIPANIAMYQLATATATTGGMTLTFQDGASNMPQATLVIVLTK
                     VNSLYSAKLTFHRNDGLDPVFEWMVTTTVPLAP"
     gene            complement(2070..>2884)
                     /locus_tag="VF516_28075"
     CDS             complement(2070..>2884)
                     /locus_tag="VF516_28075"
                     /inference="COORDINATES: protein motif:HMM:NF040596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MXAN_2562 family outer membrane beta-barrel
                     protein"
                     /protein_id="HEX8111633.1"
                     /translation="AATGAIFVLGMGRAHAGNGGYQPYWEDTDPTRQAEQAQVPPGDP
                     SLVEWHAGLRIGPYTPDIDNQLDMHPGPFQQMFGTTQHMMTMLDVDRFLWTGFGQVGV
                     GVSVGYWQKTARALGAQPDGTPTSMRDASAHNAMRLVPLALTATYRFTVLDDNYGIPV
                     VPYARAGLGYYVWWISINDHFARVCSDGGLEPNCSQNKALGASLGLQGAIGLAIRAER
                     IDASAAMSMQQSGIQHAGIYAELSLAKMDGFGSDQKLSVGDRTWFAGVDFEF"
BASE COUNT          413 a         1033 c         1009 g          429 t
ORIGIN      
        1 ggtccacgac gacggcgaga tgtggtcggc ctcgctgttc cagatccgga cggcgttggg
       61 cgccgcccgg gccgaccagc tgatcctcca ggcccacttc ctgctctcga cccgcgccaa
      121 cttcagcgac ggcagcaacg ccctggtcac cgccgcgatc aacctcggct tcaccgccgc
      181 cgaggtcacc aacctacgca ccatcctgca gagccgcggc ttcaccgtga ccgcgtagtg
      241 ttgcgtcgat tcccttgtcg gcgcggccgg ccggaggcac gatggcagcg tgaagcacgg
      301 acccatcgtc ctcgcggcga tcggggcggc cggcgcgtct gcgggcacgg cgcgcgcgga
      361 atgtccgacg acaccggacg atgcggtgtg ccggccgtgg agcgccatgc tgctccccac
      421 cgggttcgcg gcggtgtatg cgccgcacgg cctggacgcc acgttctacg gcggcggcct
      481 ggaggcggtg ctcctggcgt ggaacgacaa ctccgacgcc tacggaccga gccagggccg
      541 gctgcggatc gatgtggcgt atctcacgag cagcaacgcg atgaccggcg gaatggcgat
      601 gtaccgcggc ggcgcgcagc tggcgttcga gcgcaacgca tcgcggtcgt acggcatccc
      661 gtacttcgcg gccgacctcg gcgggctgtg gagcgacgcg accgggcggc ggtggtacgt
      721 cgacggcggc gtcgggctct acctggtcca ccggcgcaac gtgatcgtcg acctcgaggt
      781 caccggcgtg ctgccgttcc gtgatccggg cacgctcggc ggcgtccggt cccggctcgg
      841 gctgtcgttc gcgctatggt gaagctcgtc gtcaacggca agccggtcga ggtcgacgtc
      901 gacgccgaca ccccgctcct gtgcagcacc cgcgctgcat gaactgccac ccgagcggcg
      961 acgcgccgct gcagtacgat gactcgcggc tgcacggcat ggcgatctcg cgccgctccg
     1021 aggccaacgg cgtcgcctgc gcgacctgcc accgcgaccg caacgacggg cgccccggcc
     1081 agccgcccgg cgcgcccagc tggcacctgc caccggcaga taccccgatg atcttccagg
     1141 gccgcacgcc gcaccagctg tgcctgcaac tgcttgaccc gcgccagacc cgcggccgcg
     1201 acgtcgccgc gctgatcgag cacgtcgccc acgacgagct ggtcggctgg ggctgggcgc
     1261 cgggggccgg ccgcaccccg gtcccggtcc cgcgcgccga cgtcgtcgcc gccatgcagg
     1321 cgtgggccga cgccggcacg ccctgtcccg agtaggcgct acgagctaag gtgccagcgg
     1381 cacggtcgtc gtgaccatcc actcgaacac cgggtcgaga ccatcgttgc ggtggaacgt
     1441 cagcttggcc gagtacagcg agttgacctt ggtcagcacg atcacgaggg ttgcctgtgg
     1501 catgttgctc gccccgtcct ggaacgtcag ggtcatgccg ccggtggtgg ccgtggccgt
     1561 cgcgagctgg tacatcgcga tgttcgccgg gatcggcggc gggttggtcg ccgtgcacac
     1621 gaacggcgtg gtcggggggc tcgacgggac gaccgcctcg acgttgaggt cctggacgtg
     1681 gcccgacgtc acctgccccg acggcaggat cgcgagctgg acgtcgagct gcctcagcgt
     1741 gatggtgtac ttgcagtaca tggcgccatc tgcggcagtg ccaccgaaca tcacgagcgg
     1801 cacatcgccg ggctgatttc gccacgtgac ggcgcccgct gccccatccg ggcggccgcc
     1861 atcgccgacc gccgcgtccg ccgggctgtt ggtgacggta ccgcacgcac ccacgagcat
     1921 cgcgacggcg agaagtctga acgccatgac gggatgttgg cgtcgcaccg ggcggccagc
     1981 aagcaaacct tgccggcaac cggtgaccgc gtcggcgtga cgatcgtcgc ggtctcactg
     2041 aatctgactg gagctcgcct agaattcgcc tagaactcga aatccacgcc ggcgaaccag
     2101 gtccggtcgc ccaccgacag cttctggtcc gagccgaagc cgtccatctt ggccagcgac
     2161 agctcggcgt agatcccggc gtgctggatc ccgctctgct gcatcgacat cgcggccgac
     2221 gcatcgatcc gctcggcgcg gatcgcgagg ccgatcgcgc cctgcaggcc gagcgacgcg
     2281 ccgagcgcct tgttctggga gcagttcggc tcgaggccgc cgtcgctgca cacccgcgcg
     2341 aagtggtcgt tgatcgagat ccaccacacg tagtagccga gcccggcgcg ggcgtacggc
     2401 accaccggga tgccgtagtt gtcgtcgagc acggtgaacc ggtaggtcgc ggtcagcgcg
     2461 agcgggacca gccgcatcgc gttgtgggcg ctggcatcgc gcatgctggt cggcgtcccg
     2521 tccggctgcg cgccgagcgc gcgcgcggtc ttctgccagt aaccgaccga gacgccgacg
     2581 ccgacctgcc cgaacccggt ccacaggaag cggtcgacgt cgagcatcgt catcatgtgc
     2641 tgggtggtgc cgaacatctg ctggaacggg ccggggtgca tgtcgagctg gttgtcgatg
     2701 tccggcgtgt acgggccgat ccggagcccc gcgtgccact cgaccaggct cggatcgccg
     2761 ggcggcacct gcgcctgctc ggcctgccgg gtcggatcgg tgtcctccca gtacggctgg
     2821 tagccgccgt tgccggcgtg cgcgcgcccc atgccgagca cgaagatcgc gccggtcgcc
     2881 gcga
//
LOCUS       DASVOR010001486        13775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_277288,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001486 DASVOR010000000
VERSION     DASVOR010001486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13775
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277288"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VF516_28080"
     CDS             complement(<1..309)
                     /locus_tag="VF516_28080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111634.1"
                     /translation="MDGGIALAEGGGRDERPPARVDVSGGLLELAVLPVAAGASAQDL
                     AAQQRRLVEPGQPGARTVAIDRVEPAERLVMAALVERDQRLDVFDRGYVGATAVAQVR
                     E"
     gene            337..2013
                     /locus_tag="VF516_28085"
     CDS             337..2013
                     /locus_tag="VF516_28085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111635.1"
                     /translation="MLIREQYGGGEVDPPFERGVAAAARSLAILPDRYEALALRARLQ
                     CDLAGYRGDRGEDVTDLLTRAVADAERAVEIVPARPEARLALAQIWRQWGEVRQGRSQ
                     DPAEQLGKALEIADAIAPADRGDEYYVLRGLAFKIWADYEDQIGRDAGRERGQAIDAY
                     TSALALDDGLAYVWFNLGINYHDRAAQPYNRDTDRDIQRAITAFDRARALDPSNIAVY
                     FYGGRLHYLQAKRRRDAGSDPGPDLALALARYREGLAIDARRPQLHSGIGTVLVEQAR
                     NAWDHGADPFPLFDAAQASYGRAAAVAPALGLGDNNLGWALAERAAYQRAHGEDPGPT
                     VRAAEAILRKATERAPSDAVPWSSLGYALTVLAGYELDQGRDPTASLAQARVALDRVM
                     ATAPQQALLYDGEAKGILARWRAHVGKGQADDFAAAAESYDRALASAPLDLAARVTYG
                     RFCRRWAAWLQDTRQDPGPALRRGLDLATEVLGKRPGWPDALVLRASLLLAQSESVTE
                     GAARRDLARRAAQDFRAALAVNPNLEPAWREPARDAEERGRATPWACCGH"
     gene            complement(1922..2380)
                     /locus_tag="VF516_28090"
     CDS             complement(1922..2380)
                     /locus_tag="VF516_28090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111636.1"
                     /translation="MPDPDITLHKTSNGKSFSPATYPKSGTVQRGDTITFKADQASGG
                     YVTFYAQSGLFYVPPGSNSGSENNGDWEFTAPAASSVDDLTLYVAANPATNTGYTISF
                     SPVSAADEPGGGVGDPSDGTINVRNKPTEWRDPAPQHHAPAPAMPARGSD"
     gene            complement(2593..4323)
                     /locus_tag="VF516_28095"
     CDS             complement(2593..4323)
                     /locus_tag="VF516_28095"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8111637.1"
                     /translation="MSVTLAPEEYGSTAIPPPRTTAGAATRLVPALTVAAHPDPRRVG
                     ERCLLTELLAGREIALSRTAPDFLPSGSALGTPLRDPFLSRRSIRFSPGAQGAIRLLT
                     ETGGSDVTLAEGPVRGEARFGPVALAAGVPLVLADRIVVVLHLVDLDLHDTGDSLQMI
                     GCSTGIERMRRAVRRLADLAVPVLLRGETGAGKERVARALHDHSPRRGGPFVSVNLGA
                     IPKDLAAAELFGATRGAYTGATRDREGFFAAARGGTLFLDEVGEAPPEVQAILLRVLE
                     TGEMYPIGSHKPVATDVRLIAATDANLEEHIRSGRFKAPLLHRLAGYEILLPPLRERR
                     EDIGLLFHHFAREELAAMGEAHRLSPADPRGEPWLPAALALRLVRYAWPGNIRQLRNI
                     TRQLVIYSRGQAQLQIDPRLAAELDAGASPLPVDAERLPVLEVERPVAVSERPLAAAA
                     ERPVDTTAVDVGRPPAQDAETRQKGRARRRPSDVTEEELRAALREAAWDLKAAGDRLG
                     VPRPSMYDLIERCPGIRTVGDLSAEEISRCFEACDGDLDRMVEHLQVSRRGLRRRVGE
                     LGLTHRRTAR"
     gene            complement(4573..5472)
                     /locus_tag="VF516_28100"
     CDS             complement(4573..5472)
                     /locus_tag="VF516_28100"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111638.1"
                     /translation="MASANRRTPEDTQRISRVAPADRELLAGRYEILSLLGSGGMAEV
                     FAGRDRLLERTVAVKRPRRDIGSDAEISERLQREAIALASIDSPHVVAIHDVGATPHG
                     VYLVMQRLFGRTLDEEIGAHGPVSASRACRIARDILAGLAAIHTSGLVHRDLKASNVL
                     LDRTDRAVLLDLGAALHLRRRSLTAPGTLLGTPECMAPEQLAQVPLDGRADLFQLGLI
                     LIYLVTGTMAGSAVDPTELAIPPALREVIARALAPADARYASAGEMRGALDQALAAST
                     LEPIEPVFSDRPTLRWPESSPRP"
     gene            complement(5597..6685)
                     /locus_tag="VF516_28105"
     CDS             complement(5597..6685)
                     /locus_tag="VF516_28105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111639.1"
                     /translation="MRRARAPRPADPQHILFALCDHYEPLWGKAPDDIGRRRVDVWAE
                     RYPALGEFRDSDGRPPRHGWFFPGEEYRPHFLDRLAELVRAGFGEVEFHLHHDGDTRH
                     TLAPRITEHLRTFAQHGHISRDPGRDPGCDRGHPGERFRWAFIHGNWSLANGRPDRKW
                     CGVDDELVLLHELGCYVDLTFPSAPDPCQPDKVNQIYWPTGDLTQRRAYEHGERARVG
                     AHHDDRLLMITGPLALAAKRDGLVPGIRIENGAITGDDPPTAARVQTWIDQAIHIAGR
                     PEWVFVKVHTHGAIEATAASLLGDGGRALHQALRDQARDRRAQLHYVTAREMYNVARA
                     AMAGETGNPNVYFDYVIPPPPIATAKPR"
     gene            6781..8007
                     /locus_tag="VF516_28110"
     CDS             6781..8007
                     /locus_tag="VF516_28110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111640.1"
                     /translation="MDLYRPLLEKVLFPAFETARGRPTVPLLQFLRGSERWSADALRD
                     LQAGLLRRLIRHARAHTTYYRKLLGHRGLVPEDFATVDDLRHLPVLDRELARETLEAR
                     TAGVPRWVIKKSAGGMTDAPVVVKYNAESRHWRDATRWRGYGWAGYRIGMRVLRYWGD
                     PPSSEGWLARGKQALDRTLKRSLHIDCIVRSERALAGTARRLREFQPQVIVAYAAGAA
                     ALARYVNDNGLRTWADIPVIVGAERLWPHDRNQIHQAFGPAFETYGCGEVMMIAAECE
                     AHDGMHTAMENLIVEVIVRAPDGAVRAARPGEVGEVAITDLHNLSCPMIRYVTGDLAI
                     ARAESRCACGRGLASIDPIDGRVTETLPGTLTDAEAPAVGGVAGEIPLTSAGPRKVRH
                     RRAGRDQRRHVSVTLP"
     gene            8004..9380
                     /locus_tag="VF516_28115"
     CDS             8004..9380
                     /locus_tag="VF516_28115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111641.1"
                     /translation="MIDLYGPLLAKALFPAFEAARGRPTVKLLESLRETERWTPGALR
                     DLQVGMLRRVLRHAYRHTGFYRRMLDERGLAPEDFRAVDDLGKLPLLERPIVRASIDA
                     RTAAVPPLPVITKATSGSSGEPVVVRYNAESRHWRDAIRWRGYGWGGYRIGMRALHYW
                     GAGPKIATWWKQKKVQVDHLVKRDLYVDCTPRGDDALQGVVAQIRRFRPHAMVAYANG
                     AGALARFVNDRGLRDWGPIPVLTGAERLLPHDRAAIEQAFGPAFDTYGCREVMMIGSE
                     CEAHDGMHTAMENLIVELIVRQPDGAIRAARPGEPGEVVVTDLHNLACPMIRYVTGDV
                     AVARGDARCACGRGLVRIGPIEGRVTETLTDGQGRPVSGLVFNIVLGVIGGFAKSFQV
                     VQRRDRSVVFKVVPFQGDVVPPREAKIIRDYVEKYLPGVPVTIEVVRDIPPTAAGKRH
                     PVVVERPA"
     gene            9425..10423
                     /locus_tag="VF516_28120"
     CDS             9425..10423
                     /locus_tag="VF516_28120"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8111642.1"
                     /translation="MTVPLVLVTGFLGAGKTTLINRLLVRRQARQATGKLGVIVNELG
                     EVGIDGALLGGQAARQIELPGGCVCCVLGDQLDTTLLELVAQNPGLEAIVLETTGVAE
                     PLPIAWAIAREPVAQQVRLAAVVTLIDATHFLAARPVSASVDAQVAYADVLLVTKAEV
                     GGSDADRAESVARELAPRALVRRGTADEHAAWLEALLADPVLPDRPSATPLAPLSQPP
                     SELGPHVHDADCRHLDSPAAHGIDSVWLAIDHPVDVEELADQLAELPPNYVRIKGIVQ
                     DGETWVAVHRVGPRVSSEPIALDEAPGAPQQARIVALGPGVTVTDLARCVAAAAHG"
     gene            10481..11581
                     /locus_tag="VF516_28125"
     CDS             10481..11581
                     /locus_tag="VF516_28125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111643.1"
                     /translation="MTHRRFVLALAVVALAGAACGQDPIEVRPSQNGDYNHAALLGAV
                     DRFVAAGRTPDAYAELSRTVLALRAGMDRSVAREAELKLLVLALAPVQSVQARPMPAQ
                     VDALATTVWPTLLAPEIEADDLLDKRDPRAAEWIAQPREDARSYLIRICSGALAADCK
                     RVVPELQGPVVAAIATRRATERVRNAIADCVMCSAEPGWHEAARSWEALDRMLSASRA
                     DIERRADPDNWPVAGVAAEPDPQLPEAELDPTGDVVIGGQHYGAAERIAALRDLRGPN
                     PAIALHLRPETSLAQVRGVLADAYKAGATRVAVIARGDRYPWPRKIYWIAGGTGSGTG
                     YRAGLRPTDSLQLLLHATDVVAAAPGTVARVD"
     gene            complement(11716..12009)
                     /locus_tag="VF516_28130"
     CDS             complement(11716..12009)
                     /locus_tag="VF516_28130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111644.1"
                     /translation="MSKPVSPSRLSLRSGAVLFSLLASLGACATLDTPRRGFAEASAV
                     QPSQLASWGFATIAQPSQPTLHGYASVPEPVQPAVWGYAELPAQQASVTQASK"
     gene            complement(12221..13588)
                     /locus_tag="VF516_28135"
     CDS             complement(12221..13588)
                     /locus_tag="VF516_28135"
                     /inference="COORDINATES: protein motif:HMM:NF022376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BREX system ATP-binding domain-containing
                     protein"
                     /protein_id="HEX8111645.1"
                     /translation="MTRTTPPRPPPRPTTPGELDRISARRILAAMGENGKPPELGIAH
                     VNAGNESYLRVIDSAYIQDLIIAVEGSSFKLVQGTYGAGKTHFLYCVRDLAWRRRLLT
                     AMVTISPKECPFNRPLAVYRAIAQRIERPRTAAGDEVRGIDDLIRHAIEQRITDDGRD
                     GTGAWIDATLARTAFTRHSFRDAVVGFARAIIDRDEPAARRLAAWLRGEDITLAEAKH
                     AGLYEVPGNDNGFGMLRSLVQLVPRLGWQGSVLLLDEAERRLSAGEKPTKALGETVDH
                     LRELIDLCGRSELPRTLILYAVTPMFTEQVLPLYPALQQRLGAPIQFLGPHNPKAPVI
                     DLEALDLEPKRLLLEVGKRLAAVACLAYDWSPTDDLIDDNLRRLAAIVTAEQLEVGHR
                     RFFVRLWVRLLDQLRLGTTRVLGDDELRSLVRDEQALIAEEVDDGMVNTFFGIPFLAK
                     PPKRR"
     gene            complement(13585..>13775)
                     /locus_tag="VF516_28140"
     CDS             complement(13585..>13775)
                     /locus_tag="VF516_28140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111646.1"
                     /translation="FAWDLARLRRERRLVVGGRRIDLGVATGHAAGRRSQVVWIENDG
                     GGGCFFQSFRLIAQEVRT"
BASE COUNT         1892 a         4942 c         5033 g         1908 t
ORIGIN      
        1 ctcgcgtacc tgcgcgacag ccgtggcgcc gacgtaccct cggtcgaata cgtcgaggcg
       61 ctggtcgcgt tctacgaggg ccgccatgac gaggcgctcg gccggctcga cgcgatcgat
      121 cgcgacggtg cgggcgccgg gctgacctgg ttctacgagg cgccgctgct gcgcggcgag
      181 atcctgcgcg ctcgcgccgg ccgccactgg cagaacggcg agctcgagca ggccgccgct
      241 gacgtcgacg cgagccggcg ggcgttcgtc acggccgcca ccctcggcga gagcgatccc
      301 gccgtccacg aggcgctggg cgatctcgag tccagcgtgc tgatccgcga gcagtatggc
      361 ggcggcgagg tcgatccacc gttcgaacgc ggcgtcgcgg cagccgcgcg ctcgctcgcc
      421 atcctgcctg accgctacga ggccctggcg ctgcgggcgc ggctccagtg cgacctggcc
      481 gggtaccgcg gcgaccgggg cgaggacgtg acggacctgc tcacccgcgc ggtcgccgac
      541 gcagagcgcg ccgtcgagat cgtgccggcg cgacccgaag cgcggctggc gctggcgcag
      601 atctggcggc aatggggcga ggtccggcag ggccggagcc aggatcccgc cgagcagctc
      661 ggcaaggcgc tcgagatcgc ggacgccatc gcgcctgctg accgcggtga cgagtactac
      721 gtcctccgcg gactcgcatt caagatctgg gcggattacg aggaccagat cggacgggac
      781 gcggggcgcg agcgcggcca ggcgatcgac gcctatacga gcgcgctcgc gctcgatgac
      841 ggactggcct atgtctggtt caatctcggc atcaactacc acgaccgcgc cgcgcagccc
      901 tataaccgag ataccgatcg agatatccag cgagcgatca ccgcgttcga ccgggctcgc
      961 gcgctcgatc cgagcaacat cgccgtgtat ttctatggtg gcaggctcca ctacctccag
     1021 gccaaacggc ggcgggacgc gggtagcgat cccggccccg atctggcgct cgcgctggcc
     1081 cgttaccgcg aagggctcgc catcgatgcc agacgcccac aactccacag cgggatcggc
     1141 acggtgctcg tcgaacaggc gcggaacgcg tgggaccatg gcgcggatcc cttcccgttg
     1201 ttcgatgccg cacaggcctc ctacgggcga gctgccgccg ttgccccggc gctcgggctc
     1261 ggcgacaaca acctcggctg ggcgctggcc gagcgcgcgg cgtatcagcg tgcgcacggc
     1321 gaggatccag ggccgacggt ccgcgcggcg gaggcgatcc tgcgcaaggc gaccgagcgc
     1381 gcgcccagcg acgccgtccc ctggtccagc ctgggctatg cgctgaccgt cctcgccgga
     1441 tacgagctcg accagggtcg ggatccaacc gcgagcctgg cgcaagcgcg ggtggcgctc
     1501 gatcgggtga tggccacggc cccgcagcaa gccttgctct acgatggcga agcgaagggg
     1561 atcctggcgc gctggcgggc ccacgtgggc aaggggcagg ccgatgactt cgccgccgca
     1621 gcggagtcct acgaccgggc gctcgcgagc gcgccactgg acctggcggc gcgggtcacg
     1681 tacggcaggt tctgtcgccg atgggcggcg tggctgcagg acacccggca ggatccgggt
     1741 cctgctctgc gccgcggcct cgatctggcc acggaggtgc tcgggaagcg tcccgggtgg
     1801 cccgatgcgc tggtgctgcg cgcgagcctg ctcctcgcgc agtccgagag tgtgaccgag
     1861 ggagctgcgc gccgggatct tgcgcgtcgc gccgcccagg acttccgcgc tgcgctcgcg
     1921 gtcaatccga acctcgagcc ggcatggcgg gagccggcgc gtgatgctga ggagcggggt
     1981 cgcgccactc cgtgggcttg ttgcggacat tgatcgtgcc gtcgctcggg tcacccactc
     2041 cccccccggg ttcgtcagcg gcggaaactg ggctgaacga gatcgtgtat ccggtgtttg
     2101 tggcggggtt ggccgcgacg tagagagtga gatcgtctac ggaagatgca gcaggcgccg
     2161 tgaattccca gtcgccattg ttttcggatc cgctgttcga tccgggcggc acgtagaaca
     2221 agccgctctg ggcgtagaac gtgacatagc ccccggacgc ctggtcggcc ttgaacgtga
     2281 tggtatcgcc ccgctgcacc gtgccactct ttggataggt cgccggggag aaggacttac
     2341 cgttgctggt cttgtgaagc gtgatatccg gatcgggcat tgcgaatctc ctgtcttgta
     2401 ggaatgtcct ggaagctgcc tgcggtcgtc gaaccctgaa gcaactcagg taccagcccc
     2461 ggcgctgtcg aagtcgggag ctgcacagcc gctggcgccc ggatctcgcc attcgacgcg
     2521 tcgaaccggc cgccggagca tcgagaaggc tgcgtggacg ggtcgagggc tgtcaccgac
     2581 gcgtcaagtg gctcaccgcg ccgtcctgcg gtgcgtgagg cccagctcgc cgacacgccg
     2641 gcggagaccc cggcgtgaca cctggaggtg ctcgaccatc cggtcgagat ccccgtcgca
     2701 cgcctcgaag cagcgggaga tctcctccgc gctgaggtct ccgacggtgc gaatccccgg
     2761 gcaccgctcg atgaggtcat acatcgacgg ccgggggaca ccgagccgat cgccggctgc
     2821 cttgaggtcc caggctgcct cgcgcagcgc ggcgcgcagc tcctcctcgg tcacgtccga
     2881 aggtcggcga cgcgcccggc ccttctgccg ggtctcggcg tcctgcgccg gcggtcgtcc
     2941 cacatccacc gcagtcgtat ccaccggtcg ttccgcggcc gccgccagcg gtcgctctga
     3001 caccgccacc ggtcgttcca cctccagcac cggcagtcgt tccgcgtcca ccggcagcgg
     3061 cgaggcgccc gcatcgagct cggcggcgag ccgcggatcg atctgcagct gggcttgccc
     3121 gcggctgtag atgacaagct gccgggtgat gttgcggagc tggcggatgt tccccggcca
     3181 ggcatagcgc acgaggcgca gggcgagcgc cgccggcagc cacggctcac cgcgcggatc
     3241 cgccggcgag aggcggtgcg cttcccccat cgccgccagc tcctcccggg cgaagtggtg
     3301 aaagagcaag ccaatgtcct cgcggcgctc gcgcagcggc ggcaagagga tctcgtagcc
     3361 ggccaggcgg tgcaggagcg gcgccttgaa ccgaccgctg cggatgtgct cctcgaggtt
     3421 ggcgtcggtc gcggcgatca ggcgcacgtc cgtggcaacg ggcttgtggc tgccgatcgg
     3481 gtacatctcg ccggtctcga gcacgcgcaa caggatcgcc tgcacctcgg gcggcgcctc
     3541 gcccacctcg tcgaggaaca gcgtgccgcc gcgggcggcg gcgaagaacc cctcgcgatc
     3601 acgcgtcgcg ccggtgtagg cgcccctggt cgcgccgaac agctcggcgg cggcgaggtc
     3661 cttggggatg gcaccgaggt tcacgctgac gaacggaccg ccccgccgcg ggctgtgatc
     3721 gtgaagcgcc cgcgcgaccc gctccttgcc cgcgccggtc tcgcctcgga gcaggaccgg
     3781 gacggcgaga tcggcgagcc gccggaccgc ccgccgcatg cgctcgatgc ccgtgctgca
     3841 gccgatcatc tggagggagt cgccggtgtc gtggaggtcg aggtcgacga ggtgcaggac
     3901 gacgacgatg cgatcggcga gcacgagggg gaccccggcg gcgagggcga cgggcccgaa
     3961 gcgagcttcg ccgcgcaccg gcccttctgc cagggtgaca tcgctcccgc ccgtctcggt
     4021 caggagccgg atcgcgccct gagcgccggg cgagaagcgg atcgaccggc ggctgaggaa
     4081 cggatctctc aggggtgtac cgagggcgct gcccgacggc aggaagtcag gcgcggtgcg
     4141 tgacagggcg atctcgcgac cggccagcag ctccgtgagc aagcaccgct cgcccacccg
     4201 gcggggatcg ggatgggcag cgacggtcag cgctggaacg aggcgcgtcg cagcgccagc
     4261 cgtggttcgc ggcggcggga tcgcagtgga gccgtactcc tccggcgcca gggtcacgga
     4321 catctcgcag cggatcatat acgcgcgatg gacgtgtccc ggaatcctgg agaacctgcc
     4381 agtccaggca cgcgctcatc ggtgcggcca gtgggagcac cgcgctgtga cacgcacaag
     4441 gttggtcact ctccgtgtcg ggcccctacg acaagcgggt ttgcggaacg cgtctccggg
     4501 ccatgggaag gccccggatc gatcagaagg aagcttgcgc tggcccgagc agactcgcag
     4561 ggggtacgcg agtcatggcc gtggggagga ctcgggccag cgcaaggtag gtcggtcgga
     4621 gaacaccggc tcgatcggct cgagcgtgga cgcggccagc gcctgatcga gcgcgccgcg
     4681 catctcgccg gccgatgcgt agcgcgcatc ggccggggcc agcgcccgcg cgatcacctc
     4741 gcgcagcgcc ggcgggatgg cgagctcggt cggatcgacc gcgctgcccg ccatcgtgcc
     4801 ggtgaccagg tagatcagga tcagcccgag ctggaacagg tcggcgcggc cgtcgagcgg
     4861 gacctgcgcg agctgctcgg gcgccatgca ctccggcgtg ccgagcagcg tgccgggcgc
     4921 ggtcagcgac cgccggcgca ggtggagcgc cgcgccgagg tcgagcagca ccgcgcggtc
     4981 tgtgcggtcg agcaggacgt tggacgcctt gaggtcgcgg tggaccagcc cgctggtgtg
     5041 gatcgcggcg agcccggcga ggatgtcgcg cgcgatccgg cacgcacgcg acgccgacac
     5101 cgggccgtgc gcgccgatct cctcgtcgag cgtgcggccg aacagccgct gcatcaccag
     5161 gtacacgccg tgcggcgtgg cgccgacgtc gtggatcgcg acgacgtgcg gcgagtcgat
     5221 cgacgccagc gcgatcgcct cgcgctgcag ccgctcggag atctcggcgt cgctgccgat
     5281 gtcgcggcgc ggccgcttga ccgcgaccgt ccgctcgagc aggcgatcgc ggccggcgaa
     5341 cacctcggcc atgccgccgc tgccgagcag cgacaggatc tcgtagcggc cggcgagcag
     5401 ctcgcggtcg gcgggcgcca cgcgcgagat gcgctgcgtg tcctccggcg ttcgccggtt
     5461 cgcgctggcc acggcccgca tcgagttccc cgggacacag gctacgtcga accctcgatc
     5521 gtcctcgcgg actcaccact gcctcacgac ggacgcggcg gttttgtcgt cgcgtgtggg
     5581 cggtcctgcc aggtttctac cggggcttcg cagtcgcgat cggtggcggt ggaattacgt
     5641 agtcgaagta cacgttgggg ttgccggtct cgccggccat cgcggcgcgc gcgacgttgt
     5701 acatctcgcg ggcggtcacg tagtgcagct gcgcacggcg gtcgcgtgcc tgatcgcgca
     5761 gcgcctggtg cagcgcgcgg ccgccgtcgc cgagcagcga cgccgcggtg gcctcgatcg
     5821 cgccgtgggt gtggaccttg acgaacaccc actcgggccg gccggcgatg tggatcgcct
     5881 ggtcgatcca ggtctggacc cgcgccgcgg tcggcggatc gtcgccggtg atcgcgccgt
     5941 tctcgatccg gatcccgggc accaggccat cgcgcttggc ggccagcgcg agcggcccgg
     6001 tgatcatcag cagccggtcg tcgtgatggg cgccgacgcg cgcgcgctca ccgtgctcgt
     6061 acgcacggcg ctgggtgagg tcgccggtcg gccagtagat ctggttgacc ttgtcgggct
     6121 ggcacgggtc gggcgccgac ggaaacgtca gatcgacgta gcagccgagc tcgtggagca
     6181 ggaccagctc gtcgtcgacc ccgcaccact tgcggtccgg ccgcccgttg gccaggctcc
     6241 agttcccgtg gatgaacgcc cagcggaacc gctcgccggg gtgacctcgg tcgcacccgg
     6301 ggtcgcgccc ggggtcacgg gagatgtggc cgtgctgtgc gaacgtgcgc aggtgctcgg
     6361 tgatgcgcgg tgccagcgtg tgacgggtat ctccgtcgtg gtgcaggtga aactcgacct
     6421 ccccgaaccc ggcgcgcacc agctcggcga gccgatcgag gaagtgcggg cggtactcct
     6481 cgcccgggaa gaaccagccg tggcgcggcg gccggccgtc gctgtcgcgg aactcgccaa
     6541 gcgccgggta gcgctcggcc cacacgtcga cgcggcgccg gccgatgtcg tcgggggcct
     6601 tgccccacag cggctcgtag tgatcgcaca gcgcgaacag gatgtgttgc ggatccgccg
     6661 ggcgtggcgc gcgcgcacgg cgcacgatgt gtcttccgta gtcgcgaatc cagtaatgaa
     6721 ggttcttttt gcggagtcgg cctagcacgc tgactgcatg gtagccggat cggtgcgccc
     6781 atggatctct accggccgct gctcgaaaaa gttttgttcc ccgcgttcga gaccgcgcgc
     6841 gggcggccga ccgtgccgct gctccagttc ctgcgcggca gcgagcgctg gtcggccgat
     6901 gcgctgcgcg atctgcaagc gggcctgttg cgccgcctga tccgccacgc tcgggcccac
     6961 acgacgtatt accgcaagct gctcggtcat cgcggcctcg tgcccgagga cttcgcgacg
     7021 gtcgacgacc tccgtcacct cccggtgctc gaccgcgagc tcgcgcgcga gaccctggag
     7081 gcgcggaccg ccggggtgcc gcgctgggtg atcaagaagt cggccggcgg catgaccgac
     7141 gcgccggtcg tcgtcaagta caacgccgag tcgcggcact ggcgcgacgc cacgcgctgg
     7201 cgcggctatg gctgggcggg ctaccggatc ggcatgcgcg tgctgcgcta ctggggcgat
     7261 ccgccgtcat ccgagggctg gctggcgcgc ggcaagcagg cgctcgaccg cacgctcaag
     7321 cgcagcctcc acatcgactg catcgtgcgc agcgagcgtg cgctggcggg caccgcgcgc
     7381 cggctgcgcg agttccagcc ccaggtgatc gtcgcgtacg cagcgggcgc cgccgcgctc
     7441 gcccgctacg tcaacgacaa cggcctgcgg acctgggccg acatcccggt gatcgtcggc
     7501 gccgagcggc tgtggccgca cgaccgcaac cagatccacc aggcgttcgg ccccgcgttc
     7561 gagacctacg gctgcggcga ggtcatgatg atcgccgccg agtgcgaggc ccacgatggc
     7621 atgcacaccg cgatggagaa cctgatcgtc gaggtcatcg tgcgcgcgcc cgacggcgcg
     7681 gtccgcgcgg cgcggcccgg cgaggtcggc gaggtcgcga tcaccgatct ccacaacctg
     7741 tcgtgcccga tgatccgcta cgtcaccggc gacctcgcga tcgcccgggc cgagagccgg
     7801 tgcgcgtgcg ggcgcggcct ggcctcgatc gacccgatcg acggccgggt caccgagacg
     7861 ctgccgggga cgctgaccga cgccgaggcc cccgcggtcg gcggcgttgc cggtgagatt
     7921 ccgctgactt cggccggccc acgcaaggtc cggcaccgtc gagccggccg cgaccagcgc
     7981 cgccatgtta gcgtgaccct gccgtgatcg acctgtacgg gccgctcctg gccaaggcgc
     8041 tgttccccgc gttcgaggca gcccgcggcc gtcccaccgt caagctcctg gagtcgctgc
     8101 gcgagaccga gcgctggacg ccgggcgcgc tgcgcgatct ccaggtcggg atgctgcgcc
     8161 gcgtgctgcg ccacgcctac cgccacaccg ggttctaccg ccggatgctc gacgagcgcg
     8221 gcctggctcc cgaggacttc cgcgcggtcg acgacctggg caagctcccg ctgctcgagc
     8281 gcccgatcgt gcgggcgagc atcgacgcgc gcaccgccgc cgtcccgccg ctgccggtga
     8341 tcaccaaggc gaccagcggc agctcgggcg agccggtggt cgtgcgctac aacgccgagt
     8401 cgcggcactg gcgcgacgcg atccgctggc gcggctacgg ctggggcggc taccggatcg
     8461 ggatgcgcgc gctgcactac tggggcgccg ggcccaagat cgccacgtgg tggaagcaga
     8521 agaaggtcca ggtcgatcac ctggtcaagc gcgacctcta cgtcgactgc acgccgcgcg
     8581 gcgacgatgc gctgcagggc gtcgtcgccc agatccgccg gttccggccc cacgccatgg
     8641 tcgcgtacgc caacggcgcc ggcgcgctcg cccggttcgt caacgaccgc ggcctgcgcg
     8701 actggggccc gatcccggtg ctgaccggcg ccgagcgcct gctgccccac gaccgcgccg
     8761 cgatcgagca ggcgttcggc cccgcgttcg acacctacgg ctgccgcgag gtcatgatga
     8821 tcggctcgga gtgcgaggcc cacgacggca tgcacaccgc gatggagaac ctgatcgtcg
     8881 agctgatcgt ccgccagccg gacggtgcga tccgcgcggc gcggcccggc gagcccggcg
     8941 aggtcgtggt caccgacctc cacaacctgg cgtgcccgat gatccgctac gtcaccggcg
     9001 acgtcgcggt cgcgcgcggc gacgcgcgct gcgcgtgcgg ccgcggcctg gtccggatcg
     9061 gcccgatcga gggccgggtc accgagaccc tgaccgacgg ccagggccgg ccggtcagcg
     9121 gcctggtgtt caacatcgtg ctcggcgtga tcggcgggtt cgcgaagagc ttccaggtcg
     9181 tgcagcgccg cgaccggagc gtcgtgttca aggtcgtgcc gttccagggc gacgtggtgc
     9241 cgccgcgcga ggccaagatc atccgcgact acgtcgagaa gtacctgccg ggcgtgccgg
     9301 tcaccatcga ggtcgtccgc gacatcccgc cgaccgccgc cggcaagcgc catcccgtcg
     9361 tggtcgagcg acctgcctga tcggcgcgcc gccgccgcgg acggtccgcg gtaggcttcc
     9421 gcgggtgacc gtcccgctcg tcctcgtcac cggcttcctc ggcgccggca agaccacgct
     9481 gatcaaccgc ctgctcgtgc ggcgccaggc gcgccaggcg accggcaagc tcggcgtgat
     9541 cgtcaacgag ctcggcgagg tcggcatcga cggcgcgctg ctcggcggcc aggccgcgcg
     9601 ccagatcgag ctgccgggcg gctgcgtgtg ctgcgtgctc ggcgatcagc tcgacaccac
     9661 gctgctcgag ctggtcgcgc agaacccggg gctcgaggcg atcgtgctcg agaccaccgg
     9721 cgtggccgag ccgctgccga tcgcgtgggc gatcgcgcgc gagccggttg cccagcaggt
     9781 ccgcctcgcc gcggtcgtga ccctgatcga cgccacccac ttcctcgccg cgcggccggt
     9841 gtcggcctcg gtcgatgccc aggtcgccta cgccgacgtc ctgctggtga ccaaggcgga
     9901 ggtcggcggt tccgacgccg accgtgcgga atctgtcgcc cgcgagctcg ccccgcgcgc
     9961 gctggtccgc cgcggcaccg ccgacgagca cgccgcatgg ctcgaggccc tgctcgccga
    10021 tccggtgctg cccgaccgcc cctcggccac cccgcttgct ccgctttccc agccgccgag
    10081 cgagcttggc ccgcatgtgc acgacgcgga ctgtcgtcat ctcgacagcc cggctgcaca
    10141 tggaatcgac agcgtgtggc tcgcgatcga ccacccggtc gacgtcgagg agctggccga
    10201 ccagctcgcc gagctccctc ccaactacgt gaggatcaag gggatcgtcc aggacggcga
    10261 gacctgggtc gcggtgcacc gcgtcggtcc ccgcgtgtcg agcgagccca tcgcgctcga
    10321 tgaggccccg ggcgctcccc agcaagcacg catcgttgcg ctcggcccgg gggtcacggt
    10381 gacggatctc gcgcgctgtg tcgcggcggc ggcgcacggc tgacacagaa gcccggccct
    10441 ccgcgggcat ccgccttgct tccccacccg tacagccaac atgacacacc gtcggttcgt
    10501 cctcgcgctg gccgtggtcg ctctcgccgg cgcggcctgc ggtcaggacc cgatcgaagt
    10561 ccgcccgagc cagaacggcg actacaacca cgcggcgctg ctcggcgccg tcgacaggtt
    10621 cgtcgccgcc ggccgcacgc ccgacgcgta cgccgagctg tcgcggaccg tgctcgcgct
    10681 gcgcgccggc atggatcgct ccgtcgcccg cgaggccgag ctcaagctcc tcgtcctcgc
    10741 gctggcgccg gtgcagtcgg tccaggcccg gccgatgccg gcgcaggtcg acgccctcgc
    10801 caccacggtg tggcccaccc tgctcgcccc cgagatcgag gccgacgatc tgctcgacaa
    10861 gcgcgacccc agggccgccg agtggatcgc gcagccccgc gaggacgcgc gcagctacct
    10921 gatccggatc tgcagcggcg cgctggcggc ggactgcaag cgcgtcgtcc ccgagctcca
    10981 gggtccggtc gtcgccgcca tcgccacccg gcgcgccacc gagcgcgtgc gcaacgccat
    11041 cgccgactgc gtgatgtgca gcgccgagcc cggctggcac gaggccgccc ggtcctggga
    11101 ggcgctcgac cgcatgctca gcgcctcgcg cgccgacatc gagcgccgcg ccgaccccga
    11161 caactggccg gtcgccggcg tcgccgccga gcccgatccg cagctccccg aggccgagct
    11221 cgatccgacc ggcgacgtcg tgatcggcgg ccagcactac ggcgccgccg agcgcatcgc
    11281 cgcgctccgc gacctccgcg gcccgaatcc ggcgatcgcg ctccacctcc gccccgagac
    11341 ctcgctcgcc caggtccgcg gcgtgctcgc cgacgcgtac aaggccggcg ccacccgcgt
    11401 cgccgtcatc gcccgcggcg accgctatcc ctggccgcgc aagatctact ggatcgccgg
    11461 cggcacaggc tccggcaccg gctaccgcgc cggcctgcgc cccaccgact cgctccagct
    11521 cctgctccac gccaccgacg tcgtcgccgc cgcgccgggc acggtcgccc gcgtcgactg
    11581 aacgtccgga tcccggacgc caccggcgcc cggtacgccg ccaggatcca cggcgctgcg
    11641 cccgccgcct ggccgagctt tccgggctcg acccgacggc gatcgccctg cagcaggcgc
    11701 cccgggcgcc gctggttact tcgacgcctg ggtgaccgat gcctgctgcg ccggcagctc
    11761 ggcgtagccc cagaccgcag gctgcaccgg ctccgggacg ctggcgtagc cgtgcagcgt
    11821 cggctgcgac ggctgggcga tcgtggcgaa gccccacgac gccagctgcg acggctgcac
    11881 cgccgatgcc tcggcgaacc cgcgccgcgg cgtatcgagc gtggcgcacg ccccgagcga
    11941 ggcgagcaac gagaacagga ccgcgccgga gcgcagcgac aagcgtgacg ggctgactgg
    12001 tttcgacatg acggacctct gcgccgacgc tgtgcaacgc gctcgccagc ccgccgcccg
    12061 tgccgagccg cagacatgcg cgatcggcgc cgccgggctc tcggcaattg tctgtcggcc
    12121 cgacggcggc tggcttgcgc atcgacacgc ccgatcaggc agggtcatgc caggcgatgg
    12181 gagggggacc agcggcttgg gtggccgcgg cacggcgcgg tcaccgccgc ttcggcggct
    12241 tggcgaggaa cgggatgccg aagaaggtgt tgaccatgcc gtcgtcgacc tcctcggcga
    12301 tcagggcctg ctcgtcgcgg accaggctgc gcagctcgtc gtcgccgagc acccgggtgg
    12361 tgccgaggcg gagctgatcg agcaggcgga cccagagccg gacgaagaac cggcggtggc
    12421 cgacctcgag ctgctcggcg gtgacgatcg cggcgagccg gcgcaggttg tcgtcgatca
    12481 ggtcgtcggt gggcgaccag tcgtaggcga ggcaggcgac cgcggcgagg cgcttgccga
    12541 cctcgagcag gagccgcttg ggctcgaggt cgagggcctc gaggtcgatc accggggcct
    12601 tggggttgtg cggcccgagg aactggatcg gtgcgccgag ccgctgctgc agggcggggt
    12661 agagcggcag cacctgctcg gtgaacatcg gggtgacggc gtagaggatc agggtgcgcg
    12721 gcagctcgga ccggccgcac aggtcgatca gctcgcgcag gtgatcgacg gtctcgccca
    12781 gcgccttggt cggcttctcg ccggcggaca gccggcgctc ggcctcgtcg agcaggagga
    12841 ccgagccctg ccagccgagc cgcgggacga gctggaccag cgagcgcagc atgccgaagc
    12901 cgttgtcgtt gccggggacc tcgtagagcc cggcgtgctt ggcctcggcg agcgtgatgt
    12961 cctcgccgcg cagccaggcg gcgagccggc gggcggcggg ctcgtcgcgg tcgatgatgg
    13021 cgcgggcgaa gccgacgacg gcgtcgcgga acgagtggcg ggtgaacgcg gtgcgggcga
    13081 gcgtggcgtc gatccaggcg cctgtgccgt cgcggccgtc gtcggtgatg cgctgctcga
    13141 tcgcgtggcg gatcaggtcg tcgatgccgc ggacctcgtc gccggctgcc gtgcgcggcc
    13201 gctcgatccg ctgggcgatg gcgcggtaaa ccgcgagcgg acggttgaac gggcactcct
    13261 tgggcgagat cgtgaccatg gcggtgagca ggcggcgccg ccacgcgagg tcgcgcacgc
    13321 agtacaggaa gtgggtcttg ccggcgccgt aggtgccctg gaccagcttg aagctcgagc
    13381 cctcgacggc gatgatcaga tcctggatgt acgcgctgtc gatcacgcgg aggtaggact
    13441 cgttgccggc gttgacgtgg gcgatgccga gctcgggcgg cttgccgttc tcgcccatcg
    13501 ccgcgaggat gcggcgcgcg ctgatccggt cgagctcgcc gggggtcgtg ggcctggggg
    13561 gcggcctggg cggcgtggtt cgggtcacgt gcgtacctcc tgggcgatca gccggaacga
    13621 ctggaagaag cagccaccgc cgccatcgtt ctcgatccac acgacctggg accggcggcc
    13681 cgcggcgtgg ccggtcgcga cgccgagatc gatccggcgg ccgccgacga ccagccggcg
    13741 ctcgcggcgc agccgcgcga ggtcccacgc gaact
//
LOCUS       DASVOR010001487         2685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_44657,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001487 DASVOR010000000
VERSION     DASVOR010001487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2685
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44657"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VF516_28145"
     CDS             <1..634
                     /locus_tag="VF516_28145"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8111647.1"
                     /translation="ADPTSRPLDDEFVRHFYAGTTSIEAADDKGWVVSVTPSGGWVPA
                     VIAGKTGIGLSQRMQSFVLDPAENPFNVVEPGKHPRVTLTPTLALKDGKPYLSFAVQG
                     GDSQDQNLLQFFLNVVEFGMNVQEACEAPNINSYQMRSSFGAHDARPGKMLINEQLPA
                     WVRGELGKMGYSFEVKPRTTGPINAIFFDQPHGTFWGGSSDDGDDYGIAW"
     gene            complement(719..1201)
                     /locus_tag="VF516_28150"
     CDS             complement(719..1201)
                     /locus_tag="VF516_28150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111648.1"
                     /translation="MTLTPGDIQVLARHPRTRSLRPLVAGDIEPVLQALPAHVPDPIR
                     WWITEFGVIDIERPIDSRPHLLLLGLSPSSTIDDDIMAFNRSNPTLPPGYLVFAHEFD
                     GELDHVYAIDASGAVVRSIGFLFPSLEYKEEYATSTWEAASTDLEAFWRSKVRSYLED
                     "
     gene            complement(1218..1469)
                     /locus_tag="VF516_28155"
     CDS             complement(1218..1469)
                     /locus_tag="VF516_28155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111649.1"
                     /translation="MTPPRCKCAVGGERMWHKPDFGISYANYEYSFPVTPRASPSGDM
                     FGGTFAETNPTGRNLDTAGWSVKILESVAPGEPSTPGSE"
     gene            complement(1515..2027)
                     /locus_tag="VF516_28160"
     CDS             complement(1515..2027)
                     /locus_tag="VF516_28160"
                     /inference="COORDINATES: protein motif:HMM:NF020904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042546 - cell wall biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEX8111650.1"
                     /translation="MARRGYAVTVDAVRRFERELGLEIPDDYRRFLLEVNGGRTARSH
                     RVFIVQQRAGRRRDETVLNSLFSLDDSDDSRDLATAQKPYNPDVKLPDGLLEIGYDGM
                     GGRIVLSLVGPHRGEVWYLDTGDPRPTDANPRVEWFDRRDVWKIAGSFAEFMASLRPL
                     DDDLGASGSP"
     gene            complement(2038..>2685)
                     /locus_tag="VF516_28165"
     CDS             complement(2038..>2685)
                     /locus_tag="VF516_28165"
                     /inference="COORDINATES: protein motif:HMM:NF024053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8111651.1"
                     /translation="RAACERELRATIQRLAHTPRWRALTVEERRRAARIAWYELQRRF
                     FRGFGKAAKDTERSATRALRNARGARQAAAQESLDAARVGAEVAEVEPVAGRLPRNHE
                     LAGGEFPREQLPPKYRAKGLRFKSTGYPDFEPYAMTLSNGKKTVRIELTGSMRADESL
                     ANNAAKLEETPEGYTWHHVEDEGTMMLVPTDLHHTVSHTGGRVKFKHRTGMRYGN"
BASE COUNT          414 a          928 c          894 g          449 t
ORIGIN      
        1 cgccgacccc acgagccggc cgctcgacga cgagttcgtc cgccacttct acgccggcac
       61 gacgtcgatc gaggccgccg acgacaaggg ctgggtggtc tcggtcacgc cgagcggtgg
      121 ctgggtcccc gccgtgatcg ccggcaagac cggcatcggc ctgagccagc ggatgcagag
      181 cttcgtgctc gaccccgccg agaacccgtt caacgtcgtc gagccgggca agcacccgcg
      241 cgtgacgctg accccgaccc tggcgctcaa ggacggcaag ccctacctgt cgttcgcggt
      301 ccagggcggc gactcgcagg accagaacct cttgcagttc ttcctcaacg tcgtcgagtt
      361 cgggatgaac gtccaggagg cgtgcgaggc tcccaacatc aacagctacc agatgcgcag
      421 ctcgttcggc gcgcacgacg cgcggccggg caagatgctg atcaacgagc agctgccggc
      481 gtgggtgcgc ggcgagctcg gcaagatggg ctacagcttc gaggtcaagc cccgcaccac
      541 ggggccgatc aacgcgatct tcttcgacca gccgcacggc acgttctggg gcggctcgag
      601 cgacgacggc gacgactacg gcatcgcgtg gtagcgcgcc ccgggcggag ccggccgcgg
      661 tgatcgggat ccggcgcgcc gaccagctcg cccgcggccg gtgaccaggc aacccgcgtc
      721 agtcctccag gtaggatcga accttcgacc gccagaaggc ttcgaggtcc gtggacgccg
      781 cctcccatgt cgaggtcgcg tattcctcct tatattccag ggacgggaac aggaagccga
      841 tgcttcgcac cacggccccg ctcgcatcga tcgcatagac atgatcgagc tcgccgtcga
      901 actcgtgcgc aaacaccaga taaccgggag gcagtgtcgg attgctccgg ttgaacgcca
      961 tgatgtcgtc atcgatcgtg gagctcgggc tcagtccgag tagcagcagg tgcggccggc
     1021 tgtcgatcgg acgttcgata tcgatgaccc cgaactcggt gatccaccac cggatcgggt
     1081 cagggacgtg tgcgggcagc gcctgcagca cgggctcgat gtcaccggca accagcggcc
     1141 tgagcgatcg ggtccgcggg tgacgcgcga gtacctgaat gtctcctggg gtgagggtca
     1201 tctgtgttcc gtgttgccta ctccgagccc ggcgtggacg gctcgcccgg agcaaccgat
     1261 tccaggatct tgaccgacca tcctgccgtg tccagatttc gaccggtcgg attcgtctcg
     1321 gcgaacgtgc cgccgaacat atctccggag gggctggctc gtggggtcac cgggaagctg
     1381 tattcataat tcgcgtacga gatcccgaaa tcaggtttgt gccacatccg ttcgccgccg
     1441 acggcgcact tgcatcgcgg gggcgtcatc gtcgctgcga gcgtagcgct tcatggctga
     1501 cccgcggctc gccgtcacgg agagccgctc gcgccgaggt cgtcgtcgag cgggcgcagg
     1561 ctggccatga actcggcgaa gctgccggcg atcttccaga cgtcgcgccg atcgaaccac
     1621 tcgacgcgcg ggttcgcgtc ggtcggacgc gggtcgccgg tgtcgaggta ccagacctcg
     1681 ccgcggtggg gcccgaccag tgacagaacg atccggccgc ccatgccgtc gtacccgatc
     1741 tcgaggagcc catcgggaag cttgacgtcg gggttgtagg gcttctgtgc cgtcgccagg
     1801 tcacgcgagt cgtcggagtc gtcgaggctg aacaggctgt tgagcacagt ctcgtcgcgc
     1861 cgtctgcccg cccgctgctg cacgatgaag acgcgatgcg atcgcgcggt tcgtcccccg
     1921 ttgacctcga gcaggaagcg ccggtagtcg tcggggatct cgaggccgag ctcgcgctca
     1981 aaacgtcgaa ctgcgtcgac ggtgaccgcg tacccgcgcc gggccatcgg tggccagcta
     2041 gttgccatag cgcatccccg ttcgatgctt gaacttcaca cggccgccgg tgtggctgac
     2101 cgtgtgatgg agatccgtcg gcacgagcat cattgtgccc tcgtcttcga cgtggtgcca
     2161 ggtgtaacct tcaggcgttt cctcgagctt cgccgcgttg ttggccagcg actcgtcggc
     2221 gcgcatgctc ccggtcagct cgatccgcac cgtcttcttg ccattcgaga gcgtcatcgc
     2281 gtatggctcg aagtccgggt agcccgtcga cttgaaccgc aaacccttcg cccggtactt
     2341 cggcggcagc tgctcgcgcg ggaactcgcc gccagcgagc tcgtggttgc gcggcagccg
     2401 gccggcaacc ggctcgacct cggccacctc ggcgccgacg cgcgcggcgt cgaggctctc
     2461 ctgcgccgca gcctggcgcg cgccgcgcgc gttgcgcagc gctctcgtcg ccgaccgctc
     2521 ggtgtccttg gccgccttcc cgaacccgcg gaagaaccgc cgctgcagct cgtaccacgc
     2581 gatgcgcgcc gcgcgccgcc gctcctcgac ggtgagcgct cgccacctcg gcgtgtgcgc
     2641 cagcctctgg atcgtcgcgc gcagctcgcg ctcgcaggcc gcacg
//
LOCUS       DASVOR010001488         2179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_103569,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001488 DASVOR010000000
VERSION     DASVOR010001488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103569"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2179
                     /gene="uvrA"
                     /locus_tag="VF516_28170"
     CDS             <1..>2179
                     /gene="uvrA"
                     /locus_tag="VF516_28170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX8111652.1"
                     /translation="RSLRDGAVAVWGEAIAKDSGWTTNIVKALAKAFKIDLDRPWNKL
                     SDKQREVLLYGTGDKRVTVAWEGRHGAGEWAMRFEGILPQLERRHRESSSDRTKQHYE
                     QFFRAIPCAVCQGTRLRPESRAVFVADRPIVEVTGMTVREAYDFIAGMKLTGSRAAIA
                     GEVLKEIRARLTFLLDVGLDYLTLHRAAGTLSGGEAQRIRLASQLGSELSGVLYVLDE
                     PSIGLHQRDNERLIRTLHRLRDLGNTVLVVEHDEATIEAADWVVDFGPGAGRHGGRVV
                     AEGTPGDIKANADSITGRFLAGAERIDVPAERRAPTSWVKLTGAREHNLRNVTVEIPL
                     GVMVAVTGVSGAGKSSLINATLYPALNRMLHRSTERVGPYDELTGLGKIDKCIVIDQQ
                     PIGRTPRSNPATYTKAFDLIRELYAQVPQARTYGYQAGRFSFNVSAKQGGGRCEACEG
                     AGVREVEMHFLPNVFVTCEVCKGKRYNDATLRVTYKDKNIAEILDTPIDEAAVLFQHH
                     KQLSRIMTTLVDVGLGYLALGQPATTLSGGEAQRVKLARELARVQTGRTLYLLDEPTT
                     GLHFGDVKKLLEVLQRLVDGGNTVLVIEHNLDVIKTADWIIDMGPEGGAAGGQVIATG
                     TPEQVARIAASYTGQFIKPLLERSRRSADGNGGGRPRRDRPQDQRARRARSRLPHARP
                     PVDDPLGRRSAAREARRRARQAQARQTQPVHPRRADDGPALRRH"
BASE COUNT          351 a          801 c          747 g          280 t
ORIGIN      
        1 ccggtcgctg cgcgacggcg cggtcgcggt gtggggcgag gcgatcgcca aggactcggg
       61 ctggaccacc aacatcgtca aggcgctggc caaggcgttc aagatcgacc tcgaccggcc
      121 gtggaacaag ctgtccgaca agcagcgcga ggtgctgctg tacggcaccg gcgacaagcg
      181 cgtcacggtc gcgtgggagg gccggcacgg cgccggtgag tgggcgatgc ggttcgaggg
      241 catcctgccc cagctcgagc gccgccaccg cgagtcgtcg agcgatcgca ccaagcagca
      301 ctacgagcag ttcttccgcg cgatcccgtg cgcggtgtgc cagggcacgc ggctgcgccc
      361 cgagtcgcgc gcggtgttcg tcgccgaccg gccgatcgtc gaggtcaccg ggatgacggt
      421 gcgcgaggcc tacgacttca tcgccgggat gaagctcacc ggctcgcgcg ccgcgatcgc
      481 cggcgaggtg ctcaaggaga tccgcgcccg gctgaccttc ctgctcgatg tcgggctcga
      541 ctacctgacc ctgcaccggg cggccggcac gctgtcgggc ggcgaggccc agcggatccg
      601 gctggcgtcg cagctcggca gcgagctgtc cggcgtgctg tacgtgctcg acgagccgtc
      661 gatcggcctg caccagcgcg acaacgagcg gctgatccgc acgctgcacc ggctgcgcga
      721 cctcggcaac accgtgctcg tggtcgagca cgacgaggcc acgatcgagg ccgccgactg
      781 ggtggtcgac ttcggcccgg gcgcgggccg ccacggcggc cgggtcgtcg ccgagggcac
      841 ccccggtgac atcaaggcca acgcggactc gatcaccggg cggttcctcg ccggcgccga
      901 gcggatcgac gtcccggccg agcgccgcgc gccgaccagc tgggtcaagc tgaccggcgc
      961 gcgcgagcac aacctgcgca acgtcaccgt cgagatcccg ctcggcgtca tggtcgcggt
     1021 caccggcgtg tcgggcgccg gcaagtcgtc gctgatcaac gcgacgctct accccgcgct
     1081 caaccgcatg ctgcaccgct cgaccgagcg ggtcgggccg tacgacgagc tgaccggcct
     1141 gggcaagatc gacaagtgca tcgtgatcga ccagcagccg atcggccgca cgccgcgctc
     1201 caacccggcg acctacacca aggcgttcga cctgatccgc gagctctacg cccaggtgcc
     1261 gcaggcccgg acctacggct accaggccgg ccggttctcg ttcaacgtct cggccaagca
     1321 gggcggcggg cgctgcgagg cctgcgaggg cgcgggcgtg cgcgaggtcg agatgcactt
     1381 cctgcccaac gtgttcgtca cctgcgaggt ctgcaagggc aagcgctaca acgacgccac
     1441 gctgcgcgtg acctacaagg acaagaacat cgccgagatc ctcgacacgc cgatcgacga
     1501 ggccgcggtg ctgttccagc accacaagca gctgtcgcgg atcatgacca cgctggtcga
     1561 cgtcggcctg ggctacctgg cgctcggcca gcccgcgacc acgctgtcgg gcggcgaggc
     1621 ccagcgcgtc aagctcgccc gcgagctcgc ccgggtccag accggccgca cgctgtacct
     1681 gctcgacgag ccgaccaccg gcctgcactt cggcgacgtc aagaagctgc tcgaggtcct
     1741 ccagcgcctg gtcgacggcg gcaacaccgt gctcgtcatc gagcacaacc tcgacgtcat
     1801 caagaccgcc gactggatta tcgacatggg ccccgagggc ggcgccgccg gcggccaggt
     1861 gatcgccacc ggcacccccg aacaggtcgc gcggatcgcg gccagctaca ccggccagtt
     1921 catcaagccg ctgctcgagc ggtcccggcg cagcgccgac ggcaacggcg gcggccgccc
     1981 gcgccgcgat cgcccgcaag atcagcgtgc tcgacgagct cggtctcggt tacctcacgc
     2041 tcggccaccc gtcgacgatc ctctcgggcg gcgaagcgca gcgcgtgaag ctcgccggcg
     2101 agctcggcaa gctcaagcgc ggcaaacaca acctgtacat cctcgacgag ccgacgacgg
     2161 gcctgcactt cgccgacat
//
LOCUS       DASVOR010001489         4635 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_511961,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001489 DASVOR010000000
VERSION     DASVOR010001489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4635
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_511961"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3520)
                     /locus_tag="VF516_28175"
     CDS             complement(<1..3520)
                     /locus_tag="VF516_28175"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HEX8111653.1"
                     /translation="MQADTHAAAPFVVALGASADGLDALERFFAHVPPRTGMAFVVIQ
                     HLSAEFDSHMADILSRRTKLPVRVATHGARLEPDTVYLNPPKHVMTLDHGRLQLADRA
                     PGPAVPNSIDRFLQSLAIDAAGRCAAIVLSGAGSDGSRGIIDVHGAGGLVIVQTPATA
                     GFDGMPLAAIDTGHADLMLAPEDMPEALRAYLADGGVEPQPETATHSGLARLISLLSD
                     AYGIDFFEYKLPTIARRTERRMKAAGVTDLDAYLARLASDRAELDALYHDLLIGVTQF
                     FRDREAFAELAKLLPEQLRALGPEEEFRVWVVACASGEEAYSIAILVREALDALGLAN
                     PVKIFATDIHREALRVAGAGIYGEASLALISPERRQRWFTSKGGRKYQIAPAIRSMLV
                     FAPHNVLQDVPFNRLDAISCRNLLMYLRPAAQRRVLDRFCYALKPRGIAFLGSSEPLS
                     ELGSSFQILDAHWKIVRKQSSGPRAAHRPRLAATAPTPPRLGGAAAVTRLEPNLFGTY
                     DALLDALVPPSILVDARGHLVQTFAGASRFLRVPDGPATWSAIDLLGDDLRIAVTGGL
                     ERVFADHAPVHYRRLRAELGGQVELVNVTVREIVNRRTGDLHALITFEPDSTAARPAD
                     LSPAVPSDQLARDQVAALERALRTVKENLQATTEALETSNEELQATNEELVASNEELK
                     STNEELHSVNHELEDVNRQLQRKIDELTQLATDMDLLVGSTEVHTLFLDKELRIRRFT
                     PLIAEVFHLVPSDLGRRIDGFNHALRAPAIYAEAQQVLATGRPFEHQVRGAGDTWYLL
                     RILPCRRGVVTEGAVLTLVDITNLKRFELEAQNRRDQLSSILANSPDPVWIRDRDGRY
                     VVADDSFRKLTGRDPTGLRPEQVFSADVAAMLTRDDTRVLDHGATVQAEETIPTPSGP
                     RTFLSVKFPMTDAAGNRWGIGGIQTDVSALKRAEAEAREAADRRDHFLASLSHELRNP
                     LAAILNAARVLGHGPLPAEDTATWHRIVLERAQHMVRLVDDLLDVARLTQNKLVLKRT
                     QLDLGATTRGVVEEVDPEFRERGVALRVRADDPLIVFGDATRLHQLQVNLLTNAARHA
                     PPGSEVSYTVRRADDSAEICVTDTGSGIAPEILGKIFELFVQGDRPGSRGGNGGLGVG
                     LALVRRIAELHGGTAVASSPGVGRGAE"
     gene            3739..4122
                     /locus_tag="VF516_28180"
     CDS             3739..4122
                     /locus_tag="VF516_28180"
                     /inference="COORDINATES: protein motif:HMM:NF018228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8111654.1"
                     /translation="MDDDANQAGALAALLQLDGVAAISEHVAAAALARAMIEPPDAIV
                     LNVKMPGVSGTELLAALRARHPDLPVLLLTGYDMHDPRLAAALASGSVGYLAKPVELP
                     RLVEMLGRLFEQRGIPKPVRPEGAS"
     gene            4220..4633
                     /locus_tag="VF516_28185"
     CDS             4220..4633
                     /locus_tag="VF516_28185"
                     /inference="COORDINATES: protein motif:HMM:NF022591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HEX8111655.1"
                     /translation="MTKSTVLLVIALAAGTAYAEDPKPPPAAADTKPKDAGSASAEVK
                     AGTGVEKHAIVGEAASFPAGTTVWVWSQITGGEGNIKHVWKRDGKEVWSATLPVGSKR
                     WATSSRRTIPSAGSWQVDVTTEAGQQIGTVSFTIQ"
BASE COUNT          662 a         1588 c         1703 g          682 t
ORIGIN      
        1 actcggcgcc gcgcccgacg ccggggctcg acgcgacggc cgtgccgccg tggagctcgg
       61 cgatccgccg caccagcgcc agcccgaccc ccaggccgcc attgccgccg cgcgatccag
      121 ggcggtcgcc ctgcacgaac agctcgaaga tcttgccgag gatctccggg gcgatccccg
      181 aaccggtgtc cgtcacgcag atctccgccg agtcatcggc gcggcgcacg gtgtaggaga
      241 cctcgctccc gggcggcgcg tggcgcgcgg cgttggtcaa gaggttgacc tgcagctggt
      301 gcagccgggt cgcgtcgccg aacacgatga gcgggtcgtc ggcgcggacg cgcaacgcga
      361 cgccgcgctc gcggaactcg gggtcgacct cctcgaccac gcctcgggtc gtcgcgccca
      421 gatcgagctg ggtccgcttg agcacgagct tgttctgggt gagccgtgcc acgtcgagca
      481 ggtcgtcgac cagccgcacc atgtgctggg cgcgctcgag gacgatccgg tgccacgtcg
      541 cggtatcctc ggcgggcagc ggaccgtgcc cgagcacgcg ggcggcgttg aggatcgccg
      601 ccagcgggtt gcgcagctcg tgcgacagcg acgcaaggaa gtgatcgcgg cgatccgcgg
      661 cttcgcgcgc ctctgcctcg gcgcgcttga gcgcgctgac gtcggtctgg atgccgccga
      721 tgccccagcg gttccccgcc gcgtcggtca tcggaaactt caccgacagg aacgtccgcg
      781 ggccgctcgg cgtcgggatg gtctcctcgg cctgcaccgt cgcgccgtgg tcgagaacgc
      841 gggtgtcgtc gcgcgtcagc atcgccgcga cgtccgccga gaacacctgc tcgggccgga
      901 gcccggtcgg gtcgcggccg gtgagcttgc ggaagctgtc gtcggcgacc acgtagcgcc
      961 cgtcgcggtc gcggatccac accggatccg gtgagttggc caggatgctc gacagctggt
     1021 cccgccggtt ctgcgcctcg agctcgaatc gcttgaggtt ggtgatgtcg acgagcgtca
     1081 gcaccgcgcc ctcggtcacc acgcctcgtc gacagggcag gatgcgcagc aggtaccagg
     1141 tatcgccggc gccgcggacc tggtgctcga acggcctgcc ggtggcgagc acctgctggg
     1201 cctcggcgta gatcgccgga gcgcgcagcg cgtggttgaa cccgtcgatc cggcggccga
     1261 ggtcgctcgg cacgagatgg aacacctcgg cgatcagggg cgtgaaccgc cggatccgca
     1321 gctccttgtc gaggaacagc gtgtggacct cggtgctgcc gaccaggaga tccatgtcgg
     1381 tcgcgagctg ggtgagctcg tcgatcttgc gctggagctg gcggttcacg tcctccaact
     1441 cgtggttgac cgagtgcagc tcctcgttgg tcgacttgag ctcctcgttg gaggcgacca
     1501 gctcctcgtt ggtcgcctgc agctcctcgt tgctggtctc gagcgcctcg gtcgtggcct
     1561 gcaggttctc cttcacggtc cgcagcgcgc gctcgagcgc cgcgacctgg tcgcgcgcca
     1621 gctggtcgct cgggacggcc ggggacaggt cggccgggcg cgccgctgtg ctgtcgggtt
     1681 cgaacgtgat cagcgcgtgc agatcgccgg tgcggcggtt gacgatctcg cgcacggtca
     1741 cgttgacgag ctcgacctgg ccgccgagct cggcgcgcag ccggcgatag tgcacggggg
     1801 cgtgatcggc aaacacgcgc tccagcccac cggtgaccgc gatgcgcagg tcgtcgccga
     1861 gcaggtcgat cgcgctccag gtcgcggggc catcgggcac gcgcaggaac ctgctcgccc
     1921 cggcgaacgt ctgcaccagg tggccgcggg cgtcgaccag gatgctcggc ggcacgagcg
     1981 cgtcgagcaa cgcatcgtag gtcccgaaca ggttgggctc gagccgggtc accgccgcgg
     2041 ccccccccag gcggggcggg gtcggcgcgg tggcagccag gcgtggccgg tgcgccgcac
     2101 gggggcccga cgattgcttg cgaacgatct tccagtgggc gtcgaggatc tggaacgagc
     2161 tcccgagctc ggacagcggc tcgctcgatc cgaggaacgc gatgccgcgc ggcttgaggg
     2221 cgtagcagaa ccggtccagc acgcgccgct gcgcggcggg tcggaggtac atcagcaggt
     2281 ttcggcagct gatggcgtcg agccggttga acggcacgtc ctgcagcacg ttgtggggcg
     2341 cgaacacgag catcgagcgg atcgccggcg cgatctggta cttccggccg cccttgctgg
     2401 tgaaccagcg ctgcctgcgc tcgggggaga tcagcgccag gctcgcctcg ccgtagatcc
     2461 cggctccggc gacccgcagc gcctcgcgat ggatgtcggt cgcgaagatc ttcaccgggt
     2521 tcgcgagccc gagcgcgtcg agcgcctcgc ggaccaggat cgcgatcgag tacgcctcct
     2581 cgcccgacgc gcaggccacc acccagacgc gaaactcttc ctcgggcccc agcgcacgca
     2641 gctgttcggg caggagcttg gcgagctcgg caaacgcctc gcggtcccgg aagaactggg
     2701 tgaccccgat cagcagatcg tgatacagcg catcgagctc ggcccgatcg gacgcgagcc
     2761 gcgcgaggta ggcatcgaga tcggtgacgc cggccgcctt catgcggcgc tcggtccggc
     2821 gcgcgatggt cgggagcttg tactcgaaga agtcgatgcc gtaggcgtcg gacagcaggc
     2881 tgatcagtcg cgccagaccg ctgtgggtcg cggtctcggg ctgcggctcc acgccgccgt
     2941 ccgcgaggta cgcccggagc gcctccggca tgtcctcggg ggcgagcatg agatcggcgt
     3001 gtccggtgtc gatcgcggcc agcggcatcc cgtcgaaccc tgcggtggcc ggggtctgca
     3061 cgatgaccag gccgcccgcg ccgtgcacgt cgatgatgcc gcgcgagccg tcgcttccgg
     3121 cgcccgacag cacgatcgcc gcgcagcgtc ccgcggcgtc gatcgccagc gactggagga
     3181 accggtcgat cgagttgggg accgccgggc ccggtgcacg gtcggcgagc tggaggcggc
     3241 catggtcgag ggtcatcacg tgcttcggcg ggttcaggta caccgtgtcc ggctcgaggc
     3301 gagcgccgtg cgtcgcgacg cgcaccggca gcttcgtgcg ccgcgacagg atgtccgcca
     3361 tgtggctgtc gaactcggcc gacaggtgct ggatcacgac gaacgccatc ccggtgcgcg
     3421 gcgggacgtg cgcgaagaac cgctcgagtg catcgagccc gtccgccgac gcgccgagcg
     3481 ccaccacgaa cggagccgcc gcgtgcgtgt ccgcttgcat ctcgcgcatg atggccggaa
     3541 tgcaccggcc acgcctgcca aggtctgccc gaaagccggc aaaccgatgc aatgttcgcg
     3601 ctgccgtcga actgggcaca atcattgcgt gagccggctc cccacgggcc gggcgcaggg
     3661 tcgagcatcg gctattcttg gccctgcatg tcagtcgcaa ggcctgcgca ttccctcgcg
     3721 gcgcgtcgga tcctgatcgt ggatgacgat gcgaaccagg ccggcgcgct ggccgcgctg
     3781 ctccagctcg acggggtcgc cgcgatcagc gagcacgtgg ccgccgcggc gctcgcgcgt
     3841 gcgatgatcg agccgcccga cgcgatcgtg ctcaacgtca agatgcccgg tgtctcggga
     3901 acagagctcc tcgccgcctt gcgcgcccgc catccggacc ttcccgtgct gttgctgacg
     3961 gggtacgaca tgcacgatcc caggctcgcg gcggcgctgg cgtccggcag cgtggggtac
     4021 ctggccaagc cggtggagct gcccaggctg gtcgagatgc tcggccggct gttcgagcag
     4081 cgcgggatcc cgaagccagt tcgccctgag ggggcgagct gatcgccgcg cacgggtgta
     4141 tcgtgagcga tgccggacgc cacgtcatcg ggggatgact tcggcgcggt ccggcgttgt
     4201 tcaggacgga gggaatgcca tgacgaaatc gactgtcttg ctcgtgatcg cgctcgcggc
     4261 ggggaccgcc tacgccgaag atccgaagcc gccgcccgcg gcagcggaca ccaagcccaa
     4321 ggacgccggc agcgcgtcgg ccgaggtcaa ggccggcacc ggggtggaga agcacgcgat
     4381 cgtcggggag gcggccagct tccccgccgg caccacggtg tgggtgtggt cgcagatcac
     4441 gggcggcgag ggcaacatca agcacgtgtg gaagcgcgac ggcaaggagg tgtggagcgc
     4501 cacgctgccg gtcggctcca agcgctgggc gacgtcgtcg cggcgcacga tcccgtcggc
     4561 gggcagctgg caggtcgacg tgaccaccga ggccggccag cagatcggca ccgtgtcgtt
     4621 cacgatccag tgacg
//
LOCUS       DASVOR010001490         2718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_423663,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001490 DASVOR010000000
VERSION     DASVOR010001490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423663"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="VF516_28190"
     CDS             <1..2134
                     /locus_tag="VF516_28190"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEX8111656.1"
                     /translation="SGRLDDLWLGSVRVWMLICFGFLSLGLILGGRWAYEELGWGGYW
                     AWDPVENAGFVPWFTATAFLHSAIIQEQRGMMKRWNMLLVIVTFFFTIFGTFMTRAGI
                     VQSVHAFGEDNVLALQFVAFLVLIVVVSIGLLVYRWNKLASRGEFESFISREFAFLVN
                     NWILLACAFVVLFLTMLPTMSEALDGTRMAVGPEFFNKSMTPLGLTLLFLAGAAPLLA
                     WHRTTRERLYAQFLWPCVAIGATIIGMLVVFPEAKHLSAIFSDVIKLPVPLINFGLVA
                     FTIASIVQEYWRGVGVRKRQSGSDALTSLLGLVLSKRRKYGGYIVHLGIAVLFWGFAG
                     KAYERMIDRTIEKPAMSLADKDKAKARFVFGEYEFEYERLIEGGDDHKNTVTAEVTIF
                     HAGKKIDMAYPAKWDYHKGEGQATTEVAIIVRAGVIDVVRGDTVVARELGEVFGNDVY
                     LVLTGYDTTSGLANLRVYINPHILWVWIGFLILAFGTLVCLIPQIVVDRLSRPRASRL
                     GRAADLSVVIALTCAAVLGIASQGHAAPPPAEHAQAGMGMGADGIGYASMNRPTNATE
                     ERAMKELLCICGCAREDIFGCKCGTAAQLRRQVMDFLAKVDASGKPVFDLTTPAGRDA
                     AYGAVLDDFVKTYGGEQVLATPRTQLSWLLPSLAVLGGLGLLFVVGRRWLGHGAATAV
                     PAAPGGAAAVAEDESYADKLDDELADTD"
     gene            2169..>2718
                     /locus_tag="VF516_28195"
     CDS             2169..>2718
                     /locus_tag="VF516_28195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111657.1"
                     /translation="MTDSPDTMRWVWRGVAIAILVIGWVLFAWLYHFRITAPLVFICL
                     GYLAALVTIYNLWRTGAAAVDSSEDDDDSTWAKPAGAFGELAREKRTLLKAIKEAEFD
                     RDMGKLSQRDADEMIRGYRARAIEVIKQIDQLGLGAAGTVREQILREVRARVQLDAKA
                     ARKPGPEARPGKAKAETRPEAKT"
BASE COUNT          395 a          920 c          960 g          443 t
ORIGIN      
        1 gtcgggccgg ctcgacgacc tgtggctggg ctcggtccgg gtctggatgc tgatctgctt
       61 cggcttcctg tcgctcggcc tgatcctcgg cgggcggtgg gcctacgagg agctgggctg
      121 gggcggctac tgggcgtggg acccggtcga gaacgccggc ttcgtcccgt ggttcaccgc
      181 gaccgcgttc ctgcactcgg cgatcatcca ggagcagcgc ggcatgatga agcgctggaa
      241 catgctgctc gtgatcgtga cgttcttctt cacgatcttc ggcacgttca tgacccgcgc
      301 cgggatcgtg cagtcggtcc atgcgttcgg tgaggacaac gtgctcgcgc tgcagttcgt
      361 ggcgttcctg gtcctgatcg tcgtggtgtc gatcggcctg ctggtctacc gctggaacaa
      421 gctcgcgtcg cgcggcgagt tcgagtcgtt catctcgcgc gagttcgcgt tcctggtcaa
      481 caactggatc ctgctggcct gcgcgttcgt cgtgctgttc ctgaccatgc tgccgacgat
      541 gtccgaggcg ctcgacggca cgcggatggc ggtcggcccc gagttcttca acaagtcgat
      601 gacgccgctc ggcctgacgc tcctgttcct ggccggggcg gcgccgctcc tggcctggca
      661 caggaccacg cgcgagcggc tgtacgcgca gttcctgtgg ccgtgcgtcg cgatcggggc
      721 gacgatcatc gggatgctgg tggtgttccc cgaggccaag cacctgtcgg cgatcttctc
      781 cgacgtgatc aagctgccgg tcccgctgat caacttcggg ctggtcgcgt tcaccatcgc
      841 gtcgatcgtg caggagtact ggcgcggcgt cggcgttcgc aagcggcagt ccggctcgga
      901 cgcgctgacc tcgctgctcg gcctggtgct gtccaagcgc cgcaagtacg gcggctacat
      961 cgtccacctc gggatcgccg tgctgttctg gggattcgcc ggcaaggcgt acgagcggat
     1021 gatcgatcgc acgatcgaga agcccgccat gtcgctcgcc gacaaggaca aggcgaaggc
     1081 gcggttcgtg ttcggcgagt acgagttcga gtacgagcgc ctgatcgagg gcggcgacga
     1141 ccacaagaac accgtgaccg ccgaggtcac gatcttccac gccggcaaga agatcgacat
     1201 ggcctacccg gcgaagtggg actaccacaa gggcgagggc caggcgacga ccgaggtcgc
     1261 gatcatcgtg cgcgccggcg tgatcgacgt cgtgcgcggc gacaccgtcg tcgcgcgcga
     1321 gctcggcgag gtgttcggca acgacgtcta cctcgtgctg accggctacg acaccacctc
     1381 ggggctcgcc aacctgcgcg tgtacatcaa cccgcacatc ctgtgggtgt ggatcgggtt
     1441 cctgatcctc gcgttcggca cgctggtctg cctgatcccg cagatcgtgg tcgaccggct
     1501 gtcgcggcca cgggcgagcc ggctcggtcg cgccgccgat ctgtcggtgg tgatcgcgct
     1561 gacctgcgcg gcggtcctgg gcatcgcgag ccagggccac gcggcgccgc cgcccgccga
     1621 gcacgcccag gccggcatgg ggatgggcgc cgacggcatc ggctacgcgt cgatgaaccg
     1681 gccgaccaac gccaccgagg agcgcgcgat gaaggagctg ctgtgcatct gcggctgcgc
     1741 gcgcgaggac atcttcggct gcaagtgcgg caccgccgcg cagctgcgcc gccaggtgat
     1801 ggacttcctc gccaaggtcg acgccagcgg caagccggtg ttcgacctga ccaccccggc
     1861 cggccgcgac gcggcctacg gagcggtgct cgacgacttc gtcaagacct acggcggcga
     1921 gcaggtcctg gcgaccccgc gcacccagct gtcgtggctc ttgccgtcgc tcgccgtgct
     1981 gggcggcctg ggcctgctct ttgtcgtcgg ccggcgctgg ctcggccacg gcgcggcgac
     2041 cgcggtcccg gccgctcctg ggggggccgc cgcggtcgcc gaggacgaat cctacgccga
     2101 caagctcgat gacgagctcg cggacaccga ctgagcgtgc cgggcgcgca accccaagcg
     2161 tgaatgacat gacggattcc cccgacacga tgcgatgggt ctggcgcggc gtggccatcg
     2221 ccatcctggt gatcggctgg gtgctcttcg cctggctgta ccacttccgc atcaccgcac
     2281 cgctggtgtt catctgcctc ggctacctcg ccgcgctcgt gacgatctac aacctgtggc
     2341 ggaccggcgc ggccgcggtc gacagctccg aggacgacga cgactcgacc tgggccaagc
     2401 cggccggggc gttcggcgag ctggcgcgcg agaagcgcac gctgctcaag gcgatcaagg
     2461 aagccgagtt cgaccgcgac atgggcaagc tgtcccagcg cgacgccgac gagatgatcc
     2521 gcggctaccg ggcgcgcgcg atcgaggtga tcaagcagat cgaccagctc ggcctcggcg
     2581 ccgccggaac cgtgcgcgag cagatcctgc gcgaggtgcg cgcccgggtc cagctcgacg
     2641 ccaaggcggc caggaagccg ggacccgagg cgcggccggg caaggccaag gcggagacca
     2701 ggcccgaggc caagaccg
//
LOCUS       DASVOR010001491         1670 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_73484,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001491 DASVOR010000000
VERSION     DASVOR010001491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1670
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73484"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..424
                     /locus_tag="VF516_28200"
     CDS             <1..424
                     /locus_tag="VF516_28200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111658.1"
                     /translation="PPELPAIDAGAPPAPGHVLVHNDVWCNIWIDGANHGNRRNEPIE
                     VSAGHHVVRCVNPAGEWTQDAEVAPGTTRILSGTLLRDVQVTLDVDATIDGKPYPSGA
                     VVRLKPGNIEVTAGGKKQFITFRANCRLKDTPDLGCYL"
     gene            531..1643
                     /locus_tag="VF516_28205"
     CDS             531..1643
                     /locus_tag="VF516_28205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111659.1"
                     /translation="MRAVAAVWVLLTGCFYIDPILSRPRSLIIRPDVITRGGVVTLAA
                     QLVDAESQAGSFDWTVSTCSGFDQGVASGCDSAPFYPPPGPRPDDSHSTVSFTVPPMT
                     RSGARTQAIQVTLDARNDRGALAQSDEQYAVGDAPPAFQLDRQSHSLAVGAPIDVIAT
                     YSDVDSALDGVVLAWTATAPGSGGSFPLEEIAIAQDPGDPGHRKAGKRLTPTVTGNWD
                     VQVTARDPEGGSTTHDLPFTVVADQPPCLQQWLPTVPPAGATLPISTPTVFQVPLVDD
                     DLDPYPPIEAAPQLTFAWSILRPRTTQREVQTGATGNAIVLDPAVFTPGDVVELRVEI
                     YDRQHTALPCDDAAATCSITSSDRCLQRQTWRVEVR"
BASE COUNT          250 a          623 c          571 g          226 t
ORIGIN      
        1 accgcccgag ctcccggcga tcgacgccgg cgccccgccc gcaccgggcc acgtcctcgt
       61 gcacaacgac gtgtggtgca acatctggat cgacggcgcg aaccacggca accggcgcaa
      121 cgagccgatc gaggtcagcg ccggccacca cgtcgtgcgc tgcgtcaatc cggcgggcga
      181 gtggacgcag gacgccgagg tcgcgccggg gacgacccgg atcctgagcg gcacgctgct
      241 gcgcgacgtg caggtgacgc tcgacgtcga cgcgacgatc gacggcaagc cgtacccgag
      301 cggcgccgtg gtccggctca agccgggcaa catcgaggtc accgccggcg gaaagaagca
      361 gttcatcacg ttccgcgcca actgccggct caaggacacc ccggacctcg gctgctacct
      421 gtgactggaa tcgcgacagc ggtgtcgcga tccgcgagcc cggggccgct ccggcgcgtt
      481 gtgatctgcc cacttgcgca tggcaaggtg attgcacgag ggtggtcggc gtgagggccg
      541 tcgcggctgt ctgggtcctt ctgaccgggt gcttctacat cgacccgatc ctctcgcggc
      601 cgcgctccct catcatccgg ccggacgtga tcacccgcgg cggtgtcgtc accctggccg
      661 cccagctggt cgacgcggag agccaggcag gctccttcga ctggacggtg tcgacctgtt
      721 ccggcttcga tcagggcgtg gccagcgggt gcgacagcgc gccgttctac ccgccgcccg
      781 gtccccggcc cgacgactcc cacagcaccg tgagcttcac ggtcccgccg atgacgaggt
      841 ccggggccag gacccaggcg atccaggtca cgctggacgc gcgcaacgat cgcggcgcac
      901 tcgcccagtc ggacgagcag tacgcggtcg gcgatgcgcc gccggcgttc cagctcgacc
      961 ggcaatcgca cagcctcgcg gtcggtgcgc cgatcgacgt gatcgccacg tacagcgatg
     1021 tcgacagcgc gctcgacggc gtcgtgcttg cctggaccgc gaccgcgccg ggctcggggg
     1081 gatcgttccc gctcgaagag atcgcgatcg cgcaggatcc cggcgacccg ggccaccgca
     1141 aggcgggcaa gcggctgacc ccgaccgtga caggcaactg ggacgtgcag gtcaccgcgc
     1201 gcgatccgga gggaggctcg accacccatg acctgccgtt cacggtcgtc gccgaccagc
     1261 cgccatgcct ccagcagtgg ctgccgaccg tcccgccggc cggcgcgacc ttgccgatct
     1321 cgacgcccac ggtgttccag gtccccctgg tcgacgacga cctcgacccc tacccgccga
     1381 tcgaagcggc gccgcagctg acgttcgcgt ggtcgatcct gcggccgcgc acgacgcagc
     1441 gcgaggtgca gaccggcgcc accggcaacg ccatcgtgct cgatcccgcc gtgttcacgc
     1501 cgggcgacgt cgtcgagctg cgggtcgaga tctacgatcg ccagcacacc gcgctgccgt
     1561 gcgacgacgc cgccgcgacc tgctcgatca cgtcgtccga ccgctgcctg cagcgccaga
     1621 cctggcgcgt ggaggtccga tgacgggcgc tcgatgggtc gcgcagggct
//
LOCUS       DASVOR010001492         3621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_16056,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001492 DASVOR010000000
VERSION     DASVOR010001492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3621
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16056"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VF516_28210"
     CDS             complement(<1..846)
                     /locus_tag="VF516_28210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111660.1"
                     /translation="MLRHVAGFEWRYQLRSPVFWVGCALFFLITFGAATIDQIQIGGR
                     GNVFVNSPYTILQTLSVLSLFSIFVIVAMVASVVIRDDETGFAPIIRVTSVGKFSYLV
                     GRFAGASAAALGVLAMVPLAIAIGAQMPWLDPEKVGPFRIGDYVYALFAFGLPTLLII
                     AAGYFALATATRSMMWTYVGAAATLIAYLAMRFALSDPQFDTVSALIDPFGRSTLIIV
                     TKYWTATERNTLLPPMSGLLLANRVLWSAVAIALFALAYRRFRFEQPGARAARRTAAE
                     DTAPPD"
     gene            complement(855..1757)
                     /locus_tag="VF516_28215"
     CDS             complement(855..1757)
                     /locus_tag="VF516_28215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111661.1"
                     /translation="MLTISGLSHSYDGRTRALDDVTLTIPRGMFGLLGPNGAGKSTLM
                     RCVATLQVPTAGSIVFDGIDVLAEPARLRAVLGYLPQDFGVYPRVSAYDMLDHLAVLK
                     GVIARGERREVVETLLHQVNLWHVRRRPIAGFSGGMRQRFGIAQALIGRPRLIIVDEP
                     TAGLDPEERNRFSNLLAEIGENMVVILSTHIVDDVTDLCSRVAVMVDGRIVANDTPAA
                     LIDVFRGKVWTRTIDKTELAACHARFGVISTRMRAGRTQVRVLADAAPEPAFEPVPSN
                     LEDVYFVTLYRHRRDAVAGAPGVR"
     gene            complement(2063..3196)
                     /locus_tag="VF516_28220"
     CDS             complement(2063..3196)
                     /locus_tag="VF516_28220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEX8111662.1"
                     /translation="MAPRTTTRTSITGYGVWHPPTVLSNEELCAAFNEFVRRDNAQNA
                     EAIAAGTRAPLRESTPEWIVKASGIQQRYVEDKTGLVDPERMCPNIPDRPEDQLSYQA
                     EYALNAGRRALEVAGRAGEDVDLVVLGCSNLQRMYPALAIEVQEALGAHGYGFDILLG
                     CSAATGATQLASQAVQLGQARCALVVIPELTTGHMNWRERDSHFIFGDASVSLVVEPT
                     DRARPGSWEILSTRMMSKWSSNIRNNRGYLDRCDPSTEHNIDKLFHQNGRRVFKDVVP
                     LAAKFIADHIAAHDLDPSQIGRYWLHQANQSMNELIAKRILGRDATREEAPIILDEYG
                     NTASAGSLIAFSHHNADLPAGSHGVMASFGAGYSLGSLLLQRQ"
     gene            complement(3358..>3621)
                     /locus_tag="VF516_28225"
     CDS             complement(3358..>3621)
                     /locus_tag="VF516_28225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111663.1"
                     /translation="PAVPTTAAIPAPPPSHGPVAAPDEAAGPAQRMSSKALVHYRKAE
                     LALRRGDLTGAVLHLKMAIASDPQSAFLRSALAEVEAEVMKKP"
BASE COUNT          530 a         1239 c         1314 g          538 t
ORIGIN      
        1 gtccggcgga gcggtgtcct cggcggcggt ccggcgcgcg gcccgcgccc cgggctgctc
       61 gaaccggaac cgtcgatatg caagcgcgaa cagcgcgatc gccaccgccg accacaggac
      121 gcggttggcg agcaggaggc ccgacatcgg cggcagcagc gtgttgcgct cggtggcggt
      181 ccagtacttg gtgacgatga tcagcgtcga acgcccgaac gggtcgatca gcgccgacac
      241 ggtgtcgaac tgcggatcgc tcagcgcgaa gcgcatcgcg aggtaggcga tcagcgtggc
      301 ggccgcgccg acgtaggtcc acatcatcga gcgcgtggcg gtcgccagcg cgaagtagcc
      361 ggcggcgatg atcagcagcg tcggcagccc gaacgcgaac agcgcataga catagtcgcc
      421 gatccggaac gggccgacct tctcgggatc gagccacggc atctgcgcgc cgatcgcgat
      481 cgccagcggg accatcgcca gcacgccgag cgcggccgcg ctggcgccgg cgaaccggcc
      541 gaccaggtag ctgaacttgc cgaccgaggt cacccggatg atcggcgcga acccggtctc
      601 gtcgtcgcgg atcaccacgc tggcgaccat cgccacgatc acgaagatcg agaacaggct
      661 caggaccgac agcgtctgca ggatcgtgta cggcgagttg acgaacacgt tgccgcggcc
      721 gccgatctgg atctggtcga tcgtcgccgc gccgaacgtg atcaggaaga acagcgcgca
      781 gccgacccag aacaccgggc tgcgcagctg gtagcgccac tcgaagccgg cgacgtgccg
      841 cagcatggcg gccctcatcg gactcccggc gccccggcga ccgcgtcccg gcggtggcgg
      901 tacagggtga cgaagtagac atcctcgaga ttgctcggca ccggctcgaa cgccggttcg
      961 ggcgcagcgt cggccagcac gcggacctgg gtccggccgg cgcgcatccg ggtcgagatc
     1021 acgccgaacc gggcgtggca cgcggcgagc tcggtcttgt cgatcgtgcg cgtccacacc
     1081 ttgccgcgga acacgtcgat cagcgcggcg ggggtgtcgt tggcgacgat ccggccgtcg
     1141 accatgaccg cgacccgcga gcacaggtcg gtgacgtcgt cgacgatgtg ggtcgacagg
     1201 atcaccacca tgttctcccc gatctcggcg agcaggttgc tgaaccggtt gcgctcctcg
     1261 gggtcgagcc cggcggtcgg ctcgtcgacg atgatcagcc ggggccggcc gatcagcgcc
     1321 tgggcgatgc cgaaccgctg ccgcatgccg ccggagaacc cggcgatcgg ccgcctgcgc
     1381 acgtgccaca ggttgacctg gtgcagcagg gtctcgacga cctcgcgccg ctcgccgcgc
     1441 gcgatcacgc ccttgagcac ggccaggtga tcgagcatgt cgtacgccga gacccgcggg
     1501 tagaccccga agtcctgcgg caggtagccc agcaccgcgc gcagccgcgc gggctcggcg
     1561 agcacgtcga tgccgtcgaa gacgatcgag ccggcggtcg ggacctgcag ggtcgccacg
     1621 cagcgcatca gggtggactt gcccgccccg ttggggccga gcagtccgaa catgccgcgc
     1681 gggatggtga gcgtgacgtc atcgagcgcg cgggtacgac cgtcgtagct gtgggacagg
     1741 ccggagatcg tgagcatgca ggctccaggt tggtggggga caggacagcc gaagcaagcg
     1801 cggtgccgcg ccgctcgcgg accacccctg gagttgacga tccgcgttgc gaaacggttc
     1861 gccaccgcgt ggtcgatcta tcgtcgcccc ggcacggccc gggcaccgtc ccggcagcgt
     1921 cgggatcccg gcgatggccg ccgcgtcctg cgccggcccc gccgcgaccg acgtcacgcc
     1981 gtgcgccggg gaaccggcgg ccgggttcac accgggtggg atggggtggt gtgtcgctgc
     2041 ggtgtcgcgg tcgcggtcgc ggtcactggc gctgcaggag cagcgagccg agcgagtacc
     2101 cggcgccgaa cgacgccatc acgccgtggc tgcccgccgg caggtcggcg ttgtggtgcg
     2161 agaacgcgat cagcgagccg gccgacgcgg tgttgccgta ctcgtcgagg atgatcggcg
     2221 cctcctcgcg ggtcgcgtcg cggccgagga tccgcttcgc gatcagctcg ttcatgctct
     2281 ggttggcctg gtgcagccag tagcgcccga tctgcgacgg atcgaggtcg tgcgcggcga
     2341 tgtggtcggc gatgaacttc gccgccagcg gcaccacgtc cttgaacacc cggcggccgt
     2401 tctggtggaa cagcttgtcg atgttgtgct cggtgctcgg atcgcagcgg tcgaggtagc
     2461 cccggttgtt gcggatgttg ctcgaccact tcgacatcat ccgggtcgac aggatctccc
     2521 acgaccccgg ccgcgcgcga tcggtcggct cgacgaccag cgacaccgag gcgtcgccga
     2581 agatgaagtg gctgtcgcgc tcgcgccagt tcatgtggcc ggtggtgagc tcggggatca
     2641 cgaccagcgc gcaccgggcc tggccgagct ggaccgcctg ggatgcgagc tgggtcgcgc
     2701 cggtcgccgc cgagcagccg agcaggatgt cgaagccgta gccgtgcgcg ccgagcgcct
     2761 cctggacctc gatcgccagc gcggggtaca tccgctgcag gttcgagcag ccgagcacca
     2821 cgaggtcgac gtcctcgccg gcgcggccgg cgacctccag cgcgcgccgc ccggcgttga
     2881 gcgcgtactc ggcctggtag ctgagctggt cctccggccg atcggggatg ttcggacaca
     2941 tccgctcggg atcgaccagg ccggtcttgt cctcgacata gcgctgctgg atgcccgacg
     3001 ccttgacgat ccactccggc gtcgactcgc gcagcggcgc gcgggtgccg gcggcgatcg
     3061 cctcggcgtt ctgcgcgttg tcgcgccgca cgaactcgtt gaacgccgcg cacagctcct
     3121 cgttgctgag caccgtcggc ggatgccaca cgccgtagcc cgtgatggat gtcctggtcg
     3181 tcgtccgggg agccatgacg cgcaccttac ccgagggctt tcgaggtcgg aaatcgcaat
     3241 gtgtcgatct tgtctcgtcc tcggtctcgt cctcggtctc ggccgtgggt ctcagcgttc
     3301 gcctcgggga ccggcctcgg tgtttccggt gcgccggtcc gaccagtcgt gaccaggtta
     3361 tggctttttc atgacctctg cttcgacctc ggcgagcgca ctgcgcagga acgccgactg
     3421 cggatcgctg gcgatcgcca tcttgaggtg gagcaccgcg ccggtcaggt cgccgcgccg
     3481 gagcgccagc tcggccttgc gataatgcac gagcgccttg ctcgacatgc gctgcgccgg
     3541 gccggccgcc tcgtcgggcg ccgcgaccgg cccgtgcgat ggcggcggag cggggatcgc
     3601 ggcggtggtc ggcacggccg g
//
LOCUS       DASVOR010001493         2005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_44718,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001493 DASVOR010000000
VERSION     DASVOR010001493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44718"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..417)
                     /locus_tag="VF516_28230"
     CDS             complement(22..417)
                     /locus_tag="VF516_28230"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEX8111664.1"
                     /translation="MANVNILAVAVAALSSLLIGGVWFSPILFAHAWQREAGLTDDQL
                     RKGAGKALGSAFVLALIIALNLGMFLGRHAGIAWGAGAGALAGIGWATASLATVYLFE
                     RRSLILIVIDGGFLAVSYAVMGAIIGVWP"
     gene            548..1546
                     /locus_tag="VF516_28235"
     CDS             548..1546
                     /locus_tag="VF516_28235"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8111665.1"
                     /translation="MRAVGIPAFGLGIPRITGLLDGVEPAGPHAAAYWIGSAAFVALA
                     AAIWHGNRWLLFEQRRHWDWFDHPVRKVVMLLAAIVLFTVPLTVATLAAWYAARGASG
                     DVATIRTVVLMIVICVVFVTHVYETVFLIKERASDRVRVAELERARAEAQLSAFLAQA
                     DPHFMFNALHALAALVETDAPRARAFTEHLADMHRYLLAQLGKTLATVADELRFLDDF
                     AELMRLRLGSGLAIAVIDDGIDRQARLPPTAIQSLVENAIKHNQLPLAIEVRLGADAV
                     VVGHDRKPRRSQRPSAGVGLRNLDECCRLATGRGLSIDQQGGRFVVAVPIVPRAAA"
     gene            1543..>2005
                     /locus_tag="VF516_28240"
     CDS             1543..>2005
                     /locus_tag="VF516_28240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8111666.1"
                     /translation="MTIVILEDEAPARAWFEQVLARHRPGAELVVLASVAEAIAYLAA
                     HPPPDLIIADIQLADGLSLEIFDHTQPGCPVIFATASDEYVIEALQRDGIAYLLKPLR
                     EPDVVGALAKYDRLESHFAERVRGVARRLGAPRRLVARRRDGFVVLSLAEVA"
BASE COUNT          269 a          747 c          714 g          275 t
ORIGIN      
        1 cgcagccggc ggagcggcgc ttcagggcca cacgccgatg atcgcgccca tcaccgcgta
       61 cgacaccgcg aggaagccgc cgtcgatcac gatcaggatc agcgagcgcc gctcgaacag
      121 gtacacggtc gcgagcgacg cagtcgccca gccgatcccg gcgagcgccc cggcgccagc
      181 gccccacgcg atgccggcgt ggcggcccag gaacatgccg aggttgagcg cgatgatcag
      241 cgcgagcacg aacgcgctgc ccagcgcctt gccggcgccc ttgcggagct ggtcgtcggt
      301 caggccggcc tcgcgctgcc acgcgtgcgc gaacaggatc gggctgaacc acacgccgcc
      361 gatcaggagg cttgacagcg cggcgaccgc gacggcgaga atgttgacgt tggccatgcc
      421 gacacgaata ccgatctcgg cgggcccgtc gccagcgatc tccgcccagg ccggagatcc
      481 ggcgaccgaa gccggccgat ccgggcgtga accggcggcc ggccccatgc ggatcgacga
      541 tcgctgggtg cgcgccgtgg gcatcccggc gttcggcctc gggatccccc ggatcaccgg
      601 gctgctcgac ggggtcgagc cggccgggcc acacgccgcc gcctactgga tcgggtcggc
      661 ggcgttcgtc gcgctggccg ccgcgatctg gcacggcaac cgctggctcc tgttcgagca
      721 gcgccggcac tgggactggt tcgatcaccc ggtccgcaag gtcgtgatgc tgctcgcggc
      781 gatcgtgctg ttcaccgtgc cgctcacggt cgcgaccctc gcggcatggt acgcggcgcg
      841 cggcgcctcc ggcgatgtcg cgacgatccg caccgtggtg ctgatgatcg tgatctgcgt
      901 ggtgttcgtg acccacgtct acgagaccgt gttcctgatc aaggagcgcg cgtcggaccg
      961 ggtccgcgtc gccgagctcg agcgcgcccg cgccgaggcc cagctgtcgg cgttcctcgc
     1021 ccaggccgat ccgcacttca tgttcaacgc gctccacgcg ctcgccgcgc tggtcgagac
     1081 cgacgcgccg cgcgcccgcg cgttcaccga gcacctcgcc gacatgcacc gctacctgct
     1141 cgcccagctc ggcaagacgc tggcgacggt cgccgacgag ctgcgcttcc tcgatgactt
     1201 cgccgagctg atgcggctgc ggctgggcag cgggctcgcg atcgcggtga tcgacgacgg
     1261 catcgaccgc caggcccggc tgccgccgac cgcgatccag agcctggtcg agaacgcgat
     1321 caagcacaac cagctgccgc tggcgatcga ggtccggctc ggcgcggacg cggtcgtggt
     1381 cggccacgat cgcaagccgc ggcgcagcca gcggccgtcg gccggcgtcg gcctgcgcaa
     1441 cctcgacgag tgctgccggc tggcgaccgg ccgcggcctc tcgatcgacc agcagggcgg
     1501 gcggttcgtg gtcgcggtgc cgatcgtccc gcgggccgcc gcatgacgat cgtgatcctc
     1561 gaggacgagg cgccggcgcg ggcgtggttc gagcaggtcc tggcgcgcca ccggccgggc
     1621 gccgagctgg tcgtgctcgc gtcggtcgcc gaggcgatcg cgtacctcgc cgcgcacccg
     1681 ccgcccgacc tgatcatcgc cgacatccag ctcgccgacg gactgtcgct cgagatcttc
     1741 gatcacaccc agcccggctg cccggtgatc ttcgcgaccg cgtccgacga gtacgtgatc
     1801 gaggcgctgc agcgcgacgg catcgcgtat ctgctcaagc cgctgcgcga gccggatgtc
     1861 gtcggcgcgc tcgccaagta cgaccggctg gagagccact tcgccgagcg cgtgcgcggc
     1921 gtggcgcgcc ggctgggggc gccgcgccgg ctggtcgcac gccgccgcga cggcttcgtc
     1981 gtgctatcgc tcgccgaggt cgcct
//
LOCUS       DASVOR010001494         8591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_190880,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001494 DASVOR010000000
VERSION     DASVOR010001494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190880"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1044)
                     /gene="ugpC"
                     /locus_tag="VF516_28245"
     CDS             complement(<1..1044)
                     /gene="ugpC"
                     /locus_tag="VF516_28245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEX8111667.1"
                     /translation="MAEQAAAQHPPAASPDQAPAAIELRGVGKSFGTTHVLSGIDLEI
                     KQGEFCVFVGPSGCGKSTLLRLIAGLESATAGDLCIDGKRVNDVAPSRRGIAMVFQSS
                     ALYPHMTVYQNLEFGLQLARTEKQEADRRIRDAAKMLQIEHLLDRLPKQLSGGQRQRV
                     AIGRALTRRPRIFLFDEPLSNLDAALRVTMRAELSKLHRQLRATMVYVTHDQVEAMTL
                     ADRLVVLNAGHVEQVGAPLDVYHRPRTRFAAGFLGSPRMNFIAGKVTELTDAGVAVEL
                     TGGGTVTAAVAAGGVQPGDAVTVGVRPEHVRIVPTGPAPLSAAIQLIENLGDHAILHL
                     DREQQDGGRVLAKV"
     gene            complement(1047..1976)
                     /locus_tag="VF516_28250"
     CDS             complement(1047..1976)
                     /locus_tag="VF516_28250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8111668.1"
                     /translation="MADPPPPRTPAPASVPLPAAIVVGTTAEASRRLTAGRALRRAGF
                     FALVAAIVLYAVFPFYYAILTSLKSGSELFRVDYFPFKWEFGNYASVFSGQPFGTNIL
                     NSLIVAVSVVALSLFLGVTAAFALARIQFRGRSTLLVTILGVSMFPQVAVLSGMFQLI
                     RALHQFNHLGALVISYMIFTLPFTVWVLTTFMRELPKELEEAAILDGATAWTIVRRVF
                     LPLMWPALVTTGLLAFIAAWNEFLFALTFTLTNDKRTVPVGIAVMSGASAHETPWGNI
                     MAASVIVTVPLVLLVLIFQRRIVSGLTAGAVKG"
     gene            complement(1978..2895)
                     /locus_tag="VF516_28255"
     CDS             complement(1978..2895)
                     /locus_tag="VF516_28255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEX8111669.1"
                     /translation="MRDTSWLAASRTRSAWLFLAPMLVLLALVAGWPLIRTIWLGFTD
                     ANTLQIGEARWLGFDNFLTRHDGHWYGVLADPVWWRAVKNTLVFTVFSVSIETVLGLG
                     VALVLNRAFPGRGIVRAAILIPWAIPTVVSARIWGWLLNDQYGFINHVLMSLGLIDHS
                     IAWTADPSMLMTSVVLVDVWKTTPFMALLILAALQMLPKECYEAALIDGTHPLKVFWR
                     VTLPLIRPALMVAVIFRAIDALRVFDVIYVLTGANERSMTMSIYARQQLFEFNQAGYG
                     SAVSTLLFFLIALATILLLVATRVRLTED"
     gene            complement(2911..4236)
                     /locus_tag="VF516_28260"
     CDS             complement(2911..4236)
                     /locus_tag="VF516_28260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8111670.1"
                     /translation="MKTLTLLCSAAVLLTGCKKDDAAKKQPTEGAAKPAPTAAPGSIE
                     IAISCGSVGKEFDVCKEGVEAWSKKTGHKATVVTSTTSSTDKLAAAQQLLGAESSDVD
                     VFSVDVVWPGILGKFFIDLKPYTKGAEAEHFPALVANGTLDGKLVAMPWFADAGLLYY
                     RKDLLDKYKESVPTTWAQLTATAKKIQDAERKAGNKDMQGFVFQGKAYEGLTCDALEW
                     VASYGGGSVVDPDGKISINNPGAIAALELAASWPGTISPTGALNAEEEEARAVFQSGN
                     SVFMRNWPYAWALAQDASSAVKDKVGVAPLPKGDGEGARPAATLGGWDLSVSKFSKHP
                     AEAADLVMYLTSAAEQKRRAIAASLNPTIPSLYSDKDIAAANPFLASMGDVLKGAVPR
                     PSTVTSGKYNQVSNAFWNAVHAVLAKEKPAKDAVAELAGKIEQIKGPKW"
     gene            complement(4456..5436)
                     /locus_tag="VF516_28265"
     CDS             complement(4456..5436)
                     /locus_tag="VF516_28265"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEX8111671.1"
                     /translation="MTKRTGRYGDPPRSDPPVYGERKGGAPLPLLTQPMIAVDVREAR
                     DVDPKQTLIDGPVPTIKLDTAALMQAKVETSVVQGKVEPTALMHGKPTVMMGPQLKPS
                     GAPPQIDLTQHHLPEDSIDRRLALLIDPDSERSAAFRVLRHHLLEIGRPQVVIVTSPQ
                     SGDGKTTTAVNLALALAECGRAKTLLVETHVRRPQLTTVFQFVPPWCFAEQLAAHRHQ
                     PTMPWSLIDIPQLWLHVAAINPRTGQTQLLDGPAFAIAMERLRLAGYDHIVVDAPPVL
                     GSADVNLMTDAADSVVFALRARRSNTRDLRRAIEQLGDSKVAGTVLLQDR"
     gene            complement(5433..6917)
                     /locus_tag="VF516_28270"
     CDS             complement(5433..6917)
                     /locus_tag="VF516_28270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111672.1"
                     /translation="MDLGRKTGRYWWLIALFAVVGGGLSLAFALTRPRSFQSWATLFY
                     QERIQSQLLSPGREEAVQRNIGDRYRELLLARHQLDQIIVDPTLDPFPQEKDPDVKID
                     KLRLAVRLEAHGANVFRIVFTDSDPERAKRVTDKLTKMLQDKDEGLRNEQAAATVAFV
                     TDQQESAREELKKREKAYTEFLAKHPEFVQDANSTTAEGASIRSLHTNKPVLTGNSRL
                     VTLERQRARIQATLETPPGAAIPAVPVPRTPERIAAEAAVQDAQRELTAARRELEDAL
                     QKFTDLHPAVVKARERVEAATQRLRQREAAVPPDVEPVVTRVNTEEDRAKLQKELQQI
                     EAQIAAEQSKTGKPADNPATDTTSWVVRLESEHSELRRSVAEQRERVQSLADSAFRAQ
                     MDANEKLAEQGGRLAVIDPAFKPARPSGPGKTIFLAAGMILFLTLGLSLAVGLAVIDD
                     RLYRRADLERLGVTVLAVIPPAIRVQRKNRKRRTTKPTTQGGAA"
     gene            complement(6944..8056)
                     /locus_tag="VF516_28275"
     CDS             complement(6944..8056)
                     /locus_tag="VF516_28275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111673.1"
                     /translation="MPAPRSNGDQWYESEQPTRRGLVAELQRIRRRTRVRPIPVLVVA
                     ALVTAGITCKMATKPVMVEAEVVIALTEGAMASRQTGMPVDQLRGYVTQVLMPDKRLL
                     EIVEKYNLFPLRKKAGSEYALESFHDLFAVQIWKNSFAYYDDEDANAARSARIGISVR
                     DTDPDRALDVAHDLAAVVIETAAAVRQQRADTVTGQVAMLRKAVGDELDKIMIDIASK
                     QEAITQARNRGDTDLAGILRIDLAALQQQQRRHEAQLQRIAVSSDALAADIVAAGLDM
                     SLAVVDENRPERPTHSGLVLVMIAAVIGTFSLVSAALVLGAFDARVHDADDVIRLGLP
                     VLGHVPGFAGDNVGSMRTRSAAPTRVPSFRRWRFHR"
     gene            complement(8060..>8591)
                     /locus_tag="VF516_28280"
     CDS             complement(8060..>8591)
                     /locus_tag="VF516_28280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8111674.1"
                     /translation="PGGPLALYVGNLKPEKGVMDLARAWEGVARDLPDARLAVVGGGP
                     LHGALEAALPTRATLVGPQPLDRVPLWMAACDVLVLPSHIEGMPNAVLEALASGRRVV
                     ATAVGGVPDLITSEVLGTLVPPHQPEALTAALTQALRTGYDARAVAEQGAHGGWEASA
                     AALHAVLAAAAGASVQ"
BASE COUNT         1219 a         2894 c         3173 g         1305 t
ORIGIN      
        1 gaccttggcg agcacgcggc cgccgtcctg ctgctcgcgg tcgaggtgga ggatcgcgtg
       61 gtcgccgagg ttctcgatca gctggatcgc ggcggacagc ggggcggggc cggtggggac
      121 gatccggacg tgctcggggc gcacgccgac ggtgaccgcg tcgccgggct ggacgccgcc
      181 ggcggcgacc gcggcggtga cggtgccgcc gccggtgagc tcgacggcga cgccggcgtc
      241 ggtgagctcg gtgaccttgc cggcgatgaa gttcatgcgc ggcgagccga ggaagccggc
      301 ggcgaaccgg gtgcgcgggc ggtggtagac gtcgagcggc gcgccgacct gctcgacgtg
      361 accggcgttg agcacgacca ggcggtcggc gagggtcatg gcctcgacct gatcgtgggt
      421 gacgtagacc atggtggcgc ggagctggcg gtggagcttg ctgagctcgg cgcgcatggt
      481 gacgcgcagc gcggcgtcga ggttggacag cggctcgtcg aacaggaaga tccgcggccg
      541 gcgggtcagc gcgcggccga tcgcgacgcg ctggcgctgg ccgcccgaca gctgcttggg
      601 caggcggtcg agcaggtgct cgatctgcag catcttcgcg gcgtcgcgga tccggcggtc
      661 ggcctcctgc ttctcggtcc gggcgagctg gaggccgaac tcgaggttct ggtagaccgt
      721 catgtgcgga tacagcgcgg aggactggaa caccatggcg atgccgcgcc gggacggggc
      781 gacgtcgttg acccgcttgc cgtcgatgca gaggtcgccg gcggtcgcgc tctcgaggcc
      841 ggcgatcagg cgcagcaggg tcgacttgcc gcagcccgac gggccgacga acacgcagaa
      901 ctcgccctgc ttgatctcga ggtcgatgcc ggacaggacg tgggtggtgc cgaacgactt
      961 gccgacgccg cggagctcga tcgcggcggg ggcctggtcg ggggacgcgg cgggcgggtg
     1021 ctgggcggct gcttgctcgg ccacggttag ccctttactg cgccggcggt caggccggac
     1081 acgatcctgc gctggaagat caggacgagg aggaccagcg gcacggtgac gatcaccgag
     1141 gcggccatga tgttgcccca cggcgtctcg tgcgccgacg cgccgctcat caccgcgatg
     1201 ccgaccggca cggtgcgctt gtcgttggtc agcgtgaagg tcagcgcgaa caggaactcg
     1261 ttccacgcgg cgatgaacgc gagcaggccg gtggtgacca gcgccggcca catcagcggc
     1321 aggaacacgc gccgcacgat cgtccacgcg gtcgcgccgt cgaggatcgc ggcctcctcg
     1381 agctccttgg gcagctcgcg catgaacgtg gtcagcaccc agaccgtgaa cggcagggtg
     1441 aagatcatgt acgagatcac cagcgcgccg aggtggttga actggtgcag ggcgcggatc
     1501 agctggaaca tgcccgacag caccgcgacc tgcgggaaca tcgagacccc gaggatggtg
     1561 accagcagcg tgctgcggcc gcggaactgg atccgcgcca gcgcgaacgc ggcggtgacg
     1621 ccgaggaaca gcgacagcgc gacgaccgag accgcgacga tcagcgagtt gaggatgttg
     1681 gtgccgaacg gctggccgga gaacaccgag gcgtagttgc cgaactccca cttgaacggg
     1741 aagtaatcga cgcggaacag ctcgctgccc gacttgagcg aggtcaggat cgcgtaatag
     1801 aacgggaaca ccgcgtacag cacgatcgcg gcgaccagcg cgaagaaccc ggcgcggcgc
     1861 agcgcgcggc ccgcggtcag tcgccgggac gcctcggcgg tggttccgac gacgatcgcg
     1921 gcgggcagcg ggacgctggc cggggccggg gtccgaggcg gaggagggtc tgccatgtca
     1981 gtcctcggtc agccggacgc gggtcgcgac cagcagcagg atggtggcca gcgcgatgag
     2041 gaagaacaag agggtcgaga ccgccgagcc gtagccggcc tggttgaact cgaacagctg
     2101 ctggcgggcg tagatcgaca tcgtcatcga gcgctcgttg gcgccggtca ggacgtagat
     2161 cacgtcgaac acgcgcagcg cgtcgatcgc gcggaagatc accgcgacca tcagcgccgg
     2221 gcggatcagc ggcagggtga cgcgccagaa caccttgagc gggtgggtgc cgtcgatcag
     2281 cgcggcctcg tagcactcct tgggcagcat ctgcagcgcc gcgaggatca agagcgccat
     2341 gaacggcgtg gtcttccaca cgtcgaccag gaccaccgag gtcatcagca tgctgggatc
     2401 cgcggtccac gcgatcgagt ggtcgatcag gccgagcgac atcagcacgt ggttgatgaa
     2461 gccgtactgg tcgttgagca gccagcccca gatcctcgcc gacaccacgg tcgggatcgc
     2521 ccacgggatc aggatcgcgg cgcgcacgat gccgcggccg gggaacgcgc ggttgagcac
     2581 cagcgcgacg ccgaggccga gcacggtctc gatcgacacc gagaacacgg tgaacaccag
     2641 cgtgttcttg accgcgcgcc accacaccgg atcggcgagc acgccgtacc agtggccgtc
     2701 gtgtcgggtc aggaagttgt cgaacccgag ccacctggcc tcgccgatct gcagcgtgtt
     2761 ggcgtcggtg aagcccagcc agatggtccg gatcagcggc cagccggcga ccagcgcgag
     2821 cagcaccagc atcggcgcca ggaacagcca cgccgagcgc gtccggctag cggcgagcca
     2881 cgaggtgtcg cgcatcggcg acctacgagc tcaccacttg gggcccttga tctgctcgat
     2941 cttgccggcg agctcggcga ccgcgtcctt ggccggcttc tccttggcga gcaccgcgtg
     3001 caccgcgttc cagaacgcgt tgctgacctg gttgtacttg ccgctggtga ccgtcgacgg
     3061 ccgcggcacc gcgcccttga gcacgtcgcc catgctggcg aggaacgggt tggcggcggc
     3121 gatgtccttg tcgctgtaca ggctcgggat cgtcgggttg aggctggcgg cgatcgcgcg
     3181 gcgcttctgc tccgccgccg aggtcaggta catcacgagg tcggcggcct cggccgggtg
     3241 cttggagaac ttggacaccg acaggtccca gccgccgagc gtggccgccg ggcgcgcgcc
     3301 ctcgccgtcg cccttgggca gcggcgcgac gccgaccttg tccttgaccg cgctcgacgc
     3361 gtcctgggcc agcgcccacg cgtacggcca gttgcgcatg aacaccgagt tgcccgactg
     3421 gaacaccgcg cgcgcctcct cctcctcggc gttgagcgcg ccggtcggcg agatcgtgcc
     3481 gggccacgac gcggcgagct cgagcgccgc gatcgcgccg gggttgttga tcgagatctt
     3541 gccgtcgggg tcgaccacgc tgccgccgcc gtagctggcg acccactcga gcgcgtcgca
     3601 ggtcaggccc tcgtaggcct tgccctggaa cacgaagccc tgcatgtcct tgttgccggc
     3661 cttgcgctcg gcgtcctgga tcttcttggc ggtcgcggtg agctgggccc aggtcgtggg
     3721 caccgactcc ttgtacttgt cgagcaggtc cttgcggtaa tacaagaggc ccgcgtcggc
     3781 gaaccacggc atcgcgacca gcttgccgtc gagcgtgccg ttggcgacca gcgccgggaa
     3841 gtgctcggcc tcggcgccct tggtgtacgg cttgaggtcg atgaagaact tgcccaggat
     3901 gccgggccac accacgtcga cgctgaacac gtcgacgtcc gacgactcgg cgccgagcag
     3961 ctgctgcgcc gccgcgagct tgtcggtcga cgacgtggtc gacgtcacca cggtcgcctt
     4021 gtggccggtc ttcttggacc acgcctcgac gccctccttg cagacgtcga actccttgcc
     4081 gaccgagccg cacgagatcg cgatctcgat cgacccgggc gcggcggtgg gcgccggctt
     4141 ggcggctccc tcggtcggct gcttcttggc agcgtcatcc ttcttgcagc cggtgagcag
     4201 cacggccgcc gagcacagca aggtgagtgt cttcatcacg cgacctctag cactggccgt
     4261 gaggcggtcg caacgcaccg tacctaccgt tctgtcaccg gcgctgacct ggccggtgag
     4321 gggtctcgta cggactctga ggtgcgataa cccgcgcgac gcgacgcgcg aaggggcgac
     4381 cggaggggtg aatggaccgg ctttgccgcg aaagacggga cgggaaggtc ccctctggat
     4441 agatgtcgcg gtgcgtcagc ggtcctggag caggaccgtg ccggcgacct tgctgtcgcc
     4501 gagttgctcg atcgcgcgcc gcaggtcgcg cgtgttggac cgccgcgcgc gcagcgcgaa
     4561 caccacgctg tcggccgcgt cggtcatcag gttgacgtcg gccgagccga gcaccggcgg
     4621 ggcgtcgacc acgatgtggt cgtagccggc cagccgcagc cgctccatcg cgatcgcgaa
     4681 cgccggaccg tcgagcagct gggtctgacc ggtccggggg ttgatcgccg cgacgtgcag
     4741 ccacagctgc gggatgtcga tcaggctcca cggcatggtc ggctggtggc ggtgggcggc
     4801 gagctgctcg gcgaagcacc acggcgggac gaactggaac acggtggtga gctggggccg
     4861 gcggacgtgg gtctcgacca gcaaggtctt ggcccggccg cactcggcga gcgcgagcgc
     4921 gaggttgacc gcggtcgtgg tcttgccgtc gccgctctgc gggctggtca cgatcacgac
     4981 ctgcggccgg ccgatctcga gcaggtggtg gcgcagcacc cggaacgccg cgctgcgctc
     5041 ggagtccgga tcgatcagca gcgcgagccg ccggtcgatg ctgtcctcgg ggaggtggtg
     5101 ctgggtcagg tcgatctgcg gcggcgcgcc cgacggcttg agctgcgggc ccatcatcac
     5161 ggtcggcttg ccgtgcatca gggccgtcgg ctcgaccttg ccctgcacga ccgaggtctc
     5221 gaccttggcc tgcatcagcg cggcggtgtc gagcttgatc gtcggcaccg ggccgtcgat
     5281 cagcgtctgc ttgggatcga cgtcgcgcgc ctcgcggacg tcgaccgcga tcatcggctg
     5341 ggtcagcagc ggcagcggag cgccaccctt gcgctcgccg tacaccgggg gatcggatcg
     5401 gggtggatcg ccatatcggc cggtgcgctt ggtcacgcgg cgcctccttg ggtcgtcggc
     5461 ttcgtcgttc gccgcttgcg gttcttgcgt tggacccgga tcgccggcgg gatcacggcg
     5521 agcacggtga cgccgagccg ctcgaggtcg gcgcggcggt acagccggtc gtcgatcacc
     5581 gcgagcccga ccgccagcga caggccgagg gtcaggaaca ggatcatgcc ggcggccagg
     5641 aagatcgtct tgccggggcc gctcggccgc gccggcttga acgccgggtc gatcacggcg
     5701 agccggccgc cctgctcggc gagcttctcg ttggcgtcca tctgggcgcg gaacgcgctg
     5761 tcggcgagcg actggacccg ctcgcgctgc tcggcgaccg agcggcgcag ctcgctgtgc
     5821 tcgctctcga gccggaccac ccagctggtg gtgtcggtcg cggggttgtc ggccggcttg
     5881 ccggtcttgg actgctcggc ggcgatctgg gcctcgatct gctggagctc cttctgcagc
     5941 ttggcgcgat cctcctcggt gttgacccga gtgaccaccg gctcgacgtc gggcgggacc
     6001 gcggcctcgc gctggcgcag ccgctgggtc gcggcctcga cgcgctcgcg ggccttgacc
     6061 acggccgggt ggaggtcggt gaacttttgc agcgcatcct cgagctcgcg cctggcggcg
     6121 gtgagctcgc gctgcgcgtc ctggaccgcg gcctcggcgg cgatccgctc gggggtgcgc
     6181 ggcaccggga ctgccgggat cgccgcgccg ggtggcgtct cgagggtggc ctggatccgg
     6241 gcgcgctggc gctcgagcgt gaccagccgc gagttgcccg tgaggaccgg cttgttggtg
     6301 tggagcgagc ggatcgacgc gccctcggcc gtggtgctgt tggcgtcctg cacgaactcg
     6361 gggtgcttgg cgaggaactc ggtgtacgcc ttctcgcgct tcttgagctc ctcgcgcgcg
     6421 ctctcctgct ggtcggtgac gaacgcgacg gtcgcggcgg cctgctcgtt gcgcaggccc
     6481 tcgtccttgt cctgcagcat cttggtcagc ttgtcggtga cccgcttggc gcgctcggga
     6541 tcgctgtcgg tgaacacgat gcggaacacg ttggcgccgt gcgcctcgag ccggaccgcg
     6601 agccgcagct tgtcgatctt gacgtcgggg tccttctcct gcgggaacgg gtcgagcgtc
     6661 gggtcgacga tgatctgatc gagctggtgg cgcgccagca ggagctcgcg atagcggtcg
     6721 ccgatgttgc gctggacggc ctcctcgcgg cccggcgaca ggagctggga ctggatacgc
     6781 tcctggtaga acagcgtcgc ccacgactgg aagctgcgcg gccgggtcag cgcgaacgcg
     6841 agcgacagcc cgccgccgac cacggcgaac agcgcgatga gccaccagta gcgcccggtc
     6901 ttgcgaccga ggtccatcag gcgttgcacg tggtcgcgtg gggttatcgg tggaagcgcc
     6961 atctccgaaa cgatggtaca cgagtgggtg cggcgctacg tgtccgcatg gagccgacgt
     7021 tgtcgcctgc aaatcccggc acatgcccga gcacgggcag cccgagccgg atcacgtcgt
     7081 cggcgtcgtg aacccgggca tcgaacgcgc cgagcacgag cgcggcgctg accagcgaga
     7141 atgttccaat cacagcggca atcatcacca gcaccagccc cgagtgggtc gggcgctccg
     7201 gccggttctc gtcgaccacc gccagcgaca tgtcgagtcc ggcggcgacg atgtcggcgg
     7261 ccagcgcgtc cgagctcacc gcgatccgct ggagctgggc ctcgtggcgc cgctgctgct
     7321 gctgcagcgc ggcgaggtcg atccgcagga tcccggcgag gtcggtgtcg ccgcggttgc
     7381 gcgcctgggt gatcgcctcc tgcttgctcg cgatgtcgat catgatcttg tcgagctcgt
     7441 cgcccaccgc cttgcgcagc atcgcgacct ggccggtcac ggtgtcggcg cgctgctggc
     7501 ggacggcggc cgcggtctcg atcacgaccg cggcgaggtc gtgcgcgacg tcgagcgcgc
     7561 gatccggatc ggtgtcgcgc accgagatcc cgatccgcgc cgagcgcgcc gcgttggcgt
     7621 cctcgtcgtc gtagtacgcg aacgagttct tccagatctg gaccgcgaac aggtcgtgga
     7681 agctctcgag cgcgtactcc gagccggcct tcttgcgcag cggaaacagg ttgtacttct
     7741 cgacgatctc gagcagccgc ttgtccggca tcaggacctg ggtgacgtag ccgcgcagct
     7801 ggtcgaccgg catgccggtc tggcggctcg ccatcgcgcc ctcggtcagc gcgatcacca
     7861 cctcggcctc gaccatgacc ggcttggtcg ccatcttgca cgtgatcccg gcggtgacca
     7921 gcgcggcgac cacgagcacc gggatcggcc ggacccgggt ccggcggcgg atccgctgca
     7981 gctcggcgac cagcccgcgg cgcgtcggct gctccgactc gtaccactgg tctccgttgg
     8041 agcgtggagc gggcatcgcc tactgcacgc tagcacccgc ggcggcggcc agcacggcgt
     8101 gcagcgccgc ggcgctggcc tcccagccac cgtgcgcacc ctgctcggcc acggctctgg
     8161 cgtcatagcc ggtgcgcaag gcctgcgtga gggcagcggt cagcgcctcg ggctggtgcg
     8221 gtgggaccag cgtgccgagc acctcgctcg tgatcaggtc gggcacgccg ccgacggcag
     8281 tcgccaccac ccgccgcccc gaggccagcg cctccagcac ggcgttcggc atgccctcga
     8341 tgtggctggg cagcacgagc acgtcgcacg ccgccatcca cagcggcacc cggtcgagcg
     8401 gctgggggcc gaccagggtc gcgcgcgtcg ggagtgcggc ctccagcgcc ccgtgcagcg
     8461 gcccgccgcc gacgaccgcc agccgggcgt ccggcaggtc gcgcgccacc ccctcccacg
     8521 cccgggcgag atccatcacg cccttctcgg gcttgaggtt gccgacgtag agcgccagcg
     8581 ggccgccggg c
//
LOCUS       DASVOR010001495         4270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_482980,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001495 DASVOR010000000
VERSION     DASVOR010001495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4270
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482980"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(359..2137)
                     /locus_tag="VF516_28285"
     CDS             complement(359..2137)
                     /locus_tag="VF516_28285"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8111675.1"
                     /translation="MTRIQKRILSGFLSYELDLATQFSVQTLRNYQPRGNEECPVRRG
                     SNIKVNQNCLNVTDPDLQGRAQAENEPAIAADPNQPDHLVASANDYLRGDGTCGSYWS
                     TDGGRTWNDSTLPNAFVRGTRFGAEREYFQACGDTAVAWDSKGNAYELAMMFQRGTPV
                     TNNHDFSSGIYVFRSTGNQGASWNFPGRPAAELANMTTGNVLLDKPYMTVDSWPSSPY
                     QDRVYVTWSWFDVDGTVYIYGAHSSDYGQTFSGPVVVSTNSPLCGNTYNLPTPHGACN
                     ENQFSQPFTSPDGTLYVVYNNYNNVTTGNDNRNQVLLVKSTDGGRTFGAPVKVADFYD
                     LPDCFTYQGQDPGRSCVPEKGPSKNSFFRAANYPSGAVDPKNPSRVAITLGSYINVHS
                     NEGNGCIPSGVSAASGLNLYIGVKQPGACNNDILLSVSRDGGRRFTGTTTDPRQLPSV
                     TSAPDQATTDQWFQWAAFTRSGKLAVSYYDRQYDNDEMTGFSDFSLSGSDDLRTFGVQ
                     RVTSASMPPPTQFDGLFWGDYTGLTAVHEAHPIWSDTRDLDVFICQDASGKVTLPPSL
                     CLASAPNARLANDQDLYTTGLGVPVH"
     gene            2513..3172
                     /locus_tag="VF516_28290"
     CDS             2513..3172
                     /locus_tag="VF516_28290"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEX8111676.1"
                     /translation="MTRCDGWQWSYSSDDVHAAGRGGGRGGPHRRGPWGGGPGGSWGG
                     GPGGPWGGPPWASFLQAIFGLDRGPRARRGDVRAAILVLLDEEPRNGYQLMQELERRS
                     QGAWRPSSGSVYPTLSQLEDEGLVAATKDESNRVYRLTERGKSYVAKHRDELGTPWEV
                     ETSSHDPRWELMNLMREIGPALGQVMKFGTPEQVAEARRIMTEARRALYRLLADDEAE
                     E"
     gene            3320..>4270
                     /locus_tag="VF516_28295"
     CDS             3320..>4270
                     /locus_tag="VF516_28295"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111677.1"
                     /translation="MTRNPTRIGRYDLITALATGGMGRIWLGRATGVGGFERKVVIKT
                     LELPRDTAEAEAASAMFLDEARLLGLLHHQHLASVFEVGQDDGRYYMVIDYLDGHSAH
                     DVWERASQYGAALPLDFTLTVASAVANGLHYAHTRRGLDGAPLGIVHRDVTPSNVMIG
                     YDGAVQLIDFGIAMAAERKAKTQTGLVKGKVSYLSPEQVTGRDVDARTDVFALGILLY
                     EMSTMHRAFRDSSDLATMQRIKAGRVTRPSLVVPNYPLELEIIVMKALSVDPRERFGD
                     ADAMRRALEALGHRLRLVLGDAAIIEVMAQLFDTRAEADEA"
BASE COUNT          683 a         1351 c         1525 g          711 t
ORIGIN      
        1 ggacccgggc agaccgtggg gcggtggccc gagcgggccg tggggtgggc gggccgcggg
       61 gcgagcttcc cgcaggcgat cttcggcctg ggccgcggcg cggcgccgcg acgtgcgcgc
      121 cgcgatcctg gcatcgctcg acgaggatcc gcgcaacggc ctgcccgcgg atgcaggagc
      181 tcgagcggcg cagccagggc gcgcggcggc cgggctcggg cttcacacac cccgagggct
      241 cgcgatccga gcggacagga atgactcctg ccgggaccac gaggaccgtt gcagggacgg
      301 tctgctggcg ggcatcgttc cgggcacgac acccgatcgg tgccgccgcc gaaggcggtc
      361 aatgcacagg cacgccgaga cccgtcgtgt agagatcctg gtcgttcgcg aggcgcgcgt
      421 tgggggcgct ggccaggcac agcgatggcg gcaacgtcac cttgccgctg gcatcctggc
      481 agatgaacac atccagatcg cgggtgtcgc tccagatcgg atgagcttcg tggacggcgg
      541 tcaggccggt gtaatcaccc cagaacagac cgtcgaattg cgtgggcggc ggcatcgacg
      601 ccgaggtcac gcgttgcacg ccgaacgtgc gcaggtcatc cgaacccgat agactgaaat
      661 cggagaagcc ggtcatctcg tcattgtcat actggcggtc gtaatacgag acggcgagct
      721 tgcccgatct ggtgaatgct gcccactgga accactggtc ggtcgtggcc tggtcggggg
      781 ccgatgtcac gctgggcaac tgccgcggat cggtggtcgt gccggtgaac ctccggccgc
      841 cgtccctcga cacgctgagc aagatgtcgt tgttgcacgc gcccggctgc ttcacgccga
      901 tgtacaggtt gagtcccgac gctgccgaca cgccggacgg gatgcatccg ttaccttcgt
      961 tggagtgcac gttgatgtac gagcccagcg tgatggcgac gcggctcggg ttcttggggt
     1021 cgacggcgcc cgacgggtag ttggccgcgc ggaagaacga gttcttgctc ggccccttct
     1081 ccggcacgca cgaccgtccg ggatcttgac cctgataggt gaagcaatcg ggcagatcgt
     1141 agaaatccgc caccttgacc ggcgcaccga atgtccggcc gccgtccgtg gacttcacga
     1201 gcagcacctg gttgcggttg tcgttgccgg tcgtcacatt gttgtagttg ttgtacacca
     1261 catagagcgt accgtcgggg ctggtgaatg gctgtgagaa ctggttttca ttgcaagcgc
     1321 cgtgtggggt cggcaggtta tatgtattgc cacacagtgg gctgttggtg ctgaccacta
     1381 cagggccact gaaggtctgg ccgtaatcgg aggagtgggc tccgtaaatg tacaccgtgc
     1441 cgtccacgtc gaaccagctc cacgtcacgt agacccggtc ctggtacggg ctcgacggcc
     1501 agctgtcgac ggtcatgtag ggcttgtcga gcagaacgtt gccggtggtc atgttggcca
     1561 gctccgcggc gggacggccg gggaagttcc acgaggcgcc ctggttgccg gtcgaccgga
     1621 acacgtagat gccgctcgag aagtcgtggt tgttggtgac gggggtgcca cgctggaaca
     1681 tcatggccag ctcgtaggcg ttgcccttgc tgtcccaggc cacggcggtg tcgccacacg
     1741 cctgaaagta ctcacgctcc gcaccgaacc tggtgccgcg gacgaatgcg ttcggcagcg
     1801 tgctgtcatt ccacgtccgc ccgccgtcgg tgctccagta gctgccacag gtaccatccc
     1861 cacgcaggta gtcgttggcg ctggccacga ggtggtcggg ttgattggga tccgcggcga
     1921 tcgccggctc gttctcggcc tgagctcggc cctggaggtc cggatcggtc acgttcaggc
     1981 agttctgatt caccttgatg ttggagcccc ggcggacagg gcattcctcg ttgccgcgag
     2041 gctggtaatt cctgagtgtc tgcacgctga actgagtggc aagatccagc tcgtatgaca
     2101 ggaacccaga taggatacgt ttctgtatcc tggtcatgtg accgagagta ggatgtggtg
     2161 catcgcccgt cgggctggcg ggtgtgcacg tcacggatcc gccggcgatc gggatgacca
     2221 gcgctgccag aacacgaagt ctcatgacct tccccttcct ctctacgccc acaacagccc
     2281 tgcagatgat cgatggctac cgtgcttgag gtcaggatga cctcaacacg gtagcccgtc
     2341 gacagagatc tgcgtgaggc aatgcccgag gtgccggccg agccgatcac cgccatcgca
     2401 gccccagcgt gagcgtgtgc gcgcgctgcg ggccggctcc ggtcagcgcg cggggtttcg
     2461 gagcgacgat gccccttgac atgtcacgat atatcgttac catgtcgtta ccatgacgcg
     2521 atgtgatgga tggcaatggt cctacagcag cgacgacgtt catgccgcgg ggcgcggtgg
     2581 tgggcgcggc ggtccacacc ggcgcggccc gtggggggga ggcccgggtg ggtcgtgggg
     2641 cggtggtcca ggcgggccat ggggcgggcc gccgtgggcg agcttcctgc aggcgatctt
     2701 cggcctggat cgcgggccgc gggcgcggcg cggcgacgtg cgcgcggcga tcctggtgct
     2761 gctcgacgag gagccgcgca acggctacca gctgatgcag gagctcgagc ggcgcagcca
     2821 gggcgcgtgg cggccgagct cgggctcggt gtacccgacg ctgtcgcagc tcgaggacga
     2881 ggggctggtc gcggcgacca aggacgagtc gaaccgggtc taccggctga ccgagcgcgg
     2941 caagagctac gtcgccaagc accgcgacga gctcggcacg ccgtgggagg tcgagacctc
     3001 gagtcacgat ccgcgctggg agctgatgaa cctgatgcgc gagatcggtc cggcgctggg
     3061 acaggtgatg aagttcggga cgcccgagca ggtcgccgag gcgcgccgga tcatgaccga
     3121 ggcgcggcgc gcgctgtacc ggctgctcgc cgacgacgaa gccgaggagt gatcgaggag
     3181 cgcgaacgat cctcaataga tctgtcctcg gtggcaggcc tggtgacctc ggcgccgtgc
     3241 acctacgcgc gctcaccagg tacgcagagt gtgcgatcca ggccccatcg ccgggcacaa
     3301 gccgttatgc tgacttgtgg tgacgcggaa tccgacccgc atcggccgct acgatctcat
     3361 caccgccctg gcgacgggcg gcatggggcg gatctggctg ggccgggcga cgggcgtcgg
     3421 cgggttcgag cgcaaggtcg tgatcaagac cctggagctg ccgcgcgaca ccgccgaggc
     3481 cgaagctgct tcggcgatgt tcctcgatga ggcccgtttg ctcggcctct tgcatcacca
     3541 gcacctcgcc agcgtgttcg aggtcggcca ggacgacggc cgctactaca tggtgatcga
     3601 ctacctcgac ggccacagcg cgcacgacgt gtgggagcgc gcgagccagt acggcgcggc
     3661 gctgccgctc gacttcaccc tgaccgtggc ctcggcggtc gccaacggcc tgcactacgc
     3721 gcacacccgc aggggcctcg acggggcgcc gctcggcatc gtgcaccgcg acgtcacgcc
     3781 gtccaacgtg atgatcggct acgacggcgc ggtccagctg atcgacttcg ggatcgcgat
     3841 ggcggcggag cgcaaggcca agacccagac cgggctggtc aagggcaagg tcagctacct
     3901 gtcgcccgag caggtcaccg gccgcgacgt cgacgcgcgc accgacgtgt tcgcgctcgg
     3961 catcctgctg tacgagatgt cgacgatgca ccgcgcattt cgcgacagct cggacctcgc
     4021 gacgatgcag cggatcaagg ccggccgggt gacgcggccg tcgctggtcg tgccgaacta
     4081 tccgctcgag ctcgagatca tcgtgatgaa ggcgctgtcg gtcgacccgc gcgagcggtt
     4141 cggcgacgcc gatgcgatgc gccgcgcgct cgaggcgctc ggccaccgcc tccgcctggt
     4201 gctcggcgac gccgcgatca tcgaggtcat ggcgcagctg ttcgacaccc gcgccgaggc
     4261 ggacgaggcg
//
LOCUS       DASVOR010001496         4265 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_365989,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001496 DASVOR010000000
VERSION     DASVOR010001496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4265
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365989"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /locus_tag="VF516_28300"
     CDS             complement(<1..1239)
                     /locus_tag="VF516_28300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8111678.1"
                     /translation="MTSEPTPSSTTPPPAIRWLSPINIVLFLLCVMYFLTYVDRVNIL
                     TAATDIQKEFGLNKKEYGFAFSAFGYPYLLFQVIGGRIGDRFGPRRTLFVCGAIWAVA
                     TALTGLAGGLMSLFLFRVVLGFGEGATFPTATRAMQNWTPASWRGFAQGITHSFARLG
                     NAVTPPIVAALMTWRSWRAAFVVLGVGSMIWVIVWWLYFRDDPRDHGGVAPEDLARLP
                     PPAAVKARPEVPWRRLITRMLPVTFTYFCYGWSLWVFLTWIPQFFRENADIHLDLKKS
                     ALYSSGVFFAGVIGDTLGGVISDHLLRRTGRVTFSRLVVIVIGMLGAAGCLVPVVLTT
                     DLNTLALCLSGAFFFLELIIGPIWAVPMDIAPQHAGTASGLMNSGSALAAIVSPPVFG
                     AILDATNNNWHLAFLTSVVFL"
     gene            complement(1236..2453)
                     /locus_tag="VF516_28305"
     CDS             complement(1236..2453)
                     /locus_tag="VF516_28305"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8111679.1"
                     /translation="MTQSLAKTASATRSGRHFLQIPGPTNVPDRVLRAMDRPTIDHRG
                     PEFARLATEIFDGLRTVFATSGPVIVFPSSGTGAWEAAIVNTLRPGDRVLMFETGHFA
                     LLWKKIAERLGLVVDFVPGDWRRGADPRAVEDRLTEDRAGAIKAVMVVHNETSTGVTS
                     RIPEIRRAIDRAGHDALLMVDTISSLGSIDVRHAEWGADVTVAGSQKGLMLPPGLGMN
                     AISDKALAAARQGGMPRSYWDWEEMLKHNASGFFPYTPATNLLYGLREALAMMREQGM
                     PAVFARHNRLAEATRRAVRAWGLDIVCADPAEYSSVLTAVVMPDGHDADAFRATVLAR
                     FDMSLGMGLSRLAGKVFRIGHVGDLNDLTLLGALAGVEMGLAICGVPHRPGGTQEAMA
                     FLRENAPHEGSAR"
     gene            2594..>4265
                     /locus_tag="VF516_28310"
     CDS             2594..>4265
                     /locus_tag="VF516_28310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111680.1"
                     /translation="MIGTAGLALAALAIHSSPVSGFTQSGVTINFIGSGIPMGHEWIT
                     RMAGMELLGGDPVIKTDPNDPRKHWTQGLPKDPDIKNASAEVKRIKDHKVKDARYEST
                     YEAVYAAIIGERWVDIAGFNVTKSQIGSGPNCFDNVAQEPVDVQYDHFMRRYDDRDGG
                     GGVDAAQRSRDRFVDYFVTAATAPHGVITVWDGGGYSALVTVDRNYFLFGRAVHLFED
                     SFSSEHTVRLESDMYEKVRQVKSYLCAAGSEQHTHSQKEVFDYQSGDVIWKPGTRFDA
                     GWSGYKASNMKTVALVGTDGMIDMWAAFIRTMNKDDKDRADAARKEANRLIDSWLNFD
                     KDEVKNWYNDASHRDDTYVLGNGQSGKGQTVKQCMAGLKVGTDDQDKYAKQIEDNRRY
                     CLYNIVPVLGFSDAQDPELKMPYNWQWRDSLHWQTPPKDWKIPDVKVPPEIKVTIKSV
                     KNKDYMVAPDGVAKDSWIYCKNGDALKWVVVGNKDSAIFRLDSAPLFLSYRLTTGAVK
                     LYDTATDAEYKIGKQDGKRTIYNLRYKDYMWLDDKSPYVSSKGDPKKDS"
BASE COUNT          806 a         1475 c         1402 g          582 t
ORIGIN      
        1 caggaagacg accgacgtga ggaacgccag gtgccagttg ttgttggtgg catcgagaat
       61 ggcgccgaac accggcggtg acacgatcgc cgccagcgcc gagccgctgt tcatgagccc
      121 gctcgccgtc ccggcgtgct gcggcgcgat atccatcggc accgcccaga tcgggccgat
      181 gatgagctcg aggaagaaga acgcgccgga caggcacagc gcgagggtat tcagatcggt
      241 cgtcagcacc accgggacca gacagccggc ggcgccgagc atgccgatca cgatcaccac
      301 gagtcgcgag aaggtgaccc ggccggtgcg acgcagcaga tggtcgctga tcacgcctcc
      361 cagcgtgtcc ccgatgacac cagcgaagaa cacgccgctc gagtacagcg cggacttctt
      421 gagatcgagg tggatgtcgg cgttctcgcg aaagaactgc ggtatccagg tcaggaacac
      481 ccacagggac cagccgtagc agaagtacgt gaacgtcacg ggtagcatgc gcgtgatcag
      541 ccgtcgccac ggcacctccg gcctcgcctt caccgcagcc ggcggcggca accgcgcgag
      601 atcctccggc gcgacaccgc cgtgatcgcg cgggtcgtcg cggaagtaca gccaccacac
      661 gatgacccag atcatgctgc cgacgccgag cacgacgaac gcagcgcgcc agctccgcca
      721 ggtcatgagc gccgccacga tcggaggcgt caccgcgttc ccgaggcggg caaacgagtg
      781 ggtgatcccc tgcgcgaagc cgcgccagga cgccggcgtc cagttctgca tcgcgcgggt
      841 cgcggtcggg aacgtggcgc cctcgccgaa gccgagcacg acgcggaaca ggaacagcga
      901 catcagtccg cctgcgagcc cggtgagcgc ggtcgccacc gcccagatcg cgccgcacac
      961 gaacagcgtc ctgcgcgggc cgaaccgatc gccgatccgc ccaccgatca cctggaagag
     1021 caaataggga tagccgaacg ccgagaacgc gaatccgtac tccttcttgt tgagaccgaa
     1081 ctccttctgg atatcggtcg cggccgtcaa gatgttgacc cggtcgacgt aggtcaggaa
     1141 gtacatcacg cacagcagga acagcacgat gttgatcggg ctcagccacc ggatcgccgg
     1201 gggcggggtc gtcgagctgg gcgtcggttc gctggtcatc gcgcgctccc ttcgtgcggg
     1261 gcattctctc gcaggaacgc catcgcctcc tgcgtgccgc cggggcggtg cgggacgccg
     1321 cagatcgcca ggcccatctc gacgccggcg agcgcgccga gcagcgtcag gtcgttgagg
     1381 tcgccgacgt gcccgatgcg gaacaccttg ccggcgagcc tggacagccc catcccgagc
     1441 gacatgtcga accgcgccag cacggtggcg cggaacgcgt ccgcgtcgtg gccgtccggc
     1501 atcaccaccg cggtcagcac gctggagtac tccgccggat cggcgcacac gatgtcgagg
     1561 ccccacgcgc gcaccgcgcg gcgggtcgcc tcggccagcc ggttgtggcg ggcgaacacc
     1621 gccggcatgc cctgctcgcg catcatcgcg agcgcctcgc gcaggccgta cagcaggttg
     1681 gtcgccggcg tgtacgggaa gaacccgctg gcgttgtgct tgagcatctc ctcccagtcc
     1741 cagtacgagc gcggcatgcc gccctgacgc gccgcggcga gcgccttgtc gctgatcgcg
     1801 ttcatcccca gccccggcgg cagcatcagg cccttctgcg agcccgccac cgtgacgtct
     1861 gcgccccact ccgcgtggcg cacgtcgatc gaccccagcg aggagatcgt gtcgaccatc
     1921 agcagcgcgt cgtgccccgc gcggtcgatc gcccgccgga tctcggggat ccggctggtg
     1981 acgccggtcg aggtctcgtt gtggacgacc atcaccgcct tgatcgcgcc cgcgcggtcc
     2041 tcggtcagcc tgtcctcgac cgcgcggggg tcggcgccgc gccgccagtc gccggggacg
     2101 aagtcgacca ccaggccgag ccgctcggcg atcttcttcc acaacagcgc gaagtgcccg
     2161 gtctcgaaca tcagcacccg gtcgcccggt cgcagcgtgt tgacgatcgc cgcctcccac
     2221 gcgccggtgc ccgacgacgg aaacacgatc accggccccg aggtcgcgaa cacggtgcgc
     2281 agcccgtcga agatctcggt ggccagccgc gcaaactcgg ggccgcggtg gtcgatcgtc
     2341 ggtcggtcca tcgcccgcaa tacgcggtcc gggacgttgg tgggtccggg aatctggagg
     2401 aagtgccggc cactgcgcgt ggcagacgcg gttttcgcga gcgactgcgt catgcgcggc
     2461 agcatacacc ccgggactcc ggctccggga agcgtcatcc cgcgtaatcg cccgtcatga
     2521 tccgggtagg gccaagtccc tactcctgac cgcaggaggg ttcatgcgga gcacaactcg
     2581 tcggacagtc acgttgatcg gcacggccgg gctcgcgctc gccgcgctcg ccatccattc
     2641 ttccccggtc tcgggcttca cccagagcgg ggtgaccatc aacttcatcg gcagcgggat
     2701 ccccatgggt cacgagtgga tcacccggat ggccggcatg gagctgctcg gcggcgaccc
     2761 ggtgatcaag accgatccca acgacccgcg caagcactgg acccagggcc tgcccaagga
     2821 tccggacatc aagaacgcgt ccgccgaggt caagcgcatc aaggaccaca aggtcaagga
     2881 cgcgcgctac gagtcgacct acgaggcggt gtacgccgcc atcatcggcg agcgctgggt
     2941 cgacatcgcc ggcttcaacg tcaccaagtc gcagatcggc agcggcccca actgcttcga
     3001 caacgtcgcc caggagccgg tcgacgtcca gtacgaccac ttcatgcggc gctacgacga
     3061 ccgcgatggc ggaggcggcg tcgacgcggc gcagcgctcg cgcgaccgct tcgtcgacta
     3121 cttcgtcacg gcggcgaccg cgccgcacgg cgtgatcacg gtgtgggacg gcggcggcta
     3181 cagcgcgctg gtcaccgtcg accgcaacta cttcctgttc ggccgcgcgg tccacctgtt
     3241 cgaggactcg ttcagctccg agcacacggt gcggctcgag agcgacatgt acgagaaggt
     3301 ccggcaggtc aagagctacc tgtgcgccgc cggctcggag cagcatacgc actcgcagaa
     3361 ggaggtgttc gactaccaga gcggcgacgt gatctggaag cccggcaccc ggttcgacgc
     3421 ggggtggagc ggctacaagg cgagcaacat gaagaccgtg gcgctggtcg gcaccgacgg
     3481 catgatcgat atgtgggcgg cgttcatccg cacgatgaac aaggacgaca aggaccgcgc
     3541 cgacgccgcg cgcaaggagg ccaaccgcct gatcgacagc tggctcaact tcgacaagga
     3601 cgaggtgaag aactggtaca acgacgcgtc gcaccgcgac gacacctacg tgctcggcaa
     3661 cggccagagc ggcaagggcc agacggtcaa gcagtgcatg gcgggcctca aggtcggcac
     3721 cgacgatcag gacaagtacg ccaagcagat cgaggacaac cggcgctact gcctctacaa
     3781 catcgtcccc gtgctcgggt tctcggacgc ccaggacccc gagctcaaga tgccctacaa
     3841 ctggcagtgg cgcgactcgc tgcactggca gaccccgccc aaggactgga agatccccga
     3901 cgtcaaggtg ccgcccgaga tcaaggtcac gatcaagagc gtcaagaaca aggactacat
     3961 ggtcgcgccc gacggcgtcg ccaaggacag ctggatctac tgcaagaacg gcgacgccct
     4021 gaagtgggtc gtggtcggca acaaggacag cgcgatcttc cggctcgaca gcgcgccgct
     4081 gttcctgagc tatcgactca ccaccggcgc ggtcaagctc tatgacaccg cgaccgacgc
     4141 cgagtacaag atcggcaagc aggacggcaa gcgcacgatc tacaacctgc gctacaagga
     4201 ctacatgtgg ctcgacgaca agtcgcccta cgtcagcagc aagggcgatc cgaagaagga
     4261 cagcg
//
LOCUS       DASVOR010001497         2281 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_365992,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001497 DASVOR010000000
VERSION     DASVOR010001497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365992"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF516_28315"
     CDS             complement(<1..458)
                     /locus_tag="VF516_28315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111681.1"
                     /translation="MELVLVCPGCRTRTADRLDLRTLEPRGDVLICACGRRYPVVDGV
                     PIVMADPSAYLRAEAATVVERDLSAEVVELLIAGGPDDAPYPRLVEHLSIYLDAHWGD
                     RAEPPPDGPGEPVTGGFGLRAVAARIAERAGHRVELAVELGCSTGRIVAEL"
     gene            652..>2281
                     /locus_tag="VF516_28320"
     CDS             652..>2281
                     /locus_tag="VF516_28320"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8111682.1"
                     /translation="MSKQPGFQPAAICNIVLLGHTGAGKTTLAEAILHRCGAVKRAGT
                     VGDATTVSDFEPEARAHHHSTSASLLFATSAGRQINLIDTPGAPELIGHALSALSAVE
                     TAVIVVNAAAGVESGTRRLFHAAGEAGLARMVVVNRIDENLAGLPALVEQLRAAFGPE
                     LHCINLPTGGGADVLDCFDHDAGAADFGSVADVHREMLESTIEIDDRDLERYLAGEAI
                     DVAELRKDFVEAMNRGHVVPILFTSATREVGVDDLLHILAAEAPSPVTGRPRRLCRGD
                     ALAEIPCDPARPLLAHVFKVTTDPYLGKIAMIRVLQGQLDGTAPFVMAGGRKALKAGH
                     LLKVEGREFVELTTAASAGDLVAIAKLDEIRIDAILRDPAAADDLAAARSRYPEPMYS
                     LAVALHHKADEVKLSTAMARLVEEDPTLRAAQDPQTHELVLSGLGELHLRITLEKLKN
                     RFGLDVTARTPTIAYRETITARAEGHYRLKKQTGGAGQFGEVFLRVEPLPRGGGYEFV
                     DDVFGGAIPSQYIPACDQGCQDAMERGVIAGYPVQ"
BASE COUNT          336 a          830 c          833 g          282 t
ORIGIN      
        1 agctcggcga cgatccggcc ggtgctgcag ccgagctcga ccgcgagctc gacccggtgg
       61 ccggcgcgct cggcgatccg ggccgcgacc gcgcgcagac cgaacccgcc ggtgaccggt
      121 tcccccggcc cgtcgggcgg cggctcggcg cggtcgcccc agtgcgcgtc gaggtagatc
      181 gacaggtgct cgaccagccg cgggtagggc gcgtcgtcgg ggccgcccgc gatcaggagc
      241 tcgacgacct cggcggacag gtcgcgctcg accaccgtcg cagcctcggc gcgcaggtag
      301 gccgacgggt cggccatcac gatcggcacg ccgtcgacca ccgggtagcg ccgcccgcac
      361 gcgcagatca gcacgtcgcc gcgcggctcg agcgttcgca ggtcgagccg atcggcggtg
      421 cgcgttcggc accctggaca caccaggacg agctccacgc cgcaacgata gcccacctgg
      481 cgaggggaga gcgcatacag ccgcgtccgc gatcgggatc cgcaccgatg aggagcgctg
      541 ccgggacgag cacccgtgag cggcgatcgg cttgcgctgc ggcgcaatcg ttgcggcgca
      601 atcgtggccc ggcgccctgc tgcacgggac cggcgggcgt agagtcgggc catgagcaag
      661 cagcccggct tccagcccgc ggcgatctgc aacatcgtcc tgctcggcca caccggcgcc
      721 ggcaagacga cgctcgcaga ggccatcctc caccgctgcg gcgcggtcaa gcgcgccggg
      781 acggtcggcg acgccaccac ggtgagcgac ttcgagcccg aggcgcgcgc ccaccaccac
      841 tcgaccagcg cgagcctgct gttcgcgacc agcgcggggc gccagatcaa cctgatcgac
      901 acgcccgggg cgccggagct gatcgggcac gcgctgtcgg cgctctccgc ggtcgagacc
      961 gcggtcatcg tggtcaacgc cgcggccggc gtcgagagcg gcacgcgccg gctgttccat
     1021 gccgcaggag aggccggcct ggcgcgcatg gtggtcgtca accggatcga cgagaacctc
     1081 gcggggctgc ccgcgctggt cgagcagctg cgcgcggcgt tcggccccga gttgcactgc
     1141 atcaacctgc cgaccggcgg gggcgccgat gtgctggact gcttcgacca cgacgccggg
     1201 gcggccgatt tcggctcggt cgccgacgtc caccgcgaga tgctcgagtc gacgatcgag
     1261 atcgatgacc gcgacctgga gcgctacctg gcgggcgagg cgatcgacgt cgccgagctg
     1321 cgcaaggact tcgtcgaggc gatgaaccgc ggccacgtgg tgccgatcct gttcaccagc
     1381 gcgacccgtg aagtcggggt cgacgatctg ctccacatcc tggccgcgga ggcgccgtcg
     1441 ccggtgaccg gccggccgcg ccggctgtgc cgcggcgacg cgctggccga gatcccgtgc
     1501 gatccggcgc gcccgctgct ggcgcacgtg ttcaaggtca cgacggatcc gtacctgggc
     1561 aagatcgcga tgatccgcgt cctgcagggc cagctcgacg gcactgcgcc gttcgtcatg
     1621 gccgggggac gcaaagcgct caaggccggc cacctgctca aggtcgaagg ccgcgagttc
     1681 gtcgagctga ccaccgcggc gtcggccggc gatctggtcg cgatcgccaa gctcgacgag
     1741 atccggatcg acgcgatcct gcgcgacccg gcggcggcgg acgacctcgc cgcggcgcgc
     1801 tcgcgctacc cggagccgat gtactcgctc gccgtcgcgc tccaccacaa ggccgacgag
     1861 gtcaagctgt cgaccgcgat ggcgcgcctg gtggaggagg acccgacgct gcgtgccgcg
     1921 caggacccgc agacccacga gctcgtgctg agcgggctcg gcgagctgca cctgcggatc
     1981 acgctcgaga agctcaagaa ccggttcggg ctcgacgtca ccgcgcgaac gccgaccatc
     2041 gcctaccgcg agaccatcac cgcgcgcgcc gagggccact accggctcaa gaagcagacc
     2101 ggcggtgcgg ggcagttcgg cgaggtgttc ctccgggtcg agccgctgcc gcgcggcggc
     2161 ggctacgagt tcgtcgacga cgtgttcggg ggcgcgatcc cgtcgcagta catcccggcg
     2221 tgcgatcagg gctgccagga cgcgatggag cgcggcgtga tcgccggcta cccggtccag
     2281 g
//
LOCUS       DASVOR010001498         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_161173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001498 DASVOR010000000
VERSION     DASVOR010001498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1995)
                     /locus_tag="VF516_28325"
     CDS             complement(<1..>1995)
                     /locus_tag="VF516_28325"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8111683.1"
                     /translation="RGPRPSPRADLAKWRAPLAALAARIAALRDRLRPLDASVGSNNW
                     VVSAAKSATGAAMVANDPHLSLQYPPLFHLAAMTSANAADNLNVTGGSFPGVPGALVG
                     RGAHVGWGVTVVGYDVTDVYMEQFLPQSNCSSAAPCVLFNGAPRATIPVPATFMVRTP
                     GGLVDAATLPAAALPVTIPPFLLVVPHHGPIVQPPDAGGHALSFRWTGHEGTTQDSKA
                     FYGLNTATDVDAAMTALNDYATGAQNFVLADDHGNIAYYPHALVPVRNFGDAAHLPWF
                     PLPGDGSAEWGDGTSACASPTAGQIASCWIANDQLPQGKNPAKGYFFTANADPTSLPG
                     NPEKGVSDDNNPLAHPPYMSFDWDDSSGFRATRIEQMMKDALAANGGKVSLTDMMKIQ
                     SDHVSRFGLAFAPIIAAIAAGAPSSSPPELGLAANMIAAWAASGADCPSGLTGVDPKA
                     SAADTTAAVVQSSSGCFLFHEFLRVLITNVFADDLALAGQGVSALNAVKAMLFMLSLD
                     PKDSATPALTTFCNDINPTTGQTIPPTKSCGAQVGAALVQAYITLRGLVGSDPNTWVW
                     GRVHTIQPVSLLALVTNGYSPGPYARPGGAFTVDVGNPSLTGGGINFSYGSGGNVRHI
                     SLMDPAKPVVKMQLPGPERDGPAITSRPDLLGQWVRNSYFD"
BASE COUNT          286 a          668 c          749 g          292 t
ORIGIN      
        1 gtcgaagtac gagttcctca cccactggcc gagcagatcg ggccggctgg tgatggccgg
       61 cccatcgcgc tccggcccgg gcagctgcat cttcaccacc ggcttggccg gatccatcag
      121 cgagatgtgg cggacgttgc cgcccgagcc gtaggagaag ttgatcccgc cgccggtgag
      181 cgacgggttg ccgacgtcga cggtgaacgc gccgcccggg cgcgcgtacg ggccgggcga
      241 gtagccgttg gtcaccagcg ccagcaggct caccggctgg atcgtgtgca cacggcccca
      301 gacccaggtg ttcggatcgc tgccgaccag cccgcgcagg gtgatgtagg cctgcaccag
      361 cgcggcgccg acctgggcgc cgcacgactt ggtggggggg atcgtctgac cggtcgtcgg
      421 gttgatgtcg ttgcagaacg tcgtcaacgc cggggtggcc gaatccttgg gatcgaggct
      481 gagcatgaac agcatggcct tgacggcgtt cagcgcgctg acgccctgac cggcgagcgc
      541 gagatcgtcg gcgaacacgt tggtgatcag cacgcgcagg aactcgtgga acaggaagca
      601 gccgctcgag ctctggacga cggccgccgt ggtgtcggcg gcgctcgcct tcggatcgac
      661 gccggtcagc cccgacgggc agtccgcgcc gctggcggcc cacgcggcga tcatgtttgc
      721 ggccagcccg agctcgggcg gcgaactgct cggagcgccc gcagcgatcg cagcgatgat
      781 cggagcgaac gccaggccga acctcgagac gtggtcggac tggatcttca tcatgtcggt
      841 gagcgacacc ttcccgccgt tcgcggcgag cgcgtccttc atcatctgct cgatccgcgt
      901 ggcgcggaac ccgctggagt cgtcccagtc gaagctcatg tacggcggat gggcgagcgg
      961 gttgttgtcg tcggacacgc ccttctccgg gttgccgggc agcgacgtcg gatccgcgtt
     1021 ggcggtgaag aagtagccct tggcggggtt cttgccctgc ggcagctgat cgttggcgat
     1081 ccagcacgac gcgatctggc ccgcggtcgg gctggcgcag gcgctcgtgc cgtcgcccca
     1141 ctcggccgat ccgtcgccgg gaagcgggaa ccacggcaga tgggcggcat cgccgaagtt
     1201 gcggaccggc accagcgcat gcggatagta ggcgatgttg ccgtggtcgt cggcgagcac
     1261 gaagttctgc gcgccggtgg cgtagtcgtt gagcgccgtc atcgcggcgt cgacgtcggt
     1321 cgcggtgttg aggccgtaga acgccttgct gtcctgggtg gtgccctcgt ggcccgtcca
     1381 ccggaagctc agcgcatgcc cgccggcgtc gggcggctgg acgatgggcc cgtggtgcgg
     1441 aacgaccagc aagaacggcg ggatggtcac cgggagcgcg gcggccggca gcgtcgcggc
     1501 atcgaccagc ccccccggcg tgcggaccat gaacgtcgcg ggcaccggga tcgtggcacg
     1561 cggcgcgccg ttgaacagca cgcacggcgc cgcgctcgaa cagttgctct gcggcaggaa
     1621 ctgctccatg tacacgtcgg tcacgtcgta gccgacgacg gtcacgcccc agccgacgtg
     1681 cgcgccgcgg ccgaccagcg cccccgggac gccggggaac gagccgccgg tcacgttgag
     1741 gttgtccgcc gcgttggccg acgtcatcgc cgcgaggtgg aacagcggcg ggtactgcag
     1801 cgacaggtgc gggtcattgg cgaccatcgc ggcgccggtg gcggacttcg ccgcggacac
     1861 cacccagttg ttggagccga cgctggcgtc gagcgggcgc agccgatcgc gcagcgcggc
     1921 gatccgggcg gccagcgcgg cgagcggcgc acgccacttc gcgaggtccg cccgcgggct
     1981 cggccgcggc ccgcg
//
LOCUS       DASVOR010001499         4039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_307738,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001499 DASVOR010000000
VERSION     DASVOR010001499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307738"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..949)
                     /locus_tag="VF516_28330"
     CDS             complement(<1..949)
                     /locus_tag="VF516_28330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase small subunit"
                     /protein_id="HEX8111684.1"
                     /translation="MPRRQFLQFCVGLTATLGLSRNAVGAVARALVEARRPSVIWLHF
                     QECTGCSESLLRAEHPTLEKLLLDIISLDYHETLMAAAGHQAEAARRAAMAANQGKYI
                     LVVEGAIPTGLDGNFCKIGGKTAVELIRESAADAASVIAIGSCASWGGMPSTAPNPTG
                     ASPVAAILGKPVLTIPGCPPNPYNFLTTVVHYLVLGKLPELDAKGRPTFAYGRLIHEN
                     CERRAHFDAGRFALEFGDEGHRQGWCLYKLGCKGPETYANCPTIQFGDAGVGSWPVGC
                     GHPCIGCTEQGVGFAKPIHELAKLLTVTPPTQFTRPGADQ"
     gene            complement(1363..3498)
                     /locus_tag="VF516_28335"
     CDS             complement(1363..3498)
                     /locus_tag="VF516_28335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004629 - phospholipase C activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C, phosphocholine-specific"
                     /protein_id="HEX8111685.1"
                     /translation="MTHRRDFLRAALSATAAGATLAFPPVIRRALAIPAHRRTGTIAD
                     VEHVVILMQENRSFDHYFGTLSGVRGFGDRFPIPVPDAPGIANRTVWFQSRGGAASGP
                     AAVAPFRLDTGASFALMRVTGTPHTWPSAQGAWGEGRLNQWPAFKTDRSMGYFTREDI
                     PFQFALAEAFTLCDAYHCSFHGGTNPNRLFLWTGTNDPRGAAGGPALTNEFDALDHDP
                     EGGYTWMTYTERLQAAGVSWQIYQNMADNFRDNPLAGFRRYRDAVAGRPGALAALRER
                     GLATRDLDLLADDVIHDRLPQVSWIIATEEGSEHPDSSSPAQGAAYTQRVLEALTANP
                     AVWSRTALLVNFDENDGFFDHVPPPAPPSTSADPAVLAGASTVDTAGEYHEVLPTGVD
                     PTKIFPLGKPYGLGPRVPMYVISPWSKGGWVDSQVFDHTSVLRFLERRFGVAEPSLSA
                     WRRAVCGDLTSAFDFADPDGRRFLDDLPDTTALAVAARALRDTTAPPTPVLPMLPVQE
                     EGTRPSRALPYELHVTARVRPGPERPQIELTFGNTGTAGAVFHVYDRKHLDRVPRRFT
                     VEPDRRLTDVWQLGGDGDYDLWLLGPGGFHRHLTGRAAPHAAAPEVDVAYVRGDLEIA
                     LHNRGVEPVELVLRANAYTSEAYRVVVPAGQDRVRRWPLERSAHWYDITVEARGLPGF
                     RRRFAGRVETGRHSLSDPAMGGPARGEPV"
BASE COUNT          598 a         1422 c         1453 g          566 t
ORIGIN      
        1 cctggtcggc gccggggcgc gtgaactggg tcggcggtgt cacggtcagc agcttggcca
       61 gctcatggat cggcttggcg aagccgacgc cctgctcggt gcaaccgatg cacggatggc
      121 cgcagccgac cggccatgac ccgacgcccg cgtcgccgaa ctggatcgtc gggcagttgg
      181 cgtaggtctc ggggcccttg cagccgagct tgtacaggca ccagccctgg cgatggccct
      241 cgtcgccgaa ctcgagcgcg aaccggccgg cgtcgaagtg cgcgcggcgc tcgcagttct
      301 cgtggatcag ccggccgtag gcgaacgtcg ggcggccctt ggcgtcgagc tcggggagct
      361 tgccgagcac gaggtagtgc acgaccgtgg tcaggaagtt gtacgggttg ggcggacagc
      421 cggggatggt caagaccggc ttgccgagga tcgcggcgac cgggctggcg ccggtcgggt
      481 tgggggccgt cgacggcatg ccgccccacg acgcgcacga gccgatcgcg atcaccgacg
      541 cggcgtccgc ggcggattcc cggatcagct cgaccgcggt ctttccgccg atcttgcaga
      601 agttgccgtc caggccggtc gggatcgcac cctccacgac gaggatgtac ttgccctggt
      661 tggccgccat cgccgcgcgc cgggcggctt cggcctggtg cccggccgcc gccatcaggg
      721 tctcgtgata gtcgagcgag atgatgtcca ggaggagctt ctcgagcgtc gggtgctcgg
      781 cgcgcaacag gctctcgctg cacccggtgc actcctggaa gtggagccag atcaccgagg
      841 gccgccgggc ctcgacgagc gccctggcga ccgcaccgac cgcgttgcgc gacaacccca
      901 gcgtggctgt cagaccgacg cagaactgga ggaactgccg gcgaggcatc ccgaagcgtt
      961 cctcggcgcc cggaaattca ttgtccggag ctgcgagtgg aagtttctcc atcatgaacg
     1021 ctagtccctc ctgatcgccg tgcgcggcag acgcgttgca agcgtggatt gcatcaagtg
     1081 cgccggctga ccgacgggat cgccggtgtc atttcccggc gatcgtgcaa aattggccgt
     1141 tgcttgccgg cagccctgca gcgtttgcga accgctgcca catcgacgtc ggtttgcgcg
     1201 cgtgacgctt cgcggcactg ccgatcacga ggacccgatg gagcacgcaa tctccgacga
     1261 gcccgagcag cggtgtcgga gcacgcccgc cccggaaccc gcgcgtccga gctcacgcgt
     1321 ggcagtccgg accgtgacga cggacgcgcg gtcgccgccg gctcacaccg gctcgccgcg
     1381 cgccggcccg cccatcgccg gatcgctcag cgagtggcga ccggtctcga cccggccggc
     1441 gaaccgccgc cggaagcccg gcagtccgcg ggcctcgacc gtgatgtcgt accagtgcgc
     1501 gctgcgctcg agtggccagc ggcgcacgcg atcctgcccg gccggcacga ccacgcggta
     1561 tgcctcgctg gtataggcat tggcccgcag caccagctcg accggctcga ccccgcggtt
     1621 gtgcagcgcg atctccaggt caccccgcac gtacgcgacg tcgacctcgg gggcggcggc
     1681 gtgcggcgct gcgcggccgg tgaggtggcg atgaaagccg ccgggcccga gcaaccatag
     1741 atcgtagtcg ccgtcgccgc cgagctgcca cacgtcggtc aggcgccggt cgggctcgac
     1801 cgtgaaccgc cgcggcaccc gatcgaggtg cttgcgatcg tagacgtgga acacggcgcc
     1861 ggcggtgccg gtgttgccga acgtcagctc gatctgcggg cgctcgggcc ccggccggac
     1921 ccgggccgtc acgtgcagct cgtagggcag cgcgcgcgac ggccgcgtcc cctcctcctg
     1981 caccggcagc atgggcagca ccggggtcgg cggcgcggtg gtgtcgcgca gggcgcgcgc
     2041 cgcgaccgcg agcgcggtgg tgtcgggcag gtcatcgagg aaccgccggc cgtcgggatc
     2101 cgcgaagtcg aacgccgagg tcagatcgcc gcacaccgcg cggcgccacg ccgagaggct
     2161 gggctcggcc acgccgaacc ggcgctcgag gaaccgcagc accgaggtgt gatcgaacac
     2221 ctgcgagtcg acccagccgc ccttgctcca cggcgagatc acgtacatcg gcacgcgcgg
     2281 ccccagcccg tacggcttgc cgagcgggaa gatcttcgtc ggatcgacgc cggtcggcag
     2341 cacctcgtgg tactcgcccg cggtgtccac cgtcgaggcg ccggccagca ccgcgggatc
     2401 ggccgacgtg ctcggcggag cgggcggagg cacgtggtcg aagaagccgt cgttctcgtc
     2461 gaagttcacc agcagcgccg tccggctcca caccgccggg ttcgcggtca gcgcctccag
     2521 cacgcgctgg gtgtacgccg cgccctgggc cgggctcgac gagtcggggt gctcggagcc
     2581 ctcctcggtc gcgatgatcc acgacacctg cggcagccga tcgtggatca cgtcgtcggc
     2641 gagcagatcg agatcgcgcg tggcgagccc gcgctcgcgc agcgccgcca gcgcgccggg
     2701 ccggccggcc accgcgtcgc ggtagcgccg gaaccccgcg agcgggttgt cgcggaagtt
     2761 gtcggccatg ttctggtaga tctgccagct gaccccggcg gcctgcagcc gctcggtgta
     2821 ggtcatccac gtgtaaccgc cctccggatc atgatcgagg gcgtcaaact cgttggtcaa
     2881 cgccgggccg cccgcggcgc cgcgcggatc gttggtcccg gtccacagga acaggcggtt
     2941 ggggttggtg ccaccgtgaa acgagcagtg gtaggcgtcg cacagcgtga acgcctcggc
     3001 gagcgcgaac tggaacggga tgtcctcgcg cgtgaagtac cccatcgagc gatcggtctt
     3061 gaacgccggc cactggttca gccggccctc gccccacgcc ccttgcgcgc tgggccaggt
     3121 gtgcggcgtg ccggtcaccc gcatcagcgc gaacgacgcc ccggtgtcga gccggaacgg
     3181 cgccaccgcc gccgggcccg acgcagcgcc gccacgcgac tggaaccaca ccgtgcgatt
     3241 cgcgatgccg ggtgcgtcgg gcaccgggat cgggaaccga tcgccgaacc cgcgcacacc
     3301 gctcaacgtg ccgaagtagt gatcgaacga gcggttctcc tgcatcagga tcacgacgtg
     3361 ctcgacatcg gcgatcgtgc cggtcctgcg atgcgccggg atcgccagcg cgcggcggat
     3421 caccgggggg aacgccagcg tggcccccgc cgcggtcgcc gacagcgccg cgcgcaggaa
     3481 gtcgcgtcgg tgggtcatgc gccggtcttc gcacctcacg atgacggcgc gatggcggcc
     3541 gatcgccatt gccgagacgg gatcgcgaca ggcgatctgg tctcggagtc aggtcgcaag
     3601 tccaccaggt cattgtcagg cctgacagag ccgctcagcc gcggcggctc gcgctcgatc
     3661 ggcctggcga gagcggacca gtggcaaatg ccggcaccca cgtgggcgcg taccatcggc
     3721 acatctggga caacgcatcg atggccgacg agcgcggggg aaggccagcg gccgtgaacc
     3781 agggcacctg gctggctgtg caggacgcgt gaccgcgccg tgactcgcgt ggacagccgc
     3841 gcccgacggt cgaggccgac atggcgcgcc ctgccgcaca cgactcaccg cgctgacgcc
     3901 ggcatgtgcg ctccgcgacc gggtttattc tcgatctggt ttcggagtca ggttgcaagt
     3961 ccaccaggtc attgtcaggc ctgatagagc cgctcagccg cggcggctcg cgctcgatcg
     4021 gcctggcgag agcggacca
//
LOCUS       DASVOR010001500         2487 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_483025,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001500 DASVOR010000000
VERSION     DASVOR010001500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483025"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..726)
                     /locus_tag="VF516_28340"
     CDS             complement(94..726)
                     /locus_tag="VF516_28340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111686.1"
                     /translation="MDLFEELQRIIAPFPAEQIDAALADRPSTFDVDARWYTSHEGPP
                     RTTSDRSPFHGDALLAGMQAVCLLGSYLPAGDPRRLGVPRLHAKILARLANPELLLFG
                     GTRELKIEGKPYVAPGYQSNPSQHGVDSGALVVAHGYVYFRPGRIPPTDTENLSRARD
                     AAELHYPQQVFPWSGLYHVWQAALSPGVAALVARAADTPLPAGGKPVSET"
     gene            complement(739..1230)
                     /locus_tag="VF516_28345"
     CDS             complement(739..1230)
                     /locus_tag="VF516_28345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111687.1"
                     /translation="MSAGVGADVEALCSKCGDVWHVVVAKVGDQIVKVQCKQCSGYHR
                     YKSPHGAPAEKKLPAATRPVREPKPPVERFEKPAVTADLTRPTRSYRASELFEVGERL
                     DHPSFGQGVVELAEPGKITVFFASGRRVLVQAKAAASAGLARPKPFDHTNPPAGGKPV
                     SET"
     gene            1336..2253
                     /locus_tag="VF516_28350"
     CDS             1336..2253
                     /locus_tag="VF516_28350"
                     /inference="COORDINATES: protein motif:HMM:NF016932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEX8111688.1"
                     /translation="MSQASADALVASGNELLRVENYSDAAAKFERAAQIFPPHALAWK
                     GLGHALLCMGRAQEAARAFDRAIGLRPESATALWGGAVAHAEIGNKVVAQNYLRRTLA
                     LQPTWIEMARSVSHLVPLLQVSTRVADAMRAALGTFSTRSYRHVSDENQSIEVARFAE
                     QPSFGRWTYATLGLANTVWRELGRLRVELLFATTVDTEICGQVLATLAFHLSNTGFFP
                     EPGVMVRDVIGALGAGDLSQRLPHVYVTVPRLWRVSLPIVAGPPPVTLAQVVAVSEAE
                     YTAWRADVAGFEKLLAARNVDLTDLRRPG"
     gene            2284..>2487
                     /locus_tag="VF516_28355"
     CDS             2284..>2487
                     /locus_tag="VF516_28355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111689.1"
                     /translation="MSQIAGTTRLRCPAGGRRLPVRGPACHNHAMKRLGSAALISLLA
                     SLSMVACENGSRLDSSVKNAPQKD"
BASE COUNT          325 a          858 c          941 g          363 t
ORIGIN      
        1 cgggcggtcg gccagcgcgg cgtcgatctg ctcggcgggg aacggcgcga tgatccgctg
       61 gagctcctcg aagaggtcca tcgccgcgcc gccctaggtc tcgctgaccg gcttgccgcc
      121 ggcgggcagc ggcgtgtcgg cggcgcgggc gaccagcgcg gcgacgccgg gcgacagcgc
      181 ggcctgccag acgtggtaga gccccgacca cgggaacacc tgctgggggt agtggagctc
      241 ggcggcgtcg cgcgcgcgag agaggttctc ggtgtcggtc ggcgggatcc ggccgggccg
      301 gaagtacacg tagccgtgcg cgacgacgag cgcgccgctg tcgacgccgt gctgcgacgg
      361 gttggactgg tagcccgggg cgacgtaggg cttgccctcg atcttgagct cgcgcgtgcc
      421 gccgaacagc agcagctcgg ggttggcgag ccgggcgagg atcttggcgt gcagccgcgg
      481 cacgccgagc ctgcggggat cgccggccgg gaggtagctg ccgagcaggc agaccgcctg
      541 catcccggcg agcagggcgt cgccgtggaa cggcgacctg tccgatgtgg tcctgggcgg
      601 gccctcgtgg ctcgtgtacc accgggcatc gacgtcgaag gtcgacgggc ggtcggccag
      661 cgcggcgtcg atctgctcgg cggggaacgg cgcgatgatc cgctggagct cctcgaagag
      721 gtccatcgcc gcgccgccct aggtctcgct gaccggcttg ccgccggcgg gcgggttggt
      781 gtggtcgaac ggcttggggc gggccagccc ggcggacgcc gcggccttgg cctggaccag
      841 gacgcgccgg ccggacgcga agaacacggt gatcttgccg ggctcggcga gctcgaccac
      901 gccttgcccg aagctcgggt ggtcgagccg ctccccgacc tcgaacagct cgctggcgcg
      961 gtagctgcgg gtcggccggg tcagatcggc ggtgaccgcc ggcttctcga accgctcgac
     1021 cggcggcttg ggctcgcgca ccggccgggt ggcggcgggc agcttcttct cggccggggc
     1081 gccgtgcggc gacttgtagc ggtggtagcc gctgcactgc ttgcactgaa cttttacgat
     1141 ctggtcgccg accttggcga cgacgacgtg ccagacgtcg ccgcacttcg agcacagcgc
     1201 ttcgacgtcg gcaccaactc ctgcggacat ggactggcga gcatagcagc tgtgccggca
     1261 cagaagggca ggacgccgac tctgctggcg atccggcgca gggaccccgc ggcttttgtt
     1321 aagatcccct ctaccatgag ccaggcctcg gccgatgcac tcgtcgcctc cgggaacgag
     1381 ctgctacgcg tcgagaacta ttcggacgcg gcggccaagt tcgagcgcgc cgcccagatc
     1441 tttccgcctc acgcactggc ctggaagggg ctgggccacg ccctcttgtg catgggacgc
     1501 gcccaggagg ccgcgcgggc gttcgaccgt gcgatcgggc ttcgccccga gagcgcgacg
     1561 gcgctgtggg ggggcgcggt cgcgcatgcc gagatcggca acaaggtcgt cgcccagaac
     1621 tacctgcgcc gcaccctcgc gctgcagccg acctggatcg agatggcgcg ctcggtcagc
     1681 cacctggtgc cgctgctcca ggtctcgacc cgggtcgccg acgcgatgcg cgccgcgctc
     1741 ggcacgttct cgacccgcag ctaccgccac gtcagcgacg agaaccagtc gatcgaggtc
     1801 gcgcggttcg ccgagcagcc gagcttcggg cggtggacct acgccacgct cgggctcgcc
     1861 aacacggtgt ggcgcgagct cggccggctg cgggtcgagc tcctgttcgc gaccaccgtc
     1921 gacaccgaga tctgcggcca ggtgctggcg acgctggcgt tccacctgtc gaacaccggg
     1981 ttcttcccgg agccgggcgt gatggtgcgc gacgtgatcg gcgcgctcgg cgcgggcgac
     2041 ctgtcgcagc ggctgcccca cgtgtacgtc acggtgccgc ggctgtggcg ggtgtcgctg
     2101 ccgatcgttg ccgggccgcc gccggtgacg ctcgcccagg tcgtcgcggt cagcgaggcc
     2161 gagtacaccg cgtggcgggc cgacgtcgcc ggcttcgaga agctgctggc cgcgcgcaac
     2221 gtcgatctga ccgacctgcg gcgtcccggg tgacgtgagg cgagcctgcg cggcgcctgc
     2281 cgcgtgagcc agatcgcggg aaccacacgg ctgcgctgcc cggccggcgg ccgacgcttg
     2341 ccagtccgcg ggccggcgtg ccacaaccac gccatgaagc gtctcgggtc ggccgcgttg
     2401 atctcgttgt tggcatcgct gtccatggtc gcgtgcgaga acggctcgcg gctcgacagc
     2461 tcggtgaaga acgccccgca gaaggac
//
LOCUS       DASVOR010001501         3691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_366028,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001501 DASVOR010000000
VERSION     DASVOR010001501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366028"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1118
                     /locus_tag="VF516_28360"
     CDS             270..1118
                     /locus_tag="VF516_28360"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - acyl-[acyl-carrier-protein]
                     desaturase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HEX8111690.1"
                     /translation="MSDIAQHVAISHKVEFEDLDWEQARKVGLTRQEIESLTYFADIE
                     SQTVYYFLEVAKLQVARDPELLTFLTLWNYEEYFHSYALTRLLAECGVPVESATERST
                     KIRAGARFKARLEDLVQSLIARLMPRTFVALWMFWGSLQECLTTQAYEELARNTQNPV
                     LAELCRRIAKQERRHFAYYYGQARRRLAGQTWAQRFTRWIAQRFYAPVGGGVKTDAEG
                     ARLVAELFPGDRIFEVMRYIEKRMGQLPGMDGLDCCTRWAARVQPMLPPETRASSIPL
                     ELSAAA"
     gene            complement(1122..1535)
                     /locus_tag="VF516_28365"
     CDS             complement(1122..1535)
                     /locus_tag="VF516_28365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111691.1"
                     /translation="MASAEPNKPTGDRPIHAPDVPQLAAQIDGKFAQETVDPAWSAGA
                     EYQLRQALERTPSPGTTLASISCRRSLCRVESLHTSLEAFQQYVESSYLADGRPLWNG
                     AFTSLVTEQSDTQVKAVSFVAKEGSELPAAEPVGE"
     gene            complement(1793..2254)
                     /locus_tag="VF516_28370"
     CDS             complement(1793..2254)
                     /locus_tag="VF516_28370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111692.1"
                     /translation="MNKRMLSMITALTVAAATFGTAGADTVPANMGKATVGAQTGLFN
                     YNTMTGAVTAFGFAGLRSWQVGLPLDIFGPKLVTVTSHTTNPLFGPTWRIVTSNAVGT
                     GFGASATAPIPLSPLFLPITRGIPFVVPGGVCSVVASMNAGSSIAVLNYNM"
     gene            complement(2518..2859)
                     /locus_tag="VF516_28375"
     CDS             complement(2518..2859)
                     /locus_tag="VF516_28375"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8111693.1"
                     /translation="MAIFSFGDFEADEDRFELRRRGSGVRVQRLVLETIFHFLRSGGK
                     LITKRELARATRPGARVSDAAVTRAIMLARRALMDHAGTVIVTVHGVGYRFDSDVREV
                     EIPDGSRGSDN"
BASE COUNT          579 a         1220 c         1262 g          630 t
ORIGIN      
        1 gcggccggga tgcgtggcct gttgcacatc gcacggttgc ggcgcggtga cctggagggc
       61 ggcgccggcg gggtggcgcg cgcagcggcc gcgcgcgacg tgctgcacga cctcggcgcg
      121 gtcgacgccg accgcgtcgc ggcgcacatg ctgccctggc cggcgggaaa ctgaggtacg
      181 ctggctcgca atcgagcggg tgatccgatg caggccatga gccgtacctg caccggacgg
      241 cgtttccgcc gagatgaggg taaccagcag tgtccgatat cgctcagcac gtcgctatct
      301 cgcacaaggt ggagttcgag gatcttgact gggagcaggc gcgcaaggtc gggttgaccc
      361 ggcaggagat cgagagcctg acctacttcg ccgacatcga gagccagacc gtctattact
      421 tcctggaggt cgccaagctc caggttgcgc gcgaccccga gctgctcacg ttcctgaccc
      481 tgtggaacta cgaggagtac ttccactcgt acgcgctgac ccgcctgctc gccgagtgcg
      541 gcgtgccggt cgagagcgcg accgagcgct cgacgaagat ccgcgcgggc gcccggttca
      601 aggccaggct cgaggacctg gtccagagcc tgatcgccag gctcatgccg cgcacgttcg
      661 tcgcgctgtg gatgttctgg ggctcgctcc aggagtgcct gaccacgcag gcctacgagg
      721 agctggcgcg caacacccag aacccggtgc tcgccgagct gtgccgccgg atcgccaagc
      781 aggagcgccg ccacttcgcg tactactacg gccaggcccg gcgccggctc gccggccaga
      841 cctgggccca gcggttcacg cgctggatcg cgcagcggtt ctacgcgccg gtcggcggcg
      901 gggtgaagac cgacgccgag ggcgcgcggc tggtcgccga gctgttcccc ggcgaccgga
      961 tcttcgaggt catgcgctac atcgagaagc ggatgggcca gctgcccggc atggacgggc
     1021 tcgactgctg cacgcggtgg gcggccaggg tccagccgat gctgccgccc gagacccgcg
     1081 cgtcgtcgat cccgctcgag ctgtcggcgg cggcctgagg cctactcccc gacgggttct
     1141 gccgccggga gctcgcttcc ttccttggca acgaagctca ccgctttcac ctgagtgtcc
     1201 gattgttcgg tcaccagcga cgtgaaagcg ccgttccaca gcggccggcc atccgccagg
     1261 tacgacgact cgacgtactg ctggaaggct tccaggctgg tgtggagcga ctccactcga
     1321 caaaggcttc ggcgacagct gatgcttgcc agcgtggtcc ccggagacgg cgtgcgctcc
     1381 agggcctgcc tgagctggta ctcggcaccg gcgctccagg ccggatcgac ggtttcctgg
     1441 gcgaacttgc cgtcgatctg ggccgccagc tgggggacat cgggcgcatg gatcgggcga
     1501 tcgccggtgg gcttgttcgg ttcggcgctc gccacgaacg acggccgagg aggtggagtg
     1561 tcctccaccc cccggcgccg cgcgctactt gccgcctgct cgccgatcgg cgtctgccgc
     1621 gacgccgagc tcgcactcgt gtcccgaagt ggctgatccg gtgaatgtac cgtgccgtgc
     1681 cacacggcga gagcgagtcc ggcgatcagg gtcgtaggaa cgatccaggc cagggttcga
     1741 tacctgctca taggtctcct gtatcgatgc ggcggagcag acggcggggg gctcacatgt
     1801 tgtaattgag gacagcgatg ctcgacccgg cgttcatgct ggcgaccacg gagcacacac
     1861 cgcccggcac gacgaagggg ataccgcgcg tgatcggcag gaaaaggggc gagagcggga
     1921 tgggggcggt ggcgctcgca ccaaacccgg tgccgaccgc attgctggtc acgatgcgcc
     1981 acgtcgggcc aaacaggggg ttcgtggtgt gtgacgtgac cgtcacgagc ttcggtccga
     2041 aaatgtccag gggcaatcca acttgccagc tgcgaaggcc ggcgaagccg aacgccgtca
     2101 cggctccggt catggtgttg tagttgaaca ggcccgtctg ggcgcccacc gtggccttcc
     2161 ccatgttcgc cggaacggtg tccgcgccag cggtaccaaa ggttgcggcg gcgacagtca
     2221 gtgcagtgat catcgaaagc attctcttgt tcatgacatc ctccatcatt ggcggccatc
     2281 agcgaccgcc ggtcatgagt agcgcaggcg tcgtgattga tttgtgagct attcttgatt
     2341 tctccggttg cgccagacga aagacagcgc ctcttgcacg agggtaagcg tgcttggaga
     2401 aatggccgtg taccgtcggt agcgaacctc acgcgccggc gcgctgggtg cgagagaccc
     2461 gcaagtccgg atctgtggca gccccgcagt gaatcccacc acccggctga cgctcggtca
     2521 gttgtcgctg ccccgagagc catccgggat ctccacctct cgaacatcgg agtcgaagcg
     2581 atagccaacg ccatgaacgg tgacgatcac tgtgccggca tgatccatga gcgctcttct
     2641 ggcgagcatg atcgctcggg tcaccgccgc atcgctgacc ctggcgccag ggcgcgtggc
     2701 ccgggccagt tcgcgcttcg tgatcagctt gcccccgctt cgcaagaaat ggaagatggt
     2761 ctcgagtacc aggcgctgca cgcgcacgcc gctcccgcga cggcgcagct cgaacctgtc
     2821 ttcgtcggcc tcgaagtccc caaaactgaa gatcgccatt ccctccctcc ttcaccatca
     2881 cgcggtcaac catcgaaggt tgtagctgtc atgcctgtcg cctgcatagg catcgcgagc
     2941 ctcggctcgc cgcggtcctt cgccagccgg ccggcgaacg tcgcggtcgg ggtgcggcgc
     3001 tggaccgcgc ggtcagcctc tgcgcgaggt gatcgatgcg atcgtcgagg acgtgggaga
     3061 cctggtggtc gctacccctg tagcccgccg cctgcagttc ggcctcgatc agccgggtga
     3121 gctccctcgt gtcgtaccac cgaagccgtt ggttcaccgg cgctcgcgct gccttgcgcc
     3181 tggccgcgat ggccagaacc ttcttgttgt tgatgtcgtc ccgcaccggc ttgtcgcacc
     3241 gccatggtcg cgacgctgtg cacgtttcgt gcttggagtg gtagcccagg agcgcgacga
     3301 actcggcaat attccgatgc tgttgttgtg ccgacgcatt gcagggacca tccagactgt
     3361 ggtggatcgg ctcggagggc tctaccgaca cagaactcgc atcgcgccca ccacagccca
     3421 caaggtgagc tgctcctggt ttgctgtcca gcagcggtgc gcgtaccggc tcagcggttc
     3481 acgccggggc ggatccggcc gcggtcgaga tcgcgcgcgt cagcgcagca cggtcagcgt
     3541 tcgcatcgtg ccgcgggtga cgccgaactg ctcgtggacg gcggcgcgcc ggaccgcctc
     3601 gcggggcgcg atctcgtcgg cgacgagctg ggcgtaggcg gtgtagacgc gggcggtctc
     3661 ctcggcggac tcgcgcacga gctcgacccg g
//
LOCUS       DASVOR010001502         2308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_541398,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001502 DASVOR010000000
VERSION     DASVOR010001502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2308
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541398"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="VF516_28380"
     CDS             <1..1906
                     /locus_tag="VF516_28380"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111694.1"
                     /translation="AYVREAHVRYLRWGAEGKAQQLRRAYPELELQPTGAAAAALRPE
                     QLDRLSVIKALQTISSVMDQDVLSRTLLRFVLEEGGARRVVLITSRNGELEIAAAASV
                     DEPCPPLDGARVPRSVLSYVLWTQDSVVFDAADDAGRFANDPYFAGARPRSVLCMPVR
                     LRADSVALLYLENDLVPGTFTPERLLALELLAAQAAISLENARLLERERTGRIEAEAA
                     EHRGRLLGEATALLSQTLESRGVLDALARLFTRSFADCVVIDLEDSGALVRTAGAHRD
                     PDNEPILRELAARYAAQPRSPMWQVQHTGQVLEIPILTDDQLRSYCVDDHHAELIRRL
                     GARSAVYVPLCARDAVIGVLSLVATTPNRFARADVELAVDLGRRMALAIDNARLLDET
                     RRALHLRDEFLRIASHELRTPLASLRLSAQGLLRAAERNRAVSPEILDRTLHRVLGNT
                     IRLEQLTSELLDVTRIEQGRLQLNPIEVALDAIVREAVEHIEADLAAAGSSVSIECTA
                     PVVGRWDPSRLDQVVTNLLSNAAKFGAGKPIEIRIDQLGATARLTVTDHGIGIDPARR
                     RHVFDRFERAVPSSSYGGLGLGLYIARSIVVAHGGTITVDSELGAGSTFTVTLPRAAP
                     GPSTERAAAN"
     gene            complement(1918..>2308)
                     /locus_tag="VF516_28385"
     CDS             complement(1918..>2308)
                     /locus_tag="VF516_28385"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEX8111695.1"
                     /translation="VAPAAPAASSAAPAPRVPEKIVQLNAPGAAVDVAAALPAGYVTV
                     VDFWSQSCEACGPLGARLETGVADEPRVVIRKVDVGDGDTPVAAAYQIAALPHFFIYD
                     KHKRLRYDLVGPDCLQAARYARQLADE"
BASE COUNT          295 a          849 c          851 g          313 t
ORIGIN      
        1 cgcctacgtg cgcgaggccc acgtccgcta cctccggtgg ggcgccgagg gcaaggcgca
       61 gcagctgcgc cgcgcctacc ccgagctgga gctccagccg accggcgcgg cggccgcggc
      121 gctgcgcccc gagcagctcg accggctgtc ggtgatcaag gcgttgcaga ccatctccag
      181 cgtcatggac caggacgtcc tgtcccgcac gctgctgcgg ttcgtgctcg aggagggcgg
      241 cgcgcggcgc gtggtcctga tcacctcgcg caacggcgag ctcgagatcg ccgccgcggc
      301 gagcgtcgac gagccgtgcc cgccgctgga cggcgcgcgc gtcccgcgct cggtgctgtc
      361 gtacgtgctg tggacccagg actccgtggt gttcgacgcc gccgacgatg ccggccggtt
      421 cgcgaacgat ccgtacttcg ccggcgcccg accgcgctcg gtgctgtgca tgccggtccg
      481 cctgcgggcc gattcggtcg ccctgctcta cctcgagaac gatctggtcc cgggcacgtt
      541 caccccggag cggctgctcg cgctcgagct gctggcggcg caggcggcga tctcgctcga
      601 gaacgcccgg ctgctcgagc gcgagcgcac cgggcggatc gaggccgagg cggccgagca
      661 ccgcgggcgg ctgctcggcg aggcgaccgc gctcctgtcc cagacgctcg agtcgcgcgg
      721 tgtgctcgac gcgctggccc ggctgttcac gcgatcgttc gcggactgtg tggtgatcga
      781 tctcgaggac agcggggcgc tggtgcggac cgccggcgcg caccgggatc cggacaacga
      841 gccgatcctc cgcgagctgg cggcgcgcta cgcggcgcaa ccgaggtccc cgatgtggca
      901 ggtccagcac accggccagg tgctcgagat ccccatcctg accgacgatc agctccggtc
      961 gtactgcgtc gatgatcacc acgccgagct gatccgccgg ctcggcgcgc ggagcgcggt
     1021 gtacgtgccg ctgtgcgccc gggatgcggt gatcggcgtg ctgtcgctgg tcgcgacgac
     1081 cccgaaccgg ttcgcccgcg ccgacgtcga gctcgcggtc gatctcgggc gccggatggc
     1141 gctggcgatc gacaacgcgc ggctgctcga cgagacccgg cgggcgctgc acctgcgcga
     1201 cgagttcctg cggatcgcgt cgcacgagct ccgcaccccg ctcgcgtcgc tccggctcag
     1261 cgcccagggg ctgctgcgcg ccgccgagcg aaatcgcgcg gtgtcgccgg agatcctgga
     1321 ccgcaccctg caccgcgtgc tcggcaacac gatccgtctc gagcagctca cgtcggagct
     1381 gctcgacgtc acccgcatcg agcagggccg cctccagctc aacccgatcg aggtcgcgct
     1441 cgacgcgatc gtccgcgagg cggtggagca catcgaggcc gatctcgccg ccgcgggatc
     1501 ctcggtgtcg atcgagtgca cggcgccggt cgtgggacga tgggatccgt cgcggctcga
     1561 tcaggtggtc acgaacctcc tgagcaacgc ggccaagttc ggcgccggca agccgatcga
     1621 gatccggatc gatcagctcg gcgccaccgc gcggctgacc gtgaccgacc acggcatcgg
     1681 catcgacccg gcgcgccgcc gccacgtgtt cgatcgcttc gagcgcgcgg tcccgtcgtc
     1741 gagctacggc gggctcgggc tggggctgta catcgcccgg tcgatcgtcg tcgcccacgg
     1801 cggcacgatc accgtcgaca gcgagctcgg cgccggctcc acgttcaccg tgaccctgcc
     1861 gcgcgccgcg cccgggccga gcaccgagcg ggcggccgcg aactgacgcg cggccggcta
     1921 ctcgtcggcg agctggcggg cgtagcgcgc ggcctggagg cagtcggggc cgaccaggtc
     1981 gtagcgcagc cgcttatgct tgtcgtagat gaagaagtgg ggcagcgcgg cgatctggta
     2041 ggccgccgcg accggggtgt cgccgtcgcc gacgtcgacc ttgcggatca ccacgcgcgg
     2101 ctcgtcggcg acgccggtct ccagccgcgc gccgagcggg ccgcacgcct cgcacgactg
     2161 gctccagaag tcgaccacgg tgacgtagcc ggcgggcagc gccgcggcga cgtcgaccgc
     2221 cgcgccgggc gcgttgagct ggacgatctt ctccgggacg cgcggtgccg gggcagccga
     2281 cgaggcggcg ggcgcggccg gagcgacc
//
LOCUS       DASVOR010001503         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_512051,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001503 DASVOR010000000
VERSION     DASVOR010001503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512051"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..237)
                     /locus_tag="VF516_28390"
     CDS             complement(<1..237)
                     /locus_tag="VF516_28390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8111696.1"
                     /translation="MPARYLIGIDLGTTNSAVACVDTRAADPRVRVFEVAQLVAPGEV
                     ATRRQLPSFVYLAGDVDLAPHETALPWRPAASSAA"
     gene            complement(257..727)
                     /locus_tag="VF516_28395"
     CDS             complement(257..727)
                     /locus_tag="VF516_28395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111697.1"
                     /translation="MAMTPAYALAHVFLPNLLKLKGHATVVSAIERRDLVYFDPLWAQ
                     AHIKHNPHVTSETRDPYRIATMSLPAPTEMGEAHMAGIVIKTNDPSFMRYFTLEHDFV
                     LAKQANRTLLCEREGQKHTKIGDGPVLTGNAGDDARAFVNCFMELMIPTKVTRK"
     gene            complement(859..>1976)
                     /locus_tag="VF516_28400"
     CDS             complement(859..>1976)
                     /locus_tag="VF516_28400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8111698.1"
                     /translation="EIADDGGNLVLERIAVGDHILLGGDNMDLALAAMVGRGIGRPLD
                     ALPQRALVHTCRRAKERLLGSVSPVGSVGSVGDGAPDKLPIAILGRGSKLIGGTIKAE
                     VTRAQAEAVLVDDFFPEVAGDARAEQRRAGGLRELGLPYAHDPAITRHLAEFVARFGR
                     TPSHVLFNGGVMKAPRLVARVVERLRAWAGRDVRVLDGADPDLAVALGAAHYGQVRRG
                     KGIRIRGGTARAYYIGIESAMPAIPGFAPPVKALCVAPQGMEEGSTAELPDDELGLVV
                     GETSVFRFFAAGARPDIPELVTDQAGALIDADTEGVVELDPVEKLVAADADHPAGQLV
                     PVRLESHVTEVGTLELWCVARDGRGRWKLEYSVRERS"
BASE COUNT          273 a          701 c          716 g          286 t
ORIGIN      
        1 ggccgcggac gatgcggccg ggcgccacgg cagcgcggtc tcgtggggcg cgaggtcgac
       61 gtcgccggcg aggtaaacga aggatggcag ctggcggcgc gtcgcgacct cgccgggggc
      121 gaccagctgc gcgacctcga acacccgcac cctcggatcg gcggcgcggg tgtcgacgca
      181 ggccaccgcc gagttggtgg ttccgaggtc gatgccgatc aggtaccgcg cgggcacaag
      241 cccatgtacc gcggttctac ttcctcgtca ccttcgtcgg gatcatcagc tccatgaagc
      301 agttgacgaa cgcccgggcg tcgtccccgg cgttgccggt cagcaccggg ccgtcgccga
      361 tcttggtgtg cttctgaccc tcgcgctcgc acagcagggt gcggttggcc tgcttggcga
      421 gcacgaagtc gtgctcgagc gtgaagtacc gcatgaacga cgggtcgttg gtcttgatca
      481 cgatcccggc catgtgcgcc tcgcccatct cggtcggcgc cggcaggctc atggtcgcga
      541 tgcggtacgg gtcgcgggtc tccgaggtga cgtgcgggtt gtgcttgata tgggcctggg
      601 cccacagcgg atcgaagtag accagatcgc gccgctcgat cgcggacacg acggtggcgt
      661 ggcctttgag cttgagcagg ttgggcagga acacgtgcgc gagcgcgtac gccggagtca
      721 tcgccatgcc caacatatac gcacgaggca catggcctcg ccgtgccatc catggccgca
      781 tcggcgccgc gtattgcggc acgttgtgaa ccgcggcatg cgtcggcctg cgacgcgcgt
      841 cgatgctcac ccaccgtgtc agcttcgctc gcgcaccgag tactccagct tccagcggcc
      901 gcggccatcg cgtgcgacgc accatagctc cagggtgccg acctcggtga cgtgggactc
      961 cagccggacc gggacgagct ggccggcggg gtggtcggcg tcggcggcga ccagcttctc
     1021 gaccgggtcg agctcgacca cgccctcggt gtcggcgtcg atcagcgcgc cggcctggtc
     1081 ggtcaccagc tcggggatgt cggggcgcgc cccggcggcg aagaaccgga acaccgaggt
     1141 ctcgccgacc accaggccga gctcgtcgtc gggcagctcg gcggtcgagc cctcctccat
     1201 gccctgcggc gcgacgcaca gcgccttgac cggcggcgcg aaccccggga tcgcgggcat
     1261 cgcgctctcg atgccgatgt agtacgcgcg cgcggtgccg ccgcggatcc ggatgccctt
     1321 gccgcggcgg acctggccgt agtgcgccgc gcccagcgcg accgcgagat cggggtcggc
     1381 accatcgagt acgcggacgt cccggccggc ccacgcgcgc agccgctcga ccacccgggc
     1441 gacgagcctg ggcgccttca tcacgccgcc gttgaacagc acgtggctcg gcgtccggcc
     1501 gaaccgggcc acgaactcgg cgaggtggcg ggtgatcgcc ggatcgtggg cgtagggcag
     1561 gccgagctcg cgcaggccgc cggcccggcg ctgctcggcg cgcgcgtcgc cggcgacctc
     1621 ggggaagaag tcgtcgacca gcaccgcctc ggcctgggcc cgggtgacct cggccttgat
     1681 cgtgccgccg atcagcttgc tgccgcggcc gaggatcgcg atcggcagct tgtccggcgc
     1741 gccgtcaccg accgaaccca ccgaacctac cggactcacc gaacccagta gtcgctcctt
     1801 ggcgcgccgg caggtgtgga ccagcgcgcg ctgcggcagg gcgtcgagcg gccggccgat
     1861 gccgcggccg accatcgccg cgagcgcgag gtccatgttg tcgccgccga gcaggatgtg
     1921 atcgccgacc gcgatccgct cgagcacgag gttgccgccg tcgtcggcga tctcga
//
LOCUS       DASVOR010001504         3200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_44830,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001504 DASVOR010000000
VERSION     DASVOR010001504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44830"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..817)
                     /locus_tag="VF516_28405"
     CDS             complement(185..817)
                     /locus_tag="VF516_28405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8111699.1"
                     /translation="MKPLVVLLHGLARGPGSMARLGAHLAAHGFDTRSHSYPSRRASI
                     AEIARALTDTIAREAAGRPISAVTHSLGGVVARHLHDPRIAWQRIVMLAPPNQGSRLA
                     RDLAINPLFRWFYGPAGRELADASGWPAPPAPFAVIAGTRARALINPTSWTAGRRFPP
                     GVPNDGTVAVAETRLDGMADFAEVDATHTWIMNDPCVHGLVVRYLRHGSF"
     gene            complement(814..2256)
                     /locus_tag="VF516_28410"
     CDS             complement(814..2256)
                     /locus_tag="VF516_28410"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8111700.1"
                     /translation="MVYRILAWFLRVVTRVFFRQVEIVGVENIPPTGGVLFAGNHPNS
                     LIDPILIITSCGRKVHFAAKDTLFKGRLMRAVLRGLGAVPIRRRDDRDGTPPRDAPAQ
                     PVSAPALDNESAFAAMFAVLESGGAIGIFPEGLSHDDSQLARLKTGAARLALGGAHRA
                     TAPISIVPCGLTFIHPRRFRSRVLVQYGPPIVVSPQEPPTADAVRALTAELDGALRRL
                     TINAPDWETVRALDTVRRLYQPLEISIGDRVELARRFNTHYGAVAADPRVVELMRRVC
                     AYQVQLDVLGITDRELARDLSNREIAARMMRHVTLLGFWLPLMVPGAPLHVPAVAFAR
                     IAGPRLTPRKDVIATTKLLSGMLLVLAAYAAAVAVLWWRAGVPAALAAAIVLPLSGIA
                     TLRVLDRAHLVRRGFGVLVRRLRFRRAVAALRRDRAQLVGDVIRVVTEVKPATLPALF
                     PRREQQHEEADVPRRAPSNAERDAEPEPPP"
     gene            2760..>3200
                     /locus_tag="VF516_28415"
     CDS             2760..>3200
                     /locus_tag="VF516_28415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111701.1"
                     /translation="MKLTAQPSQPVTPTEHLVVVGPSAIAATIEAFAAGAALDWAEQV
                     VVVASAPAARQLAAAGAAARLGLNVDPLVDALRAAGLPDRPTGAAYVRLVDRTGVPDH
                     IRTPFGLLIYGRQGVRNQGGMFVESSLDLVVAVIEPGRDPILLDD"
BASE COUNT          488 a         1095 c         1187 g          430 t
ORIGIN      
        1 cggcgacctc gcgcgcgcgc tcgacgacga gcgcgccgcc gacgaacgct cacagtgagc
       61 ggcggcgcgc cgccagcgcg accgacgcgg tgccgaacgc cgacccgccg tggcggctcg
      121 tcgacgtgcc gtgatcaacg cattccgcga gggaagtccg gcctggatgc tcgaggcgcg
      181 cggttcagaa gctgccgtgg cgcaggtagc gcacgaccag gccgtgtacg catgggtcgt
      241 tcatgatcca cgtgtgcgtc gcgtcgacct cggcgaagtc cgccatgccg tcgagccggg
      301 tctcggcgac cgcgaccgtg ccgtcgttcg gaacacccgg cggaaaccgg cgcccggcgg
      361 tccagctggt cgggttgatc agcgcgcgtg ctcgcgtgcc ggcgatcacc gcgaacggtg
      421 cgggcggcgc aggccagccg ctcgcgtcgg cgagctcgcg cccggcgggc ccgtagaacc
      481 agcgaaacag cgggttgatc gccagatcgc gggcgagccg gctgccctgg ttcggcggcg
      541 ccagcatcac gatgcgctgc catgcaatgc gcggatcgtg gaggtggcgt gccacgacgc
      601 cgcccagcga gtgagtcacg gcgctgatcg gccggccggc cgcctcgcgc gcgatcgtgt
      661 cggtcagcgc gcgcgcgatc tcggcgatcg aggcgcgccg cgacgggtag ctgtggctcc
      721 gggtgtcgaa gccgtgtgcg gcgagatggg cgccgagcct ggccatcgag ccggggccgc
      781 gagccaggcc atgcaacagc accacgagcg gcttcacggc ggcggctcgg gctcggcgtc
      841 gcgctcggcg ttcgacggtg cgcgccgcgg tacgtcggct tcctcgtgct gctgctctcg
      901 acgcggaaac agcgcgggca gcgtcgctgg cttgacctcg gtgaccacgc ggatcacgtc
      961 gccgacgagc tgcgcgcggt cgcggcgcag ggcggcgacc gcgcggcgga acctgagccg
     1021 gcgcaccagc acgccgaagc cgcggcggac gaggtgcgca cggtcgagca cgcgcagcgt
     1081 cgcgatcccc gacagcggta gcacgatcgc ggcggccagc gcggctggaa ccccggcacg
     1141 ccaccacagc acggcgaccg cggcagcgta cgccgcgagg acgagcaaca tgccgctcag
     1201 gagcttggtg gtcgcgatga cgtcctttcg cggcgtgagg cgaggtccgg cgatccgcgc
     1261 gaacgccacg gcgggtacgt gcagcggtgc accgggcacc atgagtggta gccagaaccc
     1321 gagcagcgtg acgtgccgca tcatgcgagc cgcgatctcg cggttcgaga ggtcgcgtgc
     1381 gagctcgcgg tcggtgatcc cgagcacgtc gagctggacc tggtaggcgc acacccggcg
     1441 catgagctcg acgacacgcg ggtcggctgc gaccgcgcca tagtgggtgt tgaaccgccg
     1501 cgcgagctcg acgcggtcac cgatcgagat ctcgagcggc tggtagaggc ggcgcacggt
     1561 atcgagcgcg cggacggttt cccagtccgg tgcattgatc gtgaggcgac gcagcgcgcc
     1621 atcgagctcg gcggtcagcg cgcgaaccgc gtcggcggtc ggtggctctt gtggggagac
     1681 cacgatcggc gggccgtact gcaccagcac gcggctgcgg aaccgcctgg gatgaatgaa
     1741 cgtgaggccg cacgggacga tcgagatcgg cgccgtcgcg cggtgggcgc cgccgagcgc
     1801 gagccgcgcc gcgccggtct tgagcctggc cagctgcgag tcgtcatgcg acaggccctc
     1861 gggaaagatc ccgatcgcgc cgccggactc gagcaccgcg aacatcgcgg caaacgcgga
     1921 ctcgttgtcg agcgcaggcg cggatacggg ttgcgccggg gcatcgcgcg gcggcgtgcc
     1981 atcgcgatcg tcgcgccgcc ggatcgggac ggcgcccaga ccgcgcagca cggcgcgcat
     2041 caagcggccc ttgaacagcg tgtccttggc cgcaaagtga accttgcggc cgcacgacgt
     2101 gatgatgagg atcggatcga tcagcgagtt cggatgattg ccggcaaaca gcacgccgcc
     2161 cgtcggcgga atgttctcga ccccgacgat ctcgacctgg cggaagaaca cgcgggtcac
     2221 gacgcggagg aaccacgcca ggatccggta caccacctcg gcatggtacc aggaatggcc
     2281 accacggcgt gaaccgctcc gccggctggt tcggtgtcgc ggagcgtcaa gacacagctg
     2341 ctccgggatt cctcctccga ggcccgtgct ggcgctcggc gccccacggt cgcccccgcg
     2401 ccgccatgtc gtggatgagg tcgatcgccc gctcgctggc gctgacccgg atcgatgcgg
     2461 cgctcgaatg atggagccgc gcgatcgacc gctggtgcaa gcatcatctc tcgacagaaa
     2521 cttctcatcg tacccggaac cgcatcgacg ggcccaccag gcagcgccgc gaagcgcggc
     2581 agccccttca tacacgttca cacgatgtgg taccgaggca gcgacgatga ccatgataca
     2641 ttcgtcggtg atgcagcggt tcgacggtcg cggccgaaga gctgtggcgg cgcgtcaagg
     2701 cggagcccgc tccgaggacc atcgcggcgg cgctggggtc gatcgcgagc gcgtggggcg
     2761 tgaagctgac cgcgcagccg agccagccgg tcaccccgac cgagcatctc gtggtcgtgg
     2821 ggccgtcggc gatcgcggcc acgatcgaag cattcgcggc cggcgctgcg ctggactggg
     2881 ccgagcaggt cgtcgtggtt gcgagcgcac cagccgcccg gcagctggca gcggctgggg
     2941 cggcagcccg gctcgggctg aacgtggatc cgctggttga cgcgctgcgg gcggcgggtc
     3001 tccccgatcg cccgactgga gcagcgtacg ttcgcctcgt cgaccggacc ggcgttcccg
     3061 accacatccg cacgcccttc gggttgctga tctatggccg ccagggcgtt cgcaatcagg
     3121 gaggtatgtt cgtcgagagc agcctcgacc tcgtcgtcgc ggtgatcgag ccggggcgag
     3181 atcccatcct gctcgatgac
//
LOCUS       DASVOR010001505         5390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_220035,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001505 DASVOR010000000
VERSION     DASVOR010001505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220035"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VF516_28420"
     CDS             <1..467
                     /locus_tag="VF516_28420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111702.1"
                     /translation="VRDRLAHLSDPRTRSVALVVAEVAIGETISMAMFRESRRVATEP
                     LTRAAIESILADESRHQQLGWDALAALGPGDDVQREATLGLAASEQQIAVPALRFLEA
                     GQAFDPAWAALGVVEPGRRVDAFYTAVEQLVIPKLDQLGLDGARAWSYRYRA"
     gene            520..1599
                     /locus_tag="VF516_28425"
     CDS             520..1599
                     /locus_tag="VF516_28425"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8111703.1"
                     /translation="MSHDSFSERVALAVLHGALAPVATPALLHVGELGGWPYLVMTRL
                     DGTPLEDVWPGAAAGEQAQLLEQIGALIAQVHALDPGPIAAIGPRWDDFVAAQRAGCV
                     ARHTRLALPAPLLAELDAYLDRVAPAIPRAIAPVILTGEYTPANLLVTRRAGRWQITG
                     LIDFGDVGCGAPDYDLLGPATFLAAGDPAHLRALLVARGYSPGELVPALRDRLMALLG
                     VCSDADYLLSRLRVLGWGWHHGFTHSVVFAVLVGATVRWGLGLRGWRGALACVLPVLS
                     HPLLDYVVTESPGVALWWPVTGRRTKLGIDALSYYHLIGASEGALAFVRLCAAEVVLF
                     GPIFAVAVLASRQRFVSYLSPGSTR"
     gene            complement(1623..2198)
                     /locus_tag="VF516_28430"
     CDS             complement(1623..2198)
                     /locus_tag="VF516_28430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8111704.1"
                     /translation="MALTRDEAWTLLCDWTRGDSLRRHARAVELVMRAAAPRYGEPGA
                     DVEAWGIAGMLHDADYEAWPDEHPHRIVAWLRERGEDAIAHAVSAHYTRWGVPYDSRL
                     DRALLACDEPTGFVMACCLVRPDGIASLEPASVKKRLKDKRFAATVDRAEVTAGAELL
                     GVPLDDHLGFVIDALRPHAAELGLLGRSAPA"
     gene            2252..2740
                     /locus_tag="VF516_28435"
     CDS             2252..2740
                     /locus_tag="VF516_28435"
                     /inference="COORDINATES: protein motif:HMM:NF017747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutD family protein"
                     /protein_id="HEX8111705.1"
                     /translation="MQRIVAPAEFRTQPWKNGGGVTHEIIRWPDHEAYEVRISLAEDR
                     VPGPFSQFPGYRRWSFLAGTAPIVLHVAGGVHELVALGDQIEVAGDVAIRCTLPAGPT
                     RLLNVLVREGVDARIGRGPCSLAVRFVFALAAMPWLPEGHAAVFDPPEIVGLERHAVW
                     LA"
     gene            3112..3546
                     /locus_tag="VF516_28440"
     CDS             3112..3546
                     /locus_tag="VF516_28440"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEX8111706.1"
                     /translation="MSNETTYKGSCFCGAVELTVTGQPVAMGYCHCSSCRSWSAGPVN
                     AFMLWKPESVKITKGASDIGTYNKTPVSFRKWCLKCGGHVMTDHPPFGLIDVYAATIP
                     SFPFQPALHVHYGESVLRIKDGLPKQKDMPSEMGGSGTLLAE"
     gene            3876..4871
                     /locus_tag="VF516_28445"
     CDS             3876..4871
                     /locus_tag="VF516_28445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111707.1"
                     /translation="MFLSVLTFGCTTRAGDRVDGQGIAELRVGAASLGITGITRVTVQ
                     SADVSQDLVFHEDDSAYEGTVILPAGTVTLVASAFNGDALVGQSRPTPVEITRGAVTR
                     VFLQILDLRQSGPPRFGPIFDSLSFPTTVTAGDVATFTIAVIAPAGDPVTYAWTSDCT
                     DATFTAPDAATTGWSKPTQGICHVRVVATSNGLSVTQNFSIVVFPASDSGAVDVTAGF
                     VFAPAVAFQISGAGCLALDQDASCPTPIASPTVVNYSLSVFSWNSFPGTGTFTVSDNC
                     GGTFFPEFQNRDFVTGQWLPPVTAGICILTARAVNDPGAVTIRGAAIVVHAGTAP"
     gene            complement(4907..>5390)
                     /locus_tag="VF516_28450"
     CDS             complement(4907..>5390)
                     /locus_tag="VF516_28450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111708.1"
                     /translation="GIGNVWAIAETPAGLWLGTTTGLYRGPATPWTSKDGSDDDAALA
                     DRWQRLSVASGQLRDDWVTALVTRGDVVWAGTYNGGVARIDGDTATPLGGGWVNPSGL
                     IWDGDHLLAATMDGLVTGDGRTATWTATPGLPGRDVTAAVRTGKTLWVATRRGLIGLD
                     "
BASE COUNT          703 a         1922 c         1912 g          853 t
ORIGIN      
        1 cggtccgcga tcgcctcgcg cacctgtccg atccgcggac gcgcagcgtc gcgctcgtcg
       61 tcgccgaggt cgcgatcggc gagacgatct cgatggcgat gttccgcgag agccgccggg
      121 tggcgaccga gccgctgacc cgcgctgcga tcgaatcgat cctcgccgac gagtcgcgcc
      181 accagcagct gggatgggac gcgctcgccg cgctcggccc cggcgacgac gtccagcgcg
      241 aggcgacgct cgggctcgcc gcctccgagc agcagatcgc cgtgcccgcg ctgcggttcc
      301 tcgaggccgg ccaggcgttc gatccggcgt gggcggcgct cggcgtcgtc gagccaggcc
      361 ggcgcgtcga cgcgttctac accgccgtcg agcagctggt gatcccgaag ctcgatcagc
      421 tcggcctcga cggcgcgcgc gcgtggtcgt accgctatcg agcgtgaccc ggcccgggcc
      481 gccggtcact gcggcgcgat cgcgatcgtc gcgacggtct tgtcccacga cagcttcagc
      541 gagcgcgtgg ccctcgccgt cctgcacggc gcgctcgcgc cggtcgccac gcccgcgctc
      601 ctgcacgtgg gcgagctcgg cggctggccg tacctggtca tgacgcggct cgacggcact
      661 ccgctcgagg acgtgtggcc gggggccgcg gccggcgagc aggcccagct gctcgagcag
      721 atcggcgcgc tgatcgcgca ggtccacgcg ctcgatcccg gtccgatcgc ggcgatcgga
      781 ccgcgctggg acgacttcgt cgccgcgcag cgcgccggct gcgtcgcgcg ccacacacgg
      841 ctcgccctgc cggcgccgct gctggccgag ctcgacgcgt acctcgatcg cgtcgcgccg
      901 gcgatcccgc gcgccatcgc gcccgtgatc ctgaccggcg agtacacgcc ggcgaacctg
      961 ctcgtcacgc gccgcgccgg tcgctggcag atcaccggcc tgatcgactt cggagacgtg
     1021 gggtgcggcg cccccgacta cgatctgctc ggacccgcga cgttcctcgc cgccggcgat
     1081 ccggcccacc tccgcgcgct gctcgttgcc cgcgggtact cgcccggcga gctggtcccc
     1141 gcgctgcgcg atcggctgat ggcgctgctc ggcgtgtgct ccgacgcgga ctacctgctc
     1201 agccggctgc gcgttctggg ctggggctgg caccacggct tcacccactc ggtcgtgttc
     1261 gcggtgctcg tcggcgcgac cgtgcgctgg ggactcggcc tccggggatg gcgaggcgcg
     1321 ctcgcgtgcg tgctcccggt gctctcccat ccactgctcg actatgtggt cacggagtcg
     1381 ccgggagtgg cgctgtggtg gcccgtcacg ggtcggcgga ccaagctcgg gatcgatgcg
     1441 ctgtcgtact accacctcat cggggcgtcg gagggcgcgc tcgcgttcgt gagactctgc
     1501 gcggcggagg tcgtcctgtt cggtccgatc ttcgcggtcg cggtgctcgc gagccgccag
     1561 cgcttcgtga gctacctgtc gccgggctcc acccgctgag cgttcgcgat ccccgcgtcg
     1621 gatcaggccg gcgcgctgcg gccgagcagg ccgagctcgg cggcatgggg gcgcaaggcg
     1681 tcgatcacga acccgagatg gtcgtcgagc ggtacgccga gcagctcggc gccggccgtg
     1741 acctcggcgc ggtcgacggt ggcggcgaac cgcttgtcct tgaggcgctt cttcaccgac
     1801 gccggctcca ggctggcgat gccgtcgggg cggaccaggc agcacgccat cacgaagccg
     1861 gtcggctcgt cgcacgcgag cagggcccgg tcgagccggc tgtcgtaggg gacgccccag
     1921 cgggtgtagt gggccgacac cgcgtgggcg atcgcgtcct cgccgcgctc gcgcagccac
     1981 gcgacgatcc gatgcgggtg ctcgtcgggc cacgcctcgt agtcggcgtc gtgcagcatg
     2041 ccggcgatgc cccacgcctc gacgtcggcg ccgggctcgc cgtagcgcgg cgcggccgcg
     2101 cgcatcacca gctcgaccgc gcgagcgtgg cggcgcagcg aatcgccccg ggtccagtcg
     2161 cacagcaagg tccaggcttc atcgcgggtc agcgccatgg cgccgagcat atccgtattg
     2221 tggtacagcc gccggaaccg tacgcttcag catgcagcgc atcgtggcgc ctgccgagtt
     2281 ccgtacccaa ccatggaaga acggcggggg tgtgacccac gagatcatcc gctggccgga
     2341 ccacgaggcc tacgaggtcc ggatcagcct ggcggaggac cgggtgcccg ggccgttctc
     2401 acagttcccc gggtaccggc ggtggtcgtt cctcgcgggg accgcgccga tcgtgctgca
     2461 tgtggccggg ggcgtgcacg agctcgtggc gctcggcgat cagatcgagg tcgccggcga
     2521 tgtcgcgatt cgctgcacgc tgccggctgg accgacccgg ctgctcaatg tcctggtgcg
     2581 cgagggcgtc gacgcgcgga tcggtcgcgg gccgtgctcg ctggcggtgc ggttcgtgtt
     2641 cgcgctggct gcgatgccgt ggttgcccga gggccacgcg gcggtgttcg acccgcccga
     2701 gatcgtcggg ctggagcgcc atgccgtgtg gctcgcgtga ccgaggcgcg gctgactgcc
     2761 ccggggcacg ccttcagcga ccggtggtct cggtcaaggt cagcgatccg aagtactcga
     2821 accaggccaa ggacaagctg gtgtcgtccg aggtcgcgac cgcggtgtcg ccgtcgatcc
     2881 gggcggcgcc gccacctcga ccgcttcgag cacgccgccg cacttcgccg agagcgacgc
     2941 gtccaggacc gtcaccttcc tgccctgggc gccgcggtag cgcggcggca gagccttcgg
     3001 atcgatcgcg cagcctttac cagctcacgc ctgccatggt agttgcctca ggaagccgca
     3061 tcggcccatg cgattccacg cggccgtgac gacaacctgg ggagatgcca gatgagcaat
     3121 gaaacgacgt acaagggaag ctgcttctgc ggagccgtcg agctgacggt gaccggccaa
     3181 cccgtggcca tgggatactg ccattgcagc tcctgccgca gctggtccgc cgggccagtc
     3241 aacgcgttca tgctgtggaa gccggagtcc gtgaagatca ccaagggcgc gagcgacatc
     3301 ggtacctaca acaagacgcc ggtgagcttc cgcaaatggt gcctgaaatg cggtggccac
     3361 gtcatgaccg atcacccgcc gttcgggttg atcgacgtgt atgccgcgac catcccgagc
     3421 ttcccgttcc agcctgcgct gcacgtgcac tacggagaat cggtgctccg gatcaaggat
     3481 ggtctcccca agcagaagga catgccgagc gagatgggtg gttcgggcac gctgctcgcc
     3541 gagtgacctc gcgccgcatc ggcgtccggt ggggctgtgt gcagcgccgc caccgcgcct
     3601 cccggcggcc catcgcatgg ttccggtgtt gtgatgcgac cgcatcgcag aacagtcgga
     3661 acctatgcgg aacacgtcgg aatgtaccga tcatcggatc tctgcactgc tatcgaattt
     3721 caaatccgga atgccatccg cggtttgccg tggaaggccc gtttgttccg gtcgtgtccc
     3781 tggaggggtc tcccgccgga tgctacgagc cgggctgcgc ttgcaacccg agcgcttcat
     3841 ccaaggagac cttcgtgcgc gcttatctgt ccgtcgtgtt cctctctgtc ctgacgttcg
     3901 gctgtaccac ccgtgccggc gatcgcgtcg atggccaggg gatcgccgag ctgcgggtcg
     3961 gcgccgcctc gctgggcatc accggcatca cgcgggtgac cgtccagtcc gcggatgtgt
     4021 cccaggacct cgtgttccat gaagacgatt ccgcgtacga gggcaccgtg atcctgcccg
     4081 cgggcacggt gaccctggtc gccagcgcgt tcaacggcga cgcgctggtc ggccagagcc
     4141 ggccgacccc ggtcgagatc acccgcggcg cggtcacccg cgtgttcctg cagatcctcg
     4201 atctgcggca gagcggcccg ccgcggttcg ggccgatctt cgattcactg tcgttcccca
     4261 ccaccgtcac ggccggggac gtggccacct tcacgatcgc ggtgatcgcc ccggccggcg
     4321 acccggtgac ctatgcgtgg acctcggact gtaccgacgc gacgttcacc gcgcccgacg
     4381 cagccaccac gggctggtcc aaaccgacgc agggcatctg ccatgtcagg gtggttgcca
     4441 cgtccaacgg tctgagcgtc acccagaact tctcgatcgt cgtgttcccg gcgagcgaca
     4501 gcggcgcggt cgatgtcacc gccgggttcg tgttcgcccc ggcagtcgcg ttccagatca
     4561 gcggcgcggg ctgcctcgcc ctcgatcagg acgcgtcgtg cccgacgccg atcgcctcac
     4621 ccaccgtggt caattactcc ctgtcggtgt tcagctggaa cagcttcccc ggcaccggca
     4681 cgttcacggt ctcggacaac tgcggtggaa cgttcttccc ggagttccag aaccgcgact
     4741 tcgtcacggg ccagtggctg ccacccgtca ccgcggggat ctgcatcctc accgcgcgcg
     4801 cggtcaacga tcccggcgcg gtcacgattc gcggggcggc catcgtcgtc cacgccggca
     4861 ccgcaccctg atccgccgac atgggcggcg cgtggaccgg ttccggtcag tccaggccga
     4921 tcaggccgcg gcgggtggcg acccacaggg tcttgccggt gcggacggcg gccgtgacgt
     4981 cgcggccggg gaggccgggc gtggcggtcc aggtggcggt gcggccgtcg ccggtgacca
     5041 ggccgtccat ggtcgccgcg agcaggtgat cgccgtccca gatcaggccc gacgggttga
     5101 cccagccgcc gccgagcggg gtcgcggtgt cgccgtcgat ccgggcgact ccgccgttgt
     5161 aggtgccggc ccagacgacg tcgccgcggg tgacgagggc ggtgacccag tcgtcgcgga
     5221 gctggccgct ggcgaccgac agccgctgcc agcggtcggc gagggcggca tcgtcgtcgc
     5281 tgccgtcctt ggaggtccac ggcgtggcgg ggccgcggta gaggccggtg gtggtgccga
     5341 gccagaggcc cgccggggtc tcggcgatcg cccacacgtt gccgatgccg
//
LOCUS       DASVOR010001506         3498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_277500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001506 DASVOR010000000
VERSION     DASVOR010001506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            282..1310
                     /locus_tag="VF516_28455"
     CDS             282..1310
                     /locus_tag="VF516_28455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111709.1"
                     /translation="MSSLRFSLLAVLVAACGPRPQPIVYLPIEPAPPGEDLVATTEPP
                     TATETAATETTPKPAGPAPSSSWPDYQVPTLPMDLVLHRGTLVWADLAGALWTMPADG
                     SGAPKQVSEQHRDGFAAHPFVAGDRVMAKGGKGLLAIEVPGGAVTSVHISGVPDLLED
                     VVGDASTIFFTVFKHDQVMRVPVTGGAAQHVLDAKSSVLALHDPMLYVASYDTGDLLA
                     VPTAGGVPRTIARKLKQPTALAVEDTAAYVYTEGDQRLTRVELTTGATEVLGEHLDNS
                     DEIELAADALYTVSWPNKLVRLPKVPGAAPATLTDKLFQPRGVVRDEHFIYVTSDQPP
                     RIVRVPAR"
     gene            1317..1466
                     /locus_tag="VF516_28460"
     CDS             1317..1466
                     /locus_tag="VF516_28460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111710.1"
                     /translation="MTDVRTPSSFATVTVNTRAGYRSSSTALANLAAVAETHSDDLGP
                     LRKGA"
     gene            1536..3155
                     /locus_tag="VF516_28465"
     CDS             1536..3155
                     /locus_tag="VF516_28465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111711.1"
                     /translation="MRIRRPWLIARGSSAAHRRAELFFEDLTGEAASAGEEAPGAIAS
                     FEEIFGEGIDVPWSGIEADQAIEADPPKAVNLPRTTITAGASRTEKLALARAKTLSDV
                     NSALATTHPLGDYTKYKGMSYAGGKAKVPEPSEVESRRTKLAEAIDAVSRQDFSTAIS
                     KVRELALPLPEVDAVLATDQFLEAQWLTAFSLGMIKFGKSFEFDNTDPPLVAVQGFAH
                     YVSLLNAMAMRGVTGLKSPPTNENLLAYLFNLRIEVGHLPAQEQRIILSRGHQMVMNG
                     FVVHYALASGKDPIYPEVTQDSWYVFEPPKGDVQTGAGRAWRYPSKESAQKGVVTRGS
                     KLWQVIQTVGKKTSKPTLPDSFGDIVAQDTYGYRYESNCAGMASLRVRTMPPGFEPVG
                     CVHGTLRKDLSRPAHIVLVYSDRGYEDDPWPAPANLYMSSNGKPPILIKRGSDGAYTA
                     KIIQDAVEAEFGNTYRGSPSGSEFAFGIGVPTYPPGVRMLTGSPDFATRMNELNHVAD
                     RILLSAYADNAVRFRNPKNVPDVPWNLLVIR"
     gene            3155..>3498
                     /locus_tag="VF516_28470"
     CDS             3155..>3498
                     /locus_tag="VF516_28470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111712.1"
                     /translation="MNTPLGALLAALLLSLSAPTAAASSDDPARRRRAQGTSSAVTAP
                     PDRRGRRGPVLGRRRLGRVSLSGCEVYGQKIEIDATARQLPDRRAVTVTNVARSDARR
                     VRRVRVRRVRVHR"
BASE COUNT          553 a         1289 c         1138 g          518 t
ORIGIN      
        1 cggcgcggcg ggcggcgccg cggccggcgg cgatgcacag gcggcgagcg cgagcgccag
       61 acaggcacgg tgggacatcg ccacgagtat gcatctgacg gcaccacctg cacgcctcgg
      121 ctcggccgtc aggtcatcac gccgccggag acaccgtcat tgacggcccg gcgggtgacg
      181 cgctcgagtc acactatctt gaatcagatc ggtgacggcg agcgccgcag tcatcggccg
      241 agcttggtcc ggaacgcgcc cgtgcgctac catcggcggc ggtgtcatcg ttgcgcttct
      301 cgctgcttgc cgtgctcgtc gccgcgtgcg gtccgagacc gcagccgatc gtctacctgc
      361 ccatcgagcc cgcgccgcct ggcgaggacc tggtcgcgac gaccgagccc cccacggcga
      421 cggagaccgc cgccaccgag accacgccga agccggccgg acccgcgccc tcgtcgtcgt
      481 ggcccgacta ccaggtcccg acgctcccga tggatctcgt tctgcaccgc ggcaccctgg
      541 tgtgggcgga tctcgccggc gcgctctgga cgatgccggc cgacggatcc ggcgcgccga
      601 agcaggtctc ggagcagcat cgcgacggct tcgccgcgca cccgttcgtc gccggcgacc
      661 gcgtgatggc caagggcggc aagggtctgc tcgcgatcga ggtgcccggc ggcgcggtga
      721 cctccgtcca catctccggc gtgcccgacc tgctcgagga cgtcgtcggc gacgcgtcga
      781 cgatcttctt caccgtgttc aagcacgatc aggtcatgcg cgtgccggtc acgggcggcg
      841 cagcgcagca cgtgctcgac gccaagagct cggtgctcgc gctccacgac ccgatgctct
      901 acgtcgccag ctacgacacc ggtgatctgc tcgcggtgcc gaccgccggc ggcgtgccgc
      961 gcacgatcgc caggaagctc aagcagccga ccgcgctcgc cgtcgaggac accgccgcgt
     1021 acgtctacac cgagggcgac cagcggctga ctcgcgtcga gctcacgacc ggcgcgaccg
     1081 aggtcctcgg tgagcacctc gacaacagcg atgagatcga gctcgcggcc gacgctctct
     1141 acaccgtgag ctggccgaac aagctcgtcc gtctgcccaa ggtcccgggt gccgcgcccg
     1201 cgacgctgac cgacaagctg ttccagccgc gcggcgtcgt gcgcgacgag cacttcatct
     1261 acgtgacgtc cgaccagccg ccgcgcatcg tgcgcgtccc cgcgcgctga atgatcgtga
     1321 ccgatgtgcg aaccccgtcg agcttcgcga cggtcaccgt caacacccgt gctggctacc
     1381 gatcgtcctc gacggccctg gccaacctgg ccgccgtcgc ggagacccac tccgacgacc
     1441 tggggcccct ccgcaagggc gcttgacttc ggtgaaattt cttgcttttc tgtctaccct
     1501 cgagcgtccg tgtggtcgcg ccggaggggg gactgatgcg cattcggaga ccatggctca
     1561 tcgcgcgggg cagctcggcg gctcaccgcc gagcagagct gttcttcgag gatctgaccg
     1621 gcgaggccgc gtccgccggc gaggaggcgc ccggcgcgat cgcctcgttc gaggaaattt
     1681 tcggcgaagg tatcgacgtc ccgtggtcgg ggatcgaggc ggaccaggcg atcgaggcgg
     1741 acccgccgaa ggccgtcaat ctcccccgca ccacgatcac cgcgggggcc tcgcgcaccg
     1801 agaagctcgc gctcgctcgg gcgaagacgc tgagcgacgt gaactccgcg ctcgcgacga
     1861 cgcacccgct cggcgactac acgaagtaca agggcatgag ctacgccggc ggcaaggcca
     1921 aggtgccgga gccgagcgag gtcgagagcc gccgtaccaa gctggcggag gccatcgacg
     1981 ccgtgagccg gcaggacttc tccaccgcga tctcgaaggt ccgcgagctc gccctcccgc
     2041 tccccgaggt ggacgcggtg ctggcgacgg accagttcct cgaagcgcag tggctcaccg
     2101 cgttttcgct cggcatgatc aaattcggca agagcttcga gttcgacaac acggatccgc
     2161 cgctcgtggc cgtccagggc ttcgcccact acgtctcgtt gctgaacgcc atggccatgc
     2221 gcggcgtcac cggcctcaag tcgcctccga cgaacgagaa cctgctcgcc tatctcttca
     2281 acctgcgtat cgaggtgggc cacctacccg cccaagaaca gcggatcatc ctctccagag
     2341 ggcaccagat ggtcatgaac ggcttcgtcg ttcattatgc cctcgcgagc ggcaaggacc
     2401 ctatttatcc cgaggtgaca caggatagct ggtacgtctt cgagcccccc aaaggggacg
     2461 tgcagacggg ggccggcagg gcgtggcgct acccctccaa ggagagcgcc cagaagggag
     2521 tcgtcacgcg tggatccaag ctctggcagg tgatccagac ggtcggcaag aagacgagca
     2581 agccgacgct gccggactcg ttcggcgaca tcgtcgcgca ggacacgtac ggctatcggt
     2641 acgagtcgaa ctgcgcgggg atggcctcgc tgcgcgtccg caccatgccc cccggcttcg
     2701 agccggtggg ctgcgtccac ggcacgctcc gcaaggacct cagccggccg gcccacatcg
     2761 tcctggtcta ctcggatcgc ggctacgaag acgacccctg gcccgccccg gcgaacctct
     2821 acatgtcgtc caacggcaag ccgcccatcc tcatcaagcg cgggagcgac ggcgcctaca
     2881 cggcgaagat catccaggac gccgtggagg ccgagttcgg caacacgtac cgcggcagcc
     2941 cctcgggatc ggagttcgcc ttcgggatcg gggtcccgac ctatcccccg ggcgtgcgca
     3001 tgctcaccgg ctcgcccgat ttcgccacgc ggatgaacga gctgaaccac gtcgccgatc
     3061 gcatcttgct cagcgcgtac gccgacaacg ccgttcgctt ccgcaacccc aagaacgtgc
     3121 cggacgtccc ctggaatctg ctcgtcatcc gctgatgaac acgccgctcg gcgctcttct
     3181 cgcggcgctc ctgctctctc tctccgcccc caccgccgct gcctcctccg acgaccccgc
     3241 gcgccgccgt cgcgcccaag gcacctcctc cgccgtcacc gctcctccag atcgccgcgg
     3301 acggcggggt cctgttctgg gacgtcggcg gctcgggcga gtgtcactgt ccggctgcga
     3361 ggtgtacggg cagaagatcg agatcgacgc gaccgcccgc cagctcccgg accgccgcgc
     3421 cgtgacggtc acgaacgtcg cgcgctcgga cgcacgacgc gtgcgccggg ttcgcgtgcg
     3481 ccgggtccgg gtgcaccg
//
LOCUS       DASVOR010001507         2135 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_249396,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001507 DASVOR010000000
VERSION     DASVOR010001507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2135
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249396"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1085)
                     /locus_tag="VF516_28475"
     CDS             complement(<1..1085)
                     /locus_tag="VF516_28475"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111713.1"
                     /translation="MKKPTPFGKYYLLERISIGGMAEVFRAKAFGVEGFERLVAVKRI
                     LPNIAEDKEFIRMFIEEAKLSVQLNHANIAQIFDLGVVEGSYYIALEHVHGRDLRVLF
                     ERCHQLGEAMPIAQACFIAMKVCEGLDYAHNKRDQAGRELSLVHRDVSPQNILVSFEG
                     EVKLIDFGSAKAAGTGASGQAPILKGKFGYMSPEQVRGVEIDRRSDVFSCGVVLYELL
                     TGERLFVGESDFDTLEKVRNVEILPPSTYNRKIPDELERIVLKALSKDAEERYQNAID
                     LHDELQAFVYTAGEFYSRKDLAGWMKKTFGKEIEDETAKLESYRQLKPPPPSAVPPPP
                     PGAADGVPEPARTQQSQSGRLPRKPPT"
     gene            complement(1223..>2135)
                     /locus_tag="VF516_28480"
     CDS             complement(1223..>2135)
                     /locus_tag="VF516_28480"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8111714.1"
                     /translation="PERYYCSEVDQTCHHRVPVVMSPTMIELYNGQFAKSHGLPIADV
                     DFVKFVTARGGLGAMRFSIGLGLTTIAGSNAGAARKARRVEAVLVGINPKAMPIGMTM
                     PIQYIQRWNREFLGDEAATAYSSIIVRLKDRNRIAVFSQWLQDDLDLRLEDSLGEKFA
                     TALFVIRLVLVLISLVIITISSINIAHNFFMQVTERRRELGLLRAVGATQSDVLLVVL
                     GEAALIGLIGGLIGLAIGVGLAAGIDWGSAHYLPRFPYKPSSWFHFKWWIVAGGLLCS
                     TGFSVLGGYLPARRASTMEPAQALTQN"
BASE COUNT          348 a          710 c          715 g          362 t
ORIGIN      
        1 tgggtcggcg gcttgcgcgg cagccttccg gactgcgact gctgggtgcg cgccggctcg
       61 ggcacaccgt ccgcggcccc gggcgggggc ggcggcacgg ccgagggagg cggcggcttg
      121 agctggcggt agctctccag cttggcagtc tcgtcctcga tctccttgcc gaacgtcttc
      181 ttcatccacc cggcgagatc cttgcgcgag tagaactcgc cggcggtgta gacgaacgcc
      241 tggagctcgt cgtggaggtc gatcgcgttc tggtagcgct cctcggcgtc cttgctcagc
      301 gccttgagca cgatgcgctc gagctcgtcg gggatcttgc ggttgtaggt cgacggcggg
      361 aggatctcga cgttgcggac cttctccagc gtgtcgaagt cgctctcgcc gacgaacagc
      421 cgctcgccgg tgagcagctc gtagagcacg acgccgcacg agaacacgtc gctgcggcgg
      481 tcgatctcca cgccgcgcac ctgctcgggc gacatgtagc cgaacttgcc cttgaggatc
      541 ggggcctggc cgctcgcgcc ggtgccggcg gccttcgcgc tcccgaagtc gatcagcttc
      601 acctcgccct cgaacgacac caggatgttc tgggggctga cgtcgcggtg gaccagcgac
      661 agctcgcggc cggcctgatc gcgcttgttg tgcgcgtagt cgaggccctc gcacaccttc
      721 atcgcgatga agcaggcctg ggcgatcggc atcgcctcgc cgagctggtg gcagcgctcg
      781 aacagcacgc ggaggtcgcg gccgtggacg tgctcgagcg cgatgtagta gctgccctcg
      841 accacgccga ggtcgaagat ctgggcgatg ttggcgtggt tgagctggac cgacagcttg
      901 gcctcctcga tgaacatgcg gatgaactcc ttgtcttccg cgatgttggg gaggatccgc
      961 ttgaccgcga cgaggcgctc gaagccctcg acaccgaagg ccttggcgcg aaacacctcg
     1021 gccatgccac cgatgctgat ccgctccagg aggtagtact tgccgaaagg ggtcggcttt
     1081 ttcacaggtc gttaggcccc cagcccaggc tggcgaagat tccgtgcacc cagcagcagc
     1141 ttagcgtgca gaggagcgtt gtcgtactcc aggggtttcg cgatttctac gaaaattctc
     1201 cagggttgct acccaccaaa cgttagttct gggtaagtgc ctgagctggc tccatcgtcg
     1261 aggcccgccg cgccggcaag tagccgccga gcaccgagaa cccggtcgag cacaacagcc
     1321 cccccgcgac gatccaccac ttgaagtgaa accagctgct cggcttgtac gggaaccgcg
     1381 gcaggtaatg ggcggatccc cagtcgatcc cggccgcgag gccaaccccg atcgcgagcc
     1441 cgatcagccc gccgatcagg ccgatcagcg ccgcctcgcc gagcacgacc agcagcacgt
     1501 cgctctgcgt ggcgccgacc gcgcgcagca ggccgagctc gcggcggcgc tcggtgacct
     1561 gcatgaaaaa attgtgggcg atattgatcg acgagatcgt gatgatcacg agcgagatca
     1621 gcaccagcac cagccggatc acgaacagcg ccgtcgcgaa cttctcgccc agcgagtcct
     1681 cgagccggag gtcgagatcg tcctgcagcc actgcgagaa caccgcgatc cggttgcggt
     1741 ccttgagccg gacgatgatc gacgagtagg cggtcgcggc ctcgtcgccg aggaactcgc
     1801 ggttccagcg ctggatgtac tggatcggca tggtcatgcc gatcggcatc gccttggggt
     1861 tgatcccgac cagcacggcc tcgacccggc gcgccttgcg ggccgccccc gcgttgctgc
     1921 cggcgatcgt cgtcagcccg aggccgatcg agaaccgcat cgcgcccagc ccgccgcgcg
     1981 cggtcacgaa cttgacgaag tcgacgtcgg cgatcggcag gccgtgcgac ttggcgaact
     2041 ggccgttgta cagctcgatc atcgtcggcg acatcacgac cggcacgcgg tggtggcagg
     2101 tctggtcgac ctcgctgcag tagtagcgct cgggc
//
LOCUS       DASVOR010001508         7552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_307825,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001508 DASVOR010000000
VERSION     DASVOR010001508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307825"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VF516_28485"
     CDS             <1..654
                     /locus_tag="VF516_28485"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8111715.1"
                     /translation="PPMRTEVRGTPPGLRPLPDLRPLPDLRPTPEPRPTPESRPAAPS
                     APAIPPNPRLAPQDIPPVPEPADKPADATSELVDALHRGHALFEHGDIAAAIEVYDAI
                     VRTHPSVAEPWLFLGIARYAHGDVEEAASALRASLCLDTTLWPAGFYLARAYERLGRR
                     ADALQQYDLIAVDDLQPFSLLSTSAVINELRAFGHDFRNAARRLSAERTATARRPFR"
     gene            949..3387
                     /locus_tag="VF516_28490"
     CDS             949..3387
                     /locus_tag="VF516_28490"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular metalloproteinase"
                     /protein_id="HEX8111716.1"
                     /translation="MIRNTRLIQLLGLLAFPASLSVTACATAIDDGDAPANERDKALE
                     LLQRNSGAPVTMELNEAGITRVVAMTPRFPVPTHATDVAQAATSFLASHHDVLQLDAA
                     DAASFVITRVDAEPERNMSHVTLQRTFNGIPVFQGAITVHMDGGNSVFRVLGDEFYRI
                     SAPTNRMMLSPDEAALAAGRALGVSLSLSASETNGQSAVFSSPGTLDPVKVDPQIVQV
                     SADDNRLAFRVLLSWLDDQKQQQYQLALVDAQDGALLGSHSLVNTFQGKVFNVNAQPT
                     ATLATDTRTEQSFDGDPAASPLGWVTTPGNKTIGNNAVAATDLDGNNTVGTNETQPTA
                     VNNAFDFPFDPTVDASNFKAAAVANAFFLVNDYHDRTYKLGFTEAAGNFQTNNNGKGG
                     AANDPVNVDSQDGSGTNNANFSTPPDGSRPRMQMFLFSLKNGTGGVRQDGDFDPSVIY
                     HENTHGLSNRLVGGGTTSCLGALQSGGMGEGWGDFMGSAFLNNPVVGAYVTGNATRGI
                     RQFPMNNSPFTYNDIKNGTLAEVHNVGELWAATLWDVRTAVGDAITQQLVVSGMKLTP
                     CNPTMLNARDAIIQADANLNAGNNRCKIWTAFANRLMGTGASSPNASSTTTIVTSNAV
                     PADCAGGGTGTTVFSDDFETDKGWTANPNGTDTATTGKWERGIPQATTSGGTQQVGTP
                     TSGTHDLVTGASAGASVGDNDIDGGTTSIRSPAIAIPATGTTTLSFKAYFAHLNNSTT
                     DDFLRVQVVGTTTTTVFQVLGSATQVNGAFAAQSANISAFAGQTVRILISAADAAGGS
                     LVEAGVDDILITNQ"
     gene            3519..4553
                     /locus_tag="VF516_28495"
     CDS             3519..4553
                     /locus_tag="VF516_28495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline iminopeptidase-family hydrolase"
                     /protein_id="HEX8111717.1"
                     /translation="MTRGLALAFAIALTLGLAACPAPQGPTLAGYFTSEPGLQAAGVK
                     RIPIHTPKGDFTVWTKRFGANPRIKLLLLHGGPGATHEYFEALENDLAAAGVEFIYYD
                     QLGSAYSDQPTDTSLWTLDRFVDEVEQVRVALGLDKDNFYLLGHSWGGILAVEYALAH
                     GDHLKGLIISNMMMSIPDYNKYADTVLAKQMPPAVVSEIKAIEARGEYESPRYMDLLI
                     PNFYHAHICRLTDWPDAVNRALGRINRQIYVLMQGPSEFGASGLLEKWDRKADLPKLT
                     MPTLVIGGAHDTMDPDHMRWVSTQVQHGSFLLCRNGSHLDMWDDQQTYIRGLLKFLDA
                     VDHGARSVTF"
     gene            5002..6183
                     /locus_tag="VF516_28500"
     CDS             5002..6183
                     /locus_tag="VF516_28500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111718.1"
                     /translation="MPIHTRGKSCGEIVQASYGSYLVNATADCAGCHSSSAGFLAGGN
                     PFPLDRKGHVVYSRNLTPDPTTGMRLTKEQFVEALRTGRDFHPGSAGILVVMPWLFYR
                     WASDGDLQAIYAYLRAIPPVKNFVPPDVKAGLGLPQAIPFTGIYDEGDVPRVLPPDNG
                     SLMPNLKRGRAIQPLAEPRDLDDRELFERGSYLANSLSTCGECHTQGVPGAPPGRDFA
                     LKIVTTHYLTGGSVFRVPPALQPMLHQNRTMAANLRGAKFGYFHAFGVNYLFFALIIR
                     TQIALNKIPPRPLGFPMPADKFRHLVGEDLRAIYTYGTHVTPAHISDLPRQNYARWCA
                     TNADCEEEEETCHMSADPRVGNESVGRKCRVDADCDACQTCNARVCVAPEPNSTCLPA
                     M"
     gene            6740..>7552
                     /locus_tag="VF516_28505"
     CDS             6740..>7552
                     /locus_tag="VF516_28505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111719.1"
                     /translation="MPLHTRGKSNDELVQIGYGSYLVNAAADCAGCHSSAAGFLAGGT
                     PFALDRNGHVVYARNLTPDAKTGMQLTRRQFVEAIRTGRDFHPDSHGMLVVMPWLSYR
                     WASDGDLLAIYAYLRAIPPVSNVVPPDHKAGLGLPQAIPFTGVYNEGDVRRVLPPDDG
                     SLMPNFQRGRAIQPLAEPHNLGNRKLFERGSYLANSLSDCGECHTQGVPGAPLGRDNH
                     LKLVTSHYLTGGNVFIAPPEVQLTLHQNRAMASNLRGARFGFFHEPGVDFHLF"
BASE COUNT         1249 a         2852 c         2366 g         1085 t
ORIGIN      
        1 ccgccgatgc gcaccgaggt gcgcggcacg ccgcccgggc tccgtcccct gccggatctc
       61 cgtcccctgc cggatctccg tccgacgccg gagccccgtc cgacgccgga gtcccgtccg
      121 gccgcgccca gcgcgccggc catcccgccc aacccgcggc tcgccccgca ggacatcccg
      181 ccggtccccg agcccgccga caagccggcg gacgccacca gcgagctggt cgacgcgctc
      241 caccgcggcc acgcgctgtt cgagcacggc gacatcgccg cggcgatcga ggtctacgac
      301 gcgatcgtcc gtacccaccc gtcggtcgcc gagccctggc tgttcctggg catcgcgcgc
      361 tacgcgcacg gcgacgtcga ggaggcggcc agcgcgctgc gcgccagcct gtgcctggac
      421 accacgctgt ggcccgccgg gttctacctg gcgcgcgcct acgagcggct cggccggcgc
      481 gccgatgcgc tgcagcagta cgacctgatc gccgtcgacg atctccagcc gttctcgctg
      541 ctgagcacga gcgcggtgat caacgagctg cgtgcgttcg gccacgactt ccgcaacgcg
      601 gcgcgccggc tgagcgccga gcgaacggcg accgcgcgcc ggccgttccg ttgatgggtc
      661 attccgcgtt cgaggccggt aagccgagtt acgcgcgcca cccccgggtg ttttgcacag
      721 attgcgcaac acgatcgtga tgcgatcgcg gcgtcgaagc atctcgcggt actgcgcgtt
      781 ggctgtcgtc cgtcatcgca catgtaccgc tcacaccgtt gagctccccg gggggcaaaa
      841 cgaccgagcg gtcgccagca ggtgtaagcc acctgtgcga tggaaacgct taccagccga
      901 ctcgcgatgc gggcattgtg cccccgcaac gagcgaggga caatccacat gatccgaaat
      961 accagactga ttcagctgct cgggcttttg gcatttccgg cgtcactgtc ggtcacggcg
     1021 tgcgccaccg cgatcgacga cggcgacgcc ccggccaacg agcgcgacaa ggcgctcgag
     1081 ctgctccagc gcaactccgg cgcgccggtc accatggagc tcaacgaggc cgggatcacc
     1141 cgcgtcgtcg ccatgacgcc gcggttcccg gtgccgactc acgcgaccga cgtcgcccag
     1201 gctgcgacca gcttcctcgc cagccaccac gacgtcctcc agctcgacgc ggccgacgcc
     1261 gcgagcttcg tgatcacccg ggtcgacgcc gagcccgagc gcaacatgag ccacgtcacg
     1321 ctgcagcgga cgttcaacgg catcccggtg ttccagggcg cgatcaccgt ccacatggac
     1381 ggcggcaaca gcgtgttccg cgtgctcggc gacgagttct accggatctc ggcgccgacc
     1441 aaccgcatga tgctctcgcc cgacgaggcc gcgctcgccg ccggccgcgc gctcggcgtc
     1501 tcgctgtcgc tcagcgcgag cgagaccaac ggccagagcg cggtgttcag ctcgccgggc
     1561 acgctcgacc cggtcaaggt cgatccgcag atcgtccagg tctcggccga cgacaaccgc
     1621 ctcgcgttcc gcgtgctgct gtcgtggctc gacgaccaga agcagcagca gtaccagctc
     1681 gcgctggtcg acgcccagga cggcgcgctg ctcggcagcc actcgctggt caacacgttc
     1741 cagggcaagg tgttcaacgt caacgcccag ccgaccgcca cgctggcgac cgacacccgc
     1801 accgagcagt cgttcgacgg cgatcccgcc gcgtcaccgc tcggctgggt gaccacgccg
     1861 ggcaacaaga cgatcggcaa caacgcggtc gccgcgaccg acctcgacgg caacaacacg
     1921 gtcggcacca acgagaccca gccgaccgcg gtcaacaacg cgttcgactt cccgttcgac
     1981 ccgacggtgg acgcgtccaa cttcaaggcc gccgcggtcg ccaacgcgtt cttcctggtc
     2041 aacgactacc acgaccggac ctacaagctc ggcttcaccg aggccgccgg caacttccag
     2101 accaacaaca acggcaaggg cggcgcggcg aatgacccgg tcaacgtcga ctcgcaggac
     2161 ggctcgggca ccaacaacgc caacttctcg accccgcccg acggctcgcg gccgcgcatg
     2221 cagatgttcc tgttcagcct caagaacggc accggcggcg tccgccagga cggcgacttc
     2281 gatccgagcg tgatctacca cgagaacacc cacggcctgt ccaaccgcct cgtcggcggc
     2341 ggtacgacca gctgcctcgg ggccctgcag tcgggcggca tgggcgaggg ctggggcgac
     2401 ttcatgggca gcgcgttcct caacaacccg gtggtcggcg cctacgtgac cggcaacgcg
     2461 acccgcggca tccgccagtt cccgatgaac aactcgccgt tcacctacaa cgacatcaag
     2521 aacggcacgc tcgccgaggt ccacaacgtc ggcgagctgt gggccgccac gctgtgggac
     2581 gtccgcaccg cggtcggcga cgcgatcacc cagcagctgg tcgtgtcggg catgaagctc
     2641 acgccgtgca acccgacgat gctgaacgcg cgcgacgcga tcatccaggc cgacgccaac
     2701 ctcaacgccg gcaacaaccg ctgcaagatc tggacggcgt tcgccaaccg cctgatgggc
     2761 accggcgcgt cgtcgcccaa cgccagctcg acgacgacga tcgtgacctc gaacgcggtg
     2821 cccgccgact gcgcgggcgg cggcacgggc accacggtgt tcagcgacga cttcgagacc
     2881 gacaagggct ggacggcgaa cccgaacggc accgacaccg cgaccaccgg caagtgggag
     2941 cgcggcatcc cgcaggccac gaccagcggc ggcacccagc aggtcggcac gccgaccagc
     3001 ggcacgcacg acctggtgac cggcgcgtcg gccggcgcgt ccgtgggtga caacgacatc
     3061 gacggcggca ccacgtcgat ccggtcgccg gcgatcgcga tcccggcgac cggcaccacc
     3121 acgctgtcgt tcaaggcgta cttcgcgcac ctcaacaact cgacgaccga cgacttcctc
     3181 cgggtccagg tcgtcggcac caccacgacc acggtgttcc aggtgctcgg ctcggccacg
     3241 caggtcaacg gcgcgttcgc cgcgcagtcg gcgaacatct cggcgttcgc cggccagacc
     3301 gtgcgcatcc tgatctcggc ggccgacgcc gccggcggca gcctcgtcga ggccggcgtc
     3361 gacgacatcc tgatcaccaa ccagtagtcc agaggtccga tcagcgcgtg tcccgctgat
     3421 cccgcggcgg ctgctccggc agccgccgct tgtttttttt cggcgccggc gaaccgggca
     3481 ctggcatcgc gcgcgggttt cggagtacga gcgtagccat gacgcgtgga ctcgccctcg
     3541 cctttgccat cgctctcacc ctgggcctcg cggcctgccc cgccccgcag ggcccgacgc
     3601 tcgccggcta cttcacctcg gagcccggcc tgcaggccgc cggcgtcaag cggatcccga
     3661 tccacacgcc caagggcgac ttcacggtgt ggaccaagcg gttcggcgcc aacccgcgga
     3721 tcaagctgct gctgctccac ggcggccccg gcgcgaccca cgagtacttc gaggcgctcg
     3781 agaacgacct cgccgccgcc ggcgtcgagt tcatctacta cgaccagctc ggctcggcgt
     3841 actccgacca gccgaccgac acctcgctgt ggacgctcga ccgcttcgtc gacgaggtcg
     3901 agcaggtccg ggtcgcgctc ggcctcgaca aggacaactt ctacctgctc ggccactcgt
     3961 ggggcggcat cctcgccgtc gagtacgcgc tggcccacgg cgaccacctc aagggcctga
     4021 tcatctcgaa catgatgatg agcatcccgg actacaacaa gtacgccgac accgtgctgg
     4081 ccaagcagat gccgcccgcg gtggtgtccg agatcaaggc gatcgaggcg cgcggcgagt
     4141 acgagagtcc gcgctacatg gatctcctga tcccgaactt ctaccacgcg cacatctgcc
     4201 ggctcaccga ctggcccgac gcggtcaatc gcgcgctcgg ccggatcaac cgccagatct
     4261 acgtgctgat gcagggcccc agcgagttcg gcgcatcggg cctgctcgag aagtgggacc
     4321 gcaaggccga cctgcccaag ctcacgatgc cgaccctggt gatcggcggc gcccacgaca
     4381 ccatggaccc cgatcacatg cgctgggtct cgacccaggt ccagcacggc agcttcctgc
     4441 tgtgccgcaa cggcagccac ctcgacatgt gggatgacca gcagacctac atccgcggct
     4501 tgctcaagtt cctcgacgcg gtcgaccacg gcgcccggtc ggtcacgttc tgaaccagcg
     4561 cccggcggcg cgtcggatgc gcgccacaag agcgggcgta tcccgtgtca gcgatgtgtg
     4621 cgcagccacg gaagcgcgac gccatgcgcc ggcgtcgtgc gatcaggctg tcgtcgatcg
     4681 gcatcgcgca aagtagccct gcgtgcgtcc acgcaccagc tccgatcgcg atcgcgccag
     4741 ttagcgcgac gatcgctgcg gcatgtcgag tgcagccacg tcccgccgga ggagctcatg
     4801 cgtggattcc tggttctcgt ctcgatcggt gtcatggcgg cggcgtgcac atcctccggt
     4861 gactccagtg ccacattcac tcctacttcg aacctcaccg gagcacccgg cgaggaaatc
     4921 ccacacggcg gtatgccgtg taattgcact cacgatgact acgtctgccc gcgcgccagc
     4981 cgcggattcg agatctcgcc ggtgccgatc cacacccgcg gcaagtcgtg cggcgagatc
     5041 gtgcaggcca gctacggcag ctacctcgtg aacgcgacgg ccgactgcgc gggctgccac
     5101 tcgagttctg ccggcttcct ggctggaggc aacccgttcc cgctcgatcg caagggtcat
     5161 gtcgtgtact cgcgcaacct gacgccggac ccgacgaccg ggatgcgcct cacgaaggag
     5221 cagttcgtcg aggcgctgcg caccggccgg gacttccacc cgggctcggc cgggattctg
     5281 gtggtgatgc cctggctgtt ctaccgctgg gcctcggacg gcgacctgca agcgatctac
     5341 gcctacctgc gggccatccc gccggtgaag aacttcgtgc cgcccgacgt caaggcgggc
     5401 ctcgggctgc cgcaggcgat cccgttcacc gggatctacg acgagggcga cgtgccacgc
     5461 gtcctcccgc ccgacaacgg ttcgctcatg ccgaacctca agcggggccg cgcgatccag
     5521 ccgctcgccg agccacgcga cctcgacgac cgcgagctgt tcgagcgcgg cagctacctc
     5581 gcgaactcgc tgtcgacctg cggcgagtgc cacacccagg gagtgcccgg cgcgccgccc
     5641 ggccgcgact tcgcgctcaa gatcgtcacc acgcactacc tcaccggcgg cagcgtgttc
     5701 cgcgtcccgc cggcgctcca gccgatgctc caccagaacc gcacgatggc ggccaacctg
     5761 cgcggcgcca agttcggtta cttccacgcg ttcggcgtca actacctctt tttcgcgttg
     5821 atcatccgca cccagatcgc cttgaacaag atcccgccgc gtccgctcgg cttcccgatg
     5881 ccggcggaca agttccgcca cctggtcggc gaggacctgc gcgcgatcta cacatacggg
     5941 acccacgtca cgccggccca catctccgac ctgccccggc agaactacgc gcggtggtgc
     6001 gcgaccaacg ccgactgtga agaagaggaa gagacctgcc acatgtccgc ggatccgagg
     6061 gtcggcaacg agagcgtggg ccggaaatgc cgggtcgacg ccgactgcga cgcctgccag
     6121 acctgcaacg cgagggtctg cgtcgcgccc gaacccaaca gtacgtgcct gccggccatg
     6181 tgagcctcac gacccgcgcg ccgtcgatct cggctcgtcc ccgacgacca ccacgccgac
     6241 ccaggagaac ctcgcgggcc ctgaccccgg ggacgcatcg tggcggaccg ccatgaggca
     6301 tcatccgtgt ccggatactc gctccgcgag cgggcgcccc cggcgtcatc ggcgtatgcc
     6361 cgatcacggg aacacgaggc cgtgcagcgg gtcgcgcgat caggctgtcg tcgaccagca
     6421 tcgcgcgaag tggccctgcg tgcatccgcg caccagcggt gatcgcgatt gcgccagtta
     6481 gcgcggcgat cgcggcggca tgtcgggtgc agccgcctcc cgccggagga gcttcatgcg
     6541 cggattcttc gtactcgggt tggtcggtgt cacagcggcg gcgtgcgcgt cctccgacga
     6601 ctccgatact acactcactt ctacttcaaa cctcgccggc gcacccggcg agcaaagcac
     6661 gcacggcggt ctcccgtgta atcgcgatca cgatgattac atctgccggc gcgcgatgcg
     6721 cggattcggg atctcgccag tgccgctcca cacccgcggc aagtcgaacg acgagctcgt
     6781 gcagatcggc tacggcagct acctcgtgaa cgcggcggcc gactgcgcag gttgccactc
     6841 gagcgctgcc ggcttcctgg ccggcggcac cccgttcgcg ctcgaccgca acggtcacgt
     6901 cgtgtacgcg cgcaacctga cgccggacgc gaagaccggg atgcagctca cgaggcgcca
     6961 gttcgtcgag gcgatccgca ccggccgcga tttccacccg gactcacatg ggatgctggt
     7021 cgtgatgccg tggctgtcct accgctgggc ctcggacggc gacctgctcg cgatctacgc
     7081 ctacctgcga gcgatcccgc ccgtgagcaa cgtcgtgccg cccgaccaca aggcgggcct
     7141 cgggctgccg caagcgatcc cgttcaccgg cgtctacaac gagggcgacg tacggcgcgt
     7201 cctcccgccc gacgacggct cgctcatgcc gaacttccag cgcgggcgcg cgatccagcc
     7261 gctcgccgag ccgcacaacc tcggcaatcg caagctgttc gagcgcggca gctacctcgc
     7321 gaactcgttg tccgactgcg gcgagtgcca tacccagggc gtgcccggcg cgccgctcgg
     7381 ccgcgacaac cacctcaagc tcgtcacctc gcactatctc accggcggca atgtgttcat
     7441 cgccccgccg gaagtccagc tcacactcca ccagaaccgc gcgatggcgt ccaacctgcg
     7501 cggcgcccgg ttcggtttct tccacgagcc gggcgtcgac ttccacctgt tc
//
LOCUS       DASVOR010001509         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_249469,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001509 DASVOR010000000
VERSION     DASVOR010001509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249469"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..921)
                     /locus_tag="VF516_28510"
     CDS             complement(220..921)
                     /locus_tag="VF516_28510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111720.1"
                     /translation="MRAVHAGGVAALVALASLGLGGLGCARTFRGEAVQPNPMTAPTE
                     ISPISEKITIVTGDMELEQPYAAEPSERASIANNHRYPLINQASFTVVSRDRIRFHVQ
                     IDHKWEEWANPSTWDVHLEDDQGHSWTPDSVEGARTRLISQFWDREQRTALCSNEGRD
                     PTGACIRTVGVADDGWKRRQTLGTLSVFRGNADFVFYQRDLMRAEIRKFRLVVKRPSE
                     AFVFTWRFNDTVATP"
     gene            complement(991..1713)
                     /gene="gmk"
                     /locus_tag="VF516_28515"
     CDS             complement(991..1713)
                     /gene="gmk"
                     /locus_tag="VF516_28515"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEX8111721.1"
                     /translation="MTARSDPAEAIPRRGTLLIVSSPSGAGKTTLTRRLLDEFVHPPG
                     NLVFSVSYTTRPQRPGEVEGRDYHFVTPEQFEAMVRRGEFAEHAFVFGNRYGTAQAPV
                     EAALDRGIDVIFDVDWQGGQMLHQRWPADSLKVFILPPDLDVLARRLQGRATDAPEVI
                     ERRLRKAIEELNHYDEYQHLIVNDDLDRAYAVLRAIYLTRRHGTEDRPDLPYRLGELA
                     RRVESNRTSGADAHARRLIGRL"
     gene            complement(1741..>2136)
                     /locus_tag="VF516_28520"
     CDS             complement(1741..>2136)
                     /locus_tag="VF516_28520"
                     /inference="COORDINATES: protein motif:HMM:NF019940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1732 domain-containing protein"
                     /protein_id="HEX8111722.1"
                     /translation="EPFGLVLIEALAAGVPQQIARRLTERVRRLVDDAGAGGALDPGR
                     LAQETAVLAERADITEELVRLTSHLDQARALLGAPGAVGRRLEFLVQEIGRELNTIGA
                     KATAAEIRSAIVDAKAALEKIREQVQNVE"
BASE COUNT          306 a          754 c          748 g          328 t
ORIGIN      
        1 gcctgtgcgg caaactcgta ggcgcggcgg accagcggga cgtcggcaga cgggaagtat
       61 ccggcgagct cggagatgat ctgatcgagc tggtgcatcg tatgcgggga cccatcacag
      121 ggtaccacgc ggcgcagcct cacagaaccg gccgtccggt cagctgcccc tggctgcatg
      181 gccgatcacg aaacggtccg ttcctcgctg acgctcgatt cagggcgtgg ccacggtgtc
      241 gttgaaccgc caggtgaata cgaacgcttc gctcggccgc ttgaccacga gccggaactt
      301 gcggatttcg gcccgcatca ggtcgcgctg gtagaacacg aagtcggcgt tgccgcggaa
      361 caccgagagc gtgccgagcg tctgacgccg cttccagccg tcgtcggcca caccgaccgt
      421 gcggatacac gcgccggtcg ggtcgcgccc ttcattgctg cagagcgcgg tgcgctgctc
      481 gcgatcccag aactggctga tcagccgggt gcgcgcacct tccacgctgt cgggcgtcca
      541 gctgtgaccc tgatcatcct cgaggtggac atcccaggtc gacgggttgg cccactcctc
      601 ccacttgtga tcgatctgga cgtggaaccg gatccggtcg cgcgacacca ccgtgaagct
      661 cgcctggttg atcagcgggt agcggtggtt gttggcgatc gacgcgcgct cgctgggctc
      721 ggccgcgtag ggctgctcga gctccatgtc gccggtgacg atcgtgatct tctcggagat
      781 cggcgagatc tcggtcggcg cggtcatcgg gttgggctgc accgcctcgc cgcgaaacgt
      841 ccgggcacag ccgagcccac cgaggcccag cgacgcgagc gccaccagcg cggcgacccc
      901 cccggcatga acagcgcgca taccctagcg tggcgcaacg cccccccgtc gtcaattgtc
      961 gcaacccgcg gcgcgcgccg gcgccacgtg tcagaggcgc ccgatcagac ggcgggcgtg
     1021 tgcgtcggcg ccggacgtcc ggttgctctc gacccgccgg gcgagctccc cgagccggta
     1081 tggcaggtcc ggccggtcct cggtgccgtg gcgccgcgtg aggtagatcg cgcgcagcac
     1141 cgcgtaggcg cggtcgaggt cgtcgttgac gatcaggtgc tggtactcgt cgtagtggtt
     1201 gagctcctcg atcgccttgc gcagccggcg ctcgatcacc tcgggcgcgt cggtcgcgcg
     1261 gccctgcagg cgccgggcga gcacgtcgag atccggcggc aggatgaaca ccttgagcga
     1321 gtcggccggc cagcgctggt gcagcatctg gccgccctgc cagtcgacgt cgaagatcac
     1381 atcgatgccg cggtcgagcg cggcctcgac cggcgcctgc gccgtgccgt agcggttgcc
     1441 gaacacgaac gcgtgctcgg cgaactcgcc gcgccggacc atggcctcga actgctcggg
     1501 ggtgacgaag tgatagtcgc ggccctcgac ctcgcccggc cgctgcggcc gcgtggtgta
     1561 cgacaccgag aacaccaggt tcccgggcgg gtggacaaac tcgtccagga ggcgccgggt
     1621 cagcgtggtc ttgcccgcgc cggacggaga cgacacgatc aggagcgtgc ccctccgcgg
     1681 gatggcctcg gctgggtcac tgcgcgcggt cacatcgccg attgtaacag accgggccag
     1741 ctactcgacg ttctggacct gttcccggat cttctccagc gccgccttgg cgtccacgat
     1801 cgcgctcctg atctcggcgg cggtcgcctt ggcaccgatc gtgttgagct cgcggccgat
     1861 ctcctggacc aggaactcca gccggcggcc gaccgcgccg ggagcgccga gcagcgcgcg
     1921 ggcctggtcg agatggctgg tcagccggac cagctcctcc gtgatgtcgg cgcgctcggc
     1981 gagcaccgca gtctcctgag ccagccggcc cgggtcgagc gccccgccgg ccccggcgtc
     2041 gtcgaccagc cggcgcaccc gctcggtcag ccggcgcgcg atctgctgcg gcacgccggc
     2101 ggcgagcgcc tcgatcagca cgagcccgaa cggctc
//
LOCUS       DASVOR010001510         2562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_73714,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001510 DASVOR010000000
VERSION     DASVOR010001510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73714"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VF516_28525"
     CDS             <1..341
                     /locus_tag="VF516_28525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111723.1"
                     /translation="AIGIVEIGNVGTDRAPAPPPPDPAVDSMGGLASRPTGEAAGAPA
                     SRLASLDRSTPPPRVKRGQTESDYIDSETFVGGKDDWKNALAVEDEQLVDWSAADETV
                     TNGDELGRKR"
     gene            701..1888
                     /gene="malE"
                     /locus_tag="VF516_28530"
     CDS             701..1888
                     /gene="malE"
                     /locus_tag="VF516_28530"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF007011.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HEX8111724.1"
                     /translation="MPKLTSTLRLLGVASLLVAATVRADDKGTLQIWINGDKAYNGLA
                     KVGEKFTKDTGVKVVVEHPQDAPAKFQQAASAHGGPSIFIWAHDRAGEWVAAGLIEPI
                     NPKPKFVSQFDQVGWDAFKFNGKTWGYPIAIEAIGLIYNKALVAKPPASFEEVLTLDK
                     KLAKDGKHAILWDYNNTYFTFPLLAANGGYPFGRAADGTYKAADVGVNNKGAVQGAEM
                     LAKLIESGAMPKGATYSTMEDGVNKGTIGMMISGPWAWENLKKSKIDFGVAPIPSLGG
                     KPSKAFIGVQGAMINRASNNKELAVEFIENYLLTMDGLKTLDDDVSLGVTAHKDFYKS
                     RAADPLIAATMQNIKTGLLMPSLPEMGRFWSAMASALENITGGRQKPKEALDGAAQRI
                     KAP"
     gene            2177..>2562
                     /locus_tag="VF516_28535"
     CDS             2177..>2562
                     /locus_tag="VF516_28535"
                     /inference="COORDINATES: protein motif:HMM:NF014335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX8111725.1"
                     /translation="MFSSAFSSALAVGLTLTAGRAHAQGTPADAAPQPAAVTAPLTDQ
                     QMDQVKKLVSAMPKIFEFDGYLRSGFAINAKGGDADAFQAPGAFSKYRLGNETETYGE
                     LGLTANWVNPEHNDAWFKTKVKLALVA"
BASE COUNT          472 a          841 c          860 g          389 t
ORIGIN      
        1 gcgcgatcgg gattgttgag attggcaacg tcgggaccga ccgcgccccg gcgccgccgc
       61 cgcccgatcc ggcggtcgat tccatggggg gcttggcctc tcgccccacc ggcgaggccg
      121 ctggcgcccc ggcctcgcgt ctcgcttcgc tcgaccgcag taccccgccg ccgcgggtca
      181 agcgcggcca gaccgagtcg gactacatcg actccgagac cttcgtcggc ggcaaggacg
      241 actggaagaa cgcgctcgcc gtcgaggacg agcagctcgt cgactggagc gccgccgacg
      301 agaccgtgac caacggcgac gagctcggcc gcaagcgcta ggcgcggccg cggcgccggg
      361 gcgcggtcgg tcccgacgtt gccaatctca acaatcccga tcgcgctgcg gctccccgga
      421 cacaccgtgt tgccgatcgc aacgagcagc cggctcctgc tgcaccgtcg acgatgcagc
      481 gcggcacgcg ggcacgccgg gggcatgccg gggatatgcc ggaggcatgc cggaggcatg
      541 ccggggcatg cggggcatgc ggggcatgcc ggggcatgcc gggggcatga tcaatcgcgc
      601 acgtcttgcg gcgccgtgca ggttactcgc cggtcgatct cgttgcatcc tcgcggcgat
      661 ttttctaccg tgacagcgcc gaaggaaggt acacgcactc atgcccaagc tcacaagcac
      721 gcttcgactc ctgggtgtcg cgtcgctgct cgtcgcggcg accgtacgcg ccgatgacaa
      781 aggcacgttg cagatctgga tcaacggcga caaggcctac aacgggctcg ccaaggtcgg
      841 cgagaagttc accaaggaca ccggcgtcaa ggtggtcgtc gagcaccccc aggatgcgcc
      901 ggcgaagttc cagcaggcgg cgtcggcgca cggcggcccc agcatcttca tctgggctca
      961 cgaccgcgcg ggcgagtggg tcgcggccgg tctgatcgag ccgatcaatc ccaagccgaa
     1021 gttcgtcagt cagttcgatc aggtcggctg ggatgcattc aagttcaacg gcaagacctg
     1081 gggctatccg atcgcgatcg aggcgatcgg cctgatctac aacaaggcgc tggtcgccaa
     1141 gccgccggcg agcttcgagg aggtgctgac gctcgacaag aagctcgcca aggatggcaa
     1201 gcacgcgatc ctgtgggact acaacaacac gtacttcacg ttccccctgc tggctgccaa
     1261 tggcgggtac ccgttcggcc gcgcggcgga cggcacgtac aaggccgcgg acgtcggcgt
     1321 caacaacaag ggcgcggtcc agggcgcgga gatgctcgcc aagctgatcg agtccggcgc
     1381 gatgcccaag ggcgcgacct actcgacgat ggaggacggg gtgaacaagg gcacgatcgg
     1441 catgatgatc agtggcccgt gggcgtggga gaacctcaag aagagcaaga tcgacttcgg
     1501 ggtcgcgccg atcccgtcgc tcggcggcaa gccgagcaag gcgttcatcg gcgtccaggg
     1561 cgcgatgatc aaccgcgcga gcaacaacaa ggagctcgcg gtcgagttca tcgagaacta
     1621 cctgttgacg atggacgggc tgaagacgct cgacgacgac gtgtcgctcg gcgtgaccgc
     1681 gcacaaggac ttctacaaga gccgcgcggc cgacccgctg atcgcggcga ccatgcagaa
     1741 catcaagacc gggctgctca tgccgagcct gcccgagatg ggacggttct ggtcggcgat
     1801 ggcatcggcg ctggagaaca tcacgggggg acggcagaag cccaaggagg cgctcgacgg
     1861 cgcggcgcag cgtatcaagg caccgtagat ctcgatcttt ccatttcaga tcccggccgg
     1921 tttgtttgct ggatcgccgg ccgcgcatga ggcaatcctc accggacttg tcgtcgggtg
     1981 aggattgctt atttgagata cccgatcgcc gatctcggcg gcgtggtgag acgtcaagat
     2041 cctggcgcgc aaacttcaca ctgtgcgctc atgcccttgt gtggcgtgat cgtgtgtgct
     2101 cccatcgcgc tcgccagtcc catccactgc ggaaacggcg gacgatatga gcacaaacaa
     2161 gaaccgtatc cttcgagtgt tctcctcggc attctcttca gcactggcgg tcgggctcac
     2221 gctgaccgcc ggtcgcgcgc atgcgcaggg cacgccggcc gacgctgcgc cccagccggc
     2281 cgccgtgacc gctccgctca cggaccagca gatggaccag gtcaagaagc tggtctcggc
     2341 gatgccgaag atcttcgagt tcgacggcta cctgcgctcc gggttcgcca tcaacgccaa
     2401 gggcggtgac gccgacgcgt tccaggcgcc cggcgcgttc agcaagtacc gcctcggcaa
     2461 cgagaccgag acctacggcg agctcgggct gaccgccaac tgggtcaacc ccgagcacaa
     2521 cgacgcctgg ttcaagacca aggtcaagct cgcgctggtc gc
//
LOCUS       DASVOR010001511         2150 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_571294,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001511 DASVOR010000000
VERSION     DASVOR010001511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571294"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1380
                     /locus_tag="VF516_28540"
     CDS             127..1380
                     /locus_tag="VF516_28540"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8111726.1"
                     /translation="MLDPNHAADFVFDPDAPAFVSDPFPTYAWLREHAPVYRWDQGRA
                     FLVSCHADVAQLLADRRFSINGRDWIHAPPVPNDGPLAEFFAFLATSLTAQPSADHLR
                     LRRLVNPAVTPRAVARMRERVQRVVDTTLNEAMLNAAIRGESLDVRRDFAEWLPLRVI
                     ADIFDIPAAHEATFRAFSAALIIAVNPWIVPEQLLRMASVAIEGMGILLEIIDERRRH
                     LGDDLLSDLIRVEEAGDRLTSQELLRLIASLVIAGADTTVHALCFAVLDLLRHPEAWR
                     AVVADRSLLRNAIEESLRHQPFGKFGALPRYALEDAELRGTRIPKGEMAVPVISATGR
                     DPSAYPDPDVYDIHRKLSSTLAFGIGPHVCVGAALARLELEVAIGTLIERFPSMTLVD
                     EAPVFRPNPQMRDMESLRVALRGEA"
     gene            complement(1363..1755)
                     /locus_tag="VF516_28545"
     CDS             complement(1363..1755)
                     /locus_tag="VF516_28545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111727.1"
                     /translation="MTSSPDAATAGPAAAIDPLPPALSSMWRLCKLGYRREPRLMLVA
                     AEQKLVVDRRGAVARVEGGRVDPSSVRRHGERAGRITEQREDRDRIAPERAAQVVRVE
                     HPDVWIPPTSGTSTSRLIVLSKLSRFAA"
     gene            complement(1752..>2150)
                     /locus_tag="VF516_28550"
     CDS             complement(1752..>2150)
                     /locus_tag="VF516_28550"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111728.1"
                     /translation="DTILARGYDGGTDLSGGQWQRVALARALAAVQLGAGMVLLDEPT
                     AQLDVRGEAEIFDRILAATRHATTILISHRFSTVRHADRICVLEHGRVVELGSLDELI
                     ARGGRYRTMFDLQAQRFTDPDEEGRRYDEL"
BASE COUNT          302 a          792 c          738 g          318 t
ORIGIN      
        1 cgccgcgatc ccctcgagta gccgtggggc attctgcggc gtatcgctcc cgcacgccgg
       61 cggtacactc gccggcccga gcgacgggtg attggagccc cggacgtctt ggtgaggagg
      121 tgcccgatgc tcgacccgaa ccacgccgcc gacttcgtct tcgatcccga cgcccccgcg
      181 ttcgtgagtg acccgttccc gacctacgcg tggctgcgcg agcacgcgcc ggtgtaccgc
      241 tgggaccagg gccgcgcgtt cctggtcagc tgccacgccg acgtcgccca gctcctcgcg
      301 gaccgccgct tcagcatcaa cggacgcgac tggatccacg cgccgcccgt gccgaacgac
      361 ggccccctcg ccgagttctt cgcgttcctg gcgaccagcc tgaccgctca gccctcggcc
      421 gaccacctcc gcctccgcag gctcgtcaac ccggcggtca ccccgcgcgc ggtggcccgc
      481 atgcgcgagc gggtgcagcg cgtcgtcgac accacgctga acgaggcgat gctgaacgcg
      541 gcgatccggg gcgagtcgct cgacgttcgc cgcgacttcg ccgagtggct gccgctccgg
      601 gtgatcgccg acatcttcga catccccgcc gcgcacgagg ccaccttccg ggcgttctcc
      661 gccgcgctga tcatcgccgt gaacccctgg atcgtccccg agcagctgct ccggatggcg
      721 agcgtcgcga tcgagggcat gggcatcttg ctcgagatca tcgacgagcg caggaggcac
      781 ctcggtgacg acctgctcag cgacctgatc cgggtggagg aggccggcga tcgcctgacc
      841 agccaggagc tcctcaggct catcgcttcg ctggtcatcg ccggcgcgga caccaccgtg
      901 catgccctct gcttcgccgt gctcgacctg ttgcggcacc ccgaggcgtg gagggccgtc
      961 gtcgcggacc gttcgctcct gcgcaacgcg atcgaggagt cgctgcggca ccagccgttc
     1021 ggcaagttcg gcgcgcttcc ccgctacgca ctcgaggacg cggagctccg gggcacacgg
     1081 atcccgaagg gcgagatggc ggtgcccgtc atctccgcca cgggccgcga cccgagcgcg
     1141 tacccggacc cggacgtcta cgatatccac cgcaagctgt cctcgacgct ggcgttcggc
     1201 atcggccccc acgtctgcgt gggcgcggcg ctggcccggc tcgagctcga ggtcgcgatc
     1261 ggtacgctga tcgagcgctt cccgtcgatg acgctggtcg acgaggcgcc ggtgttccgg
     1321 cccaacccgc agatgcgcga catggaatcg ctgcgcgtcg cgctacgcgg cgaagcgtga
     1381 cagtttcgac aggacgatga gccgcgaggt ggacgtgccc gaggtgggag gaatccagac
     1441 gtctgggtgt tcgacgcgga ccacctgggc agctcgctcg ggggcgatcc gatcacgatc
     1501 ctcacgctgt tcagtgatac gccccgcgcg ctcgccgtga cgccggacgg agctcgggtc
     1561 tacacggccg ccttccactc gggcaaccgc accacgtcga tcaaccacaa gcttctgctc
     1621 ggccgcgacc agcatcagcc gcggctcgcg gcggtagccg agcttgcaca gccgccacat
     1681 cgacgacagc gccgggggca gcgggtcgat cgccgctgcc ggcccggcgg tcgcggcgtc
     1741 aggcgaggac gtcatagctc gtcatacctc cggccctcct cgtcgggatc ggtgaaccgc
     1801 tgggcctgca ggtcgaacat cgtgcggtag cggccgccgc gcgcgatcag ctcgtcaagg
     1861 ctgccgagct cgacaacccg gccgtgctcg agcacgcaga tccggtccgc gtggcgcacg
     1921 gtcgagaacc ggtgcgagat caggatcgtg gtggcgtgcc gcgtggcggc gaggatgcgg
     1981 tcgaagatct cggcctcgcc gcgcacgtcg agctgcgcgg tgggctcgtc gagcagcacc
     2041 atgccggcgc ccagctgcac ggccgccagc gcgcgggcga gcgcgacgcg ttgccactgg
     2101 ccgccggaca ggtcggtgcc gccgtcgtag ccgcgggcca ggatggtgtc
//
LOCUS       DASVOR010001512         3426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_16287,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001512 DASVOR010000000
VERSION     DASVOR010001512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16287"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF516_28555"
     CDS             complement(<1..251)
                     /locus_tag="VF516_28555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111729.1"
                     /translation="MIRRHNVNSAVFQAAVKDASTPLDPSILTHVTRLQEMDADRQGM
                     VMAFLAGYHPRGGIEFMEIMGKELEIPQHLDHPTFDERVA"
     gene            complement(248..1642)
                     /locus_tag="VF516_28560"
     CDS             complement(248..1642)
                     /locus_tag="VF516_28560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEX8111730.1"
                     /translation="MADPDLQGRGQSNAEPTIAADPNQPDHLVASANDNVRGDQVCGS
                     YWSSDGGRTWHDSALPSNFVRGTRFGADRQYFQVCGDTSVAWDSKGNAYELGLMFMRG
                     PPTSNNPDFSSGIYVFRSTGNHGASWDFPGHPVIELADFTGTVVLDKPYMTVDNWPSS
                     PFRDRVYATWSFFGSNGTVYIYGAYSSDYGQTFSKPVLVSTNSKLCGNTYGLPIPHGA
                     CAARRCATFSSRSRANRSTPIRITGPASCRPATTRRSGRLRSRCQAKRYPMKSHTFVR
                     AAALAVATAAALALPAGPAAAAPRKPAPAAPARLDKKHLEIERHVWTAQFYLRKAGDV
                     AGAAREYKAVLALDPQNVDASLALASLYAREGYRAAVGLMEQALGGVDDAAPEKPVIE
                     DIVHRLTEASAVKQQFRVSMLATSIVNAFALPDGHVYVTRGMIDLMKKKQPSARSTPT
                     TTRSATSSATSCST"
     gene            complement(2118..2888)
                     /locus_tag="VF516_28565"
     CDS             complement(2118..2888)
                     /locus_tag="VF516_28565"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HEX8111731.1"
                     /translation="MNEADPALPRERQDRILERLRRHGRVIAADLAVEFRVSEDSIRR
                     DLRELAARSLCKRVYGGALLATTTPPLAERRRKDVERKRALAAVAATLVSPRQILFLD
                     AGSTNSAIASALPEGHDLTVVTNAPDVALALIGRSGFEVLTIGGRIDPRIGGAIGAMA
                     LRQIQGIRADLCFPGVCAADAQTGLWTLDAEEVVLKRTMIEASGETVLVATREKLIRA
                     ATHHTAPPSSITHLVTERDTDPAILAAWRAAGTSVHVV"
     gene            complement(2885..>3426)
                     /locus_tag="VF516_28570"
     CDS             complement(2885..>3426)
                     /locus_tag="VF516_28570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111732.1"
                     /translation="ATAVRLGLLCLVLFLAEGAMLDWSAVLLRSERGVALSDAGLGYA
                     AFSIAMAIGRLLGDRVTAALGPTRIVRYGSLVAAAGFALAGALPWATSSMAGFVLVGL
                     GASNIVPVLFSAAGRLPGTAPGVAIATVTTFGYTGLLAGPALIGAVAHALSLPLALGG
                     LTALLITVSAGASIVRRSW"
BASE COUNT          521 a         1116 c         1247 g          542 t
ORIGIN      
        1 gcgacgcgct cgtcgaacgt cgggtgatcg aggtgctgcg ggatctcgag ctccttgccc
       61 atgatctcca tgaactcgat cccgccgcgc gggtggtagc cggcgaggaa cgccatcacc
      121 atgccctggc ggtcggcgtc catctcctgc agccgggtga cgtgggtgag gatcgacgga
      181 tcgagcgggg tcgacgcgtc cttgaccgcg gcctggaaca ccgcggagtt gacgttgtgg
      241 cggcggatca cgtgctgcag ctcgtggccg aggatgtggc cgagcgcgtc gttgtcggcg
      301 tcgatcgggc gctgggctgc ttcttcttca tcagatcgat catcccgcgc gtgacgtaga
      361 cgtggccgtc gggcagcgcg aacgcgttga cgatgctggt ggcgagcatc gagacccgga
      421 actgctgctt gaccgcgctg gcctcggtca gccggtggac gatgtcctcg atcaccggct
      481 tctcgggcgc ggcgtcgtcg accccgccga gcgcctgctc catcaggccg accgcggcgc
      541 ggtagccctc gcgcgcgtag agcgacgcga gcgcgaggct ggcatcgacg ttctgcggat
      601 cgagcgcgag caccgccttg tactcgcggg cggcgccggc gacgtcgccg gccttgcgca
      661 ggtagaactg cgcggtccac acgtggcgct cgatctccag atgcttcttg tcgagcctgg
      721 cgggcgcggc cggcgcgggc ttcctcggcg cggcagccgc ggggccggcc ggcagggcga
      781 gcgccgcggc cgtggcgacg gcgagggcag cggcgcgtac gaacgtgtgt gacttcatcg
      841 gatacctctt ggcctggcac ctgctacgga gtcggcccga gcggcgtgtc gtcgccggcc
      901 ggcaggaagc tggcccagta atacggatgg gcgtacttcg gtttgcccgg gatctcgagc
      961 tgaacgtcgc gcagcgccga gctgcacagg cgccgtgcgg gattggcagg ccatacgtgt
     1021 tgccacacag cttgctgttg gtgctgacca gaacaggctt gctgaaggtt tgaccgtaat
     1081 cggacgagta ggcaccgtag atgtacaccg tgccattcga gccgaagaag ctccacgtcg
     1141 cgtagacgcg gtcccggaac gggctggacg gccagttgtc gacggtcatg tagggcttgt
     1201 ccagcacgac ggtgccggtg aagtcagcca gttcgatgac gggatggccg gggaagtccc
     1261 acgaggcgcc gtggttgccg gtcgaccgga acacgtagat gccgctcgag aagtctgggt
     1321 tgttggaggt aggggggccg cgcatgaaca tcaggcccag ctcgtaggcg ttacccttgc
     1381 tgtcccaggc cacggaggta tcaccacata cctgaaagta ctgacggtct gcaccgaacc
     1441 tggtgccgcg gacgaagttg ctcggcagcg cgctatcgtg ccacgtccgc ccgccgtcgc
     1501 tgctccagta gcttccacat acctgatctc cacgcacgtt gtcgttggcg ctggccacga
     1561 ggtggtcggg ctgattgggg tccgcggcga tcgtcggctc ggcgttcgac tgacctcggc
     1621 cctggaggtc cggatcagcc acgttcaggc agttctgatt caccttgatg ttggagtccc
     1681 ggcggatcgg gcagtcagct ttgccgcgag ggtagtaatt cctgagtgtc cgcgcgctgg
     1741 attgaacgga atatccaggt cgtatgacag gaatcccgac aggatgcgct tctgtatcct
     1801 gttcatgtgc ctgatggcgg gttgttgcgc actgggcgtt gggctggcgg acgtgtacgc
     1861 cagcgatcca ccaatgacag agactaccac caccgccaga atgcgaagtc tcatgacctt
     1921 ccccttcctt cccttcctgc cggacatttc caggagattc ggtggatgga tccacgctgg
     1981 tgcgatatgt cgagcgcgat ggactcgagc ctggtgcagc gctcggctcc ctgggatgac
     2041 cgagagcagc gatgctacag gtcaggatga cctcaacacg actgctcgtc gacacacatc
     2101 tgtgtgagac gatctaacta gacgacatgc acgctggtgc cggcagcgcg ccaggcggcg
     2161 aggatcgccg gatcggtgtc gcgctcggtg accagatggg tgatgctgct cggcggcgcg
     2221 gtgtgatggg tcgcggcgcg gatcagcttc tcgcgggtcg cgaccagcac ggtctcgccg
     2281 ctggcctcga tcatcgtccg cttgagcacg acctcctcgg cgtcgagcgt ccacagcccg
     2341 gtctgggcgt cggccgcgca cacgccgggg aagcacaggt cggcgcggat gccctggatc
     2401 tggcgcagcg ccatcgcgcc gatcgcgccg ccgatcctgg gatcgatccg gccgccgatc
     2461 gtgagcacct cgaagcccga ccggccgatc agcgcgagcg cgacgtcggg cgcgttggtg
     2521 accacggtca ggtcgtggcc ctcggggagc gcgctggcga tcgccgagtt ggtcgagccg
     2581 gcgtcgagga acaggatctg gcgcgggctg accagcgtcg cggcgacggc ggccagggcg
     2641 cgcttgcgct cgacgtcctt gcggcggcgc tcggcgaggg gcggcgtggt ggtcgcgagc
     2701 agcgcgccgc cgtagacccg cttgcacagg ctgcgcgcgg cgagctcgcg caggtcgcgg
     2761 cggatcgagt cctccgagac ccggaactcg accgcgagat cggcggcgat gacgcggccg
     2821 tggcggcgca ggcgctcgag gatccggtcc tggcgctcac gcggcagcgc cgggtcggcc
     2881 tcgttcacca cgagcgcctc acgatcgacg cgccggccga caccgtgatc agcagggcgg
     2941 tgagcccgcc gagcgcgagc ggcaggctga gcgcatgggc gacggcgccg atcagcgcgg
     3001 gcccggcgag caggccggtg tagccgaacg tcgtgaccgt ggcgatcgcg acgccgggcg
     3061 cggttcccgg aagccggccg gcggcgctga acagcaccgg cacgatgttg gacgcgccga
     3121 gaccgacgag cacgaacccc gccatcgagc tggtcgccca cggcagcgcg ccggcgagcg
     3181 cgaacccggc ggccgcgacc aggctgccgt agcgcacgat gcgcgtcggc ccgagcgcgg
     3241 cggtcacccg gtcgccgagc agccggccga tcgccatcgc gatcgagaac gccgcgtagc
     3301 ccaggccggc gtcggacagc gcgacgccgc gctcggagcg cagcagcacc gcgctccagt
     3361 cgagcatcgc gccctcggcg aggaacagca ccaggcacaa caggccgagc cgcaccgcgg
     3421 tggccg
//
LOCUS       DASVOR010001513         2207 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_394685,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001513 DASVOR010000000
VERSION     DASVOR010001513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394685"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="VF516_28575"
     CDS             complement(<1..273)
                     /locus_tag="VF516_28575"
                     /inference="COORDINATES: protein motif:HMM:NF013543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEX8111733.1"
                     /translation="MAVTTVVVVTVLVALAFDVINGFHDAANSIATIVATRLMSPRKA
                     VAWAAFFNFAALFIFHQGVARTVSGLVKVDARDPAFVYVVLAGLIGA"
     gene            complement(282..908)
                     /locus_tag="VF516_28580"
     CDS             complement(282..908)
                     /locus_tag="VF516_28580"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEX8111734.1"
                     /translation="MLRKLLPRETSFFDFFEQHAELTLDGATEFLSLVNSGVNIEAKA
                     RRIKEIEHETDVITHRCVEALHKTVITPMDSTQIHRLITRMDDVMDAIEAAAERMALY
                     KLRQMTPEVQAMADVLKSATQVLLDACRLLRNVRDPAAILKKCVDINRLENEADAILR
                     CAVARLFDQEEDPVMIIKWKEIYESLETAADRCEDVANIIENVILDLT"
     gene            complement(942..2039)
                     /locus_tag="VF516_28585"
     CDS             complement(942..2039)
                     /locus_tag="VF516_28585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111735.1"
                     /translation="MRGPLIKKAWVLVPSLLALIAMVPTYSWTARYRMPGEARVRQLT
                     FDSQGPDQVINEFAVASPDGKYFLIQHSEDPKLVTQPDGTKVMDFKETSNWDIYRYNV
                     DGTGRLQLTSDPASEDQPTWSPDGKTIVYRRLNGKQFDLYLMDADGSNKRELLIDPAH
                     DEKTPAFSRDGKKVVFFSDRDGIKWNLYTIDIATRKVERLTHDKVEDKHPQFTPDGHV
                     VFHSSRDSTHVTLEGGEEFDIMNLWSLDPATGTLAELTSSGSFSSAYGMRDNRHAWIS
                     PDGKYVAFHSNVIDADRKRPGIYRKRHRELCIATIDGSRRVFLTMGDHRNFKHPSWAA
                     DGKGLFFVFKEKGQAWNAGFMDVSAALERLR"
BASE COUNT          349 a          717 c          721 g          420 t
ORIGIN      
        1 cgcgccgatc aggccggcga gcacgacgta cacgaacgcc ggatcgcgag cgtcgacctt
       61 gaccaggccg gacaccgtcc gggccacgcc ctggtggaag atgaacagcg cggcgaagtt
      121 gaagaacgcg gcccatgcca cggccttgcg cggggacatc agccgggtcg ccacgatcgt
      181 cgcgatgctg ttggcggcgt cgtggaagcc gttgatgaca tcgaacgcca gcgcgaccag
      241 caccgtgacg acgacgacag tggtgacagc catcgtgcac ctcaggtcag gtcgaggatg
      301 acgttctcga tgatgttggc cacgtcctcg cagcgatccg ccgcggtctc gaggctctcg
      361 tagatctcct tccacttgat gatcatcacc gggtcctcct cctgatcgaa cagccgcgcg
      421 accgcgcagc gcaggatcgc gtcggcctcg ttctccagcc ggttgatgtc gacgcacttc
      481 ttcaggatcg cggcggggtc acggacgttg cgcagcagcc ggcacgcgtc gagcaggacc
      541 tgggtcgcgg acttgagcac gtccgccatc gcctggacct cgggcgtcat ctggcgcagc
      601 ttgtacagcg ccatgcgctc ggcggcggcc tcgatcgcgt ccatcacgtc gtccatccgc
      661 gtgatcagcc ggtggatctg cgtcgagtcc atcggcgtga tcacggtctt gtgcagcgcc
      721 tcgacgcagc ggtgcgtgat cacgtcggtc tcgtgctcga tctccttgat ccggcgcgcc
      781 ttggcctcga tgttcactcc cgagttgacc agcgacagga actccgtcgc gccatcgagc
      841 gtgagctcgg cgtgctgctc gaagaagtcg aagaacgaag tttccctggg cagcagcttg
      901 cgcagcatgc gtgcactcct tgtgtcgtcg tgggttgcga ttcagcgcag gcgttcgagc
      961 gccgcgctga cgtccatgaa ccccgcattc cacgcctgac ccttctcctt gaacacgaag
     1021 aacaggccct tgccgtcggc cgcccagctc gggtgcttga agttgcggtg atcgcccatg
     1081 gtgaggaaca cgcggcgcga gccgtcgatc gtcgcgatgc acagctcgcg atgccgcttg
     1141 cgatagatcc cggggcgctt gcgatcggca tcgatgacgt tgctgtggaa cgcgacgtac
     1201 ttgccgtccg gggagatcca ggcgtgccgg ttatcgcgca tcccgtaggc ggagctgaac
     1261 gaacccgacg acgtcagctc ggcgagcgtc ccggtggctg gatcgagcga ccacaggttc
     1321 atgatgtcga actcctcgcc gccctccagc gtcacgtggg tcgagtcgcg gctggagtgg
     1381 aacaccacgt gcccgtcggg cgtgaactgc gggtgcttgt cctcgacctt gtcgtgggtc
     1441 aggcgctcga ccttgcgggt cgcgatgtcg atcgtgtaga ggttccactt gatgccatcg
     1501 cgatcggaga agaacacgac cttcttgcca tcgcgcgaga acgccggggt cttctcgtcg
     1561 tgcgcagggt cgatcagcag ctcgcgcttg ttgctgccgt cggcgtccat caggtagaga
     1621 tcgaactgct tgccgttgag cctgcggtag acgatggtct tgccgtccgg cgaccaggtc
     1681 ggctggtcct ccgacgcggg atcgctggtg agctggaggc gccccgtacc gtcgacgttg
     1741 tagcggtaga tgtcccagtt cgaggtctcc ttgaagtcca tcaccttggt gccgtcgggc
     1801 tgggtgacca gcttcgggtc ctcggagtgc tggatcagga agtacttgcc gtcgggcgag
     1861 gcgaccgcga actcgttgat cacctggtcg ggtccctggc tgtcgaacgt cagctggcga
     1921 acgcgcgcct cgccgggcat ccggtagcgc gcggtccagg agtaggtcgg caccatcgcg
     1981 atcagcgcca gcagcgacgg caccaggacc cacgccttct tgatcagggg tcccctcaca
     2041 ggtcgacttc ttgctgaccc agcacgaggc tgaccttctg gttctcgttc acgttacgca
     2101 gcgtctcgat caaccggcgc ggctcggtgc tgcgcttgag caccacgctg tacaccagct
     2161 cctgcaggct gccgccgccc accgactcca tcgagatcag gctgctg
//
LOCUS       DASVOR010001514         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_483177,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001514 DASVOR010000000
VERSION     DASVOR010001514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483177"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VF516_28590"
     CDS             <1..485
                     /locus_tag="VF516_28590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111736.1"
                     /translation="SGSLGIMFGGGAGTGRYARSLGWGYYQFGAQAAWQPMTTEQRIG
                     WSLKGSFLFGTMYDADSAQIDLYLRTLQMDFLAGLRIRPGESRLRYLALRGGVELFRT
                     NEPVKPDGGRAFVGPVADAAIEQYAFGAVLFNADVRYGLLGSGPAEIAILVGASISVP
                     "
     gene            491..1012
                     /locus_tag="VF516_28595"
     CDS             491..1012
                     /locus_tag="VF516_28595"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8111737.1"
                     /translation="MPALVTHRLRLVPITLEAIEAVLDHDQPRAEAIVGARFPPGWPN
                     DPLLDVGFPYSREAIRADPDVRLWGDSLVILRDEPRVVGSVVFHGHPSDGVAEIGYAI
                     EERARGIGLATEATRACAEWALAQAGIAALRATTFPWHAASLGVIRNLGMRQIATRDH
                     DTLGELLVFERRR"
     gene            complement(1185..>2362)
                     /locus_tag="VF516_28600"
     CDS             complement(1185..>2362)
                     /locus_tag="VF516_28600"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HEX8111738.1"
                     /translation="LCGARLALLGSGLLASGAPVGAAPAADSPPAASPTPDIIGGTTT
                     TVGQYPTVVGLEIGSFLCTGTLIAPTWVLTAGHCVDPAVVMLPSQDAVTKGTTVHFNT
                     VDIVSDPGTPVMASATFKDPLFNPDPARLGSNDLGLIQLATPVTDVTPSPINLSAALA
                     PVGTVATIVGYGLTSQPSGAGTTGIEFELKNRISIPCSSKSIGSDINLLCFSQTDNKG
                     TCSGDSGGPAFATINGRQTLVGVTSFGDMQCASYGADTRIDIEQPFLVQHVPELIGCL
                     TDKDCPEHRVCFAHSCIAQPFGPNGIGTVCNTPADCDSSICAVSSQDGKRCSLACSPS
                     DPGTCPDSFECLQQTTSVGACWPVPGGGCCDAGPGSSPATALVGLGAVALWLRRRRR"
BASE COUNT          315 a          853 c          848 g          346 t
ORIGIN      
        1 cgtcgggcag cctcggcatc atgttcggcg gcggcgccgg caccggccgg tacgcgcgct
       61 cgctcggctg gggctactac cagttcggcg cccaggccgc gtggcagccg atgaccaccg
      121 agcagcggat cggctggtcg ctcaagggct cgttcctgtt cggcacgatg tacgacgccg
      181 actccgcgca gatcgacctc tacctccgca ccctgcagat ggacttcctc gcggggctgc
      241 ggatccgccc cggcgagagc cggctgcgct acctcgcgct gcgcggcggc gtcgagctgt
      301 tccgcaccaa cgagccggtc aagcccgacg gcggccgcgc gttcgtcggc ccggtcgccg
      361 acgccgcgat cgagcagtac gcgttcggcg ccgtgctgtt caacgccgat gtccgctacg
      421 gcctgctcgg ctcggggccc gccgagatcg ccatcctggt cggtgcctcg atcagcgtgc
      481 cgtagccgcc atgcccgcgc tggtcaccca ccggctgcgg ctcgtgccga tcaccctcga
      541 ggcgatcgag gcggtgctcg atcacgacca gccgcgcgcc gaggcgatcg tcggggcgcg
      601 cttcccgccg ggctggccca acgatccgct gctcgacgtc ggcttcccgt actcgcgcga
      661 ggcgatccgc gccgatcccg acgtccggct gtggggcgac tcgctggtga tcctgcgcga
      721 cgagccgcgc gtcgtcggct cggtcgtgtt ccacggccac ccgtccgacg gcgtcgccga
      781 gatcggctac gcgatcgagg agcgcgcgcg cggcatcggc ctggccaccg aggccacccg
      841 cgcatgcgcc gagtgggcgc tcgctcaggc cggcatcgcc gcgctgcgcg ccaccacctt
      901 cccgtggcac gccgcctcgc tcggcgtgat ccgcaacctc ggcatgcgcc agatcgccac
      961 ccgcgatcac gacacgctcg gcgagctcct ggtgttcgag cgccggcgat gaggatccca
     1021 ggtgagcagc tctcggccgc ggccaagcca tcaaccagcc cacctcgaac ggcacgtcag
     1081 ccatccatga acgctgcctc gtacgtgcgt cgtgccaagg cagcgatgac gatcgcggtt
     1141 cattttcgcg ataatcctcg ccgatcgagc gccgccggca gtgttcagcg ccgccgccgg
     1201 cgcagccaga gcgcgaccgc gccgaggccg accagcgcgg tggccgggct gctgcccggg
     1261 ccggcgtcgc agcagccgcc gccgggcacc ggccagcacg cgccgacgct ggtggtctgc
     1321 tggaggcact cgaagctgtc cggacacgtg cccggatcgg acgggctgca ggccagcgag
     1381 catcgcttgc cgtcctggct gctgaccgcg cagatcgagg agtcgcagtc ggcgggagtg
     1441 ttgcacaccg tgccgatgcc gttggggccg aacggctggg cgatgcagct gtgcgcgaag
     1501 cacacccggt gctccgggca atccttgtcg gtcaggcaac cgatcagctc ggggacgtgc
     1561 tggaccagga acggctgctc gatgtcgatc cgggtgtcgg cgccgtagct ggcgcactgc
     1621 atgtcgccga acgaggtcac gccgaccagg gtctgcctgc cgttgatggt cgcgaacgcc
     1681 ggcccgcccg agtcgccgga gcaggtgccc ttgttgtcgg tctgcgagaa gcacagcagg
     1741 ttgatgtcgc tgccgatgct cttgctggag cacgggatcg agatccggtt cttgagctcg
     1801 aactcgatgc cggtggtgcc ggccccgctg ggctgcgagg tgagcccgta gccgacgatg
     1861 gtcgcgacgg tgccgaccgg ggccagcgcc gcgctgaggt tgatcggcga cggggtcacg
     1921 tcggtgaccg gcgtcgcgag ctggatcagg cccaggtcgt tggagcccag gcgggccggg
     1981 tccgggttga acagcgggtc cttgaacgtc gcgctcgcca tcaccggcgt gcccgggtcg
     2041 ctgacgatgt cgaccgtgtt gaagtggacc gtggtgccct tggtcaccgc gtcctgcgac
     2101 ggcagcatga ccacggcggg atcgacgcag tgcccggcgg tcagcaccca ggtcggagcg
     2161 atcagcgtgc cggtgcacag gaagctgccg atctccagcc cgaccaccgt ggggtactgg
     2221 ccgaccgtgg tggtcgtgcc gccgatgatg tcgggcgtcg gagaggccgc cggcggggag
     2281 tccgcggcgg gcgcggcccc gaccggcgcc cccgacgcca gcagcccgct cccgagcagc
     2341 gcgagccgcg ctccgcaaag cc
//
LOCUS       DASVOR010001515         3455 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_483185,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001515 DASVOR010000000
VERSION     DASVOR010001515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483185"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VF516_28605"
     CDS             <1..1040
                     /locus_tag="VF516_28605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111739.1"
                     /translation="LCRADASPEQARAEVTEMVAALRRRGLVSTRSNRQTDRGVRALH
                     TALGLARVPAWDQVPALLAALALGFPAESEAAQLVSTEDALAAAVLCERLEIPGAATT
                     ESMCDALIAEALGMPPGTTLDQIRGHVLARRAERAGIAATDINARRQPADLAAHLAGI
                     AVNARPTEESPLAKALARRWVCEATEPACLPAGAPAHAASAFPDHHDERSMSVPVTPP
                     APAAPRTDDAARRPPPPPETVLEVVRETLPRVGADGRFGAENVFVSAIWHRIADDRRL
                     VDLSFDRFKRWLVNANRDGWLVLARADLVGAMDPRQVAESEIEDRGATFHFVLDQQAE
                     ALAFHQGSHAR"
     gene            1030..>3455
                     /locus_tag="VF516_28610"
     CDS             1030..>3455
                     /locus_tag="VF516_28610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111740.1"
                     /translation="MLDNTRLRAELTHIHHALSAVAASPRGKGMVDDLRGAIGGTTVA
                     ILWQALFADCLRLAYSAVAADGEIGDDEIDALYELLFSAARHYARVIPARYSDFTAVD
                     HELARAFLERYAADSGPFGRGAAQHWPGLTLCRRAAELGDDHALERYERTMTWLIGAA
                     CQVGGVTEADPRWRGRVDELDDLRRTLARDAKVEAPAADLRVEAFLAPTRVFTAVEQA
                     SSIFENDPFDVETIHREARAVFEQMVERATTPSQITDRGRMLLVLGASGAGKTHLLRG
                     FRRHVHEYGRGFVVYAQLQSETDDYGRYLLQHLVDSLARPYAGPSGERTGLHELASGL
                     PRLVGQPLKSRIECLADDTWDSPASLNDYVNQLTDDLLREADLASFDPDLLRIMLYAL
                     HPDQRTTSRVYKYLRCEDMGDYDRRWIGDVVPRTGDDHPYWMIRELGRLAAVTQGAAL
                     VLMVDQADLAGFEAASGRMFRRAIDTLYRIVSEIPSAIAVVACLSNLYEKVRAELSRP
                     ALDRLEKDPPIAQLQINRSYAEIEAIVARRLSWLFAERGTVHRPDEPVYPIPEAQLKQ
                     LENRRTRDILEWCHQLQAQCAAAGRIVELDDGGAISDPPPPPRSDADLDHIAAAWNDA
                     MHARGIDVPDEEDEILAVIGAAARVCADEVGLTLTAPPRRNGALRVQLSAAAESADLA
                     IAVTNRGYQRGSFAGQIEALRKTADKATPVAVRTLKFPHGETSDQAIAQLLKAGGRRV
                     YVDASALRTLVAFQRFQPAFPPDRVAAWRQRDRPISTLPAMIDIFDLERLRSSPAAAP
                     PRDPPPSSTPA"
BASE COUNT          491 a         1280 c         1215 g          469 t
ORIGIN      
        1 agctgtgccg ggccgacgca tcgcccgagc aggcccgtgc ggaggtcacc gagatggtgg
       61 ccgcgctgcg gcgacgcggc ctggtcagca cgcgaagcaa ccgccagacc gaccgggggg
      121 tccgcgcgct ccacaccgcg ctcggcctcg cgcgcgtccc cgcgtgggac caggtccctg
      181 cgctgctggc ggcgctcgcg ctcggcttcc cggccgagtc ggaggccgcc cagctggtgt
      241 ccaccgagga cgcgctggcg gcggccgtgc tgtgcgagcg gctggagatc cccggcgcgg
      301 cgacgaccga gtcgatgtgc gacgcgctga tcgccgaggc actggggatg ccacccggca
      361 cgacgctgga ccagatccgc ggccacgtgc tcgcgcggcg cgccgagcgc gccgggatcg
      421 cggcgaccga catcaacgca cggcggcagc cggccgatct ggcggcgcac ctggcaggca
      481 tcgcggtgaa cgcgcgcccg accgaggaat caccgctcgc caaggcgctc gcccgccgct
      541 gggtgtgcga ggcgaccgag cctgcgtgcc ttccggcggg cgcgccggcg catgcagcct
      601 cggcattccc cgaccaccac gacgagcgat ccatgtcggt gcccgtgacc ccgcccgccc
      661 cggcggcccc gcgaaccgac gacgccgcgc gccggccgcc accacctccg gagaccgtcc
      721 tggaggtcgt gcgtgagacc ctgccgcggg tcggggccga tggccggttt ggcgcagaga
      781 acgtgttcgt gtcggcgatc tggcaccgca tcgcggacga ccgccggctg gtcgacctgt
      841 cgttcgatcg cttcaagcgc tggctggtga acgccaaccg tgacggctgg ctcgtgctcg
      901 cccgcgccga tctggtcggc gcgatggatc cccgacaggt cgccgagtcg gagatcgagg
      961 atcgcggcgc gaccttccac ttcgtcctcg atcagcaagc cgaggcgctc gcgtttcacc
     1021 aggggagcca tgctcgataa cacgcgactg cgcgccgagc tcacccatat ccaccatgcg
     1081 ctcagcgcgg tcgccgccag cccgcgcggc aagggcatgg tcgacgatct gcgcggcgcg
     1141 atcggcggga cgacggtcgc gatcctgtgg caggcgctgt tcgcggactg cctgcggctc
     1201 gcatacagcg cggtcgccgc ggacggcgag atcggcgacg acgagatcga cgcgctctac
     1261 gagctgctgt tctccgcggc gcgccactac gcgagggtga tccccgcgcg ctacagcgac
     1321 ttcaccgcgg tcgatcacga gctggcccgc gcgttcctgg agcgctacgc cgccgatagc
     1381 ggcccgttcg gacgcggcgc ggcgcagcac tggccgggcc tgacgctgtg ccgccgcgcc
     1441 gccgagctcg gtgacgacca cgcgctggag cgctacgagc ggacgatgac gtggctgatc
     1501 ggcgccgcat gtcaggtcgg cggcgtcacc gaggccgatc cgcgctggcg cggccgggtc
     1561 gacgagctcg acgatctgcg ccggaccctg gcgcgcgatg ccaaggtcga ggcccccgcc
     1621 gccgatctgc gggtcgaggc gttcctcgcg ccgacccgcg tgttcaccgc cgtcgagcag
     1681 gcgtcatcga tcttcgagaa cgatccgttc gatgtcgaga cgatccaccg cgaggcgcgc
     1741 gcggtgttcg agcagatggt ggagcgcgcc accacgccgt cgcagatcac cgatcgcggc
     1801 cggatgctgc tcgtgctcgg cgcctcggga gccggcaaga cccacctgct ccgcggcttc
     1861 cggcgccacg tccacgagta cggccgcggc ttcgtggtct acgcccagct gcagtcggaa
     1921 accgacgact acggccgcta cctgctccag cacctggtcg actcgctggc gcggccgtac
     1981 gccgggccgt ccggcgagcg gaccggcctg cacgagctcg cgagcgggct gccgcgactg
     2041 gtcggccagc cgctgaagtc tcgtatcgag tgcctggccg acgatacctg ggactcgccg
     2101 gccagcctca acgactacgt gaaccagctc accgacgacc tgctccgcga ggccgatctg
     2161 gcgagcttcg atcccgacct gctgcgcatc atgctctacg cgctccaccc cgatcagcgc
     2221 acgacatcgc gggtgtacaa gtacctgcgc tgcgaggaca tgggcgacta tgatcgcagg
     2281 tggatcggcg acgtcgtgcc gcgcaccggc gacgaccatc cctactggat gatccgcgag
     2341 ctcggccggc tggcagcggt gacccagggc gccgcgctcg tgctgatggt cgaccaggcc
     2401 gatctcgccg ggttcgaggc cgcgtcgggc cggatgttcc ggcgcgccat cgacacgctg
     2461 tatcgcatcg tcagcgagat cccgtccgcg atcgccgtgg tcgcgtgtct gtcgaacctc
     2521 tacgagaagg tgcgcgccga gctcagccgg ccagcgctcg accgcctgga gaaggatccg
     2581 ccgatcgcac agctccagat caaccgctcg tatgccgaga tcgaagcgat cgtcgcccgg
     2641 cggctgtcgt ggctgttcgc cgagcgcggc acggtgcatc ggccggacga gccggtctat
     2701 ccgatcccgg aggcccagct caagcagctg gagaaccggc gcacccgcga catcctggag
     2761 tggtgtcatc agctccaggc ccagtgtgcg gcggccggca ggatcgtcga gctcgacgac
     2821 ggcggcgcga tctcggatcc gccgccaccg ccgcggagcg acgccgatct cgaccacatc
     2881 gcggcggcgt ggaacgacgc gatgcacgcg cgtggcatcg acgtccccga cgaggaagac
     2941 gagatcctcg cggtgatcgg cgccgccgcg cgggtgtgcg ccgacgaggt cgggctgacg
     3001 ttgacagcgc cgccgcgcag gaacggcgcg ctccgcgtgc agctgtccgc ggcggccgag
     3061 tccgccgacc tggcgatcgc ggtcaccaat cgcggctacc agcgcggctc gttcgccggg
     3121 cagatcgagg cgctgcgcaa gaccgccgac aaggccaccc cggtcgcggt gcgcacgctc
     3181 aagtttcccc acggcgagac gtccgatcag gcgatcgccc agctgctcaa ggcgggtggc
     3241 cggcgggtct acgtcgatgc ctcggcgcta cgcacgctgg tcgcgttcca gcgcttccag
     3301 cccgcgttcc cacccgatcg cgtggcggcg tggcggcagc gcgaccggcc gatctcgacg
     3361 ctccctgcga tgatcgacat cttcgatctg gagcgcctgc gcagctcccc cgcagcggct
     3421 cccccacgag acccaccgcc gtcgagcacg ccggc
//
LOCUS       DASVOR010001516         3030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_161356,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001516 DASVOR010000000
VERSION     DASVOR010001516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3030
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161356"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VF516_28615"
     CDS             <1..328
                     /locus_tag="VF516_28615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111741.1"
                     /translation="LIAALDGALRAIDDLAAAVAIPEPLPVPVEVDPEADDEPAPRER
                     WEPAEPATPAAGPEPIVLSFPAEPRRYPSLQDTLRASRKRKRKRDDRAEEASVLRWGG
                     VVVATR"
     gene            complement(351..>3030)
                     /locus_tag="VF516_28620"
     CDS             complement(351..>3030)
                     /locus_tag="VF516_28620"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8111742.1"
                     /translation="APPAATPPAPAAPPRLVTSVEGISEYVLGNGLRVLLFADPSKDT
                     FTVNLTYLVGSRMEGYGETGMAHLLEHMLFKGSPRHPKIWEELEQRGAANNASTWYDR
                     TNYFETLPAKGDNLSWALEIEADRMIHASIAQADLDKEFSVVRNEFEIGENDPSEILS
                     ERMWSTAYLWHNYGKSTIGSRADIEKVPAISLKRFYKKYYRPDNAVLTVAGKFEAAAA
                     LDLIGKQFGPVTNPPAPIEPTYTTEPVQDGERIVTLRRNGDVQVVGLVYHVSAAPDPD
                     FAAVDALADVLTAEPSGRLYVALVKSGLATSVRSQLLPLHDPGVIELSATLPANKSID
                     AVRDKMIAVVEGFAKSKITDEEVARFKARSKKQFKLRFANSTQLGIALSEYIAAGDWR
                     LLFLHRDRVAQLTPADLQKAAAAYLVASNRTLGMFYPTKNPERAPQREIPDAAKVVAG
                     YKGAPPEAEGEKFEATLDNIEKRTTRSVLADGMKLAILAKQTRGHVVRARLTLRFGTE
                     ADFTGNRVPAAMLEDLIQRGTRHHTFQQLKDQWDLLEAQVSFASRPGLLDVNVQTTRD
                     HLAAVLALVDEVLREPTFPQDQFDIVVKDALTQLDDQKSDPQAQAFVAATRALSPYPV
                     NHPFYLPTTEESIAEIKALKLADVKKLPAMFGTSNATLTILGDVDTAAIKPWIERTWG
                     TWKSPRPWKRIARKYTATTAGEQVLDFPDKANALIAAVHAVDMKDDDPDAPAMAAADY
                     TLGGGGFVSRLLDRLRQKDGLSYFAFSSFQLDSLDPVGAFLAGGALNPEHAKQGMAAM
                     LEEIAKLTSGGVTAAELTTAKSGLQQNFDRNLSNDAFVLNLLHDGLYLDRKLDFWAKH
                     NAAIAALTVDQVNTAIKRHLKPEALVKITAGDRKKM"
BASE COUNT          405 a          990 c         1119 g          516 t
ORIGIN      
        1 gctgatcgcc gcgctcgacg gcgcgctgcg cgcgatcgac gatctggcgg cggcggtggc
       61 gatccccgag ccgctgccgg tgccggtcga ggtcgatccc gaggcggacg acgagccagc
      121 gccgcgggag cgctgggagc cggccgagcc cgccacgccc gccgcggggc ccgagccgat
      181 cgtcctctcg ttccccgcgg agcctcgccg gtatccgtcg cttcaggaca cgctgcgcgc
      241 gtcgcgcaag cgcaagcgca agcgcgatga tcgggccgag gaggcgagcg tgctccgctg
      301 gggcggcgtc gtggtcgcca cgcggtgagg tgtcgccgtc acatcttccg tcacatcttt
      361 tttcggtcgc ccgcggtgat cttgaccagc gcctccggct tcaggtggcg cttgatcgcg
      421 gtgttgacct gatcgacggt cagcgccgcg atcgccgcgt tgtgcttggc ccagaagtcg
      481 agcttgcggt cgaggtacag cccgtcgtgc aagaggttga gcacgaacgc gtcgttcgac
      541 aggttgcggt cgaagttctg ctgcaggccg gacttggcgg tggtgagctc ggcggcggtg
      601 accccgccgg aggtcagctt ggcgatctcc tccagcatcg ccgccatgcc ctgcttggcg
      661 tgctcggggt tgagcgcacc accggcgagg aacgcgccca cggggtcgag cgagtcgagc
      721 tggaacgacg agaacgcgaa gtacgacagg ccgtccttct ggcgcagccg gtcgagcagg
      781 cgcgacacga agccgccgcc gcccagcgtg tagtcggccg cggccatcgc gggcgcgtcg
      841 gggtcatcgt ccttcatgtc gacggcgtgg accgcggcga tcagcgcgtt ggccttgtcg
      901 gggaagtcga gcacctgctc gccggcggtg gtcgcggtgt acttgcgcgc gatccgcttc
      961 cacggccgcg gcgacttcca ggtgccccag gtgcgctcga tccacggctt gatcgccgcg
     1021 gtgtcgacgt cgccgaggat cgtcagcgtg gcgttgctgg tcccgaacat cgcgggcagc
     1081 ttcttgacgt cggcgagctt cagcgccttg atctcggcga tcgactcctc ggtggtcggt
     1141 aggtagaacg gatggttgac cgggtagggc gacagcgcgc gcgtcgcggc gacgaacgcc
     1201 tgggcctggg gatccgactt ctggtcgtcg agctgggtca gcgcgtcctt gaccacgata
     1261 tcgaactgat cctgcgggaa ggtcggctcg cgcagcacct cgtcgaccag cgcgagcacc
     1321 gcggcgaggt ggtcgcgcgt ggtctggaca ttgacgtcga gcaagccggg gcgcgacgcg
     1381 aagctgacct gggcctccag caggtcccac tggtccttga gctgctggaa cgtgtggtgc
     1441 ctggtgccgc gctggatcag gtcctcgagc atcgccgcgg ggacccggtt gcccgtgaag
     1501 tcggcctcgg tgccgaaccg cagggtgagc cgcgcgcgca cgacgtggcc gcgggtctgc
     1561 ttggcgagga tcgcgagctt catgccgtcg gccagcacgc tgcgcgtggt gcgcttctcg
     1621 atgttgtcga gcgtggcctc gaacttctcg ccctcggcct cgggcggcgc gcccttgtag
     1681 ccggcgacga ccttggcggc gtcggggatc tcgcgctggg gcgcgcgctc ggggttcttg
     1741 gtcggataga acatgccgag cgtgcggttg gacgccacga ggtaggccgc cgcggccttc
     1801 tggaggtcgg cgggggtgag ctgcgcgacg cgatcgcggt ggaggaacag caggcgccag
     1861 tcgccggccg cgatgtactc cgacagcgcg ataccgagct gggtgctgtt ggcgaaccgc
     1921 agcttgaact gcttcttcga cctggccttg aaccgcgcga cctcctcgtc ggtgatcttc
     1981 gacttcgcga agccctcgac caccgcgatc atcttgtcgc gcacggcgtc gatcgacttg
     2041 ttcgccggca gggtcgccga cagctcgatc acgccggggt cgtggagcgg cagcagctgc
     2101 gaccgcaccg aggtcgccag gccggacttg acgagcgcga cgtacagccg gcccgacggc
     2161 tcggcggtca gcacgtcggc gagcgcgtcg accgccgcga agtcgggatc gggggcggcg
     2221 gacacgtggt agacgaggcc gacgacctgg acgtcgccgt tgcggcgcag ggtcacgatg
     2281 cgctcgccgt cctgcaccgg ctcggtggtg taggtcggct cgatcggcgc cggcgggttg
     2341 gtcaccggcc cgaactgctt gccgatcaga tcgagcgccg cggcggcctc gaacttgccg
     2401 gcgacggtca gcaccgcgtt gtcgggccgg tagtacttct tgtagaaccg cttgagcgag
     2461 atcgccggca ccttctcgat gtcggcgcgc gagccgatcg tcgacttgcc gtagttgtgc
     2521 cagaggtacg cggtcgacca catccgctcg gacaggatct ccgacggatc gttctcgccg
     2581 atctcgaact cgttgcgcac caccgagaac tccttgtcga ggtcggcctg cgcgatgctg
     2641 gcgtggatca tccgatcggc ctcgatctcc agcgcccagc tcaggttgtc gcccttggcc
     2701 ggcagcgtct cgaagtagtt ggtgcggtcg taccaggtcg acgcgttgtt ggccgcgccg
     2761 cgctgctcca gctcctccca gatcttgggg tgcctgggcg agcccttgaa cagcatgtgc
     2821 tcgagcaggt gggccatgcc ggtctcgccg tagccctcca tgcgcgagcc gacgaggtag
     2881 gtcaggttga ccgtgaaggt gtccttcgat ggatcggcga acagcagcac gcgcagaccg
     2941 ttgccgagca cgtactcgga gatgccctcg acgctggtga ccagccgcgg cggcgcggcg
     3001 ggggccggcg gggtggcggc cggtggggcg
//
LOCUS       DASVOR010001517         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_483208,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001517 DASVOR010000000
VERSION     DASVOR010001517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483208"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1646
                     /locus_tag="VF516_28625"
     CDS             324..1646
                     /locus_tag="VF516_28625"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8111743.1"
                     /translation="MALQQVGKTSGVAADATGNGTAKQAGTPGKYTLVETLAKPAQSV
                     GALRAALVSTPALGAELASYFADGNEDANLNGLLGRAYAASAASAASATHTLGATKSA
                     APVAEKAGTGAGATLPADRSDTKTLAKGEYKWTYKAASSSSAQLIPEFTPDKTKVGAK
                     NVSFAQTVVNQVGAKKIYAGGTTAHPDLKKATFEPYEESTKKTRIDHAPSSENDPYYG
                     AEWDVGAKKWVKEPGAYSKVGSSVKGTSSSSARMDDTPDDGPVAREGQGNVEIAFETV
                     AVVLETREPLGSLKWGYKIEDKANAPLVLTGATKADCVDAPSAEWGAAMDKFYTAKFD
                     AIIDEFAAGKADLTPAHKLALDTLAAKLKADATLRAELAGAADLKEADPDKISDDRAK
                     AARDHLVSKGATAGQCTVVPLGSDWARVQTTAGKDEPKNRRVQIWVRK"
     gene            1649..2317
                     /locus_tag="VF516_28630"
     CDS             1649..2317
                     /locus_tag="VF516_28630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111744.1"
                     /translation="MRAIVAVWIALLRCSPGAPEPEQPREKGRVMSDTLSPALDAELA
                     EIRKDPGESVGFGFKADIPYVPNRADSLIASREPGVTERLIAEVHGPGDRVYRLAVLH
                     VLGKRADAAVDAALVGLLDDPELCATAAYLLGAAGYREYPARARDTEAVLAALRRHVD
                     DASRFVDPFHRKSFRTQDFVIAAYVRIAGPDRFTLANPDLVGHGLPAFTDAERAALLA
                     QIGK"
     gene            complement(2639..>3432)
                     /locus_tag="VF516_28635"
     CDS             complement(2639..>3432)
                     /locus_tag="VF516_28635"
                     /inference="COORDINATES: protein motif:HMM:NF013636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proprotein convertase P-domain-containing
                     protein"
                     /protein_id="HEX8111745.1"
                     /translation="MNIDPTDDCTFWFTSEYMGASGAFNWHTRIGTFKLPSCPINQPP
                     TVSITSPTSGSTVTGTITVSANASDPDGPIASVKFDLPDGTSVTDTAAPFSTTFDTTN
                     VANGSYTIQATATDNQSATAVATVTVTVHNCLDSTFSAKDTPSPIHDNDPLGLTSSIA
                     VTGNGNVGSLSLSLDIAHPFRSDLMVTLISPAGTQFSVSSRQGDSQDNLVIVDQAITA
                     FDGEALAGLWQLTVRDLAKGDAGTLNSWSLKLRDSCSPTAQRAGR"
BASE COUNT          558 a         1097 c         1281 g          496 t
ORIGIN      
        1 cgcgacgtcg cgatcgagat cgtcgtcgaa cgcgcggggc gccgtcgaca cctccgggcc
       61 gcacgccgcg gcgacgagga ccgcgagggc gagcaggccg gcgcgcatgg tccgagcgtg
      121 ccgcgccggc gatcgcggcg caagtttgcg gcggcgcgcg gggttcttcg gctcctctga
      181 aggacccgga tggcggtgtc ggtgtcttac acgtgcgccc gtggcccacg gtccgcgcgg
      241 gtgggccccg atccacatct ggcaccgatg gggagggcgg cgtcaccccg gccgtactac
      301 acccaaggca gcggaggtca ggtatggcac tgcaacaggt gggcaagacg agcggtgtcg
      361 cggcggacgc gaccggaaac ggcacggcga agcaggcggg cacgccgggc aagtacacgc
      421 tcgtcgagac gttggcgaag ccggcgcagt cggtcggcgc gctgcgcgcg gcgctggtgt
      481 cgacacccgc gctcggcgcg gagctcgcga gctacttcgc ggacggtaac gaggacgcga
      541 acctgaacgg cctgctcggg cgcgcgtacg ccgcgagcgc cgcgagcgcc gcgagcgcga
      601 cccatacgct cggtgcgacg aagtccgcgg cgccggtcgc ggagaaggcg ggcacgggtg
      661 cgggcgcgac gctgccggcg gatcgcagcg acacgaagac gctcgcgaag ggggagtaca
      721 agtggacgta caaggccgcc agcagctcgt cagcgcagct catccccgag ttcacacccg
      781 acaagacgaa ggtcggcgcg aagaatgtca gcttcgcgca gacggtggtc aatcaggtcg
      841 gcgcgaagaa gatctacgcc ggtggcacca cggcgcatcc cgatctcaag aaggcgacgt
      901 tcgagccgta cgaggagtcg acgaagaaga cgcgcatcga tcacgcgccg tcgtcggaga
      961 acgatccata ctatggtgcg gagtgggacg tgggcgcgaa gaagtgggtc aaagagccag
     1021 gcgcgtactc gaaggtcggc agctcggtga agggcacgtc gtcgtcgagc gcgcgcatgg
     1081 acgacacgcc ggatgacggc ccggtcgcgc gcgagggcca aggcaacgtc gagatcgcgt
     1141 tcgagacggt ggcggtcgtg ctcgagacgc gtgagccgct cgggtcgctg aagtggggct
     1201 acaagatcga ggacaaggcc aacgccccgc tcgtgctcac gggcgcgacg aaggcggact
     1261 gcgtcgacgc gccgtcggcg gagtggggcg cggcgatgga caagttctac accgcgaagt
     1321 tcgatgcgat catcgacgag ttcgccgcgg gcaaggcgga cctgacgccg gcgcacaagc
     1381 tcgcgctcga cacgctcgcg gcgaagctca aggccgacgc gacgctgagg gccgagctcg
     1441 cgggcgcggc ggacctgaag gaagcggatc cggacaagat ctccgacgat cgcgcgaagg
     1501 cggcgcgcga ccatctcgtg tcgaagggcg cgacggcggg tcagtgcacg gtcgtgccgc
     1561 tcggttccga ctgggcgcgg gtgcagacga cggcgggcaa ggacgagccg aagaaccgcc
     1621 gcgtgcagat ctgggtgcgc aagtagtcat gcgcgcgatc gtcgctgtgt ggatcgcgct
     1681 gctccggtgc tcgccgggcg cgcccgagcc cgagcagccg cgcgagaagg gtcgagtcat
     1741 gtccgatacg ctgtctccag cactcgacgc cgagctcgcc gagatccgca aggatccggg
     1801 agagtcggtc ggcttcgggt tcaaggccga tattccctac gtgcccaatc gggcggactc
     1861 gctgatcgcg agtcgcgagc cgggtgtgac cgagcggctc atcgccgaag tgcatgggcc
     1921 cggcgatcgc gtgtatcgcc tcgcggtgct gcacgtgctc ggcaagcgcg cggacgcggc
     1981 ggtcgacgcg gcgctcgtcg gccttctcga tgatccagag ctgtgcgcga ccgccgcgta
     2041 cttgctcggc gctgcggggt atcgggagta tcccgcgcgc gcgcgcgata ccgaagcggt
     2101 gctcgccgcg ctgcggcggc acgtcgacga cgcgagcagg ttcgttgatc cgttccatcg
     2161 caagtcgttt cgcacgcagg acttcgtgat cgccgcgtac gtgcgcatcg ccggaccgga
     2221 ccggttcacg ctcgcgaacc ccgacctcgt cggccacggg ttgcccgcgt tcaccgacgc
     2281 ggagcgcgcg gcgctgctcg cgcagatcgg gaagtaagct ctccgcacgc gcgcatgcgg
     2341 tggtcgctac gggcgactca gatcgaggtc ctcctgttcg caccagcatt tccaggtacg
     2401 gcggggcagc agccatgatc ttctccgaca agctcgagga agttaccctc ggtgcttccg
     2461 agcggcaacg cccgcaagcc gccgatccac cccaccgacc ggccctgcac agcgacgctc
     2521 tcgagcgacg cggcggctgc gaagcgccac cccggcccgc acgctggcgg cggcgcccgc
     2581 ggagttgtcc accgcgactt caagccctac gtgtggggcc aagcgccgga acgccagatc
     2641 aacggccagc tcgttgcgcc gtcgggctgc agctgtcccg gagcttcagg gaccacgagt
     2701 tcagcgtgcc agcgtctccc ttggcaagat ctcgaacggt gagctgccag agcccggcca
     2761 gcgcctcacc gtcgaatgcc gtgatcgcct ggtccacgat cacgaggttg tcctgggagt
     2821 cgccctggcg actgctgacg ctgaactggg taccggccgg ggagatcagg gtgaccatca
     2881 ggtcgctgcg gaaggggtgc gcgatgtcga gcgacagcga cagcgagccc acgttgccgt
     2941 tgccggtgac cgcgatcgac gaggtgaggc cgagagggtc gttgtcgtgg atcgggctcg
     3001 gtgtgtcctt cgcactgaac gtactgtcga ggcagttgtg gaccgtcacg gtcacggtcg
     3061 cgacggcggt ggcgctctgg ttgtcggtcg cggtcgcctg gatcgtgtag ctgccattgg
     3121 cgacgttggt ggtgtcgaac gtggtcgaga acggcgcggc ggtgtcggtg acgctggtgc
     3181 cgtcgggcag gtcgaacttc acgctggcga tggggccgtc ggggtccgag gcgttggccg
     3241 acaccgtgat cgtgccagtg accgtcgagc cgctggtcgg cgacgtgatc gagaccgtcg
     3301 gcggctggtt gatcgggcag ctggggagct tgaacgtgcc gatccgcgtg tgccagttga
     3361 acgcgccgct cgcgcccatg tactccgacg tgaaccagaa cgtgcagtcg tcggtcgggt
     3421 cgatgttcat cg
//
LOCUS       DASVOR010001518         1524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_394761,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001518 DASVOR010000000
VERSION     DASVOR010001518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394761"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /gene="metG"
                     /locus_tag="VF516_28640"
     CDS             <1..357
                     /gene="metG"
                     /locus_tag="VF516_28640"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HEX8111746.1"
                     /translation="AAAEPRSAALPPIKIDDFARIELRVGTVLSAAAVPKAKKLLQLS
                     VDLGEPAPRSIVAGIAETYAPDQIVGKQVIVVANLEPATIRGIASQGMLLAAGEAQVL
                     GLSALDRHVPPGTRVR"
     gene            369..803
                     /locus_tag="VF516_28645"
     CDS             369..803
                     /locus_tag="VF516_28645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8111747.1"
                     /translation="MSELRRAARLVVNAPAIVESIGQGPMHLHPNLAAVFRRVEADPT
                     TVGQRFPAVVRDLSTNGAFISAEPLPLLSRVAVKFELKAIGSVDAVGWVLWQRAEDCE
                     LPAQTGQTGQTGMITLPKGFGVLFESIPLEIRSAIAALVSRS"
     gene            800..1276
                     /locus_tag="VF516_28650"
     CDS             800..1276
                     /locus_tag="VF516_28650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111748.1"
                     /translation="MTARSFAFRPRYRALAWSSIGVGGAVAAVAAALGPVTVPLITGA
                     LGIALGAAYLRSPTWRIAVTIDDSGLRVGSPNKQRFQLAWDEVIRVVASPSTRSCFVD
                     GGSPERSLLVPGDGAPAPYDIADRAALFEAILAHVPADKVQTVATLDQADPAPARR"
BASE COUNT          209 a          557 c          521 g          237 t
ORIGIN      
        1 gccgcggccg agccgcgctc cgcggcgctg ccgccgatca agatcgacga cttcgcccgg
       61 atcgagctgc gggtcggcac cgtgctgtcc gccgccgccg tccccaaggc caagaagctg
      121 ctccagctct cggtcgacct cggagagccg gcgccgcgct cgatcgtcgc cggcatcgcc
      181 gagacctacg cgcccgatca gatcgtcggc aagcaggtga tcgtcgtcgc caacctggag
      241 ccggcgacga tccgggggat cgcgtcgcag ggtatgctac tcgccgccgg cgaagctcag
      301 gtgctcggac tttccgcgct cgatcgccac gtgcccccgg gaacgcgggt acgataggcg
      361 ggctggatat gtccgagctg cgccgcgctg cacgcctggt cgtcaacgcg cctgcgatcg
      421 tcgagtcgat cggccagggc ccgatgcacc tgcaccccaa cctggcggcg gtgttccggc
      481 gggtcgaggc cgatccgacc acggtcggcc agcgcttccc cgccgtggtc cgcgacctgt
      541 cgaccaacgg cgcgttcatc tcggccgagc cgctgccgct cttgtcgcgc gtcgcggtca
      601 agttcgagct caaggccatc ggctcggtcg acgccgtcgg ctgggtgctg tggcagcgcg
      661 ccgaggactg cgagctgccg gcccagaccg gccagaccgg ccagaccggc atgatcacgc
      721 tgcccaaggg cttcggcgtg ctgttcgaat cgatcccgct ggagatccgg tcggcgatcg
      781 ccgcgctggt ctcgcggtcg tgaccgcgcg gtcgttcgcg ttccgcccgc gctaccgcgc
      841 cctggcctgg agctcgatcg gggtcggcgg cgcggtcgcg gcggtcgccg ccgcgctcgg
      901 gccggtgacc gtcccgctga tcaccggcgc gctcggcatc gcgctcggcg cggcgtacct
      961 gcgctctccg acctggcgga tcgccgtgac catcgatgac agcgggctgc gggtcggctc
     1021 gccgaacaag cagcgctttc agctcgcctg ggacgaggtc atccgcgtcg tcgcctcgcc
     1081 atcgacccgc tcgtgcttcg tcgacggcgg cagcccggag cgcagcctgc tggtgcccgg
     1141 cgacggcgcg cccgcgccgt acgacatcgc cgaccgggcg gcgctgttcg aggcgatcct
     1201 cgcccacgtc ccggccgaca aggtccagac cgtcgcgacg ctcgaccagg ccgatcccgc
     1261 accggcccgc cgctgaatga agagctgtac atcgaggctg tggaccttcg tcccgcagac
     1321 gttgagcttg acttcgacgt cgacgcaagg cgttgagctt gacggcaacg tcgacgtctg
     1381 acgccaccgt cgacgttgaa cgtggaccca gcactgccgg tgagaatcgt cgtcgaggag
     1441 acaccccgga aatcaacgtc gaggtcgacg gtggtgtcta tgtccaacgt cgccgtcgac
     1501 atcaacgttc gggtcaaggt catc
//
LOCUS       DASVOR010001519         2503 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_220219,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001519 DASVOR010000000
VERSION     DASVOR010001519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2503
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220219"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VF516_28655"
     CDS             complement(<1..631)
                     /locus_tag="VF516_28655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111749.1"
                     /translation="MRRVLIVALTLAAGQAEARPGRSAPHDDPAASALWREVIEPHGT
                     EVAALVARARTAMARPAESADSTAGRRLRYAYGLLRRAHQLSPDNTDVLSLLGSAADD
                     LGKTRQAIEALEDCIRLQGPEHAGSEVTDRLGTIYLRLGKLDDAVRWLRYAIDPIAGS
                     SSPAVAVHLATALAARGQMSDAIDVLSNALPPAAGSYYTDPVTLVSFALA"
     gene            complement(807..1778)
                     /locus_tag="VF516_28660"
     CDS             complement(807..1778)
                     /locus_tag="VF516_28660"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HEX8111750.1"
                     /translation="MDALWVVVAIAMLDLLLIVHECGHYLVARGCRIRIERFSIGFGP
                     PIVERTSQATGTTLRIGLIPLGAFVRIRGMDDADADPVDPRAYPNRPVWQRLATILAG
                     PAASYLSVAVLAIALYTCHGVDVPRWYGINRVVNGSPAADKLEPGDTVLAFDHAPLTI
                     DSRPTLSERVNRGSGAPITLTIERNGEQREVVIEPKQGKDASGRRVWLIGVNLEARDL
                     TVQLGLLDAARRGLEYPVAQARLIATALYRVVLGDERADPGGPIRMIYELHQAFQFGI
                     SEGIKLEMMLGVYIGLVLALPLPSFDGGRLLLLIYRAMTRRRSRSAR"
     gene            2023..>2503
                     /locus_tag="VF516_28665"
     CDS             2023..>2503
                     /locus_tag="VF516_28665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111751.1"
                     /translation="MDRGVIATANELFEARHYPGVVAMCSDALDDEPECVPLLLIRAR
                     ARMALRRDLDAQADLRDIIRLDPRCSLAYRLLGELAARRDENASAAVFFCEALRLDPD
                     DREAHDWLQIVAAPRPAAAANQPPATAAAAGRLSPSTRAPSPSPARLARGTQPPDRPE
                     "
BASE COUNT          359 a          887 c          904 g          353 t
ORIGIN      
        1 ccgcgagcgc gaagctgacc agcgtcaccg ggtcggtgta gtagctgccg gccgcaggcg
       61 gcagcgcgtt gctcagcacg tcgatcgcgt ccgacatctg gccgcgcgcc gccagcgcgg
      121 tcgcgaggtg gaccgcgacc gccgggctgc tcgaccccgc gatcgggtcg atcgcgtagc
      181 gcagccagcg gaccgcgtcg tcgagcttgc ccagccgcag gtagatcgtg ccgagccggt
      241 cggtgacctc ggacccggcg tgctcgggcc cctgcagccg gatgcagtcc tccagcgcct
      301 cgatcgcctg gcgcgtcttg ccgagatcgt cggcggcgga gccgagcagg ctcaacacgt
      361 cggtgttgtc gggcgacagc tggtgggcgc ggcgcagcag gccataggcg tagcgcagcc
      421 ggcgcccggc ggtgctgtcc gccgactccg cgggcctggc catcgcggtc cgggcgcgcg
      481 ccacgagcgc cgcgacctcg gtcccgtgcg gctcgatcac ctcgcgccac agcgcgctcg
      541 cggcgggatc gtcgtgcggc gcggatcgac ccggcctggc ctcggcctgg ccagccgcca
      601 gcgtcagggc aacgatcagg acgcggcgca ccgatcgagt atgcagggcg aagccggcgg
      661 gtgccagggt gctgcggggg atgcaccctg gccgccgcta gaaaaatcaa tatcttgcac
      721 agatggccac gcgccgacga tcgcgaatgg ccgggcgctg tcgcgctgtg ccggcgcggc
      781 gccatgtacc ccgcggtgga cgccggtcag cgcgcggacc tggagcgccg ccgggtcatt
      841 gcccggtaga tcaggagcag gaggcggccg ccgtcgaacg acgggagcgg cagcgcgaga
      901 accaggccga tgtagacacc cagcatcatc tcgagcttga tgccctcgga gatgccgaac
      961 tggaacgcct ggtggagctc gtagatcatg cggatcggcc cgcccggatc cgcacgctcg
     1021 tcgccgagca ccacgcggta gagcgcggtg gcgatcagcc gggcctgcgc gaccgggtac
     1081 tcgaggcctc gccgagcagc gtcgagcagc ccgagctgga ctgtgaggtc ccgtgcctcg
     1141 agattcacgc cgatgagcca gaccctcctg ccgctggcat ccttgccctg cttcggctcg
     1201 atcacgacct cgcgctgctc gccgttgcgc tcgatcgtga gcgtgatcgg cgcgccgctt
     1261 ccccggttga ctcgctcgct cagggtcggt cggctgtcga tggtcagcgg cgcatggtcg
     1321 aacgcgagga cggtgtcgcc gggctcgagc ttgtccgcgg cgggagagcc gttcaccacc
     1381 ctgttgatgc cgtaccagcg cgggacgtcg accccgtggc aggtgtagag cgcgatcgcg
     1441 agcacggcga ccgacaggta gctcgcggcg ggcccggcga ggatcgtggc gaggcgctgc
     1501 cagaccgggc ggttggggta ggcgcgtggg tcgacgggat ccgcgtccgc gtcgtccatg
     1561 ccgcgaatcc ggacgaacgc gccgagcggg atcagcccga tgcgcagcgt cgtgccggtg
     1621 gcctgcgacg tgcgctcgac gatcggcggt ccgaatccga tgctgaaccg ctcgatccgg
     1681 atccggcacc cgcgcgccac caggtagtgg ccgcactcat ggacgatgag caaaaggtcc
     1741 agcatcgcga tcgcgaccac cacccagagc gcgtccatgg caaggactct acccccttcg
     1801 tcgaggtgga tcggcgcgag acgctgaacc gccgcagcct cccgtcgacc tggcgaagtt
     1861 gctcgggatc cggatcgcag catcttcgat cggcgtggat gccgggtgat gtacccccag
     1921 gtcggggtag tgcagtcacc gctcagagac cgggtagaat gcagtgattt cgaatatcta
     1981 accaggtggc gggctggccc gtggcttgct gccaccggag gtgtggaccg cggagtcatc
     2041 gcgacagcca acgagctgtt cgaggccagg cactacccgg gggtggtcgc gatgtgctcg
     2101 gacgcgctcg acgacgagcc cgagtgcgtg ccgctgctgc tgatccgcgc ccgggcgcgc
     2161 atggcgctgc ggcgcgatct cgacgcccag gccgacctgc gcgacatcat ccgcctcgac
     2221 ccacggtgca gcctcgccta ccgcctgctc ggcgagctcg cggcgcggcg cgacgagaac
     2281 gcatccgccg ccgtgttctt ctgcgaggcg ctgcgcctcg atcccgacga tcgcgaggcc
     2341 cacgactggc tgcagatcgt cgccgctccc cgacccgccg cggcagcgaa tcaacccccg
     2401 gcaaccgctg ccgcggctgg tcggctttct ccctcgacgc gcgcgccgtc gccgagcccg
     2461 gcgcggctcg cccgcggcac gcagccgccg gatcggccgg agc
//
LOCUS       DASVOR010001520         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_571396,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001520 DASVOR010000000
VERSION     DASVOR010001520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571396"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="VF516_28670"
     CDS             <1..1786
                     /locus_tag="VF516_28670"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8111752.1"
                     /translation="GGDILDYRRRITELGPRQFFDTVSTIRGVVGVTGSVPASLGMFE
                     NWKYEVSFNYGETHSLVGTAGQLLVPRLADALGPSMRDASGTPICVRVPGDPSTKITY
                     NVPLASGTTLHVACVPLNLFAPAGMIPSDQLQSLTFSDAGAGIDNLRTLLATASGQVA
                     QLPDHGGVSLSLGVDARDETGSQAPPSVASAGYTTDNEAHATEGQFEVYEGFGELAIV
                     PITGGDLAQRVELDLGARALHHTRFGSHLTYKAGGLVRTAFGIAARGTYATAFRAPSV
                     FDLVGGRTERLPTAEDPCDTRPPSAGDSTRTLDPMIQAQCTAQGVPAGTRFTTSQQPS
                     VIGGNPDLQAETAATTTVGVVVEPPQLKGLALTADYWHIAIDHAIETLGVQTIFANCY
                     ERGIQAFCDQVHRDFNTHRILHVDQFLQNVPRTVTSGIDVALWYDARLAELGRIHTGL
                     EAQYLLRYDLQTGSQVIHGVGFYDLGVYPRYKANLSSNWVHPGGASGGFLLRYVGSYQ
                     ECAGNDCNNPRNLAMASRDVDRYFKLDLFGGYDFPSSLGKTTLQLGVNNVFDATPPVV
                     YNAPAANSDPTTYDFVGRMVYLRMTQLF"
BASE COUNT          301 a          812 c          602 g          275 t
ORIGIN      
        1 cggcggcgac atcctcgact accggcgccg gatcaccgag ctcggcccgc ggcagttctt
       61 cgacaccgtc agcacgatcc gcggcgtcgt gggcgtcacc ggcagcgtcc ccgcgtcgct
      121 ggggatgttc gagaactgga agtacgaggt gtcgttcaac tacggcgaga cccattcgct
      181 cgtcgggacc gccggccagc tgctcgtgcc ccggctcgcc gatgcgctcg ggccgagcat
      241 gcgcgacgcc agcggcaccc cgatctgcgt ccgtgtgccg ggcgacccgt cgaccaagat
      301 cacctacaac gtgccgctcg ccagcggcac caccctccac gtggcgtgcg tcccgctcaa
      361 cctgttcgcg cccgccggca tgatcccgag cgatcagctc cagagcctga cgttcagcga
      421 cgcgggcgcc gggatcgaca acctgcgcac gctgctggcc accgcgagcg gccaggtcgc
      481 ccagctgccc gaccacggcg gcgtctcgct gtcgctcggc gtcgacgcgc gcgacgagac
      541 cggcagccag gccccgccga gcgtggcgag cgccggctac accaccgaca acgaggccca
      601 cgccaccgag ggccagttcg aggtctacga gggcttcggc gagctggcga tcgtcccgat
      661 caccggcggc gatctcgccc agcgcgtcga gctcgatctc ggcgcccgcg cgctccacca
      721 cacgcgcttc ggctcgcatt tgacctacaa ggccggcggc ctggtccgca ccgcctttgg
      781 catcgccgcg cgcggcacct acgccaccgc gttccgcgcc ccgtcggtgt tcgatctcgt
      841 cggcggccgg accgagcggc tgcccaccgc cgaggacccg tgcgacaccc ggccgcccag
      901 cgccggtgac agcacccgga ccctcgaccc gatgatccag gcccagtgca ccgcgcaggg
      961 cgtgccggcc ggcacccggt tcaccaccag ccagcagccg tcggtgatcg gcggcaaccc
     1021 cgacctccag gccgagaccg ccgccaccac cacggtcggc gtcgtggtcg agccgcccca
     1081 gctcaagggc ctcgcgctca ccgccgacta ctggcacatc gccatcgatc acgcgatcga
     1141 gaccctcggc gtccagacca tcttcgccaa ctgctacgag cgcggcatcc aggcgttctg
     1201 cgaccaggtc caccgcgact tcaacaccca ccgcatcctc cacgtcgacc agttcctgca
     1261 gaacgtcccc cgcaccgtca cgtccggcat cgacgtcgcg ctgtggtacg acgcccggct
     1321 cgccgagctc ggccggatcc acaccgggct cgaggcccag tacctgttgc gctacgacct
     1381 ccagaccggg agccaggtga tccacggcgt cgggttctac gacctcggcg tctatccccg
     1441 gtacaaggcc aacctgtcga gcaactgggt ccaccccggc ggcgcgtccg gcggcttctt
     1501 gctgcgctac gtcggctcgt accaggagtg cgccggcaac gactgcaaca acccccggaa
     1561 cctcgcgatg gcgtcgcgcg acgtcgatcg ctatttcaag ctcgacctgt tcggcggcta
     1621 cgacttcccg tcctcgctcg gcaagaccac cctgcagctc ggcgtcaaca acgtgttcga
     1681 tgccaccccg ccggtggtct acaacgcccc cgccgccaac tccgacccca ccacctacga
     1741 cttcgtcggc cgcatggtgt acctccgcat gacccagctg ttctgacggt tcttgacagg
     1801 cgccccggcg cacgtcgctc gaccacccgc acatgatgac ccccacgcag agcatgggca
     1861 tgacgaagca cgctgcgatc ctgtgcctgg cggccgtcgc cgtcccggcc gccgccgacg
     1921 accacgcgcc gagctcgctg cgcatgcgcg agccgccgcg cacccggagc ccgcttccgc
     1981 tcggccgccg
//
LOCUS       DASVOR010001521         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_103939,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001521 DASVOR010000000
VERSION     DASVOR010001521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103939"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1713
                     /locus_tag="VF516_28675"
     CDS             <1..>1713
                     /locus_tag="VF516_28675"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HEX8111753.1"
                     /translation="GREAAVGHAREMYDALSSKSGLRPDVFALFTPANRLAFVAPPRA
                     LDDQALPAIAAVEKARGGSAFAHRIQVIDGVPYQMSALPIRGPREQVVGGMLVGVKLE
                     RLFAEFADQSDDQIDAQIRPTLIDGTTILASAWPADHRAELARAMQPDRTVRVTLGDD
                     TRDVIRLKDGDYDYYSDDAFEGYKAGDAGSVGRLVITRSRATLVDPASHLPWLEIASA
                     IAASVIIALGMGLLITRPIKQFVRQSGDLLQGDTNLTHRLVIRSHDEVSDLADNINQV
                     FERLHQLASGVQSAAFQVGASSAEISAASRQMLAGLKDQTLKIESSTTAVTELSASIQ
                     QVAGNAAQATDVAEQSSTAVTAAVERMESIRAAVADAADKMRELGESSKRIGNIVEVI
                     RQISEQTSMLALNASIEAAHAGEQGRGFAVVADEVSSLARRVGQSAKDIETLIQTVKE
                     QTQAVMASMDVGTREVASGSQLVTGTLNGLGRLISVVKDTAGAVHEQAVVSDEIARNM
                     DSVRQIATEVLSGSEESVVQAERLHELAFELEESIGGFNLDGQKLAAQNPARPARAAG
                     GGAGP"
BASE COUNT          264 a          591 c          645 g          213 t
ORIGIN      
        1 ggggcgcgag gcggcggtcg gccacgcgcg cgagatgtac gacgcgctgt cgtcgaagtc
       61 cgggctgcgg cccgacgtgt tcgcgctgtt caccccggcc aaccggctgg cgttcgtcgc
      121 gccgccgcgg gcgctcgacg accaggcgct gccggcgatc gcggcggtcg agaaggcgcg
      181 cggcggcagc gcgttcgccc accggatcca ggtgatcgac ggcgtgccgt accagatgtc
      241 ggcgctgccg atccgcggcc cgcgcgagca ggtggtcggc ggcatgctgg tcggggtcaa
      301 gctcgagcgg ctgttcgccg agttcgccga ccagagcgac gaccagatcg acgcccagat
      361 ccggccgacg ctgatcgacg gcacgaccat cctggcctcg gcgtggccgg ccgatcaccg
      421 cgccgagctc gcgcgcgcga tgcagcccga ccggaccgtc cgggtcacgc tgggcgacga
      481 cacgcgcgac gtgatccggc tcaaggacgg cgactacgac tactacagcg acgacgcgtt
      541 cgagggctac aaggcgggcg acgccggctc ggtcggccgg ctggtgatca cgcgcagccg
      601 ggcgacgctg gtcgatccgg cgtcgcacct gccgtggctc gagatcgcca gcgcgatcgc
      661 cgcgtcggtg atcatcgccc tcggcatggg cctcctgatc acgcggccga tcaagcagtt
      721 cgtccggcag tcgggcgacc tgctccaggg cgacaccaac ctgacccatc gcctggtgat
      781 ccgctcgcac gacgaggtgt cggacctcgc cgacaacatc aaccaggtgt tcgagcggct
      841 gcaccagctc gccagcgggg tccagtcggc ggcgttccag gtcggcgcgt cgagcgccga
      901 gatcagcgcg gcgtcgcgcc agatgctggc cgggctcaag gaccagacgc tcaagatcga
      961 gagctcgacc accgcggtga ccgagctgtc ggcgtcgatc cagcaggtcg ccggcaacgc
     1021 cgcgcaggcg accgacgtcg ccgagcagtc gagcacggcg gtgaccgcgg cggtcgagcg
     1081 catggagtcg atccgcgccg cggtggccga cgccgccgac aagatgcgcg agctcggcga
     1141 gagcagcaag cggatcggca acatcgtcga ggtgatccgc cagatcagcg agcagacctc
     1201 gatgctggcg ctcaacgcgt cgatcgaggc ggcgcacgcc ggcgagcagg gccgcggctt
     1261 cgcggtggtc gccgacgagg tgtcgtcgct ggcgcgccgg gtcggccagt cggccaagga
     1321 catcgagacc ctgatccaga ccgtcaagga gcagacccag gccgtgatgg cgtcgatgga
     1381 cgtcggcacg cgcgaggtcg cgagcggctc gcagctggtc accggcacgc tgaacggcct
     1441 cggccgcctg atcagcgtgg tcaaggacac cgcgggcgcg gtccacgagc aggcggtcgt
     1501 gtccgacgag atcgcgcgca acatggactc ggtgcgccag atcgcgaccg aggtgctgtc
     1561 gggctccgag gagtcggtcg tgcaggccga gcggctgcac gagctggcgt tcgagctcga
     1621 ggagtcgatc ggcgggttca acctcgatgg ccagaagctc gcggcgcaga atccggcacg
     1681 gccggcgcgc gcggcgggcg gcggcgccgg gcc
//
LOCUS       DASVOR010001522         1713 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_220268,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001522 DASVOR010000000
VERSION     DASVOR010001522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220268"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..942
                     /locus_tag="VF516_28680"
     CDS             <1..942
                     /locus_tag="VF516_28680"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8111754.1"
                     /translation="GGRSTTAIRADGSRTPLPSVAEHPVAPPTTQLETVTLQDHAQFA
                     AVTRPHAFDPKVRYPVVLRFGGAPDTRSVVDALDTYALDQWYADHGFIVVRSDGRGTP
                     GPERAWQRAIAGDVLTIPMTDQIDALKRLGAHHPELDTGRVGALGGEVGGYLAAMAVL
                     LHPDTFAAAVAVSPITDWSLVDAAYAERTMKAPAFNAEGYRRTTAAVYADHLTRPLLL
                     LPALPGSRIATAHAFQLLDALSAAGKHAEIATWPDRADAAHRIAATRLVLDFFRQRLG
                     PPVRPQVMPAAKTDDDDEEEERERAKRGSEGADKDRH"
     gene            complement(986..1288)
                     /locus_tag="VF516_28685"
     CDS             complement(986..1288)
                     /locus_tag="VF516_28685"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8111755.1"
                     /translation="MAASDLNEPMLAVARHTVGATADVAWARANMCDLQFGDAGFDAV
                     VCQFGLMFVSDKLAAVREARRVLRPGGSFLVTTWAPLDRNPIIDLAHRLRHGVTAA"
BASE COUNT          276 a          656 c          561 g          220 t
ORIGIN      
        1 ggcggccggt ccacgaccgc gatccgcgcc gacggctcgc gcaccccgct gccgtcggtc
       61 gccgagcacc cggtcgcgcc accgaccacc cagctcgaga ccgtcacgct gcaggaccac
      121 gcgcagttcg ccgcggtgac ccggccgcac gccttcgatc ccaaggtccg ctacccggtc
      181 gtcctgcggt tcggcggcgc gcccgacacc cggagcgtgg tcgacgcgct cgacacctac
      241 gcgctcgacc agtggtacgc cgaccacggc ttcatcgtcg tacgcagcga cggccgcggc
      301 acgccgggcc ccgagcgcgc ctggcagcgc gcgatcgccg gcgacgtgct caccatcccg
      361 atgaccgatc agatcgacgc gctcaagcgg ctcggcgcgc accaccccga gctcgacacc
      421 ggccgggtcg gcgcgctcgg cggcgaggtc ggcggctacc tcgccgcgat ggccgtcctg
      481 ctccaccccg atacgttcgc cgccgcggtc gcggtgtcgc cgatcaccga ctggtcgctg
      541 gtcgacgccg cgtacgccga gcgcaccatg aaggcgccgg cgttcaacgc cgagggctac
      601 cgccggacca ccgccgcggt ctacgccgac catctcaccc ggccgctcct gctcctgccc
      661 gcgttgcccg gcagccggat cgccaccgcg cacgcgttcc agcttctcga cgcgctgtcg
      721 gccgccggca agcacgccga gatcgcgacg tggccggacc gcgccgacgc cgcccaccgg
      781 atcgcggcga cccggctcgt gctcgacttc ttccgccagc ggctcggccc gccggtgcgg
      841 ccgcaggtca tgccggcggc gaagaccgac gatgacgacg aggaagagga gcgcgagcgc
      901 gccaagcgtg gcagcgaggg cgccgacaag gatcggcact gaccatcatt caccgacgcc
      961 tcggtcgatc ccttccaacg cgaacctacg ccgcggtcac gccatggcgg aggcgatggg
     1021 cgaggtcgat gatcgggttg cgatcgagcg gcgcccaggt cgtgaccagg aagctgccgc
     1081 ccggcctcag cacccggcgg gcctcgcgca ccgccgccag cttgtcggag acgaacatca
     1141 acccgaactg gcagaccacc gcatcgaacc cggcatcgcc gaactgcagg tcgcacatgt
     1201 tcgcgcgcgc ccacgcgacg tcagccgtgg cgcccaccgt gtggcgcgcc accgccagca
     1261 tcggctcgtt cagatcggac gctgccactg gcgcttcata aacaggacat cgggacaaac
     1321 gcagtcgatc accgagcatg tcgggccagt gcgatcgcca tcgataaacg tcgctaccag
     1381 ccagccgacg acagcggtaa ggttctgcag aacgcgtgac cccgctgcca cccctgtgga
     1441 cagcgcgccg cagcgaacac cgtcgactag gcgccctgcc cacagggctg tcagcgctaa
     1501 cgcccgcacg gatgctcctg gtcaggattg atttgatttt ctctgttccg ggaaacaaga
     1561 ttatgattat aaatcctgcc gggggaatgc gcgcgagcgt cgggccggtg ggcgggggcg
     1621 aaaaggcctg tgagctgccg cgccgtggtt gaccgcgatg gctgcggcgc gcaggtcaca
     1681 ggccgagcca ccgcccaccg gcagcacatg gtc
//
LOCUS       DASVOR010001523        10089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_483249,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001523 DASVOR010000000
VERSION     DASVOR010001523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10089
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483249"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /gene="fdhE"
                     /locus_tag="VF516_28690"
     CDS             <1..318
                     /gene="fdhE"
                     /locus_tag="VF516_28690"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HEX8111756.1"
                     /translation="GPGSGDEAPQRLLGCGICGAERPLDRIACAGCGQTEPTKLAVFQ
                     TERHPAVRIEACDACQRYIKAIDLTLDGRAIPEVDDLCSLSLDLWATEQGYERLEPSL
                     AGV"
     gene            610..2214
                     /locus_tag="VF516_28695"
     CDS             610..2214
                     /locus_tag="VF516_28695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111757.1"
                     /translation="MLVALLASVDGCATDGSDASDTNDAADDSTRDGNMEDESTIASD
                     LDAVFALHGDDHHNRLPNNVPVGDPTGVFTTVSSHGFIDLNNEFFQDLGTNGRRCVSC
                     HVPTSGWTITPRQLQTVFDATDGGVFDDGFGLSAVFRTVDGANAPNADVSTLAKRRQA
                     YSLLLNKGLIRIHLAIPTDAEFELVAVDDPYHFATADNMSLYRRPPPTTNLKFDSTIM
                     WDGRENAPGQTIAQDLATQANDALLTHAQGKGLTQAQRTSIVNFETALASAQIFDRTA
                     GELHAAGAQGGPDAILAQPFHIGINDNFGDPVTGAPFNPVVFTIFDRWTNIGLGKDCD
                     HDHDHDRDDRCRCDSDDRGHGHGYSYGRDRDCDRDDGINAARRAVARGQAIFNTRPII
                     ISGVSGINDEAAFGKPASLTGTCTTCHDTPNSGNHSVAAPLNIGLADESRRTPDMPLY
                     TLRNKTTHEIVKVTDPGRALVDGKWAHIGRFKGPILRDLAPRAPYFHNGFAKDLDAVV
                     DFYEDRFHLNLSKQERSDLIAFLRSL"
     gene            complement(3457..4383)
                     /locus_tag="VF516_28700"
     CDS             complement(3457..4383)
                     /locus_tag="VF516_28700"
                     /inference="COORDINATES: protein motif:HMM:NF015150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEX8111758.1"
                     /translation="MVASQYILPLVAISRGDFCRGAPCERAELASRVVREFEDNRCLL
                     VGDAIPRPVTRRFHALLRRLFALPAEEKLAYMATDPLDLGFGAYGSEHALDTGIANLL
                     ESWDVSLTAPTRWPATMLDDWAFLVELHEHLNRLVVDVLAALAVGLQVAPKELLTLLE
                     RRPGRIHLIHYPPMPDSVDTLARRQSKHCDMSIVTLLTPPTGPGLQACNADGRFLPVE
                     IGEDQCLIQAGLYLERLTGDRLRASRHTVETPSTEENSERYASPLFFSPPPDEHVSVL
                     RPFQSTTMLEKYPTLPAREISADYFKKLSGER"
     gene            complement(4431..6143)
                     /locus_tag="VF516_28705"
     CDS             complement(4431..6143)
                     /locus_tag="VF516_28705"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8111759.1"
                     /translation="MTSTRTMFLLGINSVYHESSAALIVDGRVVAAAEEERFNRVKHG
                     KPARVDNAHELPEAAIRFCLESAGIEARQLDAVCYSFDRTLRRKTFQLDPQSKRGDWG
                     DAAGEESFLASLHRVPGAVSQVLSHEVDAAFHWVPHHLAHAASTYYPSGFDDAAVLIV
                     DGIGEQATTFLAAGTGRELTYLKHLSYPHSLGFLWEKVCKYLGFGEYDACKVMGLAGY
                     GRREDAAPQFGSFVVVENDGFSIDAEVMEFRLDDFSPLERILGPRRRPEDPLIARHID
                     TAAALQAINDQAMLALAQAVYKLHPSDALCMAGGVALNCTSNWVVKEKGPFEKVYIPA
                     APHDAGTAVGAALHVYHHVLAGPRTTDVMAHPYQGPRYLDEEIRALLAAKDIRAIQVD
                     GIAARAADAIARGLVVGWFQGAMEFGPRALGNRSLLADPRDKNIREILNVKVKHREHF
                     RPFAPSVLEEHADAWFELGRPSQSYQAMLFACPVRSHVLDRIPAVLHIDRTARVQVVT
                     REHNPLFHQLIEHFHVRTGVPLVLNTSFNDSEPIVCSPNDALATFRSTRIDALAIGNY
                     WITR"
     gene            complement(6168..6974)
                     /locus_tag="VF516_28710"
     CDS             complement(6168..6974)
                     /locus_tag="VF516_28710"
                     /inference="COORDINATES: protein motif:HMM:NF039614.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase-related protein"
                     /protein_id="HEX8111760.1"
                     /translation="MIRLMCAPTYFDIEYSINPWMNVAVKVNKALAFEQWHHFIDVLK
                     ELGDTVEMIEPMPGLPDMTFAGDGGLVVDRTFIPSNFRNQERRGESAHYAHWFKQRGF
                     NIVRMPDDVFFEGLGDVVFHDRRAIVGHGRRSDARSIEHLKRIVPDLHILGDLHIRDD
                     RYFHLAMALGFIDADTLLYYPEAFDEDGVRRLSSMVKYPIALSDKDANEYFACNNLVI
                     GDTVLMDNCTAGLRGALAERGYQVTTCDMSEFKKSGGSLRCLVLSFIEQP"
     gene            complement(7653..7823)
                     /locus_tag="VF516_28715"
     CDS             complement(7653..7823)
                     /locus_tag="VF516_28715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111761.1"
                     /translation="MLGLGIWMSIAAASSLPAAAWLLRRVSVIVGYSPRDDTTAAATS
                     GATYARSEKWLS"
     gene            8027..8302
                     /locus_tag="VF516_28720"
     CDS             8027..8302
                     /locus_tag="VF516_28720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111762.1"
                     /translation="MSFVEHLPRNDTETLIALSGQTCVAYRVRAWPLGSPNPVDGGSV
                     SRDARPEATPALPALCQNRRDPTAPSGPQRDSTAKKHLKGLQCVDNT"
     gene            8299..8445
                     /locus_tag="VF516_28725"
     CDS             8299..8445
                     /locus_tag="VF516_28725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111763.1"
                     /translation="MSFRSPLSYFHNGFAKDLDAVVDFYEDRFHLNLSKQERSDLIAF
                     LRSL"
     gene            8453..8731
                     /locus_tag="VF516_28730"
     CDS             8453..8731
                     /locus_tag="VF516_28730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111764.1"
                     /translation="MTEAIEMTPPERPEPPRVDWPPERTIDRIWPVGEPADVRVVALR
                     DEQIHVARALVLAARLDRLDLGHHLGRYLLGPHRVGVVVVVGDAHRSR"
     gene            complement(8752..>10089)
                     /locus_tag="VF516_28735"
     CDS             complement(8752..>10089)
                     /locus_tag="VF516_28735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8111765.1"
                     /translation="LIIGGLRGATCAGPATAEPAGAAANPDLERELRRRSVELVRQDF
                     TFTQSVTLGGAKPMQLELLIPPGDNEHTIALWFEAEHGDASLRWLDPTGEVVTASRGR
                     RVEQRIARALAPGKHVVELRPSGSRVRALIGVHGPLVKQCAIDAARVTEQPADPAHGF
                     AWPYLLVAPRAAAPDARPPAGPTTVLVVPPNTGFATDDLELLRASTTCGVADQLAVAD
                     HLGTPILAPLFPRPALPSPPGNLYLHALSRASLEVRDPRVARVDLQLIAMLDHAGQRL
                     AAQGHPVQPRVLIIGHSAAGMFVSRFAVLHPDRVLAAAVSSPGGWPIAPVASAQDEAL
                     TYPVGIADVATLTGAPVDLAALRRVRFLFVLGDADTNDSVTDRDSFSAADEALIMRRF
                     GKTPVARWDAARRLYDAAGLPAQYRRYRGVGHEMSPAMKADVEAMFRAALGAR"
BASE COUNT         1783 a         3358 c         3263 g         1685 t
ORIGIN      
        1 ggacccggct ccggagatga agcgcctcag cgtctgctcg gctgcgggat ctgcggcgcc
       61 gagcgtcccc tcgatcgcat cgcctgcgcc gggtgcggtc agaccgagcc gaccaagctc
      121 gcggtgttcc agaccgagcg ccaccccgcg gtccgcatcg aggcctgcga cgcctgccag
      181 cgctacatca aggcgatcga cctgacgctc gatggccgcg cgatccccga agtggacgat
      241 ctgtgctcgc tgtcactcga cctgtgggcg acagaacaag gctacgagcg gctggaaccg
      301 tcgctcgccg gggtctgagc ccgcgcggcg ccgaggcgtg gagaggtgag ctcccggcgc
      361 tgcgcaaaaa agggacagga agtccctctg gatcgaccgc tgttccagat ccggggcagc
      421 gatccggatc gcagcgaccg ctcccggctc tgaacggccg atccctggac ggtttcaggt
      481 gcacccctgg cctgccgcag aaccggccaa tccggagtat cttccccttt ggtatcaata
      541 gcttgaatac gatctggcct ggcgcggcgg gtgcaaaagc gggaaggcat gaacacgcgc
      601 aaagttgtga tgctggttgc cctcctcgcg agcgttgacg gctgcgccac ggatggaagc
      661 gacgcgagcg acaccaacga tgccgcggac gacagcacca gggacggcaa catggaggac
      721 gagtcgacca tcgcgagcga cctcgacgcc gtgttcgccc tccatggtga cgaccatcac
      781 aaccggctgc ccaacaacgt cccggtcggg gatccgaccg gcgtgttcac gaccgtcagc
      841 tcgcacggct tcatcgacct caacaacgag ttcttccagg acctcggcac caacggccgg
      901 cgctgcgtca gctgccacgt gccgacctcg ggctggacga tcaccccgcg gcagctgcag
      961 accgtgttcg acgccaccga cggcggcgtg ttcgacgacg gcttcggcct gtcggcggtg
     1021 ttccgtacgg tcgacggcgc caatgcgccc aacgccgacg tctcgaccct ggccaagcgc
     1081 cgtcaggcct acagcctcct gctcaacaag ggcctgatcc ggatccacct cgcgatcccg
     1141 accgacgccg agttcgagct cgtcgcggtc gacgacccct accacttcgc gaccgccgac
     1201 aacatgtcgc tgtatcgccg gccgccgccg accaccaacc tcaagttcga cagcacgatc
     1261 atgtgggacg gtcgcgagaa cgcccccggc cagaccatcg cgcaggacct cgccacccag
     1321 gccaacgacg cgctgctcac ccacgcccag ggcaagggcc tgacccaggc gcagcgcacg
     1381 tcgatcgtca acttcgagac cgcgctcgcc tcggcgcaga tcttcgaccg caccgcgggc
     1441 gagctccacg cggccggcgc ccagggcggc cccgacgcca tcctcgccca gccgttccac
     1501 atcggcatca acgacaactt cggcgacccg gtcaccggcg cgccgttcaa cccggtggtg
     1561 ttcacgatct tcgaccgctg gaccaacatc ggcctgggca aggactgcga ccacgaccac
     1621 gaccacgacc gcgacgaccg ctgccgctgc gacagcgacg accgcggcca cggccacggc
     1681 tacagctacg gccgcgaccg tgactgcgac cgcgacgacg ggatcaacgc cgcgcgccgc
     1741 gcggtcgccc gcggccaggc gatcttcaac acccgcccga tcatcatcag cggcgtgtcg
     1801 ggcatcaacg acgaggccgc gttcggcaag ccggcctcgc tgaccggcac ctgcacgacc
     1861 tgccacgaca cccccaacag cggcaaccac tcggtcgccg cgccgctcaa catcggcctg
     1921 gccgacgagt cgcgccgcac cccggacatg ccgctctaca cgctgcgcaa caagaccacg
     1981 cacgagatcg tcaaggtcac cgaccccggc cgcgccctgg tcgacggcaa gtgggcccac
     2041 atcggccggt tcaagggccc gatcctccgc gacctcgccc cccgcgcccc gtacttccac
     2101 aacggtttcg ccaaggacct cgacgccgtc gtcgacttct acgaggaccg cttccacctc
     2161 aacctctcca agcaggagcg ctccgacctg atcgccttcc tccgctcgct ctgagtcttc
     2221 cgtccccccc ccaccgagag ccggcgttcc ccccgccggc tctcggcctt ttcggccagc
     2281 agacggatca cgtcgatcac gagggggagc aggactctga gatcattcct ctgcccatac
     2341 atgagaatga gattcaggga tggccgctgt tctcagactc atttacgggc ccctgttcgc
     2401 gcggctcggt ggtgcgatcc tggttgattg aagtggcaca cgtgcggggc cgacaaatca
     2461 gggaacccaa cgatcgaaca caccctcagg cgctcgtgaa gccaacacag gacggactgc
     2521 acgatccgca atttgccaga agatatgctc caagtggaca tctcgcccaa agcgtcacga
     2581 aaccactctc tcacgatgta gctactcccc ctcaacacca accgctctcg cttggcttgg
     2641 cggcttagcc ccaattctgc gggagtgcgc tcgatgaatc ggatgaacgc gatttccact
     2701 cgcagccaag cctgcgctag gtggcatgcg gtggcacgca cctggctgga tctttcccgt
     2761 cgagcgctgc ggtcaattct ctcgatcgaa ttcgatgcaa catcaaccct cgtggtcgct
     2821 gctggcgcga gcggctcggt cgccgtttca gacgtcgcgc aagggatgcc ggtcacgatg
     2881 cttgacggac cgcgtaatgt cgtcctcgtt gcgcacttcg gcccaagttc gcagcaggtt
     2941 ctcggcgcat cctgggatgg cactgcacgc gtgtgggacg caagttcacc atatcgaaag
     3001 tggaaactca ccgccgatca ccgacggctg cgacgtcgtt acgagcctgg agccagatcg
     3061 ccggttcctt gcagtaggct gtaagagcta tccgacccgg gtatgggaca ccgctcacga
     3121 taggcttctc gcagagctgc ccagcgtgac cccgaccggt ggcgatttcg agccagcata
     3181 tcccgcggtg tcttttgcag gggaccgcgc tgcgatagcc cgcggcaaca ccgtcgaagt
     3241 ctacgacatt cggtcccgta ggtttcgctg ggcttgtccg gctgtccggc tgtccggctg
     3301 tccggagtgt ccgctcaccg gacagtatga gccaactagc ggagagccgg acgcgcaact
     3361 aaccccggtc ggggatctcc cggattccgg acacccgttc gagattcgga cgatatccgg
     3421 acaacagagc tgcagagccc gggattcgcc ccccgatcac cgctcaccgc tcagcttctt
     3481 gaagtaatcc gcgctgatct ccctagctgg aagtgtcggg tatttctcga gcatcgttgt
     3541 tgactgaaac gggcgcaaga cggagacgtg ctcgtccggg ggaggcgaga agaacagcgg
     3601 ggaagcgtag cgttcgctat tctcttcggt ggatggcgtc tcgacagtgt gtcgggacgc
     3661 gcgcaggcga tcccccgtga gccgctcaag ataaagccca gcctgaatga ggcattggtc
     3721 ttcaccgatc tcgacgggga gaaaccgacc atccgcgttg caggcctgga ggccggggcc
     3781 ggtaggcggg gtcaacaagg tgacgatcga catatcgcaa tgcttgctct gccgcctcgc
     3841 cagggtgtca acagaatccg gcatcggagg ataatgaatc aggtggattc gccctggccg
     3901 acgctcgagc aacgtgagga gctccttcgg ggctacttgg aggccgacgg cgagcgctgc
     3961 gagcacgtcg acaacgagcc gattcaggtg ctcgtggagc tctaccagga acgcccagtc
     4021 gtccagcatc gtcgccggcc accgggtcgg ggccgtcaag gagacgtccc aggactcgag
     4081 gaggttggcg atccccgtgt cgagcgcatg ctcgctgccg taggcgccaa agccaaggtc
     4141 gagggggtcc gtcgccatgt acgcaagctt ctcctccgcg gggagtgcga acaagcgccg
     4201 gagcagcgcg tggaaacgac gtgtaaccgg ccgcgggatc gcatccccga cgagtagaca
     4261 gcggttgtcc tcgaattcgc ggactaccct cgacgcgagc tcggcccgct cgcagggggc
     4321 tccacgacag aaatcgccgc gcgatatcgc tacgaggggt aggatgtact ggctggctac
     4381 catgggtagt gtctcatcat cgcatagttc ccgttgaggg tcaacttcag tcaacgggtg
     4441 atccagtagt tgccgatcgc cagcgcatcg atccgggtcg atcggaaggt cgccagcgcg
     4501 tcgttcggag agcacacgat aggttcgctg tcattgaacg acgtattcag gacgaggggt
     4561 actccggtcc tgacgtggaa gtgctcgatc agctgatgga aaagcggatt gtgctcccgt
     4621 gtcaccacct gcactcgtgc cgtccggtcg atgtgcaata ccgcggggat ccggtcgagc
     4681 acatgcgacc tgactggaca ggcgaatagc atcgcctgat agctctgaga cggccgcccg
     4741 agctcgaacc aggcgtcagc atgttcctcg aggacgctag gcgcgaacgg gcgaaaatgc
     4801 tcccggtgct tgaccttgac gttgagtatc tcccggatgt tcttgtctct ggggtctgcc
     4861 agcagggagc gattgcccag tgctcgaggc ccgaactcca tcgctccctg gaaccacccc
     4921 accacgagac ctcgggcgat ggcatcggcg gctcgcgcgg cgatcccatc gacctggatc
     4981 gcccggatgt ccttggccgc gaggagggcg cggatctcct cgtccaagta ccgggggccc
     5041 tgatatgggt gggccatcac gtccgtcgtc cggggcccgg cgagcacgtg gtggtacaca
     5101 tggagcgcag cgcccactgc tgttccggcg tcgtggggtg cagcggggat atagaccttt
     5161 tcgaacggac ccttctcctt gacgacccag ttggacgtgc agttcaaggc gacgcctccg
     5221 gccatgcaaa gcgcatcgga ggggtgaagc ttgtaaactg cctgtgcgag cgccagcatg
     5281 gcctgatcgt tgatggcctg gagcgcagcc gccgtatcga tgtgtcgtgc gatgagtgga
     5341 tcttccgggc ggcgcctcgg tcccaggatg cgctcgagtg gcgagaagtc gtcgagccgg
     5401 aactccatca cttcggcgtc gatggagaac ccgtcgttct cgacgacgac gaaggacccg
     5461 aactgcggcg ctgcgtcttc ccgtcgaccg tagcccgcga ggcccatcac cttgcaggcg
     5521 tcatactcgc cgaagccgag gtacttgcac accttctccc agaggaagcc gagggagtgc
     5581 ggataactca ggtgtttcag atacgtcagc tcgcgccccg tcccggcggc caggaaggtg
     5641 gtcgcctgct ccccgatacc gtccacgatt agcaccgcag cgtcgtcgaa cccggaggga
     5701 taataagtcg aggccgcgtg cgcgaggtgg tgaggcaccc agtggaatgc cgcgtcgacc
     5761 tcgtgtgaca gcacctgtga taccgcgcct ggcacccggt gcagcgaagc caggaaggac
     5821 tcttccccgg ctgcatcgcc ccaatctccc cgtttgctct gcgggtcgag ctgaaaggtc
     5881 ttccgccgga gtgtgcgatc gaaggaataa cacaccgcat cgagctggcg ggcctcgatc
     5941 ccggcactct cgaggcagaa tcggatggct gcctctggga gctcgtgggc gttgtccaca
     6001 cgcgcgggct tgccgtgctt gacgcgattg aagcgctcct cctcggcggc cgcgaccacg
     6061 cggccgtcga cgatcaacgc cgccgaggat tcatgataaa cggagttgat gccgaggagg
     6121 aacatcgtgc gggtgcttgt catgatggaa atatcaacgt tgattcgtca aggttgctcg
     6181 atgaagctca ggaccaggca ccgcagagag ccgccgctct tcttgaactc cgacatgtcg
     6241 cacgtggtca cctggtaacc gcgctccgca agcgcaccac gcagcccggc cgtgcagttg
     6301 tccatgagca cggtatcacc gatgacgagg ttgttgcagg caaagtattc gttggcgtct
     6361 ttgtcgctga gggcgatcgg gtacttgacc atcgaggaca gccgcctgac cccgtcctca
     6421 tcgaatgcct cggggtaata gagcaacgtg tctgcgtcga tgaacccgag agccatggct
     6481 aggtggaaat accgatcatc acggatgtgg agatcgccga ggatgtggag gtccgggacg
     6541 atgcgcttca ggtgttcgat cgaccgggcg tcggaccgcc ggccgtgtcc tacaatggct
     6601 ctcctgtcgt gaaacaccac gtcgccgagc ccctcgaaga acacatcgtc gggcattcgc
     6661 acgatgttga acccccgctg cttgaaccaa tgggcgtagt gagcgctctc ccctcgacgc
     6721 tcctggttgc gaaaattgct cgggatgaag gtgcgatcga ccaccaggcc tccatcgccc
     6781 gcgaacgtca tgtcgggtaa tcccggcatc ggctcgatca tctccaccgt gtctcccaac
     6841 tccttcaaga catcaatgaa gtgatgccat tgctcgaacg caagcgcctt gttcaccttg
     6901 acggcaacgt tcatccaggg gttgatgctg tactcgatgt cgaaatatgt gggtgcgcac
     6961 atcagccgga tcatcgatgg tccttggttg ccaagagact gacaagtcgg cttcaagccc
     7021 gactcacgac tctcagtatc ctatcgagag cctttgtgca acgatcgtca cctatatgtc
     7081 aaaatgcaag taccagcgca gcattgtgct gcgcgttgac tccttgtgga ggactatgac
     7141 tgcaccctct ttttgaatgg gctgtatttg tgcgatgcaa ttgtatcgat ggtaaagcat
     7201 atccggagtt gtggcttccg ggaccacccg tgctggctac cgatctcgtc cggcaggatc
     7261 acgacgcatc acgccgccct gcgatagtcg acgtggggcc attcggcttc gagccatgga
     7321 agctgctgcg gctcggcgag tcgctggagg atccgtccgt gatcgaggac gaggcggacc
     7381 gcctcaccgg ggccgacggc acggtgctcg tgcgcagccc gcgggctccg gatcgacggc
     7441 aacgaccacc tcgagccggg agtgcagcca ccgcggcggt cgaatcagcc aacgcaaact
     7501 ttgcgttggc tgtaccgata tcccatcgat caccgagatg ggcccgcaat cggtctgaga
     7561 gattttcttg cagcccctgg cagtgatcga gtggccaaga gtgacgatcg tgaggcactg
     7621 caacagcgcg ctacagcgga gaccgcaaca ttctacgata gccacttttc tgacctggcg
     7681 taggtggcgc cgctggtcgc cgcagcggtc gtgtcgtcgc gcggcgagta cccgacgatc
     7741 acgctgaccc ggcgcagcag ccaggccgct gccggcaagc tcgacgccgc tgcgatcgac
     7801 atccagatgc cgagccccag caagcaggcg agggtgaatg gtcaggccga cgggtctgga
     7861 agtacctcga agatcggacg tcgcgggcgc agctgtcccg ccgacatcac ggtgaaggaa
     7921 catcccggat tctccgttcg ccaacgtcga tcggacttcc ccggcggccg agcactgttc
     7981 ggcctgacgg tggtcgggat ccgccttcca gaaggaggac ggttgtgtgt ccttcgtgga
     8041 acacttgccc cgcaatgaca ccgagacgct gattgcgttg tcggggcaga cgtgtgtcgc
     8101 gtatcgcgtg cgggcctggc cgctcggctc gcccaacccc gtcgacggcg gcagcgtgtc
     8161 gcgcgacgcg cggccggaag caactccggc tttgccagcg ctttgccaga accggcggga
     8221 tccaacggca ccgagcggac ctcagcggga ttcgaccgcg aagaagcact tgaaagggtt
     8281 gcagtgcgtc gacaacactt gagttttcga tcgccccttt cgtacttcca caacggtttc
     8341 gccaaggacc tcgacgccgt cgtcgacttc tacgaggacc gcttccacct caacctctcc
     8401 aagcaggagc gctccgacct gatcgccttc ctccgctcgc tgtgagcgcg tcgtgaccga
     8461 ggcgatcgag atgactccgc ccgagcgccc cgagccgccg cgggtcgact ggccgcccga
     8521 gcgaacgatc gaccggatct ggcccgtcgg ggagccggcc gatgtgcggg tggtggcgct
     8581 gcgcgacgag cagattcacg tcgcacgggc gctcgtcctc gccgctcgcc tcgaccgtct
     8641 cgacctcggc caccacctcg ggcgctatct gctgggcccg caccgtgtcg gtgtggtggt
     8701 cgtcgtcggg gacgcgcacc gctcgaggtg atcaggccgt gaccggcatg ctcaccgcgc
     8761 gccgagcgct gcgcggaaca tcgcctcgac gtcggccttc atcgcggggg acatctcgtg
     8821 gccgacaccg cggtagcgcc ggtattgcgc cggtagaccg gcggcgtcgt acagccggcg
     8881 ggcggcgtcc cagcgcgcga ccggggtctt gccgaaccgg cgcatgatca gcgcctcgtc
     8941 ggcggccgag aagctgtcgc gatcggtgac ggagtcgttg gtgtcggcgt cgccgagcac
     9001 gaacaggaag cggacgcggc gcagcgccgc gagatcgacc ggcgccccgg tgagcgtggc
     9061 gacgtccgcg atgccgaccg gatacgtcag cgcttcgtcc tgcgcgctgg cgaccggcgc
     9121 gatcggccag ccgccggggg agctgaccgc cgcggcgagg acgcgatcgg gatggagcac
     9181 ggcgaaccgg ctgacgaaca tccccgctgc cgagtggccg atgatcagga cgcgcggctg
     9241 caccgggtga ccctgcgccg cgaggcgctg gcccgcatgg tccagcatcg cgatcagctg
     9301 caggtcgacg cgcgcgacgc gggggtcgcg gacctcgagg ctcgcccggg acagggcgtg
     9361 caggtagagg ttgccgggcg gcgatggcag ggccggccgc ggaaacagcg gcgcgaggat
     9421 cggcgtgccg aggtgatcgg cgacggcgag ctggtcggcg acgccgcagg tggtcgatgc
     9481 gcgcagcagc tcgaggtcgt cggtcgcgaa tccggtgttg ggcggcacga cgagcacggt
     9541 ggtgggcccg gccggggggc gggcgtccgg cgcggcggct cgcggcgcga cgagcaggta
     9601 aggccacgcg aagccatgcg cgggatcggc cggttgttcg gtcacccgcg ctgcgtcgat
     9661 cgcgcactgc ttgaccagcg ggccgtggac gccgatcagg gcgcgcacgc ggctgccgct
     9721 cggccggagc tcgacgacgt gcttgcccgg tgcgagcgcg cgcgcgatgc gctgctcgac
     9781 ccggcgcccg cgcgaggctg tcacgacctc gccggtggga tcgagccatc gcagcgacgc
     9841 gtcgccgtgc tcggcctcga accacagggc gatcgtgtgc tcgttgtcgc cgggggggat
     9901 cagcagctcg agctgcatcg gcttcgcgcc gccgagggtg acgctctgcg tgaaggtgaa
     9961 gtcctgccgg acgagctcga cgctgcgccg gcggagctcg cgctccagat ccgggttcgc
    10021 cgccgcgccg gccggctccg cggtcgcagg gccggcgcac gtcgcgccgc gcagaccgcc
    10081 gatgatcag
//
LOCUS       DASVOR010001524         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_366289,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001524 DASVOR010000000
VERSION     DASVOR010001524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366289"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VF516_28740"
     CDS             complement(<1..248)
                     /locus_tag="VF516_28740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111766.1"
                     /translation="MSTTEAGRPTPGKPGNPDPGDGVSLQAVLEAAIDSTRRLDELSC
                     ARVLGTIADAVHAAHRSGQPIAALTPSAILVRPDGSVA"
     gene            617..1846
                     /locus_tag="VF516_28745"
     CDS             617..1846
                     /locus_tag="VF516_28745"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8111767.1"
                     /translation="MHHVDVELRRILRLVGARSLNTFGRGVLTFTVLWELFDRTNDPL
                     ALSAVGLAQVVPVVLLFVPAGTLVDRSDRRTLATAAAATTGLVGVGLAVISALHVPLV
                     VYFVLVIALLIVQGCATSIHAPAVASLVPLIIARDQLTRANRISSSFAELAQIIGPAV
                     AGLALKFVAAAWIYAALAITGLSAGALYLSLPRPRAVQAPTASARKDWRAGLRFIFRS
                     PLLLPALTLDMFAVLFAGVTALLPAVAHVLHLDSFDLGLLRAAQSAGAVTMAVIGGRL
                     PAWKRPGRVLLIVVALFGLATIGFGLSTSFPLSLALLFACGALDNISVVIRLTLEQMV
                     VPDAIRGRVAAVHFVFIGMSNELGATESGVAARLLGTMPSIVVGGALAVAVAGVVSAR
                     WRALARMPPLQELQPGG"
     gene            complement(1863..>2342)
                     /locus_tag="VF516_28750"
     CDS             complement(1863..>2342)
                     /locus_tag="VF516_28750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111768.1"
                     /translation="LLRGAADGKLEVIARARTGYNFLTRAANPLMVGVHVQYNLTDKL
                     AVISGFPSTQQLSIALSDGGMMGAPKPIDLALPLSVGYQATPLLYFQLDTRLASLGLS
                     NSSNAFIGWDTTPVALTAVYNVLHALDVQASIGTDLSNSPGDALSFLVGARYYAGQL"
BASE COUNT          277 a          811 c          886 g          368 t
ORIGIN      
        1 aacgcgacgc tgccgtcggg ccgcaccagg atcgccgacg gggtgagcgc ggcgatcggc
       61 tgaccggacc ggtgggcggc gtggacggcg tcggcgatcg tgcccaggac gcgcgcgcag
      121 ctgagctcgt cgagccgtcg cgtcgagtcg atcgccgcct cgaggaccgc ctggagcgac
      181 acgccgtccc cgggatccgg gttgcctggc ttgcctggcg tcggacgtcc agcctcggtc
      241 gtggacacgc cttatcctag cgtaggtgag cggggagcga tcgctccgcg cagcggcacg
      301 aggcgatgcg cgacgccggt cgtgaccggt tcgacatgcc ggctgtgcac ccggcgtaca
      361 tcgctgcgag cgggcgccgg cgcggcgcgc ggcgatccgc ggtgcaggag acgccgcgaa
      421 acgacgcggc cggagcggcg gctttcgccc gtcgctcgcg agtcagaacg cccacatagg
      481 cgcggccccc ggcatgccaa tcgatcccgt ctacagtagc atcccacgga acgtggccaa
      541 ccgcgaccaa gggccagacc tgcacccacc ggcccgagag ccgatccctg atgctcgcat
      601 cgttcgcgac ggcgccgtgc accatgtcga tgtcgagctg agaaggatcc ttcggctcgt
      661 cggcgcgcgg tcgctgaaca cgttcggccg cggcgtgctc acgttcaccg tgctgtggga
      721 gctgttcgac cgcacgaacg acccgctcgc gctgtccgcg gtcggcctgg cgcaggtcgt
      781 gccggtcgtg ctgctgttcg tgcccgccgg gacgctggtc gatcgctcgg accgccgcac
      841 gctggcgacg gccgcggcgg cgaccaccgg cctggtcggc gtggggctgg cggtgatcag
      901 cgcgctgcac gtgcccctcg tcgtgtactt cgtgctggtg atcgcgctgc tgatcgtcca
      961 gggctgcgcg acgtcgatcc acgcgccggc ggtcgcgtcg ctggtgccgc tgatcatcgc
     1021 gcgcgaccag ctgacccgcg ccaaccggat cagctcgagc ttcgcggagc tcgcgcagat
     1081 catcgggccg gcggtcgccg ggctcgcgct caagttcgtg gccgcggcgt ggatctacgc
     1141 cgcgctggcg atcaccgggc tgtcggccgg cgcgctgtac ctctcgctgc cgcggccgcg
     1201 cgcggtccag gcgccgacgg cgagcgcgcg caaggactgg cgcgccgggc tgcgcttcat
     1261 cttccgctcg ccgctgctgc tgcccgcgct cacgctcgac atgttcgcgg tgctgttcgc
     1321 gggagtcacc gcgctgctgc cggcggtggc gcacgtgctc cacctcgact cgttcgacct
     1381 cggcctgctg cgcgcggcgc agtccgccgg cgcggtgacg atggcggtga tcggcgggcg
     1441 gctgccggcg tggaagcggc ccggccgcgt gctgctgatc gtggtcgcgc tgttcgggct
     1501 ggcgaccatc gggttcggcc tgtcgacctc gttcccgctg tcgctcgcgc tgctgttcgc
     1561 gtgcggcgcg ctcgacaaca tctcggtggt gatccgcctg acgctggagc agatggtcgt
     1621 gcccgacgcg atccgcggcc gggtcgcggc ggtgcacttc gtgttcatcg ggatgtcgaa
     1681 cgagctcggt gccaccgagt ccggcgtcgc ggcccggctg ctcggcacga tgccgtcgat
     1741 cgtggtgggc ggggcgctcg cggtggccgt ggccggtgtg gtctcggcgc gctggcgcgc
     1801 gctggcgcgg atgccgccct tgcaggagct ccagcccggc ggatagtgcg cgccggcggc
     1861 gctcacagct ggccggcata gtagcgcgcc ccgaccagga agctcagggc gtcccccggc
     1921 gagttggaca ggtcggtgcc gatcgaggcc tggacgtcga gcgcgtggag cacgttgtag
     1981 accgcggtca gcgcgaccgg cgtcgtgtcc cagccgatga acgcgttcga cgagttgctg
     2041 aggcccagcg aggcgagccg ggtgtcgagc tggaagtaca ggaggggtgt ggcctggtag
     2101 ccgacgctga gcggcagcgc caggtcgatc ggctttggcg cgcccatcat accgccgtcg
     2161 gacagcgcga tgctcagctg ctgggtgctc gggaaccccg agatcaccgc gagcttgtcg
     2221 gtgaggttgt actggacatg gacgccgacc atcagcgggt tggcggccct ggtgaggaag
     2281 ttgtagccgg tgcgcgcgcg cgcgatcacc tcgagcttgc cgtccgccgc gccgcgcagc
     2341 ag
//
LOCUS       DASVOR010001525         1799 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_541708,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001525 DASVOR010000000
VERSION     DASVOR010001525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1799
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541708"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VF516_28755"
     CDS             complement(<1..272)
                     /locus_tag="VF516_28755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111769.1"
                     /translation="MRTNILCRTSYRIRSGVAIGSALALAAVTGHAAPAAAPPATTLM
                     VCSPGSPGSTDEARPRMDAFAGALSTRAGTRIAATYEPAEDACVAQL"
     gene            428..1381
                     /locus_tag="VF516_28760"
     CDS             428..1381
                     /locus_tag="VF516_28760"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8111770.1"
                     /translation="MTILLRTARRPFAAIVELSRDWLLPSRTMPRRSWPLAIGVAYVA
                     VICALGDLHTSNLAIGALCLLDAYNPNTRRFLGVFWPFIVTGALYESLRYYLDATTTG
                     RIHVAELYVLDRAWFGIGTHTLNEVFAVHHWVVADLAAGFAYLIYVAEYLALALLAFF
                     AGGVARAHTFARGFLVVNAMGFVTYRVDPAAPPWYVAAHGFGPPLLGSAPSPGAAARF
                     DALLGTEVFEDMYRHSVDVFGAIPSLHAAYPVMAAILVFRTRELGWARWPAAGYAALM
                     CFSAVYLQHHYVIDVLLGLSYGTVAVIAVLAWERRRAAPVT"
     gene            1383..>1799
                     /locus_tag="VF516_28765"
     CDS             1383..>1799
                     /locus_tag="VF516_28765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111771.1"
                     /translation="MPADRISHALAGVLRHRAIVLAVYLIAVPAAALLATRIPSQGAI
                     ERLTVATDPDYAATRAFQAIFPEAQPVLLVFEAADPWSPATLDRVRRATAALDAVPHV
                     SAFSVLDALRRAQPDADPATLRRLATGTELFRRQGLV"
BASE COUNT          230 a          679 c          596 g          294 t
ORIGIN      
        1 agctgcgcga cgcacgcgtc ctccgcaggc tcgtaggtcg ccgcgatccg cgtcccggcc
       61 cgggtcgaca gcgcgccggc aaacgcgtcc atccggggcc gcgcctcgtc ggtcgacccc
      121 ggcgatcccg gggagcagac catcagcgtg gttgccggtg gcgccgccgc gggcgcggcg
      181 tgcccggtga ccgcggcgag cgcgagcgca ctgccgatcg cgacgccgga gcggattcga
      241 taggatgtcc gacagagaat gttcgtccgc atgatgatga cttcactcga ggattgacat
      301 tggcaatggc gcctgcgtgc tccgcacatc gatcatgtgg cggcgacgca cacgctcatt
      361 gaggatgaaa tacagcgagt gattcacgac agcttgactc aaagcgacgc caccgctagc
      421 ccttgtcatg acaatcctgc tgcggaccgc gcgacggcca ttcgcagcaa tcgtcgagct
      481 gtcgcgcgac tggctgttgc cgtcccgcac gatgccgcgg cgatcatggc cgctcgcgat
      541 cggcgtggcc tacgtcgccg tgatctgcgc gctcggcgat ctgcacacca gcaacctggc
      601 gatcggcgcg ctgtgcctgc tcgacgccta caacccgaac acccggcggt tcctgggcgt
      661 gttctggccg ttcatcgtga ccggcgcgct ctacgagtcg ctgcgatact acctcgacgc
      721 gaccaccacc ggccggatcc acgtcgccga gctctatgtc ctcgatcgcg cgtggttcgg
      781 gattggcacg cacaccctga acgaggtatt cgcagtccat cactgggtcg tcgccgatct
      841 cgccgccggc ttcgcatacc tgatctacgt cgccgagtat ctcgcgctcg ccctgctggc
      901 gttcttcgcc ggcggcgtgg cgcgggcgca caccttcgca cgcggcttcc tggtggtgaa
      961 tgcgatgggc ttcgtgacct accgcgtgga ccccgccgcg ccgccgtggt acgtcgccgc
     1021 gcacggcttc ggcccgccgc tgctcggcag cgcgccgtcg cccggcgccg cggcccggtt
     1081 cgacgcactg ctcggcaccg aggtgttcga ggacatgtac cgccacagcg tcgacgtgtt
     1141 cggcgcgatc ccgtcgctcc acgccgcgta tccggtgatg gcggcgatcc tcgtgttccg
     1201 cacccgcgag ctcggctggg cgcgctggcc ggccgccggc tacgccgcgc tgatgtgctt
     1261 cagcgcggtg tacctccagc atcactacgt gatcgacgtg ctgctcggcc tgagctacgg
     1321 cacggtcgcg gtgatcgcgg tgctggcctg ggagcgccgg cgcgcggcgc cggtgacctg
     1381 acatgcccgc cgatcggatc tcccacgcac tcgccggcgt gctgcgccac cgcgcgatcg
     1441 tgctcgccgt ctacctgatc gcggtccccg ccgccgcgct gctcgccacc cggatcccga
     1501 gccagggcgc gatcgagcgc ctgaccgtgg cgaccgatcc ggactacgcg gcgacccgcg
     1561 cgttccaggc gatcttcccg gaggcgcagc cggtcctcct ggtattcgaa gccgccgatc
     1621 cgtggtcgcc ggcgacgctc gaccgcgtgc ggcgcgccac cgccgcgctc gacgccgtcc
     1681 cccacgtctc ggcgttctcg gtgctcgacg cgctccgccg ggcgcagccc gacgcggacc
     1741 ccgcgaccct gcgccggctc gccaccggga ccgagctgtt ccgccgccag ggcctggtc
//
LOCUS       DASVOR010001526         6089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_308079,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001526 DASVOR010000000
VERSION     DASVOR010001526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6089
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308079"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..940
                     /locus_tag="VF516_28770"
     CDS             <1..940
                     /locus_tag="VF516_28770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111772.1"
                     /translation="ARAQVMVRRTYPMLVAATAALELLLRSAVPAASAPPSSSLTHYK
                     PTNPAALAHFEAGNRAYKDARDTTRPLTDRVRDLKRAIDEYTAGQAIDGAAAFDYNIA
                     HCARLLVDNATAVAHLQRFLERAGPLDPELRQSVESEIAELDPSGAIRALLRPSSAPH
                     SARAAPPAAPRAAPVPDGSTAQGTAQQVPPSAPPATRTAPPPRTSRTWLHLGWGLTAA
                     GVIDGGVAAWLAFDAAGLDRDARDMTRTISSRVDLEHRADVRRNAAVVMGIGGGAAVI
                     LGIVTLLRPARADASAASSAWNLGVTRGAVAVIGRF"
     gene            940..3570
                     /locus_tag="VF516_28775"
     CDS             940..3570
                     /locus_tag="VF516_28775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111773.1"
                     /translation="MAIAAGTQTETLTFLRELGHGGMGTVYLAEDRQLGKRCAVKVLH
                     SHLAQDETARARFQNGVRAAAAIDHPAIVKTYAVEELPDGRPCCRMEYVEGPTLSEFC
                     KRRGEPLPLGLILQIIAPICEAFDHLHDLHIVHRDLKPDNVLLVERGGQLYPRVLDFG
                     IARRIDEPRLTGPGIAPGTAAYMAPEQVKGGAADRACDVYSLGVMTYWMATGGHLPHE
                     VSDGMMYFAQVNEPPVDPRRRFSGISERAASVMLTAIHPEPMRRPRSMGALALMLARC
                     VGGGGLQPDGTAILRTCAPSLLVIGNLDETLRAPSVFGARGPQAVMWKYDYGPVLGRG
                     GMAEVVRATLRGEGQFAAPRALKLILPELATTPEFEEWFHEEARIAAMLEHRNIVRVL
                     EHDRDPMGRLYLAMEFVEGVDLDKLRRTGPMPFAVTIHVLCEVLEALEFAHHLPPASP
                     LASPQEIIARSGARGIVHRDVSHHNVLVSWLADVKLSDFGIAKLRTATAAAGSKLIKG
                     KPGYMAPEQASAHDKLDGRADLWAIGVMLWELLTGRLLFEFDDFAATMYAVLWDDIPR
                     PGTVCQGVPADLEAITMRLLERDLTRRFQTARQVITALQACRSASREGRAELAHLLAE
                     RFPERALRVPAPPDPAAAADNHHPPDEPSPPGPCTFAVSTSESPAVPWQAPTTGHTHG
                     QVVGSPARRARRWPWIPAGVLSLAAASTLALVRRPDLRAVDATDATRPTSSPRTGSVP
                     APAIAPPSPSASNPPTPAPSLMTATIATRPPGARAWIEASSMPAIAALSPLTVHVMPG
                     TRVHIRAELEGFQAAWQDATIDHEGQTVEVTLVPLDGQLSPARTIRHSALVPGSAAVA
                     KPRRPARPPSPPSDDPGIIE"
     gene            3729..4997
                     /locus_tag="VF516_28780"
     CDS             3729..4997
                     /locus_tag="VF516_28780"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX8111774.1"
                     /translation="MPADIKPQRLGESFAHHSQVPDLQKAGMIMRLLYPAVLPVLSLA
                     ISCDRHWDDLQCEADSNCDLAAGGVCSPATTGHHWCAYPDPSCSSGYRYSDVQVGDGA
                     SGTCVAASSDGDGGAATSCIALPSTCGPGKNGDCCESPLVSGGAYLRSYDTVSDGYYS
                     DKYAPATISSVRLDKYEVTVGRFRAFLAEGLGTQAKHPDTGKGAHKSIPGSGWEASWN
                     SNLAPDTTTLATALKSTSCSSIGVNTWTDMPGRNENLPINCVTWYEAMAFCVWDGGYL
                     PSEAEWNYAAAGGSEQRAYPWSTPPGDLTIDISHASYDCMADGYSTCRLTDIMPVGST
                     PAGDGRWGQSDLAGNVYELLLDYGVPPYLTPCTDCANLQIYASQGGARIIRGGSYGGG
                     PDTLRTGFRLGLPPLQRDADVGFRCARPAP"
     gene            5245..5610
                     /locus_tag="VF516_28785"
     CDS             5245..5610
                     /locus_tag="VF516_28785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111775.1"
                     /translation="MTRNWLDDAHWVHLSSGDGYLSLSQADWDEYGTHTTASGPPRFM
                     HIGLAVTNLDDIIRRLDTKGIDYWAVDGPIGRQLYLNQPDGDAAIGMNVELVRYRGRR
                     RSVRSALIRAEIRRRPKRS"
BASE COUNT         1000 a         2101 c         2027 g          961 t
ORIGIN      
        1 cgcgcgggcg caggtgatgg tgaggcgtac gtatccaatg ctcgtggccg cgacggcggc
       61 gctggagctg ctgctccgat cggcggtacc cgctgcctcg gcgccaccgt ccagctcgct
      121 cacccactac aagccgacga acccggccgc gctcgcccat ttcgaggcgg ggaaccgcgc
      181 ctacaaggac gcacgcgata cgacccgtcc cctgaccgat cgcgtgcgcg atctcaagcg
      241 cgcgatcgat gagtacaccg ccggccaggc gatcgacggt gcggcggcat tcgactacaa
      301 catcgcgcac tgcgcgaggc tgctggtcga caacgcgacc gcagtcgctc acctgcagcg
      361 gttcctggag cgcgccggcc cgctcgaccc agagctccgg caatccgtcg agagcgagat
      421 cgccgagctg gacccgtcgg gcgcgatccg cgccctgctg cgtccgtcca gcgcccctca
      481 cagcgcgcgc gcggcgcctc cggcagcccc ccgagcggcc ccggtaccgg acgggagcac
      541 agcccagggc actgcgcagc aggttccacc gagcgcccca cctgcgaccc gcaccgcgcc
      601 cccgccgcgc acgtctcgca cgtggcttca cctcgggtgg ggattgaccg ccgccggagt
      661 catcgatgga ggggtcgccg cctggctggc gttcgacgca gcagggctcg atcgcgacgc
      721 cagggacatg acccgcacga tctccagccg cgtggatctg gaacaccggg ccgacgtgcg
      781 ccgcaatgcg gccgtcgtca tggggatcgg cggtggtgct gccgtgatcc tcggcatcgt
      841 gacgctcctg cgtcccgcac gtgcagatgc gagcgcggcg agcagcgcgt ggaacctcgg
      901 cgtcacccgc ggtgcggtcg cggtcatcgg tcgattctga tggcgatcgc ggccggtacc
      961 cagaccgaga cgctcacgtt cctccgcgag ctggggcacg gcggcatggg gacggtgtac
     1021 ctcgccgaag atcgccagct cggcaaacgc tgcgcggtca aggtgctgca ctcccacctg
     1081 gcgcaggacg agacggcacg agcgcgcttt cagaacggag tacgcgccgc ggcggcgatc
     1141 gatcaccccg ccatcgtcaa gacctacgcc gtcgaggagc taccggatgg tcggccatgc
     1201 tgccggatgg agtacgtcga gggcccgacg ctcagcgagt tctgcaagcg ccggggtgag
     1261 ccgcttccgc tcgggctcat tctccagatc atcgccccga tctgcgaggc gttcgatcat
     1321 ctacacgacc tccacatcgt gcaccgcgac ctcaaacccg acaacgtgct gctcgtcgag
     1381 cgcggcgggc agctctatcc gcgcgtgctc gacttcggga tcgccaggcg catcgacgag
     1441 ccgcgactca ccggcccggg gattgcgccg ggtaccgcgg cgtacatggc ccccgaacag
     1501 gtcaagggag gcgcggccga tcgagcctgt gacgtctact cgctcggcgt catgacgtac
     1561 tggatggcga cgggcggaca tctcccgcac gaggtctccg acgggatgat gtacttcgcc
     1621 caggtcaacg agccgccggt cgatccgcgg cgccggttct ccggtatctc cgaacgggcc
     1681 gcgagcgtga tgctgactgc gatccatccc gagccgatgc gacgcccccg atcgatggga
     1741 gcgcttgcac tgatgctggc ccgatgcgtc ggcggcggcg gcctccagcc cgacggcaca
     1801 gccatcctgc gcacatgcgc tcccagcttg ctggtgatcg ggaacctcga cgagaccctg
     1861 cgtgcgccga gcgtcttcgg cgcgcgcggc ccccaggcgg tgatgtggaa gtacgactac
     1921 ggtcccgtgc tcggtcgagg tgggatggcg gaagtcgtgc gtgcgacgct gcgcggcgaa
     1981 gggcagtttg cggcgccgcg cgcgctcaag ctcattttgc ccgaactcgc cacaacgccg
     2041 gagttcgagg aatggtttca tgaagaagcc cgcatcgcgg ccatgctgga gcatcgcaac
     2101 atcgtgcgcg tgctggaaca cgatcgcgac ccgatgggcc ggttgtatct cgcgatggag
     2161 ttcgtcgagg gggtcgatct cgacaagctt cgacgcacgg ggccgatgcc ctttgcagtc
     2221 acgatccatg tcctgtgtga agtcctcgaa gcgctggagt tcgcccacca cctcccgccg
     2281 gccagccccc tggcatctcc ccaggagatc atcgcgcgca gcggcgcgcg cggcatcgta
     2341 caccgtgacg tctctcatca taacgtgctg gtctcgtggc tggccgacgt caagctctcg
     2401 gacttcggga tcgcgaagct gcgcaccgca accgcagcgg ccggctccaa gctgatcaag
     2461 ggcaagcccg gctacatggc gcccgagcag gcatcggcgc acgacaagct cgacggacgc
     2521 gcggatctgt gggcgatcgg cgtgatgctg tgggagctcc tgaccggccg gctgctgttc
     2581 gagttcgacg acttcgccgc gacgatgtac gcggtgctct gggacgatat cccgcgcccc
     2641 ggcaccgtgt gtcagggtgt ccccgcggat ctggaagcga tcacgatgcg gctcctggag
     2701 cgcgatctga ccaggcgctt ccagaccgcg cgccaggtca tcaccgcgct gcaggcgtgt
     2761 cggagcgcgt cacgcgaggg ccgcgcggag ctcgctcacc tcctggccga acggtttccc
     2821 gagcgagcgc tgcgcgttcc cgcgcccccg gatccagcgg ccgcggccga caaccatcac
     2881 ccgccggacg agccatcacc gccggggcca tgcacgtttg cggtgtcgac ctcggaatcg
     2941 ccggcggtcc catggcaagc gccgaccacc ggccataccc acgggcaagt cgtcggctca
     3001 cccgctcgcc gcgcgcggcg ctggccatgg atcccggcgg gtgtgctctc gctcgctgcg
     3061 gcgagcaccc tcgcgctggt caggcgcccg gacctgcgag cggtggatgc cacggacgca
     3121 actcgtccca cgagctcgcc acgcaccgga tccgtgcccg cacccgccat cgcgccgcca
     3181 tcgccgagcg cttcgaaccc tcccacgccg gcaccctccc tcatgaccgc gacgatcgcc
     3241 acccggccac cgggcgccag agcctggatc gaagcatcga gcatgccggc gatcgcggcg
     3301 ctgtcaccgc tcacggttca cgtgatgccg ggaacccgcg ttcacattcg tgccgagctg
     3361 gaaggcttcc aggccgcatg gcaggacgcg acgatcgacc acgagggtca gaccgtcgaa
     3421 gtgacgctcg taccgctcga tggacagctc tctcccgcca ggacgatcag acactccgct
     3481 ctcgtgccag gctccgcagc ggtcgcgaag cccaggaggc cggcgcggcc cccgagcccc
     3541 cccagcgacg atccggggat catcgaatga gtccggggca gtggcattgg agcacatggc
     3601 ccggcgcttc ctcgtcggcg acgtgcatgg gcgctcccga acgatggctg gagcatggtt
     3661 gtaggaccgg agcgcgagcg ccggcgcacg ccgatctggg cgaccatcgc agtagccggc
     3721 caggccggtt gcctgcggac atcaaacccc agcgcctcgg cgagagcttc gcgcatcact
     3781 cgcaggtccc agatctgcag aaggcaggca tgatcatgcg tctcctttat ccggcggtac
     3841 tcccggtgtt gagcctggcg atctcgtgcg accgtcactg ggatgatctt caatgcgaag
     3901 cagattcgaa ttgcgatctg gccgccggag gtgtttgctc ccctgcgaca acgggccatc
     3961 attggtgcgc ctatccggat ccaagctgct cgagcggcta ccgttacagc gatgtccagg
     4021 tgggcgatgg tgcctctgga acctgtgtcg ccgcaagctc cgatggcgat ggcggagcag
     4081 ccacgagctg tatcgcgctg cccagcacct gcggtcccgg caagaatggc gattgctgcg
     4141 aaagtccact ggtcagcggc ggggcgtact tgcgcagcta tgacacggtg agcgatggat
     4201 actattcaga caaatatgcc cctgcaacca tcagcagcgt tcgtctggac aagtacgagg
     4261 tcaccgttgg acggtttcgc gcattcctgg ccgagggact cggcacgcag gcgaagcacc
     4321 ccgacacggg caagggagca cacaagagca ttcccggcag cggctgggag gcgagctgga
     4381 actcgaacct ggctcccgat acaaccacgc tcgcgaccgc actgaagtcc acttcttgca
     4441 gctcgatcgg cgtgaacaca tggacggaca tgcccgggcg caacgaaaat cttcccatca
     4501 actgcgtcac atggtacgag gcaatggcgt tttgtgtgtg ggatggtgga tatcttccat
     4561 cggaggccga gtggaactat gcggcggcag gcgggagcga acagcgtgcc tatccgtggt
     4621 cgacaccgcc gggcgatctc acgatcgaca tctcccacgc gagctacgat tgcatggccg
     4681 acggctacag cacctgcagg ctgaccgata tcatgccggt gggcagcacg ccggcgggcg
     4741 atggacgctg gggacaatcc gacctcgcag ggaacgtcta cgagctgttg ctcgattacg
     4801 gcgtgccacc ctacctcact ccatgcacgg actgcgccaa tctgcaaatt tacgcctccc
     4861 agggcggagc caggatcata cgcggtggct cgtacggtgg cggccccgac accctgcgga
     4921 cgggcttccg gctgggactt ccgccgctcc agcgcgatgc agatgtcggc tttcgctgcg
     4981 cgaggccggc cccttgatcg taacccctcc acggtcggcg tgtagacggc gaccctcgcg
     5041 gggtgcctgg gatcggtgcg gaggatccca tgccgagtcc gcaagtccat gcaccttatt
     5101 cgcgtcgccg ccgttggcgc gtcaccgaag cacgagcagc gctggacgcg ctcgcggcgt
     5161 cggggttgtc ggtgagcgtg ttcgccgagc aggaaggact cgacgaggag cggctgtacc
     5221 gctggcggcg ccgcttcgct cgtgatgacc aggaactggt tggacgacgc tcactgggtt
     5281 catctcagca gcggcgacgg atacttgtcg ctctcacaag ccgactggga cgagtacggc
     5341 acgcacacga ctgcgtcggg tccgccgcgg ttcatgcaca tcgggctcgc cgtcacgaat
     5401 ctcgacgaca tcattcgccg cctcgacacg aagggcatcg actactgggc agtcgacgga
     5461 ccgatcggtc ggcagctcta cctcaaccag cccgatggtg acgcagcgat cgggatgaac
     5521 gtcgagctcg tgcgctacag ggggaggcgt cgctcggtcc ggtcggctct cataagggcg
     5581 gagatccggc gcaggccgaa gcgctcatga gtgggaggtc cttctcgctc ggcgaacgcg
     5641 ctcaccgaca accccgacgc catgagcgtt cagcgcggct cgcacgtcgg gaacgcgcca
     5701 aaacccgcgc gtcgaatggt tcgatcggcg cgacgtgtgg aagctcgccg acagcttcgc
     5761 ggagttcatg gcgggcctga agccgctcga cgcggcgtct tgatcgctcg cggctctcca
     5821 ctgttcgcaa tgtcgtggat gcccgagggg gccgtgctgg ctaccatgcg catcccagcg
     5881 agaacctggc gggagctggt ggcgctgggt cgagcgcgag gtcgagatcc ggctcgctcg
     5941 cgactgacgt ccgcgctcag ccgtacagcg tcttggcgca gcgcgcggcg tagtcggctt
     6001 cgatctcctc gacggtcccc ggtaggtcgg ggtgcagcga cttgacgatc gcaccttgcc
     6061 gcggcagcgc gtctgacttc caggtctcc
//
LOCUS       DASVOR010001527         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_308092,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001527 DASVOR010000000
VERSION     DASVOR010001527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308092"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VF516_28790"
     CDS             complement(<1..595)
                     /locus_tag="VF516_28790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEX8111776.1"
                     /translation="MAFFQQPPRLANQFDDDPMLVSWIARFCPELAAELRELGDVAVE
                     YYGKQLADRRNEPVLTQWDAWGQRVDRIEVSPLWREAQALAARTGMVAAGYEARHGAH
                     ARTHQFAIVHVLGPSLDVYTCPLAMTDGAARTLLASGNQALIERYVPRLVSRDPAVMW
                     TSGQWMTERTGGSDVSQSETVARPDGDAGGWRLYGTKW"
     gene            complement(628..927)
                     /locus_tag="VF516_28795"
     CDS             complement(628..927)
                     /locus_tag="VF516_28795"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEX8111777.1"
                     /translation="MATKKLDTDEIARWLAQLPGWTVEAGKLHREYRFTNFVEAFGVM
                     TSVALVAERMDHHPEWFNVWNTVRVDLMTHDAGGITAKDFELAEAMERIAAGKAK"
     gene            1009..1746
                     /locus_tag="VF516_28800"
     CDS             1009..1746
                     /locus_tag="VF516_28800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEX8111778.1"
                     /translation="MNLLLVDPEELAGDGTCIVRDHRALHLRSVIGVRAGWHVRAGVV
                     GGGIGSAEVRADDGVAITLRLKLTEPAPLPLPIELVLAVPRPKVLSRAIETAAAFGIA
                     RIDLTNAWRVDKGYLTSPRLQPEALARAARTGAEQGGTTWLPDIQVHRRLMALLGERW
                     PGPPATKLIAHPGAPPIERAILGPPEPTVLAIGPEGGWIAREVETFIGRGFTPVSLGA
                     PILRVETAVAAGLAQVLLLHRLAEVTS"
     gene            complement(1754..>1990)
                     /locus_tag="VF516_28805"
     CDS             complement(1754..>1990)
                     /locus_tag="VF516_28805"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX8111779.1"
                     /translation="GAPSIRAGSGVASLRLAAAYRPRVVSGLVSGLHVPGESHFELLS
                     AFAPPDQLRRAIALAAQHGLSGHELGDACLILPG"
BASE COUNT          253 a          773 c          690 g          274 t
ORIGIN      
        1 accacttggt gccgtacagc cgccagccgc ccgcgtcgcc gtccggccgc gcgacggtct
       61 cggactgcga gacgtccgag ccgccggtgc gctcggtcat ccactgcccg ctggtccaca
      121 tcaccgccgg atcgcggctg accaggcgcg gcacgtagcg ctcgatcagc gcctggttgc
      181 cgctcgccag cagcgtgcgc gccgcgccgt cggtcatcgc cagcgggcag gtgtagacgt
      241 cgagcgaggg cccgagcacg tggacgatcg cgaactggtg ggttcgcgcg tgcgcgccgt
      301 gccgcgcctc gtaccccgcg gcgaccatgc cggtgcgcgc ggccagcgcc tgcgcctcgc
      361 gccacagcgg cgacacctcg atccggtcga cccgctggcc ccacgcgtcc cactgggtca
      421 gcaccggctc gttccggcga tcggcgagct gcttgccgta gtactcgacc gccacgtcgc
      481 cgagctcgcg cagctcggcc gcgagctcgg ggcagaaccg tgcgatccac gacaccagca
      541 tcgggtcgtc gtcgaactgg ttggcgaggc ggggaggctg ctgaaagaac gccatgcggg
      601 atgctacttc gttcgtcgca cccctgacta cttcgccttg ccggccgcga tccgctccat
      661 cgcctcggcg agctcgaagt ccttcgccgt gatcccgccg gcatcgtggg tcatgagatc
      721 gacccgcacc gtgttccaca cgttgaacca ctccgggtga tggtccatcc gctcggcgac
      781 cagcgcgacg ctggtcatca cgccgaacgc ctcgacgaag ttcgtgaacc ggtactcgcg
      841 gtgcaatttg cctgcctcca ccgtccaccc cgggagctgc gccagccagc gcgcgatctc
      901 gtccgtgtcg agcttcttgg tcgccatggc cgcttatacc gcggcggccc cgggcccggc
      961 acatccgggt tgccgccggc cgccgcgctg cgcgaacatc ccctcggaat gaatctcctg
     1021 ctcgtcgatc cggaggagct cgccggcgat ggtacctgta tcgtgcgcga tcatcgcgcg
     1081 ctccacctgc gcagcgtgat cggcgtgcgc gccggctggc acgtccgggc cggcgtggtc
     1141 ggcggcggca tcggcagcgc cgaggtcagg gccgacgacg gcgtcgcgat cacgctgcgg
     1201 ctcaagctca ccgagcccgc cccgctgccg ctgccgatcg agctcgtgct cgccgtgccg
     1261 cggcccaagg tgctgagccg cgcgatcgag accgccgcgg cgttcggcat cgcgcggatc
     1321 gacctcacca acgcgtggcg ggtcgacaag ggctacctga cgtcgccgcg gctccagccc
     1381 gaggcgctcg cccgcgccgc gcggaccggc gccgagcagg gcggcacgac ctggctcccc
     1441 gacatccagg tgcaccgccg gctgatggcg ctgctcgggg agcgctggcc cggcccgccc
     1501 gcgaccaagc tgatcgccca ccccggcgcc ccgccgatcg agcgcgcgat cctcggcccg
     1561 cccgagccca ccgtgctggc gatcggcccc gagggcggct ggatcgcgcg cgaggtcgag
     1621 accttcatcg gccgcggctt cacgccggtg tcgctcggcg cgccgatcct gcgggtcgag
     1681 accgcggtcg ccgccggcct cgcacaggtg ctgctcctgc accgcctggc cgaggtgacc
     1741 tcgtagccgc ccgtcatccg gggaggatca ggcaggcgtc gccgagctcg tggccggaca
     1801 gtccgtgctg cgcggcgagc gcaatcgcgc ggcgcagctg gtcgggtggc gcgaacgccg
     1861 acaggagctc gaagtggctc tcgcccggca cgtgcagccc gctgaccagg cccgacacca
     1921 cgcgcggccg gtacgccgcg gcgagccgca ggctcgcgac ccccgagccg gcgcggatcg
     1981 acggcgcgcc
//
LOCUS       DASVOR010001528         4022 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_424117,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001528 DASVOR010000000
VERSION     DASVOR010001528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424117"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..1271
                     /locus_tag="VF516_28810"
     CDS             228..1271
                     /locus_tag="VF516_28810"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEX8111780.1"
                     /translation="MKRWLGLLTSICVLGSFLGGLGGFTAVARAEPTRFVLVSHAPDS
                     DSWWNTIKNAIKQAGEDFGVTVDYRNPPSGDLADMARIIEQASARNYAGVITSIADID
                     VLKKPIAQVVAKKIPLVTINSGTTAQSEQLGAIMHVGQPEYDAGKAAGERAKAAGAKS
                     FLCVNHYATNPASFERCKGFADALGVDYKPATLDTGADPTTVESKVTAYLRKNPGTSA
                     VLALGPNSAHPTLKAIAKLGLKGKIFMATFDLSPEISAGIKDGTVAFAIDQQPYLQGY
                     IPVAVLAIMKQQNTQDLAKVMAALKASPKARARFDAYGLAPVYGARHISSGPGFVTKD
                     NIGKVEKFAGQYR"
     gene            1330..2373
                     /locus_tag="VF516_28815"
     CDS             1330..2373
                     /locus_tag="VF516_28815"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8111781.1"
                     /translation="MARPEATAIAGTALVFAIFGVTAGDSGMFALDGVISWSVVSGYL
                     GILSVGACLLMIAGEFDLSIGSMIGFTGMVAAIPPLHFGWPVWLAMTASLACALVLGF
                     VNGQLVVRTGLPSFIVTLAFLFVLRGLTLAFSVIVADRTIVSGIGDLAERDPVAGLLF
                     SGTFGDGLFGWLAHHHWIRTLDDGTPLVRGIPKVLLWWAAVAAASSFQLTRTRFGNWI
                     FAVGGDAGAAQNMGVPVRRVKVSLFMLTALCAWLFAMLQICDVGSAAADRGLQKEFEA
                     VIAAVIGGSLLTGGYGSVIGACFGAVIFGVVQIGITYTNLDSEWFRVFLGLMLLLAVL
                     FNNQVRRRVTQTR"
     gene            2376..3161
                     /locus_tag="VF516_28820"
     CDS             2376..3161
                     /locus_tag="VF516_28820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8111782.1"
                     /translation="MAPDPSSPYVLELDRVSKFFGTVIALNDVTVRLRRGEVHCLLGD
                     NGAGKSTLIKILAGVHPPSRGTYRVDGEPVAFASPRDALDRGIATVYQDLALVPLMSV
                     CRNFFLGREPTRTRWGVFRVFDRRLAATIATERLADLGITVRDAEQAVGTLSGGERQC
                     LAIARAIHFGARVLILDEPTAALGVKQSANVLRLVARARDKGISVIFITHNVHHAYPV
                     GDSFTLLGRGRSMGTFAKTEISRDEVLHMMAGGQEMQDLVTSA"
     gene            complement(3179..3778)
                     /locus_tag="VF516_28825"
     CDS             complement(3179..3778)
                     /locus_tag="VF516_28825"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX8111783.1"
                     /translation="MEQHRLWWGIPRLVLIEGVLALGALALLFALSLSGRSASAESDS
                     HPRPFTLTSPDFPECGSLPHRSKLDRFGCNGRNLAPTLRWTEVPPGTVSVALTMTDYD
                     APVAGGFHHWVVYNIPAHVDMVNGFSPFTQGTNSFGSPGYGGPCPPADGQIHHYIFTL
                     YALMTRENLKEGLTYEELIPLISPDVIGATVMIGTFVRR"
BASE COUNT          603 a         1401 c         1358 g          660 t
ORIGIN      
        1 agatcgatct gatgctggtg gcgccgccgg cgagcctcga gccgctgacg atcgcagtcg
       61 ccgagctcgg cgatgacgcc ggcctcgtcg gggccgcccg gctcgcgcgc gacggggtct
      121 cgatcctcgc atagcgccag cgcggcgcag gccgccgccg cttgcaatca ccgatcgagc
      181 tggatacggt cgcaacactc atgcctgtac tcgcctggag aacgccgatg aagagatggc
      241 tcgggctgct cacgtcgatc tgtgtccttg gctcgttcct tggtggtctc ggcgggttta
      301 ccgcggtggc gcgcgccgag ccgacccggt tcgtgctggt cagccacgcc ccggactcgg
      361 actcgtggtg gaacacgatc aagaacgcga tcaagcaggc gggggaagac ttcggcgtca
      421 ccgtcgacta ccgcaacccg ccgagcggcg atctggcgga catggcgcgc atcatcgagc
      481 aggcctcggc gcggaactac gccggcgtca tcacctcgat cgccgacatc gacgtcctga
      541 agaagccgat cgcccaggtc gtcgccaaga agatcccgct ggtcacgatc aactccggca
      601 ccaccgcgca gagcgagcaa ctcggcgcga tcatgcacgt cggacagccc gagtacgacg
      661 ccggcaaggc ggccggcgag cgcgccaagg ccgccggcgc gaagtcgttt ttgtgcgtga
      721 accactacgc gaccaacccg gcgtcgttcg agcgctgcaa gggcttcgcc gacgcgctcg
      781 gcgtcgacta caagccggcc acgctcgaca ccggcgccga cccgaccacg gtcgagagca
      841 aggtcaccgc ctacctgcgc aagaacccgg gcacgtcggc ggtgctggcg ctcggcccga
      901 actcggcaca cccgacgctc aaggcgatcg ccaagctcgg gctcaagggc aagatcttca
      961 tggccacctt cgatctgtcg ccggagatct cggccgggat caaggacggc accgtggcgt
     1021 tcgcgatcga ccagcagccg tacctccagg gctacatccc ggtggctgtc ctggcgatca
     1081 tgaagcagca gaacacgcag gatctcgcca aggtcatggc cgccctcaag gccagcccga
     1141 aggccagggc gcggttcgac gcgtacggcc tcgccccggt gtacggagcg cggcacatca
     1201 gctcgggccc cggcttcgtg accaaggaca acatcggcaa ggtcgagaag ttcgccgggc
     1261 aataccgctg agctgatgtc ccatcgctcc gacgaccggg tacgccagct ctctgcgctg
     1321 catcgggtca tggcgcggcc cgaggcgacc gcgatcgccg gcaccgccct ggtgttcgcg
     1381 atcttcgggg tgaccgccgg cgactcgggc atgttcgccc tcgacggggt gatcagctgg
     1441 tcggtggtct cgggctacct cggcatcctt tccgtcggcg cgtgcctgct catgatcgcc
     1501 ggcgagttcg acctctcgat cggctcgatg atcgggttca ccggcatggt cgccgccatc
     1561 ccgccgctgc acttcggctg gccggtgtgg ctggcgatga ccgcctcgct cgcctgcgcg
     1621 ctggtgctcg gcttcgtcaa cggccagctc gtcgtccgca ccgggctgcc gtcgttcatc
     1681 gtgacgctcg cgttcctgtt cgtgctgcgc gggctgacgc tcgcgttctc cgtcatcgtc
     1741 gccgatcgca cgatcgtcag cggcatcggc gacctggcgg agcgcgatcc ggtcgcgggc
     1801 ctcttgttct cggggacgtt cggcgacggc ctgttcggct ggctggcaca ccatcactgg
     1861 atccgcacgc tcgatgacgg cacgccgctc gtacgcggca ttcccaaggt gctgctgtgg
     1921 tgggccgcgg tcgccgcggc gtcttcgttc cagctcaccc ggacgcggtt cggcaactgg
     1981 atcttcgccg tcgggggcga cgccggcgcc gcccagaaca tgggggtccc ggtccggcgc
     2041 gtgaaggtgt cgctgttcat gctcaccgcg ctgtgcgcgt ggctgttcgc catgctgcag
     2101 atctgcgacg tcggctccgc ggcggcggac cgcggcctcc agaaggagtt cgaggcggtg
     2161 atcgccgcgg tcatcggcgg cagcctgctc accggcggct atggctcggt gatcggcgcc
     2221 tgcttcggcg ccgtgatctt cggcgtcgtg cagatcggca tcacctatac caacctcgac
     2281 tcggagtggt ttcgcgtgtt cctcggcctg atgctcctgc tcgccgtgct gttcaacaac
     2341 caggtgcgcc ggcgcgtcac gcagacgagg tgatcatggc cccggacccg tcgtcgccgt
     2401 acgtcctcga gctcgaccgc gtcagcaagt tcttcggcac cgtgatcgcg ctgaacgacg
     2461 tcaccgtgcg gctccggcgc ggcgaggtgc actgcctgct cggcgacaac ggcgccggga
     2521 agtccacgct gatcaagatc ctggccggcg tccacccgcc gagtcgcggc acctaccggg
     2581 tcgatggcga gcccgtggcc ttcgcgtcgc cgcgcgatgc gctcgatcgc ggcatcgcca
     2641 cggtctacca ggacctcgcg ctggtgccgc tgatgagcgt gtgccggaac ttcttcctcg
     2701 gccgcgagcc gacccgcacg cgctggggcg tgttccgggt gttcgaccgg cgcctcgcgg
     2761 cgacgatcgc gaccgagcgg ctcgccgacc tcgggatcac ggtgcgcgac gccgagcaag
     2821 cggtcgggac cctgtcgggc ggcgagcgcc agtgcctggc gatcgcacgc gcgatccatt
     2881 tcggcgcgcg cgtgctgatc ctcgacgagc ccaccgccgc cctcggcgtg aagcagagcg
     2941 cgaacgtgct ccggctcgtc gcccgggcgc gcgacaaggg catctcggtc atcttcatca
     3001 cgcacaacgt gcaccacgcc tatccggtcg gcgacagctt cacgctgcta ggccgcggcc
     3061 ggtcgatggg cacgttcgcg aagaccgaga tctcccgcga cgaggtcctg cacatgatgg
     3121 ccggcggaca ggagatgcag gatctcgtga ccagcgcgta gccggatccg cggggccatc
     3181 accggcgaac gaacgtgccg atcatgacgg tggctccgat cacgtcgggg ctgatcagcg
     3241 ggatcagctc ctcgtaggtc aggccctcct tcaggttctc tctcgtcatc agcgcataca
     3301 gcgtgaagat gtagtgatgg atctgcccgt ccgccggggg gcagggcccg ccatagccgg
     3361 gggatccgaa gctgttggtg ccctgggtga agggcgaaaa gccattcacc atgtcgacat
     3421 gcgccggaat attgtagacc acccagtgat ggaatccgcc cgcgaccggc gcgtcgtagt
     3481 ccgtcatcgt cagggcaacg ctcacggtgc ccggcggcac ttcggtccag cgcagcgtcg
     3541 gggcgaggtt gcgcccgttg cagccgaagc gatcgagctt gctgcggtgc ggcagggagc
     3601 cgcattccgg gaagtccggg ctggtgaggg tgaacggtct gggatggctg tccgactcag
     3661 cactggcact tctgccggac agtgagaggg cgaacagcaa cgccagcgca cccagcgcca
     3721 gcactccctc gatgagcacg aggcgcggga tcccccacca cagacgatgc tgctccatgg
     3781 ctcgcaacct ttctcccgaa cacacccgat acaatctctc gtacgttcgt tgccgcccgt
     3841 tacccatcga acaggtgcag gcgaaggtag gtcgcctccg ccgtgcggca tcctgtccct
     3901 gggcgatggc gcacgccctg tcgagccgcg cgtcgggtca gggagctcgg cgctggtcgc
     3961 gcgcagcggc cagcgcccgc gccttgtcga gccggaggcg tgcggccggc tgatcggcca
     4021 cg
//
LOCUS       DASVOR010001529         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_220338,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001529 DASVOR010000000
VERSION     DASVOR010001529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_220338"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VF516_28830"
     CDS             <1..708
                     /locus_tag="VF516_28830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111784.1"
                     /translation="IHGKRVEAFAGSPGSQDVWLDGERAVRWRRGDQAVSVDLITGVA
                     TPAAAAPPPLRVSYRTHTWEISRTEQAIVARERGKVAWQTRRPYSALLGAVYLADLAP
                     MVRVANIGVFGGWAELNLLDIDATGSLHGQAAFPVPAISALGHAVDAVGNTAIAVRMD
                     SSLRHDFIVGYAANALLMYVYPLPEIPRADPVGVAVAPDAVLVFHDGDTFTVLPELST
                     PPTAPGAPRPPSQNPTP"
     gene            782..1702
                     /locus_tag="VF516_28835"
     CDS             782..1702
                     /locus_tag="VF516_28835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111785.1"
                     /translation="MFKSLLAATATLSLVACVQQDDGTPSIDRSLPTADQVSIKVPKS
                     TTSARTADGDVLGQLAPYYVITLGVTSTFNGGTAWVLTLIHAIVQTPPTTIQGNVYTW
                     DPGSNALDPADYKLVVTANADGTFSYELSGKSKTQPNAQFEILIKGSADPRPGEAKGK
                     GEFTVDFDAGKRVNPIQNGDAKGSMDVNYDLAKAHIGLTLTSTDAQGNPAAADYSYTA
                     AADGGGDMTFGVDANVGGTAALETLTLHSRWLGTGAGRTDARITGGDLGIVQVTASEC
                     WSTSFGRVYYHDSGNFALTEGAESSCAIPQ"
BASE COUNT          294 a          703 c          597 g          254 t
ORIGIN      
        1 atccacggca agcgcgtcga ggcgttcgcc ggctcgcccg gcagccagga cgtgtggctc
       61 gacggcgagc gcgcggtgcg ctggcggcgc ggcgaccagg cggtgtcggt cgatctgatc
      121 accggcgtcg cgaccccggc cgccgccgcg ccgccgccgc tgcgcgtgag ctatcgcacg
      181 cacacctggg agatctcgcg caccgagcag gcgatcgtcg cgcgcgagcg cggcaaggtc
      241 gcgtggcaga cccgccgccc ctactccgcc ctgctcggcg cggtgtacct ggccgacctc
      301 gcgcccatgg tccgggtcgc caacatcggc gtgttcggcg gctgggccga gctgaacctg
      361 ctcgacatcg acgccaccgg ctcgctgcac ggccaggccg cgttcccggt gccggcgatc
      421 agtgcgctcg gccacgcggt cgacgcggtc ggcaacaccg cgatcgcggt ccgcatggac
      481 agctcgctgc gccacgactt catcgtcggc tacgccgcca acgcgctgct catgtacgtc
      541 tatccgctgc ccgagatccc gcgcgcggat ccggtcggcg tcgcggtcgc gcccgacgcc
      601 gtcctggtgt tccatgatgg cgatacgttc acagtgttgc ccgagctgtc gacgcctcct
      661 acggcgcccg gcgcgccgcg gccgccgtcg caaaatccaa caccgtaagc cctcgagatc
      721 gctcatcaca cgctggtacg gcggctgctc taggggtttg ccaacaagga gccgtgaccc
      781 catgttcaag agccttctcg ccgccaccgc caccctgagc ctcgtcgcct gcgtccagca
      841 ggacgacggc acacccagca tcgacaggtc gctgccgacc gccgatcagg tctcgatcaa
      901 ggtgccgaag agcaccacca gcgcgcgcac cgccgacggc gacgtgctcg gccagctcgc
      961 gccgtactac gtcatcaccc tcggcgtcac cagtacgttc aacggcggca ccgcctgggt
     1021 gctgacgctg atccacgcga tcgtccagac cccgccgacg acgatccagg gcaacgtcta
     1081 cacctgggac ccgggcagca acgcgctcga ccccgcggat tacaagctcg tggtcaccgc
     1141 gaacgccgac ggcacgttca gctacgagct gtcgggcaag tcgaagaccc agcccaacgc
     1201 gcagttcgag atcctgatca agggctcggc cgatccgcgc ccgggcgagg ccaagggcaa
     1261 gggcgagttc acggtcgact tcgacgccgg caagcgcgtc aacccgatcc agaacggcga
     1321 cgccaagggc tcgatggacg tgaactacga cctcgccaag gcccacatcg gcctcacgct
     1381 gacgtcgacc gacgcgcagg gcaatccggc cgcggccgac tactcgtaca ccgcggccgc
     1441 ggacggcggc ggcgacatga cgttcggcgt cgacgccaac gtcggcggca ccgcggcgct
     1501 ggagaccctg accctgcact cgcgctggct cggcaccggc gccggccgca ccgacgcccg
     1561 gatcaccggc ggcgacctcg gcatcgtgca ggtcaccgcg tccgagtgct ggagcacctc
     1621 gttcggccgc gtgtactacc acgactcggg caacttcgcc ctgaccgagg gcgccgagtc
     1681 ctcgtgtgcc atccctcagt gaacctcgcg tgtcaccgac gcgcacggcc ccctgcgggg
     1741 ccgcccgcgt tttcggcagt cagcgttgac tgaaaacttc gcccgcggta acacgccggc
     1801 ggaggacaca catggctacg ttgcccaagg atgcgcagga gctgttcg
//
LOCUS       DASVOR010001530         2657 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_336808,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001530 DASVOR010000000
VERSION     DASVOR010001530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2657
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336808"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="VF516_28840"
     CDS             complement(<1..565)
                     /locus_tag="VF516_28840"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111786.1"
                     /translation="MGSDREQDIGRTATEAASAAAAVGTAEELGATLGRYRLEHKLGA
                     GGMGVVHAAFDPVLERRIALKVLNSATATLEARDRLLREARAMARLTHPNVVTVYEVG
                     TATGRDFVAMELVHGETLAAWLRAGQRTPAAILDAFLAAGRGLAAAHAAGIVHRDFKP
                     DNVMVSADGSVRVLDFGLARPHGDADEI"
     gene            670..1422
                     /locus_tag="VF516_28845"
     CDS             670..1422
                     /locus_tag="VF516_28845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111787.1"
                     /translation="MRFALRLLIAPYLAAAAACGHAQTQAAVPPGTPPTKLVVPEIEK
                     YRFNSRLLADRIYVIGRSDRQIAYAHEPADEACGCYLVEIIVQDLSTGARVWKDGYDS
                     GEIDRDKPPDPAQLRSLEQVWRVRGAAWEKRIHELGIVREPPRPLQAIATAAPSLPRL
                     EISTEEVDRNSPEGYRHLTNYHIDAITATGATRIATAERPPESDLLEVRVPGYLEPRG
                     AGPAAVLILEYRRGWEGSPNVFHLRVVGADLH"
     gene            complement(1419..>2657)
                     /locus_tag="VF516_28850"
     CDS             complement(1419..>2657)
                     /locus_tag="VF516_28850"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HEX8111788.1"
                     /translation="SSVGHDPGKDWSNGVLREDSFLRKSDKTTIQTSGTAKLVFLKGA
                     AVTLKSASGTWVEVEGPVHAKERGKSPVAAGTGKGWIERDWTSMSLGVFKDLPVDDRT
                     ATYGALATTKEFAAGDVTKIILHRTESSTGASTLAAYTSRIASGSTIGAHYLIDETGA
                     VILVVPVNKVVSHVSGLNSHSIGIEHVGMPTALNAPSSATDTATLNGIRTAVAALAMS
                     PQLQKRIAAMTDKQLFRFAKDAVEPGKTRWFLYGDINAVQKRASYLLVQKLEAHFGLG
                     DADVFAHEAVAAKSPGEGENIKEFLTARAAYPGQVTRLATLVAADATLAADATLTRIV
                     ATEKSTVDALKVDATAAENAAVTSGKDAAATARQAARDAFYAKFWTRSTQLDDLVAFL
                     KASGSSKPTKLATMTGAWVF"
BASE COUNT          355 a          964 c          912 g          426 t
ORIGIN      
        1 cgatctcgtc ggcgtcgccg tgcggccgcg cgaggccgaa gtcgaggacg cgcacgctgc
       61 cgtcggcgct gaccatcacg ttgtcgggct tgaagtcgcg gtggacgatg cccgcggcgt
      121 gcgcggctgc gagcccgcgc ccggcggcga ggaacgcgtc gaggatcgcc gcgggtgtcc
      181 gctggcccgc gcgcagccac gcggcgagtg tctcgccgtg gaccagctcc atcgcgacga
      241 agtcgcggcc ggtggcggtg ccgacctcgt agacggtgac cacgttgggg tgggtgagcc
      301 gcgccatggc gcgggcctcg cgcagcagcc ggtccctggc ctcgagggtc gcggtcgcgc
      361 tgttcagcac cttgagcgcg atccggcgct cgaggaccgg atcgaacgcg gcgtggacga
      421 cgcccatccc gccggcgccg agcttgtgct cgagccggta ccgcccgagc gtcgcgccga
      481 gctcctccgc ggtgcccacc gcggccgccg cgctcgctgc ctcggtcgcg gtccgcccga
      541 tgtcctgctc gcggtcactc cccacgccgg catgataacg cacgggtcgg ggttccaccg
      601 cagcatgctg cacaggcgac tgcgcacgtt tgcgccctcc cgttgagctg ctagtgtgtc
      661 agcgcgagca tgcgcttcgc tctccggctc ctgatcgcgc cgtacctcgc cgccgccgcc
      721 gcgtgcggtc acgcgcagac gcaggccgcg gtcccgccgg gaacgcctcc gacaaagctg
      781 gtcgtccccg agatcgagaa gtaccgcttc aattcccggc tgctcgccga tcgcatctac
      841 gtcatcggtc gatccgatcg ccagatcgcg tacgcccacg agcccgccga cgaggcgtgt
      901 ggttgctacc tcgtcgagat catcgtccag gatctctcga ccggcgctcg ggtctggaag
      961 gacggctacg actccggcga gatcgaccgg gacaagccgc cggacccggc gcagctgcgc
     1021 agcctcgagc aggtgtggcg cgtgcggggc gccgcctggg agaagcgaat ccacgagctc
     1081 ggcatcgtgc gggagccccc gcgacccctc caggcgatcg ccacggccgc cccgtcgctc
     1141 cctcggctcg agatctccac cgaggaggtg gatcgcaact ctcccgaggg gtaccgccac
     1201 ctgacgaact atcatatcga cgcgatcacg gcgacaggag ccacccggat cgccacggcg
     1261 gaacggcccc ccgagtccga tctgctcgag gtccgtgtcc cgggatacct ggagccgcgc
     1321 ggtgcgggtc ccgccgccgt cttgatcctc gagtaccggc gcggctggga gggctcgccg
     1381 aacgtcttcc acctgcgcgt cgtcggcgcg gatctgcact agaacaccca ggctccggtc
     1441 atcgtcgcga gcttggtcgg cttgctggat ccggacgcct tgaggaacgc cacgaggtcg
     1501 tcgagctggg tcgagcgggt ccagaacttc gcgtagaacg cgtcgcgcgc cgcttgccgc
     1561 gcggtcgccg ccgcgtcctt gccggaggtg accgccgcgt tctcggccgc ggtggcatcg
     1621 accttcagcg catcgaccgt ggatttctcg gtcgcgacga tccgggtcag cgtcgcgtcg
     1681 gccgcgagcg tcgcgtcggc ggcgacgagc gtggcgagcc tggtgacctg gccggggtag
     1741 gccgcccgcg cggtgaggaa ctccttgatg ttctcgccct cgcccggaga cttcgccgcg
     1801 acggcctcgt gcgcgaacac atcggcatcg ccgagcccga agtgcgcctc gagcttctgg
     1861 acgagcaggt acgacgcgcg cttctgcacg gcgttgatat cgccgtagag gaaccaccgg
     1921 gtcttcccgg gctccacggc gtccttcgcg aaccggaaca gctgcttgtc ggtcatcgcg
     1981 gcgatccgct tctggagctg cggcgacatc gccagggcgg cgaccgccgt ccgtatgccg
     2041 ttgagcgtgg cggtgtcggt cgcactcgac ggcgcgttga gcgccgtcgg catcccgacg
     2101 tgctcgatcc cgatcgagtg cgagttgagc cccgacacgt gagacacgac cttgttcacc
     2161 gggaccacga ggatcacggc cccggtctcg tcgatcaggt agtgcgcacc gatcgtcgac
     2221 ccgctggcga tccggctcgt gtacgcggcg agcgtcgacg caccggtgct gctctcggtc
     2281 cggtgcagga tgatcttcgt gacgtcgccg gcggcgaatt ccttggtcgt cgccagggct
     2341 ccgtacgtcg cggtgcggtc gtcgacgggc aggtccttga acacgccgag cgacatcgag
     2401 gtccagtcgc gctcgatcca gcccttcccg gtgccggccg ccaccgggga cttgccccgc
     2461 tccttcgcgt gcacgggccc ctcgacctcc acccacgtcc cggacgcgct cttcagcgtg
     2521 accgcagcgc ccttcaggaa cacgagcttc gccgttcccg aggtctggat cgtggtcttg
     2581 tccgacttgc gcaggaagct gtcctcgcgc agcacgccat tggaccagtc cttgcccgga
     2641 tcgtggccca ccgagga
//
LOCUS       DASVOR010001531         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_394903,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001531 DASVOR010000000
VERSION     DASVOR010001531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394903"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /gene="dacB"
                     /locus_tag="VF516_28855"
     CDS             <1..1556
                     /gene="dacB"
                     /locus_tag="VF516_28855"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEX8111789.1"
                     /translation="GPGDLRPPREVVGRREEPLSPEEDTARQIEQVLRGPLRYGVTGL
                     FVADARTGEALFSVNADDPLNPASNVKMISTATALELLGPDFRYPTRVLGATPDGGAV
                     HGDVYLLGSYDPTLTVGDLDQLAAAIAARGITRIDGDIVVGPDPTRDGVYRAVIPIDV
                     RAGEPGALAIAAAPPGMDLIAITLAARTARAPGRPHLGYQVQPTTDAAGHPRLAVTIN
                     GTIGKGGATRLAVVTRERTATAGYTLRAALLAHGVDVGGELKTAELAAFVAASTAGPG
                     LPIELARHESARLADIITRVNKWSINWLADRVIMTAAALVKREPPSMELALSAMYDWL
                     ARHPGIPRSDLVVDTGSGLSYHTRITTQELVSIVRAAAGFTPGGDLAQSHAWLDSLSI
                     GGTDGTLASRFRAGDVRGRLRGKTGTLSTVIALSGVLEVDPQRPLAFSLVTNGDSPLS
                     PRYIRRAHEQLIGLLCHYLAVTRQPGAPTDEPPPPAPTAAPVPADADRSDLEDLEDPD
                     LDAEIAPTP"
BASE COUNT          242 a          736 c          574 g          231 t
ORIGIN      
        1 ccgggcccgg cgacctccgg ccgccgcgcg aggtcgtcgg ccgccgcgag gagccgctca
       61 gccccgagga ggacaccgcc cggcagatcg agcaggtcct gcgcggcccg ctgcgctacg
      121 gcgtcaccgg cctgttcgtc gccgacgccc ggaccggcga ggcgctgttc tcggtcaacg
      181 ccgacgaccc gctcaacccg gcgtccaacg tcaagatgat ctcgaccgcg accgcgctcg
      241 agctgctcgg cccggacttc cggtacccga cgcgcgtgct cggcgcgacc ccggacggcg
      301 gcgcggtgca cggcgacgtc tacctgctcg gcagctacga cccgaccctc acggtcggcg
      361 acctcgacca gctcgcggcg gcgatcgccg cgcgcggcat cacccggatc gacggcgaca
      421 tcgtggtcgg ccccgacccg acccgcgacg gcgtgtaccg cgcggtcatc ccgatcgacg
      481 tccgcgccgg cgagcccggc gccctggcga tcgcggcggc gccccccggc atggacctga
      541 tcgcgatcac cctggccgcg cgcaccgcca gggccccggg ccggccgcac ctcggctacc
      601 aggtccagcc caccaccgac gccgccggcc acccccggct cgccgtcacc atcaacggca
      661 cgatcggcaa gggcggcgcg acccgcctgg ccgtggtcac ccgcgagcgc accgcgaccg
      721 ccggctacac cctgcgcgcc gccctgctcg cccacggcgt cgacgtcggc ggcgagctca
      781 agaccgccga gctcgccgcg ttcgtcgccg cctcgaccgc gggccccggc ctgcccatcg
      841 agctcgcccg ccacgagtcg gcccggctcg ccgacatcat cacgcgggtc aacaagtgga
      901 gcatcaactg gctcgccgat cgcgtgatca tgaccgccgc cgcgctggtc aagcgcgagc
      961 cgccgtcgat ggagctcgcg ctgtcggcga tgtacgactg gctggcgcgc caccccggca
     1021 tccccaggtc cgacctcgtc gtcgacaccg gctccgggct gtcctaccac acgcggatca
     1081 ccacccagga gctggtgtcg atcgtccgcg ccgcggccgg cttcaccccg ggcggggatc
     1141 tcgcccagtc gcacgcctgg ctcgactcgc tgtcgatcgg cggcaccgac ggcaccctgg
     1201 ccagccggtt ccgcgccggc gacgtgcgcg gccggctccg cggcaagacc ggcacgctgt
     1261 cgaccgtgat cgcgctgtcc ggcgtgctcg aggtcgatcc ccagcgcccg ctggcgttct
     1321 cgctggtcac caacggcgac tcgccgctgt cgccgcgcta catccggcgc gcccacgagc
     1381 agctgatcgg cctgctctgc cactacctcg ccgtcacccg ccagccgggc gccccgaccg
     1441 acgagccgcc cccgcccgcg cccaccgcgg cccccgtgcc ggccgatgcc gaccggtcgg
     1501 atcttgaaga tctagaagat ccggatctcg acgccgagat cgccccgacc ccctgataga
     1561 gatgtcgcta cgtcctcgac atggcgttcg tgatcgtcga cgtcgacctg atcgttgacc
     1621 caaagcgttg aggttgacgg cgacgtctct cgattgatca caagtcggcg agctcctcgc
     1681 gggcgcacca acctgcagcg aaggcaacga atttctccat gccgcgctgg aggacttgcc
     1741 aaccgggcgc cccgttgttc ttgatgtgac cgccgagacc ggc
//
LOCUS       DASVOR010001532         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_277780,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001532 DASVOR010000000
VERSION     DASVOR010001532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277780"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="VF516_28860"
     CDS             complement(<1..1778)
                     /locus_tag="VF516_28860"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX8111790.1"
                     /translation="MSKRWLGLLLCGVWLTGGVAHAMITPADNPLDLGNVVVGQSTTA
                     DTSLSADVNTTNVTVVVHGGAQCAEFSIVTPTGPVDIAMGNHVTVTVKLAPTSPGSKS
                     CVIDAKQGSMVRASFTATGTAVVGAPAIRVDLAPTFTATEVGKSSTAQLKLTNSGTAP
                     LTINTTSTGLTSGTDFLVTGGPTAALAVGEQTTWDITCKPTTFGPRTDTFKIVSDAVD
                     SATGMPDSPHNISLSCTGNQGVLAIDQNPTTIQFNTVPRGTTQTQTFTLSNIGNVDVS
                     TIAITVDPANVGYTVDTPTLNTLAASGGKKTITVTFKPQSKTDGGPATIHFTGSWGST
                     PTPTPALSLDVAGIESSYGTMPAQPALDFGKFRYDTHPQQTFQLLNDGNATLAMMAPF
                     APDNGTQATEYKVELANGAVPVAQPATLVADDHVTVVVSPQVAHRIGVVSGHIDITPS
                     LGATLHVPVTGVATAAQVEFPATVSFGVVDLNEPPMMQTIAIKNTGDSPLDIMSITPS
                     VPGVSGASSGAFAIMLPSTPAQLAPDASLTIQVIYQPTVATAGASDVVHLNAQLAGSL
                     DLGLSIQITGDAAFFRAHGSGGCNAGG"
     gene            1958..>2226
                     /locus_tag="VF516_28865"
     CDS             1958..>2226
                     /locus_tag="VF516_28865"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8111791.1"
                     /translation="MTETKSGLQFEDTVVGTGASPAAGQTCVMHYTGWLWVNGAKGAK
                     FDSSVDRGQPFSFPLGQGRVIKGWDEGVATMKIGGKRTLLIPPHLG"
BASE COUNT          307 a          699 c          841 g          379 t
ORIGIN      
        1 ccgccggcgt tgcagccgcc gctgccgtgg gcccggaaga acgccgcgtc gccggtgatc
       61 tggatgctga gcccgaggtc gagcgagccg gcgagctgtg cgttcaggtg cacgacgtcg
      121 gacgcgccgg cggtggcgac cgtgggctga tagatgacct ggatcgtgag cgatgcatcg
      181 ggggcgagct gcgccggcgt gctcggcagc atgatcgcga acgcgccgct cgacgccccg
      241 ctgacgccgg ggacgctcgg tgtgatcgac atgatgtcga gcgggctgtc gccggtgttc
      301 ttgatcgcga tggtctgcat catcggcggc tcgttgagat cgaccacgcc gaagctcacc
      361 gtcgcgggga actcgacctg ggccgcggtg gccacgccgg tgaccggcac gtgcagcgtg
      421 gcgccgaggc tcggggtgat gtcgatgtgg ccgctgacca cgccgatgcg atgcgcgacc
      481 tgtgggctga ccaccaccgt gacgtggtca tcggcgacga gcgtcgccgg ctgcgcgacg
      541 ggcaccgcgc cgttggcgag ctcgaccttg tactcggtcg cctgggtgcc gttgtcgggc
      601 gcgaacggcg ccatcatggc gagcgtggcg ttgccatcgt tgagcagctg gaaggtctgc
      661 tgcgggtggg tgtcgtagcg gaacttaccg aagtcgagcg cgggctgggc cggcatcgtg
      721 ccgtagctcg attcgatccc ggcgacgtcg agcgacagcg ccggggtcgg ggtcggggtg
      781 ctgccccagc tcccggtgaa gtggatcgtc gccggcccgc cgtcggtctt gctctgcggc
      841 ttgaaggtga cggtgatcgt cttcttcccg ccgctggccg cgagcgtgtt caaggtgggg
      901 gtgtcgacgg tgtagccgac gttggccggg tcgaccgtga tcgcgatggt gctgacatcg
      961 acgttgccga tgttgctcag cgtgaacgtc tgggtctggg tcgtgccgcg cggcacggtg
     1021 ttgaactgga tcgtggtcgg attctgatcg atcgcgagca cgccctggtt gccggtgcag
     1081 ctgagcgaga tgttgtgcgg gctatcgggc atgcctgtgg cgctgtcgac cgcgtcggag
     1141 acgatcttga acgtgtcggt gcggggaccg aacgtcgtcg gcttgcacgt gatgtcccag
     1201 gtggtctgct caccgaccgc gagcgccgcg gtcgggccgc cggtcaccag gaagtccgtg
     1261 ccgctggtca ggcccgtgct cgtggtgttg atcgtcagcg gcgccgtccc ggagttggtc
     1321 agcttgagct gcgcggtgct ggacttgccg acctcggtgg cggtgaacgt cggcgccagg
     1381 tcgacgcgga tcgccggcgc ccccacgacg gcggtgccgg tcgcggtgaa gctggcacgg
     1441 accatcgagc cctgcttggc atcgatcacg cagctcttgc tgccgggcga ggtcggcgcg
     1501 agcttgaccg tcacggtcac gtggttgccc atggcgatgt cgaccggacc ggtgggcgtc
     1561 acgatggaga actcggcgca ctgggcgcca ccgtggacca cgacggtgac gttcgtggtg
     1621 ttcacgtcgg cgctcagcga cgtgtccgcg gtcgtggact ggccgaccac gacgttgccc
     1681 aggtcgagcg gattgtcggc cggggtgatc atggcgtgcg cgacccctcc ggtgagccag
     1741 accccgcata gcaacagccc aagccagcgc ttcgacatcg agtggtcact cccccgagcg
     1801 cgtcgcctgg agctccccgg cgacgggctc atgaagaatc ttacaatggg cgacccgacg
     1861 gcgaaagcaa acggatccgg gggccgccgg ccgaggtccg tgtgacctta cgcaccgcag
     1921 gccaccgctg gggtcggtct cgctgtatag atcggcgatg accgagacca agagcgggct
     1981 ccagttcgag gacaccgtcg tcgggaccgg ggccagcccc gccgccggcc agacctgcgt
     2041 gatgcactac accggctggc tgtgggtcaa cggggcgaag ggcgccaagt tcgacagctc
     2101 ggtcgaccgc gggcagccgt tctcgttccc gctcggccag ggccgcgtca tcaagggctg
     2161 ggacgagggc gtcgcgacga tgaagatcgg cggcaagcgg acgctcctga tcccgccgca
     2221 cctcgg
//
LOCUS       DASVOR010001533         1847 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_73933,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001533 DASVOR010000000
VERSION     DASVOR010001533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73933"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VF516_28870"
     CDS             <1..382
                     /locus_tag="VF516_28870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8111792.1"
                     /translation="LRAFRKSSIGNRVVVLRDGAEALDYFFCRGAHAGRPASEIVQVV
                     LLDLKLPKVDGLDVLRALRADERTRLVPIVILTSSAEDQDLVHGYGLGANSYVRKPVD
                     FTQFVEAVRQLGRYWLVINQAAPV"
     gene            complement(430..1509)
                     /locus_tag="VF516_28875"
     CDS             complement(430..1509)
                     /locus_tag="VF516_28875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111793.1"
                     /translation="MTYHEGERPIGRARTTRVLALAAAVLAAGAGAARAQSLPLPKLD
                     NVNFFFQAGSLIGSHDLVDDPSSNEKTFGELGWGFETSFDMLTSPDWDIELAVGYDQM
                     FLRSKFVNKYGLRGTIRNLPAISIYASSPNGLYAGFGTGLVSLNNVSAFLDGHRAFTV
                     GGDTFDLAGRIGVSKDLGDKVATKYSRANLFIELAYHARFLGNVTYGMGAPDTLPGSL
                     YFGGVVASIGAQVSLKPSASSFDDDVKAEVKRLKSQVAPPVTGALTTVTGCVGVPPAA
                     GYVPIDDYWDPVRCGSPSSVEANVAVLALLPDAAPDGGPPGLEICAYAATPPGWKIIG
                     ERWDPTRCGRPTAATMNIKRIRREP"
     gene            complement(1521..>1847)
                     /locus_tag="VF516_28880"
     CDS             complement(1521..>1847)
                     /locus_tag="VF516_28880"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEX8111794.1"
                     /translation="SARQVHISGQVAFNAAGELVGKGDLAAQTEQVYTNLGHALAAAG
                     ASLADVFKIVTYVVGIAPDKVATIRAARGKFLGDGPYPASTMVGVTGLVHPDLLIEVE
                     AIAAID"
BASE COUNT          259 a          642 c          659 g          287 t
ORIGIN      
        1 gcttcgcgcg ttccgcaaga gcagcatcgg gaaccgcgtc gtcgtgctgc gcgatggcgc
       61 cgaggcgctc gactacttct tctgccgcgg cgcccacgcc ggccgcccgg cgagcgagat
      121 cgtccaggtc gtgctgctcg atctcaagct ccccaaggtc gacgggctcg acgtgctgcg
      181 cgcgctgcgc gccgacgagc ggacccggct ggtcccgatc gtgatcctga cctcgtcggc
      241 cgaggaccag gacctggtcc acggctacgg cctgggcgcc aacagctacg tccgcaagcc
      301 ggtcgacttc acccagttcg tcgaggcggt ccgccagctc gggcggtact ggctggtgat
      361 caaccaggcc gcgccggtgt gacgccggtg tgacgccggt gtgacgccgg tgtgacgccg
      421 gtgtgacgat catggctccc gccggatccg cttgatgttc atcgtcgcgg cggtcggccg
      481 gccgcaccgc gtcgggtccc agcgctcgcc gatgatcttc cagcccggcg gcgtcgccgc
      541 gtaggcgcag atctccaggc cgggcggccc gccgtcgggc gccgcgtcgg ggagcagggc
      601 gagcacggcc acgttggcct cgacgctgga cggcgagccg cagcgcaccg gatcccagta
      661 gtcgtcgatc ggcacgtagc ccgcagccgg cggcaccccg acgcatccgg tgaccgtggt
      721 cagcgcgccg gtcaccggcg gtgcgacctg gctcttgagc cgcttgacct cggccttgac
      781 gtcgtcgtcg aacgagctcg cgctcggctt gagcgagacc tgggccccga tgctggccac
      841 cacgccgccg aagtacagcg agccgggcag ggtgtcgggc gcgcccatcc cgtaggtcac
      901 gttgcccagg aaccgcgcgt gataggcgag ctcgatgaac aggttcgccc gcgagtactt
      961 ggtcgcgacc ttgtcgccga ggtccttgga cacgccgatc cgcccggcga gatcgaaggt
     1021 gtcgccgccg accgtgaacg cgcggtgccc gtcgaggaac gccgacacgt tgttgagcga
     1081 caccaggccg gtgccgaacc cggcgtacag gccgttgggg ctgctggcgt agatcgagat
     1141 cgccggcagg ttgcggatcg tcccgcgcag cccgtacttg ttcacgaact tcgagcgcag
     1201 gaacatctgg tcgtagccga ctgcgagctc gatgtcccag tccgggctgg tcagcatgtc
     1261 gaacgaggtc tcgaaccccc agccgagctc gccgaacgtc ttctcgttcg agctcggatc
     1321 gtcgaccagg tcgtggctgc cgatcagcga gccggcctgg aagaagaagt tcacgttgtc
     1381 gagcttgggc agcggcaggc tctgcgcgcg agccgcgccg gcaccggcgg cgagcaccgc
     1441 ggcggcgagc gccagcacgc gcgtcgtccg cgcccggccg atcggtcgtt ccccctcgtg
     1501 atacgtcatg tccccctatg ctaatcgatc gcggcgatcg cctcgacttc gatgagcaag
     1561 tcggggtgga ccagcccggt cacgccgacc atggtcgacg cggggtaggg gccgtcgccc
     1621 aggaacttgc cgcgcgcggc gcggatcgtc gcgaccttgt cgggcgcaat gccgacgacg
     1681 tacgtgacga tcttgaacac atcggccagg ctcgccccgg cggcggcgag cgcgtggccg
     1741 aggttggtgt agacctgctc ggtctgggcg gcgaggtcgc ccttgccgac cagctcgccg
     1801 gcggcgttga atgcgacctg gccggagatg tggacctggc gggcgct
//
LOCUS       DASVOR010001534         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_394946,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001534 DASVOR010000000
VERSION     DASVOR010001534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394946"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="VF516_28885"
     CDS             <1..1710
                     /locus_tag="VF516_28885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin export ABC transporter
                     permease/ATPase subunit"
                     /protein_id="HEX8111795.1"
                     /translation="RLPARPIGAREFLAFAMRGRGRDLVRIALVGVAAGALTVATPLA
                     TGLVFDSIIPGAARGQLLYLALGLLVGAVASGLFEITRSLSLLRLEGRTGAALQAAVW
                     DRLIDLPVSFFRDQTAGDLADRAAGVDTIRHDLSANLVTMVLATGFGLVNAVLLFYLD
                     LALGLWAGLLFAVPVVVATAAAARLLGRHRDATQLQGRLAGLVLQLVRGITKLRVAGA
                     EVRAYARWAELLHRQRWLALRTRAPLQVFGAVYPIAATLLLYALILGHRDGTMTTGAF
                     IAFMSAFNIVLYSLLQTAGSTLAILHVVPTFRRLAPILAARSEIDERKSDPGELDGEI
                     EIAHVSFRYAPGTPYVLSDVSLHIRPGEFVAIVGPSGSGKSSLLRLLLGFERPTAGHV
                     YYSGQDLEHVDLRKIRRQIGVVLQTSQLMSGDIFTNVVGSSELGRDEAIEALRMAGFG
                     PDLAALPMGLHTLISEGGSTLSGGQRQRLLIARAIVKRPRILYFDEATSALDNETQAH
                     VSDSIEKLEATRVVVAHRLSTIIHADRIVVLDHGRVVQSGTYAELIAQPGVFAELARR
                     QLL"
     gene            1773..>2299
                     /locus_tag="VF516_28890"
     CDS             1773..>2299
                     /locus_tag="VF516_28890"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8111796.1"
                     /translation="MAAGLLAATPSSAGPPETAVPAPSAPEPGLHVLDAIRTTLARSP
                     DIATAEQELESSRGALEVASGRFDVVIDAQVGQRGDSAPAIPSAAGSATTTGDSTTYT
                     TDYELGATQELRWGLKVQPHVSVSRVGGSLIDPPYAESHLGVTVTQPLWRGRGTAATA
                     ARETAARIDTDAAALR"
BASE COUNT          292 a          918 c          760 g          329 t
ORIGIN      
        1 cgcctgccgg cccgcccgat cggcgcgcgc gagttcctcg cattcgccat gcgcggccgc
       61 ggccgcgatc tcgtgcggat cgcgctggtc ggcgtcgccg cgggggcgct caccgtcgcg
      121 acgccgctcg cgaccgggct cgtgttcgac tcgatcatcc ccggcgccgc gcgcggccag
      181 ctgctctacc tcgcgctcgg cctgctggtc ggcgccgttg cctccgggct gttcgagatc
      241 acgcgcagcc tgagcctgtt gcgcctggag ggccgcaccg gcgccgcgct gcaggcggcg
      301 gtctgggatc gcctgatcga tctgccggtc tcgttctttc gcgaccagac cgccggcgac
      361 ctcgccgatc gcgccgccgg cgtcgacacc atccgccacg atctgtcggc caacctggtc
      421 acgatggtgc tcgccaccgg cttcggcctg gtcaacgccg tcctgctgtt ctacctcgat
      481 ctcgccctgg gcctgtgggc cggcctgctg ttcgcggtcc cggtcgtcgt cgccaccgcc
      541 gccgcggcgc gcctcctcgg gcgacatcgc gacgcgacgc agctccaggg ccgcctcgcc
      601 ggcctggtgc tgcagctcgt gcggggcatc accaagctcc gcgtcgccgg cgccgaggtg
      661 cgcgcctacg cacgctgggc cgagctgctc caccgccagc gctggctcgc cctgcgcacg
      721 cgcgcccccc tgcaggtgtt cggcgccgtc tacccgatcg ccgccacgct gctgctgtac
      781 gcgctcatcc tcggccaccg cgacgggacc atgaccacgg gcgcgttcat cgcgttcatg
      841 agcgcgttca acatcgtgct ctacagcctg ctccagaccg ccggctccac gctcgcgatc
      901 ctgcacgtgg tccccacctt ccgccgcctc gcgcccatcc tcgccgcccg ctcggagatc
      961 gacgagcgca agtccgaccc cggagagctc gacggcgaga tcgagatcgc ccacgtctcg
     1021 ttccgctacg ctcccggcac gccgtacgtg ctgagcgacg tcagcctgca catccgcccc
     1081 ggcgagttcg tggcgatcgt cgggccgtcg ggcagcggga agtccagcct gctccggctg
     1141 ctgctcgggt tcgagcggcc caccgccggc cacgtgtact acagcgggca ggacctggag
     1201 cacgtcgacc tgcgcaagat ccggcgccag atcggcgtgg tgctgcagac cagccagctg
     1261 atgtccggcg acatcttcac caacgtcgtc ggctcctccg agctcggccg cgacgaggcg
     1321 atcgaggccc tgcgcatggc cggcttcggc cccgatctcg cggcgctgcc gatgggcctg
     1381 cacaccctga tcagcgaggg tggctccacg ctgtccggcg ggcagcgcca gcgcctgctg
     1441 atcgcccgcg cgatcgtcaa gcgcccgcgc atcctgtact tcgacgaggc caccagcgcg
     1501 ctcgacaacg agacccaggc gcacgtcagc gacagcatcg agaagctgga ggccacgcgc
     1561 gtggtggtcg cgcaccgcct cagcaccatc atccacgccg atcgcatcgt ggtgctcgat
     1621 cacgggcgcg tcgtgcagtc gggcacctac gccgagctga tcgcgcagcc gggcgtgttc
     1681 gccgagctcg cgcggcggca gctcctgtga ggaacgtgat gtcgaacctt cttgcgagac
     1741 caggacggag cgaatgggcc aggcggctcg tcgtggcggc cggcctgctc gccgccaccc
     1801 cgtcctcggc gggacctccg gagaccgcgg tcccggcgcc gagcgctccc gagccaggcc
     1861 tccacgtgct cgacgcgatc cgcaccacgc tcgcgcgcag cccggacatc gccaccgccg
     1921 agcaggagct cgaatcatcc cgcggcgcgc tggaggtcgc cagcggccgg ttcgacgtcg
     1981 tgatcgacgc acaggtcggc cagcgcggcg acagcgcacc cgcgatcccc agcgccgccg
     2041 gctcggcgac gacaaccggc gacagcacga cctacaccac cgactacgag ctcggcgcga
     2101 cccaggagct gcgctggggg ctgaaggtcc agccccacgt ctcggtcagc cgggtcggcg
     2161 gctcgctgat cgatccgcct tacgccgagt ctcacctcgg cgtcaccgtc acccagccgc
     2221 tgtggcgcgg gcgcggcacc gccgccaccg ccgcgcgcga gaccgccgcg cggatcgaca
     2281 ccgacgccgc cgcgctgcg
//
LOCUS       DASVOR010001535         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_191334,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001535 DASVOR010000000
VERSION     DASVOR010001535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191334"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..1125
                     /locus_tag="VF516_28895"
     CDS             220..1125
                     /locus_tag="VF516_28895"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8111797.1"
                     /translation="MELRHLRSILALAEELHFGRAALRLAITQPALSQQLTQLEADLD
                     VRLFDRHPRVTLTPVGAALVDHARRILAAVAEAEAETRSLANLTRRRIRLGYLEYWNP
                     PFLARAMRALRELDPPVVLEARNLYSAEVLAGLRDRSLEIGFVHLPVDDDRLTVRPLL
                     EGRWSLVMPRAHPLAHRPELTLSDLTGEPLVFFDRTVNPPLHAWLLGRFAEAKLTPRI
                     VYATTQPQIGVDLVREGVGAFFVASYVLRTLPRGLVMRPFSDLPPMRIGAAWRGDHRT
                     PALSALLAALPKFPVRDHDLLPVRT"
     gene            complement(1098..1526)
                     /locus_tag="VF516_28900"
     CDS             complement(1098..1526)
                     /locus_tag="VF516_28900"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8111798.1"
                     /translation="MARHPYVIRPDHRPASIPSWDTEGWELPVLQPHISGRVAFLIGP
                     ETASYGESVMGLVEQYHLGEMVGAATAGANGNIAQIAEPTGCSSVFTGLRVTRLDGAR
                     FHLIGIQPTIPATRTIAGIAAGRDEVLEKALAYVRTGNRS"
     gene            complement(1505..1891)
                     /locus_tag="VF516_28905"
     CDS             complement(1505..1891)
                     /locus_tag="VF516_28905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111799.1"
                     /translation="MAEDEALASGSPQFRRVRARAQFASGPGGSVSRLRIRRAGVEQD
                     VTVTRNDKTAQGSSLPSIAMLDDGTYYVDLIRASMPEIDAVMERLAAAPAVVFDMRGH
                     PNGNHRVLSHLLTRPDDSTTWLATHT"
     gene            complement(2700..3083)
                     /locus_tag="VF516_28910"
     CDS             complement(2700..3083)
                     /locus_tag="VF516_28910"
                     /inference="COORDINATES: protein motif:HMM:NF040024.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6210 family protein"
                     /protein_id="HEX8111800.1"
                     /translation="MLVVEYPSGVIYQNQVGGNVCHQGKLEGVLAPLGLESNSVIQIM
                     QCSYGMQGITAETADIIDSILASDSEGKYVKVDRGRLEECLEAWVHVIVESPSSADPM
                     DTPEFFGGWCGFGRARAVLTWPNSD"
     gene            complement(3083..3334)
                     /locus_tag="VF516_28915"
     CDS             complement(3083..3334)
                     /locus_tag="VF516_28915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111801.1"
                     /translation="MTRYQDAASGANPVTSSWRFDSLGQMLELDEPGSAPQFNTYSNW
                     GELLQTVRYVTSTPSDGSGSDGGPIVDTPPPGYIRCQMQ"
BASE COUNT          588 a         1397 c         1099 g          623 t
ORIGIN      
        1 ctgccgccac cggcagcgcc gcggcccgcg cgccctgcgc gtcggaccac gcccggttcc
       61 tgaccgacgg agtcgcctgc accgctccgg cgcgagccgc gcgcgccacc tgcgtcgctt
      121 cgagcacgcc gccactgtgc cgctcgcccg gtccgcctgg caaatcaccc ccgctacacc
      181 gccgataagt ttcactgatg atcggctaga ctccctcccg tggagctccg ccacctccgc
      241 tcgatcctcg cgctcgccga ggagctgcac ttcggccgcg ccgccctgcg cctcgccatc
      301 acccagcccg cgctgagcca gcagctcacc cagctcgagg ccgacctcga cgtccggctg
      361 ttcgaccgcc acccccgcgt caccctcacc ccggtcggcg ccgccctcgt cgatcacgcc
      421 cgccgcatcc tcgccgccgt cgcggaggcc gaggccgaga cccgctcgct cgccaacctc
      481 acccgccgcc gcattcgcct gggttatctc gagtactgga acccgccgtt cctcgcccgc
      541 gccatgcgcg ccctgcgcga gctcgacccg ccggtcgtcc tcgaggcccg caacctctac
      601 tccgccgagg tcctcgccgg cctgcgcgac cgctcgctcg agatcggctt cgtccacctc
      661 ccggtcgacg acgaccgcct caccgtccgc cccctcctcg aaggccgctg gtcgctcgtc
      721 atgccccgcg cccaccccct cgcccaccgc cccgagctca cgctctccga cctcaccggc
      781 gagcccctcg tgttcttcga ccgcaccgtc aatccgccgc tccacgcctg gctgctcggc
      841 cggttcgccg aggccaagct caccccgcgc atcgtctacg ccaccaccca gccccagatc
      901 ggcgtcgacc tggtccgcga gggcgtcggc gccttcttcg tcgcctccta cgtcctgcgc
      961 actctacccc gcggcctcgt catgcgcccg ttctccgatc tgccgccgat gcggatcggc
     1021 gccgcctggc gcggcgacca ccgcaccccg gcgctctccg ccctgctcgc cgccctgccg
     1081 aagttcccgg tacgggatca cgatctgttg cctgtgcgta cataggcgag cgccttctcc
     1141 agcacctcat cgcggcccgc agcgatgccg gcgatcgtgc gggtcgccgg gatcgtcggc
     1201 tggatgccga tcaggtggaa gcgtgcccca tcgagccggg tgacccgtag accggtgaag
     1261 accgatgaac agccggtcgg ctcggcgatc tgcgcgatgt ttccgttcgc acccgccgtg
     1321 gccgcgccga ccatctcgcc gagatgatac tgctccacca gccccatcac cgactcgccg
     1381 tagctcgcgg tctcggggcc gatgagaaat gcgacgcgtc cggagatgtg aggctggagc
     1441 acaggcagtt cccatccctc ggtgtcccag ctcggtatcg aggccggccg gtgatcgggg
     1501 cgaatcacgt atgggtggcg agccacgtgg tcgagtcgtc cgggcgcgtg agcaggtgcg
     1561 acaggacacg gtggttgccg ttcggatggc cgcgcatgtc gaacaccacg gcgggcgctg
     1621 cggcgagtcg ctccatcacc gcgtcgatct ccggcatgct cgcccttatc agatcgacgt
     1681 aatacgtacc gtcatcgagc atggcaatcg aaggaagcga cgatccctgg gcggtcttgt
     1741 cgttccgcgt gacggtgacg tcctgctcga cgccagcgcg tcgaatgcgc aatctcgaca
     1801 cggatccacc cgggccactg gcaaactgcg cccgtgcgcg tacccgccgg aactgcggcg
     1861 agccgctggc gagcgcttcg tcctccgcca tctgttggcc gtctgcttcg acccggtcgc
     1921 ccctcctccc tctgatccac ccgacagcca cggcaggccg agagagagca agccccgggc
     1981 cgagtcggaa caccgccaag tactcggggt tgcaacgccg gctacggtac gctcctccgt
     2041 gctgtgttcc gaggcatgtt ctcgtgtgtt ccggagtatt gtatcagcac gaaaccaccg
     2101 tactttaaca gaacacaggc ggctgttccg gtgattccgc cgcaacacgt agatgctcga
     2161 tgtatcgatt gtactacccg ttacacgtca cagcgacggt gacggtagcc cagatggcct
     2221 ggtgccccgc ggcctcctca tgcgcccgtt cgccgacctc cccggctgcg gagtgacgaa
     2281 ggtacctcgt cagacagcgt cgccggcatc gtgctcgtgc tgcaggcgcg gtcgctcgag
     2341 gaccaggcgc gcgcgctgtg cccgaccggg caggagtgcc acgacctgac ggcgagccag
     2401 aagagcgagc gcgccgtgac gcgcgcgaac atcacgacca tcgtcggcgg cccggcatcg
     2461 tcgcgatcgg cgccggcgtc gtgctgtggt tcgccgtcgg tgcgtaccgc gggccgctcc
     2521 acgagggcag aacaggcgcg gtgtacgtcg gcgagcacga gcggctcggc cgtaaagcga
     2581 cgatcaaggt gctcctgcgc gggtcctcga cgcagcgacc gcgaccgtga tcgcgaagct
     2641 cgacgcgctc gactgccaag gtttccagca gagcgcgcac tcatctgatg acacacccgt
     2701 caatcgctat tcggccatgt caacaccgct cgcgcgcggc caaatccgca ccaccccccg
     2761 aagaactcgg gtgtatccat cggatccgcc gagctcggcg actccacgat cacatgcacc
     2821 catgcttcca aacattcctc aagacgccca cgatcgacct tcacatactt accttcggag
     2881 tcagacgcca gaatcgaatc aatgatgtcg gcggtttcag cggtaatgcc ctgcattcca
     2941 taggaacact gcatgatttg aatgacactg ttcgactcca agccgagcgg cgcaagcacc
     3001 ccttcaagtt taccctggtg gcacacgttc ccacctactt gattttgata gatcactcca
     3061 ctgggatact caaccaccaa catcactgca tctgacatcg aatataaccg ggtggtggcg
     3121 tatcgacgat gggtccccca tccgagcccg agccatcgga cggtgtcgag gtcacatacc
     3181 gcaccgtctg gagcagctcg ccccagttgc tgtacgtgtt gaactgcggc gcgctgccgg
     3241 gttcgtcgag ctccagcatc tggccgagcg aatcgaatcg ccaggaggag gtcaccggat
     3301 tcgcgcccga ggccgcgtcc tgatagcgcg tcatgcccgg tgcgatggcc cagccggtcg
     3361 tagctgaaca tcgcgtgctc caggcgcgcg gtcccttgcc aggtcgaccg ggtgaggacc
     3421 ctgccgatcg ccgtggcgta gctgaacttc ttgacgccgg attgcggcga gccaccgagc
     3481 agcgacgccg cgtcctagcg cacggcgcga cgcgggcgtt tcgagagccc gaccacgtac
     3541 gccatcccga cgactgtctc accgtgcctg tccctttcgg gaacagcatt aaatgagccg
     3601 ccgcgacaat gcatcaggcc tgcgcgacgc ggcggtcgaa ttgacgggtc gtcgcgattt
     3661 tcggatcaac ccgtcatgac gggtcgttgc gattttcgga tcaaccc
//
LOCUS       DASVOR010001536         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_104128,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001536 DASVOR010000000
VERSION     DASVOR010001536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104128"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1246)
                     /locus_tag="VF516_28920"
     CDS             complement(<1..1246)
                     /locus_tag="VF516_28920"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEX8111802.1"
                     /translation="MTAPAVPVRNRHATESIGIARPATSPSASSARGATDPDPSVRVA
                     PPPTSGPIRTEPGGGNAWTDALQAAALIVGVLFIVFAVYNWLAFPPRARGPVCIHDLC
                     LIALGSGIYALCRARRSLSLTAVHVGAAILSISVLSNILLAALLGSNPMFTHYVAVLI
                     IASAGSVMLARWAIAIAGVEVVAWAVVAQRLLSGTELRLHAFVILSTLAVAAIVQIGR
                     SKARARIQELRAGDARREQALQQALAEADEARRGLDRKVEERTAALRDELEERARLEE
                     QLRHAQKLEAVGRLAGGIAHDFNNLLTVIRMSLVTVGSAALSDEMREALSDASDATDR
                     AAGLTHDLLAFARKQTLQRTTVAVGDILGGVERMIRRVAEASIRLEVRAAPEVGEVVA
                     DRHQIEQVLLNLAINACDAMGNR"
BASE COUNT          207 a          569 c          542 g          196 t
ORIGIN      
        1 cgcggttgcc catcgcgtcg cacgcgttga tcgccaggtt gagcagcacc tgctcgatct
       61 ggtggcggtc ggcgaccacc tcgccgacct cgggcgcggc ccggacctcg agccggatcg
      121 acgcctcggc gacccgccgg atcatgcgct cgacgccgcc caggatgtcg ccgaccgcga
      181 ccgtcgtgcg ctgcagcgtc tgcttgcgcg cgaacgcgag caggtcgtgg gtcagccccg
      241 cggcgcggtc ggtcgcgtcg ctggcgtcgc tcagcgcctc gcgcatctcg tccgagagcg
      301 cggcgctgcc gacggtgacc aggctcatcc ggatcacggt gagcaggttg ttgaagtcgt
      361 gcgcgatccc gccggcgagc cggccgaccg cctccagctt ctgggcgtgg cgcagctgct
      421 cctcgagccg cgcccgctcc tccagctcgt cgcgcagcgc cgccgtgcgc tcctcgacct
      481 tgcgatccag cccccgccgc gcctcgtcgg cctccgccag cgcctgctgc agcgcttgct
      541 cccgccgcgc gtcccccgcc cgcagctcct ggatccgcgc gcgcgccttc gaccggccga
      601 tctgcacgat cgccgccacc gcgagcgtgc tcaggatgac gaacgcgtgg agccgcagct
      661 cggtgcccga cagcaggcgc tgcgcgacca ccgcccaggc cacgacctcg acccccgcga
      721 tcgcgatcgc ccagcgcgcc agcatcacgc tgccggccga cgcgatgatc agcacggcga
      781 cgtagtgcgt gaacatcggg ttcgagccca gcagcgcggc gagcaggatg ttgctcagca
      841 cgctgatcga caggatcgcc gcgccgacgt gcacggcggt cagcgacagc gagcgccgcg
      901 cgcggcagag cgcgtagatc ccggagccga gcgcgatcag gcagaggtcg tggatgcaca
      961 ccgggccgcg ggcgcggggc gggaacgcga gccagttgta gaccgcgaac acgatgaaca
     1021 gcacgccgac gatcagggcc gccgcctgca gggcgtcggt ccaggcgttg ccaccgccgg
     1081 gctccgtccg gatcggcccc gacgtcggcg gcggcgcgac gcggaccgac ggatccggat
     1141 cggtggcgcc gcgcgcggag gacgcgctcg gggacgtggc cggcctcgcg atgccgatcg
     1201 actcggtcgc gtgccggttc cggacgggta cggcgggggc ggtcatccgt gcgggggcat
     1261 cagcctactg ccgccagcag cggctgcgaa cgcctcacag tgcagcggcc cccccccgca
     1321 ccccgggtat cccgtacgat gatgtgatga cggatgggtc gagcgagcac gccggctggg
     1381 tgatccgcgg cgtgcgtgta ccgggtctcc tgtacggcac cgcgtggaaa gaggaggcaa
     1441 ccgagtcgct ggtgctcggt gcgctcaccg ccgggtttcg cggcatcgac accgcgaacc
     1501 agcgcaagca ctac
//
LOCUS       DASVOR010001537         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_249811,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001537 DASVOR010000000
VERSION     DASVOR010001537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249811"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..337)
                     /locus_tag="VF516_28925"
     CDS             complement(59..337)
                     /locus_tag="VF516_28925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111803.1"
                     /translation="MHSTNLDTLPAIDLDSLTAVNGGDGWDDYKSRIAQDAHDTANRF
                     NQAVKYNAVNGNWNAGKFFDNLGGTFYNGAKTAIDAVPILGPAITNRL"
     gene            634..792
                     /locus_tag="VF516_28930"
     CDS             634..792
                     /locus_tag="VF516_28930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111804.1"
                     /translation="MKNTLKKLRLNKQQVRVLVADDLAQAIGGWIRPPLTWSCPQPSA
                     SECCPKTA"
     gene            979..1341
                     /locus_tag="VF516_28935"
     CDS             979..1341
                     /locus_tag="VF516_28935"
                     /inference="COORDINATES: protein motif:HMM:NF022443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
                     /protein_id="HEX8111805.1"
                     /translation="MAVTIAAGLGSRAVASALPWWLAKNAGDALYATMVFFGIGFIAP
                     RIRTFTAAATALVFCAAIEGSQAYHAPWLDAVRDTLPGHLVLGQGFHAFDLACYAIGV
                     ALGVALEIGALRRRDRAR"
BASE COUNT          230 a          542 c          618 g          278 t
ORIGIN      
        1 cgctccggcg ctatcctgcc acgaatcagg gtcgggcaag gtgcgcgcgg cccggccgct
       61 acagccggtt cgtgatcgcc gggccgagga tcggaaccgc gtcgatcgcc gtcttcgcgc
      121 cgttgtagaa cgtgccgccg aggttgtcga agaacttgcc cgcgttccag ttgccgttga
      181 ccgcgttgta cttcaccgcc tggttgaagc ggttggccgt gtcgtgcgcg tcctgggcga
      241 tccggctctt gtagtcatcc cagccatcgc cgccgttgac ggcggtgagc gaatcgaggt
      301 cgatggccgg cagcgtgtcg aggttcgtgc tgtgcatgag tgtgctcctg ttgcgtgatt
      361 ggctgcgcga cgcaaagcac gtcgaatgcc actgtgcggg gcccgcgatc tcgagggctt
      421 gccggatgca ccggcgcatg gtcatcgccc gcggtgagca acgcgcgcct accgcagaac
      481 tcgcgaggcg gtcgaggatc gggcgccgga tcggcgcgcc gattctgcac gccgcgagct
      541 cacccgcggc gcgctggatc caggtggccg acaggacggg aagatcatgc ggtacgcccg
      601 ttgcactccg gatcggtcca gcgaggaatg aacatgaaga acaccctcaa gaagctccgt
      661 ctgaacaagc agcaggttcg cgttctcgtg gccgatgacc tggcgcaggc gatcggcggc
      721 tggatccggc ccccgctcac gtggagctgc ccgcaaccgt cggcctccga gtgctgcccg
      781 aagacggcct agcctcgcga gtgcgctgac gagccccgcg gtggccagcc gtccgccgag
      841 tgtcacgcca ggtaccgtac accggggcgg cgagccgcat gatcgcagtg gtgtcgcgca
      901 ggcgggggtt ctagcatcgt cgcgatggtg cacctgggcg tccctgcgat ccgctcgcgg
      961 tggttgtatg cggtggccgt ggctgtcacg atcgccgcgg ggctcgggtc gcgcgcagtg
     1021 gcctcggcgc tgccctggtg gctggccaag aacgccgggg atgcgctcta cgcgacgatg
     1081 gtgttcttcg gcatcgggtt catcgcgccg aggatccgga cgttcaccgc ggcggcgacc
     1141 gcgctggtgt tctgcgcggc gatcgagggt tcgcaggcgt atcacgcgcc ctggctggat
     1201 gccgtgcggg acacgctgcc cggccacctt gtgctcgggc agggctttca cgcgttcgat
     1261 ctcgcgtgct acgcgatcgg ggtcgcgctc ggtgttgcgc tcgagatcgg cgccctgcgc
     1321 aggcgcgatc gcgcgcgatg acgaagcttc gcgaggatga gctgcgcgtc atcgcgaaca
     1381 acgtcgctcc gtacctgagc tccgattcac gctcaccagc gcgcccgtga tgggcacgaa
     1441 gaaagcgcgc ccccggcgac gcggtcgcgg cggccggcga ctatagtcgg cccgtgaaga
     1501 cgcgcgcagc tgtcgcactc gtgatcgcga tgaccgggag cgcggcggcg gagggcgagc
     1561 ggacgccgat gttcggcggc gcgctcacct acggcgatgc gccggacgga aggaccgcgc
     1621 tggtgggcgg cgagctcgag ctcgcgtggt ggtggggccg gctcgggc
//
LOCUS       DASVOR010001538         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_308196,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001538 DASVOR010000000
VERSION     DASVOR010001538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308196"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VF516_28940"
     CDS             complement(<1..351)
                     /locus_tag="VF516_28940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111806.1"
                     /translation="MEPIDLAAVADDFDREVAATPEIDRFCSSTAWILAAAAALMPPR
                     APASYRGADGYFAAMRGVHPAGFPYIEPVELAWGLASPLVGRDPMPLAEAVVATLAAR
                     RDWQLAIITGLTAWG"
     gene            477..1505
                     /locus_tag="VF516_28945"
     CDS             477..1505
                     /locus_tag="VF516_28945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111807.1"
                     /translation="MRALAVVLAGSLIAGCAVNSYKIPPAELARLARVPPEARGEHVR
                     VVQEVVASDVPAAPPVTRETQVVIAPQFEVAVGVHGPRAGFGGGFGRVLQGGKIGGLG
                     NDSKSAAVAFLFAAATAMVTAAVIEGSRFDGWAQLHPMHPVHLIGHDGSYTVVPLAWL
                     DPNAVAWADTAVVRPTEGPWLELERAPLSRQGLAYGLYGGTGSLRSAGGDLAMGPAWT
                     VQLGYFTTQQLGVFASVFFGWRTNRLDATLFESRYTAEVQYLPLQLGILHGGLFGGAG
                     VAYRFEDAIPLSDGRIAAGNEATLALTGGAMFQLDINTRVALTARLGLAQAHGEQMHD
                     AIFGVSVY"
     gene            complement(1534..1788)
                     /locus_tag="VF516_28950"
     CDS             complement(1534..1788)
                     /locus_tag="VF516_28950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1244 domain-containing protein"
                     /protein_id="HEX8111808.1"
                     /translation="MTAIPEALRTEIEAAAFRRLVQHFRDRTDVQNLDVMNLAGFCRN
                     CLSKWYRAAAAERGVEISDPEAREVIYGMPYDEWKAKYQK"
     gene            1828..>2058
                     /locus_tag="VF516_28955"
     CDS             1828..>2058
                     /locus_tag="VF516_28955"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEX8111809.1"
                     /translation="MAVTEIVSGETTFHVVGTAHVSAHSVDEVRQIIARVRPDAVCVE
                     LDQRRHDALTRDSAFRDLDVRGAVREGRALYVL"
BASE COUNT          262 a          750 c          752 g          294 t
ORIGIN      
        1 gccccaagcg gtcagcccgg tgatgatcgc cagctgccag tcgcggcggg ccgccagcgt
       61 cgcgaccacg gcctcggcga gcggcatggg gtcccggccg accagcggcg aggcgagccc
      121 ccacgcgagc tcgaccggct cgatgtaggg aaacccggcg ggatgcacgc cgcgcatcgc
      181 cgcgaagtag ccgtcggccc cgcggtacga cgccggtgcc cgcggcggca tcagcgcggc
      241 cgccgcggcg aggatccacg cggtcgacga gcagaaccgg tcgatctccg gggtcgcggc
      301 cacttctcga tcgaagtcgt cggcgactgc cgcgaggtcg atcggctcca cgcgcccatc
      361 gtagcgtatg gcgcccgggt cgctgccccg tgccggcgat gtgatctatc cccgccccgg
      421 ccccgccccc gccactccgc gcgctacggg catgaagaat ttgcaatact gcgcacatgc
      481 gtgcgctcgc ggtggtgctc gccggctcgc tgatcgcggg ctgcgcggtg aacagctaca
      541 agatcccgcc ggccgagctg gcgcggctgg cgcgcgtgcc gccggaggcc cgcggcgagc
      601 acgtgcgggt cgtccaggag gtcgtcgcca gcgatgtccc ggcggcgccg ccggtcaccc
      661 gcgagaccca ggtcgtgatc gcgccgcagt tcgaggtcgc ggtcggcgtg cacggccccc
      721 gcgccgggtt cggcggcggg ttcggcaggg tcctccaggg cggcaagatc ggtggcctgg
      781 gcaacgacag caagagcgcg gcggtcgcgt tcctgttcgc cgccgcgacc gcgatggtca
      841 ccgcggcggt gatcgagggc tcgcggttcg acggctgggc ccagctgcac ccgatgcatc
      901 cggtccacct gatcggccac gacggctcct acaccgtcgt cccgctggca tggctcgacc
      961 ccaacgcggt ggcgtgggcc gacaccgcgg tggtgcggcc gaccgagggc ccgtggctgg
     1021 agctcgagcg cgccccgctg tcgcgccagg ggctggcgta cggcctgtac ggcggcaccg
     1081 gctcgctgcg gtcggccggc ggcgacctcg cgatgggccc ggcgtggacc gtccagctcg
     1141 gctacttcac gacccagcag ctcggggtgt tcgcctcggt gttcttcggc tggcgcacca
     1201 accggctcga cgcgacgctg ttcgagagcc ggtacaccgc ggaggtccag tacctgccgc
     1261 tccagctcgg catcctgcac ggcgggctgt tcggcggcgc cggcgtggcc taccgcttcg
     1321 aggacgcgat cccgctgtcc gatggccgga tcgccgccgg caacgaggcg accctggcgc
     1381 tgaccggcgg cgcgatgttc cagctcgaca tcaacacccg ggtcgcgctg accgcgcgcc
     1441 tgggcctcgc gcaggcccat ggcgagcaga tgcacgacgc gatcttcggg gtcagcgtgt
     1501 actgaccggc gcggcagccc cccggcgggc cgctcacttc tggtacttgg ccttccactc
     1561 gtcgtacggc atcccgtaga tcacctcgcg ggcctccgga tcgctgatct cgaccccgcg
     1621 ctcggccgcc gccgcgcggt accacttcga caggcagttg cggcagaacc cggccaggtt
     1681 catgacatcg aggttctgaa cgtcggtgcg gtcgcgaaag tgctgcacca ggcggcggaa
     1741 ggccgcggct tcgatctcgg tgcgaagggc ttcagggatc gccgtcatgc ggtgtgtgta
     1801 gcgcagcgcc cgtggtatgt cagcgccgtg gcggtgaccg agatcgtgtc cggcgagacc
     1861 acgttccatg tggtcggcac cgcgcatgtc tcggcgcaca gcgtcgacga ggtccgccag
     1921 atcatcgcgc gggtccggcc cgacgcggtg tgcgtcgagc tcgaccagcg gcgccacgac
     1981 gcgctgaccc gcgacagcgc gttccgcgat ctcgacgtgc gcggcgcggt gcgcgagggc
     2041 cgcgcgctgt acgtgctc
//
LOCUS       DASVOR010001539         1621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_483405,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001539 DASVOR010000000
VERSION     DASVOR010001539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483405"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VF516_28960"
     CDS             complement(<1..462)
                     /locus_tag="VF516_28960"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8111810.1"
                     /translation="MVHFSGHGGRGEGRRSAGARRDVSEPTSGDHGAMEANDAERHGL
                     FFQGPDGQPQFVSAAAIQEAFAAAGSSVKLVVLNACYSEFLADALLPHVGCVVGVAGT
                     IGDASARQFSVGFYGGLGQRRSVEQAFAQGRAAVRLDGLQDHAQPQLRVRAD"
     gene            713..928
                     /locus_tag="VF516_28965"
     CDS             713..928
                     /locus_tag="VF516_28965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111811.1"
                     /translation="MRVLPIVAMLGCGSPMHAVDAPAADTAIPDAPVDAAAPVKANHQ
                     RGRHGLGSGFTTSSHGAASSTAGATRR"
     gene            complement(967..>1621)
                     /locus_tag="VF516_28970"
     CDS             complement(967..>1621)
                     /locus_tag="VF516_28970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111812.1"
                     /translation="GAASTREMPVVGSKPRTRRSRLVGWLLATVALGAAGVFAWPLLE
                     RGLAREDEPVRDAAVMVVTSAIDATTPDATTPDAAVLVDPPPSPADAIVAKTKQLAAD
                     GKVSAALDQLRHARQIYPESAQLPLLAGKLAMSQLYFSEGIMSFRAAIKLDPAVRTDP
                     ELVHAAIRAFTITPSVDAELARFVTELGAAAWPELDEIAKSHPNPAIRARAARLRGP"
BASE COUNT          239 a          574 c          577 g          231 t
ORIGIN      
        1 gtcggcgcgg acacgcaact gcggctgagc gtggtcctgg agaccgtcga gacgaacggc
       61 cgcccgcccc tgagcaaacg cttgctcgac cgatctgcgc tgtccgaggc caccgtagaa
      121 tccgacggag aactgtcggg ctgacgcgtc tccgatggtt ccggccacgc ccacgacgca
      181 gccgacgtgt ggcaacagcg cgtccgccag gaactcgcta tagcaggcgt tgagaacgac
      241 cagcttcacc gaggagccag ccgcggcgaa cgcctcctgg atcgcagccg cggacacgaa
      301 ctgtggctgg ccatcgggtc cctggaagaa caggccgtgc cgctcggcgt cgttcgcctc
      361 catggcgccg tggtcaccgg aggtgggctc gctgacgtct ctgcgcgcgc cggcggatcg
      421 tcggccctca ccgcgaccgc catggccgct gaagtggacc accgtcggcg tcagggtccc
      481 cagcccgtcg agcaggtcga gcggtcgcgc gggctggcaa ggactcccgc gcgcgccgcg
      541 gcgagagctc ggcgatgagc ggagcagcgc cggcagctcg cccgtccggc cgggccgctg
      601 ccgccgcgcg tggtgtcttg tcaacgttcg acgagatggc cgaggaagcg ctcgccgcga
      661 agtacagctg atcgccgcct cttgccggct cgagttggtg catcatgatc gaatgcgcgt
      721 cctgccgatc gtcgcgatgc tcggctgcgg ctcaccgatg cacgccgtcg acgccccggc
      781 cgccgacacc gccatccccg acgcgcccgt cgacgccgcc gcgcccgtca aagcgaacca
      841 tcagcgcgga cgacatggcc tcggcagcgg tttcacgacc tcctcgcacg gcgctgcgtc
      901 gtcgacggct ggagcgacga ggcggtgaca tgtttccgac cgcgctatcc ttcggtgcac
      961 gcgctctcac ggaccgcgca aacgtgcggc acgcgcgcgg atcgcgggat tcgggtgcga
     1021 ctttgcgatc tcgtcgagct ccggccacgc tgctgcgccg agctcggtca cgaagcgtgc
     1081 cagctccgcg tcgaccgagg gcgtgatcgt gaacgcacgg atggcggcgt gcacgagctc
     1141 gggatcggtg cgcaccgcgg ggtcgagctt gatcgccgca cgaaacgaca tgatgccctc
     1201 ggagaagtac agctggctca tcgcgagctt accggcgagc aggggaagct gggcgctctc
     1261 gggatagatc tggcgcgcgt ggcgcagctg gtcgagtgcg gccgagacct tgccatcggc
     1321 ggcgagctgc ttcgtcttcg caacgatcgc gtcggcgggg ctcggcggcg ggtccacgag
     1381 caccgcagcg tcgggcgtgg tggcgtcggg cgtggtggcg tcgattgccg acgtgaccac
     1441 catgacggcg gcgtcgcgga cgggctcgtc ctcgcgggcg agcccgcgct cgagcagcgg
     1501 ccacgcgaac acccccgcgg caccgagtgc gacggtagcg agcaaccacc ctaccaggcg
     1561 cgatcgccgc gtccgcggtt tgctgcccac caccggcatc tcgcgcgtcg atgcggcgcc
     1621 g
//
LOCUS       DASVOR010001540         4423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_541851,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001540 DASVOR010000000
VERSION     DASVOR010001540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541851"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VF516_28975"
     CDS             <1..188
                     /locus_tag="VF516_28975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111813.1"
                     /translation="YGMYLTDGGNSYISATTDAAGVMGISGSALGAIRPHDFEMVGGG
                     QRIGRHDYDCTRDPIRN"
     gene            complement(482..637)
                     /locus_tag="VF516_28980"
     CDS             complement(482..637)
                     /locus_tag="VF516_28980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111814.1"
                     /translation="MLLHSENTEERDGQTVEPWRSEWSEVHRRLREYARIGPRLAAPR
                     ACSEGAG"
     gene            1158..2399
                     /locus_tag="VF516_28985"
     CDS             1158..2399
                     /locus_tag="VF516_28985"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8111815.1"
                     /translation="MAERDTVNTGKGSSASEPDLRPRGATVVQAQAVGKRTLAESVVQ
                     PRSAAPGADGAGAAPAGAGDTTAVHDAAAHGISGSSAALPYAGQIQRLFGRHDISNIE
                     AHTDAPAAAGARAMGAEAYATGNHVAFASQPDLHTAAHEAAHVVQQRAGVQLKGGVGE
                     VGDPYERHADAVADRVVQGKSAEDLLSGFGGGAGVDAPVQKKDGKITHQGNVGSGQVT
                     ARENDLDPSDKSNDNYSLDYTGKDADKAHWLQFVNFSMIAEVPGTGPVYNTGSIGTTS
                     GHKPFSTDKVTNWSVDSADTSNPYYEAAGSNVRTPGKGTKIFDEPGGPSIIGIADQFV
                     STKAAKATKVTFIARFDTYLVIDGTATYHVVWSATTEYDPAKKTTGAIAYATGTAGDA
                     KGLPKDFKTVLDTAYSGNKIK"
     gene            2492..2884
                     /locus_tag="VF516_28990"
     CDS             2492..2884
                     /locus_tag="VF516_28990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111816.1"
                     /translation="MSHSDAEGLWQQAREVPAPGTEGHSLADTYTFRYQGGLVTLHLM
                     TITKVSWRSPDDFYTLKSRWQGDTLQYLTPVGQWIDLATFEGGRFVASGDGKRREYDR
                     VTPDQIAGFSAGILKPDRVAFDYSRSMK"
     gene            3037..3861
                     /locus_tag="VF516_28995"
     CDS             3037..3861
                     /locus_tag="VF516_28995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111817.1"
                     /translation="MRIRPVLAITCCLLGAGSLLLEAHNTTHGDGPLMTGMAVIVVVA
                     AALLALRPLWAALAGRGLLWTVLALTTLIDSISHPIWTATAAALAMCAALLALGRHAL
                     EGPTAAFRPNHHRGLLTLAMVLGFADVATLSTWSIFAFASGDGRALLFGLVFTAIAAA
                     IAASLVGLYRLYAWGFLLNLAVNLAVTVLMMFDVFRFDVFRLVFIVPAAAQILLALPV
                     LVAILLRRPLTVPRPLARLGALVPALTVLVMAGLNVQSWFGEPALRQLVRWGMGHL"
     gene            complement(3862..>4423)
                     /locus_tag="VF516_29000"
     CDS             complement(3862..>4423)
                     /locus_tag="VF516_29000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111818.1"
                     /translation="SKRRPNQGVLSWVEFTPALDLFVSTVSFGEIELGLEMERSRNPT
                     FAAHLGDWLELTLRAYRDRILPLAQAAIAPQRARVARTRDDFAHLDPHQRAELDAAVA
                     DAARAIQDRILTALASGELRPATFKPMTGVAIARDVLDIVDRGNTRFVGSLTPDQQTE
                     LASHRFDFADYLVFSAHWEDALHALD"
BASE COUNT          684 a         1552 c         1533 g          654 t
ORIGIN      
        1 actacggcat gtacctcacc gacggcggca acagctacat ctccgcgacg accgacgccg
       61 ccggcgtgat gggcatcagc ggctccgccc tgggcgcgat ccggccgcac gacttcgaga
      121 tggtcggcgg cggccagcgc atcggccgcc acgactacga ctgcacgcgc gacccgatca
      181 ggaactgacc aggcggcaac tggcagctcg ccgcactcat ctgacgtttc gccgcagccc
      241 atgcgaaggc cgtgaccggt ggccgagcgg tggccgggcg gtggccgccc ggaccgccgt
      301 gccccggcaa gacaccgaac aggatggcca gccccgccgc gatgtgcccc gcgccggtca
      361 agtacaccca gtcgatccgc agcgggaacc acgtcggcac gtacgcggtc gccgcgggcg
      421 ttcaggaagt gcgcgaagcc gacgagcggg agacccgcgg cgtagacgca ccgcgcgagc
      481 ctcaccccgc gccctccgag cacgcgcgcg gcgcggccag ccggggaccg atccgcgcgt
      541 actcgcgcag ccggcgatgg acctcgctcc actccgaccg ccacggctcg acggtctgtc
      601 cgtctcgctc ctcggtgttc tcgctgtgca gcagcatcga cgttcccttt cgcagacacc
      661 tggctcgtgt gtgtgccgtc tgtatcacgg tggttttcga cgcatcctgc tgcgatctcc
      721 cgcacggaaa tgaccctggc cgcgattttg acccgccacc acctgttcat ccggaatcga
      781 ccttcggtga tcgagacacg atcgcgcgac gccaacgcga gggcgccgtg ggtgatcgcg
      841 agcaggacgg ggtcggggca gcgaccgtga ttggactggc cgggtcttgt tccgcacata
      901 cctgacgccg tccgcgcagg ggtcggtggt gattcggcgg gcgcggccac gacgcccatc
      961 cgcgcagccc acggcgagcc gagcgtgcac gcgttcttcc cgagctatgc cctacgagtt
     1021 cctggaccgc ggtccgcggt ggggtgcggg gcgtcgcacc ccgccagatc tggatgcaat
     1081 ccgcggcgtt accggcacgt ggcgcatcga gggcaacttc cgaggacccg gccgtatagt
     1141 ggtgggaggt acgtaccatg gcggagcgcg acacggtgaa cacgggcaag ggatcgagcg
     1201 cgtcggaacc cgacctgcgg ccccgcggag ctaccgtcgt acaggcgcag gccgtcggca
     1261 agcgcacgct cgcggagtct gtcgtccagc ctcggtccgc ggcgcccggc gccgacggtg
     1321 cgggtgcggc cccggccggc gcgggcgaca ccaccgcggt gcacgacgcg gccgcacatg
     1381 gcatctccgg cagctcggct gcattgccct acgccgggca gatccagcgc ctgttcgggc
     1441 gccacgacat cagcaacatc gaggcccaca ccgatgcacc cgcggcggcc ggcgcgcggg
     1501 cgatgggcgc cgaggcatac gccaccggca accacgtggc gttcgcgtcg cagccggatc
     1561 tgcacaccgc ggcgcacgag gcggcgcatg tcgtgcagca gcgcgccggc gtccagctca
     1621 agggcggggt cggcgaggtc ggcgatccgt acgagcgcca cgccgacgcg gtcgccgatc
     1681 gcgtggtgca gggcaagtcg gccgaggacc tgctgtccgg gtttggcggc ggggccggcg
     1741 tcgacgcgcc ggtccagaag aaggacggca agatcaccca ccagggcaac gtcggctcgg
     1801 gacaggtcac ggcgcgcgag aacgacctcg atcccagcga caagtccaac gacaactact
     1861 cgctcgacta caccggcaag gacgccgaca aggcgcactg gctgcagttc gtcaacttct
     1921 cgatgatcgc cgaggtcccg ggcaccggac cggtctacaa caccgggtcg atcggcacga
     1981 cgagcggcca caagccgttc tcgaccgaca aggtcacgaa ctggtcggtg gattcggccg
     2041 atacctccaa tccgtactac gaggcggcgg gttcgaacgt ccggactccg ggcaagggga
     2101 ccaagatctt cgacgagccc ggtggcccat cgatcatcgg gatcgccgat caattcgtct
     2161 cgaccaaggc ggccaaggcc accaaggtca cgttcatcgc ccgcttcgac acctacctgg
     2221 tcatcgatgg cacggcgacg tatcacgtcg tctggagcgc gacgaccgag tacgatccgg
     2281 ccaagaagac caccggcgcg atcgcgtacg cgacgggcac ggcgggcgat gccaagggcc
     2341 tgcccaagga cttcaagacc gtcctcgaca ccgcctactc cggcaacaag atcaaatgag
     2401 ccgcaggagc cacacgacgg catggcccag gatcgtctgt ctgatcctgc tggccgcgag
     2461 cgcctgcggt cacaccagca aaggaaccga catgagtcac agcgatgcgg aagggctgtg
     2521 gcagcaagcc cgcgaggtcc cggctcccgg caccgagggc cacagcctgg ccgacaccta
     2581 caccttcagg taccagggcg gcctggtcac gctgcacctg atgacgatca ccaaggtgag
     2641 ctggcggtcg cccgacgact tctacacgct gaagtcgcgc tggcagggtg acacgctgca
     2701 gtacctgacg ccggtgggtc agtggatcga tctggcgacg ttcgagggcg gccggttcgt
     2761 cgcgtcgggt gacggcaagc gccgtgagta cgaccgggtc acgcccgatc agatcgccgg
     2821 gttcagcgcc ggcatcctca agcccgaccg tgtggccttc gactactccc gatcgatgaa
     2881 gtgatcgatc cgggagctcg ccgtggcgga gcagggcatc atgccttgca gatccgcagg
     2941 atacgcgatc ggtggacggc ccggggatgg ctctcacgac gccgaacccc ccgggaggaa
     3001 atcgaatgcg cgcccggtgc gttgcatcct cccgagatgc gcatccgccc cgtcctcgcg
     3061 atcacctgct gtctccttgg cgccggctcg ttgctgctgg aggcccacaa caccacgcat
     3121 ggcgacggcc cgctcatgac gggcatggcc gtgatcgtgg tggtcgctgc cgcgctgctc
     3181 gccctgcgcc cgctgtgggc cgcgctcgcc ggccggggcc tgctgtggac cgtcctggcg
     3241 ctgaccacgc tgatcgacag catctcgcac ccgatctgga cggccaccgc ggcggcgctc
     3301 gcgatgtgcg cggcgctgct cgcgctcggg cgccacgccc tcgagggccc gacggccgcg
     3361 ttccggccga accaccaccg cggtctgctc accctcgcga tggtgctcgg gttcgccgac
     3421 gtcgcgacgc tgagcacgtg gtcgatcttt gcgttcgcgt ccggcgatgg gcgcgcgctg
     3481 ctgttcgggc tcgtgttcac cgcgatcgcc gctgcgatcg ccgcatcgct cgtcgggctg
     3541 taccggctgt acgcctgggg cttcctgctc aacctcgcgg tcaacctcgc ggtcacggtc
     3601 ctgatgatgt tcgacgtgtt ccgcttcgac gtcttccggc tggtgttcat cgtgccggcg
     3661 gccgcgcaga tcctgctcgc gctgccggtg ctggtggcga tcctcctccg ccgcccgctc
     3721 accgtgccgc ggccgctcgc ccggctgggg gcgctggtgc cggcgctcac ggtgctggtc
     3781 atggcgggcc tgaacgtgca gagctggttc ggcgagccgg cgctccgcca gctggtccgc
     3841 tggggcatgg ggcacctgta gtcagtcgag cgcgtgcagc gcgtcctccc agtgcgcgct
     3901 gaacaccagg tagtcggcga agtcgaagcg gtgcgatgcg agctcggtct gctgatccgg
     3961 cgtcagcgag cccacgaacc gcgtgttgcc ccggtcgacg atgtcgagca cgtcgcgcgc
     4021 gatcgcgacg ccggtcatcg gcttgaacgt cgcggggcgc agctcgccgc tcgcgagcgc
     4081 ggtcaggatg cgatcctgga tcgcccgggc ggcgtccgcg accgccgcgt cgagctcggc
     4141 gcgctggtgc ggatcgaggt gggcgaagtc gtcgcgcgtg cgggcgaccc gggcgcgctg
     4201 cggtgcgatc gccgcctggg cgagcggcag gatgcgatcg cggtaggcgc gtagcgtcag
     4261 ctcaagccag tcgccgagat gggcggcgaa cgtcggattc cgcgagcgct ccatttcgag
     4321 tccgagctcg atttcgccaa agctgacggt gctgacgaag aggtcgagtg cgggcgtgaa
     4381 ctcgacccag gaaaggacac cttgattggg ccgccgcttg ctg
//
LOCUS       DASVOR010001541         1699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_161588,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001541 DASVOR010000000
VERSION     DASVOR010001541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1699
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161588"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VF516_29005"
     CDS             complement(<1..1096)
                     /locus_tag="VF516_29005"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8111819.1"
                     /translation="MMQRRTFLHGAAVVGAAALLPRRAHAAATLKPIYDQIEKQRAET
                     IARIQEWIKQPTIAAESRGITEGRDLTMRLLRDAGFGKVESVSTDGHPGIFATLDAGA
                     KRTIGLYFMYDVKQVDPKEWSSPPWDAAIVDVPKLGKAIMGRGAVNQKGPQATFLAAL
                     HAIRGANRKLPVNLVFVAEGEEEIGSPHFAQVVSRPDIRAQLKKCDGIFMPHASQSTD
                     GTVTINLGAKGVVECEIVSSGDTWGRGPKKAIHSSNRARVDSPAWHLVQALNTLVNAE
                     GDPAIDGLFERVKPLSERDRKLLDLAAQQMNEDEQKAALGVQRWAHDASFRQSLEEFV
                     ARPTVNIEGIYGGYTGPGGMTILPHRAAAKL"
     gene            1135..1464
                     /locus_tag="VF516_29010"
     CDS             1135..1464
                     /locus_tag="VF516_29010"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX8111820.1"
                     /translation="MSLYALNLFDLADNDDYLAYSRRSVAAVGKHGGKVVALGRLDAE
                     ATPEAGDTAPRQVMVLVEWPDRAAFEAFQDDPGHHDLHPLRRNGTRNYLWWAYERLED
                     LRPLLKR"
     gene            1479..>1699
                     /locus_tag="VF516_29015"
     CDS             1479..>1699
                     /locus_tag="VF516_29015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111821.1"
                     /translation="MRSAPVLAVILSLIIASGGACAGPAPEPESAAEAGPRYVSIGRD
                     ALDAALAVAERRGAALQVLDLDADVAVIAV"
BASE COUNT          214 a          608 c          594 g          283 t
ORIGIN      
        1 cgagcttggc ggcggcgcgg tggggcagga tggtcatgcc gccggggccg gtgtagccgc
       61 cgtagatgcc ctcgatgttg accgtcggcc gggccacgaa ctcctcgagc gactggcgga
      121 agctcgcgtc gtgcgcccag cgctggacgc cgagcgccgc cttctgctcg tcctcgttca
      181 tctgctgggc ggccaggtcg agcagcttgc gatcgcgctc gctcagcggc ttgacccgct
      241 cgaacagccc gtcgatcgcc ggatcgccct cggcgttgac cagcgtgttc agcgcctgga
      301 ccaggtgcca cgccgggctg tcgacccggg cgcggttgct ggagtggatc gccttcttgg
      361 ggccgcggcc ccaggtgtcg ccgctcgaca cgatctcgca ctcgacgacg cccttggcgc
      421 cgaggttgat cgtcacggtg ccgtcggtgc tctggctggc gtgcggcatg aagatgccgt
      481 cgcacttctt gagctgggcc cggatgtccg gccgcgacac gacctgcgcg aagtgcggcg
      541 agccgatctc ctcctcgccc tcggcgacga acaccagatt gacgggcagc ttgcggttgg
      601 cgccgcggat cgcgtggagc gcggcgagga acgtcgcctg gggccccttc tggttgaccg
      661 cgccgcggcc catgatcgcc ttgccgagct tggggacatc gacgatcgcg gcgtcccacg
      721 gcggcgagct ccactccttg gggtcgacct gcttgacgtc gtacatgaag tacagcccga
      781 tcgtgcgctt cgcgccggcg tcgagcgtcg cgaagatccc ggggtgcccg tcggtcgaca
      841 cgctctcgac cttgccgaac ccggcgtcgc gcaagagccg catcgtcagg tcgcggccct
      901 cggtgatgcc gcggctctcg gcggcgatcg tcggctgctt gatccactcc tggatccgcg
      961 caatggtctc ggcgcgctgc ttctcgatct gatcgtagat cggcttgagg gtggcggccg
     1021 cgtgcgcccg ccgcggcagc agcgcggcag ccccgacgac cgccgcgcca tgcaggaagg
     1081 ttcggcgttg catcatggcg cgcgactata cacctcgcct ggtacgttcc cgccatgtcg
     1141 ctctacgcat tgaacctgtt cgatctcgcc gacaacgacg actatctggc ctactcccgg
     1201 cgcagcgtcg ccgcggtcgg caagcacggc ggcaaggtgg tcgcgctcgg ccggctcgac
     1261 gccgaggcga ctcccgaggc cggtgatacc gcaccgcgcc aggtcatggt gctcgtcgag
     1321 tggccggatc gcgcggcgtt cgaggcgttc caggatgacc cggggcacca tgacctgcac
     1381 ccgctgcgcc gcaacgggac ccgcaactac ctgtggtggg cgtacgagcg gctggaagac
     1441 cttcgcccgc tgctcaagcg ctgagctaca ctgcagccat gcgcagcgcg cccgtcctcg
     1501 ccgtgatcct ctcgttgatc atcgcctctg gcggcgcctg cgccggccct gccccagagc
     1561 cggagtccgc tgccgaggcc ggcccgcgct acgtatcgat cggccgggac gcgctcgacg
     1621 cggcgctcgc ggtcgccgag cggcgcggcg cggcgctgca ggtgctcgac ctcgacgccg
     1681 acgtcgcggt gatcgcggt
//
LOCUS       DASVOR010001542         4191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_16664,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001542 DASVOR010000000
VERSION     DASVOR010001542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16664"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="VF516_29020"
     CDS             <1..1686
                     /locus_tag="VF516_29020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111822.1"
                     /translation="PTRAVIAAYALYALSQTEHNKPGDAEIAALIAAPTTDPCARVIA
                     TLAFEAASHDVPRARSLMADAGAAADQCGDERLKADLLIRDIRFRIERPVVGAKGAAA
                     IQHAQIAVARVDQPDVAAKLAEATVEVALQRRNWSEVVRIADTAVSGYRARGLPVRML
                     NAVIERNFMHLVRSEPGDLDAIAADARAFRPLAVAHHRAELARRLDVDDAIARYRRGD
                     PAATDDFVRLWQAQSPGERSSRARRVTGEVVDAAGRAVAGARIAASNILIANATRIGL
                     PQLRKDQGSRDNELRIATSDPAGRFVIEDCAPDDSIAAELGDHRSEPVPVPDHVRLVL
                     EPTRQITGRVNLAGTPATRVYVRVEIGDELAGQIELVAPVAPDGSFTIGGAPQGAVRI
                     GVVLRGGEDGDRFDPRPLPASAGPTTGVSLGLAQPSRVLDVVVRSAISAPLEGAQVFL
                     IRGKPAIRSLGDLLRAAHTGIHSRLARPVAGEDVPHAVVGKIRPGDLLAHLEHAEPGE
                     LTVCALSFSGDLLDPDFWPRLRAHESELAVRCEPIGPTTAIVEVAAPPQPSFE"
     gene            complement(1913..2350)
                     /locus_tag="VF516_29025"
     CDS             complement(1913..2350)
                     /locus_tag="VF516_29025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111823.1"
                     /translation="MMLVCALGALGAVGCVGTSDAADEAAVAQNTGGGGALFDCKNVA
                     SISVVQCVGSILSANVINVNVKDVNVLSGDQLKVLDDDLNNLNIGDINILNVNDVLNK
                     VQVTALNDFLTKFNINVDKNNLPIANDIDVCALVGIAQICKNG"
     gene            complement(2856..>4191)
                     /locus_tag="VF516_29030"
     CDS             complement(2856..>4191)
                     /locus_tag="VF516_29030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111824.1"
                     /translation="RGRESPLSIDPWAAPPPPKPATTESRQRRAEAALGRVAKIEPEV
                     ARLRHLAFERAVPTRYQKSDEFRAFVRREIAKELPAARSRDLTAALAHLGFLARPTDL
                     AGVEEQAMTTQAGAYYDPAAKAFFVVMVPSSDLMLDTMSAHELTHALQDQHFDLERYL
                     PADSKLDDDAATARRFVAEGDATLVMLLYTMRTMLGDQLTPPVLAVLHDQIEKLASQD
                     LAALEAQVRQQSALFGGIDAEIQKSINAMDDIPPAVLVPLLDSYMKGALVALTAYERG
                     GWAAVDALYRDPPSSTEQVLHPATKLYPVRDRPRRVTLARGTDPELAGNVLGELQWKI
                     YFELWKVPHAAEAAAGWGGDRYSVTRRRDGRLIGRIATVWDTPDDARQFADAYAASLS
                     ARFPGAGSLAAGIARPDGGRVFVRVAGDRVFIVDGADDAGALDALVKSTKLD"
BASE COUNT          622 a         1473 c         1454 g          642 t
ORIGIN      
        1 ccgacgcgcg cggtgatcgc ggcctacgcg ctgtatgcgc tcagccagac cgagcacaac
       61 aagccgggcg acgccgagat cgccgcgctg atcgccgccc ctaccacgga tccgtgcgcc
      121 cgggtgatcg cgacgctggc gttcgaggcc gcatcgcacg acgtgccacg ggcgcgctcg
      181 ctgatggccg acgccggcgc cgccgccgat cagtgcggcg acgagcgtct caaggccgat
      241 ctgctgatcc gggacatccg gtttcgcatc gagcggccgg tggtcggcgc caagggggcg
      301 gcggcgatcc agcacgcgca gatcgccgtc gcgcgcgtcg accagcccga cgtcgcggcg
      361 aagctcgccg aggccacggt ggaggtggcg ctgcagcgcc ggaactggag cgaggtggtc
      421 cggatcgccg acaccgcggt cagcggctac cgcgcgcgcg ggctgccggt ccggatgctg
      481 aacgccgtga tcgagcgcaa cttcatgcac ctggtccgca gcgagcccgg cgacctcgac
      541 gcgatcgccg ccgacgcccg ggcgttccgg ccgctcgcgg tcgctcacca ccgggccgag
      601 ctcgcccgcc ggctcgatgt cgatgatgcg atcgcacggt accggcgcgg cgatcccgcg
      661 gcgaccgacg acttcgtgcg gctgtggcag gcgcagtcac cgggcgagcg gtcgagccgg
      721 gcgcgccggg tcaccggcga ggtcgtcgac gccgccgggc gcgcggtcgc cggggcccgg
      781 atcgcggcgt cgaacatcct gatcgccaac gccacgcgca tcggcctgcc ccagctgcgg
      841 aaggaccagg gctcccgcga caacgagctg cggatcgcga ccagcgaccc cgcggggcga
      901 ttcgtgatcg aggactgcgc accggacgac tcgatcgcgg ccgagctcgg cgatcaccgc
      961 tcggagccgg tcccggtccc cgatcacgtc cgcctcgtgc tggagccgac ccgccagatc
     1021 accggccggg tgaacctcgc aggcaccccg gcgacccggg tctacgtccg ggtcgagatc
     1081 ggcgatgagc tggcgggcca gatcgagctc gtcgcgccgg tcgcgccgga cggctcgttc
     1141 acgatcggcg gagcccccca gggcgcggtc aggatcggcg tcgtgctccg cggtggcgag
     1201 gacggtgacc gcttcgatcc ccggccgctg ccggcgtcgg ccgggccgac cacgggcgtc
     1261 tcgctcggcc tcgcgcagcc cagccgggtg ctcgacgtcg tggtgcgcag cgcgatctcg
     1321 gcgccgctcg agggcgccca ggtgttcctg atccgcggca agccggcgat ccggtcgctc
     1381 ggcgatctgc tccgcgccgc gcacaccggc atccactcgc ggctcgctcg tccggtcgcg
     1441 ggcgaggacg tcccgcacgc cgtggtcggc aagatccgcc ccggcgatct gctggcgcac
     1501 ctcgagcacg ccgagcccgg cgagctgacg gtgtgcgcgc tcagcttcag cggcgatctg
     1561 ctggatcccg acttctggcc gcggttgcgc gctcacgagt cggagctcgc cgtgcgctgc
     1621 gagcccatcg ggccgaccac cgcgatcgtc gaggtcgccg cgccgccgca gccgagcttc
     1681 gaatagcgag cgttaggaca acagcaagcc aggggccggg cacgcagcgc ccgggcgtcc
     1741 cgtgaccggc gcaagcttcc ggccacgagt gattccctga cgttgcgcgc tcgacccctg
     1801 gctcatgcgg caatcccgcc gtggatcgga atcacgtcat ccccgggggt gggggagtgg
     1861 gagggattcc atgtcccgat ccacggcagt cggtaatgag gatctcgtga tcctatccat
     1921 tcttgcagat ctgggcaatg ccgaccaggg cgcagacgtc gatgtcgttc gcgatcggca
     1981 aattgttctt gtccacgttg atgttgaact tggtgaggaa gtcgttcagc gcggtcacct
     2041 ggaccttgtt gaggacgtcg ttgacattca ggatgttgat gtccccgatg ttcaggttgt
     2101 tcaggtcgtc gtccagcacc ttgagctggt cgccgctcag aacattgaca tccttgacgt
     2161 tgacgttgat cacgttggcg ctgaggatcg agccgacgca ctgaacgacg ctgatgctag
     2221 cgacgttctt gcagtcgaac agggcaccgc cgccgccggt gttctgcgcc accgcagcct
     2281 cgtccgccgc gtcgctggta ccgacgcagc caaccgcacc aagagcaccg agagcacaaa
     2341 cgagcatcat cgagcgaaac atagctacct ccagagagag aatcacacag gggtaatacg
     2401 ggctgattcc cgcattgcga atatcgcaat tcgagtattc gcatatcgcg gctattggcc
     2461 aatagcgatc ggcacctgtt tcacgccgct ctaacccctg tggaaatcgt ctaatgcgat
     2521 tgatactcgc gccgcgttgc cacactgaac ggcgtgtacc tgatctgtgc gatcgtgtca
     2581 ctggccatca tcgaaacgtg cggattcgcc acaggcagtt gcgcgcgatc acatcccgcc
     2641 gccgatcgtg cgccatcgca tgccgcacgc tgtagcagcg tgcactccgg ccccggattt
     2701 cggccggatc gggggtgccg atgtgccgca gaacgctgca tcatgagatg gtcgtgatcc
     2761 gattttgaca atcgatttct ggaaaacact gattcagacg ctgcgagatc gcgccgtcgg
     2821 atcgcgattc aggcaaatgg cgctcgggct ggacctcaat cgagcttcgt cgacttgacc
     2881 agcgcatcga gcgcgccggc gtcgtccgcg ccgtcgacga tgaatacccg atcgccggcg
     2941 acgcgcacga acacccggcc accgtcgggc cgcgcgatcc cggcggcgag cgagccggcg
     3001 ccggggaacc gcgccgagag gctggcggcg taggcatcgg cgaactggcg ggcatcgtcc
     3061 ggcgtgtccc agaccgtcgc gatccgaccg atcagccggc cgtcgcgccg gcgcgtcacc
     3121 gagtagcgat cgccgcccca gccggccgcc gcctcggcgg cgtgcggcac cttccacagc
     3181 tcgaagtaga tcttccactg cagctcgccg agcacgttgc cggcgagctc cgggtcggtg
     3241 ccgcgcgcca gcgtgacccg gcgcggccga tcgcgcaccg ggtacagctt ggtcgcgggg
     3301 tggagcacct gctcggtcga gctcggcgga tcgcgataca gcgcgtcgac cgcggcccag
     3361 ccgccgcgct cgtacgcggt cagcgcgacc agcgcgccct tcatgtacga gtcgagcagc
     3421 ggcaccagca cggccggcgg gatgtcgtcc atcgcgttga tcgacttctg gatctcggcg
     3481 tcgatcccgc cgaacagcgc ggactgctgc cggacctggg cctccagcgc ggcgaggtcc
     3541 tgcgacgcca gcttctcgat ctggtcgtgc agcaccgcca gcaccggcgg cgtgagctga
     3601 tcgccgagca tggtgcgcat cgtgtacagc agcatgacca gcgtcgcgtc gccctcggcc
     3661 acgaaccgcc gggcggtcgc cgcgtcgtcg tcgagcttgc tgtccgcggg caggtagcgc
     3721 tcgaggtcga agtgctggtc ctgcagcgcg tgggtcagct cgtgcgccga catcgtgtcg
     3781 agcatcagat cgctgctcgg caccatcacg acgaagaacg ccttggccgc cgggtcgtag
     3841 tacgcgccgg cctgggtcgt catcgcctgc tcctcgacgc cggccaggtc ggtcggccgg
     3901 gcgaggaacc cgaggtgggc gagcgccgcg gtcaggtcgc gcgatcgcgc ggccggcagc
     3961 tccttggcga tctcgcggcg gacgaacgcg cggaactcgt ccgacttctg gtagcgggtc
     4021 ggcacggcgc gctcgaacgc caggtgccgc agccgggcga cctccggctc gatcttcgcg
     4081 acccggccga gcgcggcctc ggcgcggcgc tggcgcgatt cggttgtcgc gggcttcggt
     4141 ggcggcggcg cggcccacgg gtcgatcgag aggggacttt cccgtcccct c
//
LOCUS       DASVOR010001543         8802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_541891,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001543 DASVOR010000000
VERSION     DASVOR010001543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541891"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="VF516_29035"
     CDS             complement(<1..918)
                     /locus_tag="VF516_29035"
                     /inference="COORDINATES: protein motif:HMM:NF045340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111825.1"
                     /translation="MQQTTRSLVAVVVIAGLVTGASVPRVGLAQPATTPVANTTPGTP
                     GAAGTPAAGEDDKLYNCKQLKTGQVSVTFKPETELKDLITWVMGFTCKNFIFEPRIVS
                     TGKKVTLIAPNKMTPTEAYRLFLVALSTMGLTVVPKGNALRIVESAIAKSETVPIYKR
                     GVPSDEDQIVRYVLRPTYAQVEIMRQALDSMRSPAGNVQVAGTMLIITDYASQVRDMM
                     SLARSIDVPGGSDGIYTIPIHHADATQLAQKLNDILGISAAAAAAGAGGRRPPNVPPQ
                     PGMPPAPNTPAASNEVAGAVPSKILVDDRS"
     gene            complement(924..1847)
                     /gene="gspC"
                     /locus_tag="VF516_29040"
     CDS             complement(924..1847)
                     /gene="gspC"
                     /locus_tag="VF516_29040"
                     /inference="COORDINATES: protein motif:HMM:NF041515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HEX8111826.1"
                     /translation="MQDLIKRHFWVLGAVTVVVCAVFAAKATNHIVEAKFLGDPEHAP
                     KITPVVAQTQAPVAAVRSKDGGQFQSRNMFCADCTPAVSTQSSDPSQIAQTSLPLALL
                     ATNIGPRPEDSYATIINSENQRQGAFSVGDQIPGATGKLKAIHFKYIDFENNGRTERL
                     VLLGATPPVTPVAAAEAPPALPTGDGDEMQAAVDSGIKKLDDNNYEIDKSLVEKVLLN
                     PMAVAKGARVVPSMKNGKPDGFKLYAIRPSSAFAKLGLANGDTLQSINGFELTSADKA
                     LEVYTKLREATSLEVEVTRRGKPVTLKYSIK"
     gene            complement(1851..3374)
                     /locus_tag="VF516_29045"
     CDS             complement(1851..3374)
                     /locus_tag="VF516_29045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8111827.1"
                     /translation="MSSPEDPGEAARTADGGIVLVVDDEPAIVESLTKIFKREGLTVL
                     SATDGTAGLDVLRKHRVGVLLTDLMMPNTSGMDLLRAAKTIAPETEVVLMTAYGTVET
                     AVDAMKEGAYDFVTKPLKRAHVVRIVRNALEKQSLLVENRSLKAQLAEKRRRAIIGTS
                     LAWRRTMDIVMQAAPSEATVLLLGESGTGKELLARALHDNSARARSGRQGDGPGPFVA
                     VNCAAIPESILEAELFGYEKGAFTGAATARDGRFEAANGGTLFLDEIGEISRHVQVKL
                     LRVLQEGEIERLGGSGKSRRIDVRIVAATNVNLAEEVKAGRFREDLYYRLNVIPVSVP
                     PLRDRRDDISLLAQHFVQVYAEKNGKAIAGCSPAALERLTEYGWPGNVRELENAIERA
                     VVLTRAGSSVIDEDALPREIRDATPGAASASSLTFPIGMPLAEIEMRVILETLRHTRG
                     DKRLTAKLLGIATRTIYRRLETTDPEAARDAREGSELPETAETETPGATDGAVAEED"
     gene            3652..3891
                     /locus_tag="VF516_29050"
     CDS             3652..3891
                     /locus_tag="VF516_29050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111828.1"
                     /translation="MRLPFDRERMRERNLLDDIGEIELAAAQSPAERLAATLALSDLT
                     LAFFRGNPDAPVLDRLEELEEKARLWAAPLRAIRR"
     gene            3957..4631
                     /locus_tag="VF516_29055"
     CDS             3957..4631
                     /locus_tag="VF516_29055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5131 family protein"
                     /protein_id="HEX8111829.1"
                     /translation="MVGGLAVGAWGVPRATKDVDLYAELPTAVRPRVRRELMQRNFDV
                     PAMDEELQRFGVFRSLFRPTSVFVDIFDAENPLGEAILNRRREVQVQGRIRWTAAAED
                     LAVLKAFSDRARDLFCLGWNVIVGGESGAGARPLDKQWVRSLRDQCAAAKVPFFFKQW
                     GGFQKGKHGRHLDGRTYDEMPTISARSVPGSAERTQRIVSLGEFAAAWKNVQFGAPRL
                     GSRAVS"
     gene            complement(4642..4851)
                     /locus_tag="VF516_29060"
     CDS             complement(4642..4851)
                     /locus_tag="VF516_29060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111830.1"
                     /translation="MASRERASTEPRRSIPDRHKRSDLIEWRREDLGLAWSIDLPDGA
                     EATARWLDAMTNAVDDRTPGGLGWR"
     gene            complement(5066..5656)
                     /locus_tag="VF516_29065"
     CDS             complement(5066..5656)
                     /locus_tag="VF516_29065"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8111831.1"
                     /translation="MQATLRLPRRASYADYLAVEQTSECRHEFIDGVIVAMAGGSDEH
                     NRIAGRLAQLLGSRETGSCGYYPSDQRFWIASRARGRYADGSIICGPPEHPAHDAQAT
                     TNPTMVIEVLSPSSEGDDDGEKRSDFQSLASLEAYVLVAQDQRRVKVYRRSGGEWGVT
                     TYRDGDSFELPTLSTSIPVADIYDRILDSAGKSLLR"
     gene            complement(5723..8509)
                     /locus_tag="VF516_29070"
     CDS             complement(5723..8509)
                     /locus_tag="VF516_29070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111832.1"
                     /translation="MSGTDDLQGADAGPSRSSSAISDPGPAGAPSGPSGLPFGDLPFK
                     RRLVVPAAVLGVAELVFIAYPMAVAFDLAGFGGDTLLRTVLPVGGGAVVVWLAAITAW
                     LMPLWSAVAARRRGERVHKELAARAYRITLNGPIRVLLLRTGLWAASAALIGLFLHVF
                     RDWPIERVAEITALAAIHAYILSCIRAVWWAQTLGELSARLFAAGSPLKRFDDNHFRR
                     FLLVAMIVAGGVLAAQAAFAYYFVPITFPQYLQFETYVAVASAAGVTAWLVVARMATR
                     DLRRYLAASRNEIVSGAPPAAVIYRRAQALPYRLAVMTIAVWLVIAVIGGFIARHLGF
                     ELDDTIVLTTAMTVLAIAGAIYEQLWHRDVLRPLLAHLTQRYRVPVRSIAPSLSLRAK
                     LLLSFGGVVLLACGMALLWGFVQYKSLATEAAGQQSRLGLQWIHSELQRALGSDPVAP
                     APDRVRGALRRITTSAPDAPVMYYLDDDGDLLAVGGGGLGAPRPPWYVRYQLQRATGE
                     LLAIHAAELTGRAQILEIGWRGARYRLGAVAVFYPSYRGRGPSMERPLRQLLVFFLVL
                     FGACAGIVALTVAQFMSPIRRLEQRADAMARGELADPVVVAGEGDEIGRLTLALEEMR
                     RALRDKLRSTEEVNLDLERAVQMRTADLARKNRELAETLDKLTRAQEQLVRSEKLASI
                     GQLVAGIAHEINTPVNAIVNTVGPLEQAIDDIDSADAAVRGDAARDVREMVRVVQRGA
                     QRTKAIVGALHNYSRTDDESVVDFDIDRSIDDSLELLRHLLKDKTVTKRYGDVGRVRG
                     HAGQVNQVFMNLLTNAAQALSGRPDANITIETTGDADAVEVRIKDNGAGIPPDVLPRI
                     WDPFFTTKDVGEGTGLGLSIVHELVERHGGTIECHTTVGAGTTFTVKLPRQIPVTAKA
                     RSAKPTGPLVSRSA"
     gene            8612..>8802
                     /locus_tag="VF516_29075"
     CDS             8612..>8802
                     /locus_tag="VF516_29075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111833.1"
                     /translation="MRSTCTALLLVTLVAGCKSKKPDDSAATPDKPSGSASGATKPAD
                     DMKPEPVGKDPGKPAGDTGS"
BASE COUNT         1275 a         3099 c         3054 g         1374 t
ORIGIN      
        1 cgatcgatca tcgaccagga tcttcgacgg caccgccccg gcgacctcgt tgctcgccgc
       61 gggggtgttc ggggccgggg gcatcccggg ctgcggcggc acgttgggcg gccgccgccc
      121 gcccgccccg gccgccgcgg ccgccgccga gatcccgagg atgtcgttga gcttctgcgc
      181 gagctgggtc gcgtcggcgt ggtggatcgg gatcgtgtag atcccgtcgc tgccgcccgg
      241 cacgtcgatc gagcgcgcga gcgacatcat gtcgcggacc tggctggcgt agtcggtgat
      301 gatcagcatc gtgccggcga cctgcacgtt gccggcgggc gaccgcatcg agtcgagcgc
      361 ctggcgcatg atctcgacct gggcgtaggt cggccgcagc acgtagcgca cgatctggtc
      421 ctcgtcgctc ggcacgccgc gcttgtagat cggcacggtc tcgctcttgg cgatcgccga
      481 ctcgacgatg cgcagcgcgt tgcccttggg caccaccgtg agccccatcg tcgacagcgc
      541 gaccaggaac agccggtagg cctcggtcgg ggtcatcttg ttgggcgcga tcagcgtgac
      601 cttcttgccg gtcgacacga tgcgcggctc gaagatgaag ttcttgcacg tgaaccccat
      661 cacccacgtg atcaggtcct tgagctcggt ctcgggcttg aacgtgaccg agacctgccc
      721 ggtcttcagc tgcttgcagt tgtagagctt gtcatcctcg cccgccgccg gcgtcccggc
      781 ggcgccgggc gtgccgggcg ttgtgttcgc gaccggcgtg gtcgccggct gcgccaggcc
      841 gacccgcggt acggacgctc cggtgacgag gccggcgatc acgacgaccg cgacgagtga
      901 acgtgtggtc tgctgcatga ctctcacttg atcgagtact tcagagtgac gggcttgccg
      961 cgacgcgtga cctcgacttc gagcgaggtc gcttcgcgca acttggtgta gacctccagc
     1021 gccttgtccg ccgaggtcag ctcgaagccg ttgatcgact gcagcgtgtc gccgttggcg
     1081 agcccgagct tggcgaacgc cgagctcggc cggatcgcgt acagcttgaa gccgtccggc
     1141 ttgccgttct tcatcgacgg cacgacccgc gcgcccttgg cgaccgccat cgggttgagc
     1201 aggaccttct cgaccagcga cttgtcgatc tcgtagttgt tgtcgtcgag cttcttgatc
     1261 ccggagtcga ccgcggcctg catctcgtcg ccgtcgccgg tgggcaacgc gggcggcgcc
     1321 tcggcggccg cgaccggcgt gaccggcggc gtcgcgccga gcagcaccag ccgctcggtg
     1381 cggccgttgt tctcgaagtc gatgtacttg aagtggatcg ccttgagctt gccggtcgcg
     1441 ccggggatct ggtcgccgac cgagaacgcc ccctgccgct ggttctcgct gttgatgatc
     1501 gtcgcgtacg agtcctcggg ccttggcccg atgttggtcg ccagcagcgc gagcggcagc
     1561 gaggtctgcg cgatctgcga cgggtcggac gactgggtgc tcaccgccgg tgtgcagtcc
     1621 gcgcagaaca tgttgcggct ctggaactgg ccgccgtcct tgctgcgcac cgccgcgacc
     1681 ggcgcctggg tctgtgccac caccggcgtg atcttcggcg cgtgctcggg gtcgcccagg
     1741 aacttggcct cgacgatgtg gttggtggcc ttggccgcga acacggcgca gaccaccacg
     1801 gtgaccgcac cgagtaccca gaagtggcgc ttgatcaggt cctgcatcgg ctagtcttcc
     1861 tccgcgacgg cgccatccgt ggctccgggc gtttcggtct cggcggtctc gggcagctcg
     1921 gaaccttcgc gcgcgtcgcg cgccgcctcg ggatcggtgg tctccagccg gcgatagatc
     1981 gtgcgggtcg cgatcccgag cagcttcgcg gtcagccgct tgtcgccgcg cgtgtggcgc
     2041 agcgtctcga ggatcacccg catctcgatc tcggcgagcg gcatcccgat cgggaacgtc
     2101 agcgacgacg ccgacgcggc gcccggcgtc gcatcgcgga tctcgcgggg cagcgcgtcc
     2161 tcgtcgatca ccgagctccc ggcgcgggtc agcaccaccg cgcgctcgat cgcgttctcc
     2221 agctcgcgca cgttgcccgg ccagccgtac tcggtgagcc gctcgagcgc cgccggcgag
     2281 cagcccgcga tcgccttgcc gttcttctcg gcatacacct gcacgaagtg ctgcgccagc
     2341 agcgagatgt cgtcgcgccg gtcgcgcagc ggcggcaccg acaccgggat cacgttgagc
     2401 cggtagtaca ggtcctcgcg gaaccggccc gccttgacct cctcggccag gttgacgttg
     2461 gtcgcggcga cgatccggac gtcgatccgg cgcgacttgc cgctgccccc cagccgctcg
     2521 atctcgccct cctgcagcac gcgcagcagc ttgacctgca cgtggcgcga gatctcgccg
     2581 atctcgtcga ggaacagcgt cccgccgttc gccgcctcga accgcccgtc gcgcgccgtc
     2641 gccgccccgg tgaacgcccc cttctcgtag ccgaacagct cggcctccag gatcgactcc
     2701 gggatcgccg cgcagttcac cgcgacgaac ggccccggcc cgtcgccctg ccgcccgctg
     2761 cgcgcccgcg cgctgttgtc gtgcagcgcg cgcgccagga gctccttgcc ggtcccgctc
     2821 tcgccgagca gcagcacggt cgcctccgac ggtgccgcct gcatcacgat gtccatcgtg
     2881 cgccgccacg ccagcgacgt cccgatgatc gcgcgccgcc gcttctcggc cagctgcgcc
     2941 ttcaggctgc ggttctcgac cagcagcgac tgcttctcga gcgcgttgcg cacgatccgg
     3001 accacgtgcg cccgcttgag cggcttggtc acgaagtcgt aggcgccctc cttcatcgcg
     3061 tcgaccgcgg tctcgaccgt gccgtacgcc gtcatcagca cgacctcggt ctccggcgcg
     3121 atcgtcttgg ccgcgcgcaa caggtccatg cccgacgtgt tcggcatcat cagatccgtg
     3181 agcagcacgc cgacccggtg cttgcgcagc acgtcgagcc cggccgtccc atcggtcgcc
     3241 gacagcaccg tcagcccctc gcgcttgaag atcttggtca gcgattccac gatcgccggc
     3301 tcgtcatcca ccacgagcac gatcccgccg tccgcggtcc gggccgcctc ccccgggtct
     3361 tccggggacg acatcccgcc ggcgccatcg acctgtgcgg ccgcaaaccg gcgaaatggg
     3421 ccctgtgcag gctggttcca catactcgaa cggttcgtat aacaagcgcc aggccagaac
     3481 cgattccagc taacctgctg aacattctag gcccgaccgc ttgcgaaagc gcccggccgt
     3541 gccagcgtgt caggtcgccc cagccaccct gccaaactgg caagccagcg ctctccaggt
     3601 tcttgctatc tgtacatacg tacagcacga gcaccgagct atgttacgag gatgaggttg
     3661 ccgttcgacc gcgagcgcat gcgtgagcgg aacctgctcg acgacatcgg cgagatcgag
     3721 ctagcagccg cccagtcgcc agcggagcgt ctcgcggcga cgctggctct gagtgacctg
     3781 accctcgcgt tctttcgtgg aaacccggac gcaccggttc tagaccgact cgaggagctc
     3841 gaggagaaag cccggctctg ggccgcgcca ctgagggcaa tccggcggtg acagagctgc
     3901 gacccgcgct cgatgccgcg ctcgatgcgc tcgaggggct cggtcttcgt tatgcggtgg
     3961 tcggcgggct cgcggtcggt gcctggggcg tgccccgcgc tactaaggac gtcgatctgt
     4021 acgccgagct ccccactgcc gtgcgcccgc gggtgcgacg cgagctcatg cagcgcaact
     4081 tcgacgtccc ggccatggac gaggaactcc agcgattcgg cgtgttccgg tcgctgttcc
     4141 gtccgacgag cgtcttcgtg gatatcttcg acgccgagaa tccactcggg gaagcgatct
     4201 tgaaccgtcg gcgcgaggtt caggtacagg gccggatacg ctggacggcc gccgcggagg
     4261 atctcgcggt cctcaaggca ttctccgatc gcgcccggga cctgttctgc ctcggctgga
     4321 acgtgatcgt cggtggcgag agcggcgccg gcgcaaggcc gctcgacaag caatgggtgc
     4381 gctcgttgcg cgatcagtgc gcggcagcca aggtcccgtt cttcttcaag cagtggggcg
     4441 gctttcagaa ggggaagcac ggccgacacc tcgacggccg aacgtacgac gagatgccca
     4501 cgatcagtgc ccggagcgtt cccggcagcg cggagcgaac gcagcgaatt gtcagccttg
     4561 gtgagttcgc cgcagcctgg aagaacgtcc agttcggcgc gccgcggctg ggcagccgag
     4621 cggtttcctg acgccacgcc gtcaccgcca gccgagcccg cccggggtgc ggtcatcgac
     4681 ggcattggtc atcgcgtcga gccagcgcgc ggtggcctcg gcgccgtccg gcaggtcgat
     4741 cgaccacgcc agccctaggt cttcacggcg ccattcgatc agatccgatc tcttatgtcg
     4801 atccggaatc gaccgtcgag gctccgtcga cgcgcgctcg cgagacgcca acgcgtggct
     4861 cgccggggcg accgcgagcg gatggggtcg gacgccgcga ccgtgattac gattggccgg
     4921 gccgtgctcc gcacatacct agcggggtcc gcgcaggacg ggtggtgatt cggcgggcgc
     4981 ggctccgaca cccagccgcg cagcagcccc ggcgagcccg cgtgtcggcg ttctcccggg
     5041 cgctatgtcc tacggactcc tagcgctagc gaagcagcga tttgccagcg ctgtcgagga
     5101 tacggtcata gatgtcggca accgggatcg atgtagacag cgtcggcaac tcgaaactat
     5161 caccgtcacg gtaggtcgtg acgccccatt cgccaccgct tcggcgatag accttcacac
     5221 gtcgctgatc ttgcgcgacg agcacgtagg cttcgagcga ggcaagagac tggaagtcgg
     5281 accgtttctc gccgtcgtca tcgccttcgc tgctgggcga cagcacctcg atcaccatgg
     5341 tcgggttggt ggtagcttga gcgtcgtggg ccggatgctc gggcggacca cagatgatcg
     5401 atccatcggc gtagcggccc cgggcgcgcg atgcgatcca gaaacgctgg tcggatggat
     5461 aatatccgca gcttccggtc tctcggctgc cgagcagctg cgcaagtcta ccggcgatgc
     5521 gattgtgctc atcggacccg cccgccatgg ccacgatcac accgtcgatg aattcgtgac
     5581 ggcattcgct ggtttgctcg accgccaggt agtcagcgta gctcgctcga cgtggcagcc
     5641 ggagggtcgc ttgcatgatt tcaacgtagc acggtcagcg gagggcttcg gcgccgatct
     5701 ggctcgggcg ctcgatgcag cctcacgccg agcgcgacac cagcgggccg gtcggcttcg
     5761 cgctgcgggc cttggcggtg accgggatct ggcgcggcag cttgacggtg aacgtggtgc
     5821 cggcgccgac cgtggtgtgg cattcgatcg tgccgccgtg gcgctcgacg agctcgtgga
     5881 cgatcgacag gccgaggccg gtgccctcgc cgacgtcctt ggtggtgaag aacggatccc
     5941 agatccgcgg caggacgtcg ggcgggatgc cggcgccgtt gtccttgatc cggacctcga
     6001 ccgcgtcggc gtcgccggtg gtctcgatcg tgatgttcgc gtcgggccgt cccgacagcg
     6061 cctgcgccgc gttggtgagc aggttcatga acacctggtt gacctggccg gcgtggccgc
     6121 ggacccggcc gacgtcgccg tagcgcttgg tgacggtctt gtccttgagc aggtggcgga
     6181 gcagctccag cgagtcgtcg atcgagcggt cgatgtcgaa gtcgaccacg ctctcgtcgt
     6241 cggtccgcga gtagttgtgg agcgcgccga cgatcgcctt ggtgcgctgg gcgccgcgct
     6301 ggaccacgcg gaccatctcg cggacgtcgc gcgcggcgtc gccgcggacc gcggcgtcgg
     6361 ccgagtcgat gtcgtcgatc gcctgctcga gcgggccgac cgtgttgacg atcgcgttga
     6421 ccggggtgtt gatctcgtgc gcgatgccgg cgaccagctg gccgatcgac gcgagcttct
     6481 cggaccgcac cagctgctcc tgggcgcggg tcagcttgtc gagggtctcg gccagctcgc
     6541 ggttcttgcg cgcgaggtcg gcggtgcgca tctggaccgc gcgctcgagg tcgaggttga
     6601 cctcctcggt cgaccgcagc ttgtcgcgca gcgcgcggcg catctcctcg agcgcgagcg
     6661 tgagccggcc gatctcgtcg ccctcgccgg cgaccaccac cggatcggcg agctcgccgc
     6721 gcgccatcgc gtcggcgcgc tgctcgagcc ggcggatcgg cgacatgaac tgggcgacgg
     6781 tgagcgcgac gatgccggcg cacgcgccga acagcaccag gaagaacacc agcagctgcc
     6841 ggagcgggcg ctccatcgac gggccgcggc cgcggtagct cgggtagaac accgcgaccg
     6901 cgccgagccg gtacctggcg ccgcgccagc cgatctcgag gatctgggcc cggccggtga
     6961 gctcggcagc gtggatcgcg agcagctcgc cggtcgcgcg ctgcagctgg tagcgcacgt
     7021 accacggcgg ccggggcgcc cccagcccac cgccgccgac cgcgagcaga tcgccgtcgt
     7081 cgtcgaggta gtacatcacc ggggcatcgg gcgccgaggt cgtgatccgg cgcagcgcgc
     7141 cgcgcacccg gtcgggcgcc ggcgccaccg ggtcggagcc gagcgcgcgc tgcagctcgg
     7201 agtggatcca ctgcaggccg agccgcgatt gctggccggc cgcctcggtc gccagactct
     7261 tgtactggac gaagccccac agcagggcca tcccgcacgc cagcagcacg accccgccga
     7321 acgacaagag cagcttggcg cgcagcgaca gcgacggcgc gatgctgcgc accggcacgc
     7381 ggtagcgctg ggtcaggtgg gcgagcagcg gccggagcac gtcgcggtgc cacagctgct
     7441 cgtagatcgc cccggcgatc gcgagcacgg tcatcgcggt ggtcaggacg atcgtgtcgt
     7501 cgagctcgaa cccgaggtgg cgcgcgatga acccgccgat caccgcgatc accagccaca
     7561 ccgcgatcgt catcaccgcg agccggtacg gcagcgcctg ggcgcgccgg tagatcaccg
     7621 ccgccggggg cgcgccggac acgatctcgt tgcgcgacgc cgcgaggtag cgccgcaggt
     7681 cgcgcgtggc catccgggcg accaccagcc acgcggtgac cccggccgcg ctcgcgaccg
     7741 cgacgtaggt ctcgaactgc aggtactgcg ggaacgtgat cggcacgaag tagtaggcga
     7801 acgcggcctg cgcggcgagc acgccgccgg cgacgatcat ggcgaccagc aggaaccgcc
     7861 ggaagtggtt gtcgtcgaac cgcttgagcg gcgagccggc ggcgaacagc cgcgcgctga
     7921 gctcgccgag ggtctgcgcc caccacaccg cgcggatgca gctcaggatg taggcgtgga
     7981 tcgccgcgag cgcggtgatc tcggcgaccc gctcgatcgg ccagtcgcgg aacacgtgga
     8041 ggaacaggcc gatcagcgcc gccgacgccg cccacaggcc ggtgcgcagg agcagcacgc
     8101 ggatcggacc gttgagcgtg atgcggtagg cgcgcgccgc gagctccttg tggacccgct
     8161 cgccgcggcg gcgcgccgcg accgccgacc acagcggcat gagccaggcg gtgatcgccg
     8221 ccagccagac cacgaccgcg ccgcccccga ccggcagcac cgtgcgcagc agcgtgtcgc
     8281 cgccgaaccc ggcgaggtcg aacgcgaccg ccatcgggta cgcgatgaag accagctcgg
     8341 cgacgccgag caccgccgcc gggacgacga gccggcgctt gaacggcagg tcgccgaacg
     8401 gcaggccgga cggccccgag ggcgcgcccg cggggccggg atcgctgatc gccgacgagc
     8461 tccggctggg gccggcgtcg gcgccctgga gatcgtcggt gccagacatg cgggtgggag
     8521 caactcccgc agcgcatcat acccccaacc ccgtggctgt gcaggccggc gtcttgcagg
     8581 ctccgggcaa ctgcgccatg ttccgggcgc catgcgaagc acatgcactg ccctcttgct
     8641 cgtgaccctg gtcgcgggtt gcaagtccaa gaagcccgac gacagcgcgg ccaccccgga
     8701 caagccgagc ggctcggcga gcggggccac gaagcccgcc gacgacatga agcccgagcc
     8761 ggtcggcaag gatcccggca agcccgccgg cgacaccggg tc
//
LOCUS       DASVOR010001544         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_277914,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001544 DASVOR010000000
VERSION     DASVOR010001544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277914"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..887
                     /locus_tag="VF516_29080"
     CDS             <1..887
                     /locus_tag="VF516_29080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111834.1"
                     /translation="AAAPKGPVGERAQLLREAADQLDKAQAALDSGNRNLAEQLFSTA
                     ELLVGADLLAPIAPKFRDGAPPRVTTPTQKVASAPPQPRVAGSSEAEDEQARVPPPRV
                     EASLTGTLEIDGRPASGAFGLITLEPADGRWKPRTPKHVVLEQRNREFLPHVMAVSVG
                     STVAFPNFDPVFHNVFSTSPAAPFDLGLYKSNEAREFTFPKEGIVRLGCNLHANMSAF
                     IAVVAAPAYVVTEGDGRFAFRRLPPGRYKLKAWSERSAAPITQDIAVKPGKNTVTVGV
                     TGDAPRGPQPDKFGGKRG"
     gene            880..2811
                     /locus_tag="VF516_29085"
     CDS             880..2811
                     /locus_tag="VF516_29085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111835.1"
                     /translation="MARPAAVAGAIAIALGGALIAYAVTRSGSGGMSETQTAAMAGAI
                     AKLDGAISAARAAAHERASTLSKLLPVRATIGSNSATVADQVKRGELEFAPGEGEILE
                     LAQVKKAPDSKIEELMVQPVGAMHNMHGGAAGSYADVVDHQLVITEVAEVVPKYTQDV
                     YRGFLTVSRPVSLGPALAPLLESQLTGRLEVGGESIPIGKMPDGAITHGQALGSQGDV
                     KLVVAEPPPRAGLPLPVLVGGIGGVVIGLLLLVVSLLGRRDARTTTGFQALPTRPTGP
                     PRTQLGNAATQLSQHGVPAAGTPVPGGSIAGSPGDSQIVASNLGPGAMIGRWEVVRRL
                     GSGGMADVYLAQARGDGGFEKLVAIKVMHSHLARSQRAVDHFLDEARLAARIHHPNVV
                     AIQDLGKIGNDYVIVMEYVEGVDLERLLMSTRAGQRPVPLAVALGILCRICDGLDAAH
                     RATAPDGSPLGIIHRDVKSANVLVSRQGSVKVVDFGIAKAAKQVHFTVAGETKGTPAM
                     MAPEQRVGEQVDVRADVYSVAAVGYELVTGQAVNLDLASLAHLGVENWPHLPLPSSLR
                     PGMPVELDEILLRAMAFERERRPADCAAFGALCEAVMKRYNLVASDKDLARWVDGELR
                     LLSPAFVGASTGFSRSSPA"
BASE COUNT          431 a         1178 c         1057 g          388 t
ORIGIN      
        1 ccgccgcggc gccgaagggc ccggtgggcg agcgcgccca gctgctgcgc gaggccgccg
       61 atcagctcga caaggcgcag gccgcgctcg acagcggcaa ccgcaacctc gccgagcagc
      121 tgttctcgac cgccgagctc ctcgtcgggg ccgatctgct ggcgccgatc gccccgaagt
      181 tccgcgacgg cgccccgccg cgggtcacca cgccgaccca gaaggtcgcg agcgcgccgc
      241 cgcagccccg ggtcgccggc agctccgagg ccgaggacga gcaggcccgc gtcccgccgc
      301 cgcgggtcga ggccagcctg accgggaccc tggagatcga cggccgcccg gcgagcggtg
      361 cgttcggcct gatcaccctg gagccggccg acggccggtg gaagccgcgc acccccaagc
      421 acgtcgtcct cgagcagcgc aaccgcgagt tcctgcccca cgtgatggcg gtctcggtcg
      481 gatcgaccgt cgcgttcccc aacttcgacc ccgtgttcca caacgtgttc tcgacctcgc
      541 cggccgcgcc gttcgacctc gggctgtaca agtccaacga ggcccgcgag ttcacgttcc
      601 ccaaggaggg catcgtccgg ctcggctgca acctgcacgc gaacatgagc gcgttcatcg
      661 cggtggtcgc cgcgccggcc tacgtcgtca ccgaaggcga cggccggttc gcgttccggc
      721 gcctgccgcc cggcaggtac aagctcaagg cgtggagcga gcgcagcgcc gcgccgatca
      781 cccaggacat cgcggtcaag cccggcaaga acaccgtgac cgtcggcgtc accggcgacg
      841 ccccgcgggg tccacagccc gacaagttcg gaggcaagcg tggctaggcc cgcggccgtc
      901 gccggtgcga tcgccatcgc gctcggcggc gccctgatcg cctacgcggt cacccgctcg
      961 gggagcggcg gcatgtccga gacccagacc gccgcgatgg ccggcgcgat cgccaagctc
     1021 gacggcgcga tcagcgcggc gcgcgccgcg gcccacgagc gggccagcac gctgtcgaag
     1081 ctcctcccgg tgcgtgcgac gatcgggagc aattccgcga ccgtcgccga ccaggtcaag
     1141 cgcggcgagc tcgagttcgc gcccggcgag ggcgagatcc tcgagctggc gcaggtcaag
     1201 aaggcgccgg acagcaagat cgaggagctg atggttcagc ccgtcggtgc gatgcacaac
     1261 atgcacggcg gggcggccgg cagttacgcc gacgtggtcg accatcagct cgtgatcacc
     1321 gaggtcgcgg aggtcgtgcc caagtacacg caggacgtct accgcgggtt cctcacggtc
     1381 agccggccgg tctcactcgg cccggcgctg gcgccgctgc tcgagtccca gctcaccggc
     1441 aggctcgagg tcggcggtga gtcgatcccg atcgggaaga tgccggacgg cgcgatcacc
     1501 cacggacagg cgctgggctc gcagggcgac gtcaagctgg tcgtcgccga gccaccgccg
     1561 cgcgccggcc tgccgctgcc ggtcctggtc ggcggcatcg gcggcgtggt gatcggcctc
     1621 ctgctcctcg tggtcagcct gctgggtcgc cgcgacgccc ggaccaccac cggcttccag
     1681 gccctgccga cccggcccac cggcccgccg cgcacccagc tcggcaacgc cgcgacccag
     1741 ctgtcgcagc acggcgtccc cgccgccggc accccggtcc cgggaggttc gatcgccggc
     1801 tcgccgggcg attcccagat cgtcgcatcc aacctcggcc ccggcgcgat gatcggccgc
     1861 tgggaggtcg tccgccggct cggctcgggc ggcatggccg acgtctacct cgcccaggcc
     1921 cgcggcgacg gcgggttcga gaagctggtc gcgatcaagg tgatgcacag ccacctcgcg
     1981 cgcagccagc gcgccgtcga ccacttcctc gacgaggccc ggctcgccgc ccggatccac
     2041 caccccaacg tcgtcgcgat ccaggacctc ggcaagatcg gcaacgacta cgtcatcgtc
     2101 atggagtacg tcgagggcgt cgacctcgag cgcctgctga tgtcgacgcg cgccggccag
     2161 cgcccggtgc cgctcgcggt cgcgctcggc atcctgtgcc ggatctgcga cggcctcgac
     2221 gccgcccacc gcgcgaccgc gcccgacggc agcccgctcg gcatcatcca ccgcgacgtc
     2281 aagagcgcca acgtcctggt gtcgcgccag ggcagcgtca aggtcgtcga tttcggcatc
     2341 gccaaggccg ccaagcaggt ccacttcacc gtcgccggcg agaccaaggg caccccggcg
     2401 atgatggcgc ccgagcagcg ggtcggcgag caggtcgatg tccgcgccga cgtctactcg
     2461 gtcgccgcgg tcggctacga gctggtcacc ggccaggcgg tcaatctcga cctcgcatcg
     2521 ctcgcccacc tcggggtcga gaactggccg cacctgccgc tgccgtcgag cttgcgcccc
     2581 ggcatgccgg tcgagctcga cgagatcctg ctccgcgcga tggcgttcga gcgcgagcgc
     2641 cgcccggccg actgcgccgc gttcggcgcg ctgtgcgagg cggtcatgaa gcgctacaac
     2701 ctggtggcga gcgacaagga cctcgcgcgc tgggtcgacg gcgagctgcg cctgctgtcg
     2761 ccggcgttcg tcggcgcgag caccggcttc agccggtcgt cgccggcgta ggccgccgca
     2821 cgccgctggc aaattcaggc acccacgtgg ggacgccgcg cggctcgcgg tccgtcgcgc
     2881 aagccgcgcg cgccaacacg cgctgtcctg cgacacgtga gctccgctcg acggcgacgc
     2941 aggctcatcg acgtgcgctc cggcccgatc ttgccgcttg ccgcgtcgca agtcgcgcta
     3001 cggtgtcgga accgagaccg ccgatgtcga ccccgaagag ccgcgtcccc cgtg
//
LOCUS       DASVOR010001545         2698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_249888,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001545 DASVOR010000000
VERSION     DASVOR010001545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249888"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF516_29090"
     CDS             complement(<1..216)
                     /locus_tag="VF516_29090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111836.1"
                     /translation="MTHLRIVTLNLWGTEPPLDARLALAARQLATLAPDAVCLQEVRP
                     LDGRTGRTTAEVLADALGMTAHYETAVA"
     gene            complement(254..616)
                     /gene="queD"
                     /locus_tag="VF516_29095"
     CDS             complement(254..616)
                     /gene="queD"
                     /locus_tag="VF516_29095"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010434567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEX8111837.1"
                     /translation="MSTRLERSYRFEAAHFLPRVPPGHKCARMHGHSYLIDVAIEGEL
                     DPERGWVMDFAEIDEQVAPLVRQLDHQVLNEIEGLTNPTSELLARWCWQKLAPRLPGL
                     CELVISETPTSRCIYRGR"
     gene            697..1932
                     /locus_tag="VF516_29100"
     CDS             697..1932
                     /locus_tag="VF516_29100"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8111838.1"
                     /translation="MLKLGPRAGLDRRVWQMAVARAVSTMGMSLVMSFLGIYVVETRG
                     YPAWVYGVIALCANAGQSLSNAWAGNLSDRIGRRPLITNALFVRAGVIALLGTQILLD
                     APLWTLGANMVIASTLRGCFEPVAYALVADVVRDDQRIAAFGIQRMGTNVGWAVGPAL
                     GGVLSLVMPYGAVFYFAAAGLVLTGFLTLRVEDPARQAPAAAPSRSQDLLDALFEGLA
                     DRVMSMLLIGTFLCQLLETQMFTTFGIYMTTQLGLTKAEFGLLYTINGAVVLVLQVPA
                     LAVIRRIGIGVVLPWSSFLDALGFAVIGLATGFAGGALSIVILTCAEVLFDPAQQAAI
                     AEVADPARRGRAFGVVGFASMIGIAFAPLLGGRLLDAIGTHHVAMWLAIAAIGAGQTL
                     CFIAFVRRRTQVRPLNAVP"
     gene            2078..2491
                     /locus_tag="VF516_29105"
     CDS             2078..2491
                     /locus_tag="VF516_29105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111839.1"
                     /translation="MRFAGLALMACLAGCSLTPGTGGECVNDSQCGDDVCTRGGECVA
                     RANVRAVMVKWTVEGAAASATTCGAHPDLYLQFNGADYGDTLRFEPVACRQGSYLIDK
                     LPKRYQQVELGIQGGTGTALTIDPASAQAMFDLFQ"
BASE COUNT          341 a          960 c          951 g          446 t
ORIGIN      
        1 cgccacggcg gtctcgtagt gcgcggtcat gccgagcgcg tcggcgagta cctcggcggt
       61 ggtgcggccg gtgcggccgt cgagcgggcg gacctcctgc aagcacacgg catcgggtgc
      121 cagcgtcgcg agctggcgcg ccgcgagcgc gagccgcgcg tcgagcggcg gctcggtgcc
      181 ccacaggttc agggtcacga tgcgcaggtg ggtcacgcgg tcacgctacc ccgccggccg
      241 accggtacac agctcaccgg ccgcgataga tacagcgcga ggtcggggtc tccgagatca
      301 cgagctcgca cagcccgggc agccgcggtg caagcttctg ccagcaccag cgcgcgagca
      361 gctcgctcgt cgggttggtc aggccttcga tctcgttgag cacctgatgg tcgagctggc
      421 ggaccagcgg cgcgacctgc tcgtcgatct cggcgaagtc catcacccag ccgcgctcgg
      481 ggtcgagctc gccctcgatc gcgacgtcga tcaaatagct gtggccgtgc atgcgtgcgc
      541 acttgtgtcc cgggggcacc ctcgggagga agtgcgccgc ctcgaaccgg taactgcgct
      601 ccagccgcgt cgacatcgcg cccggtttgt agcgcgccgc gatgtggtag gaaagcaggc
      661 gatggtcctg ggtgacccga gggtgcacgc gccacgatgt tgaagctcgg cccgcgagcg
      721 ggcctcgatc gtcgtgtctg gcagatggcg gtcgcccgcg cggtcagcac catgggcatg
      781 tcgctggtga tgtcgttcct cgggatctac gtcgtcgaga cccggggcta cccggcgtgg
      841 gtctacggcg tgatcgcgct gtgcgccaac gccggccagt cgctgtcgaa cgcatgggcc
      901 ggcaacctgt ccgaccggat cgggcgccgg ccgctgatca ccaacgcgct gttcgtgcgc
      961 gcgggggtca tcgcgctgct cgggacccag atcctgctcg acgcgccgct gtggacgctc
     1021 ggcgccaaca tggtgatcgc ctcgacgttg cgcggctgct tcgagccggt cgcctacgcg
     1081 ctggtcgccg acgtcgtgcg cgacgaccag cgcatcgcgg cgttcggcat ccagcggatg
     1141 ggcaccaacg tcggctgggc cgtcggcccg gcgctgggcg gcgtgctgtc gctggtgatg
     1201 ccgtacggcg cggtgttcta cttcgccgcc gcgggcctcg tgctcaccgg gttcctcacg
     1261 ctgcgcgtcg aggacccggc gcgccaggcc cccgcggcgg cgccgtcccg ctcgcaggac
     1321 ctgctcgatg cgctgttcga gggcctggcc gaccgcgtga tgtccatgct gctgatcggc
     1381 acgttcctgt gccagctgct cgagacccag atgttcacca cgttcgggat ctacatgacc
     1441 acgcagctcg gcctgaccaa ggccgagttc ggcctgctct acacgatcaa cggcgcggtg
     1501 gtgctcgtgc tccaggtccc ggcgctcgcg gtgatccggc ggatcggcat cggggtcgtg
     1561 ctgccgtggt cgtcgttcct cgatgcgctc ggcttcgcgg tgatcggcct ggccacgggg
     1621 ttcgccggcg gcgcgctctc gatcgtcatc ctgacctgcg ccgaggtgct gttcgatccg
     1681 gcgcagcagg cggcgatcgc cgaggtcgcc gatccggccc gccgcggccg cgcgttcggc
     1741 gtggtgggct tcgcctcgat gatcgggatc gcgttcgcgc cgctgctcgg cgggcggctg
     1801 ctcgacgcga tcggcaccca ccacgtggcg atgtggctcg cgatcgcggc gatcggcgcc
     1861 ggccagaccc tgtgcttcat cgcgttcgtg cgtcgacgca cgcaggtccg accgctgaac
     1921 gccgtgccgt agtgggctgc accccccact cccgcgccgg cgatcccggc tatgtcagtg
     1981 atcgcgcgcc gcggcaagct ccggtgagct tcacttcgcg gtgcaacgcg gtgcgatcta
     2041 cgtgcaggaa cccttcggtt ttgctaaaca agactccatg cggttcgccg gtctcgcgct
     2101 gatggcctgc cttgcgggct gttcgctgac gcccggtacc gggggcgagt gcgtcaacga
     2161 ttcgcagtgc ggcgacgacg tctgcacgcg cggcggcgag tgcgtcgccc gggccaacgt
     2221 gcgcgcggtc atggtgaagt ggacggtcga gggcgcggcg gccagcgcca cgacctgtgg
     2281 ggcccacccc gatctctacc tccagttcaa cggcgccgac tacggcgaca ccctccggtt
     2341 cgaaccggtc gcgtgccgcc agggctcgta cctcatcgac aagctgccca agcgctacca
     2401 gcaggtcgag ctcggcatcc agggcggcac cggcaccgcg ctgacgatcg acccggcctc
     2461 cgcgcaggcg atgttcgatc tgttccagtg atccagtccg gatcgcccgg cgcagtccgg
     2521 ctcgatccgg ctccgtcccg gcgcagtccg tcccggcgca gtccggccgg cgcagtccgg
     2581 ccggcgcagt ccggggaccg tctggcgttc cgacacaggt cttcagcgcc tcagagatcc
     2641 gacagctccc cgatccggct gcccagatcc ggctgcccag atccggctgc ccagatcc
//
LOCUS       DASVOR010001546         1607 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_74070,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001546 DASVOR010000000
VERSION     DASVOR010001546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74070"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..476)
                     /locus_tag="VF516_29110"
     CDS             complement(108..476)
                     /locus_tag="VF516_29110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX8111840.1"
                     /translation="MPSFQRHVFVCINERAPGNPKGCCKWKGGVDVRDRLKAELSARG
                     LSKVVRANNAGCLDQCEAGVTVVVYPEQVWYGRVTPDDIPEIVEKHLIGGQVVARLLL
                     PDQPHLGGRTSFPAIEIGAR"
     gene            631..>1607
                     /locus_tag="VF516_29115"
     CDS             631..>1607
                     /locus_tag="VF516_29115"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111841.1"
                     /translation="MVLGSYRILHELTSGGMGTVFRAEHVLLGRPAAVKLLRRDLSTS
                     PALLQRFVNEAKAVTACKHPGIVEVYDFGYTPDGHAFIVMEFLDGESLGRRLARLRLT
                     EVEATAIAHGIASALRAAHRVGVIHRDLKPDNVFLVADPDGGVDRTKVLDFGIAKLAD
                     AASDSRTQTGALIGTPLYMAPEQARAAAAIDHRADLYSLGCILYHMLTGRPPFVAEGA
                     GEIIALQMFGEVVPPSRLAPVSPEMEHVVLRLLDKDPDQRFPSAGELASALTHLDRRP
                     THLMTRASQPALGDPGATRPGGVAVEPGEPAAASSVLVLPDPRTTAAVR"
BASE COUNT          225 a          599 c          564 g          219 t
ORIGIN      
        1 gggcgctgcg gacgacgtcg ccgcgggtgg tgtcgccgca ggaggcctcg gctcggcgtg
       61 tgcgacgccg gcgaccgcca gtgcgccggc cacggcgagc cggccgatca tcgagcgccg
      121 atctcgatcg ccggaaacga cgtccgaccg ccgaggtgcg gctggtcggg cagcagcagg
      181 cgggcgacga cctggccgcc gatcaggtgc ttctcgacga tctccgggat gtcgtcgggc
      241 gtgacccggc cgtaccagac ctgctcgggg tagaccacga cggtcacgcc ggcctcgcac
      301 tggtctagac accccgcgtt attggcgcgg acgaccttgg acaggccgcg ggcggacagc
      361 tcggccttga gccggtcgcg gacgtcgacc ccgcccttcc acttgcagca acccttgggg
      421 ttacccggcg cacgctcgtt gatgcagacg aacacatgac gctgaaagct cggcatccgc
      481 cgggtagagt agcatcctca ccctgccgtc cgcggccccc acgtggggga agcttgaagc
      541 ggtcggcgcg gtggcgcatc ctaggggtct cggcccgaat gagcccgaat gagcgatagc
      601 cgcacacgtt ccggcccgac ggccgagggg atggtcctcg ggagttaccg gatcctgcac
      661 gagctgacca gcggcgggat gggcacggtg tttcgcgccg agcacgtgct gctcggccgg
      721 ccggccgcgg tcaagctgct gcgccgcgat ctgtcgacca gccccgcgct gctccagcgg
      781 ttcgtcaacg aggccaaggc ggtcaccgcc tgcaagcacc ccggcatcgt cgaggtctat
      841 gacttcggct acaccccgga cggccacgcg ttcatcgtga tggagttcct cgacggggag
      901 tcgctcggcc ggcggctggc gcggctgcgg ctcaccgagg tcgaggccac cgccatcgcg
      961 cacggcatcg cgagcgcgct gcgcgcggcg caccgggtcg gcgtcatcca ccgcgatctc
     1021 aagccggaca acgtgttcct ggtcgcggat cccgacggcg gggtcgatcg caccaaggtg
     1081 ctcgacttcg ggatcgccaa gctcgccgac gccgcgtccg acagccgcac ccagaccggc
     1141 gcgctgatcg gcacgccgct gtacatggcc cccgagcagg cgcgcgccgc ggccgcgatc
     1201 gatcaccgcg ccgacctgta ctcgctgggc tgcatcctct accacatgct caccggccgg
     1261 ccgccgttcg tcgccgaggg cgccggcgag atcatcgcgc tgcagatgtt cggcgaggtc
     1321 gtgcccccga gccggctcgc gccggtctcg cccgagatgg agcacgtggt gctgcggctg
     1381 ctcgacaagg atccggacca gcggttcccc agcgccggcg agctcgccag cgcgctgacc
     1441 cacctcgatc gccggccgac ccacctgatg acgcgcgcgt cccagcccgc gctcggcgat
     1501 cccggcgcga cccgaccggg cggcgtcgcc gtcgaacccg gtgagcccgc ggccgccagc
     1561 agcgtgctcg tcctgcctga cccgcgcacg accgccgcgg tgcggag
//
LOCUS       DASVOR010001547         2252 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_336965,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001547 DASVOR010000000
VERSION     DASVOR010001547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2252
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336965"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="VF516_29120"
     CDS             complement(<1..878)
                     /locus_tag="VF516_29120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111842.1"
                     /translation="MRGVVLACALAAAAGCRQTPARESGNEASNERVLTFSWVNRGHP
                     TGEAEIRIEPDGKRVTHFAFQDRGRGPDIRTTSIFDPTGALRWFHVTGVDYNRAPVDE
                     RLEESDGVLRWQSPSERGQAPAGAGWYVTAQLNIDPYALLARGLRHAAGHRLKLLPAG
                     EAWIDDDTTREFQVAGVARHLRRVAIAGLDYTPQVIWLDEDDEMFAGGDTAFGAIRTG
                     AEAVLPALEADNLAWSSARAARLAQQLGHRPPGGKLAFIHARLYDSERRTVVPDATVV
                     VAGDRIAAVGGAATPVP"
     gene            1117..>2252
                     /locus_tag="VF516_29125"
     CDS             1117..>2252
                     /locus_tag="VF516_29125"
                     /inference="COORDINATES: protein motif:HMM:NF006488.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEX8111843.1"
                     /translation="MKTGNATLSGYGTTIFEAMSALAREHAAVNLGQGFPDDRGRLDL
                     RRAAADYVVDGHNQYPPMMGMPALRQAVAAHDRRFYGLEVDWQTEVLVTSGATEALFD
                     CFLGLLEPGDEVVVLEPAYDTYVPVIRRLGAIPRTVRLVPPAWTLPREELAAAFSPRT
                     KLLLFNSPMNPAGKVFTPDELGFIASLVERHDVFAMCDEVYEHLVFGDARHVPLMTLP
                     GMRDRTLRIGSAGKTFSVTGWKVGYITAPAAMLRPITKAHQFVTFTTPPNLQHAVAIG
                     LGLEDAFFQGIAVPLARGRDRLVAGLTSLEVPVLPCDGTYFVVLDVTRWLRPGEDDIA
                     FCRRLVIDAGVVLIPMSAFYEAAPPRHLVRACFCKEDATIDGAL"
BASE COUNT          304 a          784 c          815 g          349 t
ORIGIN      
        1 ggcaccggcg tcgcggcgcc gccgaccgcc gcgatccggt cgccggcgac caccacggtg
       61 gcgtcgggca cgacggtgcg gcgctcgctg tcgtagagcc gggcgtggat gaatgcgagc
      121 ttgcccccgg gagggcgatg cccgagctgt tgcgccagcc gggccgcgcg cgcgctcgac
      181 cacgccaggt tgtcggcctc gagcgccggc agcacggcct cggcgccggt gcggatcgcc
      241 ccgaacgccg tgtcgccccc ggcgaacatc tcgtcgtcct cgtcgagcca gatcacctgc
      301 ggcgtgtagt cgagcccggc gatcgcgacc cggcggaggt ggcgcgcgac cccggcgacc
      361 tggaactccc gcgtcgtgtc atcatcgatc cacgcctcgc cggccggcag cagcttgagg
      421 cggtggcccg cggcgtggcg cagcccgcgc gcgagcagcg cgtacggatc gatgttgagc
      481 tgcgccgtga cataccagcc cgcgccggcc ggggcctggc cgcgctcgct cggcgattgc
      541 cagcgcagca cgccgtcgga ctcctcgagt cgctcgtcga ccggcgcccg gttgtagtcg
      601 acgccggtga cgtggaacca gcgcagcgcg ccggtgggat cgaagatcga ggtggtgcgg
      661 atgtccgggc cgcggccgcg atcctggaac gcgaagtgcg tgacgcgctt gccgtcgggc
      721 tcgatccgga tctcggcctc gccggtggga tggccgcggt tgacccacga gaacgtcagc
      781 acgcgctcgt tgctcgcctc gttacccgac tcgcgcgcag gcgtctgccg gcatccggcc
      841 gcggctgcca gtgcgcacgc gagcaccaca ccgcgcatgc cggcacggtc gcatgaccga
      901 ccgcgcagga caacggagca acgacacgag cgtgatgcgg tcacggtgaa ctcacggggg
      961 agttcatgac cggctcgtga gctcgatgac accgcgtgca ggcacagaac aggtcgactt
     1021 tcactcaaca gcgcatgttc gccgttccat tggtgttctc ggccgcgctc gctcgtcagc
     1081 ttggctgccg cgatgtgcga cggttagact cgcgtggtga agaccgggaa tgccacgctg
     1141 tcaggctacg ggacgacgat cttcgaggcg atgtccgcgc tcgcgcgcga gcacgcggcg
     1201 gtcaacctcg gccagggctt cccggacgat cgcggccggc tcgacctgcg gcgcgccgcg
     1261 gcggactacg tggtcgacgg ccacaatcaa tatccgccga tgatggggat gccggcgctg
     1321 cgccaggcgg tggcggcgca cgaccgccgg ttctacgggc tcgaggtcga ctggcagacc
     1381 gaggtgctgg tcacctcggg tgccaccgag gcgctgttcg actgcttcct gggcttgctg
     1441 gagcccggcg atgaggtcgt ggtgctggag ccggcgtacg atacgtacgt gccggtgatc
     1501 cgccggctcg gcgcgatccc gcgtacggtc cggctcgtac cgccggcctg gacgctgccg
     1561 cgcgaggagc tcgccgcggc gttctcgccc cggaccaagc tgctgctctt caactcgccg
     1621 atgaacccgg ccggcaaggt gttcacgccc gacgagctcg gcttcatcgc gtcgctggtc
     1681 gagcgccacg acgtgttcgc gatgtgcgac gaggtgtacg agcacctggt gttcggggac
     1741 gcccggcacg tcccgctgat gacactgccg gggatgcgcg accgcacgct gcggatcggg
     1801 tcggccggca agacgttctc ggttaccggc tggaaggtcg gctacatcac cgcgccggcc
     1861 gcgatgttgc gtccgatcac caaggcccac cagttcgtga cgttcacgac gccgcccaac
     1921 ctgcagcacg cggtggcgat cggcctcggt ctggaggatg ccttcttcca gggcatcgcg
     1981 gtgccgctgg cgcgcggccg cgatcgcctg gtcgccggcc tgacctcgct cgaggtcccg
     2041 gtgctgccgt gcgatgggac gtacttcgtc gtactcgatg tcacgcgctg gctgcggccg
     2101 ggcgaggatg acatcgcgtt ctgccgccgc ctggtgatcg acgccggggt ggtgctgatc
     2161 ccgatgagcg cgttctacga ggccgcgccg ccacgccacc tggtgcgcgc gtgcttttgc
     2221 aaggaagacg ccacgatcga cggggcgctg ga
//
LOCUS       DASVOR010001548         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_132532,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001548 DASVOR010000000
VERSION     DASVOR010001548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132532"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VF516_29130"
     CDS             complement(<1..191)
                     /locus_tag="VF516_29130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEX8111844.1"
                     /translation="MTADDCPEIERTHALADGELTGDAADAARDHLATCAVCQSELAD
                     VLLLDAAVRAAGHRAPVISL"
     gene            complement(188..700)
                     /locus_tag="VF516_29135"
     CDS             complement(188..700)
                     /locus_tag="VF516_29135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8111845.1"
                     /translation="MTVAERSFSEAVREHEALLTAIARRLCGNDADAADLVHDTYERA
                     LRAWDRYSDRGNFRSWMVAILNNLFIDRCRKHRRTPRTEALDGLELAAPESGAAPVWS
                     QVTAQQVDAALATLGPEFRRVYELHVRGRSYDEIAAELRIAKPTVGTRLIRARKKLKE
                     ALLRDLGGEP"
     gene            841..1026
                     /locus_tag="VF516_29140"
     CDS             841..1026
                     /locus_tag="VF516_29140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111846.1"
                     /translation="MSMACVLIFAAIAAIAPHRPQALPIQARPVRAAIASAGDPLRLP
                     SGYRSVRISLRWQAPER"
     gene            complement(1062..>1573)
                     /locus_tag="VF516_29145"
     CDS             complement(1062..>1573)
                     /locus_tag="VF516_29145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111847.1"
                     /translation="AAAGAQDPAKPGSADPAPDPKPAELAGAEAASDDPKPAGPQTAD
                     GKATDGKAADGKAANRRATDGKTADDKATSGKAADARTGEGRSSGRRSAGARDTNKRR
                     GILAVDASPWSWVLVDVDPQYHETPTKFYLEPGVHTVKLVNDNSGRTRYAKVTIEPDK
                     LSRLDVDMN"
BASE COUNT          183 a          559 c          573 g          258 t
ORIGIN      
        1 agcgagatca ccggggcgcg gtggccggct gctcggaccg cggcgtcgag cagcagcacg
       61 tcggcgagct cggactggca gaccgcgcag gtcgcgaggt gatcgcgggc cgcatcggcg
      121 gcatcgccgg tcagctcgcc gtcggccagc gcgtgggtgc gctcgatctc ggggcagtcg
      181 tccgcggtca tggctcacct ccgaggtcgc gcagcagcgc ttccttgagc ttcttgcgcg
      241 cgcggatcag gcgggtcccg acggtcggct tggcgatccg gagctccgcc gcgatctcgt
      301 cgtacgaccg gccgcggacg tggagctcgt agacccgccg gaactcgggg ccgagcgtgg
      361 cgagcgcggc gtcgacctgc tgcgcggtga cctgcgacca caccggcgcg gcgcccgact
      421 cgggggcggc cagctccagg ccgtcgagcg cctcggtgcg gggcgtgcgg cggtgcttgc
      481 ggcagcggtc gatgaacagg ttgttgagga tcgcgaccat ccagctgcgg aagttgccgc
      541 gatcggagta gcggtcccac gcgcgcagcg cgcgctcgta ggtgtcatgc accaggtccg
      601 cggcgtcggc gtcgttgccg cacagccggc gtgcgatcgc ggtgagcagc gcctcgtgct
      661 cgcggaccgc ctcgctgaat gaacgctccg cgacggtcac agcacgacga cggtcccacg
      721 caggctcgcc ggcggtcaac aacgcggcat ggtccatcca tcccaggaaa cgaccgatcc
      781 gggagatctt cccgacaatc gcgccgcggg aacaatcgtg cgatttcacg tttctcgggc
      841 atgtcgatgg cgtgtgtctt gatctttgcg gcgatcgcgg cgatcgcgcc gcaccgaccc
      901 caggcgctgc cgatccaggc gcgtccggtg cgcgccgcga tcgcctccgc cggcgatcca
      961 ctgcggctgc cctcgggcta ccgatcggtg cggatcagcc tgcgctggca ggcgcccgaa
     1021 cggtagcgcg gaacatccgc caccccgccg gattttccgc gctagttcat gtcgacgtcg
     1081 agccgggaca gcttgtcggg ctcgatcgtg accttggcgt accgggtccg cccgctgttg
     1141 tcgttgacga gcttcaccgt gtggacgccc ggctcgaggt agaacttggt cggagtctcg
     1201 tggtactgcg ggtcgacgtc gacgaggacc cacgaccacg gcgacgcgtc gacggccagg
     1261 atgcccctgc gcttgttcgt gtcgcgcgcg ccggccgatc gccgaccgct ggaccggcct
     1321 tccccggtcc tggcatcggc tgccttgccg gaggtcgcct tgtcgtcggc cgtcttgccg
     1381 tcggtcgccc tgcgattggc cgccttgccg tcggccgcct tgccgtcggt cgccttgccg
     1441 tcggcggtct gcggcccggc gggcttcggg tcgtcgcttg ccgcctccgc gcctgcgagc
     1501 tcggccggct tcggatccgg cgccggatcg gcgctgccgg gcttcgccgg gtcctgcgca
     1561 ccagcggcgg ccg
//
LOCUS       DASVOR010001549         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_453950,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001549 DASVOR010000000
VERSION     DASVOR010001549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_453950"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2049
                     /locus_tag="VF516_29150"
     CDS             <1..2049
                     /locus_tag="VF516_29150"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8111848.1"
                     /translation="SIAMQPGALATELGDPALGGPLAFTIRRGQRLRASGLDVQLGGV
                     LPAGLATGDILIELLSDAGGRIYRNPHQPGDQRPDNDHSPLIADLSLDLAIYAVDAEG
                     NAVLTQTVLGVQGSGVVIADDGVLDIEAAIAIDLDLLGLATTTTNLVLELITDPGAKI
                     DADQTAPMLLAALPGATAAPASLDTAVELVFSEPIDLESARAGGIRLQTAAGQPVPSS
                     IESHGAALAIHPLSPLLPGTTYQVALGDVADLAGNPLPATAAPSFTIPPRIATTAPPM
                     LVSVHPGAPCALTGGGATTPGHCRTSTTAGDDYHPFSLAANDAIDVRFTQSLAPTSVT
                     RGTACNTGSVRIEELDGAGGCVAPIAGTLRLRDRAVSFVPDQPWQAGKHYRLTLVSGA
                     DDSCDAGEICGTNGVAVNLSPLDRGALGALVRANLVSDFTGTAAATATLATMQTVPIG
                     DVNGSGTIDSGEPAIDDNRVALRITGASGVLSSASFPVTDCIPATPEVEACSALSAAL
                     PVALLPAAHDCAIPGGGTAASCVPVALSPQQVVGTSVPLDATLITGQTLSNNNTRDLV
                     MRLREPPDGPAMGYIIDDHGTPTFVATLSLYLDAPGLQIALVDHDLHSKPLSVAVRGP
                     LRFLPDGRIAIAVANVADVPTDVNLLQLGVTVGTVHMVIPRGELKLQLVSPPLRGGAR
                     "
BASE COUNT          297 a          850 c          727 g          290 t
ORIGIN      
        1 tcgatcgcga tgcagcccgg cgcgctcgcg accgagctcg gcgatccggc gctcggtggg
       61 ccgctcgcgt tcacgatccg gcgcggccag cgcctgcgcg ccagcgggct tgacgtgcag
      121 ctcggcggcg tgctgccggc cgggctcgcc accggcgaca tcctgatcga gctgctcagc
      181 gacgccggcg gccggatcta ccgcaatccg caccagccgg gcgatcagcg ccccgacaac
      241 gaccactccc cgctgatcgc cgacctcagc ctcgacctcg cgatctacgc ggtcgacgcc
      301 gagggcaacg ccgtgctgac ccagaccgtg ctcggcgtgc agggctcggg cgtggtgatc
      361 gccgacgacg gcgtgctcga catcgaggcc gcgatcgcga tcgatctcga cctgctcggg
      421 ctcgcgacca cgaccacgaa cctggtgctg gagctgatca ccgatcccgg cgcgaagatc
      481 gacgccgacc agaccgcccc gatgctgctg gcggcgctgc cgggcgccac cgccgccccc
      541 gcgtcgctcg acaccgcggt cgagctggtg ttcagcgagc cgatcgatct ggagagcgcg
      601 cgcgccggtg ggatccggct ccagaccgcc gccgggcagc ccgtgccgag ctcgatcgag
      661 agccacggcg cggcactcgc gattcacccg ctgtcgccgc tcctgcccgg gacgacctac
      721 caggtcgcgc tgggcgacgt cgccgacctc gcgggcaacc cgttgccggc gaccgccgca
      781 ccgagcttca cgatcccgcc ccggatcgcg accaccgcgc caccgatgct cgtgtcggtg
      841 cacccgggcg cgccgtgcgc gctgaccggc ggcggcgcga cgacgccggg gcactgccgg
      901 accagcacca cggccggcga cgactaccat ccgttctcgc tcgccgcgaa cgacgcgatc
      961 gacgtccggt tcacccagtc gctggcgccg acctcggtga cgcgcggcac cgcgtgcaac
     1021 accggcagcg tccgtatcga ggagctcgac ggcgccggtg gctgtgtcgc gccgatcgcc
     1081 gggaccctgc ggctgcgcga tcgcgccgtg tcgttcgttc ccgatcagcc atggcaggcc
     1141 ggcaagcact accgcctgac gctggtctcc ggcgccgatg acagctgcga cgccggcgag
     1201 atctgcggga ccaacggcgt cgcggtgaac ctgagcccgc tcgaccgcgg ggctctgggc
     1261 gcgctcgtgc gagcgaacct cgtgagcgac ttcacgggca ccgccgcggc caccgcgacc
     1321 ctggcgacga tgcagaccgt gccgatcggc gacgtcaacg gctcgggcac gatcgactcc
     1381 ggcgagccgg ccatcgacga caaccgcgtg gcgctgcgca tcaccggcgc gtccggcgtg
     1441 ctgagctccg cgagcttccc ggtcaccgat tgcatccccg ccacccccga ggtcgaagcg
     1501 tgcagcgcgc tgtccgccgc gctccccgtc gccctgctcc cggcggcgca cgactgcgcg
     1561 atccccggcg gcggcaccgc tgcgagctgc gtcccggtgg cgctgtcgcc ccagcaggtc
     1621 gtcgggacct cggtgccgct ggacgcgacc ctgatcacgg gccagacgct cagcaacaac
     1681 aacacccgcg atctggtgat gcgcctccgc gagccccccg acggcccggc gatgggatac
     1741 atcatcgacg atcacggcac gccgaccttc gtggccacgc tctcgctgta tctcgacgcc
     1801 cccggcctgc agatcgcgct ggtcgatcac gacctgcact ccaagccgct gtcggtcgcc
     1861 gtgcgcggac cgctgcggtt cctccccgac gggcggatcg cgatcgcggt ggccaacgtc
     1921 gcggatgttc cgaccgacgt caacctgctc cagctcggtg tcacggtcgg caccgtacac
     1981 atggtgatac cgcgcggaga gctcaagctc cagctggtgt cgccgcccct gcgcggaggt
     2041 gcgcgatgat cccgtcgaac gaagcatcga tcgaagcatc gatcgaagcc catgactgcc
     2101 atcggccgtc cgcggcccgc tgctggctcg ccgtcgcggc gttcgtcgcc gccgcggtcg
     2161 ccgg
//
LOCUS       DASVOR010001550         5520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_249947,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001550 DASVOR010000000
VERSION     DASVOR010001550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249947"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="VF516_29155"
     CDS             <1..281
                     /locus_tag="VF516_29155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111849.1"
                     /translation="EPDASAEHATRGPRAVRDVGPAELTPAFDTAVERVWAEYVPRLE
                     DVQRMVGHPEPPPAIADPGGLGAQLHYLDVLWNAVAQSLEDAIRSSSR"
     gene            complement(282..1358)
                     /locus_tag="VF516_29160"
     CDS             complement(282..1358)
                     /locus_tag="VF516_29160"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEX8111850.1"
                     /translation="MRALLPLTLITVLDPGRARADPPRPRLRDWGVAIGALPPGPLDA
                     ITDVPGVRVGQVTVARGAAINTGVTAILPHDRNLFRDKVRAAIVVGNAFGKLTGALQV
                     NELGELESPIVLTCTLCVGKAWDATAGYLLGLPGNEDVRSVNPVIGETNDGLLNDIRS
                     RPITEADVLRAIRDARPGPVDEGSVGAGRGTTALGWKGGIGTASRRVDNGATVGVLVQ
                     TNFGGTLTIAGAPIGRALGTGITARPSGGSIMIVVATDAALDHRQLDRLARRALIGLG
                     RAGSAMDHGSGDFVIAFSISHQPPVADDTLNPLFQAVADATEEAILNSLFRAQTVHGA
                     RGTAEALPLDRVRRLLQDHRVLPP"
     gene            complement(1473..1853)
                     /locus_tag="VF516_29165"
     CDS             complement(1473..1853)
                     /locus_tag="VF516_29165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111851.1"
                     /translation="MRATVSDQGSRDVSNLRSSRILAVLLSLPMCVVTACAADTAPEA
                     TTTQPILSGCHILRPYGWDNGFTVCSEPEYTYGSIDLGPGESATFYSGDYGYPYGTGS
                     VTVVCHSNGDGGWDETNRTCFAGG"
     gene            complement(2625..>5520)
                     /locus_tag="VF516_29170"
     CDS             complement(2625..>5520)
                     /locus_tag="VF516_29170"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8111852.1"
                     /translation="PEPAPTAAPAPAAAPAAALSAQTTPDLTDEELAKLSEQEAKTEV
                     ITVTGSLISRKEVDSPSPVSVVDKQKLNAAGVTNVGDILQKIPAQGNAINAQNNNGGN
                     GSVRINLRSLGAARTLTLMNGRRVVPSGNGANSSVDFGTVPLAMIERIEVLKDGASAI
                     YGSDAIAGVVNVITRSNMNGTEANIYTSTSGKRDGTNYDLSFVTGHSSNRGNITFSGG
                     YQQQKPVMAGDRAFSAVTYAYDFSCTPEDEVAGKCSKLTLTGSSSSPNGRINAKNLTV
                     PGCNPDPSFPYCTADVNGGFRNFMKPSATSFGDNYNFQPLNYLFTPSTRVNLFSNGHY
                     DITKNTHVFFEGQFNLRKSQQQLAETPVQLSLFRTPISKDSIYNPFGKDVTDYNRRLV
                     EFGPRVFTQEVNTNRMVFGLDGTIPEDTAPVIANWKWEASYNYGRTDSTNQNTGNLIK
                     SHFANSIGPSFIDANGPQCGTRGNPIPGCVPMDLLHPGHIDQASINYMTFTGTQSGAN
                     EQHTALLTASGKIVDLPNHGDISLAVGGDYRFERGSSIPDPLTSTGDTTGNASQPTIG
                     SYNTFEGFSELSIVPYSGGDVVKWAEVNAAGRAYRYNTFGSGATGKVSGLLRTMGGVA
                     LRGTFGTAFRAPSVAELFAGQGDTFPAIEDPCDTKPPSRKTQMPLDPNSIAAMKCKDQ
                     GVPADALFGTSQQRSLTGGNPTLKPEHGNVTTAGVVYEPLRSLDFTLDYWRINIDKAI
                     TTLQPSTILAQCYQGGIQSFCDQVQRDPQTHVISHLVNIVQNVGGVATSGLDFSAAYQ
                     YMNAAGTFRHAIEGTYLFKYNVDTGTIDPQDPAKGNKIVHGRGYYDLGVNPDLKFNIF
                     TLWNHPSGIGAGFNLRFIDSFKECNQNNCNVVPTTPMVRHDVAKYATGDLYLAYVLKS
                     SQGATQITAGMNNVTNSTPAVIYNGGGLNSDESAYDFMGRQFYLRLSQLF"
BASE COUNT          818 a         1752 c         1984 g          966 t
ORIGIN      
        1 ccgagcccga cgcgagcgcg gaacacgcca cccgtggccc gcgcgcggtg cgcgacgtcg
       61 gcccggccga gctcacgccg gcgttcgaca ccgcggtgga gcgggtgtgg gccgagtacg
      121 tgccgcggct ggaggacgtg cagcggatgg tcggccatcc cgagccaccc ccggcgatcg
      181 ccgatcccgg cgggctgggc gcgcagctcc actacctcga tgtcctgtgg aacgcggtcg
      241 cgcagtcgct cgaggatgcg atcagaagct cgtcgagata gtcacggcgg caggacgcgg
      301 tgatcctgga gcagccggcg gacccggtcg agcggcagcg cctcggcggt gccgcgcgcg
      361 ccgtgcacgg tctgggcgcg aaacagcgag ttgaggatcg cctcctcggt ggcgtcggcg
      421 accgcctgga acagcgggtt gagcgtgtcg tcggcgaccg gcggctggtg cgagatcgag
      481 aacgcgatca cgaagtctcc cgagccgtgg tccatcgcgc tgcccgcgcg gcccaggccg
      541 atcagcgcgc ggcgggcgag ccgatcgagc tggcggtggt cgagcgcggc gtcggtcgcg
      601 acgacgatca tgatgctgcc gccggatggc cgggcggtga tgccggtgcc gagcgcgcgg
      661 ccgatcggcg cgccggcgat cgtcagcgtg ccgccgaagt tggtctggac cagcacgccg
      721 accgtggcgc cattgtcgac ccggcggctc gcggtgccga tgccgccctt ccagcccagc
      781 gcggtggtgc cgcggccggc gccgacgctg ccctcgtcga ccggccccgg gcgggcgtcg
      841 cggatcgcgc gcagcacgtc ggcctcggtg atcggccggc tgcggatgtc gttcaggagg
      901 ccgtcgttgg tctcgccgat caccgggttg accgagcgga cgtcctcgtt gccgggcagg
      961 ccgagcaggt agcccgcggt cgcgtcccac gccttgccga cgcacagcgt gcaggtcagg
     1021 acgatcggcg actcgagctc gccgagctcg ttgacctgca gcgcaccggt cagcttgccg
     1081 aacgcgttgc cgacgacgat cgccgcgcgc accttgtcgc ggaacaggtt gcgatcgtgg
     1141 ggcaggatcg cggtgacgcc ggtgttgatc gcggcgccgc gggcgacggt gacctggccg
     1201 acccgcacgc cgggcacgtc ggtgatcgcg tcgagcggac cgggcggcag cgcgccgatc
     1261 gcgacgcccc agtcgcgcag ccggggccgc ggcggatcgg cgcgggcgcg gcccgggtcg
     1321 aggacggtga tcagggtcag cgggagcaga gctcgcatgg cgcgatcgta gcgcgccggt
     1381 cagagcgcta cgacagccgg atgtgcgcga accggcccgc gatcgccgcc cagcgctcgc
     1441 gtggcgagcc gtcggcgcgc ctggacggaa tgtcacccgc cggcaaagca ggtcctgttt
     1501 gtctcgtccc agcccccgtc gccattgctg tgacatacca cggtcacgct gccggtgccg
     1561 tatgggtacc catagtcacc actgtagaag gtggcgctct cccctggtcc gaggtcgatc
     1621 gagccgtagg tatattctgg ctcggagcag accgtaaagc cgttatccca gccatacggc
     1681 cgaagaatgt ggcagcctga gaggatcggc tgggtcgtgg tggcctcggg tgcggtgtcc
     1741 gcggcgcacg cagtcaccac gcacatcggc aacgacagca gcaccgcgag aatccgcgac
     1801 gagcggaggt tcgagacatc acgtgatcct tggtccgaga ctgttgcccg catgttccat
     1861 ctccttgcac tggattgcac gggtctcgcg gcgacatggc cgcgagaccc gtgccatggg
     1921 cagagcaagc gatgggccac gcgcgcagac tcgacaactt gtcgacattg cgcgagatct
     1981 agagttctcc gtccgccgca aacgaccgtg acctgttcgg gtgcgcgttc cgatgtgttc
     2041 cgccccgagc agacgccccg gaacagctcg ctgttccggg tgttccgatc tcacgcgcgg
     2101 ccgtcggcgc ggccgccttt gcggggcggc ggccggcgcg gcgatcagaa cagctgcgac
     2161 agcaccatgt acgcgaccgg cccatgtagt cgtacgtgct ggagtccgac gtgccggcga
     2221 agccaccgag atccatcgac ccttgtaggt gcggctcaac aaattgccga gctgctcgac
     2281 gcggcctcgc agtggaaggt cgtcgcggcg tcgttcaccg aggtgcgctc gctggaacag
     2341 ctccgccagt ccctcggtcc gatcccgtcg ccgacggctc cggggcttcg ccatggcgaa
     2401 cgtgaacatc gcgaagggga ccgcggcgcc gtaccggatg agcgcattcc tcatcgccgt
     2461 tcccggtccg aacgggacct ggtcggtggt cgccgtgaac tacggggcgt tgtagacgct
     2521 cacgggactc acggtcgcga ctgcggcttc gcgatccggg cagaaccgca cgcccgcgcc
     2581 gctgtgacga cctcacgcag cggggcgtgc ggcagatcga gcgatcagaa cagctgagac
     2641 aggcgcaggt agaactgccg gcccatgaag tcgtaggccg attcgtcgga gttcaagccg
     2701 ccaccgttgt agatgaccgc cggtgtcgag ttggtgacgt tgttcatgcc cgcggtgatc
     2761 tgcgtcgcgc cctggctgct cttgagcacg taggccaggt agaggtcgcc ggtagcgtat
     2821 ttcgcgacat cgtgccgtac catcggcgtc gtcggcacga cgttgcagtt gttctggttg
     2881 cactccttga atgagtcgat gaaccgcaag ttgaagccgg cgccgatgcc cgatggatgg
     2941 ttccacagcg tgaagatatt gaacttcaga tccgggttga cgccgaggtc gtagtagccg
     3001 cggccgtgga cgatcttgtt gcccttcgcc ggatcctgcg ggtcgatcgt accggtgtcg
     3061 acgttgtact tgaacagata cgtgccctcg atggcgtgcc ggaacgtacc cgcggcgttc
     3121 atgtactgat atgccgccga gaagtcgagg cccgaggtcg ccacgccacc gacgttctgc
     3181 acgatgttga ccaggtggct gatcacgtgg gtctgcggat cgcgctggac ctggtcgcag
     3241 aagctctgga tgccgccctg gtagcactgc gcgaggatcg tcgagggctg cagcgtggtg
     3301 atcgctttgt cgatgttgat tcgccagtag tcgagcgtga agtcgagact gcgcagcggc
     3361 tcgtacacga cgccggcggt cgtcacgttg ccgtgctcgg gcttgagggt gggattgccg
     3421 ccggtcagcg agcgctgctg cgaggtgcca aacagcgcgt cggccgggac gccctgatct
     3481 ttgcacttca tcgcggcgat gctgttcgga tcgaggggca tctgcgtctt ccggctcggc
     3541 ggcttggtgt cgcacggatc ctcgatcgcg gggaacgtgt cgccctgacc ggcgaacagc
     3601 tcggcgacgc tgggggcgcg gaacgccgtg ccgaacgtgc cgcgcaacgc cactccgccc
     3661 atggtgcgga ggaggccgct gaccttgccg gtcgcgcccg agccgaacgt gttgtagcga
     3721 tacgcgcggc ccgcggcatt gacctcggcc cacttcacga catcgccgcc cgaatacgga
     3781 acgatcgaca gctcgctgaa gccctcgaac gtgttgtacg agccgatcgt cggctgcgac
     3841 gcgttgccgg tggtgtcgcc ggtcgaggtg agcggatcgg ggatcgacga gccgcgctcg
     3901 aaccggtagt cgccgccgac cgcgagcgag atgtcaccgt ggttgggcag gtcgacgatc
     3961 ttgccactcg cggtgagcag cgcggtgtgc tgctcgttgg cgccggactg cgtgccggtg
     4021 aacgtcatgt agttgatcga cgcctggtcg atgtggcccg gatggagcag atccatcggg
     4081 acgcagccgg ggatcgggtt gcctcgcgtg ccgcactgcg gcccgttggc atcgatgaag
     4141 ctcggaccga tcgagttcgc gaagtggctc ttgatcaggt tgcccgtgtt ctgattggtc
     4201 gagtcggtcc ggccgtagtt gtacgacgct tcccacttcc agttggcgat gaccggcgcg
     4261 gtgtcctcgg gtatcgtgcc gtcgaggccg aacaccatgc ggttggtgtt gacctcctgg
     4321 gtgaagacgc gcgggccgaa ctcgacgagg cgccggttgt agtcggtgac gtccttgccg
     4381 aacgggttgt agatgctgtc cttcgagatc ggcgtgcgga acagactgag ctgcaccggg
     4441 gtctcggcga gctgctgctg cgacttgcgc aggttgaact ggccctcgaa gaacacgtgc
     4501 gtgttcttcg tgatgtcgta gtggccgttc gagaacaggt tgacgcgcgt cgacggtgtg
     4561 aacaggtagt tcagcggctg gaagttgtag ttgtcgccga agctcgtcgc cgatggcttc
     4621 atgaagttgc ggaagccgcc gttgacgtcc gccgtgcagt acgggaagct cgggtccggg
     4681 ttgcagcccg ggacggtcag gttcttggcg ttgatgcggc cgttcggact cgacgacgag
     4741 ccggtcagcg tcagcttgga gcacttgccc gcgacctcgt cctccggcgt gcaggagaag
     4801 tcgtacgcgt aggtcaccgc gctaaaggcg cgatcgccgg ccatgaccgg cttctgttgc
     4861 tgatagccgc ccgagaacgt gatgttgccg cgattcgacg agtggccggt cacgaagctc
     4921 aggtcgtagt tcgtgccgtc gcgcttcccc gacgtcgagg tgtatatgtt ggcctcggta
     4981 ccattcatgt tcgaccgcgt gatcacgttg accacgccgg cgatcgcgtc cgagccgtag
     5041 atcgccgagg cgccatcctt gaggacctcg atgcgctcga tcatcgcgag cggcaccgtg
     5101 ccgaagtcga ccgagctgtt ggcgccgttg ccgctcggca cgacgcggcg gccattcatc
     5161 agcgtcagcg tgcgggcggc gccgaggctg cgcaggttga tgcggaccga gccattgcca
     5221 ccgttattgt tctgggcatt gatcgcattg ccctgggccg ggatcttctg aaggatatcg
     5281 ccgacgttgg tgacaccggc ggcgttgagc ttctgcttgt cgaccacgga caccggcgac
     5341 ggcgaatcga cctccttgcg gctgatcagc gagcccgtga cggtgatgac ctcggtcttg
     5401 gcctcttgct cggacagctt ggcgagctcc tcgtccgtca ggtccggcgt cgtctgggcg
     5461 gacagcgccg ccgccggcgc tgcggcggga gcgggcgcgg ccgtgggtgc cggctcgggg
//
LOCUS       DASVOR010001551         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_308317,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001551 DASVOR010000000
VERSION     DASVOR010001551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308317"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1492
                     /locus_tag="VF516_29175"
     CDS             <1..1492
                     /locus_tag="VF516_29175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX8111853.1"
                     /translation="KWEYPWYAAWDLAFHTVPLMMVDPDFARDQLDLMLSPEYLHPTG
                     QLPAYEWNFSDVNPPVHAFATLMVHRAAIADGEDVDLQFLKEAFAKLLLNFTWWVNRK
                     DPSGHNVFQGGFLGLDNIGVFDRSAALPTGGQLEQADGTAWMALFSQNMLSLALLLAE
                     RDASYEDFVLQFLHRFFSIAAAVDPIGDRPDERWDQEDGFFYDVLRLPDGRGVRLAVR
                     SLVGLLPLCATTVFESGVLDKFPRLRAAVASHIERNRDLLVNVADPLKPGVHGRHMLS
                     LVNEDKLRRILGHMLDESRFLGPHGIRSISRWHLEHPYSFRVDGHEYEVQYEPAESTT
                     GMFGGNSNWRGPVWFPINLLIVRALLQHYAYYGDAFTVECPTGSGKQMTLFEVAIELS
                     NRLIRTFTRGADGRRPVYGGAELFQRDEHWRDLILFYEYFHGDNGAGLGASHQTGWTG
                     VVARMIQLVGSLDAGTILREGSRSLATPYLGKPRSAARQQAAGGLR"
     gene            1492..>2646
                     /locus_tag="VF516_29180"
     CDS             1492..>2646
                     /locus_tag="VF516_29180"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8111854.1"
                     /translation="MAWPDHPVVHEIFTWAWLTELGARRGRELTLADVPEEVWDDVAR
                     PGIDAVWLMGVWQRSPMGAQIARTNPALRAAHQAALPGFGDADVVGSAYCVRDYVVDR
                     RLGGEDGLARARAALGRRGVRLVLDLVPNHVAPDHRWAREHPEYFIQGTPADLEREPH
                     AFLPIGDRVLACGRDPYFPAWPEVLQLDASSAPLRAAMVELVGGLARRCDAVRCDMAM
                     LLLDDVFARTWGARARGGPHPDGGRGYWPTIIGATKAARPDFQFWAEAYWDLEPVLLE
                     QGFDACYDKRLYDRIVHGGAIDVAAVRAHLGADIRYQRRTIRFVENHDEPRAASLLGP
                     AAHRAALATVFTLPGIALLHEGEADGRRVRVPVTLGRRPAEEPDRELRMFV"
BASE COUNT          350 a          953 c          929 g          414 t
ORIGIN      
        1 caagtgggag tatccgtggt acgcggcgtg ggacctcgcg ttccacaccg tgccgctgat
       61 gatggtggat cccgacttcg cccgcgatca gctcgacctg atgctgtcgc cggagtacct
      121 gcacccgacc gggcagctcc cggcctacga gtggaacttc agcgacgtga acccgccggt
      181 gcacgcgttc gcgacgctga tggtgcaccg cgcggcgatc gcggacggcg aggacgtcga
      241 tctgcagttc ctgaaggagg cgttcgccaa gctgctgctg aacttcacct ggtgggtcaa
      301 ccgcaaggat ccgagcggcc acaacgtgtt ccagggcggg ttcctcgggc tcgacaacat
      361 cggggtgttc gaccgcagcg cggcgctgcc gaccggcggc cagctcgagc aggccgacgg
      421 cacggcctgg atggcgctgt tcagccagaa catgctgagc ctcgcgctgc tgctcgccga
      481 gcgcgacgcg tcctacgagg acttcgtgct ccagttcctc catcgcttct tctcgatcgc
      541 cgccgcggtc gatccgatcg gcgatcggcc cgacgagagg tgggatcaag aggacggctt
      601 cttctacgac gtgctgcgcc tgccggacgg ccgcggggtc cggctcgcgg tgcgctcgct
      661 ggtcgggctc ctgccgctgt gtgcgaccac ggtgttcgag tccggggtgc tcgacaagtt
      721 cccccggctc cgcgccgcgg tggcgagcca catcgagcgc aaccgcgacc tgctggtcaa
      781 cgtcgccgat ccgctcaagc ccggtgtcca cggccggcac atgctgtcgc tggtcaacga
      841 ggacaagctt cgccggatcc tcgggcacat gctcgacgag tcccggttcc tcggcccgca
      901 cggcatccgg tcgatctcgc gctggcacct cgagcacccg tactcgttca gggtcgacgg
      961 ccacgagtac gaggtccagt acgagcccgc cgagtcgacg accggcatgt tcggcggcaa
     1021 ctccaactgg cgcggcccgg tgtggtttcc gatcaacctg ttgatcgtcc gggcgctgct
     1081 ccagcactat gcgtactacg gcgacgcgtt caccgtcgag tgtcccaccg ggtcgggcaa
     1141 gcagatgacg ctgttcgagg tcgcgatcga gctgtcgaac cggctgatcc gcacgttcac
     1201 gcgcggcgcg gacggccgcc ggccggtgta cgggggcgcc gagctgttcc agcgcgacga
     1261 gcactggcgc gacctgatcc tgttctacga gtacttccac ggcgacaacg gggccgggct
     1321 cggcgcgtcg caccagaccg gctggaccgg cgtcgtcgcg cggatgatcc agctggtcgg
     1381 ctccctcgat gccgggacca tcctgcgcga gggctcgcgt tcgctggcga cgccgtatct
     1441 cggcaagccg cgcagcgccg cgcgccagca agcggccgga gggctgcgct gatggcctgg
     1501 ccggatcacc ccgtcgtcca cgagatcttc acctgggcct ggctgaccga gctcggcgcg
     1561 cgtcgcggtc gcgagctcac gctggccgac gtccccgagg aggtgtggga cgacgtcgcc
     1621 cggccgggga tcgacgccgt gtggctgatg ggcgtgtggc agcgcagccc gatgggtgcg
     1681 cagatcgcgc gcacgaaccc ggcgctgcgc gcggcacatc aagccgccct gccggggttc
     1741 ggcgacgccg acgtggtggg atccgcctac tgcgtccgcg actacgtcgt cgaccgccgg
     1801 ctcggtggcg aggacggtct ggcgcgggcc cgcgcggcgc tcgggcggcg cggcgtccgc
     1861 ctggtgctcg atctcgtgcc caaccacgtg gcgcccgacc atcgctgggc ccgcgagcac
     1921 cccgagtact tcatccaggg taccccggcc gatctcgagc gcgagcccca cgcattcctc
     1981 ccgatcgggg atcgggtgct ggcctgcggg cgcgatccgt actttccggc gtggccggag
     2041 gtgctgcagc tcgatgcgtc cagcgccccg ctgcgcgccg cgatggtcga gctggtcggc
     2101 ggcctggcgc gccggtgcga tgccgtgcgc tgcgacatgg ccatgctgtt gctcgacgac
     2161 gtgttcgcgc gcacctgggg cgctcgcgcg cgcggcgggc cgcatcccga cggcggccgc
     2221 ggctactggc cgacgatcat cggcgcgacc aaggcggcgc gccccgactt ccagttctgg
     2281 gccgaggcct actgggatct cgagccggtg ctgctcgagc agggcttcga cgcatgctac
     2341 gacaagcggc tctacgatcg catcgtgcac ggcggcgcga tcgacgtcgc tgccgtgcgc
     2401 gctcacctcg gcgcggacat ccgttaccag cgccgcacga tccgcttcgt ggagaaccac
     2461 gacgagcccc gcgcggcatc gctgctcggc cccgccgcgc accgcgccgc gctcgccacc
     2521 gtgttcacgc tccccggcat cgcgctgctc cacgagggcg aggccgacgg tcgccgggtc
     2581 cgggtccccg tcacgctggg ccgccgaccc gccgaggaac ccgatcgcga gctccggatg
     2641 ttcgtc
//
LOCUS       DASVOR010001552         4699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_512637,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001552 DASVOR010000000
VERSION     DASVOR010001552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4699
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_512637"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="VF516_29185"
     CDS             complement(<1..721)
                     /locus_tag="VF516_29185"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111855.1"
                     /translation="MAALLELDRVTAGYGASVVLDGVSLAVAEADALAVLGRNGVGKT
                     TLLVTVMGLTTLHRGTLRWQGRDLGAMATFQRTRAGIGWVPQERLVFPSLTVHEHLLA
                     VARPGRWTPPRIYELFPGLARRRGNLGDQLSGGEQQMLAIARALMTNPRLLLLDEPLE
                     GLAPIVAQELVRVIRALRADAGMAIVVVEQHARIALSLTEHAIVLDRGRIVHRSTSRG
                     LLDSPGLLHRLLIGDREAEDGA"
     gene            complement(724..1524)
                     /locus_tag="VF516_29190"
     CDS             complement(724..1524)
                     /locus_tag="VF516_29190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111856.1"
                     /translation="MTDGAGAAPRVPSPPVALRTEGLCKRWGVVYVNDRIDLTLPVGA
                     RHALIGPNGAGKTTLVNLLTGLYPPTAGRVLLGDTDVTRLAMHERVKRGLVRTFQINT
                     LFPGLTVLESVVLAICERTGAARRWARSVAQLDAPIAEAEALLDRLGLGSVAHTVTRN
                     LPYGKQRLLEIALALALQPRVLLLDEPASGIPAGDSTEVFEVIAALPGDVTVLFIEHD
                     MELVFRFAQRIAVLVDGRLLTEGTPAEIAADPRVRDVYLGETASEKLG"
     gene            complement(1521..2510)
                     /locus_tag="VF516_29195"
     CDS             complement(1521..2510)
                     /locus_tag="VF516_29195"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8111857.1"
                     /translation="MTGRGRATGGGLPSDRWRIWDTALWLVPVAAYFVFPDHLVLGSQ
                     VIIAALFALSLDVVLGYAGITSLGHAAFFGLGAYTAGLLAAHGWGEPLTGLVVAAVVA
                     AVAGYLTSFLVVRGSALAQLMITLGVDLLLREVANKATRWTGGSDGLSDMTVGKLLGV
                     FRFDLAGRTGYVYSLVVALGAFVLVRRVVSSPFGLSLRAIREGAARMPTLGAPVGRRL
                     VAAFAFGAGLAGIAGALLTQTTQAVALEVLGTERSASVLIMLVLGGTGRLYGAFIGAG
                     LFMLMQDFLARRDPMYWQLWIGCLLVAVVLVGRGGILGIAEALRARWAGRSAP"
     gene            complement(2507..3370)
                     /locus_tag="VF516_29200"
     CDS             complement(2507..3370)
                     /locus_tag="VF516_29200"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8111858.1"
                     /translation="MAAFITVLFDGVAYGSLLFVISVGMSVTLGLMRFINLAHGAFAM
                     FGGYTACVLADRCGLSIFASLPVVLVGFAAVGALVERMVYRWVSRTRPLDQVLLTIGI
                     AFIATAAAYKIWGPYPRRLAVPEALTGQLHVLGVDLGVYRLFLIAMVVVITAALHVLI
                     ERTRFGAQLRAAVDRPEAGAGLGIPVNRVFTASFAIGSGLAAVGGVLGVGMLGLDFNF
                     TFRYMVYFLLVVVVGGEGSILGSALAAVALGVFDVVGKYYFPSVGAFLIYALMVVLLL
                     VFPHGLIRRGR"
     gene            complement(3417..>4699)
                     /locus_tag="VF516_29205"
     CDS             complement(3417..>4699)
                     /locus_tag="VF516_29205"
                     /inference="COORDINATES: protein motif:HMM:NF024825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8111859.1"
                     /translation="SMSHPAFLRIALAGLMVVPVVVPVVGGAACNKKTDDAKPAAGSG
                     SAPAATAPQAKTTIKIGMVASFSGPFADISKQLMGGVKTYMKQHGDTVAGKKIELVTR
                     DTTGPAPEVAKRLAQELVVQDKVDFLTGFGLTPEALGAAPVATEGKTPMVIMNAATSV
                     ITTKSPYIVRFSLTLPQVCQPLGDWAAKNGIKKVVTMVSDYGPGHDAEAAFTKAFTAG
                     GGQVIDAIQTPVQNPDFSSFIQRVKDKQPDAVFVFVPAGQQSIAAMKAYDERGLGKAG
                     IKLIATGDVLDDHNLATIGDAALGAISSYHYSVAHDSPENKAFLDAYRDANGADAGLP
                     NFMSVAAYDSMAAIYDVIQKLDGKIDPDKAMDILKHEAFTSPRGPISIDPETRDIVQT
                     VYLRRVDKVNGELRSIELQPLVAAMKDPGKDPGK"
BASE COUNT          742 a         1724 c         1625 g          608 t
ORIGIN      
        1 cggcgccatc ctctgcctcg cggtccccga tcagcaggcg atgcaggagg cccgggctgt
       61 cgagcaggcc gcggctcgtg ctccggtgca cgatccggcc gcggtcgagc acgatcgcat
      121 gctcggtcag agacagcgcg atccgcgcgt gctgctcgac cacgacgatc gccatgccgg
      181 cgtccgcgcg cagcgcgcgg atcacgcgga ccagctcctg ggcgacgatc ggcgccaggc
      241 cctcgagcgg ctcgtcgagc agcagcagcc gcgggttggt catcagcgcg cgcgcgatcg
      301 ccagcatctg ctgctcgccg cctgacagct ggtcgccgag gttgccgcgc cggcgcgcca
      361 ggccggggaa cagctcgtag atccgcggcg gggtccatcg cccgggccgc gcgaccgcga
      421 gcaggtgctc gtgcacggtg agcgacggga acaccaggcg ctcctgcggg acccagccga
      481 tgccggcgcg ggtgcgctgg aaggtcgcca tcgcgccgag gtcgcggccc tgccagcgca
      541 gcgtgccgcg atgcagggtg gtcagcccca tcaccgtgac cagcagcgtg gtcttgccga
      601 ccccgttgcg gccgagcacg gcgagcgcgt cggcctcggc gacggccagc gacacgccgt
      661 cgagcacgac cgacgcgccg tagccggcgg tgacccgatc gagctcgagc agcgcggcca
      721 tcgctagccc agcttctccg atgcggtctc gccgaggtag acgtcgcgga cccgcgggtc
      781 ggcggcgatc tcggccggcg taccctcggt gagcaggcgg ccgtcgacca gcaccgcgat
      841 gcgctgggcg aaccggaaca ccagctccat gtcgtgctcg atgaacagca cggtgacgtc
      901 gccgggcagc gcggcgatca cctcgaacac ctcggtgctg tcacccgccg ggatccccga
      961 cgccggctcg tcgagcagca gcaccctggg ctggagcgcc agcgcgagcg cgatctcgag
     1021 caggcgctgc ttgccgtacg gcaggttgcg ggtgacggtg tgcgccaccg agcctagccc
     1081 gagccggtcg agcagcgcct cggcctcggc gatcggcgcg tcgagctgcg ccacgctgcg
     1141 cgcccagcgc cgcgcggccc cggtgcgctc gcagatcgcg agcacgaccg actccagcac
     1201 ggtgagcccg gggaacagcg tgttgatctg gaacgtgcgc accaggccgc gcttgacccg
     1261 ctcgtgcatc gccagccggg tgacgtcggt gtcgcccagc agcacccgcc cggcggtcgg
     1321 cgggtacagg ccggtcagca ggttgaccag cgtggtcttg ccggcgccgt tggggccgat
     1381 cagcgcgtgg cgcgccccga ccggcagcgt caggtcgatg cgatcgttga cgtagaccac
     1441 gccccagcgc ttgcagagcc cctcggtgcg gagcgcgacg ggcggcgagg gaacccgcgg
     1501 cgcggctccg gcaccgtcgg tcacggcgcg ctccgccccg cccatcgcgc gcgcagcgcc
     1561 tcggcgatac ccaggatgcc gccgcgcccg accagcacca cggcgaccag caggcagccg
     1621 atccacagct gccagtacat cggatcgcgg cgcgcgagga agtcctgcat cagcatgaac
     1681 agcccggcgc cgatgaacgc gccgtacagc cggccggtgc cgccgagcac gagcatgatc
     1741 agcaccgacg ccgagcgctc ggtcccgagc acctccagcg cgacggcctg cgtggtctgg
     1801 gtcagcagcg cgccggcgat cccggccagc cccgcgccga acgcgaacgc ggcgaccagc
     1861 cgccggccga ccggtgcgcc gagcgtgggc atccgggccg cgccctcgcg gatcgcgcgc
     1921 agcgacaggc cgaacggcga gctcaccacg cggcggacca ggacgaacgc accgagcgcg
     1981 acgaccaggc tgtacacgta gccggtgcgg cctgcgaggt cgaaccggaa cacgccgagc
     2041 agcttgccga ccgtcatgtc cgacagcccg tcactgccgc cggtccaccg cgtcgccttg
     2101 ttggcgacct cccgcaggag caagtcgacg ccgagcgtga tcatcagctg ggcgagcgcg
     2161 ctgcctcgca cgaccaggaa gctggtcagg tagcccgcca ccgccgcgac caccgccgcg
     2221 accacaaggc cagtcagcgg ctcgccccag ccgtgcgcgg cgagcagccc ggcggtgtag
     2281 gcgccgagcc cgaagaacgc ggcgtggccg agcgaggtga tgccggcata gccgagcacg
     2341 acgtcgagcg acagcgcgaa cagcgcggcg atgatcacct ggctgccgag cacgaggtga
     2401 tcagggaaca cgaagtaggc cgcgaccggc accagccaca gcgcggtgtc ccagatccgc
     2461 cagcgatcgc tgggcagccc accgccggtc gccctgcccc gcccggtcat cgccccctcc
     2521 ggatcaggcc gtgcgggaac accagcagca ggaccaccat cagcgcatag atcaggaacg
     2581 cgccgacgct cgggaagtag tacttgccga ccacgtcgaa cacgcccagc gcaacggcgg
     2641 ccagcgcgct gccgaggatc gagccctcac cgccgaccac cacgacgagc aagaagtaca
     2701 ccatgtagcg gaaggtgaag ttgaagtcga ggccgagcat cccgacgccg agcacgccgc
     2761 cgaccgcggc gagcccgctg ccgatcgcga agctcgcggt gaacacgcgg ttgaccggaa
     2821 tgccgagccc cgcgccggcc tccgggcggt cgaccgccgc gcgcagctgg gcgccgaacc
     2881 gggtgcgctc gatcagcaca tgcagcgccg cggtgatcac gacgaccatc gcgatcagga
     2941 acagccggta gaccccgaga tcgacgccga gcacatggag ctggccggtc agcgcctccg
     3001 gcaccgcgag gcggcgtggg tacggccccc agatcttgta cgccgcggcg gtcgcgatga
     3061 acgcaatccc gatcgtgagc agcacctggt cgagcggccg ggtccgcgac acccagcgat
     3121 agaccatccg ctccaccagc gcgccgaccg ccgcgaaccc gaccaggacc accggcagcg
     3181 acgcgaagat cgacaggccg cagcgatcgg cgagcacgca cgcggtgtag ccgccgaaca
     3241 tcgcgaacgc gccgtgcgcc aggttgatga accgcatcag gcccagcgtc accgacatcc
     3301 cgacgctgat gacgaacagc aggctgccgt acgccacccc atcgaacagc acggtgatga
     3361 acgcggccat ggctcagcgg ccccgagcct agcgtggctc ggctcgctcc gcgtgactac
     3421 ttgccgggat ccttgccggg gtccttcatc gccgcgacca gcggctggag ctcgatgctg
     3481 cgcaactcac cgttcacctt gtcgaccctc cgcaggtaca cggtctgcac gatgtcgcgg
     3541 gtctcggggt cgatcgagat cgggccgcgc gggctggtga acgcctcgtg cttcaggatg
     3601 tccatcgcct tgtcggggtc gatcttgccg tcgagcttct ggatgacgtc gtagatcgcc
     3661 gccatcgagt cgtaggccgc gaccgacatg aagttcggca ggcccgcgtc ggccccgttg
     3721 gcgtcgcggt acgcgtcgag gaacgccttg ttctccggcg agtcgtgcgc caccgagtag
     3781 tggtagctgg agatcgcccc cagcgcggcg tcaccgatcg tcgccaggtt gtggtcgtcg
     3841 agcacgtcgc cggtggcgat cagcttgatc ccggccttgc ccagaccgcg ctcgtcgtag
     3901 gccttcatcg ccgcgatgct ctgctggccg gccggcacga acacgaacac cgcgtcgggc
     3961 tgcttgtcct tgacccgctg gatgaacgac gagaagtcgg ggttctggac cggcgtctgg
     4021 atcgcgtcga tcacctggcc gccgcccgcc gtgaacgcct tggtgaacgc cgcctcggcg
     4081 tcgtgcccgg gtccgtaatc ggagaccatc gtcacgacct tcttgatccc gttcttggcc
     4141 gcccagtcgc cgagcggctg gcagacctgg ggcagggtca gcgagaaccg cacgatgtac
     4201 ggcgacttgg tcgtgatgac cgaggtcgcc gcgttcatga tcaccatcgg ggtcttgccc
     4261 tcggtggcca ccggcgccgc gcccagcgcc tcgggggtca ggccgaagcc ggtcaggaag
     4321 tcgaccttgt cctgcaccac cagctcctgg gccaggcgct tggcgacctc gggcgccggc
     4381 ccggtggtgt cgcgggtcac aagctcgatc ttcttgcccg ccaccgtatc gccgtgctgc
     4441 ttcatgtacg tcttgacccc gcccatcagc tgcttgctga tgtcggcgaa cgggccggag
     4501 aacgaggcga ccatgccgat cttgatcgtc gtcttggcct gcggggccgt ggccgccggc
     4561 gcgctgccgg accctgccgc cggcttggcg tcgtcggtct tcttgttgca tgccgccccg
     4621 ccgaccaccg ggacaaccac cgggacgacc atgagacccg ccagcgcgat gcggaggaat
     4681 gccggatgag acatcgatg
//
LOCUS       DASVOR010001553         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_424375,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001553 DASVOR010000000
VERSION     DASVOR010001553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424375"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..648)
                     /locus_tag="VF516_29210"
     CDS             complement(<1..648)
                     /locus_tag="VF516_29210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111860.1"
                     /translation="MAYRDDAGDACEAPTADGMLRAELSGGDVRLALTARSLHVTDRF
                     ATVIEHHKKRTAKDRRSSIAIAGRLLIARDALREGLGVWIEVEPGTPRAGFRRIFGVE
                     PRSLLEPGGLGALAALDRLAQGVRQALAHLAPDVRRATEIGSPASGGLDKVLAVDHGE
                     RVVVYARGLFRDRARFAMAIHDDGRIAVRGGGAREVAVRSRHGVTVVGDYIRFADP"
     gene            801..>1592
                     /locus_tag="VF516_29215"
     CDS             801..>1592
                     /locus_tag="VF516_29215"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEX8111861.1"
                     /translation="MLLLIALGVAFFAGMGALTSLRVLREYERAIVFRLGRARQKALG
                     PGVVLMLPFGVDRATVVDTRTKVIQIPTQEVITRDNISIGVDAVVYIEISSPMDAILR
                     VEQYMPATLQLAATTLRSIIGRMELDEVLAKRAEINTEVQEILESRVEQWGVNITAVE
                     IKDISLPAEMKRAMARQAEAERERRAKIIMSEGELQAAQRLAEAARLMAGDPAALQLR
                     LYQTMVEVSAQPNMTIVLPVPIELLAGGGSAGAAGAAATQAAMAAA"
BASE COUNT          221 a          590 c          575 g          206 t
ORIGIN      
        1 cgggtcggcg aaccggatgt agtcgccgac caccgtgacg ccgtggcgcg atcgcaccgc
       61 aacctcgcgg gcccccccgc cgcgcacggc gatccggccg tcgtcgtgga tcgccatcgc
      121 gaaccgcgcg cgatcccgga acagcccgcg ggcgtacacc acgacgcgct cgccgtgatc
      181 gaccgcgagc accttgtcga gcccgcccga cgccggcgag ccgatctcgg tcgcgcgccg
      241 gacgtcgggc gcgaggtgcg cgagcgcctg ccgcacaccc tgcgccaggc ggtccagcgc
      301 ggccagcgcg cccagcccgc cgggctcgag caggctccgc ggctcgaccc cgaagatccg
      361 gcggaaccct gcgcgcggcg tgccgggctc gacctcgatc cacacgccga ggccctcgcg
      421 cagcgcgtcg cgcgcgatca gcagccgccc ggcgatcgcg atcgagctcc gccggtcctt
      481 cgcggtacgt ttcttgtggt gctcgatcac cgtcgcgaac cggtcggtga cgtggagcga
      541 tcgggcggtc agcgcgagcc ggacatcgcc tccggacagc tcggcgcgca gcatgccgtc
      601 ggcggtcggt gcctcgcacg catccccggc gtcgtcgcga tacgccacgc tccgagcgta
      661 accgagccgc gggcgcgcgc cagggccggt gccagggctg tgccgggccg gtgccgggcc
      721 ggtgccgggc cggtgccggc ttggtgcctg ctggtgatca atccgccctg ggcaaccgca
      781 acctgggcta tacctcccgc atgctcctgc tgatcgccct gggagtggcc ttcttcgcag
      841 gcatgggagc gctgaccagc ctgcgcgtgc tgcgcgagta cgagcgcgcg atcgtgttcc
      901 ggctcggccg ggcgcggcaa aaggcgctcg gacccggcgt cgtgctgatg ctgccgttcg
      961 gcgtcgaccg cgcgaccgtg gtcgacaccc gcaccaaggt catccagatc ccgacccagg
     1021 aggtgatcac gcgcgacaac atctcgatcg gggtcgacgc cgtcgtgtac atcgagatct
     1081 cgtcgccgat ggacgcgatc ctgcgggtcg agcagtacat gccggcgacg ctgcagctcg
     1141 ccgcgaccac gctgcgctcg atcatcggcc ggatggagct cgacgaggtg ctcgccaagc
     1201 gcgccgagat caacaccgag gtccaggaga tcctcgagtc gcgcgtcgag cagtggggcg
     1261 tcaacatcac cgcggtcgag atcaaggaca tctcgctgcc ggccgagatg aagcgcgcga
     1321 tggcgcgcca ggccgaggcc gagcgcgagc gccgcgccaa gatcatcatg tccgagggcg
     1381 agctccaggc cgcccagcgg ctcgccgagg ccgcgcggct gatggccggc gatccggcgg
     1441 cgctccagct ccggctctac cagaccatgg tcgaggtgtc ggcgcagccc aacatgacga
     1501 tcgtgctgcc ggtcccgatc gagctgctgg cgggcggggg ctcggcgggt gcggcgggcg
     1561 ccgcggcgac ccaggcggcc atggcagcgg cc
//
LOCUS       DASVOR010001554         1706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395141,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001554 DASVOR010000000
VERSION     DASVOR010001554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395141"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1210)
                     /locus_tag="VF516_29220"
     CDS             complement(<1..1210)
                     /locus_tag="VF516_29220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8111862.1"
                     /translation="MSFSQLGLLPALQRAVAGQGYVEPTPIQARAIPHVLAGRDLLGL
                     AQTGTGKTAAFALPILQRLSHILPAGRRRPIRCLVLTPTRELAAQIQDSFATYGEHLA
                     LTSFVIFGGVGMEAQKQALRAGMDILVATPGRLLDLAGQGLVELKALEVFVLDEADRM
                     LDMGFIHDVRKVIRLLPPRRQTLFFSATMPREAQELADQLLTRPETVAVVPPATTAER
                     VDQEVYFVEKADKRALLVDVLRDSAMRRVLVFSRTKHGANRIAEHLVKAGIGAEAIHG
                     NKSQNARERALAGFKSGKTRVLVATDIAARGIDIDDVTHVVNFDVPEVPETYVHRIGR
                     TARAGASGMALTFCEAAERADWRNIEKLTRQDIPIVEGHPYESRVPFAALPRAPAAPP
                     PAKGHGHGRRV"
     gene            complement(1282..>1706)
                     /locus_tag="VF516_29225"
     CDS             complement(1282..>1706)
                     /locus_tag="VF516_29225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111863.1"
                     /translation="TAVIVGDDGDRAAYARNLAANAAAPAPAKADRAAYYATGGSGPA
                     GSARASNDVVGGIVAGKMSKMNLHKFARTALPPELRGLDAAALQAEIERRAQLRKDTE
                     AEMSRLVKQRTEYLRANAKGGEGGFDAKVNATLDAQLK"
BASE COUNT          205 a          590 c          657 g          254 t
ORIGIN      
        1 ggacgcggcg gccgtggccg tggcccttgg ctggcggcgg tgcggccggc gcgcgcggca
       61 gcgcggcgaa tggcacgcgc gactcgtagg ggtggccctc gacgatcggg atgtcctggc
      121 gggtcagctt ctcgatgttg cgccagtcgg cgcgctcggc ggcctcgcag aacgtcagcg
      181 ccatgccgga cgcgccggcg cgcgcggtgc gcccgatccg gtggacgtag gtctcgggga
      241 cctcggggac gtcgaagttg acgacgtggg tgacgtcgtc gatgtcgatc ccgcgcgccg
      301 cgatgtcggt cgcgaccagc acgcgggtct tgcccgactt gaagccggcc agggcgcgct
      361 cgcgcgcgtt ctgcgacttg ttgccgtgga tcgcctcggc gccgatcccg gccttgacca
      421 ggtgctcggc gatccggttg gcgccgtgct tggtccgcga gaacaccagc acgcgccgca
      481 tcgcggagtc gcgcagcacg tcgaccagca gcgcgcgctt gtcggccttc tcgacgaagt
      541 agacctcctg atcgacgcgc tcggcggtgg tcgccggcgg cacgaccgcg acggtctcgg
      601 gccgggtcaa gagctggtcg gcgagctcct gggcctcgcg cggcatggtc gccgagaaga
      661 acagggtctg gcggcgcggc ggcaacagcc ggatcacctt gcggacgtcg tggatgaagc
      721 ccatgtcgag catgcggtcg gcctcgtcga gcacgaacac ctcgagcgcc ttgagctcga
      781 ccagaccctg gccggcgagg tcgagcaggc ggcccggggt cgcgaccagg atgtccatgc
      841 cggcgcgcag cgcctgcttc tgggcctcca tcccgacgcc gccgaagatc acgaagctgg
      901 tgagcgccag gtgctcgccg taggtcgcga agctgtcctg gatctgggcg gcgagctcac
      961 gggtcggggt caggaccagg cagcggatcg gccgccgccg gccggcggga aggatgtgcg
     1021 acaggcgctg caggatcggc agcgcgaacg ccgcggtctt gccggtgccg gtctgggcca
     1081 ggccgagcag gtcgcggccg gccaggacgt gcgggatggc gcgcgcctgg atcggcgtcg
     1141 gctcgacata gccttggccg gcgaccgcgc gctggagcgc ggggagcaac ccgagctggg
     1201 aaaacgacat cgcggcgaca tagcacgtct ggccggcgcg cacgccccgg ccggcgccgc
     1261 ttcacacggg ctgcacgccg ttcacttgag ctgtgcgtcg agcgtggcgt tgaccttggc
     1321 gtcgaacccg ccctcgccgc ccttggcgtt cgcccggagg tactcggtgc gctgcttgac
     1381 cagcctggac atctcggcct cggtgtcctt gcgcagctgg gcgcggcgct cgatctcggc
     1441 ctggagcgcc gcggcgtcga ggccgcgcag ctcgggcggc agcgcggtcc tggcgaactt
     1501 gtggaggttc atcttgctca tcttgccggc gacgatgccg ccgaccacgt cgttgctcgc
     1561 cctcgcgctg cccgccggcc cgctgccgcc ggtcgcgtag tacgcggcgc ggtcggcctt
     1621 ggccggtgcc ggcgcggcgg cgttggccgc caggttgcgc gcgtacgccg cgcgatcgcc
     1681 gtcgtcgccg acgatcaccg cggtgg
//
LOCUS       DASVOR010001555         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_571757,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001555 DASVOR010000000
VERSION     DASVOR010001555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571757"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VF516_29230"
     CDS             complement(<1..813)
                     /locus_tag="VF516_29230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111864.1"
                     /translation="MARVLAAGFVALVAALVGLGALLGAGGQVRADGDTLAALLRRTD
                     AVAREVARVRGLPLKRPIPNEVVDRSELHARLLKMAAEDKTAEDSAAEGFALERWGMI
                     PPGTDYKALLVDVLTEQVAGYYDPETKKLTISRSAGDDPAWAEMVLAHEIDHGLQDQS
                     FDLHHFEDIVSSEGDAAAARHALVEGDGIAVMIEVMLARRRAKVDWANPEIATAIEKS
                     MSAPGVGSDGLDRAPLAIREAMLFPYRAGFGFIAALRRRQPWSAVDAAFARPP"
     gene            complement(873..>1984)
                     /locus_tag="VF516_29235"
     CDS             complement(873..>1984)
                     /locus_tag="VF516_29235"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEX8111865.1"
                     /translation="PPPYPPLAPLGDAVFARRHDRLRGLAREAGASVVFVTSGTTSFA
                     YLAGGRVERSERLIALVLPVAGPPFLIAPAFEVERMFRQTRIHDVRPWEEHESPYAVA
                     RDALDAAHAGTKILIEPHTEYQTALALGRAIPGATLVDGTAVFEQLRLHKDDGELARI
                     RRAIAVTQDVFDAAFAGLRPGVRDREVSAAIAAEFRRAGYEGYALVQFGALSALPHGH
                     PSGDALRERTVVLIDGGCQVDGYWSDITRTRFFGGAPPAEFTRVHRIVRDAQTAAIER
                     VRPGVAAEDLDRAARDVIEHAGFGAQFTHRLGHGLGMDGHEPAYLVRGNPQRLEPGFV
                     FTVEPGIYLPDQFGVRIEDDVACGPDGAIVLSRRD"
BASE COUNT          264 a          754 c          723 g          243 t
ORIGIN      
        1 cggcggccgg gcgaacgcgg cgtcgacggc gctccacggc tggcggcggc gcagcgccgc
       61 gatgaagccg aacccggcgc ggtaggggaa cagcatcgcc tcgcggatcg cgagcggcgc
      121 gcggtcgagg ccgtcgctgc cgacccccgg cgccgacatg ctcttctcga tcgcggtcgc
      181 gatctcgggg ttggcccagt cgaccttggc gcggcggcgc gcgagcatga cctcgatcat
      241 gaccgcgatg ccatcgccct cgaccagcgc gtggcgcgcg gcggccgcgt cgccctccga
      301 gctcacaatg tcctcgaagt ggtggagatc gaacgactgg tcctgcaggc cgtgatcgat
      361 ctcgtgggcg agcaccatct cggcccacgc cggatcgtcg cccgccgagc gcgagatcgt
      421 gagcttcttg gtctcgggat cgtagtagcc ggcgacctgc tcggtcagga cgtcgaccag
      481 cagcgccttg tagtcggtgc ccggcgggat catgccccag cgctcgagcg cgaagccctc
      541 cgcggcgctg tcctcggcgg tcttgtcctc ggcggccatc ttgagcagcc gggcatggag
      601 ctcgctgcga tcgaccacct cgttggggat cggccgcttg agcggcaggc cgcgcacccg
      661 ggccacttcg cgggcgaccg cgtcggtgcg ccgcagcagc gccgcgagcg tgtcgccgtc
      721 ggcgcggacc tgcccacccg ccccgagcag cgcgcccaga ccgaccagcg ctgcgaccag
      781 cgcgacgaac ccggccgcga gcacacgagc catggccgga tcctacaccg ccgccgcggc
      841 ggcgccaggc gcggcgatcc gcggcgctgc cgtcagtcgc ggcgcgacag cacgatcgcc
      901 ccgtcgggcc cgcacgccac gtcgtcctcg atgcggacgc cgaactggtc gggcagatag
      961 atcccgggct cgaccgtgaa cacgaagccg ggctccagcc gctgggggtt gccgcgcacc
     1021 aggtacgccg gctcgtggcc gtccatgccc aggccgtgac cgaggcggtg ggtgaactgg
     1081 gcgccgaagc ccgcgtgctc gatgacgtcg cgcgccgcgc gatcgaggtc ctcggccgcc
     1141 actcccgggc gcacgcgctc gatcgccgcg gtctgggcgt cgcgcacgat ccggtggacg
     1201 cgggtgaact cggccggcgg cgcgccgccg aagaaccgcg tgcgggtgat gtccgaccag
     1261 tagccgtcga cctgacagcc gccgtcgatc aggaccaccg tccgctcgcg cagcgcgtcg
     1321 ccgctgggat ggccatgcgg cagcgccgac agcgcgccga actggaccag cgcgtagccc
     1381 tcgtaccccg cgcgccggaa ctcggccgcg atcgccgccg acacctcgcg atcgcgcacc
     1441 ccgggccgca ggccggcgaa cgcggcgtcg aacacgtcct gggtcaccgc gatcgcgcgc
     1501 cggatccgcg cgagctcgcc gtcgtccttg tgcagacgca gctgctcgaa caccgcggtg
     1561 ccgtcgacca gcgtcgcgcc ggggatcgcc cggccgagcg cgagcgcggt ctggtactcg
     1621 gtgtgcggct cgatcaggat cttggtgccg gcgtgcgcgg cgtcgagcgc atcgcgcgcc
     1681 acggcgtacg ggctctcgtg ctcctcccac ggacgcacgt cgtggatccg ggtctggcgg
     1741 aacatccgct cgacctcgaa cgccggcgcg atcaggaacg gcgggccggc gaccggcagc
     1801 accagcgcga tcagccgctc gctgcgctcg acccggccgc ccgccaggta cgcgaacgac
     1861 gtcgtgccgc tggtgacgaa cacgacgctc gccccggcct cgcgcgcgag cccgcgcagc
     1921 cgatcatggc gccgggcgaa cacggcgtcg ccgagcggcg ccagcggcgg gtacggaggc
     1981 ggcg
//
LOCUS       DASVOR010001556         2173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_454086,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001556 DASVOR010000000
VERSION     DASVOR010001556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2173
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454086"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="VF516_29240"
     CDS             complement(<1..318)
                     /locus_tag="VF516_29240"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HEX8111866.1"
                     /translation="MTRGDDDDDDQPIDEVGLAAARRRLDEIIAKHPELVGPTGPDNV
                     SDWLEVFIRLTGMTHVRTAPYYPQSNGKLERYHKTIKENAIRPGQPKTLEEARELVAR
                     WVEH"
     gene            complement(315..623)
                     /locus_tag="VF516_29245"
     CDS             complement(315..623)
                     /locus_tag="VF516_29245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111867.1"
                     /translation="MLTVIGQWEREAIGERTAAAMQHKASQGEYTGGAASYGYALAAD
                     GVTLVEVAIEQAVIAEARAHRATGLSLRKVAEELDRKGLRARNGRPLAPVQVARMVAG
                     "
     gene            complement(626..832)
                     /locus_tag="VF516_29250"
     CDS             complement(626..832)
                     /locus_tag="VF516_29250"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX8111868.1"
                     /translation="MARPALDLALAMLRTGKADALLVVKLHRLTRSVRDLCDLVDRYF
                     RDGKHALLSVGEQVDTRSAAGAWC"
     gene            complement(845..1003)
                     /locus_tag="VF516_29255"
     CDS             complement(845..1003)
                     /locus_tag="VF516_29255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX8111869.1"
                     /translation="MSDARTRTVAYLRVSTDKQAGRGVSLEAQRAKVEAYAALFDLNL
                     VAIEVDPG"
     gene            complement(1258..1917)
                     /locus_tag="VF516_29260"
     CDS             complement(1258..1917)
                     /locus_tag="VF516_29260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111870.1"
                     /translation="MVVAAATIALAGCEPPATIFRSRADLANTAPIERLLVIAHLDVD
                     GRVSRGFIGGLENRLAACGVASHVVVGPIAADPLDALHSAGGFQPSTALVVRSEGSDL
                     VKIEGNHNDKSGTLYFGFQLLDAASRKPSWVAKAAFHIVTRNSWEDTLQGVRLATNVA
                     ERLRDDGVLPHCPPASAGWHPIHPFPGCAEERRSILVEAMQLTSSGERAQMIASAPTC
                     E"
     gene            complement(1932..>2173)
                     /locus_tag="VF516_29265"
     CDS             complement(1932..>2173)
                     /locus_tag="VF516_29265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111871.1"
                     /translation="DRDRRDWTRGVSLATGVVERLRDDGLLPHCPVAGAEWPDVDPCV
                     EQRRRANEEGPLRPDETRELRGLDPAPMCNQGGGR"
BASE COUNT          298 a          789 c          748 g          338 t
ORIGIN      
        1 gtgctcgacc caacgggcca cgagctcgcg ggcctcctcg agcgtcttgg gctggccggg
       61 ccgaatggcg ttctccttga tggtcttgtg atagcgctcg agtttgccgt tcgactgcgg
      121 gtagtacggt gcggtccgga catgggtcat gccggtgagg cgaatgaaca cttcgagcca
      181 gtcgctcacg ttgtctgggc cggtcggtcc gacgagctcg ggatgcttcg caatgatctc
      241 atcgaggcgc cgccgcgccg cagcgagccc cacctcgtcg atcggctgat cgtcgtcatc
      301 atcatcgcca cgcgtcatcc ggcgaccata cgcgcaacct gcaccggcgc taagggtcgg
      361 ccgttgcgtg cgcgcaggcc cttgcggtcg agctcctcgg cgaccttgcg gagggatagc
      421 ccggtggcgc gatgcgcgcg cgcctcggcg atcacggctt gctcaatcgc gacctcgacg
      481 agcgtcacac cgtcggccgc gagcgcgtag ccgtaggacg ccgcgccgcc ggtgtactcg
      541 ccctgtgatg ccttgtgctg catcgccgcc gcggtccgct cgccgatcgc ctcgcgctcc
      601 cactgcccga tcaccgtgag catgttcagc accatgcccc cgccgcgctg cgggtgtcga
      661 cctgctcgcc caccgacagc agcgcgtgct tgccgtctcg gaagtaccga tccacgaggt
      721 cgcagaggtc gcgcaccgag cgggtgaggc ggtggagctt gaccacgagg agcgcatcgg
      781 ccttgccggt gcgcagcatc gccagcgcga gatcgagcgc gggccgcgcc agcgacttgg
      841 ccgactagcc cgggtcaacc tcgatcgcca cgaggttgag atcgaacagg gccgcgtagg
      901 cttcgacctt ggcacgctgc gcctccaggc tcacgccgcg accggcctgc ttgtccgtgg
      961 agactcggag gtaggcgacc gtgcgcgtgc gggcgtcgct catgcactac ctgctgcgtc
     1021 gtgcggttta cgttgtcaac catcgtgtgg gtcctggtgt aacggcgggt ccccgtcgtt
     1081 acaccccggc ccgcgatcgg cggcggatcc gagccgcgcc acgacgcgcc cgcgtgatcg
     1141 cgagcactcg ccagccccca ccgccacccg gaccgcgcgc tccgggccgg gccaggaacg
     1201 ccagcgccgc cgatgcgatc gccgacagcg cgctcccgat cgcatcgggt cggcggctca
     1261 ctcgcaggtc ggcgcagacg cgatcatctg cgcgcgctcg ccgctgctgg tgagctgcat
     1321 cgcctcgacc aggatactgc ggcgctcctc ggcgcagccg gggaacgggt ggatcgggtg
     1381 ccaaccggcg ctggcgggcg ggcagtgcgg gaggacgccg tcgtcgcgca gccgctcggc
     1441 gacgttcgtc gctagccgga cgccctggag cgtgtcctcc cagctgttgc gggtcacgat
     1501 gtggaacgcc gccttggcga cccagctcgg cttgcgcgac gcggcgtcga gcaactggaa
     1561 cccgaagtac agcgtgccgc tcttgtcgtt gtggttgcct tcgatcttga ccagatcgct
     1621 gccctcgctg cgcaccacca gcgcggtgct aggctggaag ccgcccgcgc tatgaagggc
     1681 atcgagcggg tccgcagcga taggaccgac gacaacatgg ctcgccacgc cgcacgccgc
     1741 gagccggttc tcgaggccgc cgatgaagcc gcgcgacacg cgtccatcga cgtcgaggtg
     1801 cgcgatcacg agcaggcgct cgatcggcgc ggtgttcgcg aggtcggccc gcgagcgaaa
     1861 gatcgtcgcc ggcggctcgc agccagcaag cgcgattgtc gccgcagcga ccaccatgcc
     1921 ggtcctccgc gtcaccggcc gccgccctga ttgcacatcg gcgcggggtc gagtccgcgc
     1981 aactcccggg tctcgtcggg ccgcagcgga ccctcctcgt ttgcccgccg gcgctgctca
     2041 acgcacgggt cgacgtcggg ccactccgca cccgccactg ggcagtgcgg tagcaggccg
     2101 tcgtcgcgca gccgctccac cacgccggtc gcaaggctca cgccccgggt ccagtcgcgg
     2161 cgatcgcgat cgg
//
LOCUS       DASVOR010001557         2754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_278084,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001557 DASVOR010000000
VERSION     DASVOR010001557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278084"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="VF516_29270"
     CDS             <1..406
                     /locus_tag="VF516_29270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002855962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HEX8111872.1"
                     /translation="ALYNGRIRRGEHMRVFPLSNWTELDVWQYIALEDIPLPSIYFAH
                     QRDVFERDGMLYAANPHVKLLPGERIRPEVVRFRTIGDMTCTGAVPSDARTLEAVIAE
                     VAAARITERGATRADDRFTEASMEDRKREGYF"
     gene            406..1767
                     /locus_tag="VF516_29275"
     CDS             406..1767
                     /locus_tag="VF516_29275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017488255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8111873.1"
                     /translation="MSDTSDRPGERLSERLGERLSERLAVSRRPVVGVADDAELLRFS
                     TAGSVDDGKSTLIGRLLYDTKTVFEDQVRHVEDVSRRRGDERVNLALLTDGLRAEREQ
                     GITIDVAYRYFATRKRKFIIADTPGHIQYTRNMVTGASTASLAIILVDARHGMVEQSR
                     RHAFIASLLRIPHLVFAVNKMDLVDWSEARFEEIEDEFRNFAARLDVQDIAFIPISAL
                     TGANVVEHSRDTPWYHGPTLLYHLETVHIASDRNLIDVRFPVQRVIRPESAEFPDYRG
                     YAGQVAGGVLRPGDEVVALPSGLATRITRIEQLGEDLDAAFAPMSVTIHLEDDLDVSR
                     GDMLCRPNNQPMVGQDLDAMVCWMSERPLLSRGRYAIKHTTRWGRAVVNDILYRLDVN
                     TGHRDDTAKELKLNDLGRIRLRTTTPILYDPYRRNRVTGSFILVDEANHTTVAAGMLL
                     APE"
     gene            1775..>2754
                     /locus_tag="VF516_29280"
     CDS             1775..>2754
                     /locus_tag="VF516_29280"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8111874.1"
                     /translation="MTRTGAHRVAVLGCGDWGKNLVRNFHALGALAAVADVSPNGQKA
                     AARIAPGAAIHDRLDAALDDRSIAGIVIGTPAETHAELTERALLAGKDVLCEKPLALT
                     YDAARRVHDLAIRRDRVLMVGHLLEYHPAIQELRALVAGGRLGQIRYLYSNRTNLGKV
                     RREENILWSFAPHDIAVILRLVGALPCQVIAAGGAYLQADIADVTVTQLLFDSGIAAH
                     VFVSWLNPFKEQKLVVVGSERMATFDDVRRELVLHDQHVDLQDGEPVPVKGAGVAIPY
                     AADEPLRIECEAFLAAIASRTAPLTDSASALRVLRVLEAAQRSLITRGRPV"
BASE COUNT          418 a         1029 c          932 g          375 t
ORIGIN      
        1 ggcgctgtac aacggccgga tccggcgcgg cgagcacatg cgggtgttcc cgctgtcgaa
       61 ctggaccgag ctcgacgtct ggcagtacat cgcgctcgag gacatcccgc tgccgtcgat
      121 ctacttcgcg caccagcgcg acgtgttcga gcgcgacggc atgctgtacg cggccaaccc
      181 acacgtcaag ctcttgcccg gcgagcggat ccgccccgag gtcgtccggt tccggaccat
      241 cggcgacatg acctgcaccg gcgcggtgcc gtcggacgcc cgcacgctcg aggcggtgat
      301 cgccgaggtc gcggcggcgc ggatcaccga gcgcggcgcg acccgggccg acgatcgctt
      361 caccgaggcc tcgatggagg accgcaagcg cgagggctac ttctgatgag cgacacgagt
      421 gaccgcccgg gggaacgatt gagcgagcgc ctgggggaac gattgagcga gcggctggcc
      481 gtcagccggc ggccggtcgt cggtgtcgcc gacgacgccg agctgctccg gttctcgacc
      541 gcgggctcgg tcgacgacgg caagtcgacc ctgatcgggc ggctcctgta cgacaccaag
      601 accgtgttcg aggatcaggt ccgccacgtc gaggacgtgt cgcggcggcg cggcgacgag
      661 cgggtcaacc tggcgctgct caccgacggc ctgcgcgccg agcgcgagca gggcatcacg
      721 atcgatgtcg cctaccgcta cttcgcgacc cgcaagcgca agttcatcat cgcggacact
      781 ccgggccaca tccagtacac ccgtaacatg gtcaccggcg cgtcgaccgc gagcctggcg
      841 atcatcctgg tcgacgcccg ccacggcatg gtcgagcaga gccgccgcca cgcgttcatc
      901 gcgtcgctgc tccgcatccc gcacctggtg ttcgcggtca acaagatgga cctggtcgac
      961 tggagcgagg cgcggttcga ggagatcgag gacgagttcc gcaacttcgc ggcccggctc
     1021 gacgtccagg acatcgcgtt catcccgatc agcgcgctga ccggggccaa cgtcgtcgag
     1081 cactcgcgcg acacgccgtg gtaccacggg ccgaccctgc tctatcacct cgagaccgtg
     1141 cacatcgcca gcgatcgcaa cctgatcgac gtgcggttcc cggtccagcg cgtgatccgc
     1201 cccgagtccg ccgagttccc ggactaccgc ggctacgccg gccaggtcgc cggcggcgtg
     1261 ctgcggccgg gcgacgaggt cgtcgcgctg ccgagcgggc tggcgacccg gatcacccgg
     1321 atcgagcagc tcggcgagga cctcgacgcg gcgttcgcgc cgatgagcgt gacgatccac
     1381 ctcgaggacg acctcgacgt gtcgcgcggc gatatgctgt gccggcccaa caaccagccg
     1441 atggtcggcc aggatctcga cgccatggtg tgctggatgt ccgagcggcc gctcctgtcg
     1501 cggggccgct acgcgatcaa gcacaccacg cggtggggcc gcgcggtggt caacgacatc
     1561 ctctaccggc tcgacgtcaa caccggccac cgcgacgaca cggccaagga gctcaagctc
     1621 aacgacctcg gccggatccg gctgcgcacg accacgccga tcctctacga cccgtaccgc
     1681 cgcaaccggg tgaccggctc gttcatcctg gtcgacgagg ccaaccacac cacggtcgcc
     1741 gcggggatgc tgctggcccc cgagtgagcg cgcggtgacg aggaccggcg cgcaccgggt
     1801 cgccgtgctg ggctgcgggg actggggcaa gaacctggtc cgcaacttcc acgcgctcgg
     1861 cgcgctcgcc gcggtcgccg acgtctcgcc caacggccag aaggccgccg cgcggatcgc
     1921 ccccggcgcc gcgatccacg accggctcga cgccgcgctc gacgaccgct cgatcgccgg
     1981 catcgtgatc ggcacgcccg ccgagaccca cgccgagctc accgagcgcg cgctgctcgc
     2041 cggcaaggac gtcctgtgcg agaagccgct cgcgctgacc tacgacgccg cccgccgcgt
     2101 ccacgacctc gcgatccgcc gcgaccgggt cctgatggtc ggccacctgc tcgagtacca
     2161 ccccgcgatc caggagctcc gcgcgctggt cgccggcggc cggctcggcc agatccgcta
     2221 cctgtactcc aaccgcacca acctcgggaa ggtccgccgc gaggagaaca tcctgtggtc
     2281 gttcgcgccg cacgacatcg ccgtgatcct tcggctggtc ggcgcgctgc cgtgccaggt
     2341 gatcgcggcc ggcggcgcct acctccaggc cgacatcgcc gacgtcaccg tgacccagct
     2401 cctgttcgac agcggcatcg ccgcccacgt gttcgtgtcg tggctcaacc cgttcaagga
     2461 gcagaagctg gtggtggtcg gctccgagcg gatggcgacg ttcgacgacg tccgccgcga
     2521 gctcgtgctc cacgaccagc acgtcgacct ccaggacggc gagccggtcc cggtcaaggg
     2581 cgccggggtc gcgatcccgt acgccgccga cgagcccctg cgcatcgagt gcgaggcgtt
     2641 cctcgccgcg atcgcctcgc gcacggcgcc gctgaccgac agcgcgagcg cgctgcgcgt
     2701 gctgcgcgtg ctcgaggcgg cgcagcgctc gctgatcacc cgcggccggc ccgt
//
LOCUS       DASVOR010001558         1532 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_308442,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001558 DASVOR010000000
VERSION     DASVOR010001558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308442"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..160
                     /locus_tag="VF516_29285"
     CDS             <1..160
                     /locus_tag="VF516_29285"
                     /inference="COORDINATES: protein motif:HMM:NF014912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HEX8111875.1"
                     /translation="ETYEYTQMYPGFAKAAREEGFEEIAEWFETLARAERSHAGRFQK
                     GLDGLMTK"
     gene            224..808
                     /locus_tag="VF516_29290"
     CDS             224..808
                     /locus_tag="VF516_29290"
                     /inference="COORDINATES: protein motif:HMM:NF023432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HEX8111876.1"
                     /translation="MSLVRPITRQDIKGPKLYGPIRDDYRKRVIAMKRTRRILVGDRV
                     SLVFENRHTLALQIEEILRAESLTREDQIEDEIAIYNQLMPTESSLSATLFIELPGEI
                     DPYAALKQLIGLDEHVVLHIGPHTVRAAFEPGRATEDKISAVQYTRYPVSREVRAALE
                     TPGTAIAVEIDHPNYRHRVTCTEATRASLAADYV"
     gene            801..1397
                     /locus_tag="VF516_29295"
     CDS             801..1397
                     /locus_tag="VF516_29295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111877.1"
                     /translation="MSSRRDTAAAALAAATWAATWAATWGAIWGATWLAACGGGQREQ
                     IEREARRFDCRGRRASYMVAHHLAGDEIGVQIDCADAGPRIKRWRTDRSGTHQEDARS
                     LTPGEFDKVWREIDGTGWPNLRDCSNGTGGKHDPIYVFDVKDDQNQASFQCQSQSAPY
                     PYNGIVDPLDLAAQQGRRQLGDDEPADLKALDRKDKQR"
BASE COUNT          264 a          558 c          523 g          187 t
ORIGIN      
        1 cgagacctac gagtacacgc agatgtaccc gggcttcgcc aaggccgcgc gcgaggaggg
       61 cttcgaggag atcgccgagt ggttcgagac cctggctcgc gccgagcgct cgcacgccgg
      121 ccggttccag aagggcctcg acggcctgat gacgaagtga tgacgaagtg acggcgaagt
      181 agcgcgcgac cctcggcacg ccggtgcatg gtacacccga gccatgagtc tggtgcgacc
      241 catcacccgc caggacatca agggccccaa gctctacggg ccgatccgag acgactaccg
      301 caagcgcgtg atcgcgatga agcggacgcg ccgcatcctg gtcggtgatc gcgtgtcgct
      361 ggtgttcgag aaccggcaca cgctggcgct ccagatcgag gagatcctgc gggccgagag
      421 cctgacccgc gaggaccaga tcgaggacga gatcgcgatc tacaaccagc tgatgccgac
      481 cgagagctcg ctgtcggcga cgctgttcat cgagctgccc ggcgagatcg atccctacgc
      541 cgcgctcaag cagctgatcg gcctcgacga gcacgtcgtg ctccacatcg ggccgcacac
      601 ggtccgcgcc gcgttcgagc ccggccgcgc gaccgaggac aagatctccg ccgtgcagta
      661 cacgcgctat ccggtgagcc gcgaggtgcg cgccgcgctg gagaccccgg gcaccgcgat
      721 cgcggtcgag atcgatcacc cgaactatcg ccaccgcgtc acctgcaccg aggcgacccg
      781 cgcctcgctc gccgccgact atgtctagcc ggcgcgacac cgcggccgcc gcgctcgccg
      841 cggccacctg ggcggccacc tgggcggcca cctggggggc catctggggg gccacctggc
      901 tcgccgcgtg cggcggcggg cagcgcgagc agatcgagcg cgaggcccgg cggttcgact
      961 gccgcggccg gcgcgcgagc tacatggtcg cccaccacct ggccggcgac gagatcggcg
     1021 tccagatcga ctgcgccgac gccgggccgc ggatcaagcg ctggcggacc gatcggtccg
     1081 gcacccacca ggaggacgcc cgcagcctga cgcccggcga gttcgacaag gtgtggcgcg
     1141 agatcgatgg caccggatgg cccaacctgc gcgactgcag caacggcacc ggcggcaagc
     1201 acgatccgat ctacgtgttc gacgtcaagg acgatcagaa ccaggcatcg tttcaatgtc
     1261 agtcgcagtc ggcgccctat ccgtataacg gtatcgtcga tccgctggat ctcgcagccc
     1321 agcaaggccg ccggcagctc ggcgacgacg agcccgccga cctgaaggcg ctcgaccgca
     1381 aggacaagca gcggtgagcg gagtcgccac gccgcacccg gcgcgccccc gtgccgcatc
     1441 gcgaggcacc cggtgaccgg accgctggcc ggcaccaccg ccgtcgtcac cggcgccagc
     1501 cgcggcatcg gtcgcgcgat cgcgctccgg ct
//
LOCUS       DASVOR010001559         3408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_337126,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001559 DASVOR010000000
VERSION     DASVOR010001559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3408
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337126"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="VF516_29300"
     CDS             <1..1391
                     /locus_tag="VF516_29300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111878.1"
                     /translation="ILGAGPAHDSFHTIADYLDELCFGIHYQEEEPGFVDLFALMDPA
                     TGTGHPLLGPLRALEHLRARHLRRQMDTVSAAAFGAGAVAWWNSVLHMVPFGTNWSNP
                     TENPLTGDEPGPVNPLLSGATRPRAIKEYGYAELKEGKAQTGFSEFDLTQAALARARM
                     ATPAETDTMLPFPVLLAIMKTEGVQQFGPLNRIVKNTTVRAGTVNWNGWRTPLTTANF
                     VFGDDARRGRQLMLLWPYGLDHFSHYAGGDGIVPPRTFNDGLVDDLANTTPPISPILT
                     MEPGEDPGRYISARVTSELVPTVAPLANVFIWKRARRVHWAILGLACGYFRLMEAQIL
                     AGKNGFVSPDPAYLTPPPSLAGKTPLDVEYRDYITYYAMIYLAYNAGASVWRPALATA
                     EAGRGATPLGRRDFLFFRSNRELQGMVHCCHFMIALDAFLRIDYLDGKQPVDFAAASG
                     YSGSVAGRAWGV"
     gene            1391..>3408
                     /locus_tag="VF516_29305"
     CDS             1391..>3408
                     /locus_tag="VF516_29305"
                     /inference="COORDINATES: protein motif:HMM:NF042646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6603 domain-containing protein"
                     /protein_id="HEX8111879.1"
                     /translation="MASGSLDALIVPVAEAVLALEDLLAAANGGGALLGQLGYALPAA
                     ANAEPLFTDVAAKVASLAASAAAVAQAYADGTWQNASFLPKVLALANAVAASIETAEQ
                     LPAKAQTVFAAVPDFLAHAPLDELPRRLVDRLIVVYLRKERPRVAAVLSLLALTSDEY
                     VPADDFNPDYRRLRVEWERIPRWFTAPGQVLADAYGWGSAAFNSSDVLERLDTMLALF
                     GVMALADPLPDALAPLAPPAERLQLPLFSGTVDVPPDGLAGAVLGLRIGRAETAADRD
                     DAGLAITPYADGAAGVSFDLAPGWTLAAKGALDAEGLAVVIQPHTGVRLEAAAAISAE
                     VGVTLARDGSALGPLILFGSAGATRLEIAQIALGLVASTSPAGQDASIELGLHGISLV
                     VQAGEGDGFLKVVLPADPLEIKFDLTLGASAKRGIYFSGGAGFEYTFHVNAQLGPIAI
                     DTIDLAIAVTPTALDVVATTTGALELGPLTAVVEKIGLDTKLSFGQPGNLGRANLGFG
                     FHPPTGLGLSIDASVVSGGGFLSFDVEKQQYSGILQLSIQDVVDVKAIGILNARLPDG
                     RPGYSLLLIITGEFPPIQLGMGFTLNGVGGLIAINRTVVVDVLRAGVRDGGLDAILFP
                     PDPLSHVASLLGTLSAVFPVAENRYVFGPMVRIGWGSPTLITLDLALIL"
BASE COUNT          477 a         1225 c         1185 g          521 t
ORIGIN      
        1 cgatcctcgg cgcggggccg gcgcacgaca gcttccacac gatcgccgac tacctggacg
       61 agctgtgctt cggcattcac tatcaggagg aggagcccgg tttcgtcgat ctgttcgcac
      121 tcatggatcc ggcaacgggc acggggcacc cgctgctcgg tccgctgcgc gcgctcgagc
      181 acctgcgggc gcgccacctc cgccgccaga tggacaccgt gtcggccgcg gcgttcggcg
      241 ccggcgcggt cgcgtggtgg aacagcgtgc tccacatggt cccgtttggc acgaactgga
      301 gtaatccaac ggagaatccg ctgaccggcg acgagccggg cccggtcaat ccgctgctct
      361 ccggcgccac acggccgcgc gcgatcaagg agtacggcta cgcggagctg aaggagggga
      421 aggcgcagac gggattcagc gagttcgacc tcacccaggc ggcgcttgcg cgcgcccgca
      481 tggcgacgcc tgcagagacg gacacgatgc tcccattccc ggtgctcctc gcgatcatga
      541 agaccgaggg cgtgcagcag ttcggcccgc tcaaccgcat cgtcaagaac acgacggtgc
      601 gcgcgggcac ggtgaactgg aacggctggc ggacgcccct caccacggcg aacttcgtgt
      661 tcggcgacga cgcgcgccgt gggcgccagc tcatgctgct ctggccgtac gggctcgatc
      721 actttagcca ctacgcgggc ggcgacggca tcgtaccccc caggacgttc aacgacgggc
      781 tcgtcgacga cctcgcgaac accacaccgc ccatctcgcc gatcctcacg atggagcccg
      841 gcgaggatcc gggcagatac atcagcgcgc gcgtgacgag cgagctcgtg ccgacggtgg
      901 cgccgctcgc caacgtcttc atctggaagc gcgcgcgccg cgtgcactgg gcgatcctcg
      961 ggctcgcgtg cggctacttc cgcctgatgg aggcgcagat cctcgccggc aagaacggct
     1021 tcgtctcgcc cgatccggcc tacctcacgc cgccgccgtc gctcgcgggc aagacgccgc
     1081 tcgacgtgga gtaccgcgac tacatcacgt actatgcgat gatctacctc gcgtacaacg
     1141 cgggtgcgag cgtgtggcgc cccgcactcg cgaccgcgga ggccgggcgc ggcgcgacgc
     1201 cgctcgggcg gcgcgacttc ctgttcttcc gcagcaaccg cgaactccag ggcatggtgc
     1261 actgctgcca cttcatgatc gcgctcgacg cgttcttgcg catcgactac ctcgacggca
     1321 agcagcccgt cgacttcgcg gcggcgagtg gctacagcgg gtccgtggct ggccgcgcgt
     1381 ggggcgtgtg atggcgagcg gatcgctcga cgcgctgatc gtgcccgtcg ccgaggcggt
     1441 gctcgcgctc gaggacctgc tcgcggcggc gaacggtggc ggggcgctgc tcgggcagct
     1501 cggatacgcg ctgcccgcgg cggcgaacgc ggagcctctg ttcaccgacg tcgccgcgaa
     1561 ggtggcgagc ctcgcggcct cggcggcggc ggtcgcgcag gcatatgccg acggcacgtg
     1621 gcagaacgcg agcttcttgc cgaaggtgct cgcgctcgcg aacgcggtcg ccgcctcgat
     1681 cgagacggcg gagcagttac cggcgaaggc gcagacggtg ttcgccgcgg tgccggactt
     1741 cctcgcgcac gcgccgctcg acgagctgcc gcgccgcctc gtcgatcgac tgatcgtcgt
     1801 gtacctgcgc aaggagcgcc cgcgcgtcgc ggcagtgctg tcgctgctcg cgctcacgtc
     1861 cgacgagtac gtgccagcgg acgacttcaa ccccgattac cggcggctgc gcgtcgagtg
     1921 ggagcgcatc ccgcggtggt tcacggcgcc cgggcaggtg ctcgccgacg cgtacggctg
     1981 gggcagcgcc gcgttcaact cgagcgacgt gctcgagcgg ctcgacacga tgctcgcgct
     2041 gttcggcgtg atggcgctcg cggatccgct gcccgacgcg ctcgctccgc tcgcgccgcc
     2101 agcggagcgc ctccagctcc cgctgttcag cggcaccgtc gacgtgccgc ccgacgggct
     2161 cgcaggcgcg gtgctcgggc tccgcatcgg tcgcgcggag acggcggcgg atcgcgacga
     2221 cgccggcctc gcgatcaccc cgtacgccga cggcgcggcg ggcgtgtcgt tcgacctcgc
     2281 gcccggctgg acccttgccg cgaagggagc gctcgacgcc gagggcctcg cggtcgtcat
     2341 tcagccgcac accggcgtgc ggctcgaggc cgccgccgcc atctccgcgg aggtcggcgt
     2401 cacgctcgca cgcgacggct ccgcgctcgg gccgctcatc ctgttcggta gcgcgggcgc
     2461 cacgcggctc gagatcgcgc agatcgcgct cgggctcgtc gcgtcgacgt ccccggcggg
     2521 gcaggacgcg agcatcgagc tcggcttgca cggcatctcg cttgtcgttc aggccggcga
     2581 gggcgacggc ttcttgaagg tcgtgctccc cgcggatccg ctcgagatca agttcgacct
     2641 cacgctcggc gcgagcgcga agcgcggcat ctacttcagt ggcggcgccg ggttcgagta
     2701 cacgttccat gtcaatgcgc agctcgggcc gatcgccatc gacacgatcg atctcgcaat
     2761 cgccgtcacg ccgaccgcgc tcgacgtcgt cgccacgacg acgggcgcgc tcgagctcgg
     2821 cccgctcacc gcggtcgtcg aaaagatcgg cctggacaca aagctctcat tcggccagcc
     2881 cggcaatctc ggccgggcga acctcgggtt cggcttccat ccgccgaccg gcctggggct
     2941 gtcgatcgac gcctcggtgg tatcgggcgg cgggttcctg tccttcgacg tcgagaagca
     3001 gcagtacagc ggcatcctcc agctcagcat ccaggacgtc gtcgacgtca aggcgatcgg
     3061 catcctcaat gccaggctgc ccgacggccg gcccggctac tcgctgctgc tgatcatcac
     3121 cggcgagttc ccgccgatcc agctcgggat gggcttcacg ctcaacgggg tcggcgggct
     3181 gatcgcgatc aaccgcaccg tggtggtgga cgtgctgcgc gcgggcgtgc gcgacggcgg
     3241 gctggacgcg atcctgtttc cgcccgatcc gctgagccac gtggcgagcc tgctcggcac
     3301 gctgagcgcg gtgtttccgg tggcggagaa ccgctacgtg ttcgggccga tggtccggat
     3361 cggctggggc tcgcccaccc tcatcacgct cgacctggcc ctgatcct
//
LOCUS       DASVOR010001560         1981 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_424461,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001560 DASVOR010000000
VERSION     DASVOR010001560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424461"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF516_29310"
     CDS             <1..327
                     /locus_tag="VF516_29310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111880.1"
                     /translation="LAPGERDGLGARTGWSLRDLVHQKLGAGARVAALVDEDGHRVEL
                     DAAAWDSAVTTPILRLNRRGRLRFHWVGVRNRLPGAKGVQRIEIVLDEPAGAATSSPS
                     VRQTRP"
     gene            complement(312..1673)
                     /gene="gdhA"
                     /locus_tag="VF516_29315"
     CDS             complement(312..1673)
                     /gene="gdhA"
                     /locus_tag="VF516_29315"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEX8111881.1"
                     /translation="MKDYVPALMSDVKAKNPAEPEFHQAVQEVAESLAIVLDRHPEYR
                     HNKILERIIEPERVMMFRVPWDDDRGTVQINRGFRIEMNSAIGPYKGGLRFHPSVNLG
                     ILKFLAFEQVFKNSLTTLPMGGGKGGSDFDPKGKSDHEVMRFCQSFMTELARHIGPNT
                     DVPAGDIGVGGREIGYLFGQYKRLRNEFTGVLTGKGLNWGGSLIRPEATGYGAVYFAA
                     EMLATRGETLEGKTCLVSGSGNVAQYTVEKLLDLGARPVTLSDSSGYIYDEAGVDRDK
                     LKFVMTLKNARRGRIEEYAAAYKSAVYVPAHHALDHNPLWTHKAHCAFPSATQNEING
                     KDAANLLRNGVYLVAEGANMPTNLDGVAQFVNAKILYGPGKAANAGGVAVSGLEMAQN
                     SMRYSWLREEVDDRLRVIMRSIHKACTTTAERFGVAGNYVAGANIAGFLKVADSMMDQ
                     GLV"
     gene            complement(1774..>1981)
                     /locus_tag="VF516_29320"
     CDS             complement(1774..>1981)
                     /locus_tag="VF516_29320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111882.1"
                     /translation="LPEVGPRGTTLGILGMRERAWRLGGECTVKRRTPRGTTVAVVVP
                     LRFPAEHASDDGHGPGMDPALPRR"
BASE COUNT          261 a          687 c          706 g          327 t
ORIGIN      
        1 ctcgcgcccg gcgagcgcga cggactgggc gcgcgcaccg gctggtcgct gcgcgacctc
       61 gtccaccaga agctcggcgc cggcgcccgc gtggcggcgc tcgtcgacga ggacggccat
      121 cgcgtcgagc tcgacgccgc ggcctgggac agcgccgtta cgacgccgat cctgcgcctc
      181 aaccgcagag gccggctccg cttccactgg gtcggcgtgc gcaaccggtt gcccggtgcg
      241 aagggcgtgc agcggatcga gatcgtgctc gacgagccgg ccggcgctgc tacgtcttca
      301 ccgagcgtcc gtcagaccag gccctgatcc atcatcgagt cggcgacctt gaggaacccg
      361 gcgatgttgg cgcccgcgac gtagttgccc gcgacgccga accgctcggc ggtggtggtg
      421 cacgccttgt ggatgctgcg catgatcacg cgcagccggt cgtcgacctc ctcgcgcagc
      481 cagctgtagc gcatgctgtt ctgcgccatc tcgagaccgg acaccgcgac gccgccggcg
      541 ttggccgcct tgcccgggcc gtacaggatc ttcgcgttga cgaactgggc gacgccgtcg
      601 aggttggtcg gcatgttggc gccctcggcg accaggtaga ccccgttgcg caagaggttc
      661 gccgcgtcct tgccgttgat ctcgttctgg gtcgccgacg ggaacgcgca gtgcgccttg
      721 tgggtccaca gcgggttgtg gtcgagcgcg tggtgggccg ggacgtagac cgcggacttg
      781 taggccgcgg cgtactcctc gatccggccg cgccgcgcgt tcttgagcgt catgacgaac
      841 ttgagcttgt cgcgatcgac gccggcctcg tcgtagatgt agcccgacga gtccgacagc
      901 gtgaccgggc gcgcgccgag gtcgagcagc ttctcgaccg tgtactgcgc gacgttgccg
      961 ctgcccgaaa ccaggcaggt cttgccctcg agggtctcgc cgcgggtcgc cagcatctcg
     1021 gcggcgaagt acaccgcgcc gtagccggtg gcctcgggcc ggatcagcga gccgccccag
     1081 ttgaggccct tgccggtcag cacgccggtg aactcgttgc gcagccgctt gtactggccg
     1141 aacaggtagc cgatctcgcg gccgccgacg ccgatgtcgc cggccggcac gtcggtgttg
     1201 gggccgatat ggcgcgccag ctcggtcatg aagctctggc agaaccgcat cacctcgtga
     1261 tcgctcttgc ccttggggtc gaagtccgag ccgcccttgc cgccgcccat cggcagcgtg
     1321 gtcagcgagt tcttgaacac ctgctcgaac gcgaggaact tgaggatgcc gaggttgacc
     1381 gaggggtgga accgcaggcc gcccttgtac gggccgatcg cgctgttcat ctcgatccgg
     1441 aacccgcggt tgatctggac cgtgccgcgg tcgtcatccc acgggacgcg gaacatcatc
     1501 acgcgctccg gctcgatgat gcgctccagg atcttgttgt ggcggtactc cgggtggcgg
     1561 tcgaggacga tcgcgagcga ctccgcgacc tcctggaccg cctggtggaa ctccggctcc
     1621 gccgggttct tggctttgac gtcggacatc agcgccggga cgtagtcctt catggccgcg
     1681 accatagtcg aagcgctcgc cggcgccagg tctgcggggc gttctaccct gcggcggcat
     1741 ggcgcgccgc gtgccggagc gtggcgcgct gcgtcagcgc cggggcagcg ccgggtccat
     1801 gccgggtcca tgaccgtcgt cgctcgcgtg ctccgccggg aaccgcagcg ggaccacgac
     1861 cgcaaccgtg gtgcctcggg gcgtgcggcg cttgaccgtg cactcgccgc cgagtcgcca
     1921 cgcgcgctcg cgcatgccga ggatgccgag cgtggtgccg cgcgggccga cctcgggcag
     1981 g
//
LOCUS       DASVOR010001561         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_542114,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001561 DASVOR010000000
VERSION     DASVOR010001561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542114"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="eutC"
                     /locus_tag="VF516_29325"
     CDS             <1..805
                     /gene="eutC"
                     /locus_tag="VF516_29325"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HEX8111883.1"
                     /translation="PPARVYSPRPERSAFAAEAARHTTALLGIGHVGTRYATDVVLQF
                     QAELAVAHAAVGAELPDGWAEAHGFVGLTTRVSSHREFLLRPDLGRRLSDASLEVVRT
                     RCTRGADVQLIVADGLSAVACVQTGKQLHDAVAAACRARGLSVGTPVVARFARVWLED
                     EIGQETGAKVAMILLGERPGLGTGDGLSAYVVYEPRIGKTDGDRNMISNIHARGTPPD
                     EAAARLAALAAAMIDQRISGVTLELGRLGEAAGGRGYRAPQVRQRLVNA"
     gene            802..1455
                     /gene="eutL"
                     /locus_tag="VF516_29330"
     CDS             802..1455
                     /gene="eutL"
                     /locus_tag="VF516_29330"
                     /inference="COORDINATES: protein motif:HMM:NF011934.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HEX8111884.1"
                     /translation="MILEPIVPTVLSVRRLPGADPALIAAYGGDPARHTALGLVTCDQ
                     DDAMYVALDEATKHAPVDVIFARSFYAGSAHASGRLSGEILGVLAAAEPQAVEHGLAA
                     LLGCLAHDACFYDADGKRTVTVFPHVIASLGEYLSREAGLPPGAAMAYLVAPPIEATI
                     GLDAALKAADVRAVRVVPPPTETNFACAWLTGSLDACEAAALAYAAAVVDVALAPKR"
     gene            1546..2346
                     /locus_tag="VF516_29335"
     CDS             1546..2346
                     /locus_tag="VF516_29335"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8111885.1"
                     /translation="MATETKRKFAVVTGASSGIGFELARQCLEHGFDVLICAEDAAID
                     GAAKQLETAGALVIPFQADLATYDGVERLYQAIRDQHRPVDALLLNAGIGVGGEFIKT
                     DLAADLRTIALNCNHTVHLAKRVLPDMVSRRQGRVLMTGSIVSTSPNPYQAVYGATKA
                     FVMSFGEALRYELKDTGVTVTVLQPGVTDTRFFERADLRDTKIGQSSKDDPALVARQG
                     FDAMMAGKDSVLAGSLKSKVEGLMNELLPETVKAAQAAKRTRPGSAKQ"
     gene            2387..>2489
                     /locus_tag="VF516_29340"
     CDS             2387..>2489
                     /locus_tag="VF516_29340"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HEX8111886.1"
                     /translation="MAEPLRAAVIGSGFGGLAAAIRLAAMGVRVTLFE"
BASE COUNT          336 a          956 c          871 g          326 t
ORIGIN      
        1 gccgcccgcc cgggtgtact cgcccaggcc ggagaggtcc gcgttcgccg ccgaggccgc
       61 gcgccacacc accgcgctgc tcgggatcgg ccacgtcggc acgcgctacg ccaccgacgt
      121 cgtgctccag ttccaggccg agctcgccgt cgcgcacgcc gcggtcggcg ccgagctgcc
      181 ggatggctgg gccgaggccc acggcttcgt cggcctgacc acccgggtga gctcgcaccg
      241 cgagttcctg ctgcggcccg acctcgggcg ccggctgtcg gacgcgtcgc tcgaggtcgt
      301 gcgcacccgg tgcacccgcg gcgccgacgt ccagctgatc gtcgccgacg gcctgtcggc
      361 ggtcgcctgc gtccagaccg gcaagcagct gcacgacgcc gtcgccgcag cgtgccgggc
      421 gcgcggcctg tcggtcggca cgccggtcgt ggcgcggttc gcgcgggtct ggctcgagga
      481 cgagatcggc caggagaccg gcgccaaggt cgcgatgatc ctgctcggcg agcggcccgg
      541 gctcggcacc ggcgacggcc tgtcggcgta cgtcgtgtac gagccgcgga tcggcaagac
      601 cgacggcgac cgcaacatga tctccaacat ccacgcgcgc ggcaccccac ccgacgaggc
      661 agccgcgcgg ctcgcggcgc tcgcggccgc catgatcgac cagcggatca gcggcgtgac
      721 cctggagctc ggccggctcg gcgaggccgc cggcggccgc ggctaccgcg ccccgcaggt
      781 ccggcagcgc ctggtgaacg catgatcctc gagccgatcg tcccgaccgt gctctcggtg
      841 cgccgcctgc cgggcgccga tccggcgctg atcgcggcgt acggcggcga tccggcgcgc
      901 cacaccgcgc ttggcctggt gacctgcgac caggacgacg cgatgtacgt cgcgctcgac
      961 gaggcgacga agcacgcgcc cgtcgacgtg atcttcgcgc ggagcttcta cgccggcagc
     1021 gcccacgcca gcggccggct gtccggcgag atcctcggcg tgctcgccgc cgccgagccc
     1081 caggccgtcg agcacggcct cgccgcgctg ctcggctgcc tcgcccacga cgcgtgcttc
     1141 tacgacgccg acggcaagcg caccgtcacc gtgttcccgc acgtgatcgc gtcgctcggc
     1201 gagtacctgt cgcgcgaggc cggccttccg cccggcgccg cgatggccta cctggtcgcc
     1261 ccgccgatcg aggcgacgat cgggctcgac gccgcgctca aggccgccga cgtccgcgcc
     1321 gtgcgcgtcg tgccgccgcc gaccgagacc aacttcgcgt gcgcctggct gaccggctcg
     1381 ctcgacgcct gcgaggccgc cgcgctcgcc tacgccgccg ccgtcgtcga cgtcgcgctc
     1441 gcgccgaagc gatagcgcgc ctgcgcacgg tgcggtgtcc gccgcggccg cgcgtcgccg
     1501 ctggcactcc actcgcaggc ccatgacctt cgaaggagaa tccacatggc gaccgagacg
     1561 aaacggaaat ttgctgtggt gaccggggcg tcgtcgggga tcgggttcga gctcgcccgg
     1621 cagtgcctgg agcacgggtt cgacgtcctg atctgcgccg aggacgccgc gatcgacggc
     1681 gcggccaagc agctcgagac cgccggtgcg ctcgtcatcc cgttccaggc cgacctcgcg
     1741 acctacgacg gcgtcgagcg gctgtaccag gcgatccgcg accagcaccg cccggtcgac
     1801 gcgctgctgc tcaacgccgg catcggcgtc ggcggtgagt tcatcaagac cgacctcgcg
     1861 gccgacctcc ggacgatcgc gctcaactgc aaccacaccg tccacctcgc caagcgcgtg
     1921 ctgccagaca tggtctcgcg ccgccagggc cgggtgctga tgaccggctc gatcgtcagc
     1981 acctcaccca acccctacca ggcggtgtac ggcgcgacca aggccttcgt gatgtcgttc
     2041 ggtgaggcgc tgcgctacga gctcaaggac accggcgtca ccgtcaccgt gctccagccc
     2101 ggcgtgaccg atacccggtt cttcgagcgc gccgacctgc gcgacaccaa gatcggccag
     2161 tccagcaagg acgatccggc gctggtcgcc cggcaaggct tcgacgccat gatggccggc
     2221 aaggactcgg tgctcgccgg ctcgctcaag agcaaggtcg agggcctgat gaacgagcta
     2281 ttgcccgaga cggtcaaggc cgcccaggcc gccaagcgca cccggcccgg ctcggccaag
     2341 cagtgatgct cacccgcgcg acacaccggg agcagatagg cgagccatgg ctgagccgtt
     2401 gcgcgcggcc gtgatcggct cggggttcgg cgggctcgcg gccgcgatcc ggctggccgc
     2461 gatgggcgtg cgggtcacgc tgttcgagg
//
LOCUS       DASVOR010001562        16031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_337147,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001562 DASVOR010000000
VERSION     DASVOR010001562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 16031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16031
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337147"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="VF516_29345"
     CDS             complement(<1..1002)
                     /locus_tag="VF516_29345"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEX8111887.1"
                     /translation="MSLQEVSNSVRFADLELEVLARWERERTFQRSVERRAGAPRFVF
                     YDGPPFASGLPHYGHILTSFIKDVVPRYQTMRGYHVPRRWGWDCHGLPVELEAERALG
                     LTSPAAIAEHGIGPFNATCRALVTRYTAEWQQVMARLGRWVDFERAYRTMDPGYIESV
                     VWCFHALHQRGLVYEGEKVVPYCTRCQTALSNFETRLDDAYRPRVDTAATVAFALAPH
                     EAVLAWTTTPWTLPANAALAVHPELAYTRLERGGDSVWLADAARERYAAELAGYTVRE
                     TVPGRELVGRRYRPPLDAWSGAFPGERRVVAADWVSAGDGTGAVHLAPAFGEDDQTLC
                     "
     gene            complement(1291..1518)
                     /locus_tag="VF516_29350"
     CDS             complement(1291..1518)
                     /locus_tag="VF516_29350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111888.1"
                     /translation="MQQILDAHLAPAFAMRLTAADRTRALRIAVGRPGVLVALVRTLT
                     QARYWRAGRPALDLAASDLAIEQVSSRRHPP"
     gene            1495..2121
                     /locus_tag="VF516_29355"
     CDS             1495..2121
                     /locus_tag="VF516_29355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111889.1"
                     /translation="MRIEDLLHRIAVERWDRDLAMGQAERAQACMLARRVRSSRKDRP
                     DPDRPELSNLATHHGTTPLDAVDEQCGWIMLDAAPELRLRCSRRLGVRPRERLHQIRI
                     AREALDPDRAVADGSEPCFAGVEQRRCTDATRTVDQHGVAGERVRGQCLDEGSASHDL
                     HDDSFSERKQSSAALRDGHGELHRQLDLSRARYPFRPREASRTSGHLR"
     gene            2178..3707
                     /locus_tag="VF516_29360"
     CDS             2178..3707
                     /locus_tag="VF516_29360"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111890.1"
                     /translation="MGFRSVLFRGSEDPAPPPEPPACFRDLHLDQVVQAIAAGSRAHD
                     VASLYWLHLADADAIAYRQEVARDLDRDDVRRAVTAFVDAMQAMRERLELAGKLHYYR
                     YETTRWFLHAVEIYVDAVEALARDLEPAAPASRGLRGLLAYVVGYAASARFTELATTA
                     RRLASDLAAIRYCMVILPGSVTVRPDQGERDYSADVETTFAKFRRTAPKDYRVKLPHR
                     VGIDHVEAAVLERVAQLHPDAFRALEAFRAEHAEYLDPTIARFDREVQFYLAYLAYIA
                     RPRRAGLPFCFPELAEDKRVHARGAFDLALATRLVERNATAVPNDFHLQSSERVFVVS
                     GPNQGGKTTFARMVGQLHYLASLGLPVPGREARLALFDELFTHFEREEDPDNQRSKLH
                     DDLLRIHAILAAATPRSLAIINEMFSSTTLADAVLLGRRVMARISSLGMLAVWVTFLD
                     EISSFDARTVSLVSTVDPQDPAIRTFRLVRAPPSGVAYALTIAEKHGVTYERLKERIR
                     S"
     gene            3704..5206
                     /locus_tag="VF516_29365"
     CDS             3704..5206
                     /locus_tag="VF516_29365"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111891.1"
                     /translation="MTPRLLSRERDLDLAQVLPAHAQALTQDLGIDLLLHAMAGEDPF
                     LRDVARKVVLSAVIGDVDAIRYRQAILVDAMRAPEVTRELYGVAVEALEARRKHWFFG
                     LSSKYPSTTLSSGVEHLQGSVGFLRKLRNIADTQRGRFTSEGFGNLFAMLQAELSDAY
                     FAEVEQHLRELQFREGVMISAALGPGNAGADFVLRRPHGRSTWWRRMLGKGPPGLTFR
                     IDERDDAGARALGELRDRGINLVANAVAQAAAHVESFFTLLRAELAFYVGCLNLRDRL
                     MAIGSPICIPDPAPAGAHAYRFTELYDPGLALAARRPPVGNALAADRAGLVMITGANQ
                     GGKSTLLRALGLAQLMMHAGLFVAAEAFTANVCHGGVFTHFKREEDVAMKSGKLDEEL
                     RRISELADALAPDALVLFNESFASTNEREGSEIARQVVSALLERRVKVVFVTHLYDFA
                     RSMFERRLDGTVYLRADRESDGARTFKLVPGEPLTTSFGTDLYRDVFGDT"
     gene            complement(5421..6590)
                     /locus_tag="VF516_29370"
     CDS             complement(5421..6590)
                     /locus_tag="VF516_29370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111892.1"
                     /translation="MREAALVVLGPLALSSIAAADPAPAVEPAASPASAAAPASAPET
                     PPPAAASSQPAPAAAREEPIWRPDQYFYAEGFLITTHFFPMDPAAAAPDLERVLVRGM
                     WAWAVPLHVTASGLQLTGTSLVEASVLEANGSWQSFDQNRLCLGLAVTGRRFNVWNQV
                     GRCYSPAKDPYALEGPDPMVGFASVYWAYRTDNPFRQYGWRTDVNLQPGATRLHLSAM
                     LAVDEAALTARGHERFALDRAIGAFVWRFPNDCRALGMINVGFETAANNYGGVHAECA
                     LWGAWEWGVGGLVGGPHNENGHGLQVLNRLSRTMPDRQNMELVGTATISFLRWPGAHG
                     KMYSLGGEGTWRFANGLGMTAGAFQVWLDRAALVEGGRRGMTETEAFMNLSWLYE"
     gene            complement(6626..7528)
                     /locus_tag="VF516_29375"
     CDS             complement(6626..7528)
                     /locus_tag="VF516_29375"
                     /inference="COORDINATES: protein motif:HMM:NF017807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8111893.1"
                     /translation="MLRYYGLLLLSASAVASAPILVRVSEVGPTATLWLRMGVALLIL
                     LTPIGGHDDAAAAEPTVGHRPGPARLLSGIVLSGFLFCADMLANHWAVRMTSVANTTL
                     LMNLTPVFVIAIAYVFLRERQSLISVAGVIAASAGAAVLVGASYSLGRDHLIGDALAL
                     SSALLYAIFMVVTKALRSWVSATRLLRWHTGLSCVFLLPLVAGGHAQVFPVTAQGWAI
                     VVGLAVISQLLGHGLMTYAVKHVSAGISSMSTLIIPVLSTAMAWLLLGEPIAPHHLLG
                     GGLVRIGIGLYAVHDLRVEKGQPA"
     gene            complement(7528..8808)
                     /locus_tag="VF516_29380"
     CDS             complement(7528..8808)
                     /locus_tag="VF516_29380"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8111894.1"
                     /translation="MKPMVVVLEPVSSGVSLLEAARRLGYATIAVSHDADDRTLSADA
                     RSAADEVVRCDTNDLSALTETVQRLAQRHTVKAVLPGFEYYVTAATRINAQLKLPGLP
                     LDSADCVRNKHAMRTRLASRGVRVPRFAFVRTQAELLEAARIIGYPAVLKPTEGSGSM
                     HVRRVDDQGQLLDAFQHIQADASTDLGRSFHDRLLLEEYITGPEISVEGFISTTGSTT
                     GVEVVSITQKLLGPEPWFVELGHIVEGEFDAATRRQITAYVREVTAALDIRLGTFHCE
                     LRLSRGGPVVIEIGARLAGDRICRLIELAKGIALPDVLVRAYTGEPIAALPVVDRCHA
                     GITFLSAPPELAVFRAFDGLDTLRDAVDGVEEIVAHAHPGDPLPPLTDFRARLGHVIV
                     TAPSYLELTRRLAAVKQHIVVRGDRGADGRPEAR"
     gene            complement(9044..9208)
                     /locus_tag="VF516_29385"
     CDS             complement(9044..9208)
                     /locus_tag="VF516_29385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111895.1"
                     /translation="MSLVHRSADRAASGVEALHSVPFPMMVRVDRRDAKRLHRRVASM
                     QRRVSLRPGR"
     gene            9759..10454
                     /locus_tag="VF516_29390"
     CDS             9759..10454
                     /locus_tag="VF516_29390"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8111896.1"
                     /translation="MIYQFTVEQAKNHATAQMEKLFATVPFARDFHAGAASHPEYYKR
                     HLMETILRIRLNNEVDSYCLYKIGYKNNKLAVKLAKYLAEEFGHENFFLQDLKGLGVE
                     KETLDATPPFFSTEKLIGYLYHAINQDGPMPTMVWNWFVEWYSDTYNKIITEKATNVV
                     GEKGTKGFKKHIEFDEDHDHISLMFGTVEQTVNSQADHQKVKNYLTNFIALIGEYFQE
                     LYDSTIGMKSKAE"
     gene            10526..11755
                     /locus_tag="VF516_29395"
     CDS             10526..11755
                     /locus_tag="VF516_29395"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8111897.1"
                     /translation="MRRTKLIIIEPSSSGVALIPAASSLGFDPVVATYNRDDRVVPRE
                     LLALASDVIEVNTIDAAALSEAIHRHARGHQIAGVVPGFEYYVGLAARLSHALGLRGL
                     DPAIVDNLRYKDKMRDALSAHGVACPRYLTVTDRSMLAQVEARVGYPCVIKPVNGSGS
                     LHVTKVHDRAELEAAYDGIGQDDTIDMGLRMSQVAVVESYVVGPEFSVEGYVHDGRIH
                     FLSVTEKMLCQEPHFAEVGHIVNTCMRDEERAAIYGYTASVVRALQIGLGPFHCELRV
                     SAGQPIAMEIAARLPGDSIVELIHESTGVNLAEVMIRSYVGMDVPPPPAVAARYAGVC
                     FFTLDNANYFHAVDGVSAVKQLPGFKRFELLAQPASYVPPPISSLGRAAKAIFAGPSY
                     HDLKLEMEQAKSLIRFH"
     gene            11783..13021
                     /locus_tag="VF516_29400"
     CDS             11783..13021
                     /locus_tag="VF516_29400"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8111898.1"
                     /translation="MPETILIINRWDDDFSRYDEYIDHRVHRVAYVTNPAGSQIVRRE
                     LACGLEIVPDITDRRAVIAAATRLAGAVGPFTRVLALSEFDLVTGALVREALGTPGAM
                     PQQVRRFTDKVVMKQAILAAGLRAPRYLPLVEAPTAEALIDRLGLPLILKPRVGASSR
                     GVSKVETAEQLATALRDIDPADYECEQYIAGDIYHVDGLVRDGELLFHSVSRYINTCL
                     AFNDGVPLGSVLLEPSARRTALVAFTDAALRALELRSGAFHLEVIAGTGVGPGDELYF
                     LEIGARVGGGEIPFLGQALFELDMMRAWVDVELGHDTEYLRAIDDNRLGGFLMIPEPR
                     DVPASLVRVSSLAGKVDCLLKETLPPVGHVFDGKGGYEHISGRFLFAGGSSQEVETAI
                     QSAIAQFSIETTRIRSAATG"
     gene            complement(13056..>16031)
                     /locus_tag="VF516_29405"
     CDS             complement(13056..>16031)
                     /locus_tag="VF516_29405"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8111899.1"
                     /translation="APAPTDAPPPAPPAPATPPDAAGPATPPAPADAPLPAEVVPPTE
                     GEVIMVTGSRIGDPLGKQAPVLMMSREDLDRTGLTSVGDILQQLPVSGGAINGKFNSS
                     GNFGFPPDGGGIGAGAVEADLRYLGSKRVLVLVDGVRWVNGSSGSGVAAATDLNTIPL
                     SIVERIEVLEDGASPIYGSDAIAGVINIITRKEFSGAVGSIYTGGFNHGDGFVQKYDV
                     SWGSTSANGKTSTVVGASFLDQGAVSSADRTISQFPIPAIGHCTSKCSSATPQGREIF
                     HDPNTDQDQNITLNNGVGTPVYPTDYHDFTTADRFNFSPYNYVETPSRRMGAYSSLTY
                     KLLSNVNLRGQAAFTNRVSANQAAPEPLFIGPEGGNGNRLDTIKIDATNPYNPFGFTL
                     DPATNDYVVTRRPVEAGPRHFEQDVNTFYVSGGANGHFDVDTQRFNWDATVAYGLNRA
                     DQRRSNAFNSAKLQEALGPAYLGSDGRYHCGTMANPGSPNCVPFNIFGGQGADGKGTI
                     TKDMLAFTTFTEHDVSEQTLVDAVANLSGNLVKLPAGWLAGAVGVEVRRLSGFYEPDG
                     IVSAGDGADVPSQPTSGKYSVGEAYAELRAPLVIGKPGAELIDVNAAARLSDYSFLNS
                     VKLTGKIGARWKPTTDLVIRGSFGQGFRAPSIGELYGSKARFDAVLVDPCSGFNDPKK
                     PVPPEVKQRCIALGVPADGGYTQLNQQISVTTGGNLALNPETSKSFNLSVAYSPKQLQ
                     DRPWVDSFDAELAYWDIRLDNAISALDAQYQIDRCVGGMVDAYCMGIQRNNAGTIFSF
                     GNQLQNIGGINTRGLDLSLVYRAPRKPFGRFRVSSQSSYLLAYEERVPAGAGFETVDR
                     TGTIFGAPERAFPRFKSALAIGWLYGQFDATLTTRYIHSLTEQCRDLGDFPGTCSNPN
                     STDDSLSTNKLGIAVYNDLQLVWSPEFDRGLTVTAGVNNLFDRDPPTCFSCSLNGFNP
                     ATYDVPGIFGYLSATYHMQ"
BASE COUNT         2595 a         5434 c         5535 g         2467 t
ORIGIN      
        1 gcacagggtc tggtcgtcct cgccgaacgc cggtgcgagg tgaacggcgc cggtgccgtc
       61 gccggcgctg acccagtcgg cggcgaccac gcggcgctcg ccggggaagg ctccggacca
      121 tgcatcgagc ggcggccggt agcgccggcc gaccagctcg cggccgggca cggtctcgcg
      181 caccgtgtac ccggcgagct cggcggcgta gcgctcgcgc gcggcgtccg cgagccacac
      241 gctgtcgccg ccgcgctcga gccgggtgta ggccagctcg ggatggaccg cgagcgcggc
      301 gttggccggc agcgtccacg gcgtcgtggt ccacgccagc accgcctcgt gcggcgccag
      361 cgcgaacgcg accgtggccg cggtgtcgac ccgcgggcgg taggcgtcgt cgagccgggt
      421 ctcgaagttc gacagcgcgg tctggcaccg cgtgcagtac ggcacgacct tctcgccctc
      481 gtagaccagg ccgcgctggt ggagcgcgtg gaagcaccac accaccgact cgatgtagcc
      541 gggatccatc gtgcgatacg cgcgctcgaa gtcgacccag cgcccgagcc gcgccatcac
      601 ctgctgccac tcggcggtgt agcgggtgac cagcgcgcgg caggtcgcgt tgaacgggcc
      661 gatgccgtgc tcggcgatcg ccgccggcga ggtcagcccg agcgcgcgct cggcctcgag
      721 ctcgaccggc aggccgtggc aatcccagcc ccagcgccgc ggcacgtggt agccgcgcat
      781 cgtctggtag cgcggcacca cgtccttgat gaacgaggtc aggatgtgac cgtagtgcgg
      841 cagcccgctc gcgaacggcg ggccgtcgta gaacacgaag cgcggcgcgc cggcgcggcg
      901 ctcgaccgat cgctggaacg tgcgctcgcg ctcccagcgc gcgagcacct cgagctcgag
      961 atcggcaaac cggacggaat tcgagacttc ctggagcgac atgcgagata cgccttctgc
     1021 cccggcagcc gggggcggtg acgacagacc tgagcgaacg gacggtcagt cgcgcggcgt
     1081 aatcgagcgg ccgtcgaagt gagcccggcc gcgagcttcg ctcgcagctt ccacagcagg
     1141 tggctggact ctcgacggca cagacgctcc cggttcctcg gactatagcg aaccgccggc
     1201 gcggcgcaag cctcgcggcg agtgcgccgg gatgcacgac ggctcgcggc actttgctcg
     1261 acgatgatag gcaggaccac cgcgatcggc tcatggtgga tgcctccggc tggacacctg
     1321 ctcgatcgca agatcggagg cggcgaggtc cagcgcggga cggcccgcac gccagtacct
     1381 cgcttgtgtc agtgtgcgta cgagcgccac gagcacgccc ggccgcccga ccgcgatgcg
     1441 cagagcacga gtgcgatccg ccgcggtcag ccgcatcgcg aaagccggag ccagatgcgc
     1501 atcgaggatc tgctgcatcg catcgcggtc gagcggtggg atcgcgatct cgcgatgggc
     1561 caggcggagc gcgcgcaggc gtgcatgctc gcgcggcgag tccgctcgtc ccgcaaagat
     1621 cgccccgacc ccgatcgccc cgagctctcg aaccttgcga cgcaccatgg caccaccccg
     1681 ctcgacgcgg tcgacgagca atgcggctgg atcatgctcg atgcggctcc ggagctgcgc
     1741 cttcgatgtt cgcgtcgcct cggtgtacgc ccgcgcgagc ggctccatca gatccgcata
     1801 gctcgcgagg cgctcgatcc ggatcgagcg gtggcggacg gttccgagcc gtgcttcgca
     1861 ggcgtcgagc agcgtcgatg caccgatgcc acgcggaccg tagaccagca cggcgtggcc
     1921 ggcgagcgcg tgcgcggtca gtgtctcgac gagggcagcg cgtcccatga tctgcatgac
     1981 gactccttca gtgaaagaaa gcagtcatcg gccgctttgc gcgacggtca cggcgaactc
     2041 caccgccagc tcgatctatc tcgagcacgc taccccttcc gcccgcggga ggccagccga
     2101 acgtccggtc acttgaggtg aagggtgaac ggcgcgaaga tcaagcccag ctcctgaagg
     2161 ttcgcaggag gatcgccatg gggtttcgca gcgtgctgtt tcgaggatcg gaggacccgg
     2221 cgccgccgcc ggaaccaccg gcatgcttcc gcgatctcca tctcgaccag gtcgtgcagg
     2281 cgatcgccgc aggctcgcgc gcacacgacg tcgcgtcgtt gtactggctg cacctggcgg
     2341 acgccgacgc catcgcctat cggcaggagg ttgcacgtga cctcgatcgc gacgacgtga
     2401 ggcgcgccgt gaccgcgttc gtggatgcca tgcaggccat gcgcgagcgc ctcgagctcg
     2461 cgggcaagct ccactactac cggtacgaga ccacgcgctg gttcctccac gcggtggaga
     2521 tctacgtcga tgccgtcgaa gcgctggcgc gcgatctgga gccggctgca cctgcctcgc
     2581 gcgggctgcg cggactgctc gcatacgtgg tcggctacgc agcgtcggcg cgattcaccg
     2641 aactggcgac cacagcgagg cgcctcgcgt ccgatctcgc cgcgatccgc tactgcatgg
     2701 tcatcttgcc cgggagcgtc acggtgcgac ccgaccaggg cgagcgcgac tacagcgctg
     2761 atgtggaaac aaccttcgcg aagttccgac gtacggcccc caaggactac cgcgtcaagc
     2821 tcccccaccg cgtgggcatc gatcatgtcg aggccgcggt gctggagcgg gttgcgcagc
     2881 tccatcccga cgcgttccgc gcgctcgaag cattccgcgc cgagcacgcc gagtacctgg
     2941 accccacgat cgcgcggttc gatcgcgagg tgcagttcta cctcgcctac ctggcctaca
     3001 tcgccaggcc aagacgcgca ggcttgccgt tctgtttccc ggagctcgcc gaggacaaga
     3061 gggtccatgc gcgcggtgcg ttcgatctcg cgctcgccac caggctcgtc gagcgtaacg
     3121 ccaccgcggt gcccaacgac tttcacctgc agagcagcga acgggtgttc gtggtgtccg
     3181 gtcccaacca gggcgggaag acgaccttcg cgcggatggt cggtcagctc cactacctgg
     3241 cgagcctggg cttgccggtc ccgggacgcg aggcacggct cgccctgttc gacgagctgt
     3301 tcacccattt cgagcgagaa gaagacccgg acaaccagcg cagcaagctg cacgatgatc
     3361 tgctccggat ccacgcgatc ctcgccgcgg cgacaccgcg cagcctcgcg atcatcaacg
     3421 agatgttctc atccacgacg ctcgccgatg ccgtgctgct cggccgccgc gtgatggcga
     3481 ggatctcgag cctgggcatg ctggcggtgt gggtcacgtt cctggacgag atctcgtcgt
     3541 ttgacgccag gacggtcagc ctggtcagca cggtggatcc gcaagatccg gcgatccgca
     3601 cgttccggct cgtccgcgcg ccaccgagcg gcgtcgcgta tgccttgacg atcgccgaga
     3661 agcacggcgt gacgtacgag cggttgaagg agcggatccg gtcgtgacgc cgcgcctgct
     3721 gtctcgcgag cgcgacctcg atctggcgca ggtacttcct gcacacgccc aggcgctcac
     3781 acaagatctc gggatcgacc tcctgctgca cgcgatggcc ggcgaggacc cgttcctgcg
     3841 cgacgttgcc cgcaaggtgg tcctgtcggc cgtgatcggc gatgtcgatg cgatccgcta
     3901 ccgccaggcg atcctcgtcg acgcgatgcg tgcgccggag gtcacacgcg agctctacgg
     3961 cgttgccgtc gaggcgctcg aggcgaggcg caagcactgg ttcttcgggc tgtccagcaa
     4021 gtacccgagc accaccctgt cgagcggggt cgagcacctg caggggtcgg tcggctttct
     4081 ccgcaagctg cggaacatcg ccgacacaca gcgcggccgt ttcacttccg agggcttcgg
     4141 aaacctgttc gcgatgctgc aggcggagct gtctgacgcg tacttcgccg aggtagagca
     4201 gcacctgcgc gagctgcagt tccgcgaggg cgtgatgatc agcgccgcgc tgggtccggg
     4261 caatgcgggg gcggacttcg tcctgcgcag gccgcacggc cggagcacct ggtggcgacg
     4321 catgctcggc aagggaccac ccgggctgac gttccggatc gacgagcggg acgatgccgg
     4381 cgctcgggcg ctcggcgagc tgcgcgaccg cgggatcaac ctcgtggcga acgcggtcgc
     4441 acaggccgcc gcccacgtcg agagcttctt cacgttgctc cgcgccgagc tggcgttcta
     4501 cgtcggatgc ctgaacctgc gcgaccggct catggcgatc ggctcgccga tctgcatccc
     4561 ggatccagcg cctgccggtg cacatgccta ccgcttcacg gagctctacg atccaggcct
     4621 ggcgctcgcc gctcggcgcc cacccgtggg caacgcgctc gcggcggatc gcgcgggcct
     4681 ggtgatgatc accggggcga accagggcgg caagtcgacg ctcctgcgcg cgctggggct
     4741 cgctcagctg atgatgcacg ccggcttgtt cgtcgcggcc gaggcgttca cggccaacgt
     4801 ctgccacggc ggtgtgttca cgcacttcaa gcgggaggag gatgtcgcga tgaagagcgg
     4861 caagctcgac gaggagctgc gccggatcag cgagctggcc gacgcgctgg ccccggacgc
     4921 gctcgtcctg ttcaacgagt cattcgcctc gaccaacgag cgcgaaggct ccgagatcgc
     4981 gcgccaggtc gtctccgcgt tactcgagcg acgagtcaag gtcgtgttcg tcacacacct
     5041 gtacgacttc gcgcgcagca tgttcgagcg aaggctcgac ggcacggtgt acctgcgcgc
     5101 agaccgcgag agcgacggcg ctcgtacgtt caagctcgtt cccggcgagc cgctcaccac
     5161 cagcttcgga accgacctgt accgcgacgt gttcggcgac acatgacgcg gtccgtagat
     5221 ctcgaagtgg tttcgagctc gtcggaggtc aaccgagatc cggcggtctg gcgcatgtcg
     5281 gctatgaacg tcgcgacgag ccggtccgcg ttcttggcgg cgtcgagcgc cggccgccac
     5341 agcgacggct tgagcagccg caggtaccag gtcgacagcg cagtgatgaa gtcctcgagc
     5401 gccgcgtagc gcgccgcggc ctactcgtag agccaggaca ggttcatgaa cgcctcggtc
     5461 tcggtcatgc cgcgccgccc accctcgacc agcgcggcgc gatccagcca gacctggaac
     5521 gcgccggccg tcatgcccag gccgttggcg aaccgccagg tgccctcgcc gccgagcgaa
     5581 tacatcttgc cgtgcgcacc gggccagcgc aggaagctga tcgtcgcggt gccgaccagc
     5641 tccatgttct ggcggtcggg catcgtgcgg ctcagccggt tcaagacctg cagaccgtgc
     5701 ccgttctcgt tgtgcggccc gccgaccaac ccgccgacgc cccactccca cgcgccccac
     5761 agcgcgcact cggcgtggac cccgccgtag ttgttggccg cggtctcgaa gccgacgttg
     5821 atcatgccga gcgcgcggca gtcgttgggg aaccgccaca cgaacgcacc gatcgcgcga
     5881 tcgagcgcaa accgctcgtg tccgcgagcc gtgagcgccg cttcatcgac cgccagcatg
     5941 gcgctgaggt gcaggcgggt ggcgcccggc tgcaggttga cgtcggtgcg ccagccgtac
     6001 tgccggaacg gattgtcggt gcggtacgcc cagtagaccg atgcgaagcc caccatggga
     6061 tccggtcctt cgagcgcgta cgggtccttg gccggcgagt agcagcggcc gacctggttc
     6121 cacacgttga accgccggcc ggtcaccgcg aggcccaggc acaggcggtt ctgatcgaac
     6181 gactgccagc tgccgttggc ctccagcacg ctcgcctcga cgagcgatgt cccggtgagc
     6241 tggagcccgc tggcggtcac gtgcagtggc acggcccacg cccacatgcc gcgcaccagg
     6301 acgcgctcca gatccggcgc cgcggccgcc gggtccatgg ggaagaagtg ggtcgtgatc
     6361 agaaagccct cggcatagaa gtactgatcg gggcgccaga tcggctcctc gcgcgcggcc
     6421 gccggagccg gctggctcga ggccgctgcc ggtggagggg tctctggagc cgaggccgga
     6481 gccgcggccg acgctggcga cgccgcgggt tcgaccgccg gcgccggatc ggccgcggcg
     6541 atcgacgaca gcgccagcgg cccgagcacg acgagcgccg cctcgcgcac accacggcct
     6601 cggtgtgcgg cccgggcgcc cggcatcaag ccggctggcc tttctcgacc ctcaggtcgt
     6661 gcaccgcgta cagcccgatc ccgatccgga ccagcccgcc gccgagcagg tggtggggcg
     6721 cgatgggctc gccgagcagc agccatgcca tcgcggtcga cagcaccgga atgatcagcg
     6781 tggacatgga cgagatcccg gccgagacgt gcttgacggc atacgtcatg aggccgtggc
     6841 cgagcagctg gctgatcacc gccaggccga ccacgatcgc ccagccctgc gcggtcaccg
     6901 ggaacacctg ggcgtgaccg ccggccacca gcgggagcag gaacacgcag ctcagcccgg
     6961 tgtgccagcg cagcaggcgg gtcgccgata cccagctgcg cagcgccttg gtcacgacca
     7021 tgaagatcgc gtagagcagc gcggaggaca gcgccagcgc atcgccgatc aggtgatcgc
     7081 ggcccagcga gtagctggca ccgaccagga cggccgcccc cgcgctcgcg gcgatcaccc
     7141 cggccacgct gatcaacgat tgccgctcgc gcaggaacac atacgcaatg gcgatcacga
     7201 acaccggcgt caggttcatc agcagcgtgg tgttggccac cgaggtcatc cgcacggccc
     7261 agtgattggc gagcatgtcg gcgcagaaca ggaacccgct gagcacgatg ccggacagca
     7321 gtctcgccgg accgggcctg tggccgaccg tcggctcggc cgccgcggca tcgtcatggc
     7381 cgccgatcgg ggtgagcaag atcagcagcg cgacgcccat tcgcagccag agcgtggcgg
     7441 tgggcccgac ctcgctgacc cggaccagga tcggagccga cgcgaccgcc gacgcgctca
     7501 gcagcaagag cccgtagtag cgcagcatca gcgcgcttcc gggcgcccgt cggcgccgcg
     7561 atcgccgcgg accacgatgt gctgcttgac cgccgcgagg cggcgggtga gctcgaggta
     7621 gctcggcgcg gtgacgatca catggcccag ccgggcgcgg aagtcggtga gcggcggcag
     7681 gggatcgccg gggtgggcgt gtgcgacgat ctcctcgacg ccgtcgaccg cgtcgcgcag
     7741 ggtgtcgagg ccgtcgaacg cgcggaacac cgcgagctcg ggcggcgccg acagaaacgt
     7801 gatcccggcg tgacagcggt cgacgaccgg cagcgcggcg atcggctcgc cggtgtacgc
     7861 ccgcaccagg acgtcgggca gcgcgatgcc cttggccagc tcgatcaagc ggcagatccg
     7921 gtcgccggcg agccgggcgc cgatctcgat cacgaccggt ccgccgcgcg acagccgcag
     7981 ctcgcaatgg aacgtgccca gcctgatgtc gagcgcagcc gtcacctccc gcacatacgc
     8041 ggtgatctgc cgccgggtcg cggcatcgaa ctcgccctcg acgatgtgcc cgagctcgac
     8101 gaaccacggt tcgggcccga gcagcttctg cgtgatcgag acgacctcga cgccggtggt
     8161 gctgccggtg gtgctgataa acccctcgac gctgatctcg gggccggtga tgtactcctc
     8221 gagcaggagc cggtcgtgga agctgcgccc cagatcggtg ctggcgtcgg cctggatgtg
     8281 ctggaaggca tcgagtagct gcccctgatc gtcgactcgc cgcacgtgca tgctccccga
     8341 accctcggtc ggcttgagca ccgcagggta gccgatgatg cgcgccgcct ccagcagctc
     8401 ggcctgggtc cggacgaacg cgaacctggg gacccggacg cctcggctgg cgagccgggt
     8461 gcgcattgcg tgcttgttgc gcacacagtc cgcgctgtcg agcgggagtc cggggagctt
     8521 cagctgagcg ttgatgcggg tcgccgccgt gacgtagtac tcgaagccgg ggaggactgc
     8581 cttcacggtg tggcgctgcg cgaggcgctg cacggtttca gtcagcgcgg acaggtcgtt
     8641 ggtatcgcag cgcaccacct cgtcagccgc cgagcgcgcg tcggcagaca gcgtccgatc
     8701 atcggcgtca tgcgacactg cgatcgttgc gtacccgagc cggcgggccg cttcgagcag
     8761 gctcacgccg gaggacaccg gctccaggac gactaccatc ggcttcatac acacgattcc
     8821 tcgatctcgg cggcatgacc gcttcgatcc tcacggacaa gaaatgggga gactgattcc
     8881 catggaggcg ccgccacggc catcctccgg ggcaagctca tgtagcaagg cggttatacg
     8941 ccgtcaatag cgcctggccg cgccgcgagt ccaaaaggac gacgtgatgg agttcatcac
     9001 agcgcggacg aacgcggaat gtgcgagatc cgcgctccgc ctgtcaccgg ccgggccgca
     9061 gcgaaacgcg gcgctgcatc gacgcgacgc ggcgatgcag ccgcttggca tcacggcgat
     9121 cgacgcgaac catcatcgga aatggaaccg aatgcagcgc ttcgacaccg gatgccgcgc
     9181 ggtccgcgga gcggtggacc aggctcacgc cgggcgatcc gcgtgggacc acgctccggc
     9241 tctacctgtc agcgctgtac gccgccatac ggaccgtcct gcatttgcgc ctcacgtacg
     9301 cggctgcagc ggtcacgtcg cgcgtcggcc tcgatcggtg tcgcgcctgc ggcagggacg
     9361 ggacaagcgc ctgcagcgac agtcccgcct gcgacctgtg cgacaagcgc gtcgagacga
     9421 ggacgcgcca ttcttgattg gggaacaccg ctgtgcaagc gctgtaacag ctgagcgtga
     9481 tctcgattgc gatcggatgg cggtttcgac actccccatg ccgacgagat ccaacgcgca
     9541 tctccccacg acatctggcg attcacgtac catgtgttgc ctcgcgtaca cggccccatt
     9601 gcggtgcgtt acccgcctta ccagttgatc agtcgcactg aagcaaaatg atgcacatag
     9661 aatatcactt gcaatcttat ccgacatacc tatgctctgc ggacttacga atcgggtgga
     9721 ttcgacacgc caccgtctag ccgctacagg agatgaggat gatttaccag ttcaccgtcg
     9781 aacaagccaa gaaccacgcg accgctcaga tggagaagct gttcgcgacc gtcccgtttg
     9841 cgagggactt ccatgccggc gctgcgagcc atcccgagta ctacaagcgc cacctgatgg
     9901 agaccatcct ccggatccga ctcaacaacg aggtggattc ctactgtctc tacaagatcg
     9961 ggtacaagaa caacaagctg gcggtcaagc tcgccaagta cctcgccgag gagttcgggc
    10021 acgagaactt cttcctgcag gacctcaagg ggctcggcgt cgagaaggag accctggacg
    10081 cgacgcctcc gttcttctcg accgagaagc tgatcggcta cctgtaccac gcgatcaacc
    10141 aggacggtcc gatgccgacg atggtgtgga actggttcgt cgagtggtac tcggatacct
    10201 acaacaagat catcaccgag aaggcgacca acgtggtcgg cgagaagggc acgaagggct
    10261 tcaagaagca catcgagttc gacgaggatc acgaccacat cagcctgatg ttcggcacgg
    10321 tcgagcagac ggtgaactcg caggccgatc accagaaggt caagaactat ctgaccaact
    10381 tcatcgcgct gattggtgag tacttccagg agctgtacga ttcgacgatc ggcatgaagt
    10441 cgaaagccga gtaacggctg cacgcggctc gcgcgcatgg gggtgcgcgg cgagcccggt
    10501 cgccaccgcg ccacgaaggc aatccatgcg cagaaccaag ctgatcatca tcgaacccag
    10561 ctcttcgggg gtcgctttga tccccgcggc atccagcctg ggcttcgatc cggtcgtcgc
    10621 cacgtacaac cgggacgacc gcgtcgtgcc gcgcgagctg ctcgcgctgg cgtccgacgt
    10681 gatcgaggtc aataccatcg acgcggcggc gctgtccgag gcgatccatc gccacgcgcg
    10741 cggccaccag atcgccggcg tcgtccctgg cttcgagtac tacgtcgggc tggcggcgcg
    10801 gctcagccac gcgctcggac tgcgcggcct cgatcccgcg atcgtcgaca acctccggta
    10861 caaggacaag atgcgcgatg cgctgagcgc acacggcgtg gcgtgcccgc gctacctgac
    10921 ggtgaccgac cgatcgatgc tcgcacaggt cgaggcccgg gtcggctacc cgtgcgtgat
    10981 caagccggtc aacggctcgg gcagcctgca cgtcaccaag gtgcacgacc gggccgagct
    11041 cgaggctgcc tacgacggga tcggacagga tgacacgatc gacatgggac tgcggatgag
    11101 ccaggtcgcg gtcgtcgaga gctacgtcgt cggccccgag ttcagcgtcg agggctacgt
    11161 ccatgacggc cggatccatt tcctgtcggt gaccgagaag atgctgtgcc aggagccgca
    11221 ctttgccgag gtcgggcaca tcgtcaacac ctgcatgcgc gacgaggagc gcgcggcgat
    11281 ctacggctac accgcgtcgg tcgtgcgcgc gctgcagatc gggctcggac cgttccactg
    11341 cgagctcagg gtcagtgcgg gccagccgat cgcgatggag atcgccgccc ggctgccggg
    11401 cgacagcatc gtcgagctga tccacgagtc gaccggcgtg aacctcgccg aggtgatgat
    11461 ccgcagctac gtcggcatgg acgtgccccc gccccccgcc gtggccgcgc gttacgccgg
    11521 cgtctgcttc ttcacgctcg acaacgccaa ctacttccac gcggtcgacg gcgtgtcggc
    11581 ggtcaagcag ctgcctgggt tcaagcgctt cgagctgctc gcccagccgg cttcgtatgt
    11641 gccgccgccg atcagctccc tcggccgcgc ggccaaggcg atcttcgccg gaccgtcgta
    11701 tcacgatctc aagctggaga tggaacaagc caagagcttg atccgatttc actgaccctg
    11761 ccgaacattg gcgaggtgtg ccatgcctga aacgatcctg atcatcaatc gctgggacga
    11821 cgacttctcg cgctacgacg agtacatcga tcatcgcgtc caccgcgtgg cctacgtgac
    11881 gaaccccgcg ggcagccaga tcgtgcgccg agagctggcg tgcggcctcg agatcgtccc
    11941 cgacatcacc gatcgccgcg cggtgatcgc tgcggcgacg cggctggcgg gcgcggtcgg
    12001 cccgttcacc agggtgctcg cgctgtcgga gttcgatctg gtcaccggcg cgctggtccg
    12061 cgaggcactg ggtacgccgg gcgccatgcc gcaacaggtc cggcggttca ccgacaaggt
    12121 cgtcatgaag caggcgatcc tcgccgccgg gctgcgcgcg ccgcgctacc tgccgctggt
    12181 cgaggccccg accgccgagg cgctgatcga ccggctcggc ctgccgctga tcctcaagcc
    12241 gcgggtcggc gcgtcgagcc gcggtgtcag caaggtcgag accgcggagc agctggcgac
    12301 ggcgctgcgc gacatcgacc cggcggacta cgagtgcgag cagtacatcg cgggcgacat
    12361 ctaccacgtc gacggcctgg tccgcgacgg cgagctgctg ttccacagcg tgtcgcgcta
    12421 catcaacacg tgcctggcat tcaacgacgg cgtcccgctc ggctcggtgc tgctggaacc
    12481 gtccgcgcgt cgcaccgccc tggtcgcgtt caccgatgcg gcgctccgcg cgttggagct
    12541 caggtccggc gcattccacc tcgaggtcat cgccggcacg ggggtcggcc cgggcgatga
    12601 gctctacttc ctggagatcg gcgcgcgggt cggtggcggc gagatcccgt tcctcggaca
    12661 ggcgctgttc gagctcgata tgatgcgggc atgggtggac gtcgagctcg gccacgacac
    12721 ggagtacctg cgggcgatcg acgacaaccg cctcggcggg ttcttgatga tccccgagcc
    12781 acgcgatgtc ccggcgagcc tggtccgcgt gagctcgctc gccggcaagg tcgactgcct
    12841 gctcaaggag acgctgccac cggtcggcca cgtgttcgac ggcaagggcg gatacgagca
    12901 catcagcgga cggttcctgt tcgcgggcgg ctcgtcgcag gaggtcgaga ccgcgatcca
    12961 gagcgcgatc gcgcagttct cgatcgagac cacccggatc cgcagcgcag ccaccgggtg
    13021 acgcgcctcg gatccgaaga cctcgcagcg ggccgctact gcatgtggta ggtcgcgctc
    13081 aggtagccga agattcccgg cacgtcgtac gtggccgggt tgaagccgtt gagcgagcag
    13141 ctgaagcagg tcggcggatc gcggtcgaac aggttgttga cgccggccgt gacggtgagc
    13201 ccgcgatcga actcgggcga ccacaccagc tgcaggtcgt tgtacaccgc gatcccgagc
    13261 ttgttggtcg acagcgaatc gtcggtcgag ttggggttcg agcacgtgcc cgggaagtcg
    13321 ccgagatcgc ggcactgctc ggtcagcgag tggatgtagc gcgtggtcag cgtggcatcg
    13381 aactgtccgt acagccagcc gatggcgagc gccgacttga accgcgggaa tgcgcgctcc
    13441 ggggcgccga agatggtccc ggtgcgatcg acggtctcga acccggcgcc ggcggggacg
    13501 cgctcctcgt aggccagcag atagctcgac tgcgaggaca cgcggaaccg gccgaacggc
    13561 ttgcgcggcg cccggtagac cagcgacagg tcgaggccgc gggtgttgat gccgccgatg
    13621 ttctgcagct ggttgccgaa cgagaagatc gtgccggcgt tgttgcgctg gatccccatg
    13681 cagtaggcgt cgaccatgcc gccgacgcag cggtcgatct gatactgcgc gtcgagcgcg
    13741 gagatcgcgt tgtcgagccg gatgtcccag tacgcgagct cggcgtcgaa gctgtcgacc
    13801 cacggccggt cctgcagctg cttggggctg tacgcgacgc tgaggttgaa gctcttcgag
    13861 gtctcggggt tcaacgcgag gttgccgccg gtggtcaccg agatctgctg gttgagctgg
    13921 gtgtagccgc cgtcggccgg cacgccgagc gcgatgcagc gctgcttgac ctcgggggga
    13981 accggtttct tggggtcgtt gaagccgctg cacggatcga ccagcacggc gtcgaaccgc
    14041 gccttgctgc cgtacagctc gccgatgctc ggcgcccgga agccctggcc gaagctgccg
    14101 cggatcacca ggtcggtggt cggcttccag cgcgcgccga tcttgccggt gagcttgaca
    14161 ctgttgagga acgagtagtc cgacagccgc gccgcggcgt tgacgtcgat cagctccgca
    14221 ccgggcttgc cgatgaccag cggggcgcgc agctcggcgt aggcctcacc gaccgagtac
    14281 ttgcccgagg tcggctgcga tggcacgtcg gcgccgtccc ctgccgagac gatcccgtcc
    14341 ggctcgtaga acccgctgag ccggcgaacc tcgacgccga ccgccccggc gagccagccc
    14401 gccggcagct tgaccagatt gcccgacagg ttggccacgg cgtcgaccag cgtctgctcc
    14461 gagacgtcgt gctcggtgaa cgtggtgaac gccagcatgt ccttggtgat cgtgcccttg
    14521 ccgtcggcgc cctggccgcc gaagatgttg aacggcacgc agttggggga gccggggttg
    14581 gccatcgtgc cgcagtggta ccggccatcg ctgcccaggt acgccggccc gagcgcctcc
    14641 tggagcttgg ccgagttgaa cgcgttgctg cggcgctggt cggcgcggtt gagcccgtag
    14701 gccaccgtcg cgtcccagtt gaaccgctgg gtgtcgacgt cgaagtggcc gttggcgccg
    14761 cccgagacgt agaacgtgtt gacgtcctgc tcgaagtgcc gcggcccggc ctcgaccggc
    14821 cggcgggtga ccacgtagtc gttggtcgcc ggatcgagcg tgaacccgaa cgggttgtac
    14881 gggttggtcg cgtcgatctt gatcgtgtcg agccggttgc cgttgccgcc ctcggggccg
    14941 atgaacagcg gctcgggcgc ggcctggttg gccgatacgc ggttggtgaa cgcggcctgg
    15001 ccgcggaggt tgacgttgga cagcagcttg taggtcagcg agctgtacgc ccccatgcgc
    15061 cgcgatggcg tctcgacgta gttgtagggc gagaagttga agcggtccgc ggtcgtgaag
    15121 tcgtgataat cggtgggata caccggggtg ccgacgccgt tgttgagcgt gatgttctgg
    15181 tcctgatcgg tgttggggtc gtggaagatc tcacggccct ggggcgtcgc cgacgagcac
    15241 ttgctggtgc aatggccgat cgccgggatc gggaactggg agatcgtgcg atcggccgac
    15301 gacaccgcgc cctggtcgag gaacgacgcg ccgaccacgg tcgacgtctt gccgttggcg
    15361 ctggtgctgc cccacgacac gtcgtacttc tggacgaagc cgtcgccgtg gttgaagccg
    15421 ccggtgtaga tcgagccgac cgcgccgctg aactccttgc gcgtgatgat gttgatcacg
    15481 ccggcgatcg cgtccgagcc gtagatcggc gacgcaccgt cctccagcac ctcgatgcgc
    15541 tcgacgatgc tcagcgggat cgtgttgagg tcggtcgccg ctgccacgcc gcttcccgac
    15601 gagccgttga cccagcgcac gccgtcgacc agcacgagca cgcgcttgga gccgaggtag
    15661 cgcaggtcgg cctcgaccgc gcctgcgccg atcccgccgc cgtcgggcgg gaacccgaag
    15721 ttgccgctgg agttgaactt gccgttgatc gcgccgcccg agaccggcag ctgctgcagg
    15781 atgtcaccga ccgaggtcag gccggtgcga tcgagatcct cgcgcgacat catcagcacc
    15841 ggggcctgct tgcccagcgg gtcgccgatg cgcgagccgg tcaccatgat gacctcgccc
    15901 tcggtcggcg gcacgacctc ggcggggagg ggcgcgtcgg ccggtgcggg cggcgtggcc
    15961 ggtccggcgg cgtccggcgg cgtcgcgggc gcaggcggtg ccgggggagg ggcgtcggtg
    16021 ggggcgggcg c
//
LOCUS       DASVOR010001563         3327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_366709,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001563 DASVOR010000000
VERSION     DASVOR010001563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3327
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366709"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..491)
                     /locus_tag="VF516_29410"
     CDS             complement(<1..491)
                     /locus_tag="VF516_29410"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8111900.1"
                     /translation="MTAPPTPPSTAPSATLLARTDAIAALTAPGQPFELERRDIAGRT
                     VRVFKHAPPSLRAMYEQYLSDLPFLIYEDERHSFAEAWRAAARIGHVLVHDCGVRAGD
                     RVAIAMRNYPEWILAFTAITSIGAIAVAMNAHWQPDEMAYGLADRGARVLLADQERLD
                     RLAR"
     gene            complement(488..1465)
                     /locus_tag="VF516_29415"
     CDS             complement(488..1465)
                     /locus_tag="VF516_29415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111901.1"
                     /translation="MGATMPSPLFEPPVLCGPLHRPRQMLAEQRYDGHKSIHDDAMAE
                     SLGLRAGPIEGPTHFSQFDPLLYGLYGQAWFETGCLSAHYQNMVVEGEEVRAFVQPAP
                     PGRRLARIWAEKRDGTPVLTGTASVGPRVDGEPAEPAEPTEIEQRIAKLRPPGRLVIY
                     RDLRVGQKGAVAERVRMGAGQHMGDSYPFTLADKLKVITEPCRWYTPGAADSPWGRPI
                     LPREMISVLVSSSSQPNLRGRGPAIGLFAGQEIRLIRGPLFVDHPYDLEREVIALSES
                     PRTESAWIRTRVFDAETRALVAEVILNHAVMKASYAAYDREAAELGRVP"
     gene            complement(1547..1894)
                     /locus_tag="VF516_29420"
     CDS             complement(1547..1894)
                     /locus_tag="VF516_29420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111902.1"
                     /translation="MTALFISYVCDYCDGLAPINWLRGYVVFRGDDDFERPIYVFPSQ
                     TDAAIYRSRNGWHEFPIREVRFEHPISWTKASGSLTGVTLAARPFTLHRDHRFEPLPY
                     HCGLKPLTDSLAA"
     gene            complement(2141..>3327)
                     /locus_tag="VF516_29425"
     CDS             complement(2141..>3327)
                     /locus_tag="VF516_29425"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HEX8111903.1"
                     /translation="WTTDAPGVRRKLTPDDLPGPYATPSVDNGATIVRRPSGALPRVP
                     DGFVVDLLTTKLSEPRMIRTAPNGDVFVVESGAGRVKVVRGADRDAGPERVEVFATGL
                     RQPFGIGFYPPDAPRWVYVANTDSVVRFPYSDGDLVARGRPEVVVDDLSGGGRLRGGG
                     HWTRDLVFSRDGAKMYVSVGSRSNVSDDAAEARRARIFEYTPDGKHERVYATGIRNPV
                     GLAIHPVTGELWTSVNERDELGDHLVPDYITRVRDGGFYGWPWFYIGPHPDPRHRGKH
                     PELRDRVIVPDVLLQSHSASLGMMFYTGTQFPPEYRLQAFAAEHGSWNRARRTGYKVI
                     WAVVRDGVPTGEYVDFMTGMVTADGNVWGRPVAVARTHDGALVVSDDAGSCLWRIRYR
                     GK"
BASE COUNT          465 a         1171 c         1248 g          443 t
ORIGIN      
        1 cgggcgagcc ggtcgaggcg ctcctggtcg gcgagcagca cgcgcgcgcc gcggtcggcg
       61 aggccgtacg ccatctcgtc gggctgccag tgcgcgttca tcgcgaccgc gatcgcgccg
      121 atcgacgtga tcgcggtgaa cgcgaggatc cactcggggt agttgcgcat cgcgatcgcg
      181 acccggtcgc cggcgcgcac gccgcagtcg tggaccagca cgtggccgat ccgcgccgcc
      241 gcgcgccagg cctcggcgaa gctgtggcgc tcgtcctcgt agatcaggaa cggcaggtcg
      301 gagaggtact gctcgtacat cgcgcgcagc gacggcgggg cgtgcttgaa cacccgcacc
      361 gtgcggccgg cgatgtcgcg gcgctcgagc tcgaacggct ggccgggcgc ggtcagcgcc
      421 gcgatcgcgt cggtgcgtgc caggagcgtg gccgacggcg cggtcgacgg tggggtcggc
      481 ggcgcggtca aggcaccctc cccagctcgg ccgcctcgcg gtcgtacgcg gcgtacgatg
      541 ccttcatcac ggcgtggttg aggatcacct cggcgaccag cgcgcgggtc tcggcgtcga
      601 acacgcgggt gcggatccac gccgactcgg tgcgcgggct ctcgctcagc gcgatgacct
      661 cgcgctccag gtcgtagggg tggtcgacga acaaggggcc gcggatcagg cggatctcct
      721 ggcccgcgaa cagcccgatc gccggtccgc ggccgcgcag gttgggctgg ctgctgctcg
      781 acaccagcac gctgatcatc tcgcgcggca agatcggccg tccccacggc gagtcggccg
      841 cgccgggcgt gtaccaccgg cacggctcgg tgatcacctt cagcttgtcg gcgagcgtga
      901 acgggtagct gtcgcccatg tgctggcctg cgcccatgcg gacgcgctcg gccaccgcgc
      961 ccttctggcc gacccgcagg tcgcggtaga tgaccagccg accgggcggc cgcagcttcg
     1021 cgatccgctg ctcgatctcg gtcggctcgg ccggctcggc cggctcgccg tcgacgcgcg
     1081 gcccgaccga ggcggtgccg gtgagcaccg gcgtgccgtc gcgcttctcg gcccagatcc
     1141 gcgcgagccg ccggccgggc ggcgccggct ggacgaacgc gcgcacctcc tcgccctcga
     1201 ccaccatgtt ctggtagtgc gcgctgaggc agccggtctc gaaccaggcc tggccgtaga
     1261 ggccgtacag cagcggatcg aactggctga agtgggtcgg gccctcgatc ggcccggcgc
     1321 gcagaccgag gctctcggcc atcgcgtcgt cgtggatcga cttgtggccg tcgtagcgct
     1381 gctcggccag catctggcgc ggacgatgca gcggcccgca cagcacgggc ggttcgaaca
     1441 gcggagaggg catggtggct cccaccagga gtgggcgcat catcctgcaa cgagcttgcg
     1501 ctgcgcaagc cagcgcgagg ccgcgtgcct cgatccggcg cggcgactag gccgcgagcg
     1561 agtcggtcag cggcttgagg ccgcagtggt acggcagcgg ctcgaagcgg tggtcgcggt
     1621 ggagcgtgaa cggccgggcc gcgagcgtca cgccggtcag gctgccgctg gccttggtcc
     1681 acgagatcgg gtgctcgaag cgcacctcgc ggatcgggaa ctcgtgccag ccgttccggc
     1741 tgcgatagat cgcggcgtcg gtctgcgacg ggaacacgta gatcggccgc tcgaagtcgt
     1801 cgtcgccgcg gaacaccacg tagccgcgca gccagttgat cggcgccagc ccgtcgcagt
     1861 agtcgcagac gtaggaaatg aacagagcgg tcatccttcg cttgcacgcg gggcactgca
     1921 tcggcgagag gctagaagcc cgccggcccg tcgacaagca acgagcgcta accgccgcgt
     1981 gagggacagc ggcaccggcg cgcgggcgct cgtgcgcgac cgacgcccgc ggccgggcgc
     2041 ccgatcaccc gcagtgaagg ccgctcgtgg cagcgacgcc gcgggatccg tggcatccgc
     2101 agggaaccgg ccgacgcgca cgcggcggcg agcggtgcag ctacttgccg cgatagcgga
     2161 tccgccacag gcagctgccg gcgtcgtcgc tcaccacgag cgcgccgtcg tgggtccggg
     2221 cgaccgcgac cgggcggccc cacacgttgc cgtcggcggt gaccatgccg gtcatgaagt
     2281 cgacgtactc gccggtcggc acgccgtcgc gcacgaccgc ccagatcacc ttgtagccgg
     2341 tgcggcgcgc gcggttccac gagccgtgct cggccgcaaa cgcctgcagc ctgtactccg
     2401 gagggaactg cgtgccggtg tagaacatca tgccgagcga cgcggagtgc gactggagca
     2461 gcacgtcggg gacgatcacg cgatcgcgga gctcggggtg cttgccgcgg tggcgcggat
     2521 cggggtgcgg cccgatgtag aaccacggcc agccgtagaa cccaccgtcg cgcacgcgcg
     2581 tgatgtagtc gggcacgagg tgatcgccga gctcgtcgcg ctcgttgacc gacgtccaca
     2641 gctcgccggt caccgggtgg atcgccaggc cgaccgggtt gcggatcccg gtggcgtaga
     2701 cccgctcgtg cttgccgtcg ggcgtgtact cgaagatccg cgcgcggcgc gcctcggcgg
     2761 cgtcgtcgga cacgttcgag cgcgagccga ccgagacgta catcttcgcg ccgtcgcgcg
     2821 agaacacgag atcgcgcgtc cagtggccgc cgccgcgcag ccggccgccg ccggacagat
     2881 cgtcgaccac gacctcgggc cggccgcgcg ccacgagatc gccgtcgctg tacggaaacc
     2941 ggacgaccga gtcggtgttc gccacgtaga cccagcgcgg cgcgtccggc gggtagaacc
     3001 cgatgccgaa cggctggcgc aggccggtcg cgaacacctc gacgcgctcg gggccggcgt
     3061 cgcgatcggc gccgcgcacg accttgaccc gcccggcccc cgactcgacg acgaacacgt
     3121 cgccgttggg cgcggtgcgg atcatgcggg gctcgctgag cttggtcgtc agcagatcga
     3181 cgacgaagcc gtcgggcacg cgcggcagcg cgccggacgg gcggcgcacg atggtcgcgc
     3241 cgttgtcgac cgacggcgtg gcgtacgggc ccggcaggtc gtcgggcgtg agcttgcgcc
     3301 ggacgccggg cgcgtcggtg gtccagt
//
LOCUS       DASVOR010001564         6614 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_542132,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001564 DASVOR010000000
VERSION     DASVOR010001564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6614
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542132"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VF516_29430"
     CDS             <1..763
                     /locus_tag="VF516_29430"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEX8111904.1"
                     /translation="AAPRQLETRIAGESLFNDAIGVVVFTTVLGIASGRAVGAGEVAL
                     VFAREALGGAAFGLVAGYLAYHLLRSIDDYTVEVLITLALAIGGYAAAEALHVSAPLA
                     VIMAGLVIGYRGPRVMSDRTQHYIDAFWKLVDEVLNAVLFMLIGVTVLVLPLYRGMAW
                     AALLAIPIALAARWISVAVPLVAFGRFRQSTPHSIALLTWGGLRGGLSVAMALSLPAS
                     GGRDVILGMTYAVVACSILVQGTTFGPLVRRLTRS"
     gene            complement(766..1146)
                     /locus_tag="VF516_29435"
     CDS             complement(766..1146)
                     /locus_tag="VF516_29435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111905.1"
                     /translation="MTMDALRSGLIALVAVLGGCDVGEVPPGGGPGPDAGSDPRAQKF
                     DSVVKPVVARCRTTGCHLTTPPATGGQPPNFYSYDTLAAVYKMAPGSTNIIITHVGDG
                     QPHNAITYLSTAEKKTIADWIDGK"
     gene            complement(1491..4154)
                     /gene="alaS"
                     /locus_tag="VF516_29440"
     CDS             complement(1491..4154)
                     /gene="alaS"
                     /locus_tag="VF516_29440"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEX8111906.1"
                     /translation="MKAADVRTAFLEFWSRKGHSVVASSSLVPSNDPTLLFTNAGMVQ
                     FKDVFTGAESRGYKRATTSQKCVRAGGKHNDLDEVGKTPRHHTFFEMLGNFSFGDYFK
                     ADAIAWAWELLTSVYRIDPARLCVTVFGGDKDDGLGPDDEARAIWKKVTGFGDDRVIG
                     LGKKDNFWAMGDIGPMGPCSEIHYFMDGVPRRWPTTDPASWIGWLEIWNLVFMQFERR
                     TPGGELFPLPAPSIDTGAGLERVTSVLQGVRSNYDTDLFTGILARAAALAGTRYGKAE
                     DSDTSLRVIADHARCTAFLIADGVFPDNTGREYVLRRIFRRAVRHGKLLGLDRPFMHE
                     VCLQVVAEMGAQYPELVQHQTTIANVALAEEKRFRATLDQGVRLLEDEFAAMHKAGVT
                     EVPGRAVFTLYDTFGFPDDLTEIIAAERGFGVDKLGFNEEMEKARNRSRFTGSDQEAI
                     AGELKAVAGELGATRFTGYDGRGTTGEGVVKAILVDGERVQHASAGATVRLMFDQTPF
                     YAEAGGQIGDTGEVTTDAAAVRIDDTKKPTGDVHVLSGQVTRGTIGVGDRVRFTVDDE
                     RRERIRANHSATHLLHHALKQVLGDHVAQKGSLVAPDRLRFDFAHFSPMTDDEKRRVE
                     DLVNAEIRRNLDSVVEVLPIALAKQRGAVAMFGEKYGERVRVVSIGGESIEFCGGTHV
                     RRAGDIGLFKIVSEAGVAQGVRRIEAVTGAGALDYLRRLEDELGRTGDRLKAAPFEVA
                     QRVDKLLAEQKALDREIDKLKQRLASGGGARDLLSDVVTIKGIKVLAASVEVDDAKVL
                     RDTGDQLRDKLGSGVVVLAGTGGPEVKLVAMVTKDLVGKVPAGKLLGEIAPVLGGRAG
                     GRPDMAQGGGKDTSQVPAALSAARKWVEDHA"
     gene            complement(4186..6120)
                     /locus_tag="VF516_29445"
     CDS             complement(4186..6120)
                     /locus_tag="VF516_29445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111907.1"
                     /translation="MTNSRSIGSISKLLAVAVVVAAVLGAGRVAEAGRKRVVVLDFEG
                     PKSEKFHDDLVRLIKKSHTVVPAEKWNGAAGQLGAGTLSDKDVKKVARKLKIDVIVQG
                     KIEKRRDEFIIRLKLRAGKSGELIGDSIDTKAQGPRIDGRAQKDLKDELVGKIDDIAS
                     NHDSTGDDEDDGPPARRAARPAKGDDDDDKPARKPARNDKAAKAAKDDDDDPPKKTAR
                     KPAQKDNDDDADDDRAARRSKFAGRGDDERGSDKVGKARKPEADDDDELPPKKTARAP
                     DRPARPDKADKADRPDKPDKGDDDDDKLPPRKPAGKASDDAKLAARDPAKKPAARPSS
                     EDDDDKLPPRKPAGKAGAGDDGRRAKAKKVASRDDEAEVEDKAKPIDPRAALSPGERF
                     LDASLGMSFTARRFTFAYSAALRARPNGYSGNPAPGALVDVTAYPLALGHTRRDVVKD
                     LGLELVYDRVLKLSSQAQVQQNGMTTTATYSTLESRFALTAVFRHAFGSSPTAPVVLG
                     TLGYQRQQFNILGTIDLPDVKYSMFVPGAGIRFPVMAKLTLAADAKLLLITSTGAIQD
                     PNWYGAAGVIGFDGMVGADYLITPNLFARAAGRFEVISFSFKGTGSQSNARDGNPMTQ
                     DVFGAHDNYFGGFLTVGYLY"
BASE COUNT          820 a         2332 c         2348 g         1114 t
ORIGIN      
        1 cgccgcgccg cgccagctcg agacccggat cgccggcgag tcgctgttca acgacgcgat
       61 cggcgtggtc gtgttcacca ccgtgctcgg gatcgcgagc ggccgcgcgg tcggcgccgg
      121 cgaggtcgcg ctggtgttcg cgcgcgaggc gctcggcggc gcggcgttcg gcctggtcgc
      181 cggctacctc gcgtaccacc tgctgcgctc gatcgacgac tacacggtcg aggtcctgat
      241 cacgctggcg ctggcgatcg gcggctacgc cgccgccgag gcgctgcacg tgtccgcgcc
      301 gctcgccgtg atcatggccg gcctggtgat cggctaccgc gggccgcggg tgatgtcgga
      361 ccggacgcag cactacatcg acgcgttctg gaagctggtc gacgaggtgc tcaacgcggt
      421 gctgttcatg ctgatcggcg tcaccgtcct ggtcctgccg ctgtatcgcg gcatggcgtg
      481 ggccgcgctg ctcgcgatcc cgatcgcgct cgccgcgcgc tggatctcgg tcgccgtgcc
      541 gctggtcgcg ttcggccggt tccgccagtc gacgccgcac tcgatcgcgc tgctcacctg
      601 gggcgggctg cgcggcgggc tgtcggtcgc gatggcgctg tcgctgccgg cgtccggcgg
      661 ccgcgacgtg atcctcggca tgacctacgc cgtggtcgcg tgctcgatcc tggtccaggg
      721 cacgacgttc ggcccgctgg tgcgccggct gacccgcagc tgacgctact tgccgtcgat
      781 ccagtcggcg atcgtcttct tctccgcggt cgagaggtac gtgatggcgt tgtggggctg
      841 gccgtcgccg acgtgggtga tgatgatgtt cgtcgacccc ggggccatct tgtacacggc
      901 agcgagcgtg tcgtacgagt agaagttggg cggctggccc ccggtcgcgg gcggcgtggt
      961 caggtggcaa cccgtggtgc ggcaccgcgc gaccaccggc ttgaccacgc tgtcgaactt
     1021 ctgcgcacgc gggtcgctac ccgcgtcggg accggggccg cctccgggcg ggacctcgcc
     1081 gacgtcgcag ccaccgagga cagcgaccag tgcgatcaga ccagatcgaa gagcgtccat
     1141 cgtcaccgat cagtatatgc aagactcgac accagctcgc gggcgttcgc cggcaagaag
     1201 ttccggtgag ttgctccgca ctggtggcga gcgcacaccc cgacggagag gtcgccagac
     1261 caaggcgccg ttcaccgcgg cgggtgcagg ccggcgggtg taggccggcc ggtgtgggct
     1321 ggccggtgta ggcttggaac gtgagcgaga agccgcccca gacgtacatc ctgcacaccg
     1381 tcgcactgtt cctcgtgctg gtcacggcga tggtgctgtt ccttcgatac aagtggggct
     1441 gacgcgggct tgccgggccg cgctccccgg gtcggcgcct tcaggcgctg tcaggcgtga
     1501 tcctcgaccc acttgcgcgc ggcgctgagg gccgccggca cctgggaggt gtccttgccg
     1561 cccccctgcg ccatgtcggg ccggccgccg gcccgaccac cgagcaccgg cgcgatctcg
     1621 ccgagcagct tgccggcggg gaccttgccg accaggtcct tggtcaccat cgcgaccagc
     1681 ttgacctccg ggccgcccgt gccggcgagc acgaccacgc ccgagccgag cttgtcgcgc
     1741 agctgatcgc cggtgtcgcg cagcaccttg gcgtcgtcga cctcgaccga cgcggcgagc
     1801 accttgatgc ccttgatcgt gacgacgtcg gacaacaggt cgcgcgcgcc gccgcccgac
     1861 gccaggcgct gcttgagctt gtcgatctcg cggtcgagcg ccttctgctc ggcgagcagc
     1921 ttgtcgaccc gctgcgcgac ctcgaacggc gcggccttga gccggtcgcc ggtgcgcccg
     1981 agctcgtcct cgagccggcg caggtagtcg agcgcgccgg cgccggtgac cgcctcgatc
     2041 cggcgcacgc cctgggcgac gccggcctcg ctgacgatct tgaacaggcc gatgtcgccg
     2101 gcgcgccgca cgtgcgtgcc gccgcagaac tcgatcgact cgccgccgat cgacaccacg
     2161 cggacgcgct cgccgtactt ctcgccgaac atcgcgaccg cgccgcgctg cttggctagg
     2221 gcgatcggca ggacctcgac caccgagtcg aggttgcgcc ggatctcggc gttgaccagg
     2281 tcctcgaccc ggcgcttctc gtcatcggtc atcggcgaga agtgcgcgaa gtcgaagcgc
     2341 agccggtcgg gcgcgaccag cgagcccttc tgggcgacgt gatcgccgag cacctgcttg
     2401 agcgcgtggt gcaggaggtg ggtcgccgag tggttggcgc ggatccgctc gcggcgctcg
     2461 tcgtcgaccg tgaaccggac gcggtcgccg acgccgatcg tgccgcgcgt gacctggccg
     2521 gacagcacgt ggacatcgcc ggtcggcttc ttggtgtcgt cgatccgcac cgcggcggcg
     2581 tcggtcgtga cctcgccggt gtcgccgatc tggccgccgg cctcggcgta gaacggggtc
     2641 tggtcgaaca tcagccggac cgtcgcgccg gccgacgcgt gctggacgcg ctcgccgtcg
     2701 accaggatcg ccttgaccac gccctcgccg gtcgtgccgc ggccgtcgta accagtgaac
     2761 cgggtcgcac cgagctcgcc ggccacggcc ttgagctcgc cggcgatcgc ctcctggtcc
     2821 gagccggtga accggctgcg gttgcgcgcc ttctccatct cctcgttgaa cccgagcttg
     2881 tcgaccccga agccgcgctc ggcggcgatg atctcggtca ggtcgtcggg gaacccgaag
     2941 gtgtcgtaca gcgtgaacac cgcgcggccc gggacctcgg tgaccccggc cttgtgcatc
     3001 gccgcgaact cgtcctcgag cagccggacg ccctggtcga gcgtggcgcg gaaccgcttc
     3061 tcctcggcga gcgcgacgtt ggcgatcgtg gtctggtgct ggaccagctc ggggtactgc
     3121 gcgcccatct cggcgacgac ctgcaggcag acctcgtgca tgaacggccg gtcgagcccg
     3181 agcagcttgc cgtggcggac cgcgcgccgg aagatccggc gcagcacgta ctcgcggccg
     3241 gtgttgtcgg ggaacacgcc gtcggcgatc aggaacgcgg tgcaccgcgc gtggtcggcg
     3301 atcacccgca gcgaggtgtc ggagtcctcg gccttgccgt agcgcgtgcc ggcgagcgcg
     3361 gccgcccggg cgaggatccc ggtgaacaga tcggtgtcgt agttcgaccg cacgccctgc
     3421 agcacgctgg tcacgcgctc gaggccggcg ccggtgtcga tcgacggcgc gggcagcggg
     3481 aacagctcgc cgcccggggt gcggcgctcg aactgcatga acaccaggtt ccagatctcc
     3541 agccagccga tccagctcgc cggatcggtg gtcggccagc gcctgggcac gccgtccatg
     3601 aagtagtgga tctccgagca cgggcccatc gggccgatgt cgcccatcgc ccagaagttg
     3661 tccttcttgc ccaggccgat cacgcgatcg tcgccgaacc cggtgacctt cttccagatc
     3721 gcgcgggcct cgtcgtcggg gccgagcccg tcgtccttgt caccgccgaa caccgtgacg
     3781 cacagccgcg ccggatcgat gcggtagacc gaggtcaaga gctcccacgc ccaggcgatc
     3841 gcgtcggcct tgaagtagtc gccgaacgag aagttgccga gcatctcgaa gaacgtgtgg
     3901 tggcgcgggg tcttgccgac ctcgtcgaga tcgttgtgct tgccgccggc gcgcacgcac
     3961 ttctgcgacg tggtggcgcg cttgtagccg cgggactcgg cgccggtgaa cacgtccttg
     4021 aactggacca tgccggcgtt ggtgaacagc agcgtcggat cattcgacgg cacgagcgag
     4081 ctcgacgcca ccacgctgtg gcccttccgg ctccagaact cgaggaacgc tgtgcgaacg
     4141 tcggcggctt tcatggccga cgaaaactag cacgcagccg gtcggttagt agaggtagcc
     4201 gacggtcagg aacccgccga agtagttgtc gtgggcgccg aacacgtcct gggtcatcgg
     4261 gttgccgtcg cgcgcgttgc tctggctccc ggtgcccttg aacgagaagc tgatcacctc
     4321 gaaccggccg gccgcgcggg cgaagaggtt gggcgtgatc aggtagtcgg cgccgaccat
     4381 gccgtcgaag ccgatcacgc cggcggcgcc gtaccagttg gggtcctgga tcgccccggt
     4441 gctggtgatc agcagcagct tggcgtcggc ggcgagggtc agcttggcca tcaccgggaa
     4501 ccggatcccg gcgccgggca cgaacatcga gtacttgacg tcgggcaggt cgatggtgcc
     4561 caggatgttg aactgctggc gctggtagcc gagggtgccg agcacgaccg gcgcggtcgg
     4621 cgagctgccg aacgcgtggc ggaacaccgc ggtcagcgcg aaccgcgact ccagggtcga
     4681 gtaggtcgcc gtggtggtca tcccgttctg ctgcacctgc gcctgcgagc tcagcttgag
     4741 cacgcggtcg tagaccagct ccaggccgag gtccttgacg acgtcgcggc gggtgtggcc
     4801 gagcgcgagc gggtaggccg tgacatcgac cagggcgccc ggcgcggggt tgcccgaata
     4861 gccgttcggc cgggcgcgca gcgccgcgct gtacgcgaac gtgaaccggc gcgccgtgaa
     4921 cgacatgccg agcgacgcgt cgaggaagcg ctcgccgggc gacagcgcgg cccgagggtc
     4981 gatcggcttc gccttgtcct cgacctcggc ctcgtcgtcg cgcgaggcga ccttcttggc
     5041 cttggcgcgc cggccatcgt cgccggcccc ggccttgccg gccggcttcc tgggcggcag
     5101 cttgtcgtcg tcgtcctcgc tggacggccg cgcggccggc ttcttcgccg ggtccctggc
     5161 ggcgagcttg gcgtcatcgc tggccttgcc cgccggcttc ctgggcggca gcttgtcgtc
     5221 gtcgtcatcg cccttgtccg gcttgtccgg cctgtccgcc ttgtccgcct tgtccggccg
     5281 ggccggcctg tccggcgccc tggcggtctt cttcggcggc agctcgtcgt cgtcgtcggc
     5341 ctcgggcttc ctggccttgc cgaccttgtc gctgccgcgc tcgtcgtcgc cccgcccggc
     5401 gaacttgctg cgccgggccg cccggtcgtc gtccgcgtcg tcgtcgttgt ccttctgggc
     5461 cggcttccgg gcggtcttct tcggcggatc gtcgtcgtcg tccttggccg ccttggccgc
     5521 cttgtcgttc cgggccggct tgcgagccgg cttgtcgtcg tcgtcgtcgc ccttggccgg
     5581 cctggccgct cgccgggccg gcgggccgtc gtcctcgtcg tcgccggtgc tgtcgtggtt
     5641 ggacgcgatg tcgtcgatct tgccgaccag ctcgtccttg aggtccttct gggcccggcc
     5701 gtcgatccgc ggcccctgtg ccttggtgtc gatcgaatcg ccgatcagct cgccgctctt
     5761 gccggcgcgc agcttgagcc ggatgatgaa ctcgtcgcgt cgcttctcga tcttgccctg
     5821 gacgatcacg tcgatcttga gcttgcgggc gaccttcttg acgtccttgt ccgacagcgt
     5881 gccggcgccg agctgcccgg ccgcgccgtt ccacttctcg gccggcacca ccgtgtggct
     5941 cttcttgatc agccggacca gatcgtcgtg gaacttctcg ctcttggggc cctcgaagtc
     6001 gagcaccacg acccgcttgc gccccgcctc ggcgacccgg ccggcgccga gcaccgcggc
     6061 cacgacgacc gcgacggcca gcagcttgct tatcgaaccg atcgatctcg aattcgtcat
     6121 ggccgggtgc tccgagggtg gaatcgccgc tgccgggggt ccaggtggac gcgataacgc
     6181 accccgtcgg gcgcgccgga agcgaggtgg acgtcactcg ccccgagctg ccgggcggcc
     6241 gtcaggactc ggtcgaggat ctggctcatg cccgtggtgg aatacggtac cacagggtgg
     6301 ctggatcggt tgtcgcggcg tgatgaggcg acgccgggcc aagtacgtta cgcttgtcac
     6361 gggatgcgct gcccgagctg cggcaccgat aacgcgccgg attcccggtt ctgcggcggc
     6421 tgtggcacgc gattcgccgt ctcgggccag cgcgtggcgc cgacccaaaa gatctccgac
     6481 gacgcgagct ttccacaacg ccagctctcg cccggcgcag gatctgcggt gccgatcaca
     6541 gcgccggggg tcgtcgcacc gcgcgcggtc tcggccccgc cgttgctcgc ccagagcggc
     6601 ccgccggcgc agcg
//
LOCUS       DASVOR010001565         8904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_45545,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001565 DASVOR010000000
VERSION     DASVOR010001565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8904
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45545"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /locus_tag="VF516_29450"
     CDS             <1..731
                     /locus_tag="VF516_29450"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8111908.1"
                     /translation="TPRRARGRPRGMTEQGAAARRQLYEAAIALITARGYEATTLRDI
                     AKHAGVSTGLLYRYVPNKRAVILALYDELSAEYAARAAAMPPGTWGTRFVFALTTSLG
                     VLAPQRDTLAALTPVLIGTADEGLFAPGTAFSRRRVQAVFFEAVRGASDAPGPGDAAA
                     LARLLYVVHLAVILWWLFDKTPRQRATRDLLALLEGALPMAALALRLEPARNFVRTAD
                     ILCREGLFGDDDDPAPAGAHTEEA"
     gene            728..1627
                     /locus_tag="VF516_29455"
     CDS             728..1627
                     /locus_tag="VF516_29455"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8111909.1"
                     /translation="MSARATRSIPALVPPEIGRVRFDVGKTIWLWGMAIPGLVAGIPS
                     ATPATLAMSLVLTFLTLCVGHSVGLHRGIIHRTYQARPAVRGALAYLFVLSGLGGPLS
                     WARLHAVRDLWQNRADCPRYFAYRHSAVRDFVWNLHLRFEPADDRASARLPPDVLGDR
                     WLRFLERTWPLHVLALALGVFAVLGPGGVAVCVCARTAASIAGHWFVGYAAHAWGERR
                     FKIPGAAESGTNVWLLGVLSFGEGFHNNHHALPRSARMGMRAHELDLGWLVIRALEAV
                     GLVRGVSAWHRAGAPPPAAVPAP"
     gene            1716..2621
                     /locus_tag="VF516_29460"
     CDS             1716..2621
                     /locus_tag="VF516_29460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111910.1"
                     /translation="MTRRWITESLGADAFEQLATGLAGSELSSVLLEVMRQRAEARTP
                     AEVLAQYQRDAFCRPAAIDQRVSLEIDRHLLAAADGFEAIELSPVAPLAACSRVARTG
                     QNRVLSALRSTEIVADPTNVLALECAARLRAGATAVHLATSQRVIRTQPVPKRPGMAS
                     HFRIFVLASGGIEAKDHAFTAAALERHIRTMLAALDRLEAHGYAFGARRVDVLATPAR
                     AALADRIAGSLDGIAVHRKPLDHPYYSAGLRYMLWVTAPDGAELPLIDGGAFDWLAQL
                     ASNRCAAYVATGAGAQLIAFRFRAR"
     gene            complement(2660..3820)
                     /locus_tag="VF516_29465"
     CDS             complement(2660..3820)
                     /locus_tag="VF516_29465"
                     /inference="COORDINATES: protein motif:HMM:NF018180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEX8111911.1"
                     /translation="MHIEPFQVAIPQADLDDLRARLERTRWSPELANDDWRYGTRGDY
                     LRELVAYWRSGFDWRAQERAINAVTQFRTSIDGLPIHFVHVRGRGPRPIPLLLGHGWP
                     WTFWDLKKVIGPLSDPAAHGGDPGDAFDVVVPSLPGYAFSTPLTVPGINYWRTADLWV
                     QLMERLGYPRFAAQGGDWGALVAAQLGHKHADRLIGVHLHFCTPLTALAGRPLDASEY
                     GDEIHLLEHNTRFLRDGSGYASIQATRPQTLAHGLDDSPVGLCAWLVEKRRSWSDCGG
                     DVERVFSRDELLTTVCLYWLTHSFGSSARFYYEAAHHPWRPSHDRQPVVEAPTGVARF
                     PRDVLVMPRRWIERYYNLQRYTEMPRGGHFAPMEEPALLVDELRSFFRPLRG"
     gene            complement(3892..4998)
                     /locus_tag="VF516_29470"
     CDS             complement(3892..4998)
                     /locus_tag="VF516_29470"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8111912.1"
                     /translation="MRVCNDGSEVQCHARVLVDADGNLRPNATPAGFSPAQLRSFYGI
                     TSNGSTSTTVAIVDAFAYPRALSDLQTYRSTFGLPAISACNGSVHPCFAVVGQTCGSP
                     PATTNLGWQQETALDLDMVSAMCPNCNILLVEGSSSSFSNLAAAANRAATCTTAGSAP
                     VAISNSYGGSESGGSSLSGSYTHPGITVTASSGDSGFQAGPQFPATSPGVLAIGGTSV
                     HMGSTPRETVWKTTSTEATGSGCSTVFAKPSWQHDAGCSRRMEADVSAVADPNTGVAV
                     FAPTSSTASAFQVFGGTSVAAPLIAGIVGSTGAGIDPSTVYSRVAANRALLFDVTSGN
                     NGTCTGGTTAYFCNAATGFDGPTGLGSPVGPGAF"
     gene            complement(5043..6710)
                     /locus_tag="VF516_29475"
     CDS             complement(5043..6710)
                     /locus_tag="VF516_29475"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX8111913.1"
                     /translation="MARRWGALALIAAGALGACASEPMNPGPIPPALSIPRLQTPAAN
                     AYVGSVHAAGSLRPTFRWAATSEDPAVSYRLQISSEASFTAFVTTIPTATTSYQPAID
                     LPVDRTVPVGTRYYWRVCACRDTTCWPYSPARALNLGRSDRDVNGDGFADVVVGSAGY
                     ASDTVGKVYVYFGGAEPTLDPIPDGTLVAPTTKDFFGTAVAIAGDINGDGFADIIVGA
                     SDEGNGGAGAAYLYYGGPGPVFDGTADQVLHGVVPGDLFGLTVASAGDVNGDGFADVL
                     IGAPWADGMKGTVYVGLGGLGGISTSRVLSGTSTKDPYERSFGLGVSSIGDLDGDGYG
                     DLAIAINTVTGVAGYLYFGDPTTSLDAPIAIRAGGVGPIVGVGDLDGDGFADLAIQDQ
                     GSAGGDAVTLHFGNERRDLAGAGVTITQQEPGFGASIAPAGDINGDGFADVVVGADPD
                     YEGRFGGAFLYLGGPQGMFANVAAGSILGTPSRFASWNVAPAGDVNGDGVDDIVIGGG
                     IVPASPVQRQVWVYQGQAINVFQRTPYGTLTDLTDPPNIDYGVVVAH"
     gene            complement(6871..7356)
                     /locus_tag="VF516_29480"
     CDS             complement(6871..7356)
                     /locus_tag="VF516_29480"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8111914.1"
                     /translation="MRTRTTIFAALAAAVVLLAVNARADKDKAGFIRITPDEVKWMER
                     PGYDGVKFAVIQGDPSKPGLYVIRAKFSPGAMTRPHWHPEDRFVTVMQGTWYTGEGDA
                     FEPDKTVPLKAGSFMMHPAKAHHYDGAKDEEVIVQIVGIGPSATNLVDPKQGNTGRSK
                     K"
     gene            complement(7466..>8904)
                     /locus_tag="VF516_29485"
     CDS             complement(7466..>8904)
                     /locus_tag="VF516_29485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8111915.1"
                     /translation="LHNQRLTLVDARSGAVRQVSPPELYLYEYDWSPDGKAFALTAAP
                     GPGDNNWWTAQLYTMPADTGAVTAIHKPALQIAQPRWSPDGAAIGFIGGLMSDQGVTG
                     GDVFTVPAAGGAAINRTAERRSTPSAFTWLSPSRMLVAEYVGGASALGTLDLATGALD
                     RLWQGDEAFHGGRFGNHAVSRDGKVIAAIRSDWQHPPEIWAGPLGAWKPITSLNAGHR
                     APWGEAKSITWTSDGFTVQGWLVYPHDFDPARRYPMIVSPHGGPASVTTPRWPRQGFD
                     PIAYSALGYFVLLPNVRGSYGQGEAFTRANVKDFGGGDLRDLLAGVDAAVKQAPIDPG
                     RLGLTGWSYGGYMTMWTITQTHRFKAAVAGAGIANWQSYYGQNAIDQWMIPYFGASVY
                     DDPAVYARSAPIAFVKRVTTPTLIIAGERDGECPPPQSYELWHALKTLGVPTALVVYA
                     GEGHMFRDTAHKRDVLERAAAWFDKYLR"
BASE COUNT         1218 a         3250 c         3155 g         1281 t
ORIGIN      
        1 caacgccccg gcgagcacgg ggccgcccgc gcggcatgac cgagcagggc gccgcggccc
       61 ggcgccagct ctacgaggcc gcgatcgccc tgatcacggc gcgtggctac gaggccacga
      121 cgctgcgcga catcgcgaag cacgccggcg tcagcaccgg gctgctgtat cgctacgtcc
      181 cgaacaagcg cgcggtcatc ctcgcgctgt acgacgagct gtcggcggag tacgccgccc
      241 gcgccgcggc gatgccgccg ggcacgtggg gcacgcgctt cgtgttcgcg ctgaccacca
      301 gcctcggcgt cctcgcgccc cagcgcgaca cgctcgcggc gctgacgccc gtcctgatcg
      361 gcaccgccga cgaggggctg ttcgcgccgg gcaccgcgtt ctcgcgccgg cgggtgcagg
      421 cggtgttctt cgaggcggtc cgcggcgcgt ccgatgcccc cggccccggc gacgccgcgg
      481 ccctcgcccg gctgctctac gtcgtccacc tcgcggtgat cctgtggtgg ctgttcgaca
      541 agacgccgcg ccagcgggcg acgcgcgacc tgctcgcgct gctcgagggc gcgctcccga
      601 tggccgcgct cgcgctccgc ctcgagcccg cgcgcaactt tgtccggacc gcggacatct
      661 tgtgtcgcga ggggctgttc ggtgacgacg atgaccccgc tccggcgggg gcacacacgg
      721 aggaagcatg agtgcacgtg cgacacgatc catccctgcg ctggttccac ccgagatcgg
      781 gcgggtccgg ttcgatgtcg gcaagacgat ctggctgtgg ggcatggcga tccccggcct
      841 ggtcgccggc atcccgtccg caacgccggc gacgctcgcg atgtcgctgg tgctgacgtt
      901 cctcaccctg tgcgtcggcc actcggtcgg cctgcaccgc ggcatcatcc accggaccta
      961 ccaggcgcgc ccggccgtgc gcggcgcgct cgcgtacctg ttcgtgctgt ccggcctggg
     1021 tggcccgctg tcctgggcgc gcctccacgc ggtgcgcgat ctgtggcaga accgcgccga
     1081 ctgcccgcgc tacttcgcgt accgccactc ggcggtgcgc gacttcgtgt ggaacctgca
     1141 cctccgcttc gagcccgcgg acgatcgggc cagcgcccgc ctgccgcccg acgtcctcgg
     1201 cgaccggtgg ctgcggttcc tcgagcgcac ctggccgctc cacgtcctgg cgctcgcgct
     1261 cggcgtgttc gccgtgctcg gccccggcgg cgtcgcggtc tgcgtctgcg cgcgcaccgc
     1321 ggcgagcatc gccggccact ggttcgtcgg ctacgccgcg cacgcgtggg gcgagcgccg
     1381 cttcaagatc cccggcgccg ccgagagcgg cacgaacgtc tggctgctcg gcgtgctgtc
     1441 gttcggcgag ggcttccaca acaaccacca cgccctgccc cgctcggcgc ggatgggaat
     1501 gcgcgcgcac gagctcgacc tcggctggct cgtgatccgt gcgctcgagg cggtgggcct
     1561 ggtccgcggc gtctcagcgt ggcaccgcgc cggggcgccc ccgccggctg ccgtccccgc
     1621 gccctgacgt cagccgtccg aaccgcaacg ccgcggacgc gccaggtcct cggcgccgcg
     1681 cagggggcct tcctcccgcg tgttagcgtc cgggcatgac ccggcgctgg atcacagaga
     1741 gcctcggggc cgatgcgttc gagcaactcg ccaccgggct ggccggcagc gagctgtcct
     1801 cggtgctgct cgaggtgatg cggcagcgcg ccgaggcgcg gacgcccgcc gaggtgctcg
     1861 cccagtacca gcgcgacgcg ttctgtcgac cggccgcgat cgaccagcgc gtcagcctcg
     1921 agatcgaccg ccacctgctc gccgccgccg acggcttcga ggcgatcgag ctgtcgccgg
     1981 tcgcgccgct cgcggcgtgc tcccgcgtgg cgcgcaccgg ccagaaccgc gtgctgtcgg
     2041 ccctgcgctc gaccgagatc gtcgccgatc cgaccaacgt gctggcgctc gagtgcgcgg
     2101 cgcggctgcg cgccggcgcc accgccgttc acctcgcgac cagccagcgc gtgatccgca
     2161 cccagccggt accgaagcga ccgggcatgg cgtcgcactt ccggatcttc gtgctcgcca
     2221 gcggcggcat cgaggccaag gaccacgcgt tcaccgccgc cgcgctggag cgccacatcc
     2281 gcaccatgct ggcggcgctg gaccgcctgg aggcccacgg atacgcgttc ggcgcccgcc
     2341 gcgtcgatgt gctggccacg ccggcgcgcg ccgcgctcgc cgaccgcatc gccgggtcgc
     2401 tcgacggcat cgcggtccac cgcaagccgc tggaccaccc gtattactcg gcgggcctgc
     2461 gctacatgct ctgggtcacc gcgcccgacg gtgccgagct cccgctgatc gacggcggcg
     2521 cgttcgactg gctcgcgcag ctggcgtcga accggtgcgc cgcgtacgtc gcgaccggcg
     2581 ccggcgcgca gctgatcgcg ttccggttcc gcgcgcgctg aggcgagcgc cggcgctgac
     2641 gacgcgaccg cggccgggat cagccgcgca gcgggcggaa gaagctgcgg agctcgtcga
     2701 ccaggagcgc cggctcctcc atcggcgcga agtggccgcc gcgcggcatc tcggtgtagc
     2761 gctggaggtt gtagtagcgc tcgatccagc gccgcggcat gacgagcacg tcgcgcggga
     2821 accgcgccac gccggtcggg gcctcgacga cgggctggcg atcgtgcgac ggccgccacg
     2881 ggtggtgagc ggcctcgtag tagaaccgcg ccgagctccc gaagctgtgc gtcagccagt
     2941 acagacagac cgtggtgagc agctcgtccc gggagaacac gcgctcgacg tcgccgccgc
     3001 agtcgctcca gctccgccgc ttctcgacca gccaggcgca cagcccgacc ggcgagtcgt
     3061 cgagcccgtg cgcgagggtc tgcggccggg tggcctggat gctcgcgtag ccgctcccgt
     3121 cgcggaggaa ccgcgtgttg tgctccagca ggtggatctc gtcgccgtac tcgctggcgt
     3181 cgagcgggcg ccccgcgagc gcggtgagcg gcgtgcagaa gtggaggtgg accccgatca
     3241 gacggtcggc gtgcttgtgg ccgagctgcg ccgcgaccag cgcgccccag tcgccgccct
     3301 gcgccgcgaa ccgcgggtag ccgagccgct ccatcagctg cacccagaga tcggcggtgc
     3361 gccagtagtt gatgcccggg acggtcagcg gggtcgagaa cgcatagccc ggcagcgacg
     3421 ggacgacgac atcgaacgcg tcgccgggat cgccgccgtg cgccgcggga tccgacagcg
     3481 ggccgatgac cttcttcaga tcccagaacg tccacggcca gccgtggccg agcagcagcg
     3541 ggatcgggcg aggcccgcgg ccgcgcacgt ggacgaagtg gatcggcagg ccgtcgatcg
     3601 acgtgcggaa ctgggtgacc gcgttgatcg cgcgctcctg cgcgcgccag tcgaagcccg
     3661 atcgccagta cgcgacgagc tcgcgcaggt agtcgccgcg ggtcccgtag cgccagtcgt
     3721 cgttggcgag ctccggcgac caccgcgtgc gctccaggcg ggcgcgcagg tcatcgagat
     3781 cggcctgggg aattgcgacc tggaacggtt cgatgtgcat gggggctgcg cctcgcgtgg
     3841 aagcgtatca gcaccccggg tcgcggtcgc cggcagcggc gccgcgcagc gctagaacgc
     3901 gcccgggccc accggcgacc cgaggccggt cgggccgtcg aatcccgtcg cggcgttgca
     3961 gaagtacgcc gtggtcccgc cggtgcaggt gccgttgttg cccgaggtga cgtcgaacag
     4021 cagggccctg ttcgcggcga cgcgggagta gacggtcgac gggtcgatcc cggcgcccgt
     4081 gctgccgacg atgccggcga tcagcggcgc ggcgacgctg gtgccgccga acacctggaa
     4141 cgccgacgcg gtcgacgacg tcggggcgaa caccgccacg cccgtgttgg gatccgcgac
     4201 cgccgagacg tcggcctcca tgcgccggct gcagcccgcg tcatgctgcc agctcggctt
     4261 ggcgaacacc gtgctgcacc cgctgcccgt ggcctccgtg ctggtcgtct tccagacggt
     4321 ctcgcgcggc gtgcttccca tgtggacgga cgtgccgccg atcgcgagga cgccaggcga
     4381 cgtcgccggg aactggggac ccgcctggaa gccgctgtcc cccgagctcg cggtgaccgt
     4441 gatgccgggg tgggtgtacg accccgacag cgagctaccg cctgactcgc tgccaccgta
     4501 gctgttgctg atcgccacgg gtgcgctgcc ggccgtcgtg cacgtggccg cccggttggc
     4561 cgcggccgcg aggttcgaga acgacgagct ggagccctcg acgagcagaa tattgcagtt
     4621 ggggcacatc gcgctcacca tgtcgagatc gagcgccgtc tcctgctgcc agccgaggtt
     4681 ggtggtcgcc ggcggcgagc cgcaggtctg gccgacgacg gcgaagcaag ggtggaccga
     4741 cccattgcac gccgagatcg ccggcagtcc gaacgtcgag cggtacgtct gcagatccga
     4801 cagcgctctg ggataggcga acgcgtcgac gatcgcgacc gtcgtcgacg tgctgccgtt
     4861 gctggtgatg ccgtagaacg agcgcagctg ggccgggctg aagcccgccg gcgtcgcgtt
     4921 gggccggagg ttgccgtccg cgtcgaccag gacgcgggcg tggcattgca cctcgctgcc
     4981 gtcgttgcag acccgcatgt tgggcgcgcc aggcggacac gaaggtcatc gaagcgagca
     5041 ggtcagtgcg cgacgacgac cccgtagtca atgttcggcg ggtccgtcag gtcggtgagc
     5101 gtgccgtagg gcgtccgctg gaacacgttg atcgcctggc cctggtacac ccacacctgc
     5161 ctttgaaccg ggctggccgg aacaatcccg cccccgatca cgatatcgtc cacaccatcg
     5221 ccgttgacgt ctccggccgg cgccacgttc cacgaggcga accggcttgg tgtgccgagg
     5281 atcgagcccg cggcgacatt cgcgaacatc ccctggggcc cgccgaggta caggaacgcg
     5341 cctccgaacc tcccctcgta gtcggggtcg gcgccgacga cgacgtcggc gaagccgtcg
     5401 ccgttgatat cccccgccgg cgcgatcgat gcaccgaacc cgggctcttg ctgggtgatc
     5461 gtgacgccgg cacccgcgag gtcgcgccgc tcgttcccga agtggagagt caccgcatct
     5521 ccgccggcgc tcccctgatc ttggatcgcg aggtcggcga agccgtcgcc gtcgagatcg
     5581 ccgacgccga cgatcggacc cacgcccccc gcgcggatcg cgatcggcgc atcgagcgac
     5641 gtcgtgggat cgccgaagta gagatatccc gcaacgccgg tcacggtatt gatggcgatc
     5701 gccagatcgc cgtagccgtc gccgtcgagg tcgccgatcg aggacacccc caggccgaag
     5761 gacctctcgt acggatcctt ggtggacgtc ccgctcagca cacggctggt cgagatccca
     5821 ccgagtccac cgagccccac gtacaccgtg cccttcatgc cgtctgccca cggggctccg
     5881 atgaggacgt ccgcgaaccc gtcgccgttg acgtcgccgg cggaagcgac cgtcaatccg
     5941 aacaggtccc cgggaaccac accgtggagc acctgatcag ccgtgccatc gaacaccggc
     6001 ccgggtccac cgtaatacag gtaggctgcg ccggctccgc cattgccctc gtcggaggcg
     6061 ccgacgatga tgtcggcgaa gccatcgccg ttgatgtccc ccgcgatcgc cacggcggtc
     6121 ccgaagaagt ccttggtcgt cggcgccacc agggtaccgt cggggatggg gtcgagggtc
     6181 ggctccgcac cgccgaagta gacgtagacc ttgcccacgg tgtcactggc gtagcccgcc
     6241 gagcccacca cgacgtcggc gaagccgtcc ccgttgacgt cgcgatcgct gcggccgagg
     6301 ttcagcgccc gcgccggcga gtacggccag caggtcgtgt cgcggcacgc gcagacccgc
     6361 cagtagtatc gcgtgccgac ggggacggtc cggtccaccg gcaggtcgat cgccggctga
     6421 tagctcgtcg tggcggtcgg aatggtcgtg acgaacgccg tgaacgaggc ctcggagctg
     6481 atctgcagcc ggtacgacac cgcaggatct tcggacgtgg cggcccaccg gaaggtcggc
     6541 cggagcgagc ccgccgcgtg gaccgatccg acgtacgcgt tggcggccgg cgtctggagc
     6601 cggggaatcg acagcgccgg cgggatcggc cccgggttca tcggctcgga ggcgcatgcc
     6661 cccagcgcgc cggccgcgat cagcgcgagc gcgccccacc ggcgagccat cgccggcccc
     6721 tgccccagga tccctgtcca tccagcaaca actccgacga cgtgcatgac ccctccgcga
     6781 tgcgaggtga acacgagctc gcccggcgca gcaagcccga cgcgcgatcg cggcgccgcg
     6841 atcgcgtgga aaacgctggc aatggcgcga ctacttcttc gaacggccgg tattgccctg
     6901 cttgggatcg accaggttgg tcgcgctcgg gccgatgccg acgatctgca cgatgacctc
     6961 ttcgtccttg gcgccatcgt agtggtgcgc cttcgcgggg tgcatcatga agctgcccgc
     7021 cttgagcggc accgtcttgt cgggctcgaa ggcatcgccc tcgccggtgt accaggtgcc
     7081 ctgcatcact gtcacgaagc gatcctcggg gtgccagtgc ggccgggtca tcgcgcccgg
     7141 cgagaacttg gcgcggatca cgtagagccc gggcttggac gggtcgccct ggatcacggc
     7201 gaacttgacg ccgtcgtagc ccggccgttc catccacttg acctcgtcgg gcgtgatgcg
     7261 gatgaagccg gccttgtcct tgtcggcgcg cgcgttcacc gcgagcagga cgacggccgc
     7321 tgccagggcc gcgaaaatcg ttgtcctcgt acgcatgtgt cctcctggga gatgatgatg
     7381 gaccgggggc gccggcagtc tgcacgatcg gcctgccgcg acgcaacccg gacgccgtca
     7441 cgcttcggca ccggcgccgc cgagctcacc gcaggtactt gtcgaaccac gccgccgcgc
     7501 gctcgaggac gtcgcgcttg tgcgcggtgt cgcggaacat gtggccctcg ccggcgtaca
     7561 ccacgagcgc ggtcggcacg ccgagcgtct tgagcgcgtg ccacagctcg tacgactgcg
     7621 gcggcggaca ctctccgtcg cgctcgccgg cgatgatcag ggtcggcgtg gtcacccgct
     7681 tgacgaacgc gatcggcgcg ctcctggcat agaccgccgg atcgtcgtac accgacgcgc
     7741 cgaagtacgg gatcatccac tgatcgatcg cgttctgacc gtaatagctc tgccagttcg
     7801 cgatcccggc gccggcgacc gccgccttga accggtgggt ctgggtgatc gtccacatcg
     7861 tcatgtagcc gccgtagctc cagccggtga ggccgagccg gcccggatcg atcggcgcct
     7921 gcttcaccgc cgcgtcgaca ccggcgagca ggtcgcgcag gtcgccgccg ccgaagtcct
     7981 tgacgttggc ccgcgtgaac gcctcgccct ggccgtagct gccgcgcacg ttgggcagca
     8041 gcacgaagta gccgagggcc gagtacgcga tcggatcgaa cccctggcgc ggccagcgcg
     8101 gcgtggtgac cgacgccggc ccgccgtgcg gcgacacgat catcggatag cgccgggcgg
     8161 ggtcgaagtc gtgggggtac accagccagc cctgcacggt gaagccgtcg ctggtccagg
     8221 tgatgctctt ggcctcgccc cacggcgcgc ggtggccggc gttgagcgac gtgatcggct
     8281 tccacgcgcc gagcggcccc gcccagatct ccggtgggtg ctgccagtcc gagcggatcg
     8341 cggcgatgac cttgccgtcg cgcgacaccg cgtggttgcc gaacctgccg ccgtgaaatg
     8401 cctcgtcgcc ctgccacagc cggtcgagcg cgccggtcgc gaggtcgagc gtgccgagcg
     8461 cgctcgcccc gccgacgtac tcggcgacca gcatccgcga cggcgatagc caggtgaacg
     8521 cgctcggcgt cgaccgccgc tcggccgtgc ggttgatcgc ggcgccgccg gcggcgggca
     8581 cggtgaacac gtcgccgccg gtgacgccct gatcgctcat cagcccgccg atgaagccga
     8641 tcgccgcgcc gtccggcgac caccgcggct gggcgatctg cagcgctggc ttgtggatcg
     8701 cggtcaccgc gccggtgtcg gccggcatgg tgtagagctg cgcggtccac cagttgttgt
     8761 cgccgggccc cggcgcggcg gtcagcgcga acgccttgcc gtcgggcgac cagtcgtact
     8821 cgtagagata cagctccggc ggcgatacct ggcgcaccgc gcccgaccgc gcgtcgacca
     8881 gggtcagccg ctggttgtgc agat
//
LOCUS       DASVOR010001566         3560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_308515,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001566 DASVOR010000000
VERSION     DASVOR010001566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3560
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308515"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VF516_29490"
     CDS             complement(<1..553)
                     /locus_tag="VF516_29490"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8111916.1"
                     /translation="MTGNWIDGQGDALRRSISLLTCRAGAWRPHRDEWLGHLLAEYYW
                     SLARYANISISERGILVAAKTLPGGTFTMARDLTVTRMGYGAMQLAGPHVFGPPADRA
                     AAVAVLREVVALGITHIDTSDYYGPHVTNQIIKEALYPYPEELHIVTKVGAERDAEGN
                     WPQALAPEQLRQAVYDNLHNLGLD"
     gene            complement(840..2084)
                     /locus_tag="VF516_29495"
     CDS             complement(840..2084)
                     /locus_tag="VF516_29495"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8111917.1"
                     /translation="MTKSHVHTTPPLDIASTNFKADPWPACAHLREEQPVARLPVRVH
                     FGMDEAYLVTRYEDAAMVLRDERFVKDIRSARNTHDLGLPWMPRALKPLAHTILDSDG
                     EEHRRLRTLVRDTFAPKHIAQLEPQVRELVGTLLDRMAVADRVDLIADFALPLPLTVI
                     STILGVPERDRLRFRRWFSSLIGAGASNRPSLSMLLKLPDLFAMMRFLRRLVAERRAH
                     PGDDLVSRLALAQEGGDRLSEDELVAMVGILLIAGYETTVNLIATGTLLLLGHPDQLA
                     RLREDPALIDSAVEELLRLATPVDVATERYAREEVEIAGVTIPRGALVLVAIVSANAD
                     ETRFPQAERMDVSRAENRHLAFGLGPHYCLGAPLARMEARVALKALIQRFPNLRLAAP
                     AEQLQWRPGVSLRGLVSLPVSL"
     gene            complement(2357..3034)
                     /locus_tag="VF516_29500"
     CDS             complement(2357..3034)
                     /locus_tag="VF516_29500"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8111918.1"
                     /translation="MPDEPEAKNSAELIQAIQAQLAAARRTQILDAATRVFASKGFNR
                     ATIRDVAQAAGVADGTIYNYFANKTDLLFGLLDRLNDTERRPASLVQATEAPFADYFR
                     AYVRERVEALWSNADLFRAVLPELLANPALRARYCDEVIAPTMVLGEAAFRALVANGT
                     VRDLDLALSVRIVAGSVLGLMVLQLLGDETLAARWRELPDAIADLLLPGLTPMPGPPA
                     PTRNDAS"
     gene            complement(3328..>3560)
                     /locus_tag="VF516_29505"
     CDS             complement(3328..>3560)
                     /locus_tag="VF516_29505"
                     /inference="COORDINATES: protein motif:HMM:NF014592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HEX8111919.1"
                     /translation="ADAVVVPYRGRTSLFIQPGDHFRAVDVMLTNFHLPRSTLIVLVS
                     AFASRDLILRAYAEAVARGYRFYSFGDAMLIV"
BASE COUNT          615 a         1116 c         1216 g          613 t
ORIGIN      
        1 cgtcaaggcc gagattgtgg aggttgtcgt acacggcctg acgcagctgc tcgggagcga
       61 gcgcctgggg ccagttgccc tcggcgtcgc gctccgctcc gaccttggtg acgatgtgca
      121 gctcttcggg gtaggggtac aaggcttcct tgatgatctg gttggtgaca tgcggcccgt
      181 agtagtcgct ggtgtcgatg tgtgtgatcc ccagcgccac cacctcgcgc aacacggcca
      241 cggcggcggc acggtcggcc ggtgggccga agacgtgcgg accagccagt tgcatcgcgc
      301 cgtaccccat gcgtgtgact gtcagatccc tggccatggt gaacgttccg ccgggaagtg
      361 tcttcgctgc cacaagaatt cctctctcac tgatactgat gtttgcgtag cgggctaaag
      421 accaataata ctcagccagc agatgcccaa gccactcgtc tcgatgtggc cgccacgcac
      481 cagcgcggca ggtcagcagg ctaatagagc gccgcagtgc gtctccctgc ccgtctatcc
      541 agttgcccgt cacggtctac cctgatcata gagcagaagg tgtgccggag catatataac
      601 ggcgcgcatg aggcagccgt cagcacggcg gaaagccagg atgcgctgcg cgagctggcg
      661 cggcttgtct tgctgtgccc cggtgcggac gagcctggtc ttagggtgaa cggcaggctt
      721 gctagcgagg tagaggtgtc cgtgttggct cccctcgtgt cgtacaggag caggcgcacg
      781 aatgatggtg cgcctgcgcg ccttacaggc ggcaccgatt cccgcccttc tcacgccacc
      841 tacagtgata ccggcagcga gacgaggccg cgcagcgaga cgccgggccg ccactgcaac
      901 tgctcggccg gcgctgccag gcgcagattc gggaagcgct ggatcagcgc cttgagcgca
      961 acgcgcgctt ccattcgggc gagtggcgcg ccgaggcagt aatgcggacc gaggccgaag
     1021 gccaggtggc ggttctcggc gcggctcacg tccatccgct ccgcctgcgg gaagcgcgtc
     1081 tcgtcggcgt tggccgagac gatggcgacc aggaccagcg cgccgcgcgg gatggtgacg
     1141 ccagcgatct ccacctcctc gcgcgcgtag cgttcggtgg cgacgtccac cggcgtggcc
     1201 agccgcagca gctcctccac cgctgagtcg atcagggccg ggtcctcgcg caggcgagcg
     1261 agttggtcgg ggtggccgag cagcagcaac gtgccggtcg cgatcaggtt gacggttgtc
     1321 tcatacccgg cgatcagtag tatcccgacc atcgccacca gctcatcctc gctcaggcgg
     1381 tcgccccctt cttgcgccaa cgccagccgg ctgacgagat catcaccggg atgggctcgg
     1441 cgctcggcga cgaggcggcg caggaaacgc atcatggcga agaggtcggg caacttcaag
     1501 agcatgctca ggctcggccg attcgacgcg ccggcgccga tgagcgacga gaaccagcgg
     1561 cggaagcgca ggcggtcacg ctcgggcacg ccgagaatcg tggagattac cgtgagcggc
     1621 aggggcagtg caaagtcggc gatcagatcg acccgatcgg ccacggccat tcggtccagc
     1681 agcgtgccga ccagctctcg cacctgtggc tcgagttggg cgatgtgctt gggcgcgaag
     1741 gtgtcgcgaa cgagggtacg cagccgccgg tgttcctcgc cgtcgctgtc gagtatggtg
     1801 tgggcgagtg gcttgagcgc ccgcggcatc cagggcaagc cgaggtcatg cgtgttcctc
     1861 gcgctgcgga tgtccttcac gaagcgttcg tcgcgcagta ccatggccgc gtcttcataa
     1921 cgtgtgacca ggtacgcctc atccataccg aagtgcacgc ggacgggcaa tcgcgccacc
     1981 ggctgctctt cgcgcaggtg ggcgcacgcc ggccagggat cggccttgaa gttggttgat
     2041 gcgatgtcga gaggcggtgt ggtgtggacg tgggacttcg tcatgagcgt ttcctttcgg
     2101 cgcaccagtg gcccaacgcg agctggacga ccttcaccat catgagcgtc accgcgacga
     2161 cgaggtagtc gcgcagcgtc gatcaggtca ttcggatggg tcgtgcccac gcagtcgatc
     2221 acagggcgct atgtcgcgtg agcctttgca caacaccctc tgtcaaacca tcgctcgttt
     2281 ctgtcgcttc cgtcgcttcc gtcgcttccg tcgcttccgt cgctgcgcgg cgccggagcc
     2341 aggcccggcg cgcaggtcag ctcgcgtcgt tgcgcgtggg agctggtggc ccgggcatgg
     2401 gcgtgaggcc agggaggagc agatcagcga tcgcgtcggg aagctcgcgc cagcgcgccg
     2461 cgagcgtctc gtcgccgagc agctgcagca ccatgaggcc gagcaccgat cccgccacga
     2521 tgcgcacgct caatgcaagg tccaggtcac gcaccgttcc gttcgcgact aacgcgcgaa
     2581 aggctgcctc acccaacacc atcgtcggcg cgatcacctc gtcgcagtag cgcgcgcgca
     2641 gcgcagggtt tgcgagcaac tccgggagca ccgcgcgaaa caggtcggcg ttggaccaca
     2701 gcgcctcgac gcgctcgcgc acataagcgc ggaaatagtc cgcgaatgga gcctcagtcg
     2761 cctgaacaag actggccggc cggcgctcgg tgtcgttaag gcggtcgagc aggccaaaga
     2821 gcaggtcggt cttgttggcg aagtagttgt agatcgtgcc gtcagcaacg cctgcagcct
     2881 gcgcgacatc gcgaatggtc gcgcggttga accctttgct ggcgaagacc cgcgtcgccg
     2941 cgtcgaggat ctgcgtccgc cgagcagcgg cgagctgcgc ctggatcgcc tgaatcagct
     3001 ccgcggagtt cttcgcctct ggctcgtctg gcatgtctca accttccctt gcttgaatag
     3061 atattcatcg cggtgaatat ctattcactc gtaatgatag cgcgagattg tcaggctgtc
     3121 aagcgtctgc gcggatatgc ggcaccgctg ccgtgcttac gatgcgcact gggaaggtcg
     3181 agcgccccga acgcgcatca ggcgagcagc aagcgaaatg gacggcgccg gctgagcagg
     3241 gctgaggcct ttatctccct catcggcatc tggctccgcc tcctcggttg acacgaagcg
     3301 agaaatggag ccgtactaaa tactttccta gacgatgagc atggcgtcgc cgaagctgta
     3361 gaagcggtag ccacgcgcga cggcttcggc gtaggcgcgc aggatcaggt cgcgcgaggc
     3421 gaaggcgctg accagcacga tcagggtgga gcgcggcagg tggaagtttg tcagcatcac
     3481 gtcgacggcg cggaagtggt cgccgggctg gatgaagagg gaggtgcggc cgcgataggg
     3541 cacgacgacg gcatcggcgg
//
LOCUS       DASVOR010001567         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_132819,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001567 DASVOR010000000
VERSION     DASVOR010001567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132819"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VF516_29510"
     CDS             <1..241
                     /locus_tag="VF516_29510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111920.1"
                     /translation="PAGKIQITSTIIREALVDVLRGWKAASRAAIDPDQLYSAAKPRL
                     DYQGKRPVACGGSPSAPASAAPGAPDADAGKEPGP"
     gene            253..1635
                     /locus_tag="VF516_29515"
     CDS             253..1635
                     /locus_tag="VF516_29515"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HEX8111921.1"
                     /translation="MFPFVPPSGHAEVDAPDWSPDSWTRRPAQQQAEYPDRAALAAVV
                     EQIGRLPPLVTSWEIEQLRGELARAARGEAFVLQGGDCAESFDECRSEPIAAKLKILL
                     QMSLVLVHGTRKPVVRIGRIAGQYAKPRSADVETRGDVTLPAYRGDLVNRDAFTAADR
                     IPDPALMLRAYERAGLTLNFIRALVDGGFADLHHPEYWDISFAHGSPHAAQYERIVES
                     IRESLDFVSAIAGVESEVLRRVDIFTSHEALALPYEQAQTRRVPRRPGWYNLSTHFPW
                     IGMRTAQLDGAHVEFHRGILNPMGIKIGPAMTTEWLIGLLDALDPHRRPGRITLIHRM
                     GAGQVASKLPPLVEAVRRAGREVLWICDPMHGNTETTSAGIKTRRFDKIVDELEQSFA
                     LHTGLGSRLGGVHIELTGEDVTECTGGARGLSEVDLERAYKSRVDPRLNYEQALECAM
                     RIARHLRGSK"
BASE COUNT          268 a          690 c          665 g          235 t
ORIGIN      
        1 ccccgcgggc aagatccaga tcaccagcac gatcatccgc gaggcgctgg tcgacgtgct
       61 gcgcggctgg aaggcggcgt cgcgcgcggc gatcgacccg gaccagctgt actcggcggc
      121 gaagccgcgg ctcgactacc agggcaagcg cccggtcgcc tgcggcggat caccgagcgc
      181 gccggcgagc gcggcgccgg gtgcgccgga cgccgacgcc ggcaaggagc cgggaccatg
      241 acgatggcat cggtgttccc ctttgtcccg ccatccggcc acgccgaggt ggacgccccg
      301 gactggtcgc ccgacagctg gacgcgccgg cccgcccagc agcaggccga gtaccccgat
      361 cgcgcggcgc tcgccgccgt ggtcgagcag atcggccggc tgccgccgct ggtcacgtcg
      421 tgggagatcg agcagctgcg cggcgagctc gcgcgggccg cgcgcggcga ggcgttcgtg
      481 ctgcagggcg gcgactgcgc ggagagcttc gacgagtgcc gctccgagcc gatcgccgcc
      541 aagctcaaga tcctgctcca gatgtcgctc gtgctggtcc acggcacgcg caagccggtg
      601 gtccggatcg gccggatcgc gggccagtac gccaagccgc gctcggccga cgtcgagacc
      661 cgcggcgacg tcacgctgcc cgcgtaccgc ggcgacctgg tcaaccgcga cgcgttcacc
      721 gccgccgacc gcatccccga tccggcgctg atgctgcgcg cctacgagcg cgccgggctg
      781 accctcaact tcatccgcgc gctggtcgac ggcgggttcg ccgacctcca ccaccccgag
      841 tactgggaca tctcgttcgc ccacggctcg ccgcacgcgg cccagtacga gcggatcgtc
      901 gagtcgatcc gcgagtcgct cgacttcgtg tcggcgatcg ccggggtcga gagcgaggtg
      961 ctgcgccggg tcgacatctt caccagccac gaggcgctcg cgctgcccta cgagcaggcg
     1021 cagacccggc gggtcccgcg ccggcccggc tggtacaacc tgtcgaccca cttcccgtgg
     1081 atcggcatgc ggaccgcgca gctcgacggc gcccacgtcg agttccaccg cgggatcctg
     1141 aacccgatgg ggatcaagat cggccccgcg atgacgaccg agtggctgat cggcctgctc
     1201 gacgccctcg atccccaccg ccggcccggc cggatcacgc tgatccaccg catgggcgcc
     1261 ggccaggtcg ccagcaagct gccgccgctg gtcgaggcgg tccggcgcgc gggccgcgag
     1321 gtgctgtgga tctgcgatcc gatgcacggc aacaccgaga ccacctcggc cgggatcaag
     1381 acccggcggt tcgacaagat cgtcgacgag ctcgagcaga gcttcgcgct gcacaccggg
     1441 ctcggctcgc ggctgggcgg cgtgcacatc gagctgaccg gcgaggacgt caccgagtgc
     1501 accggcggcg cccgcggcct gtccgaggtc gacctcgagc gcgcgtacaa gagccgggtc
     1561 gacccgcggc tcaactacga gcaggcgctg gagtgcgcga tgcggatcgc ccgccacctg
     1621 cgcggcagca agtgatggac cgcggccacc gcggtcgccg cagccgatcg aggaccaagg
     1681 acagcctacc ttcatggcgg acctgaccga cctgatcgtg cggggctggg gcgccctgtc
     1741 gcgctgggcg gtgccggtga tgatgacgct cgcctacgcg ctgctggtcg cgacgtcgga
     1801 gaccgacacc accggcaagg cgtggatggc cgcgggcctc gtgctcgtgc tgaccgcg
//
LOCUS       DASVOR010001568         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_74372,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001568 DASVOR010000000
VERSION     DASVOR010001568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74372"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1445)
                     /locus_tag="VF516_29520"
     CDS             complement(<1..1445)
                     /locus_tag="VF516_29520"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEX8111922.1"
                     /translation="MSAAPTYDAVVIGGGITGAGIARDAALRGLKVALFEKGDYASGT
                     SSKSSKLIHGGLRYLEHGEIGLVFESVSERRVQTRVAPHLVRPLPFLIPIYKGARPGL
                     EMMNVGLWIYDSLALFRAPRLHKVFRGARAALEIEPALRSEGLRGALEYYDCNTDDAR
                     LVLENALDARALGADCHTYTEVIRLERGGDRRITGVAVRDRLTGETRSVTTRVVVLAA
                     GAWTDEMIARFEIPIDRPLLRRTKGVHIVVPRERLPLSRAITLVSPLDHRVMFAIPWR
                     ERTVLGTTDTDFTGTADEVAADADDVRYLCDSANGVFPSAALQPDDVIATWAGLRPLI
                     AAPPNVDESDVSREHEVFTRSDGLVIIAGGKLTTYRRMARQTVHETLKLLDQLGEPVT
                     AARATTKHRPLPGADGLAQADLEGVAAIGRQLMADYRLDVDTATHLCGVYGARAPLLA
                     ARIAADRALGERLDPELPYVWAEIEFAAVHDL"
     gene            complement(1509..>1794)
                     /locus_tag="VF516_29525"
     CDS             complement(1509..>1794)
                     /locus_tag="VF516_29525"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8111923.1"
                     /translation="ASGEAGPSVDAAARPATSPPAASIATSGATAGDVLDLPFKEAKA
                     ALVEGFERDYLTALLARHRGNISRAASEAGIDRNYVHRLVKKYGLEVDRG"
BASE COUNT          227 a          621 c          704 g          242 t
ORIGIN      
        1 agatcgtgga cggcggcgaa ctcgatctcg gcccacacgt agggcagctc gggatcgagg
       61 cgctcgccga gcgcgcgatc ggcggcgatc cgggcggcga gcaggggggc gcgcgcgccg
      121 tagacgccgc acaggtgggt cgcggtgtcg acgtcgagcc ggtagtcggc catgagctgg
      181 cggccgatcg ccgccacgcc ctcgaggtcg gcctgggcca ggccgtcggc gccgggcagc
      241 ggccggtgct tggtggtcgc gcgggccgcg gtgaccggct cgccgagctg gtcgagcagc
      301 ttgagggtct cgtggacggt ctggcgcgcc atccggcggt aggtcgtgag cttgccgccg
      361 gcgatgatca ccaggccgtc gctgcgggtg aacacctcgt gctcgcgcga cacgtcgctc
      421 tcgtcgacgt tgggcggcgc ggcgatcagc ggtcgcaggc cggcccaggt cgcgatcacg
      481 tcgtcgggct ggagcgcggc gctcgggaac acgccgttgg cgctgtcgca caggtagcgg
      541 acgtcgtcgg cgtcggccgc gacctcgtcg gcggtgccgg tgaagtcggt gtcggtcgtg
      601 ccgagcacgg tgcgctcgcg ccacgggatc gcgaacatca cgcggtggtc gagcggcgac
      661 accagcgtga tcgcgcgcga cagcggcagc cgctcgcgcg gcaccacgat gtgcacgccc
      721 ttggtgcggc gcagcagcgg ccggtcgatc gggatctcga accgcgcgat catctcgtcg
      781 gtccacgccc cggcggcgag cacgaccacg cgggtggtga cgctgcgggt ctcgccggtc
      841 agccggtcgc ggacggcgac gccggtgatc cggcgatcgc cgccgcgctc gagccggatg
      901 acctcggtgt aggtgtggca gtcggcgccg agcgcgcggg cgtcgagcgc gttctcgagc
      961 accaggcggg cgtcgtcggt gttgcagtcg tagtactcga gcgcgccgcg caggccctcg
     1021 gaccgcagcg cgggctcgat ctcgagcgcg gcgcgcgcgc cgcggaacac cttgtgcagc
     1081 cgcggggcgc ggaacagcgc gagcgagtcg tagatccaca gcccgacgtt catcatctcc
     1141 aggccgggcc gggcgccctt gtagatcggg atcaggaacg gcagcgggcg gaccaggtgc
     1201 ggcgcgaccc gggtctggac ccgccgctcg gacaccgact cgaacaccag gccgatctcg
     1261 ccgtgctcga ggtagcgcag cccgccgtgg atcagcttgc tcgacttcga cgacgtgccc
     1321 gaggcgtagt cgcccttctc gaacagcgcg accttgaggc cgcgcagcgc cgcgtcgcgc
     1381 gcgatgccgg cgccggtgat gccgccgccg atcacgacgg cgtcgtaggt cggggccgcg
     1441 ctcaccgatc gaacgatacc ggtgcgccgc tctgcgtcaa ggcttggcgg ccgcggcaac
     1501 cggccggctc acccgcgatc gacctcgagg ccgtacttct tcaccaggcg atggacgtag
     1561 ttgcgatcga tgccggcctc ggacgccgcg cgcgagatgt tgccgcggtg gcgggcgagc
     1621 agggcggtga ggtagtcgcg ctcgaagccc tcgaccagcg cggccttggc ctccttgaac
     1681 ggcaggtcga gcacgtcgcc ggcggtcgct ccggaggtcg cgatcgacgc cgccggcggg
     1741 ctggtcgcgg gccgcgcggc ggcgtcgacg gacggcccgg cctcgccgga cgcg
//
LOCUS       DASVOR010001569         3344 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_191708,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001569 DASVOR010000000
VERSION     DASVOR010001569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191708"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..339)
                     /locus_tag="VF516_29530"
     CDS             complement(<1..339)
                     /locus_tag="VF516_29530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111924.1"
                     /translation="MLRPEGAPPSYVCSSCRLVLYDGVDYTWCPACSAVVDWMEMDRP
                     WSPPPPRSGSSSVPGMAEVGLSAMTLIYLGQLVTTALDPLGVALVAPLRLVAVSAAGD
                     FLVAVVASRGE"
     gene            complement(589..1077)
                     /locus_tag="VF516_29535"
     CDS             complement(589..1077)
                     /locus_tag="VF516_29535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111925.1"
                     /translation="MSWSRSFLACLVLTAVMSCGAGGGTSTDAAPGATLSGVGLPCMT
                     AAPGCPSEAPLCMAPFYVSRDPNNGHGYCSKTCLRNTVSITNMNSKFVQLDTPPPSTG
                     DSACPGGTSCTEVYSILPYPDTRPNTTYYSQWACGIQCSADRPCPTGLTCNPDFHRCA
                     PM"
     gene            1198..2436
                     /locus_tag="VF516_29540"
     CDS             1198..2436
                     /locus_tag="VF516_29540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111926.1"
                     /translation="MAVLYRVPAWIFWIAACGMTEPAGHDLVITLYDHPDHVAGDTSN
                     ADMLLVHDGDGLWAGVTGQAGVYHIHLTSDRYGVAVGCKTGTLATTSFVDIRERTVAD
                     GLVLDEPSCYPNSFEAEIDIDVRGIGPGQSAVISVAGNAQVVAKDTTLHLGAVRGPSE
                     LLGTLTDAGGRIVKVIRGPSFEAAGVQSQSLDFTTAGFTPVDHGLSLALSPGDRAKLA
                     TGLIASSDMYPLQMGAAIGASGAYQIAPGLLHDGELFAVSVQVGDRIASFEGKAPDVG
                     QLELPPGLAVSAPMVEIVPYPRPVFSFPPAPGDLPVEEYSFTASTDDAVANHHASWRV
                     ELSTSWLAATRAQDYAIPDLAYAQNFSTRFPLAGHQPIHWELTWIESSAPEPSEGRIT
                     RESTVSGDLPATLSPAASGL"
     gene            2594..>3344
                     /locus_tag="VF516_29545"
     CDS             2594..>3344
                     /locus_tag="VF516_29545"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8111927.1"
                     /translation="MTQRIAPVLAAALSIGCVGPAYHVPRAPTPVATTYKEARGMWKL
                     ATPLDAIPRGRWWEMFHEPELDALEKRLNIDNQTIAQAAESYLQARAQIRAARASYFP
                     TVTADPSVTWFRSAGVTGAGGAAGAAIGSQVSSAIAGRHVVYAAPVEASWAPDLFGRV
                     RYAVHQRQYAAQVSAADLESTRLAAQATLARSYFQLRAQDTLEDELAATVKADQQIAE
                     LTRLQFTAGLATQIAVVQAELTLRTARVQATN"
BASE COUNT          493 a         1161 c         1215 g          475 t
ORIGIN      
        1 ctctccgcgc gacgcgacga ccgcgaccag gaagtcgccg gcggcgctca cggcgaccag
       61 ccgcagcggt gcgacgagcg cgacgccgag cgggtcgagc gccgtggtca cgagctgacc
      121 gagatagatc agggtcatcg ctgacagccc gacctcggcc atgccgggca ccgagcttga
      181 tccggagcgc ggcggcggcg gcgaccatgg ccggtccatc tccatccagt cgaccaccgc
      241 cgagcaagca gggcaccagg tgtagtcgac gccgtcgtag aggacgagcc ggcagctcga
      301 gcacacgtac gacggcggtg cgccttcggg tcggagcacc gggagacgcg gcgacgccgc
      361 cggcctggtc cgcgctggct tcttgcggat cgccggcctc gatggaagcg gagacagccc
      421 tgcgggcccc ctgctcatga ctcttgccaa gcatagccga cgccgggccg ctggcgagcg
      481 atggcttcag ccaccgcgga cctcgggctt ccgggcgctt gcgacgccgt gtctcggccg
      541 cgccgcttcg tgccgcctcg gtcggttccg actcgtcacc cgcgggcgct acatcggcgc
      601 gcagcggtgg aaatcggggt tgcaggtcaa gccggtcgga catgggcggt ccgcgctgca
      661 ctggatcccg cacgcccatt gcgaatagta cgtggtgttc ggccgggtgt cggggtaggg
      721 gaggatgctg tagacctccg tgcaggacgt cccgccggga catgcactgt ctccggtcga
      781 cggaggcggg gtatcgagct gcacgaactt cgaattcatg ttggtgatcg ataccgtgtt
      841 gcgcaggcag gtcttgctgc aatagccatg tccgttgttc gggtcgcgcg agacgtagaa
      901 cggcgccatg cacagcggcg cctcgctcgg gcacccgggc gccgccgtca tgcagggcag
      961 gccgacgccg gacagcgtcg cgcccggcgc ggcatcggtg gacgtgccgc cgccagcgcc
     1021 acaggacatc acagcggtca gcaccaggca cgcgaggaac gagcgactcc aggacatctc
     1081 ggcaccattg tactgcggcg tcatggggtc gatcgtcaaa gccggcgcgc cgcgcgtgcg
     1141 tgagctttgc gtgaacccgt tgccgtgacc ccgccgggcg acgacgatga aggaccgatg
     1201 gccgtgctgt accgggtccc ggcatggatc ttctggatcg ccgcatgtgg aatgaccgag
     1261 ccagcgggcc atgatctggt gatcaccctg tacgatcatc cggaccacgt cgccggcgac
     1321 acgtcgaacg ccgacatgct cctggtccac gacggcgacg gcctgtgggc cggggtcacc
     1381 ggacaggccg gcgtgtacca catccacctc acgagtgacc gctacggggt ggcggtgggt
     1441 tgcaagaccg ggacactggc cacgacgtcg ttcgtcgaca tccgggaacg caccgttgcg
     1501 gacggcctcg tgctcgacga gccgtcgtgt tacccgaact cgttcgaggc cgagatcgat
     1561 atcgacgtgc gcggcatcgg cccgggtcaa tccgccgtga tctcggtcgc cggcaatgcg
     1621 caggtggtcg cgaaggacac cacgctgcac ctcggcgcgg tgcgggggcc gagcgagctg
     1681 ctcggcacgc tcaccgacgc cggcgggcgg atcgtgaagg tgatccgcgg gccgagcttc
     1741 gaggccgcgg gagtgcaatc gcagtcgctc gacttcacga ccgcggggtt cacgccggtg
     1801 gaccacggcc tctcgctggc gctgtcaccc ggcgaccggg cgaagctcgc aaccgggctg
     1861 atcgcgtcga gcgacatgta tcccctgcag atgggcgccg cgatcggcgc gtccggcgcg
     1921 taccagatcg ccccggggct gctgcacgac ggcgagctgt tcgcggtctc ggtgcaggtc
     1981 ggcgaccgga tcgcgtcgtt cgagggcaag gcgccggacg tggggcagct cgagctgccg
     2041 cccgggctgg ccgtgtccgc gccgatggtc gagatcgtgc cgtaccccag gccggtgttc
     2101 tcgttccccc cggcgccggg cgatctcccg gtcgaggagt acagcttcac cgcatccacc
     2161 gacgacgcgg tggccaatca ccacgcgtcc tggcgggtcg agctgtcgac ctcgtggctg
     2221 gctgccaccc gagcgcagga ctacgcgatt ccggatctcg cgtatgcaca gaacttctcg
     2281 acgcgctttc cgctggccgg acaccagccg atccactggg agctgacctg gatcgagagc
     2341 agcgccccgg agccgagcga gggccgcatc acccgggagt ccacggtctc cggggacctg
     2401 cctgcgacgc tctcgcccgc agcgtcgggg ctctgaatcc ggcgcgccga gcgcctccgc
     2461 gtcatccagc gcgccacatc gggcgccgat gacgtgggcg ggtgcacccg gggccgcggc
     2521 gcaccgccag ggcgcgatcc tcgtcgggca tcctgcttgc tggaggtcgg tcggcgagat
     2581 gacggcgccc acgttgacgc agaggatcgc gccggtgctc gcggccgcgc tgtcgatcgg
     2641 ctgtgtcggg ccggcctatc acgtcccgcg cgcgccgacc ccggtcgcga cgacctacaa
     2701 ggaggcccgc gggatgtgga agctcgccac gccgctggac gcgatcccgc gcggccggtg
     2761 gtgggagatg ttccacgagc ccgagctcga tgcgctggag aagcgcctga acatcgacaa
     2821 ccagacgatc gcgcaggccg ccgagagcta cctgcaagcg cgcgcgcaga tccgcgcggc
     2881 gcgcgcgagc tacttcccga cggtgacggc cgatccgtcg gtgacctggt tccggtcggc
     2941 gggtgtgacc ggcgccggcg gggcggcggg cgccgcgatc ggctcgcagg tgagctccgc
     3001 catcgcgggg cgccacgtgg tctacgccgc gccggtcgag gcatcgtggg cgcccgatct
     3061 gttcggtcgc gtgcgctacg cggtgcacca gcggcagtac gcggcgcagg tgagcgcggc
     3121 ggatctggag agcacccggc tggccgcgca ggcgacgctg gcccggtcgt acttccagct
     3181 ccgcgcgcag gacacgctgg aggacgagct cgccgcgacc gtgaaggccg accagcagat
     3241 cgccgagctg acgcggctgc agttcaccgc cggcctcgcg acccagatcg cggtcgtcca
     3301 ggccgagctg acgctgcgga ccgcgcgcgt gcaggccacc aacg
//
LOCUS       DASVOR010001570         5329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395328,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001570 DASVOR010000000
VERSION     DASVOR010001570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5329
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395328"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VF516_29550"
     CDS             complement(<1..550)
                     /locus_tag="VF516_29550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111928.1"
                     /translation="MVGCATTNTELGSEHLGVAELHVDVGPLLATAITRVSVEAAGQT
                     QDLTFDFNTGSFDGVVFLPAGMQSLVARAFGGDMLVGQSHPTPVQVTPGATTRVLLRI
                     LDVSGAPGEVFGPILDSLSFPTTTNAGTTVTFAVSAVAPHGDPVTYAWSADCMDSTFT
                     ASTAATTGWSKPAQGVCTITVAA"
     gene            complement(760..1413)
                     /locus_tag="VF516_29555"
     CDS             complement(760..1413)
                     /locus_tag="VF516_29555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEX8111929.1"
                     /translation="MTTPELSGEPTPWSTADMARRAAREIPAGAIVNLGIGLPTQVGD
                     YLPDGHAWLHSENGLLGIGPFPYEGDEDPNLINAGKQTITVVPGGATFDSASSFAMIR
                     GGHVDLAILGAMQVSATGDLANWAVPGGKVMGIGGAMDLASGCRHVIAMMQLVTKKNE
                     HKLVRACDYPLTAARVVSLVITDLGVFQPAGDGFRVVDLAPGVTRDYAQARTGAPLH"
     gene            complement(1410..2123)
                     /locus_tag="VF516_29560"
     CDS             complement(1410..2123)
                     /locus_tag="VF516_29560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEX8111930.1"
                     /translation="MTGRHADKVVASIAEALAPLRDGMTVLVGGFGLSGNPEALIRGV
                     IERGVRDLTIVSNNAGNLGKGLALWLKAGIVRKVICSYIGNNDDLHTRMASGEITVEI
                     TPQGTLAERMRAAGAGIPAFYTPTGVGTVVEHGKETKEFDGRRYLLERALHGDVALVR
                     AHTADRFGNLRFWRTARNFNPVMATAAKLTLVECDRLVELGAIDPDNVHLSGIHVHRI
                     VHVPEHENAFEYRTVRKRP"
     gene            complement(2120..3406)
                     /locus_tag="VF516_29565"
     CDS             complement(2120..3406)
                     /locus_tag="VF516_29565"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8111931.1"
                     /translation="MDYPFFFTWTTQSAVKPVELTGGEGAWFTTADGGRWLDLGSLSY
                     QVNAGHGRRRIVEAIKRQADQLCLSTAHAVFPAKTELAERLLALAGPGFTKVFFTLGG
                     AEANENAVKIARLVTGRYKLISRYRSYHGASMGALTLTGDWRRTLVEPGVAGVVHVQD
                     CYCDRCPFGQKVETCARECASHIGTTMKLEGSRSVAAVILEPVPGANGVLVPPAEYWP
                     MVRAACDADGALLIADEVLTGFGRTGRAFCHQRWNVTPDLITCAKGITSGYQPLGAVI
                     VHDRVARHFDANILACGLTYYAHPTACAAGVETLRVYEDEKLFDNAERLGPVLHGELV
                     RIQARLAAPSFVRSIGLLGALDFVDEPPAKAWSQLERELAARRISLHVEPRRGTCIFA
                     PPLCITEDELVRGMRAFGDAAVAAFGGKAGAAGGVA"
     gene            complement(3419..4660)
                     /locus_tag="VF516_29570"
     CDS             complement(3419..4660)
                     /locus_tag="VF516_29570"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family peptidase"
                     /protein_id="HEX8111932.1"
                     /translation="MRQLELPAGQPPRVWGQIHGETFRGEIKALAAIRIYLCTKIGGF
                     KSSAEVRVAASVHLPVLERYHRGLYDELIGIAEGAAVSPEDIVVANHYTDLRDLDPDP
                     DAWRPAPLADDPGAGVVGSRAAGDEAGDQDSRASTEPSGAGPRDATRGGGNGPALPGH
                     RGRERPLPEDGCSVIWAESPTGRILAQTWDMHATAIPYVMVLRVPESPDGPPATLLSV
                     TGCLGMAGMNTARVAVAINNLFSTDATLGVVWPAVVRRALHRTTAAEARDAIATSPIG
                     SGHHYFVADRAGAFAIEASGTRRKQVFAGGRAYCHTNHCLDGDVAARSKVPPTSTTYD
                     RMRWLEADLARAPIADLADAWARLGSEDGWPRSVCTNMATPESPHGAATCGAIAMNLD
                     TGELWAQQGLVHNVAAERWQV"
     gene            complement(4657..>5329)
                     /locus_tag="VF516_29575"
     CDS             complement(4657..>5329)
                     /locus_tag="VF516_29575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111933.1"
                     /translation="GTAATIAALRAAVAAAGGPPSVLGLVYEVVQERTGQVLPAEFVA
                     ALGALRKELDLPLIAVETATHTYRSGKGPFLSAAIGLVPDVLTWWGGGQTGYLHVAPR
                     WFVPGPLTLVSTWDGDELSLIRQHHQLRAARQLDVAGAAAALDQALDLAPRSVTSAGI
                     GAYRVIDAGAQASSLAAALAERGIVVRRFPNQHLGIIPALDQIEAAARALGAALREVG
                     YKEAR"
BASE COUNT          728 a         1886 c         1965 g          750 t
ORIGIN      
        1 tcgcagccac ggtgatcgtg cagacgccct gggccggctt ggaccacccg gtggtcgctg
       61 cggtcgacgc ggtgaaggtc gagtccatgc agtcggcgct ccacgcgtag gtcaccggat
      121 cgccgtgcgg cgcgaccgcc gagaccgcga acgtcacggt ggtgccggcg ttggtcgtgg
      181 tcggaaacga cagcgagtcc aggatcggtc cgaacacctc ccccggcgcg ccgctgacat
      241 caaggatccg cagcaggacc cgcgtggtcg cacccggcgt cacctggacc ggggtcggat
      301 ggctctgacc gaccagcatg tccccgccga acgccctggc gaccaggctc tgcatccccg
      361 ccggcaggaa caccacgccg tcgaagctgc cggtgttgaa gtcgaacgtc aagtcctggg
      421 tctggccggc ggcctcgacc gagacccgcg tgatcgccgt ggcgagcagc ggccccacat
      481 cgacgtgcag ctcggcgacg cccaggtgct cgctcccgag ctctgtattc gttgttgcac
      541 agcccaccat cagaacagcg acactcaggt tggatatcca cgcgcgcacg aaaatctcct
      601 tgcagaggtc tgccgtgtcc gtctctgccc tcccgcagcg gacgcggcgc gctaacaacg
      661 cacgaccagc gcgatccagt ctcgcgatcg cgctggcaaa cctcagcaca ttcagcacat
      721 ccggaatgcc ggtctcggag ccggtaccgg ccggagcgct cagtgcagcg gcgcgccggt
      781 ccgggcctgg gcgtagtcgc gggtcacgcc gggggcgaga tcgacgacgc ggaacccgtc
      841 gccggccggc tggaacacgc cgagatcggt gatcacgagc gagaccacgc gcgccgcggt
      901 gagcgggtag tcgcaggcgc ggaccagctt gtgctcgttc ttcttggtca cgagctgcat
      961 catcgcgatc acgtggcggc agcccgaggc caggtccatc gcgccgccga tgcccatgac
     1021 cttgccgccg gggaccgccc agttggcgag gtcaccggtg gccgacacct gcatcgcgcc
     1081 gaggatcgcg aggtcgacgt ggccgccgcg gatcatcgcg aacgacgacg cggagtcgaa
     1141 cgtggcgccg ccggggacga cggtgatggt ctgcttgccg gcgttgatca ggttggggtc
     1201 ctcgtcgccc tcgtagggga acgggccgat gccgagcagg ccgttctcgg agtgcagcca
     1261 ggcgtggccg tcggggaggt agtcgccgac ctgggtcggc aggccgatgc cgaggttgac
     1321 gatcgcgccc gcggggatct cgcgcgccgc gcggcgggcc atgtccgcgg tcgaccaggg
     1381 tgtgggctcg ccagagagct cgggagtcgt catgggcgct tcctcacggt ccggtactcg
     1441 aacgcgttct cgtgctcggg gacgtggacg atgcggtgga catggatgcc ggacaggtgg
     1501 acgttgtcgg gatcgatcgc gccgagctcg accaggcgat cgcactcgac gagggtcagc
     1561 ttcgcggcgg tcgccatcac cgggttgaag ttgcgcgcgg tgcgccagaa ccggaggttg
     1621 ccgaaccggt cggcggtgtg ggcgcggacc agcgcgacgt cgccgtgcag cgcgcgctcg
     1681 agcaggtagc gccggccgtc gaactccttg gtctccttgc cgtgctcgac gacggtgccg
     1741 acgccggtcg gggtgtagaa cgccgggatg ccggcgccgg cggcgcgcat ccgctcggcg
     1801 agcgtgccct gcggggtgat ctcgacggtg atctcgccgg acgccatccg ggtgtggagg
     1861 tcgtcgttgt tgccgatgta gctgcagatc accttgcgca cgatgccggc cttgagccac
     1921 agcgccaggc ccttgccgag gttgccggcg ttgttggaca cgatggtcag atcgcgcacg
     1981 ccgcgctcga tcacgccgcg gatcagcgcc tcggggttgc cggacaggcc gaagccgccg
     2041 accaggacgg tcatgccgtc gcgcagcggc gcgagcgcct cggcgatcga ggcgacgacc
     2101 ttgtcggcgt gccggccggt catgccacac ctccggcggc acccgccttg ccgccgaacg
     2161 cggcgaccgc ggcgtcgccg aacgcgcgca tgccgcgcac gagctcgtcc tcggtgatgc
     2221 acagcggcgg cgcgaagatg caggtgccgc gccggggctc gacgtgcagc gagatccggc
     2281 gcgccgcgag ctcgcgctcg agctggctcc acgccttcgc gggaggctcg tcgacgaaat
     2341 cgagcgcgcc gagcaggccg atcgagcgca cgaagctcgg cgcggcgagc cgggcctgga
     2401 tccgcaccag ctcgccgtgc agcaccggcc cgagccgctc ggcgttgtcg aacagcttct
     2461 cgtcctcgta cacccggagg gtctcgacgc cggcggcgca cgccgtcggg tgcgcgtagt
     2521 aggtcagccc gcaggccagg atgttggcgt cgaagtggcg ggcgacgcgg tcgtgcacga
     2581 tcaccgcgcc gagcggctgg tagcccgagg tgatgccctt ggcgcacgtg atgaggtcgg
     2641 gggtgacgtt ccagcgctgg tggcagaacg cgcggccggt gcggccgaag ccggtcagca
     2701 cctcgtcggc gatcagcagc gcgccgtcgg cgtcgcacgc ggcgcgcacc atcggccagt
     2761 actcggcggg cggcaccagc acgccgttgg cgcccggcac cggctcgagg atcacggcgg
     2821 cgaccgagcg gctgccctcg agcttcatcg tcgtgccgat gtggctcgcg cactcgcggg
     2881 cgcaggtctc gaccttctgg ccgaacgggc agcggtcgca gtagcagtcc tggacgtgga
     2941 ccacgccggc gacgccgggc tcgaccagcg tgcgccgcca gtctccggtc agggtcagcg
     3001 cgcccatcga cgcgccgtgg tagctgcggt accggctgat cagcttgtac cgcccggtga
     3061 ccagccgcgc gatcttgacc gcgttctcgt tggcctcggc gccgcccagc gtgaagaaca
     3121 ccttggtgaa ccccggcccg gcgagcgcca gcagccgctc ggcgagttcg gtcttggccg
     3181 ggaacaccgc gtgggcggtc gacaggcaca gctggtcggc ctggcgcttg atcgcctcga
     3241 cgatccggcg ccggccgtgg cccgcgttga cctggtagga cagcgagccg aggtcgagcc
     3301 agcgcccgcc gtcggcggtg gtgaaccagg cgccctcgcc gccggtcagc tcgaccggct
     3361 tgacggcgct ctgggtcgtc caggtgaaga agaacgggta gtccatggcg ctcctagttc
     3421 atacctgcca gcgttcggcc gcgacattgt gcaccaggcc ctgctgcgcc cacagctcgc
     3481 cggtgtcgag gttcatcgcg atcgcgccgc aggtcgcggc gccgtgcggc gactccgggg
     3541 tcgccatgtt ggtgcacacg ctgcgcggcc agccgtcctc gctgccgagc ctggcccacg
     3601 cgtcggcgag atcggcgatc ggagcgcgcg cgaggtcggc ctccagccag cgcatccggt
     3661 cgtaggtcgt gctggtcggc ggcaccttgc tgcgcgcggc gacgtcgccg tcgaggcagt
     3721 ggttggtgtg gcagtacgcg cggccgccgg cgaacacctg cttgcgccgg gtgccggacg
     3781 cctcgatcgc gaacgcgccc gcgcggtcgg cgacgaagta gtggtggccc gagccgatcg
     3841 ggctggtcgc gatcgcgtcg cgcgcctcgg ccgcggtcgt gcggtgcagc gcccggcgga
     3901 ccaccgcggg ccacaccacg ccgagcgtgg catcggtgga gaacaggttg ttgatcgcga
     3961 ccgcgacccg cgccgtgttc atcccggcca tcccgaggca gccggtcacg ctgagcaggg
     4021 tcgccggcgg accgtcgggc gactccggca cgcgcagcac catcacgtac gggatcgcgg
     4081 tcgcgtgcat gtcccaggtc tgggcgagga tccggccggt cggcgactcg gcccagatca
     4141 cgctgcagcc gtcttcaggt aggggacgtt cccgtcccct gtggcccggc aaagccgggc
     4201 cattcccccc tccgcgcgtc gcgtcgcgcg gccctgcacc tgacggctcc gtgctcgccc
     4261 gggagtcctg gtccccggct tcatctccag cggcacgcga tccgaccacc ccggcgcccg
     4321 ggtcgtcggc gagcggcgcg ggccgccagg catcgggatc cggatcgagg tcgcgcaggt
     4381 cggtgtagtg gttggcgacc acgatgtcct cgggcgacac cgccgcgccc tcggcgatgc
     4441 cgatcagctc gtcgtacagg ccgcggtggt agcgctcgag caccggcagg tgcaccgacg
     4501 ccgcgacccg gacctcggcg gacgacttga acccgccgat cttggtacac aggtagatcc
     4561 ggatcgccgc gagcgccttg atctcgccgc ggaacgtctc gccgtggatc tgaccccaca
     4621 cgcgcggcgg ctggcccgcc ggcagctcga gctgtctcat cgcgcctcct tgtaccctac
     4681 ctcgcgcagc gcagcgccca gcgcgcgggc ggccgcctcg atctggtcga gcgcggggat
     4741 gatgccgagg tgctggttgg ggaaccggcg gaccacgatg ccgcgctcgg cgagcgccgc
     4801 cgcgagcgag ctcgcctgcg cgccggcgtc gatcacgcgg tacgcgccga tcccggccga
     4861 ggtcaccgac cggggagcaa gatcgagcgc ctggtcgagc gccgccgcgg cgccggcgac
     4921 gtcgagctgg cgcgccgcgc gcagctggtg gtgctggcgg atcagcgaca gctcgtcgcc
     4981 gtcccaggtc gacaccaggg tcagcggccc cggcacgaac cagcgcgggg cgacgtgcag
     5041 atacccggtc tggccgccgc cccaccaggt cagcacgtcg gggaccaggc cgatcgcggc
     5101 cgacaggaac ggtcccttgc cgctgcggta ggtgtgggtc gcggtctcga ccgcgatcag
     5161 cggcagatcg agctccttgc gcagggcgcc gagcgcggcg acgaactcgg ccggcagcac
     5221 ctggccggtg cgctcctgga cgacctcgta gaccaggccg agcacgctgg gcgggccgcc
     5281 ggcggcggcg accgcggcgc gcagcgcggc gatcgtcgcg gcggtcccg
//
LOCUS       DASVOR010001571         3065 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_454222,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001571 DASVOR010000000
VERSION     DASVOR010001571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3065
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454222"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..996)
                     /locus_tag="VF516_29580"
     CDS             complement(31..996)
                     /locus_tag="VF516_29580"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDP-sugar synthase"
                     /protein_id="HEX8111934.1"
                     /translation="MLLCAGLSTRLGRLGSERPKPLLPVCGVPILAYGITNLVAHGIT
                     ELVINTHHRPQMFEREIGDGRRFGAQIRYLHEPAILGTGGGLKHALPLLDPDGRDEPF
                     LSINGKLIFDLDITALVAAYQAQRAAGELLGMMVVRRVPDARDWGAVCVRGDDRGPYV
                     ADILGDGDHMFCGVHVTRPSVMARLPDGEADSIRQGYLPWLRAGERVAAYEHTGGYFA
                     EHSTPERYLASNWALLGGAPLRHPPTQLAGIDPTARLHPDAIIVPPVKIGAGAVVGPG
                     VTVGPDAVIGDGAVVRASIARTVVWAGAVVDAPPVEPVVTGEPAA"
     gene            1177..1425
                     /locus_tag="VF516_29585"
     CDS             1177..1425
                     /locus_tag="VF516_29585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111935.1"
                     /translation="MAAIADLVRPARAMQAPMICASQSRTPRDPCARAQPEANMTVRR
                     HVHHVHSGWPSPYIGVIAAILFGAAVLGVFSMLLWITR"
     gene            complement(1444..1803)
                     /locus_tag="VF516_29590"
     CDS             complement(1444..1803)
                     /locus_tag="VF516_29590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111936.1"
                     /translation="MTRRASWLAALALCAACKQPSDAPAPAPAPSMPATEIKRAQDAC
                     KAYVDKVCGCARTMPALEQPCRLARALPDAVQVGAEVGASADTAQRDVRQVQDTVRKI
                     ARQCIEELARLPAAGCS"
     gene            complement(1800..2591)
                     /locus_tag="VF516_29595"
     CDS             complement(1800..2591)
                     /locus_tag="VF516_29595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEX8111937.1"
                     /translation="MSTSFPLDGARVRFAGATNIGRKRDHNEDSISLPDSERLVIVAD
                     GMGGHASGEVASRLAVELITDHFNETGKQQTLTWPYKVDRDLRTHVNRMVTGIMLANL
                     EIWERSQKEARFKGMGTTCVAVYFLDDSLIIGHVGDSRCYRARGTELTQLTEDHSLIN
                     DYIRMKRVTPEEAENWPHKNVIVRALGMKESVQVDILTEKPHIGDTYLLCSDGLTGMV
                     KDDQMLHILMVERDLDRAVDRLIAAANEEGGIDNISVVLARVEAA"
     gene            2840..>3065
                     /locus_tag="VF516_29600"
     CDS             2840..>3065
                     /locus_tag="VF516_29600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111938.1"
                     /translation="MAELARGRVADRPWGRTFAALGLRGLTGQLSVLADGKRFLVGFD
                     HGAVVAAASPLASDAAARVALTIHLVSSSQV"
BASE COUNT          404 a         1083 c         1111 g          467 t
ORIGIN      
        1 ccatagcagc cgcctgcggc tgtccgtgcg tcaggcggcc ggctcgccgg tgaccaccgg
       61 ctcgaccggc ggcgcatcga cgacggcgcc ggcccacacc accgtgcggg cgatcgacgc
      121 gcgcaccacc gcgccgtcgc cgatcaccgc atcgggaccg acggtgacgc ccggcccgac
      181 gaccgcgccc gccccgatct tgaccggcgg gacgatgatc gcgtcgggat gcagccgtgc
      241 ggtcgggtcg ataccggcga gctgggtcgg cgggtggcgc agcggcgcgc cgccgagcag
      301 cgcccagttc gacgcgaggt agcgctccgg cgtggagtgc tcggcgaagt agccgccggt
      361 gtgctcgtag gcggcgaccc gctcacccgc gcgtagccac ggcaggtagc cctggcggat
      421 cgagtcggcc tcgccgtcgg gcaggcgcgc catcaccgac ggccgcgtca cgtggacgcc
      481 gcagaacatg tggtcgccgt cgccgaggat gtcggcgacg tagggcccgc ggtcgtcgcc
      541 ccggacgcag accgcgcccc agtcgcgggc gtcggggacg cgccgcacga ccatcatgcc
      601 cagcagctcg ccggcggcgc gctgcgcctg gtacgcggcg accagcgccg tgatgtccag
      661 gtcgaagatc agcttgccgt tgatcgacag gaacggctcg tcgcggccgt cgggatcgag
      721 cagcggcaag gcatgcttga ggccgccgcc ggtcccgagg atcgccggct cgtggaggta
      781 gcggatctgc gcgccgaacc gccggccgtc gccgatctcg cgctcgaaca tctgcggtcg
      841 gtggtgggtg ttgatcacga gctcggtgat gccgtgggcg accaggttgg tgatgccgta
      901 ggccaggatc ggcacgccgc acaccggcaa cagaggcttg ggccgctccg agcccagccg
      961 gccgagccgc gtgctcagcc cggcgcataa cagcatcgcc cgcatcgccc ccgacggtgg
     1021 cacggccgca cgcgggtggg caagcgaacg gacctcgcgg ccggctgcca cggggatgac
     1081 accgcgccgt caccgccgcg tcggtctcgg gaaatggcgc acagctcgcg tctgatgccc
     1141 gcagtgcgcg caacgcctgc aggagtcgac gcagccgtgg cagcaatcgc cgatcttgtg
     1201 cgcccggcgc gggccatgca ggcacccatg atctgtgcga gccagtcgcg aacgcctcgc
     1261 gatccgtgcg ctcgcgctca gccggaggcc aacatgaccg tacgccgcca cgtccaccat
     1321 gtccacagcg gctggccgtc tccgtatatc ggcgtgatcg cggccatcct gttcggggcc
     1381 gccgtcctgg gcgtcttcag catgctgctc tggatcaccc gttgagccgg cgcggtcgag
     1441 gcgttacgaa cagccggcgg cggggagcct ggcgagctcc tcgatgcact gcctggcgat
     1501 cttgcgcacg gtgtcctgga cctgccggac gtcgcgctgg gcggtgtccg cgctcgcgcc
     1561 gacctcggcg ccgacctgga ccgcgtcggg cagcgcgcgc gcgagccggc acggctgctc
     1621 gagcgccggc atcgtccggg cgcagccgca gaccttgtcg acgtaggcct tgcaggcatc
     1681 ctgggcgcgc ttgatctcgg tggccggcat cgagggggcg ggcgcgggcg cgggcgcgtc
     1741 cgacggctgc ttgcaggccg cgcacagcgc gagggcggcg agccaggacg cccggcgcgt
     1801 catgcggcct cgacccgggc gagcacgacc gagatgttgt cgatgccgcc ctcctcgttg
     1861 gccgccgcga tcaggcggtc gaccgcgcgg tcgaggtcgc gctcgaccat caggatgtgc
     1921 agcatctggt cgtccttgac catcccggtg agcccgtcgg agcacagcag gtaggtgtcg
     1981 ccgatgtgtg gcttctcggt caggatgtcg acctggacgc tctccttcat cccgagcgcc
     2041 cgcacgatca cgttcttgtg cggccagttc tcggcctcct cgggcgtcac ccgcttcatg
     2101 cggatgtagt cgttgatcag cgagtggtcc tcggtgagct gggtcagctc ggtgccgcgc
     2161 gcgcggtagc accggctgtc tcccacatgt ccgatgatca gcgagtcgtc gaggaagtac
     2221 accgcgacgc aggtcgtccc catgcccttg aaccgcgcct ccttctgcga ccgctcccag
     2281 atctccaggt tggcgagcat gatgccggtg accatccggt tgacgtgggt gcgcaggtcg
     2341 cggtcgacct tgtagggcca ggtcagcgtc tgctgcttgc cggtctcgtt gaagtggtcg
     2401 gtgatcagct cgaccgcgag ccggctcgcg acctcgcccg acgcgtggcc gcccatgcca
     2461 tcggcgacga tcaccagccg ctcgctgtcc ggcaacgaga tcgagtcctc gttgtgatcg
     2521 cgcttgcgcc cgatgttggt cgcgccggca aaccgcactc gtgctccgtc gagcggaaac
     2581 gacgtgctca tccgatgcca ctgtagcagg ggccaggcca gcggatcgat ccgtgccgcg
     2641 gcaaactgtc cgcccggccg tggcccgggc gcccgggctg acccgttgac tccaaccggc
     2701 gggccttaca tccttctgta gatctccatg tggggctatg cccagggtaa cgcgaccatc
     2761 gtcggcatcg tcgtcgtgat catcggtgcc gcagcgatcg gctacgtcgc gcggcggctg
     2821 tggcgaagag gcaggtgacg tggccgagct ggcgcgcgga cgggtcgcag atcggccgtg
     2881 ggggcggacc ttcgcggcgc tggggctgcg cggcctgacc gggcagctca gcgtgctggc
     2941 cgacggcaag cgcttcctgg tcgggttcga ccacggcgcg gtggtcgcgg cggcctcgcc
     3001 gctggcctcg gacgcggccg cgcgggtcgc gctgaccatc cacctggtgt cgtcgtcgca
     3061 ggtcg
//
LOCUS       DASVOR010001572         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_104565,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001572 DASVOR010000000
VERSION     DASVOR010001572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104565"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1877
                     /locus_tag="VF516_29605"
     CDS             <1..1877
                     /locus_tag="VF516_29605"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX8111939.1"
                     /translation="AVGARLYIEHPELVRARLDTLRFDIEWMGLPASLKNHYVNYPGI
                     TGSDVFETHVALIDRNVELGLGDPSLFVEEQVDDNPTRTKTTSVLSIASVPAAIQAST
                     PGFAYVGRSDLAPAGDIRLASRHLRWELTPVDFGHPVHVSLAATKARELAVAIANKTA
                     SSPAIVASAYRIEPPYTPTIKSLRVAYTTSVELDPTAAPTEHQALHVHPFGVCPIDRE
                     LPALLPRYESAGELYIGIRDLRPPQHLTLLLQLAEGTSDPDVEPAGLTWSYLDGDRFQ
                     DLAGSGIVDEPTRGLIHSGIVELALPAVAPSTRLPPELYWLRIALPRNPTSVCDTVAI
                     RAQAVAVRFEDRGNAAAHYQQPLPVGSIKRLVRANPAIARIEQPYSSFGGRPAEPPEL
                     FDTRVSERLRHKNRALSAWDYERLVLHGFRQIYRAKCLATGDGIDVVVIPDIRELHPS
                     DTFAPRAPANLLTDIEAYLVARAPAAARIRVRNARYVAVQVRLGVRFRPGIDPGFAQR
                     TLNDDLVRFLSPWAFDDGAELMIGGRIYASSIVDFVDRRDDIDFVADIKLFRSTDGKN
                     YDLVPPVVGEYHVATDRPDQVLVAAPQHYIDIIPEAGFKPTSFLGIDYARIQLDFIVG
                     "
     gene            1887..3077
                     /locus_tag="VF516_29610"
     CDS             1887..3077
                     /locus_tag="VF516_29610"
                     /inference="COORDINATES: protein motif:HMM:NF025253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HEX8111940.1"
                     /translation="MTIKNRTDLKSYFVKNAIPTEGNFADLIDSQLNQSQDGVFKPDG
                     EALSVVAATGDQKRALRLYASYPAPNPDWMIALNPAQDPATAGTSRAGLGVTDGAGRT
                     RLFIDAATGRIGVGTNNPQVALDVVGGVQANGFRGRYDLVLNDYRTVNPASNVCLQSP
                     GNDRDAWIYRDAADASRNWGIYHRQIDSPVAGLPANSIGFIGGDSSRLQAYVNLGDGS
                     GFFAGALSTPAGLIFEGSVPHIDRDGALYRNTDGQCYLTVDDHFYLRHSGTGANWAAH
                     FDTNDGNLELKGFLVASKLWSSDGHVQGADGSLMTDIANLSEPLNKLRALRGVTYRRR
                     DLLDGKARLGLLANEVAGMFPELVFTGRSGERGIEYAGLVALLIEAVKELHDFVINRK
                     PVPG"
     gene            3077..>3538
                     /locus_tag="VF516_29615"
     CDS             3077..>3538
                     /locus_tag="VF516_29615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111941.1"
                     /translation="MNDFPTLPIGPLPHIALDYARLRDEGLRLLGKLASAQWTDFNTH
                     DPGITILEQLCYAITDLGYRTSYPVPDLLAGGDPGLPPPEAILTTDPVTWADLRKVAL
                     DVDGIGNAWVEDAQPGPAFFHDAASGEIRLHAAPGDPTAAPAQVRGIHRVVL"
BASE COUNT          558 a         1336 c         1144 g          500 t
ORIGIN      
        1 ccgcggtggg cgcgcggctg tacatcgagc accccgagct ggtccgcgca cggctcgaca
       61 ccctgcggtt cgacatcgag tggatggggc tgcccgccag cctgaagaac cactacgtca
      121 actatcccgg gatcacgggc agcgacgtgt tcgagaccca cgtcgccctg atcgaccgca
      181 acgtcgagct cgggctcggc gacccctcgc tgttcgtcga ggagcaggtc gacgacaacc
      241 cgacccggac gaagaccacg tccgtgctct cgatcgccag cgtccctgct gcgatccagg
      301 ccagcacgcc gggcttcgcg tacgtcggcc gcagcgatct ggcgcccgcc ggcgacatcc
      361 ggctggccag ccggcacctg cgctgggagc tcacgccggt ggacttcggc cacccggtcc
      421 acgtatcgct cgccgcgacc aaggcccgcg agctcgcggt cgcgatcgcc aacaagaccg
      481 cgagcagccc ggcgatcgtt gcgtcggcct accgcatcga gccgccctac acccccacga
      541 tcaagtcgct gcgcgtcgcc tacacgacct cggtcgagct cgatcccacg gccgcgccca
      601 ccgagcacca ggccctccac gtccacccgt tcggcgtgtg cccgatcgac cgcgagctcc
      661 ccgccctgct gccgcgctac gagagcgcgg gcgagctcta catcggcatc cgcgacctgc
      721 ggccgccgca gcacctcacg ctgctgctgc agctcgccga ggggacctcc gatccggacg
      781 tcgagcccgc cgggctcacc tggtcctacc tcgacggcga ccggttccag gacctcgccg
      841 gctccgggat cgtcgacgag ccgacccgcg gcctgatcca ctcgggcatc gtcgagctgg
      901 cgctccccgc ggtggcgccg agcacccggc tcccgcccga gctgtactgg ctgcggatcg
      961 cgctgccgcg caacccgacg agcgtgtgcg acaccgtcgc gatccgggcc caggcggtcg
     1021 cggtccggtt cgaggaccgc ggcaacgcgg ccgcgcacta ccagcaaccg ctgccggtcg
     1081 gctcgatcaa gcgcctggtc cgcgccaacc cggcgatcgc ccggatcgag cagccctaca
     1141 gctcgttcgg cggccggccg gccgagccac ccgagctgtt cgacacccgg gtgagcgagc
     1201 ggctgcgcca caagaaccgc gcgctgagcg cctgggacta cgagcgcctg gtgctgcacg
     1261 gcttccgcca gatctaccgg gccaagtgcc tggcgaccgg cgatggcatc gacgtggtcg
     1321 tcatcccgga catccgcgag ctccacccca gcgacacgtt cgcgccgcgc gcgccggcca
     1381 acctgctcac cgacatcgag gcctacctgg tcgcgcgggc gccggcggcg gcgcggatcc
     1441 gcgtccgcaa cgccaggtac gtggcggtcc aggtccggct cggcgtccgg ttccgccccg
     1501 ggatcgaccc ggggttcgcg cagcggacgc tgaacgacga cctcgtccgc ttcctgtcgc
     1561 cgtgggcgtt cgacgacggc gccgagctga tgatcggcgg gcggatctac gccagcagca
     1621 tcgtcgactt cgtcgaccgg cgcgacgaca tcgacttcgt cgccgacatc aagctgttcc
     1681 gcagcacgga cggcaagaac tacgatctcg tcccgccggt ggtgggcgag taccacgtcg
     1741 cgaccgaccg gccggatcag gtgctggtcg cggcaccgca gcactacatc gacatcatcc
     1801 cggaggccgg gttcaagccg accagcttcc tcgggatcga ctacgccagg atccagctcg
     1861 atttcatcgt ggggtgaggg caggccatga caatcaagaa ccgcaccgat ctcaagtcgt
     1921 acttcgtcaa gaacgcgatc cccaccgagg ggaacttcgc cgatctgatc gactcgcagc
     1981 tcaaccagtc gcaggacggc gtgttcaagc ccgacggcga ggcgctcagc gtggtcgccg
     2041 cgaccggcga ccagaagcgc gcgctgcggc tgtacgccag ctaccccgcg cccaaccccg
     2101 actggatgat cgccctcaac cctgcccagg atccggcgac cgcggggacc agccgcgccg
     2161 gcctgggggt caccgacggc gccggacgga ccaggctgtt catcgacgcc gcgaccggcc
     2221 ggatcggcgt cgggaccaac aacccccagg tcgcgctcga cgtggtgggc ggcgtgcagg
     2281 ccaacgggtt tcgcggccgg tacgacctgg tgctcaacga ctaccggacg gtgaaccccg
     2341 cgtccaacgt ctgcctgcag tcaccgggca acgatcgcga tgcatggatc taccgcgatg
     2401 ccgccgacgc ctccaggaac tggggcatct accaccgcca gatcgactcg ccggtcgccg
     2461 gcctgcccgc caactcgatc gggttcatcg gcggcgacag ctccaggctc caggcctacg
     2521 tcaacctcgg cgacgggtcg ggcttcttcg ccggcgcgct cagtaccccc gcagggttga
     2581 tcttcgaggg cagcgtgccg cacatcgatc gagacggcgc gctgtaccgg aacaccgatg
     2641 gccagtgcta cctgaccgtc gacgatcact tctacctccg ccactccggc accggcgcga
     2701 actgggcggc gcacttcgac accaacgacg gcaacctgga attgaagggc ttcctggttg
     2761 cctcgaagct gtggtcgagc gacggccatg tccagggcgc ggatgggtcg ttgatgaccg
     2821 acatcgcaaa cctgtcggag ccgctgaaca agctccgcgc gctccgcggc gtcacgtatc
     2881 ggcgacgcga cctgctcgac ggcaaggccc gtctcggtct gctcgccaac gaggtcgcgg
     2941 gcatgttccc cgagctggtc ttcaccggtc gatccggcga gcggggaatt gagtacgccg
     3001 gattggtggc tctgctcatc gaggcggtca aggagctcca cgacttcgtc atcaaccgca
     3061 aaccggttcc agggtgatga acgacttccc gacgctgccg atcggcccgc tgccgcacat
     3121 cgcgctggac tacgcgcggc tgcgcgacga ggggttgcgc ctgctgggca agctcgccag
     3181 cgcgcagtgg accgacttca acacccacga cccggggatc acgatcctcg agcagctgtg
     3241 ctacgcgatc accgatctcg gctatcgcac cagctatccg gtgcccgatc tgctggcggg
     3301 cggcgacccc ggcctgccgc cgcccgaggc gatcctgacc accgatccgg tcacctgggc
     3361 ggacctccgc aaggtggcgc tcgacgtcga cggcatcggt aacgcctggg tcgaggatgc
     3421 ccagcccggg ccggcgttct tccacgacgc cgcgagcggc gagatccggc tgcacgccgc
     3481 tcccggcgac cccaccgccg cgccggcgca ggtccgcggc atccatcgcg tggtgctc
//
LOCUS       DASVOR010001573         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_45632,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001573 DASVOR010000000
VERSION     DASVOR010001573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45632"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..492
                     /locus_tag="VF516_29620"
     CDS             <1..492
                     /locus_tag="VF516_29620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111942.1"
                     /translation="DFEREDQGTTSGVVLFRHTTRSRPHLAVSVVMGPCSAQRACQAM
                     DLASWTARQGELRGQLPKELRSRPDTRFEIGVRAIAGAPAIYTYQLGYAGGVDEKDQP
                     SADYTDAYVLYYSDGINQIRVMAHYVDDALGGIPQLLAIAPPDDLEKLAVAFASFYLH
                     AWQ"
     gene            complement(507..956)
                     /locus_tag="VF516_29625"
     CDS             complement(507..956)
                     /locus_tag="VF516_29625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEX8111943.1"
                     /translation="MSLVARTNGNTPARYTARDPFQFARDLLSWDPFFAGTRQASAFV
                     PAFEVKETSEAFILKGDLPGVAESDLDIAVHNNVLTVSGSRQSEDRKEGESYALYERQ
                     YGSFSRSFSLPDTADGERVEAKLDAGVLTLTIWKRAEAKPRKIALKK"
     gene            complement(1170..2090)
                     /locus_tag="VF516_29630"
     CDS             complement(1170..2090)
                     /locus_tag="VF516_29630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111944.1"
                     /translation="MLFPAPPAPVFPPDQATTVDHHPRYEDCTQDGRLNTLAIPPSLA
                     STWNAREARYPAIRQARAKGLVSLLTRLTVVSFDAPVRVNRPFEVTGGFQLAHDRDAS
                     GAVSKLFMNVWTQIRGRPHLPREVWDAARASGQSAQLVLAGQAFTEHTYTRPFGPPDQ
                     RSVTSLAGIEGVPEVPELRYHQPAPTSAAEAPDGARWLDELAADPVDTMFTLDQTDAN
                     QHVNSLIYVRVFLAAMQRRLASGGHPARLRSKGFDIAYRKPSFVGDRVRAHIRMFADG
                     DGTHIGGAGFLAADGEEARPRCYVRAVYGP"
BASE COUNT          322 a          714 c          793 g          323 t
ORIGIN      
        1 gacttcgagc gcgaggatca gggcacgacc tccggcgtgg tgctgttccg ccacaccacg
       61 cggagccggc cgcacctcgc ggtcagcgtc gtgatggggc cgtgcagcgc ccagcgcgcg
      121 tgccaggcga tggacctcgc gagctggacc gcgcggcagg gcgagctgcg cggccagctc
      181 ccgaaggagc tgcgcagccg ccccgacacc cggttcgaga tcggggtgcg cgcgatcgcc
      241 ggcgcaccgg cgatctacac gtaccagctc ggctacgcgg gcggtgtcga cgagaaggac
      301 cagccgtccg ccgactacac cgacgcgtac gtgctgtact acagtgacgg catcaaccag
      361 atccgcgtga tggcccacta cgtggacgac gcgctgggcg gcatcccgca gctcctggcc
      421 atcgctccgc cggacgatct ggagaagctc gcggtcgcgt tcgcgagctt ctacctgcac
      481 gcctggcagt gactacctgg cagtgactac ttcttcagtg cgatcttgcg cggcttggcc
      541 tcggccctct tccagatcgt cagcgtgagg acgccggcgt cgagcttggc ctcgacgcgc
      601 tcaccgtcgg ccgtgtcggg gagcgagaag ctgcgcgaga acgagccgta ctggcgctcg
      661 tagagcgcat agctctcgcc ctccttgcgg tcctcggact ggcggctgcc ggacaccgtc
      721 agcacgttgt tgtggacggc gatatcgaga tccgactcgg cgacgccggg gagatcgccc
      781 ttcaggatga aggcctcgct ggtctccttg acctcgaacg cggggacgaa cgcgctggcc
      841 tggcgggtgc cggcgaagaa cgggtcccag gacagcagat cacgggcgaa ctggaacggg
      901 tcacgggcgg tgtagcgggc gggggtgttg ccgttggtgc gagcgacgag cgacatggga
      961 aaaattcctc ctgtgcaggg aagatgggtt gttgaagtca cctccttgat aagcacgtcg
     1021 aggggtcacg caacggcttg acgcggattg ccgcgatggt taccgagccg gttccacctg
     1081 cccggtcgat cggagagggg gctttcccgc cccctctttc gcggcaaagc cgcgaaattc
     1141 acccctccgc gcgtcgcgtc gcgcggatct tacgggccat agacggcgcg gacatagcac
     1201 cggggccgcg cctcctcgcc gtcggctgcg agaaaccctg cccccccgat gtgggtgccg
     1261 tcgccgtccg cgaacatccg gatgtgggcg cggacccggt cgccgacgaa gctcggcttg
     1321 cggtacgcga tgtcgaagcc cttggagcgc agccgcgcgg ggtggccgcc ggacgcgagc
     1381 cggcgctgca tcgcggcgag gaacacgcgg acgtagatca gcgagttgac gtgctggttg
     1441 gcgtcggtct ggtcgagcgt gaacatcgtg tcgaccggat cggcggccag ctcgtcgagc
     1501 cagcgcgcgc cgtcgggcgc ctcggccgcc gaggtcggcg ccggctggtg gtagcgcagc
     1561 tccggcacct cgggaacgcc ttcgatgccg gccaggctgg tgacactgcg ctgatcgggc
     1621 ggcccgaacg ggcgggtgta ggtgtgctcg gtgaacgcct ggccggcgag cacgagctgc
     1681 gccgattgcc cggacgcgcg cgccgcgtcc cacacctcgc gcggcaggtg cgggcggccg
     1741 cggatctggg tccacacgtt catgaacagc ttggacaccg cgccgctcgc atcgcggtcg
     1801 tgcgcgagct ggaacccacc ggtgacctcg aacggccggt tgacccggac cggcgcgtcg
     1861 aacgagacga cggtcagccg ggtcagcagc gagaccagcc ccttcgcgcg ggcctggcgg
     1921 atcgcggggt agcgcgcctc gcgggcgttc caggtcgacg ccagcgaggg cgggatcgcc
     1981 agcgtgttga gccggccgtc ctgcgtgcag tcctcgtagc gcgggtggtg gtcgacggtg
     2041 gtcgcttggt ccggcgggaa caccggcgca ggaggtgcgg gaaacagcat cgcgcgcagc
     2101 ataggacaga ggtagggtgc tgcgtgccca ccgcgctgcc gatcgacgcc gt
//
LOCUS       DASVOR010001574         1831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_17073,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001574 DASVOR010000000
VERSION     DASVOR010001574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1831
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17073"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..219)
                     /locus_tag="VF516_29635"
     CDS             complement(<1..219)
                     /locus_tag="VF516_29635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111945.1"
                     /translation="MSIPTGRFVWFEYVSQDAKKAQGFFGELFGWSTKGVPMPDGEYT
                     MIAAPDGRTIGGYFAAPDPRTPAGWLPYL"
     gene            complement(445..933)
                     /gene="msrB"
                     /locus_tag="VF516_29640"
     CDS             complement(445..933)
                     /gene="msrB"
                     /locus_tag="VF516_29640"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEX8111946.1"
                     /translation="MTTPRTSTSQPPRSPGTSASGFDITPLSDDRVAALAAAFTPEER
                     HVLLHQGTERPFCGLLLKNKQPGTYTCRLCGLPLFRSDAKFESGTGWPSFFQPFDRDH
                     VTELTDHSHGMVRVEIRCKRCDAHLGHVFDDGPPPTHQRYCLNSVSLSFVPAGEPTPL
                     RS"
     gene            complement(1017..1544)
                     /locus_tag="VF516_29645"
     CDS             complement(1017..1544)
                     /locus_tag="VF516_29645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111947.1"
                     /translation="MMLKLLPLTALTLGLSSGVALADHHGGEYRGDHRVVIEHRGAPV
                     FRDHGREWHGGEWHGGAVVRGGGEWRGRTFVREGRDWHGGGRVVVREPYYRHDYRVVR
                     RPIFVSRPIIREHYYNHYRQPSLIVENYGAMPGYYWVAGQWSWNGYEWLWQPGHYEPD
                     PNYVDPAYADPGYGY"
BASE COUNT          290 a          588 c          685 g          268 t
ORIGIN      
        1 caggtagggc agccagccgg ccggggtcct gggatcgggc gcggcgaagt agccgccgat
       61 cgtcctgcca tcgggtgcgg cgatcatcgt gtactcaccg tcgggcatcg gcacgccctt
      121 ggtgctccag ccgaacagct cgccgaagaa gccctgcgcc ttcttggcgt cctgcgagac
      181 gtactcgaac caaacgaagc ggccggtggg aatcgacatg ggaatctcct gtctgaggtg
      241 ggtgcgttac gccaggaggt cgaacgatgg ccgtccagat cgacagcgca tatgcagcgc
      301 ggcgaaaaaa ccaccgcgcg accggcaagc acgaacgatc tcggtgggat ggtaccgcgg
      361 cggcgggatc gcgccagcgc gtcagcccgg tcgatcggtc cgccggagac tcccagaact
      421 ccggaccgag agatccggag cgcgtcagct gcgcagcggc gtcggctcgc ccgcgggtac
      481 gaagctcagc gagaccgagt tcaggcagta gcgctggtgg gtcggcggtg ggccgtcgtc
      541 gaacacatgg ccgaggtgcg cgtcgcagcg cttgcagcgg atctcgacgc gcaccatgcc
      601 gtggctgtga tcggtgagct cggtgacgtg gtcgcgatcg aacggctgga agaagctcgg
      661 ccagccggtg ccggactcga acttggcgtc cgaccggaac agcggcaggc cgcacagccg
      721 gcaggtgtac gtgccgggtt gcttgttctt gagcaacagg ccgcagaacg ggcgctcggt
      781 gccctggtgc aggaggacgt ggcgctcctc gggcgtgaac gcggcggcga gcgcggcgac
      841 gcggtcgtcg gacagcggcg tgatgtcgaa cccggacgcg gaggtgccgg ggctgcgggg
      901 cggctgcgag gtcgaggtcc gcggggtcgt catacgctca tgcatacgcc aaaacaccgg
      961 cggcggttac cccggtgtgg gcaaccgccg cggggtctgg ccgctgcggg agcgggctag
     1021 taaccgtagc cggggtcggc gtacgccgga tcgacgtagt tcggatccgg ctcgtagtga
     1081 cccggctgcc agagccactc gtagccgttc caggaccact ggccggcgac ccagtagtag
     1141 cccggcatcg cgccgtagtt ctcgacgatc agcgagggct gccggtagtg gttgtagtag
     1201 tgctcgcgga tgatcgggcg ggacacgaag atcgggcggc gcacgacgcg gtagtcgtgg
     1261 cggtagtacg gctcgcggac gaccacccgg ccgccgccgt gccagtcgcg gccttcgcgc
     1321 acgaaggtcc ggcctcgcca ctcgccgccg ccgcggacga cggcgccgcc gtgccattcg
     1381 ccgccgtgcc attcgcggcc gtggtcgcgg aacaccggag cgccccggtg ctcgatgacc
     1441 acgcgatggt cgccgcggta ttcgcctccg tgatggtcgg ccagggcgac accactcgag
     1501 aggccaagag tgagcgcggt cagggggaga agcttgagca tcatggcctc ggataaagca
     1561 cccgccgtgc cgcgctcgcg cccagcaatt tcaactggtt ctgggatcgt ttgccggtcg
     1621 gctgcagaac ccgcagttca cggtcctgcg gattccgcag tcggggcgga atgcctcggc
     1681 cctgcgtgcg acgcggaggc gaggtgaggg cgagcaggcg agctgcgccg acgacggaga
     1741 ccgacgcagc gcggccgctc cggctcacgc gtcgtcgtac ttcttgagcc ggtcgtacag
     1801 cgtgcgctcg ccgatcccga gcagctcggc g
//
LOCUS       DASVOR010001575         2954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_483812,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001575 DASVOR010000000
VERSION     DASVOR010001575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2954
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_483812"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2954
                     /locus_tag="VF516_29650"
     CDS             <1..>2954
                     /locus_tag="VF516_29650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8111948.1"
                     /translation="PGVAGTWKDLTDNVNSMASNLTNQVRNIADVTIAVANGDLSKTI
                     TVDVRGEILQLKEAINTMVDQLNGFASEVSRVAREVGTEGKLGGQAEVPGVAGTWTDL
                     TDNVNSMASNLTGQVRNIADVATAIARGDLSRKITVDVKGEILELKNTLNTMVDQLNA
                     FASEVSRVAREVGTEGKLGGQAVVTGVAGTWKDLTDTVNSMASNLTGQVRNIADVVTA
                     IARGDLSRKITVDVRGEILQLKEAINTMVEQLRSFASEVTRVAREVGTEGRLGVQAVV
                     PGVAGTWKDLTDSVNAMGTNLTAQVRNIAEVTTAVARGDLSRKITVDVKGEILELKNT
                     INTMVDQLRSFASEVTRVAREVGTEGKLGGQAEVQGVAGTWKDLTDSVNFMAANLTNQ
                     VRGIVRVVTAVANGNLRQKLTVEAKGEVAALAETINNMTDTLATFAEQVTNVAREVGV
                     DGRLGGQANVPGAAGTWKDLTGNVNLLAANLTNQVRAIADVATAVTKGDLTRSIQVEA
                     RGEVAELKDNINTMIDNLRATTDRNQEQDWLKTNLAKFTRMLQGQRELVTVGRMLLSE
                     LAPLVGAQHGTVYQLERPDPGEVSDTAPGPKLLRLLAAYARDDEQPERIALGVGLVGQ
                     AAAEKQRILLNDIPPDYTRIHSSLGSALPTSVVVLPVLFEGETMAVIELAALRPFTGT
                     HLAFLDQLTQSIGVVLNTIEATMRTENLLQQSQQLTAELQTRQIELQQTNEELASKAK
                     QLAEQNAEVERKNKEVEQARGALEEKAAELALTSKYKSEFLANMSHELRTPLNSILIL
                     GQQLAENVGGNLTTKQVDFARNIHSAGTDLLTLINDILDLSKIESGTVTVEPEEITFG
                     SLRDTVHRTFHHIAETRALAFNVEIDPTLPRTMTSDPKRLQQILKNLLSNAFKFTSQG
                     FVSMAVRHMTSGWTPDHPVLGTAGTAVAFMVTDTGIGIAPEKQKLIFEAFQQADAGTS
                     RKYGGTGLGLAI"
BASE COUNT          557 a         1084 c          945 g          368 t
ORIGIN      
        1 cccggcgtcg ccggcacctg gaaggacctg accgacaacg tcaactcgat ggcgtcgaac
       61 ctgaccaacc aggtccgcaa catcgccgac gtcaccatcg cggtggccaa tggcgacctg
      121 tccaagacga tcacggtcga cgtccgcggc gagatcctcc agctcaagga ggcgatcaac
      181 accatggtcg atcagctcaa cggcttcgcg tccgaggtgt cgcgcgtcgc ccgtgaggtc
      241 ggcaccgagg gcaagctcgg cggccaggcc gaggtccccg gcgtcgccgg cacctggacg
      301 gacctgaccg acaacgtcaa ctcgatggcg tcgaacctga ccggccaggt ccgcaacatc
      361 gccgacgtcg cgaccgcgat cgcgcgcggc gacctcagcc gcaagatcac ggtcgacgtc
      421 aagggcgaga tcctcgagct caagaacacg ctcaacacga tggtcgacca gctcaacgcg
      481 ttcgcctccg aggtgtcgcg cgtcgcgcgc gaggtcggca ccgagggcaa gctcggcggt
      541 caggcggtcg tgaccggcgt cgccggcacc tggaaggacc tcaccgacac cgtcaactcg
      601 atggcgtcga acctcaccgg ccaggtccgc aacatcgccg acgtcgtgac cgcgatcgcg
      661 cgcggcgacc tcagccgcaa gatcaccgtc gacgtccgcg gcgagatcct ccagctcaag
      721 gaggcgatca acaccatggt cgagcagctg cggtcgttcg cctccgaggt gacccgggtc
      781 gcccgcgagg tcggcaccga gggccggctc ggcgtccagg ccgtggtgcc cggcgtcgcc
      841 ggcacctgga aggacctcac cgactcggtc aacgcgatgg gcaccaacct gaccgcccag
      901 gtccgcaaca tcgccgaggt cacgaccgcg gtcgcgcgcg gcgatctcag ccgcaagatc
      961 accgtcgacg tcaagggcga gatcctcgag ctcaagaaca cgatcaacac catggtcgac
     1021 cagctgcggt cgttcgcgtc cgaggtcacc cgcgtcgccc gcgaggtcgg caccgagggc
     1081 aagctcggcg gccaggccga ggtccagggc gtcgccggca cctggaagga cctcaccgac
     1141 tcggtcaact tcatggccgc caacctgacc aaccaggtcc gcggcatcgt ccgcgtggtc
     1201 accgcggtcg ccaacggcaa cctgcgccag aagctcaccg tcgaggccaa gggcgaggtc
     1261 gccgcgctcg ccgagaccat caacaacatg accgacacgc tcgcgacgtt cgccgagcag
     1321 gtgaccaacg tcgcccgcga ggtcggcgtc gacggccggc tcggcggcca ggccaacgtg
     1381 cccggcgccg ccggcacctg gaaggacctg accggcaacg tcaacctgct cgccgccaac
     1441 ctgaccaacc aggtccgcgc gatcgccgac gtcgccaccg cggtcaccaa gggcgacctg
     1501 acccggtcga tccaggtcga ggcgcgcggc gaggtcgccg agctcaagga caacatcaac
     1561 acgatgatcg acaacctgcg cgcgacgacg gatcgcaacc aggagcagga ctggctcaag
     1621 accaacctcg cgaagttcac ccgcatgctg cagggccagc gcgagctggt gaccgtcggc
     1681 cggatgctgc tgtccgagct cgccccgctg gtcggcgcgc agcacggcac ggtgtaccag
     1741 ctcgagcgcc ccgatcccgg cgaggtcagc gacaccgcgc ccggccccaa gctattgcgc
     1801 ctgctcgccg cgtacgcgcg cgacgacgag cagcccgagc ggatcgcgct cggcgtcggg
     1861 ctggtcggcc aggccgcggc cgagaagcag cgcatcctgc tcaacgacat cccgccggac
     1921 tacacccgga tccactccag cctgggcagc gcgctgccga ccagcgtggt cgtgctgccg
     1981 gtgctgttcg agggtgagac catggcggtg atcgagctcg ccgcgctgcg cccgttcacc
     2041 ggcacccacc tcgcgttcct cgatcagctg acccagtcga tcggcgtcgt gctcaacacg
     2101 atcgaggcga cgatgcggac cgagaacctc ttgcagcaga gccagcagct gaccgccgag
     2161 ctccagaccc ggcagatcga gctccagcag accaacgagg agctcgcgtc caaggccaag
     2221 cagctcgccg agcagaacgc cgaggtcgag cgcaagaaca aggaggtcga gcaggcgcgc
     2281 ggcgcgctgg aggagaaggc ggccgagctc gcgctgacct cgaagtacaa gtccgagttc
     2341 ctcgccaaca tgtcgcacga gctgcgcacg ccgctcaact cgatcctgat cctcggccag
     2401 cagctggccg agaacgtcgg cggcaacctg acgaccaagc aggtcgactt cgcccggaac
     2461 atccactcgg ccggcaccga cctgttgacc ctgatcaacg acatcctcga cctgtcgaag
     2521 atcgagtcgg gcaccgtgac ggtcgagccc gaggagatca cgttcggctc gctgcgcgac
     2581 accgtgcacc gcacgttcca ccacatcgcc gagacccgcg cgctggcgtt caacgtcgag
     2641 atcgatccga cgctgccgcg cacgatgacc agcgacccca agcgcctgca gcagatcctc
     2701 aagaacctgc tgtccaacgc gttcaagttc acgtcgcagg gcttcgtgtc gatggcggtc
     2761 cgccacatga cgtcgggctg gacgccggac cacccggtgc tcggcaccgc cgggaccgcg
     2821 gtcgcgttca tggtgaccga caccggcatc ggcatcgcgc ccgagaagca gaagctgatc
     2881 ttcgaggcgt tccagcaggc cgacgccggc accagccgca agtacggcgg caccggcctg
     2941 ggcctggcga tcag
//
LOCUS       DASVOR010001576         6318 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_45652,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001576 DASVOR010000000
VERSION     DASVOR010001576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6318)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6318)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6318
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45652"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..790
                     /locus_tag="VF516_29655"
                     /pseudo
     CDS             62..790
                     /locus_tag="VF516_29655"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
     gene            950..1258
                     /locus_tag="VF516_29660"
     CDS             950..1258
                     /locus_tag="VF516_29660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEX8111949.1"
                     /translation="MPTYEYRCSACHREFEYQQKMSDADLVHCEACGQDKLERLISWT
                     SVRSTGGIRPVHYSAQGIQGLPPADRSRGPQRFTSAEPAAPCGDACACAPATGGTDSE
                     "
     gene            complement(1266..2015)
                     /locus_tag="VF516_29665"
     CDS             complement(1266..2015)
                     /locus_tag="VF516_29665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111950.1"
                     /translation="MKLAQIALAVALCSGCSAQISDGRDPSLGGITTPDAGAGGGPGP
                     TGDAGLTTCTRRTLYLNFDGQTLTQGPSDATQNTAQWMNKMNGAAPPYRQTDTGRDLQ
                     IQTIVDGVTRQLARFPIAVTRIRPSSGNYVMIVYGGTASMVGSNFGAAVNQLDCGDTR
                     PDDVAWIADNVTGQRAINTTIGAIGFGLGLTATIDPADCMCSWDNGCLPDNTVRCTLG
                     SPIARDPQANQRCAGTTSQDEAAAFRTAFCQ"
     gene            2204..3439
                     /locus_tag="VF516_29670"
     CDS             2204..3439
                     /locus_tag="VF516_29670"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEX8111951.1"
                     /translation="MARINEHYAKLPASYLFPEIGRRVRAYQAAHPDAKVIRLGIGDV
                     TEPLAPAIVEAMHAAVDEMGRRETFRGYGPEQGYDFLIDAIREHDYAARGVTIGADEV
                     FVSDGSKCDSGNIQEIFALAARIAVTDPVYPVYVDSNVMAGRTGRIGADGRYAGLTYL
                     VGNEANGFQPEPPAEPVDVVYLCSPNNPTGTVATRAQLERWVSWARANDAIILFDAAY
                     EGYIADPALPHSIYEVDGAKDCALELRSFSKRAGFTGVRCAFLVVPKTVTGTDPDGKR
                     VSLNALWSRRHSTKFNGASYVVQRGAQAAYSPAGLEQTRAQIAFYMETARLLREGLVA
                     AGFTVFGGVHAPYLWLRTRDGASSWAFFDRLLAEVQIVGTPGAGFGPAGEGYFRLSAF
                     NSRANIEEAIARIRRGFGR"
     gene            3444..4232
                     /locus_tag="VF516_29675"
     CDS             3444..4232
                     /locus_tag="VF516_29675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8111952.1"
                     /translation="MRWSLEAAGHAERAPAHAMMVRRSGSGPELVWIHGLGEQSRSLD
                     AIAAHPALAGFSHVLVDLPGYGRSPWPEHPDDLEQLADRLAGWIGDRRPALIGHSMGG
                     VLATLIAERIAVRAVIDVEGNLSRGDCTHSALAAAYALDDFLAHGYATMRAEVYEGGR
                     TDLALRGYHAAMTMASPRVFHHHALQLVAMSEPETLVTRLAALRAPVRFIAGGLPGGI
                     CERSRALLDRHAVRWVGIEPAGHWVHIDQPDRFAAEVAGFLATL"
     gene            complement(4244..4456)
                     /locus_tag="VF516_29680"
     CDS             complement(4244..4456)
                     /locus_tag="VF516_29680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111953.1"
                     /translation="MEFQRLREAWERAHPRRATGGVVSLRGFLYQFQAVVRAWVADPT
                     SVEVVVESVSDRLELAHMVDEGAVPE"
     gene            complement(4537..4902)
                     /locus_tag="VF516_29685"
     CDS             complement(4537..4902)
                     /locus_tag="VF516_29685"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8111954.1"
                     /translation="MGIRIPAKIARKIEAAGRDNELFISAITPWEIALGVRRARIKIA
                     GDVLEWIHGALDALSATVASLEPAIAVDAVDLAWDHADPSDRMIVATARRLGASLVTA
                     DTAILEFASSTKAVRVLEL"
     gene            complement(4928..5161)
                     /locus_tag="VF516_29690"
     CDS             complement(4928..5161)
                     /locus_tag="VF516_29690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEX8111955.1"
                     /translation="MKISAVKFKATCLKLIDRVAETGEPVVITKRGKVMARLVPDHGT
                     PRGLGKGRGSIEILCTDEELFQLGAGEPWDAGA"
     gene            complement(5230..6183)
                     /locus_tag="VF516_29695"
     CDS             complement(5230..6183)
                     /locus_tag="VF516_29695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111956.1"
                     /translation="MLALAIVLHAGAARAQRRGYIDGFVGWSQDGSFFVMTTAGTDEM
                     DVPVLCLSRRGVPSPTWPKDVPVPDPDDPDGCTDRWDMMFPDNTLDAQHMVLKARQLV
                     ATPRHNGKGPHGEDYDLRRVRDGVIEVAISRGGKRVARGFFELRLPKDPVPDAYEAFW
                     RGDGRAVAIAAGYVPAKDPGPGFGPPRYLVVIPLDGSTANAQAPPSHRQRAQALNVEG
                     LKLLAAKNLDAAQQKFQAATQEDDSFALAYYNLACAASLRKDKQTALGALHHLSAVED
                     EDPVAKKSLAKGATDHDLDFIASDPEGAKLLKRGSPPKRMP"
BASE COUNT          887 a         2224 c         2245 g          962 t
ORIGIN      
        1 cccgacgcgc ggcagagggc agctccacat aatcggcggc tccgcataag agcaccagag
       61 caccaccgat ccggggatgc cgctgcgcac gctgtcctat cgcgtccgga tctggaatcg
      121 ctgtcgcaag cagcgccccg aggcgtggct cccgccaatc ctcacggtgc tcgtcagcca
      181 ggtgccgggt ggctggacca cctcgtgcct gctcgacgac atgttcgatc cgcgcgtgcg
      241 ggcgatcccg gagctggcgg cgtcgatccc gcgatcttcg gtgttcatcg acgacctcgc
      301 ccggctgtcc gacgccgacc tccaggcgcg ctcgctggct gcgttccaga agcttgcgct
      361 ctggctgctg cgcgacgctc gcgctcccag gcgcctgctc gacagcttcg acacctggat
      421 cgatgctttc gccgaggccg agcgagcgcc cacgggcgtc gacgcgatcg ccacgctgct
      481 gacgtatctg ttccgggtga tcgatccgat gcaccgcggg gagctccgtg gcaagatccg
      541 tctactggga cccagggccg aggagactgc catgaccatt gccgaacagc tacatgagga
      601 agggctcttg aagggccgcg aggaagggcg gatcgctgcg ctgcgctccg tgctcgtctc
      661 ccggttcggg tcccaggcgc tggacgcgat ctgcgatgcc cgcctgcaag tggcgacggc
      721 cgcggcgatc gaccgctatc tccagcgcgt cggggtcgcc gagtcgctcg ccgcggtgtt
      781 cgaggactga cgcgtcgatc agggctgcag ctgcggtggt ggacggagcc atcgattggc
      841 acgcgtcgtg cctggcggcc ccgctgcggg tggtaggcgg ggtcgcgctg ccggccggac
      901 cgcggtaacc tattgacgac gagcagacgg cgcttcatcg tggtcgctga tgccgacgta
      961 cgagtatcgc tgcagcgcct gtcaccgcga gttcgagtac cagcagaaga tgagcgacgc
     1021 ggacctcgtg cactgcgagg cctgcggcca ggacaagctg gagcggctga tctcctggac
     1081 ctcggtgcgg tcgacggggg gcattcggcc cgttcattat tccgcccagg ggatacaggg
     1141 attgcccccg gccgaccgca gccgggggcc gcagcggttc accagcgcgg agccggctgc
     1201 gccgtgtggc gatgcctgcg cctgcgcgcc ggcgacgggc ggcaccgact ccgagtagcc
     1261 gcggcctact ggcagaacgc ggtgcggaac gcggcggcct cgtcctgcga cgtcgtgccg
     1321 gcgcagcgct ggttggcctg cggatcgcgc gcgatcggcg agccgagcgt gcagcggacg
     1381 gtgttgtcgg ggaggcagcc gttgtcccag ctgcacatgc agtcggccgg gtcgatggtc
     1441 gcggtcaggc cgagcccgaa gccgatcgcg ccgatcgtcg tgttgatcgc gcgctggccg
     1501 gtgacgttgt cggcgatcca tgcgacgtcg tcggggcggg tgtcgccgca gtcgagctgg
     1561 ttgaccgccg cgccgaagtt cgagccgacc atgctcgcgg tgccgccgta cacgatcatc
     1621 acgtagttcc cggagctcgg ccggatccgg gtcacggcga tcgggaatcg cgccagctgc
     1681 cgggtgacgc cgtcgacgat ggtctggatc tgcaggtcgc ggccggtgtc ggtctggcgg
     1741 tacggcggcg ccgcgccgtt catcttgttc atccactgcg ccgtgttctg cgtggcgtcg
     1801 ctggggccct gggtcagcgt ctggccgtcg aagttgaggt agagcgtccg gcgcgtgcag
     1861 gtcgtgagcc ccgcgtcgcc ggtcgggccg gggccaccgc cggcgccggc gtcgggcgtg
     1921 gtgatgccgc cgaggctcgg atcgcggccg tccgagatct gcgcactgca gccgctgcac
     1981 agcgcaacgg cgagcgcgat ctgggccagc ttcatgacgc gactatgcga cttgtgacag
     2041 cgcgacgaca actgggagca cgtgccccac gcgggtgggc ctgtcctccc aggggggcgg
     2101 agccgccggg acgggttccg cgacgggcgc ggtcttacca cgtggacaac gcggcgcgcc
     2161 gcggggtgca gcgcggcgca acaacgagta cgttcgcccc gccatggccc gcatcaacga
     2221 gcactacgcc aagctgcccg cgagctacct gttccccgag atcggccggc gcgtccgcgc
     2281 ctaccaggcc gcgcaccccg acgccaaggt gatccggctc ggcatcggag acgtcaccga
     2341 gccgctcgcc ccggcgatcg tcgaggcgat gcacgccgcc gtcgacgaga tgggccggcg
     2401 cgagacgttc cgcggctacg gccccgagca gggctacgac ttcctgatcg acgcgatccg
     2461 cgagcacgac tacgcggcgc gcggggtgac gatcggcgcc gacgaggtgt tcgtcagcga
     2521 cggctccaag tgcgacagcg gcaacatcca ggagatcttc gcgctcgcgg cgcggatcgc
     2581 ggtcaccgac ccggtctacc cggtctacgt cgactccaac gtcatggccg gccgcaccgg
     2641 gcggatcggc gccgacggtc gctacgccgg gctgacctac ctggtcggca acgaggccaa
     2701 cgggttccag cccgagccgc cggccgagcc ggtcgacgtg gtctacctgt gctcgcccaa
     2761 caacccgacg ggcacggtcg cgacccgcgc ccagctcgag cgctgggtga gctgggcccg
     2821 ggcgaacgac gcgatcatcc tgttcgacgc ggcttacgag ggctacatcg ccgacccggc
     2881 gctgccgcac tcgatctacg aggtcgacgg cgccaaggac tgcgcgctgg agctgcgcag
     2941 cttctccaag cgcgccgggt tcacgggcgt gcgctgcgcg ttcctggtcg tgcccaagac
     3001 cgtcaccggg accgatcccg acggcaagcg cgtgtcgctg aacgcgctgt ggtcgcgccg
     3061 ccactcgacc aagttcaacg gcgcgtcgta cgtcgtgcag cgcggcgccc aggcggcgta
     3121 ctcgcccgcc ggcctggagc agacccgggc gcagatcgcg ttctacatgg agaccgcgcg
     3181 cctgttgcgc gaggggctgg tcgccgccgg gttcacggtg ttcggcgggg tccacgcgcc
     3241 gtacctgtgg ctgcggaccc gcgacggcgc gagctcgtgg gcgttcttcg accgcctgct
     3301 cgccgaggtc cagatcgtcg gcacgccggg cgccgggttc ggtccggccg gcgaggggta
     3361 cttccggctg tcggccttca actcgcgggc gaacatcgag gaggcgatcg cccggatccg
     3421 ccgcggcttc ggccgctagc gccatgcggt ggagcctcga agccgcgggg cacgccgagc
     3481 gcgcccccgc ccacgccatg atggtgcgcc ggtccggcag cggcccggag ctggtgtgga
     3541 tccacggcct cggcgagcag tcgcgcagcc tcgacgcgat cgctgcccac ccggcgctcg
     3601 ccgggttctc gcacgtgctg gtcgacctgc cgggctacgg ccgctcgccg tggccggagc
     3661 atcccgacga cctcgagcag ctcgccgacc ggctcgccgg ctggatcggc gaccgccgcc
     3721 ccgcgctgat cggccactcg atgggcggcg tgctcgcgac cctgatcgcc gagcggatcg
     3781 ccgtgcgcgc ggtgatcgac gtcgaaggca acctgtcgcg cggcgactgc acgcacagcg
     3841 cgctggccgc cgcgtacgcg ctcgacgact tcctcgccca cggctacgcc accatgcgcg
     3901 ccgaggtcta cgagggcggc cgcaccgacc tggcgctgcg cggctaccac gccgcgatga
     3961 ccatggcctc gccgcgcgtg ttccaccacc acgcgctcca gctggtcgcg atgagcgagc
     4021 ccgagaccct ggtcacccgg ctcgccgcgc tgcgcgcgcc ggtgcggttc atcgccggcg
     4081 gcctccccgg cgggatctgc gagcgcagcc gcgcgctgct cgatcgccac gcggtccgct
     4141 gggtcggcat cgagccggcg ggccactggg tccacatcga ccagcccgac cgcttcgcgg
     4201 ccgaggtcgc cgggttcctg gcgacgctgt gaacgtcgta gcatcactcg ggcacagcgc
     4261 cctcatctac catgtgtgcc agctcgagcc gatccgacac gctctccacg acgacctcga
     4321 ccgacgtagg gtcggcgacc cacgcccgca ccaccgcctg gaactggtac aggaagccgc
     4381 gcagggacac gaccccgccg gtcgcgcgcc gtgggtgcgc acgctcccag gcctcccgca
     4441 ggcgttggaa ctccaccctc gacatccgat cgcgcgatgt tagcgcagcg gctggtccga
     4501 aggaatctcc ggtctccgac ctgaagatcg agggtgttac agctccagca cccgaactgc
     4561 cttggtcgag ctggcgaatt ccaggatggc ggtgtctgcc gtgaccaggg aagcgccgag
     4621 ccgccgcgcg gtggcgacga tcatgcggtc ggaaggatcg gcgtgatccc atgcgaggtc
     4681 gacggcatcc acagcgattg ccggctcgag ggaagcgacg gtggccgaca gtgcgtcgag
     4741 cgcaccatgg atccattcga gcacatcgcc ggcgatcttg atcctcgctc gacgaactcc
     4801 gagcgcgatc tcccacgggg tgatcgccga gatgaacagc tcgttgtcgc gcccggctgc
     4861 ttcgatcttc ctggcgatct tcgccgggat gcggatgccc atgtgggccc agatcagcgc
     4921 gtgggtatca agcaccagca tcccatggct ccccggcccc cagctggaac agctcctcat
     4981 ccgtgcacag gatctcgatc gaaccccgcc ccttgcccag cccacgcggt gtgccgtgat
     5041 ccggtaccag ccgcgccatg accttgccgc gcttggtgat gacgacgggc tccccggttt
     5101 cggccacgcg atcgatcagc ttcaagcagg tggccttgaa cttgaccgcg ctgatcttca
     5161 taggaccagt atatgtggtc gtttcattcc gcgcaacgcg gagcaccgac ccgggtcgca
     5221 gtgggcgggc tacggcatgc gcttcggcgg gctgccgcgc ttgagcagct tggcgccctc
     5281 ggggtcggag gcgatgaagt cgagatcgtg gtcggttgcg cccttcgcga gcgacttctt
     5341 ggcgaccgga tcctcgtctt cgaccgccga caggtggtgg agcgcgccga gcgcggtctg
     5401 cttgtccttg cgcagcgagg cggcgcacgc gaggttgtag tacgcgagcg cgaacgagtc
     5461 gtcctcctgg gtcgcggcct ggaacttctg ctgggcggcg tcgaggttct tcgcggccag
     5521 gagcttcagg ccctcgacgt tcagcgcctg ggcgcgctgg cggtggctcg gcggcgcctg
     5581 ggcgttggcg gtcgagccgt cgagcgggat cacgacgagg tacctcggcg gaccgaagcc
     5641 gggacccgga tccttggccg ggacgtatcc ggcggcgatc gcgacggcgc ggccatcgcc
     5701 gcgccagaat gcctcgtacg cgtcggggac cggatccttg gggagccgga gctcgaagaa
     5761 gccgcgcgcc acccgcttgc cgccgcgcga gatcgcgacc tcgatgacgc catcgcgaac
     5821 ccggcggaga tcgtagtcct cgccgtgcgg gcccttcccg ttgtgccgcg gcgtcgcgac
     5881 cagctggcgg gccttgagca ccatgtgctg cgcatccagc gtgttgtcgg gaaacatcat
     5941 gtcccagcga tcggtgcagc cgtcgggatc gtccgggtcg ggcaccggga cgtccttggg
     6001 ccacgtcggc gacggcacgc cccggcgact gaggcacagc accgggacat ccatctcgtc
     6061 ggtgccggcg gtggtcatca cgaagaagct gccgtcctgc gaccagccga cgaagccgtc
     6121 gatgtagccg cgccgctggg cccgggcagc gccggcgtgc aggacgatcg cgagcgcgag
     6181 caagaaccca agatgcagcc ccggtcgata tcgcatcatg ctcccctccc gggagattct
     6241 actcgaaggc gggatccgct tccgacccgc gcgcggcggt cagtggtcgc tgccggatgg
     6301 ctccgaggat ggatcggg
//
LOCUS       DASVOR010001577         2031 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_191755,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001577 DASVOR010000000
VERSION     DASVOR010001577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191755"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..471)
                     /locus_tag="VF516_29700"
     CDS             complement(274..471)
                     /locus_tag="VF516_29700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111957.1"
                     /translation="MGKTRLLVEWVKRSDGVVADPSSPEVQRHYLTVALAARLRGLDG
                     VTFADGRSLLTGVADRAIESI"
     gene            820..>2031
                     /locus_tag="VF516_29705"
     CDS             820..>2031
                     /locus_tag="VF516_29705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111958.1"
                     /translation="MSAIFRAIDQRTGRSVALKLLQSHGSAEAIQRFARETQILAELH
                     HPHIVSYVAHGVDETTQPYLAMDWLEGENLAERMRRGPLSLRDTVAVALGVGAALAEA
                     HERGVIHRDLKPSNLFLPDRDPARVIVLDFGIARHMQHMGTMTRTGTVIGTPPYMSPE
                     QARGKREVGPPTDIFSLGSVLYECLDGRSPFFAEHMVATLVKVMLDEPRPIRTQRADV
                     PLAFASLIDRMLRKEPRERFPHGHALHEALRQLKLPAEESTDASTPIVSMTPPLIPQE
                     PRVQFPHDQAVHEALRRPELPAEESTQTASSTASVTPRLTPQEQRIVSLAIAVPRRDG
                     KERTTELVGDSDPPRCPPLPTQALAQLGARVGVLADGMVVATMLPAASASDQAFLAVR
                     CALLLEEHLPDV"
BASE COUNT          317 a          725 c          688 g          301 t
ORIGIN      
        1 cggttgagga tagcatcaac attccacgtg ccaccggttg agacggtcta acccggtccc
       61 ggcccggcgc ctgggcctgc tctgttgcga caccggtgcc agccggatgg ttgccgggcg
      121 ggggacctac acgtagcacc cgcgagccgg gagctgccgg ggcgggacgc ggcgcggccc
      181 cgggcgcgtc gcaggcgacg tcacggcaag ggcagcgggg ttgcggcccg gatcgccggg
      241 gcggaccagg ccgggccagt gcgtcgcggt cggtcagatc gactcgatcg cacggtcggc
      301 caccccggtg agcagcgagc gcccgtccgc gaacgtcacg ccatcgagac ctctgagcct
      361 ggccgcgagc gcaacggtca ggtagtggcg ctggacctcc ggcgacgatg gatccgcgac
      421 gacgccgtcg ctgcgcttga cccactcgac gagcagtcgc gtcttgccca cgcggcaccg
      481 ccccgggacc accgccagac cccttcgcgc cgcttcgata gcgcgagcaa gcgcgatagc
      541 tcgtcttgac ggtcgagcaa cttcctatgc aggaccgcat catgccatcc ggcataacaa
      601 gcggcaccac tgcccggccc cgttcatcct cgagcgttcg cgcgatcagg caacgtcggg
      661 gtgcatcgtg cgggttcgat cctcgcccgc gatcatgacg cagtcgtgtt catcgggact
      721 gccatggtgg tattcttgtc acggcgcatg agccatctcg tcgccggtga gctgttcgcg
      781 cagcgtttcc tggtagaggc gcagatcgct accagtggca tgagcgcgat cttccgcgcg
      841 atcgatcagc ggaccgggcg atcggttgcg ctcaagctcc tccagtcgca cggctccgcc
      901 gaggccatcc agcgcttcgc gcgcgagacc cagatcctgg ccgaattgca ccacccccac
      961 atcgtttcct acgtggcgca cggcgtcgac gagacaaccc agccctacct ggccatggac
     1021 tggctcgagg gcgagaacct ggcggagcgc atgcgccgcg gaccgctcag cctgcgcgac
     1081 accgtcgccg tggcgctcgg cgtgggggcg gcgctcgccg aggctcacga gcgcggcgtc
     1141 atccatcgcg acctcaagcc gtcaaatctc ttcctccccg atcgggatcc ggcccgagtc
     1201 atcgtgctgg acttcggaat cgcgcgccac atgcagcaca tggggacgat gacgcgcacc
     1261 ggcactgtga tcggcacgcc cccgtacatg tcgcccgagc aagcgcgtgg caagcgcgag
     1321 gttggaccac cgacggacat cttctccctc ggctccgtcc tgtacgagtg cctcgatggc
     1381 agatcgcctt tcttcgctga gcacatggta gcgaccctgg tcaaggtgat gctggatgag
     1441 ccgaggccga tccggacgca acgggctgac gtccccctgg cattcgcgag cctgatcgac
     1501 cggatgctgc gcaaggagcc ccgcgagcgg ttcccgcacg ggcacgcctt gcacgaagcg
     1561 cttcgccagc tgaagttgcc ggcggaggag tcgaccgacg cctcgacgcc gatcgtgagc
     1621 atgacgccgc cgctgatacc ccaggagccg cgcgtgcagt tcccgcacga ccaggccgtg
     1681 cacgaggcgc tccgccggcc agagctgccg gcggaggagt cgacccagac cgcgtcgtcg
     1741 acggcgagcg tgacgccgcg gctgacgccc caggagcagc ggatcgtcag cctggccatc
     1801 gccgtgccga ggcgcgatgg caaggagcgc accaccgagc tcgtgggcga cagcgacccg
     1861 ccgcggtgcc cgccgctccc cacgcaagcg ctggcccagc tgggcgcccg ggtcggggtg
     1921 ctcgccgatg ggatggtggt ggcgacgatg ctccccgccg cgagcgcctc ggatcaagca
     1981 ttcctggcag tgcgctgtgc gctgctcctc gaggagcatc tgccggacgt c
//
LOCUS       DASVOR010001578         1626 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_571993,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001578 DASVOR010000000
VERSION     DASVOR010001578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_571993"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1626
                     /gene="fusA"
                     /locus_tag="VF516_29710"
     CDS             <1..>1626
                     /gene="fusA"
                     /locus_tag="VF516_29710"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8111959.1"
                     /translation="VESGTRRLFHAAGEAGLARMVVVNRIDENLAGLPALVEQLRAAF
                     GPELHCINLPTGGGTDVLDCFDHDAGAADFGSVADVHREMLESTIEIDDHDLERYLAG
                     ERIDVAELRKDFVEAMNRGHVVPILFTSAIREVGVDDLLHILAAEAPSPVTGRPRRLR
                     RGDALAEIPCDPARPLLTHVFKVTTDPYLGKIAMIRVLQGQLDGTTPFVVAGGRKPLK
                     AGHLLKVEGREYVELTTAASAGDLVAIAKLDDLRIDAILRDPAAVDDLAAARPRYPEP
                     MYSLAVALHNKADEVKLSTAMARLVEEDPTLRATQDPQTHELVLSGLGELHLRITLEK
                     LKNRFGLDVTAHPPTIAYRETITARAEGHYRLKKQTGGAGQFGEVFLRVEPLPRGGGY
                     EFVDDVFGGAIPSQYIPACDQGCRDAMERGVVAGYPVQDVKVTVHDGKSHPVDSKDAA
                     FRTAAKFALRDAMQRAHPVLLEPIVALQISVPENHTGDVTGDLKNRRGQVVGVDVSNA
                     VTVVRALAPLSELGRYAGQLRSITGGLGSFVMEL"
BASE COUNT          252 a          591 c          566 g          217 t
ORIGIN      
        1 cgtcgagagc ggcacgcgcc ggctgttcca tgccgcagga gaggccggcc tggcgcgcat
       61 ggtggtcgtc aaccggatcg acgagaacct cgcggggttg cccgccctgg tcgagcagct
      121 gcgcgcggcg ttcggccccg agctgcactg catcaacctg ccgaccggcg gcggcaccga
      181 cgtgctggac tgcttcgacc acgacgccgg ggcggccgat ttcggctcgg tcgccgacgt
      241 ccaccgcgag atgctcgagt cgacgatcga gatcgacgac cacgacctcg agcgctacct
      301 ggcgggcgag cggatcgacg tcgccgagct acgcaaggac ttcgtcgaag cgatgaaccg
      361 cggccacgtg gtgccgatcc tgttcaccag cgcgatccgc gaggtcgggg tcgacgactt
      421 gctccacatc ctggccgcgg aggcgccgtc gccggtgacc ggccggccgc gccggctgcg
      481 ccgcggcgac gcgctggccg agatcccgtg cgatccggcg cgcccgctac tgacgcacgt
      541 gttcaaggtc acgaccgatc cgtacctggg caagatcgcg atgatccgcg tcctgcaagg
      601 ccagctcgac ggcacgaccc cgttcgtcgt ggccgggggg cgcaagccgc tcaaggccgg
      661 ccacctgctc aaggtcgagg gccgcgagta cgtcgagctg accaccgcgg catcggccgg
      721 cgacctggtc gcgatcgcca agctcgacga cctccggatc gatgcgatcc tgcgcgaccc
      781 ggcggcggtg gatgacctcg ccgcggcgcg cccgcgctac ccggagccga tgtactcgct
      841 cgccgtcgcg ctccacaaca aggccgatga ggtcaagctg tcgactgcga tggcgcgcct
      901 ggtcgaggag gatccgacgc tgcgcgccac gcaggacccg cagacccacg agctcgtgct
      961 cagcgggctc ggcgagctgc acctgcggat cacgctcgag aagctcaaga accggttcgg
     1021 gctcgacgtc acggcgcacc cgcccaccat cgcctaccgc gagaccatca ccgcgcgcgc
     1081 cgagggtcac taccggctca agaagcagac cggcggcgcg ggacagttcg gcgaggtgtt
     1141 ccttcgggtc gagccgctgc cgcgcggcgg cggctacgag ttcgtcgacg acgtgttcgg
     1201 cggcgcgatc ccgtcgcagt acatcccggc atgcgatcaa ggctgccggg acgcgatgga
     1261 gcgcggcgtg gtcgccggtt acccggtcca ggacgtcaag gtcaccgtcc acgacggcaa
     1321 gagccacccg gtcgacagca aggacgcggc gtttcgcacc gccgccaagt tcgcgctgcg
     1381 cgacgccatg cagcgcgcgc acccggtcct gctcgagccg atcgtcgcgc tccagatcag
     1441 cgtgccggag aaccacacgg gcgacgtcac cggcgacctc aagaaccgcc gcggccaggt
     1501 ggtcggtgtc gacgtgtcca acgcggtgac cgtggtgcgc gcgctcgcgc cgctcagcga
     1561 gctcggccgc tacgccggcc agctgcgatc gatcaccggc ggcctcggca gcttcgtcat
     1621 ggagct
//
LOCUS       DASVOR010001579         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_45658,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001579 DASVOR010000000
VERSION     DASVOR010001579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45658"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VF516_29715"
     CDS             <1..780
                     /locus_tag="VF516_29715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111960.1"
                     /translation="ALGGIAAGAVLMTRDPPPLREEREPNDDATHANRIAAGVPVTGF
                     LGKRHSPAEPDRDIYVVPWPSGSQRVVTVAVTGLPNIDITLGVTDGDGLHGATIDEND
                     IAAGEVLHRRRVDGPLVITVTGAIKDAVGKDPLPVENVSDPYTLTVTEEAPGAGETEP
                     NNGEADANPLVLTEEMRGYLDTRDDVDLLRWTGESGSYRIVVRSDGRSLVWRLPDGQA
                     RTPGATTVELHRGDVIRLERTDRGGKGAPGGDHRWSVVVTR"
     gene            833..>1596
                     /locus_tag="VF516_29720"
     CDS             833..>1596
                     /locus_tag="VF516_29720"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF004863.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEX8111961.1"
                     /translation="MIKRPKIAIVGAGGNVGAAVAQWAVQKELGDLVLIDIKAGPAEG
                     RALDLTQCGPWEGFNNTSFVATDKSEAMAGSDVVVMTAGVPRKPGQTREELININAGI
                     VKGICSDVKKHCPDAVLIVVSNPLDAMVFVAKQVTGFPRERVIGSAGVLDSARFRTYL
                     AMAAGVSVEDVQAIVLGAHTDKDMVPIVSHAMIGNVPVGKFLSPEKLNEVVESTKKGG
                     ATLTGLIGTSAWQAPGAGTALMVEAIVRNQGRVVPCT"
BASE COUNT          256 a          556 c          583 g          201 t
ORIGIN      
        1 gcgctcggcg ggatcgcggc cggcgcggtg ctgatgaccc gcgatccgcc gccgctgcgc
       61 gaggagcgcg agcccaacga cgacgccacg cacgccaacc ggatcgctgc cggcgtgccg
      121 gtcaccggct tcctcggcaa gcggcactcg ccggccgagc ccgatcgcga catctacgtc
      181 gtgccgtggc cgtcgggcag ccagcgggtc gtgaccgtcg cggtcaccgg gctgcccaac
      241 atcgacatca ccctgggcgt caccgacggc gacggcctgc acggcgcgac gatcgacgag
      301 aacgacatcg ccgccggcga ggtcctgcac cgccgccggg tcgacgggcc gctcgtgatc
      361 acggtcaccg gggcgatcaa agatgcggtc ggcaaggacc cgctgccggt cgagaacgtc
      421 agcgatccct acacgctgac cgtcaccgag gaggcgcccg gcgccggtga gaccgagccc
      481 aacaacggcg aggccgacgc caacccgctg gtgctgaccg aggagatgcg cggctacctc
      541 gacacccgcg acgacgtcga cctgctgcgc tggaccggcg agtccgggag ctaccgcatc
      601 gtggtgcgca gcgacggcag gtcactggtg tggcggctgc ccgacggcca ggcccggacg
      661 ccgggcgcca cgacggtcga gctccaccgc ggcgacgtga tccggctcga gcgcaccgac
      721 cgcggcggca agggtgcgcc cggcggcgac caccgctggt cggtcgtcgt cacgcggtga
      781 ggcgtcgggg tagttgcgcg ggcttcgcgg tcgggggtat gcctccggca ccatgatcaa
      841 gcgtcccaag atcgcaatcg ttggcgccgg tggcaacgtc ggcgcggccg tcgcccagtg
      901 ggcggtgcaa aaagagctcg gcgacctcgt gctcatcgac atcaaggccg gtccggccga
      961 gggtcgcgcg ctcgacctga cgcagtgcgg accgtgggag ggcttcaaca acacgagctt
     1021 cgtcgcgacc gacaagtccg aggcgatggc aggatccgac gtggtcgtga tgaccgccgg
     1081 cgtgccgcgc aagccgggcc agacccgcga ggagctgatc aacatcaacg ccggcatcgt
     1141 caaggggatc tgcagcgacg tgaagaagca ctgccccgac gccgtgctga tcgtcgtgtc
     1201 gaacccgctg gatgccatgg tgttcgtcgc caagcaggtc accgggttcc cgcgcgagcg
     1261 cgtgatcggc tcggccggcg tgctcgacag cgcgcggttc cgcacctacc tcgcgatggc
     1321 cgcgggggtc agcgtcgagg acgtccaggc gatcgtgctc ggcgcccaca ccgacaagga
     1381 catggtgccg atcgtctcgc acgcgatgat cggcaacgtg ccggtcggca agttcctgtc
     1441 gcccgagaag ctgaacgagg tcgtcgagag caccaagaag ggcggcgcga cgctgaccgg
     1501 gctgatcggc acgtcggcgt ggcaggcgcc gggcgccggc accgcgctga tggtcgaggc
     1561 gatcgtgcgc aaccagggcc gcgtcgtgcc gtgcac
//
LOCUS       DASVOR010001580         3389 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_366839,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001580 DASVOR010000000
VERSION     DASVOR010001580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366839"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF516_29725"
     CDS             complement(<1..345)
                     /locus_tag="VF516_29725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111962.1"
                     /translation="MLEALGMKTLAVLSLGGMFWAAAGCTDSTGDVDDASDVDAKAPV
                     AEVLGNQLGQAASQDGASTPASQDGQDASQASSSDDEVSTFDGDAASTFEDAAARHCD
                     SHNRLANNVPVHD"
     gene            complement(510..2693)
                     /locus_tag="VF516_29730"
     CDS             complement(510..2693)
                     /locus_tag="VF516_29730"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8111963.1"
                     /translation="MSEPLSLVLEFARNDTPEDPYGFRFKPQDYTLRTAHGGRKRVHL
                     AWSSEFLDDLDALHASSCDPAVVQRVGRALCSFLEPSGWAWHAQAIAAAARRSSPVLL
                     TIRSAAAELYALPWELLPLETTGQCVGELPGVLVRYEWPETHTVALQRARDPSRRRLL
                     VAWSAAAGEVPAAEHIDAIGAACRAAGWDFKPGRDVIQHASVGKLADALDQAKRDGAP
                     VVALHLLCHAGRAGRTWGLMLNGDEADEEGVAVDSWRLRQLLAPHADTLRLVVLAACG
                     SAHGREFDSVAQALHRAGVQAVVASRFPLSVQGAIRTAQTLYDAVLAQQATLEDAFLL
                     SRKQLARDASRLDWAGLQLYARAADGHQTRPFRAPAPDAAEVDGAPSGGARVDLDRLD
                     LPALLELQDQVRSALASRFESQQALLCVALADVDFRASLPDSAAHRRCHGLLDEVSAP
                     HSGRIFAVQGDSLRACYPSSKAALRAALSFIDAVTDHNYRCPREDQIVVSIGLHWGTV
                     LTNGKLVTGPAAPAVARIAELAGSSQLLLSQHALHRIPKVTQALCTPVAGAVGDELEL
                     YALPWGDERGLARQVRIEESGEQLALPSQDIIAFGRLDALADGTRANDIVLTHPDREA
                     QLAISRWHFELRRTRRGHILRAVSSQRTEVDGRTVESGEEAQVRPGTTVRLAQVMTLR
                     FLGSEQSYSPRDAVTLQHGMEGGMRRVTGKDGDEVSTAPPPPPRV"
     gene            complement(2712..>3389)
                     /locus_tag="VF516_29735"
     CDS             complement(2712..>3389)
                     /locus_tag="VF516_29735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111964.1"
                     /translation="AGHVGSETGHVLDTLAHDRPFVAALPRAVLADPDGPLAFVGHID
                     LAWAYSFFDLDDRPRRRPGRIAGVLQALLNGHRAGVAVRKLLQWFAEVNTELTALDDA
                     RARTGGASLDDREQIRRGHLWMLRQDLAGYALLGDPAIRLPIGGSGDGALRAVERGTD
                     GACAAVVARAEAAVLAVLRGDPVADVARCHGLAHAELARLVASFCAAGRAALEAAVPD
                     RAPDQGP"
BASE COUNT          473 a         1258 c         1193 g          465 t
ORIGIN      
        1 gtcgtgcacc ggcacgttgt tggccagccg gttgtggctg tcgcagtgcc gcgccgccgc
       61 gtcctcgaac gtgctcgccg cgtcgccgtc gaacgtgctg acctcgtcgt cgctgctgct
      121 cgcctggctg gcgtcctggc catcctggct cgccggggtc gacgcgccgt cctggctggc
      181 ggcctggccg agctggttgc ccaacacctc ggcgaccggc gccttggcgt ccacatcgct
      241 cgcgtcgtcc acatcgccgg tgctgtccgt acagccagcc gcggcccaga acatcccacc
      301 cagcgagaga actgcgagcg tcttcatgcc cagcgcttcg agcatgagtc atgccgatat
      361 ttctgatatg caactccgcg agatactgcg atgttgttcg atccgccgcg gtcactgacg
      421 cagtccgggg tgcacctccg aaggtccagg gcagcgcgcc catgctcgtg gccggtccgc
      481 accggctggc cgcaccggta tggccgagct cacacacgcg gcggtggtgg cggagccgtg
      541 gagacctcgt cgccgtcctt gccggtcacc cggcgcattc cgccttccat tccatgctgc
      601 aatgtaaccg cgtctcgggg ggagtacgac tgctcacttc ccagaaaccg taatgtcatc
      661 acttgtgcga gccgaacggt ggtcccgggt cgtacctgcg cctcctcccc ggactcgacc
      721 gtcctgccat cgacctcggt gcgttgactc gacaccgcgc gcaggatatg gccccgccgg
      781 gttcgccgga gctcgaagtg ccagcggctg atcgccagct gcgcctcgcg atcgggatgg
      841 gtcagcacga tgtcgttggc ccgggtgccg tcggccagcg cgtcgagccg gccgaacgcg
      901 atgatgtcct ggctgggcag cgcgagctgc tctccggact cctcgatccg gacctggcga
      961 gcgagcccgc gctcgtcgcc ccacggcaac gcgtacagct cgagctcatc gccgaccgcc
     1021 ccggcgaccg gcgtgcacag cgcctgcgtc accttcggga tgcgatgcag cgcgtgctgc
     1081 gacagcagga gctggctcga tccggcgagc tcggcgatcc gcgccaccgc gggcgccgcc
     1141 gggccggtga ccagcttgcc gttggtgagc accgtgcccc agtgcagccc gatcgacacg
     1201 acgatctggt cctcgcgcgg acagcggtag ttgtgatcgg tcaccgcgtc gatgaacgac
     1261 agcgccgcgc gcagcgccgc cttgctcgac ggatagcacg cgcgcaggct gtcgccctgg
     1321 accgcgaaga tccggccgct gtgcggcgcg gagacctcgt cgagcaggcc gtggcagcgc
     1381 cggtgcgccg cggagtcggg caggctcgcg cggaagtcga cgtccgccag cgccacgcac
     1441 agcagggcct gctgcgattc gaaccgcgac gccagcgcgc tgcgcacctg gtcctgcagc
     1501 tcgagcagcg ccggcaggtc gagccggtcg aggtcgacgc gagctccgcc cgacggcgcg
     1561 ccatcgacct ccgccgcgtc gggtgcgggc gcgcggaatg gacgggtctg gtgtccgtcg
     1621 gccgcgcggg catagagctg gagcccggcc cagtcgagcc ggctcgcgtc gcgcgccagc
     1681 tgcttgcgcg acagcaggaa cgcgtcctcg agcgtcgcct gctgggcgag gacggcgtcg
     1741 tacagcgtct gcgcggtccg gatcgcgccc tgcaccgaca gcgggaaccg cgacgcgacc
     1801 accgcctgga cccccgcgcg atgcagcgcc tgggcgacgc tgtcgaactc gcggccgtgg
     1861 gcgctgccac acgcggcgag cacgaccagg cgcagcgtgt cggcgtgcgg cgccagcagc
     1921 tggcgcaggc gccacgagtc gacggccacg ccttcctcgt ccgcctcgtc gccgttgagc
     1981 atcaggcccc aggtccggcc cgcgcgcccg gcgtgacaca agaggtgcag cgccaccacc
     2041 ggcgcgccgt cgcgcttggc ctgatcgagc gcgtcggcga gcttgccgac cgaggcgtgc
     2101 tggatcacgt cgcgtcccgg cttgaagtcc cagcccgcgg cccggcacgc cgcgccgatc
     2161 gcgtcgatgt gctcggcggc cggcacctct ccggcggccg cggaccacgc gacgagcagc
     2221 cggcgccggc tgggatcgcg cgcgcgctgg agcgccacgg tgtgagtctc gggccactcg
     2281 tagcggacca gcacgccggg cagctcgccg acgcactgtc cggtcgtctc cagcggcagg
     2341 agctcccacg gcagcgcgta gagctcggcc gccgccgatc ggatcgtcag cagcaccggc
     2401 gacgaccggc gcgcggccgc ggcgatcgcc tgtgcatgcc acgcccatcc ggacggctcc
     2461 aggaagctgc acagcgcgcg cccgacgcgc tggacgaccg ccggatcgca ggacgacgcg
     2521 tggagcgcat ccaggtcgtc gaggaactcg ctcgaccacg ccaggtgcac gcgcttgcgg
     2581 cccccgtgtg cggtccgcag cgtgtagtcc tgcggcttga accggaaccc gtaagggtct
     2641 tctggcgtat cgttgcgagc aaactcgagc acgagcgaca ggggttcgga cacgcactgc
     2701 gcatgtgcaa gtcatggacc ctggtccggc gcacggtccg gaacggcggc ttcgagcgca
     2761 gcgcgacctg ccgcgcaaaa gctcgcgacg aggcgcgcga gctcggcgtg agcgaggcca
     2821 tgacatcggg ccacgtccgc caccggatcg ccccggagga ccgcgagcac ggcggcctcc
     2881 gcgcgcgcca ccaccgcggc acacgcaccg tcggtgccac gctcgaccgc gcgcagcgcg
     2941 ccatcgccgc tcccgccgat cggcaaccgg atcgccggat cgccgagcag cgcatagccc
     3001 gcgaggtcct ggcgcagcat ccatagatgg ccgcggcgga tctgctcgcg atcgtcgagc
     3061 gatgccccgc ccgtgcgcgc ccgggcgtcg tcgagcgcgg tgagctcggt gttgacctcg
     3121 gcgaaccact gcaggagctt gcgcaccgcg acaccggcgc ggtgtccgtt cagcagcgcc
     3181 tggagcacgc cggcgatccg gccgggccgc cgccggggac ggtcgtcgag atcgaagaag
     3241 ctgtaggccc acgccaggtc gatgtgaccg acgaacgcga gcgggccgtc cggatcggcg
     3301 agcaccgccc tgggcagcgc ggccacgaac ggacggtcgt gcgcgagcgt gtccaggacg
     3361 tgtccagtct cgctcccgac atgtccagc
//
LOCUS       DASVOR010001581         4895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_74456,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001581 DASVOR010000000
VERSION     DASVOR010001581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4895
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74456"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            886..1248
                     /locus_tag="VF516_29740"
     CDS             886..1248
                     /locus_tag="VF516_29740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111965.1"
                     /translation="MSSGRGFDARGEIVQEYSARIIAWEGDNYDGGSTVGNAEFYIVK
                     VAAAMNGGISLMDAIDSVNQDVYEYGAAVLDLDTGDIRADLAERFEYISGDLMFLHVM
                     KILPLTAGETSDSSRQAA"
     gene            complement(2412..3092)
                     /locus_tag="VF516_29745"
     CDS             complement(2412..3092)
                     /locus_tag="VF516_29745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111966.1"
                     /translation="MRNDESWRALHHQLCGISKRRAALDAEEARCLREAIAVQLWRRL
                     GYVHLNEYLERELGYAPQAGIERVRVAQALALLPRIEASLGEGVLSFSAVRELTRVAQ
                     PDTEQAWLDAAYGKNLRQIEAMVSGKKPGDRPEDPARPELMKRILRLELSPSAFAAFR
                     QVQAVLADEHGGRLDDSALIETLCRRALEGGAEVGRPAHQIAFRCANRAAAAGRTEPG
                     ARSRSAPR"
     gene            complement(3233..3781)
                     /locus_tag="VF516_29750"
     CDS             complement(3233..3781)
                     /locus_tag="VF516_29750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111967.1"
                     /translation="MRGARAKLRLAPHPAARSLAHVRAVSPVMVVVLLVGIAAAVALA
                     QPAPSALTAEIHLSADLYELDNTQAGADHVRTVWHVRDADDGDFGVDLLRRHYAEAHA
                     AANTPATRKWRTELGRPIEPFRIVGNIYYVGATDIASYLISTAQGLIPLDTGTREMLP
                     RALRTVAHPTGLEPSPRGPARP"
     gene            complement(3832..4758)
                     /locus_tag="VF516_29755"
     CDS             complement(3832..4758)
                     /locus_tag="VF516_29755"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX8111968.1"
                     /translation="MRVLVAWGSRHGGTEGIARRIADAMREASVDVELRAAREVDTLD
                     GYDAAILGGALYANRWHRDARRLITRHLAALRRIPVWLFSSGPLDESAARAPIAPVTE
                     VAILMERIGAVGHATFGGRLEPDVTGWMAHAMAPSHSGDWRDPAQIRAWAIDIAARLS
                     TARPRVPITPPARSLPRLVAHAVAGWSACAVVMAGLLAVASTAVAVAVHAIAAPLIFA
                     VIARSYFGARGAREPLPVAVAFTAIVAGLDAAIVAGLIQHRFAMFASVGGTWLPLALI
                     FAATWATGAIVATLPWPKPTPPAPTRPASAHP"
BASE COUNT          733 a         1709 c         1731 g          722 t
ORIGIN      
        1 cggcctgctc gacagcccgg gcctcctgca tcgcctgctg atcggggacc gcgaggcaga
       61 ggatggcgcc ggcgccgacg gggccgagga tcacggccgc acgacgtgag gcgcgaaccg
      121 cgcggtgttg tcggtgacgt agctcgcggt ctcgcggacc cgatcccggc ggcgcactcg
      181 ccgatcagcc acgagccgat caccggccgc atcccatcgt ggtcgccgag ctcggcgtag
      241 gtctggtaga cgaacccggt gtcggcgtag tattccagga gcctgggcac gccgtcccag
      301 tagacgccgt ggtcgggcgt ggtgtggaac accaggccct gggcttcgac gcgggcttgc
      361 cagtcggggc cgtgggtcga tccggcggcg ccgcatcagc cgccggacgc ccagaagtag
      421 tggccgaagc cgccgcggaa cacgtggtgg tgacggtgct ggccggcggc gggttaagtc
      481 ggtgtgaacg gtgcgactgc cgacgatccg cggcggcggg ttaagtcgaa gtgaacgacg
      541 taaccgccgc ggatcggctg ctacagtggc gtgccgcagc tgccgcacag gcccgaaggc
      601 aggtgtgacg ttgcaggcgc gatcgtctgc attcgccgcc gatcgtggaa cgccgtcagc
      661 aggaagctcc gcgggcgtca cctctgcccc cacgtggggg cggcgatgcg atgtcttcgc
      721 gcgcggacga acccgtgccg aggtcaccag cgagggctcc gcggcagagc gtatcgggtc
      781 gccgtcgagc gcgtcgagcg cgaatcaccg ctgcggcatc gccgctccga atcggaggtg
      841 ttaccgtctt ctgcatgggt taccgtctag aggttgaggg aagtcatgtc ctcgggccga
      901 ggattcgatg cacgagggga gatcgtccag gagtattcgg ccaggatcat cgcgtgggag
      961 ggtgacaact atgacggggg atctaccgtc ggcaacgcgg agttctacat cgtcaaggtc
     1021 gctgctgcga tgaacggcgg catctcgctg atggacgcga tcgattccgt caatcaggac
     1081 gtctacgagt acggtgctgc ggtgctcgat ctagataccg gcgacatacg cgccgatctc
     1141 gccgagcggt tcgagtacat cagcggcgac ctcatgttcc tgcacgtcat gaagatcctg
     1201 ccgcttaccg cgggagagac atcggactcg tcgcggcaag ccgcctgatt gagagctacg
     1261 cgagcggcct cgtcgtctgc cgctcgcatc cgctgcaata cgttggcgac gagccggaga
     1321 aatccgcccc ggacggcatg gagtacgagc gattctcgaa agaccgacgc caggccaagc
     1381 gaaactccag aagtactggt cttgcttggg attcgagcct gtcgacgacg atggcatcta
     1441 cgccctggga acggcgagcg cactgccgcc catgaaggca cgcaccacgc cgaaacgggc
     1501 gcaacaccgt ggtggctcac cgatgccaac gaggaaacca cggggagccc ggcgtcaccg
     1561 cagcgcctcg cgccgcggaa cctgacccgc cgatatcccc cagttcccag agcgcctcgc
     1621 cgcggctcat catcctgcgg gagcctgcga cgctgacgat gccggtaccg tgcagcgccg
     1681 gagtgcctcg cggaggagtt gctccagggt cgcctcggcc cccacgtggg ggcgagcctg
     1741 ggccaccgca gcacgcgcct cgcgcagctt gaaccccgct gtgatcagcg ccgtgcaggc
     1801 gtcactgtca acggtcgaga tctccatctc gtcatgaacg atgtcgggtc catcgaatgg
     1861 tgtgcaggcc ggcgccagcc gttgcacagg tgtcggagcg tcgccgtcac gagcggtgtc
     1921 ctcggcccgt gggtgcgctg gggacagacc gctccggccg acgctggagc tgccgtcccg
     1981 cgccggtgtc gcacgccgcg tgcgctcggg gccctcgctc cggacagcca cgccctctga
     2041 tgaccgaggc acgacgggtg cattgtcact ccttacctcc gcggtcgccc gctccgctgc
     2101 ctcgcgccac cagaactgca gcgcatgagg cgcctcgccg cggacgatca gctggcccgc
     2161 gtggagcagc gcgtggtggc ccgagcacaa ggtcagaagg ttcgacagct cgttcccgcc
     2221 accgtcgctg cggaactgga tgtgatggac atcgaggttg cgcgcggacc ggcagccagg
     2281 gacggtgcag ccgtggtggt cgcgtgcgag cacctggcgg cgaagccgcg gcgtgagggt
     2341 ggacgtcaca cgcgcaggct cgtccgcctc gagcgaaccg atcagctcgg cgtcgcaccg
     2401 cgcgcggtcg atcacctcgg cgctgacctc gatctcgcgc ccggctccgt tctgccagcc
     2461 gcggcggcac gattcgcaca cctgaacgcg atctggtgtg ccggacgccc gacctccgct
     2521 ccgccttcga gcgcgcgccg acacagcgtc tcgatcagcg cgctgtcatc gaggcggccg
     2581 ccgtgctcgt ccgccaggac cgcctggacc tggcgaaatg ccgcgaaggc cgatgggctg
     2641 agctccaggc gcaggatgcg cttcatcagc tcgggtcgcg ccggatcctc gggccgatcg
     2701 ccgggcttct tgcccgacac catggcttcg atctgccgca ggttcttgcc atatgcggcg
     2761 tcgagccagg cctgctcggt gtccggctgt gccacgcggg tcagctcgcg caccgccgag
     2821 aatgacagca caccctcgcc gaggctcgcc tcgatccgag gaagcagcgc gagcgcctgg
     2881 gcgacgcgca cccgctcgat ccctgcctgc ggggcgtacc cgagctcgcg ctccagatac
     2941 tcgttcagat gaacgtagcc gagccgccgc cacagctgga ccgcgatggc ctcgcgcaga
     3001 caccgcgcct cctcggcatc gagcgccgcg cgccgcttcg agattccgca cagctgatgg
     3061 tgcagcgcac gccatgattc gtcattcctc accgaggcca tcgtgattct cctggctgat
     3121 ctgcacgcgc cctcccttcg cgtgcgcgcc atggatacac cccccgtatg acatcattct
     3181 gtattcgacc tcgttgcggt gtcgtccgcg tccgatcgag tgattcctct cgtcacggtc
     3241 gagctgggcc acgcggagat ggttcgagcc ccgtaggatg cgcgacggtg cggagcgctc
     3301 gtggcagcat ctcgcgcgtg ccggtgtcga gggggatcag gccttgtgcg gtcgagatca
     3361 ggtacgacgc gatgtcggtt gcgccgacgt agtaaatgtt gccgacgatg cggaacggct
     3421 cgatcggccg gcccagctcg gtgcgccatt tccgggtcgc cggggtgttc gcggcggcgt
     3481 gggcctcggc gtagtgccgg cggagcagat cgacgccgaa gtcgccgtcg tccgcgtcgc
     3541 gcacgtgcca gacggtgcgg acgtggtccg cgccggcctg ggtgttgtcg agctcgtaga
     3601 ggtccgccga caggtggatc tcggcggtca gcgcgctcgg ggcgggctgc gccagcgcga
     3661 ccgcggccgc gatcccgacg agcagtacga cgaccatgac cggggaaacc gctcgaacat
     3721 gcgcgaggct cctggccgcc gggtgcggcg cgagccgtag cttggcccgc gcgccgcgca
     3781 tccgttcatc gaagcttgat gacggcgcac cgaggggtga tgcccgtccg gctacgggtg
     3841 ggcggacgcc ggcctcgtcg gcgccggcgg ggtcggcttc ggccacggca gggtcgcgac
     3901 gatcgcaccg gtcgcccagg tcgctgcgaa gatcagcgcg agcggcagcc aggtcccgcc
     3961 gacgctcgcg aacatcgcga agcgatgctg gatcagcccc gccacgatcg cagcgtcgag
     4021 cccggcgacg atcgcggtga acgcgaccgc gaccggcagc ggctcgcgcg cgccgcgcgc
     4081 cccgaagtag ctccgtgcga tcaccgcgaa gatcagcggc gccgcgatcg catgcacggc
     4141 gaccgcgacc gcggtgctgg cgaccgcgag caggccagcc atgaccaccg cgcacgcgct
     4201 ccacccggcg acggcgtggg cgaccaggcg aggcagcgag cgcgcgggag gagtgatcgg
     4261 cacccggggc cgggcggtgg acagccgcgc cgcgatgtcg atcgcccacg cccggatctg
     4321 cgccggatcg cgccagtcgc cgctgtggct gggcgccatc gcgtgcgcca tccagccggt
     4381 gacgtcgggc tccagccgcc cgccgaaggt cgcgtggccg accgcgccga tccgctccat
     4441 caggatggcg acctcggtca ccggcgcgat cggcgcgcgg gcggccgact cgtcgagtgg
     4501 cccgctggag aacagccaca ccgggatccg tcgcagggcg gccaggtgtc gggtgatcag
     4561 ccgtcgcgcg tcgcgatgcc agcggttggc gtacagcgcg ccgccgagga tcgccgcgtc
     4621 gtacccgtcg agcgtgtcga cctcacgcgc ggcgcggagc tcgacgtcga cgctcgcctc
     4681 gcgcatcgcg tccgcgatcc tcctcgcgat gccttcggta ccgccgtgtc gagagcccca
     4741 ggccacgagc acgcgcatgg caaacctccg acccgagcgc ttgcaggaac cgggccccgc
     4801 agcgaccgcc accccggccg cgggctgcgg tgttgcgcgc gccgtacgcc cgcgatgcgc
     4861 gcccgctgcg tgctcgacgc gccgccgcgc acgga
//
LOCUS       DASVOR010001582         3262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_17134,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001582 DASVOR010000000
VERSION     DASVOR010001582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3262
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17134"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VF516_29760"
     CDS             complement(<1..873)
                     /locus_tag="VF516_29760"
                     /inference="COORDINATES: protein motif:HMM:NF016143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEX8111969.1"
                     /translation="MKWTPGTENDDVIDERGDSGGGGGGGGGGFGAMHLTGGGLATVV
                     VIVVVARLFGVDVSGLLGRNHGSGPVATRRPDQPHPPTGPDPDRELVDFVKFAMKDIQ
                     HVFETRFQAEGKPYRHAKLVLFTSAIDTGCGRSSSAIGPFYCPADEHAYIDLSFYREL
                     RQRFGAPGNFAQAYVLAHEMGHHLQKLLGVEQRAQGMGRGHGRNDISVRTELQADCFA
                     GVWGHDAKGESLLEAGDIEGAITAATAIGDDRLQKQAGREVNPETFTHGTSAQRVRWF
                     RTGFEAGRFDACNTF"
     gene            899..1918
                     /locus_tag="VF516_29765"
     CDS             899..1918
                     /locus_tag="VF516_29765"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX8111970.1"
                     /translation="MSPRAKSTAKDAKPATPRKRKKKAEAGSVGLAAGEVAAGSPPGT
                     LAPLRARIEATGGAVLTTYREPFGGNWVTLAALPLDKIKPTPYQRELSKAHTDRLAAV
                     IPKVGRFLDPVIAVADPDGDGFITPNGMHRLAALAALGAKTVIALVIPEPEIAFRILA
                     LNTEKAHNLKDKALEVIRMARAIAADPKKAGTSESDVAFELEQPQLVTLGVCYENNAR
                     FSGGAYNSVVARTEVFSTEPMKKSLVLRETHAERLLELDAAVTAAVARLKESGFTSGY
                     LKPIVVARINPLRFIKAGKDDARPDFDKTIDRMLDGAKKFDPSKVRAQDVAIAAAVGG
                     GEESI"
     gene            complement(1933..2436)
                     /locus_tag="VF516_29770"
     CDS             complement(1933..2436)
                     /locus_tag="VF516_29770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111971.1"
                     /translation="MKQTLLGSMIVFASFAFVGTARAGGQPNSIGVGAELQVSGVGGL
                     SVNYDAGKFHAGGFFGYFDPQGPNNSTVDLGGRFFFHIASTAASDFSLGGSIGIENAN
                     DPGTNTRHTDVFIEPSFQIRAFIVPNVALSFTGGIVIGAADISELDLTSNTSGRVTGM
                     AGIHYYF"
     gene            2496..>3262
                     /locus_tag="VF516_29775"
     CDS             2496..>3262
                     /locus_tag="VF516_29775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111972.1"
                     /translation="MLSAELDLCTIDARHWKNWWRLLVPPRVLAPARWALAILDRGTL
                     VQLIVRGEGAHEIVPLPGLPEISAKALASYARTLGVAAVIVVERRLIAELSAEIEAAL
                     ELDQDFVAQGLIVLRALKRHAGRGVWTEPALLELLPTPSYEAVQRTFDLLVPDRSAMV
                     ACVIDDDRRAVHASIIAVKAGGDVTRAATHRAIADLVPEASLARDWETGYRRVLTAVE
                     DRFARPSIALFAERATVLGVLTGPSDQLARELNARRVV"
BASE COUNT          500 a         1156 c         1118 g          488 t
ORIGIN      
        1 gaacgtgttg cacgcgtcga accgcccggc ctcgaacccg gtgcggaacc atcgcacgcg
       61 ctgcgccgag gtgccatggg tgaacgtctc ggggttgacc tcgcggccgg cctgcttctg
      121 cagccggtcg tcgccgatcg cggtcgccgc ggtgatcgcg ccctcgatat cgccggcctc
      181 gagcagactc tcgcccttgg cgtcgtggcc ccacacgccc gcgaagcagt cggcctggag
      241 ctcggtgcgc accgagatgt cgttgcggcc gtggccgcgg cccatcccct gcgcgcgctg
      301 ctcgacaccg agcagcttct gcaggtggtg acccatctcg tgggcgagca cgtacgcctg
      361 tgcgaagttc cccggcgcgc cgaaccgctg ccggagctcg cggtagaacg acaggtcgat
      421 gtaggcgtgc tcgtcggccg ggcagtagaa cgggccgatc gcgctcgacg accggccgca
      481 cccggtgtcg atcgcgctgg tgaacagcac cagcttggcg tggcggtacg gcttgccctc
      541 ggcctggaac cgggtctcga acacgtgctg gatgtccttc atcgcaaact tgacaaagtc
      601 gaccagctcg cggtcagggt ccgggccggt cggcggatgc ggctggtccg gacgccgggt
      661 cgcgaccgga cccgagccgt gattgcggcc gagcaggccg ctgacgtcga cgccgaacag
      721 ccgcgcgacc accacgatga cgaccacggt ggccagtccg ccgccggtca ggtgcatcgc
      781 gccgaagcct ccgccgccgc cgccgccacc gccgccgcta tctccccgct cgtcgatgac
      841 atcgtcgttc tcggtcccag gagtccactt cacaccagca gggtactaca gtgacgccat
      901 gtcgcctcgt gccaagtcca ccgccaagga cgccaagccg gccacgccgc gcaagcggaa
      961 gaagaaggca gaggccggtt cggtgggcct ggcggccggc gaagtcgccg ccgggtcgcc
     1021 gccggggacg ctcgccccgc tgcgcgcccg gatcgaggcc accggcggcg ccgtgctcac
     1081 cacctaccgc gagccgttcg gcggcaactg ggtgacgctc gcggcgctgc cgctcgacaa
     1141 gatcaagccg accccttacc agcgcgagct gtccaaggcc cacaccgatc ggctcgccgc
     1201 ggtgatcccc aaggtcggcc gcttcctcga cccggtgatc gccgtggcgg accccgacgg
     1261 cgacggcttc atcacgccca acggcatgca ccggctcgcc gcgctcgccg cgctcggcgc
     1321 caagaccgtc atcgcgctgg tcatccccga gccggagatc gcgttccgca tcctcgcgct
     1381 caacaccgag aaggcgcaca acctcaagga caaggcgctc gaggtgatcc gcatggcgcg
     1441 cgcgatcgcg gccgatccca agaaggccgg cacgagtgag tccgacgtcg cgttcgagct
     1501 cgagcagccc cagctggtca cgctcggcgt gtgctacgag aacaacgcgc ggttctcggg
     1561 cggcgcctac aactcggtgg tcgcgcgcac cgaggtgttc tccaccgagc cgatgaagaa
     1621 gagcctggtc ctgcgcgaga cccacgccga gcggctgctc gagctcgacg cggcggtgac
     1681 cgccgcggtg gcgcggctca aggagtccgg gttcaccagc ggatacctga agccgatcgt
     1741 cgtcgcccgg atcaacccct tgcggttcat caaggccggc aaggacgacg cgcggccgga
     1801 cttcgacaag acgatcgacc gcatgctcga cggcgcgaag aagttcgacc cgtcgaaggt
     1861 ccgggcccag gacgtcgcga tcgccgccgc ggtcggcggt ggtgaggaat cgatctgatc
     1921 gactagcgcg gctcagaagt agtagtggat gccggccatg ccggtcacgc gccccgacgt
     1981 gttgctcgtc aaatcgagct ccgagatgtc ggccgcaccg atcacgatcc cgccggtgaa
     2041 cgacagcgcg acgttcggca cgatgaacgc gcggatctgg aagctcggct cgatgaacac
     2101 gtcggtgtgg cgggtgttgg tgccggggtc attggcgttc tcgatgccga tgcttccgcc
     2161 gaggctgaag tccgacgccg cggtcgatgc gatgtgaaag aagaaccggc cgccgaggtc
     2221 gaccgtcgag ttgttgggcc cctgcggatc gaagtagccg aagaacccgc ccgcgtggaa
     2281 cttgccggcg tcgtagttga ccgacaggcc gcccacgccg ctgacctgga gctcggcacc
     2341 gacgccgatc gagttaggct ggccgcccgc gcgcgcggtt ccgacgaacg cgaaggatgc
     2401 gaacacgatc atcgatccaa gtagggtctg cttcatgcgc ggcatgctta cccgtcgcgt
     2461 tgcccggcgc aagttcgtca atggtatggt gaagtgtgct ttccgccgag ctggatctgt
     2521 gtacgatcga tgcacgccac tggaagaact ggtggcgctt gctcgttcca ccgcgcgtgc
     2581 ttgcgccggc gcgctgggcc ctcgccatcc tcgatcgcgg aacgctggtg caactcattg
     2641 ttcgaggtga gggtgcgcac gagatcgtgc cgctgcccgg gctccccgag atctcggcga
     2701 aggcgctcgc gagctacgcc cgcacgctcg gcgtcgccgc ggtgatcgtc gtcgagcgac
     2761 ggctgatcgc ggagctgtcc gccgagatcg aggccgcgct cgagctcgac caggacttcg
     2821 tcgcccaggg tctgatcgtg ctgcgcgcgc tcaagcgcca cgccggccgc ggcgtgtgga
     2881 ccgagcccgc gctgctcgag ctgttgccga cgccgagcta cgaggcggtg cagcggacgt
     2941 tcgacctgct ggtgccggat cgctcggcga tggtcgcgtg cgtgatcgac gacgatcgcc
     3001 gcgcggtcca cgcctcgatc atcgccgtca aggccggcgg tgacgtgacc cgcgccgcta
     3061 cccaccgcgc gatcgccgac ctggtccccg aggccagcct cgcgcgcgac tgggagaccg
     3121 gctatcgccg cgtgctcacc gccgtcgagg accgcttcgc ccggccgagc atcgcgctgt
     3181 tcgccgagcg cgcgacggtg ctcggcgtgc tgaccgggcc gagcgatcag ctcgcgcgcg
     3241 agctcaacgc ccggcgcgtc gt
//
LOCUS       DASVOR010001583         1997 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395396,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001583 DASVOR010000000
VERSION     DASVOR010001583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1997
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395396"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="VF516_29780"
     CDS             <1..837
                     /locus_tag="VF516_29780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEX8111973.1"
                     /translation="RAEDVLEAKRLLTVDQVSIPVIAKIEKPQALERLGEIIDAADGT
                     MVARGDLGVELGPEKVPLWQKRIIEETNRRGKIVITATQMLESMITQPRPTRAEASDV
                     ANAVLDGTDALMLSAESASGLFPVEAVRTMARIIDEIERSAYYRANVEVPDIQLAVPT
                     NAIAHAAITAARAMRLKTIVVVSESGGAARLVSEYRPDANIVCLTTNEVSFRRLALFW
                     GVTPVLISPSATTEELIDRVEATLIERNLALPGENVLITMAVPVGSGMQTNVLKIHEI
                     QR"
     gene            973..>1997
                     /locus_tag="VF516_29785"
     CDS             973..>1997
                     /locus_tag="VF516_29785"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8111974.1"
                     /translation="MATPRSIDAGVGIGEIFVDKYRVDRILGHGGMGVVAMCTHLALN
                     EHVAIKMLRKDVLDDADAVERFMREAQAAVKLKSEYVARVIDVGRSSANVPYMVMEYL
                     DGEDLGQLLEDRRTLGVPWAVEITMQCCEALAEAHSLGIVHRDVKPTNLFVTWRPDGS
                     AIIKVLDFGISKALTGNSMALTQTQSLLGTPAYMSPEQMRSARMVDARTDIWSLGTVL
                     YEMLEGRRPFEAESFSEMCVKVAVDDPGAMTQTPPGLQLVVLRCLEKSPDQRYPSMAE
                     LARDLVPFAQDPHQAQVLVERMTRTLRRSQAQPVEWSPASSSGSIRLPSGERVAETSP
                     APAAAPAP"
BASE COUNT          320 a          699 c          696 g          282 t
ORIGIN      
        1 cgcgccgagg acgtgctcga ggccaagcgg ctgctcaccg tcgaccaggt ctcgatcccg
       61 gtgatcgcca agatcgagaa gccgcaggcg ctcgagcggc tcggcgagat catcgacgcc
      121 gccgacggca ccatggtcgc gcgcggcgac ctcggcgtcg agctcggccc ggagaaggtg
      181 ccgctgtggc agaagcggat catcgaggag accaaccggc gcggcaagat cgtcatcacc
      241 gcgacccaga tgctggagtc gatgatcacc cagccgcggc cgacccgcgc cgaggcgtcc
      301 gacgtcgcca acgccgtgct cgacggcacc gacgccctga tgctgtccgc cgagtccgcg
      361 tcggggctgt tcccggtcga ggcggtccgc accatggcgc ggatcatcga cgagatcgag
      421 cgcagcgcgt attaccgcgc caacgtcgag gtccccgaca tccagctcgc ggtcccgacc
      481 aacgcgatcg cgcacgcggc gatcaccgcc gcccgcgcga tgcggctcaa gaccatcgtc
      541 gtggtgtcgg agtccggcgg cgccgcgcgg ctggtgtccg agtaccgccc ggatgcaaat
      601 atcgtgtgtt tgacaaccaa cgaggtcagc ttccggcggc tcgcgctgtt ctggggggtc
      661 acaccggtcc tgatctcccc ctcagccacc accgaggagc tgatcgaccg cgtcgaggcg
      721 accctgatcg agcgcaacct cgcgttgccg ggcgagaacg tgctgatcac gatggcggtg
      781 ccggtcggct ccgggatgca gaccaacgtc ctgaagattc acgagatcca gcgctagtcg
      841 ccccggcggg gcgagcgcgg gcggacccag gcggaaaagt tgcggcaaac tcgtgctcgg
      901 ggcgcggcat ttcgacgccc tccgcgtcga ggttgagcgc gccgggccag cacgctagtc
      961 tcaggcgcgg tggtggcgac tccacgatcg atcgacgcag gcgttgggat cggcgagatc
     1021 ttcgtcgaca agtatcgcgt cgaccggatc ctcggccacg gcggcatggg cgtggtcgcg
     1081 atgtgcaccc acctcgcgct gaacgagcac gtcgcgatca agatgctgcg caaggacgtg
     1141 ctcgacgacg cggacgcggt cgagcggttc atgcgcgagg cccaggccgc ggtcaagctc
     1201 aagagcgagt acgtcgcgcg ggtgatcgac gtcggccggt cgagcgccaa cgtcccctac
     1261 atggtgatgg agtacctcga cggcgaggac ctcgggcagc tgctcgagga tcgccgcacg
     1321 ctcggcgtgc cgtgggcggt cgagatcacc atgcagtgct gcgaggcgct cgccgaggcc
     1381 cattcgctcg gcatcgtcca ccgcgacgtc aagccgacca acctgttcgt gacctggcgg
     1441 cccgacggca gcgcgatcat caaggtgctc gacttcggga tctccaaggc gctgaccggc
     1501 aactcgatgg cgctgaccca gacccagtcg ctgctcggca cgccggccta catgtcgccc
     1561 gagcagatgc gctcggcgcg catggtcgac gcgcgcaccg acatctggtc gctcggcacc
     1621 gtgctgtacg agatgctcga gggccggcgg ccgttcgagg ccgagagctt ctccgagatg
     1681 tgcgtcaagg tcgcggtcga tgatccgggc gcgatgaccc agacgccgcc cgggctccag
     1741 ctggtcgtgc tgcgctgcct cgagaagtcg ccggaccagc gctacccgtc gatggccgag
     1801 ctcgcgcgcg acctggtgcc gttcgcgcag gatccgcacc aggcgcaggt cctggtcgag
     1861 cgcatgacgc gcacgctgcg ccgcagccag gcccagcccg tcgagtggag cccggcgagc
     1921 agctcgggtt cgatccgcct gccgagcggc gagcgcgtcg ccgagacctc gccggcaccg
     1981 gcggccgcac ccgcgcc
//
LOCUS       DASVOR010001584         6628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_424639,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001584 DASVOR010000000
VERSION     DASVOR010001584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424639"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VF516_29790"
     CDS             <1..640
                     /locus_tag="VF516_29790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111975.1"
                     /translation="DRDFFARQLGLPTPPPPPATELAALAADCHDPLRGEPLPAACRD
                     LIEATLAVDGVCARHISLMVSRTPATFKLDVRLPADRVAPLLRAIGWPGDVRRAVDGI
                     RAVMPWPGELQLNLVLHPALRTSLEVELLTGRGDARREDRLALLDGLVAAGACDAAKA
                     AALRDAWLDPVSTGRDGTVVARSWYVKVRFEGDRIAEAKAYLGLMPRRRAPE"
     gene            682..5655
                     /locus_tag="VF516_29795"
     CDS             682..5655
                     /locus_tag="VF516_29795"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8111976.1"
                     /translation="MTGFDIRESQIIKADPYFQLKRVVTTEGEEFLIKLATEDRGHEE
                     KLLEREFEFLRSFAWGHGLVSVRLTRHRGRLAAIYEPFDGVPLEPRSGAGAPDLAEFA
                     ETAAGICDIFAMLHGAGSLLLGVGPGSFLRSARTRQLLLADAPFAQAQAAPVDRGDYW
                     LESPYLVYAAPELLGDDDRTVDHRADLYALGGLLYHLLAHRPMFDAVEPAEIRHCHLA
                     KKPRHLLELDPSLPRDLAHAVMRLVAKNPLERFTSVEAFERAAAGHSGSPWGRIGAPS
                     ARPGRSVPLTVSFSTALYGNDDALTTVREQIERARAAPAIVFLEGEPGIGKTTLLREL
                     RRLETRVQFCCGDFSGSGAPQPLGGWTSALIDLANAVLTSPGGGLEVWRARILDALGE
                     WAPLIAALAPEWQTILRCPEPASNDPLDTSLNRLALAIRCVLGSFADDESPVVLVLED
                     LQWADAPSLRMLELIVTAPERLNLLVVAAVRGGEAAGGEASAVRALSQRLQAAGSDVS
                     TIRLRPWSQRDALAFITDSLEGDIAEGDVLAELLLAKTHGNPFFIRELLRELLRHNAI
                     RLEPLDSTWRWDRQALRRLPVTDNVIVFLSRRLAGLPEELTHLLRTAACLGREFRLAD
                     VCAVDDRPPDTAERALERAVADGLLIQREGHPIATSYEFVHDRVLEAARALLSDDARA
                     ALHLRIGRALLKQPGADTDEERSYQIASHFNMARHLVTARDERHASAELDLRAGRRAK
                     LRGAFGQALEFLQAGLAFLAEEGSSGAADAAWRDHFQLTLGLHEATAEMALLNGQLTV
                     MNRLCDAILDRVDDPRLKVRAYELRICGLKAEKKFSAAVDAALQILGELGIEFPWRPT
                     TLHNLLGFVATRRRVFAGPVSRLAELPAMVDEETKAAGRIIQSVYPAAYLGRPKLFPF
                     LVYRHVNASLEHGNEDYSGVTYTAFGVVLSGIGQFARAAEIGSVGLDLLRRNNADRLK
                     AQAVMAYYTFIFPWQNHIRDTLPHYKEGLEAGLAHGDFEYASYIMTLESLARLHGGES
                     LDTLQPELERHAAKIKSLGQERSILLQNMLCQMVHTLRHGPERDAPLSGKFYDDAAEL
                     PRCLEPLDANLVFHNYLAKLTLGLFLGDHELALDAARRGRAYLKDGAFSNYLAAVFMA
                     YESLVYLLAAQRGDRSAARMRTVRRNLRRLRTWARAAPMNFANKLHLVEAERCRLRGR
                     GERATEHYEKAIELADTHGFVHEAGLAQQRAASYYLGKGMERLGRQYLRDCYASYRRW
                     GAEAVTRQLEAAHVQPFALLAAGSAGPVRRSANSLLEMLDYRMLLKSSQAISSEFRIP
                     RLLERLLADLREHTGAQRALLVLAKDKVLHVELEAGSEPPGVSSIAGETVDDSERLCR
                     AVVHYCARKVEPVVLVDAALEGLFVRDPYVRAHRPKSVLCTPMHHQSTLIGLVYLENN
                     EVSHVFTDAQLELVNLLVGQAAISIANAQLHAQSLAAHQARINPHFLFNALSSIADLA
                     ISDGRTAEMAIVKLASLYRYILTASVDRLVTLDQELEIVRNYLILEKLRHGAKLDFTF
                     DIEGDTARVRLPGLLIQPLVENSIRHGIVPKLTPGKVRVGAAVRTDRCCIVVEDDGDG
                     AGASTPGTGFGQRSVLERLEIAYGKGFSFAIDRSGGYRVEIEIPAGE"
     gene            5799..6392
                     /locus_tag="VF516_29800"
     CDS             5799..6392
                     /locus_tag="VF516_29800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8111977.1"
                     /translation="MTLADARDASPEVDRDSDVRDLVRRGEMQAALCRLMRRHGRPIY
                     RYCCEQLRDPELAADVHQQVFIAAFRDLEKFRGRGTLRSWLFGIAYHRVIDAARARAR
                     SLAWISVTEAAKVADPRPPVDQACDDERLHAALVAELADLPAHVRSAILLHYQQGMSF
                     ESMARISGEKAGTLSARVMRAMPVLRERIEARLRRPR"
     gene            complement(6408..>6628)
                     /locus_tag="VF516_29805"
     CDS             complement(6408..>6628)
                     /locus_tag="VF516_29805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111978.1"
                     /translation="RSYQPPARSYQPPARSYQPPARSYQPPARSSPPAHTGGSSSSSS
                     HSSSSSSSSHSSSSSSSHSSSSSSGHHR"
BASE COUNT          936 a         2303 c         2382 g         1007 t
ORIGIN      
        1 cgatcgcgat ttctttgcgc gccagctcgg actcccgacg ccgccgccac cgccggcgac
       61 cgagctggcg gcgctggcgg cggactgcca cgatcccctg cgcggcgagc cgctaccggc
      121 agcgtgtcgc gacctgatcg aggcgacgct ggccgtcgac ggtgtctgcg cgcgccacat
      181 ctcgctcatg gtgtcgagga cgccggcgac gttcaagctc gatgtccggc tcccggccga
      241 tcgcgtcgcg ccgctgctgc gcgcgatcgg ctggccgggt gacgtgcgcc gcgcggtgga
      301 cggcattcgc gcggtgatgc cgtggcccgg tgagctgcag ctgaacctcg tgcttcaccc
      361 ggcgctgagg acgagcctcg aggtcgagct cctgaccggt cgcggcgacg ctcgtcgcga
      421 ggatcggctc gccctgctcg acgggctcgt cgccgccggg gcgtgcgatg ccgccaaggc
      481 agccgccctg cgcgacgcat ggctcgatcc ggtgagcacg ggtcgcgacg gcacggtcgt
      541 ggcgcggagc tggtacgtca aggttcgttt cgagggcgac cgtatcgcgg aggccaaggc
      601 gtatctcggg ctgatgccgc ggcgtcgcgc gccggagtag cggcgtgggc gcgaccaaca
      661 cgggatccat gcgagtggca gatgacaggt ttcgatatca gagagtccca gatcatcaag
      721 gctgatccgt actttcagct gaagcgcgtg gtcacgacgg agggcgagga gttcctgatc
      781 aagcttgcga ccgaggaccg cggccacgag gagaagctgc tcgagcgcga gttcgagttc
      841 ctgcggtcgt tcgcctgggg ccatgggctg gtgtcggtcc gcctgacacg gcatcgcggc
      901 cggctcgccg cgatctacga gccgttcgac ggggtgccgc tcgagccgcg ctccggcgcc
      961 ggtgcgcccg atctcgcgga gttcgcggag accgcggcgg gcatctgcga catcttcgcg
     1021 atgctgcacg gcgcgggatc gctgctgctc ggggtggggc cgggctcgtt cctgcgcagc
     1081 gcgcggaccc ggcaactgct gctggccgac gcgccgttcg cgcaagcgca ggcagcgccg
     1141 gtggatcgcg gcgattactg gctcgagagc ccgtacctgg tctacgcggc gccggagctg
     1201 ctcggcgacg atgatcggac ggtggaccat cgcgccgacc tctacgcgct gggcggtctg
     1261 ctctaccacc tgctggcgca ccggccgatg ttcgatgcgg tcgagccggc ggagatccgg
     1321 cattgccacc tcgccaagaa acccaggcac ctgctcgagc tggacccgtc gctgccccgg
     1381 gatctggcgc acgcggtgat gcggctcgtg gccaagaacc cgctcgagcg gttcaccagc
     1441 gtcgaggcgt tcgagcgggc ggcggccggt cattccggat cgccctgggg ccgcatcggc
     1501 gcaccgtcgg cgcggcccgg ccggtccgtg ccgctgaccg tgtcgttcag caccgcgctg
     1561 tacggcaacg acgatgcgct gaccaccgtg cgcgagcaga tcgagcgcgc ccgggccgcc
     1621 cctgcgatcg tcttcctcga gggcgagcca ggcatcggca agaccacgct gctgcgcgag
     1681 ctgcggcggc tggagacccg tgtccagttc tgctgcggcg acttcagcgg cagcggtgca
     1741 ccgcaaccgc tgggcggctg gacgtcggcg ctgatcgatc tggccaacgc ggtgctcacc
     1801 agcccgggcg gcgggctcga ggtgtggcga gcgcggatcc tcgatgcgct cggcgagtgg
     1861 gccccgctga tcgccgcgct cgccccggaa tggcagacca tcctgcgctg cccggagccg
     1921 gcgagcaacg atccgctcga caccagcctg aaccggctgg cgctggcgat tcgctgcgtg
     1981 ctcgggagct ttgccgacga cgagtcgccc gtcgtgctgg tgctggagga tctgcagtgg
     2041 gccgatgcgc cgtcgctgcg catgctggag ctcatcgtca cggcgcccga gcggttgaac
     2101 ctgctggtgg tcgcggcggt gcgcggcggc gaggccgccg gcggcgaggc ctcggcggta
     2161 cgggcgctga gccagcgctt gcaggccgcg gggagcgacg tgtccacgat ccggctccgg
     2221 ccgtggtcgc agcgcgacgc gctcgccttc atcaccgaca gcctcgaggg cgacatcgcc
     2281 gagggcgacg tgctcgccga gctcctgctc gccaagaccc acggcaatcc gttcttcatc
     2341 cgcgagctgc tgcgcgagct gctgcggcac aacgcgatcc gcctcgagcc gctcgacagc
     2401 acgtggcgct gggaccgcca ggcgcttcgc cgcctgcccg tgaccgacaa cgtgatcgtg
     2461 ttcctgtcgc gccggctcgc cgggctcccc gaggagctga cccatctgct gcggacggcc
     2521 gcgtgcctgg gccgcgagtt ccggctggcg gacgtctgcg cggtcgacga tcggccgccg
     2581 gacaccgcgg agcgcgcgct cgagcgcgcg gtcgccgacg gtctgctgat ccagcgcgaa
     2641 ggccacccga tcgcgaccag ctacgagttc gtccacgatc gcgtgctcga ggcggcgcgc
     2701 gcgctgctgt cggacgacgc gcgggccgcc ctgcacctgc ggatcggccg cgcgctgctg
     2761 aagcagcccg gggcggacac cgacgaggaa cgcagctacc agatcgccag ccacttcaac
     2821 atggcgcgcc acctggtgac cgcccgcgac gaacgccacg cgagcgcgga gctcgacctg
     2881 cgcgcaggcc ggcgggccaa gctgcgcggc gcgttcggcc aggcgctcga gttcctgcag
     2941 gccgggctgg cgttcctcgc cgaggaaggc agctccggcg cggccgacgc cgcatggcgc
     3001 gaccacttcc agctgacgct cggcctccac gaggcgaccg ccgagatggc gctgctcaac
     3061 ggtcagctga ccgtgatgaa ccggctgtgc gacgcgatcc tggaccgcgt cgacgacccc
     3121 cggctcaagg tgcgggccta cgagctgcgg atctgcggcc tgaaggccga gaagaagttc
     3181 tccgcggccg tcgatgcggc gctccagatc ctcggcgagc tcggcatcga gtttccgtgg
     3241 cggccgacca cgctccacaa cctgctcggg ttcgtcgcga cccgccggcg ggtgttcgcc
     3301 ggcccggtgt cccggctggc cgagctgccc gcgatggtcg acgaggagac caaggcagcg
     3361 gggcggatca tccagtcggt gtatccggcc gcctacctcg gccgcccgaa gctgtttccg
     3421 ttcctggtct atcgccacgt caacgcatcg ctcgaacacg gcaacgagga ctactccggc
     3481 gtcacctaca ccgcgtttgg cgtcgtcctg agcggcatcg gacagttcgc gcgcgcggcc
     3541 gagatcggca gcgtcggcct cgacctgctg cgccgcaaca acgccgaccg gctcaaggcg
     3601 caggcggtca tggcgtacta cacgttcatc ttcccgtggc agaatcatat ccgggacaca
     3661 ctgccgcact acaaggaagg cctggaggcg gggctcgcgc acggcgactt cgagtatgcg
     3721 tcgtacatca tgacactcga gtcactcgcc cgcctgcatg gcggcgagtc gctggatacg
     3781 ctgcagccgg agctcgagcg ccacgccgcc aagatcaagt cgctcggcca ggagcgatcg
     3841 atcctgctcc agaacatgct gtgccagatg gtgcacaccc tgcgccacgg gccggagcga
     3901 gacgcgccgc tgtcgggcaa gttctacgac gatgccgcgg agctgccgcg ctgtctcgag
     3961 ccgctcgacg cgaacctggt atttcacaat tacctcgcca agctcacgct cgggctgttc
     4021 ctgggcgatc acgagctggc gctcgacgcc gcccgccgcg gccgcgccta cctgaaggac
     4081 ggggcattct ccaactatct cgccgcggtg ttcatggcgt acgagtcgct ggtgtacctg
     4141 ctcgcggcgc agcgcggcga tcgctcggcg gcgcggatgc ggacggttcg ccgcaacctg
     4201 cgccggttgc ggacctgggc cagggcggcg ccgatgaact tcgcgaacaa gctgcatctc
     4261 gtcgaggccg agcgctgccg gttgcggggg cgcggcgagc gcgccaccga acactacgag
     4321 aaggcgatcg agctcgccga cacccacggc ttcgtccacg aggccggcct cgcccagcaa
     4381 cgcgcggcct cgtactacct cggcaagggg atggagcgcc tcggccggca gtatctgcgc
     4441 gactgctacg catcgtatcg ccgctggggg gccgaggcgg tgacccggca gctcgaggcc
     4501 gcacacgtcc agccgttcgc gctgctcgcc gccggcagcg ctggcccggt ccgccgcagc
     4561 gcgaacagcc tgctcgagat gctcgactac cgcatgctgc tcaagtcgtc gcaggcgatc
     4621 tcgagcgagt tccggatccc gcgcctgctc gagcggctgc tcgccgatct gcgcgagcat
     4681 accggcgcgc agcgggcgct gctcgtgctg gccaaggaca aggtcctgca cgtcgagctc
     4741 gaggccggca gcgagccacc gggggtctcg agcatcgccg gggagacggt ggacgacagc
     4801 gagcggctgt gccgcgcggt cgtgcattac tgcgcgcgca aggtcgagcc ggtggtgctg
     4861 gtcgacgcgg cgctcgaggg actgttcgtg cgcgacccgt acgtgcgggc gcaccgaccc
     4921 aagtccgtgc tgtgcacgcc gatgcaccac cagagcacgc tgatcgggct ggtctacctc
     4981 gagaacaacg aggtgagcca cgtgttcacg gacgcgcagc tcgagctcgt gaacctgctc
     5041 gtgggccagg ccgcgatctc gatcgcgaac gcgcagctgc acgcgcagtc cctcgcggcg
     5101 caccaggccc ggatcaaccc gcacttcctg ttcaacgcac tgagctcgat cgccgatctg
     5161 gcgatctccg atggtcggac cgccgagatg gcgatcgtga agctggcgag cctgtatcgc
     5221 tacatcctca cggcctcggt cgaccggctg gtcacgctgg accaggagct cgagatcgtc
     5281 agaaattatc tgatcctgga aaagctgcgc cacggggcca agctcgactt cacgttcgac
     5341 atcgagggcg acacggcgcg cgtgcggctg cccggcctgc tgatccagcc gctggtcgag
     5401 aacagcatcc gccacgggat cgtgccgaag ctgaccccgg gcaaggtccg ggtgggcgcc
     5461 gcggtccgca cggatcgctg ctgcatcgtg gtcgaggacg acggcgacgg ggccggcgcc
     5521 agcacgccgg gcaccgggtt cggccagcgc agtgtcctcg agcggctgga gatcgcgtac
     5581 ggcaaggggt tctcgttcgc gatcgaccgc agcggtggtt accgggtgga gatcgagatt
     5641 ccggccggtg agtagggcgg cctgggccgc catccattgc cgatccgcga ccggctgtcg
     5701 gctcgctccg acgcttggtc tggagccagc tgcacgggga ggtgcgctcg cctatagatg
     5761 ggtcatccgc catgatgacc tcggcgatac cgttcgacat gaccctggcc gacgcgcgag
     5821 acgcgtcgcc cgaagtggac cgggacagcg acgtgcgcga cctcgtgcgc cgcggcgaga
     5881 tgcaggccgc gctgtgccgc ttgatgcggc gccacgggag accgatctat cgctactgct
     5941 gtgaacagct gcgcgatccg gagctcgccg cggatgtgca ccagcaagtg ttcattgctg
     6001 cgttccgcga tctcgaaaag ttccgcggtc gcggaacgct gcgatcgtgg ctgttcggca
     6061 tcgcgtacca ccgcgtcatc gatgcagcgc gagcgcgcgc tcggtcgctg gcatggatct
     6121 cggtcaccga ggccgcgaag gtcgccgacc cgcggcctcc ggtggaccag gcatgcgatg
     6181 acgagcggct ccatgccgcg ctcgtcgccg agctcgcgga cctccccgcg cacgtgcgca
     6241 gtgcgatcct gctgcactat cagcagggca tgtccttcga gtccatggcc cggatctccg
     6301 gtgagaaggc gggcacgctg tcggctcggg tcatgcgtgc gatgccggtg ctgcgcgagc
     6361 gcatcgaggc gcggctccgc cgtccgcgct gatcggccgg gctccggtca gcggtggtgg
     6421 ccgctgctgc tgctgctgct gtgcgagctg ctcgacgagc tcgagtgcga gctgctgctc
     6481 gacgagctcg agtgcgagct gctgctcgac gagccgccgg tatgcgccgg cgggctgctg
     6541 cgcgccgggg gctggtagct gcgcgccggg ggctggtagc tgcgcgccgg gggctggtag
     6601 ctgcgcgccg ggggctggta gctgcgcg
//
LOCUS       DASVOR010001585         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_454350,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001585 DASVOR010000000
VERSION     DASVOR010001585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454350"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VF516_29810"
     CDS             <1..1163
                     /locus_tag="VF516_29810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111979.1"
                     /translation="DRLVGINNCFKQATEFLLQHLPTTVDTPPPGGPELDLGSVAGAL
                     VARGGALDPDHTVPRDVVGLVPSPRPDPTVDPLRPRAATPRFPQAMSAPLLAIDREML
                     LPGVDRIATDSVGVLVGNPRFIEAYMVGLNHELSRELLWRGLPADLAATFADRFWDMR
                     GNAGRTGAPAPQLPPIATWQGGLGANATGVGATGMLVLIVRGRLLQRYPHTAIYAARA
                     VPKLDANDQPIVAPDGTPIPAPGPEERYPEFRGTIDPDITYLGFQLGVDEARGGNGAL
                     GWFFILQEQPTAPRFGLDEPGDGPQEAPTSWSDLDWGDVVPPGTDLAAVRYVRVAGPL
                     AAQPVTLPVLAGHAQPTATWGADASQMAAITYQRPMRIAIHARTALPATGAP"
     gene            1163..>2137
                     /locus_tag="VF516_29815"
     CDS             1163..>2137
                     /locus_tag="VF516_29815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111980.1"
                     /translation="MTAPTLRQIAVPERPRESPRALPGDELVASGEAFGALDAKSAVL
                     INAVNGAAVSAAIVDWRDTQIRFRCPDGVGAPGPKEVRIRTRQGTSSAVTVALEDLPR
                     LSAAPVALLPLGLQTRFMADGRELWIRAIPDTVHVDSHDERLTAEEAELGRRYRDAGA
                     GRDDVWLDLTTRFGAPRAEWIVRTTLAGTVTLRDQPWARAARSRLLPRRLYASAHDDA
                     GALIATGYGHPIPFELPLGPDPGGAADADPTRDPGIAWMTDFSVALARGMAIKLQLPA
                     PPPTRIARLVVLGVETAITAEQGAGELGDALAAHRYTRGIGFLPEGTPT"
BASE COUNT          273 a          822 c          764 g          278 t
ORIGIN      
        1 ccgatcgcct ggtcgggatc aacaactgct tcaagcaggc gacggagttc ctcctgcagc
       61 acctgccgac gaccgtcgac acgccgccgc cgggagggcc ggagctcgac ctgggctcgg
      121 tcgccggtgc gctggtggcg cgcgggggcg cgctggatcc cgaccacacc gtgccccgcg
      181 acgtggtcgg gctggtcccg tccccgcggc ccgatccgac ggtcgatccg ctccggccgc
      241 gcgccgcgac gccgcggttt ccccaggcga tgagcgcgcc gctgctggcg atcgatcgcg
      301 agatgctgct gcccggcgtc gaccgcatcg cgaccgacag cgtcggggtg ctcgtcggca
      361 acccgcgctt catcgaggcg tacatggtcg gtctcaacca cgagctcagc cgcgagctgc
      421 tctggcgcgg actcccggcg gatctcgcgg cgacgttcgc cgaccggttc tgggacatgc
      481 gcgggaatgc cggacggacc ggcgcaccgg cgccgcagct gccgccgatc gccacgtggc
      541 aaggcggcct gggagcgaac gcgaccggcg tgggcgcgac cggcatgctg gtcctgatcg
      601 ttcgcggccg gctcctgcag cgctatccgc acaccgcgat ctacgcggcc agggcggtcc
      661 cgaagctcga cgcgaacgac cagccgatcg tggcgccgga cggcacgccg atccccgcgc
      721 ccggccccga ggagcgctac cccgagttcc gcggcacgat cgatcccgat atcacctacc
      781 tgggcttcca gctcggcgtc gacgaggcgc gcggtggcaa cggcgcgctg ggctggttct
      841 tcatcctcca ggagcagccc accgcgcccc ggttcggact cgacgagccc ggcgatggcc
      901 cccaggaggc gcccaccagc tggtcggatc tggactgggg ggacgtcgtg ccacccggca
      961 ccgatctcgc ggcggtgcgc tacgtccgcg tcgccgggcc gctggccgct cagcccgtga
     1021 cgctgccggt gctcgccggg catgcccagc cgacggccac ctggggcgca gacgcctcgc
     1081 agatggcggc gatcacctat caacgtccga tgcgcatcgc catccatgcc cgcacggcgc
     1141 tgcccgcaac cggagcaccc tgatgacggc gcccacgctt cgccagatcg ccgtcccgga
     1201 gcgcccccgc gagtcgcccc gcgcgctgcc gggcgacgag ctggtggcgt ccggtgaggc
     1261 attcggcgcg ctggatgcca agtcggcggt gctgatcaac gcggtgaacg gcgcggcggt
     1321 gtccgctgcg atcgtcgact ggcgcgacac gcagatccgc ttccggtgtc cggacggcgt
     1381 tggcgcgccg gggcccaagg aggtgcgcat ccgcacgcgc cagggcacga gcagcgctgt
     1441 caccgtcgcg ctggaagacc tgccgcggct gtcggccgcg cccgtggcgc tcctgccgct
     1501 cgggctccag acccgcttca tggccgatgg ccgggagctg tggatccgcg cgatcccgga
     1561 taccgttcac gtcgacagcc acgacgagcg cctcacggcc gaggaggccg agctcggccg
     1621 gcgctaccgc gacgcgggcg ccggacgcga cgatgtctgg ctcgacctga cgacgcggtt
     1681 tggcgcgcca cgcgccgagt ggatcgtccg tacgacgctg gccggcaccg tcacgctgcg
     1741 cgatcagccg tgggcgcgcg cggcccgcag ccgcctcctg ccccggcggc tgtacgcatc
     1801 tgcccacgac gacgcgggcg cgctgatcgc gacgggctat ggccacccga tcccgttcga
     1861 gctgccgctc ggccccgatc ccggcggcgc ggccgatgcc gatccgacgc gcgaccccgg
     1921 catcgcatgg atgaccgact tctcggtcgc gctcgcgcgc ggcatggcga tcaagctgca
     1981 gctgcccgct ccgcccccga cccggatcgc gcggctggtc gtgctcggcg tcgagaccgc
     2041 gatcaccgcc gagcagggcg cgggcgagct gggcgacgcg ctcgcggccc accgctacac
     2101 gcggggcatc gggttccttc cggagggcac gccgacc
//
LOCUS       DASVOR010001586         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395437,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001586 DASVOR010000000
VERSION     DASVOR010001586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395437"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            10..>1827
                     /locus_tag="VF516_29820"
     CDS             10..>1827
                     /locus_tag="VF516_29820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111981.1"
                     /translation="MSGAVPDELTTAHHAGTPLAERTERVDAARQELLQAGADPAVVL
                     ALSTGQLLQIDQLAGDVLQRWLRFTAGEHLAWQNGLSVDENLRLFRSTDRTEIAHLVL
                     EGLRRIAAPARELKLALYHGDIASVPNVDGYAVTVTPGVPTAGAAAALSARVTPTGAA
                     SIASSVVEAIEVSNLPGTHVLVAPLELPPVGRAVDAATVERLARDVARTADRRGLASI
                     ATPAFATGLGMSFQDSVPAMIAGFRAGRGRLPSTLVFCETDPARYELLRSALGPQAVE
                     LRAGPPSFRSTRRAVLNIEVDEPRPSSAGRVSCALYLVGGRASTADRRRADGDQVIAP
                     RKDVSLSWATWQALRQPVRDFEGSLRVGRTLWRELLAEDIRVPLIDLGDRSLAVLGAG
                     TASELPWELMMEDRDGAMPLAGGIVRRSVLAAPYRPGARGPGRSGLRVLIAMDMRGDE
                     KSSTAEFEAVVASLGGRADVHVTVVADPDAAVIALANGCDIFHHIGDVSDAGRLLVDS
                     IEAPVERSWIPPGLVYLSAYRPARTVDPRPPASFVYGAAIKSAEAFLHAEVSSVIVAL
                     DVDEASACRFVSTAYARLAAGHRLGTAVRAARGELFRTRS"
BASE COUNT          228 a          667 c          667 g          265 t
ORIGIN      
        1 tcgatgctgg tgtccggtgc cgtcccagac gagctcacca ccgcgcacca tgccggaacc
       61 ccgctcgccg agcggaccga gcgcgtcgac gccgcgcgcc aggagctcct gcaagccggc
      121 gccgaccccg cggtggtgct cgcgctctcg acggggcagc tcctccagat cgaccagctg
      181 gccggcgacg tgctccagcg ctggctgcgg ttcacggccg gcgagcacct ggcatggcag
      241 aacggcctga gtgtcgacga gaacctgcgt ctgttccgct cgaccgatcg aaccgagatc
      301 gcccacctgg tgctcgaggg gttgcgccgg atcgcggcgc cggctcgcga gctcaagctc
      361 gcgctctatc acggcgacat tgcctcggtg ccaaacgtag atggctacgc ggtgacggtc
      421 acgccggggg tccccaccgc cggcgccgcc gcggcgctgt ccgcccgggt gactcccacc
      481 ggcgctgcat cgatcgcgtc gagcgtggtc gaggcgatcg aggtgagcaa cctccctgga
      541 acccacgtgc tggtcgcccc cctcgagcta ccgccggtcg ggcgagcggt cgacgccgcg
      601 accgtcgagc gcctggcgcg cgacgtggca cggaccgcgg accggcgggg gctggcgtcg
      661 atcgcgaccc cggcgttcgc gaccggcctc gggatgtcgt tccaggactc ggtgccggcg
      721 atgatcgccg ggtttcgcgc cggccggggc cgcctgccat cgacgctcgt gttctgcgag
      781 accgatcctg cgcgctacga gctgctgcgc agcgcgctcg gtccgcaggc ggtcgagctc
      841 cgcgccgggc caccgtcgtt ccgcagcacg cgccgcgccg tgctgaacat cgaggtcgac
      901 gagccgcgtc cgagcagcgc cgggcgcgtg agctgtgcgc tgtacctggt cggcgggcga
      961 gcgagtaccg ccgaccgccg ccgcgccgac ggcgatcagg tgatcgcgcc gcgcaaggac
     1021 gtctcgctgt cgtgggcgac ctggcaggcg ctgcgccagc cggtgcgcga cttcgagggc
     1081 tcgctgcgcg tcggccgtac gctgtggcgc gagctgctcg ccgaggacat ccgcgtgccg
     1141 ctcatcgatc tcggggaccg atcgctcgcc gtgcttggcg ccggcaccgc gtccgagctg
     1201 ccgtgggagc tgatgatgga ggaccgcgac ggcgcgatgc cgctcgcggg cgggatcgtg
     1261 cggcggagcg tgctggcggc gccgtaccgt cctggcgcgc gtggcccggg acgttcgggt
     1321 ctgcgcgtcc tcatcgccat ggacatgcgc ggcgatgaga aatcgagcac agcagagttc
     1381 gaggccgtcg tggcgtctct gggcgggcgc gccgacgtcc acgtcacggt ggtcgccgac
     1441 cccgatgcgg ccgtgatcgc gctcgcgaac ggctgcgaca tctttcacca catcggagat
     1501 gtcagcgacg ctggccgcct gcttgtcgac agcatcgagg cgcccgtcga gcgaagctgg
     1561 atcccgcccg ggctggttta cctgagcgcc taccggcccg cgcggaccgt cgaccccagg
     1621 ccgccggcat cgttcgtgta tggcgccgcg ataaagtcgg cagaagcgtt cctgcacgcc
     1681 gaggtgagct cggtgatcgt ggcgctcgac gtcgacgagg ccagcgcatg ccgcttcgtc
     1741 tcgaccgcgt acgctcgcct cgccgccggc caccggctcg gcacggcggt gcgcgccgcc
     1801 cgcggcgagc tgttccggac ccgcagc
//
LOCUS       DASVOR010001587         1982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_17199,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001587 DASVOR010000000
VERSION     DASVOR010001587.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1982
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17199"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF516_29825"
     CDS             <1..232
                     /locus_tag="VF516_29825"
                     /inference="COORDINATES: protein motif:HMM:NF012379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEX8111982.1"
                     /translation="GELTDPVEQRARFEHDQATRRARGKPVYPIDERLLAALAEGLPP
                     SAGIALGVDRLIMLATGAETIDEVLAFAAGEL"
     gene            260..568
                     /locus_tag="VF516_29830"
     CDS             260..568
                     /locus_tag="VF516_29830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111983.1"
                     /translation="MLAARSLLNLHFKGLGPADILAAEALAIDRTNVLARVINTELTH
                     EISPDDARATAAAHPDDWRAWRLVVHAVKDRAEAEAALGRVCALTGNDAPECVLRAAR
                     "
     gene            complement(571..987)
                     /locus_tag="VF516_29835"
     CDS             complement(571..987)
                     /locus_tag="VF516_29835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8111984.1"
                     /translation="MDDHALNILLVDDDEVDVMTVKRAFSRANIINKLYVATDGIEAL
                     HLLRGDGIPPSRRLVLLDLNMPRMNGIELLREIRADPALHALTVVVLTTSNEDRDRVE
                     AYQLNVAGYLLKPVTFHAFAEVMSTLNKYWTLMEMP"
     gene            complement(980..>1982)
                     /locus_tag="VF516_29840"
     CDS             complement(980..>1982)
                     /locus_tag="VF516_29840"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8111985.1"
                     /translation="SGAPGLLELAARTLQQPRIVRIADLPRDRASPPPPGLAVASYLA
                     VPVIGRSGCTIGALAFGHATPGVFTEETERLIAGVAATAAIAMDNARLFAEARELIAA
                     LESSNRELDQFAYVASHDLKAPLRGIANLAQWIEDDLGDRLDDQTRSHLHLLRGRVVR
                     LENLIGGILAYSRAGRDSKDQVSVDVGALVAEVWELLAPPATAHLVVATPLPQLFTSR
                     VQLQQILLNLIGNAIKYNADRELTITVAAERRGRRWQFSVADDGVGIAREFADKIWGL
                     FQTLERRDKVESTGIGLSVVRKIVECHGGKAWVESELGRGATFRFTWPADPEEAPHG"
BASE COUNT          267 a          681 c          743 g          291 t
ORIGIN      
        1 cggcgagctg accgatccgg tcgagcagcg cgcgcggttc gagcacgacc aggcgacccg
       61 ccgcgcccgc ggcaagccgg tctacccgat cgacgagcgg ctgctcgccg cgctcgccga
      121 gggcctgccg ccgtcggccg gcatcgcgct cggcgtcgac cggctgatca tgctggcgac
      181 cggcgccgag accatcgacg aggtcctggc gttcgcggcc ggcgagcttt gagttaccct
      241 gcgcggccat gaggaccggt tgctcgcggc ccgcagcctg ctgaacctgc acttcaaggg
      301 cctggggccg gcggacatct tggccgccga ggccctggcg atcgatcgca ccaacgtgct
      361 cgcgcgcgtg atcaacaccg agctcacgca cgagatctcg cccgacgacg cccgcgcgac
      421 cgccgccgcg caccccgacg actggcgcgc ctggcggctg gtcgtgcacg cggtgaagga
      481 tcgcgccgaa gccgaggcgg cgctcggccg ggtgtgcgcg ctcaccggca acgacgcgcc
      541 cgagtgcgtg ctccgagcgg cgcggtagcg ctacggcatc tccatcagcg tccagtactt
      601 gttgagcgtg ctcatcacct cggcgaacgc gtggaacgtg accggcttga gcaggtagcc
      661 ggcgacgttg agctggtagg cctcgacccg gtcgcggtcc tcgttggacg tggtcagcac
      721 gacgaccgtg agcgcgtgca gcgcggggtc ggcgcggatc tcgcgcagta gctcgatccc
      781 gttcatccgc ggcatgttga ggtcgagcag cacgagccgg cgcgacggcg ggatgccatc
      841 gccgcgcagc aggtggagcg cctcgatgcc gtcggtggcg acgtagagct tgttgatgat
      901 gttggccctc gagaacgcac gcttgacggt catgacgtcg acctcgtcgt cgtcgaccag
      961 caggatgttg agcgcgtgat catccatggg gtgcttcctc ggggtcggcc ggccaggtga
     1021 accggaacgt ggcgccgcgg ccgagctcgg actcgaccca cgccttgccg ccgtggcact
     1081 cgacgatctt gcggaccacc gacagcccga tgccggtgct ctcgaccttg tcgcggcgct
     1141 ccagggtctg gaacaggccc cagatcttgt cggcgaactc gcgcgcgatg ccgacgccgt
     1201 cgtcggcgac cgagaactgc cagcgccggc cgcggcgctc ggcggcgacg gtgatcgtga
     1261 gctcgcggtc ggcgttgtac ttgatggcgt tgccgatcag gttgagcagg atctgctgga
     1321 gctggacccg cgaggtgaac agctggggca gcggcgtggc gaccaccagg tgcgcggtgg
     1381 cgggcggtgc gagcagctcc cagacctcgg cgaccagcgc gccgacgtcg acgctgacct
     1441 ggtccttgct gtcgcggccg gcgcggctgt acgcgaggat gccgccgatc aggttctcga
     1501 gccggacgac ccggccgcgc agcaggtgga ggtggctgcg ggtctggtcg tcgagccggt
     1561 cgccgaggtc gtcctcgatc cactgcgcca ggttggcgat gccgcgcagc ggcgccttga
     1621 ggtcgtggct ggcgacgtag gcgaactggt cgagctcgcg gttggagctc tcgagcgcgg
     1681 cgatcagctc gcgggcctcc gcgaacagcc gggcgttgtc catcgcgatc gcggcggtcg
     1741 ccgcgacgcc ggcgatcagc cgctcggtct cctcggtgaa cacccccggc gtggcgtgac
     1801 cgaacgccag cgcgccgatc gtgcagccgc tgcggccgat caccggcacg gcgaggtagc
     1861 tcgccaccgc gagcccgggg ggcggtggcg atgcccggtc gcgcggcagg tcggcgatcc
     1921 ggacgatccg cggctgctgc agggtgcgcg cggcgagctc gagcagtccg ggcgcgccgg
     1981 at
//
LOCUS       DASVOR010001588         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_366922,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001588 DASVOR010000000
VERSION     DASVOR010001588.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_366922"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VF516_29845"
     CDS             complement(<1..814)
                     /locus_tag="VF516_29845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111986.1"
                     /translation="MCVATGHSVAPILRDISVHTRLLIGIPLLIQAERILEQRCRSAV
                     EQLYAGRFADRAVVDRIIDRARRLRDSRAIVLVIAAIAVGGGQALLWGFSELTGPFAG
                     ITEPGELTFARVWYVFVAWPIAQFLLLHWLWHWSIWSYVTVHLSRLPLATIATHPDRA
                     AGIGFLREPINGFSGFVLALSALMASGWWMQILHRRVTLQAFVPEFAAFVILAAIFAC
                     GPLLLFIGMLYRARHREIDRYNELALDYVRAFHRKWIEQRSDRDEILGTADVQ"
     gene            1262..1651
                     /locus_tag="VF516_29850"
     CDS             1262..1651
                     /locus_tag="VF516_29850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111987.1"
                     /translation="MKKMWISGGIVAAVLLSGAGAYAGYKASIGAPSISSTFAVGVMG
                     AARATSDSNSYAGCSLLNSGTTTLGFCTVADATGATKTCSFSNPAMAQAVASITPDSA
                     IRLFFDASGNCTQLYVDTYSFYLPATP"
BASE COUNT          291 a          599 c          596 g          242 t
ORIGIN      
        1 tctggacatc ggcggtgccg aggatctcgt cgcgatcgga ccgctgctcg atccacttgc
       61 ggtggaacgc ccggacgtag tcgagggcca gctcgttgta gcggtcgatc tcgcggtgcc
      121 gtgcgcgata cagcatgccg atgaacagca gcaggggacc gcacgcgaag atcgccgcca
      181 ggatcacgaa cgcggcgaac tcgggcacga acgcctgcag cgtgacgcgc cggtggagga
      241 tctgcatcca ccagcccgat gccatcaagg cggagagcgc gaggacgaag cccgagaatc
      301 cgttgatcgg ttccctgagg aagccgatgc ccgcggcgcg gtccggatgg gtcgcgatcg
      361 tggcgagcgg cagccgcgac aggtgcacgg tgacatacga ccagatcgac cagtgccaca
      421 gccagtgcag gagcaggaac tgcgcgatcg gccaggccac gaacacgtac cacacgcggg
      481 cgaacgtgag ctcgccgggc tcggtgatcc cggcgaacgg cccggtcagc tcgctgaacc
      541 cccacagcag ggcctgtcct cccccgaccg cgatcgccgc gatcacgagc acgatggcgc
      601 gagagtcgcg caggcgcctg gcgcgatcga tgatgcgatc gaccaccgcg cggtcggcga
      661 accgtcccgc gtagagctgc tcgacggcgc tgcggcagcg ctgctcgagg atccgctcgg
      721 cctggatcaa cagcgggatc ccgatcagca accgggtgtg gaccgagatg tcccgcagga
      781 tcggcgcgac ggaatgaccg gtggccacgc acaccagcgc gccgagcatc atcgggcccc
      841 agacgaccgc gacgagcggc catgcgcgcg cactaccatc tgcgcgtgtg aggtggagtc
      901 ggcgcagcag gcgcagaaac ggtccttgcg tgatcgagaa tggctccggt gcgtcgcgcc
      961 cgcgttccgg ctcggagctc accggcgtct ccggcccatg tcgtgcctcc gtaccatgtc
     1021 gtgctctgtc atggtgcttg tcctcggtcc agcttggtct cgcccggacc aggcaccacc
     1081 gccgctcacg gcggtatcac cggagccacg ccggcgggcg ccgggcgatc gtgcgccggg
     1141 ccgatcgcgc gcccgcgcca cgaccctcga aaaaaaaaac cgcggcgccg cctgtcacag
     1201 accaccgggc gccggatcta cccgttcggt ccaggagctg gaccggaaca ggagttctcg
     1261 gatgaagaag atgtggatca gtggaggtat cgtggccgcg gtgctgctca gcggcgccgg
     1321 cgcctacgca gggtacaagg ccagcatcgg tgcgccgtcg atcagctcga cgttcgccgt
     1381 cggtgtcatg ggcgcggcgc gcgcgacgtc ggatagcaac tcgtacgccg gctgctcgct
     1441 gctcaactcg ggcaccacca cgctcggctt ctgcacggtc gccgacgcca cgggcgccac
     1501 gaagacgtgc agcttcagca accctgcgat ggcccaggcg gtcgcgtcga tcacaccgga
     1561 ctccgcgatc cggctgttct tcgacgccag cggcaactgc acgcagctct acgtcgacac
     1621 ctactccttc tacctgccgg cgacgccgtg acggccgcgc accacctgac ccgggccggg
     1681 atgctgctgg tgttcggcct cggctgcgcg gggctggggg cgcggtgg
//
LOCUS       DASVOR010001589         1804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395469,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001589 DASVOR010000000
VERSION     DASVOR010001589.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1804
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395469"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1804)
                     /locus_tag="VF516_29855"
     CDS             complement(<1..>1804)
                     /locus_tag="VF516_29855"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEX8111988.1"
                     /translation="VVVITAGFAEVGGLAAEQRLRELVRGAGMRLVGPNCMGVMSTDP
                     AVRLDATFSPIFPPAGNVSMLSQSGALGLAMLDYAKELHLGIADFVSIGNKADVSVND
                     LLAYWHSSPRTRVIALYLESFGNPRMFARLAPEVARERPIVAVKSGRSAAGTRAASSH
                     SAALASLDVGIDALFAQTGVIRTTTLEEMFDVVALLSSQPVPAGRRIGVVTNAGGPGI
                     LLADACEAHGLTLSPFAEATAAALRGFLPAQASVANPVDMIASAPPEHYERAIELVGR
                     DPNVDAVVAIYVPPLVTQPEAVAAAIARGAGTLPSDKPIATVFLSSKGTPEVLSSGPR
                     GAIPSYSFPENAALALGAAARYGAWRQRPRGNQLRLEPDREQAIRTSIRAWTAGTAGP
                     AARWLSFSEISQLLTLAGVPVVDHRIAADPEAAVAAARELGFPVALKACVPRLIHKTD
                     VGGVALQLATPAAVTAAALSMRARFPTLEGYIVQRQVRPGVETLVGMTKDASLGPLLI
                     AGIGGVAVELYKDVAFRVTPVSDRDAADMLDHLRGRQLFDGFRGAPAADRPALIDVLQ
                     RISALIEIVPEMVELDLNPVICHAPGEGCIAVDGR"
BASE COUNT          248 a          583 c          763 g          210 t
ORIGIN      
        1 ccggccgtcg acggcgatgc agccctcgcc gggggcgtgg cagatcaccg ggttgaggtc
       61 gagctcgacc atctcgggga cgatctcgat cagggccgag atccgctgga ggacgtcgat
      121 cagcgcaggg cggtcggcgg ccggggcgcc gcggaagccg tcgaacagct ggcggccgcg
      181 caggtggtcg agcatgtcgg cggcgtcgcg atcgctgacc ggggtgacgc ggaacgcgac
      241 gtccttgtag agctcgacgg cgacgccgcc gatgccggcg atcaggagcg ggccgagcga
      301 ggcgtccttg gtcatgccga ccagggtctc gacgccgggg cggacctggc gctggacgat
      361 gtagccctcg agggtgggga accgggcgcg catcgagagc gccgcggcgg tgacggcggc
      421 gggggtcgcg agctggagcg cgacgccgcc gacgtcggtc ttgtggatca gccgggggac
      481 gcaggccttg agcgcgaccg ggaagccgag ctcgcgcgcg gcggcgacgg cggcctcggg
      541 gtcggcggcg atgcggtggt cgacgaccgg gacgccggcg agggtgagca gctgggagat
      601 ctcggagaac gacagccagc gcgcggccgg gccagcggtc ccggcggtcc acgcgcggat
      661 ggaggtgcgg atcgcctgct cgcgatcggg ctcgaggcgg agctggttgc cgcgcggccg
      721 ctgccgccac gcgccgtagc gagcggcggc gccgagcgcg agcgcggcgt tctcggggaa
      781 gctgtacgac gggatcgcac cgcgcgggcc cgacgacagg acctcggggg tgcccttgga
      841 cgacaggaac acggtggcga tcggcttgtc ggaggggagg gtgccggccc cgcgcgcgat
      901 cgcggcggcg accgcctcgg gctgggtgac cagcggcggg acgtagatcg cgacgacggc
      961 gtcgacgttg gggtcgcggc cgaccagctc gatcgcgcgc tcgtagtgct cgggcggcgc
     1021 cgaggcgatc atgtcgaccg ggttggcgac gctggcctgg gccggcagga agccgcgcag
     1081 ggcggcggcg gtggcctcgg cgaacggcga cagggtgagg ccgtgagcct cgcaggcgtc
     1141 ggcgagcagg atgcccgggc cgccggcgtt ggtgaccacg ccgatccgcc ggccggccgg
     1201 cacgggctgg ctcgacagca gggcgacgac gtcgaacatc tcctcgagcg tggtcgtgcg
     1261 gatcacgccg gtctgcgcga acagcgcgtc gatcccgacg tcgaggctgg cgagcgcggc
     1321 ggagtggctc gacgcggcgc gggtgccggc ggcggagcgc ccggacttga ccgcgacgat
     1381 cggccgctcg cgcgcgacct cgggggcgag gcgcgcgaac atccgcgggt tgccgaagct
     1441 ctcgaggtac agcgcgatca cccgggtgcg cggcgacgag tgccagtacg cgagcaggtc
     1501 gttgaccgag acgtcggcct tgttgccgat cgagacgaag tcggcgatgc cgaggtggag
     1561 ctccttggcg tagtcgagca tcgccaggcc gagcgcgccg ctctggctga gcatcgagac
     1621 gttgccggcg ggcgggaaga tcgggctgaa cgtggcgtcg agccggaccg ccggatcggt
     1681 cgacatcacg cccatgcagt tggggccgac caggcgcatg ccggcgccgc gcacgagctc
     1741 gcgcagccgc tgctcggcgg cgaggccgcc gacctcggcg aacccggcgg tgatcacgac
     1801 cacg
//
LOCUS       DASVOR010001590         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_162107,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001590 DASVOR010000000
VERSION     DASVOR010001590.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162107"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1676)
                     /locus_tag="VF516_29860"
     CDS             complement(<1..>1676)
                     /locus_tag="VF516_29860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX8111989.1"
                     /translation="SGGALTSSKFDLTLHLYDDPQGLRGLFEYATDLFDAATIERMAA
                     HWEVILEGIVEAPDRRVANISILTWSERQRLVEWNDTRDESPGTCLEDMFEAQAAKTP
                     EAVALVSGDEQLTYRELDERADALAAGLRRLGVGPEEAVAVCLERSPAWVVAMLGVLK
                     AGGIYVPIAVDDPPKRRALLVEEARPKVVLTHERFDALVASPAPHAERRVATPPESGA
                     WVIYTSGSTGRPKGVVVSHRSATHQLAVWQALHPVGPQDAILVSSAISFDLSILQVLW
                     PLLAGGRMVLIDEDGHRDPAVLAGAIRRHCIAKLVLVPSMLRMVLAEAEREALPSLKA
                     VFVGGEALPLDLAREVHRIVPQAELFNVYGPTEATILATAWPCPRDPPRILIGKPVPG
                     TSAYVLDAALEPVPYGVPGVLYLGGPAVARGYLGRPEVTAETFVPDPFSAGVPGARLY
                     RTGDTARRHPSGDIEFLGRADHQVKLRGYRIELGEIEAALVQYGNIGETIVLVRGAET
                     GHARLVAYVTPVTGTAPAPKQVQAYLRQRLPASMVPTAYVVMDRLPRMAS"
BASE COUNT          260 a          624 c          542 g          250 t
ORIGIN      
        1 gctggccatc cgaggcagtc gatccatcac gacgtaggca gtcgggacca tggacgcggg
       61 cagccgctgt ctcaggtagg cttggacctg cttcggcgcc ggcgctgttc ccgtcacggg
      121 cgtgacgtat gcgacgagcc gcgcgtggcc agtctccgcg ccccgcacga gcacgatggt
      181 ttcgccgatg ttgccgtact gcacgagcgc agcctcgatc tcgccgagct cgatgcggta
      241 gccgcgaagc ttcacctggt ggtccgcgcg cccgaggaac tcgatgtcgc cgctcgggtg
      301 ccggcgcgcg gtatcgcccg tccggtacag ccgtgcgcct gggacgccgg cggagaaagg
      361 atcgggcacg aacgtctcgg ccgtgacctc cggtcgaccc aggtagccgc gcgccaccgc
      421 gggtccaccc aggtagagga cgcccggcac accgtagggg acgggctcca gggcggcgtc
      481 caggacgtac gccgaggtcc cgggcacggg cttgccgatc aggatgcgcg gggggtcgcg
      541 cgggcatggc cacgcggtcg cgaggatcgt cgcttccgtc ggcccgtaca cgttgaatag
      601 ctctgcttgc ggaacgattc gatggacctc gcgtgcgaga tcgagcggca gcgcttcgcc
      661 gcccacgaac acggccttca acgacggcag cgcttcgcgc tcggcttcgg cgagcaccat
      721 gcgcaacatc gacgggacca agaccagctt ggcgatgcag tggcgccgga tcgcgccggc
      781 gagcaccgcc gggtcgcggt gaccgtcctc atcgatcagc accatcctgc cgcccgccag
      841 gagcggccag agcacctgca agatcgagag atcgaagctg atcgccgacg aaaccaggat
      901 cgcgtcctgc ggtcccaccg gatgcaacgc ttgccatacc gccagttgat gggtcgccga
      961 ccgatgactg acgaccacgc ccttgggcct tcccgtcgac ccggacgtgt agatcaccca
     1021 cgcgccgctc tccggaggcg ttgcaaccct gcgctccgcg tgcggcgccg gcgaggcgac
     1081 gagcgcgtcg aaccgctcgt gggtcagcac gaccttcggt cgcgcctctt cgaccaagag
     1141 agcccggcgc ttcggcggat cgtcgaccgc gatcggcacg tagatcccgc ccgccttcag
     1201 caccccgagc atcgcgacca cccaggccgg cgaccgctcc aggcacaccg ccaccgcttc
     1261 ctccggaccc acgcccaacc gacgcagccc cgccgcgagc gcgtccgccc gctcatcgag
     1321 ctcccggtac gtcagctgct cgtctcccga caccaacgcg acggcctccg gcgtcttcgc
     1381 cgcctgcgcc tcgaacatgt cctccaggca cgtgcctggc gattcgtcac gtgtgtcgtt
     1441 ccattcgacg agtcgctgcc gctcggacca cgtcaggatc gagatgtttg ccacgcgtcg
     1501 gtcgggcgcc tccacgatcc cctcgaggat cacctcccag tgcgctgcca tgcgctcgat
     1561 cgtcgccgcg tcgaacaggt ccgtcgcata ctcgaacaac ccccgcaggc cctgcgggtc
     1621 gtcatagagg tgcagcgtca ggtcgaactt cgagctggtc agcgccccgc cgctgc
//
LOCUS       DASVOR010001591        14229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_424682,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001591 DASVOR010000000
VERSION     DASVOR010001591.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14229
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424682"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1563)
                     /locus_tag="VF516_29865"
     CDS             complement(<1..1563)
                     /locus_tag="VF516_29865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111990.1"
                     /translation="MAELQPSEVLFIPKRKRLTHGRFKNEDGQDVLHLFYGEVELIFD
                     EPDIAPVGEKLIEVERFRAEEAMAWSNAGPHAWERIRDLLQALIDQNVLTRASDVPAA
                     GKENFPLRLGLAPEGREPRAFSAADEQRCPVLTEEAFGRAFDLSNLEVVVPIYRVAHP
                     AMDTDGRQVGENNVMPRTLFLDLPTQRKLCGFAGSRYQDDLPMNVTAMKQMAKRWPEL
                     LSLSEQYREALWARMPPRDPSSLRTGEVHLLAVGQLAAVAYVMVRGWDPVPNGQLDGG
                     LAAMFRLIDGVRLVTNDIMRASPTAHGCETPLNAWGINDWAERHAVYRGTWGVCAGPP
                     GLIDEYLRVTLAETSAPIRVEPGLAERLGDLEAAIDYGLLGQRLESAVRFFGVSQGLL
                     HERLRAAFEGQSPRTQLHDRVDVAVNMQNFPLLREDHALADTFRLEADMDRWLFARAA
                     EGLPGKVDGASLDELMQLDPAAQAASQRRLAEFFAHALPADKAVSEPICSELAAVAAD
                     GFALERRCLRVVA"
     gene            complement(1582..3072)
                     /locus_tag="VF516_29870"
     CDS             complement(1582..3072)
                     /locus_tag="VF516_29870"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8111991.1"
                     /translation="MTRTIAVLVTTSLAGACALVRPVRLNPPPPPAAFVVDLAAPPST
                     APGATDAPGGDERLTHNPVWWSAFADPALDAVIQEALRNNYFIRDVRTLITEDMLDST
                     VPRGWLWPLQVGILPTTPAQLQHVVAASPPGVAVDTNGVPQKIAAAKNAYNAANIDLT
                     ASYQLDVWGNLDAQRRVADDLVEQQRQSTELASQNTAEQVTQLWFDILVQRALKELTE
                     GEVKYNQELFNLVKARFEQHLTPRLVVLQQEQQLLNIQAQVPLIATRIALLNSQLTAL
                     LGRLPNPQDEMVPADRRLPDLPPEPRIGTPGDLIENTVEMRFAKLRVTEIENRINQNL
                     SSWLPVIDLVASTGVVTFGSSKETFQESFAGIRLIWPIFDGGRRLIDAKQLQLTLKRR
                     KWQYDLAIKTAVGRVQDALLQEKYQAESLRSLRGQIELGRRVLDEARRLFEQGQSDYL
                     PVLNALSSLAALERSGLQAQRQLLSYRVQLYRALGGTWSYDVTKLP"
     gene            complement(3080..5203)
                     /locus_tag="VF516_29875"
     CDS             complement(3080..5203)
                     /locus_tag="VF516_29875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8111992.1"
                     /translation="MTPDVIQQGHLLWPVDRLPDALEQIALRAGYGVRPGELGAHDPA
                     VEPSRIWMFALAGRLGVELEPVTPRYRELPAFVARSAPAVLQIRRNGRPFYLVLLGRR
                     WNKVRLLTRDGAVVSVDASGVLALVRQEKQARVAAEVDQLLAGVETSGRARERVRAKL
                     LLQRLGQEELRTCWIMRPQRTARARTLLGDIPTLVAGILVNHMLLSLVLAASFWILGR
                     AALQAHLETGWFEGWIAVIACAVPLRLLEVWWQGLFSIRIGTLLKQQLLTGILKLTPD
                     EIRLDGIGKHFGRVAESEVVELLTLGGALLAVLSLVDLILAAIILALGAGGWLHVAVL
                     AAWVLVAFALAARHYKAQRRWSLARIDITHDLLERMLGHRTRLAQLPLERWHDGEDRR
                     LADYCETSQAMDRRTMQLSALLRDGWLVLALVTLLPAFAGGNTPSSALAVSAGGILLA
                     LRAFDEISVFFQQISLAAASFEQIRDLLRAVVRPELESKVPLEMEAGKLTEEVGGTLI
                     EARGLTFRHHARTRPVLENCSLQIARGDRILLEGPSGGGKSTLVSLLTGLRTPEAGVL
                     LLRGLDRSTFGMSGWRRRVTAAPQFHENHVLNGSFAYNLLLGREWPTSGEMRDKAQAL
                     CEELGLGELVSRMPSGLDELIGETGWQLSHGEKSRLYIARTLLQGVELVILDESFASL
                     DPETMRVAVRCVLEHAPSVVVVSHP"
     gene            complement(5200..7935)
                     /locus_tag="VF516_29880"
     CDS             complement(5200..7935)
                     /locus_tag="VF516_29880"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8111993.1"
                     /translation="MTPRPRPRTIIPEVIQISATDCGPASIKSVFAGMGLELNYRLLR
                     EACQTDVDGTSIDTLEELANQLGLDAEQIMIPMDHVFLPEAHALPAIIVTMTAGGRQH
                     FVVLWKRFGPFVEVMDPVVGRRWAHIETLASTFYQHTTSAPAAAWREWAGSQEAVAAL
                     ERRLLNLGAAGAKALIAKALEDPGYRSIATLDAAARAVEAMVRAGALGRGRTAAGLVQ
                     SMIAKAESGEVPIPEAYWSVRPNPDAEDQLLLTGAVLLRFRGWRADATGSEAAAEAPR
                     SANPELATALAAPQSSTAQTLFRLLREDPRSIPLLITAGLVISTFGRIVQALMLRAVL
                     DLGRDLGTVGQRAAGILVLAGIAVALLTIQVPITLGLLGIGRRLEVRLRKAYFEKLPK
                     TEDRYFQSRLASDMASRCHNIQAMRQLPNLIAQGITVSLEVLATAAALIWAAPGAWWI
                     ALALTAATLLLPLLAQRMFFEPDMRVQTHAGSLSSFILNALLGLTPIRIHGAERSLRR
                     GQEAMLVEWTKSRYWLQRLSVGFEGMLMVASYALVVLLVYSYLQTSMRASLVLLVVFW
                     ALRFPILGQRLLVLTRAFPSAMNRVRRLLDIIGEIQPEPGRSPEPALEPELRAIPASP
                     AGEAPDHEPVAPAAAAASGVAISMERVRIKGGGHTILDKVSLNIAAGEHVAVVGVSGA
                     GKSTLVGLLLGWLRPARGEIRVDGELLDQPAIERLRRTTAWVDPAISIWNQTLLDNLR
                     YGNDSAHGWALDAVLRGAEMLDILEALPAGLQTSLGEGGGLVSGGQGQRVRLARAMLR
                     SGVRLAILDEPFRGLDRDRRARLLAESRRLWANITLLCVTHDVAQTQEFDRVLVVENG
                     RIIENGPPKQLLAKDKSRYAELLRADLENHQLIWSGGKWRHWWLADGKLVEKSAS"
     gene            complement(7942..9180)
                     /locus_tag="VF516_29885"
     CDS             complement(7942..9180)
                     /locus_tag="VF516_29885"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX8111994.1"
                     /translation="MAYPFERTLRSLNGYESGTRVLLVALVTLGLGGLAAWAIFARVP
                     VMKVSSQGRIEPHNAVYRLEPPAAGRVVRSMLNLDEEVKQGDLLIEFDTRSEQLELEQ
                     SKTSSVAQEQELAVIRDQITNKKDELAASGLVDEVAVREAEEREKELAPRRRVAEQRA
                     QLAQQSPSGSISELEKLERVTEADSLKSTSRAQGLEVVRLRREQAVRRQTLAAQLLGL
                     RREELRLEGSLRELKVTIDKLQYQIEKKRYRAPASGRLVDVVELAPGDFIADGQRMGT
                     ILASDAEVRVRARFPKESVGIIQPGQTAQLKLDGYPWTIYGTVPARVTRVGTEPGIAA
                     TPEAIPGTVRVELDIQPSLDPRIQLQHGLTATVEVEVARVSPLALLMRAIGEWDVTQT
                     APAAPEERRARGFQAETEAR"
     gene            9418..9678
                     /locus_tag="VF516_29890"
     CDS             9418..9678
                     /locus_tag="VF516_29890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111995.1"
                     /translation="MGDVEEGAIPQGWFDSVRSETLATRRHRALPLDDAPREVQPAKQ
                     LGFDSVVRIDAGGNVCHAMCNHRPRAPIANDNPFYESPRSWI"
     gene            10080..12155
                     /locus_tag="VF516_29895"
     CDS             10080..12155
                     /locus_tag="VF516_29895"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8111996.1"
                     /translation="MALPVVLAVSCARPSPGPAKLAETSTDPGPAPAAAPASATTQRG
                     IEAGDVNRSVDPCSDFYEYANGTWRTHNPIPAGKPRWSRRDIGRETNRQNVRELVQEL
                     AAKTDWSAGSPEQLVGDHYASCMDEAAIDAAGISPIAPLLADIDAAKTPADVQRVIRR
                     LHDIGVTAPFGETAAFDNSEPKDFLLNLVAGGLGLPDRDAYLKTEPRFAEIRDKYRQH
                     VARVLALGGMPQNATLAAADGVIALEKRLAEASLDAKTAADPAATEHKTTFAQLKAMA
                     PHVDWERYFDEARIPREPVNVTEPKFLQQVDKELKTTPVAAWKTYLRWQLLDTASPWL
                     SRAFAEEAFAFKDKTLGGATEMRPRAQRCLELTETLLGEPVGRKYADKYFPPAAKAKA
                     REIADGLLAALKEDVSGLPWMAAETKKKALEKLELMSVQLGYPDHWKDLSNVQIRRDT
                     LWANIAQARKFNVDDLRRQVGKPTDRNGWQLPASSPFAYLDAQLNELVLPAGFLQPPY
                     FDVAATDARNYGALGSGLAHDMTHAFDATGALLDAMGHAKTWWTDADQREFQKRAQCI
                     MDQYESYSVEPGVHHQGKLVLNEALGDQAGVHFAYLALKKSMITHPVPTVDGFTAEQQ
                     FFLAWAQFRGEAVRIEAQRQIVKDDPHAVPKFRVIGPLSNLPEFQQAFACKAGAPMVR
                     SGEQRCAVW"
     gene            complement(12603..13568)
                     /locus_tag="VF516_29900"
     CDS             complement(12603..13568)
                     /locus_tag="VF516_29900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8111997.1"
                     /translation="MARVLKIFFHDACFDGTTSAALFAAFYRDVLDRGVRVLPVGMVH
                     RDGNPFEGVPLDADDHACVDFRFCADSRMRWWFDHHPTAFQPPALREVFEREHRPTWF
                     FDPAALSCAGLIARELEAGWRWKPPPHLVEATRWADTIDAARFASAEDAVALTLPAQR
                     IAAWLAHGRTPVDTAHYVEWLSRESLAQITARPEVAVALAVVESERARDLEEIRRLGV
                     WHGDVIVFDRFADPGARSPGFLGYLLYPSCLYAVSGTQHSQNIKISVGVNPWSKQRRR
                     HDIGALCARFGGGGHAAVGGVTLEPDEIGRARATMAAIVHELATT"
     gene            13638..14090
                     /locus_tag="VF516_29905"
     CDS             13638..14090
                     /locus_tag="VF516_29905"
                     /inference="COORDINATES: protein motif:HMM:NF015608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEX8111998.1"
                     /translation="MHQWLLSAHLIGVIFWLGCLFAVYWLLRVHAQAPRNVLDKLTLM
                     ERSLALTMDLAAALAIGTGLAMALRHDIDPVHTLFTKPGAGWFHIKLTVVVLGILPVH
                     GMVRGKIAKFSRGVISNVPQWIWSLLLISIVIIVVMVIRGPLMFASST"
BASE COUNT         2236 a         4934 c         4922 g         2137 t
ORIGIN      
        1 cgcgacgact cgcaggcagc ggcgctcgag cgcgaacccg tcggccgcga cggctgccag
       61 ctcgctgcaa atcggctcgc tcaccgcctt gtcggccggg agtgcgtgcg cgaagaactc
      121 ggcgagccgg cgctggctcg ccgcctgcgc ggccgggtcg agctgcatga gctcgtcgag
      181 cgaggcgccg tcgaccttcc ccggcagccc ctcggcggcg cgcgcgaaca gccagcgatc
      241 catgtcggct tccagcctga acgtgtccgc cagcgcgtgg tcctcgcgga gcagcgggaa
      301 gttctgcatg ttgaccgcca catccacccg gtcgtggagc tgggtgcgcg ggctttggcc
      361 ctcgaatgcc gccctcagcc gctcgtggag caggccctgc gagaccccga agaaccggac
      421 cgccgactcg agccgctggc cgagcaggcc gtagtcgatc gcggcctcga gatcgccgag
      481 ccgctccgcc aggcccggct cgacccggat cggcgcgctg gtctcggcga gtgtcacgcg
      541 gagatactcg tcgatgagcc cagggggccc ggcgcacacg ccccacgtgc cgcgatacac
      601 cgcgtgacgc tcggcccagt cgttgatgcc ccaggcgttg agcggcgtct cgcagccgtg
      661 cgcggtgggc gaggcgcgca tgatgtcgtt ggtgaccagc cggacgccgt cgatcagccg
      721 gaacatcgcg gccagcccgc cgtcgagctg cccgttgggc accgggtccc agccccgcac
      781 catgacgtag gcgaccgcgg ccagctggcc caccgccagc aggtggactt ctccggtccg
      841 gagcgacgac ggatcgcgcg gcggcatgcg cgcccacagc gcctcccggt actgctccga
      901 cagcgacagc agctccggcc accgcttggc catctgcttc atcgcggtga cgttcatcgg
      961 gaggtcgtcc tggtagcggc tccccgcgaa gccgcagagc ttgcgctgcg tcggcagatc
     1021 gaggaacaac gtgcgcggca tgacgttgtt ctcgccgacc tgccggccgt cggtgtccat
     1081 cgcggggtgg gcgacccggt agatcgggac gaccacctcg aggttggaca ggtcgaacgc
     1141 ccgcccgaag gcctcctcgg tgaggaccgg gcagcgctgc tcgtcggcgg cgctgaacgc
     1201 gcgcggctcg cggccctcgg gcgccaggcc gagccgcagg gggaagttct ccttgccggc
     1261 cgcgggcacg tcggaggccc gggtcagcac gttctgatcg atcagcgcct ggagcaggtc
     1321 gcggatccgc tcccaggcat ggggccctgc gttcgaccac gccatggcct cctccgcgcg
     1381 aaaccgctcg acctcgatca gcttctcgcc caccggcgcg atgtcgggct cgtcgaagat
     1441 cagctcgacc tcgccgtaga acaggtgcag gacgtcctgg ccgtcctcgt tcttgaaccg
     1501 gccgtgcgtc agccgcttcc gcttgggaat gaagagtacc tcgctcggtt gaagttccgc
     1561 catggcgtgt cctgccttca gtcagggaag cttggtgaca tcataggacc aggtgccgcc
     1621 cagagcacgg tagagctgga ctcgatagga tagcagctgc cgctgcgcct ggaggccact
     1681 gcgttcgagc gcggccaggc tggagagcgc attgagcacg gggagatagt ccgattgccc
     1741 ctgctcgaac agtcgccgcg cttcatccag cacgcgccgg ccgagctcga tctgcccgcg
     1801 caggctgcgc aggctctcgg cctggtactt ctcctgcagc agcgcgtcct gcacgcggcc
     1861 gaccgccgtc ttgatggcga gatcgtattg ccacttccgc cgcttgagcg tcagctggag
     1921 ctgcttggcg tcgatcaggc gccggccgcc gtcgaagatc ggccagatca ggcggatccc
     1981 cgcgaacgac tcctggaacg tctccttcga ggacccgaac gtgaccacgc ccgtgctggc
     2041 caccagatcg atcaccggca gccagctcga caggttctgg ttgatgcggt tctcgatctc
     2101 ggtcacgcgc agcttggcga accgcatctc gaccgtgttc tcgatcaggt cgcccggcgt
     2161 cccgatccgg ggctccgggg gcaggtccgg caggcggcgg tccgccggaa ccatctcgtc
     2221 ctgggggttc ggcagccggc cgagcagcgc ggtcagctgc gagttcagga gcgcgatccg
     2281 cgtggcgatc agcgggacct gcgcctggat gttgagcagc tgctgctcct gctggaggac
     2341 gaccagccgg ggcgtgaggt gctgctcgaa ccgcgccttg accaggttga acagctcctg
     2401 gttgtacttg acctcgccct cggtcagctc cttcagggcg cgctgcacca ggatatcgaa
     2461 ccacagctgg gtcacctgct cggcggtgtt ctgggacgcg agctcggtgc tctggcgctg
     2521 ctgctcgacc agatcgtcgg cgacccggcg ctgcgcgtcg aggttgcccc acacgtcgag
     2581 ctggtaggac gcggtgaggt cgatgttcgc cgcgttgtac gcattcttcg ccgcggcgat
     2641 cttctgcgga accccgttgg tatccacggc cacgcccggc ggcgaggcgg cgacgacgtg
     2701 ctggagttgc gccggcgtcg tgggcaggat gccgacctgc agcggccaca gccagccgcg
     2761 cggcaccgtc gagtcgagca tgtcctcggt gatcaacgtc cgcacgtcgc ggatgaagta
     2821 gttgttgcgg agcgcctcct ggatcacggc gtcgagcgcg gggtcggcga acgcgctcca
     2881 ccacaccggg ttgtgggtga gtcgctcgtc cccgcctggc gcgtcggtcg cgcccggggc
     2941 ggtggacggg ggggccgcga gatccacgac gaaggccgcg ggcggcggcg gtggattcaa
     3001 ccggaccggc cggaccagcg cgcacgcgcc ggcgagcgag gtcgtcacga gcactgcgat
     3061 cgtgcgggtc atgggcggct catgggtggc tcacgaccac cacggatggc gcgtgctcga
     3121 gcacgcagcg caccgccacc cgcatggtct cgggatccag cgacgcgaag ctctcgtcga
     3181 ggatgaccag ctcgacgccc tgcagcagcg tgcgcgcgat gtacagccgg ctcttctcgc
     3241 cgtgcgagag ctgccagccg gtctcgccga tcagctcgtc cagcccggac ggcatccggg
     3301 acaccagctc gccgagcccg agctcctcgc acagcgcctg ggccttgtcc cgcatctcgc
     3361 cggaggtcgg ccactcgcgc ccgagcagca ggttgtacgc gaagctgccg ttgaggacat
     3421 ggttctcgtg gaactgaggc gcggcggtga cgcgccgccg ccagcccgac attccgaagg
     3481 tggaacggtc gaggccgcgc agcagcagca cgcccgcttc cggcgtccgc aggccggtca
     3541 acagcgagac cagcgtcgac ttgccgccgc ccgacggccc ctcgagcagg atgcgatcgc
     3601 cgcgcgcgat ctgcagcgag cagttctcca gcacgggccg ggtccgcgcg tggtgccgga
     3661 aggtcaagcc gcgcgcctcg atcaaggtcc cgccgacctc ctcggtcagc ttgccggcct
     3721 ccatctcgag cgggaccttc gactcgagct ccggccggac caccgcgcgc agcagatccc
     3781 ggatctgctc gaacgacgcc gcggccagcg agatctgctg gaagaacacg gagatctcgt
     3841 cgaaggcgcg cagcgcgagg aggatgccgc cggcggacac cgccagcgcg ctggatggcg
     3901 tgttgccgcc ggcgaatgcc gggagcagcg tcaccagcgc gaggaccagc cagccgtccc
     3961 ggagcagggc cgacagctgc atggtccgcc ggtccatggc ctgcgaggtc tcgcagtagt
     4021 cggccaggcg gcggtcctct ccgtcgtgcc agcgctcgag cgggagctgg gcgagccgcg
     4081 tccggtggcc gagcatgcgc tcgagcaggt cgtgcgtgat gtcgatgcgc gccagcgacc
     4141 accggcgctg ggccttgtag tgccgcgccg cgagcgcgaa cgcgaccagc acccaggcgg
     4201 cgagcacggc gacgtgcagc cagccgcccg cgccgagcgc gaggatgatc gccgccagga
     4261 tcagatcgac gagcgagagc accgcgagca gcgcgccgcc gagcgtgagc agctcgacca
     4321 cctccgactc cgcgacgcgc ccgaagtgct tcccgatgcc gtcgaggcgg atctcgtccg
     4381 gggtcagctt gaggatgccg gtcaggagct gctgcttgag cagcgtgccg atccggatcg
     4441 agaacaaccc ctgccaccac acctcgagca ggcgcagcgg gaccgcgcag gcgatgacgg
     4501 cgatccagcc ctcgaaccag ccggtctcga ggtgggcctg gagcgcggcg cgcccgagga
     4561 tccagaacga cgccgccagc accagcgaga gcagcatgtg gttgacgagg atgccggcca
     4621 ccagcgtcgg gatgtcgccg agcagcgtcc gggcgcgcgc ggtgcgctgg gggcgcatga
     4681 tccagcaggt ccgcagctcc tcctgaccga gccgctggag cagcagcttg gcccggacgc
     4741 gctcgcgcgc gcgccccgag gtctcgacgc ctgcgagcag ctggtcgacc tcggcggcga
     4801 cccgggcctg cttctcctgg cgcaccagcg cgagcacgcc gctcgcgtcg accgagacca
     4861 ccgccccatc ccgcgtgagc agccggacct tgttccagcg cctgccgagc agcaccagat
     4921 agaacggccg gccgttgcgc cggatctgga gcaccgcggg cgcggagcgc gcgacgaagg
     4981 ccgggagctc gcggtagcgc ggcgtgaccg gctcgagctc gacgcccagc cggccggcca
     5041 gcgcaaacat ccagatccgg ctcggctcga ccgccggatc gtgcgcaccg agctctccgg
     5101 ggcgcacgcc gtagcccgcc cggagcgcga tctgctcgag ggcgtcgggc aaccggtcga
     5161 cgggccacag caggtgcccc tgctggatga cgtcgggtgt catgacgccg acttctccac
     5221 gagcttgccg tccgcgagcc accagtgccg ccactttcca ccgctccaga tcagctggtg
     5281 gttctcgagg tccgcgcgga gcagctccgc gtagcgcgac ttgtccttgg ccaggagctg
     5341 cttcggcggg ccgttctcga tgatccgccc gttctcgacc accaggaccc ggtcgaactc
     5401 ctgggtctgc gcgacgtcgt gggtcacgca caagagcgtg atgttcgccc acagccgccg
     5461 ggactccgcg agcaggcgcg cgcggcggtc gcgatcgagc ccgcggaacg gttcgtcgag
     5521 gatcgccagc cgcacaccgg agcggagcat cgctcgggcc aggcgcacgc gctggccctg
     5581 cccgccggag accagccctc cgccctcgcc gagcgaggtc tgcaagcccg ccggaagcgc
     5641 ctcgaggatg tcgagcatct cggcgccgcg cagcaccgcg tcgagcgccc agccgtgcgc
     5701 ggagtcgttg ccgtacctca ggttatcgag cagggtctgg ttccagatgc tgatcgccgg
     5761 atcgacccag gcggtggtcc ggcgcagacg ctcgatcgcg ggctgatcga gcagctcgcc
     5821 gtcgacgcgg atctcgccgc gcgcgggacg cagccagccg agcaggaggc cgaccagcgt
     5881 cgacttgccg gcgcccgaca cgccgacgac cgccacgtgc tcgccggcgg cgatgttcag
     5941 cgagaccttg tcgaggatgg tatggccgcc gcccttgatg cggacacgct ccatcgagat
     6001 cgcgacgccg ctcgccgcgg ccgcggcggg cgccaccggc tcgtggtccg gagcctcgcc
     6061 ggcagggctg gcagggatcg cgcgcagctc cggctcgagc gcgggctccg ggctccggcc
     6121 gggctccggc tggatctctc cgatgatgtc cagcaggcgg cggactcgat tcatcgcgct
     6181 cgggaacgcg cgggtcagga ccagcagccg ctggccgagg atcgggaacc gcagcgccca
     6241 gaacacgacc agcaggacca gcgaggcgcg catgctggtc tggaggtacg agtagacgag
     6301 cagcacgacg agggcgtagc tcgccaccat cagcatgcct tcgaacccga cggacagccg
     6361 ctggagccag tagcgggact tggtccactc gacgagcatc gcctcctgcc cgcggcggag
     6421 cgatcgctcc gcgccgtgga tccggatcgg cgtcaggccg aggagcgcgt tcaggatgaa
     6481 gctgctcagc gagccggcgt gggtctgcac ccgcatgtcg ggctcgaaga acatgcgctg
     6541 cgcgagcagc ggcaagagca gcgtggccgc ggtgagggcg agcgcgatcc accacgcgcc
     6601 cggggccgcc cagatcagcg cggccgccgt ggccagcacc tccagggaga ccgtgatgcc
     6661 ctgggcgatc aggttgggca gctgccgcat cgcctggatg ttgtggcagc gcgacgccat
     6721 gtcggaggcg agccggctct ggaagtagcg atcctcggtc ttgggcagct tctcgaagta
     6781 ggccttccgg agccgcacct cgagccggcg gccgatgccc agcaagccga gcgtgatcgg
     6841 gacctggatc gtgagcagcg ccaccgcgat gcccgccagc acgaggatgc ccgcggcgcg
     6901 ctgcccgacg gttccgagat cgcgcccgag atcgagcacg gcgcgcagca tcagcgcctg
     6961 gacgatccgg ccgaaggtcg agatcacgag cccggccgtg atcaacagcg ggatgctccg
     7021 cggatcctcc cggagcaggc ggaacagcgt ctgcgcggtg ctggactggg gcgctgcgag
     7081 cgccgtcgcg agctcggggt tcgcggaccg cggggcctcg gcggcggctt cgctgccggt
     7141 cgcgtccgcg cgccagccgc ggaaccgcag caacaccgcg ccggtcagca ggagctggtc
     7201 ttccgcgtcg gggttcgggc ggaccgacca gtaggcctcg gggatcggca cctcgccgga
     7261 ctcggccttg gcgatcatcg actgcaccag gccggccgcc gtgcgtccgc gcccgagcgc
     7321 gcctgcgcgc accatggcct cgaccgcgcg ggccgcggcg tcgagcgtgg cgatcgagcg
     7381 atagcccggg tcctcgagcg ccttcgcgat cagcgccttc gctccggcgg cgcccaggtt
     7441 gagcaggcgg cgctcgagcg cggccaccgc ttcctgcgag ccggcccatt cccgccaggc
     7501 ggccgcgggc gcggacgtcg tgtgctggta gaacgtgctg gccagcgtct cgatgtgggc
     7561 ccaccggcgg ccgaccaccg gatccatcac ctccacgaac ggcccgaagc gcttccacag
     7621 gaccacgaag tgctgccgcc cgccggccgt catcgtgacg atgatcgccg ggagcgcgtg
     7681 cgcttccggg aggaagacgt ggtccatcgg gatcatgatc tgctcggcgt cgagaccgag
     7741 ctggttcgcg agctcctcca gcgtgtcgat cgaggtaccg tcgacgtcgg tctgacacgc
     7801 ctcgcgcagc agccgatagt tgagctccag gcccatcccg gcgaacacgc tcttgatcga
     7861 ggccggtccg cagtcggtcg cggagatctg gattacctcg ggaatgatcg tgcgcgggcg
     7921 cggacgaggc gtcatgacgt ctcagcgagc ctcggtctcg gcctggaacc cgcgggcgcg
     7981 ccgctcttcg ggagccgcgg gcgccgtttg cgtgacgtcc cattccccga tggcacgcat
     8041 caggagcgcg agcggcgaga cgcgggcgac ctcgacctcg accgtggccg tcagcccgtg
     8101 ctggagctgg atccgcgggt cgagcgacgg ctggatgtcg agctcgacgc ggaccgtgcc
     8161 cgggatggcc tccggcgtgg cggcgattcc cggctccgtc ccgacgcggg tgacccgggc
     8221 cggcaccgtc ccgtagatgg tccagggata gccgtcgagc ttgagctgcg cggtttgacc
     8281 gggctggatg atgccgacgc tctccttggg gaaccgcgcg cgaacgcgga cctccgcatc
     8341 gctcgcgagg atggtgccca tccgctggcc gtccgcgatg aagtcgcccg gtgcgagctc
     8401 gaccacgtcg accagccgcc ccgaggcagg cgcgcggtag cgcttcttct cgatctgata
     8461 ctggagcttg tcgattgtca ccttgagctc tcgcagcgag ccctcgagcc ggagctcctc
     8521 gcgcctcagc ccgaggagct gggcggcgag cgtctggcgc cgcacggcct gctcgcgccg
     8581 gagccggacg acctccaggc cctgggcgcg gctggtggac ttgagcgaat cggcctcggt
     8641 gacacgctcg agcttctcga gctcggagat cgagccggac gggctctgct gggcgagctg
     8701 cgcgcgctgc tcggccaccc ggcgccgcgg cgcgagctcc ttctcccgct cctcggcctc
     8761 gcggactgcc acctcgtcga ccaggccgct cgcggcgagc tcgtctttct tgttcgtgat
     8821 ctgatcgcgg atgaccgcga gctcctgctc ctgtgccacc gagctggtct tgctctgctc
     8881 gagctcgagc tgctcgctcc gcgtatcgaa ctcgatgagc agatcgcctt gcttgacctc
     8941 ttcgtcgagg ttgagcatcg agcgcaccac tcgacccgcc gcgggcggct cgagccggta
     9001 gaccgcgttg tgcggttcga tgcggccctg cgagctgacc ttcatcacgg ggacgcgggc
     9061 gaagatggcc caggcggcga gccctccgag acccagtgtg accagggcga ccagcaggac
     9121 ccgggtgccc gactcgtatc cgttcaagga ccggagcgtc cgctcgaagg gatatgccat
     9181 tgcgtgatca gagagtagac cggaagcgcg tcagctgcac ggtcaatatt cgaccgattc
     9241 gagatggagt agctgggacc ggatccaaag atccggtccc agcgctatcg cggaattagc
     9301 gcgccttcag gacgctgcca gcgcgaacgt ggactttgat cttcattgat tcaccccctt
     9361 tcttcttctt cgttataatc aagaaggcag cacaccgcaa acgagaaatg agaatttttg
     9421 ggcgacgttg aagagggcgc gattccgcag ggttggtttg actcggtgcg tagcgagacg
     9481 ctggccacgc gccggcatcg tgcgctcccg ctcgacgacg cgccgcgtga agtgcagccc
     9541 gcaaagcagc ttggcttcga ctcggtggtc cggattgacg ccggcggtaa cgtttgccat
     9601 gcgatgtgca accatcgtcc acgcgcgccg atcgccaatg acaacccgtt ctatgagtct
     9661 ccgcgcagct ggatctgaca agccgcgagg gctgaataca cctctgttgt aagccactcg
     9721 cggcgctcgc ggttgtcgac gacgatcgag gggatctcgt agcggtggct ggacccttcg
     9781 ccggaaagca ggggaatcct gtattgcatc aagacccgag ttgtgactct ccgccggtga
     9841 acgcgaagtg gtccaggtgg tgagtcagtc cgcgccgtat tcgtcgctgt cgctccgttc
     9901 ggcctgacct gcttcgccgg cggaaacgcc ggacccgcga ttgatcggtt cctgcgatcc
     9961 tgcgaacctc cgcgtgctcg cgccgatgcc cggcgcgtcg cagctggaag gaacagccga
    10021 tgcgaaccgt cttgtcgatc cgggaggcac tcatgaaacc gcatgtcatc gcgagcgccg
    10081 tggctcttcc ggtggtgctc gccgtgtcgt gcgcgaggcc gtcgcccggg cccgccaagc
    10141 tggcggaaac atcgaccgat cccggccccg cgccggccgc cgctccggcg agcgcgacga
    10201 cgcagcgagg gatcgaggcg ggcgacgtca atcgcagcgt cgatccgtgc agcgacttct
    10261 acgagtacgc caacggcacg tggcgcacgc acaacccgat ccctgcgggg aaaccgcggt
    10321 ggagccgccg cgacatcggg cgcgaaacga accgccagaa cgtgcgcgag ctcgtccagg
    10381 agctcgcggc gaagaccgac tggtcggcag ggagccccga gcagctggtc ggcgatcact
    10441 acgcgtcttg catggacgag gcggcgatcg acgcggcggg catctcgccg atcgcgccgc
    10501 tgctcgccga catcgatgcg gcgaagacgc cggccgatgt gcagcgcgtc atccgccggc
    10561 tgcacgatat cggcgtcacc gcgccgttcg gcgagaccgc cgcattcgac aactccgagc
    10621 cgaaggactt cctgctcaac ctcgtcgcgg gcggcctcgg gctccccgat cgcgatgcgt
    10681 acttgaagac cgagccgcgg tttgccgaga tccgcgacaa gtaccggcag cacgtggcgc
    10741 gcgtgctcgc tctcggggga atgccgcaga atgcgacact cgccgccgcc gacggcgtga
    10801 tcgcgctcga gaagcgcctg gccgaggcat cgctcgacgc gaagaccgcc gccgatcccg
    10861 cggccaccga gcacaagacg acgttcgcgc agctcaaggc gatggcgccc cacgtcgact
    10921 gggagcggta cttcgacgag gcgcggattc cgcgcgagcc ggtcaacgtc accgagccga
    10981 agttcctcca gcaggtcgac aaggagctca agaccacgcc ggtcgccgcg tggaagacgt
    11041 acctccgttg gcagctgctc gacacggcgt cgccgtggct gtccagggcg ttcgccgagg
    11101 aagcgttcgc gttcaaggac aagacgctcg gtggcgccac cgagatgagg ccgcgcgcgc
    11161 agcgctgcct cgagctgacc gagacgctgc tcggcgagcc cgtcggcagg aaatacgccg
    11221 acaagtactt ccccccggcc gcgaaggcaa aggcgcgcga gatcgcggac ggcctgctcg
    11281 cggccctgaa ggaggacgtc tcgggactgc cttggatggc cgcggaaacc aagaagaaag
    11341 cgctcgagaa actcgagttg atgagcgttc aactggggta tcccgaccac tggaaggacc
    11401 tgtcgaacgt gcagatccgg cgagatacgt tgtgggcgaa catcgcgcag gcgcggaagt
    11461 tcaatgtcga cgacctgcga cgccaggtcg gaaagccgac ggatcgcaac gggtggcagc
    11521 tcccggcgtc gtcccccttc gcatacctcg atgcgcagct caacgagctc gtcctccccg
    11581 ccgggttctt gcagccgccg tacttcgacg tcgcggcgac cgacgccagg aactacggtg
    11641 cgctcggatc gggcctggcg cacgacatga cgcacgcctt cgacgcgacc ggagcgctgc
    11701 ttgacgcaat gggtcacgcg aaaacctggt ggactgacgc ggatcagaga gagttccaga
    11761 agcgcgcgca gtgcatcatg gaccagtacg agagctattc ggtcgagcca ggcgtccacc
    11821 accagggcaa gctggtgctc aacgaggcgc tcggcgatca ggccggcgtg cacttcgcgt
    11881 acctcgcact caagaagtcc atgataacgc atccggtgcc gacggtcgac ggcttcacag
    11941 cggagcagca gttcttcctc gcgtgggccc agttccgtgg cgaagcggtg cggatcgagg
    12001 cgcagcggca gatcgtcaaa gacgacccgc acgcggtgcc aaagttccgg gtgatcggcc
    12061 cgctgtccaa cttgccggaa ttccagcagg cgttcgcctg caaggccggc gcgccgatgg
    12121 tgcgatcggg cgagcagcgc tgcgccgtgt ggtagtgcgc gcccggctgg tcggcaggtt
    12181 ccgacagacg taacaaaaca gacgtaacaa ataagaaagg cagggattca gatcgcgaga
    12241 tctgatccct gccttttgcg caatcagcgc gctcagacgc gcagcttgct gccagcgcga
    12301 acgtggacct tgatcttcat tgagtcaccc cctttcttgt ctctttataa agcacgaagg
    12361 attggcactg caaaacaaga atcgagtttt ttttggtgat caaagaagac gacacgattc
    12421 cgcaggtttg gtttcggctc ggctgatccg gatgggtgtt caagcgtcga acaccgcgat
    12481 cgcacgtgca catgtgcaat gcccttcgcg cgtcatgctg cgtgatgtca tccacaactg
    12541 cgagatcccg gcactcggcg tccggatcag acgtcgccat cgcgtgatcg ggcgcccggc
    12601 gatcaggtgg tcgccagctc gtggacgatg gcggccatcg ttgcccgcgc gcggccgatc
    12661 tcgtcgggct cgagcgtgac gccgccgacc gccgcgtggc cgccgccgcc gaaccgcgcg
    12721 cacagcgcgc cgatgtcgtg ccgccggcgc tgcttgctcc acgggttgac cccgaccgag
    12781 atcttgatgt tctggctgtg ctgcgtcccc gacaccgcgt agaggcagct cggatagagc
    12841 aggtagccga ggaagcccgg gctgcgcgcg ccggggtcag cgaaccggtc gaacacgatg
    12901 acgtcgccgt gccacacgcc gagccgtcgg atctcctcga ggtcgcgcgc gcgctcggac
    12961 tcgaccacag cgagcgccac cgcgacctcc ggacgcgcgg tgatctgggc gagcgactcg
    13021 cgcgacagcc actcgacata gtgcgcggtg tcgaccggcg tccggccgtg cgcgagccac
    13081 gccgcgatgc gctgggccgg cagggtcagc gcgaccgcat cctcggcgga cgcgaaccgc
    13141 gcggcgtcga tcgtgtcggc ccaccgcgtc gcctcgacca gatgaggtgg cggcttccac
    13201 ctccacccgg cctcgagctc gcgggcgatc agcccggcgc acgacagcgc ggccgggtcg
    13261 aagaaccagg tcgggcgatg ctcgcgctcg aacacctcgc gcagcgccgg gggctggaac
    13321 gccgtgggat gatgatcgaa ccaccagcgc atccgggagt cggcacagaa ccggaaatcc
    13381 acgcaggcgt ggtcgtcggc atcgagcggc accccctcga acgggttgcc gtcccggtgg
    13441 accatcccga ccggcaggac ccggaccccg cggtcgagca cgtcgcggta gaacgcggcg
    13501 aacaacgccg ccgaggtcgt gccgtcgaag cacgcgtcgt ggaagaagat cttgaggacg
    13561 cgtgccacga tggctggccc atcataccgc ggtccgccgg tcagcggcac atcgacgtag
    13621 gtgttacctt cgcatccatg caccagtggc tactgagcgc tcacctcatc ggcgtgatct
    13681 tctggctagg ctgcctgttt gcagtgtact ggctgctccg ggttcacgcc caggcgccca
    13741 ggaacgtgct cgacaagctc acgctcatgg agcgctccct cgccctcacg atggacctcg
    13801 ccgccgcgct ggcgatcggc accggcctgg cgatggcgct ccgccacgac atcgaccccg
    13861 tccacacgct gttcaccaag cccggcgcgg gctggttcca catcaagctg acggtcgtcg
    13921 tgctcggcat tctcccggtc cacggcatgg tgcgcggcaa gatcgccaag ttcagccgcg
    13981 gcgtgatctc gaacgtcccg cagtggatat ggagcctgct cctgatctcg atcgtcatca
    14041 tcgtggtgat ggtgatccgc ggaccgctga tgttcgcctc gtcgacctag ccgggaccta
    14101 gccgggacct ctccggatcc cgccgggtct tgccgggatc cggatctgat cggtgttttt
    14161 gggttcccag accggggcgg tcgcgtgtca ccaggaaaac cgaaaggatt ccatgcaccg
    14221 ttccctggt
//
LOCUS       DASVOR010001592         4852 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_162181,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001592 DASVOR010000000
VERSION     DASVOR010001592.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162181"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1025
                     /locus_tag="VF516_29910"
     CDS             <1..1025
                     /locus_tag="VF516_29910"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8111999.1"
                     /translation="ARESPAARESPAAREATMRDVSQKLRRLLYIVPYVAKHPEGVAV
                     DQLAKMLGVERDELLADLELLSQVGPPDGDPGEYLLVSVDEGRVFVDLAHRLTRPLRL
                     TPAEGAALLLGLRSLRESGIAPFDAAMQSAENKLLAALGRDAGEAESLATSTIVAEPD
                     AAVAGHLRALVTAARRSQRIEIDYAAVSRAHAERRPIDPYGIVHHAGEWYVVGFCHKR
                     GDVRTFRIDRIAALHQTGDSFERPASFDLEAYRRDRLYVPSADSVTVRVQLDPLAVTR
                     IGANWPVGEITMQDDGSAELLVDCDGFEWVTSWVLGLGRHAWIVGPAAARQAMRERIA
                     RLRELA"
     gene            complement(1102..2082)
                     /locus_tag="VF516_29915"
     CDS             complement(1102..2082)
                     /locus_tag="VF516_29915"
                     /inference="COORDINATES: protein motif:HMM:NF039709.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HEX8112000.1"
                     /translation="MRLLFLLAALAACSSGTSKPPAAPQPPESKAAARPAASTSADPS
                     QGAETRPAMPTGPAEMKISPAQTTVKLVSDGQGTKQVLRYSARPGARQTVEVAMDFTG
                     RQDTEEATAPTIVLTCDARTGAVDKNGAADYTLTVTGADARPVAGSDRTPQDLKPALA
                     GLTGLTIAGKRSANGAIGETTLRLAHPPASSDDALGLIRETLPVLPVLPTQPVGVGAK
                     WQSTTAARLADKFDITQVTDYEVVAHDGASWTIKGTTKVSGKDQKIDNAMISAISGSG
                     TSETTIADGAVYPAHKEQLETTFKASDPESADPGKAVQFTIKVGGTVTPK"
     gene            2222..4138
                     /gene="treZ"
                     /locus_tag="VF516_29920"
     CDS             2222..4138
                     /gene="treZ"
                     /locus_tag="VF516_29920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX8112001.1"
                     /translation="MPARRLPIGAEVVDRGAHVRVWAPGHRNVTLVIESGTRDGPGEV
                     MLDPASDGYHAGFAPGLVAGARYRFRLGDDPALHVDPASRYQPDGPFGPSQMIDPAAF
                     RWTDAGWTGVAPDRHVLYELHLGTFTPEGTWNAAAAWLGYLADLGITTLELMPVADFA
                     GRHNWGYDGVNLFAPSRRYGTPDDMRRFVDRAHGVGLAVILDVVYNHFGPAGNPLPAW
                     TPAYESDRCSEWGAAFNFDGKASAPVRELVTANAAYWIDEFRLDGLRIDATQQLFDRS
                     PDHVLAAIARAARAAGRGRRIFLVGENEPQDARLLDPPFELDALWNDDFHHSARVAAT
                     GLIEGYLHDYRGTAQELVSAVKRGFLYQGQLYPWQGKPRGRSTRGVARHRLVHFLENH
                     DQCANAGFGERLVTLTDPATLRALTAVLLLGPELPMLFQGQEHGATEPWRFFADHAPP
                     LGAAVRDGRARFVAQFARLATPEAQAALADPTAEATFRGCVLDPRHRRLDHPLVAMHR
                     DLIELRHRDPAFTDPRPDALDGAVLCDRAFVVRYFQPDPRGDRLLVVNLGPTYASLTT
                     AEPLVAPPDGTGWQLVWSSEDPRYGGHGTPPPFHRMQLAIPARAAVVLAPDPEAARWD
                     EPPPGEPRAPEPRR"
     gene            4135..>4852
                     /locus_tag="VF516_29925"
     CDS             4135..>4852
                     /locus_tag="VF516_29925"
                     /inference="COORDINATES: protein motif:HMM:NF023855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching enzyme N-terminal
                     domain-containing protein"
                     /protein_id="HEX8112002.1"
                     /translation="MTAVSLPVDDRLFPREWLVCTGLGGYASGTIGGAPTRRYHGYLI
                     AALPNPAGRVMMLNALLEQVRLPDGSRADLGWDGPAFAGGPRDVRHGGRIPLVNFRLE
                     LGLPIWHYAGHGVVIERRIVLSYSQNTALLVYRLLEGAPIRLELRPALQFRGHDEPVS
                     TMIPEAYPLHAFGARIELTAPAPLPPLKLHLAGERTSFVIEPRAVPDIAYITEESRGY
                     ASRGRLYSPGRFRAHLAPGGE"
BASE COUNT          588 a         1817 c         1775 g          672 t
ORIGIN      
        1 cggcccggga gagcccggcg gcccgggaga gcccggcggc ccgggaggcg acgatgcgtg
       61 acgtgtcgca gaagctgcgc cggctgctct acatcgtgcc gtacgtcgcc aagcaccccg
      121 agggcgtcgc ggtcgaccag ctggcgaaga tgctcggcgt cgagcgcgac gagctgctcg
      181 ccgacctcga gctgctgtcg caggtcggcc cgcccgacgg cgaccccggc gagtacctgc
      241 tggtctcggt cgacgagggc cgcgtgttcg tcgacctcgc gcaccggctg acccggccgc
      301 tgcggctgac ccccgccgag ggcgccgcgc tgctgctcgg gctgcgctcg ctgcgcgaga
      361 gcgggatcgc gccgttcgac gccgcgatgc agtcggccga gaacaagctc ctggccgcgc
      421 tcggccgcga cgccggcgag gccgagagcc tggcgaccag cacgatcgtc gccgagcccg
      481 acgccgcggt cgccggccac ctgcgcgcgc tggtcaccgc cgcgcgccgg agccagcgga
      541 tcgagatcga ctacgccgcc gtgtcgcgcg cccacgccga gcgccggccg atcgacccct
      601 acggcatcgt ccaccacgcc ggcgagtggt acgtcgtcgg gttctgccac aagcgcggcg
      661 acgtccggac gttccggatc gaccggatcg ccgcgctgca ccagaccggc gacagcttcg
      721 agcgccccgc gtcgttcgat ctcgaggcct accgccgcga ccggctgtac gtgccgtcgg
      781 ccgactcggt caccgtgcgg gtccagctcg atccgctcgc ggtcacccgg atcggggcca
      841 actggccggt cggcgagatc accatgcagg acgacggctc cgccgagctg ctggtcgatt
      901 gcgacggctt cgagtgggtc accagctggg tgctcggcct gggccgccac gcctggatcg
      961 tcggacccgc cgccgcgcgc caggcgatgc gcgagcgaat cgcccggctg cgcgagctcg
     1021 cctgagcgag atcggagcga gatcggagcg aggccggagc gggaccggag cgaggccgga
     1081 gcgaggccgg agcgggaccg atcacttcgg cgtgacggtg ccgccgacct tgatcgtgaa
     1141 ctggaccgcc ttgccgggat cggccgactc ggggtcggac gccttgaacg tcgtctcgag
     1201 ctgctccttg tgggccgggt acaccgcgcc gtcggcgatc gtggtctcgc tggtgccgga
     1261 cccggagatc gccgagatca tcgcgttgtc gatcttctga tccttgccgc tgaccttggt
     1321 ggtgcccttg atcgtccagc tcgctccgtc gtgggcgacg acctcgtaat cggtgacctg
     1381 ggtgatgtcg aacttgtcgg cgagccgcgc cgcggtggtc gactgccact tcgcgccgac
     1441 gccgaccggc tgggtcggca gcaccggcag caccggcagg gtctcgcgga tcaggccgag
     1501 cgcatcgtcg ctgctggcgg gtgggtgcgc gagccggagc gtggtctcgc cgatcgcgcc
     1561 gttcgcgctg cgcttgccgg cgatggtcag cccggtgagc ccggcgagcg ccggcttgag
     1621 gtcctggggc gtgcgatccg acccggcgac cgggcgggcg tcggccccgg tgacggtgag
     1681 cgtgtagtcg gcggcgccgt tcttgtcgac cgccccggtc cgggcgtcgc aggtcagcac
     1741 gatcgtcgga gcagtcgcct cctccgtgtc ctgccggccg gtgaagtcca tcgcgacctc
     1801 gacggtctgc cgggcccccg ggcgggcgct gtagcgcagc acttgcttgg tgccctggcc
     1861 gtccgacacc agcttcacgg tggtctgcgc cggcgagatc ttcatctcgg ccggaccggt
     1921 cggcatcgca ggccgggtct ctgccccctg ggatgggtcg gccgacgttg acgccgccgg
     1981 tctggccgcg gccttggact ccggaggctg cggggccgcg ggcggcttgg aggtgccgga
     2041 gctgcacgcg gcgagggcgg cgaggaggaa cagcaggcgc attgggggac gctaccatcc
     2101 gggttcaggg cggtcgagca gcacatccgc gcccggtggg cgcgcgcgct cgcgggcgcc
     2161 gacgacgctc ccggctccgc cgagccgcgc ggtggcatat cgagtgcacc gccgcgaaga
     2221 gatgcccgcg cgcagactcc cgatcggggc cgaggtcgtg gaccgcggtg cccacgtccg
     2281 ggtctgggcg ccggggcatc gcaacgtgac attggtcatc gaatcgggga cgcgcgacgg
     2341 gccgggcgag gtcatgctcg acccggcgtc cgacggctac cacgccggct tcgcaccggg
     2401 gctggtcgcc ggagcgcgct accggttccg gctcggcgac gatccggcgc tgcacgtcga
     2461 cccggcgtcg cgctaccagc ccgacggacc gttcgggccg tcgcagatga tcgacccggc
     2521 ggcgttccgg tggaccgacg cgggatggac cggcgtcgcg ccggaccgcc acgtgctgta
     2581 cgagctgcac ctgggcacgt tcacgcccga gggcacctgg aatgcggcgg cggcgtggct
     2641 cggctacctc gccgacctcg ggatcacgac gctcgagctc atgccggtcg ccgacttcgc
     2701 cggccggcac aactggggct acgacggcgt caacctgttc gcgccgtcgc ggcgctacgg
     2761 cacgcccgac gacatgcggc ggttcgtcga ccgcgcccac ggcgtcgggc tcgcggtgat
     2821 cctcgacgtg gtctacaacc acttcgggcc ggcgggcaac ccgctgcccg cgtggacgcc
     2881 ggcctacgag tccgaccgct gcagcgagtg gggcgcggcg ttcaacttcg acggcaaggc
     2941 cagcgcgccg gtgcgcgagc tggtgaccgc gaacgccgcc tactggatcg acgagttccg
     3001 cctcgacggg ctgcggatcg acgcgaccca gcagctgttc gaccgctcgc ccgaccacgt
     3061 gctcgccgcg atcgcccgcg ccgcgcgcgc cgccggccgc ggccgccgga tcttcctcgt
     3121 cggcgagaac gagccgcagg acgcgcggct gctcgatccg ccgttcgagc tcgacgcgct
     3181 gtggaacgac gacttccacc acagcgcgcg ggtcgccgcc accgggttga tcgagggcta
     3241 cctccacgac taccgcggca ccgcccagga gctggtgtcg gcggtcaagc gcggcttcct
     3301 ctaccagggc cagctctacc cgtggcaggg aaagccgcgc ggccggtcga cccgcggcgt
     3361 cgcgcgccac cgcctggtcc atttcctgga gaaccacgat cagtgcgcca acgccgggtt
     3421 cggcgagcgg ctggtcacgc tgaccgatcc ggcgacgctg cgcgcgctga ccgcggtgct
     3481 gctgctcggc cccgagctgc cgatgctgtt ccagggccag gagcacggcg cgaccgagcc
     3541 gtggcggttc ttcgccgatc acgccccacc gctgggcgcc gcggtgcgcg acggccgggc
     3601 gcggttcgtc gcgcagttcg cgcggctcgc cacgcccgag gcccaggccg cgctcgccga
     3661 cccgaccgcc gaggcgacgt tccgcggctg cgtgctcgat ccgcgccacc gccggctcga
     3721 ccacccgctg gtcgcgatgc accgagatct gatcgagctg cgccaccgcg atccggcgtt
     3781 caccgacccg cggccggacg cgctcgatgg cgccgtgctg tgcgatcgcg cgttcgtcgt
     3841 gcgctacttc cagcccgatc cgcgcggcga tcgcctgctg gtggtcaacc tgggcccgac
     3901 ctacgcgtcg ctgaccaccg ccgagccgct ggtggcgccg cccgatggca ccggctggca
     3961 gctggtgtgg tcgagcgagg acccgcgcta tggcggccac ggcacgccac cgccgtttca
     4021 ccgcatgcag ctggcgatcc cggcccgcgc cgcggtcgtg ctcgcgcccg accccgaggc
     4081 cgcgcggtgg gacgagccgc cgccgggtga gccgcgcgcg ccggagcccc gacgatgacc
     4141 gcggtctcgc tccccgtcga cgatcggctg ttcccccgcg agtggctggt gtgcaccggc
     4201 ctgggcggct acgcgtccgg cacgatcggc ggcgcgccga cccggcgcta tcacggctac
     4261 ctgatcgccg cgctgcccaa ccccgccggc cgggtgatga tgctcaacgc gctgctcgag
     4321 caggtccggc tgcccgacgg ctcgcgcgcc gacctcggct gggacggccc ggcgttcgcc
     4381 ggtggaccgc gcgacgtgcg ccatggcggt cggatcccgc tggtcaactt ccgcctcgag
     4441 ctcggcctgc cgatctggca ctacgccggc catggcgtgg tgatcgagcg ccggatcgtg
     4501 ctgtcgtaca gccagaacac ggcgctgctg gtctaccgcc tgctcgaggg tgcgccgatc
     4561 cggctcgagc tgcggcccgc gctgcagttc cgcggccacg acgagccggt gtcgaccatg
     4621 atccccgagg cctacccgct gcacgcgttc ggcgcgcgga tcgagctcac cgcgccggcg
     4681 ccgctgccac cgctcaagct ccacctcgcc ggcgagcgca cgtcgttcgt gatcgagccc
     4741 cgcgcggtgc ccgacatcgc atacatcacc gaggagagcc gcggctacgc gtcgcgcggc
     4801 cggctgtaca gccccggccg gttccgcgcc cacctggcgc ccggcggcga gg
//
LOCUS       DASVOR010001593         3586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_337432,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001593 DASVOR010000000
VERSION     DASVOR010001593.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337432"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1582
                     /locus_tag="VF516_29930"
     CDS             <1..1582
                     /locus_tag="VF516_29930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HEX8112003.1"
                     /translation="ARYISGFSCEKGTVENVDALKVLNKERQKRMKQFPNLVDYEAKL
                     AFKSFIDALGSPKMPEAGAPVDDVVVKKTLLGAVRRLQIKRAFRRASADVWAKRREVR
                     IGIPRVLNLYSTGPFWRTYFEVLGVDSRNVVFSDETGEEMWQAGCKYGSVDPCYPSKV
                     CQAHIHNLLFKHHEKKPLNYIFFPSITHIPTFIVNQMDTASCPVVSGTPKVIRAAFTK
                     ETDFFAERGISYLDTAVTLNEPLMCKKQMFDEWGPLLGLTADENDFAIDQAFKAVQAF
                     EDEMERKGKEILGEVMRENRVALVMLGRPYHNDPGLNHSVLEEFQALGYPILSMRSLP
                     RDPETIRELFAEDLAKGIIQSGLDVSDVWPENYSTNSVQKVWAAKYAARHPNLACLDL
                     SSFKCGHDAPTYGLIDSIIASAGKAYSALHDIDANKPSGSIKIRVKTYAHTLMLLREK
                     LEDRAAKQVELDRAVTERKLEMMLALQSKLSGVGRTDDRLDREIAQLGERVAAWQAAD
                     EAARPKGKRAAALNVMRQ"
     gene            1625..3481
                     /locus_tag="VF516_29935"
     CDS             1625..3481
                     /locus_tag="VF516_29935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyglutaryl-CoA dehydratase"
                     /protein_id="HEX8112004.1"
                     /translation="MSTTNDNNGSNLDAMSIEAIEAELKQFELEERKRLGLPVEGARH
                     WFDEVPRTFTRDQREHTTILVSGLTMAHDLFVQAALRGIGYKVLAMDVPDNDALQYGR
                     EYGNRGQCNPTYFTVGNLVKYLDGLRSRGMSKDDIVKNHVFITAGACGPCRFGTYVTE
                     YRKALRDSGFDGFRVLLFQQTGGLKQATGDGVGLEMNPTFFWGVVRAIMIGDVLNALG
                     YRIRPYEVLPGAADRALEISKKIIADAFEHNTSLPIALFKVRRELGRVKVDRTKVKPR
                     VGIIGEFWAMTTEGDGNYGLQRFLEAEGAEPDIQIVTAWLLYMIWQGRYDTVQRSELR
                     GVDAGKAEGGSKFSLKGVDVRKRRASLWAGEKVLRGLFQTVSTAMGLNDYHLPDMEEI
                     AEVSHAFYDNNLRGGEGHMEIGKLIQNVAYAKVNMTLSVKPFGCMPSSSVSDGVQSFI
                     TELYPQGIFLPIETNGDGAVNVYSRVQMQLFKAKQQAQKEVDKALADVGMTMDEVRAY
                     LAKHPAMMSPFHRAPHKAGCTAADLVYEVGARKNRFTYLKERVIAQVTGKAERSHAEA
                     HDRLIQIARNAAAKTRKKNSIVSVAPELDPATVEDAARPPVPAGRKSLKVVN"
BASE COUNT          669 a         1226 c         1182 g          509 t
ORIGIN      
        1 cgcgcgctac atcagcgggt tctcgtgcga gaagggcacg gtcgagaacg tcgacgcgct
       61 caaggtgctc aacaaggagc gccagaagcg catgaagcag ttccccaacc tggtcgacta
      121 cgaagccaag ctcgcgttca agagctttat cgacgcgctg ggctcgccga agatgccgga
      181 ggccggcgcg ccggtcgacg acgtcgtggt caagaagacc ctgctcggtg cggtccgccg
      241 tctacagatc aagcgcgcct tccggcgcgc ttctgccgat gtctgggcca agcggcgcga
      301 ggtccggatc ggcatcccgc gcgtgctcaa cctgtactcg accggcccgt tctggcggac
      361 ctacttcgag gtgctcggcg tcgactcgcg caacgtggtg ttctccgacg agaccggcga
      421 ggagatgtgg caggccggct gcaagtacgg ctcggtcgat ccgtgctacc cgtcgaaggt
      481 ctgccaggcc cacatccata acctgctgtt caagcaccac gagaagaagc cgctcaacta
      541 catcttcttc ccgtcgatca cccatatccc gacgttcatc gtcaaccaga tggacaccgc
      601 gagctgtccg gtggtgtcgg gcacgcccaa ggtgatccgc gcggcgttca ccaaggagac
      661 cgacttcttc gccgagcgcg ggatcagcta cctcgacacc gcggtcacgc tcaacgagcc
      721 gctgatgtgc aagaagcaga tgttcgacga gtggggcccg ctgctcgggc tcaccgccga
      781 cgagaacgac ttcgcgatcg accaggcgtt caaggcggtc caggcgttcg aggacgagat
      841 ggagcgcaag ggcaaggaga tcctcggcga ggtgatgcgc gagaaccggg tcgcgctggt
      901 catgctcggc cggccgtacc acaacgaccc cgggctcaac cactcggtgc tcgaggagtt
      961 ccaggcgctc ggctacccga tcctgtcgat gcggtcgctg ccgcgcgatc ccgagacgat
     1021 ccgcgagctg ttcgccgagg atctcgccaa gggcatcatc cagagcgggc tcgacgtgtc
     1081 cgacgtgtgg cccgagaact actcgaccaa ctcggtccag aaggtgtggg ccgccaagta
     1141 cgccgcgcgc caccccaacc tggcgtgcct cgacctgtcg agcttcaagt gcggccacga
     1201 cgcgccgacc tacggcctga tcgattcgat catcgcgtcg gccggcaagg cgtactcggc
     1261 gctgcacgac atcgacgcca acaagccatc cgggtcgatc aagatccggg tcaagaccta
     1321 cgcccacacc ctgatgctgc tgcgcgagaa gctcgaggac cgcgccgcca agcaggtcga
     1381 gctcgaccgc gcggtcaccg agcggaagct cgagatgatg ctcgcgctgc agagcaagct
     1441 gtccggcgtc ggccgcaccg acgaccggct cgatcgcgag atcgcgcagc tcggcgagcg
     1501 ggtggcggcg tggcaagcgg ccgacgaggc cgcccggccc aagggcaagc gggcggcggc
     1561 cctcaacgtg atgcggcaat gatctgattt cccgttcctt cagctcccac cttcggagaa
     1621 agccatgtcc acgacgaacg acaacaacgg aagcaacctc gacgcgatgt cgatcgaggc
     1681 gatcgaggcc gagctgaagc agttcgagct cgaggagcgc aagcgcctcg gcctgccggt
     1741 cgagggcgcc aggcactggt tcgacgaggt gccgcgcacg ttcacgcgcg accagcgcga
     1801 gcacaccacg atcctggtgt cggggctgac catggcgcac gacctgtttg tccaggcggc
     1861 gctgcgcggc atcggctaca aggtcctcgc gatggacgtg ccggacaacg acgccctgca
     1921 gtacgggcgc gagtacggca accgcggcca gtgcaacccg acgtacttca ccgtcggcaa
     1981 cctggtgaag tacctcgacg gcctgcgcag ccgcgggatg tccaaggacg acatcgtcaa
     2041 gaaccacgtg ttcatcaccg ccggcgcgtg tggcccgtgc cggttcggca cctacgtcac
     2101 cgagtaccga aaggcgctgc gcgactccgg gttcgacggg ttccgcgtgc tgctgttcca
     2161 gcagaccggc gggctcaagc aggcgaccgg cgacggcgtc ggcctggaga tgaacccgac
     2221 gttcttctgg ggtgtggtcc gcgcgatcat gatcggcgac gtgctcaacg cgctcggcta
     2281 ccggatccgg ccctacgagg tcctgcccgg cgcggccgac cgcgcgctcg agatcagcaa
     2341 gaagatcatc gccgacgcgt tcgagcacaa cacgtcgctg ccgatcgcgc tgttcaaggt
     2401 ccgccgcgag ctcggcaggg tcaaggtcga ccgcaccaag gtcaagccgc gggtcggcat
     2461 catcggcgag ttctgggcga tgaccaccga gggcgacggc aactacggcc tgcagcgctt
     2521 cctcgaggcc gagggcgccg agcccgacat ccagatcgtc accgcgtggc tgctctacat
     2581 gatctggcag ggccgctacg acaccgtcca gcgctccgag ctgcgcgggg tcgacgccgg
     2641 caaggccgag ggcggcagca agttcagcct caagggcgtc gacgtccgca agcgccgcgc
     2701 gtcgctgtgg gccggcgaga aggtgctgcg cggcctgttc cagaccgtgt cgaccgcgat
     2761 ggggctcaac gactaccacc tgccggacat ggaggagatc gccgaggtct cgcacgcgtt
     2821 ctacgacaac aacctgcgcg gcggcgaggg ccacatggag atcggcaagc tgatccagaa
     2881 cgtggcctac gccaaggtca acatgaccct gtcggtcaag ccgttcggct gcatgccgag
     2941 ctcgtcggtg tccgacggcg tccagtcgtt catcaccgag ctctacccgc agggcatctt
     3001 cctgccgatc gagaccaacg gcgacggcgc ggtcaacgtc tacagccggg tccagatgca
     3061 gctgttcaag gccaagcagc aggcccagaa ggaggtcgac aaggcgctgg ccgacgtcgg
     3121 catgacgatg gacgaggtcc gcgcctacct cgccaagcac ccggcgatga tgagcccgtt
     3181 ccaccgcgcc ccgcacaagg ccggctgcac cgcagccgac ctggtgtacg aggtcggcgc
     3241 gcgcaagaac cggttcacgt acctcaagga gcgggtgatc gcccaggtca ccggcaaggc
     3301 cgagcgcagc cacgccgagg cccacgaccg gctgatccag atcgcgcgca acgcggcggc
     3361 caagacccgc aagaagaact cgatcgtcag cgtcgcgccc gagctcgacc cggccaccgt
     3421 cgaggatgcc gcgcgaccgc cggtcccggc cggccgcaag agcctcaagg tcgtgaacta
     3481 ggccatgtcg ctgcgcgatc ggctcgacca gctgcgcgcc gaggcgcatc gcgcgatcga
     3541 caagctgccc ggcaaggcgg gcgagctcgc gcgcaaggcc aacacc
//
LOCUS       DASVOR010001594         2243 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_191958,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001594 DASVOR010000000
VERSION     DASVOR010001594.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2243
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191958"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..433
                     /locus_tag="VF516_29940"
     CDS             71..433
                     /locus_tag="VF516_29940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112005.1"
                     /translation="MQLYSYFFLPGPLDTALFAQWAVEREPALGFAHYLLGLQRQFQG
                     KWDESARELDRALVLGLPGPHFVRNAARRLAVAAYHSHDTARVEQAIAVLSRPAMTTA
                     DHLLARDWADRLAFDAKR"
     gene            complement(430..1869)
                     /locus_tag="VF516_29945"
     CDS             complement(430..1869)
                     /locus_tag="VF516_29945"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8112006.1"
                     /translation="MSSRILRAALCLICVASLLGPRTKHARADGRATDTYLDQTRERW
                     ERVARQIWDTPETGLKETRSSAALIDLLQKEGFQVTRGVGGEPTAFVATAGSGQPVIG
                     LLAEYDALPGLSQSAGEAKKHAVSDGAPGHGCGHNLLGTASVAAAIAANRERIARKLP
                     GTIQLFGTPAEEILFGKTFMIRGGAFDHTGVVFAWHPDDQNRVTNRTRLAVAAANVEF
                     FGRSAHAAAAPWLGRSALDALTLFDHAIALMREHIKPTARIHRMIREGGVAPNIIPDY
                     SLGEYWIRDATGDSVNEMMERLKKAAEGAALATETRAKVTLTFSTREPVPNDTLDAVL
                     QKELERIGPPKFDDTDQKFARAMQKELGFAELGMATAVMPYAQKNGGGASSDIGEVSA
                     AVPLAELNLATRPLGTAAHHWAQTACAAHPVGYKGMGVAAKILAASTIDALTEPGIVK
                     SARDEFAVQTKGKPYVSPLPPDAKPVAPH"
     gene            1868..2080
                     /locus_tag="VF516_29950"
     CDS             1868..2080
                     /locus_tag="VF516_29950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112007.1"
                     /translation="MFASEGGERSATGGGLPAGAPHIDRRFTRLFRTVPGDWRGRPSG
                     QVLPESERDQPGDIADAGHAAAALPL"
BASE COUNT          257 a          808 c          845 g          333 t
ORIGIN      
        1 gctgccgccc gacgccaagc cggtcgcccc gcactagcgg ttcgcgctcg cgcaccaggg
       61 gccggccgcg gtgcagctgt actcgtactt cttcctgccc gggccgctcg acacggcgct
      121 gttcgcgcag tgggcggtcg agcgcgagcc cgcgctcggc ttcgcgcact acctgctcgg
      181 gctgcagcgc cagttccagg gcaagtggga cgagtcggcg cgcgagctgg atcgcgcgct
      241 cgtgctcggg ctgccggggc cgcacttcgt gcgcaacgcg gcgcgccggc tcgcggtcgc
      301 ggcgtaccac agccacgaca ccgcgcgggt cgagcaggcg atcgcggtgc tgtcgcggcc
      361 cgcgatgacg acggccgacc acctgctggc gagggactgg gccgatcggc tggcgttcga
      421 cgcgaaacgc tagtgcgggg cgaccggctt ggcgtcgggc ggcagcggcg agacgtacgg
      481 cttgcccttg gtctgcaccg cgaactcgtc gcgcgccgac ttgacgatgc ccggctcggt
      541 cagcgcgtcg atcgtgctcg cggcgaggat tttggcggcg acgcccatgc ccttgtagcc
      601 gaccgggtgc gccgcgcacg cggtctgcgc ccagtggtgg gccgcggtgc cgagcggccg
      661 ggtcgcgagg ttgagctcgg cgagcggcac ggccgcgctg acctcgccga tgtccgacga
      721 cgcgccgccg ccgttcttct gcgcgtacgg catcacggcg gtcgccatgc cgagctcggc
      781 gaacccgagc tccttctgca tcgcccgggc gaacttctgg tcggtgtcgt cgaacttcgg
      841 cggcccgatc cgctcgagct ccttctgcag caccgcgtcg agcgtgtcgt tgggcaccgg
      901 ctcgcgggtc gagaacgtga gcgtgacctt ggcgcgggtc tcggtggcga gcgccgcgcc
      961 ctcggcggcc ttcttgagcc gctccatcat ctcgttgacg ctgtcgccgg tcgcgtcgcg
     1021 gatccagtac tcgccgagcg agtagtcggg gatgatgttg ggcgccacgc cgccctcgcg
     1081 gatcatgcgg tggatgcgcg cggtcggctt gatgtgctcg cgcatcagcg cgatcgcgtg
     1141 atcgaacagg gtcagcgcgt cgagcgcgct gcggccgagc cacggcgcgg cggcggcgtg
     1201 cgccgagcgg ccgaagaact cgacgttggc ggcggcgacc gcgagccggg tccggttggt
     1261 gacgcggttc tggtcgtcgg gatgccaggc gaacacgacg ccggtgtggt cgaacgcgcc
     1321 gccgcggatc atgaacgtct tgccgaacag gatctcctcg gccggggtgc cgaacagctg
     1381 gatcgtcccg gggagcttgc gcgcgatccg ctcgcggttg gcggcgatcg cggcggcgac
     1441 gctggcggtg ccgagcaggt tgtggccgca gccgtggccc ggcgcaccgt cgctgaccgc
     1501 gtgcttcttc gcctccccgg ccgactgcga caggccgggc agcgcgtcgt actcggcgag
     1561 caggccgatc accggctggc cggagccggc ggtggcgacg aacgcggtcg gctcgccgcc
     1621 gacgccgcgc gtgacctgga agccctcctt ctggagcagg tcgatcagcg ccgccgagga
     1681 ccgggtctcc ttgagcccgg tctcgggcgt gtcccagatc tggcgggcga cgcgctccca
     1741 gcgctcgcgg gtctggtcga gataggtgtc ggtggcgcgc ccgtcggcgc gggcgtgctt
     1801 cgtgcgcggg ccgagcagac tggcgacgca gatcaggcag agcgcagcgc gtaggatccg
     1861 tgatgacatg ttcgcctccg agggcggcga acgtagcgcc accggcggcg gcctgccagc
     1921 cggcgcgccg cacatcgatc gccggttcac ccggctgttc cggaccgtcc ccggcgactg
     1981 gcgcggccgg cccagcggcc aggtgctgcc cgagtccgag cgcgaccagc ccggggacat
     2041 cgccgacgcc ggccatgcgg ccgccgcgct gccgctgtag acttccggcc gttgccccga
     2101 ccctgcgcac cgaaccgtct cgcggaggcc gtcccgtgac cgccgcggcc gcccccgacc
     2161 tcgtccggct cgatgcccgc cggctgtccg agctgatcca cgcgcgccgg gcgtcgtgcg
     2221 tcgaggtcat gaccgcctac ctc
//
LOCUS       DASVOR010001595         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_513098,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001595 DASVOR010000000
VERSION     DASVOR010001595.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513098"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..750)
                     /locus_tag="VF516_29955"
     CDS             complement(121..750)
                     /locus_tag="VF516_29955"
                     /inference="COORDINATES: protein motif:HMM:NF020376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HEX8112008.1"
                     /translation="MCSWWNPVDVRSSGEFRFWYEDDLTTAQQRGFRMLPEFPAHRRA
                     EDPYVERYLFPLFAERIPAPTRRDAAAMLAAWGVEHLDDQFEVLARSGGLRATDRLEL
                     AEYRAADDDLSDPLEFRIAGRRHLVDVAPLAVGDAVSLQREPDNAADPAAVVVDRLGR
                     RAGYVPRQYTRLLGGLLDGGVALRGNVIRELVLPEDVGRLVVQVTASRT"
     gene            complement(790..1527)
                     /locus_tag="VF516_29960"
     CDS             complement(790..1527)
                     /locus_tag="VF516_29960"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX8112009.1"
                     /translation="MYDLANVFAAQIRTAETEASRVYEPVIGIVTDNKDPSKLGRVKL
                     KIPILSDQDTTYWCPIIMLGAGKNRGWFFIPDKDDEVLVMFEHGDLNRPLVVGALWNG
                     KDKPPDKNPGGNPRRVLKSRLGSKITFDDDKKQLIIEDGVGKGKITFDSQANKITIEA
                     IDGDVCFQTPADDMTIVAQSIEMTANTNLEIHAGDAMQWGAGGSATIKISGTTLSGSK
                     VNTNCGSAQMPNAPTTSPQDIPDPYGS"
     gene            complement(1546..>1851)
                     /locus_tag="VF516_29965"
     CDS             complement(1546..>1851)
                     /locus_tag="VF516_29965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112010.1"
                     /translation="HPTAAPAPSRILRTALRATAGGAFSTERWKAGDYIRERFPLTIP
                     PDWSPAGQGAIGLGLVAEDAAGKIHTPTGDRLASDPGIAVLGTIPTTAPGSSLPPRP"
BASE COUNT          286 a          578 c          668 g          319 t
ORIGIN      
        1 gatcgatgcc gccgaggagc gcatcaccga cggcgccgcg gccgacggcg cggccgggct
       61 gctgcgctcc ctcgatacgc ggatccacct gtacgtccag aagaacgcgc ccgacgtacg
      121 ctaggtacgg ctggcagtga cctgcaccac gagccgacca acgtcttctg gaaggacgag
      181 ctctcgaatc acgttgccgc gaagagcgac accgccgtcg agcaagccac cgagaagccg
      241 agtgtactgg cgaggcacat agccggctcg ccgacccagg cgatccacaa ccaccgctgc
      301 tggatcggca gcgttgtccg gctcgcgttg caaggagacc gcgtcgccca ccgcgagggg
      361 agccacatcc acgaggtggc ggcggccggc gatccggaac tccagggggt cggagagatc
      421 atcgtcggcc gctcgatact cggccaactc gaggcggtcg gtggctcgta aaccaccgct
      481 gcgcgcgagc acctcgaact gatcgtcgag atgttcgact ccccaagctg ccagcatcgc
      541 tgcagcatcg cgacgcgtgg gcgcagggat tcgctctgca aacagcggaa agagataacg
      601 ttcgacgtac ggatcctctg cacgccggtg cgcggggaac tcgggcagca tccggaagcc
      661 gcgctgctgt gcggttgtga gatcgtcttc gtaccagaaa cggaactctc cggacgacct
      721 gacgtcaacc gggttccacc aggagcacac ccggtgatgc tacgccacgc gcggcggtcg
      781 cgctccagct cacgacccgt aggggtcggg gatgtcttgc gggctggtgg tcggggcgtt
      841 cggcatctgc gccgagccgc agttggtgtt gaccttggag ccggacagcg tggtgccgga
      901 gatcttgatg gtggcgctgc cgccggcgcc ccactgcatc gcgtcgccgg cgtggatctc
      961 gaggttggtg ttggcggtca tctcgatcga ctgggcgacg atggtcatgt cgtcggccgg
     1021 ggtctggaag cagacgtcgc cgtcgatcgc ctcgatcgtg atcttgttgg cctggctgtc
     1081 gaaggtgatc ttgcccttgc cgacgccgtc ctcgatgatc agctgttttt tgtcgtcgtc
     1141 gaaggtgatc ttcgagccca ggcgcgactt gaggacgcgg cgcgggttgc cgccggggtt
     1201 cttgtcgggc ggcttgtcct tgccgttcca cagcgcgccg accacgagcg gccggttgag
     1261 gtcgccgtgc tcgaacatga cgaggacctc gtcgtccttg tcggggatga agaaccagcc
     1321 gcggttcttg ccggcgccga gcatgatgat cgggcaccag taggtggtgt cctgatcgga
     1381 caggatcggg atcttgagct tgacccggcc gagcttgctc gggtccttgt tgtcggtgac
     1441 gatgccgatc accggctcgt acacgcgaga cgcctcggtc tccgccgtac ggatctgggc
     1501 cgcgaacacg ttcgcgaggt cgtacatgaa tggctccgga tcttactacg gccggggggg
     1561 aagcgagctt cccggcgcgg tggtcgggat cgtgcctaac accgcgatac ctgggtcgct
     1621 ggcgagcctg tcgccggtcg gggtgtggat cttgccggcc gcgtcctcgg ccacgaggcc
     1681 caggccgatg gcgccctggc cggcgggaga ccagtcggga ggaatcgtga gcgggaagcg
     1741 ctcccggatg tagtcgccgg ccttccagcg ctcggtggag aacgcgccgc cggcggtggc
     1801 gcgcagggcc gtgcggagga tgcggctggg ggcgggggcg gcggtcgggt g
//
LOCUS       DASVOR010001596         2058 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395583,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001596 DASVOR010000000
VERSION     DASVOR010001596.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395583"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            744..>2058
                     /locus_tag="VF516_29970"
     CDS             744..>2058
                     /locus_tag="VF516_29970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112011.1"
                     /translation="MLTDPGRMLGKYQLIAEIARGGMGVVYLAMVQGPGGFNKLVVVK
                     ELKPELVEEPAFLTMFLDEARLAARLSHPNIVQTNEVGNDGDRYFMAMDYLDGRGLDQ
                     IRRRSKVAGFGLSVPMHLRVVCDMLAGLDYAHKLTDFDGSPLNIVHRDVSPQNVFVTF
                     DGQVKLLDFGIAKASDSMYETHAGVVKGKVSYMSPEQGRGWKVDARADVFSAGVMLWE
                     ALTGKRMREGKNEQEKLWALVSNEIPRASEIKPWVPPELDEICARAMAWNRDERYSSA
                     GAMQQDLERYLVSTGMNVTAREVGACVSELFREDRANTNSVIEAHIARLRGGTVREKL
                     PIIDVASRSVGSPTPSGERALTRPPFDPENLPSPDTPSARMGDDSAPMTSEPRRPVQP
                     RRDLRPVIITAVAGVVVGVVVIFLVARLTHDASPVTRAEPAPPTPQ"
BASE COUNT          337 a          726 c          702 g          293 t
ORIGIN      
        1 ggcgatcgcg cagcccggct ggctgccgct gcgcgcccgg ctcggcctcg gcgtccgctg
       61 agccaccggc gcgcagcgaa tgcatgatcg cggcgcccgc cgctgtgacc gttgcacgcc
      121 gcgcgctggt tcgcacacac atcgcgcacc gacgcaggtc gcgtgggtac gacccgtgga
      181 caggtcacgg aattctgacg ccagctcgcc cgctttgtgc ctcgagcgcc gccgccacgg
      241 ggaccctttg tgatgaacag cgtgttgcac aacaccaaat ttcccgaccg gctcgtctcc
      301 gtgagaccta ccatcgcggt gtttgtcgtc ggtaagtgca gcgccgtgcg gcagcaaata
      361 cgggcgatcc cgtgcgccga tcgacgcggt ttgaggtgat aatcgtgagc cggacgtgaa
      421 tcccggcgga cccgaaacac agcagtggcg cggagaccga ccgacgagtt agatcaggtg
      481 ttaccactcg atgagctgta catggagaac ccaggtgatg acggacgagg cgcgccgcgc
      541 gggctccgca ggggcgaccg tcatgccggt cgcgatggga cacctagccc cgaccggccc
      601 ggtccagatg atcggccaga aaccgcgcca tgacgaagga gagtagtcgc gacgtgacct
      661 ccagcgagcg ccgatcgggg ccggcccgga gccccaccaa aacttcttcc ggctctgcac
      721 cgccgggtag tacgctcccg tcgatgttga ccgacccggg gcgaatgctg gggaaatacc
      781 agctgatcgc agagatcgcg cgcggcggca tgggcgtggt ctacctcgcg atggtccagg
      841 gccccggcgg gttcaacaag ctcgtcgtgg tcaaggagct caagcccgag ctggtcgagg
      901 agccggcgtt cctgacgatg ttcctcgacg aggcccgcct ggccgcccgg ctgtcccacc
      961 ccaacatcgt ccagaccaac gaggtcggca acgacggcga tcgctacttc atggcgatgg
     1021 actacctcga cggccgcggc ctcgaccaga tccggcggcg ctccaaggtc gccgggttcg
     1081 ggctgtcggt gccgatgcac ctccgcgtgg tgtgcgacat gctcgccggc ctcgactacg
     1141 cgcacaagct gaccgacttc gacggctcgc cgctcaacat cgtccaccgc gatgtcagcc
     1201 cgcagaacgt gttcgtcacg ttcgacggcc aggtcaagct gctcgacttc ggcatcgcca
     1261 aggcgtcgga ctcgatgtac gagacccacg ccggcgtggt caagggcaag gtctcgtaca
     1321 tgtcccccga gcagggccgc ggctggaagg tcgacgcccg cgccgacgtg ttctccgccg
     1381 gcgtgatgct gtgggaggcg ctgaccggca agcggatgcg cgagggcaag aacgagcagg
     1441 agaagctgtg ggcgctggtc tcgaacgaga tcccgcgggc gtcggagatc aagccgtggg
     1501 tgccgcccga gctcgacgag atctgcgcgc gcgcgatggc gtggaaccgc gacgagcgct
     1561 actcgagcgc cggcgcgatg cagcaggacc tcgagcgcta cctggtctcg accggcatga
     1621 acgtgaccgc gcgcgaggtc ggcgcgtgcg tcagcgagct gttccgcgag gaccgcgcca
     1681 acaccaactc cgtgatcgag gcccacatcg cgcggctgcg cggcggcacg gtgcgcgaga
     1741 agctgccgat catcgacgtc gcgtcgcgct cggtcggcag cccgacgcca tcgggcgagc
     1801 gcgcgctgac ccgcccgccg ttcgatcccg agaacctccc ctcgccggac acgccgagcg
     1861 cgcggatggg cgacgactcc gccccgatga cgtccgagcc gcgccggccg gtccagccgc
     1921 gccgcgacct ccgcccggtg atcatcaccg cggtcgccgg cgtggtggtg ggcgtcgtcg
     1981 tgatcttcct ggtcgcgcgg ctgacccacg acgccagccc ggtgacccgc gccgaaccgg
     2041 ccccacccac cccgcagc
//
LOCUS       DASVOR010001597         4715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_542504,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001597 DASVOR010000000
VERSION     DASVOR010001597.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542504"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            307..1683
                     /locus_tag="VF516_29975"
     CDS             307..1683
                     /locus_tag="VF516_29975"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112012.1"
                     /translation="MAAQAEYSGTSLAQGAGVGDPRPADLWRGQRNRFLIEEARTTGT
                     WMHLVGGVIKLVLLLTFLDAGYPTWRALGAAGVFVVFALLQTAWIRSSHDAQSFDRVF
                     IAVNLSAQSMVTLIVGLTGGLRSPAVPDLLLPSVISLLFFGPHAVARWIAFASALLIV
                     AMGLLPESVVGPPLVFDHYAFALGVGLIWNLFLLHTMVSKMTRAASKAGHSMQCLREE
                     RLSEAEAQLRRLQSVGAKVAHELKNPLASIKGLCQLVARTPESERTQERLAVVASEIS
                     RMETILHEYLSFSRPLEDLRPESLDVSAIARDVLDVLAGRADQAGVVLTLDGAATSVH
                     GDSRRLKEALLNLVANAIEATPSGGSVALRLRNGGPAAVLEIRDTGRGIAPEDLERIG
                     TPFFTTRPSGTGLGVVLAQGVIQQHGGTLRYTSALGQGTTATITLPVKPLAAPEPAPR
                     AAMVEARA"
     gene            1680..3083
                     /locus_tag="VF516_29980"
     CDS             1680..3083
                     /locus_tag="VF516_29980"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8112013.1"
                     /translation="MSSVLLVDDEPGVLFTLSELLTERGHRVIESRSGDDALRKLDEA
                     DTVLTDLAMPGMDGLELVSHIVQRDPTLPVILLTAHGSERVAVAAMKQGAYDYLKKPF
                     DIDEVAVVIERALEARRLRIDNRRLSAEQTIGRRIIGDSRPMRRLLEATTRVASRDVT
                     VLVRGETGTGKEFVAELLHAQSARSKKPLIRFNCAALPAELADAELFGHVRGAFTGAS
                     SSRPGFFAQADGGTLILDEIGELPLAVQAKLLRVLQEGEIQPVGSGRIDKVDVRVVAS
                     TNRDLAAEAKTGAFREDLYYRLAVVELVVPPLRDRKDDIPALSAEFARRYGDRFGLGA
                     VSLEPALVDALVRSDWPGNVRQLENTIARLAALSAGGVIGLADYQLATGEGPPPLPPV
                     PIEAHDAGDEHSNAPAIDARGGPSLKEQVEAFERGLVARALDSTGGNQSEAARRLGVS
                     RVTLIDKMKKYGLGSKR"
     gene            3267..3539
                     /locus_tag="VF516_29985"
     CDS             3267..3539
                     /locus_tag="VF516_29985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112014.1"
                     /translation="MSKLRIAVITAAIALPLGAFAKDAMLKGHPNLEKARAALSDADR
                     YITASQKANEKVWGDEGGHGKKAKEAIATAKQELDLAAEWVNSHAK"
     gene            3640..4605
                     /locus_tag="VF516_29990"
     CDS             3640..4605
                     /locus_tag="VF516_29990"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEX8112015.1"
                     /translation="MESIVHVCNGDSTADSLSLADLPGEIRVWADALDQGPLLPVSDQ
                     EHWKLRDEFWASRGLRDAGGRLAAYDHGVDDAARAEELILWFEHDLFDQLALIRLLAR
                     LARRGLPAQLTIVSIDRHPEVPSFLGLGQLKPDQLAELWPRRTPLSRDAIDEAITAWI
                     AVTAPDPRALPFLTKRIKALPFLAGALERQLEELPDPTSGLSRTERQVLAAIARGEAT
                     AGALLGAVHAIDPRYPITDLVLLWTLHVLGRCGFIDGAPDGAPSPAALKSLRVTVTAR
                     GREALAGAIDRVHECGIDDWRGGVRLTGKGPVWRWDGAQRKLFEK"
BASE COUNT          651 a         1848 c         1574 g          642 t
ORIGIN      
        1 ggccggcggg ggccgcggcc ggccggggag ccggcggtct gggtgccggc ggcctgggtg
       61 ccggctgcgc aagcaccggc gccgcagcgc aaagcccaag gacgagcgtc attcgcgagg
      121 tcatatgcgg ccgaccaaag caccccccgt gccacggcgc cagaggccgc aaccatgacc
      181 ttccccgtcg ccgcgcgatc acggatgtcg gccgggctta cactctgcgg gtcacgaaac
      241 gtgacccgcg gcagtcacgt ttcgatcgca gccgcgcaac ccggcattca ggctagagtt
      301 gcctccatgg ccgcgcaggc agagtactct ggcacgtccc tggcacaagg tgcgggcgtg
      361 ggcgatccac gtcctgcgga cctctggcgc ggtcagcgca accgcttcct catcgaggag
      421 gcgcgcacga ccggcacgtg gatgcacctc gtcggcggcg tcatcaagct cgtcctcctg
      481 ctcaccttcc tcgacgccgg ctatcccacc tggcgcgcgc tgggtgcggc cggcgtgttc
      541 gtcgtgttcg cgctgctcca gaccgcctgg atccggtcgt cgcacgacgc ccagtcgttc
      601 gaccgcgtgt tcatcgccgt caacctgtcg gcgcagtcca tggtgacgct gatcgtcggg
      661 ctgaccggcg ggctgcgctc gccggcggtg cccgacctgc tcttgccgtc ggtgatctcg
      721 cttttgttct tcggccccca cgcggtcgcg cgctggatcg cgttcgccag cgcgctgctg
      781 atcgtggcga tgggccttct gcccgagtcc gtggtcggcc cgccgctggt gttcgatcat
      841 tatgcgttcg cgctcggcgt cggcctgatc tggaacctgt tcctgctcca caccatggtc
      901 tccaagatga cgcgcgcggc gagcaaggcc ggtcactcca tgcagtgcct gcgcgaggag
      961 cggctgtccg aggccgaggc ccagctccgc cggctgcagt cggtcggcgc caaggtcgcc
     1021 cacgagctca agaacccgct cgcgtcgatc aagggcctgt gccagctggt cgcgcgcact
     1081 cccgagagcg agcgcaccca ggagcggctc gcggtcgtcg cctccgagat ctcgcggatg
     1141 gagaccatcc tccacgagta cctgtcgttc tcgcggccgc tcgaggacct gcgccccgag
     1201 tcgctcgacg tcagcgcgat cgcgcgcgac gtgctcgacg tgctcgccgg gcgcgccgac
     1261 caggccggcg tcgtgctgac gctcgacggt gccgccacct cggtgcacgg cgacagccgc
     1321 cggctcaagg aggcgctgct caacctggtc gccaacgcga tcgaggccac cccgagcggc
     1381 ggctccgtcg cgctccgcct gcgcaatggc gggcccgcgg cagtgctcga gatccgggac
     1441 accggccggg gcatcgcgcc cgaggacctc gagcggatcg gcacgccgtt cttcaccacc
     1501 cggccgagcg gcaccggcct gggcgtcgtg ctcgcgcagg gcgtgatcca gcagcacggc
     1561 ggcacgctgc gctacaccag cgcgctgggc cagggcacga ccgcgacgat cacgctgccg
     1621 gtcaagccgc tggccgcccc cgagcccgcc ccgcgcgccg cgatggtcga ggcccgcgcg
     1681 tgagctcggt cctcctggtc gacgacgagc ccggggtcct gttcacgctg agcgagctgc
     1741 tcaccgagcg cggccaccgc gtgatcgaga gccggtcggg cgacgacgcc ctgcgcaagc
     1801 tcgacgaggc cgacaccgtg ctcaccgatc tggcgatgcc ggggatggac ggcctcgagc
     1861 tggtcagcca catcgtgcag cgcgatccga cgctgcccgt catcttgctc accgcccacg
     1921 gcagcgaacg cgtcgcggtc gccgcgatga agcagggcgc ctacgactac ctcaagaagc
     1981 cgttcgacat cgacgaggtc gccgtcgtca tcgagcgcgc gctggaggcc cgccggctgc
     2041 ggatcgacaa ccgccggctg tccgccgagc agacgatcgg ccgccggatc atcggcgaca
     2101 gccggccgat gcgccggctg ctcgaggcca ccacccgggt cgccagccgc gacgtcaccg
     2161 tgctggtccg cggcgagacc ggcaccggca aggagttcgt cgccgagctg ctccacgccc
     2221 agtcggcgcg cagcaagaag ccgctgatcc ggttcaactg cgccgccctg cccgccgagc
     2281 tcgccgacgc cgagctgttc ggccacgtcc gcggcgcgtt caccggcgcg agctcgagcc
     2341 gccccgggtt cttcgcccag gccgacggcg gcacgctgat cctcgacgag atcggcgagc
     2401 tgccgctcgc ggtccaggcc aagctcctgc gcgtgctcca ggagggcgag atccagccgg
     2461 tcggctcggg ccggatcgac aaggtcgacg tccgggtcgt cgcctcgacc aaccgcgacc
     2521 tcgccgccga ggccaagacc ggcgcgttcc gcgaggacct ctactaccgg ctcgccgtcg
     2581 tcgagctcgt cgtcccgccg ctgcgcgacc gcaaggacga catccccgcg ctctccgccg
     2641 agttcgcccg ccgctacggc gaccgcttcg gcctgggcgc ggtcagcctc gagcccgccc
     2701 tcgtcgacgc cctggtcagg tccgactggc ccggcaacgt ccgccagctc gagaacacga
     2761 tcgcccggct cgccgccctg tccgccggcg gcgtgatcgg cctggccgac taccagctcg
     2821 ccaccggcga gggcccgccg cccctgccgc ccgtcccgat cgaggcccac gacgccggcg
     2881 acgagcacag caacgccccc gcgatcgacg cgcgcggcgg cccgtcgctc aaggagcagg
     2941 tcgaggcgtt cgagcgcggc ctggtcgccc gcgcgctcga ctccaccggc ggcaaccagt
     3001 ccgaggccgc ccgccggctc ggcgtctcgc gcgtcacgct gatcgacaag atgaagaagt
     3061 acggcctggg ctccaagcgc tgaccccgcg tgcgtgcctt gcccgcaccg tccgccccgg
     3121 gtgtgaattt cataccgcgc ggacacaatg cccgaccgag gcagaacgcc atggccggat
     3181 cgggaataag ccggcggaat caggccgtgc gcccgggcac ggccggtgca cgacgctcgc
     3241 ctcacttctc ttccacagga gaccccatgt ccaagcttcg cattgccgtg atcaccgccg
     3301 ccatcgccct cccgctcggc gcgttcgcca aggacgccat gctcaagggc cacccgaacc
     3361 tcgagaaggc ccgcgcggcg ctgagcgacg ccgacaggta catcaccgcg tcgcagaagg
     3421 ccaacgagaa ggtctggggc gacgagggcg gccacggcaa gaaggccaag gaagcgatcg
     3481 cgaccgccaa gcaggagctc gatctcgcgg ccgagtgggt caactcgcac gccaagtgat
     3541 cggcgcgccc ccgagcgcac gcgcggcggg cccgcaaagc tcgtccaaac gctctccggc
     3601 gcttgcgagc acgcggcgcc ggctgctacg cccgggggta tggagtcgat cgtccacgtc
     3661 tgcaacggcg acagcaccgc cgattcgctc tcgctcgctg acctccccgg tgagatccgc
     3721 gtgtgggccg atgccctcga tcagggcccg ctgctgccgg tctccgatca ggagcactgg
     3781 aagctgcgcg acgagttctg ggcctcgcgc ggcctgcgcg acgcgggcgg ccggctcgcc
     3841 gcctacgacc acggcgtcga cgatgccgcc cgcgccgagg agctcatcct gtggttcgag
     3901 cacgacctgt tcgatcagct cgccctgatc cgcctgctcg cccgcctcgc ccgccgcggc
     3961 ctccccgcgc agctgaccat cgtctcgatc gaccgccacc ccgaggtccc cagcttcctc
     4021 ggcctgggcc agctcaagcc cgaccagctc gccgagctat ggccgcgccg caccccgctg
     4081 tcccgcgacg cgatcgacga ggccatcacc gcctggatcg ccgtcaccgc ccccgatccg
     4141 cgcgccctgc cgttcctcac caagcggatc aaggccttgc cgttcctcgc cggcgccctc
     4201 gagcgccagc tcgaggagct gcccgatccg accagcggcc tgtcgcgcac cgagcgccag
     4261 gtcctggccg ccatcgcgcg cggcgaggcc accgccggcg ccctgctcgg cgccgtccac
     4321 gcgatcgatc cgcgctaccc gatcaccgac ctggtcctgc tctggaccct ccacgtcctc
     4381 ggccgctgcg gcttcatcga cggcgccccc gacggcgccc ccagcccggc cgcgctcaag
     4441 tcgctgcgcg tcaccgtcac cgcgcgcggc cgcgaggccc tcgccggcgc gatcgaccgc
     4501 gtccacgagt gcggcatcga cgactggcgc ggcggcgtcc gcctcaccgg caagggcccg
     4561 gtctggcgct gggacggcgc gcagcgcaag ctgttcgaga agtgatcgtc aagtgcgcac
     4621 tgctcgccgg cctgctcgct gcctgcggcc cctggggcga tcgcatcggg cgctccgagc
     4681 agcccactgc gatggacgcg gcgaagtacc gggtc
//
LOCUS       DASVOR010001598         3395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_221087,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001598 DASVOR010000000
VERSION     DASVOR010001598.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3395
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221087"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            178..471
                     /locus_tag="VF516_29995"
     CDS             178..471
                     /locus_tag="VF516_29995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HEX8112016.1"
                     /translation="MQSDTFGGKAIMASLACCARCGSDDLEDKEVEKLIRGGDDVAAL
                     RGPATVCHHCGERYPPVEAIRALEQARRDLAEGKLARFRTVGRVVVPQLDPSG"
     gene            complement(829..1599)
                     /locus_tag="VF516_30000"
     CDS             complement(829..1599)
                     /locus_tag="VF516_30000"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8112017.1"
                     /translation="MRIEAAAVARTKRRSEPGQLVLFKQRGGKRRGAGRPPKGTRAGS
                     PHKERPFLHARYPVHIVLRVVGAVGNLRRRCVYQAIREATLTTALREDFRIVQLSIQR
                     THVHLLVEANDKAALARGMQGFQISAAKHLNAAISKGRPGPRRRGVVFPDRYHAEIIT
                     SPRQARHALSYVMNNWRKHREDLRAPMLGWAVDWFSSAAMFTGWAEYGDEPFLWRGPA
                     TYDPLVVYQPRTWLLREGWKKSGSISCHEVPSDKHQPR"
     gene            2288..>3395
                     /locus_tag="VF516_30005"
     CDS             2288..>3395
                     /locus_tag="VF516_30005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112018.1"
                     /translation="MSRQRGASSMHRAEQVAQDLIARRIPARAGELLEIIHAINPTGR
                     SLSAADERRRYELKARLQSLLIRNFAEDLAVTAEAPGVVSIRHRYLGQDACHARIDEL
                     DDDARARVRWMLDVGEVNEPHGGMVTPPAARAADDADLLDQGRRALAEFDYETARAHF
                     ARAMQHSNGDPAAARALLELLVDHMAADGDALALEDQLAPRAAADAEVRALLAVAAAR
                     MGNAAAANRLLEGLVGPRVTEAWKALAEDAARRRACDELDHRIARLTEADPTHPDLVR
                     FREQAQRLRADARRPAEQELVQLAEAGDDEAAEARARALLARWPDSRVAGRILGGIQD
                     RRRASEAERLLASARSALLNGDTGLATELCRQAGN"
BASE COUNT          496 a         1189 c         1225 g          485 t
ORIGIN      
        1 acgccgccga gctcgatgag ccgccgggtc cgccgcgccg gaggcctcga ccccgactcc
       61 gccgcccggg tcgacgtcgt gctgcgcgcg atgcacgcgg ccggcgcgat cgacgccgcc
      121 gagctcgagg ccgcccgcac cgcccccctg cggttcaccc cctccgcggt ggattgagtg
      181 cagagtgaca ccttcggagg gaaagccatc atggcaagcc ttgcctgctg cgcgcgctgc
      241 ggctccgatg acctcgagga caaggaggtc gagaagctga tccgcggcgg cgacgacgtg
      301 gccgcgctgc gggggcccgc cacggtctgc caccactgcg gcgagcgcta cccgccggtg
      361 gaagcgatcc gcgcgctgga gcaggctcgc cgggaccttg ccgaaggcaa gctggcgcgc
      421 tttcggacgg tcggccgcgt cgtggtgccg cagctggacc cgagcggctg agcgaggccc
      481 tgggaagtgc ggcgcaagtg cgcccggttc gcacacagcg cggcgagcac cgcggttcgg
      541 tgtcgcgagc tgagcggcgc gaagggggcg cggaggttgg agtcggtgac ggcgacgtcg
      601 cccgcgtcgg cgagcatgaa cgggacgagg gtgcggagct cgtcatccac gcggcggccg
      661 tgcaggacga tccggcggac gagatccgtc tcgctggtgc gcgactctct tgaccgcgtc
      721 ggtgaggtgt ctgcgctcgg cggcgagccg gacgacgtcg ccgcggtcgg cgcctgcccg
      781 ccgccccacc agccggtgcc gttccggatc tgcaacctcg cgtacggcct acctcggctg
      841 gtgcttgtcc gacgggacct cgtggcagga gatcgatccg gatttcttcc agccctcacg
      901 gagcagccac gtcctgggct ggtacacgac cagcggatcg tacgtcgccg ggccgcgcca
      961 caggaacggc tcgtcgccgt actcggccca gccggtgaac atcgccgcgc tggagaacca
     1021 gtcgaccgcc cagcccaaca tcggcgcgcg aaggtcctcc cggtgctttc tccagttgtt
     1081 catcacgtag ctcagcgcat gccgcgcctg gcgcggcgag gtgatgatct cggcgtggta
     1141 ccggtcgggg aacaccacgc cccgccggcg tggcccgggt ctgcccttgc tgatcgccgc
     1201 gttgaggtgc ttggccgccg agatctggaa cccttgcatc cccctggcca gcgcggcctt
     1261 gtcgttggcc tccaccagca ggtggacgtg cgtccgctgg atgctgagct gaacgatgcg
     1321 gaagtcctcg cgcagcgccg tggtgagcgt ggcctcgcgg atcgcctggt atacgcagcg
     1381 ccggcgcagg ttgccgacgg cgccgaccac gcgcaggacg atgtggaccg gatacctcgc
     1441 gtggaggaac ggccgttcct tgtgcggcga gccggcccgg gtgcccttcg gcgggcggcc
     1501 ggccccgcgc cgcttgccgc cgcgctgctt gaacagcacc agctgtccag gctcggagcg
     1561 ccgcttcgtg cgggcgacag ccgccgcctc tatcctcatg gccctcagaa ccctcggcca
     1621 attgaattcg ccgtaatttg ttgtaacgag atgtaaccaa ctgatgtaac caactatcga
     1681 catcgtcgtg ccaacccccg gtgcatgttg cgacccaagg tcgagcggcc gctgctggag
     1741 tttggcggat ggcgatcgag gatcgatgca cggcagctgc agccgggacc ccgggagacc
     1801 gcgtggacgg ccctggtgac tggctcgtcg aagttgacca ggatgaccag gacgacccac
     1861 tcacagtgca tcgccccgcg cgtacgctcc attccattca tttcattgat tcccgatctc
     1921 cgaggaatgg gagagcacag ccatcgaaca gcggggttgc agaggattcc cggatggagc
     1981 tcctcgagga cgggactggc gcgtgcgccg cgccggcgaa tcgaatcgtg agagcctccg
     2041 cttcggtgct gcagcgctgc cacgacgggc caaagccgcc tcgcgccacg ccggcctgtt
     2101 cgccgcccgc gcggtagagc ggtagatccg atcggtcgct gtgtcgttac gcgcgggctc
     2161 agaaggcgag aatcccgcac ccgccgccgc gcgctgctgt cgagtccatg tggtcgcgtc
     2221 caccggtcga tgcggcgctc ctgctgcagg cttgcacgat cagggcggct ctggcaccct
     2281 gcgctccatg agccggcagc gcggtgcgtc gagcatgcat cgcgccgagc aagtcgcgca
     2341 ggacctgatc gctcggcgca ttccagcccg agccggcgag ctgctcgaga tcatccatgc
     2401 gatcaacccg accggccgct cgctcagcgc agcagacgag cgccgccgct acgagctcaa
     2461 ggcgcgcctc cagagcttgc tgatccgcaa cttcgccgag gatctcgctg tgacggccga
     2521 ggcaccgggc gtggtctcga tccgtcaccg ctacctggga caggatgcgt gccatgcgcg
     2581 gatcgacgag ttggacgacg atgcgcgcgc ccgcgtgcga tggatgctgg acgtcggcga
     2641 ggtcaacgag ccgcacgggg gcatggttac accgccggcg gcccgggcgg ccgacgacgc
     2701 cgacctgctc gaccaggggc gccgcgcgct ggccgagttc gactacgaga cggcgcgggc
     2761 tcacttcgcg cgcgcgatgc agcactcgaa cggagatccg gcggcggccc gcgcgctgct
     2821 cgagctgctg gtcgaccaca tggcagcgga cggtgacgcg ctggcgctcg aggatcagct
     2881 ggcgccgcgc gccgcggccg acgccgaggt tcgcgcactg cttgcggtcg ccgccgcgcg
     2941 catggggaac gcagcggcgg cgaatcgcct gctggagggc ctggtgggtc cccgggtcac
     3001 ggaggcatgg aaggccctcg cggaggatgc ggccaggcgc cgggcgtgcg acgagctcga
     3061 ccaccgcatc gctcgtctga ccgaggccga cccgacgcac cccgatctgg ttcggttccg
     3121 agagcaagcg cagcggctgc gcgccgatgc ccggaggcca gccgagcagg agctggtgca
     3181 gctggccgaa gccggtgacg acgaggccgc cgaagcgcgc gcgcgagcgc tgctcgcccg
     3241 ctggcccgac agccgcgtcg ccggccggat cctgggcggg atccaggacc ggaggcgcgc
     3301 cagcgaggcc gagcgactgc tcgcgagcgc gcgctcggcg ctcttgaacg gcgacaccgg
     3361 gctcgcgacc gagctgtgcc ggcaggcggg caacg
//
LOCUS       DASVOR010001599         5267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_192027,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001599 DASVOR010000000
VERSION     DASVOR010001599.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192027"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1332)
                     /locus_tag="VF516_30010"
     CDS             complement(<1..1332)
                     /locus_tag="VF516_30010"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEX8112019.1"
                     /translation="MSQSTPTANEPIAIVGMSARFPGAPDLDAFWRLVRDGVDAIREV
                     PAARFDVDPFHDPAPGTPGKLSSRWGGFLDDIDRFDAAFFGISPREAARMDPQQRLAL
                     EAAWEALDDGGQRPLDLRGTATGVFLGVHSDEYFRLAARDAAEIDLLSVAGGARSAVA
                     GRISYVLGLEGPSLVFDTDRSSSLVAVRAACESLRRGECSLALAGGVNLILAPEPGLG
                     FARANLLAADGRIKFCDARADGIVRSDGVGMVALKPWSAARRDGDRVHALILGAAINH
                     DGGRSGDLMTPSSASQAALIRAACRDAGVSPHDVQYLEAHGTGTIVGDPVEVAAAAAV
                     LQHGRDATRPLRIGSVKTNIGHAEAAAGIAGLIKVALAIEHRLLPASLHFATPSPRIA
                     FEGVAVQAATTPWPDERRPLVGGVTSLGLTGVNAHVIVGEAPRDEAPAAAPG"
     gene            1620..1760
                     /locus_tag="VF516_30015"
     CDS             1620..1760
                     /locus_tag="VF516_30015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112020.1"
                     /translation="MTLTPTGVVRAGFACDTTADEVDRWLDGVRGSRAGPRGVRDTRR
                     WP"
     gene            1785..2039
                     /locus_tag="VF516_30020"
     CDS             1785..2039
                     /locus_tag="VF516_30020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112021.1"
                     /translation="MISAWSHGHEPGALHRPQLLDRARRASSRTQASSMRRPTRCTPI
                     GRPSWLAVPSIGAAAAQDRRDWRYDVIERLTTDKDGLSLK"
     gene            2465..3919
                     /locus_tag="VF516_30025"
     CDS             2465..3919
                     /locus_tag="VF516_30025"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112022.1"
                     /translation="MLSLEIMAAAIILGAIMLFWDRKRELAALAGLDGGGGLAVVWGR
                     RRIGKTRLLLEWAERAGGVYTVADQSAPHLQRDYFARAISRALPGFSDVTYPDWERLL
                     SRLATDAMAAGFTGPIVIDELPYLVAGSPELPSILQRWVDHDVRRARLRVALAGSSQR
                     MMQGLVLSHAAPLYGRARVTLDLAGLPPEHLYEAFGRLTPAAVVEHWAGFGGVPRYWE
                     LAAERRGSARDRVTALALDPLGPLFAEPDRLLLEESPPAIELRPVLDAIGGGAHRLSE
                     IASRLGRPATSLGHALDRLLGMTLVRREVPYGESAKRSKRSLYKIDDPFLRMWFRVVA
                     PNRAALTAGTPASRAAVLDEHWHLLLGQAWEDLCARGVTMLHGALARRGRWQPPRRYW
                     HGAEPEWDLIADSLDGERVLAGEAWFSARPATVAVLTREVERLARRPLPAALGNREVV
                     RALFVPSVARPKPKIIGSVHIVTLDDFLTRVRPR"
     gene            complement(3935..>5267)
                     /locus_tag="VF516_30030"
     CDS             complement(3935..>5267)
                     /locus_tag="VF516_30030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8112023.1"
                     /translation="PGTRRVLPAGVVDLEAIDPDAVALPAWYRPDPGRARDLAMVFLS
                     TGRDAPLRAMRITNRRWAFSALGAAAAATLTTRDTVYCCLPLHHPSGTLVATGSALIS
                     GARLALASAFTPDPWAEPFWDEVRRYGATVVYYAGEMCRRLVDAPPVLGENNNPVRLF
                     AGSGLRAGVWRRLVDRFGPVAVLEMYASTEAHIVLANARGKKLGSVGRPLPGSPDVAI
                     AAWSLADDDFVRDGAGRLIHARLDEPGMLVAALGAPSSTAGADLAHIDPRRIVGDAFA
                     PGDRWFVTGDLFRVDAEGDHWFVDHRRHMIATHHGPVASTRIEDAIYDVPGVALCAVV
                     GRPDPDPGTDPGTGPAPAQFPVAAIQLHSGPGPGAALDLDALSRAVAALPEYARPRRI
                     RRVDALPMTDGYRPIKQALAGLDLSDGPEVIAWDPRAQRYQPTVEPAARSA"
BASE COUNT          734 a         1871 c         1935 g          727 t
ORIGIN      
        1 ccccggcgcc gccgccggcg cctcgtcgcg cggcgcctcg ccgacgatca catgggcgtt
       61 cacgccggtc aggccgagcg aggtcacgcc gccgaccagc ggccggcgct cgtcgggcca
      121 cggcgtggtc gctgcctgga ccgcgacgcc ctcgaacgcg atccgagggc tgggcgtggc
      181 gaagtgcaag ctcgccggca gcaggcgatg ctcgatcgcc agcgcgacct tgatgagccc
      241 ggcgatcccc gccgcggcct cggcgtgccc gatgttggtc ttgaccgagc cgatccgcag
      301 cgggcgggtc gcgtcgcggc cgtgctgcag caccgcggcc gcggccgcga cctcgaccgg
      361 gtcgccgacg atcgttccgg tgccgtgggc ctcgaggtac tggacgtcgt ggggggacac
      421 gccggcgtcg cggcaggccg cgcggatcag cgcggcctgg ctggcgctcg agggcgtcat
      481 cagatcgccg ctccgcccgc catcgtggtt gatcgccgcg ccgaggatca gcgcgtggac
      541 ccgatcaccg tcgcgccgcg cagcgctcca cggcttgagc gcgaccatgc ccacgccgtc
      601 gctccggacg atcccgtcgg cgcgcgcgtc gcagaacttg atccgcccgt ccgccgccag
      661 caggttcgcc cgcgcgaacc cgaggcccgg ctccggcgcg aggatcaggt tgacgccgcc
      721 cgccagcgcg agcgagcact ccccgcggcg caggctctcg cacgccgcgc gcaccgcgac
      781 cagcgacgac gaacgatcgg tgtcgaatac caggctcggg ccctcgagac cgagcacgta
      841 cgagatccgt cccgccacgg cgctgcgcgc gccacccgcc accgacagca gatcgatctc
      901 ggccgcgtcg cgcgccgcca gccggaagta ctcgtcgctg tggaccccca gaaacacgcc
      961 ggtcgccgtg ccgcggagat ccagcggccg ctggccgccg tcgtcgagcg cctcccacgc
     1021 cgcctcgagc gcgaggcgct gctgcggatc catccgggcg gcctcgcgcg gtgagatccc
     1081 gaagaacgcg gcgtcgaatc gatcgatgtc gtcgaggaaa ccaccccagc gcgacgacag
     1141 cttgcccggc gtccccggcg ccggatcatg gaacgggtcg acgtcgaacc gggcggcagg
     1201 cacctcgcgg atcgcgtcga caccgtcccg gacgagccgc cagaatgcgt ccagatcggg
     1261 cgcacccggg aagcgtgcac tcatccctac gatcgcgatc ggctcgttgg ctgttggcgt
     1321 gctctgcgac aagcaatttg agccagtacg acgttgagcg gcgtactatc ttgcggaatg
     1381 cctcgacgat caagagcctt ttaacgagct tgagccactc ccgtgcacat caatggaatt
     1441 gatgatcatt cgccgcggtc gaccgactcg aaggtgctcg cgcggcccga gcggctcggc
     1501 ctcggcatcg aactggacgt atggatggcc agctcaccgc ggcccgggac cgccatcgcg
     1561 aagcacacgc gcgaatccct gcgcgaggtc ttgcgatctg ctgcgcggga tcacatcgga
     1621 tgaccctgac gccgaccggc gtcgtgcgcg ccggcttcgc ctgcgacacg acggcggacg
     1681 aggtcgatcg ctggctcgac ggggtccggg gctcccgcgc cggcccgcgt ggggtgcgcg
     1741 acacgcggcg ctggccgtga accacaccaa tcgcgatcgc ggccgtgatc tccgcctggt
     1801 cacacgggca cgagccaggc gcgctgcacc ggccgcagct gctcgatcga gccaggcgcg
     1861 ccagctcgcg cacgcaggca tcatcgatgc gccgtccgac gcgctgcacg ccgatcggcc
     1921 gtccgtcatg gctcgccgtc ccgtcgatcg gcgccgcggc ggcccaggat cggcgagact
     1981 ggcgatacga cgtgatcgaa cgcctgacga cagataaaga tggattatcc ttgaaatagc
     2041 ggaaagctcg agattcggag acatcatgcg tacgcgattg aattgactga tatgagaaat
     2101 caatttggct ccagggaatc gccgcgagat cgaacgacac cttctgccaa ggttcgtgcg
     2161 cgcccccggt ggccttggcc tcgtcgcccg atcccgccac gccgcgcccg ggcgtcggcg
     2221 catgacggac cacgccgggc ctcatcgccg accggcaccg agcgatcagg acgatagaag
     2281 atcggatgga cgctcgcatg tccatctgac ccgcggcgat tgaaccccgt gttgcgcaca
     2341 tgttgcggac tgcgtcggcc gcaccacgca gtcggtcgat gcccgcgccg attccgctcg
     2401 cctcgccctg gttgcgaccc ggaccccgac cggtcggcgg tgttcgccgg gccggcgacg
     2461 cggcgtgctt tcccttgaaa taatggcggc agcaattatt cttggcgcca tcatgttgtt
     2521 ctgggaccgc aaacgcgagc tggcggcgct cgccgggctc gacggcggtg gcggcctggc
     2581 cgtggtctgg ggccggcgcc ggatcggcaa gacccgcctg ttgctggagt gggccgagcg
     2641 cgccggcggg gtctacaccg tggccgacca gtcggcgccc cacctgcagc gggactactt
     2701 cgcccgtgcg atctcgcggg cgctgccggg cttctcggat gtgacctacc cggactggga
     2761 gcggctgctg tcccggctcg cgacggatgc catggccgcg gggttcaccg gtccgatcgt
     2821 gatcgacgag ctgccctacc tggtggccgg atcgccagag ctgcccagca tcctgcagcg
     2881 ctgggtcgat cacgacgtcc ggcgcgcccg gctgcgcgtg gcgctcgccg gttccagtca
     2941 gcgcatgatg caagggctgg tgctttcgca cgcggctccg ctctacggtc gggctcgcgt
     3001 gaccctcgat ctggccggcc tgcccccgga gcacctgtac gaggcgttcg ggcggctgac
     3061 cccggccgcc gtggtcgagc actgggcggg cttcggcgga gtaccgcgtt actgggagct
     3121 cgcggcggag cgccgggggt cggcgcgcga tcgggtgacc gcccttgcgc tcgacccgct
     3181 ggggccgctg ttcgcggagc ccgatcggct gctcctcgag gagagcccgc ctgcgatcga
     3241 gctccgcccc gtgctggatg caatcggcgg gggagcgcat cgcctttcgg agatcgccag
     3301 tcgactcggt cgcccggcta cctcgctggg acatgcgctc gatcggttgc ttggaatgac
     3361 cctggtgcgg cgcgaggtgc cgtacggcga gtccgccaag cgcagcaagc gcagcctgta
     3421 caagatcgac gatccgttcc tccgcatgtg gttccgtgtg gtggcgccca atcgagccgc
     3481 gctgaccgcc ggcacgccgg cctcgcgcgc cgcggtcctc gacgaacact ggcacctctt
     3541 gcttggccaa gcctgggagg acctgtgtgc gcgcggcgtg acgatgctcc acggagccct
     3601 ggcccgacgc gggcggtggc agccgccgcg tcgctactgg cacggcgcag aacccgagtg
     3661 ggacctcatc gctgactcgc tcgacggtga acgagtgctg gccggtgagg cctggttctc
     3721 ggcccgcccg gcgaccgtcg cggtgctgac ccgcgaagtg gagcggcttg cgagacgtcc
     3781 gctgcccgcg gcgctcggga accgcgaggt cgtacgagcg ctgttcgtcc cgtcggtcgc
     3841 aaggccgaag ccgaagatca tcggctcggt gcacatcgtc acgctcgacg acttcctgac
     3901 ccgcgtacgg ccccgctgac atccggcgcc ctcgtcacgc cgagcgggcg gcgggctcga
     3961 cggtgggctg gtagcgctgg gcgcgcgggt cccaggcgat gacctcgggg ccgtcggaca
     4021 gatcgaggcc cgcgagcgcc tgcttgatcg ggcggtagcc gtcggtcatc ggcagcgcat
     4081 cgacccggcg gatccggcgc ggccgggcgt actcggggag cgcggcgacc gcgcgcgaca
     4141 gcgcgtcgag gtcgagcgcg gcaccggggc cggggccgga atggagctgg atcgccgcca
     4201 cggggaactg cgcgggcgcg gggccggtgc cggggtccgt gccggggtcc ggatcagggc
     4261 gaccgacgac ggcgcacagc gcgacgccgg ggacgtcgta gatcgcgtcc tcgatccggg
     4321 tcgatgcgac cgggccgtgg tgagtggcga tcatgtggcg gcggtgatcg acgaaccagt
     4381 ggtcgccctc ggcgtcgacc cggaacaggt cgccggtgac gaaccagcgg tcgccgggcg
     4441 cgaaggcgtc gccgacgatc cggcgcggat cgatgtgcgc gaggtcggcg ccggcggtgg
     4501 acgacggggc gccgagcgcg gcgaccagca tgccgggctc gtcgagccgg gcgtggatca
     4561 ggcggccggc gccgtcgcga acgaagtcgt cgtcggcgag cgaccacgcg gcgatcgcga
     4621 cgtcggggct gccgggcagc ggccggccga ccgagccgag cttcttgccg cgcgcgttgg
     4681 cgagcacgat gtgggcctcg gtcgaggcgt acatctcgag cacggcgacc ggaccgaacc
     4741 gatcgaccag ccggcgccac acgccggcgc gcaggccgct gccggcgaac agccgcaccg
     4801 ggttgttgtt ctcgccgagc acgggcggcg cgtcgaccag ccggcggcac atctcgccgg
     4861 cgtagtagac gacggtggcg ccgtagcggc ggacctcgtc ccagaatggt tcggcccatg
     4921 ggtcgggcgt gaacgcgctg gcgagcgcga gccgggcgcc gctgatcagc gcgctgccgg
     4981 tcgcgaccag ggtgcccgac gggtgatgca gcggcaggca gcagtacacg gtgtcgcgcg
     5041 tggtcagcgt ggcggcggcc gccgcgccca gcgccgagaa cgcccagcgc cggttggtga
     5101 tccgcatcgc gcgcagcggc gcgtcgcgcc cggtcgacag gaacaccatc gcgaggtcgc
     5161 gcgcgcggcc ggggtcgggg cggtaccagg cgggcagcgc gacggcgtcc ggatcgatcg
     5221 cctcgagatc gacgacgccg gcggggagca cgcggcgcgt gccgggc
//
LOCUS       DASVOR010001600         4132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_250531,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001600 DASVOR010000000
VERSION     DASVOR010001600.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250531"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..355)
                     /gene="cutA"
                     /locus_tag="VF516_30035"
     CDS             complement(26..355)
                     /gene="cutA"
                     /locus_tag="VF516_30035"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEX8112024.1"
                     /translation="MTDAAALVVFSTFPDADTAARIARALVEEQLAACVNVVPAVRSI
                     YRWEGNVCDEAETLAVIKTTAERYAALAARITELHPYQVPEVIALPLADGHPPYLAWL
                     ADQVARP"
     gene            400..783
                     /locus_tag="VF516_30040"
     CDS             400..783
                     /locus_tag="VF516_30040"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112025.1"
                     /translation="MLIVRKYGGTSVGSIERMRNAAARCLATARQGHQVAVVVSAMSG
                     ETNRLLKLAGEIHEDPNDREVDVIVSTGEQVSVGLVALTIRAAGGKGGARAAAAPVRR
                     VGYSAGGWAAPTARARPAATTARGR"
     gene            726..1472
                     /locus_tag="VF516_30045"
     CDS             726..1472
                     /locus_tag="VF516_30045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112026.1"
                     /translation="MGCTNCKSKTGCDHRKGEMIASVDRTLAQLYPTRTWGEPDDRQL
                     AGPAPDELAALADELAGELHAATFVRPGRDDEPCDYLYVLCMGRSPCAIQVRDHGVPV
                     PAEWASAEAGSAAIHELYLRLVVSQRARIAAVQQVAVEVVPAGRGYLVIERPRAGVYD
                     APLLPRMQKLVAILPAYELLHVDFGEIAHAPPGYHAGAWRELFGGEPAIANYLFYPQP
                     TTMIATGFIDAEGIAAEVIAAEVSSRMIRR"
     gene            1469..2164
                     /locus_tag="VF516_30050"
     CDS             1469..2164
                     /locus_tag="VF516_30050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112027.1"
                     /translation="MSDAEIADEVRAVLRELVDEIGAISARILEHDELRTGVPARTLA
                     LGGGEYLRVELPTRHGREVEVEAAFDRTVRQLRAIRRRWEIARLPEISIAPGAQPPSG
                     RVIERIHGYLRALASIDRASNALVTRGNQLIAAARPPDELEAARWPFLARRALASHAP
                     HSSHGEVVDPDAYAMSFWYSAVLIVLLAEPYALDFVRYRSRQVARELAHLLPLLEPDP
                     DTPAAIGRPPTGP"
     gene            complement(2190..3500)
                     /locus_tag="VF516_30055"
     CDS             complement(2190..3500)
                     /locus_tag="VF516_30055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112028.1"
                     /translation="MTTTRFALAIALAGCGRVGFSEIAGPSGSDAGADSATAADGATG
                     DTVTLVVTSDEYLNEPAGLPIAGATVLVERSTGTDRMVTDATGRAQFAAAGVIACHVI
                     YQSTIGWRGYTVQPPPAGTIELGSRPAFNQNHGMTFALPSNATASDYTVRLPEHCASP
                     PYSGSPSVQFNYAPACEGATIHAVGFVRTQVGSGLPDLYLDAGMVTLRNGATQQVTGS
                     YQQLPTRTLQLTDLPSGATSVAAGVVARAGLDLTPLSPSIDSFTPSGGAVTVQEEVAP
                     GGNTLGIGASGGLPPQYITSSELLAPLSSATAATFSAKDMLPLFESMTITDPTQISWT
                     GAAGGTITIVEHVGGDFQWDWYLPPSATVARLPAIPPDLGVPAPQVPPDASVTKLAVP
                     GAAGGDLVPTIDRRWPAWPHDALLLPAAGGAWTQILYSAAIGPP"
     gene            complement(3565..>4132)
                     /locus_tag="VF516_30060"
     CDS             complement(3565..>4132)
                     /locus_tag="VF516_30060"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8112029.1"
                     /translation="MGPLATAQQRDDVRAGIARLAAETEAIFGGTGEVPAPGPIGAPP
                     GKGFFVGPVVRATQSPLTCAALHDQEVFGPVITLGAYPGDPGAAAAFVARGDGCLVSA
                     AYSDDRDWVGRFIGSAAPWAGRLYLGSSKMASQSPGPGTALPALLHGGPGRAGGGEEL
                     GGERGLRLYLQRCALEGDASVLKALLGE"
BASE COUNT          547 a         1521 c         1531 g          533 t
ORIGIN      
        1 gagagcggct gagccgcctg cgcggctacg ggcgcgcgac ctggtcggcg agccacgcca
       61 ggtacggcgg gtgcccgtcc gccagcggca acgcgatcac ctccggcacc tggtaggggt
      121 gcagctcggt gatcctcgcg gccagcgcgg cgtagcgctc cgccgtggtc ttgatcaccg
      181 ccagcgtctc ggcctcgtca cacacgttgc cctcccagcg atagatcgat cgaaccgccg
      241 gtaccacgtt cacacacgcc gcgagctgct cctcgaccag cgcgcgggcg atccgcgccg
      301 cggtgtcggc atcgggaaac gtcgagaaca ccacgagggc tgcagcgtcg gtcatcctcg
      361 cgctgtacca cgtggtgcgt cgcgtgtagc ttgtcgccgg tgttgatcgt ccggaagtac
      421 ggcggcacgt cggtcggctc gatcgagcgc atgcgcaacg ccgcggcgcg ctgtctggcc
      481 accgcgcgcc agggtcatca ggtcgcggtc gtcgtgtccg cgatgtcggg tgagaccaac
      541 cgcctgctca agctggccgg cgagatccat gaggatccca acgatcgcga ggtcgacgtg
      601 atcgtgtcga ccggcgagca ggtctcggtc ggcctggtcg cgctcacgat ccgcgcggcc
      661 ggcggcaaag gcggcgcgag ggcggccgct gcgccggtcc gtcgcgtcgg gtacagtgca
      721 ggcggatggg ctgcaccaac tgcaagagca agaccggctg cgaccaccgc aagggggaga
      781 tgatcgcgtc ggtcgatcgg acgctcgcgc agctctaccc gacgcggacc tggggcgagc
      841 ccgacgatcg ccagctcgcg gggccggccc ccgacgagct cgccgcgctg gccgacgagc
      901 tcgccggcga gctccacgcg gcgaccttcg tccggcccgg ccgcgacgac gagccgtgcg
      961 actacctcta cgtgctgtgc atgggccgct cgccgtgcgc gatccaggtc cgcgatcacg
     1021 gcgtaccggt gccggcggag tgggcgagcg ccgaggcggg aagcgccgcg atccacgagc
     1081 tgtacctgcg cctggtggtg agccagcgcg cgcggatcgc ggcggtccag caggtcgccg
     1141 tcgaggtcgt gccggccggt cgcggctacc tggtgatcga gcggccgcgc gccggggtgt
     1201 acgacgcgcc gctgttaccc cggatgcaga agctggtcgc gatcctgccg gcgtacgagc
     1261 tcttgcacgt cgacttcggc gagatcgcgc acgcgccgcc gggctatcac gccggggcgt
     1321 ggcgcgagct gttcggcggc gagccggcga tcgcgaacta cctgttctac ccccagccga
     1381 cgacgatgat cgccaccggg ttcatcgacg ccgagggcat cgccgccgag gtcatcgccg
     1441 ccgaggtcag ctcccggatg atacgacgat gagcgacgcc gagatcgccg atgaggtgcg
     1501 cgccgtcctg cgagagctgg tcgacgagat cggcgcgatc tcggcgcgga tcctcgagca
     1561 cgacgagctg cgcaccggcg tgccggcgcg gaccctggcg ctcggcggcg gcgagtacct
     1621 ccgggtcgag ctgccgactc gccacggccg cgaggtcgag gtcgaggccg cgttcgaccg
     1681 caccgtgcgc cagctgcgcg cgatccgccg gcgctgggag atcgcccggc tgcccgagat
     1741 cagcatcgcg cccggagcgc agccgccgag cggccgggtg atcgagcgga tccacggcta
     1801 cctgcgcgcg ctggccagca tcgatcgcgc cagcaacgcg ctggtcaccc gcggcaacca
     1861 gctgatcgcc gcggcgcggc cgcccgacga gctggaggcc gcgcgctggc cgttcctcgc
     1921 ccgccgcgcg ctcgccagcc acgcgccgca cagctcgcac ggcgaggtcg tggaccccga
     1981 cgcctacgcg atgagcttct ggtacagcgc ggtgctgatc gtcctgctcg ccgagccgta
     2041 cgcgctggac ttcgtccgct accggtcgcg ccaggtcgcg cgcgagctcg cccacctgct
     2101 gccgctgctc gagcccgatc ccgacacgcc ggccgcgatc ggccgtccgc cgaccgggcc
     2161 gtagccgggg cccgatcacg gcggcccgat cacggcggcc cgatcgcggc ggagtacagg
     2221 atctgggtcc atgcgccgcc ggcggccggc agcagcagcg cgtcgtgcgg ccacgccggc
     2281 caccggcgat cgatggtcgg gaccaggtcg ccgcccgcgg cgcccgggac ggcgagcttg
     2341 gtcaccgacg catcgggcgg gacctgcggc gccggcacgc cgaggtcggg cgggatcgcg
     2401 ggcagccggg ccacggtcgc cgacggcggg aggtaccagt cccactggaa gtcgccgccg
     2461 acgtgctcga cgatcgtgat cgtgccgccc gccgctccgg tccacgagat ctgcgtcggg
     2521 tcggtgatcg tcatcgactc gaacagcggc agcatgtcct tggcgctgaa ggtcgccgcg
     2581 gtcgcgctcg agagcggcgc gagcagctcg ctcgaggtga tgtactgcgg cggcagcccg
     2641 ccggacgcgc cgatccccag ggtgttaccg ccgggcgcaa cctcctcctg cacggtcacc
     2701 gcgccgccgc tgggcgtgaa gctgtcgata ctcgggctca gcggcgtgag gtcgaggccg
     2761 gcgcgcgcga cgaccccggc ggccacgctg gtggccccgg acgggaggtc ggtcagctgc
     2821 agcgtccgcg tcggcagctg ctggtagctg ccggtgacct gctgggtcgc gccgttgcgc
     2881 agcgtgacca tgccggcgtc gaggtagagg tccggcaagc ccgagccgac ctgggtccgc
     2941 acgaagccga ccgcgtggat ggtggccccc tcgcacgccg gggcgtagtt gaactgcacg
     3001 ctcggcgatc cggagtacgg cggggaggcg cagtgctcgg gcagccgcac tgtgtagtcg
     3061 ctcgcggtgg cgttcgaagg cagcgcgaac gtcatgccgt ggttctggtt gaacgccggc
     3121 cggctgccga gctcgatcgt cccggcgggc ggaggctgca cggtgtagcc gcgccagccg
     3181 atggtgctct ggtagatcac gtgacaggcg atcaccccgg ccgcggcgaa ctgcgcgcgg
     3241 cccgtggcgt cggtcaccat ccggtcggtg ccggtgctgc gctcgaccag cacggtggcc
     3301 ccggcgatcg gcagcccggc gggctcgttg aggtactcgt cgctggtcac caccagcgtc
     3361 accgtgtcgc cggtggcgcc gtcggccgcg gtggcgctgt cggccccggc gtcgctgccg
     3421 ctcggcccgg cgatctcgga aaagccgacc cggccgcagc cggccagggc gatcgccagg
     3481 gcgaaccttg tcgtcgtcat cacatccacg gagcatcacg cgaggcccgg acgcccgcga
     3541 cccccgaccc cgggcaactg ccggctattc gccgagcagc gccttcaaga ctgacgcatc
     3601 gccttccagg gcgcaacgct gcagatacag ccggaggccg cgctcaccac cgagctcctc
     3661 gccgccgccg gcccggccgg gcccgccgtg caggagcgcc ggcagcgccg tccccggccc
     3721 ggggctctgc gacgccatct tcgacgaccc caggtacagc cggccggccc acggcgcggc
     3781 cgacccgatg aaccggccga cccagtcgcg gtcgtcggag tacgccgcgc tgaccaggca
     3841 gccgtcgccg cgcgcgacga acgcggccgc ggcccccgga tcgccggggt aggcgcccag
     3901 cgtgatcacc gggccgaaga cctcctggtc gtggagcgcg gcgcaggtca gcggggactg
     3961 cgtggcgcgc acgaccggcc cgacgaagaa gcccttgccg ggcggggcac cgatcgggcc
     4021 cggggccggg acctcgccgg ttccaccgaa gatcgcctcg gtctcggccg cgagccgcgc
     4081 gatcccggcg cggacgtcgt cgcgctgctg cgcggtcgcc agcgggccca tg
//
LOCUS       DASVOR010001601         4646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_221103,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001601 DASVOR010000000
VERSION     DASVOR010001601.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221103"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF516_30065"
     CDS             complement(<1..481)
                     /locus_tag="VF516_30065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112030.1"
                     /translation="MTSLADAVQAHVAPGMHLHFASTPSRSNAAIREVARAFLGARPR
                     FRISSTGFHSMAHLLPMLGLADRLTACFFGDHYPVSRPNPLYTRLAAEGVVLDHWALW
                     SMVASLRAGAQGDAWVVNRSLRGTTIAEELAARGEYRELSLPEWAELDEPAGFDDGDA
                     "
     gene            complement(848..1546)
                     /locus_tag="VF516_30070"
     CDS             complement(848..1546)
                     /locus_tag="VF516_30070"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8112031.1"
                     /translation="MDSELEIVTIEDHDDEGLLDEFHGGVYWDAFPDQQEPIEVWKRA
                     LWGGAAPYEMTVRIAGTALRDRVRRQILGGIAFERYPRSGCGLVTYMAIAPAARRQGL
                     GKRLQRDAALAMFAAGAPAVFGEVNDPRLAGLGVDEPVEHMWRRLERNQAWGARVVDT
                     RYVQPALAPGLMRDHGLCLIALASEQPLPATLPGRIVRGFVDELYAATEGSAPDGELV
                     ARIPEHVALIELRR"
     gene            complement(1660..2061)
                     /locus_tag="VF516_30075"
     CDS             complement(1660..2061)
                     /locus_tag="VF516_30075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112032.1"
                     /translation="MRAWIALLVLCAGCSAAARLGMSTSTTATRSPAAEVSAGPGGDS
                     ASSMLRPQQLQTLIGLTLDRARARLHELGHRGPIEIQGRNDFGDCAYGHLCGRCEYDQ
                     VCQVRPSGGTDLDGTVVLVVKARAAITVPEP"
     gene            complement(2061..3608)
                     /locus_tag="VF516_30080"
     CDS             complement(2061..3608)
                     /locus_tag="VF516_30080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112033.1"
                     /translation="MLAGVGMLAATGTAWAAPATWCKDVRVDPGALHALESKDPGDLI
                     KALVSAECNPTREIEAHRDELEQARASWSRRLGMTEADWSDAVAYATTPVYAIRAGML
                     ARSLAAASPLDQYAVILNASEPTADMDALYAADLFEPNLSEAGRFAFLERSCVRAAPV
                     DAYGMTGTEVIWAICQPDIDRMDVAKLLAEIHSDTTHDGALRMKLRIDAYELSAALKR
                     HLAEVEQMKARDDASKRLFTLASQAREAWSASAGKNTRLLELVLAMDSAALTQSRKQL
                     EGCEESTAAALAEAVAAIPASSFAGMYDDRGHPDAGFATVAGPVLATSPAVLLAAGAA
                     LRCAPKADLAAYLHDIVDLGAPSRGPRNAALARIKSTRIRYDNANARLVYPQPRPYGG
                     HYLSEIFQARSLGGVVKSVQKHGDTLKVEIKQTFIRTLDCTRSHKTGRISKINADGRV
                     EYEDVCDRSELRTHSDTWNAFELSARYAAWLKPGVMFSATGKDVIAVWPSATAKTPSI
                     VLGGQVH"
     gene            complement(3740..>4646)
                     /locus_tag="VF516_30085"
     CDS             complement(3740..>4646)
                     /locus_tag="VF516_30085"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8112034.1"
                     /translation="TATSRPTPMPTQQPPSAPQAAPQGAQNRPAMNTRPIPRTKEPIP
                     TVGLGTWQAFDVDPSARAPLVEVMRQFLAAGGRVIDSSPMYGRAESIVGDVLGDLGAV
                     GTPFLATKVWTRGKREGIAEMERSRQRMRAERIDLMQIHNLLDWQTHLPVLREMKQAG
                     KIRYLGVTHYAHGELPQIERMMRSEALDFIQIPYNLADRAVEARVLPAAADTGTAVLV
                     MRPFEEGALFRQVKGKPLPGWAAELDCTSWAQIFLKFIIGHPAVTCPIPATADPKHLA
                     DNIQAGFGRLPDAAQRRAMIAALDK"
BASE COUNT          665 a         1679 c         1576 g          726 t
ORIGIN      
        1 gggcatcgcc gtcgtcgaac ccggcgggct cgtcgagctc ggcccactcg ggcaggctca
       61 gctcgcggta ctcgccgcgc gccgcgagct cctcggcgat cgtcgtcccg cgcagcgagc
      121 ggttgaccac ccaggcatcg ccctgggcgc cggcgcgcag gctcgcgacc atcgaccaca
      181 gcgcccagtg gtcgagcacc acgccctcgg cggcgagccg ggtgtacagc gggttgggcc
      241 gcgacaccgg gtagtggtcg ccgaagaagc acgcggtcag ccggtcggcg agcccgagca
      301 tcggcaacag gtgcgccatc gagtgaaagc cggtgctgct gatccggaac cgcggccgcg
      361 cgccgaggaa cgcgcgggcg acctcgcgga tcgccgcgtt cgatcggctc ggggtcgacg
      421 cgaagtgcag gtgcatcccc ggcgcgacat gagcctggac cgcgtcggcg agcgaggtca
      481 ctcgccgtgc gatgccggtt gcgacagcgt cagacatagg ccaccccctt gtcatggatc
      541 ccgtgtcgtc gacctgaccc gacacactat ggtcagcgga atccatttat caagggcaat
      601 atcgcgtgcg cggtgaccgt cgattttctc actgcggatc atccgcagtg ttccagtgtt
      661 ccgcgcctgg ccgaggacgg cctgcacaca acatcgcaac atcgcaggcc ttcgtgcccc
      721 ttgcgatcca tggaacagcg gattgtcgca gaagctcgtg atcctgcgac gccgcggggg
      781 cggcggcccc caggaccgca cgggacgcct acacgcggcc cgccgtgccg gcggtgaccg
      841 cccgcgctca gcgccgcagc tcgatcagcg cgacgtgctc cgggatcctc gccaccagct
      901 cgccgtcggg cgccgagccc tcggtcgccg cgtagagctc gtcgacgaag ccgcgcacga
      961 tccggcccgg caacgtcgcc ggcaacggct gctcgctggc gagcgcgatc aggcacaggc
     1021 cgtgatcgcg catcagcccc ggcgccagcg cgggctgcac gtagcgcgtg tcgaccacgc
     1081 gagcgcccca tgcctggttg cgctccagcc ggcgccacat gtgctcgacc ggctcgtcga
     1141 cgccgagccc ggcgagccgc gggtcgttga cctcgccgaa caccgcggga gcgcccgcgg
     1201 cgaacatcgc cagcgcggcg tcgcgctgga gccgcttgcc caggccctgg cgccgggcgg
     1261 cgggtgcgat cgccatgtac gtcaccaggc cacagccgct ccgcggatag cgctcgaacg
     1321 cgatgcctcc gaggatctgc cgccgcaccc ggtcgcggag cgcggtcccc gcgatccgga
     1381 ccgtcatctc gtagggcgcc gcgccgcccc acagcgcgcg cttccagacc tcgatcggct
     1441 cctgctgatc ggggaacgcg tcccagtaca cgccgccgtg aaactcgtcg agcagaccct
     1501 cgtcgtcgtg gtcctcgatc gtgacgatct ccagctcgct gtccacgccc ctacgctgcc
     1561 acacccgccg gtgcctggcg acgtgtgcgc cactgtcacc cacctcgcct cgcgcgtcct
     1621 gtgcgaggcg cgaaaattcc aggtgtccgg cgtgcccggt cagggttccg ggaccgtgat
     1681 cgcggccctg gccttgacaa ccagcaccac cgtgccgtcc aggtccgtcc caccgctcgg
     1741 ccggacctgg cagacctggt cgtattcaca tcgaccacag aggtggccgt aggcgcagtc
     1801 cccgaagtcg ttcctgccct ggatctcgat cggcccgcga tgaccgagct cgtggagacg
     1861 agcgcgggct cgatcgaggg tcaacccgat cagcgtctgc agctgctgcg ggcgaagcat
     1921 cgaggaagcg gagtcgccgc ccggaccggc gctgacctcc gccgcggggc tccgggtcgc
     1981 cgtggtcgac gtcgacatgc ccaggcgtgc cgcggcgctg caaccggcac acagcacgag
     2041 aagcgcgatc cacgccctca tcagtgcacc tgcccgccga ggacgatgga cggcgtcttt
     2101 gccgtggcac tcggccacac ggcgatgacg tccttgccgg tggcagagaa catcacgccc
     2161 ggcttgagcc acgcggcata cctggccgag agctcgaacg cgttccacgt gtcactgtgg
     2221 gtccgcagct cgctgcgatc acacacgtcc tcgtactcga cgcggccgtc cgcattgatc
     2281 ttcgagattc gccccgtctt gtggctccgg gtgcagtcca gggtccggat gaacgtttgc
     2341 ttgatctcca ccttcagcgt atcgccgtgc ttctgtaccg acttgaccac accgccgagg
     2401 ctgcgggcct ggaatatctc gctgaggtaa tgtccaccgt acggcctggg ttgtgggtac
     2461 accagcctgg cgttcgcgtt gtcatagcgg atccgggtgc tcttgatgcg cgccagcgcc
     2521 gcgttgcggg ggccgcgtga cggcgcgccc agatccacga tgtcgtgcag gtacgccgcg
     2581 aggtcggcct tcggagcgca gcggagagcc gcgccggcag ccagcaggac cgcgggagag
     2641 gtcgcgagca ccggcccggc gacggtcgca aatcccgcat ccgggtgccc gcggtcgtca
     2701 tacatccccg cgaacgagct cgccgggatc gcggccaccg cctcggcgag cgcggccgcg
     2761 gtggactcct cgcagccctc cagctgcttg cgggattgcg tcagtgccgc cgaatccatc
     2821 gcgagcacga gctccaggag cctggtgttc ttgcctgcgc tggcgctcca ggcctcccgc
     2881 gcctgggacg cgagtgtgaa cagtcgcttg ctcgcgtcgt cgcgcgcctt catctgttcg
     2941 acctcggcga ggtggcgctt gagcgccgcc gacagctcgt aggcatcgat ccggagcttc
     3001 atcctgagcg cgccgtcgtg cgtggtgtcg ctgtggatct ccgccaggag cttcgcaacg
     3061 tccatccggt cgatgtccgg ttgacagatc gcccagatca cctcggtgcc ggtcatgcca
     3121 taggcatcga ccggcgcggc ccgcacacag ctccgctcga ggaaggcgaa ccgccccgcc
     3181 tcggacaggt tcggctcgaa caggtccgcg gcgtacagcg catccatgtc cgccgtgggt
     3241 tcactcgcat tcaggatcac cgcgtattga tccagcggtg atgcggcggc caggctcctg
     3301 gccagcatcc cggcccggat cgcatacacc ggcgtcgtcg cataggcgac cgcgtcggac
     3361 caatccgcct cggtcatgcc gagccgcctg gaccacgacg cgcgcgcctg ttcgagctcg
     3421 tcgcgatgcg cctcgatctc gcgggtcgga ttgcactcgg cgctgaccag cgccttgatg
     3481 agatcaccgg gatccttgct ctccagcgcg tggagcgcgc ccggatcgac ccgcacatcc
     3541 ttgcaccagg tcgcgggcgc tgcccatgcc gtcccggtcg ccgccagcat cccgacgccg
     3601 gccaacacaa atcgcttcat agaacgtttc cccttgtgga gttggaatgc ccgttctacg
     3661 agaagcgcgc gcggagctgt ccggtccgga tcgctattga cgggtcatgc acgatttcgt
     3721 gcagacccgt cagcgccggc tacttgtcga gcgcggcgat catcgcgcgg cgctgggccg
     3781 cgtcgggcaa ccggccgaac ccggcctgga tgttgtcggc caggtgcttc ggatccgcgg
     3841 tcgcggggat cgggcaggtc accgcagggt gaccgatgat gaacttcagg aagatctgcg
     3901 cccagctcgt gcagtcgagc tccgccgccc agcccggcag cggcttgccc ttgacctggc
     3961 ggaacagcgc gccctcctcg aacggccgca tcaccagcac cgcggtcccg gtgtcggccg
     4021 ccgccggcag cacgcgggcc tcgaccgcgc ggtcggcgag gttgtacggg atctggatga
     4081 agtcgagcgc ctcgctgcgc atcatgcgct cgatctgcgg cagctcgccg tgcgcgtagt
     4141 gcgtcacgcc gaggtagcgg atcttgccgg cctgcttcat ctcgcgcagc acgggcagat
     4201 gggtctgcca gtcgagcagg ttgtggatct gcatcaggtc gatccgctcc gcgcgcatgc
     4261 gctggcgcga gcgctccatc tcggcgatgc cctcgcgctt gccgcgggtc cacaccttgg
     4321 tcgccaggaa cggcgtgccg accgcgccga ggtcgccgag cacgtcgccg acgatgctct
     4381 cggcgcggcc gtacatcggc gacgagtcga tcacgcggcc gccggcggcg aggaactgcc
     4441 gcatgacctc gaccagcggc gcgcgcgcgg acgggtcgac gtcgaacgcc tgccaggtcc
     4501 cgagccccac cgtcgggatc ggctccttcg tgcgggggat cgggcgcgtg ttcatcgcag
     4561 ggcggttctg cgccccctgc ggagcggcct ggggggcgga gggtggctgc tgggtcggca
     4621 tgggcgtggg cctcgaggtc gcggtg
//
LOCUS       DASVOR010001602         3006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_104971,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001602 DASVOR010000000
VERSION     DASVOR010001602.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104971"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..2267)
                     /locus_tag="VF516_30090"
     CDS             complement(210..2267)
                     /locus_tag="VF516_30090"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8112035.1"
                     /translation="MTTSSRPSAAPAGLAGAPRSGAHPPPRAMFNFGPMPPQKAKNFK
                     QSARRLIAQLAPERAGLIGVLALAVSSVSLAVLIPKLLGLATDVIYRGWLGTPAGEAA
                     SRAAIAAEAAGHASQVDMMRQPGVVPGHGIDFGALAGWLEIALLVAIGASLFTWLQNH
                     VLNHLVQRTVRRMRSDVQAKLARLPLSYFDRQPHGEILSRVTNDIDNVAMGMQQTLSQ
                     ILGSLFNVIGILIAMIVTSPLLAVIALVSVPLSVMATARVARRSQKRFAAQWAHVGAL
                     NGQIEEAFTGHALVKVFGRRREVEDRLRGKNEELFQAGFRAAFLSSVIMPVVQFIANL
                     NFVAIAVVGGLRVASGAMLLGSVQAFIQYSRQFSQHLGQLASMASVLQSGVASAERVF
                     ELLDVEEQTADPPAPAALDRVRGEVRFEHVSFRYKPEQPLIEDLSLVAEPGQTVAIVG
                     PTGAGKTTLVNLIMRFYELDAGRITIDGVDIARLTRRDLRSQIGMVLQDTWLFNGTIR
                     DNIRYGNPAASEAELIAAARATYVDRFVRALPDGYDTVLDDQAGNVSAGEKQLITIAR
                     AFLADPAILILDEATSSVDTRTEVLVQHAMAALRARRTSFVIAHRLSTIRDANVILVM
                     EGGRIVEQGNHAELLAARGAYHALYHAQFAAAVAPDEPAVAGPGPRPGAVARPAVAAP
                     AKS"
     gene            complement(2264..>3006)
                     /locus_tag="VF516_30095"
     CDS             complement(2264..>3006)
                     /locus_tag="VF516_30095"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8112036.1"
                     /translation="PGLLELRGVEYRYPGAELPVLTGVSLTAQPGEITAIVGSTGAGK
                     TTLLELIPRLIDPTSGRVVIEGTDARDIELELLWSKVGVVPQRPYLFSGTVASNLRHG
                     NPAASDDELWAALEVAQARDFVEAMPGRLDAPIAQGGTNVSGGQRQRLSIARALLRKP
                     SIYLFDDSFSALDLATEARLRAALRPLITDATVVMVAQRVASIVDADQIIVLDGGAIV
                     GRGRHAELIETCPTYAEIVASQRAAEAA"
BASE COUNT          434 a         1032 c         1127 g          413 t
ORIGIN      
        1 gcgcatcgac agcgacgtcc gcggcgtggc cgggtacaag gcgggcgact cctacacgct
       61 ctccgagcgc gacatcgtcg actgggtaat cgtccggccc gacggcagcg aggagggcaa
      121 cgtcgtcggc aagttcctcg aagaacgaaa tgccaagcaa caccactgag gcgacgcacc
      181 tgctcgacgg cgggtaggac gaacggtggt cagctcttcg ccggggcggc cacggccggg
      241 cgcgccacgg ctccgggccg cggacctggt cccgcgaccg ccggctcgtc gggtgcgacc
      301 gccgcggcga actgggcgtg gtacagcgcg tggtaggcgc cccgggcggc gagcagctcg
      361 gcgtggttgc cctgctcgac gatccggccg ccctccatca cgaggatgac gttggcgtcg
      421 cggatcgtcg acagccggtg ggcgatcacg aagctggtgc ggcgggcgcg cagcgcggcc
      481 atcgcgtgct ggaccagcac ctcggtgcgg gtgtcgaccg acgaggtggc ctcgtcgagg
      541 atcaggatcg ccgggtcggc gaggaacgcg cgcgcgatcg tgatcagctg cttctcgccg
      601 gcgctgacgt tgccggcctg atcgtcgagc acggtgtcgt agccgtcggg cagcgcgcgg
      661 acgaagcggt cgacgtaggt cgcccgggcg gcggcgatca gctcggcctc gctcgcggcg
      721 gggttgccgt agcggatgtt gtcgcggatc gtgccgttga acagccaggt gtcctgcagc
      781 accatgccga tctgcgagcg cagatcgcgg cgggtgagcc gggcgatgtc gacgccgtcg
      841 atcgtgatcc ggccggcgtc gagctcgtag aaccgcatga tcaggttgac cagggtggtc
      901 ttgccggcgc cggtcgggcc gacgatcgcg acggtctggc cgggctcggc caccagcgac
      961 aggtcttcga tcaggggctg ctcgggcttg tagcggaagc tgacgtgctc gaaccggacc
     1021 tcgccgcgga cgcggtcgag cgcggccggc gccggtgggt cggcggtctg ctcctcgacg
     1081 tcgagcagct cgaacacgcg ctcggccgac gcgacgccgg actgcagcac gctggccatc
     1141 gaggcgagct gaccgaggtg ctggctgaac tggcgcgagt actggatgaa cgcctggacg
     1201 ctgcccagca gcatggcgcc cgaggccacg cgcaggccgc cgaccacggc gatcgcgacg
     1261 aagttcaggt tggcgatgaa ctggaccacc ggcatgatca cgctcgacag gaacgcggcg
     1321 cggaacccgg cctggaacag ctcctcgttc ttgccgcgca gccggtcctc gacctcgcgg
     1381 cgccggccga acaccttgac cagcgcgtgg ccggtgaacg cctcctcgat ctggccgttg
     1441 agcgcgccga cgtgcgccca ctgggcggcg aaccgcttct gcgagcgccg cgcgacccgg
     1501 gcggtcgcca tgaccgacag cggcacgctg accagcgcga tgaccgcgag cagcggcgag
     1561 gtcacgatca tcgcgatcag gatgccgatc acgttgaaca ggctgccgag gatctggctc
     1621 agggtctgct gcatgcccat cgcgacgttg tcgatgtcgt tggtgacccg gctcaggatc
     1681 tcgccgtgcg gctggcgatc gaagtacgac agcggcaacc gggcgagctt ggcctgcacg
     1741 tcgctgcgca tccggcgcac ggtgcgctgc accaggtggt tgagcacgtg gttctgcagc
     1801 caggtgaaca gcgacgcgcc gatcgcgacc agcagcgcga tctccagcca gccggcgagc
     1861 gcgccgaaat cgatgccgtg gccgggcacg acgcccggct ggcgcatcat gtccacctgg
     1921 ctggcgtgac ccgccgcctc ggccgcgatc gccgcgcgcg aggccgcctc gcccgccggt
     1981 gtccccaacc agccgcggta gatcacgtcg gtcgccaggc cgaggagctt ggggatgagc
     2041 accgcgagcg acacgctcga caccgcgagc gccagcacgc cgatcaggcc ggcccgctcg
     2101 ggcgccagct gggcgatcag ccggcgtgcc gactgcttga agttcttggc cttctgcggc
     2161 ggcatcgggc cgaagttgaa catcgcgcgc gggggcgggt gggcgccgga ccggggagct
     2221 ccggcgaggc cggcgggtgc ggcggatgga cggctggacg tggtcatgcg gcctccgcgg
     2281 cgcgttgtga cgcgacgatc tcggcgtagg tcggacaggt ctcgatcagc tcggcatggc
     2341 ggccgcggcc gacgatcgcg ccgccgtcga gcacgatgat ctggtcggca tcgacgatgc
     2401 tcgcgacccg ctgggcgacc atgaccaccg tggcatcggt gatcagcggc cgcagcgccg
     2461 cgcgcagccg cgcctcggtc gcgaggtcga gcgcggagaa cgagtcgtcg aacaggtaga
     2521 tcgacggctt gcgcagcagc gcgcgcgcga tcgacagccg ctggcgctgg ccgcccgaca
     2581 cgttggtgcc gccctgcgcg atcggcgcgt cgagcctgcc cggcatcgcc tcgacgaagt
     2641 cgcgcgcctg ggcgacctcg agcgccgccc acagctcgtc gtcgctggcg gccgggttgc
     2701 cgtggcgcag gttgctcgcg accgtcccgg agaacaggta cggccgctgc ggcacgacgc
     2761 cgaccttgga ccacagcagc tcgagctcga tgtcgcgcgc gtcggtgccc tcgatgacga
     2821 cccggccgct ggtcgggtcg atcagccgcg ggatcagctc cagcagcgtg gtcttgcccg
     2881 cgccggtgct gccgacgatc gcggtgatct cgccgggctg cgcggtcagc gacacgccgg
     2941 tcagcaccgg cagctcggcg ccggggtagc ggtactcgac gccgcgcagc tcgagcaggc
     3001 cgggca
//
LOCUS       DASVOR010001603         2902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395695,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001603 DASVOR010000000
VERSION     DASVOR010001603.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395695"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VF516_30100"
     CDS             <1..523
                     /locus_tag="VF516_30100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112037.1"
                     /translation="VVVVTDQPSVASSLRAEIERLRSPEEAPPVVVTIDSAGALFRAV
                     HAHPRGTTIVIGAAVFSVDDWRSLDANRTRLMRGDVTILILDDDSAGRLENLAPNLAS
                     WVGGRVWRLADGSAAPVLSPGEINQRLTALRAWSGRGDAEVIQLAEAGQLPRDPEYAE
                     WLTLLGRGDLLGS"
     gene            693..1106
                     /locus_tag="VF516_30105"
     CDS             693..1106
                     /locus_tag="VF516_30105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112038.1"
                     /translation="MKKLLRLTPDPKNGPRYWVLTGGPIDFGRTAEPDPGTRFVRTDG
                     GIVHFHLTLHECDARSIEVLAYGFEVYFPSREPIAFVRFDLNFRGHGNDEAGLRSHVH
                     PGNDDLQLPSPVLEPLEALLLVLYGCRPQREVARS"
     gene            1150..1890
                     /locus_tag="VF516_30110"
     CDS             1150..1890
                     /locus_tag="VF516_30110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112039.1"
                     /translation="MWARVDKIDRIRPQPGGGAIVLVEDERSAAAMARTPSLSTLIAI
                     ARILDARRVLELKYRGSGEVRYAAASSPPMFLVDAITRAGAHITDRAADRVKIPAVPA
                     SVSSTIDLAFAELAHHVRTSIGPAVTMPAALRATEERRRKAPIDVDAQPAMFWTAVFE
                     LTALAGELSRPRGGRWIDVPEMPVPFAIRLASGELARPARLAQRIVAGQESVESLAAE
                     APEKAAKADGGAVSGAGNSGGDGGGAPA"
     gene            complement(1906..2637)
                     /locus_tag="VF516_30115"
     CDS             complement(1906..2637)
                     /locus_tag="VF516_30115"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8112040.1"
                     /translation="MIINPNDAAWCRLDGKDCRTRPTPWVRQIILHTTKGDSPQTVLP
                     GKGPGGRAKATAEFWRGDPTPSAAHIVIDNDGTVACLCDLATTETYHATVSNPWSIGI
                     EMYQESGNGIHEAVLDSAVKLVPALCRIFGIQFQIPKAAYKNRPLHRMADGGERCVGV
                     FGHRDNTDRRGHGDPGDEIFERLAAAGAERFDHDAEEDLAAWKSRQNTLVAAGANLEV
                     DGVPGPATCEALKCPQRPHGIWALS"
BASE COUNT          396 a         1010 c         1052 g          444 t
ORIGIN      
        1 ggtggtcgta gtcaccgacc agccgtcggt tgcatcgtcc ttgcgcgccg agatcgaacg
       61 actgcgtagt cccgaggaag caccacccgt ggtcgtgacc atcgattcgg ccggagcgct
      121 gttccgggct gtgcacgctc atccgcgcgg aaccacgatc gtcatcggag cggccgtgtt
      181 ctccgtggat gactggcgga gcctcgatgc gaaccgcacg cgcctcatgc gcggtgacgt
      241 gaccatcctg atcctggacg acgacagcgc aggccgtctc gagaatctcg ccccgaacct
      301 cgcgagctgg gtcggtgggc gggtctggcg gctcgccgat ggctcggctg ctcccgtgct
      361 ctccccgggc gagatcaatc agcggctcac ggcgcttcgc gcctggtccg gccgcgggga
      421 cgccgaggtg atccagctcg ccgaggcagg ccagctgcca cgcgaccccg aatacgcgga
      481 gtggttgacg ctgctggggc ggggagatct tcttggctct tgaagacgca ggatcagagg
      541 ccccgctgac cgcggcgctg cgacggctcc agcaacggct gtcggcagcg acctccgcgt
      601 tgcccgtctc tccgcagggc ctctacgagg aggtacgcgt gctgctgctt cgtgcgatcc
      661 gagaggatgc actgcaaaac gggatgtaca cggtgaagaa gttgctccgg ctcacccctg
      721 acccgaagaa tggtccgagg tactgggtgc tgaccggtgg gccgatcgac ttcggccgca
      781 ccgccgagcc ggaccccggg acgcgcttcg tgcgtacgga tggtggcatc gtccacttcc
      841 acctgaccct gcacgaatgc gatgcgcgga gcatcgaggt tctggcgtac ggcttcgagg
      901 tctacttccc ctcgcgggag ccgatcgcgt tcgtgcggtt cgacctcaac ttccgtggac
      961 acggtaacga cgaagccgga ctgcgctctc acgttcatcc cggcaacgac gacctccagc
     1021 tcccgagccc cgtgctggag ccgctcgagg cactcctcct cgtgctgtac ggttgtcggc
     1081 cgcagcggga ggttgcgcgg tcatgagggt ggcgctggcg ccctgcgcgc catcggatat
     1141 gctggggaga tgtgggcgcg cgtcgacaag atcgaccgga tccggccgca gcccggtggc
     1201 ggggccatcg tgctggtcga ggacgagcgc agcgcggcgg cgatggcgcg gaccccgagc
     1261 ctgtcgacgc tgatcgcgat cgcgcggatc ctcgacgcgc gccgggtgct ggagctgaag
     1321 taccgcggca gcggcgaggt ccgctacgcg gcggcgagct cgccgccgat gttcctggtc
     1381 gatgcgatca cgcgcgccgg ggcgcacatc accgaccgcg cggccgaccg cgtgaagatc
     1441 ccggcggtgc cggcgtcggt gtcgtcgacg atcgacctgg cgttcgccga gctcgcgcac
     1501 cacgtgcgga ccagcatcgg accggcggtg acgatgccgg cggcgctgcg cgcgaccgag
     1561 gagcggcggc gcaaggcgcc gatcgacgtc gacgcccagc cggcgatgtt ctggaccgcg
     1621 gtgttcgagc tgaccgcgct cgccggcgag ctgtcgcggc cgcgcggcgg gcgctggatc
     1681 gacgtgccgg agatgccggt gccgttcgcg atccggctcg cgagcggaga gctcgcccgg
     1741 ccggcccggc tcgcccagcg catcgtcgcc ggtcaggagt ccgtcgagtc gctcgccgcc
     1801 gaggcgccgg agaaggcggc gaaagccgac ggtggcgccg tcagcggcgc gggcaacagc
     1861 ggcggcgacg gcggcggcgc accggcgtag ccgacgccgg gccggtcagc tcagcgccca
     1921 gatgccgtgc gggcgctgcg ggcacttgag cgcctcgcag gtggccggtc ccggcacgcc
     1981 gtcgacctcg aggttggcgc cggcggcgac cagggtgttc tgccggctct tccaggcggc
     2041 gaggtcctcc tcggcgtcgt ggtcgaaccg ctcggcgccg gcggcggcca gccgctcgaa
     2101 gatctcgtcg ccgggatcgc cgtgaccccg gcgatcggtg ttgtcgcggt ggccgaacac
     2161 gccgacgcag cgctcgccgc cgtcggccat ccggtgcagc ggccggttct tgtaggcggc
     2221 cttggggatc tggaactgga tgccgaagat ccggcacagc gccgggacga gcttgaccgc
     2281 gctgtcgagc accgcctcgt ggatgccgtt gccggactcc tggtacatct cgatgccgat
     2341 cgaccacggg ttgctgaccg tcgcgtgata ggtctcggtc gtcgcgagat cacacaggca
     2401 cgcgaccgtg ccgtcgttgt cgatcacgat gtgcgcggcg gacggcgtcg gatcgccgcg
     2461 ccagaactcc gcggtggcct tggccctgcc accgggcccc ttgccgggca gcacggtctg
     2521 cggcgagtcg cccttggtgg tgtgcaggat gatctgccgc acccacggcg tcggtcgcgt
     2581 ccggcaatcc ttgccgtcga gccggcacca cgcggcgtcg ttcggattga tgatcacgag
     2641 accttcaaca gggatctcat tcccgttgac gagaagacct ggcatgtctg tacccctccg
     2701 tcggggcagt gcgccccacg atcgccccca ctggcgatga tggaccagga taccgcatcc
     2761 cgccgcgcga tccacgcgga tcgacgtggc gccccgcgcc gctacgatcc gcggcacctg
     2821 cgggccgccg cggctcgccg gggactcgcc ggctcagcgc gtccggcagc tcgcgagctc
     2881 gagcagccgc cgccgggcgg cc
//
LOCUS       DASVOR010001604         2299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_542619,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001604 DASVOR010000000
VERSION     DASVOR010001604.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2299
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_542619"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..907
                     /locus_tag="VF516_30120"
     CDS             <1..907
                     /locus_tag="VF516_30120"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEX8112041.1"
                     /translation="VSEFLATDDTILYSSCFDANGGLFETLLGEDDAIISDALNHASI
                     IDGIRLCKAERLRYEHDDLADLEAKLKATAGKRLRMIATDGVFSMDGDIARLDAICDL
                     AERHGALVMVDDSHATGFVGRTGRGSPELRGCMDRIDVFTSTLGKALGGASGGFTSGR
                     REIIDLLRNRSRPYLFSNTLAPPVVAGSLAALALISDTTALRDKLEVNTLRFREGMTR
                     AGLQIRPGTHPITPVMLGDARLAVEMARRLLDHGIYVIGFSYPVVPKGAARIRVQLSA
                     AHSTDDVDRAIAAFAAVGKDLGVVR"
     gene            904..1137
                     /locus_tag="VF516_30125"
     CDS             904..1137
                     /locus_tag="VF516_30125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX8112042.1"
                     /translation="MKAEAIAARIRAALPDARVELTDLTGTEDHWQATVISSAFSGKS
                     LIERHRMVMAALAAEMKGPIHALTLHVKAPDEA"
     gene            1181..>2299
                     /locus_tag="VF516_30130"
     CDS             1181..>2299
                     /locus_tag="VF516_30130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112043.1"
                     /translation="MRLFGPEDMGADAAAEELFGPYLVYERLGVGGMATVHRALERGI
                     EGFERIVALKRLLPHLALDATFIKSFVREAKLASVLNHVNIVQMFELGRVGTQYFISM
                     EYIDGRDIRQILRHARKVSGPPPIHVTVGLLLQLCEALDYAHHKNDDVGHPMGLVHRD
                     VSPSNVLVTHAGQVKVIDFGIAKAQSAQLRTQTGRVKGKLAYMAPEALAGKDLDARSD
                     LFAAGVIAHELLTARPLFASKNEYQTLMKVQRGEVMPPSTFNQACPPELDAIVLRALA
                     RDPDDRFPGAGELRDELHAMRKQYNLQTADRDIATWLDWAFQLEAPTEASNGASHGAA
                     GAELDPRARRQRAARQRRQDDDEAVELAWGGHEDHGNGE"
BASE COUNT          353 a          836 c          796 g          314 t
ORIGIN      
        1 ggtcagcgag ttcctcgcga ccgacgacac gatcctgtac tcgtcgtgct tcgacgccaa
       61 cggcgggttg ttcgagaccc tgctgggcga ggacgacgcg atcatctccg acgcgctcaa
      121 ccacgcgtcg atcatcgacg gcatccggct gtgcaaggcc gagcggctgc gctacgagca
      181 cgacgacctc gccgacctcg aggccaagct gaaagcgacc gccggcaagc ggctgcgcat
      241 gatcgccacc gacggcgtgt tctcgatgga cggcgacatc gcccggctcg acgcgatctg
      301 cgacctcgcc gagcgccacg gcgcgctggt catggtcgac gacagccacg cgaccgggtt
      361 cgtcggccgg accggccgcg gctcgcccga gctgcgcggc tgcatggacc ggatcgacgt
      421 gttcacctcg acgctgggca aggcgctggg cggcgcgtcg ggcgggttca cctcgggccg
      481 gcgcgagatc atcgacctgt tgcgcaaccg ctcgcggccc tacctgttct cgaacacgct
      541 cgcgccgccg gtggtcgccg gctcgctggc cgcgctcgcg ctgatcagcg acaccaccgc
      601 gctgcgcgac aagctcgagg tcaacaccct gcggttccgc gagggcatga cccgggccgg
      661 cctgcagatc cgccccggca cccacccgat cacgccggtc atgctgggcg acgcccggct
      721 cgcggtcgag atggctcgcc gcctgctcga ccacgggatc tacgtgatcg ggttctcgta
      781 cccggtcgtg cccaagggcg ccgcgcggat ccgggtccag ctgtcggccg cgcactccac
      841 cgacgacgtc gaccgcgcga tcgcggcgtt cgccgcggtc ggcaaggacc tgggggtcgt
      901 gcggtgaagg ccgaggccat cgccgccagg atccgcgccg cgctccccga cgcccgggtc
      961 gagctgaccg acctgaccgg gaccgaggac cactggcagg cgaccgtgat ctcgtcggca
     1021 ttctccggca agtcgctgat cgagcgccac cggatggtca tggcggcgct ggccgccgag
     1081 atgaagggcc cgatccacgc cctgaccctg cacgtcaagg cccccgacga agcatgatgc
     1141 gtaatgcggc gggttttccg ggtgcgaact gtgcggcgcc atgcgactct ttggcccgga
     1201 ggacatgggc gctgacgccg ccgccgaaga gctgtttgga ccttatctcg tgtacgagcg
     1261 cctcggggtg ggcggcatgg cgacggtgca tcgggcgctg gagcgcggga tcgaggggtt
     1321 cgagcgcatc gtcgcgctga agcgcctgct gccccacctc gcgctggacg cgacattcat
     1381 caagtcgttc gtgcgcgagg ccaagctcgc gtcggtgctc aaccacgtca acatcgtcca
     1441 gatgttcgag ctcggccggg tcggcaccca gtacttcatc tcgatggagt acatcgacgg
     1501 ccgcgacatc cggcagatcc tgcgccacgc gcgcaaggtc tccgggccgc cgccgatcca
     1561 cgtcaccgtg ggcctgctgc tccagctgtg cgaggcgctc gactacgcgc accacaagaa
     1621 cgacgacgtc ggccacccga tgggcctggt ccaccgcgac gtgtcgccgt ccaacgtcct
     1681 ggtgacccac gccgggcagg tcaaggtcat cgacttcggg atcgccaagg cgcagtccgc
     1741 ccagctgcgg acccagaccg gccgggtcaa gggcaagctc gcctacatgg cgcccgaggc
     1801 gctcgcgggc aaggacctcg acgcgcgcag cgacctgttc gccgccggcg tgatcgcgca
     1861 cgagctgctc accgcgcggc cgctgttcgc gagcaagaac gagtaccaga cgctgatgaa
     1921 ggtccagcgc ggcgaggtca tgccgccgtc gacgttcaac caggcgtgcc cgccggagct
     1981 cgacgcgatc gtgctgcgcg ccctggcgcg cgatcccgac gaccggttcc ccggcgccgg
     2041 cgagctgcgc gacgagctgc acgcgatgcg caagcagtac aacctgcaga ccgccgaccg
     2101 cgacatcgcg acctggctcg actgggcgtt ccagctcgag gcgccgaccg aggcctcgaa
     2161 tggagcgtcc catggcgcgg ccggcgccga gctcgacccc cgggcgcgca ggcagcgcgc
     2221 ggcccggcag cgccgccagg acgacgacga ggcggtcgag ctcgcgtggg gcggccacga
     2281 ggaccacggc aacggcgag
//
LOCUS       DASVOR010001605         2440 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_104975,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001605 DASVOR010000000
VERSION     DASVOR010001605.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104975"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VF516_30135"
     CDS             complement(<1..692)
                     /locus_tag="VF516_30135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8112044.1"
                     /translation="MRATSIIYRRELGTYLRSPYGWVIAALLLLIFGLFFQGWAMRGE
                     MLSAFVLERYFYVASGIPLFAGVLLSFRLLSEERQNHSMVLLNTSPVRDSEIVIGKFL
                     AALTFLAVILLLSVYMPILIKVNGKVTTAQVVVGYFGMLLLGAATLAVGLFASALTRQ
                     QLIAAVVAAALVVVMCFIHLLGGAVDPPLKDVLEQIDLWWVHYQYGFMKGILNLKDVI
                     YYVAATYFFLLLS"
     gene            complement(679..1632)
                     /locus_tag="VF516_30140"
     CDS             complement(679..1632)
                     /locus_tag="VF516_30140"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8112045.1"
                     /translation="MIRASNLSKYYGGKRALGPVSFEISDGETVGFLGLNGAGKTTAL
                     RILACDLRPSAGTIEVGGVDAVAEPHEVRKRIGFLPENPPLYSDMVVTDYLRFSGELR
                     GMTKAEVKRRMPEVLEIADLTDVANDQISTLSHGYRQRVGVAQAIVHDPKFLILDEPT
                     RGLDPVQIVEMRNLIHDLKQNHTVLISSHILTEISQTCDRLLVLGRGKILGAGSEQDL
                     ATSESEIRQVTVVVRPPAGDDPRGQIRTVLAAVDGVKTVADPYEQGGGLAFALETTRD
                     CRAEVSRAVVTAKLDLLKLDYARSELENTFIRLVGGADASN"
     gene            1830..>2440
                     /locus_tag="VF516_30145"
     CDS             1830..>2440
                     /locus_tag="VF516_30145"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HEX8112046.1"
                     /translation="MSQVRLAALAMLLVAAGCYTDAPPQSTLPEPQYVSGPPGGGMDP
                     GYATSPYAGPAGGAAMAPGAAEDPGEDDGAAPSEPIPPAAPVGPGAAPGAGPVPDDVA
                     QVGGPPGAPGVPGDPTTTVSDDEIDATLDGYGQWIDTEDYGQVWRPDATVVGVDFTPY
                     ESGGSWAYSDVGWAFVCDYPWGWLPFHYGRWAWFHGYWGWVPG"
BASE COUNT          393 a          854 c          816 g          377 t
ORIGIN      
        1 gacagcagca ggaagaagta ggtcgccgct acgtagtaga tgacgtcctt gaggttcagg
       61 attcccttca tgaatccgta ctggtagtgg acccaccaca ggtcgatctg ctccagcacg
      121 tccttgagcg gcgggtcgac cgcgccgccc agcaggtgga tgaagcacat cacgaccacc
      181 agcgcggccg ccaccaccgc ggcgatcagc tgctgccggg tcagcgcgct ggcgaacagc
      241 ccgaccgcca gcgtcgccgc gccgagcagc agcatgccga agtaaccgac cacgacctgc
      301 gccgtcgtga ccttgccgtt caccttgatc aggatcggca tgtacaccga cagcagcagg
      361 atcacggcga ggaaggtcag cgccgccagg aacttgccga tcacgatctc ggagtcgcgg
      421 accggcgagg tgttgagcag caccatcgag tggttctggc gctcctcgga cagcagccgg
      481 aacgacagca gcacgccggc gaacagcggg atgccgctcg cgacgtagaa gtagcgctcc
      541 aggacgaacg ccgacagcat ctcgccgcgc atcgcccagc cctggaagaa caggccgaag
      601 atcaggagca ggagcgccgc gatcacccag ccatacggcg accgcaggta cgtgccgagc
      661 tcgcggcgat agatgatgct agttgctcgc atcggctcct ccgaccaggc ggatgaaggt
      721 gttctccagc tcgctcctgg cgtagtcgag cttgagcagg tcgagcttgg cggtgaccac
      781 cgcgcggctg acctcggcgc ggcagtcccg ggtggtctcg agcgcgaacg ccagcccgcc
      841 gccctgctcg tacggatcgg cgaccgtctt cacgccgtcg accgccgcga gcacggtccg
      901 gatctggccg cgcgggtcat cgccggccgg cggccggacc accaccgtga cctggcggat
      961 ctcgctctcg gacgtcgcga gatcctgctc gctgccggcg ccgaggatct tgccgcggcc
     1021 cagcacgagc agccgatcac aggtctggct gatctcggtc aggatgtggc tcgagatcag
     1081 gaccgtgtgg ttctgcttca gatcgtggat caggttgcgc atctcgacga tctgcaccgg
     1141 gtcgaggccg cgggtcggct cgtcgaggat gaggaacttc ggatcgtgga cgatcgcctg
     1201 ggccacgccg acgcgctggc ggtaaccgtg tgacagcgtc gagatctggt cgttcgcgac
     1261 gtccgtgaga tcggcgatct ccaggacctc cggcatccgc cgcttgacct cggccttggt
     1321 catcccgcgc agctcgcccg agaaccgcag gtaatcggtc accaccatgt ccgagtagag
     1381 cggtgggttc tccggcagga acccgattcg cttgcggacc tcgtgaggtt cggctaccgc
     1441 gtcgacgccc ccgacctcga tcgtgccggc cgacggccgg agatcgcagg ccaggatgcg
     1501 cagcgccgtg gtcttgccgg cgccgttgag cccgagaaat ccgacggtct caccgtcgga
     1561 gatctcgaac gacaccgggc cgagcgcgcg tttgccaccg tagtacttgg agagattgga
     1621 tgcgcggatc atgcgtcctc gcgtcgcgcc atgggcgcaa aatcgctggc ttgttaatgc
     1681 ttcgcgcggc gttctgtcaa gctgccgcga ggccacaaac cggagccgag gtcgagttct
     1741 tccccgaccc gtgcgttttg tgaacccgga gcgcatgacg agcccgcatg ccgccgcatc
     1801 ggcacaccgg ctgcaggtat cgtacggcca tgagccaggt ccgcctcgcc gcgctcgcga
     1861 tgctgctcgt cgccgcgggt tgttacaccg acgcgccgcc gcagtccacg ctgccggaac
     1921 cgcagtacgt ctcggggcct cccggcggcg gcatggatcc cggttacgcc acatcgccct
     1981 acgccggacc cgccggcggc gccgcgatgg ctcccggcgc cgccgaggat ccgggcgagg
     2041 acgacggcgc ggccccgtcg gagccgatcc ccccagcggc gccggtcggc cccggagcgg
     2101 cccccggtgc cggccccgtt ccggacgacg tcgcgcaggt cggcggacca cctggcgctc
     2161 ccggcgttcc cggcgacccg accaccaccg tctccgacga cgagatcgac gccaccctcg
     2221 acggctacgg ccagtggatc gacaccgagg actacggcca ggtatggcgc cccgacgcca
     2281 cggtggtggg cgtcgatttc acaccctacg aatccggcgg ctcgtgggcc tacagcgatg
     2341 tcggctgggc gttcgtctgc gattacccgt ggggctggtt gccgttccac tacggccgct
     2401 gggcctggtt ccacggctac tggggctggg tccccggcca
//
LOCUS       DASVOR010001606         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_250591,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001606 DASVOR010000000
VERSION     DASVOR010001606.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250591"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2328)
                     /locus_tag="VF516_30150"
     CDS             complement(<1..2328)
                     /locus_tag="VF516_30150"
                     /inference="COORDINATES: protein motif:HMM:NF019311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase-like domain-containing protein"
                     /protein_id="HEX8112047.1"
                     /translation="MVVDPAEQRFRASIVFVLEYLLPQLRERRMRGAVIQALSDTLGL
                     TPPSTARLIETVLHSRRTPGQPLIRDFLALVGSGVTGAYFANAELRGEPALVRTDPEL
                     SFSWVGASPGEGVPARGFSVRWRGFFLPRQKAPHVFYVRTEGAVRLAVKLGGAERVLI
                     DRAAGAAGSARVEELRSDPIALDADQLVELQIEYRNQGGPASFGLQVGTGPRVKQAVA
                     TTDLYPADGLSSFAPVEQSYRRLHKAAMILTGFGTTDAQLEWITGTPPALDLDALPME
                     AGAGPDPVALFTRWRRLAALYALRKKLPRSNVDLFDVFRASSLDEAIERIVLSTGWDR
                     AVVKAFTGTEAPGLGSPTEASEEPLILRLARAVELQRRAGVAPATLFAWAGATPDADM
                     ATTIVQAVKARYDEKRWLEVARTLNDGLRAERRDALVAYLLPRMAEQKVRNRSQLFEY
                     FLIDVDMNPCMMTSRIRQAIGTVQTFFQRCLMNLEPDVPPRLIDDNDWKWLKSYRVWE
                     ANRKVFLYPENWIEPELRDDKSPLFAQLERTILQQEINKDNVEAAFASYLEGLNEISR
                     LDVRGVYFEPRERSRAARFVERRKPTGLQPWEPPPMSWDNGTYHVFARTFNGPHVWYY
                     RRLENARTWTPWEKIDADIEGDHLVPVIFQRRLHLFWSAFREANKPTPPLESPKDSQN
                     KAPSKLGKDWEVSLAYTVYDRGRWSRKRMSTGGVRDVMRLFTPRKDAVDTDGSTALRP
                     GDYTLRATVSGNPARLHLDVYRRKVNGVRTAQRGVS"
BASE COUNT          328 a          840 c          879 g          353 t
ORIGIN      
        1 gctcacaccg cgctgggcgg tgcgcacccc gttgaccttg cggcggtaga cgtccaggtg
       61 cagacgcgcc gggttgccgc tgacggtcgc ccgcagcgtg tagtcgccgg gacggagggc
      121 cgtgctgcca tccgtgtcga cggcgtcctt gcggggagtg aacaacctca tcacgtcgcg
      181 cacgccgccg gtcgacatgc gcttgcgcga ccagcggccg cggtcgtaga cggtgtaggc
      241 gaggctcacc tcccagtcct tgcccagctt ggagggcgcc ttgttctggc tgtccttggg
      301 agactcgagg ggcggcgtcg gcttgttggc ctcgcggaac gcggaccaga acaggtgcag
      361 ccggcgctgg aagatcaccg ggaccaggtg gtccccctcg atgtcggcgt cgatcttctc
      421 ccacggggtc caggtccggg cgttctccag ccggcggtag taccagacgt gggggccgtt
      481 gaaggtgcgc gcgaacacgt ggtacgtgcc attgtcccag gacatgggcg gcggctccca
      541 cggctgcaac ccggtgggct tgcggcgctc gacgaagcgc gccgcccggc tgcgttcgcg
      601 gggttcgaag tagacgccgc gcacgtccag gcgcgagatc tcgttcaggc cctccaggta
      661 gctggcgaac gcggcctcga cgttgtcctt gttgatctcc tgctgcagga tcgtgcgctc
      721 cagctgggcg aacagcggcg acttgtcgtc gcgcagctcg ggctcgatcc agttctccgg
      781 atagaggaag accttccggt tcgcctccca cacccggtag ctcttcagcc acttccagtc
      841 gttgtcgtcg atcaggcgcg gagggacgtc cggctccagg ttcatcaggc agcgctggaa
      901 gaacgtctgc accgtgccga tcgcctggcg gatccgcgac gtcatcatgc aggggttcat
      961 gtcgacgtcg atgaggaagt actcgaacag ctggctgcgg ttccgcacct tctgctcggc
     1021 catgcgcggc agcaggtagg cgaccagcgc gtcccggcgc tcggcgcgca ggccatcgtt
     1081 gagcgtgcgc gccacctcca gccagcgctt ctcgtcgtag cgggccttca ccgcctgcac
     1141 gatggtggtc gccatgtcgg catcgggcgt ggcgccggcc cacgcgaaca gcgtggcggg
     1201 cgcgaccccc gcccggcgct gcagctccac ggcgcgagcc aggcgcagga tcagcggctc
     1261 ctcgctggcc tcggtcggcg agcccaggcc cggggcctcg gtgccggtga aggccttgac
     1321 cacggcgcgg tcccacccgg tgctgaggac gatgcgctcg atggcctcgt ccagcgagct
     1381 cgcgcggaac acgtcgaaca ggtcgacgtt cgagcgcggc agcttcttgc ggagcgcgta
     1441 cagcgcggcc aggcggcgcc accgcgtgaa cagcgccacc ggatcggggc cggcacccgc
     1501 ttccatgggc agcgcgtcca ggtccagggc cggcggcgtg ccggtgatcc actccagctg
     1561 ggcgtcggtg gtgccgaacc cggtcaggat catggccgcc ttgtgcagcc ggcgatagct
     1621 ctgctccacc ggcgcgaacg acgacagccc gtccgccgga tagaggtcgg tggtcgccac
     1681 ggcctgcttg accctgggcc cggtcccgac ctgcagcccg aagctggccg gcccgccctg
     1741 gttccggtac tcgatctgga gctcgacgag ctggtcggcg tccagcgcga tcggatcgga
     1801 gcgcagctcc tcgacgcggg cggagccggc ggccccggcg gcgcggtcga tcagcacccg
     1861 ctcggcgccg ccgagcttca ccgccaggcg gaccgccccc tcggtgcgca cgtagaacac
     1921 gtggggcgcc ttctggcgcg gcaggaagaa gccgcgccag cgaacgctga acccgcgggc
     1981 cgggacgccc tcgccggggc tggcgcccac ccacgagaac gacagctcgg gatccgtgcg
     2041 gaccagcgcc ggctcgccgc gcagctcggc gttcgcgaag taggctcccg tgacgccgct
     2101 gcccaccagg gccaggaagt cgcggatcag cggctggccc ggagtgcgcc gcgagtgcag
     2161 caccgtctcg atcaggcgcg cggtgctggg aggggtcagg cccagcgtgt cgctcagggc
     2221 ctggatgacc gcgccgcgca tccgccgctc gcgcagctgc ggcagcaggt actcgagcac
     2281 gaagacgatg ctggcgcgga accgctgctc ggcgggatcg acgaccacgg gcgcgggggc
     2341 agcggcttcg tcggcggcct cacgcggcgc aggcggcgtg gccggtgggg gctcaggcgg
//
LOCUS       DASVOR010001607         2061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_133236,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001607 DASVOR010000000
VERSION     DASVOR010001607.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133236"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF516_30155"
     CDS             <1..322
                     /locus_tag="VF516_30155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112048.1"
                     /translation="ALWGMASVSLLVSLVFGPAARKFHRRHKLLAREQVYRVRQLAAK
                     DPASAGLGLLHDVSPGLDRHSTAAGWTFALDTVGQVLADAEAEYPSPTFLGMTMDDNG
                     EWSK"
     gene            310..747
                     /locus_tag="VF516_30160"
     CDS             310..747
                     /locus_tag="VF516_30160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112049.1"
                     /translation="MVEVTEQGAPARPTLLDLVHAYGAARENVATMNEGVASGQHPRR
                     HLHVAERRARYLLEQIEQRLAEPVRVLLTNHDAVTILAGRELSLMTADERPAKARLFT
                     PDELLKAATATGERLEAEGLPPGPGMTRADAVRLTNPLKETRS"
     gene            868..1101
                     /locus_tag="VF516_30165"
     CDS             868..1101
                     /locus_tag="VF516_30165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112050.1"
                     /translation="MTASKRGPVRRRRAASKEVRIIERAAKAVRGRVERTANGHLKVF
                     GPLGMTIVGSRLNNARAWSNAARDLRVHAGVVV"
     gene            1298..1528
                     /locus_tag="VF516_30170"
     CDS             1298..1528
                     /locus_tag="VF516_30170"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8112051.1"
                     /translation="MLDERAPMFSGARLRDERQRAGLSRMELAARLGRRWSTIERYER
                     DEAVPSALALGGLAHVLGCTIDDLFEVPDAGH"
     gene            1515..1745
                     /locus_tag="VF516_30175"
     CDS             1515..1745
                     /locus_tag="VF516_30175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112052.1"
                     /translation="MPATDRPATAPSFHDCPGCGAAGVPQHQLACKPCWFRLPERMRD
                     AINHAFRHRLSNPRAHRQAIVRAMTWYRINPT"
     gene            1826..>2061
                     /locus_tag="VF516_30180"
     CDS             1826..>2061
                     /locus_tag="VF516_30180"
                     /inference="COORDINATES: protein motif:HMM:NF020811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEX8112053.1"
                     /translation="MVIPVDTTAGNALHSAALAYAALGWPVFPLRPRLKTPLYGNPHP
                     KDSPERGTCGGWHECGRFGHGVLDATTDRTVIDE"
BASE COUNT          310 a          789 c          685 g          277 t
ORIGIN      
        1 ggcgctgtgg ggcatggcga gcgtgagcct gctcgtgtct ctcgtattcg ggccggccgc
       61 ccgcaagttc caccgccgcc acaagctgct cgcccgcgag caggtgtacc gggtgcgtca
      121 actcgccgcc aaggacccgg ccagcgccgg cctgggcctg ctgcacgacg tcagccccgg
      181 cctcgaccgg cactcgacgg cggccggctg gaccttcgcc ctcgacaccg ttgggcaggt
      241 gctggccgat gccgaggcgg agtacccgtc gccgacgttt ctcggcatga ccatggacga
      301 caacggcgag tggtcgaagt gaccgagcag ggggcgcccg cgcgccccac cctgctggac
      361 ctggtccacg cctacggcgc ggcgcgcgag aacgtcgcca ccatgaacga gggtgtcgcc
      421 agcggccagc acccccgccg ccacctgcac gtcgccgagc ggcgggcgcg gtacctgctc
      481 gaacagatcg agcagcgact ggccgaaccg gtacgagtgc tgctcaccaa ccacgacgcc
      541 gtcaccatcc tggccggccg tgagctgtcg ctgatgaccg ccgacgagcg gcccgccaag
      601 gcgcgattgt tcacccccga cgaactgctc aaggccgcca cggcgaccgg tgagcggctc
      661 gaagccgaag gcctgccccc aggccccggc atgacccgtg ccgacgcggt acggctcacc
      721 aaccccctga aggagactcg atcatgaaac ccaaaggcta tcggggtcgg cacgccgccg
      781 cgccccggct actggtcggc ctcgtccagc tcgtcgcgct cgtcgcggtg gccggcgccg
      841 tcgcgttcgc cctggtcggc tgacgccatg acggccagca agcgcggccc ggtccgcagg
      901 cgacgggcgg cctccaaaga ggtccggatc atcgaacggg cggcgaaagc cgtcaggggg
      961 cgcgtcgagc gcaccgccaa cggccacctc aaggtgttcg gcccgctcgg catgaccatc
     1021 gtcggctccc ggctcaacaa cgcccgagcg tggagcaacg ccgcgcggga cctgcgggtc
     1081 cacgcgggcg tggtcgtcta gactcgacag cgggtttgac gggccacctg cgagcgacgg
     1141 acccccggct tacgaggccg ggggtccgtt gacgtatgtg catcttgtgg agacgtcagt
     1201 ccgggcgtac gctcgccatc ctcccccgtg tgtggtggcc gttggcgagc agtcgccaac
     1261 ccccggaaag gacccccgca tgcccaccat tcccccggtg ctcgacgagc gggcaccgat
     1321 gttctccggg gccaggctgc gtgacgagcg ccagcgcgcc gggctcagcc gtatggaact
     1381 ggccgcccgg ctcggccggc gctggtcgac gatcgagcgg tacgagcggg acgaggccgt
     1441 cccgtcagcg ctcgccctcg gcggactcgc gcacgtgctc ggctgcacca tcgacgacct
     1501 gttcgaggtg cccgatgccg gccactgacc gccccgccac cgccccgagc ttccacgact
     1561 gccccggctg cggtgcggcc ggcgtccccc aacatcagct cgcctgcaag ccgtgctggt
     1621 tccggctgcc cgagcggatg cgtgacgcga tcaaccacgc cttccgtcac cggttgtcca
     1681 atccccgggc gcaccggcag gcgatcgtgc gcgccatgac ctggtaccgg atcaacccca
     1741 cctagcgcgc acggggcgcc cctgcaccag ggcgccccgc accatccccc gagacaagac
     1801 ctcttcagcc cgcaggaggc atcatatggt tattcctgtc gatacgacag ccggaaacgc
     1861 gctgcattcg gccgcgctcg cctatgccgc gctcggctgg ccggtcttcc cgctgcgccc
     1921 ccggctgaag acccccctgt acggcaaccc gcaccccaag gactcgcccg agcgtggcac
     1981 gtgcggcggt tggcacgagt gtggacgctt cggccacggc gtgctcgacg cgaccaccga
     2041 ccggaccgtc atcgacgagt g
//
LOCUS       DASVOR010001608         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_17529,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001608 DASVOR010000000
VERSION     DASVOR010001608.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17529"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1966)
                     /locus_tag="VF516_30185"
     CDS             complement(<1..1966)
                     /locus_tag="VF516_30185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112054.1"
                     /translation="MTQLSTLDRAELTAWLTRVTQTIDRSLRDSARRQAQAALRIGTE
                     GATASVPVLAQLALLDDCLRVAHMAIEADGQIEPDEIARVSALVQVAAPKYFAVLPRY
                     EEFGDGATTPEQIERFFVLHRSDDGAFGYAGPDPWRGLALVRHVERHTHNAAPLRDLE
                     RMLVRIMDDVFAGRAGELERDARRRLRDLFEPPPSLATDPRDTAFCRADGPEVFSSVA
                     HGSQIHERDPFDVESIHGEAREVFHRQIVRATTPEQHQYGHGRTLLVLGDSGSGKTHL
                     LRALRQQVHAQRLGYVGYLQMTSEVGDYARYVLRNLIDSLERPYDAPELAESGLLYLS
                     DGLAEGGSIAGPELDRLRNAELAGAELDDLIGGLVDRILRTDRLSRLEPDLVHALLLL
                     QRRDPALQRRVIRFLRCEPLTGYEQRMLGGLAARDQLEDPMRTIQQLAAIAYELQLAS
                     LVLLVDQVEDTVPDGKTVTRLQQAIDVLRGIADAVPSVVVVMACLDDVYTAVRGQLSR
                     PLIDRLESDPPSVRLASQRQRDDIEAMLVRRLDHLYAAFDVAAQDDDPIYPFSPAQLD
                     AVTLFRARDAIARFREFHEACIAAGAIVGGPGGEPVSERPAPSAETETVAEIDRAWND
                     ALSASGGIPEDDTGVLALVGEAIRGAADELG"
     gene            complement(1963..>2264)
                     /locus_tag="VF516_30190"
     CDS             complement(1963..>2264)
                     /locus_tag="VF516_30190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112055.1"
                     /translation="APAPFAEAVLRAADAVVDGWFGDRKVFVSAVWDQLRRDPRWAGL
                     GLAGFKTDLVAAHRAGELTLARADLVAAMDPALVAASEIAVDGASFHFVVKERAP"
BASE COUNT          299 a          828 c          851 g          286 t
ORIGIN      
        1 cgccgagctc gtcggcggcg ccccggatcg cctcgccgac cagcgcgagc accccggtgt
       61 cgtcctcggg gatcccgccg ctcgccgaca gcgcgtcgtt ccacgcgcga tcgatctcgg
      121 ccaccgtctc ggtctcggcg ctcggcgccg gccgctcgga caccggctcg ccgccgggcc
      181 ccccgacgat cgcccccgcc gcgatgcacg cctcgtggaa ctcgcggaac cgggcgatcg
      241 cgtcgcgcgc gcggaacagc gtcaccgcgt cgagctgggc cggcgagaac gggtagatcg
      301 ggtcgtcgtc ctgggccgcg acgtcgaacg ccgcgtacag gtggtcgagc cggcgcacca
      361 gcatggcctc gatgtcgtcg cgctggcgct ggctggcgag ccggaccgac ggcggatcgc
      421 tctccagccg gtcgatcagc gggcgcgaca gctggccgcg caccgcggtg tagacgtcgt
      481 cgaggcacgc catcacgacg accaccgacg gcacggcgtc ggcgatcccg cgcagcacgt
      541 cgatcgcctg ctgcagccgg gtcacggtct tgccgtcggg cacggtgtcc tcgacctggt
      601 cgaccagcag cacgagcgac gcgagctgga gctcgtaggc gatcgccgcg agctgctgga
      661 tcgtccgcat cgggtcctcg agctgatcgc gcgcggcgag cccgccgagc atgcgctgct
      721 cgtagccggt gagcggctcg cagcgcagga accggatcac gcggcgctgc agcgcggggt
      781 cgcggcgctg gagcagcagc agcgcgtgca cgaggtcggg ctcgagccgg gacagccggt
      841 cggtgcgcag gatgcgatcg accagcccgc cgatcaggtc gtcgagctcg gcgccggcga
      901 gctcggcgtt gcgcagccgg tcgagctcgg ggccggcgat gctgccgccc tcggccaggc
      961 cgtcggacag gtacagcagg cccgactcgg cgagctcggg cgcgtcgtac ggccgctcga
     1021 gcgagtcgat caggttgcgc agcacgtagc gcgcgtagtc gccgacctcg gaggtcatct
     1081 gcaggtagcc gacgtagccg agccgctggg cgtggacctg ctggcgcagc gcgcgcaaga
     1141 ggtgggtctt gccgctgccc gagtcgccga gcaccagcag cgtgcggccg tggccgtact
     1201 ggtgctgctc cggggtggtc gcgcgcacga tctggcggtg gaacacctcg cgggcctcgc
     1261 cgtggatcga ctcgacgtcg aacggatcgc gctcgtggat ctgcgagccg tgcgcgaccg
     1321 acgagaacac ctcgggcccg tcggcgcggc agaacgcggt gtcgcgcggg tcggtcgcca
     1381 acgacggcgg cggctcgaac agatcgcgca gccggcgccg cgcgtcgcgc tcgagctcgc
     1441 cggcccggcc ggcgaacacg tcgtccatga tccggaccag catgcgctcg aggtcgcgca
     1501 gcggcgcggc gttgtgggtg tggcgctcga cgtggcggac cagcgcgagc ccgcgccacg
     1561 ggtccggtcc ggcgtagccg aacgcgccgt cgtcgctgcg gtgcaggacg aagaaccgct
     1621 cgatctgctc gggcgtcgtc gcgccgtcgc cgaactcctc gtagcgcggc agcaccgcga
     1681 agtacttggg cgccgcgacc tggaccagcg cgctgacccg cgcaatttcg tccggttcga
     1741 tctggccgtc ggcctcgatc gccatgtgcg cgacccgcag gcagtcgtcg agcagcgcga
     1801 gctgcgcgag caccggcacc gacgcggtcg cgccctcggt cccgatccgc agcgcggcct
     1861 gtgcctggcg gcgcgcggag tcgcgcaggc tgcggtcgat cgtctgggtc acgcgggtca
     1921 gccaggcggt gagctcggcg cgatcgaggg tcgacagctg ggtcacgggg cccgctcctt
     1981 gacgacgaag tggaagctcg cgccgtcgac cgcgatctcc gacgccgcga ccagcgccgg
     2041 atccatggcg gcgaccagat cggcgcgcgc cagggtgagc tcgccggcgc ggtgggcggc
     2101 gaccaggtcg gtcttgaacc cggccagccc gagccccgcc caccgcggat cgcggcgcag
     2161 ctggtcccac accgccgaga cgaacacctt gcgatcgccg aaccagccgt cgaccaccgc
     2221 gtcggcggcg cgcagcacgg cctcggcgaa cggcgccggc gcat
//
LOCUS       DASVOR010001609         2276 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_133267,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001609 DASVOR010000000
VERSION     DASVOR010001609.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133267"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VF516_30195"
     CDS             <1..689
                     /locus_tag="VF516_30195"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HEX8112056.1"
                     /translation="PLAPTPTAPDDLAAAVALARAAADAGEQDDVWFARAQWLVRKAL
                     LARSRELDLREDDIFWIPLDDVVTLRTLDPDDVHRRASAARAAHARAAEWDMPLTVQG
                     SDECGESPRAATLRGVGVGPRTIGRVVRFASLAAARPVSREDVVVTRAVTPALAMLVD
                     GCAALVSETGGLLDHGAALARELGATCVVGCAGAWTALGDGAQVSVDGDAGLVTLIPD
                     RISDRPPDGA"
     gene            complement(719..1084)
                     /locus_tag="VF516_30200"
     CDS             complement(719..1084)
                     /locus_tag="VF516_30200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEX8112057.1"
                     /translation="MTKSELIEKISETLKLPAGKAEAIVNTIFDSMVKALERGEGIEI
                     RGFGSFTVRKYKAYEGRNPRTGETVHVAEKRLPFFKVGKDLRERVNAGLGTPLPADKP
                     DEPDDTDPNEPEYDEEEEE"
     gene            complement(1154..1654)
                     /locus_tag="VF516_30205"
     CDS             complement(1154..1654)
                     /locus_tag="VF516_30205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112058.1"
                     /translation="MGKTARVEIANAIGSGIADVVVTAPVRAACNAASARAFHAMTIR
                     PNGREMQVECACHEGIARAVARVETVEVLPLSAWYLDPVRPSAGGTEPRIARPPAAGD
                     RPRRARSRCPGRCEVGSNAAKSGGEISSTSTLGTAARLASFPCYIDSLDETANEACQP
                     SPRACD"
     gene            1964..>2276
                     /locus_tag="VF516_30210"
     CDS             1964..>2276
                     /locus_tag="VF516_30210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112059.1"
                     /translation="MARAYRHRRSPTVGHRALPLVLAVSFTVLGAFALGIVALRPAPA
                     YAEDRVAALGRMLGSSSEKVRLSAVLALAKLGEPRVAGPLIRALHGRRLAPRSRTRSD
                     RT"
BASE COUNT          319 a          836 c          746 g          375 t
ORIGIN      
        1 ggccgctcgc gccgacgcct accgctccgg acgatctggc ggccgcggtc gcgctcgccc
       61 gcgcggctgc cgatgccggc gagcaagacg acgtctggtt cgcccgcgcc cagtggctgg
      121 tgcgcaaggc gctgctggcg cggtcgcgcg agctcgactt gcgcgaggac gacatcttct
      181 ggatcccgct cgacgacgtg gtaaccctgc gaaccctcga tcccgacgac gtgcaccgcc
      241 gcgcctcggc cgcgcgcgcg gcgcatgctc gcgccgcgga gtgggacatg ccgctcacgg
      301 tgcaaggcag cgatgagtgc ggcgaatcac cccgcgcggc cacgcttcgc ggagttggcg
      361 tcggcccgcg cacgatcggt cgcgtcgttc gcttcgcctc gctcgccgcg gcgcgcccgg
      421 tgtcgcgcga ggacgtggtg gtgacccgcg ccgtgacgcc ggcgctcgcg atgctggtcg
      481 acggctgcgc ggcactcgtc agcgaaaccg gagggttact cgatcacggc gccgcgctcg
      541 cgcgcgagct cggcgcgacg tgcgtcgtcg gctgcgccgg tgcgtggacc gcgctcggcg
      601 acggcgcgca ggtctccgtc gacggcgatg ccggcctcgt caccctgatc cccgaccgga
      661 tctccgatcg accgccggac ggcgcgtaac ctggcgccgt ctcttcgcta ctcttcctct
      721 actcttcctc ttcctcgtcg tactccggct cgttcggatc ggtgtcgtcg ggctcgtcgg
      781 gtttgtccgc cggtagcggc gtcccgaggc ccgcgttcac tcgctcccgc aagtctttcc
      841 ctaccttgaa gaatggaagc cgcttctcgg cgacgtgaac ggtctcgccg gtgcgcgggt
      901 tacgcccttc gtaggccttg tacttgcgaa ctgtgaagct gccgaagccg cggatctcga
      961 tgccttctcc acgctccagt gccttcacca tcgagtcgaa gatcgtgttg acgattgcct
     1021 cggcttttcc ggctggcagc ttcaacgttt cagagatttt ctcgatgagc tcggacttgg
     1081 tcacccgtaa ctccgcgtaa ctaggactag ctaaacagtc ctcgagggca atagcaagct
     1141 taaccacgcg gaatcagtca catgcccgcg gagacggctg gcacgcctcg ttcgccgtct
     1201 catctaagct atctatataa caagggaaac tggcaagtct ggccgcggtg ccgagcgtgg
     1261 acgtcgatga gatctcgcca cccgatttcg ccgcgttcga gcccacttcg caccgtccgg
     1321 ggcatcgcga acgggcacgg cgcggccggt ctccggcagc gggtggccgc gcgatccgcg
     1381 gctcggtgcc gcccgcggag ggccgaacag ggtccaggta ccaggccgac agcggcagca
     1441 cctcgaccgt ctcgacccgc gccacggccc gcgcgatgcc ctcgtggcag gcgcactcca
     1501 cctgcatctc gcggccgttc ggacggatcg tcatcgcgtg gaacgcccgc gcggaagcgg
     1561 cgttgcatgc cgcccggacc ggcgcggtca ccacgacatc ggcgatgccc gatccgatcg
     1621 cgttcgcgat ctcaacgcga gccgtcttcc ccatggatcg cgcgaagcag gaccccggcg
     1681 atctcccgcg gcggccgtcc gacctcccgc acagggcgcg cgtcatcgac ccggtacggc
     1741 aacttgcgcg acagctcgtc gagcgcagac gcacggcccc ctccgatccc gaccacgggt
     1801 gcaatgccag ccaccgacgc cgatgcggaa ctgcgtctcc gtgatcgata ctgacttcac
     1861 catacacgcc actgtgcgca ggcgccgcat cggccatgca caaaccgtgc agcgcgaagc
     1921 ggttgactcg ctcgacgcgg tcggccctaa tttgatccaa ggcatggcca gggcgtaccg
     1981 acaccgccga tcgcccacgg tcggccaccg cgccctgccg ctggtgctcg cggtgtcgtt
     2041 caccgtcctc ggcgcgttcg cgctcgggat cgtggcgctc cggcctgcgc cggcgtacgc
     2101 cgaggatcgc gtcgccgcgc tcggccgcat gctcggcagc tccagcgaga aggtccggct
     2161 gtccgcggtg ctcgcgctcg ccaagctcgg cgagccgcgg gtcgcggggc cgctgatccg
     2221 cgcgctccat ggaagacggc tcgcgccgag atcgcgaacg cgatcggatc ggacat
//
LOCUS       DASVOR010001610         7972 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_278621,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001610 DASVOR010000000
VERSION     DASVOR010001610.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7972
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278621"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2493
                     /locus_tag="VF516_30215"
     CDS             <1..2493
                     /locus_tag="VF516_30215"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8112060.1"
                     /translation="RTVTVASTPAVLDVQVEGPGTGEVITIIGSRTPRSRLETPVPVD
                     VLGSDVINESGHTETNQTLNVIAPSFNASHLSVTDGTDHIDPADLRGLGPEHVLVLIN
                     GKRLHQSSLINVYNGGTVGVDLNAIPTSAIARIEVLRDGAASQYGSDAIAGVINIVLK
                     DTVDLVDLYTLAGVTAARDGAQLRLGGNTGMKLGDHGFVNVTGEFFSRGRTNRAGPYA
                     GTIFKDASGNDITDAELQRRGLTRDDFKMDIGTAGARVGTGFLNAGYKLDSTFELYAQ
                     GGYTYRKGYASGFYRLPNADFDDRNDLRIYPDGFLPQIDPVLNAWTATAGVRGKSGPW
                     QGDASLTYGGDSFHFLIDHSLNASLGLASPTRFDAGKLGLTQTSLNFDGVRRIDQGAL
                     HALSLVAGAELRRESYYIRAGQPESYELGPEVDSSGQPKSPGSQVFPGFRPSDASNDS
                     RRSEAAYAGVESQPTALTNLDLGARFEHYSDFGNTLTGKVAGRVAVVRTNDSELALRG
                     SASTGFRAPGLQQIYYSTITSQFVNDPVTHMVVPNNVLISPNRSELTEKAFGVPNLKQ
                     ETSVNFSGGITARLNNNLSVSTDYYHVTLRDRVVLSSLFSAEDPVDQGKFGEAVKTIL
                     MPFDGVSAAQFFVNAVDTTTDGVDVVVDHSAHLPRGMLKTTAAANFTHTVVDDVHVPQ
                     SMQAKFSAIQGGSDHVKELFLGRYGRNLLEDLLPRRKGTLGLRWDDESWTAGLRANYF
                     GPTRYHSDSGPELDESYGAKVIFDADVGYRVHGVSIIVGASNLFNTFPDPMKHEDNRY
                     YNSFLYSPAAASAGTPFGIDGAFYYMRMEYQH"
     gene            complement(2584..3117)
                     /locus_tag="VF516_30220"
     CDS             complement(2584..3117)
                     /locus_tag="VF516_30220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112061.1"
                     /translation="MDRIERFLEEPVAVLELEEGAEVIGITEGTPRSKTDRVMLFDVG
                     RDLCRWLLDNTSAGLVLDPSTPIETRLTREQLAAIVELDREETYSLLASTLVVPRNEQ
                     TGAPIGTREGFIVVFRDPAAYGCWKPPVPCSGHEIPARDVFENLITTDLNGVVIDPDS
                     SNERWIRREGLASLLRA"
     gene            complement(3208..3750)
                     /locus_tag="VF516_30225"
     CDS             complement(3208..3750)
                     /locus_tag="VF516_30225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112062.1"
                     /translation="MKRSRRKKPLPGKQVPRGNSIPFHRELAAMQIHCDQMHAALDWK
                     DPGRVRSTACHVALAWLELEAAFIRFIERTPAGSGLGSKAWRERLTLTGSNHLVRALF
                     QRALDETPRWTASRRELHALRVVLRWLHERLQLWSVVELPVLHRSAKCEYTQETVEPI
                     ASPDPDRPEPIAPKSRRKRP"
     gene            complement(4054..4551)
                     /locus_tag="VF516_30230"
     CDS             complement(4054..4551)
                     /locus_tag="VF516_30230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112063.1"
                     /translation="MALAEQAFQTSLILPLGQEDAPLDPDFVSRLFDARCGVRLIALA
                     ASPDIARRALTDANVELMQHIPVRALAYRSGEIHALLDRQFTERAFHLRADDLTPVNQ
                     DALKTYHWPGNFAELREIADAIIAHATQGGMRRAAASLGVSRQKLTRRLARVGLTLPL
                     FCQDE"
     gene            5555..>7972
                     /locus_tag="VF516_30235"
     CDS             5555..>7972
                     /locus_tag="VF516_30235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112064.1"
                     /translation="MPDRDLIGRTLGPYTLRELIAEGGYGAVYRGEEPVLEREVVVKV
                     LHERRSDSESRERFLREARLASQLDHPYATHIYAYGAEDEGRLLWLAMEWVRGVPLDV
                     WLDRQGPMSPESFGRFFEGLCDVLHVAHKQGIIHRDLKPSNIMVIECAGRLLPKLLDF
                     GIAKWHRHRELAPGPGSDEDRDPDHNDVKTVRMPVRPRRAWRTVTCHDSESRRQLTPP
                     GGYVGSPPYMALELWVGADAAGPEADIYALAIIAYETLTGRRLFVANRTSEYFEQHKC
                     ASAPPPLGEGFPPALDLVIRCALDKDPRARPATALELASHVRTALRKIKREQLRTSAQ
                     QWSDQARAPGLLWGADVLADTLRSVPQQTLSPLERSFVAASERRIRRIRRARRALVGL
                     AAMVVIAGVLYRAMMQTRVTEATITQAELEQGRSALLHNEPDARRHLAEAYRRDHSPS
                     TAFMLARALQPRLAEQARFASTFERMWSAVFSPNGKQLVTTDDRNAQIWDAQSYRLLF
                     TLNHGDMVYHAVYSADGTRVVTGCGDGAVRIWDAVRGVLLREFRRDKGKLHYYVVALS
                     PDGKLVAGLDLGVAHVWEVDTGTPIAELRDAEPSSFPSLAFSTDGRWLAMSTGDDVQV
                     FEAKTWTVVHNISGLGIHSLSWDPTGPHLVTGSAEGDASVWVMPSGARAHHLRELGES
                     IDAVAFSPDSRLIVAGSRDGSVQVWDATSGKLRSQNNYLRSEILSVEFNRNSSLIAAS
                     GSNGSVAVMDAASGMPVTNLDGPTTKVRVAHFDPSSQRVVGASWDGTARVWDATAQYR
                     HWSAPPNN"
BASE COUNT         1402 a         2696 c         2571 g         1303 t
ORIGIN      
        1 aggacggtga ccgtggcatc gacccccgcc gtgctcgacg tccaggtcga gggcccgggc
       61 acgggcgagg tgatcacgat catcggctcg cgcacgccgc gcagccggct ggagaccccg
      121 gtgccggtcg acgtgctcgg cagcgacgtg atcaacgagt ccggccacac cgagaccaac
      181 cagacgttga acgtgatcgc accgtcgttc aacgccagcc acctcagcgt caccgacggc
      241 actgaccaca tcgaccccgc ggatctgcgc ggcctcggcc ccgagcacgt cctggtcctg
      301 atcaacggca agcgcctgca ccagagctcg ctgatcaacg tctacaacgg cggtacggtc
      361 ggcgtcgatc tcaacgcgat cccgaccagc gcgatcgccc ggatcgaggt cctgcgcgac
      421 ggcgccgcgt cgcagtacgg ctcggacgcg atcgccggcg tgatcaacat cgtgctcaag
      481 gacaccgtcg acctcgtcga cctctacacc ctggccggcg tcaccgcggc cagggacggc
      541 gcgcagctca ggctcggcgg caacaccggc atgaagctgg gcgatcacgg gttcgtcaac
      601 gtcaccggcg agttcttctc gcgcggacgg accaaccgcg ccgggccgta cgcaggcacc
      661 atcttcaagg acgccagcgg gaacgacatc accgacgccg agctccagcg ccgcggcctg
      721 acccgcgacg acttcaagat ggacatcggc acggcgggcg cccgggtcgg gaccggcttc
      781 ctcaacgccg ggtacaagct cgacagcacg ttcgagctct atgcccaggg cggctacacc
      841 taccgcaagg gctacgcctc cgggttctac cggctaccca acgccgactt cgacgaccgc
      901 aacgatctac ggatctaccc cgacggcttc ctgccccaga tcgatccggt gctcaacgcc
      961 tggacggcca cggccggggt gcgcggcaag tcgggcccgt ggcaaggcga cgccagcctg
     1021 acctacggcg gcgacagctt ccacttcctc atcgatcaca gcctgaacgc gtcgctcggc
     1081 ctggcgagcc cgacccgctt cgatgccggc aagctcgggc tgacccagac cagcctcaac
     1141 ttcgacggcg tgcgccgcat cgatcagggc gcgctccacg cgctgtcgct ggtcgccggc
     1201 gccgagctcc gccgcgagag ctactacatc cgcgccggcc agccggagtc gtacgagctc
     1261 gggcccgagg tcgactcgtc cggccagccc aagtcgccgg gctcccaggt gttccccggg
     1321 ttccggccat ccgacgcgtc gaacgacagc cggcgcagcg aggcggccta cgccggcgtc
     1381 gagagccagc cgaccgcgct caccaacctc gacctcggag cccgcttcga gcactacagc
     1441 gacttcggca acacgctgac cggcaaggtc gccgggcgag tcgcggtggt ccggaccaat
     1501 gacagcgagc tggcgctgcg cggctcggcg tcgaccggct tccgcgcccc gggactgcag
     1561 cagatctact acagcacgat caccagccag ttcgtcaacg atccggtgac ccacatggtg
     1621 gtgcccaaca acgtcctgat cagccccaac cgcagcgagc tcaccgagaa ggcgttcggc
     1681 gtccccaacc tcaagcagga gacctcggtc aacttcagcg gcggaatcac cgcccggctg
     1741 aacaacaacc tgtcggtcag cacggactac taccacgtga ccctccggga tcgcgtcgtg
     1801 ctctcgtcgc tgttcagcgc ggaggacccc gtcgaccagg gcaagttcgg cgaagccgtc
     1861 aagaccatcc tgatgccgtt cgacggcgtc agcgccgcgc agttcttcgt caacgcggtc
     1921 gacaccacga ccgacggcgt ggacgtcgtg gtcgatcaca gcgctcacct gcccaggggc
     1981 atgctcaaga cgaccgccgc cgcgaacttc acccacaccg tggtggacga cgtgcacgtg
     2041 ccacagagca tgcaggcgaa gttcagcgcg atccagggcg gctcggacca cgtcaaggag
     2101 ctgttcctcg gccgctacgg caggaacctg ctcgaggacc tcctgccccg gcggaaggga
     2161 acgctcgggc tgcgctggga cgacgagagc tggactgccg gcctgcgcgc gaactacttt
     2221 ggccccacca ggtaccacag cgacagcggc cccgagctcg acgaatcgta cggcgccaag
     2281 gtgatcttcg acgccgacgt cggctaccgg gtccatggcg tgtcgatcat cgtgggcgcg
     2341 agcaacctgt tcaacacgtt ccccgacccg atgaagcacg aggacaaccg ctactacaac
     2401 agcttcctgt acagcccggc ggcggcctcc gccggcacgc cgttcggcat cgacggcgcg
     2461 ttctactaca tgcgcatgga gtaccagcac tgacgcgatc accaggtcaa cgtattgcgc
     2521 cgccgcagca agcgccccac gcttgaggaa tcccgcgggg gctgcgatgt cagctccgcg
     2581 cggttaggcg cgaagtaacg acgccagccc ttcacgccga atccaccgct cgttcgatga
     2641 gtcggggtcg atgacgacgc cgttcagatc ggtggtgatg agattttcga acacgtcgcg
     2701 cgcggggatc tcatgcccag agcatggcac gggcggcttc caacaaccgt atgcagcggg
     2761 gtcgcggaac acgacgatga agccctcccg tgtcccgatc ggcgcaccgg tctgctcatt
     2821 gcgcggcacg acgagggtcg acgccaagag cgagtacgtt tcctcgcgat cgagctcgac
     2881 gatcgccgcc agctgttctc gagtcagccg ggtttcgatc ggcgtgctcg gatcgaggac
     2941 caggccagca ctcgtgttgt ccagcagcca gcgacaaaga tcgcgcccga cgtcgaagag
     3001 catcacgcga tcggtcttcg aacgcggcgt gccttcagta atcccgatca cttcggcgcc
     3061 ttcctcaagt tcgagcacgg ccacgggttc ctcgaggaag cgttcgatgc ggtccatggc
     3121 ggcaagtgta catcgactcg cccgacaatc gctggttccc gaacacggcg tcgcaaacgg
     3181 tcccgtcgtg gtgtggcgtc aacgagatca tggccgcttg cggcgtgact tcggtgcgat
     3241 gggctcgggg cggtcgggat cgggcgaagc gatcggctcc accgtctcct gtgtgtattc
     3301 gcatttcgcg ctgcgatgca agacgggcaa ttccaccaca gaccacaact gcaagcgttc
     3361 atgaagccag cgcaagacga ctcgcaaggc gtgcaattca cgtcgagatg cggtccaacg
     3421 aggcgtttcg tccaacgcac gctgaaacag cgctcttacc aggtgattgc ttccggtcaa
     3481 tgtcaatcgc tcgcgccagg ctttggaccc cagtcctgac cctgcagggg tgcgctcgat
     3541 gaaccggatg aaggcggctt ccagctccag ccaggccagc gcaacgtgac atgcggtgct
     3601 ccgcactctg ccaggatcct tccagtcgag tgctgcatgc atctgatcac aatgtatctg
     3661 catggcagcg agctcacgat ggaacgggat cgagttaccc ctggggactt gcttgcctgg
     3721 cagaggcttc ttacgccgtg aacgcttcat gtattcatcc caaccgcgcg gaatggcgag
     3781 gcatccggcg cccgagccaa cgcgagctgc cgcctgccca ttccagcgca gcatcccttt
     3841 gccgctttgt cgaatagctc gccacggcac tacagaacac gaaatcatgg ccaatgaaaa
     3901 gggatggtcc gcgaatgcat attcgcgtcg atttcgtgga ccgggcagat ggacccgtcg
     3961 ctctgtaaat gtcgaggatt gcgccggcga gcgttcctcc gcgtggctcg ccctgtgagc
     4021 ttgcgctccg gtatcgtggg ccaggttcgc cggctactcg tcctggcaga acagcgggag
     4081 tgtcagcccg acgcgtgcaa ggcgccgcgt caacttctga cgcgagaccc ccagcgacgc
     4141 cgcggcgcgg cgcatgccgc cctgcgtggc gtgcgcgatg atcgcgtcag cgatctcgcg
     4201 cagctcggcg aagttgcccg gccagtggta cgtcttgagc gcgtcctggt tcaccggcgt
     4261 caggtcgtcg gcgcgcaggt gaaacgcgcg ctccgtgaac tggcgatcca gcagcgcgtg
     4321 gatctcgccg ctccggtatg cgagcgcgcg caccgggatg tgttgcatca gctcgacatt
     4381 ggcatcggtc aacgcacgcc gcgcgatgtc cggactcgcc gcaagcgcga tgagccgaac
     4441 gccgcagcgt gcatcgaaca gcctcgacac gaagtctgga tcgagtggtg catcctcctg
     4501 ccccagggga aggatcagcg aggtctggaa ggcttgctcc gccagcgcca cctgggcctc
     4561 tcccgccgga ggcaccgtgg cgacctcgac cgccttccgg ttccgccgtg aagacatcgc
     4621 gtggatcgca tgtgcaaggc gatcgagatc gcatccagct ttacccgtga gcagcatcgg
     4681 ccctgaaccc gtcgccgact cgaccatcac ccagtccgga gtccggacag cgcgcgcccc
     4741 caagatctcg aacaccgtgg gacgatgctg ccgcatctca tcgttgaggc agacgaatgt
     4801 catcggacgt gccgtgaaca tgtctccggg gctcaggtac gctgagcctt cgagcctccg
     4861 ctcccgaacg aagattccgt acgagctgtc gaggtcgtgc agccgcagct tgcgcgcacg
     4921 acgctccagg aggcagtgcc gagccgacag ccctcggccc gggatcgaga tcgtgcactg
     4981 gctcgatccg ccgaggctga actcggcaag ttctccacgc agctcccagt ccccggtgaa
     5041 gttgtactca cagatcgcgg tgattggatc gggtcccgcg tcccatccca gaaaccgatc
     5101 gttggggggc ggcggtgtcc gtatcggcgt cacgggggat gctggcgagt tcctcggtgt
     5161 caccgagccg agccggaggg agctggatgc ctcgggtgga gccggcacac gtgggcgggg
     5221 cctgtcggta ggatccatgg gatcagagag cacttcaagg taggacgctg caaggggtgg
     5281 cccgccgaat actcgatttc cgagttttgc gcgtcatctt ggctgtgctc gcaactctgg
     5341 atcgcggtgt tgtcgccggg gctagtagtc tcgcccacgg agaatgcctc gcggctggcc
     5401 cggaatcttg cctggagcca cggttctgcg ggccacctct tgaatcgccg cgatggacgc
     5461 atctcacgct gctgcagcaa aatgcgtcat gcaacaaacc tctcagtagg tgaagggtgg
     5521 ctagcctatc cccccacgga gggagtcaac gctgatgcca gatcgcgatc tgattgggcg
     5581 cacgctgggc ccgtacaccc tgcgcgagct gatcgcggag ggcggctacg gggcggtgta
     5641 ccgcggcgag gagccggtgc tggagcgcga agtggtggtc aaggtcctgc acgagcggcg
     5701 aagcgacagc gagtcgcgcg agcgcttcct tcgcgaggcg cggctcgcgt cacagctgga
     5761 ccacccctac gcgacgcaca tctacgccta cggcgccgag gacgaaggca ggctgctgtg
     5821 gctcgcgatg gagtgggtac gcggcgtccc gctcgatgtc tggctagaca ggcaggggcc
     5881 gatgtcgccc gagtcgttcg gtcggttctt cgagggcctc tgcgatgtcc tccacgtcgc
     5941 gcacaagcag gggatcatcc accgcgacct gaagccgtcc aatatcatgg tgatcgagtg
     6001 cgcggggcgt ctgctcccca agctcctcga tttcgggatc gccaagtggc atcgacatcg
     6061 cgagctggcc cctggccctg gctccgacga ggatcgcgat cccgaccaca acgatgtcaa
     6121 gaccgtgcgc atgccggtcc ggccgcgccg cgcgtggcgc accgtcacct gccatgactc
     6181 ggagagcaga cgccagctca cgccgccggg tgggtacgtc ggctcgcctc cgtacatggc
     6241 tctggagctg tgggtcggcg ccgacgccgc cgggccggag gccgacatct acgcgctcgc
     6301 catcattgct tacgagacgc tgaccgggcg ccggctgttc gtcgcgaacc ggacgtccga
     6361 gtacttcgaa cagcacaagt gtgcgtctgc accaccgccg ctcggcgagg gcttccctcc
     6421 ggccctcgat ctggtcatcc ggtgcgcgct cgacaaggat ccccgggctc gcccggccac
     6481 cgcgctggag ctggcctcgc atgtgcggac ggcgctgcgc aagatcaagc gggagcagct
     6541 ccggacctcg gcgcagcagt ggtccgatca ggcgcgggct ccgggcctgc tgtggggggc
     6601 cgacgtgctg gcggacaccc tgcgcagcgt gccccagcag acactcagcc cgctggagcg
     6661 ctcgttcgtc gccgcgagcg agcggcgcat ccggcgcatc cgccgggctc ggcgggcgct
     6721 cgtcgggctc gccgcgatgg tcgtgatcgc cggagtcctg taccgcgcca tgatgcaaac
     6781 ccgcgtgacg gaggcgacca tcacgcaggc cgagcttgag cagggtcggt cggcacttct
     6841 ccataacgaa cccgatgcac ggcgccatct cgccgaggcg taccgccgtg atcactcgcc
     6901 gtcgaccgcg ttcatgctgg cgcgcgcgct gcagccgcga ctcgccgaac aggcgcgctt
     6961 cgcgagtacg ttcgagcgca tgtggtcagc ggtgttctcg ccgaacggca agcagctggt
     7021 cacgaccgat gaccggaacg cccagatttg ggacgcgcag tcctaccggc tactgttcac
     7081 gttgaaccac ggtgacatgg tctatcacgc ggtgtacagc gccgatggca cgagggttgt
     7141 caccggctgt ggtgacggcg cggtccggat ctgggacgcc gtgcgcggag tgctccttcg
     7201 cgagtttcgg cgcgacaagg gcaagcttca ctactacgtc gtagctctgt ctcccgacgg
     7261 gaagctcgtg gccggcctcg atctgggagt ggcacatgtt tgggaagtcg acaccggcac
     7321 gccaattgcg gaactgcgag acgccgagcc gtcaagcttt ccatcgctcg cattcagcac
     7381 cgatggccgc tggctcgcaa tgagcactgg tgacgacgta caggtcttcg aggcgaagac
     7441 gtggacggtg gtgcacaaca tttcgggact gggcatccac agcctgagct gggatccgac
     7501 ggggccacat ctcgtgaccg gcagtgcaga gggtgacgcg tcggtctggg taatgccgag
     7561 cggcgcacga gctcatcatc tccgtgaact cggagagtcg atcgacgcgg tagcgttctc
     7621 gcccgacagt cgactaatcg tcgctggtag ccgcgacggt tcggtacaag tctgggacgc
     7681 gacgtccggc aagttgcgaa gccagaacaa ttaccttcgg agcgagatcc tctccgttga
     7741 gttcaatcgg aactcgtcct tgattgcggc atccggctca aacggctctg ttgccgtaat
     7801 ggacgctgca tcggggatgc cggtcacgaa ccttgacgga cctaccacca aggtgcgggt
     7861 tgcgcatttt gatcccagct cgcagcgtgt ggtaggcgct tcctgggatg gcaccgcccg
     7921 ggtctgggat gcgaccgcgc agtaccgcca ttggagcgca ccgccgaaca at
//
LOCUS       DASVOR010001611         1675 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_250642,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001611 DASVOR010000000
VERSION     DASVOR010001611.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_250642"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="VF516_30240"
     CDS             <1..403
                     /locus_tag="VF516_30240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112065.1"
                     /translation="HARRKVRYRRAWLASSGGIRTRKMTLPYFLKDVAPPVPYGPTCP
                     GRTLDIFNRLSLLPSWPWGPIDLCEEASRGAPMSVRRAGGDADHCVIERASMLAHRRS
                     APRRDLASIDDPPAVWPRFVCKLATFGRCCE"
     gene            563..997
                     /locus_tag="VF516_30245"
     CDS             563..997
                     /locus_tag="VF516_30245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112066.1"
                     /translation="MIRLIDESRHREAVVEMIHDAPTRQCPPAALELLERAHCNSMLI
                     DLRSLGDPDALVIEEEVAAPDPGPATLWSEPEAEQATATLLYAPMTKNAMPPIAASAR
                     SIATGARRGRKRVTSVRLLLCLGVLVLLCLGVLVFLDLGTWA"
     gene            complement(1086..>1675)
                     /locus_tag="VF516_30250"
     CDS             complement(1086..>1675)
                     /locus_tag="VF516_30250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112067.1"
                     /translation="RIDQLELDVVYGEHHATTTTQAAGGRTTELPLATAIVLDLPPDL
                     PGGDPVAVGVVAAGKLGASVLGTGAGSTMIAPRHHAAIAIELSPTADCTAGAFYCGGD
                     QLAGDPATLYQCNAGGVPIARGACRYGCVVRPAHDDVCRGGGGTCVDGSRYCGGDKLD
                     GDPQTLYVCMAGAGVSPQRCAAGCVIRPGSDDVCR"
BASE COUNT          249 a          577 c          576 g          273 t
ORIGIN      
        1 gcatgcccgt cgcaaggttc gctaccgacg tgcctggctg gcttcgtcgg gcgggattcg
       61 cacccgcaag atgacgcttc cgtatttctt gaaggacgtc gcacctcctg ttccttacgg
      121 accaacatgt cctggtcgca ccctggacat attcaatcgg ttgagtctgt tgccaagctg
      181 gccttggggc ccgatcgacc tttgcgaaga agcttcgcgc ggcgccccca tgtcggtgcg
      241 gcgggcaggt ggcgacgctg accactgcgt catcgagcgc gcctcaatgc ttgcgcatcg
      301 gcgatctgct cctcgtcgag acctggcgag catcgatgat ccgcctgcag tctggccgcg
      361 atttgtttgc aagctcgcga cgtttggccg ttgctgcgag tgagcttacg gttgggcgtg
      421 agtacgaaac cacgcgatgc gagcttcatc gcgcaaacgt tgcgacgttc tcgacggaga
      481 ttcccaccat cttcccatgg ccgcgagaac gaaccccaca ggcgagtggt gctcaccaac
      541 gcgtgagatc acgaactcgc acttgatccg cctcatcgac gaaagccgcc atcgcgaggc
      601 ggtcgtcgag atgatccacg acgcgccgac ccgccaatgt ccccccgccg cgctggagct
      661 cctcgagcgt gcgcactgca acagcatgct catcgatctc cgatcgctcg gtgatcccga
      721 tgcgctggtg atcgaggagg aggtggccgc gccggatccc ggcccggcga cgctctggtc
      781 ggagcccgag gccgagcaag cgacagcgac gctgctgtat gcaccgatga cgaagaacgc
      841 gatgccgccc atcgcggcgt cggcgcggag catcgcgacc ggcgctcgcc gtggccgcaa
      901 gcgcgtcacc agcgtacgcc tgcttctgtg tctgggcgtg ctcgtgcttc tgtgtctcgg
      961 cgtgctcgtg tttctcgacc tcggcacatg ggcgtgaccg acgccgacgt cgtcgacgca
     1021 tacgtccccg gtcctgatcg cgtccagact ccgcgcggcg gtgagccgca tcggcgcgag
     1081 gcgggtcacc ggcagacgtc gtcgctcccg gggcggatca cgcagcccgc ggcgcagcgc
     1141 tgcggcgaga cgccggcgcc ggccatgcag acgtagagcg tctggggatc gccgtcgagc
     1201 ttgtcgccgc cgcagtagcg gctgccgtcg acgcaggtcc cgccgccgcc gcggcagacg
     1261 tcgtcgtgcg cggggcgaac cacgcagcca tagcggcagg cgccgcgcgc gatcggcacg
     1321 ccgccggcgt tgcactggta gagcgtggcc ggatcgccgg cgagctgatc gccgccgcag
     1381 tagaacgcgc cggcggtgca gtccgccgtc ggcgacagct cgatcgcgat cgcggcgtgg
     1441 tggcgggggg cgatcatcgt cgagccggcg cccgtgccga gcaccgaggc gccgagcttg
     1501 ccggccgcga ccacgccgac cgcgaccgga tcgccgccgg ggagatccgg ggggagatca
     1561 agcacgatcg cggtcgcgag cggcagctcg gtggtgcggc cgcccgccgc ctgggtcgtg
     1621 gtcgtcgcat gatgctcgcc gtacaccacg tcgagctcga gctgatcgat ccgcg
//
LOCUS       DASVOR010001612         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_162402,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001612 DASVOR010000000
VERSION     DASVOR010001612.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162402"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1031..1549
                     /locus_tag="VF516_30255"
     CDS             1031..1549
                     /locus_tag="VF516_30255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112068.1"
                     /translation="MSRARAIRSPRALEAAMFELAFDVEGNPIEVPSAAVSWRVRKQG
                     LRGRPEVVYGRDGLPLIVPIDATLAELRSAVQLEGRYRLDPVDGQRREIAGVRSAYVR
                     LGPPAGPAAACAATSSSVSSDAAETVIRANLDLAKTILGQVELLLGVARGLLRTAEDA
                     SQAIESNRDLVK"
     gene            1572..>2102
                     /locus_tag="VF516_30260"
     CDS             1572..>2102
                     /locus_tag="VF516_30260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112069.1"
                     /translation="MVKTTSSLGSIYRRSDQAMFELAYNVNGEPFEVPPTMAAWRVRK
                     LKPKGAPEVVYGRDGLPLFLPMDADIEDLRREARGDGRYRLDPVDEHNRPVPNAPSGY
                     VCVHPIERAPEPAAPAQPPAAAGQVEITHALVAALLESQRQHTELARMYVSQFPVVSN
                     AMAGMVRTAGDVGLTAR"
BASE COUNT          332 a          713 c          709 g          348 t
ORIGIN      
        1 ctgggtcaca cgtttgccaa tccccgcaag tcgacgcgca gtgaaaattt cgagtctctg
       61 cagcagctcg tcgctgaatt gctgcttgaa ggcttcgctc catggtgatg cgtcgtcgtc
      121 cgggccttcc cgcggtgaac catcgcgtgc gccgtcgtcc gggccttccc gcggcgaact
      181 ctcgtgtgcg ccgtcgtccg gaccttcccg ctgtgaacca tcacgtgaag tcatcatcac
      241 ccgactcgcg tatgccgcca aacgttgcac gtgccgcgag acgcggcaac gagaaacagc
      301 gttgccgcgc acagcgacat cgcgccgcgc gacacccgga acacggtgga acaccgacgg
      361 tactggggca gcgaagtcgc gccgcgggcg tcgcggcccg accgcgctgc gttccgcaca
      421 tgcatcacgt acattcccta catgcatcac acacattccc cgaatgcgtc acgcgtgaag
      481 tcgggcggcg acggcgcagc agccttgcag tgcgtcatcg cgtacgcgtg gcgcacgtga
      541 tcaactgccg acgtttgccg acgggcgcat gccagtagcg tcctgcgtac accaaccgag
      601 cggtccccag tgttcccgga cgcgcctccc gcggtgtcac cggcatcgcg cgcgtgatgt
      661 cgccggttcc cgcaggggcc gacacgccag cggcgaaact cacgcggctg agcaaggccg
      721 atccgccgcg ctggcggtcg ttctgcggca ccgagaaagc cccaatgcgc gcgatggcca
      781 tgtctgcgcg gggtcgtttt cgacgagaaa tagcctctcg cagacggtgc cggagccgcc
      841 tgatcgaggg catctttcgg gcaatccgag ggccttccca cccccctagt ttcggggcag
      901 tccggaccag ttcgcccgaa gtctcccccc aagtccgtgg cagcgtcgcc atggcagcgc
      961 cgtcatggtc cgggcgccgc gtgcggcacg cccacaacgc cggttgccgt cgcggcgagc
     1021 tgtgcctacg gtgtcccggg cgcgcgcgat ccgatcgccg cgcgctttgg aggcggcgat
     1081 gttcgagctt gcgttcgacg tggaaggcaa cccgatcgag gtaccgagcg cggcggtcag
     1141 ctggcgcgtt cgcaagcagg gtctccgcgg gcggccggag gtggtgtacg gccgcgacgg
     1201 attgccgctg atcgtgccga tcgatgccac actcgccgag cttcgtagcg cggtgcagct
     1261 ggagggccgc taccggctgg atccggtcga cgggcagcgg cgggagatcg ccggggtgcg
     1321 gtccgcgtat gtccgcctgg ggcccccggc tgggccggcc gctgcgtgtg ccgcgacctc
     1381 gagctcggtc agctcggacg cagcggagac cgtgatccgc gcaaacctcg atctggcaaa
     1441 aaccatcctg ggccaggtgg agctgctcct gggcgtggcc cgtggtctgc tccgtactgc
     1501 cgaggacgcc agtcaggcga tcgagtcaaa tcgagatctt gtaaaatagt ctgaggaatc
     1561 gacatcgcgc cttggtcaaa acgacctcct cgttaggatc tatctatagg aggtctgatc
     1621 aagcgatgtt tgagctagct tacaatgtga atggcgagcc gttcgaggtg ccgcccacga
     1681 tggccgcctg gcgcgtacgc aagctcaagc ccaagggcgc gcccgaagtc gtctacggcc
     1741 gcgacggctt gccgctgttc ctgccgatgg atgccgacat cgaggatctc cggcgcgagg
     1801 cgcgcggcga tgggcggtac cggctggatc cggtcgatga gcacaaccgg ccggtgccca
     1861 acgcgccatc gggctatgtg tgcgtgcacc cgatcgagcg cgcgcccgag ccggccgcgc
     1921 cggcgcagcc ccctgcagct gcgggtcagg tcgagatcac acacgccctg gtcgctgcgc
     1981 tcctggagtc gcagcggcag cacaccgagc tggcccggat gtacgtctcg cagttcccgg
     2041 tcgtgtcgaa cgcgatggca ggcatggttc gcacggccgg cgacgtgggg ctcaccgcgc
     2101 gt
//
LOCUS       DASVOR010001613         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_424953,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001613 DASVOR010000000
VERSION     DASVOR010001613.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424953"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2145)
                     /locus_tag="VF516_30265"
     CDS             complement(<1..>2145)
                     /locus_tag="VF516_30265"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8112070.1"
                     /translation="AQRLGRGIRFGAIDVAIGHAVMRDVEIRGPLDGDTPLVHIDQID
                     LDFDGWRALFGTVELGAARLDGVLVTIHRDASGQDNVHDVLDRLRAKGEAGGGAPGPR
                     PTSITVTHAKLLANDELTGATGLVADADARWTPDRLVAHARGVTATTLNAPRASAATI
                     DVERVAGATPIVTVDGGEIALWPRLALSGIAGRVVANPKRAGEYVIDLAGGYGGVPGR
                     LWTARGALDPEALTASFDLEAAKFQLDRLAPILEHSAVVDYATTSVDTRLHLDVDRVG
                     AKFSGSFHLTGLNVGHPLIAEHEVRDLDLSGQIAGSFERATRKLELTQGDFVVRNMPF
                     SITGVVTAPHRAAVAEAVAVDADKAGREPPRVGPHGIEVLKLRLVIPPIDCQRVLNAI
                     PTEMAPYLAGYKIKGVFDTDVHVDIDWSDLDATQLDGHIGIKHCKVVDEPVDSPKRLE
                     QEFEQTVEVEQGEWESFVVGPSNDEFVPLDQISPYLIKSIMSTEDSAFYQHHGFITSE
                     FRTALVNNLKAGKFVQGASSITMQMVKNVLLYREKTLARKLQELFLTWHVENTLTKDR
                     ILEIYLNVIEYGPGLYGIGPAAYHFFGKKAKDLNPTEAAFFSTILPSPKERYKQYCAG
                     TLTRWTEGKIDRILQIMLKRDRLTQAEYDTAIATPLLFSKDGMESEEDCMKRVKKAIK
                     NARPTNPLAAQASKDGDPKRDKHKPDPRDHHDR"
BASE COUNT          285 a          748 c          738 g          374 t
ORIGIN      
        1 cgatcgtggt ggtcgcgcgg atccggcttg tgcttgtcgc gcttggggtc gccgtccttg
       61 ctggcctgcg cggccagcgg gttggtcggc cgggcgttct tgatcgcctt cttcacccgc
      121 ttcatgcagt cctcctcgct ctccatcccg tccttggaga acaagagcgg cgtggcgatc
      181 gcggtgtcgt actcggcctg ggtcaggcga tcgcgcttga gcatgatctg caggatccgg
      241 tcgatcttgc cctcggtcca tctggtcagc gtgcccgcgc agtactgctt gtagcgctcc
      301 ttgggcgacg gcaggatcgt cgagaagaac gccgcctcgg tcgggttcag gtccttggcc
      361 ttcttgccga agaagtgata ggcggccggc ccgataccgt acagcccggg gccgtactcg
      421 atcacgttga ggtagatctc gaggatccgg tccttggtca gcgtgttctc gacgtgccag
      481 gtcaggaaca gctcctggag cttccgcgcc agcgtcttct cgcgatacag cagcacgttc
      541 ttgaccatct gcatcgtgat cgacgacgcg ccctgcacga acttgccggc cttgaggttg
      601 ttgaccagcg cggtgcggaa ctcgctcgtg atgaagccgt ggtgctggta gaacgccgag
      661 tcctcggtcg acatgatcga cttgatgagg tacggcgaga tctggtcgag cggcacgaac
      721 tcgtcgttgc tcggcccgac cacgaacgac tcccactcgc cctgctcgac ctcgacggtc
      781 tgctcgaact cctgctccag ccgcttgggc gagtccaccg gctcgtcgac caccttgcag
      841 tgcttgatcc cgatgtggcc gtcgagctga gtcgcgtcga gatcgctcca gtcgatgtcg
      901 acgtggacgt cggtgtcgaa cacccccttg atcttgtagc cggccaggta cggcgccatc
      961 tcggtcggaa tggcgttgag cacgcgctgg cagtcgatcg gcgggatcac gaggcggagc
     1021 ttgagcacct cgatcccgtg cggcccgacc cgcggtggct cgcggcccgc cttgtcggcg
     1081 tcgaccgcga cggcctcggc gaccgcggcc cggtgcggcg cggtcaccac gccggtgatc
     1141 gagaacggca tgttgcgcac gacgaagtcg ccctgggtca gctcgagctt gcgggtggcg
     1201 cgctcgaagc tgcccgcgat ctggcccgac aggtcgaggt cgcggacctc gtgctcggcg
     1261 atcagcggat ggccgacgtt gagcccggtc aggtggaagc tgccgctgaa cttcgcgccg
     1321 acccggtcga cgtcgaggtg cagccgggtg tcgaccgagg tcgtggcgta atcgaccacc
     1381 gccgagtgct cgaggatcgg cgcgagccgg tcgagctgga acttcgccgc ctcgaggtcg
     1441 aacgacgcgg tcagcgcctc gggatcgagc gcgcccctgg cggtccacag ccggcccggc
     1501 acgccgccgt agccgcccgc caggtcgatc acgtactcgc cggcgcgctt cgggttcgcg
     1561 acgacccggc ccgcgatccc cgacagcgcg agcctgggcc acagcgcgat ctcgccgccg
     1621 tcgaccgtca cgatcggcgt cgcgccggcg acccgctcga cgtcgatcgt cgccgccgac
     1681 gcccggggcg cgttgagcgt ggtcgcggtg acgccgcggg cgtgcgcgac cagccggtcc
     1741 ggcgtccacc gcgcatcggc gtcggcgacc agcccggtcg ccccggtcag ctcgtcgttg
     1801 gcgagcagct tggcgtgggt caccgtgatc gacgtcggcc gcggcccggg cgcaccgccg
     1861 cccgcctcgc cctttgcgcg caggcggtcg agcacgtcgt gcacgttgtc ctgcccgctg
     1921 gcgtcgcggt ggatcgtcac cagcacgcca tcgagccgcg ccgcgccgag ctcgaccgtg
     1981 ccgaacagcg ctcgccagcc gtcgaagtcg aggtcgatct gatcgatgtg caccagcggt
     2041 gtgtcgccgt cgagcggacc gcggatctcg acgtctcgca tcaccgcgtg cccgatcgcg
     2101 acgtcgatcg cgccgaaccg gatgccgcgg ccgagccgct gcgcc
//
LOCUS       DASVOR010001614         2603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_454698,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001614 DASVOR010000000
VERSION     DASVOR010001614.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454698"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="VF516_30270"
     CDS             <1..1701
                     /locus_tag="VF516_30270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112071.1"
                     /translation="WRPVLGLGAFGAAAVAIATLAWSGRSQPVPYHLEQLTHRTDLKR
                     ATISPDGTRLAFVRGDALVIRGTEPDTEERVVVDHGIADIPISWSPDGTHLLAAAQSE
                     SAPWTEAVLVDAAGGVQFKIPALGRASFLSSTEVAATTVRRHSIDIYSLASHSAPVTT
                     CSVPGDYAFLWSFVGLPDGTLVTEIVKGDSRSLVILRRDCRVRATFTGERISSIATTD
                     TGTIVALVSGVGSSDILEISLDGMVLSRRRISGDVDELLGRRRGIDYVATIALETHLN
                     RVHGTEPPVRQLSVHGGASFSLAPDGATVAWVESGNRGHGRLRLSTLQNLARRGTPLL
                     DNALFAGWSPDGRSLAVLLDQGPDAEVEVIVIDRTGEVQRRLPLRHLDRMAAPVWLDD
                     HRIAAQAEDRTTYRWFDLVTGEEGDLVDRQHGSTYWLARSPRDGTLAMWRNGLPDPHT
                     PEHLWVQAPGRPARPLPVREAIGHYLLPSWSPTGELIVRALDTGEVSRVALDTGELTP
                     IARLPGPSIRSSFDDHVMTLADGDLLGVELELSLSVSAVLADDAPRSHPRREPDPGQL
                     "
     gene            1812..>2603
                     /locus_tag="VF516_30275"
     CDS             1812..>2603
                     /locus_tag="VF516_30275"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112072.1"
                     /translation="MTTAPHAVHSAATLTALYDMGHGPPPAGPSRVGRYQIGDPLGAG
                     AMGVVYRAHDPDLGRDVAIKLVRGNAATPSNVRLLREAQAMARLHHPNVVPIFDVGQA
                     GDAVFVTMPLIEGGTLKRWLRGGARSFDEILDRFLAAGRGLAAAHAAGLVHRDFKPDN
                     VLLGARGEIHVADFGLARLPDDEARDATRPLSIDEPAPEPSVDRLAPGLTQTGTVLGT
                     PAYMAPEQLRGQPIDARADQFSFCVALWEGVYGQRPFPDPPDDAAP"
BASE COUNT          332 a          977 c          932 g          362 t
ORIGIN      
        1 tggcggcccg tcctgggcct gggggcgttc ggcgcggcgg cagtggcgat cgcgacgctc
       61 gcgtggtcgg ggcgatcgca gcccgtgccg taccacctgg agcagctgac ccaccgcacg
      121 gatctcaagc gcgcgacgat ctcgccggac ggcacccggc tcgcgttcgt ccggggcgac
      181 gcgctcgtga tccgcggcac cgagcccgac accgaggaac gcgtggtcgt cgaccacggg
      241 atcgccgaca tcccgatctc gtggtcgccg gacggcacgc acctgctggc ggcggcccag
      301 tccgagagcg cgccgtggac cgaggcggtg ctggtcgacg ccgccggcgg cgtgcagttc
      361 aagatcccgg cgctcggccg ggcgtcgttc ctgtcgagca ccgaggtcgc cgcgacgacc
      421 gtccgccggc actcgatcga catctactcg ctggcgtcgc actccgcgcc ggtcacgacc
      481 tgctcggtgc ccggcgacta cgcgttcctg tggagcttcg tcggcctgcc ggacggcacc
      541 ctggtcaccg agatcgtgaa gggcgacagc cgcagcctgg tcatcctgcg ccgcgactgc
      601 cgggtccgcg cgacgttcac cggcgagcgg atctcgagca tcgccaccac ggataccggg
      661 acgatcgtcg cgctggtctc cggcgtgggg tcgagcgaca tcctggagat ctcgctcgat
      721 ggcatggtgc tgtcgcggcg ccggatcagc ggcgacgtcg acgagctgct cggccgccgc
      781 cgcggcatcg actacgtcgc gacgatcgcg ctcgagaccc acctcaatcg agtacacggc
      841 accgagccgc cggtgcggca gctgtcggtc cacggcggcg cgtcgttcag cctggcgccg
      901 gacggcgcca ccgtggcgtg ggtcgagagc gggaaccggg ggcacggccg gctgcggctg
      961 tcgaccctgc agaacctggc acggcgcggc acgccgctgc tcgacaacgc gctgttcgcc
     1021 ggctggtcgc ccgacggtcg gtcgctggcg gtgctgctcg atcagggccc ggacgccgag
     1081 gtcgaggtca tcgtcatcga ccgcaccggg gaggtgcagc gccgcctgcc gctccgccac
     1141 ctcgaccgca tggccgcgcc ggtctggctg gacgatcacc ggatcgccgc gcaggccgag
     1201 gaccgcacca cgtaccgctg gttcgatctg gtcaccggcg aggaaggcga cctcgtcgat
     1261 cgccagcacg gctcgaccta ctggctggcc cgctcgccgc gcgacggcac gctcgcgatg
     1321 tggcgcaacg gcctgcccga cccacacacc cccgagcacc tgtgggtgca ggccccgggt
     1381 cggccggcgc gcccgctgcc ggtccgcgag gcgatcggac actacctgct gccgagctgg
     1441 tcgccgaccg gcgagctgat cgtgcgcgcg ctggacaccg gcgaggtatc gcgcgtcgcg
     1501 ctggacaccg gcgagctgac cccgatcgcg cgcctgcccg ggccctcgat ccggagctcg
     1561 tttgacgacc acgtcatgac cctggcagac ggcgatctgc tcggggtcga gctcgagctc
     1621 agcctctcgg tatcggcggt cctggccgac gacgcgccgc gctcccaccc gcgccgcgag
     1681 cccgatccgg gccagctgtg aggcggatcg cccggatcgg cgcccggacc catgcagctc
     1741 cgtgatgccg cgggaatttt tcgttttctc ctgccaatct tgatcttgta tcgtgtctga
     1801 ggatggggac ggtgaccacc gcgccacacg ccgtgcatag cgccgccacg ctgacggccc
     1861 tctacgacat ggggcacggt ccaccgccgg ccgggccgtc gcgcgtcggg cgctaccaga
     1921 tcggcgatcc gctcggcgcc ggggcgatgg gtgtggtcta ccgcgcgcac gatccggacc
     1981 tcggccgcga cgtcgcgatc aagctggtcc gcggcaacgc ggcgaccccg tcgaacgtcc
     2041 gcctgctgcg cgaggcccag gcgatggcgc ggctccacca ccccaacgtc gtgccgatct
     2101 tcgatgtcgg ccaggccggc gacgcggtgt tcgtgacgat gccgctgatc gagggcggca
     2161 cgctgaagcg ctggctgcgc ggcggggcgc ggtcgttcga cgagatcctc gaccggttcc
     2221 tcgcggcggg ccgcggcctg gccgccgcgc atgccgccgg gctggtccac cgcgacttca
     2281 agcccgacaa cgtgctgctc ggcgcccgcg gtgagatcca cgtcgcggac ttcggcctgg
     2341 cgcggcttcc cgatgacgag gcgcgcgacg cgacgcgccc cctgtcgatc gacgagccgg
     2401 cgcccgagcc gagcgtggac cggctggccc cggggctcac ccagaccggc acggtgctcg
     2461 gcacgccggc gtacatggcg cccgagcagc tgcgcggcca gccgatcgac gcgcgcgccg
     2521 accagttcag cttctgcgtc gcgctgtggg agggcgtcta tggccagcgg ccgttccccg
     2581 acccgcccga cgatgccgcc ccg
//
LOCUS       DASVOR010001615         2206 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_454711,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001615 DASVOR010000000
VERSION     DASVOR010001615.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2206)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2206)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2206
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454711"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..410
                     /locus_tag="VF516_30280"
     CDS             114..410
                     /locus_tag="VF516_30280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112073.1"
                     /translation="MSDLTAKEQKAVRTALRFLRLRVGAWKPLVKALRYEYDSIQKVA
                     TGKKAVTPALALRVARFVGVPMDELLAGQWLSPRVCPHCGHPPDDFQDEDTIAG"
     gene            455..640
                     /locus_tag="VF516_30285"
     CDS             455..640
                     /locus_tag="VF516_30285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112074.1"
                     /translation="MNDSAASKAPADPFVVEHRTDEILLKLLDGIERDVTDDKLPSPR
                     VEAFEALCRAVRARSGR"
     gene            complement(629..1006)
                     /locus_tag="VF516_30290"
     CDS             complement(629..1006)
                     /locus_tag="VF516_30290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112075.1"
                     /translation="MAEVRTLVRDPAERRTTEALHLWSFPRRKKVQRASKKVQPARDL
                     PAQPWLRYFRGMSSYLDSIPSAVATIVSSCYGSLLAGVGKGPSFQKEMEKHVIDFTDQ
                     LTRTIAKALADAEAGEPSGKSAT"
     gene            1140..1988
                     /locus_tag="VF516_30295"
     CDS             1140..1988
                     /locus_tag="VF516_30295"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8112076.1"
                     /translation="MPSVASRVDSDLSGLDDRLLEPKARYEMYDGELVYVSPADPPHG
                     ERHLQLAALIEAHTGFEFEAAADLLTRTSKVDDVAPDVSVYPAAPDPRTGGRQLEQLA
                     FEIISAQSLGRATTKAVKLTARGVRRVLAIDVERSRVLEWSIDRGTWHELDAAGHIAD
                     PALEVPLPIEAMVHAAKADDAVARALVARHNLVIEAVRAEDRAAGKIEGKIEGKIEGK
                     IEGKIEGKIEGKIEALLAILAARHIGPDSAARERILRECDPQRLDRWITRAAISATLA
                     EVLDSD"
BASE COUNT          358 a          760 c          733 g          355 t
ORIGIN      
        1 gtcgcgcaca acctgtgttg cctcgtcaag gccatcttca ccgcagggct tgcgcccgcg
       61 ttctggcagg acgcgcccgc cgccctgcct gccccgcttt ccgaggagca agcatgagcg
      121 atctgacagc gaaggaacag aaggccgtcc gcaccgcgct gcggttcctg cggctgcgtg
      181 tcggcgcgtg gaagccgctg gtgaaggctc tgcgctacga gtacgacagc atccagaagg
      241 tcgccaccgg caagaaggcc gtcaccccgg cgctcgcgct gcgcgtcgcc cggttcgttg
      301 gcgtgccgat ggacgaactg ctcgccggcc agtggctctc gccccgggtc tgcccgcact
      361 gcggccaccc gccggatgat tttcaggacg aggacacgat cgccggttga cgggtgggtt
      421 gacgtcgagc ggaggccatg atccatctgg cctcatgaac gacagcgcag ccagtaaagc
      481 gcccgccgat ccgttcgtgg tcgaacatcg gaccgacgag atcctactca agctcctcga
      541 tgggatcgag cgcgacgtca ccgacgacaa gctgccgagt ccgcgcgtgg aggcgttcga
      601 ggcgctgtgt agagccgtgc gcgcgcggtc aggtcgctga cttgcccgac ggttccccgg
      661 cttccgcgtc agcgagggcc ttcgcgatcg tcctcgtcaa ctggtcggtg aagtcgatga
      721 cgtgcttctc catctccttc tgaaagctcg ggcctttgcc cacgccggct agcaacgagc
      781 catagcaaga gctgacgatg gtagcgacag ccgatgggat cgagtcgagg tagctgctca
      841 tgccgcgaaa gtacctcagc catggctggg cgggcagatc cctggccggc tgcacctttt
      901 tcgaggcccg ctgcaccttt tttcgacgcg ggaagctcca aaggtgcaaa gcctcggttg
      961 tccggcgttc ggccggatct cggacaagcg tccgaacttc ggccattccc cggacagcgt
     1021 cgactgcgat ctcgccgcgt tggccgtggc gcggcgcttg ctctcgagcc gcgcatgatc
     1081 tcgccgtatg ctctgtggat cctgctccgc ggcgtgcacc gtcggaggta tgctgggaca
     1141 tgccgtcggt tgcctcgcgc gttgactccg acctgtcggg cctcgacgat cgtctcctcg
     1201 agcccaaggc tcgctacgag atgtacgacg gggagctagt ctacgtgtcc cccgccgatc
     1261 caccccacgg tgagcgccac ctgcagctcg ctgcgttgat cgaagcccac accgggttcg
     1321 agttcgaggc cgcggccgat ctgctgaccc ggacctccaa ggtcgatgac gtcgcgcccg
     1381 atgtcagcgt gtaccccgct gccccggatc ccaggacggg cggccgccag ctcgaacagc
     1441 tcgcgttcga gatcatcagc gcccagtcgc tcggccgcgc caccaccaag gcggtcaagc
     1501 tcaccgcgcg tggcgtgcgc cgcgtgctcg cgatcgacgt cgagcgatcg cgcgtcctgg
     1561 agtggtcgat cgatcgcggc acctggcacg aactcgacgc cgcgggccat atcgcagacc
     1621 ccgcgctcga ggtgccgctg cccatcgagg ccatggttca cgccgccaag gccgacgacg
     1681 cggtcgcccg cgccctggtg gccaggcaca accttgtcat cgaggcggtc cgggcggagg
     1741 atcgagctgc cggcaagatc gagggcaaga tcgagggcaa gatcgagggc aagatcgagg
     1801 gcaagatcga gggcaagatc gagggcaaga tcgaggcgct gctcgcaatc ctcgccgccc
     1861 gccacatcgg tccggacagc gccgcgcgcg agcggatcct ccgcgaatgc gatccacagc
     1921 gactcgatcg ctggattacc cgcgcggcga tctcggcgac cctcgccgag gtactcgaca
     1981 gcgactgatc ggtgagatga cgctgcgggt gcggcacacg agctccgcga agcccttgcg
     2041 gcgctcgagg gccgggcgcg ccgtggtggc caagtatgcc aggcgttccg cccccgaaca
     2101 acgaagccac gcgcgggcgc gcggtgttac cctcgggctg cgtgaaccgc acggtcgacg
     2161 acgtctccag catcaccgag gacgcggcgc cggacccggc gatcgt
//
LOCUS       DASVOR010001616         1688 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_17607,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001616 DASVOR010000000
VERSION     DASVOR010001616.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1688
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17607"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1665
                     /locus_tag="VF516_30300"
     CDS             145..1665
                     /locus_tag="VF516_30300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112077.1"
                     /translation="MPVPAERRRSFLARQVATMALSHVQTDTPMALVRAAAWSNSLMR
                     MGVHLPLFLIHDVGVMLSMSRGTGGYALRPREAQLARINVPPGAKPQLDQYRRLLENI
                     AASEVTERLAGLRLRDEMVAVLLSRILGDTYNRWRDRSKAAGAEPLPLDLGILGEIDY
                     ADHFRDFDPRGLWGFVEHLVAQSMHIYTQVELIDLDTVRLLGLFKEDSASGSEALGGA
                     IDLVDLFAALGSPEANDVANFSLDLLPSVLETRRSTGAQTFAVDGYASIERKGNIDSL
                     VLSELAYDREIFEQKVVDAELLYYGRDKQREDERRLQYLLIDCSASMRGQRQVFARGL
                     ALALIKKLTLEGDEIWVRFFDSRLHETIKIGRSGQVPVPYLLSFRSERGRNYSKVFRQ
                     LLVEVTRLRREQKRRVVLYTITHGQCHIEPELVTALKQQAFLYGVVVLPSSDHLPEFV
                     PLLDRSQIVSGEALTNRAERQRRALDIVGDASAAPKGPKPTTTAPSASMRNMPAMK"
BASE COUNT          244 a          621 c          572 g          251 t
ORIGIN      
        1 cggttgcgtc gtacaggttc atggctccga cgctaccgca aaacctctcg cagccgccgg
       61 cgctcccgat cccccgatcg ggtgggtcga gccggggcgc ggcggcgcgc cgctcctgcg
      121 ggcgcaccgt ggtagcttga ccgcgtgccg gtccccgccg agcggcgtcg cagcttcctc
      181 gcgcgccagg tcgcgacgat ggcgctgtcg cacgtccaga ccgacacccc gatggcgctg
      241 gtccgcgccg ccgcgtggtc gaactcgctg atgcggatgg gcgtgcacct gccgctgttc
      301 ctgatccacg acgtcggcgt catgctgtcg atgtcgcgcg gcaccggcgg ctacgcgctc
      361 cggccgcgcg aggcccagct ggcgcggatc aacgtgccgc cgggcgccaa gccgcagctc
      421 gaccagtacc gccggctgct cgagaacatc gccgccagcg aggtcaccga gcggctcgcc
      481 gggctgcggc tgcgcgacga gatggtcgcc gtgctgctgt cgcggatcct cggcgacacc
      541 tacaaccgct ggcgcgaccg cagcaaggcc gccggtgccg agccgctgcc gctcgacctc
      601 gggatcctgg gcgagatcga ctacgccgac cacttccgcg acttcgaccc gcgcggcctg
      661 tgggggttcg tcgagcacct ggtcgcgcag tcgatgcaca tctacaccca ggtcgagctg
      721 atcgacctcg acaccgtgcg gctgctcggc ctgttcaagg aggacagcgc gtcgggctcc
      781 gaggcgctcg gcggcgcgat cgacctcgtc gacctgttcg ccgcgctggg ctcgcccgag
      841 gccaacgacg tcgccaactt ctcgctcgac ctcctgccca gcgtgctcga gacccggcgc
      901 tcgaccggcg cgcagacctt cgcggtcgac ggctacgcgt cgatcgagcg caagggcaac
      961 atcgactcgc tggtgctgtc cgagctcgcc tacgaccgcg agatcttcga gcagaaggtg
     1021 gtcgacgccg agctgctgta ctacggccgc gacaagcagc gcgaggacga gcgccggctg
     1081 cagtacctcc tgatcgactg ctcggcgtcg atgcgcggcc agcgccaggt gttcgcccgc
     1141 ggcctggcgc tcgcgctgat caagaagctc acgctggagg gcgacgagat ctgggtccgg
     1201 ttcttcgact cgcggctgca cgagacgatc aagatcggcc ggtccggcca ggtcccggtg
     1261 ccctacctcc tgtcgttccg cagcgagcgc ggccgcaact acagcaaggt gttccgccag
     1321 ctcctggtcg aggtcacccg gctgcgccgc gagcagaagc gccgggtcgt gctctacacg
     1381 atcacccacg gccagtgcca catcgagccc gagctggtga ccgcgctcaa gcagcaggcg
     1441 ttcctgtacg gcgtcgtcgt gctgccgtcg agcgatcacc tcccggagtt cgtgccgctg
     1501 ctcgatcgct cgcagatcgt gtcgggcgag gcgctgacca accgcgccga gcgccagcgc
     1561 cgcgcgctcg acatcgtcgg tgacgcgagt gccgcgccga agggtccgaa gcccaccacc
     1621 acggcgccga gcgcgagcat gcgcaacatg ccggcgatga agtaatggcg cgtcgcacgt
     1681 tcaactcg
//
LOCUS       DASVOR010001617         2663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_74839,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001617 DASVOR010000000
VERSION     DASVOR010001617.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74839"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..651
                     /locus_tag="VF516_30305"
     CDS             100..651
                     /locus_tag="VF516_30305"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8112078.1"
                     /translation="MDHAYDGIREYDNPLPGWWSAVFAATFVFAFLYFAYYNIAHWGR
                     TPDEHYKIALAGWQASYKGGPAGGGINVTEDMLAAGAQNPDVVAQGAAVFAARCVGCH
                     AEGGRGQIGPNLTDLYQIHGTTRMDLYATITGGAPGTAMIAWGEVMRPTEIVAVATYV
                     SSLRGKNLSGKEPQGKPVPALAR"
     gene            689..2095
                     /gene="ccoG"
                     /locus_tag="VF516_30310"
     CDS             689..2095
                     /gene="ccoG"
                     /locus_tag="VF516_30310"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEX8112079.1"
                     /translation="MTPPLSKERVLSTLNQDGTRNWIRPRLARGRFLRRRRIVGYALI
                     ALFVALPLIRIHGRPALLIDLVARELSAFGTVFRPSNGVILMLLGLTIALAVFVLTAL
                     FGRVWCGWGCPHTVYLEHVFRPIERWLEGTPAQQARLPRLHPRKLIKWAIYAALAFAI
                     ANVFLAYFVGVARLRLWVLESPAEHPGGFAVVLAVTALMLGNFGWFREQTCIVACPYG
                     RLQSVLLDRQSLIIGYDARRGEPRTPLKKHLPAADGRPGECVDCGACVAVCPTGIDIR
                     DGLQMECVGCAQCIDACDAVMDKLHRRRGMIGYTSQDQLAGKPRRLLRPRLLLYPALL
                     TVVGTLFAWSVASRPASEVTALRIDGPSFVQLPDGRIASAVQLKIENETDQSRHYAIS
                     LAGTPDAVLKSPLAVWEIPAHHARTIPLYVEVVAATFQHGQRRVYLRIFDDEGFERIV
                     PATLLGPDQPTGLPERVQ"
     gene            2092..2541
                     /locus_tag="VF516_30315"
     CDS             2092..2541
                     /locus_tag="VF516_30315"
                     /inference="COORDINATES: protein motif:HMM:NF017559.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEX8112080.1"
                     /translation="MTPRLKWLLAIAGLLVGNVIAMVILAVVAGNGRTQVIPAYYDRA
                     AHFDDEIARASASRALGWHAEVAIAAGAIDVTVSDAAGHAVDDARVRVTGYQRAHAGE
                     PVDVELVRLGGGHYRAGLRERRGWHDLTVAADRGDAHYQQRVAVEAR"
BASE COUNT          355 a          943 c          978 g          387 t
ORIGIN      
        1 gcagggccgc gcgacgcgac gcgcggaggg gtgaatcggc cggctcagcc ggccgagagg
       61 gggcgggaat gccccctctg gatcgacgag tcgcggctga tggatcacgc gtacgacggg
      121 atccgcgagt acgacaaccc gttgccgggc tggtggagcg cggtgttcgc cgcaacgttc
      181 gtgttcgcct tcctgtactt cgcgtactac aacatcgcgc actggggccg gactccggac
      241 gagcattaca agatcgcgct cgcgggctgg caggccagct acaagggcgg gccggccggc
      301 ggcggcatca acgtcaccga ggacatgctc gccgcgggcg cccagaaccc cgacgtcgtc
      361 gcgcagggcg ccgcggtgtt cgccgcgcgc tgcgtcggct gccacgccga gggcggccgc
      421 ggccagatcg gcccgaacct caccgacctg taccagatcc acggcacgac ccggatggac
      481 ctctacgcga cgatcacggg cggcgccccg ggcaccgcga tgatcgcgtg gggcgaggtc
      541 atgaggccga ccgagatcgt cgcggtggcg acctacgtgt cgtcgctgcg cggcaagaac
      601 ctctccggca aggagcccca gggcaagccg gtgccggcgc tggccaggtg acgggatcga
      661 tgatccgtat tccagacagg aggcgaccat gacaccgcca ctttccaagg agcgcgtgct
      721 gtcgacgctc aaccaggacg gtacgcgcaa ctggatccga ccccggctgg cgcgcggccg
      781 gttcctgcgc cggcgccgga tcgtcgggta cgcgctgatc gcgctgttcg tcgcgctgcc
      841 gctgatccgg atccacgggc ggccggcgct gctgatcgac ctcgtcgcgc gcgagctgtc
      901 ggcgttcggc acggtgttcc ggccgagcaa cggcgtcatc ctgatgctgc tcggcctgac
      961 catcgcgctg gcggtgttcg tgctgaccgc gctgttcggc cgggtgtggt gcggctgggg
     1021 gtgcccgcac acggtgtacc tcgagcacgt gttccggccg atcgagcgct ggctcgaggg
     1081 cacgccggcg cagcaggcca ggctgccgcg gctacatccc cgcaagctga tcaagtgggc
     1141 gatctacgcc gcgctggcgt tcgcgatcgc caacgtgttc ctggcgtact tcgtcggcgt
     1201 cgcgcggctg cgcctgtggg tgctggagtc gccggcggag cacccgggcg ggttcgccgt
     1261 ggtgctcgcg gtcaccgcgc tgatgctcgg caacttcggc tggttccgcg agcagacctg
     1321 catcgtggcc tgcccgtacg gccggctgca gtcggtgctg ctcgatcgcc agtcgctgat
     1381 catcggctac gacgcccgcc gcggcgagcc gcggacgccg ctcaagaagc acctgccggc
     1441 ggcggacggc cgcccgggcg aatgcgtcga ctgcggcgcg tgcgtcgcgg tgtgcccgac
     1501 cgggatcgac atccgcgacg gcctgcagat ggagtgcgtc ggctgcgcgc agtgcatcga
     1561 cgcctgcgac gcggtgatgg acaagctgca ccggcggcgc ggcatgatcg gctacacctc
     1621 gcaggaccag ctcgccggca agccgcgcag gctgctgcgg ccgcggctgc tgctctatcc
     1681 ggcgctgctc acggtggtcg ggacgctgtt cgcgtggagc gtggcgtcgc ggccggcctc
     1741 cgaggtcacc gcgctgcgca tcgacgggcc gagcttcgtg cagctgcccg acggccggat
     1801 cgcgtcggcc gtgcagctca agatcgagaa cgagaccgac cagtcgcgcc actacgcgat
     1861 ctcgctcgcc gggacgcccg acgcggtgct gaagtcgccg ctcgcggtgt gggagatccc
     1921 ggcgcaccac gcccggacca tcccgctgta cgtcgaggtc gtggccgcga cgttccagca
     1981 cggccagcgc cgggtctacc tgcggatctt cgacgacgag ggcttcgagc ggatcgtgcc
     2041 ggccacgctg ctcggccccg accagccgac cgggctgccg gagcgcgtgc aatgacgccg
     2101 cggctcaagt ggctgctcgc catcgccggg ctgctggtcg gcaacgtgat cgcgatggtg
     2161 atcctcgcgg tggtcgccgg caacggccgg acgcaggtga tcccggcgta ctacgaccgc
     2221 gccgcgcact tcgacgacga gatcgcccgt gcatcggcca gccgcgcgct gggctggcac
     2281 gccgaggtcg cgatcgcggc cggcgcgatc gacgtgacgg tgtcggacgc cgccggccac
     2341 gcggtcgacg acgcgcgggt ccgcgtcacc ggctaccagc gcgcccacgc cggcgagccg
     2401 gtcgacgtcg agctcgtccg gctcggcggt gggcactacc gcgccggcct ccgcgagcgc
     2461 cggggctggc atgacctcac cgtggccgcc gatcgcggcg acgctcacta ccagcagcgc
     2521 gtcgcggtcg aggctcgctg acacgccatg atggcactct ggatcacgat cctgggctcg
     2581 agcctcgccg gcagcgtgca ttgcatcgcg atgtgcgggc cgctgatcgg gctgtcggcc
     2641 ggcaggcagt ccgtctcgct cgc
//
LOCUS       DASVOR010001618         2224 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_162455,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001618 DASVOR010000000
VERSION     DASVOR010001618.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162455"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1774
                     /locus_tag="VF516_30320"
     CDS             <1..1774
                     /locus_tag="VF516_30320"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8112081.1"
                     /translation="NKLTAWLRSDTLAANPSRAIDLDIQISIDGMRVEAALSDGERSV
                     PIDAAMNAWRAGLDLVLLNGGVLGRLPRAWFDQHGERLADLLAARGDDHRVPIYALPD
                     LAKLCAELEEPPPPELDRLRPLLGEFTGIPAVRAEGGFAGELRPYQQRGLDWLVFCRD
                     AGLGCVLADDMGLGKTIQALAAIRGKTLVVSPTSVLFNWLAEATRFRPDLRIATYRGA
                     RRTLETGADVVLTSYPILRNDTDVLAGVTWDTVILDESQTIKNPDSQVARAAYRLKAR
                     WRLTLSGTPIENRLDELWSQLHFTNPGLLGGRTDFQERWAEPISLGDAAAAARLRERI
                     RPFVLRRMKSEVARDLPPRTDAIMYVELDEAERITYDAIRAATQSEIVALLQAGGGVM
                     AALEALLRLRQAACHPALLPGGVRGGPAPQQSSKLQRLLEALDDVVADGHRALVFSQW
                     TSLLDLIEPHLQAAGIAFTRLDGTTTDRAGVVACFQADGGPPVMLLSLKAGGTGLNLT
                     AADHVFLIDPWWNPAVEDQAADRAHRIGQDKPVMVYRMVARDTVEERILELQDRKRAL
                     ADAALAEAGSAAAITRDDLLALLA"
     gene            complement(1790..2122)
                     /locus_tag="VF516_30325"
     CDS             complement(1790..2122)
                     /locus_tag="VF516_30325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112082.1"
                     /translation="MPQADEVSPERLVELLPPRIWYLTSNGQDMWSRRPYGFLFSTGQ
                     AAEAFARAMGTGEQLFAIGLDAGALISDEMLAGLRNTAVTRLFIDPAIDPDSGDVHGQ
                     ILRLAPLT"
BASE COUNT          297 a          877 c          769 g          281 t
ORIGIN      
        1 caacaagctc accgcgtggc tgcgcagcga cacgctcgcc gccaatccgt cgcgggcgat
       61 cgatctcgac atccagatct cgatcgacgg catgcgggtc gaggccgcgc tgtcggacgg
      121 cgagcgcagc gtgccgatcg atgccgcgat gaacgcgtgg cgcgcgggcc tcgatctggt
      181 cctgctcaac ggcggcgtgc tgggccggct gcccagggcg tggttcgatc agcacggcga
      241 gcggctcgcc gacctgctgg cggcccgcgg cgacgaccac cgcgtgccga tctacgccct
      301 gcccgacctc gccaagctgt gcgccgagct cgaggagccc ccgccgcccg agctcgatcg
      361 cctgcggccg ctgctcggcg agttcaccgg catcccggcg gtgcgcgccg agggcggctt
      421 cgccggcgag ctccggccct accagcagcg cgggctcgac tggctggtgt tctgccgcga
      481 cgccggcctg ggctgcgtgc tcgccgacga catgggcctg ggcaagacga tccaggcgct
      541 cgccgcgatc cgcggcaaga ccctcgtcgt gtcgccgacc agcgtgctgt tcaactggct
      601 cgccgaggcc acccggttcc ggcccgacct gcggatcgcg acctaccgcg gcgcgcgccg
      661 cacgctcgag accggcgccg acgtcgtgct gaccagctac ccgatcctgc gcaacgacac
      721 cgacgtgctc gccggcgtca cctgggacac cgtcatcctc gacgagtcgc agacgatcaa
      781 gaaccccgac agccaggtcg cccgcgcggc ctaccggctc aaggcgcgct ggcggctgac
      841 cctgtccggc acgccgatcg agaaccggct cgacgagctg tggagccagc tccacttcac
      901 caaccccggc ctgctcggcg gccgcaccga cttccaggag cgctgggccg agccgatctc
      961 gctcggcgac gccgccgcgg ccgcccggct gcgcgagcgg atccgcccgt tcgtgctgcg
     1021 ccggatgaag tccgaggtcg cgcgcgacct gccgccgcgc accgacgcga tcatgtacgt
     1081 cgagctcgac gaggccgagc gcatcaccta cgacgcgatc cgcgccgcga cccagagcga
     1141 gatcgtcgcc ctgctgcagg ccggcggcgg cgtgatggcg gcgctcgagg ccctgctccg
     1201 gctccgccag gcggcctgcc acccggcgct gctccccggc ggggtgcgcg gcggcccggc
     1261 gccccagcag tcgtccaagc tccagcgcct gctcgaggcg ctcgacgacg tcgtcgccga
     1321 cggccaccgc gccctggtgt tctcgcagtg gaccagcctg ctcgacctga tcgagcccca
     1381 cctccaggcc gccggcatcg cgttcacccg gctcgacggc accaccaccg accgcgccgg
     1441 cgtggtcgcg tgcttccagg ccgacggcgg cccgccggtc atgctgctgt cgctcaaggc
     1501 cggcggcacc ggcctcaacc tcaccgccgc cgaccacgtg ttcctgatcg acccgtggtg
     1561 gaaccccgcc gtcgaggatc aggccgccga ccgcgcccac cggatcggcc aggacaagcc
     1621 ggtgatggtc taccgcatgg tcgcgcgcga caccgtcgag gagcgcatcc tcgagctgca
     1681 ggaccgcaag cgcgcgctcg ccgacgccgc gctcgccgag gccggctccg ccgccgcgat
     1741 cacccgcgac gacctgctcg cgctgctcgc ctagcacgca gcaggcctgc taggtcagcg
     1801 gggcgagccg gaggatctgg ccgtggacgt cgcccgagtc cggatcgatc gccgggtcga
     1861 tgaacagccg ggtgaccgcg gtgttgcgca ggccggcgag catctcgtcg gagatcagcg
     1921 cgccggcgtc gagcccgatc gcgaacagct gctcgccggt gcccatcgcc cgggcgaacg
     1981 cctcggccgc ctggccggtc gagaacagga agccgtacgg ccggcggctc cacatgtcct
     2041 ggccgttcga cgtcaggtac cagatccgcg gcggcaggag ctcgaccagc cgctcgggcg
     2101 agacctcgtc cgcttggggc atggcccgtt gtacccggtc cggccgccgg gtgccgcgag
     2161 ggccgccgaa aactggtccc ggcacccggc cgggccgagg atcccggcgt gccgccgccg
     2221 ctgc
//
LOCUS       DASVOR010001619         2110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_162471,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001619 DASVOR010000000
VERSION     DASVOR010001619.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162471"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1810
                     /locus_tag="VF516_30330"
     CDS             <1..1810
                     /locus_tag="VF516_30330"
                     /inference="COORDINATES: protein motif:HMM:NF025394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEX8112083.1"
                     /translation="AADEPIPPPDAFQAMITLARTADGELRVGVGSMGGPPGATLLGR
                     FCHSDPALHAMVTANLAAEHALRPGVVFAEVVHLADGRLANISARPVLRDHEIVYLGR
                     SGAPAERQLPLTDLLVSVREGRVVLRSRSLGREVVPRLTNAHNFVNGALGTYRFLASL
                     QSQDSRALVLALGPLAQLPYVPRIRIGRVVLREASWTLRKAELEPVTGQAGAARIQAL
                     RRLRAATRLPRWVCVEDGDNVLPVDLDNALSVDSFAHLVKQRTGVTLTELWPAPDELV
                     VHGPDGRYASEIVVPFVRVPRTAAAQDRAAPATPPAAVRRSFPPGSEWLYAKLFTGQA
                     TADQILTETIAPIVADLPAWFFLRYGDPHWHVRFRVHGEPAQLFGEVMPRLSAAAQAM
                     LGDGRMWKLQLDTYEREVERYGGPVGIELAERLFCADSACVIDILGMLDGDAGNDARW
                     RLALRGIDQLLDDLGFDLAAKLAIMRGVREGFAREHRLETSIALQRGLGDKYRKERIP
                     LATLLDRAHDADSDLAPGLERIAGRSAANAPIVAELRAAEQRGRLTAPILDLVPSYIH
                     MHVNRLIRSAQRAHELVLYDLLVRLYESQAARAKPR"
     gene            complement(1818..>2110)
                     /locus_tag="VF516_30335"
     CDS             complement(1818..>2110)
                     /locus_tag="VF516_30335"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8112084.1"
                     /translation="ARLAVRATRAVQRTRAAIASVALGDQAIDLAEQNVAAEQKRFEL
                     GRSTNFDVLRRQDELEQARLRKASAVADYLAARTELDGLSGAILARYHIVMP"
BASE COUNT          256 a          811 c          760 g          283 t
ORIGIN      
        1 ggccgccgac gagccgatcc cgccgcccga cgcgttccag gcgatgatca cgctggcgcg
       61 gacggccgac ggcgagctcc gggtcggggt cggctcgatg ggcggtccgc ccggcgcgac
      121 gctgctcggc cggttctgcc acagcgaccc ggcgctgcac gcgatggtca ccgccaacct
      181 cgccgccgag cacgcgctgc ggcccggcgt cgtgttcgcc gaggtcgtcc acctcgccga
      241 cggccggctc gccaacatct cggcccggcc ggtgctgcgc gatcacgaga tcgtctacct
      301 cggccgctcg ggtgcgccgg ccgagcgcca gctcccgctg accgatctgc tggtctcggt
      361 gcgcgagggc cgcgtcgtgc tgcgctcgcg gtcgctcggc cgcgaggtcg tgccgcggct
      421 gaccaacgcc cacaacttcg tcaacggcgc gctcggcacg taccggttcc tggcgtcgct
      481 ccagagccag gacagccggg cgctggtgct cgcgctcggc ccgctggccc agctgccgta
      541 cgtgccgcgg atccggatcg gccgcgtcgt gctgcgcgag gcgtcgtgga ccctgcgcaa
      601 ggccgagctc gagccggtga ccggccaggc cggcgcggcg cggatccagg cgctgcgccg
      661 gctgcgcgcc gccacccggc tgccgcgctg ggtgtgcgtc gaggacggcg acaacgtgct
      721 gccggtcgat ctcgacaacg cgctgtcggt cgacagcttc gcgcacctgg tcaagcagcg
      781 caccggcgtg acgctgaccg agctgtggcc ggcgcccgac gagctggtcg tccacggccc
      841 ggacggccgg tacgccagcg agatcgtcgt gccgttcgtg cgggtgccca ggaccgcggc
      901 ggcccaggat cgcgccgcgc cggccacccc gccggccgcc gtccgtcgca gcttcccgcc
      961 cggctcggag tggctctacg ccaagctctt caccggccag gcgacggccg accagatcct
     1021 gaccgagacc atcgcgccga tcgtcgccga cctgccggcg tggttcttcc tccgctacgg
     1081 cgatccgcac tggcacgtcc ggttccgggt gcacggcgag ccggcgcagc tgttcggcga
     1141 ggtgatgccc cggctgtccg cggccgccca ggcgatgctg ggcgacggcc ggatgtggaa
     1201 gctccagctc gacacctacg agcgcgaggt cgagcgctac ggcgggccgg tcggcatcga
     1261 gctcgccgag cggctgttct gcgccgacag cgcctgcgtg atcgacatcc tcggcatgct
     1321 cgacggcgac gccggcaacg acgcgcgctg gcggctcgcg ctgcgcggca tcgatcagct
     1381 gctcgacgac ctcggcttcg acctggcggc aaagctggcg atcatgcgcg gcgtgcgcga
     1441 ggggttcgcc cgcgagcacc ggctcgagac ctcgatcgcc ctgcagcgcg gcctgggcga
     1501 caagtaccgc aaggagcgca tcccgctcgc caccctgctc gaccgcgcgc acgacgccga
     1561 cagcgacctc gcccccgggc tggagcggat cgccggccgc tcggcggcca acgcgccgat
     1621 cgtcgccgag ctgcgcgccg ccgagcagcg cggccggctg accgcgccga tcctcgacct
     1681 ggtcccgagc tacatccaca tgcacgtcaa ccggctgatc cgctcggcgc agcgcgccca
     1741 cgagctcgtg ctctacgacc tgctggtccg gctctacgag tcgcaggccg cccgcgccaa
     1801 gccgcgctga tccgacgtca cggcatgacg atgtgatagc gcgcgaggat cgcgccgctc
     1861 aggccgtcga gctcggtgcg cgccgcgagg tagtcggcga ccgcggacgc cttgcgcagc
     1921 cgggcctgct cgagctcgtc ctggcggcgc agcacgtcga agttggtcga cctgccgagc
     1981 tcgaaccgct tctgctcggc ggcgacgttc tgctcggcga ggtcgatcgc ctgatcgccg
     2041 agcgcgacgc tggcgatcgc ggcgcgggtg cgctggaccg cacgggtggc gcgcaccgcg
     2101 agccgggcct
//
LOCUS       DASVOR010001620         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395837,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001620 DASVOR010000000
VERSION     DASVOR010001620.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395837"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1534
                     /locus_tag="VF516_30340"
     CDS             <1..1534
                     /locus_tag="VF516_30340"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8112085.1"
                     /translation="HRAVTLGAAAGVLIGSCALVTRVKTEFVPDDDRSSFSVSVELPA
                     STALGATAEVVETLAADLRARCPGVVSTFVTVGRGTAQVNLGQIRVTLVPVRARPFSQ
                     QAVMGWIREHYAPLAQGGVKLTLGAAGGVGGDDSRPVQLNLRGRNMDALIAASDALVA
                     ELRKTRGFVDVDSSYRGGKPQIEVAPDRAAVSELGVPVSSIARTVRALVSRDKVTDYK
                     EDADLYDVRLTLADPVQRDFPALANLMVRSSTGELVPLSSLVHLERGVGPTQIAREAR
                     MRQITVYAGLDGLALGEATASAEAIARRVVPASIETEMSGTSQLMTESFGYMILSLIL
                     AVVLVYMILAAQFESFIHPFTIMMSLPLAVVGAFGALYLSGSNFSIFGMIGLIMLMGL
                     VTKNAILLVDFAQKQKAAGAATRDALLVAGPIRLRPILMTTAAMILGMLPVALALGEG
                     GEGRAPMAVVVIGGLVTSTLLTLVVVPVVFSLIEGLRDRLRRRRPPVAMVIDAGGGSG
                     GH"
     gene            complement(1500..>2107)
                     /locus_tag="VF516_30345"
     CDS             complement(1500..>2107)
                     /locus_tag="VF516_30345"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8112086.1"
                     /translation="TIALFGAASVASIGFAIVAAEIRAGALDRLDRAVELAIHRLDAR
                     PPDVVMEAAALIGANAVLLPAVAVVVALAIRQRRRAVAIILVIDTIVVIAAYSVLKVM
                     FSRERPRLFDKIALPTGYSFPSGHAMSAVGIYGVIAAALIALYPRARRPVIAAAIALI
                     ALIGLSRIYLGVHWPSDVIGGFLGGVPPLVVSVHLIHHPRR"
BASE COUNT          281 a          765 c          761 g          300 t
ORIGIN      
        1 ccaccgcgcg gtgaccctcg gcgccgcggc cggcgtgctg atcggctcgt gcgcgctggt
       61 cacccgggtc aagaccgagt tcgttcccga cgacgatcgc tcgtcgttct cggtgtcggt
      121 cgagctgccg gcgtcgaccg cgctcggtgc gaccgccgag gtcgtcgaga ccctcgccgc
      181 cgacctgcgc gcgcgctgcc ccggcgtggt gtcgacgttc gtcaccgtcg gccgcggcac
      241 cgcgcaggtc aacctcggcc agatccgggt cacgctggtc ccggtgcgcg cccggccgtt
      301 cagccagcag gccgtgatgg gctggatccg cgagcactac gcgccgctcg cccagggcgg
      361 cgtcaagctc acgctcggcg cagccggcgg ggtcggcggc gacgacagcc gcccggtcca
      421 gctcaacctg cgcggccgca acatggacgc gctgatcgcc gcgtccgacg cgctggtcgc
      481 cgagctccgc aagacgcgag ggttcgtcga cgtcgactcg agctaccgcg gcggcaagcc
      541 gcagatcgag gtcgcgcccg accgcgcggc ggtcagcgag ctcggcgtgc cggtgtcgtc
      601 gatcgcccgc acggtgcgcg cgctggtgtc gcgcgacaag gtcaccgact acaaggagga
      661 cgccgacctg tacgacgtcc ggctgaccct ggccgacccg gtgcagcgcg acttcccggc
      721 gctggccaac ctgatggtgc ggtccagcac cggcgagctg gtgccgctgt cgtcgctggt
      781 ccacctcgag cgcggcgtcg gcccgaccca gatcgcacgc gaggcccgga tgcggcagat
      841 caccgtgtac gccggcctcg acgggctcgc gctcggcgag gccaccgcat cggccgaggc
      901 gatcgcccgg cgcgtcgtgc cggcgtcgat cgagaccgag atgtcgggca ccagccagct
      961 gatgaccgag tcgttcggct acatgatcct ctcgctgatc ctggcggtcg tgctggtcta
     1021 catgatcctc gccgcgcagt tcgagagctt catccacccg ttcacgatca tgatgtcgct
     1081 gccgctcgcg gtggtcggcg cgttcggcgc gctctacctg tcgggctcga acttcagcat
     1141 cttcgggatg atcggcctga tcatgctgat gggcctggtc accaagaacg cgatcctgct
     1201 ggtcgacttc gcgcagaagc agaaggccgc cggcgccgcg acccgcgacg cgctgctggt
     1261 cgccgggccg atccggctgc ggccgatcct gatgaccacc gcggcgatga tcctcggcat
     1321 gctgccggtc gcgctcgcgc tcggcgaggg cggcgagggc cgcgccccga tggccgtcgt
     1381 cgtgatcggc gggctggtga cctcgacgct gctcaccctg gtcgtggtcc cggtggtgtt
     1441 ctcgctgatc gaggggctgc gcgaccggct gcgccgccgc cggccgccgg tcgcgatggt
     1501 catcgacgcg ggtggtggat caggtggaca ctgaccacga gcggcggcac gccgccgagg
     1561 aagccgccga tcacgtcgga cggccagtgc acgccgaggt agatccgcga tagcccgatc
     1621 agcgcgatca gggcgatcgc ggcggcgatc accggccggc gcgcccgcgg gtagagcgcg
     1681 atcagcgcgg cggcgatcac gccgtagatc ccgaccgccg acatcgcgtg gccgctcggg
     1741 aagctgtacc cggtgggcag cgcgatcttg tcgaacagcc gcggccgctc gcgcgagaac
     1801 atcaccttga gcacgctgta cgcggcgatc acgacgatcg tgtcgatcac caggatgatc
     1861 gcgacggcgc gcctgcgctg ccggatcgcg agcgcgacga ccacggcgac cgccggcagc
     1921 agcaccgcgt tcgcgccgat cagggccgct gcctccatca ccacgtcggg cggccgcgcg
     1981 tcgagccggt ggatcgcgag ctcgaccgcg cggtcgagcc ggtcgagcgc gccggcgcgg
     2041 atctcggcgg cgacgatcgc gaacccgatg ctggcgacgc tcgcggcgcc gaacagcgcg
     2101 atggtgc
//
LOCUS       DASVOR010001621         1825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_105126,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001621 DASVOR010000000
VERSION     DASVOR010001621.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105126"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VF516_30350"
     CDS             complement(<1..468)
                     /locus_tag="VF516_30350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112087.1"
                     /translation="MLPRRVQPLAGWSIVVVFALIGAALLATVWSTRASILDASEAVR
                     CGQADAVEQAVRAELSDLDGVPTSADLATILHELHDDGLRYLAMIEREGGAPRGIDGR
                     GRLDVVAAAGAPVGSGSANLFARSRAVGCKADESGGRLRMELRTLRRLKSGGRT"
     gene            complement(488..961)
                     /locus_tag="VF516_30355"
     CDS             complement(488..961)
                     /locus_tag="VF516_30355"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEX8112088.1"
                     /translation="MLTKLKLAMLISAPLIAGAATYAAAQGSAPSRETLIQKFDKNGD
                     GVLDDAERADMKAAFAAKRAAHRQAMLDKYDTDKDGKLEPAERAAMRNDLLTRRFQKM
                     DKNGDGVLSLDEFKAGAAMPGHRHGRFGHRHHRAVQGAQGGGISGGGGQGQGTKL"
     gene            1129..>1825
                     /locus_tag="VF516_30360"
     CDS             1129..>1825
                     /locus_tag="VF516_30360"
                     /inference="COORDINATES: protein motif:HMM:NF019359.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8112089.1"
                     /translation="MAFTVGELSRLTGLTVRALHHYDEIGLVRPSQRTAAGYRLYDET
                     DALRLQQVLVLRELGVPLDQIGAAIDAAADRAALLREHRAALVEKRGRLDRMLAAVDA
                     ALAVLEKGKTQMQSEDFKAMFDGFDPAEHEAEAKERWGHTEAYRESARRTQKYGKPEW
                     EAIRNESEEIYARLGKLMQQGAAVTDPAVQAAVEDHRRHIERWFYPCSPQMHKGLGEM
                     YVADPRFTATLDGK"
BASE COUNT          257 a          631 c          686 g          251 t
ORIGIN      
        1 ggtccggccg ccggacttga gccggcgcag cgtgcgcagc tccatgcgca gccggccgcc
       61 ggactcgtcg gccttgcagc cgaccgcgcg gctgcgcgcg aacaggttgg cgctgccgct
      121 gccgaccggg gcgccggcgg cggcgacgac gtcgagccgg ccgcgaccgt ctatcccacg
      181 gggggcccca ccctcccgct cgatcatcgc gaggtagcgc aggccgtcgt cgtggagctc
      241 gtgcaggatc gtggcgaggt cggccgacgt cggcacgccg tcgaggtcgg acagctcggc
      301 gcgcaccgcc tgctcgaccg cgtcggcctg gccgcagcgc acggcctcgg acgcgtcgag
      361 gatcgacgcg cgcgtgctcc acaccgtggc gagcagcgcg gcgccgatca gcgcgaacac
      421 cacgacgatc gaccacccgg cgagcggctg gacacggcgg ggcagcatcg ccgggagcat
      481 acccggctca gagcttggtg ccctggccct ggccgccgcc ccctgagatg ccgccgccct
      541 gcgcgccctg gaccgcgcgg tggtggcggt ggccgaaccg gccgtggcgg tggcccggca
      601 tcgccgcgcc ggccttgaac tcgtcgagcg agagcacgcc gtcgccgttc ttgtccatct
      661 tctggaaccg ccgggtcagc aggtcgttgc gcatcgccgc gcgctcggcg ggctcgagct
      721 tgccgtcctt gtcggtgtcg tacttgtcga gcatcgcctg gcggtgggcg gcgcgcttgg
      781 cggcgaatgc ggccttcatg tcggcgcgct cggcgtcgtc gagcacgccg tcgccgttct
      841 tgtcgaactt ctggatcagg gtctcgcgcg acggcgcgct gccctgggcg gcggcgtagg
      901 tggcagcgcc ggcgatcagc ggcgcagaga tcagcattgc gagcttgagc ttggtcaaca
      961 tcggtcgttc ctccggtgtg gcgctatcgc aacgcgcgtg ccggcggtaa gtgcgcgaac
     1021 cgcggtaccc tggggcagtt cgggctgcca aagtcgcagc gcgcggctgc agatcccgct
     1081 tgaccctcac gcgacgtgag gccgcacggt gcagctcacg aggaccccat ggcattcacg
     1141 gttggagagc tgtcccggtt gaccgggctg acggtgagag cgctgcacca ctacgacgag
     1201 atcggactgg tccggccgtc gcagcggacc gcggcgggct accggctgta cgacgagacc
     1261 gacgcgctgc ggctccagca ggtgctggtg ctgcgcgagc tcggcgtccc gctcgaccag
     1321 atcggcgcgg cgatcgacgc ggcggcggac cgcgcggcgc tgctccgcga gcatcgcgcg
     1381 gcgctggtcg agaagcgcgg ccggctcgac cggatgctcg ccgccgtgga tgccgcgctg
     1441 gcggtgctcg agaaaggcaa gacacagatg cagtccgagg atttcaaggc gatgttcgat
     1501 gggttcgacc cggcggagca cgaggccgag gccaaagagc gctggggcca caccgaggcg
     1561 taccgggagt ccgcgcgccg gacccagaag tatggcaagc cggagtggga ggcgatccgc
     1621 aacgagagcg aggagatcta cgcccggctc ggcaagctca tgcagcaagg cgcggcagtg
     1681 accgatcccg cggtgcaggc cgcggtcgag gaccaccgcc ggcacatcga gcgctggttc
     1741 tatccgtgct cgccgcagat gcacaagggg ctcggcgaga tgtacgtcgc ggacccgcgg
     1801 ttcacggcga ccctcgacgg caagg
//
LOCUS       DASVOR010001622         7864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_133371,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001622 DASVOR010000000
VERSION     DASVOR010001622.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133371"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1573)
                     /locus_tag="VF516_30365"
     CDS             complement(<1..1573)
                     /locus_tag="VF516_30365"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8112090.1"
                     /translation="MPRTRTHDAPRDPDPPIAIVGMAGRFPGAADIESFWRLLMARGD
                     AIRPAPAERWNTVAELDPEAPVQRVGGFLDGVDEFDPAFFGISPREAEDIDPQHRLML
                     EVIWQALEDAAQPAAALRGSHTGVYVGASWHDYEILRKERGANATQHSGPGNALDMIA
                     ARVSYFLQLTGPSLTVETGCSSSLVALHLACQALRCGEIDGAFVGGVNLVLAPDVSVA
                     LTRFGGLSPEGRCKAFAASADGFVRGEGAIAIYVKTLARARADGDRIHAVIAGTAVNN
                     DGGGDSLVTPSPSGQEALLTRAYAQARIPADAVVYVEAHGTGTPVGDPIEAEALGRAL
                     GLRRTGAAGPLAIGSVKTNIGHLEAAAGLAGLVKTVLALAHRVVPANLHGDALNPAIA
                     FDALHLHVVRAPLVLPPHGPLYAGVNSFGWGGTNAHVILRSAPAEASSASSAPPAWAP
                     PAWAPIVVPLSAHSEDALRQRAMDVQAVIATGAAPVEAVAATLAWKRDQFPHRAAFVV
                     ETAAELEARLAGFAAN"
     gene            1910..2515
                     /locus_tag="VF516_30370"
     CDS             1910..2515
                     /locus_tag="VF516_30370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8112091.1"
                     /translation="MDVEVEIARLRAAGQLKEVATLAIESYGPEVLSFLEMMLRDHAD
                     SSDAFAQACEDLWQGLPRFEGRAAMKTWFYTLARHAASRLRRTTKPERFAKVSEITDV
                     ADRVRSRTRPYLRTEVKAGFAAIRASLDETDRLLLVLRVDRAMSWNDVARVMTDDGDD
                     DSDKGIARAAARLRKRFQTVKESIRERAVAAGLIARPDDEV"
     gene            complement(2518..6216)
                     /locus_tag="VF516_30375"
     CDS             complement(2518..6216)
                     /locus_tag="VF516_30375"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112092.1"
                     /translation="MGPGTGVAAGTSKVRIDAEVGPQGKFILRQEIGSGAMGRVYRAF
                     DRYLLRDVAIKFILRPEGMNHDDFMALFWQEAHIIARLDQHDNIVRILDVERSAYPPF
                     IVMEYLDGQSLEKLLRRAPLDAPMILQIMIAVARGLGEAHMKGVCHRDLKPSNIFLQK
                     TGRVKLLDFGLAQIRNHLFETSTNKASFVATHAVPPLASAGTPGYMAPEQWRGEDADA
                     ATDLWAVGAILYRLFGKAPPFDADTLAALAARVLSGEPPVPLIRLCPDAPVEVCELVE
                     RLLRFERSRRPSQVSDIIPLLEAALHKILNDTSQPPVALAAMRRSPAGASEVRPAVSA
                     THRGAPWSAPDGAVRFWDRMLLVLVTGADSDPRIVPDHLPAWVLRKAGLDCEVLHCPA
                     VEMGDVGEGAIRLRAYLNAFTTRPRHMFFITHGEGTHVVLRMLLDEARRLRWPDDGKF
                     ELDMRSPFYRVRHVAILRPGRGVATAHHVAGLHASLDTAPLVAEVVSEAQHFLAANLP
                     SPAIHYFGFDDDGAASHAGSPFVKALATMLTRPEIMVARETIAQTFELDCATRIASLV
                     GPIEGDGSSDVSPTVSGANAATQAEVFDDLLTLARSQHRRPVAIVITGDAGVGKSTVL
                     RMAARRLCSELITDGSAVIPILIPLYFASLTAEQLSVLDSKASDDQRGRMLLDILLGW
                     WCEWFDGTSYMGAVRTDWILACMRSEPVMLIFDGVDEFITNHPTLTLSDFQRMLAFLG
                     AEHRKNGWLTSALGVRSTQPGLPLLASNNLREVLRLTTAQAMRQFPAASSWLSAGGNA
                     PLKKLLSTPLILAQLNSRRPSAMAPASTNGEVILLALTTIIEQSDLCGRLDENGQPID
                     AQRWIDALMAAAWCMFRRLRGEIATETLRLDGAELHRSWQAHLERTGQQAQGAHLLSG
                     LQLLCEVRAFDALLQRTILYPTGRGEVRFIHREWQDFLAARYLAQVVVYRHVDEFRHV
                     GNTARISQVAGELLGQTGMCIDEAFVLALLRHAQNTGARLITANFSALLTNSRVLMEG
                     PAIDAFLSAVNRAPPVARFITLSGLGYRALRGDDASAQDLRHRLVRVFRDQLSAPMVE
                     DELSLMRSLAWCYLKAYVQRFGGPAMSEPWPGLGDRAEPGALAMMCSVSDEGPRFLVE
                     HRSIQMALLELQQVVPEDASRPISGVHYLYCLAVARRYGGGIAELGRELPTMLAHGSP
                     YATAIERYQVVPELRDILAACRRLELPP"
     gene            complement(6510..6746)
                     /locus_tag="VF516_30380"
     CDS             complement(6510..6746)
                     /locus_tag="VF516_30380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112093.1"
                     /translation="MFEGARSIEIDVHKDTPVLPLPSPPEPHHWTVYHTDKQSNSLCA
                     GASCIWVDRRGKFCSVGRRRKSGFLGCSRDGSAW"
     gene            complement(6822..7436)
                     /locus_tag="VF516_30385"
     CDS             complement(6822..7436)
                     /locus_tag="VF516_30385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112094.1"
                     /translation="MAHVSPHGMPSGRSRGRGGDRLGSVLWMIVILGGGVVGMIFVVG
                     CCQGDRDRRASPPPASSPSSPALGSAAPSPSLPAPLAPTTTVTAVLAQGRTFPDSLVT
                     VRGRVSHPFMTWNCPPGAICKTRPPEAPVMIIADVDASAKDELWVIKCPEQSTGFAPD
                     VGMLVVVRGVVRADPRGGQIKLEHRETIFVAADGSALGPPWTTR"
     gene            complement(7521..>7864)
                     /locus_tag="VF516_30390"
     CDS             complement(7521..>7864)
                     /locus_tag="VF516_30390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112095.1"
                     /translation="GFNPYSLWVANWNVRCPNIPNPPWTRWTFWQSSATGTVAGFSDP
                     VDVDNFNGTSADLMVFVKSPSGPSELVSPLLGSALDGDPGAEPSDGGNGRAAAGEPEA
                     DLGMSGSPSLP"
BASE COUNT         1303 a         2680 c         2739 g         1142 t
ORIGIN      
        1 ggttcgccgc gaagcctgcc aggcgcgcct cgagctccgc cgcggtctcg accacgaacg
       61 ccgcgcggtg cgggaactga tcgcgcttcc acgcgagcgt tgccgcgacc gcctcgaccg
      121 gggcagcgcc ggtcgcgatc acggcctgta cgtccatggc gcgctggcga agcgcgtcct
      181 cgctgtgcgc cgacagcggc accacgatcg gagcccaggc cggcggcgcc caggccggcg
      241 gcgcgctcga cgcgctcgat gcctcggccg gtgcgctgcg caggatcacg tgcgcgttcg
      301 tgccccccca gccgaacgag ttgacgcccg cgtacagcgg cccgtgcggg ggcagcacca
      361 gcggcgcgcg gacgacgtgc aagtggagcg catcgaacgc gatcgccggg ttcagcgcgt
      421 cgccatgcag gttggccggg acgacgcgat gcgcgagcgc gagcaccgtc ttcaccagcc
      481 cggcgaggcc ggccgcagcc tcgaggtggc cgatgttggt cttgaccgag ccgatcgcca
      541 gcggacccgc cgcgccggtc cggcgcaggc cgagcgcgcg accgagggct tcggcctcga
      601 tcggatcgcc gacgggggtt ccggtgccat gcgcctcgac gtagacgacc gcgtcggccg
      661 ggatccgcgc ctgcgcatag gcgcgggtca acagcgcctc ctggcccgac gggctcgggg
      721 tgaccaggct gtcgccgccg ccgtcgttgt tcaccgcggt gccggcgatc acggcgtgga
      781 tccgatcgcc gtcggcgcgc gcgcgcgcca gcgtcttcac gtagatcgcg atcgcgccct
      841 cgccgcggac gaagccatcg gccgaggccg cgaacgcctt gcaccgcccc tcgggtgaca
      901 ggccaccgaa ccgggtgagc gcgaccgaga cgtcgggggc gagcaccagg ttgaccccgc
      961 cgacgaacgc gccgtcgatc tcgccacagc gcagcgcctg gcacgccagg tggagtgcga
     1021 ccagcgacga cgagcacccg gtctcgaccg tgaggctcgg cccggtcagc tggaggaagt
     1081 acgacacgcg cgccgcgatc atgtcgagcg cgttgccagg gccgctatgc tgcgtggcgt
     1141 tcgcgccgcg ctccttgcgc aggatctcgt agtcgtgcca cgacgcgccg acgtagacgc
     1201 cggtgtgcga accgcgtagc gccgcggcgg gctgcgcggc gtcctcgagc gcctgccaga
     1261 tcacctcgag catgaggcgg tgctgggggt cgatgtcttc cgcctcgcgc ggcgagatcc
     1321 cgaagaacgc cggatcgaac tcgtcgaccc cgtcgaggaa gccgccgaca cgctgcaccg
     1381 gggcctcggg atcgagctct gcgaccgtgt tccagcgctc ggcgggagcc gggcgaatcg
     1441 cgtcgccgcg cgccatcagc aggcgccaga agctctcgat gtcggccgcg cctggaaacc
     1501 ggccggccat gcccacgatg gcgatgggcg ggtccggatc gcgaggcgcg tcgtgcgtgc
     1561 gggtgcgagg catggcgggg cgagcgcggc gcgaatccga aagacgcacg ccacccacat
     1621 cgacgcgacg accgctgggc gcggtcacgt ctcggagcga gacgtggacg tcatcactgc
     1681 agcaatgtgc tgcaccccgt ttgtcctcga gtcgccaagg ctcaacggaa tagagacgcc
     1741 acgtccacga tgtgagccat tttgccgcca gatcccctgg acggacgcgc gtcaaccacg
     1801 ctgcctgacg agcgccccgc agccgatcga gcgctgcggg ggggccttgc ggtatcctgc
     1861 ggcgcccaac cgtggcgcgg cagcgccgga aggcatgcaa ggtctaccca tggatgtaga
     1921 ggtggagatc gcgcggctac gtgcagcggg ccagctgaag gaggttgcga ccctggcgat
     1981 cgagagctac ggccccgagg tcctcagctt cctcgagatg atgctccgcg accatgcgga
     2041 ctcgtcggac gcgttcgccc aggcttgcga ggacctgtgg cagggcctgc cgcgcttcga
     2101 gggccgcgct gcgatgaaga cgtggttcta caccctggcc cggcacgcgg cctcgcggtt
     2161 gcggcgcacc accaagcccg agcggttcgc caaggtcagc gagatcaccg acgtggcgga
     2221 tcgcgtgcgc agccgcaccc ggccgtacct gaggaccgag gtcaaggccg ggtttgccgc
     2281 gatccgcgca tcgctcgacg agaccgaccg cctgttgctc gtgctgcgcg tcgatcgcgc
     2341 gatgagctgg aatgacgtcg cccgggtgat gaccgacgac ggcgacgacg attcggacaa
     2401 gggcatcgcg cgtgccgcgg cgcggctccg caagcggttc cagaccgtca aggagtcgat
     2461 ccgcgagcgc gcggtcgcgg cgggcttgat cgcgcgcccc gacgacgagg tctgacgcta
     2521 cggggggagc tccagccggc ggcacgccgc gaggatgtcg cgcagctcgg ggacgacctg
     2581 gtagcgctcg atcgccgtcg catacggaga gccgtgtgcc agcatggtgg gcagctcgcg
     2641 tccgagctcg gcgatgccac caccgtagcg ccgcgccacg gccaggcagt agaggtaatg
     2701 caccccggag atcgggcgac tggcatcctc gggcacgacc tgctggagct cgagcaacgc
     2761 catctggatc gaacgatgtt cgacgaggaa gcgcggccct tcgtcgctga ccgagcacat
     2821 catggccagt gcgccgggct cggcgcggtc accgagcccg ggccagggct cggacatcgc
     2881 gggaccgccg aaccgctgca cgtaggcctt gaggtagcac caggcgaggc tgcgcatcaa
     2941 gctgagctcg tcttccacca tcggggcgga cagctggtca cgaaacaccc gcacgagccg
     3001 gtgccgcaga tcctgcgccg aggcgtcatc accgcgcagc gcccggtagc caaggccgct
     3061 cagggtgatg aagcgcgcga ccggcggcgc gcggttgacc gccgacagaa aggcgtcgat
     3121 cgcaggacct tccatcagca cgcgtgaatt ggtgagcagc gcggagaagt tcgcggtgat
     3181 cagccgcgcg ccggtgttct gcgcgtggcg cagcagcgcg agcacgaatg cttcatcgat
     3241 acacatcccc gtctggccga gtagctcgcc ggcgacctga gagatccgcg ccgtgttacc
     3301 aacatggcgg aactcatcga cgtgacgata tacgaccacc tgcgcgaggt agcgggccgc
     3361 caggaagtcc tgccactcgc gatggatgaa ccggacctcg ccacggccgg tcgggtacag
     3421 gatcgttcgt tgcagcagcg cgtcgaatgc acggacctcg cacagcagct gaaggccgga
     3481 caggaggtgc gcaccctggg cctgctgccc cgtgcgctcg aggtgggctt gccacgagcg
     3541 gtggagctcg gcaccatcga gccgtagcgt ctccgtcgcg atctcgcctc gcaggcgccg
     3601 gaacatgcac cacgctgcgg ccatcagcgc gtcgatccag cgctgcgcgt cgatcggctg
     3661 cccgttctca tccagccgcc cgcacaggtc gctctgttcg atgatcgtcg tcagcgcgag
     3721 caggatgacc tcaccgttcg tgctcgccgg ggccatcgca gacggccggc gggaattcag
     3781 ctgcgcgagg atcagcggcg tggacagcag cttcttgagc ggcgcattgc cgccggcgct
     3841 cagccagctc gaggccgcgg ggaattgccg catcgcctgc gccgtggtga gccggagcac
     3901 ctcgcgaagg ttgttggagg ccagaagcgg caggccgggc tgagtgctgc ggacgccgag
     3961 cgcgctggtg agccagccgt tcttgcggtg ctcggccccg aggaacgcga gcatccgctg
     4021 gaagtccgag agggtgagcg tcgggtggtt cgtgatgaac tcgtcgacac catcgaagat
     4081 cagcatcacc ggctcggacc gcatacacgc caggatccag tccgtcctga ccgctcccat
     4141 gtagctggtg ccatcgaacc actcacacca ccagcccagc aggatgtcga gcagcatccg
     4201 accgcgctga tcgtccgaag ctttgctgtc gagcaccgac agctgctcgg cggtcaggct
     4261 cgcgaagtac agcgggatca agatcgggat caccgcgctg ccatccgtga tcagctcgct
     4321 gcacaggcga cgcgcggcca tgcgcagcac cgtggacttc ccgacccccg cgtcgccggt
     4381 gatcacgatc gccaccgggc ggcggtgctg ggagcgcgcg agggtgagca ggtcgtcgaa
     4441 cacctcggcc tgggtcgccg cgttggcgcc ggacaccgtc ggggacacgt cgctgctgcc
     4501 gtcgccctcg atcgggccca ccagcgaggc gatccgggtg gcgcaatcga gctcgaaggt
     4561 ctgcgcgatg gtctcgcgcg cgaccatgat ctcggggcgg gtgagcatcg tggcgagcgc
     4621 cttcacgaac ggcgagccgg cgtgtgacgc cgccccgtcg tcgtcgaacc cgaagtaatg
     4681 gatcgccggg ctcggcaggt tggcggcgag gaagtgctgg gcctccgaga ccacctccgc
     4741 gaccagcggt gcggtgtcca ggctcgcgtg cagaccggcg acgtggtgtg cggtcgcgac
     4801 gccgcgtccg ggccggagga tcgcgacatg acggacccgg tagaacggcg agcgcatgtc
     4861 gagctcgaac ttgccatcgt cgggccaccg cagccggcgc gcttcatcga gcagcatccg
     4921 gagcacgacg tgggtgcctt cgccatgcgt gatgaagaac atgtgacgtg gtcgcgtggt
     4981 gaacgcgttg aggtacgccc ggagacggat cgctccctca ccgacatcgc ccatctccac
     5041 cgccgggcag tgcaggacct cgcagtcgag gccggccttg cgcaggaccc aggctggcag
     5101 gtgatccggc acgatgcgag ggtcagagtc cgccccggtc accagcacga gcaacatcct
     5161 gtcccagaag cgcactgcgc cgtccggtgc gctccacggg gcgccccggt gagttgcgct
     5221 gactgcaggc cggacctcgg aggccccggc cggcgagcga cgcatggccg cgagcgccac
     5281 cgggggttgc gacgtatcat tcaggatctt gtgcagcgcg gcttcgagga gcgggatgat
     5341 atcggatacc tggctcggcc gccgagaccg ctcgaagcgc aggaggcgtt cgaccagctc
     5401 gcagacctcg accggcgcgt cggggcacag cctgatcagc gggaccggcg gctcaccgga
     5461 cagcacgcgc gcggcgagcg cagccagcgt atcggcgtcg aacgggggcg ccttcccgaa
     5521 cagccggtac aggatcgccc ccaccgccca cagatcggtg gccgcgtcgg cgtcctcgcc
     5581 ccgccattgc tcgggcgcca tgtacccggg cgtccccgcg ctggccagcg gcgggaccgc
     5641 gtgcgtcgcg acgaagctgg ccttgttcgt cgaggtctcg aacaggtgat tccggatctg
     5701 ggccagaccg aagtcgagca gcttcacgcg gcccgtcttc tggaggaaga tattgctcgg
     5761 cttgaggtcg cggtggcaga cccccttcat gtgcgcctcg ccgagcccgc gcgccaccgc
     5821 gatcatgatc tggaggatca tcggcgcatc gaggggggca cgccggagca gcttctcgag
     5881 ggactgtcca tcgagatact ccatcacgat gaacggtgga taggccgatc gctcgacgtc
     5941 gaggatccgc acgatgttgt cgtgctgatc gaggcgcgcg atgatgtgtg cctcctgcca
     6001 gaacaacgcc atgaagtcat catggttcat gccctcgggg cggaggatga atttgatggc
     6061 gacatcgcgc aacaggtatc ggtcgaacgc gcgatacact cgccccatcg caccactacc
     6121 gatctcctgt cgaaggatga acttgccctg tggcccgacc tcggcgtcga tgcgaacctt
     6181 cgaggttccg gccgccacgc cggtcccggg ccccacggtc ctggcagcga cgcctcgaag
     6241 cagagaatcg tagaggctca ggacctcgag ctcccccgag gaggggccga gcgtgagagc
     6301 ggtagggtcg ttcccgactc cgccaccgct cccatggggg acctcaccat gctcgctcat
     6361 gtcgcagttc acccatttct aacacacaca cagcatgatc taggaaaatt accgtgtcct
     6421 cgctctcaat tctgtgggag ccacgactca ctgccacctc ggaaccacct aagcccctga
     6481 tacaaggtgg ttccatggca agtcatgtct caccacgcgc tgccgtcgcg gctacatccg
     6541 aggaatccgg attttcgccg tcgaccgacc gagcaaaatt tgccgcggcg atccacccag
     6601 atgcagctcg ctcctgcgca cagcgagttc gattgcttgt cggtgtgata gacggtccag
     6661 tggtgcggct ccggggggct gggcaagggg aggacgggcg tgtccttgtg cacgtcgatc
     6721 tcgatcgatc tcgcgccttc gaacacgagc tgatcatcgc atctacgtga gccggcacat
     6781 acggttacgg gtctccgcga gacggtacgc tcccggcgcc gtcagcgcgt ggtccacggc
     6841 gggccgagcg cagatccatc cgccgcgacg aagatcgtct cgcgatgctc gagcttgatc
     6901 tggccgccgc gcggatctgc ccggaccacg ccacggacga cgacgagcat gccgacgtcg
     6961 ggtgcaaagc cggtcgattg ctcggggcac ttgatgaccc acagctcgtc cttcgcgctc
     7021 gcatcgacgt ccgcgatgat catcaccggc gcctcgggcg gccgggtctt gcatatcgcg
     7081 ccgggcgggc agttccacgt catgaacgga tggctcacgc ggccgcggac cgtgaccagc
     7141 gaatcgggga acgtacggcc ctgcgcgagc actgcggtca cggtcgtggt cggagcgagc
     7201 ggggcaggca gcgacggcga cggcgccgcc gaccccagcg cgggcgacga cggcgagctc
     7261 gcaggcggag gcgaagcgcg tcgatcgcga tcgccttgac agcaacccac cacgaagatc
     7321 atgccgacca caccgccgcc gaggatcacg atcatccaca gcacgctgcc gaggcggtcg
     7381 ccgcccctac cgcgcgagcg acccgaaggc atgccgtggg gcgatacgtg ggccacgccg
     7441 agatggtagc tcagcaccta ggacgaacaa atggcctctg cagggtgctc gccgaggcgc
     7501 agaggcagga gacgaccctc tcagggtaga gagggtgagc ccgacatgcc caggtctgcc
     7561 tcgggttccc cggctgctgc gcggccattt ccaccgtcag aaggctcggc gccagggtcg
     7621 ccatcgagcg ccgatccgag caacggcgat acgagctcgc ttggccccga cggtgacttc
     7681 acgaacacca tgaggtccgc ggacgtcccg ttgaagttgt cgacatcgac cggatcgctg
     7741 aatccagcga cggtgccggt ggcagaggat tgccagaagg tccagcgcgt ccatggtggg
     7801 tttggaatgt tcgggcatcg cacgttccag ttcgcgaccc agagggaata cggattgaag
     7861 ccgg
//
LOCUS       DASVOR010001623         6478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_192237,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001623 DASVOR010000000
VERSION     DASVOR010001623.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6478
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192237"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VF516_30395"
     CDS             complement(<1..466)
                     /locus_tag="VF516_30395"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8112096.1"
                     /translation="MPEEDSMVAINSPTPQPSAKPAPAPAHGPRIVVERRSASQITDT
                     PAVFDCENVEVYYGAFRAVRGVTLTIHAHQITAFIGPSGCGKTTVLRSLNRMHDVIPG
                     ARVAGRVSYHGIDLYAPEVSATEVRRRIGMVFQKPNPFPKSIYDNIAFGPRVN"
     gene            complement(469..1386)
                     /gene="pstA"
                     /locus_tag="VF516_30400"
     CDS             complement(469..1386)
                     /gene="pstA"
                     /locus_tag="VF516_30400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEX8112097.1"
                     /translation="MTTASAFRESTVNRQRRNDLRARPSWRTVKNRLMLGLMIAALVL
                     VAIPLVAVLWSVIVRGGSTAFKDFPSFFTETIPRISRLPGPGMGPAIVGTLLATGGAT
                     LIAVPLGILGAVYLHEYGGAGKFARLVRFMATVMTGVPSIVMGLFIYLMWTLSLGYSA
                     FGGALALACLMLPVVIGSTEQMLRLVPPQLREAAYALGTTKSRTVLTVVLPAALPGIV
                     SGSLLAIARAAGETAPLLFTVGAATAFNPHLFSQANTALSLQIFSNAVSAFAPAQDRA
                     WGAALTLMMVTFIVTLVARFFTARFALKR"
     gene            complement(1383..2249)
                     /gene="pstC"
                     /locus_tag="VF516_30405"
     CDS             complement(1383..2249)
                     /gene="pstC"
                     /locus_tag="VF516_30405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEX8112098.1"
                     /translation="MQLSELRGHKQYADRAFRGIAIAAAGLVLLVLGMIAIRMTLRAW
                     PALGLEFFTSTRWSAPDGRFGALPFIWGTLYTAAIAVILAVPVSLGVALFITQVAPPW
                     LKTPLVYLLDLLAVVPSVVFGLWGVLVLSQHFGLSRSFLTAGVILAVMIIPIITSLAR
                     EVIETTPASEKEAALALGATRWEMITASVLPHSKGGITGAVMLGLGRAMGETIAAALV
                     IGSALGQITINPLAPGNSMPAVIANEWGEADEHHKAALIALAVVLFIITIIVNLIANS
                     IVQRSMKRSRGS"
     gene            complement(2249..3337)
                     /gene="pstS"
                     /locus_tag="VF516_30410"
     CDS             complement(2249..3337)
                     /gene="pstS"
                     /locus_tag="VF516_30410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEX8112099.1"
                     /translation="MYKMIAFSALAALCVGGCNKKKDESGGSTATMTKEDKGPAKPPE
                     TGPVTINGSGSTFQKQFQELAIEGFSKVNKDVKVNYGAGGSGKGRQDFSDMVTDYGCT
                     DGLFKEADAAKAKGGPFLYFPILLGAITVSYKLDGVDKLQLSAPTIAKIFQREIKKWD
                     DKDIAADNPGVKLPATDIVVAHRSDGSGTTEQFTLYLNTAAPDQWKLKSGSTVEWPAD
                     TQAGNGNGGVAQIVKSTPGAIGYVDLPDAKAAGLKFASVKNQAGKFIEPSSASASAAG
                     DGIDVKDNLTYVSVNAKGDAAYPITAPTWCVAYAKQTDKAKGAAVKAYFKHMLTDAQA
                     QVKDIDFAPLPKSLDDKAVAQLDKIQVP"
     gene            complement(3517..4197)
                     /gene="phoU"
                     /locus_tag="VF516_30415"
     CDS             complement(3517..4197)
                     /gene="phoU"
                     /locus_tag="VF516_30415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEX8112100.1"
                     /translation="MTAHTSKDFEQELRTLRERLATMGERAARQIALAMKALADKDDE
                     LARDVIRNDSQIDRDENDVDELALQILATRQPVASDLRFITMALKFVVDLERIGDLAG
                     GIAKRALELNRLPSLEPRIDLSKLATLVQKNLQAALDAFVRKDAESATAVITADAEID
                     KLNASLFAELIAHVATDPATVTRVLPLTSVCRYLERVGDHVKNLAEEVVYMVKATDVR
                     HRPILPQN"
     gene            complement(4194..5573)
                     /locus_tag="VF516_30420"
     CDS             complement(4194..5573)
                     /locus_tag="VF516_30420"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112101.1"
                     /translation="MIQRVRLRLLGLALLVSIVAGTLATAVLHPQLASDALSIAIAAF
                     AVSIVIGWVVTVILRGAIRRVVKSMRDVVRDPSRRIRPDGAVGDELRELTEWINFLAD
                     DADKSHRALVRERMLLAAVADGSTQGVIAVDSEHRIELLNDAARKMLGVTSSPVGEPL
                     IDFVRVPELFDLIDSDVAATAEVSLPNTPRALIRTARKWGGDGRVLLLEDVTTVRRLE
                     TVRRDFVANVSHELRTPVAVIRANAETLMAGAKDDPAIAGRLIDGLHRNAERLARILA
                     DLLDLSRLDAGQYRLELADVAVRPVIEQSLSAVEPQAAQRNVTVRVDIPTDLAVRADP
                     KALDHILVNLIDNGVKYGRPDGNVWVEARPVGDGAADGVRPEGVRIEVRDDGPGIADK
                     HRTRVFERFYRADPSRSREAGGTGLGLSIVKHLVESMDGEVGVEPNLPHGSIFWLRLP
                     RASNMGTPP"
     gene            complement(5570..6268)
                     /locus_tag="VF516_30425"
     CDS             complement(5570..6268)
                     /locus_tag="VF516_30425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8112102.1"
                     /translation="MLVLVIEDEVDLATTLEYNLRAEGFTVRLAHSGRQGLASATADP
                     LPDVIVLDLMLPDLSGTEICRKLREGERTRDIPVVMCTAKGEEIDRVVGFEVGADDYV
                     VKPYSVRELILRIRALLRRSHRVEGEPSLIRFGRLKIDRDAHRAWVDDAEIGLTALEF
                     RLLHAFLSRRGRVQTRDALLSDVWGIEADVTTRTVDTHVKRLREKLGDAGAYIETLRG
                     VGYRFKDQPDEATA"
BASE COUNT          954 a         2186 c         2302 g         1036 t
ORIGIN      
        1 cgttgacccg ggggccgaac gcgatgttgt cgtagatgct cttggggaac gggttcggct
       61 tctggaacac catgccgatc cggcgccgga cctcggtcgc cgagacctcg ggcgcgtaga
      121 ggtcgatgcc gtggtagctg acccgcccgg cgacccgtgc gccggggatc acgtcgtgca
      181 tccggttgag cgagcgcagc accgtggtct tgccgcagcc cgacgggccg atgaacgcgg
      241 tgatctggtg ggcgtggatc gtcagggtca cgccgcgcac cgcccggaac gcgccgtagt
      301 agacctcgac gttctcgcag tcgaacaccg ccggcgtgtc ggtgatctgg gaagccgacc
      361 tgcgctcgac gacgatgcgc gggccatgcg ccggggcggg cgcgggcttg gccgagggct
      421 ggggcgtagg cgagttgatc gcgaccatgg agtcctcttc gggcacggtt acctcttgag
      481 cgcgaaccgc gcggtgaaga aacgcgcgac gagcgtcacg atgaacgtga ccatcatcag
      541 cgtcagcgcg gcgccccacg cgcggtcctg cgcgggcgcg aatgccgaca ccgcgttgct
      601 gaagatctgc agtgacagcg ccgtgttcgc ctggctgaac aggtgcgggt tgaatgcggt
      661 cgcggcgccg acggtgaaca gcagcggcgc ggtctcgccg gcggcgcgcg cgatcgcgag
      721 cagcgatccg gagacgatgc cgggcagcgc cgcgggcagc acgacggtga gcacggtgcg
      781 gctcttcgtg gtccccagcg cgtaggctgc ctcgcgcagc tgcggcggca cgagccgcag
      841 catctgctcg gtggaaccga tcaccaccgg cagcatcagg caggcgaggg ccagcgcgcc
      901 gccgaacgcc gagtagccca ggctgagggt ccacatcagg tagatgaaga gccccatgac
      961 gatcgacggg acgccggtca tcaccgtggc catgaaccgc accagcctgg cgaacttgcc
     1021 ggccccgccg tactcgtgga ggtacaccgc gccgaggatt cccagcggca ccgcgatcaa
     1081 cgtcgcgccg cccgtggcca gcaaggtgcc gacgatcgcc gggcccatgc cgggaccggg
     1141 caagcgcgag atccggggga tcgtctcggt gaagaagctc gggaagtcct tgaacgcggt
     1201 ggaaccgccg cggacgatca cggaccacag caccgcgacc agcgggatcg cgaccagcac
     1261 gagtgccgcg atcatcagtc cgagcatcag gcggttcttg acggtgcgcc acgacggcct
     1321 ggcgcgcagg tcgttgcgcc gctgccggtt gacggtggat tcccggaagg cagatgcggt
     1381 ggtcatgagc cccggctccg cttcatcgag cgctgcacga tcgagttcgc gatcaggttg
     1441 acgatgatgg tgatgatgaa cagcacgacc gcgagcgcga tcagcgccgc cttgtggtgc
     1501 tcgtcggcct cgccccactc gttggcgatc accgccggca tcgagttgcc gggcgcgagc
     1561 gggttgatcg tgatctggcc gagcgccgag ccgatcacga gcgccgcggc gatcgtttca
     1621 cccatcgcgc gacccaggcc gagcatcacc gccccggtga tcccgccctt gctgtgcggc
     1681 agcaccgagg cggtgatcat ctcccagcgg gtcgcgccga gcgccagcgc cgcttccttc
     1741 tcggacgccg gcgtggtctc gatgacctcg cgcgcgagcg aggtgatgat cgggatgatc
     1801 atcacggcga ggatcacacc ggcggtgagg aacgatcggc tcagtccgaa gtgctgggac
     1861 aggacgagca cgccccacaa gccgaacacc accgagggaa ccacggcgag cagatcgagc
     1921 aggtagacca gcggcgtctt gagccacggc ggcgcgacct gggtgatgaa cagcgcgacg
     1981 ccgaggctga ccggcaccgc gaggatcacg gcgatcgccg cggtgtacag cgtgccccag
     2041 atgaacggca gcgcgccgaa ccggccgtcg ggcgccgacc accgggttga cgtgaagaac
     2101 tccagtccca gcgccggcca tgcgcgcagg gtcatccgga tcgcgatcat tccgagtacg
     2161 agcagcacga gcccggcggc ggcgatcgcg atcccgcgga acgcacggtc ggcgtactgc
     2221 ttgtgaccgc ggagctcgga cagctgcatc agggaacctg gatcttgtcg agctgcgcga
     2281 ccgccttgtc gtcgaggctc ttgggcagcg gcgcgaagtc gatgtccttg acctgcgcct
     2341 gcgcgtcggt cagcatgtgc ttgaagtacg ccttgaccgc ggcgcccttg gccttgtcgg
     2401 tctgcttggc gtacgcaacg caccaggtcg gcgctgtgat cgggtaggcc gcgtcgccct
     2461 tcgcgttgac cgatacgtag gtcaggttgt ccttgacgtc gatgccatcg ccggcagccg
     2521 atgccgacgc cgagctcggc tcgatgaact tgccggcctg gttcttcacc gaagcgaact
     2581 tgaggccggc tgccttggcg tccggcagat cgacatagcc gatcgcaccg ggcgtcgact
     2641 tcacgatctg cgcgacgcca ccgttgccgt taccggcctg ggtgtccgcc ggccactcca
     2701 ccgtcgagcc gctcttgagc ttccactgat ccggggccgc cgtgttgagg tacaaggtga
     2761 actgctcggt ggtgcccgag ccgtcggagc gatgggccac gacgatgtcg gtggcgggca
     2821 gcttcacgcc ggggttgtcc gccgcgatgt ccttgtcgtc ccacttcttg atctcgcgct
     2881 ggaagatctt tgcgatcgtg ggggccgaca gctggagctt gtcgacgccg tcgagtttgt
     2941 agctgaccgt gatcgcaccg agcaggatcg ggaagtacag gaacgggccg cccttggcct
     3001 tggccgcgtc ggcctccttg aacaggccgt cggtgcaccc gtaatcggtg accatgtcgg
     3061 agaagtcttg acgccccttg cccgagccac ccgcgccgta gttcaccttc acgtccttgt
     3121 tgaccttcga gaacccctcg atggcgagct cctggaattg cttctggaag gtcgacccgc
     3181 tgccgttgat cgtgaccgga ccggtttccg ggggcttggc cggacccttg tcctccttgg
     3241 tcatcgtagc ggtgctgccc ccgctctcat ccttcttctt gttgcagccg cccacgcaca
     3301 gtgccgccag ggcggagaac gcgatcatct tgtacatgcc atgaccgtag acagccttcg
     3361 tgacgcatct gcgcaccgtc tgtgaccgga ctgtgacggc cggccgggag tgctggctca
     3421 ccgttccatc ggtgggtatc cgcgcccggc gtgcgccggg gcgacgcgcg gcgtcgcgca
     3481 ggcagccgcc ctgaatctcg ctcgcgggag cggcggtcag ttttgcggga ggatcggccg
     3541 gtgccggacg tcggtggcct tgaccatgta gaccacctcc tccgcgaggt tcttgacatg
     3601 gtcgccgacc cgctccaggt agcggcacac cgaggtcagc ggcagcacgc gggtgacggt
     3661 ggccgggtcg gtcgcgacgt gcgcgatcag ctcggcgaac aggctggcgt tgagcttgtc
     3721 gatctcggcg tcggcggtga tcaccgcggt cgcgctctcg gcgtccttgc ggacgaacgc
     3781 gtcgagcgcg gcctgcaggt tcttctggac cagcgtggcg agcttgctga ggtcgatccg
     3841 cggctccagg ctgggcagcc ggttgagctc cagggcccgc ttggcgatcc cgcccgcgag
     3901 gtcgccgatc cgctcgaggt cgaccacgaa cttgagcgcc atggtgatga accgcaggtc
     3961 ggaggccacc ggctggcggg tcgcgaggat ctgcagcgcg agctcgtcga cgtcgttctc
     4021 gtcgcgatcg atctgggagt cgttccggat cacgtcgcgc gcgagctcgt cgtccttgtc
     4081 ggcgagcgcc ttcatcgcga gcgcgatctg gcgcgccgcg cgctcgccca tcgtggccag
     4141 ccgctcgcgc agcgtgcgca gctcttgttc gaagtccttg ctggtgtgcg ccgtcatggt
     4201 ggtgtcccca tgttgctcgc ccggggcagg cgaagccaga agatgctgcc gtgcggcagg
     4261 ttgggctcga ccccgacctc gccgtccatg ctctcgacca ggtgcttgac gatcgacagg
     4321 cccaggccgg tgccgccggc ctcgcgcgac cggctggggt cggcgcggta gaaccgctcg
     4381 aacacccggg tacggtgctt gtcggcgatg cccgggccgt cgtcgcggac ctcgatccgg
     4441 accccttcgg gccgcacccc gtccgccgcg ccgtcgccga ccggccgggc ctcgacccag
     4501 acgttgccgt cgggccggcc gtacttgacg ccgttgtcga tcaggttgac caggatgtgg
     4561 tcgagcgcct tgggatcggc gcggaccgcg aggtcggtcg ggatgtcgac ccggaccgtg
     4621 acgttgcgct gcgccgcctg gggctcgacc gcgctcagcg actgctcgat caccggtcgg
     4681 accgccacgt cggcgagctc caggcggtac tggccggcgt cgagccgcga caggtcgagc
     4741 aggtcggcga ggatgcgggc gagccgctcg gcgttgcggt gcaggccgtc gatcagccgg
     4801 ccggcgatcg ccggatcgtc cttggcgccc gccatcaggg tctcggcgtt ggcgcggatc
     4861 accgcgaccg gggtgcgcag ctcgtgcgag acgttggcga cgaagtcgcg ccgcacggtc
     4921 tcgagccggc gcaccgtggt gacgtcctcg agcagcagca cccggccgtc gccgccccac
     4981 ttgcgcgcgg tccggatcag cgcgcgcggc gtgttgggca gcgagacctc ggcggtcgcc
     5041 gcgacgtcgc tgtcgatcag atcgaacagc tcggggaccc gcacgaagtc gatcagcggc
     5101 tcgccgaccg gcgagctggt gaccccgagc atcttgcgcg cggcgtcgtt gagcagctcg
     5161 atccggtgct cgctgtcgac cgcgatcacg ccctgggtcg acccgtcggc gaccgcggcg
     5221 agcagcatgc gctcgcggac cagcgcgcgg tggctcttgt cggcgtcgtc ggccaggaag
     5281 ttgatccact cggtgagctc gcggagctcg tcgccgaccg cgccgtcggg gcggatccgc
     5341 cggctcggat cgcgcaccac gtcgcgcatc gacttgacca cgcggcggat cgcaccgcgc
     5401 aggatcaccg tcaccaccca gccgatcacg atcgagaccg cgaacgcggc gatcgcgatc
     5461 gacagcgcgt cggaggccag ctgcgggtgc agcaccgcgg tcgcgagcgt accggcgacg
     5521 atcgagacca ggagtgcgag gccgagcagg cgaaggcgga cgcgctggat caagcggtgg
     5581 cctcgtcggg ctgatccttg aaccggtagc cgacgccgcg cagggtctcg atgtaggcac
     5641 cggcgtcgcc cagcttctcg cgcagccgct tgacatgggt atcgaccgtg cgggtggtga
     5701 catcggcctc gatgccccac acgtcgctca gcagggcgtc gcgggtctgg acccggccgc
     5761 gccgcgacag gaacgcgtgc agcaggcgga actccagcgc ggtcaggccg atctcggcgt
     5821 cgtcgaccca cgcccggtgg gcgtcgcgat cgatcttgag ccggccgaac cggatcaggg
     5881 acggctcgcc ctcgacgcgg tgcgaccggc ggagcagcgc gcggatccgg aggatcagct
     5941 cgcgcacgct gtacggcttg accacgtagt cgtcggcgcc gacctcgaac ccgaccaccc
     6001 ggtcgatctc ctcgcccttg gccgtgcaca tcacgaccgg gatgtcgcgc gtgcgctcgc
     6061 cttcgcgcag cttgcggcag atctcggtgc ccgacaggtc gggcagcatc agatcgagca
     6121 cgatgacgtc cgggagtgga tcggccgtcg cgctcgcgag gccctggcgg ccgctatgcg
     6181 cgagccgcac cgtgaagccc tcggcgcgca agttgtactc gagcgtggtg gcgaggtcga
     6241 cctcgtcctc gatgaccagt acgagcatgg gggagcctta gcatgctctc ggtgacgggg
     6301 gtgtgacgac gagattccgt cccggtaacg gataccggac ccggggggcc gatcccgtac
     6361 cggtcggcgc cggtacgatc tgtcgcgggt ccgtcacgac cgcgtcacgg ccgctggtat
     6421 acgtactgcc cgatgcagcg cctatcgata tccatctttt cgctcgtcgc gctggccg
//
LOCUS       DASVOR010001624         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_395865,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001624 DASVOR010000000
VERSION     DASVOR010001624.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395865"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VF516_30430"
     CDS             complement(<1..297)
                     /locus_tag="VF516_30430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112103.1"
                     /translation="MTTAALLDEVIETLYARHTTDPEAASIARREYEDRRGKVHQEDE
                     LWEPWSAAFVEWYVVERVAPGKTHPPAVATYRELEAAGDPRAEAARALVTSQRSL"
     gene            complement(294..1475)
                     /gene="carA"
                     /locus_tag="VF516_30435"
     CDS             complement(294..1475)
                     /gene="carA"
                     /locus_tag="VF516_30435"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEX8112104.1"
                     /translation="MSEPSNDASKPGGAGEAARWPAALVLENGNVYRGDGFGARVESI
                     GEVVFNTSITGYQEIISDPSYCGQIVVMTATQIGNVGVNRLDVEARRVACAGFAIREL
                     SPIPASWRAEADLPGWLAGSGVPGIDGIDTRSLTRCLRDSGAMRGAITPHVGELAAVL
                     DRVRAAPKMDGLDLVPRVTCAERYEWIEPTWRADDDGRALEPPPIAHHVVAYDFGIKE
                     NILRQLRAVGCRVTVVPATTPARVALALGADGFFLSNGPGDPAVVAYGIAAARELCAS
                     GKPVFGICLGHQILGLALGGKTRKLPFGHHGANHPVLDRTTGKVEITSQNHGFVVDPA
                     SLPSGVQLTHENLFDHSNEGIAVEGKPIFSVQYHPEASPGPHDANYLFARFRDYLRKA
                     T"
     gene            complement(1472..>2137)
                     /locus_tag="VF516_30440"
     CDS             complement(1472..>2137)
                     /locus_tag="VF516_30440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8112105.1"
                     /translation="CAESAMVARDLEIVEWTGARYHVAHASTARTVALVRDAKRRGLP
                     VTCEVTPHHLALTDEACAHYDTRTKVMPPLRTPADQEALLEGLIDGTIDCIATDHAPH
                     SPVEKDVEFECAAPGMLGLETALPIILDFVRMGSLDERRAIAALTGAPARAFGLPGGT
                     LAVGAVADVCVIDPERPVTITAEELASKSKNTPLLGQTLAGRAVLTLVEGRCVFDLDG
                     RTR"
BASE COUNT          266 a          811 c          757 g          303 t
ORIGIN      
        1 caggctgcgc tgcgaggtca ccagcgcccg cgccgcctcg gcgcgcggat cgcccgccgc
       61 ctcgagctcg cgatacgtcg ccaccgccgg cgggtgggtc ttgccgggcg cgacgcgctc
      121 gaccacgtac cactcgacga acgccgcgct ccacggctcc cacagctcgt cctcctggtg
      181 gaccttgccg cggcggtcct cgtactcgcg gcgcgcgatc gacgccgcct ccggatcggt
      241 ggtgtggcgg gcgtacagcg tctcgatcac ctcgtcgagc agcgcggccg tggtcatgtc
      301 gccttgcgca ggtagtcgcg aaaccgcgcg aacaggtagt tcgcgtcgtg cggcccgggg
      361 ctggcctcgg ggtggtactg gacgctgaag atcggcttgc cctcgaccgc gatgccctcg
      421 ttgctgtgat cgaacaggtt ctcgtgggtc agctgcacgc ccgacggcag cgacgccgga
      481 tcgacgacga agccgtggtt ctgcgacgtg atctcgacct tgccggtggt ccggtcgagc
      541 accgggtggt tggcgccgtg gtggccgaac ggcagcttgc gggtcttgcc gccgagcgcc
      601 agcccgagga tctggtggcc gaggcagatc ccgaacaccg gcttgccgct cgcgcacagc
      661 tcgcgggccg ccgcgatgcc gtacgcgacc accgccggat cgcccgggcc gttggacagg
      721 aagaacccgt cggcgccgag cgcgagcgcg acccgcgccg gcgtggtcgc cggcaccacg
      781 gtgacgcggc agccgaccgc gcgcagctgg cgcaggatgt tctccttgat cccgaagtcg
      841 taggcgacca cgtggtgcgc gatcggcggc ggctcgagcg cgcggccgtc gtcgtcggcg
      901 cgccaggtcg gctcgatcca ctcgtagcgc tcggcgcagg tcacccgcgg cacgaggtcg
      961 aggccgtcca tcttcggcgc ggcgcgcacg cgatccagca ccgcggcgag ctcgccgaca
     1021 tgcggcgtga tcgccccgcg catcgcgccg gagtcgcgca agcagcgggt cagcgagcgg
     1081 gtgtcgatgc cgtcgatccc cggcacgccg ctgcccgcca gccagcccgg caggtcggcc
     1141 tcggcgcgcc agctcgccgg gatcggcgac agctcgcgga tcgcgaaccc ggcgcacgcg
     1201 acccggcgcg cctcgacgtc gagccggttg accccgacgt tgccgatctg ggtcgccgtc
     1261 atcacgacga tctgcccgca gtacgacggg tccgagatga tctcctggta gcccgtgatc
     1321 gacgtgttga acacgacctc gccgatcgat tcgacccgcg cgccgaaccc gtcgccgcgg
     1381 tagacgttgc cgttctccag gaccagcgcc gccggccagc gggccgcctc gcccgccccg
     1441 cccggcttgc tcgcgtcgtt gcttggctcg ctcaccgcgt cctcccgtcg aggtcgaaca
     1501 cgcaccggcc ctcgaccagg gtcagcaccg cgcgccccgc cagggtctgg ccgagcagcg
     1561 gcgtgttctt cgacttgctg gcgagctcct cggcggtgat cgtcaccggc cgctcggggt
     1621 cgatcacgca gacgtcggcg accgcgccga ccgccagcgt cccgccgggc agcccgaacg
     1681 cccgggccgg cgccccggtc agcgccgcga tcgcgcgccg ctcgtcgagc gagcccatcc
     1741 gcacgaagtc gaggatgatc ggcagcgcgg tctcgagccc gagcatgccc ggcgccgcgc
     1801 actcgaactc gacgtccttc tcgaccggcg agtgcggcgc gtgatcggtg gcgatgcagt
     1861 cgatcgtccc gtcgatcagg ccctcgagca gcgcctcctg atcggccggc gtgcgcagcg
     1921 gcggcatcac cttggtgcgg gtgtcgtagt gcgcgcaggc ctcgtcggtc agcgccaggt
     1981 ggtgcggcgt gacctcgcag gtcaccggca gcccgcgccg cttggcgtcg cgcaccagcg
     2041 ccaccgtccg cgccgtcgac gcgtgcgcga cgtggtagcg cgcgccggtc cactcgacga
     2101 tctccaggtc gcgcgcgacc atcgcgctct cggcgca
//
LOCUS       DASVOR010001625         1749 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_162540,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001625 DASVOR010000000
VERSION     DASVOR010001625.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1749
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162540"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF516_30445"
     CDS             complement(<1..498)
                     /locus_tag="VF516_30445"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB small GTPase domain-containing protein"
                     /protein_id="HEX8112106.1"
                     /translation="MTQASCHGPAVLHDPVPTRPQLVLAGNPNVGKTTLFNALTRSSA
                     KVSNYPGITVEVACGALTLPGGRIADLRDLPGTYSINARSAEEQIALDALIGLDGAPV
                     PDAVIVCVDATQVARAAYLLLQCQELGARCVVALTMVDEAGAAAPDPRALTGALGCEV
                     VAVTAR"
     gene            complement(495..722)
                     /locus_tag="VF516_30450"
     CDS             complement(495..722)
                     /locus_tag="VF516_30450"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HEX8112107.1"
                     /translation="MTSLAETPLGRAVAVHAVDGNRAFRRRLLEMGLVPGTTVRVVTV
                     APLGDPLRIEVRGGQWSIRRAEAAQIQVAPR"
     gene            895..1449
                     /locus_tag="VF516_30455"
     CDS             895..1449
                     /locus_tag="VF516_30455"
                     /inference="COORDINATES: protein motif:HMM:NF025637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEX8112108.1"
                     /translation="MNSAQTLLLNQGFEPIKVISWQRAISLLFLGKVEVLEEYDRDIR
                     SVNLIIKVPAVVRLLRAFRRHARPVKFSRVNIYARDNYKCQYCGKKASISELTYDHVV
                     PRSQGGMTEWTNIVTCCYLCNRKKGGRTPREAGMALLGAPSQPNWVPAVAIRISLRSI
                     PDAWRDYLYWTGELDADPPDPGDG"
     gene            1558..>1749
                     /locus_tag="VF516_30460"
     CDS             1558..>1749
                     /locus_tag="VF516_30460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112109.1"
                     /translation="MVDRRVIERAAGALAGRAIAAARALTRDGHAIDDHQVVVERVAY
                     AATEARVIAELATLPDELGE"
BASE COUNT          281 a          595 c          633 g          240 t
ORIGIN      
        1 ccgcgcggtg accgcgacca cctcgcagcc cagcgcgccg gtgagcgcgc gcggatcggg
       61 cgccgccgcg ccggcctcgt cgaccatggt cagcgcgacg acgcagcgcg cgccgagctc
      121 ctggcactgc agcagcaggt aggccgcgcg cgcgacctgg gtcgcatcga cgcagacgat
      181 cacggcgtcg ggcaccggcg cgccgtccag gccgatcagg gcgtcgagcg cgatctgctc
      241 ctcggcgctg cgcgcgttga tcgagtaggt gccgggcaga tcgcgcaggt cggcgatccg
      301 gccgccgggc agggtgagcg caccgcacgc gacctcgacg gtgatgccgg ggtagttgga
      361 gaccttggcc gacgacctgg tcagcgcgtt gaacagcgtg gtcttgccga cgttggggtt
      421 gccggcgagc acgagctggg gccgggtcgg gaccggatcg tggagcacgg ccgggccgtg
      481 acaggacgcc tgggtcatcg cggcgcgacc tggatctgag ccgcctccgc acgccggatc
      541 gaccactgcc cgccgcggac ctcgatgcgg agcggatcgc cgagcggcgc gaccgtcacc
      601 acccggaccg tggtccccgg gaccaggccc atctcgagca gccggcggcg gaacgcgcga
      661 ttcccatcga cagcgtggac cgccaccgcg cggcccagcg gagtctcggc gagcgaagtc
      721 acccgagaac tgtaccattt ctgaaaatgg ctttcaaatc tcggaaactt gagaccggat
      781 cgacaccggg ccgttgcgaa tcgacgtcac accgacaagt aagccgggat ccgcccgccg
      841 gtcggggggt gtcgcggacg gctgacgcga tgctacattt gcacagggtt ctcgatgaac
      901 agcgcgcaga ctttgctgct caatcaggga ttcgaaccga tcaaggtcat ctcctggcag
      961 cgggcgatca gcctgctgtt tctcgggaag gtcgaggttc tcgaggagta cgaccgcgac
     1021 atccgatcgg tgaacctcat catcaaggtt cccgcggtcg tgcgcctgct ccgcgcgttc
     1081 cgccgccatg cgcgcccggt gaagttctcg cgggtcaaca tctacgcccg cgacaattac
     1141 aagtgccagt attgcgggaa gaaagcgtcg atcagcgagc tgacctacga ccacgtcgtg
     1201 ccgcgctcgc agggcgggat gaccgagtgg accaacatcg tgacgtgctg ctacctgtgc
     1261 aaccgcaaga aggggggccg cacgccgcgg gaggccggca tggcgctgct cggggcgccg
     1321 agccagccca actgggtgcc ggcggtcgcg atccggatct cgctgagatc gatcccggat
     1381 gcctggcggg actacctgta ctggaccggc gagctggatg cggatccgcc ggatcccggc
     1441 gatggctagc ggcggacgcg gcggagcgat ggccgccggg tctactcgcc ccgcagaggc
     1501 cgacggaacc cggcgcgagg ggtcgtgcgc cgcgcggtcc cgcgataaca agtcgccatg
     1561 gtcgatcgac gcgtgatcga gcgggccgcc ggggcgctcg ccgggcgcgc gatcgcggcc
     1621 gcgcgcgcgc tgacccgcga cggccacgcg atcgacgatc accaggtcgt ggtcgagcgg
     1681 gtcgcctacg ccgccaccga ggcgcgcgtg atcgccgagc tcgccacgct acccgacgag
     1741 ctgggtgag
//
LOCUS       DASVOR010001626         6834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_46170,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001626 DASVOR010000000
VERSION     DASVOR010001626.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46170"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /locus_tag="VF516_30465"
     CDS             <1..685
                     /locus_tag="VF516_30465"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8112110.1"
                     /translation="LRGVDVRVSRGSTIAMVGATGSGKSTVIKLLTRLYERDRGAIRI
                     DGVDVRDIALAELRRRITVVSQDVILFAGTLEDNITLGKPYARDQLETAIRRVGLDRA
                     LVRRGTGLETKVAERGANFSAGERQLVAFARALLRDPEILVLDEATAHVDPEAEELIE
                     KGVAELMKGRTTLVIAHRLSTIRNADLIVVMDKGQVIEQGKHDELIARGGCYAALERT
                     FRRQTADPT"
     gene            884..3259
                     /locus_tag="VF516_30470"
     CDS             884..3259
                     /locus_tag="VF516_30470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112111.1"
                     /translation="MSPASIDLLRHAVGAMADGAIVADTRTGEVFINAAAREMLGIPA
                     DAQIDTTYLKDTVGFYPFDLAAGDQAIREEVRIGERVLHSVVTPLRDGAAALGAIVLL
                     RDLGDTAEVARRRAEFAQVMSHELRTPLTSIAGALDIVLSGYAGPLSDPQLRYVDMAR
                     QATTRMNQLVDQLLELARAQAGSITVAATPVQLDRLAREVIDRYRAAAATKHLTIVLG
                     ASAEDISILGDPERLAQVLGNLLSNAIRFAPNGGVIDVQVFGPPLTEDAVGVSVFNSG
                     EPIAAEDRERVFEPFSASSRKVGGTALGLSISRTIIEAHGGRIWVESEPDGTKFVFTL
                     PSTAMPEKLAATPLEAPPRRRGLGEGSCALVCDDDPRRALLLKGLLMSLSDHVLVAHD
                     VDTALAISRAEHPALAVVAGAMSDAPALLAILEHDPDTRKTAVMVVGDAERRADLISA
                     GADDVIEFPIQPAVFREACLRLVESGRREAPRVLVVDDDGSIRSICREVLELGGYQVR
                     DAGSATSALAEARRFRPDMILLDVLMPGIDGYRCAEMLRADPGVGMAPIMFLSARADT
                     ADKVRAFRSGAEDYMVKPFDADELLARVGKALDRQARELGASPTTQLPGADAIQAEIE
                     RRLSIADTDAVACYLDLDNLKAFNDYYGYAKANAVIRQTGDVIRHVVTRSGVTGDFIG
                     HIAGDDFVFITSLDKVDEVCRGICERFDHLIRLYYDPADRARGYIETKDRFGVQRRFP
                     IMSVSIAAISIARAKSYAALAELAAVGKRAAKDMPGSTYVRDGQTLLAATA"
     gene            complement(3266..4354)
                     /locus_tag="VF516_30475"
     CDS             complement(3266..4354)
                     /locus_tag="VF516_30475"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEX8112112.1"
                     /translation="MSTRIGILTGGGDCPGLNGVIRAVTLHARQTFGWEVLGIKNGFE
                     GLYEEEYVELTSHSVQHSLQRGGTMLGSSNRANPFAYPVRLPDGRIEIHDVSTRCMSN
                     LAKLDLHGLIVVGGDGSMSFAHKFIQRGATRIVGVPKTIDNDLEATDYTVGFQTAVEV
                     VVDAVDRLHTTAESHERIMIVEVMGRYAGWIALTAGLAGGAHVVLIPEIDYDPMRVIE
                     AFHRRHGRGVSYSIAVVAEGAKPRGGALSVVTAGDVTKQEKLGGAGTHLAEVIAANTE
                     YEVRTTVLGHIQRGGAPCAFDRELATRFGVKAVDLIAEGKFGHVAAVESGHLVAKPMD
                     KAVKKLKLVDPQGEQVRTAKAIGIEFGD"
     gene            complement(4437..4931)
                     /locus_tag="VF516_30480"
     CDS             complement(4437..4931)
                     /locus_tag="VF516_30480"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HEX8112113.1"
                     /translation="MPLLDGLQVVRSKIHGYGVVATRPYRAGEILCYGDGLLYTADAD
                     FDDTYALVLPGEDTATGDPLFWDLVCQTRWFNHSCDPNTEVMLKWDPEAEQMTAWWVA
                     LRDIAVGEEITYDYAFVADVAEPCRCGAAICRGVIVDDDPDNVARLPDHLRALLRAPA
                     RAAS"
     gene            5347..>6834
                     /locus_tag="VF516_30485"
     CDS             5347..>6834
                     /locus_tag="VF516_30485"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEX8112114.1"
                     /translation="MSKSLVPERGVRKTAATPQPKLARGTRDISPATLKAARGTRDIS
                     PLVPLDSDDDTIVVDTDPNLPEDLGLEAEVGDADVGEGGESSEVTETQAASIDARMLE
                     LGKRRFGITAFRPGQALAIRNVLAGIDTLAIMPTGAGKSLCYQLPALELDGVSLVVSP
                     LIALMKDQHDKLFELGIEVLRLDSTLSPRDEQAALSRLSENRPCIAYVTPERLGEPRF
                     RERLAAVRVALFVVDEAHCISQWGHDFRPAYLGLGEAVRALGRPPVLALTATAPPKVK
                     DDILAQLQLSPDDASVIDIGLNRPNLRYHVIKASSERKKQALLLRLIEKQAGCGIIYA
                     ATVKTVDALADFLWSQGVECGRYHGRMRAKDRERVQSAFMEHSEPRLMVATNAFGLGV
                     DKQDLRFVIHYNFPGSLESYYQEAGRAGRDGVPADCVLLYQPEDKRIQSFFLGGRYPT
                     PEQTRAVAEALVHVTQGRTVTPGQAQGTGDDIDPDVFIAVKDIAER"
BASE COUNT         1020 a         2469 c         2349 g          996 t
ORIGIN      
        1 gctgcgcggc gtcgacgtcc gggtcagccg cggctcgacg atcgccatgg tcggcgccac
       61 cggatcgggc aagtcgaccg tgatcaagct gttgacccgg ctgtacgagc gcgaccgcgg
      121 cgcgatccgg atcgacggcg tcgacgtccg cgacatcgcg ctcgccgagc tgcgccgccg
      181 gatcaccgtg gtgtcgcagg acgtgatcct gttcgccggc acgctcgagg acaacatcac
      241 gctcggcaag ccgtacgccc gggaccagct cgagaccgcg atccggcggg tcgggctcga
      301 ccgcgcgctg gtgcgccgcg gcaccggcct cgagaccaag gtcgccgagc gcggtgcgaa
      361 cttctcggcc ggcgagcgcc agctggtcgc gttcgcgcgc gcgttgctcc gcgaccccga
      421 gatcctcgtg ctcgatgagg ccacggcgca cgtcgacccc gaggccgagg agctgatcga
      481 gaagggcgtc gccgagctga tgaaggggcg taccacgctg gtcatcgcac accggctatc
      541 cacgatccgc aacgccgatc tcatcgtggt gatggacaag ggtcaggtca tcgagcaggg
      601 caagcacgac gagctgatcg cccggggtgg gtgctacgcg gcgctggagc ggacgttccg
      661 ccgccaaact gccgatccga catgacccgg cccccctcac cggctccatc ggcccgctcc
      721 tccggcccgc tcctccggcc cgctcccccc tgacgcatcc cggcccgtct cggcccaacc
      781 ccgggtcagc tccggcatct cgggcgctcc tcactaccta cccacacagc cgtcccgccg
      841 cggacctgtg ctttgaccct gatttgggta tgattgctaa cagatgtccc ccgcgtcgat
      901 cgacctgctc cggcatgcgg tgggcgcgat ggcagatggt gccatcgtcg ccgacacccg
      961 cacgggagag gtgttcatca atgccgcggc gcgcgagatg ctgggaattc cagcggacgc
     1021 gcagattgac accacgtacc tgaaggacac cgtcgggttt tacccgttcg acctcgccgc
     1081 cggggatcag gcgatccggg aggaggtccg catcggcgag cgcgtgctgc actcggtcgt
     1141 gaccccgctg cgcgacggcg cggccgcgct cggcgcgatc gtcctgctac gcgacctggg
     1201 agacaccgcg gaggtcgccc gccggcgcgc cgagttcgcg caagtgatgt ctcatgaact
     1261 tcgaacaccc ctgacgtcga tcgccggggc gctcgacatc gtgctgtcgg gctacgccgg
     1321 gccgctgtcg gacccgcagc tgcgctacgt cgacatggcg cgccaggcga cgacccggat
     1381 gaaccagctc gtcgatcagc tgctcgagct cgcgcgcgcc caggccggct cgatcacggt
     1441 cgcggcgacc ccggtccagc tcgaccggct cgcccgcgag gtgatcgacc gctaccgcgc
     1501 cgcggcggcg accaagcacc tgacgatcgt gctcggcgcg tccgccgagg acatctcgat
     1561 cctcggcgat cccgagcggc tggcccaggt gctcggcaac ctgctgtcca acgcgatccg
     1621 gttcgcgccc aacggcggcg tgatcgacgt ccaggtgttc gggccgccgc tgaccgagga
     1681 cgcggtcggc gtgtcggtgt tcaactccgg cgagccgatc gcggccgagg accgcgagcg
     1741 cgtgttcgag ccgttctcgg cgtcgagccg gaaggtcggc ggcaccgcgc tcggtctgtc
     1801 gatctcgcgc acgatcatcg aggcccacgg cgggcggatc tgggtcgagt ccgagcccga
     1861 cggcaccaag ttcgtgttca ccctgccgtc gaccgcgatg cccgagaagc tggcggcgac
     1921 cccgctcgag gcgccgccgc gccggcgcgg cctgggcgag ggctcgtgcg cgctggtctg
     1981 cgacgacgat ccgcggcgcg cgctgctgct caagggcctc ttgatgtcgc tcagcgacca
     2041 cgtgctggtc gcccacgacg tcgacaccgc gctcgcgatc agccgcgccg agcaccccgc
     2101 gctcgcggtc gtcgccggcg cgatgtccga cgccccggcg ctgctcgcga tcctcgagca
     2161 cgatcccgac acccgcaaga ccgcggtcat ggtggtcggc gacgccgagc gccgtgccga
     2221 cctgatctcg gccggcgccg acgacgtcat cgagttcccg atccagcccg cggtgttccg
     2281 cgaggcctgc ctgcggctgg tcgagtccgg tcggcgcgag gcgccgcgcg tgctggtggt
     2341 cgacgatgac ggctcgatcc gctcgatctg ccgcgaggtc ctcgagctcg gcggctacca
     2401 ggtccgcgac gccggctcgg cgaccagcgc gctcgccgag gcccgccggt tccgccccga
     2461 catgatcctg ctcgacgtgc tgatgcccgg catcgacggc taccgctgcg ccgagatgct
     2521 gcgcgccgac cccggggtcg gcatggcgcc gatcatgttc ctgtcggcgc gcgccgacac
     2581 ggccgacaag gtccgcgcgt tccgctcggg cgccgaggac tacatggtca agccgttcga
     2641 cgccgacgag ctgctcgccc gggtcggcaa ggcgctcgac cgccaggccc gcgagctcgg
     2701 cgcgtcgccg accacccagc tgccgggcgc cgacgcgatc caggccgaga tcgagcgccg
     2761 gctctcgatc gccgacaccg acgcggtcgc ctgctacctc gacctcgaca acctcaaggc
     2821 gttcaacgac tactacggct acgccaaggc caacgcggtc attcgccaga ccggcgacgt
     2881 gatccgccac gtcgtcaccc gcagcggggt cacgggcgac ttcatcggcc acatcgccgg
     2941 cgacgacttc gtgttcatca ccagcctgga caaggtcgac gaggtctgcc gcggcatctg
     3001 cgagcgcttc gaccacctga tccgcctgta ctacgacccc gccgaccgcg cgcgcggcta
     3061 catcgagacc aaggaccgct tcggcgtgca gcgccggttc ccgatcatga gcgtctcgat
     3121 cgccgcgatc tcgatcgcgc gcgccaagtc gtacgccgcc ctcgccgagc tcgccgcggt
     3181 cggcaagcgc gccgccaagg acatgccggg ctcgacctac gtccgcgacg gccagaccct
     3241 gctcgccgcg accgcgtgac cccggctaat ccccgaactc gatgccgatg gccttggcgg
     3301 tgcggacctg ctcgccctgg ggatcgacca gcttgagctt cttgaccgcc ttgtccatcg
     3361 gcttggcgac caggtggcca ctctcgaccg cggcgacgtg gccgaacttg ccctcggcga
     3421 tcaggtcgac cgccttgacc ccgaaccggg tcgcgagctc gcggtcgaac gcgcacggcg
     3481 cgccgccgcg ctggatgtgg ccgagcacgg tggtgcggac ctcgtactcg gtgttggccg
     3541 cgatcacctc ggcgaggtgg gtaccggcgc cgccgagctt ctcctgcttg gtcacgtcgc
     3601 cggcggtgac caccgacagc gcaccgcccc tgggcttggc gccctcggcg acgaccgcga
     3661 tcgagtagct gacgccgcgg ccgtggcggc ggtggaacgc ctcgatgacg cgcatcggat
     3721 cgtaatcgat ctcggggatc agcacgacgt gggcgccgcc ggcgaggccg gcggtgagcg
     3781 cgatccagcc ggcgtagcgg cccatgacct cgacgatcat gatgcgctcg tggctctccg
     3841 cggtggtgtg cagccggtcg accgcgtcga ccacgacctc gacggcggtc tggaacccga
     3901 cggtgtagtc ggtcgcctcg aggtcgttgt cgatggtctt gggcacgccg acgatccggg
     3961 tcgcgccgcg ctggatgaac ttgtgggcga acgacatcga gccgtcgccg ccgaccacga
     4021 tcaggccgtg gaggtcgagc ttggcgaggt tggacatgca gcgggtggag acgtcgtgga
     4081 tctcgatccg gccgtcgggc agccgcaccg gatatgcgaa cgggttggcg cggttgctcg
     4141 agcccagcat cgtgccgccg cgctgcagcg agtgctgcac gctgtgcgag gtgagctcga
     4201 cgtactcctc ctcgtacagg ccctcgaagc cgttcttgat gcccaggacc tcccagccga
     4261 aggtctggcg ggcgtgcagc gtgacggcgc ggatcacgcc gttgaggccc gggcagtcgc
     4321 caccgccggt gaggatgccg atgcgtgtgc tcatcgccgg cgagtatcac cgattttgcg
     4381 agcgtcgcat cggcgcacac gcgggaccac gtagcgcggc cgcgcgctgc cgatcgctac
     4441 gaggcggcgc gcgctggcgc gcggagcagc gcgcgcaggt gatcgggcag cctggcgacg
     4501 ttatccggat cgtcgtcgac gatcacgccg cggcagatcg ccgcgccgca ccggcacggc
     4561 tcggcgacat ccgcgacgaa cgcgtagtcg taggtgatct cctcgccgac cgcgatgtcg
     4621 cgcagcgcga cccaccacgc ggtcatctgc tcggcctcgg gatcccactt cagcatcacc
     4681 tcggtgttgg gatcgcacga gtgattgaac cagcgggtct ggcacaccag gtcccagaac
     4741 agcggatcac cggtcgcggt gtcctcgccc ggcaggacga gcgcgtaggt gtcgtcgaag
     4801 tcggcgtccg ccgtgtacag caggccgtcg ccgtagcaca ggatctcgcc ggcgcggtac
     4861 ggccgggtcg ccacgacgcc atagccgtgg atcttggacc tcacgacttg cagcccatcg
     4921 agcagaggca tgggcagcat cttcatcaac cgtgcgcggc ttgcaaggcc gcgcgcgcga
     4981 tgtctttccc ggaacggcca atagcacccc gggcgatcgc cggccaggcc cgaaccagcg
     5041 gctgccgcgc gattccggaa tgccgccgcc cgaatgccgc cgcccgaatg ccgccgaatg
     5101 ttgcaggtga cgactgtggc gcgggcgcac gaggaggagg agtgtcattc gtctgtgacg
     5161 tgaacacgcg ctcttgcagc tgtgatggcg ctagcgattc tctggaccgg tgatgccgca
     5221 acacagtttc tcgacgggac tgaatgtagg tccgacgatc gacgccgcgc gaacaactgt
     5281 tacctaacga gatcgttcca acgtttcact tgcaggcacc ggcgttccag gcgacggtaa
     5341 ccgctaatgt ccaagagcct cgtgcctgag cgcggcgtac gaaagaccgc cgcgacgcca
     5401 cagccaaaac tggcgcgcgg gacccgcgac atatcgcccg caacgctcaa ggccgcgcgc
     5461 gggacccgcg acatatcgcc gctcgtcccg ctcgactccg atgacgacac catcgtggtc
     5521 gacaccgatc cgaacctccc ggaggacctg gggctcgagg ccgaggtcgg cgacgcggac
     5581 gtcggcgaag gcggcgaatc ctccgaggtg accgagaccc aggcggcgtc gatcgacgcc
     5641 cggatgctcg agctcggcaa gcggcggttc gggatcacgg cgttccggcc cggccaggcg
     5701 ctggcgatcc gcaacgtgct cgccggcatc gacacgctgg cgatcatgcc gaccggggcc
     5761 ggcaagtcgc tgtgctacca gctgcccgcg ctcgagctcg acggcgtcag cctggtggtg
     5821 tcgccgctga tcgcgctgat gaaggaccag cacgacaagc tcttcgagct cgggatcgag
     5881 gtgctgcggc tcgactcgac gctgtcgccg cgcgacgagc aggccgcgct gtcccggctg
     5941 tcggagaacc ggccgtgcat cgcgtacgtc acgccggagc ggctcggcga gccccggttc
     6001 cgcgagcggc tggcggcggt ccgggtcgcg ctgttcgtgg tcgacgaggc gcactgcatc
     6061 tcgcagtggg ggcacgactt ccggccggcc tacctcgggc tcggcgaggc ggtgcgcgcg
     6121 ctcggccggc cgccggtgct ggcgctgacc gcgaccgcgc cgcccaaggt caaggacgac
     6181 atcctcgccc agctccagct cagccccgac gacgcgtcgg tgatcgacat cgggctcaac
     6241 cggcccaacc tgcgctacca cgtgatcaag gcgtcgagcg agcgcaagaa gcaggcgctg
     6301 ctgctccggc tgatcgagaa gcaggccggc tgcggcatca tctacgccgc gacggtcaag
     6361 acggtcgacg cgctcgccga cttcctgtgg tcgcagggcg tcgagtgcgg ccgctaccac
     6421 ggccggatgc gggccaagga ccgcgagcgg gtccagagcg cgttcatgga gcacagcgag
     6481 ccgcggctga tggtcgcgac caacgcgttc ggcctgggcg tcgacaagca ggacctgcgg
     6541 ttcgtcatcc actacaactt ccccggctcg ctggagagct actaccagga ggccggccgc
     6601 gccgggcgcg acggcgtccc cgccgactgc gtgctgctgt accagcccga ggacaagcgg
     6661 atccagagct tcttcctggg cggccgctac cccacgccgg agcagacccg cgcggtcgcc
     6721 gaggcgctgg tccacgtgac ccagggccgc accgtgacgc cggggcaggc gcagggcacc
     6781 ggcgacgaca tcgatcccga cgtgttcatc gcggtcaagg acatcgccga gcgc
//
LOCUS       DASVOR010001627         6415 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_454824,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001627 DASVOR010000000
VERSION     DASVOR010001627.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454824"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..705
                     /locus_tag="VF516_30490"
     CDS             46..705
                     /locus_tag="VF516_30490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX8112115.1"
                     /translation="MRALVALFVFVAVLTAGCLRTTEFRCASNAECGAGGVCELVGFC
                     SVPNPECAGTGRSYSDSAGQGLSNTCVPAGAPGPGNDAGVDAPEDAMPIPKCPSGYAA
                     VAGSAHLYKALSGVTWDEAKMMCDLTSRAAYLAVPDDATELANLAIVAAAPLWVGIDD
                     KATSGMFVTQKGAPASFLPWGPGQPNLGPQNDCVTAISTTQIATEKCSTKHAAVCECE
                     P"
     gene            complement(844..1881)
                     /locus_tag="VF516_30495"
     CDS             complement(844..1881)
                     /locus_tag="VF516_30495"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX8112116.1"
                     /translation="MATHIAPPPALEPLEIHAEWRARDVADGSRWTYRLSDAEVAELE
                     AALAHARARCADVLDITREDFPLPTLAAKLAGFEDELINGRGFQLISGLPAERYSDAD
                     ASAIYWGIGMHLGTPWPQNQHGHLLGDVTDQGRSHVDPTSRGNQLGPVAMPYHSDGSD
                     LVGLMCLRKARSGGLSTVANAVLAHNEMVRTRPDLAAVLYQPMIYDFRGEQQPGRSPT
                     YTMPVFTRLGDRLFVRYIRPYIESARRHPGVPAVTAAEREAFDMLDRLCRNPDFNVYM
                     DFQPGDMQFINNYHVLHARTAFEDHPEPGRKRFLKRLWLETRKLTERPPYFQLGGSNK
                     SWWARQRTRAS"
     gene            complement(1884..3605)
                     /locus_tag="VF516_30500"
     CDS             complement(1884..3605)
                     /locus_tag="VF516_30500"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8112117.1"
                     /translation="MAHRPDLVLRNGTVIDGSGGAPFRADVAVAGGAITEVGAVGAAG
                     REEIDAAGCLVTPGFVDIHTHYDGQVTWDARLAPSSLHGVTTAVLGNCGVGFAPVQPD
                     HHQMLIELMEGVEDIPGAALHEGLPWTWSSFPEYLDAIERMPHDIDVCTQVPHGALRV
                     YVMGERGANREAASDEEIAAMADVVAQALRAGALGFTSSRTVQHRTSKGAPTPTLTAA
                     EAELRGIAAGLRRAGAGVIEMISDFDDLDGEFARFCRMVEDAGRPMTISLAQHDGAPG
                     RWKELLARIEGAQARGLPIKGQVAPRPIGVLLGLDCTLHPFVACPSYRAIARQPLAER
                     VRALRDPEMRSRLLGETAQSSNATVRYIAKSYHKLFRLGDPPDYEPDPSQSVAAQAER
                     AGRPPAELAYEWLLDNDGREILYFPLHNFSEQSLDAVRAMMAHDATLAGLGDGGAHVG
                     TICDASFTTYALTHWGRDRARGRFELPWLIKRQTHDTAAAVGLADRGLVRPGMKADLN
                     VIDFDRLRLRRPYIVHDLPANGRRLMQTAEGYVATVVSGAVTYRGGEPTGALPGKLVR
                     GAQPRRN"
     gene            4240..5325
                     /locus_tag="VF516_30505"
     CDS             4240..5325
                     /locus_tag="VF516_30505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112118.1"
                     /translation="MPFVQLKPRPDRSAPGSPPPGAQRRPGTGNQPQNHHQQDNPQQL
                     IAQLQQLIHDSDWDNQLRPQIYARAAQPIRERADARRQAQQQNNHPQQNDPQQPAVPD
                     LTGLGSVLRIDAFARGIKQIQQDWGTLGAPHARAQALCNACNTALRDIGVYPISDVEA
                     HPMEPRGAFNRKQWKLIFREQTLSQPAPLGGREAAGIADTALHETRHCEQHFRVARYL
                     AGQRQSAKTIAAQREIPIAVAQAAAEHPLTCQNGTSQEIQEAKQWKQQFGAKDQVQHD
                     HHIAGETGQEIEHLAQRRAAAQQALTDLQDHQTPHDLQRARLAARALAAQIAAVRKVY
                     AAYRAIPWEADAHEVGVSSARAFEWLP"
     gene            5322..5924
                     /locus_tag="VF516_30510"
     CDS             5322..5924
                     /locus_tag="VF516_30510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112119.1"
                     /translation="MSRIPLPPLVLMVAAAACTGRSPAQPTPPAGAERTPAQPTPRGD
                     AERAPAQPTPRGDEDPITEIRELWDLLSTDPLRADAVVRRIGSIIKDSGRELYIAPSN
                     RRFQAASVYREPKNGDVDIVDLTPAATQRPTVGALRAAFGPYEDASSLHFDVPATLRF
                     NTYHPGGPCTVTLFADLAEVGFDPPPTAAIGSFTFQRVCL"
BASE COUNT          925 a         2450 c         2157 g          883 t
ORIGIN      
        1 cccggtgcgc ggcggcggct tgatcagcta tgccgggagc ctgtgatgcg cgcgctcgtg
       61 gccttgttcg tgttcgtcgc cgtgctgacc gcgggctgcc tgcgcaccac cgagttccgg
      121 tgcgcgagca acgcggagtg cggcgccggc ggcgtgtgcg agctggtcgg gttctgcagc
      181 gttcctaatc cggaatgcgc aggaaccggc cgcagctaca gcgactccgc cggccagggc
      241 ctgtcgaaca cctgcgtgcc cgccggtgcg ccggggccgg gcaacgacgc cggcgtcgat
      301 gcaccggagg acgccatgcc gatccccaag tgtccctcgg gctatgccgc cgtcgccggc
      361 tccgcccatc tctacaaggc cctgtccggc gtcacgtggg acgaggccaa gatgatgtgc
      421 gatctgacct ccagggcggc gtacctcgcg gttcccgacg acgccaccga gctggccaac
      481 ctggccatcg tcgcggccgc gccgctctgg gtcggcatcg acgacaaggc gacctcgggc
      541 atgttcgtga cccagaaggg cgcgccggcg agcttcttgc cgtggggccc cggccagccc
      601 aacctcggcc cgcagaacga ctgcgtcacc gcgatctcga cgacgcagat cgcgaccgag
      661 aagtgcagca ccaagcacgc cgcggtctgc gagtgcgagc cgtagcgcag cgcgccggtt
      721 cttgacgagc tgccgatctc cgggttgccg agcggcgacc gggtcgtgcg tcgtggcaca
      781 ccgcgcgtgg gcgcacgcga tggccgcggg ggatcgacaa cccgctgccg gtcggcccgc
      841 gcgtcaggac gccctggtcc gctggcgcgc ccaccacgac ttgttcgagc ccccgagctg
      901 gaagtacggc gggcgctcgg tcagcttgcg ggtctccagc cacagccgct tgaggaaccg
      961 cttgcggccg ggctcggggt ggtcctcgaa cgccgtccgc gcgtgcagca cgtgatagtt
     1021 attgatgaac tgcatatctc cgggctggaa atccatgtag acgttgaagt ccggattgcg
     1081 gcacaaccga tccagcatgt cgaacgcctc gcgctcggcg gcggtgaccg ccggcacgcc
     1141 ggggtggcgc cgcgccgact cgatgtacgg ccggatgtag cgcacgaaca gccggtcgcc
     1201 gaggcgcgtg aacaccggca tcgtgtaggt cggcgagcgg ccgggctgct gctcgccgcg
     1261 gaagtcgtag atcatcggct ggtacagcac cgccgccagg tcgggccgcg tccgcaccat
     1321 ctcgttgtgc gcgagcaccg cgttggccac cgtgctgagg ccgcccgacc tggccttgcg
     1381 caggcacatc aggccgacca ggtccgagcc gtcggagtgg tacggcatgg cgaccgggcc
     1441 gagctggttg ccgcgcgacg tcgggtccac gtgggagcgc ccctgatcgg tcacgtcgcc
     1501 gagcaggtgg ccgtgctggt tctgcggcca cggcgtgccg aggtgcatgc cgatgcccca
     1561 gtagatcgcg ctcgcgtcgg cgtcgctgta gcgctcggcc ggcagcccgc tgatcagctg
     1621 gaagccgcgg ccgttgatca gctcgtcctc gaaccccgcg agcttcgccg ccagcgtcgg
     1681 cagcgggaag tcctcgcgcg tgatgtcgag cacgtcggcg cagcgcgccc tggcgtgggc
     1741 gagcgccgcc tcgagctcgg cgacctcggc gtcgctcagc cggtacgtcc agcgcgagcc
     1801 gtcggcgacg tcgcgcgcgc gccactccgc gtggatctcc agcggctcga gcgccggcgg
     1861 cggagcgatg tgggtggcca tggtcagttc ctcctcggtt gtgcgccgcg caccagcttg
     1921 ccgggcagcg cgccggtcgg ctcgccgccg cgatacgtca ccgcgccgct caccacggtc
     1981 gcgacgtagc cctcggccgt ctgcatcagc cgccggccgt tcgccggcag gtcgtggacg
     2041 atgtacggcc ggcgcagccg cagccggtcg aagtcgatca cgttgaggtc cgccttcatc
     2101 cccggccgca ccagcccgcg atccgcgagg ccgaccgccg ccgcggtgtc gtgggtctgg
     2161 cgcttgatca gccacggcag ctcgaaccgc ccgcgcgcgc ggtcgcggcc ccagtgggtc
     2221 agcgcgtacg tcgtgaagct cgcgtcgcag atcgtcccga cgtgcgcccc accgtcgccc
     2281 agcccggcca gcgtcgcgtc gtgcgccatc atcgcgcgca ccgcgtcgag cgactgctcg
     2341 ctgaagttgt gcagcgggaa gtacaggatc tcgcgcccgt cgttgtccag cagccactcg
     2401 tacgccagct ccgccggcgg acgccccgcg cgctcggcct gcgccgcgac gctctggctc
     2461 ggatccggct cgtagtccgg cggatcgccg agccggaaca gcttgtggta gctcttcgcg
     2521 atgtagcgca ccgtcgcgtt cgacgactgc gcggtctcgc cgagcagccg gctccgcatc
     2581 tccgggtcgc gcagcgcgcg cacccgctcg gccagcggct gcctcgcgat cgcccggtag
     2641 ctcgggcacg cgacgaacgg atgcagcgtg cagtccagcc cgagcagcac cccgatcggc
     2701 cgcggcgcga cctgcccctt gatcggcagc ccgcgcgcct gcgcgccctc gatccgcgcc
     2761 agcagctcct tccaccgccc cggcgcgccg tcgtgctgcg ccagcgagat cgtcatcggc
     2821 cgccccgcgt cctcgaccat ccggcagaac cgcgcgaact cgccgtcgag gtcgtcgaag
     2881 tccgagatca tctcgatcac ccccgccccc gcgcgccgca gccccgccgc gatcccgcgc
     2941 agctccgcct ccgccgccgt cagcgtcggc gtcggcgctc ccttgctcgt ccggtgctgc
     3001 accgtcctcg acgacgtgaa cccgagcgcc ccggcgcgca gcgcctgcgc caccacatcg
     3061 gccatcgccg cgatctcctc gtcgctcgcg gcctcgcggt tggccccgcg ctcgcccatc
     3121 acgtagaccc gcagcgcccc gtgcggcacc tgggtgcaca cgtcgatgtc gtgcggcatc
     3181 cgctcgatcg cgtcgaggta ctcggggaag ctcgaccacg tccacggcag cccctcgtgc
     3241 agcgccgcgc cggggatgtc ctcgaccccc tccatcagct cgatcagcat ctggtggtgg
     3301 tccggctgca ccggggcgaa cccgacgccg cagttgccga gcaccgccgt cgtcaccccg
     3361 tgcagcgacg acggcgcgag ccgcgcgtcc cacgtcacct ggccgtcgta gtgggtgtgg
     3421 atgtcgacga acccgggcgt cacgaggcag cccgccgcgt cgatctcctc gcggcccgcg
     3481 gcgccgaccg cgccgacctc ggtgatcgcg ccgccggcga ccgccacgtc ggcgcgaaac
     3541 ggcgcgccgc cgctcccatc gatcaccgtg ccgttacgga ggacgagatc tggtctgtgc
     3601 gccatggcga aactcccgct gaggagcgcg catcgtcgcg cagtgcctgg caaagtcaag
     3661 cagttaccga ccgcccggca agccggtgca ctcccgatga cgaccgcctc cgtctctccg
     3721 gcgccgtcct catcggtgac gagcttgcgc ggcgacaggg ccgcctgctc gacaggcgac
     3781 tcgaggaggc cgagcttcgc gacgccggcc gcacgctcga cagcatcgca tccttgcagc
     3841 tcaccacagc ttcacgaact ccgcgtgagc gcccacgcgc gttgccgcat gacccacttc
     3901 gcggcgtcga cagccctgtt cgcgcgccac atggtgtcgc gccggcggtg attctggccc
     3961 tggagactgc gaatgacgtt tgatcgacgt acggtgggtc gtggcgaccc ggcagagcgc
     4021 gcgtcgagcg cgccgggtgt gcccgggaag cgaacgctgt ccgagtcgct ggtcgccccg
     4081 gcggagagcg cacaggatcg acccgccgct ccatcgcccg caacccgagc agtcgcgtcg
     4141 agcccgggtc cgcccggcgc cgcgtcgccc gggcattcca tcgcggcgct gtttggtcgg
     4201 gtgcccgaga gtccgccggt cccgcatggc gcacccgcgg tgcccttcgt ccagctcaag
     4261 cccaggccgg accgctccgc gcccgggtcg ccgccgcccg gcgcgcagcg ccgccccggc
     4321 accggcaacc agccgcagaa ccatcaccag caggacaatc cgcagcagct gattgcacag
     4381 ctgcagcagc tcatccacga ctccgactgg gacaaccagc tacgccccca gatatacgca
     4441 cgggctgcac agccgatccg agagcgcgcc gatgcgcgcc gccaggcaca acagcagaac
     4501 aaccatccgc agcagaacga cccgcagcag ccagcggtgc ccgatctgac cggtctgggg
     4561 tcggtgctcc ggatcgatgc gttcgcgcgg gggatcaagc agatccagca ggactggggc
     4621 acgctgggcg ccccccatgc ccgggcacag gcgctgtgca acgcgtgcaa caccgcgctg
     4681 cgcgacatcg gcgtctaccc gatatcggac gtcgaggccc acccgatgga accgcgcggc
     4741 gcgttcaacc gaaaacaatg gaagttgatc ttccgcgagc agaccctgtc gcagcccgcg
     4801 ccactcggcg gcagagaggc ggcgggaatc gccgacaccg cgttgcacga gacgcggcac
     4861 tgcgagcaac attttcgcgt cgcgcgctat ctggccggcc agcggcagtc ggcgaagacc
     4921 atcgcggcgc agcgcgagat cccgatcgcc gtggcgcagg ccgcggcaga gcacccgctc
     4981 acctgccaga acggaacctc acaggagatc caggaagcga agcaatggaa gcaacagttc
     5041 ggtgccaaag accaagtcca gcacgaccat cacatcgccg gcgagacggg gcaggaaatc
     5101 gagcatctcg cccagcggcg cgccgcggca cagcaggcgc tgaccgacct gcaggaccac
     5161 cagacgccgc acgatctcca gcgagcccgc ctcgccgccc gcgcgctggc cgcgcagatc
     5221 gccgcggtcc ggaaggtcta cgccgcgtac cgggcgatcc cgtgggaggc cgacgcgcac
     5281 gaggtcggcg tgtcctcggc acgcgccttc gagtggctgc catgagccgc atcccgctgc
     5341 caccgctcgt cctgatggtg gctgccgcgg catgcaccgg gcgatccccg gcccagccca
     5401 cgcctcccgc aggcgccgag cgaaccccgg cccagccgac gccccgcgga gacgccgagc
     5461 gagccccggc ccagcccacg ccccgcggag acgaagaccc gatcaccgag atccgcgagc
     5521 tctgggacct gctgtccacg gatccactgc gcgccgacgc cgtcgtccgt cgcatcggct
     5581 cgatcatcaa ggactccggg cgtgagctgt acatcgcgcc gtcgaaccgg cgattccaag
     5641 cggccagcgt gtatcgcgag cccaagaacg gcgacgtcga catcgtggac ctcacccccg
     5701 ccgcgacgca gcgaccgacg gtgggcgcgc tgcgcgcggc gttcggcccc tatgaggacg
     5761 cgagctcgct ccacttcgac gtgcccgcca ctctccggtt caacacctac caccccggcg
     5821 gaccgtgcac agtcaccctc ttcgccgatc tcgcagaggt gggcttcgac ccgcccccca
     5881 cggccgcgat cggctcgttc acattccagc gcgtttgcct atgaccggcg cacacgccat
     5941 cgcggatctg ctcgacctcc tcgaacgcgt cccgggccgc gcccctgtca tcccgggtga
     6001 aagcccgtcg gtcgtggtgt ccctgcgggt cggcgcctcg gtgcaacaag ccggagccac
     6061 ccccatgact gttctcctga ggttgtccaa cgacgaccgg tcacgcgcga ggaacccgca
     6121 ataaagagag cgtcatgacg gcaaggccac accgccgtca cgacgccttg ctggaccgcg
     6181 tacgctcgca atcgctcggg cagctctccg cgcgtcctcg tcgcggctcg ctcatgacgg
     6241 gggacctcac ttccctccta gctggatctc ccggatccct tcggtcggcg gctcggcatg
     6301 gcgcctcggc ggcgcgggga tgcgcgacgc gggtgcgcgg tcaggcggcg cggcggcgtc
     6361 ggaggcggtc gtggtgtccg gcctcgcgcc cgcggggtga cccggcgacg gcacc
//
LOCUS       DASVOR010001628         1843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_74967,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001628 DASVOR010000000
VERSION     DASVOR010001628.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1843
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74967"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VF516_30515"
     CDS             <1..252
                     /locus_tag="VF516_30515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112120.1"
                     /translation="ADNAQEVLAAALCVYPRSRPLRSLYYVASAISALGKGEVMLATS
                     QLETALAHHEDCIEAAAILELLRRPGTPVASHEMTRLFR"
     gene            249..1811
                     /locus_tag="VF516_30520"
     CDS             249..1811
                     /locus_tag="VF516_30520"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8112121.1"
                     /translation="MTRAIGIDLGTSNSVCAVIDQDGVPRILTTPEGATTMPSLVWFS
                     PDGPVVGELARAGLDYSPDLTIFGAKRLLGRRFDHPEIRRLARVLPFELIEAPNGDTW
                     IALSAGRAVSPEEVCALILREIRRMAEDHFGEPVTRAVITIPAWYDAAQRQATKDAAQ
                     IAGLTVLRLLSEPTAASLGHGAHRGADRRYLVCDLGGGTFDVAAVDVEAGIFEVLATT
                     GDAFLGGDDVDRIVVENLVRDVRSARGFDITSDTVAVERLRLAAQTTKHELSDVTASK
                     IYVPELLQLPSGRAVEYQKPLRRDELEQWSSPLIHRLEAPIREALRRCGRKREDIEEV
                     LLVGGMTRMPAVRREIARVTGREPHVIPNPEEVVAVGAALEVARLDGTIEGVLLIDVA
                     ARGLAVSMGGDCEQVIAQSSVVPTREHRVFGTRHDDQLRIEFDVWEGESPDPADNRHL
                     GRYAVLDLPPAPAGDVLVLVEMTIDTDGTVRLGASELVSGERLQLEQLYHAGLARTEV
                     LRLQRQFEEAGR"
BASE COUNT          248 a          676 c          676 g          243 t
ORIGIN      
        1 gccgacaacg cccaggaggt gctcgccgcg gcgctgtgcg tctacccgcg cagccggccg
       61 ctgcgctcgc tgtactacgt cgcgtcggcg atcagcgcgc tcggcaaggg cgaggtcatg
      121 ctcgcgacct cgcagctgga gaccgcgctc gcccaccacg aggactgcat cgaggccgcc
      181 gcgatcctcg agctgttgcg ccgccccgga acgccggtcg cgagccacga gatgacgagg
      241 ctatttcggt gacccgggcg atcggcatcg atctcggcac ctcgaactcg gtgtgcgcgg
      301 tgatcgacca ggacggcgtg ccgcgcatcc tcaccacccc cgagggtgcg accacgatgc
      361 cgtcgctggt gtggttctcg cccgacgggc cggtggtcgg cgagctcgcc cgcgccggcc
      421 tggactacag ccccgatctg acgatcttcg gcgccaagcg gctgctcggc cggcggttcg
      481 atcaccccga gatccgccgg ctcgcgcggg tcctgccgtt cgagctgatc gaggcgccca
      541 acggcgacac ctggatcgcg ctgtcggccg gccgcgcggt gtcgcccgag gaggtctgcg
      601 cgctgatcct gcgcgagatc cggcggatgg ccgaggacca cttcggcgag ccggtgaccc
      661 gcgcggtgat cacgatcccg gcgtggtacg acgccgccca gcgccaggcg accaaggacg
      721 cggcgcagat cgccgggctc accgtgctgc ggctgctcag cgagcccacc gcggcgtcgc
      781 tcggccacgg cgctcaccgc ggcgcggatc gccgctacct ggtgtgcgac ctcggcggcg
      841 gcacgttcga cgtcgcggcg gtcgacgtcg aggccggcat cttcgaggtc ctggcgacca
      901 ccggcgacgc gttcctcggc ggcgacgacg tcgaccgcat cgtggtcgag aacctggtcc
      961 gcgacgtgcg ctcggcgcgc ggcttcgaca tcaccagcga caccgtcgcg gtcgagcggc
     1021 tgcggctcgc cgcgcagacc accaagcacg agctgtccga cgtcacggcc agcaagatct
     1081 acgtccccga gctgctccag ctgccgtcgg gccgcgccgt cgagtaccag aagccgctgc
     1141 gccgcgacga gctcgagcag tggtcgagcc cgctgatcca ccggctcgag gcgccgatcc
     1201 gcgaggcgct caggcgctgc ggccgcaagc gcgaggacat cgaggaggtg ctgctggtcg
     1261 gcgggatgac ccggatgccg gcggtgcgcc gcgagatcgc gcgggtcacc ggccgcgagc
     1321 cccacgtgat ccccaacccc gaggaggtcg tcgcggtcgg cgccgcgctc gaggtcgcgc
     1381 gcctcgacgg cacgatcgag ggcgtgctgc tgatcgacgt cgcggcgcgc gggctcgcgg
     1441 tgtcgatggg cggcgactgc gagcaggtga tcgcgcagag ctcggtcgtg ccgacccgcg
     1501 agcaccgggt gttcgggacc cggcacgacg accagctgcg catcgagttc gacgtctggg
     1561 agggcgagtc gccggatccg gcggacaacc gccacctcgg ccgctacgcc gtgctcgatc
     1621 tgccgccagc gccggcggga gacgtgctag tcctcgtcga gatgacgatc gacaccgatg
     1681 gcacggtccg gctcggcgcc agcgagctgg tgtccggcga gcggctccag ctcgagcagc
     1741 tctatcacgc gggcctcgcc cgcaccgagg tcctccggct ccagcgccag ttcgaggagg
     1801 cggggcgatg accgcggcgc ggacccgggc ggcgcggctc gcg
//
LOCUS       DASVOR010001629         3131 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_425142,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001629 DASVOR010000000
VERSION     DASVOR010001629.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425142"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VF516_30525"
     CDS             <1..1198
                     /locus_tag="VF516_30525"
                     /inference="COORDINATES: protein motif:HMM:NF020243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta-ketoacyl reductase"
                     /protein_id="HEX8112122.1"
                     /translation="GSLRGDAAYLITGGLGGIGLAVARWLVERGARHLVLTGRTADAA
                     TSAQRSCIAELERRGAAVRLAQVDASDEQGMAALLDDLRRGAQPLRGVFHAAGVSAPR
                     TIAELDASALAAVLAPKVAGAWILHRLTRDRELDHFVCFSSISSILGSRRLAGYAAGN
                     AFLDALAHHRVALGLPALSVNWGPWAEVGMTLPAERAAMERIGMHALPTREALAIVDR
                     VLGGDAPQIMAARIDWSAFAPALEARAPRPLLDAVRVRAADTPAEEPELRRRLAATDP
                     DARVALVVDFLRGEVARVLGRDTAAAIDVEQGFFEMGLDSLMAVELKRRLERATGLML
                     PRTIAFEFPNVDALARHLCTVCACPPPSTASTAATLPAPPALSEREAEALLLDELRSL
                     EQEMRV"
     gene            1195..>3131
                     /locus_tag="VF516_30530"
     CDS             1195..>3131
                     /locus_tag="VF516_30530"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I polyketide synthase"
                     /protein_id="HEX8112123.1"
                     /translation="MSTSEPGTHRDVLQRALSAVKDLRARLATAEHAPTEPIAIIGIG
                     CRFPGGVHDPDSFWRLLSDGVDAIGEVPADRWDAEAYYDADPQVPGKITTKQGGFLDQ
                     VDRFDAAFFRIAPREAVHLDPQHRLLLEVAWEALERAGQTRASLDGSSTGVFVGITTG
                     DYGEILRRAGVDGLDAYFPTGNAFNAAAGRLSYLLGLQGPSMAVDAACASSLVAIHLA
                     CQSLHTGDCRLALAGGVNLMLEPDAHIAMSRAHALAPDGRCKTFDASADGYGRGEGCG
                     IVVLKRLSDAIADRDPILAVVRGSAVMQDGHSGGLTVPNGAAQQAVIRAALAHAGVAP
                     GDVSYVEAHGTGTSLGDPIEVRALGAVFGPGRSPGHPLVLGSVKTNIGHLESAAGVAG
                     LIKVVLALQHRAIPPHLHLRELNPDVAQEPMPKLIPSATMPWPGDGPRIAGVSAFGLS
                     GTIAHMVVEEAPARPAAESEPDHALVLPISAHSPEALRELATAYHDLVSRRPDAPAVR
                     DLCYSAAVRRTHHDHRLAVVGETHAELAERLSAFLDGTPCSGLAAGIRGSAAHKLVFV
                     FPGQGGQWSGMAQDLLAREPVFADAIAACEAALRPHVDWWLRELLAASPAPPLLERID
                     VIQPALFSLQVALAALWRSLG"
BASE COUNT          390 a         1131 c         1143 g          467 t
ORIGIN      
        1 cgggtcgctg cgcggcgacg ccgcgtatct gatcaccggc ggtctgggcg ggatcggact
       61 ggcggtggcg cgctggctgg tcgagcgcgg cgcacgccac ctggtgctga ccgggcgcac
      121 ggccgatgct gcgaccagcg cacagcgcag ctgcatcgcc gagctggagc gccgtggagc
      181 cgccgtccgc ctcgcgcagg tcgatgccag cgacgagcag ggcatggccg cgctgctcga
      241 cgaccttcgc cgcggcgcgc agccactgcg cggcgtgttc cacgcggccg gggtctccgc
      301 gccgcgaacc atcgccgagc tcgacgcgag cgcgctcgcc gctgtgctcg caccgaaggt
      361 cgcgggtgcg tggatcctgc accggctgac gcgcgatcgg gagctcgacc acttcgtctg
      421 cttctcgtcg atctcctcga tcctcggctc gcgccggctc gccgggtacg cggcgggcaa
      481 cgcgttcctg gatgcgctcg cgcaccaccg cgtcgcgctc gggctgccgg cgctgagcgt
      541 gaactgggga ccgtgggccg aggtcggcat gacgctgcct gccgagcgag ccgcgatgga
      601 gcggatcggc atgcacgcgc tgccgacgcg cgaggcgctc gcgatcgtcg atcgcgtcct
      661 gggaggcgat gcgccgcaga tcatggccgc ccgcatcgac tggagcgcgt tcgcgccagc
      721 gctggaagcc agagcgccgc gtccgctgct cgacgccgtg cgcgtccgcg ccgctgacac
      781 gcccgccgag gagccagagc tccgccgacg cctcgcagcg acggacccgg acgcgcgcgt
      841 ggcgctggtg gtggacttcc tgcgcggcga ggtcgcccgc gtgctcggcc gcgacaccgc
      901 cgccgcgatc gacgtcgagc agggcttctt cgagatgggc ctcgactcgc tcatggcggt
      961 cgagctgaag cgccggctgg agcgggccac ggggctgatg ttgccgcgca cgatcgcgtt
     1021 cgagttcccg aacgtcgatg cgctggcccg gcacctgtgc accgtgtgcg cctgtccgcc
     1081 cccatcgact gcctcgacgg cagccacctt gccggctcca ccggcgcttt cggagcgcga
     1141 ggcagaggcg ctcctcctcg atgagctccg gagcctggag caggagatgc gtgtatgagt
     1201 acatcggagc ccggaaccca ccgggacgtc ctccagcgag cgctgtccgc ggtgaaggat
     1261 ctgcgcgcgc ggctcgcgac ggccgagcat gctcctaccg agccgatcgc catcatcggg
     1321 atcggctgcc gcttccccgg cggggtgcac gacccggact cgttctggcg cctgctgtcc
     1381 gacggtgtcg acgcgatcgg cgaggttcct gcggatcgct gggacgccga ggcgtactac
     1441 gacgccgatc cgcaggtacc cggcaagatc accacgaaac agggcgggtt cctcgatcag
     1501 gtcgatcggt ttgacgcggc gttcttccgg atcgccccac gcgaggctgt gcacctcgat
     1561 ccgcagcacc ggctgctgct ggaggtcgcc tgggaggcgc tggagcgcgc cggtcagacc
     1621 cgcgcgtcgc tcgacgggag ctcgaccggc gtgttcgtcg gcatcaccac gggcgactac
     1681 ggcgagatcc tccgtcgcgc gggcgtcgac ggactcgatg cgtatttccc gacgggcaac
     1741 gcgttcaatg ccgcagccgg ccggctgtcg tacctgctcg gcctgcaggg tccgagcatg
     1801 gcggtggacg ctgcctgcgc gtcgtcgctg gtcgcgatcc atcttgcctg ccagagcctg
     1861 cacaccggcg attgccggct cgcgctcgcc ggcggcgtga acctcatgct ggagccggac
     1921 gcgcacatcg cgatgtcacg cgcccacgcg ctcgcgccgg acggtcggtg caagacgttc
     1981 gacgcgagcg cggacggcta cgggcgcggc gaaggctgcg gcatcgtggt gctgaagcgg
     2041 ctgtccgacg cgatcgccga ccgcgatccg atcctcgccg tggtgcgcgg ctcggcggtg
     2101 atgcaggacg gccacagcgg cgggctcacc gtgcccaacg gcgccgcgca gcaggcggtg
     2161 atccgcgcgg cgctcgcgca cgcgggtgtc gcgccgggtg acgtgagcta cgtggaggcg
     2221 cacgggaccg gtacgtcgct cggcgatccg atcgaggtgc gcgcgctcgg tgcggtgttc
     2281 ggtcccggcc gcagcccggg gcatccgctc gtgctgggat cggtgaagac caacatcggc
     2341 cacctggagt cggccgccgg tgtcgccggg ctgatcaagg tcgtcctcgc gctgcagcac
     2401 cgcgcgatcc cgccgcacct tcacctgcgc gagctcaatc ccgacgtcgc ccaggagccg
     2461 atgcccaagc tgatcccatc ggcgacgatg ccgtggcccg gcgacggtcc gcgcatcgcc
     2521 ggtgtcagcg cgttcggcct gagcgggacg atcgcgcaca tggtggtcga ggaggcgccg
     2581 gcgaggcctg ccgccgagag cgagcccgac cacgccctcg tgctgccgat ctcggcgcac
     2641 agccctgagg cgctgcgcga gctcgctacc gcgtaccacg acctggtgtc tcgtcgcccg
     2701 gacgctccag ccgtccgcga tctgtgctac tcggccgcgg tccgtcgtac ccatcacgat
     2761 caccggctcg cggtggtcgg cgagacccac gccgagctcg ccgagcggct gtccgcgttc
     2821 ctcgacggca cgccgtgcag cgggttggcc gccgggatcc gcgggtccgc cgcgcacaag
     2881 ctggtgttcg tgttcccggg gcagggcggc cagtggtcgg gcatggcgca ggatctgctc
     2941 gcccgcgagc cggtgtttgc cgacgcgatc gcggcgtgcg aggccgcgct ccggccccac
     3001 gtcgactggt ggctgcgcga gctgctcgct gcctcgcccg caccgccgct gctggagcgg
     3061 atcgacgtca ttcaaccggc gctgttctcg cttcaggtcg cactggcggc gctgtggcga
     3121 tcgctcggta t
//
LOCUS       DASVOR010001630         3219 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_513543,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001630 DASVOR010000000
VERSION     DASVOR010001630.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3219
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_513543"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..466)
                     /locus_tag="VF516_30535"
     CDS             complement(<1..466)
                     /locus_tag="VF516_30535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112124.1"
                     /translation="MTRTPTSSTKRIAPAQAAKLSKAPKKSSKPVKKRNTEVRKVKEA
                     QMIRSKIAVPIEIARTAIAYDPTLGGNVGFLKIAGGTPPLKPPNGDTLLHLLFFLIYT
                     EDGWDLLRANGPTSAATPDQARANLKAALLRLFPTIEDNRPDSVLDPVIDAHF"
     gene            complement(554..1309)
                     /locus_tag="VF516_30540"
     CDS             complement(554..1309)
                     /locus_tag="VF516_30540"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8112125.1"
                     /translation="MLERANKLRAVLVENEEHSLSRLRRLLQGFPHDVEVVGEASDGP
                     SAVDTIRAQVPDIVFLDIDLPGFNGFQVLSRLDRQPIVIFTTAYNEHALDAFKTHAID
                     YLLKPLEAEAIGGALAKLRAMGFDQGHLARAMEQFLRPPAYLSRLQCKESERTVVLKI
                     GEILYLQSDNKYTSVHASNRECLIDTPLIELERKLDPSEFIRIHRSTLVNVSWIAEIQ
                     RHPYESKTTVVLRDAKRTELTVSRMYADNLRKI"
     gene            1715..1912
                     /locus_tag="VF516_30545"
     CDS             1715..1912
                     /locus_tag="VF516_30545"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8112126.1"
                     /translation="MSQTSPETARTGVAHERRGREKARGQRPERTLEEVAATLNVTRE
                     RVRQLEARGLTALRRARRRLT"
     gene            complement(1916..2185)
                     /locus_tag="VF516_30550"
     CDS             complement(1916..2185)
                     /locus_tag="VF516_30550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112127.1"
                     /translation="MTSESTEDVINTCNPVNYECDPNDPFSDRLCQIVCDPDGTQGYC
                     LEYSSIESAWCAAHPDKFYRAIPFMYCDINGNPTWDTHCEPGWLP"
     gene            complement(2233..2487)
                     /locus_tag="VF516_30555"
     CDS             complement(2233..2487)
                     /locus_tag="VF516_30555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112128.1"
                     /translation="MGNVKRMLTAIKVALPGLTAREWGAIAIEASRKAGLCEPDVSSL
                     RTNLQNEWAIATAEHGDRHHDRTAPRTPRATAQRGTSGGC"
     gene            complement(2843..>3219)
                     /locus_tag="VF516_30560"
     CDS             complement(2843..>3219)
                     /locus_tag="VF516_30560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112129.1"
                     /translation="RNTGTPVSTGTGAPVGTGTGAPVGTGSSSVPVPPPLPAGRQCLP
                     AMICNDWAGCALVENSKVVSADRLVAGEPVSIGNGCTTGATCIAARAVPKGVTCPVDT
                     IPPRIDPPLYTCAWDGTACVKR"
BASE COUNT          466 a         1076 c         1149 g          528 t
ORIGIN      
        1 cgaagtgcgc atcgatgacc gggtcgagca ccgagtcggg tcgattgtcc tcgatcgtcg
       61 ggaacagtcg caggagtgcc gccttgaggt tcgccctggc ctgatccggc gtggcggcgc
      121 tggtcggccc gttcgccctc agcagatccc acccgtcctc ggtgtagatc aggaagaaca
      181 gcagatggag cagcgtgtcg ccgttgggcg gcttgagcgg cggcgtgccg cccgcgatct
      241 tcaggaagcc cacgttcccg ccgagcgtgg gatcgtaggc gatcgccgtc cgggcgatct
      301 cgatcggcac cgcgatcttc gatctgatca tctgcgcctc cttcaccttc cgcacctcag
      361 tattccgctt cttcaccggc ttgctcgact tcttcggcgc cttgctgagc ttggcggcct
      421 gagccggtgc gatccgcttg gtcgacgacg tgggggttcg tgtcatggga tggctccgtt
      481 gtggtctggg cattccggca tctggcgttc cagcgcgctg attcacgaca cgaaaaacac
      541 cgggccgccg cgatcagatc tttcggaggt tatccgcata catccgactg accgtgagct
      601 cggtgcgctt ggcgtctcgg agcacgacgg tggtcttgct ctcgtacgga tggcgctgga
      661 tctcggcgat ccaggacacg ttgacgaggg tcgagcggtg aatacggatg aactccgagg
      721 gatcgagctt gcgctcgagc tcgatcagcg gtgtgtcgat caggcattcg cgattcgatg
      781 cgtgaaccga cgtgtacttg ttatcggact ggagatagag gatctcgccg atcttgagca
      841 cgaccgtgcg ctcgctctcc ttgcattgca ggcgcgacag gtacgcgggg ggccgcagga
      901 attgctccat cgcgcgcgcg aggtggccct ggtcgaagcc catggcgcgc agcttggcca
      961 gcgcgccgcc gatcgcctcg gcctcgagcg gcttgagcag gtagtcgatc gcgtgggtct
     1021 tgaacgcgtc cagggcatgc tcgttgtacg ccgtcgtgaa gatcacgatg ggctggcggt
     1081 cgagcctcga cagcacctgg aacccgttga agccgggcag gtcgatatcc aggaacacga
     1141 tgtcaggcac ctgcgcgcgg atcgtgtcga ccgcggacgg gccatcgctg gcctcgccga
     1201 cgacctcgac atcgtgcggg aacccctgca gcaggcgccg gagccgcgac agcgagtgct
     1261 cctcgttctc gaccaggacg gcgcgcagct tgtttgcccg ttccagcatc gtgcgccgga
     1321 tccccccggc cggcccaaca tatcaccggg gtcgcagcca ccgggggcga tccgatgagt
     1381 gacctggcgg gccgatgatc cgtcgtccgc ggcggacgag acgcaggccg cgccccacgt
     1441 cccccgatcg ccgtgccgcc cggcgcggca gccacccgcc ggcgcgcatg cgggtcggtc
     1501 cgtgctggct caggcaacga ttccagcgag gctggaacat gatgcgggag tgtcgtgaac
     1561 acgtggcttc cggcgcctat gtccacgacg tggccggacg gccccggagg gcagcgtggg
     1621 tccgctgaac caccaccacg acgtcggccc gcccttgcgg cccgccggag tgggatgtcg
     1681 gcatctcgta cgttggcgac tccaatcagc tatcatgagc caaaccagcc cagaaacagc
     1741 gcgaacagga gtcgcacatg agcggcgggg tcgagagaag gccagggggc agcggcccga
     1801 gcggacgctc gaggaggtcg cggcgaccct caacgtcacc cgcgagcgcg tgcgccagct
     1861 cgaggcccgc gggctcacgg cgctgcgccg cgcgcgccgc cggctgacct gagcgctacg
     1921 gcagccagcc cggctcgcag tgcgtgtccc acgtcgggtt gccgttgatg tcgcagtaca
     1981 tgaacgggat cgcgcggtag aacttgtccg ggtgggcggc gcaccacgcg ctctcgatgc
     2041 tgctgtactc gaggcagtac ccctgcgtgc cgtcggggtc gcagacgatc tggcacaggc
     2101 gatccgagaa cggatcgttg gggtcgcact cgtagttgac cgggttgcag gtgttgatga
     2161 cgtcctcggt gctttcggag gtcatcggtg cggcgcaggc agcgagcgcc agggcaacgg
     2221 cggcggtgcg gatcagcatc cgcccgaggt accgcgctgc gcggtcgcgc gcggcgtgcg
     2281 tggggcggtc cgatcgtgat ggcggtcccc gtgctccgct gtcgcgatgg cccactcgtt
     2341 ctgcaggttg gtccgcaggc tcgatacgtc cggttcgcac agcccggcct tgcgcgaggc
     2401 ctcgatcgcg atcgctcccc actcgcgcgc cgtgagcccg ggcaacgcga ccttgatcgc
     2461 cgtgagcatc cgcttgacgt tccccaccag gatctccccg ggtcgatgtc ccgatccggc
     2521 tcggtctcgg cgcggcggtg gcggccgctg cccttcgcgg cgtcctcggg gcacaggtcg
     2581 ggcaccggcg gcgccgcgcc agccaccgcg aagagcgcat catcggcgct cgcgacggcc
     2641 gcgcggatgc gggcggcgag gccgcgggcg cgctccgcgg acatcgagac cgcgacgatc
     2701 cgcgagatcg ttcgcggcga gctcggcagc agcctcaccc cgtcacgtac cgaaagagtc
     2761 cgcgatccac ttctggcaca cgacgggaat gcgatggata gccagcatgg ctcgaccgcg
     2821 gcgctgcgcc cgggggatgc gctcagcgct tgacgcaggc ggtgccgtcc caggcgcagg
     2881 tatagagggg cggatcgatg cgcggcggga tggtatccac cgggcaggtg acccccttgg
     2941 gcaccgcgcg ggcggcgatg caggtcgcgc cggtcgtgca gccgttgccg atgctgaccg
     3001 gctccccggc gaccaggcga tcggcggaca ccaccttgct gttctcgacg agggcgcagc
     3061 cagcccagtc gttgcagatc atggccggca ggcactgccg cccggcgggg agcggaggtg
     3121 gtaccggtac gctgctgctg ccggtgccga ccggggcgcc cgtgccggtc ccgaccgggg
     3181 cgcccgtgcc ggtgctgacc ggcgtgccgg tgttccggc
//
LOCUS       DASVOR010001631         3173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_17830,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001631 DASVOR010000000
VERSION     DASVOR010001631.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17830"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1722)
                     /locus_tag="VF516_30565"
     CDS             complement(70..1722)
                     /locus_tag="VF516_30565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112130.1"
                     /translation="MTPRWSAITALAACAGPPELPAARFANAPPVLAVDDRLDVPTPP
                     APREVFMDVYHWDGVVQRRIDRGLALLRPRRALGVNALDEVPDSTWFTNRIGMRDMTI
                     DELVSGPLTLDSPEGHRPWIIQSAKPGNSDIGFIVRDARGLKFLIKFDPAGYPEQETA
                     THVIVDKILWACGYNTTEDFLVHLRRDDLVLAPDASFSDVFGDKHRLDAALVDQKLQL
                     VERMADGRYRALASRWIDGKTLGGHPAEGTRDDDRNDRIPHELRRDLRGAHAFFAWLD
                     HVDVQESNFLDAWIADRDNPRIHYVKHYLIDFGKSFGVMASTGHDPRRGHAYVIDLAA
                     LARSLVQFGLVERSWEGRSAPALRGVGLFEARTYDPGAWVPDYPVYVPLLLADRFDAF
                     WASKILLRFTRSQLRALVETGQLSDPRAVEYVTDTLVARQRATAAYWFARVSPLDRFA
                     VYRDRLCFDDLAVAYRFAAAATTRYDVRRYDRQAQPLGASDARGAPDGRLCVPLALPA
                     TGDGYTMVRIATTRPGFTGATVVHIARDPASDAPRVIGIWRE"
     gene            complement(1719..3068)
                     /locus_tag="VF516_30570"
     CDS             complement(1719..3068)
                     /locus_tag="VF516_30570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112131.1"
                     /translation="MLTPLPGRDLTAAEVATAPLPGSETGRIDPPDGDSTWRQIARGA
                     LYVPRLVVDALLFPFRGALWVNEHDRVADWYDRIFYNDAHTIALYPTAAIDTTLGVTI
                     GARFVDLDLFGEREQLGLQATFGSWYRQIYSVSLGSGRRLGERFSLALDAAYERRPHD
                     AFYGIGNGDLHHDVPVPPEPIIDPRVESTAIETFYRQDRARVTLLADLCAWRQLHLRA
                     AGAASKVDFGHPDQGAPITAFYEPSGLIGLGGFRYVYGELELRWDSRRNATSWEPVSV
                     HSLGTLAAVFAGRTHRLDGGPGYTRYGVDVAQFMRIAEGPRVLIVHFHGEGVTGRLDQ
                     VPFTELPALGGPSYLRGYALDQFRDRIAAFVSLAYQWDLSQSFAARLFTDVGRVFPSL
                     DELSLHHLRVGYGLAVEAHSASDFDLEASIGSSIDGGLFFNLTFNPVYDIQERVRRR"
BASE COUNT          456 a         1022 c         1265 g          430 t
ORIGIN      
        1 ggagttcttg aagacgcagt agtcggccga ggtcgtgagc tcgtcgcacg atcccaggta
       61 cccgacggtc tactcgcgcc agatgccgat cacgcggggc gcgtccgagg cggggtcgcg
      121 ggcgatgtgg acgacggtgg cgccggtgaa ccccggacgg gtggtggcga tccggaccat
      181 ggtgtagccg tcgccggtgg ccggcagcgc cagcgggacg cagagccggc cgtcgggtgc
      241 gccacgggcg tcgctcgcgc cgagcggctg cgcctggcgg tcgtagcgac gcacgtcgta
      301 gcgggtggtg gcggcggcgg cgaagcggta ggcgaccgcg aggtcgtcga agcacagccg
      361 gtcgcgatag acggcgaagc ggtcgagcgg gctgacccgg gcgaaccagt aggcggcggt
      421 ggcgcgctgg cgcgcgacca gggtgtcggt gacgtactcg acggcgcgcg ggtcggacag
      481 ctggccggtc tcgacgaggg cgcgcagctg gctgcgggtg aagcggagga ggatcttcga
      541 cgcccagaac gcgtcgaagc ggtcggcgag caggagcggg acgtagacgg ggtagtcggg
      601 gacccaggca ccggggtcgt aggtccgggc ctcgaacagg ccgacgccgc gcagcgccgg
      661 cgcgctgcgg ccttcccagg accgctcgac caggccgaac tggaccagcg agcgggcgag
      721 cgcggcgagg tcgatcacgt aggcgtggcc gcgccgcgga tcgtggccgg tgctcgccat
      781 cacgccgaag ctcttgccga agtcgatcag gtagtgcttg acgtagtgga tcctggggtt
      841 gtcgcgatcg gcgatccagg cgtcgaggaa gttgctctcc tggacgtcga cgtgatcgag
      901 ccaggcgaag aacgcgtggg ccccgcgcag gtcgcggcgc agctcgtgcg ggatgcgatc
      961 gttgcggtcg tcgtcgcgcg tgccctcggc ggggtggccg cccagggtct tgccgtcgat
     1021 ccagcgcgac gcgagggcgc ggtagcggcc gtcggccatg cgctcgacga gctggagctt
     1081 ctggtcgacg agcgcggcgt cgagccggtg cttgtcgccg aacacatcgc tgaagctggc
     1141 gtcgggggcg aggacgaggt cgtcgcggcg caggtgaacg aggaagtcct cggtggtgtt
     1201 gtagccgcat gcccacagga tcttgtcgac gatgacgtgg gtcgcggtct cctgctcggg
     1261 gtagccggcg ggatcgaact tgatcaggaa cttcaggccg cgggcgtccc ggacgatgaa
     1321 gccgatgtcg ctgttgccgg gcttggcgct ctggatgatc cacggcctgt ggccttcggg
     1381 gctgtcgagg gtgagcgggc cgctgaccag ctcgtcgatc gtcatgtcgc gcatgccgat
     1441 ccggttggtg aaccaggtcg agtcgggcac ctcgtcgagg gcgttgacgc cgagcgcgcg
     1501 gcgcggccgc agcagcgcca ggccgcggtc gatccggcgc tgcacgacgc cgtcccagtg
     1561 atagacgtcc atgaacacct cgcgcggggc cggcggggtc gggacgtcga gccggtcgtc
     1621 gaccgcgagg acgggcgggg cgttggcgaa ccgcgcggcg ggcagctccg gcggaccggc
     1681 acacgccgcg agcgcggtga tcgcggacca ccgcggcgtc atcgccgcct cacgcgctcc
     1741 tggatgtcgt agaccgggtt gaacgtcagg ttgaagaaca gcccgccgtc gatcgacgag
     1801 ccgatgctgg cctcgaggtc gaagtcgctg gcgctgtggg cctccacggc gaggccgtac
     1861 cccacgcgga ggtggtggag cgacagctcg tcgagcgacg ggaacacccg gccgacgtcg
     1921 gtgaacagcc gcgcggcgaa cgactgcgac aggtcccact ggtaggcgag cgagacgaac
     1981 gcggcgatcc ggtcgcggaa ctggtcgagc gcgtagccgc gcaggtagct cggtccgccg
     2041 agcgccggca gctcggtgaa cggcacctga tcgaggcggc cggtcacgcc ctcgccgtgg
     2101 aagtggacga tcagcacgcg cgggccctcg gcgatccgca tgaactgcgc gacgtcgacg
     2161 ccgtagcgcg tgtagccggg tccgccgtcg agccggtggg tgcggccggc gaacaccgcg
     2221 gccagcgtgc ccaggctgtg gaccgacacc ggctcccagc tcgtcgcgtt gcgccggctg
     2281 tcccagcgca gctcgagctc gccgtagacg taccggaagc cgcccagccc gatcagcccg
     2341 ctcggctcgt aaaacgcggt gatcggcgcg ccctgatcgg gatggccgaa gtcgaccttg
     2401 gaggccgcgc cggcggcgcg caggtggagc tgccgccacg cgcacaggtc ggcgagcagc
     2461 gtgacgcgcg cccggtcctg gcggtagaac gtctcgatcg cggtcgactc gacccgcgga
     2521 tcgatgatcg gctccggcgg cacggggacg tcgtggtgca gatcgccatt gccgatcccg
     2581 tagaatgcgt cgtgcgggcg gcgctcgtag gcggcgtcga gcgccagcga gaatcgctcg
     2641 ccgagccggc ggccgctgcc gaggctcacc gagtagatct ggcggtacca gctgccgaac
     2701 gtggcctgca gtccgagctg ctcgcgctcg ccgaacaggt cgaggtcgac gaaccgcgcg
     2761 ccgatcgtga cgccgagggt ggtgtcgatc gccgcggtgg gatacagcgc gatcgtgtgc
     2821 gcgtcgttgt agaagatccg atcgtaccag tcggcgacgc ggtcgtgctc gttgacccag
     2881 agcgcgccgc ggaacggaaa cagcagcgcg tcgaccacca ggcgaggcac gtacagcgcg
     2941 ccgcgggcga tctggcgcca cgtggagtcg ccgtccggcg gatcgatgcg cccggtctcg
     3001 gatccgggca gcggagcggt ggcgacctcg gccgcggtga gatcgcggcc cggcaggggc
     3061 gtcagcaccg gagtttgaac cggggtctgc accgggcgct cgccgggtgg gcgcggctcg
     3121 ccgacgccga cggcgcgagc cggttgcgcg ggctccgggt ccgccgcggc ggc
//
LOCUS       DASVOR010001632         8910 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_221459,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001632 DASVOR010000000
VERSION     DASVOR010001632.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8910
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221459"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VF516_30575"
     CDS             complement(<1..795)
                     /locus_tag="VF516_30575"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8112132.1"
                     /translation="MTEIRHPHVFLISDLHLGDPAGEVDHFHSDEQLCAFLSSYLPDR
                     TNGEAATLIILGDFIDFPRIPPRYKELEHPTIGSTEDESVRKVMRAIKGHPLVFQGLR
                     ALIERGDQLLLLAGNHDVDLYWPGVRSQLASALRCEEPQLGFVHKGVLRDRGIHVQHG
                     HQLSFENQFQQWPPEPFRAFGGEQRLERCWGTFFMEAVYNLVEAFAPWIDLVHPTWRA
                     VLLALKYRGWDGIPPPVAAAFLAFFARFGKRVLADHLLSEDAGQGSR"
     gene            complement(792..2084)
                     /locus_tag="VF516_30580"
     CDS             complement(792..2084)
                     /locus_tag="VF516_30580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HEX8112133.1"
                     /translation="MNGFRLDVGDEEPRGGTITIEPVDDECAVTVVFRPTLKNTLTAE
                     ARAVVEGLGKNGYLDFEYVVKRFDLMKIMDEIRLAINHLNRFENRAFQNRDVAFSDGH
                     ALAAALLPLARIGHQLYTKLFSFGLNPGSMNVTSESLTRASWRVHGELLRSWLQDAKA
                     LHIVCRHCYLPWNLIYPELVADSAEAVRVEEFWGFSKRLQLFQVGVTNLAVMKSEWRL
                     AAGVEEKDGSVADGRRIHDDAAHPFRARANGRVSWHEQPRSLFADLERDIIYHFGHAS
                     GTTRGYAYNGVVLNGYSFSSADLETLFDTGQLPVTTASPHLLYLNGCSTAPGSGEGCN
                     STLVDALVRGSPGTFCFVTTFGDVPPWAAAEFARRFFDSYFGSSETAPRSLGDSLWIA
                     RHEMLALYQNPIGLLYEAVGKLATRLGAQPPSITGRLS"
     gene            complement(2081..4693)
                     /locus_tag="VF516_30585"
     CDS             complement(2081..4693)
                     /locus_tag="VF516_30585"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEX8112134.1"
                     /translation="MYVTKVLLDLVVLSAAYWLAQKLGPNAEQDAYWQFRVHVGWAVV
                     VLGEIVALRSLPVPSFTWRYVGVSGATSVALPLMSANLMFIIAHLAVAVFRQRTIPLG
                     VLLANTLLAIVGLISARLLFRLFRKRRDPRQVPSLDLTRVLLVGAGNVGMTVARELAA
                     RPEHGLQVVGFLDDDKWKIGTRIGGVRVIGTTAQIAEFAERLGATRVLITIANAPASE
                     IRRITLTCRDCGLGVKIVSGFSDVVNGRVSIREIALEDILACPRVEIDEPGVSDLVHK
                     AVVLVTGVGGSVGGELCRQLCAFAPRQVVLVERSDNALSELYNELSSQFRQVSFVPQL
                     ADVLDERRMDAVLAQSRPSLIFHTIDYRHVSMLEHFPTEAVKNNIGATHNMAELAHKR
                     RVGRFILVSDQKATNPISVMGATMRVAESMLQTLARHSTTRFICIRLGNLITHAGNVV
                     QIFRSQIARRGPVTVTHPEMARCFMHTPSACKLILQAASLGSSGDVISVDGGTPVKIL
                     DLARDLITLSGFQPDSDIQIRFTGVRAGEEIVEEPFDKASSRPTKHPLLWVNSDTQRL
                     SDPKAVDILLSAARSADNDDIRAQLQHLVPGFAQAGLQPTEKHRLFYVGNLPSTVTDT
                     GVRLACKDWGTIHDVQIPRNDAGRSRGFALVKVETPALGTPISSSYGATLAGRELRVD
                     EVRRADEDEVRRVTWQIPPRTRPTRQLQPAPSVSRVESDGAGTGAPLPVSPSLPAAST
                     DELVYLNASFDDGDDRLLVVGVPRTLLLDVGAPRAGSLLSPERGAFPRASRPPRSEKL
                     RFVVSSPLCRVTPATAVYEVGGPPARFELVARRGPKDGNMTVTISVLRDDALVHVASL
                     LLKVSGSQQPQVQP"
     gene            5083..5298
                     /locus_tag="VF516_30590"
     CDS             5083..5298
                     /locus_tag="VF516_30590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112135.1"
                     /translation="MENVAGMAGLRGLFESMRGPLSVTAFATQISGLYGGLVYLTPII
                     GGLVVDRALGRKMTVTIGAATMHRVIS"
     gene            5298..5561
                     /locus_tag="VF516_30595"
     CDS             5298..5561
                     /locus_tag="VF516_30595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112136.1"
                     /translation="MAFEMPFLLALLCLLIGVGCFKGNIASQVVTFSSRVCELPIGGC
                     AAPPDRGQRDIERRAGLRLMASLFPEHRVVSFRGCSFMARAFH"
     gene            5648..6385
                     /locus_tag="VF516_30600"
     CDS             5648..6385
                     /locus_tag="VF516_30600"
                     /inference="COORDINATES: protein motif:HMM:NF012925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HEX8112137.1"
                     /translation="MWSDEFHGSDGSRIDTAKWSAVTGGDGWGNQEHEYYMDDIATAQ
                     QRSGNLVITATRDGASAHHCWYGPCEYVSARLQTKGRFEQRHGRFEARIKVPRGQGLW
                     PAFWMLGNDFDSAGWPACGEIDIMENIGKEPGTVHGTLHGPGYSGSAGPTGRYELPGS
                     ARFADDFHVFAVEWESDAARFYVDDKLYETRTPTDLPPGTRWVFDHPFFLLLDVAVGG
                     VWPGNPDGNTVFPQTMLVDYVRVYRKA"
     gene            complement(6414..7898)
                     /locus_tag="VF516_30605"
     CDS             complement(6414..7898)
                     /locus_tag="VF516_30605"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8112138.1"
                     /translation="MGAPVVFFSYSRDDSDEDLNRFYVRLESEVAKLSGASMFRDTRN
                     VQLGTNWSDEIAKGLQHAKVMVCLFSRAYFASDWCGKEWTAFASRVANATGSPGNASP
                     MIPVVWNVVDMMPSVAQELQYTHDSLGGAYIRKGLRHLAQLQKPGAQDKFTKVIRVLA
                     EAIDAAIKQDLPPAQNPPDAMSLQNAFESPAKGEPHAEHGGRGSPGPRPSPEMEVRDK
                     RRVWNLRISRTLRISRRMIGSGIVIAILLAAIAIVTLYPRHDADLRHDAANPICGTYN
                     SLHAHAVGDEAVVMASDVGPLRYPDHGRIADFESGQGAEARTVFNQQISLLSDSAWRW
                     GQSKVWYERIDESGKDGPGILRLSFDLSSRDPKQPRYVGIYFDFSFPPPVSYDVSQFH
                     GIALSLKTSNIAMEVEVRIVLFSANICNSSYAFPTYIVPNEDLVDRWRKIKVRFDKFS
                     DPPWYRGHGLQLDPRRVYRVEIVVVSPKKDVEGYIELDDIRFVE"
     gene            complement(8113..>8910)
                     /locus_tag="VF516_30610"
     CDS             complement(8113..>8910)
                     /locus_tag="VF516_30610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112139.1"
                     /translation="RADGVSCAGCHVRGWVRRGPPAVAPTLLPLPDYPLITTGLYERS
                     DFCLPCHQLPPRDAVAGRPLLDTYREWLAGPYLPRGIQCQHCHMPNREHAVLGVHDPG
                     TFRQGVQLTAEAHRRAGTVTAVAALTNIGAGHALPTTPTPAAWLTITLLDARGQPIPG
                     AIDRYRIGRDIWFDGQWHERADTRIPPGETVTVARAWTAGRTAEATAARFTLEVHPDA
                     FYERFYAARLPHARDPAQRALYEQALARATRSHYVAEQRDVPIAPRP"
BASE COUNT         1648 a         2795 c         2858 g         1609 t
ORIGIN      
        1 ccgcgagccc tgtccggcat cctccgacag caggtgatcg gcgaggacgc gcttgccgaa
       61 ccgcgcaaag aaggccagga acgccgcggc caccgggggt ggaatcccgt cccagccacg
      121 atacttgagt gccagcagga cggcgcgcca ggttggatgc acgaggtcga tccacggcgc
      181 gaatgcttcg acgagattgt agaccgcctc catgaagaac gttccccagc agcgctcgag
      241 gcgctgttca ccgccgaacg cccggaacgg ctccggtggc cattgctgga actggttctc
      301 gaacgagagc tggtggccgt gttgcacatg gatcccacgg tcgcgtaaca cgcccttgtg
      361 aacgaagccg agctgtggtt cttcgcatcg caaggcactc gccagctggg accgaacacc
      421 gggccagtac agatcgacat catgatttcc ggcgaggagg aggagctgat cgccgcgctc
      481 gatgagcgcg cgaagcccct gaaacaccaa cggatggccc ttgatcgctc gcatcacctt
      541 ccggaccgat tcgtcttcgg tgctgccgat cgtcggatgc tcgagctcct tgtagcgagg
      601 cggaatcctt ggaaaatcga tgaaatcgcc gaggatgatc agcgttgcgg cctcgccgtt
      661 cgttcgatcc ggcagatagc tggaaagaaa cgcgcacagc tgttcgtccg aatgaaagtg
      721 atcgacttcg ccggccggat ctcccagatg cagatcgctg atcagaaaca catgaggatg
      781 gcggatctcg gtcatgacag acgacctgtg atcgacggtg gttgcgctcc caggcgcgtg
      841 gccagctttc ccaccgcctc gtacaagagg ccgatgggat tctggtaaag cgccagcatt
      901 tcgtggcgcg caatccacag actatcgccc aatgatcgcg gcgcggtctc cgaggagccg
      961 aagtacgaat cgaagaaccg tcgcgcaaac tccgcggcag cccacggcgg cacgtcgccg
     1021 aacgtggtca cgaagcagaa cgtgcccggg ctgccgcgaa cgagcgcgtc gaccagcgtg
     1081 gagttgcagc cctctcccga tcccggcgcc gtgctgcagc cgttcagata cagcaggtgg
     1141 ggggaagccg tggtgacagg cagctgaccg gtatcgaaca acgtctcgag gtcggccgac
     1201 gagaacgagt acccgttcag caccacgccg ttgtaggcgt atccacgcgt ggtccccgac
     1261 gcgtgaccga agtgatagat gatgtcgcgc tcgagatccg cgaacaacga acgcggttgt
     1321 tcgtgccagc tcacgcgtcc gttggctcgg gcgcggaatg gatgggcggc gtcatcgtga
     1381 atccggcggc catctgccac ggatccatcc ttttcctcga caccggcggc cagccgccac
     1441 tcggacttca tcaccgccag gtttgtgaca cctacctgga acaactgaag acgcttcgag
     1501 aatccccaga actcctccac ccgaacggct tccgcagaat ccgcaactag ctccggatag
     1561 atcaaattcc agggtaggta gcagtgacgg cacacgatgt gcaacgcctt ggcatcctgg
     1621 agccagctgc gaagcagctc gccatgaacc ctccagctgg cgcgagttaa cgactcgctc
     1681 gtgacgttca tcgaccccgg attgagacca aagctgaaga gcttcgtgta gagctgatga
     1741 ccgatcctgg ccagcggcag gagcgcggcc gcgagcgcat gaccatcgct gaacgcgaca
     1801 tctctgttct ggaaggcgcg attctcgaac cgattgagat gattgattgc caatcgtatc
     1861 tcatccatga tcttcatgag atcgaatcgt ttgacgacat actcgaagtc gaggtatcca
     1921 tttttcccaa ggccctcgac cacagcgcga gcctccgcgg tcaatgtatt cttgagtgtg
     1981 ggccggaaga cgaccgtgac cgcacactca tcatcgaccg gctcgatggt gatcgtgcct
     2041 ccgcgaggct cctcgtcgcc cacatccagt cgaaacccgt tcatggctgc acctgcggct
     2101 gctgcgaacc ggagaccttc aacaagaggc tcgcgacgtg aaccaaggcg tcgtcccgga
     2161 gaactgaaat cgtcactgtc atgttcccat ccttcggccc gcgtcgcgcg acgagctcga
     2221 agcgagctgg tggcccaccg acctcgtaaa ccgctgtcgc tggagttact cgacacaatg
     2281 gcgaagacac cacgaacctc agcttctcgc tgcgcggtgg ccgcgacgcg cgcgggaacg
     2341 cacctcgttc cggagatagc agacttccgg cgcgcggcgc tccgacatcg aggagcaagg
     2401 ttcgcggtac gccgacgacg agcaagcggt cgtctccgtc gtcgaacgag gcgttgagat
     2461 acacgagctc atcggtgctc gccgctggaa gtgacgggct cactggcaac ggagctcctg
     2521 ttccagctcc atcgctctcg acccgagata ccgacggtgc tggttgtaat tgccgggttg
     2581 gtctggtgcg cggcgggatc tgccatgtga cgcggcgcac ctcgtcctcg tcggcgcggc
     2641 gcacctcgtc gacgcgaagc tcgcgccctg ccagggttgc cccgtagctg ctggagatcg
     2701 gggtccccag cgctggagtc tcgaccttga ccagggcaaa gccacgcgaa cgcccggcgt
     2761 cattccgtgg gatctgaacg tcgtggatcg tcccccagtc cttgcatgcg agccgcactc
     2821 cggtgtccgt cacggtggat ggtagattgc caacgtagaa caagcgatgc ttctctgtgg
     2881 gctgcaaacc ggcttgcgca aaacccggga ccagatgttg taactgggct ctgatgtcat
     2941 cgttgtccgc tgaacgagcc gctgacagca ggatgtcgac ggccttgggg tcgctcagtc
     3001 gctgggtatc ggaattcacc cagagcagag gatgttttgt cggcctcgag ctcgctttgt
     3061 caaacggttc ctcgacgatc tcctcaccgg cccggacgcc cgtgaatcgg atctggatat
     3121 cgctgtccgg ctggaaaccc gacagcgtga tgagatcgcg tgcgagatcc aggatcttga
     3181 caggcgtgcc cccgtcgacc gagatgacat cgccgctgct gcccaggctg gctgcctgga
     3241 ggatcagctt gcacgcgctc ggcgtgtgca tgaagcagcg tgccatctcg ggatgagtca
     3301 ccgtgaccgg ccctctgcga gcgatctgcg aacgaaatat ctgaacgacg ttgccggcat
     3361 gcgtgatcag gttacccaga cgaatgcaga tgaaccgcgt ggtgctgtga cgcgcgagcg
     3421 tctgcagcat ggactcggcg acgcgcatcg tggcgcccat gaccgagatc ggattggtgg
     3481 ccttctggtc cgacaccagg atgaagcggc cgacgcggcg cttgtgagca agctccgcca
     3541 tgttgtgcgt ggcaccgatg ttgttcttga cggcttcggt cggaaagtgc tcgagcatcg
     3601 agacgtgcct gtaatcgatc gtatggaaga tcagggatgg ccggctctgt gcgagcacgg
     3661 catccatccg ccgctcatcc aggacgtcgg cgagctgggg cacgaacgaa acctgacgaa
     3721 actgactgga tagctcgttg tagagctcgg acaaggcatt gtccgagcgc tcgacgagca
     3781 cgacctgcct gggtgcaaac gcgcacagct gccggcacag ctcccctccg acgctgccac
     3841 cgacaccggt gaccagcacg accgccttgt gcacgaggtc gctcacgcca ggttcgtcga
     3901 tctcgacccg gggacacgcg agaatatctt cgagcgcgat ctcacgaata ctcacccgac
     3961 cattcacgac atccgagaac cccgagacga tcttcacccc caggccgcag tcgcggcagg
     4021 taagcgtgat gcgccggatc tccgaggccg gcgcattggc gatcgtgatc aggacgcgag
     4081 tggcaccgag gcgctctgcg aactcggcga tctgggccgt ggttccgatc acgcgaacgc
     4141 caccgatgcg cgttccgatc ttccacttgt cgtcgtccag gaagcccacg acctgaaggc
     4201 cgtgttcggg tcgcgctgca agctcgcgag cgaccgtcat ccccacgttg ccagcaccga
     4261 ccagcagcac acgggtaaga tccaaggaag gcacctgccg ggggtcgcgg cgcttcctga
     4321 acagccgaaa cagcaggcgt gcactgatga ggcccacgat cgcgagcagc gtgttggcga
     4381 gcagcacgcc gagcgggatc gtccgctggc ggaacacagc gacggccagg tgcgcgatga
     4441 tgaacatgag gtttgcgctc atcaggggca gcgcaacgct ggttgctccg ctcacgccca
     4501 cgtagcgcca ggtgaagctc gggactggca gcgatcggag cgccacgatc tcgccgagca
     4561 ccaccacggc ccatccaaca tgcacgcgga attgccagta tgcatcctgc tcggcgttgg
     4621 gcccgagctt ctgcgcgagc cagtacgcgg cggagagcac aacgagatcc aggagcacct
     4681 tggtgacgta caccgacgcc tgaaccctac gcgcgatcat cgttcctttg ccccctgtcg
     4741 ccctgactcg atgagctcac cacgccactg tccacctggc caccacatag accgcacaac
     4801 gatacaactc atggccgcat ccatgggcgc tgcaattcgc tgcatgtggc ggagcgtcgg
     4861 acggccgcgg tcagaccaag ggcgactgta cagcccgctg cgataaatac agtaacggca
     4921 ccgcgtgctg accacgctgc gcatccgggc gggatcgtgg ccgcccgggc ccaaggacgc
     4981 gctgatggct ggactcgacg acacgaccaa ggcgtggttc gaccagcagg tcgatccgca
     5041 gggcggcaag ggcaggacca tttcgcagga ggggcggggc atgtggaaaa cgtcgccggc
     5101 atggcaggtc ttcgcggcct gttcgaatcg atgcgcggtc cgctgtcggt cactgcgttc
     5161 gcgacgcaga tctcgggtct ctatggtggc ctggtctacc tgacgccgat catcggcggg
     5221 ctggttgtcg atcgcgcgct cggccgcaag atgacggtga cgatcggcgc cgccacgatg
     5281 catcgggtca tttcctgatg gcgttcgaga tgccgtttct gctcgcgctg ctgtgcctgc
     5341 tgatcggcgt cggatgcttc aagggcaaca tcgcgagcca ggtcgttacg ttcagcagtc
     5401 gggtgtgcga attgccgatc ggcgggtgcg cggcaccgcc cgatcgcggg cagcgcgaca
     5461 tcgagcgccg cgcgggtctt cggctcatgg cctcgctgtt cccggagcac cgcgtggtaa
     5521 gcttccgggg atgctcattc atggcgcggg cgttccattg atcgcggtga tgatatgtgc
     5581 ttgtgggacg gactcccgac cgaggggtgc gccggccgac aagcatctag tccctggctg
     5641 gcagctggtg tggtccgacg agttccatgg cagcgacggg tcgcgcatcg acaccgccaa
     5701 gtggtccgca gtcaccggtg gcgatggctg gggcaaccag gagcacgaat actacatgga
     5761 tgatatcgcg accgcgcaac agcgcagtgg gaacctggtc atcacagcga cccgggatgg
     5821 cgcatccgcc catcattgct ggtatggacc gtgcgagtac gtatcggcgc ggctgcagac
     5881 caagggacgt ttcgaacagc gtcatggccg attcgaagcg cgcatcaagg tccctcgcgg
     5941 gcagggcctg tggccggcat tctggatgct cggcaacgac ttcgacagcg ccggctggcc
     6001 ggcctgcggc gagatcgata tcatggagaa catcggcaag gagccgggca cggtgcacgg
     6061 cacactgcac ggccccggct actcggggag cgctgggccc actggccggt acgagctccc
     6121 cggctcggcc aggttcgccg acgacttcca cgtgttcgcc gtggaatggg agagcgacgc
     6181 cgcgcggttc tacgtcgacg acaagctcta cgagaccagg actcccaccg acctgcctcc
     6241 cggcaccagg tgggtgttcg atcacccctt cttcctgctg ctcgatgtcg ctgtcggcgg
     6301 cgtctggccg ggtaaccctg atggtaacac ggtgtttccg cagaccatgc ttgtcgacta
     6361 cgtgcgcgtt taccggaagg catgacagta ccgggcgccg tcgttgccgt cagctattcg
     6421 acaaaccgaa tgtcgtccag ctcgatatac ccctcgacat ctttcttcgg actgaccaca
     6481 acgatctcga ctcggtaaac acgcctggga tccaactgga ggccatggcc tctataccat
     6541 ggaggatcac tgaacttgtc gaacctgacc ttgatcttcc tccagcgatc gacgagatcc
     6601 tcgttcggaa cgatataggt cggaaatgca taactggaat tgcaaatgtt cgcagagaac
     6661 aatacgatcc ggacttctac ttccattgct atattgctcg tcttcaggct cagcgctatc
     6721 ccatgaaact gtgaaacatc atagctgacg ggaggtggaa atgagaagtc gaaatagata
     6781 cccacgtacc gcggttgctt gggatctctg gagctaaggt cgaaggaaag tcgcaagata
     6841 ccagggccat ccttgccact ctcgtcgatc cgttcgtacc acaccttcga ttgtccccac
     6901 cgccatgcac tgtcggagag cagtgagatc tgttgattga acaccgttcg agcctccgca
     6961 ccctggccgc tctcgaagtc cgcaatccgt ccgtggtcgg gataacggag tggccccaca
     7021 tccgaggcca tcacaacagc ttcgtcgccc acggcgtgcg catgtagact gttgtaagtc
     7081 ccgcagatgg ggttcgcggc gtcgtgtctg aggtcggcat cgtgtcgtgg gtacaaggtc
     7141 actatggcaa tcgccgcaag caggatggcg atgacgatac cggagccaat catgcgcctg
     7201 gatatgcgca gagtccgcga tatgcgcaga ttccacactc tcctcttgtc tctcacttcc
     7261 atctctgggc tggggcgggg ccccggactt cctcgcccgc cgtgttccgc atgtggttcg
     7321 cctttcgcag gcgattcgaa cgcgttctgg agcgacattg catccggcgg gttctgtgcc
     7381 ggcggcaggt cttgcttgat cgcggcgtcg atcgcttctg ccaggacgcg gatcaccttg
     7441 gtgaacttgt cctgagcgcc cggcttttgt aactgtgcca ggtgccgcaa gcctttccgg
     7501 atgtacgcgc cgcccagact gtcgtgcgtg tattgcagct cctgcgcgac cgaaggcatc
     7561 atgtccacga cgttccagac gacagggatc atcggcgagg cgttgccggg cgagccggta
     7621 gcgttcgcga cccggctggc aaacgcggtc cactccttcc cgcaccagtc tgacgcgaag
     7681 tatgcccgcg agaacaggca aaccatcacc ttggcgtgct gtaatccctt cgcgatctcg
     7741 tcggaccagt tcgtcccgag ttgcacattc cgtgtatccc ggaacatgct ggcgcctgag
     7801 agcttcgcga cctctgactc gagcctgacg tagaaccggt tcagatcctc gtcgctatcg
     7861 tctcgcgaat agctgaagaa cactactggc gcgcccactc cgtgtcatgt tacgctccat
     7921 ggatttcgaa gcgcaatccc tcgattgatc gtcttgcgac gccgcatcac cagcacacgg
     7981 tctggtatct tggcgaaaac gttttccggg gttactggtg ctcagcggga gtcctgcgat
     8041 gcgatcgagc aaggggatcc cgtgtggatg tggtccgctc ttggcggaat ttgccagggt
     8101 ggctataacc agctatggcc tcggcgcgat cggcacgtcg cgctgctcgg cgacgtagtg
     8161 ggagcgcgtg gcgcgggcca gcgcctgctc gtagagcgcg cgctgcgcgg gatcgcgggc
     8221 gtgcggcagc cgggcggcat agaaccgctc gtagaacgcg tcggggtgga cctcgagcgt
     8281 gaaccgcgcc gcggtggcct cggcggtgcg gccggccgtc cacgcgcgcg ccaccgtgac
     8341 ggtctcgccc ggcgggatcc gggtgtcggc gcgctcgtgc cactggccgt cgaaccagat
     8401 gtcgcggccg atccggtagc gatcgatggc gccggggatc ggctggccgc gcgcgtcgag
     8461 cagggtgatg gtgagccacg cggccggggt cggggtcgtg ggcagggcgt ggccggcgcc
     8521 gatgttggtg agcgcggcga cggcggtgac ggtgccggcg cggcggtgcg cctcggcggt
     8581 cagctgcacg ccctggcgga acgtgccggg atcgtggacg ccgagcacgg cgtgctcgcg
     8641 gttgggcatg tggcagtgct ggcactggat gccgcgcggc aggtacgggc cggcgagcca
     8701 ttcgcggtag gtgtcgagca gcggccggcc ggcgacggcg tcgcgcggcg gcagctggtg
     8761 gcacggcagg cagaagtccg agcgctcgta cagtccggtg gtgatgagcg gatagtcggg
     8821 caagggcaag agcgtcggcg cgaccgcggg cggaccgcgg cggacccagc cgcgcacgtg
     8881 gcagcccgcg cacgacacgc cgtcggcgcg
//
LOCUS       DASVOR010001633         3184 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_396009,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001633 DASVOR010000000
VERSION     DASVOR010001633.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396009"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="VF516_30615"
     CDS             complement(<1..1143)
                     /locus_tag="VF516_30615"
                     /inference="COORDINATES: protein motif:HMM:NF016032.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HEX8112140.1"
                     /translation="MHLVDEQDAVRVRSTDDLLDYFETACKPASNWRVGTEHELVGVL
                     TGADALGEPPTYDGSHGIGALFQWFAARSGTPVLEDGHMIALSRGDAQLTIEPGGQFE
                     LAARPVADDRDFAADLTAYVTELGAASRELGLAWLATGLRPFGTRDDIPWMPKQRYAV
                     MRNYMPTVGTRGLDMMLRTATVQANLDFSDEADAAAKLRCLYSVTSLLTALWACSPIV
                     EDRVSGYQSYRAWIWRDTDNARAGLVPFVFERSDVFRAYTEWALDVPMYFLYRGGYHR
                     VPAGFTFRRFLREGLGDQHALRADWALHLSTLFPDGRLKKFIEVRGCDCGSLGMIAAL
                     GPMMRGLLYDPAARTAATALTAELSFADRQRLADDVPRLGFAARAGK"
     gene            1216..1806
                     /gene="pyrE"
                     /locus_tag="VF516_30620"
     CDS             1216..1806
                     /gene="pyrE"
                     /locus_tag="VF516_30620"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEX8112141.1"
                     /translation="MQSLPDRSALSVKRERLLELLRSRAFQEREVVLSSGRTSNFYID
                     CKQVSLDAEGATLIGELFHAVIEEVAPQALAVGGLTLGADPLATATSVVSFLAGRPRA
                     AFLVRKEPKGHGTHQWLESTRLPAGAEVVVVEDVVTTGAATLKAIERARLAGLRVVHA
                     LGLVDRLEGGREAVIREVPLTTLFTRRDFLPDSPQA"
     gene            1917..2462
                     /locus_tag="VF516_30625"
     CDS             1917..2462
                     /locus_tag="VF516_30625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112142.1"
                     /translation="MRLTEDWPATPRDYASVVRDWTRKAQLHASYQEVCELAATLKSA
                     EWRAALARHDADIRGLVGEGREQRIAQSRAEVAGPYELEIMLTTWDRRENDLDRGKKS
                     VWRVVLIDETGQEIEPLEIVRDRRPAFVVRAEFPSLGEFATPYIARFPRTRPIFGPGA
                     RQVRLRMSSPRGGLELDWTSS"
     gene            complement(2485..>3184)
                     /locus_tag="VF516_30630"
     CDS             complement(2485..>3184)
                     /locus_tag="VF516_30630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112143.1"
                     /translation="TRPERPGAVGKPATASGSPPAADPVASDAVVIEDVPDAVASDAI
                     TAPIALVSPPDDAAAPDPADAADSTPTAVVDEPSDGVVRHHIATAETAPVKRRQLPHD
                     PRDDDRPGETTGEITMPRAQPQVVIRYSEPTILVPDLAAIQAEVGEAGPAGSASHPAA
                     GGAHGGPGFSDAEEAFFRAGHDKAAGHHDAESFDDLDEGYRPVGFWERLTGRGKPGKP
                     DRSGKPSKKPKPDK"
BASE COUNT          423 a         1139 c         1159 g          463 t
ORIGIN      
        1 cttgccggcg cgcgccgcga aaccgagccg gggcacgtcg tcggcgagcc gctgccggtc
       61 ggcgaacgac agctcggcgg tcagcgcggt cgcggcggtg cgcgccgccg gatcgtagag
      121 cagcccgcgc atcatcggcc cgagcgcggc gatcatcccg agcgagccgc agtcgcagcc
      181 gcggacctcg atgaacttct tgagccggcc gtcggggaac agcgtcgaca ggtggagcgc
      241 ccagtcggcg cgcagcgcgt gctggtcacc caggccctcg cgcaggaacc ggcggaacgt
      301 gaagccggcc gggacgcggt ggtagccgcc gcggtacagg aagtacatcg gcacgtcgag
      361 cgcccactcg gtgtatgcgc ggaacacgtc gctgcgctcg aacacgaacg gcaccagccc
      421 ggcgcgcgcg ttgtcggtgt cgcgccagat ccacgcccgg tagctctggt agccggacac
      481 ccggtcctcg acgatcggcg agcacgccca cagcgcggtg agcagcgagg tcaccgagta
      541 caggcagcgc agcttggccg cggcgtcggc ctcgtcggag aagtcgaggt tggcctgcac
      601 cgtcgcggtg cgcagcatca tgtcgagccc gcgggtgccg acggtcggca tgtagttacg
      661 catcaccgcg tagcgctgct tgggcatcca cgggatgtcg tcgcgggtgc cgaacgggcg
      721 caggccggtc gccagccagg ccaggccgag ctcgcgcgac gcggcgccga gctcggtgac
      781 gtaggccgtc agatcggcgg cgaagtcgcg gtcgtcggcc accggccgcg ccgcgagctc
      841 gaactgaccg cccggctcga tcgtcagctg cgcgtcgcct cgcgacagcg cgatcatgtg
      901 gccgtcctcg agcaccggag tgccgctgcg cgcggcgaac cactggaaca acgcgccgat
      961 cccatgtgat ccgtcgtacg tcggcggctc ccctagagca tcggcgccgg tcaggacgcc
     1021 gaccagctcg tgctcggtcc cgacccgcca gttcgacgcc ggcttgcacg ccgtctcgaa
     1081 ataatcgagc agatcgtccg tcgagcgaac ccggacggca tcttgttcgt cgacgagatg
     1141 cacgtggttc agtgactcta gggggtgatc cccatggcgt caatcggctc gcggcacatg
     1201 gtagcgtttg tgaggatgca gtccctaccc gatcggtctg cgctctcggt caagcgcgag
     1261 cgcctgctgg agctcctgcg cagccgcgcg ttccaggaac gcgaggtcgt gctgtcctcg
     1321 ggtcggacct ccaacttcta catcgattgc aagcaggtca gcctcgacgc cgagggcgcg
     1381 accctgatcg gcgagctgtt ccacgccgtg atcgaggagg tcgcgccgca ggcgctcgcg
     1441 gtcggcggct tgaccctcgg tgccgatccc ctcgccacgg cgacgtcggt ggtcagcttt
     1501 ctcgcaggcc gtccgcgcgc ggcattcctg gtgcgcaagg agcccaaggg gcacggcacc
     1561 caccagtggc tggagagcac ccggctcccg gccggcgccg aggtcgtcgt cgtcgaggac
     1621 gtcgtcacca ccggcgccgc gaccctcaag gcgatcgagc gcgcgcggct cgccggtctg
     1681 cgcgtcgtgc acgccctcgg cctggtcgat cgcctggagg gtgggcgcga ggcggtgatc
     1741 cgcgaagtcc cgctgaccac gctgttcacg cggcgcgatt tcctgccaga ctccccgcaa
     1801 gcctgatcat gaggcgcgcc ggcatcatct ggggacgacc taccgcagcg gcggcacggg
     1861 tcactgtgct ggtcactgtg ttgctcgggg cgtcctgtac ggtgagctat cctacggtgc
     1921 gcctgaccga ggactggccc gcgacccccc gcgactacgc cagcgtcgtc cgcgactgga
     1981 cgcgcaaggc ccagctccat gccagctacc aggaagtctg cgagctggcc gccaccctca
     2041 agtcggcgga gtggcgcgcg gcgctcgccc ggcatgacgc cgacatccgc ggcctggtcg
     2101 gcgagggccg cgagcagcgc atcgcgcagt cgcgcgccga ggtggccggc ccctacgagc
     2161 tcgagatcat gctcacgacc tgggatcgcc gcgagaacga cctcgatcgc ggcaagaaga
     2221 gcgtgtggcg ggtcgtgctg atcgacgaga ccggccagga gatcgagccg ctggagatcg
     2281 tccgcgaccg ccggcccgcg ttcgtggtgc gcgccgagtt cccctcgctc ggcgagttcg
     2341 cgacgcccta catcgcgcgg ttcccgcgca cccggccgat cttcggcccc ggcgccaggc
     2401 aggtgcggct ccggatgagc agcccgcgcg gcggcctcga gctcgactgg acgtcgagct
     2461 gacgcaccac ggctccggcg cgggctactt gtcgggcttg ggcttcttgc tcggcttgcc
     2521 gctcctgtcc ggcttgcccg gcttgccccg gccggtcagg cgctcccaga acccgacggg
     2581 tcggtagccc tcgtcgaggt cgtcgaacga ctcggcgtcg tggtgcccgg ccgccttgtc
     2641 gtggccggcc cggaagaacg cttcctcggc gtcgctgaac ccggggccgc cgtgcgcgcc
     2701 gccggccgcg gggtgcgacg cggatcctgc cgggcctgcc tcgccgacct cggcctggat
     2761 cgcggcgaga tccggcacca ggatcgtggg ctcgctgtac cggatcacga cctgcggctg
     2821 ggcgcgcggc atcgtgatct cgccggtcgt ttcgcccggg cggtcgtcgt cgcgcggatc
     2881 gtgggggagc tggcggcgct tgaccggcgc ggtctcggcg gtcgcgatgt gatggcggac
     2941 cacgccgtcg gacggctcgt cgacgaccgc ggtcggcgtg gagtcggcgg cgtccgcggg
     3001 atccggcgcc gccgcgtcgt ctggcggcga caccaacgcg atcggcgcgg tgatcgcgtc
     3061 gctggcgacc gcgtcgggga catcctcgat gaccaccgcg tccgacgcta ccgggtccgc
     3121 ggccggcggc gatccggagg ccgtcgcggg cttgcccacg gcgcccgggc gctcgggccg
     3181 ggtg
//
LOCUS       DASVOR010001634         2320 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_337857,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001634 DASVOR010000000
VERSION     DASVOR010001634.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2320
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337857"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="VF516_30635"
     CDS             complement(<1..217)
                     /locus_tag="VF516_30635"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8112144.1"
                     /translation="MRGLNLDYLRAFVDVIERGSFSAAARGLGLTQPAVSLQVRQLEA
                     RLGVRLVERVGRTARPTAAGAELLEHAR"
     gene            292..879
                     /locus_tag="VF516_30640"
     CDS             292..879
                     /locus_tag="VF516_30640"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8112145.1"
                     /translation="MSRSAELPVAVREARAADMAAVQAIYAHHVLHGLATFEEVPPSV
                     DELIERRAAVVGAGLPYLVAEHAGAVVGYSYASAYRPRPAYRHAIEDSVYVADGLAGR
                     GIGAALLAALIARCEAGPWRQMIAIIGDTGNAASIALHRRHGFGLVGTLHAVGFKLGR
                     WVDTVLMQRALGPGAAAPPGNDRGNDGANHQVVAP"
     gene            876..2081
                     /locus_tag="VF516_30645"
     CDS             876..2081
                     /locus_tag="VF516_30645"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8112146.1"
                     /translation="MIAGRTVLGLGLSQLILWGITYYLLGGFGDAMTADLGASRDAVY
                     GGFSAALLVMALASPLAGRWIDRHGGRRVMAAGSVLNAAGCLALAASRSLWAYYAAWL
                     ALGVAMRLTLYDAAFAALARIAGAGARRAMAQITLLGGLASTVFWPLGHWMAGQVGWR
                     GAVAAYAGFALLTVPLHLAIPETRHEPHVAAAAAGSGRPVATGRGDRILASVLYAATA
                     ASAAFLNAGMSSHMIGMLTGLGVAASAAVWIATLRGVGQSAARLSEVVFGRRIDPLTL
                     NLAASALLPACFIAGLFSGRSTAAAAGFALVYGAGNGILTITRGTLPLVLFDPRVYGA
                     YVGKLLVASFVLSAAAPVVYASAIQRLGEAGALGMSLAAAGLVLAAAVVLRLRYQGRA
                     NLGSPGHDM"
BASE COUNT          257 a          832 c          871 g          360 t
ORIGIN      
        1 ggcgcgcgtg ctcgagcagc tcggcgcccg cggcggtggg ccgcgccgtg cgcccgacgc
       61 gctcgaccag ccggacgccg agccgggcct cgagctggcg gacctggagg ctgaccgcgg
      121 gctgggtcag gccgaggccg cgcgcggcgg ccgagaagct gccgcgctcg atcacgtcga
      181 cgaacgcgcg caggtagtcg aggttgagcc cgcgcatcgc aaagaatctc tcatgctcag
      241 catgattttc agagtcttct tttatcgctg gagatccgcc acgatcgcgg catgtcccgg
      301 tcagcggagc tccccgtcgc ggtgcgcgag gcccgcgccg ccgacatggc ggcggtccag
      361 gcgatctacg cccaccacgt gctgcacggc ctggcgacgt tcgaggaggt gccaccgtcg
      421 gtcgacgagc tgatcgagcg ccgggcggcg gtggtcggcg ccgggctgcc ctacctggtc
      481 gcggagcacg ccggcgcggt cgtcgggtac agctacgcgt cggcgtaccg gccgcggccg
      541 gcctaccgcc acgcgatcga ggactcggtc tacgttgccg acggccttgc cgggcgcggc
      601 atcggcgcgg cgctgctcgc ggccctgatc gcgcgctgcg aggccggtcc gtggcggcag
      661 atgatcgcga tcatcggcga caccggcaac gcggcgtcga tcgcgctgca ccggcggcac
      721 ggctttggcc tggtcggcac gctgcacgca gtgggcttca agctcggccg ctgggtcgac
      781 accgtgctga tgcagcgcgc gctggggcct ggagccgccg caccgccggg caacgaccgg
      841 ggcaacgacg gggcaaacca ccaggtggtg gcgccttgat cgccggtcgc accgtgctcg
      901 gcctcgggct gtcgcagctg atcttgtggg gcatcaccta ttatttgctc ggcgggttcg
      961 gcgatgcgat gaccgccgac ctcggcgcga gccgcgacgc ggtctacggc gggttctcgg
     1021 ccgcgctgct ggtcatggcg ctcgcgtccc cgctcgccgg ccggtggatc gatcgccacg
     1081 gcggccgtcg cgtgatggcc gccggatccg tgctcaacgc cgccggctgt ctggcgctcg
     1141 ccgcgagccg cagcctctgg gcctactacg cggcgtggct cgcgctcggc gtggcgatgc
     1201 ggctcaccct ctacgacgcc gcgttcgccg cgctcgcgcg gatcgccggg gccggggcgc
     1261 gccgcgcgat ggcgcagatc acgctgctcg gcgggctggc ctcgacggtg ttctggccgc
     1321 tcggccactg gatggccggg caggtgggct ggcgcggtgc ggtcgccgcg tacgccgggt
     1381 tcgccctgct caccgtaccg cttcacctcg cgatccccga aacccgccac gagcctcacg
     1441 tcgccgcggc ggcggctggc agcgggcggc ccgttgccac cgggcgcggc gaccggatcc
     1501 tcgccagcgt cctgtacgcc gcgaccgcgg caagcgccgc gttcctcaac gccggcatgt
     1561 cgtcgcacat gatcggtatg ctcaccgggc tcggcgtcgc ggcctcggcc gccgtgtgga
     1621 tcgccacgct gcgcggcgtc ggccagtcgg ccgcccggct gagcgaggtc gtgttcggcc
     1681 ggcgcatcga tccgctgacc ctcaacctcg cagcgtccgc gctgcttccg gcgtgcttca
     1741 tcgccggcct gttcagcggg cggtcgaccg ccgcagcggc cggcttcgcg ctggtctacg
     1801 gcgccggcaa cggcatcctg accatcacgc gcggcacgct gccgctcgtg ctgttcgatc
     1861 cgcgggtcta cggcgcgtac gtcggcaagc tgctggtcgc cagcttcgtg ctctcggcgg
     1921 cggccccggt ggtctacgca tcggcgatcc agcggctcgg cgaggccggc gcgctgggga
     1981 tgtcgctggc ggccgccggc ctcgtgctgg ccgcggcagt cgtgctgcgg ctgcggtatc
     2041 aaggtcgagc caatctcggc tcacctggtc acgacatgtg accaggcaca tgcttttttc
     2101 gtcgagccgg gggatggggt gcgcgcggcg ggtcatgctg gcctcgtaac cgtgggagct
     2161 gcctggtgtc ttcgcacgtg agctcggggg gaacaggctg ccccggccgg gcttcggtct
     2221 gcaccaaagt ggagccgcgc gcccgccggt gctaggtaga gagacggtgt cgcgacgacg
     2281 acagctcgtg gtcctggccg cgtgggtggt ggccgacggc
//
LOCUS       DASVOR010001635         6561 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_46283,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001635 DASVOR010000000
VERSION     DASVOR010001635.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46283"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF516_30650"
     CDS             <1..210
                     /locus_tag="VF516_30650"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8112147.1"
                     /translation="GVSGSHPGSPGLPGPASSGDDELDRAAIEAALARAGGVIAQAAA
                     ELGLSRQALYRRMEKLGVSTSPSVR"
     gene            457..1539
                     /locus_tag="VF516_30655"
     CDS             457..1539
                     /locus_tag="VF516_30655"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8112148.1"
                     /translation="MQIFDPDTQSPQHAAWQPTSQLPPALALRQLLFGHRVTRIIAVA
                     AQLKLADAMDETPRSVAALAAAVGADRAALQRLLYALASIGLVTTSAPGQFALTPVGA
                     CLRSDAPHGLRSWALMESAEYYQAAWDHLLPGMRSGAPTFESAVGQSFYDYLDRHPVD
                     GANFSQTMQEVTAVIVDAVLAAYDFASVERIVDVGGGYGRLLTSLLRRYPAMRGVLLD
                     TPAVIARAKPQIQATGVADRCELVGGDFFADLPAGGDLYLLSRILMDHDDEASVRLLQ
                     NCRRAMTGRGRVHIVQIVLPSSEADAARPLLFDAAMSNLNMFVLGLGAERTEEQYRAL
                     LAGAGFTVTQIIPTRALMSIIEARPT"
     gene            1611..3431
                     /locus_tag="VF516_30660"
     CDS             1611..3431
                     /locus_tag="VF516_30660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112149.1"
                     /translation="MPSPSIAELVTAACTGSPDRWVQRLPAPAQPLVRRALDLDRPLW
                     VQYPASLPSCLLARTLGSGELGSLPIDWMRELDELGAPWIRPLRPLPVKDGLLAELHD
                     AEHLALAEGGTLSFASEDEVCVDRYAHTRPEATRRLRWSWTRSEIAFGPEVGAERPDR
                     DGYPRIEKAGWGPAFVILSPGAARVELPSPPESNADARIMADGARVAVYGALEDYEGG
                     FVHIVDLATMAIERSLTTRRTVSNVHECARRDLLLVATYGGLVAWRGDLARELPIGRG
                     RSACLSPSGAHIAVLDDSLRLWALETVMQSEAGHASRGFPTCFDPQGDRLLSGSSLFE
                     ARSGRRVATLHPDLGPYLEGGPASPWMHLGTQYLVSLHGGLAVWDAHTGEALRVEESC
                     HYPQWYSVAYDHTGARLAALRHDSTQVVLHALPSGRISHEIGFDLDGSAIAISPDGEL
                     LAIQCGGAVEVRAVNGTRVQRYGSPGTPPSADRPAWRRGPPQRPDAALAGRYGPPTLQ
                     FSRDGRRIARFTPDDGWRVWTLGDHAAEHASAETPLDDIPDFSPPHPADWTLEADSKT
                     VFTHHPTGTRIALPAAGPWSANPAVPRILASDDLHIELRG"
     gene            complement(3474..4841)
                     /locus_tag="VF516_30665"
     CDS             complement(3474..4841)
                     /locus_tag="VF516_30665"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8112150.1"
                     /translation="MCDAPERHNWKGDLSYVPCVVAQAHSVDDIVRIVKDRERYPSPV
                     RAAGSQHSTTLCIEANGGTIVDVTKLDRILAIDDQALTITMQPGVRYVDAARALEKVG
                     LAFHSNVEIGNSTVGSLACCSTKESSFPGAYSQASSYLIAARMVLPSGEIREVDEREP
                     ELLRVVRSSFGLLGILCEVTFRVRKLQALEVCHVSYSLHAFADHLPRLIAEAQQTDRS
                     FMMYQFPHIDRVVVEYLRYADGPIRSHWQWRLRNWLWRTVAPGFGRLVTDNVPTRWLR
                     DRLYNTFERLCVAALTVAIRGKATSPPDQIVRYAETAGYAAMTFSIWTFPEHEFSETI
                     RAYFQFCKDYYRQHGFRCDMPNGGYLVAKDTRSLFSYSSKEAMLTIDPATNGIAGWNA
                     FLVAYNAFAIARHGRPLINLTTWLTHDQARIAFGEEMAVFQKFRRQFDPTDRLYSGFF
                     RALFG"
     gene            5114..6397
                     /locus_tag="VF516_30670"
     CDS             5114..6397
                     /locus_tag="VF516_30670"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate 2-monooxygenase"
                     /protein_id="HEX8112151.1"
                     /translation="MAIAMVPIQNIGPARQQEVYVCGVAGRRPLIPVAQRKLEEAAHK
                     VLPPKSWSYIAGSAGRESTARANEAAFERWRIVPRVLHDVSVRDLGIDLFGQRLPAPV
                     LLAPIGVLETAHRQADLAVARAAAAIGVPFVFSSQASVNMETCAAAMGDSPRWFQLYW
                     SRSNEVVKSFVRRAEACGCSAIVLTLDTTLLGWRPRDLDLGSLPFLRGMGLAQYTTDP
                     AFLAELHEPLDVPVMQPPLTLSTLRVALEQKKRFPGGLLRNLFSDEPRLAVQRFVATY
                     SRPSLQWEDLALLRSFTRLPILLKGVLHPDDARRAVEAGMDGVIVSNHGGRQVDGAIA
                     ALDALRGIVDAVQARVPVLFDSGIRGGADVFKALALGARAVLLGRPYVYGLALAGERG
                     VRDVILNVLAELDLTMGLSGCRNLQEVTREMLARA"
BASE COUNT          955 a         2291 c         2266 g         1049 t
ORIGIN      
        1 ggcgtctccg gatcccaccc cggatcgccg ggcctgccgg gccccgcgag ctccggcgac
       61 gacgagctgg atcgcgctgc gatcgaggcc gcgctcgccc gcgccggcgg cgtcatcgcc
      121 caggccgccg ccgagctcgg cctgagccgc caggcgctgt accggagaat ggaaaagctc
      181 ggcgtctcca cgagcccttc ggtccgctag gatcggccgc cgccgtacca gccccgtggc
      241 acatgcatgg cacacgcggc acggatgtgc cgccggatcg ccggcctggt gacggagcac
      301 ggcgctctcg agcaccaccg ccctgctcgc agggagcaat ccatcaagga atgagggcga
      361 gtgggagtgg ggcctgatgg cgtgcggggg tctgcttcac cggtccgggg cctgcatggt
      421 cgaggtcgtc cgcggtggaa acaggaggga atctcgatgc agatcttcga tccggatacg
      481 caaagtccgc aacacgcggc ctggcaaccg accagtcagc tccccccggc cctggccttg
      541 cggcagctgc tcttcgggca ccgggtgacg cgcatcatcg ccgtggccgc ccagctgaag
      601 ctggccgacg cgatggacga gacgccgcgc agcgttgccg ccctggccgc cgccgtcggg
      661 gccgatcggg ctgccttgca gcggttgcta tatgccctcg ccagcatcgg cctcgtgacc
      721 acatccgcgc caggtcaatt tgcactgacc ccggtgggcg cgtgcctgcg cagcgatgcc
      781 ccccacgggc tgcgctcgtg ggcgctgatg gagagtgccg agtattacca ggcggcgtgg
      841 gatcacctct tgcccggtat gcgcagcggg gcacccacct tcgaatccgc ggtcggtcag
      901 tcgttttacg actacctgga tcgacacccc gtggacggcg cgaacttcag ccagacgatg
      961 caagaggtca ccgccgtgat cgtcgacgcc gtgctggctg cctacgactt tgcgtccgtg
     1021 gagcggatcg tcgacgtcgg cggcggctac ggcaggctgt tgaccagcct cctgcggcgc
     1081 tatcctgcga tgcgcggtgt gctcctcgac accccggccg tgatcgcgcg ggccaaaccc
     1141 cagatccagg ccacgggagt tgccgatcgc tgcgagctgg tgggcgggga cttctttgcc
     1201 gacttgccgg cgggtggcga tctctacctg ctatcgcgca tcctgatgga ccacgacgac
     1261 gaagccagtg tgcgtcttct gcagaactgc cgccgggcga tgacggggcg ggggcgagta
     1321 cacatcgtcc agatcgtgct gccgtcgtcc gaagcggacg ccgcacgccc cctgctgttc
     1381 gatgccgcca tgtccaacct caacatgttc gtgctgggtc tgggcgcaga gcggacggaa
     1441 gagcagtatc gcgctctgct cgcgggggcg ggcttcacgg tcacgcagat catcccgacc
     1501 cgcgccttga tgagcatcat cgaggccaga ccgacgtagc cgcgtagatc tgcagcgttc
     1561 cggactttgc gcttgacggt cggcgcggcg gcttcgacga tcgaacggcc atgccctcgc
     1621 cgtcgatcgc agagctcgtg accgccgcct gcacgggctc gcccgatcgc tgggtgcaac
     1681 gtctgcccgc gccggcgcag cccctggtcc gccgcgcgct ggatctcgat cggccgctgt
     1741 gggtgcaata tcccgcctcg ctcccgagct gcctgctcgc gcgaacgctc ggatccggcg
     1801 agctcgggtc gctgccgatc gactggatgc gcgagctcga cgagctcggc gcgccctgga
     1861 tccgcccgct gcgcccgctc ccggtgaaag acggcctgct cgccgagctg cacgacgcgg
     1921 agcatctggc gctcgcggag ggcggcacgc tgtcgttcgc gtccgaggac gaggtgtgcg
     1981 tggaccggta cgcccataca cgccccgagg cgacgcgccg cctgcgctgg tcctggacgc
     2041 gcagcgagat cgcgttcggt cccgaggtcg gagcagaacg tccggaccgc gacggctatc
     2101 cccggatcga gaaggccggc tggggcccgg cgttcgtgat cctgtcgccc ggcgccgcca
     2161 gggtcgagct gccgtccccg ccggagagca acgccgacgc ccgcatcatg gccgacggtg
     2221 cgcgcgtggc ggtctacggc gcgctggagg actacgaagg tgggttcgtt cacatcgtcg
     2281 acctcgccac gatggcgatc gagcgctcgc tcacgacgcg ccgcacggtc agcaacgtcc
     2341 acgagtgcgc gcgccgcgat ctgctgctgg tcgcgaccta cggtgggctg gtcgcgtgga
     2401 gaggggatct cgcgcgcgaa ctgcccatcg gccgcggccg cagcgcgtgc ctgtcgccca
     2461 gcggggcgca catcgccgtc ctcgacgaca gcctccgcct gtgggcgctg gagaccgtga
     2521 tgcagagtga agccgggcac gcttcccgcg ggtttcccac gtgcttcgat ccgcagggcg
     2581 accgtctgct gtccggaagc tcgctgttcg aagcgcgcag cggccggcgc gtggcgacgc
     2641 tgcatcccga ccttggcccg tacctcgagg gcggcccggc gtcgccctgg atgcacctcg
     2701 ggacgcagta cctggtgtcg ctccacggcg gcctcgcggt ctgggacgcc cacaccggcg
     2761 aggccctgcg ggtcgaggag agctgccatt atccacagtg gtactcggtt gcctacgatc
     2821 acaccggcgc ccggctcgcc gcgctgaggc acgattcgac gcaggtcgtg ctccacgcgc
     2881 tgccgagcgg ccggatcagc cacgagatcg gtttcgacct cgacggctcc gccatcgcga
     2941 tcagcccgga cggtgagcta ctcgcgatcc agtgcggagg cgccgtcgag gtccgagcgg
     3001 tgaacggcac gcgggtgcaa cggtacggat ctcccggcac gccgccgtcg gcggatcgtc
     3061 cggcgtggcg ccgcggaccg ccgcaacgcc ccgatgcagc gctcgctggc cggtacgggc
     3121 cgccgacctt gcagttctcg cgagatggcc ggcggatcgc ccggttcacc cccgacgatg
     3181 gctggcgggt ctggacgctc ggggaccacg ccgccgagca cgcctccgcc gagacgccgc
     3241 tcgacgacat ccctgacttc tctccgccac acccggccga ctggaccctc gaggcggact
     3301 cgaagacggt attcactcac catcccaccg gcacgcggat cgcgctgccg gccgcaggtc
     3361 cgtggtccgc aaaccctgcg gtgccgcgga tcctcgcctc cgacgacctg cacatcgagc
     3421 tgcgcggatg accgggtagc gagtccgtac cgggtcttcg ccgcgggcca ccctcatccg
     3481 aagagcgcgc ggaagaaccc cgagtacagc cggtccgtgg gatcgaactg gcggcggaac
     3541 ttctggaaca ccgccatctc ctcgccgaag gcgatccggg cctggtcatg cgtgagccag
     3601 gtggtcaggt tgatcagcgg ccggccgtgg cgggcgatgg cgaacgcgtt gtacgcgacg
     3661 aggaatgcgt tccatccggc gatcccgttg gtcgccggat cgatcgtcag catcgcctcc
     3721 ttgctggagt acgagaacag cgagcgggtg tccttcgcga ccaggtagcc gccgttcggc
     3781 atgtcgcagc ggaacccgtg ctgcctgtag tagtccttgc agaactggaa gtacgcgcgg
     3841 atcgtctcgg agaactcgtg ctcggggaac gtccagatgc tgaaggtcat cgccgcgtag
     3901 ccggcggtct ccgcgtaccg gacgatctgg tcgggcgggc tggtcgcctt gccccggatc
     3961 gcgacggtga gcgccgcgac gcacaaccgc tcgaacgtgt tgtacagccg gtcgcggagc
     4021 cagcgggtcg ggacgttgtc cgtcaccagc cgcccgaacc ccggcgcgac ggtgcgccac
     4081 agccagttgc gcagccgcca ctgccagtgg ctccggatcg ggccgtcggc gtatctcagg
     4141 tactcgacca cgaccctgtc gatgtgcggg aactgataca tcatgaacga gcgatcggtc
     4201 tgctgggcct cggcgatcag gcgaggcagg tggtcggcga atgcatgcag cgagtacgag
     4261 acgtggcaga cctccagcgc ctgcagcttg cggacccgga aggtgacctc gcacaggatg
     4321 ccgagcaggc cgaagctgga gcgcaccacg cgcagcagct cgggctcgcg ctcgtcgacc
     4381 tcgcggatct cgcccgacgg caggaccatc cgcgcggcga tcaggtacga cgacgcctgg
     4441 ctgtaggcgc cgggaaacga ggactccttg gtgctgcagc acgccaggct gccgaccgtg
     4501 gagttgccga tctcgacgtt gctgtggaac gccaggccga ccttctcgag cgcccgggcc
     4561 gcgtcgacgt accgcacgcc gggctgcatc gtgatcgtca gcgcctggtc atcgatcgcg
     4621 aggatcctgt ccagcttcgt gacgtcgacg atcgtaccgc cgttggcctc gatgcacagc
     4681 gtcgtcgagt gttgcgatcc ggcggcgcgg accggagacg gatagcgctc ccggtccttc
     4741 acgatccgca cgatgtcgtc cacgctgtgc gcctgtgcca cgacacacgg gacgtagctg
     4801 agatcgcctt tccagttatg ccgctcgggc gcgtcgcaca tgggccttgc ggccgacgtc
     4861 tcgagagttt gctgccccac gtcccttcca cctttccgcc gaccagcgct cacttcaatg
     4921 tcgagtcacc gtagcagctg gtgaactacg ggcaagcgcg atccgtggca ttgacatgca
     4981 atgtgacgta cctgggcgga cagccgcctc acgtagactg cgtcgacggg ccgcagcgtt
     5041 ggcgccgacg gcggcggtgg atcctgatcc tgccgcgatt ggatccttcc catcccgttg
     5101 ccagaccata cccttggcga ttgccatggt cccgattcag aacattggcc ctgcgcggca
     5161 gcaggaagtg tacgtctgcg gtgtggccgg gcgccgtccc ctgatacccg tggcgcagag
     5221 gaagctggag gaggcggcgc ataaggtcct gccgccgaag tcctggtcct acatcgcggg
     5281 cagcgcggga cgggagagca ccgcccgcgc caacgaggcc gcgttcgagc gctggcgcat
     5341 cgtgccgcgg gtgttgcacg acgtctccgt gcgcgacctc ggcatcgacc tgttcggcca
     5401 gcgtctgccg gcgccggtgc tgctcgcccc gatcggcgtg ctggagacgg cgcatcgcca
     5461 ggcggacctt gcggtggcac gcgcggccgc cgcgatcggc gtgcccttcg tgttctccag
     5521 ccaggcctcg gtcaacatgg agacgtgcgc ggccgcgatg ggcgacagcc cacggtggtt
     5581 ccagctctac tggagccggt ccaacgaggt ggtgaagagc ttcgtgcgcc gggcggaggc
     5641 ctgcgggtgt tcggccatcg tcctgacgct cgacaccacc ttgctgggat ggcggcctcg
     5701 cgatctcgac ctcggatcgc tgccgttcct gcgcggcatg ggcctcgccc agtacacgac
     5761 cgatcccgcc tttctcgcag agctgcacga gccgctcgac gtgccggtca tgcaaccgcc
     5821 gctgaccctc tccaccctcc gggtggcgct ggagcagaag aagcgctttc ccggcggtct
     5881 gctccgcaac ctgttctcgg acgagccgcg gctggccgtg cagcggttcg tggccaccta
     5941 ctcgcgcccg tcactacaat gggaggacct ggcgctgctg cgcagcttca cccggctgcc
     6001 cattctgctg aagggcgtgc tgcatcccga cgacgcgcga cgcgcggtcg aggcgggcat
     6061 ggacggcgtg atcgtctcca accacggcgg ccggcaggtg gacggtgcca tcgcggcgct
     6121 cgatgcgctc cggggcatcg tggatgcggt ccaggcgcgc gtgccggtgc tgttcgacag
     6181 cggcatccgc ggcggcgcgg acgtcttcaa ggcgctcgcg ctcggcgcgc gcgcggtgct
     6241 gctcgggcgg ccgtacgtgt acggcctggc cctggccggc gagcgtggtg tgagggatgt
     6301 gatcctgaac gttctcgccg agctcgatct caccatgggc ctcagcggct gccggaacct
     6361 gcaagaggtg acacgtgaga tgctggcgag agcctagtcc gcgccgttgc cgatcacggc
     6421 cctggaacat caccggatga atcgctggac aaatcgggtg cctggttggg aaaagctgtt
     6481 catcctccgt agatgccgtc accaccatcc gccgcggacc cgtatgcgcc ggccccggat
     6541 gcgcctgccg acccggcggc c
//
LOCUS       DASVOR010001636         4567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_572724,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001636 DASVOR010000000
VERSION     DASVOR010001636.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572724"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..280)
                     /locus_tag="VF516_30675"
     CDS             complement(<1..280)
                     /locus_tag="VF516_30675"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8112152.1"
                     /translation="MSRPHVVVIGGGFGGLAAARGLARAPVNVTLIDRRNHHLFQPLL
                     YQVATAALNPSDIAYPIRGALAHQHNARVLLAAATAIDTANRRVLLDDG"
     gene            complement(368..1501)
                     /locus_tag="VF516_30680"
     CDS             complement(368..1501)
                     /locus_tag="VF516_30680"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEX8112153.1"
                     /translation="MTTVTVPFVDVPALIRPSRAEYLAAFERLLDTGGFIGGPAVNEF
                     EAAFAAYCGAPYAIAVRTGTDALVLALRALGIGVGDEVITAANSFFATAEAISHAGAK
                     PVLADVDDATLLLDPADVARRVTRRTRAVIPVHLFGQVADMAAIAAVAEAHRLHVLED
                     SAQAHGATRRGPDGADHRAGGIAEIAAFSFYPTKNLGALGEGGAVTTQDGELAELVRR
                     LRDHGQAGRHDHVEIGYNARLDALQCACLSVSLRRLDAGNAARRRIAAHYRARLADLP
                     GVRLIAEPAGSAPVYHLMVIRVDAARRDAIRDRLAAAGIATAIHYPRPIHLQRAYAYL
                     GQAPGTCPVAERAADEMISLPMFPDLTIAQVDRVVDALGAALR"
     gene            complement(1498..2502)
                     /locus_tag="VF516_30685"
     CDS             complement(1498..2502)
                     /locus_tag="VF516_30685"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8112154.1"
                     /translation="MIGYGYWGPNLVRNFAAGPRTRVVAIAEASPARRQAAAVAVPHI
                     RCLAEAAELIRDPEVDAVAIATPIFTHHPLAKAALEAGKHVLVEKPLAPSVAQAEELA
                     QLAARAGRVLMVDHTFVYTGAVRKIAALIAAGELGRLLYVDSVRINLGLFQPDYNVIW
                     DLAPHDLTIIDFVLGKTAGATARWLSAIGVSHYGRHENLAYLTIGFDEGLLAHIHVNW
                     VAPVKTRRMIIAGSKKMLVWDDTSPVEPVKVYESSVDVSTIDKESAYALNVQYRSGDV
                     TSPKLDGKEALSVMAQAFAAACLDGKPSDSDAAIGVRIVRMLEAAQRSLEQQGARISL
                     "
     gene            complement(2590..>4567)
                     /locus_tag="VF516_30690"
     CDS             complement(2590..>4567)
                     /locus_tag="VF516_30690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112155.1"
                     /translation="DRVAGSARRLWAPGGRRPRLVLVAAFAVIVGMYCTNQDMGGQPD
                     APRGDGVYRPVLARGDGHMMYLMARSTALDGDWRFDNDLARFGDPWRQRTTRTGRKAI
                     AHPIGPALVWTPLIWIAQAGAVIANALGARIELHGYTLWHQRLVFLSSALAACGAALL
                     GARLARRLLGGAWSASYGAAAVLLGTSLTYYATYMPSYSHALDALACAGFLGYWAHSA
                     GRCDLRRWLVLGLLLGIAALIRVQELAMGVVVALEIAAETVKETVRGRGWHEVGRWWI
                     GGALAVAVTLVVFVPQLVEWHLVFGSITELPQGAKYTRLEAPMIGELLFAPRNGWFST
                     TPIAYAAVLGLLALPRRAALVAVGLVAAVALQVYLNSTILDWWGSAAFGQRRMCNATL
                     PMIVGLAALVWRAGRLARRLPVAARHAIAWAVLGPLVAWNLMRVGELRGGVSAPDAVL
                     PACCDRVPRPLRASFAWLYGRIGDPFELPASAWFAWWHGVPIDRWDRTVGNYPLVPGL
                     DTLLDDSLWAQRGVWRIGGGIDPYLVSGWSRAVRGSRPLRWTTAPVATVLVPNLMPYR
                     QRLTLWLAPAGAHHVTVRWNGAVVADTELGDWSGVWFELPDIGLHTNELSIEAVPAPF
                     TPATGPAPEGPVGVAVGDLEIELIDPRSEPRSRP"
BASE COUNT          649 a         1767 c         1602 g          549 t
ORIGIN      
        1 ctccgtcgtc gagcagcacc cgccggttcg cggtgtcgat cgcggtcgcg gcggcgagca
       61 gcacgcgcgc gttgtgctgg tgcgccagcg cgccgcggat cgggtacgcg atgtcgctcg
      121 ggttgagcgc cgcggtcgcc acctggtaga gcagcggctg gaacaggtgg tggttgcggc
      181 gatcgatcag cgtgacgttg accggcgcac gggcgagccc gcgggccgcg gccagccccc
      241 cgaaaccacc cccgatcacc acgacgtgcg gtctggacat acctgttaga tggggggatc
      301 ccccgcgggg atgcaaggcc gacaacgccg cgcaatgact gcgtccggcg ccgcgcgggc
      361 gccgccgtca gcgcagcgcc gcgccgaggg cgtcgaccac gcgatcgacc tgcgcgatcg
      421 tcagatccgg aaacatcggc agcgagatca tctcatccgc ggcgcgctcg gcgaccgggc
      481 aggtccccgg cgcctggccg aggtacgcgt aggcgcgctg gaggtggatc ggccgcgggt
      541 agtggatcgc ggtcgcgatg ccggccgcgg cgagccggtc gcggatcgcg tcgcggcgcg
      601 cggcatcgac ccggatcacc atcaggtgat agaccggcgc ggatcccgcc ggctcggcga
      661 tcagccgcac gcccggcagg tcggcgagcc gggcgcggta gtgcgccgcg atccggcggc
      721 gcgccgcgtt gccggcgtcg agccggcgca ggctcaccga caggcacgcg cactgcagcg
      781 catcgagccg cgcgttgtag ccgatctcga cgtgatcgtg ccgcccggcc tggccgtgat
      841 cgcgcagccg gcgcaccagc tcggcgagct cgccgtcctg cgtggtgacc gcgccgccct
      901 cgccgagcgc tccgaggttc ttggtcgggt agaagctgaa cgcggcgatc tcggcgatcc
      961 cgccggcgcg gtggtccgcc ccgtcgggcc cgcgccgggt cgcgccgtgc gcctgcgcgc
     1021 tgtcctcgag cacgtgcagc cggtgcgcct cggcgaccgc ggcgatcgcg gccatgtcgg
     1081 cgacctggcc gaacagatgg accgggatca cggcgcgggt ccgccgggtc acccggcgcg
     1141 cgacgtcggc cggatcgagc agcagcgtcg cgtcgtcgac gtcggccagc accggcttcg
     1201 cgccggcgtg gctgatcgcc tccgcggtgg cgaagaacga gttggccgcg gtgatcacct
     1261 cgtcgccgac gccgatcccg agcgcgcgca gcgcgagcac cagcgcgtcg gtcccggtgc
     1321 ggaccgcgat cgcgtacggc gcgccgcagt acgccgcgaa cgcggcctcg aactcgttga
     1381 ccgccgggcc gccgatgaac ccgccggtgt cgagcaggcg ctcgaacgcc gcgaggtact
     1441 ccgcccggct cgggcggatc agtgcgggga cgtcgacgaa gggtacggtc acggtggtca
     1501 tagtgagatc ctcgcgccct gctgctccag cgagcgctgc gcggcctcca gcatgcggac
     1561 gatgcgcacg ccgatcgcgg catcggagtc cgacggcttg ccgtcgaggc acgccgcggc
     1621 gaacgcctgc gccatcaccg atagcgcctc cttgccgtcg agcttgggcg aggtcacgtc
     1681 gcccgagcga tactgcacgt tgagcgcgta ggccgactcc ttgtcgatcg tcgacacgtc
     1741 gaccgagctc tcgtagacct tgaccggctc gaccggcgag gtgtcgtccc acaccagcat
     1801 cttcttgctg ccggcgatga tcatccgccg ggtcttgacc ggcgccaccc agttgacgtg
     1861 gatgtgggcg agcaggccct cgtcgaaccc gatcgtcagg tacgcgaggt tctcgtggcg
     1921 gccgtagtgc gacacgccga tcgccgacag ccagcgcgcg gtcgcgccgg cggtcttgcc
     1981 gagcacgaag tcgatgatcg tcagatcgtg cggcgcgagg tcccagatca cgttgtagtc
     2041 gggctggaac agcccgaggt tgatccgcac cgagtcgacg tacagcaggc ggccgagctc
     2101 gccggcggcg atcagcgcgg cgatcttgcg gaccgcgccg gtgtacacga acgtgtggtc
     2161 caccatcagc acgcggccgg cgcgcgccgc gagctgggcg agctcctcgg cctgggcaac
     2221 gctcggcgcg agcggcttct cgaccagcac gtgcttgccg gcctcgagcg ccgccttggc
     2281 cagcgggtgg tgggtgaaga tcggcgtcgc gatcgcgact gcgtcgacct cgggatcgcg
     2341 gatcagctcc gccgcctcgg cgaggcaccg gatgtgcggc acggccacgg ccgccgcctg
     2401 ccgccgcgcc ggcgacgcct cggcgatcgc cacgacccgg gtccgcgggc ccgccgcgaa
     2461 gttgcgcacc aggttcggtc cccagtagcc atagccgatc acgccgattc gaagcggaga
     2521 cgctgccaca ggcgggggac tctcacacgg ggctcccgcg cggtcaagcg acggcgctca
     2581 cggcggagat cagggccgcg agcgcggctc cgagcgcgga tcgatcagct cgatctccag
     2641 atcgccgacc gcgacgccga ccggcccctc gggcgccggc ccggtcgccg gcgtgaacgg
     2701 cgccggcacg gcctcgatcg agagctcgtt ggtgtgcagc ccgatgtccg gcagctcgaa
     2761 ccacaccccg ctccagtcgc cgagctcggt gtcggccacc accgcgccgt tccaccgcac
     2821 cgtcacgtgg tgcgccccgg ccggcgccag ccacagcgtc aaccgctgcc ggtacggcat
     2881 caggttgggc accagcaccg tcgcgaccgg cgccgtcgtc cagcgcagcg gccggctgcc
     2941 gcgcaccgcg cgcgaccacc cggacaccag gtacggatcg atcccgccgc cgatccgcca
     3001 cacgccgcgc tgcgcccaca gcgagtcatc gagcagcgtg tcgagccccg gcaccagcgg
     3061 atagttgccg accgtgcggt cccagcgatc gatcggcacg ccgtgccacc acgcgaacca
     3121 cgcgctcgcc ggcagctcga acggatcgcc gatccggccg tacagccacg cgaagctcgc
     3181 gcgcagcggg cgcggcacgc gatcgcagca cgccggcagc accgcgtcgg gcgcggacac
     3241 cccgccgcgc agctcgccga cccgcatcag gttccacgcc accagcggcc cgagcaccgc
     3301 ccacgcgatc gcatggcgcg ccgccaccgg caaccggcgc gcgagccgcc cggcgcgcca
     3361 cacgagcgcc gccagcccca cgatcatggg cagcgttgcg ttgcacatcc ggcgctggcc
     3421 gaacgccgcg ctgccccacc agtcgaggat cgtgctgttg aggtagacct gcagcgcgac
     3481 cgcggcgacg agcccgaccg cgaccagggc cgcccgccgc ggcagcgcga gcaggccgag
     3541 caccgccgcg tacgcgatcg gcgtggtcga gaaccagccg ttgcgcggcg cgaacaggag
     3601 ctcgccgatc atcggcgcct cgagccgcgt gtacttcgcg ccctgcggca gctccgtgat
     3661 gctgccgaac accaggtgcc actccaccag ctgtggcacg aacaccacca gcgtcaccgc
     3721 gaccgccagc gcgccgccga tccaccaccg gccgacctcg tgccagccgc gcccgcgcac
     3781 ggtctccttc acggtttccg cagcgatctc cagcgcgacg accacgccca tcgccagctc
     3841 ctgcacccgg atcagcgcgg cgatcccgag cagcagcccg agtaccagcc agcgccgcag
     3901 gtcgcatcgc ccggcgctgt gcgcccagta gccgaggaac cccgcgcacg ccagcgcatc
     3961 gagcgcgtgc gagtagctcg gcatgtacgt cgcgtagtag gtcagcgagg tcccgagcag
     4021 cacggccgcc gcgccgtagc tcgccgacca cgcgccgccg agcagccgcc gcgccagccg
     4081 ggcaccgagc agcgccgcgc cgcacgccgc cagcgcgctc gacaggaaca ccagccgctg
     4141 gtgccacagc gtgtagccgt gcagctcgat ccgcgcgccc agcgcgttcg cgatcaccgc
     4201 gccggcctgc gcgatccaga tcagcggcgt ccacaccagc gccggcccga tcgggtgcgc
     4261 gatcgccttg cgcccggtcc gcgtcgtgcg ctgccgccac ggatcgccga accgcgccag
     4321 gtcgttgtcg aaccgccagt cgccgtcgag cgcggtcgac cgcgccatca ggtacatcat
     4381 gtggccgtcg ccgcgcgcca gcaccggccg gtacaccccg tcgccgcgcg gcgcgtcggg
     4441 ctgcccgccc atgtcctggt tggtgcagta catccccacg atcaccgcga acgccgcgac
     4501 cagcaccagc cgcggccggc gcccgcccgg cgcccacagc cgccgcgccg atccggccac
     4561 gcgatcg
//
LOCUS       DASVOR010001637         1995 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_75085,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001637 DASVOR010000000
VERSION     DASVOR010001637.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75085"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /locus_tag="VF516_30695"
     CDS             complement(<1..1092)
                     /locus_tag="VF516_30695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112156.1"
                     /translation="MILKNLAAVRSRTTLAGIAISWLPLVGCVDKPDDVGQSEQAVGT
                     PSVTQTPLDPTTIPLYATQLPIPRVYAPTVITDSSGHVIRHEYTVSARLATVQMLPPG
                     FPATTALAYSGNVKIPGSTATEFVSTVPGPAFDNTRGIPALIHWQNQLTGPTFMPVDT
                     TLAWANPLGKEPPVGPFQKFPTVDLGGQSPVPIVTHNHGLVVVPQNDGIADEWFTAGS
                     ERGPNFTTQDYFEPNQQPGTQLFYHDHTLGITRLNVYAGLNGPAYFIRDPNNPLDQAS
                     SPLPKGEFEIPLSIASKSFFTDGEMNYPRSSPNIKNAYWAPADNSDTNIVNGVVWPNL
                     NVKRQQYRFRIVATASNAVFHISINQQPPI"
     gene            1601..>1995
                     /locus_tag="VF516_30700"
     CDS             1601..>1995
                     /locus_tag="VF516_30700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112157.1"
                     /translation="MRSDATRSPLRTVLVVGAVLVVAAGVVECGVLRRPVHEDTEPGR
                     EVASQRAPSPTERTPVARPALPQARPVEPAPGQPGQPGQPHFVGVGAAEANRKLTQIK
                     VQKYVHEAYPAWRQAHPGQECPHRLIELN"
BASE COUNT          331 a          599 c          697 g          368 t
ORIGIN      
        1 gatgggcggt tgctggttga tcgagatgtg gaatacggcg ttgctggcgg tggccacgat
       61 ccggaaccgg tactgctggc gcttgacgtt gaggttcggc cacaccacgc cgttgacgat
      121 gttggtgtcg gagttgtcag ccggcgccca gtacgcgttc ttgatgttgg ggctcgaccg
      181 cgggtagttc atctcgccat cggtgaagaa cgacttggag gcgatcgaga gcgggatctc
      241 gaactcgccc ttgggcagcg gcgagctcgc ctggtcgagc gggttgttgg gatcgcggat
      301 gaagtaggcc ggcccgttga gtccggcgta cacgttgagc cgcgtgatac ccagcgtgtg
      361 atcgtgatag aacagctgcg tgcccggctg ctggttgggc tcgaagtagt cctgggtcgt
      421 gaagttcggc ccgcgctcgg agcccgcggt gaaccactcg tcggcgatgc cgtcattctg
      481 cggcacgacg acgaggccgt ggttgtgggt cacgatcggg accggggact ggccaccgag
      541 gtcgacggtc gggaacttct ggaacggccc gaccggcggc tccttcccga gcgggttcgc
      601 ccaggcgagc gtggtgtcga ccggcatgaa ggtcggcccg gtgagctggt tctgccagtg
      661 aatgagggcg gggatgccac gggtgttgtc gaacgccggg cccggcaccg tgctcacgaa
      721 ctcggtcgcc gtcgaacccg ggatcttcac attgccgctg tacgccaggg cggtggtggc
      781 cgggaaaccc ggcggcaaca tctgtaccgt tgccagccgc gcgctcacgg tgtactcgtg
      841 gcggatgacg tgtcccgaag aatcggtgat cacggtcggg gcgtagaccc gcgggatcgg
      901 cagctgggtc gcgtacaggg gaatcgttgt cgggtcgagt ggcgtctggg tcaccgaggg
      961 cgtgcccaca gcttgctcgc tctggccgac gtcgtctggt ttgtcgacac agccgaccag
     1021 cggcagccat gaaatagcga tcccggccag ggtggttctc gatctaactg cagcgagatt
     1081 cttcaatatc atcctcatcc tcctgcgcgt ggatgacgtt tccccggccc ggatgagctc
     1141 cgggtgatcc aggtgtttgt tggagcaccg taggattttt tcgcccacgt catcaagtcc
     1201 aggaatccag gacaccgcgc tttgcacatc ctagaacgct gcattctgga acacagcttc
     1261 gaaaaactga gaactgccga agtccgcgat atgcgatctg gaatcgccag atgccgtcag
     1321 ggtttggaga tctgcgtcct tcccgatgaa gtccgcgatc gggatcatcg ctgtcgcgat
     1381 ccggcgtcgg tgacgcggac agtcggtaca cagactgcaa tactcccatt ggtgaacgcc
     1441 atatagtgtg cgcaatgaag gatcgtcaca caatgcagat cgattcaact caccggggcg
     1501 ccgagctcac gcaccgcgtt accgtgagac cgggcgggtc ggctgtgcgg gcccgctgtc
     1561 tgcgatggat tgtgccgtcg cgccgacata cgattccgcg atgagatcgg atgccacgag
     1621 aagtcccctg cgcaccgtgc tcgttgtcgg cgctgtcctg gtggtggcgg ccggggtcgt
     1681 cgagtgcggc gtgcttcgtc gtcccgtgca cgaggacaca gagcccgggc gcgaggtcgc
     1741 cagccagcgc gctccctctc cgacggagcg aacgcccgtg gcgcgaccgg cgttgccgca
     1801 ggcaaggccc gtcgagccgg cgccgggaca gccggggcag cccgggcagc cgcacttcgt
     1861 gggcgtcggc gcggccgagg ccaaccgcaa gctcacccag atcaaggtgc agaagtacgt
     1921 ccacgaggcc tacccggcgt ggcgccaggc gcaccccggc caggagtgtc cgcaccggct
     1981 gatcgagctc aacga
//
LOCUS       DASVOR010001638         3825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_75089,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001638 DASVOR010000000
VERSION     DASVOR010001638.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75089"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VF516_30705"
     CDS             <1..369
                     /locus_tag="VF516_30705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112158.1"
                     /translation="RARSTALVPLPQIRVRLAGRERLLLLDPFVVLAGAALPPLAAGP
                     EIDLAFWLPAAALWDLGNATCHVLHDGETLVYPAIRTPHGVIFGITLRVLTLLSDQLG
                     VPLPMIEEIPQLRRAPGAPR"
     gene            437..1603
                     /locus_tag="VF516_30710"
     CDS             437..1603
                     /locus_tag="VF516_30710"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX8112159.1"
                     /translation="MADQDEDFAAMLAESERSAPRAKKRPRVGDQITGKVISIGKDAV
                     FVDVGGKAEGQLERGEVSDPEGKLLVKLGDPIEARVIGDFGGVLTLRVKLGRGPEARA
                     ELQQAMELGIPVEGVVTEVVKGGVAVDVAGVRGFCPASQLDVRFIDDLSTYIGQRLTF
                     RITRYEPRNLVLSRRALLEEDNARRAAETRKKLEPGAVLRGKVVGFKPFGAFVDLGGI
                     EGMLHISELGYARVERPEDALSLGQELDVAVLKIEPAAKGDKGERISLSLKALATDPW
                     RDAAASLVEGARLRGKITRLSQFGAFVEIAPGVEGLVHISELGAGRRINHPKEVVSVG
                     QDVEAVVLSIDHERRRIGLSMAASAGGTDADVAAVTKAHAPTRLGTFGDLLKKR"
     gene            1647..1832
                     /locus_tag="VF516_30715"
     CDS             1647..1832
                     /locus_tag="VF516_30715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112160.1"
                     /translation="MRKFYLLLLVCAACAKKPAAKQPAPPPPAEEQGKQPDSAPKPEP
                     TATPRIGDPCDGGQKPQ"
     gene            complement(2122..2826)
                     /gene="rhuM"
                     /locus_tag="VF516_30720"
     CDS             complement(2122..2826)
                     /gene="rhuM"
                     /locus_tag="VF516_30720"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HEX8112161.1"
                     /translation="MRVAGLAMPRRRAAEARRRRELLRRAARTDPRHPFLREDVLAQG
                     AGHLRDQHRLRSARRGFPAVLRDGAEQDALGGARHTAAEIIAGRADATRPNMGMTSWS
                     GDMPRKADALVAKNYLGADEIEALNRIVTAYLEFAELQAIDRKPMYMADWIAKLDDFL
                     KLSDREILNHAGRISHEAAVAKAELEYDNFAAARAALPAPAERHFEQVTREVKQLEKA
                     QRTARASTTGRARKKP"
     gene            3030..>3825
                     /locus_tag="VF516_30725"
     CDS             3030..>3825
                     /locus_tag="VF516_30725"
                     /inference="COORDINATES: protein motif:HMM:NF019619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8112162.1"
                     /translation="MDLAELLAPIAITELVRDYWQRRPLYIPGAPDKLAGLFDRAAFD
                     RAVTAIEPDELGRRPFAKAGAQDAAGDHVELRIQPEQIPALVRAGMTIQAEQLETAHA
                     GLRALLDDTRRQLDVAAAMDVGAFLSPDGAGYGLHFDATGMFTLQIAGSKRWGYGRAP
                     AVAWPRANRVATRGARDQLPDDELDEVVLRPGDVLYLPPGTWHRASAIGESLHVSLTV
                     RPANLVERVAELLEPLLIDPRWRRLPEPGDRLSDRLRELVAVVTDLT"
BASE COUNT          551 a         1418 c         1263 g          593 t
ORIGIN      
        1 cgagcgcggt cgaccgcgct cgtgccgctc ccccagatcc gcgtccgcct cgccggtcgc
       61 gagcgcctgc tgctgctcga cccgttcgtc gtgctcgccg gcgccgcgct gccgccgctc
      121 gccgccggtc cggagatcga cctcgcgttc tggctgccgg ccgccgcgct gtgggacctc
      181 ggcaacgcca cctgccacgt cctccacgac ggcgagaccc tggtctatcc cgcgatccgc
      241 accccgcacg gcgtgatctt cggcatcacc ctgcgcgtgc tcacgctgct gtccgaccag
      301 ctcggcgtcc cactccccat gatcgaggag atcccacagt tgcgccgcgc ccccggcgcc
      361 ccgcgctgac cggcgtgtcc ggctttgccg gccgcgcgct ggcctccccg gcatttgccg
      421 cgtagagtgc gcgccaatgg ccgatcaaga cgaagacttt gcagccatgc tcgccgagag
      481 cgagcgcagc gccccccgcg cgaagaagcg cccgcgggtc ggtgaccaga tcaccggcaa
      541 ggtgatctcg atcggcaagg acgcggtgtt cgtcgacgtc ggcggcaagg ccgagggcca
      601 gctcgagcgc ggcgaggtca gcgaccccga gggcaagctc ctggtcaagc tcggcgaccc
      661 catcgaggcc cgcgtgatcg gcgacttcgg cggcgtgctc accctgcgcg tcaagctcgg
      721 ccgcggcccc gaggcgcgcg ccgagctcca gcaggccatg gagctcggca tcccggtcga
      781 gggcgtcgtc accgaggtcg tcaagggcgg cgtcgcggtc gacgtcgccg gcgtccgcgg
      841 gttctgcccc gcgtcccagc tcgacgtccg gttcatcgac gacctctcca cgtacatcgg
      901 ccagcgcctg acgttccgca tcacccgcta cgagccgcgc aacctcgtgc tgtcgcgccg
      961 cgcgctgctc gaggaggaca acgcccggcg cgccgccgag acccgcaaga agctcgagcc
     1021 cggcgccgtg ctgcgcggca aggtcgtcgg cttcaagccg ttcggcgcgt tcgtcgacct
     1081 cggcggcatc gagggcatgc tgcacatctc cgagctcggc tacgcccgcg tcgagcgccc
     1141 cgaggacgcg ctcagcctcg gccaggagct cgacgtcgcc gtcctcaaga tcgagcccgc
     1201 cgccaagggc gacaagggcg agcggatcag cctgtcgctc aaggcgctcg ccaccgatcc
     1261 gtggcgcgac gccgcggcct cgctcgtcga gggtgcccgc ctccgcggca agatcacccg
     1321 gctctcgcag ttcggcgcgt tcgtcgagat cgcccccggc gtcgagggcc tggtccacat
     1381 cagcgagctc ggcgccggcc gccgcatcaa ccaccccaag gaggtcgtgt cggtcggcca
     1441 ggacgtcgag gccgtcgtgc tgtcgatcga ccacgagcgc cgccggatcg gcctgtcgat
     1501 ggccgcctcg gccggcggca ccgacgccga cgtcgccgcg gtgaccaagg cccacgcgcc
     1561 gacccggctc ggcaccttcg gcgacctgct caagaagcgc tgaccggatc gaccacgagg
     1621 cagcgacatc cgctaggatg gtcgcgatga ggaaattcta tcttctcctg ctggtgtgtg
     1681 cggcgtgtgc gaagaagccc gcggccaagc aacctgctcc tccgccgccc gccgaagagc
     1741 agggcaagca accggactct gcgcccaagc cggagcccac cgccacccca cgcatcggcg
     1801 acccctgcga cggcggccag aaaccgcagt gacgaccact ccctgggtcg gctgacgacg
     1861 tatcgcggtt gggtgtccac gagtcgtgct gaaatcgtgc acctccggga atcggtgcaa
     1921 ccaacacgag cccacgctac gcgtactctc tccgatatga acgggagccc gatatgaaca
     1981 ggaacacatt ccacagtagt ttcgtcgttc tctcgcggag ctgagaagtc tgggccttac
     2041 taccctggcg ctgcgttgct ggtgcaacaa cctcgactca gatgacgccg acgtatccag
     2101 gcgcaatgca aaggcactga gttacggctt cttgcgggcc ctgcctgtcg tcgacgccct
     2161 cgccgtccgc tgggctttct cgagctgctt cacctctcgt gtgacctgct cgaagtggcg
     2221 ctcggccgga gcgggaagcg cagcgcgcgc ggccgcaaag ttgtcgtact ccagttcggc
     2281 cttggccacg gccgcctcgt gcgagatccg accagcgtgg ttcaggatct cgcggtcgct
     2341 caatttgagg aaatcatcga gcttcgcgat ccagtcggcc atgtacatcg gcttgcgatc
     2401 gatcgcctgg agctctgcga actccaggta ggccgtgacg atccggttga gcgcttcgat
     2461 ctcgtcggcc ccgaggtagt tcttcgccac caatgcgtcc gccttgcgcg gcatgtcgcc
     2521 cgaccagctg gtcatcccca tgttgggcct ggtcgcatcg gcccgccctg cgatgatctc
     2581 cgccgcggtg tgccgtgcgc cgcccagtgc atcttgttct gcaccgtcgc gaagaaccgc
     2641 tgggaagcct cgacgcgcgg atcgtagtcg atgctggtcg cgtaaatgtc cagcaccttg
     2701 cgccagaaca tcttctcgga ggaacggatg tcgcggatcc gtgcgagcag ctcgtcgaag
     2761 tagttcccgc cgccggcgcg cttcagccgc tcgtcgtcga ggcatggcga gcccggcgac
     2821 gcgcatcacg ccatcgaggg gtgtggtgcg ctcggcgatc gagggcgatc ccgatcggct
     2881 gcgcgatccg aaatggatcg agcaggtcgt ccgggtgacc gtgcggacga tcgcggaatc
     2941 gccggcgcag gcacgcgatg gccatacgcc acccggtgca tcttgttctg cacggtcttc
     3001 cttcccgcgc gctgcggtag agtcgctccg tggacctcgc ggagctgctc gcgccgatcg
     3061 ccatcaccga gctcgtccgc gactactggc agcggcgccc gctgtacatc cccggtgcac
     3121 cggacaagct ggcaggcctg ttcgaccgcg ccgcgttcga ccgcgcggtg accgcgatcg
     3181 agcccgacga gctcggccgg cgcccgttcg ccaaggctgg agcgcaggac gctgcgggcg
     3241 accacgtcga gctccggatc cagcccgagc agatcccggc gctggtgcgc gctggcatga
     3301 cgatccaggc cgagcagctc gagacggcgc acgccggcct gcgcgcgctg ctcgacgaca
     3361 cccggcgaca gctcgacgtc gccgccgcga tggacgtcgg cgcgttcctg tcccccgacg
     3421 gggccggcta tggcctgcac ttcgacgcga ccggcatgtt cacgctgcag atcgccgggt
     3481 ccaagcgctg ggggtacggc cgcgctcccg ccgtggcctg gccgcgcgcc aatcgcgtcg
     3541 cgacccgggg ggcgcgcgac cagctgccgg acgacgagct cgacgaggtc gtgcttcgcc
     3601 ccggtgacgt cctgtacctg ccaccgggca cgtggcatcg cgcgtcggcg atcggcgagt
     3661 cgctccacgt ctcgctgacc gtccgtccgg cgaacctggt cgagcgcgtc gccgagctgc
     3721 tcgaaccgct gctgatcgac ccgcgctggc ggcggctgcc cgagcccggc gatcggctat
     3781 ccgatcggct gcgcgagctg gtcgcggtcg tcaccgacct cacac
//
LOCUS       DASVOR010001639         1686 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_278956,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001639 DASVOR010000000
VERSION     DASVOR010001639.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_278956"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..876
                     /locus_tag="VF516_30730"
     CDS             <1..876
                     /locus_tag="VF516_30730"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112163.1"
                     /translation="EALDRTATAHGVAKPSLTTTLEPGQVLAQRYRVQRLLGKGGMGA
                     VYLADDEVLGELVALKVILSAFAADEAAMIARFRREAAAARKVSSSSVIRIHDLGEAR
                     PGLLYLSMEYFAGRTLTEVISQRGVVPLKDAQDMLQQIAAGLEAAHQAGVIHRDLKPS
                     NVLVGERGMVKIIDFGLATTAVGEGLTATGAILGTPHYMAPEQVRGKPVDARTDIYAL
                     GALAYHLVCGKPPFAGDNPIAIGFAHISEPVVPPRQLRKEVSPALEAAILAALAKAPE
                     DRPPSARAFIEKAFS"
     gene            complement(869..>1686)
                     /locus_tag="VF516_30735"
     CDS             complement(869..>1686)
                     /locus_tag="VF516_30735"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8112164.1"
                     /translation="HLKYSCAYWPAETEAETGVAALGAAEAAMLELTAARAGLADGQA
                     VLDLGCGWGALTLWAAARFPRSRFTAISNSRSQRDYIAEQACRRGLSNATVRVADVRT
                     LELPAGAFDRVVSIEMFEHMRNYQVLLHRIAGWLAPGGALFVHVFAHRRHAYPFEDAG
                     ASDWMAREFFTGGLMPSVDLLRDFQDDLAITADWWLDGGHYARTAEAWYGNLMAHRAE
                     AAELLDPGPRGRARVERWRVFFLACAELFGFRGGREWGVAHYRFERSGRARAS"
BASE COUNT          242 a          620 c          617 g          207 t
ORIGIN      
        1 gaggcgctcg accgcaccgc gaccgcacac ggcgtcgcca agccttcgct gacgacgacg
       61 ctcgagcccg gtcaggtgct cgcgcagcgc tatcgcgtcc agcgcctgct cggcaagggc
      121 gggatgggcg cggtgtacct cgccgacgac gaggtgctcg gcgagctggt cgcgctcaag
      181 gtgatcctga gcgcgttcgc ggcggacgaa gccgcgatga tcgcgcggtt ccgccgcgag
      241 gccgcggcgg cgcgcaaggt gtcgtcgtcg tcggtgatcc ggatccacga cctcggcgag
      301 gcgcggccgg gcctgctcta cctgtcgatg gagtacttcg ccggccgcac gctgaccgag
      361 gtgatctccc agcgcggcgt cgtgccgctc aaggacgcgc aggacatgct gcagcagatc
      421 gccgccgggc tggaggccgc ccaccaggcc ggcgtgatcc accgcgatct caagccgagc
      481 aacgtcctgg tcggcgagcg cggcatggtc aagatcatcg acttcggcct ggcgaccacc
      541 gcggtcggcg aggggctgac cgcgaccggc gcgatcctcg gcacgccgca ctacatggcg
      601 cccgagcagg tccgcggcaa gccggtcgac gcccgcaccg acatctacgc gctcggcgcg
      661 ctcgcctatc acctggtgtg cggcaagccg ccgttcgccg gcgacaaccc gatcgcgatc
      721 ggcttcgcgc acatctccga gccggtggtg ccgccgcgcc agctgcgcaa ggaggtgtcg
      781 ccggcgctcg aggcggcgat cctcgccgcg ctcgccaagg cgcccgagga ccggccgccg
      841 agcgccaggg cgttcatcga gaaggcgttc agctagcgcg ggcgcggccg cttcgctcga
      901 accggtagtg ggcgacgccc cactcgcggc cgccgcggaa cccgaacagc tcggcgcacg
      961 ccaggaagaa cacccgccag cgctcgacgc gcgcccggcc ccgggggcca ggatcgagca
     1021 gctcggccgc ctccgcgcgg tgcgccatca ggtttccgta ccaggcctcg gcggtgcgcg
     1081 cgtagtggcc gccgtcgagc caccagtcgg cggtgatcgc gaggtcgtcc tggaagtcgc
     1141 gcagcaggtc gaccgacggc atcagcccgc cggtgaagaa ctcgcgcgcc atccagtccg
     1201 acgcgccggc gtcctcgaac gggtaggcgt gccggcggtg cgcgaacacg tggacgaaca
     1261 gcgcgccgcc gggcgcgagc cagccggcga tccggtgcag caggacctgg tagttgcgca
     1321 tgtgctcgaa catctcgatc gacaccacgc gatcgaacgc gccggccggc agctcgaggg
     1381 tccggacgtc ggcgacccgc acggttgcgt tggacaggcc gcgccggcac gcttgctcgg
     1441 cgatgtagtc gcgctgcgag cgcgagttcg agatcgcggt gaaccggctg cgcgggaagc
     1501 gcgcggcggc ccacagcgtc agcgcgcccc agccgcagcc caggtcgagc accgcctggc
     1561 cgtcggccag cccggcgcgg gcggcggtga gctccagcat cgcggcctcg gccgcgccga
     1621 gcgcagcgac tccggtctcg gcctcggtct cggccggcca gtaggcgcac gagtacttga
     1681 ggtgca
//
LOCUS       DASVOR010001640         1624 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_75098,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001640 DASVOR010000000
VERSION     DASVOR010001640.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1624
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75098"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VF516_30740"
     CDS             <1..1083
                     /locus_tag="VF516_30740"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:NF004679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEX8112165.1"
                     /translation="RMMAVAARQMGYRLLVLDPSPRCPTAQVADGVVVGALDDVEAAR
                     QLARQVDVITLDTEHVPADVLDALEPLAPVRPGASVLRTIQDRLVQKQFLDRIGLPQA
                     KWAPVDDPGMLRPALARVGRPAILKVRRAGYDGKGQIRIDPDGDAEALLGKLRGEPAV
                     AEQVIPFSREISVILARGLDGQIRIYPIAENVHRRHILHTTRAPAPMPEPRRRRAEEM
                     AVAVAEALGHVGVMAVEMFELPDGSLLVNEIAPRTHNSGHYTYGACATSQFEQHLRAV
                     CGVPLGDPRALTGAVMVNLIGDLWAQGAPPWHEVLAHPEARLHLYGKDGPAPGRKMGH
                     VVLLDDDTDRGLATAEALIATLAARS"
     gene            1103..>1624
                     /locus_tag="VF516_30745"
     CDS             1103..>1624
                     /locus_tag="VF516_30745"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8112166.1"
                     /translation="MATLIYSDVDGVDRSFALSNEPVTVGRSAECGIRSDDPRVSRLH
                     ARFFVEQGALWVEDLGSSNGIFVGPNRVQRAPVPTGEIILVGSLLIRLLPASGTLPPP
                     IGLHGTLASWLDMERKARAAVEDERDAFARRVGEMHNQLVSVMSARTLDPDAGSGVAE
                     LAHAREAAEARADA"
BASE COUNT          215 a          608 c          586 g          215 t
ORIGIN      
        1 cggatgatgg cggtcgcggc gcgccagatg ggctatcgcc tcctcgtgct cgatcccagc
       61 ccgcgctgcc cgaccgcgca ggtcgccgat ggcgtcgtgg tcggcgcgct cgacgacgtc
      121 gaggccgccc gccagctcgc ccgccaggtc gacgtgatca cgctcgacac cgagcacgtg
      181 ccggccgacg tgctcgacgc gctggagccg ctcgcgccgg tccgcccggg cgcatccgtg
      241 ctgcgcacga tccaggatcg cctggtccag aagcagttcc tcgaccgcat cggcctgccg
      301 caggcgaagt gggccccggt cgatgacccc gggatgctgc gcccggcgct cgcccgggtc
      361 ggccgcccgg cgatcctcaa ggtccggcgc gccggctacg acggcaaggg ccagatccgg
      421 atcgatccgg acggcgacgc cgaggcgctg ctgggcaagc tgcgcggcga gcccgcggtg
      481 gccgagcagg tgatcccgtt cagccgcgag atctcggtga tcctggcgcg cgggctcgac
      541 ggccagatcc ggatctaccc gatcgccgag aacgtgcacc gccgccacat cctgcacacc
      601 acgcgcgccc ccgcgccgat gccggagccc cgccggcggc gcgccgagga gatggccgtc
      661 gcggtcgccg aggcgctcgg ccacgtcggg gtcatggcgg tcgagatgtt cgagctcccc
      721 gacggcagcc tgctggtcaa cgagatcgcg ccgcgcaccc acaacagcgg ccactacacg
      781 tatggcgcgt gtgccacgtc gcagttcgag cagcacctcc gcgcggtgtg cggggtgccg
      841 ctcggcgatc cgcgcgcgct gaccggcgcg gtgatggtca acctgatcgg cgacctgtgg
      901 gcccagggcg cgccgccctg gcacgaggtc ctggcccacc ccgaggcccg gctccacctc
      961 tatggcaagg atggcccggc gcccggccgc aagatgggcc acgtggtcct gctcgacgac
     1021 gacaccgacc gcggcctggc caccgccgag gcgctgatcg cgaccttggc cgcgcgctcg
     1081 tgataacttc gggccctcgg ggatggccac tctgatctat tcggacgtcg atggcgtcga
     1141 tcggtcattt gcgctgagca acgagccggt cacggtcggg cgcagcgcgg agtgcgggat
     1201 ccgcagcgac gatccgcggg tgtcgcggct gcatgcccgg ttcttcgtcg aacagggcgc
     1261 gctgtgggtc gaggacctcg gcagctcgaa cgggatcttc gtcggcccca accgggtcca
     1321 gcgcgcaccg gtcccgaccg gcgagatcat cctggtcggc agcctgttga tccgcctgtt
     1381 gcctgcgagc ggcacgctgc cgccgccgat cggcctgcac ggcaccctgg cgtcgtggct
     1441 cgacatggag cgcaaggcgc gcgccgccgt cgaggacgag cgcgacgcgt tcgcccgccg
     1501 ggtcggcgag atgcacaacc agctggtcag cgtcatgtcg gcgcgcacgc tcgatcccga
     1561 cgccggcagc ggcgtcgccg agctcgcgca cgcccgggag gccgccgagg cccgcgcgga
     1621 cgcg
//
LOCUS       DASVOR010001641         1581 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_454978,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001641 DASVOR010000000
VERSION     DASVOR010001641.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1581
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454978"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VF516_30750"
     CDS             complement(<1..793)
                     /locus_tag="VF516_30750"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112167.1"
                     /translation="MAFDGPLIGGRYRLGPRLGKGSQGEIHIARDDKAKSSDDREVIV
                     KRLTPRGTWKSFELFERESKVLSQLRHAGVPRHIATIEEPPGTFNLVMQRAPGDNLRD
                     LAARRRLSQLELRDVLIRCLEILDYLHTRSPAVVHRDLKPSNIMRAPDGKIALVDFGG
                     VLDAARERGGSTIVGTFGYMAPEQLHGQATPATDIYALGATIVALAGGIEPEDVPRKG
                     LRMDLDRHLPSLDPGLRAALAAMTAPDPDRRPQRARDVFSLLAKGR"
     gene            complement(828..>1581)
                     /locus_tag="VF516_30755"
     CDS             complement(828..>1581)
                     /locus_tag="VF516_30755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112168.1"
                     /translation="PVRDPQSGLLHDAGGGSHRSEQGGFDAIVHPDGTVTLHDNPAFA
                     IHLAVPSLGDLGRAAASWYTCDKGASGKEGDPAMAGQIQVSPGATVAASDPIGTAPRD
                     HATTVIVPVLGGSFEVTDWLMRRHGQDPYAARKLRVLDATRDERVAIGKRHRAAELAR
                     TPEVVHRQLEVLWAQAADLAERKQGLFELWDDCVEIGDPELVAAAEAARRMILGFIRA
                     HLPAGSADAYTPAELAALARTRRSQTAFTPYD"
BASE COUNT          194 a          555 c          608 g          224 t
ORIGIN      
        1 gccggccctt ggcgagcagg gagaacacgt cgcgggcgcg ctgcgggcgg cgatccggat
       61 ccggagcggt catcgccgcg agcgcggcgc gcaggccggg atcgagcgac ggcaggtggc
      121 ggtcgaggtc catgcgcagg cccttgcgcg ggacgtcctc gggctcgatg ccgccggcga
      181 gcgcgacgat ggtcgcgccg agcgcgtaga tgtcggtcgc cggggtcgcc tggccgtgca
      241 gttgctcggg cgccatgtag ccgaacgtgc cgacgatcgt cgagccgccg cgctcgcggg
      301 ccgcgtcgag cacgccgccg aagtcgacca gcgcgatctt gccgtcgggg gcgcgcatga
      361 tgttcgacgg cttgaggtcg cggtgcacga ccgccggcga ccgggtgtgc aggtagtcga
      421 ggatctccag gcagcggatc agcacgtcgc gcagctcgag ctgggacagc cggcggcgcg
      481 ccgcgaggtc gcggaggttg tcgcccggcg cgcgctgcat gaccaggttg aacgtccccg
      541 gcggctcctc gatcgtcgcg atgtggcgcg gcacgccggc gtggcgcagc tgcgacagca
      601 ccttggactc gcgctcgaac agctcgaacg acttccaggt gccgcgcggt gtgaggcgct
      661 tgacgatgac ctcgcggtcg tcgctgctct tcgccttgtc gtcgcgcgcg atgtggatct
      721 cgccctgcga gcccttgccc aggcgcggcc cgagccgata ccgaccaccg atcagcgggc
      781 catcaaaggc catgtctctt gatagcacct cgccgcccgg tgcgaggcta gtcgtagggg
      841 gtaaacgcgg tctgcgagcg ccgggtccgc gccagcgcgg cgagctccgc cggcgtgtac
      901 gcgtctgcac tgccggccgg caggtgggcg cggatgaagc cgaggatcat ccggcgcgcg
      961 gcctcggcgg ccgccaccag ctccgggtcg ccgatctcga cgcagtcgtc ccacagctcg
     1021 aacagcccct gcttgcgctc ggcgaggtcg gccgcctgcg cccacagcac ctcgagctgg
     1081 cgatgcacga cctccggcgt ccgggcgagc tcggcggcgc gatggcgctt gccgatcgcg
     1141 acccgctcgt cgcgcgtggc gtcgagcacc ctgagcttgc gggccgcgta cgggtcctgg
     1201 ccgtggcggc gcatcagcca gtcggtgacc tcgaagctgc cgccgagcac cgggacgatc
     1261 accgtcgtgg cgtgatcgcg cggggccgtg ccgatcggat cggacgcggc gaccgtggcg
     1321 cccgggctga cctggatctg ccccgccatc gcggggtcgc cctccttgcc ggacgcgccc
     1381 ttgtcgcagg tgtaccagct cgcagcggcg cggccgaggt cgccgagcga cggcacggcg
     1441 aggtgaatgg cgaacgcggg gttgtcgtgc agcgtcaccg tgccgtcggg gtggacgatc
     1501 gcgtcgaacc cgccttgctc ggagcggtgg ctgccgccgc cggcgtcgtg gagcaggccg
     1561 ctctgcgggt cgcgcaccgg c
//
LOCUS       DASVOR010001642         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_425275,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001642 DASVOR010000000
VERSION     DASVOR010001642.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425275"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..546)
                     /locus_tag="VF516_30760"
     CDS             complement(130..546)
                     /locus_tag="VF516_30760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112169.1"
                     /translation="MRLHCALIGLVLAPAACSSSSSSTTLPGDGGGVLSDGGGSLTCG
                     STVAAYCGASGCQLTLAAAKQDTMLCPASIVSCADWDLIFRTAVDTSTTYYYFQGNLM
                     AVSHSVLPGKMSCLAGPSTFTPPSCQGTAQPLPACP"
     gene            complement(638..2350)
                     /gene="argS"
                     /locus_tag="VF516_30765"
     CDS             complement(638..2350)
                     /gene="argS"
                     /locus_tag="VF516_30765"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEX8112170.1"
                     /translation="MATHHTPLHRAAAEAVAAHAGIAATDLKVEAPPRPELGDLAVGC
                     FAIAKARQQNPAEVARAIAAAFQPTDLLESATAAGPFVNFRARRDTTYRWLIDSAQHG
                     RLVPGELGTDKTICIDYGSPNISKHLAYHHIRGTTIGHALAQIYRALGYRVIGINFLG
                     DWGTTHGMLLAAWAKWGPVEPLDVTALNALYVRFNAEKKMDPALEAEGRAWFKRLEDG
                     DPEARALWQRFRDVSWAEFRRVYDILGIEYDEVRGESAYEPDLPPLIERLAAQGLTTI
                     SQGALVVELADEKTPVLLRTRDGTTLYATRDVAAAEYRWANYHFTRSLYVVDRGQSLH
                     FRQLFRLLGRMGHEWATRCEHVPYGLVKINNQKSSSRLGTVLMHEVFTIAEDMVKSVI
                     AENNPEMPPARVAEVARQVGIGAIVFANLAPQRDKDVDFDIDNAVSLDGDSGPYLQYS
                     HARCASIARKAGGATPDTASIDPGRLTQDAEWAIARRLLEFPDAVVRAADACEPHVIA
                     HYLLGLAADFSRWYTLGNGDASLRVLCDAAATRQARLALVSAVQATLRDGLGLLGIAA
                     PDQM"
     gene            2517..>2937
                     /locus_tag="VF516_30770"
     CDS             2517..>2937
                     /locus_tag="VF516_30770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112171.1"
                     /translation="MSKIVLCVDDSATMQQVADITFRGTEFTYVGARGFDEGLDKARA
                     QKPIVVLADSHMPGKSGYDLCLALKTDPATSNIPVAILVGNAAPYDAARGVQVGADAN
                     LPKPWDTQTMLEKVAEVVTKAASGVAKPGQAAAAAPAP"
BASE COUNT          442 a          998 c         1076 g          421 t
ORIGIN      
        1 ggacgattcc tcgtcggcgg tcctcggaga aacggcgagg cgagtgggcg ggggattcac
       61 actccagctg tgccgccgtc cgcgcgccac ggaacacttc gacgtatcac tgcatgtcgc
      121 aggcggcgat cacgggcacg cgggcagggg ctgggccgtc ccctggcagc tcggcggcgt
      181 gaacgtcgac gggccagcga ggcagctcat cttgccgggc aggaccgagt ggctgaccgc
      241 catcaggttg ccctggaagt agtagtaggt ggtcgaggtg tccacggcgg tcctgaagat
      301 caggtcccag tcggcgcatg acacgatcga ggccgggcac agcatcgtgt cctgcttggc
      361 ggcggcgagg gtcagctggc agccgctcgc gccgcagtag gcagccaccg tcgagccgca
      421 ggtcaacgaa ccgccgccat ccgacagcac gccgccgccg tcgccgggca gcgtcgtgct
      481 cgacgagctc gacgagcagg ctgccggtgc gagcacgaga ccgatcaacg cgcaatggag
      541 tcgcatgggc acaatctacc cggcgagccg cgggaggtgt acgcggtggc cgcgggcgcg
      601 cacgcacagg gccgcgcggc cggctgtgca agccgcttca catctggtcg ggcgccgcga
      661 tgccgagcaa gcccaggccg tcgcgcaagg tcgcctgcac cgccgagacc agcgcgagcc
      721 gggcctggcg agtcgcggcc gcgtcgcaca gcacgcgcag cgacgcgtcg ccgttgccca
      781 gcgtgtacca gcgcgagaag tcggcggcga ggccgagcag gtagtgcgcg atcacgtgcg
      841 gctcgcaggc gtccgccgcg cggaccacgg catcggggaa ctccagcagc cggcgcgcga
      901 tcgcccactc ggcgtcctgg gtgaggcggc cgggatcgat gctggcggta tcgggggtgg
      961 cgccgccggc cttgcgcgcg atcgacgcgc agcgcgcgtg gctgtactgg aggtacgggc
     1021 cggagtcgcc atcgagcgac accgcgttgt cgatgtcgaa gtcgacgtcc ttgtcccgct
     1081 gcggggcgag gttggcgaac acgatcgcgc cgatcccgac ctggcgcgcg acctcggcga
     1141 cccgcgcggg cggcatctcg gggttgttct cggcgatcac gctcttgacc atgtcctcgg
     1201 cgatcgtgaa cacctcgtgc atcagcacgg tgccgagccg gctcgacgac ttctgattgt
     1261 tgatcttgac caggccgtac gggacgtgct cgcagcgcgt ggcccactcg tggcccatcc
     1321 ggccgagcag cctgaacagc tggcggaagt gcagcgactg gccgcggtcg acgacgtaga
     1381 gcgagcgcgt gaagtggtaa ttcgcccagc ggtactcggc ggccgcgacg tcgcgggtcg
     1441 cgtacagcgt cgtgccgtcg cgggtgcgca gcaggaccgg ggtcttctcg tcggccagct
     1501 cgacgaccag cgcgccctgg ctgatggtgg tcaggccctg ggcggcgagc cgctcgatga
     1561 gcggcggcag gtcgggctcg taggccgact caccgcgcac ctcgtcgtac tcgatcccga
     1621 ggatgtcgta gacccgccgg aactcggccc acgacacgtc gcggaaccgc tgccacagcg
     1681 cgcgggcctc gggatcgccg tcctccagcc gcttgaacca cgcgcgaccc tccgcctcga
     1741 gcgccggatc catcttcttc tcggcgttga accggacgta gagcgcgttg agcgcggtga
     1801 catcgagcgg ctcgaccggg ccccacttcg cccacgccgc gagcagcatg ccgtgcgtgg
     1861 tgccccagtc gcccaggaag ttgatgccga tgacgcggta gcccagcgcg cggtagatct
     1921 gggcgagcgc gtggccgatc gtcgtacccc ggatgtggtg gtaggcgagg tgcttggaga
     1981 tgttgggcga gccgtagtcg atgcagatcg tcttgtcggt gccgagctcg ccgggcacga
     2041 gccggccgtg ctgcgcgctg tcgatcagcc agcggtaggt cgtgtcgcgc cgggcgcgaa
     2101 aattgacgaa cggaccggcg gcggtcgcgc tctcgagcag gtcggtgggc tggaacgccg
     2161 ccgcgatcgc gcgggcgacc tcggcggggt tctgctggcg ggccttggcg atcgcgaagc
     2221 aaccgaccgc gaggtcgccg agctccgggc gggggggcgc ctcgaccttc agatcggtgg
     2281 ccgcaatgcc cgcgtgcgct gcgaccgcct cggctgcggc gcggtgcaga ggcgtgtgat
     2341 gcgtggccat gggcagcgca cgttagcacc gatcggcgcc actcgccaaa aggcctacgg
     2401 attctcggca gttgaattga cccctcggcg agcgccgtgc taccaacgaa cctgcatgat
     2461 gccctgcagg tcggcgcggc ggtgagcagt cccagacttc ttggggagga tccctcgtga
     2521 gcaagattgt cttgtgcgtc gatgacagcg cgacgatgca acaggtcgcg gacatcacct
     2581 tccgcggcac cgagttcacg tacgtcggcg cgcgcggctt cgacgagggg ctggacaagg
     2641 cccgtgcgca gaagcccatc gtcgtcctcg cggactcgca catgcccggc aagtcgggct
     2701 acgatctctg cctcgccctc aagaccgatc ctgcgacctc gaacatcccg gtggcgatcc
     2761 tggtcggcaa cgcggcgccc tacgacgccg cgcgcggcgt ccaggtcggc gccgacgcca
     2821 acctgcccaa gccgtgggac acgcagacga tgctcgagaa ggtcgccgag gtcgtcacca
     2881 aggccgccag cggggtcgcc aagccgggcc aggccgccgc ggccgcgccg gcccccg
//
LOCUS       DASVOR010001643         6472 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_484569,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001643 DASVOR010000000
VERSION     DASVOR010001643.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6472
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484569"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VF516_30775"
     CDS             complement(<1..345)
                     /locus_tag="VF516_30775"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8112172.1"
                     /translation="MDDVHTLIIGAGAVGLACAAVLARRGEPVAVLERHRRPGQETSA
                     RNSGVIHAGLYYPTGSLKARLCVAGRDLLYARCARAGVPHRRCGKIVVATDDAELARI
                     EAIARQAAVNGAE"
     gene            397..1704
                     /locus_tag="VF516_30780"
     CDS             397..1704
                     /locus_tag="VF516_30780"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF004856.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEX8112173.1"
                     /translation="MELTRGKQLAARLRQLVPGGCHTYNKGDDQFPELAPALIARGQG
                     CHVWDVDGNQFIEYGMGSRAVTLGHAYPKVVDAVRASLELGTNFTRPAPQELACAELF
                     LSLIDGADMVKFTKDSSTANTAALRLARRATGRDMVAVCADHPFFSYDDWFFCTTTSD
                     GGIPRVFSDQIVQFHYNDLASLRAQFAAHPGRIAAVILEPTRIEEPAPGFLAELVALS
                     HAEGAVVIFDEVITGFRWHARGAQHLYGVVPDLSVFGKAMANGFSMSALAGKREIMHW
                     GGRDRPDENVFLLSTTHGAETPSVVAAIATMEIYRSEPVVEHLHRVGARLAEGLRQAS
                     ARHGLTEHVMPVGRACHLFFGTRDPDGKPSQPFRTLFLQELIRRGVLGPSFVVSYSHQ
                     DEDIDRTIDAVDGALAVYARAMAARSVEGLLVGPPSRPVYGRR"
     gene            2010..3071
                     /locus_tag="VF516_30785"
     CDS             2010..3071
                     /locus_tag="VF516_30785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112174.1"
                     /translation="MPASRTDRKPATCHAAGALAVVLAVVLALGVSACASEDGSGRYP
                     FGPFDLAPSQEIVGTCVQISLHNPDALYIQSVELTTGPGFHHSNWLFVPDTAFPGNDG
                     TFSCDERGYNEGIAAALGGVLFAQSTQSPHETQAFPAGVAVRIPPQSKLIAQIHLLDA
                     QDVALHITPTIQLTMVAASQVKTLLSAISFADQALALPSHKQSRFSVECDLAPLYHQW
                     FQRDPDFKMYYGLAHYHALGTGLQLEAVKPDGTTAMVFTTAHRVGDTLGGPITPSFDM
                     TGYTRLRMTCDYSNPRDTVVGWGVGDQEMCVFLAFSDSKANWGGGTAATDPGPPTTGT
                     DGVVSYSQSCTVFALPADN"
     gene            complement(3151..4464)
                     /locus_tag="VF516_30790"
     CDS             complement(3151..4464)
                     /locus_tag="VF516_30790"
                     /inference="COORDINATES: protein motif:HMM:NF027579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEX8112175.1"
                     /translation="MPDADAGARMYALVEELYPICRSITGDGVRDTLARIERALPAPM
                     IRTEVPSGTPVLDWTVPDEWNIRDAWIADARGARVVDFRACNLHVVNYSVPVRARMTL
                     AELRPHLHALPDHPDWIPYRTSYYAPAWGFCLTQRQLDALPDGDYEVCIDSTLAPGHL
                     SYGELVLPGDVANDAPDGAANEVLLSTHVCHPSLCNDNLSGIAVATALARELAARPRR
                     RWTYRFVFAPGTIGAITWLAQHRDVVPRIRAGLTMMCLGSAHPFTYKRTLAGDAEVDR
                     AAAIVLRDRGAGDPLIDFFPYGYDERQYNSPGFRAPVGSVMRGRHGMFPEYHTSADNL
                     GFVSAERLAESLAVVRGIVDVLEANRRYANLAPYGEPQLGKRGLYRATGGTHIPDLNL
                     AMLWVLQLSDGSCDLVAIAERAKLPFATIRVAADHLRDAGLLANA"
     gene            complement(4490..5035)
                     /gene="rfbC"
                     /locus_tag="VF516_30795"
     CDS             complement(4490..5035)
                     /gene="rfbC"
                     /locus_tag="VF516_30795"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEX8112176.1"
                     /translation="MRFIEQRLPGVFLIELERRGDERGWFARSFCVDEMARAGLHTDF
                     PQGNASFNAQRHTLRGMHYNAAPHGEVKIVGCTAGAILDVAVDLRAGSPTRFQWCAVE
                     LTPASGAQLYIPAGFAHGFLTLADDTEVTYRMGARYVPEAARGVRWDDPRLAIAWPAR
                     PAVISERDASYRDLDPERIDA"
     gene            5108..6343
                     /locus_tag="VF516_30800"
     CDS             5108..6343
                     /locus_tag="VF516_30800"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8112177.1"
                     /translation="MSAPTCRFCDTRLAHTLCDLGMSPLCERILAAGQTNQMEPFYPL
                     KVWVCHKCLLVQLEQYVSAEDIFTEYAYFSAFSTAWLEHARNYVAMITQRLGLGPTSQ
                     VIELASNDGYLLQYFVERGIPALGIEPAVNVAEAARKRGVETLVRFFGRDLGVELAAA
                     GKRADLVIGNNVLAQVPDINSFVAGIAAVLTPTGTATLEFPHLQRLIEGNQFDTIYHE
                     HFGYFSLLAVEAIFAAHGLTIFDVEELWTHGGSLRIYARPTADTRRPVGPRVSELRAR
                     EEGLGFRDMATYAAFEERVRETKRRILELLIAAKRAGKRIAGYGAPGKGNTLLNYCGI
                     RTDFLDFTVDRNPYKHGRFLPGTHIPCFAPDKIDAEKPDYIFILPWNLKDEIMKQLAH
                     ARAWGAQFIIPIPEPQIVP"
BASE COUNT          929 a         2407 c         2191 g          945 t
ORIGIN      
        1 ctcggcgccg ttgaccgcgg cctggcgcgc gatcgcctcg atccgggcga gctcggcgtc
       61 gtcggtggcg accacgatct tgccgcaccg gcgatgcggc acgccggcgc gcgcgcaccg
      121 cgcgtacagc aggtcgcgac cggcgacgca cagccgggcc ttgaggctgc cggtcgggta
      181 gtacagcccg gcgtgaatca cgccgctgtt gcgcgccgag gtctcctgcc ccggccggcg
      241 gtggcgctcc agcacggcga ccggctcgcc gcggcgagcg agcacggcag cgcatgccag
      301 cccgaccgcg ccggcgccga tgatgagcgt gtggacgtcg tccaagactc gacctgcgga
      361 gcttgcccgc gcatgatacc aggcgggagg aaatccgtgg agctcactcg tggaaagcag
      421 ctcgccgcgc ggctgcgcca gctggtaccc ggcggctgtc atacctacaa caagggcgac
      481 gatcagttcc ccgagctcgc gcccgcgctg atcgcgcgcg gccagggctg ccacgtgtgg
      541 gacgtcgacg gcaaccagtt catcgagtat ggcatgggct cgcgcgcggt cacgctcggc
      601 cacgcctacc cgaaggtcgt cgacgcggtg cgcgcgtcgc tggagctcgg caccaacttc
      661 acgcggcccg cgccccagga gctcgcgtgc gccgagctgt tcctgtcgct gatcgacggc
      721 gccgacatgg tgaagttcac caaggacagc tcgaccgcga acaccgcggc gctcaggctc
      781 gcgcgccgcg ccaccggccg cgacatggtc gcggtctgcg ccgatcaccc gttcttctcg
      841 tacgacgact ggttcttctg cacgaccacg tccgacggcg gcatcccgcg cgtgttctcc
      901 gaccagatcg tgcagttcca ctacaacgac ctcgcgtcgc tgcgcgcgca gttcgccgcg
      961 caccccggcc ggatcgccgc ggtgatcctc gagccgacgc ggatcgagga gccggcgccg
     1021 ggcttcctcg ccgagctggt cgcgctgagc cacgccgagg gcgccgtcgt gatcttcgac
     1081 gaggtcatca ccgggttccg ctggcacgcg cgcggcgcgc agcacctgta cggcgtggtc
     1141 cccgatctct cggtgttcgg caaggccatg gccaacgggt tctcgatgtc cgcgctcgcc
     1201 ggcaagcgcg agatcatgca ctggggcggc cgcgaccggc ccgacgagaa cgtgttcctg
     1261 ctgtcgacca cccacggcgc cgagaccccg tcggtggtcg cggcgatcgc gaccatggag
     1321 atctaccgca gcgaaccggt cgtcgagcac ctgcaccggg tcggtgcgcg gctcgccgag
     1381 ggcctgcgcc aggccagcgc gcgccacggc ctgaccgagc acgtcatgcc ggtcggccgg
     1441 gcctgccacc tgttcttcgg cacccgcgat cccgacggca agccgtcgca gccgttccgc
     1501 acgctgttcc tccaggagct gatccggcgc ggcgtgctcg gcccgtcgtt cgtggtcagc
     1561 tactcgcacc aggacgagga catcgaccgc acgatcgacg cggtcgacgg cgcgctcgcg
     1621 gtctacgccc gcgcgatggc cgcgcgcagc gtcgaagggc tgctggtcgg gccgccgtcg
     1681 cgcccggtgt acggccggcg ctgagcgtgc accgcacgca tcgctcgcgc ggtgcaccga
     1741 cgagctccgc gacgtgctcg cggctgctgt cgatggcaga gatcgaacca tgctggcctt
     1801 ccgcggcacc gggcggcggt ccggcgacac cccgccgagc agaagtgcag cggcggcgca
     1861 atccttgtag gcccggtcac cggggatgca ttctactgtc cggtatccgg acggatgttg
     1921 aacattgcca ggccctgcaa cccgtcatcc tcgcgaagtt ccgtggcggg cttcttggca
     1981 gtgtgcccgg ctcatgcgag cctcgataca tgccggcttc acgcacggac cgcaaacccg
     2041 ccacctgcca cgccgccggg gcccttgccg tcgtccttgc cgtcgtcctt gccctcggcg
     2101 tgtccgcctg cgcgagcgag gatgggtcgg gccgctaccc gttcgggccg ttcgaccttg
     2161 cgccgagcca ggagatcgtc ggcacgtgtg tgcagatctc gctgcacaac cccgacgcgc
     2221 tctacatcca gtcggtcgag ctgacgaccg gtccggggtt ccatcactcc aactggctgt
     2281 tcgttccgga cacggcgttt cccggcaacg atgggacgtt ctcgtgcgac gagcgcggct
     2341 acaatgaggg aatcgccgcg gcgctcgggg gcgtgctgtt cgcccagtcg acccagtcgc
     2401 cgcacgagac ccaggcgttc ccggccggcg tcgcggtccg gatcccgccg cagagcaagc
     2461 tcatcgcgca gatccacctg ctcgacgccc aggacgtcgc gctgcacatc acgccgacga
     2521 tccagctcac catggtcgcc gcgtcccagg tcaagacgct cctgtccgcg atctcgttcg
     2581 cggaccaggc gctcgcgctg ccgagccaca agcaatcgcg gttcagcgtg gagtgcgatc
     2641 tcgccccgct ctaccatcaa tggttccagc gcgaccccga cttcaagatg tactacgggc
     2701 tcgcgcacta tcacgcgctc ggcaccggcc tccaactcga agcggtcaag cccgacggaa
     2761 ccacggcgat ggtgttcacg accgcgcacc gggtgggtga cacgctcggc ggtccgatca
     2821 ccccgagctt cgacatgacg gggtacaccc ggctccggat gacatgcgat tattccaacc
     2881 cgcgcgacac cgtcgtgggg tggggcgtcg gcgatcagga gatgtgcgtg ttcctcgcgt
     2941 tctcggactc gaaggccaac tgggggggcg gcaccgccgc cacagacccc ggccccccga
     3001 cgacaggcac cgacggcgtg gtgagctact cgcagagctg caccgtgttc gcgctccccg
     3061 cggacaactg aggcctccgc gcccgcacag ggtccgcctc gatccgcctc gcgccacctg
     3121 gtctcgctcg acgtcgcggt gaaccgtgcg ctacgcgttg gcgagcagcc cggcgtcgcg
     3181 caggtggtcg gcggccacgc ggatcgtcgc gaacggcagc ttcgcgcgct cggcgatcgc
     3241 gaccagatcg cagctgccgt cggacagctg cagcacccac agcatcgcga ggttgagatc
     3301 ggggatgtgg gtgccgccgg tcgcgcggta cagcccgcgc ttgccgagct gcggctcgcc
     3361 gtacggcgcc aggttggcgt agcgccggtt ggcctcgagc acgtcgacga tgccgcgcac
     3421 cacggcgagc gactcggcga gccgctcggc cgacacgaag ccgaggttgt cggcggaggt
     3481 gtggtactcg gggaacatgc cgtggcggcc gcgcatcacc gagccgaccg gcgcccggaa
     3541 ccccggcgag ttgtactggc gctcgtcgta gccgtacggg aagaagtcga tcagagggtc
     3601 gcccgcgccg cgatcgcgca ggacgatcgc cgccgcgcga tcgacctcgg cgtcgccggc
     3661 gagcgtgcgc ttgtacgtga acgggtgcgc gctgcccagg cacatcatcg tcaggccggc
     3721 gcggatccgc ggcacgacgt cgcggtgctg cgcgagccac gtgatcgcgc cgatcgtgcc
     3781 gggcgcgaac acgaagcgat aggtccagcg ccggcgcggc cgcgctgcca gctcgcgggc
     3841 cagcgcggtg gcgaccgcga tccccgacag gttgtcgttg cacagcgacg ggtggcagac
     3901 gtgggtcgac agcaggacct cgtttgccgc tccgtcgggc gcatcgttcg ccacatcccc
     3961 tggcagcacc agctcgccgt agctcaggtg gccgggcgca agcgtcgagt cgatgcagac
     4021 ctcgtagtcg ccgtcgggca gcgcgtcgag ctggcgctgg gtcaggcaga atccccaggc
     4081 cggggcgtag tacgacgtgc ggtacgggat ccagtccggg tgatcgggca gcgcgtggag
     4141 gtgcggccgg agctcggcga gcgtcatccg cgcccgcacc ggcacgctgt agttgacgac
     4201 gtgcaggttg cacgcccgga agtcgaccac gcgggcgccg cgggcgtcgg cgatccacgc
     4261 gtcgcggatg ttccactcgt cgggcacggt ccagtcgagc accggcgtgc cgctcgggac
     4321 ctcggtgcga atcatcggcg ccggcagcgc gcgctcgatc cgcgccagcg tgtcgcgcac
     4381 cccgtcgccg gtgatgctgc ggcagatcgg gtacagctcc tcgaccagcg cgtacatccg
     4441 cgcgccggcg tccgcgtctg gcatctcagg catcgattca ggccccgtct caggcatcga
     4501 tgcgctccgg atcgaggtcg cgatagctcg cgtcgcgctc cgagatcacc gccgggcgcg
     4561 ccggccacgc gatcgccagc cgcggatcgt cccaccgcac gccgcgcgcc gcctcgggca
     4621 cgtagcgcgc ccccatccgg tacgtgacct cggtgtcgtc ggccagggtc agaaagccgt
     4681 gcgcgaaccc ggccgggatg tagagctgcg ctccggacgc cggcgtgagc tcgaccgcgc
     4741 accactggaa gcgcgtcggc gagcccgcgc gcaggtcgac cgcgacgtcg aggatcgccc
     4801 ccgcggtgca gccgacgatc ttgacctcgc cgtggggcgc ggcgttgtag tgcatgccgc
     4861 gcagcgtatg gcgttgggcg ttgaacgacg cgttgccctg cgggaagtcg gtgtggagcc
     4921 cggcccgggc catctcgtcg acgcagaagc tgcgggcgaa ccagccgcgc tcgtcgcccc
     4981 gccgctcgag ctcgatcagg aacacgcctg gcaagcgctg ctcgatgaac ctcacggggc
     5041 gagcttaccc cgccgatcgc aaccgcgcgg cctccccggc gcccgggcgg cctgatagcg
     5101 tgctggcatg tccgcgccga cctgccggtt ctgcgacacc cgcctggccc acacgctgtg
     5161 cgacctcggg atgtcgccgc tgtgcgagcg catcctcgcc gccggccaga ccaaccagat
     5221 ggagccgttc tacccgctca aggtctgggt ctgtcacaag tgcctgctgg tccagctcga
     5281 gcagtacgtc tccgccgagg acatcttcac cgagtacgcc tacttctcgg cgttctcgac
     5341 cgcgtggctc gagcacgcca ggaactacgt cgcgatgatc acccagcggc tcggcctcgg
     5401 cccgaccagc caggtgatcg agctcgcgtc caacgacggc tacctgctgc agtacttcgt
     5461 cgagcgcggc atcccggcgc tcggcatcga gccggcggtc aacgtcgccg aggccgcccg
     5521 caagcgcggc gtcgagaccc tggtccggtt cttcggccgc gacctcggcg tcgagctcgc
     5581 cgcggccggc aagcgcgccg acctggtgat cggcaacaac gtcctggccc aggtccccga
     5641 catcaactcg ttcgtcgccg gcatcgccgc ggtcctcacg ccgaccggca ccgccacgct
     5701 ggagttcccg cacctccagc gcctgatcga gggcaaccag ttcgacacga tctaccacga
     5761 gcatttcggc tacttctcgc tgctcgcggt cgaggcgatc ttcgccgcgc acggcctcac
     5821 gatcttcgac gtcgaggagc tgtggaccca cggcggctcg ctgcggatct acgcgcgccc
     5881 caccgccgac acccgccgcc cggtcggccc gcgggtcagc gagctgcgcg cccgcgagga
     5941 ggggctcggg ttccgcgaca tggcgaccta cgcggcgttc gaggagcgcg tccgcgagac
     6001 caagcgccgc atcctcgagc tgctgatcgc cgccaagcgc gccggcaagc ggatcgccgg
     6061 ctacggcgcg cccggcaagg gcaacacgct gctcaactac tgcggcatcc gcaccgactt
     6121 cctcgacttc accgtcgatc gcaaccccta caagcacggc cggttcctgc ccggcacgca
     6181 catcccgtgc ttcgcgcccg acaagatcga cgccgagaag cccgactaca tcttcatcct
     6241 gccgtggaac ctcaaggacg agatcatgaa gcagctcgcc cacgcccgcg cgtggggcgc
     6301 ccagttcatc atcccgatcc ccgagcccca gatcgtgccg tgatcctgcg cctggcgcca
     6361 gatttctccc gccccggctc ccgccccggg gcccgccccg gtggcgcgcc gctccgcgtg
     6421 ctgctgctcg gcgcccacgc cgacgacatc gagatcggct gcggcggcgc cg
//
LOCUS       DASVOR010001644         2437 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_543095,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001644 DASVOR010000000
VERSION     DASVOR010001644.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543095"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2437)
                     /locus_tag="VF516_30805"
     CDS             complement(<1..>2437)
                     /locus_tag="VF516_30805"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEX8112178.1"
                     /translation="MDERANRAVIEAFVRLYEEGLIFRAKRMINWDPVSETVVSDLEV
                     DTADENGSLWEIRYPIVGGASAAGAIAGAPGDAITVATTRPETMLGDTAVAVHAGDER
                     YKHLHGQLVELPLTGRRIPIVPVDLMIDGKPWPDPTFGSGAVKVTPAHDPSDYEVSQH
                     VGLPVLQVIDAKGRICDPAPAKYVGMPVADARKAVVADLEAAGLLGAVKDYRVPRGKS
                     QRSGAVIEPMLMEQWWVKAAPLAEKAIAAVEQGKTRFVPEHETKTFMHWMTHIKDWCI
                     SRQLWWGHRIPAWHCAQCAGITVAREAPAACSHCGSAAIRQDEDILDTWFSSGLWPFS
                     TLGWPDRTRELATFYPNNVLVTGPDILFFWVARMMMMGLHFLGKVPFRTVYLTSIITD
                     ENGAKMSKTKGNGIDPLDVVFGASLDQLLERVDTEKPPVDLEQLKRTVRKNFPKGIPA
                     MGADALRFAMAALNTTLRIRLSVDRVEKYRNFINKLWNASRFALMNLDGYDPERFEAQ
                     LSTPAGRAALGMPERWILSRLQAVVGDVDAALESFRFNDAAGAIFHFVWDELCDWYIE
                     IAKPHLHTGSEFENDAPRTARRHVVQGVLATALETTMRLMHPFSPFVTEEIWQKLPKP
                     PQLPGSLMITVFPRSDPSWTDSEAEGEMQLLQEVAVGCRMLKQTYGVSPAQSTDVELR
                     VGNAEQRAVIERHLGLIERAAKVTARVSADIAAPAPSGSAKEVVGGDLEIVMLLGGLI
                     DIAAEKSRIAKDIAKADKEIAVLDKKLGNAEFLARAPEDVVAEQRARLAEEQARRRRL
                     ADALATLSSGPGDA"
BASE COUNT          338 a          859 c          853 g          387 t
ORIGIN      
        1 gcatcgcccg ggccgctcga cagcgtggcc agcgcgtcgg ccagccgccg ccggcgggcc
       61 tgctcctcgg cgagccgggc gcgctgctcg gcgaccacgt cctcgggcgc gcgcgccagg
      121 aactcggcgt tgccgagctt cttgtccagc accgcgatct ccttgtcggc cttggcgatg
      181 tccttggcga tccggctctt ctcggccgcg atgtcgatca gcccgccgag cagcatgacg
      241 atctcgagat cgccgccgac gacctccttg gccgagccgg acggcgccgg cgccgcgatg
      301 tcggcggaca cccgggcggt gaccttggcc gcgcgctcga tcagcccgag gtggcgctcg
      361 atcaccgcgc gctgctcggc gttgccgacc cgcagctcga cgtcggtcga ctgcgcgggc
      421 gacacgccgt aggtctgctt gagcatgcgg cagccgaccg cgacctcctg gagcagctgc
      481 atctcgccct cggcctcgct gtcggtccac gacggatcgc tgcgcgggaa cacggtgatc
      541 atcagcgagc cgggcagctg cggcggcttg ggcagcttct gccagatctc ctcggtgacg
      601 aacggcgaga acgggtgcat gagccgcatc gtggtctcga gcgcggtggc gagcacgccc
      661 tggacgacgt ggcggcgcgc ggtgcgcggt gcgtcgttct cgaactccga cccggtgtgg
      721 aggtgcggct tggcgatctc gatgtaccag tcgcacagct cgtcccacac gaagtggaag
      781 atcgcgccgg cggcgtcgtt gaaccggaac gactcgagcg cggcgtcgac gtcgccgacc
      841 accgcctgca gccgcgacag gatccagcgc tccggcatgc cgagcgcggc gcgccccgcc
      901 ggggtcgaca gctgggcctc gaaccgctcg gggtcgtagc cgtcgaggtt catcagcgcg
      961 aaccgcgacg cgttccacag cttgttgatg aagttgcggt acttctcgac ccgatcgacc
     1021 gacagccgga tccgcagcgt ggtgttgagc gcggccatcg cgaagcgcag cgcgtcggcc
     1081 cccatcgccg ggatgccctt ggggaagttc ttgcggaccg tgcgcttgag ctgttccaga
     1141 tcgaccggcg gcttctcggt gtcgacccgc tcgagcagct ggtcgagcga cgcgccgaac
     1201 acgacgtcga gcggatcgat gccgttgccc ttggtcttgg acatcttggc gccgttctcg
     1261 tcggtgatga tcgaggtcag gtacacggtg cgaaacggca ccttgcccag gaagtgcagg
     1321 cccatcatca tcatccgcgc gacccagaag aacaggatgt cggggccggt gaccaggacg
     1381 ttgttgggat agaacgtcgc gagctcgcgg gtccggtccg gccagcccag ggtcgagaac
     1441 ggccacagcc ccgagctgaa ccaggtgtcg aggatgtcct cgtcctggcg gatcgccgcg
     1501 ctgccgcagt ggctgcacgc cgcgggcgcc tcgcgcgcga cggtgatccc ggcgcactgc
     1561 gcgcagtgcc acgccgggat ccggtggccc caccacagct ggcgcgagat gcaccagtcc
     1621 ttgatgtggg tcatccagtg catgaacgtc ttggtctcgt gctcgggcac gaaccgggtc
     1681 ttgccctgct cgaccgcggc gatcgccttc tcggcgagcg gcgcggcctt gacccaccac
     1741 tgctccatca gcatcggctc gatcaccgcg cccgagcgct ggctcttgcc gcgcggcacc
     1801 cggtagtcct tcaccgcgcc gagcaggccg gcggcctcga ggtcggcgac caccgccttg
     1861 cgggcgtcgg cgaccggcat cccgacgtac ttcgccggcg ccgggtcgca gatccggccc
     1921 ttggcgtcga tcacctggag gaccggcagg ccgacgtgct gcgagacctc gtagtcgctc
     1981 gggtcgtgag ccggggtcac cttgaccgcg cccgagccga acgtggggtc gggccacggc
     2041 ttgccgtcga tcatcagatc gaccgggacg atcgggatcc gccggccggt cagcggcagc
     2101 tcgacgagct ggccgtgcag atgcttgtag cgctcgtcgc cggcgtggac cgcgaccgcg
     2161 gtgtcgccga gcatggtctc gggccgggtg gtcgcgacgg tgatcgcatc gcccggcgcg
     2221 ccggcgatcg ccccggcggc gcttgcgccg ccgacaatgg gataccgaat ctcccacagc
     2281 gagccgttct catcggcggt gtcgacctcg aggtcggaca ccacggtctc gctgaccgga
     2341 tcccagttga tcatccgctt ggcgcggaag atcaggccct cctcgtacag ccgcacgaac
     2401 gcctcgatga cggcgcggtt ggcgcgctcg tccatcg
//
LOCUS       DASVOR010001645         1739 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_337974,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001645 DASVOR010000000
VERSION     DASVOR010001645.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337974"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="VF516_30810"
     CDS             <1..322
                     /locus_tag="VF516_30810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112179.1"
                     /translation="PIEELSLPLGGASSLQSAHDAGQPFVGTPPSAARPVEKQLWAAL
                     GTQPEPFEVLVVPVLVKQRAVNLVYTHIASSPPPNQLVTELGALATRAQASYLRLIRQ
                     ARGS"
     gene            complement(872..1321)
                     /locus_tag="VF516_30815"
     CDS             complement(872..1321)
                     /locus_tag="VF516_30815"
                     /inference="COORDINATES: protein motif:HMM:NF017868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HEX8112180.1"
                     /translation="MAERRRPTDSLEARRTTGSNEVLDRLFVYSTLRTGQTARSLITN
                     HVARCVPASTPGAIYAFPMGYAGLVEAESPCRVIGEVLWLTDLPATFGLLDAYEGDDF
                     ARVIKQIELDTGEALWAWIYVLTDPDTARHGTRIEHGDWERYVREQR"
     gene            complement(1331..>1739)
                     /locus_tag="VF516_30820"
     CDS             complement(1331..>1739)
                     /locus_tag="VF516_30820"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: protein motif:HMM:NF014347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HEX8112181.1"
                     /translation="LADPLAEAAGARGAEMTRLGARPGARTSALELLAAFGGPETGVL
                     AGLMLAAASTNVPVILDGYATGAAALIAAALAPSAAGYLVAAHTGSFTHARILAHLGL
                     HPMFEVGLGHGEGTGAAMALALIDHMAAVASQG"
BASE COUNT          272 a          644 c          569 g          254 t
ORIGIN      
        1 gccgatcgag gagctgtcgc tgccgctcgg gggcgcgtcg tcgctgcagt cggcgcacga
       61 cgccggccag ccattcgtcg ggacgccgcc gtccgcggcg cgcccggtcg agaagcagct
      121 gtgggcggcg ctcggcaccc agcccgagcc gttcgaggtg ctcgtcgtgc ccgtgctggt
      181 caagcagcgc gcggtcaacc tggtgtacac ccacatcgcc agcagcccac cgccgaacca
      241 gctggtcacc gagctcggcg cgctcgccac gcgggcccag gcgtcttact tgcgcctgat
      301 tcgccaggcg cgcggctcgt gatcgcccgg tcgctgtatc actcccgcac actggagcct
      361 cggccccctc cggcaatctc gctcgcgggt caagcgttcc cgacgaaaaa ttaggcggcg
      421 cgatcattca taacacctcg atagtacgaa ggaagttacg tcgatcggaa gggctggccg
      481 ggaacttgac tccgcccgcc aaagagggaa agtgcagccc gtgccacaac aaatgtccat
      541 gccgaaccag ggctgcaaag tcgggctcgg cacgatcgcc gctgcgttag ctgccctctc
      601 gatggccgtg ctcggacact ttttcatctg aactctgatc cgtcgatccc catccgacgc
      661 acccggccgc cggttcacct gggacctgcc cccgcgaccg accttgccgg tcaggacgcg
      721 cgccggatgc cccgccggta gacaccgcat cgccttttgc ctgtcatcgg cacgccgtca
      781 gagcggcacc gtcgggcagg cgctgacagg cgaaaaccgg agatccgcac cggacgtccg
      841 cgatcgcgga accgatccga gcaatgacgt cctaacgctg ctcgcgcacg tagcgctccc
      901 agtcgccgtg ctcgatccgc gtgccgtgcc gtgccgtatc gggatcggtc agcacataga
      961 tccacgccca cagcgcctcg ccggtgtcga gctcgatctg cttgatcacg cgggcgaagt
     1021 cgtcgccctc gtaggcgtcg agcaggccga acgtcgccgg caggtcggtc agccacagca
     1081 cctcgccgat cacgcggcac ggcgactcgg cctcgaccag gccggcgtag cccatcggaa
     1141 aagcgtagat cgcgccgggc gtggaggccg ggacgcagcg cgcgacgtgg ttggtgatca
     1201 gcgagcgcgc cgtctggccg gtccgcagcg tggagtagac gaacagccga tcgagcacct
     1261 cgttcgagcc ggtcgtgcgc cgggcctcca ggctatcggt cggtcgcctc cgctcggcca
     1321 tcccgacgat ttaaccctgg ctcgccaccg ctgccatgtg atcgatcaac gccagcgcca
     1381 tcgccgcgcc ggtcccctcg ccgtggccga ggccaacctc gaacatcgga tgtagaccga
     1441 ggtgggcgag gatccgggcg tgggtgaacg acccggtgtg cgccgccacc aggtagccgg
     1501 ccgcgctcgg cgcgagggcc gccgcgatca gcgccgctgc accggtggca taaccgtcga
     1561 gaataaccgg gacattggtc gacgccgcgg cgagcatcag gccggcgagc acgccggtct
     1621 cggggccgcc gaatgccgcg agcagctcca gcgcgctcgt tcttgccccc ggcctcgccc
     1681 ccagcctcgt catctcggcg ccccgggcgc cggccgcctc ggccagcggg tccgccagc
//
LOCUS       DASVOR010001646         6867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_455049,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001646 DASVOR010000000
VERSION     DASVOR010001646.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455049"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            804..3890
                     /locus_tag="VF516_30825"
     CDS             804..3890
                     /locus_tag="VF516_30825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112182.1"
                     /translation="MSFEPVDERRCGRRWYLAMVFSSMATIAACSEDAPHPAHAGQLA
                     GQVVLSGPLRGAKVSVDQLDYSVKSSVTVRAHVADTMTDDDGRFSVDTGTYSGLLLVT
                     ARGGSFTDLATGATLQLDPTAGLESIEPLDLLEQRDDALVSPVGHLIAARTRSRMAQL
                     HDLVMAERDAEDHLGRHFGNVPWMRVKLASLGSPATSPTESVRAALVQDALSFLVQDI
                     AQAAGASPQEVNVLTLTQQLAADIRQGPFDGNDGNSPAPGDGLQVGVCGPIAGCSAPP
                     AGTCALGACRPLCDLYAGTPRALLAGEVTKVIQSSMLNHTGLTTGDVLAVARSMADNV
                     DEDLFGSACVEALDRIPPRLDWAAPTPADGAYVRGSFAVRAIATDDTDPRPAVIIEGY
                     PNVPADSAASATIDSTQLQLPDGMLVVIARARDMAGNTSTLQRAVRVDNTPPALTLDP
                     SRYFVDGSTWWTADASPTLSGTVSDAAPVTVITSVPSGTTGAAVTGAIWSGGVAGTLD
                     LSGADVMVTATDAAGNRTQITQHVRSDITPPQLTFGTSTVRDEAAETPTFDGNEAPVH
                     VHTGSTVHLENATGCPAVTKFSYLLGASAPPYGSEVTGQGGPDPNPVQYVLLAEDDGV
                     GIASGSVQYRVGLQLAGTGATTWVLDWTSAGTPNRIGPGVDQHKIGIFSDQVAQLATT
                     EGVYHVEFRATDRLSRTTTVARCFELHLRAPPLHFQTPGQLAPDPDPIPVDHAYRLLS
                     LSLTQKAPFRAIAARLLNSNSSGASVLDEDITNGTANAVYLDVTVVKPATINVWQTFK
                     LSNAFLPQDVTINCTHATGPPPDPRCNRPGPPGPTYDTTPVKQSEVPISGLTFPVKIF
                     EVDGNLAPATEVPCVVCGVNDHWGFVLPSRPGTAEAAAPPRRFRAMTMVSQVSALWPS
                     DSNSPATEPFADTSVKAMVDNMIKVVTITGRRDPSSTGCTAHKTHLLPGGIESDTCLR
                     MTTITPYRALTKMHLKTSDETKSSYATAPTPMIVPVGVTSRQVMGFTWESASDPLP"
     gene            3913..4305
                     /locus_tag="VF516_30830"
     CDS             3913..4305
                     /locus_tag="VF516_30830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112183.1"
                     /translation="MSISRSVTLLLAGAAFGAAAVLSCSDNSPQHTDAATCNCEPPIA
                     GRLVTFESTVQTIQPGELSGAGTACDTGMQFISGGCTAEDPTVLQDIVVQQFGFDKTF
                     FSWGCAFKNNARVPVRVKATVLCLKPGA"
     gene            4412..5467
                     /locus_tag="VF516_30835"
     CDS             4412..5467
                     /locus_tag="VF516_30835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112184.1"
                     /translation="MIGLTVFAIGCGDNEEDPQYEVISPSICSTADGSVDGIFLLREH
                     EFAIVCSGSTYSVYRFDERTHTSELIYRGPEAFDSGAAGDFLYSIGYDSLVHKPIIQG
                     LRIAAGQASPLPILTATSNALESVDSIGLSSSYIYWSWLGVDSAITPQRGLSRVPLTG
                     GAEEIIEPMSFIADYQVISELMVIGDGDMYSYGYPKLEMGDYSVAIAHFSVGDRRSET
                     IFQQAQGAPAPRPWIGATSTHAIFSDTSVVPPKVYSLEGSKVYPEGKLRCPLDDRSVI
                     TGNVIVSYNFVACAPHDVTLVATDISSGEERYYRAQPGDLSVPDVPDFDKGIIGARNG
                     WIYWANQNQLARVRAVH"
     gene            5576..6415
                     /locus_tag="VF516_30840"
     CDS             5576..6415
                     /locus_tag="VF516_30840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112185.1"
                     /translation="MAPRSEAEFLVPPQGGAFEVPIHAREVCILSFAERLSSRALASS
                     PDFEIKSWGEDGVAVRAVGETAKTTTLAVATTSGAVKVNVTLTVVPSDRPAYTLVRFK
                     AVSAEEAFDAQVRAEVGKRVAPLEAELAGLRQGLEIRAREQAEALIAERLLRRNEAIA
                     LAAHERNDDHVIVHVTRGVLIGDDGYLMFEIENRSGSAYRLAAVRVQADGRDVHGPVR
                     LTSPGADRDPTVIGVVPARTSGHGIVVVHAVDQVLGKNLAMTIAGPAGSPPIRLDRGI
                     VFR"
     gene            6412..>6867
                     /locus_tag="VF516_30845"
     CDS             6412..>6867
                     /locus_tag="VF516_30845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112186.1"
                     /translation="MTAARAVRSRRWPRYGRPLLLLVGLLAPTGLQARGAVADRYEAT
                     VSIRPIGSVGRFTEDVAGDARGATASTYGGGGELGLSYGVRNWLDLGAELAAAGFTRA
                     TYRDAIVTIMGIPATGRVTRTARLSQLRAGATFRLGLAWVPTVYVGAGLS"
BASE COUNT         1190 a         2242 c         2255 g         1180 t
ORIGIN      
        1 ggtgcgcagc cagcggaggt acagcgcctc ggcgtcccgc ggatcgagcg cgcgctgcac
       61 ggcgcgcacg tacagggcgt gggccgtatg cgacggcacc tcgagctcgc gcgcggcccg
      121 ctgccactcg aactgcgcgt cccggtcgtc gccgcggaac cgccgctcca cgctcaggcg
      181 cccccctcac cgcggccgcg ggggcgcgga ccggccggcc ggatcgatct ccccatggca
      241 gatctcgacg cagccggccc gcgatctttc ggtccgattc acccgcgccg gcccggcacc
      301 gccggggcgc gagcagaaac gccgggttgg cgcgcgccga gtcgagccac tgcgcacttg
      361 aatcccgtcc gtgacgggcc cgagcaagca tccgtgaagc tggctgtcga tccgtgcctc
      421 gagctgccgc ggggcgatgt cgtgcatggg aagtgcgcca cgtgggagcg gacgcgcatg
      481 gcaggctgcc gtacggaccg aggccgggac gagcgccgct gacacggtag agagctgcca
      541 ttcacgcgag gcaacgctgc ctcgcgccct gccttcgccc gggtgctcgc gatgcgtgac
      601 cgatcgggcc gtcgccgtcg ccacggcgca catcgggtac gcgcgagcgt gagcaccccc
      661 actcgatgca caacagacta cctaccgggg tagagatgtc gggaactgaa aacagcaaac
      721 cgcctcactg gcgtcggtcc agcggcgtgt gggcatggtc gggcgtgaat gctatccgag
      781 gattccggag gactcggccc gcaatgagct tcgaaccggt ggatgagcgg cgatgcggtc
      841 gccggtggta tctcgcgatg gtcttcagct cgatggcgac gatcgccgcg tgcagcgagg
      901 acgcacctca tccagcgcac gctggccagc tcgccggcca ggtcgtcctg tcgggcccgc
      961 ttcgcggcgc caaggtctcc gtcgatcagc tcgactacag cgtgaagtcg tccgtgacgg
     1021 tccgcgcgca cgtggccgac acgatgaccg acgacgatgg acggttcagc gtcgacaccg
     1081 ggacctacag cggcctgctc ctcgtgacgg ccaggggcgg ctcgttcacc gacctggcga
     1141 ccggcgcgac cctccagctc gacccgaccg cgggcctcga gtcgatcgag ccgctcgacc
     1201 tcctcgagca gcgagacgat gccctggtga gcccggtcgg gcacctgatc gccgcgcgga
     1261 cgcgctccag gatggcccag ctccacgacc tcgtgatggc ggagcgcgac gccgaggatc
     1321 acctggggcg gcacttcggc aacgtaccct ggatgcgcgt caagctcgct tcgctcggtt
     1381 cgccggcgac gagcccgacc gaatccgttc gagcggcgct cgtccaggac gcgctgagct
     1441 tcctggtgca ggacatcgcc caggcagcgg gtgcatcgcc ccaggaggtc aacgtcctca
     1501 cgctgaccca gcagctcgcg gccgacatcc gccaggggcc gttcgatggc aacgacggca
     1561 actcgccagc ccctggcgac ggtttgcagg tcggcgtctg cggaccgatc gcaggctgca
     1621 gcgccccgcc ggccggcacc tgcgcactcg gcgcgtgccg cccgctgtgc gatctgtacg
     1681 cgggcacccc gcgcgcgctc ctcgcgggcg aggtcaccaa ggtcatccag tcgtccatgc
     1741 tcaatcacac cggtctcacc accggcgatg tgctggccgt ggcgcgctcg atggcagaca
     1801 acgtcgatga ggacctgttc ggctcggcgt gcgtcgaagc gttggaccgg atcccgccgc
     1861 gactcgactg ggccgcgccc acgccggcgg acggcgcata tgttcgcgga tcgttcgctg
     1921 tccgcgcaat cgcgaccgac gacaccgacc ctcggcccgc ggtgatcatc gagggctatc
     1981 ccaacgtgcc tgcggacagc gcggcgtccg cgacgatcga ctccacacag ctgcaactcc
     2041 ccgacgggat gctcgtcgtc atcgcacgag cgcgcgacat ggcaggcaac acctcgaccc
     2101 tccagcgcgc cgtccgcgtc gacaacacgc caccggcgct cacgctggat ccatcccgtt
     2161 acttcgtgga cgggagcacg tggtggaccg cggacgcgtc gccgaccctg agcggcacgg
     2221 tctccgacgc agcgcccgtg acggtcatca cgagcgtgcc cagcgggacg acgggtgcgg
     2281 ccgtgaccgg agcgatctgg tcaggcggtg tggccggaac gctggacctg tccggagccg
     2341 acgtgatggt caccgccacg gacgcggctg gcaaccgcac gcagatcacg cagcatgtcc
     2401 gctccgacat cacgccgcca cagctgacat ttggcacgag caccgtccgg gacgaagcgg
     2461 ccgagacccc gacgttcgat ggcaacgagg cacccgtgca tgtccacacc ggctcgacgg
     2521 tccacctgga gaacgccacg ggatgcccgg ccgtgaccaa attcagctac ctgctgggcg
     2581 catcggcgcc gccctacggg agcgaggtca ccggtcaggg cggtcccgat cccaacccgg
     2641 tgcagtacgt cctcctcgcg gaggacgacg gtgtcggcat cgcatctggc tccgtgcagt
     2701 accgggttgg gctccagctc gccggtaccg gtgccacgac gtgggtcctc gactggacat
     2761 ccgctggcac gccgaaccgc attggccccg gcgtcgacca gcacaagatc ggcatcttct
     2821 cggaccaggt cgcgcagctc gcgaccaccg agggggtcta ccacgtcgag ttccgggcca
     2881 ccgaccggct ttcccggacc acgaccgtgg cccgttgctt cgagctccac ctccgtgcgc
     2941 ccccgctcca ttttcagacc ccggggcaac tcgcgcccga tccggatccg attcccgtcg
     3001 atcatgccta tcggctcctg tcactcagcc tgacgcagaa ggcgccattc cgggcgatcg
     3061 ccgcacgtct gctcaacagc aattccagcg gagcgtcggt cctcgacgag gacatcacga
     3121 atggaacggc aaatgccgtg tacctcgacg tgaccgtggt taaaccagcc acgatcaacg
     3181 tatggcaaac cttcaagctc tcgaatgcct tcctgcctca ggacgtcacc atcaactgca
     3241 cgcacgccac aggccctcct cctgatcccc gctgcaatag acctgggcca cctgggccga
     3301 cctatgacac aacgcccgtt aaacagagcg aagtgccgat cagcgggctg accttcccag
     3361 taaagatctt tgaagtggat ggcaatcttg ctcccgccac agaggtgcct tgtgtagtct
     3421 gtggagtcaa cgaccattgg ggcttcgtgc tcccgtctcg tcctgggacc gcggaggctg
     3481 ctgcgccacc gcgcagattc agggcaatga ccatggtcag ccaggtctcg gccctgtggc
     3541 cgagtgacag taattcccct gcgacggagc cgtttgctga tacctcggtc aaagctatgg
     3601 tcgataatat gatcaaggtc gtgaccatca ccggaaggag agatccatcc agcaccgggt
     3661 gcacagccca caagacccat ctattgcccg gtgggatcga gagtgacact tgtttgagaa
     3721 tgacaacaat cacaccgtat cgagcactga cgaagatgca tctaaagact tccgatgaga
     3781 cgaaatcaag ttatgcgacg gcgccgacgc cgatgattgt ccccgtaggc gttacatcga
     3841 gacaggtaat gggattcacg tgggagagcg cttcggaccc gctgccatag actcttaccg
     3901 aggaggagaa tcatgagtat ctcgagatct gttaccctgc tccttgctgg ggctgccttc
     3961 ggcgcagcag ccgtgctgtc ctgctctgac aattcgccac aacacaccga cgcggcaacg
     4021 tgcaactgcg agcccccgat cgcggggcgc ctggtcacct ttgaaagtac cgtacaaacg
     4081 atccaacccg gcgagttgag cggggctggc accgcgtgcg atactggaat gcaatttata
     4141 tcgggcggct gcaccgctga agatccgacc gttttgcagg atattgtcgt tcaacagttt
     4201 ggattcgata agaccttctt tagctggggt tgcgcattca agaataatgc cagggttcct
     4261 gtgcgcgtga aggccaccgt cttgtgcctg aagcctggcg cgtagctcct gaactccttg
     4321 ctctcaattt ttgagggtag tcgtgatatt gatccacctc atagtcacat gcttgggagt
     4381 ctccatgaca cgaaaaccac gttcgcttgg ggtgatcggc ctgaccgtct ttgcgatagg
     4441 gtgcggtgat aacgaggaag atccacagta cgaggtcatc agtccgagta tttgctcgac
     4501 agcagatgga agcgtggatg gcatattcct cctgcgcgaa cacgagtttg cgatcgtttg
     4561 ctctggctca acatattcgg tgtatcgttt tgacgagcgt acacacacct cggagttgat
     4621 ctacaggggc cctgaagcct tcgacagtgg cgcggcagga gacttcctgt attcaattgg
     4681 ttatgattct ttggtgcaca agccgatcat ccaaggcttg cgcatcgctg ctgggcaagc
     4741 aagcccgttg ccaattctga cagcgacctc caatgcgctc gaatcggtag acagcattgg
     4801 cctctcaagt tcttacatat attggtcatg gctcggagtg gattcagcga ttactcccca
     4861 acgtggattg tcgcgtgttc cgttgaccgg tggagccgag gagattatcg aaccgatgtc
     4921 atttatagcc gattatcaag ttataagtga gctcatggtc atcggtgatg gcgatatgta
     4981 ttcgtatggt tacccgaaat tggaaatggg cgactacagt gtagcgattg cacacttctc
     5041 cgtgggggat cgacgatctg agacgatttt tcagcaagcg cagggcgctc ctgcgccgag
     5101 accctggatc ggagctacct cgacgcacgc aatattctct gacacatcag ttgtgccacc
     5161 gaaggtctac tccttggagg gcagcaaggt gtatcccgag ggcaagttgc gatgcccact
     5221 cgacgaccgg tcggtgatca cggggaacgt catcgtttcg tacaactttg tcgcgtgcgc
     5281 ccctcatgac gtgacactcg tcgcgacgga catcagcagc ggcgaggaga gatactatcg
     5341 ggcgcaacct ggtgatctgt cggtgccgga tgtccccgat tttgacaagg gtattatcgg
     5401 ggcccgcaat gggtggatct attgggctaa tcaaaatcag ctggcccgtg tgcgggcggt
     5461 gcactagatg cggcgctgct ctacaagagc gtatggctgt gttgttgcct ggacgatcgt
     5521 ggtctgtgga gtggccgatg gttctcatgc ggtcgcgcaa ccgggggctg cagaaatggc
     5581 gcctcggtcc gaggcggagt tcctcgtgcc gccgcagggc ggggcgttcg aggtgccgat
     5641 ccacgctcgt gaggtgtgca tcctgtcgtt cgccgagcgg ttgagcagca gggcgctggc
     5701 gagctcgccg gacttcgaga tcaagtcgtg gggcgaagac ggcgtggcgg ttcgcgcggt
     5761 cggcgagact gccaagacga ccacgctcgc cgtggcgacg acctcggggg cggtgaaggt
     5821 caacgtgacc ctgacggtcg tgccctcgga caggccggcg tacacgctcg tgcggttcaa
     5881 ggcggtatcg gccgaggagg cgttcgatgc gcaggtccgg gccgaggtcg gcaagcgcgt
     5941 cgcgccgctg gaggccgagc tcgccgggct gcgccagggg ctcgagatcc gggcgcgcga
     6001 gcaagccgag gcgctgatcg ccgagcggtt gctcaggcgg aacgaagcga tcgcgctggc
     6061 cgcgcacgag cgcaacgacg accacgtgat cgtgcatgtc acgcggggcg tgctgatcgg
     6121 cgatgacggg tacctgatgt tcgagatcga gaaccgctcg ggcagcgcgt accggctcgc
     6181 cgccgtgcgc gtccaggcgg atggccggga cgttcacggg ccggtgcgcc tgacgtctcc
     6241 gggcgcggat cgcgatccga ccgtgatcgg cgtcgtgccg gcgcggacca gcggccacgg
     6301 catcgtcgtc gtccacgcgg tcgaccaggt gctcggcaag aacctcgcga tgacgatcgc
     6361 cggtcccgcc ggctcgccgc cgatccgcct ggatcggggg atcgtctttc gatgaccgca
     6421 gcgcgcgccg ttcggagccg ccggtggccg cgatacggga ggccgctgct cctcctggtc
     6481 gggctgctgg ccccgacggg gctgcaggcg cgcggggcag tggccgaccg ctacgaggcg
     6541 acggtgtcga tccggccgat cggcagcgtc ggccgattca ccgaggacgt cgccggagac
     6601 gcccggggcg ccacggcgtc gacctacggc ggcggcggcg agctcggcct gagctacggc
     6661 gtgcggaact ggctcgacct cggcgccgag ctcgcggcgg cggggttcac gcgggccacc
     6721 taccgcgacg cgatcgtgac gatcatgggg atcccggcga ccggccgggt cacgcgcacg
     6781 gcccgcctga gccagctgcg cgcgggcgcg accttccggc tcgggctggc ctgggtgccg
     6841 acggtgtatg tcggcgcggg gctcagc
//
LOCUS       DASVOR010001647         4813 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_279073,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001647 DASVOR010000000
VERSION     DASVOR010001647.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4813)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4813)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4813
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279073"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VF516_30850"
     CDS             complement(<1..1106)
                     /locus_tag="VF516_30850"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8112187.1"
                     /translation="MGSSHLDWVPPENFDEYRLIKALGRGSMGQVWLAHDTVLDRLVA
                     VKFIAELPVHDASRQRFLTEARAAARVQHPNIIAAYRVGEIGARPYLISEYVRGDSLD
                     TIARPVAWPQLLEIAIGLARGLAAAHRQGVLHRDLKPANAILSEAGEVKLLDFGLAKL
                     LPQVAQPQVAQPRADAPPGSEAAAPLPDVPDPPSTPPRASFAPAETPPDRHTKTLELP
                     ASAPAGTPGGGIALPSVTTTGAVMGTPAYMPPEAWRGEPATPRSDVYSLGAMLHELCV
                     GAPPHRGDSPELTWGSVLAGEAPPLAAVAPGIDPGFAAIVDRCLRRDPTERFASGDAV
                     REALEALAARSAHELAAPRDRETRSDGPPASAVAA"
     gene            1301..3172
                     /locus_tag="VF516_30855"
     CDS             1301..3172
                     /locus_tag="VF516_30855"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8112188.1"
                     /translation="MCEGRGPYTRMRIMPVDSNASTYRPDDAAMSRLDPSAPDVFESA
                     YLLVLEGASSRAENLPLDGTVVIGRADGAQIRLVDKSVSRAHARISMHGGRAEIVDLG
                     SQNGTKVNGERIVGARPLLSGDVIAIHSVTLVFHSSAPVESRRQLLALDGFRQRVEDE
                     FDRLARNRRAFGVAAIAFVPPDSDAPPRAFDPIAVAQLVEPALRRIDVIALNGAELLV
                     LMPEVDAAMAQQLTRGLLDLLCVDAPRACGGVALAPADGNDFEILLSGARAARGGAGP
                     GKVGLAAETYRVIEIGGSHLVVADPAMLRIVALLERLSKSDLSVLVCGETGTGKELAA
                     TVLHQGSVRAARPLVVLNCAALPEALAESELFGHEKGAFTGAVAQKIGAFEQSDGGTV
                     FLDEIGELSLAIQAKLLRALETKRITRVGGHGEHPIDLRIVAATNRNLADEVKAGRFR
                     QDLLFRIGGATVWLPPLRDRRREIPILAQRFLADACRRAGRDAMAISVEAMQLLLDYA
                     WPGNVRELRHVMEYVAAAHDEPSVAAWHLVDRLGGEGRPRRSRAVTVTMPAAPGSPGG
                     DFAPIEDEVRELERTRMSQALAAADGNQTAAAELIKMPLRTFQAKAKVYGLRHKDRR"
     gene            3505..>4813
                     /locus_tag="VF516_30860"
     CDS             3505..>4813
                     /locus_tag="VF516_30860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112189.1"
                     /translation="MTAQWLGDYEILQRLRIGGMATLYLARRHGAAGFSRLVALKMIH
                     PHLAEQASFVDMFVDEARICAQISHPHVVHVEEFGVLDGVHYLVMEYIDGCSAFELLQ
                     VFYRERRMLDPELVARVIMQVAGGLHAAHETGGPDGQPLDLIHRDISPSNILVSVDGN
                     AKLIDFGIAKARNRVSETQAGFTLKGKCRYVAPEQALLAEVDRRCDIFSLGIVFWELL
                     VGRQLFPDDSHTALVNRLHRTDVPAPSAVNPGVPAVFDPVVLAMLQHDPADRPQTAAE
                     VQRRIASAIPGAANREAAELGALAIEVRDKCAQRRARRASAESPSSDSDRNFSPIRSP
                     RPRTSVDHRVDLEASPRSGVQDASPPAPPPWWRRRGVQLGASAAGVALLLVVLLGHRG
                     SEAAAPRPPRERELVEWREPVTRVESPPLRPTMPAPPVVVAASR"
BASE COUNT          662 a         1711 c         1750 g          690 t
ORIGIN      
        1 gccgccacgg ccgacgccgg cgggccgtcg cttcgggtct cgcgatcgcg gggcgcggcg
       61 agctcgtgcg ccgatcgcgc ggcgagcgct tcgagcgcct cgcgcacggc gtcccccgac
      121 gcgaaccgtt cggtcggatc gcgccggagg cagcgatcga cgatggcggc gaagccggga
      181 tcgatcccgg gcgcgacggc cgccagcggc ggcgcttccc cggcgagcac ggacccccag
      241 gtgagctccg gcgagtcgcc gcggtggggc ggagcgccga cgcagagctc gtggagcatc
      301 gcgccgaggg agtagacatc cgagcgcggc gtcgccggct cgccgcgcca cgcttcgggc
      361 ggcatgtacg ccggtgtgcc catcaccgcg ccggtcgtcg tgaccgaagg caacgcgatg
      421 ccgccgccgg gggtgcctgc gggcgcgctg gccggtagct cgagggtctt ggtgtgccga
      481 tccggcggcg tttcggccgg tgcgaagctc gctcgcggcg gggtggacgg agggtccgga
      541 acgtccggaa gcggcgccgc tgcctcgctg cccggtggcg catccgcgcg cggctgcgcg
      601 acctgcggct gcgcgacctg cggcagcagc ttggcgagcc cgaaatcgag cagcttgacc
      661 tcgcccgcct ccgacaggat cgcgttggcc ggcttgaggt cacggtgcag cacgccctgg
      721 cggtgcgccg cggcgagccc gcgtgcaagg ccgatcgcga tctcgaggag ctgcggccac
      781 gcgaccggcc gcgcgatcgt atcgaggctg tcgccccgga cgtactccga gatcaggtac
      841 ggtcgcgcac cgatctctcc gacgcggtac gccgcaatga tgttcgggtg ttgcacgcgc
      901 gctgccgcac gagcttccgt gaggaatcgc tggcgggagg cgtcgtgtac cgggagctcg
      961 gcgatgaact tcaccgcgac cagacggtcg agcacggtgt cgtgggcgag ccagacctgc
     1021 cccatgctgc ctcggccgag cgctttgatg agccggtact cgtcgaagtt ttccggaggc
     1081 acccagtcca gatggctcga acccatctgg ggtcgattga agcacgggcc gaaacccctg
     1141 aggagcgtat cgacgcgcgc ctccctcggc gtccgctcca ccggatccag gtggcgctgc
     1201 attttgcggc atgtgtgagg caaaccgatg gatggggccc ctacgatctg tgacacttgc
     1261 cacggcatgg cggtccgcga ccgggccgtg cggtccaggg atgtgcgagg gacggggtcc
     1321 gtatactcgt atgcggatca tgcccgtaga ctcgaatgcc tccacgtacc ggccggacga
     1381 cgcggcgatg tcccggctcg atccgtcggc gcccgatgtc ttcgagagcg cgtacctcct
     1441 cgtgctcgag ggcgcgtcgt cgcgcgcgga gaacctgccg ctggacggca cggtcgtgat
     1501 cgggcgcgcg gacggcgccc agatccgcct cgtcgacaag tcggtgtcgc gcgcccacgc
     1561 gcggatctcg atgcatgggg ggcgggccga gatcgtcgat ctcggcagtc agaacggcac
     1621 caaggtcaac ggcgagcgga tcgtcggtgc ccggccgctg ctgtcgggcg acgtcatcgc
     1681 gatccacagc gtcacgctgg tgtttcactc gtcggcgccg gtcgagtcgc gccggcagct
     1741 gctcgcgctc gatggctttc gccagcgggt cgaggacgag ttcgaccgcc tcgcgcgcaa
     1801 ccgccgggcc ttcggcgtgg ctgcgatcgc gttcgtcccg cccgactccg atgctccgcc
     1861 acgcgcgttc gatccgatcg cggtggcgca gctcgtcgag ccggcgctgc gccgcatcga
     1921 cgtgatcgcg ctcaacggcg ccgagctcct cgtgctgatg ccggaggtcg acgccgccat
     1981 ggcgcagcag ctcacgcgcg ggctgctcga cctgctgtgc gtggacgcac cgcgcgcgtg
     2041 cggcggcgtc gcgctggcgc cggccgacgg gaacgacttc gagatcctgc tgagcggcgc
     2101 ccgcgccgcc cgtgggggag ccgggccggg caaggtcggg cttgccgccg agacctaccg
     2161 ggtgatcgag atcggcggca gccacctggt cgtcgccgat cccgcgatgc tgcgcatcgt
     2221 ggcgctgctc gagcgactgt cgaagtcgga cctctccgtg ctggtatgcg gcgagaccgg
     2281 gaccggcaag gagctggccg cgacggtcct gcaccagggg tcggtccgcg cggcccgtcc
     2341 gctggtcgtc ctcaactgcg ccgcgctgcc cgaggcgctc gccgagagcg agctgttcgg
     2401 gcacgagaag ggcgcgttca ccggggcggt cgcgcagaag atcggcgcgt tcgagcagag
     2461 cgacggcggc accgtgttcc tcgacgagat cggcgagctg tcgctcgcga tccaggccaa
     2521 gctgctccgc gccctcgaga ccaagcggat cacgcgcgtc ggcggccacg gcgagcaccc
     2581 gatcgacctg cgcatcgtcg ccgcgaccaa ccgcaacctc gccgacgagg tcaaggccgg
     2641 ccggttccgc caggatctgc tgttccggat cggcggcgcg accgtgtggc tgccgccgct
     2701 ccgcgaccgc cgccgcgaga tcccgatcct cgcgcagcgc ttcctcgcgg acgcctgcag
     2761 gcgcgccggg cgcgacgcga tggcgatctc cgtggaggcg atgcagctcc tgctcgacta
     2821 cgcgtggccg ggcaacgtgc gcgagctgcg ccacgtcatg gagtacgtcg ccgcggccca
     2881 cgacgagccg agcgttgccg cctggcacct cgtggaccgg ctcggcggcg aaggccggcc
     2941 gcgccgcagc cgcgcggtca cggtcaccat gccggcggcg cctgggtcgc ccggcggaga
     3001 cttcgcgccg atcgaggacg aggtccgcga gctcgagcgg acccgcatga gccaggcgct
     3061 ggcggcggcg gatggcaacc agaccgcagc cgccgagctg atcaagatgc cgctgcgtac
     3121 gtttcaggcc aaggcaaagg tgtacgggct acgacacaag gaccgtcgct gacggtaccg
     3181 gacgccccgc agggcccgcg gtgcccgaca ccgcaacttc tgcggtcccg caggatctgc
     3241 gcttcgcgac accgcaactt ctgcatcgag ccgatcctgc ggttcgacgc cggcggtctc
     3301 ctccagcggt cttgctgagc ggtttatcga ccacgatcgc ggcaaaccgc gacgaggccg
     3361 atccctgccg gcggtgtggt tgcggccgtg cgcgccgggc atggcgagtg caaatgcaag
     3421 ccactcgaca ccggcgctga cacggcgcgc gtcgccgcgg gtcatcggtg cgtcgactga
     3481 catctgcgac acggacggag ttctatgacg gcgcaatggc tcggcgacta cgagatcctc
     3541 cagcggctgc gcatcggcgg aatggcgacg ctctacctcg cgcgccgcca cggcgcggcg
     3601 ggattctccc ggctcgtcgc gctcaaaatg atccatccgc acctggccga gcaggcgagc
     3661 ttcgtcgaca tgttcgtcga cgaggcccgg atctgcgcgc agatcagcca tccccacgtc
     3721 gtccacgtcg aggagttcgg tgtgctcgac ggggtccact acctggtgat ggagtacatc
     3781 gacggctgct cggcgttcga gctgctgcag gtgttctacc gcgagcgccg catgctcgat
     3841 cccgagctgg tggcgcgcgt gatcatgcag gtcgccggtg ggctccacgc cgcgcacgag
     3901 accggtggcc ccgatggcca gccgctcgac ctgatccatc gcgacatcag cccgtcgaac
     3961 atcctcgtct cggtggacgg caacgcgaag ctgatcgatt tcgggatcgc caaggcgcgc
     4021 aaccgggtct ccgagaccca ggcgggcttc acgctcaagg gcaagtgccg ctacgtggcg
     4081 cccgagcagg cgctgctcgc ggaggtcgac cggcgctgcg acatcttctc gctcggcatc
     4141 gtgttctggg agctgctggt cggccggcag ctgttccccg acgacagcca caccgcactg
     4201 gtcaaccggc tgcaccggac cgacgtcccg gcgccgagtg cggtgaaccc cggcgtgccg
     4261 gcggtgttcg atccggtcgt gctggcgatg ctgcagcacg atccggcgga ccggccgcag
     4321 accgccgccg aggtccagcg ccggatcgcg agcgcgatcc cgggcgccgc caaccgtgag
     4381 gcggccgagc tgggcgcgct cgccatcgag gttcgcgaca agtgcgcaca gcggcgcgcc
     4441 cggcgggcga gcgccgaatc acccagctcg gacagcgacc ggaacttctc gccgatccgc
     4501 agcccccgcc cgcggacatc cgtcgaccat cgtgtggacc tcgaggcctc ccctcgctcc
     4561 ggcgtacagg acgcatcgcc gccggctccg ccgccgtggt ggaggcggcg cggtgtacag
     4621 ctcggcgcca gcgcggccgg cgtagctctg ctgctggtcg tcttgctcgg acaccggggg
     4681 agcgaagccg cggcgccgag gcctccgcgc gagcgcgagc tggtcgaatg gcgcgagccg
     4741 gtcacgcggg tggaatcgcc accgctgcgc cccaccatgc cggcgccgcc cgtggtggtg
     4801 gcggcgtcca ggc
//
LOCUS       DASVOR010001648         3623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_192550,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001648 DASVOR010000000
VERSION     DASVOR010001648.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192550"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="VF516_30865"
     CDS             <1..288
                     /locus_tag="VF516_30865"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8112190.1"
                     /translation="LRAALGGLDARARALLRYQLIDGWSIDQVGKLYGVHRATAARWL
                     ADARAALGDAIRGELAARLRIAASEVDSIVRLVQSRVDMSLDRLLVTDASR"
     gene            562..2238
                     /locus_tag="VF516_30870"
     CDS             562..2238
                     /locus_tag="VF516_30870"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112191.1"
                     /translation="MPTTLTPTDLQIGDYEPLRITYGRDILVVDDNQTNLIAIEAALS
                     PLGRKLVLAHSGVEALGRLLEQDFALIILDVAMPGMDGFETAKLVRSRERNRATPIIF
                     ITGLSWENDAVLRGYELGAFDFLMKPIRPEVLRAKATVFVQLQERTIELAVKADELRQ
                     AQIRAYERELHAQRRRFEAEVMERQMQQLAEADRRKDEFLAILAHELRNPLQPLQTAV
                     EVLEHEPDKPVPARIRKIVQRQVQHITRLVDDLLDVARFTAGKLELRREPIDLDGIVD
                     EAVASCRTSIDSRNHHLEVSAHAAPAIVHGDPVRLIQCVCNLIHNAVKYTEPGGRLTI
                     DWGSERGDGFVRVTDNGRGIPADLLPKIFDMFVQERVTPDGGGGLGLGLGLVKRLIEL
                     HGGTVRATSEGQGRGATFEIRMPLVDQATLEKLKPMRNKTGPQDRPLRAVVCDDATDL
                     RDLVADLLRLRGHEVSVVGDGPAAIQLIVAERPDVALIDIGLPDMDGYEVARTIREQL
                     ADHKPRLIAMTGYGQASDRAAAFEAGFDAHIVKPASADKILRALYGTEEA"
     gene            2235..>3623
                     /locus_tag="VF516_30875"
     CDS             2235..>3623
                     /locus_tag="VF516_30875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8112192.1"
                     /translation="MNTFELPRNGRNGRNGRSGKNGNGNGNGNGKPQRTASAAVERTP
                     VSPGLEHRQILSALRAFKRGDFTVKIREDLIGVDGQIAETFNEIVEMVRTIRDEANDV
                     SGAVGKQGQAGKRMRRFNLSGGWADYVQSVNEVINDLTGHANEIARVVTAVARGDLEE
                     TMDADTPEGPRRGEFLRHARIVNGMVARLAQFGSEVTRVAQEVGGEGKLGAQARVQGV
                     SGVWKDLTDSVNLMASNLTSQVREIARVTTAVAQGDLTKTITIDVKGEILELKNTMNT
                     MVEQLRAFANEVTRVAREVGTEGRLGGQATVRGVSGVWRELTESVNSMANNLTQQVRN
                     IAEVTSAVASGDLGKKITVEAKGEILSLKNTINIMVDQLSSFAAEVTRVAREVGTEGT
                     LGVTAEVADVSGVWRELTQNVNSMASNLTSQVRAIAEVTTAIADGDLSKKIGVDARGE
                     ILALKNTINTTVD"
BASE COUNT          627 a         1276 c         1251 g          469 t
ORIGIN      
        1 ctgcgcgccg cgctgggagg gctcgacgcg cgggcgcgcg cgctgctgcg ctaccagctg
       61 atcgacggct ggagcatcga ccaggtcggc aagctgtacg gcgtgcatcg cgcgaccgcg
      121 gcgcgctggc tcgccgacgc ccgcgccgcg ctgggcgacg cgatccgcgg cgagctcgcg
      181 gcgcggctcc ggatcgccgc cagcgaggtc gattcgatcg tgcggctggt ccagagccgg
      241 gtcgacatgt cgctcgatcg cctgctggtc accgacgcgt cccgctagcg cacaccgtgt
      301 tggcgggcac cagcgcgcac cgagcgtctg agcacaccga gcgccatcgc acagcacact
      361 cgatgaaagc ctttagaaac aatgggtccg atcacacggg cggctgggca cgctgcgtgc
      421 acaataccgc ccggcggcgg tcattcccat gggtcatgcg ttcacagccg ccggggaagg
      481 gactgcgcaa gttgtgcctc gcgtgatgtg cgtgctcgat ccacggtgtc gccggaacgg
      541 ccaacgtccc ttcacccttc catgccgacg acgctgacac cgaccgatct gcagatcggg
      601 gactacgagc ccctgcggat cacctacggc cgtgacatcc tggtcgtcga cgacaaccag
      661 accaacctga tcgcgatcga ggccgcgctg tcgccgctgg gccgcaagct cgtgctcgcg
      721 cactccggag tcgaggcgct cggccggctg ctcgagcagg acttcgcgct gatcatcctc
      781 gacgtcgcga tgccgggcat ggacgggttc gagaccgcca agctggtccg ctcccgcgag
      841 cgcaaccgcg cgacgccgat catcttcatc accgggctgt cgtgggagaa cgacgccgtg
      901 ctgcgcggct acgagctcgg cgcgttcgac ttcctgatga agccgatccg gcccgaggtg
      961 ctgcgcgcca aggccaccgt gttcgtccag ctccaggagc gcacgatcga gctcgcggtc
     1021 aaggccgacg agctccgcca ggcccagatc cgcgcctacg agcgcgagct ccacgcccag
     1081 cgccgtcggt tcgaggccga ggtcatggag cgccagatgc agcagctcgc cgaggccgac
     1141 cgccgcaagg acgagtttct cgcgatcctc gcccacgagc tgcgcaaccc gctgcagccg
     1201 ctgcagaccg cggtcgaggt cctcgagcac gagcccgaca agccggtgcc ggcgcggatc
     1261 cgcaagatcg tgcagcgcca ggtccagcac atcacccgcc tggtcgatga cctgctcgac
     1321 gtcgcccggt tcaccgccgg caagctcgag ctccgccgcg agccgatcga cctcgacggc
     1381 atcgtcgacg aggcggtcgc gtcgtgccgg acctcgatcg acagccgcaa ccatcacctc
     1441 gaggtctcgg cgcacgccgc gccggcgatc gtccacggcg atccggtgcg gctgatccag
     1501 tgcgtgtgca acctgatcca caacgcggtc aagtacaccg agccgggcgg ccggctgacc
     1561 atcgactggg gcagcgagcg cggcgacggc ttcgtgcggg tcaccgacaa cggccgcggc
     1621 atccccgcgg acctgctgcc caagatcttc gacatgttcg tgcaggagcg ggtcaccccg
     1681 gacggcggcg gcgggctcgg gctgggactg ggcctggtca agcggctgat cgagctgcac
     1741 ggcggcaccg tgcgcgcgac cagcgagggc cagggacggg gcgccacgtt cgagatccgg
     1801 atgccgctcg tcgatcaggc aaccctggag aagttgaagc ctatgcgcaa caagaccggc
     1861 ccgcaggacc gacccctgcg cgcggtagta tgtgacgacg ccaccgacct gcgcgatctg
     1921 gtcgccgatc tgctgcggct gcgcggccac gaggtcagcg tggtcggcga cggcccggcg
     1981 gcgatccagc tgatcgtcgc ggagcgaccc gacgtggcgc tgatcgacat cgggctgccc
     2041 gacatggacg gctacgaggt cgcgcgtacg atccgcgagc agctcgccga ccacaagccc
     2101 cgcctgatcg cgatgaccgg ctacggccag gccagcgatc gggcggccgc gttcgaggct
     2161 ggattcgacg cccacatcgt caagcccgcg agcgcggaca agatcctccg cgctctgtac
     2221 ggcaccgagg aagcatgaac acgttcgagt taccccgcaa cggacgcaac ggacgcaacg
     2281 gacggagcgg gaagaacggc aacggcaacg gcaacggcaa cggcaagccg caacgaacgg
     2341 ccagcgcggc cgtcgagcga acgccggtgt cgccggggct cgaacaccgc cagatcctgt
     2401 cggcgctgcg cgcgttcaag cgcggcgact tcacggtcaa gatccgcgag gacctgatcg
     2461 gcgtcgacgg ccagatcgcc gagacgttca acgagatcgt cgagatggtc cggacgatcc
     2521 gcgacgaggc caacgacgtg tccggcgcgg tcggcaagca gggccaggcc ggcaagcgga
     2581 tgcggcggtt caacctgtcg ggcggctggg ccgactacgt gcagtcggtc aacgaggtga
     2641 tcaacgacct caccggccac gccaacgaga tcgcgcgggt ggtcaccgcg gtcgcgcgcg
     2701 gcgacctcga ggagaccatg gacgccgaca ccccggaggg tccgcgccgc ggcgagttcc
     2761 tgcgccacgc ccggatcgtc aacggcatgg tcgcccggct cgcccagttc ggctccgagg
     2821 tgacccgcgt cgcccaggag gtcggcggcg agggcaagct cggcgcccag gcccgggtcc
     2881 agggcgtgtc cggcgtgtgg aaggacctga ccgactcggt gaacctgatg gcgtcgaacc
     2941 tgacctcgca ggtccgcgag atcgcccgcg tcaccaccgc ggtcgcgcag ggcgacctga
     3001 ccaagacgat cacgatcgac gtcaagggcg agatcctcga gctcaagaac acgatgaaca
     3061 cgatggtcga gcagctgcgc gcgttcgcca acgaggtgac gcgcgtcgcc cgcgaggtcg
     3121 gcaccgaggg ccggctcggc ggccaggcca cggtgcgcgg cgtgtccggc gtgtggcgcg
     3181 agctgaccga gtcggtcaac tcgatggcca acaacctgac ccagcaggtc cgcaacatcg
     3241 ccgaggtgac ctcggcggtc gcgtccggcg acctcggcaa gaagatcacg gtcgaggcca
     3301 agggcgagat cctgtcgctc aagaacacga tcaacatcat ggtcgaccag ctgagctcgt
     3361 tcgccgccga ggtcacccgg gtggcgcgcg aggtcggcac cgagggcacg ctcggcgtga
     3421 ccgccgaggt cgccgacgtg tccggcgtgt ggcgcgagct gacccagaac gtcaactcga
     3481 tggcgtcgaa cctgacctcg caggtccgcg cgatcgccga ggtcaccacc gcgatcgccg
     3541 acggcgacct cagcaagaag atcggggtcg acgcgcgcgg cgagatcctc gcgctcaaga
     3601 acacgatcaa caccacggtc gac
//
LOCUS       DASVOR010001649         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_192554,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001649 DASVOR010000000
VERSION     DASVOR010001649.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192554"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..901
                     /locus_tag="VF516_30880"
     CDS             161..901
                     /locus_tag="VF516_30880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112193.1"
                     /translation="MLTSIPQRFAWWFLGLGLAPVIAHAGPAEDAKAHVARASQAHKE
                     GRYDDARVELEAAYALTPKPELLYALGQVHAKLGRCGDATTYFQRFAAAQHDPQVAKV
                     IEQAIASCKPAAPPPAASTQEPPPASPAPPAVASPEREAWYHDKLGDGLVLAGATAVI
                     VGLVEYRSAVSDLDAAEASSTATRYLQLVGEAHDKRTTALVLTGVGSALIAGGLVHYA
                     LRDRGPEARVVAVAPAPGGGLVTYQGRF"
     gene            901..1506
                     /locus_tag="VF516_30885"
     CDS             901..1506
                     /locus_tag="VF516_30885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX8112194.1"
                     /translation="MRWAAIGLAAVAVTGCLRDTEFKCTQDSACEAMGHCEAVGFCSV
                     VDAQCTTGRRFSETAGQGLASTCVPATAGNVGRCPSDYVAVGSSGHRYKRLGTVSWDQ
                     ARAMCEQASPGTYLLIPDDATELADLATIASPPFWIGVDDLAAPGTFMTVKSVRAMFT
                     PWATGEPSAQLGRDCVRAVSATQIATDLCSNRRAAICECEP"
     gene            1933..>2428
                     /locus_tag="VF516_30890"
     CDS             1933..>2428
                     /locus_tag="VF516_30890"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8112195.1"
                     /translation="MTTDHILQTQGLTKDFRGFVAVKSVDLRVRRHSVHALIGPNGAG
                     KTTCFNLLTKFLTPSAGTITYRGIDITHWKPAQVTQLGLVRSFQISAVFPHLTVLENV
                     RIALQRKRGRSFDFWRSQAVLAGFEPRARELLDAVGLGAFAGQRAVELSYGRKRALEI
                     ATTLA"
BASE COUNT          330 a          911 c          858 g          329 t
ORIGIN      
        1 ccgcgcccac cggagatctg ccatcggcgg cccggccgag cgcgtcgcac gaccccgcgc
       61 gcgcggccag accggcgagc ccgccgcgca ccgcaccggc caagccgctg gcctacgacc
      121 ccgacgcgct gttcctcccc aagcagtgac aggcgccgag atgctgacat cgatcccgca
      181 gcgattcgcg tggtggttcc tcggcctcgg cctcgcgccc gtgatcgcgc acgccggccc
      241 ggccgaggac gcgaaggcgc acgtggcgcg ggcgagccag gcgcacaagg aggggcgcta
      301 cgacgacgcg cgcgtcgagc tcgaggcggc ctacgcgctg accccgaagc ccgagctgct
      361 ctacgcgctc ggtcaggtcc acgccaagct cggccgctgc ggcgacgcga ccacgtactt
      421 ccagcggttc gccgccgcgc agcacgatcc gcaggtcgcc aaggtcatcg agcaggcgat
      481 cgcatcgtgc aagccagcgg ccccgccacc ggctgcatcg acccaggagc caccgccggc
      541 gagtcctgcg cccccggcgg tcgcgtcgcc cgagcgcgag gcctggtacc acgacaagct
      601 cggcgacggc ctggtgctcg ccggcgccac cgccgtgatc gtcgggctgg tcgagtaccg
      661 cagcgcggtg tccgatctcg acgccgccga ggcctcatcc acggccactc gctatctcca
      721 gctcgtcggc gaggcgcacg acaagcggac gaccgcgctc gtgctcaccg gcgtcggtag
      781 cgcgctgatc gccggcggcc tcgtgcacta cgcgctgcgc gatcgcggcc cggaggcgcg
      841 cgtcgtcgcg gtcgcgccgg cgccgggcgg cggcctggtc acctaccagg ggcggttctg
      901 atgcggtggg cggcgatcgg cctcgcggcg gtggcggtca ccggctgtct gcgcgatacc
      961 gagttcaagt gcacgcagga ttccgcctgc gaggccatgg gccactgcga agcggtcggc
     1021 ttctgcagcg tcgtcgacgc ccagtgcacc acgggccggc ggttcagcga gaccgccggt
     1081 caggggctgg ccagcacgtg cgtgcccgcg accgcgggga acgtcggccg gtgtccgtcg
     1141 gactacgtcg cggtcggcag ctccggtcac cgctacaagc ggctcggcac ggtgtcgtgg
     1201 gaccaggcgc gcgcgatgtg cgagcaggcc agccccggga cctacctgct gattcccgac
     1261 gacgccaccg agctcgccga cctggcgacg atcgccagcc cgccgttctg gatcggcgtc
     1321 gacgacctgg ccgccccggg cacgttcatg accgtcaaga gcgtccgggc gatgttcacg
     1381 ccgtgggcga ccggcgagcc gagcgcgcag ctgggcagag actgcgtccg tgcggtctcc
     1441 gcgacgcaga tcgcgaccga cctgtgcagc aaccggcgcg cggcgatctg cgaatgcgag
     1501 ccgtgacgcc ggagatcgcg gccggtcatc ggttcgatgg gcagcgttcg agcgcgcagt
     1561 cagacaccgc gtgccccgcg tgagggggta ggttcccgcc cacgccaatc accttcctcg
     1621 tcgtgccgca gtggcagggc tcggggtcgc cgtgccggct gtcgatccgg cagatcctcg
     1681 gcgcgctgac cggctgaccg gcccgcggac agcgtcagcg ctgacgcatc tggtcaccgc
     1741 ggggagcgtc ggagatgacg cacctcgcat gccgcttcac gggtctgccc cccgccccgg
     1801 ctggcgtgca ccctgcagtc gccgcggacc ccaaatgccc cggatgacgc tcccaccgct
     1861 tggtttgctg tatgcggaca gggtaccgtg cggtgcggcg tacggcgggc gtctgtccag
     1921 gcggctttgg cgatgaccac cgaccacatc ctccagaccc agggcctcac caaggacttc
     1981 cgcggcttcg tcgcggtcaa gtcggtcgac ctgcgggtcc gccggcactc ggtgcacgcg
     2041 ctgatcggcc ccaacggcgc gggcaagacg acctgcttca acctgctgac caagttcctg
     2101 accccgtcgg cgggaacgat cacctaccgc ggcatcgaca tcacgcactg gaagcccgcg
     2161 caggtcaccc agctcgggct ggtgcgatcg ttccagatct cggcggtgtt cccgcacctc
     2221 accgtgctgg agaacgtgcg gatcgcgctg cagcgcaagc gcggccggtc gttcgacttc
     2281 tggcggtccc aggcggtgct cgccgggttc gagccgcgcg cccgcgagct gctcgacgcg
     2341 gtcgggctcg gcgcgttcgc cggccagcgc gcggtcgagc tgtcgtacgg ccgcaagcgc
     2401 gcgctcgaga tcgcgaccac gctggcgc
//
LOCUS       DASVOR010001650         7008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_75221,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001650 DASVOR010000000
VERSION     DASVOR010001650.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7008
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75221"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2908)
                     /locus_tag="VF516_30895"
     CDS             complement(<1..2908)
                     /locus_tag="VF516_30895"
                     /inference="COORDINATES: protein motif:HMM:NF036537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LodA/GoxA family CTQ-dependent oxidase"
                     /protein_id="HEX8112196.1"
                     /translation="MATLDKVQIFPAIGIARLGNSQSWFLGPELPFPAAPPPPPGGTY
                     KDEACRIKRQAQRFRLWGFFSDGSDRELTLADGDITWTVHLVNAKPSYRYAGEDTIDG
                     GAHTLAGAGATAAFAGTFAGVTVPLGDAETDGEGRLIVRGGFGKSENPTDPSSLPSFP
                     STPGWYDDVSDGPITAQITIGGNTFTAEHGAWVICPPPRYAPTTYSITTLYDTLRQLA
                     LDHGQLPPLGPPSFAADVYPILQRALDMRYVTAFAFGAGDHDSLTAFIPPGGPGDTLA
                     NRQMVYQKLRPSGDMPLLFDGGGTTSQLRAFQLGLIQQWSQGQGTADWPPVVPTAISP
                     DGLTRAALEACVGAAFFPGIEAGGTPSSVQITTLAFAEPFRLDPGSVQPGDVTKGMAL
                     PWQGDFMLCSGPESDAGNDQSSWWPAARPIGIYPIDDPSTKRLWTLGVASSMQDMVDN
                     WHELGFIVDKGLGKPVETEKTNVCKSCFFINNRPEIGKDEAQSLIDSGQHVEDAFYVV
                     VQGLAPSDLMITTPNPSQSQLAAWAPQIANPVPGTMTITAFDLALEDAGHLGQVQRIT
                     FGYNLTFTSTSAFTAEDVPVTLTASVLGLSSTTILDLTTRQHPYMVAGATSWLSADTR
                     VFKLQPGGMFAHRTLQNDPSAFIEAVIDSLRGSQQANPWFDNLPADEAGAQLEWSQTL
                     DGQPVYNFAICRVRYRAELNPAPDVRVFFRLFPAMTTSTDFQPATTYRTGGQPGTKIP
                     LLGIVGGEVTTIPFFAAPRRPATDNLNLQQDPKNIDTLQVNAGGNETYSFFGCWLDIN
                     QPGDKRFPIQPTPPDGPFTGPAPLQSIADLIRGTHQCLVAEIHFDPDPIAVGATTASS
                     DKLAQRNLSIDHSDNPGEPDTHRVQHTFTIRPTTPSPQPRQGPDELMIDWGTTPHGTK
                     ATLYLPGVSASEVLRLADRLYTRNRLTAVDVHSVQIEVTGGVSYVPVPSGGVGDL"
     gene            complement(2916..3929)
                     /locus_tag="VF516_30900"
     CDS             complement(2916..3929)
                     /locus_tag="VF516_30900"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8112197.1"
                     /translation="MWASTEHEVCVFGAGPAGLAVAAQLLQHGRDVCVLDRPARARPW
                     GGESFSGAIRGPMIALGFWDRFERAAHLQGFERQSAWGGEPWAESTVFRPGGALWHVD
                     RDRFDDDLRAAVRERADIVTPYRRLDAVRREAGKWRVALDGTTEVVARYLVDATGRLR
                     ALGHRLGGRIETHDRLLGLTSRVRWDGAPEIRSMMLEATPFGWWYAAPTPGGHVVALF
                     TDADLAPPDVRRRLRTVAAHSAFMEVAADEGWQPVGDACASHDPLTGWGVHRALSNGL
                     VLADAITALLARGDGSPLEDYRQLCRQQYRSYLAGLVRHYSIERRWPSAPFWQRRHRL
                     ASA"
     gene            4439..5467
                     /locus_tag="VF516_30905"
     CDS             4439..5467
                     /locus_tag="VF516_30905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112198.1"
                     /translation="MTRICLSFISTLAAVALTAASANAALISGPRSIGSFYTSDDGAS
                     HAIIGKSSGDVVEAYWWEGTGTSASVIAHFDSIAAVSGFWANDSWRHAVVALHNGQVW
                     DVRYHPCCGIFPTMLVNLAGWGDIKSMTAWTDPQSHTNIAFLTHWGTGNAFGVFQTGG
                     NTPTTTTFVNIYQNDSAIDVAGHHEIWNATNMVSVAVGNPSHVEQIYWNDNQGPGNFF
                     DISPGTNVPWATRFSGAAVVSLSATDAFCYFCSQWAGVEQMDMVTRTNQMLTFDVNNG
                     GGLLFADWTFTYGPAVQSITGPFTSPVSGGFRRNTLVAMTNGDIWDMNPLWTSGAVSW
                     TFRFVGNF"
     gene            5617..6873
                     /locus_tag="VF516_30910"
     CDS             5617..6873
                     /locus_tag="VF516_30910"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8112199.1"
                     /translation="MTAEVAPGCPVDAAFDPLSPAYLDDPYAILAALPLAEAPIFYAP
                     SIGYHVVTRYADIAQVFDDPATYSAAVAQAPLVPLSPEAQRILLDGGHAPQPSMVSLD
                     EPAHGRLRKPAARAFAVKRVNDLVPTIRRIAGELLDGVTAAGAGFDLIGELAFPLPAS
                     IVFSLIGVPEADVPQLKRWCGHRAALGWGRPRPEDQVEIATHMAAYRRYLRELVERKR
                     AARGDDLTSDLLAIHDEDPERLTLAEIASILFSLSFAGHETTTGLIGNTVRRLLEAPE
                     RWDALVREPALIPAAVDESLRFDPSVPVWRRVTTRPVTLAGVELPRGARLFLWLAAAN
                     RDASVFPDPDRFDLHRPNADQHLAFGRGLHYCLGAHLGKVEARIVLDELTRRFPRLRL
                     VPGQRLSFHPNISFRGPQQLRVRAAP"
BASE COUNT          984 a         2454 c         2524 g         1046 t
ORIGIN      
        1 cgaggtcgcc gaccccaccg gacggcaccg gcacgtagct gacgccgccg gtgacctcga
       61 tctgcacgct gtggacgtcg accgcggtga gccggttccg ggtgtacagc cggtcggcga
      121 gccgcagcac ctccgacgcg ctgacgcccg gcaggtacag cgtggccttc gtcccgtgcg
      181 gtgtggtccc ccagtcgatc atcagctcgt ccggcccctg ccgcggctgc gggctgggcg
      241 tggtcgggcg gatcgtgaag gtgtgctgca cccggtgcgt gtccggctcg cccgggttgt
      301 ccgagtgatc gatcgacagg ttgcgctgcg ccagcttgtc ggacgatgcc gtggtcgcgc
      361 cgaccgcgat cggatcggga tcgaagtgga tctccgcgac caggcactga tgggtgccgc
      421 ggatcaggtc cgcgatcgac tggagcggcg cgggcccggt gaacggaccg tcgggcggcg
      481 tcggctggat cgggaagcgc ttgtcgccgg gctggttgat gtcgagccag cagccgaaga
      541 agctgtaggt ctcgttgccg ccggcgttga cctggagcgt gtcgatgttc ttcggatcct
      601 gctgcaggtt caggttgtcg gtcgccggcc ggcgcggcgc ggcgaagaac gggatcgtgg
      661 tgacctcacc gccgacgata ccgagcagcg ggatcttggt cccgggctgg ccgccggtcc
      721 ggtacgtggt ggccggctgg aagtcggtgc tggtggtcat cgccgggaac aggcggaaga
      781 acacgcgcac gtcgggggcc gggttcagct cggcccggta gcgcacccgg cagatcgcga
      841 agttgtagac cggctggccg tcgagcgtct gcgaccactc gagctgggcg ccggcctcgt
      901 cggcgggcag gttgtcgaac cagggattgg cctgctgcga gccgcgcagg ctgtcgatca
      961 ccgcctcgat gaacgcgctg ggatcgttct gcagcgtgcg atgcgcgaac atgccgccgg
     1021 gctggagctt gaacacccgg gtgtccgcgc tcagccacga cgtcgctccc gcgaccatgt
     1081 aggggtgctg gcgggtcgtc agatcgagga tcgtcgtcga cgacagtccg agcaccgacg
     1141 cggtcagcgt cacggggacg tcttcggccg tgaacgcgct ggtgctggtg aacgtcaggt
     1201 tgtacccgaa ggtgatccgc tgcacctggc ccaggtggcc agcgtcctcg agcgccaggt
     1261 cgaacgcggt gatcgtcatc gtgccgggga ccggattggc gatctgcggc gcccacgcgg
     1321 cgagctggct ctgcgaggga ttcggcgtcg tgatcatcag gtcggacggc gccaggccct
     1381 ggaccacgac gtagaacgca tcctcgacgt gctggccgga gtcgatcagg gactgcgcct
     1441 cgtccttgcc gatctcgggg cggttgttga tgaagaagca gctcttgcag acgttggtct
     1501 tctcggtctc gaccggcttg ccgagcccct tgtcgacgat gaacccgagc tcgtgccagt
     1561 tgtcgaccat gtcctgcatc gacgaggcga cgcccagcgt ccacagccgc ttggtcgatg
     1621 gatcgtcgat cgggtagatc ccgatcggac gcgccgccgg ccaccacgag ctctggtcgt
     1681 tgcccgcgtc ggactccggg cccgagcaca gcatgaagtc gccctgccac ggcagggcca
     1741 tccccttggt gacatcgccc ggctgcacgc tgcccggatc gagccggaac ggctcggcga
     1801 acgcgagcgt cgtgatctgg accgagctcg gggtgccgcc ggcctcgatc ccggggaaga
     1861 acgcggcgcc cacgcaggcc tccagcgcgg cgcgggtcag gccgtccggc gagatcgcgg
     1921 tcggaacgac gggaggccag tcggccgtgc cctggccctg gctccactgc tggatcaggc
     1981 cgagctggaa cgcgcgcagc tgcgaggtcg tgccgccgcc gtcgaacagc agcggcatgt
     2041 cgccgctggg ccgcagcttc tggtagacca tctggcggtt ggcgagcgtg tcgccggggc
     2101 cgccgggcgg gatgaacgcg gtgagcgaat cgtggtcgcc ggcgccgaac gcgaaggcgg
     2161 tcacgtagcg catgtccagc gcgcgctgga ggatcggata gacgtcggcg gcgaacgacg
     2221 gtggcccgag cggcggcagc tggccgtggt cgagcgcgag ctggcggagc gtgtcgtaca
     2281 gcgtcgtgat cgagtacgtc gtcggcgcgt agcgcggcgg gggacagatc acccacgccc
     2341 cgtgctcggc ggtgaacgtg ttgccgccga tcgtgatctg cgcggtgatc ggtccgtccg
     2401 agacgtcgtc gtaccagccc ggcgtgctcg ggaagctcgg cagcgagctc ggatcggtcg
     2461 ggttctcgct cttgccgaac ccgccgcgga cgatcaggcg gccctcgccg tcggtctcgg
     2521 cgtcgccgag cgggacggtc acgccggcga acgtgcccgc gaacgcggcc gtggcgcccg
     2581 cgccggccag ggtgtgcgcg ccgccgtcga tcgtgtcctc tccggcgtag cggtacgagg
     2641 gcttcgcgtt gaccaggtgg acggtccagg tgatgtcgcc gtcggccagc gtgagctcgc
     2701 gatcggagcc gtccgagaag aagccccaca ggcggaaacg ctgggcctgg cgcttgatgc
     2761 ggcacgcctc gtccttgtag gtgccgcccg gtgggggcgg cggcgccgcc gggaacggca
     2821 gctcggggcc gagaaaccag ctctggctgt tgccgagtcg ggcaatcccg atcgcgggaa
     2881 agatctgtac cttgtcgagc gtcgccatga atctcctatg cggatgcgag acggtgtcgt
     2941 cgttgccaga acggagcgct cggccaccgg cgctcgatcg agtagtggcg gaccaggccc
     3001 gcgaggtagc tgcggtactg ctgccggcag agctggcggt agtcctcgag cggtgagcca
     3061 tcgccgcgcg ccagcagcgc ggtgatcgcg tcggcgagga ccagcccgtt ggacagcgcg
     3121 cggtggacgc cccagccggt cagcggatcg tgggacgcgc aggcgtcgcc gaccggctgc
     3181 catccctcgt cggccgcgac ctccatgaac gcgctgtggg ccgcgaccgt gcgcagccgg
     3241 cgccggacgt cgggcggcgc gagatccgcg tcggtgaaca gcgcgacgac gtggccgccg
     3301 ggcgtcggcg cggcgtacca ccagccgaac ggcgtggcct ccagcatcat cgatcggatc
     3361 tcgggggcgc cgtcccagcg cacccgggag gtcaggccca gcaggcgatc gtgggtctcg
     3421 atccggccgc ccagccggtg gccgagcgcg cgcaagcgcc cggtggcgtc gaccaggtag
     3481 cgcgcgacca cctcggtcgt gccgtcgagc gccacccgcc acttgccggc ctcccgccgg
     3541 accgcatcga gccgccggta cggcgtcacg atgtccgcgc gctcgcggac ggccgcccgg
     3601 agatcgtcat cgaagcgatc gcggtcgacg tgccacagcg cgccgccggg acggaacacc
     3661 gtgctctcgg cccatggctc gccgccccac gccgactggc gctcgaagcc ctgcaggtgt
     3721 gccgcgcgct cgaaccggtc ccagaagccg agcgcgatca tcgggccgcg gatcgcgccg
     3781 gagaacgatt ctccgcccca cggcctcgcc cgcgccggcc ggtcgagcac gcacacgtcc
     3841 cggccgtgct ggagcagctg cgcggcgacc gccagccccg ccgggccggc gccgaagacg
     3901 cacacctcgt gctctgtgct cgcccacatc gtctcgcctc ctgcaccggg atgtccgccg
     3961 ccggtcccgc ctgtcgatga agacggggcc gggggacggc tctttcatga agaagtcctc
     4021 tcgcccgccg gtcgcacatc gcgatccgcg ggcaacgatt gccggtgcac cgccgcgagc
     4081 ggcggcagaa ccggccgccc gacagccgcg gcgcgaccgt gacttccatg tgacaccgcg
     4141 tccacgcgat cgtcgacggg aagttgcggc gacgttgcgg aagctttggc atcgtgccgc
     4201 acgggagcgg catgctggcg gtgatcccca cgatgtgcga gaggcgcccc cgatgaggga
     4261 gctgctgcac gctggacgcc acctccgcaa cacgtgacga cacggtggcg ccacgccgtg
     4321 gtccggcgat cgcgccaggc gtcggcaaac acgggatggt gccccccgcc ggcgcgggcg
     4381 cgcgcgttgc acggggcgac gcccgaacca acgccacaag gaggcgggaa tgtccacgtt
     4441 gactcgaatc tgtctgtcct tcatctcgac gctcgcggcc gtcgcgctca ccgcagcgtc
     4501 ggcaaacgct gccctgatca gcggaccgcg ctcgatcggc tcgttctaca cctcggatga
     4561 cggcgcgtcg cacgcgatca tcggcaagtc cagcggcgac gtcgtcgagg cctactggtg
     4621 ggaaggcacc gggacctcgg cgtcggtgat cgcccacttc gacagcatcg ccgcggtcag
     4681 cgggttctgg gcgaacgaca gctggcgcca cgccgtggtc gcgctgcaca acggccaggt
     4741 ctgggacgtg cgttaccatc cgtgctgcgg gatcttcccg acgatgctgg tcaacctggc
     4801 cggctggggc gacatcaagt cgatgacggc gtggaccgat ccgcagagcc acaccaacat
     4861 cgccttcctc acccactggg gcaccggcaa tgcgttcggc gtgttccaga ccggcggcaa
     4921 caccccgacc accacgacgt tcgtcaacat ctaccagaac gactcggcga tcgacgtcgc
     4981 cggccaccac gagatctgga acgcgacgaa catggtgtcg gtcgcggtcg gcaacccgag
     5041 ccacgtcgag cagatctact ggaacgacaa ccagggcccc ggcaacttct tcgatatctc
     5101 gcccggcacc aacgtgccgt gggcgacccg gttcagcggc gcggcggtcg tcagcctgtc
     5161 ggcgaccgat gcgttctgct acttctgctc gcagtgggcc ggcgtcgagc agatggacat
     5221 ggtgacgagg acgaaccaga tgctcacgtt cgacgtcaac aacggcggcg gtctgctgtt
     5281 tgccgactgg acgttcacct acggcccggc cgtgcagtcg atcaccggac cgttcacctc
     5341 gccggtgagc ggtggcttcc gccgcaacac cctggtcgcg atgaccaacg gcgatatctg
     5401 ggacatgaac cccctgtgga cctcgggggc ggtgtcgtgg acgttccggt tcgtcgggaa
     5461 cttctgacgc cggggcgctc gcgatccgcg cgccggccgg gctcggaacg gtgtgttttg
     5521 cctgcccggc gccgtgccgg cggtccatga tcggtcgcca ggagccgcga tgcgcagcca
     5581 ccgccccatt cgtcgccgag ccacgggagc tgcgcgatga ccgccgaggt cgcgccgggc
     5641 tgcccggtgg acgcggcgtt cgatccgctg tccccggcgt acctcgacga tccgtacgcg
     5701 atcctggccg cgctgccgct ggccgaggcg ccgatcttct acgcgccgtc gatcggttac
     5761 cacgtcgtca cccggtacgc cgacatcgcg caggtgttcg acgatccggc gacgtactcc
     5821 gcggcggtgg cccaggcgcc gctggtgccg ctatcgcccg aggcccagcg gatcttgctc
     5881 gacggcggcc acgcgccgca gccgtcgatg gtgagcctgg acgagccggc gcacggccgg
     5941 ctgcgcaagc cggccgcccg cgcgttcgcg gtcaagcggg tcaacgacct ggtgccgacg
     6001 atccgccgga tcgcgggcga gctgctcgac ggggtgacgg cggccggcgc cgggttcgac
     6061 ctgatcggcg agctcgcgtt cccgctgccc gcgtccatcg tgttctcgct gatcggggtc
     6121 ccggaggccg atgtcccgca gctcaagcgc tggtgcggcc accgcgctgc gctcggctgg
     6181 gggcggccgc ggcccgagga ccaggtcgag atcgccacgc acatggcggc gtaccggcgc
     6241 tacctgcgcg agctggtcga gcgcaagcgc gcggcgcgcg gcgatgacct gaccagcgac
     6301 ctgctggcga tccacgacga ggaccccgaa cggctcacgc tcgccgagat cgcctcgatc
     6361 ctgttctcgc tcagcttcgc cggtcacgag accacgaccg gcctgatcgg caacacggtg
     6421 cgccgcttgc tcgaggcgcc cgagcggtgg gacgcgctcg ttcgcgagcc ggcgctgatc
     6481 cccgccgcgg tcgacgagag cctgcggttc gatccgtcgg tcccggtgtg gcggcgggtc
     6541 accacgcgcc cggtcaccct ggcgggcgtc gagctgccgc gcggcgcccg gctgttcctg
     6601 tggctcgccg cggccaaccg cgacgcgtcg gtgtttccgg atccggaccg cttcgacctg
     6661 caccggccca acgccgacca gcacctggcg ttcggccggg gcctgcacta ctgcctgggc
     6721 gctcacctcg gcaaggtcga ggcccggatc gtgctcgacg agctcacccg ccgctttccg
     6781 cgcctgcggc tggtccccgg gcagcggctg agctttcacc ccaacatctc gtttcgcggg
     6841 ccgcagcagc tccgggtccg cgcggccccg tagcccagct cgccgccggc gatcggccat
     6901 ggcaagctgc gaccggggaa ggggaccatg accgaccaga tccggctcgc gacaccggac
     6961 gactacccgg cctacagcat cctgttccgc gagctcggcg tcgacgat
//
LOCUS       DASVOR010001651         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_338048,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001651 DASVOR010000000
VERSION     DASVOR010001651.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338048"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /gene="trpB"
                     /locus_tag="VF516_30915"
     CDS             complement(<1..869)
                     /gene="trpB"
                     /locus_tag="VF516_30915"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018036536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEX8112200.1"
                     /translation="MKTTTYVEIEEAEVSTTGTVHEIAALPAPDGGRFGGFGGQYVAE
                     TLMPAIAELEAAWRAARGDPSYWAEVEDLLVNYVGRPSLLYHARRLSEEVGAQVYLKR
                     EDLNHTGAHKINNCIGQAVLARKMGKRRLIAETGAGQHGVATATVAALFGLPCEIYMG
                     AEDVVRQALNVERMRLLGAQVHPVQSGTRTLKDAMNEALRDWVTNVGDTFYLIGSVAG
                     PHPYPWMVRDLQSVIGREARAQILSATGRLPDACVACVGGGSNAAGLFAPFAGDPGVR
                     LVGVEAAGEGIATG"
     gene            946..1224
                     /locus_tag="VF516_30920"
     CDS             946..1224
                     /locus_tag="VF516_30920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112201.1"
                     /translation="MEHRRDPTEQRVAEKHRRIAARIRREPGVIAESKIRLLRLMARE
                     PPPPDPVLQEWLDLFLMATPDQIAGFIESGTPRARRLRTSSPLLWIDR"
     gene            1221..1745
                     /locus_tag="VF516_30925"
     CDS             1221..1745
                     /locus_tag="VF516_30925"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HEX8112202.1"
                     /translation="MNRSVLDEVLQALSPIAGGRDLVMIGSQVVHAVTSDPPIEVVMS
                     RECDLLLDESDPLTNVIDEQFGLDSPRAAESSVYVDTVSSTFPFLPDGWEQRLVPFGP
                     AAPHLRCLEIHDLVLSKLAAGRLKDYELIAVLLDRKLADLATTRARIAAVPDLHMRAV
                     LAARLQIVLEGAPR"
     gene            2163..2960
                     /locus_tag="VF516_30930"
     CDS             2163..2960
                     /locus_tag="VF516_30930"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8112203.1"
                     /translation="MAHLEAISPHYHRGIGPFLFEPFARHTAERIRARAPNTVLETAC
                     CTGIVTRRLREALSGNAHLMASDPDERMLAVARRTVGAAGNVGWARADLCKLQFGDGD
                     FDAVVCQFGLMFAADKLAAVREARRVLRPDGSFLITTWAPLDRNPLVALVHRTLGAMF
                     REDPPPHLAQAPFDYGDPDMLNNLLLAGGFHDVVVDVVEKATSSPSAHELAVGLIEGY
                     PQIDEIKLHGKSRLGAAVTAVAQAIRRQFGDAPVRARITALVASAAA"
     gene            complement(2993..3655)
                     /locus_tag="VF516_30935"
     CDS             complement(2993..3655)
                     /locus_tag="VF516_30935"
                     /inference="COORDINATES: protein motif:HMM:NF012900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEX8112204.1"
                     /translation="MTRDTPAPAIHIKIKICGVTRPDDAAMVAAAGVDFVGLNFWPSS
                     KRYLPVERAPAVAEAARGSGRVGVVGVFVNARLDDIVAIARPLALDAIQLHGDETPQD
                     AAAVAHQTGCAVWKAVAVGRRSDLDRLDTWSVDAILLDTPTTGRGGSGRVFDWKLARD
                     ARRAYPTRRFVLAGGLSPDNVRAAIDAVSPWAVDVASGIESAPGVKDRAKLAAFLAAV
                     RG"
     gene            complement(3652..>4015)
                     /locus_tag="VF516_30940"
     CDS             complement(3652..>4015)
                     /locus_tag="VF516_30940"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="indole-3-glycerol-phosphate synthase TrpC"
                     /protein_id="HEX8112205.1"
                     /translation="AAPQLAELLAAARDHQLDALVEVHDLAEVDVALSAGTRLLGVNH
                     RDLRTFAVDMTLTAQIAPRVPADVVLVGESGIRTGDDIDALGRAGAHAVLVGEHLMRA
                     ASPGDALLALRGGSAGPR"
BASE COUNT          566 a         1482 c         1401 g          566 t
ORIGIN      
        1 ccggtcgcga tgccctcgcc ggccgcctcg accccgacca gccggacccc gggatcgccg
       61 gcgaacggcg cgaacagccc cgcggcgttc gagccgccgc cgacgcacgc gacgcaggca
      121 tccggcagcc ggccggtcgc cgacaggatc tgcgcgcgcg cctcgcggcc gatcaccgac
      181 tgcagatcgc gcaccatcca cggatacggg tgcggcccgg cgaccgagcc gatcaggtag
      241 aacgtgtcgc cgacgttggt cacccagtcg cgcagcgcct cgttcatcgc gtccttgagc
      301 gtgcgcgtcc cgctctgcac cgggtggacc tgggcgccga gcagccgcat ccgctcgacg
      361 ttcagcgcct ggcgcaccac gtcctcggcc cccatgtaga tctcgcacgg caggccgaac
      421 agcgccgcca ccgtcgcggt cgccacgccg tgctggccgg cgccggtctc ggcgatcagc
      481 cgccgcttgc ccatcttgcg ggcgagcacc gcctggccga tgcagttgtt gatcttgtgc
      541 gcgccggtgt ggttcaggtc ctcgcgcttc aggtagacct gcgcgccgac ctcctcggac
      601 agccggcgcg cgtggtagag cagcgacggc cggccgacgt agttgaccag caggtcctcg
      661 acctcggccc agtacgacgg atcgccgcgc gccgcccgcc acgccgcctc cagctcggcg
      721 atcgccggca tcagcgtctc ggcgacgtac tggccgccga acccgccgaa ccggccgccg
      781 tccggcgccg gcagcgcggc gatctcgtgg accgtcccgg tggtcgagac ctccgcctcc
      841 tcgatctcga cgtacgtggt cgtcttcatc cgcgtctcgc gtaccatagc ctgacgcgcc
      901 gcgaccagta cagcggccgc ccggttcgcg aagtatactg gtgcggtgga gcaccgccgc
      961 gacccgacgg agcagcgcgt ggccgagaag caccgccgga tcgccgcgcg gatccggcgc
     1021 gagccgggtg tcatcgcgga gagcaagatc aggctcctgc gcctcatggc gcgtgagccg
     1081 ccgccgcccg atccggtgct ccaggagtgg ctcgacctgt tcctgatggc cacgccggat
     1141 cagatcgccg gcttcatcga aagcggaacg cctcgcgcgc ggcgcctccg gacctccagc
     1201 ccgctgctct ggatcgaccg atgaaccgaa gcgtgctcga cgaggtgcta caggcgctgt
     1261 ctccaatcgc cggtgggcgc gatctcgtga tgatcggctc gcaagtggtt cacgcggtca
     1321 cgagcgaccc tcccatcgaa gtcgtgatgt ctcgcgagtg cgatctgctg ctcgacgaga
     1381 gcgatccgct cacaaacgtg atcgacgagc agttcgggct cgactctccg cgcgccgccg
     1441 aatcgtcggt ctatgtcgat accgtcagct cgacgttccc gttcctcccg gacggctggg
     1501 agcagcggct ggtgccattc ggccctgccg ctccacacct gcgctgcctg gagatccacg
     1561 atctcgtgct gtccaagctc gccgccgggc gactgaagga ctacgagctc attgccgtcc
     1621 tgctcgaccg caagctcgcc gatctcgcga ccacacgcgc ccggatcgct gcggtgcccg
     1681 atctccacat gcgcgccgtg ctggcagcgc gactgcagat cgtgctcgag ggcgcccctc
     1741 gctgatcgca gccagcggcc cgcagcaagc ggccgcaccc ggcggcccgg gccgccacgc
     1801 gactcgcggc gaagcggcgt agggccccgc actcccggcg ttttgtcagc ccgagcgccc
     1861 acgctcggtc gaggctgcag cgtgccgctg caagccgcga tgacatgcgg cccgacggtg
     1921 cgcatcgcga gcatcgctcc ggacactcga tcacgtgttc cgtatcgcgc gcagtgaacc
     1981 actgtgacgg cggcgcgcgt agcaatcatg cgcattcgca atagcaactt cgattgctga
     2041 atttgaggat cctgacgtgg caaacgtgtt acgccgggaa gtggagagca atcatgatgc
     2101 acgcgtgtga gtggacgcat cgccaaatca ccccggcgct cccgaggccg ctcctcgacg
     2161 acgtggcgca cctcgaagcg atctcgccgc actaccaccg cggcatcggc ccgttcctgt
     2221 tcgagccgtt cgctcgccac accgccgaac ggatccgggc gcgcgcgccg aacaccgtgc
     2281 tggagaccgc gtgctgcacc gggatcgtca cgcggcggct gcgcgaggcg ctgtccggca
     2341 atgcacatct gatggcatcc gatccggacg agcggatgct cgcggtggcg cgccgcacgg
     2401 tcggcgcggc gggcaacgtc gggtgggccc gcgcggacct gtgcaagctg cagttcggcg
     2461 acggcgactt cgacgcggtg gtctgccagt tcgggctgat gttcgccgcc gacaagctcg
     2521 ccgccgtgcg cgaggcgcgc cgcgtgctca ggcccgacgg cagcttcctg atcacgacct
     2581 gggcgccgct cgatcgcaac ccgctcgtcg cgctcgtcca tcgcacgctc ggcgcgatgt
     2641 tccgcgagga tccaccaccg cacctcgcgc aagcgccgtt cgactacggc gatcccgaca
     2701 tgctgaacaa cctgctgctc gcgggcgggt tccacgacgt ggtggtcgac gtggtcgaga
     2761 aggcgacgag ctcgccgagc gcgcacgagc tcgcggtcgg cctgatcgag ggctacccgc
     2821 agatcgacga gatcaagctg cacggcaaga gcaggctggg cgcggcggtc accgcggtcg
     2881 cccaggcgat ccgccggcag ttcggcgacg cgccggtccg ggcgcggatc accgcgctgg
     2941 tcgcgtcggc ggcggcgtga acatcgacgt gcacgcgacc gcggcgaccg gctcagccgc
     3001 ggaccgcggc gaggaacgcg gcgagcttgg cgcgatcctt gacgccgggc gccgattcga
     3061 tgccgctcgc gacgtcgacc gcccacggcg agaccgcgtc gatcgcggcg cggacgttgt
     3121 cgggcgacag cccgccggcg agcacgaacc gacgggtcgg gtacgcgcgg cgggcgtcgc
     3181 gcgcgagctt ccagtcgaac acccggccgc tgccgccgcg gccggtggtc ggggtgtcga
     3241 gcaggatcgc gtcgaccgac caggtgtcga gccggtcgag atcgctgcgc cggccgaccg
     3301 cgaccgcttt ccacaccgcg cagccggtct ggtgcgcgac cgcggcggcg tcctgcgggg
     3361 tctcgtcccc gtgcagctgg atcgcgtcga gcgccagcgg gcgggcgatc gcgacgatgt
     3421 cgtcgagccg ggcgttgacg aacacgccga ccacgccgac ccgaccgctg ccgcgcgccg
     3481 cctccgcgac cgccggggcg cgctcgaccg gcaggtagcg cttcgagctc ggccagaagt
     3541 tgaggccgac gaagtcgacg ccggccgccg cgaccatcgc ggcatcgtcg gggcgggtca
     3601 cgccgcagat cttgatcttg atgtggatgg ccggtgccgg cgtgtcacgg gtcatcgcgg
     3661 tcctgccgat ccgccccgca gggcgagcag cgcgtcgccg ggcgatgcgg cgcgcatcag
     3721 gtgttctccg accagcacgg cgtgggcgcc ggcgcggccc agcgcgtcga tgtcgtcgcc
     3781 cgtgcggatc ccgctctcgc cgaccagcac gacgtcggcc ggcacgcgcg gcgcgatctg
     3841 cgcggtcagc gtcatgtcga ccgcgaacgt gcggagatcg cggtggttga cgccgagcag
     3901 ccgcgtcccg gcggacagcg cgacgtcgac ctcggcgagg tcgtggacct cgaccagcgc
     3961 gtcgagctgg tgatcgcgcg cggcggcgag cagctcggcg agctgcggcg cggcg
//
LOCUS       DASVOR010001652         2295 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_192564,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001652 DASVOR010000000
VERSION     DASVOR010001652.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192564"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VF516_30945"
     CDS             complement(<1..208)
                     /locus_tag="VF516_30945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112206.1"
                     /translation="MRDLHPTGGARYLLERDGAADGPTARYRAAIYTPDAEFTAGALL
                     GDDGSAELGPTGAPDELHARLAALA"
     gene            complement(210..>2295)
                     /locus_tag="VF516_30950"
     CDS             complement(210..>2295)
                     /locus_tag="VF516_30950"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HEX8112207.1"
                     /translation="EHAPVKPPAKAPPRGPEHAVYSLVDNRLSAHLSRGGGLVVAAGS
                     AGFAKYLRFANLMKGGKKPWELRQTEGEVKVARLTGKSGTVYVPLTAAQAAAGKVRLR
                     AFARTDMTVSLKVNDGKDINGHLSAGWSTAELTVPGGQLKDGENALQLYMKSSGVELA
                     WLQVGGTAVGDDGGTAFYDPGQRALTLPDGGQMSWYVAVPDKARVTGDLADGSCTVRV
                     VATAEDGATVDGALTGTGSAVDLSALAGKAVRLDLTGAGCPVTALSHAVLAVPGEAPV
                     VKRGDPPRYVLFVVMDSLRADRVRTFNPRARPETPQFDKLAESSTLFMANYVQGNESQ
                     VSHASMWTSNYLAKHKAIEMSDHLPDKWVTIDEVAKQAGKFVAGASGNGYIRPARGFG
                     SGWDKFSNHIEKGLGLKGGEIIEHGLSFIAPKKDQPWFLYLGLIDTHVTWRAKSPWIE
                     RYDGGYKGRFGAEFGDDGPTGFAKDLNEHEKDHVRALYDSNVSYQDDQIGQLITKLQG
                     WGVWDKTMLIITADHGDEQWEDGRVGHGGSVRETLIHVPLIVHYPPVFPAVRITEGTE
                     GIDIVPTVADVLGAQADPEWQGMSLVGLANGAGGYPLMSVSSQYENAHGGRIGHWKLK
                     LIGTQTPKLYNLARDPDERTDLWGKPSVEIGARLVLDPMWLLRTWNVEWKKSQWGNAA
                     AVTSRFAADLGE"
BASE COUNT          292 a          821 c          823 g          359 t
ORIGIN      
        1 gggccagcgc cgcgagccgc gcgtgcagct cgtcgggcgc cccggtcggg ccgagctcgg
       61 cgctgccgtc gtcgccgagc agcgcgccgg cggtgaactc ggcgtccggg gtgtagatcg
      121 ccgcccggta gcgcgcggtg gggccgtcgg ccgcgccgtc gcgctccagc aggtagcgcg
      181 cgccgcctgt ggggtgcagg tcgcgcacgc tactcgccga ggtcggccgc gaaccgcgag
      241 gtcaccgccg ccgcgttgcc ccactgcgac ttcttccact cgacgttcca cgtccggagc
      301 agccacatcg gatcgagcac caggcgcgcg ccgatctcga ccgacggctt gccccacagg
      361 tcggtgcgct cgtcgggatc gcgcgcgagg ttgtagagct tgggggtctg ggtcccgatc
      421 agcttgagct tccagtggcc gatccgcccg ccgtgcgcgt tctcgtactg cgacgacacc
      481 gacatcagcg gatagccgcc ggcgccgttg gccagcccga ccagcgacat gccctgccac
      541 tcggggtcgg cctgggcgcc gagcacgtcg gcgacggtcg gcacgatgtc gatgccctcg
      601 gtgccctcgg tgatccggac cgcggggaac accggcgggt agtgcacgat cagcggcacg
      661 tggatcaggg tctcgcgcac cgagccgccg tggccgaccc ggccgtcctc ccactgctcg
      721 tcgccgtgat cggcggtgat gatcagcatc gtcttgtccc acacgcccca gccctggagc
      781 ttggtgatca gctggccgat ctggtcgtcc tggtagctga cgttggagtc gtacagcgcg
      841 cggacgtggt ccttctcgtg ctcgttgaga tccttggcga acccggtcgg gccgtcgtcg
      901 ccgaactcgg ccccgaaccg gcccttgtag ccgccgtcgt agcgctcgat ccacggcgac
      961 ttggcgcgcc aggtcacgtg ggtgtcgatc aggccgaggt agaggaacca cggctggtcc
     1021 ttcttcgggg cgatgaacga cagcccgtgc tcgatgatct cgccgccctt gaggccgagg
     1081 cccttctcga tgtggttgga gaacttgtcc cagcccgagc cgaagccgcg cgccggccgg
     1141 atgtagccgt tgcccgacgc accggcgacg aacttgccgg cctgcttggc gacctcgtcg
     1201 atcgtgaccc acttgtcggg caggtggtcg gacatctcga tcgccttgtg cttggcgagg
     1261 tagttcgagg tccacatcga cgcgtgcgag acctgcgact cgttgccctg gacgtagttg
     1321 gccatgaaca gggtcgacga ctcggcgagc ttgtcgaact gcggggtctc ggggcgggcc
     1381 ctggggttga acgtccggac ccggtcggcg cgcagcgagt ccatgaccac gaagaggacg
     1441 tagcgaggtg gatcgccccg cttgaccacc ggcgcctcgc ccggcaccgc gagcacggcg
     1501 tgggacagcg cggtcaccgg gcagcccgcg ccggtgaggt cgagccgcac cgccttgccg
     1561 gcgagcgccg agagatcgac cgccgagccg gtgccggtca gcgcgccgtc gaccgtcgcg
     1621 ccgtcctccg cggtggcgac cacgcggacc gtgcagctgc cgtcggcgag gtcgccggtg
     1681 acccgggcct tgtcgggcac cgccacgtac cagctcatct ggccgccgtc gggcagggtc
     1741 agcgcgcgct ggcccgggtc gtagaacgcg gtgccgccgt cgtcgccgac cgccgtcccg
     1801 ccgacctgga gccacgcgag ctcgacgccg gacgacttca tgtacagctg cagcgcgttc
     1861 tcgccgtcct tgagctggcc gcccgggacg gtcagctcgg cggtcgacca gccggcggag
     1921 aggtggccgt tgatgtcctt gccgtcgttg accttcaggc tgaccgtcat gtccgtcctg
     1981 gcgaacgcgc ggagccggac cttgccggcg gcggcctgcg ccgcggtcag cgggacgtac
     2041 accgtgccgg acttgccggt cagccgcgcg accttgacct cgccctcggt ctggcgcagc
     2101 tcccacggct tcttgccgcc cttcatcagg ttggcgaacc ggaggtactt ggcgaacccg
     2161 gccgagcccg cggcgacgac caggccaccg ccgcgcgaca ggtgcgccga cagccggttg
     2221 tcgaccagcg agtacacggc atgctcgggg ccgcgtggcg gcgccttggc cggcggcttg
     2281 accggcgcgt gctcg
//
LOCUS       DASVOR010001653         2364 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_105542,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001653 DASVOR010000000
VERSION     DASVOR010001653.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105542"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VF516_30955"
     CDS             <1..352
                     /locus_tag="VF516_30955"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8112208.1"
                     /translation="EAWIAADAGTIVNADRVRAQLEGAFVFGMTTALHAAITVKRGAV
                     EQSNFRDYPLTRIGEAPRKIHVELIAGDGPPGGVGEPGVPPVAPAILNAVFALTGQRV
                     RTLPLVRAPVVSPA"
     gene            complement(359..622)
                     /locus_tag="VF516_30960"
     CDS             complement(359..622)
                     /locus_tag="VF516_30960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112209.1"
                     /translation="MPDARWSRLKDCLLDRGWTSRDDVLYAPHAAMWFSRTSDDANLS
                     ALRDQIRSAAEASAEYVALHAEHTELHDALVSLADALDEVFQN"
     gene            complement(660..950)
                     /locus_tag="VF516_30965"
     CDS             complement(660..950)
                     /locus_tag="VF516_30965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112210.1"
                     /translation="MKPSDKPELVRAPPPGELQGEPPVVARLVVEIRSDGSRTIARGA
                     LEDAAAGQRVAIEAHGDSPLQLAIALARSLTQLPRLGARSAIRGLLGKRRDR"
     gene            1036..2127
                     /locus_tag="VF516_30970"
     CDS             1036..2127
                     /locus_tag="VF516_30970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112211.1"
                     /translation="MIYDYKKGSSQGPQGRAGAAGPSPGKQTLTEQLPAVQRKAVAGP
                     NPEGDTAADVAFGGIVKSVTADDVPLVSDASKPKDTEVAKLKKTDQVRPVDLGVGSAF
                     NKGSEGTGKTWWKVSVIAGPSTGKEGWVQVGSLGSILTVKNAGKLESTTKVGDGSVEV
                     HTGQELEREGAPDGDNNFALTYKGKDADDARWLQFIWREVVGVDDKGASTPVKETITT
                     TGGTYDLTDNGTYDKFGTPAKNNYNTDCPDKGNPFYETVGESDRTADSTKMIDLPGAA
                     VSQVKGAFKNGAKKVASRAHFSTFLIKGKKVAFQTAIDIAWDFQKKEDADAPPKGAHT
                     VSGSSEAASLPATIAERFHDQYPGFKDIK"
     gene            2138..>2364
                     /locus_tag="VF516_30975"
     CDS             2138..>2364
                     /locus_tag="VF516_30975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112212.1"
                     /translation="MSTSVRSAALIALIASAHGCGRPPSSPPARSSSMDITGTLELDG
                     RPLDDVALALVADDGRTVAVARSDAAGRFSLP"
BASE COUNT          391 a          826 c          846 g          301 t
ORIGIN      
        1 ggaggcgtgg atcgccgccg acgccggcac gatcgtcaac gccgaccgcg tgcgcgcgca
       61 gctcgagggc gcgttcgtgt tcggcatgac caccgcgctg cacgccgcga tcaccgtcaa
      121 gcgcggcgcg gtcgagcaat ccaacttccg cgactacccg ctgacccgga tcggcgaggc
      181 gccgcgcaag atccacgtcg agctgatcgc cggcgacggc ccgcccggcg gcgtcggcga
      241 gcccggtgtc ccgccggtcg cgccggcgat cctcaacgcg gtgttcgcct tgaccggcca
      301 gcgcgtccgc accctgccgc tggtccgcgc gccggtggtg tcgccggcgt gaaccggctc
      361 agttctggaa cacctcgtcg agcgcgtcgg ccaggctgac cagagcgtcg tgcagctccg
      421 tgtgctcggc gtggagcgcg acgtattccg ccgacgcctc ggcggcgctg cggatctggt
      481 cgcgcaacgc gctcaggttc gcgtcgtccg aggtccggga gaaccacatc gccgcatgcg
      541 gcgcatacag tacgtcgtcg cgcgacgtcc agccgcggtc gaggaggcag tccttgaggc
      601 gcgaccagcg ggcatccggc atgcccggtg atcttgcagg tcgcatgccg gccgcgcgct
      661 cagcgatccc ggcgcttgcc cagcaaaccc cggatcgccg atcgcgcgcc caggcgcggc
      721 agctgggtca gtgagcgcgc gagcgcgatc gcgagctgga gcggcgagtc gccgtgggcc
      781 tcgatggcca cgcgctgtcc ggcggcggcg tcctcgaggg cgccgcgcgc gatcgtgcgt
      841 gagccgtcgc tgcggatctc gaccaccagc cgcgcgacca ccggcggctc gccctgcagc
      901 tccccgggcg ggggggcgcg cacgagctcg ggcttgtcgg acggcttcat ggctgcagcg
      961 tagcgcaccc ggcgcggcgc gttaggaagt tcgcggtgcg gcggcgcgta tcatcgacta
     1021 cggaggagga gccgggtgat ctacgactac aagaagggtt cgagccaggg tccgcagggt
     1081 agagcgggcg ccgctggacc gtccccgggc aagcagacgc tgaccgagca gctccccgcg
     1141 gtccagcgca aggccgtggc cggcccgaat cccgagggcg acaccgcggc cgacgtggcg
     1201 ttcggcggca tcgtcaagag cgtcaccgcc gacgacgtcc cgctggtcag cgacgccagc
     1261 aagcccaagg ataccgaggt cgccaagctc aagaagaccg accaggttcg cccggtcgac
     1321 ctcggcgtcg gcagcgcgtt caacaagggc agcgagggca ccggcaagac ctggtggaag
     1381 gtctcggtga tcgcgggccc cagcaccggg aaggagggct gggtccaggt cggcagcctc
     1441 ggcagcatcc tgaccgtcaa gaacgccggc aagctcgaga gcaccaccaa ggtcggcgat
     1501 ggctcggtcg aggtccacac cggccaggag ctggagcgcg agggcgcgcc ggacggcgac
     1561 aacaacttcg cgctgaccta caagggcaag gatgccgacg acgccaggtg gctgcagttc
     1621 atctggcgcg aggtggtcgg cgtcgacgac aagggcgcct cgacgccggt caaggagacg
     1681 atcaccacga ccggcggaac ctacgacctg accgacaacg gcacctacga caagttcggc
     1741 accccggcga agaacaatta caacaccgac tgtccagaca agggcaaccc gttctacgag
     1801 acggtcggag aatcggaccg cacggcggac tcgaccaaga tgatcgacct gccgggcgcg
     1861 gcggtcagcc aggtcaaggg cgcgttcaag aacggggcga agaaggtcgc gtcgcgcgcg
     1921 cacttctcga cgttcctgat caagggcaag aaggtcgcgt tccagaccgc gatcgacatc
     1981 gcctgggact tccagaagaa ggaggacgcc gacgcgccgc ccaagggcgc gcacaccgtg
     2041 tcgggctcgt cggaggccgc gtcgctgcct gcgaccatcg ccgaacggtt ccacgaccag
     2101 tacccggggt tcaaggacat caagtagcct cgacatcatg tcgacctcgg tccggtccgc
     2161 cgcgctgatc gcgctgatcg cgtcggcgca cggctgcggg cggccgccgt cgtcgccccc
     2221 tgcgaggagc agctccatgg acatcaccgg cacgctcgag ctcgatggcc gtcccctgga
     2281 tgacgtggcg ctcgcgctgg tcgctgacga cggccggacg gtcgcggtcg cgcgcagcga
     2341 cgccgcgggc aggttctcgc tgcc
//
LOCUS       DASVOR010001654         3115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_309535,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001654 DASVOR010000000
VERSION     DASVOR010001654.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3115
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309535"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..702)
                     /locus_tag="VF516_30980"
     CDS             complement(16..702)
                     /locus_tag="VF516_30980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112213.1"
                     /translation="MRGLPAGFAVAVVVRVLGVLGLVGAAACAHGTGPDQTLDRYGLA
                     LKNHDFAAAYDLMSSGFRVKVSRDDYVRTMRDNAREVNETAERLRGKKGAMEVSAEFE
                     YGLGDTMRLIQEDGRWRIATYPLGFYDQSTPKAALRSFIRAYRLERWDVMLRFVPNSY
                     REKMTKEKMQAQFTGPSREQMENLINTLEANVDEPITERGNDARMSYGDRYTVQFLKE
                     DGAWKLKDLD"
     gene            complement(779..2026)
                     /locus_tag="VF516_30985"
     CDS             complement(779..2026)
                     /locus_tag="VF516_30985"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8112214.1"
                     /translation="MPGRARKLQILAALALTNLVAYAVRNELFVVLPDLKARFGLDDA
                     QFGLLQTVFMLPHAAATLPFGWAGDRYDRRYVIAVGMVLASVAGAAGAIGHSYAGLAA
                     SRACVGLGTAAVVPVANSILGEIFEGPLKASRMSLFNLGLFIGGVVGFAAGTALGFPA
                     VVVVLAVPGLALVVWILALPVPLHREPPPALRWWRYLAQLVPRFIADATALLAIRTLR
                     RVMLSATMMAFATGGYAAWLKEFLTRDKDMSDPEAVRLLVFTLFGGLGGILVGGRVAD
                     RLRRRWVSGRLWTIALGMALTIPYAVASIELPNSPALYAAGVATVFFMSWYHAPIAAT
                     VDDLAPPGKSVTAQGLVIFTMHMLGTAPASWVIGEISQRSTLHRAMWVPTVGLGIAAL
                     AMVIATTSFAADHARARGAIAMA"
     gene            complement(2057..>3115)
                     /locus_tag="VF516_30990"
     CDS             complement(2057..>3115)
                     /locus_tag="VF516_30990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112215.1"
                     /translation="VDRYRIDVTQAGALSVMVDGHDALDPVLEIEDASGTVIARSDRT
                     GARIREGVPNLGVTPGRYTAVVTAKKPPPAAAPKKKSAKRPAPEPARPAGGPYEITAS
                     MVAPAAGFEHEPDDDRGEANDLIAGDTGSGYLGWSGDADVWKISVEALSAKNALDVEL
                     SAVEGVALSLEISDGIGQPIVTRKGARGAPLVVRGFAPTVPQGAPPFYYLTVRGDRSN
                     PETAYQIRVTAKLIAPDREVEPNDTPETATSMADRKSLRAHWSTGDVDCFAVPPADAT
                     RTFDVSIDTPRDLDLAVDLLIEGKLMEKVDHPGKGAAEKLVMTVPPGAHAVIRVRGAD
                     LPGEDDYEITITESAAAP"
BASE COUNT          437 a         1152 c         1093 g          433 t
ORIGIN      
        1 ccctgcacac acgcttcagt cgagatcctt gagcttccag gcgccgtcct ccttgaggaa
       61 ctgcacggta tagcgatcac catagctcat gcgcgcgtcg ttgccgcgct cggtgatcgg
      121 ctcgtcgacg ttggcctcga gcgtgttgat caggttctcc atctgctcgc gcgacgggcc
      181 ggtgaactgc gcctgcatct tctccttggt catcttctcg cggtagctgt tgggcacgaa
      241 ccgcagcatc acgtcccagc gctcgagccg gtaggcccgg atgaacgagc gcagcgcggc
      301 cttgggcgtc gactggtcgt agaacccgag cgggtaggtc gcgatccgcc accggccgtc
      361 ctcctggatc agccgcatcg tgtcgcccag gccgtactcg aactcggccg agacctccat
      421 cgcgcccttc ttgccgcgca gccgctcggc ggtctcgttg acctcgcgcg cgttgtcgcg
      481 catcgtccgc acgtagtcgt cgcgcgagac cttgacgcgg aagcccgacg acatcaggtc
      541 gtacgccgcg gcgaagtcgt ggttcttgag cgcgagcccg tagcgatcga gggtctggtc
      601 ggggccggtg ccgtgcgcgc acgccgcggc gccgacgagc ccgaggacgc cgaggacgcg
      661 aacgacgacc gcgacggcga aaccggctgg gagaccccgc atgtggtggc gttatagcgg
      721 ccccccgcgc gatgcgccag ccggatcggc ccgggccacc ctcggcgagc ccggcggatc
      781 acgccatcgc gatcgcgccg cgcgcccgcg cgtggtcggc ggcgaagctc gtcgttgcga
      841 tcaccatcgc gagcgcggcg atgccgagcc cgacggtcgg cacccacatc gcgcggtgca
      901 gcgtcgagcg ctgcgagatc tccccgatca cccacgacgc cggcgcggtc cccagcatgt
      961 gcatcgtgaa gatcaccagg ccctgggccg tgacgctctt gcccggcggc gcgagatcgt
     1021 cgaccgtcgc cgcgatcggc gcgtggtacc agctcatgaa gaacaccgtc gccacgccgg
     1081 ccgcgtacag cgccgggctg ttcggcagct cgatcgacgc caccgcgtac gggatcgtca
     1141 gcgccatccc cagcgcgatc gtccacagcc ggccgctgac ccagcgccgg cgcagccgat
     1201 cggcgacccg gccgccgacc aggatgccgc cgagcccgcc gaacagcgtg aacacgagca
     1261 gccgcaccgc ctcggggtcc gacatgtcct tgtcgcgggt caggaactcc ttgagccacg
     1321 cggcgtagcc accggtcgcg aacgccatca tggtcgccga cagcatcacc cggcgcaggg
     1381 tgcggatcgc gagcagcgcc gtggcgtcgg cgatgaaccg cgggacgagc tgcgccaggt
     1441 agcgccacca gcgcagcgcc ggcggcggct cgcggtgcag cggcacgggc agcgccagga
     1501 tccacaccac gagcgcgagg cccggcacgg cgagcacgac gaccacggcc ggaaacccga
     1561 gcgcggtgcc ggcggcgaac ccgaccacgc cgccgatgaa caggccgagg ttgaacagcg
     1621 acatccggct ggccttgagc gggccctcga agatctcgcc gaggatcgag ttggcgaccg
     1681 ggaccaccgc ggcggtgccg agcccgacgc acgcgcgcga cgccgcgagc ccggcgtagc
     1741 tgtggccgat cgccccggcc gcgcccgcca cgctcgccag caccatgccg accgcgatca
     1801 cgtaacggcg gtcgtagcga tcgccggccc agccgaacgg cagcgtcgcg gcggcgtgcg
     1861 gcagcatgaa cacggtctgg agcagcccga actgcgcgtc gtcgagcccg aaccgggcct
     1921 tcaggtcggg caacaccacg aacagctcgt tgcgcaccgc gtacgccacg aggttggtca
     1981 gcgcgagcgc cgcgaggatc tgcagcttgc gggcgcgccc cggcatgcgg tcaccctacc
     2041 ctgccgcttg gcgatgtcac ggcgccgcgg cgctctcggt gatcgtgatc tcgtagtcgt
     2101 cctcgccggg cagatcggcg ccgcggaccc ggatcacggc gtgcgcgccg ggcggcaccg
     2161 tcatcaccag cttctccgcg gcgcccttgc cggggtggtc gaccttctcc atcagcttgc
     2221 cttcgatcag gaggtcgacc gcgaggtcga gatcgcgcgg ggtgtcgatc gagacgtcga
     2281 acgtccgcgt ggcgtcggcc ggcggcaccg cgaagcagtc gacgtcgccg gtgctccagt
     2341 gcgcgcgcag gctcttgcga tcggccatcg aggtcgcggt ctcgggcgtg tcgttgggct
     2401 cgacctcgcg atcgggcgcg atcagcttgg cggtgacccg gatctggtag gcggtctcgg
     2461 ggttggagcg atcgccgcgc accgtcaggt agtagaacgg cggggcgccc tgcggcaccg
     2521 tgggcgcgaa gccgcgcacc accagcggcg cgccccgcgc gcccttgcgg gtcacgatcg
     2581 gctggccgat cccgtcggag atctcgagcg acagcgcgac gccctcgacc gccgacagct
     2641 cgacgtcgag cgcgttcttg gccgacagcg cctcgaccga gatcttccag acgtcggcgt
     2701 cgccggacca gccgaggtag ccgctgccgg tgtcgccggc gatcaggtcg ttggcctcgc
     2761 cgcggtcgtc gtccggctcg tgctcgaacc cggccgccgg cgcgaccatg ctcgcggtga
     2821 tctcgtacgg cccaccggcg ggccgcgccg gctcgggcgc cgggcgcttg gccgacttct
     2881 tcttcggcgc cgccgccggc ggcggcttct tcgcggtcac caccgcggtg tagcgcccgg
     2941 gggtcacacc gaggttgggc acgccctcgc ggatccgcgc gccggtgcga tccgagcgcg
     3001 cgatcaccgt gccgctcgcg tcctcgatct ccagcaccgg gtcgagcgcg tcgtggccgt
     3061 cgaccatcac cgacagcgcg ccggcctggg tcacgtcgat ccggtagcgg tcgac
//
LOCUS       DASVOR010001655         1879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_309541,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001655 DASVOR010000000
VERSION     DASVOR010001655.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309541"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VF516_30995"
     CDS             complement(<1..617)
                     /locus_tag="VF516_30995"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8112216.1"
                     /translation="MEFRLDDEQEMIVQTIRRFADRDLRGWAADADRAGVPPERLQAM
                     GAELGLFLDAVPAANGGSLEGAYSHTLRALRGFELGRGCAGLAALLETNVEPALAVAA
                     WGTAAARQALFTSLAGGGLAAFAHDSRGSLEIEATPSALRVSGKLGPTPALAAASAVL
                     IAARDVLFLLPTAGLQRQPITPSGWRAARWATLVLEGERVPDDFVL"
     gene            complement(643..>1879)
                     /locus_tag="VF516_31000"
     CDS             complement(643..>1879)
                     /locus_tag="VF516_31000"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEX8112217.1"
                     /translation="LVAGADDADALWLDPAGLAHLTGDGKIQLLFDAAFVSQSVDYAR
                     IDSGGNQLASVSNQAPGMAIPTIAGALGIGDRLVIAGGIAAPYAGLHRYDDAGAQRYG
                     SVSLEGSAFVYVTVGAAYKLTDQLRVGATFMDVVSKLSSRVVLSGCPGQTVCAPEDPD
                     FDALAQVSQTDLVAPSGSVGVQYDAAPIATLGLAFQAPSRVSATGKFMSRLPSSAFFN
                     GAHIVGDQADLSFTLPAVLRAGIELHPTPELRVEAALDTELWSMHDEIAITPHDIRIE
                     DAPGVGTYKVGSIAIPQKFKTSFAPAIGAEWHGPQFMLGAGYSYETAAASPGYVSVLT
                     VDSAKHLVGIGGGYEAGGWQIGASAGLVLLADVQVPLADGKVTQLTPLRDQPSNVVVN
                     AGAYKSHYLIAGLRFARRL"
BASE COUNT          293 a          678 c          670 g          238 t
ORIGIN      
        1 agcacgaagt cgtccggcac gcgctcacct tcgagcacca gggtcgccca ccgcgcagcg
       61 cgccaccccg acggcgtgat cggctggcgc tggaggccgg cggtcggaag caggaacagg
      121 acatcgcgcg ccgcgatcag caccgcgctc gccgccgcca gcgccggggt gggccccagc
      181 ttgccggaca cccgcagcgc gctgggggtg gcctcgatct cgaggctgcc gcggctgtca
      241 tgcgcgaacg ccgccaggcc accgccggcg agcgacgtga acagcgcctg ccgggcggcg
      301 gccgtgcccc aggccgcgac cgccagggcg ggctcgacgt tggtctcgag cagcgccgcc
      361 agcccggcgc agccgcggcc gagctcgaag ccgcgcagcg cccgcagggt gtgtgagtac
      421 gcgccctcga gcgagccgcc gttcgcggcg gggaccgcat cgaggaacaa ccccagctcg
      481 gcgcccatgg cctgcaggcg ctccggtggg acaccggccc ggtcggcgtc cgccgcccag
      541 ccgcgcaggt cgcgatcggc gaaccggcgg atcgtctgga cgatcatctc ctgttcatcg
      601 tcgagccgaa actccatggg cacacctcgc ttccttggac gcctagagcc ggcgcgcaaa
      661 ccggagcccg gcaatcaggt agtggctctt gtacgcgccg gcattgacca ccacgttgct
      721 gggctgatcg cgcagcgggg tcagctgggt gaccttgcca tccgcaagcg ggacctggac
      781 atcggcgagc aggaccagac ctgccgacgc accgatctgc cagcctccgg cctcgtagcc
      841 gccgccgatc ccgaccaggt gcttggccga gtccacggtg agcaccgaga cgtagccagg
      901 agacgcagcg gcggtctcgt acgagtagcc ggcgcccagc atgaactggg gcccgtgcca
      961 ctcggcgccg atcgccgggg cgaacgacgt cttgaacttc tgcgggatcg cgatgcttcc
     1021 caccttgtac gtgccgacgc cgggggcatc ctcgatccgg atatcgtgcg gcgtgatggc
     1081 gatctcgtcg tgcatcgacc acagctcggt atcgagcgcc gcctcgaccc gcagctccgg
     1141 ggtcggatgc agctcgatcc cggcccggag gacggcaggc agcgtgaagg acaggtcggc
     1201 ctggtccccc acgatgtggg ccccgttgaa gaacgccgag ctcggcagcc gtgacatgaa
     1261 cttgccggtc gccgagaccc gcgagggcgc ctggaatgcc aggccgagcg tggcgatcgg
     1321 cgcggcgtcg tactgcacgc cgaccgatcc gctgggagcc acgagatcgg tctgggagac
     1381 ctgagccagc gcatcgaagt ccggatcctc gggcgcgcag accgtctgcc cggggcaccc
     1441 ggacaggacg acccgcgacg acagcttcga gaccacgtcc atgaaggtcg cgccgacgcg
     1501 cagctgatcg gtgagcttgt acgccgcgcc gaccgtgacg tacacgaacg ccgagccctc
     1561 caggctgacc gagccatagc gctgggcgcc ggcgtcgtcg tagcggtgca ggccggcgta
     1621 cggcgccgcg atgccgccgg cgatgaccag gcgatcgccg atcccgagcg ccccggcgat
     1681 cgtcgggatc gccatgccgg gcgcctggtt ggacaccgac gccagctgat tgccgccgct
     1741 atcgatccgg gcgtagtcga cggactggga cacgaacgcg gcgtcgaaca gcagctggat
     1801 cttgccgtcg ccggtgaggt gggccagacc cgcgggatcg agccacagcg cgtcggcgtc
     1861 gtcggcgccg gcgaccagc
//
LOCUS       DASVOR010001656         2253 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_75277,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001656 DASVOR010000000
VERSION     DASVOR010001656.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75277"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..745)
                     /locus_tag="VF516_31005"
     CDS             complement(140..745)
                     /locus_tag="VF516_31005"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8112218.1"
                     /translation="MRKIWPTKRSSGVQQTAGGEAPSAAGNAPPAVARHGRELLADHL
                     PVLRAVASRLCRGNAAETEDLVQDVLEKALRSIHALDLSQNPRGWMVTILHNLHIDRY
                     RRRARQGEHVPCDESLLSAPEALPAPDWAALTADDVRRAAAQLPAELRDAYVLFALDG
                     HTYAEVAAALGIPTATVGTRVLRARARLKEMLVAGLAAGCT"
     gene            complement(891..1643)
                     /locus_tag="VF516_31010"
     CDS             complement(891..1643)
                     /locus_tag="VF516_31010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112219.1"
                     /translation="MNRITLLPLVLSFAACSSSSPPTREQYDDTAQAIASTTATSGGG
                     GDVGSMTDSVTIAQGAMPTGFTLMGDGHFQGTHLGLAYSYSIVCKGIAGAVGPCGPTT
                     DQASVEVAWSGNLDTTYVDASVMRNGSWSITGLQSDTATFTGSGTFSFDATVRSIFRP
                     GAMATYSFDASAAYHAIRISTQQRQVIDGSASFDLSAHAQVSGTNHDSSASFDVHAQI
                     TFHADHTADLVLDGSQHYSLDLTTGGVVRVGT"
     gene            complement(1809..>2253)
                     /locus_tag="VF516_31015"
     CDS             complement(1809..>2253)
                     /locus_tag="VF516_31015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112220.1"
                     /translation="EVARLPQRIVWIEPCDGGVLLELADHALVQSSLVPGAPVTPLLP
                     ASSRSPYVSRDGKLIVGESANKRLAVVETATGSRWELPGYAVAQDLQAISPTARRFAQ
                     SGPRFLALWTLPLAPLELPRWLDERTNAATDGDDVLSWVWQAAPP"
BASE COUNT          329 a          744 c          871 g          309 t
ORIGIN      
        1 tcgagcgcgg cgagctgcag ggcgtcgtgg agcgccgcgc tgcacgacgg gcaggccgcg
       61 gcgtggagcg cgaacgcttc ggcctcggcg tcgccgagct caccgtcgac gtagcgcgag
      121 atctgagcgt gtggatgact catgtgcacc ccgctgcgag tcccgcgacg agcatctcct
      181 tcagccgtgc acgcgctcgc aggacgcgcg ttcccacggt ggccgtcggg atgccgagcg
      241 ccgccgcgac ctcggcgtac gtgtgaccgt cgagcgcaaa caggacgtag gcatcgcgca
      301 gctcggcggg gagctgcgcg gccgcgcgcc gcacgtcgtc agcggtcagc gccgcccagt
      361 ccggcgcggg cagcgcctcg ggcgcggaca gcaagctctc gtcgcacggg acgtgctcgc
      421 cctggcgcgc gcgccggcga tagcgatcga tgtgcaggtt gtggaggatc gtcaccatcc
      481 atccccgcgg gttctgcgac agatccagcg cgtggatcga gcgcagcgcc ttctcgagca
      541 cgtcctggac gagatcctcg gtctcggcgg cgttcccgcg gcacaggcgg ctcgcgaccg
      601 cgcgcagcac cggaagatga tcggcgagca gctcgcgacc gtgcctggca accgcggggg
      661 gcgcgttgcc ggctgccgag ggcgcctcgc ctccagcggt ttgctgcacg cccgagctac
      721 gcttggtcgg ccagatcttc cgcaccgcgg tgccttcgta tcaccccggc cactcgcgcg
      781 agaatcacag cgaaagatcc aggcccaaga tgtaggcaaa ggtcgcctat gtgctgcgtg
      841 atgcggcagc ccaggcgacc tcgccgcgat cgcggcggaa gcgcagtggg ctaggtgccc
      901 acgcggacca cgccgccggt ggtcaggtcg agcgagtagt gctggctgcc gtcgagcacg
      961 aggtcggcgg tgtggtcggc gtggaaggtg atctgggcgt ggacgtcgaa cgacgcgctg
     1021 gagtcgtggt tggtgccgga gacctgggcg tgggcggaca ggtcgaagct cgccgagccg
     1081 tcgatcacct ggcgctgctg ggtcgagatc cggatcgcgt ggtaggcggc gctggcgtcg
     1141 aacgagtagg tcgccatcgc gcccggacgg aagatcgagc gcacggtggc gtcgaacgag
     1201 aacgtgccgc tgccggtgaa cgtggcggtg tcggattgca ggccggtgat cgaccacgag
     1261 ccgttgcgca tcacgctggc gtcgacgtag gtggtgtcga ggttgccgga ccacgcgacc
     1321 tcgacgctgg cctggtcggt ggtggggccg cacgggccga ccgcgcccgc gatgcccttg
     1381 cagacgatcg agtagctgta ggcgaggccg aggtgggtgc cctggaagtg gccgtcgccc
     1441 atcagggtga agccggtcgg catggcgccc tgcgcgatgg tcacgctgtc ggtcatcgag
     1501 ccgacgtcgc cgccgccgcc gctggtcgcg gtggtcgagg cgatggcctg cgcggtgtcg
     1561 tcgtactgct cgcgggtcgg ggggctcgac gacgagcagg cggcgaacga caggacgagg
     1621 ggaagcaagg tgatgcggtt catgggatac ctcgcacccg gaagcagcaa gtcgcggacc
     1681 aggcgagccg ccgcgcgatc gcgagccgtg tcgcggagat acaggtggcg ggctcgcacc
     1741 tacctcgacg tacccacagt gcggatcgtc gggaaacacc acggggtggt ggattggcca
     1801 acgccgggct acggaggcgc ggcctgccac acccaggaca ggacgtcgtc gccgtcggtc
     1861 gcggcgttgg tccgctcgtc gagccagcgc ggcagctcga gcggcgccag cggcagggtc
     1921 cacagcgcga ggaagcgcgg gccggactgg gcgaaccggc gcgcggtcgg cgagatcgcc
     1981 tggaggtcct gcgcgacggc gtagcccggc agctcccagc gggagcccgt cgcggtctcg
     2041 accacggcga ggcgcttgtt ggccgactcg ccgacgatca gcttgccgtc gcggctgacg
     2101 tacggcgacc ggctcgacgc cggcaacagc ggcgtcaccg gcgcgccggg cacgagcgag
     2161 ctctgcacca gcgcgtggtc ggcgagctcc agcagcaccc cgccgtcgca cggctcgatc
     2221 cagacgatgc gctgggggag ccgggcgacc tcg
//
LOCUS       DASVOR010001657         6881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_396273,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001657 DASVOR010000000
VERSION     DASVOR010001657.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396273"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1150)
                     /locus_tag="VF516_31020"
     CDS             complement(<1..1150)
                     /locus_tag="VF516_31020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112221.1"
                     /translation="MARIAPKDLVGAIAAAKPGDRIELDSGELVGQVVIDKPLAIVGR
                     GSGTWLGNRSGPVLIIRRSGVELHDLQIEMTGDLQHPAILIDDGCELVQHNVTIRRGE
                     IRVDHPRLSFAAPAVIRLREPTPPVPAPTVPDPARRAQTQPPAPLAPAQTQPPPPPAR
                     AQTQPPPPPSTPSAQSVRTPAPVTPASSGAMGDRGVHRRYIVAIALMALCLLVFTVVR
                     AKLRGAAPSQDPRSAETGATVPPVPAVDEIELTRVLRTNLEVVSWSPDEEALALDLSY
                     GRDASSPARWRVRAVIDAPSEKVLHWQDLSGAAARVVSGEPRWPDHPAPSLPTEGFRP
                     GSVLAPSRLHVRWCEKPSATRFLTGVRSIGQLVELSWDSSGAQVTPEAC"
     gene            complement(1153..2508)
                     /locus_tag="VF516_31025"
     CDS             complement(1153..2508)
                     /locus_tag="VF516_31025"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112222.1"
                     /translation="MKLQDGTFATCEPRPLGQGGVGRVFRTTDGRSVVKLFHRPTPEL
                     EKALPLIVGDYRRIADGAEWCELYRWPDAIVVEPTLGVRMPLVADGFHELSWLILLKA
                     LASRPPSWRSWRSRLAIAAAIASAVTRLHGSGLAHSDLSPRNIWVDPLTHRVTLIDID
                     GLVVPGAVPPQVLGTPEYIAPELLAKQARPSTTTDLHSLAVLIYQLLLYRHPLRGPRV
                     CSPDPAKDDLIAFGPDGLFVDHPSDGSNRPVSEEWAGFWPTRILGPQLVEMFGRAFVA
                     GLRSPGARPPAGEWGHALTRLADRVVACSSKRCPEGFFPLSDGVAPICPWCRAPLKLR
                     KRTPVLHLYQGVGDHHYEPEPHWWIAGTEGRRIYPWHTAKGIAAATSMNGRAIAEVEH
                     DNGVWRLRNHELAELRVIRDGREQRVIPAGESVTLHDGLTLLLASPPRGRAIVVSRFD
                     L"
     gene            complement(2505..3356)
                     /locus_tag="VF516_31030"
     CDS             complement(2505..3356)
                     /locus_tag="VF516_31030"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX8112223.1"
                     /translation="MGLDELDTRPAPAAPAPDAIPPVATSDHGLVAGYRVVAASVVGR
                     GHVYQQRPCDDAFKIVVGCDGSRAWWFAAVADGAGSRALSRHGAEAATSAAAQRAAEA
                     LAAGWSLRDAIAAAVSIARTAQADRARQHGAGVADLSCTLLCLACGVDGPRMSLATFQ
                     VGDGLIAAIDDHGAIVPLAQADDEVPGATLFLESLDDHGWDARIRCEEPAAPPRGLVV
                     ATDGLADDLIPWSENGPILAGELDPMVALPDAGARLGEILSYEKHGSFDDRTLVVAWR
                     CSGPSCG"
     gene            complement(3367..4038)
                     /locus_tag="VF516_31035"
     CDS             complement(3367..4038)
                     /locus_tag="VF516_31035"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEX8112224.1"
                     /translation="MKLVGTSRRLPIYLVADTSASMHGAPIEAVNTGLKNFEAALRLD
                     PHALECAAISILTFASEAHQLTRLVEAGSFVPPALTAEGGTALGAALELLATALERDT
                     RARTTEYAGDWRPLVFLMTDGQPTDDWQPGLARYRAAAARWKSTLIAIGCGPGAAIDT
                     LSELTRTVMLMPDMTPDTIASLFDWISQSVRTASRAVSQHAAQGAQSGFADLPPPPQG
                     IRVVL"
     gene            4125..5108
                     /locus_tag="VF516_31040"
     CDS             4125..5108
                     /locus_tag="VF516_31040"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8112225.1"
                     /translation="MSATLEWERNGKRNLLTIVKQEVMIGYDPGCQVRGDAADLSARH
                     CRILHDGAQFVAEHISGVATLLNGVPITRHGLDSGDVIECGSFTVRFHEDRRTRPRPA
                     PAQPAVPPAAPPSPAALAPSPPPSPAPSPPPPAPDPAELQQLRAQLVRAEEDRDRDRE
                     EAIRLRAKLDQRAEDLRRADGELARTRTELDQASTNLAAAREELRVRDEQLVEKERLT
                     ATLHGQLDDRDRQLRTKDRELDSQASELQRLEGRARELFDANQGLRAELARAREQSRA
                     LETKHDDLARENARLQNNLSKVTNTLAEYQQSMQSLESQLRELQVLRDRHT"
     gene            5109..5507
                     /locus_tag="VF516_31045"
     CDS             5109..5507
                     /locus_tag="VF516_31045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112226.1"
                     /translation="MIESQQEEIQALGGARNRVQVLLEDLQRQHHHLDVSHELVKAEN
                     AKLSPENARLIAEAGRLQLEIQRMLRDRIPDRSLQDALIEIEALRKELGEAQRTLDRL
                     TVDRELFDRLRALAESHARDLPPGSRSPQS"
     gene            complement(5763..>6881)
                     /locus_tag="VF516_31050"
     CDS             complement(5763..>6881)
                     /locus_tag="VF516_31050"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8112227.1"
                     /translation="GADVIKVEKLPAGDDTRAYREPAIHGEPASFMILNRNKRSIAVD
                     LKTPGGLEVVRRLLAGADVLIENYRKGTLDRLGLGYDVVSRLNPRLVYCAVSGYGRTG
                     PYADKGGFDLIAQGFAGIMSITGEPGGPPAKSGTSVADINAGILAALGIVSALLARAT
                     TGRGQLVETSLMEAAVQQTYWQAALFFATGINAGPTGSAHILTAPYQAFATADGWINI
                     GGANQANWERIARVIGHPELTTDPRFATNGARMRHRDELVALIAGPLRTRPSADWIRA
                     FEAEGVPAGPVNRIGDMLADPQVAAREMVIEVDHPRAGRTRALGMPIKLSDARADMTR
                     PAPLLGQHSREVLDQLGYSAAEIEALQRAGAVLLGQGV"
BASE COUNT         1008 a         2449 c         2511 g          913 t
ORIGIN      
        1 gacatgcctc gggcgtgacc tgcgcgccgc tggagtccca gctgagctcg accagctgcc
       61 cgatcgaccg caccccggtg aggaaccggg tggccgatgg cttctcgcac catcgcacgt
      121 ggagccgcga gggtgcgagc acgctgccgg gccggaagcc ttccgtcggg agcgagggcg
      181 ctggatggtc cggccatctc ggctcccccg agacgacgcg cgctgctgcg cccgagaggt
      241 cctgccagtg gagcaccttc tcgctggggg catcgatcac ggcgcggacg cgccatcgcg
      301 ccgggctcga ggcatcgcgg ccatagctga ggtcgagggc caaggcctct tcatccgggc
      361 tccagctcac gacctcgagg ttcgtgcgca gaaccctggt cagctcgatc tcgtcgacgg
      421 ccggcaccgg aggaacggtc gccccggttt ccgctgagcg cgggtcctgg gacggggccg
      481 cgccccggag cttcgctctc accaccgtga acaccaggag gcacagcgcc atcagggcga
      541 tcgcgacgat gtacctgcga tggacaccgc ggtctcccat ggcaccgctc gaagccggcg
      601 tcaccggagc cggcgtgcgc accgactgtg cgctgggggt cgatggcggc ggcggcggtt
      661 gggtctgtgc gcgggccgga ggcggcggtg gctgggtctg cgcgggagcc agaggcgccg
      721 gcggctgcgt ctgcgcgcgg cgggcagggt cgggtaccgt gggagccggc acgggcggcg
      781 tgggctcgcg gagccggatg accgccggcg cagcgaagct cagccgcgga tggtcgacgc
      841 ggatctcgcc gcgccggatc gtcacgttgt gctgcacgag ctcgcagccg tcgtcgatca
      901 ggatcgcagg atgctggagg tctccggtca tctcgatctg gaggtcgtgc agctcgacgc
      961 cggagcgccg gatgatcagg accggaccgc tgcggtttcc gagccaggtg cccgagcccc
     1021 ggccgacgat cgcgagcggc ttgtcgatca ccacctggcc gaccagctcg ccgctgtcga
     1081 gctcgatccg gtcgccgggc tttgccgcgg cgatcgcgcc gaccaggtcc ttgggggcga
     1141 tccgcgccat cgtcagaggt cgaacctgct cacgacgatc gcgcggccgc gcggcggtga
     1201 cgcgagcagc agggtgagcc cgtcgtggag tgtcaccgac tcgccggccg gaatgacgcg
     1261 ctgctcgcgc ccgtcccgga tcacgcgcag ctcggcgagc tcgtggttcc gcagccgcca
     1321 gacgccgttg tcgtgctcga cctcggcgat cgcccgcccg ttcatcgagg tcgcagccgc
     1381 gattcccttc gcggtgtgcc agggatagat ccggcggccc tccgtgccgg cgatccacca
     1441 gtgcggctcg ggctcgtagt gatggtcacc gacgccctga tagagatgca gcaccggcgt
     1501 gcgcttgcgt agcttgagcg gagcacggca ccacgggcag atcggtgcga caccatccga
     1561 gagcggaaag aacccctcgg gacagcgctt gctcgagcac gccacgacgc gatcggcgag
     1621 ccgggtgagc gcgtggcccc attcaccggc gggcggccgg gcgccaggcg aacggaggcc
     1681 ggcgacgaac gcgcggccga acatctccac cagctgcggc cccaggatcc gcgtcggcca
     1741 gaatccggcc cactcctcgc tcaccggccg gttcgaacca tcgctcggat gatcgacgaa
     1801 caggccgtcg ggaccgaacg cgatgagatc gtccttggcc ggatccggcg agcagacccg
     1861 cgggccgcgc aacgggtggc ggtacagcaa gagctgatag atcagcacgg cgagcgagtg
     1921 cagatcggtc gtggtgctcg gccgcgcctg cttcgccagc agctccggcg cgatgtactc
     1981 cggcgtcccg agcacctgcg gaggcaccgc gccggggacg accagcccgt cgatgtcgat
     2041 cagcgtgacc cgatgggtga gcgggtcgac ccagatgttg cggggcgaca gatccgagtg
     2101 cgccaggccg ctgccgtgca gccgggtcac ggccgacgcg atcgcggcgg cgatcgccag
     2161 gcgcgaccgc cacgatcgcc aggacggcgg ccgcgacgcc agcgccttga gcaagatcaa
     2221 ccagctcagc tcgtggaagc cgtcggcgac cagcggcatg cgaacgccca gcgtcggctc
     2281 gacgacgatc gcatcgggcc agcgatacag ctcgcaccac tccgcgccgt cggcgatgcg
     2341 gcggtaatcg ccgacgatca gaggcagcgc cttctcgagc tccggggtcg gacggtgaaa
     2401 cagcttgacc acgctgcgcc cgtcggtcgt gcggaacaca cgccccacgc cgccctggcc
     2461 gagcggcctg ggctcgcagg tggcgaacgt gccgtcctgg agcttcaccc gcatgacggt
     2521 ccgctgcagc gccaggccac cacgagcgtg cggtcgtcga acgagccgtg cttctcgtac
     2581 gacaggatct cgccgagccg ggcgccggca tctggcagcg ccaccatcgg gtcgagctcg
     2641 ccggcgagga tcggcccgtt ctccgaccac gggatcaggt cgtcggcgag gccgtcggtg
     2701 gcgacgacca gtccgcgcgg tggcgccgcc ggctcctcgc agcggatccg cgcatcccag
     2761 ccgtgatcgt cgaggctctc gaggaacagc gtggcgccgg gcacctcatc gtcggcctgc
     2821 gcgagcggca cgatcgcgcc gtggtcgtcg atcgccgcga tcaagccgtc gccgacctgg
     2881 aacgtcgcca gcgacatgcg cgggccgtcg accccacacg cgaggcagag cagcgtgcac
     2941 gacaggtccg cgacgccggc gccgtgctgc cgtgcgcgat cggcctgcgc cgtgcgtgcg
     3001 atcgacaccg cggcagcgat cgcgtcgcgc aacgaccagc cggccgccag ggcctcggca
     3061 gcgcgctgcg ccgcggcaga ggtcgccgcc tcggcgccgt gccgcgacag cgcacgcgaa
     3121 ccggcgccat ccgccaccgc ggcgaaccac cacgcgcggc tgccatcgca gccgacgacg
     3181 atcttgaatg cgtcgtcaca cggccgctgc tggtacacgt ggccgcgccc gacgacgctc
     3241 gccgccacga cgcggtatcc cgcgacgagc ccatggtcgc tggtcgcgac cggcgggatc
     3301 gcgtcgggtg ccggcgccgc gggcgcaggt cgggtgtcga gctcgtcgag ccccatctcg
     3361 cacccgctag agcaccaccc ggatgccctg cggcggcggc ggcagatcgg cgaacccgga
     3421 ctgcgcgccc tgtgcagcgt gctggctgac cgcccggctc gcggttcgca ccgactggct
     3481 gatccagtcg aacagcgacg cgatcgtgtc gggtgtcatg tccggcatca gcatgacggt
     3541 cctggtgagc tcgctcagcg tgtcgatcgc ggcgcccggg ccgcagccga tcgcgatcag
     3601 cgtgctcttc cacctggcgg cggcggcgcg gtaacgcgcg agccccggct gccagtcgtc
     3661 ggtcggctgc ccgtcggtca tcaggaagac cagcggccgc cagtctcctg cgtattcggt
     3721 ggtgcgcgcg cgcgtgtcgc gctccagcgc ggtcgcgagc agctcgagcg cggcgccgag
     3781 cgccgtgccg ccctcggcgg tgagcgcggg tgggacgaag ctccccgcct cgaccaggcg
     3841 cgtcagctgg tgcgcctcgc tggcgaacgt gaggatgctg atcgcagcgc attcgagcgc
     3901 gtggggatcg agccggagcg ccgcctcgaa gttcttcaag ccggtgttga cggcctcgat
     3961 cggcgcgccg tgcatcgacg cggacgtatc ggcgacaagg tagatcggaa gccgccggct
     4021 ggttcctacg agtttcacca gatgctcccc agtggtccac cgagcatcct actgcggcgc
     4081 gatccggaag tccatcgacc actacagctg atactgtgac gttgatgtct gcgacactcg
     4141 aatgggagcg caacggaaaa cgcaacctgt tgacgatcgt caagcaggag gtgatgatcg
     4201 ggtacgaccc gggttgtcag gtgcgcggcg atgccgccga cctgtccgcc cggcactgcc
     4261 gtatcctgca cgacggcgcg cagttcgtcg cggagcacat ctccggcgtg gccacgctgc
     4321 tcaacggcgt cccgatcacg cgtcacgggc tcgacagcgg cgacgtgatc gagtgcggca
     4381 gcttcacggt gcggttccac gaggacaggc gcacccggcc caggccggca ccggcccagc
     4441 cggcggtgcc gcccgcggcg ccgccgtccc ccgcagcact tgcaccatcc ccaccgccgt
     4501 cccccgcgcc gtcaccaccg ccgccggcgc cggatccggc agagctccag cagctgcggg
     4561 cccagctggt gcgcgccgag gaggaccgcg accgtgaccg cgaggaggcg atccggctgc
     4621 gcgcgaagct cgaccagcgc gccgaggatc tgcgccgcgc cgacggcgag ctcgcccgga
     4681 cccggacgga gctcgaccag gcgagcacga acctggccgc ggcgcgcgag gagctccggg
     4741 ttcgcgacga gcagctcgtc gagaaggagc ggctgaccgc gacgctgcac gggcagctcg
     4801 acgaccgcga ccggcaactc cgcaccaagg accgggagct cgactcgcag gcgagcgagc
     4861 tgcagcggct cgaggggcgg gcccgcgagc tgttcgacgc caaccagggc ctccgcgccg
     4921 agctcgcgcg ggcccgcgag cagtccaggg cgctcgagac caagcacgat gacctcgcgc
     4981 gggagaacgc gcggctgcag aacaacctga gcaaggtcac caacaccctg gccgagtacc
     5041 agcagtcgat gcagtccctc gagagccagc tcagggagct ccaggtcctg cgcgaccggc
     5101 acacgtaggt gatcgagtca cagcaggagg agatccaggc gctgggaggt gcgcgcaacc
     5161 gggtccaggt cctgctcgag gacctccagc gacagcatca ccacctcgac gtgagccacg
     5221 agctcgtcaa ggccgagaac gcgaagctct cgcccgagaa cgcgaggctg atcgccgaag
     5281 ccggccggct ccagctcgag atccagcgca tgctgcgcga ccgcatcccc gaccgctcgc
     5341 tgcaggacgc gctgatcgag atcgaggcgc tccgcaagga gctcggcgag gcgcagcgca
     5401 ccctcgaccg gttgaccgtc gatcgcgagc tgttcgatcg acttcgcgcg ctcgccgaat
     5461 cgcacgcgcg cgacctccca ccgggatcgc gatcgcctca gtcgtgacgg tcgacggcgt
     5521 ggtgaatatc cgggccagga ggcgcactcg cgaggcgcca acgcgaggcc gccgtgggcg
     5581 accgcgagca ggatggggtc ggacgccgcg accgtgattt ggattggcag gtcttgtccc
     5641 gcacatacct cgcgccgtcc gcgcatgggc cggtggtgat tcggcgggcg cggctccgac
     5701 acccatccgc gcagcagccc acggcgagtc tcgcgtgtga gcgttctccc gggcgctacg
     5761 cgctacacgc cctggccgag cagcacggcg ccggcgcgct gcagggcctc gatctcggcg
     5821 gcggagtagc cgagctggtc gagcacctcg cggctgtgct ggccgagcag cggggcgggg
     5881 cgggtcatgt cggcgcgggc gtcggacagc ttgatcggca tgccgagcgc ccgggtgcgg
     5941 ccggcgcgcg ggtggtcgac ctcgatcacc atctcgcgcg cggcgacctg cgggtcggcg
     6001 agcatgtcgc cgatccggtt gaccgggccg gccggcacgc cctcggcctc gaacgcgcgg
     6061 atccagtccg ccgacggccg ggtgcgcagc ggcccggcga tcagcgcgac cagctcgtcg
     6121 cggtggcgca tgcgggcccc gttggtggca aaccgcgggt cggtggtgag ctcggggtgg
     6181 ccgatcacgc gggcgatccg ctcccagttg gcctggttgg cgccgccgat gttgatccag
     6241 ccgtcggcgg tggcgaacgc ctggtacggc gcggtgagga tgtgggccga gccggtcggc
     6301 ccggcgttga tgccggtcgc gaagaacagc gccgcctgcc agtaggtctg ctggaccgcg
     6361 gcctccatca gcgaggtctc gacgagctgg ccccggccgg tggtcgcgcg cgccagcagc
     6421 gcggagacga tgccgagcgc ggcgaggatg ccggcgttga tgtcggcgac cgacgtgccg
     6481 ctcttggcgg gcggcccgcc gggctcgccg gtgatgctca tgatcccggc gaagccctgc
     6541 gcgatcaggt cgaagccgcc cttgtcggcg tacgggccgg tgcggccgta gcccgacacc
     6601 gcgcagtaca ccagccgcgg gttgagccgg ctcaccacgt cgtagccgag gccgagccgg
     6661 tcgagcgtgc ccttgcggta gttctcgatc aggacgtcgg cgccggcgag caaccgccgg
     6721 acgacctcga gcccgccggg ggtcttgagg tcgaccgcga tgctgcgctt gttgcggttg
     6781 aggatcatga acgacgccgg ctcgccgtgg atcgccggct cgcggtaggc gcgggtgtcg
     6841 tcgccggcgg gcagcttctc gaccttgatc acgtcggcgc c
//
LOCUS       DASVOR010001658         3024 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_425494,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001658 DASVOR010000000
VERSION     DASVOR010001658.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425494"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..601
                     /locus_tag="VF516_31055"
     CDS             212..601
                     /locus_tag="VF516_31055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8112228.1"
                     /translation="MNREENMFIAYDVALDLVRALRPVVEQIRKYSPEAADQDERAAS
                     RIVLNLAEGGRRHGRDPRRFWDMAHGSAGEIRGALDLADAWGWQVESARARALLDREL
                     GLLWGLTRRGRVKDGSLPASGNRTRPR"
     gene            1498..2559
                     /locus_tag="VF516_31060"
     CDS             1498..2559
                     /locus_tag="VF516_31060"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX8112229.1"
                     /translation="MSGRSLLDRLLDEAGALRGIEATAADAADAALDELASMSGQASA
                     AQGGGEQRIHRDASALRDRAAAARKAFNRADALVELAQGYLRGDRPDRSPIEVMVTIP
                     VSVLRGELTDPVEVAELGESFLSREAARRLSCDAGVVEIIEDEQGAPLPVGRKRRTIA
                     GALRRALHRRDRSCTYPGCTNRLFLEGHHIKHWADGGETSLLNALLVTRKRLPMARRW
                     SGMQLVGMSTPMPRFACPIPMVRSSITFVKPPLRCITTHAGSRRLVPVFIVGARTCYL
                     GARGAGHSLADVAKRIGASSRNAYARYEHGTSMPKLDTVQEILKAVAPELALTLGPRK
                     GPKAAPRTAKAKRRSGRAA"
     gene            complement(2722..>3024)
                     /locus_tag="VF516_31065"
     CDS             complement(2722..>3024)
                     /locus_tag="VF516_31065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112230.1"
                     /translation="EPRALVGREPRALAPRKLFSDVQEPLGTLLRLGVLTFGAGGWVG
                     MAGIRLSLAIVVYTFAVLAFPARRKLIGVGHELDAMLAEGQGGFSDIAKSAMARKR"
BASE COUNT          456 a          967 c         1140 g          461 t
ORIGIN      
        1 tttgatcccg aggatttggc cgcgcggttg gaagctgggg acggctatct aaagcacgga
       61 gcctgggcga aggcgccacc gccgcgaaga cgtgcgttgg gaaagacccc gtgcaccgac
      121 gagggcgttc gtcacgggtc ctgcgcggat cggtcgcgac gggcgtagcc cagccgcgga
      181 ggtcgtcggc agagggcgtt tcccttcaga gatgaatcgc gaggaaaaca tgttcattgc
      241 ctatgacgtc gcgctggatc tggttcgcgc tctgcggccc gtggtcgagc agatccggaa
      301 gtacagcccc gaggcggccg atcaggacga gcgtgcggcc agtcgcatcg tgctcaacct
      361 cgccgagggc ggtcggcgcc atggccgcga tccacggcgg ttctgggaca tggcgcacgg
      421 cagtgccggg gagatccgcg gtgcgctcga cctggcagat gcgtggggct ggcaggtcga
      481 gagcgcgcgt gcgcgtgcgc tgctcgatcg cgagctgggc ctcttgtggg gactcacccg
      541 gcgcggccgc gtcaaggacg gatcgcttcc agcgtcagga aacaggacgc gaccacggtg
      601 acatggggcc ggacgcgcgg gccggcgcgg ggttgtgggc ccaagagccc gctggacgac
      661 tgcacatctg cgggcaggag cagtgtcgtg aggcgtctgc cggctccatc ccgaggaagc
      721 cgagctggtc tggaccatgc tcgaccacgc cgcgagccgg ctggcacgcg aggcggctgc
      781 cgctgcgagc gagactgtgg ggcaggattc cgcggaatct acggggggca gtgaggcgaa
      841 ttcgcgcgat gcggagttta cgggcagcgc ggaggaggaa tcgcgcgatg cggagcccgc
      901 tggcagcgct gacggacaat cggaccgcgc ggagtctgcc cagagcacgg atgcagggtc
      961 ctgcgatccg gagcctgccg gaatccatgc ccgtgctgcc gtcggctccg cacaagctgc
     1021 ggcatcgctg tcggtttcga tgtcgccgct cgcgatcgcc tggcaggtgc acgtcggtcc
     1081 ccgcaccgac caggactcgg ccggatggtc gcagatggcc tcgtcgcctt gcgccgcgaa
     1141 cgatatgccc acgtgcctgg agagccgttc agtcgacgat tccgcagaat cgccagccgc
     1201 agctgatccg tgttgtactg ctgaaagcgc ctccgccgcg gcctcgcctg ggaaactggc
     1261 cgagtgggcg ggtccctcga ccgccgcgag ctcgcctggg gggagaccga gtgggcggct
     1321 tcctctgtag tcggggtctt ggccggcggc ccgacagcgt cggcgggttt ctccatggcc
     1381 agggcctcgg ccggggaaca gctggagtgg gcgggctcct ccacggccgg ggccgcagcc
     1441 ggggatcggg tggagtgggc tggctcgcta ttccacggcc aggctgcgtg ctccgaggtg
     1501 tcggggcgct cgctgctcga tcgcctgctg gacgaagccg gtgcgctccg cggcatcgaa
     1561 gcgaccgctg ccgacgccgc cgatgccgcc ctggacgagc tcgcgtcgat gtcgggccag
     1621 gccagtgcag cacaaggggg gggggagcag cggatccatc gcgacgccag tgcgctccgc
     1681 gatcgcgcgg cggcggcgag gaaggccttc aaccgtgctg acgccctggt cgagctcgcc
     1741 cagggctacc tccgcggtga ccggccggat cgttcaccga tcgaggtcat ggtcacgatc
     1801 ccggtgagcg tgctgcgtgg cgagctgacc gatccggtcg aggtggcgga gctcggcgaa
     1861 tcgttcctgt cccgcgaggc cgcgcgccgg ctgagctgcg acgcgggtgt cgtcgagatc
     1921 atcgaggatg aacagggtgc gccgctgccg gtgggacgca agcgccgcac gatcgcgggc
     1981 gcgctccggc gagcactgca tcgccgcgat cgatcgtgca cgtaccccgg ttgtaccaac
     2041 cgcctgttcc tggagggcca tcacatcaag cactgggcag atggcggcga gacaagtctc
     2101 ttgaatgcct tattggtgac cagaaagcgc ctgccgatgg ctcgcaggtg gagcggaatg
     2161 cagctggtcg ggatgtccac gccgatgccg cggtttgcat gtccgattcc gatggttcgc
     2221 tcgagtatca ccttcgtgaa gccgccgttg aggtgcatca cgacgcatgc gggttcgcgc
     2281 cgtttggttc ctgtcttcat cgtcggagcg cgtacatgct acctcggggc gagaggggcc
     2341 ggtcattcct tggccgacgt ggccaagcgg atcggcgcca gctcgcgcaa cgcctacgcg
     2401 cgctacgaac acggaacctc gatgccgaag ctggacacgg tacaggagat cttgaaggcc
     2461 gtggcgcccg agctcgcgct caccttgggt cctcgcaagg gcccgaaagc agcaccgcgg
     2521 acggcgaagg ccaagcggcg ctctgggcgt gcggcctgac gctggcggca cgagcgattc
     2581 gatcggccgt cgggggagcg atcccgttgg ccgtccagcg tccgatccgg tcggctgcct
     2641 gcacccggat ccgttcgcag gcctcgtgac acaggcgaga ggacatgttc ggggatgtgc
     2701 gtcgcgtcgc gcgggggctg gtcagcgctt gcgggccatc gcgctcttgg cgatgtcgga
     2761 gaagccgccc tgaccctcgg cgagcatggc gtcgagctcg tggccgacgc cgatcagctt
     2821 gcggcgggcc gggaacgcga gcaccgcgaa ggtgtagacc acgatcgcga gcgacaggcg
     2881 gatgccggcc atgccgaccc agccgccggc gccgaacgtc agcacgccga gccggagcag
     2941 ggtgccgagc ggctcctgga cgtcggagaa cagcttgcgc ggcgcgagcg cgcggggctc
     3001 gcggccgacc agggcgcgcg gctc
//
LOCUS       DASVOR010001659         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_221706,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001659 DASVOR010000000
VERSION     DASVOR010001659.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221706"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..296
                     /locus_tag="VF516_31070"
     CDS             144..296
                     /locus_tag="VF516_31070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112231.1"
                     /translation="MPDDDLDPEQRAALHASLDRALEDSEAGRGVDAAEYLRRYRARH
                     KARRAR"
     gene            389..>1541
                     /locus_tag="VF516_31075"
     CDS             389..>1541
                     /locus_tag="VF516_31075"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8112232.1"
                     /translation="MASEGQTGPTGATYHVLLIGIDAYPGRPLEGCVNDIDAVQRVLL
                     DDRLAIPPACIRRLASPLPGTRHETAIAEQPATFANIHAALTELGSERVRPGDRVFLY
                     YSGHGKRVVVKGPDGPTFQREALVPVDFEAAPGFPPGSDAFLFDFELNGLLAAIAART
                     SSVAVMLDCCHAAGTPRDGGAVRSFDRGARDREPIADPARKLAATRGAGSGDRTGSVH
                     TCQVVSACLANEFSRENERDGVRNGVFTSAFVAAVRGATGDLRTLTWDRIWHAMLNEV
                     MLRHPGQSPRMDGSPRRAVFGGPPGDGDAGLSVSRDGDGYRIAAGTMADITLGTELAI
                     YGPDPARFPPLGSTADVAVRLGVVRVTEADPATARAAAIGPAFELPPGAR"
BASE COUNT          203 a          567 c          559 g          212 t
ORIGIN      
        1 ttccacgccg gcgccagcct gcagtaccag ctcgagaacc gccactacgc ggtcggcctg
       61 gccgccgacg gcttcctctt gccccagttc gcctcgatcc gcgccatcga ctcgcggctg
      121 ttccttcgct acacgtactg acgatgccag acgatgacct cgaccccgag cagcgcgcgg
      181 ccttgcacgc gtcgctggat cgcgcgctcg aggactccga ggccggccgc ggagtggacg
      241 ccgcggagta cctccggcgg tatcgagctc gccacaaagc tcgtcgtgct cggtagttag
      301 cgcccccttc gaatccagcg tttcgctgtc gtgaaacgct gggaccggtc gccaacgaga
      361 cagcgcgctg acgcccgcgg aggaagccat ggctagcgag ggacagaccg gaccgactgg
      421 agcgacgtac catgtcctgc tgatcgggat cgatgcctac cccggccgcc cgctcgaggg
      481 ctgcgtcaac gacatcgacg cggtgcagcg cgtgctgctc gacgaccggc tcgcgatccc
      541 gccggcctgc atccggcggc tggcgtcgcc gctgcccggg acgcggcacg agaccgcgat
      601 cgccgagcag ccggcgacgt tcgccaacat ccacgccgcg ctgaccgagc tcggctcgga
      661 gcgggtccgg cccggcgacc gggtgttcct ctactactcc gggcacggca agcgcgtggt
      721 cgtgaagggc ccggacgggc cgacgttcca gcgcgaggcg ctggtgccgg tcgacttcga
      781 ggcggcaccg ggatttccac cgggatccga cgcgttcctg ttcgacttcg agctcaacgg
      841 cctgctcgcc gcgatcgccg cgcgcacgtc gtcggtcgcg gtgatgctcg actgctgcca
      901 cgccgccggg acgccgcgcg acggcggtgc ggtccgctcg ttcgaccgcg gcgcgcgcga
      961 ccgggagccg atcgccgacc cggcgcgcaa gctcgccgcc acgcggggcg cggggtcggg
     1021 cgacaggacg ggcagcgtcc acacgtgcca ggtggtgtcg gcgtgcctcg ccaacgagtt
     1081 ctcgcgcgag aacgagcgcg acggcgtgcg caacggcgtg ttcacgtcag cgttcgtcgc
     1141 cgcggtgcgc ggcgccacgg gcgatctgcg cacgctgacg tgggaccgga tctggcacgc
     1201 gatgctgaac gaggtgatgc tgcggcatcc cgggcagagc ccgcgcatgg acggcagccc
     1261 gcggcgcgcg gtgttcggcg gtccaccggg cgacggcgac gccggtctgt ccgtgagccg
     1321 cgacggcgat ggctaccgga tcgcggccgg gacgatggcc gacatcaccc tcggtaccga
     1381 gctggcgatc tacggccccg atccggcgcg gttcccgccg ctcggctcga cggccgatgt
     1441 cgcggtccgg ctcggcgtcg tgcgggtgac cgaggcggat cccgcgaccg cgcgcgccgc
     1501 cgcgatcggc ccggcgttcg agctgccgcc cggggcgcgc g
//
LOCUS       DASVOR010001660         2701 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_279185,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001660 DASVOR010000000
VERSION     DASVOR010001660.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279185"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VF516_31080"
     CDS             complement(<1..298)
                     /locus_tag="VF516_31080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112233.1"
                     /translation="MTARKLRFPMFFKFLAGCLALAALLIVGGTFVVRNETRLRSRGN
                     YLQKQDRRLAGYLDRVGRDMTATLELLVSDAELRTAMSQLAGVIKPAPTAAPVPR"
     gene            complement(295..780)
                     /locus_tag="VF516_31085"
     CDS             complement(295..780)
                     /locus_tag="VF516_31085"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX8112234.1"
                     /translation="MVSPGAGGPLRLVCFELRGQELALPIADVRETLPVPPITRVFLT
                     PPCLAGVFSLRGDIVPVIDLGVLLGLPATAAGDDSKIVVVDCAAGVAGIVVDRLRDLR
                     TVEPPLEPPPAHLPGEVAGLLAGMVATPTGSVRVLDARAVLTAPPLAPLARADAEGRM
                     S"
     gene            complement(791..2059)
                     /locus_tag="VF516_31090"
     CDS             complement(791..2059)
                     /locus_tag="VF516_31090"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEX8112235.1"
                     /translation="MPTRHPTQPPGLDEEEDTSRADVSGEIRLPATLEGAGPLPDRAE
                     VVIVGGGVVGLAIAYNLARRGLDDVVVIERGYLAEGASGRNGGGVRQQWSTELNIRLM
                     QESVELCRRFAVDLGVNVWFRQGGYLFLARSAQEVARLEKNIAIQNRCGVPTRMLEPD
                     KARDIVPELDLTGIIAAAYNPTDGILFPWPFLWGYARQAAAHGVRIFTQTPVGGLEPQ
                     KGGGYLVHTPRGAVRARRLVNATGAWSPRLAEMIGVHVPTYPIRHEICSSEPLKPFLR
                     PMVSELTSGLYCSQSMRGEIVGGVTIPGHDSTYGMGSTLEFLATYARRLVRLMPILGE
                     IKVLRQWAGPYDQSPDGNPILGAAPGHPDFFLACGFVGHGFMMAPIVGKLYGEWLTGG
                     EPHEIFARYTLGRFSGGAPPVPEKEDFNIG"
     gene            complement(2059..2370)
                     /locus_tag="VF516_31095"
     CDS             complement(2059..2370)
                     /locus_tag="VF516_31095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8112236.1"
                     /translation="MARKIVLCRCEDVTLADLGHCVSRGYRDIEEVKRYTGFGTGPCQ
                     GKECLAAVASQLALLTDQPPAAIPPFTTRPPLAPTPLRLLARDPGAHRFADDPDSERS
                     S"
     gene            complement(2452..>2701)
                     /locus_tag="VF516_31100"
     CDS             complement(2452..>2701)
                     /locus_tag="VF516_31100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112237.1"
                     /translation="DIIAVAGIPAPSSDGPSQQGCKVVLDPPAGGFRVVVDEHGRTSA
                     PGGWACGDVTGYVGPAAAAEHGARVGMNVAMATMAVDW"
BASE COUNT          390 a          980 c          977 g          354 t
ORIGIN      
        1 cacggggcac cggcgccgcg gtcggcgccg gcttgatcac gcccgcgagc tggctcatcg
       61 cggtgcgcag ctccgcgtcg gacaccagca gctcgagcgt cgccgtcatg tcgcggccga
      121 cccggtccag gtagccggcg agccggcggt cctgcttctg caggtagttg ccgcgcgatc
      181 tgagccgggt ctcgttgcgg accacgaacg tgccgccgac gatcagcagc gcggcgagcg
      241 ccaggcagcc ggcgaggaac ttgaagaaca tcgggaaccg cagcttgcgg gcggtcatga
      301 catcctgcct tccgcgtccg cccgcgccag cggcgcaagc ggcggggcgg tgagaaccgc
      361 gcgcgcgtcg agcacgcgca cgctgccggt cggggtcgcg accatgcctg cgagcaggcc
      421 ggcgacctcg ccgggcaggt gggccggcgg cggctcgagc ggcggctcga ccgtccgcag
      481 atcgcgcagc cggtcgacca cgatccccgc cacgccggcc gcgcaatcca ccacgacgat
      541 cttgctgtcg tcgccggccg cggtcgccgg cagcccgagc agcacgccga gatcgatcac
      601 cggcacgatg tccccgcgca ggctgaacac gccggccagg cacggcgggg tcaggaacac
      661 ccgcgtgatc ggcggcaccg gcagggtctc gcgcacgtcg gcgatcggca gcgcgagctc
      721 ctgcccgcgc agctcgaagc acaccagacg cagcgggccg ccggccccgg gggacaccat
      781 ccgcccgagg ttacccgatg ttgaagtcct ctttttccgg aaccgggggc gctccgccgg
      841 agaaccggcc gagcgtgtag cgcgcgaaga tctcgtgggg ctcgccgccg gtcagccact
      901 cgccatacag cttgccgacg atcggcgcca tcatgaagcc gtggccgacg aagccacagg
      961 cgaggaagaa gtcggggtgg ccgggcgccg cgccgaggat cgggttgccg tcgggcgact
     1021 ggtcgtacgg cccggcccac tggcggagga ccttgatctc gccgaggatc ggcatcagcc
     1081 ggaccagccg gcgcgcgtag gtcgcgagga actccagcgt cgagcccatg ccgtacgtcg
     1141 agtcgtggcc ggggatggtg acgccgccga cgatctcgcc gcgcatcgac tggctgcagt
     1201 acagcccgct ggtcagctcg ctgaccatcg gtcgcaggaa cggcttgagc ggctccgacg
     1261 agcagatctc gtggcggatc gggtaggtcg ggacgtggac gccgatcatc tcggcgagcc
     1321 ggggcgacca cgcgccggtc gcgttgacca gccggcgcgc ccggaccgca ccgcgcgggg
     1381 tgtggaccag gtagccgccg cccttctgcg gctccagccc accgacgggg gtctgggtga
     1441 agatccggac gccgtgcgcc gcggcctggc gcgcgtagcc ccacaggaac ggccacggga
     1501 acaggatgcc gtcggtcggg ttgtacgccg cggcgatgat cccggtcagg tcgagctcgg
     1561 gcacgatgtc gcgcgccttg tcgggctcga gcatccgggt cggcacgccg cagcggttct
     1621 ggatcgcgat gttcttctcg agccgcgcga cctcctgggc gctgcgcgcg aggaacaggt
     1681 agccgccctg gcggaaccac acgttgacgc cgaggtcgac cgcgaaccgc cggcacagct
     1741 cgaccgactc ctgcatcagc cggatgttga gctcggtcga ccactgctgg cggaccccgc
     1801 cgccgttgcg ccccgacgcg ccctccgcga ggtagccgcg ctcgatcacg accacgtcgt
     1861 cgagcccgcg ccgggcgagg ttgtaggcga tcgccagccc gaccacgccg ccgccgacga
     1921 tcacgacctc ggcacggtcg ggcagcggcc cggcgccctc gagcgtggcg ggcagccgga
     1981 tctcgcccga cacgtcggcg cgggacgtgt cctcctcctc gtcgagcccc ggcggttgcg
     2041 tcggatgtcg cgtcggcact acgagctccg ctcgctgtcc gggtcatcgg cgaagcgatg
     2101 cgctccgggg tcgcgcgcga gcaggcgcag cggggtgggg gcgagcggcg gccgcgtggt
     2161 gaacggcggg atcgcggcgg gcggctggtc ggtcagcagc gcgagctgcg acgcgaccgc
     2221 ggcgaggcac tccttgccct ggcacggccc ggtgccgaac ccggtgtacc gcttcacctc
     2281 ctcgatgtcc cggtagccgc gcgaaacgca gtgtcccaga tcggcgagcg tgacatcctc
     2341 gcatcgacac agaacgatct ttcgggccac cccgggacga tagccaactc cgcgcggctt
     2401 gacatcgatt gtttacggga acttgaccgt gagacgtgtg caggacttgc actaccagtc
     2461 gacggccatg gtcgccatcg cgacgttcat ccccacccgc gcgccgtgct ccgcggcggc
     2521 ggcgggcccg acatagcctg tgacatcgcc gcacgcccat cctccgggcg ccgaggtccg
     2581 gccgtgctcg tcgaccacga cccggaaccc cccggcgggt ggatcgagca cgaccttgca
     2641 gccctgctgg ctcggcccat ccgaggacgg cgcagggatg cccgcgacgg cgattatgtc
     2701 g
//
LOCUS       DASVOR010001661         2521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_133806,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001661 DASVOR010000000
VERSION     DASVOR010001661.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133806"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..>2521)
                     /locus_tag="VF516_31105"
     CDS             complement(234..>2521)
                     /locus_tag="VF516_31105"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GGDEF domain-containing phosphodiesterase"
                     /protein_id="HEX8112238.1"
                     /translation="VRLGAQRAELSWSEAALVLTLAMVSGAWAVLITPIAVAANYAQR
                     RIAPMKTLYNIAINTVAATAAAGVLAIVKVSQPYDGSELLTLAAAGAVAGLVTHLAVA
                     AVVAAAQDLPLLATWRASAGLQLLTLAGNLALASAVLFLARYAIWMVAVLPLVVLCLH
                     QAYEGRLRGGQEREAGRRHAAAVGRLTEDLDEPGVLRRATEDACALADVDVVEIELPA
                     HDRAPALLHRHFRREPPWVGDPAEAPTLPARMVADMLVPTNDGADPGRLRAWLVGGAS
                     DLHLGRFEEDALQSLAAHAGAAVRNARIHARQTFLATHDRLTELPTRQVLIDHVEKRF
                     RTGADASPTALIVLDLIGYRDILRALGHDVAEDLLAQTASRLWQAADSGEYVAHVGMD
                     DFGVYLPTANDPAHVRGRALAFLSAITRPIELGTSDPETTHVTLNATAGAAYSIIPIG
                     SGAELLRQASVALNQARAENINFEFYNPAMDELGGPAAVVLNSELSAAIKNDQLDLHY
                     QPIIDLPSGAPLAMEAMLRWSHPTKGLLRVGEFASVLERSPDHPRFVAWQLDRALNAR
                     RGWGDRQLPVSVNLASRCLLDHRFPEQVFAALDRANLPPHQLMFEIDETAVLSHLGLV
                     GDVLTVLRLRGVQIAIDNLGTGSSSLFSLLRVPATHVKVDGHYVRDMLVDPEAMAVVC
                     LGLDLSRRADLQFIATGVNSPELITALRQRGCDAAQGPYFTRPLIADEVLAYLATAPE
                     ISDVPDGSVVALDTRRHTPVP"
BASE COUNT          448 a          809 c          849 g          415 t
ORIGIN      
        1 gagcccgcag ccggtcgctg gacacgacga cgtccgccgg gaactgagcc gccgcccacg
       61 tcgacttccc ggacgcgcca ggacccacga gcacgacaac acacggcgcc ggcaggttca
      121 ccgacccagt gtgccgcgcc acctccgccg tcggagcggc gttgcacagg ccatgtcatc
      181 tgctgagctg tggccgcccc tgccgccgtc ccggcctccg ccgggcggat ccgtcacggg
      241 accggggtat gtctgcgcgt atcgagcgcc acgacggagc cgtccggcac atctgagatc
      301 tccggcgcag tagcgaggta ggcaagcacc tcgtcggcga tcagtggtcg ggtgaagtac
      361 ggaccctggg ccgcgtcgca tccgcgctgc ctcagcgcgg tgatcagctc cggactgttg
      421 acgccggtcg cgatgaactg gaggtcagca cggcgactga ggtccaggcc gagacagacg
      481 acggccatgg cctcggggtc gacgagcatg tcgcgcacgt agtgcccgtc caccttgacg
      541 tgcgtggccg gtactcggag gaggctgaag agcgaactgc tgcccgttcc caggttgtcg
      601 atcgcgatct gtacgccgcg tagccgcagc acggtcagca cgtcgccgac cagcccgaga
      661 tggctcagca cagccgtctc gtcgatctcg aacatcagct ggtggggcgg caggttcgcg
      721 cgatcgagcg cagcgaagac ctgttccggg aaccgatgat ccaggaggca ccggctcgcg
      781 aggttgacgc tgaccgggag ctgccgatct ccccaccctc gccgggcatt gagcgcgcgg
      841 tcgagttgcc aagcgacgaa gcgtggatgg tcaggtgacc gctcgaggac cgatgcgaac
      901 tctccgaccc ggagcaggcc cttcgtcggg tgggaccagc gcagcatcgc ttccatcgcg
      961 agcggcgcgc cggagggaag gtcgatgatc ggttggtagt gcaggtccag ctggtcgttc
     1021 ttgatcgctg cgctcagctc ggagttgaga acgaccgcgg ccgggccgcc gagctcgtcc
     1081 atagccgggt tgtagaactc gaagttgatg ttctcggcgc gcgcttggtt gagggcgacc
     1141 gaggcctggc ggaggagttc tgcaccgctt ccgatcggta ttatcgagta ggcggcgccg
     1201 gcggtggcgt tgagagttac gtgcgtggtc tcggggtcgg acgtgccgag ttcgatcggc
     1261 cgcgtgatcg cgctcagaaa ggcaagcgcg cgaccgcgta cgtgggcggg atcattcgca
     1321 gtaggcaggt acactccgaa gtcgtccatg ccgacgtgcg caacgtattc gccactgtcg
     1381 gccgcttgcc acagtcggct cgccgtctgt gccagcaggt cttcggcgac atcgtggccg
     1441 agtgcgcgga ggatgtctcg atagccgatg aggtcgagca cgatcaacgc agtggggctc
     1501 gcgtctgcac cagtacgaaa tcgtttctcg acgtggtcga tcaatacttg ccgagtgggc
     1561 agctccgtca aacggtcatg agttgcaaga aaagtctgcc gagcgtggat gcgcgcattc
     1621 cgcactgcgg ccccggcatg tgccgcgaga ctctgcagcg cgtcttcttc gaaccgaccg
     1681 agatggaggt cggaggctcc gccgacgagc cacgcacgga gtcgtccagg gtcggcgccg
     1741 tcgttcgtcg gcaccagcat gtcggcgacc atgcgagccg gcagggtggg tgcttcggcg
     1801 ggatcgccga cccagggtgg ctcgcgccgg aagtgccgat gcagcaaggc cggagcgcgg
     1861 tcgtgcgctg gaagctcgat ctccacgacg tccacgtcag cgagtgcgca agcgtcctcg
     1921 gtcgcgcgcc tcagaactcc tggctcatca aggtcctcgg tcaagcggcc aacagcagca
     1981 gcatgccgcc gccccgcctc ccgctcctga cccccacgga gtcgcccctc ataggcctga
     2041 tgtaggcaca gaacgacgag cggtagaacg gcgaccatcc atattgcata acgggcaaga
     2101 aagagcacag cggaagccag cgccaggttt ccggctagag tcagtaactg caatccagcg
     2161 ctcgctcgcc atgtggccaa tagaggaaga tcctgggctg cagcgacaac cgcagcgacc
     2221 gcgaggtgag taacgagtcc ggcgaccgcg cctgcggcgg caagcgtcaa cagctcagag
     2281 ccatcatacg gttgggagac tttgacgatc gctagaactc ccgctgcggc cgtggcggcc
     2341 actgtattga ttgctatatt atacaaagtc ttcatcggcg cgatgcgtcg ttgcgcataa
     2401 ttcgcggcga cggcaattgg tgttatcagt accgcccatg cacccgacac cattgccagc
     2461 gtcagtacga gcgcggcctc gctccacgac aactctgccc gttgcgcgcc cagtcggaca
     2521 g
//
LOCUS       DASVOR010001662         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_162969,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001662 DASVOR010000000
VERSION     DASVOR010001662.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162969"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1680
                     /locus_tag="VF516_31110"
     CDS             <1..>1680
                     /locus_tag="VF516_31110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112239.1"
                     /translation="VLALARPEVFEVFPRLWAERQNVQEIRLQELGRKAGERLVRQVL
                     GDHIGPETLERLVRHAGGNAFYLEELIRAAAEGKDHALPDTVLAMVETRLGRLTVEAR
                     RVLRAASVFGEVCWEGGVAVLLAGAMTAAAVADWLSRLAEQELLAIRPHSRVPGEREL
                     AFRHALLREGTYATLTEDDKRLGHRLAGTWLADHGESDPMVLAGHFERGGEGERAAEF
                     YLRAAEQAQYAAADVDATMARVRLGLACAPSRDLRIRLLGIRCEVSTFNLENINATIA
                     DAEALAREAPRGSIPWVQGVMPYNYGMLVAGRIDELLASLAALREVDPTPDAVAKMAY
                     ALLLGICAVDNLGRVADGTALEKRCVAIIPPTADRDPLAHVWWNIAIGMRASYAHDDP
                     WAGLQHASAIQAIFRVTSGELIFLNMQLFRALNFWYLGELTEAELVHESIVPADETLG
                     MVASLRRFSLAWLRADRGELDAAHATAAQLSQHGQAIRNTLDEGRGRWVLAEVLRRKG
                     DLSGAERELGVALGMTIPLEYPGALGTLSALRLAQGRPAEALAVAEDAMAR"
BASE COUNT          225 a          610 c          619 g          226 t
ORIGIN      
        1 tggtgctcgc gctcgcccgc cccgaggtgt tcgaggtgtt ccccaggctg tgggccgagc
       61 gccagaacgt ccaggagatc cgcctccagg agctcggccg caaggccggc gagcggctgg
      121 tccgccaggt gctcggcgac cacatcggcc ccgagaccct ggagcgcctg gtccggcacg
      181 ccggcggcaa cgcgttctac ctcgaggagc tgatccgcgc cgccgccgag ggcaaggacc
      241 acgcgctgcc cgacaccgtg ctggcgatgg tcgagacccg gctcggccgg ctcacggtcg
      301 aggcccgccg cgtgctgcgc gccgccagcg tgttcggcga ggtgtgctgg gagggcggcg
      361 tcgccgtcct gctcgcgggc gcgatgaccg cggcggcggt cgccgactgg ctgtcacggc
      421 tggccgagca ggagctgctg gcgatccgcc cgcacagccg ggtccccggc gagcgcgagc
      481 tcgcgttccg ccacgccctg ctgcgcgagg gcacctacgc gaccctgacc gaggacgaca
      541 agcgactcgg ccaccggctc gccggaacgt ggctggcgga ccacggcgaa agcgatccga
      601 tggtgctcgc cggccacttc gagcgcggcg gcgaaggcga gcgcgccgcg gagttctacc
      661 tgcgcgccgc cgagcaggcg cagtacgccg ccgccgacgt cgacgcgacg atggcgcgcg
      721 tccggctcgg cctggcctgc gcaccatcgc gggacctccg gatccggctg ctcggcatcc
      781 gatgcgaggt gtccaccttc aacctggaga acatcaacgc cacgatcgcc gacgccgagg
      841 cgctggcgcg cgaagcgccg cgcggctcga tcccctgggt ccagggcgtg atgccgtaca
      901 actacggcat gctcgtcgcc ggcaggatcg acgagctgct ggcgtcgctc gccgcgctcc
      961 gcgaggtcga tcccacgccc gacgcggtgg cgaagatggc ctacgccctc ctcctgggga
     1021 tctgcgccgt cgacaacctc gggcgcgttg ccgacgggac cgcgctggag aagcggtgcg
     1081 tcgcgatcat ccccccgacc gcggatcgcg acccgctcgc gcatgtgtgg tggaacatcg
     1141 cgatcggcat gcgggcctcg tacgcgcacg acgacccgtg ggccggcctg caacacgcca
     1201 gcgcgatcca ggcgatcttc cgggtgacca gcggtgagct gatcttcctg aacatgcagc
     1261 tgtttcgcgc gctgaacttc tggtacctgg gcgagctcac cgaggccgag ctcgttcacg
     1321 aatcgatcgt gccggcggac gagacgctgg gcatggtggc gtcgctgcgg cgcttcagcc
     1381 tcgcctggct gcgcgccgat cgcggcgagc tggacgcggc acatgccacg gcggcccagc
     1441 tgagtcagca tggccaggcg atccgcaaca cgctggacga ggggcgcggc cgctgggtgc
     1501 tcgcggaggt gctccgccgc aagggcgatc tctcgggcgc ggagcgcgag ctgggggtcg
     1561 cgctcgggat gaccatcccg ctggagtatc caggtgccct cgggacgctg tcggcgctgc
     1621 gcctcgccca gggccgcccc gccgaggcgc tcgcggtggc cgaggatgcc atggcgcgct
//
LOCUS       DASVOR010001663         5961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_543324,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001663 DASVOR010000000
VERSION     DASVOR010001663.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5961
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543324"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="VF516_31115"
     CDS             <1..690
                     /locus_tag="VF516_31115"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8112240.1"
                     /translation="ESFLDELAAAAGQDPLAYRLALIDQPPPLGPPDPDRAVFSPQRM
                     KDCLTLAADRAGWGKPLPAGHGLGLACGGFDHRSYAAIVVEASAAAGRIAVHRVVCAF
                     DCATVVHPDGARAQVEGAITQGLSAALHERMTIEGGRAVDTNFHAHPLLRLHEAPARI
                     EVHFVDRPDAQISGLGESALPPVAPALANALYRATGHRPPAARAAAPAAGVAGGPARR
                     SGVEVWEEYKE"
     gene            complement(828..1259)
                     /locus_tag="VF516_31120"
     CDS             complement(828..1259)
                     /locus_tag="VF516_31120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112241.1"
                     /translation="MPESPEPEPALNATAQASTTDTVMADQDAQDRAEAAFLDALRTY
                     QAGHETDITLGGHCNVRLTECDPYPIGKGKHPRVDAICSQVCLDTIAHCEDYTTEELE
                     FCCANPGKPFRDRAGKVYPVRVCMPSGEPNWNKRCAPGLNP"
     gene            complement(1511..1909)
                     /locus_tag="VF516_31125"
     CDS             complement(1511..1909)
                     /locus_tag="VF516_31125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112242.1"
                     /translation="MQGFTISAARNINTALGDGVRRRRGRVFADRYHVEVITTPTRAR
                     HALGYILGNWRKHGEDRHGLASTWLVDPFSSGIVFPDWKELEDQPWMWPMRETYDPLM
                     VRRPQTWLLREGWKLGGGPISARDVPSKLR"
     gene            2750..3061
                     /locus_tag="VF516_31130"
     CDS             2750..3061
                     /locus_tag="VF516_31130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112243.1"
                     /translation="MKAHRSTQVRVSNDPQNSNFEAGTALENLRAEMIDIEALARAAE
                     AAADALPSPANDRQRLLFGRIQSLATKTSHQASASLRFANAQVTALTAQMEARRGAAG
                     H"
     gene            4098..4268
                     /locus_tag="VF516_31135"
     CDS             4098..4268
                     /locus_tag="VF516_31135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112244.1"
                     /translation="MPGRHVESIAIIQLRVTWGTRKVQLAARFSGAVFLGVAFLAVLG
                     VAALDGWRWLGH"
     gene            complement(4279..5070)
                     /locus_tag="VF516_31140"
     CDS             complement(4279..5070)
                     /locus_tag="VF516_31140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112245.1"
                     /translation="MAAFGKPVALTSLDTSADEEHADLSADELTIYFSSTRPGGAGGF
                     DIYQATRRSTAEPFGNVLPVAGVNTAGDERVPRMASNGLSMYAMTATSAAPALHIALA
                     SRANVTVTFTGLKMVANINNATNTADPYLVSSGNVMYLASDLAGNLGLYRSAKVNGAF
                     STPTPVPGVNLDTPAIENNPVVTPDELTLFFGSTRPGGTGDYDIYEARRAHVADGFGA
                     PVELKGLDSPGADVPNWISPDGCNLYFTRLEANVGYQLYMASRGH"
     gene            complement(5395..>5961)
                     /locus_tag="VF516_31145"
     CDS             complement(5395..>5961)
                     /locus_tag="VF516_31145"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX8112246.1"
                     /translation="LPLPWRPALGGLSCGAILAVLPLVAGNGFEPLDALLDGKLAFGM
                     VALLLLAKPIGTAFAVGSGNPGGAFTPTLLIGGCVGALYAWGLHAVFGGAIGAPSTYA
                     LIGLAAAMAATTHAPLTGAVLACELSGDYALMLPLLVATALAAAWARRLYVDSVYTVE
                     LSHRGLRWRLTLDGRRVIESQQHTVDVV"
BASE COUNT          959 a         2022 c         2018 g          962 t
ORIGIN      
        1 gagagcttcc tcgacgagct cgccgcggcg gcgggccagg acccgctcgc ctaccgcctt
       61 gcgctgatcg accagccacc gccgctcggg ccgcccgatc cggaccgcgc ggtgttctcg
      121 ccccagcgca tgaaggactg cttgaccctc gccgccgacc gcgccggctg gggcaagccg
      181 ctgcccgccg gccacggcct gggcctggcg tgcggcggct tcgatcaccg gagctacgcg
      241 gcgatcgtcg tcgaggcctc ggccgccgcc ggccggatcg ccgtgcaccg cgtggtgtgc
      301 gcgttcgact gcgcgaccgt ggtccacccc gatggcgcgc gcgcccaggt cgagggagcg
      361 atcacccagg gcctgtccgc cgcgctccac gagcggatga ccatcgaggg cggccgcgcg
      421 gtcgacacca acttccacgc ccacccgctg ctgcggctgc acgaggcgcc ggcgcgcatc
      481 gaggtccact tcgtcgaccg ccccgacgcg cagatcagcg gcctcggcga gtccgcgctg
      541 cctccggtcg ccccggcgct ggccaacgcg ctctaccgcg ccaccggcca ccggccaccg
      601 gctgcgcgag ctgccgctcc ggctgccggg gtagccggag gtccggccag gcgcagcggc
      661 gtggaagtgt gggaggagta caaggaatag cgtgatgcaa tgctgaccgt gaacggagcc
      721 gcgggaggac gatgcacaag gtcggtgtcg agctcggcgg tagcttcatg gaagcctgct
      781 ggtcacggcg tgcgcgtggt gtcgagcgga attcgcccac cactggttca ggggttcagg
      841 ccgggcgcgc agcgcttgtt ccagttcggc tcgccgctcg gcatgcacac gcggactggg
      901 tacaccttgc cggcgcggtc gcggaacggc ttgcccgggt tcgcgcagca gaactcgagc
      961 tcctcggtcg tgtagtcctc gcagtgggcg atggtgtcga ggcagacctg cgagcagatc
     1021 gcgtccacgc gcgggtgctt gcccttgccg atcggatacg gatcgcattc ggtcaggcgc
     1081 acgttgcagt gaccgccgag ggtgatgtca gtctcgtggc ccgcctggta ggtgcgcagc
     1141 gcgtcgagga aagcggcctc tgcgcgatcc tgggcgtcct ggtcggccat gacggtgtcg
     1201 gtggtggatg cctgggcggt ggcgttgagc gcgggctcgg gctcgggtga ctcgggcatc
     1261 gagaggcacg aagctatcag ggcgatggat aggagcaggg acggcttgag gtcgcgttga
     1321 gcagtcagca ttttaccgag atacagcgct cgcgcggtcc gtgggctgag cgttgggttg
     1381 gtcgatcgtg aaggcggggg gcgtgctccg atgtcgcgtg cgcctctgtc agtccgcgcg
     1441 ctactccagc tctcgctgat cggtttcgat ctgtaatccg cgccattcgc cgtctgccag
     1501 ccgtgcgcgt tcaacgcagc ttactcggca catcgcgcgc gctgatcgga ccaccaccga
     1561 gcttccagcc ctcgcgcaat aaccacgtct gcggccggcg aaccatcagc gggtcgtagg
     1621 tctcgcgcat cggccacatc cagggctggt cctccagctc cttccaatcc gggaacacga
     1681 tcccggatga gaagggatcg acgagccagg tgctcgcgag gccatggcgg tcctcgccat
     1741 gctttcgcca gtttcctaaa atgtaaccga gagcatgacg cgcccttgtc ggtgtcgtga
     1801 tcacctcgac gtgatagcga tcggcaaaga ccctgccgcg gcgccggcgt acgccatccc
     1861 cgagcgcggt gttgatgttc ctcgccgccg agatcgtgaa gccctgcatc ccgcgagcaa
     1921 gcgctgcctt gtgatcgtgg cctcgcgcag cgccttgtac atcgcccgcc gccgcaggct
     1981 gccgacctcg ggcgccaccc gcatcacgat atgcagcgca tgataccgct tgaactccgg
     2041 ccgcgcctcg tgccgctcgc cggcccgtgc acccttcggc tttcgcccgg cgccgcgccg
     2101 cttgccgccg cgtcgaaaca gctcctgctg cgtgccccgc ctcggcttgc acttcgacct
     2161 cgacctcatg cacgcatcgt taccacgaac atgcagcctg tcaatgattg agcggggcta
     2221 attattactc attctcgtgg attgaacgct ggcacagccc ttgttgaaaa catcctgatc
     2281 aagtcacttg tctccgaaat catacagacg aatcgccagt ctcctgcaga gcgatgagct
     2341 cttcttgacg tcgagagtgg cagcggtcgc cggacatgag gtcgacgaat cctcgagcga
     2401 aaccctctgc agagccatcg gacggccggt ggagaactgg ggcgtctgat gacacgcgct
     2461 gcagcacgcc agatcgaccc ggacacgtct cacagtcgcc cttcagttcc ccacagtcat
     2521 tgccctcgac tccaggctcc gttcttgcat tttaatatat tcaggattcc ggacacgaag
     2581 agaatctggc gacagatcca ccacctcctg cactccacca tggactcacc tcgctgtccc
     2641 ggcaatatgg aagcgttccc atccggaacg aggcggcgcc gccacaggcg caggtcagat
     2701 ccatagcgac ccgccggtgt tagcatcacc gacgggtcca ggagccacga tgaaggcaca
     2761 tcgcagcacc caggtcaggg tatccaacga tccgcagaac agcaacttcg aggccggtac
     2821 ggcgctggag aacctccgcg cggagatgat cgacatcgag gcgctcgcgc gcgccgcgga
     2881 ggccgccgca gacgccctgc cgtcgccagc gaacgatcgc cagcgcctgc tgttcggccg
     2941 gatccagtcg ctcgcgacca agacctcgca ccaggccagc gcctcgctcc gcttcgcaaa
     3001 tgcgcaggtc accgcgctca cggcgcagat ggaggcgcgc cgaggcgccg ccggtcattg
     3061 atgtccccgc tgggaccgga cgtctcgcgt gaactgaaag tcccgactgg accagagtct
     3121 cgcggaacca ggagtcccgg ctgggaccga gcctttcgtc ggaacccgag tcccagctgg
     3181 ggccgaatgc tcgcgggatc cggaagtccc agctgggacc gaatgctcgc gggatccgga
     3241 agcctcgcgg aagctgaagc ctcgcgggtt cggaaccctc gaggaacccg gaagtgccag
     3301 ctgggaccgg aaccgctcca cagaggggcc gtccggaaac gacaggaacc ggtcggcgcg
     3361 tttgagccga agccacgctg cccgtcgttg cggcacccga gtgagccctt ctccagcgat
     3421 ccggaacgag atcaaataaa tccgaaaggg agcatccgcg cggggcgatg gattgtgagt
     3481 ggaccgccgt ggtccgcttg atcggctgtg gcgggccagt cacaggcccg tccacgcggt
     3541 ctcccgggtc cctggccgta gccgcgtgcg atgatcctcg tggtccctcg ttggctactc
     3601 ggccgcatgc agtcgctcgc cacgaagaac tcgcagcagc tcgccgtcgt ccgctttccg
     3661 gtcgcacggg tgatgcacca tgagtgggcc ttcctgaatc cgcctgatca gtttcgacgg
     3721 accagtgaca ggccgtccgc gcggtctcca gggtccctgg ccgcagccgc gtgcgatgtt
     3781 catgttcggc gacgatgatt tctgccggca cgaatgattg gcgttatcag ttcatgacgt
     3841 attcacgatg tagcataata gtgcaccccg aggggccgtg ctgttggaga aatccagccg
     3901 cttgcgagcg tctcaccccg cgtgcgaacg cgcagatcgc gggttagcac tgccaagcca
     3961 gtggataatt ctgcaagggg ttagagaggc gtgcacaggc ccagatcgct atcggccacc
     4021 tgctggaggt gtgtcatcag gttggacgag cacgcgacga cgggacggat cccgaccccg
     4081 cgtgaaggag gagcgccgtg ccgggtcgcc atgtcgaaag catcgccatc atccagctca
     4141 gggtcacgtg gggcacccgc aaggtgcagc tggccgcccg gttcagcgga gccgtcttcc
     4201 tcggggtcgc gtttctggcc gtcctgggcg tcgccgcgct cgacggatgg cgctggcttg
     4261 gtcactgacg tagtcgcgct agtgtccccg gctcgccatg tagagctgat aaccgacgtt
     4321 cgcttccagc cgcgtgaagt acaggttgca gccatcgggc gagatccagt tggggacgtc
     4381 ggccccggga ctgtcgaggc ccttgagctc gaccggcgcg ccgaagccat cggcgacgtg
     4441 cgcgcggcgt gcctcgtaga tgtcgtagtc gccggtgccg cccggacggg tcgagccgaa
     4501 gaacagcgtg agctcgtccg gcgtcaccac cgggttgttc tcgatcgccg gcgtgtcgag
     4561 gttgacgccg gggaccggcg tcggggtgga gaacgcgccg ttgacctttg cgctgcgata
     4621 caggccgaga ttgccggcga ggtccgatgc gaggtacatg acgttgccgc tcgacacgag
     4681 gtacggatcg gcggtgttgg tcgcgttgtt gatgttggcg accatcttca gcccggtgaa
     4741 cgtgacggtg acgttggcgc ggctggccag ggcgatgtgg agcgccggag cggcggaggt
     4801 cgcggtcatc gcgtacatcg acaggccgtt gctggccatc cgtggcacac gctcgtcgcc
     4861 cgcggtgttg acgccggcga ccggcaggac gttgccaaac ggctcggcgg tcgagcggcg
     4921 ggtcgcctgg tagatgtcga agccgccggc gccaccgggg cgcgtgctgg agaagtagat
     4981 cgtcagctcg tccgcggaca gatcggcgtg ctcctcatcg gcgctggtgt cgagcgaggt
     5041 cagcgcgacc ggcttgccga acgcggccac cggattgcac cgcgccggcc gggcgtctcc
     5101 agcaccatcg ctggccgcat ccgtcggacc agcagtgctc gcgacctgtc cacatccggc
     5161 gagagctgcc aggaccgctc ccgtctccca tgcgcgccgt gcattcccca cgtcgcaacg
     5221 ggctaccatg atcccgcgcg cgccgatggc ccacgatcgg aggttgccgc agcttgacga
     5281 cggtgcgctc gccccgcaca tggcagtgtg cgcccgcgcc cggccgctgt ttgccaatca
     5341 tcgtgagcgc cgatcatcgt gagtcgatca tcatgaatcg atcacacgaa ccgatcacac
     5401 cacgtcgacg gtgtgctgct ggctctcgat cacgcggcgg ccgtcgaggg tgagccgcca
     5461 gcgcaggccg cggtgcgaca gctccacggt gtagaccgag tcgacgtaga gccggcgggc
     5521 ccacgccgcg gcgagcgcgg tcgcgaccag cagcggcagc atcagcgcgt agtcgccgga
     5581 cagctcgcac gccagcaccg cgccggtcag cggcgcgtgg gtggtggcgg ccatcgccgc
     5641 ggcgaggccg atcagcgcgt aggtcgacgg cgcgccgatc gcgccgccga acacggcgtg
     5701 caggccccag gcgtacagcg cgccgacgca gccgccgatc agcagcgtcg gagtgaacgc
     5761 gccgcccggg ttgcccgagc cgaccgcgaa cgcggtgccg atcggcttgg ccaggagcaa
     5821 cagcgccacc atcccgaacg cgagcttgcc gtcgagcagc gcgtcgagcg gctcgaagcc
     5881 gttgccggcg accagcggca acaccgcgag gatcgcgccg cacgaaaggc cgccgagcgc
     5941 cggccgccac ggcagcggca g
//
LOCUS       DASVOR010001664         7754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_425544,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001664 DASVOR010000000
VERSION     DASVOR010001664.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425544"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1478
                     /locus_tag="VF516_31150"
     CDS             <1..1478
                     /locus_tag="VF516_31150"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX8112247.1"
                     /translation="PYFADARPRSVLCMPVRLRADSVALLYLENELVPGTSTPERLAA
                     LELLAAQAAISLENARLLERERTGRIEAEAAEHRALLLGEATALLSQTLESRGVLDAL
                     ARLFTRSFADFAVIDLQDSGALVRIACAHRDPDQEPILREAAARYPAQPRSPVWQVLH
                     TGQAVEAPVLTDEQIQTYCVDDHHAELARRLGARSGVFVPLCARHAVIGVLSLAAVTP
                     NRFPRADVELAVDLGRRMALAIDNARLLDETRRALHLRDEFLRIASHELRTPLASLRL
                     SAQGLLRAAERQRTVSPEILDRTLHRVLGNTARLEQLTSELLDVTRIEQGRLQLNPAE
                     IALDAIVREAVEHIEADLAAAGSSLSIECSAPVVGRWDPSRLDQVVTNLLTNAAKFGA
                     GKPIEIRIDRLGDTARLTVIDHGIGIDPARRPYGFDRVERAVPASRYGGLGLGLYIAR
                     SIVVAHGGTITVDSELGAGSTFTVTLPCSTPEPGAAQPVSP"
     gene            complement(1493..3229)
                     /locus_tag="VF516_31155"
     CDS             complement(1493..3229)
                     /locus_tag="VF516_31155"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HEX8112248.1"
                     /translation="MHRRSYVLLVPALLYVAALSPRRALADVRSHPAAAVHCGELATD
                     PRHGLAGNPVIKSVTSQIIAVSGANASYCQVDILFGTNPAQNINIRVGLPLDAADGGT
                     GGVQGAWNGRTQGLGGGGCAGNLIVTPAVNTGYAGSGTDLGHAGGNCEPGVNADGTYN
                     TQFIEDFIRNAIKEQVLLSKRVADAYYGMKPAFNYWNGCSTGGRQGYLLAQELAGELD
                     GILASAPAIYWTRFQTAQMWGEIAMKELAGGPISAAKLNQATASAVAACDAADGVTDG
                     VVDDPRTCTFSATANICGAPTAPATNCLTAAEPEAIDRIWDGPRNADGNKIWFGLDRG
                     TTFLGLNGPLPFFLGVTQFHWDEHDLGFDWRTVGVDGYPQVAQDGSRNIADVTDTFGN
                     LDAFRRSGGKLLTFVGGNDQLIYPRGVLNYYRGMAARYAHRDGDFDGVQRFYRLFRAP
                     GVGHCGGGNGPQPQNLFGALVDWVEHGVAPQQILATTTVGGAVTRSRPLCPYPQTAIY
                     NGSGSTDDAASFHCGGNLEQPHVVCRDALVTYKHEVDGRVDLQGSGLDHGVCRRAGHH
                     GDGDGDSGDHPR"
     gene            3394..4953
                     /locus_tag="VF516_31160"
     CDS             3394..4953
                     /locus_tag="VF516_31160"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8112249.1"
                     /translation="MRRLRLLPAVAVSAGLAGLAALAGLAGPALAQAPAPLPPAAAQK
                     LAQGTFREYLELLALPNDATRGADIQRNVEFLERAFRKRGFTTRRLDNGERPLLYAEW
                     PTQPAGAKTILYYMHLDGQPVVDREWSQPSPWQPVVKQKNRAGGWDVVPSDTLFAPAL
                     DPELRVFARSASDDKAPIMMFLAAFDGLRQAGAAPANHVKVLIDSEEEKGSPSIRAVV
                     TANKDLLRCDALIIQDGPRHASERPTLVFGNRGAAHVVLTVYGPRQPLHSGHFGNYVP
                     NPAIRLARLLASMKDDDGRVTVAGYYDRVQLTAAEKQVLAETGDDEPAIRRRVGIRSA
                     ERVGASLQEALQFPSLNVRGMAAASVGDKASNIVPHQATADIDIRTTPETPPDYLFGL
                     LQKHVEAQGYHLVPGPPTDEDRASYDKLATLAMDIGSRAVRTPLDAPLGTWAYAALQQ
                     ASQRGAPVRIRMMGGSVPTDSLVEVLGAPFVIIPLVNGDNNQHTFDENMRVGHYVEGI
                     RAMLAMLRRKF"
     gene            5911..6663
                     /locus_tag="VF516_31165"
     CDS             5911..6663
                     /locus_tag="VF516_31165"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8112250.1"
                     /translation="MAAGSPGRKRKLQQPQLVLFKKRGGKRRGAGRPPKGARAGSPHK
                     PRPYLHARYPVHVTLRVVGAVGNLRRRCVYQAIREATLTTARREDFRIVHLSIQRTHV
                     HLLVEANDKAALARGMQGFQISAAKHLNAAISKGKPGARRRGVVFPDRYHAEIITSPR
                     QVRHSLAYCLNNWRRHQEDRRAPMSGWTIDWFSTAVMFPGWVEYGDEPFLWRGPATYD
                     PMVVYQPRTWLLREGWKKAGAVSCHEVPAAGR"
     gene            complement(6700..>7754)
                     /locus_tag="VF516_31170"
     CDS             complement(6700..>7754)
                     /locus_tag="VF516_31170"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112251.1"
                     /translation="GYAHLVQLERDEGPDYTGRPEGASRKYSVQELLEGVRDDSSHRR
                     ARVSQATSEAIGRSPYHGKIDFGIVTIREDENEAVLRRFDKHAVEVQKRRYRIRRLKL
                     PGGGAYTIAVLRSLEQGNTGSLMAARDLLDDLSPGFVLVVGIAGGAPSSEFSLGDVVI
                     SSRIVDMSVEAVKDSGERTYAHGGGPLDPEAAGLAADIKAMIADGELDGWNTDTAIGK
                     PRPLVDLSPSKFYGTKASKAKLQADLERHFGGAPRAPLAVTGAISSSDRLIKDTALLN
                     RWQESARQVIAVEMESAGVYKATHDRVPFLAIRGVSDVVGYERHPDWTAYACETAAAF
                     TRAFLLARPTKPIEAR"
BASE COUNT         1145 a         2765 c         2631 g         1213 t
ORIGIN      
        1 atccgtactt cgccgacgcc cgcccgcgct cggtgctgtg catgccggtc cggttgcggg
       61 cggattcggt cgcgctgctc tacctcgaga acgagctggt cccgggcacg tccaccccgg
      121 agcggctggc cgcgctcgag ctgctggcgg cgcaggcggc gatctcgctc gagaacgccc
      181 ggctgctcga acgcgagcgc accgggcgga tcgaggccga ggccgccgag caccgcgcgc
      241 tgctgctcgg cgaggcgacc gcgctcctgt cccagacgct cgagtcgcgg ggtgtgctcg
      301 acgcgctggc ccggctgttc acgcggagct tcgcggactt tgcggtgatc gatctccagg
      361 acagcggcgc gctggtgcgg atcgcctgcg cgcaccgcga tcccgaccag gagccgatcc
      421 tccgcgaggc cgcggcgcgc tacccggcgc aacccaggtc cccggtgtgg caggtcctgc
      481 acaccggcca ggcggtcgag gctccggtcc tgaccgacga acagatccag acctactgcg
      541 tcgacgacca ccacgccgag ctcgcccgcc ggctcggcgc gcggagcggg gtgttcgtgc
      601 cgctgtgcgc gcggcatgcg gtgatcggtg tgctgtcgct ggccgcggtg acgccgaacc
      661 ggttcccacg agccgacgtc gagctcgcgg tcgatctcgg ccgccggatg gcgctggcga
      721 tcgacaacgc gcggttactc gacgagaccc ggcgcgcgct gcacctgcgc gacgagttcc
      781 tgcggatcgc gtcgcacgag ctccgcaccc cgctcgcgtc gctccggctc agcgcgcagg
      841 ggctgctgcg cgccgccgag cgccaacgca cggtgtcgcc ggagatcctg gaccgcaccc
      901 tccaccgcgt gctcggcaac accgctcgcc tcgagcagct cacgtcggag ctgctcgacg
      961 tcacccggat cgagcagggc cgcctccagc tcaacccggc cgagatcgcg ctcgacgcga
     1021 tcgtgcgcga ggcggtggag cacatcgagg ccgatctcgc ggcggcggga tcctcgctgt
     1081 cgatcgagtg ttcggcgccg gtcgtcggcc ggtgggatcc gtcgcggctc gatcaggtgg
     1141 tgaccaacct cctgaccaac gcggccaagt tcggtgccgg caagccgatc gagatccgga
     1201 tcgatcggct cggcgacacc gcgcggctga ccgtgatcga ccacggcatc ggcatcgatc
     1261 cggcgcgccg gccctacggg ttcgatcgcg tcgagcgcgc ggtcccggcg tcgcgctacg
     1321 gcgggctcgg gctggggctg tacatcgccc ggtcgatcgt cgtcgcccac ggcggcacga
     1381 tcaccgtcga cagcgagctg ggcgccggct ccacgttcac cgtgaccctg ccgtgctcca
     1441 cccccgaacc gggcgcggcg cagccggtct cgccctgatc gtcgcgcagc aatcagcggg
     1501 gatgatcgcc gctgtcgccg tccccgtccc cgtggtgccc ggcgcgccgg cagaccccgt
     1561 ggtcgaggcc gctgccctgg agatcgacgc ggccgtcgac ctcgtgcttg tacgtgacca
     1621 gcgcgtcccg gcacaccacg tggggctgct cgaggttgcc gccgcagtgg aagctggcgg
     1681 cgtcgtccgt gctgccgctg ccgttgtaga tcgcggtctg ggggtacggg cacagcgggc
     1741 ggctgcgcgt gaccgcgccg ccgaccgtgg tcgtggcgag gatctgctgc ggcgccaccc
     1801 cgtgctccac ccagtccacc agcgcgccga acaggttctg cggctgcggg ccgttgccgc
     1861 cgccgcagtg gccgacgccg ggcgcgcgga acaggcgata gaaccgctgc acgccgtcga
     1921 agtcgccgtc gcgatgcgcg tagcgcgccg ccatcccgcg gtagtagttg aggacgccgc
     1981 ggggatagat cagctgatcg ttgccgccga cgaacgtgag cagcttgccg cccgagcgcc
     2041 ggaacgcgtc gaggttgccg aacgtgtcgg tgacgtcggc gatgttgcgc gacccgtcct
     2101 gcgcgacctg cgggtagccg tcgacaccga ccgtccgcca gtcgaagccc aggtcgtgct
     2161 cgtcccagtg aaactgggtg acgccgagaa agaacggcag cgggccgttc aggccgagga
     2221 acgtcgtgcc gcggtcgagg ccgaaccaga tcttgttgcc gtcggcgttg cgcggcccgt
     2281 cccagatccg gtcgatcgcc tcgggctcgg cggcggtcag gcagttggtc gccggcgcgg
     2341 tcggtgcgcc gcagatgttc gcggtcgcgc tgaacgtgca ggtcctggga tcgtcgacga
     2401 cgccgtcggt caccccgtcg gcggcgtcgc aggcggccac ggcggaggcg gtggcctggt
     2461 tgagcttggc cgccgagatc ggcccgccgg ctagctcctt cattgcgatc tctccccaca
     2521 tctgcgcggt ctggaaccgc gtccagtaga tcgccggcgc gctggccagg atcccgtcga
     2581 gctcgccggc cagctcctgc gcgagcaggt agccctggcg ccctcccgtc gagcagccgt
     2641 tccagtaatt gaacgcgggc ttcatgccat agtaggcgtc tgccacccgc ttcgagagca
     2701 gcacctgctc cttgatcgcg ttgcggatga agtcctcgat gaactgcgtg ttgtacgtcc
     2761 cgtcggcatt gacccccggc tcgcagttgc cgcccgcgtg tcccaggtcg gtccccgagc
     2821 ctgcgtagcc ggtgttcacc gcgggggtca cgatcaggtt gccggcgcag ccaccgccgc
     2881 cgaggccctg cgtcctgccg ttccacgcgc cctgcacacc gccggtgccg ccgtccgccg
     2941 cgtcgagcgg cagcccgacg cggatgttga tgttctgcgc ggggttggtg ccgaacagga
     3001 tgtcgacctg acagtagctc gcattcgcgc cgctcactgc gatgatctgg ctggtcaccg
     3061 acttgatcac cggattgccg gcgaggccat gccgcggatc ggtggcaagc tccccgcagt
     3121 gcacggcggc cgcggggtgc gatcgaacgt cggccagcgc tcgtcgaggg ctcagcgccg
     3181 cgacgtacag gagcgcagga acaagtagga cgtagcttcg cctgtgcatg ggtccactcc
     3241 ttggtcggtg gtcagtccgc ggccacgagt ctcggagtcg ctgcgtggcc ggacaagatc
     3301 ggatctggaa tcagtgattc cacaaatccg tctgcgcgcg tcggcacaca gcccgctcga
     3361 cttgcccgcc ggccgggcgt agcgtgcaca gccatgcgcc gccttcgctt gctccccgcc
     3421 gtcgccgtgt ccgccggtct cgctggtctc gctgcgctcg ccggcctcgc cggtccggcc
     3481 ctcgcgcagg ccccggcgcc gctgccgccc gccgccgccc agaagctcgc ccagggcacg
     3541 ttccgcgagt acctcgagct cctcgcgctg cccaacgacg ccacccgcgg cgccgacatc
     3601 cagcgcaacg tcgagttcct cgagcgcgcc ttccgcaagc gcggcttcac cacccgccgg
     3661 ctcgacaacg gcgagcggcc gctgctgtac gccgagtggc cgacccagcc cgccggggcc
     3721 aagaccatcc tctactacat gcacctcgac ggccagcccg tcgtcgaccg cgagtggtcg
     3781 cagcccagcc cgtggcagcc ggtcgtcaag cagaagaacc gcgcgggcgg ctgggacgtc
     3841 gtgcccagcg acacgctgtt cgcgccggcg ctcgaccccg agctccgcgt gttcgcgcgg
     3901 tccgcctccg acgacaaggc gccgatcatg atgttcctcg ccgcgttcga cggcctccgc
     3961 caggccggcg ccgcgcccgc caaccacgtc aaggtcctga tcgacagcga ggaggagaag
     4021 ggctcgccgt cgatccgcgc ggtggtcacg gccaacaagg acctgctccg ctgcgacgcg
     4081 ctgatcatcc aggacggccc gcgccacgcc agcgagcggc cgaccctggt gttcggcaac
     4141 cgcggcgccg cccacgtcgt gctcactgtc tacggcccca ggcagccgct gcacagcggc
     4201 cacttcggca actacgtgcc caacccggcg atccggctcg cgcgcctgct cgcgtcgatg
     4261 aaggacgacg acggacgggt caccgtcgcc ggctactacg atcgcgtcca gctcaccgcc
     4321 gccgagaagc aggtcctcgc cgagaccggc gacgacgagc cggcgatccg ccgccgcgtc
     4381 ggcatccgct ccgccgagcg cgtcggggcc agcctccagg aggcgctgca gttcccgtcg
     4441 ctcaacgtcc gcggcatggc cgccgcctcg gtcggcgaca aggcctccaa catcgtcccg
     4501 caccaggcga ccgccgatat cgacatccgg accaccccgg agaccccgcc cgactacctg
     4561 ttcggcctgc tgcagaagca cgtcgaggcc cagggctatc acctcgtccc cggcccgccg
     4621 accgacgagg atcgcgccag ctacgacaag ctcgccacgc tcgccatgga tattggcagc
     4681 cgcgcggtcc gcaccccgct cgacgcgccg ctcggcacgt gggcctacgc agcgctgcag
     4741 caagccagcc agcgcggcgc gccggtccgg atccggatga tgggcggcag cgtcccgacc
     4801 gacagtctgg tcgaggtgct cggcgccccg ttcgtcatca tcccgctggt caacggcgac
     4861 aacaaccagc acacgttcga cgagaacatg cgcgtcggcc actacgtcga gggcatccgc
     4921 gcgatgctcg ccatgctgcg gcggaagttc tagcccgcgt atcgtcgcgc gccgccgcag
     4981 ctgggcctgc atgacacgct cgccaggcga ggagtctcca cgagatgcgc cggtcctggt
     5041 ttgtgccagc agtggaccga acgagctgcc gagcggccga cgagagacca cgatgttcgg
     5101 cgcatgcggc tgcggccagg gtcacgggag accatgtgga cgggcttgtg actggcccgc
     5161 cgaagtgggc taggtcgacc aggacggccc actcacaatc catcgccctg cgcggatgct
     5221 ccgtttcgga atcatgttga ttttgatggg atgttcggag aggcgcactc gacatctcgg
     5281 tgagctttca tcacagccgc cgtggtccca gctgggactg ccgtgagtcg cggcgctccc
     5341 gcgtgtccga tccatgccct gatctgtaaa tcattgaaat tccaggcggg atcgcaacgc
     5401 ccaagccagg caagtatgtc gcttcgcgca tgaggtctcc aaccgggctc aagtccggct
     5461 gggatcacga cttcgaggcc tggccaaccg gttcatgaat gcgagatcac ggaagcccct
     5521 gccggtcaca tccggagatc ggatcaaaat caaattcctg cgagacggag catttgcgcg
     5581 gggcgatgga ttgtgagtgg gccgtcctgg ttgaccaggt ccacttcagc gggccagtca
     5641 caagcccgtt cacatggtct cccgtgattc tggccgcggc cgcatgcgcc gatcatcgtg
     5701 gtctctcgtc cgctcgttcg atccactggc gacaaccatg acccgcgcac ctcgtggaga
     5761 gtcctcgcgc gccgggcgtc gcacgggccc tgacctgtcg attcgagctg gactcggacg
     5821 agcttgatat ttaccaatag tgcaatattc tcgtaataaa tatatgccgt ttcaattgca
     5881 tgattaccag tcaggaatta tcaagagacc atggcagcgg gaagtccagg tcggaagcga
     5941 aagctccagc aaccccagct ggtgctgttc aagaagcgcg gcggcaagcg gcgcggcgca
     6001 ggtcgtccgc ccaagggcgc acgagccggc tcgccgcaca agccgcggcc gtacctccac
     6061 gcccgctacc cggtccatgt cacgctgcga gtcgtcggcg cggtgggcaa cctgcgccgt
     6121 cgctgcgtct atcaggcgat ccgcgaggcc acgctgacca ccgcgcggcg cgaggacttc
     6181 cgcatcgttc atctcagcat ccagcgcacg cacgtccact tgctcgtcga ggccaacgac
     6241 aaggctgcac tggcgagggg catgcagggc ttccagatct cggccgccaa gcacctcaac
     6301 gcagcgatca gcaagggcaa gccgggcgca cgccgccgag gcgtggtgtt ccccgaccgc
     6361 taccacgccg agatcatcac gtcaccgcgt caagtgcggc actccctggc gtactgcttg
     6421 aacaactggc gtcgccatca ggaggaccgc cgggctccga tgtctggctg gacgattgac
     6481 tggttctcca ccgcggtgat gtttccgggc tgggtggagt acggcgacga gccattcctg
     6541 tggcgcggtc ctgcgaccta cgacccgatg gtggtgtacc agcccagaac ctggctgctg
     6601 agagagggtt ggaagaaggc cggagcggtc tcgtgccacg aggtccccgc tgcaggaagg
     6661 tgaagcgccc ggcgcatcac gtacgccttg tccccgtggc taccgcgcct cgatcggctt
     6721 ggtcgggcgg gcgaggagga acgcgcgcgt gaatgcggcc gcggtctcgc acgcgtacgc
     6781 ggtccagtcg gggtggcgct cgtagccgac cacgtcgctg acgccgcgga tcgcgaggaa
     6841 cgggacgcgg tcgtgggtgg ccttgtagac gccggcggac tccatctcga cggcgatgac
     6901 ctggcgggcg gactcctgcc agcgattgag gagggcggtg tccttgatga gccgatcgct
     6961 cgagctgatg gccccggtca ccgcgagcgg cgcccgcggt gcgccgccga agtggcgctc
     7021 gaggtccgct tgtagcttgg ccttgcttgc cttggtgccg tagaacttgc ttggcgatag
     7081 atcgaccaga gggcggggct tgccgatcgc ggtgtcggtg ttccagccat cgagctcccc
     7141 gtcggcgatc atcgccttga tgtcggcggc gagccccgcg gcttccgggt cgagcgggcc
     7201 gcctccgtgg gcgtacgtgc gctcgccact gtccttcacg gcctccaccg acatgtcgac
     7261 gatacggctc gagatgacga cgtcgccgag ggagaactcg gacgacggtg cgccgccggc
     7321 gatcccgacc accaggacaa accccgggga caggtcgtca agcaggtctc gcgccgccat
     7381 gagcgagccg gtgttgcctt gctccagcga gcggagcacg gcgatcgtgt aggcaccgcc
     7441 gcccgggagc ttcagccggc gaatgcggta gcggcgcttc tgaacctcga ccgcgtgctt
     7501 gtcgaaccgg cgcaggacgg cctcgttttc gtcctcgcgg atcgtgacga tgccaaagtc
     7561 gatcttcccg tggtaggggc tgcgtccgat cgcctcggag gtcgcctggg atacgcgagc
     7621 tcgacggtgc gaagagtcgt cacgcacgcc ttcgagtagc tcctgcaccg agtatttgcg
     7681 actggcacct tcgggacgcc cggtgtagtc ggggccctcg tcgcgttcca gctggaccag
     7741 gtgcgcgtag ccga
//
LOCUS       DASVOR010001665         2327 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_338189,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001665 DASVOR010000000
VERSION     DASVOR010001665.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338189"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF516_31175"
     CDS             complement(<1..375)
                     /locus_tag="VF516_31175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8112252.1"
                     /translation="MVTADLLSRARAGDGEAFRELAEPHRRELQVHCYRMLGSLQDAE
                     DAVQETLLAAWQGIGGFTEERASLRTWLYKIATNRCLNARRAASRRPAREWDMSQFEA
                     PVPTPRDEAVWLQPFPDQLLEGA"
     gene            716..1048
                     /locus_tag="VF516_31180"
     CDS             716..1048
                     /locus_tag="VF516_31180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112253.1"
                     /translation="MLDPHLQLMLFHARQRELEDQAARDRLAASQRAERHALGRLLTA
                     AGTALLALGTRLHGAPAPEHVSDRRLVEANGHSAQLDAVCQPGALVNVRIPRRSPCTG
                     KQPPAGSE"
     gene            1066..>2327
                     /locus_tag="VF516_31185"
     CDS             1066..>2327
                     /locus_tag="VF516_31185"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8112254.1"
                     /translation="MFAGWRTGLPGPVLVLQAATAVNSFGTGLILPFEIIYLHQARGF
                     PTATAGLALAAIMGTAAVVTPLSGALLDRFRAKPILITGNLASALGYAAFAFVDRPWQ
                     AFVCAAVGGAGFGLASTANQVLTLTLVSAEHRASSIALRRVAGNFGLGSGATVAGFIV
                     AFAHDLRAFQALYLFDGVTFTVFALVVLVGIPNPRLANAASASDRGTGFRAVAHDRLF
                     LILLAANIVLVMTGGALFSNILAPFATAHTPVGPGEIGIVIFINTIFIVVAQIPATRV
                     VKRMRRTHALAATSALFAIGLLAVLLAPLTSSTLTATTVLAGVAIVIAIGECAQFIVL
                     GPIVADLAPPHLLGRYMALYGLTFTAGVALGPAVGGALLATSPDAVWWGGALAAALTG
                     AGLLRLGDRLPDPLLQAQCPAPQAVTVA"
BASE COUNT          322 a          879 c          735 g          391 t
ORIGIN      
        1 ggcgccctcg aggagttggt caggaaacgg ctgcagccag acggcctcgt cgcgcggcgt
       61 cggcacgggc gcttcgaact gcgacatgtc ccactccctg gccgggcgcc ggctcgccgc
      121 gcggcgcgcg ttgaggcacc gattggtggc gatcttgtac agccaggtgc gcagcgacgc
      181 gcgttcctca gtgaacccgc cgatgccctg ccaggcggcc agcagtgtct cctggacggc
      241 gtcctcggcg tcctgaaggg atccgagcat ccggtagcag tgcacctgca gctcccgccg
      301 gtgcggctcg gccagctccc ggaacgcctc gccatccccg gcccgcgccc gggagagaag
      361 atcggctgtc accatcctgc gcaccttcct tccctcacca cgtcgtccct ccgccccagt
      421 atatacaccg ccccagtata tagccccagt atatacaccg cccacggcgc gaactgggcg
      481 gctcgcgggc gcccagtttc ccagtgccgc ggtgtatgca ctaccgactg ccggacgatc
      541 cgcccaccgg atcgtgccgc tccagctgga accgaccctg gaaggtaacc catggaattg
      601 tccgcaccgg ccgggcgcgg ggcgttcgcc gttcgcgatc gccgccgcgg ctggaccctc
      661 tgcgccctcg cgcggctccg acgcccccat ctccgaaagg acaggaacac acaccatgct
      721 cgatccacat cttcagctca tgctgtttca tgcgcgccag cgggagctgg aagatcaggc
      781 ggcacgcgac cggctcgcgg cgtcgcagcg cgcagagcgc cacgcgcttg ggcgcctgct
      841 gacagcggcg ggcacggccc tgctcgcgct gggcacacga ctacacggcg cgcccgcgcc
      901 cgagcacgtc tccgaccggc gcctggtaga ggcgaacggc cacagcgcgc agttggacgc
      961 cgtatgtcag ccgggggcgc tggtgaacgt gcgcatcccg aggaggagcc catgtacggg
     1021 aaagcagcca cccgcaggca gcgagtgaga agaacgctcg ctcgtgtgtt cgccggctgg
     1081 cggacgggcc tgccggggcc tgtcctcgtt cttcaggccg cgaccgcggt caactccttc
     1141 ggcactggac tgatccttcc cttcgagatc atctacctgc accaggcccg cggcttccca
     1201 acggcgaccg cggggctggc cctggcggcg atcatgggca cggcggcggt cgtcacgccc
     1261 ctgtcgggtg cgctgctcga tcgtttccgt gcgaagccga tcctgatcac cggcaatctc
     1321 gcgagcgcac tcggctacgc ggccttcgca ttcgtcgacc gtccctggca agccttcgtc
     1381 tgcgcggccg tcggcggcgc tgggttcgga ctggcgagca ccgcgaacca ggttctgacc
     1441 ctgaccctgg tctcagcgga gcaccgcgcc tcctcgatcg cgctccgccg ggtcgcaggc
     1501 aacttcggtc tcggcagcgg cgcgacggtt gcgggcttca tcgtcgcttt tgcccatgac
     1561 ctccgcgcgt tccaggcgct ctacctcttc gacggcgtca ccttcactgt cttcgcgctg
     1621 gttgtgctcg tggggatccc gaatcctcgc ctcgccaatg ccgcgtcggc gagcgacagg
     1681 ggaacgggct ttcgagcagt tgctcacgat cggctcttcc tcatcctgct cgccgccaac
     1741 atcgtgctcg tgatgaccgg cggcgcgctc ttttccaaca tcctcgcgcc gtttgcgacg
     1801 gcgcacacgc ctgtcgggcc gggcgagatc ggcatcgtca tcttcatcaa caccatcttc
     1861 atcgtcgtcg cgcagattcc agccacacgg gtcgtcaagc ggatgcgccg cacgcacgct
     1921 ctcgccgcga ccagcgcgct ctttgcaatc ggcctgctcg cggtcctgct tgcaccgctc
     1981 acgagctcga cgctcaccgc taccaccgtc ttggccgggg tcgcgatcgt gatcgcgatc
     2041 ggtgagtgcg cgcagttcat cgtgctcggc ccaatcgtcg ccgacctcgc accgccgcat
     2101 ctgctcggcc gctacatggc gctctacggc ctcaccttca cggcaggcgt ggccctcggc
     2161 cccgcggtcg gcggcgcgct gctcgcaacc tcccccgacg ccgtctggtg gggcggcgcc
     2221 ttggcggcag cactgaccgg agccggcttg ctgcgactcg gcgaccggct acccgacccg
     2281 ctcctgcagg cgcaatgccc tgcgccgcaa gccgtcactg tggccga
//
LOCUS       DASVOR010001666         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_573102,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001666 DASVOR010000000
VERSION     DASVOR010001666.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573102"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(537..956)
                     /locus_tag="VF516_31190"
     CDS             complement(537..956)
                     /locus_tag="VF516_31190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112255.1"
                     /translation="MKLTRSLMFVAVFAGALAALAPVARAGTGCPDDPERCVERGAAT
                     PHRVVSIANNTRTVTTPAPAAVNTPAAARKPSVTTSKSVAKKPAAKPTRPAPSVSAPT
                     PATPGMGMLLKMSGGNGGDVSWSPSRPVDTTGASWIL"
     gene            complement(1304..>1802)
                     /locus_tag="VF516_31195"
     CDS             complement(1304..>1802)
                     /locus_tag="VF516_31195"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX8112256.1"
                     /translation="GAINPELMPAELMLATGHLNFISKRNLLTTAELRERRLGRKVGD
                     PYSPRVRAALLDAALEAGVKLHQGVLMGGHGPTYETASEVRMAARLGADVACMSTVHE
                     VTLAAQLGCETASLSCITNRATGLSATPLTHHEVTEVADQVAVRLRAVLEKFLDAEAA
                     KPARG"
BASE COUNT          247 a          598 c          688 g          269 t
ORIGIN      
        1 ggtcgacgag ttcggccttc ttggccgggg tcgcgggggt cgccggagtc gcgggtgtcg
       61 ccacagtggg cgcggtggcg gcatgcgtgg ccggcttcgg tgcggcgtat gcgatcgaca
      121 cggacagcag cgcggcgagg acgacctgca acgggagcga gcgcttcatc cacgacctcc
      181 aggtgtgcga ctgctgcctg cgatgcgcgt cggggcggcc agcgcgcaac atgatggtgc
      241 gcgtccgcgc gcccgtgtgc aatcccaaga gtcgtgcggc gaagattgtt ggcggagtct
      301 tatcagcccc attcggcccc cgtccgccac cgagcgacaa cgaacgatca tggcccgagg
      361 acgcgccaac gagcttcgcg gcacgactcg agtctcagcg gggtcatgag agtgagagtc
      421 ggaagtcgtg gtcgccggcg gcggcagcga tcttccgcgc accacccgca cggccgcaaa
      481 gcaacacgcc gcgccacccg aaggtgacgc ggcgtgcggt acgagccgcg gtacttctac
      541 aggatccagc tggcgccggt cgtatcgacg gggcggctgg gggaccagga cacgtcaccg
      601 ccgttgccgc ccgacatctt gagcagcatg cccatgcccg gcgtggcggg cgtcggcgcc
      661 gaaacgctgg gcgcgggacg cgtgggcttg gcggccggct tcttcgccac cgacttggag
      721 gtggtgaccg agggcttgcg cgccgccgcg ggcgtgttca ccgctgcggg agccggcgtc
      781 gtcacggtgc gcgtgttgtt cgcgatcgag accacgcggt gcggggtcgc cgcgccgcgt
      841 tcgacgcagc gttccggatc gtccgggcag ccggtgccgg cgcgcgcgac gggcgcgaga
      901 gcggcgagtg ctccggcgaa tacggcaacg aacatgagtg agcgagtcag cttcacgaac
      961 gacctccgtg aggtgctccc ggtactcgag tgccgcgact atacaacgga agtcccagcc
     1021 gggtatgggt ttttgtgacc ggggtaccta tgtgacgggc gcgggggcca aagggttgca
     1081 agctgtcatg gagatgtcac atgcggcccc gaggagcgtc gggccactcg gcggcgcgcg
     1141 ggcctggttg gcgaggttcc tgccgcgccg tcagccacgg cgggtcgccg gcactcggcc
     1201 tcgctcgcga gcgcttcccg gtgggggagc gccgtcgatc agccgcgagc ggagttcacg
     1261 tgcttcgaag cggcgggctc gcgatcgcgc gcgcgccggg cgttcagccg cgagcgggct
     1321 tcgcggcctc ggcgtcgagg aacttctcca gcaccgcgcg cagtcgcacc gcgacctggt
     1381 cggccacctc ggtgacttcg tggtgcgtga gcggcgtggc cgagagcccg gtggcgcgat
     1441 tggtgatgca cgacaggctc gccgtctcgc agcccagctg ggcggcgagc gtgacctcgt
     1501 gcaccgtgct catgcacgcc acgtcggcgc ccaggcgcgc ggccatgcgc acctcggagg
     1561 ccgtctcgta ggtcggcccg tggccgccca tgagcacgcc ctggtgcagc ttcacgccgg
     1621 cctcgagcgc ggcgtcgagc agcgcggcgc gcacccgcgg cgagtacgga tcgccgacct
     1681 tgcggcccag cctgcgctcg cgcagctcgg cggtcgtgag caggttgcgc ttgctgatga
     1741 agttgagatg cccggtggcc agcatgagct cggccggcat gagctcgggg ttgatcgcgc
     1801 cc
//
LOCUS       DASVOR010001667         2218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_484816,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001667 DASVOR010000000
VERSION     DASVOR010001667.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484816"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VF516_31200"
     CDS             complement(<1..641)
                     /locus_tag="VF516_31200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8112257.1"
                     /translation="MTVRKSGDRGHADHGWLDSRHTFSFADYHDPAHMGFRALRVINE
                     DRVAPGQGFGRHSHRDMEILSYVLDGGLEHKDSMGSGAVIRPGDVQRMSAGTGVIHSE
                     YNASRQAPVHFLQIWIMPDRRGLPPSYEQKTFSEADKAGKLRLVASPDGADGSVTIHA
                     SARLYAGVFGAGQSAEHPLDPGRHAWVHMVRGSARVNGTELGAGDAVALSEERT"
     gene            739..1692
                     /locus_tag="VF516_31205"
     CDS             739..1692
                     /locus_tag="VF516_31205"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8112258.1"
                     /translation="MLETFTLDQLRALIAVSEEGSFSAAARKLHRVQSAVSTAMANLE
                     TQLGVALWDRSTKVARLTEQGQAVVAAARKVCAEVDGLRQLAAGMVAGLEASVSLCVD
                     MLFPVPALVALCKQFRDEFPSVDLRVDTQTMTGVSARVIEGRATIGVAAPMGVAAGLE
                     RKLLTAIQMVPVAAPGHPLASVRGAISTGRLRDEIQLVLSELQDEGHPDQAVLSSRTW
                     RVRDLHTKHALLRAGLGWGNLPEHLVRDDLRDRRLVKLRVEAWGEDEHTLYLSAVYRG
                     DATFGPAHRWLLSQLEHLCLRDAERAARPAARRPRQRARTG"
     gene            complement(1689..>2218)
                     /locus_tag="VF516_31210"
     CDS             complement(1689..>2218)
                     /locus_tag="VF516_31210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112259.1"
                     /translation="TRQPCTSTFGSAMTATFGRLDGFLVSIVNPSTTTSCRGDDNHIH
                     LQVQVNGGIEDIAVNVASTSGNPDVFFRTVGAALRGGAWTEGWHPGQTLDYPSNLGVS
                     SASFTEETLTQLTASVDNLIANANHVSIFATGFDPTGGHLVHREGSSHDGAIVINPTT
                     SNPTYLLFHFTTQTF"
BASE COUNT          290 a          769 c          809 g          350 t
ORIGIN      
        1 gtccgttcct cggacagggc gaccgcgtcg ccggcgccga gctccgtgcc gttgacccgc
       61 gcgctgccgc gcaccatgtg cacccaggcg tggcggccgg gatccagcgg gtgctcggcg
      121 gactggccgg cgccgaacac gcccgcgtac agccgcgcgc tggcgtggat cgtgacgctg
      181 ccgtcggccc cgtccggcga tgccaccagc cggagcttgc cggccttgtc ggcctccgag
      241 aatgtcttct gctcgtagct cggcggcagc ccgcggcggt cgggcatgat ccagatctgg
      301 aggaagtgga ccggcgcctg gcgcgacgcg ttgtactcgc tgtggatgac tccggtgccg
      361 gccgacatcc gctggacgtc gccggggcgg atcaccgcgc ccgagcccat cgagtccttg
      421 tgctccaggc cgccgtccag cacgtacgag aggatctcca tgtcgcgatg cgagtggcgg
      481 ccgaagccct ggccgggagc gacccgatcc tcgttgatca cgcgcagcgc gcggaacccc
      541 atgtgcgcgg ggtcgtggta gtcggcgaac gagaaggtgt gccggctgtc gagccagccg
      601 tggtcggcgt ggccgcggtc accggacttg cgaacggtca tcatgttgtc ctctggacgg
      661 cctagtttgc gcattccggg ccatcaaagg aactggcaat cgattgatgt ctttcatcgc
      721 tccgtgagat ggtgcacgat gctcgaaacc ttcacgctcg accagctccg cgcgctgatc
      781 gccgtcagcg aggagggcag cttctcggcc gcggcgcgca agctccaccg cgtccagtcc
      841 gcggtctcga ccgcgatggc caacctcgag acccagctcg gcgtggcgct gtgggatcgc
      901 tcgaccaagg tcgcccggct caccgagcag ggccaggcgg tggtcgccgc cgcccgcaag
      961 gtgtgcgccg aggtcgacgg cctgcgccag ctcgccgccg gcatggtcgc cggcctcgag
     1021 gcctcggtgt cgctgtgcgt cgacatgctg ttcccggtcc cggcgctggt cgcgctgtgc
     1081 aagcagttcc gcgacgagtt cccgtcggtc gacctccggg tcgataccca gacgatgacc
     1141 ggcgtgtcgg cgcgcgtgat cgagggccgc gcgacgatcg gcgtcgcggc gccgatgggc
     1201 gtcgcggccg gcctggagcg caagctgttg accgcgatcc agatggtccc ggtcgccgcg
     1261 cccggccacc cgctcgcttc cgtgcgcggc gcgatctcga ccgggcggct gcgcgacgag
     1321 atccagctcg tgctgtccga gctccaggac gagggtcacc ccgatcaggc ggtgctgtcc
     1381 tcgcggacct ggcgggtgcg cgacctccac accaagcacg cgctgctgcg cgccggcctg
     1441 ggctggggca acttacccga gcacctggtc cgcgacgacc tgcgcgatcg ccggctggtc
     1501 aagctccgcg tcgaggcctg gggcgaggac gagcacacgc tgtacctgtc ggcggtgtac
     1561 cgcggcgacg ccacgttcgg tccggcgcac cgctggctgc tgtcgcagct cgagcacctg
     1621 tgcctgcgcg acgccgagcg cgcggcacgc ccggccgcgc ggcggccgcg ccagcgggcc
     1681 aggacgggct agaacgtctg cgtcgtgaag tggaacagca ggtaggtcgg gttcgacgtc
     1741 gtcgggttga tcacgatcgc gccgtcgtgg ctcgacccct cccggtggac gaggtggccg
     1801 ccggtcgggt cgaacccggt cgcgaagatc gagacgtggt tggcgttcgc gatcaggttg
     1861 tcgaccgacg cggtcagctg agtcagggtc tcctcggtga agctcgccga gctcacgccg
     1921 aggttgctcg ggtaatcgag cgtctggccc gggtgccagc cctcggtcca cgcgccgccg
     1981 cgcagcgccg cgccgacggt acggaagaag acgtccgggt tgccgctggt cgacgcgacg
     2041 ttgaccgcga tgtcctcgat cccgccgttg acctggacct ggaggtggat gtggttgtcg
     2101 tcgccgcggc acgacgtcgt cgtgctgggg ttcacgatcg acaccaggaa gccgtcgagc
     2161 cggccgaacg tcgcggtcat cgccgagccg aacgtgctgg tgcacggctg gcgggtcg
//
LOCUS       DASVOR010001668         2427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_18228,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001668 DASVOR010000000
VERSION     DASVOR010001668.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18228"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1663
                     /locus_tag="VF516_31215"
     CDS             <1..1663
                     /locus_tag="VF516_31215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112260.1"
                     /translation="PASQPPAAPAPAAAPDTGYVGREITPLEIDDCKSTELSSGEVIR
                     QGSEHYDRGETLYLQGDYEGAVREWVYSYCLVPAFYTILKDIGQAYERNLDYEKAIGY
                     LERYVRAVPPPGTPRCSVCPADPQQDKATVARRVEVLKKLKAKIYVESQPPGAQITIA
                     NDAGVAARAHSGETIEVLGGHYDMVTELDGYQPHHQAIDVRVGKPYTYFVPLVALHGQ
                     LSMQVLPPDARIFLGDRLVGIGHIDLALPANTYVVTSEAPDRITDRRRVEVLADQVKK
                     VQVELTAQPQFGRRQLIAFATVAGAGATGSMLYAFHNTGLTGLGAVGGGATGLVGSVF
                     LLPDVPLGTSNLTVTSTTGGAILGAGASLLFTRRPEVIYPVVGLGAVLGGTAGYLVGD
                     AIRINVGDAALINSGVVWGSVSGALFAASFDPGHAVGGGLLLSGLGMGTVGGLLLQSN
                     FSISRTHAVLIDVGGMVGIIGGLALESLVYPTQTVQAPSPVIDARAQEHLADYALGGM
                     AIGLLTAGILTRNLDDPKIPVLPAITQATAGDGRAATVYGVAGRW"
     gene            1741..>2427
                     /locus_tag="VF516_31220"
     CDS             1741..>2427
                     /locus_tag="VF516_31220"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8112261.1"
                     /translation="METHSSACPLDCPDLCGLTVTVDQGRVVEVDGDRRGPLTDGFIC
                     GKVRTIADHMYGEDRLTTPLVRTGPKGSGRWSPVSWDDALERIAGRIAEIRARAGGEA
                     ILPYNYGGSNGWLTEGALASRFFRRLGASNLERTLCATATTAATRGLYGVMPGVALED
                     YVHARLIVLWGVNPSATGIHLVPVIERARAAGVRVELAVEGDVHPLAQSVDLSAFRIL
                     QEALTNVVKHA"
BASE COUNT          338 a          899 c          868 g          322 t
ORIGIN      
        1 cccggcatcg cagccgcccg cggccccggc gcccgcggcc gcgcccgaca ccggctacgt
       61 cggccgcgag atcacgccgc tcgagatcga cgactgcaag tcgaccgagc tgagctccgg
      121 cgaggtgatc cgccagggct cggagcacta cgatcgcggc gagacgctgt acctccaggg
      181 cgactacgag ggcgcggtcc gcgagtgggt ctacagctac tgcctggtgc cggcgttcta
      241 cacgatcctc aaggacatcg gtcaggccta cgagcgcaac ctcgactacg agaaggcgat
      301 cggctacctc gagcgctacg tccgcgccgt gccgccgccg ggcaccccgc ggtgcagcgt
      361 gtgcccggcc gacccgcagc aggacaaggc cacggtcgcc cgccgggtcg aggtgctgaa
      421 gaagctcaag gccaagatct acgtcgagtc gcagccgccc ggcgcgcaga tcacgatcgc
      481 caacgacgcc ggggtcgcgg cgcgcgcgca ctcgggcgag accatcgagg tgctcggcgg
      541 ccactacgac atggtcaccg agctcgacgg ctaccagccg caccaccagg cgatcgacgt
      601 ccgggtcggc aagccgtaca cgtacttcgt cccgctggtc gcgctgcacg gccagctgtc
      661 gatgcaggtg ctgccgcccg acgcccggat cttcctcggc gatcgcctgg tcggcatcgg
      721 ccacatcgac ctcgcgctcc ccgcgaacac ctacgtggtg accagcgagg cgccggaccg
      781 gatcaccgac cggcggcggg tcgaggtgct cgccgaccag gtcaagaagg tccaggtcga
      841 gctcaccgca cagccgcagt tcggccgccg ccagctgatc gcgttcgcga ccgtcgccgg
      901 cgcgggcgcg accggctcga tgctctacgc gttccacaac accgggctga ccgggctcgg
      961 cgcggtcggc ggcggcgcga ccgggctggt cggcagcgtg ttcctgctcc ccgacgtgcc
     1021 gctcggcacc agcaacctga ccgtgacgtc gaccaccggc ggcgcgatcc tcggcgcggg
     1081 agcgagcctg ctgttcacca ggcggcccga ggtgatctac ccggtggtcg gcctcggcgc
     1141 ggtgctcggc ggcaccgcgg gctacctggt gggcgacgcg atccggatca acgtcggcga
     1201 cgcggcgctg atcaacagcg gcgtggtgtg gggcagcgtc tcgggcgcgc tgttcgcggc
     1261 gtcgttcgac cccgggcacg cggtcggcgg cggcctgctg ctcagcggcc tgggcatggg
     1321 cacggtcggc ggcctgctgc tgcagagcaa cttctcgatc agccgcaccc acgccgtgct
     1381 gatcgacgtc ggcggcatgg tcgggatcat cggcggcctg gcgctcgaga gcctggtgta
     1441 cccgacccag accgtgcagg cgccgtcccc ggtgatcgac gcccgcgccc aggagcacct
     1501 ggcggactac gcgctcggcg gcatggcgat cggcctgctc accgccggca tcctgacccg
     1561 caacctcgac gatccgaaga tcccggtgtt gccggcgatc acccaggcca ccgccggcga
     1621 cgggcgcgcc gcgacggtgt acggcgtcgc cgggcgctgg taactcggct ccggcgttcg
     1681 gtccgggccg gcgagatctg taccggcgca ccgccgccgg acgcgcgtat agtgcgcacg
     1741 atggagaccc attcgtcggc gtgcccgctc gactgcccgg acctgtgcgg cctgaccgtc
     1801 accgtcgatc agggccgcgt ggtcgaggtc gacggcgacc gccgcggccc gctcaccgac
     1861 ggcttcatct gcggcaaggt ccgcacgatc gccgatcaca tgtacggcga ggaccggctg
     1921 accacgccgc tcgtgcgcac cgggcccaag ggcagcgggc ggtggtcgcc ggtatcgtgg
     1981 gacgatgcgc tcgagcggat cgccggccgg atcgccgaga tccgggcgcg cgccggcggc
     2041 gaggcgatcc tgccctacaa ctacggcggc tcgaacggct ggctgaccga gggcgcgctg
     2101 gccagccggt tcttccgccg gctcggcgcc tcgaacctcg agcgcacgct gtgcgcgacg
     2161 gcgaccaccg cggcgacccg cgggctgtac ggcgtgatgc cgggggtcgc gctcgaggac
     2221 tacgtccacg cccggctgat cgtgctgtgg ggcgtcaacc cgtcggcgac cggcatccac
     2281 ctcgtgccgg tgatcgagcg cgcgcgcgcg gccggcgtgc gcgtggagct cgccgtcgag
     2341 ggcgacgtgc atccactcgc ccagagcgtc gacctctcgg cgttccggat cctgcaggag
     2401 gcgctgacca acgtcgtcaa gcacgcc
//
LOCUS       DASVOR010001669         4168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_133851,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001669 DASVOR010000000
VERSION     DASVOR010001669.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133851"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1101)
                     /locus_tag="VF516_31225"
     CDS             complement(<1..1101)
                     /locus_tag="VF516_31225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX8112262.1"
                     /translation="MREAVIVASSRTPLAKSVRGSFNATRPDDLAAHAIRHAIGKVPG
                     LDPKDVEEVILGCGQPHGSAGHNIARVAALRAGLPVTVAGATVNRFCNSGLQAVVQAC
                     HMVMLEGVEAIVGGGVESITMMERDTSPNPWLVERIPALYMAMGDTAEVVAKRYGVTR
                     QAQDELSLASQQRIARAQREGWLKDELAPMQVRRNLLDKKTGAVTGQEDALVENDECN
                     RPDTTLEGLLKLPPVFDTKSGQGSVTAGNSSQLSDGASATLVMSADKARATGARPLLI
                     FRGYAVAGCEPDEMGIGPVFAIPKLLKKHGLKIDDIDVWELNEAFASQVIYCRDKLGL
                     PMDRLNPNGGSIAIGHPFGMTGSRLVGTIAYEM"
     gene            complement(1112..2485)
                     /locus_tag="VF516_31230"
     CDS             complement(1112..2485)
                     /locus_tag="VF516_31230"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEX8112263.1"
                     /translation="MADRTPSPVDPASGPASDPNAPEARALRHLAAAEQEALRVPGVP
                     DGTPVAAIQRAAVIGAGTMGGGIAMAYANAGIPVLLKDVDAAAVERGMATIRKNYEAT
                     VAKGKLSPEAYAQTLARITPTTTYDGFDTVDLVVEAVFEDMPLKKATFAELGRVTRPD
                     AILASNTSTLDIDEFGAASGRLPKVIGHHFFSPAHVMKLLEIVRGRETSPETLATSIA
                     LGRRLAKVPVVVGNCFGFVANRMLAYYRREALLLLEEGASVPQIDGVMTAFGMPVGPF
                     GMQDIAGLDVSYRIKQYLKALGKTRADGPDSDIPDQLHDMGRHGQKTGAGWYRYEGGR
                     NRVPDPLIDELAARAAASRGIQRRTIDDDEILARITTALANEGARILEEGFAYRAGDI
                     DVIYCYGFGFPRHRGGPMFHADTLGLPTVLARVLDYRRRFGDYWQPAHLLSRLAAEGR
                     GFHDSPQ"
     gene            2619..>4168
                     /locus_tag="VF516_31235"
     CDS             2619..>4168
                     /locus_tag="VF516_31235"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8112264.1"
                     /translation="MPSTPRATQVGRFLTSNVGPGDVFTREDLTADQKLFGQTAAEFM
                     RREVLPSAARLYARDWPFTRELLLKAGELDLLRLEIPEAYGGLGLDKASAAYVFEQIG
                     MNPSFGGSIGAHTGIGTMPLLYFGNAAQKAKYLPRLASGELIGAYALTEPGSGSDALA
                     AKTTAVLTPDGSHYVLSGRKMWITNGGFADLFTIFAKVDGTKFTGFLVERGMGVVSGP
                     DEHKLGLDGSSTTELILEDVRVPVENVLGTIGEGHKVAFNTLNLGRVKLGSRNLACGK
                     FALGNAARYARERKQFGKSIGEFGMIKHKLAEMSLRIFVGDAMVYRTLGDVDRALATV
                     DPADLPAVLSTIEQFAIECSINKVATSEILAYVVDEALQTYGGNGYSREFPAERPYRD
                     ARITRIYEGTNEINRLIIPTRILRAGIPAEVPAGGTGDRAVLAAAKRLTVAMLTSAAE
                     AFGERVAEHQEVLGHIANVVIDGYAIESALARSEKLAASGSDRTALAADMTAVFVADA
                     ADRIVHAA"
BASE COUNT          595 a         1402 c         1547 g          624 t
ORIGIN      
        1 catctcgtag gcgatggtgc cgaccaggcg cgagccggtc atgccgaacg ggtggccgat
       61 cgcgatcgag ccgccgttgg ggttgaggcg gtccatcggc aggccgagct tgtcgcggca
      121 gtagatgacc tgggacgcga aggcttcgtt gagctcccag acgtcgatgt cgtcgatctt
      181 gaggccgtgc ttcttgagca gcttggggat cgcgaacacc gggccgatgc ccatctcgtc
      241 gggctcgcag ccggcgacgg cgtagccgcg gaagatgagc agcggcctgg cgccggtggc
      301 cctggccttg tcggccgaca tcacgagggt ggcggaggcg ccgtcggaca gctgggagga
      361 gttgccggcg gtgacgctgc cctggccgct cttggtgtcg aacaccggcg gcagcttgag
      421 caggccctcg agcgtggtgt cggggcggtt gcactcgtcg ttctcgacca gcgcgtcctc
      481 ctggccggtg acggcgccgg tcttcttgtc gaggaggttg cggcggacct gcatcggggc
      541 gagctcgtcc ttgagccagc cctcgcgctg ggcgcgcgcg atgcgctgct ggctggcgag
      601 cgagagctcg tcctgggcct ggcgggtgac gccgtagcgc ttggcgacga cctcggcggt
      661 gtcgcccatc gccatgtaga gggccgggat gcgctcgacc agccacgggt tgggcgaggt
      721 gtcgcgctcc atcatcgtga tcgactcgac gccgccgccg acgatcgcct cgacgccctc
      781 gagcatgacc atgtggcagg cctggaccac cgcctggagg ccggagttgc agaaccggtt
      841 gacggtggcg ccggcgacgg tgaccggcag accggcgcgc agcgcggcga cgcgggcgat
      901 gttgtggccg gcggagccgt ggggctggcc gcagccgagg atgacctcct cgacgtcctt
      961 ggggtcgagg ccggggacct tgccgatcgc gtggcggatc gcgtgggcgg ccaggtcgtc
     1021 gggccgggtg gcgttgaacg agccgcggac gctcttggcg agcggggtgc gcgagctggc
     1081 gacgatgacg gcttctctca tggcggtgac ctcactgggg ggaatcgtgg aacccgcggc
     1141 cttcggcggc aagccgggag agcaggtggg ccggctgcca gtagtcgccg aatcgccggc
     1201 gatagtccag cacccgggcg agcacggtgg gcaggccgag ggtgtcggcg tggaacatcg
     1261 ggccgccgcg gtgacgcgga aacccgaagc cgtagcagta gatgacgtcg atgtcgccgg
     1321 cgcggtacgc gaagccctcc tcgaggatcc gcgcgccctc gttggcaagc gccgtggtga
     1381 tccgcgccag gatctcgtcg tcgtcgatgg tccggcgctg gatgccgcgg ctggcggcgg
     1441 cgcgggcggc gagctcgtcg atcagcggat cgggcacgcg gttgcggccg ccctcgtagc
     1501 ggtaccagcc ggcgccggtc ttctggccgt ggcggcccat gtcgtggagc tggtcgggga
     1561 tgtcggagtc ggggccgtcg gcgcgggtct tgccgagcgc cttgaggtac tgcttgatcc
     1621 ggtagctgac gtcgaggccg gcgatgtcct gcatgccgaa tggcccgacc ggcatgccga
     1681 acgcggtcat cacgccgtcg atctggggga ccgaggcgcc ctcctcgagc agcagcagcg
     1741 cctcgcggcg gtagtaggcg agcatccggt tggcgacgaa gccgaagcag ttgccgacca
     1801 cgaccgggac cttggcgagc cggcggccga gcgcgatcga ggtcgcgagg gtctcgggcg
     1861 aggtctcgcg gccgcgcacg atctcgagca gcttcatcac gtgggccggg ctgaagaagt
     1921 ggtggccgat caccttgggc agccggccgc tggccgcgcc gaactcgtcg atgtcgagcg
     1981 tcgaggtgtt ggacgcgagg atcgcgtcgg ggcgggtcac ccggccgagc tcggcgaacg
     2041 tggccttctt gagcggcatg tcctcgaaca ccgcctcgac gacgaggtcg acggtgtcga
     2101 acccgtcgta ggtcgtggtc ggggtgatcc gcgcgagggt ctgggcgtag gcctcggggc
     2161 tgagcttgcc cttggcgacc gtcgcctcgt agttcttgcg gatcgtcgcc atgccgcgct
     2221 cgaccgccgc ggcgtcgacg tccttgagca gcaccgggat gccggcgttg gcgtacgcca
     2281 tcgcgatccc gccgcccatg gtgccggcgc cgatcaccgc ggcgcgctgg atcgccgcga
     2341 ccggcgtgcc gtcggggacg cccgggacgc ggagcgcctc ctgctcggcg gccgccaggt
     2401 ggcggagcgc ccgggcctcg ggtgcgttcg ggtcggatgc gggaccggag gcgggatcga
     2461 caggggacgg cgtgcggtca gccatttagg tacctctcca gttcctaaat cggtccgggc
     2521 gcgatcacaa gcacctcacc gcggacaaga tgtcgtgctc gttgccggac atgttgataa
     2581 actgcgcggg tccttataac acgcacgacg gaagtcccat gccctccacc ccgcgcgcga
     2641 cccaggtggg tcgctttctt acctcgaacg tcggccccgg cgacgtgttc acgcgcgagg
     2701 acctcaccgc cgaccagaag ctgttcggcc agaccgcggc cgagttcatg cgccgcgagg
     2761 tgctgcccag cgccgcgcgg ctgtacgcgc gcgactggcc attcacccgc gagctgctgc
     2821 tcaaggccgg tgagctcgac ctgctccgcc tcgagatccc cgaggcctac ggcggcctcg
     2881 ggctggacaa ggcgagcgcc gcgtacgtgt tcgagcagat cggcatgaac ccgtcgttcg
     2941 gcggatcgat cggcgcgcac accgggatcg ggacgatgcc gctcttgtac ttcggcaacg
     3001 cggcgcagaa ggcgaagtac ctgccccggc tggcgagcgg cgagctgatc ggcgcctacg
     3061 cgctgaccga gccgggctcg ggctccgacg cgctcgccgc caagaccacg gcggtgctga
     3121 cgcccgacgg cagccactac gtgctgagcg gccgcaagat gtggatcacc aacggcgggt
     3181 tcgccgacct gttcacgatc ttcgccaagg tcgacggcac gaagttcacc ggcttcctgg
     3241 tcgagcgcgg catgggcgtg gtcagcgggc ccgacgagca caagctcggg ctcgacggct
     3301 cgtcgaccac cgagctgatc ctcgaggacg tcagggtccc ggtcgagaac gtgctcggca
     3361 cgatcggcga gggccacaag gtcgcgttca acacgctcaa cctcgggcgg gtcaagctcg
     3421 gctcgcgcaa cctggcgtgc ggcaagttcg cgctcggcaa cgcggcgcgc tacgcccgcg
     3481 agcgcaagca gttcggcaag tcgatcggcg agttcggcat gatcaagcac aagctcgccg
     3541 agatgtcgct ccggatcttc gtcggcgacg ccatggtcta ccgcacgctc ggcgacgtcg
     3601 accgcgcgct cgccacggtc gacccggccg acctgcccgc ggtgctgtcg acgatcgagc
     3661 agttcgcgat cgagtgctcg atcaacaagg tcgcgaccag cgagatcctg gcctacgtcg
     3721 tcgacgaggc gctccagacc tacggcggca acggctactc gcgcgagttc ccggccgagc
     3781 ggccgtaccg cgacgcgcgg atcacccgga tctacgaggg caccaacgag atcaaccggc
     3841 tgatcatccc gacccggatc ctgcgcgccg ggatcccggc cgaggtcccc gccggcggca
     3901 ccggcgaccg cgcggtgctc gccgccgcca agcggctcac cgtcgcgatg ctcaccagcg
     3961 ccgccgaggc gttcggcgag cgcgtcgccg agcaccagga ggtcctcggc cacatcgcca
     4021 acgtcgtgat cgacggctac gcgatcgaga gcgcgctggc gcgcagcgag aagctcgccg
     4081 cgtcgggctc ggaccgcacc gcgctcgccg ccgacatgac cgcggtgttc gtcgccgacg
     4141 ccgccgaccg gatcgtccac gccgccaa
//
LOCUS       DASVOR010001670         1715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_279261,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001670 DASVOR010000000
VERSION     DASVOR010001670.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279261"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF516_31240"
     CDS             complement(<1..326)
                     /locus_tag="VF516_31240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112265.1"
                     /translation="MIVPPTAHEEAAPIGGRLVTRFTAVLGLLVAVLVVVAGIRFVRG
                     IGAVTNLNDGYPWGLWIVYDDVIGTALGASGFVVAFATYVLNRGAYHPLVRPALVTAL
                     FGYVMA"
     gene            complement(323..1378)
                     /gene="hybA"
                     /locus_tag="VF516_31245"
     CDS             complement(323..1378)
                     /gene="hybA"
                     /locus_tag="VF516_31245"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF008134.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HEX8112266.1"
                     /translation="MLTRRKLLQGLAGGAGALAATSVASPAEARTKHAMPDQALGLLY
                     DGTLCIGCRACIPACKAANGMPPEQTELANGNYWDAPLDISGKTLTVIKAYRDGDGTH
                     KDQVKNGFAFTKVSCMHCVDPSCVSVCPVSAMTKDPTTGIVTYSLDACIGCRYCVAAC
                     PFGVPRFTYDTPFPRISKCQLCQHRLGEGRFAACAEVCPTGATLFGPVIDLKKEIARR
                     RSLAPGTRTTFPRGRIGGGDTCEGTAGTYVTHTYGETEIGGTQVLHLSGVPFGLLDKP
                     ALPDVAPASISESLQHTIYNHLIAPLGFLGVLAAAAWRHMRPPPQAVAAPEAAGAPED
                     PSTGEPPEGEPPEGEPR"
     gene            complement(1391..>1715)
                     /locus_tag="VF516_31250"
     CDS             complement(1391..>1715)
                     /locus_tag="VF516_31250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112267.1"
                     /translation="ANCPTIQFGDAGIGSWPVGCGHPCIGCTEQGVGFAKPIHELARL
                     LTVTPPTQFPRPGEDQGQGGTLATLGAVAAIGGLAIGAGARIAKNLGHLPDEPPPPAG
                     GEAQR"
BASE COUNT          234 a          595 c          641 g          245 t
ORIGIN      
        1 ctcgccatga cgtagccgaa cagcgcggtc accagcgcgg gccggaccag cggatggtag
       61 gcgccgcggt tcaggacgta ggtcgcgaac gcgacgacga agccgctggc gcccagggca
      121 gtgccgatga cgtcgtcgta gacgatccac aggccccacg ggtagccgtc gttgaggttg
      181 gtcacggcgc cgatgccccg gacgaaccgg atgccggcga cgacgacgag cacggccacg
      241 agcaggccga ggacggcggt gaaccgggtc acgagccggc caccgatcgg cgcggcctct
      301 tcatgtgcag tgggcggcac gatcatcgcg gctctccttc cggcggctca ccttccggcg
      361 gctcaccagt cgacgggtcc tccggtgcgc cggcggcctc cggggccgcc acggcctggg
      421 gtggcggccg catgtgccgc cacgccgccg ccgccagcac gccgaggaag ccgagcggcg
      481 cgatcaggtg gttgtagatc gtgtgctgca gcgactccga gatgctggcc ggggccacgt
      541 cggggagcgc cggcttgtcg agcagcccga acggcacgcc ggacagatgg agcacctggg
      601 tgcctccgat ctcggtctcg ccgtaggtgt gcgtgacgta ggttccggcc gtgccctcgc
      661 aggtatcgcc gccgccgatc cggccgcgcg ggaacgtcgt ccgggtcccc ggggcgagcg
      721 agcggcggcg cgcgatctcc ttcttgaggt cgatcaccgg gccgaacagc gtggcgccgg
      781 tcgggcagac ctcggcgcac gcggcgaacc gaccctcacc gagccggtgc tggcacagct
      841 ggcacttgga gatcctgggg aatggcgtgt cgtaggtgaa ccgcggcacg ccgaacggac
      901 aggctgccac gcaataccgg cagccgatgc aggcgtcgag gctgtacgtg acgatgccgg
      961 tcgtcgggtc cttggtcatc gccgacaccg ggcacaccga gacgcacgac gggtcgacgc
     1021 agtgcatgca cgagaccttg gtgaacgcga agccgttctt cacctggtcc ttgtgcgtgc
     1081 cgtcgccgtc ccggtacgcc ttgatgaccg tgagcgtctt gccggagatg tcgagcggcg
     1141 cgtcccagta gttgccgttc gcgagctcgg tctgctcggg tggcatcccg ttggccgcct
     1201 tgcaggccgg gatgcacgcc cggcagccga tgcacagcgt gccgtcgtac agcaggccga
     1261 gcgcctggtc gggcatcgcg tgcttcgtgc gggcctcggc cggcgaggcg accgaggtcg
     1321 cggcgagcgc gccggcgccg ccggccaacc cctggagcaa cttccttcgg gtcagcatgc
     1381 gtcctccgcg ctaccgctgg gcctcgccgc cggccggcgg tggcggctcg tcgggaaggt
     1441 ggccgaggtt cttcgcgatc cgcgcccctg cgccgatcgc gaggccgccg atcgcggcga
     1501 ccgcgccgag ggtggcgagc gtgccgccct ggccctggtc ctcgccggga cgcgggaact
     1561 gggtcggcgg cgtcaccgtg agcagcctgg ccagctcgtg gatcggcttg gcgaatccga
     1621 cgccctgctc ggtgcagccg atgcacggat ggccgcagcc gaccggccat gatccgatgc
     1681 ccgcgtcgcc gaactggatc gtcgggcagt tggcg
//
LOCUS       DASVOR010001671         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_396418,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001671 DASVOR010000000
VERSION     DASVOR010001671.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396418"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VF516_31255"
     CDS             complement(<1..1234)
                     /locus_tag="VF516_31255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112268.1"
                     /translation="MRQPVPFGKYLLLDRISVGGMAEVFKAKSYGVEGFEKIIAIKRI
                     LPTMGEDRDFIKMFIDEAKIAGQLAHANICQIFELGRIDGSHFIAMEYIWGKDLLQIQ
                     NRLRKIKQPMPIPMACFVIAKVLEGLDYAHRKRDPLGRPLEIVHRDCSPQNVLVSYEG
                     EVKVIDFGIAKATSRNSRTMAGVLKGKFGYMSPEQVRGLPLDRRSDLFALGTMLYECL
                     TSDRLFQGETDFSTLEKVRNVDVRPPREINPNIPPAVEAVIMKALAKDVEERYQWCSE
                     MLADLQAFLMSQDAVFTAKSLSSWLKDVFAGEIDRERQQLEQYKRVGRDGLIDGMPAA
                     EAKIDVVAGLGEAPPVEGDPTVLGGPNFDDIEQAAGASPEIGPAPGSPATRAASESEE
                     GEDFGEEAPTEIFGEIDHG"
     gene            complement(1427..>2192)
                     /locus_tag="VF516_31260"
     CDS             complement(1427..>2192)
                     /locus_tag="VF516_31260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112269.1"
                     /translation="VVALAAPGVGHAQPGRAPSDVLREGNTAALAGDWQRVADLVDPL
                     LREQLPVADLAEAHRLAGIAAFFAQRNASAEQHFVTYLRIEPDGRLDPALYPPDVVAF
                     FNDVASRHAAELRALRVRPERPSWLFTLIPPAGQYMNGDHTKAYVLGGVLGALLITNL
                     VTYSYLRQWCDHTQGSGGGQLTCTDGTGPGHDATQDARRLRPFNIASGIGFWVVYAFG
                     VYDGIRGYRRISREQALRPYVTVSTDGSVVGIHARF"
BASE COUNT          342 a          747 c          742 g          361 t
ORIGIN      
        1 cgccgtgatc gatctcgccg aagatctcgg tcggcgcctc ctcgccgaag tcctcgccct
       61 cctcgctctc cgaggccgcg cgggtcgccg gcgagccggg cgccggcccg atctccgggc
      121 tggccccggc ggcctgctcg atgtcgtcga agttcgggcc gccgagcacc gtcggatcgc
      181 cctcgaccgg gggcgcctcg ccgagccccg cgacgacgtc gatcttggcc tcggcggccg
      241 gcatgccgtc gatcagcccg tcgcggccga cccgcttgta ctgctcgagc tgctggcgct
      301 cgcggtcgat ctcgccggcg aacacgtcct tgagccagct cgacagcgac ttggccgtga
      361 acacggcgtc ctggctcatc aggaacgcct ggagatccgc gagcatctcg ctgcaccact
      421 ggtagcgctc ctcgacgtcc ttcgcgagcg ccttcatgat caccgcctcg accgcgggcg
      481 ggatgttcgg gttgatctcg cgcggcgggc ggacgtcgac gttgcgcacc ttctcgagcg
      541 tcgagaagtc ggtctcgccc tggaacagcc ggtcgctggt caggcactcg tagagcatcg
      601 tgccgagcgc gaagaggtcg ctgcggcgat cgagcggcag gccgcggacc tgctccggcg
      661 acatgtaccc gaacttgccc ttgagcacgc cggccatcgt ccgggagttg cgcgatgtcg
      721 ccttggcgat cccgaagtcg atgaccttga cctcgccctc gtacgacacc agcacgttct
      781 gtggcgagca gtcgcggtgg acgatctcca gcggccggcc gagcgggtcg cgcttgcggt
      841 gcgcgtagtc cagtccctcc agcaccttgg cgatgacgaa gcacgccatc gggatcggca
      901 tcggctgctt gatcttgcgc agccggttct ggatctggag cagatccttg ccccagatgt
      961 actccatcgc gatgaagtgc gagccgtcga tccggccgag ctcgaagatc tggcagatgt
     1021 tcgcgtgggc cagctggccg gcgatcttcg cctcgtcgat gaacatcttg atgaagtcgc
     1081 ggtcctcgcc catcgtgggc aagatccgct tgatcgcgat gatcttctcg aagccctcca
     1141 cgccatacga cttggccttg aacacctcgg ccatgccgcc cacgctgatg cgatcgagca
     1201 gcaagtactt cccgaaaggt acgggctgcc tcacctggtt cgatggtagc ggacggtccg
     1261 caacgtcaca atggcgaaag gggggcatgt gacctggggt cagcgacaac acctgcagag
     1321 cgtacccgag gctgtggggt cacaagccca tggtctttcg acgacagcca ccatgtaggt
     1381 gatgtgtcca gctctggtcc agagtcagag gctgcttcaa gagcgcctag aatcgagcgt
     1441 ggatcccgac aacgctgccg tcggtcgaga ccgtgacgta cggacgaagc gcttgctcgc
     1501 gggagattcg ccgataacca cgtataccgt cgtacacgcc gaatgcgtat accacccaga
     1561 aaccgatccc gctcgcgata ttgaacggcc gcagtcgccg ggcgtcctgc gtggcgtcgt
     1621 gtccgggccc ggtcccatcg gtgcaggtca gctggccgcc gccggatccc tgggtgtgat
     1681 cacaccactg gcggagatac gagtaggtca cgaggttggt gatgaggagc gcaccgagca
     1741 cgccgccgag cacgtaggcc ttggtgtggt cgccgttcat gtactggccg gccggcggga
     1801 tcagggtgaa cagccacgac ggccgctccg gccggacgcg cagcgcgcgg agctcggcgg
     1861 cgtggcgcga cgcgacgtcg ttgaagaacg cgacgacgtc gggcggatac agcgccgggt
     1921 cgagccggcc gtcgggctcg atccgcaggt acgtcacgaa gtgctgctcg gcgctcgcgt
     1981 tgcgctgggc gaagaacgcg gcgatccccg ccaggcgatg ggcctcggcg aggtcggcga
     2041 ccggcagctg ctcccggagc agcggatcga cgagatccgc gacccgctgc cagtcgccgg
     2101 ccagcgccgc ggtgttgccc tcgcgcagga cgtccgacgg cgcgcgcccc ggctgggcat
     2161 gcccgacccc gggcgcggcc agcgcgacca cc
//
LOCUS       DASVOR010001672         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_309729,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001672 DASVOR010000000
VERSION     DASVOR010001672.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_309729"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..1366
                     /locus_tag="VF516_31265"
     CDS             191..1366
                     /locus_tag="VF516_31265"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8112270.1"
                     /translation="MAAAAVVGLVGAGAALHTGASPAHAAPTAAPAALTDSSIPDVAE
                     RVFDSVVNISTSHAVEAGPASFDPFFSDPFSPGFGDPGDRKAMSKGSGVIVTASGRIL
                     TNAHVVKDADDITVTLHDGNDYDAKVVGVDPKADLAVLQLKGTLPALKPVTFGDSSVL
                     RLGEVVLAIGDPFGVGKSVTMGIVSAKGRGGMGIEEYEDFIQTDAAINPGNSGGALVN
                     LRGELVGINTAIASRSGGYAGIGFAIPTNMARPIMEMLIKDGKVSRGYLGVSIGTATP
                     AIAKQEKLGTQHGAVVAAVDPNGPAARAGLSEGDVVVGLNGTEIKTGYVLRNTIAATR
                     PGSTVDLEVVHRNGNKATVKAKLGELPADNVVRARPAAKQRRPQQQGQQQPQNWQWP"
     gene            1493..2647
                     /locus_tag="VF516_31270"
     CDS             1493..2647
                     /locus_tag="VF516_31270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112271.1"
                     /translation="MHAIAVLIRIGAVAALAACTSWTSACTSAPGVSAAHRERLALIR
                     DTAAGMGITNAALIAGIAVSETHLAHCWSEATYACQGPDSPSCGGPVIAGSADGPCAA
                     MQGGLGLFQLDAGTWSDTLARYGDAILTVEGNTAQAVSFVIDQVVRDIPGATGWLAAA
                     DWINGVPLAAGDPVMAQWAQLLACRYNGCCAASALCTQRAGGYRDHALEVYGELGAAF
                     WRTSDRCAALPADGVIDQRTACYVAGGDPRSWHGEAAGYGGSRDWAPTGGPSLARWIL
                     RLPAAGRYRVEVYAAGGEATASYQVAHAGPNGATNGMTNGVTVDAIAVDQAAAAGFVA
                     LGDFDFAASGDEHVELDAAGAPGAPGQKLVFDAIRVTRVTVSDARGMLAP"
     gene            2644..>2866
                     /locus_tag="VF516_31275"
     CDS             2644..>2866
                     /locus_tag="VF516_31275"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112272.1"
                     /translation="MNHPEAALVIAALVIAVLVQAALMHSSYGVVLRGSGGNPKAVAR
                     AGWSLLKTKMVMFGLAGTFGVLSGLSLIGL"
BASE COUNT          403 a         1006 c         1068 g          389 t
ORIGIN      
        1 cgggggagcg gatttcctgt acgggggcct tgacctggat cgcgagcgtg aacaagcaga
       61 tcgcggccct ccaaaaacac cgccggatcc cgtccgatcg ggcggccggg tcgtggaata
      121 gaggcaaccc gttctccggt ccaagattcg taactcacga ggaaccaaga tgaccccgct
      181 acgtactctc ttggctgcgg ctgctgtcgt tggactggtc ggcgctggcg ccgcgctgca
      241 caccggggca agcccggcgc atgcagcgcc gacggccgcg cccgccgcgc tgaccgatag
      301 ctcgatcccc gacgtcgccg agcgcgtgtt cgacagcgtc gtcaacatct ccacgtcgca
      361 cgcggtcgag gctggcccgg cctcgttcga tccgttcttc tcggatccgt tctcgccggg
      421 gttcggcgat cccggcgatc gcaaggcgat gagcaagggc tcgggcgtga tcgtcaccgc
      481 gagcggccgg atcctgacca acgcccacgt cgtcaaggac gccgacgaca tcacggtcac
      541 gctgcacgac ggcaacgact acgacgccaa ggtggtcggg gtcgacccca aggcggacct
      601 cgcggtgctg cagctcaagg gcaccctgcc cgcgctcaag ccggtcacgt tcggcgactc
      661 gtcggtgctc cggctcggcg aggtcgtgct cgcgatcggt gacccgttcg gcgtcggcaa
      721 gtcggtgacc atgggcatcg tctcggccaa gggccgcggc ggcatgggca tcgaggagta
      781 cgaggacttc atccagaccg acgccgcgat caacccgggc aactcgggcg gggcgctggt
      841 caacctccgg ggcgagctcg tcgggatcaa caccgcgatc gcgtcgcgga gcggcggcta
      901 cgccggcatc gggttcgcga tcccgaccaa catggcgcgg ccgatcatgg agatgctgat
      961 caaggacggc aaggtgtcgc gcggctacct cggggtcagc atcgggacgg cgaccccggc
     1021 gatcgccaag caggagaagc tcgggacgca gcacggcgcg gtcgtcgcgg ccgtcgatcc
     1081 caacgggccg gccgcgcggg ccgggctgtc cgagggcgac gtggtggtcg gcctcaacgg
     1141 caccgagatc aagaccggct acgtgctccg caacacgatc gcggcgacca ggccgggcag
     1201 caccgtcgac ctcgaggtcg tccaccgcaa cggcaacaag gcgacggtca aggccaagct
     1261 gggcgagctg ccggcggaca acgtcgtccg cgcccgcccg gcggccaagc agcgccggcc
     1321 gcagcagcag gggcagcagc agccgcaaaa ctggcagtgg ccgtagccgc ggcgcccgtg
     1381 cgctgaggca ctgcgacgcc gtgggtcccc cttgcgggac cgcggcgtcg gtcgttttcg
     1441 gctggcgccg ggcgccgcca ccggctgcgg cgcgcggggc tacactgggg gcgtgcacgc
     1501 gatcgcggtg ctgatccgga tcggggcggt cgcggccctg gcggcctgta cgtcctggac
     1561 gtcggcctgc acgtcggccc ccggggtgtc ggcggcgcat cgcgagcggc tcgcgctgat
     1621 ccgcgacacc gccgcgggga tgggcatcac caacgccgcg ctgatcgccg ggatcgcggt
     1681 cagcgagacc catctggcgc actgctggtc ggaggcgacc tatgcgtgcc aggggccgga
     1741 ctcaccgtcg tgcggtgggc cggtcatcgc gggctcggcg gacggcccgt gcgccgcgat
     1801 gcagggcggg ctcggcctgt tccagctcga cgccggcacc tggtccgaca ccctcgcgcg
     1861 ctacggcgat gcgatcctca ccgtcgaggg caacaccgcg caggcggtga gcttcgtgat
     1921 cgatcaggtg gtgcgcgaca tccccggcgc gaccggctgg ctggccgcgg cggactggat
     1981 caacggcgtg ccgctcgcgg ccggcgatcc ggtgatggcg cagtgggcgc agctgctggc
     2041 gtgccgctac aacggctgct gcgcggcgag cgcgctgtgc acccagcgcg ccggcggcta
     2101 tcgcgaccac gcgctggagg tgtacggcga gctcggcgcc gcgttctggc ggacgtcgga
     2161 tcgctgcgcc gcgctgccgg ccgacggcgt catcgatcag cggaccgcgt gctacgtcgc
     2221 cggcggtgac ccgcgcagct ggcacggcga ggccgccggc tacggcggct cgcgcgactg
     2281 ggcgccgacc ggcggaccga gcctcgcgcg ctggatcctc cggctgcccg cggccgggcg
     2341 ctaccgggtc gaggtctacg ccgcgggcgg cgaggcgacg gcgagctacc aggtcgcgca
     2401 cgccgggccg aacggagcga cgaacgggat gacgaacgga gtaacggtcg acgcgatcgc
     2461 ggtcgaccag gcggcggcgg ccgggttcgt cgcgctcggc gacttcgact tcgcggccag
     2521 cggcgacgag cacgtcgagc tcgacgcggc gggcgccccg ggcgcgcccg gccagaagct
     2581 ggtattcgat gcgatccggg tcacccgggt caccgtgtca gacgcccgcg gcatgctggc
     2641 gccgtgaacc acccggaggc tgcgctggtg atcgccgcgc tggtgatcgc cgtgctggtg
     2701 caggcggcac tcatgcactc ctcctatggg gtggtcctgc gcggcagcgg cggcaatccc
     2761 aaggccgtcg cccgcgccgg ctggtcgctg ctgaagacga agatggtgat gttcggcctc
     2821 gccggcactt tcggcgtgct ctccggcctc tcgctcatcg gtctca
//
LOCUS       DASVOR010001673         2388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_338285,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001673 DASVOR010000000
VERSION     DASVOR010001673.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2388
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338285"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VF516_31280"
     CDS             complement(<1..572)
                     /locus_tag="VF516_31280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112273.1"
                     /translation="MSRSAIIVDRFAPEIRALRAVFDARFAEPRVARADRFVWDYWHV
                     PDQYTALRTPAWTYFPRRLYAQFHHRLVAWGRAHLGCHDISPPWLSLYVEGCRQELHG
                     DLPHGPWAFVVSLTRWRGRRFRGGETLLLRDEVLDFWYDFASVRAVEQAALIRRIAPE
                     LGRLTVFDPRIPHGVRAVTGTHDPREGRLV"
     gene            complement(710..952)
                     /locus_tag="VF516_31285"
     CDS             complement(710..952)
                     /locus_tag="VF516_31285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112274.1"
                     /translation="MRNIIRTALVIGTLATPMITMADTPKKDAPAAKDTKDAKATDAK
                     DTKATDAKDAKAPAKKDAKAPAKKDTAKDAKDPAAK"
     gene            1205..>2388
                     /locus_tag="VF516_31290"
     CDS             1205..>2388
                     /locus_tag="VF516_31290"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HEX8112275.1"
                     /translation="MHRRLVCLLLSLLGIAAVVVAKVAAAHPHLRHATGTLSVEVRDE
                     NNGMLPARLTFRPVGDTPALYFTTIDIAREEAGAITAFDRAFVQRGDADIRVPAGTYD
                     VWISHGPEWDTSRERVTIKPGAEVEIHPRLHHVIDTPGWISADLHVHSAASLDSRVPM
                     RDRVLQFVADGVDLIVSTDHNVIADYAPVIAELGVGDLLASATGDEITTKQWGHFGAF
                     PLPADDGELGRGAIETGARSAAQIFADVRAKTPGALIDVHHPRLLHGSMGYFALADFD
                     LATGRTRTPGFSLDFDAIEVLNGYQDSDRRTMDKVISDWIGLLDGGKRVSCTGNSDSH
                     HMTFNLGGYPRNYVAVGDVPVARLDPAAVAAAVKAGHSFFTTGPILDAAIAGKGFGDV
                     ATV"
BASE COUNT          333 a          863 c          817 g          375 t
ORIGIN      
        1 atcacgagcc ggccctcgcg cggatcgtgg gtgccggtga ccgcgcggac gccgtgcggg
       61 atccgcgggt cgaacacggt gagccggccg agctcgggcg cgatccgccg gatcagcgcg
      121 gcctgctcga cggcgcggac cgatgcgaag tcgtaccaga agtcgagcac ctcgtcgcgc
      181 agcagcaggg tctcgccgcc gcgaaaccgc cggccgcgcc accgggtcag cgagaccacg
      241 aacgcccacg ggccgtgcgg caggtcgccg tgcagctcct gtcggcagcc ctcgacgtac
      301 agcgacagcc agggcggcga gatgtcgtgg cagcccaggt gggcgcggcc ccaggcgacg
      361 agccggtggt gaaactgcgc gtacaaccgc cgcggaaaat acgtccacgc cggcgtgcgc
      421 agcgcggtgt actggtccgg cacgtgccag tagtcccaca cgaagcggtc ggcccgggcc
      481 acgcggggct cggcgaaccg cgcgtcgaac accgcgcgca gcgcgcggat ctcgggcgcg
      541 aatcgatcga cgatgattgc gctgcgcgac acaggtcggt gatacccgaa aacggagatc
      601 ccattccgcg ccggggccgc cggggggcaa aaaaacccgt gacccgaaaa cgcgaacacc
      661 gccgttgccg gcggtgccaa cgtgggtggt agccgaggct accgggccat cacttggcgg
      721 ccgggtcctt cgcgtccttg gccgtgtcct tcttggccgg ggccttcgcg tccttcttgg
      781 ccggggcctt cgcgtccttg gcgtcggtcg ccttggtgtc cttggcgtcg gtcgccttcg
      841 cgtccttggt gtccttggcg gccggggcgt ccttcttcgg ggtgtctgcc atcgtgatca
      901 tcggggtggc gagcgttccg atgaccaggg cagtgcggat gatgttgcgc atgtggggat
      961 ctcctcgagt ttgattgctg aaagtcagcg acccgtcgtc gtgcacccgc tgtgcccgcc
     1021 ggcgcgccgc gatttcagcg ggttggcggg gctgatttcg gggcacgctg cccccgcggg
     1081 gcaaacggcc ccgagcctgc caccgcgttg aatcgtgacc gcagctcgtg cgtccttcac
     1141 gatcgccccg catcatcggt tcttcggtcc tcctcctctt cggtcctcgt cttcaggcgc
     1201 tcccgtgcac cgccggctcg tctgtctgct gctcagcctc ctcggcatcg ccgctgtcgt
     1261 cgtcgccaag gtcgccgctg cgcaccctca cctccgacat gcgaccggca cgctgtcggt
     1321 cgaggtccgc gacgagaaca acggcatgct cccggcgcgc ctgacgtttc gcccggtcgg
     1381 cgacactccc gcgctgtact tcacgacgat cgatatcgcc cgggaagagg ccggggcgat
     1441 cacggcgttc gaccgcgcgt tcgtccagcg cggcgacgcc gacatccgcg tcccggccgg
     1501 gacctacgat gtctggatca gccacggtcc ggagtgggac acctcgcgcg agcgggtcac
     1561 gatcaagccg ggcgccgagg tcgagatcca cccccggctg caccacgtga tcgacacgcc
     1621 gggctggatc tcggccgacc tccacgtcca ctcggcggcg tcgctcgact cccgcgtgcc
     1681 gatgcgcgac cgcgtgctgc agttcgtcgc cgacggcgtc gatctgatcg tctcgaccga
     1741 tcacaacgtg atcgccgact acgcgccggt gatcgccgag ctcggcgtcg gcgacctgct
     1801 ggcgtcggcg accggcgacg agatcacgac caagcagtgg ggccacttcg gcgcgttccc
     1861 gctgcccgcc gacgacggcg agctcggccg cggcgcgatc gagaccggcg cccgctcggc
     1921 ggcgcagatc ttcgccgacg tccgggccaa gacgcccggc gcgctgatcg acgtccacca
     1981 tccgcgcctg ttgcatggct cgatgggcta cttcgcgctc gccgacttcg atctcgccac
     2041 cgggcggacc cggaccccgg ggttctcgct ggacttcgac gcgatcgagg tcctcaacgg
     2101 ctaccaggac tcggatcgcc ggacgatgga caaggtgatc agcgactgga tcggcctgct
     2161 cgacggtggc aagcgggtca gctgcaccgg caactccgac tcgcaccaca tgacgttcaa
     2221 cctcggcggc tatccgcgca actacgtcgc ggtcggcgac gtcccggtcg cccggctcga
     2281 tccggcggcg gtcgcggcgg cggtcaaggc cggacacagc ttcttcacca ccgggccgat
     2341 cctcgacgcc gcgatcgccg gcaagggctt cggcgacgtc gcgaccgt
//
LOCUS       DASVOR010001674         5119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_425632,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001674 DASVOR010000000
VERSION     DASVOR010001674.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425632"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1633)
                     /locus_tag="VF516_31295"
     CDS             complement(107..1633)
                     /locus_tag="VF516_31295"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8112276.1"
                     /translation="MRYEVKIGWRYLYGGRSDGLMLRLGLVALGGVGLGAGLFTLSHG
                     SSALAVVILVLSMILAAAFFQLAVFSVFTSVSVLGVVLGVAALTFVLAITTGFEKQFR
                     DKVLGVNAHVIVLKSQATFAEYRDVMKTAREIDRDVLAVQPFIFAEMLATRGKGELSG
                     VAIKGIDPKLVRGVLDLDRHMIEGSVDSLLLEACPPGTDERTPGTGERPGAGLPGLRG
                     APTGPPPGVITATEPDRPRCLKDPPIIMGKELAHKLKARIGDTVTVVVPLSNIDFDTW
                     RAKTSAPRTRKFTVTGIFYSGFDEYDRRLMYASLTEVEELQGRGDQVMGVELKVRDVE
                     RAEQIAHKLEKALGGPPYQVQDWYELNHNLFTALTLQKLVLVLILTLIIVVAAVNMVS
                     ALTMLVMEKVREIAILKSMGASSSSVAVVFQVVGIAIGGAGTVLGVAIGLVMCYVASR
                     YGYRLDPKVYLIDRLPIEVKPLEVLLVAGITMAISLIATVVPARSAAALRPVDGLRYD
                     "
     gene            complement(1715..2299)
                     /locus_tag="VF516_31300"
     CDS             complement(1715..2299)
                     /locus_tag="VF516_31300"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HEX8112277.1"
                     /translation="MVSGAGRLYVDGDSIDGSAPLVFTLADLFSEAECEAEIARAEQL
                     GFAAAPITTAAGFEMRPDVRNNDRAMFDDVALATALFEQIREAVPRRLCSRRPVGVNE
                     RFRCYRYEPGQRFAPHYDGAFQRNRRERSELTLMIYLNDGFTGGQTTFHDFDVDVKPR
                     RGMALLFQHQVLHEGCAVESGVKYVLRTDVMYGD"
     gene            2436..3212
                     /locus_tag="VF516_31305"
     CDS             2436..3212
                     /locus_tag="VF516_31305"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8112278.1"
                     /translation="MPEARLDPTRAPRTQQQRREETRRALLDAAVQSLIEVGFARTTT
                     LEVQRRADVSRGALLHHFPSKAELLVAAVDHLAEMRAREMKAFASQLPPERPERSDRG
                     DREAGAHGEPVPGGDPRTGAVLDLLWQCFSGTFFKVSMELRTAARTDPELRRVLTAAE
                     RSLRDRIVAQSRTLFGRAVADHPGLERALDLTLQLMIGAAMTSVLHGQSSQLDDLIDD
                     WKALFPLVLANASARGQASPQGAPSEPLPLTDRKKLKGKR"
     gene            3209..>5119
                     /locus_tag="VF516_31310"
     CDS             3209..>5119
                     /locus_tag="VF516_31310"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8112279.1"
                     /translation="MTTLRFDGRVAIVTGAGGGLGRSHALLLASRGARVVVNDLGGSH
                     TGEGKSASAADKVVAEIKAAGGEAVASYDSVEDGDKIVKTAIDAFGKIDIIINNAGIL
                     RDVSFQKMTQADWDLVYRVHVLGAFRVTHAAWNHMRDAGYGRIVNTSSASGIYGNFGQ
                     ANYSMAKLGLHGFTQTLALEGKKRNIIVNTIAPTAGSRMTETVMPPALVEALRPELVS
                     PLVARLVHESQEDTGGLYEVGGGFFAKLRWERSTGKTFRLGRSIALEDVDAAMPQITQ
                     FDRTTHPENITAALQPLIENVEAGPSKGGNQFIDVDAALGHKYPEFQSSYDERDVALY
                     ALGVGAARSSTDARDLQLVYELDGRGMKVLPSFGVLPAINSVFARAKQGINAPGLTFG
                     LDRVLHGEQYTELKRPLPTRAKLVTRGTVKSIYDKGKGAVVNTEFITYDEHGDELIRN
                     DIATFVRGAGGWGGERGPSADVNVPPDRAPDKVVEDKTSENQALLYRLSGDWNPLHAD
                     PSFAKAFGFERPILHGLCFFGFVTRHVAQAFAPDGNPDHVKSIRVRFAASMLPGQTLI
                     TEMWKDSDTRIIFRCKIKETGEVCISNAAIELWKELPKPKERAKPAAGAAASAGPAAA
                     TSGDIFRAIGAFV"
BASE COUNT          826 a         1847 c         1744 g          702 t
ORIGIN      
        1 agggtcgcgg ccgcgcgcgc cgcggtcgac gagctgtgcg gcgacatccg cgagctgctc
       61 ggcgtggggt gacgcgctcg gcgtgcgccg cggaccggcg tgacgatcag tcgtagcgca
      121 gcccgtcgac cggccgcagc gcggcggccg accgcgccgg caccaccgtc gcgatcaggc
      181 tgatcgccat cgtgatcccg gcgaccagca gcacctcgag cggcttgacc tcgatcggca
      241 gccggtcgat caggtagacc ttggggtcga ggcggtagcc gtagcgcgac gccacgtagc
      301 acatgaccag gccgatcgcg acgccgagca ccgtgccggc gccgccgatc gcgatgccga
      361 cgacctggaa caccaccgcc acgctcgacg acgacgcccc catcgacttg aggatcgcga
      421 tctcgcgcac cttctccatc accagcatgg tcagcgccga caccatgttg accgccgcga
      481 ccacgatgat cagcgtcagg atgagcacca gcaccagctt ctgcagggtc agcgcggtga
      541 acaggttgtg gttgagctcg taccagtcct ggacctggta cggcggcccg ccgagcgcct
      601 tctccagctt gtgggcgatc tgttcggcgc gctcgacgtc gcggaccttg agctcgaccc
      661 ccatgacctg atcgccccgg ccctgcagct cctcgacctc ggtcagcgac gcgtacatca
      721 gccggcggtc gtactcgtcg aacccgctgt agaagatccc ggtgacggtg aacttgcggg
      781 tccgcggcgc gctggtcttc gcgcgccagg tgtcgaagtc gatgttcgac agcggcacca
      841 cgaccgtgac cgtgtcgccg atcctggcct tgagcttgtg cgccagctcc ttgcccatga
      901 tgatcggcgg gtccttcagg caccgcggcc ggtcgggctc ggtcgcggtg atcacccccg
      961 gcggcgggcc cgtcggcgca ccccggagcc caggcaaccc cgcgcccggg cgctcaccgg
     1021 tgcccggggt gcgctcgtcc gtgccgggcg ggcacgcctc gagcagcagc gagtcgaccg
     1081 agccctcgat catgtgtcga tcgaggtcga gcaccccgcg caccagcttc ggatcgatgc
     1141 ccttgatcgc gacgccggac agctcgccct tgccgcgcgt cgccagcatc tcggcgaaga
     1201 tgaacggctg gaccgcgagc acgtcgcgat cgatctcgcg cgcggtcttc atcacatccc
     1261 ggtactcggc aaacgtcgcc tgcgacttga gcacgatcac gtgggcgttg acgcccagca
     1321 ccttgtcgcg gaactgcttc tcgaacccgg tggtgatcgc gagcacgaag gtcagcgcgg
     1381 cgaccccgag caccacgccg agcaccgaca cgctggtgaa cacggagaac accgcgagct
     1441 ggaagaacgc cgccgccagg atcatgctga gcaccaggat caccaccgcg agtgcgctgg
     1501 agccatgcga cagcgtgaac agccccgcgc cgagccccac gcccccgagg gccacgaggc
     1561 ccagccggag catcagcccg tcgctccggc cgccgtagag gtagcgccac ccgatcttga
     1621 cctcgtatct cacgtcggtc ccagcttgcc ccggaccgcc gaccggcgcc agccaggggt
     1681 cgtgctgatt tgcgcagcct cggtcacgcc cgctctagtc gccgtacatc acgtcggtgc
     1741 gcagcacgta cttgaccccg gactcgacgg cgcagccctc gtgcagcacc tggtgctgga
     1801 acagcagcgc catgccccgg cgcggcttca cgtcgacgtc gaagtcatgg aacgtggtct
     1861 gcccgccggt gaacccgtcg ttgaggtaga tcatgagcgt cagctcgctg cgctcgcggc
     1921 ggttgcgctg gaacgccccg tcgtagtgcg gcgcgaaccg ctggcccggc tcgtagcggt
     1981 agcaccggaa ccgctcgttg accccgaccg gccgccggct gcacagccgc ctgggcaccg
     2041 cctcgcggat ctgctcgaac agcgcggtcg cgagcgcgac gtcatcgaac atcgcccggt
     2101 cgttgttgcg aacgtcgggc cgcatctcga accccgcggc ggtcgtgatc ggcgcggcgg
     2161 cgaacccgag ctgctcggcc cgcgcgatct ccgcctcgca ctcggcctcg ctgaacaggt
     2221 cggcgagggt gaacaccagc ggcgcggatc cgtcgatgct gtcgccgtcg acatagagcc
     2281 ggccggcacc cgaaaccacg atatgcggac cctgcatccc ggtagtgtac ttacacacac
     2341 gaggaggcgg cgtgacgtgc cacccggcgc cgccgcgtcc gccatcatac aaaccgtctt
     2401 gactgactgt ttttggggcg gtaagacccc accggatgcc cgaggccagg ctcgatccga
     2461 cgcgcgcacc ccgcacccag cagcagcgcc gcgaggagac ccggcgcgcg ctgctcgacg
     2521 cggcggtcca gagcctgatc gaggtcgggt tcgcgcggac caccacgctc gaggtccagc
     2581 gccgggccga cgtgtcgcgc ggcgcgctgc tccaccactt cccgtccaag gccgagctcc
     2641 tggtcgcggc ggtcgaccac ctcgccgaga tgcgggcgcg cgagatgaag gcgttcgcgt
     2701 cgcagctgcc gcccgagcga ccggagcgca gcgaccgcgg cgaccgcgag gcgggagcgc
     2761 acggcgagcc ggtgcccgga ggcgatccgc ggaccggcgc ggtgctcgac ctgctctggc
     2821 agtgcttctc gggcacgttc ttcaaggtct cgatggagct gcgcaccgcg gcgcgcaccg
     2881 atccggagct ccgccgcgtg ctgacggcgg ccgagcgctc gctgcgcgat cggatcgtcg
     2941 cgcagagccg gacgctgttc ggccgcgcgg tcgccgacca ccccgggctc gagcgcgcgc
     3001 tcgacctgac gctgcagctg atgatcggcg cggcgatgac gtcggtgctg cacggccaga
     3061 gctcgcagct cgacgatctg atcgatgact ggaaggcgct gttcccgctg gtgctggcca
     3121 acgcgagcgc gcgcggccag gctagcccgc agggcgcgcc cagcgaacca ctaccactca
     3181 cggaccgcaa gaagctgaaa ggcaagcgat gacgacactc aggttcgacg gaagagttgc
     3241 gatcgtgacc ggggccggcg gcggcctcgg ccgctcccat gccctgctgc tcgcgtcgcg
     3301 tggcgcgcgg gtcgtggtca acgacctcgg cggcagccac accggcgagg gcaagtcggc
     3361 gtcggccgcc gacaaggtgg tcgccgagat caaggccgcc ggcggcgagg cggtcgcgag
     3421 ctacgactcg gtcgaggacg gcgacaagat cgtcaagacc gcgatcgacg cgttcggcaa
     3481 gatcgacatc atcatcaaca acgccgggat cctgcgcgac gtgtcgttcc agaagatgac
     3541 ccaggccgac tgggacctgg tctaccgggt ccacgtgctc ggcgcgttcc gggtcaccca
     3601 cgcggcgtgg aaccacatgc gcgacgccgg ctacggccgg atcgtgaaca cgtcgtcggc
     3661 gtcgggcatc tacggcaact tcggccaggc caactactcg atggccaagc tcggcctgca
     3721 cggcttcacc cagacgctcg cgctcgaggg caagaagcgc aacatcatcg tcaacacgat
     3781 cgcgccgacc gcgggctcgc ggatgaccga gaccgtgatg ccgccggcgc tggtcgaggc
     3841 gctgaggccc gagctggtct cgccgctggt cgcccggctg gtccacgagt cgcaggagga
     3901 caccggcggg ctgtacgagg tcggcggcgg gttcttcgcc aagctgcgct gggagcgctc
     3961 gaccggcaag acgttccggc tcggccgctc gatcgcgctc gaggacgtcg acgcggcgat
     4021 gccgcagatc acccagttcg acaggaccac ccaccccgag aacatcaccg cggcgctcca
     4081 gccgctgatc gagaacgtcg aggccggccc gagcaagggc ggcaaccagt tcatcgacgt
     4141 cgacgccgcg ctcggccaca agtaccccga gttccagtcg tcgtacgacg agcgcgacgt
     4201 ggcgctgtac gcgctcggcg tcggcgccgc ccggagctcg accgacgcgc gcgacctcca
     4261 gctggtctac gagctcgacg gccgcggcat gaaggtgctg ccgagcttcg gcgtgctgcc
     4321 ggcgatcaat tcggtgttcg cccgggccaa gcagggcatc aacgcgccgg gcctcacgtt
     4381 cggcctcgac cgcgtgctgc acggtgagca gtacaccgag ctcaagcggc cgctgccgac
     4441 ccgggccaag ctggtcaccc gcggcacggt caagagcatc tacgacaagg gcaagggcgc
     4501 ggtggtcaac accgagttca tcacctacga cgagcacggc gacgagctga tcaggaacga
     4561 catcgcgacc ttcgtgcgcg gcgccggcgg ctggggcggc gagcgcggcc cgagcgcgga
     4621 cgtcaacgtg ccgcccgacc gcgcgccgga caaggtcgtc gaggacaaga ccagcgagaa
     4681 ccaggcgctg ctgtaccggc tgtccggcga ctggaacccg ctgcacgccg atccgtcgtt
     4741 cgccaaggcg ttcgggttcg agcggccgat cctgcacggg ctgtgcttct tcgggttcgt
     4801 gacccgccac gtcgcgcagg cgttcgcgcc cgacggcaac cccgaccacg tgaagtcgat
     4861 ccgggtgcgg ttcgcggcca gcatgctgcc cggccagacc ctgatcaccg agatgtggaa
     4921 ggacagcgac accaggatca tcttccgctg caagatcaag gagaccggcg aggtctgcat
     4981 ctcgaacgcc gcgatcgagc tgtggaagga gctgcccaag cccaaggagc gcgccaagcc
     5041 ggcggccggc gcggcagcat cggcgggccc ggcggccgcg accagcggcg acatcttccg
     5101 cgcgatcggc gcgttcgtc
//
LOCUS       DASVOR010001675         3780 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_455346,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001675 DASVOR010000000
VERSION     DASVOR010001675.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455346"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="VF516_31315"
     CDS             complement(<1..278)
                     /locus_tag="VF516_31315"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEX8112280.1"
                     /translation="MADLVVPALGESITEAVVSIWIKQVGDAVAADEPVAELETDKVT
                     VQLPSPVAGVLAEQNVAVGATVRVGDVIGSVTPGAEKTAAKAAVPAQHV"
     gene            complement(384..3275)
                     /locus_tag="VF516_31320"
     CDS             complement(384..3275)
                     /locus_tag="VF516_31320"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEX8112281.1"
                     /translation="MVRDVLELGSSLGFLDEISEGFLQQPDAVDPSWRSLLTEHPPVV
                     GDGHRPAAGGNGHPSSGRAQTLVAELQTLPPVSTRPVTMPLSPVVTAISSPAAPMVWP
                     LVNAYRSRGHFNAKLDPLGLLETARIVELDPATWGFTPRDFNRLIEPTGVHGLARATL
                     GEILAHIQRVYASTVGLEFMHITSPARRSWLAERMETQLVAPLPAETRIRMLAQLINA
                     EQFERFCHTKYPGTKRFSLEGSEGLIPVLDAILTHTARLGAIEAVLGLAHRGRLTTLE
                     QILRRPGRDLFAQFEDVEPEKAMGGGDVKYHLGFSTDRADANGHVMHVSLAFNPSHLE
                     AVDPVVVGRVRAKQIRHGDFEHRRVVGVLVHGDAAFAGQGLVPETLQLSGLPGYRTGG
                     TIHVIANNQVGFTASPAEQRSTPYCTDVAKMLECPIWHVNGEDIDALARVVEIACEYR
                     AQFASDVVIDVYCYRKYGHNEADEPSFTQPLMYERIKRKASPVEVYTRQLIEQGVVST
                     DEVAAMTQRRVAELEAELEAARAAKQRPDAPSMTAMWRGYRGGISEHPEDTDTRVPRA
                     VLEHIASSMAELPPGFTPHPKIERLLEQRAQMGKGQRPIDWGMAELLAYGSLLHQKIN
                     VRMSGQDCSRGTFSHRHAIITDIKTGREHLVLGQLHADQGLCRIFDSPLSEAGVLGFE
                     FGYSLDYPDSLVLWEAQFGDFANGAQVIIDQFIVASEDKWKRLSGIVLLLPHGYEGQG
                     PEHSSARLERYLQSCAEHNLQVAQPTTPAQMFHLLRSQVLCPLRKPLVVMTPKSLLRL
                     PAATSTLDELASGRYRRVLADEAADPAKVTRVLVCTGKIYYELADERARRADPTTAIV
                     RLETLYPWWPQLVAAATPERYPKLAELFWVQDEPANMGAATFVAPRIQALIAGRGVRY
                     EMISRAESASPATGSHKAHVIEQQEILGKAFGRSGATAVAGAGRAAGTS"
     gene            complement(3327..>3780)
                     /locus_tag="VF516_31325"
     CDS             complement(3327..>3780)
                     /locus_tag="VF516_31325"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8112282.1"
                     /translation="VGAVWLLALLGYNISIAVWVGMIALMGLDAETGVFMLLFLDLSH
                     DEARRAGRLRSEAELVEAIIHGAVKRVRPKAMTVCAAMLGLLPILWSAGTGADVMKRI
                     AAPMVGGLVTSFAMELLVYPPIYFLWRRRQLSATAEVDAAAAVAPAAP"
BASE COUNT          525 a         1387 c         1318 g          550 t
ORIGIN      
        1 acatgctgcg cagggaccgc cgccttcgcc gccgtcttct ctgctccggg cgtgacgctg
       61 ccgatcacgt cgccgacccg caccgtggcg ccgaccgcga cgttctgctc ggccagcacg
      121 ccggcgaccg gcgacggcag ctgcaccgtg accttgtcgg tctcgagctc cgcgaccggc
      181 tcgtcggcgg ccaccgcgtc gccgacctgc ttgatccaga tcgagaccac ggcctcggtg
      241 atcgattcgc ccagcgcagg gacgacgaga tctgccatgc ggcgcatgct acccggtcag
      301 ctgcagccgc gcaccgcgcg cgtgccccag atccggtccg accgaaccgc ccgacgagcc
      361 gtgatgcccg ccgcgccgaa gggtcagctc gtcccggccg cgcggcccgc tccggccacg
      421 gcggtcgctc cgctccggcc gaacgccttg ccgaggatct cctgctgctc gatgacgtgc
      481 gccttgtgcg agccggtggc agggctggcg ctctcggcgc ggctgatcat ctcgtacctg
      541 acgccgcgcc cggcgatcag cgcctggatc cgcggcgcaa cgaacgtcgc cgcccccatg
      601 ttcgccggct cgtcctggac ccagaacagc tcggcgagct tgggatagcg ctcgggggtc
      661 gccgccgcca ccagctgcgg ccaccacgga tacagcgtct cgagccggac gatcgcggtg
      721 gtcgggtcgg cgcgccgcgc gcgctcgtcg gccagctcgt agtagatctt gccggtgcac
      781 accagcaccc gcgtgacctt ggccggatcg gcggcctcgt cggccagcac ccggcggtag
      841 cggccgctcg cgagctcgtc gagcgtcgac gtcgccgccg gcaggcggag caggctcttg
      901 ggcgtcatca ccaccagcgg cttgcgcagc gggcacagca cctggctgcg cagcaggtgg
      961 aacatctgcg ccggcgtggt cggctgcgcg acctggaggt tgtgctcggc gcacgactgc
     1021 aggtagcgct cgagccgcgc gctcgagtgc tccggcccct ggccctcgta gccgtgcggc
     1081 agcagcagca cgatgcccga cagccgcttc cacttgtcct ccgacgcgac gatgaactga
     1141 tcgatgatca cctgggcgcc gttggcgaag tcgccgaact gcgcctccca cagcacgagc
     1201 gaatcggggt agtcgagcga gtagccgaac tcgaagccga gcacgccggc ctccgacagc
     1261 gggctgtcga agatccggca cagcccctgg tcggcgtgga gctggccgag caccaggtgc
     1321 tcgcggccgg tcttgatgtc ggtgatgatc gcgtggcggt ggctgaacgt gccgcgcgag
     1381 cagtcctgcc cgctcatccg cacgttgatc ttctggtgca gcagcgaccc gtacgccagg
     1441 agctccgcca tcccccagtc gatcggccgc tggcccttgc ccatctgcgc ccgctgctcg
     1501 agcagccgct cgatcttggg gtgcggcgtg aacccgggcg gcagctcggc catgctcgac
     1561 gcgatgtgct ccagcacggc gcgcggcacc cgggtgtcgg tgtcctcggg gtgctcggag
     1621 atgccgccgc ggtagccgcg ccacatcgcg gtcatcgacg gcgcgtccgg gcgctgcttg
     1681 gccgcgcgcg cggcctcgag ctcggcctcg agctcggcga cccggcgctg ggtcatcgcc
     1741 gcgacctcgt cggtcgacac cacgccctgc tcgatcagct gccgggtgta gacctcgacc
     1801 ggcgacgcct tgcgcttgat ccgctcgtac atcagcggct gggtgaagct cggctcgtcg
     1861 gcctcgttgt ggccgtactt gcggtagcag tacacgtcga tcacgacgtc ggacgcgaac
     1921 tgcgcgcggt actcgcacgc gatctcgacc acgcgggcca gcgcgtcgat gtcctcgccg
     1981 ttgacgtgcc agatcgggca ctccagcatc ttggcgacgt cggtgcagta cggcgtcgag
     2041 cgctgctcgg ccggcgacgc ggtgaacccg acctggttgt tggcgatgac atggatcgtc
     2101 ccgccggtcc ggtagcccgg cagcccggac agctgcagcg tctcgggcac caggccctgg
     2161 ccggcgaacg cggcgtcgcc gtggaccagc acgccgacca cgcggcggtg ctcgaagtcg
     2221 ccgtgccgga tctgcttggc gcggacccgg ccgaccacca ccggatcgac cgcctcgagg
     2281 tggctcgggt tgaacgccag cgacacgtgc atcacgtggc cgttggcgtc ggcgcggtcg
     2341 gtcgagaacc cgaggtggta cttgacgtcg ccgccgccca tcgccttctc cggctcgacg
     2401 tcctcgaact gcgcgaacag atcgcgcccg ggccggcgca ggatctgctc cagcgtggtc
     2461 agccggccgc ggtgcgccag gccgagcacc gcctcgatcg ccccgagccg cgcggtgtgg
     2521 gtcaagatcg cgtcgagcac cgggatcagg ccctcgctgc cctcgagcga gaaccgcttg
     2581 gtcccggggt acttggtgtg gcagaaccgc tcgaactgct cggcgttgat cagctgcgcc
     2641 agcatccgga tccgggtctc ggcgggcagc ggcgccacca gctgggtctc catccgctcg
     2701 gcgagccacg accggcgcgc cggcgacgtg atgtgcatga actccagccc gacggtgctg
     2761 gcgtacaccc gctggatgtg cgcgaggatc tcgccgagcg tcgcgcgcgc cagcccgtgc
     2821 acgccggtcg gctcgatcag ccggttgaag tcgcgcggcg tgaaccccca ggtcgccgga
     2881 tcgagctcga cgatccgcgc ggtctccagc aggcccagcg gatcgagctt ggcgttgaag
     2941 tggccgcggc tgcggtacgc gttgaccagc ggccacacca tcggcgcggc cggagacgag
     3001 atcgccgtca cgaccggcga cagcggcatc gtcaccggcc gggtcgacac cggcggcagc
     3061 gtctgcagct cggcgaccag cgtctgcgcc cgcccgctgc tcgggtggcc attgccgcct
     3121 gccgcgggcc ggtggccgtc gccgacgact ggtggatgct cggtcaagag cgatcgccag
     3181 ctcggatcga ccgcgtcggg ttgctgcagg aagccttccg agatctcgtc gaggaagccg
     3241 agcgagctgc cgagctcgag gacgtcgcgc accatggccg gcatgatagc gccaatgcgc
     3301 ccgatgaccc gccttcgggc agattgtcac ggcgctgccg gcgccacggc ggctgccgcg
     3361 tcgacctcgg cggtagccga gagctggcgc cgccgccaca ggaagtagat cggcgggtag
     3421 accaggagct ccatcgcgaa gctggtgacc aggccgccga ccatcggcgc cgcgatccgc
     3481 ttcatgacgt cggcgccggt gccggccgac cacaggatcg gcaagaggcc cagcatcgcg
     3541 gcgcacaccg tcatcgcctt ggggcggacc cgcttgaccg cgccgtggat gatcgcctcg
     3601 accagctcgg cctcgctgcg cagccggccg gcgcgacggg cctcgtcgtg cgacaggtcg
     3661 aggaacagca gcatgaacac gccggtctcg gcgtcgagcc ccatcagcgc gatcatcccg
     3721 acccacacgg cgatcgagat gttgtagccg agcagcgcga gcagccacac cgcgccgacc
//
LOCUS       DASVOR010001676         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_75501,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001676 DASVOR010000000
VERSION     DASVOR010001676.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75501"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2183)
                     /locus_tag="VF516_31330"
     CDS             complement(<1..>2183)
                     /locus_tag="VF516_31330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112283.1"
                     /translation="EGLPPRAPVEQALRALIPATPAVVLDPLRPLLGKLLPSVAGPQA
                     VMFRDPGKEKIAVFEALSRWLRTLAEIGPFILAFEDLHWADSATLETMNVIIRALHGT
                     GGLVVATFRTDELGRLSLAFQTVDEGLTDHVELAPLSEHDLTTLVELALQGFHPGPAL
                     ARNLYEATRGNAFFATECLRALIEEGTLTRRLGTWTAAPELSHRRLPRSIREVVLARL
                     STLSGDQVAFFQRLAPAGRMLDVPLIRAVGDVSDRELFQLLDEGVERQFLQYVEGRYF
                     FTHATVHEAIYEGTPEPMRRRFHKRIAEHLVHSAGDRPEAARAIGYHFARSDEPTRAI
                     DPLLRAAARALDDKTMFDAFLLLEEVAGLLEANPDVPDRDARLIAVWGDLIEVGYTSS
                     TPTCIRYAQKLFQHWDATVDLARGQAEIKAALEAVIAAPTEHHTELLTELFPEIQIHA
                     ARHPRDVFLKRSEYRILESIALAITGRTAEFFAGLERTDQEHPAESPYRAATHVAIGG
                     LTSHTGHFRHAVDEMREHIAVLRGFIDLVAACPRRLQWALGMGAYFMNMNLALMGLPL
                     DQRATADGFAIAERLGFTDLRIYHLFSQIVRAAFIGDGSAYGPPFAEMNDLMRKLGNP
                     LLPARNLAIYTPPYYLERGELEMARAVIERGEHLARLLPGDRWLRLYVEVYRACLHVA
                     AGDAGAEAAIADTLAGARVSDFRMETLVLIYRARFERARGRPADA"
BASE COUNT          298 a          738 c          856 g          291 t
ORIGIN      
        1 ggcgtccgcc ggccggccgc gcgcgcgctc gaaccgggcc cggtagatca ggaccagggt
       61 ctccatccgg aaatcggaca cccgggcgcc ggcgagcgtg tcggcgatcg cggcctcggc
      121 gccggcgtcg ccggcggcga cgtggaggca cgcgcggtag acctcgacgt agagccggag
      181 ccagcggtcg ccgggcaaca gccgcgccag gtgctcgccg cgctcgatca ccgcgcgcgc
      241 catctcgagc tcgccgcgct cgaggtagta cggcggggtg tagatcgcca ggttgcgggc
      301 gggcagcagc gggttgccga gcttgcgcat caggtcgttc atctcggcga acggcggccc
      361 gtaggccgag ccgtcgccga tgaacgccgc gcggacgatc tgcgagaaca ggtggtagat
      421 ccgcaggtcg gtgaagccga gccgctcggc gatcgcgaac ccgtcggcgg tggcgcgctg
      481 gtcgagcggc agccccatca gcgcgaggtt catgttcatg aagtaggcgc ccatgccgag
      541 cgcccactgc agccgccgcg ggcaggcggc gaccaggtcg atgaacccgc gcagcaccgc
      601 gatgtgctcg cgcatctcgt cgaccgcgtg gcggaagtgc ccggtgtgcg aggtcagccc
      661 gccgatcgcg acgtgggtcg cggcgcggta cggcgactcg gccgggtgct cctggtcggt
      721 gcgctcgagg ccggcgaaga actccgcggt gcggccggtg atcgcgagcg cgatgctctc
      781 caggatccgg tactccgagc gcttgaggaa cacgtcgcgc gggtggcggg cggcgtggat
      841 ctggatctcg gggaacagct cggtgagcag ctcggtgtgg tgctcggtcg gcgcggcgat
      901 caccgcctcg agcgcggcct tgatctcggc ctggccgcgc gcgaggtcga ccgtggcgtc
      961 ccagtgctgg aacagcttct gggcgtagcg gatgcacgtc ggcgtgctgc tggtgtagcc
     1021 gacctcgatc aggtcgcccc acaccgcgat cagccgggcg tcgcggtcgg ggacgtcggg
     1081 gttggcctcg agcagcccgg cgacctcctc gagcaggagg aacgcgtcga acatggtctt
     1141 gtcgtcgagc gcgcgcgccg cggcgcgcag cagcgggtcg atcgcgcggg tcggctcgtc
     1201 ggaccgggcg aagtggtagc cgatcgcgcg cgcggcctcg gggcggtcgc ccgcgctgtg
     1261 gaccaggtgc tcggcgatcc gcttgtggaa ccgccggcgc atcggctcgg gggtgccctc
     1321 gtagatcgcc tcgtgcacgg tggcgtgggt gaagaagtag cggccctcga cgtactgcag
     1381 gaactggcgc tcgacgccct cgtcgagcag ctggaacagc tcgcggtcgg agacgtcgcc
     1441 gaccgcccgg atcagcggga cgtcgagcat ccggccggcg ggcgcgagcc gctggaagaa
     1501 cgcgacctgg tcgccggaca gggtcgacag ccgggcgagc acgacctccc ggatgctgcg
     1561 cggcagccgg cgatgtgaca gctcgggcgc cgcggtccag gtgccgagcc ggcgggtcag
     1621 ggtgccctcc tcgatcaggg cgcgcaggca ctcggtcgcg aagaacgcgt tgccgcgggt
     1681 cgcctcgtac aggttgcggg ccagcgccgg cccggggtgg aagccctgga gcgcgagctc
     1741 gaccagcgtg gtcaggtcgt gctcggacag cggcgcgagc tcgacgtgat cggtcaggcc
     1801 ctcgtcgacg gtctggaacg cgagcgacag ccgcccgagc tcgtcggtgc ggaacgtcgc
     1861 gaccaccagg ccgccggtgc cgtgcagcgc gcggatgatc acgttcatgg tctcgagggt
     1921 cgcgctgtcg gcccagtgca ggtcctcgaa cgccaggatg aacgggccga tctcggcgag
     1981 cgtgcgcagc cagcgcgaca gcgcctcgaa caccgcgatc ttctccttgc ccggatcgcg
     2041 gaacatcacg gcctgtggcc cggcgacgct cggcagcagc ttgccgagca gcgggcgcag
     2101 cgggtcgagc acgaccgccg gggtggccgg gatcagcgcg cgcagcgcct gctcgaccgg
     2161 cgcgcgcggc ggcagcccct cgg
//
LOCUS       DASVOR010001677         1783 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_251364,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001677 DASVOR010000000
VERSION     DASVOR010001677.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251364"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1172
                     /locus_tag="VF516_31335"
     CDS             141..1172
                     /locus_tag="VF516_31335"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HEX8112284.1"
                     /translation="MKRLLLVCVLVACSANVQRPVAPGGPVPVSTRPEPAAAAPSRVV
                     TEHFHSDALGVDKAVVIYLPRGYDSQPARRWPVFYYLHGLGGNETNWVKRGKLDAAAD
                     QLGLAAIVVMPDGDDGFYIDSRAPIDHDQCMKDGSGLFVPGREPHDAACVHARNYETY
                     IAKDLVGWVDGHYHTIARRDGRAVAGLSMGGFGAMELALRHPGEFAAAGSHSGAIALL
                     YLGPRPFAPGKAELLTDASSFGREGGPIVAWIHTLFGDDIADWKAHDVVELAGKLGPG
                     KVALYFDCGTEDDFALQDNVQYVHEALGARHIEHAFFVGPGKHDFAFWSVRVASSLAF
                     LRDHTAKPE"
     gene            1575..1721
                     /locus_tag="VF516_31340"
     CDS             1575..1721
                     /locus_tag="VF516_31340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112285.1"
                     /translation="MIYPWRAPFVLDDAQFRARFGATPTPVARAVEATVAWARARYTV
                     RAAA"
BASE COUNT          219 a          693 c          618 g          253 t
ORIGIN      
        1 ggcgccggcg acgccgcgct gctgcgcgcg ctgaccaccg tcgatcccgt cctggccgcg
       61 gccaccgagc gcctggtcca gctcgtgctc tcgttccgct cgtcgacgat ccaggccgtc
      121 gcgatgctcc cggtgatcca atgaagcgct tgctcctggt ctgcgtcctc gtcgcgtgtt
      181 cggccaatgt ccagcgcccg gtcgcaccgg gtggaccggt cccggtatcc actcggcccg
      241 agccggccgc cgcggcgccg agccgcgtcg tcaccgagca cttccacagc gacgcgctcg
      301 gcgtcgacaa ggcggtcgtg atctacctgc cgcgcggcta cgacagccag ccggcccggc
      361 ggtggccggt gttctactac ctgcacgggc tcggcggcaa cgagaccaac tgggtcaagc
      421 gcggcaagct cgacgccgcg gccgatcagc tcgggctcgc cgcgatcgtg gtgatgcccg
      481 acggcgacga cggcttctac atcgactcgc gggcgccgat cgaccacgac cagtgcatga
      541 aggacggcag cgggctgttc gtccccggcc gcgagccgca cgacgccgcg tgcgttcacg
      601 cgcgcaacta cgagacctac atcgcgaagg acctggtcgg ctgggtcgac ggccactacc
      661 acacgatcgc gcgccgcgac ggccgcgccg tggccgggct gtcgatgggc gggttcggcg
      721 cgatggagct cgcgctgcgc caccccggcg agttcgccgc tgccggcagc cactcgggcg
      781 cgatcgcgct gctctacctt gggccgcggc cgttcgcgcc cggcaaggcc gagctgctca
      841 ccgacgcgtc gagcttcggc cgggagggcg gcccgatcgt cgcgtggatc cacacgctgt
      901 tcggcgacga catcgccgac tggaaggccc acgacgtcgt cgagctcgcc ggcaagctcg
      961 gcccgggcaa ggtcgcgctg tacttcgact gcggcacgga ggacgacttc gcgctgcagg
     1021 acaacgtcca gtacgtccac gaggcgctgg gcgcccggca catcgagcac gcgttcttcg
     1081 tcggccccgg caagcacgac ttcgcgttct ggtcggtgcg ggtggcgagc agcctggcgt
     1141 tcctgcgcga tcacaccgcg aagcccgagt agcccgcgcc gcgcggcgat ctcccggtcg
     1201 cgatcggccg cgcggccgac ttcttcggcc cgggatgcac caactcgatc ttcggcgagc
     1261 ggttctggac caagctgctc gccggccggc cggtcgagct gctcggcgat cccgaccagc
     1321 cgcacaccta ctcgtacggc cccgacgtcg ccgacgcgct ggtcaccctc ggcgtcgccg
     1381 atccggcgct gtcgagcggc gccgatgtct acggccgggt ctggcacctg ccggcgcatc
     1441 cggccgagtc gacccaggcg catctcgcgc ggttcgcccg cgcggcgggc gtcgagccgc
     1501 ggttcacccg gctgtcgccg ctcctgctcc ggatcgccgg cctgttcctg cccgaggccg
     1561 gcgagctgcc cgagatgatc tacccgtggc gcgcgccgtt cgtcctcgac gacgcgcagt
     1621 tccgcgcccg gttcggcgcg accccgaccc cggtcgcgcg cgcggtcgag gccaccgtcg
     1681 cctgggcgcg cgcccgctac accgtgcgcg cggccgcctg acgccgcgat ctgcaagcgc
     1741 ccccccgggg cgagccgctg gatccacccc ggcggctgga cga
//
LOCUS       DASVOR010001678         3094 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_338345,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001678 DASVOR010000000
VERSION     DASVOR010001678.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338345"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="VF516_31345"
     CDS             complement(<1..799)
                     /locus_tag="VF516_31345"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEX8112286.1"
                     /translation="MTVAPRPPRPGALGAALSIALPGALAACLLLAVPGCLTTEYLLQ
                     AAHGQLDLLSKARPIDQVVRDPRTPIRVAAMLAEIPAIKDYGRSYGLTINRNYSTYAA
                     LGRPAAVWFVGAADPLAFKPLQWCFPIAGCFAGLGWFDEDDAVRHKLALDAQGYDTLI
                     RPASAYSTGGWFPDPVLSTMLGGGNDALPGLANVILHESVHATVLVPDEPFFNESFAS
                     YIADALTDHWIAVRFGPGSPEDLAWTLGQAVRLPFVARLLDAYQQLKK"
     gene            complement(796..1203)
                     /locus_tag="VF516_31350"
     CDS             complement(796..1203)
                     /locus_tag="VF516_31350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEX8112287.1"
                     /translation="MAETVFAKILRGEIPCHRIYEDDAVLAFLDVNPLSRGHTLVIPK
                     EPAETLDRLSDDAAAAVGRVLPRIARAVLAATGARAYNLLQNNGPEAHQAVFHVHFHI
                     IPKLDDGSGLGIGWKASKLVDGAELARAIAGRL"
     gene            complement(1245..1949)
                     /locus_tag="VF516_31355"
     CDS             complement(1245..1949)
                     /locus_tag="VF516_31355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8112288.1"
                     /translation="MIGRVLIVEDDDAIATGLALNLKLAGHANAIARDGVDALAQTEA
                     EDFGLILLDINLPRKNGLEVLAALRAADNLVPVIVLSAREHEFDKVAALRLGADDYVT
                     KPFALAELLARIDAVLRRTQQVIAAPVPVAAGHGDQLGFGDIAIEPGQRLVTRAGAEI
                     KLTHLEFELLVFFVRHPSQVFSRAQLFQLVWGQSAGSVRTVDNFVGQLRKRFEVNPEQ
                     PRHFVTVRGSGYRFDP"
     gene            complement(1946..2896)
                     /locus_tag="VF516_31360"
     CDS             complement(1946..2896)
                     /locus_tag="VF516_31360"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112289.1"
                     /translation="MRFPRANLQLRRAQVVLLLAVLLPTIAMIAVGVVLLATSAGSLP
                     AVISGVLVLTFCTSGITGYILGSLFLSKGAALVRVQNDFVASVSHELRTPLTSIHLLI
                     ESLRDGRLDAVERLQVLSLLARETGRLEALVGRVLELSRLQSSYDYARDPLDLAAVVD
                     EAIAAFDAITLTRPTPVILRLDPDLIVTGDRPTLVRALVNLLTNAWKYTGDDKQIELT
                     AIENRRWIEITVRDNGAGIEPAEQSRIFEQFQRGRAADASGATGLGLGLSFVTAIVRG
                     HRGKLDFESQPGETRFRIRLRRRRERVQVAGPALAERLAP"
     gene            complement(2905..>3094)
                     /locus_tag="VF516_31365"
     CDS             complement(2905..>3094)
                     /locus_tag="VF516_31365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112290.1"
                     /translation="VAARQGFFWWEVDATYYVLRGLAAVGLIWDLHGVPDHIRADARA
                     ESAPLGVLPAPAETADMA"
BASE COUNT          453 a         1065 c         1170 g          406 t
ORIGIN      
        1 ccttcttgag ctgctggtag gcgtcgagca ggcgggcgac gaacggcagc cggaccgcct
       61 ggccgagcgt ccacgccagg tcctccggcg agcccgggcc gaaccggacc gcgatccagt
      121 ggtcggtcag ggcgtcggcg atgtacgacg cgaagctctc gttgaagaac ggctcgtcgg
      181 ggaccagcac ggtggcgtgg accgactcgt ggaggatcac gttggcgagc ccgggcaggg
      241 cgtcgttgcc gccgcccagc atggtcgaca gcacggggtc ggggaaccag ccgccggtgg
      301 agtacgccga cgccggccgg atcagggtgt cgtagccctg ggcgtcgagc gcgagcttgt
      361 ggcgcacggc gtcgtcctcg tcgaaccagc ccaggccggc gaagcagccg gcgatcggga
      421 agcaccactg cagcggcttg aacgccagcg gatcggccgc gccgacgaac cacaccgcgg
      481 cgggccggcc gagcgcggcg taggtcgagt agttgcggtt gatcgtcagg ccgtagctgc
      541 ggccgtagtc cttgatcgcg gggatctcgg cgagcatcgc cgcgacccgg atcggcgtcc
      601 ggggatcgcg aacgacctgg tcgatcggcc gcgccttgct gagcaggtcg agctgaccgt
      661 gcgcagcctg cagcaggtac tcggtggtca ggcagccggg gacggcgagc aagaggcagg
      721 cggcgagcgc gccgggcagc gcgatcgaca gcgcggcgcc cagcgcgccg gggcgcggcg
      781 gacggggcgc gacggtcata gcctgccggc gatcgcccgg gcgagctccg cgccgtcgac
      841 cagcttgctc gccttccagc cgatgccgag gccgctgccg tcgtcgagct tggggatgat
      901 gtggaagtgg acgtggaaca cggcctggtg ggcctcgggg ccgttgttct gcaggaggtt
      961 gtaggcgcgc gctccggtcg ctgcgagcac ggcgcgcgcg atccgcggca ggacgcggcc
     1021 gaccgccgcc gcggcgtcgt cggacagccg gtccagggtc tcggccggct ccttggggat
     1081 caccagggtg tggccgcgcg acagcgggtt gacgtcgagg aacgcgagca cggcgtcgtc
     1141 ctcgtagatg cggtggcacg ggatctcgcc gcgcaggatc ttggcaaaca cggtctcggc
     1201 catggcgcag agcgtagcgt ggttgcggcc ggccggggcg cagcctacgg gtcgaagcgg
     1261 tagcccgagc cgcgcacggt gacgaagtgc cgcggctgct cggggttgac ctcgaaccgc
     1321 ttgcgcagct ggccgacgaa gttgtcgacc gtgcgcaccg agcccgccga ctggccccac
     1381 accagctgga acagctgggc gcgggagaac acctggctgg ggtggcggac gaagaacacc
     1441 aggagctcga actcgaggtg ggtgagcttg atttcggccc cggcgcgggt caccaggcgc
     1501 tggccgggct cgatcgcgat gtcgccgaac ccgagctgat cgccgtgccc ggccgcgacc
     1561 ggcaccggcg cggcgatgac ctgctgggtc cggcgcagca ccgcgtcgat ccgcgccagg
     1621 agctcggcga gcgcgaacgg cttggtcacg tagtcatcgg cgcccagccg cagcgcggcg
     1681 accttgtcga actcgtgctc gcgcgccgac agcacgatca ccgggaccag gttgtcggcg
     1741 gcgcgcagcg cggcgagcac ctcgagcccg ttcttcctgg gcaggttgat gtcgagcagg
     1801 atcagcccga agtcctcggc ctcggtctgg gccagcgcgt cgaccccgtc gcgcgcgatc
     1861 gcgttggcgt ggccagcgag cttcaagttg agcgccagcc cggtcgcgat cgcgtcgtca
     1921 tcctcgacga tcaggacccg gccgatcacg gcgccagccg ctccgccagg gccggcccgg
     1981 cgacctggac gcgctcgcgc cgccgccgga gccggatccg gaaccgggtc tcgccgggct
     2041 gcgactcgaa gtcgagcttg ccgcggtggc cgcgcacgat cgcggtcacg aacgacagcc
     2101 cgaggcccag gccggtggca ccgctggcgt cggcggcgcg gccgcgctgg aactgctcga
     2161 agatccggct ctgctcggcc ggctcgatcc cggcgccgtt gtcgcgcacc gtgatctcga
     2221 tccaccgccg gttctcgatc gcggtgagct cgatctgctt gtcatcgccc gtgtacttcc
     2281 acgcgttggt cagcaggttg accagcgcac ggaccagggt cggccggtcg ccggtcacga
     2341 tcaggtcggg gtcgagccgc aggatcaccg gggtcggccg ggtcagcgtg atcgcgtcga
     2401 acgcggcgat cgcctcgtcg accaccgccg ccaggtccag cggatcgcgc gcgtagtcgt
     2461 agctcgactg cagccgcgac agctcgagca cgcggccgac cagcgcctcg agccggccgg
     2521 tctcgcgcgc cagcagcgac aggacctgca gccgctcgac ggcgtcgagc cggccgtcgc
     2581 gcagcgactc gatcaggagg tggatcgacg tcagcggcgt gcgcagctcg tgcgacacgc
     2641 tggccacgaa gtcgttctgg acccggacca gcgcggcgcc cttggacagg aacaggctgc
     2701 ccaggatgta gccggtgatg ccgctggtgc agaacgtcag gaccagcacg cccgagatca
     2761 cggcgggcag gctgcctgcg ctggtcgcca gcagcacgac gccgaccgcg atcatcgcga
     2821 tcgtcggcag cagcacggcg agcaacagca ccacctgtgc gcggcggagc tgaaggttcg
     2881 cccgcgggaa ccgcatcgcg acgactatgc catgtcggcg gtctcggccg gcgccggcag
     2941 cacgccgagc ggcgcagatt ctgcgcgggc gtccgcgcgg atgtggtcgg ggacgccgtg
     3001 gaggtcccag atcaggccga ccgccgcgag gccgcggagc acgtagtacg tggcgtcgac
     3061 ctcccaccag aagaagccct ggcgcgccgc cacc
//
LOCUS       DASVOR010001679         1788 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_484971,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001679 DASVOR010000000
VERSION     DASVOR010001679.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_484971"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(284..1150)
                     /locus_tag="VF516_31370"
     CDS             complement(284..1150)
                     /locus_tag="VF516_31370"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8112291.1"
                     /translation="MRFSINIPNFGDFADPRTVATVAAAAEEAGWDGLFVWDHVVHDK
                     AKRRGQPFADPWMLLTAAALATSRLRLGTMVTPVARRRPEQLARQVATLDVLSGGRVI
                     FCAGLGGPIEDEFGSFGDTTDPVVLAERLDEGLELMGRYWSGEQVNHQGSHYRVRDVT
                     LLPAPVQRPRPPVWIGGFWPHRRPMRRAARWDGAVPLFTAAGHGQVPPLEEVRELVAY
                     IGRHREGRAEPFELVLGGASPAGPAEARETIAALAEAGATWWDERQLQDSDDLYRHAP
                     VLRRIDQGPPSF"
     gene            complement(1206..1278)
                     /locus_tag="VF516_31375"
     tRNA            complement(1206..1278)
                     /locus_tag="VF516_31375"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1243..1245),aa:Arg,seq:gcg)
     gene            1324..>1788
                     /locus_tag="VF516_31380"
     CDS             1324..>1788
                     /locus_tag="VF516_31380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112292.1"
                     /translation="MTIALQVIVSGLAAGGVYGLFAAGHSLIHRLTGIVHFAFGDLVG
                     LGIFATLLVLAGTAPVSQSGAHEPRFLFALIVGLGVCVLAGVGTYVGLVQPYLSRGST
                     IGWVAGTAGFVHLGGAQVQARAFFVAGLAIALALGSTWFLERTRRGRALRAVA"
BASE COUNT          222 a          654 c          647 g          265 t
ORIGIN      
        1 gtcgacgagg cgagccccgg cgacgagatc gtcctcggcg acaagacggt gggccggatc
       61 acgagcgcgg tccccggccg cgctctcggc tacgtcaggc gcgaggtgcc cgacgacgcg
      121 gcgctgcgcg tcggctccac cgaggcccgg ctgatcgcct agccgccggc aagctcgcgg
      181 cggcggcgct cgagccgacg cagcttcgac ttgagctcgc gcgcaaaggc gtcgagcgtc
      241 tcctgccgtg acgagctcga gctggcgctg gacgacgcgc gctctagaag gaaggcggcc
      301 cctggtcgat gcgccgcagg accggagcgt gccggtagag gtcgtcgctg tcctggagct
      361 gccgctcgtc ccaccaggtg gcgccggcct cggccagcgc cgcgatcgtt tcccgcgcct
      421 cagcggggcc cgcggggctg gcgccgccga ggacgagctc gaacggctcg gctcgcccct
      481 cgcggtgcct tccgatgtac gcgaccagct cccggacctc ctcgagcggc gggacctgcc
      541 cgtgcccggc ggcggtgaac agcggaacgg cgccgtccca gcgcgctgcc cggcgcatcg
      601 gccggcggtg tggccagaag ccgccgatcc agaccggcgg ccgcgggcgc tgcaccggcg
      661 cgggaagcaa ggtcacgtcc cgcaccctgt agtggctccc ctggtggttc acctgctcgc
      721 ccgaccagta gcgccccatg agctccaggc cttcgtcgag gcgctcggcc agcacgaccg
      781 ggtcggtggt gtccccgaag ctgccgaact cgtcctcgat cggcccgccg aggccggcgc
      841 agaagatcac cctgccaccg ctgaggacgt ccaacgtcgc gacctggcgc gcgagctgct
      901 cgggccttcg ccgggctacc ggtgtgacca tcgtccccag cctgagccgg gaggtcgcca
      961 gcgccgccgc ggtcaagagc atccacggat cggcgaacgg ctgtcctcgc cgcttcgcct
     1021 tgtcgtggac gacgtgatcc cagacgaaga gcccgtccca gcccgcctcc tcggccgcag
     1081 cggcgaccgt cgccacggtc ctgggatcgg cgaagtcgcc gaagttcgga atgttgatcg
     1141 aaaatcgcat ccggagtgcg ccgttccggc tgcagcgact cgtccgagac ggcttggtcg
     1201 cgccatgcgc ccggcatgat tcgaacatgc gacctctgcc tccgcagggc agcgctctat
     1261 cccctgagct acgggcgcgc gaagggggag tgtagccgcg gcctgtagta caagcttccg
     1321 cgcgtgacga tcgcgctgca ggtcatcgtc tcgggtctcg ccgcaggagg ggtctacggt
     1381 ctcttcgccg ccgggcactc tctgattcac cggctgacgg ggatcgtcca cttcgccttc
     1441 ggcgacctgg tcggcctagg gatcttcgcg acgctgctgg tgctcgcggg gacggcgccg
     1501 gtgagccaga gcggcgcgca cgagccgagg ttcctgttcg cgctgatcgt ggggctcggc
     1561 gtctgcgtgc tggcgggcgt cggcacctac gtcgggctcg tccagccgta cctgagccgc
     1621 ggctcgacga tcggctgggt cgccgggacg gccggcttcg tgcacctcgg cggggcgcag
     1681 gtgcaggcgc gggcgttctt cgtcgccggg ctcgcgatcg cgctcgcgct cgggtcgacg
     1741 tggttcctcg agcgcacgcg gcgcggccgc gcgctccgcg ccgtcgcc
//
LOCUS       DASVOR010001680         7860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_338348,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001680 DASVOR010000000
VERSION     DASVOR010001680.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7860
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338348"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="VF516_31385"
     CDS             complement(<1..1581)
                     /locus_tag="VF516_31385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8112293.1"
                     /translation="MAEKIVAPMVQQRAANKPEPSDEATVHAAASRGVATPASSLPFS
                     DTLQRVFGRHDISVIQAHTGLEAAESAQAMGAEAYATGDHVVLGRGADLHTVAHEAAH
                     VVQQRRGVQLKGGVGTVEDAYERHADAVADRVVTGKSAEDLLDQGAASVAPGATLAVQ
                     RKEGKDDTKILDNQASLKGADVEIPALEGALLATRKEAVKLGLLSQVSFDAGLALSQA
                     MTQLQPAVAAKGAVDPGVQERAAVAAQQLFVALQRETTGDKNFQIVPSMTPSGGVTLQ
                     NPYTEEVKVTTTFLFWSSTHEVGSWLQELPGLIRQGKWDEAFRGYRRMLDGLDLWVPD
                     QLRRKGKGTPEEALGNAQQHHAQLRTGLEQIADKHATRLPALFHPDPKTVEKEKVAGR
                     PAADTIPMNVYFWKDATDGKFHIYDLTTPSRPHEQTIDGQPTAAMMNTFFEEVARYPE
                     GEVRYTLPGGTAGTAPTTGKTKWSEWGGYAGLAIAAVGLALLSAGASIPATVCFAAGA
                     VAGGVSSGGHLVDTAHLGT"
     gene            3189..5243
                     /locus_tag="VF516_31390"
     CDS             3189..5243
                     /locus_tag="VF516_31390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112294.1"
                     /translation="MNRRLSMQRSALGAVVVALAAAGAGCGNDRAGGDDGGGTTPFVA
                     DPPSVYVAKVKNLLVGLPPTGDEVAQVTADPSKLGGLIDGWMQLPQYQQKMMVFFELA
                     FQQTQITATDFVDIVPTQGLGAGRMIPLLVQNTRESFARTVLELTKQGRPLTDAFTTH
                     QLMMTPALMELYAFLDTRQVNDAAQVNDLFAKANTGLKITMETSAGPIPITDSVNPAS
                     PNFMHWYTPDLPTLTYPDTTCNGLDPITFNVNAQALHALLYGEIPNHPGPSGNCGNRA
                     GSVMSAQVAATDFTAWKMVTIRAPKGTEARTVFYDLPALRTATELVLSTPRVGFFSTP
                     AFFANWPTNSSNQMRVTVNQALIVATGAAIDGTDSTSPPTTPGLDAQHAAPNTACYGC
                     HQQLDPTRSILSSTYSWFYYPQTDTTLIKQPGLFAFQNVIAPMQTIDDFAHLLATHPL
                     VGQAWAQKLCYYANSAPCNPIDPEFLRIVEKFTSAGGTWNTLVRELMASPITTNTAET
                     ATSKTNGEVVAVSRRDHLCAALDNRLGFVNICELDSTLGGRAPGQAAIAQIVSGMPSD
                     GYGRGSTIPVLPNDPTLFYRAGLENVCAQVAAMTIDAKANANQPGAKAWSSAQPDAAI
                     AEFVSTVMALTPSDPRAAQASSILTSHFHAAMQAGQTASDSLKSTFVAACLSPSFIGI
                     GM"
     gene            5255..6568
                     /locus_tag="VF516_31395"
     CDS             5255..6568
                     /locus_tag="VF516_31395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112295.1"
                     /translation="METSRRKVLMSTLFGAGYIGLRALATGIPASVLLQGRRAFADGA
                     PACTDKSKAQFIVLATSGSGDPINANVPGTFEDTRITHSADPTMKPASMTLGGKTVTA
                     ALPWTQLPQSVLDRTAFWHIMTNTPVHPKEPDVLKLMGMTQYDEMLPSILAKQLAPCL
                     GTVQNQPITLGATSPSEGLSFGGQALPIIPPLALKATLTSPAGPLANLQKLRDQTLDQ
                     INDLFRGGASPAQKAYIDSLVASQGQIRNIRQDLLSALTSIKDNSVASQITAAVTLIQ
                     MNVTPVIAIHIPFGGDNHTDAALANEAAQHVSGVAAIGSLMTQLASASLSDQVTFMSL
                     NVFGRTLGPSNTNGRNHNPNHQVSITIGKPFKPGVIGGVVPVASDYGAMAIDSKTGVG
                     VAGGDIDAGSTLASFGKTALAAVGVDSATIDTLISTGKVVPGALA"
     gene            complement(6581..7666)
                     /locus_tag="VF516_31400"
     CDS             complement(6581..7666)
                     /locus_tag="VF516_31400"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112296.1"
                     /translation="MQNAYRADQVGSLLRPAELVDARHHDAAPDAVRALEDRHILRLL
                     ERQRALGLDVITDGELRRSNFMSDLWDAVDGFDFGDAVARSWSGAKVSSVTGVVNRKL
                     VQRRGLTAPELPFLQQHAAGAPIKMTLPSATQFPAIAFKRGITDKVYPTPSDLLWAIA
                     DIIQAEMRTLAEAGIAYLQIDAPRYSYYIDPKWRAWLRDEFGVDPDQSLDEAVRVDNA
                     CFDAARRAGVTVAIHLCRGNNRSHWYAEGGYDAIAEKLFGELRVDRFLLEYDDQRSGG
                     FEPLRFVPRGKIVVLGLVSSKLAQLESADTLRRRIDEASKFVPLDELALSPQCGFAST
                     MEGNLLTEDDQWAKLRLIVETARAVWR"
BASE COUNT         1236 a         2658 c         2736 g         1230 t
ORIGIN      
        1 cgtgccaaga tgggccgtgt cgacgaggtg gccgccggat gaaacgccgc cggccaccgc
       61 ccccgccgcg aagcacaccg tcgcggggat gctggcgcca gcgctgagca acgccaggcc
      121 gacggcggcg atcgccagcc ccgcgtaacc cccccactcg gaccacttgg tcttgccggt
      181 ggtgggcgcg gtgcctgcag tgccgcccgg cagtgtgtag cgcacctcgc cctcggggta
      241 ccgcgcgacc tcctcgaaga acgtgttcat catcgccgcg gtcggctggc cgtcgatggt
      301 ctgctcgtgc ggccggctcg gcgtcgtgag gtcgtagatg tggaacttgc cgtccgtggc
      361 gtccttccag aagtagacgt tcatcgggat ggtgtcggcg gcgggccggc cggccacctt
      421 ctccttctcg acggtcttgg gatcggggtg gaacagggca ggcagccgcg ttgcatgctt
      481 gtccgcgatc tgctcgagcc cggtgcggag ctgcgcatgg tgctgctgcg cgttgccgag
      541 cgcctcctcg ggcgtgccct tgcccttccg gcgcagctga tcgggcaccc acaggtcgag
      601 cccgtcgagc atgcggcggt agccgcggaa cgcctcgtcc catttgccct ggcggatgag
      661 ccctggcagc tcctggagcc agctcccgac ctcatgggtg ctcgaccaga acaggaaggt
      721 ggtggtgacc ttcacctcct cggtgtacgg attttgcaag gtgacgccgc cgctcggcgt
      781 catcgacggg acgatctgaa agttcttgtc gccggtggtc tcgcgttgca gcgcgacgaa
      841 cagctgctgc gctgcgaccg cggcgcgctc ctgaacgccc ggatcgacgg cgcccttcgc
      901 ggccacggcc ggctggagct gcgtcattgc ctgggacagc gccagcccgg catcgaacga
      961 tacttgcgac agcagaccca acttgaccgc ctccttccgg gtggccagca gggcgccctc
     1021 gagcgcgggg atctcgacgt cggctccttt gaggctggcc tgattgtcga ggatcttggt
     1081 gtcgtccttg ccctccttgc gctggaccgc cagcgtcgct cccggcgcaa cgctcgccgc
     1141 gccttgatcc aggaggtcct cggccgactt gccggtgacc acccggtccg cgaccgcatc
     1201 ggcgtggcgc tcgtaggcgt cctccacggt accgacgcca cccttcagct ggacgccgcg
     1261 gcgctgctga acgacgtgcg cggcttcgtg cgcaacggtg tgcagatcgg cgccgcggcc
     1321 caggacgaca tgatcgcctg tcgcatacgc ctcggcgccc atggcctgcg cggactcggc
     1381 ggcctcgagg ccggtgtggg cctggatcac cgaaatgtcg tggcgcccga acacgcgctg
     1441 gagcgtgtcc gagaacggca gggacgaggc gggcgtcgcc acaccccgtg acgccgcagc
     1501 atgcacagtg gcctcgtcac tcggctcggg cttgttggcg gcgcgttgct gcaccatcgg
     1561 cgcgacgatc ttctcggcca aggtccgctt gcccggatct gactgctctg cgtgcacttc
     1621 cggtcgagat cccagggacc cgtggcaccc tcgcgacttg cctgcgtagc tcaccgcctg
     1681 atgcccttcc gctgctacgg tacccgatcc cacggcgcgc atcggtactg cttcgcggaa
     1741 tcacgcctca cgtatgcacg gagcggcgat cgatggaagg tgtacgtcag gctgagagcg
     1801 agaccgatgg ctccgttagt tgcgtcggcc agacaacggt ttctgcgtgt ctggaggagc
     1861 gctacagctg tccccgcatg ggttgaagca gttggcggac ggtgataaaa gccgtcgcgt
     1921 gaccgggctg ccacgggttt cgagcgtgat cgcatgcctg ctgtggctga gcgcttgcgg
     1981 tttcccacgg ccggcggatg tcgagagcga tggcggcggg gtggatgctg ccgcaacgag
     2041 cggttcgctg gatgcggcga cgtgtttcgg gagcttggtg aagatctgcc tcgcgtcaac
     2101 accgaccgtg ccctcacggt ctcgacgcca tcgaacatcg acactgacag cccactatgc
     2161 gcgagcacgg tgagtggggc aacgaactac tgtgtgatcg cagcaacgaa tatcacgatc
     2221 ggggcaacgt tgcgtgcgac agggacgaag ccgttggttt tgattgcaac cgattctatc
     2281 atgactaccg cgcttcttga tgtcggaagc caccgcgatg tgaccgcaag tctgctcggc
     2341 cacgcagcca tgagcttcct gggccctgcc atcggcgcgg gcgctaatcc gagcagctgt
     2401 gccgacccga tgccaccgat ggacggtggc ggatatgctg ggggtagctt catcggcttc
     2461 ggcggtgtgg gaggtcttgg cagcaacgga agtaccggcg gctcacccgc tgaggcggtg
     2521 acagaggttt ctgagttgcg tggcggctgc tcctcattcc caaactcgcc tttcagaaga
     2581 ggcggctacg gcggcggtgc cgtatatctc atcgcgggaa agcgcataga cgtacaaggt
     2641 ggcatcaatg catccggcgc aggtggcata ggcagtaccc aggggaacct tgggggagtt
     2701 ggaggtgggg caggtggaat gatcggcttt gaagcgcccg agatcacctg taacagcctt
     2761 ctccttgcaa gcggcggcgg cggcggtggg ggaagttcgg gtataagtcc gggcggcgac
     2821 ggccaggatc caagcagtct ggcagcggca gctggagggg gtggcgcaaa cggaggtagc
     2881 ggtggcagcg gctcgacagc gtcatcgcgc gccgccccca gcacccctgg cggcccagga
     2941 agttcagcgg gtatcggcgc tggcggtggt ggcggcggcg gcggcgccgg cctgatcaag
     3001 gcgccggcca ccgcgaacct cggcaccatg gtgtccccgc cgcccacacc ctgaccctgt
     3061 agggtcgacc ctgcagctgc tgtggggtca ggccgacaag gggtcggacg atcgcctccg
     3121 ggtggccgga ttgtcgcgca attccagggg ggtatcgctg gcctgcggct tgctcgatgg
     3181 gattggccat gaaccggaga ctctcgatgc aacgctcggc gctgggcgcc gtggtcgtgg
     3241 ccctggccgc cgccggggcc ggttgcggca atgaccgggc agggggcgat gacggcggcg
     3301 gcaccacgcc gttcgtcgcg gacccgccgt cggtctacgt cgcgaaggtc aagaacctgc
     3361 tggtcgggct gccgccgacc ggtgacgagg tcgcgcaggt gacggccgat ccgagcaagc
     3421 tcggcgggct gatcgacggc tggatgcagc tgccgcagta ccagcagaag atgatggtgt
     3481 tcttcgagct ggcgttccag cagacgcaga tcaccgcgac ggatttcgtc gacatcgtgc
     3541 cgacccaggg gctcggcgcg ggccgcatga tcccgctgct ggtgcagaac acgcgcgaga
     3601 gcttcgcgcg cacggtgctc gagctgacca agcagggccg gccgctgacc gacgcgttca
     3661 cgacccatca gctgatgatg acgccggcgc tgatggagct gtacgcgttc ctcgacacgc
     3721 ggcaggtcaa cgacgcggcg caggtcaacg acctgttcgc gaaggccaac accgggctga
     3781 agatcacgat ggagacgtcg gccgggccga tcccgatcac cgacagcgtc aacccggcga
     3841 gcccgaactt catgcactgg tacacgccgg acctgccgac gctgacgtac cccgatacga
     3901 cgtgcaacgg gctcgacccg atcacgttca acgtcaacgc gcaggcgctg cacgcgctgc
     3961 tgtacggcga gatcccgaac caccccgggc cgtcgggcaa ctgcggcaac cgcgccggct
     4021 cggtgatgag cgcgcaggtc gcggcgaccg acttcacggc ctggaagatg gtgacgatcc
     4081 gggcgcccaa gggcaccgag gcgcgcacgg tgttctacga cctgccggcg ctgcgcaccg
     4141 cgaccgagct ggtgctgtcg acgccgcggg tcgggttctt ctcgacgccg gcgttcttcg
     4201 cgaactggcc gaccaactcg agcaaccaga tgcgggtcac ggtcaaccag gcgctgatcg
     4261 tggcgaccgg cgcggcgatc gacgggacgg attcgacgtc gccgccgacc acgcccgggc
     4321 tcgacgcgca gcacgcggcg ccgaacacgg cgtgctacgg ctgccaccag cagctcgatc
     4381 cgacgcgctc gatcctgtcg agcacgtact cgtggttcta ctacccgcag accgacacga
     4441 ccctgatcaa gcagcccggg ctgttcgcgt tccagaacgt gatcgcgccg atgcagacga
     4501 tcgacgactt cgcgcacctg ctggcgaccc acccgctggt cggccaggcc tgggcccaga
     4561 agctgtgcta ctacgccaac tcggcgccgt gtaacccgat cgatcccgag ttcctgcgga
     4621 tcgtcgagaa gttcacgtcg gcgggcggga cctggaacac gctggtccgc gagctgatgg
     4681 cgtcgccgat cacgaccaac acggccgaga ccgcgaccag caagaccaac ggcgaggtcg
     4741 tcgcggtgag ccggcgcgat cacctgtgcg ccgcgctcga caaccggctc ggcttcgtca
     4801 acatctgcga gctcgattcg acgctgggcg gccgtgcgcc ggggcaggcg gcgatcgcgc
     4861 agatcgtcag cggcatgccg tcggacggct acggccgcgg ctcgacgatc ccggtcctgc
     4921 ccaacgaccc gacgctgttc taccgcgccg gactggagaa cgtgtgcgcg caggtcgcgg
     4981 cgatgacgat cgacgccaag gccaacgcga accagccggg ggccaaggcg tggtcgagtg
     5041 cgcagccgga cgcggcgatc gccgagttcg tgtccacggt catggcgctg acgccgtccg
     5101 atccgcgggc ggcgcaggcc agctcgatcc tcacgtcgca cttccacgcc gcgatgcagg
     5161 cgggccagac cgcctcggac tcgctgaagt ccacgttcgt cgccgcgtgt ctgtccccgt
     5221 cgttcatcgg catcgggatg taaggagagc cgccatggaa acctcgcgtc gcaaagtgct
     5281 catgtccacg ctgttcggag ccggttacat cgggctccgc gcgctcgcca ccggcatccc
     5341 cgcctccgtg ctgctccagg gtcgtcgcgc gttcgccgac ggcgcgccgg cctgcaccga
     5401 caagtcgaag gcgcagttca tcgtgctcgc cacctcgggc agcggcgatc cgatcaacgc
     5461 caacgtcccc ggcacgttcg aggacacccg gatcacgcac agcgccgatc cgacgatgaa
     5521 gccggcgtcg atgacgctcg gcggcaagac ggtgaccgcg gcgctgccgt ggacccagct
     5581 gccgcagagc gtgctcgacc ggacggcgtt ctggcacatc atgaccaaca cgccggtgca
     5641 ccccaaggag cccgacgtgc tcaagctgat ggggatgacc cagtacgacg agatgctgcc
     5701 gtcgatcctg gccaagcagc tcgcgccgtg cctgggcacg gtgcagaacc agccgatcac
     5761 gctgggcgcg acgtcgccga gcgagggcct gagcttcggc ggccaggcgc tgccgatcat
     5821 cccgccgctc gcgctcaagg cgacgctgac cagcccggcc ggcccgctcg ccaacctcca
     5881 gaagctgcgc gatcagacgc tcgaccagat caacgacctg ttccgcggcg gggcgagccc
     5941 ggcgcagaag gcgtacatcg actcgctggt cgcgtcgcag ggccagatcc gcaacatccg
     6001 gcaggacctg ctgagcgcgc tgacgtcgat caaggacaac agcgtggcgt cgcagatcac
     6061 cgcggcggtc acgctgatcc agatgaacgt cacgccggtg atcgcgatcc acatcccgtt
     6121 cggcggcgac aaccacaccg acgcggcgct cgccaacgag gcggcgcagc acgtgtcggg
     6181 ggtcgcggcg atcggctcgc tgatgacgca gctggcgtcg gcctcgctgt ccgatcaggt
     6241 cacgttcatg tcgctcaacg tgttcggccg cacgctcggg ccgagcaaca ccaacggccg
     6301 caaccacaac ccgaaccacc aggtgtcgat cacgatcggc aagccgttca agcccggcgt
     6361 gatcggcggg gtcgtcccgg tcgccagcga ctacggcgcg atggcgatcg actcgaagac
     6421 cggggtgggc gtcgccggcg gcgacatcga cgccggctcg acgctggcat cgttcggcaa
     6481 gaccgcgctg gccgcggtcg gcgtcgactc ggcgacgatc gacaccctga tcagcaccgg
     6541 caaggtggtg ccgggcgcgc tggcctgagc cgcgggcggc tcaccgccac acggcgcgcg
     6601 cggtctcgac gatcaggcgc agcttggccc actgatcgtc ctcggtcagc aggttgccct
     6661 ccatggtcga cgcgaagccg cactgcgggc tgagcgcgag ctcgtcgagc ggcacgaact
     6721 tgctggcctc gtcgatccgg cggcgcaggg tgtcggccga ctcgagctgg gcgagcttgc
     6781 tgctgaccag gccgagcacg acgatcttgc cgcgcggcac gaaccgcagc ggctcgaacc
     6841 cgccggagcg ctggtcgtca tactccagca ggaagcggtc gacccggagc tcgccgaaca
     6901 gcttctcggc gatcgcgtcg tagccgccct cggcgtacca gtggctccgg ttgttgccgc
     6961 ggcacaggtg gatcgcgacg gtgacgccgg cccggcgcgc ggcgtcgaag caagcgttgt
     7021 cgacccgcac cgcctcgtcg agcgactggt cggggtcgac gccgaactcg tcgcgcagcc
     7081 acgcgcgcca cttcggatcg atgtagtagc tgtagcgcgg ggcgtcgatc tggaggtacg
     7141 cgatgccggc ctcggcgagc gtgcgcatct cggcctggat gatgtcggcg atcgcccaca
     7201 gcaggtccga cggcgtcggg tagaccttgt cggtgatgcc gcgcttgaag gcgatcgcgg
     7261 ggaactgggt cgcgctcggc agcgtcatct tgatcggcgc gccggcggcg tgctgctgga
     7321 ggaacggcag ctcgggggcg gtcagcccgc ggcgctggac cagcttgcgg ttgacgacgc
     7381 cggtcacgct cgacaccttg gcgccgctcc agctgcgcgc caccgcgtcg ccgaaatcga
     7441 acccgtcgac cgcgtcccac agatcgctca tgaagttgct gcgccggagc tcgccgtcgg
     7501 tgatcacgtc gagcccgagc gcgcgctggc gctcgagcag ccgcaggatg tggcgatctt
     7561 cgagcgcgcg caccgcgtcg ggtgcggcgt cgtggtggcg ggcatcgacg agctcggcgg
     7621 gcctcagcag gctgccgacc tggtccgcgc gataggcgtt ctgcatggcg gaaacatacg
     7681 cctgcgcggc agcggcacga agcggtttgc gcctgagctg gcatccgtat gcggctgtgt
     7741 gatgatgcgc gcgaggaccc acccgatggc cgaatccatg cgctggcgac tcaggttttc
     7801 gagggggtcg cgatgggctc gctgatcgcc gcgcgcatgg catcgaccac cgagatcctg
//
LOCUS       DASVOR010001681         3060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_221916,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001681 DASVOR010000000
VERSION     DASVOR010001681.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221916"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..801)
                     /locus_tag="VF516_31405"
     CDS             complement(280..801)
                     /locus_tag="VF516_31405"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX8112297.1"
                     /translation="MSEKNGLPTGKHTLVEEGTEFKGTMSSSCPIVVMGKVEGDVAGP
                     VIHVTPSGVVSGVVKVKQLRSDGELAGEVEADTVEISGRVRDRTVIRARSLEVSLSVQ
                     KGGMQVVFGECELAVGDEPSKEAAVASALTPPAPAESAKQPPAAAKPANSGPDDAAKR
                     RRTPGTEPPPSPT"
     gene            complement(824..2074)
                     /locus_tag="VF516_31410"
     CDS             complement(824..2074)
                     /locus_tag="VF516_31410"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8112298.1"
                     /translation="MGLSRDEALKRIQRGDKLDRADLKGIDLSQAKLARAALDRADLD
                     GANLEGTDLSSAALRRASLREAFLVGANLSGANLENADLEGAKLERAILVGANLTRAN
                     LEGAVLTGANLSGALVSYAQLTGAKLGGANLADAVFTHAELDEAYLGGASAPSARFSN
                     ASLAGANLEDAVMRGANLDETVLHRAIARNTDFTGGSLVKTNFSGADLTGAKLGGADV
                     RHATFARAQLAGAHLTGAKVAGLVGTGQPIGTIDVGWLDLSPAGDGTQRVENGVIPSV
                     LTVGAVQVVAEDARRRYFGRGDVLRNATLQFDAGAKVEIDSLFQNCTINVGADTELVI
                     GPSGVLADCEIIGAGQITVHGKFFEKASPGIVGARKLMVSSRGAVVSTVAQSGEPTGF
                     AFQSGCQLRVKIIPPGKAPASGTR"
     gene            complement(2106..>3060)
                     /locus_tag="VF516_31415"
     CDS             complement(2106..>3060)
                     /locus_tag="VF516_31415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112299.1"
                     /translation="DSVGAAFDQVIEADVSDTAVMAAQSVHGVSTVEDLAEVRRVFND
                     VAAIHVAQVRDVMLELRYGDAQPGWIESTQPALRSLRAMAQQMDLHDLVEALDGFCVA
                     VDAAVTGRARIDDDGKAELLRRYQRLIELIPQAFELDAERDRREPIIVEALLYQIPGV
                     ERRTVEKLFTVGLHRLDPLIHASADEVAVVAGIRRELATAIVDQFRTYLSAASSALAA
                     PDPIAERRALGDLLIMMSVQNDEYNHAASLWTDEARGRKQEARKQREHVFQRIKVALA
                     RLGERDLLARLDKLPFNDRIAALDRYLSAAIPQKPQPGKTG"
BASE COUNT          380 a         1054 c         1146 g          480 t
ORIGIN      
        1 ccgcgagcag gatggggtcg gacgccgcgg ccgtgatttg gattggccgg cgttgttccg
       61 cacatacctc gcgcgccgcc cgcgcatgca tcggtggtga ttcggcggac gcggctccga
      121 cacccatccg cgcagcagcc cacggcgaga tcgcgtgtcg gcgttctcct gggcgccacg
      181 cccatcgtca ccgtcgccgt cgcgcgggca cgccgaggtc gaccgcaacg tcttcgcgag
      241 gacgttccgg ccgttcagcg acgcggcttg gccagccgat caggtcggcg acggcggcgg
      301 ctcggtgccg ggggtgcggc ggcgcttggc ggcgtcgtcg gggccgctgt tggcgggctt
      361 ggcggcggcg ggcggctgct tggcggactc ggccggggcc ggcggggtca gcgccgacgc
      421 gaccgcggct tccttgctgg gctcgtcgcc gaccgcgagc tcgcactcgc cgaacacgac
      481 ctgcatgccg cccttctgga cgctgagcga gacctccagc gagcgcgcgc ggatcacggt
      541 gcgatcgcgg acgcggccgg agatctcgac cgtgtcggcc tcgacctcgc cggcgagctc
      601 gccgtcggac cgcagctgct tgaccttgac gacgcccgac acgacgccgc tcggggtgac
      661 gtggatgacc ggaccggcga cgtcgccctc gaccttgccc atcacgacga tcgggcagct
      721 ggaggacatc gtgcctttga actcggtccc ctcttcgacg agggtatgct tgccggtggg
      781 aagtccgttc ttctcgctca tgtcgcctcg ttcgtggggt tggtcacctg gttccggacg
      841 ccggggcctt gcccggcggg atgatcttga cgcgcagctg acagccgctc tggaacgcga
      901 acccggtcgg ctcgccgctc tgcgccacgg tcgacaccac ggcgccgcgc gagctgacca
      961 tcagcttgcg cgcgccgacg atgccggggc tggccttctc gaagaacttg ccgtggacgg
     1021 tgatctggcc ggcgccgatg atctcgcagt cggcgagcac gccggacggg ccgatcacca
     1081 gctcggtgtc ggcgccgacg ttgatcgtgc agttctggaa caggctgtcg atctcgacct
     1141 tggcgccggc gtcgaactgc agggtcgcgt tgcgcagcac gtcgccgcgg ccgaagtagc
     1201 ggcggcgcgc atcctcggcg acgacctgga ccgcaccgac ggtgagcacc gacgggatca
     1261 cgccgttctc gacccgctgg gtgccgtcgc cggccgggct gaggtcgagc cagccgacgt
     1321 cgatcgtgcc gatcggctgg ccggtgccga ccaaacccgc gaccttggcg ccggtcaggt
     1381 gagcaccggc gagctgggcg cgcgcgaacg tcgcgtggcg gacgtcggcg ccgccgagct
     1441 tggcgccggt caggtcggcg ccggagaagt tggtcttgac cagcgagccg ccggtgaagt
     1501 cggtgttgcg cgcgatcgcg cggtgcagca cggtctcgtc gaggttcgcg ccgcgcatca
     1561 ccgcgtcctc gaggttggcg ccggccaggc tggcgttgct gaaccgcgcc gacggcgccg
     1621 acgcgccgcc gaggtaggcc tcgtcgagct cggcgtgggt gaacaccgcg tcggcgaggt
     1681 tggcgccgcc gagcttggcg ccggtgagct gggcgtagct gaccagcgcg cccgacaggt
     1741 tggcgccggt cagcaccgcg ccctcgaggt tggcgcgggt caggttggcc ccgaccagga
     1801 tcgcgcgctc gagcttggcg ccctcgaggt cggcgttctc gaggttggcg ccgctcaggt
     1861 tggcgccgac caggaaggcc tcgcgcaggc tggcgcggcg cagcgcggcg ctcgacaggt
     1921 cggtgccctc gaggttggcg ccgtcgaggt cggcccggtc gagcgccgcc ctggcgagct
     1981 tcgcctgcga cagatcgatg cccttgagat cggcgcggtc gagcttgtcg ccgcgctgga
     2041 tccgcttgag tgcctcgtcg cgtgataggc ccatcgtcgt tgctccggtt gctccgtcgt
     2101 tgcgatcagc cggtcttgcc cggctgcggt ttctgcggga tcgccgcgga caggtaacga
     2161 tcgagtgcgg cgatccggtc gttgaacggc agcttgtcga gccgcgccag cagatcgcgc
     2221 tcgccgagcc gggcgagggc gaccttgatg cgctggaaca cgtgctcgcg ctgcttgcgg
     2281 gcctcctgct tgcggccgcg cgcctcgtcg gtccacagcg acgccgcgtg gttgtactcg
     2341 tcgttctgca cgctcatcat gatcagcaga tcgccgagcg cgcggcgctc ggcgatcgga
     2401 tcgggcgcgg cgagcgccga gctggcggcg ctgaggtagg tccggaactg atcgacgatc
     2461 gcggtcgcga gctcgcgacg gatcccggcg accacggcga cctcgtcggc ggaggcgtgg
     2521 atcagcgggt cgagccggtg caggccgacc gtgaacagct tctcgaccgt gcgccgctcg
     2581 acgccgggga tctggtagag cagcgcctcg acgatgatcg gctcgcggcg gtcgcgctcg
     2641 gcgtcgagct cgaacgcctg cgggatcagc tcgatcagcc gctggtagcg gcgcagcagc
     2701 tcggccttgc cgtcgtcgtc gatccgggcg cggccggtga ccgcggcgtc gaccgcgacg
     2761 cagaatccgt cgagcgcctc gaccaggtcg tggaggtcca tctgctgggc catcgcgcgc
     2821 agcgagcgca gcgcgggctg ggtcgactcg atccagccgg gctgggcgtc gccgtagcgc
     2881 agctcgagca tgacgtcgcg gacctgggcg acgtggatcg ccgcgacgtc gttgaacacg
     2941 cggcggacct cggcgaggtc ctcgaccgtc gacacgccgt ggaccgactg ggcggccatc
     3001 accgcggtat cgctgacatc ggcctcgatc acctgatcga acgccgcacc gaccgagtcg
//
LOCUS       DASVOR010001682         6423 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_396500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001682 DASVOR010000000
VERSION     DASVOR010001682.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6423
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1095
                     /locus_tag="VF516_31420"
     CDS             <1..1095
                     /locus_tag="VF516_31420"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8112300.1"
                     /translation="AYVGAADATWKSSDGSVELDAMAGGSVLDAARGNGATGYARANK
                     RWDGGWYASATTDYASRRFNPNDLGYLEASDHVELLGEAGRQWDRTLGVFRNWSIAAT
                     ANYGQDHAGRAFNDNAGVRIATGFTNDWTAYAGAADSFPHHDDRELRAFLDPVKKYLH
                     HGHEPNAIAGFTSTHRSAWYAGLDLSVTHYAGGPTYTATFNQTIHPSSRFELELVTDL
                     AVARGEQHWLETQGDTPIVGLRRLVQLSQIVRASYAFTPNLTLQAYTQLLLATWHYRD
                     LARYVDDNTLAPGAMAGTTSFSASNFNVNGVLRWEIRPGSTLYAVYTHGAFDGEVFHR
                     DATLSSRSVSALLRAPSDDVVQVKLAWMFR"
     gene            complement(1118..2101)
                     /locus_tag="VF516_31425"
     CDS             complement(1118..2101)
                     /locus_tag="VF516_31425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112301.1"
                     /translation="MLAWRGCASRSGTTREDRDEDRDEDRDRDGDGDAELERVREQVR
                     RHRPAPAPGRWLDIVAPAFTIPPNANHAYCFVTHLPNTETLAIRRWRSHMSGGIRDIA
                     VFLGPSATTQTPEVRGAPCDRLVAPVPSEALAFLSHTSTGELELPPNDGAGHPVAQLA
                     EPGALLVVEMQIINPTERELPAQLALHVEAVRRGIVVTPMSSVIAFTRDLRIPAGTAA
                     APGGAMVTGSCAIPASMKLFGVTSYTHHRGAHTFVKDGNAVLLDSTAWDHPDTPAWWT
                     GYSLKNAAFDYSCEYWNPTSQPVTLGGLPDAELCVIVGYVFPAQRPILCPN"
     gene            2372..2494
                     /locus_tag="VF516_31430"
     CDS             2372..2494
                     /locus_tag="VF516_31430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112302.1"
                     /translation="MQQFQILTGAAPGEPSLVGRGPIMAVIGELGAWTGNPRWH"
     gene            2550..3236
                     /locus_tag="VF516_31435"
     CDS             2550..3236
                     /locus_tag="VF516_31435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8112303.1"
                     /translation="MEYVIPAPPIPSLPVEGSHARFPVHRIYCVGRNYADHAREMGGD
                     PDREPPFFFCKPADAIVADGHDFPYPGQTEDVHHEMEFVVALGKGGADIAVDRALDHV
                     YGYAAGLDMTRRDLQAAAKKLGRPWDTGKGFDCAAPCGRIMPASRIGHPDRGAIWLKV
                     NGEVRQRGDLAQLIWKIPEVIAYLSTLFTLAPGDLIYTGTPAGVGPVRRGDALEGGVD
                     GVGTLALQVV"
     gene            3370..>6423
                     /locus_tag="VF516_31440"
     CDS             3370..>6423
                     /locus_tag="VF516_31440"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112304.1"
                     /translation="MRAILPVLLVLAIGGAAAAAPEPAVSPKLFAGLHWRSIGPYRGG
                     RALAVAGVPGDPFTYYFGGVAGGVWKTTNGGLSWTPMFDQQDISSIGAIAVAPSDPNV
                     VYVGSGEACVRGNISYGSGVYRSLDAGRTWTSLGLRDTRHIGRVIVDPKDPNRVFVAA
                     LGHAFGPNPERGVFRTTDGGAHWQKVLFKDADTGAIDVQFDPSNASVLYAALWQVRRL
                     PWNLSSGGPGSGLYKSTDGGTTWKRLSGNGLPAGIYGRIGIAVSPVDPSRVYASVEAA
                     DGGLYRSDDAGGTWTRVNSDERFRQRAWYFSHIFADPRQLDTVYALNTGMFRSVDGGK
                     TFDLLPAPHGDHHGLWIDPTDPRRMINGNDGGATLTVDGGRTWTSQDNQPTAQFYHVI
                     TDDSFPYRVYGTQQDNSSVGIKSYGDGGVIDERDWFEFGGETGWIAPDPRNPEVIFSS
                     NEHTLLRYDHTGNLYQDISVSPLDVSGRGAAELAHRFQWTSPLIAPRNEPGVLYVGGE
                     AVWRSTDDGKTWTAISGDLTRNDKSKQQPSGGPIQLDITSVEYFDTVFVLAASPVARG
                     TLWAGTDDGLVWVTRDTGKAWSKVTPPGMPEWGTVSSIDPSPHAAATAYLTVDRHRLD
                     DLRPYAWKTRDGGATWTSISAGLPAAPVHVVREDPRRPGLLYAGTELGASMSFDDGAH
                     WQPLMAGLPVTPVTDATVHGDDLVVSTNGRGFWILDDIAPLRELAAGAPAIARQPAHL
                     FAPARALRTAYNVFPDKRRPVGDNPPQGAVFDYWLAAEPRGDVTIEIADARGALVRRL
                     SSAAPDRGPDQPPEWVDRVRTPDQLGKQAGMNRFVWDLRWSEPTQIPGAFYQGLPPWG
                     PLALPGSYTVKLTVDGKSQTAPLAIYNDPRSKASDEDLRASFDLQVKTRALIDALHVA
                     VNQIRSTRAQANVLRARLAADPHGKELVASIDGIAKRMDAIEGQLVQVKLASSEGMLR
                     FPAMLNEQLDSFRNTIESDRTPTQPQLQLFAEFAKRLDAQTTAWKA"
BASE COUNT          966 a         2349 c         2250 g          858 t
ORIGIN      
        1 gcctacgtcg gcgcggccga cgccacctgg aagtcgagcg acggcagcgt cgagctcgac
       61 gccatggccg gcggcagcgt gctcgacgcc gcgcgcggca acggcgccac cggctacgcc
      121 cgcgcgaaca agcgctggga cggcggctgg tacgccagcg ccaccaccga ctacgccagc
      181 cgccggttca accccaacga cctcggctac ctcgaggcgt ccgatcacgt cgagctgctg
      241 ggcgaggcag gccgccagtg ggatcgcacg ctcggggtgt tccgcaactg gagcatcgcc
      301 gccaccgcca actacggcca ggaccacgcc ggccgcgcgt tcaacgacaa cgcgggcgtc
      361 cggatcgcga ccggcttcac caacgactgg accgcgtacg ccggagccgc cgacagcttc
      421 ccgcaccacg acgaccgcga gctgcgcgcg ttcctcgatc cggtcaagaa gtacctgcac
      481 cacggccacg agcccaacgc catcgccggg ttcacgagca cgcaccgcag cgcatggtac
      541 gccggcctcg atctgtcggt gacgcactac gccggcgggc cgacctacac cgccaccttc
      601 aaccagacga tccatccgtc gagccggttc gagctcgagc tcgtcaccga tctcgcggtg
      661 gcgcgcggcg agcagcactg gctcgagacc cagggcgaca cgccgatcgt cgggctgcgc
      721 cggctggtcc agctcagcca gatcgtccgg gcgtcgtacg cgttcacccc gaacctcacg
      781 ctgcaggcct acacgcagct cctgctcgcg acctggcact accgcgacct ggcccgctac
      841 gtcgacgaca acacgctcgc cccgggcgcg atggccggga ccacgagctt ctcggccagc
      901 aacttcaacg tcaacggcgt gctgcgctgg gagatccgcc cgggctccac gctgtacgcg
      961 gtgtacaccc acggcgcgtt cgacggcgag gtgttccacc gcgacgcgac gctgagcagc
     1021 cgctcggtct ccgcgctgct gcgcgcgccg tcggacgacg tcgtgcaggt caagctggcc
     1081 tggatgttcc ggtgatgccg acggtcgagt cgtccgctca gttcggacag aggatcggtc
     1141 gctgtgcggg gaacacgtac ccgacgatga cgcacagctc tgcgtccggc aggccgccga
     1201 gcgtcacggg ctgcgaggtc ggattccagt attcacagct gtaatcgaat gccgcgttct
     1261 tcagcgagta tccggtccac catgccgggg tgtcgggatg atcccaggcg gtgctgtcga
     1321 gcaacacggc attgccatcc ttcacgaagg tgtgcgcgcc gcggtgatgg gtatagctcg
     1381 tcaccccgaa cagcttcatg ctcgccggga tcgcacagct cccggtgacc atcgcgcccc
     1441 ccggcgcggc ggcggtcccg gccgggatgc ggaggtcacg ggtgaacgcg atcacggagg
     1501 acatgggggt caccacgatc cctcggcgga ccgcctcgac atgcagcgcg agctgcgccg
     1561 gcagctcgcg ctcggtcgga ttgatgatct gcatctcgac gaccagcagc gcgccgggct
     1621 cggcgagctg ggcgaccggg tgtcccgcgc cgtcgttcgg ggggagctcg agctcgccgg
     1681 tggaggtatg ggacaggaag gcgagcgcct cggacggcac cggcgcgacc agccggtcgc
     1741 acggcgcgcc tcgaacctcg ggcgtctggg tcgtggcgga aggcccgagg aacaccgcga
     1801 tgtcccggat cccgcccgac atgtgggacc gccagcgccg gatcgccagc gtctcggtgt
     1861 tcggcaggtg agtgacgaag cagtaggcat ggttcgcgtt cggcgggatc gtgaacgccg
     1921 gagcgacgat atccagccac cgaccggggg cgggggccgg ccggtgccgc cggacctgct
     1981 cgcggacgcg ctcgagctcg gcgtcgccgt ctccgtcccg gtcccggtct tcgtcgcgat
     2041 cctcatcgcg gtcctcgcgc gtggtgcccg accgcgatgc gcaaccgcgc caggcgagca
     2101 ccccgatggc gaccagggcg agcgcgccgg cgatcagctt gcgggcgagg gtgtcgagga
     2161 tgcggcgagc ctagctgctc gcgggccggc cgcgcaacac cgcccgccac gaggtctgga
     2221 cccatggcct tcgcctccgg tgctacggta cgcgcagcga agcgatcgac gtgctgcagc
     2281 gcgacgacca gctgcacgtg ctggtcgacc tcggccaggt ccggtcaacg cggggcttga
     2341 cccgacgcgg attacgcgga cgatccagac catgcagcag ttccagatcc tgaccggggc
     2401 ggcgcccggc gagccctcgc tggtcggccg cgggccgatc atggcggtga tcggcgagct
     2461 cggcgcctgg accggaaatc cgcggtggca ctgacgcacc cgggcaggat cgcaaccgac
     2521 cgcgtaccgc cgtcatcgaa gaggtctcca tggaatacgt catcccagca ccaccgatcc
     2581 cgagccttcc ggtcgagggc agccacgccc ggttcccggt gcaccgcatc tactgcgtcg
     2641 gccgcaacta cgccgaccac gcgcgcgaga tgggcggcga cccggaccgc gagccgccgt
     2701 tcttcttctg caagccggcg gatgcgatcg tcgccgacgg ccacgacttc ccctacccgg
     2761 gccagaccga ggacgtccac cacgagatgg agttcgtcgt cgcgctcggc aagggcggcg
     2821 cggacatcgc ggtggaccgc gcgctcgacc acgtgtacgg ctacgccgcc gggctcgaca
     2881 tgacgcgccg cgatctgcag gccgccgcga agaagctggg ccggccgtgg gacaccggca
     2941 agggcttcga ttgcgccgcg ccgtgcgggc ggatcatgcc ggcgtcgcgg atcggccacc
     3001 cggaccgcgg ggcgatctgg ctcaaggtga acggcgaggt ccgccagcgc ggcgatctgg
     3061 cgcagctgat ctggaagatc cccgaggtga tcgcgtacct gtcgacgctg ttcacgctgg
     3121 cgccgggcga cctgatctac accggcacgc cggccggcgt cgggccggtg cggcgcgggg
     3181 acgcgctgga gggcggtgtc gacggcgtgg ggaccctggc gctccaggtc gtctagccac
     3241 ctcgccggag gaccgcatgc cgctcagcca tctcgagcgg aacgcgaggt gccgccacgg
     3301 cagcacaggt cccgccacgc atggccggcg cagcgggttt cgcggcgcca gatcggcgat
     3361 agtcgggccg tgcgtgccat cctccctgtc ctccttgtcc tcgcgatcgg cggtgcagcg
     3421 gccgcggcgc cggaacccgc ggtgtcgccc aagctgttcg cgggcctgca ctggcgatcg
     3481 atcgggccgt accgcggcgg ccgggcgctc gcggtcgcgg gcgtccccgg cgatccgttc
     3541 acctattact tcggcggcgt cgctggcggc gtgtggaaga cgaccaacgg cgggctgtcg
     3601 tggacgccga tgttcgacca gcaggacatc tcgtcgatcg gggcgatcgc ggtcgcgccg
     3661 tcggacccca acgtcgtgta cgtcggcagc ggcgaggcgt gcgtccgcgg caacatctcg
     3721 tacggcagcg gcgtgtaccg gtcgctcgac gccggccgga cgtggacctc gctcgggctg
     3781 cgcgacaccc gccacatcgg ccgggtgatc gtcgatccga aggatcccaa ccgggtgttc
     3841 gtcgcggcgc tcggccacgc gttcggcccc aaccccgagc gcggcgtgtt ccgcacgacc
     3901 gacggcggcg cgcactggca gaaggtgctg ttcaaggacg ccgacaccgg cgcgatcgac
     3961 gtgcagttcg atccgtcgaa cgccagcgtg ctgtacgcgg cgctgtggca ggtccggcgg
     4021 ctgccgtgga acctgtcgtc gggcggcccc ggctcggggc tctacaagtc gaccgacggt
     4081 ggcacgacct ggaagcggct gtccggcaac gggctgccgg ccgggatcta cggccggatc
     4141 ggcatcgcgg tgtcgccggt cgacccgtcg cgggtgtacg cgtcggtcga ggcggccgac
     4201 ggcgggctgt accgcagcga cgacgcgggc gggacgtgga cgcgggtcaa ctccgacgag
     4261 cggttccgcc agcgcgcctg gtacttcagc cacatcttcg ccgatcccag acagctcgac
     4321 acggtgtacg cgctcaacac cgggatgttc cgctcggtcg acggcggcaa gacgttcgac
     4381 ctgctgccgg cgccgcacgg cgaccaccac gggctgtgga tcgatccgac cgatccgcgc
     4441 cgcatgatca acggcaacga cggcggcgcg acgctgaccg tcgacggcgg ccggacctgg
     4501 acgagccagg acaaccagcc gaccgcgcag ttctatcacg tgatcaccga cgacagcttc
     4561 ccgtaccggg tgtacggcac gcagcaggac aactcgtcgg tcggcatcaa gtcgtacggc
     4621 gacggcggcg tgatcgacga gcgcgactgg ttcgagttcg gcggcgagac cggctggatc
     4681 gcgcccgatc cccggaaccc cgaggtgatc ttctccagca acgagcacac gctcctgcgc
     4741 tacgaccaca ccggcaacct ctaccaggac atctcggtgt cgccgctcga cgtctcgggc
     4801 cgcggcgccg ccgagctcgc gcatcgcttc cagtggacct cgccgctgat cgcgcccagg
     4861 aacgagcccg gcgtgctcta cgtcggcggc gaggcggtgt ggcgctcgac cgacgatggc
     4921 aagacgtgga ccgcgatctc gggcgacctc acgcgcaacg acaagtccaa gcagcagccg
     4981 tcgggcggcc cgatccagct cgacatcacc agcgtcgagt acttcgacac cgtgttcgtg
     5041 ctcgccgcct cgccggtcgc cagggggacc ctgtgggccg gcaccgacga cggcctggtg
     5101 tgggtcacgc gcgacaccgg caaggcgtgg agcaaggtca cgccgccggg catgccggag
     5161 tggggcacgg tgagctcgat cgacccgtcg ccgcacgccg ccgcgacggc ctacctcacg
     5221 gtcgaccgcc accggctcga cgacctgcgg ccgtacgcgt ggaagacccg cgacggcggc
     5281 gcgacctgga cgtcgatctc ggcgggcctg ccggcggcgc cggtccacgt cgtgcgcgag
     5341 gacccccggc gccccgggct gctctacgcc ggcaccgagc tcggcgcgtc catgtcgttc
     5401 gacgacggcg cgcactggca gccgctcatg gccggcctgc cggtcacgcc ggtgaccgac
     5461 gccaccgtgc acggcgacga cctcgtggtg tcgaccaacg gtcgcgggtt ctggatcctc
     5521 gacgacatcg cgccgctgcg cgagctcgcc gccggcgcgc ccgcgatcgc ccgccagccc
     5581 gcgcacctgt tcgccccggc gcgcgcgctg cgcaccgcgt acaacgtgtt ccccgacaag
     5641 cgccggccgg tcggcgacaa cccgccgcag ggcgcggtgt tcgactactg gctcgcggcc
     5701 gagcccaggg gcgacgtgac gatcgagatc gccgacgcca ggggcgccct tgtgcgccgg
     5761 ctgtcgtcgg ccgcgcccga ccgcgggccg gaccagccgc ccgagtgggt cgaccgcgtg
     5821 cgcacccccg accagctcgg caagcaggcc ggcatgaacc gcttcgtgtg ggatctgcgg
     5881 tggagcgagc cgacccagat ccccggcgcg ttctaccagg gcctgccgcc gtgggggccg
     5941 ctcgcgctgc ccggcagcta cacggtcaag ctcaccgtcg acggcaagtc gcagaccgcg
     6001 ccgctcgcga tctacaacga cccgcgcagc aaggcgtccg acgaggacct gcgcgccagc
     6061 ttcgacctcc aggtcaagac ccgcgcgctg atcgacgcgc tgcacgtcgc ggtcaaccag
     6121 atccgctcga cccgcgccca ggccaacgtg ctgcgcgcgc ggctcgccgc cgatccgcac
     6181 ggcaaggagc tggtcgcgtc gatcgacggc atcgcgaagc ggatggacgc gatcgagggc
     6241 cagctggtgc aggtcaagct cgccagctcc gagggcatgc tgcggttccc ggcgatgctc
     6301 aacgagcagc tcgacagctt ccgcaacacg atcgagtcgg accgcacgcc gacccagccc
     6361 cagctccagc tgttcgccga gttcgcgaag cggctcgatg cgcagaccac cgcgtggaag
     6421 gcg
//
LOCUS       DASVOR010001683         4807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_368010,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001683 DASVOR010000000
VERSION     DASVOR010001683.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368010"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..838
                     /locus_tag="VF516_31445"
     CDS             <1..838
                     /locus_tag="VF516_31445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8112305.1"
                     /translation="APDARNLRALTDDPVVGKASRVDGDERKGVVAFTFDDGPNPETT
                     PAVIDALEKYGIPATFFIVTQRISGKHGEKGRELLQRELADGFLVGSHSVTHVNLKHA
                     AGDQLAREIDASVRTLAAEAHRPIGMFRPPYGSLSAAGRVWLKKLGLTEVLWSVDTLD
                     WKAHDPERLRKKVISMIKHDDGGVVLMHDVKPITAKIIAEVLDDLEAENCARLAAHRE
                     PILPVSLHYFLRDRDGRGAATPRELPAAVQQRTEAYKAALPERCAKRPPAAEPAAQHA
                     TR"
     gene            939..3827
                     /locus_tag="VF516_31450"
     CDS             939..3827
                     /locus_tag="VF516_31450"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8112306.1"
                     /translation="MPLTWDLSGPGLVPGGAREPSSAGSSGHIPISTPEVTDRSQPFE
                     PAEFDAEGVRTIITSPAFELRSRFVGRLPAKAVLQELAARAFERSERGGGGPAGSAGG
                     AGGKAPRGIDRAQSGQPQSGFAVVIGEPGMGKSRILSELAARVRVDHPTTRLFQGVAD
                     ENAHAYGPVARALTARFGIVAGEDPAVSRAKLAAELAEVIDSERLTEVAHLIAHLLRV
                     PYEDSPIVTPSLGAKQREARLFMALKRFLAAESERYPLLVVIENLELCGADTINFLHY
                     LAAGLHDHRVVILGTATGELISRHPAFGEGEVAPVRIEIGPLTPGESEELLRELCKPL
                     DEVPAILTAHVRTMNGSPRAIFELVRLLLETSCIFRDAGAWRINTKTLALTALPRSYD
                     ALVGARLKVMEEIERRVLEMAATVGDTSWFDAILALIRHSQLGSDPDGPTLAQIAGSD
                     DQSRQAVVTAVGRLVEHGWLIEVPQSSIAGERELRFASPNLWSTVYDGMPDATRRGYH
                     AVVARWLELHPEGLSAAAQEEVARHLVLAGAAREAALRYRRAAEAARASYANQHAIQL
                     SDRALDCIGAHDLVTRIQLWRDLASVYELIGDFDAALGAFERMLRLSWVAASKATAAE
                     AFNKMGRVWRRKGDLKLALEYLQRGLELFRAANDTRGAAGSLDDIGRALHMMGRYAEA
                     EAQISEALARHTKDGDQRAIATSLSRLGNVHQDRGHYEAAYECHRDALAIRKATGDRW
                     GQIVAQNNLAALSFELGELSDARAGWLAALPEAEAIGALPLSALILTNLGEVALAENK
                     LEEARRRLENALDIIENIEDRGLESVCCRHLAALEKSQGNAAAARELAERALAVAHQA
                     GLREHEAQAYMTLGDVLSASIHDEEATETGALSPAAIAFGAAVELLANLGNEAALAKA
                     LFVFGRYKAEMGDHADAKDMLRDALMMYSRLGLERRSSEVERLIASMH"
     gene            3989..>4807
                     /locus_tag="VF516_31455"
     CDS             3989..>4807
                     /locus_tag="VF516_31455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC/Alx family metal homeostasis membrane
                     protein"
                     /protein_id="HEX8112307.1"
                     /translation="MIWFGLGFFALVALLLFLDLGVLHRRAAEPSLKSAVGWTLGWIC
                     LGLSFSGIVYLIYEHAWLGARMVAAHGHGHAIGPPSGGGSDAAITYVSGYLLEQALSI
                     DNIFVMSLLFRSFHVPARYQHRVLFWGILGAIGFRISMLGGGAFLAKHFTWVFYLFGA
                     YLGWQGIKLLRGGDDDDGDHDKSLAIRGMRRFVRIVDGDHGGRFTVQIDGRRALTTVA
                     VCLVVIELTDIVFALDSIPAVLAVSQDTFIIITSNIFAIMGLRSLYFVLAGAMAK"
BASE COUNT          705 a         1702 c         1739 g          661 t
ORIGIN      
        1 cgcacccgac gcgcgcaacc tgcgggcgct gaccgacgat ccggtggtcg gcaaggcgag
       61 ccgggtcgac ggcgacgagc gcaagggcgt ggtcgcgttc acgttcgacg acggccccaa
      121 ccccgagacc acgccggcgg tgatcgatgc gctggagaag tacggcatcc cggccacgtt
      181 cttcatcgtc acccagcgga tctccggcaa gcacggcgag aagggccgcg agctgctgca
      241 gcgcgagctc gccgacggct tcctggtcgg cagccactcg gtgacccacg tcaacctgaa
      301 gcacgccgcg ggcgaccagc tcgcgcgcga gatcgacgca tcggtccgga cgctcgcggc
      361 cgaggcccac cgtccgatcg ggatgttccg tccgccgtac ggctcgctca gcgcggccgg
      421 tcgggtctgg ctcaagaagc tcgggctgac cgaggtgctg tggtcggtcg acacgctcga
      481 ctggaaggcg catgaccccg agcggctgcg caagaaggtg atctcgatga tcaagcatga
      541 cgacggcggc gtcgtgctga tgcatgacgt caagccgatc accgccaaga tcatcgccga
      601 ggtgctcgac gacctcgagg ccgagaactg cgcccggctc gccgcccacc gcgagccgat
      661 cctcccggtg tcgctgcact acttcctgcg cgaccgggac ggccgcggtg cggccacgcc
      721 gcgcgagctg ccggcggcgg tccagcagcg gaccgaggcc tacaaggcgg cgctgcccga
      781 gcgctgcgcg aagcggccgc ccgccgcaga gccggccgct cagcacgcca ccaggtagct
      841 cgccgtgagc cgaccgttgt cgcaatcatt cgtcgattga caagccgatc gagaaccact
      901 agcttccgcg cgcctcgaag ggggatgagg aagcgatcat gccgttgacg tgggatctct
      961 cggggccggg gcttgtcccc ggcggcgcgc gcgagccgtc gtccgccggc agcagcggcc
     1021 atatcccgat cagcacgccg gaggtcaccg accgctcgca gccgttcgag cccgccgagt
     1081 tcgacgccga gggcgtgcga acgatcatca cctcgccggc cttcgagctg cggtcgcggt
     1141 tcgtcggccg gctgcccgcg aaggccgtgc tccaggagct ggccgcccgg gccttcgagc
     1201 gaagcgagag ggggggtggg ggccctgccg gctctgccgg cggggcgggg ggcaaagccc
     1261 cccgtgggat cgatcgggcc cagagcgggc agccccagag cggattcgcc gtggtgatcg
     1321 gcgagcccgg catggggaaa agccggatcc tcagcgagct cgcggcgcgg gtccgggtcg
     1381 accacccgac cacccggctg ttccagggcg tggccgatga gaacgcccac gcctacggcc
     1441 cggtcgcgcg cgcgctgacc gcgcggttcg ggatcgtcgc gggcgaggac ccggcggtca
     1501 gccgcgccaa gctcgccgcc gagctcgccg aggtgatcga ctccgagcgg ctgaccgagg
     1561 tcgcgcacct gatcgcgcac ctgttgcggg tgccgtacga ggacagcccg atcgtcacgc
     1621 cgagcctggg cgccaagcag cgcgaggccc ggctgttcat ggcgctcaag cggttcctcg
     1681 ccgccgagag cgagcgctac ccgctgctgg tggtgatcga gaacctcgag ctgtgcggcg
     1741 cggacacgat caacttcctg cactacctcg ccgccggcct gcacgatcac cgcgtggtga
     1801 tcctcggcac cgcgaccggc gagctgatca gccggcaccc cgcgttcggc gagggcgagg
     1861 tcgcgccggt ccggatcgag atcggcccgc tgaccccggg cgagtccgag gagctgctcc
     1921 gcgagctgtg caagccgctc gacgaggtcc cggcgatcct gaccgcgcac gtccgcacga
     1981 tgaacggctc gccgcgcgcg atcttcgagc tggtgcggct gctgctggag accagctgca
     2041 tcttccgcga cgccggcgcg tggcggatca acaccaagac gctcgcgctg accgcgctgc
     2101 cgcgcagcta cgacgcgctg gtcggcgcgc ggctcaaggt gatggaggag atcgagcgcc
     2161 gcgtgctcga gatggcggcc acggtcggcg acacctcgtg gttcgacgcg atcctcgcgc
     2221 tgatccgcca cagccagctc ggcagcgatc ccgatgggcc gaccctggcc cagatcgcgg
     2281 gctcggacga ccagagccgc caggcggtgg tcaccgcggt cggccggctg gtcgagcacg
     2341 gctggctgat cgaggtcccg cagtcgtcga tcgccggcga gcgcgagctg cggttcgcca
     2401 gcccgaacct gtggtcgacc gtctacgacg ggatgcccga cgcgacccgc cgcggctacc
     2461 acgccgtggt cgcgcgctgg ctggagctcc accccgaggg gctgagcgcg gcggcgcagg
     2521 aggaggtcgc ccgccacctc gtgctcgccg gggcggcgcg cgaggccgcg ctgcgctacc
     2581 ggcgggccgc cgaggccgcg cgcgccagct acgccaacca gcacgcgatc cagctgtccg
     2641 accgcgcgct cgactgcatc ggcgcgcacg acctggtgac gcggatccag ctgtggcgcg
     2701 atctggcgtc ggtctacgag ctgatcggtg acttcgacgc cgcgctcggc gcgttcgagc
     2761 gcatgctgcg gctgtcgtgg gtcgccgcga gcaaggcgac cgccgccgag gcgttcaaca
     2821 agatgggccg ggtgtggcgc cgcaagggcg atctcaagct cgcgctggag tacctgcagc
     2881 gcggcctcga gctgttccgc gccgccaacg acacccgcgg ggcggcgggc tcgctcgacg
     2941 acatcggccg cgcgctccac atgatggggc gctacgccga ggccgaggcc cagatcagcg
     3001 aggcgctggc ccgccacacc aaggacggcg accagcgcgc gatcgcgacc tcgctgtcgc
     3061 ggctcggcaa cgtccaccag gaccgcggcc actacgaggc ggcctacgag tgccaccgcg
     3121 acgcgctcgc gatccgcaag gcgaccggcg accgctgggg ccagatcgtg gcgcagaaca
     3181 acctcgccgc gctgtcgttc gagctcggcg agctgtccga cgcccgcgcc ggctggctgg
     3241 ccgcgctgcc cgaggccgag gcgatcgggg cgctgccgct cagcgcgctg atcctgacca
     3301 acctcggcga ggtcgcgctc gccgagaaca agctcgagga ggcgcggcgc cggctcgaga
     3361 acgcgctgga catcatcgag aacatcgagg accgcgggct ggagagcgtg tgctgccgcc
     3421 acctcgcggc gctcgagaag agccagggca acgccgcggc cgcccgcgag ctcgccgagc
     3481 gcgcgctggc ggtggcccac caggccggcc tgcgcgagca cgaggcccag gcctacatga
     3541 cgctcggcga cgtgctgtcg gcgagcatcc acgacgagga ggccaccgag accggcgcgc
     3601 tgtccccggc ggcgatcgcg ttcggcgccg cggtcgagct cctggccaac ctcggcaacg
     3661 aggccgcgct cgccaaggcg ctgttcgtgt tcggccgcta caaggccgag atgggcgacc
     3721 acgccgacgc caaggacatg ctgcgcgacg cgctgatgat gtacagccgg ctcggcctgg
     3781 agcgccggtc gagcgaggtc gagcggctga tcgcctcgat gcactagcag catagagggg
     3841 gcacaggccg atcaggtgca ggtccgcgcg acgcgacgcg acgcggaggg gtgaatggac
     3901 gggctctgcc cggccagagg ggacgggaaa gtcccctatc taacattgcc aagggccggc
     3961 cgacccggca tggtctgggt tcgcggcgat gatctggttt gggcttggtt tcttcgcgct
     4021 ggtcgcgctg ctgctgttcc tcgatctcgg ggtcctgcat cgccgggccg ccgagccatc
     4081 gctcaagagc gccgtgggct ggaccctcgg ctggatctgc ctgggcctgt cgttctccgg
     4141 catcgtctat ctgatctacg aacatgcctg gctcggcgcg cggatggtgg ccgcgcacgg
     4201 ccacggccat gcgatcggcc cgccgtcggg gggcggcagc gacgcggcga tcacctacgt
     4261 ctcgggctac ctgctggagc aagcgctgtc gatcgacaac atcttcgtga tgtcgctgct
     4321 gttccgcagc ttccacgtgc cggccaggta ccagcaccgc gtgctgttct ggggcatcct
     4381 cggcgcgatc gggttccgga tctcgatgct cggcggcggc gcgttcctcg ccaagcactt
     4441 cacctgggtg ttttacctgt tcggagcgta cctcggctgg caggggatca agctgctgcg
     4501 cggcggcgac gacgacgacg gcgaccacga caagagcctg gcgatccgcg ggatgcggcg
     4561 gttcgtgcgc atcgtcgacg gcgaccacgg cgggcggttc acggtccaga tcgacggccg
     4621 gcgcgcgctg accacggtcg cggtgtgcct ggtggtcatc gagctgaccg acatcgtgtt
     4681 cgcgctcgac tcgatcccgg cggtgctcgc ggtcagccag gacacgttca tcatcatcac
     4741 ctcgaacatc ttcgcgatca tggggctgcg ctcgctgtac ttcgtgctcg ccggcgcgat
     4801 ggcgaag
//
LOCUS       DASVOR010001684         4140 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_425722,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001684 DASVOR010000000
VERSION     DASVOR010001684.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4140)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4140)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4140
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425722"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..889)
                     /locus_tag="VF516_31460"
     CDS             complement(<1..889)
                     /locus_tag="VF516_31460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112308.1"
                     /translation="MTGKIMVVDDNAATRRMVRNALIRNGHDVIEAADGQTALELMKT
                     EQPRVVLADLALPDTDGYQLVGELRRLAHGTDVSILAFSGFLSDFDEARISRVGFDDI
                     IAKPISPARLVPLIEAHLPVAAPTSAQFGRGRRLVIADDDPMQLKLATFRLSRLGFDI
                     EAVPDGRSALDAVRRRAPDVVVSDVMMPHVDGFSLAMAVRQEPALSKVPVVLMTSSYV
                     DPADRELARRAGASDLVPRTPELSELIDSLRTTLASNQDAPPLDAGALDELEKEHTRR
                     VFRQLERQVLLNTGLAKRCS"
     gene            complement(886..1272)
                     /locus_tag="VF516_31465"
     CDS             complement(886..1272)
                     /locus_tag="VF516_31465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112309.1"
                     /translation="MAGERILIVDDNATNLKLVAYLMKANGYTVDTALDAESAIEAIR
                     HHRPDVILMDIQLPGIDGLELTRRLKADPATRDIVIVAVTAYAMKGDQAKALAAGCDD
                     YITKPIDTRSLPETIARHLARRLEVS"
     gene            complement(1272..3230)
                     /locus_tag="VF516_31470"
     CDS             complement(1272..3230)
                     /locus_tag="VF516_31470"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112310.1"
                     /translation="MPLQLLVAEDSEDDFEILLRELRKGGYAVSAERVKSTAELDAAL
                     VRPWDLLISDWIMPGFGGLQVLEALTARGLDLACIIVSGTPGEEPAVEALRAGALDFL
                     SKDKPRRIVPAVQRALREAADRRARVRVERELRYSERRFRTGFELAPEPLLSYDLDHR
                     AIVDANAKAAALFRCSRDDLRSQDLAALSPARQPDGRSSVDAAEHYLQRTLAGDSPVF
                     AWTFQAAGEPIPTETRIINLATVGHNLAQINIVDLRERLRAEEIRRRSVDLEIQNRRI
                     QEANRLKSEFLANMSHELRTPLNAIIGFAELLHDGQVDPASPTHKEFLGDILTSGRHL
                     LQLINDVLDLAKVEAGKLEFRPEPVDVPRLVSEVIAILRTTAAHKQIRLDTQVDPSVA
                     ALTVDPARLKQVAYNYLSNALKFTPTGGNVTLRIKPNGPDQFRLEVEDTGIGIAPADL
                     GRLFVEFQQLEAGAAKRHQGTGLGLALTRRLVEAQGGSVGVKSAVGAGSTFHACLPRH
                     AIATPRTATPILFAPNREGARTVLIVEDDARDQLQLVAALESAGYAVELAANGSEAIA
                     RWRSRPYDAATIDLLLPDMSGLELLSALRGEAANRTTPIIVITVVPDARLVAGFTVHD
                     VLHKPLDRERLLASLARAGLPRHQPERS"
     gene            complement(3353..>4140)
                     /locus_tag="VF516_31475"
     CDS             complement(3353..>4140)
                     /locus_tag="VF516_31475"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112311.1"
                     /translation="RSELDAAEARAAKALAETRAAMAEELARANADLALATRELEAFS
                     YSVSHDLRAPLRTISAFTYALIEDFGDAIDDKARDHIRRVLAATARMSDLIDALLELS
                     RISRAPIGRHRVDLSQLAAVVLDELARRDPARAVSLDIAPGLVIDADGRLMRILLDNL
                     LGNAWKFTTRTDPARIAVGRETGAEGETVFFVRDNGAGFDPSQADRLFKPFQRLHHDG
                     EFAGTGIGLATVRRIVERHGGKIWAEGSIGGGAKFSFTIPANR"
BASE COUNT          550 a         1430 c         1540 g          620 t
ORIGIN      
        1 ccgagcaccg cttggccagc ccggtgttga gcaggacctg gcgctcgagc tggcggaaca
       61 cccggcgggt gtgctccttc tccagctcgt cgagcgcgcc ggcgtcgagc ggcggcgcgt
      121 cctggttcga cgccagggtc gtgcgcaggc tgtcgatcag ctcggacagc tcgggggtgc
      181 gcggcaccag gtcgctggcg ccggcgcgcc gggcgagctc gcggtcggcg gggtcgacgt
      241 agctcgaggt catcagcacg accgggacct tgctcagcgc cggctcctgg cgcaccgcca
      301 tcgccaggct gaacccgtcg acgtgcggca tcatgacgtc ggacaccacg acgtcggggg
      361 cgcgccggcg caccgcgtcc agcgcggacc gcccgtcggg caccgcctcg atgtcgaacc
      421 ccagccgcga cagccggaac gtcgccagct tgagctgcat cgggtcgtcg tcggcgatca
      481 ccagccggcg gccgcggccg aactgcgcgc tggtcggcgc cgccaccggc aggtgggcct
      541 cgatcagcgg gaccaggcgc gccggcgaga tcggcttggc gatgatgtcg tcgaacccga
      601 cccgggagat ccgggcctcg tcgaagtcgg acaggaagcc ggagaacgcg aggatcgaga
      661 cgtcggtgcc gtgcgcgagc cggcgcagct cgccgaccag ctggtagccg tcggtgtcgg
      721 gcagcgcgag gtcggccagc acgacgcgcg gctgctcggt cttcatcagc tccagcgcgg
      781 tctggccgtc ggcggcctcg atcacgtcgt ggccgttgcg gatcagcgcg ttgcgcacca
      841 tccggcgggt ggccgcgttg tcatcgacca ccatgatctt gccggtcatg acacctccag
      901 ccgcctggcg aggtgccgcg cgatggtctc gggcagcgag cgggtgtcga tcggcttggt
      961 gatgtagtcg tcgcagccgg ccgccagcgc cttggcctgg tcgcccttca tcgcgtacgc
     1021 cgtcaccgcg acgatcacga tgtcgcgggt cgccggatcg gccttgagcc ggcgggtcag
     1081 ctccagcccg tcgatcccgg gcagctggat gtccatcagg atgacgtcgg ggcggtggtg
     1141 ccggatcgcc tcgatcgccg actcggcgtc gagcgcggtg tcgaccgtgt agccgttggc
     1201 cttcatcagg tacgccacca gcttgaggtt ggtggcgttg tcgtcgacga tcaggatccg
     1261 ttcgccggcc atcacgagcg ctccggctgg tgtctgggca ggcctgcccg ggcgagcgag
     1321 gcgagcaggc gctcgcggtc gagcggcttg tgcagcacgt cgtgcaccgt gaacccggcg
     1381 accagccggg cgtcgggcac cacggtgatc acgatgatcg gcgtcgtgcg gttggcggcc
     1441 tcgccgcgca gcgcggacaa cagctcgagc ccgctcatgt cggggagcag caggtcgatc
     1501 gtcgcggcgt cgtacggccg cgatcgccag cgcgcgatcg cctcgctgcc gttggccgcg
     1561 agctcgaccg cgtagcccgc gctctccagc gccgcgacca gctgcagctg atcgcgcgcg
     1621 tcgtcctcga cgatcagcac ggtgcgcgcg ccctcgcggt tcggcgcgaa caggatcggg
     1681 gtcgcggtcc gcggcgtcgc gatcgcgtgg cgcggcaggc aggcgtggaa cgtgctgccc
     1741 gcgccgaccg cgctcttgac cccgaccgag ccgccctggg cctcgaccag ccggcgggtc
     1801 agcgcgagac cgaggcccgt gccctggtgg cgcttggcgg cgccggcctc gagctgctgg
     1861 aactcgacga acagccggcc gaggtccgcc ggcgcgatgc cgatcccggt gtcctcgacc
     1921 tcgagccgga actggtcggg gccgttcggc ttgatccgca gcgtgacgtt gccgccggtc
     1981 ggcgtgaact tgagcgcgtt ggacaggtag ttgtaggcga cctgcttgag ccgcgccgga
     2041 tcgacggtca gcgccgcgac gctggggtcg acctgggtgt cgagccggat ctgcttgtgc
     2101 gccgcggtgg tccgcaggat cgcgatgacc tcgctgacca gccgcggcac gtcgaccggc
     2161 tcgggccgga actcgagctt gccggcctcg accttggcga ggtcgaggac gtcgttgatc
     2221 agctgcagca ggtggcgccc gctggtcagg atgtcgccga ggaactcctt gtgggtcggc
     2281 gacgccgggt cgacctggcc atcgtggagc agctcggcga acccgatgat cgcgttgagc
     2341 ggcgtgcgca gctcgtgcga catgttggcg aggaactcgc tcttgagccg gttggcctcc
     2401 tggatccgcc ggttctggat ctccagatcg acgctgcgcc ggcggatctc ctcggcgcgc
     2461 agccgctcgc gcaggtcgac gatgttgatc tgcgcgaggt tgtgcccgac ggtggcgagg
     2521 ttgatgatcc gggtctcggt cgggatcggc tcgcccgcgg cctggaacgt ccacgcaaac
     2581 accgggctgt cgccggccag cgtgcgctgc aggtaatgct cggccgcgtc gaccgagctc
     2641 cgcccgtcgg gctggcgcgc cgggctgagc gcggcgaggt cctgcgagcg caggtcgtcg
     2701 cgcgagcacc ggaacagcgc cgcggccttg gcgttggcgt cgacgatcgc ccggtggtcg
     2761 aggtcgtagg acagcagtgg ctcgggcgcg agctcgaacc cggtgcggaa ccggcgctcg
     2821 ctgtaccgca gctcgcgctc gacccggacc cgggcgcggc gatcggcggc ctcgcgcagc
     2881 gcgcgctgga ccgcggggac gatccggcgc ggcttgtcct tggacaggaa gtcgagcgcg
     2941 ccggcgcgca gcgcctcgac cgcgggctcc tcgccgggcg tgcccgagac gatgatgcac
     3001 gcgaggtcga gcccgcgcgc ggtgagcgcc tccagcacct ggagcccgcc gaacccgggc
     3061 atgatccagt cggagatcaa caggtcccac gggcggacca gcgccgcgtc gagctcggcg
     3121 gtcgacttga cgcgctccgc ggagaccgcg tatccgccct tgcgcagctc gcgcaacaga
     3181 atctcgaagt cgtcctccga gtcctcggcg acgagcagct gcagtggcac gttggcggac
     3241 gttgctctct gcaccaaacg accagacctg aaacaaccgc taggtagaaa acgataggcc
     3301 tgttgtactt gccgaggcaa gcccctcacg gacggttcac gacgtaccgc ggttaacgat
     3361 tcgccgggat ggtgaacgag aacttcgcac cgcccccgat cgatccttcc gcccagattt
     3421 ttccaccgtg gcgctcgacg atgcgccgca ccgtcgcgag gccgatgccg gtgccggcga
     3481 actcgccgtc gtggtgcagg cgctggaacg gcttgaacag ccggtcggcc tgcgaggggt
     3541 cgaacccggc gccgttgtcg cggacgaaga acacggtctc gccctccgcg ccggtctcgc
     3601 ggccgaccgc gatccgcgcg ggatcggtgc gggtcgtgaa cttccacgca ttgccgagca
     3661 ggttgtcgag caggatccgc atcagccggc cgtcggcgtc gatcaccagg ccgggcgcga
     3721 tgtcgaggct gaccgcgcgc gccggatctc gccgcgccag ctcgtcgagc acgaccgccg
     3781 cgagctggct gaggtcgacc cggtggcggc cgatcggcgc ccggctgatc cgcgacagct
     3841 cgagcagcgc gtcgatcagg tcggacatcc gcgcggtggc ggccagcacg cggcggatgt
     3901 ggtcgcgcgc cttgtcgtcg atcgcgtcgc cgaagtcctc gatcagggcg taggtgaacg
     3961 cgctgatcgt gcgcagcggg gcgcgcaggt cgtgcgacac cgagtagctg aacgcctcga
     4021 gctcgcgggt ggccagcgcc aggtcggcgt tggcgcgggc gagctcctcg gccatcgcgg
     4081 cgcgggtctc ggcgagcgcc ttggcggccc gggcctcggc ggcgtcgagc tcgctgcgca
//
LOCUS       DASVOR010001685         2120 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_251415,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001685 DASVOR010000000
VERSION     DASVOR010001685.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251415"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..1199)
                     /locus_tag="VF516_31480"
     CDS             complement(90..1199)
                     /locus_tag="VF516_31480"
                     /inference="COORDINATES: protein motif:HMM:NF021759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2786 domain-containing protein"
                     /protein_id="HEX8112312.1"
                     /translation="MCDAESTSAAALSAALEAALLRELRARYDWDNRTRFGGRLVPPV
                     LVLSDATSHLGRWHSATRTLELSRALVLHRPWLEVASVLEHEMAHQFVDEVLRVRGET
                     AHGETFRRVCAERGIDHRAAGAPTAASAVEGAAEGGDTDRVLDRIRKLLALAGSPNQH
                     EAEIAMRKAHELMLRHNIEVANTRAERSYEVRHLGDPGRRGTRVESEIAALLSELFFV
                     KVIRVPVYLPRTGKSGLVYEIAGTHANVEMASHVWAFLLATAERLWAENRHDTRVRSG
                     RDRLVYQSGVIGGFREKLLAERTGLKQTGLVWVGDRDLDRFYRARHPNMTTRRHHVRI
                     NSAHAAGREAGRTVVLHKPVERGGSAGSGPRLLRG"
     gene            1459..>2120
                     /locus_tag="VF516_31485"
     CDS             1459..>2120
                     /locus_tag="VF516_31485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112313.1"
                     /translation="MAVGLAGLTGCATAGGLDEPQDADPEASAIAPKQSCAKVRCGSP
                     DAPNVLFPGNPACNGGGCERGLAGDDLYIPPRNGAPWQDTYGLGTRMPDTLSGYSSGR
                     IALLRRLALIGDGAHAVLLDPSYPDGLRDFTGRGPQRGEDIVKAWLVADPARTFLLVY
                     STRSIGWSEYAALQTSEVAARVKVCAVDAPHLQVPAVPHVHDALVDPDGWDNGRCRWG
                     MAT"
BASE COUNT          261 a          725 c          842 g          292 t
ORIGIN      
        1 ggcggatcca gctcgcactg ccgaacggtc tcccggggcg gtaggtcgtc gatccacgcg
       61 gtcagccgcg cagcggcgcg ggcccctggt cagccgcgca gcaggcgcgg cccggatccc
      121 gcggagccac cgcgctcgac cggcttgtgc aggaccacgg tgcgaccggc ctcgcggccg
      181 gcggcgtggg cgctgttgat ccggacgtgg tggcggcgcg tggtcatgtt ggggtggcgc
      241 gcgcggtaga accggtcgag atcgcgatcg ccgacccaga ccaggccggt ctgcttgagc
      301 ccggtgcgct cggcgagcag cttttcacgg aacccgccga tcacgccgga ctggtagacc
      361 agccgatcgc ggccgctgcg gacccgggtg tcgtggcggt tctcggccca cagccgctcg
      421 gcggtggcga gcaggaacgc ccagacgtgg ctcgccatct cgacgttggc gtgggtgccg
      481 gcgatctcgt agaccaggcc cgacttgccg gtgcgcggca ggtagaccgg gacgcggatc
      541 accttgacga agaacagctc ggacaagagc gccgcgatct cggactcgac gcgcgtgccg
      601 cgccggcccg gatcgccgag gtggcggacc tcgtagctgc gctcggcgcg cgtgttggcg
      661 acctcgatgt tgtggcgcag catgagctcg tgggccttgc gcatcgcgat ctcggcctcg
      721 tgctggttcg ggctgccggc gagcgcgagc agcttgcgga tccggtcgag cacgcgatcg
      781 gtgtcgccgc cctcggcggc gccttctacg gcggatgctg cggtcggggc gccggcggcg
      841 cgatggtcga tgccgcgctc ggcgcacacc cggcggaacg tctcgccgtg cgcggtctcg
      901 ccgcggaccc ggagcacctc gtcgacgaac tggtgggcca tctcgtgctc cagcacgctg
      961 gcgacctcga gccacggccg gtgcaggacc agcgcgcgcg acagctccag cgtccgggtc
     1021 gcgctgtgcc agcgaccgag gtggctcgtg gcatcggaca gcacgaggac gggcgggacg
     1081 aggcggcctc cgaaccgcgt gcggttgtcc cagtcgtagc gcgcgcgcag ctcgcggagc
     1141 agcgcggctt ccagcgcggc ggacagggcc gcggcggagg tggattcggc atcgcacatc
     1201 gccctcggat agcacggggc tctgtcaccg gccgtgacgg cggcgctcgc ctcgtgggtc
     1261 gcctcgtggg tcgtcgtcgg cagggtgcga tggcgctcgg gtcgcgcgac ctggcatgtg
     1321 gagttgccgg cagcggcggc ggccggacgc gccgctgcga tcctgggcgg atagggcgcc
     1381 gccggccgga gacgccgcgg catcgcgcgt gcactgcggg gcggtatgcg acgtggccgt
     1441 gtgcgatgga aggtgtgtgt ggcggtgggg cttgcggggc tgaccggctg cgcgacggca
     1501 ggtgggctcg acgagccgca ggatgccgat cccgaggcca gcgcgatcgc gccgaagcag
     1561 agctgtgcga aggtccggtg cggcagcccg gacgcgccga acgtgctgtt cccgggcaac
     1621 ccggcgtgca atggcggcgg ctgcgagcgc ggtctcgccg gcgatgacct gtacatcccg
     1681 ccgcgcaacg gcgcgccgtg gcaggacacc tacgggctcg ggacgcggat gcccgatacg
     1741 ctgtcgggct acagctcggg gcggatcgcg ctgctgcgcc ggctcgcgct gatcggcgac
     1801 ggcgcgcacg cggtgctgct cgacccgtcg taccccgacg ggctgcgcga cttcaccggc
     1861 cgcgggcccc agcgcggcga ggacatcgtg aaggcgtggc tggtcgccga cccggcgcgg
     1921 acgttcctgc tcgtctacag cacgcgcagc atcggctggt ccgagtacgc cgcgctgcag
     1981 accagcgagg tcgcggcgcg ggtcaaggtc tgcgcggtcg acgcgccgca cctgcaggtg
     2041 ccggcggtgc cgcacgtgca cgacgcgctg gtcgatcccg atggctggga caacggccgc
     2101 tgccgctggg gcatggcgac
//
LOCUS       DASVOR010001686         2191 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_368023,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001686 DASVOR010000000
VERSION     DASVOR010001686.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368023"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            735..2036
                     /locus_tag="VF516_31490"
     CDS             735..2036
                     /locus_tag="VF516_31490"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEX8112314.1"
                     /translation="MAAAASGDSIAVLEGDYADNVAVSKSITLLGGFAGTFATRDPAG
                     HVTTIRPTSAGPMNAPIIGLSSGSLVLDGLSLVEGQNNDHGGGVRVDGGTARIQNCVI
                     ARNNAFFTGGGIYIHGGASVTLIGTRIEDNTVGGQDNCAGAGITVESSTVTLRDSTIQ
                     RNRILQNMGSGGGMVLVDSVATLTNNQIQNNHAADQGQVGSGGGIATFRSQVTINGGT
                     ISHNDVGNTTGTGAGLDLIGGSVRLDGVTITSNATGALAGGGAGAIHAAGTKLILTSS
                     VIASNIGNAGAIVADPQGSTAIVNCTIADNTARGILASSTLTLVNSVLLRNGVGLELS
                     QSPQTIIQQNAFFGNTTNAVGTTLPASNLTVDPRIDGTLHLMADSPLIDTGMAGPIST
                     PDTTAPPVDPPSKDIDGDPRVVMGKTSLLPDIGADEYRPMR"
BASE COUNT          376 a          737 c          747 g          331 t
ORIGIN      
        1 gccgtcagcg cgccgacgat caatgcaggc agcatgacca aagcgtagca cgttgcgtca
       61 ccggggctgc gtacgcagtg cagacagcac ctactccccc atcgagcgcg ctctcgtcga
      121 ctcctcgaag atgcagcatg ggctccaaac tggcgtcgac gggggctgcc gcgctctgcc
      181 gcgcacacct gtcctcgggc tgtctgtgac gcgaggccgc gtgatatttt cgtgcgatgc
      241 caagtacgag gaatccttgg atcaaccccg cggatcgccc cggccggcga gcgcacgcct
      301 cgggcgcgtt gctcctcgcg atcagtgcgg tcaccggttg cggcttccca cgcccacccg
      361 acgtccccgg agatgcttcg acgcagttcg ccgtcgcctc gaccgatccg gcggcacatg
      421 cagcgagcgt cgatcctcgg accttgatcc gcgtgacgtt ctcgagcagc ctcggcggca
      481 ccgtgccgca gctcgcggtc gcgaccgcgg atggggcggt tgcgggcaac gtgtccgtcg
      541 acggcgcggt cgtcacgttc caccccgccg cgccgctcgg aaacgggacg ccggtcgcgg
      601 taacggtggt gtcggcgtcc gatgccgcgg gtgacgcgct cgcggcgccg gtgcagttcg
      661 acttcacgac caagaccacc ttgtgtgtca agcctggcgg cggcgacggt tgctacgcgc
      721 ggccgtccga tgccgtggcg gccgccgcca gcggtgactc gatcgccgtg ctggagggcg
      781 actacgcgga caatgtcgcg gtcagcaagt cgatcacgct gctcggtggg ttcgccggca
      841 cgttcgcgac gcgcgatccg gcaggccacg tcacgaccat ccggccaacg tccgcgggtc
      901 cgatgaacgc gccgatcatc ggcctgtcca gcgggtctct cgtgctcgac gggctgtccc
      961 tggtggaagg ccagaacaac gaccacggtg gcggagtacg cgtcgacggt ggaaccgcga
     1021 ggatccagaa ttgtgtgatc gcgagaaaca acgcgttctt cacgggcggt gggatctaca
     1081 ttcacggtgg ggccagcgtc accctgatcg gaacccggat cgaggacaac acagtgggcg
     1141 gccaggataa ctgcgccggt gcgggaatca ctgtcgagtc atcgacggtc acgctgcgcg
     1201 acagcacgat tcagcggaat cgtatcttgc agaacatggg gagcggcggc ggcatggtgc
     1261 tggtcgactc cgtcgccacc ctcacgaaca atcaaatcca aaacaaccat gccgcggatc
     1321 aagggcaggt cggaagcggc ggagggatcg cgaccttccg gtcgcaggtc accatcaatg
     1381 gcgggacgat ctcgcacaac gacgttggca acacgaccgg cactggtgcc ggactcgacc
     1441 tgatcggcgg ctcggtgcgg ctggacggcg tgacgatcac cagcaacgcg accggggcgc
     1501 tggctggagg tggcgcaggc gcgatccacg cagctggcac caagctgatc ctcaccagca
     1561 gcgtcatcgc ctcgaacatc ggcaacgcag gagcgatcgt cgcggatccg cagggctcga
     1621 cggcgatcgt gaactgcacg atcgccgaca acacggctcg ggggatcctg gcgagtagca
     1681 ccctgaccct ggtgaacagc gtcctgctgc gcaacggtgt cgggctcgag ctgtcccagt
     1741 cgccacagac gatcatccag cagaatgcgt tcttcggtaa caccacgaac gccgtgggga
     1801 cgacgctccc cgcctcgaac ctcaccgtcg acccgcgcat cgacggcacg cttcatctga
     1861 tggcggactc gcccctaatc gataccggga tggccggccc gatctcgacc cccgacacca
     1921 cggcccctcc ggtggatcca ccgagcaagg acatcgacgg ggatccgcgc gtggtcatgg
     1981 gcaagacctc gctgctgccc gacatcggcg ccgacgaata tcggcccatg cggtgacggc
     2041 acccggcgct attccagccg ctcgcggcag gggtagtcgc aggtcttgcg gtggctgcag
     2101 tcgtaggcgc agccgcaggc cagggcgaag gtggcgagga tctcgtcggc ggggccgtcg
     2161 gggacggcgc ggcgggcggc gcgggtggcg g
//
LOCUS       DASVOR010001687         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_18424,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001687 DASVOR010000000
VERSION     DASVOR010001687.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18424"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1903
                     /locus_tag="VF516_31495"
     CDS             <1..1903
                     /locus_tag="VF516_31495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HEX8112315.1"
                     /translation="RFVTGAMSFGSISAEAHETLAIAMNRLGARSNSGEGGEEPHRFE
                     PDEHGDLRRSAIKQVASGRFGVTTHYLVHAEDLQIKMAQGAKPGEGGQLPGNKIDERI
                     ARVRCSTPGVTLISPPPHHDIYSIEDLAQLIYDLSCVNPTARVSVKLVSEYGVGTIAA
                     GVAKARAGCVVIAGYEGGTGAAPLSSLAHAGLPWELGLAETQQVLVANRLRDRIRVQV
                     DGGLRTSRDVVIAALLGAEEFGMATASLVATGCVMLRKCHLNTCSVGIATQDPALRAR
                     YAGKPEDVIAYFTFVAERVRERLAELGARSLDELIGRVELLRPRPVDHPKARRLDLSA
                     ILAAPAAGPRRFARPVPWDLSNHVDHDLIRRAESAIAGGRPVELAMPIDNARRAVGTL
                     LSGEIARRHGGRGLPDGAIKVRFSGSAGQSFGAFLAPGVTLELCGDANDYIAKGMSGG
                     RIVVYPPASARFAPEHNVIVGNVALYGATAGDLFACGLAGERFAVRNSGARAVVEGVG
                     DHGCEYMTGGVVVVLGAVGRNFAAGMSGGTAYVFDQAQTLRGRCNLEMVELESVVEES
                     DLWLLNSLIEDHVRFTSSPLGRRVLDNWQHLVSRFVKVMPTDYKRVLQARRAASRPRP
                     GQLVVVGGR"
     gene            1903..>2366
                     /gene="gltD"
                     /locus_tag="VF516_31500"
     CDS             1903..>2366
                     /gene="gltD"
                     /locus_tag="VF516_31500"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831769.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEX8112316.1"
                     /translation="MGKPTGFIEWSRLVPKKRAVAERLRDWREIEGSAGAHESDALRH
                     QAGRCMDCGVPFCHQGCPLGNLVPEFADLVWKQRWRDAHLRLASTNNFPEFTGRLCPA
                     PCEAACVLAIDGQPVTIEAIEKTIIERAFAEGWVAPQPPRVRTGQRVAVVGSG"
BASE COUNT          309 a          855 c          886 g          316 t
ORIGIN      
        1 gcggttcgtg accggcgcga tgtcgttcgg ctcgatcagc gccgaggccc acgagaccct
       61 ggcgatcgcg atgaaccggc tcggcgcgcg gtcgaactcc ggcgagggcg gcgaggagcc
      121 gcaccggttc gagcccgacg aacatggcga cctccggcgc agcgcgatca agcaggtcgc
      181 gtcgggccgg ttcggcgtga ccacccacta cctggttcac gccgaggacc tccagatcaa
      241 gatggcgcag ggcgccaagc ccggcgaggg cggccagctg cccggcaaca agatcgacga
      301 gcggatcgcg cgcgtgcggt gctcgacgcc cggcgtcacg ctgatctcgc cgccgccgca
      361 ccacgacatc tactcgatcg aggacctcgc ccagctgatc tacgacctgt cgtgcgtcaa
      421 cccgaccgcg cgggtcagcg tcaagctggt cagcgagtac ggggtcggca cgatcgcggc
      481 cggcgtcgcc aaggcgcggg ccggctgcgt cgtgatcgcc ggctacgagg gcggcaccgg
      541 cgccgcgccg ctgtcgagcc ttgcgcacgc cgggctgccg tgggagctcg ggctggccga
      601 gacccagcag gtgctggtcg ccaaccggct gcgcgaccgg atccgggtcc aggtcgacgg
      661 cgggctgcgc acgtcgcgcg acgtggtcat cgcggcgctg ctcggcgccg aggagttcgg
      721 catggcgacc gcgtcgctgg tcgcgaccgg ctgcgtgatg ctgcgcaagt gccacctcaa
      781 cacctgctcg gtcgggatcg cgacccagga tccggcgctg cgcgcgcgtt acgccggcaa
      841 gcccgaggac gtgatcgcgt acttcacgtt cgtcgccgag cgcgtgcgcg agcggctcgc
      901 cgagctcggc gcgcgctcgc tcgacgagct gatcggccgg gtcgagctgc tccggccgcg
      961 cccggtggac caccccaagg cccggcggct cgacctgtcg gcgatcctcg cagcgcccgc
     1021 ggccgggccg cgccggttcg cgcgcccggt gccgtgggat ctgtccaacc acgtcgacca
     1081 cgacctgatc cggcgcgccg agtcggcgat cgccggcggc cggccggtcg agctcgcgat
     1141 gccgatcgac aacgcgcggc gcgcggtcgg cacgctgctg tccggcgaga tcgcgcggcg
     1201 ccacggcggg cgcggcctgc ccgacggtgc gatcaaggtc cggttcagcg gctcggcggg
     1261 ccagagcttc ggcgcgttcc tcgcccccgg cgtgacgctg gagctgtgcg gcgacgccaa
     1321 cgactacatc gccaagggca tgtcgggcgg acggatcgtg gtctacccgc cggccagcgc
     1381 gcggttcgcc cccgagcaca acgtgatcgt cggcaacgtc gcgctgtacg gcgccaccgc
     1441 gggcgacctg ttcgcgtgcg ggctcgccgg cgagcggttc gcggtgcgca actccggcgc
     1501 gcgcgcggtg gtcgagggcg tcggcgacca cggctgcgag tacatgaccg gcggcgtggt
     1561 cgtcgtgctc ggcgcggtcg gccgcaactt cgcggccggg atgagcggcg gcaccgccta
     1621 cgtgttcgat caggcgcaga cgctgcgcgg ccggtgcaac ctcgagatgg tcgagctcga
     1681 gtcggtcgtc gaggagagcg acctgtggct gctcaacagc ctgatcgagg accacgtgcg
     1741 gttcacgtcg agcccgctcg gccgccgcgt gctcgacaac tggcagcacc tggtgtcgcg
     1801 gttcgtcaag gtcatgccga ccgactacaa gcgggtgctg caggcgcgcc gcgccgcctc
     1861 ccgtccgcgc cccggacagc tcgtcgtcgt gggaggtcgg tgatgggcaa gcccaccggg
     1921 ttcatcgagt ggtcgcggct cgtgcccaag aagcgcgcgg tcgccgagcg gctgcgcgac
     1981 tggcgcgaga tcgaaggctc cgccggcgcg cacgagagcg acgcgctccg ccaccaggcc
     2041 ggccgctgca tggactgcgg cgtgccgttc tgccaccagg gctgcccgct gggcaacctg
     2101 gtccccgagt tcgccgacct ggtgtggaag cagcgctggc gcgacgccca cctgcgcctg
     2161 gcgtcgacca acaacttccc cgagttcacc ggccggctgt gcccggcgcc gtgcgaggcg
     2221 gcctgcgtcc tggcgatcga cggccagccg gtcacgatcg aggcgatcga gaagacgatc
     2281 atcgagcgcg cgttcgccga gggctgggtc gcgccgcagc cgccccgcgt gcggaccggc
     2341 cagcgcgtcg cggtggtcgg ctcggg
//
LOCUS       DASVOR010001688         4704 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_221967,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001688 DASVOR010000000
VERSION     DASVOR010001688.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4704
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221967"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..382
                     /locus_tag="VF516_31505"
                     /pseudo
     CDS             125..382
                     /locus_tag="VF516_31505"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
     gene            510..1580
                     /locus_tag="VF516_31510"
     CDS             510..1580
                     /locus_tag="VF516_31510"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8112317.1"
                     /translation="MTIATKTDLGADAWAIDDEVVRLREWGTDLSYPLPADAEVTIGS
                     SETCTLPLTDPTGCLSRQHARLAREGTRWIAHDLDSKNGVRLDGVRRPKAVLEPGSEL
                     GVGGITLIAESPRLIALRGYLARILGWSSDRSEAVDLAVRSVRLAATRHAALALCGDG
                     DLVPIARGLHRFSLGDDRPFVLCDPRRRATEQAGSLENYPTGMRAFHAAAGGSMCMWI
                     KRLPRDFAEVTDALRDPGTRVHLILCGQRPPDRKELVSAPIEVPPLSSRPDEIERVID
                     EYASDAAVMLRASASFTKVDRDWVRLHSATSLPEIEKGTLRIVALREAGNIARAAQLI
                     GMAHASLGEWIGRRRLPMGNFK"
     gene            2022..4424
                     /locus_tag="VF516_31515"
     CDS             2022..4424
                     /locus_tag="VF516_31515"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8112318.1"
                     /translation="MKILHLSDLHVTHDGRELNQLWGRARPAVAGLRFDFVVISGDLT
                     QRAAATEYAKLKRFLEAEIEPLVVGDRATVKTRIVIVPGNHDVDWKAEVFETLALANA
                     QSEIAEQWFADGLWRPETQPFRIKTGPYGHTSAFQIRADHYQERFAAVQTFLTDYYAG
                     QLVPPHRPFALVDPRGGTGDWQAHVFPEIHVALLGFNSCYRNDRYWHGAQIHEDAITE
                     ARDHVDRHDHARNFLRIGVWHHGMESHRSRPDRLTFENLTALVTSGIKVGFHGHVHKS
                     HTQLHRFITDDFALVSTGTLGAAADDRPDAIANQFSIVDLHRNRLRVDVYESEGLGAY
                     SAREDRRRFMYFDLDVERPHDISKPLRSWAAHVTRRVTLDPDNGTAKVDIEIDDLDLS
                     EQIVLARVASKFCTAPDAFAMVDGQRLPVTRRDDSDWFEYRSNTWTAKHYRRLTWSYR
                     IANAFALTRAEPSLITKRDEFPHLLDGWELWSHRVQFDYDKLTLEFVFDAPDGDYFAG
                     PSGPLIVPIVERRANGGPLKWERLGSEESRATRNFASARRASMSWPSPMANARYGMTF
                     PLANAGDPLPRKFAVATTKLVQLCRSTRNPGNGLRNALSTYLEASLESALELSREQEA
                     FGEHAVAVGNLWTDAEGLLRPCFGFFPPDAWVTQFEAGRGIAGHAFRFARPAAWHRSI
                     TGEFDVIRVPTMTGTRDYEWILCLPILTSPDGTAIGVFGFAGTRDHNTETTNQLAAFA
                     QQIAQRDPAVDTDTFDRFWYVLNASFWYGLADLAGAARLDYGVIDMAKECSTAFLATD
                     PE"
BASE COUNT          684 a         1753 c         1567 g          700 t
ORIGIN      
        1 gtccacgccg cgtgccccgc ggcggtccgc gcgctggcgt gggcgctgcc gttcgtgcgc
       61 gacgcgcggg tctcgccgac cttccaggta ggcgttcgcc ggccgcggcg gccaccgctg
      121 gcgagtcgcc gcgtgcttcg gcctgatcca cggctttggc ttcgccaccg cgctccacca
      181 gctcgccctg ccgacccgcg agctggtccc cgcgctgctc ggctacaacc tcggcgtcga
      241 gctcggccag gtcgcgatcg tgctggccgc cgcgccgctg atcctcaccc tgcaccgcca
      301 ccgccggctc cgcccgatcc tccgcggctt cgccgcgctg atctgcgcgg ccggcatcca
      361 ctggttcttc cagcggctgt aaccggctgc gccggccccg ccccgggtgc gcccggtgtc
      421 aaaaacttga gcccgcctgc ccgcgcgccg caatccgtga cggccgcgcc cgggatgccg
      481 cggttttgat accctatccg caaccacgga tgaccatcgc gaccaagacg gacctggggg
      541 ccgacgcgtg ggcgatcgat gacgaggtcg tccggctgcg cgagtggggc accgacctca
      601 gctacccgtt gcccgccgac gccgaggtca cgatcggctc gtcggagacc tgcacgctgc
      661 cgctgaccga tccgaccggc tgcctgtcgc gccagcacgc ccggctggcc cgcgagggca
      721 cgcgctggat cgcccacgac ctcgacagca agaacggcgt gcggctcgac ggcgtccgcc
      781 ggcccaaggc ggtgctcgag cccggctccg agctcggggt cggcggcatc acgctgatcg
      841 ccgagagccc gcggctgatc gcgctgcgcg gctacctcgc ccgcatcctc ggctggtcga
      901 gcgatcgcag cgaggcggtc gacctcgcgg tgcgctcggt gcggctcgcc gcgacccggc
      961 acgccgcgct cgcgctgtgc ggcgacggcg acctcgtgcc gatcgcgcgc gggctgcacc
     1021 ggttctcgct gggcgatgat cggccgttcg tgctgtgcga cccgcggcgg cgcgccaccg
     1081 agcaggccgg gtcgctggag aactacccga ccgggatgcg cgcgttccac gccgcggccg
     1141 gcggctcgat gtgcatgtgg atcaagcggc tgccgcgcga cttcgccgag gtcaccgacg
     1201 cgctccgcga ccccggcacc cgcgtgcatc tgatcctgtg cggccagcgc ccgccggatc
     1261 gcaaggagct ggtctcggcg ccgatcgagg tcccgccgct gtcgagccgg cccgacgaga
     1321 tcgagcgcgt gatcgacgag tatgcgtccg acgccgcggt gatgctgcgc gcctcggcca
     1381 gcttcaccaa ggtcgaccgc gactgggtgc ggctgcactc ggcgacctcg ctgcccgaga
     1441 tcgagaaggg cacgctccgg atcgtcgcgc tgcgcgaggc cgggaatatc gcgcgcgccg
     1501 cgcagttgat cggtatggcg cacgcatcgc tcggcgagtg gatcggccgc cgccggctgc
     1561 cgatgggaaa cttcaagtag cggcgggtgt cttgccagag ggcgctacgg aaatcacgtg
     1621 cgagatcttc ccactcgctg gcccgttcga atttgcatcg accgcccggt cgggccataa
     1681 ctctagaaaa attcttgctt gtcttgcatg ctgcggtgcg ccgtcgctcg ctccgaagtc
     1741 tttcctagcg gaggcccctc tatcgcaaac tcgccccaga acttgttgtc cgattgttgt
     1801 ccgttcgctc accagaactc ttgcccatgg cgggtcgtag gagcaaactt accttgtgag
     1861 cgtaacgaag aagccccagg actcggtggc cctgtaccag catcgggcga ttcgcgtcga
     1921 tcacgccagc gtccggaagt tgcaggaagt gatcgcgacc gtggaggctc tcaagcagca
     1981 gcgcccgccg gaatccgacg actgacgtcg gtccacgtcg tgtgaaaatc cttcatcttt
     2041 ctgacctgca cgtcacgcac gacggcaggg agctgaacca gctatggggt cgggcgcgcc
     2101 ctgccgtggc ggggctacgg tttgattttg tcgtgatctc cggggacctg acccagcggg
     2161 cggccgcgac ggagtacgcc aagctcaagc gcttcctcga ggccgagatc gagccgctcg
     2221 tcgtcggcga tcgcgcgacg gtcaagaccc gcatcgtgat cgtgccgggc aaccacgacg
     2281 tcgactggaa ggccgaagtg ttcgagaccc tcgcgctcgc caacgcgcag tccgagatcg
     2341 ccgagcagtg gttcgccgac ggcctgtggc ggcccgagac ccagccgttc cggatcaaga
     2401 ccgggccgta cggccacacc agtgcgttcc agatccgggc cgaccactac caggagcggt
     2461 tcgccgcggt ccagacgttc ctgaccgact actacgccgg ccagctggtc ccgcctcacc
     2521 gtccgttcgc gctggtcgac ccgcgcggcg gcaccggcga ctggcaggcc cacgtgttcc
     2581 ccgagatcca cgtcgcgctg ctcgggttca acagctgcta ccgcaacgac cgctactggc
     2641 acggcgcgca gatccacgag gacgcgatca ccgaggcgcg cgatcacgtc gaccgccacg
     2701 accacgcccg caacttcctg cggatcggcg tgtggcacca cggcatggag agccaccgca
     2761 gccggcccga ccggctgacc ttcgagaacc tgaccgcgct ggtcacctcg gggatcaagg
     2821 tcgggttcca cggccacgtc cacaagtcgc acacccagct ccaccgcttc atcaccgacg
     2881 acttcgcgct ggtgtcgacc ggcacgctcg gcgccgccgc cgacgaccgc cccgacgcga
     2941 tcgccaacca gttctcgatc gtcgatctcc accgcaaccg gctgcgggtc gacgtctacg
     3001 agagcgaggg cctgggcgcg tactcggcgc gcgaggaccg ccggcggttc atgtacttcg
     3061 acctcgacgt cgagcggccg cacgacatct ccaagccgct gcggtcgtgg gccgcccacg
     3121 tcacccggcg ggtcacgctc gaccccgaca acggcaccgc caaggtcgac atcgagatcg
     3181 acgacctcga cctcagcgag cagatcgtgc tggcgcgggt cgcgtccaag ttctgcaccg
     3241 cgcccgatgc cttcgcgatg gtcgacggcc agcgcctgcc ggtgacccgg cgcgacgaca
     3301 gcgactggtt cgagtaccgc tccaacacgt ggaccgccaa gcactaccgc cggctgacct
     3361 ggagctaccg gatcgccaac gcgttcgcgc tgacccgcgc cgagccgtcg ctgatcacca
     3421 agcgcgacga gttcccccac ctgctcgacg gctgggagct gtggtcgcac cgcgtccagt
     3481 tcgactacga caagctgacc ctggagttcg tgttcgacgc gcccgacggc gactacttcg
     3541 ccggcccgag cggcccgctg atcgtgccga tcgtcgagcg ccgcgccaac ggcggcccgc
     3601 tcaagtggga gcggctgggc agcgaggaga gccgggcgac ccggaacttc gccagcgcgc
     3661 ggcgggcctc gatgtcgtgg ccgtcgccga tggccaacgc gcgctacggc atgaccttcc
     3721 cgctcgccaa cgccggcgac ccgctgcccc gcaagttcgc ggtcgccacc accaagctgg
     3781 tccagctgtg ccgcagcacc cgcaacccgg gcaacgggct gcgcaacgcg ctgtcgacct
     3841 acctggaggc gtcgctggag agcgcgctcg agctgagccg cgagcaggag gcgttcggcg
     3901 agcacgcggt cgcggtcggc aacctgtgga ccgacgccga ggggctgctc cggccgtgct
     3961 tcgggttctt tccacccgac gcctgggtca cccagttcga ggccggccgc gggatcgccg
     4021 gccacgcgtt ccggttcgcc cggccggccg cgtggcaccg ctcgatcacc ggcgagttcg
     4081 acgtgatccg ggtgccgacg atgaccggca cgcgcgacta cgagtggatc ctctgcctgc
     4141 cgatcctgac cagccccgac ggcaccgcga tcggcgtgtt cgggttcgcc ggcacccgcg
     4201 atcacaacac cgagaccacc aaccagctcg cggcgttcgc ccagcagatc gcgcagcgcg
     4261 atccggcggt cgacaccgac accttcgacc ggttctggta cgtgctcaac gccagcttct
     4321 ggtacggcct ggccgacctc gcgggtgcgg cgcggctcga ctacggcgtg atcgacatgg
     4381 ccaaggagtg ctcgaccgcg ttcctcgcga ccgaccccga gtgatccgcc ggccggcgcg
     4441 gacacgcgcc cgcgcggccg cggatcagct catcgccgtc gtcccgcgtg tcgacgggcg
     4501 gcgggccggg acggcgggtc gagatgcccg acgatgcgcg gcgcggcacg agccgctcac
     4561 gtgacttcct gcgagtgggc gccttcgggt tccatggatc gtcatgccag gccatcgacg
     4621 gcgcagaggt gccccacgat ccgcccgtag tccaccggcg tgccgtccca tggcccggcg
     4681 atcgtatcgg cgtcgatctg tagc
//
LOCUS       DASVOR010001689         1676 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_279471,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001689 DASVOR010000000
VERSION     DASVOR010001689.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279471"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..959)
                     /locus_tag="VF516_31520"
     CDS             complement(<1..959)
                     /locus_tag="VF516_31520"
                     /inference="COORDINATES: protein motif:HMM:NF025055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pili methyl-accepting chemotaxis
                     transducer N-terminal domain-containing protein"
                     /protein_id="HEX8112319.1"
                     /translation="MRVSELTFRYVGALLIVAGLLVAGQTVIHQLLARQEGDARVINL
                     AGRQRMLGQRLSTLALALGVDTGDRARDTRDQLVRIADEWERIQAALWAGRPDTGLRG
                     SNSSAILELFAQIDPDHRAMLAAARAAAALPPGSQRPEYADAVRVHQDAFLAGMDRIV
                     AEYEREAKQRIVGLRRLELILLGLALLVLALVGAFVFRPAVSDLRSQLAERALVQHTL
                     DASEAERQAIQRQLLQIGDREQVRLAQDLHDGLGQHLIGVSFLLQPLRQRLAGDPAAA
                     QLDEVDRLVGEAIGQTRDLVRSLHSRTLEAAGLTAALGELAAQ"
     gene            1140..>1676
                     /locus_tag="VF516_31525"
     CDS             1140..>1676
                     /locus_tag="VF516_31525"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8112320.1"
                     /translation="MTAINAHHPESDVVPAALRGGVIEPQLAALRELQRKHPFWNCRL
                     LAGFAQGAFSRDDLRYIFSQYHLYTSSFTRFLAAVMVNCESDLFRSRLSENLWEEGVG
                     CDPERRHAQLFRNFLHRSLGVDDVTRIDYEPFTRQFVRDYLDQSLRYEPMAATAFLAI
                     GTEGIVPRVYEIMRGGLRQ"
BASE COUNT          248 a          586 c          599 g          243 t
ORIGIN      
        1 atctgcgccg cgagctcgcc gagcgcggcc gtcagcccgg cggcctccag cgtgcgcgag
       61 tgcaggctgc gcaccagatc gcgggtctgc ccgatcgcct cgccgaccag gcggtcgacc
      121 tcgtcgagct gggccgcggc cggatccccg gcgagccgct ggcgcagtgg ctggagcagg
      181 aacgacacac cgatcaggtg ctggccgagg ccgtcgtgga ggtcctgggc gaggcggacc
      241 tgctcgcgat cgccgatctg gagcagctgg cgctggatcg cctggcgctc ggcctcgctg
      301 gcgtcgagcg tgtgctggac cagcgcgcgc tcggcgagct gcgaccgcag gtcggacacc
      361 gccgggcgga acacgaacgc gccgaccagg gcgagcacga gcagcgcgag cccgagcagg
      421 atcagctcga gccggcgcag gccgacgatg cgctgcttgg cctcgcgctc gtactccgcg
      481 acgatgcgat ccatcccggc gaggaacgcg tcctggtgga cgcgcaccgc gtcggcgtac
      541 tcggggcgct gcgatccggg cggcagcgcg gccgcggcgc gggcggcggc gagcatcgcg
      601 cggtggtcgg gatcgatctg cgcgaacagc tcgaggatcg cggacgagtt gctgccgcgc
      661 aggccggtgt ccggccggcc ggcccacagc gcggcctgga tgcgctccca ctcgtcggcg
      721 atgcgcacca gctggtcgcg cgtgtcgcgc gcgcggtcgc cggtgtcgac gccgagcgcg
      781 agcgcgagcg tggacagccg ctggccgagc atgcgctggc gcccggcgag gttgatcacc
      841 cgcgcatcgc cttcctgacg cgcgagcagc tggtggatga cggtctggcc cgcgaccagc
      901 agccccgcca cgatcagcaa cgcgccgaca tagcggaacg tgagctcact tacgcgcatc
      961 gatggaggtc cacggagcgt acgcgatcaa gttccgcccc gacagcgctg tcatggtttc
     1021 ctcggcacga cgatcagggt gtagctgatt gctgcgcgga gtggcaaccc ggcaacgttc
     1081 gaagctgcgg cggttcgccc cgccgcagct tcgagagcaa gagccaaggg agcacactcg
     1141 tgacggccat caacgcacac caccccgaga gcgacgtcgt gcctgccgcg ttgcgcggcg
     1201 gggtcatcga gccccagctt gcggcgttgc gcgagctcca gcgcaagcac ccgttctgga
     1261 actgccggtt gctggcggga ttcgcccagg gcgcattttc ccgcgacgac ctgcgttaca
     1321 tattctcgca ataccatcta tacacgtcca gcttcacgcg gttcctcgcg gcggtgatgg
     1381 tcaactgcga gagcgatctg ttccggtccc gtctctcgga gaatctctgg gaagagggcg
     1441 tcggctgcga ccccgagcgg cgccacgccc agctgttccg gaacttcctg caccgctcgc
     1501 tgggcgtcga cgacgtgacg cggatcgact acgagccgtt cacccggcaa ttcgtgcgcg
     1561 attacctcga ccagtcgctg cgctacgagc cgatggcggc gaccgcgttc ctcgcgatcg
     1621 gcaccgaggg catcgtgccc cgggtctacg agatcatgcg tggcgggctc cgccag
//
LOCUS       DASVOR010001690         2553 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_134078,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001690 DASVOR010000000
VERSION     DASVOR010001690.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134078"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..158)
                     /locus_tag="VF516_31530"
     CDS             complement(33..158)
                     /locus_tag="VF516_31530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112321.1"
                     /translation="MTTWQKPNFTELSMNAEIGSYQEDFGGRGNVPPAPAKKKAR"
     gene            complement(420..563)
                     /locus_tag="VF516_31535"
     CDS             complement(420..563)
                     /locus_tag="VF516_31535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112322.1"
                     /translation="MSRSAASTAIAQTRTTAASAAGPFAADACTYTACAGAPGTAYTA
                     AGP"
     gene            735..1691
                     /locus_tag="VF516_31540"
     CDS             735..1691
                     /locus_tag="VF516_31540"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8112323.1"
                     /translation="MARQLLTLETAKVSWRDRVALWSDALTAVCGPLHTEPLGATLDG
                     TMTFGTLGRLQIGHIAVSRHRVGLTPALARAEHHPVAKVIVQTAGMSVYEQRGECVTL
                     TPGDGLVYDVSQPHAITSTETTEHFVVTVPHDLVAGRGIRLDRLSAQRFSARTGVGRL
                     AADLIHSTFAELATISPACEDDLAASLLGLMFLSLPSEAAHASEALRHRIEGYIRDQI
                     RDPELSIDKIAGALRCSKRYLHMAFAACDRSITDHIWATRLDGCRGDLARLSDRTISE
                     IAFAWGFSSSAHFSRAFRKRFGVTPTAFRRGDRGTSIPSRAG"
     gene            1779..2345
                     /gene="gfa"
                     /locus_tag="VF516_31545"
     CDS             1779..2345
                     /gene="gfa"
                     /locus_tag="VF516_31545"
                     /EC_number="4.4.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF003829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051907 - S-(hydroxymethyl)glutathione
                     synthase activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione synthase"
                     /protein_id="HEX8112324.1"
                     /translation="MSVGIHPSIDSGLKPGNSNFAGGTLSCRCTTKPVTVAISSGIAH
                     NHACGCTKCWKPAGATFSIVAVVPSDKVKVTANGDKLKVVDPSALIQRFACTACGAHL
                     YGPVEKEHAFKGLTFIHPELFTEAGAPPPEFAAFVSSVIEAGVDPAQMSAVRARIKEL
                     GLEPYDCLSPPLMDFLATYAAKQKGVRA"
     gene            2342..>2553
                     /locus_tag="VF516_31550"
     CDS             2342..>2553
                     /locus_tag="VF516_31550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013202337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8112325.1"
                     /translation="MKTRAAIAFEVGKPLVIEQVDLEGPKAGEVLVELKATGVCHTDE
                     FTRSGADPEGLFPVIFGHEGAGVVVEV"
BASE COUNT          369 a          872 c          916 g          396 t
ORIGIN      
        1 cggccgccga gccgagcacg cgcaggtaca aactagcgcg ccttcttctt cgccggtgcg
       61 ggcggcacgt tgccgcgacc gccaaaatcc tcctggtacg agccgatctc ggcgttcatg
      121 gacagttcgg tgaagttagg cttctgccac gtggtcattc ggtagcctcc tttgtgcggg
      181 tcatgatgca ctcgtgaggc ggtcagggcg agtggaaaag ccacattgtc tgcagatccg
      241 gagtcgggca tacgccgtcc ggcgcggcat cgtgtcgggc ggcgaccgat gatcgcggcg
      301 taagttgaat tacccacgcc gagcccgtgc gaagacggct gacgcgtcgc gatgtgtgac
      361 ctgcgcgccg gtgaccgccg cctgccgccc gggaccgcgc acgagatcgg ccgcacggtt
      421 cagggcccgg cggcggtgta cgcggtgccc ggtgccccgg cgcaggccgt gtacgtacat
      481 gcatcggcgg cgaacggtcc ggcggccgac gccgcggtcg tccgggtctg ggcgatcgcg
      541 gtcgatgcgg cggatcgcga cacccggcgc cccgtccggc cgcgggtatc gctgcgaccg
      601 cacgagcttt tcggcgggcg accgacggcg gcttgcggcc gtcgccgata tagcggatcg
      661 gcgtcccggg atgcctcggc gtcggcgggt gacgaagatt gacgatgcga gcgggctctg
      721 cgtaggctac gaccatggct cgccagctgc tcacgctcga gacagcgaag gtctcatggc
      781 gggaccgtgt cgcgctgtgg tccgacgcgc tgaccgccgt gtgtggcccg ctccataccg
      841 agccgctcgg agccacgctc gacgggacga tgacgttcgg gaccctcggg cggctccaga
      901 tcggccacat cgccgtgtcg cgccaccgcg tcgggctgac gccggcgctg gcgcgcgccg
      961 agcaccaccc cgtcgccaag gtcatcgtcc agaccgccgg tatgtcggtg tacgagcagc
     1021 gcggcgagtg cgtcacgctg acgcccggcg acgggctggt ctacgacgtc tcgcagccgc
     1081 acgcgatcac cagcaccgag accaccgagc acttcgtcgt caccgtcccg cacgacctgg
     1141 tggcggggcg cgggatccgg ctcgaccggc tgagcgcgca gcggttctcc gcgcgcaccg
     1201 gggtcggccg gctcgccgcg gatctgatcc actcgacgtt cgccgagctg gccacgatct
     1261 cgccggcctg cgaggacgac ctcgcggcgt cgctgctcgg cctgatgttc ctgtcgctgc
     1321 cgagcgaggc ggcgcacgcg agcgaggccc tgcgccaccg gatcgagggc tacatccggg
     1381 atcagatccg cgaccccgag ctgtcgatcg acaagatcgc cggtgcgctg cggtgcagca
     1441 agcgctacct ccacatggcc tttgcggcgt gcgaccgctc gatcacggac cacatctggg
     1501 cgacgcggct cgacggctgt cgcggcgatc tcgcgcgcct gtccgatcgg acgatctccg
     1561 agatcgcgtt cgcctggggg ttctcgagct cggcgcactt cagccgggca tttcgcaagc
     1621 ggttcggggt gaccccgacg gcgtttcgcc gcggcgaccg cggcacgtcg atcccgtctc
     1681 gggcgggctg agcgttcgct cgcggtcaac ccgcagtgcg cggtgagaca agccaaacgc
     1741 ggccgggccg cggcaatgtt ccgcagcgag gaggccacat gtccgttggg atccatccgt
     1801 ccatcgattc ggggctgaag ccgggcaaca gcaactttgc cggtggcacg ctgtcgtgtc
     1861 gctgcacgac gaagcccgtg acggtcgcga tcagctcggg gatcgcgcac aaccacgcgt
     1921 gcggctgcac gaagtgctgg aagccggccg gcgcgacgtt ctcgatcgtc gcggtcgtgc
     1981 cgagcgacaa ggtcaaggtg accgcgaacg gcgacaagct caaggtcgtc gacccgtcgg
     2041 cgctgatcca gcgcttcgcg tgcaccgcct gcggcgcgca cctgtacggg ccggtcgaga
     2101 aggagcacgc gttcaagggg ttgaccttca tccatcccga gctgttcacc gaggccggcg
     2161 cgccgccgcc cgagttcgcg gcgttcgtgt cgtcggtgat cgaggccggc gtcgatcccg
     2221 cgcagatgtc ggcggtccgc gcccggatca aggagctcgg gctcgagccg tatgactgtc
     2281 tgagcccgcc gctgatggac ttcctggcca cctacgcggc caagcagaag ggagtccggg
     2341 catgaagacc cgcgccgcga tcgcgttcga ggttggcaag ccactggtca tcgagcaggt
     2401 cgacctcgag ggccccaagg ccggcgaggt gctggtcgag ctcaaggcga ccggcgtgtg
     2461 ccacaccgac gagttcacgc ggtcgggcgc cgatcccgaa ggcctgttcc cggtgatctt
     2521 cggccacgag ggcgccgggg tggtggtgga ggt
//
LOCUS       DASVOR010001691         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_396578,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001691 DASVOR010000000
VERSION     DASVOR010001691.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396578"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VF516_31555"
     CDS             <1..654
                     /locus_tag="VF516_31555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112326.1"
                     /translation="PGMGPPGMPNPQGMGPPAMPMGGPPAMPLGGPPAMPMGGPPGVR
                     PAANDAKTMIAQPSPFAQGGMTPGGPAPGGMPPGSPYGMPASGPPGPSMAPGGAPGPG
                     GYSPASPGQKTIVAGMAPAIMGGQMMPPGQMPASIGYPAGASPQMPPGGAPGPGPNKT
                     VMLAPSEGVVSVARSGHAVPPVGSGAIQQGASTLFWIVSLVIGVAVGALAYVIVLQL"
     gene            672..1364
                     /locus_tag="VF516_31560"
     CDS             672..1364
                     /locus_tag="VF516_31560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112327.1"
                     /translation="MPDPNDNDDNQADQQVDPKVDPRVKELIDSATRAELERWFGLPS
                     YEALADQGLRPEPPADEDPKLAERRKRQDAALAAIDPALVEAHRRRTEGMASILKPPP
                     EVTLHIDPSIVQLDTVMIERQGAIADPRELAQPPDVVDALTERTPQALLRDLHRPELD
                     FDKVFQRVDMIEEYRVDIAASIEEVMATSTRLPPPETPPFKECYALLHELRAERKQPW
                     IHIEMPRRRVTE"
     gene            1426..>2132
                     /locus_tag="VF516_31565"
     CDS             1426..>2132
                     /locus_tag="VF516_31565"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX8112328.1"
                     /translation="MNAESRTVPVERFLLRAVGRTDVGAQRTQNEDAMYYDDFLGVYI
                     VCDGMGGHASGQVASDIAIRTIVHSLKTGDPLPAPGQDQLVAAMKAANAAVFQRSLTD
                     PTCHGMGTTAVGLRFEENIVHVCHCGDSRAYLLRQGQLQQITRDHSLRNLYQDRPDLI
                     GTLGPATSNVIIRAVGLDASVEIEHNTMAPEHNDIFLLCCDGLSDLVDDWMIREIMTS
                     GDSLEVVAENLIRAANN"
BASE COUNT          339 a          760 c          742 g          291 t
ORIGIN      
        1 ccgggcatgg ggccgccggg catgccgaac ccgcagggca tgggcccccc ggcgatgccg
       61 atgggcggtc caccggcgat gccgctgggc ggcccgcccg ccatgccgat gggcggcccg
      121 cctggtgtgc gccccgcggc caacgatgcc aagacgatga tcgcccagcc cagtccattc
      181 gcacagggcg gcatgactcc tggcggcccg gctcctggcg gcatgcctcc tgggtctccc
      241 tacggcatgc ctgcgagcgg tccacccggg ccgtcgatgg cgcccggcgg tgcgccgggg
      301 cccgggggat acagcccggc gtcgccgggg cagaagacca tcgtcgccgg gatggcaccg
      361 gcgatcatgg gcggccagat gatgccgcct ggccagatgc ccgcgtcgat cgggtacccg
      421 gctggcgcgt cgccgcagat gccgccgggg ggcgcccctg gcccaggccc gaacaagacc
      481 gtgatgctgg cgcccagcga aggtgtggtg tcggtcgcac gctcggggca tgctgtcccg
      541 cccgtgggca gcggggcgat ccagcagggc gcttccacgc tattctggat cgtgagcctg
      601 gtcatcggag ttgccgtcgg cgcactcgcg tacgtcatcg tcctccagct ctaggcgcgc
      661 ggtgcccggc gatgcccgac cccaacgaca acgacgacaa ccaggccgat cagcaggtcg
      721 atccgaaggt cgatccgaga gtcaaagagc tgatcgatag cgccacgcgc gccgagctcg
      781 agcgctggtt cggcctgccg tcgtacgagg cgctggccga tcagggcctt cgccccgagc
      841 cgccggccga cgaggatccc aagctcgccg agcgccgcaa gcggcaggac gccgcgctcg
      901 ccgcgatcga tccggcgctg gtcgaggccc accgccgccg gaccgagggg atggccagca
      961 tcctcaagcc accgcccgag gtcacgctgc acatcgatcc cagcatcgtg cagctcgaca
     1021 cggtgatgat cgagcgccag ggcgcgatcg ccgatccccg cgagctcgca cagccgcccg
     1081 acgtcgtgga tgcgttgacc gagcgcacgc cgcaggccct gctccgcgac ctgcaccgcc
     1141 ccgagctcga cttcgacaag gtgttccagc gcgtcgacat gatcgaagag taccgggtcg
     1201 acatcgcagc cagcatcgag gaggtcatgg cgacgtcgac ccggctgccg ccgcccgaga
     1261 cgccgccgtt caaggagtgc tacgcgctgc tgcacgagct gcgcgccgag cgcaagcagc
     1321 cctggatcca catcgagatg ccgcgcaggc gggtgacgga gtgaggaagg tgtgctggga
     1381 tgagggcgta gatcgcatga tggtcgtgcg cccggaggtg ctcgcgtgaa cgcggagtcg
     1441 cgcacggttc ccgtcgagcg cttcctgctg cgcgcggtcg gccgcaccga tgtcggagcg
     1501 cagcgcaccc agaacgagga cgcgatgtac tacgacgatt tcctcggcgt gtacatcgtg
     1561 tgcgacggca tgggtgggca tgcgtccggg caggtggcgt ccgacatcgc gatccgcacg
     1621 atcgtgcact cgctcaagac cggcgatccg ctgccagcgc ccgggcagga ccagctggtg
     1681 gccgcgatga aggccgccaa cgcggcggtg ttccagcgct cgctcaccga tccgacctgc
     1741 cacggcatgg gcaccaccgc ggtcgggctc cggttcgagg agaacatcgt ccacgtctgc
     1801 cactgcggcg actcgcgggc gtacctgctg cgccagggcc agctccagca gatcacgcgc
     1861 gatcactcgc tgcgcaacct ctaccaggac cggcccgacc tgatcggcac gctcggcccg
     1921 gcgacgtcga acgtgatcat ccgcgcggtc gggctcgacg cctcggtcga gatcgagcac
     1981 aacaccatgg cgccggagca caacgacatc ttcctgctgt gctgcgacgg cctgtcggat
     2041 ctggtcgacg actggatgat ccgggagatc atgacctcgg gcgactcgct ggaggtcgtc
     2101 gccgagaacc tgatccgcgc cgccaacaac aa
//
LOCUS       DASVOR010001692         1682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_222003,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001692 DASVOR010000000
VERSION     DASVOR010001692.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1682
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222003"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VF516_31570"
     CDS             <1..758
                     /locus_tag="VF516_31570"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEX8112329.1"
                     /translation="FAHPDPGAVARGLVPDPGILAEPDRLYLAIGIIGATVMPHNLYL
                     HSSIVQTRRYPLDLKGRTAAVRLATVDLVAALLVAVVVNGAIVVLAAAAFHDTGHRWV
                     AELGDAYRVLSPVLGAGAASVLFGIALLASGKSSTLTATLTGQIVMEGFLSLRLSPWL
                     RRLITRGLAILPALIVVAVCGEHGLAWLLIASQAVLSFQLPFAVFPLIRLTSDRRRMG
                     KLVNPVWLTAAAWLSGLAIAGLNTWLLVQLVIG"
     gene            complement(794..>1682)
                     /locus_tag="VF516_31575"
     CDS             complement(794..>1682)
                     /locus_tag="VF516_31575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112330.1"
                     /translation="GVPFEVAVLPGSELRARFPDGPAERRSMHLLGKLRMTHRAIPSQ
                     AVVVIVLYSDVAADYFVETMAADVAADIDRTYGDGVALADAQAGIPVVLKEVATAAGL
                     GTIGKNALFFSHRFGFNCKLSAVLLDAPVSRYEPTPRGADWKLPDCATCNLCVEACPV
                     GAFDDYAIHDVAACDRFISSDYFGPRRDHMCRACITRCPVSSDLLKLRRREGAPQRTF
                     WDNEAQLSLVADLFMYRPSFWVWVMQRFYYGADLPGRPGDRKKGFAESLASSVNVATA
                     DRTRDGWRLWARKRSREPR"
BASE COUNT          216 a          609 c          603 g          254 t
ORIGIN      
        1 tgttcgccca ccccgatccc ggcgcggtcg cccgcggcct cgtgcccgat cccgggatcc
       61 tcgccgagcc ggaccggctc tacctcgcga tcgggatcat cggcgcgacg gtgatgccgc
      121 acaacctcta cctgcactcg tcgatcgtcc agacccggcg ctatccgctc gacctcaagg
      181 gccggaccgc cgcggtccgg ctggcgaccg tcgatctggt ggccgcgctc ctggtggccg
      241 tcgtggtcaa cggcgccatc gtggtgctcg cggccgcggc gttccacgac accggccacc
      301 gctgggtcgc ggagctcggc gacgcgtacc gcgtgctgtc gcccgtgctc ggcgccggcg
      361 ccgccagcgt gctgttcggg atcgcgctgc tggcgtcggg caagagctcg acgctgaccg
      421 ccacgctcac cgggcagatc gtgatggagg ggttcctgtc gctgcggctg tcgccgtggc
      481 tgcgccggct gatcacccgc gggctcgcga tcctgcccgc gctgatcgtg gtcgccgtgt
      541 gcggcgagca cgggctggcc tggctgctga tcgccagtca ggcggtgctc agcttccagc
      601 tgccgttcgc cgtgttcccg ctgatccggc tgaccagcga ccgccgccgg atgggcaagc
      661 tggtcaatcc ggtctggctg accgcggctg catggctgtc cggactggcg atcgccgggc
      721 tcaatacctg gctgctggtc cagctcgtca tcggctgatg cggcaggtgg cggctcgacc
      781 gctgacggcc gcgctaccgc ggctcgcgcg agcgcttgcg cgcccacagc cgccagccgt
      841 cgcgcgtccg gtcggcggtc gcgacgttca ccgacgacgc cagggactcg gcgaagccct
      901 tcttcctgtc gcccggccgg cccggcagat cggcgccgta gtagaagcgc tgcatcaccc
      961 acacccagaa cgacggccgg tacatgaaca ggtcggcgac cagcgacagc tgggcctcgt
     1021 tgtcccagaa cgtgcgctgc ggggcgccct cgcgccgccg gagcttcagc agatcgctgg
     1081 acaccgggca gcgcgtgatg cacgcgcggc acatgtgatc gcgccgcggg ccgaagtagt
     1141 cgctcgagat gaagcggtcg cacgcggcga cgtcgtggat cgcgtaatcg tcgaacgcgc
     1201 cgaccgggca ggcctcgacg cacaggttgc aggtcgcgca gtccggcagc ttccagtcgg
     1261 cgccgcgcgg cgtcggctcg tagcggctga ccggcgcgtc gagcagcacg gcgctgagct
     1321 tgcagttgaa gccgaagcgg tgggagaaga acagcgcgtt cttgccgatc gtgcccagcc
     1381 ccgcggcggt cgcgacctcc ttgagcacga ccgggatgcc ggcctgggcg tcggccagcg
     1441 cgacgccgtc gccgtacgtg cggtcgatgt cggccgcgac gtcggcggcc atggtctcga
     1501 cgaagtagtc ggcggccacg tcgctgtaca ggacgatcac gaccaccgcc tggctgggga
     1561 tcgcgcggtg agtcatccgc agcttgccga gcaggtgcat gctgcggcgc tcggccggtc
     1621 cgtcggggaa ccgcgcgcgc agctcgctgc cgggcagcac cgcgacctcg aacggcacac
     1681 cg
//
LOCUS       DASVOR010001693         3405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_338462,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001693 DASVOR010000000
VERSION     DASVOR010001693.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3405
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338462"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1433)
                     /locus_tag="VF516_31580"
     CDS             complement(276..1433)
                     /locus_tag="VF516_31580"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8112331.1"
                     /translation="MLDFGRYVAGPWCAALLGDLGAEVIRIEKRGGSEDRFVGPVADG
                     GEGALFLQVNRNKKSMTLDPAAPAGREVVRRLVATADVVVANLPPQRLRALGLDWDTV
                     SKLRPSLVLTTISAFGRGGPYEERLGFDGIGQAMSGAMYLSGHPGEPMKAYVPWVDFG
                     TAGLAAFATVAALYERRTTGLGRHVEASLLHTALAAANAPLIEQQVRRLDRVATGNRS
                     QTTGPSDAFRTADGWVLVQVVGQPLFARFAALIGEPALLEDPRFADDSARGAHGAELS
                     ERMARWCAARTTAEVLAALERAQIPAGPVHSPAQALADPHIDAAGMLRALDYPGLPTP
                     APVASTPVQFARTAVDELVRAPLLGEHTDEILRELGYDDAAIAALRADEVV"
     gene            1519..3375
                     /locus_tag="VF516_31585"
     CDS             1519..3375
                     /locus_tag="VF516_31585"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8112332.1"
                     /translation="MACRDDSRDDRVRDIVARWPVRRQEYSSMGNDRVNRTRGHTEPG
                     AAPPGAGTTGVAPGKQTLVEATASQELPPALRGRFEQSLGADLGGVRVHTGADSAAAA
                     SGLDARAFAAGRDIHFAGGQYRPDDAAGQHLIAHEVAHTVQQARAGQATQPKLDVSSP
                     GDAAEVEADRAADAMVSGRSFQVTATPAHAIHRNTGAPPPAPAPEVDLDAELAGDKPD
                     YPKVLAALNQLNMLAMVQRLDKLRAQLPRLLGHIEGDANPGVTRVRAAVRAVQIKGTD
                     TSTEPGGQKLQQVMDELTAAKVAVWERVDIAVYLEGSAGVMRKKVLDDLAAQIDQAIL
                     KVKEVELDKPSAEKGTALETQRNDEAALRDGALRLVHDYMRLVDAELAVLADPALTTR
                     RKEKERFDRFKMMRESEALDDKMLHAASAADYKAAKARKYELDKLLGKDGPDRKAVGT
                     TGAHAGKTYVVYEDTVKLGGDLAWLNNNPGNMIASPLATNFKNQTFAIFPTMALGYAA
                     IPMQFKEWRMEFPGVYTLLKTFQRWANRPGDNPEAYAASVAKGLHAQGVTAKGAPITS
                     ATLLDDLDDSGLILAGEVMGQSVEGMKPGTTYHRSDMASEKWLRDLLGPPYP"
BASE COUNT          523 a         1237 c         1237 g          408 t
ORIGIN      
        1 gcaggcgtcg ccgtggtcgc cggcgtcgtg ccgccggtcg ccggggtcgg cgacgacgcc
       61 gcgccggtca gcgtggtctt gccgacgggc ccgatgtctg ccgtggcggc gacggaatcg
      121 gtgaggctgg taccaccggg cttcgtctcg aaggcggcca tcgcatgtca gtaacacacc
      181 gcgtcgctcc ttgtcgacgg acagccccgg agcggccggc ggcggtgcgg gcggcgtcac
      241 acgcgctggt cgcggcgccg ctacagcttc ttcacctaca cgacctcgtc ggcgcgcagc
      301 gcggcgatcg cggcgtcgtc gtagccgagc tcgcgcagga tctcgtcggt gtgctcgccg
      361 agcagcggcg cgcgcacgag ctcgtccacg gcggtgcgcg cgaactgcac gggagtgctc
      421 gcgaccgggg cgggggtcgg cagcccggga tagtcgagcg cgcgcagcat gccggccgcg
      481 tcgatgtgag ggtccgcgag tgcctgggcc ggcgaatgaa ccgggccggc ggggatctgc
      541 gcacgctcga gcgccgcgag gacctcggcc gtcgtgcgcg ccgcgcacca gcgcgccatg
      601 cgctccgaga gctccgcccc gtgggcgccg cgggcgctgt cgtcggcgaa ccgcggatcc
      661 tcgagcagcg cgggctctcc gatcagcgcc gcgaagcgcg cgaacagcgg ctgcccgacc
      721 acctgcacga gcacccagcc gtcggccgtc cggaacgcat cgctcggccc ggtggtctgc
      781 gagcggttgc cggtcgcgac ccgatcgagc ctgcgcacct gctgctcgat cagcggcgcg
      841 ttcgccgcgg cgagcgcggt gtgcagcagc gacgcctcga cgtgccgtcc gagcccggtg
      901 gtgcgccgct cgtacagcgc ggccacggtc gcgaacgcgg cgagcccggc ggtgccgaag
      961 tccacccacg gcacgtacgc cttcatcggc tcgccggggt ggcccgagag gtacatcgcg
     1021 cccgacatcg cctgaccgat gccgtcgaag ccgagccgct cctcgtaggg cccgccgcga
     1081 ccgaacgccg agatcgtcgt gagcaccagc gacggccgga gcttggagac ggtgtcccag
     1141 tcgagaccca gcgcgcgcag ccgctgcggc ggcaggttcg cgaccacgac gtcggccgtc
     1201 gcgaccagcc gccgcaccac ctcgcggccg gcgggcgcgg ccgggtcgag cgtcatgctc
     1261 ttcttgttgc ggttgacctg caggaacagc gcgccctcgc cgccgtccgc gaccggcccg
     1321 acgaagcggt cctcgctgcc gccgcgcttc tcgatgcgaa tcacctcggc gccgaggtcg
     1381 ccgagcagcg ccgcgcacca cgggcccgcg acgtagcggc cgaagtcgag cactcggatt
     1441 ccatggaaga tctccatcgc ctggatggcg gcatgatagt cgcggcgagc tcgggcgcgc
     1501 catgtgacgg tggcaatgtt ggcctgccgt gatgactccc gcgacgaccg cgtccgcgat
     1561 atcgtggcgc gctggccggt gcgccggcag gagtacagca gcatggggaa cgaccgggtc
     1621 aaccggacgc gcggacacac ggagccgggt gcggcaccac cgggagcggg gaccacggga
     1681 gtcgcccccg ggaagcagac gctggtcgag gcgaccgcgt cgcaggagct gccaccggcg
     1741 ctgcgcggcc ggttcgagca gtcgctcggc gcggacctcg gtggggtccg cgtccacacc
     1801 ggcgcggatt cggcggccgc ggcgtcgggg ctggacgcgc gggcgttcgc cgccggacgc
     1861 gacatccact tcgccggtgg gcagtaccgg ccggacgacg ccgccggcca gcacctgatc
     1921 gcgcacgagg tcgcacacac ggtgcagcag gcgcgcgccg gccaggcgac ccagcccaag
     1981 ctcgacgtgt cctcgccggg cgacgcggcc gaggtcgagg ccgatcgcgc cgccgacgcc
     2041 atggtgtcgg ggcgctcgtt ccaggtcacg gcgacgcctg cgcacgcgat ccaccgcaac
     2101 accggcgccc cgccgcccgc gccggcacca gaggtcgatc tcgacgcgga gctcgcaggc
     2161 gacaagcccg actatcccaa ggtgctcgcc gcgctcaacc agctcaacat gctcgcgatg
     2221 gtgcagcggc tcgacaagct gcgcgcgcag ctgcccaggc tgctggggca catcgagggc
     2281 gacgccaacc ccggcgtcac ccgggtccgc gccgccgtgc gcgccgtcca gatcaagggc
     2341 accgacacca gcaccgagcc gggcggccag aagctgcagc aggtcatgga cgagctcacc
     2401 gccgccaagg tcgcggtgtg ggagcgggtc gacatcgcgg tctacctcga gggcagcgcc
     2461 ggggtgatgc gcaagaaggt gctcgacgat ctcgccgccc agatcgacca ggcgatcctg
     2521 aaggtcaagg aggtcgagct cgacaagccg agcgccgaga agggcaccgc gctcgagacc
     2581 cagcgcaacg acgaggccgc cctgcgcgac ggggcgctgc gcctggtcca cgactacatg
     2641 cgcctggtcg acgccgagct cgccgtgctc gccgaccccg cgctgaccac gcgccgcaag
     2701 gagaaggagc gcttcgatcg cttcaagatg atgcgcgagt ccgaggcgct cgacgacaag
     2761 atgctgcacg ccgccagcgc cgccgactac aaggccgcca aggcccgcaa gtacgagctc
     2821 gacaagctgc tcggcaagga cggcccggac cgcaaggcgg tcggcaccac gggcgcccac
     2881 gccggcaaga cctacgtggt ctacgaggac accgtcaagc tcggcggcga tctggcctgg
     2941 ctcaacaaca acccgggcaa catgatcgcc agcccgctcg cgaccaactt caagaaccag
     3001 acgttcgcga tcttcccgac gatggcgctc ggctacgcgg cgatcccgat gcagttcaag
     3061 gagtggcgga tggagttccc gggcgtgtac acgctgctga agacgttcca gcgctgggcg
     3121 aaccggccgg gcgataaccc ggaggcctac gcggccagcg tcgcgaaggg cctccacgcc
     3181 cagggggtca ccgccaaggg tgccccgatc acctcggcga cgctgctcga cgacctcgac
     3241 gacagcgggc tgatcctggc gggcgaggtg atgggtcaga gcgtcgaggg catgaagccg
     3301 gggaccacct atcaccgctc cgacatggcc agcgagaagt ggctccggga tctgctcggg
     3361 ccgccgtatc cgtgagccct ggcatgatcc gcacggggtc gcggg
//
LOCUS       DASVOR010001694         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_222019,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001694 DASVOR010000000
VERSION     DASVOR010001694.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222019"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VF516_31590"
     CDS             <1..1572
                     /locus_tag="VF516_31590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8112333.1"
                     /translation="IHAAAIAEGPGPLGGSWLVGVRRSYFDAIEDAAGLDLALAPRYG
                     DAQLRWESGDGRWMAILFGADDLLRLLHDPNDASTGGINTSNVKSFEYSSRFARLGVR
                     YRAVFGATQLTITPSAGIDDISAHADQNDLDKGLHRTTVPLALRADLATPLAGGTLLV
                     GLDGGGSRYSYSMLNTPPPNPTNPAPTMVIQRDLALWAADLGAFIEQSWFLAGDAIEL
                     RPGLRGDHFGLSDQWTLDPRLAVHERLPYGIVLTQQVGVYHEPPLITDLDPVFMRKTP
                     MLGSKAIQYAVGAKAIIADSGEIGATAYYQDLSQLPVDAVSAATPISANGAEESGGLL
                     GISRELVDTQFGSYSYREAIGTGHAYGIELIARRNTGRWTGWIAYTYSRSWRRDPVRS
                     TTDLPYVLDQPHSLTVLATTALGDSWRLGGRFRYTTGNPFTPVDHAMMVGSQYVAIDG
                     PLLSERLPAFVQLDLRLDHAWRRRWGTLDLYIDVQNVSNRDNAEGVTYNKDYSQRSYT
                     TGLPIFPAIGVEYIP"
     gene            1604..1852
                     /locus_tag="VF516_31595"
     CDS             1604..1852
                     /locus_tag="VF516_31595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112334.1"
                     /translation="MKLALALLVLAACGGSMAKPESPIVNEGSDTPETCCCKSFPLTS
                     EDGKPVFEMAPRMECSARQGTCVNDVQCHVESGAGSGH"
     gene            complement(1954..>2164)
                     /locus_tag="VF516_31600"
     CDS             complement(1954..>2164)
                     /locus_tag="VF516_31600"
                     /inference="COORDINATES: protein motif:HMM:NF036850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112335.1"
                     /translation="ALRAGAWVATLVYHRRTRLLDRAAAHGHPTLDGRGMLVHQGARA
                     FAIFTGAPPPVAAMQRALDAALAGA"
BASE COUNT          320 a          829 c          728 g          287 t
ORIGIN      
        1 atccacgccg cggcgatcgc cgagggcccc gggccgctcg gcggcagctg gctggtcggc
       61 gtccggcggt cgtacttcga cgcgatcgag gacgccgccg gcctcgacct cgcgctcgcg
      121 ccgcgctacg gcgatgccca gctgcgctgg gagtcgggcg acggccgctg gatggcgatc
      181 ctgttcggcg ccgacgacct gctccggctg ctccacgatc ccaacgacgc ctcgaccggc
      241 gggatcaaca ccagcaacgt caagagcttc gagtacagct cgcggttcgc ccggctcggc
      301 gtgcgctacc gcgcggtgtt cggcgcgacc cagctgacga tcacgccgtc ggccggcatc
      361 gacgacatct ccgcgcacgc cgaccagaac gacctcgaca agggcctgca ccgcacgacc
      421 gtcccgctcg ccctgcgcgc cgacctcgcg accccgctcg ccggcggcac gctgctggtc
      481 ggcctcgacg gcggcgggtc gcgctactcg tacagcatgc tcaacacgcc gccgcccaac
      541 ccgacgaacc cggcgccgac catggtcatc cagcgcgacc tggcgctgtg ggcggcggac
      601 ctcggcgcgt tcatcgagca gtcgtggttc ctcgccggcg acgcgatcga gctccggccc
      661 ggcctgcgcg gcgatcactt cggcctgtcc gatcagtgga cactcgatcc gcggctcgcg
      721 gtgcacgagc ggctgccgta cggcatcgtg ctgacccagc aggtcggcgt gtaccacgag
      781 ccgccgctga tcaccgacct cgacccggtg ttcatgcgca agaccccgat gctcggctcg
      841 aaggcgatcc agtacgccgt gggcgccaag gcgatcatcg ccgacagcgg cgagatcggc
      901 gcgaccgcct actaccagga cctgtcgcag ctgccggtcg acgcggtcag cgccgcgacc
      961 ccgatctcgg ccaacggcgc cgaggagtcg ggcgggctgc tcgggatcag ccgcgagctg
     1021 gtcgacaccc agttcggctc gtacagctac cgcgaggcga tcggcaccgg ccacgcctac
     1081 ggcatcgagc tgatcgcgcg ccgcaacacc ggccggtgga ccggctggat cgcctacacc
     1141 tacagccggt cgtggcgccg cgatccggtg cgcagcacga ccgatctgcc ctacgtgctc
     1201 gaccagcccc actcgctgac cgtgctcgcc accaccgcgc tcggcgacag ctggcggctc
     1261 ggcggccggt tccgctacac caccggcaac ccgttcacgc cggtcgatca cgcgatgatg
     1321 gtcggtagcc agtacgtcgc gatcgacggc ccgctcctgt ccgagcggct gcccgcgttc
     1381 gtccagctcg acctgcggct cgaccacgcg tggcggcgcc gctggggcac gctcgacctc
     1441 tacatcgacg tccagaacgt ctccaaccgc gacaacgccg agggcgtcac ctacaacaag
     1501 gactacagcc agcgcagcta caccaccggc ctgccgatct tccccgcgat cggcgtcgag
     1561 tacatcccgt agcgccccgg ccgactcgtg ttaccaccgg gccatgaagc tagcgctcgc
     1621 cctgctggtg ctcgccgcgt gcggcggatc gatggccaag ccggagtcgc cgatcgtcaa
     1681 cgagggctcg gacacccccg agacctgctg ctgcaagtcg ttcccgctga ccagcgagga
     1741 cggcaagccg gtgttcgaga tggcgccgcg gatggagtgc tcggcgcgcc agggcacgtg
     1801 cgtcaacgac gtgcagtgcc acgtcgagtc cggcgccggc tccggccact gactaccagg
     1861 tcacccacat ctcgtcgaga agcgcgtcgc agcgtccggt gtcggcaccg atcgtggagt
     1921 tgcgatccgc gctgcaaccc ggccgcaaaa gcgtcaggcg cccgcgagcg ccgcgtcgag
     1981 ggcgcgctgc atcgccgcga ccggcggcgg cgcgccggtg aagatcgcga acgcccgcgc
     2041 gccctggtgg accagcatgc cgcgaccgtc gagcgtgggg tggccgtgcg ccgcggcgcg
     2101 gtcgagcagg cgcgtgcggc ggtggtagac cagcgtcgcg acccaggcgc cggcgcgcag
     2161 cgcc
//
LOCUS       DASVOR010001695        14111 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_279513,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001695 DASVOR010000000
VERSION     DASVOR010001695.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14111
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279513"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VF516_31605"
     CDS             complement(<1..450)
                     /locus_tag="VF516_31605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112336.1"
                     /translation="MRAWIVVAIVGGAACGAAHPHASSDQAALRAAHEAVLAHHRARD
                     WKALAPNEAMLDIGNGELSRITPEMGRARFQRYLERAEFSRYEDLVAPIVRVSADGTV
                     GWVIAQVRVEGTYREDDGVRTPLDTTWAWVELYEHAAGGWRMTGIAST"
     gene            964..1128
                     /locus_tag="VF516_31610"
     CDS             964..1128
                     /locus_tag="VF516_31610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112337.1"
                     /translation="MERSGPQRAAAEPLQDVADPVAAAIEAALALRRSGADAKVLRRA
                     LRRIEELLDE"
     gene            1386..2138
                     /locus_tag="VF516_31615"
     CDS             1386..2138
                     /locus_tag="VF516_31615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112338.1"
                     /translation="MVVWEISVHISVTFADPQVASKRVAYVGVHPIPAAEGGGFCNIE
                     GPHVHIYRPAHAEELYRVIHGRYLFVGDPVPFGYEGPRHAYSGRHPVPVDELVPGEHP
                     EHDGAVFCYLDGPHYHIYAPPPGLAFVLRGDAYWYLGGWPRSYQLDGSRYQKINVIYA
                     KLTYARPVVTAAAPAGYRVPVIEIATPRPAPVIVHAPPVVKVRARPGLIVDGDDDDDD
                     DDGVYYYYRRQPHHHHHHHHHHHGVEVIEIVP"
     gene            complement(2168..3631)
                     /locus_tag="VF516_31620"
     CDS             complement(2168..3631)
                     /locus_tag="VF516_31620"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8112339.1"
                     /translation="MSAIASGIVRSTSLGTPRHLISSVRRNGPDTPDTIMAMGSEHRP
                     ATTLLQIGGRATAIQVRGFALEFLSGPDAGRSIRGDKRSLLFGTHASADVVLADRHAS
                     RLHARLDVEDAEYVLRDLGSTNGTRVGGTRVRDACLEDGALIELGATRLRFRLLDEPF
                     EVRLAEDDQFEGLIGRSVAMRELFAVCARVAPTDAPVIIEGETGTGKELVARAIHRRS
                     LRAGKPLVVLDCSAIPPSLVESELFGHERGAFTGAVATRPGVFERADSGTVFLDELGE
                     LPIELQPKLLRCLESGEFTRVGGDRTIRVDIRVIAATNRDLPRMVTENRFRADLYYRL
                     AVIRVTVPALRDRRDDIPLLAAHFAAAALGNRARPPLPPQMLEALFGELTRHDWPGNV
                     RELRNAVERAAILADPKIIHAGVIDQAAIELQRSVEKAIHKQGTLRAARAEREREYLA
                     DLLRATEGNLDEAARIAQVHRKSLERLIRRHKLRDAR"
     gene            3609..4520
                     /locus_tag="VF516_31625"
     CDS             3609..4520
                     /locus_tag="VF516_31625"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112340.1"
                     /translation="MPLAIADIAGPLLAGRYRLRALLGRGGTGEVYEAEDIRTGAALA
                     IKKLAPGAGDATRCAERFRREAEAAGLLRHPNIVEVLDFVSEQDSLYLVMELVRGRSV
                     GALIDSGELTARRTLVIARQVLEALAHAHARGMIHRDIKPDNLMLVSAGEPRREYERV
                     KLLDFGLVKWIGDPAGGLGGEQLTQTGAVFGTPAYMAPEQALNRPLDERVDLYALGVV
                     VFEMLTGRPPFRSPDPMTLLRMQASAPPPALSAIALGQPWCTPALEQLVMRALAKQPA
                     ARFASALEMMAALEVAFLSLDHLPAGG"
     gene            5258..5467
                     /locus_tag="VF516_31630"
     CDS             5258..5467
                     /locus_tag="VF516_31630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112341.1"
                     /translation="MESRALRLVDGTTNAVERSGPQRDAAEPLQDVADPVAVALESAL
                     ALRRSGADAKALRRVLRRIEELLDE"
     gene            5504..5965
                     /locus_tag="VF516_31635"
     CDS             5504..5965
                     /locus_tag="VF516_31635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112342.1"
                     /translation="MLDAAVVALAAAISKQLGTGVLPRGGGAVDHEERALVAARVLGG
                     LAGDVAQELPRVGGGGGGVTVPARRSRWRYHQVPGAPATGCARLVDMLDLVGGLSFPG
                     NPVASLASCTARAGEERAQRKIGRAIARDRRTLVSEPSSSKSLRRERCRLR"
     gene            6013..8148
                     /locus_tag="VF516_31640"
     CDS             6013..8148
                     /locus_tag="VF516_31640"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8112343.1"
                     /translation="MKGIPGHASVGIDGAAVQRKAQGEAAPDGASPTETAGSVGAAGD
                     DGAVHRVAAAGVSGTGERLPHLERIQNAFGPDHDMSGIKAHVGSAAGEAAAQIGARAY
                     ATGNNVAFAGEPDVHTAAHEAAHVVQQRGGVQLKGGVGAAGDAHEVHADAVADRVVRG
                     ESAAELLAPYRGGETMTGGVQRAPASPGSAADIAAPTGGINLPGFIDTSDGANLRTGP
                     AEAGGKTVIDAPLPPATRVFVSGTYPSAPEWWYVTAFLPDKIVRGYVQQFRVNTDLPE
                     PLAKLHQVKSGDTVEKLAVQEYASSVRDGHDLRYYENVLLFANKQRGRVGITGTYQDP
                     GLLGGGANNIQLVAGHRIWLVSPAYAHAVEGVVPDGSLTNGAYAKVKRFVQHVVDIIK
                     SVTQSPHYFGEVAGEYAQAIRDHLPEIIGIVAGFIAAEAASAFFAATPTGVGQIAAVL
                     IQLALSAFGAVGMVEAGVEALKHAGQWLTLAWTAKGKDEQLGAASREFLRMLVSIAMA
                     ALAYTGVKGNLGKAVKIAQAMPPAGMVPALAGAGGRVGPTAVAGEGVKLGVPSPAGPL
                     GTAGGMKMHTDKEGGGSGKSEPKAAADEPAKLDASMSTARPAEQATAGRVAAKAEFKG
                     RTFHAPPPPDPGFDWMDDLGRTFDAMGDGTKSKYFKLDQFKASIGHHLLKGNTFTVID
                     MTGYTADQISAVRAYVDTLPAGKIIRVGF"
     gene            8149..8466
                     /locus_tag="VF516_31645"
     CDS             8149..8466
                     /locus_tag="VF516_31645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112344.1"
                     /translation="MLSICFTEDHEHEWWVSSKVFERLFSSALERGQLDPSLEEWRHV
                     AAANGGLSFSNEERDVARELMTGLRAAASAELAQLGDVDLDTEDGTYKVSLEKLLAAT
                     DVV"
     gene            9245..10267
                     /locus_tag="VF516_31650"
     CDS             9245..10267
                     /locus_tag="VF516_31650"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8112345.1"
                     /translation="MWVVCLAAASSYADGTREGRDAKIAAALRKQCDGGDLMGCSSLG
                     LLYDRGTGVPRDLAKAAALYQKACDGGAMEGCYNLGVTYASSGEGRDVARAVALYQKA
                     CSGGMMLGCFNLGLLHESGDGLARDVGKAVALYRKACDGGAMEGCSNLGAHYLHGTGV
                     DQDAARAAGLFQKACDGGMMVGCSNLGALYAQGTGVVKDAARAVALDQKACDGGDSHG
                     CFNLGLLYQQGAAGARDSAKAAALYQKACDGSLAKGCINLATLYLARGGGVVHDPARG
                     AALARRACDAGDMAGCGLLAALYEQGTGVVRDAAKATALYQKACDGGEPRACSERGVV
                     PRRAPR"
     gene            10539..10829
                     /locus_tag="VF516_31655"
     CDS             10539..10829
                     /locus_tag="VF516_31655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112346.1"
                     /translation="MAALVRLITVLALDSAQALFAERGDVGQGGLRAVPVLGAGNCWA
                     AKAPASDLGDALPDRARGLERTSLVITTVITTSVTRHGGSLAPGYPGDAATT"
     gene            10885..12321
                     /locus_tag="VF516_31660"
     CDS             10885..12321
                     /locus_tag="VF516_31660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112347.1"
                     /translation="MTLSWPPEEGGAKPPPWLRAEATAQLSNGRFRWEFAVEVLGEHR
                     IGVGILIAPVDWGFFGYLGSGHNAFAYDPFLGAIVTETVAIHDDLPVFRATGAGVVAV
                     ELDLVERDSCTFIVDGQPTPPVPVPPGATVIPAVSLLAPGQKVRLRGFEAHRVAASNE
                     RERTERFALASKAVRERIVARSPAGREAARAEAEVQRLAECEEQLRARPDDLALLTRR
                     AELMVRLDIDRALVVEALQQITAMNPTDWLSWRRLAGLLARLDRYEEQLLCWDRLIAG
                     LPASAHSLEADKQGWIPGTEPFASPGRGDAYFLAMRAQCLARLGRQAESLSVWHEAIA
                     RDPNNAWYLADMASDLSAQGSLEAAVRVYDRVMALRPTYWAAAHERCLCLARLGRLDE
                     ALAGLQPLQRRGEFGANALAAYLIYKRNGADAASAALHAIVSANPRFHPLTAELRRTR
                     GLVEAGYAALAESVFEQYAHLRPPQDPA"
     gene            complement(13159..13476)
                     /locus_tag="VF516_31665"
     CDS             complement(13159..13476)
                     /locus_tag="VF516_31665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112348.1"
                     /translation="MYRHVLHRIGLRRFLAVLARVVRLRHQQLAERSHRRVVVPPAQR
                     RRFPGADAREQLQRVDHLTIGRDARVGDDRLDLVARENGFVPPLGILVGERPELALVE
                     HAR"
     gene            complement(13975..>14111)
                     /gene="ssrA"
                     /locus_tag="VF516_31670"
     tmRNA           complement(13975..>14111)
                     /gene="ssrA"
                     /locus_tag="VF516_31670"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         2237 a         4656 c         5062 g         2156 t
ORIGIN      
        1 cgtcgaggcg atgccggtca tccgccagcc gccggcggcg tgctcgtaga gctccaccca
       61 cgcccacgtg gtgtcgagcg gcgtgcgaac gccgtcgtcc tcgcggtacg tgccctcgac
      121 gcggacctgc gcgatcaccc agcccaccgt gccgtcggcc gacacgcgca cgatcggcgc
      181 gacgaggtcc tcgtagcgcg agaactcggc gcgctcgagg tagcgctgga accgcgctcg
      241 ccccatctcc ggtgtgatgc ggctgagctc gccgttgccg atgtcgagca tggcctcgtt
      301 cggcgcgagc gccttccagt cgcgcgcgcg atggtgcgcg agcaccgctt cgtgcgccgc
      361 ccgcagcgca gcctggtcgc tcgaggcgtg cgggtgcgcg gcgccacatg ccgctccgcc
      421 gacgattgcg acgacgatcc aggctctcat gtcatcacga tccacccgca cgcatctcct
      481 gacaatgcga cggcccgcga cacggcgcgc gcgtcgcatc gccgcgcgac gacgatttcg
      541 gtaccactgt cacacgggca agcgatggcg cgaggaagac ctgccggcgc tcggctggcg
      601 ctaccgcgag ccgtacgcga ccaaggcgac gttcatcacg ctggcgatcg aggacggggc
      661 acggccggag gtgatccggg accgggtgac gcacgcgaag atccgccgct ccgcgttcga
      721 cggctacgac cgcgggccgc tctggatcga gacctgcggc gaggtcgcca agctgcagat
      781 cgccggcgcg caccggtcgc ggaggtcgtg gagcttgcta cagcccgtgc tacagctggg
      841 acaatctccg acggagattt gtggtggagg cggcgggagt cgaatccggc tgacgatgcg
      901 ctccacgacg ctccggtcgt ggaatctcgc gcacttcgcc tcatcgaggg taccacgacc
      961 gccgtggagc gcagcggacc gcagcgagct gcagcggaac cgctgcagga tgtagcggat
     1021 cccgtcgctg cggcgatcga ggcggcgctg gcgctgcgcc gctccggggc cgatgcgaag
     1081 gtcctgcgcc gcgcgctgcg ccgtatcgag gagctactgg acgagtagca ggcgctgacg
     1141 ggcggcgctg gtcgccggcg atcgtagtgg ccgacacgac cgacggcccg aggtggggcc
     1201 accgtcgtga tgctgccgcg tcggcggtct ccaccatcag atggagcatt tcggctccga
     1261 aggacatttc gattccgcgg tcgcgactgc aagtgcccgg ctgaactggg atcgcgagcg
     1321 gctcgctgct tgctgtgtat gcccactgga ggcaagcaat gccacgagct cttctcgttc
     1381 tatgtatggt cgtgtgggag atcagcgtgc acatctcggt cacattcgca gatccccagg
     1441 tcgcgtcgaa gcgagtcgcg tacgtcggcg tgcacccgat cccagccgca gaaggcggtg
     1501 gcttctgcaa catcgaaggg ccacacgtcc atatctatcg gccggcgcac gccgaggaat
     1561 tgtaccgcgt catccatggc cgctacctgt tcgtcggtga cccggtaccg ttcggctatg
     1621 agggaccgag gcacgcgtac tccggtcgcc acccggttcc cgtcgatgag ctcgtccccg
     1681 gcgagcaccc ggagcacgac ggcgcggtgt tctgctatct ggacggtccg cactatcaca
     1741 tctacgcgcc cccgccgggg ctcgcgttcg ttctgcgcgg cgacgcgtac tggtacctcg
     1801 gcggctggcc gcgcagctac cagcttgacg ggtcgcgcta ccagaagatc aacgtcatct
     1861 acgcgaagct gacctacgcc cggccggtcg tgaccgccgc ggcgcctgcc ggatatcgcg
     1921 tgccagtcat cgagatcgcg acgccgcggc ctgcgcctgt catcgtgcat gcgccgcccg
     1981 tcgtcaaggt ccgcgcccgg ccagggctga tcgtcgacgg ggacgatgac gacgatgacg
     2041 acgacggtgt ctattactac taccggaggc aaccgcacca tcatcatcat caccaccacc
     2101 accaccacgg ggtggaggtg atcgagatcg tgccatgaac accggtggcg gcccggacgg
     2161 cgcggcgcta ccgcgcatcg cgcagcttgt gacggcggat cagccgctcc agcgacttgc
     2221 gatggacctg cgcgatccgc gccgcctcgt cgaggttccc ctcggtcgcg cgcaggaggt
     2281 cggcgaggta ctcgcgttcg cgctcggccc gcgcagcgcg cagcgtccct tgcttgtgga
     2341 tcgccttctc gaccgagcgc tgcagctcga tcgcggcctg atcgatcacg ccggcgtgga
     2401 tgatcttcgg atcggcgagg atcgcggcgc gctcgaccgc attgcgcagc tcacgcacgt
     2461 tgcccggcca gtcgtggcgg gtcagctcgc cgaacagggc ctcgagcatc tgcggcggca
     2521 gcggcggacg tgcgcggttg ccgagcgcgg ccgccgcgaa gtgcgccgca agcaagggaa
     2581 tgtcgtcgcg gcgatcgcgc agcgcgggaa cggtcacgcg gatcacggcg agtcgatagt
     2641 agagatcggc gcggaaccgg ttctccgtga ccatgcgcgg caggtcacgg ttggtggcgg
     2701 cgatgacacg gatgtcgacg cggatcgtcc ggtcgccgcc cacgcgagtg aactcgccgc
     2761 tctcgaggca gcgcagcagc ttgggctgga gctcgatcgg tagctcgccg agctcgtcga
     2821 ggaacaccgt gccgctgtcc gcgcgctcga acacgccggg ccgcgtggcg acggcgccgg
     2881 tgaacgcgcc gcgctcgtgc ccgaacagct cactctcgac cagcgacggc gggatcgccg
     2941 agcaatcgag cacgaccagc ggcttgccgg cacgcaggga gcgccggtgg atcgcgcgcg
     3001 cgaccagctc ctttcccgtc ccggtctcgc cctcgatgat caccggcgcg tcggtcggcg
     3061 cgacccgcgc gcacaccgcg aacagttcgc gcatcgcgac cgagcggccg atcaggccct
     3121 cgaactggtc gtcctcggcg agccggacct cgaacggctc gtcaagcagg cggaatcgga
     3181 ggcgcgtcgc cccgagctcg atgagcgcac cgtcctccag gcatgcgtcg cgcacgcgcg
     3241 tgccgccgac gcgcgtgccg ttggtcgagc cgagatcgcg cagcacgtac tcggcgtcct
     3301 cgacatcgag ccgcgcgtgc agccgcgacg cgtgccggtc ggcgagcacg acatccgccg
     3361 aggcgtgcgt gccgaacaag aggctgcgct tgtcgccgcg gatcgagcgg ccggcgtccg
     3421 ggccggacag gaactcgagc gcgaagcccc gaacctggat cgccgtggct cggcctccga
     3481 tctgcagcag ggtagtcgcc ggtcggtgct ccgagcccat cgccatgatt gtatcgggtg
     3541 tgtcgggccc gttccgccgc acgctcgaga taagatggcg cggcgtgccg agcgaagtcg
     3601 aacgtacgat gccgcttgcg atcgccgaca tcgctggccc cctcctcgcc ggacgctatc
     3661 gtctgcgcgc gctgctcggt cgcggcggca ccggcgaggt ctacgaggcc gaggacatcc
     3721 ggacgggcgc ggcgctggcg atcaagaagc tggccccagg cgccggcgac gcgacgaggt
     3781 gcgccgagcg cttccggcgc gaggccgagg cggcgggcct gctccgccac ccgaacatcg
     3841 tcgaggtgct cgatttcgtc tcggagcaag actcgctgta cctggtgatg gagctcgtgc
     3901 gcggccgctc ggtcggcgcg ctcatcgaca gcggcgagct gacggcgcgc cgcacgcttg
     3961 tgatcgcccg gcaggtgcta gaggcgctcg cgcacgcgca cgcgcgcggc atgatccacc
     4021 gcgacatcaa gcccgacaac ctcatgctcg tctccgccgg tgagccccga cgcgagtacg
     4081 agcgcgtcaa gctgctcgac ttcgggctgg tgaagtggat cggcgacccg gccggcgggc
     4141 tcggcggcga gcagctcacg cagaccggcg ccgtgttcgg tacccccgcg tacatggcac
     4201 ccgagcaggc gctcaaccga ccgctcgacg agcgcgtcga tctgtacgcg ctcggggtcg
     4261 tcgtgttcga gatgctcacc ggccggccac cgtttcgcag tccggatccg atgacgctcc
     4321 tgcggatgca ggcgtcggcg ccgccgccgg cgctgtcggc gatcgctctc ggccagccgt
     4381 ggtgcacgcc cgcgctcgag cagctcgtga tgcgcgccct ggccaaacag cccgccgcgc
     4441 ggttcgcgag cgcgctcgag atgatggccg ctctcgaggt cgcgttcctc tcgctcgatc
     4501 acctgccagc cggcggctga acggcggtcc accgtgcctc ggtcccggct ggactgccgc
     4561 gaggtggctg ccgaacacgt cggcgaccac ccacacgatt cgcccgcgat cttgccttca
     4621 tccacgggga tggacgcacc ggtcatgtac gaggcgcttg gcgatgccac aaaggcgatc
     4681 acatgagcga tcttctctgg tgtcgccgga cgtttgacgg gagcactcga gatgagcgcg
     4741 accttttcgt ctgtgctgtg aaccgcgcaa gcattcccgt attcgttgtg cccgggagcg
     4801 actgtgtttg cgcgaactct ggttcctgca actttcagtg cggcggattt ggtcattcct
     4861 tccaccgcat cgtcggcacc gccgagactg atatgcgtgc tcggagatcg tgcgcctggt
     4921 ggtggcggac cgcgcgacgt caagagcgtg gagattccat tgaccttcgt gcgcgcgacg
     4981 tggcgaacgt ccgcaggctg gaatgtgtcg atcgacggaa tggcctttga gtacgtcgtc
     5041 aacaaacgag agggggtatt tcccgcctgg cggatgcacg atctggctat tgaagtgaga
     5101 cccgtaccag gttagttggc ttttgttgtg acccttggcg tcgaccaggc gatcgggcgg
     5161 ggtaggagcg aggagggaca tgcggcggca gggtatgtgg tggtggaggc ggcgggagtc
     5221 gaatccggct gacgatgcgc tccacgacgc tccggtcgtg gaatctcgcg cacttcgtct
     5281 ggtcgacggt accacgaacg ccgtggagcg cagcggaccg cagcgagacg cagcggaacc
     5341 gctgcaggac gtcgcggatc ccgtcgctgt ggcgctcgag agcgcgctgg cgctgcgccg
     5401 ctccggcgcc gatgcgaagg ccctgcgccg cgtgctgcgc cggatcgagg agctcctgga
     5461 cgagtagcca ccagctcggc ccgacgcact ggacgtcggg cacgtgctcg acgccgcggt
     5521 ggtggcgctg gcggcggcga tctcgaagca gctcggcacc ggcgtgctgc cgcggggcgg
     5581 cggcgctgtc gaccacgagg agcgagcact ggtcgcggct cgggtgctcg gggggctcgc
     5641 cggcgacgtc gcccaggagt tgcctcgggt cggcggcggc ggcggtggcg tcacggtccc
     5701 ggcccggcgc tcgcggtggc gctaccacca ggttccaggc gctccagcaa ctggttgcgc
     5761 aaggctggtc gatatgctgg atctcgtcgg tggcttgagt ttcccaggaa acccggtagc
     5821 ctctctggcg tcgtgcacgg caagagcggg cgaggaacga gcacaacgca agatcggcag
     5881 ggcgatcgct cgggatcggc gcacgttggt aagcgaaccc tcgtcgagca aatcgctgcg
     5941 ccgcgagcga tgtcgtctcc ggtagggcca ggcgcggccg tcaactcggc gagcgggttg
     6001 cctcgcctgc agttgaaggg catcccaggc cacgcctccg tcggaatcga tggcgcggcc
     6061 gtgcaacgca aagcacaggg agaagcagcg cccgacgggg catcgccgac cgagactgcg
     6121 ggatccgtag gcgcggcggg tgacgacggc gcggttcatc gcgtcgcggc ggctggggtc
     6181 tccggcacgg gcgagcggct gccgcacctc gagcgcatcc aaaacgcgtt cgggccggac
     6241 cacgatatgt cgggtatcaa ggcgcacgtc ggtagtgcgg cgggcgaagc agccgcgcag
     6301 atcggcgccc gcgcctacgc gacggggaac aacgttgcat tcgccgggga acccgacgta
     6361 cacaccgccg cgcacgaagc ggcgcatgtc gtgcagcagc ggggcggcgt acagttgaag
     6421 ggcggcgtcg gcgcggcggg cgacgcgcac gaggtgcacg ccgacgcggt cgcggatcgc
     6481 gtcgtccgcg gcgagtccgc ggcagagttg ctggcgccgt atcgcggcgg tgagacgatg
     6541 actggcggcg tccagcgcgc gcctgcctcg ccgggttccg ctgccgacat tgcagcgccg
     6601 accggcggca tcaacctgcc tgggttcatc gacacgagcg acggcgcgaa cctgcgcacc
     6661 gggccggcgg aggcgggcgg caagaccgtg atcgacgcgc cgctgccacc cgcaacacgc
     6721 gtcttcgtga gcggtacata tccgagcgcg cccgagtggt ggtacgtgac cgcgttcctg
     6781 ccggacaaga tcgtgcgcgg gtatgtccag cagttccgcg tcaacacgga tctccccgag
     6841 ccgctcgcga agctgcatca ggtgaaatcg ggcgacacgg tcgagaagct cgcagtccag
     6901 gagtacgcat cgtcggttcg cgacggccac gacctgcgct actacgagaa cgtgctcctg
     6961 ttcgccaaca agcagcgcgg gcgcgtcggg atcacaggca cgtatcaaga tcccgggctg
     7021 ctgggtggtg gcgccaataa catccagctc gtggccggac accgcatctg gctcgtgagc
     7081 ccagcgtacg cgcatgcggt cgagggcgtc gttcccgatg gatctctgac gaacggcgcg
     7141 tacgcaaagg tcaagcggtt cgttcagcac gtcgtcgaca tcatcaagag tgtcacgcag
     7201 tcgccgcact atttcggcga ggtcgcgggc gagtatgcgc aggcgatccg ggatcacctg
     7261 ccggagatca tcggcatcgt cgcggggttc atcgcggccg aggcagcatc agcgttcttc
     7321 gcggccacgc caacaggcgt gggccagatc gctgccgtgc tgatccaact cgcgctctcg
     7381 gcgttcggcg cggtcgggat ggtggaggcg ggcgtcgagg cgttgaagca cgcgggtcag
     7441 tggttgacgc tcgcgtggac ggcgaagggg aaggacgagc aactcggcgc cgcgagccgc
     7501 gaattcctgc gcatgctggt gagcatcgcg atggcggcgc ttgcgtacac cggcgtgaag
     7561 ggcaatctgg gcaaggccgt caagatcgcg caggcgatgc cgccggccgg catggtgccg
     7621 gcgctcgcgg gcgcaggcgg cagggttgga ccgacagccg tcgctggcga gggagtcaag
     7681 ctcggcgtgc cgagcccagc gggaccgctc ggcacggccg gcgggatgaa gatgcacacc
     7741 gacaaagagg gcggcggctc gggcaagtcg gagccaaagg ccgcggcaga cgagccggcg
     7801 aagctcgacg cttcaatgtc gaccgcccga cccgccgagc aggcgaccgc gggacgtgtc
     7861 gcggccaagg cggagttcaa gggccgtacc ttccatgcgc caccgccccc ggatcccgga
     7921 ttcgattgga tggacgatct gggccgcacg ttcgacgcca tgggcgacgg caccaaatcg
     7981 aagtacttca agctcgatca gttcaaggcc tcgatcggtc accacctgct gaaaggcaac
     8041 acgttcaccg tgatcgacat gaccggctat acggctgacc agatctctgc ggtacgcgcg
     8101 tacgtcgata cgctccccgc aggcaagatc atccgagtgg ggttctgaat gctatcaatt
     8161 tgcttcaccg aggatcacga gcacgagtgg tgggtttcga gcaaggtctt cgaacgcctg
     8221 ttctcgagtg cgctcgaacg tggtcagctc gacccgtcac tggaggagtg gcggcatgtg
     8281 gccgccgcca acggcggcct ctcctttagc aacgaggagc gagatgttgc gcgtgagctg
     8341 atgactggtc tgcgcgcagc ggccagcgca gagctggcgc agctcgggga tgtggatctg
     8401 gacaccgagg acgggaccta caaagtgtca ttggaaaagc tgctagccgc caccgatgtt
     8461 gtttgagtat cgctcatggt gaagcatggc agctgttcga gaacagctgg ggctgtcggc
     8521 tacgaggagc gagcgctggt cgcggtgggc ggcggcgacc tcgagagatc gacgaggcgc
     8581 tgcagcggga cggcgagcgc ggtggcggcg gcgcaccggc ccggcccggc gctcgcgctc
     8641 gaccgcagtg ctcgtgctgg acgtcgccca ggcgttgctc gccgaacgct gcgggcggtg
     8701 gcaatggtcg gcaccccaca accatgccgg gctggaaatc gccgacgacc tcggcctggg
     8761 ccgggggcct ggcggtgaag cagcgcgcca atcacggcga cggacatggc gtcactggct
     8821 cggccgtagc tctcggcgcg cgtgctggac atcgcccagg agctgcagga gctcgtatgg
     8881 ctcgcggtgg gaggcggcgg ctgttcgaga gagtctagaa gcgcagcact ctcttgtgtg
     8941 cgagaagcga gaggctgata ctgaaatcac cctgaaatac aatcccggtc cctgcgggaa
     9001 tcatattcat tgcgtccatc gatcccgtgt atgtattccg cggcaactcg attgctagac
     9061 accaattccg gaacaggatg cgctcgccag cgccgactaa gtttgcgaga agttgctagg
     9121 atgcttcccg gtctctgatc ggagccggga agacgtgcag gtccacggct ggaggctcga
     9181 ccagctgccg cgcgcgttcg ctcatcacac tttggaggtc aagatgcgat ggtcaggagg
     9241 tgccgtgtgg gtcgtctgtc ttgcggccgc gtcgtcgtac gcggacggga cccgcgaggg
     9301 ccgcgatgcc aagatcgccg ccgcgcttcg caagcaatgc gacggcggcg atctgatggg
     9361 ctgctcgagc ctcggcctgc tctacgaccg cggcaccggg gttccgcgcg atctcgccaa
     9421 ggcagccgcg ctctaccaga aggcatgcga cggcggtgca atggaaggct gctacaatct
     9481 cggggtcacg tacgcgtcca gtggcgaggg ccgggatgtg gccagggccg tcgcgctgta
     9541 ccagaaggcg tgcagcggcg gaatgatgct cggctgcttc aacctcggtc tgctccacga
     9601 gagcggtgac ggcctcgcgc gcgatgttgg caaggcagtg gcgctctacc gaaaggcgtg
     9661 cgacggcggc gcaatggagg gctgcagcaa cctcggcgcc cactacctgc acggcaccgg
     9721 ggtggatcag gacgcagcca gggctgccgg gctgtttcag aaggcatgcg acggcggtat
     9781 gatggtgggt tgttccaacc tcggcgccct ctatgcacag ggcaccgggg tcgtcaagga
     9841 cgcagccagg gcggtggcgc tcgatcagaa ggcatgcgac ggcggcgaca gccatggatg
     9901 cttcaatctc ggtctgctct accagcaggg cgctgccggt gctcgcgact ccgccaaggc
     9961 agcggcgctc taccagaagg catgcgacgg cagcttggca aagggctgca tcaacctcgc
    10021 caccctgtac ctggcgcgcg gtggcggcgt cgtccacgac ccggccaggg gcgccgcgct
    10081 cgctcggcgc gcgtgcgacg ctggcgacat ggccggctgt ggtctcctcg ccgccctgta
    10141 cgagcagggc accggcgttg tccgcgacgc agccaaggct actgcgctgt accagaaagc
    10201 gtgcgacggc ggcgagccgc gggcgtgctc cgagcgcggc gtcgttccgc gtcgggcacc
    10261 gaggtagtcg tacctcgctc gccgtctcat ggtgtcgctg ctgcgcgagg gtgtaatccg
    10321 ggcgtggcgt cctgggacgg cgcacgtgct cgaggcgatc tggcactgtt cggccttggt
    10381 gatgtggccc cgtgcgtggc tgcgtccgcg cgctccgaga gctcggcgag cgctgcagtg
    10441 ggtggcggcg ctggcgttgc cggcctgctc ggcgccacgc tgctcggtgc tctctggcga
    10501 cgtcgcctag ggcgctgcag gagctcgggc tggtcgcggt ggcggcgctg gtgcgattga
    10561 tcacggtgct tgcgctggac agcgcacagg ctctgttcgc cgagcgcggc gacgtgggcc
    10621 agggcggcct gcgcgcagtc cccgtgctgg gcgctggcaa ctgctgggct gccaaggcgc
    10681 cggccagcga cctcggcgac gccctccctg accgcgctcg ggggcttgag cgcacgagcc
    10741 tggtgatcac caccgtgatc accacctcgg tgacccgaca cggaggtagt cttgcgccgg
    10801 gatacccagg cgatgcagcg accacctgac ctcgaatgga acaggtccgc ggttcagaca
    10861 cctctgcgga tcgccgatga cggggtgacg ctgtcgtggc cacccgagga gggcggcgcg
    10921 aaaccgcccc cgtggctgcg ggccgaggcc actgcgcagc tctcgaatgg acggttccgg
    10981 tgggagttcg ccgtcgaggt gctcggcgag caccggatcg gggtggggat cctgatcgcg
    11041 cccgtcgact ggggcttctt cggctacctc ggctcggggc acaacgcgtt cgcgtacgat
    11101 ccgttcctgg gagcgatcgt caccgagacg gtcgccattc acgacgacct gccggtgttc
    11161 cgtgccaccg gcgccggcgt ggtcgcggtg gagctcgacc tcgtcgagcg cgacagctgc
    11221 acgttcatcg tcgacgggca gccgacgcca cctgtgccgg tgccgcccgg cgcgacggtg
    11281 atccccgccg tgagcctgct cgcaccgggc cagaaagtac ggcttcgcgg attcgaagcg
    11341 catcgcgtcg cagcaagcaa cgagcgcgag cgaaccgagc gattcgcgct cgccagcaag
    11401 gccgtccggg aacgcatcgt ggcacgcagc ccggccgggc gggaagcagc ccgcgcagag
    11461 gcggaggtcc agcggctcgc cgagtgcgag gagcagctgc gcgccaggcc ggatgatctc
    11521 gcgctgctca cccggcgcgc cgagttgatg gtgcgactgg acatcgaccg cgcgctcgtc
    11581 gtcgaggcgc tccagcagat cacggcgatg aatccgaccg attggctatc gtggcgccgc
    11641 ctggcagggc tcctggctcg gctcgatcgc tacgaggagc agctcctgtg ctgggaccgg
    11701 ttgatcgccg gtctgcccgc gtcggctcac tcgctcgaag ccgacaagca gggctggatt
    11761 cccgggaccg agcccttcgc gtcgcccggg cgcggcgatg cctacttcct ggccatgcga
    11821 gcgcagtgcc tggcacggct cgggaggcag gccgagtcgc tctcggtatg gcacgaggcg
    11881 atcgcgcgcg acccgaacaa cgcatggtac ctcgccgaca tggcgagcga tctctctgcg
    11941 caggggagcc tggaagcagc ggtgcgggtc tacgaccggg tcatggcgct ccgtccgacg
    12001 tactgggccg ccgcgcacga gcgctgcctc tgtctcgcaa gactcggtcg gctagacgag
    12061 gcgctcgccg ggctgcagcc actccagcgc cgcggcgagt tcggggccaa tgcgctcgcc
    12121 gcgtatctca tctacaagcg gaacggcgcc gatgcggcca gcgccgcgct gcatgcgatc
    12181 gtgagcgcga atccccggtt tcatccactg acggccgagc ttcgccgcac ccgcggcctg
    12241 gtggaagccg gatacgcggc gctcgccgag tcggtcttcg agcaatatgc ccacctgcgg
    12301 cccccgcagg atcccgcgta ggcgaggccg ctcggatcca acgcagagcg cgggtcgggc
    12361 tgtaacggct ggaactcgct gttacagccc ggcgtcgatt ccgtcatcgc agctcctcgc
    12421 tccgacgatc gcgcttgatg cgtacaccgc gtcgaactac aatgcagtgc gacgtgatga
    12481 cgatcgatga cgaaacggcg gtgtgacgat ggagaagaca gcacagacca cgatccgcat
    12541 gaccgcggcc gagcgcgagg catgggaagc ggctgcgaag gccgatgggc gcacgctgtc
    12601 ggcatggatc gtgcggcggt gtaacggtca gccgacgatc gagccagtgt tcgcggcgcc
    12661 gcgagcggca cggaagcgac agcccaagcg aaaggcgcgg tgatgggggt ctacgcgcgc
    12721 gactcgaagc tgtggatccg gttccgcgat gcggacggca ggtggcgcga cgcctcgacg
    12781 gggttcagcg tggggcagga ggagctcgct caggtcatgc acgatgagac cgtggcgcgc
    12841 atcgcggcgg cgacgcggac ggtgaagcac gcaccggtcc ggggcctcac cgtgcgtgcg
    12901 ttcgtggccg agtggcttcc caagcgacgc gagcggggcc tcgactggaa gtccgacgag
    12961 ggccgactca ggttgcacgt gctgccctac atcggagatc tgccgctcgc cgaggtccgc
    13021 gcgcgccacc tggtggacct gttccacaag ctgcggaccg accgcgagcg gaacctggcg
    13081 cagcggacca tctacaacat ctattcggtc atctcggcga tgttccgcga tgcgaagctc
    13141 gccgacctga tcgaccagtc accgtgcgtg ctcgacgagc gccagctcgg gccgctcacc
    13201 gacaaggatc ccgagtggcg gcacgaagcc gttttcacgc gcgacgaggt cgagacgatc
    13261 atctccgacg cgcgcatccc ggccgatcgt caggtggtct acgcgctgga gctgctcgcg
    13321 ggcgtccgca ccggggaagc ggcggcgttg cgctggcggc actacgacgc ggcgatgcga
    13381 ccgctcggca agctgctggt ggcgaagtcg tacaacacgc gcaagaaccg cgagaaatcg
    13441 acgaagaccg atgcggtgaa gcacgtgccg gtacacccgg tgctcgcggc gatgctcgcc
    13501 gagtggaagc ttggcggctg ggccgagatg atggggcgtg cgccgacggc ggatgatctg
    13561 atcgtgccgc taccgcccga cgcggccgag cgtcgccgat cgcgcaccgg cgagccgttc
    13621 cgcggccacg actacagcgg caagcggtgg cgcgacgagg acctcccggc gctcggctgg
    13681 cggcaccggc ggcactacga catgcgggcg acgttcatca cgctggcgat cgaggatggc
    13741 gccgatccgg agatcctgga gacgcgcgtg acgcataccc ggaagtcgcg caacgcgttc
    13801 gacggctaca accgcgggct gcactgggag cggacgtgcg ccgagatcgc gaagcttcgg
    13861 atcgcgcgcg ggccgcgaag ctcggcgatc gagcagccga tcgcggccgg agcgggcccc
    13921 gattcgctgc aatccgctgc agcactcgca actaagcaga ataacgatgg aaaatcgtgg
    13981 aggcggcggg agtcgaaccc gcgtccgaag atggatcagg acgagcttct acgcgtgtag
    14041 gcacgccgtt tgaggtctcg cgctcgcagg cggcggccga cctgcgagcg caacgagcca
    14101 gcgagatctc g
//
LOCUS       DASVOR010001696         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_222033,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001696 DASVOR010000000
VERSION     DASVOR010001696.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222033"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF516_31675"
     CDS             complement(<1..256)
                     /locus_tag="VF516_31675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112349.1"
                     /translation="MSGAPEAEDVRSAVPIPDLTPIDTALQAGGAELRAAVKRFRPAD
                     LGRDLSRRPIAEGRAILDAIDDRRGAAMLRAAHPVVAAQLL"
     gene            381..>1521
                     /locus_tag="VF516_31680"
     CDS             381..>1521
                     /locus_tag="VF516_31680"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8112350.1"
                     /translation="MTDARFPLETACRLSRSVLWRLQRRFFERRGIAAWSEGVVPHHV
                     TCNPYLARAYARVILGFVRDWRDRLDPAQPLYIVELGAGSGRLAFHVLRQLEPMLAEP
                     QWRGVRVRYVLTDFAEANLAAWRAHPQLAPWLDAGRLDLARFDAEHDGSITPERTGET
                     LAPGRVANPVIAIANYVFDGLPLDGFSVTGGRLHEWRVRLCATHPGELDDPELLERVR
                     LTCELCPVEPAGYYGDPALDRVLDEHRARLPDTAFTFPSASLGCVARLAELAGGRLLV
                     LSTDKGEVAEDGLRGQAGPQLTLHGSFSIAVNYHAIARWVGQQGGEALVPAGRPRTIA
                     TCGFVLGPPPGGAAETRRAYAEAVELQQPDDLFAIEQGLEPAHASL"
BASE COUNT          177 a          583 c          557 g          204 t
ORIGIN      
        1 cgagcagctg ggcggcgacc accgggtgcg ccgcgcgcag catcgccgca ccgcgccggt
       61 cgtcgatcgc atcgaggatc gcgcggccct cggcgatcgg ccggcgcgac aggtcgcggc
      121 cgaggtcggc ggggcggaac cgcttgaccg cggcccggag ctcggcgccg ccggcctgca
      181 gcgcggtgtc gatcggcgtc agatcgggga tcggaacggc gctgcggacg tcctcggcct
      241 cgggagctcc actcatgatc ccaccttccg cgatcgagcc ccgaggggca atccgcccgg
      301 gatccaaatg tcgttaagac ggcagggtgt ggaacgccgt gtgacgaccc ggtcgcgccg
      361 ggcggtgata ggttgccgcc gtgaccgacg cgcgcttccc gctggagacc gcgtgccggc
      421 tgtcgcgctc ggtgctgtgg cgcctgcagc gccggttctt cgagcgccgc ggcatcgccg
      481 cgtggagcga gggcgtcgtg ccgcaccacg tgacctgcaa cccgtacctc gcccgggcct
      541 acgcgcgggt catcctgggg ttcgtgcgcg actggcgcga tcggctcgat ccggcgcagc
      601 cgctgtacat cgtcgagctc ggcgccgggt ccggccggct ggcgttccac gtcctgcgcc
      661 agctcgagcc gatgctcgcc gagccgcagt ggcgcggcgt ccgggtgcgc tacgtgctga
      721 ccgacttcgc cgaggccaac ctcgccgcct ggcgggcaca tccccagctc gcgccgtggc
      781 tcgacgccgg ccggctcgac ctcgcacggt tcgacgccga gcacgacggc tcgatcacgc
      841 cggagcgcac cggcgagacg ctggcgcccg gtcgcgtcgc caacccggtc atcgcgatcg
      901 ccaactacgt gttcgacggc ctgccgctcg acgggttctc ggtcaccggc ggccggctcc
      961 acgagtggcg ggtccggctg tgcgcgaccc accccggcga gctcgacgat cccgagctgc
     1021 tcgagcgcgt ccggctgacc tgcgagctct gcccggtcga gcccgccggc tactacggcg
     1081 acccggcgct cgaccgcgtg ctcgacgagc accgcgcgcg cctgcccgac accgcgttca
     1141 cgttcccgtc ggcgtcgctg ggctgcgtgg cccggctggc cgagctcgcc ggcggccgcc
     1201 tgctcgtgct gtcgaccgac aagggcgagg tcgccgagga cgggctgcgc ggacaggccg
     1261 ggccgcagct caccttgcac ggcagcttct cgatcgcggt caactaccac gcgatcgcgc
     1321 gctgggtggg ccagcagggc ggcgaggcgc tggtccccgc cgggcgcccg cgtacgatcg
     1381 cgacctgcgg cttcgtgctc gggccgccgc cgggcggtgc cgccgagacc cgccgcgcct
     1441 acgccgaggc ggtcgagctc cagcaacccg acgacctgtt cgcgatcgag cagggcctcg
     1501 agccggccca cgcgtcgctc a
//
LOCUS       DASVOR010001697         4291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_251517,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001697 DASVOR010000000
VERSION     DASVOR010001697.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251517"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VF516_31685"
     CDS             <1..763
                     /locus_tag="VF516_31685"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8112351.1"
                     /translation="PETGAPYGSRYPWVTVEDMIEAQRRIADHLGIARWKSVIGGSLG
                     GMQVLMWAALYPERVGSLVCVAAAAAVPVASVGWHLIGRKLIQSDPAFRGGDYYGTGE
                     VQRGLQLARMVGHMTYLSEAALEQKFGRRRRGGTRQFEVDSYLEYQAAKFANAYDANS
                     YLRVQAAMDEMDLAEKFGSLTAAFRALRAPALLVSFDTDWLFPPDETRRIHAAMRAAD
                     VSATHLEIATPNGHDAFLVDYPLITPPVSDFLASL"
     gene            complement(824..1417)
                     /locus_tag="VF516_31690"
     CDS             complement(824..1417)
                     /locus_tag="VF516_31690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEX8112352.1"
                     /translation="MTTRHDAARASFTHMEASTQDDWQMIGAEFARFAGGLADRVLKH
                     LALLDGDYGGFPVDRLTHSLQTATRAHADGRDEEYVVCALLHDIGDTLGTFNHPDIAA
                     AILRPFVSEENLWMVEKHGIFQGYYFFHHVGMDRNMREAFRSHPYFARTEEFCARYDA
                     PAFDARGETRPLAFFEPMVRRVLAQPRQSIYTAVNSR"
     gene            1499..2317
                     /locus_tag="VF516_31695"
     CDS             1499..2317
                     /locus_tag="VF516_31695"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8112353.1"
                     /translation="MSQEPSAELASHLERMKRDGYTIVENVIDAEAVRALRDDVRRLE
                     RELATRPAANLFEGVRTLRIYNLLVHGALYHQIPIHPRVLPIIEGVLDRGCLVSSLSS
                     IDIGPGETAQPLHADDQLIPLPKPHVSIICNTMWALTDYTEANGATRLVPGSHRAERS
                     PQPFGEPVETVAAEMPAGSVLVFDGSIWHGGGANHTDEFRLGIAMNYCAGWLRQQENQ
                     QLGIPAEIAREFPPRLRKLVGYGLYKGLIGHIDKSTPEYLLDGGPPTPVVGFMK"
     gene            2330..2644
                     /locus_tag="VF516_31700"
     CDS             2330..2644
                     /locus_tag="VF516_31700"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEX8112354.1"
                     /translation="MTKTAHKTIGDIAPKLAQLTDDVLFGDVWERPQLSKRDRSLITC
                     AALIATGKTEQMSFHFPRAIENGVTQDELVEMITHLAFYVGWPGAMSAITRAKELFTK
                     QG"
     gene            complement(2646..3743)
                     /gene="dnaJ"
                     /locus_tag="VF516_31705"
     CDS             complement(2646..3743)
                     /gene="dnaJ"
                     /locus_tag="VF516_31705"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEX8112355.1"
                     /translation="MRDLYEVLEVDRQVSPSELKKAYYRLAKKYHPDHNPGDKDAEEK
                     FKEASNAYSILSDDEQRQRYDRFGFDGIRSVNGNAGGYTNVEDILSAFGDIFGDFFGG
                     RGAGRRQPRGADLRVDLNLTFAEAVWGITKDVKVSREIACATCGGNGARPGSKPEICR
                     TCQGKGQVVHAQGFFMVQTTCPHCRGAGKSIKDPCDDCRGRGLRPETSTLSVTVPAGV
                     DDGQTLRLAGKGETAPGGTTGHLYVVLHVQGDERFKRDGEDVLTEVPISFVKAVLGGE
                     VEVHTLDEHCTASATVELKPGIQPDEVIVRRGQGIPRVGESGRGDHVLQFKVDIPKKL
                     TPRQEELMRELATELGETVRAEKRGLFHRKK"
     gene            3807..>4291
                     /locus_tag="VF516_31710"
     CDS             3807..>4291
                     /locus_tag="VF516_31710"
                     /inference="COORDINATES: protein motif:HMM:NF015655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112356.1"
                     /translation="MIRPADLARGAPDAADALRRAERLFDAAAQSALDLAGLGDPAQR
                     IATLCCQSAPYLATLLTRDPHRLGRVASDPYLRREKPREVLVAEVTARAAPARTPAEL
                     QAALRSVRADELVRLGVRELELGLDTEVGRELARLADACFDAAIGFWDRALRARYGAP
                     R"
BASE COUNT          619 a         1504 c         1512 g          656 t
ORIGIN      
        1 ccccgagacc ggcgcgccgt acggctcacg ctacccgtgg gtcaccgtcg aggacatgat
       61 cgaggcgcag cgccggatcg ccgatcacct cgggatcgcg cgctggaagt cggtgatcgg
      121 cggctcgctc ggtgggatgc aggtgctgat gtgggccgcg ctgtaccccg agcgcgtcgg
      181 ctcgctggtg tgcgtggctg cggcggccgc cgtgccggtc gccagcgtcg gctggcacct
      241 gatcggccgc aagctgatcc agagcgatcc ggcgttccgc ggcggcgact actacggcac
      301 cggcgaggtc cagcgcgggc tgcagctcgc ccgcatggtc ggccacatga cgtacctgtc
      361 cgaggccgcg ctggagcaga agttcgggcg gcgccggcgc ggcggcaccc ggcagttcga
      421 ggtcgactcc tacctcgagt accaggccgc gaagttcgcc aacgcgtacg acgcgaactc
      481 gtacctccgg gtgcaggcgg cgatggacga gatggacctc gcggagaagt tcggctcgct
      541 gaccgccgcg ttccgtgcgc tgcgcgcccc ggcgctcctg gtatcgttcg acaccgactg
      601 gctattcccc cccgacgaga cccggcggat ccacgccgcg atgcgcgcgg ccgacgtctc
      661 cgcgacccac ctggagatcg cgacgccgaa cgggcacgac gcgttcctgg tcgactaccc
      721 gctgatcacg ccgccggtct cggacttcct cgcgtcgctg tgagccgccg gtcctcggcc
      781 ggtcagcggg acacggctcg cggccgaccc gccgatggcg aggtcagcgg ctgttgaccg
      841 cggtgtagat cgactgccgg ggctgggcca ggacccggcg gaccatcggc tcgaagaacg
      901 ccagcggccg ggtctcgccc cgggcgtcga acgccggcgc gtcgtacctg gcgcagaact
      961 cctcggtgcg cgcgaagtac gggtggctgc ggaatgcctc gcgcatgttg cggtccatgc
     1021 cgacgtgatg gaagaagtag tagccctgga agatgccgtg cttctcgacc atccagaggt
     1081 tctcctcgga cacgaacggc cggaggatcg ccgcggcgat gtcgggatgg ttgaacgtgc
     1141 cgagcgtgtc gccgatgtcg tggagcagcg cgcacaccac gtactcctcg tcgcgcccgt
     1201 cggcgtgggc ccgggtcgcg gtctgcagcg agtgggtcag ccggtcgacc gggaatccgc
     1261 cgtagtcgcc gtcgagcagc gcgaggtgct tgagcacgcg gtcggccagc ccgccagcga
     1321 accgggcgaa ctcggcgccg atcatctgcc agtcgtcctg ggtgctggcc tccatgtggg
     1381 tgaagctcgc gcgcgccgca tcgtgccggg tcgtcatctc gggtgcgacc ttaccaccga
     1441 ccggccgcgc cgcaggcaac ctcttgaccc ggagccgccg accgccgaaa acggcggcat
     1501 gtcgcaggag ccgagcgccg agctggcgag ccacctggag cggatgaagc gcgatggcta
     1561 caccatcgtc gagaatgtga tcgatgccga ggccgtccgc gcgctgcgcg acgacgtccg
     1621 ccgcctcgag cgcgagctcg ccacccggcc ggccgcgaac ctgttcgagg gcgtgcgcac
     1681 gctgcggatc tacaacctgc tggtgcacgg cgcgctctac caccagatcc cgatccaccc
     1741 gcgcgtgctg ccgatcatcg agggcgtgct cgaccgcggc tgcctggtgt cgtcgctgtc
     1801 gtcgatcgac atcggcccgg gcgagaccgc ccagccgctg cacgccgacg accagctgat
     1861 cccgctgccc aagccgcacg tctcgatcat ctgcaacacg atgtgggcgc tgaccgacta
     1921 caccgaggcc aacggcgcga cccggctggt ccccggctcg caccgcgccg agcgctcgcc
     1981 gcagccgttc ggcgaaccgg tcgagaccgt cgccgccgag atgccggccg gcagcgtgct
     2041 ggtgttcgac ggctcgatct ggcacggggg cggcgccaac cacaccgacg agttccggct
     2101 cggcatcgcg atgaactact gcgccggctg gctgcgccag caggagaacc agcagctcgg
     2161 catcccggcc gagatcgcgc gcgagttccc gccgcggctg cgcaagctgg tcggctacgg
     2221 cctgtacaag ggcctgatcg gccacatcga caagtcgacg cccgagtacc tgctcgacgg
     2281 cggcccgccc acgccggtcg tcggcttcat gaagtgagga gaacgtccga tgaccaagac
     2341 cgcccacaag accatcggtg atatcgcgcc caagctcgcc cagctgaccg acgacgtgct
     2401 gttcggagac gtctgggagc ggccgcagct gtcgaagcgc gaccggagcc tcatcacctg
     2461 cgcggcgctg atcgcgaccg gcaagaccga gcagatgagc tttcacttcc cgcgcgcgat
     2521 cgagaacggc gtcacccagg acgagctcgt cgagatgatc acgcacctgg cgttctacgt
     2581 cggctggccc ggcgcgatgt cggcgatcac ccgggccaaa gagctgttca ccaagcaggg
     2641 ctgaactact tcttgcgatg gaacagaccg cgcttctcgg cgcgcacggt ctcgccgagc
     2701 tcggtggcga gctcgcgcat cagctcttcc tgccgcggcg tgagcttctt ggggatgtcg
     2761 accttgaact gcaggacgtg atcgcctcgc ccgctctcgc ccacccgcgg gataccctgg
     2821 ccgcggcgca cgatgacctc gtcgggctgg atcccgggct tgagctcgac ggtggccgag
     2881 gcggtgcagt gctcgtcgag cgtgtggacc tcgacctcac cgccgagtac cgccttgacg
     2941 aagctgatcg gcacctcggt cagcacgtcc tcgccatcgc gcttgaaccg ctcgtcgccc
     3001 tggacatgga gcacgacgta caggtggccg gtggtgccgc ccggcgcggt ctcgcccttg
     3061 ccggcgagcc gcagggtctg gccgtcgtcg acgccggccg gcacggtgac gctgagcgtg
     3121 gaggtctcgg ggcgcaggcc gcggccgcgg cagtcgtcgc acgggtcctt gatcgacttg
     3181 ccggcgccgc ggcagtgcgg acacgtggtc tgcaccatga agaagccctg ggcgtggacg
     3241 acctggccct tgccctggca ggtccggcag atctcgggct tgctgcccgg gcgagcgccg
     3301 ttgccaccgc aggtggcgca ggcgatctcg cggctgacct tgacgtcctt ggtgatgccc
     3361 cagaccgcct cggcgaacgt caggttcaga tcgacgcgca ggtcggcgcc gcgcggctgg
     3421 cggcggccgg cgccgcggcc tccgaagaag tcgccgaaga tgtcgccgaa cgccgacagg
     3481 atgtcctcga cgttggtgta cccgccggcg ttgccgttga cgctgcggat gccgtcgaac
     3541 ccgaaccggt cgtagcgctg gcgctgctcg tcgtcggaca ggatcgagta ggcgttcgac
     3601 gcttccttga acttctcctc ggcgtccttg tcccccgggt tgtgatccgg gtggtacttc
     3661 ttggcgaggc ggtagtacgc cttcttgagc tcggaaggac tcacctgccg atcgacttca
     3721 aggacttcgt agagatcgcg catggcgtgg agaatcagga ccttgggtaa ttcgggatgt
     3781 gccaggtgtc aaggtatggt caggccgtga tccggcccgc ggacctggcg cgcggcgccc
     3841 ccgacgccgc ggatgcgctc cgtcgcgccg agcggctgtt cgacgccgcg gcgcaaagcg
     3901 cgttggatct cgctgggttg ggcgatccgg cgcagcggat cgccacactt tgctgccagt
     3961 cggcgcccta cctcgccacg ctcctgaccc gggatcctca ccgtctgggg agagtggcgt
     4021 ccgatccata cctgcgccgg gaaaaaccgc gcgaggtcct tgtagcggag gtcacagcgc
     4081 gggccgcgcc ggcccggacc ccggccgagc tgcaagcggc gctccggagc gtgcgggcgg
     4141 acgagctcgt gcggctcggg gtccgcgagc tcgagctcgg gctcgacacc gaggtcggcc
     4201 gcgagctggc ccggctggcc gacgcctgct tcgacgccgc gatcgggttc tgggaccgcg
     4261 cgctgcgggc gcgctacggg gcgccgcgct a
//
LOCUS       DASVOR010001698         4168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_46979,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001698 DASVOR010000000
VERSION     DASVOR010001698.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46979"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1400
                     /locus_tag="VF516_31715"
     CDS             <1..1400
                     /locus_tag="VF516_31715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HEX8112357.1"
                     /translation="VGARDLAYVIYTSGSTGRPKGVMVDHGPIANLVEAQRILFGVRR
                     DSQVLQFANLGFDASVSEIFVTLGNGACLHLASPEQMAPGQPLIDILRRRRIHLVTLP
                     ASVLASLPPAELPDLATVVSAGEACSSAVVQRWTQHTALVNAYGPTEGCVCATGTQLT
                     ADDARRDPPIGPPIRNVRVYIVDPHGEPVPVGVTGELCIGGAQVARGYLDQPAQTAAS
                     FVPDPFSGEPGARLYRTGDLARYRADGQIEFLGRRDHQIKLRGFRIEPGEIESRLLRC
                     DGVREALALVRNEQLVAYVVGERVDVDAARQALQGALPRYMVPDQLVALPAFPLNANG
                     KVHRDLLPDPRTATPAAEPRPPGTPIEQSIAEIWKEILTIDRVGLDDSFIGLGGHSLL
                     AIQTAGRIEKRLGVRVPLEVLFSGKNLESLARDVEALLVPDGGAADPEVLLDELEQLG
                     EDELADLLDPEEDAR"
     gene            1397..>4168
                     /locus_tag="VF516_31720"
     CDS             1397..>4168
                     /locus_tag="VF516_31720"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX8112358.1"
                     /translation="MKILLIQLIDYLYPFAGAHRSNRLLMEGLAARGHDCVVYAPGYE
                     SIKPRPDEREILRRTLADKGCAVLREDPDFVVYRHRGVESHAIVSEFTLDVYPRFHAA
                     LRRLIEDFGPDVVLVSEDQTGLFHGAALDAAGERVIYLVHSQATLPFGPAAFARDDGK
                     ATLYRRTPCILTVSQYVRDYIARWGGCASEVVPFPVYGEPDPAVQPSFDRGAVTLVNA
                     SPLKGLSIFLGLAERFPDVPFATVATWDTEERRQITDLPNVTVLPPRERIDEIFAQAR
                     VLLVPSLWGEAFGLVIVEAMLRGLPVLASDVGGIPEAKLGVPYVLPINPIVDYQQPDL
                     SARPIPVIPEQDLGPWQDALARVLRDRDHYEALVEQSRTAARAFVGSLGYERFEQIFE
                     RVLGRQRKGAPVSRRASIASQLAALSPDQRAAVAARLSKRKQASRATSIPSLPRHRDG
                     RADDFPLSFAQLRFWLLDLFRRDGYSVAIAPYALTGPFRADWLAASLADLIERHETLR
                     TTFPIVDGEPVQRVHASMPVPLRQEDLRELPAGAQEERLQAIFREEDERGFDIAAECL
                     WRACLVRLTDERHLLILVLHHMIYDGWSTGILLGEMTALYRARCLGEPLRLEPIAVQY
                     ADFAAWQRQRLSGPSLEPLLAFWRSRLQDLPAALSLPIDRPRPALLGFRGMHLPVAFS
                     PALAQRVRRLARRENASLFIVLLAAFKVLLHRYSGQQDLVIGTPTAGRDRSELEAIFG
                     NFVNMLVLRTRLEPQASFLDLVGRVRHGALEGYEHQEMPFEKLVEALAPARDPSGHPI
                     FQVIFALNRRPDKEPTFHGVETTWYPPPRKPARVDLALDLWDEPDGQVTGIIEYNVDL
                     FEHATIERLRDHFLTLVDSLVENPELPVGRAPMLLADERRRFLPGSAPPRWTLPEGGV
                     HRDVSRWAER"
BASE COUNT          551 a         1578 c         1428 g          611 t
ORIGIN      
        1 gcgtgggcgc ccgcgacctc gcctacgtca tctacacgtc gggctccacc ggccggccga
       61 agggcgtgat ggtcgatcac gggccgatcg ccaacctggt cgaggcccag cgcatcctgt
      121 tcggagtccg ccgcgacagc caggtgctgc agttcgccaa cctcggcttc gacgcctcgg
      181 tgtcggagat cttcgtcacc ctcggcaacg gcgcctgcct gcacctcgcc agcccggagc
      241 agatggcgcc cggccagccg ctgatcgaca tcctccggcg ccgccgcatc cacctggtga
      301 cgctgccggc gtcggtgctc gccagcctgc cgcccgccga gctgcccgac ctcgcgaccg
      361 tggtgagcgc cggcgaggcc tgctcgtcgg cggtggtcca gcgctggacc cagcacaccg
      421 ctctggtcaa cgcctacggg ccgaccgagg gctgcgtgtg cgccaccggc acgcagctga
      481 cggccgacga tgcccggcgc gatccaccga tcggcccgcc gatccgcaac gtccgggtct
      541 acatcgtcga cccccatggc gagccggtgc cggtcggcgt caccggcgag ctgtgcatcg
      601 gcggcgccca ggtcgcgcgc ggctacctcg accagccggc ccagacggcg gcgagcttcg
      661 tgcccgaccc gttcagcggc gagcccggcg cccggctcta ccgcaccggg gatctggcac
      721 gctaccgggc cgacggacag atcgagttcc tcggccggcg cgaccaccag atcaagctgc
      781 gcgggttccg catcgagccc ggcgagatcg agagtcgcct gctgcgctgt gacggcgtgc
      841 gcgaggcgct ggccctggtg cgcaacgagc agctcgtcgc gtacgtggtc ggcgagcgcg
      901 tggacgtcga cgccgcgagg caggcgctgc agggcgcgct gccgcgctac atggtgcccg
      961 atcagctggt cgcgctgccg gcgtttccgc tgaacgccaa cggcaaggtc caccgcgacc
     1021 tgcttccgga tccgaggacc gcgacgcccg ccgccgagcc gcggccgccg ggcacgccga
     1081 tcgagcagag catcgccgag atctggaagg agatcctgac gatcgatcgg gtcggcctcg
     1141 acgacagctt catcggcctc ggcggccact cgctgctcgc gatccagacc gccggccgca
     1201 tcgagaagcg gctgggcgtg cgggtcccgc tcgaggtcct gttctcgggc aagaacctcg
     1261 agagcctcgc gcgggacgtc gaggcgctgc tggtccccga cggcggcgct gccgaccccg
     1321 aggtgctgct cgacgagctg gagcagctcg gcgaggacga gctggccgac ctcctcgacc
     1381 ccgaagagga cgcgcgatga agatcctgct gatccagctc atcgactacc tgtacccgtt
     1441 cgccggtgcg caccggtcca accgcctgct gatggagggc ctggccgcgc gcggccatga
     1501 ctgcgtcgtt tacgcgccgg gctacgaatc gatcaagccg cggccggacg agcgcgagat
     1561 cctgcgccgc acgctcgccg acaagggctg cgcggtgctg cgcgaggacc cggacttcgt
     1621 cgtctaccgc catcgcgggg tcgagtccca tgcgatcgtc tcggagttca cgctcgacgt
     1681 ctatccgcgg ttccacgccg ccctgcggcg cctgatcgag gacttcggtc ccgacgtcgt
     1741 cctggtctcc gaggatcaga cgggcctgtt ccacggcgcc gccctcgacg ccgccggcga
     1801 gcgcgtgatc tatctggtcc attcccaggc gacgctgccg ttcggccccg cggcgtttgc
     1861 cagggacgac ggcaaggcca cgctctaccg gcgcacgccg tgcatcctca ccgtcagcca
     1921 gtacgtgcgc gactacatcg cccgctgggg cggctgcgcg tcggaggtgg tgccgttccc
     1981 ggtctacggc gagcccgatc cggcggtgca accgtcgttc gaccgcggcg ccgtcaccct
     2041 cgtcaacgcc tcgccgctca agggcctgtc gatcttcctc ggcctggcgg agcggtttcc
     2101 cgacgtgccg ttcgccaccg tcgccacgtg ggacaccgag gagcggcgcc agatcaccga
     2161 cctgccgaac gtcaccgtgc tcccgccgcg ggagcgcatc gacgagatct tcgcccaggc
     2221 ccgcgtgctg ctcgtaccgt cgctgtgggg cgaggccttc gggctcgtca tcgtcgaggc
     2281 gatgctgcgc ggcctgccgg tcctggcgag cgacgtcggc ggcattcccg aggccaagct
     2341 cggcgtgccg tacgtgctgc cgatcaaccc gatcgtcgac taccagcagc ccgatctgtc
     2401 cgcccggccg atcccggtca tccccgagca ggatctcggc ccgtggcagg acgcgctggc
     2461 ccgcgtgctc cgcgaccgcg accactacga ggccctggtc gagcagtcgc gcacggcggc
     2521 gcgcgccttc gtcggcagcc tcggctacga gcgcttcgag cagatcttcg agcgcgtgct
     2581 cggccggcaa cgcaagggcg ctccggtatc gcggcgggcc tcgatcgcca gccagctggc
     2641 cgcgctgtcg ccggatcaac gcgcagccgt cgcggcgcgg ctctcgaagc gcaagcaggc
     2701 cagccgcgcc acctcgatcc cgagcctgcc gcggcaccgc gacggccggg ccgacgactt
     2761 cccgctgtcg ttcgcgcagc tgcggttctg gctgctcgac ctgttccgcc gcgacggcta
     2821 cagcgtggcg atcgcgccct acgcgctgac cggtccgttc cgtgccgact ggctcgcggc
     2881 gagcctcgcg gacctgatcg agcgccacga gacgctgcgg accacgttcc cgatcgtcga
     2941 cggcgagccc gtgcagcggg tccacgcgtc gatgcccgtg ccgctgcggc aggaggacct
     3001 ccgggagctt ccggccggcg cgcaggagga gcgcctgcag gcgatcttcc gcgaggagga
     3061 cgagcgcggc ttcgacatcg ccgccgagtg cctgtggcgc gcctgcctgg tgcgcctcac
     3121 cgacgagcgc cacctgctca tcctcgtcct ccaccacatg atctacgacg gctggtcgac
     3181 ggggatcttg ctgggcgaga tgaccgcgct gtaccgcgcc cgctgcctcg gcgagcccct
     3241 ccgcctggag ccgatcgccg tccagtacgc ggacttcgcc gcgtggcagc gccagcgcct
     3301 gtcggggccc tcgctcgagc ccctgctggc gttctggcgc agccgcctgc aggatctacc
     3361 ggccgcgctg agcttgccga tcgaccggcc gcggccagcg ctcctggggt ttcgcggcat
     3421 gcacctgccg gtggcgttct cccccgcgct ggcgcagcgc gtgcgccgcc tggcgaggcg
     3481 cgagaacgcg tcgctgttca tcgtcctgct cgcggcgttc aaggtgctgc tccatcgcta
     3541 cagcggccag caggatctgg tcatcggcac gccgaccgcg ggccgcgacc gcagcgagct
     3601 cgaagcgatc ttcggcaact tcgtgaacat gctcgtcctg cgcacccggc tcgagcccca
     3661 ggcgagcttc ctggatctgg tcggccgcgt gcggcacggc gcgctggaag gctacgagca
     3721 ccaggagatg ccgttcgaga agctggtcga ggcgctggcg ccggcgcgcg atccgagcgg
     3781 ccatccgatc tttcaggtca tcttcgcgct caaccgacgg ccggacaagg agcccacctt
     3841 ccacggcgtc gagaccacct ggtacccgcc gccgcgcaag ccggcgcgcg tcgacctggc
     3901 gctcgatctg tgggacgagc ccgacggtca ggtgaccggc atcatcgagt acaacgtcga
     3961 cctgttcgag cacgccacca tcgagcggct gcgcgaccac ttcctcaccc tcgtcgacag
     4021 cctggtcgag aatcccgagc tcccggtcgg ccgggcgccg atgctcctgg ccgacgagcg
     4081 gcggcggttt ctgcccggga gcgcgccgcc gcgctggacg ctccccgagg gcggcgtcca
     4141 ccgggacgtg tcgcgctggg ccgagcgc
//
LOCUS       DASVOR010001699         3242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_514235,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001699 DASVOR010000000
VERSION     DASVOR010001699.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514235"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..2639)
                     /locus_tag="VF516_31725"
     CDS             complement(96..2639)
                     /locus_tag="VF516_31725"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8112359.1"
                     /translation="MRGWLISCLLLVAGAARADCPRTGKTIPVAHGSVEVDGRLDDPA
                     WATACFIDDFEQKQPAYGAQPTRRVTTAVAIDRDTLYVAARMWSAGPGDIDDALTQRD
                     DTGQAERFIVSIDPSHTRRVAYSFAVTAAGVRADWIHTDDTEGARDATWNPVWRARTA
                     IQPDGWTAELAMPLSQLRLPAAPQTSWGINFNWYIPHRNEDVFWRAVPPDRTAWASWF
                     GELTDLPRIDPGPGLELLPYAAHRLTINESPSDVLAHRFDGGIDAGLDVKFRPLPGLT
                     VAATVNPDFAQVDVDPAFVNLTAFEVQLPEQRPFFVANAPLFADAGGNYFYRRRIGGL
                     PHTLPTADEIDLPTQVRILGAAAAGGFLADRTQIAALGAVTGSADADAVIGGATQRLA
                     IAPLTGWAAARIEHQVGASVLGATATAVERDLGGSPLARLLARTALVGGGDARLRTAD
                     GTYEAFLYAGLSSVFGTPEAIATVEQSSAHYFQRPGAGYLHVDTDARRLIGWHAGAVG
                     TKRAGEWQGTASANVESPGFELNDLGVLQSADDIDLSTDLRHTVTTPGAPVHQWDAGV
                     GASASWNFGGLRKPVDLRASGHITSHAFNSASIALDVTTPGGSDDLTRGGPVMRTGWA
                     ESVKLTANTPRDRAQQLSATLVGQLSPTLQQGIMASASLASRVMPSLRLDLTPSFTWI
                     ESQRQYVTTRTDAGDGKHYLFGQIHRKEAALELRATWSLSPELVVTVYAQPFASVGRY
                     DQLGELAAAGSGDIRWYTATSHAGAMRAIADGATGFSINEPDFTVLSLRSTAVLRWEL
                     SPGSTLFIVWQQTRQGSTPRAQPLHVVAPDVVTQPGLHSFAIKLSYWFG"
     gene            2814..>3242
                     /locus_tag="VF516_31730"
     CDS             2814..>3242
                     /locus_tag="VF516_31730"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX8112360.1"
                     /translation="MRRAVIAIAAALASAPSAAHAFCGFYVTGSDQQLLADATQVVLM
                     RKGTRTVLSMQNNYKGPPEAFAMVIPVPVVLHEQDVKTLPAEVFASVERMGAPRLVEY
                     WEQDPCRGPVLYDAMPAPAPGGPTLRAIAPRKPGDLGVTVE"
BASE COUNT          448 a         1137 c         1224 g          433 t
ORIGIN      
        1 atcgctgcgg cacaggttct ggcgagtctc atgggtcgaa gtaatgcatc tcgcgtgccg
       61 ccgcagtgcg gcgagccgtc gcagaggcca ttccgtcaac cgaaccagta agacagcttg
      121 atcgcaaagc tatgcaggcc gggctgcgtc acgacgtccg gcgccacgac atgcagcggt
      181 tgcgcgcgcg gggtgctgcc ctgccgggtt tgctgccaca cgatgaacag cgtactgccg
      241 ggcgacagct cccagcgcag caccgcggtg gagcgcagcg acagcacggt gaagtccggt
      301 tcattgatcg agaacccggt cgcgccatcc gcgatcgcgc gcatcgcacc ggcgtgcgag
      361 gtcgcggtgt accaccggat gtcaccggag ccggccgcgg ccagctcgcc gagctgatcg
      421 tagcggccga ccgacgcgaa cggctgggcg tacaccgtca cgaccagctc gggcgacagc
      481 gaccacgtcg cgcgcagctc cagcgcggcc tccttgcggt ggatctggcc gaacaggtag
      541 tgcttgccgt cgcccgcgtc ggtcctggtg gtgacgtact ggcgctggga ctcgatccag
      601 gtgaacgacg gcgtcaggtc gagccgcagc gagggcatca cgcgcgaggc caggctcgcc
      661 gacgccatga tgccctgctg cagggtcggg ctgagctggc cgaccagcgt cgccgacagc
      721 tgctgggcgc ggtcgcgcgg cgtattcgcg gtcagcttca ccgactcggc ccagccggtg
      781 cgcatcaccg ggccgccgcg ggtcaggtcg tcggacccgc ccggcgtggt gacgtcgagc
      841 gcgatcgacg ccgagttgaa cgcgtgcgac gtgatgtggc cgctggcccg cagatcgacc
      901 ggcttgcgca gcccgccgaa gttccacgac gcgctcgcgc ccacgccggc gtcccactgg
      961 tgcaccggcg cgccgggcgt ggtgaccgtg tggcgcaggt cggtcgacag gtcgatgtcg
     1021 tcggcggact gcagcacgcc gaggtcgttg agctcgaacc ccggcgactc gacgttggcc
     1081 gatgcggtgc cctgccactc gcctgcgcgc ttggtgccga ccgccccggc gtgccagccg
     1141 atcagccggc gcgcgtcggt gtcgacgtgg aggtagcccg cgccggggcg ctggaagtag
     1201 tgcgccgagc tctgctcgac ggtggcgatc gcctccgggg tgccgaacac cgagctcagg
     1261 ccggcgtaca ggaaggcctc gtaggtgccg tccgcggtgc gcagccgggc gtcgccgccg
     1321 ccgaccagcg cggtccgcgc cagcagccgg gcgagcggcg agccgccgag gtcgcgctcg
     1381 acggcggtcg cggtcgcacc gagcaccgag gcgccgacct ggtgctcgat ccgtgcggcg
     1441 gcccacccgg tcagcggtgc gatcgcgagc cgctgggtcg cgccgccgat caccgcgtcg
     1501 gcgtcggccg agccggtcac cgcgccgagc gcggcgatct gggtgcgatc ggcgaggaag
     1561 ccgccggcgg ccgccgcgcc gaggatccgg acctgggtcg gcagatcgat ctcgtcggcg
     1621 gtcggcaggg tgtggggcag gccgccgatc cggcggcggt agaagtagtt gccgccggcg
     1681 tcggcgaaca gcggcgcgtt ggcgacgaag aacggccgct gctcgggtag ctggacctcg
     1741 aacgcggtga ggttgacgaa cgccgggtcg acgtcgacct gcgcgaagtc cgggttgacg
     1801 gtcgccgcga cggtgagccc gggcagcggg cggaacttga cgtcgagccc ggcatcgatg
     1861 ccgccatcga agcggtgggc cagcacgtcg ctcggcgact cgttgatggt cagccggtgc
     1921 gcggcgtagg gcaagagctc caggccgggg ccggggtcga tccgcggcag gtcggtgagc
     1981 tcgccgaacc agctcgccca cgcggtgcgg tcgggcggca cggcgcgcca gaacacgtcc
     2041 tcgttgcggt gcgggatgta ccagttgaag ttgatccccc agctggtctg cggcgccgcg
     2101 ggcaaccgga gctgcgacag cggcatcgcg agctcggcgg tccacccgtc gggctggatc
     2161 gcggtccggg cccgccacac cgggttccag gtcgcgtcgc gggcgccctc ggtgtcgtcg
     2221 gtgtggatcc agtcggcgcg gaccccggcc gcggtgaccg cgaagctgta cgccacccgc
     2281 cgggtgtgcg acggatcgat cgacacgatg aaccgctcgg cctggccggt gtcgtcccgc
     2341 tgggtcagcg cgtcgtcgat gtcgcccggt ccggccgacc acatccgcgc cgcgacgtag
     2401 agcgtgtcac ggtcgatcgc gaccgcggtc gtcacccggc gggtcggctg cgcgccgtac
     2461 gccggctgct tctgctcgaa gtcgtcgatg aagcacgcgg tcgcccacgc cggatcgtcg
     2521 agcctgccgt cgacctcgac cgagccgtgc gcgaccggga tggtcttgcc ggtgcgcggg
     2581 cagtcggcgc gggccgcccc cgcgacgagg agcaggcacg agatcagcca gccgcgcatc
     2641 cgcgggcgaa tgtacagcgc cgggccaccg cgcaaagccc gccgcgccgg aatccgtcga
     2701 accgcacgcg cggtgggccg gtcggcgcgc cgcgccgcgc cagcgcagac cgcgcagcag
     2761 ccgttgatcg ccagcggcga acgcggtttg ctcatggtcg gaggagcatc gccatgagga
     2821 gagccgtcat cgccatcgcg gccgcgctgg ccagcgcgcc gagcgccgcc cacgcgttct
     2881 gcgggttcta cgtcaccggc agcgatcagc agctgctcgc cgacgccacg caggtcgtgc
     2941 tgatgcgcaa gggcacgcgc acggtgctgt cgatgcagaa caactacaag ggaccacccg
     3001 aggcgttcgc gatggtgatc ccggtgccgg tcgtgctcca cgagcaggac gtcaagacgc
     3061 tgccggccga ggtgttcgcc agcgtcgagc gcatgggcgc gccgcgcctg gtcgagtact
     3121 gggagcagga cccatgccgg gggcccgtgc tctatgacgc gatgccggcg cccgcgcccg
     3181 ggggcccgac gcttcgggcg atcgccccca ggaagcccgg cgacctcggc gtcaccgtcg
     3241 ag
//
LOCUS       DASVOR010001700        10408 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_425861,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001700 DASVOR010000000
VERSION     DASVOR010001700.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10408
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425861"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="VF516_31735"
     CDS             <1..415
                     /locus_tag="VF516_31735"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX8112361.1"
                     /translation="WFEIPVLSFLDDRLTVIYQRRYIESAQRFAGAPRLDDRQRAALD
                     AFDAVLDDPALHLEMDLEPGDVQLVHNHQLLHDRTAFVDDPDPTRRRHLLRLWLCPPV
                     GRRLPPVFAQRYGSIEIGRRGGVVPRGSPVITLSP"
     gene            complement(452..1162)
                     /locus_tag="VF516_31740"
     CDS             complement(452..1162)
                     /locus_tag="VF516_31740"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8112362.1"
                     /translation="MCAIDWSKSRQAVSGGADCGKVEGVLLVEIFPAGPLLCNCTVLA
                     CADTKDAVVVDPGGETRRIAEIVLHYDLKVRAIIHTHGHADHMYCTDDVKRQHGGEIC
                     LHRDDAALYDAFHRQCAVFGLPRRGVSPVDRFVEHGDRVALGEHAIEVIHTPGHTPGS
                     VCFEVVDGTRTILFAGDTLLRRSVGRTDLPGGDTGTLHRSIRERLYTRNPGAVVVPGH
                     GPSTILGEELKGNPYVRP"
     gene            complement(1455..1991)
                     /locus_tag="VF516_31745"
     CDS             complement(1455..1991)
                     /locus_tag="VF516_31745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112363.1"
                     /translation="MARQLIRMLAVVVGLAVMVFNPEYGVFSGPSFGAAELRAAVEGT
                     WRLTVRGEDGTSRSVTFRLAQGSEVEGKHAAVHALVRPAAACGRRSLVKPAGACVDVT
                     RMPLEVTLVADGGERRQAEPGEFVVDGSAFHQGELEVRVAELRVRASVTPAGEVQDVS
                     SVGPARRALPSTLVRIAR"
     gene            2108..2204
                     /locus_tag="VF516_31750"
     tRNA            2108..2204
                     /locus_tag="VF516_31750"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2143..2145,aa:Sec,seq:tca)
     gene            complement(2249..6019)
                     /locus_tag="VF516_31755"
     CDS             complement(2249..6019)
                     /locus_tag="VF516_31755"
                     /inference="COORDINATES: protein motif:HMM:NF019237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8112364.1"
                     /translation="MTERETQQAGRFDAFISYSRRDIAFARKLQSVLESYVPPIGLGL
                     EPRSLRVFRDESDIQGSRYYNSIDDHLRQSRRLVLICSPSARQSRFVDDELTRFLTYR
                     RPDDIIPVLLSGKPNGETKVSEHWAFPDVLMDHLGEPRALEMREFDPRIDEFDGQRWV
                     SSWYSLLADLFGLERRVIEERDSRRRFIEGSVRAKDLWYRSYDARIAGYWLESLHRLA
                     DAHEQAPPGAVKRNLRAALFHALPEVEASNIWQGDGLLIGGTLSPGGTHLVVWSDRCR
                     ALGFDMATGACLRSDVTHERAIQTGRFLTESCAATTDLAGRVNIFDLRTNDAPVSLER
                     PSPVLWLMSSDTEPLVATLNRSGPAISRALDPSEELVTLWDWRRAALLVECVVAGWPR
                     CFFCNRGQQLAIVVNGRLLLARADGKVLELKLPEDASVMSFGPEAVLIRQPSRLHVMR
                     FDASGQPAIAAPLVLDDADAVKQCVADQQGRFVIGLTVDERIIVIDTLKQSVRVIAQV
                     HGLGATELVYEGCGVITVHGIAGKAAYTLGGALLWKQDARATSDPLLAAWLQEPSRAP
                     HGNATPFLPYPAASLFELGWQYSVMNGKSLSRSIGGIALGDIRVEGIGGSSGEQTARQ
                     RAASFSSDQRIRFWKLRTARPSVPRTTYGQVAFSSFAFGKIRLGFASEDGLVHLLDPF
                     LGALVHSVAQTTPALWGVLLDANDTTVFGRAGDTDVVRWPSNDGRPEPQRIGFPIPIT
                     QLSTAEPFDRAGALLADGSLHIVRLSEDLVVEGILRHQGNPLGNVQFQFSADGCRLVT
                     WAQAGPIRMWDVSSRQELCSPIAGTGSNWGAWFNSSGSRVLTWNAENECIVKDIASGR
                     ELTIRHFGNDGTFLPFWQAGIAAARFSVNGDLVFTASSDRTVGVWDAETGERILPLLE
                     HRSGIDALLPSYDDRSLLVSAGDRVSLWDLRTGKRAFKDVIFQGGTPAICWGGHEERW
                     VGFTPDGTIRVLDRDTGLPLAEFAHPGGQVSRVTLNADDTEIVSCSKDGIARVWKIDP
                     DVEPTRWKDAVELTTASRYDKDSFEIVALDDGEFRERSGRLAGFADAPDVRSRPVSCA
                     CTRDALQRLRIRPTGLLGEKALRELLRDQRLFDGDLNPAPGIAPAVEDRGNDIVAQPD
                     RDLLWQRTGSPAAVLFTEALKFVSDLNAARFGGFADWRLPSLLEAMSLMTSEGLGENL
                     HIPDVFDRKQTWIWTSSMGDAGAVWTASYGWARCRSCDPTLPAFVRAVRWAN"
     gene            complement(6298..6801)
                     /locus_tag="VF516_31760"
     CDS             complement(6298..6801)
                     /locus_tag="VF516_31760"
                     /inference="COORDINATES: protein motif:HMM:NF020909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1993 domain-containing protein"
                     /protein_id="HEX8112365.1"
                     /translation="MSLHLIVLEMKKLLGNLDTCLEKAAGHAAAKKYDPSVLLQSRLA
                     PDMFPLIRQIQIACDQAKYAASRAAGKDAPSHADTEQTLDEIRARIATVIAYLGEFSA
                     ADFDGASARSISLPRWEGTSMTATDYVIEHAMPNFFFHYTTTYAILRHNGVDIGKRDY
                     LGALTFR"
     gene            6974..7588
                     /locus_tag="VF516_31765"
     CDS             6974..7588
                     /locus_tag="VF516_31765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112366.1"
                     /translation="MLIASIVAAAGTASALPNPDARFRPGANHHLGDDSFVARFGRLP
                     TVADDEHVRMKVHLEFVRAWLAARPATRPELAARRAELLGYLDDYIANGVTPRNTHLP
                     WRSAVFIDDAGAICAVGYLIERSVGRALPERIAGDLARVLAAYTIGYLEWPHIDGRFE
                     RVFATMAGRMRWEWGGSDPEADGTNPLLNDDEPSHRLTPRGFGR"
     gene            complement(7601..8302)
                     /locus_tag="VF516_31770"
     CDS             complement(7601..8302)
                     /locus_tag="VF516_31770"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HEX8112367.1"
                     /translation="MSQLRHLAGSVAAARSEQHLAALVVDDGPGLFEADSIGITFFGE
                     TVSSSAIEVTRGSEPFVDQYQRHLCSVDPLLQCLLEQHRSVRDLDVRSRKHWLADPLY
                     TDIAAPFGMVRCMQGPILADGRVTGVIFVGRRRDHLFSMEDVLTLSSVSAHVSVQLAR
                     LRSMPRALERLRSVLTEREVEVSLLVAKGLTNEQIGHVLGVSANAIKGTLARAFRKLD
                     IESRAELVATLFDGL"
     gene            8607..9443
                     /locus_tag="VF516_31775"
     CDS             8607..9443
                     /locus_tag="VF516_31775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112368.1"
                     /translation="MSIILGCNADIYSAFIAAIPGSAGCRSYQDDILYEPGDVPATFP
                     GAPGSRVVASIRPYPEALLFGSLDRAILAMLRDGAARFTAPQLTAWHEAGHLYQDLPY
                     LTPAVVRLVHAKMQDLCNQVPGVEYGCVVHGDISTMDKWIPYAPDALDWYGIDVYWND
                     PFDFSIYDQLKAYLDGYRTLAQHRTGLLYPRINVCAANSHDESRRPGLFKNVARWLHH
                     HGGSRMLTSYQDGDPSGGPWAPGDTDTINALKYIAASYVHPQAGSERRRASFWEPKPR
                     LS"
     gene            9518..>10408
                     /locus_tag="VF516_31780"
     CDS             9518..>10408
                     /locus_tag="VF516_31780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017404459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8112369.1"
                     /translation="MNKLIDDITRLERPTPSDSRVAEFGSDVVAETLRALEIPYIAVN
                     PGASYRGLHDSIVNLLGNERPQMLLCLHEESAVAIAHGYAKVTGRAMAVAVHSNVGLL
                     HATMAIFNAWCDRMPVLVLGATGPVDATRRRPWIDWIHTAADQGAVVRHYTKWDDQPA
                     SAGAARESLLRAVWLANSPPQAPVYVNLDAEMQEARLPAPLPPIDARRFLPRVVAGPP
                     ADVVPRAVERLTAARRPLILAGRVSRDLAAWNRRVELAEALGARVATALKVGAAFPTD
                     HPLHAGARTTLATDDVPAASR"
BASE COUNT         1691 a         3695 c         3306 g         1716 t
ORIGIN      
        1 gtggttcgag atccccgtgc tgtcgttcct cgacgaccgg ctgaccgtga tctaccagcg
       61 gcgctacatc gagtccgccc agcggttcgc cggcgcgccc cggctcgacg accgccagcg
      121 cgccgcgctc gacgcgttcg acgccgtgct cgacgacccc gcgctccacc tcgagatgga
      181 cctcgagccc ggcgacgtcc agctcgtcca caaccaccag ctgctccacg accgcaccgc
      241 gttcgtcgat gaccccgatc ccacccgccg ccgccacctc ctgcggctgt ggctgtgccc
      301 gccggtcggc cgccggctgc ccccggtgtt cgcccagcgc tacggctcga tcgagatcgg
      361 ccggcgcggc ggcgtcgtgc cccgtggttc gcctgtgatc acgctctcgc cgtgacgggc
      421 gatgcggatg tcgcggtgac cgccgggcgc gtcacggccg gacgtacggg ttgcccttga
      481 gctcctcgcc gaggatcgtc gacggcccat gcccgggcac gaccaccgcg cccgggttgc
      541 gcgtgtacag ccgctcgcgg atcgagcggt gcagcgtccc ggtgtcgccg cccggcaggt
      601 cggtgcgccc gaccgagcgc cggagcagcg tgtcgcccgc gaacaggatc gtgcgcgtcc
      661 cgtcgacgac ctcgaagcac accgagcccg gcgtgtgccc cggcgtgtgg atcacctcga
      721 tcgcgtgctc gccgagcgcg acccggtcgc cgtgctcgac gaagcggtcc accggcgaca
      781 ccccgcgccg cggcagcccg aagaccgcgc actggcgatg gaacgcgtcg tacagggccg
      841 cgtcgtcgcg gtgcaggcag atctcgccgc cgtgctgccg cttgacgtcg tcggtgcaat
      901 acatgtggtc ggcgtggccg tgggtgtgga tgatcgcgcg gaccttcaga tcgtagtgca
      961 ggacgatctc cgcgatccgc cgggtctcgc cgccgggatc gaccacgacg gcgtccttgg
     1021 tgtccgcgca cgcgagcacc gtgcagttgc acagcaacgg cccggccggg aagatctcga
     1081 cgagcagcac gccctcgacc ttgccacagt ccgcgccgcc cgacaccgct tgccgcgatt
     1141 tgctccagtc aatggcgcac atttgcaaca gatcgtcatt ccgtatctgc agatgtcagg
     1201 tgggtgttcc gccggccgtc gcacgccccg cagcgcaatt ttcttccccg ggcgcgcccg
     1261 tcgaggacct cgtgcgtcaa cgcccgtcga gatcgctgta acgcccgatc cactgcgtgc
     1321 cgaaaccgag cgcgcgtctc cagcgtggac ggatttctcg ctgtcctcgg agatcggacg
     1381 tcgatatttc cggccatgct gcgccggtgg cgcacggcct gcgcccgccg cccacgccgt
     1441 gcaattcgtg cagctcagcg cgcgatccgc accagggtag acggaagtgc tcgccgcgcg
     1501 ggcccgacgc tcgacacgtc ctgcacctcg ccggccggcg tgacactcgc gcgcacccgg
     1561 agctcggcga cccggacctc cagttccccc tgatgaaatg cgctaccgtc gaccacgaac
     1621 tcccccggct ccgcctgtcg ccgctcgccg ccgtccgcca ccagcgtcac ctccagcggc
     1681 atccgcgtga cgtccacgca cgccccggcc ggcttgacca gcgaccgcct cccgcacgcc
     1741 gccgccggcc gcaccagcgc gtgcaccgcc gcatgcttgc cctcgacctc gctgccctgc
     1801 gcgagccgga acgtcacgct gcgcgaggtc ccgtcctcgc cccgcaccgt cagccgccac
     1861 gtcccctcca ccgccgcgcg cagctccgcc gcgccgaagc tcggcccgct gaacacgccg
     1921 tactccgggt tgaacaccat caccgcgagg ccgaccacca ccgccagcat ccggatcagc
     1981 tgtctcgcca tgctccttcc atgacacgcc gggttcgcca cctattgcag cgccccgtgc
     2041 tcccgcccgg ctcgtgtttt cccgccgcgg acttgcgccg cgccgcccgc ggtgtaacgt
     2101 cggcggtgga agcgcgtgcc ggactggtgc cgacctcggt cttcaaaacc gatggagcct
     2161 cgcgcgagcg acggctcgga gggttcgatt cccttgcgtt tccgcgatct tgtactgttg
     2221 aggcgaactg agacgtcttc cagggcctct agttggccca gcgcacggcc ctgacgaacg
     2281 ccggaagggt cgggtcgcac gaacggcacc gtgcccagcc gtagctcgct gtccagacag
     2341 cgcccgcgtc gcccatgctg gaagtccaga tccatgtctg ctttcgatcg aacacgtcgg
     2401 ggatgtgcag attctcgccg aggccttccg aggtcatgag cgacatggcc tcgagcaggc
     2461 tcgggagtct ccagtcggcg aacccaccga agcgcgccgc gttcagatcg ctcacgaact
     2521 tgagcgcttc ggtgaacagt accgcggccg gagatccggt gcgctgccac agcaggtctc
     2581 gatccggctg cgcgacgata tcgttgccgc ggtcctcgac cgctggtgca ataccaggtg
     2641 ccggattcag gtcgccatcg aacaaccgct ggtctcgaag cagctcgcga agcgcctttt
     2701 cgccgagcaa cccggtcggt cggatccgga gccgttgcaa cgcgtcccgc gtgcatgcac
     2761 agctcaccgg tcgtgatcgg acgtccggtg catcggcgaa gccagcgagg cgccccgacc
     2821 gctcgcgaaa ttctccatcg tcgagtgcga cgatctcgaa cgagtcctta tcgtagcggg
     2881 acgctgtcgt cagttccact gcgtccttcc atcgggtcgg ttccacatcg ggatcgattt
     2941 tccacacgcg cgcgatgccg tccttcgagc atgagacgat ctcggtgtcg tcagcattca
     3001 gcgtcacgcg gctaacctga ccacctgggt gggcgaactc tgcgagcggc aagccggtat
     3061 cccggtccag cacgcgaatc gttccgtctg gcgtgaagcc gacccagcgc tcctcatggc
     3121 ctccccagca gatcgcgggg gttcctcctt gaaagatcac atccttgaag gcgcgcttgc
     3181 ccgtacgtag atcccagagc gacacccggt caccggcgct gaccaagagc gaccgatcat
     3241 cgtagctcgg cagcaaggcg tcaatgccac ttcgatgttc gagcagcggc aagatgcgtt
     3301 cgccggtctc ggcgtcccat actccgacgg ttcgatcact gctcgcggtg aacacaagat
     3361 cgccgttgac gctgaaccga gccgcggcga tccccgcctg ccaaaatggc aaaaatgttc
     3421 catcattgcc gaaatgccgg atcgtcagct ctcggccact ggcgatatcc ttcacgatac
     3481 attcattctc agcattccat gtcaggacgc gagatccaga gctattgaac cacgcccccc
     3541 agttggatcc tgttccggcg atcggcgaac acagctcctg cctgctcgat acatcccaca
     3601 tacgtatcgg accggcctgg gcccacgtga cgagcctgca accgtcggcg ctgaactgga
     3661 actggacgtt tcccagaggg ttcccctggt gacgcaggat accctcgaca acgagatcct
     3721 ccgacagccg gacgatatgg agagagccat cggccagcaa ggctccggct cgatcgaagg
     3781 gctcggcggt cgacagctgg gtgatcggga tcgggaaccc gatgcgttgc ggctcggggc
     3841 ggccgtcgtt cgacggccac cgcacaacgt cggtatctcc ggcccggcca aatactgtcg
     3901 tatcattggc gtcgagcaac accccccata acgccggcgt tgtctgggcc accgagtgga
     3961 ccagtgcccc caaaaacggg tccagcaggt gaacgagccc gtcctcggac gcaaatccca
     4021 gtcggatctt accaaacgca aaactcgaga aggcgacctg cccgtaggtc gtgcgcggga
     4081 cgctcgggcg cgcagtccgc aacttccaga accggatccg ttggtcactc gaaaacgacg
     4141 ctgcccgttg tcgcgcggtc tgttcgccgc tcgatccgcc gatcccttcc accctgatat
     4201 cgcccagtgc gattccaccg atcgacctcg acagactctt tccgttcatg acagaatact
     4261 gccagccgag ctcgaagagg cttgctgctg gatacggaag gaagggcgtg gcgttgccat
     4321 gcggcgcacg cgatggttcc tgcagccagg cggccaagag ggggtcggag gtcgctcggg
     4381 catcctgctt ccagagcagc gcgcctccga gcgtatacgc ggccttgccg gcgataccgt
     4441 ggacggtgat cacgccgcac ccctcgtaga cgagctccgt cgctcccaag ccgtggacct
     4501 gcgcgatgac tcgtacgctt tgcttcagcg tgtcgatcac gatgatgcgc tcgtcgaccg
     4561 tcagcccgat gacgaatcgg ccctgctggt ccgccacgca ctgcttcacg gcgtccgcgt
     4621 cgtccagcac gagcggtgcc gcgatggcgg gctggcccga ggcgtcgaag cgcatcacgt
     4681 gaaggcggct cggttgtcga atgagcaccg cctccggacc gaacgacatc acggaggcgt
     4741 cctcgggcaa cttgagctcg agcaccttcc cgtccgcgcg cgcaagcaac agtcgtccat
     4801 tcaccacgat ggcgagctgc tgcccccggt tgcaaaagaa gcatctcggc catcccgcaa
     4861 ccacgcactc gacgagcaac gctgcccgcc gccagtccca gagcgtgacg agctcttccg
     4921 atgggtcgag cgcccgggag atcgcaggac cgctgcgatt caatgtcgcg actagcggct
     4981 cggtgtcgct cgacatcagc cacaagaccg ggcttggccg ctcgagcgag accggtgcgt
     5041 cgttggtcct gagatcgaag atattgaccc tacccgcgag gtccgtggtc gccgcgcagc
     5101 tctcggtcag gaaccttccg gtctggatcg cgcgctcgtg ggtcacgtcg ctgcgcaagc
     5161 acgccccggt ggccatatca aacccgagcg cccgacagcg atcgctccac accacgagat
     5221 gagtgcctcc tgggctgagc gtaccgccga tcagcaggcc atcgccctgc cagatattcg
     5281 aagcctcgac ctccggcaac gcgtggaaca gcgcggctcg caggttccgt ttcaccgctc
     5341 ccggtggcgc ctgttcgtgg gcatcggcga gccggtggag cgattccagc cagtagcccg
     5401 cgatgcgcgc gtcgtacgac cggtaccaca agtcctttgc gcgaacgctg ccctcgatga
     5461 acctgcgccg gctgtctcgc tcttcgatga cgcgccgctc gaggccgaac aggtctgcga
     5521 ggagcgaata ccaggaagac acccatcttt ggccatcgaa ctcatcaata cgcgggtcga
     5581 actctcgcat ctccagagct cgaggctcac cgaggtggtc catgagaaca tcagggaatg
     5641 cccagtgctc tgaaaccttg gtttctccgt ttggttttcc ggacaacaag acggggatga
     5701 tatcgtccgg gcgacggtag gtgagaaacc gagtcagctc gtcgtcgacg aatcgactct
     5761 gtcgggcact cggtgaacag atcaggacga gcctgcgcga ctgacgcagg tgatcatcga
     5821 tggaattata gtagcgtgat ccctgaatgt ctgactcgtc gcgaaatacg cgaaggctcc
     5881 gcggttcgag cccgaggccg atgggcggga cataactctc caggacggac tgcagctttc
     5941 gcgcaaaggc gatgtcgcgt ctactgtagc tgatgaaagc gtcaaaccgc cccgcttgct
     6001 gggtctctcg ttccgtcatg gctgcatcgg ctccgctgcc ccgttgctta acacagcacg
     6061 tgcatgcgtc ggcgatgtcc tgcgagggct gcagctcgcc ggagaactcg cgccggccga
     6121 ccgtcggtcc cgacctgcgg gtcggcatct gtcggtatcg cgagccttcg ggtggtgcgc
     6181 ctggctccta catgtagcga accaatcccg actcgctggc tacccgttcc acgctgaaga
     6241 tcggctccca cgtccaggag gccgcgcgca agaagctact gggttgaccc ggccggatca
     6301 gcgaaacgtg agggccccga ggtagtcgcg cttgccgatg tcgacgccgt tgtggcgcaa
     6361 gatcgcgtag gtggtcgtgt agtggaagaa gaagttgggc atcgcgtgct cgatgacgta
     6421 gtcggtcgcc gtcatcgacg tgccttccca gcgcggcagg ctgatggagc gagcgctcgc
     6481 gccgtcgaag tcggccgcgg agaactcgcc gaggtacgcg atcaccgtgg cgatccgcgc
     6541 gcggatctca tcgagggtct gctcggtgtc ggcgtgcgac ggggcgtcct tgccggcggc
     6601 gcgggacgcc gcgtacttgg cctggtcaca ggcaatctgg atctggcgga tcaacggaaa
     6661 catgtcgggc gcgagccggc tctgcaacag cacgctgggg tcgtacttct tggccgcggc
     6721 gtggcccgcg gccttctcga ggcaggtgtc gagattgccc agcagcttct tcatttcgag
     6781 cacgatgagg tggagcgaca tgcgcgatcc ctaccacggc gcttccgcga acgcgcggac
     6841 cggcgcgtgc ggaggtggcc ggcctggtga ccggggcttg cgatcgcgtc gggtggcacc
     6901 gtgaccggag tgcacggtga aggatttctg gcatgaaccg gcgcgctcga gcgtttcccg
     6961 gcgtatgcgc ctgatgctca tcgcatcgat cgtggccgcc gcgggcaccg cgtccgcgct
     7021 gcccaacccc gacgcccggt ttcgccccgg cgcgaaccac cacctcggcg acgacagctt
     7081 cgtcgcgcgg ttcgggcggc tgccgaccgt ggccgacgac gagcacgtgc ggatgaaggt
     7141 gcacctcgag ttcgtccgcg cgtggctcgc ggcgcggccg gcgacccggc ccgagctcgc
     7201 ggcgcggcgc gccgagctgc tgggctatct cgacgactac atcgcgaacg gcgtgacgcc
     7261 gaggaacacc cacctgccgt ggcgcagcgc ggtgttcatc gacgacgccg gcgcaatctg
     7321 cgccgtcggg tacctgatcg agcgctcggt cgggcgcgcg ctgcccgaga ggatcgccgg
     7381 cgacctcgcg cgcgtgctcg ccgcgtacac catcggctac ctcgagtggc cgcatatcga
     7441 cggccgcttc gagcgcgtgt tcgcgacgat ggccggccgg atgcgctggg agtggggcgg
     7501 gagtgacccc gaggcggacg gcacgaaccc gctgctcaac gacgacgagc cgtcccaccg
     7561 gctgactccg cgcgggtttg gtcggtgagc cggtcccgga tcacagaccg tcgaacaggg
     7621 tcgcgaccag ctccgcgcga gattcgatgt cgagcttgcg gaacgcgcgc gccagcgtgc
     7681 ccttgatggc attggcgctg acgccgagca cgtgaccgat ctgctcgttg gtcagaccct
     7741 tcgcgaccag caagctgacc tcgacctccc gttccgtgag cacgctgcgc aagcgctcca
     7801 gcgcgcgcgg catcgacctc agccgggcca gctgcacgga gacgtgggcg ctgacactcg
     7861 acagggtcag gacgtcttcc atcgaaaaca gatggtcgcg acgccgcccg acgaagatca
     7921 ccccggtcac gcgtccatcg gccaggatcg ggccctgcat acagcgaacc ataccgaatg
     7981 gcgccgcgat gtcggtgtac agcggatctg ccaaccagtg tttacgagat ctgacatcca
     8041 gatcgcgaac cgatctgtgc tgctccagca ggcattgcag caacgggtca accgagcaga
     8101 ggtgacgctg gtattgatcg acgaacggct ccgagccacg cgtgacctcg atcgcggacg
     8161 acgagacggt ctcgccgaag aacgtgatcc cgatgctgtc ggcctcgaac aggcctggac
     8221 cgtcgtccac gaccagcgcc gcgagatgct gttcgctcct ggcggctgca acggaccctg
     8281 ccagatgacg tagttggctc atggaggcta cccgttcagg tacttgtacc tggaatcctc
     8341 accagctaga tccgaagcat acaaacgtac acgagtttgc cagctgtagt acatcgctgc
     8401 gtgcggcgca tcgctccagc cgtcgtgatg ccgcgcaccg gcgctgattc gcatccgggc
     8461 cgcacgtccg gggtccgggg cgtgcgctgg cgcaccggcc ggggacgccg ggtcctggcg
     8521 cgtcgtggtc ggaccaggcc tctcgccccc gcgtcgacgc tctcgacgcg ccacgttcca
     8581 tgcgatcgaa cgacaggaga catggcatgt cgatcatcct gggctgcaat gcggacatct
     8641 attccgcgtt catcgctgcc atccctggga gtgcgggctg ccgcagctac caggacgaca
     8701 tcctctacga acccggtgac gtcccggcga ccttccccgg cgcgccgggc tccagggtcg
     8761 tggcctcgat ccgtccatac ccggaagccc tgctgttcgg cagcctggac cgggccatcc
     8821 tcgcgatgct ccgggacggc gccgcccggt tcaccgcgcc gcagctcacc gcgtggcacg
     8881 aggccggcca cctctatcag gatctcccgt atctcacgcc cgccgtggtc cgcctggtgc
     8941 acgccaagat gcaggacctc tgcaaccagg tgcccggtgt agaatatggc tgtgttgtcc
     9001 atggtgatat ctccaccatg gacaagtgga ttccctacgc accggatgcc ctggactggt
     9061 acgggatcga cgtgtactgg aacgacccgt tcgacttcag catctatgac cagctgaagg
     9121 cgtacctcga cggttaccgg acgctggctc agcatcgcac cgggctgctg tacccgagga
     9181 tcaacgtctg cgcggcgaac agccacgacg agtcccgccg gcccggcttg ttcaagaacg
     9241 tcgccaggtg gctccaccac cacgggggca gccggatgct cacgtcctac caggacggcg
     9301 atccttcggg gggcccgtgg gccccgggcg acaccgacac gatcaacgcc ctcaagtaca
     9361 tcgcggccag ctacgtccat ccgcaggcgg gctccgagcg gaggcgtgcc tcgttctggg
     9421 aacccaagcc caggctgtcc tgagccgcag gcgatcgcag gcgcccggac ccgctcgatc
     9481 tgcagcacac ctccgcgcgt tctgttagct tcgccgcgtg aacaagctca tcgacgacat
     9541 cacgcggctg gagcggccga ccccgtcgga ctcccgggtc gcggagttcg gcagcgacgt
     9601 cgtcgccgag accctccgcg cgctcgagat tccctacatc gccgtcaacc cgggcgccag
     9661 ctatcgcggc ctgcacgact cgatcgtcaa tctcctcggc aacgagcggc cgcagatgct
     9721 gctgtgcctg cacgaggaga gcgcggtcgc gatcgcccac ggctacgcga aggtcaccgg
     9781 gcgggcgatg gcggtcgccg tccactccaa cgtcggcctg cttcacgcca ccatggcgat
     9841 cttcaacgcg tggtgcgacc gcatgccggt cctcgtcctc ggcgcgaccg gccccgtcga
     9901 tgcgaccagg cgccggccct ggatcgactg gatccatacc gccgccgatc aaggtgcggt
     9961 cgtccgccac tacaccaagt gggacgatca gccggcctcg gcgggcgccg cgcgcgaatc
    10021 gctgctgcgc gccgtctggc tcgcgaacag cccgccgcag gctcccgtct acgtcaacct
    10081 cgacgcggag atgcaggaag ccaggctgcc ggctccgctg ccgccgatcg atgcgcggcg
    10141 gttcttgccg cgcgtggtcg caggtccgcc ggcggacgtc gtgccccggg cggtcgagcg
    10201 actcaccgcg gccaggcgcc cgctgatcct ggccggccgc gtctcgcgcg acctcgccgc
    10261 gtggaaccgc cgcgtggagc tcgccgaggc gctcggcgcc cgtgtcgcga ccgctctcaa
    10321 ggtcggtgcg gcgtttccga ccgaccatcc gctgcacgcc ggcgcacgga ccacgctcgc
    10381 gaccgacgat gtcccggccg ccagtcgc
//
LOCUS       DASVOR010001701         9293 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_485163,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001701 DASVOR010000000
VERSION     DASVOR010001701.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9293
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485163"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..445)
                     /locus_tag="VF516_31785"
     CDS             complement(194..445)
                     /locus_tag="VF516_31785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112370.1"
                     /translation="MLQKRTFKVLAAIPKRDGGHWWMRCGAGHTNKDDSINVYLDAVP
                     RDLKFTLRELDEEDLRRREAYRATHGESAAPPPGDAPPF"
     gene            643..803
                     /locus_tag="VF516_31790"
                     /pseudo
     CDS             643..803
                     /locus_tag="VF516_31790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011813231.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1494..4460
                     /locus_tag="VF516_31795"
     CDS             1494..4460
                     /locus_tag="VF516_31795"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX8112371.1"
                     /translation="MQLAIVVDNKDGEGNPGYRVKVKFPWLNEQETSFWARIAVPMGG
                     ADRGTYVLPEIDDQVLVVFEHGDINRPIVVGSLWSRKQEPVEVNQSGKNHTKLIKSRS
                     GHRIIFDDKEGAEKIVIVDKTKKNKIVLDSVNKIVKIESDGDVEVKAKANLIIHSNAL
                     KVGTKEGITGKAQSLLTHSAKTFGLKATSVSGSGAGELGGAAAEPAPDPIAERGRGTG
                     GGGAGGAARGGGGGARGGGAGSGGGGPSGASPAAPNPVAPTPIDATTIEIEIIDVGGT
                     PRAGVAWDLSLPDGSVHSGTTGADGLIRRTGITTPGTAHLVLPDFDDEHGRAGGAQTP
                     GAVPYAAGGVDVQVGTRTRVEVEPRIYRGRLTGAHFDTDKTFMRPEAMNGIRLLVRFY
                     QEHQGANVVVSGHADTVGDPTYNVCLSNERARAVAAFLIDDVSVWLGFYRGSPCSKTW
                     GTTEDRMMLSTLTDASGQPFYQAADGNTNTPAMQRYQRARSLARTDGVPDEATRRSLI
                     GDYMALDGTSLPAGTHVESHGCGENHPRDDLGNNRDDLENRRVEIFLFEDAIRPAVPT
                     SCPPPRGCDAYPAWVAATVQTVDFRHAAGSFDGRVIVRSGGTDTPVTGATVHLSGPTS
                     ASGTTAADGTVRIDNLAAGHYSVTAHKDGSGDALVSVDIPEGGANPRLDIRLAAVTVD
                     WVDLTARCGDAVRIAGDFSGDGAPGAAVIEILNAADNAIVDTIQATFAGNHVDASWDC
                     RAVSAAWRTDRYLARLRLTDRGAAATSPRRFQLRDRPTTGFVLRSTSRGTPAGFAGIC
                     EKVDAALEASRVHYSLKLRLTGAAFSAAKQADAKSHIEAAWNNGFTGKAFHRHGCRRG
                     ATCNCGFDCCKVGYRLDVNFVASGEHYAVEIKIATDPANPERSGTSCSGSDWVDPPIS
                     VATSYAHEVGHMLGQFDEYPTGGTDPSGVQPANAPTANLMKTSGVTTLFNRHYRWVLE
                     FLNASTGGDLYETIPP"
     gene            4441..5337
                     /locus_tag="VF516_31800"
     CDS             4441..5337
                     /locus_tag="VF516_31800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112372.1"
                     /translation="MRPFHRRPASACALALAVVLAGCTLEHHMKFDRIEYQYAASMAG
                     ANTLTVHADGRARYESHTSGAEPQIGRYETQLAPAEIEALDRAIADPPLSGLRDHAGR
                     VRAGDRYTRVRMTLGATTIDKMVGTREPVDAGLGRVIDSLKAIIDKVMRHPREVLRMD
                     ASALAVDRHATLTATLRFSNPGAQPIAWLPPSGHLTLRAWPDKPVADFVPGDLFELRG
                     AQLSCVAATPGAPAALAHLAPGGEAVYQVSATLAPRAPGIHAIQLIYENTDEQVDAQP
                     VLVGQISAPTVKLELAATAKPR"
     gene            5709..5864
                     /locus_tag="VF516_31805"
     CDS             5709..5864
                     /locus_tag="VF516_31805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112373.1"
                     /translation="MQKKTKQLKTLRLRKEALRVLTKEQQLRVHAGDAADPWDPPLTF
                     TCPTVEA"
     gene            5993..7120
                     /locus_tag="VF516_31810"
     CDS             5993..7120
                     /locus_tag="VF516_31810"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX8112374.1"
                     /translation="MAAHLRVPRVAAPVPEFRSSYVATRQPVLIEGLFDGQPIRKLAT
                     ARAARRAIGDMDFVVQWNYIANLYYANAREAGQPERTTLANYLDYVALNPRTPRLCLE
                     QPIAPALAALIEAPALCWNNERTLMFIGNAGNFSHLHFDGDQRQVLMYQVFGAKRVIL
                     IPPIAAKKLLPHQKFSSVCVQHMSERERRDFLRYVGGCECLVRPGQAIFMPALFWHYV
                     EYVETSLSVHFRFGQTRYGRFFDDFVHRSMYPQNIAACFQDERFAAGPGHKVMRRLRR
                     AVAATYRNDRERYLHIEKLCKELYQQLCPDGFTGIHAVHGWAPSEADKCTAFYADRRR
                     APRRPPATAVSSRPDAGLGTSTRGTPRSQRGRAPGPARRRR"
     gene            7520..7690
                     /locus_tag="VF516_31815"
     CDS             7520..7690
                     /locus_tag="VF516_31815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112375.1"
                     /translation="MDELVTVSRGSGTEIIELVRRLIVAPPAAARWAEPRVESFEGRG
                     MDDAVVIEHERA"
     gene            7876..8811
                     /locus_tag="VF516_31820"
     CDS             7876..8811
                     /locus_tag="VF516_31820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8112376.1"
                     /translation="MIPYARGLHELGDECYAYLQPDGSWGWSNAGLVRGAGASLLVDT
                     LFDLRLTAAMLEAMAGVRAGAPITTVVNTHANGDHCYGNQLVAGAEIVASAATAREMG
                     EVSPAMLAGLNAAPGAVGDMFRGFLGAFDFTGIELALPTRTFDGRLELDVGGRRVTLI
                     EVGPAHTRGDTLVHVPDARVVYTGDILFIGGTPVVWAGPLSNWIAACDLMLGMDVDIV
                     VPGHGPVTDKRGVAAVRDYLAYVDREATARFRTGMTAWDAARDIALGEFAGLGERGRI
                     AVNVEAVYRALDPAHRSPTIVELFQRMAALEHAGA"
     gene            8903..>9293
                     /locus_tag="VF516_31825"
     CDS             8903..>9293
                     /locus_tag="VF516_31825"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8112377.1"
                     /translation="MKAGELCIRNVATTTANETVVEAARRMAESRAGDLIIVEHRPCG
                     PARPIGIITDRDLVIRVLACPERLPATTTIAEVMRSGLVTASEDEEVERIVALMRDHA
                     IRRIPIVDRCGGLLGILSLDDVLGWMRE"
BASE COUNT         1504 a         3216 c         3162 g         1411 t
ORIGIN      
        1 gtcggcgccg ccgcgaccgg cttggtgacc gccgcggccg gcgggggcgc ggctgccgcc
       61 gagcgggtcc aggtgccgcc catcgcggcg acgagcagtg caacggccgc gagcgacgcg
      121 agccggtggg ggagcttgga gatcaactgg gtcatgacac ggtcctttcg tcagcagaca
      181 gaagagcggt ggatcagaac ggcggcgcgt cgcccggcgg cggggccgcc gactcgccgt
      241 gcgtcgcgcg gtatgcctcg cgccggcgca ggtcttcctc gtcgagctcg cgcagcgtga
      301 acttcagatc gcgcgggacc gcgtcgaggt acacgttgat cgagtcatcc ttgttggtat
      361 gccccgcgcc acaccgcatc caccagtggc caccgtcccg cttgggaatc gcggccagta
      421 ccttgaatgt tcgcttctgc aacacgacag ttctccttcc gacgccgatg cgtcgcgccg
      481 gcaaagagca actgcggtgc cacggctaac tacgcacgat cacacaatcg tctgtccggc
      541 ttttggccgg atctcggacg atcgtccgaa atccggccga aacccggaca cgcgacacgc
      601 gacacgcgac acgcgacacg cgagacctcc cgggcgctgg gcatctcgcc gcacgtagcg
      661 cagaaggccc agtacacggc gatcgacgcg cgaaccaccc gacaccctgg tacacggtga
      721 gccagcgcat tcgcaaacgt gtcgaagcga tcttcggctg ggcaaagacg gtcggtggct
      781 tccgtcgctc gcgattccgc ggaatcgcga gaaccccgca ggccgcgttc tttgtgggcg
      841 ccgcctacaa cctgctcagg atggcaaacc tggccatggc atggcgggcg tgcacggatg
      901 gcgctcgccg gcgccgccgc tgcaccgagc gatccggtgg ccgcttcgat gcaggaagcg
      961 aggagccagt tcgtcgggat ccctcccctt ttcaacatcc tgctaggcgc gtcgcgtctc
     1021 cagtccaggc gcccccaacc tggatcgacc ggggcgggct gatcgacttc acggtagagc
     1081 aggaacggcc acccgcggtc gtcggccgcc cctcggctgt agttgcgcct cgactgtgcg
     1141 ccactcccga ggttctgctt cttacagcaa tcttgcagtt atgtcgtctg taaataacgc
     1201 tcggaatagt cttctcagat gcacgcgaca attgctgccc catgacgaat tcgagtaggc
     1261 tccacagata tatggggcgc gcgtggggta ggagccgtgc tggttgcatg cgattgatga
     1321 agctgtatgc gtcatcgctg aaccagccgc accgcaggac cagtgctgcg aaatgggtct
     1381 tcagcgactg caggaactcg acgcaccttt gagcgacatc gaggtaacct gtgggctttc
     1441 tacccatcga tcacatgagg ccaccaggtc ttcgcacctc gaagatccgg ggtgtgcagc
     1501 tggcgatcgt cgtcgacaac aaggatggcg aaggcaatcc cggctaccgg gtcaaggtca
     1561 agtttccctg gctcaacgag caggagacat cgttctgggc tcggatcgcg gttccgatgg
     1621 gaggggccga ccgaggtacc tacgtgctcc ccgagatcga tgatcaggtg ctcgtggtgt
     1681 tcgagcacgg cgatatcaac cggccgatcg tcgtcggttc attgtggagc aggaagcagg
     1741 agccggtcga ggtcaaccag tccggcaaga accacaccaa gctcatcaag tcgcgctctg
     1801 gccaccggat catcttcgac gacaaggaag gcgccgagaa gatcgtcatc gtcgacaaga
     1861 cgaagaagaa caagatcgtt ctcgactcgg tcaacaagat cgtgaagatc gagtccgacg
     1921 gtgatgtcga ggtcaaagcg aaggccaacc tgatcatcca ctcgaacgcg ctcaaggtcg
     1981 gcacgaagga aggcatcacc ggcaaggccc agtcgctgct cactcactct gccaagacgt
     2041 ttggtctcaa ggcgaccagc gtgtcgggct ccggtgccgg cgagctggga ggggctgcgg
     2101 cggagccggc gccggatccg atcgcggagc ggggccgtgg caccggcgga gggggcgcag
     2161 gcggagcggc tcgtggtggt gggggaggcg cacgtggcgg cggcgctggc tcgggtggcg
     2221 gtggcccgag cggcgcctcg ccggccgcgc ccaatccggt ggcaccgacg ccgatcgatg
     2281 ccacgacgat cgagatcgag atcatcgatg tcggcggcac gccgcgcgcc ggcgtcgcct
     2341 gggatctgtc gctgcccgat ggctcggtgc acagcggcac gaccggcgcc gacggtctga
     2401 tccgccgcac cggcatcacc acaccgggga cggcgcacct cgtgctgccc gacttcgacg
     2461 acgagcacgg acgcgcgggc ggcgcgcaga caccgggcgc ggtgccctac gccgcgggcg
     2521 gggtcgacgt tcaggtcggc acgcgcaccc gcgtcgaggt cgagccgcgc atctaccgcg
     2581 gtcggctgac cggcgcgcac ttcgacaccg acaagacgtt catgcgcccc gaggcgatga
     2641 acggcatccg gctgctggtg cggttctacc aggagcacca gggggcgaac gtcgtggtga
     2701 gcggccacgc cgacacggtc ggcgatccga cctacaacgt ctgcctgtcc aacgagcgcg
     2761 cccgcgcggt ggccgcgttc ctgatcgacg acgtcagcgt atggctcggg ttctaccgtg
     2821 gctcgccctg ctcgaagacc tggggcacca ccgaggaccg aatgatgctg agcacgctga
     2881 ccgacgcctc cggtcagccg ttctaccagg ccgccgacgg caacaccaac acgccggcga
     2941 tgcagcgcta ccagcgcgcg cgctcgctgg cgcgcaccga cggtgtcccc gacgaggcca
     3001 cgcggcgctc gctgatcggc gattacatgg cgctcgacgg cacctcgctg cccgcgggta
     3061 cccacgtcga gtcgcacggc tgcggcgaga accatccgcg cgacgacctc ggcaacaacc
     3121 gggacgacct ggagaaccga cgcgtcgaga tctttctgtt cgaggatgcg atcaggccgg
     3181 ccgtaccgac gagctgcccg ccgccgcgcg gctgcgacgc gtatcccgcg tgggtcgccg
     3241 ccaccgtgca gaccgtcgac ttccggcacg cggcgggcag cttcgacgga cgggtcatcg
     3301 tgcgcagcgg cggcaccgac acgccggtca ccggtgccac cgtccacctc tccgggccga
     3361 cctcggcgag cggcaccacg gccgccgacg gcacggtgcg catcgacaac ctcgcagccg
     3421 ggcactacag cgtgaccgcg cacaaggacg gatccggcga tgcgctggtg tcggtcgaca
     3481 tccccgaggg cggtgcgaac ccgcggctcg acatccggct cgccgcggtc accgtcgact
     3541 gggtcgatct caccgcgcgc tgtggagacg cggtgcgcat cgccggcgac ttcagcggcg
     3601 acggcgcgcc cggcgcggcc gtgatcgaga tcctcaacgc cgccgacaat gcgatcgtcg
     3661 acaccatcca ggctacgttc gccggcaacc acgtcgacgc cagctgggac tgtcgcgcgg
     3721 tcagcgccgc ctggcgcacc gatcgctacc tcgcgcgcct gcgcctcacc gaccgcggcg
     3781 cggcggcgac ctcgccgcgg cggttccagc tgcgggaccg cccgaccacc ggcttcgttc
     3841 tgcgcagcac cagccgcggt acccccgccg ggttcgcggg tatctgcgag aaggtcgacg
     3901 ccgccctcga ggccagccgc gtccactact cgctcaagct ccggctgacc ggcgccgcgt
     3961 tctcggcggc gaagcaggca gacgcgaaat cgcacatcga ggccgcctgg aacaatggct
     4021 tcacgggcaa ggcgttccat cgccatggct gtcggcgcgg ggcgacatgc aactgcggat
     4081 ttgattgctg caaggtcgga tatcggctcg atgtgaactt cgtcgccagt ggggagcact
     4141 acgcggtcga gatcaagatc gcgaccgacc cggccaaccc cgagcgcagc ggcacgagct
     4201 gcagcggcag cgactgggtg gatcctccga tctcggtcgc cacgtcgtac gcgcacgagg
     4261 tcggccacat gctcggccag ttcgacgagt acccgaccgg cggcaccgat cccagcggcg
     4321 tgcagcccgc caatgcgccg accgccaact tgatgaagac ttccggcgtc acgacactct
     4381 tcaatcgcca ctaccgctgg gtgctagagt tcctgaatgc cagcaccggc ggtgacctct
     4441 atgagaccat tccaccgtag gccagcctcg gcgtgcgcgc tcgcgctcgc ggtcgtgctc
     4501 gccggctgta ccctggagca ccacatgaag ttcgaccgga tcgagtacca gtacgcggcg
     4561 tcgatggccg gggccaacac gctcaccgtg cacgccgacg ggcgagcccg ctatgaatcc
     4621 cacacctccg gggccgaacc ccagatcggc cgctacgaga cccagctggc gcccgccgag
     4681 atcgaggcgc tcgaccgcgc gatcgcggat ccgcccctga gcggcctgcg tgatcacgcg
     4741 ggccgcgtcc gcgccggcga tcgctacacg cgcgtgcgca tgacgctggg ggcgaccacg
     4801 atcgacaaga tggtcggcac ccgggagccg gtcgatgccg ggctcggccg ggtgatcgac
     4861 agcctgaagg caatcatcga caaggtcatg cgccatccgc gcgaggtcct gcgcatggac
     4921 gccagcgcgc tcgccgtcga ccgccatgcc accctcactg caacgctgcg gttctcgaat
     4981 ccgggcgcgc agccgatcgc gtggctgccg ccgtccggtc atctgacgct gcgcgcgtgg
     5041 cccgacaagc cagtcgcgga cttcgtcccc ggcgacctgt tcgagctccg gggtgcgcag
     5101 ctatcgtgcg tcgccgcgac gcccggtgcg ccggccgcgc tcgcgcatct cgcgcccggc
     5161 ggcgaggccg tgtaccaggt ctcggcgacg ctcgcgccgc gcgcccccgg catccatgcg
     5221 atccagctga tctacgaaaa caccgacgag caggtcgatg cgcagccagt gctggtcggc
     5281 cagatctccg cccccacggt caagctcgag ctcgccgcga cggcgaagcc gcgctgagcg
     5341 cgcgatagca tccttgccca cgacctcgtg gcgaggcgct accccaacgg cgctcctacc
     5401 tcgatcgggt ccctaccccg gtcccagagc ctgcagccgg ctgacctcaa gggcgacgcc
     5461 ggcgctcgaa gcgcgggccg gtaactcgtc ggtaaccgtc aggaatggac gatcgccgat
     5521 cgtgctgatc gaggatgctc tgcttcgtca cgtcgattac tacggcgggg cagtgcgacc
     5581 cgactcccgc catgttttcg accggatgcg cacgatagcc ggcgcggggc taccgaccgg
     5641 ccatggtaga caatgatcct ccatcagagc gactttactt caatacgaaa aatcactacc
     5701 gtctcgccat gcaaaagaag actaagcagc tgaagaccct ccgtcttcgc aaggaagccc
     5761 tccgggtcct cacgaaggag cagcagcttc gtgtacacgc cggcgatgcc gccgacccct
     5821 gggatcctcc gctgaccttc acctgcccca ccgtagaagc atagaagctt cctgatgccg
     5881 gtcaggcgct agcccccggg gctggcacca ggccttgcgt gcgctgcagg gctggccggt
     5941 ttcatcgtcg ggatagagcc gcacgacgac tgggtcatct cgaggaggat ccgtggctgc
     6001 ccatctccgc gtcccgcgag tcgccgcccc ggtaccggag ttccggtcgt cctatgtcgc
     6061 gacccgccag ccggtgctca tcgagggtct attcgatggc cagcccatcc gcaagctcgc
     6121 gaccgcgcgc gccgcgcggc gcgcgatcgg cgacatggac ttcgtggtgc agtggaacta
     6181 catcgcgaat ctctactacg caaacgcccg agaagcgggc cagccggagc gcacgacgct
     6241 tgccaactac ctcgactacg tggcgttgaa cccgcgcacc ccgcggctgt gcctcgagca
     6301 gccgattgca cccgcgctcg ccgccctgat cgaggcgccg gccctgtgct ggaacaacga
     6361 gcgcacgctg atgttcatcg gcaatgccgg taacttctct catctccact tcgacggcga
     6421 tcagcgccag gtgctcatgt atcaggtatt cggcgccaag cgcgtcatcc tgatcccgcc
     6481 gatcgcggcg aagaagctgt tgcctcacca gaaattcagc tcggtctgtg tccagcacat
     6541 gtcggagcgc gagcgccgtg acttcctgcg ctatgtcggc ggctgcgagt gtctggttcg
     6601 gccgggtcag gcgatcttca tgccggcgct gttctggcac tacgtcgaat acgtcgagac
     6661 gtcgctctcc gtccacttcc gcttcggaca gaccaggtac ggtcgcttct tcgacgactt
     6721 cgtgcatcgc agtatgtatc cccagaacat cgccgcgtgc ttccaggacg agcggttcgc
     6781 cgcgggaccc ggccacaagg tcatgcgccg acttcgccgc gccgtggccg cgacataccg
     6841 caacgaccgc gagcgatact tgcacatcga gaagctgtgc aaggagctct accagcagct
     6901 gtgccccgac ggcttcaccg gcattcacgc agtccacggt tgggcaccgt ccgaggcgga
     6961 caagtgcacg gccttctatg ccgaccggcg gcgtgcgccg cgccggccgc cggccaccgc
     7021 cgtgtccagc cgcccagacg cgggcctcgg tacatcgacg cgaggtactc cgcgatcgca
     7081 gcgcggtcgg gctccgggac cggcgcgccg tcgacggtga ccaccttggc ggtacatcgc
     7141 ccgtggtcaa gccaccgccg gcgtcgatcc gcccgagacc gcgccggcca ccgtgctctc
     7201 gcgtggcagc gtcgagagtc gttcgcgcgt ccaggtcgcg cacgctgtgc ccctgggtcc
     7261 atgcgtcggt gtagacgctg tgcgcacgtg tggatcagga ggggcccgcg cggcgccggc
     7321 tagtcatgca tcacggctgg tgaagacgcc cgcacgcgtg accacgagat cgggctcggc
     7381 ccatcgcgac cggtggcccg cgatttgaac tcgctgtccg tcggagaggc ggttgtgtac
     7441 gagaggcgac accatcacac gcgctgactt cgcggtggag cgcgggtcgg cgtgccgcgc
     7501 cgaggcgaac gaaagcggcg tggacgagct cgtcaccgtg tcgcgcggtt ccggcacgga
     7561 gatcatcgag ctggtgcgcc gcctgatcgt ggcgccgccc gctgctgccc ggtgggcgga
     7621 gccccgcgtc gaatcgttcg agggacgcgg catggacgat gccgtcgtga tcgagcacga
     7681 gcgggcgtag cctccgcgac gaggtgcgcc gcttgctgtg cgtgcggctc cgggtgtcga
     7741 ccgaggagct caccagcttg atccggctcg ttcgcagcaa gctccagatc agcgtcacgt
     7801 cgctgctggc gtcgtagcca gtcacgcgtg aatcgcggtg gctcgcgagc ctgggagtag
     7861 cgataggatg cgcgggtgat tccctacgca cgcgggctcc acgagctcgg agacgagtgt
     7921 tacgcgtacc tgcaacccga cggcagctgg ggctggagca acgcggggct ggtgcgcggc
     7981 gccggcgcgt cgctgctggt cgacacgctg ttcgatctgc ggctgaccgc cgcgatgctg
     8041 gaggcgatgg ccggggtccg cgccggcgcg ccgatcacga ccgtggtgaa cacccacgcc
     8101 aacggcgatc actgctacgg aaaccagctc gtggccggcg ccgagatcgt cgcgtcggcg
     8161 gcgaccgccc gcgagatggg cgaggtgagc ccggcgatgc tggccgggct gaacgccgcg
     8221 cccggcgcgg tcggcgacat gttccgcggg ttcttggggg cgttcgactt caccggcatc
     8281 gagctcgcgc tgccgacgcg cacgttcgac ggccggctcg agctcgacgt cggcgggcgg
     8341 cgcgtgacgc tgatcgaggt cgggccggcc cacacgcgcg gcgacacgct ggtgcacgtg
     8401 ccggacgcgc gcgtcgtgta caccggcgac atcctgttca tcggtgggac gccggtggtg
     8461 tgggcggggc cgctgtcgaa ctggatcgcg gcctgtgatc tcatgctcgg catggacgtg
     8521 gacatcgtgg ttccgggcca cgggccggtg accgacaagc gcggggtggc cgcggtgcgc
     8581 gactacctgg cgtacgtcga ccgcgaggcg acggcgcgct tccgcaccgg gatgaccgcg
     8641 tgggacgctg cgcgcgatat cgcgctgggc gagttcgccg ggctcggcga gcgcgggcgg
     8701 atcgcggtca acgtcgaggc cgtgtaccgg gcgctcgatc cggcgcaccg ctcgccgacc
     8761 atcgtcgagc tgttccagcg gatggcggcc ctggagcacg cgggcgcctg accggcgtcg
     8821 tcgagccgct gcgccttcgc ctgcggcgga ttcacccgcg cgcgaaccgc ggcctggaac
     8881 gcgggatgca ggctcaacgg gcatgaaggc cggggagctg tgcatccgca acgtggccac
     8941 cacgacggcc aacgagacgg tggtggaggc ggctcggagg atggcggagt ctcgcgccgg
     9001 cgatctgatc atcgtcgagc accggccgtg cgggccggcc cggccgatcg ggatcatcac
     9061 cgaccgcgat ctggtgatcc gggtgctcgc gtgcccggag cgcttgccgg cgaccacgac
     9121 gatcgccgag gtcatgcgat ccggcctggt gacggcgagc gaggacgagg aggtcgagcg
     9181 gatcgtcgcg ctgatgcgcg atcacgcgat tcgccggatc ccgatcgtcg accgctgcgg
     9241 cgggctgctc gggatcctca gcctggacga cgtgctgggc tggatgcgcg agc
//
LOCUS       DASVOR010001702         2175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_485178,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001702 DASVOR010000000
VERSION     DASVOR010001702.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2175
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485178"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..438)
                     /locus_tag="VF516_31830"
     CDS             complement(<1..438)
                     /locus_tag="VF516_31830"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX8112378.1"
                     /translation="MTEPAALPPAWENPAFVAVRCFACGEPHDRHTALTVCRSCGLPL
                     RVDYDHARAPLAPSALLGRPASLWRYREVLPIRPEHAVSLVEGWTPLCPIGDRLFVKD
                     ESRNPTGSFKARGIALAVSMARALGARALSAPSAGNAAGALAAY"
     gene            complement(549..1040)
                     /gene="pcaG"
                     /locus_tag="VF516_31835"
     CDS             complement(549..1040)
                     /gene="pcaG"
                     /locus_tag="VF516_31835"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HEX8112379.1"
                     /translation="MPPVTASQTIGPFWHILGESGLDDLTRLGATGPRIELTGRVIDG
                     DGAPVTDGCVEIWQATPPASPDFLGWGRCATDPHGNFRFVTLAPDPAAPVLSVAVFAR
                     GLLKPLWTRVYLVEPEAKIDDPLLAALPPERRATLLARLEDGRWRWDIRLQGDGETVF
                     LDL"
     gene            complement(1040..1750)
                     /gene="pcaH"
                     /locus_tag="VF516_31840"
     CDS             complement(1040..1750)
                     /gene="pcaH"
                     /locus_tag="VF516_31840"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HEX8112380.1"
                     /translation="MTRAAYAPFPPGTQPAYDVPSYTSTHRRAPHQPLIALPAAPPPM
                     EHAGPVFTPHQFPVAIDLSRENGRDAMGQRIIVAGRVTDDADRPVAGAMIELWQANAA
                     GRYHHERDAHDAPLDPNFRGAGRVFTDGDGRYRFVSIKPGAYPWRNHPNAWRPNHIHF
                     SLFGPTWGARLVTQMYFPGDPLLAIDPIYQSVPDPDARARLVAAFDLSLTQPEWALGF
                     RFDIVLRGLRATPMEGAR"
     gene            complement(1747..>2175)
                     /gene="pcaC"
                     /locus_tag="VF516_31845"
     CDS             complement(1747..>2175)
                     /gene="pcaC"
                     /locus_tag="VF516_31845"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: protein motif:HMM:TIGR02425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HEX8112381.1"
                     /translation="MLDHPDDVTAAMRDFLTPRDADLATAGARVRSEVLGADHVARAT
                     AAATDLDRDFQAYLTRAAWGGVWARPHFDRRTRSIVTVALLAALGREHELALHIRAMR
                     NTGASRADLTELLLHVAVYAGVPAANAAMRIAKQVAEETP"
BASE COUNT          295 a          670 c          943 g          267 t
ORIGIN      
        1 gtaggcggcg agggcgccgg cggcgttgcc ggcagacggg gcggagaggg cgcgagcgcc
       61 gagcgcgcgg gccatggaga ccgcgagggc gatgccgcga gccttgaacg agccggtggg
      121 gttgcgcgac tcgtccttga cgaacaggcg atcgccgatc gggcacagcg gggtccagcc
      181 ctcgaccagg ctgaccgcgt gctcggggcg gatcggcagg acctcgcgat accgccacag
      241 ggaggccggc cggccgagca gcgcggacgg ggcgagcggc gcgcgggcgt gatcgtagtc
      301 gacgcgcagc ggcagaccgc acgagcggca gacggtcagc gcggtgtggc ggtcgtgcgg
      361 ctcgccgcac gcgaagcagc gaacggcgac gaacgccggg ttctcccacg cgggcggaag
      421 ggcggcgggc tcggtcatgt ggcgcgcagc atagccgagg cggccgcccg ctgcggccag
      481 caacacgctt gacgtcacgc caaccgggtt ggcagttatc gaaggagagg gcgtgcttcg
      541 atagcgcctt atagatccag gaacacggtc tcgccgtcgc cctgcaggcg gatgtcccag
      601 cgccagcggc cgtcctcgag gcgggcgagc agcgtggcgc ggcgctccgg gggcagcgcg
      661 gcgagcagcg ggtcgtcgat cttggcctcg ggctcgacga ggtagacccg ggtccacagc
      721 ggcttgagca ggccgcgggc gaacacggcg accgacagga cgggggccgc ggggtcgggt
      781 gcgagggtga cgaaccggaa gttgccgtgc ggatcggtgg cgcaccggcc ccagccgagg
      841 aagtcgggcg acgcgggcgg ggtggcctgc cagatctcga cgcagccgtc ggtgaccggg
      901 gcgccgtcgc cgtcgatcac gcggccggtg agctcgatgc gcgggccggt ggcgcccagc
      961 cgggtgagat cgtcgaggcc ggactcgccg aggatgtgcc agaacggtcc gatggtctgg
     1021 ctcgccgtga ccgggggcat caccgcgctc cttccatcgg ggtggcgcgc aggccgcgca
     1081 gcacgatgtc gaaccggaag ccgagcgccc actcgggctg ggtcagcgac aggtcgaacg
     1141 cggcgaccag gcgagcccgc gcgtcgggat cggggaccga ctggtagatc ggatcgatcg
     1201 cgagcagcgg atcgccgggg aagtacatct gggtgaccag gcgggcgccc caggtcgggc
     1261 cgaacagcga gaagtggatg tggttggggc gccaggcgtt ggggtggttg cgccaggggt
     1321 aggcgcccgg cttgatcgag acgaagcggt agcggccatc gccgtcggtg aacacccggc
     1381 cggcgccgcg gaagttgggg tcgagcgggg cgtcgtgggc gtcgcgctcg tggtggtagc
     1441 ggccggcggc gttggcctgc cacagctcga tcatcgcgcc ggcgaccggg cgatcggcgt
     1501 cgtcggtgac gcggccggcg acgatgatgc gctggcccat ggcgtcgcgg ccgttctcgc
     1561 gcgacaggtc gatcgcgacg gggaactggt ggggggtgaa caccgggccg gcatgctcca
     1621 tcggcggcgg tgcggcgggg agcgcgatga gcggctggtg cggggcgcgg cggtgggtgc
     1681 tggtgtagct cgggacgtcg tacgccggct gggtgccggg cggaaacggc gcgtacgcgg
     1741 cgcgggtcat ggcgtctcct cggcgacctg cttggcgatc cgcatcgcgg cgttggcggc
     1801 cgggacgccg gcgtagaccg cgacgtggag caggagctcg gtgagatctg cgcggctggc
     1861 gccggtgttg cgcatcgcgc ggatgtggag cgcgagctcg tgctcgcggc cgagcgcggc
     1921 gagcagcgcg acggtgacga tgctgcgggt ccggcgatcg aagtgcgggc gcgcccacac
     1981 gccgccccac gcggcgcggg tgaggtaggc ctggaagtcg cggtcgaggt cggtggcggc
     2041 ggcggtcgcg cgggcgacgt ggtcggcgcc gagcacctcg ctgcggacgc gtgcgccggc
     2101 ggtggcgagg tcggcgtcgc gcggggtcag gaagtcgcgc atcgcggcgg tgacatcgtc
     2161 ggggtgatcg agcat
//
LOCUS       DASVOR010001703         2278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_485179,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001703 DASVOR010000000
VERSION     DASVOR010001703.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485179"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..537
                     /locus_tag="VF516_31850"
     CDS             169..537
                     /locus_tag="VF516_31850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112382.1"
                     /translation="MRWPGVVQAANIIDEDIQVPPKAAAQVYHWARHVLDKLKRAITP
                     AHLHVLELLDGHKVARCLCVAVQLGIADQLNDGPRACTDLARAIDVNADALASEAREL
                     RCRALRQLGRGDECAPASRP"
     gene            complement(560..793)
                     /locus_tag="VF516_31855"
     CDS             complement(560..793)
                     /locus_tag="VF516_31855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112383.1"
                     /translation="MEQDSGLCPATITACGDITVVSQSRGDAATLWYHQGGQLVAIVN
                     QLSPGRYMCLAGPDVFVLPACTPSSQSLPACGS"
     gene            1190..>2278
                     /locus_tag="VF516_31860"
     CDS             1190..>2278
                     /locus_tag="VF516_31860"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112384.1"
                     /translation="MVDTTVTPSIPGGGGIDPDLQPGQIIGEYRVEAKLGQGGFGAVF
                     KAVHPLIGKLVAIKVLLQRFSVDPQMVSRFVAEARAVNQIRHHNIIDIFSFGTLDDGR
                     HYYVMEYLDGETLERRIARQGRLSLREALPILRAIARALDAAHAKGIAHRDLKAENVF
                     LASNPDGVFPKLLDFGIAKLLAPEDGLAHKTRTGAPLGTPHYMSPEQCHGRDVDHRTD
                     LYAFGVLAYVMLTGVFPLDGDDYMTILMRQLHDEPPPASSHRPDLPAGIDRVIAWLMR
                     KDPAERPTTLAEALRSLEHVASGELTAAEPSASTRLATAETPSSLPELPAFPSGNRTP
                     SAPGASARSGAPAGDLPRPAAAAAFQTRE"
BASE COUNT          348 a          792 c          813 g          325 t
ORIGIN      
        1 tgtgcatcgc cgggcccgac gtgttcgtcc ttccctcgtg cacgccgtcg agccagagcc
       61 tgccggcttg cggctcttga cggcagatca ggaaccggtg gggaacttct cggtcaagtc
      121 gctcggaagc atggccgggc gggatcgtga gggtatggtc cgcgtgtcgt gaggtggcct
      181 ggagtcgtgc aggcagcgaa catcatcgat gaggacatcc aggtgccgcc gaaggccgcg
      241 gcgcaggtct atcactgggc gcgtcacgtc ctcgacaagc tgaagcgagc gatcacgccc
      301 gcccacctgc acgtgctcga gctgctcgac ggccacaagg tcgcacggtg cctgtgcgtc
      361 gcggtgcagc tcggcatcgc cgaccagctg aacgacggcc cccgggcgtg caccgacctg
      421 gcccgggcga tcgatgtcaa cgcggacgcg ctggcctccg aggcgcgcga gctgcggtgc
      481 cgtgcgctgc gccagctcgg ccgcggcgat gaatgcgcgc ccgcgtcccg gccgtgaccg
      541 gcgaggcgcg agcctgccgt caagagccgc aagccggcag gctctggctc gacggcgtgc
      601 acgcgggaag gacgaacacg tcgggcccgg cgaggcacat gtaccgcccc ggagacagct
      661 ggttgacgat cgcgacgagc tgcccgccct ggtggtacca gagcgtggcg gcgtcgcccc
      721 ggctctggga caccacggtg atgtcaccgc acgcggtgat cgtcgccggg cacaggccgc
      781 tgtcctgctc catggcatcc agggttcgac cgcaggggtt tgcggtgcag tagtccgcga
      841 cgttggtccg gcaggccacg cggacggggg gctcgggagg tggggtcgaa ggaggggggc
      901 tgtgcgtgca ggagaagcac gccagcgaca cgacacatgc caggatccgc atgacccatt
      961 gtctcgcaac gaccgcgcca ggcagaagct ctgaagttcc acggcgagca ttccaccgaa
     1021 gccgagcgcc cacggccaat cgacatcccc ggtacgagcc cggacgtcgt gccgccgtca
     1081 caggcctgta tcgcacaggt acacatggtg cgacgggtgc tgccgatgtc ggcgctggac
     1141 ctgcgggcgt cattgacctg cgggcggttc gcttcgacaa tgaggcctgg tggtcgatac
     1201 cacggtgaca ccctcgatcc cgggaggcgg cgggatcgat ccggatctcc agcccggcca
     1261 gatcatcggc gagtaccggg tcgaggccaa gctgggtcag ggtggcttcg gcgcggtgtt
     1321 caaggcggtg cacccgctga tcggcaagct ggtcgcgatc aaggtgctgt tgcagcggtt
     1381 ctcggtcgat ccgcagatgg tgtcgcggtt cgtggccgag gcgcgcgcgg tcaaccagat
     1441 ccgccaccac aacatcatcg acatcttctc gttcggcacg ctcgacgacg gccgccacta
     1501 ctacgtgatg gagtacctgg acggcgagac cctcgagcgc cggatcgcgc gccagggtcg
     1561 gctgtcgctc cgcgaggcgc tcccgatcct gcgtgcgatc gcccgcgcgc tcgacgccgc
     1621 gcacgccaag gggatcgcgc accgcgacct caaggccgag aacgtgttcc tcgcgtcgaa
     1681 ccccgacggt gtgtttccca agctgctcga cttcgggatc gcgaagctgc tcgctccgga
     1741 ggacgggctc gcgcacaaga cgcggaccgg cgcgccgctg ggaacgccgc actacatgtc
     1801 gccggagcag tgccacggcc gcgacgtcga tcaccggacg gatctctacg cgttcggcgt
     1861 gctggcgtac gtgatgctca ccggggtgtt cccgctcgac ggcgatgact acatgacgat
     1921 cctgatgcgg cagctccacg acgagccgcc gccggcgtcg tcgcaccggc cggacctgcc
     1981 cgcgggcatc gaccgcgtga tcgcctggct catgcgcaag gatccggcgg agcggccgac
     2041 gacgctcgcc gaggccctgc ggagcctcga gcacgtcgcg agcggcgagc tgaccgcggc
     2101 cgagccatcc gcgagcacgc ggctcgccac ggccgagacc ccgagctcgc tgcccgagct
     2161 gccggcgttc ccgtccggga accggacacc ctccgcgccg ggggcgagcg cgcggtccgg
     2221 ggccccggcc ggcgatctgc cccgcccggc ggccgcagcc gcgttccaga cccgcgag
//
LOCUS       DASVOR010001704         2116 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_251583,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001704 DASVOR010000000
VERSION     DASVOR010001704.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251583"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2116)
                     /locus_tag="VF516_31865"
     CDS             complement(<1..>2116)
                     /locus_tag="VF516_31865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112385.1"
                     /translation="PETLRELAELVRGKIGALPARCRAGVSVRVCLSHMDQIDGYDEL
                     VAVVGAQHGPLDVRALGDRLTDARAVSAAARALIAKFDGNLAYGLVHRTSDGFARLVG
                     FYTTFPVLLAQLAPLLHALTGDDADQPHYPPSDLYLTSLAPDNHVGDPFTVDHDLVAA
                     SIAQQRRFHRRTGLAAAAAGVAVVGALMWRHQGQVAAAGQAVDGYDAQVTLGRGPGEI
                     QARRVAATIGRMHDGERLWLDHTFIERKRQVEDTFADHLREQYILPKLRVPTINRSTM
                     LYIVGLLYASEANGLRALIRDNLALWVSKLDLSMTVVSTYLDVSRYQYEIAETFDPVY
                     TGSDWQSYAFGRIKPLYDQVQPLSQAQLDDLDRDPPQLYDPREYAVRRSIVELLSAQL
                     ALATQPSIKKLLDSPLGTSEWVEANVDALGGINAAVAHHQLAPASPRTLAELGADLER
                     MLAAPATGREVYRVSRVQGGSPEVFDFDVAVWNHKLAEASAALMIALVHAQSLAHPER
                     AIGFFPAGAAPGDGTESGAQGPTVRLPATYTAAAFAQQVAPALDFITTRAGNLGLPSE
                     ELAQLTELYRAQIDDYAARYAGALRAYYGSFRFDPGSEEALPFALTAMVQPSSWFLRF
                     LTTVSTNAAPVLGDGPYYQVMAESLADFRALAELLAPAKGTIPGLAWYQQLITQLAAV
                     LDPAAGATAGGAASAPAASGGGD"
BASE COUNT          290 a          723 c          816 g          287 t
ORIGIN      
        1 atcgccgccc ccgctcgcgg ccggcgcgct cgcggccccg cccgcggtcg cgcccgccgc
       61 tggatcgagc accgcggcga gctgggtgat gagctgctgg taccacgcca ggccggggat
      121 cgtgcccttg gccggcgcca gcagctcggc gagcgcgcgg aagtccgcca ggctctcggc
      181 catgacctgg tagtacgggc cgtcgccgag caccggggcg gcgttggtcg acaccgtggt
      241 cagaaaccgc aggaaccacg acgacggctg caccatcgcg gtcagcgcga acggcagcgc
      301 ctcctcgctc cccggatcga agcggaagct tccgtagtac gcgcgcagcg cgccggcgta
      361 gcgcgcggcg tagtcgtcga tctgcgcgcg gtagagctcg gtgagctgcg ccagctcctc
      421 gctcggcaga cccaggttgc cggcgcgcgt ggtgatgaag tcgagcgccg gggcgacctg
      481 ctgcgcgaac gccgcggcgg tgtacgtggc ggggagccgg acggtcgggc cctgggcgcc
      541 gctctcggtg ccgtcgccgg gcgcggctcc ggcggggaag aagccgatcg cccgctcggg
      601 atgggcgagg ctctgcgcgt gcacgagggc gatcatcagc gcggcggatg cctcggccag
      661 cttgtggttc cacaccgcca cgtcgaagtc gaacacctcg gggctgccgc cctggacccg
      721 ggacacgcga tagacctcgc ggcccgtggc cggcgcagcc agcatccgct cgaggtcggc
      781 gccgagctcg gcgagggtgc gcggcgaggc cggcgcgagc tggtggtgcg ccaccgccgc
      841 gttgatccct cccagcgcgt cgacgttggc ctccacccac tcggaggtgc cgagcgggct
      901 gtcgaggagc ttcttgatcg acggctgggt ggcgagcgcg agctgggccg acagcagctc
      961 gacgatgctc cggcgcacgg cgtactcgcg cgggtcgtac agctggggcg ggtcgcgatc
     1021 gaggtcatcg agctgcgcct gggacagcgg ctggacctgg tcgtacagcg gcttgatccg
     1081 gccgaacgcg taggactgcc agtcgctgcc ggtgtacacg gggtcgaagg tctcggcgat
     1141 ctcgtactgg taccggctga cgtcgaggta ggtcgacacc acggtcatgg acaggtcgag
     1201 cttggacacc cacagcgcga ggttgtcgcg gatcagcgcg cgcagaccgt tggcctccga
     1261 ggcatagagc agcccgacga tgtacagcat cgtcgagcga ttgatcgtcg ggacccggag
     1321 cttgggcagg atgtactgct cgcgcaggtg gtcggcgaac gtgtcctcga cctgccgctt
     1381 gcgctcgatg aacgtgtggt cgagccacag ccgctcgccg tcgtgcatcc gcccgatcgt
     1441 ggcggcgacg cggcgcgcct ggatctcgcc ggggccgcgc ccgagggtca cctgcgcgtc
     1501 gtagccgtcc acggcctggc cggcggcggc cacctgtccc tggtgccgcc acatcagcgc
     1561 gccgaccacg gccacgcccg ccgccgccgc ggccaggccg gtgcggcgat ggaaccggcg
     1621 ctgctgcgcg atgctcgccg ccaccaggtc atggtcgacg gtgaacggat caccgacgtg
     1681 gttgtccggc gcgagggacg tgaggtacag atccgacggt ggatagtgcg gctgatcggc
     1741 gtcgtcgccg gtgagcgcgt gcagcagcgg agcgagctgg gccaggagca ccgggaacgt
     1801 cgtgtagaag ccgaccagcc gcgcgaagcc gtcgctggtc cggtggacga gcccgtaggc
     1861 caggttgccg tcgaacttcg cgatcagcgc gcgcgccgcg gcggacacgg cgcgggcgtc
     1921 ggtcaggcgg tcgccgagcg cgcggacgtc gagcggaccg tgctgggcgc caaccacggc
     1981 gacgagctcg tcgtagccgt cgatctgatc catgtggctg aggcagaccc ggacggagac
     2041 gccggcgcgg cagcgggcgg ggagcgcgcc gatcttgccg cgcaccagct cggcgagctc
     2101 gcgcagggtc tcgggc
//
LOCUS       DASVOR010001705        10019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_134213,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001705 DASVOR010000000
VERSION     DASVOR010001705.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134213"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VF516_31870"
     CDS             <1..524
                     /locus_tag="VF516_31870"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8112386.1"
                     /translation="GTDVCGIATHRVCSCGVAIVPEGRRVFAHMTVHDNLCLGAYRPA
                     ARRGYQEQLARAYELFPRLSERSGQLAGTLSGGEQQMVAIARALMARPRLLLLDEPSL
                     GLAPVVIDEVFDALARIGAQGVSILLVEQDVERALDLATRAYLLVEGRIAAEGSSAEL
                     RQSDAVRAAVLGT"
     gene            complement(672..2567)
                     /locus_tag="VF516_31875"
     CDS             complement(672..2567)
                     /locus_tag="VF516_31875"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HEX8112387.1"
                     /translation="MFARSRSLQCVAGVLALCASSAAVADTAYITNDGDGTTPSTVSV
                     IDTSTNTVIRSITVGLRPRTIAANPQNRMVYVANEGSSFVGGDPGSVSVIDARTSTVV
                     ATIPINAPYGIAASGQRVYVSGFSNSVTVINAVNNAVIATIPIPGGCCGVGAEPLAIV
                     ATPDGSRVYTANFIDSSVSVIDTASLTRIATVAIPAGDQPQAIAVNPAGTRIYTANLN
                     SLTVIDVASNTVVATIPATPANGLAFPQGIAVNPAGTLVYATNSIGSSISVFSTATNG
                     LVTVIPAGSGPEGISFTSDGRRAYVADKDTNVVHVIDTASNTLLTDVAVGATPISTGS
                     FILPGCTTHHRLDFNGNGTTDVAVFRQPTGQWFNVESFNVVFGVANQHDIPVAEDYDG
                     DCKADNAYWNPVTGTWNVILSTTGATISQQWGAANLGDIPEPGDYDGDGKTDFAVFRQ
                     STGQWFLFQSANGINNPVFGVANEHDIPAAGDYDGDGKTDLAYWNPATGNWNIRFSSS
                     GATFTRNWGLGSVGDVPVPGDYDGDGKTDLAVFRQPTGQWFIILSSNNSFFQPVFGVA
                     GQNDIPVPGDYDGDGRSDVAYWDPASGNWNIRFSVSGATTVRNWGLGSVGDIPLSAPP
                     AYIRSHF"
     gene            3090..4190
                     /locus_tag="VF516_31880"
     CDS             3090..4190
                     /locus_tag="VF516_31880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112388.1"
                     /translation="MRSWLSALTLLAGCGDNLGPPFDHTVDAADVSILYPLPDTTDLL
                     IQPAEQGAFGQLFPERLFPTAIGPVDVGTTYADVRLIALRLDPCSARRTCNSEVRAIF
                     QPVVVGPDGKPTVADGANHVFYAMPKAELVSFLKQILRLKELYGAGIAYGSTLGPQPI
                     LVATGLDGDFARGLHALVLEHLGESRIERFTEFNHQIPGRDRWDFYLFDGADLARRTI
                     ATTSTDEQQVTGTHTDPMNAGGIVMVSPALDSPLSALVDENRPADATDPIRAGFGRAI
                     ELQDPRKENSESVDCVSCHLAEGARRIGAAQYGLSSSAGFQSDRSLDYQRDLGAVTNL
                     HMFAYDGRYVSVTQRVANESAVVADALQELVR"
     gene            4395..5840
                     /locus_tag="VF516_31885"
     CDS             4395..5840
                     /locus_tag="VF516_31885"
                     /inference="COORDINATES: protein motif:HMM:NF020416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase/BAAT C-terminal
                     domain-containing protein"
                     /protein_id="HEX8112389.1"
                     /translation="MIILLAACGNDRGAILPIPPPAAPHFDILPGAEALSGDPLEIAL
                     GGLAPGSEVEIVAERPVTAAWAGGKRALYRAHATFRVGADGRLDLARDAPRSGTYDGA
                     DLRGLFWSMAPIEAPVSDGWPDDRIQLVATQRGTAAARATVTLQSADPKVEMTPVGAE
                     LPGAVLAHLPGSERRPAIIVLGGSEGGDWFAREMAPRLASHGYAVLGVPYYAPPFATP
                     HADLKQLPPAFVDIPVDRLERARDWLRGQAGVDAERIAVYGVSKGAEFALLAATRLSW
                     ITAAVAIVPSDVVWEGWGLPGQVPGTRSCFSWRGEALPFVPYEGFAEEFARFGSGGKV
                     HMRRPQDAGRAAHPDAVAKARIPIETYRGPVLVAGGGDDQVWASGEMAEHIARSRAAV
                     GLPTTLRVYPDAGHLLGGDGWSPTTQIDAAPFAVGGTPAGTARAQAEVWRETLAFLAR
                     ALSTRRADAGPARASLRRGGPQDLAWRYIVQ"
     gene            5853..6920
                     /locus_tag="VF516_31890"
     CDS             5853..6920
                     /locus_tag="VF516_31890"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEX8112390.1"
                     /translation="MIRRRAFLYWLCQIGGWFSWTAIGVLFAVAGPSSPFESAWWRNV
                     LAFSSVPVASIAWTHGYRRFLRRRGWTGLGPGKLLPRVLVASMILGVVIPYTAAPVYL
                     ILYPERAHIFGMWVLPAVGSSIWCVVIWNVAYFGIHYFERWRNAERDKLQLAVVAAEA
                     KLHGLMSQLNPHFLFNCLNSVRALIVEDPAKAQAAVTALSELMRYSMQASRIATVPLE
                     TELEMVRTYLSLEEVRLDERLTSRFEIAAEAHAVPIPTMLVQLLVENGVKHGIERLPA
                     GGAIGVAAWLGDGLLHVRVTNSGQIGPHHGSTRVGLANARERLRLLYGERASLALRED
                     GHAVIAELALPVAPPAAVGSA"
     gene            6917..7633
                     /locus_tag="VF516_31895"
     CDS             6917..7633
                     /locus_tag="VF516_31895"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEX8112391.1"
                     /translation="MRALIIDDERLARNELRRLLESIRDVEIVGEAKNADDAIEQIGR
                     LHPDLLFLDVQMPGCDGFELLERLDEAPRVIFTTAFDRYALRAFDVSALDYLVKPIAP
                     DRLAAAVAKAAARSPAAPAADPSGPLGRGQRIFVRDGERCWFVAVEEIVLIESEGNYA
                     RLHFAGHRPLLPRSLNAIGDRLDPALFFRANRRQIINLERIETLAPWPNDGYLVKLSG
                     GLEVEMSRRQARLFHAKARL"
     gene            7923..9152
                     /locus_tag="VF516_31900"
     CDS             7923..9152
                     /locus_tag="VF516_31900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112392.1"
                     /translation="MKRMIVSCAASYLVVFAAGCSENAFEPSGTEDPLVSDTGTIPVD
                     LKSQSEHLPTPAATTRTMLYFGGPVISNPKVYVVWWGSAASINSILTAARGGIADFYA
                     GVTNSNYLDWWNEYDTNITVQSGSHAGTAGTGQHIGRGNLVGTRTLTPVPAGNVTDTQ
                     IQATLDQAFTNGTLPQPDANTIYAIHFPRSVTITLDGSKSCSAFGGYHFNTVETTRHN
                     AYYIVIPDCGNSFRSFTIVNSHELAEATTDAQPTPGSNPDFPQAWNNATGSEVGDLCQ
                     GTSGSVTTALGTFTVQGIWDEPSHGCPVFRSNTSDYNVSFATPSATLAAGGQITATVR
                     TATVRGTAQTLTLSVTAPAGVTATISPTQVTSGASATLTVSASSAVTAAQVIVSAVGT
                     TGSAAQTHTASLLLNGG"
     gene            complement(9139..9891)
                     /locus_tag="VF516_31905"
     CDS             complement(9139..9891)
                     /locus_tag="VF516_31905"
                     /inference="COORDINATES: protein motif:HMM:NF027806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112393.1"
                     /translation="MTRPLGYRLGPWIALGWILLCGHVAWAHGNGADPGKSDAAPART
                     APYDPDQAKAASQISDPEAATRAYLDAVSPERRAQTRSYAAGRYVLHAVDFVVSSAVM
                     ILLIALGISVRFRNLARRITRIRALQTALYWIQLSIAVTVIQFPLTIYAEYYREKHYD
                     LLTQSFSGFLGDQAKSLLIGCIIGALLRGHHVLEARRVPRPRSRPGDRGDLLRSPVGP
                     VPDPQRHGVEARARPRRSMTARRRGSWVFSRR"
BASE COUNT         1491 a         3513 c         3417 g         1598 t
ORIGIN      
        1 gcggcacgga cgtgtgcggc atcgccacgc accgggtctg ctcctgtggc gtggcgatcg
       61 tgcccgaggg ccggcgcgtg ttcgcgcaca tgaccgtgca cgacaacctc tgcctgggcg
      121 cctaccggcc ggcggcacgg agaggctacc aggagcagct ggcgcgggcc tacgagctgt
      181 tcccgcgcct ctcggagcgc tcgggtcagc tcgccggcac gctgtcgggc ggcgagcagc
      241 agatggtggc catcgcccgg gcgctcatgg ccaggccgcg cctcctcctg ctcgacgagc
      301 cgtcgctcgg cctcgccccc gtcgtcatcg acgaggtgtt cgacgccctg gcccggatcg
      361 gcgcccaggg ggtcagcatc ctcctcgtcg agcaggacgt cgagcgggcg ctcgacctgg
      421 ccacgcgggc ctacctcctg gtcgagggca ggatcgccgc agaggggtca tcggccgagc
      481 tccgccagag cgacgccgtg cgcgccgcgg tcctcgggac ctgagcgaga ccgcgctcga
      541 gggcgtccga gaaagcgggc ttgcggccga ccgcttcgcg tgcccggacc gcccaggtgc
      601 gcggtccggg catcgggatg ccttgcccca cagcccgggc gcgaggccgg ccacacgatg
      661 gagaacccgg atcagaagtg cgagcggata taggccggag gcgccgacag cgggatatcg
      721 ccgacgctgc cgaggcccca gttgcggacg gtcgtcgcgc cgctgacgct gaaccggatg
      781 ttccagttac cggacgccgg atcccagtac gcgacgtcgc tgcggccgtc accgtcgtag
      841 tcgcccggca ccgggatgtc gttctgcccg gccactccga ataccggctg gaagaacgag
      901 ttgttgctgc tcaggatgat gaaccactgg ccggtgggct ggcgaaacac cgcgagatcg
      961 gtcttgccgt cgccgtcgta gtcgccaggg accgggacat caccgacgct gccgaggccc
     1021 cagttgcgcg tgaaggtcgc accgctggag ctgaaccgga tgttccagtt gccggtggcc
     1081 gggttccagt aggcgagatc ggtcttgccg tcaccgtcgt agtcgcctgc cgccgggatg
     1141 tcgtgctcgt tcgcgacgcc gaacacgggg ttgttgatgc cgttcgccga ctggaacagg
     1201 aaccactggc cggtgctctg gcggaacacg gcgaagtcgg tcttgccgtc gccgtcgtag
     1261 tcgccgggct ccgggatgtc gccgaggttc gccgcgcccc actgctgact gatggtcgcg
     1321 ccggtggtgc tgaggatgac gttccaggtg ccggtcaccg gattccagta ggcgttgtcg
     1381 gccttgcagt cgccgtcgta gtcctcggcg accggaatat cgtgctggtt ggcgacgccg
     1441 aataccacat tgaagctctc gacgttgaac cactgcccgg tcggctggcg gaacaccgcg
     1501 acgtccgtgg tgccgttgcc gttgaagtcg aggcggtgat gcgtcgtgca gccgggcaag
     1561 atgaacgacc cggtggagat cggcgtggca ccgaccgcaa catcggtcaa cagcgtgttg
     1621 ctcgccgtgt cgatcacgtg caccacgttg gtgtccttgt cggccacgta ggcgcgccgt
     1681 ccgtcgctgg tgaacgagat gccttcgggg cccgagccgg ccgggatgac ggtgaccagg
     1741 ccgttggtcg cggtgctgaa caccgagatc gagctgccga tcgagttggt cgcatagacc
     1801 agggtgccgg cggggttgac cgcgatgccc tggggaaacg ccaggccgtt cgcaggggtc
     1861 gccgggatcg tcgcgaccac cgtgttgctg gcgacatcga tcacggtcag cgagttgagg
     1921 ttcgcggtgt agatccgcgt gcccgccggg ttgaccgcga tcgcctgggg ctggtcaccc
     1981 gccgggatcg cgaccgtcgc gatccgcgtc agcgacgcgg tgtcgatgac cgagaccgag
     2041 ctgtcgatga agttggccgt gtacacgcgc gaaccgtcgg gcgtggcgac gatggccagc
     2101 ggctcggcgc cgacaccgca acacccgccg gggatcggta tcgtcgcgat cacggcattg
     2161 ttgaccgcgt tgatcacggt caccgagttg ctgaagccgg acacgtagac gcgctgaccg
     2221 gacgcggcga taccatacgg cgcattgatc gggatggtcg cgaccacggt gctggtccgg
     2281 gcgtcgatca ccgagacgct gcccgggtcg ccgccgacga acgagctgcc ctcgttggcg
     2341 acgtagacca tgcggttctg cgggttggca gcgatggtcc gcggtctcag cccgacggtg
     2401 atggagcgga tcaccgtgtt cgtgctggtg tcgatcaccg acacggtgct gggtgtggtg
     2461 ccgtcgccat cattggtgat gtatgccgtg tccgcgacgg cggcgctgga cgcgcacagc
     2521 gcaagaacgc cggcgacgca ctgcaaactt ctcgatctgg caaacattgc tactcctgct
     2581 aaagttgacg gtcggagcgt agtttcgtcg atgcgccgtc aacccgcgtg ccgcattgcg
     2641 ctgcacagag ccacaccttc tggggctcct ccctcagcct cggctgtggc gccggcgacc
     2701 tgcgccacac cgcagacctc gcttgtgcca cttcgagggg cacatgctct tcgcttgtgc
     2761 catttcgcag acgcgcaatg atctgatggg tcgccgctca tggatggcat cgcgcttgct
     2821 ccgtgcgaca agcggccggt caccgcgtcc atgatacgaa gcgtacagct accgtcgaga
     2881 gcgtgcgagc ttcgtgcctc acctgctggg aagcggactg ctggcatcca aggaggtaga
     2941 acgggcactt atgagccgtt cgtcgctccg gatgcgtgac ggcgagcacg cgccgcgggt
     3001 ggtgcgccgg gtacccgacg gatggaaccg gtgtagcgtg cggttgcgcc ggcaccgagg
     3061 tcggccttcc atgatagcat atgtacaaca tgcggagttg gctctcggcg ctcacgctcc
     3121 tggctggctg tggcgacaat ctcggaccgc cgttcgacca caccgtcgac gctgcggacg
     3181 tatcgatctt gtatccactc cccgacacga cagatctgct gatccagcct gccgagcaag
     3241 gcgcgtttgg ccagctgttt cccgaacgct tgtttccgac ggcgatcgga ccggtcgacg
     3301 tcgggacgac ctacgccgac gtgcgcctga tcgcgctgcg gctggatccg tgttcggcgc
     3361 gcaggacctg taacagcgaa gtgcgcgcga tctttcaacc cgtcgtcgtc ggcccggatg
     3421 gcaagccgac ggtcgccgac ggtgcgaacc atgtgtttta cgccatgccg aaggcggagc
     3481 tggtgtcgtt cctgaagcag atcctgaggc tgaaggaact gtacggagcc ggcatcgcgt
     3541 atggcagcac gctcggtccc cagccgatcc tcgtcgccac ggggctcgat ggtgattttg
     3601 cccgcgggct ccatgcgctc gtgctcgagc atctcggcga gtcgaggatc gagcgcttca
     3661 ccgagttcaa tcaccagatc ccgggccgtg accgctggga tttctatctg ttcgacggcg
     3721 ccgatctcgc tcgccgcacc atcgccacca ccagcaccga cgaacaacag gtcaccggca
     3781 cgcacaccga tccgatgaac gcaggtggta tcgtcatggt gtcaccggcg ctggactcac
     3841 cattgagcgc gctcgtcgac gagaatcgtc cggccgacgc caccgacccg atccgcgccg
     3901 gtttcgggcg cgcgatcgag cttcaggatc ccaggaagga gaactcggag tccgtcgatt
     3961 gcgtctcgtg ccatctggcc gaaggcgcgc gacggatcgg tgccgcccag tacggtctct
     4021 cctcctccgc tggctttcaa agcgatcgca gcctcgacta ccagcgcgac ctcggcgccg
     4081 tcacgaatct acacatgttc gcgtacgacg gtcgctacgt gtcggtcacg caacgtgttg
     4141 cgaacgagtc ggcggtcgtc gcagacgctc tgcaagaact ggtgaggtag accggccctc
     4201 gaggacctga tcggcagcgt ccgcccgccg tcggcgcaga cccaccggtt cggtccaatc
     4261 catccggcca tccggccccg cggatcgacc gcccatccag ccccggggcc gctcatcccg
     4321 atccccgccg cggactcgcc gggtgcggct acaccgcggt catgcattca cgacagatcc
     4381 ggacgggcgg cgcggtgata atcctcctgg cagcatgcgg caacgaccgc ggcgcgatct
     4441 tgccaatccc gcccccggcc gcccctcact tcgacatcct cccgggcgcc gaggcgctgt
     4501 ccggagaccc gctggagatc gcgctgggag gcctcgcgcc cggcagcgag gtcgagatcg
     4561 tggccgagcg cccggtcacg gccgcctggg ccggcggcaa gcgcgcgctc taccgcgccc
     4621 atgccacctt ccgggtcggt gccgacggcc ggctggatct cgcccgcgac gcgccgcggt
     4681 ccggcaccta cgacggcgca gacctgcgag ggctgttctg gtcaatggct ccgatcgagg
     4741 ccccggtctc cgacggctgg ccggacgacc ggatccagct cgtggcgacg cagcgcggga
     4801 cggccgccgc ccgggcgacc gtcaccttgc aaagcgccga tcccaaggtc gagatgacgc
     4861 ccgtcggggc ggagctcccg ggcgccgtgc tcgcgcatct cccggggtcg gagcgccggc
     4921 ccgcgatcat cgtgctcggc ggctccgagg gcggcgactg gttcgcgcgc gagatggcgc
     4981 cgcgactggc gtcgcacggc tacgcggtgc tcggcgtgcc ctactacgcg ccgccctttg
     5041 cgacccccca cgccgacctg aagcagctcc cgcccgcgtt cgtcgacatc ccggtcgacc
     5101 gcctcgagcg cgcgcgcgac tggctccgcg gccaggcggg cgtcgacgcg gagcgcatcg
     5161 cggtctacgg cgtctccaag ggcgccgagt tcgcgctgct cgcggcgacc cggctgtcgt
     5221 ggatcaccgc ggccgtcgcg atcgtcccca gcgacgtggt gtgggagggc tggggcctcc
     5281 cggggcaggt gcccggcacg cgctcgtgct tctcgtggcg tggcgaggcg ctgccgttcg
     5341 tgccgtacga gggcttcgcc gaggagttcg ctcgcttcgg gtccggcggc aaggtccata
     5401 tgcgcagacc ccaggacgcc ggccgcgcgg cacaccccga cgcggtggcc aaggcgcgca
     5461 ttccgatcga gacctatcgc gggccggtcc tcgtcgccgg cggcggcgac gaccaggtct
     5521 gggcctcggg cgagatggcc gagcacatcg cgcggagccg cgctgcggtt ggtctcccca
     5581 cgaccctgcg ggtgtatccg gacgccggcc acctgctcgg cggcgacggc tggtcgccga
     5641 ccacgcagat cgacgccgcg ccgttcgccg tcggtggcac ccctgccggc accgcgcggg
     5701 cccaggccga ggtgtggcgc gagacgctgg cgttcctggc gcgagcgctg tcgacccggc
     5761 gcgccgacgc cggccctgcg agggcgagcc tccggcgcgg tggaccgcag gacctggcct
     5821 ggcgctatat tgtgcagtga cgcgaggcgc gtgtgatccg caggcgagca ttcctctact
     5881 ggctgtgtca gatcggtggc tggttctcct ggaccgcgat cggtgtgctg ttcgccgtcg
     5941 ccggtccgag cagcccgttc gagagcgcct ggtggcggaa cgtcctcgcg ttcagctccg
     6001 tcccggtggc ctcgatcgca tggacgcatg gctaccggcg cttcctccgg cgccgcggct
     6061 ggacaggcct cggccccggc aagctcctgc cgcgcgtgct cgtggccagc atgatcctcg
     6121 gcgtggtgat cccctacacc gccgcgccgg tctacctgat cctgtacccg gagcgcgcgc
     6181 acatcttcgg catgtgggtc ttgcccgcgg tggggagctc gatctggtgc gtcgtcatct
     6241 ggaacgtcgc ctacttcggc atccactact tcgagcgctg gcgcaacgcc gagcgcgaca
     6301 agctgcagct cgcggtggtc gccgccgagg ccaagctcca cggcctgatg tcgcagctca
     6361 acccgcactt tctgttcaac tgcctcaaca gcgtgcgcgc gctcatcgtc gaggatccgg
     6421 ccaaggccca ggccgccgtc acggcgctgt ccgagctgat gcgctactcg atgcaggcca
     6481 gccggatcgc cacggtgccg ctcgagaccg agctcgagat ggtcaggacc tacctctcgc
     6541 tcgaggaggt ccggctcgac gagcgactga ccagccggtt cgagatcgcc gccgaggcgc
     6601 acgcggtgcc gatcccgacc atgctggtcc agctcctggt cgagaacggg gtcaagcatg
     6661 gcatcgagcg cctgcccgcc ggaggcgcga tcggcgtcgc ggcgtggctg ggcgacggcc
     6721 tcctgcacgt ccgggtcacc aacagcgggc agatcgggcc gcatcacggg tcgacccggg
     6781 tcggcctggc caacgcgcgc gagcgcctgc gcctgctcta cggcgagcga gcgagcctgg
     6841 cgctgcgcga ggacggccac gcggtgatcg ccgagctggc cttgccggtg gcgccgcccg
     6901 ccgcggtggg cagcgcatga gggcgctgat catcgacgac gagcgcctgg cccgcaacga
     6961 gctgcggcgg ctgctcgagt ccatccgcga cgtcgagatc gtcggtgagg ccaagaacgc
     7021 cgacgacgcg atcgagcaga tcggccggct gcaccccgac ctgctgttcc tcgacgtgca
     7081 gatgcccggc tgcgatgggt tcgagctgct cgagcgcctc gatgaagcgc cgcgggtgat
     7141 cttcaccacc gcgttcgacc gctacgcgct ccgggcgttc gacgtcagcg cgctcgatta
     7201 cctggtcaag ccgatcgcgc cggaccgcct cgccgccgcc gtggccaagg ccgccgcgcg
     7261 ttcccccgcc gcccccgccg ccgatccttc cggaccgctc ggccgcggcc agcgcatctt
     7321 cgtgcgcgac ggcgagcgct gctggttcgt cgcggtggag gagatcgtgt tgatcgagtc
     7381 cgagggcaac tacgcgcggc tccacttcgc cggccatcgc ccgctcttgc cgcgatcgct
     7441 caacgcgatc ggcgaccgcc tcgaccccgc gctgttcttc cgcgccaacc ggcgccagat
     7501 catcaacctc gagcgcatcg agaccctcgc gccgtggccc aacgacggct acctggtcaa
     7561 gctgagcggc ggcctcgagg tcgagatgtc gcgccgtcag gcccgcctgt tccacgccaa
     7621 ggcgcggctg taacccctcg ggcccgtcca cttgcgtgtg gagtcttcgc ggcgcctttg
     7681 tcgacgatcc ggagtgcagc gtgagctgag catgggctga ccgatggccg cacactcgct
     7741 tacactgcca actcccgcgg agctcgatac tcccgcctga cgttgcgcgc ggtgcctcgt
     7801 ggcatttcgc ccgagcgagc aaagccggct tgctgtcctg gccctggccg ctcgaaacat
     7861 gataaaggct tcgagcttca cgcgcccccg cgccgggggc gccgggccca gagggagttg
     7921 ccatgaagag aatgatcgtc tcgtgtgctg cctcgtatct cgtggtcttt gccgccggct
     7981 gctcggagaa cgccttcgag ccgtccggga ccgaggaccc gctcgtctcc gataccggga
     8041 ccatcccggt cgacctcaag tcgcagtcgg agcatctgcc cactccggcc gcgaccacga
     8101 ggacaatgct gtacttcggc ggaccggtga tctcgaaccc gaaggtctat gtcgtgtggt
     8161 gggggagtgc tgccagcatc aactcgatcc tcaccgccgc gcgcggaggc atcgccgact
     8221 tctacgccgg tgtcaccaac agcaactacc tggactggtg gaacgagtac gacacgaaca
     8281 tcacggtaca gtcgggctcg cacgccggca cggcgggcac gggccagcac atcgggcgcg
     8341 gaaacctcgt cggcacccgc acgctcacgc cggtccccgc gggcaacgtc acggacaccc
     8401 agatccaggc aacgctcgac caggcgttca ccaatggcac gctgccccag cccgacgcga
     8461 acacgatcta cgcgatccac ttcccgagga gcgtcacgat cacgctcgac ggcagcaaga
     8521 gctgctctgc gttcggcggg taccacttca atacggtcga gaccacccgg cacaacgcgt
     8581 actacatcgt gatccccgac tgcgggaact cgttccgcag cttcacgatc gtcaactccc
     8641 acgagctcgc ggaggcgacc accgacgctc agcccacgcc cgggtcgaac ccggacttcc
     8701 cccaggcgtg gaacaacgcg accgggagcg aggtcggcga cctgtgccag ggaacgtcgg
     8761 gcagcgtgac gaccgccctc gggacgttca ccgtgcaggg aatctgggat gagccgtcgc
     8821 acggatgccc ggtgttccgc tccaacacga gcgactacaa cgtgtcgttc gccacgccgt
     8881 ccgcgacgct cgccgccggt ggccagatca cggccaccgt tcgcacggcc acggtgcggg
     8941 gcaccgcgca gacgctcacg ctctcggtga ccgcgccggc cggcgtcacg gcgacgatca
     9001 gcccgacgca ggtcacgtcc ggcgcgagcg cgacgctcac cgtgtcggcg agctccgcgg
     9061 tgaccgcggc gcaggtcatc gtctcggccg tcgggacgac cggctcggcc gcgcagacgc
     9121 acaccgcgtc gctcttgctc aacggcggct gaacacccag gatcctcgtc gccgtgccgt
     9181 catcgagcgc cggggccgtg ctctcgcttc cactccatgg cgttgcggat ccgggaccgg
     9241 cccgacgggt gatcgaagaa gatcgcctcg atcaccgggt ctggatcgag gtcgcggtac
     9301 tcgccgagct tcaagaacgt ggtggccgcg cagcagcgcg ccgatgatgc agccgatcag
     9361 cagcgacttc gcctggtcgc cgagaaaccc cgagaagctc tgggtgagca gatcgtagtg
     9421 cttctctcgg tagtactctg catatatcgt caatggaaac tggatcacgg tgacggcaat
     9481 agacagctgg atccagtaca gcgccgtctg cagcgcgcgg atccgggtga tcctgcgcgc
     9541 caggttgcga aaccggaccg agattcccag cgcgatgagc aagatcatga ccgcgctgct
     9601 gaccacgaag tccaccgcgt gcagcacgta ccgcccggcg gcgtaggacc tggtctgcgc
     9661 cctccgctcg ggcgacaccg catcgaggta cgcgcgggtt gcggcctcgg ggtcggagat
     9721 ctgggaagcg gccttcgcct ggtcaggatc gtacggtgcc gtcctggccg gcgcggcgtc
     9781 ggacttgccg ggatcggcgc cgttgccgtg cgcccacgcg acgtggccgc acagcaggat
     9841 ccatcccagc gcgatccacg gtccgaggcg atatcccagc gggcgtgtca tgtgccgacg
     9901 aggatgcatc gcctgccgtg gccccgcaac gccggtgtca ttgccgggcg cgcccacgcc
     9961 gagcgccgcc gcgcgccgac ggcgccgtgg tcaggcgcgc aggccttgcg cgtcgggct
//
LOCUS       DASVOR010001706         2552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_455629,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001706 DASVOR010000000
VERSION     DASVOR010001706.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2552
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455629"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..620)
                     /locus_tag="VF516_31910"
     CDS             complement(183..620)
                     /locus_tag="VF516_31910"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8112394.1"
                     /translation="MRVVVDHVAGSRRGQRQEFAAGVRLRFGRHPECEVSFDPHRDID
                     ASSRHAELRQVDAGWVLVDLGSSNGTYVDGHRVTETPIVRNLPVAVEFGPGGPRIRLF
                     IGDDKAIEALPPAPLEAARPTWLVPVTAGAIIVVVILLFALRC"
     gene            complement(634..1782)
                     /locus_tag="VF516_31915"
     CDS             complement(634..1782)
                     /locus_tag="VF516_31915"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEX8112395.1"
                     /translation="MSEPVPLESLAIVVAGASDVGQVREHNEDHFLVGDLDSMLPVDV
                     AEPWSATCLRGPLVVVCDGMGGVEGGEIASELAALVTWREMKRTPDTRDPEVFARLLR
                     RAVRVANHDVHAMARREPGLRGMGTTLSAAGVVGDRLILATVGDSRAYVLRSGALVQV
                     TRDQSLQWALLAAGHSPNEAASAGGAILQALGVADDVEPSLSIIELRRGDRVLLCSDG
                     LHGLVGDPALALLLSEPHTVAESVQLLIAAARAAGGSDNITAVVCELSGPRLLTPSGE
                     SDLPSFREFDPQQEGDPALTPTSYVARRLAARIGLPTASIPPSIPVTGQHAVLRSQIE
                     IRLPATALPLEEVEGPARRRLRVAGLTWWPYVVIAVIACAIAWWLGGR"
     gene            complement(1784..>2552)
                     /locus_tag="VF516_31920"
     CDS             complement(1784..>2552)
                     /locus_tag="VF516_31920"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8112396.1"
                     /translation="PLAAKLDGPIDPHAGTLAQDEASAASALHVPPPPRLPPGTWGMG
                     VLVNRDGSDGQRYPLAGEYLIVGRAGADIAFEDDRFLARQHARLERGGDGVVRVHPLD
                     ALNGVFRKTDAPIELTDGMTILVGREVLRYERLDPEEIKVYPLVRHGVALFGSPPREP
                     WGRLVQLVPSGGYRDVRHLAGEEVVLGREEGDIVFRDDAFLSRRHAALTWDGKRAQIT
                     DLGSSNGTFVRIIGPTALRHGDHLRMGDQLLRIELGR"
BASE COUNT          381 a          930 c          932 g          309 t
ORIGIN      
        1 tccggtgccc ggtcccgcgc gccggttggg tgccagtcga tagccgcaca cgccgcagaa
       61 ccgcgcattc ggctcgagcg gcgaattgca cgacgggcac gtcatgtcga cgaattttcg
      121 cagaacatcc cagccttggc acgcgacatt ccagcaaaac gccgccagca aaacgctcgg
      181 gcctagcagc gcagcgcgaa cagcaggatc acgacgacga tgatcgcgcc cgcggtcact
      241 ggcacgagcc atgtaggccg ggcggcttcg agcggcgccg gcggcaaggc ctcgatcgcc
      301 ttgtcatcgc cgatgaacag ccggatgcgc ggaccgccgg ggccgaactc caccgcgacc
      361 gggaggtttc gcacgatcgg ggtctcggtc acgcgatggc cgtcgacgta ggtgccgttg
      421 gagctcccga ggtcgaccag cacccacccc gcgtcgacct ggcgcagctc ggcatgacgc
      481 gagctggcat cgatgtcgcg gtgcggatcg aacgacacct cgcactcggg gtggcggccg
      541 aaccgcaggc gcactccggc tgcgaactcc tggcgctggc cccggcggct gccggcgacg
      601 tggtcgacca cgacccgcac gggagcaatg gtatcaccgg ccgccgagcc accacgcgat
      661 cgcgcacgcg atgacggcga tcaccacgta cggccaccag gtcaggccgg ccacccgcag
      721 ccggcggcgc gccgggccct cgacctcctc cagcggcagg gcggtcgccg ggagtcggat
      781 ctcgatctgc gagcgcagca cggcgtgctg gccggtcacg gggatcgacg ggggaatcga
      841 cgcggtcggc aggccgatcc gcgccgcgag gcggcgcgcc acgtacgagg tcggggtcag
      901 ggcgggatcg ccctcctgct gcgggtcgaa ctcacggaaa ctcggcagat cgctctcacc
      961 ggacggggtc aggagccggg gaccggacag ctcgcacacc accgccgtga tgttgtcgct
     1021 cccccccgcg gcgcgcgcgg cggcgatcag cagctggacc gactcggcca cggtgtgggg
     1081 ctcggacagc aagagcgcga gcgccgggtc gccgaccagg ccgtgcagcc catcgctgca
     1141 cagcagcacg cgatcgccgc ggcgcagctc gatgatcgac agcgacggct cgacgtcgtc
     1201 ggcgacgccg agcgcctgga ggatcgcgcc gccggcgctc gcggcctcgt tggggctgtg
     1261 gccggccgcg agcagcgccc actgcagcga ctggtcgcgc gtgacctgga ccagcgcgcc
     1321 gctgcgcagc acgtaggccc gggagtcgcc gacggtggcg aggatcaggc gatcgccgac
     1381 cacgccggcc gccgacagcg tcgtgcccat gccgcgcaac ccgggctcgc ggcgcgccat
     1441 cgcgtggaca tcgtggttgg cgacccggac ggcgcggcgc agcaggcggg caaacacctc
     1501 cgggtcccgg gtgtccggcg tgcgcttcat ctcgcgccag gtcaccagcg ccgcgagctc
     1561 ggacgcgatc tcgccgccct cgacgccgcc catgccgtcg cacaccacga ccagcgggcc
     1621 gcgcaggcag gtcgccgacc acggctcggc gacgtcgacc ggcagcatgc tgtcgagatc
     1681 gccgaccagg aagtggtcct cgttgtgctc gcggacctgg ccgacatcgc tggccccggc
     1741 gaccacgatg gcgagcgact cgaggggaac cggttcgctc acgtcacctg ccgagctcga
     1801 tccgcagcag ctgatcgccc atgcggaggt ggtcgccgtg ccgcagcgcg gtcggtccga
     1861 tgatgcggac gaaggtcccg ttcgagctgc cgagatcggt gatctgcgcg cgcttgccgt
     1921 cccaggtcag cgcggcgtgg cgccgcgaca ggaacgcgtc gtcgcgaaac acgatgtcgc
     1981 cctcctcgcg gccgagcacg acctcctcgc cggccagatg gcggacatcg cggtagccgc
     2041 ccgacggcac gagctggacc agccggcccc acggctcgcg cggcggcgag ccgaacagcg
     2101 cgaccccgtg gcggaccagc gggtagacct tgatctcctc gggatcgagc cgctcgtagc
     2161 gcagcacctc gcggccgacg aggatcgtca tgccgtcggt cagctcgatc ggcgcgtcgg
     2221 tcttgcggaa cacgccgttg agcgcgtcga gcgggtggac cctgaccacg ccatcgccgc
     2281 cgcgctcgag ccgggcgtgc tggcgcgcga ggaagcgatc gtcctcgaac gcgatgtcgg
     2341 cgccggcgcg gccgacgatc aggtactcgc cggcgagcgg gtagcgctga ccgtcggagc
     2401 cgtcgcggtt gaccagcacc cccatgcccc aggtcccggg cggaagccgc ggcggcggtg
     2461 gcacgtggag cgccgaggcc gccgaggcct cgtcctgggc gagcgtgccg gcgtgcgggt
     2521 cgatcgggcc gtcgagcttg gcggcgagcg gc
//
LOCUS       DASVOR010001707         2248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_222121,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001707 DASVOR010000000
VERSION     DASVOR010001707.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222121"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2248
                     /locus_tag="VF516_31925"
     CDS             <1..>2248
                     /locus_tag="VF516_31925"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8112397.1"
                     /translation="AAPAAADPASSLHRLMQQQLEAMSALVRQQLEVVASASGIAASA
                     PAPALPPDDDPPAGGRNPFGMDRFSEPPREMAPRQQEHIERLSRRLSARTGGSMRYAA
                     EHRGRWADLRTAMTFRPETKAMCYPIVGDRSRGARVWDVDGNEYVDVSMGFGINLFGH
                     NPRFVTEAVRRQLERGMHVGIQSDLAGQVAERLAALTGLQRTTFCNSGTEAVMTAVRM
                     ARAVTGRTRVVQFSGSYHGHSDVTLVVAGARDGAGAPMAAGVPGQTARQSLVLPYGEE
                     RSLRMIDEHLGELAAVLVEPVQSRRPDLQPVDFLRRLREMTRRAGVPLIFDEVLTGLR
                     AHPNGAQGLFGIDADLATYGKILGGGMPIGVVAGHPRFLDAVDGGSWRFDDRSAPRAE
                     MTFVAGTFCKHPLAMAASLAVLDHLIAEGPALQADLNRRTAELVERLCAVLAEEGAPI
                     AVHRFASLFRFAAAQNSSYLFQPLEMDLFYYGLIERGVYVWEGRTCFLSTAHTDADLD
                     HLVEAVRGSVRELIAAGFFAEAGRAPVRRAEVADEVTDEVTDERPLTEAQQQLATLAR
                     MTAGGSSAYNVATSLELRGALDTAALGAALGDVVRRHEALRTVLTADGERQRILPSLE
                     IPLETDLETVGLDGDAGAAAVDAWLLAENARPFDLEAGPLLRARLLRLAPDRHLLAVV
                     AHHLVVDGWSMEVVVRDLLQRYGARVTGAPAPAPPSMQWRDYLAWQAAQAAQPAMAAH
                     EAYWHELLA"
BASE COUNT          276 a          883 c          785 g          304 t
ORIGIN      
        1 agctgcgccc gccgcggccg accccgccag cagcctgcac cggctgatgc agcagcagct
       61 cgaggcgatg tcggcgctgg tccgccagca gctcgaggtc gtcgccagcg catcgggcat
      121 cgccgcgtcc gcgcccgcgc ccgcgctccc ccccgacgac gatccgccgg ccggcggccg
      181 caacccgttc ggcatggacc gcttctccga gccgccgcgg gagatggcgc cgcgccagca
      241 ggagcacatc gagcgcctgt cgcgccggct gtcggcccgc accggcgggt cgatgcgcta
      301 cgccgccgag catcgtgggc gctgggccga tctgcgcacg gcgatgacgt tccgccccga
      361 gaccaaggcg atgtgctacc ccatcgtcgg cgaccgctcc cggggcgcgc gggtctggga
      421 cgtcgacggc aacgagtacg tcgatgtctc catgggcttc ggcatcaacc tgttcggtca
      481 caacccgcgg ttcgtcaccg aggccgtccg ccgccagctc gagcgcggca tgcacgtcgg
      541 catccagtcc gacctcgccg gccaggtcgc cgagcgcctc gcggccctca ccgggctcca
      601 gcgcaccacg ttctgcaact ccggcaccga ggcggtgatg accgcggtgc gcatggcccg
      661 cgcggtcacc ggccgcaccc gcgtcgtgca gttctcgggc tcgtaccacg gccactccga
      721 cgtcacgctg gtcgtcgccg gggcgcgcga cggcgccggc gcgccgatgg cagcgggcgt
      781 tccgggccag accgcccggc agtcgctcgt cctgccctac ggcgaggagc ggtcgctgcg
      841 gatgatcgac gagcacctcg gtgagctcgc cgcggtcctc gtggagccgg tgcagagccg
      901 ccgccccgac ctgcagccgg tcgacttcct gcgccgcctg cgcgagatga cccgccgcgc
      961 cggcgtgccg ctcatcttcg acgaggtgct caccggcctg cgcgcgcatc ccaacggcgc
     1021 ccagggcctg ttcgggatcg acgccgacct cgcgacctat ggcaagatcc tgggcggcgg
     1081 catgccgatc ggcgtggtcg ccggtcaccc gcgattcctc gacgccgtcg acggcgggtc
     1141 gtggcgcttc gacgaccgct cggcgccgcg ggccgagatg accttcgtcg ccggcacctt
     1201 ctgcaagcac ccgctggcga tggcggcgtc cctggcggtg ctcgaccacc tgatcgccga
     1261 agggccggcg ctgcaggccg acctcaaccg ccgcaccgcc gagctcgtcg agcgcctgtg
     1321 cgccgtgctc gccgaggaag gcgcaccgat cgccgtccac cggttcgcct cgctgttccg
     1381 gttcgccgcc gcgcagaact cgagctacct gttccagccc ctggagatgg acctgttcta
     1441 ctacggcctc atcgagcgcg gggtctacgt ctgggagggc cgcacctgct ttttgtcgac
     1501 ggctcacacc gacgccgatc tcgaccacct cgtcgaggcc gtgcgcggca gcgtgcgaga
     1561 gctgatcgcg gccggcttct tcgccgaggc cggccgggcc ccggtgcggc gcgcggaggt
     1621 ggccgacgag gtgaccgacg aggtgaccga cgagcggccg ctgaccgagg cacagcagca
     1681 gctcgccacc ctggcgcgga tgaccgccgg cggttcgagc gcctacaacg tggcgaccag
     1741 cctcgagctg cgcggcgcgc tcgacaccgc ggccctcggc gccgcgctcg gcgacgtcgt
     1801 gcgccgccac gaggcgctgc gcaccgtgct caccgcagac ggcgagcgcc agcgcatcct
     1861 gccgtcgctc gagatcccgc tcgagacgga tctcgagacc gtcggcctcg acggcgacgc
     1921 cggcgccgct gccgtcgatg cgtggctcct ggccgagaac gcccggccgt tcgacctcga
     1981 ggccggcccc ctgctgcgcg cgcgcctctt gcgcctcgcg ccggaccgcc acctgctcgc
     2041 ggtcgtcgct caccacctcg tggtcgacgg ctggtcgatg gaggtcgtcg tgcgcgacct
     2101 cttgcagcgc tacggcgcgc gtgtcaccgg cgcaccggcg ccggcgccgc cgtcgatgca
     2161 gtggcgagac tacctcgcct ggcaagcggc ccaggcggcg cagccggcga tggcggcgca
     2221 cgaggcctac tggcacgagc tcctggcc
//
LOCUS       DASVOR010001708         1573 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_485225,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001708 DASVOR010000000
VERSION     DASVOR010001708.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485225"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="VF516_31930"
     CDS             <1..395
                     /locus_tag="VF516_31930"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEX8112398.1"
                     /translation="KGTYGKLGGWLKQAEALRGLKIVTYHKTWVYFCDRFGIQIRGEI
                     EEKPGIPPSQDYLASLIDKIKREGIKVIFIDTIYPTKDGEFLAGKTGAKIVSSPIDLG
                     GAPGTDTYFALIGTLLDRILAASGAAAK"
     gene            406..>1573
                     /locus_tag="VF516_31935"
     CDS             406..>1573
                     /locus_tag="VF516_31935"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEX8112399.1"
                     /translation="MGYLAQLVEINGLPLLSCLAVSGVLVYLGMHVVERKVIFVDLAL
                     AQIAALGVMWAILMGYDHETDTLPVSLYSLAFTALGALVFSITRTRHERVPHEALIGI
                     IYVAASAGGLVLADRFALGTEALKELIAGRVALVTPEIVGKLAVTCAVVAAVFVGLHR
                     RLMRISTDPAGAEAAGMFLRGWDFLFYLLFGVLITQCVSVLGVLPVFAYLVIPAVAAA
                     FLFTSVRARLIFGWVFASAISLVGLETARLSQLDPGPTIACLFAAALVVLGVGLFLRA
                     RRFAPRIIGRVVGFAVLFAVFVAGTLVFHKTAPTDELGTAIGFARTGDPTRQRQALAS
                     FRKFSSAQAHWLPLVLPMLQDPDRVARSEAVGLVGEVRARGAVAALTARLAVETD"
BASE COUNT          197 a          544 c          567 g          265 t
ORIGIN      
        1 ccaagggcac ctacggcaag ctcggcggct ggctcaagca ggcggaggcc ctgcgcgggc
       61 tcaagatcgt cacctaccac aagacctggg tgtacttctg cgatcggttc ggcatccaga
      121 tccgcggcga gatcgaggag aagcccggga tcccgccgtc gcaggactac ctcgcgtcgc
      181 tgatcgacaa gatcaagagg gagggcatca aggtgatctt catcgacacg atctacccga
      241 ccaaggacgg cgagttcctc gccggcaaga ccggcgcgaa gatcgtgtcg agcccgatcg
      301 acctcggcgg cgcgcccggc accgacacct acttcgcgct gatcggcacg ctgctcgatc
      361 ggatcctcgc agcctccggc gccgcggcga agtgacggac gcgccatggg gtacctcgca
      421 cagctcgtcg agatcaacgg cctgccgctg ctgtcctgcc tcgcggtgtc gggcgtgctg
      481 gtgtacctcg gcatgcacgt ggtcgagcgc aaggtgatct tcgtcgatct cgcgctcgcg
      541 cagatcgccg cgctcggcgt gatgtgggcg atcctgatgg gctacgacca cgagaccgac
      601 acgctgccgg tcagcctgta ctcgctcgcg ttcaccgcgc tcggcgcgct ggtgttctcg
      661 atcacgcgga cccgccacga gcgcgtgccg cacgaggcgc tgatcggcat catctacgtc
      721 gcggccagcg cgggcgggct ggtgctcgcc gaccggttcg cgctcggcac cgaggcgctc
      781 aaggagctga tcgccgggcg ggtcgcgctg gtcaccccgg agatcgtcgg caagctcgcg
      841 gtgacctgtg cggtggtcgc ggcggtgttc gtggggctgc accgccggct gatgcggatc
      901 agcaccgatc ccgccggggc cgaggccgcc gggatgttcc tgcgcggctg ggacttcctg
      961 ttctacctgc tgttcggcgt gctgatcacg cagtgcgtgt cggtgctcgg cgtgctgccg
     1021 gtgttcgcct acctggtgat cccggcggtc gccgcggcgt tcctgttcac cagcgtgcgc
     1081 gcccggctga tcttcggctg ggtgttcgcg agcgcgatca gcctggtcgg cctggagacc
     1141 gcgcggctgt cgcagctcga ccccgggccg acgatcgcgt gcctgttcgc ggcggcgctg
     1201 gtcgtgctcg gcgtcgggct gttcctgcgc gcccgtcgct tcgcgccgcg cataatcggc
     1261 cgggtcgtcg ggttcgcggt gctgttcgcg gtgttcgtcg ccggcaccct ggtgttccac
     1321 aagaccgcgc cgaccgacga gctcggcacg gcgatcgggt tcgcgcgcac cggtgatccg
     1381 acccggcagc gccaggcgct cgccagcttc cgcaagttct cgagcgcgca ggcgcactgg
     1441 ctgccgctgg tgctgccgat gctgcaggac ccggaccgcg tggcgcgcag cgaggcggtc
     1501 ggcctggtcg gtgaggtccg cgcgcgcggc gcggtggcgg cgctcaccgc gcggctcgcg
     1561 gtcgagaccg acg
//
LOCUS       DASVOR010001709         3999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_134310,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001709 DASVOR010000000
VERSION     DASVOR010001709.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134310"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VF516_31940"
     CDS             <1..235
                     /locus_tag="VF516_31940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8112400.1"
                     /translation="ITRRILAQAGALGLPVGPVKRVRAGLRPYRAEVRLERAGRIIHN
                     YGHGGAGYTLSRGCAEAVAALVAGVTAGVTTCR"
     gene            complement(219..1622)
                     /locus_tag="VF516_31945"
     CDS             complement(219..1622)
                     /locus_tag="VF516_31945"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112401.1"
                     /translation="MPEEARPRTNTRLRGEPPPGLQFTGEEGRQRIGRYEVIKTIGKG
                     AMGVVYKARDPLLDRVVAVKTIMSPQGQGRRVRSAFLERFQREAKAAAKMQHPAIVTI
                     FDVGVDEDSGAPFMVLEYLPGESLADRLDRVRFPLARGVQIALDLASALAFAHRQRIV
                     HRDVKPANVLHAGDNRWKLADFGIARMPDSDLTQVGIFMGTPGYSPPEAIREGRYTPQ
                     ADVFAWGAVIYELLSGRIPYEGPDTKTTNSYVVAGNAPAPTRHDTSIPEPLASVTMTA
                     LQSSDRARFKDASEAEQALREAWDRCLTQGLVHPAVLAMEEMPHDKVPPRMHEPRGAV
                     ARPVAPIHVSDSGELTAIDEKMLAKPLAIESSKGRVGDDDPTVLSVRDGKADAARGDR
                     PSGKARALEPERTYPDGSGAREAPRTEKDPSKIVPVAVVRNGSGERLRSNLPLVIVVA
                     MVVLAAVVGALFFTGKL"
     gene            complement(1707..2672)
                     /gene="epsC"
                     /locus_tag="VF516_31950"
     CDS             complement(1707..2672)
                     /gene="epsC"
                     /locus_tag="VF516_31950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEX8112402.1"
                     /translation="MSTERRPLPVNGESRSRVDVVADQLLDLYLDPDAREMQHIGSYE
                     LPSEREVGSIVEQCRALLFPGYAGPDVDRGAPSALRDVIRARIIELRLALHRQVYRAL
                     HHKRQQELGRSDLECVDCADRAESITDRFVGRIPELRRQVRLDLHAAYECDPAATGVD
                     EILLCYPGTYAITVYRMAHALLREGAVVIPRIMTELAHRRTGIDIHPGADIGASFFID
                     HGTGVVIGETTLIGERVRIYQGVTLGALTVPQGETRPTPGQRRHPTIEDNVVIYANAT
                     ILGGDTVIGKGAVIGGNAFVTTSVPPGSKVPGSSRTQRQTAELRG"
     gene            complement(2704..3657)
                     /locus_tag="VF516_31955"
     CDS             complement(2704..3657)
                     /locus_tag="VF516_31955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112403.1"
                     /translation="MTAGTRTGPSSGVQMPQLMFASPRALPRADRLHGRVVVLDVAFA
                     ATIGSTVSFDLVTLPFITALGARLAAWIDHHDHEKHAAFAGDPRFVLATKAQHGACPE
                     MVTPELVRQTGPIDCICTHVDLDGLYAAAKWILGGKQPYPGADDDARAVDTRVGTPGP
                     IAVRIDRALRARSRDDQLKRSVVQFLVGGLQPGVHDQVIAEAAAEFERRDQATRELAQ
                     RFTIRGRVAVVDTAGSQGAFDKTDLLLAGQARAPVSIVRDSGMLTIAAAFDSGWDFVE
                     LFGIGGGMPTRVTIPEQRLDDVIARINEAPAPVPRAAQSDE"
     gene            complement(3680..>3999)
                     /locus_tag="VF516_31960"
     CDS             complement(3680..>3999)
                     /locus_tag="VF516_31960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112404.1"
                     /translation="AADPTRYRVAEDEAPEAIPTTQPPRPRARATFYVDNTRCREPVE
                     VWIDGAQLGQVAAGRRSALVADGGERTLCLLAPGAAQCGDRGTVREVYLHDGWSVTMH
                     CLK"
BASE COUNT          531 a         1462 c         1431 g          575 t
ORIGIN      
        1 catcacgcgc cggatcctcg cgcaggccgg cgcactcggc ctgccggtcg gtccggtcaa
       61 gcgggtgcgc gccgggctgc ggccgtaccg cgccgaggtc cggctcgagc gcgccggccg
      121 gatcatccac aactacggtc acggcggcgc gggctacacg ctgagccgcg gctgcgccga
      181 ggcggtcgcc gcgctggtcg ccggggtcac agccggggtc acaacttgcc ggtgaagaac
      241 agcgcaccga ccaccgccgc gagcaccacc atcgccacga cgatgaccag cggcaggttg
      301 gagcgcagcc gctcgccgga cccgttgcgg accaccgcga ccggcacgat cttcgacgga
      361 tccttctcgg tcctgggcgc ctcgcgcgcc cccgagccgt ccgggtaggt ccgctcgggc
      421 tcgagcgcac gggccttgcc cgacggccga tcgccgcgcg ccgcgtcggc cttgccgtcg
      481 cggaccgaca gcacggtcgg atcgtcgtcg ccgacccgcc ccttgctgct ctcgatcgcg
      541 agcggcttgg cgagcatctt ctcgtcgatc gccgtcagct cgccggagtc gctgacgtgg
      601 atcggcgcca ccggacgcgc gacggcgccg cgcggctcgt gcatccgcgg cggcaccttg
      661 tcgtgcggca tctcctccat cgcgagcacc gcggggtgga ccaggccctg ggtcaggcag
      721 cggtcccagg cctcgcgcag cgcctgctcg gcctcggacg cgtccttgaa ccgcgcgcgg
      781 tccgacgact gcagcgcggt catcgtcacg ctggcgagcg gctcggggat cgaggtgtcg
      841 tggcgggtcg gcgccggcgc gttgcccgcc accacgtagc tgttggtggt cttggtgtcg
      901 gggccctcgt acgggatccg gcccgacagc agctcgtaga tcaccgcgcc ccacgcgaac
      961 acgtcggcct gcggcgtgta gcggccctcg cggatcgcct cgggcggcga gtagcccggc
     1021 gtccccatga agatcccgac ctgggtcaga tcggagtccg gcatccgcgc gatgccgaaa
     1081 tcggcgagct tccagcggtt gtccccggcg tgcagcacgt tggccggctt gacgtcgcgg
     1141 tggacgatgc gctggcggtg cgcgaacgcc agcgccgacg ccaggtcgag cgcgatctgc
     1201 acgccgcgcg cgagcgggaa ccgcacgcga tcgagccggt cggcgagcga ctcgcccggc
     1261 aggtactcga gcaccatgaa cggcgcgccg ctgtcctcgt cgaccccgac gtcgaagatc
     1321 gtgacgatcg ccgggtgctg catcttggcc gcggccttgg cctcgcgctg gaaccgctcg
     1381 aggaacgccg agcgcacccg ccggccctgg ccctgcggcg acatgatggt cttgaccgcg
     1441 acgacgcgat cgagcagcgg atcgcgcgcc ttgtagacca cgcccatcgc gcccttgccg
     1501 atcgtcttga tcacctcgta gcggccgatc cgctgccggc cctcttcccc ggtgaactgc
     1561 agcccgggcg ggggctctcc gcgcagtcgc gtattggttc gcgggcgcgc ttcttcgggc
     1621 atcgcggccg aaaaactcta acacgaccgc ggcgataaca ggcaaatgac agcacgggcc
     1681 cccggctgcg cgcgctgcaa gcccggctag ccccggagct cggcggtctg gcgctgggtc
     1741 cggctcgacc cgggcacctt cgaccccggc gggaccgagg tggtcacgaa cgcatttccg
     1801 ccgatcaccg cgcctttacc gatcacggtg tcacccccga ggatggtcgc gttggcatag
     1861 atcaccacgt tgtcctcgat cgtgggatgt ctgcgctgcc cgggggttgg gcgggtctcg
     1921 ccctgcggga cggtcaaggc gcccagggtc acgccctgat agatccggac ccgctcgccg
     1981 atcagcgtgg tctccccgat caccacgccg gtgccgtggt cgatgaagaa cgaggctccg
     2041 atgtcggccc cggggtggat atcgatcccg gtccggcggt gggccagctc ggtcatgatg
     2101 cgcgggatca ccaccgcgcc ctcccgcagc agggcgtggg ccatccggta caccgtgatc
     2161 gcataggtcc ccgggtagca cagcaggatc tcgtcgaccc cggtcgccgc cggatcgcac
     2221 tcgtaggccg cgtggaggtc gagccggacc tgccggcgca gctcggggat gcggccgacg
     2281 aagcggtcgg tgatcgactc ggcccggtcc gcgcagtcga cgcactcgag gtccgagcgg
     2341 ccgagctcct gctggcgctt gtggtgcagc gcacgataga cttggcggtg cagcgcgagc
     2401 cggagctcga tgatccgggc gcggatcacg tcgcgcagcg cgctcggcgc cccgcggtcg
     2461 acgtcgggcc cggcgtagcc cgggaacagc agcgcgcgac actgctcgac gatcgacccg
     2521 acctcgcgct cgctcggcag ctcgtagctg ccgatgtgct gcatctcgcg cgcgtcggga
     2581 tcgaggtaga ggtcgagcag ctgatccgcg acgacatcga cccgcgatcg cgactcaccg
     2641 ttgactggaa gcggccgccg ttccgtggac atagaaggtt agcataccgc ccctgcggca
     2701 gctttactcg tcgctttgcg cggcccgggg caccggtgca ggtgcctcgt tgatccgggc
     2761 gatcacgtcg tcgagccgct gctccggaat ggtcacccgg gtcggcatgc cgccgccgat
     2821 cccgaacagc tcgacgaagt cccacccgga gtcgaacgcc gcggcgatcg tcagcatgcc
     2881 ggagtcgcgc acgatcgaga ccggcgcccg cgcctggccg gcgagcagga ggtcggtctt
     2941 gtcgaacgcg ccctggctgc ccgcggtgtc gaccaccgcg acccggccgc ggatcgtgaa
     3001 ccgctgggcg agctcgcggg tcgcctggtc gcggcgctcg aactcggccg cggcctcggc
     3061 gatcacctgg tcgtggaccc cgggctggag gccgccgacc aggaactgca ccacgctgcg
     3121 cttgagctgg tcgtcgcgcg acctcgcgcg cagcgcgcga tcgatccgga ccgcgatcgg
     3181 ccccggcgtg ccgacccggg tgtcgaccgc gcgcgcgtcg tcgtccgcgc cggggtacgg
     3241 ctgcttgccg ccgaggatcc acttggcggc ggcgtacagc ccgtcgagat cgacgtgggt
     3301 gcagatgcag tcgatcgggc cggtctggcg caccagctcg ggcgtcacca tctcggggca
     3361 cgcgccgtgc tgcgccttgg tcgccagcac gaaccgcgga tcgcccgcga acgccgcgtg
     3421 cttctcgtgg tcgtggtggt cgatccaggc cgcgagccgc gcgccgagcg ccgtgatgaa
     3481 cggcagggtg accagatcga acgacaccgt gctgccgatc gtggcggcga acgcgacgtc
     3541 gagcacgacg acccggccgt gcagccggtc ggcccggggc agcgcgcgcg gcgaggcgaa
     3601 catcagctgc ggcatctgca cgcccgacga cggcccggtt cgggtacctg cggtcacgat
     3661 ggtcctgcgg cttcggggct cacttcagac aatgcatcgt aaccgaccag ccgtcgtgca
     3721 gatagacctc gcgcaccgtc ccgcggtcgc cgcactgcgc cgcacccggc gccagcaggc
     3781 acagtgtgcg ctcaccgccg tcggcgacca gcgcgctccg gcgcccggcc gcgacctggc
     3841 cgagctgtgc gccgtcgatc cacacctcga ccggctcgcg gcaccgggtg ttgtcgacgt
     3901 agaacgtggc gcgggcgcgc ggtctgggcg gctgcgtggt cgggatcgcc tcgggcgcct
     3961 cgtcctcggc gacccggtag cgggtcgggt cggcggccg
//
LOCUS       DASVOR010001710         1656 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_193191,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001710 DASVOR010000000
VERSION     DASVOR010001710.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1656
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193191"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            281..1087
                     /locus_tag="VF516_31965"
     CDS             281..1087
                     /locus_tag="VF516_31965"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8112405.1"
                     /translation="MELSQAQVETYHREGFLVIEQVFSPPEVAVLQAAIPEITDPSRG
                     KIGFDKASGMVRLSHGAHLYNETIRRLSLHPKLVRPAQQLLGPELHIYQSRLTIKPAA
                     VGSAQPSGWNWHQDFSTWHRYDGMPESRALVTFTFLDDVTAANAPVLVIPRSHHQGMF
                     PTVRDDTEKPSEGYEAVEVKPELVHELAQRAGVTAMTGPAGTVAFMNCAVVHASGENI
                     APLRRALFSVVFNPVNNRPRHPRSKGEHWISTNLNPIQPLADDCLLTMAS"
     gene            1228..>1656
                     /locus_tag="VF516_31970"
     CDS             1228..>1656
                     /locus_tag="VF516_31970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8112406.1"
                     /translation="MFLIGWVQFIVMLDSAIALPLGPDFAKALGIPTSKLGLVSASYT
                     AAVAVVGIAGAFFLDRFDRRRALAVSLIGLVVGTAACGFATGFGSLLAARMVAGVFGG
                     TADTFSFAIVSDAITAERRGRAIGAIMSSLAVASVLGVPAG"
BASE COUNT          268 a          568 c          521 g          299 t
ORIGIN      
        1 gtgccgggct tctggctgtt ctcgcccgag tacctgcgcg gcgtcgcgca gcgcttcggc
       61 tacacggtcc tcgaagcgta cagctttcct tacgagcggc cgctgagcgg cctgcgcaac
      121 cggacgatct gcgtcgcgcg cctggcgggc ggcgacacgg cggcggcgtg acgagatgcc
      181 atcttcttcg aaattacgcg atttcctgat taaccttcaa atctgattcg cgacttgtat
      241 gaaccataca tcgcgtgaga cttccgcaag gggaagcaac atggagttga gccaagcaca
      301 ggtcgaaacc tatcaccgag aggggtttct cgtcatcgag caagtgttct ccccgcccga
      361 ggtggcggtg ctccaggcgg cgatccccga gatcaccgat ccatcgcgcg gcaagatcgg
      421 tttcgacaag gcgagcggga tggtccgcct gtcgcacggc gcgcatctct acaacgagac
      481 gatccgccgg ctgtccttgc atcccaagct ggtgcggcca gcgcaacaac tgctcggacc
      541 cgagctccac atctatcagt cccggttgac gatcaagccc gccgctgtgg gttccgccca
      601 accgagcggc tggaactggc accaggattt ctccacctgg caccgctacg acgggatgcc
      661 ggagtcgcgc gcccttgtca cgttcacctt cctggatgat gtgaccgccg ccaacgcgcc
      721 ggtgctggtg atccctcgct cgcatcacca ggggatgttc cccaccgtca gggatgatac
      781 ggaaaagccc agcgaggggt acgaggccgt ggaggtaaag ccagagctcg tgcacgaatt
      841 ggcccagcga gccggcgtca ccgcgatgac cggtccggcc gggaccgtgg ccttcatgaa
      901 ctgcgccgtg gttcacgcct cgggcgagaa catcgcgccg ctgcgccgcg cgctgttctc
      961 ggtggtgttc aacccggtca acaatcgccc acggcacccc cgctccaagg gggagcactg
     1021 gatctccacg aacctaaacc ccattcaacc gctggccgac gactgcctcc tcacgatggc
     1081 gagctgacgc tcgcttcccg ctcggagact tccgcccgac tgcgtcctca cgacggcgag
     1141 ctgacgctcg cttcccgccg ctgatctgcg actcggcgcg acacccctct ccacgatatg
     1201 cggcccccaa tatccgagcg caagctggtg ttcctgatcg gatgggtgca gttcatcgtc
     1261 atgctcgact cagcgatcgc gctgccgctg ggccccgact tcgcgaaggc gctcgggatc
     1321 cccacctcga agctcggcct ggtcagtgcc agctacaccg cagccgtggc cgtggtcggg
     1381 atcgcgggcg cgttcttcct cgatcgcttc gatcggcggc gagcgctcgc cgtctcactc
     1441 atcgggctcg tcgtcgggac ggccgcctgt gggttcgcga ccggtttcgg cagcttgctc
     1501 gcggcgcgca tggtcgcagg agtgttcggc ggcaccgcgg acacgttctc gttcgcgatc
     1561 gtgagcgatg cgatcacggc cgagcggcgc ggccgagcga taggagccat catgagctcg
     1621 ctcgcagtgg cgtcggtgct cggcgtgccg gctggc
//
LOCUS       DASVOR010001711         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_193197,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001711 DASVOR010000000
VERSION     DASVOR010001711.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193197"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF516_31975"
     CDS             complement(<1..630)
                     /locus_tag="VF516_31975"
                     /inference="COORDINATES: protein motif:HMM:NF012593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEX8112407.1"
                     /translation="MTDTPAPERRFRFTLTHGIVLGLVIGGLLGWLFPEFAVSLKWIS
                     TLFLRLIKMLLAPLLLTTLVVGIAGAGGKMVGRLGLKAILWFEAATTVALFIGLAAAN
                     LVRPGGGVTLAAAAAPASTLAKTKTTTDFVLDAFPASFIDAMAKNDVLQIVVFSVFLG
                     LAVAAAGARAKLIKDVAEAGAEAMFKMVGFVMKFAPFGVGAAIAATLGTS"
     gene            complement(675..1223)
                     /locus_tag="VF516_31980"
     CDS             complement(675..1223)
                     /locus_tag="VF516_31980"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase-like domain-containing protein"
                     /protein_id="HEX8112408.1"
                     /translation="MRIEDYALLGDTQTIALVGRTGSVDWLCFPRFDSGACFAALLGT
                     PEHGRWLIAPRGELRRVERRYRDELVRDGFVHRYRTGPGPNLDGLPGGEGAFLACSFW
                     LVDALVLAGRGDEAGALFDRLLAVRNDVGLLSEEYDTGRRRLVGNFPQAFSDVALVNS
                     ARNRSSPPGPAHHRAQRCGRAP"
BASE COUNT          237 a          596 c          582 g          195 t
ORIGIN      
        1 gctggtgccg agcgtcgccg cgatcgcggc gccgaccccg aacggcgcga acttcatcac
       61 gaacccgacc atcttgaaca tcgcctcggc gccggcctcg gcgacgtcct tgatcagctt
      121 cgcgcgcgcg ccggcggccg ccaccgcgag cccgaggaac acgctgaaca cgacgatctg
      181 gagcacgtcg ttcttcgcca tcgcgtcgat gaacgacgcc gggaacgcgt cgagcacgaa
      241 gtcggtcgtg gtcttggtct tggcgagcgt gctcgccggc gcggccgcgg ccgccagcgt
      301 cacgccgccg cccgggcgga ccaggttggc cgccgccagg ccgatgaaca gcgcgacggt
      361 ggtcgccgcc tcgaaccaga ggatcgcctt gagcccgagc cggccgacca tcttgccgcc
      421 ggcgccggcg atgccgacga ccagcgtggt gagcaagagc ggcgccagca acatcttgat
      481 cagccgcagg aacagcgtgc tgatccactt gagcgacacc gcgaactcgg ggaacagcca
      541 gccgagcagg ccgccgatca cgaggccgag cacgatcccg tgggtgagcg tgaagcgaaa
      601 acgtcgttcc ggcgctggcg tgtccgtcat gcggcctcca cggcgtgcat gttccgccgc
      661 gaccgcgtcc gacttcaagg ggctcggccg caacgctgcg cccggtggtg cgccgggccg
      721 ggcgggctgg accggttgcg cgcgctgttg accagcgcga cgtccgagaa cgcctgcggg
      781 aagttaccga ccagccggcg gcggccggtg tcgtactcct cggacagcag gccgacgtcg
      841 ttgcgcacgg cgagcaggcg gtcgaacagc gcgccggcct cgtcgccgcg gcccgccagg
      901 accagcgcgt cgaccagcca gaacgagcac gccaggaacg cgccctcgcc gccgggcagg
      961 ccgtcgaggt tgggccccgg gccggtgcgg tagcggtgca cgaagccatc gcggaccagc
     1021 tcgtcgcgat agcggcgctc gacccggcgg agctcgccgc gcggcgcgat cagccagcgt
     1081 ccgtgctcgg gggttccgag cagggcggcg aagcaggcgc cggagtcgaa gcgcgggaag
     1141 cacagccagt cgaccgatcc ggtgcgtccg accagcgcga tggtctgggt gtctccgagc
     1201 agcgcgtagt cctcgatccg catggcgatg aatcgtgcaa gccccgctcc gcctcgccgc
     1261 cggtccgcag tcgcacgctg ggacggtcga acacccggcc gtcggcgagc gaccgacaga
     1321 gcttgacgta ctcggcgtgg gcccagccga gcggcgccgc cgagccggtc ggcccgcccc
     1381 ggtacagccc gcggtccggc agatccggcc cgtcccacac ctgctccggc agcatgccgg
     1441 tctcggaggc gcagtgctcc atcatgcgga gcagccggcc ggcggcggtg cgatcgccgc
     1501 gcgcgagctc gtagtgcgcc cgctcgccga tcagcagcgg ccacggccgg ccgaccccgt
     1561 ggccgtcgaa cggcgcgccg tcctcgtgct cgccgtactc gtcgccgggg
//
LOCUS       DASVOR010001712         4734 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_455714,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001712 DASVOR010000000
VERSION     DASVOR010001712.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455714"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /gene="gltD"
                     /locus_tag="VF516_31985"
     CDS             <1..216
                     /gene="gltD"
                     /locus_tag="VF516_31985"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831769.1"
                     /note="glutamate synthase is composed of subunits alpha
                     and beta; beta subunit is a flavin adenine
                     dinucleotide-NADPH dependent oxidoreductase; provides
                     electrons to the alpha subunit, which binds L-glutamine
                     and 2-oxoglutarate and forms L-glutamate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase"
                     /protein_id="HEX8112409.1"
                     /translation="VALDARGNIAVDAGYATSAPGVYCAGDAHRGASLIVWAIAEGRE
                     LARHVDAALRQGRSALPARGQDLPFPA"
     gene            676..1581
                     /locus_tag="VF516_31990"
     CDS             676..1581
                     /locus_tag="VF516_31990"
                     /inference="COORDINATES: protein motif:HMM:NF039441.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6424 family protein"
                     /protein_id="HEX8112410.1"
                     /translation="MTKRNGNGNGHANGNGHGTAKGRVRAQDVQNDHVTKAGEPITYY
                     TAGHPPRSDSPYYIKSRKALHQIIGTHSKPFFGPEPIQDHHGGGLWVYDDEGWFFVRN
                     LVGIEWSGQFCADPAKVDLLRQNARRLYAGFPRSFDEFKSLGIDLKELLDTPITNADG
                     VARWTDSICNASVPLPQPVHTGTVPKGGGVHNYPTPVTDIDFFKRDDFTLWVVDDEGH
                     DVAVVPTHPHGAGKRDLHVIHTEPGSKLNRLHKSAEARGQGFVLQEGDPLHGHLAKQA
                     FAKQSGEGKAKPRRKSGQQAAATPT"
     gene            1788..2687
                     /locus_tag="VF516_31995"
     CDS             1788..2687
                     /locus_tag="VF516_31995"
                     /inference="COORDINATES: protein motif:HMM:NF039441.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6424 family protein"
                     /protein_id="HEX8112411.1"
                     /translation="MAARRNGNGNGHGNGNGNAKRRVRARDVQNVHVSHAGQPITYYT
                     AGHPPRTDSAYYLRSRKALHQIVGTLGQPFFGPEPIQDHHGGGLWVYDDEGWFLVRNL
                     AGIEWSGQFCADPAKVDRLRRNARRLYAGFPRSFDEFKTLGIDLEELLDTPITDAEGI
                     ARWTDSICNASVPLPQPLHTGTVPKGGGVHNYPTPVTDIAFFKRDDFTLWVIDEEGHD
                     VAVVPTHPHSAGQRDLHVIHTEPGSRLNRLHKAVEARGQGFILQEGDPLHAHLARQAF
                     ARQRGTKARSRRKSGPQPAVSPA"
     gene            2800..3414
                     /locus_tag="VF516_32000"
     CDS             2800..3414
                     /locus_tag="VF516_32000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112412.1"
                     /translation="MKALWWSSLLVMIGCAGAPRPAPTPPAAAPPSSCDAHQTAISHG
                     ADTRAAPWSVAQHLAKNFPDGRVSWLMKETSYQSFVVKPAARKWGRCDDTGCYVFVAP
                     AAVIHDAVAHSMTDGHHDPAALGKALGLPAASFDGPLRMMTLDVGKETTCVRLPVDSD
                     PGVWKCQTPQDTDCFQFGGYTSGGVPELMAIDAPVDRTAIEEVQ"
     gene            3526..4503
                     /locus_tag="VF516_32005"
     CDS             3526..4503
                     /locus_tag="VF516_32005"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8112413.1"
                     /translation="MRRSAASMLALLLSATPALADDATGERRADPVVDGAIIAGAVAA
                     SLAPMALPLRDRAIWDSQLLGALDGAVCDQFSQRAAQLSDLALVAAIAAPAAYLTGAT
                     VDDADGDRLVISGEALAVDLALAQVAKYMVHRPRPYLYNHSREAAQYAAAAGDDGWRS
                     FYSSHAAMSFGAAVAGATLLGAASANPYARAAAWTTGFAVATATSNLRVRAGLHFYSD
                     VILGGLIGSAVGYAVPALHAQGKPYAVSRHDVAMAGVGMIAGAVISEALPLGTPGHTD
                     HPPAVMVDQVHVGPLALRDGGGVAISGRVRSRSWGRVPLSHRPADDTAR"
BASE COUNT          748 a         1721 c         1647 g          618 t
ORIGIN      
        1 gtcgcgctcg acgcgcgcgg caacatcgcg gtcgacgccg gctacgccac cagcgctccc
       61 ggcgtgtact gcgccggcga tgcccaccgc ggcgcgtcac tgatcgtctg ggcgatcgcc
      121 gagggccgcg agctcgcgcg ccatgtcgac gcggcgctcc gccagggccg ctccgccctg
      181 cccgcccgcg gccaggacct gccgttcccg gcgtgaccgg cgggcggggc gagccgcctc
      241 gccgcgtgcc cagcgcggcg tgaagccacg ggaaatcgac gggccgtcat cggcggtaat
      301 cggccgtcat ggcgaacgcc gaccgccgag cgcatcgtgg actgccacga ggcaacgtgc
      361 ggcaaatgcg taccaccaga cctgcagcgg tcgcgggcca tcgctgccga tcgcctacgc
      421 ctcgtgtcga agttcgatct ttctggatat cacatcgggt gcggcgctgt acagccggat
      481 cgaaatggaa cctcgtaggt aacaaatttc agtgcagtgg tatcgagtgt cgccgcgcgg
      541 caaaccttgc gcgggcgagc ctgtcgaggc gtgcgccggc ggcgcctgat gaagatttgc
      601 ctgcgacgga tgacgggtat tgccagtgat agccggcagc actattgtcg ctggtcggtg
      661 gaacaaggga agaccatgac caaacggaac gggaacggga acggacacgc gaacgggaac
      721 ggacacggca cggcaaaggg ccgtgtcagg gcgcaggacg tgcagaacga ccacgtcacc
      781 aaggcgggcg agcccatcac gtattatacc gccggccacc caccccgcag cgacagcccg
      841 tactacatca agtcgcgcaa ggcgctgcat cagatcatcg gcacgcacag caagccgttc
      901 ttcggcccgg agccgatcca ggatcaccac ggcggcggcc tgtgggtgta cgacgacgag
      961 ggctggttct tcgtccgcaa cctggtcggg atcgagtgga gcgggcagtt ctgcgccgac
     1021 cctgccaagg tcgatctgct gcgccagaac gcgcggcggc tctacgctgg attccccagg
     1081 tcgttcgacg agttcaagtc gctggggatc gatctcaagg agctgctcga caccccgatc
     1141 accaacgccg acggcgtcgc gcggtggacc gactcgatct gcaacgcgag cgtcccgctg
     1201 ccgcagccgg tccacaccgg gacggtgccc aagggaggcg gtgtacacaa ttatcccacc
     1261 ccggtgaccg acatcgattt cttcaagcgc gacgatttca cgctgtgggt cgtcgacgac
     1321 gagggccacg acgtcgcggt ggtgccgacc cacccgcacg gcgcgggcaa gcgcgatctg
     1381 cacgtcatcc ataccgagcc cggctccaag ctgaaccggt tgcacaagtc ggccgaggcg
     1441 cgaggtcaag ggttcgtgct ccaggaaggc gatccgctcc acggccacct cgccaagcag
     1501 gcgttcgcga agcagagcgg cgaggggaag gccaagcccc ggcgcaagtc cggccagcag
     1561 gccgccgcga ccccgacgta gcgcggcgct cgaccgcccg accgacggct gcgcgcggct
     1621 cgggctggcc atcgctgccg attccccacc tggcaaaacg ctgccgagga tccgtgtcga
     1681 cggcgctcac cgagcgatac gcgctcgcgc tgccgtgccg cgaatgacca cattgccagt
     1741 gacgatcgac cgtcctattg tggtgtgtcg gtcgaacagg cgtgaccatg gcggcgagac
     1801 ggaacgggaa cgggaacggg catggaaacg ggaacggcaa tgcgaagcgc cgtgtccggg
     1861 cccgggacgt ccagaacgtg cacgtcagcc atgcgggcca gcccatcacc tactacaccg
     1921 ccggtcaccc gccgcgcacc gacagcgcgt actacctcag gtcgcgcaag gcgctgcacc
     1981 agatcgtcgg gacgctgggc cagccgttct tcggccccga gccgatccag gatcaccacg
     2041 gcggcggcct gtgggtgtat gacgacgagg gctggttcct cgttcgcaac ctggccggga
     2101 tcgagtggag cggccagttc tgcgccgacc ccgccaaggt cgatcgactg cgccggaacg
     2161 cccggcggct gtacgccgga ttccccaggt cgttcgacga gttcaagacg ctggggatcg
     2221 atctcgagga gctgctcgac accccgatca ccgacgccga gggcatcgcg cggtggaccg
     2281 actcgatctg caacgcgagc gtcccgctgc cgcagccgct ccacaccggg acggtgccca
     2341 agggcggcgg cgtgcacaac tatcccacgc cggtcaccga catcgcgttc ttcaagcgcg
     2401 acgacttcac gctgtgggtc atcgacgagg agggccacga cgtcgcggtg gtgccgaccc
     2461 acccgcacag cgcgggccag cgcgatctgc acgtcatcca caccgagccc ggctcccggc
     2521 tgaaccggtt gcacaaggcg gtcgaggcgc gaggtcaggg gttcatcctc caggaaggcg
     2581 atccgctcca cgcccacctc gccaggcagg cgttcgcgcg gcagcgcggt acgaaggccc
     2641 gctcccgacg gaagtccggc ccgcagcccg ccgtgagccc ggcgtagcga gcggcccggc
     2701 gccgccgcgg aaccatcacc gacgatcgcg agcgatcgat ctgcgttgga tcggctgcgg
     2761 gtgctgcatc ggcggcagcg tcccggatac gctcgacgcg tgaaagccct gtggtggtcg
     2821 agcctgctcg tgatgatcgg ctgcgccggt gcaccccggc cggccccgac gccgccggcc
     2881 gcggccccac cgtcgagctg cgatgcgcac cagaccgcga tcagccacgg cgcggacacc
     2941 cgcgccgcgc cgtggagcgt cgcacagcac ctggcgaaga acttccccga cggccgcgtg
     3001 tcgtggctga tgaaggagac cagctaccag agcttcgtgg tcaagcccgc ggccaggaag
     3061 tggggccggt gcgacgacac cggctgctac gtgttcgtcg cgcccgccgc ggtgatccac
     3121 gacgcggtcg cgcactcgat gaccgacggc caccacgacc ccgccgcgct cggcaaggcg
     3181 ctcggcctgc cggcggcgag cttcgacggc ccgctgcgga tgatgacgct cgacgtcggc
     3241 aaggagacga cctgcgtccg gctgccggtc gacagcgatc ccggcgtgtg gaagtgccag
     3301 acgccccagg acaccgactg cttccagttc ggcggctaca cctccggcgg ggtccccgag
     3361 ctgatggcga tcgacgcgcc ggtcgaccgg accgcgatcg aggaggtcca gtagccggcc
     3421 gccgctcccg tgatctcgcg cgcggcaccg ggcaacccct ggtagcgcgc gtgatcttcg
     3481 cgtgaccgcc ggcggttgcc gccgcgcgcc cgctacggtt gcgcgatgcg ccgatccgcc
     3541 gcctccatgc tcgcgctgct gctgtccgcc acccccgcgc tcgccgacga cgccaccggg
     3601 gagcgccgcg ccgatccggt ggtcgacggc gcgatcatcg ccggcgcggt cgcggcgtcg
     3661 ctggcgccga tggcgctgcc gctgcgcgat cgcgcgatat gggacagcca gctgctcggc
     3721 gcgctcgacg gcgcggtgtg cgaccagttc tcgcagcgcg ccgcgcagct atcggatctg
     3781 gcgctcgtcg ccgcgatcgc cgcgcccgcc gcctacctga ccggcgcgac ggtcgacgac
     3841 gccgatggcg accggctcgt gatctccggc gaggcgctcg cggtcgacct cgcgctggcc
     3901 caggtcgcca agtacatggt gcaccggccc cgcccgtacc tctacaacca cagccgcgag
     3961 gcggcgcagt acgcggcggc ggccggcgac gatggctggc gctcgttcta ctccagccac
     4021 gcggcgatga gcttcggcgc ggcggtcgcc ggggcgaccc tgctcggcgc ggcgagcgcc
     4081 aacccctacg cgcgcgccgc cgcgtggacc accgggttcg cggtggcgac cgcgacctcg
     4141 aacctgcgcg tgcgcgccgg gctgcacttc tactccgacg tcatcctcgg cggcctgatc
     4201 ggcagcgccg tcggctacgc ggtgccggcg ctccatgcgc agggcaagcc gtacgcggtg
     4261 tcgcgccacg acgtcgccat ggccggggtc gggatgatcg ccggcgcggt gatcagcgag
     4321 gcgctcccgc tgggcacccc cggccacacc gatcaccctc cggcggtgat ggtcgaccag
     4381 gtccacgtcg gcccgctcgc gctgcgcgac ggcggcggcg tggcgatcag cggccgcgtc
     4441 cgctcgcgca gctggggccg cgtccccctg tcgcaccggc cggccgacga caccgcccgc
     4501 tgatcgccgg caacgccccc gcagcgatct gctgcggcgg caagatggcg tgcaccggcc
     4561 gccagccgac ggccctgcta ccatcgccgc gcgatggcgt ccaagcgcga ggtggagatc
     4621 gcggacacgc tgccgccgga ggaggacgag ccggcggaca gcggcgagcc ggcgaagccg
     4681 cgcgagacgg tgaagcacga cgaatcggtc gatccggacg aatcggcgaa gcag
//
LOCUS       DASVOR010001713         3289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_426000,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001713 DASVOR010000000
VERSION     DASVOR010001713.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3289
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426000"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2850
                     /locus_tag="VF516_32010"
     CDS             <1..2850
                     /locus_tag="VF516_32010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8112414.1"
                     /translation="GGQAPAAAAPALSDEELAKLAEQEAKTEVITVTGSLIGRKEVDS
                     PSPVSVVDKEKLQSAGITNVGDVLQKIPAQGNAINAQNNNGGDGSTRINLRSLGTART
                     LVLLNGRRVVASGLGADDSVDFGTIPLAMIERVEVLKDGASAIYGSDAIAGVVNVITR
                     GNMNGTEANIYTSTSNRADGTSYDLSFVTGHSSDRGNITFSGGYQNQKPVMASDRNFS
                     KQTYVYDFSCTAAMQATGECVQATFTGSPSSPSGRINTQPNSGSPLMIPGCNTQFCTA
                     DGNGGFRNFIQPSGANLGDNYNFQTLNYLLTPSTRINLFSNGHYDITKNVHGFFEGQF
                     NSRKSTQQLAEEPISTSLFDTPISKDNIYNPFGQDVVDYNRRLTEFGVRAANQEVNTT
                     RLVVGLTGNVSEDVPALKNWKWETSYNYGRTDGTNSLRGDLILSHLANALGPSFRDAQ
                     GAHCGTSGSVIDGCVPLDLLHPRSGNITPDAINYLTFTGTTSGFNEEHTALATASGKL
                     IDLPNHGDISLALGGDYRFERAGFQPDPLTATGDTTGVATEPTLGAYHTFEGFGELSI
                     VPFSGGDTLKWVEVNAAGRAYHYNTFGSGATGKVSGLVRTVGGLALRGTYGTAFRAPN
                     VSELFAGQADTFPNIADPCDAQPPGQKAPGPLDPMIQKVCSGNTPAGGVPVGSVFGSI
                     QQRAKVGGNPDLQPEKGTVGTAGVVYEPLAGLALTLDYWHINIDNAISGLEVSTILAQ
                     CYEGGIDKFCNLVVRDPMSHVISHIFDLNQNVGALQTSGLDFSASYQYRLAAAGTFRH
                     SVEGTYLLKNNIDTGSVDPMTGKEQVLHGRGFFDLGVNPDLKFNVFTTWHHPSGVGAG
                     FAVRFIDSFQECNNNNCNDPSNERRQVSKYADGDVFLDYGLKTSQGVTQIAAGVNNVA
                     NTSPPLIYNGPVLNTSESAYSFMGRQFYVRLSQLF"
     gene            complement(2895..>3289)
                     /locus_tag="VF516_32015"
     CDS             complement(2895..>3289)
                     /locus_tag="VF516_32015"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8112415.1"
                     /translation="NAIEKMTEFNISHPTTFAQWGGVAALDHGDDYVDGLVRRYAEAA
                     ELVYTRLSPLPRVRLARPRSAFYAFFQVDGVTDSFAYAVELMQRARVGLAPGAAFGPS
                     GEGRLRLCFAASLPKLSLALDRLVPALR"
BASE COUNT          593 a         1220 c         1023 g          453 t
ORIGIN      
        1 ggtggccagg ccccggccgc tgcagcacca gcgctcagtg atgaggagct ggccaagcta
       61 gccgagcaag aggccaagac cgaggtgatc accgtcaccg gttcgctgat cggccgcaag
      121 gaggtcgatt cgccgtcgcc ggtgtccgtg gtcgacaagg agaagctgca gagcgccggc
      181 atcaccaacg tcggcgacgt gctgcagaag atccccgccc agggcaacgc gatcaacgcc
      241 cagaacaaca acggcggcga tggctcgacc cgcatcaacc tgcgcagcct cggcaccgcc
      301 cgcaccctgg tgctgctcaa cgggcgccgc gtggtggcga gcggcctggg cgccgatgac
      361 tcggtcgact tcggcacgat cccgctggcg atgatcgagc gggtcgaggt gctcaaggat
      421 ggcgcctcgg cgatctacgg ctcggacgcg atcgccggcg tggtcaacgt gatcacgcgc
      481 ggcaacatga acggcaccga ggccaacatc tacacctcga cgtcgaaccg ggccgacggc
      541 accagctacg acctcagctt cgtgaccggc cactcgtcgg accgcggcaa catcacgttc
      601 tccggcggct accagaacca gaagccggtg atggccagcg accgcaactt ctccaagcag
      661 acctatgtct acgacttcag ctgcaccgcg gcgatgcagg ccaccgggga gtgcgtccag
      721 gccacgttca ccggctcgcc gtcgtcgccg agcggccgga tcaacacgca gcccaacagc
      781 ggctcgccgc tcatgatccc gggttgcaac acgcagttct gcaccgcgga cggcaacggc
      841 gggttccgca acttcatcca gccgtccggc gccaacctcg gcgacaacta caacttccag
      901 accctgaact acctgctgac gccgtcgacc cgcatcaacc tgttctcgaa cggccactac
      961 gacatcacca agaacgtcca cgggttcttc gagggccagt tcaacagccg caagtcgacc
     1021 cagcagctcg ccgaggagcc gatcagcacc agcctgttcg acaccccgat ctccaaggac
     1081 aacatctaca acccgttcgg tcaggacgtg gtcgactaca accgccggct cacggagttc
     1141 ggcgtgcgcg ccgccaacca ggaagtcaac accacgcgcc tggtggtcgg cctcaccggc
     1201 aacgtctccg aggacgtgcc ggcgctcaag aactggaagt gggagacctc gtacaactac
     1261 ggccggaccg acggcacgaa ctcgctgcgc ggcgacctga tcctcagcca cctggccaac
     1321 gcgctcggcc cgagcttccg cgacgcccag ggcgcgcact gcggaacgtc gggcagcgtg
     1381 atcgacggct gcgtcccgct cgacctcctg cacccgcgct cgggcaacat cacgcccgac
     1441 gcgatcaact acctgacgtt caccggcacg acgtccggct tcaacgagga gcacaccgcg
     1501 ctggccaccg cgagcggcaa gctgatcgat ctgcccaacc acggcgacat ctcgctcgcc
     1561 ctcggcggcg actaccggtt cgagcgcgcc gggttccagc ccgacccgct gaccgcgacc
     1621 ggcgacacca cgggcgtcgc cacggagccg accctgggcg cataccatac gttcgagggc
     1681 ttcggcgagc tctcgatcgt gccgttctcc ggcggcgaca cgctcaagtg ggtggaggtc
     1741 aacgccgcgg ggcgcgccta ccactacaac accttcggct ccggggcgac cggcaaggtc
     1801 agcggcctgg tccgcaccgt cggcgggctc gccctccgcg gcacctacgg caccgcgttc
     1861 cgcgcgccga acgtcagcga gctgttcgcc ggccaggccg acacgttccc gaacatcgcc
     1921 gacccgtgcg acgcgcagcc gcccggtcag aaggcgccag gcccgctcga tccgatgatc
     1981 cagaaggtgt gctcgggcaa cacgccggcc ggcggcgttc cggtgggctc ggtgttcggc
     2041 tcgatccagc agcgcgccaa ggtcggcggc aatccggacc tgcagcccga gaaaggcacg
     2101 gtcggcaccg cgggcgtcgt gtacgagccg ctggccggcc tcgccctcac gctcgactac
     2161 tggcacatca acatcgacaa cgcgatctcc ggcctcgagg tgtcgaccat cctggcgcag
     2221 tgctacgagg gcgggatcga caagttctgc aacctcgtcg tgcgcgatcc gatgagccac
     2281 gtgatcagcc acatcttcga cctgaaccag aacgtcggcg cgctccagac ctccggcctc
     2341 gatttctcgg cgtcctacca gtaccggctc gcggcggccg gcacgttccg ccactcggtc
     2401 gagggcacgt acctgctcaa gaacaacatc gacaccggca gcgtcgatcc gatgaccggc
     2461 aaggagcagg tcctgcacgg ccgcgggttc ttcgacctcg gcgtcaaccc cgatctgaag
     2521 ttcaacgtgt tcacgacctg gcaccacccg tcgggcgtcg gcgctggatt cgccgttcgg
     2581 ttcatcgact cgttccagga gtgcaacaac aacaactgca acgatccgag caacgagcgc
     2641 cggcaggtct cgaagtacgc cgacggcgac gtgttcctcg actacgggct caagaccagc
     2701 cagggcgtga cccagatcgc ggccggcgtc aacaacgtcg ccaacacctc gccgccgctg
     2761 atctacaacg gcccggtgct caacacctcg gagtcggcct acagcttcat gggccggcag
     2821 ttctacgtcc ggctgtccca gctgttctga tcccccgggg ggccttgccc cccgctcctc
     2881 gctccgctcg tcggtcagcg cagcgccggc accagccggt cgagcgcgag cgacagcttg
     2941 ggcagcgacg ccgcgaagca cagccggagc cggccctcgc ccgacggccc gaacgcggcg
     3001 cccggcgcca gcccgacccg ggcgcgctgc atcagctcga ccgcgtacgc gaagctgtcg
     3061 gtgaccccgt cgacctggaa gaacgcgtag aacgccgagc gcggccgggc gagccgcacc
     3121 cgcggcagcg gcgacagccg ggtgtagacc agctcggcgg cctcggcgta gcgccggacc
     3181 aggccgtcga cgtagtcgtc gccgtgatcg agcgccgcga ccccgcccca ctgcgcgaac
     3241 gtcgtcgggt gcgagatgtt gaactcggtc atcttctcga tcgcgttcg
//
LOCUS       DASVOR010001714         2720 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_338685,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001714 DASVOR010000000
VERSION     DASVOR010001714.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338685"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VF516_32020"
     CDS             <1..275
                     /locus_tag="VF516_32020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112416.1"
                     /translation="LGAGWRGLWLSPQSGKDTSLQGLELARLQLGVDYRLNEDVAIAP
                     VIGGSLNLFVGEDSPMTSKYTEISDKKVNFIGFAGLAGRFDLGGKR"
     gene            complement(294..>2720)
                     /locus_tag="VF516_32025"
     CDS             complement(294..>2720)
                     /locus_tag="VF516_32025"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HEX8112417.1"
                     /translation="LDDRTEHHSPKPAGTSATVETSDDVRVTTPAKVCGMGHRMKRKE
                     DPRFIQGKGRYVDDIKLPNMVYMDIVRSPYAHAKILKIDASAALATPGVLAVITGEDL
                     KKAGNLHWMPTFMSDTQMVLPIEKVVYYAQEVCVVVATDRYIAADAIEKVVVDYEPLE
                     AVVDPHEALTDKVIVRDDKPKKTNHIWHWEAGDKAATDAVFASAATVVEEKIYLPRIH
                     VSSIETCGMVANYDKARGHMQVYMTSQAPHVVRTVFALVAGLPEHMIQVISPDIGGGF
                     GGKVPIYPGYVCCAVASLVLGRPVKWIEDRSENLQADSFARDYHITARLAADKDGKII
                     GLQVHTVSDCGVSDAAANPSRFPAGLYSICTGSYDMKAAHIAVDGVYTNKPPGGVAYR
                     CSFRVTEAVHMIERMVDILAHQLGKDPADFRLQNFIRPDQFPYKSPTGWEYDSGNYKA
                     ALDKAMAMIDYRALRKEQLEKRKKGQLMGIGISSFTEVVGAGPSKDYDILGIKMFDSA
                     EIRIHPTGKAIARFGTKSQGQGHETTYAQIVAEELGLPAADVQVEEGDTDTAPYGLGT
                     YASRSTPTAGAAAAMAARKIKAKARKIAAYLLEVGEDDLEWHVDSWRVKGSPDKKKTI
                     QEIAFAAYTNHPQGMEAGLEATDYYDPPNLTFPFGSYICVVDIDTGTGEIKIRRFVAV
                     DDCGHIINPMIVEGQIEGGLTMGLAPAMYEEIVYDANGQNLSGTFADYLVPTAVESPK
                     WELGKTVTPSPHHPIGAKGVGESPTVGAPPAIANAIVDAMWHLGVRHIDIPVTPQKVW
                     RLLRKHGVTE"
BASE COUNT          375 a          888 c          947 g          510 t
ORIGIN      
        1 gcctcggggc cggctggcgc gggctgtggc tgtcgccgca gtcgggcaag gacacgtcgc
       61 tacagggcct cgagctcgcg cggctgcagc tcggcgtcga ctaccgtctg aacgaggacg
      121 tcgcgatcgc gccggtgatc ggcggaagcc tcaacctgtt cgtcggcgag gactcgccga
      181 tgacgagcaa gtacaccgag atctcggaca agaaggtcaa cttcatcggc ttcgccgggc
      241 tcgccggccg cttcgacctc ggtggtaagc gctagctcgc agtgccccgc tggtcactcg
      301 gtcacgccgt gcttgcgcag caggcgccac accttctgcg gcgtgaccgg gatgtcgatg
      361 tggcggacgc cgaggtgcca catcgcatcg acgatcgcat tggcgatcgc cggcggggcg
      421 ccgacggtgg gcgactcgcc gacgcccttg gcgccgatcg ggtggtgggg cgacggcgtg
      481 accgtcttgc cgagctccca cttcggcgac tcgaccgcgg tcggcacgag gtagtcggcg
      541 aacgtccccg acaggttctg gccgttggcg tcgtagacga tctcctcgta catcgccggc
      601 gcgaggccca tggtcaggcc gccctcgatc tggccctcga cgatcatcgg gttgatgatg
      661 tggccgcagt cgtcgaccgc cacgaaccgc cggatcttga tctcgccggt gccggtgtcg
      721 atatccacca cgcagatgta gctgccgaac gggaacgtca ggttcggcgg gtcgtagtag
      781 tcggtggcct cgaggccggc ctccatgccc tgcgggtggt tggtgtacgc ggcgaacgcg
      841 atctcctgga tcgtcttctt cttgtcgggc gagcccttca cccgccagct gtcgacgtgc
      901 cactcgaggt catcctcgcc gacctcgagc aggtacgcgg cgatcttcct ggccttcgcc
      961 ttgatcttgc gggcggccat cgccgcggcg gcgccggcgg tcggcgtcga ccggctggcg
     1021 taggtgccca gaccgtacgg cgcggtgtcg gtgtcgccct cctcgacctg gacgtcagcg
     1081 gccggcaggc cgagctcctc ggcgacgatc tgggcgtagg tggtctcgtg gccctggccc
     1141 tgcgacttgg tgccgaaccg cgcgatcgcc ttgccggtgg ggtggatgcg gatctcggcc
     1201 gagtcgaaca tcttgatgcc gaggatgtcg tagtccttcg acgggccggc gccgacgacc
     1261 tcggtgaagc tcgagatccc gatgcccatc agctggccct tcttgcgctt ctcgagctgc
     1321 tccttgcgca gcgcgcgata gtcgatcatc gccatcgcct tgtcgagcgc ggccttgtag
     1381 ttgccgctgt cgtactccca gccggtcggc gacttgtacg ggaactgatc ggggcggatg
     1441 aagttctgca gccggaagtc ggcggggtcc ttgccgagct gatgggcgag gatgtcgacc
     1501 atgcgctcga tcatgtggac ggcctcggtg acccggaacg agcagcggta ggcgacgccg
     1561 cccggcggct tgttggtgta gacgccgtcg accgcgatgt gcgccgcctt catgtcgtac
     1621 gatccggtgc agatgctgta caggccggcc gggaacctcg acgggttggc ggcggcgtcg
     1681 gacacgccgc agtccgacac cgtgtggacc tggaggccga tgatcttgcc gtccttgtcg
     1741 gcggcgagcc gcgcggtgat gtggtagtcg cgcgcgaagc tgtcggcctg gaggttctcg
     1801 ctgcgatcct cgatccactt gaccggccgg ccgagcacga ggctggcgac cgcgcagcac
     1861 acgtagccgg gatagatcgg gaccttgccg ccgaacccgc cgccgatgtc gggcgagatg
     1921 acctggatca tgtgctcggg cagccccgcg accagcgcga acaccgtgcg gacgacatgc
     1981 ggcgcctgcg acgtcatgta gacctgcatg tggccgcgcg ccttgtcgta gttggcgacc
     2041 atgccgcagg tctcgatcga tgagacatgg atccgcggca ggtagatctt ctcctcgacc
     2101 acggtcgccg cgctggcgaa caccgcgtcg gtggcggcct tgtcgccggc ctcccagtgc
     2161 cagatgtggt tggtcttctt cggcttgtcg tcgcgcacga tcaccttgtc ggtgagcgcc
     2221 tcgtgcggat cgaccaccgc ctcgagcggc tcgtagtcga cgacgacctt ctcgattgca
     2281 tcggcggcga tgtagcggtc ggtggcgacc accacacaga cctcctgcgc gtaatacacc
     2341 actttctcga tcggcaatac catctgggta tccgacatga aggtcggcat ccagtgcagg
     2401 ttgcccgcct tcttgagatc ctcgccggtg atcacggcga gcacgccggg ggtcgcgagc
     2461 gcggcgctgg cgtcgatctt gaggatcttg gcgtgcgcgt acgggctgcg cacgatgtcc
     2521 atgtagacca tgttgggcag cttgatgtcg tcgacgtagc ggcccttgcc ctggatgaac
     2581 cgcgggtcct ccttgcgctt catccggtgg cccatgccgc acaccttggc cggcgtggtg
     2641 acccggacgt cgtcgctggt ctccaccgtg gccgaggtcc cggccggctt gggcgagtga
     2701 tgctcggtgc ggtcatcgag
//
LOCUS       DASVOR010001715         1834 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_106143,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001715 DASVOR010000000
VERSION     DASVOR010001715.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1834
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106143"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VF516_32030"
     CDS             complement(<1..1199)
                     /locus_tag="VF516_32030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8112418.1"
                     /translation="MTATDRRALLAAERGTLHKQAETRIALVYPSPYRAAMSSLGDQQ
                     IYRVLHAMPGVAADRAMLPEPGPGSDAVRGGAFVTLETARPVGGYPMLAYSVAYELEI
                     AGVVDTLERAGVPVLAADRDDRHPLVIAGGPLTFSNPAPLAPFFDLVVLGEGEQLVPE
                     LVAIAREVGFVRGRVWDALAGRPGYFLPHVHGERVPAVAAADDALLPARSVIATPNTE
                     LADMFLTEAARGCSRGCTYCVMRRSTNGGMRIVARRDIIAGIPDDARRVGLVGAAVTD
                     HPEIAAIVRDVVDAGRQVGISSLRADKLDDELVGLLARGGYRTLTVAADGASERMRRI
                     VERSTQARHLVRSAELAAGYRLQTLKIYMMLGVPGETDADVDELVALSRELAAIHPRV
                     AYGVAPFV"
     gene            complement(1293..>1834)
                     /locus_tag="VF516_32035"
     CDS             complement(1293..>1834)
                     /locus_tag="VF516_32035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112419.1"
                     /translation="AGPSGRVVRVERSGSSRIPPRLCEIHGDGGNCVGDEPRAGQTVA
                     VLDEHHVVAEVQIVEATSFSASCPMLWTVKTRLLRGAPVDSDEIGVIDPSLDTARAHL
                     INRTQLPSSPSGLPDDEVWRAIDRDGDGAADILITRYGCDASGRPTSAGNPQCIDLWA
                     RTGARMTRTTQLNFALCNR"
BASE COUNT          233 a          688 c          684 g          229 t
ORIGIN      
        1 acgaacggcg cgacgccgta ggcgacccgg gggtggatcg ccgccagctc gcgcgacagc
       61 gcgaccagct cgtcgacatc ggcgtcggtc tcgcccggca cgccgagcat catgtagatc
      121 ttcagggtct gcagccgata ccccgcggcg agctcggccg agcgcaccag gtggcgcgcc
      181 tgggtcgagc gctcgacgat ccgccgcatc cgctcgctcg cgccgtcggc ggcgacggtc
      241 agcgtgcggt agccgccgcg ggcgagcagc ccgaccagct cgtcgtcgag cttgtcggcg
      301 cgcaggctgg agatcccgac ctggcggccg gcgtcgacca cgtcgcgtac gatcgcggcg
      361 atctcggggt gatcggtgac cgcggcgccg accaggccga cccgccgcgc gtcgtcgggg
      421 atccccgcga tgatgtcgcg gcgcgcgacg atccgcatgc cgccgttggt cgaccgccgc
      481 atcacgcagt aggtgcagcc gcgcgagcag ccgcgcgcgg cctcggtcag gaacatgtcg
      541 gcgagctcgg tgttcggcgt cgcgatcacc gagcgggcag ggagcagcgc gtcgtcggcc
      601 gcggcgaccg ccggcacccg ctcgccgtgg acgtggggca ggaagtagcc gggccgcccg
      661 gcgagcgcgt cccacacccg cccgcgcacg aagccgacct cgcgcgcgat cgcgaccagc
      721 tcggggacca gctgctcgcc ctcgccgagc accacgaggt cgaagaacgg cgcgagcggc
      781 gccgggttgg agaacgtcag cggtccgccc gcgatcacca gcgggtggcg gtcgtcgcgg
      841 tcggcggcga gcaccggcac gccggcgcgc tccagcgtgt cgaccacccc ggcgatctcc
      901 agctcgtacg cgaccgagta cgcgagcatc gggtagccgc cgaccgggcg cgcggtctcc
      961 agcgtcacga acgccccgcc gcgcaccgcg tcggagcccg gcccgggctc gggcagcatc
     1021 gcgcgatccg cggcgacccc cggcatcgcg tgcagcaccc ggtagatctg ctggtcgccc
     1081 agcgacgaca tcgcggcgcg gtacgggctg ggatacacca gcgcgatccg ggtctcggcc
     1141 tgcttgtgca gcgtgccgcg ctcggcggcg agcagcgcgc gccggtcggt cgcggtcacg
     1201 gccgggcgag cctcgtgggc agggagttct cggagcggga catggcgatc cgcctcatgg
     1261 ctaccacacc gccgcgccgc cgtcgcacgg ggtcagcgat tgcacagcgc gaagttgagc
     1321 tgggtcgtgc gcgtcatccg cgctccggtc cgggcccaca ggtcgatgca ctgcgggtta
     1381 ccggcgctgg tcggccggcc cgacgcgtcg cagccgtagc gggtgatcag gatgtcggcg
     1441 gcgccgtcgc cgtcgcggtc gatcgcccgc cagacctcgt cgtcgggcag cccgctcggg
     1501 ctcgacggca gctgggtgcg gttgatcagg tgcgcccggg cggtgtccag gctcgggtcg
     1561 atcacgccga tctcgtcgct atcgaccggc gcgccgcgca gcagccgggt cttgaccgtc
     1621 cacagcatcg ggcagctcgc cgagaagctg gtcgcctcca cgatctggac ctccgcgacc
     1681 acgtggtgct cgtcgagcac ggccacggtc tgtccggcgc gcggctcgtc gccgacgcag
     1741 ttgccgccgt cgccgtggat ctcgcacagc cgcggcggga tccgcgagct gccggagcgc
     1801 tcgacccgga ccacccggcc gctcggcccg gccg
//
LOCUS       DASVOR010001716         2628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_573701,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001716 DASVOR010000000
VERSION     DASVOR010001716.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573701"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1206
                     /locus_tag="VF516_32040"
     CDS             <1..1206
                     /locus_tag="VF516_32040"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HEX8112420.1"
                     /translation="LDAAPPTARQGPAAELVLAADAFVIRPAGRHEDHIRARIAGDEA
                     WTVIAGYHWFTDWGRDTMISLEGLTLASGRTAEAGSILRMFAHHLRDGLVPNMFPEGA
                     NEGLYHTADATLWFFHAIDRYVRASDDHRTLAHLLPALVDVIDHHMAGTRFNIHVDPR
                     DGLLGQGAEGYPLTWMDAKVNDWVVTPRRGKAVEINALWYNALRLMQSWLDDAGDRPR
                     AARMRHAAERAQAAFNQRFWSPERGYLYDVVDGEHGDDPACRPNQLFAISLPHPVLDP
                     GHWRPVLESVERELVTPVGLRSLSPHHPDYKPTYRGDLMTRDAAYHQGTVWSWLIGPY
                     VDAVLRVRGDAVGARRALDGLIAHLRENCIGYVSEVFDAEPPFTPGGCVAQAWGIAEL
                     LRCLLRTER"
     gene            1294..1569
                     /locus_tag="VF516_32045"
     CDS             1294..1569
                     /locus_tag="VF516_32045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112421.1"
                     /translation="MQHHPNPSAQSENNESEPAEAHGLPWQVEVGRLLTQAAAICVEQ
                     GVDVDAFMKGAWSAYVESRPGMRDYLEEMQLRDQLEEIRKLGRMGQA"
     gene            complement(1636..2148)
                     /locus_tag="VF516_32050"
     CDS             complement(1636..2148)
                     /locus_tag="VF516_32050"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8112422.1"
                     /translation="MPNPTATFETSLGTFTAEIFQDKMPITASNFVKIAKSGFYDGLH
                     FHRVIPGFMVQFGCKYSKDPSSPNCGRGDSPFGRIKDEHPPEHKLSNEPGTLSMANSG
                     PNSGGAQFFINTVHNAYLDWFTPGQSKHPVFGKVTSGLDVVKKIETTPTDQGDRPRTP
                     VKMIKVTVSE"
     gene            complement(2217..>2628)
                     /locus_tag="VF516_32055"
     CDS             complement(2217..>2628)
                     /locus_tag="VF516_32055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112423.1"
                     /translation="DRGFMKLTKEQVAAVVTEASKKMSDPNYSAMLVGGFVESQTPVA
                     QFISAHDRELGGAEAIVNVIFHCALVAQCFQRNGGRVRTLSYEDLDAAARGEPLARLE
                     AAQLPLHEFIKANIEHAEAQKLVAMIALAIHGAS"
BASE COUNT          396 a          929 c          876 g          427 t
ORIGIN      
        1 ctcgacgccg cgccgccgac cgcgcgccag ggccccgccg ccgagctcgt gctcgccgcc
       61 gacgccttcg tcatccgccc cgccggccgc cacgaggacc acatccgcgc gcgcatcgcc
      121 ggcgacgaag cctggaccgt gatcgccggc tatcactggt tcaccgactg gggccgcgac
      181 acgatgatct cgctcgaggg tctgaccctg gcgagcggcc gcaccgccga ggccggatcg
      241 atcctgcgga tgttcgccca ccacctgcgc gacggcctgg tgcccaacat gttccccgag
      301 ggtgccaacg agggcctgta ccacacggcc gacgccacgc tgtggttctt ccacgcgatc
      361 gatcgctacg tgcgcgccag cgacgaccac cgcacgctcg cccacctgct gcccgcgctg
      421 gtcgacgtca tcgatcacca catggcgggc acgcggttca acatccacgt cgacccccgc
      481 gacggcctgc tcggccaggg cgccgagggc tacccgctga cctggatgga cgccaaggtc
      541 aacgactggg tggtcacgcc tcgccgcggc aaggcggtcg agatcaacgc gctctggtac
      601 aacgcgctgc gcctcatgca gtcgtggctc gacgacgccg gcgatcgccc gcgcgcggcg
      661 cgcatgcggc acgccgccga gcgcgcgcag gccgcgttca accagcggtt ctggagcccc
      721 gagcgcggct acctgtacga cgtcgtcgac ggcgagcacg gggacgatcc ggcgtgccgg
      781 cccaaccagc tgttcgcgat ctcgctgccc caccccgtgc tcgaccccgg gcactggcgg
      841 cccgtgctcg agtccgtcga gcgcgagctg gtcacgccgg tcgggctgcg ctcgctgtcg
      901 ccgcaccacc ccgactacaa gccgacctat cggggagatc tgatgacccg tgacgccgcc
      961 taccaccagg gcacggtgtg gagctggctg atcgggccgt acgtcgatgc cgtgctgcgc
     1021 gtgcggggcg acgccgtggg cgcccggcgc gcactcgatg gcctgatcgc tcacctgcgg
     1081 gaaaattgca tcggctacgt gagcgaggtc ttcgacgccg agccgccctt cactccgggt
     1141 ggttgcgtcg cccaggcgtg ggggatcgcc gagctgctgc gctgcctgct gcgcaccgag
     1201 cgctgaacgc tgcccgaccg gtcaggtcca ccctggtcgt tctaggcttg cggcgggcac
     1261 gaacgatgct tttgctatgg acaggagtcc accgtgcagc accatccaaa cccctcggcg
     1321 cagtccgaga acaatgaatc ggaaccggcc gaagctcacg ggctgccatg gcaggtcgag
     1381 gtcggtcggc ttctcaccca ggcggcggcg atctgcgtcg agcaaggcgt ggacgtcgat
     1441 gccttcatga agggcgcgtg gtcggcctac gtcgaatctc gccccggcat gcgcgactac
     1501 ctggaagaga tgcagctccg cgatcagctg gaggagatcc gcaagctggg ccgcatgggc
     1561 caggcctgac ccgatccggc gtgcccgact gcacgcgatg cgcagctgac tcgctgaccg
     1621 tgacggatgc gcagcttatt cgctgaccgt caccttgatc atcttgaccg gcgtcctcgg
     1681 ccgatcgccc tggtcggtcg gcgtggtctc gatcttcttg accacgtcca accccgacgt
     1741 gaccttgccg aacaccgggt gcttggactg acccggcgtg aaccagtcga ggtaggcgtt
     1801 gtggacggtg ttgatgaaga actgcgcgcc gcccgagttg gggccgctgt tggccatcga
     1861 cagcgtaccc ggctcgttcg acagcttgtg ctcgggcggg tgctcgtcct tgatccgacc
     1921 gaacggcgag tcaccgcggc cgcagttcgg gctgctggga tccttggagt acttgcagcc
     1981 gaactggacc atgaagcccg ggatcacccg gtgaaagtgc agcccgtcgt agaacccgct
     2041 cttcgcgatc ttcacgaagt tgctggcggt gatcggcatc ttgtcttgaa agatctcggc
     2101 ggtgaacgta ccgagcgagg tctcgaacgt cgcggtcgga ttcggcatcc cggaggctta
     2161 tcacaactgc gcgaccgacg cgctgtgggt cgcgcaccgc ctggcccgtg cagcggctag
     2221 ctcgcgccgt ggatcgccag cgcgatcatc gcgaccagct tctgggcctc ggcgtgctcg
     2281 atgttcgcct tgatgaactc gtgcagcggg agctgggcgg cctccaggcg ggcgagcggc
     2341 tcgccgcgcg cggcggcgtc gaggtcctcg taggacagcg tgcggacccg gccgccgttg
     2401 cgctggaagc actgcgccac gagcgcgcag tgaaagatca cgttgacgat cgcctccgcg
     2461 ccgccgagct cgcggtcgtg cgcgctgatg aactgcgcga ccggggtctg cgactcgacg
     2521 aacccgccga ccagcatcgc cgagtagttc gggtctgaca tcttcttcga cgcctcggtg
     2581 accaccgcgg cgacctgctc cttggtcagc ttcatgaaac ctcgatcg
//
LOCUS       DASVOR010001717         2183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_279753,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001717 DASVOR010000000
VERSION     DASVOR010001717.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279753"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="VF516_32060"
     CDS             complement(<1..626)
                     /locus_tag="VF516_32060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112424.1"
                     /translation="MTDLSGSYRFLAWARSGLAAAPPAQATGDARLRLSVTLTVRKGD
                     AQTDTVPRALRLYGPGDVIGLDGKQIIRREPRPGTSDFEPNYFPLIEFDAPELPWLFS
                     PEPGDTQLRPWLALVVLRRELAQISTDARRPLPWLSLAPSDARDELPDLSEAWAWAHV
                     QIAGSSLAPAEALDGRNPELTLSRLLSPRRLRPRQAYTACLVPVYRAGV"
     gene            complement(623..>2183)
                     /locus_tag="VF516_32065"
     CDS             complement(623..>2183)
                     /locus_tag="VF516_32065"
                     /inference="COORDINATES: protein motif:HMM:NF042646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6603 domain-containing protein"
                     /protein_id="HEX8112425.1"
                     /translation="PLSHVASLLGTLSAVFPVAENRYVFGPMVRIGWGSPTLITLDLA
                     LILEIPDPVRLIILGRLKAVLPNEKAPVVQLRLDALGVLDFSRNEFSLDATLYDSRIV
                     TFALTGDMALRLSWGSQPAFAMALGGFHPAFKPPPGFPALRRLALALSTGDNPRLRME
                     SYFALTSNTVQVGARLELYVKIAAFSLEGGLGFDTLIQFSPFRLLADIDAHLALKRGS
                     TTLMGLDINVHLMGPAPWVLWGQAKFKIFFVSFSIPFRATFGQSQDVPPIERREVWPI
                     LRDSLSATASWSAQLPGDSGRLVALRAGAGGDDLLVHPLGTLAVSQNLVPLDRTLGLF
                     GAVPPKDFDRFAIIAATGLDITDTTTQYFAPAQFRQMTDAEKLASPSFERMVSGAQFA
                     PHTAIAIGHVQDTPLDYEQAVILDVDQPASERLDQRYAPAGATVAVLAEHGPAGTAAV
                     RTQGRAKFAPPAPGPKVADPSYVIATRNDLTRVDVDGHDGSYTSAAERLRQRADRDAL
                     QIVRAEELELT"
BASE COUNT          318 a          757 c          817 g          291 t
ORIGIN      
        1 acgccggcgc gatagaccgg caccaggcac gccgtgtacg cctgccgcgg tcgtagccgc
       61 cgcggcgaca ggaggcgcga cagcgtcagc tcgggattgc ggccgtcgag cgcctcggcc
      121 ggcgccagcg agctgccggc gatctgcacg tgcgcccagg cccacgcctc ggacagatcg
      181 ggcagctcgt cgcgcgcgtc cgacggcgcc aggctcagcc agggcagcgg cctgcgcgcg
      241 tcggtggaga tctgggcgag ctcgcggcgg agcaccacca gcgcgagcca cggccgcagc
      301 tgcgtgtcgc ccggctcggg gctgaacagc cacggcagct ccggagcgtc gaactcgatc
      361 agcgggaagt agttcggctc gaagtcgctg gtgcccggcc gcggctccct gcggatgatc
      421 tgcttgccgt cgaggccgat cacgtcgccg gggccgtaca gccgcagcgc ccgggggacc
      481 gtgtcggtct gcgcgtcgcc cttgcgcacg gtcagcgtca ccgacaggcg cagccgggcg
      541 tcgccggtgg cctgcgccgg aggtgccgcc gccaggccgc tgcgggccca tgcgaggaag
      601 cgatagctgc cggacaggtc cgtcatgtga gctccagttc ctccgcgcgc acgatctgca
      661 gcgcgtcgcg gtcggctcgc tggcgcagcc gctcggccgc ggacgtgtag ctgccgtcgt
      721 gcccgtcgac gtccacccgc gtcaggtcgt ttcgggtggc gatcacgtac gacgggtcgg
      781 cgaccttcgg gcccggcgcg ggcggcgcga acttcgcccg gccctgggtg cgcaccgcgg
      841 cggttccggc cgggccatgc tcggcgagca ccgcgaccgt cgcgccggcg ggcgcgtagc
      901 gctgatcgag ccgctcggag gccggttgat cgacgtcgag gatgacggcc tgctcgtagt
      961 cgagcggtgt gtcctgcacg tggccgatcg cgatcgcggt gtgcggcgcg aactgcgcac
     1021 cggacaccat ccgctcgaac gacgggctcg cgagcttctc ggcgtcggtc atctgccgga
     1081 actgcgccgg tgcgaagtac tgcgtcgtgg tgtcggtgat gtccagcccg gtggcggcga
     1141 tgatcgcgaa gcggtcgaag tccttcggcg gtaccgcccc gaacaggccc agggtgcgat
     1201 ccagcgggac gaggttctgc gacaccgcca gcgtgccgag cgggtggacg agcagatcgt
     1261 ctccgccggc accggcgcgc agcgccacca gccggccgct gtcgcccggc agctgcgcgc
     1321 tccagctggc cgtggcggag aggctgtcgc gcaggatcgg ccacacctcc cggcgctcga
     1381 tcggcggcac gtcctgcgac tggccgaacg tggcgcggaa cgggatcgag aagctgacga
     1441 agaagatctt gaacttcgcc tgaccccaca gcacccacgg ggcgggaccc atcaggtgca
     1501 cgttgatgtc gaggcccatc agcgtggtgc tgccgcgctt gagcgcgagg tgggcgtcga
     1561 tgtcggccag cagccggaac ggtgagaact ggatcagcgt gtcgaacccg agcccgccct
     1621 cgaggctgaa cgcggcgatc ttgacgtaga gctcgagccg cgcgccgacc tgcaccgtgt
     1681 tggaggtcag cgcgaagtac gactccatgc gcagccgtgg gttgtcgccg gtcgacagcg
     1741 ccagggcgag ccgccgcagc gcggggaacc ccggcggcgg cttgaacgcc gggtggaagc
     1801 cgcccagcgc catggcgaac gcgggctggc tgccccacga cagccgcagc gccatgtcgc
     1861 cggtgagcgc gaacgtgacg atgcgcgagt cgtacagcgt ggcatcgagc gagaactcgt
     1921 tgcggctgaa gtccagcacg ccgagcgcat ccaggcggag ctgcaccacc ggcgccttct
     1981 cgttgggcag cacggccttg agccggccca ggatgatcag gcggacggga tccgggatct
     2041 ccaggatcag ggccaggtcg agcgtgatga gggtgggcga gccccagccg atccggacca
     2101 tcggcccgaa cacgtagcgg ttctccgcca ccggaaacac cgcgctcagc gtgccgagca
     2161 ggctcgccac gtggctcagc gga
//
LOCUS       DASVOR010001718         4896 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_396873,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001718 DASVOR010000000
VERSION     DASVOR010001718.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396873"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VF516_32070"
     CDS             <1..552
                     /locus_tag="VF516_32070"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8112426.1"
                     /translation="RLAEHGMGGVPASEAWAALDGFLHGDAEVVGYVPLDLRRWFDAY
                     PETAAQATWQALRQAALQGNPLGLPAQPGFADEAFRSQLEASPALTRRDLAETKVREL
                     ASRVLRLDVTELDGDTPLKALGLDSLMGLELRNRLESAFGLKLSPTLLWTYGSSRALA
                     GMLCEHVFGAPTSCLPASRHEDP"
     gene            549..3716
                     /locus_tag="VF516_32075"
     CDS             549..3716
                     /locus_tag="VF516_32075"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8112427.1"
                     /translation="MTTAPTTPPTPLSRALETIRRLKAQLASLGAGQPVAIVGVGMRF
                     PGAIDSLESYWQALSEGRDLVRAMPAARKAPFAAEWETLPHKGGFLDEVTRFDAAFFG
                     ISPREARALDPQHRLLLEVAWEALENAALPPDRLTGVRTGLYVGITGQDYRDWQVGAP
                     DAYWATGNGHCFAAGRIAYAMGFTGPAIAVDTACSSGLVAVHLASQAIRRGECDVAVA
                     GGVNLVLSPRSTRLLVETRALAPDGLCKTFDARANGFARGEGCGIVVLKRLDHALRDR
                     DHVHAVIHGSAVNQDGRSSGFTAPNVRAQIALIEAALVDAGLTPADIGLIEAHGTGTA
                     LGDPIEMEALVAALGRNNAGATLYVGSVKPNIGHLEAAAGIAGLLKAVACCAHRAIPA
                     LVHHRTLSPRIDLAGTGIRLPTTLVPWTTDVGRFAGVSSFGLSGTNAHMILGAGDPGG
                     ARPAQGARGFELTARTPEALRALAARFRHRLAALPDDQYGAFAYTATSGRTRHPVRAR
                     IAASDKTAALAALDALAGNMPCPAVRLGAGEEPFEDLPRCVVVLPHYPWQREHHAPQP
                     AVESASVGTARATSAPPASDRLDATRTQVRRHVATVLGHHDASAVPDDASLFDLGLDS
                     MMAVDLACALGSALGVELSLTHVLSHPTVNGLAATIVAGLPVPAPSPARAPVAAASEP
                     AHPAARPTAGPMRAPRVAFLFSCQGSQYFGMGRELYNTEPVFRARIDACDRILAPMLG
                     ASLEALMMHGDDRDAIHHTRVAQPALVALQLALADLWKSWGVTPAVVMGHSLGEVAAA
                     IHAGVMDLVSGLTLVAHRARLMASTPRGAMLAVTAPLAQVRAWLAGTSIDVAAINGPE
                     SIVVSGAHDAIEALTARLHAEGVVVRPLAVPLASHSRVMEPIVRPLHDAIAHLAFHPP
                     TLPILSNLTGQLAAADQYGAEYWSRHLCEPVRFHDGVQALRALDIDVCLEIGPDGTLA
                     NLVMATGLLTGGASVASLRRGASDRASMLGAVDALHARGQQFAWSEVLAVSRAKPSAI
                     APWPLVASISGAMADIRRGDKRGAA"
     gene            3725..4474
                     /locus_tag="VF516_32080"
     CDS             3725..4474
                     /locus_tag="VF516_32080"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8112428.1"
                     /translation="MRPHPLANPSLRVIAFHHAGGSAAMYHPMSAGMPAGWELLLLDL
                     PGRGKRYAEEPLRTTPALVARVVEDVRPWLDIPVALFGHSLGAILAAEVGRACEQLGA
                     PPVWVGVSGRVAPTLQAHALRLNDLDDASLLAEVLDLGGTPDRLIEIPELRTHFLRVV
                     RADIAVLDSYQPAPDRAALGCPMTAFAGTGDVWAPPATMRPWARETCSDFCLRQFEGG
                     HFYFLGPAFARFTRELVHEIEPYADALAGPS"
BASE COUNT          624 a         1820 c         1730 g          722 t
ORIGIN      
        1 cgcctcgccg agcacggcat gggtggggtc ccggcatcgg aggcgtgggc cgcgctcgac
       61 ggcttcctgc acggcgacgc cgaggtggtc ggctacgtcc cgctcgatct gcggcgctgg
      121 ttcgacgcgt accccgagac cgccgcgcag gcgacctggc aggcgctccg ccaggccgcg
      181 ctgcagggca accccctcgg tctgcccgcc cagccggggt tcgccgacga ggcgtttcgc
      241 agccagctcg aggcgagccc cgcgctcacc cggcgggacc tcgccgagac caaggtgcgc
      301 gagctcgcga gccgcgtgct ccgcctcgac gtgaccgagc tcgacggcga cacgccgctc
      361 aaggcgctcg gcctcgactc gttgatgggc ctcgagctcc gcaaccgcct cgagtccgcg
      421 ttcggtctca agctgtctcc cacgctgctg tggacctacg gcagctcgcg cgcgctcgcc
      481 ggcatgctgt gcgagcacgt gttcggcgcg cccacgtcct gcctccctgc ttctcgccac
      541 gaggatccat gaccaccgcc cccaccactc ccccgactcc gctgagccgc gcgctcgaga
      601 cgatcaggcg cctgaaggcg cagctcgcca gcctgggagc cggccagccc gtggccatcg
      661 tcggcgtggg catgcggttc ccgggggcga tcgatagcct cgagtcgtac tggcaggcgc
      721 tgtccgaggg ccgcgatctg gtgcgcgcga tgccggcggc gcgcaaggcg ccgttcgccg
      781 cggagtggga gaccttgccc cacaagggcg ggttcctcga cgaggtcacc cgcttcgacg
      841 ccgcgttctt cgggatcagc ccgcgcgagg cgcgcgcgct cgatccgcag caccgcctgc
      901 tgctcgaggt cgcctgggag gcgctcgaga acgcggcgct accgcccgat cgcctgaccg
      961 gcgtccggac cggtctctac gtcgggatca ccggccagga ttaccgtgac tggcaggtcg
     1021 gcgcgcccga tgcctactgg gcaaccggca acggtcactg cttcgcggcc ggacggatcg
     1081 cgtacgcgat ggggttcacc gggccggcga tcgcggtcga taccgcgtgc tcgtcggggc
     1141 tggtcgccgt tcacctcgcg agccaggcga tccggcgcgg cgagtgcgac gtggcggtgg
     1201 ccggcggcgt caacctcgtg ctgtcgccgc gctcgacccg gctgctggtg gagactcgtg
     1261 cgctcgcccc cgacgggctg tgcaagacgt tcgacgcgcg cgcgaacggc ttcgcccgcg
     1321 gcgagggctg cggcatcgtc gtcctgaagc gcctggacca cgcgctgcgc gaccgcgatc
     1381 acgtgcacgc ggtgatccac ggctcggcgg tcaaccagga cggccgatcc tcgggcttca
     1441 ccgcgccgaa cgtgcgcgca cagatcgcgc tgatcgaggc ggcgctcgtg gatgcggggc
     1501 tgaccccggc cgacatcggg ctcatcgagg cacacggcac cgggacggcg ctgggcgatc
     1561 cgatcgagat ggaggcgctc gtcgcggcgc tcggccggaa caacgccggt gcgacgctgt
     1621 atgtcggatc ggtcaagccc aacatcgggc acctcgaagc cgcggccggc atcgccggcc
     1681 tgctcaaggc cgtcgcgtgc tgcgcgcacc gcgcgatccc ggcgctcgtg caccaccgga
     1741 cgctgagccc gcggatcgat ctcgcgggca ccgggatccg cttgccgacg acgctggtgc
     1801 cgtggacgac ggacgtcggg cggttcgccg gggtcagctc gtttggcctc agcggaacca
     1861 acgcgcacat gatcctcggg gccggcgatc cgggcggcgc tcgcccggcg cagggggcaa
     1921 ggggcttcga gctcacggcg cggacaccgg aggcgctgcg tgcgctggcc gcgcggttcc
     1981 gccatcgcct cgccgcgctt cccgacgacc agtatggcgc gttcgcgtac accgcgacct
     2041 cgggccggac gcggcacccc gtgcgggcgc ggatcgcagc gtccgacaag accgcggcgc
     2101 tcgcagcgct cgacgcgctc gccggcaaca tgccttgccc tgccgtgcgg ctcggcgcgg
     2161 gcgaggaacc gttcgaggat ctcccgcggt gcgtcgtggt gctgccgcac tatccgtggc
     2221 agcgcgagca ccacgctcca cagccggcgg tggagtcggc gtcggtcggt acggcacgcg
     2281 caacctcggc gccgcccgcg tccgaccggc tcgacgccac gcgcacccag gtgcgccgcc
     2341 acgtggcgac cgtcctgggc caccacgacg cgagcgcggt gcccgacgac gcgagcctgt
     2401 tcgacctcgg gctcgactcg atgatggcgg tcgacctggc gtgcgcgctc ggctcggcgc
     2461 tcggggtcga gctgtcgctc acccacgtgc tgtcacaccc gacggtgaat ggcctcgcag
     2521 ccacgatcgt cgcgggcctg ccggtcccgg cgccgagccc ggcgcgtgca ccggtcgccg
     2581 ctgcgagcga gcccgcgcat cccgccgcgc gaccgaccgc cggccccatg cgtgctccgc
     2641 gcgtggcgtt cctgttctcg tgccagggca gtcagtactt cggcatgggt cgagagctct
     2701 acaacaccga gccggtgttc cgggcgcgga tcgacgcgtg cgatcgcatc cttgccccga
     2761 tgctcggggc ttcgctcgag gcgctgatga tgcacggcga cgaccgcgac gcgatccacc
     2821 acacccgcgt ggcccagccg gcgctcgtcg cgctccagct cgcgctcgcc gatctatgga
     2881 agtcgtgggg ggtgaccccg gcggtcgtca tgggccacag cctcggcgag gtggccgccg
     2941 cgatccacgc cggggtcatg gacctcgtgt ccgggctcac cctggtcgcg catcgcgcgc
     3001 gtctcatggc gtcgacgccg cgcggggcga tgctcgccgt gactgcgccg ctggcgcagg
     3061 tccgtgcgtg gctcgcggga accagcatcg acgtcgccgc gatcaacggc ccggagtcca
     3121 tcgtggtgtc gggcgcgcac gacgcgatcg aggcactcac cgcccggctg cacgccgagg
     3181 gggttgtcgt gcgcccgctc gccgtcccac tggcatcgca ctcgcgggtg atggagccga
     3241 tcgtgcgccc gctgcacgac gcgatcgcgc acctcgcgtt ccatccgccg accctgccca
     3301 tcctctcgaa cctgaccggc cagctcgccg ctgccgatca gtacggtgcg gagtactgga
     3361 gccggcacct gtgcgagccg gtgcggttcc acgacggggt ccaggcgctt cgcgccctcg
     3421 acatcgacgt ctgcctggag atcggccccg atggcacgct cgcaaacctc gtcatggcca
     3481 ccgggctcct gaccggcggg gccagcgtcg cctccctccg tcgcggcgcc agcgaccgcg
     3541 ccagcatgct cggcgccgtc gatgcgctcc atgcccgagg ccagcagttc gcatggagcg
     3601 aggtcctggc ggtctcgaga gcgaagccga gtgcgatcgc cccgtggccg ctggtcgcaa
     3661 gcatctccgg ggcgatggcc gacatccgcc gtggcgacaa gcggggggca gcatgagctt
     3721 cttcgtgagg ccgcatccgc ttgcgaaccc atcgctccgc gtgatcgcgt tccatcacgc
     3781 cggtgggtcc gccgcgatgt accacccgat gagcgccggg atgccggccg gctgggagct
     3841 gctcctcctc gacctgccgg ggcgcggcaa gcggtacgcc gaggagccac tccgcaccac
     3901 cccggcgctc gtcgcgcgcg tcgtggagga cgtgcgtccg tggctcgaca tcccggtcgc
     3961 gctgttcggt cacagcctcg gcgccatcct cgccgcggag gtggggcgcg cgtgcgagca
     4021 gctgggcgca ccgccggtct gggtaggcgt ctcggggcgc gtcgctccga ccctccaggc
     4081 gcatgctctc cgcctgaacg acctcgatga cgcgtcgctg ctcgccgagg tgctcgatct
     4141 cggcggaacg ccggatcggc tgatcgagat tcccgagctg cgcacgcact tcctgcgcgt
     4201 cgtccgcgcc gacatcgcgg tgctcgactc gtaccagcct gctccggacc gcgcggcgct
     4261 cggttgcccg atgaccgcgt tcgccggcac gggcgacgtg tgggctcccc cggccacgat
     4321 gcgcccgtgg gcgcgcgaga cgtgcagcga cttctgtctg cgccagttcg aggggggcca
     4381 cttctacttc ctcgggccgg cgttcgcccg gttcacgcgc gagctcgttc acgagatcga
     4441 gccgtatgcc gatgcgctcg ccggtccaag ctaagacgtg gacgcgtagg ctccgtcgcc
     4501 ccgacaggat gcgcggcggg gtgttcgtga ccctggagga cacacacgtt gcgccacctg
     4561 ccgaggccgc gccgaggtgg ccacctggcc gacgcgtgtc gtaggcgctc accgagggtg
     4621 actggccgtg cagcctcacg ctcgtaccga ccgcgctgca tggcgcggtc tctcacgctg
     4681 gcggtgctgc caagttcacg gatcggacag ggatacctta tgatgactag ctggccagcc
     4741 atggaacgac aacggcaggg agaccgagat gagtgatagt acgtcacgcc agccagagcg
     4801 gtcctccggg accgctgctg ccagtgtgcc ggatcgcctc tgggctgttc cgatctacct
     4861 cccgtatctg caacccgcgc tcaccgatcg cggtgt
//
LOCUS       DASVOR010001719         1727 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_193271,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001719 DASVOR010000000
VERSION     DASVOR010001719.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193271"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..>1727)
                     /locus_tag="VF516_32085"
     CDS             complement(93..>1727)
                     /locus_tag="VF516_32085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112429.1"
                     /translation="DALEAVLRRGPAAPYWGPAHRRMVDVALETRDHAGVLARLEAIK
                     TADPIPPSAAGERSYLRGRAAYDAGKLADAEGELVLISKQSRLYSSAVYLRAVIRARQ
                     GKYRDSAEAMCEVAATEDNDKVTFVVDDRYFTIKDLARLGLGRVAHEQGEYDDAYYHY
                     FQIPEDSAYLPDALFEASWSMYQKRELATARDLVHEFLATFPSSPLWPEASLLAGYVE
                     LADCKFDASQKWYDELTAKLQPIVDEIDRVRKDPELRKQLFARAVTRFHEIKDTGELA
                     GARAAKAAPATAIDQVVALLRLDPRFLRLSDAITGMHEQATTSAAVVRQWQQLARQVG
                     ETKVAAISTTKTLEQEQLADANAVVEDLRRLIAQIRDEKSELARARREGVIAAGDAAE
                     QDARLDKLNDRVRTLEKRALAAADHAAEAATTQAAPGIKPLIQADITDARRLDKAAHA
                     LLDKLGQAGDKLAQQAVDRLYADTRRVLDKAKLGKIDAIIGQKRKLDIEVQDLAAGRF
                     PEELIGRMWNASMIGDDEEYWPWQGEYWADEYEGWR"
BASE COUNT          200 a          608 c          642 g          277 t
ORIGIN      
        1 cggccagccg gcggcggcca cggccagcgc cagcgccgcg cggcggagcg gccgccgggg
       61 cgtggaacgc ggggtggacc gatcggatcc cgtcatcgcc agccctcgta ttcgtcggcc
      121 cagtattcgc cttgccacgg ccagtactcc tcgtcgtcgc cgatcatgct cgcgttccac
      181 atccggccga tcagctcctc ggggaaccgc ccggccgcga ggtcctggac ctcgatgtcg
      241 agcttgcgct tctggccgat gatcgcgtcg atcttgccca gcttggcctt gtcgagcacg
      301 cggcgggtgt cggcgtacag ccggtcgacc gcctgctggg cgagcttgtc gccggcctgg
      361 ccgagcttgt cgagcagcgc gtgcgcggcc ttgtcgagcc ggcgcgcgtc ggtgatgtcg
      421 gcctggatca gcggcttgat gcccggcgcc gcctgggtgg tcgcggcctc ggcggcgtga
      481 tcggcggcgg cgagcgcgcg cttctccagc gtgcgcaccc gatcgttcag cttgtcgagc
      541 cgggcgtcct gctcggcggc gtcgccggcg gcgatcacgc cctcgcgccg ggcgcgcgcg
      601 agctcggact tctcgtcccg gatctgcgcg atcagccggc gcaggtcctc gaccaccgcg
      661 ttggcgtcgg cgagctgctc ctgctcgagg gtcttggtgg tcgagatcgc ggcgaccttc
      721 gtctcgccga cctggcgcgc gagctgctgc cactggcgga ccaccgcggc cgacgtcgtc
      781 gcctgctcgt gcatgccggt gatcgcgtcg ctgagccgga ggaaccgggg atcgaggcgg
      841 agcagggcga ccacctgatc gatcgccgtg gcgggcgcgg ccttggcggc cctggcgccg
      901 gcgagctcgc cggtgtcctt gatctcgtgg aaccgggtga ccgcgcgggc gaacagctgc
      961 ttgcgcagct cggggtcctt gcgcacccgg tcgatctcgt cgacgatcgg ctggagcttg
     1021 gcggtcagct cgtcgtacca cttctgcgat gcgtcgaact tgcagtcggc gagctcgacg
     1081 tagccggcga gcaggctggc ctcgggccac agcggcgacg acgggaacgt cgcgaggaac
     1141 tcgtggacca ggtcgcgcgc ggtcgccagc tcgcgcttct ggtacatcga ccacgacgcc
     1201 tcgaacagcg cgtcgggcag gtacgccgag tcctcgggga tctggaagta gtggtagtag
     1261 gcgtcgtcgt actcgccctg ctcgtgggcg acccggccca ggcccagccg cgcgaggtcc
     1321 ttgatcgtga agtagcggtc gtcgaccacg aacgtcacct tgtcgttgtc ctcggtcgcc
     1381 gcgacctcgc acatcgcctc ggccgagtcc cggtacttgc cctggcgggc gcggatcacg
     1441 gcgcgcaggt agaccgccga cgagtacagc cggctctgct tggagatcag cacgagctcg
     1501 ccctcggcgt cggccagctt gccggcgtcg taggcggcgc ggccgcgcag gtagctgcgc
     1561 tcgccggccg ccgacggcgg gatcggatca gcggtcttga tcgcctcgag gcgcgccagc
     1621 acgccggcgt ggtcgcgggt ctcgagcgcg acgtcgacca tgcggcggtg cgccgggccc
     1681 cagtacggcg cggcgggacc gcggcggagc acggcctcga gcgcgtc
//
LOCUS       DASVOR010001720         1552 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_455841,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001720 DASVOR010000000
VERSION     DASVOR010001720.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1552
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_455841"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..660
                     /locus_tag="VF516_32090"
     CDS             <1..660
                     /locus_tag="VF516_32090"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8112430.1"
                     /translation="SEALAIAGRAVRARDAHDARQRVLFTRREWAVTCLVVGCATAFL
                     AGCKEQQRPPARRAPDTQPPPDAEVALRINGKSHRLTLDPRTSLLDALRERIGLTGTK
                     KGCDMGQCGACTVLVGGKRVASCLTLAVMAQGKDITTIEGLADGGALHPMQRAFVDHD
                     GLQCGYCTPGQIMSAVGLLGEGRAHSDDEVREQMSGNLCRCGAYPNIIAAIQAARRTL
                     G"
     gene            681..>1552
                     /locus_tag="VF516_32095"
     CDS             681..>1552
                     /locus_tag="VF516_32095"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX8112431.1"
                     /translation="MQPFAYVHADSIDHALASARRGDAHYIAGGTTLVDLMRLEVMRP
                     TAVVDITGLTDPGLTAIEGTGHGVRIGALATNTDVAYHPLVTTRFPALSAALLSGASP
                     QLRNMATVGGNLLQRTRCPYFRDGTSPCNKRAPGSGCAALDGYTRSHAVLGTSPRCIA
                     THPSDMCVALVALDAVVHTRGPSGNRAIPIAELHTLPGAHPEVESVLVPGELVTHVEL
                     PATPLAARSRYTKVRDRASFSFALASAAIALELQGGAIRDARIALGGLATKPWRAVEA
                     ERWLIGKRAALDSFR"
BASE COUNT          204 a          603 c          557 g          188 t
ORIGIN      
        1 agcgaggcgc tcgcgatcgc cggccgcgcc gtgcgcgcgc gcgacgccca cgacgcccgc
       61 cagcgcgtgc tgttcacccg gcgcgagtgg gcggtgacct gcctggtggt cggctgcgcc
      121 accgcgttcc tcgccggctg caaggaacag cagcggcctc ccgcgcgccg cgcccccgac
      181 acccagccgc cgcccgacgc cgaggtcgcg ctgcggatca acggcaagtc gcaccggctg
      241 acgctcgatc cccggacgtc gctgctcgac gcgctgcgcg agcggatcgg gctgaccggc
      301 accaagaagg gctgcgacat gggccagtgc ggcgcgtgca ccgtgctggt cggcggcaag
      361 cgggttgcgt cgtgcctgac gctcgcggtg atggcgcagg gcaaggacat cacgacgatc
      421 gagggcctcg ccgacggcgg cgcgctgcac ccgatgcagc gcgcgttcgt cgaccacgac
      481 gggctgcagt gcggctactg cacgccgggc cagatcatgt cggcggtcgg cctgctcggc
      541 gagggccgcg cgcacagcga cgacgaggtc cgcgagcaga tgagcggcaa cctgtgccgg
      601 tgcggcgcgt accccaacat catcgccgcg atccaggccg cgcgccggac cctcggctga
      661 cggcggccga aggagaatcg atgcagccgt tcgcctatgt ccatgcagac tcgatcgacc
      721 acgcgctggc cagcgcccgg cgcggcgacg cccactacat cgccggcggc accacgctgg
      781 tcgacctgat gcggctcgag gtgatgcggc cgaccgcggt ggtcgacatc accggcctga
      841 ccgaccccgg gctgaccgcg atcgagggca ccggccacgg cgtgcggatc ggcgcgctcg
      901 cgaccaacac cgacgtcgcg taccacccgc tggtcaccac gcggttcccc gcgctgtcgg
      961 cggccctgct gtccggcgcg tcgccccagc tgcgcaacat ggcgacggtc ggcggcaacc
     1021 tcttgcagcg cacgcgctgc ccgtacttcc gcgacgggac ctcgccgtgc aacaagcgcg
     1081 cgcccggctc gggctgcgcg gcgctcgacg gctacacccg gtcgcacgcg gtgctcggca
     1141 ccagcccgcg ctgcatcgcg acccacccgt cggacatgtg cgtcgcgctg gtcgcgctcg
     1201 acgcggtcgt ccacacccgg ggcccgtcgg gcaaccgcgc gatcccgatc gccgagctcc
     1261 acaccctgcc cggcgcccac cccgaggtcg agagcgtgct ggtgcccggc gagctggtga
     1321 cccacgtcga gctgccggcg acgccgctgg cggcgcgctc gcgctacacc aaggtccgcg
     1381 atcgcgcgtc gttctcgttc gcgctggcgt ccgcggcgat cgcgctcgag ctccagggcg
     1441 gggcgatccg cgacgcccgg atcgcgctcg gcgggctcgc caccaagccg tggcgggcgg
     1501 tcgaggccga gcgctggctg atcggcaagc gggccgcgct cgacagcttc cg
//
LOCUS       DASVOR010001721         1956 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_47264,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001721 DASVOR010000000
VERSION     DASVOR010001721.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1956
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47264"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VF516_32100"
     CDS             <1..187
                     /locus_tag="VF516_32100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112432.1"
                     /translation="CAIRLAQRIGDHPDPGDGAARMRSDFGAVLSAATGATPTRISPT
                     LQARVFIRVICALVLDL"
     gene            756..1520
                     /locus_tag="VF516_32105"
     CDS             756..1520
                     /locus_tag="VF516_32105"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX8112433.1"
                     /translation="MKVGLFIPCYVDQCYPNVGLATVDLLERHGVEVDYPEAQTCCGQ
                     PMANSGLGDAARPLAERFVRIFGDYDHVVCPSGSCAAMVRVHYRGLLAEAASQELTER
                     LARLARSTHELCEFLVDVLKVERASGRFAHRVGLHHSCHGLRELRLGSGSERRIPRFD
                     KIGRLLGGLAGIELVEPARPDECCGFGGTFAVTEDAVSCAMGRDRLAAHERAGAEIVT
                     AADMSCLMHLDGLIRRDRRPLRVMHVAEILAEAAAG"
     gene            complement(1525..>1956)
                     /locus_tag="VF516_32110"
     CDS             complement(1525..>1956)
                     /locus_tag="VF516_32110"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LUD domain-containing protein"
                     /protein_id="HEX8112434.1"
                     /translation="ADAALAALPQAASAVRVVSLVPGVGRPTVELTAVADPHTLADLD
                     LAILPGELGVAENAAVWVEGRALHHRAVFVIANHLALVIHARDIVHHMHEAYDRLAGR
                     PRGYGLFISGPSKTADIEQVLVVGAQGARTCTVLVIDDPAG"
BASE COUNT          253 a          672 c          721 g          310 t
ORIGIN      
        1 ctgcgcgatc cgcttggccc agcgcatcgg cgaccacccc gatccgggcg acggcgccgc
       61 gcgcatgcgc agcgatttcg gcgcggtgtt gtcggcggcg accggcgcga caccgacgag
      121 gatcagtcca acactgcagg cgagagtctt cattcgtgtc atttgtgccc tcgttctcga
      181 tctctagttg ccatatcggg tgatgttgtc tggatgaccc gcgctaactt gcgatgacgg
      241 tgttcgggtg gctgatgcac gagtcgcctg ctgcttgtct cattgtctgg agtccgatcc
      301 aagactcgcg tcattcaccg gcagagtccg gcaagcctgt gcgtgcgggc tcacgctcga
      361 cggtgagcgc cgctgtccat cgcgggcggg agcgcggccc gcgcaccaca ccctgcgtcg
      421 catcccgatc ggtagatcgg agtcagcgcg tctctgagcg agtgtcacga gggcgcgaga
      481 cccgggcgcc gcgatcgcac ctcagcggtg tcgcattggc cgagtgctct gtgtccattt
      541 gctccggatc gcagatcggc attggggcac acgcggtgag ccgccatcgg cggtcgcggc
      601 gcgcgcaccg cagctcgtgt cgcagcggcg agggctgcgt ggcacgcagc cccgcggtgt
      661 cctcagctga ggcgtgaacc ccatgagcgg cgtcgcacgc cgtgtctcac ccggcttgtc
      721 acggcccggc gccggggcaa tgatgctcgg cgcacatgaa ggtaggcctg ttcatcccgt
      781 gctacgtcga ccagtgctac ccgaacgtcg ggctggcgac ggtcgatctg ctcgagcggc
      841 acggtgtgga ggtcgattat ccggaggccc agacctgctg cggccagccg atggccaact
      901 cgggcctggg cgacgcggcc cggccgctgg ccgagcggtt cgtgcggatc ttcggcgact
      961 acgaccacgt ggtgtgcccg tcgggcagct gcgcggcgat ggtccgcgtt cattatcgcg
     1021 gcttgctcgc cgaagcggcg tcgcaggagc tgaccgagcg cctcgctcgg ctcgcgcgct
     1081 ccacccacga gctgtgcgag ttcctggtcg atgtcctcaa ggtcgagcgg gcgtccggcc
     1141 ggtttgccca ccgggtgggc ctgcatcaca gctgccatgg cctgcgcgag ctgcggctgg
     1201 gcagcgggtc ggagcgccgg atcccccggt tcgacaagat cggccggctg ctcgggggcc
     1261 tggcggggat cgagctggtg gagccggcgc gccccgacga gtgctgcggg ttcggcggca
     1321 cgttcgccgt caccgaggac gcggtgtcgt gcgcgatggg ccgcgatcgg ctcgccgcgc
     1381 acgagcgcgc cggggccgag atcgtgaccg ccgcggacat gtcgtgcctg atgcacctcg
     1441 acggcctgat ccggcgcgac cggcgcccgc tgcgcgtgat gcacgtcgcc gagatcctcg
     1501 ccgaggcggc ggcgggctga tcggctatcc cgccgggtcg tcgatcacca gcaccgtgca
     1561 ggtccgcgcg ccctgcgcgc cgaccacgag cacctgctcg atgtcggcgg tcttcgacgg
     1621 gccggagatg aacagcccgt agccgcgcgg ccggccggcg agccggtcgt aggcctcgtg
     1681 catgtggtgc acgatgtcgc gcgcgtggat cacgagcgcg aggtggttgg cgatcacgaa
     1741 caccgcgcgg tggtgcagcg cgcggccctc gacccacacc gcggcgttct ccgcgacgcc
     1801 gagctcgccg ggcaggatcg cgaggtcgag gtcggccagc gtgtgcggat ctgcgacggc
     1861 ggtgagctcg acggtgggcc ggcccacccc cggcacgagc gacaccacgc gcaccgccga
     1921 ggcggcctgc ggcagcgccg cgagcgcggc gtcggc
//
LOCUS       DASVOR010001722         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_222319,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001722 DASVOR010000000
VERSION     DASVOR010001722.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222319"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VF516_32115"
     CDS             complement(<1..193)
                     /locus_tag="VF516_32115"
                     /inference="COORDINATES: protein motif:HMM:NF025802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RING finger protein"
                     /protein_id="HEX8112435.1"
                     /translation="MRTLKANLKLEGKPCGWCQAPLALGDDAAVCTACEAAHHQGCWD
                     TKAGCATHGCTSAPLRRLDA"
     gene            complement(224..1582)
                     /locus_tag="VF516_32120"
     CDS             complement(224..1582)
                     /locus_tag="VF516_32120"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8112436.1"
                     /translation="MTEPPIRIERGELFDPAVDKALARERAGRERVVADAPPVSPLRR
                     LLLNSMFYLPLAAVLGAFITWRLLEPHISDQRIIGGEIVLVNTEPFDLPRGFIALTVG
                     SHEVLIDPGRVKFEAGVHGESPLASADDLKVGARIEAVGLPEGHRLIAAAIRPTDNPA
                     SRGEVERPEWPLFLLFPLTAALIAFGLLLSEGITTRNWIRMIERTLLGSFLATLFTVL
                     AFIPAGLILQLSHLVMSSEVHKHPDLLVVTIKDVGGVSFLLFTACRSAAWACIGAAAG
                     FGMNLVRSTRTQLRNSVIGGALGGALGGMFFDPIDRWFSSSLFVGSSTSRGVGTLAVG
                     LSIGIFVALVERLGRDAWLRVRTGPLAGKSFILYKTPSILGSAPQSDVYLYKDADIDA
                     SHVAIHRVGTIYEIEDLGTRMGTSVGGAQIRRRRLVSGDQIIIGSTILDFEERQKRTP
                     AA"
     gene            complement(1579..>1943)
                     /locus_tag="VF516_32125"
     CDS             complement(1579..>1943)
                     /locus_tag="VF516_32125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112437.1"
                     /translation="ISFAAQLRTARTRLIQLEQARGTAQAVLTQLELAARRGNEPSKE
                     VTRDLAEQRAGLGAIYAELAADCAKADPGRARRLLAAAAHLDPGNRVRYEKQLQMFDP
                     TGHLPANPGEAAPQGEPR"
BASE COUNT          289 a          667 c          702 g          285 t
ORIGIN      
        1 cggcatcgag ccggcgcagc ggcgcgctgg tgcagccgtg cgtcgcgcag ccggccttgg
       61 tgtcccagca accctggtgg tgcgccgcct cgcaggcggt gcacaccgcg gcatcgtcgc
      121 cgagcgcgag cggtgcctga caccacccgc agggcttgcc ttcgagcttc aagttcgctt
      181 tgagggtccg cacaggttcc atgacagtcc tctcttctcg tgctcacgcc gccggcgtgc
      241 gcttctgccg ctcctcgaaa tcgaggatcg tgcttccgat gatgatctga tcgcccgaga
      301 ccagccggcg gcggcggatc tgggccccac cgaccgacgt gcccatgcgc gttccgagat
      361 cctcgatctc gtagatcgtg ccgacccggt ggatcgcgac gtgcgatgca tcgatgtcgg
      421 cgtccttgta caggtagacg tcggactgcg gcgcgctgcc caggatgctc ggggtcttgt
      481 agaggatgaa cgacttgccg gccagcgggc cggtccgcac gcgcagccag gcgtcgcggc
      541 ccagccgctc gaccagcgcc acgaagatgc cgatcgacag cccgaccgcg agcgtgccga
      601 cgccgcgcga cgtcgagctc ccgacgaaca gcgacgagct gaaccagcgg tcgatcggat
      661 cgaagaacat cccgccgagc gcaccgccga gcgcgccgcc gatcaccgag ttgcggagct
      721 gcgtgcgggt cgagcgcacc aggttcatgc cgaagccggc cgccgcgccg atgcacgccc
      781 acgcggcgct gcggcacgcc gtgaacagca ggaagctgac cccgccgacg tccttgatcg
      841 tgaccacgag caggtccgga tgcttgtgga cctccgacga catcaccagg tgcgagagct
      901 ggaggatcag ccccgcgggg atgaacgcga gcacggtgaa cagcgtggcg aggaagctgc
      961 cgagcagcgt gcgctcgatc atccggatcc agttccgggt cgtgatgccc tcggacagca
     1021 gcaggccgaa cgcgatcagg gctgccgtca gcgggaacag caagaacagc ggccattcgg
     1081 gacgctcgac ctcgccgcgc gacgccgggt tgtcggtcgg acggatcgcg gcggcgatca
     1141 ggcggtgccc ctccggcagc ccgaccgctt cgatccgcgc gccgaccttg aggtcatcgg
     1201 cgctggcgag cggcgactcg ccgtggacgc cggcctcgaa cttgacgcgc cccggatcga
     1261 tcagcacctc gtgggatccc acggtcagcg cgatgaagcc gcgcggcagg tcgaacggct
     1321 cggtgttgac caggacgatc tccccaccga tgatccgctg atcgctgatg tggggctcga
     1381 gcagccgcca ggtgatgaac gcgcccagca ccgcggcgag cggcaggtag aacatgctgt
     1441 tgagcagcag ccggcgcagc ggtgacacgg gcggtgcgtc ggcgaccacg cgctcgcgtc
     1501 ccgcgcgctc gcgggcgagc gccttgtcga ccgcgggatc gaacagctcg ccgcgctcga
     1561 tccggatcgg tggttcggtc atcgtggctc tccttgtggc gctgcttcgc cgggattggc
     1621 gggcaagtgc cccgtcgggt cgaacatctg cagctgcttc tcgtagcgga cgcggttgcc
     1681 gggatcgaga tgggcggccg cggcgagcag ccggcgcgcg cggcccggat cggccttggc
     1741 gcagtcggcg gcgagctcgg cgtagatcgc gcccaggccg gcgcgctgct ccgcgaggtc
     1801 gcgggtgacc tccttcgatg gttcgttgcc gcggcgggcc gcgagctcga gctgggtgag
     1861 cacggcctgt gcagtgccgc gggcctgctc gagctggatc aggcgggtgc gcgcggtccg
     1921 cagctgcgcg gcgaagctga tgc
//
LOCUS       DASVOR010001723         2358 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_106279,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001723 DASVOR010000000
VERSION     DASVOR010001723.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2358
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106279"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..985)
                     /locus_tag="VF516_32130"
     CDS             complement(<1..985)
                     /locus_tag="VF516_32130"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112438.1"
                     /translation="MEAASGSRQLSEGTMLDDKYRIERLLATGGMGAVYLGMHVGLRK
                     RVAIKVLNPQLNSAPMLERFHREAITASQIGHEGIAQVTDLGISAEGEPFLVMEYLEG
                     ESLASRLKATGPLAIEDACELGCAILSPLAAAHHAGIIHRDLKPDNVFLVRQSRGEMV
                     KLLDFGISRAKGQDSSFRLTTTGLVLGTPYYMSPEQARGDSEVTIGADLYSFGVMLYE
                     MLIGVVPIRADNYNQLMYRVMTGEYIRPRQLRRDLPEVLEQIIVRAMALEPADRPSSA
                     HELEAALMPFCRQVFRDAATGRLSAPGLPFRSSAPRADALTPATAPGRPGPA"
     gene            1339..1926
                     /locus_tag="VF516_32135"
     CDS             1339..1926
                     /locus_tag="VF516_32135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEX8112439.1"
                     /translation="MLARLDREMAPIHVEVDQEWHWLLRDRSATRDDYRRQLVVTYGF
                     ESPVELACVHTPGLAHAIDLRGRWRAGLIVQDLLALGAAPEDIPSIQCHSLARFHDPA
                     EALAWMYVIERPTLIHGELRDELTSRFVDVARATAYLGAYDGTASKRWAGLGIALDRV
                     CVSDKVCKRVIDAAHAAYCAWSDWRAGSAPLRSVG"
BASE COUNT          363 a          781 c          826 g          388 t
ORIGIN      
        1 gggccgggcc cggccgaccc ggggccgtcg ccggggtcag cgcatcggcg cgcggcgcgg
       61 acgatcggaa cggcagcccc ggcgcggaca gccggccggt ggcggcatcg cggaacacct
      121 ggcggcagaa cggcatcagc gccgcctcga gctcgtgcgc gctcgacggc cgatcggcgg
      181 gctcgagcgc catggcgcgc acgatgatct gctcgagcac ctcgggcagg tcccgccgca
      241 gctggcgcgg ccggatgtac tcgccggtca tcacccggta catcagctgg ttgtagttgt
      301 ccgcccggat cggaacgaca ccgatcagca tctcgtacag catcacgccg aacgagtaga
      361 ggtccgcccc gatggtgacc tcgctgtcgc cgcgcgcctg ctccggcgac atgtagtacg
      421 gcgtgccgag gacgagcccg gtcgtggtga gccggaagct cgagtcctgg cccttcgcgc
      481 gcgagatccc gaagtcgagc agcttgacca tctcgccccg gctctggcgg accagaaaca
      541 cgttgtcggg cttgagatcg cggtggatga tcccggcatg gtgcgccgcc gccagcggcg
      601 acaggatcgc gcagccgagc tcgcaggcat cctcgatcgc gagcgggccg gtcgccttga
      661 gccggctcgc cagcgactcg ccctcgaggt actccatcac caggaacggc tcgccctcgg
      721 cgctgatccc cagatcggtc acctgcgcga tgccctcgtg gccgatctgc gaggcggtga
      781 tggcctcgcg gtggaaccgc tcgagcatcg gggcgctgtt cagctgtggg ttcagcacct
      841 tgatcgccac ccgtttgcgc agcccgacgt gcataccgag gtagaccgcg cccattccac
      901 cggtcgcgag caggcgctcg atgcggtact tgtcatcgag catcgtgcct tcggacagct
      961 ggcgagatcc cgaggcggct tccacgcggg ctgaaggata agctacgaag cgcgtgaatt
     1021 cacgtcgaat agtcggcctt ggatgagcgt agggtaagac caccgccgct tttgacctcg
     1081 atccctgcga gctgactgcc tcacgtcgga cgcacgcgat cgccagcttt gctgcgcctg
     1141 gctcctgcca atgcgagccg cgtcacgccg cgccgcggcg tccgcccggt tggggaggtc
     1201 tggcggtggt tcctccgcac agcgtgcccc gacgacaccc ttccagccgc cttcgcccaa
     1261 gcgttcttgt accgtgagaa accagtcagt cagcacacgg attgcactgt gcggagcgac
     1321 gtgcaggccg gtggaaccat gctggcgcgc ctcgatcgcg agatggcgcc gatccatgtc
     1381 gaggtggacc aggagtggca ctggttgctg cgcgatcgca gcgcgacgcg cgacgactac
     1441 cgccgccagc tggtcgtcac ctatggcttc gagtcgccgg tcgagctcgc atgtgtgcac
     1501 acacccggcc tggcccacgc gatcgacctt cgcggtcgct ggcgtgccgg gctgatcgtg
     1561 caggacctgc tcgcgctcgg cgcagcgccg gaggacatcc cgagcatcca gtgccattcg
     1621 ctggcgcggt ttcacgaccc ggccgaagcg ctggcctgga tgtacgtcat cgagcgcccg
     1681 acgctgatcc acggcgagct acgcgacgag ctgacgagtc ggttcgtcga cgtcgcgcgc
     1741 gcgaccgcgt acctcggcgc gtacgacggc accgcgagca agcgctgggc cgggctcggc
     1801 atcgcgctgg atcgcgtctg tgtgtcggac aaggtctgca agcgcgtgat cgacgcagcg
     1861 cacgccgcgt actgcgcatg gagcgactgg cgtgcgggca gcgcgccgct gcggagcgtc
     1921 ggctgacgcg ccgcggattg gtgaaggccg ttgaggttga ccttgccgtt gtcgcagagc
     1981 gttgaccttg agggcgacgt agtcgttgag cccgtcgtcg accttggacg tgaaccctgg
     2041 gtcattttgc gaggaatcgt cctcgagaat gacggagatg ggcaaggtcg aggtcgacgg
     2101 tggggtcgac gtcctacgtc gccgtcaagg cccacggtgg agtcaacgac gaggatggaa
     2161 tatcgagtgc gatgagacga tattcgttct tcttcgacga agaaattcat tctgtgaaga
     2221 tccggtgacc ggcgggatct gcgatccagc atggattgcc atcgccgatc cggtctgcgc
     2281 tgtgtggccg gatcgcgcgc ccgccgagcc ggccggggat ccgccggggc gtcgtggggc
     2341 gggccggtga cgcccggc
//
LOCUS       DASVOR010001724         4149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_222338,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001724 DASVOR010000000
VERSION     DASVOR010001724.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222338"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..511
                     /locus_tag="VF516_32140"
     CDS             224..511
                     /locus_tag="VF516_32140"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEX8112440.1"
                     /translation="MPMPLEIIGLGKREVRFVWDEGDEDVWGARELRIRCSCAHCQSE
                     ATGERLLDPATVPADLTVKDMHLVGNYGIGIHFSDDHTTGIFRFRELKRAR"
     gene            788..2275
                     /locus_tag="VF516_32145"
     CDS             788..2275
                     /locus_tag="VF516_32145"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8112441.1"
                     /translation="MKSRVVFVVGMVICALAIRPAHGQPADALKKAQAAFDKAQLDYL
                     QGKYDEAAQGFVDAFAARQFPQFLYNAGASYHMKGKKASDPDAYQKAVDAYRQYLDKD
                     PQAADKAKVEKAIGVLEAEIKRLKEQPGAGTGSAAGTPTGSAAPPAAPSQEVEQLGDA
                     KPRGLVVVESDPSNATIYLDDKRKGPFATTPWSGSLEGDHKIIIEKRGYTVVERPVSA
                     DPSKLFVLSAAMSQQSFLGWVEITSNVPGADIFIDDKSVGAVGRTPLSQNIKPGKHTF
                     WISADGYDEYRQEVEVIPNETHAIKAQLKGSPVGKLNVTGLGIEDATIFVDGKVLCER
                     GPCLKSLQQGEHTITVTRPDTKPYTKRVTIQARTETQVRITLAPTPSRSDAIVAYVLA
                     AGFGGGGIVLGMQANKYRDDLRKEIAAGNPPVDSNDPRFTKGKIFAIAADAAYAVAGI
                     TALTAVYYTFRDKGAPSTGLIDVRALALRPEFGPGYAGLGMGVSF"
     gene            2275..3498
                     /locus_tag="VF516_32150"
     CDS             2275..3498
                     /locus_tag="VF516_32150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112442.1"
                     /translation="MRTTMLFLAAGAAALGACKWTEFDDLQNQTWVGSTEKPNVKSSD
                     YGVAIQRGKPLTDGGTLVVIGANQPTYSELAYDANGGSKLTANTLELNSQYGIGTLDT
                     QPILLADPTTDDIALVVNGGGSQILVLTGAGQLNMHNLFVNPSTVDAATYMLPPGSSA
                     DEPLVASGDVVVGTFYGTVPNPQPTCKLTDSGTAIQAHALGVVRTGAAITDDVIAWGS
                     AGAAAGKLYRYPGSVFNGCAPAGVSTTGHDTGFVPGRGSQILTIDPSHVLLQGHHDAD
                     DTSLLQVYDATLSPVGGSVSLPRLRSAAILDTGSQKYVIAGYPGEIVGGTAAGVVRLF
                     KVSAAGIESQPTALLNDAQPENGESFGRAVAAVPFKGKPVVAVAANNEIFMYFRLNTT
                     DGTSTPIYGETRQGR"
     gene            3500..>4149
                     /locus_tag="VF516_32155"
     CDS             3500..>4149
                     /locus_tag="VF516_32155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112443.1"
                     /translation="MQHLCPTCRRQFGEPGFCPFDGAPLVPGSMCEQETLVSAAMPVA
                     IPVPVPGAGPDQETLTDPQPAPAAGAGPAAPDPRGPPTKVEIRKRTFPLTPTPGSASA
                     SASASASASASASGAARPAPAVSTGEITQTRSRSGPEPAVPHGVRAPSDPPRVKAQSA
                     SQDAADALELLGAPRGGEYDKLIGTTLDGRYYVEKKIGEGGMGVVFAARHAVIERPL"
BASE COUNT          671 a         1534 c         1426 g          518 t
ORIGIN      
        1 gtcgcgatcg gcgtggagcg cgcgcagggc ggcgaccagc gccgaggcac tcgccggcgc
       61 accgtcggag atgatccgac ccggcgcgcg gggcatcgac agggctcggc gctgtgcagc
      121 caccgcggga ccctagcgca agtttccgcg cgcgcggagc cgggaggcca gggttgctgc
      181 gcggcgggat ccgcgagcgt ccggcggatg gtagaacccg cgcatgccca tgccgctgga
      241 gatcatcggc ctcggaaagc gcgaggtccg gttcgtgtgg gacgagggcg acgaggacgt
      301 gtggggcgcc cgcgagctgc ggatccggtg cagctgcgcc cactgccagt ccgaggccac
      361 cggcgagcgg ctgctcgacc ccgcgacggt gcccgccgac ctcacggtca aggacatgca
      421 cctggtcggc aactacggga tcgggatcca cttctccgac gaccacacca ccgggatctt
      481 ccggttccgc gagctcaagc gcgcgcggtg acggcgccgg tcggcggcgg cggaggtggc
      541 gatgatgccc tcgccccgag ggcccacgca cccggccgtc gccgcggagc cgcgacgcgg
      601 ctgtcatacc cgcaccgccc gggcgggccg caactccgcg ttcttcggac cgcgtgtgcg
      661 cggcgtcgcc ggggaggatc accgcgctca tcgccgtaca acggcgcggc cctcgagcga
      721 ccgcgtaggg gcgcggcgtc cgcgggcgtg cgctcgttgt gatatcgtcc gatgctcggg
      781 agagactatg aaatcacgcg tcgtgttcgt ggtcgggatg gtgatctgcg cgctggcgat
      841 ccgcccggcg catggccagc ccgccgatgc gctcaagaag gcgcaggcag cattcgacaa
      901 ggcccagctc gattacttgc agggcaagta cgatgaggcc gcccagggct tcgtcgacgc
      961 attcgcggcg cggcagttcc cgcagttcct gtacaacgcg ggcgccagct accacatgaa
     1021 gggcaagaag gcgtcggatc ccgacgccta ccagaaggcg gtcgacgcct accgccagta
     1081 cctcgacaag gatccccagg ccgccgacaa ggccaaggtc gagaaggcga tcggcgtgct
     1141 ggaggccgag atcaagcggc tcaaggagca gcccggcgcg gggaccggca gcgccgccgg
     1201 cacgccgacc ggcagcgccg caccgccggc cgccccgtcg caggaggtcg agcagctcgg
     1261 cgacgccaag ccgcgcggcc tggtcgtggt cgagagcgat ccgtcgaacg cgacgatcta
     1321 cctcgacgac aagcgcaagg ggccgttcgc gaccacgccg tggtccggca gcctggaggg
     1381 cgatcacaag atcatcatcg agaagcgcgg ctacaccgtg gtcgagcgcc cggtgtccgc
     1441 cgatccgagc aagctgttcg tgctgtcggc ggcgatgtcg cagcagagct tcctcggctg
     1501 ggtcgagatc acgtcgaacg tcccgggcgc cgacatcttc atcgacgaca aatcggtcgg
     1561 cgcggtcggc cgcacgccgc tgtcgcagaa catcaagccc ggcaagcaca cgttctggat
     1621 cagcgccgac ggctacgacg agtaccgcca ggaggtcgag gtcatcccca acgagaccca
     1681 cgcgatcaag gcccagctca agggctcgcc ggtcggcaag ctcaacgtca ccggcctggg
     1741 catcgaggac gcgacgatct tcgtcgacgg caaggtgctg tgcgagcgcg ggccatgcct
     1801 caagagcctg cagcagggcg agcacacgat caccgtgacc cggcccgaca ccaagccgta
     1861 caccaagcgc gtcacgatcc aggccaggac cgagacccag gtccggatca cgctggcgcc
     1921 cacgccgagc cgcagcgacg cgatcgtcgc ttacgtgctc gcggccgggt tcggcggcgg
     1981 cggcatcgtg ctcggcatgc aggccaacaa gtaccgcgac gacctgagga aggagatcgc
     2041 cgccggcaac ccgccggtgg actccaacga cccgcggttc accaagggca agatcttcgc
     2101 gatcgccgcc gacgcggcct acgcggtcgc cgggatcacc gcgctgaccg cggtgtacta
     2161 cacgttccgc gacaagggcg cgccgtcgac cggcctgatc gatgtccgcg cgctggcgct
     2221 gcgacctgaa ttcggacccg gctatgccgg tctcggcatg ggagtgagct tctgatgcgc
     2281 accacgatgc tgttcctcgc cgccggcgcc gcggcgctcg gcgcctgcaa gtggaccgag
     2341 ttcgacgacc tgcagaacca gacctgggtc ggctcgaccg agaagcccaa cgtcaagtcg
     2401 tcggactacg gcgtcgcgat ccagcgcggc aagccgctga ccgacggcgg gacgctggtc
     2461 gtgatcggcg ccaaccagcc gacctactcc gagctggcct acgacgcgaa cggcggctcc
     2521 aagctgaccg ccaacacgct ggagctcaac tcgcagtacg ggatcggcac cctcgacacc
     2581 cagccgatcc tgctcgccga cccgaccacc gacgacatcg cgctggtcgt caacggcggc
     2641 ggcagccaga tcctcgtgct gaccggcgcc ggccagctca acatgcacaa cctgttcgtc
     2701 aacccgagca cggtcgacgc ggcgacctac atgctgccgc cgggcagctc ggccgacgaa
     2761 ccgctggtcg catcgggcga cgtcgtggtc ggcacgttct acggcacggt gcccaacccg
     2821 cagccgacct gcaagctgac cgactccggc acggcgatcc aggcccacgc gctcggcgtg
     2881 gtccgcaccg gcgcggccat caccgacgac gtgatcgcgt ggggctcggc gggcgccgcc
     2941 gccggcaagc tgtaccgcta ccccggcagc gtgttcaacg gctgcgcgcc ggcgggggtc
     3001 agcacgaccg gccacgacac cggcttcgtg cccggccgcg gctcgcagat cctgacgatc
     3061 gaccccagcc acgtcctgct ccagggccat cacgacgccg acgacaccag cctgctccag
     3121 gtctacgacg ccaccctgag cccggtcggc ggctcggtgt cgctgcccag gctgcgcagc
     3181 gcggcgatcc tcgacaccgg cagccagaag tacgtgatcg ccggctatcc cggcgagatc
     3241 gtcggcggca cggccgccgg cgtggtccgg ctgttcaagg tctcggcggc cggcatcgag
     3301 tcccagccca ccgccctctt gaacgacgcg cagcccgaga acggcgagtc gttcggccgc
     3361 gccgtcgcgg cggtcccgtt caagggcaag ccggtcgtcg cggtcgccgc caacaacgag
     3421 atcttcatgt acttccggct caacaccacc gacggcacgt ccacgccgat ctacggcgag
     3481 acccggcaag gacggtgagt tgcagcatct ctgcccgacg tgccgccggc agttcggcga
     3541 acccgggttc tgcccgttcg acggcgcgcc gctcgtcccg ggcagcatgt gcgagcagga
     3601 gacgctggtc tcggcggcga tgccggtggc gattcctgtc ccggtccccg gcgccgggcc
     3661 cgatcaggag acgctgaccg acccccagcc ggccccggcc gccggcgcgg gccctgcggc
     3721 cccggacccg cgcggcccgc cgaccaaggt cgagatccgc aagcggacgt tcccgctcac
     3781 cccgaccccc ggctcagcct cagcctcagc ctcagcctcg gcatcggcat cggcctcggc
     3841 ctcgggcgcc gcgcggcccg cccccgcggt cagcaccggc gagatcaccc agacccgatc
     3901 gcgctctggc cccgagccgg cggtaccgca cggcgtacgc gcgccgtccg acccgccgcg
     3961 ggtcaaggcc cagtcggcat cgcaggacgc ggccgacgcg ctcgagctgc tcggcgcgcc
     4021 gcgcggcggc gagtacgaca agctgatcgg cacgaccctc gacggccgct actacgtcga
     4081 gaagaagatc ggcgagggcg gcatgggcgt ggtgttcgcc gcgcgccacg ccgtgatcga
     4141 gcggccgct
//
LOCUS       DASVOR010001725         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_396954,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001725 DASVOR010000000
VERSION     DASVOR010001725.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396954"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..912)
                     /locus_tag="VF516_32160"
     CDS             complement(16..912)
                     /locus_tag="VF516_32160"
                     /inference="COORDINATES: protein motif:HMM:NF016142.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HEX8112444.1"
                     /translation="MLITDEVREAQATTGAIVALESTILAHGLPPGRNRKVATRLEET
                     IRAAGAVPATIAVLDGEARIGLTGDELDRVCSTELVKLSRRDLASAYALRRDGATTVA
                     ATAAIAAASGIHVFATGGLGGVHRGAADTFDVSADLDVLAETPIVVVCSGVKSILDIA
                     ATLEVLETRSIPVLGYRTATFPAFYLRESAYPAPWTVRTPAEAAAVARAHFGSVPGTA
                     GIVLANPVPREAEMPRGLHDELLAGGLALLAERGITGHDVTPALLEHFHAASGGVSID
                     TNEELVVANAALAAEVAVALAA"
     gene            1255..>1548
                     /locus_tag="VF516_32165"
     CDS             1255..>1548
                     /locus_tag="VF516_32165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112445.1"
                     /translation="MPSTRNRAALPLPRLSASELVAGPPDEGAGAWAGAPSAVLAGDT
                     IYLAYRLRRPIGEGRGYRNVIAASTDGVRFEEVARLERDRFAADSLERPALVLT"
BASE COUNT          184 a          587 c          577 g          200 t
ORIGIN      
        1 ccaccgcgat ccggctcacg cggccagcgc cacggcgacc tcggcggcca gggcggcgtt
       61 cgcgacgacc agctcctcgt tggtgtcgat gctcacgccg ccgctcgccg cgtggaagtg
      121 ttcgagcagc gccggggtca cgtcgtgccc ggtgatcccg cgctcggcga gcagggccag
      181 cccgccggcg agcagctcgt cgtgcagccc gcgcggcatc tccgcctccc gcgggaccgg
      241 gttggccagc acgattccgg cggtgcccgg caccgagccg aagtgcgcgc gggcgaccgc
      301 ggccgcctcg gccggggtcc gcaccgtcca cggcgccggg taggccgact cgcgcaggta
      361 gaacgccggg aacgtcgccg tccggtagcc gagcaccggg atcgatcggg tctccagcac
      421 ctccagggtg gcggcgatgt cgaggatcga cttcacgccc gagcagacca cgacgatcgg
      481 ggtctcggcg agcacgtcga ggtccgccga cacgtcgaag gtgtcggcgg cgccgcggtg
      541 gacgccgccg aggccgccgg tggcgaacac gtggatcccg ctggccgcag cgatcgccgc
      601 cgtcgcggcg accgtcgtcg cgccgtcgcg gcgcagcgcg tacgcggagg cgaggtcgcg
      661 gcgggacagc ttgaccagct cggtgctgca gacgcggtcc agctcgtcgc cggtcaggcc
      721 gatcctggcc tcgccgtcga ggacggcgat ggtcgcgggg acggcacccg cggcgcggat
      781 ggtctcctcc aggcgggtcg cgaccttgcg gttgcggccg ggcgggaggc cgtgggcgag
      841 gatcgtgctc tccagcgcga cgatcgcgcc ggtcgttgcc tgcgcctctc gtacttcgtc
      901 ggtgatcagc acggtgcacc ctctctcgtc gggtgccacg ttatgggggg ccgcccctcc
      961 accccggcag gggctctgcc cccacacccc cggcaggggg ctctgccgca cacccccggc
     1021 aggggctccg ccccctccca cccctggaag gccttcgccc ggatcgggct tcccggcccg
     1081 ggcccccagc cgcgggcgat ccgggacttc tcgagctcgc aggcgcgcag gaagtcggcc
     1141 tccggctccg gccttcggcg cgccgacgag caggaggtcg ggcagggcca ccatccacct
     1201 cgcctcgata atttcagacc aagtcacatg gagatgtatg gcagggttcg cgccgtgccg
     1261 tcgacccgga accgcgccgc cctgccgctg ccccgcctgt ccgcatccga gctggtcgcc
     1321 ggcccccccg acgagggcgc cggcgcctgg gcgggagcac cgagcgcggt gctggccggc
     1381 gacacgatct acctggccta ccggctgcgg cggccgatcg gcgagggtcg cggctaccgc
     1441 aacgtgatcg ccgcgtccac cgacggcgtg cggttcgagg aggtcgcgcg gctggagcgg
     1501 gaccggttcg cggccgactc gctggagcgg ccggcgctcg tgctgacc
//
LOCUS       DASVOR010001726         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_310300,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001726 DASVOR010000000
VERSION     DASVOR010001726.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310300"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VF516_32170"
     CDS             complement(<1..979)
                     /locus_tag="VF516_32170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112446.1"
                     /translation="MMRRIRGLALGTYDAKGADAAHATPNASQVRGLVYAVKDNRVPP
                     NPKQPIQHRLHPIGRPVQVDRTNAIEYVTAMLANRIDRLTRFFKAEDPDAITLVPVPS
                     SSVTAATIQTARFRTLRLCNALAGVGFGHVALLAVQRKPVEGKTKGNRRTADQIVAGL
                     ARTSTPIPRGVAVDSVWSQREVVIENAVLRHQITVLRRRSKRPKLHLVDRLKLLIGAR
                     LVPSWRRAIAIVQPETVLRWHRTGFRLFWRRRSRPRKTSPLPPETLDLIRDMGRGDAC
                     GAPSASAASCSSWEQGQQADHPEIHAQRSRQKRRRRPALGDLLEEPRRTH"
     gene            complement(976..1665)
                     /locus_tag="VF516_32175"
     CDS             complement(976..1665)
                     /locus_tag="VF516_32175"
                     /inference="COORDINATES: protein motif:HMM:NF014533.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEX8112447.1"
                     /translation="MTAGYADEYVPPSRADVVAFSSDRIGVTDGLLLKFPQIHAVGDI
                     SLAARPRIAIVGARKATAEGRRRAAQLARDCARAGIVVVSGLAEGIDHAAHTAAIDHG
                     GKTIAVVGTPLDKVYPAKHAALQHAIYRDHLLLSPFTWGDKFVPSNFPERNRIMARLA
                     MATVIIEASDTSGSLHQAAESVMVGHPVFITAGVLDNPKLTWPRRFIGDDKPLSRVLR
                     TSADVIDFARA"
     gene            complement(1875..>2262)
                     /locus_tag="VF516_32180"
     CDS             complement(1875..>2262)
                     /locus_tag="VF516_32180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112448.1"
                     /translation="FGARLGFPFFPALQHGHDELLLVGVLARVLPEQVLQGIGLSRVV
                     DHLCDRGCDRGLLEPSLQLRHVLLGEDPLARAVRDSDATNAENGEQVICSRLGFPFFP
                     ALQHGHDELLLVGVLARVLPEQVLEL"
BASE COUNT          380 a          736 c          784 g          362 t
ORIGIN      
        1 catgcgttcg gcgtggttct tcaagaaggt cgcccaacgc tggccgccgc cgccgtttct
       61 gccgcgaacg ctgcgcatgt atttctggat ggtccgcttg ctgaccttgc tcccagcttg
      121 agcagctcgc cgcggatgcg ctcggcgccc cacaggcgtc cccgcgcccc atgtcgcgga
      181 tgaggtcgag cgtctcgggt ggcagtggcg acgtcttccg aggccgagat cgccgccgcc
      241 agaacaggcg gaagccggta cgatgccagc gcagaacggt ctccggctgg acgatggcga
      301 tcgcgcgccg ccacgacggc accaggcgcg cgccgatgag aagcttcagc cggtccacga
      361 ggtgcagctt cgggcgcttg ctgcggcgtc gaagaacggt gatctgatgg cggaggacag
      421 cgttctcgat gaccacctcc cgctgggacc agaccgaatc cacggcgacg ccgcggggaa
      481 tcggcgtcga cgtacgcgcg aggcccgcaa cgatctggtc cgcggtgcga cgattcccct
      541 tggtcttacc ctcgacgggc ttccgctgca cggccaggag ggcgacgtga ccgaagccga
      601 cacctgcgag cgcattgcag agccgaagcg tacgaaaccg agccgtctgg atcgtcgctg
      661 cggtgaccga cgaggacggg accgggacga gcgtgatcgc gtcgggatcc tcggccttga
      721 agaacctggt gagcctgtcg atccggttcg cgagcatcgc ggtcacgtac tcgatcgcgt
      781 tcgtccgatc gacctggacc ggccggccga tgggatggag tcggtgctgg atcggttgct
      841 tggggttggg tggcacgcgg ttgtccttaa ccgcgtagac caggccccgc acctgcgatg
      901 cgttcggcgt cgcgtgcgcg gcgtcggcac ctttcgcatc gtaggtgccg agcgcgagtc
      961 ctcggatcct cctcatcacg cgcgcgcgaa atcgatcacg tcagcgctcg tgcgaaggac
     1021 gcggctgagc ggcttgtcgt cgccgatgaa ccgcctgggc caggtgagct tgggattgtc
     1081 aagcacaccc gcggtgatga acaccgggtg ccctaccatc acgctctcgg cggcctgatg
     1141 cagcgagcca ctggtgtcgc tcgcctcgat gatcacggtc gccatcgcga gccgcgccat
     1201 gatccggttg cgctcgggga agttggaggg gacgaacttg tcgccccagg tgaacgggga
     1261 gagcaggagg tggtcgcggt agatggcgtg ctggagcgcc gcgtgcttcg ccgggtacac
     1321 cttgtcgagc ggtgtgccga ccaccgcgat ggtcttgccg ccgtggtcga tcgccgcggt
     1381 gtgcgccgcg tggtcaatgc cttcggcgag ccccgacacg accacgatgc cggcgcgcgc
     1441 gcagtcacgc gcgagctgcg cggcacggcg gcgaccctcg gcggtcgcct tgcgcgcgcc
     1501 gacaatcgcg atgcgaggcc gagccgcgag cgagatgtcg ccgaccgcgt ggatctgcgg
     1561 gaacttgagc aggaggccat ccgttacccc gatgcgatcc gagctgaacg cgacgacgtc
     1621 ggcccggctc ggtggtacgt actcgtcggc gtacccagcg gtcatgtcca gcactgatcg
     1681 aaggtagtcg cccggtctga cactgcggcc gggtgctcgt gaagcaggtg caccagccag
     1741 cgcggtcgcg gcccgcgact cggattctcc atcgccatcc cgacgagcgt cgatgacttt
     1801 ccaccgcccc atgctcaaga tcccgcgcga gcctcgcgaa tcttgccgac tgcaacgcat
     1861 cgccgtcgat cctgctacag ttccagcacc tgctcgggca ggactcgagc caggaccccg
     1921 acgagcagga gttcatcatg gccatgctga agagctggga agaagggaaa gccgaggcgc
     1981 gagcagatga cttgctcacc gttctcagcg ttcgtggcat cgctgtcccg gacggcacgc
     2041 gcgagcggat cctcgccgag aaggacatga cgcagctgaa gcgatggctc gagaaggccg
     2101 cggtcgcatc ctcgatcgca gaggtgatcg acgacgcgag atagcccgat gccctgcagc
     2161 acctgctcgg gcaggactcg agccaggacc ccgacgagca ggagttcatc atggccatgc
     2221 tgaagagctg ggaagaaggg aaagccgagg cgcgcaccga aa
//
LOCUS       DASVOR010001727         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_134502,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001727 DASVOR010000000
VERSION     DASVOR010001727.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134502"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF516_32185"
     CDS             complement(<1..239)
                     /locus_tag="VF516_32185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112449.1"
                     /translation="MRWTAAVPSSRPSSDRIAGAIAAAARLLLAFAWHARRVAAPAVD
                     LSLLRQRSFGIATLAGGLSRIGMNGVPYLLPLMLQ"
     gene            complement(600..>1602)
                     /locus_tag="VF516_32190"
     CDS             complement(600..>1602)
                     /locus_tag="VF516_32190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112450.1"
                     /translation="TSRTKSAPSATSTRSTSPSATSPSEPASGRQLGRRSDCGAADCE
                     HHVRAERDLLDAGDRARVTSEMFAGQPPSIPADPTRIEARIGSRDERFERNYVKPSPS
                     SITRRALHVLEPGILWSLLLIESKRSDAEGRGQRQNRATDTDDRMKRAKAEWVAVAHS
                     IEAIIHQIWSASACSVFTMKHGGIFIRSPLESVRSLEDLAAEAMRNSQLADDQLKKNW
                     DRHILATRTDDIRTKLSILVEVLHTADPSWELGDVADLIVASGKDWIYPPTRLAQEYH
                     YDWTSASRRVEPTRIALADWIRDQLRASRKTKKKPPRVRVAGSSHLSSVGKRSLRRR"
BASE COUNT          268 a          506 c          540 g          288 t
ORIGIN      
        1 atttgcagca tcagcggcag cagataggga acgccgttca tgccgatgcg gctgaggccg
       61 ccggccagcg tcgcgatgcc gaaggagcgc tgccggagca gcgagaggtc gaccgccggc
      121 gccgccactc gacgggcgtg ccaggcgaag gccagcagca atcgcgcggc ggcggcgatc
      181 gcgccggcga tcctgtccga gctcggccgc gaggacggca cggccgcggt ccagcgcatg
      241 gtcgacttcg cgcaaaccgc caacacgccg gcgaccgcgc tgtgccccga tgacggcatt
      301 acggtggaga ccctgatcgc gcgtacgcga cgaccatcgc cgcggcggcg aagacgacga
      361 gaagcacaac gaccgcgaag atcgccgtgc gttctcgcgc ctcgacggtg agttcacccc
      421 cgcggtagcg agcgcggagc gctagatgag ccttgttgga gttcgggcgg gccttgacct
      481 tagcggccag cccgtcggga cgacgacgat gacgagtcat gcctctttgt cgcgccaacc
      541 ggggtgactg catcgggaac tctgaagttt tttgtcgcga tggaccacag aatctcccgt
      601 taccgacgac gaagcgacct tttgcccacg ctgctaagat gcgaacttcc agcgacacga
      661 actcgcggag gtttcttctt cgtcttgcgt gatgcgcgca gctgatcgcg aatccagtcc
      721 gcaagcgcga ttcgtgtggg ctcgacgcgc cgggaagcgc tcgtccagtc atagtggtac
      781 tcctgcgcaa gccgcgtcgg cggatagatc cagtctttcc cgctagccac gatcaggtcc
      841 gcgacatcgc cgagttccca tgagggatcg gccgtgtgta gcacttcaac gaggatcgat
      901 aacttcgtcc gaatgtcgtc ggttcgcgtc gcaaggatgt gacggtccca atttttcttc
      961 aactggtcgt ccgcgagttg actgttgcgc atggcctcgg ctgcaagatc ttcgagggag
     1021 cgaactgact cgaggggcga ccggatgaat atgccgccgt gcttcatcgt gaagacgcta
     1081 caggccgatg cgctccaaat ttggtggatg atcgcctcga tggaatgggc gacggcaacc
     1141 cactcagcct tcgcgcgctt cattcggtca tcggtgtccg tcgcgcgatt ctgacgttgg
     1201 ccgcgaccct cggcatcact tcgcttcgat tcgatcagca gcagcgacca cagaatcccg
     1261 ggctccaaga cgtgcagcgc cctccgtgtg atcgaggacg gcgacggctt gacgtagttc
     1321 cgctcgaacc gctcgtcgcg tgatccgatt cgagcctcga tccgagttgg gtccgcgggg
     1381 atcgagggtg gttggcccgc gaacatttcg gaggtcacgc gcgcgcggtc gccggcgtcg
     1441 agcaggtcgc gttcggcgcg gacatggtgc tcgcagtcgg cggcgccgca atcgctgcgg
     1501 cggccgagct gccgtccgct ggccggttcg ctcggtgagg tcgcgctcgg cgacgtcgag
     1561 cgggtcgatg tcgcgctcgg cgcggacttg gtgcgcgagg tc
//
LOCUS       DASVOR010001728         1539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_396969,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001728 DASVOR010000000
VERSION     DASVOR010001728.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396969"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1539)
                     /locus_tag="VF516_32195"
     CDS             complement(<1..>1539)
                     /locus_tag="VF516_32195"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX8112451.1"
                     /translation="VVDVSELRGGPIDQPIDVHATASLDERATTATVGVGSRGASLVE
                     IEGRLPVSLVDVLARRGQAAGRDTPLEATAKLTSTDAARLLAVFGRTEVTSGRIDGSV
                     TLTGTLGAPKATVRVTASQLTIPPGPHNKPVRVVERLAINGSWDGGTARIDVDGTESG
                     GGALRLAAQVRPDRLRDGTATVKATRFDLVPLLVFAPGPAGGMSGVLDADLRLAGLDL
                     RTAQLAGEIHLRQARIPTAPTIGTLRAATIDALVADHEIRIAADGKLGAGSASLTGTI
                     ALDGAAPNGGKARLTLRKVSPIGSIEPQITADVNATLSRDRNQWKAELVVDHGDVVVP
                     SDRGEKLKPAGAPPDMVFADGRRITRRPMTQQEPTNPIFQVKINLRSTRVESKEFRGL
                     IHGQLEMRADGEAIAMYGGIEAERGDLDLFGRRYQVDRAAVNFDGSLDPLLDIRITYD
                     FPEVTTVTEVRGRLSKPDLVMTSDPGTYSQGQLLGFLLGGEPGGDPQSGPLQSQVATA
                     GESLV"
BASE COUNT          183 a          562 c          577 g          217 t
ORIGIN      
        1 accagcgact cgccggcggt ggcgacctgg ctctgcagcg ggccgctctg cggatcgcct
       61 cccggctcgc cgccgagcag gaagccgagc agctggccct gcgagtaggt gcccggatcg
      121 ctcgtcatca cgagatcggg cttgctgagc cggccacgga cctcggtcac cgtggtgacc
      181 tcggggaagt cgtaggtgat ccggatgtcg agcagcggat cgagcgagcc gtcgaagttg
      241 accgcggcgc gatcgacctg gtagcgccgg ccgaacaggt cgaggtcgcc gcgctcggcc
      301 tcgatcccgc cgtacatcgc gatcgcctcg ccgtcggcgc gcatctcgag ctggccgtgg
      361 atcaggccgc ggaactcctt ggactcgacc cgggtcgacc gcaggttgat cttcacctgg
      421 aagatcgggt tggtcggctc ctgctgggtc atcggccgcc gggtgatgcg ccggccgtcg
      481 gcgaacacca tgtcgggcgg cgcgccggcc ggcttgagct tctcgccgcg gtcgctcggc
      541 acgacgacgt cgccatggtc gaccaccagc tcggccttcc actggttgcg atcgcgcgac
      601 agcgtggcgt tgacgtcggc ggtgatctgc ggctcgatgc tgccgatcgg cgacaccttg
      661 cgcagggtga gccgggcctt gccgccgttg ggcgccgcgc cgtcgagcgc gatcgtgccg
      721 gtgagcgacg cgctgccggc gccgagcttg ccgtcggccg cgatccggat ctcgtgatcg
      781 gcgaccagcg cgtcgatcgt ggccgcgcgc agcgtaccga tggtcggcgc ggtcgggatc
      841 cgcgcctggc gcaggtggat ctcgccggcg agctgggcgg tgcgcagatc gagcccggcg
      901 agccggaggt cggcgtcgag cacgccggac atcccgccgg ccgggccggg ggcgaacacg
      961 agcagcggca ccaggtcgaa ccgggtcgcc ttcaccgtgg ccgtcccgtc gcgcagccgg
     1021 tcgggccgga cctgcgcggc cagccgcagc gccccgccgc ccgactcggt gccgtcgacg
     1081 tcgatccggg cggtcccgcc gtcccagctg ccgttgatcg cgagccgctc gacgacgcgc
     1141 accggcttgt tgtgcggccc cggcgggatc gtgagctggc tggcggtgac ccggacggtc
     1201 gccttcggcg cgcccagcgt cccggtcagc gtgaccgagc cgtcgatccg gccggacgtc
     1261 acctcggtgc ggccgaacac cgcgagcagc cgcgcggcgt cggtcgaggt cagcttcgcg
     1321 gtcgcctcga gcggggtatc gcggcccgcc gcctggccgc gccgcgcgag gacatcgacc
     1381 agcgagaccg gcagccggcc ctcgatctcg accagcgacg cgccgcgcga cccgacgccg
     1441 accgtcgcgg tggtcgcccg ctcgtcgagc gacgcggtgg cgtggacgtc gatcggctgg
     1501 tcgatcggcc cgccgcgcag ctcgctgacg tcgaccacc
//
LOCUS       DASVOR010001729         2045 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_134515,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001729 DASVOR010000000
VERSION     DASVOR010001729.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134515"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1422
                     /gene="thrC"
                     /locus_tag="VF516_32200"
     CDS             82..1422
                     /gene="thrC"
                     /locus_tag="VF516_32200"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX8112452.1"
                     /translation="MSFSAEFRCVSGSRGCAGGYPLDQVIYRCPTCGGLLEVVHDLAA
                     LRTRGPAEWKQRFAERWRSTEEPWGSGVWGKHEWVMPGLRVEHIVTMFEGATPMTRAP
                     RLAAALGLGEVRIKHCGTSHTGSFKDLGMTVLVSMVQQMIAAGRPIRAVACASTGDTS
                     AALAAYAAAAGIRAVVILPRGKISTAQLVQPLAAGALVLAIDTDFDGCMRLVQELAER
                     EGVYLANSMNSLRVEGQKTVAIELCQQLGWQVPDWVVIPGGNLGNVSALGAGFEMLLA
                     LGLVDRRPRICVAQAAHAGPLYASYRAGWADLVPVKAQTTLASAIQIGNPVSFGKAVR
                     ALRAFDGVVEIASEAELADASAQADLDGLFTCPHTGVALAALAKLAARGEIRRDQRAV
                     VISTASGLKFADFKVGYHEAKLAGVASPRHRNVPVELPEQYAAVREALVRGIAG"
     gene            1659..>2045
                     /locus_tag="VF516_32205"
     CDS             1659..>2045
                     /locus_tag="VF516_32205"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX8112453.1"
                     /translation="MRVAGLIIAAVIVAVWAMFRHGPVTAANAEVAVDQPVARLQEVR
                     SVGLDGRRVLSARLREVIATRPGAQLDADRLRSDRDVMERALADLGYLAARVEPAKVT
                     FDAAGAAYVTFAVDQGPMFHLRNVEVT"
BASE COUNT          252 a          721 c          787 g          285 t
ORIGIN      
        1 gggccgccct tgctcggcga cgcggcggcc cgcacctgac gccgggcccg ccgggggccg
       61 cgccagctgc taggatggcg catgagcttc tcggccgagt tccgctgcgt ctcaggttct
      121 cggggctgcg cgggagggta ccccctcgac caggtgatct atcgctgccc gacgtgcggc
      181 ggcctgctcg aggtggtgca cgatctcgcg gcgctccgga cgcgcggccc ggccgagtgg
      241 aagcagcggt tcgcggagcg ctggcggtcg accgaagaac cgtggggctc cggggtgtgg
      301 ggcaagcacg agtgggtcat gccggggctc cgggtcgagc acatcgtgac gatgttcgag
      361 ggcgccacgc cgatgacccg ggcgccgcgc ctggcggccg cgctcgggct cggcgaggtc
      421 cggatcaagc actgcggcac gtcgcacacc ggctcgttca aggacctcgg gatgaccgtg
      481 ctggtgtcga tggtccagca gatgatcgcc gcgggccggc cgatccgcgc ggtggcgtgc
      541 gcgtcgaccg gcgacacgtc ggcggcgctc gccgcctacg ccgccgcggc cgggatccgc
      601 gcggtggtga tcctgccgcg cggcaagatc tcgaccgcgc agctggtgca gccgctcgcc
      661 gccggggcgc tggtgctcgc gatcgacacc gacttcgacg gctgcatgcg gctggtccag
      721 gagctggccg agcgcgaggg cgtgtacctg gcgaactcga tgaactcgct gcgcgtcgag
      781 ggccagaaga cggtggcgat cgagctgtgc cagcagctcg gctggcaggt ccccgactgg
      841 gtcgtgatcc cgggcggcaa cctcggcaac gtctcggcgc tcggcgccgg gttcgagatg
      901 ctgctcgcgc tcggcctggt cgaccgccgg ccgcggatct gcgtcgcgca ggccgcccac
      961 gccggcccgc tgtacgcgag ctaccgcgcg ggctgggccg acctggtgcc ggtcaaggcg
     1021 cagaccacgc tggcgtcggc gatccagatc ggcaacccgg tgagcttcgg caaggcggtg
     1081 cgcgcgctgc gcgcgttcga cggcgtggtc gagatcgcga gcgaggccga gctcgccgac
     1141 gcgtcggctc aggccgatct cgatgggctg ttcacctgcc cacacaccgg cgtcgcgctg
     1201 gccgcgctgg ccaagctcgc cgcgcgcggc gagatccggc gcgatcagcg ggcggtggtg
     1261 atctcgaccg cgagcgggct caagttcgcc gacttcaagg tcggctatca cgaggcgaag
     1321 ctggccgggg tcgcgtcgcc gcgccaccgc aacgttccgg tcgagctgcc cgagcagtac
     1381 gcggcggtgc gcgaggcgct ggttcgcggg atcgccggct gacgccgcgc ggcggcgcgc
     1441 acaggtgaac ctgcgagtac gtctgacccg gcacgacgat tcgcccggtc gcgctgacct
     1501 ccgcagatcg gcggcggcag gcgctgacat cgcagcggcg ggtcgccgcg ggctcgcggt
     1561 tcacgtagcg cagggaccac acgtgaagcg ccggccacag cgccggcgag cgatctccgt
     1621 cggttgcgcg tggccctcgc attgcagcct cgcccggcgt gcgcgtcgca gggctgatca
     1681 tcgccgccgt gatcgtcgcc gtgtgggcga tgttccgcca tggtccggtg accgccgcca
     1741 acgccgaggt ggcagtggac cagccggtcg cccggctgca ggaagttcgc agcgtcgggc
     1801 tcgacggccg gcgcgtcctg tcggcgcggc tgcgcgaggt gatcgccacg cgcccgggcg
     1861 cccagctcga cgccgaccgg ctgcgcagcg accgcgacgt gatggagcgc gcgctggccg
     1921 acctcggcta cctcgccgcg cgggtcgagc cggcgaaggt cacgttcgac gccgccggcg
     1981 cggcctacgt gacgttcgcg gtcgaccagg gtcccatgtt ccatttacgg aacgtcgagg
     2041 tcacc
//
LOCUS       DASVOR010001730         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_514646,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001730 DASVOR010000000
VERSION     DASVOR010001730.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514646"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1370
                     /locus_tag="VF516_32210"
     CDS             <1..1370
                     /locus_tag="VF516_32210"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HEX8112454.1"
                     /translation="GSTTEDCLFLNVFAPNPFHDRGHGHGHGHDRDRGHDDDRDGDRD
                     RDRFGLHPVMVWIHGGALVTGESNDYDATRLVEQGDVIVVTINYRLGELGFLAHPALT
                     AESPDHASGNYGMLDQQEALRWVRRNILFFGGDPGRVTIFGESAGGLSVHSQLASPGS
                     AGLFQRAIVQSGAYQLTQPSLATAEATGTRFATAAGCTDQTAACLRGLSVAQILAAQP
                     GGTGGPGPNVDNKFLTRSVGAAFASGQFNQVPILEGANHDEWRLFVGITELQTGGPLP
                     AAAYPAAIQATLGIPAAVVPLFVAQYPLASFTSPSLALSALGTDGIFDCNARFVEQKV
                     SQFVPTFAYEFSDPGAPERFLPPVSFSYAAAHASEIQFVFDLPITVPAPALDADQQAL
                     AATMVSYWTTFARTGQPNSRGVPAFPAYQAASDTTQSLVPPQAAPETGFAAAHHCAFW
                     DSLRR"
     gene            complement(1393..1833)
                     /locus_tag="VF516_32215"
     CDS             complement(1393..1833)
                     /locus_tag="VF516_32215"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8112455.1"
                     /translation="MADTPPFGIRFTDPYRPLVVERAPTQDGVAHDDRVPVYRRAVEL
                     AARAHAVIERTETERFFLRDQLDRKSSLIPQLIAQGLAIAEMPARRALFRHARRALTD
                     CIAILDMLVERGSAPLDAVDGTRTLARALSEELIALTVEPPRAW"
     gene            1877..>2105
                     /locus_tag="VF516_32220"
     CDS             1877..>2105
                     /locus_tag="VF516_32220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112456.1"
                     /translation="MTHPYPTRALRGSTAPVQALRGRRRRSAASVGGSRCYLGTPVAA
                     PRDMPMAVDPAARSPARLACAFLAALAAVGCH"
BASE COUNT          273 a          813 c          724 g          295 t
ORIGIN      
        1 agggcagcac caccgaggac tgcctgttcc tcaacgtgtt cgcccccaat ccgttccacg
       61 accgcggcca tggccatggc cacggccacg atcgcgatcg cggccacgat gacgatcgcg
      121 acggcgatcg cgaccgcgac cgcttcgggc tgcacccggt gatggtgtgg atccacggcg
      181 gcgcgctggt caccggcgag agcaacgact acgacgcgac ccggctggtc gagcagggcg
      241 acgtcatcgt cgtcacgatc aactaccggc tcggcgagct cggcttcctc gcccaccccg
      301 cgctgaccgc ggagtcgccc gatcacgcgt cgggcaacta cggcatgctc gaccagcagg
      361 aggcgctgcg ctgggtgcgc cgcaacatcc tgttcttcgg cggcgacccc ggccgcgtga
      421 cgatcttcgg cgagtcggcc ggcgggctca gcgtccacag ccagctcgcc tcgcccggct
      481 cggccggcct gttccagcgc gcgatcgtcc agagcggcgc gtaccagctg acccagccga
      541 gcctggccac cgccgaggcc accggcacga ggttcgcgac cgccgcgggc tgcaccgacc
      601 agaccgccgc ctgcctgcgc ggcctgtcgg tcgcccagat cctcgccgcc cagcccggcg
      661 gcaccggcgg tcccggcccg aacgtcgaca acaagttcct gacccggtcg gtcggcgcgg
      721 cgttcgcgag cggccagttc aaccaggtgc cgatcctcga gggcgccaac cacgacgagt
      781 ggcggctgtt cgtcggcatc accgagctgc agaccggcgg gccgctgccc gccgcggcct
      841 acccggcggc gatccaggcc acgctcggca tcccggcggc ggtcgtcccg ctgttcgtgg
      901 cgcagtaccc gctggcgagc ttcaccagcc catcgctcgc gctgtccgcg ctcggcaccg
      961 acggcatctt cgactgcaac gcgcggttcg tcgagcagaa ggtgtcgcag ttcgtgccga
     1021 cgttcgccta cgagttcagc gatcccggcg cgcccgagcg gttcctgccg ccggtgtcgt
     1081 tctcgtacgc cgcggcgcac gcgtccgaga tccagttcgt gttcgacctg ccgatcacgg
     1141 tcccggcgcc ggcgctcgac gccgatcagc aggcgctggc ggcgacgatg gtgtcgtact
     1201 ggaccacgtt cgcgcgcacc ggacagccga actcgcgcgg cgtgccggcg ttccccgcct
     1261 accaggccgc cagcgacacg acgcagtcgc tggtcccgcc ccaggccgcg cccgagaccg
     1321 ggttcgccgc cgcgcaccac tgcgcgttct gggactcgct gcggcgctga gggttctccc
     1381 tcggcaccgg ggtcaccacg ctctgggcgg ctcgacggtc agcgcgatca gctcctcgct
     1441 cagcgcgcgc gccagcgtgc gggtgccgtc gaccgcgtcc agcggcgccg agccgcgctc
     1501 gaccagcatg tcgaggatcg cgatgcaatc ggtcagcgcc cggcgcgcgt gccggaacag
     1561 cgcgcggcgc gccggcatct cggcgatcgc caggccctgc gcgatcagct gcgggatcag
     1621 cgacgacttg cgatcgagct ggtcgcgcag gaagaaccgc tcggtctcgg tgcgctcgat
     1681 caccgcgtgg gcgcgggccg cgagctcgac cgcgcggcga tagaccggca cgcgatcatc
     1741 gtgcgcgacc ccgtcctggg tcggggcgcg ctcgacgacc agcggtcggt agggatcggt
     1801 gaaccggatc ccgaagggag gtgtgtccgc catcagatga tctcgtaaca cccggcccgc
     1861 cgcggttcat gcacggttga cccacccgta cccgacgcgc gcgctgcgcg gatccacggc
     1921 gccggtgcaa gcgctgcgcg gtcggcggcg gcgctccgcg gcgtccgtcg gtgggtcgcg
     1981 ctgctacctc ggcacgccgg ttgctgctcc tcgggatatg ccgatggccg tcgaccccgc
     2041 tgcccgcagc cccgcacggc tcgcctgcgc gttcctcgcg gcgctcgccg cggtcggctg
     2101 tcacc
//
LOCUS       DASVOR010001731         1536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_18934,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001731 DASVOR010000000
VERSION     DASVOR010001731.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1536
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18934"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1536
                     /locus_tag="VF516_32225"
     CDS             <1..>1536
                     /locus_tag="VF516_32225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8112457.1"
                     /translation="AEPEDGIQLQMVEVVLDGLRETAVEDLARLARLDAMAFELTERG
                     RRLEGELRRAFGDPAIDAIASRATIGEIERAFGDRAQDRAQARAEARIAVDALLMCDA
                     IAATVSEVGLGAGASEFGEPSVTRAPARPSAGSELFDLLFDDLGFGEMTSGGGGAVPI
                     EFDEGYDSGVVSTADVEAANLRRDDTAAARQLLAAEHQRIQNADHYTVLLIGRRARPS
                     EIEAAFAVRCALLDRDSPVIPDPRDRAKLDELRRVYQVARDTLLDEAARSAYDHEIAG
                     GELATAPPGIDAELAFRAAEELMARGQWAVAVGQIKTVITRSPGEADYHAALGWAEWR
                     AGGESPEAADTARPHLNQALAINPDHAAAHDYKGRISAALRSDDTEALFHLERALELD
                     PQRGDAIAAAEQLLIERGELRRYEKLLKQLLFRLRGKAPADEVKAWLRLAELYFDQLD
                     DAKAGAAAMANARQLTPDDPDVQRLSRRASGRIRRDTLEPIRAEWRDALADPQSGAVL
                     VKSA"
BASE COUNT          213 a          570 c          583 g          170 t
ORIGIN      
        1 gccgagcccg aggacggcat ccagctccag atggtcgagg tcgtgctcga cggcctgcgc
       61 gagaccgcgg tcgaggacct cgcgcggctg gcgcggctcg acgcgatggc gttcgagctc
      121 accgagcgcg gccggcggct cgagggcgag ctgcgccggg cgttcggcga tcccgcgatc
      181 gacgcgatcg cgagccgcgc gacgatcggc gagatcgagc gcgcgttcgg cgaccgcgca
      241 caggaccgcg cacaggcccg tgccgaggcc cgaatagccg tcgacgcgct gctgatgtgc
      301 gacgcgatcg cggcgaccgt ctccgaggtc gggctgggcg cgggcgcgtc cgagttcggc
      361 gagccgtcgg tgacgcgcgc gccggcccgg ccgtcggcgg gctccgagct gttcgacctc
      421 ttgttcgacg acctcgggtt cggcgagatg acgtcgggcg gcggcggcgc ggtgccgatc
      481 gagttcgacg aaggctacga ctccggcgtg gtgtcgaccg ccgacgtcga ggccgccaac
      541 ctgcggcgcg acgacaccgc cgcggcgcgc cagctgctcg ccgccgagca ccagcggatc
      601 cagaacgccg atcactacac ggtgctgctg atcggccgcc gcgccaggcc gagcgagatc
      661 gaggccgcgt tcgcggtgcg ctgcgcgctg ctcgatcgcg actcgccggt gatccccgac
      721 ccgcgcgacc gcgccaagct cgacgagctg cgccgggtct accaggtcgc gcgcgacacg
      781 ctgctcgacg aggccgcgcg gtccgcctac gatcacgaga tcgccggcgg ggagctcgcg
      841 acggcgccgc ccgggatcga cgccgagctc gcgttccggg ccgccgagga gctgatggcg
      901 cgcggccagt gggcggtcgc ggtcggccag atcaagaccg tgatcacgcg gtcgccgggc
      961 gaggccgact accacgccgc gctgggctgg gccgagtgga gggccggcgg agagagcccc
     1021 gaggccgcgg acaccgcgcg cccgcacctc aaccaggcgc tcgccatcaa ccccgatcac
     1081 gcggcggcgc acgactacaa gggccggatc agcgccgcgc tgcgcagcga cgacaccgag
     1141 gcgctgttcc acctcgagcg cgcgctcgag ctcgatccgc agcgcggcga cgcgatcgcg
     1201 gccgccgagc agctgctgat cgagcgcggc gagctgcggc gctacgagaa gctgctcaag
     1261 cagctgctgt tccggctgcg cggcaaggcg ccggccgacg aggtcaaggc gtggctgcgg
     1321 ctcgccgagc tgtacttcga ccagctcgac gacgccaagg ccggcgccgc cgcgatggcg
     1381 aacgcgcgcc agctcacccc cgacgacccc gacgtgcagc ggctgtcccg gcgcgcctcc
     1441 ggccggatcc ggcgcgacac gctcgagccg atccgcgccg agtggcgcga cgcgctggcc
     1501 gatccgcaga gcggcgcggt gctggtcaag agcgcc
//
LOCUS       DASVOR010001732         5399 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_163738,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001732 DASVOR010000000
VERSION     DASVOR010001732.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5399
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163738"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VF516_32230"
     CDS             complement(<1..190)
                     /locus_tag="VF516_32230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112458.1"
                     /translation="MIIERPYRIAGATGALAVMALIAVSARACTARGPATGAPVAATL
                     AVAAVPVAPSPAPASDRPL"
     gene            755..1753
                     /locus_tag="VF516_32235"
     CDS             755..1753
                     /locus_tag="VF516_32235"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8112459.1"
                     /translation="MGDLTAAEVDELAALGARLRDAGLHARGLAAWAGMARLSALPAR
                     LDALPAGAPTPAAAILALFIAGTEVERERLRTLPEAAIDQLAAWQLLDRNAGLLRARV
                     AILPLGAALLVCDRLDAPIEQALVCWPDDSSHHLATAIPPGRRTRWLDLGCGSAFAPL
                     ARPELAHQIAGVDINPRAVRHARLGAALSGLSQLTISPGDIDDLHEPADLVTCNAPMP
                     DPPGDGDRLRTTLPEVWRRTDPDFFTRLWPALARAVRPGGMAVVHAAAASILPALADV
                     PGERVVVTYTPDGVPGFAVAWWHPDAPPRLATARRALTPERPHLDPRDRDDALAPQ"
     gene            1928..2350
                     /locus_tag="VF516_32240"
     CDS             1928..2350
                     /locus_tag="VF516_32240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112460.1"
                     /translation="MDQLFTVGEYAKRNEIENKGRLRHMDKAAVYRFDGKHWCLLLNE
                     GNGFWNDRALPTGEVLMEVNPRPDKRRLNFDLEHASSRSQCTYMFYRKKGDEPETFEY
                     LGEVRCIDRQGNFLVFTRLVQPAAALPPAAARRAPPSP"
     gene            2490..2705
                     /locus_tag="VF516_32245"
     CDS             2490..2705
                     /locus_tag="VF516_32245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112461.1"
                     /translation="MSASSCSVTAIADPRVFAVAPVVLHGRPGARGRPDSGADAVGYG
                     GTYPVFWIDRFCASLMPPLPAKAGPIV"
     gene            3300..4061
                     /locus_tag="VF516_32250"
     CDS             3300..4061
                     /locus_tag="VF516_32250"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8112462.1"
                     /translation="MRVATRTAIRTKGRSEAEQLVLFKPRGGKRRGAGRPPKGTRAGS
                     PHKQRPFLHARYPVHVTLRVVGAVGNLRRRCVYQAIREATLTTARRENFRIVQLSIQR
                     THIHLLVEADDKHALARGMQGFEISAARHLNAAISKGKPGPRRRGVVFPDRYHAEIIT
                     SPRQARHALSYVANNWRRHQEDRRAPMSGWTIDWFSSAAMFTGWAEYGDEPFLWRGPP
                     TYDPLIVYQPKTWLLREGWKKAGSISCREVPSARK"
     gene            complement(4214..>5399)
                     /locus_tag="VF516_32255"
     CDS             complement(4214..>5399)
                     /locus_tag="VF516_32255"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEX8112463.1"
                     /translation="SATRGAGAGTARERSLRRLYRGVLLLCGVYFYLSIPILLLMIVA
                     AGGGAIYAFLAMGVIPIKLVLIIAIVVIATIGAILRGLFVRVAPASFGHRVDLDRQPR
                     LRALLDEVAGKVGTRPVDAVYLTPGTDMGVTERAGLWSSMRDSRVQRNLIMGIGLFDG
                     MTQLQLRSVLAHEYGHFRNADTAGGGFALAVRRSLFAIIIRLARSGAAGAYNPVWWFL
                     RAYHRIYLGVSQGASRLQEVLADRWAIEAYGTAAFVAGYRHVVSRSVQFDHQVDATIK
                     EVVDAHRPLPNLYQYRPQSGDAAERDVADAIHKEMTRAPTAYDSHPSPQQRIDGAQAL
                     AVEREAQPDDDASIWALFDDRDEIERTMTAEVRARIFASHGIDIAGAEQAAAGPASDG
                     PR"
BASE COUNT          871 a         1908 c         1810 g          810 t
ORIGIN      
        1 gcagcggccg gtcgctcgca ggcgccgggc tcggcgccac cggcaccgcg gcaacggcca
       61 gagtggcggc gactggtgcg cccgttgccg gaccacgggc ggtgcatgcc cgggcagaca
      121 cagcaatgag ggccatcacg gccagcgccc ccgtggctcc ggcgatccgg tacggacgtt
      181 cgatgatcat tcgacccaac gttggagccg acgcgatcat tcctggtcat cacggtctga
      241 tctgtcgtgt gttcgagatc cgcgcacagc ggcggcaacc cagacctcgg aagaccgcgt
      301 ggacggccct gtgactggct cgtcgaagct gaccaggtcg accaggacga cccactcaca
      361 gtgcatcgcc ccgcgcggac gctccttccg ttcatttgat ttcggtctgg atctccggag
      421 aagttcactc ggcgaagcag gcattgcgcc cgacgaatct ttgccggatc cgtcaaccgt
      481 caccggcgcg gaacttccgc ggccgaccac gctccgccgc tccttccccg ttgtttgatc
      541 cggtccggat ccccggagat gtccgctcgg cgcagcaggt actgccgccc gacgaatgtg
      601 acccgagcgt gagtgttcca gcacccgccg acgaatcctc actgggccgt gagcggtccc
      661 aggggaaact cccgggacgc ccgctccccg tgagcgcatc gagcgcatgg aaccggcatg
      721 gcgtgtgcgg tgttgcggag ataccctgcc cgacgtggga gacctcacgg ccgccgaggt
      781 ggacgagctc gcggcgcttg gagcgcggct ccgcgatgcc ggtctccacg cgcgcggtct
      841 cgcggcctgg gccggcatgg cgcggctgtc cgctctccct gcgcgactcg atgcgctgcc
      901 tgccggcgcg ccgaccccgg ccgccgccat actcgccctg ttcattgccg gcaccgaggt
      961 tgagcgcgag cgcctgcgca cgctgcctga agccgccatc gaccagctcg cggcatggca
     1021 gttgctcgac cgcaacgccg gcctgctgcg cgcgcgcgtc gcgatcctgc cgctcggcgc
     1081 tgcgctgctg gtctgcgatc gcctcgatgc gccgatcgag caggccctcg tgtgctggcc
     1141 agacgactcc agccaccacc tcgccaccgc gattccaccc gggcgccgca cccgctggct
     1201 cgacctcggc tgcggctcgg cgttcgcccc cctggcgcgg cccgagctcg cgcaccagat
     1261 cgctggcgtc gacatcaacc cgcgcgccgt ccgccacgca cggctgggcg ccgcgctgtc
     1321 cggcctgtcc cagctcacga tctcgcccgg cgacatcgac gacctacacg agcccgcgga
     1381 cctcgtgacc tgcaacgccc ccatgccgga cccgcccggc gacggtgacc gcctgcgtac
     1441 cactctgccc gaggtctggc ggcgcaccga tcctgacttc ttcacccggc tgtggcccgc
     1501 gctcgcgcgc gccgtgcgcc ccggcggcat ggccgtcgtc cacgccgccg cggcctcgat
     1561 cctaccggcc ctcgccgatg tccccggcga gcgcgtcgtc gtcacctaca cccccgacgg
     1621 cgtgcccggc ttcgcggtcg cctggtggca ccccgacgca ccgccacggc tcgccaccgc
     1681 gcgccgcgcg ctcacacccg agcgaccgca cctcgatccg cgcgaccgcg acgacgccct
     1741 cgctccgcaa tgatcgggcc gccccccagc acgacgaacc taatttcgag cagttgagaa
     1801 gcttgactct gtcatgctta cacacacgat atcaactact tggacagccg gccgcgtggg
     1861 cagactgtcc cagcgacgtc gtgggcaggg taaacatggt cgcttccgag ccaagaagga
     1921 cacatccatg gaccagctgt tcacggtcgg cgaatacgcg aagcgcaacg agatcgaaaa
     1981 caagggccga cttcggcaca tggacaaggc tgcggtctat cgctttgacg gcaagcactg
     2041 gtgcctcctt ctcaacgaag gcaatggctt ctggaacgat cgcgccttgc cgaccggcga
     2101 ggtgctgatg gaggtgaacc cgaggccgga caagagacgg ttgaacttcg acctcgaaca
     2161 tgcgtcaagc cggagtcagt gcacgtacat gttctatcgc aagaaaggcg atgaacccga
     2221 gacctttgaa tacctcggag aggtcaggtg catagatagg caagggaact ttttggtgtt
     2281 cacacggctc gtccagcccg cagccgctct cccgcccgct gcagctcggc gtgcgccgcc
     2341 ttctccgtga cgcccaggac gagcccacgc tgcgctcggt gagcccgtcc gggtcccgtc
     2401 cgcgatgtcg agcgcgcagc cgtgctcagc gtcaccgcgt gggtgatgca ggcgagtggg
     2461 caggcgcggc ggggtgcggc ccagtagacg tgagcgcgtc gagctgctcg gtcaccgcga
     2521 tcgccgaccc gcgcgtgttc gcggtcgcgc cggtagtgct tcacggtcgg cctggcgctc
     2581 gcggtcggcc ggacagcggc gccgacgccg tgggctatgg cggcacctac ccggtgttct
     2641 ggatcgaccg cttctgcgcc tcgctcatgc caccgctgcc tgcaaaggcc ggtcccatcg
     2701 tctgaagctc cgtcggggaa cgacctgcaa cctgcctgtt cgacgggtct agataccgag
     2761 gagctcggcg tgatgcctga gcgccgagcg caccctctcc agagatcgaa aaaaaacaaa
     2821 caatggaagg gagcgtccgc gcagagcgat gcactgtgag tgggtcgtcc tggttggcct
     2881 gatcagcttc gacgagccag tcacagggcc gtccacacgg tctcccgagg tccccggctg
     2941 cggccaccgt gcagcgatca tcgagtgcca tccgtcgagc ttgagcaccc attgctcgat
     3001 tgtgggcgca cgctgcgcgc gtcacccgcc cgctcgacga ggacagcgcg taccgggcga
     3061 gcagcacacg cagaacccag cgacatcggt gaagccgcgc ggatgggcgt cgggcagcga
     3121 tgctcgctga gcgtaccggg aagcccatcg gacaccgcca agacaggacg ctgctgcacg
     3181 aatactggcg aatgtgcacc catgcgcgac aatgatgtct gttgtcgatt acattgctca
     3241 tactatgaat tacggagaaa tcaattgact gaatggcgat ctggatttag caaggatgca
     3301 tgagggtcgc gacgcgaacc gccatccgca ccaaggggcg ctccgaggcg gagcagctgg
     3361 tgctgttcaa gccgcgcggt ggcaagcggc gcggggccgg tcgcccgccg aagggcacgc
     3421 gggccggttc gccgcacaag cagcggccgt tccttcacgc tcgctacccc gtccatgtca
     3481 cgctgcgggt cgtcggtgcc gtcggcaacc tgcgccggcg ctgcgtgtac caggcgatcc
     3541 gcgaggccac gctcaccacg gcgcggcgcg agaacttccg catcgtccag ctcagcatcc
     3601 agcgcacaca catccacctg ctggtcgagg ccgacgacaa gcacgcgctg gccaggggga
     3661 tgcagggctt cgagatctcg gctgcaaggc acctcaacgc ggcgatcagc aagggcaagc
     3721 cgggcccgcg gcggcgaggc gtggtgttcc ccgaccgcta tcacgccgag atcatcacct
     3781 cgccacgcca ggcgcgccac gcgctgtcct atgtcgccaa caactggcgc cgccaccagg
     3841 aggaccgccg ggcaccgatg tcgggctgga cgatcgactg gttttcgagc gcggcgatgt
     3901 tcaccggctg ggcggagtac ggcgacgagc cgttcctgtg gaggggccct ccgacctacg
     3961 atccgctcat cgtgtaccag ccgaaaacgt ggctgttgcg agaaggttgg aagaaagccg
     4021 gatcgatctc gtgccgcgaa gtcccgtcgg cgagaaaatg atcctgagga tcatcggctc
     4081 gctccttggg cggcttccca tcgcgaggtc ttgcggcgag gagcgcaccg tgtggtcggc
     4141 gctatcacgc ggaccggcga acaagcgcgc caccgggcgg tgggcccgcc ccgcctggcc
     4201 gacggcggga cgatcagcgc gggccgtcgc tcgccgggcc tgcggcagcc tgctcggcgc
     4261 cggcgatgtc gatcccgtgg ctcgcgaaga tccgggcgcg cacctcggcc gtcatcgtgc
     4321 gctcgatctc gtcccggtcg tcgaacagcg cccagatcga tgcatcgtcg tccggctgcg
     4381 cctcgcgctc gaccgcgagc gcctgcgcac cgtcgatccg ctgctgcggg gaggggtggc
     4441 tgtcgtaggc ggtcggcgcg cgcgtcatct ccttgtggat cgcgtcggcg acatcgcgct
     4501 ccgcggcgtc gccggattgc gggcgatact gatacaggtt ggggagcggg cggtgtgcat
     4561 cgaccacctc cttgatcgtc gcgtcgacct ggtggtcgaa ctgcaccgag cgggagacca
     4621 cgtggcgata ccccgcgacg aaggccgccg tcccgtaggc ctcgatcgcc catcggtcgg
     4681 cgagcacctc ttgcagccgc gaggcgcctt gcgacacgcc gaggtagatc cggtgatacg
     4741 cgcgcaggaa ccaccacacc gggttgtacg cgccggccgc gcccgagcga gcgagccgga
     4801 tgatgatggc gaacagcgag cggcgaaccg cgagcgcgaa cccgccgccg gcggtatccg
     4861 cgttgcgaaa gtgcccgtac tcgtgcgcga gcaccgagcg cagctggagc tgggtcatgc
     4921 cgtcgaacag gccgatgccc atgatcaggt tgcgctgaac gcggctgtcg cgcatcgacg
     4981 accacaggcc ggctcgctcg gtcaccccca tgtcggtgcc cggcgtgagg tagaccgcgt
     5041 cgaccggccg cgtgccgacc ttgccggcga cctcgtcgag cagcgcgcgg agccgcggct
     5101 ggcgatcgag atcgacccgg tggccgaagg aggcgggcgc gacgcggacg aacagaccgc
     5161 ggaggatcgc gccgatggtg gcgatcacga cgatggcgat gatcaggacg agcttgatcg
     5221 ggatcacccc catcgcgagg aaggcgtaga tcgccccgcc gcctgcggcg acgatcatga
     5281 gcaggaggat gggaatcgac aggtagaagt acacgccgca cagcagcagg acgcccctgt
     5341 acagccggcg caggctgcgc tcgcgggcgg tgccggcgcc ggcgccgcgc gtggcgctg
//
LOCUS       DASVOR010001733         1820 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_338947,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001733 DASVOR010000000
VERSION     DASVOR010001733.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1820
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338947"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VF516_32260"
     CDS             <1..931
                     /locus_tag="VF516_32260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8112464.1"
                     /translation="KQRDHSSLDRCCPKRHMSEWKTRITKVQVVKPAKEAGEACLVLI
                     YPPGPDMGKRFPLLRSEIVLGRGADCDIQVDRDSVSRRHARVYRNGETWSVEDLQSTN
                     GSYVNDVPVTRSVLRDADFLKIGAAIFKFLSGTGVEASYYEEIYRMTIVDALTGAYNK
                     RYFLEFLEREIARCARYRRPLSLLMFDIDHFKAINDKHGHLTGDYVLREMSRRLLSRI
                     RREELLARYGGEEFAAVLPETDLTGARTFGEQVRRIVGDQMFEYEGDTFPVTISVGVA
                     TVEGEDIDVSSFIKQADDLLFRAKREGRNRVLG"
     gene            1176..>1820
                     /locus_tag="VF516_32265"
     CDS             1176..>1820
                     /locus_tag="VF516_32265"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEX8112465.1"
                     /translation="MLRAHADPLLWTLGLFLAVVIVAALVNRFRPAQRPRLRRLVTVF
                     ALFATATLAGIGFQTAGLASWSDGTLLAAQILQAFTLISLSATLVIGVVLPAVGIELP
                     MIVSDLIVGTGYVVATLVILAHHGVDPTGALVSGAVVSAVLAISLQSTLGNIVGGVAL
                     QLDGSIREGDWIQLENGKQGRVRSVRWRHTVIETRDWSTVIVPNAQLLATTITIL"
BASE COUNT          269 a          612 c          631 g          308 t
ORIGIN      
        1 aaagcagcgc gatcacagca gtttggatcg ctgctgcccg aagcggcaca tgagcgagtg
       61 gaagacacgc atcaccaagg ttcaggtcgt caagccggcg aaggaagcgg gcgaagcgtg
      121 cctcgtactc atctatccgc ccggtcctga catgggcaag cggtttccgc tgctgcgcag
      181 cgagatcgtg ctcggccgcg gcgccgattg cgatatccag gtcgatcgcg actcggtgtc
      241 gcgccgccac gcgcgggtct accgcaacgg cgaaacctgg tcggtcgagg atctgcagtc
      301 gaccaatggc tcgtacgtca acgacgtgcc ggtgacgcgc tcggtgctgc gcgacgccga
      361 cttcctcaag atcggcgcgg cgatcttcaa gttcctgtcg gggaccgggg tcgaggcgag
      421 ctactacgaa gagatctacc ggatgacgat cgtggacgcc ctgaccggcg cctacaacaa
      481 gcgctacttc ctggagttcc tcgagcgcga gatcgcccgg tgtgcgcggt atcgccggcc
      541 gctgtcgctg ctgatgttcg acatcgatca cttcaaggcg atcaacgaca agcatggcca
      601 cctgaccggc gactacgtgc tgcgcgagat gtcgcgccgg ctcttgtccc ggatccgccg
      661 cgaggagctg ctcgcgcgct acggcggcga ggagttcgcg gcggtgctgc ccgagaccga
      721 tctgaccggc gcgcggacgt tcggcgagca ggtccgccgg atcgtcggcg atcagatgtt
      781 cgagtacgag ggcgacacgt tcccggtcac gatctcggtc ggcgttgcga cggtcgaggg
      841 cgaggacatc gacgtgtcgt cgttcatcaa gcaggcggat gacctgctgt tccgggccaa
      901 gcgcgagggc cggaaccggg tcctgggata ggagcctgta gtgcgtggcg cccgggagaa
      961 cgcgtgcacg cggctcgccg cgttgacgtc tctgcggtgc cgcccgccct ctggcacgac
     1021 gagctcctcg atcgtccgga tcaccgcggc acaccgcccc ggtcggttga tcaggcgccc
     1081 ttcccgtctc cgctcgccgg caagcggcga ttgacgcttc gcgcgtcgcg tcgcgcggat
     1141 acctgtgggc gcgggcgccg gttataaatg aaggggtgct gcgcgcgcat gctgacccgc
     1201 tgctctggac gctcgggctg ttcctcgcgg tcgtgatcgt cgcggcgctg gtcaaccggt
     1261 ttcgcccggc gcagcgcccg cggctgcgcc ggctggtcac cgtgttcgcg ctgttcgcga
     1321 ccgcgaccct ggccgggatc gggttccaga ccgccgggct ggcgagctgg agcgacggca
     1381 cgctgctcgc cgcccagatc ctccaggcgt tcaccctgat cagcctgtcg gcgacgctgg
     1441 tgatcggggt cgtgctgccg gcggtcggca tcgagctgcc gatgatcgtc agcgatctga
     1501 tcgtcggcac cggctacgtc gtggccaccc tcgtgatcct ggcgcaccac ggggtcgacc
     1561 cgaccggcgc gctggtgtcc ggtgccgtgg tcagcgcggt gctcgcgatc tcgctgcaga
     1621 gcacgctcgg taacatcgtc ggcggcgtcg cgctccagct cgacggctcg atccgcgagg
     1681 gcgactggat ccagctcgag aacggcaagc agggccgggt ccgctcggtg cgctggcgcc
     1741 acaccgtgat cgagacccgc gactggtcga cggtcatcgt ccccaacgcc cagctgctcg
     1801 ccaccacgat cacgatcctc
//
LOCUS       DASVOR010001734         4452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_514688,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001734 DASVOR010000000
VERSION     DASVOR010001734.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514688"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(674..1525)
                     /locus_tag="VF516_32270"
     CDS             complement(674..1525)
                     /locus_tag="VF516_32270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112466.1"
                     /translation="MSDPGRRAAAARLWFGQEPASIDAAVAVDSSASDAAAIDATAPD
                     ADLGPHLVFVTSTTHDCALGGLTGADAICQARARAANLPGSYMAWLADANASPATRMT
                     HHVGVYQLVTGAAIAQGWNDLTDGNLAGKIDRTEGGVELGGVGCNAPSPTCHFICEGG
                     EVWSNVDGAGNRRNVADCTGWTTLGNATAGNDGKTDVTWTAGIAPPSPRAARCRSSAS
                     SSERPGSSWDDLLLSRPPEFRAGSAPKAAFQRTHKRACRCHRLWARGLCTRLDPVRDP
                     AGHRTGT"
     gene            1681..2922
                     /locus_tag="VF516_32275"
     CDS             1681..2922
                     /locus_tag="VF516_32275"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8112467.1"
                     /translation="MRSSNLLLVIACLLAAGLPRPAAAQPAAARRIVIAAGTLLDGKG
                     HVVKNTRIVVDGAKIVALDPKAGPVDYDLHTLTVMPGWIDAHDHLTWSFGADGKNAGA
                     DRTTQEAAYASAANARVTLLAGVTTIQSVGSPTDVPLRDAIARGLVPGPRLLTAVEPL
                     EGKGPKTGTPDEIRAFIRKQKQAGADVIKIFASESIRTGGAMTLSQAQLDAACDEAKK
                     QGLRTLVHAYKQAVRAAALAGCTQVEHGSLATDEDLKVLADKGTYFDPQAGLVIENYL
                     QNKDKYLGTEGYVEEGFAAMQKILPLNHDMVKRASKIPGLKIVFGTDAVAGAHGRNAE
                     EFIDRVRDAGVDPMTALVSANALAAEAMGLADQIGSIAPGLQADIIALDGDPVKDITA
                     VRRVVFVMKGGVVYKTPSGAR"
     gene            2924..3754
                     /locus_tag="VF516_32280"
     CDS             2924..3754
                     /locus_tag="VF516_32280"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8112468.1"
                     /translation="MSTAIPDALHPASGDGNGDDAILVQRDGAIATVVLNRPDKLNAL
                     TRRMWIRLGEVFARLDADDRLRCIVIRGAGTRAFAPGNDIAEFAAERSNVEQARAYGE
                     DMRRAIESIGGCRHPIVAQIHGICVGGGLEIAGLADLRICGESSRFGVPINKLGLVMA
                     YAEIGALIALVGEPTALEMLLEGRIFDAREAHAKGLVTRVVADDQVEAEARAAAQRIA
                     DGAPLVARWHKKFARRLRDPRPLTAAEYDEGFACFGTEDFQIGYRAFLAKQRPEFTGK
                     "
     gene            3751..>4452
                     /locus_tag="VF516_32285"
     CDS             3751..>4452
                     /locus_tag="VF516_32285"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEX8112469.1"
                     /translation="MTGALHGIKVVELAQIMAGPTCGLMLADLGADVIKIEKLPAGDD
                     TRAYREPAIHGEPASFMILNRNKRSLAVDLKTPGGLEVVRRLVAGADVLIENYRKDTL
                     DRLGLGYDAVSRLNPRLVYCAISGYGRTGPYADKGGFDLIAQGFAGIMSITGEPGGPP
                     AKSGTSVADINAGILAALGIVSALVARATTGRGQLVETSLMEAAVQQTYWQAALFFAT
                     GINAGPTGSAHILTAP"
BASE COUNT          692 a         1590 c         1557 g          613 t
ORIGIN      
        1 ctcgctgccg cccggccggg tccgccgccg ccgcgatcgc cgagcgctcg accaagtcga
       61 cgtcggcgac gcgcccgcca gcgcgagcag ctcgccgtcg gtcgccgccg caatcgccga
      121 gctcgggacc ccaggagcaa cggcgcggga gttacggtag gtgacgcagg cgagtcatga
      181 ggactcaatt cacaatacgc gcctaggcag gatcatcgtc tgaaatcgct tagatatact
      241 ggcaaatcct gccggcgatt tgaacctact catcaccaat ttggtgtgcc ggtcgaggtc
      301 acgagcgtgc tcggtaggct tcacgtcgac caccgcaggg ccgcttgccc ccggcgattc
      361 tgcccgaaga catcggtagc cagcacggcg ctcgcggtcg gacgagcgct cggcggcgcc
      421 cgccggggcc gctggtgcgc caagggccca ccaggcgctc gaacgacgcg caccggggcg
      481 ccacgcacgc gctcgggcgg ggccgcgtgc aggcgtggtc cctctgctag ctcagccatc
      541 ggcgataact ccatttcgcc atttcttcgc cgggcatcac ggatccaggg gggcagaacc
      601 gactccggtg gtacatggcc cggctccaag tgccggagag agctgcaatg cgttgccggc
      661 aaaaggttct ctctcaggtg cctgtccggt gtccggccgg atctcggacg ggatccagcc
      721 gtgtgcagag cccccgggcc caaagcctgt ggcagcgaca agcgcgcttg tgcgtccgct
      781 ggaaggccgc cttcggcgcg cttcccgcac gaaattcagg aggtcgagag aggagaagat
      841 cgtcccaaga cgagcctggt cgctcactgc tggacgcaga agatcggcag cgggctgccc
      901 gcggcgatgg gggtgcaatc cccgcggtcc aggtcacgtc ggtcttgccg tcgttgcccg
      961 cggtggcgtt gcccagcgtg gtccatcccg tgcagtcagc gacgttccgg cggttgccag
     1021 cgccgtcgac gttgctccac acctcgccgc cttcgcagat gaagtggcag gtcggcgagg
     1081 gagcgttgca cccgacgccg cccagctcca cgccgccctc ggtgcgatcg atcttgccgg
     1141 cgaggttgcc gtccgtgaga tcgttccagc cctgcgcgat cgcggcgccg gtcaccagct
     1201 gatagacgcc cacgtggtgc gtcatgcggg tcgccggcga ggcgttcgcg tcggcgagcc
     1261 acgccatgta gctgccgggt agattggccg cccgcgcgcg tgcctggcag atcgcgtcgg
     1321 cgccggtgag cccaccgagc gcgcagtcgt gcgtcgtgct ggtgacgaac accaggtggg
     1381 ggccgaggtc ggcatcgggg gcggtcgcgt cgatcgcggc ggcatcgctc gcgctgctgt
     1441 cgaccgcgac ggcggcgtcg atggaagcag gctcttgacc gaaccacaac cgagcagcag
     1501 cagcccggcg accgggatcg ctcattcgaa aagtctatcg caacccccgc cagcgcccgc
     1561 gtgaccgatg tccggtccgc ctgcggcgtg ccgggctcga tggccgcgca aggaccgacg
     1621 ggtccttgac cgggcgttcg tgatatgaag ccggccatcg atcctgtagg agggtccgcg
     1681 atgcgatctt cgaacctgct cctcgtcatc gcctgcttgc tcgctgccgg cctgccgcgc
     1741 ccggccgccg cccagcccgc cgcggcccgg cgcatcgtga tcgccgccgg cacgctgctc
     1801 gacggcaagg gccacgtcgt caagaacaca cggatcgtgg tcgacggcgc caagatcgtc
     1861 gcgctcgacc ccaaggccgg cccggtcgac tacgacctgc acaccctgac cgtgatgccg
     1921 ggctggatcg acgcccacga tcacctgacc tggagcttcg gcgccgacgg caagaacgcc
     1981 ggcgccgatc gaaccacgca ggaggccgcg tacgcctcgg cggcgaacgc ccgggtgacc
     2041 ctgctcgccg gggtcacgac gatccagagc gtcgggtcgc cgaccgacgt cccgctgcgc
     2101 gacgcgatcg cgaggggcct cgtgccgggg ccgcgcctgc tcaccgcggt cgagccgctg
     2161 gagggcaagg gccccaagac cggcacgccc gacgagatcc gcgcgttcat ccgcaagcag
     2221 aagcaggccg gcgccgatgt catcaagatc ttcgcgtcgg agagcatccg cacgggcggc
     2281 gcgatgacgc tgtcgcaggc ccagctcgac gccgcgtgcg acgaggcgaa gaagcagggg
     2341 ctgcgcacgc tggtccacgc ttacaagcag gcggtgcgcg cggccgcgct ggccggatgc
     2401 acccaggtcg agcacggctc gctcgccacc gacgaggatc tgaaggtgct ggccgacaag
     2461 ggcacgtact tcgacccgca ggccggcctg gtgatcgaga actacctgca gaacaaggac
     2521 aagtacctcg gcaccgaggg ctacgtcgaa gaagggttcg cggcgatgca gaagatcctg
     2581 ccgctcaacc acgacatggt gaagcgcgcg tcgaagatcc ccggcctcaa gatcgtgttc
     2641 ggcaccgacg cggtcgccgg cgcgcacggc cgcaacgccg aggagttcat cgaccgggtc
     2701 cgcgacgccg gcgtcgatcc gatgaccgcg ctggtgtccg ccaatgcgct ggccgccgag
     2761 gcgatgggcc tggccgatca gatcggctcg atcgcgccgg ggctgcaggc cgacatcatc
     2821 gcgctcgacg gcgatccggt gaaggacatc accgcggtgc gccgcgtcgt gttcgtgatg
     2881 aagggcggcg tcgtgtacaa gaccccgagc ggtgcgcgct gacatgtcca ccgccatacc
     2941 ggacgcgctt caccccgcga gcggcgacgg gaacggcgac gacgccatcc tcgtgcagcg
     3001 cgacggcgcg atcgcgaccg tcgtgctcaa ccggccggac aagctcaacg cgctgacccg
     3061 gcggatgtgg atccggctcg gcgaggtgtt cgcgcggctc gacgccgacg accggctgcg
     3121 ctgcatcgtg atccgcggcg ccggcaccag ggcgttcgcg ccgggcaacg acatcgccga
     3181 gttcgccgcg gagcggtcca atgtcgagca ggcgcgggcc tacggcgagg acatgcggcg
     3241 cgcgatcgag tcgatcggtg gctgccgcca cccgatcgtc gcccagatcc acgggatctg
     3301 cgtcggtggc ggcctcgaga tcgccgggct cgccgacctc cggatctgcg gcgagtcgag
     3361 ccggttcggc gtgccgatca acaagctcgg cctggtgatg gcgtacgccg agatcggcgc
     3421 gctgatcgcg ctggtcggtg agccgaccgc gctcgagatg ctgctcgagg gccggatctt
     3481 cgacgcgcgc gaggcgcacg ccaagggcct ggtgacccgg gtggtcgccg acgaccaggt
     3541 cgaggccgag gcccgggccg ccgcgcagcg gatcgccgac ggcgcgccgc tggtggcgcg
     3601 ctggcacaag aagttcgcgc gccggctgcg cgacccgcgg ccgctcacgg ccgccgagta
     3661 cgacgagggc ttcgcgtgct tcggcaccga ggacttccag atcggctatc gcgcgttcct
     3721 cgccaagcag cggccggagt tcaccggcaa atgacgggcg cactgcacgg catcaaggtc
     3781 gtcgagctcg cccagatcat ggccggcccg acctgcggcc tgatgctggc cgacctcggc
     3841 gcggacgtga tcaagatcga gaagctgccc gccggcgacg acacccgcgc ctaccgcgag
     3901 ccggcgatcc acggcgagcc ggcgtcgttc atgatcctca accgcaacaa gcgcagcctc
     3961 gcggtcgacc tcaagacccc cggcggcctg gaggtcgtcc ggcggctggt cgccggcgcc
     4021 gacgtcctga tcgagaacta ccgcaaggac acgctcgacc ggctcggcct cggctacgac
     4081 gcggtgagcc ggctcaaccc gcggctggtg tactgcgcca tctcgggcta cggccgcacc
     4141 ggcccgtacg ccgacaaggg cggcttcgat ctgatcgcgc agggcttcgc cgggatcatg
     4201 agcatcaccg gcgagcccgg cggcccgccc gccaagagcg gcacgtcggt cgccgacatc
     4261 aacgccggca tcctcgccgc gctcggcatc gtctccgcgc tggtcgcgcg cgccaccacc
     4321 ggccggggcc agctggtcga gacctcgctg atggaggccg cggtccagca gacctactgg
     4381 caggcggcgc tgttcttcgc gaccggcatc aacgccgggc cgaccggctc ggcccacatc
     4441 ctcaccgcgc cg
//
LOCUS       DASVOR010001735        15796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_368649,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001735 DASVOR010000000
VERSION     DASVOR010001735.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15796
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368649"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(355..1761)
                     /locus_tag="VF516_32290"
     CDS             complement(355..1761)
                     /locus_tag="VF516_32290"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8112470.1"
                     /translation="MLGIAALLCPGAARADVDAATRKLAHDLFQQLIEIDTTGSTGSV
                     TAASEAMAQRLRAAGFPESDIHVVGPDERKKNLVVRLRGTGKRKPLLLIGHLDVVEAR
                     REDWTTNPFQLVERDGYYYGRGTQDMKDGDAILLTTLIRFKKEGYRPDRDIILALTAD
                     EESGSSNGVAWLFKHHRELVDAEFALSNDGPDVVMEHGKALYVEIDASEKVYADFQLT
                     VTSPGGHSSMPVPDNAIYHLTGGLDRLSRYEFPFELSNVTRGYYERQATLETGSRAAD
                     MRAILKVPPDPAAIARLAGDPLDHAVTHTTCVATALEAGHANNALPQTARANVNCRIL
                     PGHSAEQVRKALIGVLADPRITVRYVADDGRVLDTAESKHGFTPAPLRQDVMRSLEQV
                     AASMWPGVPVIPMLAVGASDAVHVSAAGIPVYQVNGEAMDRDDLRAHGKDERTPVESF
                     YRGVDFHYAFLKTLMAQD"
     gene            complement(1776..3509)
                     /locus_tag="VF516_32295"
     CDS             complement(1776..3509)
                     /locus_tag="VF516_32295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8112471.1"
                     /translation="MSGQPGFLAELFLGRLRCDLLQPFPEQDPDERRQGDAVVAELSE
                     FLRRRVDPTEVDETRRLPDGLFGQLQERGYLRLTVDRPLGGLALSPYNTFRAIQATAS
                     WSPPVALAMAIHNGLGSAAYLPFLPAGPLRDLITARVAQGIVSGGADTEPAGAANRLR
                     TTTATPIENGAAYLLNGEKIFIGNGPEADLLDVSATLREGGREQVRLFFVDTRSPGFQ
                     VAGRHEFLGLRGASNARLVLRDVRVPREHMLGGDEDEWRLSPQISLMAATARIYIIGA
                     PSLAIAKLCAHWSRDFLCRRNVDGRPLGEYDQIQRMVALSLAEAFAIDSVVIWALCGA
                     RRADTRYERVAAKNITSLTSWRIIDRTMSLLGAEGYETGPSKARRGAPGLPVERFLRD
                     ARGLRIAGGVDFQVDFWAAQSGLLACYYPSDRPGSGSTRPGALPPAETRPAMPGLDAR
                     NADHLEYTGSQVTRLARTCVELVQRYPDPEALYARESIVMGLGQLTRELFTMAVVLAR
                     AAHLAQQGQVEARDLADVYCGAARHRVEGAWRRLSTGPEPDHARVSQRWLSRAAHGLL
                     TEDLILTGDPA"
     gene            complement(3506..4309)
                     /locus_tag="VF516_32300"
     CDS             complement(3506..4309)
                     /locus_tag="VF516_32300"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8112472.1"
                     /translation="MSHPRPAASHPDAGSAWLACRQRRPDAAVRLYCFPHSGGSAGEY
                     VRWSDGLPGIEVWGVQLPGRGSRVAEECYLRVDALVEAIVDAVAFQPPFALFGHSLGA
                     VVAFETARALRDRGGPKPEALIVSAYHAPHLPSTSPEVHRLPDEQLWPIVERQFGELS
                     PELHDDPDFRQLILAYHRADFEMIETYRHAEGPPLSVPLTVVGGTEDYPEDMLRAWRR
                     HTTGPFALQRFTGDHFYLRKQRDGLLRFLSSTLASRSRSSVAAPRRTAG"
     gene            complement(4323..5681)
                     /locus_tag="VF516_32305"
     CDS             complement(4323..5681)
                     /locus_tag="VF516_32305"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8112473.1"
                     /translation="MSAVTPLKRPSNLWRHRGFMMLWSAQSISLLGSQVSLLAIPLTA
                     ILLLNASTMQIGLLTAAGYLPVLLVGLPAGVWVDRMRRRPILIVADLGRVAILLIIPI
                     AYALDGLRMWMLYPIAFSAGILTLFFDVAHQSYLPSLVPREQLTEGNAKLEFSYSGAQ
                     LAGPGLGGALVQFLTAPIAIVADALSYLLSAVLLFAIRTPEPAPARPEGEEAGIGKQI
                     REGLRYVLGHSLLRPIALTTGIGNLFDLFGMVAAILTLYAVRELHLTPVALGGVLAVA
                     NAGALLGATLNGRLIRRFGVGPMIAVSSVIPGLAVLLLPLATPETAIPVLSIALGLAG
                     AAISIYNINQITLRQTVTPDAMQGRMNATIRVLIWGTLPIGTFLGGLLGGTIGLRPTL
                     VIAGIGSALASLPLMLSPVRTLRELPKAPGDAVAEPSPGDAVAEPAPGGAVAEEVMTA
                     QG"
     gene            complement(5678..5890)
                     /locus_tag="VF516_32310"
     CDS             complement(5678..5890)
                     /locus_tag="VF516_32310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbtH family protein"
                     /protein_id="HEX8112474.1"
                     /translation="MFEDDDNRPYCVVRNEEEQYSIWPADRAPPAGWTAQGVAGTKAE
                     CLSHIERTWKDLRPRSLRTAERMESR"
     gene            complement(5895..8816)
                     /locus_tag="VF516_32315"
     CDS             complement(5895..8816)
                     /locus_tag="VF516_32315"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8112475.1"
                     /translation="MACRFPGAETPDELWNNLVGGVESISPLGPLDPVTGHQAVCGVV
                     ENADRFDAGFFGYAPREALLLDPQHRVFLECSWAALEDAGVDPARYPGAIGVYAGSTH
                     TQYPSVLMSQRRRLGAVSEFQIRIATGMDFLTTRVAYKLGLTGPAITVQTGCSTSLVA
                     IHMAAQALLAGDCDMALAGGVTLHVPADPGEYMEGGILSRDGHCRAFDAAANGTVGGD
                     GAGVVVLRRLADALADGDPIRAVVRGSAVNNDGFDKIGYTAPSIEGQARVIRAAQLIA
                     EVAPETITYIEAHGTGTPLGDPIEIAALTKAFRQGTDRRGFCRIGSIKTNIGHTDAAA
                     GVAGFIKTVLALQHRELPPSLHFRAPNPEIDLAGSPFVVPTCRTPWDAAGTPRRAGVS
                     SLGIGGTNAHVVLEEWPEPPADPGPPHHLLVLSARTRSALDAATGRLAEHLRRNPDLA
                     LADAAWTLQVGRQEQRYRRIVVAERAADAAAALAEASSAGMGAVAEAGRPSVVFLFPG
                     QGGQHVGMGRDLYQHQPAFAAAIDECAELASAPLGLDLRTVLYPTGDPERARQQLATM
                     ALGQVTVFVVEYALAQLLAAWGVQPGLVLGHSLGAYAAACTAGVFSLPDALRLVVERG
                     RLLDRLAEGAMLAVRLPEQEVRELLGEELSLAAINGVNQCVVSGPAARVGALAAALTA
                     RGIDARRLHIPAGAHSTLADPILAEFEARVSQLDLRAPSVPWVSDTTGTWATADGPTR
                     PDYWTAHLRQPVRFADALATVLGTPGQVLVEVGPGRTLITLARQHPRFGDTLAVPMLS
                     HAAESTSDLAVSLAAAGRLWSAGVPLDWTRLHEGERRRRVALPTYPFQGQRYLVEAPA
                     EAQVDRAGAEPILLPDLPREDDASPALPPASGSEREQAVCGIFAEILGLARVHPGDNF
                     FQLGGDSLIATQLTAWIRRSYQTSLSLKEVFTAPTPQALAALLDRRLSTPTPSLSREE
                     A"
     gene            complement(8870..13951)
                     /locus_tag="VF516_32320"
     CDS             complement(8870..13951)
                     /locus_tag="VF516_32320"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX8112476.1"
                     /translation="MSESTDASSRSEIRLVTHLFDRRAESTPDAPCIRHGAVSWTYAD
                     LKARVDTLADELVARGAGPETVIAVFLPRGADLATAVLGTLQAGAAYLPIDVAAPAAR
                     IAALLRAANARFVLTGPERAERLPAGDHTVLDITQVTARHGSARTAPGPAGLDSLAYV
                     IFTSGSTGEPKPVAVTHRSLANHAQAIQERYRLHAGDRVLQFANPAFDVFAEELYPTL
                     CAGGEVVVVPDPTMPPMELCRFLDERTVTVVNLPTSYWSQWTRDLETGAAVVPGHLRL
                     LVVGSEPAYRETLVRWRRHSALPVINAYGLTETTVTVTTEMFEAAPDDTDTLPIGRPL
                     AGCTALVLDSDLVPVPPGTAGELYLGGACLARGYLGMPARTADRFVPHPHPPEPGARL
                     YRTGDLVRQRPDGALEFLGRLDGQVKVRGHRIEPAEVAAALTSHPLLAQAYVTTDRDA
                     QGVVRLVAYVVPADTRRVPTGAMLRTHLSEQLPAYMVPSAFVVLDALPLTPTGKVDVR
                     ALPPVRALQSAAPYVAPEGALEERLAEIWREVLGVPRVGREDDLFDLGAHSLTAARIA
                     ARIRAELGHELGVPDLFEHPTIAALAAWLAGDHGSRPALPAITPHPGEDSAPLSYQQL
                     QMWFLTKLAPNSIAYHAQTTIRVVGALDLGVLDRTVTEIGRRHEILRTTYHEIDGEPR
                     QVVQPAGPVAVRRVDLTGVPAAERAQRVEELVQEELHLQFDLTRLPLLRWTAIRLAPD
                     EYELVLVEHHLVHDGWSFALLMRELREIYGAFAVGRGSPLPELSVQYRDYARWQREAM
                     DSQVMRAQLAYWREQLAQLPPALALPQDWPRPAVQSYRGETLRIELPPTLPSALRTFC
                     RAERITLYTAMYAAFTVLLRRYSGERDVCVGSAYANRQSHQTQDVIGMLVNVTLLRLA
                     VDDRRSFRELARQARDVVLGASKNQELPFPALVRELNPARDVTGTPLVRILFSANDSP
                     MPELDLGGATGTVYERGNGSAKMDLDVVVIPRAESQMGDAGHTDDRILLMWEYNADLY
                     DPQTMRRMVEAYLRLLEDCVRRPDTALADLRLLAEPEQERILRAYNPSPAGEAGPPVH
                     LAVAAHARAHPDAPAVITSSERLSYAELDRRAGWLAMQLRELGAGPERIVGICLPRGT
                     DLIVAQLGILRAGAAYLPLDPDYPADRLAYLCADAGARLVVSAGPYAAKVPATRRRVV
                     LDNLPGGPPPAEPPSRVGPHTMAYVIYTSGSSGKPKGVVVDHGALSNLVRWHRAEFQL
                     TSADRISTVASPSFDASVGEIWPALAVGAALHVPDPEVRLAPSRLQAWLLEQRITVSD
                     LPTPLVEALLVLPWPASTALRTMISGGDRLRIRPSAGLPFQLVNTYGPTESTCMVTCG
                     PVAPAAGRMGAPDIGRPIPGTTAYVLDTELRPVPVGVPGELYLGGAGLARGYLGRSDL
                     TAQRFVPDPFASGGRLYRTGDLVRHSADGALEFLGRTDAQIKIRGFRIEPGEIVAALL
                     AHPAVADAHVTARTLAGSADPQLVGYLVAAPGAAQPSPAQLREHLVATLPVHMVPGRY
                     AWLPALPLTRNGKIDERALPEPPAPPIAVSAAPDTALERRLVEIWRQVLGIEQVGVHD
                     SFFDLGGHSLQLGQVHHLLVGELKRDLPMLDLFQHPTIHALARHLEGGARAPEPDRGA
                     ELAAQRIDARSRLSRRRGNLPGQTQ"
     gene            14758..15321
                     /locus_tag="VF516_32325"
     CDS             14758..15321
                     /locus_tag="VF516_32325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112477.1"
                     /translation="MLRSSVVIAVVLVLGTVTIAHADELASAAAPAPALAPAPPAAPA
                     PEASPLNYVGLGTATTLFHKPNSGPASGWQQDIAPVIGYGRYVTPTIALELDFGPEFV
                     RGSGYSGFFLVPAAVWSFSTHFYAAARFLVEADPQIDFALFPGIGAIYTFPNQVSVGL
                     ELNVESFIGRGNPGLAIALTPGIEYSF"
     gene            15476..>15796
                     /locus_tag="VF516_32330"
     CDS             15476..>15796
                     /locus_tag="VF516_32330"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8112478.1"
                     /translation="MLLDDFVQSAEAHPDRALFVHAGGALSYGAAAAAIARAIEPLRA
                     LAASPGSRVLCLAEDTIPLALLLLAAGYAGVIPAAVSPLFSTEYVAAVARQIDARAVF
                     TTPAF"
BASE COUNT         2343 a         5536 c         5628 g         2289 t
ORIGIN      
        1 cgagcggtac ctcgaggagt ccaagcttct cttcgctccg aagctgcgcg aggcctgcgc
       61 ggcgacgaag acgcgggggt ccgactacta tcggggaaag gtccggggtt agcgcatcga
      121 gcatggcgcg gcttcgccgc gcggagctca tcgcaggagg cgcggaacgt tttgagggtc
      181 gccgcggtct cgccgctcgc tcgccagcca gcgacccgct gccgccacac cgttgctgtg
      241 ttcaaggtcg gcggcgtgcc ggcgttcgcg ggtggcgcag gcatcttgct cggcacgggc
      301 ttcggcgagt actatgccgt gcgcggctga tgtcgtccgg acctcccggc gcgctcaatc
      361 ttgcgccatc agcgtcttca ggaacgcgta gtggaagtcg accccccggt agaaggactc
      421 caccggggtg cgctcgtcct tgccatgcgc gcgaagatcg tcgcgatcca tggcttcgcc
      481 attgacctgg tacacgggga tgccggcagc gctcacgtgg acggcgtcgg acgcacccac
      541 cgcgagcatc gggatcaccg gcacgcccgg ccacatcgag gcggcgacct gctccagcga
      601 tcgcatcacg tcctggcgga gcggcgccgg ggtgaagccg tgcttgctct cggcggtatc
      661 gagcacccgt ccgtcatccg ccacgtagcg cacggtgatc cgcggatccg cgagcacgcc
      721 gatcagcgcc ttgcgcacct gttcggcaga gtgtcccggc aagatgcgac agttgacgtt
      781 tgcgcgcgcg gtctggggca gcgcgttgtt cgcgtggccc gcctcgagcg cggtggcgac
      841 acaggtggtg tgcgtgacgg cgtgatccag cggatcgccc gccaggcgcg cgatcgcggc
      901 cggatcgggg ggcaccttga gaatggcgcg catgtccgcc gcacggctgc cggtctccag
      961 ggtcgcctgg cgttcgtagt acccgcgcgt cacgttgctg agctcgaagg ggaactcgta
     1021 tcgcgagagc cggtcgagcc cgccggtgag gtgatagatg gcattgtcgg gtaccggcat
     1081 cgagctgtgg cccccggggc tcgtgaccgt gagctggaag tcggcgtaca ccttctcgga
     1141 ggcgtcgatc tcgacgtaca gggccttgcc gtgctccatg accacgtcgg gaccgtcgtt
     1201 gctgagcgcg aactccgcat cgaccagctc gcggtgatgc ttgaacagcc aggcgacccc
     1261 attcgagctg ccgctctcct cgtccgccgt gagcgcaagg atgatgtcgc gatcgggccg
     1321 atagccttcc ttcttgaagc ggatgagcgt ggtcagcagg atggcgtcgc catccttcat
     1381 gtcctgcgtg ccgcggccgt agtagtagcc gtcccgctcg acgagctgaa acggattggt
     1441 ggtccagtcc tcgcgccgcg cctcgacgac gtcgaggtgg ccgatcaaga gcagcggctt
     1501 gcgcttgccc gtaccacgca agcgcacgac gaggttcttc ttgcgctcgt cgggccccac
     1561 gacgtggatg tcgctctcgg ggaagccggc ggcgcgcagg cgttgcgcca tcgcctcgga
     1621 tgcggcggtc acgctcccgg tggaaccggt cgtgtcgatc tcgatcagct gctggaacag
     1681 gtcatgcgcg agcttccggg tcgccgcgtc gacgtcggcg cgtgcagcgc ccgggcacag
     1741 gagggcggcg attccgagca tggcggctac ctggttcatg caggatctcc ggtcaggatg
     1801 aggtcctccg tcaggaggcc gtgggcagcg cgggacagcc accgctgact gacgcgggcg
     1861 tggtcaggct ccgggccggt cgatagccgg cgccaggcgc cctcgacccg atgccgggcc
     1921 gcaccgcagt agacgtctgc gaggtccctc gcctcgacct ggccctgctg cgccaggtgc
     1981 gcggcccgcg cgaggacgac cgccatcgtg aacagctccc gggtgagctg gcccagcccc
     2041 atcacgatgg attcgcgggc gtacagcgcc tccggatccg ggtaccgctg gacgagctcc
     2101 acacaggtcc gcgccagccg ggtgacctgg ctgccggtgt actccaggtg gtccgcgttg
     2161 cgcgcgtcca gcccgggcat cgctggtctg gtttcggccg gcggcaatgc accgggccgg
     2221 gtgctccccg agcccggtcg atccgagggg tagtagcaag cgagcaggcc ggattgagcg
     2281 gcccagaagt cgacctggaa gtccacccca ccggcgatgc gcagacctcg ggcgtcgcgc
     2341 aggaaccgct cgacgggcag tccgggcgct cctcggcgcg ccttgctcgg ccccgtctcg
     2401 tagccctcgg cgcccagcag cgacatggtc cggtcgatga tccgccagct ggtcagcgag
     2461 gtgatgttct tggccgccac ccgttcgtac cgggtgtccg cgcgacgggc cccgcacagc
     2521 gcccagatca ccacgctatc gatggcgaag gcctcggcca gggacagcgc caccatgcgc
     2581 tggatctggt cgtactcgcc gagcgggcga ccatccacgt tcctgcggca caaaaagtcg
     2641 cgagaccagt gcgcgcagag cttggcgatc gccagcgacg gagcgccgat gatgtagatc
     2701 cgtgcggtgg cggccatcaa ggagatctgt ggcgacagcc gccattcgtc ctcgtcgccc
     2761 cccagcatgt gctcgcgcgg caccctcacg tcgcgcagca ccagccgggc gttggacgcg
     2821 ccgcgcaggc cgaggaactc gtgccgcccg gcgacctgga agccggggct gcgcgtgtcg
     2881 acgaagaaca gccgtacctg ctcgcgaccg ccctcccgca gggtcgccga gacgtcgagg
     2941 aggtcggcct ccggcccgtt cccgatgaag atcttctcgc cgttgagcag gtacgccgcg
     3001 ccgttctcga tcggtgtggc cgtcgtcgta cgcagccggt tggctgcccc cgcgggctcg
     3061 gtgtccgctc cgccggacac gatgccctgg gccacccggg cggtgatcag atcgcgcagc
     3121 ggccccgccg gcaagaacgg gaggtacgcg gcggagccga gcccgttgtg gatggccatg
     3181 gccagcgcca ccggggggga ccagctcgcg gtggcctgga tggcccggaa ggtgttgtac
     3241 ggcgacagcg ccaggccgcc gagcggccgg tccaccgtca gccgcaaata gccgcgctcc
     3301 tgcagctggc cgaacaggcc gtcggggagc cggcgggtct cgtcgacctc ggtcggatcg
     3361 acgcgccgcc ggaggaactc cgacagctcg gcgacgaccg cgtcgccctg cctccgctcg
     3421 tccggatcct gctccgggaa tggctggagc agatcgcagc ggagccggcc gagaaacagc
     3481 tcggccaaaa accctggctg cccgctcacc ccgccgtcct ccgcggagcc gccaccgagg
     3541 aacgagacct gctcgccagg gtggaggaga ggaagcgcag aaggccgtcg cgctgcttcc
     3601 gcaggtagaa atggtcgccg gtgaaccgct gcagggcgaa cgggccggtc gtgtgccgcc
     3661 gccacgccct cagcatgtcc tcggggtagt cctccgtgcc gccgaccacg gtcagcggca
     3721 ccgacagcgg cggcccctcg gcgtggcgat aggtctcgat catctcgaag tccgcccggt
     3781 ggtacgccag gatgagctgg cggaagtccg ggtcgtcgtg cagctcgggg gacagctcgc
     3841 cgaactgccg ctccacgatc ggccacagtt gctcgtcggg cagccggtgc acctcggggc
     3901 tggtgctggg cagatgcggc gcgtggtagg cggagacgat cagggcctcc ggcttcggtc
     3961 caccccggtc acggagggcg cgcgcggtct cgaacgcgac caccgccccg aggctgtgcc
     4021 cgaacagcgc gaacggaggc tggaacgcga ccgcgtcgac gatcgcctcg acgagagcat
     4081 ccacgcgcag gtagcactcc tcggccaccc gcgacccgcg gccgggcagc tgcactcccc
     4141 acacctcgat tccagggagc ccgtccgacc agcgcacgta ctctcccgcg gacccgccgg
     4201 agtgaggaaa gcagtacagc cgcacggcgg cgtcaggacg gcgctgccgg catgccagcc
     4261 aggccgaacc ggcatccggg tgagatgccg caggccgggg atgagacatt cctggatcgc
     4321 gatcagccct gggcggtcat cacttcttcg gccaccgcgc cacccggggc cggttcggcc
     4381 accgcgtcac ccggggacgg ttcggccacc gcgtcccccg gggccttggg gagctcgcgc
     4441 aaggtacgaa ccggagacag catgagcggc aggctggcca gcgcgctgcc gatgcccgcg
     4501 atcaccagcg tggggcgcag gccgatggtg ccgccgagca ggccgcccag gaaggtgccg
     4561 atcggcaggg tgccccagat gaggacgcgg atggttgcgt tcatccgccc ctgcatcgcg
     4621 tccggcgtca cggtctgccg cagggtgatc tggttgatgt tgtagatgga gatggcggcc
     4681 ccggccagtc cgagcgcgat cgagagcacc ggaatcgcgg tctccggggt ggccagcggc
     4741 aacagcagga cggcgagccc ggggatgacg gacgagaccg cgatcatggg acctaccccg
     4801 aagcggcgaa tcagccggcc gttgagggtg gcgccgagca gagcgcccgc gttggccacc
     4861 gccagcaccc cgcccagggc caccggggtc aagtgcagct cgcgcaccgc gtagagggtg
     4921 aggatggcgg cgaccatccc gaacagatcg aagagattgc caatgccggt ggtgagcgcg
     4981 atcggccgga gcagcgagtg gcccagcacg tagcgcagcc cctcccggat ctgcttgccg
     5041 atgccggcct cctccccctc ggggcgggcg ggcgccggct ccggggtgcg aatcgcgaac
     5101 aagaggacgg ccgacaggag atagctgagc gcgtcggcga cgatggcgat cggcgcggtc
     5161 aggaactgga cgagcgcccc gccgaggccc gggccagcca gctgggcgcc cgagtaggag
     5221 aactccagct tggcgttgcc ctcggtgagc tgttcgcgcg gcaccagcga cggcaggtac
     5281 gactggtggg cgacgtcgaa gaacagggtc aggatcccgg cgctgaacgc gatcgggtac
     5341 agcatccaca ttcggagccc gtccagcgcg tacgcgatcg ggatgatgag caggatggcg
     5401 acccggccca ggtcggccac gatcaggatc ggccggcggc gcatccggtc gacccagact
     5461 ccggccggca gccccaccag gaggaccggc aggtacccgg cggccgtgag caggccgatc
     5521 tgcatcgtgg aggcgttcag cagcaggatc gccgtcagcg ggatcgcgag caggctcacc
     5581 tgcgagccga gcaggctgat gctctgggcg ctccacagca tcatgaagcc acggtgccgc
     5641 cagaggttcg acgggcgctt gagtggggtc acggcgctca tcgcgactcc atccgttccg
     5701 cggtccggag gctgcgcggg cgcagatcct tccaggtgcg ctcgatgtgg ctcagacact
     5761 cggccttggt gcccgcgact ccctgcgcgg tccagccggc cggcggcgcg cggtcggcgg
     5821 gccagatcga gtattgctct tcctcgttgc ggaccacgca gtacggacga ttgtcgtcat
     5881 cttcgaacat gggttcaggc ttcctctctg gaaagcgatg gggtgggggt ggaaaggcgg
     5941 cggtcgagca gcgcggccag cgcctggggg gtcggtgcgg tgaatacttc cttgagcgac
     6001 aagctcgtct gatagctgcg tcggatccag gcggtgagct gggtcgcgat cagcgagtca
     6061 cctcccagct ggaagaagtt gtcgccgggg tggacccggg cgagaccgag gatctcggcg
     6121 aagatcccgc agaccgcctg ctcgcgctcg ctgcccgaag caggcggtaa tgcgggcgac
     6181 gcgtcgtcct ctcggggcag gtccggcagc aagatcggct ccgcgccggc ccgatcgacc
     6241 tgcgcttccg ccggagcctc cacgaggtag cgctggccct ggaacgggta cgtcggcagg
     6301 gccacgcggc ggcgccgctc gccctcgtgc aaccgggtcc agtcgagcgg gactccggcc
     6361 gaccacagcc gcccggccgc cgccagcgag accgcgagat ccgaggtgga ctcggcggcg
     6421 tgcgacagca tgggcaccgc cagcgtgtcc ccgaagcgcg gatgctggcg agccagcgtg
     6481 atcaaggtcc ggccgggccc gacctcgacg agcacctgcc ctggcgtgcc gagcaccgtg
     6541 gccagcgcat cggcaaatcg caccggctgg cgcaggtgcg cggtccagta gtccggtcgg
     6601 gtgggcccgt cggcggtggc ccaggtgccc gtggtgtccg agacccaggg caccgacggg
     6661 gcgcgcagat ccagctggga cacgcgcgcc tcgaactcgg cgaggatcgg atcggccagg
     6721 gtcgagtggg caccggcggg gatgtgcaac cgccgcgcat cgatcccgcg tgcggtcagc
     6781 gctgcggcga gcgcgccgac ccgagcggcc ggcccggaca ccacgcactg gttcaccccg
     6841 ttgatcgccg ccagggagag ctcctccccg agcagctcgc ggacttcctg ttccggcaag
     6901 cgaacagcca gcatggcgcc ctcggccagg cgatccagca gccggccgcg ctccaccacc
     6961 aggcgcaggg catcggggag ggagaacacc ccggcggtgc acgccgccgc gtaggcaccg
     7021 aggctgtgcc ccagcaccag gcccggctgc actccccacg ccgccagcag ctgggccagc
     7081 gcgtattcca cgacgaagac ggtcacctga cccagcgcca tcgtggccag ctgctgccgg
     7141 gcgcgctccg gatctccggt gggatacagc accgtccgca ggtcgagccc gagcggcgcg
     7201 ctggccagtt cggcgcactc gtcgatcgcg gccgcgaacg ccggctggtg ctggtacagg
     7261 tcacggccca tcccgacgtg ctggccgccc tgtccgggga acaggaacac caccgagggg
     7321 cgccccgctt cggccacggc acccatgccg gcggagctcg cctccgccag cgccgccgcc
     7381 gcgtccgcgg ctcgctcggc gaccacgatc cgccggtagc gctgctcctg ccggcccacc
     7441 tgcaacgtcc acgcggcatc ggccagcgcg aggtcgggat tccggcgcag atgctccgcc
     7501 aatctcccgg tggcggcatc gagggcgctg cgcgtcctgg cggagagcac cagcaggtgg
     7561 tgcggggggc ctggatcggc cggaggctcc ggccactcct cgagcacgac gtgcgcgttg
     7621 gtgcccccga tgcccagcga gctgaccccg gcccgacgcg gcgtcccggc ggcgtcccac
     7681 ggcgtccggc aggtcgggac gacgaaggga cttccggcga ggtcgatctc cgggttgggt
     7741 gcgcggaagt gcaggctcgg cggcagctcc cggtgctgca gcgcgagcac cgtcttgatg
     7801 aatcccgcga cgcccgccgc cgcgtcggta tggccgatgt tggtcttgat ggagccgatc
     7861 cggcagaatc cccgccggtc ggtgccctgc cggaacgcct tggtcagcgc tgcgatctcg
     7921 atcgggtcgc ccagcggggt ccctgtcccg tgggcctcga tataggtgat cgtctccggc
     7981 gcgacctcgg cgatcagctg agccgcccgg atcacccgcg cctgcccctc gatgctcggt
     8041 gcggtgtacc cgatcttgtc gaagccatcg ttgttgacgg ccgacccgcg gacgacggcg
     8101 cggatcggat ctccgtcggc cagcgcgtcg gcgagccggc gcaacaccac cacgccggcc
     8161 ccgtcgccgc cgaccgtgcc attcgccgcc gcgtcgaacg cccggcagtg gccgtcccgc
     8221 gacaggatcc cgccctccat gtactccccg gggtccgccg gcacgtggag ggtcacgccg
     8281 ccggccaggg ccatgtcgca gtcgcccgcg agcagcgcct gggcggccat gtggatggcc
     8341 accagcgagg tggagcaccc ggtctgcacg gtgatcgccg gcccggtcag ccccagcttg
     8401 tacgccaccc gggtggtgag gaagtccatc ccggtggcga tccggatctg gaactcggac
     8461 accgctccga gccggcgccg ctgcgacatc agcacgctgg ggtactgggt atgggtgctc
     8521 ccggcgtaca cgccgatcgc acccgggtac cgggcggggt caactccggc gtcctccagc
     8581 gcggcccacg agcactcgag gaacacccgg tgctgcgggt cgagcaggag cgcctcgcgc
     8641 ggcgcgtagc cgaagaagcc cgcgtcgaac cggtcggcgt tctcgaccac gccgcacacg
     8701 gcctgatgcc cggtgacggg atccagagga cccagcggcg agatcgactc cacgccgccg
     8761 acgaggttgt tccacagctc gtcgggggtc tccgcgccgg gaaaccggca ggccattccc
     8821 accaccgcca gccacgggct atcggcaggc ggtcgttcag ccccggcgct cactgcgtct
     8881 gccccggcag attgccgcgt cggcgggaca gccggctccg cgcgtcgatg cgttgcgcgg
     8941 ccagctcggc tccccgatcc ggttctggag cgcgggcgcc cccttccaga tgccgggcga
     9001 gggcgtggat cgtggggtgc tggaacaggt cgagcatcgg caggtcccgc ttcagctcgc
     9061 ccaccagcag gtggtggacc tgaccgagct ggagcgagtg accgcccaga tcgaagaagc
     9121 tgtcgtgcac gccgacctgc tcgatcccca gcacctggcg ccagatctcg accagccggc
     9181 gttccagtgc ggtgtccggc gccgcgctca cggcgatcgg aggcgccggg ggctccggca
     9241 acgcccgctc gtcgatcttg ccgttgcgcg tcagcggcag cgccggcagc caggcgtacc
     9301 ggccgggcac catgtgaacc gggagcgtcg ccaccaggtg ctcccgcagc tgcgccgggg
     9361 aaggctgcgc cgcgcccggc gccgccacga ggtacccgac cagctgtgga tctgcggacc
     9421 cggcgagggt gcgcgccgtg acgtgcgcgt cggccaccgc cgggtgggcg agcagcgcgg
     9481 cgacgatctc gcccggctcg atgcggaacc cgcggatctt gatctgggcg tcggtgcggc
     9541 cgaggaactc gagggcgccg tcggcgctgt gccggaccag atcgccggtg cggtagagcc
     9601 ggccaccgga ggcgaacggg tcggggacga accgctgcgc ggtgagatcg gaccgcccca
     9661 ggtacccgcg ggccagcccg gcgccgccca ggtacagctc gccgggcacc cccaccggca
     9721 ccggccgcag ctccgtgtcc agcacgtatg ccgtggtgcc ggggatgggc cggccgatat
     9781 ccggagcacc catccggccc gccgccggcg ccaccggacc gcaggtgacc atgcacgtgc
     9841 tctcggtggg cccgtaggtg ttgacgagct ggaacgggag gcccgccgag ggccggatcc
     9901 gcagccggtc cccgccgctg atcatggtcc gcagggcggt gctggccggc cacggcagca
     9961 ccaggagcgc ctcgacgagc ggcgtcggca agtcgctgac ggtgatccgc tgttcgagca
    10021 gccatgcctg caggcggctc ggcgccagcc ggacctcggg atcggggacg tgcagcgccg
    10081 ctccgacggc gagcgcgggc cagatctcgc ccaccgaagc gtcgaagctc ggcgaggcca
    10141 ccgtgctgat ccggtcggcg gaggtgagct ggaactcggc ccggtgccag cgaacgaggt
    10201 tggacagcgc tccgtggtcg accaccaccc ccttgggctt tccgctcgag cccgaggtgt
    10261 agatgacgta ggccatggtg tgtgggccca cgcgggacgg cggttcggcc ggcggcggac
    10321 cgccgggaag gttgtcgagg acgacgcgac gacgggtcgc cggcaccttc gcggcgtacg
    10381 ggccggcgct cacgaccagc ctggcccccg catcggcaca caggtacgcc agccggtcgg
    10441 ccgggtagtc cgggtccagg gggaggtacg ccgccccggc gcgcaggatg ccgagctggg
    10501 cgacgatcag gtcggtgccc cgcggaagac agatcccgac gatgcgctcg ggtccggcgc
    10561 cgagctcgcg cagctgcatg gccagccagc cggcgcgccg gtccagctcc gcatagctga
    10621 gccgctcgga cgaggtgatc accgcggggg cgtctggatg ggcccgcgcg tgcgcggcga
    10681 ccgccaggtg cacgggcggc ccggcctccc cggcgggcga cgggttgtac gcgcgcagga
    10741 tccgctcctg ctcgggctcg gcgagcagcc gcagatccgc gagcgcggtg tccggacggc
    10801 ggacgcaatc ctccagcagc cggaggtacg cctcgaccat ccggcgcatg gtctgcggat
    10861 cgtagagatc cgcgttgtac tcccacatca gcaggatgcg atcgtcggtg tgtccggcgt
    10921 cgcccatctg gctctcggcg cgcgggatca ccacgacatc gagatccatc ttggcggagc
    10981 cgttgccgcg ctcgtacacc gtcccggttg cgccaccgag gtccagctcc ggcatcggcg
    11041 agtcgttggc gctgaagagg atccgcacca ggggggtgcc ggtcacgtcg cgcgcggggt
    11101 tgagctcgcg gaccagggcc gggaacggca gctcctggtt cttcgaggcg cccaggacca
    11161 cgtcgcgggc ctgccgggcc agctcccgga agctccgccg atcgtccacc gcgagccgca
    11221 gcaacgtgac gttcaccagc atgccgatga cgtcctgcgt ctggtgggac tggcggttgg
    11281 cgtacgccga gccgacgcac acgtcgcgct cgccggagta gcgccgcaag aggaccgtga
    11341 aggcggcgta catcgcggtg tagagcgtga ttcgctcggc ccggcagaac gtccgcagcg
    11401 cggacggcag ggtcggcggc agctcgatcc gcagcgtctc gccccggtag ctctgcacgg
    11461 ccggccgcgg ccagtcctgc ggcaacgcca gcgcgggcgg cagctgggcc agctgctcgc
    11521 gccagtacgc cagctgggcg cgcatgacct gggagtccat ggcctcgcgc tgccagcggg
    11581 cgtaatcgcg atactggacg gagagctcgg gcagcggcga gcctcggccc accgcgaagg
    11641 ccccgtagat ctcccgcagc tcccgcatca gcagtgcgaa cgaccagcca tcgtggacca
    11701 ggtgatgctc gacgaggacc agctcgtatt cgtccggcgc aagccggatc gccgtccagc
    11761 gcagcagcgg cagtcgcgtc aggtcgaact gcaggtgcag ctcctcctgg accagctcct
    11821 cgacccgctg tgcccgctcc gccgcgggca ccccggtcag gtcgacccgg cggaccgcga
    11881 ccgggccggc cggctggacg acctgcctgg gttcgccgtc gatctcgtga taggtggttc
    11941 gcaggatctc gtgccggcgc ccgatctcgg tcacggtgcg atcgagcacg ccgagatcca
    12001 gggctccgac cacgcggatc gtcgtctgcg cgtggtaggc gatgctgttc ggggccagct
    12061 tggtcagaaa ccacatctgg agctgctgat aggacagcgg tgcgctgtcc tcgcccgggt
    12121 gcggggtgat ggcagggagc gccggccggg acccgtggtc gcccgcgagc cacgccgcca
    12181 gcgccgcgat cgtggggtgc tcgaacaggt ctggcacccc cagctcgtgg ccgagctcgg
    12241 cgcggatccg ggcggcgatg cgcgccgccg tcagcgagtg ggcgcccagg tcgaacaggt
    12301 cgtcttcgcg gcccacccgc ggcaccccca gcacctcccg ccagatctct gccagtcgct
    12361 cctccagcgc gccttccggg gccacatacg gggccgccga ctgcagcgct cgcaccggcg
    12421 gtagcgcgcg cacgtcgacc ttgccggtgg gggtgagcgg caacgcgtcc aggaccacga
    12481 acgccgaggg caccatgtag gcgggcaact gctcgctcag atgggtgcgc agcatggcgc
    12541 cggtcggcac tcgccgggtg tcggcaggca ccacgtaggc gaccagccgc acgacgccct
    12601 gggcgtcgcg atcggtggtg acataggcct gcgccagcaa cgggtggctg gtcagtgcgg
    12661 ccgcgacctc tgccggttcg atccggtgac cgcgcacctt gacctggcca tcgagccgtc
    12721 cgaggaactc cagggccccg tcgggccgct gccggaccag atcgccggtg cggtagagcc
    12781 gggcaccggg ctcgggcgga tggggatggg ggacgaaccg atccgcggtg cgcgcaggca
    12841 tccccaggta cccccgggcc aggcaggccc cgccgaggta cagctccccg gccgtaccgg
    12901 gcggaacggg caccaggtca gaatccagga cgagcgcggt gcagccggcc agcggacggc
    12961 cgatcggcaa cgtgtcggtg tcgtccgggg ccgcctcgaa catctcggtg gtgaccgtca
    13021 ccgtggtctc ggtcagcccg tatgcgttga tcacggggag ggccgagtgc cggcgccagc
    13081 gcaccagggt ctcccggtag gccggctcac tgccgacgac gagcagccgc aggtggccgg
    13141 gcacgacggc cgccccggtt tccagatcgc gcgtccactg gctccagtac gaggtcggca
    13201 ggttgaccac cgtcaccgtc cgctcgtcga ggaagcggca cagctccatc gggggcatcg
    13261 tcgggtcggg gacgaccacc acctcgccac cggcgcacag cgtggggtac agctcctcgg
    13321 cgaacacgtc gaacgccgga ttggcgaact gcagcactcg atcgccggcg tgcagccggt
    13381 atcgctcctg gatcgcctgc gcgtgattgg ccagcgaccg atgcgtgact gccaccggct
    13441 tgggctcgcc cgtcgagcca gaggtgaaga tgacatacgc caggctgtcc agaccggccg
    13501 gccccggtgc ggtccgcgcg gagccgtggc gcgcggtgac ctgggtgatg tccagcaccg
    13561 tgtgatctcc ggccggcaac cgctcggcac gctcgggccc ggtcagcacg aagcgggcgt
    13621 tggcggcgcg gagcagcgcc gcgatcctcg cagcaggcgc ggcaacgtcg atcggcaggt
    13681 aggccgcgcc cgcctggagc gtgcccagaa ccgctgtcgc cagatcggct ccccgcggca
    13741 agaacaccgc gatcacggtc tccggccctg ccccgcgtgc caccagctca tccgccagcg
    13801 tgtccacgcg agctttgaga tctgcgtagg tccacgacac ggctccatgc cggatgcacg
    13861 gcgcatccgg agtggactcc gcacgccgat cgaacagatg tgtcaccagg cggatttcac
    13921 ttcgggacga tgcatcggtc gattcagaca tggcgctccc cttcggtagg ctttgcggct
    13981 gtcgcctggc agccgctcaa tctgtgggtg ccgcctggct cgaggctgcg gcgggcagct
    14041 gtgggccctg tgatagcggc cctggcgctg caactggaat gcggatgtct aacacgcaaa
    14101 tgcatgatgg tgtccacaca gcttggacgc attcgacatg cgaccggagg cgaagggcaa
    14161 agcgaagggg ccatttgcta ccacactgca ctgcctcact ccaaccctat tttgtcgaag
    14221 tgccggtgtt aaacagatcc tgcaattcgg atcacaagca cttttgaatg acaaaaagtg
    14281 ttcccagcgc gagagagtga gagagtgaga gaagttagct tctaacagag gataagtttc
    14341 gtaatactta acaccgtttt tccgttcgac tttcgaaagc agttttcaac tttcgtgcca
    14401 cgggtcgcca ctcgacggat cgcgcaggta gctaaattaa ctggttcttc tcgcgcttcg
    14461 gtgatggagg tcacgtggga ttcgtgcgca cgcacgagcc tccgaagatc ccgaggtctc
    14521 aaagccagcg acgagaacgc atcacctgtt aacgattgaa tgagtcgtca catggggtgg
    14581 ggtcagtcac attcgcgcgc ttctgtcatc ggtgtgacgc acacgctcgc tcgatgacgc
    14641 accgaatttc acgagttgat gtcctcattt cggactcgtg ttgccgatcg cacggtttgt
    14701 cctggcgcgg tctcgacaca ttcgaccacg agtaggttag cgtctcgggc catgtgtatg
    14761 ctccggtcct cggttgtcat cgccgtcgtc ctggtcctcg ggaccgtcac catcgcccac
    14821 gccgacgagc tcgcatctgc agcggcgccg gcacccgcgc tcgcgcccgc gcctccagcc
    14881 gcgcctgcgc ccgaagcttc gccgctgaac tacgtggggc tggggaccgc cacgactctg
    14941 ttccacaaac cgaactcggg acccgcgagc ggctggcaac aggacatcgc tccggtcatc
    15001 gggtatggcc ggtacgtcac ccccaccatc gccctggagc tggacttcgg ccccgagttc
    15061 gtccgcggca gtggctattc gggtttcttc ctcgtgcccg ccgcggtctg gagcttctcg
    15121 acgcacttct acgcggcggc tcggttcctg gtcgaagcgg atccccagat cgacttcgct
    15181 ctgttccccg gcattggcgc gatctacacc ttcccgaacc aggtctccgt cggcctggag
    15241 ctgaacgtcg agagcttcat cggccgaggc aaccccggtc tggccatcgc gctgacgccg
    15301 ggcatcgagt actcgttctg atctggaacc ctcgcgccgc agggacccgc gcttcgtcgt
    15361 ggagatccag ctcccgcggc ggttccgctc ggatgcgcag gatcgccggc atgggcccca
    15421 gcgccgccgg ctcgctgcgg tatactgcgg cgcacgaccc acggggtcgg tgccgatgtt
    15481 gctcgacgat tttgtacagt cggcggaagc acacccggat cgcgcgctgt tcgtccacgc
    15541 tggcggcgcg ctgagctacg gggcggcggc cgcggcgatc gcgcgtgcga tcgagccgct
    15601 gcgtgcgctc gccgcctcgc cgggcagccg ggtgctgtgc ctggccgagg acaccatccc
    15661 gctcgcgctg ctcctgctgg cggccggcta cgccggcgtg atccccgccg cggtgtcgcc
    15721 gctgttctcc accgagtacg tggccgcggt cgcgcggcag atcgacgcgc gcgccgtgtt
    15781 caccacgccc gcgttc
//
LOCUS       DASVOR010001736         2634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_193509,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001736 DASVOR010000000
VERSION     DASVOR010001736.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193509"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(189..1820)
                     /gene="pruA"
                     /locus_tag="VF516_32335"
     CDS             complement(189..1820)
                     /gene="pruA"
                     /locus_tag="VF516_32335"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEX8112479.1"
                     /translation="MIDATVTVPPAVNEPILGYAAGSPERTRLLAALAAIEREVVEIP
                     CVIGGQRVHTGKLRDVVMPHRHKHVVARFHAATEDVAERAIRAALDARREWAAMRWEA
                     RAAVLLRAAELLSTTWRMRLNAATMHGQSKTAHQAEIDSACELIDFWRFNVQFAAELY
                     RVQPVSTHGVWNMTDLRPLEGFVFALTPFNFTAISGNLPAAPALMGNTVVWKPAETQL
                     LAAWQVYQLLEEAGMPPGVINFIPGLPHGSTNAVLDHPMLAGIHFTGSTDVFRMMWKR
                     VAENIDRYRSYPRLVGETGGKDFILAHPSADPQALATAIVRGGYEFQGQKCSAASRVY
                     VAKSVWDRMREPLLDTIRSLKIGDVSNFSNFMGAVIKQTAFDKHQAAISEARETSGMK
                     VIAGGICDGREGWFVHPTLITTEDPSVRHMREEFFGPIVTLHVYPDAAWHDTLQLVDR
                     TSPYALTGSIFARDRAAIVEATTALRDAAGNFYINDKPTGAVVGQQPFGGARASGTDD
                     KAGSALNLPRFVAARTIKETFAPPTDWRYPFLDQP"
     gene            1925..>2634
                     /locus_tag="VF516_32340"
     CDS             1925..>2634
                     /locus_tag="VF516_32340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112480.1"
                     /translation="MRWTLACLLAAGCGRLGFDSRVQPAGDDTVMPDGAANLVFVTSQ
                     HVVVGQLGTLAAADAVCKQAAAGAGLPGNYVAWLSTSKTSAIDRLAGARGWVRPDGRP
                     FADTPADIAAGRIWYPASIDENGAVAATLVVTTSDAHGRFDGMSCNDLTDDSIDGSCD
                     FGESTGTAGTFSSYPLLGGLCFVDEPMYCFATDRNVRVQAPRPTTGVRVFPAEGFTPG
                     GGLASADALCARTAAAAGL"
BASE COUNT          360 a          913 c          977 g          384 t
ORIGIN      
        1 cgagaacatc gcggacggga tcgcagggcg cacgccgcga gcgtatcacc gcgccggcgc
       61 cggcgaccgc gccgtcgccg gctgccagca cgcgctggtc gatgtcagcg gcgatcgtgt
      121 gacgtgcgcc gcgttgcgac ccgccggccc cgagtctctt gcgcggcgga tcacggtcgc
      181 ctcggcgatc acggctgatc gaggaacgga tagcgccagt cggtcggcgg cgcgaacgtc
      241 tccttgatcg tgcgggcggc gacgaaccgc ggcaggttga gcgccgagcc ggccttgtca
      301 tcggtgcccg acgcccgcgc gccgccgaac ggctgctggc cgaccaccgc gccggtcggc
      361 ttgtcgttga tgtagaagtt gccggccgcg tcgcgcaggg cggtcgtcgc ctcgacgatc
      421 gccgcgcgat cgcgcgcgaa gatcgagccg gtgagcgcgt agggcgacgt gcggtcgacc
      481 agctgcagcg tgtcgtgcca cgcggcgtcg gggtagacgt gcagcgtgac gatcgggccg
      541 aagaactcct cgcgcatgtg gcggaccgag ggatcctcgg tggtgatcag cgtcgggtgc
      601 acgaaccagc cctcgcggcc gtcgcagatc ccacccgcga tgaccttcat gcccgaggtc
      661 tcgcgcgcct cggagatcgc ggcctggtgc ttgtcgaacg cggtctgctt gatcaccgcg
      721 cccatgaagt tcgagaagtt cgagacgtcg ccgatcttga gcgaccggat cgtgtcgagc
      781 agcggctcgc gcatccggtc ccacaccgac ttcgcgacgt agacccgcga cgcggccgaa
      841 cacttctggc cctggaactc gtagccgccg cgcacgatcg ccgtcgcgag cgcctgcgga
      901 tcggcggacg ggtgggcgag gatgaagtcc ttgccgccgg tctcgccgac cagccgcggg
      961 tagctccggt agcggtcgat gttctcggcg acccgcttcc acatcatgcg gaacacgtcg
     1021 gtcgagccgg tgaagtggat gccggcgagc atcgggtgat cgagcacggc gttggtgctg
     1081 ccgtgcggca ggccggggat gaagttgatg acgccgggcg gcatcccggc ctcctcgagc
     1141 agctggtaga cctgccaggc cgcgagcagc tgggtctcgg cgggcttcca caccacggtg
     1201 ttacccatca gcgccggcgc ggccggcagg ttgccggaga tcgcggtgaa gttgaacggg
     1261 gtcagcgcga acacgaagcc ttcgagcggc cggaggtcgg tcatgttcca cacgccgtgc
     1321 gtcgacaccg gctggacccg gtagagctcg gcggcgaact ggacgttgaa ccgccagaag
     1381 tcgatcagct cgcacgccga gtcgatctcc gcctggtgcg cggtcttgga ctggccgtgc
     1441 atcgtggcgg cgttgagccg catccgccac gtcgtcgaca gcagctcggc ggcgcgcagc
     1501 agcaccgcgg cgcgcgcctc ccagcgcatc gcggcccact cgcggcgggc gtcgagcgcg
     1561 gcgcggatcg cgcgctcggc gacgtcctcg gtggcggcgt ggaaccgcgc gaccacgtgc
     1621 ttgtggcggt gcggcatcac gacgtcgcgg agcttgccgg tgtggacgcg ctggccgccg
     1681 atcacgcacg ggatctcgac gacctcgcgc tcgatcgcgg cgagcgcggc cagaagccgc
     1741 gtgcgctcgg ggctgcccgc cgcgtatccg aggatcggct cgttgacggc gggcgggacg
     1801 gtgactgtcg cgtcgatcat ggcgcacgtg taccacggcg cgcgacgtcg tgatcgtcac
     1861 ggcggaccgc gtcatcggcg tccggaactg ccgatccggc gtgcgcgagc gatacagtgc
     1921 gggcatgcgc tggaccctcg cgtgcctgct cgccgccggc tgcggccggc tcggcttcga
     1981 cagccgggtc cagccggccg gcgatgacac ggtcatgccc gacggcgccg cgaacctggt
     2041 atttgtcacc tcgcagcacg tggtcgtggg ccagctcggc accctcgccg ccgccgacgc
     2101 ggtgtgcaag caggccgcgg ccggggccgg cctgccgggc aactacgtcg cgtggctgtc
     2161 gaccagcaag accagcgcga tcgaccggct ggccggtgcg cgcggctggg tccggcccga
     2221 cgggcgaccg ttcgccgata cgccggccga catcgccgcc ggtcggatct ggtacccggc
     2281 gtcgatcgac gagaacggcg cggtcgcggc cacgctggtc gtcaccacca gcgatgccca
     2341 cggccggttc gacggcatga gctgcaacga tctgaccgac gactcgatcg acgggtcgtg
     2401 cgacttcggc gagtcgaccg ggacggccgg cacgttctcg agctacccgc tgctcggcgg
     2461 gctgtgcttc gtcgatgagc cgatgtactg cttcgcgacc gatcgcaacg tccgggtaca
     2521 ggcgccgcgg cccacgaccg gcgtgcgggt gttcccggcc gagggcttca cgccgggtgg
     2581 cgggctcgcc agcgcagacg cgctgtgcgc acggaccgcc gcggcggctg gcct
//
LOCUS       DASVOR010001737         3054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_279980,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001737 DASVOR010000000
VERSION     DASVOR010001737.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_279980"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF516_32345"
     CDS             <1..362
                     /locus_tag="VF516_32345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112481.1"
                     /translation="AGRRERGPLGVSAEQIAGSQKGRGPSPIRAIARGGEGHRALWIY
                     RDGRELVAQCPGHPACQSSGDALSLVLDSAPIGNYQVIGLSSAAALPPATGSLERDLG
                     AAIAAGATWQPQELEVR"
     gene            complement(365..2614)
                     /locus_tag="VF516_32350"
     CDS             complement(365..2614)
                     /locus_tag="VF516_32350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEX8112482.1"
                     /translation="MPAPPPDARATAPDAGPAADVTLDGTIERFVFRNEESAFTVARL
                     VAADHTMVTIVGELVGVSEGVPLRLHGRWVDDRKFGRQFRVATYQLRAPETLIGIERF
                     LGSGIIPGIGPELARRLVSHFGMDTLEVIDRTPERLVEVAGIGAARAAKLAAAFAAQR
                     HVQDVMVFLRGHGVSAAFAARIVKKYGKDAINVVRANPYRLAHEIWGIGFRTADAIAE
                     KLGIARDAPERLEAGLLHALETSAEDGHMHLPDDELIAAAAELLGVAADRLAPRLAAL
                     EQSRLIVRETLGHRGACSMLPWAHDAEATAAALLVQLIRTPARGLALDIHAAIHAFEA
                     VTGVQLAAQQRRAVEAALVDKCTVITGGPGVGKTTIVKAIVHLAKLVHRRVALAAPTG
                     RAAKRLGEATVMEAMTLHRLLEFQPHEGGFQRGPENPLDADLLVVDEASMVDAQLFSA
                     VVAALRPGAQLVLVGDIDQLPSVGAGAVLSDVIDSEAATVIRLTEIFRQAAASQIVVS
                     AHAINAGELPVLDPPAPAGAPGTPAPSDFYFIARDDAEAARATIVELVAERIPARFGF
                     DPIADIQVLAPMHRGELGTSALNRALQDRLNPSGAGGSELARGERVFRRGDKVMQLRN
                     DYDKSVFNGDIGVIAAIDGEGGVISVEFDGRLASYERSELDQLAHAYAVSVHKSQGSE
                     YAAVVIPLVTQHYMMLQRSLLYTAVTRGKRLVVIVGSKRAVALAVRNADARRRYTWLA
                     ERVRAQMAG"
     gene            complement(2694..>3054)
                     /locus_tag="VF516_32355"
     CDS             complement(2694..>3054)
                     /locus_tag="VF516_32355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112483.1"
                     /translation="RRRRAPRRCGMTRRPAPPPSGARSIWLAALLGLALGVGLPAGCV
                     VRRFTGPEFTGTCDGACAHYMQCKPGHASADRDRCLAECPDVFSDRDSLMQYESLACQ
                     DAVEYIDGSTARTAAHR"
BASE COUNT          391 a         1122 c         1146 g          395 t
ORIGIN      
        1 gggcgggccg ccgcgagcgg gggccgctgg gggtgtcggc cgagcagatc gccggctcgc
       61 agaaggggcg cggcccgtcg cccatccgcg cgatcgcgcg cggcggcgag ggtcaccgcg
      121 cgctctggat ctaccgcgac gggcgcgaac tcgtcgcgca gtgcccgggt cacccggcgt
      181 gccagagctc cggcgacgcg ctgagcctcg tgctggacag tgcgccgatc ggcaactacc
      241 aggtcatcgg gctgagctcg gcggcggcgc tgccgccggc caccggctcg ctcgagcgcg
      301 acctcggggc tgcgatcgct gcgggggcga cctggcagcc gcaggagctg gaggtgaggt
      361 agcgtcagcc tgccatctgg gcccgcaccc gctcggcgag ccaggtgtag cgccgccggg
      421 cgtcggcgtt gcgcaccgcg agcgccaccg cgcgcttgct gccgacgatc acgaccagcc
      481 gcttgccgcg ggtgaccgcg gtgtacagca ggctgcgctg cagcatcatg tagtgctggg
      541 tcaccagcgg gatcacgacc gcggcgtact ccgagccctg gctcttgtgg acgctcaccg
      601 cgtaggcgtg ggcgagctga tcgagctcgc tgcgctcgta gctcgcgagc cggccgtcga
      661 actcgacgct gatcacgccg ccctcgccgt cgatcgccgc gatcacgccg atgtcgccgt
      721 tgaacacgga cttgtcgtag tcgttgcgca gctgcatcac cttgtcgccg cggcggaaca
      781 cccgctcgcc gcgcgccagc tccgagccgc cggcgccgct cgggttcagt cggtcctgca
      841 gcgcgcggtt gagcgccgag gtcccgagct cgccgcggtg catcggcgcg agcacctgga
      901 tgtcggcgat cggatcgaac ccgaaccgcg ccgggatccg ctcggcgacc agctcgacga
      961 tcgtggcgcg cgcggcctcg gcgtcgtcgc gcgcgatgaa gtagaagtcg gacggcgcgg
     1021 gggtgcccgg agcgccggcg ggcgccggcg gatcgagcac cggcagctcg ccggcgttga
     1081 tcgcgtgggc cgagaccacg atctggctcg ccgcggcctg gcggaagatc tcggtcagcc
     1141 ggatcacggt cgcggcctcg gagtcgatca cgtcggacag caccgcgccg gcgccgaccg
     1201 acggcagctg atcgatgtcg ccgaccagca ccagctgcgc gcccggccgc agcgccgcga
     1261 ccaccgcgct gaacagctgg gcgtcgacca tcgaggcctc gtcgaccacc agcagatcgg
     1321 cgtcgagcgg gttctccggg ccgcgctgga acccgccctc gtggggctgg aactcgagca
     1381 gccggtgcag cgtcatcgcc tccatcaccg tggcctcgcc gagccgcttg gcggcgcggc
     1441 cggtcggcgc ggcgagcgcg acccggcggt ggaccagctt cgcgaggtgg acgatcgcct
     1501 tgacgatcgt ggtcttgccg acgccggggc cgccggtgat caccgtgcac ttgtcgacca
     1561 gcgcggcctc gacggcgcgg cgctgctggg ccgcgagctg gacgccggtg accgcctcga
     1621 acgcgtggat cgcggcgtgg atgtcgagcg ccaggccgcg cgccggggtc cggatcagct
     1681 ggacgagcag ggcggccgcg gtggcctcgg cgtcgtgcgc ccacggcagc atcgagcacg
     1741 cgccgcggtg gccgagcgtc tcgcgcacga tcaggcggct ctgctccagc gcggcgagcc
     1801 gcggcgccag ccggtcggcg gcgaccccga gcagctcggc ggcggcggcg atcagctcgt
     1861 cgtcgggcag gtgcatgtgg ccgtcctccg cgctggtctc cagcgcgtgg agcaggccgg
     1921 cctccagccg ctccggtgcg tcgcgcgcga tcccgagctt ctcggcgatc gcgtccgcgg
     1981 tgcgaaaccc gatgccccag atctcgtggg cgagccggta cgggttggcg cgcaccacgt
     2041 tgatcgcgtc cttgccgtac ttcttgacga tccgcgcggc gaacgccgcc gacacgccgt
     2101 ggccgcgcag gaacaccatg acgtcctgga cgtggcgctg ggccgcgaac gcggcggcga
     2161 gcttggccgc gcgcgccgcg ccgatcccgg cgacctcgac cagccgctcc ggcgtgcgat
     2221 cgatgacctc cagcgtgtcc atcccgaaat ggctcaccag ccggcgcgcg agctcgggcc
     2281 cgatccccgg gatgatcccc gagccgagga agcgctcgat gccgatcagc gtctcgggcg
     2341 cgcgcagctg gtaggtcgcg acgcggaact ggcgcccgaa cttgcgatcg tcgacccagc
     2401 ggccgtgcag ccgcagcggc acgccctcgc tgaccccgac cagctcgccg acgatcgtca
     2461 ccatcgtgtg gtcggcggcg accagccgcg cgaccgtgaa cgccgactcc tcgttgcgga
     2521 acacgaaccg ctcgatcgtc ccgtcgagcg tgacgtcggc cgccggcccc gcgtcggggg
     2581 cggtcgcacg agcgtcaggc ggcggagcgg gcacgcctgc attatgcccg gcagggcccg
     2641 cgcggcgaat cggcggatgt cgctacgagc ttgcgacgtg cgatcgcgcg gtgtcagcgg
     2701 tgcgcggcgg tgcgcgccgt gctgccgtcg atgtactcga cggcgtcctg gcacgcgagg
     2761 ctctcgtact gcatcagcga gtcgcgatcg ctgaacacgt cggggcactc cgcgaggcag
     2821 cgatcgcgat cggccgaagc atggcccggc ttgcactgca tgtagtgcgc gcacgcgccg
     2881 tcgcaggtcc cggtgaactc cgggccggtg aaccggcgga ccacgcagcc cgcgggcagc
     2941 cccacgccga gcgcgaggcc gagcagggcg gccagccaga tcgaccgcgc tcccgagggc
     3001 ggcggcgccg gccggcgggt cataccgcac ctccgtggcg cccggcgccg gcgg
//
LOCUS       DASVOR010001738         3300 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_397130,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001738 DASVOR010000000
VERSION     DASVOR010001738.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397130"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            710..1081
                     /locus_tag="VF516_32360"
     CDS             710..1081
                     /locus_tag="VF516_32360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8112484.1"
                     /translation="MFIAYDVALDLVRALRPVVAQLRTYSPEAADQVERAASSIVLNL
                     AEGGRRYGRDPRRFWDMAHGSAGEIRGALDLAEAWGWEVESAHARALLDRELGLLWGL
                     TRRSRTKDASRPASGAKTRPR"
     gene            complement(1465..1782)
                     /locus_tag="VF516_32365"
     CDS             complement(1465..1782)
                     /locus_tag="VF516_32365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112485.1"
                     /translation="MLEALDPSEPVEVASEVEEHHELVVGTGLDHAQQLGGLAGADRA
                     GHEDARVARKRRGQELADLARGAQGRSARQCEDVTYSYTSKRWYSVCQVSRRDMAARY
                     HAG"
     gene            2168..2362
                     /locus_tag="VF516_32370"
     CDS             2168..2362
                     /locus_tag="VF516_32370"
                     /inference="COORDINATES: protein motif:HMM:NF024767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8112486.1"
                     /translation="MPTRRELAKVLAAKGDLTGAIENLQRALSTQRRIFERGDHPDIA
                     ATMCELERLQALQRDVQRPD"
     gene            complement(2403..>3300)
                     /locus_tag="VF516_32375"
     CDS             complement(2403..>3300)
                     /locus_tag="VF516_32375"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HEX8112487.1"
                     /translation="HRELIKLKGTYLDALPPLVNPATGRVHTTFNQVVAETGRLSSQD
                     PNLQNIPIRSELGRQIRRGFVAAPGHVLIAADYSQIELRILAHLSGDALLGSAFRDHI
                     DVHTQTAAEVFGVPRDQVTPEQRRIAKAVNYGLSYGQSDFGLARALDVPRTQAAEYSR
                     RYFQRFPTIHKFMEDIVTLARAQGGARTLLGRWRPIPNLMSKSPQARHAAERVAQNTP
                     MQGAGADIIKLAMLATSRRIAREKWPVKMLLTVHDELVFESPPAIAEAVGAALKQEME
                     AVYQLSVPLEVDIGIADNWADA"
BASE COUNT          484 a         1169 c         1160 g          487 t
ORIGIN      
        1 gtcgatccac cgcccacctc tcgcatcgcg aatccctccg ccgaccaccg ttaccgaacg
       61 ccgttaccga acgccgaacg ccgttaccga cactgcccac cggtcgccgt cgcccccacc
      121 cgatcggcgc gattccgcgg aatcgccgtc ggcgcgctgg caattgacac gcgctgtgga
      181 tgcttgtccc ggttcgatgc gattccgcgt ccgtagtcgg cggaagcgat cggtagcggc
      241 ggtcggccgt cggcaacggc ggtcggcggt cggcaacggc cgtcggtagc ggcggtcggc
      301 ggtcggcaac ggcggtcggt agcggcggtc ggtagcggcg gtcggccgtc ggtagcggtg
      361 ttccgtgctc ggcaacggtg gttggccccg gcctggtagt cggtgatcgg cggtcagtac
      421 ggcaccctct cgcgacccgg cacgccggcg cacccagctc accccgcggc gcctcctccc
      481 tccacggctg ggccgccctg cgcccgtccg cgcacgcccc gtgccgaacg ccgccgcgtc
      541 cgggtggctc ccaccaccgc cgcgaagacc ggcgccagag aagaccacgt acctcggtga
      601 cggcgttcgt cacgggtcct gcgcgggtcg gtcgcgacgg gcgtcgccca gccgcggagg
      661 tcgtcagcag agggcgttcc ccttccacag atgaatgccg agggaaacca tgttcattgc
      721 ctatgacgtc gcgctggatc tggtccgtgc tctgcgtccc gtggtcgcgc agcttcggac
      781 gtacagcccc gaggccgccg atcaggtcga gcgcgcagcc agcagcatcg tgctcaacct
      841 cgccgagggc ggtcgccgct atggccgcga tccacggcgg ttctgggaca tggcgcacgg
      901 cagcgccggg gagatccgtg gtgcgctcga tctggcagaa gcctggggct gggaggtcga
      961 gagcgcgcat gcacgtgcgc tgctcgatcg cgagctcggg ctgctgtggg gactcacccg
     1021 ccgcagccgc accaaagacg catcgcgccc agcgtcagga gccaagacgc gaccgcggtg
     1081 acccgctcgc gaaccagcac ccagcgcggt ggatggaccg cttccgaaag tccgccgaac
     1141 cacgccagcc tcacctgccc ggaagcgatt ccgcggaatc gtcctggacc gagccccgct
     1201 cggcccggca cgcgtcacat cggccgaccg ccagcattcg atcgacgatt ccgcggaatc
     1261 gtccggaccg agccccgccc ggcccagcgc ccattggcat cggcgaaccg ccggcgttcg
     1321 atcgacgatt ccgcggaatc gcggcgtggc cggaacacag acccggacac cgtggagcgg
     1381 agggtagcgc gtccccttgg cgctggcgtc ccgcgattcc gacggtggcg actcgagcgc
     1441 ggcggcgtga accgtcggca acccctaccc ggcgtggtac cgtgcggcca tgtctcggcg
     1501 cgagacctgg caaacgctgt accagcgctt tgacgtgtac gaatacgtca cgtcctcgca
     1561 ctggcgcgcc gaccgcccct gagccccgcg cgcaagatca gcgagctcct ggccacgccg
     1621 tttgcgagcg acgcgcgcat cctcgtgacc ggcacgatcg gcaccggcaa gaccaccgag
     1681 ctgttgcgcg tggtcgaggc ccgtgcccac cacgagttcg tgatgttcct cgacctcgct
     1741 cgccacttcg accggctcgg acggatcgag agcctccagc acgtgaacca gtgggaggtc
     1801 tgcttcctgg tcgggctcgc gctctaccgc gccgcggagg acaccctcgg attccccttc
     1861 ccgaagggct tattgaagga cctcgaagcg gcgtggaccg agctggcgca caaatccggc
     1921 accccgcagc cgacgcaggt cgatctggaa gacgggcgac ctggctcggg ctcgcgctca
     1981 cctggaacgt gcgcttgcca tccaggagct cgcctttcga tccgatcatc cggacacggc
     2041 gcgcacgctc gtccggctgg ccgctgtact cgcggatgcc ggcgatctgg atggcgcgca
     2101 ggcaacgctc ggacaagcac tcgggatcca gtgccgggta ttcggcgacg atggccagtt
     2161 cgccggcgtg ccgacccggc gagagctcgc gaaggtcctc gccgccaagg gcgatctcac
     2221 cggggcaatc gagaacctgc agcgggcgct gagcacacag cgccggatct tcgagcgcgg
     2281 tgatcacccg gacatcgcag ccacgatgtg cgagctggag cggctgcagg cgctgcagcg
     2341 cgacgtgcag cgcccggact gacgtcgcgc ccggccacgc gtcggcccag ccaccgacgc
     2401 gtttacgcgt cggcccaatt gtccgcgatg ccgatgtcga cctcgagcgg cacgctgagc
     2461 tggtagacgg cctccatctc ctgcttgagc gccgcgccga ccgcctcggc gatggcgggc
     2521 ggcgactcga acaccagctc gtcgtgcacg gtgagcagca tcttgaccgg ccacttctcg
     2581 cgggcgatcc ggcgtgaggt cgccagcatc gcgagcttga tgatgtcagc gccggctccc
     2641 tgcatcggcg tgttctgcgc gacccgctcg gcggcgtgcc gggcctgcgg cgacttcgac
     2701 atgaggttgg ggatcggccg ccaccggccg agcagcgtgc gcgccccgcc ctgcgcccgg
     2761 gccagcgtga cgatgtcctc catgaacttg tggatcgtgg ggaagcgctg gaagtagcgc
     2821 cgcgagtact cggcggcctg ggtccggggg acgtcgagcg cgcgcgccag gccgaagtcg
     2881 gactggccgt aggacaggcc gtagttgacc gccttggcga tccggcgctg ctcgggggtg
     2941 acctgatcgc gcggcacgcc gaacacctcg gccgcggtct gggtgtggac gtcgatgtga
     3001 tcgcggaacg ccgagccgag cagggcatcg cccgacaggt gggcgaggat ccgcagctcg
     3061 atctgcgagt agtcggccgc gatcagcacg tggccgggcg cggcgacgaa gccgcggcgg
     3121 atctggcggc cgagctcgct gcggatcggg atgttctgca ggttggggtc ctgcgacgac
     3181 agccggccgg tctcggcgac gacctggttg aacgtggtgt ggacccggcc ggtggcgggg
     3241 ttgaccagcg gcggcagcgc gtcgaggtag gtgcccttga gcttgatcag ctcgcggtgg
//
LOCUS       DASVOR010001739         4201 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_76249,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001739 DASVOR010000000
VERSION     DASVOR010001739.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4201
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76249"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..352
                     /locus_tag="VF516_32380"
     CDS             161..352
                     /locus_tag="VF516_32380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112488.1"
                     /translation="MSHHGYGAATRLHNKYGKDIECHASLIDEKSKKLKRSAPRSAPG
                     WEAEREVVRLREELERLKR"
     gene            complement(419..1483)
                     /locus_tag="VF516_32385"
     CDS             complement(419..1483)
                     /locus_tag="VF516_32385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112489.1"
                     /translation="MRRWTFAAIAGLLSVAGRDALADDPPRDITRLDLSVPDLPALVA
                     LNLSSDKVTRPSTPQDFLASIANGFSTDGSYHDGVAIEVSPFGLAGMTSPYATALRVS
                     IATTAITSNGKTGGKAATGLRWSVGAYDPTTDAALIHCLADHLPHMAPPPVEGPLPPS
                     GVSQPKDEAAKRAIDQELQACRDKARAGHLAQTALETAVVLDGTSTDSLKLTDLGTPH
                     TSAWLVASWGWNSWDGKLDVPRHPLGIQPTGMLRFDLTNVMNGRRKDLFAGARLPFVW
                     DSFGLFAEAGITLGDLSKVQSTSTTKTMRLGGGFDYRIADGSWLGVYIGQDFGDGAAG
                     FSLLSNVKFQFGEQRQYGLR"
     gene            complement(1480..1920)
                     /locus_tag="VF516_32390"
     CDS             complement(1480..1920)
                     /locus_tag="VF516_32390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112490.1"
                     /translation="MSDLWPLLRLERQDGSQKGVDMSDPVIKVAAGQPVTVQMSFQGI
                     ANHYEAYLYEPLPGDKWRRVQTLESDGSSDDARPDEFTFTAPAAGKSLLFYFQSPMTA
                     AAVPAEVGATATVQQAGKRIDAVSVSGPVTTFVNVVLRLQVVAQ"
     gene            complement(2017..3471)
                     /locus_tag="VF516_32395"
     CDS             complement(2017..3471)
                     /locus_tag="VF516_32395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112491.1"
                     /translation="MRKWWLATVLLTGCHGAPPPPPAPPATVAPAEPSDGARSGTRLK
                     LLLWQSGDGFTSWTGWFFDTQLAAPCRAVAWTDGRLYCTPIDSPVPLGVFDSTPAPDR
                     DRTLIGYTDAGCTQPEVIAYDAPSLYAAALVDDGCSPDGVHPSWKVSHLYRRGAKFGT
                     PDSYFTNEVTVGVCESWTGFLSTAYAAEPGPSPSDLVLLSLRTAPTTDRLTTTYLVSE
                     DGLQFPAYAHDTQFDADCYFASPLGGAPTCVPVGAVSSLRGGADGLDASCTKPLATVP
                     ARCPRPSAVFTTFVNPSDFDPTKFLPVGAPAAELCAQTPPAADTSYYQLGAAVPLVTA
                     SLERENITGRRLELYHRVAGSWTGRDPDHLHDNHYDLDCRLHADGLTSAYCSVYPACG
                     SPLSGYSDPSCKTQVTWFDSTGCSSPPPPLFDRLTVLTDQRETNPVYLAVPIEAGGPA
                     YRCDVLEGHTLYRTAPDPIPLDRFEQFAPIVDSR"
     gene            complement(3488..>4201)
                     /locus_tag="VF516_32400"
     CDS             complement(3488..>4201)
                     /locus_tag="VF516_32400"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8112492.1"
                     /translation="VTAIAAFDDGSTRDVTGQAMWSSSATGVATVSAGWVSGTAPGSA
                     QISASYSGSTGAATIAVVPGLQSITVTPRASVPKGLAVQMTAIGAYADGTSRDLTSRA
                     TWTSSDPLRGAVRPDGQVSTLAVGAVDITAHFAALTGAATVAVMPAAAVQVSVSPAGG
                     FLHVGQTLQLTAVATMTDGTVLDVTGQATWASRDTAIATVSSTGRVTAVAPGAPSTYV
                     PIEATYQGIPSDSAITVGP"
BASE COUNT          651 a         1386 c         1545 g          619 t
ORIGIN      
        1 cttcgtgccc aggccaaggc ggcgctgacc ggcctgggct ggaagcctgc gatcgcgcac
       61 gccgcggtcg ctgccgcggc agaggcgcag ggggacacgc cgacgctgga gcgcatgatc
      121 tttgaagcat tgcggcgatg ctccacgccg aaggtgtgag gtgagccatc atgggtacgg
      181 tgcagccacg cggttacaca acaagtacgg caaggacatc gagtgccacg cgtcgctgat
      241 cgacgagaag tccaagaagc tgaagcgctc agcgccgagg agcgcgcccg ggtgggaggc
      301 cgagcgcgag gtcgtacggc tgcgcgagga gctggagcga ctgaagcggt gagccgagct
      361 gcccgtgcct ggcgccgcgc cgcgttcgcc tcgctcgaga tccgagcccg gcgctgtctc
      421 agcggaggcc gtactggcgt tgctccccga actggaactt cacgttcgac agcaggctga
      481 agcccgcggc gccgtcgccg aagtcctggc cgatgtagac gcccagccag ctgccgtcgg
      541 cgatgcgata gtcgaagccg cctcccagcc gcatggtctt cgtggtggag gtcgactgca
      601 ccttggacag gtcgcccagc gtgatgccgg cctcggcgaa caggccgaac gagtcccaca
      661 cgaaggggag ccgggctccg gcgaacaggt ccttcctgcg tccgttcatc acgttggtga
      721 ggtcgaagcg cagcatcccg gtcggctgga tgccgagcgg atgcctgggg acgtccagct
      781 tgccatccca ggagttccac ccccagctcg cgacgagcca cgcgctcgtg tgaggcgtgc
      841 cgaggtcggt gagcttgagg ctgtcggtcg aggtgccgtc gagcacgacc gcggtttcca
      901 gcgcggtttg tgcgaggtgc ccggcgcgcg ccttgtcgcg gcacgcctgc agctcctgat
      961 cgatcgcccg cttggccgcc tcgtccttgg gctgtgagac ccccgatgga ggcagcgggc
     1021 cttcgacggg agggggcgcc atgtgcggta ggtggtccgc caggcaatgg atcagcgccg
     1081 cgtcggtggt cgggtcgtac gcgccgacgc tccagcgcag cccggtggcc gccttcccgc
     1141 cggtcttgcc gttgctggtg atcgccgtcg tggcgatcga gacccgcagc gcggtcgcgt
     1201 acggcgacgt catgccggcg agaccgaacg gtgatacctc gatcgcgaca ccgtcgtgat
     1261 acgagccatc cgtcgagaat ccgttcgcga tcgaggcgag gaagtcctgc ggtgtgctcg
     1321 gccgggtcac cttgtcgctg gacaggttga gggcgaccag ggccgggaga tcgggaacgg
     1381 agagatcgag ccgggtgatg tcccgcggcg gatcgtctgc gagcgcgtcc cgtccggcga
     1441 ccgacagcaa cccggcgatc gcggcgaagg tccagcgcct cattgcgcca ccacctgcag
     1501 gcgcaggacg acgttcacga acgtcgtgac cggcccggac acggacacgg cgtcgatccg
     1561 cttgccggcc tgctggacgg tggccgtggc tccgacctcc gccgggacgg cggccgcggt
     1621 catcggcgac tggaagtaga acagcagcga cttcccggcc gccggcgccg tgaaggtgaa
     1681 ctcgtccggg cgagcgtcgt ccgacgatcc gtcgctctcc agcgtctgga cccgcctcca
     1741 cttgtcgccc ggcaacggtt cgtagagata ggcctcgtaa tgattggcga tgccctggaa
     1801 agacatctgc accgtgactg gctggccagc agctaccttg atgaccggat ccgacatgtc
     1861 cacccccttc tgagagccgt cttgtcgctc taggcgaagc agcggccaca gatccgacat
     1921 gccgtatgtc gatatctgcc gggcgacggc gaccggccgc tgcggacata attgtccggc
     1981 cgcggtggtc cggagcgggc gctccggaac gggcggtcac cgggagtcga cgatgggcgc
     2041 gaactgctcg aagcggtcga gcgggatcgg gtcgggcgcg gtccgataca gcgtgtgccc
     2101 ttcgaggacg tcgcagcgat acgccgggcc gcccgcctcg atcgggacgg cgaggtagac
     2161 cgggttggtc tctctctgat ccgtgaggac ggtgaggcga tcgaacagcg gcgggggcgg
     2221 agacgagcag ccggtgctat cgaaccacgt cacctgggtc ttgcaactcg gatcggagta
     2281 ccccgacagc gggctaccgc acgccggata gacactgcag taggcggagg tcaatccgtc
     2341 ggcgtgcagc cggcaatcga gatcgtagtg gttgtcgtgg aggtggtcgg ggtcgcggcc
     2401 ggtccacgag ccggcgacgc ggtggtacag ctcgagccgg cggccggtga tgttctcgcg
     2461 ctccaggctc gcggtcacga gcggcaccgc ggcaccgagc tggtagtagc tggtatccgc
     2521 cgcaggcggc gtctgcgcgc acagctcggc ggccggtgcg ccgaccggga ggaacttcgt
     2581 cgggtcgaag tcgctgggat tgacgaaggt ggtgaacacc gccgatggcc gcggacagcg
     2641 cgcggggacg gtggcaagcg gcttggtgca cgaggcgtcg aggccatcgg caccgccgcg
     2701 gagcgacgag accgctccga ccgggacgca ggtcggagcg ccgccaagcg gactggcaaa
     2761 atagcaatcg gcatcgaact gcgtgtcatg ggcatacgcc gggaactgca ggccatcctc
     2821 ggacaccagg tacgtcgtgg tcagccgatc ggtcgtcgga gccgtgcgca gcgagagcag
     2881 gacgaggtcg gagggcgagg gccctggctc cgccgcgtac gcggtggaca ggaaccccgt
     2941 ccagctctcg cagaccccga cggtcacttc gttcgtgaag tacgagtccg gggttccaaa
     3001 cttcgccccc cgccggtaca ggtggctgac cttccaagac ggatggaccc cgtcgggcga
     3061 gcagccgtcg tcgaccagcg cggcggcgta cagcgatggc gcgtcgtagg cgatcacctc
     3121 tggctgggtg caaccggcat cggtgtagcc gatcaacgtc ctgtcgcggt cgggggcggg
     3181 agtggagtcg aacaccccga gcggaaccgg tgaatcgatc ggtgtgcagt acaggcggcc
     3241 gtcggtccat gccacggctc ggcacggggc cgccagctgg gtatcgaaga accagccggt
     3301 ccacgaggtg aagccgtcgc cgctctgcca cagcaggagc ttgagccgtg ttccgctgcg
     3361 cgcgccgtcg gacggctcgg cgggcgcgac cgtcgccggt ggcgccggcg gcggcggggg
     3421 agcgccgtga cagccggtca ggaggaccgt ggcaagccac cacttcctca tcgctgcacc
     3481 tccatcgtca cggccccacc gtgatggcgg aatcgctcgg gatgccctgg tacgttgcct
     3541 cgatcggcac gtacgtcgag ggcgcgccgg gggccacggc ggtgacccgg ccggtggagg
     3601 ataccgtcgc gatcgccgtg tcgcgcgacg cccacgtcgc ctgcccggtg acgtcgagca
     3661 cggtgccgtc ggtcatcgtc gccaccgcgg tcagctgcaa ggtctggccg acgtggagaa
     3721 aaccgcccgc cggagagacg ctcacctgca cggcggcggc gggcatcacc gccacggtcg
     3781 ccgcgccggt gagcgccgcg aagtgggcgg tgatgtcgac cgcgccgacg gcgagcgtgg
     3841 agacctgccc gtcgggccgc acggcaccgc gcagcggatc gctgctggtc cacgtcgccc
     3901 gcgaggtgag gtcgcgcgag gtcccgtcgg cgtaggcccc tatcgcggtc atctgcacgg
     3961 ccaggccctt gggcacgctc gcgcgcgggg tgaccgtgat cgactgcagg cccggaacga
     4021 ccgcgatcgt cgcagcgccc gtggaccccg agtagctcgc cgagatctgt gccgatcctg
     4081 gagcggtccc ggagacccag ccggcgctca cggtcgcgac gccggtggcg ctcgacgacc
     4141 acatcgcctg ccctgtgacg tcgcgcgtgc ttccgtcgtc gaacgccgcg atcgctgtca
     4201 c
//
LOCUS       DASVOR010001740         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_514746,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001740 DASVOR010000000
VERSION     DASVOR010001740.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514746"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2108)
                     /locus_tag="VF516_32405"
     CDS             complement(<1..>2108)
                     /locus_tag="VF516_32405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HEX8112493.1"
                     /translation="GSVARDVMPVFRQVYVRMRRVPPSAWERTLYVIRKLAENRVRDS
                     GVDPGGYFHVASLSTETIVYKGLLLPRQLPLFYADLRAPELASAIAVVHSRFSTNTFP
                     TWDLAQPFRYIAHNGEINTLRGNTNWMQARRSQLKSAKFSGGLERLHPIIVPGKSDSA
                     QFDNMVEMLTLGGRTLPHAMMLMIPEAWEDALPGDADDGAPVAAVPGKNTKPIDEDRK
                     SFYRYASSLMEPWDGPAAIVFSDGQLVGATLDRNGLRPARWTITTDDRVILASETGVI
                     DVPPERVRAKGRLQPGMMFVVDTSEGRIVDDAELKRDVASRFPYKKWLDKNVFELERL
                     EGGPPPPRITGDELMRLARAHGYTDECVGVLVEAMARDGKEPIGSMGTDTPLAVLSDQ
                     APNLSAYFHQLFAQVTNPPIDPIREALVMSLETTMGPDGNTFDETPEQCHQLRLRGPV
                     IGTRDLARIRAVREGVFDPVTLPLLWPIVDGPDGLAAAVDRLCRAAAAAIDDGHNLLI
                     LSDRGVDARHVAIPSLLALSAVQHHLVTAGIRMQAGLVVETAEAREVHDIALLIGYGA
                     AAVNPYLALDLVRDLVARGRVPGTVDEAVARYVHALEDGLRKVMSKMGISTIQSYRGA
                     QIFEAVGLDRDLIDRHFTGTPSRLGGIGLHELGGTAQPRDRTRQPGLAGGHGIQHRQR
                     DRLEHALVRVLDPREVGVID"
BASE COUNT          289 a          752 c          753 g          314 t
ORIGIN      
        1 gatcgataac gccgacctcg cgcggatcaa gaacgcgcac gagggcgtgt tcgagccggt
       61 cacgctgtcg atgctgtatc ccgtggccgc cggcgagtcc gggctggcga gtgcgatcgc
      121 gcggctgcgc cgtgccgccg agctcgtgca ggccgatccc gccgagccgc gacggtgtcc
      181 cggtgaagtg gcgatcgatc aggtcgcgat cgagcccgac cgcctcgaag atctgcgcgc
      241 cgcggtagct ctggatcgtc gagatcccca tcttcgacat caccttgcgc agcccgtcct
      301 cgagcgcgtg gacgtagcgc gcgaccgcct cgtcgaccgt ccccggcacc cgcccgcgcg
      361 cgaccaggtc gcggacgagg tcgagcgcga ggtacgggtt gaccgcggcg gcgccgtagc
      421 cgatcaggag cgcgatgtcg tggacctcgc gcgcctcggc ggtctcgacc accaggccgg
      481 cctgcatccg gatcccggcg gtcaccaggt ggtgctgcac cgccgacagc gcgagcagcg
      541 acgggatcgc gacgtggcgc gcgtcgaccc cgcgatccga caggatcagc aggttgtggc
      601 cgtcgtcgat cgcggcggcc gcggcgcggc acagccggtc gaccgccgcg gccaggccgt
      661 ccggcccgtc gacgatcggc cacagtagcg gcagcgtcac cggatcgaac acgccctcgc
      721 gcaccgcgcg gatccgggcg aggtcgcggg tgccgatcac cgggccgcgc agccgcagct
      781 ggtggcactg ctccggggtc tcgtcgaacg tgttgccgtc gggacccatc gtggtctcga
      841 gcgacatcac cagcgcctcg cggatcggat cgatcggcgg gttggtgacc tgcgcgaaca
      901 gctggtggaa gtacgcggac aggttgggcg cctgatccga cagcaccgcg agcggcgtgt
      961 cggtgcccat cgagccgatc ggctccttgc cgtcgcgcgc catcgcctcg accagcacgc
     1021 cgacgcactc gtcggtgtag ccgtgggcgc gggcgagccg catcagctcg tcgccggtga
     1081 tccgtggcgg cggcgggccg ccctcgagcc tctcgagctc gaacacgttc ttgtcgagcc
     1141 acttcttgta cgggaaccgg ctggcgacgt cgcgcttgag ctcggcgtcg tcgacgatcc
     1201 ggccctcgga cgtgtcgacc acgaacatca tgccgggctg cagccggccc ttggcgcgca
     1261 cccgctcggg cggcacgtcg atcacgccgg tctccgacgc caggatcacg cggtcgtcgg
     1321 tggtgatcgt ccagcgcgcg ggccgcaggc cgttgcggtc gagcgtcgcg ccgaccagct
     1381 ggccatcgct gaacacgatc gccgccgggc cgtcccacgg ctccatcagc gacgacgcgt
     1441 agcggtagaa gctcttgcgg tcctcgtcga tcggcttggt gttcttgccg gggaccgcgg
     1501 cgaccggcgc gccgtcgtcg gcatcgcccg gcagcgcgtc ctcccaggcc tcggggatca
     1561 tcagcatcat cgcgtgcggc agcgtgcggc cgccgagcgt gagcatctcg accatgttgt
     1621 cgaactgcgc cgagtcgctc ttgcccggca cgatgatcgg gtgcaggcgc tcgagcccgc
     1681 ccgagaactt ggcgctcttg agctggctcc tgcgcgcctg catccagttg gtgttgccgc
     1741 gcagcgtgtt gatctcgccg ttgtgcgcga tgtagcggaa cggctgggcg aggtcccagg
     1801 tcgggaacgt gttggtcgag aaccgcgagt gcaccaccgc gatcgcgctc gccagctcgg
     1861 gcgcccgcag gtcggcgtag aacagcggca gctggcgcgg cagcagcagg cccttgtaga
     1921 cgatggtctc ggtcgacagg ctcgcgacgt ggaagtagcc gcccggatcg acgccgctgt
     1981 cgcgcacccg gttctcggcg agcttgcgga tcacgtacag cgtgcgctcc cacgccgacg
     2041 gcggcacccg ccgcatccgc acgtagacct ggcggaacac cggcatcacg tcgcgcgcga
     2101 ccgagccg
//
LOCUS       DASVOR010001741         3334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_193542,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001741 DASVOR010000000
VERSION     DASVOR010001741.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193542"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VF516_32410"
     CDS             complement(<1..372)
                     /locus_tag="VF516_32410"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8112494.1"
                     /translation="MGCPCCGAAPEVGVLCRRCAREAAPCDGLIPDHLRSTIDASAAQ
                     AWLVDGFGGAHAIGPQCRIGRSHDVDLVVLAVSVSREHAVVIRSDAGWSVRDLGSRNG
                     TFVDGVRCQGRTALSPRSLLKV"
     gene            complement(458..1306)
                     /locus_tag="VF516_32415"
     CDS             complement(458..1306)
                     /locus_tag="VF516_32415"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8112495.1"
                     /translation="MCRSCAKEVSPCEGLIQDHIHSRIDSTDAEAWVVDGFGGAHPIA
                     VRSRIGRNHDNELIVLASSVSREHAELQHADAGWTVTDLGSRNGTFVNGVRVQGAAVL
                     PGRAVLRVGDVAMWFLAEIVSEPVRRPAMTTDGTRGGLVRYQLVRGDKELCIVATDND
                     ASAGALMWREVGADTWSERSLAPLEFQLLRALCIRAHEEASSPSTIRGCVPTKQLVND
                     LPFQSKYANQENVRQVVLRLRGVLTEVGVGGLLAVAPGRGYYLASPVTVGTTLGATSL
                     PEQLRR"
     gene            complement(1716..1862)
                     /locus_tag="VF516_32420"
     CDS             complement(1716..1862)
                     /locus_tag="VF516_32420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112496.1"
                     /translation="MLGTLDLQGVPSLPASYLIQVGGGVTIAGTGKVTRVDTRMPTPA
                     ATSS"
     gene            complement(1948..>3334)
                     /locus_tag="VF516_32425"
     CDS             complement(1948..>3334)
                     /locus_tag="VF516_32425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112497.1"
                     /translation="SWVSWVEASRFDPAVAYAAFDRHTFGDMTPWVFRTTDYGKTWTR
                     IVGPDQGVRGYAHTIKEDTIKKDLLFVGTELGLWISLDGGARWAQFRGGEFPDGVAVR
                     EVQVHPRDGDLVIATHGRGIWIVDDIAPLRALGDDALQKSTAFLPIRDVQQRMQANGG
                     WAEGDATFSGDSAPGGAAITYYLRSRHVYGPIKLEILDAAGKLVDTIAPSKRRGINRI
                     SWAMRVKPPRVPRAAQIAFASSQGPRVPPGTYTVRLTRGAEVVETKLAIGLDRRAPYN
                     AVDRKAQFDAVMKAHGVFGDMSKLTTRIDMAREAVRDRLKGLPDADPLAGKLHALGDR
                     LEAARKLIVATKEGGAITGEERIREHLDSLYGALNGWEGRPAKYQLDAIEALRRELGD
                     VAATVDTIVGKDARALDDELKQHQLAPLPQISALDRVGGLDALALHCVATAGRDCGGD
                     ERAAAASQERD"
BASE COUNT          456 a         1226 c         1181 g          471 t
ORIGIN      
        1 gaccttgagc agcgagcgcg gcgacagcgc ggtgcggccc tggcagcgca cgccgtcgac
       61 gaacgtgccg ttgcggctgc cgagatcgcg cacgctccag ccggcatcgc tgcgtatcac
      121 caccgcatgc tcgcgcgaca ccgaaaccgc gagcacgacg aggtcgacgt cgtggctgcg
      181 gccgatccgg cactgcgggc cgatcgcgtg ggcgccgccg aacccgtcga ccagccaggc
      241 ctgcgccgcg ctggcgtcga tcgtcgatcg caggtggtcg gggatcagcc catcgcacgg
      301 agcggcctcg cgggcgcagc gacggcacag cacaccgacc tcgggtgcgg caccgcaaca
      361 cggacagccc atggcccgcg atcgaaccac cacgggtcgg cgggtacaag cccccggccg
      421 atcggattgc gcgcgcagcg aacgatccgc ggcgcgctca gcggcggagc tgctcgggca
      481 ggctggtcgc cccgagggtg gtcccgacgg tgaccgggct cgccaggtag tagccgcggc
      541 cgggcgcgac cgccagcagg ccgccgacgc cgacctcggt gagcacgccg cgcagccgga
      601 gcacgacctg gcgcacgttc tcctggttgg cgtacttgga ctggaacggc aggtcgttga
      661 ccagctgctt ggtcggcacg cagccgcgga tcgtcgacgg cgacgacgcc tcctcgtggg
      721 cccggatgca cagcgcgcgc agcagctgga actcgagcgg cgcgaggctg cgctccgacc
      781 aggtgtcggc gccgacctcg cgccacatca gcgcgccggc cgaggcgtcg ttgtcggtgg
      841 cgacgatgca cagctccttg tcgccgcgga ccagctggta gcggaccagc ccgccccggg
      901 tgccgtcggt cgtcatcgcc ggccgccgga ccggctcgga gacgatctcg gcgaggaacc
      961 acatcgcgac gtcgccgacc cggagcaccg cgcgcccagg cagcacggcc gcgccctgga
     1021 cacgcacgcc gttgacgaac gtgccgttgc ggctcccgag gtcggtcacc gtccagccgg
     1081 cgtcggcgtg ctggagctcg gcgtgctcgc gtgagaccga gctggcgagg acgatcagct
     1141 cgttgtcatg attgcgcccg atccgggatc gcacggcgat cggatgagcg ccgccgaaac
     1201 cgtcgacaac ccaggcctcg gcgtcggtcg agtcgatccg ggagtggatg tgatcctgga
     1261 tcagaccctc gcaggggctg acctccttcg cgcagctccg gcacaacgca ccgatctccg
     1321 gctctgcacc acaacacagg caacccatat ccagacgatc gaaacatcag tacgccggcc
     1381 gacacaagtg caaacgtcgg aatcgtgaaa cctgcgactg tcgatcacgc acagagctgg
     1441 ccgaccgtgc ggttgacagg ccgcgcatcg tgcgcctgcg cctcagcgat ggcattctgc
     1501 tgcgttcctc ccgcgcttgc cgccgcgtgc cgcgtgccgc gcgccccggc tcgccgccga
     1561 ccgcgcgctg cagtggcgtc agcgcaagcc agcctccgcc acgcgctgcc gccgtgaaac
     1621 ctccggccgt acgacgacgt gctgccgttc ggatgcgtcg gtgatcgcca ccggcccgct
     1681 gccgcgtacg ttgtgtttcc gtgtaccgcc ggacatcacg aggacgtcgc cgccggcgtg
     1741 ggcatccggg tgtcgacccg cgtgaccttg ccggtgcccg cgatcgtaac gccgccgccg
     1801 acctggatca ggtagctcgc gggtagcgac gggacgccct ggagatcgag cgtgccgagc
     1861 acgggatggc ggcgccggcc gcgatgtcga cggtggtccg ggcaggcatc gtccagcgcc
     1921 gctcctgcgt ggggtgcggc tcacgcgtca gtctcgctcc tgtgacgcgg ccgccgcgcg
     1981 ctcgtcacct ccgcagtccc ggccggcggt cgcgacgcag tgcagcgcga gcgcgtcgag
     2041 cccgccgacg cggtcgagcg cgctgatctg gggcagcggc gcgagctggt gctgcttgag
     2101 ctcgtcgtcg agcgcgcgcg cgtccttgcc gacgatcgta tcgaccgtgg ccgccacgtc
     2161 gccgagctcc cggcgcagcg cctcgatcgc atcgagctgg tacttcgcgg gccggccctc
     2221 ccagccgttg agcgcgccgt acagcgagtc gaggtgctcg cggatccgct cctcgccggt
     2281 gatcgcgccg ccctccttgg tggcgacgat cagcttgcgc gcggcctcga gccggtcgcc
     2341 cagcgcgtgc agcttgccgg ccagcgggtc ggcgtcgggc aggcccttga gccggtcgcg
     2401 caccgcctcg cgcgccatgt cgatccgggt ggtgagcttg gacatgtcgc cgaacacgcc
     2461 gtgcgccttc atcaccgcgt cgaactgcgc cttgcggtcg accgcgttgt acggcgcgcg
     2521 gcgatcgagc ccgatcgcga gcttggtctc gacgacctcg gcgccgcggg tcagccgcac
     2581 cgtgtacgtg cccggcggga cccgcgggcc ctgcgacgac gcgaacgcga tctgcgccgc
     2641 gcgcggcacc cggggcggct tgacccgcat cgcccagctg atgcggttga tcccgcgccg
     2701 cttgctcggc gcgatcgtgt cgaccagctt gccggcggcg tcgaggatct cgagcttgat
     2761 cggaccgtag acgtggcgcg atcggaggta gtacgtgatc gcggcgccgc ccggcgcgct
     2821 gtcgccgctg aacgtggcgt cgccctcggc ccagccgccg ttggcctgca tccgctgctg
     2881 gacgtcgcgg atcggcagga acgcggtcga cttctgcagc gcatcgtcgc cgagcgcgcg
     2941 cagcggcgcg atgtcgtcga cgatccagat cccgcggccg tgggtcgcga tcaccaggtc
     3001 gccgtcgcgc ggatggacct ggacctcgcg gaccgcgacg ccgtcgggga actcgccgcc
     3061 gcggaactgc gcccaccgcg cgccgccgtc gagcgagatc cacaggccga gctcggtgcc
     3121 gacgaacagc aggtccttct tgatcgtgtc ctccttgatc gtgtgggcgt agccgcgcac
     3181 gccctgatcc ggtccgacga tccgggtcca ggtcttgccg tagtcggtgg tccggaacac
     3241 ccacggcgtc atgtcgccga acgtgtggcg gtcgaacgcc gcgtaggcga ccgccgggtc
     3301 gaaccggctg gcctcgaccc agctgaccca agac
//
LOCUS       DASVOR010001742         3397 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_19087,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001742 DASVOR010000000
VERSION     DASVOR010001742.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19087"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="VF516_32430"
     CDS             <1..200
                     /locus_tag="VF516_32430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112498.1"
                     /translation="SKGVLPFVGRTASSAGVGGSNLDAKNSKASGCHSVACRDRPPHD
                     HRIYRPFRQVDMDRALLSCRI"
     gene            370..1335
                     /locus_tag="VF516_32435"
     CDS             370..1335
                     /locus_tag="VF516_32435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112499.1"
                     /translation="MTRTPFDQFGKEMVSTALESHGLIQTEREVPTDPRRIDVWFSPR
                     ERQAALPDHLGLLGQILRRPCTVEFHHRTPNCESLTSCVIKHGDFLHLLSLDEKRAPL
                     PRGRRPRIPTLWVISAGCPKAGIKGLWLRPSEHWPSGIYDAAPLLWTQLVVVSELPVE
                     RSTLLLRLLGAKAVLRRAMAELKALPEDAPERVLALPILLRLRLEIPTNPAEQTSDDQ
                     EFLMQTQDIVETWRREAVQEGIEKGVKQGLEAGVKQGLEAGVKQGLACSLIDLYEARF
                     GAMPDDLRAIIEDTHDEATLRGWVKLAGTRSADEVAAAIRASRPA"
     gene            1406..2548
                     /locus_tag="VF516_32440"
     CDS             1406..2548
                     /locus_tag="VF516_32440"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX8112500.1"
                     /translation="MRWASAISDHGSGVAALDEAMASVRARLDRLDLAIVFASPSRAR
                     EIAPRLASLPAATVIGCSGAGIIGDAREIEDRPAVSVTAAELPGVTCTAFRVEPDGLP
                     ADAGAWRARIGVEDPAGLLVLVEPFTNAQALVDGLDAAFPHARKFGGLVSGSRMPGTD
                     WLFAQGQAFRTGAVGVAFTGDVVIDTIVAQGCRPIGAPMVVTRGEANLISELDGKPPL
                     DVLQALHDSLDARDRALFRHSLFVGIEMTDSLVHHEGELLVRNILGLDPEANVLAVAA
                     RLRPFQVIRFLLRDANAATEDLVRLLERHRAAGRGACDGALLFSCLGRGAHLFGEPDH
                     DSRLFRAHLGDVAVGGFFCNGEIGQVGGTTFLHGYTSSFALFRGRT"
     gene            2648..>3397
                     /locus_tag="VF516_32445"
     CDS             2648..>3397
                     /locus_tag="VF516_32445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112501.1"
                     /translation="MMDRQAFTCSLLVASGLAACAAGPPKSAALATTPAAREAPPATY
                     QAPVGPAPAASATGYADVNTDSSLISNGSVTASSQELLSTASAFRREVYRLGGRVFAE
                     QVHFRAEGDDDGKQASAASYRVKLLPRLLPDLLDWLGKHATITAQDVQSIIAMESEGD
                     ASIVRADVSARIAEIAGQLADPALDPAYRAALEAERAKLTGAEIADPKAMADNTRRVA
                     VLDVRLEAPAPRDPYARGQLLGHARGSLLGLG"
BASE COUNT          495 a         1166 c         1210 g          526 t
ORIGIN      
        1 gctcgaaggg cgtgttgccg ttcgtcggtc gcaccgccag ttcggctggc gtgggcggca
       61 gcaacctcga tgcgaagaat agcaaggcaa gtggctgtca cagcgttgcc tgccgtgacc
      121 gtccgccgca tgatcatcga atttaccgac cctttcggca ggttgatatg gaccgagcat
      181 tgctgtcatg caggatatga tgctcattac tcgtcccgga tggtgtcgct gactcgttgg
      241 ctgacagcgt gatcgcatcc caacagagca tcgtgctcac attcagcacc acgctcatgc
      301 ggatctctga cggctgcatt cgcaagtcat tttcttgatc tcgcgcatcc cagtggccat
      361 tgtcgcgcag tgacacgcac cccctttgac cagttcggca aggagatggt cagcaccgcg
      421 ctggagagcc acggtctcat ccagaccgag cgcgaagtcc cgacggaccc tcgcaggatc
      481 gacgtctggt tctcgcccag agaacgccag gccgcgctcc cggaccatct cggtctgctc
      541 gggcagatct tgcgccgccc ctgcacggtg gagttccacc acaggacgcc gaactgcgag
      601 tccctcacta gctgtgtgat caagcacggg gatttcctgc acctcctgtc gctggatgag
      661 aagcgcgccc cgctcccacg cgggaggcgg ccgagaatcc cgacactgtg ggtgatctcg
      721 gcagggtgtc ccaaggccgg catcaagggc ctgtggctcc gcccatcgga gcattggccg
      781 tccgggatct atgatgctgc gcctctgctg tggacgcagc tggtcgtcgt cagcgagctg
      841 ccggtcgagc gcagcacgct gctgctgcgc ctgcttggcg ccaaagctgt gctacggagg
      901 gcgatggccg agctcaaggc gttaccggag gatgcgccgg agcgcgtcct ggcgctcccc
      961 atcctgctaa gattgcggct ggagatcccg accaaccccg cggaacagac aagcgacgac
     1021 caggagttcc tcatgcagac ccaagacatc gtcgagacct ggcgccggga ggcggtccag
     1081 gaaggcatcg aaaagggtgt caagcagggc ctcgaagcag gcgtcaagca gggcctcgaa
     1141 gcgggcgtca agcagggcct cgcatgctca ctgatcgatc tgtacgaggc tcgcttcggc
     1201 gcgatgcccg acgacctccg cgccatcatc gaggacacgc acgacgaggc gacgctgcgc
     1261 ggctgggtca agctcgccgg cacgcgtagc gcagacgaag tggcagcggc gatccgcgca
     1321 tcccgcccag cgtgatcgcc gcgccgcggc gtggctcgcg gtgaatgtca ttgcacgcgg
     1381 cggcggatgg tggcatgccg gtggcatgcg atgggcctcg gcgatctccg accatggttc
     1441 cggcgtcgcg gcgctcgacg aggcaatggc gtccgtgcgc gcccggctcg accggctcga
     1501 tctcgcgatc gtgttcgcgt cgccgtcgcg cgcgagggag atcgcgcctc ggctcgcgag
     1561 cctgcccgcc gcgacggtga tcggctgctc cggggccgga atcatcggcg acgcgcgcga
     1621 gatcgaggat cgccctgcgg tatcggtgac cgcggcggag ctgcccgggg tgacgtgcac
     1681 ggcgttccgg gtcgagccgg atgggctgcc ggcggacgcc ggcgcgtggc gcgcgcggat
     1741 cggcgtcgag gacccggccg ggttgctggt gctggtcgag ccgttcacca acgcgcaggc
     1801 gctggtcgac gggctcgatg ccgcgttccc gcacgcgcgg aagttcggcg ggctggtcag
     1861 cggcagtcgc atgccgggga ccgactggct gttcgcacag ggccaggcgt tccgcaccgg
     1921 ggcggtcggg gtcgcgttca ccggcgacgt cgtgatcgac acgatcgtcg cgcagggctg
     1981 ccggccgatc ggcgcgccga tggtggtgac ccgcggcgag gcgaacctga tctcggagct
     2041 cgacggcaag ccgccgctgg acgtgctgca ggcgctgcac gactcgctcg acgcccgcga
     2101 ccgcgcgctg ttccgccact cgctgttcgt cgggatcgag atgacggaca gcctggtgca
     2161 tcacgagggc gagctgctgg tgcgcaacat cctcgggctc gatccggagg cgaacgtgct
     2221 cgcggtggcc gcccggctgc ggccgttcca ggtgatccgg ttcctcttgc gcgacgcgaa
     2281 cgcggcgacc gaggatctgg tgcggctgct cgagcggcac cgcgcagccg gccgcggcgc
     2341 gtgcgacggt gcgctgctgt tctcgtgcct gggccgcggg gcgcacctgt tcggtgagcc
     2401 ggatcacgac agccggctgt tccgcgcgca cctcggcgac gtcgcggtcg gcgggttctt
     2461 ctgcaacggc gagatcgggc aggtcggcgg cacgacgttc ctgcacggct acaccagctc
     2521 gttcgcgctg ttccgcggcc ggacgtgagc cgcacgcgcg ccgcggggcg ttgatccgcc
     2581 cccggcaacg ccggtcgcgc cgtttgctta caatgaaagt cgccggggcg gccggcgttg
     2641 tccggccatg atggatcggc aagcattcac gtgttcgctc ctcgtcgcca gcggcctcgc
     2701 tgcatgtgcg gcgggcccac ccaagagcgc cgcgctggcc accacgccgg ccgcccgcga
     2761 ggcaccgccg gcgacgtacc aggcgccggt cggccccgcg cccgcggcgt cggcgaccgg
     2821 ctacgccgac gtcaacaccg acagctcgct gatcagcaat ggctcggtca ccgcgtcgtc
     2881 gcaggagctg ctttcgaccg ccagcgcgtt ccggcgcgag gtctaccggc tcggcggccg
     2941 ggtgttcgcg gagcaggtgc acttccgcgc ggaaggcgac gacgacggca agcaggcgtc
     3001 cgccgcgagc taccgggtca agctgctgcc ccgcctgctg cccgatctgc tcgactggct
     3061 cggcaagcac gcgacgatca cggcgcagga cgtgcagtcg atcatcgcga tggagagcga
     3121 gggcgacgcg tcgatcgtcc gcgccgacgt gagcgcgcgg atcgccgaga tcgccggcca
     3181 gctcgccgat ccggcgctcg atccggcgta ccgggcggcg ctcgaggccg agcgcgcgaa
     3241 gctgaccggc gcggagatcg ccgatccgaa ggcgatggcc gacaacaccc ggcgcgtcgc
     3301 ggtgctcgac gtccggctcg aggcgccggc cccgcgcgat ccgtacgccc gcggccagct
     3361 gctcggtcac gcgcgcggct cgctgctcgg gctcggc
//
LOCUS       DASVOR010001743         2690 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_106507,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001743 DASVOR010000000
VERSION     DASVOR010001743.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106507"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VF516_32450"
     CDS             complement(<1..1079)
                     /locus_tag="VF516_32450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEX8112502.1"
                     /translation="MSRSLLGPGRNEPKREPDSIVVEGAREHNLVVDRLELPKHRLVV
                     ITGPSGSGKSSLAFDTLYAEGQRRYVESLSAYARQFLGQMEKPKYERIRGLSPTIAIQ
                     QKSASSNPRSTVGTITEIYDYLRVLYARAGEQRCHQCGGPVGARTAGEVVDELAALPD
                     RTSVTLLAPKAENRKGEFRELFAELRKAGFVRVRIDGMIVRLEDVDALEKQKKHSIEL
                     VIDRLTINPKDRGRLTDSVETALREGKGKLMVEVGDEKVPRMYSEANACPKCGIGFPE
                     LSPQSFSFNSPLGMCVECNGLGDRLAADPDLVIPDPSRSLRDGAVAVWGEAIAKDSGW
                     TTNIVKALAKAFKIDLDRPWNKLSD"
     gene            complement(1219..1704)
                     /locus_tag="VF516_32455"
     CDS             complement(1219..1704)
                     /locus_tag="VF516_32455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112503.1"
                     /translation="MAALVLAVAAPAYAEGPPTPFDQGHVSVNLAVTEQSAFGANRVG
                     LGAGAGYFVLDGLELGVFGLHMFGSGPSLDEVSPSIRYTAQPLVGRWPVIPYVGAFYN
                     HWFVGDGLEDVDAVGARAGVVHLRGRLIVGLGIAYEHTVSTCTSSCDTVYPDVMFGFT
                     F"
     gene            complement(1864..>2690)
                     /locus_tag="VF516_32460"
     CDS             complement(1864..>2690)
                     /locus_tag="VF516_32460"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HEX8112504.1"
                     /translation="EDRPQHHRAEQRSEHHDRGDRKGRRDKRSEPTADTREFWETWAD
                     EKSARVSEARSAEPAPRGKPEPHRAAAVEPAVEPAVEAAEPEAVEPEAAEPEAREAAP
                     DATDRRSKERGRGRDRDQDRGQDRGRDRGRDRDESRGRTEAKGHDRERHGKPERDRHD
                     KDRHGKAERHEHPDKPDRSTRGKRDTAAPPAPAADGAQARLFVSLGKKHGVSADDLRE
                     LLAGPLGGDKSRIGSVSLRDSHAHVRVPEEYVDAIIAGVHGTQHNDQDVTVERARA"
BASE COUNT          337 a          945 c          992 g          416 t
ORIGIN      
        1 ttgtcggaca gcttgttcca cggccggtcg aggtcgatct tgaacgcctt ggccagcgcc
       61 ttgacgatgt tggtggtcca gcccgagtcc ttggcgatcg cctcgcccca caccgcgacc
      121 gcgccgtcgc gcagcgaccg gctcgggtcc gggatcacga gatcggggtc ggcggcgagc
      181 cggtcgccga ggccgttgca ctcgacgcac atcccgagcg gcgagttgaa cgagaagctc
      241 tgcggcgaca gctcggggaa gccgatgccg cacttggggc acgcgttggc ctcggagtac
      301 atccgcggca ccttctcgtc gccgacctcg accatcagct tgcccttgcc ctcgcgcagc
      361 gcggtctcga ccgagtcggt cagccggccc cggtccttgg ggttgatcgt gagccggtcg
      421 atcaccagct cgatcgagtg cttcttctgc ttctcgagcg cgtcgacgtc ctcgagccgg
      481 acgatcatgc cgtcgatccg gacccggacg aacccggcct tgcgcagctc ggcgaacagc
      541 tcgcggaact cgcccttgcg gttctcggcc ttgggggcga gcagcgtgac ggaggtcctg
      601 tcgggcagcg cggcgagctc gtcgacgacc tcgccggccg tgcgcgcacc gaccggaccg
      661 ccgcactggt ggcagcgctg ctcgccggcg cgcgcgtaca gcacgcgcag gtagtcgtag
      721 atctcggtga tcgtgccgac cgtcgagcgc gggttcgacg acgcgctctt ctgctggatc
      781 gcgatggtcg gcgacagccc gcggatccgc tcgtacttgg gcttctccat ctggccgagg
      841 aactggcgcg cataggccga cagcgactcg acgtagcggc gctggccctc ggcgtacagc
      901 gtgtcgaacg cgagcgacga cttgcccgag cccgacggcc cggtgatcac gaccagccgg
      961 tgcttgggga gctcgagccg gtcgacgacc aggttgtgct cgcgagcacc ctcgacgacg
     1021 atgctgtctg gctctcgctt gggttcgttg cgtccgggcc cgagcaagct gcgggacata
     1081 ggcacgacac cgtacggacc acatgttcag gtgtcaagcc ggccacggtg ctggctgcgc
     1141 gcggggcgag ggcggcgctg tcaaacctga caacccgtgg tcagcgatcg ccggccgctc
     1201 aggcgatcgc cgagccggtc agaacgtgaa gccgaacatg acgtcggggt agaccgtgtc
     1261 gcagctcgag gtgcaggtcg acaccgtgtg ctcgtaggcg atgccgaggc cgacgatcaa
     1321 ccggccgcgc aggtgcacga ccccggcccg ggcaccgacg gcgtcgacat cctccaggcc
     1381 gtcgccgacg aaccagtggt tgtagaacgc gccgacgtac gggatcaccg gccagcggcc
     1441 gaccagcggc tgggcggtgt accggatcga cggcgagacc tcgtcgaggc tggggccgct
     1501 gccgaacatg tggagcccga acacgccgag ctcgaggccg tcgagcacga agtagccggc
     1561 gccggcgccg agcccgacgc ggttggcgcc gaacgcgctc tgctcggtga ccgccaggtt
     1621 cacgctgacg tggccctggt cgaacggggt cggcggcccc tccgcgtacg cgggagcggc
     1681 cacggccagg acaagcgcag ccacccaccc ggatcggacc atggcgcgag tatgcctcga
     1741 accggggcga cgcagccccc gggtgcgcga accatggggc catacgcggg cggtctgccg
     1801 gtcgcccagg gccgggagga cagaccgcag accgcagacc ggtagaccgc cggctcgggc
     1861 gcgtcaggcg cgggcgcgct cgaccgtgac gtcctggtcg ttgtgctggg tgccgtggac
     1921 gccggcgatg atcgcgtcga cgtactcctc ggggacccgg acgtgggcgt gcgagtcgcg
     1981 caggctgacc gacccgatcc ggctcttgtc gccgccgagc gggccggcga gcagctcgcg
     2041 gaggtcgtcg gcggacaccc cgtgcttctt gcccaggctc acgaacagcc gggcctgggc
     2101 gccgtctgcc gccggggcgg gcggggccgc ggtgtcgcgc ttgccgcgcg tggagcggtc
     2161 gggcttgtca gggtgctcat gacgctccgc cttgccgtgg cggtccttgt cgtggcgatc
     2221 gcgctccggc ttgccgtggc gctcccggtc atgacccttg gcctcggttc gaccgcgact
     2281 ctcatcccgg tcccggccgc ggtcccggcc tcgatcctgg cctcggtcct ggtctcggtc
     2341 tcggcctcgg ccgcgctcct tgctgcggcg gtcggtggcg tccggagcgg cctcgcgcgc
     2401 ttcgggctcg gcggcctcgg gctcgacggc ctcgggttcg gcggcctcga cggctggctc
     2461 gacggcgggt tcgaccgcgg cagcccggtg gggctccggc ttgccgcggg gggcaggctc
     2521 cgcggaccgc gcctcgctga cccgggcgga cttctcgtcg gcccaggtct cccagaactc
     2581 acgggtgtcg gcggtgggct cggaccgctt gtcgcgccgg cccttgcgat cgcctcgatc
     2641 gtggtgctcg ctgcgctgct cggcgcggtg gtgctggggg cggtcctcgg
//
LOCUS       DASVOR010001744         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_426387,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001744 DASVOR010000000
VERSION     DASVOR010001744.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426387"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="VF516_32465"
     CDS             complement(<1..1168)
                     /locus_tag="VF516_32465"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8112505.1"
                     /translation="MSDDAAPRTTLWRHADFVKLWSAQSISLLGTQITTLALPLTAIL
                     LLHATPVQVGLLASMQYLPFLLVTLPAGAIVDRRRRLPVLIATDLGRAVLLAAIPLAV
                     WLGHLELWLLYPVAFGVGVLSVFFDVAHQSYLPSIVDGEQLVEGNTKLQLSYSGAQLA
                     GPGLGGVLVQLLTAPIAIAVDVTSYLGSAVLLGWIRRREPAPPADAGGADLAGIARDI
                     RGGLHFVWRHPLIRPIALATGTANFFYLFGMTGAVLALYAVRELGLSPALLGIVLASG
                     NVGAILGSLAADRVLARFRFGRVMLVGSVASAFAIALMALATPGTAVVMLPAAVMLGE
                     LGVTIYDISQISLRQAATPSALQGRMNATVRFINWGPIPIGAFIGGVIGEALGLR"
     gene            complement(1158..>2268)
                     /locus_tag="VF516_32470"
     CDS             complement(1158..>2268)
                     /locus_tag="VF516_32470"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase domain-containing protein"
                     /protein_id="HEX8112506.1"
                     /translation="GVFDLAGAVALVAARGRLLQDLPDGAMVAVPRSERHVQELLRPG
                     LAIAAVNGPEQCVVSGPRTEIDRFLEALRDREIDARMLRISAAAHSVLVDRALPRFRA
                     TVAALGRRAPAIPMVSDHTGTWLTGEQATDPGYWAAHLRGTVRFSSALDTLLGEDAPW
                     IVLEVGPGRTLATLTRQHPAHQARHLVLTSLPHATADEPELPHLLDAAGRLWLTGQEL
                     AWAHLHDGEAPRRVPLPTYPFQRMRFRVDPDPAIPVTIEPVPETGGDDERDTGHVAPS
                     TATERAVAGAFEAILGVPRAGAGDSFLDLGGDSLIAAQLTAWIRRTYRLPVAIRAIFK
                     HPTVASLARLIDAGLAAGEPPAATAPTAQEIHRVR"
BASE COUNT          330 a          777 c          910 g          251 t
ORIGIN      
        1 tgcgcagccc gagcgcctct ccgatcacgc cgccgatgaa cgcgccgatc gggatcggtc
       61 cccagttgat gaaccggacg gtggcgttca tgcggccctg cagcgccgac ggggtcgccg
      121 cctggcgcag gctgatctgg ctgatgtcgt agatcgtgac gccgagctcg ccgagcatca
      181 ccgcggcggg cagcatcacc acggcggtgc ccggcgtcgc cagggccatc agcgcgatcg
      241 cgaacgccga ggcgaccgag ccgacgagca tgacccggcc aaaccggaac cgggcgagca
      301 cgcggtcggc cgcgagcgag ccgaggatcg cgccgacgtt gccggaggcg agcacgatcc
      361 cgagcagcgc cgggctgagc ccgagctcgc ggacggcgta gagcgcgagc accgcgccgg
      421 tcatcccgaa caggtagaag aagttggccg tgccggtggc cagcgcgatc gggcggatca
      481 gcgggtggcg ccagacgaag tgcagcccgc cgcggatgtc gcgggcgatc ccggcgaggt
      541 cggcgccgcc ggcgtcggcc ggtggcgcgg gctcgcgccg ccggatccag ccgagcagca
      601 ccgccgagcc caggtagctg gtgacgtcga cggcgatcgc gatcggagcg gtgagcagct
      661 ggacgagcac gccgcccagg cccgggccgg cgagctgcgc gccggagtac gacagctgga
      721 gcttggtgtt gccctcgacg agctgctcgc cgtccacgat cgacggcagg tacgactggt
      781 gcgcgacgtc gaagaacacc gacagcacgc cgaccccgaa cgccaccggg tagagcagcc
      841 agagctccag gtggccgagc cagaccgcga gcgggatcgc ggcgagcagc acggcgcggc
      901 cgaggtcggt cgcgatcagc accggcagcc ggcgcctgcg gtcgacgatc gccccggccg
      961 gcagcgtgac cagcaggaac gggaggtact gcatgctggc gagcagcccg acctgcacgg
     1021 gcgtggcgtg gagcaggagg atcgcggtga gcggcagcgc cagcgtcgtg atctgggtgc
     1081 cgagcaggct gatcgactgc gcggaccaca gcttgacgaa gtcggcgtgc cgccagagcg
     1141 tggtgcgggg agcggcatca tcggacacgg tggatctcct gggcggtggg ggcggtggcc
     1201 gcgggtggtt cgccggcggc caggccggcg tcgatcaggc gagcgaggct cgcgacggtc
     1261 gggtgcttga agatcgcgcg gatcgcgacg gggagccggt aggtgcgccg gatccaggcc
     1321 gtcagctggg cggcgatcag cgagtcgcct ccgagatcga ggaagctgtc accggcgccg
     1381 gcgcggggca cgccgaggat ggcctcgaac gcgccggcga ccgcacgctc ggtggcggtg
     1441 ctcggggcga cgtggccggt gtcgcgctcg tcgtcgccgc cggtctcggg cacgggctcg
     1501 atggtcaccg ggatcgcggg atcgggatcg acgcggaatc gcatgcgctg gaacggatag
     1561 gtcggcagcg ggacgcggcg cggcgcctcg ccgtcgtgca ggtgcgccca ggcgagctcc
     1621 tggccggtga gccacagccg gccggccgcg tcgagcaggt gcggcagctc gggctcgtcg
     1681 gcggtggcgt gcggcaggct ggtgagcacc aggtggcggg cctggtgggc cgggtgctgc
     1741 cgggtcaggg tggccagcgt tcgcccgggg ccgacctcga gcacgatcca cggcgcgtcc
     1801 tcgccgagca gggtatcgag cgcgctcgag aaccgcaccg tgccgcgcag gtgagccgcc
     1861 cagtacccgg gatcggtggc ctgctcgccg gtgagccacg tgccggtgtg gtcggagacc
     1921 atcgggatcg cgggcgcgcg ccgcccgagc gccgccaccg tggcgcggaa ccgcggcagc
     1981 gcccggtcga cgagcaccga atgcgccgcc gccgagatcc gcagcatccg ggcgtcgatc
     2041 tcgcggtcgc gcagcgcctc gaggaagcgg tcgatctcgg tccgcggacc cgacaccacg
     2101 cactgctcgg ggccgttgac cgcggcgatc gcgaggccgg ggcggagcag ctcctgcacg
     2161 tgccgctcgg accgcgggac cgcgaccatc gcgccgtcgg gcagatcctg cagcagccgg
     2221 ccgcgcgccg cgaccagcgc gaccgcgccg gccaggtcga acacgccg
//
LOCUS       DASVOR010001745         7234 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_193550,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001745 DASVOR010000000
VERSION     DASVOR010001745.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193550"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..6891
                     /locus_tag="VF516_32475"
     CDS             31..6891
                     /locus_tag="VF516_32475"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX8112507.1"
                     /translation="MAAGSYYLILATDGYGNRVPESDDTNNTIAVPVTVGQADLVVSS
                     LTGVPAQSSSQQLLPVSWTVKNQATTGAASGSFVPCCAQTDHGSYQYNNIWFDRFFLS
                     TDATLSTEDTFIAEYARPDNFTLAAGASYTASASIHVPAVAAGSYYLILVTDGYGNRV
                     PESNDTNNTIAVPITVGQADLVVSSLTGVPAQAAAQQLVPVSWTVTNQATAGAASGSF
                     VPCCTQTDHGSYQYNNIWFDRFFLSTDATLSAGDTFIGEYARPDNFTLAAGASYTASA
                     NVQLPAVAQGNYYIILVTDAYGNRVPESDETNNTIAIPITVGQADLVVSSLTGVPAQA
                     SSQQLLSVSWTVANQATFGAASGSLVPCCTQTDHGSYQYNHIWFDRFFLSTDATLGTD
                     DVFIGEYARPDNFTLAAGASYTASASIHVPTVAAGSYYLILVTDGYGNRVPESNETNN
                     TVAVPITVGQADLVVSSLTGVPGQAAAQQLLPVSWTVINQSTAGAASGSLVPCCTQTD
                     HGSYQYNNLWFDRFFLSTDATLSTDDTFIGEYARPDNFTLAAGASYTASATIQLPAVA
                     QGNYYVILVTDGYGNRVPESNETNNTIAVPVTVGQADLVVSSLTGVPAQASSQQLLPV
                     SWTVTNQASLGAASGSLVPCCTQTDHGSYQYNHIWFDRFFLSTDATLSADDTFIGEYA
                     RPDNFTLAAGGSYTATASIHVPPVAAGSYYLILITDGYGNRVPESNETNNTFSVPITV
                     GQADLVVSSLTGVPAQASSQQLLPVSWTVTNQATAGAARGSLVPCCAQTDHGSYQYNN
                     LWFDRFFLSTDATLSADDTFIGEYARPDNFTLAAGGSYTASATVQLPAAAPGSYYVIL
                     VTDGYGNRVPESNETNNTIAVPVTVGQADLVVSSLTGVPAQASSQQLLPVSWTVTNQA
                     TTGAASGSLVPCCTQTDHGSYQYNHIWFDRFFLSTDATLSADDVFIGEYARPDGFTLA
                     AGGSYTATASIQVPAVASGSYYVIVITDGYGNRVQESNENNNIVAVPMTVGQADLVVS
                     SLTGVPAQASSQQLLPVSWTVTNQAATGAASGSLVPCCTQTDHGSYQYNHIWFDRFFL
                     SADATLSADDTFIGEYARPDNFTLAAGAGYTASASIHVPTVTPGSYYLILVTDGYGNR
                     VPESDETNNTVSVPITVGQADLAVAAVTGMPSGVSSQQLVPIAWTVTNQAATGAASGS
                     LIPCCTQTDHGSYQYNNLWFDRFFISTDATLSADDTFIGEYARPDGFGLAAGASYTAT
                     ATLQMPDLPAGSYYLIFATDAYGDRVPESNESNNTFAVPFNTLGRPDLVAVALAGAPA
                     QASSQQPLQLTWTVKNQATGGAASGSFVPCCAQTDHGSYQYNQRWFDRLFLSTDATLS
                     ADDTFLGEYARPDGFALAGGASYSGTATIQWPKVPAGSYYLIFTTDGYGNRVRETDET
                     NNTVVVPITSVTNADLRVANLSAPTGALGNKQLASISWTVTNGGTGTAWHSWQDKIYL
                     SQNPVFDSTAIYVDFVSHTTDLPAGASYNVTKDITLPNLTTGSYYFLVVTDAADAIAE
                     ANETNNGAASAAPITVRPTALVQPAVTLALPSGAARITRSPVFSPDGTRLAIADGNRA
                     DLWSLDTLASTGTFAGHTNAVTSVRFSPLGDQVLSASLDGTLKTWDPADFSQKAVLHQ
                     SNYYNPAVFSSDGTRILAGSNSNTGVATLWDAPRGVVLGSLVAGYNVTAVALSPNGAV
                     AVTGGDGGGATLWNMATFTQIAQLHGHTDTVNAVAFSPDGSEFLTASSDGSIRFWGSA
                     TGASLGTIVQGRHVVDARYSGDGQYIVSCDDYAPGHTTQNSQAYLFTRGGLLIAVFQP
                     QGWVDGVSATLGGVRPGFTGVALSPDSTTLATTYKTNSTDPVAGVFLWKTGLPAIPIV
                     PAVPVALASSHPFTIQPGGKYLFEFTVAAGQPGLVIHLSGTPNGATYPSSAGADPVAV
                     QMYSSQRGMPSPTSHLDAADVRSSTLSGNLLASPIVGGTYRVLVTAPSLSGGSINAEI
                     SANYSGFFLSSVDGNKVGNAGRATFRVRGIQLPPSAIGLVSPSSGTLAPVAVSYRDPT
                     RVFATFDLTGAPVGSYDLQATSGSTTLTLAGAVTVTTGTGPALSFSLQAPPAIRKNRA
                     YDYAVTYVNSGDSDAVAPFVSVTPAEDGALFYPDGTHRPGSGIWLAGADGWPSAVIPA
                     GASGILRFRASWKTTAGVTLSQLPEDATPFDWMQVLSQLPPANSPQGWTQAQAVMGST
                     YGQVLVKMRGIAAIGTGPVDFTQVALSSLLVSLQGNRF"
     gene            6909..>7234
                     /locus_tag="VF516_32480"
     CDS             6909..>7234
                     /locus_tag="VF516_32480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112508.1"
                     /translation="MRRVAWILTLVVATVVARRALADGYGCDSAAAGLTCTPVGACCD
                     AHDAGFNKYGCTAGSWSCLNPFALTPESCINQGGSYDMRSGYCTLSGSVKPTCSGDNP
                     ADATSN"
BASE COUNT         1231 a         2648 c         2230 g         1125 t
ORIGIN      
        1 accgcgagcg cgagcatcca tgtcccgtcg gtggccgccg gctcctatta cctgatcctc
       61 gccaccgacg gctacgggaa tcgcgtgccg gagagcgatg acaccaacaa cacgatcgcg
      121 gtcccggtca ccgtggggca ggcggacctg gtggtctcct cgctcaccgg cgtgccggca
      181 cagtcgtcct cgcagcagct cctcccggtg tcgtggacgg tgaagaacca ggccacgacc
      241 ggcgcggcca gcggctcctt cgtgccctgc tgtgcccaga ccgatcacgg atcgtatcaa
      301 tacaacaata tctggttcga tcgattcttc ctgtcgaccg acgcgacgct cagcaccgag
      361 gacacgttca tcgccgagta cgcccggccc gacaacttca ccctggccgc cggcgccagc
      421 tataccgcga gcgcgagtat ccatgtcccg gcggtggccg ccggctccta ttacctgatc
      481 ctcgtcaccg atggctacgg gaatcgcgtg ccggagagca atgacaccaa caacacgatc
      541 gcggtcccga tcaccgtggg gcaagcggac ctggtggtct cttcgctcac cggcgtcccc
      601 gcgcaggcgg cggcgcagca gctcgtgccg gtgtcgtgga cggtgaccaa tcaggccacg
      661 gccggcgcgg ccagcggctc cttcgtgccc tgctgcaccc agaccgatca cggatcgtat
      721 caatacaaca atatctggtt cgaccggttc ttcctgtcga ccgacgcgac gctcagcgcc
      781 ggcgacacgt tcatcggcga atacgcccgg cccgataact tcaccctggc cgccggcgcc
      841 agctacacgg cgagcgcgaa cgttcagctc cccgccgtgg ctcaaggcaa ctattacatc
      901 atcctggtca ccgacgcgta tggcaaccgc gtgccggaga gcgacgagac caacaacacg
      961 atcgcgatcc cgatcaccgt ggggcaggcg gacctggtgg tgtcctcgct caccggcgta
     1021 ccggcacagg cgtcctcgca acagctcctg tcggtgtcgt ggaccgtggc caatcaggcc
     1081 accttcggcg cggccagcgg ctccctggtt ccgtgctgca cccagaccga tcacggatcg
     1141 tatcaataca accacatctg gttcgaccgg ttcttcctgt cgaccgacgc gacgctcggc
     1201 accgacgacg tcttcatcgg cgagtacgcc cggcccgata acttcaccct ggccgccggc
     1261 gccagctata ccgcgagcgc gagcatccat gtcccgacgg tggccgccgg ctcctattac
     1321 ctgatcctcg tcaccgacgg ctacgggaat cgcgtgccgg agagcaacga gaccaacaac
     1381 acggtcgcgg tcccgatcac cgtggggcaa gcggacctgg tggtctcttc actcaccggc
     1441 gtccccgggc aggcggcggc gcagcagctc ctcccggtgt cgtggacggt gatcaatcag
     1501 tccacggccg gcgcggccag cggctccctg gtcccctgct gcacccagac cgatcacggg
     1561 tcgtatcaat acaacaacct ctggttcgat cgattcttcc tgtcgaccga cgcgacgctc
     1621 agcaccgacg acacgttcat cggcgaatat gcccggcccg acaacttcac cctggccgcc
     1681 ggcgccagct acacggcgag cgcgaccatt cagctccccg ccgtggccca aggcaactat
     1741 tacgtcatcc tggtcaccga cgggtatggc aaccgtgtgc cggagagcaa cgagaccaac
     1801 aacacgatcg cagtccccgt caccgtgggg caggcggacc tggtggtgtc ctcgctcacc
     1861 ggcgtgccgg cacaggcgtc ctcacaacag ctcctgccgg tgtcgtggac ggtgaccaat
     1921 caggcctccc tcggcgcggc cagcggctcc ctggtcccct gctgcaccca gaccgatcac
     1981 gggtcgtatc aatacaacca catctggttc gaccggttct tcctgtcgac cgacgcgacg
     2041 ctcagcgcgg acgacacgtt catcggcgaa tatgcccggc ccgacaactt caccctggcc
     2101 gccggcggca gctataccgc gaccgcgagc atccatgtcc cgccggtggc cgccggctcc
     2161 tattacctga tcctcatcac cgacggctac gggaatcgcg tgccggagag caacgagacc
     2221 aacaacacct tctctgtccc catcaccgtc gggcaagcag acctggtggt ctcatcgctc
     2281 accggcgtgc cggcacaggc gtcctcgcaa cagctcttgc cggtgtcgtg gaccgtgacc
     2341 aatcaggcca cggccggcgc ggcccgcggc tccttggtcc cctgctgcgc ccagaccgat
     2401 cacggatcgt atcaatacaa caacctctgg ttcgatcgct tcttcctgtc gaccgacgcg
     2461 acgctcagcg ccgacgacac gttcatcggc gaatatgccc ggcccgacaa cttcaccctg
     2521 gccgccggcg gcagctacac ggcgagcgcg accgttcagc tccccgccgc ggcccccggc
     2581 agctattacg tcatcctggt caccgacgga tatggcaacc gcgtgccgga gagcaacgag
     2641 accaacaaca cgatcgcggt ccccgtcacc gtgggacagg cggacctggt ggtgtcctcg
     2701 ctcaccggcg tgccggcaca ggcgtcctcg cagcagctcc tcccggtgtc gtggaccgtg
     2761 accaatcagg ccacgaccgg cgcggccagc ggctccctgg tcccctgctg cacccagacc
     2821 gaccacggat cgtatcaata caatcacatc tggttcgacc ggttcttcct gtcgaccgat
     2881 gcgacgctca gcgccgacga cgtcttcatc ggcgaatatg cccggcccga cggtttcacg
     2941 ctcgcggcgg gcggaagcta cacggcgacg gccagcattc aagtcccggc ggtggcctcg
     3001 ggctcctatt acgtcatcgt catcaccgac ggctacggca accgcgtgca agagagcaac
     3061 gagaataaca acatcgtcgc cgttcccatg accgtggggc aggcggacct ggtggtgtcc
     3121 tcgctcaccg gcgtgccggc acaggcgtcc tcgcaacagc tcctgccggt atcgtggacc
     3181 gtgaccaatc aggccgcgac cggcgcggcc agcggctccc tggtcccctg ctgcacccag
     3241 accgatcacg ggtcgtatca atacaaccac atctggttcg accgcttctt cctgtcggcc
     3301 gacgcgacgc tcagcgcgga cgacacgttc atcggcgaat atgcccggcc cgacaacttc
     3361 acgctggccg ccggtgccgg ctacaccgcg agcgcgagca tccacgtccc gaccgtgacg
     3421 ccgggctcgt attacctgat cctcgtcacc gacggctacg ggaatcgcgt gccggagagc
     3481 gatgagacca acaacaccgt ctctgtcccc atcaccgtgg ggcaagcgga cctggcggtc
     3541 gccgcggtga cgggcatgcc gtcgggggtg tcttcgcagc agctggtccc catcgcgtgg
     3601 accgtcacca accaggccgc caccggcgcg gccagcggct ccctgatccc ctgctgcacc
     3661 cagaccgatc acgggtcgta tcaatacaac aacctctggt tcgatcgctt cttcatatcg
     3721 accgacgcga cgctcagcgc cgatgacacc ttcatcggcg aatacgcgcg gccggacgga
     3781 ttcgggctgg ccgcgggggc cagctacacg gcgaccgcga ccctgcagat gccggatctg
     3841 cccgcgggct cctactacct catcttcgcc accgacgcct acggcgaccg ggtgccggag
     3901 agcaacgaga gcaacaacac gttcgcggtg ccgttcaaca cgctcggccg ccccgatctg
     3961 gtggcggtgg ccctggcggg cgctccggcc caggcctcgt cgcagcaacc cctgcagctc
     4021 acctggacgg tgaagaatca ggccacgggg ggcgcggcca gcggctcctt cgtgccgtgc
     4081 tgcgcgcaga ccgaccacgg atcgtatcag tacaaccagc gctggttcga tcgcctgttc
     4141 ctgtcgaccg acgcgacgct cagcgccgac gacaccttcc tcggcgaata cgcgcggccc
     4201 gatgggttcg cgctcgcggg tggggccagc tacagcggga cggcgaccat ccagtggcca
     4261 aaggttccgg cgggctccta ttatctcatc ttcacgaccg acgggtacgg caaccgcgtg
     4321 cgggagaccg acgagaccaa caacaccgtg gtggttccca tcaccagcgt caccaacgcc
     4381 gacctgaggg tcgcgaacct gtcggcgccg accggcgcgc tcggaaacaa gcagctcgcc
     4441 agcatctcgt ggacggtcac caacggggga accgggaccg cctggcactc ctggcaggac
     4501 aagatctacc tgtcccagaa cccggtcttc gactccactg ccatctacgt ggacttcgtg
     4561 tctcacacca ccgacctgcc ggcgggcgcg agctacaacg tcaccaagga catcacgctg
     4621 cccaacctga cgaccgggag ttactacttc ctcgtcgtca ccgacgcggc cgatgcgatc
     4681 gccgaggcca acgaaaccaa taacggggcg gcgtcggccg cgccgatcac ggtgcgtccg
     4741 accgcgctgg tgcagccggc ggtcaccctc gccctgccga gcggcgccgc gcggatcacg
     4801 cgcagcccgg tgttctctcc cgacggaacg cggctggcga tcgccgacgg caatcgcgcc
     4861 gacctgtgga gcctggacac gctggcgtcg accgggacct tcgccggcca caccaacgcg
     4921 gtgacctcgg tgcggttctc gccgctcggc gatcaggtcc tgtcggcgtc gctcgacggc
     4981 accctcaaga cctgggaccc cgccgacttt tctcagaagg cagtcctcca tcaatcgaac
     5041 tactacaatc cggcggtgtt ctcgtccgac ggcacccgca tcctcgccgg cagcaacagc
     5101 aacaccgggg tcgccactct gtgggatgca ccgcgcggcg tggtgctcgg atcgctggtc
     5161 gccgggtaca acgtcaccgc ggtggcgctg tctcccaacg gcgccgtggc ggtcaccggc
     5221 ggcgacggcg gcggcgccac gctgtggaac atggccacct tcacccagat cgcccagctc
     5281 cacggacaca ccgacaccgt gaacgcggtg gcgttctcgc ccgacgggag cgagttcttg
     5341 acggccagca gcgacgggag catccggttc tggggctcgg ccaccggcgc ctcactcggg
     5401 accatcgtcc agggccggca cgtggtcgac gcccgctact ccggtgacgg ccagtacatc
     5461 gtgtcctgcg acgactacgc cccgggccac accacccaga acagccaggc gtatctgttc
     5521 acgcgcggtg ggctcctgat cgccgtgttc cagccccagg gatgggtcga cggcgtgagc
     5581 gcgaccctgg gcggcgtccg tcccgggttc acgggggtgg cgctctcgcc ggacagcacc
     5641 acgctcgcga ccacctacaa gacgaacagc accgatccgg tggcgggcgt cttcctgtgg
     5701 aagaccggat tgccggcgat ccccatcgtg ccggcggtgc cggtggccct ggcgagcagc
     5761 catcccttca ccatccagcc aggagggaaa tacctgttcg agttcacggt cgccgccggg
     5821 cagcccggcc tggtgatcca cctgtcggga actcccaacg gcgcgaccta tccgagcagc
     5881 gcgggcgccg acccggtggc ggtgcagatg tactcgagcc agagaggcat gccgtccccg
     5941 acgagtcacc tcgacgcggc cgacgtccga tcctcgaccc tgagcggcaa cctcctggct
     6001 tcgcccatcg tgggcggaac ctaccgcgtg ctggtcacag cgccgtcgct cagcggcgga
     6061 tcgatcaacg cggagatcag cgccaactac agcggcttct tcctgtcctc ggtcgacggc
     6121 aacaaggtgg ggaacgccgg gcgggcgacc ttccgcgtgc gcggcatcca gctccccccg
     6181 agcgcaatcg ggctcgtctc tccgtcttcc gggacgctgg ctccggtcgc cgtgagctac
     6241 cgggacccga cccgggtgtt cgcaaccttc gacctgaccg gcgcgcccgt gggctcgtac
     6301 gacctgcagg cgacctccgg ctcgacgacg ctgacgctcg ccggagcggt gaccgtcacg
     6361 accgggaccg gccccgcgct gagcttctcc ctgcaggcgc cgcccgcgat ccggaagaat
     6421 cgggcctacg actatgcggt gacctacgtg aactccggag attccgacgc ggtcgcgccg
     6481 ttcgtctcgg tgaccccggc ggaagatggc gcgctgttct accccgatgg gacccatcga
     6541 cccggctcgg gaatctggct cgccggcgcc gacggatggc cgagcgcggt catcccggcc
     6601 ggcgcgagcg ggatcctccg gttccgcgct tcgtggaaaa cgacagcggg tgtaacgctg
     6661 agtcagctgc cggaagacgc cactccgttc gactggatgc aggtgctctc gcagctgccg
     6721 ccggccaact caccgcaggg gtggacccag gcgcaggcgg tgatgggcag cacctacggg
     6781 caggtcctcg tcaagatgcg aggcatcgcg gcgatcggga ccggaccggt ggatttcacc
     6841 caggtcgcgc tgtcttcgct gctggtgtcg ctacaaggga accgattctg atgtccggag
     6901 gagtcaacat gcgcagagtc gcctggatcc tgacgctggt ggtcgcgacg gtggtcgcgc
     6961 ggcgggccct cgccgacggt tacggctgcg actccgccgc ggcgggactg acctgtaccc
     7021 cggtcggagc gtgttgcgac gcgcacgacg ccggcttcaa caagtacggc tgcaccgccg
     7081 gcagctggtc ctgcctcaac ccgttcgccc tgacgccgga gagctgcatc aatcagggag
     7141 gcagctacga catgaggagc ggctactgca cgctctccgg gagcgtgaag ccgacctgca
     7201 gcggggacaa cccggccgac gcgaccagca actg
//
LOCUS       DASVOR010001746         1602 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_456127,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001746 DASVOR010000000
VERSION     DASVOR010001746.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456127"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="VF516_32485"
     CDS             <1..543
                     /locus_tag="VF516_32485"
                     /inference="COORDINATES: protein motif:HMM:NF013962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEX8112509.1"
                     /translation="PIEARVHPALIPASWLLADVGGAKNALYVQSYALGPSLYYGAGA
                     GMLPTAMAVVSDMIELGRNIVGRVAGAPRPTRPRPAQPRPVMALGDIRTRYYLRFGVH
                     DKPGVLGQLMTILGAHHISIAQVVQDGRAAGNEPVWVVVVTHEATERDVRAALAEIDT
                     LDVVRQPARLIRIAGEVTSP"
     gene            540..953
                     /locus_tag="VF516_32490"
     CDS             540..953
                     /locus_tag="VF516_32490"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEX8112510.1"
                     /translation="MTGLHDGTLRHEVRVIFGDTDQMGVVYYANYLRYFEGARAAYWR
                     ALGRSHKDLEAWGVALPVVEAHCHYRRPAHYEDVLGVDVRVSELRGASLRFAYEIHRG
                     GERIADGTTRHAVLGKDGRLRALPPALRDAIPRIG"
     gene            1086..>1602
                     /locus_tag="VF516_32495"
     CDS             1086..>1602
                     /locus_tag="VF516_32495"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class II"
                     /protein_id="HEX8112511.1"
                     /translation="MQDRNLALEVVRITEAAALASARLMGRGDRKLADHVAVEAMRLA
                     FDAIDIRGTVVIGEGERDEAPMLFIGERVGAGWRHDSGSSPRVDIAVDPLEGTNLCAT
                     GAPDAISVIAIADDGQFLNAPDTYMQKIAVGPEARGAIDLRESATWNLQRIARAKGKK
                     VEDCTAVILDRD"
BASE COUNT          229 a          566 c          599 g          208 t
ORIGIN      
        1 ccgatcgagg cgcgggtcca ccccgcgctg atcccggcga gctggctgct cgccgacgtc
       61 ggcggcgcga agaacgcgct gtacgtccag agctacgcgc tcggcccgtc gctgtactac
      121 ggcgcgggag ccggcatgct gccgaccgcg atggcggtgg tctcggacat gatcgagctc
      181 ggccgcaaca tcgtcggccg cgtcgccggc gcgccgcggc cgacccgccc gcgcccggcg
      241 cagccccggc cggtgatggc gctcggcgac atccggaccc gctactattt gcggttcggc
      301 gtccacgaca agcccggcgt gctcggccag ctgatgacca tcctcggcgc gcaccacatc
      361 tcgatcgcgc aggtcgtgca ggacggccgg gccgcgggca acgagccggt gtgggtcgtg
      421 gtggtcaccc acgaggccac cgagcgcgac gtgcgcgccg cgctcgccga gatcgacacg
      481 ctcgacgtcg tccgccagcc ggcgcggctg atccggatcg ccggcgaggt gacatcgccg
      541 tgacgggtct gcacgacggg accctgcgcc acgaggtccg cgtgatcttc ggcgacaccg
      601 accagatggg ggtcgtctac tacgcgaact atctgcgcta cttcgagggc gcccgcgcgg
      661 cgtactggcg ggcgctcggc cggagccaca aggacctgga ggcgtggggc gtcgcgctgc
      721 cggtggtcga ggcgcactgc cactaccgca ggccggcgca ctacgaggac gtgctcggcg
      781 tcgacgtccg ggtcagcgag ctgcgcggcg catcgctccg gttcgcctac gagatccacc
      841 gcggcggcga gcggatcgcc gacgggacca cccgtcacgc ggtgctcggc aaggacggcc
      901 ggctgcgtgc gctgccgccc gcgctgcgcg acgcgatccc gcggatcggc tgaccggcgc
      961 gcggaatcgc ccggtgccag cgcgggatcg cggggtgcgc ggcgggcgcg ggtgagcggg
     1021 tgagatcgac gcgagtcgcg ggccgtaatt gatcgggccg cggcagcagc ggtacacatt
     1081 gtctcatgca agaccgcaac ctcgcgctcg aagtggtccg catcaccgaa gcggcggcgc
     1141 tggcgtcggc ccgcctgatg gggcgcggcg atcgcaagct cgccgatcac gtcgcggtcg
     1201 aggcgatgcg gctcgcgttc gacgcgatcg acatccgcgg caccgtggtg atcggcgaag
     1261 gcgagcgcga cgaggcgccg atgctgttca tcggcgagcg cgtcggcgcc ggctggcgcc
     1321 atgacagcgg cagctcaccg cgcgtggata tcgcggtcga tccgctggag ggcaccaacc
     1381 tgtgcgcgac cggcgccccg gacgcgatca gcgtgatcgc gatcgctgat gacggtcagt
     1441 tcctcaacgc gccggacacc tacatgcaga agatcgcggt cggccccgag gcgcgcggcg
     1501 cgatcgacct gcgcgagtcg gcgacctgga acctccagcg gatcgcccgg gccaagggca
     1561 agaaggtcga ggactgcacg gcggtgatcc tcgaccgcga tc
//
LOCUS       DASVOR010001747         2999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_163896,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001747 DASVOR010000000
VERSION     DASVOR010001747.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163896"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VF516_32500"
     CDS             <1..871
                     /locus_tag="VF516_32500"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112512.1"
                     /translation="QRPDLPASFVVPALGADRVLVGAFYLDGGLVDRDTYVVARDLAA
                     RVALAVQRAQAVSELLDARRLAALGQFAAAIAHDIRTPLTSISMNVQILRRKLRLPDD
                     DREHLDIALEELARLDRSVAEILDFAKPVKLTSEPIDVGELIETCSRSLLPVLSERGV
                     SVRIEPARPGRDGERDALTVHGDPQRLRQVLTNLVDNAANASRPGAEVTLRAAPAGDR
                     CVAIEVEDHGRGIGPDDLPRIFEPFFTTRPDGTGLGLAIVHKVIRAHGGDIKVRSTLG
                     AGSTFTITLPQPA"
     gene            1000..2133
                     /locus_tag="VF516_32505"
     CDS             1000..2133
                     /locus_tag="VF516_32505"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8112513.1"
                     /translation="MWNLARKLLFHDRLRFAVAIAGVSVSVMLVLVQVGLYFGFMDTA
                     SSLIDASHADLWVGKKSNESFEFATPFDDRAFYRVASVAGVAHAERVVMNFAQCKLPD
                     GGDLGVQIVGIDTTPGHRPLLAPWNVVAGDPRRLAEPGAIIVDRTEVPKLKFDRVGHT
                     TEIAGVRAEVVALTRGIRSFTTSPIVFADLRTARSYLSQLGGEPVTYVLVEVAPGARV
                     ADVQARIDALPHLAAYTTAEMSARTRSYWSTRTGVGAGFFTTAVLGVIVGFVVVGQIL
                     YSGTLQYLREYGTLKAMGARNGAVVRVILSQAMISAALGFAVGAPLAIAMRAAMKSAN
                     LSVALTPPLYAATAAITAAMCAFAALLSIVKVLRLDPASVFKG"
     gene            2164..2952
                     /locus_tag="VF516_32510"
     CDS             2164..2952
                     /locus_tag="VF516_32510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8112514.1"
                     /translation="MIALAVNELVSHLGALAPAPPPPAPVARLVGVAKTYGRGENAVA
                     ALAGASLDVHPGEVLLIEGPSGSGKTTLISILGLLLTPTAGEVWLEGKDVAGLGEREL
                     PALRARNFGFVFQGFNLFPALTAAENVAIAIRMKNPRARNADAEARRLLERVGLGPRM
                     HHLPADLSGGQKQRVAIARALGGDPPILIGDEPTAALDTRTALSVMELLRELATSQRR
                     AVVVVTHDPRLERFADRVVRVEDGQIQSITAAGPAGLESGRSMP"
BASE COUNT          383 a         1171 c         1013 g          432 t
ORIGIN      
        1 ccagcggccg gacctgccgg cgagcttcgt ggtgcccgcg ctcggcgccg atcgcgtgct
       61 ggtcggcgcg ttctacctcg acggcggcct ggtcgatcgc gacacctacg tcgtggcgcg
      121 cgatctggcg gcccgggtcg cgctcgcggt ccagcgcgcc caggcggtgt ccgagctgct
      181 cgacgcgcgc cggctcgccg cgctcggcca gttcgccgcg gcgatcgcgc acgacatccg
      241 cacgccgctg accagcatct cgatgaacgt ccagatcctg cgccgcaagc tgcggctgcc
      301 cgacgacgac cgcgagcacc tcgacatcgc gctcgaggag ctcgcccggc tcgatcgctc
      361 ggtcgccgag atcctcgact tcgccaagcc ggtcaagctg acgtccgagc cgatcgacgt
      421 cggcgagctg atcgagacct gcagccgcag cctcttgccg gtgctgtccg agcgcggcgt
      481 gtcggtgcgg atcgagccgg cgcggccggg gcgtgacggc gagcgcgacg cgctgaccgt
      541 gcacggcgat ccgcagcgcc tgcgccaggt gctgaccaac ctggtcgaca acgcggccaa
      601 cgcatcgcgg cccggcgccg aggtcacgct gcgcgcggca ccggccggtg atcgctgcgt
      661 cgcgatcgag gtcgaggatc acggccgcgg gatcggcccc gacgacctgc cgcggatctt
      721 cgagccgttc ttcacgaccc gccccgacgg caccggcctg ggcctcgcga tcgtccacaa
      781 ggtgatccgc gcccacggcg gcgacatcaa ggtccgctcg acgctcggcg ccggctcgac
      841 gttcacgatc acgttgccgc agccggcctg agccccgacg accgccgacg gtaacgtcgt
      901 ttccagcggg gtaacgctcc gttaccgtgg cccgccgggt aacgccgcga aatcgcgcgc
      961 cgcgcggccg gcatgcgagc tgctctgacc gacgggagca tgtggaacct ggctcgcaag
     1021 ctgctctttc acgatcgcct gcggttcgcc gtcgcgatcg ccggtgtgtc ggtctcggtg
     1081 atgctcgtgc tggtccaggt cggcctgtac ttcggcttca tggacaccgc gtcgtcgctg
     1141 atcgacgcca gccacgccga cctgtgggtc ggcaagaaga gcaacgagtc gttcgagttc
     1201 gcgaccccgt tcgacgatcg cgcgttctac cgggtcgcct cggtcgccgg cgtcgcccac
     1261 gccgagcgcg tggtgatgaa cttcgcgcag tgcaagctgc ccgacggcgg cgacctcggc
     1321 gtccagatcg tcgggatcga caccacgccc ggccaccggc cgctgctcgc gccgtggaac
     1381 gtggtcgccg gcgacccccg ccggctcgcc gagccgggcg cgatcatcgt cgaccgcacc
     1441 gaggtcccca agctcaagtt cgaccgggtc ggccacacca ccgagatcgc cggggtgcgc
     1501 gccgaggtgg tcgcgctgac ccgcgggatc cggtcgttca cgacctcgcc gatcgtgttc
     1561 gcggacctgc gcaccgcgcg cagctacctg tcgcagctcg gcggcgaacc ggtgacctac
     1621 gtgctggtcg aggtcgcgcc cggcgcgcgc gtcgccgacg tccaggcgcg gatcgacgcc
     1681 ctgccccacc tcgccgcgta caccaccgcc gagatgtcgg cgcgcacccg cagctactgg
     1741 tcgacccgca ccggcgtcgg cgccgggttc ttcaccaccg ccgtgctcgg cgtgatcgtc
     1801 gggttcgtcg tcgtcggcca gatcctgtac agcggcacgc tgcagtacct ccgcgagtac
     1861 ggcacgctca aggcgatggg cgcccgcaac ggcgcggtcg tccgcgtgat cctgtcgcag
     1921 gcgatgatct ccgcggccct gggcttcgcg gtcggcgcgc cgctcgcgat cgcgatgcgc
     1981 gcggcgatga agtccgccaa cctcagcgtc gcgctgaccc cgccgctgta cgccgcgacc
     2041 gccgcgatca ccgccgcgat gtgcgcgttc gccgcgctgc tctcgatcgt caaggtgctg
     2101 cggctcgacc ccgcgtcggt gttcaagggc tagccgcgcg tcaacccgca tcccgaggcc
     2161 accatgatcg cactcgccgt caacgagctc gtctctcacc ttggggccct ggcccccgct
     2221 ccgcccccgc ccgcgccggt agcccgcctg gtcggcgtcg ccaagaccta cggccgcggc
     2281 gagaacgcgg tcgccgcgct cgccggggcc agcctcgacg tccaccccgg cgaggtcctg
     2341 ctgatcgagg gcccgtcggg ctcgggcaag accacgctga tctcgatcct cggcctcctg
     2401 ctcacgccga ccgccggcga ggtctggctc gagggcaagg acgtcgccgg gctcggcgag
     2461 cgcgagctgc ccgcgctgcg cgcccgcaac ttcggcttcg tgttccaggg cttcaacctg
     2521 tttccggcgc tgaccgcggc cgagaacgtc gcgatcgcga tccgcatgaa gaacccgcgc
     2581 gcccgcaacg ccgacgccga ggcccggcgg ctgctcgagc gggtcggcct gggcccgcgc
     2641 atgcatcacc tgcccgccga tctgtcgggc ggccagaagc agcgcgtcgc gatcgcccgc
     2701 gcgctcggcg gcgatccgcc gatcctgatc ggcgacgagc cgaccgccgc gctcgacacc
     2761 cgcaccgcgc tgtcggtgat ggagctcctg cgcgagctcg ccaccagcca gcgccgcgcc
     2821 gtcgtcgtcg tcacccacga tcctcgcctc gagcggttcg ccgaccgggt ggtccgcgtc
     2881 gaggacggcc agatccagtc gatcaccgcc gccggccccg ccggcctcga gtccggaagg
     2941 agcatgccat gaccaagctg cgtaccctcg ccctcaccgt tgccctcccg ctcgccggc
//
LOCUS       DASVOR010001748         4520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_163898,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001748 DASVOR010000000
VERSION     DASVOR010001748.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163898"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..542
                     /locus_tag="VF516_32515"
     CDS             <1..542
                     /locus_tag="VF516_32515"
                     /inference="COORDINATES: protein motif:HMM:NF013821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="riboflavin kinase"
                     /protein_id="HEX8112515.1"
                     /translation="ARTGTVEDLRAHGSQAGIEVALVPAVTIDGEIAASTKIRGHLRA
                     GELARAERMLGRPWDVDGVVVHGAGRGRAIGVPTANIAPEVELAIAPGIYAVTLSVEG
                     GPAMPAVASLGTNPTFVDDGGLVLEVHVLDWSGDLYDRRVRTTFIARLRDEQKFDSVE
                     ALVSQIRRDIAQARAALAR"
     gene            complement(553..1875)
                     /locus_tag="VF516_32520"
     CDS             complement(553..1875)
                     /locus_tag="VF516_32520"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8112516.1"
                     /translation="MRAPLALAVAPLAFHVALGVALIPAPVAAAPKITILKAAHLFDG
                     KSDQVVSPGVVVVQDGKITAAGAKVQEPAGAEVVDLGNATLIPGLIDAHTHLTNEGSS
                     DWKKDELDRFKKPIPQVAIEATEFARRTLMAGFTTVRDLGSSNLIDIGLRNAINEGMV
                     PGPRMLVSVHAISARGGHCDDTAGYRPDLLKEPGPEEGVADGPDQVRAAVRFNAKHGA
                     DVIKVCASGGVLSLTDKVDSPQLTQAELDALVDESHALGRRTAAHAHGAEAAKRAIRA
                     GIDSIEHGSFLDDQARDLMKQKGTYFIFTPVLCLNDRLKKAGAPAHVVGKAIAATAAA
                     DATFKRALARGVKLGFGSDAAVCPHGSQVAQFADMVRLGMKPLAALRAATSSDAQLLG
                     RDDLGTLEPGKTADVVGVPGDPSRDIAAVEKVFFVMKDGVVYRNDVRR"
     gene            2073..2552
                     /gene="cynS"
                     /locus_tag="VF516_32525"
     CDS             2073..2552
                     /gene="cynS"
                     /locus_tag="VF516_32525"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: protein motif:HMM:NF002773.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HEX8112517.1"
                     /translation="MKREQLTEKILDIKRDNGWTWKHITTEIGGVSPVLVVGALLGQM
                     KLVKPLARKAAALFGLSPSEERMLNEVPHRGSPMPPSDPLLYRLYELVMVNGPAWKAW
                     IEEEFGDGIMSAIDFDVEIERLPSPKGDRVKISMSGKFLPYKYYGNEAGIPDYGLKE"
     gene            3205..4098
                     /locus_tag="VF516_32530"
     CDS             3205..4098
                     /locus_tag="VF516_32530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112518.1"
                     /translation="MTASQLLKILSLSTTLVVSACATDPEATTDELTPIEESTAFHML
                     PLRNVAEGQVANAAPPGAHLSFFGGPVLKNVHNAPLYWNSGVQFQSNLNLFYNDVPNS
                     PLYTMLAQYSAIGHGNGQNGSVDTRATANISDATVQNEVLAQINAGRLPIPNANTYYP
                     VHFPPGMSITSPDGSTSCVQFCAYHGTFRVRNSAGTIINVNYGVIPDQGGGCAGGCGA
                     NPSRVNNLDSVSSHELVEATTDPAVGLATVIGSPLAWYDPNNGEIGDICNGQQGTTTG
                     NGRSYVIQLEFSNAANNCVQQ"
     gene            4130..>4520
                     /locus_tag="VF516_32535"
     CDS             4130..>4520
                     /locus_tag="VF516_32535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112519.1"
                     /translation="MGTPTAPPAGRGGQRPRGIGRRAGAAAAELDDAGPRQEVEPLGD
                     LGERHRRVAAGQQVADVADRALAVDQVERLVGEHLVALLERDRGPRLAEHRREVVVVV
                     DVERAAHGGRALGHPRIPAAAGVRLRRG"
BASE COUNT          704 a         1569 c         1577 g          670 t
ORIGIN      
        1 aggcccggac cggcacggtc gaggatctgc gcgcgcacgg cagccaggcc ggcatcgagg
       61 tcgcgctcgt cccggcggtc accatcgacg gcgagatcgc ggcgtcgacc aagatccgcg
      121 gccacctccg cgccggcgag ctcgcgcgcg ccgagcgcat gctgggccgg ccgtgggacg
      181 tcgacggcgt ggtcgtccac ggcgccgggc gcggccgggc gatcggcgtg ccgaccgcga
      241 acatcgcccc cgaggtcgag ctcgcgatcg cgcccgggat ctacgcggtg acgctcagcg
      301 tcgagggcgg cccggcgatg ccggcggtgg cgagcctggg caccaacccg acgttcgtcg
      361 acgacggcgg cctggtgctc gaggtccacg tgctcgactg gagcggcgac ctctacgacc
      421 ggcgggtccg gaccacgttc atcgcccggc tgcgcgacga gcagaagttc gactcggtcg
      481 aggcgctggt cagccagatc cggcgcgaca tcgcgcaggc gcgcgccgcg ctcgcccggt
      541 gaccgcggcg gatcaccgcc ggacgtcgtt gcggtagacc acgccgtcct tcatcacgaa
      601 gaacaccttc tcgaccgcgg cgatgtcgcg cgacgggtcg ccgggcacgc cgacgacgtc
      661 ggcggtcttg ccgggctcca gcgtgccgag gtcatcgcgc ccgagcagct gggcgtcgct
      721 cgaggtcgcg gcgcgcagcg cggcgagcgg cttcatgccg agccgcacca tgtcggcgaa
      781 ctgcgcgacc tgcgagccgt gcgggcagac cgcggcatcc gagccgaacc cgagcttgac
      841 gccccgggcg agcgcgcgct tgaacgtcgc gtccgcggcc gcggtcgccg cgatcgcctt
      901 gccgaccacg tgggcgggcg cgcccgcctt cttgagccga tcgttcaggc acagcaccgg
      961 cgtgaagatg aagtaggtgc ccttctgctt catcaggtcg cgcgcctggt cgtcgaggaa
     1021 cgagccatgc tcgatcgagt cgatgccggc gcggatcgcg cgcttggcgg cctcggcgcc
     1081 gtgggcgtgc gccgcggtcc gccggccgag cgcgtgggac tcgtcgacca gcgcgtcgag
     1141 ctcggcctgg gtcagctgtg gcgagtcgac cttgtcggtg agcgacagca caccgcccga
     1201 cgcgcagacc ttgatcacgt cggcgccgtg cttggcgttg aaccgcaccg cggcgcgcac
     1261 ctgatcgggg ccgtcggcga cgccctcttc cgggccgggc tccttgagca ggtcgggccg
     1321 gtagccggcg gtgtcgtcgc agtgaccgcc gcgcgccgag atcgcgtgga ccgacaccag
     1381 catgcgcggg ccgggcacca tgccctcgtt gatcgcgttg cgcaggccga tgtcgatcag
     1441 gttcgacgac ccgaggtcgc gcacggtggt gaacccggcc atcagcgtgc ggcgcgcgaa
     1501 ctcggtggcc tcgatcgcga cctgcgggat cggcttcttg aaccggtcga gctcgtcctt
     1561 cttccagtcg ctgctgccct cgttcgtcag gtgggtgtgg gcgtcgatca gcccggggat
     1621 cagcgtggcg ttgccgagat cgacgacctc ggcgccggcc ggctcctgga ccttggcgcc
     1681 cgcggccgtg atcttgccgt cctggaccac gaccaccccg ggcgacacca cctgatccga
     1741 cttgccgtcg aacaggtgag cggccttgag gatggtgatc ttcggcgccg cggcgacggg
     1801 ggcggggatc agggcgacgc cgagggcgac atggaatgcc agcggagcga cagcgagcgc
     1861 gagcggagct ctcatgccgc ggaccataca cgcgcagcag cgacagtgtc tcgccttgcc
     1921 cgacatctcg atgatcggac cgcagtcgcc gcggggagcc gcggacgggg tggctcggtg
     1981 gtccggtctg ttccacggag tggcgctcgc gtcgtgggtt ggacgctgcc ggcgatgcgt
     2041 cgcgtcacgt acgctccgcc gcggagatcg atatgaaacg cgaacagctc accgaaaaga
     2101 ttctcgatat caagcgcgac aacggctgga cctggaagca catcaccacg gagatcggcg
     2161 gcgtatcgcc ggtgctggtg gtcggcgcgc tgctcggcca gatgaagctg gtcaagccgc
     2221 tcgcgcgcaa ggccgcggcg ctgttcgggc tgtcgccgag cgaggagcga atgctcaacg
     2281 aggtgccgca ccgcggcagc ccgatgccac cgagcgatcc gctgctctac cggctgtacg
     2341 agctcgtgat ggtcaacggc ccggcctgga aggcatggat cgaggaggag ttcggcgacg
     2401 gcatcatgtc ggcgatcgac ttcgacgtcg agatcgagcg cctgcccagc cccaagggcg
     2461 accgcgtcaa gatctcgatg tctggcaagt tcctacctta caagtattac gggaacgagg
     2521 ccggcattcc cgactacggg ctgaaggaat gagcaccggc gcgggcgtca gatccccgtg
     2581 atcggcgtga ccggcgtctc gcacggtgcc gtaaaacgcg gcccgagatc ggtcccacgc
     2641 gcaccgcgcg atcaggcaga gtgaacgctc gcgtcgcaca ggactgctcc atatccgtat
     2701 ccgcatccgc tgcgctggaa cctcgcccac cgtcatgacc atgctcgcct ccgtcggagt
     2761 ccgcgccttg aacggcagcg ccgcgccctt cacgtgacgc ggccgactca cgctgagcag
     2821 agcaccgtcg gggaacactg gtccggccac tcggatccgg caacgacccg gcgcccccgg
     2881 gcggcggcgt tgcgccgggc tgtggtgcga tccgcggctg gtcggtgcga aggggcgcgc
     2941 gagtcccggt cggcggtcgt acagccgtga tccagtaggt gttccgcata acgaattttc
     3001 agggcgaatt gcgcaaattc ctgcactcaa tcaaaatttt atatatggca gtctccgaca
     3061 tccacggttg caatcagaca atgggatttg cacccatacg cagtacctct atccggaaat
     3121 aaacattggt tcttcattga cgggtgactt cggctgttgt ttagtgcggc tcccgccgta
     3181 caacccatct ctcagaggaa cctgatgacc gcttcccagc tgctgaagat cctgtccctg
     3241 tcgaccacgc tggtggtctc ggcctgcgcc accgatccgg aggccacgac cgacgagctc
     3301 accccgatcg aggagagcac cgccttccac atgctgccgc tgcgcaacgt cgccgagggc
     3361 caggtcgcca acgccgcgcc gccgggcgcc cacctgtcgt tcttcggcgg gccggtgctc
     3421 aagaatgtcc acaatgcgcc cctgtactgg aactccggcg tgcagttcca gtccaacctg
     3481 aacctgttct acaacgacgt gcccaacagc ccgctgtaca ccatgctggc tcagtacagc
     3541 gcgatcgggc acggcaacgg tcagaacggc tccgtcgaca cccgggcgac cgcgaacatc
     3601 tcggatgcca cggtccagaa cgaggtgctg gcccagatca acgcggggcg gctgccgatc
     3661 ccgaacgcga acacctacta cccggtccac ttcccgccgg gcatgtcgat cacctcaccg
     3721 gacgggagca cgtcttgcgt gcagttctgc gcgtaccacg gcacgttccg ggtccggaac
     3781 agcgccggga cgatcatcaa cgtcaactac ggcgtgatcc ccgatcaggg cggcggctgc
     3841 gcgggcggct gcggcgccaa cccgtcgcgc gtcaacaacc tcgactcggt ctcgtcgcac
     3901 gagctggtcg aggcgaccac cgacccggcg gtcggcctgg cgaccgtgat cggatcgccg
     3961 ctcgcgtggt acgacccgaa caacggcgag atcggtgaca tctgcaacgg ccagcagggc
     4021 acgacgaccg gcaacggccg ctcctacgtg atccagctcg agttctcgaa cgcggccaac
     4081 aactgcgtcc agcagtagcc gcgcagggtt cgaccggagg gagacggggg tggggacccc
     4141 gacggctccg ccggcgggga ggggggggca gcgcccccgt gggatcggtc ggcgcgccgg
     4201 cgcagcggcc gcggaacttg acgacgccgg ccctcgccag gaggtcgagc cactcggcga
     4261 cctcggcgag cggcaccggc gtgtagcggc cggtcagcag gtcgcggatg tcgccgaccg
     4321 agcgcttgcc gtcgaccagg ttgagcgcct cgtaggcgag cacctcgtcg cgcttctcga
     4381 gcgcgaccgg ggcccgcgcc tcgccgagca ccgccgcgag gtggttgtag tagtagacgt
     4441 cgagcgggcc gcgcacggcg gccgtgcgct cgggcacccg cggatcccgg ctgccgccgg
     4501 cgtgcggctg cgacgcggtg
//
LOCUS       DASVOR010001749         2660 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_193606,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001749 DASVOR010000000
VERSION     DASVOR010001749.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193606"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..1976)
                     /locus_tag="VF516_32540"
     CDS             complement(150..1976)
                     /locus_tag="VF516_32540"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX8112520.1"
                     /translation="MTLIGCTTATDATQNANARDHAADALQHLAGAPVALEVGVTGDM
                     RVLAMTPQFSVPGHATDPVQAATGFLATHHDVFQLDAGDASQFVVTRVDSDRAGDLQH
                     VTLQRVFNGIPVFQGAITVHLDSGNGVFRVLGDESYHIAPPTNRMVLDPGGAAAAAAR
                     ALGVTISPTLTEADGLHAVFESPGLLDPMHVDPQIVHVSDGDDRFAFQATVSWLDDQK
                     QQQYQLVLLDAQDGALLGSYSLVDTFQGKVFNVNAQPTANQSTDTRTEQSFDGNPAAS
                     PSGWVTTPGNKTIGNNAVAATDLNGNNTVGTNETQPTAVNNAFDFPFDPTVDASNFKA
                     AAVTNAFFLVNDHHDRTYALGFTETAGNFQTNNFGKGGAQNDPVNVDAQDGSGTNNAN
                     FATPPDGQRPRMQMFLFNLKNGAGGVRQDGDFDPTVIYHENTHGVSNRLVGGGTTRCL
                     GALQSGGMGEGWSDWVAATFLNNPVIGAYVTGNATRGIRTAPMNNSPWTYNDVKNGTL
                     NEVHKVGELWAATLWDVRTALGAATTNQLVISGMKLTPCNPTMLNARDAIISADANIN
                     AGANKCAIWRAFAGRLMGTGASSPSASSTTQIVTSTAVPAGC"
     gene            complement(2236..>2660)
                     /locus_tag="VF516_32545"
     CDS             complement(2236..>2660)
                     /locus_tag="VF516_32545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112521.1"
                     /translation="STGHRLHEPPPPPAIALRAIAQQGNLMTHSIHQGHDHVHGANCG
                     HLLVQHAGHIDYLHDGHLHNIHGDHIDEHTIAVDATNPDQCTPNHRCTGHAAGHVHGP
                     SCGHPGVPHGDHTDYLVEGHLHHAHNGHCDDHGPLRAA"
BASE COUNT          355 a          835 c          996 g          474 t
ORIGIN      
        1 tcgcgccagc tcaacaccct cgagcagcgg gtcgcgcccg agctcgccgc ccagctcgcc
       61 gccacccggc gcgccctcga cgagcgcgac cgcgaggacc agacccggct gcgtcacctg
      121 cgcagccgat cggcccggcc atctcgcggc tagcagccgg ccgggacggc ggtcgacgtg
      181 acgatctgcg tcgtcgagct ggcgctcggc gacgacgcgc cggtgcccat caggcgaccg
      241 gcgaacgcgc gccagatcgc gcacttgttg gcgccggcgt tgatgttggc gtcggcgctg
      301 atgatcgcgt cgcgcgcgtt cagcatcgtc gggttgcacg gcgtgagctt catgccggag
      361 atgaccagct ggttggtcgt cgcggcgccg agcgcggtgc ggacgtccca cagcgtggcg
      421 gcccacagct cgccgacctt gtggacctcg ttgagcgtgc cgttcttgac gtcgttgtag
      481 gtccacggcg agttgttcat cggggccgtg cggatgccgc gggtcgcgtt gccggtcacg
      541 taggccccga tgaccgggtt gttcaggaac gtcgcggcga cccagtcact ccagccctcc
      601 cccatgccgc ccgactgcag ggcgccgagg cagcgggtcg ttccgccgcc gacgaggcgg
      661 ttggacacgc cgtgggtgtt ctcgtggtag atgacggtcg gatcgaagtc gccgtcctgg
      721 cggacgccgc cggcgccgtt cttgaggttg aacaggaaca tctgcatgcg cggccgctgg
      781 ccgtcgggcg gggtcgcgaa gttggcgttg ttggtgcccg agccgtcctg ggcatcgacg
      841 ttgaccgggt cgttctgtgc gccacccttg ccgaagttgt tggtctggaa gttgccggcg
      901 gtctcggtga agccgagcgc gtaggtccgg tcgtggtggt cgttgaccag gaagaacgcg
      961 ttggtcaccg cggcggcctt gaagttggac gcgtcgaccg tcgggtcgaa cgggaagtcg
     1021 aacgcgttgt tgaccgcggt cggctgggtc tcgttggtgc cgaccgtgtt gttgccattg
     1081 aggtcggtcg cggcgaccgc gttgttgccg atcgtcttgt tgcccggcgt ggtgacccag
     1141 ccgctgggcg acgccgccgg gttgccgtcg aacgactgct cggtgcgggt gtcggtcgac
     1201 tggttcgcgg tcggctgggc gttgacgttg aacaccttgc cctggaacgt gtcgaccagc
     1261 gagtagctgc cgagcagcgc gccgtcctgg gcgtcgagca gcacgagctg gtactgctgc
     1321 tgcttctgat catcgagcca cgacaccgtc gcctggaacg cgaaccgatc gtcgccgtcg
     1381 ctgacgtgga cgatctgcgg atcgacgtgc atcgggtcga gcaagccggg tgactcgaac
     1441 accgcatgca gcccgtcggc ctcggtcagc gtgggcgaga tcgtcacgcc cagcgcgcgg
     1501 gccgcggcgg ccgccgcccc gcctggatcg aggaccatcc ggttggtcgg cggggcaatg
     1561 tggtacgact cgtcgccgag cacgcggaac acaccgttgc cggaatccag gtggaccgtg
     1621 atggccccct ggaacaccgg gatcccgttg aacacccgct gcagcgtgac gtgctgcagg
     1681 tcgccggcgc gatcgctgtc gacgcgcgtg accacgaact ggctcgcgtc gccggcgtcg
     1741 agctggaaca cgtcgtgatg cgtggcgagg aacccggtcg ccgcctgcac cgggtcggtg
     1801 gcatggccgg gcaccgagaa ctgcggcgtc atcgcgagga cgcgcatgtc gccggtgacg
     1861 ccgacctcga gcgcgacggg cgcgccggcc aggtgctgca gcgcgtcggc cgcgtggtcg
     1921 cgagcattgg cattctgggt tgcgtcggtg gctgtcgtac aaccgatcag agtcaatgca
     1981 gataacgaga atactggtgc gaggtgcgag gtggttggca tgtgcggatt gttcacaacc
     2041 gagtgctgat gtccctgccg caaaacacgg ctcggttgcc gcagcgcgct cgcatcgcgc
     2101 ggtcacgttg ccctacgtgt ccgcagcttc gccgacatca tccggagaca aactgcccat
     2161 attcgcgacc ggtcacccgg tcggcgctcg gcggcgccga ccgactgcga tggagactcc
     2221 gtgaacggcg ctgcgtcagg ccgcccgcag cggaccgtgg tcgtcgcagt ggccgttgtg
     2281 ggcgtggtgc agatggccct cgaccaggta gtcggtgtga tcgccgtgcg gcacgcccgg
     2341 gtgtccgcag ctggggccgt ggacgtggcc ggcggcgtgg ccggtgcagc gatggttcgg
     2401 cgtgcactgg tccgggttgg tcgcgtcgac ggcgatcgtg tgctcgtcga tgtgatcgcc
     2461 gtggatgttg tgcaggtgcc catcgtggag gtagtcgatg tggccggcgt gctggaccaa
     2521 gaggtggccg cagttggcac cgtgcacgtg gtcgtggcct tggtggatcg agtgcgtcat
     2581 gagattcccc tgctgggcga tcgcccgaag tgcgatggct ggtggaggcg gtggttcgtg
     2641 gaggcgatga cccgtggagt
//
LOCUS       DASVOR010001750         3954 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_397230,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001750 DASVOR010000000
VERSION     DASVOR010001750.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3954
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397230"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VF516_32550"
     CDS             complement(<1..306)
                     /locus_tag="VF516_32550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112522.1"
                     /translation="MLAQRAGPDGNGELSRITPEIGRARFQRYLERAEFSRYEDLVAP
                     IVRVSADGTVGWVIAQVRVEGTYREDDGVRTPLDTTWAWVELYEHAAGGWRMTGIAST
                     "
     gene            575..823
                     /locus_tag="VF516_32555"
     CDS             575..823
                     /locus_tag="VF516_32555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112523.1"
                     /translation="MAVPQQLLQQLLALDEAVRLEIAHTLLKSVDEAVDDDMSDEERA
                     RLEAALERSLAQADAGQTVPADEVFRALRAKRAARAAR"
     gene            820..1134
                     /locus_tag="VF516_32560"
     CDS             820..1134
                     /locus_tag="VF516_32560"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEX8112524.1"
                     /translation="MTTATVEFSDDARRMLDDADERWVAEHGYLAANPLVEEAEHATE
                     LLRHNPELGVRFRRAQGRRPEIRRLLLQTGWHLYYSVDASRSYVLILAVWYTSRGGHP
                     PL"
     gene            complement(1621..2676)
                     /locus_tag="VF516_32565"
     CDS             complement(1621..2676)
                     /locus_tag="VF516_32565"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEX8112525.1"
                     /translation="MTSQDDTPKGELILYQTEDGRTRIDCRFEGETLWLTQALMAELF
                     QTTLQNINLHLQHIYEEGELDPGATIKSYLMVRQEGTRQVRRPVQHYSLDAILAVGFR
                     VRSHRGTQFRKWANARLSEYLVKGFAMDDERLKNPPGEGHTDYFDELLERIRDIRASE
                     RRFYQKVLDIYATSIDYQLDTDLSQQFFATVQNKMHWAAHGHTAAEIVHERADGEQPF
                     MGMQTTPPGGVVRKADATIAKNYLTEPELQILHRIVNLYIEFAELQALERKPMTMRDW
                     VDKLDEFLKASGRKLLDHAGTISAEVAKAKAEREYERYQALQDAKPRMIDAAFEATVK
                     QLKKPARTRAKKRRGES"
     gene            complement(2673..3005)
                     /locus_tag="VF516_32570"
     CDS             complement(2673..3005)
                     /locus_tag="VF516_32570"
                     /inference="COORDINATES: protein motif:HMM:NF036995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 N-terminal domain-containing protein"
                     /protein_id="HEX8112526.1"
                     /translation="MPTKSSARDDDGLYERVAQILEQSRGRVARSVNTAMVQAYWLVG
                     REIVEVEQAGEQRAGYGQNVLARLAARLRAREAWATRQLEQQIGSLLFERLANHRDWP
                     RAGKGAAA"
     gene            3296..3868
                     /locus_tag="VF516_32575"
     CDS             3296..3868
                     /locus_tag="VF516_32575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112527.1"
                     /translation="MSNDEDEDAGWTTLEFLRTKIAVRDVARMTLQGAPKIELLAKYP
                     ELRDQDIDLATRVASAPLDNALYEEVDEGRFRRHPDHTPRVQISEFDTWVGGHNIPET
                     FEYGKGWWDYVELVRRFAARLDPEDVRVIGHDVVDTPPPRELLPMPAIALSIPGVTFA
                     LRFDFGSWSWKHDAVVNHLPVRNGGRPGVV"
BASE COUNT          633 a         1334 c         1305 g          682 t
ORIGIN      
        1 cgtcgaggcg atgccggtca tccgccagcc gccggcggcg tgctcgtaga gctccaccca
       61 cgcccacgtg gtgtcgagcg gcgtgcgaac gccgtcgtcc tcgcggtacg tgccctcgac
      121 gcggacctgc gcgatcaccc accccaccgt gccgtcggcc gacacgcgca cgatcggcgc
      181 gacgaggtcc tcgtagcgcg agaactcggc gcgctcgagg tagcgctgga accgcgctcg
      241 cccgatctcc ggtgtgatgc ggctgagctc gccgttgccg tcgggaccgg ccctctgtgc
      301 cagcacgtca cctgaaccgc atgcctgcgt cgtggtgtgc ggctcggatc atccgcgatt
      361 ggcgatgtag atccccatgg ggggccgaga catcccagat cgagcgagac gcgtacgtga
      421 cgatggcgct cggctcgtgc caccgagccc caaggccgcg aagttccagc ggaaactccg
      481 atcggtcaag tagcgctgcc acgccggtgt cacgtgaccc gacctgcgaa gacctcgttc
      541 gaccttcgcc accggcttcg ggtaggcttg aaccatggcc gtaccgcaac agctcctcca
      601 gcagctcctc gcgctcgacg aggcggtgcg gctggagatc gcccacacgc tcctgaagag
      661 cgtcgacgag gcggtggatg acgacatgag cgacgaagaa cgggctcggc tcgaggccgc
      721 gctcgaacgt tcgctggcac aggcggatgc cggccagacc gtgcccgcgg acgaggtgtt
      781 ccgggcactg cgtgccaagc gggcagcccg agctgctcgt tgacaaccgc gacggtcgag
      841 ttctcggacg acgccaggcg gatgctcgac gacgccgacg agcgatgggt cgccgagcat
      901 ggttacctcg ccgcgaaccc gctggttgaa gaggccgagc acgcaaccga gctgctccgc
      961 cacaaccccg agctgggcgt gcggttccgt cgagctcagg gccgccggcc tgagatccgg
     1021 cgcctgctgc ttcaaacagg ctggcacctc tactacagcg tcgatgccag ccgatcctac
     1081 gtactgatcc tcgccgtctg gtataccagc cgaggcgggc acccaccgct ctaacgaatg
     1141 agcgacgccg cgtgcaactc gacgccgacc gactcccacg agcgattggt gcagccggcg
     1201 caggatccgg cctgtcacgc cggccgagca agccggtggc gaacatggca cccgtggtac
     1261 ggacgccgtg ttgcaggcgg ccgtgcgtcc cgttgaggga tccgcggctt acgaggatca
     1321 ggctcaggat cggggggtga gggcagaagg actcgaacct tcaaccagcg gattaagagt
     1381 ccgggcggtc gaccttcgga tggtcaccca agaccgcgac ttcacagggt tatgccacgt
     1441 acgggcctcg cgcgagagcc ccgtgtgcca cccgtgtgcc acggcgatcc caacggtggc
     1501 tccgatcccg agcacaattg acccgatggt cggcaatcag cgacgagctg cagcgcaggg
     1561 cgctcgtccg cagctccagc gtgagcgatg cagcgcgctt gacgcgatcg atgaggccgt
     1621 tcaggactcc ccgcgccgct tcttcgcgcg tgttcgagca ggcttcttga gctgcttcac
     1681 cgtcgcctcg aatgcggcgt cgatcatgcg cggcttcgca tcctgcagtg cctggtagcg
     1741 ctcatactca cgctcggcct tcgccttcgc gacctccgcc gagatggttc ctgcgtggtc
     1801 gagtagcttg cggcccgatg ccttgaggaa ctcatcgagc ttgtcgaccc aatcgcgcat
     1861 cgtcatcggc ttccgctcga gcgcctgcag ctcggcgaac tcgatgtaga ggttcacgat
     1921 gcggtggagg atctgcagct cgggctcggt caggtagttc ttcgcgatcg tcgcgtcggc
     1981 cttgcggacc acgcctcccg gcggcgtcgt ctgcatgccc atgaagggct gctcgccgtc
     2041 agcgcgctcg tggacgatct cggcggccgt gtgcccgtgc gccgcccagt gcatcttgtt
     2101 ctgcaccgtc gcgaagaact gctgcgagag gtcagtgtcg agctggtagt cgatgctcgt
     2161 cgcataaatg tcgaggacct tctggtagaa gcgacgctcg gacgcgcgga tgtcacgaat
     2221 ccgctcgagc agctcgtcga agtagtcggt gtgtccttcc ccgggcgggt tcttcagccg
     2281 ctcgtcgtcc atcgcgaagc ccttgaccag gtactcggac agccgcgcgt tggcccactt
     2341 gcggaactgc gtgccgcgat ggctgcggac gcggaacccg acggcgagga tcgcgtccag
     2401 cgagtagtgc tgcacgggac gccggacctg ccgcgtcccc tcctgtcgaa ccatcaagta
     2461 agacttgata gttgccccgg ggtcgagctc cccctcctcg tagatgtgtt gcaggtggag
     2521 gttgatgttc tggagcgtcg tctggaacag ctcggccatg agggcctgcg tgagccagag
     2581 cgtctcgccc tcgaagcggc agtcgatgcg cgtgcgcccg tcctcggtct ggtagaggat
     2641 cagctcgccc ttgggcgtgt cgtcctgcga ggtcacgctg cggctccttt ccctgcgcgc
     2701 ggccagtcgc ggtgattcgc gagccgctcg aacaagagcg agccgatctg ttgctcgagc
     2761 tgacgggtcg cccaggcttc gcgcgccctc aggcgcgctg cgagccgcgc gaggacgttc
     2821 tggccgtagc ctgcgcgctg ctcgccggcc tgctcgacct cgacgatctc gcggccgacc
     2881 agccagtacg cctggaccat cgcggtgttg acgctacggg ccacccgccc tctcgattgc
     2941 tcgaggatct gcgcgacgcg ctcgtagagc ccgtcatcat cacgcgctga gctcttcgtc
     3001 ggcatcgtcc tcgtcatcat cgctatcatg gtccgcgagg tcgaggttcc atgcgcatcg
     3061 tcgtcaagaa tccttcgccg ggccagcctt ggcctccttg tggagccgat gctgctgcct
     3121 cctggcgctt gcgaaccttc agcatcctcg ccgctcctcc tcccgctcga gccacgggac
     3181 ggagcaaccg atccccgccg aggcccggtg gtgacagcgg agcaagccac cgcgtcaagc
     3241 atcgtcgtgc gccagccagc cgggtaccct tgatcagcgc acccaaggag gcttcgtgag
     3301 caacgacgag gatgaagatg ctggatggac aacgctcgaa tttctccgaa ccaagatcgc
     3361 ggtgcgcgac gtcgcgcgaa tgacgttgca aggcgcgccg aagatcgagc tgctcgcgaa
     3421 gtaccccgag ctccgagatc aggacatcga cctggcgacg cgggtcgcga gcgccccgct
     3481 ggataacgct ctctacgagg aggtcgacga gggtcggttc aggcgccacc ccgaccacac
     3541 ccctcgggtt cagatctcgg agttcgacac gtgggtgggt gggcacaaca tccccgaaac
     3601 cttcgagtac gggaagggct ggtgggacta cgtggagctg gttcgccgat tcgccgcacg
     3661 cctggacccc gaggacgtgc gcgtgatcgg gcacgacgtc gtcgatacgc caccgccacg
     3721 cgagctgctc cccatgccgg ccatcgcgct ctccattcca ggcgtcacgt tcgcgctccg
     3781 tttcgatttc ggtagctggt cgtggaagca cgatgcggtt gtcaatcacc taccagtccg
     3841 gaatggcgga cgcccagggg tggtgtgagg tgtaaggcaa gcaggggcgc gcgacgtgct
     3901 ggccgtgtga gggtcggagc tgcggagcgc gaccgaagcg cccctgccgt cgat
//
LOCUS       DASVOR010001751         3722 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_252161,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001751 DASVOR010000000
VERSION     DASVOR010001751.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3722
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252161"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VF516_32580"
     CDS             <1..802
                     /locus_tag="VF516_32580"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HEX8112528.1"
                     /translation="EQYILAELLRQRLAGRGIAAELVESLGSNVVFDALRRGEVDVYV
                     DYSGTLWTTQLGRDRGAPHWQILAEVEGHLADEGVRSLGPLGFQNAYALAVRRDTATR
                     FGLRSVGDLAPHAAGMALGADYEFLSRREWTAVRTTYGLRFARTASFDPALLYEAVAR
                     GEVDVISAFSSDGRIAALDLVVLDDPAAALPSYDAMILLGPRVAGDPEVVCALAPLRD
                     ALSVELMRRANLRVDRDHETPRDAAAWLLGHAAITEACAPPAAAAPPR"
     gene            1174..1515
                     /locus_tag="VF516_32585"
     CDS             1174..1515
                     /locus_tag="VF516_32585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112529.1"
                     /translation="MRSLEILLVADSELVAQALSATLRRAGHRVTASTTIAGTVETLM
                     RRGPHVDIIMTSTRFDSALGDDVVQYSRKFAANARFIVLREVAGHVEDIERALIQQAV
                     PERLGTARGWN"
     gene            complement(1583..1957)
                     /locus_tag="VF516_32590"
     CDS             complement(1583..1957)
                     /locus_tag="VF516_32590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112530.1"
                     /translation="MDRLTVLIVDDDPDIRMLLCELFARAGFRVEVATDGAAAILFLE
                     HNEPPSAIILDLLMPGIIGSSVLAYLASRPVLASVPVAIVSSSPHLAPEGYPLFKKPL
                     QFAPLLEFVRTACVARRPSPPC"
     gene            complement(2047..>3722)
                     /locus_tag="VF516_32595"
     CDS             complement(2047..>3722)
                     /locus_tag="VF516_32595"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8112531.1"
                     /translation="IDPVIRVLQGRDPALALRIAGRELLPEGPRFAVLAEGPGAGDDD
                     PLAWAFGGLGIADRAKYLASLYIDDLADVIRDGIAPEFALSRYAEQLAASAPSFDPLR
                     RALEGEPTLIDELLDELAGELCARHRPDVVGLTVPFPGNVYGAFRIARAIRRVSPATR
                     IVLGGGYVNTELRELAEPAVFDYVDYVTLDDGEAPLLALLRHLADPAAPLYRTFVRER
                     DAVRLVSDDRLRDVALRDAGTPSYAGLALDRYLSLFEMLNPMHRLWADGRWNKLTIAH
                     GCYWKQCAFCDVTLDYIARYDRAPADLLVDRIEAMIAETGQSGFHFVDEAAPPAALRA
                     LAERLIARGVTITWWGNVRFEAAFTPELAQLLARSGCVAVSGGLEVASDRLLALMNKG
                     ITVEQVARVTRAFSDAGVMVHAYLMYGFPTETAQDTIDALERVRQLFAADCLQSAFWH
                     RFTATVHSPIGRAPALYGIRLRAGAPATFARNDVAFDDPTGCDHDLFAAGLRKAVYNF
                     MHGVGLDADPRSWFELPARRGRRGAGPKIPRASVPPDLIRRALADLPGS"
BASE COUNT          574 a         1304 c         1346 g          498 t
ORIGIN      
        1 cgagcagtac atactcgccg agctgctgcg ccagcggctg gcaggccgcg gcatcgcggc
       61 cgagctggtc gagagcctgg gctcgaacgt ggtgttcgat gcgctgcgcc gcggcgaggt
      121 cgatgtctac gtcgactact cgggcacgct gtggacgacc cagctcgggc gcgaccgcgg
      181 tgcgccgcac tggcagatcc tcgccgaggt cgagggccac ctcgccgacg agggcgtgcg
      241 cagcctcggc cccctgggct tccagaacgc gtacgcgctg gcggtccggc gcgacaccgc
      301 gacccggttc ggcctgcgca gcgtgggcga cctcgcgccc cacgccgccg gcatggcgct
      361 cggtgccgat tacgagttcc tgagtcgccg cgagtggacg gcggtgcgga cgacgtacgg
      421 gcttcggttc gcgcgcaccg cgagcttcga tccggcgctg ctctacgagg cggtcgcgcg
      481 cggcgaggtc gatgtgatct cggcgttctc cagcgacggc cggatcgcgg cgctcgacct
      541 ggtcgtgctc gacgacccgg ccgcggcgct gccgagctac gatgccatga tcctgctcgg
      601 gccgcgcgtc gccggcgatc ccgaggtcgt gtgcgcgctg gcgccgctgc gcgacgcgct
      661 gtcggtggag ctgatgcggc gcgcgaacct gcgggtcgac cgcgatcacg agacgccgcg
      721 cgacgccgcc gcgtggctgc tcggccatgc cgcgatcacc gaggcgtgcg ctccaccggc
      781 cgcggcggca ccgccgcgct gatcgcactc ccggcgcggt ccggccactg cggcgggccg
      841 ctacaagaag ctcggaggca agatcgggca cgccccggac gccgccggac cgcccgccga
      901 gctggcggag caccttggtc gaggcgctgc tcgccggtgg cgtcgacccc ggcatgccgg
      961 ccacgcacgg agccgcattc gcgataccga cctgcggcga ctcctgtctc gccacagaaa
     1021 ttcgagtcgc agcactccgc cgacagacta gtaatctcca ttgcgggata ccgacgaggc
     1081 agaatgacgc tgcactgcgg ctcgggtaaa ctcgcagtgt gatatcatgg tattacgctt
     1141 gtcctggacg acccgaaacg cataagtaga cgggtgcgtt cgctcgagat cctgctggtg
     1201 gcggacagtg agctcgtcgc gcaagcgtta tcggcgacgc tgcgtcgcgc cggacatcgc
     1261 gtgaccgcgt cgacgacaat cgccggcacc gtcgagacgc tgatgcggcg aggccctcac
     1321 gtcgacatca tcatgaccag cacgcgattc gacagcgcgc tcggcgacga cgttgtccag
     1381 tacagccgaa agttcgccgc gaacgcgcgg ttcatcgtgc tgcgcgaggt ggccggccac
     1441 gtcgaggaca tcgagcgcgc gctgatccag caggcggtgc ccgaacggct cggcacggcg
     1501 cgcggctgga actgacgttc gaccggcgct gtgcgcgggc gctcgccagc gcgctccggc
     1561 gcgcactacg ggctgcgcgc aactagcatg gcggcgaggg ccggcgcgca acgcacgcgg
     1621 tgcggacgaa ctccagaagc ggcgcaaact gcagcggctt cttgaacagc ggatagccct
     1681 cgggcgcgag gtgcggcgag ctggagacga tcgcgaccgg gacgctggcg agcaccggcc
     1741 ggctggcgag gtacgccaga acgctggagc cgatgatccc cggcatcaga agatcgagga
     1801 tgatcgcgga gggcggttcg ttgtgctcca ggaacaggat cgccgccgca ccatccgtgg
     1861 cgacctcgac gcggaacccg gcgcgcgcga acaactcgca tagcaacatc cggatatccg
     1921 gatcatcatc gacgatcagc accgtgagac ggtccacaga ttgtgcatcg ctggcgatca
     1981 cacgtgatgc agctgcaatc gccagaccgc tgctatggcc cgatttcgcc tggagtatcg
     2041 tcgatctcag gatccaggca gatcggcgag cgccctacgg atcaggtccg ggggaaccga
     2101 ggctcgcggg atcttcgggc cagccccgcg ccgaccgcgg cgcgccggca gctcgaacca
     2161 cgagcgagga tcggcgtcga ggcccacgcc gtgcatgaag ttgtacaccg ccttgcgcag
     2221 cccggccgcg aacaagtcgt ggtcgcagcc ggtcgggtcg tcgaacgcga catcgttgcg
     2281 ggcaaacgtc gccggcgcgc cggcgcgcag ccggatgccg tagagcgccg gcgcgcgacc
     2341 gatcgggctg tggacggtgg cggtgaagcg gtgccagaac gccgactgca ggcagtccgc
     2401 ggcgaacagc tggcgcacgc gctccagcgc gtcgatcgtg tcctgcgcgg tctcggtcgg
     2461 gaacccgtac atcaggtagg cgtggaccat cacgccagcg tcgctgaacg cgcgggtcac
     2521 gcgggcgacc tgctcgacgg tgatcccctt gttcatcagc gcgagcaggc ggtcggacgc
     2581 gacctcgagc ccgccgctca ccgcgacgca gcccgagcgg gcgagcagct gggcgagctc
     2641 cggggtgaac gccgcctcga accgcacgtt gccccaccac gtgatcgtca cgccgcgcgc
     2701 gatcagccgc tcggccagcg cgcgcagcgc ggcgggtggc gcggcctcgt cgacgaagtg
     2761 gaacccgctc tgtccggtct cggcgatcat cgcctcgatc cggtcgacca gcaggtcggc
     2821 cggcgcgcgg tcgtagcgcg cgatgtagtc gagcgtgaca tcgcagaacg cgcactgctt
     2881 ccagtagcag ccgtgcgcga tcgtcagctt gttccaccgc ccgtcggccc acagccggtg
     2941 catcgggttg agcatctcga acagcgacag gtagcgatcg agcgcgagcc cggcgtagct
     3001 cggcgtgccg gcgtcgcgca gcgcgacgtc gcgcagccga tcgtcgctga ccagccggac
     3061 cgcgtcgcgc tcgcgcacga acgtccggta cagcggcgcc gcggggtcgg cgaggtggcg
     3121 gagcagcgcc agcagcggcg cctcgccgtc gtcgagcgtg acgtaatcga cgtagtcgaa
     3181 cacggccggc tcggcgagct cgcgcagctc ggtgttgacg tagccgccgc cgagcacgat
     3241 ccgggtcgcc ggggacaccc gcctgatcgc gcgcgcgatc cggaacgcgc catagacgtt
     3301 gccggggaac ggcacggtca ggccgaccac gtcggggcga tgccgcgcgc acagctcgcc
     3361 ggcgagctcg tcgagcagct cgtcgatcag cgtgggctcg ccctccagcg cgcggcgcag
     3421 cggatcgaag ctcggcgcgc tcgccgccag ctgctcggcg tagcgcgaca gcgcgaactc
     3481 gggcgcgatg ccgtcgcgga tcacgtcggc gaggtcgtcg atgtacaggc tcgcgaggta
     3541 cttggcgcga tcggcgatgc cgagcccgcc gaacgcccac gccagcggat cgtcatcgcc
     3601 cgcgccgggg ccctcggcga gcacggcaaa ccgcgggccc tcgggcaaca gctcgcggcc
     3661 ggcgatgcgc agcgcgagcg ccggatcgcg gccctggagg acccggatca ccgggtcgat
     3721 gg
//
LOCUS       DASVOR010001752         1611 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_134784,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001752 DASVOR010000000
VERSION     DASVOR010001752.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134784"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(178..>1611)
                     /locus_tag="VF516_32600"
     CDS             complement(178..>1611)
                     /locus_tag="VF516_32600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112532.1"
                     /translation="IEPRATGPVLGNGRADLVASQLAGPLQHVTQAIMFEGQSAADNR
                     RVLGFAFVPPDANSAVGATQFVQMVNVMIAVYSKRDGSLQLGPAPIHTLWQGFGGLCE
                     NGGTTPQFHDGGDPVVLYDHLADRWLVSQLQFDETFTHTAQCVAVSTSSDATGSYNRY
                     EFDYGANFPDYPKFGVWPDGYYNSINVFPGNKFAGAQACAIDRTAMLAGAPAAAICFQ
                     QPPTVSSLLPADLDGSRLPPAGAPNYYVGLADASHLNLFKFHADFATPSKSTFSGPTL
                     VPVADFNEICARANTVACIAEPEPGEKVDGLADRVMFRLAYRNFGDHESLVVNHTVLG
                     GALGGVRWYEIRNPGTAPAVFQQGTVVDPDTDFWLGSIAMDQAGDIALGFSAMSHTQF
                     SSIFVTGRTPADPPGKMFGPLVLARGSGVQVNSFKRWGDYSSMTVDPKDDCTFWYTQE
                     YYASTGSFNWATRVGAFKFDRCQPGAR"
BASE COUNT          237 a          559 c          570 g          245 t
ORIGIN      
        1 gcgcggccgc cgcccggggg gcgatcgccg acctaccagc cgacgtagat cgcgaccagc
       61 gcgtcgagct cgcgcgcctc gcccgcggga gcgctgccgc gcttgcggat caccgcgctg
      121 ccctcccagg ggcaacgcgc cgttcaggga gcctctgccc gggtgtcgtg cgcgcggtca
      181 cctggcgccg ggctggcagc ggtcgaactt gaacgcgccg acgcgggtcg cccagttgaa
      241 gctgccggtg ctcgcgtagt actcctgcgt gtaccagaac gtacagtcgt ccttcggatc
      301 gacggtcatg ctgctgtagt cgccccagcg cttgaacgag ttgacctgca cgccgctgcc
      361 gcgcgccagc acgagcgggc cgaacatctt gcccggcgga tcggcgggcg tgcggccggt
      421 gacgaagatg ctggagaact gggtatggct catcgcgctg aacccgagcg cgatgtcgcc
      481 cgcctggtcc atcgcgatgc tccccagcca gaaatcggtg tccggatcga ccaccgttcc
      541 ctgctggaac accgcgggcg ccgtccccgg attacggatc tcgtaccagc ggacgccgcc
      601 gagcgcgccg cccagcaccg tgtggttgac cacgagcgac tcgtgatctc cgaagttgcg
      661 ataggcgagc cggaacatca cccggtcggc caggccgtcg accttctcgc cgggctcggg
      721 ctcggcgatg cacgccacgg tgttcgcccg cgcgcagatc tcgttgaagt cggcgaccgg
      781 gaccagcgtc ggcccgctga acgtcgactt cgacggggtc gcgaagtcgg cgtggaactt
      841 gaagaggttc agatggctcg cgtcggccag cccgacgtag tagttgggcg cgccggcggg
      901 cggcagccgg ctgccgtcga ggtcggccgg cagcaggctc gacaccgtcg gcggctgctg
      961 gaagcagatc gcggcggccg gcgcgcccgc cagcatcgcg gtgcggtcga tcgcgcaggc
     1021 ctgcgcgccc gcgaacttgt tgcccggaaa cacgttgatc gagttgtagt acccgtccgg
     1081 ccagacaccg aatttcggat agtccgggaa gttggcgccg tagtcgaact cgtagcggtt
     1141 gtacgagccg gtcgcgtcgg agctggtcga caccgcgacg cactgcgcgg tgtgggtgaa
     1201 cgtctcgtcg aactgcagct ggctgaccag ccagcgatcg gcgaggtgat cgtagagcac
     1261 gaccggatcg ccgccgtcgt ggaactgcgg ggtcgtgccg ccgttctcgc acagcccgcc
     1321 gaacccctgc cacagcgtgt ggatcggcgc gggcccgagc tgcagcgagc cgtcgcgctt
     1381 gctgtacacc gcgatcatga cgttcaccat ctggacgaac tgcgtcgcgc cgaccgcact
     1441 gttggcgtcc ggcgggacga acgcgaaccc cagcacgcgc cggttgtccg ccgcgctctg
     1501 gccctcgaac atgatcgcct gggtcacatg ctgcagcggg ccggcgagct gggacgccac
     1561 caggtcggcc cgcccgttgc cgagcacggg accggtggcg cgcggctcga t
//
LOCUS       DASVOR010001753         4738 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_426496,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001753 DASVOR010000000
VERSION     DASVOR010001753.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4738
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426496"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..367
                     /locus_tag="VF516_32605"
     CDS             <1..367
                     /locus_tag="VF516_32605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112533.1"
                     /translation="DRGLLAAAADVLPQPGQGLAQAQTFTGDDTPVVEPGVVLVRLAR
                     TTPWPMVQAELKHLIGARGVSHATLRRVSPAGWVIGVATSDSIDRIASIVKKPPAADT
                     TVSVKIVGDLIEAALSGAP"
     gene            469..666
                     /locus_tag="VF516_32610"
     CDS             469..666
                     /locus_tag="VF516_32610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112534.1"
                     /translation="MGDAQVYVDGRFIAPLDALGGGVAVAPGAHRLELRHDDYFSSYL
                     EVQVARAARQKLAIDMAAMLP"
     gene            839..1402
                     /locus_tag="VF516_32615"
     CDS             839..1402
                     /locus_tag="VF516_32615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112535.1"
                     /translation="MRAKIQAPFVLAEIRALRASIFKKTQEVQTALAIADRVEQSYDL
                     HRADLFIPAGDTWCAILDPFAMTVLTGTLRRIGYEIFPQYVQILGIQPGNVKTAMDIK
                     VPADLVRTICEAYSRCVVGEDAGTLTPESHGSTVTVTDTTIIPCQLQMGVFLGAGKLT
                     GLFRENVLIEKRCRNKGDSVCSYEFVF"
     gene            complement(1384..2115)
                     /locus_tag="VF516_32620"
     CDS             complement(1384..2115)
                     /locus_tag="VF516_32620"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8112536.1"
                     /translation="MDWLLHDAERYRAQIQAGPFFKKLARTESPGDLKWVHQLIHQSR
                     EFTQALCLRYSLCRDKRYQPIFAEHAFEEADHPDQLIAWMEKHGFLEGVEPGDVPATQ
                     ETINNLAFCWRSAVHEPHDVQVVSLNVLSEGVALDFYSAVIPVLGKLDLLSGRYWKVH
                     REVDAHHLQMGLDLCGDVAPDSPTGMRYQRVLWHAANLYHQMLGSWVGERVEQLAPLW
                     GDSLSSRLILQHPRRPVRAVQNTNS"
     gene            complement(2857..4191)
                     /locus_tag="VF516_32625"
     CDS             complement(2857..4191)
                     /locus_tag="VF516_32625"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8112537.1"
                     /translation="MTPAEQPLSVRNEQVLVRQLSSVQQAMMAIGGAIGTGLFLSTGL
                     SVGIAGPAIIVSYVIVAGISLLLGRAVSEMAVAHPTPGAFGVHAALYVSPFAGYAVRV
                     SYWLMEVIATGGHLTAAATYMRFWFPEVHGAVWVLGFAVVLLYLNSRAVGRLARIEYW
                     LVLIKVTAVAVFVAIAILLVVGATGQPAVGVRNLTDHGFAPFGASGIWFGCCFGIYSF
                     IGCEIVGVTSGEAADPARSIPRAMRRSVLGLSAIYIVTITLLTAITPWQQLSVDKSPF
                     VTALSGHGIPAAAGVMNFVVLSAALSSSNANLYLISRTLFSLARGGFAPRSLGELSRR
                     GTPVNALLASGLGLAAAVVIEVLWEGTAYQILFALSLFGALFVWLMIFVTHIAFRGRR
                     LPFGSYLGLAAIAAILVSTWWVPLLRTTLLAGGPWLAILGIGYVVSRRRTPG"
     gene            complement(4226..>4738)
                     /locus_tag="VF516_32630"
     CDS             complement(4226..>4738)
                     /locus_tag="VF516_32630"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEX8112538.1"
                     /translation="RTVLAVLRAIPDLLWVLVFLVAIGLGPFAGALALGVHTGGVLGK
                     LYADTFEEVSPLPLRGLRAVGATPLQVLVWGAWPEARRMLASYTLLRWEMNLRSSTVV
                     GIAGGGGIGIALYNDIQLAAGSPLAFYPRVATLVLIVCLLIAATGWIGDAILRRLESA
                     SLAARRPPAR"
BASE COUNT          852 a         1604 c         1571 g          711 t
ORIGIN      
        1 cgatcgcggg ttgctcgcgg cagccgcgga cgtcctgccg cagccgggcc agggcctcgc
       61 tcaggcgcag acgttcaccg gcgatgacac gccggtggtc gagccgggcg tcgtgctcgt
      121 ccggctggcc aggaccacgc catggcccat ggtgcaggcc gagctcaagc acctgatcgg
      181 cgcgcgtggc gtatcgcatg cgacattgcg ccgcgtatcg cccgcgggct gggtgatcgg
      241 cgtggcgacc agcgattcga tcgatcggat cgcgtcgatc gtcaagaagc cgccggcggc
      301 cgatacgacg gtgtcggtga agatcgtcgg cgatctgatc gaagcggcgc tgtccggagc
      361 accatgactc gtggcctcct ggtggtgagc tggctcacgg cgtgcgcacc gggcacagca
      421 gcgaatcgcg tcacctcggg agcggcgatc atctatataa cgagtaacgt gggcgatgca
      481 caggtctacg tcgatggcag gttcatcgcg ccgctcgacg cgctcggcgg cggtgtcgcc
      541 gtcgcgccgg gcgcacatcg cctcgagctg cgccatgacg actatttttc gagctatctt
      601 gaggtccagg tcgcgcgtgc cgcgcgacag aaactcgcga tcgacatggc cgcaatgctt
      661 ccatgaccag ccccggcgca cggctcgccg gttgtagttc gaccctgcat gaagatgtct
      721 tgttcctgtt ccatccatgt gtcaccaggc tggatgcacg tttccgtccc ttatgcggac
      781 cgtgtagagt aatgtcagcc ggggaggtgg tatgtcgttt cctttggatc gcaagaacgt
      841 gcgcgcgaag atccaggccc ccttcgtgct cgcagagatc cgggcgttgc gcgcctcgat
      901 cttcaagaag acgcaggagg tccagaccgc cctggcgatc gcggacaggg tcgagcagtc
      961 ctacgatctg caccgcgcag acctgttcat ccccgccggc gacacctggt gcgcgatcct
     1021 cgatccgttc gcgatgaccg tcctgaccgg cacgctgcgg cggatcgggt acgagatctt
     1081 cccccagtac gtgcagatcc tgggcatcca gcccggcaac gtgaagaccg cgatggacat
     1141 caaggtcccg gcggacctcg tacgcacgat ctgcgaggcc tacagccggt gcgtggtcgg
     1201 tgaggacgcc ggcacgttga cgccggagag ccatggctcg accgtcacgg tgaccgacac
     1261 cacgatcatc ccgtgccagc tccagatggg cgtgttcctc ggcgccggca agctgaccgg
     1321 cctgttccgc gagaacgtgc tgatcgagaa gcgctgccgc aacaagggcg acagtgtgtg
     1381 cagctacgag ttcgtgttct gaaccgcgcg gaccggtcgc cgcgggtgct gcaggatcag
     1441 gcggctcgac aggctgtcgc cccacagcgg ggcgagctgc tccacgcgct cgccgaccca
     1501 cgagccgagc atctggtggt agaggttcgc cgcgtgccac agcacccgct ggtagcgcat
     1561 gccggtcggt gaatccggcg ccacgtcgcc gcagaggtcg agccccatct ggaggtggtg
     1621 ggcgtcgacc tcgcgatgca ccttccagta ccgcccgctc aggagatcga gcttgccgag
     1681 caccgggatc accgccgagt agaagtcgag cgcgacgccc tcgctcagga cgttgagcga
     1741 gacgacctgg acgtcgtgcg gctcgtggac cgcgctgcgc cagcagaacg ccaggttgtt
     1801 gatcgtctcc tgggtcgccg ggacgtcgcc gggctccacc ccctcgagaa acccgtgctt
     1861 ctccatccac gcgatgagct gatcggggtg atcggcctcc tcgaaggcat gctcggcgaa
     1921 gatcggctgg taccgcttgt cccggcacag cgagtagcgc aggcacagcg cctgcgtgaa
     1981 ctcgcgcgac tggtggatca gctggtggac ccacttgagg tcacccggcg attcggtccg
     2041 cgcgagcttc ttgaagaacg gacccgcttg gatctgtgcg cggtatctct cggcgtcgtg
     2101 gagcagccag tccataaaat caatcctcgt tctgtggagc tctgcggttg gggtcgtaat
     2161 cgatgctgtc tagggaacac gccgcccggc gcgcgccggt ggaaagcccg aagccccaac
     2221 ccgcccctcc gcccaggctg gccgaccgcg cgatgaggtg tggtgccgtc cgtgcgccat
     2281 gcgcccaccg cagcaattcg cggcaagttg aggcatgccc gacccgaaca cgcgctgccg
     2341 gtgagtgagc cctgccgagg atcgctcccc cgcagcggtt cgcctcgcgc gctgttcgtg
     2401 tcgtatgccg gacgaccatc aggtctccca gactactcgt gctgagagtt atccgcatag
     2461 aaataccgta cgggcagaat cttcttatgt gctatgaatt acattcatat atgaaatgga
     2521 taactctttc tcgaaattgc catcaataat agtaatataa catacagaat tcagaaaact
     2581 gatggcgctt cgacctcaaa cctcagagcc tagatatctg aaaaaattgg cctcaggcag
     2641 gtttcgcgac cgactgtgca agcatcgtcc agccgcgttt cagcacgctc ggatatggag
     2701 cagcatccga cacgcggggg gacgcgctgg agagagaaaa tttcaccagg gaccgcgctc
     2761 cggagcacag tgcgtcgccg ccccccgccg cctcgccgtc ctacccgcgt ctgctcgcgg
     2821 atatggaaca catttcacga ccgtgtggtg acggcatcag ccgggtgtgc gccggcgaga
     2881 caccacgtag ccaatgccga ggattgccag ccatggccct cctgcgagca gcgtggtcct
     2941 caggaggggt acccaccacg tggatacgag gatcgctgcg atcgcggcga gcccgagata
     3001 ggacccgaac gggagccttc gcccgcgaaa ggcgatgtgg gtgacgaaga tcatcaacca
     3061 gacgaacagc gcgccgaaca gcgacagcgc gaacaggatc tggtacgcgg tgccctccca
     3121 cagcacctcg atcaccaccg cggcggccag gccaagcccc gaggccagca gcgcgttgac
     3181 tggagttccg cggcggctca gctcgccgag cgagcgcggt gcgaaccccc cgcgcgcgag
     3241 cgagaacagg gtgcgcgaga tcaggtagag gttggcgttg gagctcgaca gcgccgcgct
     3301 cagcacgacg aagttcatca cgcccgcggc cgccgggatg ccgtgcccgg acagcgccgt
     3361 gacgaacggg ctcttgtcga cgctgagctg ctgccacggc gtgatcgcgg tcagcagcgt
     3421 gatcgtgacg atgtagatcg ccgacaggcc gagcaccgag cggcgcatcg cgcgcgggat
     3481 cgagcgcgcc ggatcggcgg cctcccccga ggtgacgccg acgatctcgc acccgatgaa
     3541 cgagtagatc ccgaagcagc agccgaacca gatccccgag gcgccgaacg gcgcgaaccc
     3601 gtggtcggtg aggttgcgca cgccgaccgc gggctggccg gtggcgccca cgacgagcaa
     3661 gatggcgatc gcgacaaaca ccgcgaccgc ggtgaccttg atcagcacca gccagtactc
     3721 gatccgggcg agccggccga ccgcccgcga gttcaggtac agcagcacca cggcgaaccc
     3781 gagcacccac accgcgccgt gcacctcggg gaaccagaac cgcatgtagg tcgccgcggc
     3841 cgtcaggtgg ccgccggtcg cgatcacctc catcagccag tagctcaccc gcaccgcata
     3901 gccggcgaac ggcgagacgt agagggcggc gtggaccccg aacgcgccgg gggtcgggtg
     3961 ggccaccgcc atctccgaca ccgcgcggcc gagcagcagc gagatcccgg ccacgatcac
     4021 gtacgacacg atgatcgccg gcccggcgat gccgaccgac agcccggtcg acaggaacag
     4081 gccggttccg atcgcaccac cgatcgccat catcgcctgc tggaccgacg acagctggcg
     4141 caccagcacc tgctcgttgc ggaccgacag gggttgttcg gcgggggtca tggcttcggg
     4201 ccgccgcgac ggcgcgcgca gtttatcacc gggccggggg gcgccgcgcc gccaggctcg
     4261 ccgattccag ccggcgcagg atcgcatcgc cgatccagcc ggtcgccgcg atcagcaggc
     4321 agacgatcag gaccagcgtg gcgacccgcg ggtagaacgc gagcggcgag ccggccgcga
     4381 gctggatgtc gttgtacagc gcgatgccga tgccgccgcc gccggcgatg ccgaccaccg
     4441 tcgagctgcg caggttcatc tcccagcgca gcagcgtgta gctggccagc atgcgccgcg
     4501 cctcgggcca cgcgccccac accaggacct ggagcggcgt cgcaccgacc gcgcgcagcc
     4561 cgcgcagcgg cagcggcgac acctcctcga aggtgtcggc gtacagcttg ccgagcacgc
     4621 cgccggtgtg gacgccgagc gccagcgcgc cggcgaacgg gcccaggccg atcgcgacga
     4681 ggaacaccag cacccacagc agatcgggga tcgcacgcag caccgccagc acggtccg
//
LOCUS       DASVOR010001754         5412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_310650,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001754 DASVOR010000000
VERSION     DASVOR010001754.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310650"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VF516_32635"
     CDS             <1..242
                     /locus_tag="VF516_32635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112539.1"
                     /translation="EAARALFGLGRIDGWAFDLEILALARRTGFAIAEVGVEWTDDRR
                     SRVHPLQDMWKVMRETLTIRKNLRRGVYRPLPAAA"
     gene            complement(338..763)
                     /locus_tag="VF516_32640"
     CDS             complement(338..763)
                     /locus_tag="VF516_32640"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8112540.1"
                     /translation="MIHFLDTSALVKRYVTEPGSDQVRRLLRRAVDVALARITEAEAC
                     AAIARAARMNVLTKDDRDRAFQQIAEDVARARVVEIRRSVVSTVHGLVVRWPLRGYDA
                     VQLSCALRLQSENLPITFWCADSELASAARGEGMRVTVV"
     gene            complement(760..1035)
                     /locus_tag="VF516_32645"
     CDS             complement(760..1035)
                     /locus_tag="VF516_32645"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEX8112541.1"
                     /translation="MQIGTRELKNRLSHYLRQVRESKESIYVTDRGEIVAELRPVAGG
                     SEEGEVLLALAARGELSPGNGRRFRSFKPIKPRRPVSLAGIVLEDRG"
     gene            complement(1343..2764)
                     /locus_tag="VF516_32650"
     CDS             complement(1343..2764)
                     /locus_tag="VF516_32650"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX8112542.1"
                     /translation="MFKYDVQGTDERIAFVDENSRILPAFEATSEQGVFAYLSVRTTS
                     WDFDGERTDTNDALSFFLAIVLRTLDISCALWDVPNPFSRVPSELYARYITIAQPTRS
                     NYSLDEQGIAQIHKLLFGVRLFETNIGQLLEWDTSINQMRDSTYGWQDRTTKTWARKV
                     AQVTGETLRSRKVQYNFRKNPCWLYYRTTGKNVAVYRSSGFAARMYDFIDTLSSIRTT
                     QAVNATAYSSESSRNVVPNASARIAQDILARLEPNMPQSLLFFPIETHFLAIGTEHLV
                     SVRCDCGLKAFEEERERVRARRKVEEDIFFPGITFTWAAKIDDRRFELLIRDLLAVEP
                     GIRWVRKIGQTREGDEGRDLICEWITPPAPGEPIDDRKPPVATRRVIIQCKASAKSVG
                     KSMVQDVYDTIKHHECNGYFLAVSSQLSVPLTRFLEGLRKGSEIWTDWWTRSELEDRL
                     RNRPEILARYPDIIRQLPQSGGT"
     gene            complement(3099..3719)
                     /locus_tag="VF516_32655"
     CDS             complement(3099..3719)
                     /locus_tag="VF516_32655"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8112543.1"
                     /translation="MLKIWGRISSINVRKVVLVAQELGVPFERIDAGGAFGIVKTPEF
                     LARNPNGLIPLIEDDQVELWESNAIVRYLCARHAADTLYPLALPARFDAERWMDWQQT
                     TLGPAGRTAFIQWIRTPAEQRNHEAIAQSVAATEPLLALLDQHLSHRPFMAGDRLTMA
                     DFPIACEIHRWINLPQPRTERPHLDRWYAGMLARPSARGVLDLALS"
     gene            3811..4162
                     /gene="rnpB"
                     /locus_tag="VF516_32660"
     ncRNA           3811..4162
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VF516_32660"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4304..>5412
                     /locus_tag="VF516_32665"
     CDS             4304..>5412
                     /locus_tag="VF516_32665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112544.1"
                     /translation="MEQRSPVHEIVNELWRALHELGSPATTGEIERWGFSIHAALSAW
                     GREFHNHDHVIELVTGSEPLEVIAALYHDAVYIQVDQGPPRSMREELTSVLAQGADGW
                     RVLPSAARPVTGDVLHVFGRKVGDVVAPTTGLNELSSALVAAIQLEHALSREQRLVIA
                     ACIEQTIPFRVDPVPALHGRLGELGLSGTALDEAICSAVRVGNRDVENFADNDAARFL
                     DNTWKLLPESNPALHSPMVYTIRDYRIALLKMESFLAWLPAERVFHSWNDEPRPEVHA
                     RRVARATGNIELAVRYLRAKLYSIGLVEAIAELTGGDVPVDYFMGGVKSARRPAMKTI
                     EQFLPALPDADDLDPPLHRLLAEGRASESSFDTGPS"
BASE COUNT          976 a         1689 c         1783 g          964 t
ORIGIN      
        1 ccgaggccgc ccgcgccctg ttcggcctgg gccggatcga cggctgggcg ttcgacctcg
       61 agatcctcgc gctcgcccgc cgcaccggct tcgcgatcgc cgaggtcggc gtcgagtgga
      121 ccgacgaccg ccggtcgcgc gtccacccgc tgcaggacat gtggaaggtc atgcgcgaga
      181 ccctgaccat ccgcaagaac ctgcggcgcg gcgtctaccg cccgctgccc gccgccgcgt
      241 gagccggcgc ggcggccgat cgcgaaggcg tgaaccgacc gatcgcgtac cgcagccccg
      301 ctcatcgccg cctgcgtgcg cgtgctcact ggaatcgtca gacgaccgtg acgcgcatcc
      361 cttcaccgcg agctgcgctg gcaagctcgc tatccgcaca ccagaacgtg atcgggaggt
      421 tctcggactg caaccggagc gcgcaggaca gctggaccgc gtcgtatccg cgcaagggcc
      481 atcgcaccac gaggccgtgc acggtgctca ccacgctcct ccggatctcg acaacacggg
      541 ctctggcgac gtcctcggcg atctgctgga acgctcggtc gcgatcgtcc ttggtgagca
      601 cgttcattcg cgcggctcgc gcgatcgccg cgcatgcctc ggcttccgtg atgcgggcca
      661 acgcgacatc aaccgcacgc cgaagcagcc gtcgaacctg atcggatccc ggctcggtga
      721 cgtagcgttt gacgagcgcg ctggtgtcga gaaaatggat catccgcggt cttcgagcac
      781 gatgcctgcg agcgagaccg gccgcctggg tttgattggc ttgaagctcc tgaaccggcg
      841 accattgccc ggcgagagct cgccccttgc ggcgagcgcg agcaagacct cgccttcctc
      901 gctgccgccg gcgaccggac gaagctcggc gacgatctcg ccccgatcgg tcacgtagat
      961 cgattccttc gactcgcgga cctgccgcag atagtggctg aggcggttct tgagctcgcg
     1021 tgttccgatc tgcacgacct caaggtagcg cttgtagcca catcgagcaa cagcctgagg
     1081 gggacctacc gcagcattcg tcgcgcaccg cagcgttgcc gttcatggtc aacgtcagct
     1141 tgccctctgg ccgcgtgagc tgaccggcgc gaaggatcac gtggcgaagc gtgtccaggt
     1201 tgcgaaaagc cccgcgtgcg aggttcgcgg gtcgcgcaga cttcgcgacc cggctgacat
     1261 ttggagctcg cggcagtggt tgtgcgtgag caccgaggtt gcgtaaagat ctggttaggc
     1321 ctcattctta gtgttcatgc aattaggtcc ctccagattg cggtaactgg cggatgatgt
     1381 caggataacg cgcaagaatc tccggtctat tccgaagtcg gtcctcgagt tcgctgcgag
     1441 tccaccaatc tgtccagatt tccgatccct tacgcagacc ctctaagaat ctggtcaaag
     1501 gcacgcttag ctgtgaggat actgccagga aatagccatt gcattcatga tgtttgatcg
     1561 tatcgtaaac atcttgtacc attgactttc cgacagactt cgcggatgcc ttgcactgaa
     1621 tgattactcg tcgagtcgcg acaggcggct tccgatcgtc gattggctcg cctggcgctg
     1681 gcggtgtgat ccattcgcaa atcagatcgc ggccctcatc gccttctcgt gtctgtccaa
     1741 tctttcgcac ccatcggata ccgggctcaa cagctaagag atcgcggatg agcagttcga
     1801 aacgtcgatc gtcgattttg gctgcccacg tgaacgttat gcctgggaag aagatgtcct
     1861 cttctacttt ccgtcgagca cgcacgcgct cccgttcctc ctcaaatgcc ttcagaccgc
     1921 agtcgcatcg aacggagaca aggtgttcag ttccaatggc cagaaaatga gtttcgatgg
     1981 gaaagaatag cagcgactga ggcatattgg gttcaagcct tgcgaggata tcttgcgcga
     2041 tgcgagctga cgcattcggc acaacatttc gcgaagactc agaggagtat gctgtggcgt
     2101 ttactgcctg cgtcgttcga atactcgaca aggtatcgat gaaatcatac attcgcgctg
     2161 cgaacccaga cgatcgatac accgcgacgt tcttgccggt cgtgcgataa tagagccaac
     2221 atggattctt cctgaagttg tactgaacct tacggctgcg caaggtctcg ccagtaactt
     2281 gtgcaacctt gcgtgcccac gttttcgttg ttcggtcctg ccaaccgtag gtgctatcgc
     2341 gcatctgatt gatacttgta tcccattcta acaactgccc aatatttgtt tcgaacaacc
     2401 tgactccgaa tagtaattta tggatctgcg caattccttg ttcatcgaga ctgtaattgg
     2461 atctcgtcgg ttgagcaatt gtaatatatc ttgcatagag ttcagatgga acacggctga
     2521 acggattagg tacatcccaa agtgcgcatg aaatatcaag agttcgaaga acaatcgcaa
     2581 ggaagaacga caaagcgtcg tttgtgtcgg tgcgctcacc atcgaagtcc cacgacgtcg
     2641 tgcgaacgct tagataggca aacacgcctt gctcgctggt cgcttcgaat gcgggcagga
     2701 tccgagaatt ctcatcgaca aaagcgatgc gctcgtctgt cccttggacg tcgtatttga
     2761 acatcttcgc caagggctct agcttcgctt tcatcgcgac ctccgccttt taggtgtgtg
     2821 ctattcatgc gccaaactat tttggggaca agtacatggt cagacgatat cggacaatcc
     2881 tgtcataggg agaagatcta tgatgtcacc aagcctactc cgagaagggc gcgccggcgc
     2941 tcatgtccgc ggcaacttca cgtaaccaga gggtatccgc gtctccccga agcgcatccg
     3001 ctcctagagc ggcgctgcgg cggtccggca gtcaacgatc accagcgcac cggcgtcaag
     3061 cccaggccgc cgcaggcagt gacctcacgc cggaccggct acgacagcgc gagatcgagg
     3121 acgccgcggg cggacgggcg ggcgagcatg ccggcgtacc agcggtcgag gtgggggcgc
     3181 tcggtgcgcg gctgaggcag gttgatccag cggtggatct cgcaggcgat cgggaagtcg
     3241 gccatggtga ggcgatcacc ggccatgaac ggccggtgcg agaggtgctg atcgagcagc
     3301 gcgagcaggg gctcggtggc ggcgaccgac tgggcgatcg cctcgtggtt gcgctgctcg
     3361 gcgggggtgc ggatccactg gatgaaggca gtgcggccgg ccgggccgag ggtggtctgc
     3421 tgccagtcca tccagcgctc ggcgtcgaac cgcgccggca aggcgagcgg gtagagggtg
     3481 tcggcggcgt ggcgggcgca caggtagcgg acgatggcgt tggactccca gagctcgacc
     3541 tgatcgtcct cgatgagggg gatcaggccg ttggggttgc gggcgagaaa ctccggcgtc
     3601 ttgacgatgc cgaacgcgcc gccggcgtcg atccgctcga acgggacgcc gagctcctgc
     3661 gcgacgagca cgaccttgcg cacgttgatc gacgagatgc ggccccagat cttcagcatg
     3721 cgagggcagc gtagcgcacc cggtggccgg cgatgcggat ccgtttgaca gggcggcgag
     3781 cgggctgtac gtatgttccg cccgttccga cggagaggtc gaggcggccg ctggcggcgg
     3841 cgcgcaagcg ccggcgcgag aggaaagtcc gagcttcaca gggcagggtg ctggttaacg
     3901 accagtcgag gtgactcgca ggacagtgca acagagagca gaccgcccat cgcgagatgg
     3961 gtaagggtga aacggtgcgg taagagcgca ccgcctctcc ggtgacggag agggcacggc
     4021 aaaccccacc cgaagcaaga ccagacaggg gtcgcgttgc ccgcgccatg gccccgggtt
     4081 ggtcgctgga gcccgtcggt aacggcgggc ctagatgaat ggtcgccaca cacagaactc
     4141 ggcttacgga cctctccgcc ggggcgggca cctgtgcgcc gagcgcgtgg atgcgcgccg
     4201 gagccacgcc ggagccacac tcgatgcagc gcggtgcggc gggcggcgcc cccgggggcc
     4261 ggcgccatcg cggccggcac gcgtgatacg gtggaccggg gccatggagc agcgctcgcc
     4321 ggtacacgag atcgtgaacg agctgtggcg cgcgctgcac gagctcggct cgccggcgac
     4381 gaccggcgag atcgagcgct gggggttctc gatccacgcg gcgctgtcgg cctggggccg
     4441 cgagttccac aaccacgacc acgtgatcga actggtcacc ggcagcgagc cgctcgaggt
     4501 gatcgcggcg ctgtaccacg acgcggtcta catccaggtc gaccagggcc cgccgcgctc
     4561 gatgcgcgag gagctgacca gcgtgctggc gcagggcgcc gacggctggc gcgtgctgcc
     4621 gtcggcggcg cgcccggtga ccggcgacgt gctccacgtg ttcggccgga aggtaggtga
     4681 tgtggtcgcg ccgaccaccg ggctcaacga gctgtcgtcg gcgctggtcg cggcgatcca
     4741 gctcgagcac gcgctgtcgc gcgagcagcg gctggtgatc gcggcgtgca tcgagcagac
     4801 gatcccgttc cgggtcgatc cggtgcccgc gctgcacggc cggctcggcg agctcgggct
     4861 gtccgggacg gcgctcgacg aggcgatctg cagcgcggtc cgggtcggca accgcgacgt
     4921 cgagaacttc gccgacaacg acgcggcgcg gttcctcgac aacacctgga agctgttgcc
     4981 cgagagcaac ccggcgctgc actcgccgat ggtgtacacc atccgcgact accggatcgc
     5041 gctgctcaag atggagagct tcctggcctg gctgccggcc gaacgggtgt tccatagctg
     5101 gaacgacgag ccgcggcccg aggtccacgc ccgccgggtc gcgcgcgcga cgggcaacat
     5161 cgagctcgcg gtccgctacc tgcgggccaa gctgtactcg atcgggctgg tcgaggcgat
     5221 cgccgagctg accggcggcg acgtgccggt cgactacttc atgggcgggg tcaagtcggc
     5281 gcgccggccg gcgatgaaga cgatcgagca gttcctgccg gcgctgcccg atgccgatga
     5341 cctcgatccg ccgctgcacc ggctgctcgc cgagggccgc gcgtcggagt cgagcttcga
     5401 caccgggccg tc
//
LOCUS       DASVOR010001755         5562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_47598,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001755 DASVOR010000000
VERSION     DASVOR010001755.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5562
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47598"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..1560
                     /locus_tag="VF516_32670"
     CDS             280..1560
                     /locus_tag="VF516_32670"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112545.1"
                     /translation="MFADCTLRDGEQAPGVFFTLEEKLALAAMLDAAGVDLIDAGMPS
                     VSKEERAALTALVNRNLRARVAATVRALRSDIDLAVECGVKEVFLFMPVSPQHLQHKF
                     GIDLEKARTRIDDAVGYAVERGLRVHFVAEDSVRADPQQIAQVFDRVCELGAFSAIIC
                     DTVGVMTPQTMERYVRTLRAHMTRSLTLGIHCHNDYGLGTANTLAAVSAGCTLVTSTV
                     NGLGERAGNAVFEEVVCALDDLYDAEIAIDKSMLSPLSQAVAQASGMFIVPTKPVVGH
                     NVFRHESGVHVDGMLKNPATYESICPEPLGRTHEYVLGKHSGSGLVNVLLAEKGIVCT
                     PEQTDRILERIKAVKVSTNKAPLRVMAARLDELWSRHLSFCEEDFWAIVYEETSRGLV
                     RSRGRGILLACGTKQMPAAEPDAEPATAHNPAAL"
     gene            1557..2888
                     /locus_tag="VF516_32675"
     CDS             1557..2888
                     /locus_tag="VF516_32675"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase/3-isopropylmalate dehydratase large
                     subunit family protein"
                     /protein_id="HEX8112546.1"
                     /translation="MTPQTLTAKLLRRRTGEPIIAGSEFFRIPVDLVLGHDATIALLI
                     ERFQRAGRRIWDPGRCFFAADHFTPPSTPERADILHRYLAFMKQECVPQDLVFRGISH
                     QLLVEDRRCQPGMVICGADSHTVMAGALGAFACGMGSTDILALLLTGEVLLAVPESIR
                     IDFEGALPEWLSGKDLALEIIRRLGEGGAVDRALEYHDRTRGGLTMDSRFAVANMAVE
                     AGATNGVFVPDDALRGYLAERDGAAGPVPGFEGAWLEPDDGARYVQQLRIDVAALCPL
                     VALPGDLGRIVPADEVEPRAVQQVFLGSCAGGRIEDLAVAARVLRGRRVAPGVRLVVT
                     PASQAVFHACLRNGIFEVLSEAGAMITNASCGACGGIDKGLIGPGEACVSTSNRNFRG
                     RMGSPDGLIYLASPLTAAAAALTGTICDPRTVLDDRVAADLHAAVAPGGRA"
     gene            2885..3418
                     /locus_tag="VF516_32680"
     CDS             2885..3418
                     /locus_tag="VF516_32680"
                     /inference="COORDINATES: protein motif:HMM:NF012897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112547.1"
                     /translation="MRIHGKALILGDNVNTDELHPSRFFSLDDRTVRSGFLQAAAGYE
                     QVGGSDLSGRVIVAGDNFGCGSSRETGARAFLLAGVRAVIARSLARIFSRNVRNLGLA
                     AVECPALPALPPDIEVELDLARWRLLVPAGSLDLPVTPLDPFWAAVLQAGGLLPFLGL
                     GEATGPRGTLPVYGGRP"
     gene            3427..4599
                     /locus_tag="VF516_32685"
     CDS             3427..4599
                     /locus_tag="VF516_32685"
                     /inference="COORDINATES: protein motif:HMM:NF016338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112548.1"
                     /translation="MNSERCRTLCERVAAFIDGGEDGEDSKQQFEALALEVFAFQFEA
                     IKPYQRLCRGLGRTPDHVTSWREIPAVPAVAFKQYSLFAGAPEAIIRTFRSSGTTEPS
                     RSSRAHFSRDGLELMDVAIAAGARSRLFPDGRSTRILVLAPPPEQVPHMIMAHGMAHL
                     IREYGLPGSRFLAGPRGIDFAALWSELDGCQWKGIPVSLLGSSFGFVHFFDWMEREGC
                     RLELPAGSRLLDAGGYKGKSREIGREAFVTWAAQLCGLRPARVINLLGMTELASQIYD
                     RLTPGAADVARMKLPPHWVRTTVVEPRCPGQRGPEPVGDGEIGLLRHVDLANIERPIA
                     IQSEDVGRTVTASVDGELRRGFEILGRARGAEPRGCSLTAEDIAPHSGEAAQSACA"
     gene            4610..>5562
                     /locus_tag="VF516_32690"
     CDS             4610..>5562
                     /locus_tag="VF516_32690"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEX8112549.1"
                     /translation="MIAPRTRTANAGPTHVACWLPPALREAIDAMPGGATPYRKRYDH
                     DDQHLEVEFERLSPDALDQVVAFLRRRRREVLARRTVGEIVDVLERAAVRWLDPAYPP
                     RAAAVLEIAFITGFSPEMVGHAIDEEQASSRGPHLTLALRSELGDPEFLDGFRPNPRT
                     GGLSRAHGPGLVGAIFSSNIPALPHLEIMRSFLVKSACLGRVSAGEPIFLRRYAETLH
                     ELDPELASCLAIVYWERGDDELEARFLRSIDYLVAYGGDAQIQRLMASRPGALDATWH
                     GHRLGFTYVTREALAAGSDLRRLARAIAYDFAIFDGFACLCP"
BASE COUNT          776 a         1989 c         1986 g          811 t
ORIGIN      
        1 tacaaacaat cccatcggaa aattactgtg agcaaataat ccagtgtaag cggaaaggga
       61 ctccttcgaa gctatgtctt ggaagcacac cccgtgccaa aggtgagtca atagtcagga
      121 ttctcggtgc aagccagcgg acgagccaga cctccggaaa ttgccaggcc cactcgaccg
      181 cactcaccca cagaaaactg gctctcgacg cgtcgggccg tgcagccgcc atccacttct
      241 tcaatccgtc tggaggtagc agtgactgat tcaagaatcg tgttcgcaga ttgcacattg
      301 cgcgatggcg agcaggcccc gggggtgttc ttcacgctgg aggagaagct ggcgctcgcc
      361 gcgatgctcg atgcggcggg ggtcgatctc atcgatgcgg ggatgccgag tgtatcgaaa
      421 gaggagcgcg ccgcgctgac ggccctggtc aaccggaacc ttcgcgcacg ggtggccgcg
      481 acggtccggg cgctgcgatc cgacatcgat ctcgcggtcg agtgtggcgt gaaggaggtg
      541 ttcctgttca tgcctgtgag cccgcagcac ctccagcaca agttcggcat cgatctggag
      601 aaggcccgga cccggatcga cgacgcggtc ggctacgcgg tcgagcgcgg gctgcgcgtc
      661 cacttcgtgg ccgaggactc ggtccgcgcc gatccccagc agatcgcgca ggtgttcgat
      721 cgggtctgcg agctcggcgc gttcagcgcg atcatctgcg acacggtcgg cgtgatgacg
      781 ccgcagacca tggagcgcta cgtccggacg ctgcgcgccc acatgacccg ctcgctgacc
      841 ctcgggatcc actgccacaa cgattacggg ctcggcaccg cgaacacgct ggcggcggtc
      901 agcgcgggct gcacgctggt gacctcgacg gtcaatggcc tcggcgagcg cgccggcaac
      961 gccgtgttcg aggaggtggt gtgcgcgctc gatgacctct acgacgcgga gatcgcgatc
     1021 gacaagtcga tgctgagccc gctgtcccag gcggtggcgc aggcctcggg catgttcatc
     1081 gtgccgacca agccggtggt cggccacaac gtgttccgcc acgagtccgg ggtccacgtc
     1141 gacggcatgc tcaagaaccc ggcgacctac gagtcgatct gccccgagcc gctcggccgc
     1201 acccacgagt acgtgctcgg caagcactcc ggcagcggcc tcgtcaacgt gctgctggcg
     1261 gagaagggga tcgtgtgcac gccggagcag accgaccgca tcctcgagcg gatcaaggcg
     1321 gtcaaggtct cgaccaacaa ggcgccgctg cgcgtcatgg cggcgcggct cgacgagctg
     1381 tggtcgcgcc acctgtcgtt ctgcgaggag gacttctggg caatcgtcta tgaagagacc
     1441 tcgcgcggat tagtccgctc gcgaggacgg ggcatcctgc tcgcctgcgg gaccaagcag
     1501 atgccggcgg cagaacccga cgccgagccc gccaccgcgc acaacccggc cgcgctatga
     1561 cgccacagac gctgaccgcg aagttgctgc ggcggcgcac cggggagccg atcatcgccg
     1621 gaagcgagtt ctttcgcatc ccggtcgacc tcgtgctcgg ccacgacgcg acgatcgcgc
     1681 tcctgatcga gcggttccag cgcgcggggc gccggatctg ggatcccggg cgctgcttct
     1741 tcgccgccga tcacttcacg ccgccgtcga ccccggagcg cgccgacatc ctgcatcgct
     1801 acctcgcgtt catgaagcag gagtgcgtgc cgcaagacct ggtgttccgc ggcatcagcc
     1861 accagctgct ggtcgaggac cggcggtgcc agcccggcat ggtgatctgc ggcgcggact
     1921 cgcacacggt gatggccggc gcgctcggcg cgttcgcgtg cggcatgggc tcgaccgaca
     1981 tcctcgcgct gctgctcacc ggcgaggtcc tgctggcggt ccccgagtcg atccggatcg
     2041 acttcgaggg cgcgctgccc gagtggctgt ccggcaagga cctcgccctg gagatcatcc
     2101 ggcggctcgg cgagggcggc gcggtcgatc gcgcgctcga gtaccacgat cggacgcgcg
     2161 gcgggctgac gatggacagc cggttcgcgg tcgccaacat ggcggtcgag gccggcgcga
     2221 ccaacggcgt gttcgtcccc gacgacgccc tgcgcggcta cctcgcggag cgcgacggcg
     2281 cggcgggccc ggtccccggg ttcgagggcg cgtggctaga gcccgacgac ggcgcgcgct
     2341 acgtccagca gctgcggatc gacgtcgcgg cgctctgccc gctggtcgcg ctgcccgggg
     2401 acctgggccg gatcgtcccg gccgacgagg tcgagccgcg cgcggtccag caggtgttcc
     2461 tcggatcgtg cgcgggcggc cggatcgagg acctcgcggt cgcggcgcgc gtgctgcgcg
     2521 gccggcgggt ggcgcccggc gtccggctgg tcgtgacgcc ggcgagccag gcggtgttcc
     2581 acgcctgcct gcgcaacggc atcttcgagg tgctgagcga ggccggcgcg atgatcacca
     2641 acgcgtcgtg cggcgcgtgc ggcgggatcg acaaggggct gatcggcccg ggcgaggcct
     2701 gcgtgtcgac ctcgaaccgc aacttccgcg gccgcatggg ctcgcccgac ggcctgatct
     2761 acctcgcgtc gccgctgacc gcggccgccg cggcgctgac cgggacgatc tgcgatccgc
     2821 ggaccgtgct cgacgatcgc gtcgccgccg acctgcacgc ggcggtcgct cccggggggc
     2881 gcgcatgagg atccacggca aggccctgat cctgggcgac aacgtcaaca ccgacgagct
     2941 ccatccgtcg cggttcttct cgctcgacga ccgcacggtg cgctccgggt tcctccaggc
     3001 cgcggccggc tacgagcagg tcggcggcag cgacctgtcc ggccgggtga tcgtcgccgg
     3061 cgacaacttc gggtgcggct cgagccggga gaccggcgcg cgcgcgttcc tgctcgccgg
     3121 cgtgcgcgcg gtgatcgccc gctcgctcgc gcggatcttc agccgcaacg tgcgcaacct
     3181 cggcctggcc gcggtggagt gcccggcgct gcccgcgctg ccgcccgaca tcgaggtcga
     3241 gctcgatctc gcgcgctggc ggctgctcgt gccggccggg tcgctcgatc tgccggtgac
     3301 cccgctcgac ccgttctggg cggcggtgct ccaggccggc ggcctgttgc cgttcctcgg
     3361 cctgggcgag gcgaccggcc cgcgcggcac gctgccggtg tacggaggcc ggccgtgatc
     3421 gccgcgatga acagcgaacg gtgccgcacg ctgtgcgagc gggtggccgc gttcatcgac
     3481 ggcggcgagg acggcgagga cagcaagcag cagttcgagg ccctggcgct cgaggtgttc
     3541 gcgttccagt tcgaggcgat caagccgtac cagcggctgt gccgcggcct cggccgcacc
     3601 ccggaccacg tgacgtcgtg gcgcgagatc ccggcggtcc cggcggtggc gttcaagcag
     3661 tactcgctgt ttgccggcgc cccggaggcg atcatccgca cgttccgcag cagcggaacc
     3721 acggagccca gccggagcag ccgggcgcac ttctcgcgtg acgggctcga gctgatggac
     3781 gtcgcgatcg ccgccggcgc ccgatcgcgg ctgtttcccg acgggcgcag cacgcggatc
     3841 ctcgtgctcg cgccgccgcc cgagcaggtc ccgcacatga tcatggcgca cgggatggcc
     3901 cacctgatcc gggagtacgg gctgcccggc agccggttcc tcgccggacc gcgcggcatc
     3961 gacttcgcgg cgctgtggtc ggagctcgac ggctgccagt ggaagggcat cccggtgtcg
     4021 ctgctcgggt cgtcgttcgg cttcgtgcac ttcttcgact ggatggagcg cgagggctgc
     4081 cgcctggagc tgcccgcggg cagccgcctg ctcgacgccg gcggctacaa gggcaagagc
     4141 cgcgagatcg gccgcgaggc gttcgtgacc tgggccgcgc agctgtgcgg gctgcgcccg
     4201 gcgcgcgtga tcaacctgct cgggatgacc gagctcgcga gccagatcta cgaccggctc
     4261 accccgggcg ccgcggacgt cgcgcgcatg aagctgccgc cgcactgggt gcggaccacc
     4321 gtggtcgagc cgcggtgccc ggggcagcgc ggccccgagc cggtcggcga cggcgagatc
     4381 ggccttttgc gccacgtcga tctcgccaac atcgagcgcc cgatcgcgat ccagagcgag
     4441 gacgtcggcc gcaccgtgac ggccagcgtc gacggcgagc tgcgccgcgg cttcgagatc
     4501 ctcggccgcg ccaggggcgc cgagccgcgc ggctgctcgc tcaccgccga ggacatcgcg
     4561 ccgcactccg gcgaggccgc gcagagcgcg tgcgcatgag gaggcgccga tgatcgcacc
     4621 ccggacgcgc accgccaacg ccggcccgac ccacgtggcg tgctggctgc ctcccgcgct
     4681 gcgcgaggcg atcgatgcga tgcccggcgg cgcgacgccg taccgcaagc gctacgatca
     4741 cgatgaccag cacctcgagg tcgagttcga gcggctgtcg cccgacgcgc tcgaccaggt
     4801 cgtcgcgttc ctccgccggc gccggcgcga ggtgctggcc cggcgcaccg tgggcgagat
     4861 cgtcgacgtg ctggagcgcg ccgcggtccg ctggctcgat ccggcgtacc cgccgcgcgc
     4921 cgccgcggtc ctcgagatcg cgttcatcac cgggttctcg ccggagatgg tcggccacgc
     4981 gatcgacgag gaacaggcga gctcgcgcgg gccgcacctc accctggcgc tgcgcagcga
     5041 gctcggcgac cccgagttcc tcgatggctt ccgccccaac ccgcggaccg gcggcctgag
     5101 ccgcgcccac ggcccggggc tggtcggcgc gatcttcagc tcgaacatcc ccgccctgcc
     5161 ccacctcgag atcatgcgca gcttcctggt gaagtcggcg tgcctgggac gggtgtcggc
     5221 cggcgaaccg atcttcctgc gccgctacgc cgagaccctg cacgagctcg atcccgagct
     5281 cgcgtcgtgc ctggcgatcg tctactggga gcgcggcgac gacgagctcg aggcgcggtt
     5341 cctgcgctcg atcgactacc tggtcgcgta cggcggcgac gcgcagatcc agcgcctgat
     5401 ggcgagccgg ccgggcgcgc tcgacgccac ctggcacggc catcggctgg gcttcaccta
     5461 cgtgacccgc gaggcgctcg ccgccggctc cgacctccgc cggctcgccc gcgccatcgc
     5521 ctacgacttc gcgatcttcg acggcttcgc gtgcctgtgc cc
//
LOCUS       DASVOR010001756         3768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_47599,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001756 DASVOR010000000
VERSION     DASVOR010001756.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47599"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1631)
                     /locus_tag="VF516_32695"
     CDS             complement(<1..1631)
                     /locus_tag="VF516_32695"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8112550.1"
                     /translation="MDEAKVRDAIELLEQLGTDLRALAEIDEPLRIRLVTAAGQLSRP
                     DRYSRKALGKVLARQRVQARRDADRARLDQTGIRLLRKEPVFRTPLPRPAMALDAPVR
                     DDALPDGWAAAEARRADPAESIDGDADAASRVEAARLCYVCKTEFHELHHFYDQMCPP
                     CAALNFDKRQQTADLRGRVALVTGGRVKIGYQAAIKLLRAGAEVIVATRFPRDAARRF
                     AREPDAADWTARLHVYGIDLRHTPSVERWCEHLLATLPRLDALINNACQTVRRPAGWY
                     DHVAAGELDDGRQLPAAERALLERNQALGHGIVRAGQIDERSESGGGDPAGSAGGAGG
                     EAPRGRDALRAAGLWKSAVLAQVPLLAEDLERGGHLYPAGRLDADLQQVDLRDFNSWR
                     MTLAEVPTAELLEVHLVNAVAPFVLNARLRPLLARVPTRDAHVVNVSAMEGQFYRRWK
                     TDKHPHTNMAKAALNMMTRTSAADYVRDGIHMNSVDTGWVTDEDPEVHATRKTRVHGF
                     HPPLDIVDGAARIVDPVFDGVNTGNHVWGVFLKDY"
     gene            1655..3043
                     /locus_tag="VF516_32700"
     CDS             1655..3043
                     /locus_tag="VF516_32700"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8112551.1"
                     /translation="MIIAKRLKGKAIATWLGPTGDAARRALTRARQRITNAPSRLDFY
                     FDIADPMSYLTAQAVSRLVAAYPVELAVHVITPPASDVDPAPGLRPRHAVRDARLIAE
                     YWNVDFPAHREADGALVREVGTALIRDRPAADQLRAAIELAGAMWRGDKKRLGELLLA
                     HGTESSSSVAPILNTNYAELRKAGHYQGAMLAYDGTWYWGIDRLPYLEAALAEATGVA
                     AAPVDPTGGSAPRPAGRAVGVPTPRLPPVVAPRPEAERGPLKLSDPPLVCELWFSFRS
                     PYSYLALERIEAVLAPYRVPLVLKPVAPMVARGMPLPNVKRMYIVRDAKREADRHGIP
                     FGELCDPLGPGIDHCIAIAHRAAQHGQAMAFARSAMRGIWAEALDMTEYVDLRRVVER
                     AGMAWDDARTALTPEAAGEAVKAAQANATELAVYSLWGVPSMRCGEFVAWGQDRLDLL
                     ADRLRRHALATR"
     gene            3138..>3768
                     /locus_tag="VF516_32705"
     CDS             3138..>3768
                     /locus_tag="VF516_32705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112552.1"
                     /translation="MFASTEPRTQPPLPAPAAVVSLPSPGLPADHGLASLGLVMQLAG
                     RTTAALVALLASLAWIDGRLHPHTAWCGLVVALCIARSQLHRAAGRDLTYGRRSAEGT
                     AADPFDAMRTYVVFALCHAVAIGAIAAGVFDQPWPAAAGIGAALALWPCALAVISHLP
                     RVQPLRAGLPLGEDRGLEGASIVMTVLGTYGVLSTGGIVVMLGALSPHHL"
BASE COUNT          465 a         1432 c         1342 g          529 t
ORIGIN      
        1 ttgtagtcct tgaggaacac cccccagacg tgattgccgg tgtttacacc gtcgaacacc
       61 gggtcgacga tccgcgccgc gccgtcgacg atgtcgagcg gcgggtggaa accatgaacg
      121 cgagtcttgc gcgtcgcgtg cacctcggga tcttcatcgg tgacccaccc ggtatcgacg
      181 ctgttcatgt ggattccgtc acggacgtaa tccgccgcag aggtccgcgt catcatgttg
      241 agggcggcct tggccatgtt ggtgtgcggg tgcttgtcgg tcttccaccg acggtagaac
      301 tggccctcca tcgccgagac gttgaccaca tgggcgtcgc gggtcgggac ccgggcgagc
      361 agcggccgga gccgggcgtt cagcacgaac ggcgccacgg cgttgaccag gtggacctcg
      421 agcagctcgg cggtcggcac ctcggcgagc gtcatccgcc agctgttgaa atcgcgcaga
      481 tcgacctgct ggaggtcggc gtcgagccgg ccggccgggt acaggtggcc gccgcgctcg
      541 aggtcctcgg cgagcagcgg cacctgggcc agcaccgcgg acttccacag cccggcggcg
      601 cgcagtgcgt ctctcccacg gggtgcttcg ccccccgccc cgccggccga gccggcaggg
      661 tccccacccc ccgactcgct gcgctcgtcg atctggcccg cgcggacgat cccgtggccg
      721 agcgcctggt tgcgctccag cagggcccgc tcggctgccg gcagctgccg cccatcgtcg
      781 agctcgccgg cggcgacgtg atcgtaccag ccggccggtc ggcgcacggt ctggcacgcg
      841 ttgttgatca gcgcgtcgag ccgcggcagc gtcgccagca ggtgctcgca ccagcgctcg
      901 acgctgggcg tgtggcgcag gtcgatgccg tagacgtgca accgcgcggt ccagtcggcg
      961 gcgtcgggct cgcgggcgaa ccgccgggcg gcgtcgcgcg ggaaccgggt cgcgacgatg
     1021 acctcggcgc ccgcgcgcag cagcttgatc gccgcctggt agccgatctt gacccggccg
     1081 ccggtgacca gcgcgacccg gccgcgcagg tcggccgtct gctggcgctt gtcgaagttg
     1141 agcgccgcgc acggcgggca catctggtcg tagaagtgat ggagctcgtg gaactcggtc
     1201 ttgcagacgt agcacaggcg cgcggcctcg acccggctcg ccgcatccgc gtcaccgtcg
     1261 atcgattcgg ccgggtcggc ccgccgggcc tcggccgccg cccacccgtc gggcagggcg
     1321 tcgtcgcgca ccggcgcatc gagcgccatc gccgggcgcg gcagcggggt ccggaagacc
     1381 ggctccttgc gcagcaggcg gatcccggtc tgatcgagcc gggcgcggtc ggcgtcgcgc
     1441 cgggcctgga cgcgctggcg ggcgagcacc ttgcccagcg ccttgcggct gtagcggtcg
     1501 ggccgcgaca gctggcccgc cgcggtgacc agccggatcc gcagcggctc gtcgatctcg
     1561 gcgagcgccc gcaggtcagt gcccagctgc tccagcagct cgatcgcatc acggaccttg
     1621 gcttcgtcca cggcggcgta tattgacgcg cggcatgatc atcgccaagc gcctcaaggg
     1681 caaagccatc gcgacctggc tcggaccgac cggcgacgcg gcacgccgcg cgctgacccg
     1741 ggctcgccag cggatcacca acgccccgag ccggctcgac ttctacttcg acatcgccga
     1801 tccgatgagc tacctcaccg cccaggcggt gtcgcggctg gtcgcggcct accccgtcga
     1861 gctcgccgtg cacgtcatca cgccgccggc gtccgacgtc gaccccgcgc ccggcctgcg
     1921 gcccaggcac gcggtgcgcg acgcccggct gatcgccgag tactggaacg tcgacttccc
     1981 ggcccaccgc gaggccgacg gcgccctggt ccgcgaggtc ggcaccgcgc tgatccgcga
     2041 tcgcccggcg gccgaccagc tgcgcgcggc gatcgagctc gccggcgcga tgtggcgcgg
     2101 cgacaagaag cggctcggcg agctcttgct cgcgcacggc accgagagct cgagctcggt
     2161 cgcgccgatc ctcaacacga actacgccga gctgcgcaag gccggccact accagggcgc
     2221 gatgctggcc tacgacggca cctggtactg gggcatcgat cgcctgccct acctggaggc
     2281 cgcgctcgcc gaggccaccg gcgtcgccgc cgcgccggtc gatcccacgg ggggctctgc
     2341 cccccgcccc gccggcagag ccgtcggggt ccccaccccc cgcctccctc cggtcgtcgc
     2401 cccgcgcccc gaggccgagc gcggtccgct caagctgtcc gaccccccgc tggtctgcga
     2461 gctgtggttc tcgttccgct cgccgtacag ctacctcgcg ctcgagcgca tcgaggccgt
     2521 gctcgcgccg taccgcgtgc cgctggtgct caagccggtc gccccgatgg tcgcgcgcgg
     2581 catgccgctg cccaacgtca agcggatgta catcgtgcgc gacgccaagc gcgaggccga
     2641 tcgccacggc atcccgttcg gcgagctgtg cgatccgctg ggcccgggca tcgaccactg
     2701 catcgcgatc gcccaccgtg ccgcacagca cgggcaggcg atggcgttcg cgcgctcggc
     2761 gatgcgcggg atctgggccg aggcgctcga catgaccgag tacgtcgacc tgcgccgcgt
     2821 cgtggagcgc gccgggatgg cgtgggacga cgcgcgcacc gcgctcaccc ccgaggcggc
     2881 cggcgaggcc gtgaaggccg cgcaggccaa cgcgaccgag ctcgcggtct acagcctgtg
     2941 gggcgtgccg tcgatgcggt gcggcgagtt cgtggcctgg ggccaggatc gcctcgacct
     3001 gctcgccgac cggctgcgcc gccacgcgct ggcgacgcgg tgacgtgaac catgaacaaa
     3061 cgtttcgccg cgcggccagg cccgcgatcg gccggctgtg cgccgcgcga cgatcggctg
     3121 catgctatgg atcaagcatg ttcgcgtcga ccgaaccgcg gacccagcca ccgctccctg
     3181 ctccggccgc ggtcgtgagc ctgccgagcc ccggcctgcc ggcggaccac gggctcgcca
     3241 gcctcggcct ggtgatgcag ctggccggcc gcaccaccgc ggcgctcgtc gcgctgctcg
     3301 cctcgctcgc gtggatcgac gggcgactcc acccccacac cgcgtggtgc gggctggtgg
     3361 tcgcgctgtg catcgcgcgc tcgcagctcc accgcgccgc cggccgcgat ctgacctacg
     3421 gccggcgcag cgccgagggc accgccgcgg atccgttcga tgcgatgcgc acctacgtcg
     3481 tgttcgcgct gtgccacgcg gtcgcgatcg gcgcgatcgc cgccggggtg ttcgatcagc
     3541 cgtggcccgc cgccgccggg atcggcgcgg cgctcgcgct gtggccgtgc gcgctcgcgg
     3601 tcatctccca cctcccccgg gtccagccgc tgcgcgcggg gctgccgctc ggcgaggacc
     3661 gcggcctgga gggcgcgtcg atcgtcatga ccgtgctcgg cacgtacggc gtgctgtcga
     3721 ccggcgggat cgtcgtgatg ctcggcgcgc tgtcgccgca ccacctgc
//
LOCUS       DASVOR010001757         6001 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_222704,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001757 DASVOR010000000
VERSION     DASVOR010001757.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6001
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222704"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VF516_32710"
     CDS             complement(<1..305)
                     /locus_tag="VF516_32710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112553.1"
                     /translation="MARRNPRPRPPRVVRLGAVAVAVALGATVVVALGGAIVPPVAHA
                     QPAPDLERARELYRSAEAAMQDGRYEDAADKEAAERDGKAREERQAREAAEKEAKAK"
     gene            350..2410
                     /gene="fusA"
                     /locus_tag="VF516_32715"
     CDS             350..2410
                     /gene="fusA"
                     /locus_tag="VF516_32715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEX8112554.1"
                     /translation="MAKIDLVRNLGVVAHIDAGKTTVSERFLFHSGKIHKVGEVHDGE
                     TEMDWMEQERERGITITAAATTFEWKKHEIHLIDTPGHVDFTIEVERSLRVLDGAVVV
                     FCGVAGVQPQSETVWHQADKFKVPRLAFVNKLDRIGASFPAVVADIRERLGANAVPIQ
                     LPIGSEDAFSGAIDLVRMKAIMFSGDVTDTPEVVDIPGELAAVAADARDAMIQSLADL
                     DDGIAVKYLEGQDLPAAELDAAIRKATIAVKMVPVLGGTALRNKGIHPLLDAVIAYLP
                     SPADVPPVTGVDPRTIHAENGPDKLTRAPRNNEPFSALAFKIAMDEGRKVVFLRIFSG
                     VLEAGAEILNARTNRKERVARLFRIHANKRERLDKAVAGEIVAAAGLKDATTGDTVCD
                     PKAPILLERIDTYEPVISQAIEADNAAAKERLDFALAKMSDEDPTFRFREDADTGQTL
                     ISGMGELHLEIIVDRMKREYGVQARAGKPQVVYRETVLGEGEGHAVFERSISTSGKEQ
                     SIYGEATCKVRGRPRGSGMDVKRALPAGALLPDGIIQAAMQGLRDAASSGPDGFPLED
                     VEVTLLGLVLRDGANAEIGSRAAAAEAFRHAVQAATPSRLEPIMAVEVTVDDEFLGAV
                     IGDLNQRRGQVQDVGSRGAKRVVAARVALRNMFGYSTRLRSLTEGRATFTMMFHAYDT
                     LQAA"
     gene            complement(2419..3717)
                     /locus_tag="VF516_32720"
     CDS             complement(2419..3717)
                     /locus_tag="VF516_32720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112555.1"
                     /translation="MPTASIARCALVAVAAAGAAGCFSDRGLAIQIDVAGTGATSVEL
                     FLGKTACDASDNPAEIDCKAIAPPPASSAPLRGSIWFRDDLAVDTVQVTGPRVTFQLR
                     SDNAATLPIVIAVGLTQGDHPVGVATLHDVAIPTGSARVLATALAPAGAVQAAAPAKT
                     AVEDRVQVWRKTAPPSSCVVAEHWEGGAATRSFVVPGQDPDCDDFVAEDCAPAVYLGM
                     TAPGTASRPDCFTRQSGACVLGSRACTDGSANRTGECGPQHNAAICVPDPFCTCTGDI
                     GDLCWQQAVRTMPLAMPVIDCSVATTQIAPFTFCPGTEPDMATIDIGGHFAQDETCGT
                     PTIASSSLLGFGSSAPFGGALMELSSPRRPCNFSITWKSGARTSGDAVDVGAVKLQTA
                     TGALVLPIVLRFHPTDCLGPQFTCTLMDVATDSVWSCAAP"
     gene            complement(3825..4955)
                     /locus_tag="VF516_32725"
     CDS             complement(3825..4955)
                     /locus_tag="VF516_32725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112556.1"
                     /translation="MIALVLTASAGAALAQPRPMPPQPVWDSRGWVMLGERTVNGRAD
                     HDRIEVGRQDGRFNKLTMVVENSNLELIDFRIVFGDRTEYHPKLSYVFREGQRTRAID
                     LPPSESVIRFIDIQYRNLSAGEARVQIWGFRTGNAMPPPPPPPPPAPAPAPAWDPKGW
                     TLLGERTVDGRFDHDRIDVGRYEGRFSKLTIVVENSDLELIDLSIKFEHGPPWHPAVQ
                     HFFREGQRTRAIDFPGDERIIKHIDFRYRNLPGGGRARVAVYGLREAPVWESRGWTML
                     GERTVDGSREDYDRIEVPRSEGRFRKLTVVVLDGDLEMIDMLVELGHGEPFHPEINAF
                     FRENTRTRVIDLPGEARRIRGIQFRYRNLRGGARARVQVWAR"
     gene            5573..>6001
                     /locus_tag="VF516_32730"
     CDS             5573..>6001
                     /locus_tag="VF516_32730"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: protein motif:HMM:TIGR00571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HEX8112557.1"
                     /translation="MSVRSLSRAKRDLRPALRDSPAGPVVKWVGGKTKLLPELLARMP
                     ARFGRYYEPFAGGAALFFRVAPERAVLADSNSDLIGLYTCLADDVAGVVRRLEHHRAA
                     HCEAHYYATRTRWNDREASWSSADRAATFIYLNKTCFNGLW"
BASE COUNT          868 a         2104 c         2192 g          837 t
ORIGIN      
        1 tccttcgcct tggcttcctt ctcggctgct tcgcgggcct gccgctcctc gcgtgccttg
       61 ccatcgcgtt cggcggcttc cttgtcggcg gcgtcctcgt agcggccgtc ctgcatcgcg
      121 gcctcggccg agcggtacag ctcccgggcg cgctccaggt cgggggccgg ctgggcgtgg
      181 gcgaccgggg ggacgatcgc gccgccgagg gcaacaacca ccgtggcacc gagggcgacc
      241 gcgaccgcca ccgcgcccag ccgcaccacg cgcggcgggc gaggccgagg attgcgccgg
      301 gccatgcagc gagcgtaccc caactgcgct ataccgcccg ccggacgtca tggcgaagat
      361 cgatctcgtt cgcaacctcg gtgtggtggc tcacatcgat gccggcaaga ccacggtgtc
      421 ggagcggttc ctgttccact ccggcaagat ccacaaggtc ggcgaggtcc acgacggcga
      481 gaccgagatg gactggatgg agcaggagcg cgagcgcggg atcacgatca cggccgcggc
      541 gaccacgttc gagtggaaga agcacgagat ccacctgatc gacacgccgg gccacgtcga
      601 cttcacgatc gaggtcgagc gctcgctgcg cgtgctcgac ggcgcggtcg tggtgttctg
      661 cggcgtggcc ggcgtccagc cgcagtccga gacggtgtgg caccaggccg acaagttcaa
      721 ggtgccgcgg ctggcgttcg tcaacaagct cgaccggatc ggcgcctcgt tcccggcggt
      781 ggtcgccgac atccgcgagc ggctgggcgc gaacgccgtg ccgatccagc tgccgatcgg
      841 cagcgaggac gcgttctccg gcgcgatcga cctggtgcgg atgaaggcga tcatgttcag
      901 cggcgacgtc accgacacgc cggaggtggt cgacatcccg ggcgagctcg ccgcggtcgc
      961 cgccgacgcg cgcgacgcga tgatccagtc gctggccgac ctcgacgatg ggatcgcggt
     1021 caagtacctg gagggccagg acctgcccgc ggccgagctc gacgccgcga tccgcaaggc
     1081 gacgatcgcg gtcaagatgg tgccggtgct cggcggcacc gcgctgcgca acaagggcat
     1141 ccacccgctg ctcgacgcgg tgatcgccta cctgccgtcg ccggccgacg tgccgccggt
     1201 gaccggggtc gatccgcgga cgatccacgc cgagaacggc cccgacaagc tgacccgcgc
     1261 gccgcgcaac aacgagccgt tctcggcgct ggcgttcaag atcgcgatgg acgagggccg
     1321 caaggtcgtg ttcctgcgga tcttctcggg cgtgctggag gccggcgccg agatcctgaa
     1381 cgcgcgcacc aaccgcaagg agcgggtcgc ccggctgttc cggatccacg ccaacaagcg
     1441 cgagcggctc gacaaggcgg tcgccggcga gatcgtcgcg gccgccgggc tcaaggacgc
     1501 caccaccggc gacacggtgt gcgatcccaa ggcgccgatc ctgctcgagc ggatcgacac
     1561 ctacgagccg gtgatctcgc aggcgatcga ggccgacaac gcggcggcca aggagcggct
     1621 cgacttcgcg ctggccaaga tgtccgacga ggatccgacg ttccggttcc gcgaggacgc
     1681 cgacaccggc cagaccctga tcagcgggat gggcgagctc cacctcgaga tcatcgtcga
     1741 ccggatgaag cgcgagtacg gcgtgcaggc gcgcgccggc aagccgcagg tcgtgtaccg
     1801 cgagaccgtg ctcggcgagg gcgagggcca cgcggtgttc gagcggtcga tctcgacctc
     1861 cggcaaggag cagtcgatct acggcgaggc gacgtgcaag gtgcgcggcc ggccgcgcgg
     1921 cagcggcatg gacgtcaagc gcgcgctgcc ggccggagcg ctgctgcccg acgggatcat
     1981 ccaggccgcg atgcagggcc tgcgcgacgc ggcatcgagc ggccccgacg ggtttccgct
     2041 cgaggacgtc gaggtcacgc tgctcggcct ggtgctgcgc gacggcgcca acgccgagat
     2101 cggcagccgc gccgccgcgg ccgaggcgtt ccgccacgcg gtccaggcgg ccaccccgtc
     2161 acggctcgag ccgatcatgg cggtcgaggt cacggtggac gacgagttcc tcggcgcggt
     2221 gatcggcgat ctcaaccagc ggcgcggcca ggtccaggac gtcggctcgc gcggcgccaa
     2281 gcgggtggtc gcggcgcgcg tcgcgctgcg caacatgttc ggctactcga cccggctgcg
     2341 cagcctgacc gagggccgcg cgacgttcac gatgatgttc cacgcctacg acacgctgca
     2401 ggccgcgtag ccgggctgct aaggcgccgc gcagctccac accgagtcgg tcgcgacgtc
     2461 catcagggtg caggtgaact gcggacccag gcagtcggtg gggtggaagc gcaggacgat
     2521 cggcaggacg agcgcgccgg tcgcggtctg gagcttgacc gcgccgacgt cgacggcatc
     2581 gcccgacgtg cgggccccgg acttccaggt gatcgagaag ttgcacggcc tccgcgggct
     2641 cgacagctcc atcagcgcgc cgccgaacgg cgcgctcgaa ccaaagccga gcagcgagct
     2701 cgacgcgatc gtcggcgtgc cgcaggtctc gtcctgcgca aagtggcccc cgatatcgat
     2761 cgtggccatg tccggctcgg tccccgggca gaacgtgaag ggcgcgatct gggtcgtggc
     2821 gaccgagcaa tcgatgaccg gcatcgccag gggcatggtc cggaccgcct gctgccagca
     2881 caggtcgccg atgtcccccg tgcaggtgca gaacgggtcg gggacgcaga tcgcggcgtt
     2941 gtgctgcggc ccgcactcgc cggtgcggtt cgcgctgccg tcggtgcagg cgcggctgcc
     3001 cagcacgcag gccccggact gccgggtgaa gcaatccggc cgcgacgccg tgcccggggc
     3061 cgtcatgccg aggtacacgg cgggggcgca gtcctcggcg acgaaatcat cgcagtccgg
     3121 atcctggccc ggcaccacga agctgcgggt cgccgcgccg ccttcccagt gctcggcgac
     3181 cacgcacgag ctcggcggcg cggtcttgcg ccagacctgg acgcgatcct cgacggcggt
     3241 cttggccggg gccgccgcct gcaccgcgcc ggcgggcgcc agcgcggtcg cgagcacccg
     3301 cgcgctgccg gtcgggatcg cgacgtcgtg cagcgtcgcg acgccgaccg ggtgatcgcc
     3361 ctgggtgagg ccgaccgcga tcacgatcgg gagggtggcg gcgttatcgg accggagctg
     3421 gaacgtcacc cgggggccgg tgacctggac cgtgtcgacc gcgagatcgt cgcggaacca
     3481 gatcgagccg cgcagcggcg ccgagctggc cggcggcggc gcgatcgcct tgcagtcgat
     3541 ctccgcgggg ttgtcgctcg catcgcacgc ggtcttgccg aggaacagct cgaccgaggt
     3601 cgcgccggtg cccgcgacgt cgatctggat cgcgaggccg cgatccgaga agcacccggc
     3661 cgcccccgcg gcggcgacgg ccactagcgc gcaccgcgcg atcgaggcgg ttggcatcgc
     3721 gaccaggata ccgccggccg tcgggcggtt ctagccgccg cccgtcgcag cgtgatctgg
     3781 cccgggtgtg cagccagcgt gcacaccggc gtgcgcgccg cgcgctaccg ggcccacacc
     3841 tggacgcggg cgcgggcgcc accacgcaag ttgcggtagc ggaactggat cccgcggatc
     3901 cggcgcgcct cgcccggaag atcgatcaca cgggtccggg tgttctcgcg gaagaacgcg
     3961 ttgatctcgg ggtggaacgg ctcgccgtgg ccgagctcga ccagcatgtc gatcatctcg
     4021 aggtcgccgt cgagcacgac cacggtcagc ttgcggaacc ggccctcgct gcgcggcacc
     4081 tcgatgcggt cgtagtcctc gcggcttccg tcgaccgtgc gctcgccgag catcgtccag
     4141 ccccgcgact cccagaccgg cgcctcgcgc aggccgtaga ccgcgacccg ggcgcggccg
     4201 ccgccgggca ggttgcggta ccggaagtcg atgtgcttga tgatccgctc gtcgccgggg
     4261 aagtcgatcg cccgggtgcg ctggccctcg cggaagaagt gctgcaccgc ggggtgccac
     4321 ggcgggccgt gctcgaactt gatcgacagg tcgatcagct cgaggtcgct gttctcgacc
     4381 acgatggtga gcttgctgaa ccgcccctcg tagcggccga cgtcgatccg gtcgtggtcg
     4441 aaccggccgt cgaccgtgcg ctcgccgagc agggtccagc ccttcggatc ccacgcgggt
     4501 gcgggtgcgg gtgcgggcgg gggcgggggc gggggcgggg gcatggcgtt gccggtgcgg
     4561 aagccccaga tctggacgcg ggcctcgccg gccgacaggt tgcggtactg gatgtcgatg
     4621 aaccggatca ccgactcgct cggcggcagg tcgatcgccc gcgtgcgctg gccctcgcgg
     4681 aacacgtagc tgagcttggg gtgatactcg gtgcgatcgc cgaacacgat ccggaagtcg
     4741 atcagctcga ggttgctgtt ctcgaccacc atggtgagct tgttgaaccg gccgtcctgg
     4801 cggccgacct cgatccggtc gtgatcggcc cggccgttga cggtgcgctc gccgagcatg
     4861 acccagccgc gcgagtccca cacgggctgg ggcggcatcg gtcggggctg ggcgagcgcg
     4921 gcaccggcgc tcgcggtcag gaccagggct atcacgggaa gcattcgacg catggcacgg
     4981 aaactaacac agggtcgcat cggccctcct gcgggatcga cggtcggcat cgactgtgac
     5041 gtcggtcggg gtaaaaaatt gaaggcgaat tttttgcgcg gaggatcgtc tacgctcggc
     5101 cggcgtgagg tcgcgccagg ctgtgatccg ggttcgggtg ccagcgccgg cgcggccgcg
     5161 agctcctgct cgggccagaa ctcgcttggc cccggccgcg ctcgctcgct cgatcgatga
     5221 ccgaccggtc gggtccggcg tccgcgcgcc gtccacgccg gaggaccgcg cgccggaccg
     5281 atcgcgatcc gggacgctcg cggatcgatc gcggttcggg ctgctgcgag ccgatcgttc
     5341 gggcgccgcg cctgcgaacg agctcggcca cgatcaggcc ggcggcactc gccgacgccc
     5401 cggtctcgcc ggtgatcgtg cgctcggccc ggccgatgtg tccgccgccc ggacgccggg
     5461 cgctcgctta tcgaactgga tcacgaagcg cgagcctgca gctcacaagg atcgtgccac
     5521 tgccagcgcc aaaaaattca ccccgatcgc gcgacctccg acgatcgccg acatgtctgt
     5581 tcgctcgttg tcgcgcgcga agcgcgatct gcgcccggcg ctccgggatt cgccggcggg
     5641 tccggtcgtc aagtgggtcg gtggcaagac caagctcctg cccgagctgc tcgcccggat
     5701 gccggcgcgg ttcgggcgct attacgagcc gttcgccggc ggcgccgcgc tgttcttccg
     5761 cgtcgcgccc gagcgcgccg tgctcgccga ctccaactcc gacctgatcg ggctctacac
     5821 ctgcctggcg gacgacgtcg ccggggtggt ccgccggctc gagcaccacc gcgcggcgca
     5881 ctgcgaggcg cactactacg cgacccgcac gaggtggaac gatcgcgagg cgtcgtggag
     5941 ctcggccgat cgcgccgcca cgttcatcta cctcaacaag acctgcttca acggcctgtg
     6001 g
//
LOCUS       DASVOR010001758         5988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_310665,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001758 DASVOR010000000
VERSION     DASVOR010001758.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5988
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310665"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="VF516_32735"
     CDS             complement(<1..216)
                     /locus_tag="VF516_32735"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump"
                     /protein_id="HEX8112558.1"
                     /translation="MWSAIALAFGLAMDATAVSAARGLAGRRPRELVLLPALFGGFQS
                     AMSALGWLAGRWAGPYIERWDHWVAFGL"
     gene            complement(233..1393)
                     /locus_tag="VF516_32740"
     CDS             complement(233..1393)
                     /locus_tag="VF516_32740"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HEX8112559.1"
                     /translation="MEIVIAEAVRSAVGRGHKGSLATKRPDELAADVIRGLLARVPQA
                     RGKIDDVVLGCAMPEGEQGLNVARLIALSADLGIEVPAQTINRFCSSGLQAIAIAAGS
                     IAVGSNDVVLAGGVESMTMVPMTGFHLSASPELMARLPGANTPMGITAENVARRFGIT
                     RQMQDEFAYRSQVKARRALETGAFKDEIVPVRAVGYEGDHRVYRDFTVDEMPRPDTTL
                     EGLAKLPPAFAQGGSVTAGNSSPISDGAAAALVMSRQAADRLGVTPLGIFRAFVTVGV
                     DPEIMGIGPLPAVQKLLAKTGLKVADIDVFELNEAFASQSVYCQRELGIPDDKINVHG
                     GAIALGHPLGCTGAKLTATALYELKRRGGRYAVVTMCIGGGQGAAGLFERTA"
     gene            complement(1421..3838)
                     /locus_tag="VF516_32745"
     CDS             complement(1421..3838)
                     /locus_tag="VF516_32745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HEX8112560.1"
                     /translation="MALATRIPPIRRVAVLGAGVMGSGIAAHCANAGIPVVLLDIVPP
                     KLGDAERQSRAKRDGLAAGALDKLKKARPAALMHPRNAQLISIGNFDDDLARVADADL
                     IVEAIVERLDVKQALFEKLEQLAAPHAVIASNTSGLRIADMVAGRGEAFRQRFLIAHF
                     FNPPRYMKLLELVAGVDTSPEARARVERFGREQLGKGIVWAKDTPNFIGNRIGLQSMM
                     TTIHLMLERGLAPEDVDAITGVAMGHPKSATFRTADVVGLDTVGHVAANCYQSLTGDE
                     ARATFKLPDYITKMIENGQLGDKTRGGFYKKQGDALLTLDPKTGEYRPKAGDPDIAKA
                     TKAVARIDDVRERVKALVAAPGAAGAFAWTVLSRSLAYAARRIPEIADSPDAVDDAMK
                     WGYAWDLGPFETWDALGFAETCARMKQDGVALPAWIDRMVAAGAGGFYEDTRVWNPQR
                     GEYVERALDPREETFEDMRHAAGRPRGSKAPTHGPVLKNAGAEAWDLGDGVLGLTFKT
                     KANSIDADVIKTIYDAVDRAEREFRAMVVWNQGEFFCVGANLFAVVMAAGQKQWDALR
                     ESARSYQYATQRLKYAMVPVVAAPYNMTLGGGLELCFGCAEVQAAAETYSGLVEVGVG
                     LIPGGGGTMNMLWRALGSVPEGVELDTYAAVTQVFKNIAMAKVATSAEEGKAFGYFAP
                     ADGVSFDRARQLWETKQRAIGLASAGYHPPVPRAYKLPGDSGIATLHMMVNTLVAGGY
                     ASAHDAKIAMKLATVLCGGASGGSRAVTEDEMLELEREAFVSLCGEPLSQARMQYMLQ
                     HNKPLRN"
     gene            4233..5291
                     /locus_tag="VF516_32750"
     CDS             4233..5291
                     /locus_tag="VF516_32750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112561.1"
                     /translation="MENITRVFMSDLHMCSSDAVAPAGGRRAYGWLSEPRARRTAEFL
                     ASNTVTDCQQLLLVGDVIDLWICPHDVRPPSARDVLDAPHNKPVVEAIRAFARLPGRS
                     VIWCQGNHDAEATAETARYLADGAQLVPRHNVDFPLRAQHGHEDCLFNGPDPMDRPFP
                     LGYFISRFVATAAARGLAEVGVNIRTFLKVGPELAILLETEPLAQIVFEVVRKAADLE
                     LEDPVILPDGRTITVGAVRKEYENLVAEWRRNRSISVARALLCESDPYHDVPTGLHLN
                     IAGHSHEAKVKPELRYINLGSWCGQDAYFGKTWLVHAERLNETLCGALYKWDNGVVTQ
                     VGETVAVPTLWVPATKAA"
     gene            5571..>5988
                     /locus_tag="VF516_32755"
     CDS             5571..>5988
                     /locus_tag="VF516_32755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112562.1"
                     /translation="MRDPAAAAIAEPRSLAPALSGYKPSNLKKGKAALVGLNEAQQIR
                     SESYRNARGATVDAGGFSIWRGDPVGGFAAGGVPPTIWPTMEVLAVDRARAAVRDSVV
                     RDVAALGSTFGYLPEPQRELAAPAPPAGSKRQGGERQ"
BASE COUNT          836 a         2164 c         2090 g          898 t
ORIGIN      
        1 caggccgaac gcgacccagt ggtcccagcg ctcgatatag ggaccggccc accgtccggc
       61 gagccagccc agcgcggaca tcgcggactg gaagccgccg aacagcgccg gcagcagcac
      121 cagctcgcgc ggccgccgcc cggcgagccc gcgcgcggcc gacaccgccg tcgcatccat
      181 cgcgagcccg aacgcgagcg cgatcgccga ccacatcgac tgcgcgccgg ccctatgcgg
      241 ttcgttcgaa cagcccggcc gcgccctggc cgccaccgat gcacatcgtg accaccgcat
      301 agcgcccgcc gcgccgcttg agctcgtaca gcgcggtggc ggtcagcttg gcgcccgtgc
      361 agcccagcgg gtggccgagc gcgatcgcgc cgccgtgcac gttgatcttg tcgtcgggga
      421 tgccgagctc gcgctggcag tacaccgact ggctggcgaa cgcctcgttg agctcgaaca
      481 catcgatgtc ggcgaccttc aatccggtct tcgcgagcag cttctggacc gccggcagcg
      541 gcccgatccc catgatctcg ggatcgacgc cgacggtcac gaacgcgcgg aagatgccga
      601 gcggcgtgac cccgagccgg tcggcggcct ggcgcgacat caccagcgcc gccgccgcgc
      661 cgtcggagat cggcgacgag ttgccggccg tgacgctgcc gccctgggcg aacgccggcg
      721 gcagcttggc caggccctcg agcgtggtgt cgggccgcgg catctcgtcg accgtgaagt
      781 cgcggtacac gcggtggtcg ccctcgtagc cgaccgcgcg caccgggacg atctcgtcct
      841 tgaacgcgcc ggtctccagc gcgcgcctgg ccttcacctg cgagcggtac gcgaactcgt
      901 cctgcatctg ccgcgtgatc ccgaaccgcc tggcgacgtt ctcggcggtg atccccatcg
      961 gcgtgttggc gcccggcagc ctggccatca gctcgggcga cgccgacagg tggaagccgg
     1021 tcatcggcac catggtcatc gactcgacgc cgcccgcgag cacgacgtcg ttggacccga
     1081 ccgcgatcga gccggccgcg atcgcgatcg cctgcaggcc gctcgagcag aaccggttga
     1141 tggtctgggc cgggacctcg atcccgaggt cggcggacag cgcgatcagc cgcgcgacgt
     1201 tgagcccctg ctcgccctcg ggcatcgcgc agcccagcac gacgtcgtcg atcttgcccc
     1261 gggcctgggg cacgcgggcc aggagcccgc ggatcacgtc ggcggcgagc tcgtcggggc
     1321 gcttggtggc gaggctaccc ttgtgaccgc ggccgaccgc ggagcgaact gcttcggcaa
     1381 tgacgatttc catggcgatt cctcgtgggt cggcgggttt tcagttccgc agtggcttgt
     1441 tgtgctgcag catgtactgc atgcgggcct gcgacagcgg ctcgccgcac aggctgacga
     1501 acgcctcgcg ctcgagctcc agcatctcgt cctcggtcac ggcgcggctt ccgcccgacg
     1561 cgccgccgca cagcacggtg gcgagcttca tcgcgatctt ggcgtcgtgc gcgctggcgt
     1621 agccgccggc caccagcgtg ttgaccatca tgtgcagggt cgcgatcccg ctgtcgccgg
     1681 gcagcttgta ggcgcgcggc accggcgggt ggtagccggc gctggccagc ccgatcgcgc
     1741 gctgcttggt ctcccacagc tggcgcgcgc ggtcgaacga cacgccgtcg gccggcgcga
     1801 agtagccgaa cgccttgccc tcctcggcgc tggtcgcgac cttggccatc gcgatgttct
     1861 tgaacacctg ggtcaccgcg gcgtaggtgt cgagctcgac gccctcgggc acgctgccca
     1921 gcgcgcgcca cagcatgttc atcgtcccgc cgccgcccgg gatcagcccg acgccgacct
     1981 cgaccaggcc ggagtaggtc tcggccgcgg cctggacctc ggcgcagccg aagcacagct
     2041 cgagcccgcc gccgagcgtc atgttgtagg gcgcggcgac caccggcacc atcgcgtact
     2101 tcaggcgctg ggtcgcgtac tggtagctcc gggccgactc gcgcagggcg tcccactgct
     2161 tctgcccggc ggccatcacc accgcgaaca ggttggcgcc gacgcagaag aactcgccct
     2221 ggttccacac caccatcgcg cggaactcgc gctcggcgcg gtcgaccgcg tcgtagatcg
     2281 tcttgatcac gtcggcgtcg atcgagttcg ccttggtctt gaacgtcagg ccgagcaccc
     2341 cgtcgccgag gtcccacgcc tcggcgcccg cgttcttgag caccgggccg tgggtcgggg
     2401 ccttcgatcc gcgcggccgg cccgccgcgt ggcgcatgtc ctcgaacgtc tcctcgcgcg
     2461 gatcgagcgc gcgctcgacg tactcgccgc gctgcgggtt ccacacccgc gtgtcctcgt
     2521 agaacccgcc ggcgccggcg gcgaccatcc ggtcgatcca cgccggcagc gcgacgccgt
     2581 cctgcttcat ccgcgcgcag gtctcggcga acccgagcgc gtcccaggtc tcgaacggcc
     2641 cgaggtccca cgcgtagccc cacttcatcg cgtcgtcgac cgcgtccggc gagtcggcga
     2701 tctccgggat ccggcgcgcc gcgtacgcca ggctgcgcga cagcaccgtc caggcgaacg
     2761 cgccggcggc gcccggcgcc gcgaccagcg ccttgacccg ctcgcggacg tcgtcgatcc
     2821 gcgccaccgc cttggtcgcc ttggcgatgt ccggatcgcc ggccttgggc cggtactcgc
     2881 cggtcttcgg atcgagcgtc agcagcgcgt cgccctgctt cttgtagaac ccgccccggg
     2941 tcttgtcgcc gagctggccg ttctcgatca tcttcgtgat gtagtcgggc agcttgaacg
     3001 tcgcgcgcgc ctcgtcgccg gtcagcgact ggtagcagtt cgccgcgacg tggccgaccg
     3061 tgtcgagccc gaccacgtcc gcggtgcgga acgtcgcgct cttggggtgc cccatcgcga
     3121 ccccggtgat cgcgtcgacg tcctcgggcg caagcccgcg ctccagcatc aggtggatcg
     3181 tcgtcatcat cgactgcagg ccgatccggt tgccgatgaa gttcggcgtg tccttggccc
     3241 acacgatgcc cttgccgagc tgctcgcgcc cgaaccgctc gacccgcgcc ctggcctcgg
     3301 ggctggtgtc gactccggcg accagctcga gcagcttcat gtagcgcggc gggttgaaga
     3361 agtgcgcgat caggaagcgc tggcggaacg cctcgccgcg cccggcgacc atgtcggcga
     3421 tccgcaggcc cgacgtgttc gacgcgatca ccgcgtgcgg cgccgccagc tgctccagct
     3481 tctcgaacag cgcctgcttg acgtcgagcc gctcgacgat cgcctccacg atcaggtcgg
     3541 cgtcggcgac cctggcgagg tcgtcgtcga agttgccgat cgagatcagc tgtgcgttgc
     3601 gcgggtgcat cagcgccgcc ggcctggcct tcttcagctt gtcgagcgcg cccgccgcca
     3661 gcccgtcgcg cttggccctg gactgccgct cggcgtcgcc gagcttgggc gggacgatgt
     3721 cgagcaggac gaccgggatg ccggcgttgg cgcagtgggc ggcgatgccg gaacccatca
     3781 cgccggcgcc gagcaccgcc acgcggcgga tcggagggat gcgcgttgcg agagccatgc
     3841 ggccaccata gcgcagcgcg gcgaggatgg atcgcagcgg gccgcctcgc gcacgcgctg
     3901 tggcgattcg cccgcgtgct ccgaacccgt ctcatggcgg ccgagctggc ccgggcactc
     3961 ggcacatcga gcggagtggc cgagttgctc ccgatggtcg tcggtcttcc cgcccggcgg
     4021 cagcggcgcg tatcgctgcg gatcgccgcg tgcacgagat gcatcgcgac cgcggacttc
     4081 gtgcagcgcc tgcgcgctcg catgcgctcg acacacgggg ggtgctcgca gcgttgccgg
     4141 cgtgtcgagc actccgttcc gttcgcgatg gtgtggtgtt cagggcacat cgcatgcaag
     4201 gcctgggcag gtcggaacca gggaggaccg ccatggagaa catcacgcgc gtgttcatga
     4261 gtgacctgca catgtgttcg tcggatgcag tggcgccggc cggaggcagg cgcgcgtacg
     4321 gctggttgtc ggagcccagg gccagacgca cggccgagtt cctcgcgtcg aacaccgtga
     4381 ccgactgcca gcagctgctg ctggtcggtg acgtcatcga tctgtggatc tgtccgcacg
     4441 acgtccggcc gccgagcgcg cgggacgtgc tcgatgcgcc gcacaacaag cccgtggtcg
     4501 aggcgatccg ggcgttcgcc aggctgccgg gccggagcgt gatctggtgc cagggcaacc
     4561 acgacgcgga ggcgaccgcc gagaccgcgc gctacctggc ggacggcgcg cagctcgtgc
     4621 cgcgccacaa cgttgacttc ccgctccgcg cccagcacgg ccacgaggac tgcctgttca
     4681 acgggcccga tccgatggac cggccgttcc cgctcggcta cttcatctcg cggttcgtcg
     4741 cgaccgcggc ggcgcgcggg ctggccgagg tgggggtgaa catccggacg ttcctcaagg
     4801 tcgggcccga gctcgcgatc ctgctcgaga ccgagcccct cgcgcagatc gtgttcgagg
     4861 tcgtgcgcaa ggccgccgac ctcgagctcg aagatccggt gatcctgccg gacggccgca
     4921 cgatcacggt gggcgcggtg cgcaaggagt acgagaacct ggtcgcggag tggcggagga
     4981 accggagcat ctcggtggcg cgcgcgctgc tgtgcgagtc ggacccgtac catgacgtgc
     5041 ccacggggct gcacctgaac atcgccggcc attcgcacga agcgaaggtc aagccggagc
     5101 tccggtacat caacctcggc tcgtggtgcg gccaggatgc atacttcggc aagacgtggc
     5161 tcgtgcacgc cgaacggctc aacgagaccc tgtgtggcgc gctctacaag tgggacaacg
     5221 gcgtggtcac ccaggtcggc gagacggtcg cggttccgac gctgtgggtg cccgcgacca
     5281 aggccgccta ggtacacctg tcttccgcgc gccaggccga agaggtcgca ccgctccagg
     5341 cgagcaagcg gtgaccgatt ttccgctgca gccaccgctg ggaccgcagc atggtcgttg
     5401 ctggtgcgat gcgccaactg gccgaaaact ggtgctgacc cgcctggcgg gttcgtcttg
     5461 gtacacaggt cagacccgcg cgatacaacg gtaagacgac aggacatggc gaaaccccga
     5521 gcggcgctag aaccgcctcg cgatcgccgc ggccgactgt cgaccaggta gtgcgcgatc
     5581 cagccgccgc ggcgatcgcc gagccgcggt ccctcgcgcc tgctctcagc ggctacaagc
     5641 cgtccaacct gaagaagggg aaggcagcgc tggtcggcct caacgaggcg cagcagatcc
     5701 ggagcgagag ctaccgcaac gcgcgcggtg cgaccgtgga cgctggcggg ttctcgatct
     5761 ggcggggtga tccggtgggg ggattcgcgg cgggcggcgt gccccctacc atctggccca
     5821 cgatggaggt cctggcagtc gaccgggccc gcgccgcggt gcgtgactcc gtcgtgcgcg
     5881 acgtggcggc gctcggctcg accttcggct acctgcccga gccacagcgc gagctcgccg
     5941 cgccggcgcc gcccgccggc tcgaagcggc aaggcggtga gcggcagt
//
LOCUS       DASVOR010001759         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_164043,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001759 DASVOR010000000
VERSION     DASVOR010001759.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164043"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VF516_32760"
     CDS             <1..210
                     /locus_tag="VF516_32760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112563.1"
                     /translation="PSSPLGAAPWQSGQPLPPPAVDRVARSWRIIDLPAFDPAQFERQ
                     RRIVLRIALAVVGLVIALALFAGRC"
     gene            264..1397
                     /locus_tag="VF516_32765"
     CDS             264..1397
                     /locus_tag="VF516_32765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HEX8112564.1"
                     /translation="MPRSKVAVLRVRPEHVLEDIDRLHGLAEVDRALTAGATTILKDN
                     ISWHFPFPGANTTPWQLEGTVKSLRARGFTDLVCVQNKTVVTDAFKGEDLNGYVPIFR
                     HHGVPVRYNFKPEDMTWSVYRPRAKMRVLDTIFPEGIQIPDAFHGTNIVHLPTVKCHI
                     YTTTTGAMKNAFGGLLNTRRHYTHSWIHETLVDLLAIQKEIHAGLFAVMDGTTAGDGP
                     GPRTMRPVIKDVMLASADQVAIDAVAASMMGFDPLTIPYLRLADEAGLGNARREAIDI
                     AGDAALADQRWGFSVGDNGASLVGDVMWFGPLKRFQNLFFRTPLVNLFVLGSEAYHDY
                     YRWPLRDRRVFEHWRQTTHWGRLFDRYQAEGTLGAAAVATQAG"
     gene            complement(1415..>1617)
                     /locus_tag="VF516_32770"
     CDS             complement(1415..>1617)
                     /locus_tag="VF516_32770"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8112565.1"
                     /translation="ARLRVAIAQLPDRPRALVTKHYFEGKSLLEAGAELGVSKSWASR
                     LHAQAVDRLRTLMEGEPGMSPG"
BASE COUNT          233 a          605 c          539 g          240 t
ORIGIN      
        1 cccagctcgc cgctcggcgc ggcgccgtgg cagtccgggc agccgttgcc gccgccggcc
       61 gtcgaccggg tcgcgcggtc gtggcggatc atcgatctcc cggcgttcga tccggcgcag
      121 ttcgagcgcc agcgccggat cgtcctgcgc atcgcgctcg ccgtggtcgg cctcgtgatc
      181 gcgctcgcgc tgttcgccgg gcgctgctag gaaatcaccg cgggcgctag gtggcccgtg
      241 cctcgcgtgt caggatccgc gcgatgcccc ggtccaaggt cgctgtcctg cgagtccgcc
      301 ccgagcacgt cctcgaagac atcgatcgcc tgcacggcct ggccgaggtc gatcgcgcgc
      361 tgaccgccgg cgccaccacg atcctcaagg acaacatctc gtggcacttc ccgttccccg
      421 gcgccaacac cacgccatgg cagctcgagg gcacggtgaa gagcctgcgg gcgcgcggct
      481 tcaccgacct ggtgtgcgtc cagaacaaga ccgtggtgac cgacgcgttc aagggcgagg
      541 acctcaacgg ctacgtcccg atcttccggc accacggcgt gccggtccgg tacaacttca
      601 agcccgagga catgacgtgg tcggtgtacc ggccgcgcgc gaagatgcgc gtgctcgaca
      661 ccatcttccc cgagggcatc cagatccccg acgcgttcca cggcaccaac atcgtccacc
      721 tgccgaccgt caagtgccac atctacacca ccaccaccgg cgcgatgaag aacgcgttcg
      781 gcgggctgct caacacccgg cggcactaca cccacagctg gatccacgag accctggtcg
      841 acctgctcgc gatccagaag gagatccacg ccgggctgtt cgcggtgatg gacggcacca
      901 ccgcgggcga cggccccggg ccgcgcacga tgcggccggt gatcaaggac gtgatgctcg
      961 cgtcggccga tcaggtcgcg atcgacgcgg tcgcggcgtc gatgatgggg ttcgatccgc
     1021 tgaccatccc gtacctccgg ctcgccgacg aggccggcct gggcaacgcc cggcgcgagg
     1081 cgatcgacat cgccggcgac gccgcgctcg ccgaccagcg ctgggggttc tcggtcggcg
     1141 acaacggcgc gtcgctggtc ggcgacgtga tgtggttcgg cccgctcaag cggttccaga
     1201 acctgttctt ccggaccccg ctggtcaacc tgttcgtgct cggctccgag gcctaccacg
     1261 actactaccg ctggccgctg cgcgatcgcc gcgtgttcga gcactggcgg cagaccacgc
     1321 actggggccg gctgttcgat cgctaccagg ccgagggcac gctcggcgcg gccgcggtcg
     1381 cgacccaggc cggctgatcg ccgggtcagc tatctcatcc cgggctcatc ccgggctcgc
     1441 cctccatcag cgtgcgcagc cggtcgaccg cctgggcgtg cagccggctc gcccacgact
     1501 tcgacacgcc gagctcggcg ccggcctcga gcagactctt gccctcgaag tagtgcttgg
     1561 tgacgagcgc gcgcggccgg tcgggcagct gcgcgatcgc cacgcgcagc cgcgcgg
//
LOCUS       DASVOR010001760         2326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_426647,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001760 DASVOR010000000
VERSION     DASVOR010001760.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426647"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..1282)
                     /locus_tag="VF516_32775"
     CDS             complement(155..1282)
                     /locus_tag="VF516_32775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8112566.1"
                     /translation="MTRTEPAAAGPPAPEDPEAGDAGEPDDLDDDASGVDAFSLPPGA
                     DPTIDWGLRAHLHPDGSPRIIERHLMVPPELAGLRLDHFVKTQIPRLSRTRIQGIIAG
                     QLRRADGHAAKPATIVAAGDHFILRRAAQPEPPCPRTFGVVIRDPRLYVVDKPAGLPV
                     HASAKFYFNTLTRVLSERFPDEPELQICHRLDRETSGCLVVARDRAAAAAIKDAFATK
                     HRVRKQYLAVVHGQPPWDDEHTIDLALRVAQPGDPTRLTHVRMLAGPGGLPAITRVQV
                     ERRTTAHALVRCTLITGRQHQIRAHLAAAGYPIVGDKLYAHGDDAFLRYCNDGLVPEL
                     ARLFVLPRHALHAAAVSFPHPDGGSLSAEAPLPADLAGLLA"
     gene            complement(1269..>2326)
                     /locus_tag="VF516_32780"
     CDS             complement(1269..>2326)
                     /locus_tag="VF516_32780"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8112567.1"
                     /translation="YSIIEKIAPTATTVVIDGETGTGKEVVAQAIHSLSPRSRNDLVV
                     FDCGAVPPNLIESELFGHEKGSFTGAVMTRQGLFEQADGGTLFLDELGELPLDLQPKL
                     LRALEQREVRRVGSAKAAKVDVRIIAATNRNLEDEVRAGRFRQDLFYRLSVVRLHLPA
                     LRDRTDDIPLLIQHFLETGAFNRKPNAPAVRGVSREALTALQAYPWPGNVRELVNVVE
                     RAVSFCDHDLIQLSDLPDYIRTAKIPPPREIQPRRAATAPMSRDSAPLVSMNPNAPPP
                     TPPDELLAEGVTFKDAKERWVAAFERDYILQLLRRNNGNISHAARAADIDRKYFRKLM
                     KKYDIEAAGADDGDDAD"
BASE COUNT          297 a          767 c          925 g          337 t
ORIGIN      
        1 ccaccaccaa gcaagcaggg aatccggggg ctttcgcgat cgccgatcgt tgcggcgggg
       61 cgaaggtctg acatcgctat cacgttccgc gacagcttct tgccacctgg agccgccggc
      121 ggcgccgcgt gaggcagctc acggcggcca cccgtcaggc cagcaggccg gccaggtcgg
      181 ccggcagcgg tgcctcggcg ctgagcgagc cgccgtcggg gtgcgggaag ctcaccgcgg
      241 cggcgtggag cgcgtggcgc ggcagcacga acagccgcgc cagctcgggg accaggccgt
      301 cgttgcagta gcgcaggaac gcgtcgtcgc cgtgcgcgta gagcttgtcg ccgacgatcg
      361 ggtagccggc ggcggcgagg tgggcgcgga tctggtgctg gcggccggtg atcagcgtgc
      421 aacggaccag cgcgtgggcg gtggtccggc gctcgacctg gacccgcgtg atcgcgggca
      481 ggccgccggg gccggccagc atccgcacgt gggtcagccg ggtcgggtcg ccgggctggg
      541 cgacgcgcag cgcgagatcg atcgtgtgct cgtcgtccca cggcggctgg ccgtgcacca
      601 ccgcgaggta ctgcttgcgc acgcggtgct tggtggcgaa cgcgtccttg atcgcggcgg
      661 cggcggcgcg atcgcgcgcg accaccaggc agcccgaggt ctcgcggtcg agccggtggc
      721 agatctggag ctcgggctcg tcggggaagc gctcggacag cacgcgggtc agggtgttga
      781 agtagaactt ggccgacgcg tggaccggca ggccggccgg cttgtcgacg acgtagagcc
      841 gcggatcgcg aatgacgacg ccgaacgtgc gcgggcacgg cggctcgggc tgggcggcgc
      901 gccgcaggat gaagtgatcg ccggcggcga cgatcgtcgc gggcttggcc gcgtggccgt
      961 cggcgcggcg cagctggccg gcgatgatgc cctggatccg cgtgcgcgac agccgcggga
     1021 tctgggtctt gacgaagtga tcgagccgca gcccggcgag ctcgggcggg accatcaggt
     1081 ggcgctcgat gatccgcggc gagccgtcgg ggtggaggtg cgcccgcagg ccccagtcga
     1141 tcgtggggtc ggcgccgggc ggcaggctga acgcgtcgac gccggacgcg tcgtcgtcga
     1201 ggtcgtccgg ctcgccggca tcgccggcct ccggatcctc gggcgccggc gggcccgcgg
     1261 ccgccggctc agtccgcgtc atcgccgtcg tcggcgccgg cggcctcgat gtcgtacttc
     1321 ttcatcagct tgcggaagta cttgcgatcg atgtccgcgg cgcgcgcggc gtgcgagatg
     1381 ttgccgttgt tgcgccggag cagctggagg atgtagtcgc gctcgaacgc cgcgacccag
     1441 cgctccttgg cgtccttgaa cgtgacaccc tcggcgagca gctcgtcggg cggcgtcggc
     1501 gggggcgcgt tggggttcat cgacaccagc ggcgcgctgt cccggctcat cggcgcggtg
     1561 gcggcgcggc ggggctggat ctcgcgcggc ggcgggatct tggcggtccg gatgtagtcg
     1621 ggcagatccg agagctggat caggtcgtga tcgcagaacg acaccgcgcg ctcgacgacg
     1681 ttgaccagct cgcggacgtt gcccggccac gggtaggcct gcagcgcggt cagcgcctcg
     1741 cgcgacacgc cgcgcaccgc cggggcgttg ggcttgcggt tgaacgcgcc ggtctccagg
     1801 aagtgctgga tcaacagcgg gatgtcgtcg gtgcggtcgc gcagcgccgg caggtgcagc
     1861 cggaccacgc tgagccggta gaacaggtcc tggcggaacc ggccggcgcg gacctcgtcc
     1921 tcgaggttgc ggttggtcgc ggcgatgatc cggacgtcga ccttggcggc cttggccgag
     1981 ccgacccggc ggacctcgcg ctgctccagc gcgcgcagca gcttgggctg gaggtcgagc
     2041 ggcagctcgc cgagctcgtc gaggaatagc gtgccgccgt cggcctgctc gaacaggccc
     2101 tggcgggtca tcaccgcgcc ggtgaagctg cccttctcgt ggccgaacag ctcgctctcg
     2161 atcaggttgg gcggcaccgc gccgcagtcg aacacgacca ggtcgttgcg cgagcgcggc
     2221 gacagcgagt ggatcgcctg ggcgacgacc tccttgccgg tgccggtctc gccgtcgatc
     2281 accacggtgg tcgcggtcgg cgcgatcttc tcgatgatcg agtaga
//
LOCUS       DASVOR010001761         4599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_397394,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001761 DASVOR010000000
VERSION     DASVOR010001761.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4599
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397394"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VF516_32785"
     CDS             <1..1082
                     /locus_tag="VF516_32785"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEX8112568.1"
                     /translation="RREAGVTDPPRRVTAPLEVPSTEEMLQRACDVLRRAERILLTSH
                     RRPDGDGTGSMAGLASLLRARGKTAVIYSVDPIARRYKWLPLVGSTVQTISFDERFDC
                     TVVVDCADATLLGDTLPPPEVRGRLITLDHHASGHPFGDIAVWDPAAAAVGVLVHRIA
                     VREGWPVTIEAAIPLYVSLISDTGGFRHANTNAEALSVAAELIRAGVVPSAIAASLEE
                     RPSPGKLRLLGSVLATLELHCAGRVGMLTVTTDMVEGARASWEDIEGMVNWARNVEGV
                     QVGVLLTTAKGGGVRVSMRSRSEKVDVGKVCLALGGGGHPGAGGCHIAADLAVTKDRV
                     VRALDQAFNRNSRPVLEPAPEPAGE"
     gene            1103..2059
                     /gene="truB"
                     /locus_tag="VF516_32790"
     CDS             1103..2059
                     /gene="truB"
                     /locus_tag="VF516_32790"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEX8112569.1"
                     /translation="MPGDIPGDIPGNIPGNIHGILVLDKPAGMTSAAAVDRVRRTLGI
                     ARAGHGGTLDPIATGVLPICLGAGTKLAQFLLAEDKAYLAEGVLGCETDTLDRTGRIV
                     AERPGVAVTREALAAALAAHLGEQTQIPPMYSAIKQGGVRLYDRARAGEEVERAPRPI
                     RIDRLELLAFEPPRFRLAIACSKGTYVRSLVADLGTAVGAGAHLAELRRIRSGRFTIE
                     QAVPLDRLGDAELVPPEHALELERVTVPRALVAPVLSGARLPAVTWDVEAQRFQLLDE
                     DGRLLAVAHTEAGRTVYDRVFPELVRASKDAAGRRSSGTPGA"
     gene            2144..2413
                     /gene="rpsO"
                     /locus_tag="VF516_32795"
     CDS             2144..2413
                     /gene="rpsO"
                     /locus_tag="VF516_32795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEX8112570.1"
                     /translation="MPVSTVRKAETISKFQQHQGDTGSPEVQVALLSERITHLTEHLK
                     NNHKDHHSRRGLLKMVGQRRALLDYLKRKNLERYSKLINTLGLRR"
     gene            3138..>4599
                     /gene="pnp"
                     /locus_tag="VF516_32800"
     CDS             3138..>4599
                     /gene="pnp"
                     /locus_tag="VF516_32800"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEX8112571.1"
                     /translation="MPFVRETAQVGGKEIIVETGKWAKQAGGSVVVRCGDSMVLVTAS
                     GSPQPRDIDFMPLTCEYRENFYSSGKIPGSFFRREGRSTNEEILICRLMDRPVRPLFP
                     KGWFIDTQIIANVVSFDKENPTDVLAMTGASTALHVSDLVWGGPIAGVRVGRIDGEFV
                     ANPTFAEREKSDMDIVVAASKDAIMMVEGEMHEMQENVVIDALLFAHQAVQPLIELQE
                     RLRLANGKDKRPFTPPVADEALAAQVKELAWNRLGEAMTIKDKHKRRHAVSTLHLEVR
                     GALTAEGQPWAGRSKEVDGAFAKLETKWARGHTLATRSRIDGRAPDEIRAISIETGVL
                     PRAHGSALFTRGETQALVAVTLGTKYDEKKLDTLLGDRKKTFYMDYNFPPFSTGEVKP
                     LRGQSRREVGHGFLAERSVEAVVPIYEDFPYTIRVVSDILESNGSSSMASVCGGSLAL
                     MDAGVPTKAPIAGIAMGLMKEGNDYVVLSDILGDEDH"
BASE COUNT          711 a         1633 c         1606 g          649 t
ORIGIN      
        1 agcgacgaga ggccggcgtg accgatccgc cccgccgcgt gaccgcaccg ctcgaggtgc
       61 cgtccaccga ggagatgctg cagcgcgcgt gcgacgtgct gcgccgcgcc gagcgcatcc
      121 tgctcaccag ccaccgccgc cccgacggcg acggcaccgg ctcgatggcg gggctggcct
      181 cgctgctgcg cgcgcgcggc aagaccgcgg tgatctactc ggtcgacccg atcgcgcggc
      241 gctacaagtg gctgccgctg gtcggctcga cggtccagac catctcgttc gacgagcggt
      301 tcgactgcac ggtcgtggtc gactgcgccg acgccacgct gctcggcgac acgctgccgc
      361 cgcccgaggt ccgcggccgg ctgatcacgc tcgatcacca cgccagcggc cacccgttcg
      421 gcgacatcgc ggtgtgggac cccgcggcgg cggcggtcgg cgtgctggtc caccggatcg
      481 ccgtgcgcga gggctggccg gtcacgatcg aggccgcgat cccgctgtac gtgtcgctga
      541 tcagcgacac cggcgggttc cgccacgcca acaccaacgc cgaggccctg tcggtcgccg
      601 ccgagctgat ccgcgccggg gtcgtgccgt cggcgatcgc ggcgtcgctc gaggagcggc
      661 cgagcccggg caagctgcgc ctgctcggct cggtgctggc gacgctggag ctgcactgcg
      721 ccggccgggt cggcatgctg accgtcacca ccgacatggt cgagggcgcc cgcgcgtcgt
      781 gggaggacat cgagggcatg gtcaactggg cgcgcaacgt cgagggcgtc caggtcggcg
      841 tgctgctcac caccgccaag ggcggcggcg tccgggtctc gatgcggtcg cgcagcgaga
      901 aggtcgacgt cggcaaggtc tgcctggcgc tcggcggcgg cggtcacccc ggcgccgggg
      961 gctgccacat cgcggccgac ctcgcggtca ccaaggaccg cgtggtgcgc gcgctcgacc
     1021 aggcgttcaa tcgcaacagc cgccccgtgc tggagccggc gccggagccc gcgggcgaat
     1081 gaccgcggcc gcgcgctgag ccatgcccgg cgacattcct ggcgacattc ccggcaacat
     1141 tcccggcaac attcacggca tccttgtcct cgacaagccg gccgggatga cctcggccgc
     1201 cgcggtcgac cgcgtgcgcc gcacgctcgg gatcgcgcgg gccggccacg gggggacgct
     1261 cgacccgatc gcgaccggcg tcctgccgat ctgcctgggc gccggcacca agctcgcgca
     1321 gttcctgctc gccgaggaca aggcgtacct cgccgagggc gtgctcgggt gcgagaccga
     1381 cacgctcgac cgcaccggcc ggatcgtcgc cgagcgcccg ggggtcgcgg tgacgcgcga
     1441 ggcgctggcc gcggcgctcg ctgcccacct cggcgagcag acccagatcc cgccgatgta
     1501 ctcggcgatc aagcagggcg gggtccggct ctacgatcgg gcgcgggccg gcgaggaggt
     1561 cgagcgcgcg ccgcgaccga tccggatcga tcggctcgag ctgctggcgt tcgagccgcc
     1621 caggttccgg ctcgcgatcg cgtgcagcaa gggcacctac gtccgcagcc tggtcgccga
     1681 tctcggcacc gcggtggggg ccggcgcgca cctggccgag ctccgccgga tccgcagcgg
     1741 ccggttcacc atcgagcagg cggtgccgct ggaccgcctg ggggacgcgg agctcgtgcc
     1801 gcccgagcac gccctggagc tcgagcgggt caccgtgccg cgcgcgctgg tcgcgccggt
     1861 gctgagcggc gctcggctgc ccgctgtcac gtgggatgtc gaagcccagc gattccagtt
     1921 gcttgacgag gatggcaggc tgctggcggt ggcccacacc gaggccgggc gcacggtcta
     1981 cgaccgcgtg ttccccgagc tggtccgcgc gagcaaggac gccgccggcc ggcgcagttc
     2041 gggcaccccc ggcgcttgac gctcgccccc ctttccccca atctgcgctc gaccgcggcg
     2101 cacgttccat ccggggcgcg cccgccggcg actggagtac cccatgcctg tttccaccgt
     2161 acgcaaggcc gagacgatct cgaagttcca gcagcaccag ggcgataccg gatcgcccga
     2221 ggtccaggtc gcgctcctgt ccgagcgcat cacgcacctg accgagcacc tcaagaacaa
     2281 ccacaaggac caccacagcc ggcgcggcct gctgaagatg gtcggccagc gccgcgcgct
     2341 gctggattac ctcaagcgca agaacctcga gcgctacagc aagctgatca acacgctcgg
     2401 cctgcgccgc tagcgcgagc gcgggtcgac ccgcgctccg gcggcggggc ggtcagaggt
     2461 ctgccacagc gccctcggga tccagcgacg atctcgggaa tccaagattt tcgcgtccgg
     2521 caggcgtcgg gccagatccc gcgccgtggt ggtcttgccc atgccccatg caagcggtgg
     2581 ttgacgtcga cggtttgacc ttgacgtcga ccccaccctc cacctcgacg tcgacttccg
     2641 gatctgcgtc cacatcgacc acaccctagc cgcgaggctc atcggctccg acggccgacg
     2701 gttgcttgac tccggagtca aggtgcaagg ttcagggtgg ggtcagacgt catcgtcgcc
     2761 gtcaacctca acgttcgggt caaggtcatt gtcgaggaca aggtcaaaga attccggaat
     2821 cgcgtccggc ctccgggatc gacaagtctt cccgccagcg gtacaggatc cgccagatca
     2881 gccaacccta ctggagcgat ccgtgtcacg gaactcgtga gggttacggt cttttctttc
     2941 ggtccggagt gtgccatacc gcccggcgaa ccaagaacct gccactgtag cgtcgtcgaa
     3001 cgttggaatc gcgctggttt gatcgttccg tcgagtcgct cgacaagagc tgtgcccccc
     3061 ggggccgtcg agaggttgga cagagggacc aggccacgaa cccccggccg gcgacgtgac
     3121 caccatcgag gacacgtatg ccgttcgttc gtgagaccgc ccaagtcggc ggcaaggaga
     3181 tcatcgtcga gaccgggaag tgggccaagc aggccggcgg ctcggtcgtc gtccgctgcg
     3241 gcgacagcat ggtgctggtc actgcgtcgg gatcgccaca gccgcgcgac atcgacttca
     3301 tgccgctgac ctgcgagtac cgcgagaact tctactcgtc gggcaagatc ccgggcagct
     3361 tcttccgccg cgaaggccgg tcgaccaacg aggagatctt gatctgccgc ctgatggacc
     3421 gcccggtccg cccgctgttc cccaagggct ggttcatcga cacccagatc atcgcgaacg
     3481 tggtgtcgtt cgacaaggag aacccgaccg acgtgctggc gatgaccggc gcgtcgacgg
     3541 cgctgcacgt ctcggatctg gtctggggcg gcccgatcgc cggcgtgcgg gtcggccgga
     3601 tcgacggcga gttcgtcgcc aaccccacgt tcgccgaacg cgagaagtcg gacatggaca
     3661 tcgtggtcgc ggccagcaag gacgcgatca tgatggtcga gggcgagatg cacgagatgc
     3721 aggagaacgt cgtgatcgac gccctgctgt tcgcgcacca ggcggtccag ccgctgatcg
     3781 agctccagga gcgtttacgc ctggcgaacg gtaaggacaa gcggccgttc accccgccgg
     3841 tggccgacga ggcgctcgcc gcgcaggtca aggagctggc gtggaaccgg ctcggcgagg
     3901 cgatgaccat caaggacaag cacaagcggc gccacgcggt gtcgacgctg cacctcgagg
     3961 tccgcggcgc gctgaccgcc gagggccagc cgtgggccgg caggtccaag gaggtcgacg
     4021 gcgcgttcgc caagctcgag acgaagtggg cgcgcggcca cacgctcgcg acccgcagcc
     4081 ggatcgacgg ccgcgcgccc gacgagatcc gcgcgatctc gatcgagacc ggcgtgctgc
     4141 cgcgcgcgca cggctcggcg ctgttcaccc gcggcgagac ccaggcgctg gtcgccgtca
     4201 cgctgggcac caagtacgac gagaagaagc tcgacacgct gctcggcgac aggaagaaga
     4261 cgttctacat ggactacaac ttcccgccgt tctcgaccgg cgaggtcaag ccgctgcgcg
     4321 gccagtcgcg ccgcgaggtc ggccacggct tcctcgccga gcgcagcgtc gaggcggtcg
     4381 tgccgatcta cgaggacttc ccgtacacga tccgcgtggt gtccgacatc ctcgagtcca
     4441 acggcagctc gtcgatggcg tcggtgtgcg gcggctcgct cgcgctgatg gacgccggcg
     4501 tgccgaccaa ggcgccgatc gccggcatcg cgatgggcct gatgaaggag ggcaacgact
     4561 acgtcgtgct gtccgacatc ctcggcgacg aggaccacc
//
LOCUS       DASVOR010001762         2277 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_280280,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001762 DASVOR010000000
VERSION     DASVOR010001762.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280280"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            712..>2277
                     /locus_tag="VF516_32805"
     CDS             712..>2277
                     /locus_tag="VF516_32805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HEX8112572.1"
                     /translation="MSHLDSSAAALHDKYRPDIDGLRAVAVGLVVVFHAFPGVLPHGF
                     IGVDIFFVISGFLISTMIFRQVRAGTFSIGDFYARRIRRLFPALIVVLAAVYMAGWRL
                     LLPGELAQLGKHVAGGAGFGANLLLWAETGYFDATAESKPLLHLWSLGIEEQFYLIWP
                     VLLRLLWRPRFNLLTGALVIASASFYFNVIHVRIHADAVFYLPATRLWELLSGAVVGY
                     LNLRATGRSSRFLARLGRDLKHVLYDRESTISPQQVLCSFGSIVGVSCIVIALVAAMK
                     YFPGQFALWPVLGAALIIAAGPDALPNRLLRMRGFVWVGRISYPLYLWHWPMLCFARI
                     GWPAGGASVAARLALVAGSVVLAWLTYRFIEKPVRYSDRHQTAKMESLIASMTVLLVL
                     GVATWWLDGFPARFPPAVRELTSIKSEFPSSYRYRQCFLSQEQEPSTFAGCPVILRSA
                     PRQQTILLWGDSYAAHFMMGLQSTVPATAAIVQRTAAGCPPIPGLDLPDFPLCKAVNA
                     SVIASLPEIKPDLV"
BASE COUNT          354 a          720 c          740 g          463 t
ORIGIN      
        1 atctgcgatc cgcgctgccg cgctacatgc tgccgcgtcg cgtgcacctg ctcgaggtga
       61 tcccgcgcaa ccccaacggc aagatccgga tcgacgcgct ccgcgcgctg tcttctgcac
      121 ctgcgtgagc gcacgccgcg cccgcggtgc gctcgacgga cacccggacg gcgcctgcgc
      181 tgacgtccta cacggcgatg gcagagcaca tggtgcgcag ctcgctcctc ggggtcccga
      241 cagtaccggc ggaagaactc ggtgagtccg ttggaccgct cgagatgccc gcgccatatt
      301 ccgctgtgcc gtgagcgccg caggctgcac tcggtcatcc ccgacgagcc gaccagccac
      361 gtagacctga cgtcgatcga gcacctggag gacgcaccgg tcgctcaccg cggcggattg
      421 ccgtcggccg gccgatactc gcaccatggc ggacgctgga atggctgccg gtaaagaggc
      481 ttaccaagat actgctgttc aaaaatctgt caccggtgga tgaaattgcc agagattacg
      541 tcacagctgc acgccgctcg agggcatatt agaacaggga ggccgatgta gaatgccgcg
      601 gtcaagtcct cagatgatcg ataaacaggc agtatccctc gctccagata tcgagcgcag
      661 aatccgtgaa catgtggcac ggcgagtggg ttttgaccag ctggagtcgt cgtgagtcat
      721 ctcgatagca gcgcggcggc gctgcacgac aagtaccggc cggacatcga tggcctgcgt
      781 gcggtcgcgg tcggcctcgt cgtggtgttt catgcgttcc cgggagttct gccccacggc
      841 ttcatcgggg tcgatatctt cttcgtgatc tcggggtttc tgatctccac gatgatcttc
      901 cggcaggttc gggcggggac gttctcgatc ggcgacttct acgcccgccg gatccgccgg
      961 ctgttccccg cgctgatcgt cgtgctcgct gcggtctaca tggcagggtg gcggctgttg
     1021 ctccccggcg agcttgcgca gctcggcaaa catgtcgccg gtggtgccgg gttcggcgcg
     1081 aatctgctgc tatgggccga aaccggctac ttcgatgcga cggcggagag caagccgctg
     1141 ctgcacctgt ggtctctcgg catcgaagaa cagttttatt tgatctggcc ggtgttgttg
     1201 cgattgctgt ggcgtccgcg attcaatctc ctcacgggcg cgctcgtgat cgcgagtgcc
     1261 tccttctact tcaacgtcat tcatgttcga atccacgctg acgccgtgtt ctatctcccg
     1321 gcgacccggc tgtgggagct gctgagcggc gcggtggtcg gctacctgaa cctgcgcgcc
     1381 accgggcgat cgagccggtt cctggcgcgc ctcggacgtg acctcaagca cgtgctctac
     1441 gatcgagaat cgacgatctc gccccagcag gtgttgtgca gcttcggatc gatcgtgggg
     1501 gtgtcgtgta tcgtcatcgc cctcgtcgct gcgatgaaat actttccagg acagttcgcg
     1561 ttgtggccag tgctgggggc cgcgctgatc atcgccgcgg ggccggacgc gcttcccaac
     1621 aggctgctga gaatgcgtgg gtttgtctgg gtggggcgga tcagctatcc gttgtacctg
     1681 tggcactggc cgatgctgtg cttcgcgcgg atcggatggc cagcgggcgg ggcgtcggtc
     1741 gcggcgaggc tcgctctggt cgcaggcagc gtggtcctgg cgtggctcac ctatcgcttc
     1801 atcgagaagc cagtgcgata ctcggatcgt catcaaacgg ccaagatgga gagtctgatc
     1861 gcgtcgatga cagtccttct cgtgctgggg gtcgcgacgt ggtggctcga tggatttcct
     1921 gcgcggttcc cgccggcggt ccgtgagctc accagtatca agtccgagtt cccgagctcg
     1981 taccgctacc ggcagtgttt tctcagccag gaacaggagc cgtcgacatt cgccggatgc
     2041 ccggtcattc tgcgctcggc gccacgccag cagacgatct tgctctgggg ggactcctat
     2101 gccgcccact tcatgatggg cttgcagagc accgtgcctg cgacggcggc gatcgtgcag
     2161 cgcaccgcgg caggctgccc accgatcccc ggactcgact tgcccgactt tccgctgtgc
     2221 aaggcggtca acgcgtcggt catcgcgtcg ctccccgaga tcaagccgga cctcgtg
//
LOCUS       DASVOR010001763         2861 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_368946,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001763 DASVOR010000000
VERSION     DASVOR010001763.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368946"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="VF516_32810"
     CDS             complement(<1..733)
                     /locus_tag="VF516_32810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEX8112573.1"
                     /translation="MQAHDLSEHVHHEDEQAHNRVGERRTRWVIAITAVMMLGELVVG
                     WWTGSVALQADGWHMGSHVLALGLTLAAYWYARTRAGRDTFSFGTGKVYALAGYTSGV
                     LLVLVAAWMAYEGGHNLITHPDVDYRDSLPVAALGFVVNLVSTALLSRGHHYGHGHAG
                     HAHAGHDHAGHDHAGHDHVHGGGQATARPAPRPGTLDFNLRAAYIHIFADAMTSLLAV
                     AALALGWCAGLWYLDPLMGLIGGGVI"
     gene            784..1242
                     /locus_tag="VF516_32815"
     CDS             784..1242
                     /locus_tag="VF516_32815"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEX8112574.1"
                     /translation="MAIRALRAAGVAFVPHLYPYEPRGGTQASSEALGVLEHSVVKTL
                     IFETERGEPLCILMHGDREVSVKQLARLVGAKSVAPCAPEVADRHSGYQVGGTSPFGL
                     RRAMPIYMQSTILELPRIYINGGARGFLVDIDPRDAQRVLAPVLVDVAIG"
     gene            1318..1857
                     /locus_tag="VF516_32820"
     CDS             1318..1857
                     /locus_tag="VF516_32820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112575.1"
                     /translation="MRGNLALRAVPPPIQSGDPDDETGEEMAPSSGSGGPGAGALRAG
                     DTGAEAPAIRRGRLLIIDDEVVFSGSLRRLFSNEHDVTVVNRGTEALGRLRAGERFDA
                     ILCDLLMPEVTGVELYTELRQIAPDQANCMIFLTGGAFSESSQRFLDGVSNRWFEKPC
                     NLDLLRAAVREVVVRNRSR"
     gene            1955..>2861
                     /locus_tag="VF516_32825"
     CDS             1955..>2861
                     /locus_tag="VF516_32825"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEX8112576.1"
                     /translation="MRPLQVLVVDDDRMQLRALERILRDQHEVDLTLADNALDAMIAV
                     GALKPDLVIMDILMPGLDGIEACRRLKANPETRDIQIVLASAMMTPELDRAARHAGAR
                     RVVAKPVDLAELVGAAAAQNARAASSQPEPVPTIRGANLIVEMLAEAGVEIVFGLPGG
                     AISPVHDALLDSKLRVVTTRHESGAMFAAAGYARATGKLAVVAVTSGPGVLNALTGLA
                     TAWCDGLPVLLLVGEVPRPAHGKGVLQDGSAHGLQIVEMARYITKVAAEVPRPSALPH
                     LLRRAISTTQSGRRGPALLTLPMDVT"
BASE COUNT          427 a          977 c         1014 g          443 t
ORIGIN      
        1 cgatcacgcc gccgccgatc aggcccatca gcgggtcgag gtaccacagg cccgcacacc
       61 agccgagcgc cagcgcggcg accgccagca gcgaggtcat cgcgtccgcg aagatgtgga
      121 tgtaggcggc gcgcaggttg aagtcgagcg tgcccggccg gggcgcgggg cgcgcggtgg
      181 cctgaccacc gccgtggaca tggtcgtgac cggcatggtc gtgaccggca tggtcgtgac
      241 cggcgtgcgc gtgaccggcg tggccgtggc cgtagtggtg gccacggctg agcagcgcgg
      301 tcgacacgag gttgaccacg aagcccagcg cggcgaccgg cagcgagtcg cggtagtcga
      361 cgtccggatg ggtgatcagg ttgtggccgc cctcgtaggc catccacgcc gcgaccagca
      421 cgagcagcac gccgctggtg tagccggcga gcgcgtagac cttgccggtg ccgaagctga
      481 acgtgtcgcg cccggcccgg gtccgcgcgt accagtaggc ggccagcgtc aggccgagcg
      541 cgaggacgtg gctgcccatg tgccagccgt cggcctggag cgcgaccgag ccggtccacc
      601 agccgacgac gagctcgccg agcatcatca cggccgtgat cgcgatgacc caccgcgtgc
      661 gccgctcgcc gacccggttg tgcgcctgct cgtcctcgtg gtggacgtgc tcggacaggt
      721 catgcgcttg catggcgccg gcagtctagc ccatcatctc gtgtgcccaa gcagccggtc
      781 acgatggcga tccgcgcgct gcgggccgcg ggggtcgcgt tcgtcccgca cctctacccg
      841 tacgagccgc gcggcgggac ccaggcatcg agcgaggcgc tcggcgtcct ggaacacagc
      901 gttgtcaaaa cgttgatctt cgagaccgag cgcggcgaac ccttgtgcat cctcatgcat
      961 ggcgatcgtg aagtctcggt gaagcagctc gcgcgcctgg tcggggccaa gagcgtcgcg
     1021 ccgtgtgccc cggaggtcgc cgaccgtcat tcgggatatc aggtgggggg tacctcacct
     1081 ttcggcctgc gacgcgcaat gccgatctac atgcagtcga cgatcctcga gctaccgcgg
     1141 atctacatca acggcggcgc gcgcggcttc ctggtcgata tcgacccgcg cgatgcccag
     1201 cgcgtgctgg cgcccgtcct ggtcgacgtc gccatcggct gaatggtcgg acacgttccc
     1261 ttgcatccgg acagccattg aatcaccctc cttgacattg tgaccagtgc tggccgtgtg
     1321 cgtggcaacc tcgctctccg tgccgttccg ccgcccatcc agtcgggcga ccccgacgac
     1381 gagaccggcg aggagatggc cccgtcatcg gggtcgggag ggccgggcgc gggtgcgctg
     1441 cgcgccggcg atacgggcgc cgaggccccg gccatccggc gcggccggct gctgatcatc
     1501 gacgacgaag tcgtgttctc cgggtcgctc cgtcggctgt tctcgaacga gcacgacgtc
     1561 accgtggtca accggggaac cgaggcgctc ggccggttac gggcgggtga gcggttcgac
     1621 gcgatcttgt gcgatctcct gatgccggaa gtcacgggcg tcgagctgta cactgagttg
     1681 cgccagatcg cccctgacca ggcgaattgc atgatcttcc tcaccggggg cgcgttctcg
     1741 gagagctcac agcgattcct cgatggagtc tcgaatcgct ggttcgagaa gccgtgcaat
     1801 ctggacctgc tgcgcgccgc agtgcgcgag gtcgttgtac gcaaccgcag tagataggcg
     1861 cacccgagat ttggttggtt ccgtgggggg caatcggcgg tataagccga tagtgtccct
     1921 tgcaccgtcg acagggccgc ggaccgccgc tggcatgcgc ccgctgcagg tgcttgtagt
     1981 cgacgacgat cgcatgcagc tccgcgcgct cgagcgcatc ctgcgcgatc agcacgaggt
     2041 cgatctcacg ctggcggaca atgcgctcga cgcgatgatc gcggtcggag cgctcaagcc
     2101 cgatctcgtg atcatggaca ttctcatgcc cgggctcgac gggatcgagg cctgccggcg
     2161 gctcaaggcg aaccccgaaa cccgcgatat ccagatcgtc ctggcgagcg ccatgatgac
     2221 acccgagctg gaccgcgcgg cgcgtcacgc cggcgcacgg cgcgtggtgg ccaagccggt
     2281 cgacctcgcc gagctcgtcg gggcggcggc ggcgcaaaat gcgcgcgccg cgagcagcca
     2341 gcccgagccg gtcccgacga tccggggcgc caacctcatc gtcgagatgc tcgccgaggc
     2401 gggagtcgag atcgtgttcg gcctgccggg cggggcgatc tccccggtcc atgacgcgct
     2461 gctcgatagc aagctgcggg tcgtcaccac ccgccacgag agcggggcga tgttcgcggc
     2521 tgccggatat gcacgtgcga ccggcaagtt ggccgtggtc gccgtgacct cggggcccgg
     2581 ggtgctcaac gcgctgaccg ggctggccac ggcgtggtgc gatggactcc cggtcctgct
     2641 gctggtcggc gaggtgccgc ggccggccca cggcaagggc gtgctacagg acgggtccgc
     2701 ccatggcctg caaatcgtcg aaatggctcg ttacatcacc aaggtcgcgg ccgaggtgcc
     2761 gcggccgagc gcgctgcccc acctgctccg ccgcgcgatc tcgaccaccc agtcgggccg
     2821 gcgcggtccg gccctgctca cgctcccgat ggacgtgacc c
//
LOCUS       DASVOR010001764         3331 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_19389,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001764 DASVOR010000000
VERSION     DASVOR010001764.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19389"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VF516_32830"
     CDS             complement(<1..203)
                     /locus_tag="VF516_32830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112577.1"
                     /translation="MPPAGTRLPRPAGADAVPRLITPGPPPEWRFGELTVVIGAARAE
                     LRYAREPVGSSPPAPDAIAAAVER"
     gene            complement(468..1268)
                     /locus_tag="VF516_32835"
     CDS             complement(468..1268)
                     /locus_tag="VF516_32835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX8112578.1"
                     /translation="MTTADPHTLRLTLHIWRQPDRASRGKMVTYQVSGVSTHASFLEM
                     LDILNEQLIAKGEEPVAFDHDCREGICGMCSLMINGRPHGGKPATTTCQLHMRLFRDG
                     DEIWIEPWRAHAFPVIKDLCVDRSAFDRVIAAGGFITANCGSAPDANDILVAKDRADR
                     AMDAAACIGCGACVAACPNGSAMLFVAAKLAHLHQLPQGQPERAHRTQDMVSAMDAAG
                     FGACTNHYECEAACPKAISRDVIAQMNRDYARAALVARDREVKSDGTG"
     gene            complement(1276..3192)
                     /locus_tag="VF516_32840"
     CDS             complement(1276..3192)
                     /locus_tag="VF516_32840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX8112579.1"
                     /translation="MELNARIPGGPIEQKWDHHRFAMTLVNPANKRKYKVIVVGSGLA
                     GGAAAATLAELGYDVACFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSIHRLFYDTVK
                     GGDFRARESNVHRLAEVSVDIIDQCAAMGVPFAREYGGTLANRSFGRAQVSRTFYARG
                     QTGQQLLLGVYAALSRQIGVSKVTMFPRTELLDIVVDNGRAVGVVVRDLITGAISSHS
                     AHAVVLATGGYGNAFYLSTNAKGCNVTATYRAYKRGAGFANPCFTQIHPTCIPVAGDY
                     QSKLTLMSESLRNDGRVWVPKSAGDVARHPDQIPEEARDYYLERKYPSFGNLSPRDIA
                     SRAAKEQCDEGRGVGPGGRGVYLDFRDAIRRDGEDRVREKYGNLFEMYERITGEDPYK
                     VPMRIYPAVHYTMGGLWVDYHLQTTIPGLFCLGEANFSDHGANRLGASALMQGLADGY
                     FIIPYTLGHYLAGVPAQSLADPASAPFAAAEAEIAARLDRLVNVRGTRSVDAFHKELG
                     HILWEDCGMARSRQSLTDALAKIPALREQFWREVTVLGERDSFNQSLEKAGRVADFLE
                     FAELMCSDAVVREESCGGHFRVEHQADGEAVRDDVSFCHVAVWEHAGDGAAPVRHIEP
                     LAFENVKLATRSYK"
BASE COUNT          462 a         1097 c         1252 g          520 t
ORIGIN      
        1 cgttcgaccg cggcggcgat cgcatcgggc gcgggcggcg acgatccgac cggctcgcgc
       61 gcgtagcgca gctcggcccg cgcggcgccg atgaccacgg tcagctcccc gaaccgccac
      121 tcgggcggcg gacccggcgt gatcaaccgc ggaacggcgt cagccccggc ggggcgcggg
      181 agcctggtgc ccgccggcgg cattgcaaga gcgtacaccg tgcgcggcgt acccgatacc
      241 tcgggtttgt ccggcctcga aggcgcgcag ccgtgtgagc cgccacctgc tgcggcagca
      301 cactggcgta ctggcgcata ggcgtcgcgc tggaggctca tggctccttt gtggccccag
      361 ggaatttgtc caacaggctg ttggacgaat cctgggcttg tgcttcgtcg ccttcgtcac
      421 aggatcacct ccagctcggc aaggtcgttc gcagctccgg cgctggatca gcccgtgccg
      481 tcggacttga cctcgcggtc gcgcgcgacc agggcggcgc gggcgtagtc gcggttcatc
      541 tgcgcgatga cgtcgcgcga gatcgccttg gggcacgcgg cctcgcactc gtagtggttg
      601 gtgcacgcgc cgaacccggc ggcgtccatc gccgagacca tgtcctgggt gcggtgggcg
      661 cgctcgggct ggccctgcgg cagctggtgg aggtgggcga gcttggcggc gacgaacagc
      721 atcgccgagc cgttggggca ggccgcgacg cacgcgccgc agccgatgca ggcggcggcg
      781 tccatcgcgc ggtcggcgcg gtccttggcg accaggatgt cgttggcgtc gggcgccgag
      841 ccgcagttgg cggtgatgaa gccgccggcg gcgatcacgc ggtcgaacgc cgagcggtcg
      901 acgcacaggt ccttgatcac cgggaacgcg tgggcgcgcc acggctcgat ccagatctcg
      961 tcaccgtcgc ggaacaaccg catgtggagc tggcaggtcg tggtcgccgg cttgccgccg
     1021 tgcgggcggc cgttgatcat cagcgagcac atgccgcaga tgccctcgcg gcagtcgtgg
     1081 tcgaacgcga ccggctcctc gcccttggcg atcagctgct cgttgaggat gtcgagcatc
     1141 tcgaggaacg aggcgtgggt cgatacgccg gacacctggt aggtcaccat cttgccgcgg
     1201 ctcgcgcggt cgggctggcg ccagatgtgg agcgtgaggc gcagggtgtg ggggtcggcg
     1261 gtggtcactg gtagatcact tgtagcttcg ggtggcgagc ttgacgttct cgaacgccag
     1321 cggctcgatg tggcggaccg gcgcggcgcc gtcgccggcg tgctcccaga ccgcgacgtg
     1381 gcagaagctg acgtcgtcgc gcacggcctc tccgtcggcc tggtgctcga cgcggaagtg
     1441 cccgccgcag ctctcctcgc ggaccaccgc gtcgctgcac atcagctcgg cgaactcgag
     1501 gaagtcggcg acccggccgg ccttctcgag cgactggttg aagctgtcgc gctcgccgag
     1561 cacggtgacc tcgcgccaga actgctcgcg cagcgccggg atcttggcca gcgcgtcggt
     1621 cagcgactgc cggctgcgcg ccatgccgca gtcctcccac aggatgtggc cgagctcctt
     1681 gtggaacgcg tcgaccgagc gagtgccgcg gacgttgacc aggcggtcga gccgggccgc
     1741 gatctcggcc tcggcggccg cgaacggcgc gctcgcgggg tcggcgaggc tttgcgccgg
     1801 cacgccggcg aggtagtggc cgagggtgta cgggatgatg aagtagccgt cggcgaggcc
     1861 ctgcatcagc gcggacgcgc cgagccggtt ggcgccgtgg tcggagaagt tggcctcgcc
     1921 gaggcagaac agcccgggga tcgtggtctg caggtggtag tcgacccaca ggccgcccat
     1981 cgtgtagtgc accgcggggt agatccgcat cgggaccttg tacggatcct cgccggtgat
     2041 ccgctcgtac atctcgaaca ggttgccgta cttctcgcgg acccggtcct cgccgtcgcg
     2101 ccggatcgcg tcgcggaagt cgaggtagac gccgcggccg ccgggcccga cgccgcggcc
     2161 ctcgtcgcac tgctccttgg cggcgcggct cgcgatgtcg cgcggcgaca ggttgccgaa
     2221 gctcgggtac ttgcgctcga ggtagtagtc gcgggcctcc tcggggatct ggtcggggtg
     2281 cctggcgacg tcgcctgccg acttcggcac ccagacccgg ccgtcgttgc gcagcgactc
     2341 ggacatcagg gtcagcttgg actggtagtc gccggcgacc gggatgcagg tcggatggat
     2401 ctgggtgaag cacgggttgg cgaacccggc gccgcgcttg taggcgcgat aggtcgcggt
     2461 gacgttgcag cccttggcgt tggtcgacag gtagaacgcg ttgccgtagc cgccggtcgc
     2521 gaggacgacg gcgtgcgccg agtgcgacga gatcgcgccc gtgatcaggt cgcgaacgac
     2581 gacgccgacc gcgcggccgt tgtcgacgac gatgtcgagc agctcggtgc gcgggaacat
     2641 cgtgaccttg ctgacaccga tctgacgcga cagcgccgcg tacacgccga gcaagagctg
     2701 ttgcccggtc tggccgcgcg cgtagaacgt ccgcgagacc tgggcgcggc cgaagctgcg
     2761 gttggcgagg gtgccgccgt actcgcgcgc gaacgggacg cccatggcgg cgcactggtc
     2821 gatgatgtcg accgagacct cggcgagacg gtgaacgttg gactcgcgcg cgcggaagtc
     2881 gccgcccttg acggtgtcgt agaacaggcg gtggatcgag tcgccgtcgt tctggtagtt
     2941 cttggcggcg ttgataccgc cctgtgcggc gatcgagtgg gcgcggcgcg gcgagtcctg
     3001 gaagcagaag cacgcgacgt cgtagccgag ctcggcgagc gtggcggcgg ccgcaccgcc
     3061 ggcgaggccc gagccgacga cgatgacctt gtacttgcgc ttgttggccg ggttcacgag
     3121 cgtcatcgcg aagcggtgat ggtcccactt ctgctcgatc ggaccgcccg ggatcctggc
     3181 gttcagctcc atgatgcgct ccgcgttgcc gctcccgccc cggtcacggc cggatcactc
     3241 cgaggtacac cgcgagcggg atgatcgtaa acccgcccca cacgagcacg gccaggacga
     3301 gcgccagctg ccggcggagc ggatgaggcg a
//
LOCUS       DASVOR010001765         2099 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_426668,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001765 DASVOR010000000
VERSION     DASVOR010001765.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_426668"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..642)
                     /locus_tag="VF516_32845"
     CDS             complement(127..642)
                     /locus_tag="VF516_32845"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112580.1"
                     /translation="MDTSDLDIDALLKRLHLANARRVWRDLVSRAEIEQWSCRDFLAL
                     LVAEEVAHRKQTRIQRVTSRCGFPFLKTVDDFDFTYQSTVKLSLLGSALSPDFVTDGR
                     SLILHGKPGRGKTHIAVAIGYRAIQNGFDTLFVTAAELIEDLSVASREGRLADALTRY
                     ITPHVLVCDSC"
     gene            complement(652..2061)
                     /gene="istA"
                     /locus_tag="VF516_32850"
     CDS             complement(652..2061)
                     /gene="istA"
                     /locus_tag="VF516_32850"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX8112581.1"
                     /translation="MVRRHEIQVLRRAGHSLDETAKLVGVSQSSVQRIATEPAVTTYD
                     TDAERARREVGRPSKAEPFRSFLVGELAVQPDVLSVELFRRAKLRGYAGGKSALYALI
                     KELRPERVRPVVRFEGLAGEFSQHDFGHVDVRYLDGRQRRVHFFASRLKYSRWVEVSL
                     VENERVEALVRAMVEHFAAWGGLPLLAVFDRPKTIALSWAKDGTVTEWNPTFGAVMLD
                     LGLGVELCWPRRPEQKGSVENLVGWVKGSFFKQRRFVDDDDLRRQLAEWHHEVNTQRP
                     SRATRIVPAVRLAEEQPRLRPLRVKPDELALRIPVSVGPTGYVVHDTHPYSMPPDALG
                     LPGTLYLYRDRVRIVAGRYQAEHERKFGRDEGSTLPEHRAQRVAAVSGRRAKRYMKRE
                     HLVGLGEHALAYITELVHRRPKVWIRDVDQLHEMLERYGDDALRAAFEIGLAHGAIGA
                     EYIHHYLTNTLGVVLGAVH"
BASE COUNT          345 a          665 c          759 g          330 t
ORIGIN      
        1 gaaacaactc gacgagcaca ggagcctcct tgggtggaag accacgaggc tacaggccga
       61 gttatgtgaa gtgatccaac cctgctgccg tcggagatcc tgccggtgct gcacataaca
      121 aggtgctcaa catgagtcgc agaccaagac gtgcggggtg atgtagcggg tgagcgcgtc
      181 ggcgagccga ccctcgcgcg aggcgacgga gaggtcctcg atgagctcgg cggcggtgac
      241 gaagagcgtg tcgaaaccgt tctggatcgc gcggtagccg atagcgacgg cgatgtgggt
      301 cttgccgcgg ccgggcttgc cgtggaggat cagcgagcgg ccgtcggtga cgaagtcggg
      361 cgacagcgcc gagccgagca gcgagagctt gacggtgctc tggtaggtga agtcgaaatc
      421 gtcgacggtc ttgaggaagg gaaagccgca gcggctggtc acgcgctgga tgcgggtctg
      481 cttgcggtga gcgacttcct cggcgacgag tagggccagg aagtcgcggc agctccattg
      541 ctcgatctcg gcgcgggaga cgaggtcgcg ccagacgcgg cgcgcgttgg cgaggtgcag
      601 acgcttgagc agcgcatcga tgtcgaggtc gctggtgtcc atggtgagct cctagtgcac
      661 ggcgcccagg acgacgccga gggtgttggt gaggtagtgg tggatgtact cggcgccgat
      721 cgcgccgtgg gccaggccga tctcgaacgc ggcgcggagc gcgtcgtcgc cgtagcgctc
      781 gagcatctcg tgcagctgat cgacgtcgcg gatccagacc ttgggccggc gatggaccaa
      841 ctcggtgatg tacgcgagcg catgctcgcc gaggccgacc aggtgctcgc gcttcatgta
      901 gcgcttggcg cgccgacccg agaccgcggc gacgcgctga gcgcgatgct cgggcaaggt
      961 ggagccttcg tcgcgaccaa acttgcgctc gtgctcggcc tgatagcggc ccgcgacgat
     1021 gcggacgcgg tcgcggtaca ggtacagcgt gccaggcagc ccgagcgcgt cgggcggcat
     1081 cgagtaggga tgcgtatcgt gcacgacgta gccggtcggc ccgaccgaca ccgggatccg
     1141 cagcgcgagc tcgtcgggct tgacccgcag cggacgaagc cgcggctgct cctcggcgag
     1201 ccgtacggcg ggcacgatgc gggtcgcgcg cgacggccgc tgcgtgttga cctcgtggtg
     1261 ccactcggca agctggcggc gcaggtcgtc atcgtcgacg aaacgccgct gcttgaagaa
     1321 cgagcccttg acccagccga cgaggttctc gaccgagccc ttctgctcgg ggcggcgtgg
     1381 ccagcacagt tcaacgccga ggccgaggtc gagcatcacc gcaccgaacg tcgggttcca
     1441 ctcggtgacg gtgccgtcct tggcccacga cagcgcgatg gtcttgggcc ggtcgaacac
     1501 ggcgagcagc ggcaggccgc cccaggcagc gaagtgctcg accatcgcgc ggaccagtgc
     1561 ttcgacccgc tcgttctcga ccaggctgac ctcgacccag cgcgagtact tcaggcgaga
     1621 cgcgaagaag tgcacgcggc gctgccgccc gtcgaggtac cgcacgtcga cgtggccgaa
     1681 gtcgtgctgc gagaactcgc cggcgaggcc ctcgaaccgg accaccggcc gaacgcgctc
     1741 ggggcgcagc tccttgatca gcgcgtacag cgcgctcttg ccaccggcgt agccccgcag
     1801 cttcgcccgg cggaacagct cgacggacag cacgtcgggc tggaccgcga gctctccgac
     1861 gaggaagctg cggaacggct cggccttgga aggtcgtcct acttcacgcc gagctcgttc
     1921 cgcatccgta tcgtaagtcg tgacggcggg ttcagtcgcg atgcgctgga cgctgctttg
     1981 cgacacgccg acgagcttgg ccgtctcgtc caggctgtgt cctgctcgac gcaggacctg
     2041 gatctcgtgc cttctgacca tgtcgatcat cctttgcctt gccgcgccgg ttggccacg
//
LOCUS       DASVOR010001766        10506 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_310756,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001766 DASVOR010000000
VERSION     DASVOR010001766.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10506
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_310756"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VF516_32855"
     CDS             <1..362
                     /locus_tag="VF516_32855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112582.1"
                     /translation="EPDRSPPAEPPFACPALGPDLTCTGGTHYRVASLAAGLVPIIAA
                     GRPAPVVVNGSITGLRLGAAVPGLGLQAGDVIVAVAGRLVTSRTMLADRIAHAGAATT
                     VTVRRGAEEAVLQFAER"
     gene            456..1724
                     /locus_tag="VF516_32860"
     CDS             456..1724
                     /locus_tag="VF516_32860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112583.1"
                     /translation="MGILAVACHGNDPAKGSAGSASSVSQTTPSAIASAGGKQPGGVH
                     VAEVKPPDIKPDTRGASAPPPPGGHDFTPDAKVLLSVGACGDLPPPPSFDPKLLAGHC
                     EIIKKAQDDYLSQWVRPAHGFFVEHVPSDIPRKVVYPFAGGDLSTALTVYPDADEITT
                     ISLEPAGDPRDLGELKGAELDRALKKVEYELKFLYRVNFSNTINMIDAMREGSLPTQL
                     IFGLSALKIHGFEVVGLRYFRLEPDGSIHYLDDAEVAAVPPPSKARATVRNRVFANAE
                     IQFRRPGGRTQVYRHIQWNLDNEHLKADPRILKHLEAKGSIAGMTKAASYLLSWDSFS
                     TMRDYLIDHVSWMVSDATGVAPKWGKPAGFEYETYGDFTGPHIQAGGSIAKDWRVEFE
                     SEPKRDLAFRFGYYDKHNANHLVIMRKKKA"
     gene            complement(1778..3244)
                     /locus_tag="VF516_32865"
     CDS             complement(1778..3244)
                     /locus_tag="VF516_32865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112584.1"
                     /translation="MATTCLLVPQGGCSLLLDSSQTQCTVDEDCAHFGDHPVCRSGVC
                     VMSGLGPPGCSTDPPTRPDQFANQCTTAVTLQFDNCSRLQLCNDSALATAMGTAMAPV
                     SLGTPPPPVNTESMPTVHCADAGNAIYVTGSTNLPPLIKAVQPLLYTGTPPHTVVFAP
                     QTSCKGAAAVYDPQPSKHLIFDTTNNWAFYYDRSGNQTFCLLDPAGNTVDVGESDVYP
                     QSCGYAPTVGIADYTGPIQAITFVVPAGSKQTSISAEAAHLVFGAGGNRNQAMPWTDP
                     RLYFIRSSGTGTVQLPSRAIGIDPGAWWGIDRLSAGNLVASLETVDPSQAEGAIGILS
                     SDFADRNRANLRVLAFQQHGQRYAYYPDSSADSFDKANVRDGHYPIWGAIHFLAATDN
                     AVPSEAARALITQFTVPKLDPTLVSAIIDAGFVPPCAMTVTHSSEIGPLSGFQPDFAC
                     GCFYDKKVTSATSCQSCTTSSDCTGAAKVCNYGFCEAQ"
     gene            3953..4942
                     /locus_tag="VF516_32870"
     CDS             3953..4942
                     /locus_tag="VF516_32870"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8112585.1"
                     /translation="MVAGMCPRCGGAVALHEGRPFVNAAGSVELWHRDCFEDRDALIT
                     RSDPLDFLPTPPTPRLVQVLGGGVAGSLLVAIVVGQWTWGEMAPPPPALLANVDPAPS
                     AEPVVLRVEAAAHERVPSRIVTVETELEARYEVPVRRGGRLDEMFPSLRAWTHPVTGS
                     AELMPEQGSRHFGARREGIDRPECGEGHCGIDLDGPRGRPIVAVAAGTVVRIELSELG
                     LDGRSGRYVRIEHDDGTFTSYMHMDDVADLRVGDRVAAGQYVGTLGATAVFSSAPHLH
                     FSLEVPDHPGLHGDITDTHYVDPAPFLVRSTIAEPPRSIAARPERRHAIKPAF"
     gene            5931..6917
                     /locus_tag="VF516_32875"
     CDS             5931..6917
                     /locus_tag="VF516_32875"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEX8112586.1"
                     /translation="MGAAKVAKVPNVARVPKIAKVAKVTKVDRSPYRKLLWVLSAFVG
                     VEVLVAIILTLLLVLPNQPDVVAGLSTRELVLIEAILFLSGLMSGLSGFGFSAVGAAT
                     LLFIPPVTEVPLLQTLSTGNQLLSLERLRADMPRAWRTFWAGPGWPIVGGMPGAYAGI
                     WMLGHLPAELLKAVFGSILVLYCVYSLFRSAGARLRGFDGPVTGVIVGFIGGAFGGFT
                     AFPGAALVVWTGLRDLPKAQSRAIVQPYIIMSQLFALGLIALQQPQWLTGRYFALLVC
                     TLPVVLPGTFCGVTLYRRISDVNFRRISFILLGASGAALLIKLYGAALVRLI"
     gene            complement(6937..8355)
                     /locus_tag="VF516_32880"
     CDS             complement(6937..8355)
                     /locus_tag="VF516_32880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEX8112587.1"
                     /translation="MPASETATSWVENLFQSSIVPTLVDYIKIPNKSMMFDPDWRAHG
                     YMDQATEMFAAWARTQLPAGATLEVVRLGERTPVIFIDIPGTGGRAGDTVMLYGHLDK
                     QPEMIGWRDGLGPWTPVLEGDKLYGRGSADDGYALFASLTAINVLRRDSIPHARCVVL
                     IEACEESGSVDLPAYIEHLAPRIGQLSLVICLDSGCANYDQLWSTTSLRGLVLGTLDV
                     SLITEGVHSGDGTGVIAASERIVRQLLDRLEDSHTGQIKLAELATEIPRQRIVQAERT
                     SQVIADETWSKFPLHTGVQPVTRDNVELILNRTWRPGLAITGAEGWPTIGNAGNVLRP
                     FTRLKLSIRIPPRVDPDRAAQAVKQLLEKDPPYGARVSFEISGATAGWDAPEMAPWLD
                     KALDAASLRHFGKPAMYMGEGGTIPFMYMLGRQFPRAQFCITGVLGPGSNAHGPNEFL
                     HLPTARKLTLCVADVLAEHARQ"
     gene            8486..8842
                     /locus_tag="VF516_32885"
     CDS             8486..8842
                     /locus_tag="VF516_32885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HEX8112588.1"
                     /translation="MVIRLADDKKPPAPPRREGERQTGLVLEERTRTKKPPMYKVLLH
                     NDDYTTREFVVWVLQTVFHKNENDAVAIMSHVHNHGIGVAGVYTFEVAETKVTKTMHL
                     AKAQQFPLQLSIEPTE"
     gene            8894..>10506
                     /locus_tag="VF516_32890"
     CDS             8894..>10506
                     /locus_tag="VF516_32890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8112589.1"
                     /translation="MLSPELKATLERAVEDVRSRHHEYLTLEHLLLSILDDPSGVDIV
                     VKCGGDVEKLRGDLERFLDEEVDELPDGDESGPDQTLAFQRVLQRAAMHVQAAGRQQM
                     TTGNVIASMFREKDSHAVYLLEKQGVSRFDVINYISHGVSKIESAGPVVPRSRGVEDD
                     GAEDQKVANPLESFCQDLTQRAREGKIDPLIGRADELERMIQVLSRRRKNNPLLIGEP
                     GVGKTALAEGLALRIVDGQVPQPLLDARVYSLDMTAVLAGTRYRGDFEERLKAVMKVL
                     IGDPKAILFIDEIHNIIGAGATSGGTMDAANILKPPLSNGELRCIGSTTFKDYRQAFE
                     RDRALSRRFQRIDIGEPTVPEAIEILKGLRPRYEDHHQVKYTDAAIESAVELSARHIS
                     GSHLPDKAIDVIDEAGARVRLMPAEQRPDEIRPMLIEEVVAKIARIPTRSVSSTDKKK
                     LADLETELKRLIFGQDKAIEQLATVIKLSRAGLGNPNKPTGNFLFAGPTGVGKTELAK
                     QLAAVLGVEFIRFDMSEYMEKHTVSRLIGA"
BASE COUNT         1619 a         3609 c         3630 g         1648 t
ORIGIN      
        1 ccgagcccga ccgcagcccc ccggccgaac cgccgttcgc gtgcccggcg ctcggtcccg
       61 acctcacctg caccggcggc acccactacc gcgtcgcctc gctcgccgcc ggcctggttc
      121 cgatcatcgc cgccggccgg cccgcgccgg tcgtggtcaa cggcagcatc accggcctgc
      181 ggctcggcgc ggcagtcccg ggcctcggcc tccaggccgg cgacgtgatc gtggcggtgg
      241 ccggccgcct cgtgacttct cgtaccatgc tcgccgaccg gatcgcgcac gccggcgctg
      301 cgaccaccgt caccgtccgg cgcggcgccg aggaggcggt gctgcagttc gccgagcgct
      361 gacgaatagc cttgagcagg agttcgttgc atgatgcagc tctgatgctt cgcaacaccc
      421 tgctcgctgc gtctatcgct gcgtctatcg ctgccttggg catcctggca gtcgcatgtc
      481 acggcaacga tcccgccaag gggagcgcgg gctccgcgtc gagcgtctcg caaaccaccc
      541 cgtcggcgat cgcatcggcg ggcgggaagc agcccggggg tgtccatgtc gccgaggtca
      601 agccgcccga tatcaagccc gataccaggg gcgcgtccgc accgcctccg cccggcggcc
      661 atgacttcac gcccgatgcc aaggtgctgc tctcggtcgg cgcgtgcggc gacctgccgc
      721 cgcccccgag cttcgatccc aagctgctcg ccggccactg cgagatcatc aagaaggccc
      781 aggacgacta cctctcccag tgggtccggc cggcgcacgg gttcttcgtc gagcacgtcc
      841 cgagcgacat ccccaggaag gtcgtctatc cgttcgccgg cggcgacctg tcgaccgcgc
      901 tcacggtcta ccccgacgcc gacgagatca ccacgatctc gctggagccc gccggtgatc
      961 cgcgcgacct cggcgagctc aagggcgccg agctcgaccg cgcgctcaag aaggtcgagt
     1021 acgagctcaa gttcctctac cgcgtcaact tcagcaacac gatcaacatg atcgacgcga
     1081 tgcgcgaggg ctcgctgccg acccagctga tcttcgggct gtccgcgctc aagatccacg
     1141 gcttcgaggt cgtcgggctg cgctacttcc ggctcgagcc cgacggctcg atccactacc
     1201 tcgatgacgc cgaggtcgcc gccgtgccgc cgccgtccaa ggcccgcgcc acggtccgca
     1261 accgcgtgtt cgccaacgcc gagatccagt tccgcaggcc gggcggccgg acccaggtct
     1321 accgccacat ccagtggaac ctcgacaacg agcacctcaa ggcggatccg cggatcctca
     1381 agcacctgga ggccaagggc tcgatcgccg gcatgaccaa ggcggcgagc tacctgctgt
     1441 cctgggactc gttctcgacc atgcgcgact acctgatcga ccacgtcagc tggatggtgt
     1501 ccgacgccac cggggtcgcc cccaagtggg gcaagccggc gggcttcgag tacgagacct
     1561 acggcgactt caccggcccg cacatccagg ccggcggctc gatcgccaag gactggcgcg
     1621 tcgagttcga gagcgagccc aagcgcgacc tcgcgttccg gttcggctac tacgacaagc
     1681 acaatgccaa ccacctcgtc atcatgcgca agaagaaggc ctgagccggc cctggcctct
     1741 ctggccggcg cgcccggcgt tgccacgcgg ccggcggcta ctgcgcttcg cagaatccgt
     1801 agttacagac cttggcggcg ccggtgcagt cgctggaggt ggtgcagctc tggcacgacg
     1861 tcgcgctggt caccttcttg tcgtagaagc agccgcaggc gaagtcgggc tggaaccccg
     1921 acagcgggcc gatctcggag ctgtgcgtca ccgtcattgc gcacggcggg acgaacccgg
     1981 cgtcgatgat cgccgagacc agcgtcgggt cgagcttggg cacggtgaac tgcgtgatca
     2041 gcgcgcgcgc ggcctcggac ggcaccgcgt tgtcggtggc cgccaggaag tggatcgcgc
     2101 cccagatcgg gtagtggccg tcgcggacgt tggccttgtc gaacgaatct gcgctcgagt
     2161 cggggtagta ggcgtagcgc tggccgtgct gctggaacgc cagcacgcgg aggttggcgc
     2221 ggttgcggtc ggcgaagtcg ctcgacagga tgccgatcgc gccctcggcc tggctcgggt
     2281 cgaccgtctc gagcgaggcg accaggttgc ccgccgatag ccggtcgatc ccccaccacg
     2341 cgccggggtc gatgccgatc gcccgcgacg gcagctgcac ggtgccggtg ccggagctgc
     2401 ggatgaagta gagccgcgga tcggtccacg gcatggcctg gttgcggttg ccgccggcgc
     2461 cgaacaccag gtgcgcggcc tccgcgctga tcgacgtctg cttcgatccg gccgggacga
     2521 cgaacgtgat cgcctggatc gggccggtgt agtcggcgat cccgaccgtg ggggcgtacc
     2581 cgcatgactg gggatagacg tcggactcgc cgacgtcgac cgtgttgccc gccggatcga
     2641 gcagacagaa cgtctggttg ccgctgcggt cgtagtagaa cgcccagttg ttggtcgtgt
     2701 cgaagatcag gtgcttgctc ggctgcgggt cgtagaccgc cgcggcgccc ttgcacgagg
     2761 tctggggcgc gaacaccacc gtgtggggcg gggtgccggt gtagagcagg ggctggaccg
     2821 ccttgatcag cggcggcagg ttggtcgacc ccgtgacgta gatggcgttg ccggcgtcgg
     2881 cgcagtgcac cgtgggcatc gactcggtgt tgaccggtgg gggtggtgtg ccgagcgaga
     2941 ccggcgccat cgccgtgccc atcgccgtgg ccagcgcgct gtcgttgcac agctggagcc
     3001 gcgagcagtt atcgaactgc agggtcacgg ccgtcgtgca ctggttggcg aactgatcgg
     3061 gccgggtcgg cggatcggtg gagcagccgg gtgggcccag cccggacatc acgcacaccc
     3121 cggatcgaca caccggatga tcgccgaaat gcgcacagtc ctcgtcgacg gtgcactgcg
     3181 tctgactgct gtcgaggagc agcgaacagc cgccctgcgg aacgagcaga caggtcgttg
     3241 ccaaagcaag gagcgctcgc gcaagaacgg gccgtaccag ctctcccata tcaatcgctg
     3301 tatcactgcg cgcgaagtca ttcaatagcg atctccgact gagatgtctt attttgatcc
     3361 ggagggtgtc tcgcagcgat ccgctgcgcc ggacggccgg cgctcgacga gcgccggtgt
     3421 caacgcgaac gcgaccgtcg cagcgcgccg gcgctcaccg tgccgcacgg agcaaatgac
     3481 tgcactctgc gcacaggcaa acaatgtatt cctgtccgcg aaacatggtt gtcgtccggg
     3541 tgatgtcctg tgggcgcaca gtgcctcgct cgccgcggat cggcatcccc gtacgtgggc
     3601 aggcccggat ccagcgcagg gtggtgcagc agaacgcatc ggggcgcgct gcgcgacgcg
     3661 aacgtcgatc tcccgcagcg tgcgtcgcgc cggcagtcca ccatcgccgc gcgcgtcctc
     3721 agcagttctc tcaggtcctc ggacggcgct tcgctgccga tcgcagggcc cgatcgacga
     3781 tcgcgcgcga gaacgggccg cgtgcagcgg gggtgcgctg cctcgggggc tgcggtgtca
     3841 cagccggtgt gaaaaaatac gatcgatctc cccgggtgta gggcgtgttg cgacagctgg
     3901 aatcctggtt ttgtcgagcg atctcacccg cctcttgcgt tacggtgagc ccatggtggc
     3961 cggaatgtgt ccgcgatgtg gaggcgcggt cgcattgcac gagggacgcc cgttcgtcaa
     4021 cgcggcgggc tccgtcgagc tatggcatcg cgactgcttt gaagaccgcg acgcgctgat
     4081 cacgcggtcc gacccgctcg acttcctgcc gaccccaccg accccccggc tggtgcaggt
     4141 gctcggcggg ggggtggccg gctcgctgct ggtcgcgatc gtggtcgggc agtggacctg
     4201 gggcgagatg gccccgccgc cgcccgcgct gctcgccaac gtcgatcccg cgcccagcgc
     4261 ggagccggtc gtgctccggg tggaggccgc cgcgcacgag cgggtgccgt cccggatcgt
     4321 cacggtcgag accgagctcg aggcgcgcta cgaggtgccg gtgcgccggg gggggcggct
     4381 cgacgagatg ttcccgtcgc tgcgcgcctg gacccatccg gtgaccggca gcgccgagct
     4441 gatgcccgag caggggagcc gccatttcgg cgcgcggcgc gagggcatcg accggccgga
     4501 gtgcggcgag ggccactgcg gcatcgatct cgacggcccg cgcggccggc cgatcgtcgc
     4561 ggtcgccgcc ggcacggtgg tccgcatcga gctcagcgag ctcgggctcg acggccgcag
     4621 cggccgctac gtccggatcg agcacgacga cggcacgttc acgtcgtaca tgcacatgga
     4681 cgacgtcgcc gacctccgcg tcggcgatcg ggtcgccgcc ggccagtacg tcggcacgct
     4741 gggggccacc gcggtgttct cgtcggcgcc gcacctccac ttcagcctcg aggtcccgga
     4801 ccacccgggc ctgcacggcg acatcaccga cacccactac gtcgacccgg cgccgttcct
     4861 ggtccgctcg acgatcgccg agccaccgcg ctcgatcgcc gcgcggcccg agcggcggca
     4921 cgcgatcaag cccgcgttct gaagaggcgt agaaattgag ctcgcgctga gtcaacgacg
     4981 agatctgatg attcgtcaca agttggggga catggggttg acctgaacgt cgaccttgac
     5041 ccctccgttg aggttgacgg cagcgaagcg atacgtagac gtctgacccc accgtcgacc
     5101 tcgacccacc cccgttgtcg cgctccgtcg aggctcgcgg cacaggatca gtcgacgtcc
     5161 aacgtcgatg gtgggctcaa cgtcgtacgt cgccgtcgag ctcaacgctc tgggtcaacg
     5221 atcaggtcga cgtcgacgat cccggacgcg ggccgagaat ccgagcgtca ttcacatctg
     5281 catcccatcg ccatgtggat gcacgccgcg ggcactgcgc acgggcatgc acatcgcgga
     5341 tgccggcggc cgtctggcgc cggcatccgc ccgttcgtga gagaccgctg acctcgtcga
     5401 ccagcaggcc gcctcgcgct gtgcacacct gtgaggctgc gcacgtcctg gctcaagtct
     5461 ctcgaggacc gcggcaaatg gccccgccga ccgcgggtcg aaggcggcta gtgccgggtg
     5521 ggccgcaagc gcgcttaccg gggctaccgt ccgcgaggtc gatctgcacc cggcgaggag
     5581 caaccgagca cccatcatcg cacatcgctc gagcgatgca tttctggaac gcgcggctgg
     5641 acgttgcgat cgcggtcgcc gatactgata tcggcgggtc ttctggccgg tgtcggtcgt
     5701 cacgatacct aacgctcccc tccacatccc ctcgtatcgc gacctgcagg cagtcgatgc
     5761 agtgactgcg gtattccgga ctcccccgga tcaataccgc attcgatggt gcgtgatttt
     5821 gcgtggatcg gcgagattat gagcgcagcc ttacccgtag ctcggctata gtcatttcca
     5881 ccgacggaaa ttgagaactg cctgcaggag gtccggacat ggtaactgcc gtgggagctg
     5941 caaaggtcgc caaggtcccg aacgtcgcaa gggtccccaa gatcgccaag gtcgccaagg
     6001 tcacgaaggt agaccgctcg ccgtatcgca agctcctgtg ggttctgtcc gcgttcgtgg
     6061 gcgtggaggt cctggttgcc atcatcctca cgctcctgct ggtgctcccc aaccagcctg
     6121 acgtggtcgc cggcctcagc acccgcgagc tcgtgctgat cgaggcgatc ctgttcctgt
     6181 ccggtctcat gagcgggctc tcggggttcg ggttctccgc cgtgggcgcg gccacgctgc
     6241 tgttcatccc gccggtcacc gaggtgccgc tgctgcagac gctgtcgacc gggaaccagc
     6301 tgctgtcgct ggagcggctg cgcgccgaca tgccgcgcgc gtggcggacg ttctgggcgg
     6361 ggcccggctg gccgatcgtc ggcggcatgc cgggcgcgta tgccgggatc tggatgctcg
     6421 gccacctgcc ggccgagctg ctgaaggcgg tgttcggatc catcctggtc ctgtactgcg
     6481 tgtactcgct gttccggtcg gccggtgccc ggctgcgcgg cttcgacggc ccggtcaccg
     6541 gcgtgatcgt cggcttcatc ggcggggcgt tcggcggctt caccgcgttc cccggcgccg
     6601 cgctggtggt gtggaccggc ctgcgcgacc tgcccaaggc gcagagccgg gcgatcgtgc
     6661 agccgtacat catcatgtcg cagctgttcg cgctcggcct gatcgcgctc cagcagccgc
     6721 agtggctgac cggccgctac ttcgccctgc tggtgtgcac gctgccggtc gtgctgccgg
     6781 gcacgttctg cggggtcacg ctctaccgcc ggatctccga cgtcaacttt cgccggatct
     6841 cgttcatcct gctcggggcg tcgggtgcgg ccttgctgat caagctctac ggcgcggcgc
     6901 tcgtgcggct gatctgaccg ccgtccctgg ccgccgtcac tgccgggcgt gctcggcgag
     6961 cacgtcggcg acgcacagcg tgagcttgcg cgcggtcggc aggtggagga actcgttggg
     7021 gccatgcgcg ttcgagccgg ggccgagcac gccggtgatg cagaactgcg ccctggggaa
     7081 ctgccggccg agcatgtaca tgaacgggat cgtgccgccc tcgcccatgt acatcgcggg
     7141 cttgccgaag tggcggagcg acgcggcgtc gagcgccttg tcgagccacg gcgccatctc
     7201 cggcgcgtcc cacccggcgg tggcgccgct gatctcgaac gacacccggg cgccgtacgg
     7261 cggatccttc tcgagcagct gcttgacggc ctgggccgcc cggtcgggat cgacgcgggg
     7321 cgggatccgg atcgacagct tgagccgggt gaacgggcgc agcacgttgc cggcgttgcc
     7381 gatcgtcggc cagccctcgg cgccggtgat cgccagcccg gggcgccagg tccggttgag
     7441 gatcagctcg acgttgtcac gcgtgaccgg ctggacaccg gtgtggagcg ggaacttgct
     7501 ccaggtctcg tcggcgatga cctgcgaggt gcgctcggcc tgcacgatgc gctggcgcgg
     7561 gatctcggtc gcgagctcgg cgagcttgat ctgcccggtg tggctgtcct ccagccggtc
     7621 gagcagctgg cgcacgatgc gctcgctcgc cgcgatcacc ccggtgccgt cgccggagtg
     7681 gacgccctcg gtgatcagcg agacgtcgag cgtgccgagc accaggccgc gcagcgaggt
     7741 ggtcgaccac agctggtcgt agttcgcgca ccccgagtcg aggcagatca ccagcgacag
     7801 ctggccgatc cgcggcgcga ggtgctcgat gtacgccggc aggtcgacgc tgccgctctc
     7861 ctcgcaggcc tcgatcagca ccacgcagcg cgcgtgcggg atgctgtcgc ggcgcagcac
     7921 gttgatcgcg gtgagcgacg cgaacagcgc gtagccgtcg tcggcgctgc cccggccgta
     7981 cagcttgtcg ccctccagca ccggcgtcca cggtccgagg ccgtcgcgcc agccgatcat
     8041 ctcgggctgc ttgtcgaggt ggccgtacag catgaccgtg tcgccggcgc gcccgccggt
     8101 gccggggatg tcgatgaaga tcaccggggt gcgctcgccg agccggacca cctcgagggt
     8161 cgcgccggcg ggcagctggg tcctcgccca cgccgcgaac atctcggtcg cctggtccat
     8221 gtagccatgc gcccgccagt cggggtcgaa catcatcgac ttgttgggga tcttgatgta
     8281 gtcgacgagc gtcgggacga tcgacgactg gaacaggttc tcgacccacg aggtggcggt
     8341 ttcggaggcg ggcatggcgg gactctactt tcgatcgggg gggtggatcc agggcgtgaa
     8401 ctccgcgaaa cctgcccgaa attgcgggtg caacgcgcgg ccccgagcgc tacacaccgg
     8461 ggcgagctct cgtactattg gaggcatggt gatccggctt gcggacgaca agaaaccccc
     8521 ggcgccgccc cgccgcgagg gcgagcggca gaccggcctg gtgctcgaag agcggacccg
     8581 caccaagaag cctccgatgt acaaggtcct cctgcacaac gacgactaca cgacccgcga
     8641 gttcgtggtc tgggtgttgc agaccgtgtt ccacaagaac gagaacgacg cggtcgcgat
     8701 catgagccac gtccacaacc atggcatcgg ggtcgcgggc gtctacacgt tcgaggtcgc
     8761 cgagaccaag gtgaccaaga ccatgcacct ggccaaggcc cagcagttcc cgctccagct
     8821 ctcgatcgag cccacggagt gacggggacc catggtggcc gcgaccgaaa tgtacggaac
     8881 gaaaaggtga tgcatgcttt ctcccgagct caaagccacg ctggagcgcg cagtcgagga
     8941 cgtccgcagc cggcaccacg agtacctgac cctcgagcac ctgctgctgt cgatcctgga
     9001 cgatcccagc ggggtcgaca tcgtggtcaa gtgcggcggc gacgtcgaga agctgcgcgg
     9061 cgatctggag cggttcctgg acgaggaggt cgacgagctg cccgacggcg acgagtccgg
     9121 ccccgaccag accctggcgt tccagcgcgt gctgcagcgc gcggcgatgc acgtccaggc
     9181 cgccgggcgc cagcagatga ccaccggcaa cgtgatcgcg tcgatgttcc gcgagaagga
     9241 ctcgcacgcg gtctacctgc tggagaagca gggcgtgtcg cggttcgacg tcatcaacta
     9301 catctcgcac ggcgtgtcga agatcgagtc ggccggcccg gtcgtgccgc ggtcgcgcgg
     9361 cgtcgaggac gacggcgccg aggaccagaa ggtcgccaac ccgctggaga gcttctgcca
     9421 ggacctcacc cagcgggccc gcgagggcaa gatcgacccg ctgatcggcc gggccgacga
     9481 gctcgagcgc atgatccagg tgctgtcgcg gcggcgcaag aacaacccgc tcttgatcgg
     9541 cgagcccggg gtcggcaaga ccgcgctggc cgagggcctc gcgctgcgga tcgtcgacgg
     9601 gcaggtgccg cagccgctgc tcgacgcccg ggtctactcg ctcgacatga ccgcggtgct
     9661 cgccggcacg cgctaccgcg gcgacttcga ggagcggctc aaggcggtga tgaaggtcct
     9721 gatcggcgat cccaaggcga tcctgttcat cgacgagatc cacaacatca tcggcgccgg
     9781 cgcgaccagc ggcggcacga tggacgcggc caacatcctc aagccgccgc tgtccaacgg
     9841 cgagctgcgc tgcatcggct cgaccacgtt caaggactac cgccaggcgt tcgagcgcga
     9901 ccgcgcgctg tcgcggcggt tccagcgcat cgacatcggt gagccgacgg tgcccgaggc
     9961 gatcgagatc ctgaagggcc tgaggccgcg ctacgaggac caccaccagg tcaagtacac
    10021 cgacgccgcg atcgagtccg cggtcgagct gtcggcgcgc cacatcagcg gctcgcacct
    10081 gcccgacaag gcgatcgacg tgatcgacga ggcgggagcc cgggtccggc tgatgcccgc
    10141 cgagcagcgc cccgacgaga tccgcccgat gctgatcgag gaggtggtgg ccaagatcgc
    10201 gcggatcccg acccgctcgg tgtcgtcgac cgacaagaag aagctcgccg acctcgagac
    10261 cgagctcaag cggctgatct tcggccagga caaggcgatc gagcagctcg cgacggtgat
    10321 caagctgtcg cgcgccggcc tgggcaaccc gaacaagccg accggcaact tcctgttcgc
    10381 cggcccgacc ggcgtcggca agaccgagct cgccaagcag ctcgccgcgg tgctcggcgt
    10441 cgagttcatc cggttcgaca tgtcggagta catggagaag cacacggtgt cgcggctgat
    10501 cggcgc
//
LOCUS       DASVOR010001767         3973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_222825,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001767 DASVOR010000000
VERSION     DASVOR010001767.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222825"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3973
                     /locus_tag="VF516_32895"
     CDS             <1..>3973
                     /locus_tag="VF516_32895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112590.1"
                     /translation="SDQQAAQAAAQQQTDAANARANAEAPAQMRAAAAPAQQQLTSQI
                     ASTQSTATQSATAQMAQHQSRAQQIVQSQPPITGAQAAAQAQPQYQAHQAEATQASQQ
                     MATSITTAQTTATTQASAARTQRDQHINQAQQGYQQTVSGQLMPQYAQQQQQAHTSFT
                     ASDQAAQQQQASDQQTAQAAAQQQTDAANAKANADIASHQQQLSSQQQAAAASHDQQV
                     AAANAQHDQQMQQAQQQSDQQIQQHQADMQSQVSQRQAQGNAEVNQHLSEGEQGYHQE
                     IAKGQSDADAAKAKAEADAAAKKQQADAEKSKHHGGGLFGSIVGWVEDRVNDLLDAAK
                     AVLEAAKNLVISIMEKARQAAMAVLEKARQAALAALNAVKSAIQGIISACAAAIRSVI
                     SACAAMIRGLIQALADVLKGLVQALTTLLTTLVQAFQAAVNAVLDGLIAAVSLVNKDL
                     GNKLRAATQKYRDAFNNACNKLQSDIKAAGNALQAGIQKAADAAISVVNAAEQTLDNA
                     VTAVEHTLNAAVEAAYQLGAKAINTAFDAAEAVVNKAFDVAEAAVKAYFDAQIGALGL
                     VQKAVDAVGDFVVEVANKALEALDKVAQAIVNAIPDSWTKALVDFWNGPWRTAIIIGL
                     ATIAAVAITAATLGTGAPIAAMLMAGVIGGAIAGTAYFGGEMLAREGEIKLSSEGDGM
                     YIPGYGKVQIGPDGKPIPPPGLSPEKMADFEKQSQWAVSNFNLQRDDNGQVVGYDRKS
                     GLDIADDAVKEGVKGFGEGFISSALAVAGGGVGGALTKSIGNEFLKAGLESGITNVIT
                     GPIQTSLTAGWDKGFDALASGKNPLDAMKEAWGGMKEQLTNPGNWAGALLMTGIAPAK
                     LKFITPKLNSALEGVENNAVKTVVTKGTDVVVETMGMTFGTAGGNFVGGVYGALKDGK
                     SLNDALDAGRKAADEAFQPEAILTTAAISTAGQIAHPGGEHPGQHEGGTSEPHPTSEP
                     HPTSEPRAATPEADHQLQQQIERVTGVDKGPEHEQAMADLRDYYTQQNLEGQNTIQVS
                     GTRTEGPYSWTFGHDATEFSNGTTHVEYRVYLDGTAHNIPPEQMAQLRDNIVSGVDQH
                     YNYQHEITSSSGDPSRLHMEVVFVDDPAQAHVQVEVHPGDGPANLANWYVDGSPTTHA
                     HEIGHGAFGFLDEYHDPSAPDRATPTSPGVHDDGSLMGNYWEVQPDGSYAPAAGTGLQ
                     DRHIDEISALHDQNAPTGPLAAPPPAPEHPGVPTHELENQLAAIDHTQAGTPEQLAVV
                     REARGEIATLQAMRDLVASGGGPWEHATPAQREQQIKLIDDQIRSTAETALDQHRQGQ
                     PELA"
BASE COUNT          768 a         1477 c         1348 g          380 t
ORIGIN      
        1 cgagcgacca gcaggccgcc caggccgccg cgcagcagca gaccgacgcg gccaacgcca
       61 gggccaacgc cgaggcgccg gcccagatgc gcgccgccgc ggcgccggcg cagcagcagc
      121 tgacctcgca gatcgcgagc acgcagagca ccgcgaccca gagcgcgacg gcgcagatgg
      181 cgcagcacca gagccgcgcc cagcagatcg tccagagcca gccgccgatc accggcgcgc
      241 aggccgccgc ccaggcgcag ccgcagtacc aggcgcatca ggccgaggcg acgcaggcca
      301 gccagcagat ggccacgtcg atcacgacgg cgcagaccac ggccacgacc caggccagcg
      361 cggcgcgcac ccagcgcgac cagcatatca accaggcgca gcagggctat cagcagaccg
      421 tctccggcca gctgatgccg cagtacgcgc agcagcagca gcaggcccac acgtcgttca
      481 cggccagcga tcaggcggcg cagcagcagc aggcgagcga ccagcagacc gcccaggccg
      541 ccgcgcagca gcagaccgac gcggccaacg ccaaggccaa cgccgatatc gcctcgcacc
      601 agcagcagct gagctcccag cagcaggcgg cggctgcgag ccacgatcag caggtcgcag
      661 cggccaacgc gcagcacgac cagcagatgc agcaggccca gcagcagtcc gaccagcaga
      721 tccagcagca ccaggccgac atgcagagcc aggtgtcgca gcgccaggcg cagggcaacg
      781 ccgaggtcaa ccagcacctc agcgagggcg agcagggcta ccaccaggag atcgcgaagg
      841 gccagtccga tgccgacgcc gccaaggcca aggccgaggc cgacgcggcc gccaagaagc
      901 agcaggccga cgccgagaag agcaagcacc acggcggcgg cctgttcggc tcgatcgtcg
      961 gctgggtcga ggaccgggtc aacgatctgc tcgacgccgc caaggccgtc ctcgaggccg
     1021 ccaagaacct ggtcatctcg atcatggaga aggcgcgcca ggcggcgatg gcggtgctcg
     1081 agaaggcgcg ccaggccgcg ctcgccgcgc tcaacgcggt caagtcggcg atccagggca
     1141 tcatctcggc gtgcgccgcc gcgatccgct cggtgatcag cgcgtgcgcg gcgatgatcc
     1201 gcgggctgat ccaggccctg gccgacgtgc tcaagggcct ggtccaggcg ctgaccacgc
     1261 tgctcaccac gctggtccag gcgttccagg ccgcggtcaa cgcggtgctc gacggcctga
     1321 tcgcggcggt cagcctggtc aacaaggacc tcggcaacaa gctgcgggcc gcgacccaga
     1381 agtaccgcga cgcgttcaac aacgcgtgca acaagctgca gagcgacatc aaggccgccg
     1441 gcaacgcgct gcaggccggg atccagaagg ccgcggacgc ggcgatctcg gtggtcaacg
     1501 ccgccgagca gacgctcgac aacgcggtca ccgcggtcga gcacacgctc aacgcggcgg
     1561 tcgaggccgc gtaccagctc ggcgccaagg cgatcaacac cgcgttcgac gccgccgagg
     1621 cggtggtcaa caaggcgttc gacgtcgccg aggcggcggt caaggcctac ttcgacgcgc
     1681 agatcggcgc gctcggcctg gtccagaagg cggtcgacgc ggtcggcgac ttcgtggtcg
     1741 aggtcgccaa caaggcgctc gaggcgctcg acaaggtcgc ccaggcgatc gtcaacgcga
     1801 tcccggactc gtggaccaag gcgctggtcg acttctggaa cgggccgtgg cggaccgcga
     1861 tcatcatcgg cctggcgacg atcgccgccg tcgcgatcac cgccgcgacg ctcggcaccg
     1921 gcgcgccgat cgcggcgatg ctgatggcgg gcgtgatcgg cggcgcgatc gccggcaccg
     1981 cgtacttcgg cggcgagatg ctcgcgcgcg agggcgagat caagctcagc tccgagggcg
     2041 acgggatgta catcccgggc tacggcaagg tccagatcgg ccccgacggc aagccgatcc
     2101 cgcctcccgg cctcagcccc gagaagatgg ccgacttcga gaagcagagc cagtgggcgg
     2161 tgagcaactt caacctccag cgcgacgaca acggccaggt cgtcggctac gaccgcaaga
     2221 gcggcctcga catcgccgac gacgcggtca aggagggcgt caagggcttc ggcgagggct
     2281 tcatctcgag cgcgctggcg gtcgccggcg gcggcgtcgg cggcgcgctg accaagagca
     2341 tcggcaacga gttcctcaag gccgggctcg agtccggcat caccaacgtc atcaccggcc
     2401 cgatccagac ctcgctgacc gcggggtggg acaaggggtt cgacgcgctc gccagcggca
     2461 agaacccgct cgacgcgatg aaggaggcgt ggggcgggat gaaggagcag ctgaccaacc
     2521 cgggcaactg ggccggcgcg ctcctcatga ccggcatcgc gccggccaag ctcaagttca
     2581 tcacccccaa gctcaacagc gcgctcgagg gtgtcgagaa caacgcggtc aagaccgtcg
     2641 tcaccaaggg caccgacgtc gtggtcgaga ccatgggcat gacgttcggc accgccggcg
     2701 gcaacttcgt cggcggcgtc tacggggcgc tcaaggacgg caagtcgctg aacgacgcgc
     2761 tcgacgccgg ccgcaaggcc gccgacgagg cgttccagcc cgaggcgatc ctgaccaccg
     2821 cggcgatcag caccgcgggt cagatcgccc accccggcgg cgagcatccc ggccagcacg
     2881 agggcggcac gagcgagccg cacccgacca gcgagccgca cccgaccagc gagccgcgcg
     2941 cggccacgcc cgaggccgat caccagctcc agcagcagat cgagcgcgtc accggcgtcg
     3001 acaaggggcc cgagcacgag caggcgatgg ccgacctgcg cgactactac acccagcaga
     3061 acctcgaggg ccagaacacg atccaggtca gcgggacccg gaccgaaggc ccgtacagct
     3121 ggacgttcgg ccacgacgcg accgagttct ccaacggcac gacccacgtc gagtaccggg
     3181 tctacctcga cggcacggcc cacaacatcc cgcccgagca gatggcgcag ctgcgcgaca
     3241 acatcgtctc gggcgtcgac cagcactaca actaccagca cgagatcacc agctccagcg
     3301 gcgacccgag ccggctgcac atggaggtcg tgttcgtcga cgatccggcc caggcccacg
     3361 tccaggtcga ggtccatccc ggcgacggcc cggccaacct ggcgaactgg tacgtcgacg
     3421 gctcgccgac cacgcacgcc cacgagatcg gccacggcgc gttcggcttc ctcgacgagt
     3481 accacgatcc cagcgcgccc gaccgcgcca ccccgaccag ccccggcgtc cacgacgacg
     3541 gcagcctgat gggcaattac tgggaggtcc agccagacgg ctcgtacgcc ccggcagcag
     3601 gcaccggcct gcaggaccgc cacatcgacg agatctcggc gctgcacgac cagaacgcgc
     3661 cgaccggccc gctcgccgcg ccgcccccgg cgcccgagca ccccggcgtg ccgacccacg
     3721 agctcgagaa ccagctcgcg gcgatcgatc acacgcaggc cggcacgccg gagcagctcg
     3781 ccgtggtgcg cgaggcgcgc ggcgagatcg ccacgctcca ggcgatgcgc gacctcgtcg
     3841 ccagcggcgg cggaccgtgg gagcacgcga cccccgcgca gcgcgagcag cagatcaagc
     3901 tgatcgacga ccagatccgc agcaccgccg agaccgcgct cgatcagcat cgccagggcc
     3961 agcccgagct cgc
//
LOCUS       DASVOR010001768         5376 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_544415,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001768 DASVOR010000000
VERSION     DASVOR010001768.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5376
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544415"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="VF516_32900"
     CDS             <1..1203
                     /locus_tag="VF516_32900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX8112591.1"
                     /translation="IARDITERKRIERELAYYIAELSAADLRKNEFLAMLGHELRNPL
                     SALAHGLALQGNVPDDHARSEELREMMVRQTQRMGTLLDQLLDVARITSGKVELSSDR
                     VDLSEVVQVAVETVSSLVETEHHALTLALPSERVFVLGDAVRLAQVVENLLTNAVKYT
                     DQAGQIRVAIEASRNEARISVFDTGIGMTSELLPHVFEVFTQAPRALDRAKGGLGLGL
                     PLARRLVEMHGGHIDATSPGVGQGSEFIVTLPRLLERPSKELPSPANSAPGPTLPAAA
                     RSRRILVVDDQEESRELLAELLEVHGHRALAVNGGPAALAAIRTFGPEVVLLDIGLPE
                     MDGYEVARRLRDDYPNTSMLLIAVTGYQSDAARLKQAGFDEHLIKPPDMHKLFAMLAE
                     PDAGTGAR"
     gene            complement(1259..2347)
                     /locus_tag="VF516_32905"
     CDS             complement(1259..2347)
                     /locus_tag="VF516_32905"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112592.1"
                     /translation="MPDNQKAKDRDATDESLGEERRKTDDELALRRADIEDDADAVVA
                     NARAKADEVVRATREAADLRSPEPDEIGRLAAERRQDDDLLAGEREVVDRQRNIERAD
                     RHRVIAALLETERQSTDASLLSERRSADSAITARDDFLGMVSHDLRTLIAGIGLSAAM
                     LIHEAPPDDAGQRVVQRADAIQRFTTRMTRLIGDLLDVTSIEAGRLGLVVAKLDASRL
                     LTETLDAFQQVAFARGIALTVDMAGDALLARFDHDRILQVLANLVGNAIKFTEREGRI
                     DIAVQRQGDEVLFSVRDTGPGIAHKDLAAIFDRFWQAPTKTRRGLGLGLYISKCIIES
                     HGGRIWAESELGRGSVFHFTLPSGSSVS"
     gene            complement(2794..2961)
                     /locus_tag="VF516_32910"
     CDS             complement(2794..2961)
                     /locus_tag="VF516_32910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112593.1"
                     /translation="MKKITRKLVVRSETVRTLDNIDLVRAKGGSAPAVGGDRTQSGMD
                     CIAQAAVVVPK"
     gene            complement(2958..4985)
                     /locus_tag="VF516_32915"
     CDS             complement(2958..4985)
                     /locus_tag="VF516_32915"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8112594.1"
                     /translation="MWSAAFSPDGRQIVTTDDRAAQVWIAQTYRRMFTLDHGDIVYHA
                     VYSADGTKLVTGCGDGAVRSWDPATGVLLRELRHDTVKPRYRVVALSPDGKLVVGLDY
                     RMAHVWNIGTGMLVADLRVTAPSTYPSLAFSSDGRWLAMGTGNDVDVLDTQTWTVAHN
                     ISGLSIHSLSWDPTGSRLVTGSAEGDASIWKLPSGGRVQRLRELGEPIDTVAFSPDGR
                     LIAAGSRDGAVQVWDATSAKLRSQSNYLRSEVLSVEFDRASSLIAASGSNGAVAVSEA
                     ASGMPVTGLDGPATKVRVAHFDPTSRRVVGASWDGTVRIWDATAPYRRWSSPFTDNCG
                     LVTSLEPDRRFLAITCNDQPTRIWDTAHDHLVAELPSVTLVEGDFTSAYPAVSLAGDR
                     AAIARGNNVEVYELPAARLLSTIRHGAPVNAVAFAATGRDIVSGAIDGSIIVTRDNGA
                     MIALPRSSSGIDAAGFLADGRVIVADAGGRLRVYDPGGTTLAELHTLARVAMLRMSPD
                     SRRLITVSRFTEKVVAPELWDMELYRPVAQLAASGQGQVYSARFVARDDVITACGDGA
                     VRLWGAETGELHRTYRGGSRFLVDAKLSADGTMLIGGGGDGQLRFWEAASGRPLWTMP
                     AHRSHLIGVHVEGADIVTRGFLGDIARWSLPKPEQVLEACDHDERCAIVTQ"
     gene            5070..>5376
                     /locus_tag="VF516_32920"
     CDS             5070..>5376
                     /locus_tag="VF516_32920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112595.1"
                     /translation="MVAALVGAAEVDRRLELAVEQRRSPCSSSDSVMVASVTRRSARA
                     CVTVRYRNPPSTVTAASATSARLAKRLRRMRRWLAATNERSSGPIVCWGTLRRVSSST
                     "
BASE COUNT          944 a         1808 c         1694 g          930 t
ORIGIN      
        1 atcgcccggg acatcaccga gcgaaagcgg atcgaacgcg agcttgcgta ctacatcgcc
       61 gagctatccg cggccgatct ccgcaagaac gagtttctgg cgatgcttgg tcacgagctt
      121 cgcaatccgc tgtccgcgct cgcccacggc ctcgcgttac agggcaacgt gccggacgat
      181 cacgcgcggt ccgaggaact gcgcgagatg atggtcaggc agacccagcg catgggcacc
      241 ctgctcgatc agctgctcga cgtcgcgcgc atcacctcgg ggaaagtcga gctgtcgagc
      301 gaccgcgtcg acctgtcgga agttgtccaa gttgcggtcg aaacggtgag ctcgctcgtg
      361 gagaccgaac accacgcgct cacgctggcg ctgccctccg agcgcgtgtt cgtgctgggc
      421 gatgcggttc ggctggccca ggtcgtcgag aaccttctga cgaacgcggt caagtacacc
      481 gaccaagccg gacagatccg agtggcgatc gaagcgtctc gcaacgaggc ccggatctcg
      541 gttttcgata ccgggatcgg gatgacctcc gagctccttc ctcacgtctt cgaggtcttc
      601 acccaggcgc cgcgtgcgct ggatcgtgca aaaggtgggc tcgggctcgg tctgcccctc
      661 gcgcgtcggc tcgtcgagat gcacggcgga cacatcgacg cgacaagccc tggtgtcggg
      721 cagggcagcg agttcatcgt cacgctgcct cgactgctcg aaagaccttc gaaagaactg
      781 cccagccccg cgaacagcgc acctggcccg acgctccccg cggcggctcg gtctcgccgg
      841 atcctcgtcg tggacgacca ggaggagagc agagagcttc tcgccgagct tctcgaggtg
      901 cacggtcaca gggcccttgc cgtcaacggc ggcccggccg cattggcggc catcagaacg
      961 tttggtcccg aggtcgtgct cctggacatc gggttaccgg agatggacgg gtacgaagtc
     1021 gcgagacggc ttcgcgacga ctaccccaac acgagcatgc tgctgatcgc cgtgacgggc
     1081 taccagagcg atgccgctcg gctgaagcag gccggctttg acgagcacct gatcaagccg
     1141 cccgacatgc acaagctctt cgccatgctc gcggaaccgg acgcaggcac tggtgccagg
     1201 tagctccgat cggccagagc tagttctagc ctagcaacca ccaatcgtcg ccagtcgatc
     1261 acgacacaga cgagccgcta ggcaacgtga aatggaagac gctgccacgt cccaactcgc
     1321 tttctgccca gatccggcca ccgtgcgact cgatgataca cttcgagatg tagaggccga
     1381 gcccaagacc gcgccgggtt ttcgtcggag cctgccagaa ccgatcgaag atcgcggcca
     1441 ggtctttgtg ggcgatgccc ggccctgtgt cgcgaaccga gaacaacacc tcgtccccct
     1501 gacgctgcac ggcgatatcg atccggccct ctcgttcggt gaacttgatg gcgttgccaa
     1561 cgaggttcgc gagcacttga aggatgcgat cgtgatcaaa cctcgccagc agtgcgtcgc
     1621 cggccatgtc cacggtgagc gcgatgccgc gcgcgaaggc tacctgttga aacgcgtcca
     1681 aggtctccgt cagcaaacgg gaagcgtcga gcttcgcgac gacgaggcca agccggcccg
     1741 cttcgatgct ggtgacatcc aacagatcac cgatcagccg tgtcatccga gtcgtaaagc
     1801 gctggatcgc gtccgcgcgt tggacgactc gttgcccggc gtcgtctgga ggcgcttcat
     1861 gtatcagcat cgctgcgctg aggccgatgc cagcgatgag cgtccgcagg tcgtgactca
     1921 ccatgccgag aaaatcatcg cgcgctgtga tcgcgctgtc cgcgctgcgc cgctcgctca
     1981 gcaaactcgc atccgtgctc tgacgttccg tctcgagcaa ggcggcgatg acgcgatgac
     2041 ggtcagcacg ctcgatgttg cgttgacggt cgacgacctc acgttcgccg gcgagaagat
     2101 catcatcctg acgacgctcc gccgccaggc gaccgatttc gtccggctcc ggtgatcgca
     2161 ggtcggctgc ctcgcgagta gcccggacga cttcgtcggc tttcgcccgc gcgttcgcga
     2221 cgactgcatc ggcatcgtcc tcgatgtccg cgcgacgaag cgcgagctcg tcgtcggtct
     2281 tgcgacgttc ctcgccgagg ctttcgtccg tagcatcgcg atcctttgcc ttctgattgt
     2341 ccggcaccgt cgatccatac cacgagcgtc gtgctcggac tcgtggcacc tccgcgtcgt
     2401 cttccggctg tgggcgttcg tgagcaccaa cctgcccaac gagatcgagc tcggccctcc
     2461 caccggcgcc gagctcgcgg cgatcgacgc catgatccgt gtgtgcgcgc tgccggtcga
     2521 ggtggtgcgc gatcggttcc cagaggcgta tgtctccacg gccgcgccag cgccgccgag
     2581 ctctcgccgc ccaccgcgac cagcccgagc tcctgcagcg ccgagcgcga gccactccgg
     2641 gagtccctgc tcacgatcgc cgccgaccgc actggcccca ccgccgcctc gaactcgccg
     2701 cggaccacct cgactaagcc gacgtcgacg aggccccgcg cgatcgccgc cgccaccgcg
     2761 tggctcgctg ctcgcggcct agccgcgggt tgttcactta ggcacgacca cggcggcttg
     2821 ggctatgcaa tccatgccag actgggtgcg gtcccctccg acggcggggg cacttccgcc
     2881 cttggcgcgc acgaggtcga tgttgtcgag cgtccgtacg gtttcgctac gtaccaccag
     2941 cttgcgggta attttcttca ttgcgtcacg atagcgcacc gctcgtcgtg atcgcacgcc
     3001 tcaaggactt gctcgggctt gggtaaggac catcgtgcaa tatcgcccaa aaatccacgc
     3061 gttacgatgt cggcaccctc gacatggacc ccgataagat gggagcgatg cgcgggcatc
     3121 gtccacagcg gacgacccga agcggcctcc cagaaccgca actggccatc gccgccacca
     3181 ccgatgagca tggtgccgtc cgcggagagc ttggcatcaa caaggaagcg cgagccgcca
     3241 cggtacgtcc gatgcagttc gcccgtctcc gccccccaga gccgtacagc tccatcgccg
     3301 catgcggtga tcacgtcatc ccttgcgacg aagcgcgcag agtaaacctg cccttgacca
     3361 cttgcagcaa gctgagcaac gggacggtag agctccatat cccacagttc tggtgcaacg
     3421 accttctctg tgaatctcga aactgtgatt aggcgacggc tgtctgggga cattcgtagc
     3481 attgccacac gcgctagcgt atggagctcg gcaagagtcg tcccacctgg gtcgtagacg
     3541 cgcaaacgcc ctccagcatc tgccacgatc actcggccat cggccaggaa tccggctgca
     3601 tcgataccag acgacgacct cggcaacgct atcatcgcgc cgttgtctcg cgtgacgatg
     3661 atggatccat caatggcgcc gctgacgatg tcgcgcccag tagcagcgaa tgcgaccgcg
     3721 ttgacgggtg cgccgtgccg gatcgtgctc aacagccgag ccgccggtag ttcgtagacc
     3781 tccacgttgt tgccacgcgc aatcgccgcg cggtctccag caagagagac ggccggatat
     3841 gccgacgtga aatccccctc gaccagggtc acgcttggca gttcggcgac gagatgatcg
     3901 tgcgccgtgt cccaaatgcg tgtcggttgg tcgttgcagg tgatggccaa aaatcggcgg
     3961 tcgggctcaa ggctggtgac gagaccgcag ttgtcggtga acggcgagct ccaacggcga
     4021 tacggtgccg tcgcgtccca gatccggacg gtcccatccc aggaagcacc gacgacacgc
     4081 cgcgaagtcg ggtcgaagtg agcaacccgc actttggtgg caggtccgtc aaggcccgtg
     4141 accggcatcc ccgatgcggc ctcgcttacc gcaacagcgc cgtttgagcc agacgccgca
     4201 atcaacgacg aggcccgatc gaattcaacg gagaggactt cgcttcgaag gtagttgctc
     4261 tggctccgca acttcgcgga cgtcgcgtcc cagacttgca ccgcaccgtc gcggctaccc
     4321 gcggcgatca gtcgaccgtc aggtgagaac gctaccgtat cgatcggctc tccgagttcg
     4381 cggaggcgct gaactcgtcc gccgctgggg agtttccaga tcgacgcgtc accctccgcg
     4441 ctgccggtca cgagacggga acctgttgga tcccagctca ggctgtggat gcttagtccc
     4501 gagatgttgt gcgctaccgt ccaggtctgc gtgtcgagca catccacgtc gttgcccgtg
     4561 cccatcgcga gccagcggcc atcggagctg aatgcgagcg atggataggt tgatggcgcg
     4621 gtgactcgca ggtccgcgac cagcatgccg gtgccgatgt tccagacgtg ggccattctg
     4681 taatcgaggc cgacgacgag ttttccgtcg ggagacagag ctacgacgcg gtaacgaggt
     4741 ttgaccgtgt cgtgccggag ctctcgcagg agcactccgg ttgcagggtc ccagctcctg
     4801 acggcgccat cgccgcagcc ggtgaccagc ttggtgccgt cggcgctgta caccgcgtga
     4861 tagacgatgt cgccgtggtc caacgtgaac atccgccggt aggtctgggc gatccagacc
     4921 tgcgcggcac gatcgtcggt cgtgacaatc tggcggccat ccggggagaa ggcggctgac
     4981 cacatgcgcc cggatgtgct cgtgagacgc gcttgctcgg ctagcctggg ctgcagcgcg
     5041 cgagcgagca tgaacgccgt gatcggcgaa tggtcgccgc gctggtaggc gcggccgagg
     5101 tggatcgacg cctcgagctc gccgtggagc agcgccgatc gccctgctcc agctcggact
     5161 cggtgatggt cgcctccgtc acccgtcggt cagcgcgggc ctgcgtcacc gtgcggtaca
     5221 ggaacccgcc aagcacggtc accgcggcga gcgcgaccag tgcccgcttg gccaaacggc
     5281 tgcgccggat gcgccgctgg ctcgcggcga caaacgagcg ctccagcggg ccgattgtct
     5341 gctggggcac gctccgcagg gtttcctcca gcacgt
//
LOCUS       DASVOR010001769         2260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_193818,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001769 DASVOR010000000
VERSION     DASVOR010001769.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193818"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="VF516_32925"
     CDS             complement(<1..1206)
                     /locus_tag="VF516_32925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8112596.1"
                     /translation="MTSAARILVVDDDRSIRRTLEKFLGGEGYAVATAQDAPGAIAAA
                     DAADLMLLDLGLPGGSGFDVLTALAERPRPPTVVVITARDDMQSTVKAIQLGAYEYLV
                     KPIDIDRLRGVIRQALDSRETRDQLEAFVTRSAEARMGGDILGKSPAIRDVWKQIGAV
                     STTRAPVLIIGESGTGKELVARAIHVSSADRDRPFVAVNCTALAPGVLESELFGHVKG
                     AFTGAIADRPGRFELAGKGTLFLDEIAEIPLDLQAKLLRVVQERTFERVGDARPIALE
                     ARIIAATHRDLAAMVARGSFREDLFYRLRVVEIALPALRERAGDIPILVEGLLAKINR
                     DLGKAVRYVTPAALARLSAHGWPGNVRELENLMQYVAAAHQDEVLTADHVTERLAVRT
                     RAKSEPTEPA"
     gene            1877..>2260
                     /locus_tag="VF516_32930"
     CDS             1877..>2260
                     /locus_tag="VF516_32930"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8112597.1"
                     /translation="MDALWTLPELVAEVAARIAALPAPKNGQVRAVPDERTVRYYVTL
                     GLLDRPSAMRGRTALYGPRHVAQVVAIKRLQTMGRSLSEIQALWSTLDDPTLARMSGV
                     ALPAAARPAARAEFWKREPGQGDAAA"
BASE COUNT          329 a          799 c          803 g          329 t
ORIGIN      
        1 cgccggttcg gtgggctcgc tcttcgcgcg cgtgcggacg gcgagtcgct cggtcacgtg
       61 atcggcggtc agcacctcgt cctgatgcgc cgccgcgacg tactgcatga ggttctcgag
      121 ctcgcgcacg ttgcccggcc agccgtgggc cgacagccgg gccagcgcgg ccggcgtgac
      181 gtagcggacc gccttgccga ggtcgcggtt gatcttggcg agcagtccct cgacgaggat
      241 cgggatgtcg cccgcgcgct cgcgcagcgc cggcagcgcg atctcgacca cgcgcagccg
      301 gtagaacagg tcctcgcgga agctgccgcg cgcgaccatc gccgccaggt cgcggtgggt
      361 cgcggcgatg atccgggcct cgagcgcgat cggccgcgcg tcgccgaccc gctcgaacgt
      421 gcgctcctgc accacgcgca gcagcttggc ctgcaggtcg agcgggatct cggcgatctc
      481 gtcgaggaac agcgtgccct tgccggcgag ctcgaaccgt ccgggccggt cggcgatcgc
      541 gccggtgaac gcgcccttga cgtggccgaa cagctcgctc tccagcacgc cgggcgcgag
      601 cgccgtgcag ttgaccgcga cgaacggccg atcgcggtcg gccgacgaga catggatcgc
      661 gcgcgcgacc agctccttgc cggtgccgga ctcgccgatg atcagcaccg gggcgcgcgt
      721 ggtcgacacc gcgccgatct gcttccacac gtcgcggatc gccgggctct tgcccaggat
      781 gtcgccgccc atccgcgcct cggccgaccg ggtcacgaac gcctcgagct gatcgcgggt
      841 ctcgcggcta tcgagcgcct gccggatcac cccgcgcagc cggtcgatgt cgatcggctt
      901 gaccaggtac tcgtacgcgc cgagctggat cgccttgacc gtcgactgca tgtcgtcgcg
      961 cgcggtgatc accacgacgg tcggcggccg cggccgctcg gccagcgcgg tcagcacgtc
     1021 gaacccgctg ccgccgggca ggccgaggtc gagcagcatc aggtcggccg cgtcggcggc
     1081 cgcgatcgcc cccggcgcgt cctgcgcggt cgccaccgcg tagccctcgc cgccgaggaa
     1141 cttctccagc gtgcggcgga tcgagcgatc gtcgtcgacg accaagatgc gcgcggcgga
     1201 ggtcacgggt cagccctgtt cacggaccat gccatcgtaa cccatcgagt tcacgcgcgg
     1261 gtcccggcgc cgcgggtaac gaaacgtgac gcgcggaagc cttctcgtgg cgggccgcgt
     1321 cctcacgcgt ccgccgccgg ctcgccgagc cacgcgcgcg cgagcgcgaa ggtccgggcg
     1381 ttcaccgcgc gaagagcagc atcggagcca cggacgtgca ggaagctggc gccacggcat
     1441 ggcgtcggag atcagctctg ccgagaccgc gacatgtcac gtatgcgaac ctccacctcg
     1501 cgaccacgac atgcgccgac gtccgctcgt gcctcctcat tccgccgccg cgcgacggga
     1561 cctgcttgcg cagtacacgg cgcggtactg gcgcgaacgc aagatcgcga agccgatctc
     1621 cagatgcaca tcctgatgtc cgcggcgctt gcgcgcacgg cgccggggca gatccaggca
     1681 caccccgagc cggccggtag agcagcgcat cgcgcaggat ccatcggtcg ccaacgtcgt
     1741 cttcgcaaag cgcgctgatc tggaacgctg cgggcgcgtg ttcggggagc ctcgagtttc
     1801 tgatccaaga tattgatttc ttatgtcttt caagaaccta tttgacagtg aatataacag
     1861 tgttaaatag gacggcatgg atgccctgtg gaccttgccc gagctggtcg ccgaggtcgc
     1921 ggcgcggatc gccgcgctgc cggcgccgaa gaacgggcag gtccgcgcgg tacccgacga
     1981 gcggacggtt cgctactacg tgacgctggg gctgctggat cggccgagcg cgatgcgcgg
     2041 ccggacggcg ctgtacggac ccaggcacgt ggcgcaggtg gtggcgatca agcggctgca
     2101 gaccatgggc cgctcgctgt cggagatcca ggcgctgtgg tcgacgctcg atgatccaac
     2161 gctggcccgg atgtcgggcg tggcgctgcc ggcggcggcc aggcctgccg cgcgcgccga
     2221 gttctggaag cgcgagccgg ggcagggcga cgccgccgcc
//
LOCUS       DASVOR010001770         2436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_456402,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001770 DASVOR010000000
VERSION     DASVOR010001770.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456402"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="VF516_32935"
     CDS             complement(<1..803)
                     /locus_tag="VF516_32935"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8112598.1"
                     /translation="MIGGTYQTRPALPFVPGLEAAGRIASAPADSGFTTGDRVVAFLW
                     HGGYAERAVAAAAETFRLPQGMSFETAAALTSVHTSADLALRRSGQLTPSETLLVMGA
                     SGGVGLAAVQIGKAIGAHVIALVSRPDRLEFARQAGADHVFCHQHNEAWKHQVLDAVG
                     PGGVNVCIDPVGGSLFDSALSTLGWGGRYVLVGFAAGQVQQIPANRLLVKHRAALGCS
                     LRYFRRFAPSALRQSVEQLFAWYQQRKVAPFISARLPLSQTAQGLRALA"
     gene            complement(941..1915)
                     /locus_tag="VF516_32940"
     CDS             complement(941..1915)
                     /locus_tag="VF516_32940"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8112599.1"
                     /translation="MMYRTLAGIEVSVLAVGGASFGGIGSARRLIGRGESEADAHVLL
                     DCAVALGINLVDTAGTYGDGASERIIGAWLRARGAAVRDKVRIASKVGIRGGLGRRHV
                     FEEVDRSLGRLGIDTLDFYLAHVPDPARPWDEVLRTFEMLVERGKVRQIGVSNASAAD
                     LSALAAARGGGFRFVQNQFNLLHREDADNGVLASCQKLGLQYTPYSPLAGGLLSGAYR
                     LEGAIPEGTRVGLRRDIYASAWTPDNAARVVRLKAEAAAHSISPAGLATWWLLHCPFV
                     TSIMMGARRPDQLEALVGEALLLPPDEALWRGLGELPGVEAPCRGARP"
     gene            complement(1915..>2436)
                     /locus_tag="VF516_32945"
     CDS             complement(1915..>2436)
                     /locus_tag="VF516_32945"
                     /inference="COORDINATES: protein motif:HMM:NF021049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiD C-terminal domain-containing protein"
                     /protein_id="HEX8112600.1"
                     /translation="AGEVLPRSEHREILHHLTASLHAQIPMTAFLRIAFVELERDRIV
                     LAAPLLPSRNHRDTAFGPGVFTAAALAPWILLVRQAWTERLAVQILLRQCELAIHRPI
                     TTDYRAGCGAVPALDTEALRRGEKVRVSASSQVVFDDGAPAATYRAHYTLVPAAPGAN
                     PGDLQLPFPEEWR"
BASE COUNT          381 a          859 c          864 g          332 t
ORIGIN      
        1 ttcgcgagcg cgcggagccc ctgggcggtc tggctcagcg ggagtcgcgc gctgatgaac
       61 ggcgccacct tccgctgctg gtaccacgcg aacagctgct ccacggactg ccgcagcgcg
      121 gacggggcga accggcgaaa gtatcgcagc gagcacccca gggcggcccg gtgcttgacg
      181 aggagtcggt tggccggaat ctgctgtacc tggcccgcgg caaagcccac gagcacgtag
      241 cggccgcccc acccgagcgt cgatagcgcg ctgtcgaaca gcgagcctcc caccgggtcg
      301 atgcagacat tcacaccgcc gggacctacc gcgtcgagca cctggtgttt ccatgcctcg
      361 ttgtgctggt ggcaaaatac gtgatctgcc cccgcctgac gggcgaattc caggcgatcg
      421 ggacggctca ccagcgcgat cacgtgcgcc ccgatcgcct tgccgatctg tacggcggcg
      481 agccccacgc cgccgctcgc gcccatgacc agcagcgtct cggacggcgt gagctgcccg
      541 gagcgacgca gcgcgagatc cgcggaggta tgaacgctgg ttagcgcggc ggcggtctcg
      601 aacgacatgc cctgcggaag gcggaacgtc tcggcggcgg cggcgaccgc gcgctcggcg
      661 tagccgccgt gccacaagaa ggcgacgacc ctgtctccgg tcgtgaaccc cgagtccgca
      721 ggcgcgctcg cgatccgccc cgcggcctcc agcccgggga cgaagggaag cgccggcctg
      781 gtctggtacg tgccgccgat catcaagagg tccgcaaagt tcgcgctgca ggcgtggaca
      841 tcgatcgcca cgtctccggc gccgggaacc ggttccgcga tgtcctggag cgatagctgt
      901 tccggctcgc cccacgctgg gcagaccatc gcgcgcatgg tcacggcctt gctcctcgac
      961 aaggcgcttc cacccccggt agttcgccaa ggccacgcca cagcgcctcg tctggcggga
     1021 ggaggagagc ttcaccgacc agggcctcca gctgatctgg ccgacgtgcg cccatcatga
     1081 tcgacgtcac gaaggggcaa tgcagcagcc accacgtggc cagccccgcg ggggagatgg
     1141 agtgggcggc ggcctccgcc ttcaaacgta cgacgcgggc ggcgttgtcc ggagtccacg
     1201 cgctggcata gatgtcccgg cgtagcccga cgcgggttcc ctccgggatg gcgccctcca
     1261 gccggtacgc cccgctcagc aggccgcctg ccagcggtga gtagggcgtg tactggagcc
     1321 cgagcttctg gcatgaggcg agcacgccat tgtcggcatc ctcgcggtgg agcaggttga
     1381 actggttctg cacgaagcgg aagccgccgc cgcgcgccgc ggccagcgcc gagaggtctg
     1441 ccgccgaggc gttcgagaca ccgatctgcc gcacctttcc gcgttcgacg agcatctcga
     1501 acgtgcgaag gacctcgtcc cagggacggg cgggatccgg cacgtgcgcg aggtagaagt
     1561 cgagcgtgtc gatgccgagc cggccgagcg agcgatccac ctcctcgaag acgtgccggc
     1621 gtccgagccc gccgcggatc cccaccttcg aggcgatgcg gaccttgtcg cgcacggccg
     1681 caccccgggc gcggagccac gcgccgatga tgcgctcgct cgcaccgtct ccgtaggtgc
     1741 ccgccgtatc cacgaggttg atgccgagcg cgaccgcgca atcgaggagg acgtgcgcgt
     1801 cggcctcgga ctccccccgg ccgatgagcc ggcgagcgct gccgatgccg ccaaagctgg
     1861 ccccgcccac cgcaagtacc gagacctcga tcccggcgag cgtccggtac atcatcagcg
     1921 ccactcctcg gggaacggga gctggagatc gcccgggttc gcgcccggag cggcgggcac
     1981 gagcgtgtag tgcgcgcggt aggtggccgc cggtgctccg tcgtcgaaca cgacctgtga
     2041 gctggcggac acccggacct tctctccgcg ccgcagcgcc tcggtgtcga gcgctgggac
     2101 ggcgccacag cccgcgcggt agtcggtcgt gatggggcgg tggatggcga gctcacactg
     2161 ccggagcagg atctgcacgg ccaggcgctc ggtccacgcc tggcgcacca gcaggatcca
     2221 cggggcgagg gccgcggccg tgaacacgcc gggcccgaac gcggtgtcgc ggtggttgcg
     2281 cgacggcaag agcggcgccg ccaggacgat ccgatcgcgc tcgagctcga cgaaggcgat
     2341 gcgcagaaat gccgtcatgg ggatctgggc atggaggctg gcggtcaggt ggtgcaggat
     2401 ctcccgatgc tcgctccggg gcagcacctc accggc
//
LOCUS       DASVOR010001771         4337 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_397476,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001771 DASVOR010000000
VERSION     DASVOR010001771.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397476"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            486..1193
                     /locus_tag="VF516_32950"
     CDS             486..1193
                     /locus_tag="VF516_32950"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8112601.1"
                     /translation="MASLAGCAENQVTHTAPETPTTGTDRATVTGHVLDTTGAPVAGA
                     TVTVRSSGEQATVDSTGAFTLDVPANTTLTLSATAPSMAPTLLQQFLVSTGTTAQVEI
                     PLITGDHFKSLVAMGMNPSGGVVAVAVKSLSGAGSVGGATVQLTPSLGRMMYAPAGAG
                     MADPDPAMAAVVPSVAQGDESYAWALGVQPHVSIMQFALHGVAQIEPPYAIDDVIWPG
                     SFTVDAGALTLVTLFTK"
     gene            complement(1209..2807)
                     /locus_tag="VF516_32955"
     CDS             complement(1209..2807)
                     /locus_tag="VF516_32955"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8112602.1"
                     /translation="MSSHSRSAAAETFDSCPADKPSRDAGRLCYEPCKPGFEGVGPVC
                     HRVTALRDGTCNSDEDKDGALCYPKCKPGYTGVGPVCWQNCPGGFRDDGAFCGKPDSY
                     GRGAGYPWKLGDPIDSLKPAKKRCEHDHDQGCEKDGAIYYPKCKHGFKSAGCCVCSPK
                     CEDGMTDIGVSCAKQSYGRGAGRDPESYGRGVGVSLTSCTSGLVWKPIGQDRTAPPFF
                     LVIASDPQLDWYSKDDCSGPACELPFGDCKDEACGLKNAFKTNDQQLAAINAIMTLAW
                     PYGAVTPPKGVIFNGDLTAYGHPQQWDWFMWYYVNRLRWPAYYGLGNHDYENNVGSCY
                     GATTDKDWCARNSIEWMRSTVVCGRSKPFPANTITGYDDASMAYSWDIGPYHFVQLQN
                     HPLYPKDEKTGKTKPVPGIESSFTWLTSDLKRATAAGQHIIINMHDFGDHMHTDNAQF
                     AAAIAGSNVRAIFRGHIHHLVGGSAHIVVDGRNVPVFYSGSSVYRKFLLVEFLASGLR
                     VAEIDAAQGTPKLLWPNAVHGVAF"
     gene            2818..3054
                     /locus_tag="VF516_32960"
     CDS             2818..3054
                     /locus_tag="VF516_32960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112603.1"
                     /translation="MIATAAMLVLLQAIGIMFDPPPSRFTVHDRLLHGSDSARQCVDP
                     IAPRIGRAASAARVRVIGSERPHGRVRARYVDEL"
     gene            3259..>4337
                     /locus_tag="VF516_32965"
     CDS             3259..>4337
                     /locus_tag="VF516_32965"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8112604.1"
                     /translation="MLRLVSMSLLAVAALAAPAAADPVHGLVYDDRNHDGQPNAGEPG
                     VAGAVVAFGVQQFVVTDAQGQFTLDLPPGAQGIAWVRVPDGFAPGPVWAHVGGDRPGP
                     EIDLGLRRLAAPHRGPITFVVAADTHLAQDQPFFDDLGQVAADATALDPPPAFFTILG
                     DITQGNQLQHFMLVDRALAGLNAPYVPVPGNHDWYDGGATWFSHYGPDNYSFDIDTTH
                     FVVWNMAMSEQQIRDYLGAELSHVAQGMSIVALTHAPPSEGVTDALRSLGVNYVLTGH
                     THSNRVVDHDGVIELNSEPLLMGGLDFTPGGYRVMTIDRGALTTYHRTVVERPLLEVM
                     APAAGQCVPLTGGQVVAAAESDAGDP"
BASE COUNT          679 a         1551 c         1418 g          689 t
ORIGIN      
        1 acaccgcgac cgacgccgag tacaagatcg gcaagcagga cggcaagcgc acgatctaca
       61 acctgcgcta caaggactac atgtggctcg acgacaagtc gccctacgtc agcagcaagg
      121 gcgatccgaa gaaggacagc gcgcagtggg tgatcgacgg cctgccggat agctggtagg
      181 tcgcgatccg cgaatggcgg tggggcaacg ggtccgggga gcgcccccct ggacctccgg
      241 aatggccccc tggactttcg ggatggcccc tggactttcg ggacggcccc ctggacctcc
      301 gggatggccc cccttggacc ttcggtgccc gggcgcgatg aatccggttg gcggcggaag
      361 ccctcgatac gaatggcgat ctgatcggtg gtcgccgcga gcaggtcgac gccatgactt
      421 cggcattgcc cgtgcagtag ggcctgtccg tcatgatgaa ccgagtcgct ccgttcgttg
      481 tcgtggtggc ctcgctcgcg ggttgtgcag agaaccaggt aacccacacc gcccccgaga
      541 cgcccaccac cggcaccgac cgcgcgaccg tcaccggcca cgtcctggac accaccggcg
      601 cccccgtggc gggcgcgacg gtgaccgtcc gtagctccgg cgagcaggcg accgtggaca
      661 gcaccggcgc gttcaccctc gacgtcccgg ccaacaccac gctgaccctc tcggcgaccg
      721 cgcccagcat ggcgcccacc ctcctccagc agttcctggt ctcgaccggc accaccgccc
      781 aggtcgagat cccgctgatc accggcgacc acttcaagag cctggtcgcg atgggcatga
      841 acccctcggg cggcgtggtc gccgtcgcgg tgaagtcgct gtcgggcgcc ggcagcgtcg
      901 gcggtgccac cgtccagctc accccgagcc tgggccgcat gatgtacgcg ccggccggcg
      961 ccgggatggc cgacccggac cccgcgatgg cggcggtcgt gccgtcggtc gcgcagggcg
     1021 acgagtcgta cgcctgggcg ctcggcgttc agccccacgt ctcgatcatg cagttcgcgc
     1081 tgcacggtgt ggcgcagatc gagccgccgt acgccatcga cgacgtcatc tggcccggca
     1141 gcttcaccgt cgacgccggc gcgctcaccc tggtcacgct gttcaccaag tgagcgccgt
     1201 tgccgcgctc agaacgcgac gccgtgcacg gcgttgggcc agagtagctt gggcgtaccc
     1261 tgcgccgcgt cgatctccgc gacccgcagg ccgctggcga ggaactcgac cagcaggaac
     1321 ttgcgataca ccgacgagcc cgagtagaac acgggtacgt tgcgaccgtc gacgacgatg
     1381 tgggccgagc caccgaccag gtggtgaatg tggccgcgga agatcgcgcg cacgttggag
     1441 cccgcgatcg cggccgcgaa ctgcgcgttg tccgtgtgca tgtggtcgcc gaagtcgtgc
     1501 atgttgatga tgatgtgctg tccggcggcg gtcgctcgct tcaggtcgct ggtgagccag
     1561 gtgaacgacg actcgatacc gggcaccggc ttggtcttgc cggtcttctc gtccttcgga
     1621 tacagcgggt gattctgtaa ctgcacgaag tgataggggc cgatgtccca cgaatacgcc
     1681 atgctggcgt cgtcatagcc tgtgatggtg ttcgccggaa acggcttcga gcggccgcac
     1741 acgaccgtcg agcgcatcca ctcgatcgag tttcgagcgc accaatcctt gtccgtagtg
     1801 gctccgtagc acgagccgac gttgttttcg taatcgtgat taccgagacc gtaatacgct
     1861 ggccagcgca ggcggttcac gtagtaccac atgaaccagt cccactgctg cggatggcca
     1921 tacgccgtca agtcaccatt gaagatcacg cccttggggg gcgtgacagc gccgtacggc
     1981 cacgccaggg tcatgatcgc gttgatcgcc gcgagctgct gatcgttggt cttgaacgcg
     2041 ttcttgagtc cgcacgcctc gtccttgcaa tcgccgaacg gcagctcgca cgccggtccg
     2101 gagcagtcgt ccttcgaata ccagtcgagc tgcgggtcgg acgcgatcac caggaagaac
     2161 ggcggcgcgg tacggtcctg gccgatcggc ttccagacca ggcccgaggt acacgacgtg
     2221 agcgacaccc cgacgccgcg gccgtatgac tcgggatctc ggcccgcgcc gcggccgtac
     2281 gactgcttgg cgcacgatac accgatgtcg gtcatgccat cctcgcactt cgggctgcac
     2341 acgcagcagc ccgctgattt gaagccgtgc ttgcacttcg ggtagtagat cgcgccgtcc
     2401 ttctcgcagc cttggtcgtg gtcgtgctcg cagcgcttct tcgcgggctt gagcgagtcg
     2461 atcgggtcgc cgagcttcca cggataaccg gcgccgcggc catacgaatc cggcttgccg
     2521 cagaacgcgc cgtcgtcgcg aaatccgccg ggacagttct gccagcacac cgggccgacc
     2581 ccggtatacc cgggcttgca cttcggataa cacaacgcgc catccttgtc ctcgtcgcta
     2641 ttgcatgtgc cgtcgcgcag cgccgtcacg cgatggcata ccggacccac gccctcaaat
     2701 cccggtttac atggctcgta gcacagccga cccgcatcgc gcgatggctt gtcggctggg
     2761 cacgaatcga atgtctctgc cgccgcggaa cggctgtgcg aactcacgac cagcagggtg
     2821 atcgcgaccg cggcgatgct cgtcctgctc caagcgatcg gtatcatgtt cgatcctcca
     2881 ccttctcgct tcaccgtgca cgatcgcctg ctgcacggca gtgacagcgc gcgccaatgc
     2941 gtggatccga tcgcgccacg catcggtcgc gcggcgtcag ccgccagagt tcgcgtcatc
     3001 ggcagcgaac ggccgcatgg ccgggtacgg gcacggtacg tcgacgagct ctgaccgcgg
     3061 caccgcagca aggatccagc ggctcggcca cccaccgggt cacgaagtcc agccggtcgc
     3121 tgcgtaccag gcctgcgagc gcgcccacgg ccggacaccg cgaccgtagc tccacgctca
     3181 ccctgggcgc ctggccggcg atcacgtccg gtgcgatcgg cgggagccgc ccggtcgcat
     3241 cgcccgtata atgtaggcgt gctccggctg gtctccatgt cgctcctcgc ggtcgccgcg
     3301 ctcgcggcgc cggccgccgc cgacccggtg cacggcctgg tctacgacga tcggaaccac
     3361 gacggccagc cgaacgccgg cgagcccggg gtcgcgggag cggtggtcgc gttcggcgtc
     3421 cagcagttcg tggtcaccga cgcccagggc cagttcacgc tcgacctgcc gcccggcgcg
     3481 cagggcatcg cctgggtccg ggtgcccgac ggcttcgcgc ccggcccggt gtgggcccac
     3541 gtcggcggcg accgaccggg cccggagatc gatctcggcc tgcgccggct ggccgcgccc
     3601 caccgcgggc cgatcacgtt cgtggtcgcc gccgatacgc acctcgccca ggaccagccg
     3661 ttcttcgacg acctcggcca ggtcgcggcg gacgcgaccg cgctcgatcc gccgcccgcg
     3721 ttcttcacga tcctcggcga catcacgcag ggtaaccagc tccagcactt catgctggtc
     3781 gaccgcgcgc tcgccgggct gaacgcgccc tacgtcccgg tgcccggcaa ccacgactgg
     3841 tacgacggcg gcgcgacctg gttctcgcac tacggccccg acaactacag cttcgacatc
     3901 gacaccaccc acttcgtggt gtggaacatg gcgatgtccg agcagcagat ccgcgactac
     3961 ctcggcgccg agctgtcgca cgtcgcgcag ggcatgtcga tcgtcgcgct gacccacgcg
     4021 ccgcccagcg agggcgtgac cgacgcgctg cgctcgctcg gcgtgaacta cgtgctgacc
     4081 gggcataccc actccaaccg cgtggtcgac cacgacggcg tgatcgagct caactccgag
     4141 ccgctcttga tgggcgggct cgatttcacg cccggcggct accgggtgat gacgatcgac
     4201 cgcggcgcgc tcacgaccta ccaccgcacg gtggtcgagc gcccgctgct cgaggtcatg
     4261 gcgccggcgg cgggccagtg cgtgccgctg accggcggcc aggtcgtcgc ggccgccgag
     4321 tccgacgccg gcgatcc
//
LOCUS       DASVOR010001772         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_76678,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001772 DASVOR010000000
VERSION     DASVOR010001772.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76678"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..911)
                     /locus_tag="VF516_32970"
     CDS             complement(<1..911)
                     /locus_tag="VF516_32970"
                     /inference="COORDINATES: protein motif:HMM:NF039722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HEX8112605.1"
                     /translation="MPRPRNRVITGAISTAAPPRVARRHQVWWVALAVGIATFFAWIG
                     YARTLDPQNLTWIFHEDPFAHVMGWEQLRNAPIAQYPLTRNELYGLEWSSTLVFADSI
                     PAAALVLRPLSALLPRPFQYLGWWTLLTMVLQAYWGARLMLLRSQRLTDAAIAAVLFV
                     TTPVLLERVGLHTALGSHWLLLCALWLYLDARAVRPRAWAALLLLAVSVHAYLFVMIG
                     GIWAAHLVRCRLDGQLSRRDVVAAAITAAAVVAWMHALGYFVVGEGIAGGGWRSSVDL
                     LGFVAPARGALLGLAGVLTSDAWDGSA"
     gene            1236..1817
                     /locus_tag="VF516_32975"
     CDS             1236..1817
                     /locus_tag="VF516_32975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112606.1"
                     /translation="MFKITALLTTLALGSAASIAMASPARSYDSDGRFDPAEPAPPAW
                     TAPAAPPARSAWIDHARIDPTYDRAYDAGRFGRPYRRGALDDLGPRRYRASWVALSAP
                     APIRRAEDCIDVHDRGTFTQLRVQADGGRALVDRVVVQFADGSRQVEDLDRVLGGRSD
                     FVDIPLDGNNRRIDRIIVTGSTGRRGALQVFGI"
     gene            1887..2372
                     /locus_tag="VF516_32980"
     CDS             1887..2372
                     /locus_tag="VF516_32980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112607.1"
                     /translation="MYRAALLAIIATGGADTRPAGHCQLHVTLPTVKSPRRYDRTRSR
                     LVARGIVVLAALMLTACGRGKPDLCAGAVRHVLELTSGASKDGKPATDAITATTSRCQ
                     QEGLSQPQADCILAAQGPEWSDQLRACPAFAARPASWVALGPSQKASKPSGPAEQPRR
                     C"
     gene            complement(2484..>2683)
                     /locus_tag="VF516_32985"
     CDS             complement(2484..>2683)
                     /locus_tag="VF516_32985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112608.1"
                     /translation="PQKLLEKYDEFVKYLATIPDAPRRRDDGRMLAETAVRALADLKQ
                     CVGTAAQNKDALLFRLLPPPG"
BASE COUNT          388 a         1028 c          914 g          353 t
ORIGIN      
        1 taggcgctgc cgtcccacgc gtcgctggtc agcacgccgg ccagcccgag gagcgccccg
       61 cgcgcagggg ccacgaaccc gagcagatcg acgctcgatc gccagccgcc gccggcgatg
      121 ccctcgccca ccacgaagta gccgagcgca tgcatccacg cgacgaccgc ggctgcggtg
      181 atcgccgcgg cgacgacgtc gcgccggctc agctggccat cgagccggca gcgcaccagg
      241 tgcgccgccc agatcccgcc gatcatgacg aacaggtacg catgcacgct caccgcgagc
      301 agcagcagcg ccgcccacgc ccgcggccgc acggcgcgcg cgtcgaggta cagccacagc
      361 gcgcacagca gcagccagtg gctgccgagc gcggtgtgca gcccgacccg ctcgagcagc
      421 accggcgtgg tcacgaacag caccgcggcg atcgcggcat cggtgagccg ctgcgagcgc
      481 agcagcatca gccgcgcacc ccagtacgcc tgcagcacca tcgtgagcag cgtccaccag
      541 ccgaggtact ggaacggccg cggcagcagc gcgctcagcg ggcgcaacac cagcgccgcc
      601 gccgggatcg agtcggcgaa taccagcgtg ctgctccact cgagaccgta cagctcgttc
      661 cgcgtcagcg ggtactgcgc gatcggcgcg ttgcgcagct gctcccagcc catgacgtgg
      721 gcgaacgggt cctcgtggaa gatccacgtc aggttctgcg gatcgagcgt ccgcgcgtag
      781 ccgatccagg cgaagaacgt cgcgatcccg acggcgagcg cgacccacca gacctggtgg
      841 cgccgagcta cgcgcggtgg ggctgcggtc gaaatcgcgc cggtgataac gcgattccgc
      901 ggccggggca aaccgcgccg gcgccgacgg cgagccgcgg atcgccggag gtccggctcg
      961 cccccgcgcg gtccgttccg tgcctcaccg gcttcggccc gacaccccgc cggcagccat
     1021 gcgctcactg gctggagcgg cgtgtgctat ctgctcgcgc gatgatccgc gctggtggac
     1081 cgcgcgccgc cgtgctcgtg gatcttcgca gtcctgccgg acccgcagcc ggcgctgccg
     1141 gatctgcagt gccgcgcgcg cgggcgacac cgatctcgcg agcttgcgcc aggcccgctg
     1201 gctgcactgg gtccctcctc gggaggaacc cactcatgtt caagatcaca gcactgctca
     1261 cgacgctggc cctcggatcg gccgcgtcga tcgccatggc ctcgccggcc cgctcgtacg
     1321 atagcgacgg tcgattcgac cccgctgaac ccgccccgcc cgcgtggacc gcgccggccg
     1381 cacccccggc gcggtcggcc tggatcgatc acgcgcgcat cgatcccacg tacgatcgcg
     1441 cgtacgacgc cggccgcttc ggccgcccgt atcgccgcgg cgccctcgac gacctcggcc
     1501 cgcgccgcta ccgggcgagc tgggtcgcgc tgagcgcacc ggcgccgatc cgccgcgccg
     1561 aggactgcat cgacgtccac gatcgcggca cgttcaccca gctccgcgtg caggccgacg
     1621 gcggccgcgc gctggtcgat cgcgtcgtcg tgcagttcgc cgacggctcg cgccaggtcg
     1681 aggatctcga tcgcgtgctc ggcggtcgca gcgacttcgt cgacatcccg ctcgatggca
     1741 acaaccgccg gatcgaccgg atcatcgtca ccggttccac aggccggcgc ggcgcgctcc
     1801 aggtgttcgg catctagccg gctcgatcgc cggaagtccg gctcgcgccc gcgcggtccg
     1861 ttccgtgcct caccatcaat cccgccgtgt accgcgccgc cctgctcgcg atcatcgcga
     1921 ccggcggcgc ggacacgcgc cctgccggcc attgccagct gcatgttacg ctgccgacgg
     1981 tgaagtcccc tcgacggtac gatcgaaccc ggagccgcct cgtcgcacgg ggcatcgtcg
     2041 tgctcgccgc gctgatgctc accgcctgcg gccgaggcaa gccggacctg tgcgccggcg
     2101 cggtgcgcca cgtgctcgag ctcaccagcg gcgcctcgaa ggatggcaag ccggcgaccg
     2161 acgcgatcac cgccacgacg agcaggtgcc agcaggaggg cctgtcgcag ccccaggcgg
     2221 actgcatcct cgccgcgcaa gggcccgagt ggagcgatca gctgcgcgcg tgcccggcgt
     2281 tcgcggccag gccggcgagc tgggtcgcgc tcggcccgag ccagaaagcc agcaagccgt
     2341 cagggcctgc cgagcagccg cgccggtgtt gacctgctcg acgaataccc ctaattgcgt
     2401 cgcgcatctg gccactggca tctgccatct actcccggcg atggcacggt tctgtctcga
     2461 agtggtgatc cacagggcgc agctcacccc ggcggcggca gcagccggaa cagcagcgcg
     2521 tccttgttct gcgcggcggt cccgacgcac tgcttgaggt cggcgagcgc gcggacggcg
     2581 gtctcggcga gcatccggcc gtcgtcgcgc cggcgggggg cgtcggggat ggtcgccagg
     2641 tacttgacga actcgtcgta cttctcgagc agcttttgcg gat
//
LOCUS       DASVOR010001773         2411 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_106904,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001773 DASVOR010000000
VERSION     DASVOR010001773.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2411
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106904"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2411
                     /locus_tag="VF516_32990"
     CDS             <1..>2411
                     /locus_tag="VF516_32990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112609.1"
                     /translation="LFLAMDFVEGRDLRAVWNRCAKKQVAFPIDVAVYIVKELCRGLA
                     YAHAFPDLHLVHRDISPPNILVSYTGEVKLTDFGLASSTMKLEKTAPGIIYGKVAYMS
                     PEQARGEKLDGRSDMYAAAIVLWELLTGRQLFPPGKDQPQDLLTRAKNPEPPRPSKRA
                     PRVPVELDEICLKALAPSREDRYADCDAMRMALQTWLAQNAPTTDGTRMAAFLQELFT
                     EDIQRERAERNELLTRVRTRALTLPPTDELRQLFEGNKSEAATLPGDTGASHDAPVRR
                     PGSVGRRASDRQVEIIDRRQSPDRRNATTRPATDHSAGAAALAAKVEIHADAEPDLIG
                     TEVNDRYRVLELIGEGGMGKVYLAEHVEIGKRVALKVLHPSYSRMPDLVERFRREARA
                     ASKIGHPNIVDVTDSGTTADGSVYFVMEYLEGVELGSVIEREGALDVARALKITGQIC
                     RALAAAHREGIIHRDLKPENIFLITRDGAADVVKVLDFGIAKTTEAEAARERRLTSPG
                     MAMGTPEYMSPEQAAGRAADARCDVYALGAIMYEMVTGIPPYSGDNFMEILTKKATVD
                     PPPPLLVRGELPAQVSDLVMAAMARNPGDRPQSMETLEYELNKCLAGRGVAVAQILGM
                     TTDAHVVATLNPGLSMRTFDDAVVVARAQTSSPGGLPRPGTHSGMSELPGMWSGPVMA
                     TGQSALMARQATDPGVAGYLGPGQGPAASGSFPAQGIPLRAQSSPSLIQPAVTISPAV
                     SDPGLPAAPPMVVVHRSALGVLGWIVLAAVLFGGVGSLLYIAIGERNAVRASQAAARA
                     GGSGK"
BASE COUNT          365 a          866 c          873 g          307 t
ORIGIN      
        1 gctgttcctc gcgatggact tcgtggaggg ccgcgatctg cgggcggtat ggaaccgctg
       61 tgccaagaag caggtcgcgt tcccgatcga cgtcgccgtc tacatcgtga aggagctctg
      121 tcgcgggctg gcctacgcgc atgcgtttcc ggatctccac ctcgttcacc gcgacatctc
      181 gccgccgaac atcctggtgt cgtacaccgg cgaggtcaag ctgaccgact tcgggctcgc
      241 gtcgtcgacg atgaagctcg agaagaccgc gccgggcatc atctacggca aggtcgcgta
      301 catgtcgccc gagcaggcgc ggggcgagaa gctcgacggg cgcagcgata tgtacgccgc
      361 cgcgatcgtg ctgtgggagc tgctcaccgg gcgccagctg ttcccgccgg gcaaggacca
      421 gccgcaggat ctgctgacgc gggccaagaa ccccgagccg ccgcgcccga gcaagcgcgc
      481 accgcgcgtg ccggtcgagc tcgacgagat ctgcctcaag gcgctcgcgc cgtcgcgcga
      541 ggaccgctac gccgactgcg atgcgatgcg catggcgctg cagacctggc tggcgcagaa
      601 cgcgccgacc accgacggca cgcggatggc ggcgtttctc caggagctgt tcaccgagga
      661 catccagcgc gagcgcgccg agcgcaacga gctgctcacg cgggtccgga cccgcgcgct
      721 gaccctgccg ccgaccgacg agctgcgcca gctcttcgag ggcaacaagt ccgaggcggc
      781 gacgctgccg ggcgacaccg gggcgagcca cgacgcgccg gtgcgcaggc cgggctcggt
      841 cggccggcgc gccagcgatc gccaggtcga gatcatcgat cgccggcaat cgcctgaccg
      901 gcgcaacgct acgacccggc cggcgaccga ccacagcgcc ggcgcggccg cgctggcggc
      961 caaggtcgag atccacgccg acgccgagcc cgacctgatc ggcaccgagg tcaacgaccg
     1021 ctaccgggtg ctggagctga tcggcgaggg cgggatgggc aaggtctacc tcgccgagca
     1081 cgtcgagatc ggcaagcggg tcgcgctcaa ggtgctgcac cccagctact cgcggatgcc
     1141 cgacctggtc gagcggttcc gccgcgaggc gcgcgcggcg tcgaagatcg gccaccccaa
     1201 catcgtcgac gtcaccgact ccggcaccac cgccgacggc agcgtgtact tcgtcatgga
     1261 gtacctcgag ggcgtcgagc tcggcagcgt gatcgagcgc gagggggcgc tcgacgtcgc
     1321 gcgcgcgctc aagatcaccg gccagatctg ccgcgcgctg gccgcggcgc accgcgaagg
     1381 catcatccac cgcgatctca agcccgagaa catcttcctg atcacgcgcg acggcgccgc
     1441 cgacgtggtc aaggtgctcg acttcgggat cgccaagacc accgaggccg aggccgcgcg
     1501 cgagcgccgg ctgaccagcc ccgggatggc gatgggcacg cccgagtaca tgtcgcccga
     1561 gcaggccgcc ggccgggccg ccgacgcgcg gtgtgacgtc tacgcgctcg gcgcgatcat
     1621 gtacgagatg gtgaccggca tcccgccgta cagcggtgac aacttcatgg agatcctgac
     1681 caagaaggcg acggtcgatc cgccgccgcc gctcctggtc cgcggcgagc tcccggcgca
     1741 ggtgtccgac ctcgtgatgg ccgcgatggc gcgcaatccg ggcgaccggc cgcagagcat
     1801 ggagacgctc gagtacgagc tcaacaagtg cctcgccggg cgtggcgtcg cggtggcgca
     1861 gatcctcggg atgaccaccg acgcgcacgt ggtcgccacg ctcaacccgg ggctatcgat
     1921 gcgcacgttc gacgacgcgg tcgtggtcgc gcgcgcccag accagctcgc cgggcggcct
     1981 gccgcgaccc ggcacgcaca gcggcatgtc cgagctcccc gggatgtggt cggggccggt
     2041 gatggcgacc ggccagagcg ccctgatggc gcgccaggcc accgacccgg gggtcgcggg
     2101 gtatctgggg ccgggccagg ggccggccgc gagcggcagc ttcccggcgc agggcatccc
     2161 gctgcgcgcg cagagctcgc cgtcgctgat ccagccggcg gtgacgatct ccccggcggt
     2221 gtcggacccg gggctgccgg cggcgccgcc gatggtcgtg gtccaccgca gcgcgctcgg
     2281 cgtgctcggc tggatcgtgc tggccgcggt gctgttcggc ggggtcggct cgctgctgta
     2341 catcgcgatc ggcgagcgca acgcggtccg cgcgtcgcag gccgccgccc gcgcgggcgg
     2401 atccggcaag c
//
LOCUS       DASVOR010001774         1558 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_280387,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001774 DASVOR010000000
VERSION     DASVOR010001774.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280387"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            365..727
                     /locus_tag="VF516_32995"
     CDS             365..727
                     /locus_tag="VF516_32995"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112610.1"
                     /translation="MTTVLLVDDEPGVRFALSEVLHDRGYRVVSVGSGPEALAALDGV
                     DVVVTDLSMPGMDGLELVSQIAARAPRLPVILLTAHGSESLVRIASSRGACSCLSKPF
                     DIDEIARVVERALGRPRS"
     gene            863..>1558
                     /locus_tag="VF516_33000"
     CDS             863..>1558
                     /locus_tag="VF516_33000"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112611.1"
                     /translation="MTQLAELIGQLLIRGYNVDVMLPSLIGQYRIVRKIGAGGMGLVY
                     LGEHTLLHRRAAIKTLLPTVAANKQIVERFFTEARATSAISDPGVIQIFDFGYHVDGT
                     AYIVMELLEGEALSARLDRLGRLALGDALRFARQITGSLAAAHARDIVHRDLKPENIF
                     LVRDGEAQGGERTKIIDFGICKVGAGDPMLTEVGVMIGTPVYMSPEQCRGSGEVDARS
                     DVYAFGCLLFHMLT"
BASE COUNT          220 a          499 c          584 g          255 t
ORIGIN      
        1 ccctggaagc ccctcatcat gccgccgcgg cccggagtgt tggcggcgat gcgggctcgg
       61 gtagcgattc cgacaggtgc cggttgcttc tctttacagc gcctgcggcg gggttgcggg
      121 aaaccgcgta gcgggtcgtg ataccggcca cgccggggcc atgccggggc caggccgggg
      181 ccatgacaag acgggcgctt cggctcactt cgggcgccat cggggaggaa tccgggacaa
      241 agtgttcgtc gcgccggccg ccggacgtgg accgggcggc gcaccccctg cgcggccggt
      301 gcggcatggc tctggcaatc gcgaaggtcg tggacgatcc gttgcatcgc ggagccggct
      361 cgccgtgacc acggtcctgc tggtcgacga cgagcccggg gtccggttcg cgttgagcga
      421 ggtgctgcac gaccgcggct atcgcgtggt cagcgtcggg tcggggccgg aggcgctggc
      481 cgcgctcgac ggagtggacg tcgtggtgac cgatctatcg atgccgggga tggacggcct
      541 ggagctggtc tcgcagatcg cggcgcgcgc gcctcggctg ccggtgatct tgctgaccgc
      601 gcatggctcg gagagcctgg tccggatcgc gtcgagccgc ggcgcgtgca gctgcctgag
      661 caagccgttc gacatcgacg agatcgcgcg ggtggtcgag cgcgcgctcg gccgcccgcg
      721 gagctgaacg aacgttgcgg cgccgcacgg cgcgggagtt cggggcgacg cggcgcagcg
      781 cccggccaag tcgcgcatat tgcagcctac gagatcgtct gtgtgcagcg ctgtcacgtg
      841 cggattcgct cgacgtgccg tcttgactca actagcggaa cttattggac aattactgat
      901 tcgtggctac aacgtcgatg tgatgctccc cagcctgatc ggccaatacc ggatcgttcg
      961 caagatcggc gcaggcggga tgggcctggt gtacctgggc gagcacacgc tgctccaccg
     1021 gcgcgcggcg atcaagacgc tgctgccgac ggtggccgcc aacaagcaga tcgtggagcg
     1081 gttcttcacc gaggcccgcg cgacctcggc gatctcggat cccggcgtga tccagatctt
     1141 cgacttcggc taccacgtcg acggcaccgc gtacatcgtg atggagctcc tggagggcga
     1201 ggcgctgtcg gcgcggctcg accggctggg ccggctcgcg ctcggtgacg cgctgcggtt
     1261 cgcgcggcag atcaccggct cgctcgccgc ggcgcacgcc cgcgacatcg tccaccgcga
     1321 tctcaagccg gagaacatct tcctggtgcg cgacggggag gcgcagggcg gcgagcgcac
     1381 gaagatcatc gatttcggga tctgcaaggt gggggcgggc gatccgatgc tgaccgaggt
     1441 cggcgtgatg atcgggacgc cggtgtacat gtcgccggag cagtgccgcg gctcgggcga
     1501 ggtcgacgcg cggtccgatg tctacgcgtt cggctgcctg ctgttccaca tgctgacc
//
LOCUS       DASVOR010001775         4978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_486076,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001775 DASVOR010000000
VERSION     DASVOR010001775.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486076"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VF516_33005"
     CDS             <1..523
                     /locus_tag="VF516_33005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="benzoyl-CoA 2,3-epoxidase subunit BoxB"
                     /protein_id="HEX8112612.1"
                     /translation="FGGEVSSNAADAFAAGLKGRYKEDRYDEHKLVGSHFAMDVLEGG
                     KVVHKDIPMRNALNEVLRTEYIKDCQRGVDRWNKTIKDEGIDFELRLPSNKFHRQIGM
                     WAGQGFTPDGEMLDAAAFAARRGEWLPNDADEAYIKSLMHAVYEPGKMASWIAPPMRG
                     INGLPGEFVYVRM"
     gene            674..1081
                     /locus_tag="VF516_33010"
     CDS             674..1081
                     /locus_tag="VF516_33010"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEX8112613.1"
                     /translation="MLFRPNPSSGVPIYLQLMEQVKHAIETGALRPGEQLPGIRPLAE
                     ELVINPNTVAKAYRELEHEGVIELRHGAGAFVAAGPRAKKATDNLRAGQALVASTVER
                     LRARGLSDDEIRRLFEAELAGRAAAPRTGGQRG"
     gene            1074..1997
                     /locus_tag="VF516_33015"
     CDS             1074..1997
                     /locus_tag="VF516_33015"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8112614.1"
                     /translation="MAEPLVIETAGLCKRYGEPEGGRQGGIDALRGLDLQVPAGSICG
                     FLGRNGAGKTTTIKVLLGMAHASGGHARVFGLPVGDERASVEIRRRTGFVSDDKDLYD
                     YMDVAGMIRFTAAFYPRWRADLARDYLRRFELPPDRKVKSLSRGMRTKLALLVALCRG
                     AELLILDEPTAGLDPAMAEQVLQALVAHVAREATTVFFSSHQMAEVDQIADRIAIIDR
                     GRAVLSGALDDLRARFRRIQLVFDRDPPEIVLRAPVVGRVRRQGRVVSVLTSAGADPI
                     LDEVRALGPVSVDVAPVGLKEIFLETVAAED"
     gene            1999..2766
                     /locus_tag="VF516_33020"
     CDS             1999..2766
                     /locus_tag="VF516_33020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112615.1"
                     /translation="MLWHKSWLETRWRFLIGLGLLVCSAAGVVLAYPRVVELLALVPA
                     RDTGGELGRRIRESAELARDYRGYVWSQWFGQHLVQAWTLFAVLLGTGGLVSQAVRGG
                     ALFTLSLPVSRRRLVAVRAAVGLAELLVLAVVPSLLIAALSPAIGQRYGVADALVHGA
                     CLFVAGTVFFSLTSLLSTAFSDIWRPPLIVLLVAVVLGVSEQLVRDVARFSVFSVMSA
                     QRYFQGGGLPWLGLAASAAVSVALLYGAARNVARQDF"
     gene            2785..3501
                     /locus_tag="VF516_33025"
     CDS             2785..3501
                     /locus_tag="VF516_33025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112616.1"
                     /translation="MGSVTRVLMAVLLLAPVGLAAAPPDADPSGHWEGTIQAPNRSVA
                     FEIDLGKNARGELIGTFGSPAQGEKGLPLGTIAVTGREVRFAVNATSGGGMFHATLRD
                     DGRAMAGDFTLARGGQSVPFQLTRTGDARFTPPPTSPPISKRLEGRWTGTLTVDGKHL
                     TIGLAMANQPDGTATGTVRSDGLQLPIAIAERGARLRIEVPSVGASFAGEIDAAGTRL
                     VGTWSQRTLSLPLTFARDAK"
     gene            3695..3841
                     /locus_tag="VF516_33030"
     CDS             3695..3841
                     /locus_tag="VF516_33030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112617.1"
                     /translation="MKQTTQADAKPKKLVIHKDSVRTLASDAMKQVVAAGILTPCLRC
                     GTTR"
     gene            complement(3843..>4978)
                     /locus_tag="VF516_33035"
     CDS             complement(3843..>4978)
                     /locus_tag="VF516_33035"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEX8112618.1"
                     /translation="TPGGPGLAGGAAGMAVCAAYVDDAATRRLWQVAADALENTALPP
                     GLFDGVTGIGWATAHLRDRLFEDGDDPCEAVDDFVLDRVSHPGFGRPFDLGAGLVGFG
                     IYALERLPVPACAQILAHIVERLGEIVEPAGPAGCHLALRTPPRELIPLRRAQFPDGW
                     FNLGLAHGVPGVIALLAGIRAAAVRPAETTHLLDALVGWLLAQARPDAPARYAHWVGD
                     AEPDRTQLAWCYGDLGIAAALHHAGVACNRPSWCGEALELARAAAQRSIETIPGIDAG
                     LCHGAIGAAHGFARLHHLTGDGSLRQAAADWYSHALALRTPGRGLAGWRAYQGPGNGG
                     DDEWISDAGLLSGAAGIALSLHAATTAREPSWDRALLWSVRGC"
BASE COUNT          687 a         1767 c         1838 g          686 t
ORIGIN      
        1 gttcggcggc gaggtgtcgt ccaacgccgc cgacgcgttc gccgccggcc tcaagggccg
       61 ctacaaggag gaccgctacg acgagcacaa gctggtcggc tcgcacttcg cgatggacgt
      121 gctcgagggc ggcaaggtcg ttcacaagga catcccgatg cgcaacgcgc tcaacgaggt
      181 gctgcgcacc gagtacatca aggactgcca gcgcggcgtc gaccgctgga acaagacgat
      241 caaggacgag ggcatcgact tcgagctgcg cctgccgtcg aacaagttcc accgccagat
      301 cggcatgtgg gccggccagg gattcacccc cgacggcgag atgctcgacg ccgccgcgtt
      361 cgccgcgcgg cgcggcgagt ggctgcccaa cgacgccgac gaggcgtaca tcaagagcct
      421 gatgcacgcg gtctacgagc ccggcaagat ggcgagctgg atcgcgccgc cgatgcgcgg
      481 catcaacggc ctgcccggcg agttcgtcta cgtccggatg tagccggccg cggccggtga
      541 tcgcccggtc gaacgccggg tgatccaggc ccggcgcgga cttgcgcggg gccgcgccgc
      601 gcccgagggg atcgtcttgc gcgacagtcc tactgtgcta tctcagtagt acagttgacg
      661 gcgatgctgc agcatgctgt tccggcccaa cccctcgtcc ggcgttccga tctacctcca
      721 gctgatggag caggtgaagc acgccatcga gaccggcgcg cttcgcccgg gcgaacagct
      781 gccgggcatt cgcccgctgg cggaggagct cgtgatcaat cccaacaccg tggccaaggc
      841 ctaccgcgag ctcgagcacg agggcgtgat cgagctgcgt cacggcgccg gcgcattcgt
      901 cgccgccggc ccgcgcgcca agaaggcgac cgacaacctg cgcgccggcc aggcgctggt
      961 cgcgtcgacc gtcgagcggc tgcgcgcgcg cggcctgagc gacgacgaga tccggcggct
     1021 gttcgaggcc gagctcgccg gccgggccgc cgcgccgcgg acgggaggcc agcgtggctg
     1081 aaccgctcgt catcgagacc gcggggctgt gcaagcgcta cggtgagccc gaaggcgggc
     1141 gccaaggcgg aatcgacgcg ctgcgcgggc tcgacctcca ggtcccggcc gggtcgatct
     1201 gcggcttcct cggacgcaac ggcgccggca agacgacgac gatcaaggtg ctgctcggga
     1261 tggcccacgc gagcggcggg cacgcccggg tgttcggcct gccggtgggc gacgagcgcg
     1321 ccagcgtcga gatccggcgc cgcaccgggt tcgtcagcga cgacaaggac ctctacgact
     1381 acatggacgt cgcggggatg atccggttca ccgccgcgtt ctacccgcgc tggcgcgccg
     1441 acctcgcgcg cgactacctg cggcggttcg agctcccgcc ggatcgcaag gtcaagtcgc
     1501 tgtcgcgcgg catgcggacc aagctcgcgc tgctggtcgc gctgtgccgc ggcgccgagc
     1561 tgctgatcct cgacgagccg accgcggggc tcgaccccgc gatggccgag caggtgctgc
     1621 aggcgctggt cgcgcacgtc gcgcgcgagg cgaccacggt gttcttctcg tcgcaccaga
     1681 tggccgaggt cgatcagatc gccgatcgca tcgcgatcat cgatcgcggc cgcgcggtgc
     1741 tgtccggcgc gctcgatgac ctgcgggcac ggttccgccg catccagctg gtgttcgacc
     1801 gcgacccgcc ggagatcgtg ctgcgcgcgc ccgtggtggg ccgggtgcgg cgccagggcc
     1861 gggtggtcag cgtgctgacc agcgcgggcg ccgatccgat cctcgacgag gttcgcgcgc
     1921 tcggcccggt ctccgtcgac gtcgccccgg tcggcctgaa ggagatcttt ctcgagaccg
     1981 ttgccgcgga ggattgacat gctctggcac aagtcgtggc tcgaaacccg ctggcgcttc
     2041 ctgatcggcc tcggcctgct ggtgtgctcg gcggccggcg tcgtgctcgc gtaccccagg
     2101 gtcgtcgagc tgctcgcgct cgtgccggcg cgcgacaccg gcggcgagct cggccggcgg
     2161 atccgcgagt cggcagagct ggcgcgcgac taccgcggct acgtgtggtc gcaatggttc
     2221 ggccagcacc tggtccaggc ctggacgctg ttcgcggtgc tgctcggcac cggcggactg
     2281 gtctcgcagg cggtccgggg cggcgcgctg ttcaccctgt cgctcccggt gtcgcgccgc
     2341 cgcctggtcg ccgtgcgggc cgcggtcggt ctcgccgagc tgctggtgct cgcggtcgtg
     2401 ccgtcgctgc tgatcgcggc gctgtcgccg gcgatcggcc agcgctacgg cgtcgcggac
     2461 gcgctggtcc acggcgcgtg cctgttcgtc gccggcaccg tgttcttcag cctcacgtcg
     2521 ctgctgtcca ccgcgttcag tgacatctgg cgtccgccgt tgatcgtgct gctcgtcgcg
     2581 gtcgtgctcg gtgtctcgga gcagctggtc cgcgacgtcg cgcggttcag cgtgttctcg
     2641 gtgatgagcg cgcagcgcta cttccagggt ggtgggttgc cgtggctggg ccttgccgcc
     2701 agcgcggctg tctcggtggc gctgctgtac ggcgccgccc gcaacgtcgc gcgccaggac
     2761 ttctgacgcg caaaggagaa cgacatgggc tctgtcactc gcgtgttgat ggctgtgctg
     2821 ttgcttgcgc cggtcggtct ggccgccgcg ccacccgatg ccgacccgtc cggccactgg
     2881 gagggaacga tccaggcgcc gaaccggtcc gtggcgttcg agatcgacct gggcaagaac
     2941 gcccggggcg agctgatcgg cacgttcggc agcccggcgc agggcgagaa ggggctgccg
     3001 ctcgggacga tcgccgtcac cggccgcgag gttcgcttcg cggtcaacgc gaccagcggc
     3061 ggcggcatgt tccacgccac cctgcgcgac gatggccggg cgatggcggg cgacttcacg
     3121 ctcgcgcgcg gcggccagtc ggtcccgttc cagctgaccc ggaccggcga cgcccggttc
     3181 acgccaccgc cgaccagccc gccgatcagc aagcggctcg aaggccgctg gaccggcacg
     3241 ctcaccgtcg atggcaagca cctgacgatc ggcctggcga tggcgaacca gcccgacggc
     3301 accgccaccg gcacggtccg cagcgatggc ctgcagctgc cgatcgcgat cgccgagcgc
     3361 ggcgcgcgcc tgcggatcga ggtgccctcg gtcggcgcct cgttcgccgg cgagatcgac
     3421 gccgccggca cgcggctggt cggcacctgg agccagcgca cgctgtcgct gccgctgacg
     3481 ttcgcacgcg acgcgaagta ggtcgccaga tcgggcgacg ggccgccgcg tggcatcgag
     3541 cgcagcgaga cggggggtgg ggaccccgac ggctccgccg gcggggcggg gggcagagcc
     3601 cccgtgggat cgatcacgac gccgcgcgtc gggcggtggg ggcctgcttg accggctctg
     3661 caacgatctg atgtagtcga cacaggagtc catcgtgaag caaaccacgc aggcagatgc
     3721 caagcccaag aagctcgtga tccacaagga cagcgtgcgg acgctcgcga gcgatgcgat
     3781 gaagcaggtc gtcgcggcgg ggatcctcac gccgtgcctt cgctgcggca cgacgcgctg
     3841 agctagcagc cccgcaccga ccacaggagc gcgcggtccc aggatggttc gcgcgctgtg
     3901 gtcgccgcat gaagtgacag ggcgatcccg gcggcgccgg acagcaggcc ggcatcgctg
     3961 atccattcat cgtcgccgcc gttgcccggt ccctggtagg cgcgccaacc ggcgagtccg
     4021 cggccaggcg tgcgcagcgc gagcgcatgg ctgtaccagt ccgccgcggc ctgccggaga
     4081 ctgccatcgc cggtcagatg atggagccgc gcgaagccgt gcgccgcgcc gatcgcgccg
     4141 tggcacagcc cggcgtcgat cccggggatc gtctcgatcg agcgctgcgc ggcggcgcgg
     4201 gcgagctcca gcgcctcgcc gcaccaggac gggcggttgc aggccacgcc ggcgtggtga
     4261 agtgccgccg cgatgccgag atcgccatag caccacgcga gctgggtgcg atcgggctcg
     4321 gcgtcgccga cccagtgggc atagcgcgcc ggggcgtcgg gccgggcctg ggcgagcagc
     4381 cagccgacca gcgcatcgag caggtgcgtg gtctccgcgg gccgcaccgc cgcggcgcga
     4441 atcccggcga gcagcgcgat cacgccgggc acgccgtggg cgagcccgag gttgaaccaa
     4501 ccgtcgggaa actgcgcgcg gcgtagcggg atcagctcgc gcggcggcgt gcgaagcgcg
     4561 aggtggcacc cggcgggacc ggcgggctcg acgatctcac caagccgctc gacgatgtgc
     4621 gccaggatct gcgcgcacgc cgggacgggc agccgctcca gcgcgtagat cccgaacccg
     4681 accaggcccg cgccgaggtc gaatggccgg ccgaatcccg gatggctcac gcgatcgagg
     4741 acgaagtcat cgaccgcctc gcatggatcg tcgccgtcct cgaacagccg gtcgcgcagg
     4801 tgcgcggtcg cccagccgat gccggtgacg ccatcgaaca ggcccggcgg cagcgcggtg
     4861 ttctcgagcg cgtcggccgc cacctgccac agccgccggg tggcggcgtc gtcgacgtac
     4921 gcggcgcaca ccgccatgcc cgcggcgccg ccggccagac ccgggccgcc cggggtcg
//
LOCUS       DASVOR010001776         2083 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_486082,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001776 DASVOR010000000
VERSION     DASVOR010001776.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2083
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486082"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VF516_33040"
     CDS             complement(<1..312)
                     /locus_tag="VF516_33040"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEX8112619.1"
                     /translation="MTALLAVDVGNTNTVLGVFRDGELAAHWRIQTVAERTADEYAVL
                     LKTLLDMNGFPWRSINAGIISSVVPPTLFGLQQFFKRHCGGPALVVGAGIKSGMPILY
                     EN"
     gene            367..1158
                     /locus_tag="VF516_33045"
     CDS             367..1158
                     /locus_tag="VF516_33045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112620.1"
                     /translation="MRPPSKILASAGLAASLALGALGALGALGAALAACGGGSDSPPG
                     PLAKHFDDMYIAAIPPAQKPSVVQTQNDWSVARMENAKAEADYNESATQLSVAQNDQK
                     AAHLGVESAVANKKAAEASADTNRVNDATKNLHTAEDLAKAADARVKYIEAYRNYLQV
                     AMRYAQENVYWREAQYEVAKSQVGQGNHIAPKGVEFDAFPKQEQERRKRTDAAKERLD
                     TEKGRVQSVRENWMKAQETADREYGRPANFPDPMAARSGSSGSSL"
     gene            1161..>2083
                     /locus_tag="VF516_33050"
     CDS             1161..>2083
                     /locus_tag="VF516_33050"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112621.1"
                     /translation="MGRPDDGTRTTAPGTSVTAESGDAAHILVVDDERVIREILAEFL
                     TLEGFSVHTVEDGEKALTELRLRPYDLLITDLKMPRVSGLQLLERIEAERLGVLTVLM
                     TGFGTVETAIEAMKKGAYDYLLKPFKVEEVIHVVRRALYRQRLQAENIRLREALTIYR
                     VSEAIASSRDIEHVLDVVLRAAIDEVKADVATLHLRDARTGGYEERVKVVAFDGAPRG
                     LPSPAFALLTDQFAQGAPILAHGGRAGRFFTEDAAPADLASFVSVPLQVRSNMVGVLN
                     VFSFTPGKKFDEGHRKMLSVLASRAASAIDNT"
BASE COUNT          348 a          747 c          713 g          275 t
ORIGIN      
        1 gttctcgtac aggatcggca tcccgctctt gatcccggcg ccgaccacca gcgcgggccc
       61 gccgcagtgg cgcttgaaga actgctgcaa gccgaacagc gtcggcggaa ccaccgacga
      121 gatgatcccg gcgttgatcg accgccacgg gaagccgttc atgtcgagca gggtcttgag
      181 cagcaccgcg tactcgtcgg cggtgcgctc ggcgacggtc tggatccgcc agtgcgccgc
      241 gagctcgcca tcgcgaaaca ccccgagcac ggtgttcgta ttcccgacat cgacggcaag
      301 cagcgcggtc atcgcccggt aatggtagct cgccgtgggc acccgtagta ggctggccac
      361 ctcgctatgc gcccaccctc gaagatcctc gcgtccgccg ggctggcggc gtcactagcc
      421 ctcggggccc tcggggccct cggggccctc ggggccgcgc tcgccgcgtg cggcggcggc
      481 tcggactcgc cgcccggccc gctcgccaag cacttcgacg acatgtacat cgcggcgatc
      541 ccgccggccc agaagccgtc ggtggtgcag acccagaacg actggtcggt ggcgcggatg
      601 gagaacgcca aggccgaggc cgactacaac gagtccgcga cccagctgtc ggtcgcgcag
      661 aacgaccaga aggcggccca cctcggggtc gagtccgcgg tcgccaacaa gaaggcggcc
      721 gaggcgtcgg ccgacaccaa ccgggtcaac gacgcgacca agaacctgca caccgccgag
      781 gacctcgcca aggcggccga cgcgcgggtc aagtacatcg aggcctatcg caactacctg
      841 caggtcgcga tgcgctacgc gcaggagaac gtgtactggc gcgaggccca gtacgaggtc
      901 gccaagtccc aggtcggcca gggcaaccac atcgcgccca agggcgtcga gttcgacgcg
      961 ttccccaagc aggagcagga gcgccgcaag cgcaccgacg ccgccaagga gcggctcgac
     1021 accgagaagg gccgcgtcca gagcgtgcgc gagaactgga tgaaggcgca ggagaccgcg
     1081 gatcgcgagt acggccgccc cgccaacttc cccgatccga tggcggccag gtccgggtcc
     1141 tcgggctcgt cgctgtaggg atgggccggc ccgacgatgg gacccgtacg acggcgcccg
     1201 gcaccagcgt gacggccgag tccggagatg ccgcgcacat cctcgtcgtc gacgacgagc
     1261 gcgtgatccg cgagatcctc gccgagttcc tcacgctcga gggcttctcg gtccacaccg
     1321 tcgaggacgg cgagaaggcg ctaaccgagc tccggctgag gccctacgac ctcctgatca
     1381 ccgacctcaa gatgccgcgg gtgtccgggc tccagctgct cgagcggatc gaggccgagc
     1441 ggctcggcgt gctcaccgtg ctgatgaccg ggttcggcac cgtcgagacc gcgatcgagg
     1501 cgatgaagaa gggcgcctac gactacctgc tcaagccgtt caaggtcgag gaggtgatcc
     1561 acgtcgtgcg ccgcgcgctg taccgccagc gcctgcaggc cgagaacatc cggctgcgcg
     1621 aggcgctgac gatctaccgg gtgtccgagg cgatcgcctc gtcgcgcgac atcgagcacg
     1681 tgctcgacgt cgtgctgcgc gcggcgatcg acgaggtcaa ggccgacgtc gcgaccctcc
     1741 acctgcgcga cgcgcgcacc ggcggctacg aggagcgcgt caaggtggtc gcgttcgacg
     1801 gcgcgccgcg cggcctgccg tcgccggcgt tcgcgctgct caccgaccag ttcgcccagg
     1861 gcgcgccgat cctcgcccac ggtggccgcg ccgggcggtt cttcaccgag gacgccgccc
     1921 ccgccgacct ggcgtcgttc gtgtcggtgc cgctccaggt ccgcagcaac atggtcggcg
     1981 tgctcaacgt gttctcgttc acgccgggca agaagttcga cgagggccac cgcaagatgc
     2041 tgtcggtgct cgcgtcgcgc gccgccagcg cgatcgacaa cac
//
LOCUS       DASVOR010001777         4016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_164226,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001777 DASVOR010000000
VERSION     DASVOR010001777.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164226"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VF516_33055"
     CDS             complement(<1..1183)
                     /locus_tag="VF516_33055"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112622.1"
                     /translation="MSNEKPSQPSRPAGQPGELLRPDPARAATVDSTEPFERVEAADP
                     TELPTTDIDSEPDLEADQLLRAVAYSPPRLPRVAMAPGTRWGNGDRYTIERRLGRGGM
                     GTVYAATDTVLQRVVALKVLDAVEPAQQASHHARLLREAQLAARVEHERVARVYDVGA
                     HDGFAFVAMEYVPGGTLRQWMTGREVPWPQVVDIATQIAEGLAELHSRGIVHRDLKPE
                     NAMLTAQGGVKLLDFGLARHAVVALDEPPGAPVRPDAADGTTVATCGTPGYMAPEQYG
                     NQPLDARIDVFALGVVIFELATGQRLFVGTTPREIMNATLAGAPPLTGGVWASAPERL
                     RDHIRRMLAVDPKQRFANGIQVLAALRALAPALPPPRSGLPAATLTSRDLTQPDLRPS
                     RA"
     gene            complement(1376..2005)
                     /locus_tag="VF516_33060"
     CDS             complement(1376..2005)
                     /locus_tag="VF516_33060"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8112623.1"
                     /translation="MVSYRPVHQVPASSDLDHRARELLRAGDTRGTATLVLRELGPEV
                     LGFLSGVLGEADADEVYSAWSERLWRSLKGFEGRCSLRTWTYVLARREISRFRKGMRR
                     HAEGRVPLSQLQDVLAVPKSRTRTTMATAKQQQLTALRDELPIEDRTLLILRVDRKLS
                     FDEIALAFAENPESVTEVDRRREAARLRKRFQLVKQRLVARARRHAAAS"
     gene            2070..2591
                     /locus_tag="VF516_33065"
     CDS             2070..2591
                     /locus_tag="VF516_33065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112624.1"
                     /translation="MADHILPARRMVSIFRRTAGRGTQVEPWSVHRGQKPQKKTTARF
                     HIRRDARTTSAKERGTRPSHGTGARVVCYECGNENASMNLVCAYCGFPFARDHIAAGR
                     REPVESEPIRFAVLVVGLVLALVVPVVGLVAGFSAMRRSDPAHQGASRRWLVAALCSC
                     LAYIALLMGYLVL"
     gene            complement(2822..3595)
                     /locus_tag="VF516_33070"
     CDS             complement(2822..3595)
                     /locus_tag="VF516_33070"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEX8112625.1"
                     /translation="MLRSGFVMVVIVLTTQTARAQIVNVQGALAKPPASDGATGTVEL
                     KLNWREGNNPLFDVGGAGSVVGRLGNVLGLVLARGEYGTSRGLTLTRKSFEHARTRIE
                     LGPRWRWEAFAQHEYDQFRRLSLRALAGTGPALQIVDDPAIALLAGAAYLFEYERLDR
                     RAGTIDAGLRATAHRASLYLTGHEEPGAGVLIVETVYAQPRLDHPGDLRMLGELSVLS
                     KLASRVALKNAFTVAYDRTPPDGVRRTDTQLEVAIVVTF"
     gene            3753..>4016
                     /locus_tag="VF516_33075"
     CDS             3753..>4016
                     /locus_tag="VF516_33075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112626.1"
                     /translation="MPFVLLLALGSPGLIGRAAAQPAGNLTLDAFRPAIDSRGYLTVN
                     ASQVLGDRELSFGLGSLDWGHHLLSLDAGGKSYSIDDMITATLI"
BASE COUNT          524 a         1359 c         1510 g          623 t
ORIGIN      
        1 gcgcgcggct cggccgcagg tcgggctggg tcaggtcccg gctggtcagg gtcgcggccg
       61 gcaaccccga gcgcgggggc ggcagcgcgg gcgcgagcgc gcggagcgcg gcgagcacct
      121 ggatcccgtt ggcgaaccgc tgcttcggat cgaccgcgag catccggcgg atgtgatcgc
      181 ggagccgctc cggggcgctc gcccacacgc cgccggtcag cggcggcgca ccggcgaggg
      241 tcgcgttcat gatctcgcgc ggcgtcgtgc cgacgaacag ccgctggccg gtggcgagct
      301 cgaagatgac gacgccgagc gcgaacacgt cgatccgcgc gtcgagcggc tggttgccgt
      361 actgctccgg cgccatgtaa ccgggcgtgc cgcaggtcgc gacggtggtc ccgtcggcgg
      421 cgtccgggcg gaccggcgcg ccggggggct cgtcgagcgc gaccacggcg tggcgggcga
      481 gcccgaagtc gagcagcttg accccgcctt gtgccgtcag cattgcgttc tcgggcttga
      541 gatcgcggtg gacgatgccg cggctgtgga gctcggcgag gccctcggcg atctgggtcg
      601 cgatgtcgac gacctgcggc cacggcacct cgcgcccggt catccactgc cgcagcgtgc
      661 cgccggggac gtactccatc gcgacgaacg cgaagccgtc gtgggcgccg acgtcgtaga
      721 cccgcgcgac ccgctcgtgc tcgacgcggg ccgcgagctg ggcctcgcgg agcagccgcg
      781 cgtggtgcga cgcctgctgc gcgggttcga ccgcgtcgag caccttgagc gccacgaccc
      841 gctggagcac ggtatcggtc gcggcgtaca ccgtgcccat cccgccgcgg ccgagccggc
      901 gctcgatcgt gtagcgatcg ccgttgcccc accgcgtgcc cggcgccatc gcgactcgcg
      961 gcaggcgcgg cggcgagtac gcgaccgcgc gcagcagctg atcggcctcg aggtcgggct
     1021 cgctgtcgat gtcggtggtc ggcagctcgg tcggatcggc ggcctcgacc ctctcgaacg
     1081 gctcggtcga gtcgaccgtg gccgcgcggg ccggatcggg ccgcaaaagc tcgcccggct
     1141 gtcccgccgg ccggctcggt tgtgacggct tctcgttcga cattccgtgc tcggacccct
     1201 gaaacacccg ccccgcgtgg ctcatcgttt ccccgtggcc tggctcccca gacagaaccc
     1261 tcggaggcaa caatgtgaag gtcgagcgta cgatttttgg acgcttctcg ccaaccgtct
     1321 gaaatgcgcg gccgcggttg gcccagaagt aagcgttctc gggcttattt cagcgctagc
     1381 tggcggcggc gtggcggcgc gcccgggcga cgagccgctg cttgaccagc tggaaccgct
     1441 tgcgcagccg ggcggcctcg cgcctccggt cgacctccgt gaccgactcg gggttctcgg
     1501 cgaacgccag cgcgatctca tcgaagctga gcttgcggtc gacgcgcagg atcagcaggg
     1561 tgcggtcctc gatcgggagc tcgtcgcgca gcgccgtgag ctgctgctgc ttggcggtcg
     1621 ccatggtggt gcgcgtccgg gacttgggga ccgcgaggac gtcctgtagc tgggacagcg
     1681 ggacccggcc ctcggcgtgc cgccgcatgc ccttgcggaa ccggctgatc tcgcggcgag
     1741 ccaggacgta ggtccatgtg cggagcgagc agcggccctc gaagcccttg agcgatcgcc
     1801 acagccgctc gctccacgcc gagtagacct catcggcgtc ggcctccccg agcacgccgg
     1861 acagaaagcc gaggacctcg gggccgagct cgcggagcac cagcgtcgcc gtcccacggg
     1921 tgtccccggc gcgaaggagc tcgcgggccc ggtgatcgag gtcgctgctg gccggcacct
     1981 ggtggaccgg acggtaggat accaagctgc gttcatcaga caactccgcg ggccgccgtc
     2041 cgccgctccg cggcaagccg cgtgatgccg tggccgacca catcctgcca gctcggcgga
     2101 tggtcagcat ttttcggagg actgccggcc gcggaacgca ggtcgagccg tggtcggtcc
     2161 accgcgggca gaagccgcaa aaaaaaacca ctgcgcggtt tcacatccgg cgcgacgcgc
     2221 gcactacctc ggcaaaggag cgaggtaccc ggccatcgca cggaacagga gctcgtgtgg
     2281 tttgctacga gtgcggtaac gagaatgcct cgatgaatct ggtctgtgca tactgcggat
     2341 ttccgtttgc cagagatcac atcgcggcgg ggcggcgcga gccagtcgag agcgagccga
     2401 tcaggttcgc cgtgctcgtg gtcgggcttg tgctcgctct ggtcgtaccg gtggtcgggc
     2461 tggtcgcggg gttctcggcg atgcgccggt cggacccggc tcaccagggg gcgagccggc
     2521 ggtggctggt cgcggcgctg tgctcctgtc tggcatacat cgctctgctg atggggtatc
     2581 tcgtattgtg aggtttgggg tcatccgttg agcagcttcg tttgggggag tccgttgccg
     2641 gcgctgcggg gccgggtggg tggtcgaggg cgccgtggga tgggggcggc agcgtcggca
     2701 acggacactg agtggcttgg ttcgagtctg gggagtccgt ttccggcgct gcgggggccg
     2761 ggtgggtggt cgagggcgcc gtgggatggg ggcggcagcg tcggcaacgg acactggggg
     2821 ttcagaaggt cacgacgatc gcgacctcga gctgggtgtc ggtgcgccgg acgccgtcgg
     2881 gcggggtccg atcataggcg acggtgaagg cgttcttgag cgcgacccgc gacgcgagct
     2941 tgctcaggac cgagagctcg ccgagcatgc gcaggtcgcc cgggtgatcg agccgcggct
     3001 gggcgtagac cgtctcgacg atcagtacgc cggcgccggg ctcctcgtgg ccggtcaggt
     3061 agagcgaggc gcggtgcgcg gtggcgcgca ggccggcgtc gatcgtgccg gcccggcggt
     3121 cgagccgctc gtactcgaac aggtacgccg cgccggcgag cagcgcgatc gccgggtcgt
     3181 cgacgatctg cagcgcgggg ccggtgccgg cgagcgcgcg caggctgagc cgccggaact
     3241 gatcgtactc gtgctgggcg aacgcttccc accgccacct ggggccgagc tcgatccggg
     3301 tgcgggcgtg ctcgaacgac ttcctggtca gggtgaggcc gcggctcgta ccgtactcgc
     3361 cgcgcgcgag cacgaggccg agcacgttgc cgagccggcc gaccacgctg ccggcgccgc
     3421 cgacgtcgaa cagcgggttg ttgccctccc gccagttgag cttgagctcg accgtgccgg
     3481 tcgcgccgtc gctggccggc ggcttggcga gcgcgccctg cacgttgacg atctgggccc
     3541 gcgccgtctg ggtggtgagc acgatcacga ccatcacgaa cccgctgcgc agcatgggcc
     3601 gcgcatcgta accgctccgc caacttgcgt ccgcgcgcga gcgatgtaac cggcaggtgg
     3661 aatccgcgca cagtggttct ggctgattcc ggcggcgcgt ttcccggagt cggcgaagga
     3721 ccgtcatgcc acgcctggct catggccgcc gtttgccctt cgtgctcttg ctcgcgctcg
     3781 ggtcgcccgg gctgatcggg cgcgccgccg cccagccggc cggcaacctg acgctcgatg
     3841 cgttccgccc ggcgatcgac tcgcgcggct acctcacggt caacgcgtcg caggtgctcg
     3901 gcgaccgcga gctgtcgttc gggctgggct cgctcgactg gggccaccac ctgctgtcgc
     3961 tcgacgccgg cggcaagtcg tactcgatcg acgacatgat caccgcgacg ctgatc
//
LOCUS       DASVOR010001778         1746 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_280443,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001778 DASVOR010000000
VERSION     DASVOR010001778.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280443"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1614
                     /locus_tag="VF516_33080"
     CDS             <1..1614
                     /locus_tag="VF516_33080"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEX8112627.1"
                     /translation="PAAQPAPAADPYAAIDGAGKAITADFLREQITKISSDEFEGRGP
                     MTHGDQAARAYLASQLQAMGYEPGDGKTWEQPVELVGMKAEMPAKWTFRHGGKSLALS
                     WMDQYIGRAGVQTEKVEINNAELVFVGYGIQAPEYGWDDFKGKDLKGKVLVMMNNDPD
                     WDPALFAGTTRLYYGRWDYKYESAARQGAAGAIILHTTPSAGYPFQVVQTSWSGETFE
                     LPAAGEPRIQVKAWASEDAVRALVALAGRDLSALIESARHKEFTPVPLGITTSLRFTN
                     QIHRGKSANVWGLLRGSDPVLGQELVVFSAHHDHLGIRKPDATGDKIYNGALDNASGC
                     SQILAIAKAFKALPTPPRRSVLFLFVAGEEQGLLGSLYYATHPTVPTGKIAADINYDG
                     GDIWGKTRDVTYVGKGKTTLDPLVDEFAARQGRVVKPDQFPDRGAFYRSDQFSFARVG
                     VPGLYLHTGTDFIGRPAGWGREQIEAYEKTRYHQASDQILPDWNFDGMILDAQLGFAV
                     GVKVANADPLPGWVPGDEFEAARKKSLQH"
BASE COUNT          288 a          648 c          570 g          240 t
ORIGIN      
        1 ccggccgcgc agcccgcccc ggccgccgac ccgtacgccg cgatcgacgg cgccggcaag
       61 gcgatcaccg ccgatttcct gcgcgagcag atcaccaaga tctcgtccga cgagttcgag
      121 ggccgcggcc cgatgaccca cggcgaccag gcggcgcggg cctacctggc ttcccagctc
      181 caggccatgg gctacgagcc cggcgacggc aagacctggg agcagccggt cgagctggtc
      241 ggcatgaagg ccgagatgcc ggccaagtgg accttccgcc acggcggcaa gtcgctcgcg
      301 ctcagctgga tggatcagta catcggacgc gccggcgtcc agaccgagaa ggtcgagatc
      361 aacaacgccg agctcgtctt cgtcggctac ggcatccagg cgcccgagta cggctgggac
      421 gacttcaagg gcaaggacct caagggcaag gtcctcgtga tgatgaacaa cgatccggac
      481 tgggatccgg cgctgttcgc cggcaccacc cgcctctact acggccggtg ggactacaag
      541 tacgagagcg cggcgcgcca gggcgcggcc ggtgcgatca tcctccacac cacgccgtcg
      601 gccggctacc cgttccaggt cgtccagacc tcgtggtcgg gcgagacctt cgagctgccc
      661 gctgccggcg agccgcgcat ccaggtcaag gcgtgggcca gcgaggacgc ggtgcgcgcg
      721 ctggtcgccc tggccggccg cgacctgtcc gcgctgatcg agtcggcgcg gcacaaggag
      781 ttcaccccgg tgccgctcgg catcaccacc tcgctccgct tcaccaacca gatccaccgc
      841 ggcaagagcg ccaacgtctg gggcctcctg cgcggctccg atccggtcct cggccaggag
      901 ctcgtcgtgt tctcggcgca ccacgaccac ctcggcatcc gcaagcccga cgccaccggc
      961 gacaagatct acaacggcgc gctcgacaac gcctcgggct gctcccagat ccttgcgatc
     1021 gccaaggcgt tcaaggcgct gccgaccccg ccgcgccgct cggtgctgtt cctgttcgtc
     1081 gccggcgagg agcagggcct gctcggctcg ctgtactacg cgacccaccc gaccgtgccg
     1141 accggcaaga tcgccgccga catcaactac gacggcggcg acatctgggg caagacccgc
     1201 gacgtgacct acgtcggcaa gggcaagacc acgctcgatc cgctggtcga cgagttcgcc
     1261 gcgcgccagg gccgcgtcgt caagcccgac cagttccccg accgcggcgc gttctaccgc
     1321 tccgatcagt tcagcttcgc ccgggtcggc gtccccggcc tctacctcca caccggcacc
     1381 gacttcatcg gccgcccggc cggctggggc cgcgagcaga tcgaggccta cgagaagacg
     1441 cgctaccacc aggccagcga tcagatcctg cccgactgga acttcgacgg catgatcctc
     1501 gacgcacagc tcggcttcgc ggtcggcgtc aaggtcgcca acgccgaccc gcttcccggc
     1561 tgggtgccgg gcgacgagtt cgaggctgcg cgcaagaagt cgctgcagca ctgacgctgc
     1621 gccgctcagg ccatctcgac gctgggcagg aagcgcggcg ggacgcggtg cttggtcttc
     1681 tgctcgtagg cgaacgcgta gccgagcagc ttgccctcgg tgtagggctt gccgaagaac
     1741 gacagg
//
LOCUS       DASVOR010001779         4074 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_135158,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001779 DASVOR010000000
VERSION     DASVOR010001779.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4074
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135158"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VF516_33085"
     CDS             complement(<1..897)
                     /locus_tag="VF516_33085"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8112628.1"
                     /translation="MRMAVDVSVVRDMAALRAIEAEWRALASVGGSGLFRGPDWLIPW
                     WQAYHATLSAELYVLVGRAAEAEPSGAAAGDIVCIAPLYRRTVKVALLDTRELRMIGD
                     AGPRPPALDLLARAGWEDRAGIALARKLIDEAATWDLIDLEPLADPSRVRAHLVQRLA
                     PFGFTVESAPSAGGATRIALALAPIEATDVSRVVTTYGDDLAALRKGMTALRRLSRLE
                     WAERDEPSPLADPEAIGLLEQVAMELGKHGRLRLARIDDAPGEACAVALVVDDGDRAV
                     VLAMAVDPQAGRASARLLHAEAV"
     gene            complement(1030..2472)
                     /locus_tag="VF516_33090"
     CDS             complement(1030..2472)
                     /locus_tag="VF516_33090"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEX8112629.1"
                     /translation="MPDLDRIADRKLAAHVVAGGRLCALVLELWQRTRMDWGFVTDRL
                     ATAFRKESWIGSHERRFVSETLYGLVRHLRRIDAGLARGRRTERAPRDLERLLALFVL
                     DGLIDPARAAATCGELDWNAVAEIDQVIARERKLVPRIALAASLPDWLAARLAADWGD
                     EAEALALALNRRAPMTVRANLLIGDRTALAAELARDRIATLPGRWCDTALHLESRTNL
                     FALAAFTRGAMEAQDEGSQLLADLAAACARGTAGAPGLVDQEGGFPIPPLWGLARQSR
                     AIHPSVRRDARLIIDLCAGAGGKTLAIAARLGNRGRILSADIDARKLDELRRRARRAR
                     VSNAQTVHLDGGRWPAQLDALRGKADVVLVDAPCSGIGALRRNPEARWRLREADLAEF
                     AARQQEILGGARTLLAPGGRLVYATCTLLAIENADVVAAVVGPGLATVPVREILGDRA
                     RELGDGDAFTMTPHRHGTDGFYARVMRRVD"
     gene            2575..3891
                     /locus_tag="VF516_33095"
     CDS             2575..3891
                     /locus_tag="VF516_33095"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEX8112630.1"
                     /translation="MSTETKPLPTVKEGGVTSLKERGNLFEIATRQIDKAMKLINIDP
                     DIAQILAQPKNELIVNFPVRMDNGKFQMFKGYRVQHNNILGPYKGGMRYHHEANLDEM
                     KALASWMTYKSALHEIPFGGGKGGIKVDPRQHSKAELERITRRFTAALGSNIGPEYDI
                     PAPDVNTNSQTMVWMMDTYMNIVGAGDRNTVRGVVTGKSVSSGGSHGRAEATGQGVVH
                     CITEWARDKNFNLDGCLVIIQGFGNVGSYAARLLSQKGAVIVGVGDAGGYIANPEGIN
                     PHKLGEHVHKTGTVAGYKGAQKIERAEFFALDAHIFVPAALELELGVAEAKALKVKLV
                     VEGANGPTYPEAEDVLLGKGIDIIPDILCNSGGVIVSYYEWLQNKRAEQWDVEDVLDR
                     LERRMKQTWANVRHYVAVNKTDWRTASYAIGLERLQHCYRERGIFP"
BASE COUNT          639 a         1502 c         1425 g          508 t
ORIGIN      
        1 gaccgcctcg gcgtggagca gccgggcgga ggcgcggccg gcctgggggt cgaccgccat
       61 cgcgagcacg acggcgcgat cgccgtcgtc gacgaccagc gccaccgcgc aggcctcgcc
      121 cggggcgtcg tcgatccgcg ccagccggag ccggccgtgc ttgccgagct ccatcgcgac
      181 ctgctcgagc aggccgatcg cctcgggatc ggcgagcggc gacggctcgt cgcgctcggc
      241 ccactcgagg cgggacagcc ggcgcagcgc ggtcatgccc ttgcgcagcg ccgccaggtc
      301 gtcgccgtag gtggtcacca cgcgcgaaac gtcggtggcc tcgatcggcg cgagcgccag
      361 cgcgatccgg gtcgcgccgc cggccgacgg cgcgctctcg acggtgaacc cgaacggcgc
      421 gagccgctgg accaggtggg cgcgcacccg cgacgggtcg gcgagcggct cgaggtcgat
      481 caggtcccag gtcgcggcct cgtcgatcag cttgcgggcg agcgcgatcc ccgcgcgatc
      541 ctcccagccg gcgcgggcga gcagatcgag cgcgggcggc cgcgggccgg cgtcgccgat
      601 catccgcagc tcgcgggtgt cgagcagcgc gaccttgacc gtccgccggt acagcggcgc
      661 gatgcacacg atgtcgccgg ccgccgcccc gctgggttcg gcctccgcgg cgcggcctac
      721 caggacgtag agctccgcac tcagcgtggc gtggtaggcc tgccaccacg ggatcagcca
      781 gtccgggcca cggaacagcc cactgccgcc gacactggcg agcgcacgcc actctgcctc
      841 gatcgctcgg agcgccgcca tgtcgcggac gacggaaaca tcgactgcca ttcgcatcgc
      901 gacgttatca cgccgtgcca ggcgcggcgc gttgccatcc gcaacgcgct cgacaacgcc
      961 gcccgggcca tgtgggctca ctccatcggt accagactcc atggcgccag ccgatgcacg
     1021 agcgatgcct caatcgacac gccgcatcac gcgtgcatag aacccgtccg ttccgtggcg
     1081 gtgcggcgtc atggtgaatg cgtctccatc gccgagctcc cgcgcccggt cgcccaggat
     1141 ctcacggacc ggcacggtgg cgaggcccgg cccgaccacc gcggccacga cgtcggcgtt
     1201 ctcgatcgcg agcagcgtgc aggtcgcata gaccagccgg cccccggggg cgagcagggt
     1261 gcgcgcaccg ccgaggatct cctgctggcg cgcggcgaac tccgcgagat cggcttcgcg
     1321 gagccgccag cgcgcctcgg ggttgcggcg cagcgctccg atccccgagc acggcgcgtc
     1381 gaccagcacg acgtcggcct tgccgcgcag cgcgtcgagc tgcgccggcc agcgcccgcc
     1441 gtcgaggtgc accgtctggg cgttcgatac ccgggcgcgc cgggcgcgcc ggcgcagctc
     1501 gtccagcttg cgggcgtcga tgtcggcgga caggatccgc ccgcggttgc cgagccgggc
     1561 ggcgatcgcg agcgtcttgc cgccggcgcc cgcgcacaga tcgatgatga gccgcgcgtc
     1621 gcggcgcacg gaggggtgaa tcgcccggct ttgccgggcg agcccccaga gggggggtat
     1681 gggaaagccc ccctcttgat cgacgagccc gggcgcgccg gccgtgccgc gagcgcaggc
     1741 cgcggcaaga tccgcgagca gctggctgcc ctcgtcctgg gcctccatcg cgccgcgggt
     1801 gaacgccgcg agggcgaaca ggttggtgcg cgactcgagg tgcagcgcgg tatcgcacca
     1861 ccgccccggg agggtcgcga tccggtcgcg cgcgagctcg gccgccagtg cggtgcggtc
     1921 gccgatcagg aggttggcgc gcacggtcat cggcgcgcgc cggttgagcg cgagcgcgag
     1981 cgcttccgcc tcatcgcccc agtcggcggc gagccgcgcg gccagccagt cgggcagcga
     2041 ggccgcgagc gcgatccgcg gcacgagctt gcgctcgcgc gcgatcacct gatcgatctc
     2101 ggcgaccgcg ttccagtcga gctcgccgca cgtggcggcg gcgcgcgccg ggtcgatcag
     2161 gccgtccagc acgaacagcg cgagcagccg ctcgagatcg cgcggcgccc gctcggtcct
     2221 gcgcccgcgg gcgagcccgg cgtcgatccg gcgcaggtgg cgcacgagcc cgtacagcgt
     2281 ctcgctgacg aaccggcgct cgtgtgatcc gatccacgac tccttgcgga acgcggtggc
     2341 cagccggtcg gtcacgaagc cccagtccat gcgggtgcgc tgccagagct ccagcaccag
     2401 cgcgcacagc cggccaccgg cgacgacgtg cgccgccagc ttcctgtctg cgatgcgatc
     2461 gagatccggc aagacttgcg tcatacaccc gcaacgcggt gcggcgagcg ggttcgagcg
     2521 aaatcaccgc gatcacattg aaaacctgac ctccggagcg gtaagccgtg gctgatgagc
     2581 accgagacca agccgctacc gaccgtcaaa gaaggcggcg taaccagcct gaaggaacgc
     2641 ggaaatctgt tcgagatcgc gacgcgccag atcgacaagg cgatgaagct gatcaacatc
     2701 gatcccgata tcgcgcagat cctcgcgcag cccaagaacg agctgatcgt caacttcccg
     2761 gtccgcatgg acaacggcaa gttccagatg ttcaagggct accgggtcca gcacaacaac
     2821 atcctgggcc cgtacaaggg cggcatgcgc taccaccacg aggccaacct cgacgagatg
     2881 aaggcgctcg cgtcgtggat gacctacaag agcgcgctgc acgagatccc gttcggcggc
     2941 ggcaagggcg gcatcaaggt cgacccccgc cagcacagca aggccgagct cgagcgcatc
     3001 acccggcggt tcaccgccgc gctcggctcc aacatcggcc ccgagtacga catcccggcg
     3061 cccgacgtga acaccaacag ccagaccatg gtctggatga tggacacgta catgaacatc
     3121 gtcggggccg gcgatcgcaa caccgtgcgc ggcgtggtta ccggcaagag cgtgtcgtcg
     3181 ggcggcagcc acggccgcgc cgaggccacc ggccagggcg tcgtccactg catcaccgag
     3241 tgggcccggg acaagaactt caacctcgac ggctgcctgg tcatcatcca ggggttcggc
     3301 aacgtcggca gctacgccgc gcgcctcttg tcgcagaagg gcgcggtgat cgtcggggtc
     3361 ggcgacgccg gcggctacat cgccaacccc gagggcatca acccgcacaa gctcggcgag
     3421 cacgtccaca agaccggcac ggtcgccggc tacaagggcg cccagaagat cgagcgcgcc
     3481 gagttcttcg cgctcgacgc ccacatcttc gtccccgccg cgctcgagct cgagctcggc
     3541 gtcgccgagg ccaaggcgct caaggtcaag ctggtggtcg agggcgccaa cggcccgacc
     3601 taccccgagg ccgaggatgt cctgctcggc aagggcatcg acatcatccc cgacatcctg
     3661 tgcaactcgg gcggcgtgat cgtctcgtac tacgagtggc tgcagaacaa gcgcgccgag
     3721 cagtgggacg tcgaggacgt gctcgaccgg ctggagcgcc ggatgaagca gacctgggcc
     3781 aacgtccgcc actacgtcgc ggtcaacaag accgactggc gcaccgcgag ctacgcgatc
     3841 ggcctggagc gcctgcagca ctgctaccgc gagcgcggca tcttcccctg agctataacc
     3901 gcgcggtgcg cctcgtgatc gccgccgccg ccgcgctggt cctgggatgc ggtcgtcccg
     3961 ccgaccgccc tcccgttgtc ccggcgcagc cggcgtcccc tgggcatccc ggcgggaatg
     4021 ccgggcactc caccccggcg ccggtgacgc cgcccgggcc cggtgcggcc gcgc
//
LOCUS       DASVOR010001780         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_486121,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001780 DASVOR010000000
VERSION     DASVOR010001780.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486121"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="VF516_33100"
     CDS             <1..1228
                     /locus_tag="VF516_33100"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8112631.1"
                     /translation="PAAVSERVGLAPPAIEALARALGRARAPFLRIGGGLTRYGNGAM
                     TVRSIVALAAVLGVYDRPGGGCLHSTGSAYAFDMAPVFRSDLLARPTRTINMNQLGRA
                     LAELRDPPVRAMYVWCANPAAVAPDQNAVLRGLTRDDLFLVVHDRFLTDTARCADVVL
                     PATTSLEHADLYRAYGHYAVQRVQPAIPPLGEARPNWEVFRALAAGMGYDEPIFRMTA
                     DEVIDRLIERVDAAWLGDDDRAKLATGSAVMLVPPAGPRWHTPSGKIELHNPRQAEPL
                     PRDLPSHEDGGALPLRLVTAPALQTLNSSFQERPELRARAGGMVLQLGPSEAAARGLV
                     DGQRVIAANERGEAAFVLRISGGVPPGIAVAEGVFWLAHVPGQRNVNALTSQRLTDQG
                     AGSTFYDNRIDVRAAD"
     gene            1286..>2452
                     /locus_tag="VF516_33105"
     CDS             1286..>2452
                     /locus_tag="VF516_33105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8112632.1"
                     /translation="MAIPKQLSAAIDDAVSADAHDLLALSASLHANPELRFQEVKAAR
                     WISELVASRHIAVERDLGGLPTALRARAGSPGGARVAIVAEYDALPEIGHACGHNLIA
                     AGAVGAFLAAARVVASTGGEVVLLGTPAEEGGGGKIKLLEAGVFDGLDAAMMFHPADR
                     DLLAHIALASLWLTFEYTGVPSHAAAAPHIGASALTACMDTFRLIDGQRVHFKDGVRV
                     HGYITNGGQAVNIIPERAACEFSVRARDVEELERVRAIVERCARGAAMASGVELALSM
                     RPGYKDMRNNLTLARVFGEHLAAGGRRATEADPRLGAGSTDMGDVSHAVPSIHPYLAI
                     VGEGEAMCHEHRFAAAAGSDRGTDTALEAARARAKTAIELLTSPGLREAVRAEWS"
BASE COUNT          308 a          894 c          931 g          319 t
ORIGIN      
        1 gccggcggcg gtcagcgagc gggtcgggct ggcgcccccg gcgatcgagg cgctggccag
       61 agcgctcggc cgggcgcgcg caccgttcct ccggatcggc ggcgggctga cccggtacgg
      121 caacggcgcg atgaccgtgc gatcgatcgt cgcgctggcg gcggtgctcg gcgtgtacga
      181 tcgccccggc ggcggctgcc tgcactcgac cggctcggcc tatgcgttcg acatggcgcc
      241 ggtgttccgc agcgatctgc tggcgcgccc gacccggacg atcaacatga accagctggg
      301 gcgcgcgctc gccgagctcc gcgatccgcc ggtccgcgcg atgtacgtgt ggtgcgccaa
      361 ccccgcggcg gtcgcgccgg accagaacgc ggtgctgcgc gggctgaccc gcgacgacct
      421 gttcctcgtg gttcacgacc ggttcctgac cgacacggcg cgctgcgccg acgtggtgct
      481 gcccgcgacg acgtcgctgg agcacgccga tctgtatcgc gcgtatggcc actacgcggt
      541 ccagcgggtg cagccggcga tcccgccgct cggcgaggcg cggccgaact gggaggtgtt
      601 ccgcgcgctg gcggccggca tgggctacga cgagccgatc ttccggatga ccgccgacga
      661 ggtgatcgat cggctgatcg agcgcgtcga cgcggcctgg ctcggcgacg acgaccgcgc
      721 gaagctggcg accggcagcg cggtgatgct ggtcccgccg gccgggccgc gctggcacac
      781 gccgtcgggc aagatcgagc tccacaaccc acggcaggcc gagccgctgc cgcgcgacct
      841 gccgtcgcac gaggacggcg gcgcgctgcc gctgcgcctg gtcaccgcgc cggcgctgca
      901 gacgctgaac tcgtcgttcc aggagcggcc cgagctgcgc gcccgcgccg gtgggatggt
      961 gctccagctc gggccgtcgg aggcggccgc gcgcgggctg gtcgatggcc agcgcgtgat
     1021 cgcggccaac gagcgcggcg aggcggcgtt cgtgctgcgg atctcgggcg gtgtgccgcc
     1081 gggcatcgcg gtggccgaag gcgtgttctg gctggcccat gtccccggcc agcgcaacgt
     1141 caacgcgctg acctcgcagc ggctcaccga tcagggcgcc ggctcgacgt tctacgacaa
     1201 ccggatcgac gtccgcgccg cggactgagc tggatccgca ccggtactat tgacgcccgc
     1261 ggcgcgcccg gccgatcgtc ccggcatggc gatccccaag cagctcagcg ctgcgatcga
     1321 tgatgccgtg tccgccgatg cccacgatct gctcgcgctg tcggcgagcc tccacgccaa
     1381 ccccgagctc cggttccagg aggtcaaggc ggcccggtgg atctccgagc tggtcgcgtc
     1441 gcgccacatc gcggtcgagc gcgacctcgg cggcctgccg acggcgctgc gcgcgcgcgc
     1501 cggttccccg gggggcgccc gggtggcgat cgtcgccgag tacgacgcgc tgcccgagat
     1561 cggccacgcg tgcggccaca acctgatcgc ggccggcgcg gtcggcgcgt tcctcgccgc
     1621 ggcgcgggtg gtcgcgagca ccggcggcga ggtcgtgctg ctcggcacgc cggcggagga
     1681 aggcggcggc ggcaagatca agctgctcga ggccggcgtg ttcgacggcc tcgatgcggc
     1741 gatgatgttc cacccggccg atcgcgacct gctggcgcac atcgcgctcg ccagcctgtg
     1801 gctgacgttc gagtacaccg gcgtgccatc gcatgccgcg gccgcgccgc acatcggcgc
     1861 cagcgcgctc accgcgtgca tggacacgtt ccggctgatc gacggccagc gcgtccactt
     1921 caaggacggc gtgcgcgtcc acggctacat caccaacggc ggccaggcgg tcaacatcat
     1981 ccccgagcgc gcggcgtgcg agttcagcgt gcgggcgcgc gacgtcgagg agctcgagcg
     2041 ggtgcgggcg atcgtcgagc gctgcgcgcg cggcgcggcg atggcgagcg gcgtcgagct
     2101 cgcgctctcc atgcggcccg gctacaagga catgcgcaac aacctgacgc tggcgcgggt
     2161 gttcggcgag cacctcgccg ccggcggccg gcgggccacc gaggccgatc cgcggctcgg
     2221 cgcgggcagc accgacatgg gcgacgtttc gcacgccgtg ccgtcgatcc atccctacct
     2281 ggcgatcgtc ggcgagggcg aggcgatgtg ccacgagcac cggttcgcgg ccgccgccgg
     2341 gagcgatcga ggcaccgaca ccgcgctcga ggccgcccgg gcgagggcca agacggcgat
     2401 cgagctcctg acctcgccgg ggctgcgcga ggcggtgcgc gccgagtggt cg
//
LOCUS       DASVOR010001781        10830 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_164246,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001781 DASVOR010000000
VERSION     DASVOR010001781.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164246"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VF516_33110"
     CDS             <1..468
                     /locus_tag="VF516_33110"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8112633.1"
                     /translation="EVSVMESMYSPMAMVRTGPSYTMTDGAGWMVMVGVSIPLWRGKL
                     RAGVNEARAMADMASADLGAMQRMIGGEATAARERVAAARSRYLALRDSIVPRARQAI
                     APNLTAYAANQVPLVSAIEAAQALWEAQRELAMARAELGLAWARLRRTSGETP"
     gene            465..1967
                     /locus_tag="VF516_33115"
     CDS             465..1967
                     /locus_tag="VF516_33115"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8112634.1"
                     /translation="MTNEISTSTTTPSATPSEFGSAILQHQRKRRGPVLAVALVAGVA
                     GVLAGMQLRGSTGRSTATPTASGTAGMAGMPGMSHEGPPAPPGAGSQAVYISPARQQL
                     IGVRSAPIGRQRIEGAIRTVGMLAYDETRSAQIHTRVAGWVEQLYIDYVGKPVRRGQP
                     LFAVYSPDLATAQADYLVALRARQKIADNANPDLRAGADALVAASRDRMRRWNVSDAQ
                     IAELEKAGTPTRTVTIASPFDGVVLEKEAFAGQYVTPEMTSFKLADLSSIWAVGQVFE
                     YEAARLHVGDKVEVEFPYGQASKREATIDFIYPDVDPQTRRVRFRISLDNRDRKLKPD
                     TYVNLIWHGDATDRLVIPKEAVIDTGPRKYALLALANGYFAPRDVQVGPAVGDFYPVI
                     SGVAESDRVVTSAQFLVDSETNLMAAMQAMSMSMPGMDMGGMDMSKPGAMDPGQHEQP
                     GSASAGSAAMPGMDMPGMDMSQPGAKDHAHHVHPGSATGDHTTMPGMPGM"
     gene            1975..5145
                     /locus_tag="VF516_33120"
     CDS             1975..5145
                     /locus_tag="VF516_33120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEX8112635.1"
                     /translation="MINRIIQFSARNAFLVVLLIVAILGSGYWALRKTPIDAIPDLSD
                     VQVIIMTDWEGRSPTLVEDQVTYPIVTSLISAPRVKVVRGFSYFDVSFVYVLFEDGTD
                     PYWARTRVLEYLSALQGRLPEGVKSQLGPDASGVGWGFEYALTDKTGKHDLAQLRTLN
                     DWYVQYWLRSVPGVAEVAPYGGFVKQYQIEIDPNALAAFNIPLDMVVAAVRKSNNDVG
                     GRVVEWTGREYMVRGQGYVTGTADLEQVALGARPDGTPILVRDVARVHSGPEIRRGVA
                     DLNGEGDAAAGIVVIRQGVDTYGVIQNVKKVVKDKIQPALPEGVQFVTTYDRSDLIER
                     AIDTLKDKLVEEILIVSLVCIVFLWHLRSALVAILTLPLSILLSFVAMRALGIGANLM
                     SLGGIAIAIGAMIDAAIIMIENAHKQIEHDTSGRPRREILVEAAQQVGRPLFYSLLII
                     TVSFLPIFSLEAQEGRLFHPLAFTKTFAMGFAALTSVMIVPFLMIAFIRGKIPREERN
                     PVNRLLMRAYHPLVRFVLRHRVATLVAAAALLAGTVPVYLKLGSEFMPPLYEGTILYM
                     PTTLPGASIETARQALQLSDKLIMTVPEVASVSGKAGRALSATDPAPPEMFETVINLK
                     PESEWRPGMTQEKLVAELDRVVRVPGLANAWTMPIKARIDMLSTGIRTAVGIKVFGPE
                     LAKINDIGAQIEAALRPVVGTRNVFAERVTGGYYLDFKIKRDQIARYNLNVADVEMAI
                     ESAIGGANVTTTIEGRERYPVNIRYLRDYRTDIDAIRRTLIATPSGAQIPLEQVADIG
                     VSTGPTVLRTEQAQLLGYVYIDVGNRDLGSYVADAKAAVASAVKLPPGYTLEWSGQYE
                     YMQRASERLQIIIPITLGLVIVLLYLNTRRATKVGIVLLAVPFSLVGAFWLLWLLGYN
                     LSVAVWVGIIALAGVDAETGVVMLLYLDQSYDRFKTKGRMRNRQDLQEAVEDGAVKRI
                     RPKMMTVMAILMGLLPIMWSHGAGADVMKRIATPMVGGVITSFVLELLIYPVIFTVWK
                     GWREVKAGANGDGAAVTATARIRPSR"
     gene            complement(5250..5813)
                     /locus_tag="VF516_33125"
     CDS             complement(5250..5813)
                     /locus_tag="VF516_33125"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HEX8112636.1"
                     /translation="MTRRFLERRSERRYDAAPLPLDRLGELLGHLRAVSIDGKLKYGY
                     GSAGSSYPVQTYLYVKPGRVDQLAPGTWYYHPVEHRLLSIRPEVRIESKVHYFENRSI
                     FESSAFSIFFIGRMDAIAPLYGDKSRDFGLIEAGLMTQILELAATSAGIGLCQIGDMQ
                     FERVRDMFLLAPNDILLHSLLGGPLAG"
     gene            5724..6272
                     /locus_tag="VF516_33130"
     CDS             5724..6272
                     /locus_tag="VF516_33130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112637.1"
                     /translation="MPEKLAEPVERERRCIVPALAAAFEEPPRQLLFVFLLPKLHDAG
                     IWLCCSQAMPRGLEELALSRVEDHLDARPGGRIPPRGIRCHRSVRQGRRSGFCRHGRW
                     IYKKCTMRCGVLNTTPWRAVPAVSRDATMGSCRKMRPSHGKARREIFAARAPFPSIAQ
                     SLREGMPPDPAYAVLGSLADTV"
     gene            6289..6843
                     /locus_tag="VF516_33135"
     CDS             6289..6843
                     /locus_tag="VF516_33135"
                     /inference="COORDINATES: protein motif:HMM:NF027363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chalcone isomerase family protein"
                     /protein_id="HEX8112638.1"
                     /translation="MRRVLVVMFLAFFAGTAAARELAGVTMPETVTVDGKPLVLNGMG
                     IRKATIFNIKVYVVGLYLESKSRSAADILRSQQVKRFDVVLRRDVDRNDIIDAWRKGL
                     KRNSADMAKLKSEFDQFAGWMSDLAEGDTLTFLYVPERGVTVFVKGKLKGTIAGSDFA
                     TALFAMWLGANPVDDGLKDRLLGI"
     gene            7162..>10830
                     /locus_tag="VF516_33140"
     CDS             7162..>10830
                     /locus_tag="VF516_33140"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8112639.1"
                     /translation="MLCQPQLRAGDFCVIASAGDIEAWLVRHLAERLDVSCDAIDPCE
                     PFASHGLTSREAVVLSGELEEWLERTLSPTLVWEHPTIRDLARFLAGVAPGAATTPDP
                     APRHGGEDHIAIVGLGCRFPGAPDPDAFWNLLWSGDDAIREVPADRWDVGAFYDPDPA
                     APGTTTTRCGGFLTRVDEFDPSFFCISPREASHMDPQQRLLLEVAWEALENAGIAPQS
                     LYGTETGVFVGISTSDYAGLQLSDPARVGAHDGTGSAHSIAANRLSYLLDLRGPSMAV
                     DTACSSSLVAVHLACQSLRIGECRVALAAGANVMLTPQLTIVFSKAGMMAADGRCKTF
                     DAAADGYVRGEGCGVVILKRLADALADGDQVLAVVRGSAVNQDGLSNGLTAPNGLAQE
                     AVIRAALARAGVSPHDISYGEAHGTGTPLGDPIEVAALGAVLMPERPRDRPCALASVK
                     TNIGHLEAAAGIAGLIKTVLALVHREIPPHLHLHTVNPKIRLEGTSFVIPRTLLPWSS
                     PEGPRRAGVSAFGFGGTNVHVILEEAPGAAPRAVDAVERGPEVLLLSAQSDAALRALA
                     ERYAERLETVPATEIADACATARRGRYPFSHRLAVIGDSGSQLRSRLLAFTTSDDLPQ
                     VVCGHVRRKTPPKVAFLFSGQGSQYAGMGRGLYETEPVFRRALDRCEELLRPHLGVPL
                     FSVLWPAAGAPPLLDETGYTQPALFALQYALTELWRSLGVVPAAVVGHSVGEFAAACA
                     AGVLDVDAAVALVAARGRLMQALPQDGGMAAVFASEDEVAAVLAAAPGRLSIAAVNGP
                     ADTVVSGPRLELDVLLQVLAARGVKARRLVVSHAFHSCLMDPMLDAFTAEVERVELTA
                     PRIPLISNVTGEVAPAEVVARADYWRRHVRAPVRFAAGVRALQALGCEAFVEIGPGAT
                     LIGLGQRCLPGDAELWVASMRKGPCERTQLLTAVGGLFTSGIPLDWAGLDAGRPRRSL
                     ALPTYPFERQRCWLETGGSVAPPRSQVEGAVHGGQSIGDTLYQVAWGAVDRPAPDPAK
                     RPGHWVVLADDGSVGKALRIELEARGATCEVPDAAASLDDIARAAAGARGIVYLRALD
                     ARIPEDPERVNARALARAQHLSCAGALQLVHALIAAGTACRTWLVTCRSQAVRPEDAV
                     EIGQAPLWGFGRTLALEHPELWGGLVDLDDEPSRALAAALADELLGEAGEDQVAYRKG
                     QRHVARLERRPDASAHTGS"
BASE COUNT         1690 a         3585 c         3795 g         1760 t
ORIGIN      
        1 gaggtctcgg tgatggagtc gatgtactcg ccgatggcga tggtgcgcac cgggccgtcg
       61 tacacgatga cggacggcgc cgggtggatg gtgatggtcg gcgtcagcat tccgctgtgg
      121 cgcggcaagc tgcgcgcggg cgtgaacgag gcgcgcgcga tggccgacat ggccagcgcc
      181 gatctgggtg cgatgcagcg gatgatcggc ggcgaggcga ccgcggcgcg tgagcgagtc
      241 gccgcggcgc ggtcgcgcta cctcgcgctg cgcgacagca tcgttccgcg agcgagacag
      301 gcgatcgccc ccaacctcac cgcctacgcc gccaaccagg ttcccctggt gagcgcgatc
      361 gaagccgcac aggccctgtg ggaagcgcag cgcgagctgg ccatggcgcg cgccgagctc
      421 ggcctcgcct gggcacgact gcgccggacg agcggagaga caccatgacg aacgagatct
      481 cgacatcgac gaccacaccc tccgccacgc cgtcggagtt cggcagcgcc atcctgcagc
      541 accagcgcaa gcgccgcggg ccggtcctcg cggtcgcgct cgtcgccggc gtcgcgggtg
      601 tgctggcggg catgcagctc cgcggatcga ccggccggtc cacggctacg ccgacggcga
      661 gcgggacggc tggcatggca ggcatgcccg gcatgtctca cgaggggccg cccgcgccgc
      721 caggggcagg ctcgcaagcg gtgtacatct cgccggcgcg gcagcagctc atcggagtgc
      781 gcagcgcgcc gatcggccgc cagaggatcg agggagcgat ccgcaccgtc gggatgcttg
      841 cgtacgacga gacgcgcagc gcgcagatcc atacccgcgt ggccggctgg gtcgagcagc
      901 tctacatcga ctacgtcggc aagccggtgc ggcgtggcca gccgctgttc gcggtgtaca
      961 gcccggatct ggcgaccgcg caagcagact acctggtcgc gctgcgcgcg cggcagaaga
     1021 tcgccgacaa cgcgaacccc gacctccgcg ccggtgccga tgcgctggtg gcggcatcgc
     1081 gggaccgcat gcggcgctgg aacgtcagcg acgctcagat cgccgagctc gagaaggcgg
     1141 gcactccgac ccgcacggtg acgatcgcgt cgccgttcga cggcgtcgtg ctcgagaagg
     1201 aagcgttcgc agggcagtac gtcactccgg agatgacgtc gttcaagctc gccgacctga
     1261 gctcgatctg ggcagtggga caggtgttcg agtacgaggc cgctcggctc cacgtgggcg
     1321 acaaggtcga ggtcgagttc ccctacggcc aggcgagcaa gcgcgaggcg acgatcgact
     1381 tcatctaccc cgatgtcgat ccccagaccc ggcgcgtccg gtttcggatc tcgctggaca
     1441 accgcgatcg caagctcaag cccgacacgt acgtgaactt gatctggcat ggcgacgcga
     1501 ccgatcggct cgtgatcccg aaggaggcgg tgatcgatac cggtccgcgc aagtacgcgt
     1561 tgctcgcgct cgcgaacggc tattttgcgc cacgcgacgt gcaggtcgga cccgcggtcg
     1621 gcgacttcta tccggtgatc tccggagttg ccgagagcga tcgggtggtg acgtcggcgc
     1681 agttcctggt cgactccgag accaacctga tggcggcgat gcaggcgatg tcgatgtcga
     1741 tgcccggcat ggacatgggc ggcatggaca tgagcaagcc gggcgcgatg gaccctggcc
     1801 agcacgagca gccggggtcc gcgagcgccg gcagtgcagc gatgccaggg atggacatgc
     1861 cgggcatgga tatgagccag ccgggcgcga aggatcatgc gcatcacgtg caccccggct
     1921 cggcgaccgg cgaccacacg acgatgccgg gcatgccggg aatgtgagga cgccatgatc
     1981 aaccgcatca tccagttctc tgcgcgcaat gcgttcctgg tcgtgctgct gatcgtcgcg
     2041 atcctgggtt cgggatactg ggcgctgcgc aagacgccga tcgatgccat cccggatctg
     2101 tccgacgtgc aggtcatcat catgaccgac tgggagggcc gcagcccgac actcgtcgag
     2161 gaccaggtca cctatccgat cgtcacctcg ctgatctccg cgccgcgcgt caaggtggtg
     2221 cgcgggttct cgtacttcga cgtgtcgttc gtctacgtcc tcttcgagga tggcaccgat
     2281 ccatactggg cgcgcacccg cgtactggag tacctgagcg cgctgcaggg ccggctaccc
     2341 gagggcgtga agtcccagct cggccccgac gcgagcggtg tcggctgggg gttcgagtac
     2401 gcgctcaccg acaagaccgg caagcacgac ctcgcgcagc tgcgcacgct caacgactgg
     2461 tacgtgcagt actggctgcg cagcgtgccc ggcgtcgccg aggtcgcgcc gtatggcggc
     2521 ttcgtcaagc agtaccagat cgagatcgat ccgaacgcgc tcgccgcgtt caacatcccg
     2581 ctcgacatgg tcgtggcggc ggtgcgcaag agcaacaacg acgtcggcgg ccgcgtcgtc
     2641 gagtggaccg gccgagagta catggtgcgc ggccagggct acgtgaccgg gaccgccgat
     2701 ctggagcagg tggcgctcgg cgcgcggccc gatggcacgc cgatcctcgt gcgcgacgtc
     2761 gcgcgcgtcc acagcgggcc cgagatccgg cgcggcgtgg cagatctcaa cggcgagggg
     2821 gacgccgccg ccggcatcgt ggtgatccgc cagggcgtcg acacctacgg cgtgatccag
     2881 aacgtcaaga aggtcgtcaa ggacaagatc cagccggcgc tccccgaggg cgtgcagttc
     2941 gtgaccacct acgatcgctc cgacctgatc gagcgtgcga tcgacacgct gaaggacaag
     3001 ctggtcgagg agatcctgat cgtctcgctg gtctgcatcg tgttcctgtg gcacctgcgc
     3061 agcgcgctgg tcgcgatcct gaccctgccg ctgtcgatcc tcctgtcgtt cgtcgcgatg
     3121 cgggcgcttg gcatcggggc gaacctgatg agcctcggcg ggatcgcgat cgcgatcggc
     3181 gcgatgatcg acgccgcgat catcatgatc gagaatgcgc acaagcagat cgagcatgac
     3241 accagcggcc ggccgcggcg cgagatcctg gtcgaggcgg cgcagcaggt cgggcgcccg
     3301 ctgttctact cgctcctgat catcacggtg tcgttccttc cgatcttctc gctggaggcg
     3361 caggagggcc ggttgttcca tccgctcgcg ttcaccaaga cattcgcgat ggggttcgcg
     3421 gcgctgacct cggtcatgat cgtgccgttc ctgatgatcg cgttcatccg cggcaagatc
     3481 cccagggagg agcggaaccc tgtcaaccgg ttgctgatgc gggcctatca cccgctggtg
     3541 cgcttcgtgc tgcggcaccg ggtcgcgaca ctggtcgccg ccgccgcgct gctcgccggc
     3601 acggtcccgg tctacctgaa gctcggctcg gagttcatgc cgccgttgta cgaagggacg
     3661 atcctgtaca tgccgaccac cttgccaggc gcgtcgatcg agaccgcgcg gcaggcgttg
     3721 cagctgtcgg acaagctgat catgaccgta ccggaggtcg cctcggtgtc cggcaaggcg
     3781 ggccgcgcgc tgtcggccac ggatcctgcg ccccccgaga tgttcgagac cgtgatcaac
     3841 ttgaagcccg agtccgagtg gcggcccggc atgacgcagg agaagctcgt cgccgagctc
     3901 gaccgcgtgg tccgcgtgcc gggcctggcc aatgcctgga cgatgccgat caaggcgcgc
     3961 atcgacatgc tgtcgaccgg gatccggacc gccgtcggga tcaaggtgtt cgggccggag
     4021 ctcgcgaaga tcaacgacat cggggcgcag atcgaggccg cgctccgtcc ggtcgtgggg
     4081 acgcgcaacg tgtttgccga gcgggtcacc ggcggctact acctcgactt caagatcaag
     4141 cgcgaccaga tcgcgcgcta caacctcaac gtcgccgatg tcgagatggc gatcgaatct
     4201 gcgatcggcg gcgcgaacgt caccacgacc atcgagggcc gcgagcgcta cccggtcaac
     4261 atccgctacc tgcgcgacta ccgcacggac atcgacgcga tccgccgtac gctgatcgcc
     4321 acgcccagcg gcgcgcagat cccgctcgag caggtggccg acatcggagt gtccaccggt
     4381 cccacggtcc tgcgcaccga gcaggcccag ctcctggggt acgtctacat cgacgtcggc
     4441 aaccgcgacc tcggcagcta cgtcgcggac gccaaggccg ccgtcgccag cgccgtgaag
     4501 ctgccgccgg gatacacgct ggagtggagc ggtcagtatg agtacatgca gcgtgcgagc
     4561 gagcggctgc agatcatcat cccgatcacg ctcgggctgg tcatcgtgct gctctatctc
     4621 aacacccgtc gcgcgaccaa ggtcggcatc gtcctgctcg ccgtgccgtt ctcgctggtc
     4681 ggggcgttct ggttgctgtg gttgctcggc tacaacctca gcgtcgcggt gtgggtcggg
     4741 atcatcgcgc tcgccggcgt cgacgccgag accggcgtgg tgatgctgct ctacctcgac
     4801 cagtcctacg accgattcaa gaccaaaggt cgcatgcgca accgacagga cctccaggag
     4861 gccgtggagg acggtgcggt caagcgcatc cggccgaaga tgatgacggt gatggcgatc
     4921 ctcatgggcc tgttgccgat catgtggagc cacggcgcgg gtgccgacgt catgaagcgg
     4981 atcgccacgc cgatggtcgg cggggtcatc acgtccttcg tcctcgagct cctcatctat
     5041 cccgtgatct ttaccgtgtg gaaggggtgg cgcgaggtga aggccggcgc gaacggcgac
     5101 ggggccgcgg tcaccgcaac cgcgcgcatt cggcccagcc gttgagctga ggcctcggga
     5161 cctactcggt gtgatcgcgc cggtaacacg taaccgaacc ggtcatcgat tcgatgtgag
     5221 tacacctgtg acggcgagcc ggcccccgat cagcccgcga gcggaccgcc gagcaggctg
     5281 tgcaacagga tgtcgttcgg agcgagcagg aacatgtccc gcacccgctc gaactgcatg
     5341 tcgccgatct ggcacagccc gatacctgcg gatgtcgccg ctagctccag gatctgggtc
     5401 atgagcccgg cctcgatgag gccgaagtca cggctcttgt cgccgtagag cggcgcgatc
     5461 gcgtccatcc ggccgatgaa gaagatcgag aacgcggacg actcgaagat cgagcggttc
     5521 tcgaagtaat gaaccttgga ctcgatccgg acctcggggc gaatcgatag cagccggtgc
     5581 tcgacgggat gatagtacca ggtgccgggc gcgagttggt ccacgcgccc cggcttcacg
     5641 tacagatagg tctgtacggg gtacgagctg cccgccgagc cgtagccgta cttgagcttg
     5701 ccatcgatgc tcacggcccg gagatgcccg agaagctcgc cgagcctgtc gagcgggagc
     5761 ggcgctgcat cgtaccggcg ctcgctgcgg cgttcgagga acctccgcgt cagctgctct
     5821 tcgtcttcct gcttccgaag ctgcacgacg ccggcatctg gctctgctgc tcgcaagcca
     5881 tgccgcgcgg ccttgaagag ctcgcgctgt cgcgggtcga agatcatctc gatgcgcgtc
     5941 caggcgggcg gatcccgccg cggggcattc gctgccatcg ctccgtgcgg caaggtcgac
     6001 ggtcgggttt ctgtcgccat gggcgctgga tctacaagaa atgcaccatg cgctgcggcg
     6061 tcctgaacac gaccccatgg cgcgcagttc ctgccgtttc gagggacgcg acgatggggt
     6121 catgccgcaa gatgcgccca tcgcatggca aggcgaggcg cgaaatcttc gcagctcggg
     6181 cgccttttcc ctcgatagcg caatctctgc gcgaaggcat gccgcctgac ccggcatacg
     6241 ccgtgcttgg ttcgctcgcg gacacggtgt gatcgtttgg aggtggccat gcgacgcgtc
     6301 ctggttgtga tgttccttgc attcttcgcc ggcaccgcgg cggcccgtga gctcgccggt
     6361 gtcaccatgc ccgagaccgt cactgttgac gggaagcccc tggtcttgaa cgggatgggc
     6421 atccgcaagg cgacgatctt caacatcaag gtctacgtgg tggggctcta cctcgagagc
     6481 aagtcgcgca gcgcggcgga cattcttcgc tcccaacagg tgaagcgctt cgacgtcgtg
     6541 ttgcgacgtg acgtcgatcg caacgacatc atcgatgcgt ggcgcaaggg cctgaagaga
     6601 aacagcgccg acatggcaaa gctcaagtcc gagttcgacc agttcgccgg ctggatgtcc
     6661 gacctcgccg agggagatac gctcacgttc ctctacgtcc cggagcgcgg cgtcacggtg
     6721 ttcgtcaagg gaaagctgaa gggaaccatc gccggcagcg atttcgcgac ggcgctgttc
     6781 gcgatgtggc tcggagccaa cccggtggac gacgggctca aggacagact gctcgggatc
     6841 tgagtcacag atattggatg tggtgactct gtgatctgag agcgcactgg cagagtggat
     6901 gtttcctcag cctcgccatt gaagcgctcc cggtgagcgt gattcatggt ttgtaatacg
     6961 gcgcgaggcg cgcggttcca ggagttattc ggagaatcag ccgtcaatgg catcctgtct
     7021 gcaatggagg gtcggcatca tgaggcgaga agtcagggtg aatacaccgc cgcggcattt
     7081 gcttgttcat tggatgcgcc aacaagacaa caatgcgggt ttgcgcgtcc ttcactcgaa
     7141 gcggtcggtc gcgaagcgcg aatgctctgc cagccgcagc tccgagccgg agatttttgc
     7201 gtgatcgcct cggccggcga catcgaagca tggctcgttc gccatctcgc ggagcgtctc
     7261 gacgtctcat gtgacgcgat cgatccctgc gagcccttcg cgagccatgg attgacgtct
     7321 cgcgaagctg tcgtgctgtc gggcgagctg gaggagtggc tcgagcgaac gctgtcgccg
     7381 acgctggtct gggaacaccc gacgatccgg gatctcgccc ggttcctggc gggcgttgca
     7441 ccgggggcgg ccaccacacc cgatccggct ccacgccacg gcggcgagga ccacatcgcg
     7501 atcgtgggcc tggggtgtcg attcccgggc gcgccggatc cggatgcgtt ctggaacctc
     7561 ctctggtcgg gcgacgatgc gatccgggaa gtgcccgccg atcgctggga cgtcggtgcg
     7621 ttctacgatc cggatcccgc ggcgcctggt acgaccacga cccggtgcgg cggcttcctg
     7681 acccgggtcg atgaattcga tccgagcttc ttctgcatct cgccgcgcga ggcgagccac
     7741 atggatccgc aacagcggct gctgctggag gtggcctggg aggcactgga gaacgccggg
     7801 atcgcgccgc aatccctgta tggcacggag accggtgtat tcgtcggaat cagcaccagc
     7861 gactacgccg gattgcaact ctcggatcct gcgcgcgtgg gagcccatga tgggaccgga
     7921 agcgcgcaca gcatcgccgc gaaccggctg tcctatctgc tcgatctgcg cggccccagc
     7981 atggctgtgg acaccgcgtg ctcgtcgtcg ctcgtggcgg tgcacctcgc gtgtcagagc
     8041 ctgcgcatcg gcgagtgccg ggtcgccctg gcggccggcg ccaacgtgat gctcacgccg
     8101 cagctcacga tcgtgttctc caaggccggc atgatggctg cagacgggcg gtgcaagacc
     8161 ttcgacgccg cggccgatgg ctatgtgcgg ggcgagggct gcggcgtggt gatcctgaag
     8221 cggctcgccg atgcgctcgc cgacggcgac caggtgctcg cggtcgtgcg cggctcggcg
     8281 gtgaaccagg atgggctgag caacgggctc acggcgccga acgggctcgc ccaggaggcg
     8341 gtgatccggg ccgcgctcgc gcgcgctggc gtctcgccac acgacatcag ctacggcgag
     8401 gcgcatggca ccgggacgcc gcttggcgat ccgatcgagg tcgctgcgct cggtgcggtg
     8461 ctgatgccgg agcgcccgcg ggaccggccg tgcgcgctgg catcggtgaa gaccaacatc
     8521 ggtcacctcg aagccgcggc cgggatcgcc ggactcatca agacggtgct cgcgctcgtg
     8581 catcgcgaga ttccacccca cctgcacctc cataccgtca atccgaagat ccgcctcgaa
     8641 ggtacgagct tcgtcatccc gcgcacgctc ctgccctggt cgtcgccgga aggtccgcgt
     8701 cgggctggcg tgagtgcgtt cgggttcggc ggcacgaacg tccacgtgat cctggaggag
     8761 gctcctgggg ccgccccccg cgcggtcgac gccgtggagc gcggtccgga ggttcttctg
     8821 ctctcggcac agagcgatgc ggccctgcgg gcccttgcgg agcgctatgc ggagcggctc
     8881 gagaccgtgc ccgccaccga gatcgccgat gcgtgcgcca cggcgcgccg cggtcgctat
     8941 ccgttcagcc accggctcgc ggtgatcggc gattccggat cgcagctccg gtcgcgactc
     9001 ctggcattca ccacgagcga cgatcttccg caggtcgtct gcgggcacgt gcggcgcaag
     9061 accccgccca aggtggcctt cctgttcagc ggtcagggat cgcagtacgc gggcatggga
     9121 agagggctgt acgagaccga gccggtgttc cgccgggcgc tggatcgatg tgaggagctg
     9181 ctccggccgc acctcggtgt tccgctgttc tcggtgctgt ggcccgccgc gggcgcgccg
     9241 ccgctgctcg atgagaccgg gtacacgcaa cccgcgctgt tcgccctcca gtacgcgctg
     9301 accgagctgt ggcgctcgct cggcgtcgtc cctgccgcgg tggtcggaca cagcgtcggc
     9361 gagttcgcgg ccgcgtgcgc cgcgggtgtg ctcgacgtcg acgccgcggt cgcgctcgtc
     9421 gctgcgcgcg gccgcctgat gcaggcgctg ccgcaggacg gcggcatggc tgccgtgttc
     9481 gcctccgagg acgaggtcgc ggcggtcctc gcggcggcgc cgggacggct gtcgatcgcg
     9541 gccgtgaatg gacctgccga caccgtggtg tcggggccca ggctcgagct cgacgttctc
     9601 ctgcaggtgc tcgcggcgcg cggagtgaaa gcccggcgcc tggtcgtgtc gcatgccttc
     9661 cactcctgcc tgatggaccc gatgctcgac gcgttcaccg ccgaggtcga acgcgtcgag
     9721 ctcaccgccc cacgcatccc gctgatctcg aacgtcaccg gcgaggtcgc cccggccgag
     9781 gtggtcgcgc gcgcagacta ctggcgccgg cacgtccgtg ctccggtgcg attcgcggcc
     9841 ggagtgcggg cattgcaagc cctggggtgc gaagccttcg tcgagatcgg gcctggcgcg
     9901 acgctgatcg gcctcgggca gcgctgcctc cccggcgacg cggagctctg ggtcgcgtcg
     9961 atgcgaaagg ggccctgcga gcgcacccag ctgttgacgg ccgtcggggg gctgttcacc
    10021 tctggcatcc cgctcgactg ggcggggctc gacgccggac gcccgcggcg ctcgctggcg
    10081 ctgccgacct atccgttcga gcgccagcgc tgctggctgg agaccggcgg ttcggttgcg
    10141 ccgccgcgca gccaggtcga gggtgcggtg cacggtggcc agagcatcgg cgacacgctt
    10201 taccaggtgg cctggggcgc ggtggaccgg ccggctccgg atccggcgaa gcgacctgga
    10261 cactgggtcg tcctcgcgga cgacggcagc gtcgggaagg cactccgcat cgagctggag
    10321 gcgcgcggtg cgacctgcga ggttccagac gctgcggcat ccctcgacga cattgcgcgt
    10381 gcggctgccg gcgcgcgcgg gatcgtctac ctccgcgcgc tcgacgcgcg aatccccgag
    10441 gatcccgaac gcgtgaacgc gcgagcgctg gcccgggcgc agcacctgag ctgcgccggc
    10501 gcgctgcagc tggtgcacgc gctgatcgcc gccgggacgg cgtgtcgaac ctggctggtc
    10561 acctgccggt cccaggcggt gcggcccgag gatgcggtcg agatcggcca ggcgccgctg
    10621 tggggcttcg gccgcacgct ggcgctggag cacccggagc tatggggcgg gctggtcgac
    10681 ctcgacgacg aaccgtcgcg cgcgctggcc gccgcgctcg ccgatgagct gctcggcgaa
    10741 gccggcgagg accaggtcgc gtaccggaag ggccagcgtc acgtggcacg gctcgagcgc
    10801 cgcccggatg cttcggcgca caccgggtcg
//
LOCUS       DASVOR010001782         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_47909,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001782 DASVOR010000000
VERSION     DASVOR010001782.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47909"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2132
                     /locus_tag="VF516_33145"
     CDS             <1..>2132
                     /locus_tag="VF516_33145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEX8112640.1"
                     /translation="RERRLRKRAAAAGLPIVAATEVLYHSRARRPLQDVLTCIRHGVT
                     LVTAGRRIRGNDEHDLCAPHRFTRLFADAPEAVDRTREIAARCRFGLGELRYRYPSER
                     LPSGLTSAAYLRDRTYDGAAERFGGAIPPDVRSQLDAELALIEELDYPGYFLTMYEIV
                     SYCRRRGILCQGRGSAANSAVCFCLGITAIDPVRMGLLFERFLSRERAEPPDIDLDIE
                     HERREEVIQHAYTVYGRDHAAMVCNVIRYRPRSAVRDVGKVLGISETALDRAAKQLSM
                     YGAVEDAALARAGLGEHGESVFGHLVKLSQEILEFPRHLSIHPGGFLLGHEPVHDIVP
                     IENAAMPGRTVIQWDKDDLEDLGLFKVDLLGLGALHQLHLAFDLLRAHRGIELTMATI
                     PAEDEATYDMICTADTVGTFQIESRAQMSMLPRLRPREFYDLVVEVSLVRPGPISGGM
                     VHPYLRRRHGTEPIDYPHPCLEQVLKKTLGVPLFQEQVMKIAIVAADYTPGEADQLRR
                     DMAAWRRSGRIEKHHERLVSRMEHKGIAREFAERVFEQIRGFGEYGFPESHAASFALI
                     AYATSWLRKHYLAEFTCALLNAQPMGFYSPATIVGDAQRHGLEVRPIDVTRSAWDCTL
                     EPTEGGPGGGGGGHGFAVRMGLRWIKGLAFADGQRIVAARLDCPFASVEDFVRRSRVP
                     ARSHSALAEAGALAALAGPSRRDALWQVT"
BASE COUNT          287 a          801 c          750 g          294 t
ORIGIN      
        1 cgcgagcgcc ggctgcgcaa gcgggccgcc gcggccggcc tgccgatcgt cgccgcgacc
       61 gaggtgctct accactcgcg ggcgcgccgc ccgctgcagg acgtcctgac ctgcatccgc
      121 cacggcgtca cgctggtcac cgcgggccgc cggatccgcg gcaacgacga gcacgacctg
      181 tgcgcgccgc accgcttcac ccggctgttc gccgacgctc ccgaggcggt cgaccgcacc
      241 cgcgagatcg ccgcgcgctg ccggttcggg ctcggcgagc tgcgctaccg ctatccgtcc
      301 gagcgcctgc cgagcgggct gacctcggcg gcgtacctgc gcgatcggac ctacgacggc
      361 gccgccgagc ggttcggggg cgcgatcccg cccgacgtgc gcagccagct cgacgccgag
      421 ctcgcgctga tcgaggagct cgactacccc ggctacttcc tcacgatgta cgagatcgtg
      481 tcgtactgcc ggcgccgcgg tatcctgtgc cagggccgcg gctcggcggc gaactccgcg
      541 gtgtgcttct gcctcggcat caccgcgatc gacccggtgc gcatgggcct tctgttcgag
      601 cgcttcctgt cgcgcgagcg cgccgagccg cccgacatcg acctcgacat cgagcacgag
      661 cgccgcgagg aggtgatcca gcacgcctac accgtgtacg gccgcgatca cgccgcgatg
      721 gtgtgcaacg tgatccgcta ccgcccgcgc tcggcggtgc gcgacgtcgg caaggtgctc
      781 gggatctccg agaccgcgct cgaccgcgcc gccaagcagc tgtcgatgta cggtgcggtc
      841 gaggacgccg ccctggcgcg cgccgggctg ggcgagcacg gcgagagcgt gttcggccac
      901 ctggtcaagc tgtcccagga gatcctcgag ttcccgcgcc acctgtcgat ccaccccggc
      961 gggttcctgc tcggccacga gccggtccac gacatcgtgc cgatcgagaa cgcggcgatg
     1021 ccgggccgca ccgtgatcca gtgggacaag gacgacctcg aggacctcgg gctgttcaag
     1081 gtcgacctgc tcgggctcgg cgcgctgcac cagctccacc tggcgttcga cctgctgcgt
     1141 gcgcaccgcg ggatcgagct gacgatggcg acgatcccgg ccgaggacga ggcgacctac
     1201 gacatgatct gcaccgccga caccgtcggc acgttccaga tcgagagccg ggcgcagatg
     1261 tcgatgctgc cgcggctccg gccgcgcgag ttctacgacc tcgtcgtcga ggtcagcctg
     1321 gtccgcccgg ggccgatctc cggcggcatg gtccacccct acctgcggcg gcgccacggc
     1381 accgagccga tcgactaccc tcacccctgc ctggagcagg tgctgaagaa gacgctcggc
     1441 gtgccgctgt tccaggagca ggtcatgaag atcgcgatcg tcgcggccga ctacacgccg
     1501 ggcgaggccg accagctgcg ccgcgacatg gcggcgtggc gccgctcggg ccggatcgag
     1561 aagcaccacg agcggctggt cagccgcatg gagcacaagg gcatcgcccg cgagttcgcc
     1621 gagcgcgtgt tcgagcagat ccgcgggttc ggcgagtacg gcttccccga gtcgcacgcc
     1681 gcgagcttcg cgctgatcgc ctacgccacg tcgtggctgc gcaagcacta cctggccgag
     1741 ttcacctgcg cgctgctcaa cgcccagccg atggggttct actcgccggc gacgatcgtc
     1801 ggcgacgccc agcgccacgg cctggaggtc cggccgatcg acgtcacccg cagcgcctgg
     1861 gactgcacgc tggagccgac cgagggcggc ccgggcggcg gcggcggcgg ccacggcttc
     1921 gcggtgcgca tgggcctgcg ctggatcaag ggcctggcgt tcgccgacgg ccagcgcatc
     1981 gtcgcggcgc ggctcgattg cccgttcgcc tcggtcgagg acttcgtgcg ccggtcgcgc
     2041 gtcccggcgc gcagccacag cgcgctcgcc gaggccggcg cgctcgctgc gctcgccggg
     2101 cccagccgcc gggacgcgct gtggcaggtc ac
//
LOCUS       DASVOR010001783         2855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_47938,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001783 DASVOR010000000
VERSION     DASVOR010001783.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47938"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VF516_33150"
     CDS             <1..216
                     /locus_tag="VF516_33150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112641.1"
                     /translation="APPAEPSAPPADPGPRRYVRFDVPNPAVQVTQKADGTFDIRSTD
                     PSLAGSILTVTAISESGAEDKVLIRVP"
     gene            complement(302..568)
                     /locus_tag="VF516_33155"
     CDS             complement(302..568)
                     /locus_tag="VF516_33155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112642.1"
                     /translation="MKKLVVLTSLSAIITIASTISAADNPQDPPDANRFRCVMYNDDG
                     SCTSMPADCPVTGPAHPNNPSCPEGWTLILAQNDNCTQQLICVD"
     gene            complement(1223..1501)
                     /locus_tag="VF516_33160"
     CDS             complement(1223..1501)
                     /locus_tag="VF516_33160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112643.1"
                     /translation="MLGVQRSDARLDGAACDLAPPTLRLGDQQPEVVVDARSFAVAAP
                     GNGGAAPLRFVSGVSAFAAALEPTGCLASALYGARCRVARMYRAFSPK"
     gene            1621..1839
                     /locus_tag="VF516_33165"
     CDS             1621..1839
                     /locus_tag="VF516_33165"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112644.1"
                     /translation="MDALEVHAAHGYLLHELLSPLANQRGDAYGGSLANCLRLPLEVF
                     EAVRAVFPEDRPVGVRVSRPIPASPDRS"
     gene            2353..>2855
                     /locus_tag="VF516_33170"
     CDS             2353..>2855
                     /locus_tag="VF516_33170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112645.1"
                     /translation="MYQSYQLIGYQTPTTKLGPGDLVNLDAYADLIGDLKTNDGWLSD
                     IKNADMKKRLARLIGVVEQAHALQGQQVSNDQTTLKVFLVPEFYFRPTADQGVQSSYS
                     DAERDALVKVLDRYVKAERFKHWLFVFGTVIWQIQVSKLMANAQLPDGVKLNDDKAQA
                     YVIKNTTL"
BASE COUNT          506 a          981 c          922 g          446 t
ORIGIN      
        1 gccccgcccg ccgagccatc ggcgccgccg gccgacccgg ggccgcgccg ctacgtgcgg
       61 ttcgacgtcc ccaacccggc ggtccaggtg acccagaagg ccgacggcac gttcgacatc
      121 cgctcgaccg atcccagcct cgccgggtcg atcctgacgg tgaccgcgat cagcgagtcc
      181 ggcgcggagg acaaggtgct gatccgcgtc ccgtagcccg catcagtcac caactcgagc
      241 acgccattcg cgcgagcgtg cagatggcgt gagcggcagg tacgcccgct tcggctgcgc
      301 gtcagtcgac gcagatcaac tgctgagtgc agttgtcatt ttgagccagg atgagcgtcc
      361 agccctcggg gcaactggga ttgttgggat gggccggacc ggtgactgga cagtccgccg
      421 gcatggacgt gcagctgcca tcgtcattgt acatgacgca ccggaagcga ttcgcatccg
      481 gtgggtcttg cgggttgtcg gccgcggaga tggtggaggc aatggtgatg atcgcggaca
      541 gagaggtaag cacgactagc tttttcatat gagtacaatc ctttctttcg caatacggaa
      601 aatgacaacg gaagctttcc gagctctggc cggtgtcgcg gcgagcagcg aattatccgt
      661 acgagcaccg gcgagccagg atcgaggcgc gtcgtccctc gatgatcaga cgctgctggc
      721 cgtcgcgggc agcggtcgcg atcgccggat tcccgcgagc tcggataagc cgcggcggcg
      781 tcaaggacgt gctaaacgat ccgcatgcgc ccgccgagcc agatcgggag gaccagcgcg
      841 acgagcgcgc aggggcgctt cagcgaggtg gtcgggcaca tgcccactga tcgtgcagca
      901 catatgcctg gcgcgcagcc gcacggcgcg cgaagcggca cacacgccac tgtgggcatc
      961 acctgatcag agtcgagtag agccgagcac agccggatca ggaatcgacc ctcagcgccc
     1021 gagcatccac tggcatcttg acctggtcgt cggcgcatga tgccgctgtc ctcgcatgcg
     1081 agttgattta tgacagctga actggcagcg catccaccac tcggaatatg gtcgagatca
     1141 ctcatcgcag ctgtgaggtc gcgccaggat cccggccccg gacgacagcg aaatgcgcag
     1201 cgctgccagg agaggggatc acctacttcg gcgagaacgc gcggtacatt cgggccacgc
     1261 ggcaccgagc accgtacaac gccgacgcca ggcagcccgt aggctccaac gcagcggcaa
     1321 aggcggacac gccagaaacg aatcggaggg gggcggcgcc gccgttgccc ggcgccgcga
     1381 ccgcgaacga gcgcgcatcg acgactactt cgggttgctg atcgccgagg cgaagcgtcg
     1441 gaggcgccag gtcgcacgcg gcgccatcga ggcgcgcgtc gctgcgctgc acgccgagca
     1501 cacggcgaag ctgcgcgacc tgacggcgcg ctacaccctg cgcgtgcggc tcgatccgat
     1561 cgcgctgatc gcgatcgcga tgcgttcgcc gacgccgccc ggcgcgccgc ccggctcggc
     1621 atcgacgccc tcgaggtcca cgccgcgcac ggctacctgc tgcacgagct cctgtcgccg
     1681 ctcgccaacc agcgcggcga cgcgtacggc ggctcgctcg ccaactgcct gcggctcccg
     1741 ctcgaggtgt tcgaggcggt gcgcgcggtg ttccccgagg accggccggt cggcgtccgg
     1801 gtctctcgac cgatcccggc ctcgccggat cgatcctgac ggtcaccgcg atcagcgaat
     1861 ccggcgccga ggacaaggtg ctgatccggg tcccgtagtc cgccctttgt cccggcacgt
     1921 gcccccaccc caggtcgagc tcgcctggga cgatcccaca tcgtccaccc gacgacgaca
     1981 tcccgtcacc atgggccggt tcgccctcac cgaccccatc gtggcccgcc gcacccctcc
     2041 accccacgcc ccgtcgcggc tgcgcactcc cgtaggccgt ctccgcccca ccaccggcga
     2101 aacggcgctt gtcttccttg gctctcctgg ggggtggtat ctctggcgta aagtgcttgc
     2161 ctggacgacc gatcacgtgt ttgatctatc aagctgctct gggggccagc gcaggcgctg
     2221 attcatcggc tgtctggtgg ctcgtgaatg ccacgaggcc cgcctgggcg ctgcacgtgg
     2281 tgaggtgttc ccaggcgaat ggcccgtgtc gctcactgcc ttggcgccca atgcaaacca
     2341 ggagacgcct gtatgtatca gagctaccag ctgattggct atcagacgcc gaccacgaaa
     2401 ctcggtcccg gggacctcgt gaacctggat gcttatgccg atctgatcgg cgacctcaag
     2461 accaacgacg ggtggctgag cgacatcaag aacgccgaca tgaagaagcg gctggcgcgg
     2521 ctcatcgggg tggtcgagca agcccacgcg ctccagggcc agcaggtgag caatgaccag
     2581 acgaccctca aggtcttcct ggtccccgag ttctacttcc ggccgaccgc cgatcagggc
     2641 gtccagtcca gctactccga cgccgaacgc gacgccctgg tcaaggtcct ggatcgatac
     2701 gtcaaggccg agcgcttcaa gcactggctg ttcgtcttcg gcacggtcat ctggcagatc
     2761 caggtcagca agctcatggc caatgcgcag ctccccgacg gcgtgaagct gaacgacgac
     2821 aaggcccagg cctacgtcat caagaacacg acgct
//
LOCUS       DASVOR010001784         1943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_486173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001784 DASVOR010000000
VERSION     DASVOR010001784.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..705
                     /locus_tag="VF516_33175"
     CDS             <1..705
                     /locus_tag="VF516_33175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112646.1"
                     /translation="AEAAAEVAAEGAVPDAAPTAAVVDPAQTASQQAFGDQPAAQQVD
                     LSHGTFSPEDYQAACTAAGTPEKWDDRYYSGHTDASQWHQPYDHGYANTFTLERGHSA
                     SQAIKDFLAGPTIADWRVIALALELDDVRDTLGDRKFDALFGSAIEADDAQIPVAQRL
                     QVSVDLYTIPLANQMKELAEEKDEAILHEQEPAEPAVAAQVQDKPLEAGVAQEPAPEL
                     VAEELGVDREREQEFA"
     gene            762..>1943
                     /locus_tag="VF516_33180"
     CDS             762..>1943
                     /locus_tag="VF516_33180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112647.1"
                     /translation="MTAEPYRDAASHLRDELRRAWLRVEYEIRLGWARGQVNPAAASA
                     GDAVSPTDIGRLFAAARGERPADDAGAQAVLAQYLELHRAVEARIAASIKTSAAVRAR
                     SPLINLMRVFELTPRQWSALMFALLPDADPSLVQAYRHLARDPSCRGLDGRLLAQLVY
                     DTPETRSLMARDLSNASPLVRYQLIDLAPGDSLLFRRIRAASRLVHLLDGTALGLDPE
                     LAELAELSSGAAPGEFPDVAVELATAAIRSSDVVLAVQGQRGLGKKLLLQRAAAHWKQ
                     RQLVIDGKRLAALHPTTLALALRALVRELHLLEAVPVFADIDDVLVRDGDRDELPGFF
                     AGLLDQWSGPIAITINRERMPRIHHRPLVHLTLEVPPLPVRAALWRQAVPSLDAPAAG
                     SL"
BASE COUNT          253 a          808 c          643 g          239 t
ORIGIN      
        1 gcggaggccg ccgccgaggt ggcggccgag ggcgcggtgc ccgacgccgc gccgaccgcc
       61 gcggtggtcg acccggccca gaccgcctcc cagcaggcgt tcggcgacca gccggccgcg
      121 cagcaagtcg atctcagcca cggcacgttc tcgcccgagg actaccaggc cgcgtgcacc
      181 gccgccggca ctcccgagaa gtgggacgat cgctactact ccggccacac cgatgcctcg
      241 cagtggcacc agccgtacga ccacggctat gccaatacct tcacgctgga gcgcggccac
      301 agcgcgtcgc aggcgatcaa ggacttcctc gccgggccga cgatcgccga ctggcgggtg
      361 atcgcgctcg cgctcgagct cgacgacgtc cgcgacaccc tgggcgaccg caagttcgat
      421 gcgctgttcg gctcggcgat cgaggccgac gatgcccaga tcccggtggc gcagcggctc
      481 caggtcagcg tggacctgta cacgatcccg ctcgccaacc agatgaagga gctcgccgag
      541 gagaaggacg aggcgatcct ccacgagcag gagcccgcgg agcccgcggt cgccgcccag
      601 gtccaggaca agccgctcga ggccggcgtg gcccaggagc ccgcccccga gctggtcgcc
      661 gaggagctcg gcgtcgatcg cgagcgcgag caggagttcg cgtagcgcgc tagccggccg
      721 gaccgctcgc accgcggcgg tcgccgccct accatagcga cgtgaccgcc gagccgtacc
      781 gcgacgccgc cagccacctc cgcgacgagc tgcgccgggc ctggttgcgc gtcgagtacg
      841 agatccggct cggctgggcg cgcggccagg tcaaccccgc cgcggcctcc gccggcgacg
      901 cggtcagccc gaccgacatc ggccggctgt tcgccgccgc gcgcggcgag cgccccgccg
      961 acgacgccgg cgcccaggcc gtgctcgccc agtacctgga gctgcaccgc gccgtcgagg
     1021 cccggatcgc cgcgtcgatc aagacctcgg cagccgtccg cgcccgctcg ccgctgatca
     1081 acctgatgcg cgtgttcgag ctgaccccgc gccagtggtc ggccctgatg ttcgcgctcc
     1141 tgcccgacgc cgacccgagc ctggtccagg cctaccgcca cctcgcccgc gacccgagct
     1201 gccgcggcct cgacggccgg ctgctcgccc agctggtcta cgacaccccc gagacccgct
     1261 cgctgatggc gcgcgacctg tccaacgcct cgccgctggt ccgctaccag ctgatcgacc
     1321 tcgcccccgg cgactcgctg ctgttccgcc ggatccgcgc cgccagccgg ctcgtccacc
     1381 tgctcgacgg caccgcgctc ggcctcgacc ccgagctcgc cgagctcgcc gagctgtcgt
     1441 cgggcgccgc gcccggcgag ttccccgacg tcgcggtcga gctcgccacc gccgcgatcc
     1501 ggtcgtcgga cgtcgtgctc gccgtccagg gccagcgcgg cctgggcaag aagctgctgc
     1561 tccagcgcgc cgccgcgcac tggaagcagc gccagctcgt gatcgacggc aagcggctcg
     1621 ccgccctcca cccgaccacg ctggccctcg ccctgcgcgc cctggtccgc gagctccacc
     1681 tgctcgaggc cgtcccggtg ttcgccgaca tcgacgacgt gctggtccgc gacggcgatc
     1741 gcgacgagct ccccggcttc ttcgccggcc tgctcgacca gtggtccggt ccgatcgcga
     1801 tcacgatcaa ccgcgagcgc atgccgcgga tccaccaccg cccgctcgtc cacctcaccc
     1861 tcgaggtccc gccgctgccg gtccgcgccg ccctgtggcg ccaggccgtg ccgtcgctcg
     1921 acgcgcccgc cgccgggtcg ctc
//
LOCUS       DASVOR010001785        13901 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_107062,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001785 DASVOR010000000
VERSION     DASVOR010001785.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13901
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107062"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VF516_33185"
     CDS             <1..868
                     /locus_tag="VF516_33185"
                     /inference="COORDINATES: protein motif:HMM:NF012892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX8112648.1"
                     /translation="RGEVVAMTGDGVNDAPALVRADVGVAMGITGTDVAKEAAKIVVT
                     DDNFGSIVAAVEEGRVVYRNIKKAVLLLMSTSFAEVVVLLGTVMFGYPPPFAAVQILW
                     NNLVTEGVITVNLIMDPAEGDEMRQPPISTKEPLITRTMWKRIAVMTPTIAAVTLGWF
                     MVRLAMGVPFAVVRTEAFTLLAVCEWFNVLNCLSDHRSAFSFDVFKDKWLVAGLVVGN
                     LLQLGVIYIPAANYVFHTVPIPLVEALSIGCAASIVLWVEELRKLIVRLRNRRPPATQ
                     PSAARVLPAHET"
     gene            complement(904..2106)
                     /locus_tag="VF516_33190"
     CDS             complement(904..2106)
                     /locus_tag="VF516_33190"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX8112649.1"
                     /translation="MIWAVLGLLVVGGLVLAWWKNRSPLTVEASRVTRGVFERFVEED
                     GRARVRDRYVVSSPLAATVERTRFHVGDAVATGDVIAVLHPLPSGLLDARSRAELEQR
                     AGAADAALGRARTVLARSTATLEHANAELARARELLSKKAIPAVEAERAELEARVAAR
                     ARDEAAFAVHVGEHELEVARTALGLATRARSSPERFAIESPIAGRIRRIAQESEGAVG
                     PGTPLLELGDPSALEVVVDLLSTDAVQIEPGDEASILRWGGPEAVTARVRLVEPVAHA
                     KTSALGVEEQRVDVLLDVTGSAPTWNRVGDGYRVDARILVQRMRDAVLVSTGALFREG
                     GEWAAFVVVDGHAAKRSVRLKAYGPTQSAVESGLQEGDLVIEQPPEGLQPGVRLQATA
                     GSVTDHGG"
     gene            complement(2130..4493)
                     /locus_tag="VF516_33195"
     CDS             complement(2130..4493)
                     /locus_tag="VF516_33195"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8112650.1"
                     /translation="MNYLRRKLVRDVQALRWQIVTIALVIAAGVALLVAAFGAYRSLL
                     DARARFYAETQFADVFARLARAPAEVADQLTAIDGVSAVETRLTFDAPVDLEGVGERV
                     AVRVISLVPESRRPVSRLLVQTGRMPEPRTTDEAVVNEAFARARRLAIGDRLAVVLNG
                     HRDEVVIVGTVLSAEHMAAFQGGEIIPDNARFGILWLPEDAMASAYQATGAFNEVALH
                     LAPGASEPRVIAAVDRVLGPYGGYGAYGRAEQPAHRFIDSELGELEVEATVLPVIFLG
                     VAAFLLNGVLARIVANERTQIATLRALGLRIAPIVRHYLSLAAITATLGALAGIAAGA
                     GVGSLMIASYARFFRFPSLAFRVDGSIIAVALVVSVGAGLIGAFVSVRRIVALAPAEA
                     LQPEAPATFRASWLESVPGVRRLAATQRLVVRHIAARPVRTASAVLGIAAAMAVLVVG
                     AFWTDSMNALLAHEFRDVRRQDATVTFHDAVDVGALAELRHIPGVRVVEGVRVVPIRL
                     SHARTTKRVELLGLAAGARLHRLVATDGSEVALPAEGLVLSRHLARRLGVGAGDVVQV
                     DVLEKTRAHGELRIAMVVDELVGLGAYMNLDGVARLIGEAPSVSSALLTIEPGSAPAL
                     HEALGRAPRVATVSIKANAVRLFDESMKAIVTFFSLLLTVFGAAIVIGVVYNAARILV
                     AERARELATFRVIGFTGADVSEALLVELGVQVALGIPLGALFGYGLSAIAVQLFGPED
                     LSIPLVLGARTWALAIAVVLGASVASALLVRRRLDRLNLVEVLKVRE"
     gene            complement(4490..5275)
                     /locus_tag="VF516_33200"
     CDS             complement(4490..5275)
                     /locus_tag="VF516_33200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8112651.1"
                     /translation="MPSWPSACSRDAFDRDPRPVDVPQPGPPVPVLQARGITKVYGTG
                     ASAVHALRGVDLDLFAGEIVVLLGPSGSGKSTLFNILGGLDIPTAGDLVYRDHDLVHA
                     DDAELTRYRCEHVGFVFQFYNLVTALTARENVGMVAELVERHMDPVEALRLVGLSDRL
                     DHFPSQLSGGEQQRVAIARAVAKLPDLLLCDEPTGALDVETGRLVLDALLEVNRTLET
                     TTALITHNAAIAGIADRVLRFSKGRIAAIERSARRISPAELDW"
     gene            5648..6655
                     /locus_tag="VF516_33205"
     CDS             5648..6655
                     /locus_tag="VF516_33205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112652.1"
                     /translation="MLAKDPTTRPLAELLMPSLPLVALGAWLPDEKFFKAGSGNTQNH
                     VLKMAEYVGKGQERFIVSHDATLRQLGSYRKFHAVLKDPGRLPKGWWDKAYRGDCPRG
                     EHPADCAMGLATTLTDLMLLGDKQIGSKATKALGKRIALPEAVTTRVEQIGLYFFMMS
                     HYVADAHMPCHTDARPLARYNGKLHMQWEEYVDKLVAEFPDPAKIPTLQGGVLLDRAA
                     EAIPLDLPAMIPELKGDVWNEVISICRASFAVNCIVASPDKYPLVKTDHPRKDESDLP
                     ADAPMPSFQELFKGKDALRDELSQAILHDAVLGVAKTWKHVWQSVTKFSRNLHAELPD
                     G"
     gene            complement(6670..8121)
                     /locus_tag="VF516_33210"
     CDS             complement(6670..8121)
                     /locus_tag="VF516_33210"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8112653.1"
                     /translation="MVSPDDSAEHLAAGEHSHHAVGLRRNTLSFTETLAQSIANIAPT
                     ATPAINLQLVFACSGNGTWLTYVLATVGLLFIGVCINQFARQSATPGSLYAYVVKGLG
                     PTAGFLVGWGLILAYLATGMATVAGAANYATTVCEKADLHIGPYPLYAIIAGLVWLVA
                     YRDVRLSARMMLLLEVVSVGLILTLGVLLLSHKGFRLDMDQLTLAGMDLNGLRGGMVL
                     AIFSFVGFESATALGAEAREPRKRIPQAVLRSTLITGAFFVVMSFIMVSGFRGSATPL
                     DKAPAALDAMAEIAHAPVLGVLVSIGAVVSFFACSLACVTAVSRILLAMSRDGVFHAS
                     LGKAHQTNATPHIAATLSAVVLFLVPVAMSLGGIEKLDIYNLCGTIATYGFLVAYILI
                     AVGAMRSAARQRASLAMVVVVGVAGILFMGLAVMGSVYPWPDAPFDRLPPIFVAYMVI
                     GLVWMIYDRRRTRAAAGQAASSEGGSQRPSSPG"
     gene            8679..9158
                     /locus_tag="VF516_33215"
     CDS             8679..9158
                     /locus_tag="VF516_33215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112654.1"
                     /translation="MTIAALVLGTGDARGEDEAATPLNYVVVGVGTTAIHRPSGGPVS
                     GWQHDVAPIVGYGRFVYETIALELDIGPTYVRGDYAGTSLVPGVVWAFSEYAYAAARF
                     VVPVDPELDLGLYPGVGLVHTFQNGIGVFAEGNVFSYVGRGTPDLGVALSAGALYSF"
     gene            complement(9159..10076)
                     /locus_tag="VF516_33220"
     CDS             complement(9159..10076)
                     /locus_tag="VF516_33220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8112655.1"
                     /translation="MRAVRIHSYGHSDQVQLEDIAVPSPGVGDVLVGIHAVGVNPVDW
                     KIREGLLAKGAPRALPFTLGQDFAGKVISLGESVGGVEASDTVYGFANGAYAEYAVVS
                     PSMIASKPRTVHDTTAAALPTPGLTALQTVRDAIQPREGQTVLIHGAAGGVGSIATQL
                     CLSRSVRVAATSASRDAAYLHGLGVAEIIDYHTERFEDRVKQVDAVIDLVGGDTLARS
                     LAVLRKGGVVATTVGPLDSAELTRRGLRGVHIVMQKNASDLEELARLVDEGIVKPLPV
                     RVMTLAEARAAQDLIQSGSASEKIVLEVI"
     gene            10223..11179
                     /locus_tag="VF516_33225"
     CDS             10223..11179
                     /locus_tag="VF516_33225"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8112656.1"
                     /translation="MASRTHARDAAPIRIGIVGLGKIARDQHIPSIGADPQFELVAAA
                     TTAGTSPVDIPVYATLGEMIAATPELQAIALCTPPQVRCALAREALAHGLHVMLEKPP
                     GASVGEVEELAARARELHLALFATWHSREAPAVAKACEWIAGRTVRRGAVRWKEDVRT
                     WHPGQPWIWQPGGLGVFDPGINALSIVTRLLSRRLFVTRAVLYVPANQHTPIAVELAL
                     ADGAGAAVDVELDFRRSGHEQWDIQLETDAGLLALRGGGAQLEIDGAPVVAAALQEYP
                     RLYERFAALVRLRAVDVDLAPLKLIADAFLIGQRVTVEPFEQ"
     gene            complement(11211..12710)
                     /locus_tag="VF516_33230"
     CDS             complement(11211..12710)
                     /locus_tag="VF516_33230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112657.1"
                     /translation="MARLNWLIAVAATACGGSDGSVDHGASVLELHAGPSRAGAYVDA
                     AFTHAAAATLHRDPTFTATYTGSVFAQALYLDRGGARDLVIAATEMNEVTAFDPTGAV
                     VWRVELGAPQPMPNAAEGASCGNISPLGITGTPVIDPASGTLYLDAMIRDSNAAHHRI
                     FALSVDDGSTRPGWPVDVGTALKGTATPFVPAPQNQRGGLTLIGGRVYVPYGGHSGDC
                     GNYHGWVVAVPVDRPSAPTAFESTSTRAGDWTPGGVSSDGTSVFTVFGNGGGTTWGHS
                     EAALRLGAGATFSGNAPDYWAPTNWQTLDQGDTDLAGPAMPLDLPGATPSALLVVFGK
                     DGNVYLLARNNLGGITAPLAQLHAASGVILNTSAMYRTANGTYVVFRGHGANGCPGLA
                     GSSNLVSVKIAPGDPPQLSLAWCAGNGGSGSPIATTTDGTSNALVWVAGAEADQRLHG
                     YDGDTGAVVFAGGGAGDAMISTSRFITPIVAKGRIFVAADGLLYAFTTH"
     gene            12771..13673
                     /locus_tag="VF516_33235"
     CDS             12771..13673
                     /locus_tag="VF516_33235"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8112658.1"
                     /translation="MERSRLSLSGLVLLGLVTLAWGLSWPVIKLVLTEVPPLTFRAIC
                     LVAGGAGVLALARLAGQSLRVPAHAWSRLLAIAACNVIGWNVFLIYGIAMLSSGRAAL
                     IGYTMPLWSTLISVWLLGERLTARRGASLVLGMSGVVVLLAADLAGMTSAAGGVVLML
                     AAALSWGLGVVLLKRFALPIPAACLTGWMMLAGSAPIVAGALVLEHDRWRPVSATVGL
                     GLAYSVIIAFMFGYWAWNRLVLMVPVAVSSVSILATPVIGVLSGLWLLHEPLTWREIA
                     AGACILGAIALVLGSSPARRAGDR"
     gene            complement(13679..>13901)
                     /locus_tag="VF516_33240"
     CDS             complement(13679..>13901)
                     /locus_tag="VF516_33240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112659.1"
                     /translation="VAVDASGVVVVTGSLGADLIARKYTPAGALMWERRLAAARGNAV
                     AIDAHGDVLVCGAITAAGNTDGLVAVFLQ"
BASE COUNT         2099 a         4730 c         5002 g         2070 t
ORIGIN      
        1 gcgcggtgag gtcgtcgcga tgaccggaga cggcgtcaac gatgcgcctg ccctggtccg
       61 cgcggatgtc ggcgtcgcca tggggatcac cggtaccgac gtcgccaagg aggccgccaa
      121 gatcgtcgtc accgatgaca acttcggatc gatcgtggca gccgtggagg agggacgcgt
      181 cgtctaccgc aacatcaaga aggccgtgtt gctgctgatg tcgacgtcgt tcgccgaggt
      241 cgtcgtgttg ctcggtacgg tgatgttcgg ctatccgccg ccgttcgcgg ccgtccagat
      301 cctctggaac aacctggtga cggagggcgt gatcaccgtg aacctgatca tggatcccgc
      361 cgagggggac gagatgcggc agcctccgat ctcgacgaag gagccgctga tcacgcgcac
      421 gatgtggaag cgcatcgccg tgatgacgcc gacgattgct gccgtgacgc tcggctggtt
      481 catggtccgg ctcgccatgg gcgtgccgtt cgcggtcgtg cgcaccgagg cgttcacgct
      541 gctcgccgtg tgcgagtggt tcaacgtgct caactgcctg tcagaccacc gctcggcctt
      601 cagcttcgac gtgttcaagg ataagtggct cgtcgctggt ctcgtggtcg ggaacctgct
      661 ccagctcgga gtcatctaca tcccggcggc gaactacgtc tttcacacgg tgccgatccc
      721 gctcgtcgag gcgctctcga ttggctgcgc ggcaagcatc gtcttgtggg tcgaggagct
      781 gcgcaagctg atcgtgcggc ttcgcaatcg ccgcccaccg gcaacgcagc catccgccgc
      841 tcgcgtgctt ccagcgcacg aaacctgagc gcgagccccc agccgatcca cccggtcctg
      901 cgctcagccg ccatggtcgg tcaccgatcc ggcggtcgcc tggagacgca cgcctggctg
      961 cagcccctcg ggtggttgct cgatcacgag gtcaccttcc tgcaggccgg actcgacggc
     1021 ggactgcgtc gggccgtacg ccttgagccg caccgatcgc ttcgccgcgt ggccgtcgac
     1081 cacgacgaac gcggcccact cgccaccctc gcgaaacagt gcgccggtcg agacgagcac
     1141 ggcatcgcgc atccgctgga cgaggatgcg cgcatcgacg cggtagccat cgccgacgcg
     1201 attccaggtc ggagcgcttc ccgtcacgtc gaggagcaca tcgacgcgtt gctcctcgac
     1261 gccgagcgcc gaggtcttcg cgtgggcgac aggctcgacg aggcgcacgc gcgcggtgac
     1321 cgcctcgggt ccgccccatc gcaggatcga tgcctcgtct cccggctcga tctgcacggc
     1381 atcggtgctc agcagatcca ccaccacctc gagcgcgctc gggtccccga gctcgagcag
     1441 cggcgtaccg ggacccacgg cgccctcgct ctcctgcgcg atgcgccgga tccgaccggc
     1501 gatcggcgac tcgatggcga accgctccgg tgaggaccgc gcgcgcgtcg cgaggccgag
     1561 cgccgtgcgc gcgacctcga gctcgtgctc gccgacgtgg acggcgaacg ccgcttcgtc
     1621 gcgtgcgcgc gctgcgaccc gcgcttcgag ctcggctcgc tcggcttcga ctgccgggat
     1681 cgccttcttg ctcaggagct cgcgtgcgcg ggccagctcc gcattcgcat gctccagggt
     1741 ggctgtcgag cgtgccagga cggtgcgtgc gcgtccgagt gcggcgtcgg cggcaccggc
     1801 gcgctgctcc agctcggcgc gggaacgggc gtcgagcaga ccgctcggca gcggatgcag
     1861 caccgcgatc acgtcgccgg tggcgacggc atcgccgaca tggaaccgcg tgcgctcgac
     1921 ggtcgcggcc agcggcgacg agaccacgta gcggtcgcgc acgcgcgcac gtccgtcctc
     1981 ctcgacgaac cgctcgaata cccctcgggt gacgcgagat gcctcgaccg tcagcggcga
     2041 gcgattcttc caccaggcga gcaccaggcc gccgaccacg agcagcccga ggaccgccca
     2101 gatcatccga cgcgcttttc ctttgagact cattctcgca ccttcaggac ttcgaccagg
     2161 ttcagccgat cgagacggcg gcggaccagg agcgcgctgg cgaccgaggc gcccagcacc
     2221 acggcgatcg ccagcgccca ggtccgggcg ccgagcacga gcggaatcga caggtcctcg
     2281 ggtccgaaca actggaccgc gatcgccgac agcccatatc cgaacagcgc accgagcggg
     2341 atgccgagcg cgacctgcac gccgagctct acgagcaggg cctcggagac atcggcgccg
     2401 gtgaagccga tgacccggaa cgtggcgagc tcccgggcgc gctccgcgac gaggatccgc
     2461 gccgcgttgt acacgacccc gatgacgatg gcggcgccga acaccgtgag cagcaacgag
     2521 aagaacgtga cgatcgcctt catcgactcg tcgaacaggc gcaccgcatt ggctttgatc
     2581 gagaccgtgg cgacgcgcgg cgctcgcccg agcgcctcgt gaagcgcggg cgcgctccca
     2641 ggctcgatcg tcagcagcgc cgaggacacg ctcggagctt ctccgatcag gcgggcgaca
     2701 ccgtcgaggt tcatgtaggc gcccagcccg acgagctcgt cgacgaccat cgcgatgcgc
     2761 agctcgccgt gggcccgcgt cttctcgagg acgtccacct gaacgacatc ccccgcgccg
     2821 acaccgagac gacgcgcgag atggcgcgac agcacgagcc cttcggccgg caatgcgacc
     2881 tcgctgccgt cggtagcgac cagccggtga agtcgcgcgc cggcggcgag cccgagcagc
     2941 tcgactcgct tcgtggtccg tgcatgcgag agccggatcg ggaccacccg gacaccttcc
     3001 acgacgcgaa ccccggggat gtggcgaagc tcggcgaggg cgcctacgtc gacggcgtca
     3061 tggaaggtca cggtcgcgtc ctggcgccgc acatcccgga actcgtgcgc gaggagcgcg
     3121 ttcatcgagt cggtccagaa cgcgccgacc acgagcaccg ccatcgcggc ggcgatcccg
     3181 aggacagcgc tcgcggtgcg cacgggccgc gcagcgatgt gccgcacgac gagccgctgc
     3241 gtcgccgcga gccgtcgtac cccgggtacg ctctcgagcc agctcgcgcg gaacgtcgcc
     3301 ggggcctcgg gttgcagcgc ctccgccggc gcgagcgcga cgatcctccg caccgacacg
     3361 aacgcgccga tcagccccgc acccacgctg accacgagcg cgaccgcgat gatcgagccg
     3421 tcgacgcgga acgccagcga tgggaaccgg aagaaccgcg cgtacgacgc gatcatcaag
     3481 ctcccgacgc cggccccggc tgcgatcccc gcaagcgcac ccagcgttgc cgtgatcgct
     3541 gcgagcgaga ggtaatgacg cacgatcggt gcgatccgga gccccagcgc gcgcaaggtg
     3601 gcgatctgcg tgcgctcgtt cgcgacgatg cgcgcgagca caccgttcag caggaatgcg
     3661 gccacgccca ggaagatcac ggggagcacc gtcgcctcga cctcgagctc acccagttcg
     3721 ctgtcgatga accgatgcgc aggctgctcg gcgcggccgt aggcaccata cccgccgtac
     3781 ggcccgagca cgcggtcgac ggcggcgatc acgcgtggct cgctcgcgcc gggcgccagg
     3841 tgcagcgcga cctcgttgaa cgcacccgtc gcctggtacg cggacgccat ggcatcttcg
     3901 ggcagccaca ggatcccgaa gcgagcgttg tccgggatga tctcgccacc ctggaacgcg
     3961 gccatgtgct cggcggacag cacggtcccc acgatgacga cctcgtcgcg gtgtccgttg
     4021 agcacgaccg cgaggcggtc gccgatcgcg agccggcgag ccctcgcgaa cgcctcgttg
     4081 accacggcct cgtcggtggt ccgcggttcc ggcatgcgcc cggtttgcac cagtagacgc
     4141 gataccggcc gccgactctc gggaacgagc gagatcacgc gcaccgcgac gcgctcgccg
     4201 accccttcga gatcgaccgg tgcgtcgaac gtgagccgcg tctcgaccgc ggatacccca
     4261 tcgatggcgg tgagctggtc cgcgacctcg gcgggtgccc gtgcgagccg cgcgaagacg
     4321 tccgcgaatt gcgtctcggc gtagaagcgt gcgcgcgcgt cgagcagcga tcggtatgcg
     4381 ccgaacgccg ccacgagcag cgcgacgccc gcggcaatga ccagcgcgat cgtgacgatc
     4441 tgccagcgca gcgcctggac atcgcgaacg agcttgcgcc ggaggtagtt caccagtcga
     4501 gctccgcagg tgagatccgt cgcgcgctgc gctcgatcgc cgcgatccgt cccttggaga
     4561 atcgcagcac gcggtccgcg atcccggcga tcgcggcatt gtgcgtgatc agcgcggtgg
     4621 tggtctcgag tgtccggttg acctcgagca gcgcgtcgag caccagtcgc ccggtttcga
     4681 catcgagcgc gccggtcggc tcgtcgcaca gcaggagatc cggcagcttg gcgaccgcgc
     4741 gcgcgatcgc gacgcgctgt tgctcgccgc ccgacagctg cgacgggaag tggtcgagcc
     4801 gatcgctcag gcccacgagc cggagcgcct cgacgggatc catgtggcgc tcgacgagct
     4861 cggcgaccat gccgacgttc tcgcgcgcgg tcagcgcggt caccaggttg tagaactgga
     4921 acacgaaccc cacgtgctcg cagcggtacc gggtgagctc ggcgtcatcg gcgtgcacga
     4981 gatcgtgatc gcggtacacc agatcgcccg cggtcgggat gtcgagccca cccaggatgt
     5041 tgaacagcgt cgacttcccg ctgcccgacg gcccgagcag caccacgatc tctccggcga
     5101 acaggtcgag atcgacgccc cgcagggcat gcacggcgga ggcgcccgta ccgtagacct
     5161 tggtgatgcc gcgggcctgg aggaccggaa ccggcggccc gggctgcggc acgtccaccg
     5221 gccgcgggtc gcgatcgaac gcatccctgg agcacgcgct cggccagctc ggcatgaacg
     5281 catcgacttg cagaacctat gccctcgtct ccgaggagcc ccatgtccac gacaccttct
     5341 cccgatcgca gattcatcgt tgccctcacc gccgcggtcg cctctccgac cctggcgatc
     5401 ggagtgacga ccggagccct gctcggctgg atgtgggccg gggcgagaac gcgaatggct
     5461 tgcggtcgac gggtgatctc gcgcgtggat tgccggcaga tcgaccgcga ggccgccgac
     5521 cgtccgcggt ccagctcttg ctccacttcc atgccggtga cgccacggcg catgtccggg
     5581 aaccgtcacg gaggagaaag ctatgaagaa gctaggtcac gctgccatcg cgacgcgcgc
     5641 cgtcggtgtg ctcgccaagg atccgacgac gcgaccgctg gcggagctgt tgatgccgag
     5701 cttgccactg gtcgcgctcg gcgcgtggct gccggacgag aagttcttca aggccggcag
     5761 cggcaatacg cagaatcacg tcctgaagat ggccgaatac gttggcaagg gccaggaacg
     5821 gttcatcgtg agtcacgacg cgacgctccg ccagctcggg agctaccgga agtttcacgc
     5881 tgtgctgaag gacccggggc gcctgcccaa gggctggtgg gacaaggcgt accgcgggga
     5941 ctgtccgcgc ggcgagcatc ccgccgactg cgccatgggc ctggcgacca cgctgaccga
     6001 cctgatgctc ctcggggaca agcagatcgg aagcaaggcg accaaggcgc tggggaagcg
     6061 gatcgcgctt ccggaagcgg tcacgactcg ggtcgagcag atcggcctgt acttcttcat
     6121 gatgagtcac tacgtggccg acgcacacat gccctgccac acagacgcgc gcccgctggc
     6181 caggtacaat ggcaaactcc atatgcaatg ggaagagtac gtcgataaac tggtggcaga
     6241 gtttccggat ccggcgaaga tcccgacgct gcaggggggc gttctcctcg accgcgccgc
     6301 ggaggcgatc ccgctcgatc tcccggccat gatcccggag ctgaaaggcg acgtctggaa
     6361 cgaggtcatc tcgatctgtc gagcctcttt tgctgtcaac tgcatcgtcg catctccgga
     6421 caagtacccg ctggtcaaga ccgatcaccc gcgcaaggac gagagcgatc tgcctgccga
     6481 tgcgccgatg ccttcgttcc aggagctatt caaggggaag gacgcactgc gagacgagct
     6541 gagccaggcc atcctccacg atgccgtgct gggcgtcgcc aagacgtgga agcacgtgtg
     6601 gcagtcggtg accaagttct ccaggaacct ccacgccgag cttccagacg gctgagcgct
     6661 ggaacgcgat caccccgggg aacttggtcg ctgcgacccg ccttccgagc tcgcagcttg
     6721 cccagcggcg gcgcgcgtac gccgccggtc gtagatcatc cacacgagac cgatcaccat
     6781 gtaggcgacg aagatcggcg gcagcctgtc aaagggggca tccggccaag gatagacgct
     6841 gcccatgacg gcgaggccca tgaacaggat cccggccact cccacgacca cgaccatggc
     6901 cagcgaggct cgctggcgtg ccgcggaacg catcgcgccg accgcgatga ggatataggc
     6961 gacgagaaat ccgtacgtgg cgatcgtgcc gcagaggttg tagatatcca gcttctcgat
     7021 cccgcccaga ctcatcgcga ccgggacgag gaacagcacc accgccgaga gcgtcgcggc
     7081 gatgtggggc gtggcgttgg tctggtgcgc cttgccgagg gaggcatgga agacgccgtc
     7141 gcgggacatg gcgagcagga tgcgggagac ggcggtgacg cacgcaagcg agcacgcgaa
     7201 gaagctgacg accgcgccga tcgagaccag gaccccgagc acgggcgcgt gggcgatttc
     7261 ggccatcgca tcgagcgcgg cgggagcctt gtccaggggc gtggcgctgc cccggaagcc
     7321 gctgaccatg atgaacgaca tgaccacgaa gaacgcgccg gtgatcagcg tgctgcggag
     7381 caccgcttgc gggatccgct tgcgcggctc gcgggcctcc gcgcccagcg ccgtcgcgct
     7441 ctcgaagccg acgaagctga agatcgccag caccatgccg ccgcgcaggc cattcagatc
     7501 catccccgcg agggtgagct ggtccatgtc caggcggaag cccttgtgcg agagcagcag
     7561 gacgcccagg gtcaggatca ggccgaccga gacgacctcc aggagcagca tcatccgggc
     7621 ggacagccgc acgtcgcgat aggcgacgag ccagaccagg ccggcgatga tcgcatagag
     7681 cgggtagggc ccgatgtgca gatcggcctt ctcgcagacg gtggttgcgt aattggcggc
     7741 tcccgcgacg gtggccattc ccgtggccag atacgccagg atcaggcccc agccgacgag
     7801 gaagccggcg gtcggcccga gacccttgac cacgtaggcg tagagcgagc ccggcgtcgc
     7861 gctctgccgg gcgaactggt tgatgcagac gccgatgaac agcaggccga ccgtcgccag
     7921 cacgtaggta agccaggtgc cgtttccgga gcacgcgaag acgagctgga ggttgatcgc
     7981 cggcgtcgcg gtcggggcga tgttggcgat cgactgcgcc agggtctcgg tgaaggacag
     8041 ggtgttgcgg cgcagaccga cggcgtggtg gctgtgctcg ccggcagcca ggtgctcggc
     8101 ggagtcgtct ggtgaaacca tggggcggtc gtcctttccg gagctgggcg cgcgcgcatc
     8161 gtagcgaatc cgcgcggcgc gcccgtggga gatgtttaga gaggttcgcg ttctcgcgca
     8221 cggatcggca tggcgagatc ccgctcaccg tgcgggtaga caatcgtgtt ctggtttggg
     8281 atcatcgcag tccgcgcgcc cggccttgcg tgcctcgaag atcgcagcga ggtgccgccc
     8341 ggtgggttcc accgcttgcc gcgttccgcc agcttcgtcc gccgtcttcg agcgcgggct
     8401 cggctgtcga ggacgggtgg atgacagctc acctgtggtg tctggctgtg tttcacgcga
     8461 tcgcggatgt gcccgaacgt gccggagcgc atccacgcga gttggggtat ccagtcacag
     8521 tatcgcgctg agccagtaga tggtcagtgt gctccgtgat cgggagattg cgatcgtttc
     8581 cgtgcccagg agctattgcc atgttcacaa tacgggattc atgctctcgc tgcttgccgc
     8641 cgcggcgcga tcgaccgggc ctggttcgcc ttgcggtcgt gaccatcgcc gcgctggtgc
     8701 tcgggaccgg cgacgcgcgc ggcgaggacg aagcggccac gccgctgaac tacgtggtcg
     8761 tgggcgtcgg gaccaccgcg atccaccgtc cgtcgggcgg cccggtgagc ggctggcagc
     8821 acgacgtcgc tcccatcgtg ggatacggcc ggttcgtgta cgagacgatc gcgctcgagc
     8881 tggacatcgg cccgacgtat gtccgcggcg actatgccgg cacatcgctc gtgccgggcg
     8941 tggtctgggc gttctccgag tacgcctacg ccgcggctcg cttcgtcgtc ccggtcgatc
     9001 ccgagctcga cctgggcctc taccccggcg tcggtctggt ccatacgttc cagaacggga
     9061 tcggggtctt tgcggagggc aacgtgttca gttatgtcgg ccggggcact ccggatctcg
     9121 gcgtggcgct gagcgcgggc gcgctgtact cgttctgatc agatgacctc gagcacgatc
     9181 ttctccgacg cgctgccgga ctggatcagg tcctgggcgg cgcgcgcctc ggcgagcgtc
     9241 atcacgcgca ccgggagcgg cttgacgatg ccctcgtcga cgaggcgcgc cagctcctcg
     9301 aggtcggacg cgttcttctg catgacgatg tgaacgccgc gcaggccgcg tcgcgtgagc
     9361 tcggcggagt cgagcgggcc gacggtggtc gccacgacac cgcccttgcg aagcacggcg
     9421 agcgaccggg ccagggtgtc gccaccgacg agatcgatga cggcgtcgac ctgcttcacg
     9481 cggtcctcga accgctcggt gtggtagtcg atgatctcgg cgacgccgag gccgtggagg
     9541 taggcggcgt cgcggctcgc cgaggtggcg gcgacgcgca cgctccggga caggcacagc
     9601 tgggtcgcga tcgagccgac gccgccggcg gcgccgtgga tcaggacggt ctggccctcc
     9661 cggggctgga tcgcgtcgcg gacggtctgc agcgcggtca gcccgggggt cggcaaggcg
     9721 gcggcggtgg tgtcgtggac ggtgcgcggc ttcgacgcga tcatggacgg cgagacgacg
     9781 gcgtactcgg cgtacgcgcc gttcgcaaag ccgtagaccg tgtcgctggc ctcgacgccc
     9841 cccacgcttt ctccgaggct gatcaccttg ccggcgaagt cctggccgag ggtgaacggc
     9901 aaggcccggg gcgccccctt cgccagcaga ccttcgcgga tcttccagtc gacggggttg
     9961 acccccacgg cgtggatccc gacgagcacg tcaccgacgc cgggactcgg cacggcgatg
    10021 tcctcgagct ggacctgatc ggaatgtccg taggaatgga tcctgaccgc tctcatggag
    10081 cgacaccgtg caacaccggc gccagccgct gcccgggacg cgcgggcgat gatcgaggtg
    10141 ctgcgccgag cgcggcgggc gcggcttgcc ggcgggcacc gggtcggaga tagtcgccgc
    10201 gccgctgccg gtggagaagc gcatggcatc gaggacccac gccagggacg ccgcgccgat
    10261 ccggatcggg atcgtggggc tggggaagat cgcgcgggat cagcacatcc cgtcgatcgg
    10321 cgcggacccg cagttcgagc tggtcgcggc ggcgacgacg gcggggacct cgcccgtcga
    10381 catcccggtg tacgcgacgc tgggcgagat gatcgcggcc acgccggagc tgcaggcgat
    10441 cgcgctgtgc acgccgccgc aggtgcggtg cgcgctggcc cgcgaggcgc tggctcacgg
    10501 gctgcacgtg atgctggaga agccgcccgg ggcgagcgtc ggcgaggtcg aggagctcgc
    10561 cgcccgcgcg cgcgagctgc acctggcgct gttcgcgacc tggcattcgc gcgaggcgcc
    10621 ggcggtggcg aaggcgtgcg agtggatcgc gggccgcacg gtgcgccggg gcgcggtgcg
    10681 ctggaaggag gacgttcgca cgtggcaccc ggggcaaccg tggatctggc agccgggcgg
    10741 gctgggggtg ttcgatcccg gcatcaacgc gctgtcgatc gtcacccggc tgctgtcgcg
    10801 ccggctgttc gtgacacgcg cggtgctgta cgtgccggcc aaccagcaca cgccgatcgc
    10861 ggtcgagctg gcgctggccg acggcgcggg cgcggcggtc gacgtcgagc tggattttcg
    10921 ccggtcgggg cacgagcagt gggatatcca gctcgagacc gatgcggggc tgctggcgct
    10981 gcgcggcggc ggcgcgcagc tggagatcga cggtgcgccg gtcgtcgccg cggcgctgca
    11041 ggagtatcct cggctgtacg agcggttcgc cgcgctggtc cggctgcgcg cggtcgatgt
    11101 cgacctcgct ccgctgaagc tgatcgcgga cgcattcctg atcggccagc gggtcaccgt
    11161 cgagccgttc gagcagtgat tcacagcgac agccgcgcct tgttcgcgga tcagtgggtc
    11221 gtgaacgcgt agagcaggcc gtcggccgcg acgaagatcc ggcccttggc gacgatcggg
    11281 gtgatgaacc ggctggtcga gatcatggcg tcaccggcgc cgccgcccgc gaacacgacc
    11341 gcgccggtgt cgccgtcgta gccgtgcagc cgctgatcgg cctcggcgcc ggccacccac
    11401 acgagcgcgt tgctcgtgcc gtcggtggtg gtggcgatcg gggagccact gccgccgttg
    11461 ccggcgcacc acgcgagcga cagctggggc ggatcgccgg gagcgatctt gaccgagacc
    11521 aggttggacg agccggccag gccggggcag ccgttcgcgc cgtggccgcg gaacacgacg
    11581 taggtgccgt tcgcggtgcg gtacatcgcc gaggtgttga ggatcacgcc gctcgcggcg
    11641 tgcagctgtg cgagtggagc ggtgatgcca ccgaggttgt tgcgcgcgag caggtagacg
    11701 ttaccgtcct tgccgaacac gacgagcagc gccgacgggg tcgcgccggg cagatcgagc
    11761 ggcatcgccg gtccggcgag gtcggtgtcg ccctggtcga gggtctgcca gttggtcggc
    11821 gcccagtagt cgggggcgtt gcccgagaac gtggcgccgg caccgagccg gagcgccgcc
    11881 tcgctgtggc cccaggtcgt gccgccgccg ttgccgaaca cggtgaacac gctggtgccg
    11941 tcgctggaca cgccgccggg cgtccagtcg ccggcccggg tcgacgtgga ctcgaacgcg
    12001 gtcggcgcgc tcggccggtc gaccggcacg gcgacgaccc agccgtgata gttgccgcag
    12061 tcgccggagt ggccgccgta gggcacgtag acccggccgc cgatcagcgt caggccgccg
    12121 cgctggttct gcggcgcggg gacgaacggg gtcgccgtcc ctttcagcgc ggttccgacg
    12181 tcgaccggcc agcccggacg cgtggagccg tcgtcgaccg acagcgcgaa gatgcggtga
    12241 tgcgcggcgt tgctgtcgcg gatcatcgcg tcgaggtaga gcgtgccgct ggcggggtcg
    12301 atcaccggcg tgccggtgat gccgagcggg ctgatattgc cgcagctggc gccctcggcg
    12361 gcgttgggca tcggctgcgg cgcgccgagc tcgacccgcc acacgaccgc gccggtcggg
    12421 tcgaacgcgg tgacctcgtt catctcggtg gcagcgatca cgagatcgcg cgcgccgccg
    12481 cgatcgaggt agagcgcctg tgcgaacacc gagccggtgt acgtcgcggt gaaggtgggg
    12541 tcgcggtgga gcgtcgcggc ggcggcgtgg gtgaacgcgg cgtcgacgta cgcgcccgcg
    12601 cgcgacggtc cggcgtggag ctccagcacc gacgcgccgt ggtcgacgga gccgtcactg
    12661 cccccgcagg cggtcgcggc cactgcgatc agccagttca agcgtgccat gcggcgatta
    12721 cagcacggcg cgccggggga cggggcgccg gcaggatacg ctgcggcgcc gtggagcgct
    12781 cccgcctgtc gctgtccggc ctcgtcctgc tcgggctggt cacccttgcc tggggactgt
    12841 cgtggccggt gatcaagctc gtgctcaccg aggtgccgcc gctgacgttt cgcgcgatct
    12901 gcctggtcgc gggcggcgcc ggggtgctcg cgctggctcg cctcgccggg cagtcgctgc
    12961 gcgtgccggc gcacgcgtgg agccgcctgc tcgcgatcgc cgcgtgcaac gtcatcggct
    13021 ggaacgtgtt cctgatctac ggcatcgcga tgctgtcgtc ggggcgggcc gcgctgatcg
    13081 gctacacgat gccgctgtgg agcacgctga tctcggtgtg gctgctcggc gagcggctca
    13141 cggcgcggcg cggggcctcg ctcgtcctcg ggatgagcgg cgtcgtggtc ctgctcgcgg
    13201 ccgatctcgc cggcatgacg agcgccgccg gcggcgtggt gctgatgctg gccgccgcgc
    13261 tgtcgtgggg gctcggcgtc gtgctgctca agcggttcgc gctgccgatc ccggcggcgt
    13321 gcctgaccgg ctggatgatg ctcgccggca gcgcgccgat cgtcgccggc gcgctggtgc
    13381 tggagcacga ccgctggcgg ccggtcagcg cgaccgtcgg gctcggcctg gcctacagcg
    13441 tgatcatcgc gttcatgttc ggctactggg cgtggaaccg gctcgtgctg atggtgccgg
    13501 tggcggtgtc gtcggtctcg atcctcgcga cgccggtgat cggcgtgctg agcggcctgt
    13561 ggctgctcca cgagccgctg acctggcgcg agatcgccgc cggtgcgtgc atcctcggcg
    13621 cgatcgcgct cgtgctcggg tcatcccccg ctcggcgcgc cggcgatcgg tagcgcggct
    13681 actgcaggaa cacggcgacc agcccgtcgg tgttgccggc ggcggtgatc gcgccgcaca
    13741 ccaggacgtc gccgtgggcg tcgatcgcga ccgcgttgcc gcgcgccgcg gcgagccggc
    13801 gctcccacat cagggcgccg gccggcgtgt acttgcgcgc gatcaggtcg gcgccgaggc
    13861 tgccggtgac cacgacgacg ccggaggcgt cgaccgcgac g
//
LOCUS       DASVOR010001786         8493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_311052,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001786 DASVOR010000000
VERSION     DASVOR010001786.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8493
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311052"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2409
                     /gene="fdhF"
                     /locus_tag="VF516_33245"
     CDS             <1..2409
                     /gene="fdhF"
                     /locus_tag="VF516_33245"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HEX8112660.1"
                     /translation="REQPRADLSHPAIAVNLDACIQCTRCVRACREEQVNDVIGYAFR
                     GEHSKIVFDLDDPMGGSTCVACGECVQVCPTGALMPARDVALAKADRTVDSVCPYCGV
                     GCLLTYHIRDNKILHVQGRDGPANQSRLCVKGRYGFDYVHHKQRLTTPLVRKPGVPKT
                     KDFTVHPEHWHEAFREATWEEALELAASGLRELRDRHGKQALAGFGSAKCTNEEAYLF
                     QKLVRTGFGSNNVDHCTRLCHASSVAALMEGINSGAVSNQVRDVEKAEVVIVIGANPT
                     GNHPVAATWIKNAAKRGTKLIVADPRRTDLARYATHVLQFHADTDVALLNAMLHTIVE
                     EGLVDEAFVRDRTANFEALRENVQKYSPESMAPICGIPASTIREVARLYATSKGSMIL
                     WGMGISQHVHGTDNARCLIALALSTGQIGRPGTGLHPLRGQNNVQGASDSGLIPMVYP
                     DYQRVDNPEVRSSFERLWGTELDPRPGLTVVEIINAAKQREIRGMYIMGENPAMSDPD
                     LEHAREAIAALDHLVVQDIFLSETAYLADVVLPATAWPEKVGTVTNTDRMVQLGRKAI
                     EPPGDAREDFWIIQELARRMGLPWRYEHPRDVFDEMRRCMRSIAGITWDRLERDSSVT
                     YPCENEGDPGEPVVFVTQFPTATGRGKFVPADIIRAAEQPDDEYPMVLITGRQLEHWH
                     TGSMTRRAGVLDAIEPEPVASFHRNDLERLGVTPGEVVTVESRRGAVSLYARVDDGTP
                     AGAVFMPFCYYEAAANKLTNPVLDPVGKIPEFKYCAVRVARGGEIPKQASYGGGQTPL
                     PGQR"
     gene            2575..2835
                     /locus_tag="VF516_33250"
     CDS             2575..2835
                     /locus_tag="VF516_33250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEX8112661.1"
                     /translation="MVTIELYGVPRMRAGRDEITVEAGCLGDALAALGRECPVLAPSV
                     VDGQRLQPCYVVAINGIQFTADAKHPLRDGDAIVLLSADAGG"
     gene            2837..4660
                     /locus_tag="VF516_33255"
     CDS             2837..4660
                     /locus_tag="VF516_33255"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HEX8112662.1"
                     /translation="MRGGFFGEYLRIDLGSGEAIRVALDPAIARRFIGGVGLGAWLLH
                     QETPRGFDPLGAEAAVIFAFGPLLGTPLTTSAKYALVAKSPLTGRIGDALSSSRFAVA
                     AKRMGVDAIVLTGCAARPSVLLLDEDRASLEAAGDLWGHGLPVSEAARALATRFPGHA
                     FAGIGPAGEHLVRYAGVTNEGRHAGRGGLGAVLGAKRLKAIGVRGVRRVPLADAAKTT
                     ALSKALARRSLGPATEKYRELGTVGNLAAFNRLAALPTRNFQESTFEGAVDVSGEILH
                     ATRRKGRGSCINCTIGCEHFFEVAPGEAPVKAEYENVFALGPLCGIRDPEVVLRASRS
                     CDELGLDTISAGGTIAFAMECAERGLFDGTVFEAQARGLRFGDGARLLALLDDIAFRR
                     GALADLLAEGSRSAAARLGPPAPDFAPHVKGLEIPGYEPRALQSMALGFSVGSRGADH
                     NRSGAYEADFSGQVDRFHVSAESARSAIATEDRAAMLDSLILCKFLRGTLVDFFGESA
                     EMLAAVTGFDCDADELRRAAQRIVLLKRLFNEREGWTCAEDTLPERFFTQSLAKGAAS
                     GAVISRAGLAHSIAAYYEARGLRPDGTVPAETLCANQLDTL"
     gene            complement(4665..5342)
                     /locus_tag="VF516_33260"
     CDS             complement(4665..5342)
                     /locus_tag="VF516_33260"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEX8112663.1"
                     /translation="MLPVPRLGGIVLCGGRSSRMGSPKAWLDFGGEPLLARVVRTVRR
                     ACAPVIVVAAPGQDVPPLPPDVAIARDPVEGRGPLQGIAAGLQALLESPGGSEAVDAA
                     FVSTTDAPFLEPALIARLAALRGGDHDIAVPNMGDYFHPLTAIYACTCLAHIKALLAA
                     DRLRPFFLFERVRTVLCDAPALLADPDLATADPGLRSLENINTPDDYERALQSLGARV
                     GPGPDPE"
     gene            complement(5342..7261)
                     /locus_tag="VF516_33265"
     CDS             complement(5342..7261)
                     /locus_tag="VF516_33265"
                     /inference="COORDINATES: protein motif:HMM:NF011068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein"
                     /protein_id="HEX8112664.1"
                     /translation="MKQEQFLHVVDRDEAERSFRAALGDLSPRRTEVVALHEALGRVL
                     AEDVCSPVDVPGFDRANVDGFAVRAQDTFGASEFAPKKLRLLAARVAMGAVPSAAVAP
                     GSAMAIPTGGVVPRGADAVLMVEHTSMEDDALVVTKALTPGRNISFAGTDVAQGETVL
                     RARDVLTSRETGVLAAMGIARVSVVAQPRVAILSTGDEIVAPGAPIAVGKIYDSNATI
                     LGDAVREQGGTVIPLGVVGDDLEAVKAALARGLAAAEVVLLSGGTSKGPGDLNVRALE
                     AILGPSGIVVHGVALKPGKPICLAVSGGKPVVVLPGFPASAIFTFHEFVAPVIRKLAG
                     RDEQDVARVAARVPYAISSEVGRTEYVLVRLVEDDAGSLVAYPIGKGSGSVTAWSQAD
                     GYFVIPRAVEQLDAREEVSVLATAGARARKDDLVVIGSHCVGLDIILARLRARGLTVK
                     LIAIGSEGGLRAVQRGECDVAGAHLFDTATGAYNQPFLTPDLDLVRGYGRLQGVVFRP
                     GDERFEGKPAEVAFREAAATPGLVMVNRNRGSGTRAIIDRLLGDLRPDGHGVEASSHH
                     AIAAAVAQARADFGVAIDVVARERGLGFAPIAEERYDFFVRRARRDRAAVRAFIEELE
                     RPATRAALRARGLVA"
     gene            complement(7258..>8493)
                     /gene="glp"
                     /locus_tag="VF516_33270"
     CDS             complement(7258..>8493)
                     /gene="glp"
                     /locus_tag="VF516_33270"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEX8112665.1"
                     /translation="RMHGFRERTSVSAAIAILERRTASLGLGVERVGVSAAHGRVAAE
                     AVIAGVDVPSFARSAMDGFAVVGESTFGASAQAPVELALIGETKAGRWFGGTLRTGEA
                     VRITTGAPVPTGADAIVMAEIAEATGDTVRVREAVAPGKHVSTVGEDIRRGDLVVERG
                     RRLRPQDVGVLASLGVAEILLVRRPRVSIVITGDEVLPAGDMPRDALISDADGPMLRA
                     LAERDGAGAIELRYLPDDRDALRALFAAHDGDVLLVSGGSSVGPEDHAPTVLAELGEV
                     AVHGVALRPASPAGFGFLRSGCPVFLLPGNPVSCLCAYEFFAGPTIRALGGRSRAWPH
                     RRVKLPLAAKIVSDLGRTDYMRVSITGGRVAPIMTTGASILSSTTRADGVVIVAAHHE
                     GHGEGEEVEVHLYDEDDSR"
BASE COUNT         1313 a         3002 c         3005 g         1173 t
ORIGIN      
        1 cgcgagcagc cgcgcgccga tctctcgcac cccgcgatcg cggtcaatct cgatgcctgc
       61 atccagtgca cgcgctgcgt gcgcgcgtgc cgcgaggagc aggtgaacga tgtcatcggg
      121 tacgcgttcc gcggcgagca ctcgaagatc gtgttcgatc tcgacgaccc gatgggcggg
      181 tcgacctgcg tcgcgtgcgg cgaatgcgtg caagtgtgcc cgaccggcgc gctcatgcct
      241 gcgcgtgacg tcgccctcgc gaaggcggac cggaccgtcg actcggtctg cccgtactgc
      301 ggcgtgggct gtctcctgac ctatcacatc cgggacaaca agatcctgca cgtgcagggc
      361 cgggacggtc cggcgaacca gagccggctc tgcgtgaagg gtcgctacgg gttcgactac
      421 gtccatcaca agcagcggct caccacgccg ctggtgcgca agcccggtgt gccgaagacc
      481 aaggatttca ccgttcatcc cgagcactgg cacgaggcat ttcgcgaggc gacctgggaa
      541 gaggcgctcg agcttgcggc ttccgggttg cgcgaactcc gcgaccgcca cggcaagcag
      601 gcgctcgccg gcttcgggtc cgcgaagtgc acgaacgagg aggcgtacct gttccagaag
      661 ctcgtgcgca ccggtttcgg ctcgaacaac gtcgaccact gcacgcggct ctgccacgcc
      721 tccagcgtcg cggcgctgat ggaaggcatc aactcgggcg cggtgtcgaa ccaggtgcgc
      781 gacgtggaga aggccgaggt cgtcatcgtc atcggcgcca acccgaccgg aaaccacccg
      841 gtcgcagcga cgtggatcaa gaacgccgcg aagcgcggaa cgaagctgat cgtcgccgat
      901 ccgcgccgca ccgatctcgc acgatacgcg acgcacgtgc tgcaattcca cgccgacacc
      961 gacgtcgcgc tgctcaatgc gatgctgcac acgatcgtcg aggaagggct cgtcgacgag
     1021 gcgttcgtcc gcgaccgcac ggccaacttc gaggccctgc gcgagaacgt gcagaagtat
     1081 tcgcccgagt cgatggcgcc gatctgcggc atcccggcgt cgacgatccg agaggtggcg
     1141 cggctctacg ccacgtcgaa ggggtcgatg atcctgtggg gcatgggcat ctcgcagcac
     1201 gtgcacggta ccgacaacgc gcgctgcctg atcgcgctgg ccctgtcgac cggccagatc
     1261 gggcggccgg gcaccggttt gcatccgctg cgcggccaga acaacgtgca gggcgcgtcc
     1321 gactcgggct tgatcccgat ggtgtatccc gactaccagc gcgtcgacaa cccggaggtc
     1381 aggagcagct tcgaacggct gtggggcaca gagctcgacc ccagaccggg gctgacggtg
     1441 gtcgagatca tcaacgcggc gaagcagcgc gagatccgcg gcatgtacat catgggtgag
     1501 aaccccgcga tgtcggatcc ggacctcgaa cacgcgcgcg aggcgatcgc cgcgctcgat
     1561 cacctggtcg tgcaggacat cttcctcagc gagaccgcgt atctcgccga cgtggtgctt
     1621 cccgcgacgg cgtggcccga gaaggtcggg acggtgacca acaccgaccg catggtgcag
     1681 ctcggtcgaa aggccatcga gccgccgggc gatgcgcgcg aggacttctg gatcatccag
     1741 gagctcgcaa ggcgcatggg cctgccctgg cgctacgagc acccgcgcga cgtattcgac
     1801 gagatgcgcc ggtgcatgcg ttcgattgcc gggatcacct gggaccggct cgagcgcgac
     1861 tcgtcggtga cctatccatg cgagaacgag ggcgacccag gcgagccggt cgtgttcgtc
     1921 acgcagtttc cgaccgcgac cgggcgcggc aagttcgtcc cggcggacat catccgcgcg
     1981 gcggagcagc ccgacgacga gtatccgatg gtgctcatca ccgggcgtca gctcgagcac
     2041 tggcataccg gctcgatgac gcgccgggcg ggcgtgctgg atgcgatcga gcccgagccg
     2101 gtggcgagct tccatcgcaa cgatctcgag cggctcggcg tgacacccgg cgaggtggtc
     2161 accgtcgaat cgcggcgggg cgcggtctcg ctctacgcgc gggtcgacga tggcacgcca
     2221 gccggagccg tgttcatgcc gttctgttac tacgaggcag ccgcgaacaa gctcaccaac
     2281 ccggtgctcg acccggtcgg gaagattccc gagttcaagt actgcgccgt gcgtgtcgcc
     2341 aggggcggcg agatcccgaa gcaggcgagc tacggcgggg gacagacgcc gctgcccggg
     2401 cagcggtgat gcagaaaacg cgcgatcgca aggtccggct ctgccggacc gcgcagcaac
     2461 gcgcagcgtt gcggaggtcg atcgcaaggt ccggctctgc cggaccgcgc agcaacgcgc
     2521 agcgttgcgg aggtcgcgat tgaacgtcga cgacggaaag gctacgcttc cgcggtggtc
     2581 accatcgagc tctacggcgt gccgcggatg cgcgcggggc gcgacgagat aaccgtcgag
     2641 gcaggctgcc tcggcgacgc gctggcggcg ctgggacgcg agtgcccggt gctggcgcca
     2701 tcggtggtcg acgggcaacg tctacagccg tgctacgtgg tggccatcaa cggcatccag
     2761 ttcactgcgg acgcgaagca ccctcttcgc gacggcgacg ccatcgttct gctgtccgcc
     2821 gacgccggag gctgacatgc gcggggggtt cttcggggag tacctccgga tcgacctcgg
     2881 ctcgggcgag gcgatccggg tcgcgctcga ccccgcgatc gcccgccgct tcatcggcgg
     2941 cgtcggtctc ggagcctggc tgctgcacca ggagaccccg cgcggcttcg atccgctcgg
     3001 agccgaggcg gccgtgatct tcgccttcgg gcccctcctg ggcacgccgc tcacgaccag
     3061 cgccaagtac gccctcgtcg ccaagtcgcc gctcaccggg cgcatcggag acgcgctctc
     3121 gtcgtcgcgc ttcgcggtcg cggccaagcg aatgggcgtc gacgcgatcg tcctcaccgg
     3181 gtgcgccgcc cgtccctcgg tgctgctcct cgacgaagac cgcgcgtcgc tcgaggccgc
     3241 cggcgacctc tggggccacg gcctgccggt ctccgaggca gcccgggcgc tcgcgacccg
     3301 gtttcccggg cacgcattcg cagggatcgg acccgccggc gagcacctcg tgcgctacgc
     3361 cggcgtcacc aacgaaggcc gccatgccgg aagaggcggg ctcggggccg tgctcggcgc
     3421 gaagcggctc aaagcgatcg gcgtgcgcgg cgtgcggcgc gtcccgctcg ccgacgccgc
     3481 gaagaccacc gcgctctcga aggcgctcgc caggcggtcg ctgggtccgg ccaccgagaa
     3541 gtaccgggag ctgggcacgg tcggaaacct tgcggcgttc aaccgtctcg ccgcgttgcc
     3601 gacgcgcaat ttccaggaga gcacattcga gggcgcggtc gacgtctcgg gcgagatcct
     3661 ccatgccacg cgtcgcaagg ggcgcggctc gtgcatcaac tgcaccatcg ggtgcgagca
     3721 cttcttcgag gtggcgcccg gcgaggcgcc ggtcaaggcg gaatacgaga acgtcttcgc
     3781 gctcggcccg ctgtgcggca tccgcgaccc cgaagtcgtc ctccgcgcgt cccgcagctg
     3841 cgacgagctg ggcctcgaca cgatctccgc cggcggcacc atcgcgttcg ccatggagtg
     3901 cgccgagcgc ggcctgttcg acggcacggt cttcgaggcc caggcgcgcg gactgcgctt
     3961 cggcgacggc gcgcggctgc tcgccctcct cgatgacatc gcgttccgcc gcggcgcgct
     4021 ggcggacctc ctcgcggagg gtagccgcag cgccgccgcg cggctcgggc cgccggcacc
     4081 cgacttcgca ccccacgtca aggggctcga gatcccgggc tacgagccgc gggccctcca
     4141 gagcatggcg ctcggcttca gcgtcgggag ccgcggcgcc gatcacaacc gcagcggagc
     4201 ctacgaggcc gacttttcgg gccaggtcga tcgcttccac gtctcggccg aatcggcgcg
     4261 gagtgcgatc gccaccgagg accgggccgc catgctcgac tcgctgatcc tctgcaagtt
     4321 cctccgcggg acgctggtcg acttcttcgg cgaatcggcc gagatgctgg cggccgtgac
     4381 cggcttcgat tgcgacgcgg acgagctgcg ccgcgccgcc cagcgcatcg ttctcctgaa
     4441 gcggctgttc aacgagcgcg agggctggac ctgtgcggag gacaccctgc ccgagcggtt
     4501 cttcacccag tccctggcca aaggcgccgc cagcggcgcc gtgatctcgc gcgcagggct
     4561 ggcccattcc atcgccgcgt actacgaagc ccgtggcctc cgtcccgacg gaacggttcc
     4621 cgccgagacc ctgtgcgcca accagctcga tacgctgtag gctatcattc cggatccggt
     4681 ccgggcccga cgcgggcccc gagcgattgc aaggcgcgct cgtagtcatc gggcgtgttg
     4741 atgttctcga gcgatcgcag cccgggatcc gcggtcgcga ggtcggggtc ggcgagcaac
     4801 gcaggggcgt cgcagaggac ggtgcggacc cgctcgaaca ggaaaaacgg gcgaagccgg
     4861 tcggcggcga gcaaggcctt gatgtgggcg agacacgtgc acgcgtagat cgccgtgagg
     4921 gggtgaaagt agtcgcccat gttcggcacc gcgatgtcgt ggtcgccgcc tcggagcgcg
     4981 gcgagccggg cgatcagcgc cggctcgagg aacggcgcgt cggtggtcga cacgaatgcg
     5041 gcgtcgaccg cttcgctgcc ccccggcgac tcgagcagcg cctgcagccc ggccgcgatg
     5101 ccctggagcg gcccgcgccc ttcgaccgga tcgcgcgcga tggcgacgtc cggcggcaac
     5161 ggcggaacgt cctgtccggg cgcggcgacg acgatgacgg gagcacaggc gcggcgtacc
     5221 gtgcgcacga cgcgggcgag gagcggctcg ccgccgaaat cgagccacgc cttgggcgat
     5281 cccatccgcg agctccgccc gccgcagagc acgatcccgc cgaggcgcgg caccgggagc
     5341 atcacgcgac cagcccgcgg gcgcggaggg cggcacgcgt cgccggtcgc tccagctcct
     5401 cgatgaaagc gcgcaccgcg gcacgatccc tccgcgcccg ccgcacgaag aaatcgtagc
     5461 gctcctccgc gatgggcgcg aacccgagcc cgcgctcgcg ggcgacgaca tcgatggcga
     5521 cgccgaagtc ggcgcgagcc tgggcgacgg ccgccgcgat ggcgtggtgc gatgaggcct
     5581 cgacgccgtg gccgtcgggc cggagatctc cgagcagccg atcgatgatc gcacgggtgc
     5641 cgctgccgcg gttgcggttg accatcacca ggccgggcgt cgcggcggcc tcgcggaatg
     5701 ctacctccgc gggcttgcct tcgaaccgct cgtcgcccgg gcggaacacg acgccctgga
     5761 gccggccgta gccgcgcacg aggtcgagat cgggggtgag gaacggttgg ttgtacgcac
     5821 cggtcgctgt gtcgaacaga tgggcgccgg cgacatcgca ctcgccgcgc tgcacggcgc
     5881 gaaggccgcc ttcgctgccg atggcgatga gcttgacggt gagcccgcgg gcgcgaaggc
     5941 gcgccaggat gatgtcgagc cccacgcagt ggctgccgat gacgaccagg tcgtccttgc
     6001 gagcgcgggc gccggcggtc gcgagcacgc tcacctcttc gcgcgcgtcg agctgctcga
     6061 cggccctggg gatcacgaag tagccgtcgg cctggctcca cgccgtcacc gagccggagc
     6121 ccttgccgat cggatatgcg accagcgagc cggcgtcgtc ctcgacgagg cgcaccagca
     6181 cgtactcggt gcggccgacc tccgagctga tcgcgtacgg cactcgcgcg gcgacgcgag
     6241 ccacatcctg ctcgtcgcgc cccgcgagct tgcggatgac cggcgcgacg aattcgtgga
     6301 acgtgaagat ggcgctcgcc ggaaagccgg ggagcacgac gaccggcttg ccgccggaga
     6361 ccgcgaggca gatcggcttc cccggcttga gcgcgactcc gtggaccacg atgccggacg
     6421 gcccgaggat ggcttcgagc gcgcgcacgt tgagatcgcc cgggcccttg gacgtgccgc
     6481 cggacagcag gacgacctcg gcggccgcca ggccgcgcgc gagcgccgcc ttcacggctt
     6541 cgaggtcatc gccgacgacg ccgagcggaa tgaccgttcc gccctgctcg cgcaccgcgt
     6601 cgccgaggat ggtcgcgttg gagtcgtaga tcttcccgac cgcgatgggc gcgcccggcg
     6661 cgacgatctc gtcgccggtg gagaggatcg ccacccgagg ctgcgcgacg accgagacgc
     6721 gcgcgatccc catcgccgcg aggacgccgg tctcccgtga ggtgagcacg tcgcgcgcgc
     6781 gcagcacggt ctcgccttgc gcgacgtcgg tcccggcgaa actgatgttg cgaccgggcg
     6841 tgagcgcctt cgtcaccacg agcgcgtcat cctccatcga ggtgtgctcg accatcaaga
     6901 ccgcgtcggc tccgcgggga acgacgccgc cggtagggat cgccatcgcg ctgcccggtg
     6961 cgacggcggc gctcggcacg gcccccatcg cgacccgcgc tgcgagcagc cgcagcttct
     7021 tcggcgcgaa ctcgctggcg ccgaaggtgt cttgcgcccg gactgcgaac ccgtccacgt
     7081 tggcgcgatc gaagccgggc acgtcgaccg ggctgcagac gtcctctgcg aggactcgtc
     7141 cgagcgcctc gtggagagcg accacctccg tgcgccgcgg cgaaaggtca ccgagcgctg
     7201 cgcggaacga tctctctgcc tcgtcgcgat cgacgacgtg gagaaactgt tcctgcttca
     7261 tcgcgaatca tcctcgtcgt agagatggac ctcgacctcc tcgccctcgc catggccctc
     7321 gtgatgggcg gcgacgatga ccacgccatc ggcgcgcgtc gtggacgaca ggatcgacgc
     7381 gccagtggtc atgatcggcg caacccggcc gccggtgatc gagacccgca tgtaatcggt
     7441 gcggccgaga tcggagacga tcttggccgc gagcgggagc ttgacgcggc ggtgcggcca
     7501 cgcgcgggac cggccaccga gggcgcggat ggtcggtccg gcaaagaact cgtacgcgca
     7561 caggcacgag accgggttgc cgggaagcaa gaacaccggg cagccactgc gaagaaagcc
     7621 gaagccggcg gggctcgcgg gtcgcaaggc aaccccgtgg accgcgacct cgccaagctc
     7681 ggccagcacc gtcggggcgt ggtcctcggg tccgaccgac gacccgccgg agacgaggag
     7741 gacgtcaccg tcgtgggccg caaacagcgc gcgcagcgcg tcgcggtcgt cgggcaggta
     7801 gcgaagctcg atcgcgccgg cgccgtcgcg ctcggcgagc gcacggagca tcggaccgtc
     7861 ggcgtccgag atcagcgcgt cgcgcggcat gtctccggcc gggagcactt cgtcgccggt
     7921 gatcacgatc gacactcgcg gcctgcgcac cagcaggatc tcggccaccc cgaggctggc
     7981 gaggacgccg acgtcctgcg gccgcaacct tcgcccgcgc tccacgacca ggtcgccgcg
     8041 ccggatgtcc tcaccgaccg tgctgacgtg cttgccggga gcgaccgcct cgcggacgcg
     8101 gaccgtatcg ccagttgcct cggcgatctc ggccatcacg atcgcgtcgg cgccggtggg
     8161 gaccggcgca ccggtcgtga tccgcaccgc ctcaccggtg cgcagcgtcc cgccgaacca
     8221 tcgccccgcc ttcgtctctc cgatgagcgc gagctcgacc ggcgcctggg ccgatgcgcc
     8281 gaacgttgac tcgccgacga ccgcgaagcc gtccatcgcg ctccgagcga aggacggcac
     8341 gtcgacgccg gcgatcacgg cctcggctgc gacacgaccg tgcgcagcgc tgacgccgac
     8401 gcgctcgacg ccgaggccaa gcgaagccgt gcggcgctcg aggatcgcga tcgcagccga
     8461 gacgctcgtt ctctcgcgaa atccgtgcat gcg
//
LOCUS       DASVOR010001787         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_48017,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001787 DASVOR010000000
VERSION     DASVOR010001787.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48017"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VF516_33275"
     CDS             <1..218
                     /locus_tag="VF516_33275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112666.1"
                     /translation="SQVASPPGHCQAAQQGSSRSIGLARADQWQMPAPTWARAIGAIL
                     DTASMADERGGRPAAVNQGTWLAVQDA"
     gene            complement(679..972)
                     /locus_tag="VF516_33280"
     CDS             complement(679..972)
                     /locus_tag="VF516_33280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112667.1"
                     /translation="MTSASGARFHADGRGHRRSASKISRSSGTAGPSAASADAIAASA
                     IAAFQPMAGICHWSALARPIEREPPRLSGSIRPDNDLVDLQPDSETRSRMKPI"
     gene            complement(969..1436)
                     /locus_tag="VF516_33285"
     CDS             complement(969..1436)
                     /locus_tag="VF516_33285"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8112668.1"
                     /translation="MTTRLATAADHETVVACVRAAYTPYIAEIGVAPAPLQDDYAEHI
                     ARGRVWLAVCSNDEVAGLMVILIEDEHLLIDNYAVRPEWQNRGISKLLTTIAEQAARR
                     HGLGKLRLYTNAKMTKNLALYRRRGWVEVERRTEHGFDRVYMERSLPPDEGWP"
     gene            complement(1575..>1794)
                     /locus_tag="VF516_33290"
     CDS             complement(1575..>1794)
                     /locus_tag="VF516_33290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112669.1"
                     /translation="VPCHSSHPTDPAFPAPPNNVVFETADQIKLMVPRIRERAVESKT
                     MPFLNRTQITPVERAELGKWITDGAHLQ"
BASE COUNT          291 a          585 c          606 g          312 t
ORIGIN      
        1 ggagtcaggt cgcaagtcca ccaggtcatt gtcaggccgc tcagcagggc agctcgcgct
       61 cgatcggcct ggcgagagcg gaccagtggc aaatgccggc acccacgtgg gcgcgcgcca
      121 tcggtgccat cttggacacc gcatcgatgg ccgacgagcg cgggggaagg ccagcggccg
      181 tgaaccaggg cacctggctg gcggtgcagg acgcgtgacc gcgccgtgac tcgtgtggac
      241 agccgcgtcc aacgtcgagg ccgacatggc gcgcgcctgc cgcacacgac tcaccgcgct
      301 gacgccggca tgcgcgctcc acgatcggtt gttcttgatc cggttccgga gtcaggtcgc
      361 aagcccacca ggtcattgtc aggcctgaca gagccgctca gccgcggcgg ctcgcactcg
      421 atcgggctgg cgagagcgga ccagtggcaa ataccggcac ccacgtgggc gcgcgccatc
      481 ggtgccatct tggacaacgc atcgatggcc gacgagtgcg ggggaaggcc agcggccgtg
      541 aaccagggca cccggcaggc tgtgcaggac gcgtgaccgc gccgtgactc gtgtggacag
      601 ccgcgcccga cggtcgaggc cgacatggcg cgcgcctgcc tcacacgact caccgcgctg
      661 acgcctgcat atgcactcct agatcggttt cattcttgat ctggtttcgg agtcaggttg
      721 caagtccacc aggtcattgt caggcctgat agagccgctc agccgcggcg gctcgcgctc
      781 gatcggcctg gcgagagcgg accagtggca aatgccggcc atgggctgga aggcggcgat
      841 tgcagaggcg gcgatcgcgt ccgcgctggc ggcgctgggt ccggcggtac cgctcgaacg
      901 gctgatcttc gaggcgctgc gtcgatgccc gcgaccatcc gcatgaaagc gcgcgccgct
      961 ggccgatgtc atggccagcc ttcgtcgggt ggcagcgatc gctccatgta gacgcgatcg
     1021 aagccgtgct ccgtgcgtcg ctcgacctcg acccatccgc ggcgccgata cagcgcgagg
     1081 ttcttcgtca tcttcgcgtt ggtgtagagc cgaagctttc cgaggccatg gcgccgggct
     1141 gcttgctcgg cgatcgtggt cagcagcttg ctgataccgc ggttttgcca ttccggtcgg
     1201 acggcatagt tgtcgatcaa caggtgttca tcttcgatca agatcaccat caagcccgca
     1261 acctcgtcgt tgctacagac tgccagccag acccgccccc gcgcgatatg ctcggcgtag
     1321 tcgtcctgca atggtgccgg agcaacgccg atttccgcga tgtacggtgt gtacgcggcc
     1381 ctcacacaag ccacaacagt ctcgtgatcg gccgcggttg ccagacgcgt ggtcacgtgc
     1441 gtcggttcca gatctgaact caccgtacct cggacacagc aatggttttc atactgcgag
     1501 gacaggcgaa ggttcaatgc tttcgatccg tcccggcgct ccggagcgat cgggacagcg
     1561 cccacgtggg cgcgctactg caggtgagcg ccgtcggtga tccacttgcc gagctcggcg
     1621 cgctccaccg gggtgatctg ggtcctgttg agaaacggca tggtcttgct ctcgaccgcg
     1681 cgctcgcgga tccgcggcac catcagcttg atctggtcgg cggtctcgaa cacgacgttg
     1741 ttgggcggcg ccgggaacgc cggatccgtc gggtggctgc tgtggcaggg cacg
//
LOCUS       DASVOR010001788         2095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_486243,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001788 DASVOR010000000
VERSION     DASVOR010001788.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486243"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VF516_33295"
     CDS             complement(<1..508)
                     /locus_tag="VF516_33295"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8112670.1"
                     /translation="MGQQLAIVLIGVGGILVLGVLVLIARTWRQVDQGYAMIVNKMGK
                     EPKVTFTGAIVLPIVNRAEIMDLSVKTIHVARRGKDGLICMDNIRADINVTFFVRVNK
                     TADDVLRVAQQIGCARASQQSTLEELFAAKFSEALKTVGKQMEFEQLYTQRNVFKDRI
                     IEVIGTDLH"
     gene            complement(599..1345)
                     /locus_tag="VF516_33300"
     CDS             complement(599..1345)
                     /locus_tag="VF516_33300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112671.1"
                     /translation="MNELVVASLQFPTVVFTIALGIVLVYWLFVLIGALDIDLFGGDV
                     DVDVSGAAKGIGDVLTGGAKGGAEALHGGGHAGGEAGGDADGDLDGGGLWHVLGLGDV
                     PVTISVSLITVLAWVGSLLATHTLGIGGWLTLVVLVVAVAIALPIAALLIRPIAPVFA
                     VKEGKSNADYIGHVCTITTGRVDNSFGQATIEDGGTVLDIAVRCDRPGTLARGDKALI
                     IEFDRERQAYIVEPSGDVLAGGGARGGEST"
     gene            1652..>2095
                     /locus_tag="VF516_33305"
     CDS             1652..>2095
                     /locus_tag="VF516_33305"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HEX8112672.1"
                     /translation="MGEASSDLRPGPGRSDLVGLLPIALGLLAAEDGQYASLKADLRR
                     WAKEDPIDALVATVLGGGIAFYLAERDTNPGCATPWDAILYMATCLSVGYDNLFPTTP
                     TGHMLASLVQTFGPALSGMAFDAPAAEARAEAEAARAEARAQAEEA"
BASE COUNT          341 a          777 c          655 g          322 t
ORIGIN      
        1 cgtgcaggtc ggtgccgatc acctcgatga tccggtcctt gaacacgttg cgctgggtgt
       61 acagctgctc gaactccatc tgcttgccga ccgtcttgag cgcctcggag aacttggcgg
      121 cgaacagctc ctcgagcgtc gactgctgcg acgcgcgggc acagccgatc tgctgcgcga
      181 cccgcagcac gtcgtcggcg gtcttgttga cccgcacgaa gaacgtgacg ttgatgtcgg
      241 cgcggatgtt gtccatgcag atcaggccgt ccttgccgcg ccgcgcgacg tggatcgtct
      301 tgaccgacag gtccatgatc tccgcgcggt tgacgatcgg cagcacgatc gcgccggtga
      361 acgtcacctt gggctccttg cccatcttgt tgacgatcat cgcgtagccc tgatcgacct
      421 gccgccacgt ccgtgcgatc agcacgagga ccccgagcac caggatgccg cccacgccga
      481 tgagaacgat tgccagttgc tgacccatgt gaactcctcg gccggtcccc cgaccggccc
      541 gctacgcagc gttgccatcc atcaaatcag gccaagcggc caagatacaa gttcgacgct
      601 aggtcgactc gcccccgcgc gcaccgccgc ccgcgagcac gtcccccgac ggctccacga
      661 tatacgcctg acgctcgcga tcgaattcga tgatcagcgc cttgtcaccg cgtgccagcg
      721 tgcccggccg gtcgcagcgc accgcgatgt ccagcaccgt gccgccgtcc tcgatcgtcg
      781 cctggccgaa gctgttgtcg acccggccgg tcgtgatcgt gcagacgtgg ccgatgtagt
      841 cggcattcga cttgccctcc ttgaccgcga acaccggcgc gatcggccgg atcagcagcg
      901 cggcgatcgg cagcgcgatc gccaccgcga ccaccagcac caccagcgtc agccagccgc
      961 cgatcccgag ggtgtgcgtc gcgagcagcg agccgaccca cgccagcacg gtgatcagcg
     1021 acaccgagat cgtcaccggg acgtcaccga gcccgagcac gtgccacagc ccgccgccgt
     1081 cgaggtcgcc atccgcgtcc ccgccggcct cgccgcccgc gtgaccgcct ccgtgcagcg
     1141 cctcggcacc gcccttggcg ccgccggtca gcacatcgcc gatgcccttc gccgcgcccg
     1201 acacgtcgac gtcgacatcg ccgccgaaca ggtcgatgtc gagcgcgccg atcagcacga
     1261 acagccagta cacgagcacg atgccgagcg cgatcgtgaa cacgaccgtc ggaaactgca
     1321 gcgacgcaac cacgagctcg ttcaactcga cctccggggg cggtgtgttt cttcacgtgt
     1381 aagtcgcccg gactcaccgc ggaagacgtg cgcgaccgac aatcggacac aacgcgctgc
     1441 gtttgcaacc cggtccagga tgacattgga atgcgcgccg cgcccggttc gttgcagcaa
     1501 accgcgaagc tggcgcccgc gctggagatc caatcaaacg gatctctcga tgggaatcat
     1561 tgccccacgt gcggcgtaac atcggatcgc gcccgtcgcg gccggccgcg ttgcttgcca
     1621 cccggtcgcc gggtgtctac gttgtatgca catgggggaa gcttccagcg acttgcggcc
     1681 tgggcccggg cgcagtgatc tggtcggcct gttgccgatc gcgctcggcc tgctcgccgc
     1741 cgaggatggc cagtacgcgt cgctcaaggc cgacctccgg cgctgggcca aggaagaccc
     1801 gatcgacgcc ctggtcgcga ccgtgctcgg cggcggcatc gcgttctacc tcgccgagcg
     1861 cgacaccaac ccgggctgcg cgaccccgtg ggacgcgatc ctctacatgg cgacctgcct
     1921 gtcggtcggc tacgacaacc tgtttccgac cacgccgacc ggccacatgc tcgcgtcgct
     1981 ggtccagacc ttcggccccg cgctgtcggg catggcgttc gacgcgccgg ccgccgaggc
     2041 ccgcgccgag gccgaggccg cgcgcgccga ggcccgagca caggcggaag aggcc
//
LOCUS       DASVOR010001789         2144 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_544699,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001789 DASVOR010000000
VERSION     DASVOR010001789.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544699"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /locus_tag="VF516_33310"
     CDS             complement(<1..1305)
                     /locus_tag="VF516_33310"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112673.1"
                     /translation="MRQPVPFGKYLLLDRISVGGMAEVFKAKSYGVEGFEKIIAIKRI
                     LPTMGEDRDFIKMFIDEAKIAGQLAHANICQIFELGRIDGSHFIAMEYIWGKDLLQIQ
                     NRLRKLTQPMPIPMACFTIAKVLEGLDYAHRKRDPLGRPLEIVHRDCSPQNVLVSYEG
                     EVKVIDFGIAKATSRNSRTMAGVLKGKFGYMSPEQVRGLPLDRRSDIFALGTMLYECL
                     TSDRLFQGETDFSTLEKVRNVDVRPPREINPNIPPAVEAVIMKALAKDVEERYQWCSE
                     MLADLQAFLMSQDVVFTAKSLSSWLKEVFAAEIDRERQQLEQYKRVGRDGLIDGMPAA
                     EVKADVVANLGEAPPVEGDPTVLGGPNFDDIEQAAGASPEVGPAPGSPATRATSESDE
                     SEDFGEEAPTEIFGEIDHGANPALAAAAAMAGAGRPPGASQPP"
     gene            complement(1302..>2144)
                     /locus_tag="VF516_33315"
     CDS             complement(1302..>2144)
                     /locus_tag="VF516_33315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112674.1"
                     /translation="LAGDWPRVAELVDPLLREQLPIADLAEAHRLAGIAAFFAQRDAA
                     AEQHFVTYLRVDPDGRLDPALYPPEVVAFFNDVASRHAAELRALRVPPQRPSWLFTLI
                     PPAGQYVNGEHTKAYVLGGVLGALLVTNLVTYSYLRQWCDHTQGSAGGQLTCTDGDQP
                     GHDATQAARRLRPFNIASGIAFWVVYAFGVYDGIRGYRRISREQALRPYVTASSDSGV
                     VGISGRFWSAFETPTWWLSPIDHGLVAPCAQRHSAGVVADPRSDAPLSPLCSFWTVRY
                     HLTW"
BASE COUNT          335 a          736 c          736 g          337 t
ORIGIN      
        1 cggcggctgc gacgcgcccg gcggccgccc tgccccggcc atcgccgcgg ccgcggccag
       61 cgcggggttc gcgccgtgat cgatctcgcc gaagatctcg gtcggcgcct cctcgccgaa
      121 gtcctcgctc tcgtcgctct ccgaggtggc gcgggtcgcc ggcgagccgg gcgccggacc
      181 gacctccggg ctcgccccgg cggcctgctc gatgtcgtcg aagttcgggc cgccgagcac
      241 cgtcggatcg ccctcgaccg gcggcgcctc gccaaggttc gcgacgacgt cggccttgac
      301 ctcggcggcc ggcatgccgt cgatcagccc gtcgcggccg acccgcttgt actgctcgag
      361 ctgctggcgc tcgcggtcga tctcggcggc gaacacctcc ttgagccagc tcgacagcga
      421 cttggccgtg aacacgacgt cctggctcat caggaacgcc tgcaggtcgg cgagcatctc
      481 gctgcaccac tggtagcgct cctcgacgtc cttcgcgagc gccttcatga tcaccgcctc
      541 gaccgccggc gggatgttcg ggttgatctc gcgcggcggc cggacgtcga cgttgcggac
      601 cttctcgagc gtcgagaagt cggtctcgcc ctggaacagc cggtcgctgg tcaggcactc
      661 gtagagcatc gtgccgagcg cgaagatgtc gctgcggcga tcgagcggca ggccgcggac
      721 ctgctccggc gacatgtacc cgaacttgcc cttgagcacg ccggccatcg tccgcgagtt
      781 gcgcgacgtc gccttcgcga tgccgaagtc gatgaccttg acctcgccct cgtacgacac
      841 cagcacgttc tgcggcgagc agtcgcggtg gacgatctcc agcggccggc cgagcgggtc
      901 gcgcttgcgg tgcgcatagt ccaggccctc cagcaccttg gcgatggtga agcacgccat
      961 cgggatcggc atcggctgcg tgagcttgcg cagccggttc tggatctgga gcaggtcctt
     1021 gccccagatg tactccatcg cgatgaagtg cgagccgtcg atccggccga gctcgaagat
     1081 ctggcagatg ttggcgtgcg ccagctgacc ggcgatcttc gcctcgtcga tgaacatctt
     1141 gatgaagtcg cggtcctcgc ccatcgtcgg caagatccgc ttgatcgcga tgatcttctc
     1201 gaaaccctcc acgccgtacg acttggcctt gaacacctcg gccatgccgc ccacgctgat
     1261 gcgatcgagc agcaggtact tcccgaaagg tacgggctgc ctcaccaggt cagatggtag
     1321 cggacggtcc agaacgagca caatggcgaa aggggggcat ctgaccgggg gtcagcgaca
     1381 acacccgcag agtgtcgctg agcacacggg gccacaagac catggtctat cggcgacagc
     1441 caccatgtag gtgtctcgaa agcgctccaa aatcgaccgg agatcccgac gacgccgctg
     1501 tcgctcgacg cggtgacgta ggggcgaagc gcctgctcgc gtgagattcg ccgatatcca
     1561 cgtataccgt cgtacacgcc gaatgcatat accacccaga aagcgatccc gctcgcgata
     1621 ttgaacggcc ggagccggcg ggcggcctgg gtcgcgtcgt gtccgggttg atcgccgtcg
     1681 gtgcaggtca gctggccgcc ggcggacccc tgggtgtgat cgcaccactg gcggagatac
     1741 gagtaggtca ccaggttggt gaccaggagc gcgccgagca cgcccccgag cacgtaggcc
     1801 ttggtgtgct cgccgttcac gtactggccg gccggcggga tcagcgtgaa cagccacgac
     1861 ggccgctgcg gcgggacgcg cagcgcgcgg agctcggcgg cgtggcgcga cgcgacgtcg
     1921 ttgaagaacg cgacgacctc gggcgggtac agcgccggat cgagccggcc gtcgggatcg
     1981 acccgcaggt acgtgacgaa gtgctgctcg gcggccgcgt cgcgctgcgc gaagaacgcc
     2041 gcgatcccgg ccaggcgatg cgcctcggcg aggtcggcga tcggcagctg ctcccggagc
     2101 agcggatcga cgagctcggc gacgcgcggc cagtcgccgg cgag
//
LOCUS       DASVOR010001790         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_76891,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001790 DASVOR010000000
VERSION     DASVOR010001790.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76891"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VF516_33320"
     CDS             <1..580
                     /locus_tag="VF516_33320"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEX8112675.1"
                     /translation="RTRYQGDRGQPVPVDASAALAGAAALLIDGTAPADQLHAAELAK
                     QTKQPVIFDVGELRDGTVELIRVTDILIASEREASELAPRGELTDALDELLALGPRAV
                     VITLGDKGAIGRHGEQIVRCPAFPGEVLDAYGAGSVFHGAFAAALLSELPFARCMELA
                     AAAASLSLRELGPWSAMPSREDVLALVRTRRT"
     gene            777..1352
                     /locus_tag="VF516_33325"
     CDS             777..1352
                     /locus_tag="VF516_33325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112676.1"
                     /translation="MHRLFAPAALSAVFLVACASSASAEPSAPATPKPTPAAPSAPAA
                     PATPAAPAAPATPADCKPTGRALFEIDHKVESGAKLATSAVKLYASGAWTHDETDAEG
                     KAQPQTAGCLARPEVKQVADALHAAPWKITTARIRCMAMSPQFTEYRVDGKLVFTQKL
                     CSGQSLDDKSRAALDKAIALVEPPGAPKPAP"
     gene            complement(1365..2078)
                     /gene="rnc"
                     /locus_tag="VF516_33330"
     CDS             complement(1365..2078)
                     /gene="rnc"
                     /locus_tag="VF516_33330"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEX8112677.1"
                     /translation="MADYSSLEAMLGYEFRDRSLLEQALRHASWCNEHPAERLEDNER
                     LEFLGDAVLDLVVGHKLMTRYPQLREGELSVTRAQVVSEAGLSEVAGQLGIGAWLMLG
                     KGEEKSGGRSKPSILADAFEAILAAVYLDGGFDAATGIVERLLAQRIETVEFKGFYDF
                     KTRLQETAQARLKATPTYRVVQEIGPDHDKRFVVAVTIGSDEWARAVGKSKKEAEQMA
                     AAEASFRLEGSNVGRPRKR"
     gene            2101..>2851
                     /locus_tag="VF516_33335"
     CDS             2101..>2851
                     /locus_tag="VF516_33335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112678.1"
                     /translation="MEALRERFGKYHVLEKIAQGGMAEVYKVKTVGIAGFEKIQALKR
                     ILPHQAREGRFIRSFIDEARIAVELTHRNIVQVFDFGKADGELFLAMELIEGKDLRTV
                     MTEAVARVALPEVAIAAYIISEVASGLDYAHRKADGYGSPLGIVHCDVSPSNVMLSSE
                     GDIKILDFGIARATFASALERRRLRGKPRYMSPEQTTGETPSAASDVFALGIIAWELL
                     TGLPLYRGTDLKAILEAVRRTVPQRIDRLNPK"
BASE COUNT          403 a         1060 c          990 g          398 t
ORIGIN      
        1 gcgcacccgc taccagggcg accgcggtca gccggtcccg gtcgacgcca gcgcggcgct
       61 cgccggcgcc gcggcgctgc tgatcgacgg caccgcgccc gccgatcagc tgcacgccgc
      121 cgagctcgcc aagcagacca agcagccggt gatcttcgac gtcggcgagc tgcgcgacgg
      181 cacggtcgag ctgatccggg tcaccgacat cctgatcgcc tcggagcgcg aggccagcga
      241 gctcgcgccg cgcggcgagc tgaccgacgc gctcgacgag ctgctcgccc tggggccgcg
      301 cgccgtggtg atcacgctcg gagacaaggg cgcgatcggc cgccacggcg agcagatcgt
      361 gcgctgcccg gcgttccccg gcgaggtgct cgacgcctac ggcgccggct cggtgttcca
      421 cggcgcgttc gccgccgcgc tgctcagcga gctgccgttc gcgcgctgca tggagctcgc
      481 cgccgcggcc gcgtcgctgt cgctgcgcga gctcggcccg tggtcggcga tgccgagccg
      541 cgaagacgtg ctcgccctgg tccggacccg gcggacctga tcgcggtgat ccgcggcaca
      601 ccgcgggagc cggcttgaat cccgcgcgca gccggtgcgt cgaaccgggc atgacacgcg
      661 aaccactccg gtcgatcccc gtctccggtc ctgccggcag cgctgctgcg tcgagccgtg
      721 catctggccc tgcctccggc catgcatccg gccgccgcgt gaggcatggt cggggcatgc
      781 atcgcctgtt cgcgcccgcc gcgctgtccg cggtcttcct ggtcgcctgc gcctccagcg
      841 cctcggccga accctccgcc ccggccaccc ccaagccgac gcccgccgcc ccgtcggcgc
      901 ccgccgcacc ggcgacgccc gctgcaccgg cggcgcctgc gacgccggcc gattgcaagc
      961 cgaccggccg cgcgctgttc gagatcgacc acaaggtcga gtccggcgcc aagctcgcga
     1021 cgtcggcggt caagctctac gccagcggcg cgtggacgca cgacgagacc gacgccgagg
     1081 gcaaggcgca gccgcagacc gcgggctgcc tcgccaggcc cgaggtcaag caggtcgccg
     1141 acgcgctgca cgccgcgccg tggaagatca cgaccgcgcg gatccgctgc atggcgatgt
     1201 cgccgcagtt caccgagtac cgggtcgacg gcaagctggt gttcacccag aagctgtgca
     1261 gcggccagag cctcgacgac aagagccgcg ccgcgctcga caaggcgatc gcgctggtcg
     1321 agccgcccgg cgctcccaag ccggcgccgt gacctcgcgg cggctcagcg cttgcgcggc
     1381 cgcccgacgt tgctgccctc gagccggaag ctggcctcgg ccgccgccat ctgctcggcc
     1441 tccttcttcg acttgccgac cgcgcgcgcc cactcgtcgc tgccgatcgt gaccgcgacg
     1501 acgaaccgct tgtcgtgatc ggggccgatc tcctggacga cgcggtaggt cggcgtcgcc
     1561 ttgagccggg cctgcgcggt ctcctgcagc cgggtcttga agtcgtagaa gcccttgaac
     1621 tcgacggtct cgatccgctg ggcgagcagg cgctcgacga tgccggtcgc ggcgtcgaac
     1681 ccgccgtcga ggtagaccgc cgcgaggatc gcctcgaacg cgtcggcgag gatcgacggc
     1741 ttggatcgtc cgcccgactt ctcctcgccc ttgccgagca tcagccacgc gccgatgccg
     1801 agctggccgg cgacctcgct cagcccggcc tcgctgacga cctgggcgcg ggtcacgctg
     1861 agctcgccct cgcggagctg cgggtagcgc gtcatcagct tgtgaccgac cacgaggtcg
     1921 agcacggcgt cgccgaggaa ctccagccgc tcgttgtcct ccaggcgctc ggcggggtgc
     1981 tcgttgcacc agctcgcgtg gcgcaatgcc tgctcgagca ggctgcgatc acgaaactcg
     2041 tagccgagca tcgcttcgag ggaggagtag tcggccacga cccatggtac cctcggatca
     2101 gtggaagcgc tgcgcgagcg tttcggcaaa taccacgtgc tcgagaagat cgcgcagggc
     2161 ggcatggccg aggtctacaa ggtcaagacc gtcgggatcg ccgggtttga aaagatccag
     2221 gcgctcaagc gcattctgcc ccaccaggcg cgcgaggggc ggttcatccg cagcttcatc
     2281 gacgaggcgc ggatcgccgt cgagctgacc caccgcaaca tcgtccaggt gttcgacttc
     2341 ggcaaggccg acggcgagct gttcctcgcg atggagctga tcgagggcaa ggacctgcgg
     2401 accgtgatga ccgaggcggt ggcgcgcgtc gcgctgcccg aggtcgcgat cgcggcgtac
     2461 atcatctccg aggtcgcgtc ggggctcgac tacgcgcacc gcaaggccga cggctacggc
     2521 agcccgctcg gcatcgtgca ctgcgacgtg tcgccgtcga acgtgatgct gtcgagcgag
     2581 ggcgatatca agatcctcga cttcggtatc gcgcgcgcga cgttcgcgtc cgcgctcgag
     2641 cgccgccggc tgcgcggcaa gccacgctac atgtcgccgg agcagaccac cggcgagacc
     2701 ccgagcgcgg cgtccgacgt gttcgcgctc ggcatcatcg cgtgggagct gctgaccggg
     2761 ctgccgctgt atcgcggcac ggacctcaag gcgatcctcg aggcggtgcg ccgcacggtg
     2821 ccgcagcgca tcgatcgcct gaaccccaag g
//
LOCUS       DASVOR010001791         1881 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_456681,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001791 DASVOR010000000
VERSION     DASVOR010001791.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1881)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1881)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1881
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456681"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="VF516_33340"
     CDS             complement(<1..637)
                     /locus_tag="VF516_33340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112679.1"
                     /translation="MAKKQSDGEAAEAAGKRRPQKKTAAPKPGARSKPAKGGKPSKPA
                     KPSKPSRSGRSGTTARMPRPTRPPPEPAQAELPIGDVDVIEDADILSETRVDAAAEAA
                     EARPARGEPEPAETDDVLEEAEGPAPEADEEDEDTEAGPADEVLEEVVEVEDQPPALS
                     PEEQELATMYGDELAQPAIAHAEYTDRQTADEDRPMMPEINARDERKLQWQE"
     gene            831..1871
                     /locus_tag="VF516_33345"
     CDS             831..1871
                     /locus_tag="VF516_33345"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX8112680.1"
                     /translation="MQSRKIGVLLGGMSSEREVSLRTGEAVLAALRERGHRAIPIYVD
                     RDVDVALRQEQIEVAFIALHGRWGEDGCIQGLLEMLGIPYTGSDVLASALAMHKVKAK
                     ELFRLHNLPTPAYYTLTGAQLADGPEETPADALAAIHGDFGFPCVVKPVREGSSVGVS
                     ICRDMAELGPAALRALRFDDEILVERFIAGREISVAVLGDRAIGAVEIAPRDGFYDYA
                     NKYIRGATDYYVPPRVSPERYRGLLTQALRAHLALGCSGVTRVDLMLSEAGNEFILEV
                     NTLPGLTPTSLLPKIADAAGISFGELCEMMLSGASLATRRGRGERRIAQRTFVGDDRR
                     QGDAVAERYDHH"
BASE COUNT          226 a          681 c          663 g          311 t
ORIGIN      
        1 gctcctgcca ctgcagcttg cgctcgtcgc gcgcgttgat ctccggcatc atcggccggt
       61 cctcgtcggc ggtctgccgg tcggtgtact cggcgtgcgc gatcgcgggc tgggcgagct
      121 cgtcgccgta catcgtggcg agctcctgct cctcggggct gagcgccggc ggctgatcct
      181 cgacctcgac cacctcctcc aggacctcgt cggccggtcc ggcctcggtg tcctcgtctt
      241 cctcgtcggc ttctggcgcc ggcccctcgg cctcctccag cacgtcgtcg gtttcggccg
      301 gctcgggctc gccacgcgcc ggtcgggcct cggcggcctc cgccgcagcg tcgacccgcg
      361 tctcggacaa gatgtcggcg tcctcgatga cgtcgacgtc gccgatcggc agctcggcct
      421 gggcgggctc ggggggcggc cgggtcggtc ggggcatccg ggcggtcgtt cctgaccggc
      481 cagaccggct cggcttgctc ggcttggcgg gcttgctcgg cttgccgccc ttggccggct
      541 tggatcgggc gcccggcttg ggagcagccg tcttcttctg cggccggcgc ttgccggcgg
      601 cctcggccgc ttccccgtcg gactgtttct ttgccatcgc ggcgtgtcta ccgtgcctcg
      661 cgaagctgcg tcaaggcaac cacacccgcg gccgacgtgg acgaaaaatt gcccccgatc
      721 gggctggccg tttagcgtcg gaatggcgac gggacggccg cgcgttctcg tcgtcaaccg
      781 ggccggctgc cgccggggcc gcggccacgg ccaaacgaag gagatgtcag atgcagtccc
      841 gcaagatcgg tgtgttgctc ggagggatgt ccagcgagcg cgaggtatcc ctgcgcacgg
      901 gagaggcagt gctcgcagcg ctgcgcgagc gcggccaccg ggcgatcccg atctatgtcg
      961 atcgcgacgt cgacgtggcg ctccgccagg agcagatcga ggtcgcgttc atcgcgctcc
     1021 acggccggtg gggggaggac ggctgcatcc agggcctgct ggagatgctc gggatcccct
     1081 acactggctc cgatgtcctc gcatcggcgc tggcgatgca caaggtcaag gccaaggagc
     1141 tgttccggct ccacaacctg ccgactcccg cgtactatac gctcaccggc gcccagctcg
     1201 ccgacggccc cgaagaaacc ccggccgacg cgctcgcggc gatccacggc gatttcgggt
     1261 tcccgtgcgt ggtcaagccg gtccgcgagg gcagctcggt cggcgtctcg atctgccgcg
     1321 acatggccga gctcggcccc gcggccctgc gcgcgctgcg gttcgacgac gagatcctcg
     1381 tcgagcggtt catcgccggc cgcgagatct cggtcgcggt gctcggcgac cgcgcgatcg
     1441 gggcggtcga gatcgcgccg cgtgacgggt tctacgacta cgccaacaag tacatccggg
     1501 gcgccaccga ctactacgtg ccgccccggg tgtctccgga gcgctaccgc ggcctgctga
     1561 cccaggcgct gcgcgcccac ctggcgctcg gctgcagcgg cgtgacccgc gtcgacctga
     1621 tgctcagcga ggcgggcaac gagttcattc tcgaggtcaa cacgctgccc gggctgaccc
     1681 cgaccagcct gctgcccaag atcgccgacg ctgcggggat ctcgttcggc gagctgtgcg
     1741 agatgatgct gtccggcgct agcctggcca cccggcgcgg tcgcggcgag cgccggatcg
     1801 cccagcgcac gttcgtcggc gatgatcgcc gccagggcga cgcggtggcc gagcgctacg
     1861 accaccatta gccgaggtgc c
//
LOCUS       DASVOR010001792         3627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_76929,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001792 DASVOR010000000
VERSION     DASVOR010001792.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76929"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            708..2222
                     /locus_tag="VF516_33350"
     CDS             708..2222
                     /locus_tag="VF516_33350"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8112681.1"
                     /translation="MKRAILFLVLAACGSPGAPPVAAPDTPTSRSNPRERLAACHIDG
                     VTGPVLCGSHAVWENRATRQGRKIELRVVVLPALGPDAAPDPVFEFAGGPGDAAGNAV
                     RGWAQDRETRAHRDIVLVDQRGTGGSHRLDCKIPRDPADVQAYFEPSLPPAEVHRCRG
                     ELEQVADLAQYTTSIAADDLDEIRGWLGYDKINLHGGSYGTRAAEVYLRRHGDHVRSA
                     FLIGVAAMDQYLPLFHARDGKHSLDLLFETCARTPACAAAFPDLPAEHERVLAALDRE
                     RGRATVDSPVDHHPVEATIPRDIFAEQIRFSLYSAGVASVTPYVIHRAAQGDFEPFAR
                     MAMSWEPSFRGILAYGMHLSVTCAEDVPFITPAMVESATAGTYLRGYRVLQQITACRD
                     WPRGEIPAGYHQPVRSDVPVLLVSGPYDPVTPPRFAAEVAKSLPHAVQLTVPDGHHGP
                     GGLAHPECFGGITARFFERGTADGLDTTCVAGMTRPPFVTDAAVFTALLQSAND"
     gene            2297..>3627
                     /locus_tag="VF516_33355"
     CDS             2297..>3627
                     /locus_tag="VF516_33355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8112682.1"
                     /translation="MLTARRLTVLAALSIPFLARAQPAGIDPGINVGAPPGDIDPTLA
                     KVLATTPAIDNHAHPVLPPPHLATDRGFDALPVDNMEPQTDPVAWRPDSPQLPDAWKA
                     LWHFTEAPPLSADALKRLNAARDKVRAQQGERYDLWVLDQARIGTMLANRVALGQGAQ
                     PPRFRWVPYIDALVFPLDNSGLAANSPDRALFFPLEDKLRAQYLQAVHLQAVPPTLDG
                     YLSQVVTPTLERQHGEGAVAEKFEIAYLRGFDFTDVPRDQAAKIYARLVGRPHPDLAD
                     YKLLQDFLFRYIVEECGRLHMAVHLHAMSGAGGYFSIAGVHPLLLEPLFNAPRLRKTS
                     FVMLHGGWPYIHEIGALLQKPNAYLDLSQQSLVIPPRTLAGWLREWLEIFPDKVLFAT
                     DGYPFTDSLGWEEASYLASRNIRQALGLALTGMLRDHEISREQAAALARGVL"
BASE COUNT          498 a         1386 c         1228 g          515 t
ORIGIN      
        1 cggcggtgcg ggcaccgggt gggcacatgc aaacatcacg gcgagcaggg cggcgagggg
       61 cgggcggtgc acgctgatgg tacgagcgag cccccgctcc ggatccgcag atccccggcg
      121 atcggcgcgt ctcgacgaat cccgcggggc aagaaccatg tgcgggtcat gggcgtcgca
      181 cgcgcgagac ggtcttgccg gctcgcgtga aacctcgacg agacactccg tcgacgatcc
      241 gctgtctcct cggcgagaca gcctccctgt gtgcgatgcg cggatcccga tcggcgcgtc
      301 gcaactaccc ggcgccgcgt cgccgacgct ggcgcgccgc ttgcgatggc ggtgctcggt
      361 cattgccacc aaggagttcc atgactgcca ttctacgaat gcgggcgatt gccccgctgc
      421 tctcgctcgc tgcgtgcctg ccgcacgacg acaccttctc cggccgtgga acaacgtgac
      481 gcgcggtcag gccgcgaaga tggtcgccgc gaacggcgtc gactgcagct ggtaacaacc
      541 ggaaggggtg gggcagtgcg gcctgcacgc tccggcgcac gcgcggtgca tgtgcgcatc
      601 tcgttgtcat ccgcagcggc cccccgggcg tcctctatcg agcgacaatg cacaccgcgc
      661 cgcccggcgc gatccacggc ggaggtccaa ccttgcggag gtccacgatg aagcgcgcga
      721 tcttgttcct cgtcctggcg gcctgcggct cccccggggc gccgccggtc gccgccccgg
      781 atacccccac ctcgcggtcg aacccgcgcg agcggctcgc cgcgtgccac atcgacgggg
      841 tcaccggacc ggtgctgtgc ggcagccatg cggtgtggga gaaccgagcg acccggcagg
      901 gccgcaagat cgagctccgc gtcgtggtgc tgcccgcgct cggcccggac gccgcgcccg
      961 atccggtgtt cgagttcgcc ggcgggcccg gcgacgccgc cggcaacgcc gtgcgcggct
     1021 gggcccagga ccgcgagacc cgcgcccacc gcgacatcgt gctggtcgac cagcgcggca
     1081 ccggcgggtc gcatcgcctc gactgcaaga tcccgcgcga tccggccgac gtgcaggcgt
     1141 acttcgagcc gtcgctgccg cccgccgagg tccaccgctg ccgcggcgag ctcgagcagg
     1201 tcgccgacct cgcgcagtac acgacgtcga tcgccgcgga tgatctcgac gagatccgcg
     1261 gctggctcgg ctacgacaag atcaacctgc acggcgggtc ctacggcacc cgggcggccg
     1321 aggtctacct gcggcgccac ggcgatcacg tgcgcagcgc cttcctgatc ggcgtcgccg
     1381 cgatggacca gtacctgccg ctgttccatg cccgggacgg caagcattcg ctcgacctct
     1441 tgttcgagac ctgcgcgcgc actccggcgt gcgccgcggc gttccccgac ctgcccgccg
     1501 agcacgagcg ggtgctcgcc gccctcgacc gcgagcgcgg ccgcgcgacc gtcgacagcc
     1561 cggtcgacca ccatccggtg gaggcgacga tcccgcgcga catcttcgcc gaacagatcc
     1621 ggttctcgct gtacagcgcc ggcgtcgcct cggtgacgcc gtacgtcatc caccgcgccg
     1681 cgcagggcga cttcgagccg ttcgcgcgca tggcgatgtc gtgggagccg agcttccgcg
     1741 gcatcctggc gtacggcatg cacctgtcgg tgacctgcgc cgaggacgtc ccgttcatca
     1801 cgcccgcgat ggtcgagtcc gcgaccgccg ggacctacct gcgcggctac cgcgtgctgc
     1861 agcagatcac ggcgtgccgg gactggccgc gcggcgagat ccccgccggg tatcaccagc
     1921 cggtgcgctc cgatgtcccg gtgctgctgg tgtccgggcc gtacgacccg gtgacgccgc
     1981 cgcggttcgc cgccgaggtc gccaagtcgc tgccccacgc cgtccagctg accgtccccg
     2041 acggccacca cggtccgggc ggcctggcgc accccgagtg cttcggcggc atcaccgcgc
     2101 ggttcttcga gcgcggcacc gccgacggcc tcgataccac gtgcgtcgcc ggcatgacgc
     2161 gcccgccgtt cgtcaccgac gccgcggtct tcaccgcgct gctgcagtcc gccaacgact
     2221 gaccgcggac ggcggcggcg cgccgcgatg ccggtccagc ggcgcactcc cgctcagatc
     2281 ggataccgtg cgccccatgc tcaccgcacg ccggctgacc gtcctcgccg ctctctcgat
     2341 tcccttcctc gcccgggccc agcccgcggg catcgatccg gggatcaacg tcggcgcacc
     2401 tccgggcgac atcgatccga cgctcgccaa ggtgctggcc acgacgcccg cgatcgacaa
     2461 ccacgcgcac cccgtgctgc cgccgcccca cctcgcgacc gatcgcgggt tcgacgcgct
     2521 cccggtcgac aacatggagc cgcagaccga tccggtggcg tggcggccgg acagcccgca
     2581 gctgcccgac gcgtggaagg cgctgtggca cttcaccgag gcgccgccgc tgtccgccga
     2641 cgcgctcaag cggctgaacg ccgcccgcga caaggtccgc gcgcagcagg gtgagcgcta
     2701 cgacctgtgg gtgctcgacc aggcgcggat cgggacgatg ctggccaacc gggtcgcgct
     2761 cgggcagggc gcgcagccgc cgcggttccg ctgggtgccg tacatcgacg cgctggtgtt
     2821 cccgctcgac aactccggcc tggccgccaa ctcgcccgat cgcgcgctgt tcttcccgct
     2881 cgaggacaag ctgcgcgcgc agtacctgca ggcggtccac ctgcaggccg tgccgccgac
     2941 gctcgacggc tacctgagcc aggtcgtcac gccgacgctg gagcgccagc acggcgaggg
     3001 cgcggtcgcc gagaagttcg agatcgccta cctgcgcggc ttcgacttca ccgacgtgcc
     3061 ccgcgatcag gccgccaaga tctacgcccg gctggtcggg cgcccgcacc ctgatctggc
     3121 cgactacaag ctcctgcagg acttcctgtt ccggtacatc gtcgaggagt gcggccggct
     3181 gcacatggcc gtccacctcc acgcgatgag cggcgccggc gggtacttca gcatcgccgg
     3241 ggtccatccg ctgctgctcg agccgctgtt caacgcgccc aggctgcgca agaccagctt
     3301 cgtgatgctg cacggcggct ggccgtacat ccacgagatc ggcgcgctgc tccagaaacc
     3361 gaatgcctat ctcgatctgt cgcagcagtc gctggtgatc ccgccgcgca cgctcgccgg
     3421 ctggctgcgc gagtggctcg agatctttcc ggacaaggtg ctgttcgcca ccgacggcta
     3481 cccgttcacc gactcgctgg gctgggagga ggcgagctac ctcgccagcc gcaacatccg
     3541 ccaggcgctc gggctggcgc tcaccggcat gctgcgcgat cacgagatca gccgcgagca
     3601 ggcggctgcg ctcgcgcgcg gcgtgct
//
LOCUS       DASVOR010001793         5095 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_339595,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001793 DASVOR010000000
VERSION     DASVOR010001793.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5095
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339595"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1664)
                     /locus_tag="VF516_33360"
     CDS             complement(<1..1664)
                     /locus_tag="VF516_33360"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112683.1"
                     /translation="MSDHCKGAKPELVKLKLRAQPEYWQSLEERDHGVRDRGEFPGGL
                     PAGKDDTTDHDGDHTRRDFLGLMGFGAAAATMAACRAPETKAVPIAVATDDMVPGVPN
                     FYATTCGGCASACSLLVKQRDGRPIKVEGNELSTLFGGATCATGQATVLSLYDQERLR
                     QPMWDGKPAEGHDADLQWRDVDDKIAAGLEEARAKQGKVVLLSTTITSPTLLAVIDRW
                     RKAFPGFRHVTYDPMSASALRAAAAVAFGRPLIPHYAFDRAKVIVGIDADFLGTWLSP
                     VEFARQYARGRAPEQPPGLHVQLEPGMSVTGSNADQRIAVAPSQLGAVAAALLARIAR
                     KAGEADVTAPADPVKPAILDQVADELWKHRGDSLVVAGANDTATQLVVLTINRLLGNV
                     GKTLDVDRPSLQRQGDDDAMAQLVADMEGGNVHTVILHGVNPVYDYVDGAKFAAALSK
                     VPLSISTADRMDETAAKAKAVCPDHHFLESWGDAEPVAGMLSLTQPLIAPLFDTRSVV
                     DSLLRWLGEPGTHYEVLRDYWRTQVLPRQGGVTGARDFDGFWDQTL"
     gene            complement(1681..2259)
                     /locus_tag="VF516_33365"
     CDS             complement(1681..2259)
                     /locus_tag="VF516_33365"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX8112684.1"
                     /translation="MRPLIILIGLVIAVIVVATGVRRLFRLGMQQGYAPAQPIAFSHK
                     LHAGDNQVPCLYCHYGARSSRHAGIPSSSVCMNCHGLLEKQTIEIERFKEAAQLDKPV
                     VAWTKVHNLPDFVYFNHSRHVLKGIDCENCHGDVKTMARVEQVKELTMGFCLTCHRQH
                     AGVETNTLARAAKRFTEAPKPATGLDCASCHY"
     gene            complement(2281..3912)
                     /locus_tag="VF516_33370"
     CDS             complement(2281..3912)
                     /locus_tag="VF516_33370"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HEX8112685.1"
                     /translation="MALAIAAHTLLFAGVASADTAEAKKVFTTRCMACHTFGKGVKVG
                     PDLKGVTQRRDMAWIVKMVRSSSTMVGSDPIAVQLFEQFKQQRMPDWTDLSEAQVKDI
                     MAWLAANGPDQQDPDARSAELATAAEIQTGRELFHGMRPLSLGGLACASCHTIRDEAG
                     SGGGVLASDLTSTYSQYQDIAMTMFLKRPCFLRFPESNGPTYLAPEESFALKAYLRSC
                     ALKNQPAESSPGKPAAQPPMVAKPVDTGAQGSGSNGSAAAAQPASAGQRVVWTPGVTG
                     APLGGHRSTAVPSELLFQVFPYVALAILILGLGIRHALARRRPEAIRPAAESAWQLFR
                     GSAAWRIGLAITAILHVVCLAVPGAIRTWNGTPLRLYLLEGTGFVFGVIALIGLIQLL
                     WRHVGRSIAANHHRLLEIGDYALLSVLCVATVSGLAMAVMYRWGSSWAVGTLTPYLWS
                     LGKGEPAIELVEQMPFLTRLHVFSWFAVLAVVPFTSAAMILVAAGDRVVLAAGRPITA
                     VAQTGRRALGKLSPARWLWPEEDLPGDGGDAHEPS"
     gene            complement(4599..>5095)
                     /locus_tag="VF516_33375"
     CDS             complement(4599..>5095)
                     /locus_tag="VF516_33375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112686.1"
                     /translation="RAAHADDAPPAAPAPSQHPGVGGHFGVAVPLVFIRSENPPGNQT
                     VGDQFTLAFPIGIGFSLTDKVALDLETIVGNPISPRGTTGVTIDPGIVYNAGPVVLGG
                     RLGWDIGAPTNFKLIPLIHRAVANVGAGANWFVEAAFPITFDFPPGKTTTELDIVFHT
                     GIGF"
BASE COUNT          745 a         1740 c         1817 g          793 t
ORIGIN      
        1 tccagggtct ggtcccagaa cccgtcgaag tcacgcgcgc cggtcacgcc gccctggcgc
       61 ggcaggacct gggtgcgcca gtagtcgcgc agcacctcgt agtgggtccc cggctcgccg
      121 agccagcgca gcaggctgtc gaccaccgag cgggtgtcga acagcggcgc gatcagcggc
      181 tgggtcaggc tgagcatccc ggcgaccggc tcggcgtcgc cccacgactc gaggaagtgg
      241 tgatcggggc acaccgcctt ggccttggcc gcggtctcgt ccatccggtc ggcggtcgag
      301 atcgacagcg ggaccttgga cagcgcggcc gcgaacttgg cgccgtcgac gtagtcgtac
      361 accgggttga cgccgtgcag gatcaccgtg tggacgttgc cgccctccat gtcggcgacc
      421 agctgggcca tcgcgtcgtc gtcgccctgg cgctgcagcg atgggcggtc gacgtcgagc
      481 gtcttgccga cgttgccgag caaccggttg atcgtgagca cgaccagctg ggtcgcggtg
      541 tcgttcgcgc cggccacgac cagcgagtcg ccgcggtgct tccacagctc gtcggcgacc
      601 tggtcgagga tcgcgggctt gaccgggtcg gcgggcgcgg tgacgtcggc ctcgccggcc
      661 ttgcgtgcga tccgggccag cagcgcggcg gcgaccgcgc cgagctggga cggcgcgacc
      721 gcgatccgct gatcggcgtt gctgccggtg accgacatcc cgggctcgag ctggacgtgg
      781 agcccgggcg gctgctccgg ggcccggccg cgcgcgtact ggcgggcgaa ctcgaccggc
      841 gacagccagg tgccgaggaa gtcggcgtcg atccccacga tcaccttcgc gcggtcgaac
      901 gcgtagtgcg ggatcagcgg gcggccgaac gcgaccgcgg cggcggcgcg cagcgcgctc
      961 gccgacatcg ggtcgtaggt cacgtggcgg aagccgggga acgccttgcg ccagcggtcg
     1021 atcaccgcga gcagggtcgg gctggtgatc gtggtcgaca gcagcacgac cttgccctgc
     1081 ttggcgcgcg cctcttcgag cccggccgcg atcttgtcat cgacgtcgcg ccactggagg
     1141 tcggcatcgt ggccctccgc cggcttgccg tcccacatcg gctggcgcag ccgctcctga
     1201 tcgtagagcg acagcaccgt ggcctggccg gtggcgcagg tcgcgccgcc gaacagcgtc
     1261 gacagctcgt tgccctcgac cttgatcggc cggccgtcgc gctgcttgac cagcaggctg
     1321 cacgccgacg cgcagccgcc gcaggtcgtc gcgtagaagt tcgggacgcc cggaaccatg
     1381 tcgtcggtcg ccaccgcgat cgggaccgcc ttggtctccg gcgcgcggca cgccgccatc
     1441 gtcgcggcgg cggcgccgaa ccccatcagg ccgaggaagt cgcggcgcgt gtggtccccg
     1501 tcgtggtcgg tcgtgtcgtc cttgccggcc ggcagcccgc cggggaactc gccgcggtcg
     1561 cgcaccccgt gatcgcgctc ctccaggctc tgccagtact cgggctgcgc ccggagcttc
     1621 agcttcacga gctcgggctt ggcgcctttg caatgatcgc tcatgggatc cttccggctc
     1681 ctagtagtgg cagctcgcgc agtccaggcc ggtcgccggc ttgggcgcct cggtgaaccg
     1741 cttcgccgcc cgcgccagcg tgttggtctc gacgccggcg tgctggcggt ggcaggtcag
     1801 gcagaagccc atcgtcagct ccttgacctg ctcgacgcgc gccatggtct tgacgtcgcc
     1861 gtggcagttc tcgcagtcga tgcccttcag cacgtggcgg ctgtggttga agtacacgaa
     1921 gtcgggcagg ttgtggacct tggtccacgc gacgaccggc ttgtcgagct gggcggcttc
     1981 cttgaaccgc tcgatctcga tcgtctgctt ctcgagcagg ccgtggcagt tcatgcacac
     2041 gctcgacgac gggatcccgg cgtggcggct cgaccgcgcg ccgtagtgac agtacaggca
     2101 gggcacctgg ttgtcgccgg cgtggagctt gtgcgagaac gcgatcggct gcgcgggcgc
     2161 atagccctgc tgcatgccca agcggaacag acgcctgacg ccggtcgcga cgacgatcac
     2221 cgcgatgacc aggccgatga ggatgatcaa tggtctcatg gcagattgtc tctggctgga
     2281 tcagctcggt tcgtgtgcgt cgccgccgtc gcccgggagg tcttcttccg gccacagcca
     2341 gcgcgccggg ctgagcttgc ccagcgcgcg ccgtccggtc tgcgccaccg ccgtgatcgg
     2401 ccgccccgcg gccagcacga cgcggtctcc cgccgccacc aggatcatcg cggcgctggt
     2461 gaacggcacc accgcgagca cggcgaacca cgagaacacg tggagccggg tcaggaatgg
     2521 catctgctcg accagctcga tcgcgggctc tcccttgccg agggaccaca ggtacggcgt
     2581 cagcgtgccg accgcccacg acgagcccca gcggtacatc acggccatcg cgagccccga
     2641 caccgtcgcc acgcacagca ccgagagcag cgcgtagtcg ccgatctcga gcagccggtg
     2701 gtggttcgcg gcgatgctgc ggccgacgtg ccgccacagc agctggatca ggccgatcag
     2761 cgcgatgacg ccgaacacga agccggttcc ctcgagcagg tacagccgca gcggcgtgcc
     2821 gttccaggtc cggatcgcgc cgggcacggc caggcacacc acgtgcaata tcgccgtgat
     2881 cgcgagcccg atccgccacg ccgcgctgcc gcggaacagc tgccaggccg actcggcggc
     2941 gggccggatc gcctcgggac ggcggcgcgc cagcgcgtgg cggatgccga ggccgaggat
     3001 caggatcgcg agcgcgacgt aggggaatac ctggaacagg agctcgctcg gcaccgcggt
     3061 gctgcggtga cccccgagcg gtgcgccggt cacgccgggg gtccacacca cgcgctgacc
     3121 cgcgctcgcc ggctgggcgg cggccgccga accgttcgag ccgctgccct gcgccccggt
     3181 gtcgaccggc ttggcgacca tcggcggctg ggcggcgggc ttgccgggcg acgactcggc
     3241 cggctggttc ttgagcgcgc agctccgcag gtaggccttg agcgcgaacg actcctcggg
     3301 cgcgaggtac gtcggcccgt tcgactcggg gaaccgcagg aagcagggcc gcttgaggaa
     3361 catcgtcatc gcgatgtcct ggtactgcga gtacgtgctg gtcaggtcgc tcgcgagcac
     3421 gccgccgccc gatccggctt catcgcggat cgtgtggcag ctcgcacagg cgaggccgcc
     3481 gagcgagagc gggcgcatgc cgtggaacag ctcgcggccg gtctggatct cggcagccgt
     3541 tgccagctcg gcggaccggg cgtccggatc ctgctggtcc gggccgttgg cggccagcca
     3601 ggccatgatg tccttgacct gggcctccga gaggtcagtc cagtccggca ttcgctgctg
     3661 cttgaactgc tcgaacagct gcaccgcgat cgggtccgac ccgaccatcg tgctcgacga
     3721 gcgcaccatc ttgacgatcc acgccatgtc ccgccgctgg gtcacgccct tgaggtcggg
     3781 accgaccttc acgcccttgc cgaacgtgtg gcaggccatg cagcgcgtgg tgaagacctt
     3841 cttggcctct gcggtgtcgg cgctcgcaac ccctgcgaac agcagtgtgt gggcagcgat
     3901 ggccagcgcc aagaaactga cacccacacg caatgtgcgg gtatagacca gtaaccgtcc
     3961 aaacatcacg ataggcccct ccgtcgagtg gatcgcggtc ttcgtgcctg tgctgcgtct
     4021 gtgtgtgatt cggtgtcgcg caatcgaaaa atgacccgag tgccgcggat taagtcagaa
     4081 cggttctaac cgtgcaagcc cagaatttag actctctgtc tcggatcagg tcgacgatga
     4141 cccgctcgta tcaggaattt gagagtcgcg agccgatcga gcgcgggtcg ggacgtgcag
     4201 caatgcgcgt ccgtccggtc gcgcggagcg agctaacgcc ggtcaatcgc tctcgatcga
     4261 gagcgtgcgc cgctcgcgtc tccgcgcgcg agcgcgtggg cttcgggcac acaggcggcg
     4321 cgtcccgcgc accgagccgg cgccttaatg ttccttcatg cagggggaac cgcgacagaa
     4381 aaccgcagct cggggcgggt gccgtggccg tgatccgccc ggccccgact cggcgaatct
     4441 ctcagccaga aatgcgagta ttttcgagcg acaacatcgg gatcaggccc gatgcgacaa
     4501 attgtccgta acgattcgtg gaccctgtcg cacggaagtc gcagacgctc cgatcccgcc
     4561 gagcgtgcgc ctacgcgcag cggttgcgcg aaacgcgttc agaacccgat tccggtgtgg
     4621 aacacgatgt cgagctcggt ggtggtcttg cccggcggga agtcgaacgt gatcgggaac
     4681 gcggcctcga cgaaccagtt cgcgccggcg ccgacgttcg cgacggccct gtggatcagc
     4741 ggaatcagct tgaagttggt cggtgcgccg atgtcccagc cgagccgccc gccgagcacg
     4801 accgggccgg cgttgtagac gatgccgggg tcgatggtga cgccggtggt gccgcgtggg
     4861 ctgatcggat tgccgacaat ggtctcgagg tcgagcgcga ccttgtcggt caggctgaag
     4921 ccgatcccga tcgggaatgc gagcgtgaac tggtcaccga ccgtctggtt gccgggcggg
     4981 ttctcgctgc ggatgaacac cagcgggacg gcgacgccga agtggccgcc gacgccgggg
     5041 tgctggctcg gggcgggggc ggccgggggc gcgtcgtcgg cgtgcgccgc gcgcg
//
LOCUS       DASVOR010001794         1642 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_164465,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001794 DASVOR010000000
VERSION     DASVOR010001794.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164465"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..>1642)
                     /locus_tag="VF516_33380"
     CDS             complement(127..>1642)
                     /locus_tag="VF516_33380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112687.1"
                     /translation="GPLGAGNWLFDRLAIPGIADMLAINPGVDGVGADLGGRSVLLLP
                     HELVGSNLLAAKRPSELMPDFAMGVDLNGISRLLASRTGAPGAAALPPGALYRFRTDT
                     FVEPPDHAGPPLPLYRGLPPAPEVSARTLREAALRGGRYLVAHLAPSGRYVYQHDLWT
                     GAQTDATRPGPYSMPRHAGTTYFLAELYRITRESWLREPVERAFAHLADLLGHGGCAT
                     ALPDGTAVDCVVDRGETVAHLGSTALAVVALVEYQRATGDARYLPLATRLAAWLLFMQ
                     RPDGSFRHVYDVATRTPDEAAQLLYYSGEAALALARMHAITGEARYAAAAERALDWLV
                     DWYDFFMGGFFYGEEHWTCIAAEAIWLAVRKDRYAAFCDGYGRFVRAQQFEPGEHPDE
                     DDFTGAYGLTPFIAPVNTPTGSHTEALISTYLLGRHRGAPDPRLAAQIRAALQYLLGQ
                     QIRPDSAFDVVGPGEGAVPETVIARNIRIDYVQHVCSAMIRASEWIDGPDPAAR"
BASE COUNT          221 a          551 c          656 g          214 t
ORIGIN      
        1 ccgccgctgc caccgccctg ctcggcccgg tcaccggacg gacgggaccg gtgggaccta
       61 tctgggtccg cttgtcttct ggcaccgctc gcatcatagc agggtggcct tgtgatctgg
      121 gatgcgttat cgcgcggccg gatcagggcc gtcgatccac tccgaggcgc ggatcatcgc
      181 cgagcacacg tgctgaacgt agtcgatgcg gatatttcgg gcgatcaccg tctcggggac
      241 cgcgccctcg cccggcccca ccacgtcgaa cgcgctgtcc ggccggatct gctggccgag
      301 caggtactgc agcgccgcgc ggatctgggc ggcgagccgg ggatccgggg cgccgcggtg
      361 ccggccgagc aggtacgtcg agatcagcgc ctcggtgtgc gatccagtgg gggtgttgac
      421 cggcgcgatg aagggggtga ggccgtaggc gccggtgaag tcgtcctcgt cggggtgctc
      481 gccgggctcg aactgctggg cgcggacgaa ccggccgtag ccgtcgcaga acgcggcgta
      541 gcggtccttg cgcaccgcga gccagatcgc ctcggcggcg atgcatgtcc agtgctcctc
      601 gccgtagaag aacccgccca tgaagaagtc gtaccagtcg accagccagt cgagcgcgcg
      661 ctcggcggcg gcggcgtagc gcgcctcgcc ggtgatcgcg tgcatccgtg cgagcgcgag
      721 cgcggcctcg ccggagtagt acagcagctg ggcggcctca tcgggggtgc gggtcgcgac
      781 gtcatagacg tggcggaacg agccgtcggg gcgctgcatg aacagcagcc acgcggcgag
      841 ccgggtcgcg agcggcaggt agcgggcgtc gccggtggcg cgctggtact cgacgagcgc
      901 gaccacggcg agcgcggtcg agccgaggtg ggcgacggtc tcgccgcgat cgacgacgca
      961 atccaccgcg gtgccgtcgg gcagcgcggt ggcgcagccg ccgtgcccga gcaggtccgc
     1021 caggtgggcg aacgcgcgct cgaccggctc gcgcagccag ctctcgcggg tgatgcggta
     1081 cagctcggcg aggaagtagg tcgtgccggc gtggcgcggc atgctgtacg ggccgggccg
     1141 ggtcgcgtcg gtctgcgcgc cggtccacag gtcgtgctgg tagacgtagc ggccgctcgg
     1201 ggcgaggtgg gcgaccaggt agcgcccgcc gcgcagcgcc gcctcgcgca gcgtccgggc
     1261 ggagacctcg ggggccggcg gtagcccgcg gtacagcggc agcggcgggc cggcgtggtc
     1321 cgggggctcg acgaacgtat cggtgcggaa ccggtacagc gcgccgggcg ggagcgccgc
     1381 ggcgccgggc gccccggtcc ggctggcgag caggcggctg attccgttca ggtccacgcc
     1441 catcgcgaag tcgggcatca gctcggacgg gcgcttggcg gcgagcaggt tcgacccgac
     1501 cagctcgtgc ggcaggagca gcaccgaccg gccaccgagg tcggccccga cgccatcgac
     1561 cccggggttg atcgcgagca tgtcggcgat gccggggatc gcgagccgat cgaacagcca
     1621 gttgccggcg ccgagcgggc cg
//
LOCUS       DASVOR010001795         2804 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_311181,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001795 DASVOR010000000
VERSION     DASVOR010001795.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311181"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VF516_33385"
     CDS             <1..238
                     /locus_tag="VF516_33385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEX8112688.1"
                     /translation="LVRCVARTGRMHQVRAHLAHVGAPLTGDALYGGAPLPGHDGFFL
                     HAERVALPLGGEQLVVTAPIPERFARALAACGAT"
     gene            285..491
                     /locus_tag="VF516_33390"
     CDS             285..491
                     /locus_tag="VF516_33390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112689.1"
                     /translation="MKRSLIATLFLITMIAVASGSRAAPSAAPEAPQIGSCRWFCTTT
                     GKAFSTASACATACGGPAACDEVC"
     gene            complement(502..1266)
                     /locus_tag="VF516_33395"
     CDS             complement(502..1266)
                     /locus_tag="VF516_33395"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HEX8112690.1"
                     /translation="MADLARLQQQFYDRVVGRAPGDGDDDPGDAGLIGSGDIAIYARM
                     YASRLHDTLADDYPKLRTALGDDRFGELVAHYLRAHPPSSFTLRDAGLALERFLRGSD
                     LAPPWAADLVALERARVEVFDGPDAEPLTQDAVAALGDALPGLVLAWIPSSVVVPLVW
                     TVDDLWSAIEDGAPTAPPAAEPRVVLVWRRDVAVLHRTLDADEARLAPRIAQGVSFAD
                     ACELLGELHGDAASARAVELLLRWLGAAVLAPPAAE"
     gene            complement(1286..1579)
                     /locus_tag="VF516_33400"
     CDS             complement(1286..1579)
                     /locus_tag="VF516_33400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112691.1"
                     /translation="MKNPIRTGNMIAFAAASLFAAACGSKAPDQSTTPAASPEKTAAA
                     VHCMGINSCKGMGSCKSEKNSCKGQNGCKGQGRMETATAEECTSKGGTVAAGN"
     gene            complement(1790..>2804)
                     /locus_tag="VF516_33405"
     CDS             complement(1790..>2804)
                     /locus_tag="VF516_33405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112692.1"
                     /translation="RGCVRRDSALQLSMRRSPLALALLLGITTCGGTSSIAAGAPRTA
                     PAAPAATAAPVTLAIGGGAIDIAIEDGPLDRSRAELLDWISSAARAVTAYFGRFPVAH
                     YRIVIEPVAGRSGVLSGTTWAYGGAHSRIHIGEHTSVAQLERDWIMTHEMVHTAFPQQ
                     ERSHHWIEEGMATYVEPLARSWIGHYPVARVWTDLVDGLPNGLPRRGDRGLDHTQTWG
                     RTYWGGALFCLLADLEIRDRTGGRRGLVDALRGILAAGGSDQVDWPIERAFREGDKAV
                     GVPVLEELYRRMKDAPIAPDLDALWRGLGIEVRQGTLQLDDQAPKAAMRRAISAPPVP
                     ATP"
BASE COUNT          378 a         1036 c          986 g          404 t
ORIGIN      
        1 gctggtccgc tgcgtcgccc gcaccggccg gatgcaccag gtccgcgccc acctcgcgca
       61 cgtcggcgcg ccgctcaccg gcgacgcgct gtacggcggt gcgccgctgc ccggccacga
      121 cggcttcttc ctccacgccg agcgcgtcgc gctgccgctc ggcggcgagc agctggtcgt
      181 cacggccccg atccccgagc ggttcgcgcg tgcactcgcc gcgtgcggcg caacctgaga
      241 tcgattatcg tcgatcctgg aggcattcct ccggaggtag taacatgaag agatcattga
      301 tcgccacgct gttcctgatc accatgatcg cggtcgcgag cgggtcccgc gctgcgccgt
      361 ccgccgcgcc cgaggctccg cagatcggaa gctgccgctg gttctgcacc accaccggca
      421 aggcgttctc gacggcgagc gcctgcgcca ccgcgtgtgg gggaccggcg gcctgcgacg
      481 aggtctgctg agcccgaccg gtcactcggc cgccgggggt gccagcaccg cggccccgag
      541 ccagcgcagc aagagctcga ccgcgcgggc gctcgccgcg tcgccgtgga gctcgccgag
      601 cagctcgcat gcatcggcga acgacacgcc ctgcgcgatc cgcggggcga gccgggcctc
      661 gtcggcgtcg agcgtgcggt gcagcaccgc gacatcgcgg cgccacacca gcacgacgcg
      721 cggctcggcg gcgggcgggg cggtgggcgc gccgtcctcg atcgcgctcc acaggtcgtc
      781 gaccgtccac accagcggga cgaccacgct cgacgggatc cacgccagca ccaggccggg
      841 cagcgcatcg cccagcgcgg cgaccgcgtc ctgggtcagc ggctccgcgt cgggcccgtc
      901 gaacacctcg acccgggcgc gctccagcgc gaccagatcg gcggcccacg gcggggcgag
      961 gtcgctgccg cgcaggaatc gctcgagcgc gaggcccgcg tcgcgcagcg tgaacgagct
     1021 cggcggatgc gcgcgcaggt agtgcgcgac cagctcgccg aaccgatcgt cgccgagcgc
     1081 ggtgcgcagc ttgggatagt cgtcggcgag cgtgtcgtgc aaccggctcg cgtacatccg
     1141 cgcgtagatc gcgatgtcgc ccgagccgat cagcccggca tcgccggggt cgtcatcgcc
     1201 gtctccgggt gcacggccga cgacgcggtc gtagaactgc tgctggagcc gcgccagatc
     1261 ggccatcgtc gagatcgcct cgcgatcagt tgccggccgc gacggtgccg cccttggagg
     1321 tgcactcctc ggcggtcgcg gtctccatcc tgccctggcc cttgcagccg ttctggccct
     1381 tgcaggagtt cttctcgctc ttgcagctgc ccatgccctt gcaggagttg atgcccatgc
     1441 agtgaaccgc cgcggcggtc ttctcgggcg aggcggcggg cgtcgtcgac tggtcgggag
     1501 ccttgctgcc acaggcggcg gcgaacaggc tggccgcggc gaacgcgatc atgtttccgg
     1561 tacggatggg gttcttcatt gtctggtctc cttgaagtga acgagcagtg ctacgccgcg
     1621 cacagaccca ggtcttggcc cggcatattc cctacgcttg gtcgggacgg aggctacacc
     1681 tgccgtgcaa ccggggtgca acggacgccg gtcattcctg atgacttcac tccggatgat
     1741 ctcctgacga gcctgatcac gtgttcagca ggcgatggcg agtccgctat cacggagtcg
     1801 ccggtaccgg cggcgcgctg atcgcgcggc gcatcgccgc cttcggcgcc tgatcgtcga
     1861 gctgcagcgt accctggcgc acctcgatgc cgaggccccg ccacagcgcg tccagatccg
     1921 gcgcgatcgg cgcatccttc atccgccggt agagctcctc gagcaccggc acgccgaccg
     1981 ccttgtcgcc ctcgcggaag gcgcgctcga tcggccagtc gacctgatcg ctgccgccgg
     2041 cggcgaggat gccgcgcagc gcgtcgacca ggccgcggcg gcctcccgtg cggtcccgga
     2101 tctccaggtc ggcgagcagg cagaacagcg caccgcccca gtaggtccgg ccccaggtct
     2161 gggtgtgatc gagtccacga tcaccccggc gcggcaggcc gttgggcagt ccatcgacca
     2221 gatcggtcca cacccgcgcg accgggtagt gaccgatcca cgatcgcgcc agcggctcga
     2281 cgtaggtggc catcccctcc tcgatccagt ggtgcgatcg ctcctgctgg ggaaacgcgg
     2341 tgtgcaccat ctcgtgcgtc atgatccagt cgcgctcgag ctgcgcgacg ctggtgtgct
     2401 cgccgatgtg gatccgcgag tgcgcgccgc cgtacgccca ggtcgtcccg ctgagcacgc
     2461 ccgaccggcc ggccaccggc tcgatcacga tccggtagtg cgcgaccggg aaccgcccga
     2521 agtacgcggt caccgcgcgg gcggcgctcg agatccagtc gagcagctcg gcgcgcgacc
     2581 ggtcgagcgg accgtcctcg atcgcgatgt cgatcgcgcc gccgccgatc gccagcgtga
     2641 cgggcgcggc cgtcgcggcc ggcgcggccg gcgcggttct tggcgccccg gccgcgatcg
     2701 acgaggtccc gccgcaggtc gtgatgccga gcagcagcgc gagggcgagc ggcgagcggc
     2761 gcatgctcag ctgtagcgcg gaatcgcggc gcacgcagcc ccgc
//
LOCUS       DASVOR010001796         1817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_252789,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001796 DASVOR010000000
VERSION     DASVOR010001796.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1817
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_252789"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..917)
                     /locus_tag="VF516_33410"
     CDS             complement(<1..917)
                     /locus_tag="VF516_33410"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8112693.1"
                     /translation="MLAELEPLAMSRTAYGVVIALGLLWGSFANVCIYRWPAGRSVVT
                     PGSHCMACQAPIRWYDNVPVLAWLWLRGRCRACKAAFSPRYLVIEAVTGALFGFAWWA
                     TVIAPALFEPFGARLVHFSIDAAFCFAMVVIAFIDIDHKLILNKVTIPSIALAYLASF
                     AWPGRRWYDGLIGAVVGYGVPWLIGALYYLIRRREAMGLGDSMLLAVVGALLGWRGVV
                     VALCVGSMIGTVVVLTALLARRVGGEPPPTDAPAAADSDRDPGAAEPAAGEPEKPSFL
                     FTEVPFGAFLAMAAVLYLFADPWVDIHFHL"
     gene            1004..>1817
                     /locus_tag="VF516_33415"
     CDS             1004..>1817
                     /locus_tag="VF516_33415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112694.1"
                     /translation="MIRFTVLVLCAAACGRSRGVQDQDLPGLVVEARKPEAPIDVARA
                     ARDPVELGRALARPYRVVLAALGPHTAGVNSSITVTEGDKPVSELSDHAQIDNGTGDA
                     YHAIYTNSADYGRETTFVDGTLYLRPRYQRWHARDPETPEEPAELRDRYFEAIAATWD
                     LLAPGAELADRGALEVAGRAGRKIEVAHTADPRDPAAEPLAQRKWREARSVDEVTGEI
                     VLDAERGVPLAVKLTGTVGFTRDGRRFAMQVSVDGAISGIGTAAMIQPPPQGE"
BASE COUNT          303 a          680 c          635 g          199 t
ORIGIN      
        1 aggtggaagt ggatgtcgac ccacggatcg gcgaacaggt acagcaccgc cgccatcgcg
       61 aggaacgcgc cgaacggcac ctcggtgaac aggaatgagg gtttctcggg ttcacccgcg
      121 gcgggctccg cggccccggg atcgcgatcg ctgtccgctg cggccggcgc gtccgtgggg
      181 ggcggctcac cgcccacccg gcgggccagc agcgcggtga ggacgacgac cgtgccgatc
      241 atcgagccga cgcacagcgc gaccacgacg ccgcgccagc cgagcagcgc gccgaccacc
      301 gccagcagca tgctgtcgcc gagccccatg gcctcgcgcc gccggatcag gtagtagagc
      361 gcaccgatca gccacggcac gccatagccg accaccgcgc cgatcaggcc gtcgtaccac
      421 cgccggcccg gccacgcgaa ggacgccagg taggccagcg cgatcgacgg gatcgtgacc
      481 ttgttcagga tcagcttgtg gtcgatgtcg atgaacgcga tcaccaccat cgcgaagcag
      541 aacgcggcat cgatcgagaa gtgcacgagc cgcgcgccga acggctcgaa cagcgcgggg
      601 gcgatcaccg tcgcccacca cgcgaagccg aacagcgcgc cggtcaccgc ctcgatgacc
      661 aggtagcgcg gcgagaacgc cgccttgcac gcccggcacc ggccgcgcaa ccagagccac
      721 gccagcaccg gcacgttgtc gtaccagcgg atcggcgcct ggcaggccat gcagtgcgag
      781 cccggcgtca ccaccgagcg ccccgccggc cagcgataga tgcagacgtt ggcgaagctg
      841 ccccacagca ggccgagcgc gatgacgacc ccgtaggcgg tcctcgacat cgccaggggt
      901 tcgagctcgg ccagcaccgc ggggtaacag tagccggtcc gccgcccgcg ggcgagctgc
      961 ccgccagttg accaggctcg tcggcaaagc taagaaacca gcgatgatcc ggttcaccgt
     1021 cctggtgctg tgcgctgccg cctgcggtcg gtcgcgtggg gtgcaggatc aggacctccc
     1081 cggcctggtg gtggaggcgc gcaagcccga ggcgccgatc gacgtggcgc gcgccgcccg
     1141 cgacccggtg gagctcggcc gggcgctggc gcggccgtac cgggtggtcc tcgcagcgct
     1201 ggggccgcat accgccgggg tcaacagctc gatcaccgtg accgagggcg acaagccggt
     1261 cagcgagctg tccgaccacg cccagatcga caacggcacc ggcgacgcct accacgcgat
     1321 ctacaccaac tccgccgact acggccgcga gaccacgttc gtcgacggca cgctgtacct
     1381 gcgaccgcgc taccagcgct ggcatgcgcg cgatccggag acccccgaag agccggccga
     1441 gctgcgcgat cgctacttcg aggcgatcgc cgcgacctgg gacctgctcg ccccgggcgc
     1501 cgagctcgcc gaccgcggcg cgctcgaggt cgccggacgc gccggccgca agatcgaggt
     1561 cgcccacacc gccgatccgc gcgatccggc cgccgagccg ctcgcgcagc gcaagtggcg
     1621 cgaggcgcgc agcgtcgacg aggtgaccgg cgagatcgtg ctcgacgccg agcgcggcgt
     1681 gccgctcgcg gtcaagctga ccggcaccgt cgggttcacc cgcgatggcc ggcggttcgc
     1741 catgcaggtc tcggtcgacg gcgcgatctc gggcatcggc accgccgcga tgatccagcc
     1801 cccgccccag ggcgagg
//
LOCUS       DASVOR010001797         6766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_280677,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001797 DASVOR010000000
VERSION     DASVOR010001797.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6766
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280677"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VF516_33420"
     CDS             <1..861
                     /locus_tag="VF516_33420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8112695.1"
                     /translation="DELDRGVHDGVPVDPLRLGAPLPRAYEWVDGSAFLNHVILVRRA
                     RGAQPPATLETDPLIYQGGSGDMLGPRDPIELCDPAWGLDYESEVCVVLDDTPLGTTA
                     ADAARYVRLVMLANDCTLRNLVPDELAKGFGFFQSKPATAFSPFAVTPDELEVTGSWR
                     GGRLYGRVRSTYNEAVIGDCETGPEMHFSFFDLIAHIARTRRFTAGTILGSGTVSNAD
                     PARGVSCLAEARMIEIIQSGKPATPFMAVGDRIRIEADVAGQSPFGAIDQRVVAAGAS
                     STQPPQGASP"
     gene            858..1505
                     /gene="maiA"
                     /locus_tag="VF516_33425"
     CDS             858..1505
                     /gene="maiA"
                     /locus_tag="VF516_33425"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEX8112696.1"
                     /translation="MKLVLHSYWRSSASHRVRIGLGLKQLPYDYVAVNIVHGEQLADA
                     YRAKNPMAQVPTLEVTEDDGTRFALTQSLAILEYLDERWPDPPLLPRDPLLRARARAL
                     AEIINAGIQPLQNLGTLRKIKSLGGDEAAWIRPVIADGLAAFARIAAEVSGTFSVGNA
                     PTIADCCLVPQLAGARRFQVDLEPFPRLLAIEAHCLPLPAFADAMPHHQPDAVKS"
     gene            1502..2689
                     /locus_tag="VF516_33430"
     CDS             1502..2689
                     /locus_tag="VF516_33430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8112697.1"
                     /translation="MTKPAPLGIKRLEGIHYYVRDLERSRRFYTEKLDFAETWRSSPE
                     LEARSGQRSVCFSAGAIDVVCSAPLGPVSDGRCRAARFLAKHPDGVGTLVFEVEDAAR
                     AFALLEQRGGTPIDDIQTFRDDGGTLSQFSITTPFGDCTFRFRERAGYRALYPGAVPH
                     RGGGANRFGFQHIDHVTSNFQTMKPMLLWLEHVLGFEQMWQVAFHTSDVDTVRASGSG
                     LKSIVMWDPVSQVKFANNEPARPYFKSSQINIFNEELRGDGVQHVALTVADIITAVRG
                     LRSAGVTFMPTPGSYYDLLPGRIAGSGIGAIDEDLATLRELQILVDGDHHHAYMLQIF
                     LKESAGLYADPSAGPFFYEIIQRKGDRGFGAGNFRALFESIEREQHEATGASAAAGAE
                     DGA"
     gene            2689..3846
                     /locus_tag="VF516_33435"
     CDS             2689..3846
                     /locus_tag="VF516_33435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homogentisate 1,2-dioxygenase"
                     /protein_id="HEX8112698.1"
                     /translation="MLDRMQLGDVPRKHHIQLRGPEGELRYEECFTRDGFDGPYTILY
                     HLRRPHTHRVTAPAHGWPAPAAAADRPLAKRHYRTGELARAAGAGPQIDVRVPLLFND
                     DLIAGVAFPSADDPVYVADGDADQLIYVHQGGGVLRSLLGDVRFAQGDYVFVPRGLLH
                     RFLPSGPQHWFWFSLTGGLHIPPQWRNPIGQLRMDAPYSHRDFKRPQLQPPLDEGIRD
                     LLVRRGGAWHGFTLDHPPLDVIGWDGTVYPWAFPILAFQPRVSSVHLPPTWHGTFAAR
                     GALICSFVPRPVDFHPEAIPCPYPHSSTHCDEIIFYCDGNFTSRRGVGPGSVSHHPMG
                     VPHGPHPGSYEKSIGATATSELAVMADSFAPLRATSAALTVEDAGYQDSFL"
     gene            3995..4249
                     /locus_tag="VF516_33440"
     CDS             3995..4249
                     /locus_tag="VF516_33440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112699.1"
                     /translation="MNDERTAVQRWLAGQRASARMQRELQAAEGPRPARAVAQSLAAL
                     AALETMGRWPGPRDAVSERAIDEVRRRWARIQRRARAARS"
     gene            4317..4952
                     /locus_tag="VF516_33445"
     CDS             4317..4952
                     /locus_tag="VF516_33445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112700.1"
                     /translation="MVIGGIAVIAHGVQRMTTDIDAVIQGDAVSVHTLIDVLQRHHIV
                     PRIADAEAFAATNLVLLTRHQPTEVDLDLSFGWTVFEHEALAARSRMRYGEVAAPMAG
                     VEELLVFKAMAGRPRDIEDAVTLLTLYPRIDLRSVRTRVATLAELAEAPELMLGFERI
                     VRAAAMPTKHTSPAKPPARRVRPGVVERTTPRPARPRAASGLGKKRRRSPR"
     gene            5122..6156
                     /locus_tag="VF516_33450"
     CDS             5122..6156
                     /locus_tag="VF516_33450"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEX8112701.1"
                     /translation="MRTTRAIQIVSCHAEGEVGDVIVGGVPPPPGDTLWEQSRFIATD
                     QTLRNFVLNEPRGGVFRHVNLLVPPRDPRAQMGFIIMEPEDTPPMSGSNSICVATVLL
                     DCGILAMTEPETRLVLEAPGGLVEVVAHCRSGKAERITVRNVASFADRLDATIEVAGL
                     GTLTADTAYGGDSFVIVDAHRLGFRLAPDEARDLAETGVRITRAANEQLGFVHPTNPG
                     WNHISFCQLAAPVAALAGVRTAANAVVVQPGKIDRSPTGTGASARMAVMRARGEMQVG
                     DRYLARSIIGSEFLGRIEADAEVGGRPAIIPSISGRAWITGTRQDMLDPDDPWPAGYR
                     LSDTWPRITR"
     gene            6216..>6766
                     /locus_tag="VF516_33455"
     CDS             6216..>6766
                     /locus_tag="VF516_33455"
                     /inference="COORDINATES: protein motif:HMM:NF028194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8112702.1"
                     /translation="MARKNHRFTLAARPTGLPRPTDFAFEEEAVRELGDRELLVKVQY
                     ISLDPAMRGWMNEGKSYIRPVAIGEVMRAGAIGSVIESKHPGFAAGTQVIGTFGLQEY
                     AISNGEGVTPIDPRLSPGLVPLPVMLSTLGMPGLTAYFGLLDIGKPVAGNTVVVSGAA
                     GAVGTVVGQIAKIHGCRAVGIAGG"
BASE COUNT          959 a         2545 c         2292 g          970 t
ORIGIN      
        1 gacgagctcg accgcggcgt gcacgacggc gtgccggtcg acccgctgcg gctgggcgcg
       61 ccgctgccgc gcgcctacga gtgggtcgac ggctcggcgt tcctcaacca cgtgatcctg
      121 gtccgccggg cgcgcggcgc ccagccgccg gccacgctgg agaccgatcc gctgatctac
      181 cagggcggct cgggcgacat gctcgggccg cgcgacccga tcgagctgtg cgatccggcg
      241 tggggcctcg actacgagtc cgaggtctgc gtggtgctcg acgacacgcc gctcggcacc
      301 accgcggcgg acgcggcgcg ctacgtccgg ctggtcatgc tcgccaacga ctgcacgctg
      361 cgcaacctcg tccccgacga gctcgccaag ggcttcgggt tcttccagag caagccggcg
      421 accgcgttct cgccgttcgc ggtcaccccg gacgagctcg aggtcaccgg cagctggcgc
      481 ggcggccggc tgtacggccg ggtccgctcg acctacaacg aggccgtgat cggcgactgc
      541 gagaccggac ccgagatgca cttctcgttc ttcgatctga tcgcgcacat cgcccggacc
      601 cggcggttca ccgcggggac catcctcggc agcggcacgg tgtcgaacgc cgacccggcg
      661 cgcggcgtgt cgtgcctggc ggaagcccgg atgatcgaga tcatccagtc gggcaagccg
      721 gcgacgccgt tcatggcggt cggcgaccgg atccggatcg aggccgacgt cgccggccag
      781 agcccgttcg gcgcgatcga ccagcgcgtg gtcgcggcgg gagcatcctc cacccagcca
      841 ccgcagggag cgtccccatg aagctcgtcc ttcacagcta ctggcgcagc tcggcgagcc
      901 accgcgtgcg gatcggcctc ggcctcaagc agctgccgta cgactacgtg gcggtcaaca
      961 tcgtgcacgg cgagcagctc gccgacgcct accgggccaa gaacccgatg gcgcaggtcc
     1021 cgaccctcga ggtcaccgag gacgacggca cccgcttcgc gctgacccag tcgctcgcga
     1081 tcctcgagta cctcgacgag cgctggcccg atccgccgct cctgccccgc gatccgctgc
     1141 tccgcgcccg ggcgcgcgcg ctcgccgaga tcatcaacgc cgggatccag ccgctgcaga
     1201 acctgggcac gctgcgcaag atcaagtcgc tgggcggcga cgaggcggcg tggatccggc
     1261 cggtgatcgc cgacggcctg gccgcgttcg cccggatcgc cgccgaggtc tccggcacgt
     1321 tctcggtcgg caacgccccg acgatcgccg actgctgcct cgtgccgcag ctcgccggcg
     1381 cgcgccggtt ccaggtcgac ctcgagccgt tccccaggct gctcgcgatc gaggcccact
     1441 gcctcccgct gccggcgttc gccgacgcga tgccacacca ccaacccgac gccgtgaagt
     1501 catgaccaag ccggccccgc tgggcatcaa gcgcctcgaa ggcatccatt actacgtgcg
     1561 cgacctcgag cgctcgcgcc ggttctacac cgagaagctc gacttcgcgg agacctggcg
     1621 gtcgtcgccc gagctcgagg cgcgcagcgg ccagcgctcg gtgtgcttct cggccggcgc
     1681 gatcgacgtc gtgtgcagcg cgccgctcgg cccggtcagc gacggccgct gccgcgccgc
     1741 ccggttcctc gccaagcacc ccgacggcgt cggcaccctg gtgttcgagg tcgaggacgc
     1801 cgcgcgcgcg ttcgcgttgc tcgagcagcg cggcgggacg ccgatcgacg acatccagac
     1861 gttccgcgac gacggcggca cgctgagcca gttctcgatc accaccccgt tcggcgactg
     1921 cacgttccgg ttccgcgagc gcgccggcta ccgcgcgctg tacccgggcg ccgtgccgca
     1981 ccggggcggc ggcgccaacc ggttcggctt ccagcacatc gaccacgtga cctcgaactt
     2041 ccagaccatg aagccgatgc tgctgtggct ggagcacgtc ctgggcttcg agcagatgtg
     2101 gcaggtcgcg ttccacacct ccgatgtcga cacggtacgc gcgtccggct cggggctcaa
     2161 gtcgatcgtg atgtgggacc cggtgtcgca ggtcaagttc gccaacaacg agccggcgcg
     2221 gccgtacttc aagagctcgc agatcaacat cttcaacgag gagctgcgcg gcgacggcgt
     2281 gcagcacgtc gcgctcaccg tcgccgacat catcaccgcg gtgcgcggcc tgcgcagcgc
     2341 cggcgtcacc ttcatgccca cgcccggcag ctactacgac ctgctgccgg gccggatcgc
     2401 cggaagcggc atcggcgcga tcgacgagga tctcgccacc ctgcgcgagc tccagatcct
     2461 ggtcgacggc gatcaccatc acgcctacat gctgcagatc ttcctcaagg agtcggccgg
     2521 cctgtacgcc gatccgagcg ccggcccgtt cttctacgag atcatccagc gcaagggcga
     2581 ccgcgggttc ggcgccggca acttccgggc gctgttcgag agcatcgagc gcgagcagca
     2641 cgaggccacc ggcgcctccg cggcggccgg cgccgaggac ggggcctgat gctcgaccgc
     2701 atgcagctcg gcgacgtccc gcgcaagcac cacatccagc tgcgcggccc cgagggcgag
     2761 ctccgctacg aggagtgctt cacccgcgac ggcttcgacg gcccgtacac catcctctat
     2821 cacctgcgcc ggccgcacac ccaccgggtc accgcgcccg cgcacggctg gcccgccccg
     2881 gccgccgccg ccgaccgccc gctcgccaag cgccactacc gcaccggcga gctcgcccgc
     2941 gccgccggcg ccggcccgca gatcgacgtc cgcgtcccgc tgctgttcaa cgacgacctg
     3001 atcgccggcg tcgccttccc gtcggccgac gacccggtct acgtcgccga cggcgacgcc
     3061 gatcagctga tctacgtcca ccagggcggc ggcgtcctgc gcagcctgct cggcgatgtc
     3121 cggttcgccc agggcgacta cgtgttcgtg ccgcgcggcc tgctccaccg cttcctgccc
     3181 tccgggccgc agcactggtt ctggttctcg ctgaccggcg gcctccacat ccccccccag
     3241 tggcgcaacc cgatcggcca gctccgcatg gacgccccct actcccaccg cgacttcaag
     3301 cggccgcagc tccagcctcc gctcgacgag ggcatccgcg atctgctcgt ccgccgcggc
     3361 ggcgcctggc acggcttcac gctcgaccac ccaccgctcg acgtcatcgg ctgggacggc
     3421 accgtctacc cctgggcgtt cccgatcctc gcgttccagc cccgcgtcag ctcggtccac
     3481 ctgccgccga cctggcacgg cacgttcgcc gcccgcggcg ccctgatctg cagcttcgtc
     3541 ccccgcccgg tcgacttcca ccccgaggcg atcccctgcc cgtatcccca cagctcgacc
     3601 cactgcgacg agatcatctt ctactgcgac ggcaacttca cctcgcgccg cggcgtcggc
     3661 ccgggcagcg tctcgcacca cccgatgggc gtgccgcacg gcccccatcc cggcagctac
     3721 gagaagtcga tcggcgccac cgctacctcc gagctcgccg tcatggccga cagcttcgcc
     3781 ccgctgcgcg cgacctcggc cgcgctcacc gtcgaggacg ccggctacca ggactcgttt
     3841 ctgtaagtga agagcatctc tgatcagcgc tacgcggcga cggaccacgc aagcgctcga
     3901 tcgtcgtccg gcgctggata cagcatcggg gtcgcaggtc gtccctgcga tccgggttta
     3961 cgcgatccgg cttgccgtcc atactgtgat cgccatgaac gatgagcgga cggccgtgca
     4021 gcgctggctg gcaggtcagc gagcgtcggc gcgcatgcag cgcgagctgc aagctgccga
     4081 agggccgcgc ccggcgcgtg ccgtggccca gtcgctggct gcgctggctg cgctggagac
     4141 catggggcga tggcctggtc cccgcgacgc cgtgagtgag cgcgcgatcg acgaggtgcg
     4201 gcgccgctgg gcccgcattc agcggcgtgc ccgggcagcg cgctcctgac cggtcccgga
     4261 tcgagagtgc gctgtccgcg ctgagcggag cgctctccga ggtcggggca ccctggatgg
     4321 tgatcggcgg aatcgccgtg atcgcccacg gcgtgcaacg tatgacgacg gacatcgacg
     4381 cggtcatcca gggcgatgcc gtatccgttc acacgctgat cgatgtgctg cagcgccatc
     4441 acatcgtgcc tcgcatcgct gatgcggagg cgttcgcggc tacgaacctg gtgctgctga
     4501 ccaggcatca gcccacggag gtcgatctcg acctttcctt cggctggacc gtgtttgagc
     4561 acgaagccct cgctgcacgc tccaggatgc gctacggcga agtcgcggcc ccgatggcgg
     4621 gtgtcgagga gctgctggtc ttcaaggcga tggccgggcg tccacgcgac atcgaagacg
     4681 ccgtcacctt gctcacgctc tatcctcgca tcgacctgcg ctctgtgcgt actcgcgtcg
     4741 ccacgctcgc cgagctcgcc gaggcgcccg aattgatgct ggggttcgag cggatcgttc
     4801 gcgccgccgc gatgcccaca aagcacacgt caccagcgaa gccgccagcg cgccgcgtgc
     4861 gaccaggagt cgtcgaacgg accacgccac gaccagcgcg tccacgcgca gccagcggcc
     4921 tcggcaagaa gcggaggcgt tctccacgct gaggtagctg cgaacggcag tgggcgcgcc
     4981 tgggggggtc atccgctcgg gctgagagca ggcgttcctg cgtgagcacc aaacacgagg
     5041 gcgtcgtcgc gcgccgccag aagagacatg attcgcctgc gccttggcgg atcgctgccg
     5101 tgatacggtg ccggccgatc catgcggacg acccgggcga tccagatcgt gtcgtgtcac
     5161 gccgagggcg aggtcggcga cgtgatcgtc ggcggggtgc cgccgccgcc cggcgacacg
     5221 ctgtgggagc agtcgcggtt catcgccacc gaccagacgc tgcgcaactt cgtgctcaac
     5281 gagccgcgcg gcggcgtgtt ccgccacgtc aacctgctgg tgccgccccg ggacccgcgg
     5341 gcgcagatgg gcttcatcat catggagccc gaggacacgc cgccgatgtc cggatcgaac
     5401 tcgatctgcg tggcgaccgt gctgctcgac tgcgggatcc tggcgatgac cgagcccgag
     5461 acccgcctgg tgctcgaggc gcccggcggc ctggtcgagg tcgtcgcgca ctgccggagc
     5521 ggcaaggccg agcggatcac cgtgcgcaac gtggcgtcgt tcgccgaccg gctcgacgcg
     5581 acgatcgagg tcgccgggct gggcacgctg accgccgaca ccgcgtacgg cggcgacagc
     5641 ttcgtgatcg tcgacgcgca ccgcctcggc ttccggctcg cgcccgacga ggcgcgcgat
     5701 ctcgccgaga ccggcgtgcg gatcacgcgc gccgccaacg agcagctcgg cttcgtccac
     5761 ccgaccaacc cgggctggaa ccacatctcg ttctgccagc tcgccgcgcc ggtcgcggcc
     5821 ctggccggcg tgcggaccgc ggccaacgcg gtcgtcgtgc agcccggcaa gatcgaccgc
     5881 tcgccgaccg ggaccggcgc ctcggcgcgg atggcggtga tgcgggcgcg gggcgagatg
     5941 caggtcggcg accgctacct ggcgcgctcg atcatcggct cggagttcct cggcaggatc
     6001 gaggccgacg cggaggtcgg cggccggccg gcgatcatcc cgtcgatctc cggccgtgcg
     6061 tggatcaccg gcacgcggca ggacatgctc gaccccgacg atccgtggcc ggcgggctac
     6121 cggctgtcgg acacctggcc gcgcatcacg cgctgacgcc cgccgcggtc cgactcccgc
     6181 tcgcgtgtgc gggccgcccg gagtacggtc cggccatggc gcgcaagaat catcgtttca
     6241 cactcgcggc ccggccgacc gggctgccca gaccgaccga cttcgcgttc gaggaggagg
     6301 cggtgcgcga gctcggcgac cgcgagctgc tggtcaaggt ccagtacatc tcgctcgacc
     6361 ccgcgatgcg cggctggatg aacgagggca agtcgtacat ccggccggtc gcgatcggcg
     6421 aggtgatgcg cgccggcgcg atcggcagcg tgatcgagtc gaagcatccc gggttcgcgg
     6481 ccggcacgca ggtcatcggc acgttcggcc tgcaggagta cgcgatctcg aacggcgagg
     6541 gcgtcacgcc gatcgatccg cggctgtcgc ccgggctggt gccgctgccg gtgatgctca
     6601 gcacgctcgg catgccgggg ctgacggcat acttcggcct gctggacatc ggcaagccgg
     6661 tcgccggcaa cacggtcgtg gtgtcgggcg cggccggcgc ggtgggcacc gtggtcggcc
     6721 agatcgccaa gatccacggc tgccgcgcgg tcggcatcgc cggcgg
//
LOCUS       DASVOR010001798         5921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_486384,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001798 DASVOR010000000
VERSION     DASVOR010001798.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486384"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..1921)
                     /locus_tag="VF516_33460"
     CDS             complement(242..1921)
                     /locus_tag="VF516_33460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112703.1"
                     /translation="MTRALALICCAASFLVSPAASAKPSRVALTQIEGDTSGDVHDAV
                     AEALEGKELSLIASREVNRAVDKLGDLSDLTEKDFKKLATELDADAIVAGKLDKVGSA
                     KTLKFRLFVHKKMAKGFTVSFKDAKSEKFRTLLHDKILDKIGVTGAGPGGDDDEDARP
                     AKKKKAADDEEDPLAKKDKKARKGKDAKADADDDDARPAKKTRAAKADDDDARPAKKT
                     ADADDDARPAKKKTDADDDARTAGGDDDAKPAKKKADADDDDAAPRKAKKRVAAADDG
                     AEVEATAAPIAESARGANRAGIRLDIGGSVLQHSFKFNTVQLPQRPRDTVLSPVPGAR
                     LDGEVYPLALTGSQGAAAGLGLGFEYDRTLSLHLNPTDNTPGANPMTAKVGVKQGHYT
                     IGLRYRLVFGKTETSPTLTLGAGYGKQLFSVNLADAGNSDALRNTLKQNTPETEYTIF
                     DPGAMFRVPVTRMVAFALGGRAMIITSAGPIQTVSSYGRARVYGAEGVIALDVVLGQH
                     LGLRFSGEFSQIGYTFLGTGALTDVDGNKMQDVGGLADRMIGGAATLTLLY"
     gene            2045..2548
                     /locus_tag="VF516_33465"
     CDS             2045..2548
                     /locus_tag="VF516_33465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112704.1"
                     /translation="MASSTRMDPVDRHNLTITGRHRAVTGPHVTVEDDVLVIVTQAFG
                     PRGDNLVGISDVQFDGHPAVTIGIRALGREGLVHLSPIHGDGRKTKPFDIPAGTRCEL
                     FCPVSRQPLDLVGDVEDGSGARYYAIYLTPQLSHGSMVMVSDVWGHYHSRIIDDFELI
                     SAWATRQ"
     gene            complement(2684..3823)
                     /locus_tag="VF516_33470"
     CDS             complement(2684..3823)
                     /locus_tag="VF516_33470"
                     /inference="COORDINATES: protein motif:HMM:NF019140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX8112705.1"
                     /translation="MATPATEAEWIAAATGKNLRQIEELVADHHPGDRPDDPPDPKAR
                     RHVVRFELSAETFALLRQTRTVLDDEHGTNLPEDAFIAALCNAVLDDAPQAEPTGRAK
                     FQIAMTVCERCRQGWQEGAGAQIAVAPAAVDRAMCDAQHIGSIDGVEPGRAYQDILPA
                     VARLVWRRDGGRCRVPGCRSSRGLEIHHLIHRADGGTHEASNLALVCAACHQAHHDGR
                     LTISGRVDRIEIHRPSPHVGAREAVTSAIRPGAVADATDAALPVVRPGAHVNATDTAT
                     PAARPSVHADARDAATPAARPGAHRDAMDAATPAACLGAHVGAADKLDVAIVRTQAKA
                     ALIGLGWKPAIAQAAVAAAAAATGTEMALERFIIEALRRCPVPRA"
     gene            4968..5279
                     /locus_tag="VF516_33475"
     CDS             4968..5279
                     /locus_tag="VF516_33475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112706.1"
                     /translation="MKNHRSTQSRVSKDAENDNFDAGTALENLRAEVIDIEALARATE
                     ATSEALPAPATDHQRVVYGRIQSLATKASEQASASLRFANAQVAALTAQMESRRKAAE
                     R"
     gene            complement(5437..>5921)
                     /locus_tag="VF516_33480"
     CDS             complement(5437..>5921)
                     /locus_tag="VF516_33480"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112707.1"
                     /translation="TPRPTPVLAVLGLARPSPPLFPDAAPLVIGCDRDLAVLAAARNN
                     ARAAGVADEVIWQRTDIARLTPGLVDDIARERGRAPTGTGVLLTNPPYGERLDDADLG
                     ELYAAIAETCRRFTGWRAGFLVGSPELERVFQRAIGRPRIKKPLANANLRAYFYLYDL
                     "
BASE COUNT          858 a         2083 c         2037 g          943 t
ORIGIN      
        1 ggtcagctcc agcatcgcga ggttcgccgc gcaccgcagg taggcggtga tcagaggaat
       61 tccgagctgg aagtaggcgc cgtgctcgcg cggcaggagc gatcgccggg tcatgccatg
      121 gggtcgagca accggcacgc ctgccctacc ggccgatcgc cgcgccggcc gcgcaccgcc
      181 tgcacggcgc cgccggatcg cctgcagttc ctgcgcaaac cggcgcgccg ccgctgcgcg
      241 gtcagtacag cagggtgagc gtcgccgcgc cgccgatcat ccgatccgcg aggccaccga
      301 cgtcctgcat cttgttgccg tcgacgtcgg tcagcgcgcc ggtgccgagg aacgtgtacc
      361 cgatctggct gaactcgccg gagaaccgca gcccgaggtg ctggccgagc acgacgtcga
      421 gcgcgatgac gccctcggcg ccgtagaccc gggcgcggcc gtacgagctc accgtctgga
      481 tcggccccgc gctggtgatg atcatcgccc gaccgccgag cgcgaacgcg accatccggg
      541 tcaccggcac ccggaacatc gcgccgggat cgaagatcgt gtactcggtc tccggcgtgt
      601 tttgcttgag cgtgttgcgc agagcatcgc tgttcccggc gtccgcgagg ttgaccgaga
      661 acagctgctt gccgtagccg gcgccgagcg tgagcgtcgg gctggtctcg gtcttgccga
      721 acaccagccg gtagcgcagc ccgatcgtgt aatggccctg cttgaccccc accttggccg
      781 tcatcgggtt ggcgccgggg gtgttgtcgg tggggttgag gtgaaggctg agggtccggt
      841 cgtactcgaa gccgagcccc aggccggccg ccgcgccctg cgagccggtg agcgccagcg
      901 ggtagacctc gccatcgagc cgggcccccg ggaccgggga cagcacggtg tcccggggcc
      961 gctgcgggag ctgcacggtg ttgaacttga agctgtgctg cagcaccgag cccccgatgt
     1021 cgagccggat cccggcccgg ttggcgccgc gggccgactc ggcgatcggc gcggcggtcg
     1081 cctcgacctc ggcgccgtcg tcggcggccg cgacccgttt cttggccttg cgcggcgccg
     1141 cgtcgtcgtc gtccgcgtcc gccttcttct tcgccggctt ggcgtcgtcg tccccgcccg
     1201 ccgtccgcgc gtcgtcgtcc gcgtccgtct tcttcttggc cggcctggcg tcgtcgtccg
     1261 cgtccgccgt cttcttggcc ggcctggcgt cgtcgtcatc ggccttggcc gcgcgggtct
     1321 tcttggccgg ccgcgcgtcg tcgtcgtccg cgtcggcctt ggcgtccttg cccttcctgg
     1381 ccttcttgtc cttcttggcg agcgggtcct cctcgtcgtc cgccgccttc ttcttcttgg
     1441 ccggccgcgc gtcctcgtcg tcgtcgccgc ccgggccggc gccggtgacg ccgatcttgt
     1501 cgaggatctt gtcgtgcagc agcgtgcgga acttctcgga cttggcgtcc ttgaagctga
     1561 ccgtgaaccc cttggccatc ttcttgtgga cgaacagccg gaacttgagc gtcttggcgc
     1621 tgccgacctt gtcgagcttg ccggcgacga tcgcgtcggc gtcgagctcg gtcgcgagct
     1681 tcttgaagtc cttctccgtc agatccgaga ggtccccgag cttgtcgacc gcgcggttga
     1741 cctcgcggct ggcgatcagc gacagctcct tgccctcgag ggcctcggcg accgcgtcgt
     1801 gcacgtcgcc ggaggtgtcg ccttcgatct gggtcagcgc gacccggctg ggcttggccg
     1861 acgcggcggg agataccagg aagctagcgg cgcagcatat caacgccagg gctcgggtca
     1921 ttcatgtctc cgcgatgcca gctcagtccc gggatgagct gggagcaacg agtatacacg
     1981 cagatgacac gagccacccg gtttgcggtg tcgctcacac accgcttgcc aagtgccttg
     2041 agccatggcc agctcaacgc gcatggaccc cgtcgatcgc cacaacctca ccatcaccgg
     2101 ccgtcaccgc gcggtcaccg ggccgcatgt caccgtcgag gatgatgtcc tggtgatcgt
     2161 gacgcaggca ttcgggccgc gcggcgacaa cctcgtcggc atctccgatg tccagttcga
     2221 cggccacccc gccgtcacga tcgggatccg cgcgctcggc agggagggcc tggtccacct
     2281 gtcgccgatc cacggcgacg ggcgcaagac caagccgttc gacatcccgg cgggcacgcg
     2341 ctgcgagctg ttctgtccgg tgtcgcgcca gccgctcgac ctggtcggcg acgtcgagga
     2401 tggctcgggc gcgcgctact acgcgatcta cctcacaccc cagctgtccc acggctcgat
     2461 ggtgatggtc agcgatgtgt ggggccacta ccactcgcgc atcatcgacg acttcgagct
     2521 gatctcggcg tgggcgacgc ggcagtgatc acagatcacg atgaccccgc gtggtgtcgt
     2581 ccgcgccgct gcccgcgctc gcccggctgt gcggcgtcgc acacgatctt cggcgagttg
     2641 gcggccgcgc gccctcgcta acatccgcgg ctgctcctcg ggctcacgct cggggtacag
     2701 gacagcgccg cagcgcctcg atgatgaaac gctcgagcgc catctccgtg ccggtggcgg
     2761 cagccgccgc cgcaacggcc gcctgtgcaa tggcaggctt ccatcccagg ccgatcagcg
     2821 ccgccttcgc ctgggtccgg acgatggcca catcgagctt gtcggccgcg cccacgtggg
     2881 cgccgaggca tgcggctggc gtcgcggcgt ccatcgcgtc cctgtgggca cccgggcgag
     2941 cagctggcgt cgcggcgtcc cttgcgtccg cgtgaacgct gggacgcgcg gccggcgtcg
     3001 cggtgtccgt cgcgttcacg tgggcaccgg ggcgtacgac tggcagtgct gcgtccgtcg
     3061 cgtccgcgac ggcaccaggg cgaatggccg acgtcactgc ttcccgcgcg cccacgtggg
     3121 gactcgggcg atggatctcg atccgatcga cccgacccga gatcgtcagc ctgccgtcgt
     3181 ggtgggcctg gtggcaagcg gcacacacca gggcgaggtt cgacgcctcg tgcgtgcccc
     3241 catccgcgcg atggatcaga tgatggatct ccagcccgcg ggacgagcgg cagcccggca
     3301 cccggcaacg gccaccatcg cggcgccaca ccaggcgcgc gacggcgggc agaatgtcct
     3361 ggtacgcccg ccccggctcg acaccatcga tcgatccaat gtgctgggca tcgcacatcg
     3421 cgcgatcgac cgctgcgggg gcgacggcga tctgcgcacc ggctccctcc tgccagccct
     3481 gacgacagcg ctcacacacc gtcatggcga tctggaactt cgcgcggccg gtgggctcgg
     3541 cctgcggtgc gtcgtcgagc acggcgttgc acagcgcggc gatgaacgca tcctcgggca
     3601 ggttcgtgcc gtgctcgtcg tcgagcaccg tacgggtctg acgcagcagt gcgaacgtct
     3661 cggccgagag ctcgaaccgg accacgtgcc tgcgcgcctt cggatccggt ggatcgtcgg
     3721 ggcgatcgcc cggatgatga tcggcgacga gctcttcgat ctggcgcagg ttcttgccgg
     3781 tcgcggcggc gatccactcc gcctcggtcg cgggcgtcgc cacccgggtc agctcgcgga
     3841 tggcagaaaa cggcagctcc ccgccggcga gcgcagccgt gagctcgggc aagtcgccga
     3901 gcgcacgggc gacccgtagc cggtcctggg cgctacgtgg cgcatagccg agcacgcgct
     3961 cgaggtaatc cagtgccgag accatgccca gcgggcgcca gatctccagc gcctcggcgt
     4021 cgcgcagcca gcgcgcctcg tcggcatcga gcatagagcg gcgcagcgcg atcgcgcgca
     4081 gggctcgatc caccgcgcac cagcggggct cgccggggca tggcaactcg aacgcccccg
     4141 cgaccgccgg atcgaccgcg acatctgatt cctcgacggc accgcattgc atgacccatc
     4201 tcctgtcgtt gtcgtgctcc ttgtagccac acggcatggg atgtcaatgg cacatgtgcg
     4261 aagaaaagcg atctcttctt gagatcggag tcaacgatcg ccgtacatgg cattctgtac
     4321 gatggaaaac aagatgcctc attcatcaac tcaattggta atcatcgata cgcatgaagc
     4381 catggtggcg atccatcgga cgggcgcagc gcacacgacg ccagcgcaga ggagggctcg
     4441 caagactcgc aggcgctggg tgcgccgtgt tggctcggcg tgttcccgct gcgtggagcg
     4501 cgccatcgag cgattcctcg tcggggggat cgaaatctga tccgcctcgg ctcgtggatg
     4561 ccacgcggtg gagcccctcg aacgaagcct tcgagagagc gccgtacggc ctgtggacaa
     4621 cgggtcggcc tgatgacccg cgccgcggcc aaccggatcg ataccggcgc ggctcacagg
     4681 cctccgccgt tccccacagt ccttgccctc gagccgatgc tgccgatgag agtatatata
     4741 tatttaattg caggatcgag gccagagcac aaggcgacgc gtccagcgta tcgaggtgat
     4801 tgttccacga gtcgatcaat cactctcaca accgccgatt gactgcttgc cgtgctcccg
     4861 caagataggt gcgttccatc gggaacgagg cagcgccgcc atcggcgcaa gcagtctgca
     4921 caaagcggcc cgccggtgca cgaacaccgg cgagcccagg agcgacgatg aagaaccatc
     4981 gcagcaccca gtccagggta tccaaagacg ccgagaacga caacttcgac gcggggaccg
     5041 cgctcgagaa cctccgcgcg gaggtcatcg acatcgaggc gctcgcccgc gccacggagg
     5101 cgacctccga ggcgcttccc gcgccggcga ccgatcacca gcgcgtggtg tacggccgga
     5161 tccagtcgct cgccaccaag gcatcggagc aggcgagcgc ctcgctccgc ttcgcgaacg
     5221 cgcaggtcgc cgcgctcacc gcgcagatgg agtcacgccg gaaggcggcg gagcgctgaa
     5281 cgtggcggct cataccgccg cgcgatggcc ggcgcggcgg gtgcgcccac gtgggcgcgc
     5341 gccaagaagc gatcatggca accatctcca gtcgctccat cgtcgcagcc gatcggttcg
     5401 ctctgatctc ggcgtgggcc acgcgccagt gaccggtcac agatcgtaga gatagaagta
     5461 ggcgcgcagg ttggcattgg cgagcggctt cttgatccgc ggccggccga tcgcgcgctg
     5521 gaacacgcgc tcgagctcgg ggctgccgac caggaacccg gcgcgccagc cggtgaaccg
     5581 ccggcaggtc tcggcgatcg cggcgtagag ctcgccgagg tcggcgtcat cgagccgctc
     5641 gccgtacggc gggttggtga gcagcacgcc ggtcccggtc ggggcgcggc cgcgctcgcg
     5701 cgcgatgtcg tcgaccaggc cgggcgtgag ccgggcgatg tcggtgcgct gccagatcac
     5761 ctcgtcggcg actccggcgg cgcgggcgtt gttgcgcgcg gcggcgagca ccgcgaggtc
     5821 gcgatcgcag ccgatcacca gcggcgcggc gtcggggaac agcggcggcg acgggcgcgc
     5881 gaggcccagc accgcgagca ccggggtcgg ccgcggggtc g
//
LOCUS       DASVOR010001799         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_48173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001799 DASVOR010000000
VERSION     DASVOR010001799.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..186)
                     /locus_tag="VF516_33485"
     CDS             complement(<1..186)
                     /locus_tag="VF516_33485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112708.1"
                     /translation="MRAASKVVALSSLLVAASVAASPSPKDKPTDKTPAAKAGAKTGA
                     TAAPSGAPAGTAGTVSAD"
     gene            complement(392..1465)
                     /gene="obgE"
                     /locus_tag="VF516_33490"
     CDS             complement(392..1465)
                     /gene="obgE"
                     /locus_tag="VF516_33490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEX8112709.1"
                     /translation="MQFIDEAVIHVKAGDGGNGAAAFRREDGVPRGGPSGGDGGNGGS
                     VIVVADPRLASLLDYKFRRRYTADRGEDGRNKDQYGASGEDLVLRVPVGTVIAEISGE
                     VSGEVSGEVSEEVSENEDGGLLADLSEPGARFVIARGGTGGRGNIHFKTPWNQAPRTA
                     EPGTPGEERTVRLELKLLADVGLLGYPNVGKSTFIRRVSRARPKVADYPFTTLVPNLG
                     VVQLSDERQFVIADIPGLIEGASQGAGLGHQFLRHVERCRVLLHIVEATFTTGPDRTP
                     LRDFDVINSELAGYSPELAAKPQVVVLNKIDAMASDEVSMHRRAFADRGVELLTISAA
                     TGAGIDAVLERLWSHLHPSPREA"
     gene            1558..1680
                     /locus_tag="VF516_33495"
     CDS             1558..1680
                     /locus_tag="VF516_33495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112710.1"
                     /translation="MDRAVVVPASRRADAATLGATLVAAVAFGPTAMRPPPPPR"
BASE COUNT          260 a          690 c          656 g          308 t
ORIGIN      
        1 gtccgcgctc accgtcccgg ccgtcccggc gggcgctccc gacggcgcag cggtcgcgcc
       61 ggtcttggcc ccggccttcg cggcaggcgt cttgtcggtg ggcttgtcct tgggactcgg
      121 gctcgcggct acgctcgctg cgaccagcag cgatgacagc gcgacgacct tcgatgcagc
      181 tcgcatgcgt gttctccagc cggcccttgc cggcgacctc gtccgccgag tccaacctga
      241 aagcaccggg cgcgatgcgg cgctttgttc agtggaatgc agtagttgta gcatcgcgct
      301 ccgggcccct caagccggcg aggcgcgcct cgcgggcgaa cctgccgagc agtcgtcgag
      361 acgtcaaccg agacgtagat cgatggtcga ctcatgcttc cctcgggctc gggtggagat
      421 gagaccacag gcgctctagc acggcgtcga ttcccgcgcc ggtggccgcg gagattgtga
      481 gcaactcgac accgcggtct gcgaacgcac gtcgatgcat gctgacctcg tcagatgcca
      541 tggcgtcgat cttgttcaac acgaccacct gcggcttggc cgcaagctcc ggcgagtagc
      601 ccgcaagctc ggaattgatc acgtcgaaat cgcgcagcgg tgtgcgatcg ggcccggtgg
      661 tgaaggttgc ctcgacaatg tgaagtagga cacggcagcg ctcgacgtgc cgcaggaact
      721 ggtgtcccaa tcctgcaccc tgcgaagccc cttcgatgag accggggatg tcggcgatga
      781 caaactggcg ctcgtcgctc agctggacca cgccgaggtt gggcaccagc gtggtgaagg
      841 ggtaatccgc caccttgggc cgcgcgcgcg acacgcggcg gatgaacgtc gacttgccga
      901 cgttggggta cccgagcagc ccgacgtccg cgagcagctt gagctccagc cgcaccgtgc
      961 gctcctcgcc cggcgtgcca ggttctgccg ttcgaggtgc ctggttccac ggcgtcttga
     1021 agtggatgtt gccgcgaccg cccgtgccgc cccgcgcgat cacgaaccgc gctcccggct
     1081 cggacaggtc ggcgagcagg ccgccgtcct cattctcgga gacctcctcg gagacctccc
     1141 cggagacctc cccggagacc tccccggaga tctcggcgat caccgtgccc acgggcacac
     1201 gcagcacgag gtcctcgccc gaggcgccgt actggtcctt gttgcggccg tcttcgccgc
     1261 ggtccgcggt gtagcggcgg cggaacttgt aatcgagcag cgaagccagc cgcggatcgg
     1321 ccacgacgat gacgctgccg ccgttcccgc cgtcgccgcc cgacggaccg ccgcgtggta
     1381 caccatcctc gcgccggaac gcggccgcgc cgttgccgcc gtcgccggcc ttcacgtgga
     1441 tgacggcctc gtcgatgaac tgcatggcgg tccgatgaca gatatggccc cgtccgcggg
     1501 cgcggggcaa cccgccgggc ccacgaaccg ggctcgtccc atgcggtgga tcaccgcgtg
     1561 gaccgtgccg tggtggtgcc ggcgtcgcgg cgcgccgatg ccgcgaccct gggcgcgacg
     1621 ctcgtcgcgg cggtcgcgtt cggcccgacc gcgatgcggc cgccgccgcc gccacgctga
     1681 tcatcctcca actcccctgg gtcgtgctct ggttcgcggg cgagcgcgcg cggcgccctc
     1741 gtcgtgctcg ccctaggaac gtgcagcgca tggcgccctc gggtcggtag atatggcctc
     1801 gcccaccgct tcggggcaac tcgttgccca ccccgaattg cgctggatcg cggtttggag
     1861 ccgcgcggcg ctggtcccgg cctggcggcg cgccctcggc gcctgggtcg gctg
//
LOCUS       DASVOR010001800         5762 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_48202,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001800 DASVOR010000000
VERSION     DASVOR010001800.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48202"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..851)
                     /locus_tag="VF516_33500"
     CDS             complement(147..851)
                     /locus_tag="VF516_33500"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8112711.1"
                     /translation="MPTADRPAFIPLSVRSFLAQDWPNRELLVIDDGRDAVGDLVAGV
                     PGVRYLRLSRRASIGAKRNLACAEARGELIVHWDDDDWYAPSRLTWQATPIAAGRADV
                     TGLENRFTLQLPAGQFWTVEREPHQPMVRGDVHNGTLMFRRSIFAEGVRYPEVNLAEG
                     AAFLREAMDRGHRLARLENPDVFVYVRHGGNTWRFAPGQFLNRRRRERVARPSSLSAE
                     TLEAYRIAAADPTRRT"
     gene            complement(969..4055)
                     /locus_tag="VF516_33505"
     CDS             complement(969..4055)
                     /locus_tag="VF516_33505"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112712.1"
                     /translation="MKKTVLMVALIAAPASAFADELALDSDALGNLEARSIGPAAMSG
                     RVAAIDAVQGEKLTLWVGGASGGVWKSVDGGITWKATFDKHTQSIGAVAIDSTNPETV
                     WVGTGESWLRNSVSVGDGIYKTTDGGDSWQRVGLPDSERVARIQIDPRKHDTVYVCAT
                     GHAWNANAERGVFRTTDGGRTWQKVLYVDDNTGCADLEIDSHDPSTLWAAMWQYRRTP
                     WSFTSGGAGSGLYKTTDGGAHWRQVRAGLPDGQLGRIALAAANSKAGVIYAYVESRRT
                     GVFRSADGGEHWTEANTGPSLAARPFYFGRMVVDPGNENRIYKPSLFLSVSEDGGKTF
                     AAASGSFHGDVHAVWVNPRDSAQVWIGTDGGVYGSTDRANHWRFVGGLPLSQFYHVSV
                     DDETPFHVYGGLQDNGSWMAPSRRSGGIGNRHWQNVGIGDGFWTFPDPVDKDVVYSEY
                     QGGKLLRVNKKTLETKQIEPFARAGEAKYRFNWNTPIHLSPTRPGVMYVGAQFLLRSA
                     DRGDTWTRISPDLTTNDASKLQQEESGGLSVDNSSAENHCTIFAISESPKNANVIWVG
                     TDDGNVQVSRDGGKSWTNVVANVPGLPKATWVSHVEASRFDPAAALATFDGHATGDMT
                     TYVYRTTDYGKTWQPLATPELHGYAHVIKEDTVDRDLLFLGTERGLWISLDGGKHWAE
                     FKGGGDGKFPPVAVRDLVIHPSTGDLVLATHGRGIWIVDDISSLRKLTPAVLAQDFAF
                     LDTRPSVLSIPAQEQGFEGDEGYDGQSPPEAAVFTYYLKKRHIFGDLTIEILDKTGKV
                     LTSFPGGKRRGINRVTWPMRLPPPKLPPAATEVQSIYSFLGPRVPDGTYTVRIKKGDK
                     TYTTPLKIVQDPRSTHSAADRALQQKTVHELYGMLNDLTYLVDALADLKAQAADRSAH
                     AKDARKSTDDLAAALEALRSKLVATREVGFISGEEQLRERLGECYGGVNGYDGRPTES
                     QLHRKDVLKKELADAATELDGIVKAHLEAVNAALARDKLPPITKLSRDDWQNKQTN"
     gene            complement(4058..4807)
                     /locus_tag="VF516_33510"
     CDS             complement(4058..4807)
                     /locus_tag="VF516_33510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112713.1"
                     /translation="MLVVATSASARAESVDEIVAKYLQARGGADKVRGVQSLRMTGKL
                     VAGPGIEAPFTIEVKKPGRMRFEVVIQGMTIVQAVDGDSGWTIQPMTGKKDAERMSAD
                     DLKEAQDQADIDGPLVDYKKKGHKVELAGKEKIEGTDAYKLKVTKKNGDIEYVYLDAD
                     AFLEIKIEGKRMIRGTPVEFESTSGDYKEVSGVMFPFSAEFGNKGQKVKLSFTKIEVN
                     PKLDDARFKMPAPAPAPAPDTKPTPPPAKGK"
     gene            complement(5104..5562)
                     /locus_tag="VF516_33515"
     CDS             complement(5104..5562)
                     /locus_tag="VF516_33515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112714.1"
                     /translation="MMIGGDKPAPRDPSAPAPKLERDYFIEAARRIGVVIDVRTGGEA
                     WLDKRVTDGAYDIAELVWTGMVDGDPAQVLAHAARSPRIDRALDALAAAWDPAEHAKL
                     APDLATALAETWPIAGIVADAPQGLIAKRVQGVRVWDGWLDLSQLSFTGP"
BASE COUNT          815 a         2029 c         1925 g          993 t
ORIGIN      
        1 agcgtcgtgt cgagcagcgc gcgcaggcgc ggggtcgccg gggggctgga tgcgggcgtg
       61 accacgcgcc tagcctaccc cgcccgggcg cagcggggat cattcacccc gccgccggtt
      121 gcgaccgtcg cagcgccggc atctcgtcat gtccgacgcg tcgggtcggc ggctgcgatg
      181 cgataggcct cgagcgtttc ggccgagagt gacgagggtc ttgccaccct ctcccgcctc
      241 cgccggttga gaaattgccc cggcgcgaat cgccaggtat tgccgccgtg gcggacgtag
      301 acgaacacgt ccggattctc gagcctggcc agccggtgcc cgcggtccat ggcctcgcgg
      361 aggaacgccg cgccctcggc caggttgacc tcggggtatc gcaccccctc ggcgaagatc
      421 gagcggcgaa acatcaacgt gccgttgtgc acgtcgccac ggaccatcgg ctggtgcggc
      481 tcgcgttcga cggtccagaa ctggcccgcc gggagctgga gcgtgaaccg gttctccagc
      541 ccggtgacat cggcgcgccc ggcggcgatc ggtgtcgcct gccaggtcag acgagacggc
      601 gcgtaccagt cgtcgtcgtc ccagtgcacg atcagctcgc cgcgggcctc cgcgcaggcg
      661 aggttgcgct tggcgccgat cgaggcgcgc cgggacagcc gcaggtagcg tacacccggc
      721 actccggcga ccagatcgcc cacggcatcg cggccgtcgt cgatgacgag gagctcgcgg
      781 ttcggccagt cctgcgccag gaagctgcgc acgctcagcg ggatgaacgc cggccggtcg
      841 gcggttggca tgatgcacga gatcaggggg cggtcgccag cgacgacggc cggctgtgcg
      901 gtgaccgcgg acgaagcgga cgcggacgag ggaggcggcg gcgggggcat gtcggtcacg
      961 cccgccgcct agttggtctg cttgttctgc cagtcgtcgc gggacagctt cgtgatcggc
     1021 ggcagcttgt cgcgcgccag cgcggcgttg acggcctcga ggtgggcctt cacgatgcca
     1081 tcgagctcgg tggccgcatc ggccagctcc ttcttgagca cgtccttgcg atgcagctgc
     1141 gattcggtgg ggcggccgtc gtacccgttg acgccgccgt agcactcacc gagccgctcg
     1201 cgcagctgct cctcgcccga gatgaacccg acctccctgg tcgcgaccag cttgctgcgc
     1261 agggcctcga gcgccgccgc gaggtcatcg gttgacttcc gggcgtcctt ggcgtgcgcc
     1321 gagcgatcgg cggcctgcgc cttcaagtcg gcgagcgcgt cgacgagata cgtcagatcg
     1381 ttcagcatgc cgtagagctc gtgcaccgtc ttctgctgca gcgcccggtc ggcggccgaa
     1441 tgcgtgctgc gcgggtcctg cacgatcttg agcggcgtcg tgtacgtctt gtcgcccttc
     1501 ttgatgcgca ccgtgtaggt gccgtcggga acccgcgggc ccaggaacga gtagatcgac
     1561 tggacctcgg tggccgcggg cggcagcttc ggcggtggca gccgcatcgg ccacgtcacg
     1621 cgattgatgc cgcgccgctt gccgcccgga aagctcgtca gcaccttgcc ggtcttgtcg
     1681 aggatctcga tggtgaggtc gccgaagatg tgccgcttct tcaggtagta cgtgaacacc
     1741 gccgcttccg gcggcgattg gccgtcgtag ccttcgtcgc cttcgaagcc ctgctcctgg
     1801 gccgggatcg acagcaccga gggacgcgta tcgaggaacg cgaagtcctg cgccagcacc
     1861 gcgggcgtca gcttgcgcag cgagctgatg tcgtcgacga tccagatgcc gcgcccgtgc
     1921 gtcgccagca ccaggtcgcc ggtggacgga tggatcacca gatcgcgcac ggcgaccggc
     1981 gggaacttcc cgtcgccgcc gcccttgaac tccgcccagt gcttgccgcc gtcgagcgag
     2041 atccacagcc cgcgctcggt acccaggaac agcagatcgc gatcgaccgt gtcttccttg
     2101 atcacgtgcg cgtagccgtg cagctccggc gtcgcgagtg gctgccacgt cttcccgtag
     2161 tcggtggtgc gatagacgta cgtggtcatg tcgccggtcg cgtgaccgtc gaacgtcgcc
     2221 agcgccgccg cagggtcgaa gcggctggcc tcgacgtgcg agacccacgt cgccttcggc
     2281 aagccgggaa cgttcgcgac gacgttggtc cacgacttgc cgccgtcgcg cgagacctgg
     2341 acgttaccgt cgtcggtgcc gacccagatc acgttcgcgt tcttcggcga ttcgctgatc
     2401 gcgaagatcg tgcagtggtt ctcggcgctc gagttgtcga ccgagagtcc gcccgactcc
     2461 tcctgctgca gcttcgaggc atcgttcgtc gtcagatccg gcgagatgcg cgtccaggtg
     2521 tcgccgcgat cggccgagcg caacaggaac tgtgcaccga cgtacatcac gccgggcctg
     2581 gtcggcgata gatgaatcgg cgtgttccag ttgaagcgat acttcgcctc gccggcgcgc
     2641 gcgaacggct cgatctgctt ggtctcgagc gtcttcttgt tcacgcgtag cagcttgccg
     2701 ccctggtact ccgagtagac gacgtccttg tcgacgggat cggggaacgt ccagaagccg
     2761 tcgccgatcc ccacgttctg ccagtggcga ttgccgatgc cgccggaccg ccgcgacggc
     2821 gccatccagc tgccgttgtc ctgcaggccg ccgtacacgt ggaacggagt ctcgtcgtcg
     2881 acgctcacgt gatagaactg cgacagcggc agcccaccga cgaagcgcca gtgattcgcg
     2941 cggtcggtcg agccgtagac accgccgtcg gtgccgatcc agacctgcgc gctgtcccgc
     3001 ggattcaccc acaccgcgtg cacgtcgccg tggaacgacc ccgacgccgc cgcgaacgtc
     3061 ttgccgccgt cctcgctgac cgacaggaac aggctcggct tgtagatgcg gttctcgttg
     3121 ccgggatcga ccaccatgcg gccgaagtaa aacggccgcg ccgccagcga cgggccggtg
     3181 ttcgcttcgg tccagtgctc gccgccgtcg gcggagcgaa acacgccggt gcgcctggac
     3241 tcgacgtacg cgtagatcac gccggccttg ctgttcgccg ccgcgagcgc gatccggccg
     3301 agctgcccgt cgggcaatcc agcgcgcacc tgcctccagt gcgcgccgcc gtcggtggtc
     3361 ttgtagaggc cgcttccggc gccgcccgac gtgaacgacc acggcgtgcg ccggtactgc
     3421 cacatggcgg cccacagcgt gctgggatca tgcgaatcga tctccagatc cgcgcacccg
     3481 gtgttgtcgt cgacgtacaa caccttctgc cacgtgcggc cgccgtcggt ggtccggaac
     3541 acgccgcgct cggcgttcgc gttccaggca tggccggtcg cgcacacgta caccgtgtcg
     3601 tgcttcctgg gatcgatctg gatgcgcgcg acgcgctccg aatccggcaa gcccacgcgc
     3661 tgccagctgt cgccgccgtc ggtggtcttg tagatcccat caccgaccga cacgctgttg
     3721 cgcaaccacg attcaccggt gcccacccag acggtctcgg gattcgtgga atcgatcgcg
     3781 accgcgccga tcgattgcgt gtgcttgtcg aacgtggcct tccaggtgat gccgccgtcg
     3841 accgacttcc acacgccgcc cgacgcaccg ccgacccaca gcgtgagctt ctcgccctgc
     3901 acggcatcga tggcggccac gcggccgctc atggccgcgg gtccaatcga tcgcgcctcc
     3961 aggttgccga gcgcgtcgga gtcgagggca agctcgtcgg caaacgccga cgcgggcgct
     4021 gcgatcagcg ccaccatcag cacggtcttc ttcatggtca cttgcccttg gccggcggcg
     4081 gagtcggctt cgtgtcgggc gcgggcgcgg gcgccggcgc cggcatcttg aagcgagcgt
     4141 cgtcgagctt cgggttcacc tcgatcttgg tgaagctcag cttgaccttc tggcccttgt
     4201 tgccgaactc ggccgagaac gggaacatca cgccgctcac ttccttgtaa tcaccgctgg
     4261 tcgattcgaa ctcgaccggc gtgccgcgta tcatccgctt gccttcgatc ttgatctcga
     4321 ggaaggcatc ggcgtcgaga tacacgtact cgatgtcgcc gttcttcttg gtcaccttga
     4381 gcttgtaggc gtcggtgcct tcgatcttct ccttgcccgc cagctcgacc ttgtgcccct
     4441 tcttcttgta gtcgaccagc ggtccgtcga tgtcggcctg atcctgcgct tccttcagat
     4501 cgtcggccga catccgttcg gcatccttct tccccgtcat cggctgaatg gtccagccgg
     4561 agtcgccgtc gaccgcctgc acgatcgtca tgccctggat gacgacctcg aaccgcatgc
     4621 gcccgggctt cttgacctcg atcgtgaacg gcgcctcgat gccgggcccc gccacgagct
     4681 tgccggtcat gcgcaggctc tgaacccccc ggaccttgtc ggctccacca cgggcctgga
     4741 gatacttcgc gacaatctcg tcgacggatt cggcgcgtgc gctcgcgctg gttgcgacga
     4801 cgagcatgac agcaacgatc aacctctgca tggatcctcc tgtgggcggc ggccggacac
     4861 tctctcggag cttgccgtcg agcaagcacc gcgccaacgg caggaagtaa caatcggtcg
     4921 cgcatcacga ccgaagcaaa gcgcgccgca ccgggtcgat cgccgcgtcg cggcgtcgct
     4981 gcgactgtcg catgcggcgt cgcgaccgcg ctcgggcggc ccgcgaggaa caggacagcg
     5041 ccgatccggg ccagccgctt cgtccgttcg tttcgtcgtg gtggatgatt ttcagccgcg
     5101 ggctcacggc ccggtgaacg acagctgcga cagatcgagc cagccgtccc acacccggac
     5161 gccctgcacc cgcttggcga tcaggccctg cggtgcgtcg gcgacgatcc cggcgatcgg
     5221 ccaggtctcg gcgagcgcgg tggcgagatc cggggcgagc ttggcgtgct cggccggatc
     5281 ccaggcggcg gccagcgcgt cgagcgcgcg gtcgatccgc ggcgagcgcg ccgcgtgggc
     5341 caggacctgc gccggatcgc cgtcgaccat gccggtccac accagctcgg cgatgtcgta
     5401 ggcgccgtcg gtgacccgct tgtcgagcca ggcctcgccg cccgtccgca cgtcgatcac
     5461 gacgccgatc cggcgcgccg cctcgatgaa gtagtcgcgc tcgagcttgg gcgccggggc
     5521 cgacggatcg cgcggcgccg gcttgtcgcc gccgatcatc atcagccgga gctgcttgcc
     5581 ggcgtggtcg cggatgccgt ccttgggctc gctgtcggtc cagacgctcg gcccgccaga
     5641 cgctcggacc cggagcgcct acctgcgtac cctctgccca cgtagcgcgt gtctcgggcg
     5701 ctcaccgagg gctgacatcg cgtccagcct cacgctcgtc caacggcccc gcgccttggc
     5761 tt
//
LOCUS       DASVOR010001801         2069 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_339721,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001801 DASVOR010000000
VERSION     DASVOR010001801.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339721"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VF516_33520"
     CDS             complement(<1..260)
                     /locus_tag="VF516_33520"
                     /inference="COORDINATES: protein motif:HMM:NF022995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoglobin domain-containing protein"
                     /protein_id="HEX8112715.1"
                     /translation="MTFLEELLAFVGFDEGDRARLRELHPRLAPRFGAIASRFYDAVF
                     ASPGAAAVLSGPAQVERLRISLVDWMSSGLLGPHDERFYEKRS"
     gene            complement(257..1669)
                     /locus_tag="VF516_33525"
     CDS             complement(257..1669)
                     /locus_tag="VF516_33525"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8112716.1"
                     /translation="MTRLGRILVVDDEANARTALAELLRDEGYEVETAADAFKALGKY
                     ESFAPHIVVTDLKMPGMDGIELVKKIRATDEVTGIIVMTAFGAVQSAIEAMRAGAAEY
                     LTKPLNFEELLVVLAKVLENQAVRREARQLRQRVRERVAPGNIVGISPPMQRVFEVVD
                     QVAPSRATVLITGESGTGKELVANAIHQRSPRASGPFIKLHCAALAESLLESELFGHE
                     KGSFTGAMARKDGRFQLAEGGTLFLDEIGEISPSIQVKLLRFLQEHEFERVGGTQTIR
                     VDVRVIAATNRNLVEEVAKGRFREDLYYRLNVVALEMPPLRERKSDIPALGKFFLDRY
                     TKENGKTLEGFAPETMELLMAYDWPGNVRELENAVERSVVLASGPLIEPRHLPPNVRP
                     RVTPTGMPIIPGATLRDLERYAILETLKATGGSTSKAAEMLGISVRTIQYRLHQYNEA
                     PRSDIDVVQKMVASAESATE"
     gene            complement(1817..>2069)
                     /locus_tag="VF516_33530"
     CDS             complement(1817..>2069)
                     /locus_tag="VF516_33530"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF013730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HEX8112717.1"
                     /translation="ARGELGGGPASRFVEVGVNTPLAECEARDPKGLYAQARRGAADQ
                     LPGVGAAYEPPMSPEVTASGGFDDTAIAAIERHLDARPA"
BASE COUNT          281 a          751 c          705 g          332 t
ORIGIN      
        1 gaccgcttct cgtagaatcg ctcgtcgtgc ggcccgagca ggccgctcga catccagtcg
       61 accagcgaga tccggagccg ctcgacctgg gccggccccg acagcaccgc cgcggcgccc
      121 gggctggcga acaccgcgtc gtagaaccgg ctcgcgatcg ccccgaaccg cggcgcgagc
      181 cgcgggtgga gctcgcgcag gcgtgcgcga tcgccctcgt cgaacccgac gaaggcgagc
      241 agctcctcga ggaacgtcac tcggtcgcgc tctccgcgct cgcgaccatc ttctggacga
      301 cgtcgatgtc cgagcgcggc gcctcgttgt actggtgcag ccggtactgg atcgtgcgga
      361 cgctgatccc gagcatctcg gcggccttcg aggtcgaccc gccggtcgcc ttcagggtct
      421 cgaggatcgc gtagcgctcc agatcgcgca gcgtggcacc ggggatgatc ggcatgccgg
      481 tcggcgtgac ccgcggccgg acgttgggcg gcaggtggcg gggctcgatc agcggtccgc
      541 tcgcgagcac gaccgagcgc tcgaccgcgt tctcgagctc gcgcacgttg cccggccagt
      601 cgtaggccat gagcagctcc atggtctcgg gcgcgaagcc ctcgagcgtc ttgccgttct
      661 ccttggtgta gcggtcgagg aagaacttgc cgagcgccgg gatgtcgctc ttgcgctcgc
      721 gcagcggcgg catctcgagc gcgacgacgt tgagccggta gtacaggtcc tcgcggaacc
      781 ggcccttggc gacctcctcg accaggttgc ggttggtcgc cgcgatcacg cggacgtcga
      841 cccggatcgt ctgggtcccg ccgacccgct cgaactcgtg ctcctggagg aagcgcagca
      901 gcttgacctg gatcgacggc gagatctcac cgatctcgtc gaggaacagc gtgccgccct
      961 ccgcgagctg gaaccggccg tccttgcgcg ccatcgcgcc ggtgaacgag cccttctcgt
     1021 ggccgaacag ctcgctctcg agcagcgact cggcgagcgc cgcgcagtgc agcttgatga
     1081 acggccccga cgcgcgcggc gagcgctggt ggatcgcgtt ggcgaccagc tccttgccgg
     1141 tgccgctctc gccggtgatc agcaccgtcg cgcggctcgg cgcgacctgg tcgacgacct
     1201 cgaacacgcg ctgcatcggc gggctgatgc cgacgatgtt gccgggcgcg acccgctcgc
     1261 gcacccgctg gcggagctgg cgcgcctcgc gccggaccgc ctggttctcc agcaccttgg
     1321 cgagcacgac cagcagctcc tcgaagttga gcggcttggt caggtactcg gcggcgccgg
     1381 cccgcatcgc ctcgatcgcc gactgcacgg caccgaacgc ggtcatcacg atgatcccgg
     1441 tgacctcgtc ggtcgcccgg atcttcttga ccagctcgat gccgtccatg ccgggcatct
     1501 tgaggtcggt caccacgatg tggggcgcaa aggactcgta cttgccgagc gccttgaagg
     1561 catcggctgc ggtctcgacc tcgtagcctt cgtcccgcag cagctctgcc agcgcagttc
     1621 ttgcgttcgc ttcgtcatca acgaccagga ttcgtcccaa gcgggtcatg gccggcactg
     1681 tagcgacgat cgtgccagca taatccgcat ggttgcgagc gacttcgtgc aaggcgtgcg
     1741 ttccccacgc taggtgcgca aaccgtgcgc gattctccgg atcggtcccg ggctggggga
     1801 tgccgccccc ggatcgctag gccggccggg catcgaggtg gcgctcgatc gccgcgatcg
     1861 ccgtgtcatc gaacccgccg ctcgcggtca cctcgggcga catcggcggc tcgtacgccg
     1921 ctcccacgcc gggcagctgg tccgccgcgc cgcgccgtgc ctgggcgtac agccccttgg
     1981 ggtcgcgcgc ctcgcactcg gcgagcggcg tgttcacccc gacctcgacg aaccggctcg
     2041 ccggcccgcc gccgagctcg ccgcgcgcg
//
LOCUS       DASVOR010001802         2164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_164549,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001802 DASVOR010000000
VERSION     DASVOR010001802.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164549"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..937)
                     /locus_tag="VF516_33535"
     CDS             complement(89..937)
                     /locus_tag="VF516_33535"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX8112718.1"
                     /translation="MTPISSKRVYEVWTQVIQDEELYEAMLEGTHARLEDRRLDAEAI
                     EILDRLRAEPGTRWNVENLRFRAALHVGDTVLSYMPRMMRMVTGGDDGWRQDLCYEYL
                     AHHRWQPLGHLQLSECERFAGFVRSRVMKRRPTPPHLETVIGYELAVVRLLRSTAAIP
                     ADAWPVHRKVSDEDLPAARPRRGPCQVMVELEVDLRPWITSGDPTRGELGPGPITLLV
                     HVPTLDERYQVKAISDGVRVVLERCDGERTVDELATALEAEYDLPTAGVHRLVRMLLD
                     ERILCA"
     gene            complement(934..1797)
                     /locus_tag="VF516_33540"
     CDS             complement(934..1797)
                     /locus_tag="VF516_33540"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEX8112719.1"
                     /translation="MNPTALELGPLGVDPSLHEYAELLCDQFTGPLDAGYTLDPYMRP
                     MLEQIAARHKLIAHGNYGNEFGFEPLDETAGVLRHIAAAHAMNSPWYADHMFFGFQAT
                     AYVWSSPLPFSRAEVERVAGRAAALQDRLKMPLLHENAFYYAPAPGTEIAEAEFIATM
                     VEKAQTHLLLDLHNVYANSRNFQGYDAWGFLRTVPLDRVLEIHLAGGQQSDGWYHDFH
                     SYSVPEPVWEMLGYVVPRAPNLRAVVLEVQGPAHSHVSRAVDTSWVPMINNDLKRARA
                     VVDAHRTPATR"
     gene            complement(1884..>2164)
                     /locus_tag="VF516_33545"
     CDS             complement(1884..>2164)
                     /locus_tag="VF516_33545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112720.1"
                     /translation="ERFIQARAWARSAGLPRRLFVKVPEETKPLFLDLASPTFVELAA
                     RMVRKAQAVSISEMLPDLDELWLCDAEHRRYTSELRLVAVDPEPWLPG"
BASE COUNT          329 a          741 c          767 g          327 t
ORIGIN      
        1 gaatgcaaac ccggcgatcc ggatcaggta ggccggcagg atcgaccagc gagtcaaggg
       61 gacctccacc gcggggcgcc cccgtatctc aggcgcacag gattcgctca tcgagcagca
      121 tccgtaccag ccggtgcacg cccgccgtgg gcaggtcgta ctcggcctcg agtgcggtgg
      181 cgagctcgtc gaccgtgcgc tcgccgtcgc agcgctcgag cacgacgcgc acgccgtccg
      241 agatcgcctt gacctgatac cgctcgtcga gcgtcggcac gtggacgagc agcgtgatcg
      301 gcccggggcc gagctctccg cgcgtcggat cgcccgaggt gatccacggc cgtagatcga
      361 cctcgagctc gaccatgacc tggcacggtc cgcgccgggg ccgcgccgcg ggcaggtcct
      421 cgtcgctgac cttgcggtgg accggccacg cgtccgccgg gatcgccgcg gtggatcgca
      481 gcagacggac cacggccagc tcgtacccga tcacggtctc gaggtgaggc ggcgtgggac
      541 gacgcttcat cacgcgcgac cggacgaagc cggcgaaccg ctcgcactcc gacagctgca
      601 ggtgcccgag cggctgccag cgatggtgcg cgaggtactc gtagcagagg tcctggcgcc
      661 agccgtcgtc gcctccggtc accatccgca tcatccgcgg catgtagctg agcacggtgt
      721 cgccgacgtg cagcgccgcg cggaatcgca ggttctcgac gttccaccgc gtcccgggct
      781 cggcgcggag ccggtcgagg atctcgatcg cctcggcgtc gagccggcgg tcctcgaggc
      841 gggcgtgcgt cccctcgagc atcgcctcgt agagctcctc gtcctggatc acctgggtcc
      901 agacctcgta gacgcgcttc gacgagatcg gcgtcatcgg gtcgcgggcg tgcggtgggc
      961 gtcgaccacc gcgcgggcgc gcttcaggtc gttgttgatc atcggcaccc agctggtgtc
     1021 gacggcgcgc gacacgtggc tgtgggcggg tccctggacc tcgagcacga cggcccggag
     1081 gttgggcgcg cgcggcacga cgtacccgag catctcccac accggctcgg ggaccgagta
     1141 gctgtggaag tcgtgatacc agccatcgga ctgctgacct ccggcgaggt ggatctcgag
     1201 gacgcggtcc agcggcaccg tgcgcaggaa cccccaggcg tcgtagccct ggaagttcct
     1261 cgagttcgcg tacacgttgt ggaggtcgag cagcaggtgg gtctgcgcct tctcgaccat
     1321 ggtcgcgatg aactccgcct cggcgatctc cgtgccggga gccggcgcgt agtagaacgc
     1381 gttctcgtgc aagagcggca tcttcagccg gtcctgcagc gcggccgctc gaccggcgac
     1441 gcgctcgacc tcggcccgcg agaacggcag cgggctcgac cacacgtacg cggtcgcctg
     1501 gaagccgaag aacatgtgat cggcgtacca ggggctgttc atcgcatggg cggccgcgat
     1561 gtggcgcagc acgccggcgg tctcgtcgag cggttcgaac ccgaactcgt tgccgtagtt
     1621 gccatgcgcg atcagcttgt gccgggccgc gatctgctcg agcatcgggc gcatgtacgg
     1681 atcgagggtg tagccggcgt cgagcggccc ggtgaactga tcgcacagca actcggcgta
     1741 ctcgtgcagg ctgggatcga cgcccagcgg cccgagctcg agggcggttg ggttcaactg
     1801 caggccgata ccaacgtgcc cgagcatgcg ctaggagatt cgatgatccg tgaggcgtag
     1861 tcaagctcgg gtccgccacg cggtcacccg ggcagccacg gctccgggtc gaccgcgacc
     1921 aggcgcagct cgctggtgta tcgccggtgc tcggcatcgc agagccacag ctcatcgagg
     1981 tccggcagca tctccgagat cgacacggcc tgtgccttgc gtaccatccg ggccgcgagc
     2041 tccacgaacg tcgggctcgc gaggtcgagg aacagcggct tggtctcctc ggggaccttc
     2101 acgaacagcc gccgcggcag gcccgccgag cgcgcccacg cgcgggcctg gatgaagcgc
     2161 tcgg
//
LOCUS       DASVOR010001803         1679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_223361,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001803 DASVOR010000000
VERSION     DASVOR010001803.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1679
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223361"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="VF516_33550"
     CDS             complement(<1..636)
                     /locus_tag="VF516_33550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827080.1"
                     /note="part of NADH-ubiquinone oxidoreductase complex I;
                     shuttles electrons from NADH, via FMN and iron-sulfur
                     (Fe-S) centers, to quinones in the respiratory chain; NuoF
                     is part of the soluble NADH dehydrogenase fragment, which
                     represents the electron input part of NADH dehydrogenase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit F"
                     /protein_id="HEX8112721.1"
                     /translation="MPAEIGTRIVTARFGNPDARTLPGYERAGGYAQLRKALAMRPED
                     ITAEVKASNLRGRGGAGFATGVKWGFVPKDARAVHLVCNADESEPGTCKDRELIFWDP
                     HLLIEGMVISAHALRAVHNYIYIRGEMMREYAVLQQAVAEAYARGYLGKDILGTGVEV
                     QLTVHRGAGAYICGEETALLNSLEGLRGQPRLKPPFPAVKGLFGNPTIVNNV"
     gene            complement(637..1134)
                     /gene="nuoE"
                     /locus_tag="VF516_33555"
     CDS             complement(637..1134)
                     /gene="nuoE"
                     /locus_tag="VF516_33555"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEX8112722.1"
                     /translation="MSLEFSADAQKKITAACERYPTKQPVVMAALHLAQKEHGHLSDD
                     VLRLVARTLDLPYAHVYGVATFYTMYRRQPGGRQTIRMCTNISCMLRGGYEVLEAFEA
                     KLGLRRGESNAEFTLVEEECIAACANAPCAVVGTEYLLDIKPEQVGELIQRLKAHPHP
                     ESEVV"
     gene            1317..>1679
                     /locus_tag="VF516_33560"
     CDS             1317..>1679
                     /locus_tag="VF516_33560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112723.1"
                     /translation="MPDTPLSRVERIALRFAELANEDSRGKWLQTRFLRGVSYVWVRA
                     AIARRILVEGLDDLMALRPETGVMLVSNHRSFFDQYAMLLACYMGPVPWARHLYFPVR
                     SNFFYDQPLGIAVNALVAG"
BASE COUNT          237 a          582 c          584 g          276 t
ORIGIN      
        1 gacgttgttg acgatcgtcg ggttgccaaa cagccccttg accgccggga acggcggctt
       61 gagccgcggc tggccgcgca ggccctccag cgagttgagc agcgcggtct cctcgccgca
      121 gatgtacgcg ccggcgccgc ggtgcacggt gagctggacc tcgacgccgg tgcccaggat
      181 gtccttgccg aggtacccgc gcgcgtaggc ctcggcgacc gcctgctgca gcacggcgta
      241 ctcgcgcatc atctcgccgc ggatatagat gtagttgtgc accgcgcgca gcgcgtgcgc
      301 cgagatgacc atgccctcga tcagcaggtg cgggtcccag aagatcagct cgcggtcctt
      361 gcaggtgccc ggctcggact cgtcggcgtt gcacaccagg tgcaccgccc tggcgtcctt
      421 gggcacgaag ccccacttga ccccggtggc gaacccggcg ccgccccggc cgcgcaggtt
      481 cgacgccttg acctcggcgg tgatgtcctc ggggcgcatc gcgagcgcct tgcgcagctg
      541 ggcgtagccg cccgcccgct cgtagccggg cagggtcctg gcgtcggggt tgccgaaccg
      601 cgcggtgacg atgcgcgtgc cgatctccgc gggcatctac acgacctccg actcggggtg
      661 gggatgggcc ttcaggcgct ggatcagctc gccgacctgc tcgggcttga tgtcgagcag
      721 gtactcggtg ccgaccaccg cgcacggcgc gttggcgcag gccgcgatgc actcctcctc
      781 gaccagcgtg aactcggcgt tcgattcgcc gcgcctgagg ccgagcttgg cctcgaacgc
      841 ctcgagcacc tcgtagccgc cgcgcagcat gcacgagatg ttggtgcaca tccggatcgt
      901 ctggcggcct cccggctgcc ggcgatacat cgtgtagaac gtcgcgacgc cgtagacgtg
      961 cgcgtacggc aggtcgagcg tccttgcgac caggcggagc acgtcatcgg acaggtggcc
     1021 gtgctccttc tgcgcgaggt ggagcgcggc catcaccacc ggctgcttgg tgggatagcg
     1081 ctcgcaagcc gccgtgatct tcttctgtgc gtccgcggag aattcaagtg acatcgggat
     1141 gttgccttgg cgaaccgcgg gaaaaatagg gggtcgcggg agagacgtca atcggccggc
     1201 agcgcaaacc gcgggcgaat cggaccgcga tcggccggca tcggatggga tccggcccca
     1261 tggcgagccg gtcgcggccg ccgcgggtcg cgccgggacg tgatagaccg tgcccgatgc
     1321 cagatacccc gctcagccgc gtcgagcgga tcgccctgcg gttcgccgag ctcgccaacg
     1381 aggattcccg aggcaagtgg ctgcagactc ggttcttgcg cggcgtgtcg tacgtctggg
     1441 tccgggccgc gatcgcgcgc cgcatcctcg tcgaggggct cgacgacctg atggcgctgc
     1501 gccccgagac cggcgtgatg ctggtgtcca accaccgcag cttcttcgac cagtacgcca
     1561 tgctcctggc ctgctacatg ggcccggtcc cgtgggccag gcacctgtac ttcccggtcc
     1621 gcagcaactt cttctacgac cagccgctcg gcatcgcggt caacgccctg gtcgcgggt
//
LOCUS       DASVOR010001804         1885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_194343,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001804 DASVOR010000000
VERSION     DASVOR010001804.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1885
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194343"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VF516_33565"
     CDS             complement(<1..769)
                     /locus_tag="VF516_33565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112724.1"
                     /translation="MRAALIACGLATLAACHDLPDLGTCGNGIVEQAHGEACDDGGES
                     ATCTASCELKCTASAGIASYVDVGRDRTGKAVFCPDASYRCGIDGVCRAPAGAFEQLA
                     ALVPFDVTTPPVISDVDDDGLVDLVGTSMTRISVRFGSTTGTPLGEVVIQDAPFSDAP
                     YAIFDLPANRPDADQPGTVMAIPTEGVALLRSDGGRFTPELDLPFQIPRSIGEVQGIV
                     VRDPDPAIGDVPIAVQSKSATPSIFLARVAVGLPPLPM"
     gene            complement(769..>1885)
                     /locus_tag="VF516_33570"
     CDS             complement(769..>1885)
                     /locus_tag="VF516_33570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112725.1"
                     /translation="AAADDRAAAEQFFRIGADAYKSGKFDAAAANFDRAYENLKAPEI
                     AFSAAQAHRLQYQADHDPAHLKRAIELYQAYVTGAPDGAKRKDALVYLERLRDALGKI
                     DPRQIAAAQDKPSIYVSIALDHARITIDGAAVERYTSIDVAPGEHVVAASADGYVAQE
                     QRIRVGGKPAMIAFELAPRPATVAIKSQPDARITVDGRPVLLRGTSTEVPAGTRWITV
                     SARGRRPISREVVLVPGQELTLDAPLQPTTQRRAVRWVELGAGALFAGTLATATIALV
                     SDFSAADLRDRKPLTAADAADYQRLRSRRDDLRTASFVLGGAALVTAGVALVLYYADD
                     PSTDALLRPIESKPDRGFTPMTIGGGLGLGIGYAGGF"
BASE COUNT          257 a          676 c          680 g          272 t
ORIGIN      
        1 gcatcgggag cggaggcaac ccgaccgcga cccgcgcgag gaagatgctc ggcgtcgcgc
       61 tcttgctctg caccgcgatg gggacgtctc cgatggccgg atcgggatcc cgcacgacga
      121 tgccctggac ctcgccgatg ctcctgggga tctggaacgg cagatcgagc tcgggcgtga
      181 agcggccgcc gtcggagcgc agcagcgcga ccccctcggt agggatcgcc atcacggtgc
      241 ccggctggtc cgcatcgggg cggttcgcgg gtagatcgaa gatcgcatac ggcgcatccg
      301 agaacggcgc atcctggatc acgacctccc cgagcggcgt cccggtggtg ctgccgaacc
      361 ggacggagat cctggtcatg ctcgtcccga ccagatcgac caggccgtcg tcgtcgacat
      421 cgctgatcac cggcggcgtg gtgacgtcga atggcaccag cgccgcgagc tgctcgaacg
      481 cgcccgcggg cgcgcggcac acgccatcga tgccgcagcg gtagctcgcg tcggggcaga
      541 acacggcctt gccggtccga tcgcggccga catcgacgta cgacgcgatc cccgccgagg
      601 cggtgcactt cagctcgcag ctcgccgtgc acgtggcgct ctcgccgcca tcgtcgcagg
      661 cctcgccgtg agcctgctcg acgatgccgt tgccgcaggt gccgaggtcc gggaggtcat
      721 ggcaggccgc cagcgtcgcg agcccgcatg cgatcagggc agcgcgcatc agaatcctcc
      781 tgcgtagccg atgccaagac cgaggccgcc gccgatcgtc atcggcgtga acccgcggtc
      841 cggcttgctc tcgatcggac gcagcagcgc gtcggtcgat ggatcgtcgg cgtagtacag
      901 gaccagcgcg accccggcgg tgaccagcgc cgcgccgccc agcacgaagc tggccgtgcg
      961 gagatcgtcg cggcggctgc gcagtcgctg gtagtcggcc gcgtccgccg cggtcagcgg
     1021 cttgcggtcg cgcagatccg ccgcggagaa atccgagacc agcgcgatcg tcgccgtggc
     1081 cagcgtccct gcgaacagcg cgccggcgcc gagctcgacc cagcgcaccg cgcgccgttg
     1141 cgtggtcggc tggagcggcg catcgagcgt gagctcctgc cccggcacca gcacgacctc
     1201 gcggctgatc ggccgccggc cgcgcgccga caccgtgatc caccgcgtgc cggccggcac
     1261 ctcggtcgag gtcccgcgca gcagcacggg gcggccgtcg acggtgatcc gcgcatcggg
     1321 ctggctcttg atcgcgaccg tggcgggcct cggcgcgagc tcgaaggcga tcatggcggg
     1381 ctttccgccg acgcggatcc gttgctcctg cgcgacgtag ccgtcggcgc tcgccgccac
     1441 gacgtgctcg ccgggggcga cgtcgatcga cgtgtagcgc tcgaccgccg cgccgtcgat
     1501 ggtgatccgc gcgtggtcga gcgcgatcga gacgtagatc gacggcttgt cctgcgccgc
     1561 cgcgatctgc cgggggtcga tcttgcccag cgcatcgcgc agccgctcga ggtagaccag
     1621 cgcgtccttg cgcttggcgc cgtccggcgc gccggtgacg taggcctggt acagctcgat
     1681 cgcgcgcttg agatgcgccg gatcgtggtc ggcctggtat tgcaggcggt gggcctgcgc
     1741 cgccgagaac gcgatctcgg gcgccttcag gttctcgtag gcgcgatcga agttggccgc
     1801 ggccgcgtcg aacttgccgc tcttgtaggc gtcggcaccg atccggaaga actgctcggc
     1861 cgccgcccgg tcatccgccg ccgcg
//
LOCUS       DASVOR010001805         2231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_544959,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001805 DASVOR010000000
VERSION     DASVOR010001805.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544959"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VF516_33575"
     CDS             <1..356
                     /locus_tag="VF516_33575"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8112726.1"
                     /translation="PWFAVDARPVSDAAFRQVFGDHTQPMAPSGAVVMVSYNDARAYA
                     GTRDGKLLTPDEWDAALRTPGVQPAGDLLEWVESPEAGDEKTKLVRQIGASQTRPDQA
                     HPDVTFRVKKPLPDH"
     gene            377..1321
                     /gene="deoC"
                     /locus_tag="VF516_33580"
     CDS             377..1321
                     /gene="deoC"
                     /locus_tag="VF516_33580"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEX8112727.1"
                     /translation="MTEVDRLVEIITQRVQERLGAQGAQGVAGKLGLPVSLAPRDRGE
                     CNDDSAPGEDCASCGVCVVRRPWSVRAMEGAGAMRVGAAPGVGAVPADLAKLIDHTLL
                     KPEATRDEVVKLCEEARQHRFASVCVNTTWVPLCKALLAGTDVMVCAVVGFPLGAMAP
                     QAKAYEAREAMRQGAREIDMVINIGALRSRDYETVFEDICRVVKAAAPARVKVILETS
                     ALDTEQKIIGCTLAKLAGAAFVKTSTGFGKGGATVEDVALMRRVVGPDVGVKASGGVR
                     TADDAIKMAQAGASRIGASASVAIVTGATGDAAKAKGY"
     gene            1325..>2231
                     /locus_tag="VF516_33585"
     CDS             1325..>2231
                     /locus_tag="VF516_33585"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEX8112728.1"
                     /translation="MLPVQLIHKKRDGGALDDAEIRAFIAGVTDGSLPDYQVAAMLMA
                     IYFRGLDDRELATWADAMTRSGDVIDLSRIARPKVDKHSTGGVGDKISIPLAPAVAAC
                     GVAVPMVSGRGLGHTGGTLDKLSAIPGFRVDLPVERFAAQVDTLGACLIGQTARIAPA
                     DRRLYALRDVTATIESLPMIASSIMSKKLAEGIDGLVIDCKVGRGAFMKTIERARELC
                     ALLRGIGQRAGKRVTCVLTDMNAPIGRTIGNALEIRESIEILRGGGPRDTRELTHALG
                     AEMLVLGGAARDAAEGRAKIERVLGD"
BASE COUNT          320 a          772 c          859 g          280 t
ORIGIN      
        1 cgccgtggtt cgcggtcgac gcgaggccgg tcagcgacgc ggcgtttcgc caggtgttcg
       61 gggaccacac ccagcccatg gccccgagcg gcgcggtcgt gatggtgtcg tacaacgatg
      121 cgcgggcgta cgccgggacc cgcgacggca agctgctcac gcccgacgag tgggacgcgg
      181 cgctccgcac ccccggagtc cagccggccg gcgatctgct ggagtgggtc gagtcgccgg
      241 aggccggcga cgagaagacc aagctggtgc gccagatcgg cgcgtcgcag acccgccccg
      301 accaggccca tcccgacgtc acgttccggg tgaagaagcc gctccccgac cactgaccgg
      361 ctatagtggc gccgccatga ccgaggtcga tcgactggtc gagatcatca cgcagcgggt
      421 ccaggagcgg ctcggcgcgc agggcgcgca gggcgtggcc ggcaagctcg ggctccccgt
      481 gtccctggcg ccccgcgacc gcggcgagtg caacgacgac tccgcgcccg gcgaggactg
      541 cgcgagctgc ggggtgtgcg tggtgcgccg gccgtggtcg gtgcgcgcga tggagggcgc
      601 cggcgcgatg cgggtcggcg ccgcgcccgg agtcggcgcg gtcccggccg atctggccaa
      661 gctgatcgat cacacgctgc tcaagccgga ggcgacgcgc gacgaggtcg tcaagctgtg
      721 cgaggaggcc cgccagcacc gcttcgcgtc ggtgtgcgtc aacacgacgt gggtgccgct
      781 gtgcaaggcg ctgctcgccg ggaccgacgt gatggtgtgc gcggtggtcg ggttcccgct
      841 cggcgcgatg gcgccgcagg ccaaggccta cgaggcgcgc gaggcgatgc gccagggcgc
      901 gcgcgagatc gacatggtga tcaacatcgg cgcgctccgg tcgcgcgact acgagaccgt
      961 gttcgaggac atctgccggg tggtcaaggc ggcggcgccg gcccgggtca aggtgatcct
     1021 cgagaccagc gcgctcgaca ccgagcagaa gatcatcggc tgcacgctgg ccaagctcgc
     1081 cggcgccgcg ttcgtcaaga cctcgaccgg gttcggcaag ggcggcgcga cggtcgagga
     1141 cgtcgcgctg atgcggcggg tggtcggccc cgacgtcggg gtcaaggcgt cgggcggtgt
     1201 ccggacggcg gacgacgcga tcaagatggc gcaggccggc gccagccgga tcggcgcgtc
     1261 ggcctcggtc gcgatcgtga ccggcgcgac cggcgacgcg gccaaggcca agggctacta
     1321 gtcagtgctg cccgtccagc tgatccacaa gaagcgcgac ggcggcgcgc tcgacgacgc
     1381 cgagatccgc gcattcatcg ccggcgtcac cgacggcagc ctccccgact accaggtcgc
     1441 ggcgatgctg atggcgatct acttccgcgg gctcgacgac cgcgagctcg cgacctgggc
     1501 cgacgcgatg acgcgctcgg gcgacgtgat cgacctgtcg cggatcgcgc ggcccaaggt
     1561 cgacaagcac tcgaccggcg gggtcggcga caagatctcg atcccgctgg cgccggcggt
     1621 cgccgcgtgc ggcgtcgcgg tgccgatggt gtcggggcgc ggcctcggcc acaccggcgg
     1681 cacgctcgac aagctgtccg cgatcccggg gttccgggtc gacctgccgg tcgagcggtt
     1741 cgccgcgcag gtcgacacgc tcggcgcctg cctgatcgga cagaccgcgc ggatcgcccc
     1801 ggccgaccgc cggctgtacg cgctgcgcga cgtgaccgcg acgatcgagt cgctgccgat
     1861 gatcgcgtcg tcgatcatgt cgaagaagct cgccgagggc atcgacggcc tggtgatcga
     1921 ctgcaaggtc ggccgcggcg cgttcatgaa gacgatcgag cgcgcccgcg agctgtgcgc
     1981 gctgctgcgc gggatcggcc agcgcgccgg caagcgcgtg acctgcgtgc tgaccgacat
     2041 gaacgcgccg atcggccgga cgatcggcaa cgcgctggag atccgcgagt cgatcgagat
     2101 cctgcgcggc ggcggcccgc gcgacacccg cgagctgacc cacgcgctgg gcgccgagat
     2161 gctcgtgctc ggcggcgccg cgcgcgatgc ggccgagggc cgcgcgaaga tcgagcgcgt
     2221 gctgggcgac g
//
LOCUS       DASVOR010001806         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_280842,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001806 DASVOR010000000
VERSION     DASVOR010001806.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280842"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..415
                     /locus_tag="VF516_33590"
     CDS             143..415
                     /locus_tag="VF516_33590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112729.1"
                     /translation="MRCGSDATLDRYLAGAGGPLIEILDGAHHTIYRDESGVTGEVSD
                     SRDVAGSAGLWTLSVDPAGFAGQFKITLSCRAWGIGAERAPAAGSS"
     gene            complement(513..839)
                     /locus_tag="VF516_33595"
     CDS             complement(513..839)
                     /locus_tag="VF516_33595"
                     /inference="COORDINATES: protein motif:HMM:NF041216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CU044_2847 family protein"
                     /protein_id="HEX8112730.1"
                     /translation="MRLKDFPETTVFIQTPSVPEVPRDDGARAPAATNPGDKVVQSLD
                     DAFVHVRNAAEAIQQALRTMVTSAQSVSVEVGFEVGADAGVVLASTNAGAHVRLVLTW
                     ARTHYK"
     gene            979..>1515
                     /locus_tag="VF516_33600"
     CDS             979..>1515
                     /locus_tag="VF516_33600"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8112731.1"
                     /translation="MTEPEIRTSSITIPVADGTTMGGYLAAPAAPGPRPAIFVIQEIF
                     GVNAHIRDVTERFARAGYIAVAPEIFHRFAPGYQGSYDDIQASIAMTAKLTPEGVRAD
                     LAATHAWLAARNDVRAGRVAAIGYCMGGRLTWVANAALPLGAAVSYYGGGIATNHLEL
                     AGALQGPQLLVWAGNDPWI"
BASE COUNT          234 a          527 c          507 g          247 t
ORIGIN      
        1 ccgccggcga tacggagctt cgcgaaagcc tttcgtgact ttcaagaagc ctttgagggc
       61 tttccaggtc ctcgaaaagg cgcgtgcgag cgccgggact cgaacccgga ccctgttacg
      121 gacaggaacc taaatccagt cggtgcgctg cggctccgac gcgacgctcg accgctacct
      181 cgccggcgcc ggcggcccgc tgatcgagat cctcgatggc gcgcaccaca cgatctaccg
      241 cgacgagtcc ggcgtgaccg gcgaggtcag cgactcgcgg gacgtcgcgg ggagcgccgg
      301 cctgtggacg ctgtcggtcg accccgccgg gttcgccggc cagttcaaga tcacgctgtc
      361 gtgccgcgcc tgggggatcg gcgcggagcg cgcgccggcc gccggctcgt catgagtctt
      421 tcacggtctg gtgtgctcgc actggcatcg atcggtatgc acacaagtcg atagtctgcg
      481 attgccgagc tgtgccgctc tgccaaccgg ggtcacttgt aatgagtacg tgcccatgtc
      541 aggacgagtc tcacatgcgc gcctgcattc gtggatgcca gaacgacgcc ggcatcagcg
      601 cccacctcga accccacctc gacggagacc gactgggcgc tcgtcaccat ggtcctgagt
      661 gcttgctgga tcgcctccgc tgcattgcgg acatggacga aggcgtcatc cagcgattgt
      721 acgaccttgt ctcctggatt cgtggcggcg ggtgcccgtg cgccgtcgtc acgtgggact
      781 tcggggaccg aaggcgtctg tatgaacaca gtggtttctg gaaagtcctt gagacgcaat
      841 ggagtcatca tggtcctata gcaggaatct gtctgcagca ctcgacggcg gtctcgcggc
      901 cgcgcggcgc aggtcgaccg ccggcgagag agcgcgatgg gggtgagtcc ggaacgcagc
      961 tgtgatagat ccgcggccat gaccgagccc gagatccgca cgtcctccat caccatcccg
     1021 gtcgccgatg gcaccacgat gggcggctac ctcgccgccc cggcggcgcc ggggccgcgc
     1081 cccgcgatct tcgtcatcca ggagatcttc ggcgtgaacg cccacatccg cgacgtgacc
     1141 gagcgcttcg cgcgcgccgg ttacatcgcg gtcgcgcccg agatcttcca ccgcttcgcc
     1201 cccggctacc aggggtcgta cgacgacatc caggccagca tcgcgatgac cgcgaagctc
     1261 acgccggagg gcgtgcgggc cgacctcgcc gccacccacg cctggctcgc cgcgcgcaac
     1321 gacgtgcgcg ccggccgcgt cgccgcgatc ggctactgca tgggcgggcg gctcacgtgg
     1381 gtcgccaatg ccgcgctgcc gctcggcgcc gcggtgtcgt actacggcgg cggcatcgcg
     1441 accaaccacc tcgagctcgc cggcgcgctg cagggcccgc agctgctggt gtgggccggc
     1501 aacgatccgt ggatc
//
LOCUS       DASVOR010001807         1782 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_311308,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001807 DASVOR010000000
VERSION     DASVOR010001807.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1782
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311308"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VF516_33605"
     CDS             complement(<1..1568)
                     /locus_tag="VF516_33605"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8112732.1"
                     /translation="MSRSPEQLEAYLRRTAAALLETEARLERERAERREPIAIVGVGC
                     RLPGGVSNPDALWSLLAAGRDAIGPLPERWSHLALFDRDPDAPGKCYAREGGFLDEID
                     RFDAAFFGISPREARWMDPQQRIALEVAWHALEDTGIRPSSLHRTRTGVFLGTMGSDY
                     ALAARSELAMLSGYQSTGNASSVLSGRLSYHFGLNGPSLTIDTACSSSLVALHLACES
                     LRRGESDVVLAGGVTVMCEPAALVEFSRLKGLSPGGRCRSFAADADGMAWAEGCGLVV
                     LKRLSSARRDGDRVRAIIRGSAVNQDGRSQGLTAPNGPAQERVIHDALRVSGLEVDDI
                     DAIEAHGTGTSLGDPIEAGALVNVFATRSVVEPVHLGSIKSNLGHTQAAAGVVGLIKM
                     VLALEHEQLPATLHATSPTPLVDWAASKLQLVQTARPWPRRDRDRRAGISAFGISGTN
                     AHVVIEEAPAPEAPGAVTAGGSIQAPVAVPVVLSATDPRALAIQAAQLDRWLDIAEPR
                     LVDLAFTRAACRDAL"
     gene            complement(1565..>1782)
                     /locus_tag="VF516_33610"
     CDS             complement(1565..>1782)
                     /locus_tag="VF516_33610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112733.1"
                     /translation="LERAGVVAELLGRIAPAHLEDPGVAAAVVGLKHHLERVIGTAAT
                     AGRAAERVTGATDDLFALLDAKLGGTP"
BASE COUNT          236 a          648 c          666 g          232 t
ORIGIN      
        1 agcgcgtcgc ggcaggccgc gcgcgtgaac gcgaggtcga cgaggcgtgg ctcggcgata
       61 tctagccagc ggtcgagctg cgcggcctgg atcgcgagcg cgcgcggatc ggtcgcggac
      121 agcacgaccg gcacggcgac cggcgcctgg atcgatccac cggcggtcac cgcgccgggg
      181 gcctcgggcg ccggcgcctc ctcgatcacg acgtgcgcgt tggtcccgct gatcccgaac
      241 gcggagatcc cggcgcggcg atcgcggtcc cggcggggcc atggccgggc ggtctgcacc
      301 agctggagct tgctggccgc ccagtcgacg agcggggtcg gggacgtcgc gtgcagggtc
      361 gccgggagct gctcgtgctc gagcgcgagc accatcttga tcaggccgac cacgccggca
      421 gcggcctggg tatggccgag gttcgacttg atcgatccga gatggaccgg ctcgaccacg
      481 ctgcgcgtcg cgaacacgtt gaccagcgcg ccggcctcga tcgggtcgcc gaggctcgtg
      541 ccggttccat gggcctcgat cgcgtcgatg tcgtcgacct cgagcccgct cacgcgcagc
      601 gcgtcgtgga tcacgcgctc ctgcgccggc ccgttcggcg cggtcaggcc ctggctgcga
      661 ccgtcctgat tgaccgcgct gccgcggatg atcgcgcgga cccggtcgcc gtcgcggcgt
      721 gccgaggaca gccgcttgag gacgacgagg ccgcaaccct cggcccaggc catgccgtcc
      781 gcatcggccg cgaagctccg gcaccgcccg ccgggcgaca ggcccttgag cctgctgaac
      841 tcgacgagtg ccgcgggctc gcacatcacg gtcacgccgc ccgcgaggac gacgtcggac
      901 tcaccgcggc gcagcgactc gcacgcgagg tgcagcgcga cgagcgacga cgagcacgca
      961 gtgtcgatcg tcagggacgg gccgtttagc ccgaagtggt agctcagccg ccccgacagc
     1021 acgctgctcg cgttgccggt gctctggtag ccggacagca tggcgagctc gctgcgcgcc
     1081 gcgagggcgt agtccgagcc catcgtgcca aggaacacgc cggtgcgcgt ccggtggagc
     1141 gagctcgggc ggatgccggt gtcctcgagt gcgtgccacg cgacctcgag tgcgatccgc
     1201 tgctgcggat ccatccacct ggcttcgcgc ggcgagatgc cgaagaacgc ggcgtcgaac
     1261 cgatcgatct cgtcgagaaa cccgccctcg cgtgcgtagc acttgcccgg agcgtcggga
     1321 tcgcgatcga acagcgcgag gtgtgaccag cgctccggca gcgggccgat cgcatcgcgg
     1381 ccggccgcga ggaggctcca cagcgcgtcc gggttgctca cgccgccggg caggcggcac
     1441 ccgaccccga cgatcgcgat cggctcgcgg cgctcggcgc gctcgcgctc gagccgcgcc
     1501 tcggtctcga gcagggccgc ggcggtgcgc cgcaggtatg cctcgagctg ctcgggcgac
     1561 cggctcacgg cgtgcctccg agcttggcgt cgagcagggc gaatagatcg tcggtcgccc
     1621 cggtcacccg ctcggcggcg cgaccggcgg tcgcggccgt gccgatcacg cgctcgaggt
     1681 ggtgcttgag gccgaccacc gccgcggcga cgccgggatc ctcgaggtgc gccggcgcga
     1741 tccggcccag cagctcggcg acgacgccgg cccgctcgag cg
//
LOCUS       DASVOR010001808         3599 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_223422,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001808 DASVOR010000000
VERSION     DASVOR010001808.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3599
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223422"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..1542
                     /locus_tag="VF516_33615"
     CDS             67..1542
                     /locus_tag="VF516_33615"
                     /inference="COORDINATES: protein motif:HMM:NF021597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2330 domain-containing protein"
                     /protein_id="HEX8112734.1"
                     /translation="MRRAVITAAVALASAPGSASAFCGFYVTGSDQQLLADATQVVLM
                     RKGTRTVLSMQNNYKGPPEAFAMVIPVPVVLHEPDVKTLPPEVFASVERMGAPRLVEY
                     WEQDPCQPERLYAVARAGGAPPPAAMPRAARPADLGVTIEAQFTVGEYQIVILSAKES
                     TGLDTWLRREKYNIPRGAEPLLRPYVESGSKFFVAKVDPRKVRFVDGRANLSPLRFHY
                     DSEQFVLPIRLGLANSGGTQDLIVSILAPGQRYELANYRNVTIPTNLDVKDSVRDHFG
                     AFYAALFDRTLERNPGAVVTEYAWQATTCDPCPGPTLNANDFMTLGADVLEPSQQIAA
                     PPAPAGGRPRPMPPNVRRMPMGWGMDFVLTRLHARYGRDLKDDLVFRAAPPIVGGREF
                     VRDGGRLEEGAQPSGVNNFQARYAIRHRWTGPIACEKPRRGIWGGPPNGGGTSPQPAL
                     NLAFAPRGAVQLASTIHGDVPEIGLRKGVLEPVPAPAQKPK"
     gene            1602..1901
                     /locus_tag="VF516_33620"
     CDS             1602..1901
                     /locus_tag="VF516_33620"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX8112735.1"
                     /translation="MRIKSALLLCSLFAVACAGSPPPPPAAPSPAPTAASSAPLVAPG
                     EAKVGDRTKCPVSGHEFVVKADSPHAEYNGKTYYFCCPDCPKAFEANPEKFATKA"
     gene            1927..2952
                     /locus_tag="VF516_33625"
     CDS             1927..2952
                     /locus_tag="VF516_33625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112736.1"
                     /translation="MSIDFPRPRIAALQDHAARLYESRRSGTSLASLARDLLLAFFDV
                     CAHAGLDRVLADLAQVFPPLDLSDRSALATHEAVLPALLAQLETIDLDGGGPRGTKPR
                     QLVDCVVAALGLTLVDEPDRTIMLDDAVRVDVTRALAAVVDVELAAPKLRADIIADAR
                     ARCDLRYHAAFDRLAAQLDERGLQLVKQPKVPIDALHATQYALFDARNAVIARVAGAA
                     LDRARDVLARADADAGARIDRPITLRMTPREVAILRACDARVSKTPARVLQSLLDSLT
                     DLLPIAWRAPAQPALPYAASRTFAVGDVIDHPKFGRGKVIATVLKRIDVEFADGTHTL
                     AHAPPSK"
BASE COUNT          513 a         1357 c         1206 g          523 t
ORIGIN      
        1 cggcatccta cgccattgat cgccggcgcg gaacgcgctc tgctcggcgg ccggaggagg
       61 cacgccatga ggagagctgt catcaccgcc gctgtcgcgc tcgcgagtgc gccgggcagc
      121 gcgagtgcgt tctgtggctt ctacgtcacc ggcagcgatc agcagctgct cgccgacgcc
      181 acgcaggtcg tgctgatgcg caagggcacg cgcacggtgc tgtcgatgca gaacaactac
      241 aagggaccgc ccgaggcgtt cgcgatggtg atcccggtgc cggtcgtgct ccacgagccg
      301 gacgtcaaga cgctgccgcc cgaggtgttc gccagcgtcg agcgcatggg cgcgccgcgc
      361 ctggtcgagt actgggagca ggacccgtgc cagcccgagc ggctgtatgc ggtggcgcgg
      421 gcgggcgggg cgccgccgcc ggcggcgatg ccgagggctg ccaggcccgc cgacctcggc
      481 gtcaccatcg aggcccagtt cacggtcggc gagtaccaga tcgtgatcct gtcggccaag
      541 gagtcgaccg ggctcgacac ctggctgcgc cgcgagaagt acaacatccc gcgcggcgcc
      601 gagccgctgc tgcggcccta cgtcgagagc ggctcgaagt tcttcgtcgc caaggtcgac
      661 ccccgcaagg tcaggttcgt cgacggccgc gccaacctgt cgccgctgcg gttccactac
      721 gacagcgagc agttcgtcct gccgatccgg ctcgggctcg ccaacagcgg cggcacccag
      781 gacctgatcg tgagcatcct cgcgcccggc cagcgctacg agctcgccaa ctaccgcaac
      841 gtcacgatcc cgaccaacct cgacgtcaag gactccgtcc gcgatcactt cggcgcgttc
      901 tatgccgcgc tgttcgaccg cacgctggag cgcaaccccg gcgcggtggt caccgagtac
      961 gcctggcagg ccacgacctg cgatccgtgc cccggcccga cgctcaacgc caacgacttc
     1021 atgaccctcg gcgccgatgt gctcgagccg tcgcagcaga tcgccgcgcc gcccgcgccc
     1081 gccggcggcc gcccgcggcc gatgccgccg aacgtgcgcc gcatgccgat gggctggggc
     1141 atggacttcg tgctcacccg gctccacgcg cgctacggcc gcgacctcaa ggacgacctc
     1201 gtgttccgcg cggcgccgcc gatcgtcggc ggccgcgagt tcgtgcgcga cggcggccgg
     1261 ctcgaggagg gcgcgcagcc gtcgggcgtg aacaacttcc aggcgcgcta cgcgatccgc
     1321 caccgctgga ccggcccgat cgcctgcgag aagccgcgcc ggggcatctg gggcggcccg
     1381 cccaatggcg gcggcacgtc gccccagccc gcgctcaacc tggcgttcgc gccgcgcggc
     1441 gcggtccagc tcgcgtccac gatccacggc gacgtccccg agatcggcct gcgcaagggc
     1501 gtcctcgagc cggtcccggc gcccgcgcag aagccgaaat agcgccgcgc tgatcggtga
     1561 gatcgtaacg gacgtccccg gccgcaggta cactccggcg catgcgaatc aagagcgcgc
     1621 tcctcctctg ctccctcttc gccgtcgcgt gcgcaggctc cccgccgccg ccgccggccg
     1681 ctcccagccc ggcccccacc gccgcgtcgt cggcccccct ggtcgcgccg ggcgaagcca
     1741 aggtcgggga tcgcaccaag tgccctgtct ccggacacga gttcgtcgtc aaggcagact
     1801 cgccgcacgc cgagtacaac gggaagacgt attacttctg ctgccctgac tgcccgaagg
     1861 cattcgaggc aaaccccgag aagttcgcca ccaaggcctg atggcccggg tggggcgcct
     1921 gcttccatgt cgatcgactt tccgcgaccc aggattgccg cgctccagga ccacgccgcg
     1981 cgcctgtacg aaagccgcag gtcgggcacg agcctcgcct cgcttgcacg cgatctgctg
     2041 ctggcgttct tcgatgtctg cgcccacgcc gggctcgatc gcgtgctggc cgacctcgcg
     2101 caggtgttcc cgccgctcga cctcagcgat cgctcggcgc tcgcgaccca cgaggcggtg
     2161 ctgccggccc tgctcgcgca gctcgagacg atcgatctcg acgggggtgg cccgcgtggc
     2221 acgaagccgc gccagctggt ggattgcgtc gtcgccgcgc tcggcctgac gctggtcgac
     2281 gagccggacc ggacgatcat gctcgacgac gccgtgcgcg tcgacgtcac ccgcgcgctg
     2341 gccgccgtgg tcgacgtcga gctcgcggcg ccgaagctgc gcgcggacat catcgccgac
     2401 gcccgggcgc gctgcgacct ccgctaccac gcggcgttcg atcgcctcgc cgcccagctc
     2461 gacgagcgcg ggttgcagct cgtcaagcaa cccaaggtac cgatcgacgc cttgcacgcc
     2521 acgcagtacg cgctgttcga tgcccgcaac gccgtcatcg cgcgggtcgc cggcgctgcg
     2581 ctcgatcgcg cgcgcgacgt gctcgcccgc gccgacgccg acgccggcgc ccggatcgat
     2641 cgaccgatca cgcttcgcat gacgccgcgc gaggtcgcga tcctgcgcgc ctgcgatgcc
     2701 cgggtcagca agacccccgc gcgggtcctg cagtccctgc tggatagcct caccgatctc
     2761 ctgccgatcg cctggcgcgc gcctgcgcag ccggcgctgc cctacgccgc gagccggacg
     2821 ttcgccgtgg gcgacgtcat cgaccatccg aagttcggac gcggcaaggt aatcgcgacc
     2881 gtcttgaagc gaatcgacgt cgagttcgcc gatggcacac atacgctcgc tcacgccccg
     2941 ccatcgaagt agcggctcga ccgaggtccg gcaccaggcg cgacccggca gggacgcggc
     3001 gccgggcaaa cccgcgtttc tcgatcatcg ggggcgctgt tggcggtgtc gcccgagcac
     3061 accgccccac gtcctcctgg ctcaacgcgc caggttctcg cgtcacgcgc gcgcaatcgg
     3121 ctgatacagc ctgaataact gcctcacgag ctcatcgagc tcggccggga ggacttcgat
     3181 cggatcgact tgactcgtcg tgtgccagga ggcgatggag gcagcgcccg gcgcggccgt
     3241 cgccggcagc ccttgcggcg gtcagaactg catgccgagg cccacgccca ggtgcgggta
     3301 cgacagctcc gtgtcgtcgc gaggcggtcc tctgcgttct tctggtcgac gtgcaggagc
     3361 gagcgctgat agccgatcct attgtctgca ggagccaccg gtccgcatgc ggcgctcggc
     3421 gcgcgctacc tcgacgcgat gcgcaacgcg gcgaacttcg cgttcggcaa ccggctgttt
     3481 ctcggcctca tggccgtgcg cgtgctgtcg gaggtgctcg gtcgcgatgt cggcgcgcgc
     3541 ctggtctacg acgcgccgca caacctgatc tgggattacc ggcgcggcga tattcacct
//
LOCUS       DASVOR010001809         4767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_194410,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001809 DASVOR010000000
VERSION     DASVOR010001809.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4767
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194410"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="VF516_33630"
     CDS             complement(<1..818)
                     /locus_tag="VF516_33630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112737.1"
                     /translation="MIPEPLGAPDDGRDRPRLRLKLRTKLAVAMLFAALVPAVVVALL
                     ATGVILSSLETGLREDADRQLIVGLNLILRSIERLGDETVQLAESGELAAALSSSAAL
                     DAWIAHESAHVPSARLQLIEPSGEIRFDHVFGGAESRFRDTGVVPGDPAIETGRKWGR
                     GISLVTRGERVVVRAVSPIVDAGLALRGVLVLSMPLDADFADGIKGALSADVLLGGPS
                     GRMQTTFRVGLGGRTETFDLPDADRAAALAGQRVIRNLDVADGQYLLAATALHDR"
     gene            complement(815..2401)
                     /locus_tag="VF516_33635"
     CDS             complement(815..2401)
                     /locus_tag="VF516_33635"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8112738.1"
                     /translation="MPRALLIALGAAALACDAAPGTLHHRRDPAALVVAQAVDVTGLD
                     LARVIDNESIEVGEIIFEGLARWLPGTLDVEPGLASAWQVSADGLRWTFQLRSGVVFH
                     DGTPLDADAVVFSFERLLDPRHPNYLPGDDAVYWRGLLRDVRRVTALDPATVVIEIDR
                     PYAPLLGELVMFPIVSPTAVRRWGNDFTRHPVGTGAFAFETWSPSEQVVLHRFDRYWG
                     PRPALDRIVFRVVVDPRQRLIDLQSDSVDLAASIQLDEQSFVELHPDLRLHHAPASQI
                     TFLAFNLDHPPFDDSRVRRAISHAINREPIVKLAFQGRAVAADSALPPGQWGHHVPAT
                     HYDYEPALARRLLDEAIAAHAFDPARVYKLYAPITPRAYLFVPERVARYFQVALAQIG
                     VRVELVLQPIQQQIASLARGEHDLALSGWSGDTGDPDNFLYVLLHSDNAVPGSAQNIA
                     FYRNPEVDRLLVAAQAAVEQPVRKELYGEVQDRVARDAPWVPIAYPELVVAGRAEIEQ
                     VILTPLGHPLYTMIRRKEPR"
     gene            2604..3527
                     /locus_tag="VF516_33640"
     CDS             2604..3527
                     /locus_tag="VF516_33640"
                     /inference="COORDINATES: protein motif:HMM:NF028121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter TatT component family protein"
                     /protein_id="HEX8112739.1"
                     /translation="MHARLAFAAILVVSLSACDIGRITVGTTAHILVRAQPALNQESD
                     YQLAHDAIPGTLKTIEGFWVVDPDNAELLGLLTEGYCQYGTAFVEDDWEVARFAKKLD
                     EIEYHNARATNIFTRCLNYALRQLGERWQTELFGAPDGVARLAHDTDAGKRDALMWAA
                     LALGSIINHNLSRVELLAYQPTVKLMLDRVLEIDRSHPPSRPDYAALPHIAFAMLYSA
                     TGVQTGGKPAEAKAEFEKALELTGNRFLLARALMGYRLGLVNNDQKFFHDQLKQVLET
                     PPSVWPEQRLANEVAHRRARRYLSHEKELFQ"
     gene            3524..4573
                     /gene="dctP"
                     /locus_tag="VF516_33645"
     CDS             3524..4573
                     /gene="dctP"
                     /locus_tag="VF516_33645"
                     /inference="COORDINATES: protein motif:HMM:NF015445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein DctP"
                     /protein_id="HEX8112740.1"
                     /translation="MTGQDSGHSSPSIQLLGKLLGLCAAVTLTAARPARADNIELRIA
                     TLAPSGSPWMEVLDKAANETKEKTGGRVTLKYFEGGQQGDERDFVRKINLGQLDGAAV
                     TAVGLSMVDESIRVLELPMMFESAEEFDYVADKLWPYFQKKFEAKGFLLADRGEVGWI
                     YFLSKNKVEKLADLRGQKLWLWGDDRLVGAVFKKLGLNGVPLGVPEVDAALTSGKIDA
                     CYGSPVAAVALQWYSKVKYMTSMPMSFAMGANLISLKAYKKLSPEDAKLVEGISRANA
                     KKLRKVIRKDNEDAKGTMSRKGVTVVQTPIAMVDEFTKASTEIWNELAGKLYSKEELK
                     MVLDARDEYRAKHKK"
BASE COUNT          776 a         1665 c         1693 g          633 t
ORIGIN      
        1 cggtcgtgca gcgcggtcgc ggcgagcagg tactgtccgt cggcgacgtc gaggttgcgg
       61 atcacgcgct ggccggcgag cgccgccgcg cggtcggcgt cggggaggtc gaacgtctcg
      121 gtccgcccgc cgagcccgac ccggaacgtg gtctgcatcc ggccggacgg cccgccgagc
      181 aggacgtcgg ccgacagcgc gcccttgatg ccgtccgcga agtcggcgtc gagcggcatc
      241 gacagcacga gcacgccgcg cagcgccagc ccggcgtcga cgatcggcga caccgcgcgc
      301 acgaccacgc gctcgccgcg cgtcaccagc gagatcccgc ggccccactt gcggccggtc
      361 tcgatcgccg gatcgcccgg cacgaccccg gtgtcgcgaa accgagactc ggcgccgccg
      421 aacacgtggt cgaaccggat ctcgcccgac ggctcgatca gctggagccg cgccgacggc
      481 acgtgcgccg actcgtgggc gatccaggcg tcgagcgcgg cggagctcga cagcgccgcg
      541 gcgagctcgc ccgactccgc gagctggacg gtctcgtcgc ccagccgctc gatgctgcgc
      601 aggatcaggt tgaggccgac gatcagctgg cggtcggcgt cctcgcgcag gccggtctcc
      661 aggctcgaca ggatcacccc ggtcgcgagc agcgcgacga ccaccgccgg gaccagcgcc
      721 gcgaacagca tcgcgaccgc gagcttggtc cgcagcttga gccgcagccg gggccggtcc
      781 cggccgtcgt cgggcgcgcc gagcggctcc gggatcaccg cggctccttg cggcggatca
      841 tcgtgtacag cgggtggccc agcggggtca ggatcacctg ctcgatctcc gcgcgccccg
      901 cgaccaccag ctcggggtac gcgatcggca cccacggtgc gtcgcgggcg acccggtcct
      961 ggacctcgcc gtacagctcc ttgcgcaccg gctgctcgac cgcggcctgg gccgcgacca
     1021 gcaggcggtc gacctccggg ttgcggtaga acgcgatgtt ctgggccgag ccgggcaccg
     1081 cgttgtcgct gtgcagcagc acgtagagga agttgtcggg atcgccggtg tcgccgctcc
     1141 accccgacag cgcgaggtcg tgctcgccgc gcgcgagcga cgcgatctgc tgctggatcg
     1201 gctggaggac gagctcgacg cgcacgccga tctgggccag cgcgacctgg aagtagcgcg
     1261 cgacccgctc gggcacgaac aggtacgcgc gcggcgtgat cggcgcgtac agcttgtaga
     1321 cccgcgccgg gtcgaacgcg tgcgccgcga tcgcctcgtc gagcagccgc cgggcgagcg
     1381 cgggctcgta gtcgtagtgg gtcgccggca cgtggtggcc ccactggccg ggcggcagcg
     1441 ccgagtcggc ggcgaccgcg cggccctgga aggcgagctt gacgatcggc tcccggttga
     1501 tcgcgtggct gatcgcgcgg cggacccggg agtcgtcgaa cggcggatga tcgaggttga
     1561 acgccaggaa ggtgatctgg ctggccgggg cgtggtggag ccgcaggtcg gggtggagct
     1621 cgacgaacga ctgctcgtcg agctggatcg acgcggcgag gtcgaccgag tccgactgca
     1681 ggtcgatcag ccgctggcgc ggatcgacca cgacccggaa cacgatgcgg tcgagcgccg
     1741 ggcgcgggcc ccagtagcgg tcgaaccggt ggagcacgac ctgctcgctg gggctccagg
     1801 tctcgaacgc gaacgcgccg gtgccgaccg ggtggcgggt gaagtcgttg ccccagcgcc
     1861 ggaccgcggt cggcgagacg atcgggaaca tcacgagctc gccgagcagc ggggcgtacg
     1921 gccggtcgat ctcgatcacg accgtcgccg gatcgagcgc ggtgacccgc cggacgtcgc
     1981 gcagcaggcc ccgccagtac accgcgtcat cgccggggag gtagttcgga tgcctggggt
     2041 cgagcaggcg ctcgaacgag aacaccacgg cgtcggcgtc gagcggggtg ccgtcgtgga
     2101 acaccacgcc ggagcggagc tggaacgtcc agcgcaggcc gtcggccgag acctgccagg
     2161 ccgaggccag cccgggctcg acgtcgagcg tgccgggcag ccaccgcgcc aggccctcga
     2221 agatgatctc gccgacctcg atcgattcgt tgtcgatcac ccgggccagg tcgagcccgg
     2281 tgacgtcgac cgcctgggcg accacgagcg ccgccgggtc acggcggtgg tgcagggtcc
     2341 cgggggcagc gtcgcaggcc agtgcggcgg cacccagcgc gatcaggagc gcgcgcggca
     2401 tcgcacgcga ctgtaccatc gcccggccgc ccagccggag attgactctc gccggtcact
     2461 gccttacggt cgcgcgagcg tgtgcggtcc atgaatgttg ggcccgcgcc accgtcacag
     2521 ctcatgtcac aactcgggtc aacattcctg gaccagaatg gtcagggcca gaacggcctg
     2581 ggccagaagg gatgcgttcg tcgatgcacg cacggttagc tttcgccgcg atcctcgtcg
     2641 tcagcctgtc ggcctgcgac atcggccgga tcaccgtcgg gacgacggcg cacatcctgg
     2701 tgcgcgccca gcccgcactc aaccaggagt cggactacca gctcgcgcac gacgcgatcc
     2761 ccggcacgct caagacgatc gaggggttct gggtggtcga cccggacaac gccgagctgc
     2821 tcggcctctt gaccgagggc tactgccagt acgggaccgc gttcgtcgag gacgactggg
     2881 aggtcgccag gttcgccaag aagctcgacg agatcgagta ccacaacgcg cgcgcgacca
     2941 acatcttcac ccgctgcctc aactacgcgc tgcgccagct cggcgagcgc tggcagaccg
     3001 agctgttcgg cgctccggac ggggtcgccc ggctcgccca cgacaccgac gccggcaagc
     3061 gcgacgcgct gatgtgggcg gcgctggcgc tcggttcgat catcaaccac aacctcagcc
     3121 gggtcgagct gctggcctat cagccgaccg tgaagctgat gctcgaccgc gtcctggaga
     3181 tcgaccgctc tcacccgccg agccgtcccg attacgccgc gctgcctcat attgcgttcg
     3241 ccatgctgta cagcgcgacc ggcgtccaga ccggcggcaa gcccgccgag gccaaggccg
     3301 agttcgagaa ggcgctcgag ctgaccggga accgcttcct gctcgcccgc gccctgatgg
     3361 gctaccggct cggcctggtc aacaacgacc agaagttctt ccacgaccag ctcaagcagg
     3421 tgctcgagac cccgccgtcg gtgtggcccg agcagcgtct cgccaacgag gtcgcccatc
     3481 gccgggcgcg ccggtatctc tcgcacgaaa aggagctatt ccaatgacgg ggcaagattc
     3541 gggacattca tcaccgtcga tccagctgct cggcaagctg ctcggcctgt gcgccgccgt
     3601 cacgctgacc gccgcgcgcc cggcgcgcgc cgacaacatc gagctccgca tcgcgacgct
     3661 ggcgccgtcc ggaagcccgt ggatggaggt gctcgacaag gcggcgaacg agaccaagga
     3721 gaagaccggc ggccgggtca cgctcaagta cttcgagggc ggccagcagg gcgacgagcg
     3781 cgacttcgtc cgcaagatca acctcggcca gctcgacggc gcggcggtca ccgcggtcgg
     3841 cctgtcgatg gtcgacgagt cgatccgcgt gctcgagctg ccgatgatgt tcgagtcggc
     3901 cgaggagttc gactacgtcg ccgacaagct gtggccgtac ttccagaaga agttcgaggc
     3961 caagggcttc ttgctcgccg accgcgggga ggtcggctgg atctacttcc tgtccaagaa
     4021 caaggtcgag aagctcgccg acctgcgcgg ccagaagctg tggctgtggg gcgacgaccg
     4081 cctggtcggc gcggtgttca agaagctcgg gctcaacggc gtgccgctcg gcgtccccga
     4141 ggtcgacgcc gcgctgacct cgggcaagat cgacgcgtgc tacggctcgc cggtcgccgc
     4201 ggtcgccctg cagtggtact cgaaggtcaa gtacatgacg tcgatgccga tgagcttcgc
     4261 gatgggcgcc aatctgatct cgctcaaggc ctacaagaag ctgtcgcccg aggacgccaa
     4321 gctggtcgag ggcatcagcc gggccaacgc caagaagctg cgcaaggtga tccgcaagga
     4381 caacgaggac gccaagggca cgatgagccg caagggcgtc accgtggtcc agaccccgat
     4441 cgcgatggtc gacgagttca ccaaggcgtc caccgagatc tggaacgagc tcgccggcaa
     4501 gctgtactcg aaggaagagc tcaagatggt gctcgacgct cgcgacgagt atcgcgccaa
     4561 gcacaagaaa tagtatttac cgttgaaagc ccggcgaact gcccactacc atggaccggg
     4621 tgagcgactc aggcgacggc gcgcgacagg gaccgcccca tccaccgtct gatccagcgg
     4681 aggccccggg cgccggcccg cccgatcccg gccgcgcgcc ggagcacacc gcgccggccg
     4741 aggccgaggc gccggccgag gccgggg
//
LOCUS       DASVOR010001810         1634 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_311393,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001810 DASVOR010000000
VERSION     DASVOR010001810.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1634
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311393"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..419)
                     /locus_tag="VF516_33650"
     CDS             complement(<1..419)
                     /locus_tag="VF516_33650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112741.1"
                     /translation="MVKVLESRQPIEALRKFLARIPGQATMEQGQIALGSAQLMLLPI
                     AHEHRGGDEVKQLLDLVLARWDAFPDRSGFHAQEFLRNAFAAVGDDRGRIARLSALVP
                     PDATSELRFNMAGAYAIAGDRPAMLHALEAALAIGATA"
     gene            412..828
                     /locus_tag="VF516_33655"
     CDS             412..828
                     /locus_tag="VF516_33655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112742.1"
                     /translation="MTTELPPLSRAGLRVGGREGGLRADGLAGATCLAAMVGVAVVFP
                     AADVVGPVFFLLAGVLLTAGACLAALFLAVDFALAGVALAGVALAGVALAGVARAGAA
                     LARSFALARDRGDERARAIATDAIMPRRARTRSGAP"
     gene            889..>1634
                     /locus_tag="VF516_33660"
     CDS             889..>1634
                     /locus_tag="VF516_33660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112743.1"
                     /translation="MRILAVAVILAACGDNRVEGPPPPGCDAWHQWGNNASHDGASCV
                     PGQPLRTALSSTVYDPFLADEVADARGVLLVHYQAPLIDGDDLYMMTKHGTYTPCNVG
                     SDGPSCGDPDELHRLDSQIWAEQRFVISPSGALAQQWSFDSDWKPVPGIGFEPMFQPA
                     LAGGLIAIPGAGGALWELDRLSGQVVRHVQPLGAAIDPDIYVAGGLAAGPDGTLYYSA
                     FRDVQLPATPQAWLVAVAPDGTVRTADYAAL"
BASE COUNT          189 a          615 c          596 g          234 t
ORIGIN      
        1 gcggtggcgc cgatcgcgag cgcggcctcc agcgcgtgca gcatcgccgg ccgatcgccc
       61 gcgatcgcgt acgcgccggc catgttgaac cgcagctcgc tggtcgcgtc gggcgggacc
      121 agcgccgaca gccgggcgat ccgaccgcga tcgtcgccga ccgcggcgaa cgcgttgcgc
      181 aggaactcct gggcgtggaa cccgctgcgg tcggggaacg cgtcccaccg cgcgagcacc
      241 aggtcgagca gctgcttgac ctcgtcgccg ccgcggtgct cgtgggcgat cgggagcagc
      301 atcagctgcg ccgagccgag cgcgatctgg ccctgctcca tggtcgcctg gccggggatc
      361 cgggccagga acttgcgcag cgcctcgatc ggctgccggg actcgagcac cttgaccacc
      421 gagctgccac cgctcagccg cgccggcttg cgcgtgggag gacgcgaggg tggcttgcgc
      481 gccgatggcc tggcaggcgc gacgtgcctc gcggcgatgg tcggcgtggc ggtcgtcttc
      541 ccggcggcgg acgtggtggg gccggtcttc ttcctgctcg cgggcgtctt gctgacggcg
      601 ggcgcctgct tggcggcgct cttcctggcg gtcgacttcg ccctggcggg agtcgccctg
      661 gcgggagtcg ccctggcggg agtcgccttg gcaggagtcg cccgggcggg agccgccttg
      721 gcgcgcagct tcgcgctggc ccgggaccgg ggcgacgagc gtgcgcgtgc catcgccacc
      781 gacgctatca tgccgcgacg cgcgcgcacg cgatcgggcg cgccgtgatc gccgacgcgc
      841 gcgcgaagct tgcctccctg cgcgcgcgat cgacgctatg ttcgccgcat gcggatcctc
      901 gccgtcgccg tgatcctcgc cgcgtgcggt gacaaccgcg tcgagggccc gccgccgccg
      961 ggctgcgatg catggcacca gtggggcaac aacgcgtcgc acgacggcgc cagctgcgtg
     1021 cccgggcagc cgctgcgcac cgcgctatcc agcacggtct acgacccgtt cctcgccgac
     1081 gaggtcgccg acgcccgcgg cgtcctgctc gtgcactacc aggctccgct gatcgacggc
     1141 gacgacctct acatgatgac caagcacggc acctacacgc cgtgcaacgt gggcagcgac
     1201 ggtccgtcgt gcggcgatcc cgacgagctc caccggctcg actcgcagat ctgggcggag
     1261 cagcgcttcg tcatctcgcc gtccggcgcg ctcgcccagc agtggtcgtt cgactccgac
     1321 tggaagccgg tgcccgggat cggcttcgag ccgatgttcc agcccgcgct cgccggtggg
     1381 ctgatcgcga tcccgggcgc cggcggcgcg ctgtgggagc tcgatcggct gtccggccag
     1441 gtggtgcgtc acgtccagcc gctcggcgcc gcgatcgatc ccgacatcta cgtcgccggc
     1501 gggctcgcgg ccggccccga cggcaccctc tactacagcg cgttccggga cgtccagctg
     1561 cccgcgacgc cgcaggcctg gctcgtcgcg gtcgcgcccg acggcaccgt gcgcaccgcc
     1621 gactacgccg cgct
//
LOCUS       DASVOR010001811         3374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_194440,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001811 DASVOR010000000
VERSION     DASVOR010001811.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194440"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..544
                     /locus_tag="VF516_33665"
     CDS             74..544
                     /locus_tag="VF516_33665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112744.1"
                     /translation="MTDTPDPQSTQQAHDTPDEPAPAGEPATPPDDIAQPLASLIASI
                     RAAVTPNASAEARTAGANTCRSILTVLEAKPGQPLAAMPQRTSSTAQASPLATLLSQP
                     GLLSKLAAMSRDELMTLLKQITGAMPARPTTPTTAAPRFHLIEIPSVRRPDGDR"
     gene            595..921
                     /locus_tag="VF516_33670"
     CDS             595..921
                     /locus_tag="VF516_33670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112745.1"
                     /translation="MSNLVSSPGEGSSALAGAQCSPASPGSPPPLHVEPASPQDGILP
                     LANVEAAPTQEADRANLDQPRRLNGRDHEMLDFLVQKAIETCMPRRPEPPSPPGARQR
                     SINRKP"
     gene            1068..2942
                     /locus_tag="VF516_33675"
     CDS             1068..2942
                     /locus_tag="VF516_33675"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEX8112746.1"
                     /translation="MHAIQTGPVAIYARFSSELQNERSIEDQVRRCREFITQHGGNAQ
                     NAQVFPDFAVSGASLDRPGFEAMMVAVNAGRIKAIVTEDMSRISRDFADSAQIFKLLQ
                     FRQVPLLGVADGIDTSTKHAKLSFTVKSLVADIYLDDLRDKTLRGLEGRALDGFATGN
                     VAYGFHTVPAEPGTVGNRIAIHEGEAPIVRRIFTESRDGRSLTTIAHGLNRDGIPSPR
                     VGSKHKCFGWGASTIRAILYNERYAGVWRFKEKQWVKIPGTNRRQPRARNASEVMVQE
                     RPDLRIIDQKLWDAVQARLARLKQKYAGGRQKGGLTFKRAPYLLSGILVCAACGAPMS
                     MNGGSNGRYYRCQTNKTKGTCDNGFNVREDNARTKILAAVRGHLLSPEGVAHTRKRIA
                     EELRDYSNKIAAELRDRRERLARTEDKMRGLVDFIATGDRSEYVVSTLRDLEAFTRQE
                     RAALVELEQASREPLRLPSIDEVCAQVADLDTRLAQDPEAGREQLRRWLKDGSIRIGP
                     AKDGSIVAEGEILPLVVISDGGGRKGVTGANTPYPGGGGGIGRSRDVRHVRHSNEVAS
                     AGRRLARKWRDRGHLQHGARIHRGHAAVLGIPAPSGRQGRGPGGAPGPGGTRAVHAAG
                     "
     gene            3078..>3374
                     /gene="tnpB"
                     /locus_tag="VF516_33680"
     CDS             3078..>3374
                     /gene="tnpB"
                     /locus_tag="VF516_33680"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX8112747.1"
                     /translation="MEVYVALDPIDLRWGFDRLAGVVTERLGRGARSRAMFVFFGRRR
                     TALKVLFFDGTGMCIIYKRLDAGAFRIPEPVVTGDAARLINERAFEDLLAGIDLE"
BASE COUNT          619 a         1148 c         1094 g          513 t
ORIGIN      
        1 gtgattaccc actaccggcg cgcaccgcgc cgctgacacc caatcttcta caccccaaag
       61 aggactcgtc accatgacgg acacacccga tccacagagc acccagcaag ctcacgacac
      121 gccggacgag ccggcccccg ccggcgagcc ggccacaccg cccgatgaca tcgcgcagcc
      181 gctcgccagc ctgatcgcgt cgatccgcgc cgcggtgacc ccgaacgcgt ccgccgaggc
      241 gcgcacggcc ggcgcgaaca cgtgccgctc gatcctgacg gtgctcgaag ccaagcccgg
      301 tcaaccgctc gccgcaatgc cgcaacgaac atcgtccacc gcgcaggcgt ccccgctcgc
      361 gaccttgctc tcgcaacctg gcttgctctc caagctcgcc gcgatgtcgc gcgatgagct
      421 gatgaccttg ctcaagcaga tcaccggcgc gatgcccgcg cgaccgacca cccccaccac
      481 cgcggcgcca cggtttcacc tgatcgagat cccgtcggtg cgccgaccgg acggtgatcg
      541 atgagcgcga gcggccccgg tccgccgagg cgctccgaaa agccgaatcg tcccatgtcg
      601 aacctcgtct catcgcccgg ggagggttcg tcggcgctcg ccggcgcaca gtgctcgccc
      661 gcatcgccgg gatccccccc accgctccac gtggagcctg cgtcgccgca ggacggcatt
      721 ttgccgctcg cgaatgtcga ggctgcaccc acgcaggaag ctgatcgcgc taacctcgac
      781 cagccacgtc gcctcaacgg gcgcgatcat gaaatgctcg actttctcgt gcagaaggcc
      841 attgaaacat gcatgccacg ccgaccagaa ccaccatctc cacccggagc acgccagcgc
      901 tcgatcaacc gcaagccgta gcgaccaacg acgatcggca cccagtcacg acgagctcat
      961 ccaaattttt catctgaaat atcaagttct gccttgatct tatcctgtca gttactacta
     1021 tcgatctagt cgaacgcaag caggttgcac tctaaggata caagcccatg cacgcgatcc
     1081 agactggccc agttgctatt tacgcgcgct tctcatcaga gctgcagaac gaacgcagca
     1141 ttgaagatca ggtgcgccgt tgccgcgagt tcatcacgca gcacggcggc aacgcgcaaa
     1201 atgcgcaggt gttccccgat ttcgcggtgt ccggagccag cctggatcgc cccgggttcg
     1261 aggccatgat ggttgccgtg aacgctgggc ggatcaaggc gatcgtcacc gaggacatgt
     1321 cgcgcatctc gcgtgacttc gcagactcgg cgcagatctt caaactgctg cagtttcgcc
     1381 aggtgccgct cctcggcgtc gccgacggca tcgacaccag cacgaagcac gcgaagctat
     1441 cattcacggt caagagcctt gttgcggaca tctatctcga cgacctccgc gacaagacgc
     1501 tacgtggcct tgaaggtcgc gcgctcgatg gctttgcgac cggcaacgtc gcctatggct
     1561 tccacacagt gcccgccgaa cctggcaccg tcgggaatcg gatcgcgatc catgaagggg
     1621 aggcgccgat cgttcgccgc atcttcaccg agtcgcggga tggccgctcg ctcaccacca
     1681 tcgcgcacgg cttgaaccga gacggcatcc catcgccgcg cgtcggcagc aagcacaagt
     1741 gcttcggctg gggggcctcg accatccggg cgatcctgta caacgagcgc tatgccggcg
     1801 tgtggcgctt caaggagaag cagtgggtca agatccccgg caccaatcgg cgacagcccc
     1861 gcgcacgcaa cgcgtcggag gtgatggtcc aggagcggcc cgatctgcgg atcatcgatc
     1921 agaagctctg ggatgcggtg caggcgcggc tcgcgcgctt gaaacagaag tacgccggcg
     1981 gtcgccagaa gggcggcctg acgttcaagc gcgcgcccta cctgctgtcg ggcatcctcg
     2041 tgtgcgccgc ctgcggcgct ccgatgtcca tgaacggcgg atcgaacggg cgttactacc
     2101 ggtgccagac gaacaagacg aagggcacgt gcgacaacgg cttcaacgtc cgcgaagaca
     2161 acgcgcgcac gaagatcctg gccgcggtcc ggggtcacct gctgtcgcct gaaggcgtcg
     2221 cgcacacccg caagcggatc gcggaggagc tgcgcgacta ctccaacaag atcgcggcag
     2281 agctgcgcga tcgccgggag cggctcgcac gcacggagga caagatgcgc ggcctggtcg
     2341 acttcatcgc gaccggcgac cgctcggagt acgtggtctc caccctgcgc gacctggagg
     2401 cgttcacgcg ccaggagcgc gcggccctgg tcgagctgga gcaggcgtcg cgcgagccgc
     2461 tgcgcctgcc cagcatcgac gaggtctgtg cccaggtggc cgacctcgac acccggctcg
     2521 cccaggaccc ggaggccggc cgcgagcagc tgcgccggtg gctcaaggac ggctccatcc
     2581 gcatcggacc ggcgaaggac ggatccatcg tcgcggaggg cgagatcctg ccgctggtcg
     2641 tcatcagcga cggcggaggc cgaaaagggg taaccggtgc aaataccccg taccccggcg
     2701 ggggcggcgg gatcggacga tcccgggatg tcagacacgt cagacacagc aacgaagtgg
     2761 cgtcagcggg tcgccggctg gcgcgcaagt ggcgagaccg cggacaccta cagcatgggg
     2821 caaggattca ccgcgggcac gctgcggtac tgggcatccc ggctccgtcg ggaagacagg
     2881 ggcgggggcc cggtggtgcg cctggcccag gtggtacgcg cgccgtccac gcagcgggat
     2941 gaggtgccgc gcggcacggc cgtgatcgat ctgctcgatg cacgggtgcg aatcacggtg
     3001 gcatcggacg cggaccgcgc ggtactggcg atggtcctcg aggtgctcga tcggagggga
     3061 cggtgatccc gcacggcatg gaggtgtacg tcgcgctgga cccgattgac ctacggtggg
     3121 ggttcgatcg tttggcgggc gtggtgaccg agcgactggg gcgcggagcg cgcagccgtg
     3181 cgatgtttgt attcttcggg cggcggcgga ccgcattgaa ggttctgttc tttgacggca
     3241 ccgggatgtg catcatctac aagcggctcg atgccggggc atttcggatc ccggagccgg
     3301 tggtcaccgg cgatgcggct cgtctgatca acgagcgcgc gttcgaggat ctgctcgcgg
     3361 gcatcgacct ggag
//
LOCUS       DASVOR010001812         2814 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_77302,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001812 DASVOR010000000
VERSION     DASVOR010001812.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77302"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1363)
                     /locus_tag="VF516_33685"
     CDS             complement(<1..1363)
                     /locus_tag="VF516_33685"
                     /inference="COORDINATES: protein motif:HMM:NF014312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX8112748.1"
                     /translation="MAMLAAAGAGAGAVFGGCDSSGGDDGPGTGSAAPVLARPSKSGT
                     VAITGNDRFVAMVNPETGSISVFDTATDTRIASVKTGGEPSSIAIGPDDDTAYVANRA
                     DATVVKIAHLTTSPEVSAPVDVGSEPTGVALSPTGKRLFVAEFAEGRVSVIDTDTMKV
                     TATIAEPDHPRALAVTNNGDASDADELLVVPEFFGEPTGPEGTDTSRTGRIRLYKLSD
                     LGAAASISLAPIASGFAPTDATGTAVGPVTQTSPNQLASAIVVGSKLYATTVSASPAP
                     PFTFTVNVQPGLEVVDLQARQQDTSALGTMNLARLVKDQLPDPRTTQGAPPRNFLADL
                     IDVGFVGNDVAYVLSQGGDAIQRVVLDPSGPRLGSDNNKQIDLGVAPAGSALACQTPT
                     GLVVAHAAARAYVNCWVTRSLGVVDLASQALVKTVVSDAIADGEKDVQAGKRFYFTAR
                     GRWS"
     gene            1567..2343
                     /locus_tag="VF516_33690"
     CDS             1567..2343
                     /locus_tag="VF516_33690"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HEX8112749.1"
                     /translation="MRSTGLLVVVAGCGFHATAPPAQDPPPADAGAPDSSDVVLDAGP
                     GSDAMRSTFCAIDGIVACFEFEGDARDGSGNNLDAAATGVSFHPGEAGMAMQVDGSSS
                     ATVMPTILDNVDAVTIEAWIRPTQLPANTKTFDLLDVDQQYAFALNDDGTLTCDLHPN
                     PGKVSTTGPTGTVATNQWTHVACTYDGVNTSRIYINGAIAATHTGNGTLGKGGHGIGI
                     AQNYPSGSQLIGLIDQLRLLNIARSTADLCTDAGALCISL"
     gene            complement(2347..>2814)
                     /locus_tag="VF516_33695"
     CDS             complement(2347..>2814)
                     /locus_tag="VF516_33695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112750.1"
                     /translation="GAAIDHVRDDWTRGIATPLSLEPLHVAGIPGEEGGGLLYHRPTN
                     PERRGADSSWQLTRDRPRRFDPRTLPPGLTQIAGHTGHNKCLAELDGWATAAARGRRH
                     GGIRTLRHAGQTVTYDLGVTPPAGGVADMILIDGELRRVPAAEFALLPLAGLR"
BASE COUNT          371 a         1010 c         1024 g          409 t
ORIGIN      
        1 tcgaccaccg gccgcgcgcg gtgaagtaga accgcttgcc ggcctggacg tccttctcgc
       61 cgtcggcgat cgcgtcggac accacggtct tgaccagcgc ctggctcgcg aggtcgacga
      121 cgccgaggct gcgcgtgacc cagcagttga cgtaggcgcg ggccgcggcg tgcgcgacga
      181 ccaggccggt cggggtctgg caggccagcg cgctgccggc cggcgcgacg ccgaggtcga
      241 tctgcttgtt gttgtccgag ccgagcctgg ggccgctcgg gtcgagcacg acgcgctgga
      301 tcgcgtcgcc gccctgcgac agcacgtagg cgacgtcgtt gccgacgaag ccgacgtcga
      361 tcaggtcggc gaggaagttg cgcggcggcg cgccctgcgt ggtcctggga tccggcagct
      421 ggtccttgac cagcctggcg aggttcatcg tgccgagcgc gctggtgtcc tgctgccggg
      481 cctggagatc gacgacctcg agccccggct ggacgttgac ggtgaacgtg aacggcggcg
      541 ccggcgacgc cgacaccgtg gtcgcgtaga gcttgctgcc gacgacgatc gccgacgcga
      601 gctggttggg cgaggtctgg gtcaccgggc cgaccgcggt cccggtggcg tcggtcggcg
      661 cgaaccccga cgcgatcggc gcgagggaga tcgacgccgc cgcgccgagg tccgacagtt
      721 tgtacagccg gatccggccg gtccggctgg tgtcggtgcc ctccgggccg gtcggctcgc
      781 cgaagaactc cggcacgacc agcagctcgt cggcgtcgct cgcgtcgccg ttgttggtca
      841 ccgcgagcgc cctggggtga tcgggctcgg cgatcgtcgc ggtgaccttc atcgtgtcgg
      901 tgtcgatcac gctgacccgg ccctccgcga actcggcgac gaacagccgc ttgccggtcg
      961 gcgacagcgc cacgccggtc ggctccgagc cgacgtcgac cggcgcgctg acctcggggc
     1021 tggtggtcag gtgggcgatc ttgacgaccg tcgcatcggc gcggttggcg acgtatgcgg
     1081 tgtcgtcgtc ggggccgatc gcgatcgagc tcggctcccc gccggtcttg acgctggcga
     1141 tccgggtgtc ggtcgcggtg tcgaacaccg agatcgatcc ggtctcgggg ttgaccatcg
     1201 cgacgaacct gtcgttgccg gtgatcgcca cggtccccga cttcgagggc cgggcgagca
     1261 cgggcgcggc gctgccggtg ccgggtccgt cgtcgccgcc gctgctgtca catccgccga
     1321 acacggcgcc ggctccggcg ccggccgccg cgagcattgc catccaggaa tcgcgtgtcg
     1381 tcatcttagt aaaggctcct tccggggagc cttcgatagc gacctgcgtg ccacgttctg
     1441 cggcggtgcc gccggacggc ggtccagcta gttgcgccgg ctgcacagga tccgcatcac
     1501 acggcgctcg ctggccggcc tggccgggcc agcagcgggt gccagatcgt gattaagctc
     1561 gccgccatgc gcagcactgg cttgctcgtc gtcgtggccg ggtgcgggtt tcacgccacc
     1621 gcgcctcccg cgcaggaccc gccgccggcc gacgccggcg cgccggacag ctccgacgtc
     1681 gtgctcgacg ccggcccggg ctccgatgcg atgcggtcca cgttctgcgc gatcgacggc
     1741 atcgtcgcct gcttcgagtt cgagggcgac gcccgggacg gcagcggcaa caacctcgac
     1801 gccgcggcga ccggcgtgtc gttccacccc ggcgaggccg gcatggcgat gcaggtcgac
     1861 ggcagcagct ccgcgaccgt catgccgacg atcctggaca atgtcgacgc ggtgacgatc
     1921 gaggcgtgga tccggccgac ccagctgccg gccaacacga agaccttcga cctcctcgac
     1981 gtcgaccagc agtatgcgtt cgcgctcaac gacgacggca cgttgacgtg cgacctgcac
     2041 cccaacccgg ggaaggtctc gacgaccggg cccaccggca ccgtggccac caatcagtgg
     2101 acgcacgtcg cgtgcaccta cgacggcgtc aacacctccc gcatctacat caacggcgcc
     2161 atcgccgcca cgcacaccgg caacggcacg ctcggcaagg gcggccacgg gatcgggatc
     2221 gcgcagaact atccgtcggg ctcgcagctg atcggcctca tcgatcagct ccggctgctc
     2281 aacatcgcgc gatccacggc cgacctctgc accgacgccg gcgcgctctg catctcgctg
     2341 tgacgctcag cgcagcccgg cgagcggcag cagcgcgaac tcggcggccg ggacccggcg
     2401 cagctcgccg tcgatcagga tcatatcggc gacgccgccg gccggcgggg tcacgccgag
     2461 atcgtaggtc acggtctggc cggcgtgccg cagggtccgg atcccaccgt gccggcggcc
     2521 gcgcgccgcg gcggtggccc agccgtcgag ctcggccagg cacttgttgt ggccggtgtg
     2581 gccggcgatc tgggtcaggc cgggcggcag ggtgcgcgga tcgaaccgcc gcggccggtc
     2641 gcgcgtgagc tgccaagacg agtcggcgcc gcgccgctcg gggttggtcg gccggtggta
     2701 cagcagcccc ccgccctcct cgccggggat cccggcgacg tgcagcggct cgagcgacag
     2761 cggggtcgcg atgccgcgag tccagtcgtc gcgcacgtgg tcgatcgccg cgcc
//
LOCUS       DASVOR010001813         1576 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_486656,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001813 DASVOR010000000
VERSION     DASVOR010001813.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_486656"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..639)
                     /locus_tag="VF516_33700"
     CDS             complement(<1..639)
                     /locus_tag="VF516_33700"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8112751.1"
                     /translation="MCPLGEARRGAMYLAEHPVLHTRRAVRVLLPPLARDPRAVQRFV
                     DVARAAARVQHRNLVQVHDVGPLAGGAWFMTLDHIDGGTLDRQIASHAGPMALDPIAR
                     IVGEIAAGLQAAHDHGIVCGRLTPDHVGLTTRDGDPRCVVQLDGVCGIGSWEARIDDE
                     PPDPDPALPYRSPEQLRGAPPGAAADVFALGVIAYRMTTGGWFPPPCGDSRSE"
     gene            1290..>1576
                     /locus_tag="VF516_33705"
     CDS             1290..>1576
                     /locus_tag="VF516_33705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8112752.1"
                     /translation="MVHGRGSSTTRPSGDRFRGLLEAAPDAMVIVGEDGRIALVNGQA
                     ERLFGYTRDELYAQPVELLVPERFQSGHPTHRADYVHDARPRPMGAGLDLAG"
BASE COUNT          250 a          566 c          537 g          223 t
ORIGIN      
        1 ctcgctccgc gagtcaccgc acggaggcgg gaaccatccg ccggtggtca tccggtacgc
       61 gatcacgccg agcgcgaaca cgtcggccgc agcgccgggc ggcgcaccgc gcagctgctc
      121 gggcgaccgg tacggcagcg ccgggtcggg atcgggcggc tcgtcgtcga tccgggcctc
      181 ccaggagccg atgccgcaca cgccgtcgag ctgcacgacg cagcgcggat cgccgtcgcg
      241 cgtggtcagg ccgacgtggt cgggcgtgag cctgccgcag acgatgccat ggtcgtgcgc
      301 ggcctgcagg ccggcggcga tctcgcccac gatgcgagcg atcgggtcca gcgccatcgg
      361 tccagcgtgg ctggcgatct ggcggtcgag cgtgccgccg tcgatgtggt cgagcgtcat
      421 gaaccaggcg ccaccggcga gcggaccgac atcgtggacc tggaccaggt tgcgatgctg
      481 cacccgtgcg gccgcgcgtg ccacgtcgac gaagcgctgc acggcgcgcg gatcccgggc
      541 cagcggtgga agcagcacgc gcaccgcgcg ccgggtatgc agcaccgggt gctcggccag
      601 gtacatcgcg ccgcgacggg cctcccccag cggacacacg atcgtgaacg ccccgacccg
      661 cactccgatc agactgctcg cctcgcctga tcccaggcgt cgatcatcac ccaaccgcat
      721 gcgcagtgct cgcatccctc ggaacaaggc tataccatct gctagcacta aatccaagga
      781 gtcataccca ccaattgtgg gtgatctcga agatttagtt tctggatcag cgactcgcgc
      841 gatgcttcaa cggcaccgga tcgtgtcgtc cggattccgg acatcggaat cggatgtccg
      901 caggacggac atcctggcac ccatcgtccg ggtcacggac gctccggccg gccggcgatc
      961 gcacgtcggc cagggcattt tgccccagcg ccgggccctg ggctgccgcg atgcgccaca
     1021 cgccgcgcgg aagcacgcgc ccgccgcccg atgtcggtgc acaagcaccg tgcgcgaacg
     1081 tccgtgccgc aagatcggcg gcgctcctac gaaaattcct catcgatctc gacccgtccg
     1141 ggcgaccgcg atgaaccagg cgttagatcg cagacgaccg atggcaacgc cgcggggttc
     1201 tgcgatcatg gtcgacagtc gcgcgcttca ctctccgcgc cgtagaggtg aagccgtgtg
     1261 ttgctggccg cggcgcagac agggtgttga tggtccacgg tcgtggatcg agtacgacga
     1321 gaccatcggg cgaccggttc cgcggcctgc tggaggccgc gcccgatgcg atggtgatcg
     1381 tcggcgagga cggccggatc gcgctggtca acggccaggc cgagcgactg ttcggctaca
     1441 cccgcgacga gctctacgcc cagcccgtcg agctcctcgt ccccgagcgg ttccagtccg
     1501 gccaccccac gcaccgcgca gactacgtcc acgacgcgcg gccccggccg atgggcgccg
     1561 ggctcgacct ggccgg
//
LOCUS       DASVOR010001814         2707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_575094,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001814 DASVOR010000000
VERSION     DASVOR010001814.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2707
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575094"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VF516_33710"
     CDS             <1..224
                     /locus_tag="VF516_33710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112753.1"
                     /translation="IELVPITVAVANLMRKGDLQMLPSAIQTGRDAGMIPLERSLARL
                     LETGVVAPQVVKAIAADHDLLAALSARLR"
     gene            916..1488
                     /locus_tag="VF516_33715"
     CDS             916..1488
                     /locus_tag="VF516_33715"
                     /inference="COORDINATES: protein motif:HMM:NF015928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase inhibitor family I36 protein"
                     /protein_id="HEX8112754.1"
                     /translation="MSKIMHALFIASLVVSATACAMDGQDEQGSLSETSESAALPAPS
                     IDAAQQAGWELLDATELPVDLPDVDSPDGDSSRACTVITSGVGGACPRGFACLYQNSN
                     RKGLGVGISAGCGSSDLRAVRCPSCTNGIHGNNGTFNDQMSSWHNASGRRYCWYFDIN
                     FRGERHTMGNGVIQNVLPRENDRASSFKPC"
     gene            complement(1580..>2707)
                     /locus_tag="VF516_33720"
     CDS             complement(1580..>2707)
                     /locus_tag="VF516_33720"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8112755.1"
                     /translation="RVVLVAAGSDGIALAARCGAADVVMRPFSASELAFRIGRVPAGD
                     LRGDKRRPRKSDIIIGTGPWIKELYDRIAMVAATDVTVAIFGESGTGKELVARTIHTS
                     SPRHDAPFVVVNCAAIPEALLEDELFGHVRGAFTDATRDREGLLAAAHTGTLFLDEIG
                     ELPLVLQGKLLRVLQSQEFRRIGDDQDRRVDVRIVTATNRDLDQLVARGSFRQDLYYR
                     INVFPMHLPPLRDRPEDIALLVHHFISKYRGRLGKPIDGIAPGALRRFAAYDFPGNVR
                     ELENKVHQAMVVAEGPLIGEADVALPAGRSAGGRPDITRPFRELKQEAIDAFEKAYLT
                     ELLREHRGNLARAARAAGMDRKNLWTLVARHGLDRDQFRKP"
BASE COUNT          426 a          924 c          916 g          441 t
ORIGIN      
        1 cgatcgagct cgtgccgatc accgtcgcgg tcgccaacct gatgcgcaag ggcgacctcc
       61 agatgctgcc gtccgcgatc cagaccggcc gcgacgccgg catgatcccg ctcgagcgct
      121 cgctcgcccg cctgctcgag accggcgtgg tcgcgcccca ggtcgtcaag gcgatcgccg
      181 ccgaccacga tctgctcgcc gcgctatcgg cgcgcctgcg ctagaggtgg aaccggcgag
      241 ccggcgagcc ccatcggatg ccggcggtgt aacgggcgtg cgttgaatcg acggctctac
      301 aacaggacca acccgacagc accgacgagc atgtcgtgtc ctcgccgtgt acgacccgta
      361 gccggcatca cgcgttccac gagcaagcag gccagggccc ggcggtgcca tctcgaacgg
      421 cccccggccc cagatgccat caagcgcgct gggagctctc cggcaggccg acgcccgtcg
      481 ccgccgagga tcgctcgcgg tgttagcccc cgggccgctc ggcccgcccc cgcctcgcac
      541 accgcccggc agcgcacacg gtcgtttgct gctctgccgt aatctggaac acccgatctg
      601 cggtcgagag gttctcgctc gtccggtggt tgcgccgcct ctgaatcccc gatcggtcct
      661 gccggccgtg gcgagctcga caaccgtcga cgatcactgc cgaagcgatc gtaggacatg
      721 cactgccatg gaatgcgatc gtacacatca catctcgagg atgcagtgct gtagcgcgga
      781 tcgtcaaact gcctcgttat tcttcatgct gccgagtttg tccgtgctcg tgacgccggc
      841 gaaccccgga tcggagattg caactccaag tcatctattg ttggcccaat atatgccaca
      901 caaggagata atgacatgtc aaagatcatg catgcgctgt tcattgcaag tctggtagtc
      961 tcggctaccg cttgcgccat ggacggtcag gacgagcaag gctctctctc cgaaacctcc
     1021 gagagcgcag cgctcccggc gccaagcatc gacgctgccc agcaagcagg gtgggaattg
     1081 ctcgacgcga cggagctgcc cgtggacttg ccggacgtcg actcgccgga cggagactca
     1141 tcgcgggcct gcacggtgat caccagcggg gtcggcggcg cgtgcccgcg tgggttcgcc
     1201 tgtctatatc agaactccaa tcgcaagggg ctcggggttg gcatctcggc tggatgtggc
     1261 agcagcgatc tgcgcgcagt tcgttgtccc agctgcacga acgggatcca cggtaacaat
     1321 ggaactttca acgatcagat gtcgtcctgg cacaacgcca gcggccggcg ttattgttgg
     1381 tatttcgaca ttaacttccg gggagaacgt cacacgatgg gcaatggcgt cattcagaac
     1441 gtccttccgc gcgagaacga ccgggcgtcg tccttcaagc cgtgctgatg tagcggttgc
     1501 agcgagctca ccgtctcggt gcgcgagctc gttcgcgcgc gactcgaagg gaacggcaac
     1561 cgggagctat cgcgatccgc tacggcttgc ggaactggtc gcggtcgagg ccgtggcgcg
     1621 cgaccagcgt ccacaggttc ttgcggtcca tgcccgcggc gcgcgcggcg cgcgcgaggt
     1681 tgccgcggtg ctcgcggagc agctcggtca ggtaggcctt ctcgaacgcg tcgatcgcct
     1741 cctgcttgag ctcgcggaac ggccgcgtga tgtcgggccg gccgccggcg ctgcggcccg
     1801 cgggcagtgc gacgtcggcc tcgccgatca gcgggccctc ggcgaccacc atcgcctggt
     1861 ggaccttgtt ctcgagctcg cggacgttgc ccggaaagtc gtacgccgcg aaccggcgca
     1921 gcgcgcccgg cgcgatgccg tcgatcggct tgccgagccg gccgcggtac ttgctgatga
     1981 agtggtggac caggagcgcg atgtcctcgg gtcgatcgcg cagcggcggc aggtgcatcg
     2041 ggaacacgtt gatccggtag tacaggtcct ggcggaagct gccgcgcgcg accagctggt
     2101 cgaggtcgcg gttggtcgcc gtgacgatcc ggacgtcgac ccggcggtcc tggtcatcgc
     2161 cgatccgccg gaactcctgc gactgcagga cgcgcagcag cttgccctgc agcaccagcg
     2221 gcagctcgcc gatctcgtcg aggaacagcg tgccggtgtg cgcggcggcg agcaggccct
     2281 cgcggtcgcg ggtcgcgtcg gtgaacgcgc cgcggacgtg gccgaacagc tcgtcctcga
     2341 gcagggcctc ggggatcgcc gcgcagttga ccacgacgaa cggcgcgtca tggcgcggcg
     2401 acgaggtgtg gatcgtgcgc gcgaccagct ccttgccggt gccgctctcg ccgaagatcg
     2461 cgacggtgac gtcggtcgcg gcgaccatcg cgatccggtc gtacagctcc ttgatccagg
     2521 gcccggtgcc gatgatgatg tcggacttgc ggggccgccg cttgtcgccg cgcagatcgc
     2581 cggccggcac ccggccgatg cggaacgcga gctcggaggc gctgaacggc cgcatcacca
     2641 cgtcggccgc gccgcaccgc gccgccagcg cgatgccgtc gctgccggcg gcgaccagca
     2701 cgacgcg
//
LOCUS       DASVOR010001815         4413 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_48473,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001815 DASVOR010000000
VERSION     DASVOR010001815.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4413
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48473"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..1082
                     /locus_tag="VF516_33725"
     CDS             138..1082
                     /locus_tag="VF516_33725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112756.1"
                     /translation="MQASRHNGPAGRITTMLCALAFTGCAVSDEPADEPTGETSSAIS
                     MAGGATWNGTSPLTLGNLNQETCFLSGISGSFIGQPYYYFNNGTTFHPAEVKVFPDFA
                     SGKWKMVTDWGSGTGVQAGVVCINIPYTSSAVHEFSWGNNVTSTGYIGTASTHCFLRD
                     VWATSGLDGDTFNGATLTNLTITRTALASGAVAFDMNSSYVHNLVGDTGWGGATAVCV
                     DYAAIGLWGYRFIGPTNSTSSATSTGFLRDFYPNGPAVATANTLCSITGVRGRWINPS
                     PDPIGINDGVVLINDPAKTSFWEVTTSNGRTADVACVR"
     gene            complement(1089..1640)
                     /locus_tag="VF516_33730"
     CDS             complement(1089..1640)
                     /locus_tag="VF516_33730"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEX8112757.1"
                     /translation="MPRLRVPVTDRDHQRGPARASVTLVEYGDFQCPFCRQAFYALRN
                     IEDRFQNDLRLVYRHFPLAEIHPLALIAAEASEAAGAQGRFWEMHDTLFQNQPNFQPE
                     ELIGYASQLGLDVDAFTEDLMALRHRDRIREDFMGGVRSGVNGTPSLFINGELFDAPA
                     EERLLARAIEAARRGWQGAAVPQ"
     gene            complement(1633..2199)
                     /locus_tag="VF516_33735"
     CDS             complement(1633..2199)
                     /locus_tag="VF516_33735"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEX8112758.1"
                     /translation="MEPAQTQNTTQTGPDRRTPGTLPTAPLAAGQKAPDFELHTTPDQ
                     RVALSSFVGRPIVLVFYPADWSPVCGDQLALYNELHGEWQKHRAQLLGISVDNTWCHI
                     AYAKDRRLRFPLLSDFEPKGDVARRYGAYDHDQGTATRALFVLDREGMIAWSYVSPVG
                     VNPGADGILEALEALDRRTGPRQERAHA"
     gene            complement(2275..3414)
                     /gene="tal"
                     /locus_tag="VF516_33740"
     CDS             complement(2275..3414)
                     /gene="tal"
                     /locus_tag="VF516_33740"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF002881.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEX8112759.1"
                     /translation="MTPNRLLALVELGQSIWIDDLDRMRIVSGELRRHIVEDGLRGVT
                     SNPTIFDKAIASPSYDAPIRVLALRGKQPAEIYEALAVEDIQLAADELRRLFDQLDGR
                     DGFVSLEVSPHLAYDTLGTVDEARRLWRAVDRPNVMIKVPGTEPGLAAFEALIAEGIP
                     INVTLLFGLDRYEQVAYAYLAALEARARRGLPVRVPSVASFFLSRIDAAIDPELDRRA
                     RDRRIPADLAARLRGRSAIASARLAYQRYKRIVGEPRFRALEGLGARPQRLLWASTST
                     KDPAYSDVYYVDALIGPDTIDTMPLETFEAYRVHGNPAVRLEDRLDEARAVLDGLKTA
                     GIDLDAVTRRLEQEGVQKFSASYDRLIAAIAARRDAALAHAHLGG"
     gene            complement(3448..3888)
                     /locus_tag="VF516_33745"
     CDS             complement(3448..3888)
                     /locus_tag="VF516_33745"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEX8112760.1"
                     /translation="MRLISPAFRDGGEIPEKYAKDGEDRSPPLTWSDVPAEAVSLALI
                     VDDPDAPRGTWTHWVVTDLPARTTGLQEGATAGHVGLNDWKHAAWNGPAPPQGRHRYE
                     FKLFALDRELGLSRPTRHEVERAMAGHVLAEAKLTGTYQAERAA"
     gene            4171..>4413
                     /locus_tag="VF516_33750"
     CDS             4171..>4413
                     /locus_tag="VF516_33750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112761.1"
                     /translation="MPSHAGAWTRPAAVFRAHLPRWARPDVRPVERGGRRDDRRGPAT
                     HEHHRPWYIRSVASKFLDAAARAAFPRAVEAIEQVSA"
BASE COUNT          642 a         1583 c         1512 g          676 t
ORIGIN      
        1 ccttgcccac gtcgaccgtc gactacccgc gctgagcggc ggctacccgg cctcggcggt
       61 caccgacccg cgcggcccgc gcgggaagat ccggcgcgac cggcgtttcc tcggcgaaca
      121 ggggggacac gatgttcatg caagcttcac gtcacaacgg tccggcgggc cggatcacca
      181 ccatgctatg tgcgctggcg ttcaccggct gcgcggtctc cgacgagccc gccgacgagc
      241 ccacgggcga gaccagctcg gcgatctcga tggcaggcgg cgccacgtgg aacggcacca
      301 gtccgctgac gctcggcaac ctcaaccagg agacctgctt cctcagcggg atctccggct
      361 cgttcatcgg ccagccgtac tactacttca acaacggcac gacgttccat cccgccgagg
      421 tcaaggtgtt tccagacttc gcgtccggca agtggaagat ggtgaccgac tggggcagcg
      481 gcaccggcgt gcaggccggc gtggtctgca tcaacatccc gtacacctcg agcgcggtcc
      541 acgagttctc gtggggcaac aacgtcacga gcaccgggta catcgggact gccagcacgc
      601 actgcttcct gcgcgacgtg tgggcgacct ccggcctcga cggcgacacg ttcaacggcg
      661 cgacgctgac caacctgacg atcacccgga ccgcgctggc gtcgggcgcg gtcgcgttcg
      721 atatgaacag cagctacgtc cacaacctgg tcggcgacac cgggtggggc ggcgccaccg
      781 cggtgtgcgt cgactacgcg gcgatcggcc tgtggggcta ccgcttcatc ggcccgacca
      841 acagcacgag cagcgcgacg tcgaccggct tcctgcgcga cttctacccc aacggcccgg
      901 cggtcgcgac ggcgaacacg ctgtgctcga tcaccggcgt gcgcggccgc tggatcaacc
      961 cgagcccgga cccgatcggg atcaacgacg gcgtcgtcct gatcaacgac ccggccaaga
     1021 ccagcttctg ggaggtcacc accagcaacg ggcgcaccgc cgacgtcgcg tgcgtccggt
     1081 agcgagcctc actggggcac ggcggcgccc tgccagcccc gccgcgccgc ctcgatcgcg
     1141 cgcgcgagca gccgctcctc ggcgggcgcg tcgaacagct cgccgttgat gaacagcgac
     1201 ggtgtgccgt tcacgccgct gcgcacgccg cccatgaagt cctcgcggat ccgatcgcgg
     1261 tggcgaagcg ccatcagatc ctcggtgaac gcatcgacgt cgaggccgag ctggctggcg
     1321 tatccgatca gctcctcggg ctgaaagttg ggctggttct ggaacagcgt gtcgtgcatc
     1381 tcccagaacc tgccctgagc gcccgccgcc tcggacgcct ccgcggcgat cagcgccagc
     1441 gggtggatct cggcgagcgg gaaatgccgg tagaccaacc ggagatcgtt ctggaatcgg
     1501 tcttcgatgt tgcgcagggc atagaatgcc tgtctgcaga acggacactg gaagtcgccg
     1561 tactcgacca gggtgaccga cgcgcgcgcc ggaccccgct gatggtcgcg atcggtcacc
     1621 gggacgcgca gcctaggcat gggctcgctc ctgtcgcggc ccggttcgtc gatcgagcgc
     1681 ctcgagcgcc tcgaggatgc cgtcggcccc cgggttgacg ccgaccggcg acacgtagct
     1741 ccacgcgatc atgccctcgc ggtcgagcac gaacagcgcc cgcgtcgcgg tgccctggtc
     1801 gtggtcgtag gcaccgtagc gccgtgcgac gtcgcccttc ggctcgaagt cggacagcag
     1861 cgggaaccgc agccggcgat ccttcgcgta cgcgatgtgg caccacgtgt tgtcgacgga
     1921 gatacccagc agctgtgcgc gatgcttctg ccactcgccg tgcagctcgt tgtagagcgc
     1981 gagctgatcc ccgcacaccg ggctccagtc cgcgggatag aacaccagca cgatcgggcg
     2041 cccgacgaag gacgacagcg cgacgcgctg atcgggggtg gtgtgcagct cgaagtccgg
     2101 ggccttctgt ccggcggcga gcggcgcggt gggcaacgtg cccggcgtcc ggcggtcggg
     2161 accggtctgg gtcgtattct gggtttgcgc tggttccatg gctacctctg ttcgagtggt
     2221 gtggtggaaa ctggctgcaa gcgccggacc gccgggccgt ccggccgccg gacgtcatcc
     2281 cccgaggtgc gcgtgcgcga gcgccgcgtc tcgccgggca gcgattgcgg cgatcagccg
     2341 atcgtaggac gccgagaact tctgcacgcc ttcctgctcc aggcgccggg tcaccgcatc
     2401 gagatcgata ccggcggtct tcaggccgtc gagcaccgcg cgcgcctcgt ccaggcggtc
     2461 ctccaggcgc accgcgggat tgccgtgcac gcggtaggcc tcgaacgtct ccagcggcat
     2521 ggtgtcgatc gtgtcggggc cgatcagcgc atcgacgtaa tagacatcgc tgtaggccgg
     2581 atccttcgtg ctcgtgctcg cccacagcag gcgctggggc ctggcgccga gcccctcgag
     2641 cgcgcggaac cgcggctcgc cgacgatccg cttgtagcgc tggtaggcca ggcgagcgct
     2701 cgcgatcgcg gaccggccgc gcagccgggc ggccaggtcc gccgggatcc ggcggtcgcg
     2761 cgcccggcgg tccagctccg ggtcgatcgc ggcgtcgatc cggctcagga agaagctcgc
     2821 gaccgacggc acccgcaccg gcagcccgcg acgcgcgcgc gcctcgagcg ccgccaggta
     2881 ggcgtacgcg acctgctcgt agcggtccag cccgaacagc aaggtgacgt tgatcggaat
     2941 cccctcggcg atcagcgcct cgaacgccgc caggccgggc tcggtccccg ggaccttgat
     3001 catgacgttc ggccggtcga cggcccgcca cagccggcgc gcctcgtcga cggtccccag
     3061 ggtgtcgtag gccaggtgtg gcgagacctc caggctgacg aagccatcgc gcccgtcgag
     3121 ctgatcgaac agccggcgga gctcgtcggc ggcgagctgg atgtcctcga cggcgagcgc
     3181 ctcgtagatc tcggccggct gcttgccgcg cagcgcgagc acgcggatcg gcgcgtcgta
     3241 gctcgggctg gcgatcgcct tgtcgaagat cgtcgggttc gaggtcacgc cgcgcagccc
     3301 gtcctcgacg atgtggcggc gcagctcgcc cgacacaatc cgcatgcgat ccaggtcatc
     3361 gatccagatg ctctggccta gctcgacgag cgcgagcagt cggttcggtg tcatggcgtc
     3421 attccccctg ctctacgcgg ccctggccta cgcggcccgc tccgcctggt acgtgccggt
     3481 cagcttcgcc tcggccagca cgtggccggc catcgcgcgc tcgacctcgt gccgcgtcgg
     3541 ccgcgacagc ccgagctcgc gatcgagcgc gaacagcttg aactcgtagc gatgccggcc
     3601 ctgcggcggc gcgggcccgt tccaggcggc gtgcttccag tcgttcaagc cgacgtgacc
     3661 cgccgtcgcg ccctcctgga ggcccgtcgt cctcgccggc agatcggtga cgacccagtg
     3721 cgtccaggtt ccacgcgggg catctggatc gtcgacgatc agcgcgaggc tgaccgcctc
     3781 cgccgggacg tcgctccagg tgagcggcgg cgagcgatcc tcgccgtcct tggcgtactt
     3841 ctcggggatc tctccgccgt cgcggaatgc cggggatatc aatcgcatgg tccctccctc
     3901 ccgatcgcgg ccgatcgcgg ccgacgatgg cgcgcgctcg atggctcgag gggatcacca
     3961 gatgtgcagc ggctcttcaa ggtctcaccc gcagagcggt tccgatcctt gcggaccgat
     4021 gcccctcctg tcgtttgctc gtcagccgac tccaggggtt tgtcccgatc tgcgagtcgc
     4081 ctccggacgg gcattccctt cttgagcttt tctctcgcac ctggtgatcc gctccagcca
     4141 tcgaacacga ccggcggtgg agcaaccgcc gtgccctcgc acgccggcgc gtggacgcga
     4201 cctgcggcgg tgttccgcgc gcacctgccg cggtgggcga ggccggacgt gcgcccggtc
     4261 gagcgcggtg gccggcgcga cgatcgccga gggccggcga cgcacgagca tcaccggccg
     4321 tggtacatcc gcagcgtggc cagcaagttc ctcgatgccg ccgcgcgcgc cgcgttcccc
     4381 cgcgcagtcg aggcgatcga gcaggtgtcg gcg
//
LOCUS       DASVOR010001816         2629 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_281017,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001816 DASVOR010000000
VERSION     DASVOR010001816.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281017"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(404..652)
                     /locus_tag="VF516_33755"
     CDS             complement(404..652)
                     /locus_tag="VF516_33755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112762.1"
                     /translation="MKPLGRAAPRYFSSPHSGRRHFKQTGSDPKAQHMFFETWLWPGG
                     ETFDTSRSILFVVQTSLSTMKNSSHDEERMIRRGYTVP"
     gene            708..2060
                     /locus_tag="VF516_33760"
     CDS             708..2060
                     /locus_tag="VF516_33760"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8112763.1"
                     /translation="MATSSLALAYAGCVASNDAKDGSDEPVAAAEQAVTAIGDPLPGT
                     DPDKFAEAKANFAAEEEITDGLGPVFNERGCGNCHTTPVVGGSGVQIERRFGKVTNGV
                     FFAYDRAPDNEGGTLRQLFSNGTYVNPATGKTCTIPVEHEPADANVHNVGRRTLPLFG
                     LGLVDAMPDGFFDFLALTEPAAQRGTVLRAIPQFPDPRDPNQSFTRTRVARFGIKDQQ
                     TNLQSFAGDAYINEMGISTQSCFRGQSVTAFATDNQSNNVAPPAGCNMGDLAPQQPDG
                     SFTDDAVGPCVVNGQAISEVQDDLDNFRFFMEHEAPPPQVTSDPIGFAAGKAVFQAIG
                     CGNCHTTTIFVTPANPFNGVPGNFAFSPFSDFLVHDMGSLGDGIGNTGDPVAVTRQMR
                     TAPLWGARFNTQFLHDGRAKTVRAAILAHDGQGFAARFAFAVLDPISQALLLEFVNSI
                     "
     gene            complement(2057..>2629)
                     /locus_tag="VF516_33765"
     CDS             complement(2057..>2629)
                     /locus_tag="VF516_33765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112764.1"
                     /translation="AVDALFLAVPAAAAPAVLDTARGLPAGTIVVDCTNPLRWDDGPV
                     HTPPPEGSMAAHLAKRFPALRLIKAFNTFGAEFHDRPELGSAAADLYLAGDDAGARRG
                     IAELARTLGYDPVDVGPLRNAAHLESLAILDPPRHRRQARPRVRVQGPGALKRCRRFR
                     RSVPACMDVRWRALAGERSEALRRRAVVQA"
BASE COUNT          443 a          875 c          893 g          418 t
ORIGIN      
        1 ccgccggcga gccggtcgtc gtccgcgccg gtgcgagtgc accgagcgag cccgacgccg
       61 gctggcagcg ttgcctggcg gatgtcttca cgctggccca gcgactggcg aagtgaaggc
      121 gggcgacgcg agctgctcta ctgtgacctc aggtcgagag ttcagacgcg cgggctgatg
      181 gttgttaaca tgagctgtag caactggcgc tgggaccgcg cagcgcaaga tgctgatcgc
      241 aacgtgtcct gtcgcaaacc gtttcgagaa aattgcggga tcggtcgatt cgcgctcgtg
      301 ctcctcatcg gaaattcatc gcgacgtgcg gtgactcaac ctgattggca aataggtggc
      361 ctggcatcga gcatctcgaa tgagatcatg aataagttgt ccatcagggc acggtatacc
      421 ctcgacgaat catgcgttct tcgtcgtggc tggaattctt cattgtgctg agtgaggtct
      481 gcaccacaaa caggatcgaa cgagaagtgt caaaagtttc accgccaggc cacaaccacg
      541 tctcgaaaaa catatgttgt gctttggggt cgcttcctgt ttgcttgaag tgtcgccgtc
      601 ccgaatgcgg cgagctgaag taccgaggcg cagcgcggcc gagcggtttc acgctgcgaa
      661 cctccattct gccgaggagg aatgtgatgc aacgatactt gatgtctgtg gcgacgagct
      721 cactcgccct ggcgtatgcc gggtgcgtcg cgtcgaacga cgcgaaggac ggcagcgacg
      781 agccggtcgc ggcggccgag caggcggtga ccgcgatcgg cgacccgcta ccagggaccg
      841 atccggacaa gttcgccgag gccaaggcca acttcgcggc cgaggaggag atcaccgacg
      901 gcctcggccc cgtgttcaac gagcggggct gcggcaactg ccacaccaca ccggtggtcg
      961 gcggctcggg cgtccagatc gagcgccggt tcggcaaggt caccaatggc gtgttcttcg
     1021 cctacgaccg tgcgccggac aacgagggcg gcacgctgcg ccagctgttc tcgaacggta
     1081 cctacgtgaa cccggcgacg gggaagacct gcacgatccc ggtcgagcac gagccagcgg
     1141 acgccaacgt ccataacgtc ggccggcgca cgctgccgct gttcggcctc ggcctggtcg
     1201 acgcgatgcc cgacggcttc ttcgacttcc tggccttgac cgagccggcg gcccagcgcg
     1261 gtacggtgct gcgcgcgatc ccgcagttcc ccgatccgcg cgatccgaac cagagcttca
     1321 cgaggacccg cgtggcgagg ttcggcatca aagaccagca gaccaacctc cagtcgttcg
     1381 ccggcgacgc gtacatcaac gagatgggaa tctcgacgca gagctgcttc cgcggccagt
     1441 cggtcaccgc gttcgcgacc gacaaccagt cgaacaacgt tgcgcccccg gccggctgca
     1501 acatgggcga cctggcgccg cagcagccgg atggcagctt caccgacgac gcggtcggcc
     1561 cgtgcgtggt caacggccag gcgatcagcg aggtccagga cgatctcgac aacttccggt
     1621 tcttcatgga gcacgaggcg ccgccgccgc aggtcacgtc cgatccgatc gggtttgccg
     1681 ccggcaaggc cgtgttccag gccatcggtt gcggcaactg ccacaccacg accatcttcg
     1741 tgaccccggc gaatccgttc aacggcgtgc ccggcaactt cgcgttctcc ccgttctcgg
     1801 acttcctggt ccacgacatg ggctcgctcg gtgacggcat cggcaacacc ggcgatccgg
     1861 tcgcggtgac ccgccagatg cgcaccgcgc cgctgtgggg cgcccggttc aacacccagt
     1921 tcctgcacga cggtcgcgcc aagaccgtgc gcgccgcgat cctcgctcac gacggccagg
     1981 gtttcgcggc gcgcttcgcg tttgcggtgc tcgacccgat cagccaggcc ctgctgctcg
     2041 agttcgtcaa ctcgatctag gcctgcacga cagctcgacg gcggagggcc tcgcttcgtt
     2101 ctccagcgag cgccctccac cgcacatcca tgcacgctgg caccgaacgg cggaagcgcc
     2161 ggcaccgctt cagcgcgcca gggccttgaa cgcgaactcg cggccgcgct tgccgacggt
     2221 ggcgaggtgg atccaggatc gccagcgact cgaggtgcgc cgcgttgcgc agcgggccga
     2281 cgtcgacggg atcgtagccc agggtgcggg cgagctcggc gatgccgcgc cgggcgccgg
     2341 cgtcgtcgcc cgcgaggtag agatcggcgg cggcggaccc cagctcgggg cggtcgtgga
     2401 actcggcgcc gaacgtgttg aacgccttga tcaggcgcag cgcggggaac cgcttcgcca
     2461 ggtgggcggc catcgagccc tcgggcggcg gggtgtggac cgggccgtcg tcccagcgca
     2521 gcgggttggt gcagtcgacg acgatggtgc cggcggggag cccgcgcgcg gtgtcgagca
     2581 ccgccggtgc ggcggccgcg gggaccgcga ggaacagcgc gtcgaccgc
//
LOCUS       DASVOR010001817         4521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_164820,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001817 DASVOR010000000
VERSION     DASVOR010001817.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164820"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VF516_33770"
     CDS             <1..1271
                     /locus_tag="VF516_33770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112765.1"
                     /translation="MSRYSTDSHVFVQPFSRQPDGDEVVIGVQDSFIALPGAAVDLLD
                     WLATGKTVGEAQALYAEKHHEQPDMEDFLSSLEQVGFVQPQPADGAPPPTIAGAARKA
                     GTRQPVLRYHFENLPQPVARVLTSWGALGVYGAIIAAALAAIAAEPALWPRWNALLFT
                     ENMTPIGLALMLYSFWMLFLHEMAHLVAARAAGVPSRLGLGHRLWIVVAETDVSQLWT
                     LPRARRYMPLLAGPLFDLTAAAVFVCIGFAGSRGWLPLPPLAAQVCRALTLMHLLNLV
                     WQCNFFLRTDFYFVLANVFACKNLMGDTEAFLRTLAARWLRWGQAKDISYIPARELRV
                     VRGYAAIWILGRANMLWILVTITLPLMWGYLKQIATILGSGFSQHPFAFIDALLLGTI
                     TVTVQVTGLGMWLRSFLQQRKGGTPHEVAA"
     gene            1303..3147
                     /locus_tag="VF516_33775"
     CDS             1303..3147
                     /locus_tag="VF516_33775"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8112766.1"
                     /translation="MLAAPAGDVRQQHVTRWLDQAKQHGHSVWMLPCDRRLTGPWAGL
                     IELFGDLVPRLQSVAPRLLVQHDYELAIVLPTLQRMIEVRNPTLTDVSSETERTRNYP
                     LDRGYRIVHGLIDLLAEAQRVLGDPGYALACNDIDHAGALVRMFVSELVRRRGDRLGL
                     KLLVTCDPVSLETVTGQFQALDVGVRIDAAAAPPPAPAPHEMARRAEELERQAQKDHF
                     ERQFLLPQLIHTWLSSDQPARALPYQIEALSVYNTRGLYEDALFFGEPALEALERYTP
                     DALMDRMNICVKLYSSYLGMKRPHDAQRIAESAISHTDTPKLLGQWRYMTAMLNARFL
                     PQRNFDQAERDLEQGLVELEQSDLPHRTKAFLTSFNRNGLALVRYLQGRAEDAIALCQ
                     WGYAHLEAQLDGREQKLHRTVLVYNIAQVYSTMGNLEEAIRYLTEAMRDDPNYSEYYN
                     ERGSIYLKLERYQDAIVDYLRAIELSPPYMEVWTNLGQCYKAVGLLSQAVSAYSRALD
                     LDPRAALALIGRAQAHDGLGDRAAALADYDAVLALDPRQPLVLMNRGALHYENGALQQ
                     ALDDLDQAIALAPDMPELYENRAVALRALGRAELADGDHATAARLAVA"
     gene            3171..>4521
                     /locus_tag="VF516_33780"
     CDS             3171..>4521
                     /locus_tag="VF516_33780"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8112767.1"
                     /translation="MRLGDFARSRPVGVSSGLVMFLWTVAAGAAGDAPDQQPPLPAAL
                     ATTPIQVDGKLDEPAWQAAPVFDAFVQSYPKAGASPSERTELRILYDTDHLYVGVIAH
                     DSHPELIDRRLGRRDSSFLTDNVQLIIDSTHDHRTAHSFTLSAGGVQSDGLFYDDRNF
                     TSEWDAVWAGEAGALPDGWVAEFAIPLSALRFPEAPVQTWGFSVRRQIARLNEQIESV
                     DNPRASNVTVSRLGHLTGMKDLRPHIAVELLPYVATRGVLRPQFSDPARPHPRLFEPS
                     LDVGLDVKTAVTSSLVLNATLNPDFGQVEADRIIVNLTTYEVQFPEKRPFFTQGLELF
                     QPLGSDTQPVPQSLFYSRRIGLTTPILGAAKLTGNVSDRVTVGAFDALVASPWQPQDE
                     AAPDRRFQFQLARPLHLGPNSALPGSPLPTTDFLAAVVRASVGPSSRVGGYVAAALPL
                     "
BASE COUNT          700 a         1611 c         1498 g          712 t
ORIGIN      
        1 agatgagccg gtacagcacc gacagccacg tattcgtaca gccgttctcc cgacaacccg
       61 acggagacga ggttgtcatc ggcgtgcagg actccttcat tgcattgccc ggcgcggccg
      121 tggatctcct cgactggctc gccaccggaa agacggtggg cgaggcgcag gccctgtacg
      181 ccgagaagca tcacgagcag ccagacatgg aggacttcct cagctcgctc gagcaggtag
      241 ggttcgtgca gccgcaaccc gcggacggcg ctcccccgcc gacgatcgcg ggcgcggcgc
      301 gaaaggccgg caccaggcag ccggtcctgc gctaccactt cgagaacctc ccgcagccgg
      361 tggcgcgcgt cctgaccagc tggggcgccc tgggggtcta tggcgccatc atcgcggccg
      421 cgctggcggc gatcgcggcc gagccagcgc tgtggccgcg gtggaacgcg ctcctgttca
      481 ccgagaacat gacgccgatc gggctggcct tgatgctcta cagcttctgg atgctgttcc
      541 tccacgagat ggcgcacctc gtggcagcgc gggccgccgg cgtgccgtcg cgcctggggc
      601 tggggcaccg gctctggatc gtggtcgccg agaccgacgt gtcacagctg tggacgctgc
      661 cgcgagcgcg gcgctacatg cccctgctcg cgggaccgct gttcgacctc accgccgcgg
      721 cggtgttcgt gtgcatcgga ttcgccggct cacggggctg gctgccgctg ccgccgctgg
      781 cggcccaggt gtgccgcgcg ttgacgctga tgcacctgct caacctggta tggcagtgca
      841 acttcttcct ccgcaccgac ttctacttcg tgctggccaa cgtcttcgcc tgcaagaacc
      901 tgatgggaga caccgaggcg ttcctgcgca cgctggccgc caggtggctg cgctgggggc
      961 aggccaagga catctcctac atcccggccc gcgagctgcg ggtcgtgcgt ggctacgccg
     1021 cgatctggat cctgggccgc gcgaacatgc tgtggatcct cgtcaccatc acgctgcccc
     1081 tgatgtgggg atacctgaaa cagattgcaa cgatcctcgg ttcgggcttt tcacaacatc
     1141 cattcgcttt catcgacgcg ctactgctcg ggaccatcac cgtgaccgtc caggtgaccg
     1201 gcctggggat gtggttgcgc agctttctcc aacagagaaa aggaggcacc ccgcatgagg
     1261 tggcagcctg agttgtcgtt tgacgcatct gatcgtttca ccttgctggc ggcccccgct
     1321 ggagacgtcc gccagcagca cgtcacgcgc tggctggacc aggcgaagca gcacgggcat
     1381 tcggtctgga tgctgccctg cgatcgccgg ctcacggggc catgggccgg cctgatcgag
     1441 ctgttcggtg atctggtccc gaggctccag tccgtggcgc cgcggctgct ggttcagcat
     1501 gactacgagc tggcgatcgt gctgccgacc ttgcagcgca tgatcgaggt ccggaatccg
     1561 accctgacgg acgtcagctc ggagaccgag cgcacgcgca actacccgct ggatcgcggt
     1621 taccgcatcg tccacggcct gatcgatctc ctggcggagg cccagcgagt cctgggagat
     1681 ccaggctacg cgctggcctg caacgacatc gaccacgcgg gggcgctcgt gcggatgttc
     1741 gtctcggagc tggtgcgccg caggggcgat cggctcggcc tgaagctgct ggtcacctgc
     1801 gatccggtgt cgctcgagac cgtcaccggc cagttccagg ccctggacgt cggggtgcgg
     1861 atcgacgcgg ccgccgcgcc gcctcccgcc ccggcgccgc acgagatggc ccggcgagca
     1921 gaggagctgg agcgccaggc ccagaaagac cacttcgagc gccagttcct gctgccccag
     1981 ctgatccaca cctggctgag cagcgatcag cccgccaggg cgctgccgta ccagatcgaa
     2041 gcgctgtcgg tctacaacac gcgcggcttg tacgaggacg cgctgttctt cggcgagccg
     2101 gcgctcgagg cgctggagcg gtacacgccg gacgcgctca tggatcgcat gaacatctgc
     2161 gtcaagctgt acagcagcta tctcggcatg aagcgaccgc acgatgccca gcgcatcgcc
     2221 gagtccgcga tctcgcacac cgacaccccg aagctgctcg ggcagtggcg ctacatgacc
     2281 gccatgctca acgcccggtt cctcccgcag cgcaacttcg atcaggcaga gcgcgacctc
     2341 gagcaaggcc tggtggagct cgagcaatcc gatctcccgc accgcaccaa ggccttcctg
     2401 acctcgttca accgcaacgg gctggccctc gtacgctatc tccagggccg cgccgaggac
     2461 gcgatcgccc tgtgccagtg gggctacgcg cacctcgaag cccagctcga cggccgagag
     2521 cagaagctcc accgcacggt gctggtctac aacatcgccc aggtctacag caccatgggc
     2581 aacctcgagg aggcgatccg ctacctcacc gaggccatgc gcgacgaccc caactactcg
     2641 gagtactaca acgagcgcgg cagcatctac ctgaagctcg agcgctacca ggacgcgatc
     2701 gtcgattatc tgcgcgcgat cgagctgagc ccgccctaca tggaggtgtg gaccaacctc
     2761 ggtcagtgct acaaggccgt cggcctgctg tcccaggcgg tctcggccta ttcgcgcgcg
     2821 ctcgatctcg atcccagggc ggcgctggcc ctgatcggcc gtgcccaggc gcatgatggt
     2881 ctgggcgatc gcgcggccgc cctggcggac tacgacgcgg tgctggcgct ggatccccgc
     2941 cagccgctgg tgctgatgaa ccgcggcgcg ctgcactacg agaacggcgc tctgcagcag
     3001 gcgctcgacg atctcgacca ggcgatcgcg ctcgccccgg acatgccgga gctgtacgag
     3061 aaccgggcgg tcgcgctgcg cgcgctgggc cgcgcggagc tcgccgatgg cgaccacgcc
     3121 acggccgcac gccttgccgt cgcgtgagcg gcgagcgaac atgcccgcag atgaggctcg
     3181 gagacttcgc gcggagccgc cctgtgggtg tgtcgtcggg gctggtcatg tttctgtgga
     3241 ccgtggcggc cggcgcggcg ggcgacgccc ccgaccagca gccgccgctc ccggcagcgc
     3301 tggccaccac gccgatccag gtcgacggaa agctcgacga gccggcgtgg caagccgcac
     3361 ccgtgttcga cgcgttcgtg cagagctacc ccaaggccgg cgcgtccccg agcgagcgga
     3421 ccgagctgcg catcctctac gacacggatc acctgtacgt cggtgtcatc gcccacgatt
     3481 cgcaccccga gctcatcgac cgccggctgg gccgccgcga tagctcgttc ctcaccgaca
     3541 acgtacagct gatcatcgac tccacgcacg accaccgcac cgcccattcg ttcacgctga
     3601 gcgccggcgg cgtccagagc gacgggctgt tctatgacga tcgcaacttc acgtccgagt
     3661 gggacgcggt gtgggccgga gaggccggcg cgctgcccga cggctgggtg gcggagttcg
     3721 cgattccgct gtcggcgctg cgcttcccgg aggcgccggt gcagacctgg gggttctcgg
     3781 tgcgccgcca gatcgcccgg ctcaacgagc agatcgagtc ggtggacaac ccgcgcgcga
     3841 gcaacgtgac cgtgtcgcgg ctgggccacc tcaccggcat gaaggacctc cggccgcaca
     3901 tcgccgtgga gctgcttcct tatgtcgcga cccgcggcgt gctccggccg cagttctcgg
     3961 atcccgcgcg cccccacccc cgcctgttcg agccgagcct cgacgtcggg ctggacgtca
     4021 agacggcggt gaccagcagt ctggtgctca atgccacctt gaaccccgac ttcggacagg
     4081 tcgaggcgga tcggatcatc gtcaatctga ccacgtacga ggtccagttc ccggagaagc
     4141 gcccgttctt cacccaggga ctcgagctgt tccagccgct gggcagcgat acccagccgg
     4201 tgccgcagag cctgttctac tcgcggcgca tcgggctcac cacgccgatc ctgggcgcgg
     4261 cgaagctgac cggcaacgtg tcggatcgcg tgacggtggg agcgttcgac gccctcgtcg
     4321 cgagcccgtg gcagccgcag gacgaggccg cccccgaccg gcggttccag ttccagctcg
     4381 cccggccgct gcacctcggc cccaacagcg cgctgccggg cagcccgctg ccgaccaccg
     4441 acttcctggc cgcggtcgtg cgcgccagcg tcgggcccag ctcgcgcgtc ggcgggtacg
     4501 tcgcggcggc cctgccgctg g
//
LOCUS       DASVOR010001818         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_427430,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001818 DASVOR010000000
VERSION     DASVOR010001818.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427430"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VF516_33785"
     CDS             complement(<1..1199)
                     /locus_tag="VF516_33785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEX8112768.1"
                     /translation="MAKFQWEATTRTGEKKRGVMEAEAQAQVEARLRGDGLTIDRVRK
                     EPVQIYITFGSGVGAKDLQIFTRQLATMIDAGLPLVQCLDILAAQTPNKIFARILGQV
                     KSAVEQGSTFSDALRRHPRVFDDLYVNLVAAGEVGGILDTILNRLAVYIEKAVKLRGQ
                     IKSAMFYPTGILVVAVGVIAVMLLKVIPTFESMYREMNHAQLPAATQFVISLSHGFIN
                     RWYLYFGTLFGVIAGSTAMRRTDRGKEIFDRMLLRLPVMGPVLRKIVVARFTRTLGTL
                     LTSGVPILDALDICGRTAGNKVVEHGIQKARDKIAEGHDMAGPLAESRVFPTMVVQMI
                     GVGEQTGAMDQMLQKIADFYEEEVDAAVSALTSLIEPVMMAFLGVVVGGLIIAMYLPI
                     FKLAGNIG"
     gene            complement(1217..>1592)
                     /locus_tag="VF516_33790"
     CDS             complement(1217..>1592)
                     /locus_tag="VF516_33790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HEX8112769.1"
                     /translation="SVNLVLAQRLARRICADCKIPDEKHPEALAKMGMTEDQIRKVTI
                     MKGRGCNTCNNTGYKGRVALYEVMPFTDPLKELVLQGASATEIKTEMIRGGVQSLRMA
                     GLAKIMEGVTTPEEVLRTTVED"
BASE COUNT          240 a          537 c          533 g          282 t
ORIGIN      
        1 ccgatgttgc cggcgagctt gaagatcggc aggtacatcg cgatgatcag gccgccgacc
       61 acgacgccga ggaacgccat catcaccggc tcgatcagcg aggtcagcgc cgacaccgcg
      121 gcgtcgacct cctcctcgta gaagtcggcg atcttctgca gcatctggtc catcgcgccg
      181 gtctgctcgc cgacgccgat catctggacg accatggtcg ggaacacccg ggactcggcg
      241 agcggccccg ccatgtcgtg gccctcggcg atcttgtcgc gcgccttctg gatgccgtgc
      301 tcgaccacct tgttgcccgc ggtgcggccg cagatgtcga gcgcgtcgag gatcggcacg
      361 ccggaggtca gcagcgtgcc gagcgtccgg gtgaaccgcg cgacgacgat cttgcgcagc
      421 accggcccca tcaccggcag ccggagcagc atgcggtcga agatctcctt gccgcgatcg
      481 gtccgccgca tcgcggtcga gccggcgatc acaccgaaca gcgtgccgaa gtacaggtac
      541 cagcggttga tgaagccgtg cgacaggctg atcacgaact gggtcgcggc cggcagctgc
      601 gcgtggttca tctcgcggta catgctctcg aacgtcggga tgaccttgag cagcatcacc
      661 gcgatcacgc cgaccgcgac gaccaggatg ccggtcgggt agaacatcgc gctcttgatc
      721 tggccgcgca gcttgaccgc cttctcgatg tagaccgcga gccggttgag gatcgtgtcg
      781 aggatgccgc cgacctcgcc ggccgcgacc aggttgacgt acaggtcgtc gaacaccctg
      841 gggtgccggc gcagcgcgtc ggagaacgtc gagccctgct cgaccgcgct cttcacctgg
      901 cccaggatgc gcgcgaagat cttgttgggg gtctgcgcgg cgaggatgtc gaggcactgg
      961 accagcggca gaccggcgtc gatcatcgtc gcgagctggc gggtgaagat ctgcaggtcc
     1021 ttggcgccga cgccggagcc gaacgtgatg tagatctgga ccggctcctt gcggacgcgg
     1081 tcgatcgtca ggccatcgcc gcgcagccgg gcctcgacct gggcctgggc ctcggcctcc
     1141 atgacgccgc gcttcttctc accggtgcgc gtggtcgctt cccactggaa cttggccatg
     1201 gcgttctcct agctagctag tcctccaccg tggtgcggag gacctcttcc ggcgtggtca
     1261 ccccctccat gatcttggcg agcccggcca tgcgcaggct ctgcacgccg ccgcggatca
     1321 tctcggtctt gatctcggtg gccgacgcgc cctgcagcac gagctccttg agcggatcgg
     1381 tgaacggcat cacctcgtag agcgcgaccc ggcccttgta gccggtgttg ttgcaggtgt
     1441 tgcagccgcg gcccttcatg atcgtgacct tgcggatctg gtcctcggtc atccccatct
     1501 tggcgagcgc ctcggggtgc ttctcgtcgg ggatcttgca gtcggcgcag atcctgcggg
     1561 ccagccgctg ggcgagcacg aggttgaccg ag
//
LOCUS       DASVOR010001819         2537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_164887,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001819 DASVOR010000000
VERSION     DASVOR010001819.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2537
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164887"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1660
                     /locus_tag="VF516_33795"
     CDS             110..1660
                     /locus_tag="VF516_33795"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX8112770.1"
                     /translation="MTAVADRPADPPALLRMEGISKHYGGVRALDGAALRCARGRIHA
                     VLGENGAGKSTLIKVLSGVVRPDAGTIELDGAPVELRRPADAHRLGIVSVFQELSLLP
                     DLTVADNIGIVDPPRRWGLIDARAQRRRAEAALARLGVTDIHPLAPVRDLPLSRRQIV
                     EIAKALARDPKLLILDEATSALTAADVASVFGVLRALRDQGLAIVFISHRMHEIDELA
                     DDCSVYRNGRHVASFAAGERTANQIIEMMIGRDYDSVFPPRPRRTANEPPALQVNGLS
                     WAHRLRDIRFAIRPGEVVGLGGLDGQGQRDLLLALFGALTGVTGEARVHGERIALSSP
                     RTTRHGGIALIPEDRKTEGLMLAMSVRDNLSFAALDQLSRFGVIDRRAEHAAVAGMVD
                     LLRIRSDALDGPVAALSGGNQQKVVIGKWLLTRPQIILLSDPTRGIDVGTKQELYQLL
                     RRLADERAAILLHSTDYAELIGCCDRVLILYDGAIVRELEGDAITEHNIVAAAHNLPA
                     GSPVEPRA"
     gene            1657..>2537
                     /locus_tag="VF516_33800"
     CDS             1657..>2537
                     /locus_tag="VF516_33800"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8112771.1"
                     /translation="MTGRRTDLGYWLAAQRGPLAAAALFVAMFALFVAKHPAGPSGNV
                     INTAANKGALLALVAMAQTLPVLTSGLDLSVGSVFVMTNCLASTVIAGTPGQVALGVA
                     LVLASGLVCGAINGLVVVVGRLQPIIATLATGAVYQGIALWLRPAPGGNVHRGFADFM
                     TSSLHDAVPVSLLVLLATVVVIWLPYRRSALGRAAYAIGSSEQAAYMSGVPVARAKLA
                     TYVLAGLLAAIAGLLLTCVTESGEANALLAGSYTLYSIAAVVIGGTALYGGSGGAIGT
                     IFGAFVLRTIGDLLFVF"
BASE COUNT          320 a          960 c          881 g          376 t
ORIGIN      
        1 tatcccgcgc tgtcggacaa cttcttcgtc ggcaactcgt tcccggactg cagcatcggg
       61 ttcacggccg acgagatcat gggcaagtcg gaggccaacc agtagcgccg tgaccgccgt
      121 cgccgatcgg ccggccgacc cgcctgcgct cctccgcatg gaggggatct cgaagcacta
      181 cggcggcgtg cgcgcgctcg acggtgccgc cctgcgatgc gcccgcggtc ggatccacgc
      241 cgtgctcggc gagaacggcg ccggcaagtc gacgctgatc aaggtgctgt cgggcgtggt
      301 ccggcccgac gcggggacga tcgagctcga cggtgcgccg gtcgagctgc gccggccggc
      361 cgacgcgcac cggctcggga tcgtgagcgt gttccaggag ctgtcgctct tgcccgacct
      421 cacggtcgcc gacaacatcg gcatcgtcga cccgccgcgg cgctgggggc tgatcgacgc
      481 gcgcgcccag cgccggcgcg ccgaggcggc cctggcgcgg ctcggggtca ccgacatcca
      541 cccgctcgcg ccggtccgcg acctgccgct gtcgcgccgc cagatcgtcg agatcgccaa
      601 ggcgctggcc cgcgatccga agctgctgat cctcgacgag gcgacctcgg cgctgaccgc
      661 ggccgacgtc gcatcggtgt tcggcgtgct gcgcgcgctg cgcgaccagg ggctcgcgat
      721 cgtgttcatc tcgcaccgca tgcacgagat cgacgagctc gccgacgact gctcggtgta
      781 tcgcaacggc cgccacgtcg cgagcttcgc cgccggcgag cgcacggcga accagatcat
      841 cgagatgatg atcggccgcg actacgacag cgtgttcccg ccccgccccc gccgcacggc
      901 gaacgagccg cccgcgctgc aggtgaacgg gctgtcgtgg gcccaccggc tgcgcgacat
      961 ccgcttcgcg atccgccccg gcgaggtggt cgggctcggc gggctcgacg gccagggcca
     1021 gcgcgacctc ttgctcgcgc tgttcggcgc gctgaccggc gtcaccggcg aggcccgggt
     1081 ccacggcgag cggatcgcgc tgtcgagccc gcgaaccacc cggcacggcg ggatcgcgct
     1141 gatccccgag gaccgcaaga ccgaggggct gatgctggcg atgtcggtgc gcgacaacct
     1201 gtcgttcgcg gcgctcgatc agctctcccg gttcggcgtg atcgatcgcc gggccgagca
     1261 cgccgcggtc gccggcatgg tcgacctgct gcggatccgc agcgacgcgc tcgacggccc
     1321 ggtcgccgcg ctgtcgggcg gcaaccagca gaaggtggtg atcggcaagt ggctcttgac
     1381 ccggccgcag atcatcctgc tcagcgatcc gacccgcggc atcgacgtcg gcaccaagca
     1441 ggagctctac cagctgctgc gccggctcgc cgacgagcgc gccgcgatcc tgttgcactc
     1501 gaccgactac gccgagctga tcggctgctg cgaccgcgtg ctgatcctct acgacggcgc
     1561 gatcgtccgc gagctcgagg gcgacgcgat caccgagcac aacatcgtcg ccgccgccca
     1621 caacctgccg gccggctcgc cggtcgagcc gcgcgcatga ccggccggcg caccgatctc
     1681 ggatactggc tcgccgcgca gcgcggcccg ctcgccgccg ccgcgctgtt cgtcgcgatg
     1741 ttcgcgctgt tcgtcgccaa gcatccggcc ggcccgagcg gcaacgtgat caacaccgcc
     1801 gccaacaagg gcgcgctgct cgcgctggtc gccatggccc agaccctccc cgtgctgacc
     1861 tcggggctcg atctgtcggt cggcagcgtg ttcgtgatga ccaactgcct ggcgtcgacc
     1921 gtgatcgccg ggacgccggg ccaggtcgcg ctcggcgtcg ccctcgtgct ggcgagcggc
     1981 ctcgtgtgcg gcgcgatcaa cggcctcgtc gtcgtggtcg gccggctgca gccgatcatc
     2041 gcgaccctgg cgaccggcgc ggtgtaccag ggcatcgcgc tctggctgcg cccggccccc
     2101 ggcggcaacg tccaccgggg cttcgccgac ttcatgacca gctcgctgca cgacgccgtc
     2161 ccggtgtcgc tgctcgtgct gctcgccacc gtggtcgtga tctggctgcc gtatcgccgc
     2221 tccgcgctcg gccgcgcggc ctacgcgatc ggctcgtcgg agcaggccgc gtacatgtcc
     2281 ggcgtgccgg tcgcccgcgc caagctcgcg acctacgtgc tcgccggcct gctcgccgcg
     2341 atcgccggcc tcttgctcac ctgcgtcacc gagtccggcg aggccaacgc gctgctcgcc
     2401 ggcagctaca cgctgtactc gatcgcggcg gtcgtgatcg gcggcaccgc gctgtacggc
     2461 ggatcgggcg gcgcgatcgg cacgatcttc ggcgcgttcg tgctccgcac gatcggcgac
     2521 ctgctgttcg tgttcga
//
LOCUS       DASVOR010001820         3291 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_545260,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001820 DASVOR010000000
VERSION     DASVOR010001820.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545260"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VF516_33805"
     CDS             <1..189
                     /locus_tag="VF516_33805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112772.1"
                     /translation="DQHDLALRALGKATDTGLIEVTWLRGCPLFTPITGDLRWRAVHD
                     AVERRAAAMLATFRAAAG"
     gene            370..1764
                     /locus_tag="VF516_33810"
     CDS             370..1764
                     /locus_tag="VF516_33810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112773.1"
                     /translation="MPVTIPWRTALAVAALTVGCGTKHTVDRDPGNIDLPPDDPSQSS
                     PPDPGAPPPDTTPPGHPPPGNPPPAPACAIGGVTGNARFSIDVGHGAFDLAGDVLRSD
                     LAVDAAGNTFLALGTATGFTLTKYAPDGKVVFQKPFGEVVAVDAAGNIYVAATFSAAL
                     DLGFGTMMPSGDMDVFVAKLAPDGTLLFARPLGVCPDSLRSIAVACDGRIAVSGAEMG
                     TLVLDAKGALLFSLPFDGDLAFDSKGNLIVGGQFTGTLDLGGGARMTAGDIDGFVVKL
                     DVSGSVLWSYQIGDADLPVMLKPSSMMVSTPTRQGVSAVAVGPDDEVVIAGQFDDDAK
                     LFGSDFVANLVDAASVPLQSGGFVVALDAAGAVKLRSVRLGLDGYRDVAVAANGDIVV
                     TGKLLGEAAPPFRFALLTRLTASGAARFEQSNDIGAGHAVAVDACGAPVWLMSARPLG
                     VAELPEAFLNKLVP"
     gene            complement(1769..2014)
                     /locus_tag="VF516_33815"
     CDS             complement(1769..2014)
                     /locus_tag="VF516_33815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112774.1"
                     /translation="MAVRYKFVELSTVTDETLEHAVNHWVAQGWQLDGIRFVVTEHSR
                     RPAMAFVSFTCEAVGGGAGDAPDTPAHVIAADDNDVE"
     gene            complement(2017..2997)
                     /gene="hemB"
                     /locus_tag="VF516_33820"
     CDS             complement(2017..2997)
                     /gene="hemB"
                     /locus_tag="VF516_33820"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEX8112775.1"
                     /translation="MNYPDYRPRRMRRTEGLRRLVRETRLSVDDFVYPLFCVPGTGVD
                     KPIPSMPGQSNLSVDKATEAAARAHDLGIPAVLLFGIPEHKDAVGSEAWKDTGVVQKA
                     IRSIKKQCPDMVVMVDACFCEYTDHGHCGVLRDKELDNDATLENLARTVVSYARCGAD
                     VVAPSGMLDGFVGACRESLDEEHFDQVAIMAYSAKYASGYYGPFREAVDSAPQHGDRR
                     GYQMDPANVAEAVREVSMDVAEGADIVMVKPALAYLDVIRAVSDEINMPIAAYNVSGE
                     YAMIEAAAANGWIDKERIVMETLLSMRRAGSDIIITYHAPYAAKLLQQRR"
     gene            complement(2994..>3291)
                     /locus_tag="VF516_33825"
     CDS             complement(2994..>3291)
                     /locus_tag="VF516_33825"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEX8112776.1"
                     /translation="AVVAYRTVPATADDPALAAGLAALRDGAAGVCVVFAPSQVAALD
                     ALIGIRRISAPFAAIGETTAQALRAAGAGVVTVAEAPTPEGLAKAVSAVYPRRS"
BASE COUNT          444 a         1223 c         1138 g          486 t
ORIGIN      
        1 gatcagcacg acctcgcgct gcgcgcgctc ggcaaggcga ccgataccgg cctgatcgag
       61 gtgacgtggc tgcgcggttg cccgctgttc acgccgatca ccggcgatct gcggtggcgg
      121 gcggtccacg acgcggtcga gcggcgcgcc gcggcgatgc tcgcgacatt ccgcgccgcc
      181 gctgggtagc gcctcacctc aggagatccc cgagggcggg tgcccaggag cgttcgcgcc
      241 gcggcgagcg cgtcacagcg acgagcccgc gcacggatcg cggttcttcg cacgagcact
      301 tgcgccgcgc cgggcgtggc acgagccatg aactgcgggc tgaaaaacag aaagccttgg
      361 agagccccaa tgcccgtcac catcccctgg cggaccgcgc tcgcggtcgc cgccctcacc
      421 gttggctgcg gtaccaagca caccgtggat cgcgatcccg gcaacatcga tctgccgccg
      481 gacgacccgt cgcagtcgtc cccgcccgat cccggcgccc cgccgcccga caccacgccg
      541 cccggccatc cgccgcccgg caatccgccc cccgcgccgg cgtgcgcgat cggcggggtc
      601 accggcaacg cgcggttctc gatcgacgtc ggccacggcg cgttcgacct cgcgggtgac
      661 gtgctgcgct ccgatctcgc ggtcgacgcc gccggcaaca cgttcctcgc gctgggcacc
      721 gccaccgggt tcacgctgac caagtacgcg cccgacggca aggtggtgtt ccagaagccg
      781 ttcggcgagg tcgtcgcggt cgacgccgcc ggcaacatct atgtcgcggc gacgttcagc
      841 gcggcgctcg acctcggttt cggcacgatg atgccgtccg gcgacatgga cgtgttcgtc
      901 gccaagctcg cgcccgatgg cacgctgctg ttcgcgcgcc cgctcggggt gtgtccggac
      961 agcctgcgca gcatcgcggt ggcctgcgac ggccggatcg cagtgtcggg cgccgagatg
     1021 ggcacgctcg tgctcgacgc caagggcgcg ctgctgttca gcctgccgtt cgacggcgat
     1081 ctcgcgttcg actcgaaggg caacctgatc gtcggcggcc agttcaccgg gacgctggac
     1141 ctcggcggcg gcgctcgcat gaccgccggc gacatcgacg gcttcgtggt caagctcgac
     1201 gtcagcggca gcgtgctgtg gagctaccag atcggcgacg ccgacctgcc ggtcatgctg
     1261 aagccgagca gcatgatggt atcgaccccg acccgccagg gggtgtccgc ggtcgcggtc
     1321 ggccccgacg acgaggtcgt gatcgccggc cagttcgacg acgacgccaa gctgttcggc
     1381 agcgacttcg tcgccaacct ggtcgacgcc gcgtcggtgc cactgcagtc gggcggcttc
     1441 gtggtcgcgc tcgacgcggc cggcgccgtg aagctccgct cggtgcggct cgggctcgac
     1501 ggctaccgcg acgtcgcggt ggccgcgaac ggcgacatcg tcgtgaccgg caagctgctc
     1561 ggcgaggccg cgccgccgtt ccggttcgcg ctgctgaccc ggctgaccgc gtccggcgcg
     1621 gcccggttcg agcagagcaa cgacatcggc gcgggccacg cggtcgcggt cgacgcctgc
     1681 ggcgccccgg tctggctgat gtcggcccgc ccgctcggcg tcgccgagct gcccgaggcg
     1741 ttcctcaaca agctcgtacc gtgaccggct actcgacgtc gttgtcgtcg gcggcgatca
     1801 cgtgcgccgg cgtgtccggt gcgtcgcccg cgccgccgcc caccgcctcg caggtgaacg
     1861 acacgaacgc catcgccggg cgccggctgt gctcggtgac cacgaaccgg atgccgtcga
     1921 gctgccagcc ctgcgcgacc cagtggttga ccgcgtgctc gagcgtctcg tcggtgacgg
     1981 tcgacagctc gacgaacttg tagcgcaccg ccacggctac cggcgctgct gcagcagctt
     2041 ggcggcgtac ggcgcgtggt aggtgatgat gatgtcgctg ccggcccgcc gcatcgacag
     2101 cagcgtctcc atcacgatcc gctccttgtc gatccagccg ttggccgccg cggcctcgat
     2161 catcgcgtac tcgccgctga cgttgtaggc cgcgatcggc atgttgatct cgtcgctgac
     2221 cgcgcggatc acgtcgaggt acgccagcgc cggcttgacc atcacgatgt cggcgccctc
     2281 ggcgacgtcc atcgacacct cgcgcaccgc ctcggcgacg ttggccgggt ccatctggta
     2341 gccacggcgg tcgccgtgct gcggcgccga gtcgaccgcc tcgcggaacg gcccgtagta
     2401 gcccgacgcg tacttggccg agtacgccat gatcgcgacc tgatcgaagt gctcctcgtc
     2461 caggctctcc cggcacgcgc cgacgaagcc gtcgagcatg ccgctcggtg cgaccacgtc
     2521 ggcgccgcac cgggcgtacg acaccacggt gcgcgccagg ttctccagcg tcgcgtcgtt
     2581 gtcgagctcc ttgtcgcgca gcacgccgca gtggccgtga tcggtgtact cgcagaagca
     2641 ggcgtcgacc atcacgacca tgtccggaca ctgcttcttg atcgagcgga tcgccttctg
     2701 gaccaccccg gtgtccttcc aggcctcgct gccgaccgcg tccttgtgct cggggatgcc
     2761 gaacagcagc accgccggga tgccgaggtc gtgcgcgcgc gccgcggcct cggtcgcctt
     2821 gtcgaccgac aggttggact ggcccggcat cgacgggatc ggcttgtcga ccccggtgcc
     2881 cggcacgcag aacagcgggt agacgaagtc gtcgacggac aggcgcgtct cgcggaccag
     2941 gcggcgcagc ccctcggtcc ggcgcatccg gcgcgggcgg taatccgggt agttcatgac
     3001 ctacggggat acaccgccga caccgccttg gccaagcctt ccggtgtggg cgcctccgcg
     3061 acggtcacca cgccggcccc ggccgcgcgc aatgcctgcg cggtggtctc accgatcgcc
     3121 gcgaacggcg ccgagatccg gcggatgccg atcagcgcgt cgagcgcggc gacctgcgag
     3181 ggtgcgaaca ccacgcacac cccggccgca ccgtcgcgca gcgcagcgag ccctgcggcg
     3241 agcgccggat cgtcggcggt cgccggcacc gtgcggtacg cgaccacggc c
//
LOCUS       DASVOR010001821         5817 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_20214,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001821 DASVOR010000000
VERSION     DASVOR010001821.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5817
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20214"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1068)
                     /locus_tag="VF516_33830"
     CDS             complement(<1..1068)
                     /locus_tag="VF516_33830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112777.1"
                     /translation="MSAVATAAGLPTHLGKYEILTPLASGGMARIYVARVTGIGAFER
                     HVVLKVISPERAQDVTSVQMFLDEARLAASLNHHNVAQVFEVGEEAGIHFLAMEYVHG
                     QDLRALLAKAGSTATRVPLELALTVVAGAAAGLDHAHERHSPDGTPMGIVHRDVSPSN
                     IMIGYDGAVKLLDFGIAKAAARSVETQSGIIKGKFAYMAPEQCRGRDVDRRSDVFSLG
                     IILYEVSTQHRCFRADSDFDTMHRIVTGDIVRPTRLIHGYPPALEAIVMKALAIDPGQ
                     RYASAGQLLEALEGFAAVARLSLSTVALGRFLRDMFGDVPEPWLQTGDFQRVAPPTEH
                     TISSTSAPGEAPHGQPPVPRSA"
     gene            complement(1158..2579)
                     /locus_tag="VF516_33835"
     CDS             complement(1158..2579)
                     /locus_tag="VF516_33835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEX8112778.1"
                     /translation="MTAARSMSGAAGVTDRTVQELREIAQLAVELARRAGADDAEALV
                     REGNELTVKVRLGEPELVQEAGSRALGLRVLRGGRRAVTYTSDLRREALEALCAESVT
                     LADLAEVDEYALPPDPALLAKDVPDLDLYDPRVAEVDAAWALRQAIAGESAARKHDPR
                     VTNSEGAIWSRVLGATGFATSGGFVGGYRGSYASLFVEPLADDTTDPTNPKKRNGYWW
                     SGSRFLGELEPAEAVGIEAARRTVATIGSRKVETQECPIVFDPEMARSIIGMLFSVAN
                     GASFWRKSSYLIGKEGQAIASPLVTVVDDPLIPRAPGSRPFDGDGLATRRNLVVERGV
                     LCPVLCDVYSARKLGRQSTGSSGRTIGSSPGPTTSNLIMQAGAMSRQELLRNTHKGLY
                     VTHMMGFGFNAVTGDFSRGAQGFWIENGELTYPVSEVTIAANFDDILKRIDAVADDLQ
                     MRSATAAPTFRVSQMTLAGTSKN"
     gene            complement(2576..4036)
                     /locus_tag="VF516_33840"
     CDS             complement(2576..4036)
                     /locus_tag="VF516_33840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX8112779.1"
                     /translation="MITSLPAALRAPFSPGGGNAIDAQLAHRLLSIALEAGGDYADLY
                     FEFRVSADYALEEEQIRALGRGITLGLGVRVTKGDATGYAYCEDLAWDRMAHAARTAG
                     QIAVGGGHSPVGVAPISTVAVPSFYGVPMPSLMVSAHDKLALLRAGDRAARAYDPRIV
                     KVEAFLSEVIKEMLVFTSDGRMASDLQPLIRYGVRAVAERNDKRQSGSGGGGGRYGME
                     YFQLAERDAESHGREAARVAIAMLDAREVPAGEMPVVLAAGDSGILLHEAVGHGLEAD
                     FNRKQTSNYSGQMGKLVASTLCTVVDDGTIGNSRGAINVDDEGYPGQSNTLIENGVLV
                     GYMHDRLSAKHFGGAPTGNGRRESYRAMPLPRMTNTALLGGKDNPEDIVKSVKRGIFA
                     KGFSGGSVNITNGDFVFSLSESYLIEDGKLTAPLKGAQLIGNGPDVLRRVDMVGSDFQ
                     LSDGVWSCGKDGQSVPVNVGTPTIRIASITVGGTQA"
     gene            complement(4400..5020)
                     /locus_tag="VF516_33845"
     CDS             complement(4400..5020)
                     /locus_tag="VF516_33845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HEX8112780.1"
                     /translation="MELRALTVLDSLQPQLTGFLQTVCSGFMPLEEEAALFLEIAPGI
                     AINQLTDAALKATRCRPGMQIVERAYGMLELHHGDKGQVEAAADAIVARMAVQREDRI
                     KPRIVSSQILTGIDGKQSQLINRMRHGDMILSGQTLYILEVHPAAYAALAANEAEKAA
                     NIHLLEVITFGAFGRLWLGGTEAEIQAAVEAAEGALAKLSGRDARG"
BASE COUNT          835 a         2061 c         2033 g          888 t
ORIGIN      
        1 cgccgagcgc ggcaccggcg gctgcccgtg cggcgcctcg ccgggcgcgc tggtgctgga
       61 gatcgtgtgc tccgtcggcg gcgcgacccg ctggaagtcg ccggtctgca gccacggctc
      121 ggggacgtcg ccgaacatgt cgcgcaggaa ccggccgagc gccacggtcg acagcgacag
      181 ccgcgccacc gccgcgaagc cctcgagcgc ctcgagcagc tggccggccg acgcgtagcg
      241 ctggcccgga tcgatcgcga gcgccttcat gacgatcgcc tcgagcgccg gcggatagcc
      301 gtggatcagc cgggtcggcc gcacgatgtc gccggtgacg atccggtgca tcgtgtcgaa
      361 gtcgctgtcg gcgcgaaagc agcggtgctg ggtcgagacc tcgtacagga tgatgccgag
      421 cgagaacacg tcgctgcgcc ggtcgacgtc gcggccgcgg cactgctcgg gcgccatgta
      481 cgcgaacttg cccttgatga tgccgctctg ggtctcgacc gagcgcgccg ccgccttggc
      541 gatgccgaag tcgagcagct tcaccgcgcc gtcgtagccg atcatgatgt tcgacggcga
      601 cacgtcgcgg tggacgatgc ccatcggcgt gccgtcgggg ctgtggcgct cgtgcgcgtg
      661 atcgagcccg gcggccgccc cggcgacgac ggtgagcgcg agctcgagcg gcacccgcgt
      721 cgccgtgctc ccggccttgg cgagcagcgc gcgcaggtcc tggccgtgca cgtactccat
      781 cgccaggaag tggatgccgg cctcctcgcc gacctcgaac acctgcgcga cgttgtggtg
      841 gttcaaggac gcggcgagcc gggcctcgtc gaggaacatc tgcaccgagg tcacatcctg
      901 tgcgcgctcg ggcgagatca ccttcagcac gacgtggcgc tcgaacgcgc cgatgccggt
      961 cacgcgcgcc acgtagatcc gcgccattcc tccggacgcc agcggcgtga ggatctcgta
     1021 cttgccgagg tgggtgggta gcccggctgc cgtggctacg gccgacaccg acagagcgtt
     1081 tcaccccgcg tcatgcgagt caaccaaaac gctccccggg gctgcccgga gctgccaagg
     1141 aaatcgagca tgtaggttca gttcttcgag gtccctgcga gtgtcatctg cgatacgcga
     1201 aacgtcgggg ccgcggtcgc gctgcgcatc tgcaggtcgt cggcgacggc gtcgatccgc
     1261 ttcaggatgt cgtcgaaatt cgcggcgatg gtgacctcgc tcaccgggta cgtgagctcg
     1321 ccgttctcga tccagaagcc ctgggcgccg cgcgagaaat ccccggtgac ggcgttgaag
     1381 ccaaagccca tcatgtgcgt gacgtagagt cccttgtggg tattgcgtaa caactcctga
     1441 cgcgacatgg ctccggcttg catgatcagg ttcgaagtgg tcgggccagg gctgctgccg
     1501 atggtgcgcc ccgacgagcc ggtcgactgc cggccgagct tgcgggcgct gtagacgtcg
     1561 cacaggaccg ggcacagcac gccgcgctcg accacgaggt tgcggcgggt cgccaggccg
     1621 tcgccgtcga acggccgcga gcccggcgcg cgcgggatca gcgggtcgtc gaccacggtg
     1681 accagcggcg aggcgatggc ctggccttcc ttgccgatca ggtagctcga cttgcgccag
     1741 aacgacgcgc cgttggcgac cgagaacagc atgccgatga tcgagcgcgc catctcgggg
     1801 tcgaacacga tcgggcactc ctgggtctcg accttgcgcg agccgatcgt cgcgacggtg
     1861 cggcgcgcgg cctcgatgcc gaccgcctcg gcgggctcga gctcgccgag gaaccgcgac
     1921 ccggaccacc agtagccgtt gcgcttcttg gggttggtcg gatccgtggt gtcgtcggcg
     1981 agcggctcga cgaacagcga cgcgtagctg ccgcggtagc cgccgacgaa gccgccgctg
     2041 gtcgcgaacc cggtcgcgcc cagcacgcgc gaccagatcg cgccctcgga gttggtcacc
     2101 cgcgggtcgt gcttgcgcgc cgcggactcg ccggcgatcg cctggcgcag cgcccacgcg
     2161 gcgtcgacct cggcgacccg cgggtcgtag aggtcgaggt cggggacgtc cttggcgagc
     2221 agcgcgggat ccggcggcag cgcgtactcg tcgacctcgg ccaggtcggc cagggtcacg
     2281 ctctcggcgc acagcgcctc cagcgcctcg cgccgcaggt cgctggtgta cgtcaccgcg
     2341 cgccggccgc cgcgcagcac gcgcagcccg agcgcgcggc tgcccgcctc ctggaccagc
     2401 tcgggctcgc cgagccggac cttgacggtc agctcgttgc cttcgcggac cagggcctcg
     2461 gcgtcgtcgg cgcctgcccg gcgggcgagc tcgacggcga gctgtgcgat ctcgcgcagc
     2521 tcctgcacgg tgcggtcggt cacgccggcg gcgcccgaca tcgatcgcgc tgcggtcatg
     2581 cctgggtgcc ccccaccgtg atcgaggcga tccggatcgt cggggtgccg acgttgaccg
     2641 gcacgctctg gccgtccttg ccgcagctcc acacgccgtc ggacagctgg aagtccgagc
     2701 cgaccatgtc gacccggcgc agcacgtccg ggccgttgcc gatcagctgg gcgcccttga
     2761 gcggcgcggt gagcttgccg tcctcgatca ggtagctctc cgacagcgag aacacgaagt
     2821 cgccgttggt gatgttgacc gagccgccgg agaacccctt ggcaaagatc ccgcgcttga
     2881 cgctcttgac gatgtcctcg gggttgtcct tgccgccgag cagcgcggtg ttggtcatcc
     2941 ggggcagcgg catcgcgcgg tacgactcgc ggcggccgtt gcccgtgggc gccccgccga
     3001 agtgcttggc gctcagccgg tcgtgcatgt agccgaccag cacgccgttc tcgatcagcg
     3061 tgttgctctg gcccgggtag ccctcgtcgt cgacgttgat cgcgccgcgc gagttgccga
     3121 tcgtgccgtc gtcgaccacg gtgcacagcg tcgacgcgac cagcttgccc atctgacccg
     3181 agtagttcga ggtctgcttg cggttgaagt cggcctccag gccgtggccg accgcctcgt
     3241 ggagcaggat ccccgagtcg ccggcggcga gcacgaccgg catctcgccg gccggcacct
     3301 cgcgcgcgtc gagcatcgcg atcgcgaccc gggccgcctc gcggccgtgc gactccgcgt
     3361 cgcgctccgc gagctggaag tactccatgc cgtagcggcc gccgccgccg cccgagccgc
     3421 tctgccgctt gtcgttgcgc tcggcgaccg cgcgcacgcc gtaccggatc agcggctgga
     3481 ggtcggacgc catccggccg tcgctggtga acaccagcat ctccttgatg acctcggaca
     3541 ggaacgcctc gaccttgacg atccgcggat cgtacgcgcg cgcggcccgg tcaccggcgc
     3601 gcagcagggc gagcttgtcg tgcgccgaca ccatgagcga tggcatcggc accccgtaga
     3661 agctgggcac cgcgaccgtc gagatcggcg cgacgccgac cggcgaatgg ccgccgccga
     3721 ccgcgatctg gccggcggtg cgcgcggcgt gcgccatgcg gtcccacgcc agatcctcgc
     3781 agtacgcata gccggtggca tcgcccttgg tcacgcgcac gccgaggccg agcgtgatgc
     3841 cgcggccgag cgcgcggatc tgctcctcct cgagggcgta gtccgccgag acgcggaact
     3901 cgaagtagag gtccgcatag tcgccgccgg cctcgagcgc gatgctgagc aggcggtgag
     3961 ccagctgggc atcgatcgcg ttgccgccgc ccggggagaa cggggcgcgc agggccgcgg
     4021 ggagcgaggt gatcatgagg ctcactgtag cgcggcgatc ctcgcaaggc ccctcctcga
     4081 acggcgagtt tcgtcggtcc ggtgatgacc tacattccag gatccgacgc gcccgcgcct
     4141 cgtcgcgcga cacccggcgg cggcctcacc ggcggaacgc gggcggagcg cggagcggaa
     4201 cagggtggcg gcgcacggtg ccttcatggg ctccagattc agtgttggcg accacggatc
     4261 tgcggatcgg tgtttctcca ccggcgtgcg gcccaccggc gtaaccaccg caggttccag
     4321 cggtgttcgg cggcggagcg ccccgggcgc caccgcctgc ggcgctgccg ctccatgcag
     4381 caccgtgcga cgccgagtgc tacccgcgag cgtcgcgccc ggacagcttg gccagcgccc
     4441 cctcggccgc ctcgacggcc gcctggatct cggcctccgt gccgccgagc cagagcctgc
     4501 cgaacgcgcc gaacgtgatc acttccagca ggtggatgtt cgcagccttc tcggcctcgt
     4561 tggcggcgag cgccgcgtac gccgccgggt gaacctccag gatgtacagc gtctggccgg
     4621 acaggatcat gtcgccgtgg cgcatgcggt tgatcagctg gctctgcttg ccgtcgatcc
     4681 ccgtgaggat ctgcgacgac acgatccgcg gcttgatccg gtcctcgcgc tggaccgcca
     4741 tgcgcgccac gatcgcgtcg gccgcggcct cgacctggcc cttgtcgccg tgatggagct
     4801 ccagcatgcc gtaggcgcgc tcgacgatct gcatgccggg ccggcaccgg gtcgccttga
     4861 gcgcggcgtc ggtcagctgg ttgatcgcga tcccgggcgc gatctcgagg aacagcgcgg
     4921 cctcttcctc cagcggcatg aacccggagc acaccgtctg caagaagccg gtgagctgcg
     4981 gctgcagcga gtcgaggacg gtcagcgcgc gaagctccat cgcggccacg ctatcacagc
     5041 cgcgtagggc gcggccgcgc gcgaccggcc gccgaatgtc gactgccgtt ccgcgatccg
     5101 cccccacgtg ggggcgacga accggctgat gcgattccgc ggaatcgcca gctcgagacg
     5161 cttcgcacaa aggctgtcga ggcctgcctc aggcgcctcg cccagctgac gtcgaccggg
     5221 ccttgacctt gacggccatc gttgaggttg acgtagccgt ttgatgatgc gtcaccagtc
     5281 ggggacgttg ggcatggcgt ggaccttgac ctcagccgtt gaggttgacg gcagcgaagc
     5341 ggtacgtaga cgtctgaccc caccgtcgac ctcgaccgcc ttgccgcgct ctgtctaagc
     5401 tcgcagcgca catcggtcca cgtccaacct cgacggtgga ctcaacgtcg tacatcgccg
     5461 tcgacgacaa cgcttgcggt caacgatcaa gtcgacgtcg acgatcacga tgccaccgac
     5521 gaggagttgc gacgaatcat cagacgtcgc cgtcgacgtc caggttctgg tcaaggtcat
     5581 cgtcgaggac aaggtccaga ggatccagaa ccggcctcag ccttcttcgg tcgacacttc
     5641 ttcagaagcc gcctcgacag cgctgtgacc cagatccggc accgcgtgac cacccgatgg
     5701 cctcgcgacc cacgtcgggc ggatgcatgt ggacgtcgtg ctgcgccgcg tcctcgcaca
     5761 cgccacccga acgacgcggc tagttcgatg gacgcccgcg acgcggcgcg cggggcc
//
LOCUS       DASVOR010001822         1604 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_457225,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001822 DASVOR010000000
VERSION     DASVOR010001822.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457225"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VF516_33850"
     CDS             <1..1375
                     /locus_tag="VF516_33850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112781.1"
                     /translation="LGLACPTASAAPAAAPTAAPAAPSAPGTPAATGRIERVEHRDPV
                     SAPSRGPTHALVTVEVFFVPGQSMPVGALRLLEQLQDRHPARIRLIYRVLKSGSQLLV
                     PSAALEAYTEGKFFELMDELGKQRSALRLEDLQELARRVGVDPQRVAVATRLDHYREV
                     LEANQRRFERLHGGATPSALFNARATRVALGAITATDLDREYEAAYERALDKLDRGFA
                     PDQLAAAFDDEMIRSAQPVVMSVVGEDDDRSPLDQPLATPPLRLDGLPSFGKPGIAAA
                     VAVVLLCRPNDPACANLLRVAEPAVRLYPDDVRVVWAPWFDVGRDDAAELTLLGDATL
                     CAEAIGSNQGELTTSPGWVWVKEMYAQVARGRGKKVIGAQLIDDVSAKLDVDTRALSA
                     CRARLAGATLAWIAEARRSGVPRSNAAVVIGGRVYTGLSDPTLIQELVEAELAPGVLG
                     SLPRWHR"
BASE COUNT          211 a          603 c          572 g          218 t
ORIGIN      
        1 gctcggcctg gcctgcccca cggcgagcgc agcgccggct gccgccccga ctgcggcccc
       61 cgccgctccg tccgctcccg ggacccccgc cgcgaccggc cgcatcgagc gcgtcgagca
      121 ccgcgatccg gtgtcggccc catcgcgcgg acccacccat gcgctggtca ccgtcgaggt
      181 gttcttcgtg ccggggcaga gcatgccggt cggggcgctg cgcctgctcg agcagctgca
      241 ggaccgtcac ccggcgcgga tccggctgat ctaccgcgtc ctgaagagcg gctcgcagct
      301 gctggtcccc agcgcggcgc tcgaggccta caccgagggc aagttcttcg agctgatgga
      361 cgagctcggc aagcagcgca gcgcgctccg gctcgaggac ctgcaagagc tcgcccggcg
      421 ggtcggcgtc gacccccagc gcgtcgcggt cgcgacccgc ctcgaccact accgcgaggt
      481 gctcgaggcc aaccagcgcc ggttcgagcg cctgcacggc ggcgccaccc cgagcgcgct
      541 gttcaacgcc cgggcgaccc gcgtcgcgct cggcgcgatc accgccaccg acctcgaccg
      601 cgagtacgag gcggcctacg agcgcgcgct cgacaagctc gaccgcggct tcgcgccgga
      661 ccagctggcg gcggcgttcg acgacgagat gatccggagc gcccagccgg tcgtgatgtc
      721 ggtggtcggc gaggacgacg accgctcgcc gctcgatcaa ccgctcgcca ccccgccgct
      781 ccggctcgat ggcctgccgt cgttcggcaa gcccgggatc gccgccgcgg tcgccgtcgt
      841 cttgctgtgc cggccgaacg acccggcgtg cgccaacctc ctgcgcgtgg ccgagcccgc
      901 ggtgcggctc taccccgacg acgtgcgcgt ggtgtgggca ccgtggttcg atgtcggccg
      961 cgacgatgcc gccgagctca ccctgctggg cgacgcgacg ttgtgcgccg aggcgatcgg
     1021 gtcgaatcag ggcgagctca cgacctcgcc cgggtgggtg tgggtcaagg agatgtacgc
     1081 ccaggtcgcc cgcggccggg gcaagaaggt gatcggcgcg cagctgatcg acgacgtgtc
     1141 cgccaagctc gacgtcgaca cccgggcgct ctcggcgtgc cgggcccggc tggccggcgc
     1201 gaccctggcc tggatcgccg aggcccggag gtccggggtg ccgcgcagca acgcagcggt
     1261 cgtgatcggc ggccgggtct acaccgggct gtccgacccg acgctgatcc aggagctggt
     1321 cgaggccgag ctggcgcccg gggtgctcgg ctccctgccc cgctggcatc gctagcctgg
     1381 gaaccccgcc ccgtcctgga attgttgcca tgacgctggt ttgaccgtca gacccgcgcg
     1441 tgttacccga tgaaggtgga gctgacgttc gcggcagcga ccgacacagg tcggcagcgc
     1501 acgcacaacg aggacaactt cctcatcgac aagaagctcc gtctgttcct ggtcgccgac
     1561 ggcatgggcg gccatgccgc cggcgaggtg gcgtcgtcga tcgc
//
LOCUS       DASVOR010001823         7621 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_20259,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001823 DASVOR010000000
VERSION     DASVOR010001823.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7621
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20259"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5666)
                     /locus_tag="VF516_33855"
     CDS             complement(<1..5666)
                     /locus_tag="VF516_33855"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8112782.1"
                     /translation="MNTPIAIVGIGCRFPGASNDPEELWRLLTHGVDAIREIPADRWS
                     LRSYYHADPARPGRTYARWGGFLDCVDQFDAAFFSMSPREAAKADPQQRLLLEVAYEA
                     LEDSGQTIEGISGKRGGVFVGISSFDYAGIQCSRYERDSIDAYMNHGSALSIAANRIS
                     YFFNLHGPSFAVDTACSSALVAVHLACQSMWSGEIELAFVGGVNLMLRPEPTIGFSKA
                     SMLAPDGRCKSFDAAADGYARAEGAGIVLLKPLPKAQADGDSIYAVIRSTAANQDGRT
                     GGIAVPSADAQQAMLREALDHAGIAAQSVQYVEAHGTGTPVGDPIEAAAIGAVYGAPR
                     PPGQECLIGTIKSNFGHPEAASGAAGLIKAALAVQRGAIPATLHQVNPNPHIPFDDLK
                     LRVVNELTPWPDTGGEARRAAVNSFGFGGTNAHVILESAPAVEPRRSPPADGTGIARP
                     MLLPISARSPEALRAAAATYAQFLQDDTRAGASLADICHTAAVRRSHHDHRLAVVAHT
                     TAEFCDLLRGFLAGESSSMCHAGRQSSKHGVAFVCSGMGQQWWAMGRQLLAEERGYRD
                     VVGHIDEILRALAGWSLLREFEADEQHTRINETRVAQPAIFATQVGIAAVLKQWGIQP
                     SALVGHSVGEVAAAYLAGALSLEDAVKVTYHRSRLQQRTAGLGTMLAAGVSWQEAHRL
                     INGYDAVVSVGAINSAESVTLSGDSEVLRAIAQELDAKGVFCRALNVEVPYHSPTMDT
                     LRSELLDSLGDLRPRATSVPLYSTVTGGLIDGTTLTATYWWQNVRQPVQFHQATSSAI
                     EAGHDVFMEIGAHPVLSVSIAECLREHDRKGVVVPSLRRGQPENATLLASAGRLYTLG
                     ATIDWHGVTRSEGTFVKLPTYPWQRERHWHESDRARRERLGEVVHPLLGMRSESAEPR
                     WLVELDLASLPFLSDHRAQGATIFPAAGYVEMGLAVGHELLGAAPCVVEDLEFRKALF
                     LSDNAPSTIEVAVQRSSHTFEVSTLTPGSDIWTGHAGGKVRALPAAALRKPLDLDAIR
                     ARCPNAISGKECYRRFQALGLEYGPAFQPIAQLWRGADEAIAELHPRNVGDDQDEFWL
                     DPALLDGLFQVSLGTLAERQETGLYLPVRIDRVEYHAPPGARTFGYARVRERNGAEST
                     GDLVLTDEQGVRLVEVRGFTCRFMETGKETSNWPVYQYQWRLKTRVTKRPADVRSAAH
                     LPRPSELEPVLAAEAAVLRQRWRRDKFHGERGRLLCDMAVAYIFEAFTELGWDPVRDA
                     AIPVAALAEQLGVVPEHRRLFARLLEGLTSEDISAAPGVLGPSRRRAIDVWRQIWRQY
                     PDAMPELTLISSCGGHLAGVLRGDVNPLEVIFPEGSSVTAEHLYQDSPVYRVYNQLIQ
                     KALSEIVQRLPEGRTLRVLEIGGGTGGLSAFVLPKLPPLRTQYTFTDIGATLVSQAER
                     NFSHYPFIEYRTLDIDANPVEQGFSPHVYDIIVASDVLHATRNLGRTVGHVAQLLASE
                     GTLLLLEPENPELAMTLAFGMLEGWRQSADTELRWDEPLITHGAWRRLLAKSGFEESV
                     VITDAPTEEQARHAVIVARGPSVDATSSMTVRDRRASWLVFADRAPDGLGTGESVAAS
                     LRRCGQDVVMAYAGTTFRQREDGAFVVAPGTPADIDRLFDALPCNVSELAGIVHLWSL
                     EAPSSDEADGDGMERAWRVGLMSGLQLAQALGARAGSLPPLWLVTRGAQAVGGDPLAS
                     SVAQSVLWGLGVTVVNECPQFRTHLVDLGTGGPEEIRSLVDELLDEDDEDQIALRGDA
                     RYIRRLLHLPKADGAPVIARAATPRPDFRIEAMIPGILDTLGPREVIRRPPGPGEVQI
                     EVVAAALNFKDVMMAMALLPPEATRGGYSLGMLGLECSGRITAVGDG"
     gene            5941..6798
                     /locus_tag="VF516_33860"
     CDS             5941..6798
                     /locus_tag="VF516_33860"
                     /inference="COORDINATES: protein motif:HMM:NF015915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8112783.1"
                     /translation="MSDSDDVLDFKPPPVEALWRLFAPLRAYHRPRFFGLENIDPKQP
                     SLLVGNHTIFGLDIPHLGVAIYRERGVILRSLGDRAHFRIPGWGELLKSLGAVVGTRE
                     NCAWLMQSGAHVLIFPGGTREVYKRRGEAYKLLWKERTGFTRMAIEHSYPITPFACVG
                     AEECYDILVDANDVMKSPLGKFLDRTGITQRFLRGGEAIMPIARGLGFTTIPRPERFY
                     FQVGAPIDTRRFAGKHDDKAALLALREEVRVAVESLIEKLLTIRAKDPKRTLLGSFLS
                     RSGDDDQQA"
     gene            7302..>7621
                     /locus_tag="VF516_33865"
     CDS             7302..>7621
                     /locus_tag="VF516_33865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112784.1"
                     /translation="MQLAFVALDPEGFLRRRAREGDPFRVRLPFAGEGLVTGSPEGAR
                     ELFTADPAICEIMPANPLEGLLGPGSLILKSGAVHARERKLLLPAFHGERMRAYAEVI
                     EDATA"
BASE COUNT         1352 a         2520 c         2431 g         1318 t
ORIGIN      
        1 ccgtcgccta cggcggtgat tcgccccgag cattcgaggc cgagcatgcc gagcgagtag
       61 ccgcctcggg tcgcctccgg cggcaacagg gccattgcca tcatcacgtc cttgaagttc
      121 agtgcggccg ctacgacttc gatctgtacc tcgcctggtc cgggcggacg cctgatcact
      181 tcgcgcggcc ccagcgtgtc gaggatgccc ggtatcatcg cctcgatccg gaagtccggc
      241 cgaggagttg ccgcgcgcgc aatcacggga gcgccgtcgg ccttcggcag atgcagcagg
      301 cggcggatgt acctcgcatc gccacgcaac gcgatctgat cctcatcgtc ttcgtcgagg
      361 agttcgtcga cgaggctgcg gatctcctcg ggaccgccgg ttccgagatc gacgagatgg
      421 gtccggaact gcgggcattc gttgacgacc gtgaccccca gcccccacaa gacggattgc
      481 gccaccgaac tcgccagggg atcgcctccc accgcctggg cgccgcgcgt gacgagccac
      541 agcggcggca gcgatccggc ccgcgcgccg agcgcctgcg caagctgcag gccgctcatc
      601 aggccgacgc gccacgcgcg ctccatcccg tcgccgtctg cctcgtccga cgaaggcgct
      661 tcgaggctcc acaggtgaac gatcccggcg agctcgctga cgttgcacgg caacgcgtcg
      721 aacagccgat cgatgtccgc cggcgtgccg ggcgcaacga cgaacgcgcc gtcctcgcgc
      781 tgacggaatg tggttcctgc atacgccatc acgacgtcct gcccgcagcg cctgagagac
      841 gccgcgaccg attcgccagt accaaggcca tcaggcgccc gatctgcaaa gaccagccac
      901 gaagcacgcc tgtcgcgaac cgtcatcgag ctcgtagcat cgacgctggg gccacgtgca
      961 acgatgactg cgtggcgtgc ctgttcttcc gtgggcgcat cagtgatgac gaccgattcc
     1021 tcgaagccag acttggcgag caggcggcgc caggcgccgt gtgtgatcaa tggctcatcc
     1081 caccgaagct cggtgtcggc ggactgccgc catccctcga gcatgccgaa cgcgagcgtc
     1141 atcgccagtt cagggttctc cggttcgagc agtaacagag tgccttcgga cgcgaggagc
     1201 tgtgcaacgt ggcccaccgt gcgaccgaga ttccgtgtcg cgtgcagcac gtcggacgcg
     1261 acgatgatgt cgtagacgtg cggactgaat ccctgttcta ctggattcgc atcgatatcc
     1321 agtgtgcggt actcaatgaa cggataatgg ctgaagttcc gctccgcctg tgacacgagg
     1381 gtcgcaccga tgtcggtgaa ggtgtactgg gtgcggagcg gcggaagttt cggcagcacg
     1441 aacgcagata gaccgcctgt gccgccgccg atctcgagta cgcgaagtgt ccgtccctcg
     1501 gggaggcgct gaacgatctc ggagagggcc ttctggatca gctggttgta gacgcggtag
     1561 acgggcgaat cctgatacag gtgttccgcg gtgaccgatg atccttccgg gaagatcacc
     1621 tcgagcgggt tcacgtctcc gcgcagaacg ccggccaggt gaccaccaca cgagcttatc
     1681 agggtgagct ccggcatcgc gtccggatat tgtcgccaga tctgccgcca gacatctata
     1741 gcacgacgac gagagggccc gagcacacct ggcgccgccg agatgtcctc actcgtcagc
     1801 ccctccagca accgcgcgaa cagccgccgg tgttcgggta ccacacccag ctgctccgcg
     1861 agcgcagcca cggggatagc tgcatcccgg accggatccc atccgagttc ggtgaacgcc
     1921 tcgaagatgt aggcaactgc catatcgcac aacaggcgac cgcgctctcc gtgaaatttg
     1981 tcacgcctcc agcgctgccg caggactgcg gcttccgcgg cgagcaccgg ctccagttcg
     2041 ctcgggcgtg gcaggtgcgc ggccgaccgc acgtccgccg gccgcttggt gactcgcgtc
     2101 ttcaaccgcc actggtactg atagaccggc cagttggacg tctccttgcc tgtctccatg
     2161 aaacggcacg taaagccacg cacctccacg agtcggacac cctgctcgtc ggtcaggacg
     2221 agatcgcctg tggactcggc gccgttacgc tcccggacac gcgcgtaccc aaacgttcgc
     2281 gcacctggcg gtgcgtgata ctcgacgcgg tcgatacgca ccggaaggta gaggccggtc
     2341 tcctgccttt cggcgagcgt gccgaggctc acctgaaaga gcccatcgag gagcgccggg
     2401 tccaaccaga actcgtcttg gtcgtctccg acattgcgag ggtgcaattc tgcgatggcc
     2461 tcgtcagcgc cgcgccagag ctgcgcgatt ggttgaaacg ccggaccgta ctcgagtcca
     2521 agcgcctgaa agcgccggta gcactccttg cctgagatcg cgttcgggca gcgcgcccga
     2581 atggcgtcga ggtcgagcgg ttttctcagc gcagctgccg gcaacgcgcg caccttgccg
     2641 ccggcatgtc cggtccagat gtcggatccg ggcgtcagcg tggatacttc gaaggtgtga
     2701 gatgaccgct gcacggcgac ttcgattgtc gaaggggcat tgtccgagag gaagagcgcc
     2761 ttccggaact cgagatcctc gacaacgcag ggggctgcgc cgagtagctc gtgcccaaca
     2821 gcaagcccca tctccacata tcccgccgct ggaaagatcg tcgcgccctg ggcgcggtga
     2881 tcggacaaga atggcagcga cgcgaggtcg agttcgacaa gccagcgcgg ctccgccgat
     2941 tcgctgcgca taccgagcag gggatgcacg acttctccca gccgttcgcg acgggcccga
     3001 tccgattcgt gccagtgacg ttcgcgctgc cacgggtatg tcggcagctt gacgaatgtt
     3061 ccctcggagc gagtgacgcc gtgccagtcg atcgtcgcac cgagcgtgta gagacgtcct
     3121 gccgacgcga ggagcgttgc gttctcgggt tgtccgcgtc ggagcgacgg gaccacgacc
     3181 cccttgcggt catgctcgcg aagacactcg gcaatcgata cactgaggac cggatgggcg
     3241 ccgatctcca tgaacacgtc gtggccggcc tcgatcgcgg aggacgtcgc ctgatggaat
     3301 tgcaccggct gtcggacgtt ttgccaccag tacgtcgctg tgagcgtcgt gccgtcgatc
     3361 agcccaccag tcaccgtcga atacaacggc accgaagtgg ctcgcggtcg aagatccccg
     3421 agcgaatcca gtagctccga cctgagcgtg tccatggtcg ggctgtggta cggcacctcc
     3481 acattgagag cccggcagaa gacgcctttg gcgtcgagct cctgcgcgat cgcgcgaaga
     3541 acttcggagt cgccagacaa ggtcaccgac tcggcgctgt tgatcgcgcc gaccgatacg
     3601 acggcatcat aaccattgat gagtcgatgg gcctcctgcc atgacacacc ggcggcgagc
     3661 atggttccca ggcctgccgt gcgctgctgc aggcgacttc ggtgataagt gaccttcacg
     3721 gcgtcttcga gcgacagagc accggcgaga tacgcggcgg ccacttcgcc aacgctgtgg
     3781 cccacgagcg ctgaaggctg gattccccac tgtttcagga cggccgcgat ccccacctgc
     3841 gtcgcgaaga tcgcgggctg cgcgacgcgt gtttcgttga tccgggtatg ctgttcgtcc
     3901 gcttcgaact cgcgcaggag agaccaccca gcgagcgcgc ggaggatctc gtcgatgtgt
     3961 ccaacgacgt cgcgatagcc acgctcctca gcgagcagct gccggcccat cgcccaccac
     4021 tgctgtccca tgcccgaaca cacgaaggcc acgccatgct tggacgattg gcggccggcg
     4081 tgacacatcg agctgctctc acccgccaga aatccgcgca acagatcgca gaactcggct
     4141 gtggtgtgcg cgacgacggc gagccggtga tcgtggtgcg atcggcggac ggcggccgtg
     4201 tgacagatat ccgccagtga ggcgccggcg cgcgtgtcat cctggaggaa ctgtgcgtac
     4261 gtggcggcag ccgcacggag cgcctcaggg ctcctcgccg agatcggaag cagcatcggc
     4321 cgggcgattc ccgtcccgtc ggcaggaggc gaccggcgcg gttcgaccgc aggggcggat
     4381 tccaggatga cgtgtgcgtt ggtcccacca aagccaaagg agttgaccgc cgcgcgccgt
     4441 gcctccccgc ccgtgtcggg ccacggcgtc agttcgttga cgacacgtag cttcaggtcg
     4501 tcgaacggaa tatgggggtt ggggttcacc tggtgcagcg tggctggaat cgcgccacgc
     4561 tgtaccgcaa gcgctgcttt gatcaggccc gcagcaccgg atgcggcttc gggatgacca
     4621 aagttgctct tgatcgtccc gatcaggcat tcctgtcccg ggggacgagg ggcgccgtac
     4681 accgctccga tagcggcggc ttcgatcggg tcgccgactg gcgtgccggt accgtgtgcc
     4741 tcgacgtact ggacgctttg cgccgcgatg cctgcgtgat cgagcgcctc ccgcaacatc
     4801 gcctgctggg cgtcggcgct tggcaccgcg atgccgccgg tacggccatc ctggttcgcg
     4861 gccgtgctcc tgatgacggc gtagatggaa tcgccgtctg cctgcgcctt cggcagcggc
     4921 ttcagcaaga caatgccggc accttcggcg cgcgcatatc cgtcggccgc cgcatcgaag
     4981 ctcttgcatc gtccgtccgg cgcgagcatg gacgccttgc tgaaaccgat ggtcggctca
     5041 ggccgcagca tgaggttcac gcccccgacg aacgccagct cgatctcgcc gctccacatg
     5101 ctctgacagg cgaggtgcac ggccacgaga gcagacgagc aagccgtgtc cacggcaaag
     5161 ctcggcccgt gaaggttgaa gaagtacgag atgcggttgg ctgcgatcga cagtgccgag
     5221 ccgtgattca tgtacgcgtc gatgctgtca cgctcatatc tggagcactg gatgccggca
     5281 tagtcgaaac tcgagattcc gacgaacacg ccgccacgct tgcccgagat cccctcgatc
     5341 gtctgcccgg agtcttcgag tgcctcgtac gcgacctcga ggagcaatcg ttgctgcgga
     5401 tcggctttgg cggcttcccg tgggctcatc gagaagaatg ctgcgtcgaa ctggtcaacg
     5461 cagtcgagaa aacctcccca tcgcgcatac gtgcgtccgg gtcgcgcagg atcggcgtga
     5521 tagtatgacc gcaaactcca tcggtcggca gggatctccc tgatggcgtc gaccccgtgt
     5581 gtcaacaagc gccagagctc ctcggggtcg ttcgatgctc ctggaaatcg acacccaata
     5641 cccacgattg caataggagt attcattgtc tttccgcgaa gagcatactc tgcgatattc
     5701 ttgcgtcaat tctcaaatga agtcgcagtc tcgcctcgcc tcttctagca tcgatttgcc
     5761 cgagtctgcc accctgaagg ggtactgcgg ataggtttcc agaatacatg cgacttcact
     5821 acaagctaag gtcgctcatc gcgcattctc gagctgcagc cgccggcgca ggacgtggat
     5881 caggagagtc ccagatgtga tgaacgccag gataaatgag agctgatgta tactggccgc
     5941 atgagcgact cggacgatgt gctcgacttc aaaccacccc cggtcgaggc gctatggcgg
     6001 ctcttcgctc cactgcgcgc gtatcaccgg ccgcggttct tcggcctcga aaatattgac
     6061 cccaagcagc cgagcctgct cgtcggcaac cacacgatct tcggcctcga catcccgcat
     6121 cttggcgtcg ccatctaccg cgagcgcggc gtcatactgc gtagccttgg cgatcgggcc
     6181 cactttcgga tcccgggctg gggcgagctg ctaaagagcc tcggcgccgt cgtcggcacg
     6241 cgcgagaact gcgcgtggct catgcagagc ggcgcgcacg tcctgatctt tccgggggga
     6301 acccgcgagg tctacaagcg caggggcgag gcctacaagc tcctgtggaa agagcgcacg
     6361 ggctttacgc gcatggccat cgagcacagc tatccgatca cgccctttgc gtgcgtggga
     6421 gcggaggagt gctacgatat cctcgtcgat gccaacgacg tgatgaagtc gccgctcggc
     6481 aagttcctcg accgcaccgg gatcacccag aggttcctgc gcggcggcga ggcgatcatg
     6541 cccatcgccc gcggcctcgg cttcacgacg atcccgcgcc ccgagcgctt ctatttccag
     6601 gtcggcgccc ccatcgacac ccgtcgcttc gccggaaaac acgacgacaa agcggcgctg
     6661 ctggcgctcc gcgaagaagt ccgcgtggcc gtcgagtcac tcatcgaaaa gctccttacg
     6721 atccgcgcca aggatcccaa gcgcacgctt cttggcagct tcctgagccg ctccggcgac
     6781 gacgaccagc aagcctgaaa cccaatccaa cccccgccga gggaggaaat tcaatctcac
     6841 cacgtctcct ggaaagggcg cgactcgacc tcgaaggacc atgacgatgg ctgctgctgt
     6901 gtgagctggt gggctcgagc tacgcacatc ggaagtccgt gcgatgccgc gcacccgtac
     6961 ggaggcggca gatcaccggt ccagggtcta cgtcgcctca tccgcgggca gtgggacaac
     7021 tcgtgcgaca ccgcgtgcgc aagactctca tggcgaagtg gtgtagcccc atcggtagag
     7081 cctagctggt agtcctgatg ccacgcctcc ggcgcgaggt gggcgtacat ccagaatcgg
     7141 ccctcgtccg gatccgcttg tatgcgggtc cgggacctcg cgctacttca cgccgcggca
     7201 gatcaggaac cggtggggaa cttctcggtc acgtcgctcg gaagcatggc ggggcgggat
     7261 cacaagggta tggtccgcgt gtcatgaggt tgcctggagt cgtgcagctg gcgttcgtcg
     7321 ccctggatcc cgaaggattc ctccggcggc gcgcgcgcga gggcgatccc ttccgggtgc
     7381 gcctgccctt cgcgggcgag ggcctggtga ccggcagccc cgagggcgcg cgcgagctct
     7441 tcaccgcgga tcccgcgatc tgcgagatca tgccggcgaa ccccctcgag ggcttgctcg
     7501 gcccgggatc gctcatcctg aagtccggcg cggtgcacgc gcgcgagcgc aaactgctct
     7561 tgcccgcctt ccacggcgag cgcatgcggg cgtacgcgga ggtcatcgag gacgcgacgg
     7621 c
//
LOCUS       DASVOR010001824         2452 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_107541,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001824 DASVOR010000000
VERSION     DASVOR010001824.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107541"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2452
                     /locus_tag="VF516_33870"
     CDS             <1..>2452
                     /locus_tag="VF516_33870"
                     /inference="COORDINATES: protein motif:HMM:NF023874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair ATPase"
                     /protein_id="HEX8112785.1"
                     /translation="AVFQAGATWRDCKVLRWRIEPDGQVIYVDDRGDRDHAALYPPAY
                     DFEWVEVTRDHQVSGKHPHYNVLDTLFVECVHGDLTIKVENNTETGQGVYAEPVDDPN
                     QSLDDARIHYAPIGRPGGLIVLKVLPFRETAWRYLVFDTRSQTVVRADGIGQGCRSLP
                     EDHGVIFPGGTVLVGGARKMFDGDASDYVLERELRSPNGEDVLYVYFRGSDGTYLLYS
                     YNLIDKAVAAPIPCHGYSLFGDGRMIVMRASAEPTRVHPMQVWRTPFTSAEHAASAPT
                     DGSYLAKVGNADLVRGLSDALSIARLAGADAPVRTTFDDILALATRAIDQYYWLGHAD
                     AGDLRTAIADVKTTARLILDEYDKVVALQAEARRVLAALEAQVAERLRATRSEGFDRI
                     EPFLDALTGLRALRGELITRQDVRFIDTARLAALEAEVAAAFDRVTMDCARFLVRGDA
                     FGELIARADGLAARVGELATAAPVKEIRDQIDQVAGGVDLLGNVIGALQIADTTQRTQ
                     ILENITDAFGRVNRARAAIDVRYRELAAREGRAEFAAQVKLLTQSVASSLAQCDTPER
                     CDRELARLLVQLEELEGRFADIDELTAELAAKREDIAQAVGTRKQQLAAERQRRAANL
                     DKAAQRILEGIARRSVQLASADEVNAYFAADAMVGKLRDIGRELAALGEAVKADELDA
                     RLKAARQDALRGQRDRAELFEGQGELIRLGDHRFAVNTQPLELMIVPHDGGLAVHLTG
                     TDFYEPVTDDRLLAARGLWDRDLPSESPEVYRGEYLAASILADAEAGLEAVTPGAPPL
                     AALHDAARAGALEPMVRAV"
BASE COUNT          350 a          903 c          890 g          309 t
ORIGIN      
        1 cgcggtgttc caggccggcg cgacctggcg cgactgcaag gtgctgcgct ggcggatcga
       61 gcccgacggc caggtgatct acgtcgacga ccgcggcgat cgcgatcacg ccgcgctgta
      121 cccgccggcc tacgacttcg aatgggtcga ggtcacgcgc gaccaccagg tgtcgggcaa
      181 gcacccgcac tacaacgtgc tcgacacgct gttcgtcgag tgcgtgcacg gcgacctgac
      241 gatcaaggtc gagaacaaca ccgagaccgg gcagggcgtg tacgccgagc cggtcgatga
      301 tccgaaccag tcgctcgacg acgcgcggat ccattacgcg ccgatcggtc ggcccggcgg
      361 cctgatcgtg ctcaaggtcc tgccgttccg cgagaccgcg tggcgctacc tggtgttcga
      421 cacccggtcg cagacggtgg tgcgcgccga cggcatcggc cagggctgcc gcagcctgcc
      481 cgaggaccac ggcgtgatct ttccgggcgg caccgtgctg gtcggcggcg cccgcaagat
      541 gttcgacggc gacgccagcg actacgtgct ggagcgcgag ctgcgctcgc ccaacggcga
      601 ggacgtgctg tacgtgtact tccgcggcag cgacgggacg tacctgctgt actcgtacaa
      661 cctgatcgac aaggcggtcg cggcgccgat cccctgccac ggctattcgc tgttcggcga
      721 cggccggatg atcgtgatgc gggcgtcggc cgagccgacc cgggtccacc cgatgcaggt
      781 gtggcgcacg ccgttcacct cggccgagca cgccgcgagc gcgccgaccg acggcagcta
      841 cctcgccaag gtcggcaacg cggatctcgt gcgcgggctg tccgacgcgc tgtcgatcgc
      901 gcggctcgcc ggggccgacg cgccggtgcg cacgacgttc gacgacatcc tcgcgctggc
      961 gacccgcgcg atcgaccagt actactggct cggccacgcc gacgccggcg atctgcggac
     1021 cgcgatcgcc gacgtcaaga ccacggcgcg gctgatcctc gacgagtacg acaaggtggt
     1081 cgcgctgcag gccgaggccc gccgcgtgct cgccgcgctc gaggcccagg tcgccgagcg
     1141 gctgcgcgcg acccgctcgg aggggttcga ccggatcgag ccgttcctcg acgcgctgac
     1201 cgggctgcgc gcgctgcgcg gcgagctgat cacccggcag gacgtccggt tcatcgacac
     1261 cgcgcggctc gccgcgctcg aggccgaggt cgccgcggcg ttcgaccgcg tgaccatgga
     1321 ctgcgcgcgg ttcctggtcc gcggcgacgc gttcggcgag ctgatcgcgc gcgcggacgg
     1381 cctggcggcg cgggtcggcg agctcgccac cgcggccccg gtcaaggaga tccgcgacca
     1441 gatcgaccag gtcgccggcg gggtcgatct gctcggcaac gtgatcggcg cgctgcagat
     1501 cgccgacacc acccagcgca cgcagatcct cgagaacatc accgacgcgt tcggccgggt
     1561 caaccgcgcg cgcgccgcga tcgacgtccg ctaccgcgag ctcgccgccc gcgagggccg
     1621 cgccgagttc gccgcccagg tcaagctgct gacccagagc gtggcgtcgt cgctcgcgca
     1681 gtgcgacacg ccggagcgct gcgatcgcga gctcgcgcgc ctgctggtcc agctcgagga
     1741 gctcgagggc cggttcgccg acatcgacga gctgaccgcc gagctcgccg ccaagcgcga
     1801 ggacatcgcc caggcggtcg gcacccgcaa gcagcagctc gcggccgagc gccagcgccg
     1861 cgcggccaac ctcgacaagg cggcgcagcg gatcctggag ggcatcgccc ggcgcagcgt
     1921 gcagctggcg tcggccgacg aggtcaacgc gtacttcgcg gccgacgcga tggtcggcaa
     1981 gctgcgcgac atcggccgcg agctcgccgc gctcggcgag gcggtcaagg ccgacgagct
     2041 cgatgcccgg ctcaaggcgg cgcgccagga cgcgctgcgc ggccagcgcg atcgcgccga
     2101 gctgttcgag ggccagggcg agctgatccg gctcggcgac caccgcttcg cggtcaacac
     2161 ccagccgctc gagctgatga tcgtgccgca cgacggcggg ctcgccgtcc acctgaccgg
     2221 caccgacttc tacgagccgg tcaccgacga ccgcctgctc gccgcccgcg ggctgtggga
     2281 ccgcgatctg ccgagcgagt cgcccgaggt ctatcgcggc gagtacctgg cggcgagcat
     2341 cctcgccgac gccgaggccg ggctcgaggc ggtgacgccg ggcgcaccgc cgctggccgc
     2401 gctgcacgac gcggcgcggg cgggcgcgct ggagccgatg gtccgcgcgg tc
//
LOCUS       DASVOR010001825         6644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_427512,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001825 DASVOR010000000
VERSION     DASVOR010001825.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6644
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427512"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2469)
                     /locus_tag="VF516_33875"
     CDS             complement(<1..2469)
                     /locus_tag="VF516_33875"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8112786.1"
                     /translation="MQASEQPGARSGPPVRASASLDQWTGVRMPIQQFLPLAIRLTGE
                     LAALHARGAIHQDIRPSSVLFDAELGELALVDPPIAGSRAPALSAGSLPYIAPEQTGQ
                     MNRPIDSRSDLYSLGVVFYQLLAGRLPFEAEDAVGWVHCHVARRPRPFDQLRPPVPRQ
                     LIDIVGKLLAKLPDHRYQSALGLCHDLERCLRDWTEHGALLSFPLGERDVSEEFRIPQ
                     KLYGRDAECAALREAFERAAESGTPELIVVSGYAGIGKSSVVRELLRTVVQRRGRLIT
                     GKFEQYKRDIPYFTITQALRELALDILAEGELGIARWRQRFTQALGPHGQLIVDLVPQ
                     LGLIVGPQPPVPELPLTASETRLRRVFGRLFAACATPDHPLAMFIDDMQWADSASLAL
                     IASLLTDGNTHHLLIIGAYRDNEVDRSHPTVRALEAARRSHARIRDLVLGPLSEQDLG
                     RLVADTVHASLDEAAPLAGLVRDKTGGNPFFAIQFLTALHHKRSIWFDRDAYRWRWDA
                     ARVRAEGYTDNIAELMRSRLYALPSETQAALQGAACIGGTVDDATLTIACEREVAPAL
                     RPAIEQHLLFESVQADRCIYRFPHDRVHEAAYALVPEAERARVHLKIGRRMLASTPPE
                     ELPGKVFEIVSQLDHGLALIDAGPERERLAELHLLAGRRAQASTAYASALRYFTVGAE
                     LLAAAPEVRRPELAFALELHRAECELLTGAFDAAEQRLTGLAQRAAGIVDLAAVTSTR
                     IVLCATLDRAALAVEVTLEYLRRVGIDWPAHPADDEVRREYDRIWQQLGSRAIEELAD
                     LPAMTDPEQRATMDVLAHAQSPALV"
     gene            complement(2668..3981)
                     /locus_tag="VF516_33880"
     CDS             complement(2668..3981)
                     /locus_tag="VF516_33880"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8112787.1"
                     /translation="MTGSQPRLLRVVGLAGLTAIALNGVIGAGIFSLPATVYALAGAA
                     SPIAYLVAAVLMALVVACFAEAGSRSDETGGPYLYARDAFGDFVGFLVGWLFLLTRAV
                     ATAAIANTFVAYLGSVVPALATSPGRFAAITVAIGGLAAINVYGVRAASSTVNLLTIA
                     KLIPLLVFVGVGLAFVEPGRVALLGLPSGSLRQAALPLVFAYGGFENANVPTEEAVDP
                     RRHLPVALLTTIAAVAVLYILIQVVAQSVLPGLATSETPLASAAGAMMGAPGGWLLTA
                     AAIVATLGSISAVILVGPRVLYAFARHGQLPAGLAAIHPRYHSPHRAVIAFAVLTWAA
                     AVSGGFAQLVSLSAVSRLLFSAIICLAVPILRRRRELPPRFVLPGGPVIPLLAVALSI
                     WLLTGVSRAQAMAGGIGILAGLVVYAGHRWLWPELQRSPGRPPRE"
     gene            complement(3978..4961)
                     /locus_tag="VF516_33885"
     CDS             complement(3978..4961)
                     /locus_tag="VF516_33885"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8112788.1"
                     /translation="MRYDLHTHYYPASFFDLIRNTPGEFSFGTDPTGRTIIRHRGARF
                     FGVTAPMTDPALRLADMDRVGIDVEVLSLSTPNVFFAEAAGQAEVARVVNDAYADLIA
                     KHPTRFKGFASIPMDAPDAALRELDRALGELRLSGVVLLSNIRGRALTDPSYRPFFEE
                     ANRRRLCIFLHPMIPVASEVFNEFVLGPLVGFPFDTSLAVARMCFAGMFRDFPDIRWV
                     IGHLGGAIPYLMERLDNGYRDFAECRAHIDALPSTYLKRLHYDTVSFSGPALRMVRDL
                     VGVDHMVMGSDYPHMLGSIDRAVTSIEALDIAAHEKQSIFSGTALRILNNV"
     gene            complement(5016..6053)
                     /locus_tag="VF516_33890"
     CDS             complement(5016..6053)
                     /locus_tag="VF516_33890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112789.1"
                     /translation="MRRSIYLLALVACSNCSGPASDPAADEPASAIDVAALSGLGTVT
                     NVRNLANCPSGAPPGATCRQVTVTGCPGIATEPIDAVVAIRSAMGTLRGTVVHFIGGG
                     GEGFQGVNQGYEAAGFRNVYISWVSDWEQTQSSGIRTAACRPATILQWVFTEPTLHNS
                     SRSLGFCGEGFSGGSGQLGYALAHYGMGSVLDYVNELSGPPFGRIDIGCNGDAPPTAT
                     VCGVADTTRLPGSLNAWENIQPPLTCGSHNVPATELARWKSDSIDIGGVYNYPQTQVQ
                     FFACTFQATAVTVMGNNYFNTIVQAEGSNPNLAQYHCYFQSDGCQGEGLGTGLSDAAH
                     ALISNCVPRHQ"
     gene            6217..>6644
                     /locus_tag="VF516_33895"
     CDS             6217..>6644
                     /locus_tag="VF516_33895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112790.1"
                     /translation="MTHRWLAFAAVTLAAPAAFADRAVTGSMVDDATGLPVIGALVTV
                     GSGEAATDDQGQFRVDGVAFGRVDVVVIADGYRAYFGSARIGAVLAIRLEAAGASSEV
                     IRVSGRPPGGPPLHLDTAAVRTQPGAGNDVLRALQSLPGVA"
BASE COUNT          999 a         2339 c         2430 g          876 t
ORIGIN      
        1 gacgagcgcc ggcgactggg cgtgcgcgag cacgtccatc gtcgcgcgct gctcgggatc
       61 ggtcatcgcc ggcaggtcgg cgagctcctc gatcgcacgc gacccgagct gctgccagat
      121 ccggtcgtac tcgcgccgga cctcgtcgtc cgccggatgc gccggccagt cgatccccac
      181 gcgccgcagg tactcgagcg tcacctcgac ggcgagcgcc gcccgatcga gcgtcgcgca
      241 gagcacgatc cgcgtcgacg tcaccgccgc caggtcgacg atcccggcgg cgcgctgcgc
      301 gagcccggtg aggcgttgct cggccgcgtc gaacgcgccg gtcaggagct cgcactcggc
      361 gcggtggagc tcgagcgcga acgcgagctc ggggcggcgg acctcgggcg cggccgcgag
      421 cagctcggca cccacggtga agtagcggag cgccgaggcg taggccgtcg aggcctgcgc
      481 gcgcctgccg gcgagcaggt ggagctcggc gagccgctcg cgctcgggcc ccgcgtcgat
      541 caacgcgaga ccatgatcga gctggctgac gatctcgaac accttcccgg ggagctcctc
      601 gggcggcgtg ctcgcgagca tccggcggcc gatcttgagg tggacccgcg cgcgctccgc
      661 ctcgggcacg agggcgtagg cggcctcgtg cacgcggtca tggggaaacc gatagatgca
      721 ccgatcggcc tggacgctct cgaacagcaa gtgctgctcg atcgcggggc gcagcgccgg
      781 tgcgacctcg cgctcgcacg cgatcgtcag cgtggcgtca tcgaccgtgc cgccgatgca
      841 cgccgccccc tggagcgccg cctgggtctc gctcggcagc gcgtacagcc ggctacgcat
      901 cagctcggcg atgttgtcgg tatagccctc ggcgcggacc cgcgcggcat cccatcgcca
      961 gcgatacgcg tcgcgatcga accagatcga gcgcttgtgg tggagcgcgg tcaggaactg
     1021 gatcgcgaag aacgggttgc cgccggtctt gtcgcggacc aggcccgcga gcggcgccgc
     1081 ctcgtcgagc gatgcgtgga ccgtgtcggc gaccagccgg ccgaggtcct gctcggacag
     1141 cggcccgagc accaggtcgc ggatccgcgc gtggctccgc cgcgccgcct cgagcgcgcg
     1201 caccgtcggg tgagaccgat cgacctcgtt gtcgcgatag gcgccgatga tcagcaggtg
     1261 gtgggtattg ccatcggtga gcaggctggc gatcagcgcc aggctcgcgc tgtccgccca
     1321 ctgcatgtcg tcgatgaaca tcgcgagcgg gtggtcgggc gtcgcgcacg ccgcgaacag
     1381 cctgccgaac acccggcgaa gccgggtctc cgacgcggtc aagggcagct cgggcaccgg
     1441 cggctgcggc ccgacgatca gcccgagctg cgggaccagg tccacgatca gctggccatg
     1501 cggcccgagg gcctgggtga accgctgccg ccaccgcgcg atcccgagct cgccttccgc
     1561 caggatatcg agcgcgagct cgcggagcgc ctgggtgatc gtgaagtacg ggatgtcgcg
     1621 cttgtactgc tcgaacttgc ccgtgatcag ccgcccgcgc cgctggacga ccgtgcgcag
     1681 cagctcgcgc accaccgacg acttgccgat gcccgcgtag cccgacacca cgatcagctc
     1741 cggggtcccg gactcggcag cgcgctcgaa cgcctcgcgc agcgcggcgc actcggcgtc
     1801 gcggccgtac agcttctgcg ggatccggaa ctcctcggag acgtcgcgct cgccgagcgg
     1861 gaacgacagc agcgcgccgt gctccgtcca gtcgcgcagg cagcgctcca gatcgtggca
     1921 caggcccagc gcgctctggt agcgatggtc gggcagcttc gccagcagct tgcccacgat
     1981 gtcgatcagc tggcggggca ccggcggtcg cagctggtcg aagggccgcg gccggcgcgc
     2041 gacgtggcag tgcacccagc ccaccgcgtc ctcggcctcg aacggcagcc ggccggcgag
     2101 cagctggtag aacaccacgc ctagcgagta cagatccgag cggctgtcga tcggccggtt
     2161 catctgcccg gtctgctcgg gcgcgatgta cggcagcgag cccgcgctca gcgcaggggc
     2221 cctcgatccg gcgatcgggg gatccacgag cgcgagctcc ccgagctcgg catcgaacag
     2281 gacgctggac ggccggatgt cctggtggat cgcgccccgc gcgtgcagcg cggcgagctc
     2341 cccggtcagg cggatcgcca gcggcaggaa ttgctggatc ggcatccgca caccggtcca
     2401 ctgatcgagc gaggcgctcg cgcgcaccgg gggtcccgac ctcgcgccgg gctgctcaga
     2461 cgcctgcatc ctggtctctg cgaacgtgac acctgcttca gatcgtccac catgttactc
     2521 ccagcacgag gctcgcgtac atggcgatcc gatctcacgg gacccgcgcg tgaattgcgc
     2581 aggatacgga cagtcgctga caaacgatcg cgcgcgctca cggatcccgg ctcccggaaa
     2641 ttctcgcccg ggccgcgctc gttcgcgtca ttcgcgcggg gggcgcccgg gcgaccgctg
     2701 cagctcgggc cacagccagc ggtggccggc gtacaccacg aggccggcca ggatgccgat
     2761 gccgcccgcc atcgcctgcg cgcgcgacac gccggtgagc agccagatcg agagcgcgac
     2821 ggcgagcagc gggatcaccg gcccgccggg caggacgaag cgcggcggga gctcgcgccg
     2881 ccgccgcaag atcggcaccg ccaggcagat gatcgcgctg aacagcaggc gggacaccgc
     2941 gctcagcgag acgagctggg cgaacccgcc gctgaccgca gccgcccagg tcagcacggc
     3001 gaacgcgatc accgcccggt gcggcgagtg ataccgcggg tggatcgccg ccagcccggc
     3061 gggcagctga ccatggcgcg cgaacgcata gaggacgcgc ggtccgacca ggatcacggc
     3121 gctgatgctg ccgagcgtcg cgacgatcgc ggccgccgtg agcagccagc cgcccggcgc
     3181 ccccatcatc gcgcccgccg ccgaggcgag cggcgtctcg ctggtggcga gcccgggcag
     3241 cacgctctgc gccacgacct ggatcaggat gtagagcacc gcgaccgcgg cgatcgtggt
     3301 gagcagcgcg accggcaggt ggcggcgcgg gtcgaccgcc tcctcggtcg gcacgttggc
     3361 gttctcgaag ccgccgtagg cgaacaccag gggcagcgcg gcctggcgca gcgagccgct
     3421 cggcagcccg agcagcgcca cgcggccggg ctcgacgaac gcaaggccga cgccgacgaa
     3481 cacgagcagc gggatcagct tggcgatcgt caacaggttg accgtcgacg acgccgcgcg
     3541 cacgccgtac acgttgatcg cggcgaggcc gccgatcgcg accgtgatcg ccgcgaaccg
     3601 cccggggctg gtggccagcg cgggcacgac gctgcccagg taggccacga acgtgttggc
     3661 gatcgcggcg gtcgcgaccg cgcgggtgag caggaacagc cagccgacca ggaagccgac
     3721 gaagtcgccg aacgcgtcgc gggcgtacag gtacggccct ccggtctcgt cggagcggct
     3781 gccggcctcg gcgaagcatg cgaccaccag cgccatcagc acggccgcga ccaggtaggc
     3841 gatcgggctg gccgcgccgg cgagcgcgta gaccgtggcc ggcaaggaga agatcccggc
     3901 gccgatcacg ccgttgagcg cgatcgcggt cagcccggcg aggccgacca cacgcaagag
     3961 gcgcggctgg gaccccgtca cacgttgttg aggatgcgga gcgccgtgcc gctgaagatg
     4021 gattgcttct cgtgcgcggc gatgtcgagc gcctcgatcg acgtcaccgc gcgatcgatc
     4081 gagcccagca tgtgggggta gtcgctgccc atcaccatgt gatcgacgcc gaccagatcg
     4141 cgcaccatgc gcagcgccgg cccgctgaag ctcaccgtgt cgtagtgcag gcgcttgagg
     4201 tacgtgctcg gcagcgcgtc gatgtgcgcc cggcactcgg cgaagtcgcg gtatccgttg
     4261 tccaaacgct ccatcagata cggaatggca cctccgaggt gaccgatcac ccagcggatg
     4321 tcggggaagt cgcggaacat gccggcgaag cacatccggg ccaccgcgag gctggtgtcg
     4381 aacggaaagc cgaccagcgg gccgagcacg aactcgttga acacctcgga cgcgaccggg
     4441 atcatcgggt gcaggaagat gcacagccgg cggcggttgg cctcctcgaa gaacggccgg
     4501 tagctcgggt cggtgagcgc gcggccccgg atgttgctga gcaggacgac gccgctcagc
     4561 cggagctcgc cgagcgcgcg gtcgagctcc cggagcgccg cgtcgggggc gtccatcggg
     4621 atcgacgcga accccttgaa ccgcgtgggg tgcttcgcga tcaggtcggc gtaggcgtcg
     4681 ttgacgaccc tggcgacctc ggcctgcccg gctgcctcgg cgaagaacac gttgggcgtc
     4741 gacagcgaca gcacctcgac gtcgatcccg acccggtcca tgtcggccag gcgcagcgcc
     4801 ggatcggtca tcggcgcggt gacgccgaag aaccgcgcgc cgcggtggcg gatgatcgtc
     4861 cggccggtcg ggtcggtgcc gaacgagaac tcgcccggcg tgttgcggat caggtcgaag
     4921 aacgacgccg gatagtagtg ggtgtgcaga tcgtaccgca tgcggactcc tcgtcgcggt
     4981 ggcgcggcgg cagcctagca cgcggcggtc accgcctact ggtggcgcgg cacgcagttg
     5041 ctgatcagcg cgtgcgccgc gtcggacagg ccggtcccga gcccctcgcc ctggcagccg
     5101 tcggactgga agtagcagtg gtactgggcg aggttcggat tgctcccctc ggcctgcacg
     5161 atcgtgttga agtagttatt gcccatcacg gtcacagccg tcgcctggaa cgtgcacgcg
     5221 aagaactgga cctgggtctg ggggtagttg tagacgccgc cgatgtcaat cgagtcggac
     5281 ttccagcgcg cgagctcggt cgccggcacg ttgtggctgc cgcacgtcag cgggggctga
     5341 atgttctccc acgcgttgag cgaccccggc agccgcgtgg tgtcggcgac gccgcacacc
     5401 gtcgcggtcg gcggcgcgtc gccgttgcag ccgatgtcga tccgcccgaa cggcgggccc
     5461 gacagctcgt tgacgtagtc gagcacgctg cccatgccgt agtgcgccag cgcgtagccg
     5521 agctggccgg acccgccgga gaagccctcg ccgcagaacc cgaggctgcg gctgctgttg
     5581 tgcagcgtcg gctcggtgaa cacccactgc aggatcgtcg ccgggcgaca cgcggcggtc
     5641 cggatcccgg agctctgggt ctgctcccag tcgctgaccc agctgatgta gacgttgcgg
     5701 aacccggcgg cctcgtagcc ctggttgacg ccctggaagc cctcgccgcc gccgccgatg
     5761 aagtggacca cggtgccgcg cagcgtgccc atcgccgagc ggatcgcgac gacggcgtcg
     5821 atcggctcgg tcgcgatgcc cgggcagccg gtcacggtga cctgccggca ggtcgcgccg
     5881 ggcggtgcgc ccgatgggca gttcgcgagg ttgcggacgt tggtcaccgt accgagaccc
     5941 gagagggcgg ccacatcgat cgcgctggcg ggttcgtccg cggcgggatc cgacgccggg
     6001 cccgagcagt tgctgcacgc gaccagagca aggaggtaga tacttcttcg catccccccg
     6061 cgcagtgcaa gcgtcagacc acgcgaaccc tcgagaatcc ggtgagccac cggtaccgcg
     6121 aggtgccggt ccggggccgg atccgggaga cggccgtacc aggccccgtc gcgaagcgag
     6181 gtcgatctcc cggagcgatc gtgtaggttg gttcgcatga cccaccgctg gctcgcgttc
     6241 gccgcggtga cgctggccgc gccggccgcg ttcgccgacc gcgccgtgac cggctcgatg
     6301 gtcgacgacg cgaccgggtt gccggtgatc ggcgcgctgg tcaccgtcgg cagcggcgag
     6361 gcggcgaccg acgatcaggg ccagttccgg gtcgacggcg tcgcgttcgg gcgggtcgac
     6421 gtggtggtga tcgccgacgg ctaccgcgcg tacttcggct cggcgcggat cggcgcggtg
     6481 ctcgcgatcc ggctcgaggc cgccggagcc tccagcgagg tgatccgggt cagcggccgt
     6541 ccgccgggcg ggccgccgct ccacctcgac accgccgcgg tgcggaccca gccaggcgcc
     6601 ggcaacgacg tgctgcgcgc gctgcagagc ctgcccggcg tggc
//
LOCUS       DASVOR010001826         2132 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_281170,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001826 DASVOR010000000
VERSION     DASVOR010001826.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281170"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="VF516_33900"
     CDS             <1..863
                     /locus_tag="VF516_33900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112791.1"
                     /translation="GDGWQRVDQSTKKLGAEFSVTEYVRFHASATLEGTLDLAYEPKT
                     HIATLWFKPTRSPDVQFEPTGKVEVDAHGLWSSIIGGIATAIGKAPGKEARKQVEQQG
                     QQSFASKLDDGMTVTFNACSGSTRSQFGLLAPGTEAPLDVGDTTQVEVELKRGGILAF
                     GPEYATRPVSIDARASSGPVHVEAACLAEAEKLTKAYVAGEPLPQIETIAAAEVTGHR
                     QLALAKPRCPFVVIARSEGGTVSLRWKRSPPPGSALIDCPPQQANPETAPHAAATPHN
                     TPATPGPHGK"
     gene            1097..>2132
                     /locus_tag="VF516_33905"
     CDS             1097..>2132
                     /locus_tag="VF516_33905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112792.1"
                     /translation="MATVACIASLSGCGTGPGAMPPGTPPTGDPATGPGGGSNLTCNH
                     SSFPPDAALYQDISAAAVDPESSTIMAALDARSWGDSNERQTLGIDFSFEINCAAASV
                     ARLPYLQNGNNQPDCDLAAVPVPSGGKTEGASNYACVGGDCHLIVYQGSRLYELYQAN
                     ITGGAATGGTFTGTCLAIWDLTRDYWNPVNHSPDYSRGEDCDGGTAADLPIAPMLLTA
                     EELNAAIKGDGMIHHALRFTLNNNRIRSSAYVHPATHYAFGGTTGGADTLPTGARLRL
                     RGCYQIDGLPSDAARVVARTLQHYGMYLTDGGNSYISATTDAAGVMGISGSALGAIRP
                     HDFEMVGGGQR"
BASE COUNT          348 a          793 c          711 g          280 t
ORIGIN      
        1 cgggcgacgg ctggcagcgc gtggaccaga gcacgaagaa gctcggtgcc gagttctcgg
       61 tcaccgagta cgtgcggttc cacgcgtccg cgacgctcga aggcacgctc gatctcgcgt
      121 acgagccaaa gacccacatc gcgaccctgt ggttcaagcc gacccgctcg cccgacgtgc
      181 agttcgagcc gaccggcaag gtcgaggtcg acgcgcacgg cctgtggtca tcgatcatcg
      241 gcgggatcgc caccgccatc ggcaaggcgc ccgggaagga ggccaggaag caggtcgagc
      301 agcagggcca gcagtcgttc gcgtccaagc tcgatgacgg catgaccgtg acgttcaacg
      361 catgcagcgg ttccacgcgc agccagttcg ggctcctggc gccgggcacc gaggcgccgc
      421 tcgacgtcgg cgacacgacc caggtcgagg tcgagctcaa gcggggcggc atcctggcgt
      481 tcgggcccga gtacgcgacg cggccggtct cgatcgacgc gcgagcgagc tccgggccgg
      541 tccatgtcga ggcggcctgc ctggccgagg ccgaaaagct cacgaaggcg tacgtcgccg
      601 gcgagccgct gccgcagatc gagaccatcg ctgcggccga ggtcaccggc caccgccagc
      661 tcgcgctcgc gaagccgcgc tgtccattcg tggtgatcgc acggtccgag ggcggcacgg
      721 tatcgctgcg ctggaagcgc tcgccgccgc ccggctcggc gctgatcgac tgtccgccgc
      781 agcaggcgaa cccggagacc gcaccgcacg cggcggccac gccgcacaac acgccggcga
      841 cgccgggtcc gcacggcaaa tgacgttcgc gcctcgggcg ctgcattcag cccgcggcgc
      901 cggcgcacgt gtgaaccaca cctgtgactc gcctgccacg gggacctacc tggctccggc
      961 cacggcagcc gcggtctgca acgttctgcc agcgagggcc gagctccgat cgtgctaccg
     1021 taccgccagg agatagcgca tgggtgcgga ccgctcgtcg gttcgatgta cgatcatcgg
     1081 gtggaagcgt ccggccgtgg ccaccgtcgc ctgcatcgct tcgctgtccg gatgcggaac
     1141 gggtcccggc gccatgccgc ccggcactcc gccgaccggc gatcccgcga ccggcccggg
     1201 cggcggcagc aacctgacct gcaatcactc gagctttccg ccggacgcgg cgctgtatca
     1261 ggacatctcg gccgccgcgg tcgaccctga atcctcgacg atcatggccg cgctcgacgc
     1321 gcgcagctgg ggcgattcga acgagcgcca gaccctcggg atcgacttct cgttcgagat
     1381 caactgcgcg gccgcgagcg tcgcccgcct cccctaccta cagaacggca ataaccagcc
     1441 cgactgcgat ctcgcggcgg tgcccgtgcc gagcggtggc aagaccgagg gtgccagcaa
     1501 ctacgcgtgc gtcggcgggg actgccacct catcgtctac cagggcagcc ggctctacga
     1561 gctgtaccag gccaacatca ccggcggcgc ggcgaccggc ggcacgttca ccggcacctg
     1621 cctcgcgatc tgggatctca cccgcgacta ctggaacccc gtcaaccatt ctcccgacta
     1681 cagccgcggg gaggactgcg acggcggcac cgcggcggat ctaccgatcg cgccgatgct
     1741 gctcaccgcc gaggagctga acgcggcgat caagggcgac ggcatgatcc accacgcgct
     1801 gcgcttcacg ctgaacaaca accgcatccg ctcctccgcg tacgtccatc ccgcgacgca
     1861 ttatgcgttc ggcggcacca ccggcggcgc cgacacacta ccgaccggcg cgcgcctgcg
     1921 gctgcgcggt tgctaccaga tcgacggcct gcccagcgac gccgcccgcg tcgtggcgcg
     1981 cacgctgcag cactacggca tgtacctcac cgacggcggc aacagctaca tctccgcgac
     2041 gaccgacgcc gccggcgtga tgggcatcag cggctccgcc ctgggcgcga tccggccgca
     2101 cgacttcgag atggtcggcg gcggccagcg ca
//
LOCUS       DASVOR010001827         2200 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_311675,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001827 DASVOR010000000
VERSION     DASVOR010001827.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311675"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VF516_33910"
     CDS             complement(<1..692)
                     /locus_tag="VF516_33910"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEX8112793.1"
                     /translation="MTESELDFDPEELAMLRQLFRSEAQDALEAVTARVLAVGSSAPS
                     RQALTEMMRATHTMKGAAGTVGLPAMVDLAHRLENVFASFHADPGMWTREIGELLVQV
                     MDAMRGYLDIMQGPAGEAAADELRTTIDQILQPHSRVTRQMVPIVMPEAAPAIPEAVP
                     VGPETASDGVPEAVAEADAEPDAAAADVPARPEPRSYLRVEPERVDAVVSSAGELLVQ
                     VMDAMRGYLDIM"
     gene            complement(692..1111)
                     /locus_tag="VF516_33915"
     CDS             complement(692..1111)
                     /locus_tag="VF516_33915"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEX8112794.1"
                     /translation="MAELRNVIVVTIGGTRYAVELRWVREVVSLGFVTHVPTAPAALG
                     GVCNLHGTILPVLDVGVLLGGTVGLPARQGDGALVVEAEGVLCALRVDQVDHVASLPE
                     TRGAVMDASGRPMVLLEPERLVQRALELAAQGGERVA"
     gene            complement(1277..1633)
                     /locus_tag="VF516_33920"
     CDS             complement(1277..1633)
                     /locus_tag="VF516_33920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112795.1"
                     /translation="MALIVDIRHWLDEHGQLPSDNLAVRRRALRIAQLIEAGGPLAVG
                     QLRETLIPCSLRPNRKPCLGLLWVEKVEDERIYAYCLACKREEIVITGWKDTMWANGP
                     MEPASEEMFVLPANVN"
     gene            1885..>2200
                     /locus_tag="VF516_33925"
     CDS             1885..>2200
                     /locus_tag="VF516_33925"
                     /inference="COORDINATES: protein motif:HMM:NF013776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112796.1"
                     /translation="MPLPFVDRRAELGRLCRALTARTSSLVCVYGRRRVGKSRLVTMA
                     LEGLAAVYYLADERDEHLQRQSLAREIERLIPSFARVEYPDWDALLDRWAEAAPAGAV
                     LAI"
BASE COUNT          294 a          916 c          673 g          317 t
ORIGIN      
        1 tgcatgatgt ccaggtagcc gcgcatcgcg tccatcacct ggaccagcag ctcgcccgcg
       61 ctcgacacca ccgcgtcgac ccgctcgggc tcgacccgca ggtacgacct cggctccggc
      121 ctcgccggga catcggctgc cgccgcgtcc ggctccgcgt ccgcctccgc caccgcctcg
      181 ggcaccccgt ccgacgcggt ctccggcccc accggcacgg cctcggggat cgccggcgcc
      241 gcctccggca tgacgatcgg caccatctgc cgcgtcaccc ggctgtgcgg ctgcaggatc
      301 tgatcgatcg tcgtgcgcag ctcgtccgcc gcggcctcgc cggccggccc ttgcatgatg
      361 tccaggtagc cgcgcatcgc gtccatcacc tggaccagca gctccccgat ctcgcgcgtc
      421 cacatccccg gatcggcgtg gaacgacgcg aacacgttct ccagccggtg cgccaggtcg
      481 accatcgccg gcagcccgac cgtccccgcc gcgcccttca tcgtgtgggt cgcccgcatc
      541 atctcggtca gcgcctggcg cgacggcgcc gacgagccca ccgccagcac ccgcgcggtc
      601 accgcctcca gcgcgtcctg tgcctcgctg cgaaacagct ggcgcagcat cgccagctcc
      661 tcgggatcga agtcgagctc gctctcggtc atcatgcgac tcgctccccc ccctgggccg
      721 ccagctccag cgcgcgctgg accagccgct ccggctccag cagcaccatc ggccggcccg
      781 acgcgtccat caccgcgccg cgggtctccg gcagcgacgc cacgtggtcg acctgatcca
      841 cccgcagcgc gcacagcacc ccctcggcct ccaccaccag cgccccgtcg ccctgccgcg
      901 ccggcagccc gaccgtcccg ccgagcagca ccccgacgtc gagcaccggc aggatcgtcc
      961 cgtgcaggtt gcacaccccg ccgagcgccg ccggcgccgt cggcacgtgg gtcacgaacc
     1021 ccaggctcac cacctcgcgc acccagcgca gctcgacggc atagcgcgtc ccgccgatcg
     1081 tgacgacgat gacgttgcgc agctctgcca ccgctcgatc ataccgtgca cgccgtcacg
     1141 cgtgcgccgg cggcgccgtt tgcgtcggat tccgcggtcg gtcgccccgc cccgtgaccg
     1201 agcccgatcc cggccccctc ggcccccaaa gcgctcgggt gcgcggcgcc cgtccgccag
     1261 cgcgcccgcc gccggttcag ttcacgttcg ccggcagcac gaacatctcc tccgacgccg
     1321 gctccatcgg cccgttcgcc cacatcgtgt ccttccagcc ggtgatcacg atctcctcgc
     1381 gcttgcacgc caggcagtag gcgtagatcc gctcgtcctc gaccttctcg acccacagca
     1441 gccccaggca cggcttgcgg ttgggccgca gcgagcacgg gatcagcgtc tcgcgcagct
     1501 gcccgaccgc cagcggcccg cccgcctcga tcagctgcgc gatccgcagc gcacgccggc
     1561 ggaccgccag gttgtcgctc ggaagctggc catgctcgtc cagccagtgg cggatgtcga
     1621 cgatgagagc catatccccc gtcgtatcac gatcggcccg gccatggatc cccgccaccc
     1681 cgccgttacc ccgttgcgac ggctcacgat atccgctgca ccccatccgg aacgcctcgc
     1741 cgccgggagc gaggtttcgg ccacgctgtc tcggcaattt tctgcagcag cctgcggcag
     1801 ccacaggtgg gcgcggggga gccgatcccc ggcccatggc cagaattatg cttcggaaac
     1861 ataatgtcgc ggtagcataa tgttgtgccg ctccccttcg tcgaccgccg cgccgagctg
     1921 ggtcgactgt gccgcgcgtt gacggcccgg accagctcac tggtgtgcgt ctacggccgg
     1981 cgccgggtgg gcaagtcgcg gctcgtcacc atggcgctcg aaggtctcgc ggcggtctac
     2041 tacctggccg acgagcgaga cgagcacctc cagcgccagt cgctcgcccg cgagatcgag
     2101 cgcctgattc ccagcttcgc gcgcgtcgag taccccgact gggacgcgct gctcgatcgc
     2161 tgggccgagg ccgcgccggc aggtgccgtg ctcgccatcg
//
LOCUS       DASVOR010001828         4587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_77572,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001828 DASVOR010000000
VERSION     DASVOR010001828.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77572"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1791
                     /gene="mgtE"
                     /locus_tag="VF516_33930"
     CDS             <1..1791
                     /gene="mgtE"
                     /locus_tag="VF516_33930"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEX8112797.1"
                     /translation="AEIRAVAELRGIDTDVADLVELQKTGPIPTVTKLDDEDALSAEE
                     LRDAWPLLDLEERSDGLRVLPRDEAEDFFIALSASDQAALLLHFRSGQRRQWLRLLEP
                     DDAADLIQEAGEEHRDQLLALLDAPTRKEVTALLAYEEDEAGGLMSTRYARLRPNMTA
                     DEAISYLRRQARDKLETIYYAYVVAPDQHLLGVVSFRDLFAADPRKVVSDIMETDVVR
                     VTDEMDQETVSRIFAEHDLTVIPVVDMNGKMKGIVTVDDIVDVVQEEATEDAQKFAGM
                     EVLDLPYLVSGRGEMIKKRARWLTILLIGEMLTATALGFFQHELDKAIVLSLFLPLII
                     SSGGNSGSQASTLVIRAMALGEVRLADWLRVFLREIQMGLVLGAILGSVAAVRVIVWG
                     AAGAYEGSAGEHFLLIGLTVAVSLVGCVMWGTLMGAMLPFALRKLGADPASASAPLVA
                     TLVDVSGIIIYFTIASVILTGTVIRSVPDACRITPTTAVSAAFGGTFSAARASSSDRA
                     SGCDFTGPAGKVHVRAEISDRSAFDDAMASLPGVRPVSGLGEAAYFDADRLFAYKRGT
                     RVMVSATGKLDPSSIAAGEKQVMEQILETL"
     gene            1819..2766
                     /locus_tag="VF516_33935"
     CDS             1819..2766
                     /locus_tag="VF516_33935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112798.1"
                     /translation="MFATRVYHYRQPAAVILGLKDLRKQGLTPRGLLFVALDPRGETH
                     LVVPEDLDAVESIRVGDKLSLVSPLEGRFFHFDAVHRLPGDHVLWNGDRRLSDTGSAP
                     EVAVAVAAWLKGSSAKNVFLGCTPHVPGSWWTIDHRSPVTDLHSLGFLDCVVASHGIL
                     ARKIDDPRLFLLGFPTLAQNGHPGEGWTEVFTSDLGNILLVERRVLHYRIVLTCERGL
                     LEIDISHLPDLVIETARVPMRSGFGVVGRIDNGAFAVTVGTIESWGLTNMGPATLVGS
                     PIPSLLELPRMLRTMPDDVDAKPDPRRVPRPDTDPDTRS"
     gene            complement(2770..2979)
                     /locus_tag="VF516_33940"
     CDS             complement(2770..2979)
                     /locus_tag="VF516_33940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112799.1"
                     /translation="MSFDPIPQARIVRQAGQWMAPPRAPRTEDPGGVGVLRTALVYAI
                     GLWPLTAVVLLTAGLLVLTANLGAP"
     gene            3110..>4587
                     /locus_tag="VF516_33945"
     CDS             3110..>4587
                     /locus_tag="VF516_33945"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HEX8112800.1"
                     /translation="MPSLADLSLGFRHAYLDHERLTAQLQAWADAFPSLCRLTSIART
                     PEGRDVWLITIGPDPERLRPAVWVGGNIHAAELAGSSVALAIAEDVLRLHRDQSPGTG
                     ALDLPAPIAERLREVVFFIVPRISPDGAECVLRVGRSVRSVPRDVRVERGQPRWIPGD
                     IDGDGQARAMRIRDPGGELVESHEFPGLLVERTIDDDGPFYKLYPEGHIEHFDGKRIP
                     SPFFLGDNPVDLNRNFPWTWAPPHEQIGAGPYAASEPETRGIVEFATAHPEIFAWLDF
                     HTFGGVAIRPLGHGPDAKMDPEDLAVFRQLEAWMTEYTGYPTVSGYEEFLYEPDKPLR
                     GDLSDYAYNQRGAIGYVVELWDLFRRLGMARPPKFVQYYDRVSRADLVKLAWWDRDEN
                     DGRSFPPWRPFEHPQLGPVEIGGIDPRVGIWNPPLHELDELCTAQSRVFLRVAALAPR
                     LVIADVRRQPLPGGMTRVEIEVKNDGYLGSYGLPSAKKLDFN"
BASE COUNT          664 a         1710 c         1556 g          657 t
ORIGIN      
        1 gccgagatcc gcgcggtcgc cgagctgcgc ggcatcgaca ccgacgtcgc cgacctcgtc
       61 gagctccaga agaccgggcc gatccccacg gtcaccaagc tcgacgacga ggatgcgctg
      121 tcggccgagg agctgcgcga cgcctggccg ctgctcgacc tcgaggagcg cagcgacggc
      181 ctgcgcgtgt tgccgcgcga cgaggccgag gacttcttca tcgcactgtc cgccagcgac
      241 caggccgccc tgctgctcca cttccggtcc ggccagcgcc gccagtggct gcgcctgctc
      301 gagcccgacg acgccgccga cctgatccag gaggccggcg aggagcaccg cgaccagctg
      361 ctcgccctgc tcgacgcgcc gacccgcaag gaggtcaccg cgctgctcgc ctacgaggag
      421 gacgaggccg gcggcctgat gtcgacccgc tacgcccggc tgcgccccaa catgaccgcc
      481 gacgaggcga tcagctacct gcgccgccag gcccgggaca agctcgagac gatctattac
      541 gcctacgtgg tcgcgcccga ccagcacctg ctcggcgtgg tgtcgttccg cgacctgttc
      601 gccgccgatc cccggaaggt cgtgtccgac atcatggaga ccgacgtcgt gcgggtcacc
      661 gacgagatgg accaggagac cgtgtcgcgg atcttcgccg agcacgacct gaccgtgatc
      721 ccggtcgtcg acatgaacgg caagatgaag ggcatcgtga cggtcgacga catcgtcgac
      781 gtcgtccagg aggaggcgac cgaggacgcc cagaagttcg ccggcatgga agtcctcgac
      841 ctgccgtatc tggtgtcagg ccgcggcgag atgatcaaga agcgcgcccg ctggctcacg
      901 atcctcctga tcggcgagat gctcaccgcg accgcgctcg ggttcttcca gcacgagctc
      961 gacaaggcga tcgtgctgtc gctgttcctg ccgctgatca tctcgagcgg cggcaactcc
     1021 ggctcgcagg cctcgaccct cgtgatccgc gcgatggcgc tcggcgaggt ccggctcgcc
     1081 gactggctgc gcgtgttcct ccgcgagatc cagatgggcc tggtgctcgg cgcgatcctc
     1141 ggcagcgtgg ccgcggtgcg cgtgatcgtg tggggtgccg ccggcgccta cgagggctcc
     1201 gccggcgagc acttcctgct gatcggtctg accgtcgcgg tcagcctggt cggctgcgtg
     1261 atgtggggga cgctgatggg cgcgatgctg ccgttcgcgc tgcgcaagct cggcgccgac
     1321 ccggcgagcg cgtccgcgcc gctggtcgcc acgctggtcg acgtcagcgg catcatcatc
     1381 tacttcacga tcgccagcgt gatcctgacc ggcacggtga tccgctcggt ccccgacgcg
     1441 tgccggatca cgccgaccac cgcggtcagc gcggcgttcg gcggcacgtt cagcgccgcg
     1501 cgcgcctcgt cgagcgaccg cgcgagcggg tgcgacttca ccggcccggc cggcaaggtc
     1561 cacgtccgcg ccgagatctc cgaccgctcg gcgttcgacg acgcgatggc gagcttgccc
     1621 ggcgtccggc cggtcagcgg gctcggcgag gcggcgtact tcgacgccga ccggctgttc
     1681 gcgtacaagc ggggcacgcg ggtcatggtc agcgcgaccg gcaagctcga tccctccagc
     1741 atcgcggccg gcgagaaaca ggtgatggag cagatcctgg agacgctgta gcggcgcggc
     1801 cggcgctgcg aggctgccat gttcgcgacc cgcgtctacc actaccggca gccggcggcg
     1861 gtgatcctcg ggctcaagga cctgcgcaag caggggctga ccccgcgggg cctgctgttc
     1921 gtcgcgctcg acccgcgcgg cgagacccac ctcgtcgtgc ccgaggacct cgacgcggtc
     1981 gagagcatcc gggtcggcga caagctgtcc ctggtgtcgc cgctcgaggg ccggttcttc
     2041 cacttcgacg cggtccaccg gctgcccggc gatcacgtgc tgtggaacgg cgatcgccgg
     2101 ctgagcgaca ccggcagcgc gcccgaggtc gcggtcgcgg tcgcggcctg gctcaagggc
     2161 agctcggcca agaacgtgtt cctcggctgc accccgcacg tcccgggctc gtggtggacg
     2221 atcgaccacc gctcgccggt caccgacctg cacagcctgg gcttcctcga ctgcgtggtc
     2281 gcctcgcacg gcatcctcgc ccgcaagatc gacgacccgc ggctgttcct gctcggtttc
     2341 cccacgctcg cccagaacgg ccaccccggc gagggctgga ccgaggtgtt caccagcgac
     2401 ctcggcaaca tcctgctggt cgagcgccgc gtgctgcact accgcatcgt gctgacctgc
     2461 gagcgcggac tgctcgagat cgacatcagc cacctccccg acctggtgat cgagaccgcc
     2521 cgggtgccga tgcgctcggg gttcggcgtc gtcggccgga tcgacaacgg cgcgttcgcc
     2581 gtcacggtcg gcacgatcga gtcctggggc ctcaccaaca tggggccggc gaccctggtc
     2641 ggctcgccga tccccagcct gctggagctg ccgaggatgc tgcgcaccat gcccgatgac
     2701 gtcgacgcga aacccgatcc acggcgggtg ccgcggccgg acaccgaccc cgacacccgc
     2761 tcgtagccgt cagggcgcgc cgaggttcgc ggtcagcacg agcagccccg ccgtcagcag
     2821 cacgacggcc gtcagcggcc acagcccgat cgcgtagacc agcgcggtgc ggagaacccc
     2881 cacgccaccc gggtcctcgg tgcgcggcgc ccgcggaggc gccatccact ggccggcctg
     2941 acgcacgatg cgagcctgcg ggatcggatc gaagctcacg atgtcatcct ccgggcaagc
     3001 acactaccga aacagacacc cggcgcggcg ccgttgtttc acgccgcgcg taacagggcg
     3061 aaaagccgat ggagtcgaaa gacttggccg ttgtaaggtg ccgcgcgcca tgccgtcgct
     3121 cgccgatctg tcgctggggt tccgccacgc ctacctcgat cacgagcgcc tgaccgccca
     3181 gctccaggcc tgggccgacg cgttcccctc gctgtgccgg ctgacgtcga tcgcccggac
     3241 ccccgagggc cgcgacgtct ggctgatcac gatcggcccc gaccccgagc ggctgcggcc
     3301 ggcggtgtgg gtcggcggca acatccacgc cgccgagctc gccggctcca gcgtcgccct
     3361 ggcgatcgcc gaggacgtcc tgcggctcca ccgggaccag agcccgggca ccggcgcgct
     3421 cgacctgccg gcgccgatcg ccgagcggct gcgcgaggtc gtgttcttca tcgtgcctcg
     3481 gatctcgccc gacggcgccg agtgcgtgct gcgggtcggc cggtcggtgc gctcggtgcc
     3541 gcgcgacgtc cgcgtcgagc gcggccagcc gcgctggatc ccgggcgaca tcgacggcga
     3601 tggccaggcc cgcgcgatgc ggatccgcga tccgggcggc gagctggtcg agtcgcacga
     3661 gttccccggc ctgctggtcg agcgcacgat cgacgacgac ggcccgttct acaagctcta
     3721 ccccgagggc cacatcgagc acttcgacgg caagcgcatc ccgtcgccgt tcttcctcgg
     3781 cgacaacccg gtcgacctca accgcaactt cccgtggacc tgggcgccgc cgcacgagca
     3841 gatcggcgcc gggccgtacg ccgccagcga gcccgagacc cgcggcatcg tcgagtttgc
     3901 caccgcccac cccgagatct tcgcatggct cgacttccac accttcggcg gcgtggcgat
     3961 ccggccgctc ggccacggcc ccgacgccaa gatggacccg gaggacctcg cggtgttccg
     4021 ccagctcgag gcctggatga ccgagtacac cggctatccc acggtgtccg gctacgagga
     4081 attcctctac gagccggaca agccgctgcg cggcgacctg tcggattacg cgtacaacca
     4141 gcgcggcgcg atcggctacg tcgtcgagct gtgggacctg ttccggcgcc tcggcatggc
     4201 ccggccgccc aagttcgtgc agtactacga ccgcgtctcg cgcgccgacc tggtcaagct
     4261 cgcgtggtgg gaccgcgacg agaacgacgg ccgctcgttc ccgccgtggc gcccgttcga
     4321 gcacccccag ctcggcccgg tcgagatcgg cggcatcgac ccccgggtcg ggatctggaa
     4381 cccgccgctg cacgagctcg acgagctgtg caccgcgcag agccgcgtgt tcctccgggt
     4441 cgccgcgctc gcgccgcggc tggtcatcgc cgacgtccgg cgccagcccc tgcccggcgg
     4501 aatgacccgg gtcgagatcg aggtcaagaa cgacggctac ctcggcagct acggcctgcc
     4561 gtctgcgaaa aaactcgact tcaacga
//
LOCUS       DASVOR010001829         2557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_20307,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001829 DASVOR010000000
VERSION     DASVOR010001829.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20307"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..921)
                     /gene="accD"
                     /locus_tag="VF516_33950"
     CDS             complement(19..921)
                     /gene="accD"
                     /locus_tag="VF516_33950"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEX8112801.1"
                     /translation="MAWWSRTQGLAAEPKKSVPKGIWTKCERCSTTLYEADLDANFRV
                     CTNCQHHFRMSTERRIQVVVDDGKWQEHDRDLESSDPLGFRVDGKRYIDQVKATTRKI
                     GAGDAYRAGTVAVDGIAVEAGFFAFEFMGGSMGSVVGEKITRQFERALAYRRPAVMFC
                     ASGGARMQEGVLSLMQMAKTSAARCRLREAKVPYISVLLHPTTGGVAASVAMLGDVII
                     AEPDALIGFAGPRVIEQTIRQQLPPGFQRSEFLLEHGMVDMVVPRKDLKATLARTLRW
                     FERGRAPERRTGVMRDEVEIPATD"
     gene            1094..1414
                     /locus_tag="VF516_33955"
     CDS             1094..1414
                     /locus_tag="VF516_33955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112802.1"
                     /translation="MRSPMSSVLIVNEISTEREEMTRALEAEGFTVVQAASAAEAVRE
                     IWGGSFLVAIIPTLLTGTTSTSLQQQLVQMAPEIETLIHGKNDELPALVRKVIQIRDG
                     EAAA"
     gene            complement(1533..2228)
                     /locus_tag="VF516_33960"
     CDS             complement(1533..2228)
                     /locus_tag="VF516_33960"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8112803.1"
                     /translation="MNDALERFARDFPAGTVLFEEGQAGHVMYIVVAGEIEIRRRVGE
                     AERVLAVLGDGEFFGEMAILSGRPRSATAVARSAARLLVIDGTTFEAMLRARPEIALR
                     LIKALAARLDATNHHVELLLLPTANHRVVQCLRHMAEEQLALAASQPLAPQLSAGTAI
                     FVPRRSADIAERVGLPVAEVDDVINRLRAARLVLLAGDAGIPGEGFVLPEVGRLPEFL
                     ELFSLEASGAVRA"
     gene            complement(2236..>2557)
                     /locus_tag="VF516_33965"
     CDS             complement(2236..>2557)
                     /locus_tag="VF516_33965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112804.1"
                     /translation="AERALELVVGIESGRPRLGEKLVTGLIDTVYRHRWSGVGPVRAV
                     RFPALIALGMSDRGDGWDVEMLVRAVKLGLTCDEVVLPADAHHAPRALGRGLFHILRH
                     ATMR"
BASE COUNT          398 a          908 c          874 g          377 t
ORIGIN      
        1 gctcggcgta cgtcatgcct agtcggtcgc cgggatctcg acctcgtcgc gcatcacgcc
       61 ggtccggcgc tccggggcgc gaccgcgctc gaaccagcgc agcgtgcggg ccagcgtcgc
      121 cttgaggtcc ttgcgcggca ccaccatgtc gaccatgccg tgctcgagca ggaactccga
      181 gcgctggaag ccgggcggca gctgctggcg gatcgtctgc tcgatcacgc gcggtccggc
      241 gaacccgatc agcgcgtcgg gctcggcgat gatgacgtcg ccgagcatcg cgaccgatgc
      301 ggccacgccg ccggtggtcg ggtggagcag caccgagatg tacggcacct tggcctcgcg
      361 cagccggcag cgcgccgcgg aggtcttggc catctgcatc agcgacagca cgccctcctg
      421 catgcgggca ccgccggacg cgcagaacat gaccgccggc cggcgatatg ccagggcccg
      481 ctcgaactgc cgggtgatct tttcacctac cacggatccc atggagcccc ccatgaactc
      541 gaacgcgaag aacccggctt ccaccgcgat gccatcgacc gcgaccgtgc cggcgcggta
      601 ggcgtccccg gcgccgatct tgcgggtcgt cgccttcacc tggtcgatgt accgcttgcc
      661 atcgacccgg aatcccaggg ggtcgctgct ctccaggtcg cggtcgtgct cctgccactt
      721 gccgtcatcg acgaccacct ggatccggcg ctcggtcgac atgcggaagt ggtgctgaca
      781 gttggtgcac acccggaagt tcgcgtccag gtcggcctcg tagagcgtcg tgctgcaccg
      841 ctcacatttt gtccaaatgc ccttgggtac tgattttttg ggctcagcag ccagtccttg
      901 cgtacgtgac caccaggcca tggcgcgcta gataccagag cgtcaccccc ccgtaaagcc
      961 gcgtatgctc gcgaagcgat gaattcccga tgactttctc tcgcgaggcg agcttgaacc
     1021 tggtaagcag catggggaaa aaaagcagcc cattcagggg gcatagggca cccgtctctg
     1081 tgcgtccttg gtcgtgaggt ctccgatgtc ttcggtccta atcgtcaacg aaatcagcac
     1141 cgagcgcgaa gagatgacgc gcgcactcga agccgagggg ttcacggtgg tccaggcggc
     1201 ttcggctgcc gaggcggtcc gcgagatctg gggcggatcg ttcctggtcg ccatcattcc
     1261 gacgttgctc accgggacca cgagcacgtc gttgcagcag cagctggtcc agatggcccc
     1321 ggagatcgag acgctgattc atggcaagaa tgacgagctt ccagcgctcg tccgcaaggt
     1381 gatccagatc cgagacggcg aagccgccgc ctgacccgaa actgccacgg accggcaacc
     1441 gggtcggacc ggcaaccggc cgtctgctcg cggggttcac agcaccggac gggatcgcgg
     1501 cgggccgcgc cctgtccgca gggtgaggtt gcctaggccc gcacggcgcc ggacgcttcc
     1561 aggctgaaca gctccaggaa ctccggcagc cggccgacct cggggaggac gaagccctcg
     1621 cccgggatcc cggcgtcgcc ggccagcagt accagccgcg ccgcgcgcag ccggttgatg
     1681 acgtcgtcga cctcggccac gggtagcccg acccgctcgg cgatgtcggc cgaccggcgg
     1741 ggtacgaaga tcgccgtgcc cgcggagagc tgcggggcca gcggctggct cgccgcgagc
     1801 gcgagctgct cctcggccat gtgccgcagg cactggacca cgcggtggtt cgcggtcggc
     1861 agcagcagga gctcgacgtg gtggttggtg gcgtcgagcc gcgcggcgag cgccttgatg
     1921 agccggagcg cgatctcggg ccgcgcccgc agcatcgcct cgaacgtcgt gccgtcgatc
     1981 accagcaggc gcgcggcgct gcgggccacg gcggtcgccg agcgcggccg gccgctcagg
     2041 atcgccatct ctccgaagaa ctcgccgtcg cccagcaccg cgagcacgcg ctcggcctcg
     2101 ccgacccggc gccggatctc gatctcgccg gcgaccacga tgtacatgac gtggccggcc
     2161 tggccctcct cgaacagcac ggtgcccgcc gggaagtccc gggcgaagcg ctccagcgca
     2221 tcgttcaccg cgccatcatc gcatggttgc gtggcgcagg atgtggaaca ggccgcggcc
     2281 gagcgcgcgc ggcgcgtggt gggcgtcggc cggcagcacg acctcgtcgc aggtgagccc
     2341 gagcttgacc gcgcggacca gcatctcgac gtcccacccg tcgccgcggt cgctcatgcc
     2401 gagcgcgatc agcgccggga accggaccgc gcggaccgga ccgaccccgg accagcggtg
     2461 gcggtacacg gtgtcgatca ggccggtgac cagcttctcg cccagccgcg gccggccgga
     2521 ctcgatgccg accaccagct cgagcgcgcg ctcggcg
//
LOCUS       DASVOR010001830         8545 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_545374,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001830 DASVOR010000000
VERSION     DASVOR010001830.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8545
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545374"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1264)
                     /locus_tag="VF516_33970"
     CDS             complement(<1..1264)
                     /locus_tag="VF516_33970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112805.1"
                     /translation="MLTTPDKSFGSHASFGKYRFLAEIGFGGMSEIYLTVTQGGLAGF
                     QKLVALKLLRRDLAEDEEFRRMFLDEARLAARLNHPNVVQTYDVGEDHGRYYIAMEYL
                     EGQSYERIRRTPNSARLFPLELQIRMLSQVLCGLHYAHALADYDGTPISIVHRDVTPS
                     NVLITYDGQVKLIDFGIAKVLESVHHTQVGVLKGKARYMAPEQVAGAPIDRRADLYSV
                     GVMLWETLAGKHIWIGLSEPEVIKRSGEPPPPPTDAPPVLRQICGKAMAPDPDDRYPT
                     AEALRDDLELFLAQHITPSRERDLGAAVSELFGEERARIRQIIDAQLKSSGPGERLPA
                     FRDLRIAPSGNPFDTPVAANPYRHAAGSDPGAAVSGDARGGAPGVAPGDGPGDGPGDG
                     EDAGAHSEQLPAPAAFPPASRWRLYAIAG"
     gene            1594..3090
                     /locus_tag="VF516_33975"
     CDS             1594..3090
                     /locus_tag="VF516_33975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circularly permuted type 2 ATP-grasp protein"
                     /protein_id="HEX8112806.1"
                     /translation="MMQSQSQSQGPVTGLFGGYPALPGTYDEMFEAEGGPRAPFARIA
                     ALLARLSPEELARSQALAETALLQQGVTFSVYGDSRGTEKIFPFCLLPRLISADDFRR
                     LERGLEQRLRALGMFLDDIYGDQRILAAGVVPPEMVLGAAGYRPMLRGLKPPGGVRIH
                     IAGVDLIRDPEGTWRVLEDNLRTPSGVSYVVENRLISKRMFPRLFDAARVHRVDHYPT
                     RLAETLRSVAPAASDGGADGNMVVLTPGPYNSAYFEHSFLARTMGLELVQAPDLFVDG
                     NKVYCRTTRGPRRVDVIYRRTDEAFLDPEVFRPDSVLGVPGLMRAYAAGNVALANAPG
                     NGIADDKAVYPFVPDMIEFYLSEQPLLDQVPTYVCLFDDDRTYVLDHLDQMVVKAVDE
                     AGGYGMLMGPQASAEQLDEFRRRIAAEPRRYIAQHRVELSSCPTWIAERGAVEPRRID
                     LRPYILTSREGPWVLPGGLTRVALQRGSYVVNSSQGGGSKDTWVMKDG"
     gene            3094..4083
                     /locus_tag="VF516_33980"
     CDS             3094..4083
                     /locus_tag="VF516_33980"
                     /inference="COORDINATES: protein motif:HMM:NF016086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-E domain-containing protein"
                     /protein_id="HEX8112807.1"
                     /translation="MISRVADHCFWFGRYIERAEATARMLAASLSLALDAELPPSQVW
                     RPVIVVSGEESGFAGRVAAGDDDHPTWGDGEAVQRFMVWDDDTSVSLARSIHGMRWNA
                     RSIREVLSHEAWEAVNELHLWMRGDVAPVSYAQHRDAFYQHIRRGTQLALGLLRSTML
                     HDEPLDFVWLGVLLERVNQTARMLDVHHHAFTQMSRRHEVVETALWLSLLRALSGSEA
                     YLKRAAGRVSSEGVARFLVSEPAFPRSIAYCVHSAYSRLCDIRPPDAHDLPGGVALER
                     LRVLDTWVAARRSEPLAGTGVHDVLTHVVDEVHAICDTLGRELLGAPTGSPGT"
     gene            complement(4061..4345)
                     /locus_tag="VF516_33985"
     CDS             complement(4061..4345)
                     /locus_tag="VF516_33985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112808.1"
                     /translation="MPEVPGLVIEDSGWRRSKGIAERVVTQEGPCGTNILGGPELHEL
                     AEQALRAQERLGSRLPPDVPLRQLGEDFVVHIPILSSGITVTHLKYRVIR"
     gene            4399..5055
                     /locus_tag="VF516_33990"
     CDS             4399..5055
                     /locus_tag="VF516_33990"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX8112809.1"
                     /translation="MTFSPRLDALPSPQRALWPELAAVPRRYVLYGGTALALRLAHRP
                     SVDFDFFAHDHLDHRELETAVPFVRDAAVLDQGPDTRTILVTSTGGNVKISFFGGIAF
                     GRVGDPEVTDDEVLRAASLLDLAGTKVKALLQRVEAKDYRDVAALLEHGVKLEDILGS
                     ARALFGQTFNPLIAQKTLCYFKGGDLDLLASDVRSRLLAAAGHDVVPSTLSLRSPRLD
                     "
     gene            5310..6149
                     /locus_tag="VF516_33995"
     CDS             5310..6149
                     /locus_tag="VF516_33995"
                     /inference="COORDINATES: protein motif:HMM:NF003969.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydro-lyase"
                     /protein_id="HEX8112810.1"
                     /translation="MELIMSGVEARRPSSSPAQPSPGAAAQLRHRIRDGGGCGPTSGQ
                     AVGYVQANLVILPANLAGDFLQFCQRNPKPCPLLAVSEPGDPRMPALGADLDLRTDVP
                     RYRIWRDGVLVAEPSDIVDLWRDDLVGFALGCSFSFEEALVDAGLELRHWTQNCNVPM
                     YKTDRPTAAAGVFRGPLVVSMRPFRAIDAIKAIQVTSRYAAVHGAPVHLGDPRQIGIS
                     NLSRPDFGDPVEVHADELPVFWACGVTPQAVVMEAKPAFCITHAPGHMLVTDLRNAAL
                     SLS"
     gene            complement(6255..6824)
                     /locus_tag="VF516_34000"
     CDS             complement(6255..6824)
                     /locus_tag="VF516_34000"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HEX8112811.1"
                     /translation="MASYVASAMPKKSKSAPRRIVSSAHLADNAIGGLSELEFALSMV
                     VNAYHRWMVRCIEASIRYTPGIGRAALGALDVLVLHHVNHDDRAKRLVDIVLVLNVDD
                     IHTVNYALKKLVRVGLIEGERHGKEVFYSTTARGHGLCDQYRKFRERCLIDSLTHLGN
                     PDSAISDAASLLRGLSGVYDQAARSAASL"
     gene            6869..>8545
                     /locus_tag="VF516_34005"
     CDS             6869..>8545
                     /locus_tag="VF516_34005"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEX8112812.1"
                     /translation="MTGWQFWIDRGGTFTDVVARAPDGRLHTHKLLSDSPEHYRDAAV
                     EGIRRLLGLGPGEPITPDRVACVKLGTTVATNALLERRGERTALVITRGFRDALRIGT
                     QARPRIFDRRIVLPELVYERVVEADERIGAHGELVTALDQDALRDALRAVRDDGIAAC
                     AIAFAHGYRYPTHERAAAGIARELGFAQISVSHEVSPLVKLVPRGHTTVVDAYLSPVL
                     RRYVAQIEAEMPGVPLYFMQSSGGLARARQFHGKDAILSGPAGGVVGMVRTAVASGHH
                     QVIGFDMGGTSTDVSHYAGELERAFDTEVAGVRLRAPMMSIHSIAAGGGSIIAFQDGR
                     LRVGPASAGANPGPACYGRGGPLTTTDANVLLGRIQPAWFPRVFGPRGDAPLDPEVVR
                     VRFDALARDIALATGRPTRPAEIAAGALHIAVASMAAAIKRISVARGHDVTRYTLQCF
                     GGAGGQHACAVADALGMRSVVIHPLAGVLSAFGIGLADQTAMREAALDHKLDAEGVAA
                     ARALAAELAGLAERALLDQGAEPSRLDTVRRVHVRYAGTDTALGCALPSGD"
BASE COUNT         1245 a         3068 c         2881 g         1351 t
ORIGIN      
        1 cgccggcgat cgcgtacagc cgccaccggc tcgccggagg aaacgcggcc ggcgccggca
       61 gctgctcgct gtgcgccccg gcgtcttccc catctcctgg cccatctcct ggcccatctc
      121 ctggcgcaac tcccggcgcg cccccgcgtg cgtcaccgga caccgcggcg ccgggatcgc
      181 tgccggccgc gtgccggtac ggattcgccg ccaccggcgt gtcgaacggg ttgccggacg
      241 gcgcgatccg cagatcgcga aatgcaggca gccgctcgcc cggcccgctg ctcttgagct
      301 gggcgtcaat gatctggcgg atccgcgccc gctcctcgcc gaacagctcg gagaccgcgg
      361 cgccgaggtc gcgctcgcgg ctcggcgtga tgtgctgggc gaggaacagc tcgaggtcgt
      421 cgcgcagcgc ctcggcggtc gggtagcgat cgtcggggtc cggcgccatc gccttgccgc
      481 agatctgccg cagcaccggc ggcgcgtcgg tcggcggcgg cggtggctcg ccgctgcgct
      541 tgatcacctc gggctcggac agcccgatcc agatgtgctt gccggccagg gtctcccaca
      601 gcatgacgcc gaccgagtac agatcggcgc ggcgatcgat cggcgcgccg gcgacctgct
      661 ccggcgccat gtagcgcgcc ttgcccttga gcacgccgac ctgcgtgtga tggaccgact
      721 ccaggacctt ggcaatcccg aagtcgatca gcttgacctg tccgtcatag gtaatcagga
      781 cattgctggg tgtcacgtcg cgatgcacga tcgagatcgg cgtgccgtcg tagtcggcga
      841 gcgcgtgcgc gtagtgcagt ccgcacagca cctgcgacag catgcggatc tgcagctcga
      901 gcgggaacag ccgggccgag ttcggggtcc ggcggatccg ctcgtagctc tggccctcca
      961 ggtactccat cgcgatgtag tagcggccgt ggtcctcgcc gacgtcgtag gtctggacca
     1021 cgttggggtg gttgagccgc gcggcgagcc gggcctcgtc gaggaacatc cggcgaaact
     1081 cctcgtcctc cgcgaggtcg cggcgcagca gcttgagcgc gacgagcttc tggaatccgg
     1141 ccagaccgcc ctgcgtgacg gtcagataga tctcggacat cccaccgaac ccgatctccg
     1201 cgaggaagcg gtacttcccg aacgacgcgt gcgacccgaa gctcttgtca ggcgttgtga
     1261 gcattctgcc cggccctcac cggaacgcta cttctgtaga agtatcctct gtagaacagc
     1321 cgacgctcgg cgctcaagtc aattgggttt cagctagatc cgcctctggc gagttcgcat
     1381 agaactgtaa ttctccgtcg tttcgagcac gctggtcgcg atagttcatg tgaccggcga
     1441 ggcccactcc cgcacagcga cgccgcgacg gagcgcctgc tgtcacgcgc ggtcatcgca
     1501 cggtgcggcc aggcgtcccc tgcaggtgca catctctgct gtgagcggag caccgcgacc
     1561 acagccgggg ccggttttcg ttacgatgcc ccgatgatgc agagccagtc ccagtcccag
     1621 ggaccggtca ccgggctgtt cggcggatat cccgcgctgc ccggcaccta cgacgagatg
     1681 ttcgaggccg agggcggccc gcgggcgccg ttcgcgcgga tcgcggcgct gctcgcccgg
     1741 ctatctcccg aagagctggc ccggtcgcag gcgctggccg agaccgcgct gctccagcag
     1801 ggcgtcacgt tctcggtcta cggcgactcg cgcggcaccg agaagatctt cccgttctgc
     1861 ctgctgcccc ggctgatctc cgccgacgac ttccgccggc tcgagcgcgg gctcgagcag
     1921 cgcctgcgcg cgctcggcat gttcctcgac gacatctacg gcgaccagcg gatcctcgcc
     1981 gccggcgtgg tgccgcccga gatggtgctc ggcgccgccg gctaccggcc gatgctgcgc
     2041 gggctcaagc cgccgggcgg cgtgcggatc cacatcgccg gcgtcgatct gatccgcgac
     2101 cccgaaggga cctggcgggt gctggaggac aacctgcgca ccccgtcggg cgtgtcgtac
     2161 gtcgtcgaga accgcctgat cagcaagcgg atgttcccgc gcctgttcga cgccgcgcgg
     2221 gtccaccggg tcgaccacta cccgacccgg ctcgccgaga ccctgcgctc ggtcgcgccg
     2281 gccgcgagcg acggcggcgc cgacggcaac atggtcgtgc tgaccccggg gccgtacaac
     2341 tcggcgtact tcgagcacag cttcctggcg cgcacgatgg gcctcgagct ggtccaggcc
     2401 cccgacctgt tcgtcgacgg caacaaggtc tactgccgga ccacgcgcgg cccgcggcgg
     2461 gtcgacgtga tctaccgccg caccgacgag gcgttcctcg atcccgaggt gttccggccc
     2521 gacagcgtgc tcggcgtgcc cggcctgatg cgggcctacg ccgccggcaa cgtcgcgctc
     2581 gccaacgcgc ccggcaacgg catcgccgac gacaaggcgg tgtacccgtt cgtccccgac
     2641 atgatcgagt tctacctgtc ggagcagccg ctgctcgacc aggtcccgac ctacgtgtgc
     2701 ctgttcgacg acgaccgcac gtacgtgctc gaccacctcg accagatggt cgtcaaggcg
     2761 gtcgacgagg ccggcggcta cggcatgctg atgggcccgc aggccagcgc cgagcagctc
     2821 gacgagttcc gccgccggat cgccgccgag ccgcggcgct acatcgccca gcaccgggtc
     2881 gagctgtcga gctgcccgac ctggatcgcc gagcgcggcg cggtcgagcc ccgccggatc
     2941 gacctccggc cctacatcct gacctcgcgc gagggcccgt gggtcctgcc cggcgggctg
     3001 acccgcgtcg cgctccagcg cggcagctac gtggtcaact cgtcgcaggg cggcggcagc
     3061 aaggatacct gggtcatgaa ggacggctga cccatgatct cccgcgtcgc cgatcactgc
     3121 ttctggttcg ggcgctacat cgagcgcgcc gaggccaccg cccgcatgct cgccgcctcg
     3181 ctgtcgctcg cgctcgacgc cgagctgccg ccgtcgcagg tctggcgccc cgtgatcgtc
     3241 gtgtccggcg aggagtccgg gttcgccggc cgggtcgccg ccggcgacga cgatcacccg
     3301 acatggggcg acggcgaggc cgtccagcgc ttcatggtgt gggacgacga caccagcgtg
     3361 tcgctcgcgc gctcgatcca cggcatgcgg tggaacgcgc gctcgatccg cgaggtgctc
     3421 tcgcacgagg cctgggaggc ggtcaacgag ctccacctgt ggatgcgcgg cgacgtcgcc
     3481 ccggtgagct acgcccagca ccgcgacgcg ttctaccagc acatccgccg cggcacccag
     3541 ctcgcgctcg gcctgttgcg ctcgaccatg ctgcacgacg agccactcga cttcgtgtgg
     3601 ctcggcgtcc tgctcgagcg cgtcaaccag accgcgcgca tgctcgacgt ccatcaccac
     3661 gcgttcaccc agatgtcgcg ccgccacgag gtcgtcgaga ccgcgctgtg gctgtcgctc
     3721 ttgcgcgcgc tgtcgggcag cgaggcctac ctcaagcgcg ccgccggccg ggtcagctcc
     3781 gagggcgtcg cccggttcct ggtctccgag ccggcgttcc cgcgctcgat cgcctactgc
     3841 gtccactcgg cctacagccg gctgtgcgac atccggccgc ccgacgccca cgacctgccc
     3901 ggcggcgtcg cgctcgagcg cctgcgcgtg ctcgacacct gggtcgccgc gcgccgcagc
     3961 gagccgctcg ccggcaccgg cgtccacgac gtcctgaccc acgtcgtcga cgaggtccac
     4021 gcgatctgcg acacgctcgg ccgcgagctg ctcggcgcgc ctaccggatc acccggtact
     4081 tgaggtgggt gacggtgatc ccggatgata agatcgggat gtgaacaacg aagtcctcgc
     4141 cgagctggcg gagcggtacg tctggtggca gccggctgcc gagacgctcc tgcgcccgga
     4201 gcgcctgctc tgccagctca tgcagctcgg gaccgccgag gatgttcgtg ccgcacgggc
     4261 cctcctgggt gacgacgcgt tccgcgatgc ccttcgatcg gcgccacccg gagtcctcga
     4321 tgacaagtcc tggaacttct ggcatcgcgt gctgttcggc tccccacctc ctccccagcc
     4381 atcgcggccg ctgccgccat gaccttctct ccacggctcg atgcactgcc atctccgcag
     4441 cgcgcactct ggccggagct ggcggcggtc cctcgccgct acgttctcta cggtggtacg
     4501 gccctcgcgc tacgccttgc gcaccgaccc tcggtcgact tcgacttctt cgcgcacgat
     4561 cacctcgatc atcgcgagct ggagaccgcc gtgcccttcg tgcgcgatgc cgcggtgctc
     4621 gaccagggcc ctgacacgcg cacgatcctg gtcacgagca caggcggtaa cgtcaagatc
     4681 tcgttcttcg gtgggatcgc gtttggtcgc gtcggagatc cagaggtcac tgacgatgaa
     4741 gtcctgcgcg cggcatcgct gctcgatctg gccggcacca aggtgaaagc gctgctccag
     4801 cgcgtcgagg cgaaggacta tcgtgacgta gcggcgctcc tggagcacgg cgtgaagctc
     4861 gaggacatcc tggggagcgc tcgcgcgctg tttggtcaga ccttcaaccc gctcattgcc
     4921 cagaagacgc tttgctattt caagggcggt gatctggatc tgctggcttc tgatgtacga
     4981 agccgcctac tggctgccgc tggacatgac gtcgtgccgt cgacattgag cctgcgctct
     5041 ccgcgcctcg attgactgcg cgagcacggc aagggcgagg agcgggtgac gttgatggcc
     5101 ctgggtgggc gcggccctac ttcggcttct gcgcaggggc ccgggccaac tcgaggacgc
     5161 ccttgcgcag gccgatctcg gggacgtcgc cgttgatcgt gacggtgcac actggattcc
     5221 atacgacgat cacgacgatg actcgtgaac aaactatgtc ccacaggagc cacattgact
     5281 agcactcagc tcgttgtagt ctacctgcca tggaactcat catgtcggga gtcgaggcgc
     5341 gtcgtccgtc atcatcacca gcacagccga gccctggagc cgcggcccag ctgcggcatc
     5401 ggatccgcga tggcggtggg tgcggaccga ccagcggtca ggcggtcggc tacgtgcagg
     5461 ccaacctcgt gatcctgccg gcaaacctgg ccggcgactt cttgcagttc tgccagcgca
     5521 accccaagcc gtgtccgcta ctcgcggtgt ccgagcccgg cgatccccgg atgccggcgc
     5581 tgggcgccga tctcgacctc cgcaccgatg ttccgcggta ccggatctgg cgcgatggtg
     5641 tgctggtcgc tgagccaagc gacatcgtgg acctgtggcg cgatgacctg gtcggcttcg
     5701 cgctcggctg ctcgttctcg ttcgaggagg cgctggtcga cgccgggctc gaactgcggc
     5761 attggacgca gaactgtaac gtaccgatgt acaaaaccga ccggcccacc gccgccgcgg
     5821 gcgtgttccg cggcccgctc gtggtgtcga tgcgaccgtt ccgcgcgatc gacgcgatca
     5881 aggcgatcca ggtgacctcg cgctacgccg ccgtgcacgg ggcgccggtc cacctcggcg
     5941 atccgcgaca gatcgggatc tccaatctat cccggccgga cttcggcgat cccgtcgaag
     6001 tccacgccga cgaactgccc gtgttctggg cctgcggggt gacgccgcag gcggtcgtga
     6061 tggaggcgaa gcccgcgttc tgcatcaccc acgcgccagg ccacatgctg gtgaccgacc
     6121 tcaggaacgc ggcgctgtcg ctcagctgac ccgccgcaaa tcgcggcgac gcccgaccac
     6181 gccagagcgt caatcaaaat acgttgacga tttgtcaacg tttcgctagt atcacagccg
     6241 tgatcgtcag gcggctacag cgatgcagca gaccgagcgg cctgatcata gactcccgac
     6301 agcccgcgca acagcgacgc agcgtcgctg atcgcggagt cggggttgcc caggtgggtc
     6361 agcgagtcga tcaggcatcg ctcacgaaat ttccggtatt gatcgcacag accgtgcccc
     6421 cgggccgtcg tcgaatagaa cacctccttg ccgtgccgct cgccttcgat caacccgacg
     6481 cggaccagct tcttcagcgc gtagttcacg gtgtggatat catcgacgtt gagcaccagg
     6541 acgatgtcca ccaaccgctt ggcccggtcg tcgtggttga cgtggtggag gacgagcacg
     6601 tcgagcgcgc ccagagctgc acgcccgata ccgggcgtgt agcgaatcga cgcctcgatg
     6661 cagcgtacca tccagcgatg ataggcattg accaccatcg acagcgcgaa ctcgagctcg
     6721 gacagtccgc cgatggcatt gtcggcgaga tgggcagagg acacaatgcg acgaggcgcc
     6781 gacttcgatt tcttgggcat ggcagacgcg acgtaggatg ccatgccgcg cagcctgcga
     6841 accagccccc caacccctgg agccgcgcat gaccggctgg cagttctgga tcgatcgcgg
     6901 cggcacgttc accgatgtgg tggcgcgcgc ccccgacggc cggctgcaca cccacaagct
     6961 gttgtcggat agcccggagc actatcgcga cgctgcggtc gagggcatcc gacgactgct
     7021 cgggctgggc cccggcgagc cgatcacacc cgaccgggtc gcgtgcgtga agctgggcac
     7081 gacggtggcg accaacgcgc tgctcgagcg ccgcggcgag cgcactgccc tggtgatcac
     7141 ccgagggttt cgcgacgcgc tgcggatcgg cacccaggcg cggccccgga tcttcgaccg
     7201 ccggatcgtg ctacccgagc tggtctacga gcgcgtggtc gaggcggacg agcggatcgg
     7261 cgcgcatggc gagctggtga ccgcgctcga ccaggacgcg ctgcgcgacg cactgcgcgc
     7321 ggtccgcgac gacggcatcg cggcgtgtgc gatcgcgttc gcccacggtt accgctatcc
     7381 cacccacgag cgagccgcgg cgggcatcgc ccgcgagctc gggttcgcac agatctcggt
     7441 gtcgcacgag gtcagcccgc tcgtgaagct ggtgccgcgc ggccacacca ccgtggtcga
     7501 cgcctacctc agcccggtcc tgcgccgcta cgtggcgcag atcgaggccg agatgccggg
     7561 cgtcccgctg tacttcatgc agagctcggg cgggctggcg cgcgcgcgcc agttccacgg
     7621 caaggacgcg atcctgtcag gacccgcggg cggggtggtc ggcatggtgc gcaccgcggt
     7681 cgcgtccggg caccaccagg tgatcggctt cgatatgggc gggacctcga ccgacgtctc
     7741 gcactacgcc ggcgagctcg agcgcgcgtt cgacaccgag gtcgcgggtg tccggctgcg
     7801 cgcgccgatg atgagcatcc acagcatcgc ggccggcggc gggtcgatca tcgcgttcca
     7861 ggacggcagg ctacgggtcg gccccgcctc ggccggcgcc aaccccgggc ctgcgtgcta
     7921 cggccgcggc ggtccgctga ccacgaccga cgcgaacgtg ctgctcggcc ggatccagcc
     7981 ggcctggttc ccgcgcgtgt tcggcccacg cggagacgct ccgctcgacc ccgaggtcgt
     8041 gcgagtccgg ttcgatgcgc tggcgcgcga catcgcgctc gcgaccggac ggccgacccg
     8101 gccggccgag atcgcggccg gtgcgctcca catcgcggtg gcgagcatgg ctgccgcgat
     8161 caagcggatc tcggtagcgc gcggccacga cgtcactcgc tacacgctgc agtgcttcgg
     8221 cggcgccggc ggccagcacg cgtgcgcggt ggccgacgcg ctgggcatgc gctcggtggt
     8281 gatccatccg ctcgccggcg tgctgtcggc gttcgggatc gggctcgcgg atcagaccgc
     8341 gatgcgcgag gccgcgctcg atcacaagct cgacgccgag ggggtggcgg ccgcccgcgc
     8401 gctggccgcc gagctcgccg ggcttgccga gcgcgcgcta ctcgaccagg gcgccgagcc
     8461 gtcccgcctc gacaccgtgc gccgcgtcca cgtgcgctac gccgggaccg acacagcgct
     8521 cggctgcgcc ctgccgtccg gcgat
//
LOCUS       DASVOR010001831         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_369996,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001831 DASVOR010000000
VERSION     DASVOR010001831.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369996"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2346)
                     /locus_tag="VF516_34010"
     CDS             complement(<1..>2346)
                     /locus_tag="VF516_34010"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX8112813.1"
                     /translation="AGCATGEEAYSLAMLFCEAFAARNRPVRLKLLATDVHQASLEHA
                     GAGVYGEEQLQFVSPRRLERFFTKRSSGYQISHDLRQLIVFARHNVTKDAPFTKMHFI
                     SCRNLLIYFQPQPQRTVMSLFHFGLAPGGVLFLGASETPGALADEFVSIDDHWKIYRK
                     RRDVQLLSQIRMPLHRQAPRRPALIDLPRPHGPDPLIIETYDQLLDRFMPAGFLIDED
                     CALVDSFAGAEKLLKIRRRRPSSNLLDLLDDELRAVVSGAIQRAVRERAPVAYSGVRL
                     PSVDGTNGAEPRCTLTAEPLVHPRTATRHVLVTFQPTPEPHDPSHDPPRAEPMPVIQA
                     SREHLSTLETELAYTRETLQATIEELETSNEEMQATNEELIASNEELQSTNEELHSVN
                     EELYTVNAEYQQKIMELKELNTDLAHLLEGTDVGTVFLDRELRIRRFTSRIASVFRFQ
                     PYDIGRRISDFSHNIERPQLIDDIEHVRATGAVVEDEVRDRSSVPYFLRVLPYRIGRQ
                     SDDDAESAPIDGVVLTLTDLSALDRARAHVSRLSAIVESSEDAIIGNTLGGTITTWNR
                     GAERLFGYPAGEAIDRGLRMLAAGGSELELERAFDRIRRGETIEHVQWLSRHRSGAAV
                     EISTTLSPIHDRSGAIVGVSAIGRDISPQLAAQRELEQRQRRITVLLEQAEEGGRHRE
                     QFIAMLSHELRNPLAAVMNATTLIGKQPDAESVARCQGVIERQARHMKRLLDDLLDVS
                     RITRGKFQLILDDVDLRGPIEAAIESTAPLFAERGVTLEHSL"
BASE COUNT          318 a          756 c          907 g          365 t
ORIGIN      
        1 cagcgagtgc tcgagggtga cgccgcgctc ggcgaacagc ggggcggtcg actcgatcgc
       61 ggcctcgatc gggccgcgca ggtcgacgtc gtcgaggatc agctggaact tgccgcgggt
      121 gatgcgggag acgtcgagca gatcgtcgag caggcgcttc atgtggcggg cctggcgctc
      181 gatgacgccc tggcagcgcg cgaccgactc ggcgtcgggc tgcttgccga tcagggtggt
      241 ggcgttcatc accgcggcga gcgggttgcg cagctcgtgg gacagcatgg cgatgaactg
      301 ctcgcggtgg cggccgccct cctcggcctg ctcgagcagg acggtgatcc ggcgctggcg
      361 ctgctcgagc tcgcgctggg cggcgagttg cggcgagatg tcgcggccga tcgccgacac
      421 gccgacgatc gcgccgctgc ggtcgtggat cggcgacagc gtggtcgaga tctcgacggc
      481 ggcgcccgag cggtggcgcg acagccactg gacgtgctcg atggtctcgc cccgccggat
      541 ccggtcgaac gcgcgctcga gctcgagctc gctgccgccg gcggccagca tccggaggcc
      601 gcggtcgatc gcctcgccgg cggggtagcc gaacaaccgc tcggcgccgc ggttccaggt
      661 cgtgatcgtg ccgcccaggg tgttgccgat gatcgcgtcc tcggacgact cgacgatcgc
      721 ggacagccgc gagacgtggg cgcgggcgcg gtcgagcgca gacaggtcgg tcagggtcag
      781 caccacgccg tcgatcggcg ccgactcggc gtcgtcatcg gactggcggc cgatccggta
      841 gggcaggacg cgcaggaagt acggcaccga gctgcggtcg cggacctcgt cctcgaccac
      901 cgcgccggtc gcgcggacgt gctcgatgtc gtcgatcagc tgcgggcgct cgatgttgtg
      961 cgagaagtcg ctgatccggc ggccgatgtc gtagggctgg aaccggaaca cgctggcgat
     1021 ccgcgacgtg aaccgccgga tccggagctc gcggtcgagg aacacggtgc cgacgtcggt
     1081 gccctcgagg aggtgggcga ggtcggtgtt gagctccttg agctccatga tcttctgctg
     1141 gtactcggcg ttgaccgtgt agagctcctc gttgaccgag tgcagctcct cgttggtgct
     1201 ctgcagctcc tcgttggagg cgatcagctc ctcgttggtc gcctgcatct cctcgttcga
     1261 ggtctcgagc tcctcgatcg tcgcctgcag ggtctcgcgg gtgtacgcga gctcggtctc
     1321 gagcgtggac aggtgctcgc gcgacgcctg gatgaccggc atcggctcgg cgcgcggcgg
     1381 gtcgtgcgac gggtcgtggg gctcgggggt gggctggaac gtgaccagga cgtggcgggt
     1441 cgcggtgcgc gggtggacca gcggctcggc ggtcagcgtg cagcgcggct cggcgccgtt
     1501 cgtcccgtcg acgctgggca gccgcacgcc ggagtacgcg accggggcgc gctcgcggac
     1561 cgcgcgctgg atcgcgcccg acaccacggc gcggagctcg tcgtcgagca ggtcgagcag
     1621 gttgctgctc ggccgccggc gccggatctt gagcagcttc tcggcgccgg cgaagctgtc
     1681 gaccagcgcg cagtcctcgt cgatcaggaa cccggccggc atgaaccgat cgagcagctg
     1741 gtcgtaggtc tcgatgatca gcggatcggg gccgtgcggc cgcggcaggt cgatcagcgc
     1801 gggccggcgc ggcgcctggc gatgcagcgg catccggatc tgcgacagca gctggacgtc
     1861 gcgccgcttg cggtagatct tccagtggtc gtcgatcgac acgaactcgt cggcgagcgc
     1921 gccgggagtc tcgctggcgc cgaggaacag cacgccgccg ggagccagcc cgaagtggaa
     1981 cagcgacatc acggtgcgct ggggctgggg ctggaagtag atcaggaggt tgcggcacga
     2041 gatgaagtgc atcttcgtga acggcgcgtc cttggtgacg ttgtggcgcg cgaacacgat
     2101 cagctggcgg aggtcgtgcg agatctggta gccgctcgac cgcttggtga agaaccgctc
     2161 gagccggcgc gggctgacga actgcagctg ctcctcgccg tagaccccgg cgccggcgtg
     2221 ctcgagcgac gcctggtgga cgtcggtcgc gagcagcttg agccgcaccg gccggttgcg
     2281 cgcggcgaac gcctcgcaga acagcatcgc gagcgagtag gcctcctcgc cggtcgcgca
     2341 ccccgc
//
LOCUS       DASVOR010001832         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_136104,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001832 DASVOR010000000
VERSION     DASVOR010001832.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136104"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1314)
                     /locus_tag="VF516_34015"
     CDS             complement(<1..1314)
                     /locus_tag="VF516_34015"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HEX8112814.1"
                     /translation="MIADPDLAVPIAIVCLIVSALCSGTEIALFSIRRLVRGQLAQGK
                     RLTDRRILAMLERPRRVTETLLIGSEASNAVLAVLAFAVLRQRYAVWPAMWLAIAVAL
                     PLIVLVTDITANTLAGKAPLAWSRLFVLALDALRIAVLPLRLVIRAMTVVVLGPFGAA
                     ARQRPAHDLSEEEFRTLVDAGSVQGQVDARERRLIHRVFEFNDKNVGQVMTPRDRIFA
                     LSYDLPAQRLVKELAARGFSRVPIYQRSLDNIRGILNAKDLVRTAAGQPLGRPLGELL
                     HEPLFVPRTTPVKRLFLTFKQKKVHMAIVVSEYGKVLGLVTMDDLLAQIFGRLRDERA
                     VLQDTIPPGRGPRVPEARSDLATGPIAAPGARSLRGGIPHAGRRRLGAGPGRRPRAPA
                     DPPGVRVQRQEGRPGHDPARPDLRAVLRPAGPAPGQGARGARVLAG"
     gene            complement(1311..>1650)
                     /locus_tag="VF516_34020"
     CDS             complement(1311..>1650)
                     /locus_tag="VF516_34020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8112815.1"
                     /translation="RGPLAAHEPAGCGDDAPAIQLVVSDSGIGISRDQVAKIFEPFFQ
                     VDSSSTRAFGGTGLGLTLAKAYVEAHGGRIWVDTAPGQGSTFTATFPAPRRAELDGEG
                     ACRRGRGEPS"
BASE COUNT          249 a          586 c          603 g          212 t
ORIGIN      
        1 acccgcgaga acccgcgcgc cgcgagctcc ttgaccaggc gctgggccgg caggtcgtag
       61 gacagcgcga agatccggtc gcgcggggtc atgacctggc cgaccttctt gtcgttgaac
      121 tcgaacaccc ggtggatcag ccggcgctcg cgggcgtcga cctggccctg cgccgagccg
      181 gcgtcgccca gcgtgcggaa ttcctcctcg gagagatcgc gcgccgggcg cggcgatcgg
      241 cccggtcgcg aggtcgctgc gggcctcggg cacccgcggc ccgcgccccg gtgggatcgt
      301 gtcctgcagc accgcgcgct cgtcgcgcag ccggccgaag atctgggcca ggaggtcgtc
      361 catcgtgacc aggccaagga ccttgccgta ctcgctgacc acgatcgcca tgtggacctt
      421 cttctgcttg aaggtcagga acagccgctt gaccggcgtg gtgcgcggca cgaacagcgg
      481 ctcgtgcaag agctcgccga gcggccggcc gagcggctgg ccggcggcgg tccgcaccag
      541 atccttggcg ttgaggatgc cgcggatgtt gtcgagcgag cgctggtaga tcggcacccg
      601 cgagaacccg cgcgccgcga gctccttgac caggcgctgg gccggcaggt cgtaggacag
      661 cgcgaagatc cggtcgcgcg gggtcatgac ctggccgacg ttcttgtcgt tgaactcgaa
      721 cacgcggtgg atcaggcggc gctcgcgcgc gtcgacctgg ccctgcaccg agccggcgtc
      781 gaccagggta cggaactcct cctcggacag atcgtgtgcc ggccgttgcc gcgccgcggc
      841 gccgaatggc ccgagcacga cgacggtcat cgcgcggatc accagccgga gcggcagcac
      901 ggcgatccgc agcgcgtcga gcgccagcac gaacagccgc gaccacgcca gcggcgcctt
      961 gccggccagc gtgttggcgg tgatatcggt gaccaggacg atcagcggca gcgcgacggc
     1021 gatcgccagc cacatcgcgg gccacaccgc gtagcgctgg cgcagcaccg cgaacgcgag
     1081 cacggcgagc acggcgttgc tggcctcgct gccgatcagc agggtctcgg tcacccggcg
     1141 cggccgctcc agcatcgcca ggatccggcg atcggtgagc cgcttgccct gggcgagctg
     1201 gccgcgcacg agccggcgga tcgagaacag cgcgatctcg gtgcccgagc acagcgccga
     1261 cacgatcagg cacacgatcg cgatcgggac ggcgagatcc ggatcggcga tcatgacggt
     1321 tctccgcgcc cgcggcggca agcgccctcg ccatcgagct ctgccctgcg cggcgcgggg
     1381 aacgtggcgg tgaacgtcga gccctggccc ggcgcggtgt cgacccagat ccggccgcca
     1441 tgggcctcga cgtaggcctt ggcgagcgtg agccccaggc cggtgccgcc gaacgcccgg
     1501 gtcgacgacg aatcgacctg gaagaacggc tcgaagatct tggcgacctg atcgcgcgag
     1561 atcccgatcc cggagtcgga caccacgagc tggatcgccg gggcgtcgtc gccgcaccca
     1621 gccggctcgt gcgcggcgag cgggccgcgg
//
LOCUS       DASVOR010001833         4823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_487002,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001833 DASVOR010000000
VERSION     DASVOR010001833.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487002"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VF516_34025"
     CDS             complement(<1..303)
                     /locus_tag="VF516_34025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112816.1"
                     /translation="MSDSLARDLPRIAEVFGDLLGASAARRVHEAPAYASDVVDDHTP
                     FELSLAIGGGAPELRVLVETVHGDSSLAARWRAARALGERLRDRHGADLRRLDQIAD"
     gene            999..2750
                     /locus_tag="VF516_34030"
     CDS             999..2750
                     /locus_tag="VF516_34030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX8112817.1"
                     /translation="MVWSFLVGASCADLPDDSVPDAGVALHATGQPGPSGRFPAPGAT
                     TADTPIGIALDVEDGVAIPLRIRSGQRFYIQQIDLRAHVDRGVEEGVAGLACAGDFAA
                     LDWAGTRQVDESFVSVPNADGTFTRRRLFREARWMNERTRFVIEQLDAAGRRLGLPLV
                     VDTGAELRRSPDDSFFVRRLRAIQWTNDCASRSDCSHAASFLEEALVELRYASGPDPS
                     AVLDAATTRLRVTWTAHHDHPYTIPVEQVHNPTWDYGFVIDLAVATPPGPGGVYTPGQ
                     TLQVKFTLRDGAGKPLHPPGVLPTFRDYLSGQDPPGIDYWNVNEKVMTYYRRKHKEKQ
                     MVIALEGPLTATGPIRNTLDFVGGIMTSRDGAVTTATPELNGFFGEAAAVPAWSTLIG
                     LQPADSPVGDTAQFTLPATALPGTYKITMKARRSYLGQELAVAKVIEIQVGTAARIAA
                     RLDTGTCRDCHQGGADLTRIGHGLSAGDRDTCTTCHVPLPFEPEGPLYVRTHFVHSRT
                     ERLDAPLIRCASCHLDRAGIQRTSKSACLSCHKRYPADHVLRYGPVVDMYIGGTLDDS
                     FQQCSTACHTNHPRSDL"
     gene            2747..3592
                     /locus_tag="VF516_34035"
     CDS             2747..3592
                     /locus_tag="VF516_34035"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8112818.1"
                     /translation="MSELTGYEHRVQEFYDSALPCYEKIMGDRWHHPDPDALAAGLPR
                     LRGCEILEERIAGLCGLGAGDRALDFGSGIGGPTLHMARVTGARFVGVSNNERLNAQA
                     RARAARAGLAGWVTFQTLDDLGYKALPFPDGTFQVVTFFESACHVPDKPALFRELARV
                     LAPGGRLGGMDWIQRPFGMYQTDAEIARFMQPVNDAISIPAHGTVASYSGHMEDAGLR
                     VAIARDLYDGVLCWSTVQDDETPAWASYEGPEQQMFRRGEEALADARRAGVFSIALWL
                     AAKPA"
     gene            3803..>4823
                     /locus_tag="VF516_34040"
     CDS             3803..>4823
                     /locus_tag="VF516_34040"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HEX8112819.1"
                     /translation="MARSPALAADELCARLGTAAPKLVVMFASRDRDHVALNRALRER
                     LPRGTRLIGATTGGEIDRDGMHSGSVVIAALTGDLEVGLGLGRDLTRNAPAAGEAAIR
                     EAARQLGARPADLGSKKYVAMVIDDSFRFRKEELLLGTMAMNQALVAVGGGAYDTEYD
                     PAKGSAILHVDGEVATDAVALALFRTDAPWAALRSHWYVPTGQTVRITRIDETAKRAI
                     EIDGQPAARRYADLLGVRIDDLEFGKPKGFAVRPTALRVGREYFIRAPWRPLDDGSIE
                     FANMLEEGSELEIVKITDPIESTRRFFESELPSRVPSPRAALMFHCGGRKLYAEATGR
                     LPELAS"
BASE COUNT          738 a         1719 c         1617 g          749 t
ORIGIN      
        1 atcggcgatc tggtcgagcc ggcgcaggtc ggcgccgtgc cgatcgcgca gccgctcgcc
       61 gagcgcgcgc gccgcccgcc agcgcgcggc gagcgagctg tcgccgtgca cggtctcgac
      121 caggacgcgg agctccggtg cccccccgcc gatcgcgagc gacagctcga acggcgtgtg
      181 atcgtcgacg acgtccgagg catacgccgg ggcctcatgc acgcgccgcg ccgccgacgc
      241 tccgagcagg tcgccgaaca cctccgcgat ccgcggaagg tcgcgcgcca gcgaatcgct
      301 caccgtcgcg agcgccccga gctgccgcag ccccagctca cgcaacgtcg gcgatgcccc
      361 acgcgtctcg tggtgtcctg cggaaacaga cgcctccacc atgactaact ccttcggtta
      421 gttcttccac cccggaatca catgttgatt ctcggttcga agcaacgtag cagcatgttc
      481 gagaccggca agtgagctca cattcgaggg cgcccgactt cacctctcaa cattctgcat
      541 ggtgcggacc gacgacagct gcgacgtccc ggaacaccgg cccggtgtac cggtacccgg
      601 cgtcatcagc gaccttgatg tgatgttgat gtgatgtctc tgtattggat gattccagta
      661 gagcggtgtt cacgacgtca gatttcagtg gagcggtgct cacgatgtcg gatttccgtc
      721 ggaaagctcg cgtcgcacct gtaccggtag tgctgccggg ggctgcaccc ccccaaccgt
      781 atgtcagcca caatgcatga ttccgttcca gtgccgagga ccgccgactt ggtgtagcac
      841 gcacacgatg ctagttcgac ttgattccta ttccgcatag agtatcaatg tcctcggggg
      901 aggagtatct ggtggttccc cgacgcgcgc tccgtccggg cgcagcttga ccacagattg
      961 tgaggtgtcg aatcatgcac aagcgattcg cagcgtgggt ggtttggtca ttcctcgtcg
     1021 gtgcatcgtg cgccgatctt cccgacgaca gcgtccccga cgccggtgtc gcgctccacg
     1081 ccaccggcca gcctggtccg tcgggtcggt tcccggcgcc cggcgcgacg acggcggaca
     1141 caccgatcgg gatcgcgctc gacgtcgagg acggcgtcgc gatcccgctg cggatccgca
     1201 gcggacagcg gttctacatc cagcagatcg atctgcgcgc gcacgtcgac cgcggcgtcg
     1261 aggagggcgt ggccgggctc gcctgcgcgg gcgacttcgc cgcgctcgac tgggccggca
     1321 cccggcaggt cgacgaatcg ttcgtctcgg tgcccaacgc cgacggtacg ttcacccggc
     1381 ggcggctgtt ccgcgaggcg cgctggatga acgagcgcac ccgcttcgtg atcgagcagc
     1441 tcgacgccgc cggccgccgg ctcggcctgc cgctcgtcgt cgacaccggg gccgagctcc
     1501 ggcgcagccc ggacgacagc ttcttcgtgc gccggctgcg cgcgatccag tggaccaacg
     1561 actgcgcgtc gcgctccgac tgcagccacg ccgcgagctt cctggaggaa gcgctggtcg
     1621 agctgcgcta cgccagcggt cccgacccca gcgcggtgct cgacgccgcg accacccggc
     1681 tgcgcgtcac ctggaccgcc catcatgacc acccttacac gatcccggtc gagcaagtcc
     1741 acaatccgac ctgggactat ggttttgtga tcgatctcgc ggtcgcgacg ccgcccggac
     1801 ccggaggtgt atacactccc ggtcagacac tgcaggtgaa atttacactg cgcgatggcg
     1861 ccgggaagcc gcttcacccg ccgggcgtct tgccgacctt ccgagattat ctgagcggtc
     1921 aggatccgcc cgggatcgat tactggaacg tcaacgagaa ggtgatgacg tactatcgcc
     1981 gcaagcacaa ggaaaagcag atggtgatcg cgctcgaggg tccgctgacg gcgaccgggc
     2041 cgatccgcaa cacgctggac ttcgtcggcg gcatcatgac gtcccgcgat ggcgcggtga
     2101 ccaccgcgac gccggagctg aacggcttct tcggcgaggc cgcggcggtg ccggcgtgga
     2161 gcacgctgat cgggctccag cccgccgatt cgccggtcgg cgacaccgcg caattcaccc
     2221 tgcctgcaac ggctctcccc ggtacgtaca aaattacaat gaaggctcgg cggtcttacc
     2281 ttggacagga gcttgcagtc gccaaggtga tcgagatcca ggtcggtacg gccgctcgga
     2341 tcgccgcacg cctcgatacg gggacctgtc gcgactgcca tcaaggtggg gccgatctca
     2401 cgcggatcgg acatggcctg tccgcgggcg accgcgatac gtgcaccacg tgccacgtgc
     2461 cgctgccgtt cgagccggaa ggtccgctgt acgtgaggac gcacttcgtt cactcgcgca
     2521 cggagcgcct ggacgcgccg ctgatccggt gtgccagctg ccacctcgac cgcgccggca
     2581 tccagcgcac cagcaagtcg gcgtgcctgt cgtgccacaa gcgttatccg gccgatcacg
     2641 tgctgcgcta cgggccggtc gtcgacatgt acatcggcgg aacgctcgac gactcgttcc
     2701 agcagtgctc caccgcgtgc cacaccaacc atccccggag cgacctatga gcgagctgac
     2761 cggctacgag caccgcgtgc aggagttcta cgacagcgcc ctgccctgct acgagaagat
     2821 catgggcgac cgctggcacc acccggatcc ggacgcgctc gccgccgggc tgccgcggct
     2881 gcgcggctgc gagatcctgg aggagcggat cgccgggctg tgcggcctcg gggccggcga
     2941 ccgggcgctg gacttcggct ccggcatcgg cgggccgacc ctccacatgg cccgcgtcac
     3001 cggcgcccgg ttcgtcggcg tgtcgaacaa cgagcgcctg aacgcgcagg cccgggccag
     3061 ggccgcgcgc gccgggctcg ccgggtgggt gacgttccag accctcgacg acctcgggta
     3121 caaggcgctg ccgttccccg atggaacttt ccaggtggtg accttcttcg agtcggcgtg
     3181 ccacgttccg gacaagcccg ccctgttccg cgagcttgcc cgcgtgctcg cgcccggcgg
     3241 ccggctcggc ggcatggact ggatccagcg cccgttcggc atgtaccaga ccgacgccga
     3301 gatcgcccgg ttcatgcagc cggtcaacga cgcgatctcg atcccggcgc acgggacggt
     3361 ggcaagttac agcgggcaca tggaagacgc cggcttgcgg gtcgcgatcg cgcgcgatct
     3421 gtacgacggc gtgctctgct ggagtaccgt gcaggacgac gagactcccg cgtgggcgag
     3481 ctacgagggc ccggagcagc agatgttccg ccgaggtgag gaggccctcg ccgacgcgcg
     3541 ccgggccggc gtgttcagca ttgcgctgtg gctggccgcg aagcccgcct gacacgcccg
     3601 gtgtgcaatg gattggcatt tctggattgc gatgtttttc cacgccccga gtgccccccg
     3661 cggccgcggg tgatatcgtg ccgccaccgc gtcggttgct gtgcttcggt tgtggccgcc
     3721 gcactccgcc ctcgcgcgtg gcctcgccca cgcgccaagg agactcgaaa tgccaagcgt
     3781 caacatgttg cacgcgcgca cgatggcccg ctctcccgcg ctcgcggccg acgagctgtg
     3841 cgcccggctg ggaacggcgg ccccgaagct ggtcgtgatg ttcgcgtcgc gcgatcgcga
     3901 tcacgtcgcg ctcaaccgcg cgctgcgcga gcgcctgccc aggggcaccc ggctgatcgg
     3961 cgcgaccacc ggcggcgaga tcgatcgcga cggcatgcac tcgggcagcg tcgtgatcgc
     4021 cgcgctgacc ggcgacctcg aggtcggcct cggcctcggc cgcgacctca cgcgcaacgc
     4081 gccggccgcc ggcgaggcgg cgatccgcga ggccgcccgc cagctcggcg cacgcccggc
     4141 cgatctcggc tcgaagaagt acgtcgcgat ggtgatcgac gactcgttcc ggttccgcaa
     4201 ggaggagctg ctgctcggca cgatggcgat gaaccaggcg ctggtcgcgg tcggcggcgg
     4261 cgcgtacgac accgagtacg accccgccaa gggctcggcg atcctccacg tcgacggcga
     4321 ggtcgcgacc gacgcggtgg cgctcgcgct gttccgcacc gacgcgccgt gggccgcgct
     4381 gcgctcgcac tggtacgtcc cgaccggcca gaccgtgcgg atcaccagga tcgacgagac
     4441 cgccaagcgc gcgatcgaga tcgacggcca gcccgcggcc cggcgctacg ccgacctgct
     4501 cggcgtccgg atcgacgacc tcgagttcgg caagcccaag ggcttcgcgg tccggccgac
     4561 cgcgctccgg gtcggccgcg agtacttcat ccgcgcgccg tggcggccgc tcgacgacgg
     4621 ctcgatcgag ttcgccaaca tgctcgagga gggctccgag ctcgagatcg tgaagatcac
     4681 cgaccccatc gagagcacgc ggcggttctt cgagtccgag ctgccgtcgc gggtcccgtc
     4741 gccgcgcgcc gcgctgatgt tccactgcgg cggccgcaag ctgtacgccg aggccaccgg
     4801 ccgcctgccc gagctggcca gcg
//
LOCUS       DASVOR010001834         1794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_281337,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001834 DASVOR010000000
VERSION     DASVOR010001834.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281337"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /locus_tag="VF516_34045"
     CDS             complement(<1..204)
                     /locus_tag="VF516_34045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112820.1"
                     /translation="MKRMFLLAVAACSMACSGHGPVGPAWPKPATRDVDGGESLAPRA
                     AARAIAAIVEEERPAERVVADKPA"
     gene            239..718
                     /locus_tag="VF516_34050"
     CDS             239..718
                     /locus_tag="VF516_34050"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEX8112821.1"
                     /translation="MIEVGKKAPAFTLESSDGGKVKLSDHAGKIVVLYFYPRDNTPGC
                     TVEANEFNAALPAFKKLGAVVLGASKDSIASHCKFRDKFGLAFPLLSDPDGKVLEAYG
                     AWGDKVMYGKKMKGILRSTVLIGADGKVVQHWPKVSVKGHAAEVLEAIQALARSAVA"
     gene            727..1098
                     /locus_tag="VF516_34055"
     CDS             727..1098
                     /locus_tag="VF516_34055"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEX8112822.1"
                     /translation="MTFRPVRPALVRLLGGISLAVAFLLAPSLGHAQKAPAGDKPAAT
                     KPAGDAKPAGDAKPALVDLNTASQADLEALPGIGEAYAKKIIAGRPYAKKDQLVSRKI
                     IPAATYQKIKDQVIAKQPDKK"
     gene            complement(1469..>1794)
                     /locus_tag="VF516_34060"
     CDS             complement(1469..>1794)
                     /locus_tag="VF516_34060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HEX8112823.1"
                     /translation="RWPDARTVARGRDGQRIEYVGIVICRQQPGTASGVVFMTLEDET
                     GFVNLVVWQQVFARFALVLKTTSLLGVTGRLQVQEGIVHLIADQVWKPQLSRPVKEVA
                     SRDFH"
BASE COUNT          313 a          650 c          577 g          254 t
ORIGIN      
        1 ggccggcttg tcggcgacca cccgctcggc cggacgctct tcctcgacga tcgccgcgat
       61 cgcgcgcgct gcggcccgcg gcgccaggct ctcgccgccg tcgacgtccc gggtcgccgg
      121 cttcggccac gccggcccca ccggtccgtg accggaacac gccatcgagc acgcagccac
      181 cgcgagcagg aacatccgct tcattggttc cacggtagca aacgtggtac gtggcggcat
      241 gatcgaagtt ggcaagaagg ccccggcgtt caccctggag tcgtccgacg gcggcaaggt
      301 caagctctcc gaccacgccg gcaagatcgt cgtgctgtac ttctacccgc gcgacaacac
      361 gccgggctgc acggtcgagg ccaacgagtt caacgcggcg ctgcccgcgt tcaagaagct
      421 cggcgcggtc gtgctcggcg ccagcaagga ctcgatcgcc tcgcactgca agttccgcga
      481 caagttcggc ctggccttcc cgctgctcag cgaccccgac ggcaaggtgc tggaggccta
      541 cggcgcgtgg ggcgacaagg tcatgtacgg caagaagatg aagggcatcc tccggtcgac
      601 cgtgctgatc ggcgccgacg gcaaggtcgt gcagcactgg cccaaggtca gcgtcaaggg
      661 tcacgcggcc gaggtcctcg aggcgatcca ggcgctcgct cgatcggccg tggcatagta
      721 gccgccatga ccttccgccc cgttcgcccc gcgctcgtcc ggctcctcgg cggcatcagc
      781 ctcgccgtcg cgttcctcct cgcgcccagc ctcggtcacg cccagaaggc cccggccggc
      841 gacaagcccg ccgcgaccaa gcccgccggc gacgccaagc cggcaggcga cgccaagccc
      901 gccctggtcg acctcaacac cgccagccag gccgacctcg aagccctgcc cggcatcggc
      961 gaggcctacg ccaagaagat catcgccggc cggccgtacg ccaagaagga ccaactggtg
     1021 tcccgcaaga tcatcccggc cgcgacctac cagaagatca aggaccaggt gatcgccaag
     1081 cagcccgaca agaagtgacc gcggctgcat acaaagctgg gccgccgtca caagtcttcg
     1141 agatggcgtt cgtgatcgtc gacgtcgacc tgatcgttga cccagagcgt tgaggtcgac
     1201 ggcgacgttc gacgttgagc ccaccatcga cgttggacgt cgaccgatct tgtgctgcga
     1261 gcctggacag agtgcgacaa cggaggcgag gtcgaggtcg acggtggggt cagacgtcca
     1321 cgtatcgctt cgctgccgtc aagctcaacg ctcgagtcaa cgtcgacgtc atcgtcgatg
     1381 tcgagcgcca cgggagcggc accgagcgcg caacttcgtg atgaatcatc agaccgacgg
     1441 caacgcgctc tcccgctcgc cacggcccct agtggaagtc gcggctggcg acctccttca
     1501 ccgggcgcga tagctgcggc ttccagacct ggtcggcgat caggtggacg atgccctcct
     1561 ggacctgcag ccggccggtg acgccgagca gcgaggtggt cttgagcacc agggcgaacc
     1621 gcgcgaacac ctgctgccac accaccaggt tgacgaagcc ggtctcgtcc tccagcgtca
     1681 tgaacacgac gcccgaggcg gtccccggct gctggcggca gatcacgatg ccgacgtact
     1741 cgatgcgctg gccgtcgcgg ccgcgcgcca cggtgcgcgc atcgggccag cgcc
//
LOCUS       DASVOR010001835        17175 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340300,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001835 DASVOR010000000
VERSION     DASVOR010001835.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17175
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340300"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..211
                     /locus_tag="VF516_34065"
     CDS             <1..211
                     /locus_tag="VF516_34065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112824.1"
                     /translation="VTRARRDVVPPAVTIPAEIQALDRNTVLARLEILRQNAKVQYAH
                     RHLSGLSDDELRRLFAHALTLTKRE"
     gene            318..995
                     /locus_tag="VF516_34070"
     CDS             318..995
                     /locus_tag="VF516_34070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112825.1"
                     /translation="MGVTVRFCAAVLSQDGVIQWASPSLRLLGFMDVSRFPEGKPIGA
                     GALARRFVDTGRLRRDPDLVPASAWIDGVSDDTWIQEHSMPTCRPGTVLTMLWAPNEP
                     DVPRELTLPPRLVPGVRDMYLSDEAGRIAYHRAMISPCPIAPSWLDVLLGVNLRAAGS
                     RSVVCAGALTSGRVTQPRAGAGREVLRRTGARSTACGRRSGTRCGAGACARGPGRRCW
                     RCTRVRY"
     gene            complement(987..1127)
                     /locus_tag="VF516_34075"
                     /pseudo
     CDS             complement(987..1127)
                     /locus_tag="VF516_34075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014570681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
     gene            1674..2033
                     /locus_tag="VF516_34080"
     CDS             1674..2033
                     /locus_tag="VF516_34080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112826.1"
                     /translation="MAVFHTRAVWSPVLAIDRPAWFFTKAAQKARYVNCVWMGVSTPL
                     YAGDGIGRESTLRYDWEMLHRRWRYETAKLLSDLVGVPISLGAISAAEPPPVKVRRGR
                     VVPRLNYTIIPTSSRRD"
     gene            2190..3041
                     /locus_tag="VF516_34085"
     CDS             2190..3041
                     /locus_tag="VF516_34085"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase family protein"
                     /protein_id="HEX8112827.1"
                     /translation="MQIRVGYELVYDCPKATPMILVLNIHYTRMSDVIRPDHMITTPA
                     IPISSYRDSYGNWCTRIVAPPGRLRLTADALINDSGQPDIVAPWLPQHPIPELPEETL
                     LFLLGSRYCETEPLLEFAWQRFEPSMPGWPRVQAICDFVHAHIEFGYEHANPTTTAWQ
                     VYQTRRGVCRDYAHLAIALCRCTHAAQAAPRPHRPSPTLRLTLVARPPCRALAGCHGP
                     SSCAGCSPRAFSSGACGGRRTVVAFVADATLTRSLLTSLGRPAEPATFAPARVPPQAK
                     LARYDPA"
     gene            complement(3208..3543)
                     /locus_tag="VF516_34090"
     CDS             complement(3208..3543)
                     /locus_tag="VF516_34090"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HEX8112828.1"
                     /translation="MVRSKENPMRLSDENLRGLTVISADGTAIGSVAELFVSSSDWRV
                     ESIRVELRKDIADRIGADRTMFHRGSIELPVAFIQSVSDAVVLSIDVEKLREVHRLAE
                     AEAMPQPSA"
     gene            complement(3856..4575)
                     /locus_tag="VF516_34095"
     CDS             complement(3856..4575)
                     /locus_tag="VF516_34095"
                     /inference="COORDINATES: protein motif:HMM:NF037012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEX8112829.1"
                     /translation="MIHVRRSAERHHDRRRKREVWRTFDPIARTDRPSDRFGSLEAID
                     ESRLPPHADVPRRIGGDAEVITYVREGAVAYDDSTGHAGVIRAGEFQRMLTVHGVRHS
                     EANASRIEWAHVFQIRLCDSAPELQPGREQKRFSAAERRGVLCLVASSNARSGSLRLG
                     QDTCIYSALLEPGQHVVHELAPRPAAWLHVVQGEITLDDLVLLTGDGAGVTAERAVSL
                     TARESSEILLFDVTSAGARGT"
     gene            4690..6375
                     /locus_tag="VF516_34100"
     CDS             4690..6375
                     /locus_tag="VF516_34100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX8112830.1"
                     /translation="MEVDGVRADDLDHKELLELDAAGGVIRFAGQRALLLDAVAMGLL
                     RKYLVENFGLTAARTVFTQFGFAHGWRMAEAMRAEFRWESDDEWRSAGTRIHALEGLF
                     RVEPGGDGPLSKTGAMVVASYEAEQHLLHFGRSDVPVCWTICGLMSGYLSRIAGNDTY
                     VLEDGCVGKGDAACHLFARSREAWGGERADELHFFEPSRLKDCLDVSLQRVTKTLQAA
                     ERKLLRHRRALVRVARNVEEPLGIVTTSAAMRQLVELTRRVAAVDSTVLITGESGSGK
                     ERIARLLHDASTRAAGPFIAVNCGAITETLLESELFGHTRGAFTGATQDRPGLFEAAN
                     NGTLLLDEVGEVSPAMQVKLLRVLQEREIRRVGENRSRHVDVRVVAATNRDLAQGVFG
                     GAFRQDLYYRLKVVELHVPPLRERRDDVLPLARVLLADAALRMKRKVSGLVPNAADQL
                     LRYGWPGNVRELENAMERAVALARGGRVELEDLPEEIRRAPPETVASGAVRPLDEIEK
                     EYIVAALALNEGNQTRTAQQLHIGSATLYRKLKSYGLIGSARTPSSGRRRDSV"
     gene            complement(6399..6665)
                     /locus_tag="VF516_34105"
     CDS             complement(6399..6665)
                     /locus_tag="VF516_34105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112831.1"
                     /translation="MSISYGDKSVATLRSLVAAIEHQFAVVPPPEQLRAAWAEMVDVL
                     ALGPPPELRTCPTCGEIGMRAATRCMYCWSSLAPLPPSQAPGSS"
     gene            complement(6784..7038)
                     /locus_tag="VF516_34110"
     CDS             complement(6784..7038)
                     /locus_tag="VF516_34110"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8112832.1"
                     /translation="MITTTWIVLAAVAIVLVYMFGGIRVVQPTSRGLVERFGKYRRVA
                     EPGFHWIIPVAERLLLVNVTDVMMKRRTATSRATPSCSAG"
     gene            7241..7663
                     /locus_tag="VF516_34115"
     CDS             7241..7663
                     /locus_tag="VF516_34115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112833.1"
                     /translation="MLAVCYLQWPWRMTTSTIKDLPPEAKADAVIEPPGARKAQPEPA
                     VLRASSEPDLESRIRQRRAELIAKLRELRSAASLEVVQARDRLQARLPELAHIIKEGV
                     VDGWASLGDTVTHKLERWLAESERSLSSHDLPSKNGES"
     gene            7660..7959
                     /locus_tag="VF516_34120"
     CDS             7660..7959
                     /locus_tag="VF516_34120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX8112834.1"
                     /translation="MMIRPLHDRILAQRLDEQDRTSGGLFIPDNAKEKPLEATVIAVG
                     SGKLLANGTIQPIAVKAGDKILIAKYSGSEVKLVGKDHIIVREDDVLGVLEEGSQ"
     gene            7956..9557
                     /gene="groL"
                     /locus_tag="VF516_34125"
     CDS             7956..9557
                     /gene="groL"
                     /locus_tag="VF516_34125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830443.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEX8112835.1"
                     /translation="MTAKHILFDSNARAHIGAGLDALANAVKVTLGPRGRNVVIDKSW
                     GAPLVTKDGVTVAKEIELEDRFENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIYRE
                     GSKLVAAGANPMDIKRGIDAAVAVVVAELKRQSTPTRGKTEIAQVAMISANGDDTIGL
                     MIADAMDKVGKEGVITVEEAKSIETTLEVVEGMQLDRGYLSAYFVTDAERMEAVLAEP
                     YLLICEARLSSMKQLLPILEQLANQSKPLLVIAEDVDGEALATLVVNKLRGALQVCAI
                     KAPGFGDRRTAMLQDLAALTGGTAITNDLGLDLEKLTLADLGRAKRIIVDKDATTIIE
                     GLGDKAAIDARVRTLRREIEDSTADYDREKLQQRLAKLVGGVALIKVGAATEVEMKEK
                     KARVEDAMHATRAAVEEGVVAGGGVALLRCTPALATMVFHDDRRYGVNIVRRAIEEPL
                     RQIAQNSGVEGAVVASKVREGKAGYGYNAAADRYEDLIAAGVIDPTKVVRVAIQNAAS
                     VASLMLTTEAVIAEHVPASDDRALA"
     gene            complement(9549..9860)
                     /locus_tag="VF516_34130"
     CDS             complement(9549..9860)
                     /locus_tag="VF516_34130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112836.1"
                     /translation="MNIAVIVDYLRDGEPAAQQILAVRDGGRDGSGALTKLAVERLFE
                     PPQEQRDPLFELQRGRGGTIRPATRARHLAMISSRWSLTYSLSMAADQSSDPGIGVPS
                     R"
     gene            complement(9857..10141)
                     /locus_tag="VF516_34135"
     CDS             complement(9857..10141)
                     /locus_tag="VF516_34135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112837.1"
                     /translation="MLQLVLGRSRRRPREDPRTTSRDDLSSVACYEIAQHVEMPVGVM
                     RGLGSDRELVPPRVRGDGLGDRVVETVVECAKLSVVIDALRSIASSVIVL"
     gene            10133..10672
                     /locus_tag="VF516_34140"
     CDS             10133..10672
                     /locus_tag="VF516_34140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112838.1"
                     /translation="MEHGIPAFLDQLAAILRSKLLRGSDVGPAATALGGDLLRGGFTV
                     AQVVHDYGDVCQPITELAIEQQATITTEEFPEKLTTAAANRREDARSGHRAVGQAAIR
                     SPRPMDCFAYRRCGRAADAAVNGVSWTGMSGRAAVGCGGELRLEEAHRAVAHIIGCRS
                     VRVAACERVLPVSNTGPLS"
     gene            10689..11057
                     /locus_tag="VF516_34145"
     CDS             10689..11057
                     /locus_tag="VF516_34145"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112839.1"
                     /translation="MKKRILFVDDDPDILEIIEGLLRDERERWDMVFAPGALRGLEEL
                     AAGTFHVVVTDLQMPDIDGAALLEIVQRSYPSTRRVMLTSETRKVELGRALRVAHQIV
                     TKPCTAAVLREAIARGLEDP"
     gene            11331..11564
                     /locus_tag="VF516_34150"
     CDS             11331..11564
                     /locus_tag="VF516_34150"
                     /inference="COORDINATES: protein motif:HMM:NF045455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112840.1"
                     /translation="MEFTLYEAGGRAVAYCDDGRHVYAFHGAPLAYIDGDSVYAFDGE
                     HLGWWDQGWVRDHHGACAFFTEGAVASGLALPP"
     gene            complement(11825..12916)
                     /locus_tag="VF516_34155"
     CDS             complement(11825..12916)
                     /locus_tag="VF516_34155"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8112841.1"
                     /translation="MPDNQKASGRDVTDQSLGEERRKTDDELARRRADIEHDADAAVA
                     NARVKADDTVRATREAADQRSPETAEGSGRLAAERHRADDLLAREREVADRQRNIERA
                     DRHRVIAALLETERQSTDASLLSERRSADSAITARDDFLGMVSHDLRTLLAGIGLNAA
                     MLMHEAPPDNAGQRVVQRADAIQRFTTRMTRLIGDLLDVTSIEAGQLGLVVAKLDACR
                     LLTETLDAFQPVAIARGIALTVDMAGDALLARFDQERILQVLANLVGNAIKFTEREGR
                     IEIAVQRQGDEVLFSVRDTGPGIAHKHLAAIFDRFWQAPTTTRRGLGLGLYISKCIIE
                     SHGGRIWAESELGRGSVFHFTLPSASSAS"
     gene            complement(13087..13371)
                     /locus_tag="VF516_34160"
     CDS             complement(13087..13371)
                     /locus_tag="VF516_34160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112842.1"
                     /translation="MVDEPAVNLVGRTFGRLTVLAREGSTGQAPRRPLWRCTCGCELV
                     VRAASLLAGRTRSCGCLRAELARGAVAAAARAQRWAALANGIPEAQQWSV"
     gene            complement(13508..13696)
                     /locus_tag="VF516_34165"
     CDS             complement(13508..13696)
                     /locus_tag="VF516_34165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112843.1"
                     /translation="MAMAVDARPAPQPQPLPPARVVRRRRPAMQWDEIATWILIAVFV
                     VIVVVVGWHLLHLQLPHH"
     gene            complement(13700..13960)
                     /locus_tag="VF516_34170"
     CDS             complement(13700..13960)
                     /locus_tag="VF516_34170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112844.1"
                     /translation="MTETESRATQRARNLPPGDARGRSVARPGAAPKTARVAPAMPGD
                     ASGQALSLPSRDARGRFVAFPTTSAPSWYVFCADGYRIPGNT"
     gene            14863..15378
                     /locus_tag="VF516_34175"
     CDS             14863..15378
                     /locus_tag="VF516_34175"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEX8112845.1"
                     /translation="MPAPVSLPLDAVLLHPRPTVRVIGKGRRERVLPLWKETAADLRA
                     WLRVRGDTPGVVELFTNARGQPITRAGLAYVLRHHARTAAARCTTLSRKRVSPHVLRH
                     TCALTILQATGDLRKVSLWLGHADMQATMAYLRADPAEKLATVAAVMPMPGRRVRLRA
                     PDKLLASLHSR"
     gene            complement(15379..15759)
                     /locus_tag="VF516_34180"
     CDS             complement(15379..15759)
                     /locus_tag="VF516_34180"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8112846.1"
                     /translation="MTRTHLEVSTPGQDSPREWTPETAADGVEAAFAIIGGRWKLRIL
                     FHLFGGRRQRFSELERALPGISEKMLGQQLRQLEQDGIVRRIDHQEKPPRVEYALTPW
                     GQSLCPALDALLQWSTAREVPTPP"
     gene            15787..16116
                     /locus_tag="VF516_34185"
     CDS             15787..16116
                     /locus_tag="VF516_34185"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8112847.1"
                     /translation="MSRNLEPSIAQYIEGVNAFDADLAATGFAVDAVVHDEGRDHAGL
                     AAIRAWIQDANTRYSTQIAIDTLTQQGDATVVHATISGRFPGSPLPLRFSFQLHAGRI
                     QRLEIAP"
     gene            16113..16877
                     /locus_tag="VF516_34190"
     CDS             16113..16877
                     /locus_tag="VF516_34190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8112848.1"
                     /translation="MSGKRALVTGGTKGIGEAIVRALTRDGARVATTGRSPLPAGQAP
                     ALFVQADVSTARGVDEVVSWVIDQLGGVDILVNNVGGSSAPAGGFQALGDDDWQHALD
                     ANLLAAVRLDRALLPGMLQQGSGVIVHISSIQRRMPLFEATLAYAAAKAALTTYSKGL
                     ANEVGPRGIRVNSIAPGFTETSAAARLIAKLRDHEGIDEAQARQRLVDSLGGIPIGRP
                     AFPDEVAELVAFLVSARAGAIHGSEYTIDGGAVPTV"
BASE COUNT         2895 a         5733 c         5729 g         2818 t
ORIGIN      
        1 tgttacgcgc gcgcggaggg atgtggttcc ccccgccgtg acgataccgg cagagattca
       61 ggcactcgat cgcaacacgg tgcttgcgcg actggagatc ctacgtcaga acgccaaggt
      121 ccagtatgcg catcggcacc tgtcggggct gagcgacgac gagctgcgca ggctgttcgc
      181 gcatgcactt acgctgacca agagggaatg atgagctatg tggccatggg tggcaagaat
      241 ggaagcagca cgtatcacgg cgatcgccgc tcgcccgccg ctgtcgggtc ccgccggtcg
      301 ccgccgcggt tcgcctgatg ggcgtgacgg tccggttctg cgccgccgtg ctgtcccagg
      361 atggcgtgat ccagtgggcc tccccgtcgc tgcgcttgct ggggttcatg gacgtatcgc
      421 gcttccctga gggcaagccg atcggcgcgg gcgcgctcgc gcgtcggttc gtggacaccg
      481 gacgactccg ccgcgatccg gacctggtgc ccgccagcgc ctggatcgac ggcgtgagcg
      541 acgacacgtg gatccaggag cactccatgc cgacgtgccg gcccgggacg gtgctgacga
      601 tgctgtgggc gcccaacgag ccggacgtcc ctcgcgagct cacgctgcca cctcgcctgg
      661 tgccgggcgt ccgcgacatg tacttgagcg acgaagccgg gcggatcgcg taccaccggg
      721 ccatgatcag cccatgtccg atcgcgccga gctggctcga tgtgctgctc ggcgtcaacc
      781 tgcgagccgc aggtagtcgc tcggtcgtct gcgctggggc gctgacatcg ggccgggtca
      841 ctcagccacg cgcgggcgct ggccgcgagg tactgcggcg caccggcgcg cggtccacgg
      901 catgcggtcg ccgatcaggg acgcggtgcg gagctggcgc ctgcgcccgc ggcccgggcc
      961 gaagatgttg gcgatgcacc cgggtgcgtt actaagaccg cctgtagacc cttggcgctc
     1021 tgctgcacgt cgtactcgac cgcttgaccc tggatgagcg tccgaaagcc cgaactcgcg
     1081 atggccgagt aatggacgaa gacgtcgcgg ccgtcatcac ctcgaatgaa gccgtaaccc
     1141 ttggcatcgc tgaaccactt gacgatgccg cggcagcgcg cggtttcgtg acggtcctcg
     1201 cggtcgcgtg accggccacg ggcatttcga ggcggcaacg gcggctgtag tgcgcagcgc
     1261 gctccggttg cctatcagat gtgatcgaca acgagtggat gcgcgaggtc tggacccgaa
     1321 ttcgtgcgcc agacgatgat acaattccgg ttgccaccgg aacgatctgc cgcggtgcgc
     1381 gacgaccttg aatgtgtagc ggctgccgaa gaggagcgcc tcattttctg tcgtcacctg
     1441 catgtcattc aagatactcg ccacacgtta tcaaaggaga ccgcctatgc aagaccgttc
     1501 aatcgtgccg cgaagtgtca gcttctcggg agcgagagtc tcatacgctc tccagactgg
     1561 cgatcaatct tgacagcatt caaagctgcg cactgtagcg gatgtaaaca tcgatcgcct
     1621 tcacagccgt aataagtaac tctccggcgc gcgtctcggt gacgagctgg ccggtggccg
     1681 tgttccacac ccgcgcggtg tggtcccccg tgctggctat cgatcggccg gcgtggttct
     1741 tcacgaaggc cgcgcagaag gcccgttatg tcaactgcgt gtggatgggc gtttcgacgc
     1801 cactctacgc aggcgatggt atcgggcgcg aatctacact gcggtatgac tgggagatgc
     1861 tgcaccggcg ttggcgctac gagacggcca agctgctgtc agacctggtc ggggtcccga
     1921 tctcgctcgg tgcgatcagc gcggccgaac ctccgcccgt caaggttcgc cgtggccgcg
     1981 tcgtgcctcg cttgaactat accatcatcc cgacatcgtc ccgtcgggac tgatcatgtt
     2041 atttagatcc agccccttag cggcgtgtga ccggcttgtt tccagccgga gaggcggcgc
     2101 gggccggcga agccgtggac ccgaccactc gccggatcgc tcgcgctgtg acaccgtgcg
     2161 gctacgtctc ccacccaggc gaggtacaga tgcagatccg cgtcggctac gagctcgtct
     2221 acgactgtcc caaggcgaca ccgatgatcc tcgtgctcaa cattcactac acgcggatgt
     2281 cggacgtgat ccgtccggat cacatgatca ccaccccggc gatcccgatc tcgtcgtacc
     2341 gtgattccta cgggaactgg tgcacccgca tcgtcgcgcc ccccggccgg ttgcggctca
     2401 ccgccgacgc gctgatcaac gacagcggcc agccggacat cgtcgcaccc tggctcccgc
     2461 agcatccgat ccccgagctg ccggaggaga cgctgctgtt cctgctcggg agccgctact
     2521 gcgagaccga gccgctcctg gaattcgcgt ggcagcgatt cgagccgtcg atgcccggat
     2581 ggcctcgcgt ccaagcgatc tgcgacttcg tgcacgccca catcgagttc gggtacgagc
     2641 acgccaaccc gaccacgacg gcgtggcagg tgtaccagac ccggcgcggg gtgtgccgcg
     2701 actacgcgca cctcgccatc gcgctgtgcc ggtgcaccca tgctgcccag gctgctccgc
     2761 ggccacaccg cccatccccg acgctgcgcc tcaccctggt ggcccgcccg ccgtgtcgcg
     2821 ccctcgccgg ctgccatggg ccgagctcct gcgccgggtg ttcgcccagg gcgttctcca
     2881 gtggcgcctg cggtggtcgc cgcaccgtcg tggccttcgt cgccgatgcc accctcaccc
     2941 gcagcctgct taccagcctc ggccgccccg ccgagcccgc aacgtttgca cccgctcgtg
     3001 tcccacccca ggccaagctt gcccggtacg atcccgcgta gcctcgtagt ctcgcaccca
     3061 ccgaccggac accggtcggc atgtactcgt ccgcgaggcc gagtacggct cggacgcgat
     3121 cgcgatctga cgtcgccaag cgttggcagc ctcgcagctt cacactcggc gggcctccgg
     3181 gccccggcgc cctcagcgct cgccagctca cgcactgggc tgaggcatgg cctcggcctc
     3241 ggccaggcga tgcacttcgc gaagcttctc gacatcgatc gagagcacga cggcatcgct
     3301 cacggactgg atgaaggcga caggcagctc gatcgacccg cggtgaaaca tcgtccgatc
     3361 tgcaccgatg cgatccgcaa tgtccttgcg cagctcgacc cggatcgact ccacgcgcca
     3421 gtccgagctg ctgacgaaca gctcggcgac ggatccgatc gctgtgccgt cggcactgat
     3481 cacggtcagc cctcgaagat tctcatcgga tagccgcatt ggattctcct tgcttcgcac
     3541 catcgtgatc ccatgtgtcg agcgcctggc tgtggagctc atctcgatcg atggcccggc
     3601 gggacacgaa gcggcgggtg tgcgccagct tccgagccac cagcgctcgt cgcctgggcg
     3661 agagcaccac cacgacggtt ccgaacgcga ttgcgaggag gcctagattc ctaaccgtca
     3721 cgctcatcag cactgcaacg tcctggccac ctgttgcgca cgtagatcgt actgttgatg
     3781 gcgatggacc gcgccggacc tggcgtcagc ccgaccctcg gtcgtgttgg tggtcatgcc
     3841 cgtcgagctt gcaagtcatg taccgcgcgc tcccgcgctc gtgacgtcga acagcaagat
     3901 ctcggagctc tcgcgtgcgg tgagcgacac cgcgcgctcg gccgtgacgc ctgcgccatc
     3961 cccggtgagc agcaccaggt catcgagtgt gatctcgcct tgcacgacgt gcagccaggc
     4021 ggcgggcctg ggcgcgagct cgtgaacgac gtgctgccca ggctcgagca gggcggagta
     4081 aatgcaggtg tcttgaccaa ggcgcagcga tccggatcgt gcgttggacg aggcgacgag
     4141 gcagagcaca cctcggcgct ccgcggcgct gaagcgcttc tgctcgcgcc cgggctggag
     4201 ctcgggcgct gaatcgcaca gccggatctg gaacacgtgc gcccactcga tgcgcgatgc
     4261 gttggcctcg ctgtgccgca ctccgtgcac cgtgagcata cgctggaact cgcccgcgcg
     4321 gatcacgcct gcgtggccag tggagtcgtc gtaggcgacc gcgccttcgc ggacgtaggt
     4381 gatgacctcg gcgtccccgc cgatgcgacg gggaacgtcg gcgtggggag gaagccggct
     4441 ctcgtcgatg gcttcgagtg atccgaatcg atcggagggc cgatcggtgc gagcgatcgg
     4501 gtcgaaggtc cgccagacct cccgcttgcg gcgacggtca tgatggcgtt cggcggatcg
     4561 gcggacgtgg atcatcgggc gatcacgctg cagcagaacc cgtaccacgc gtttgtcccg
     4621 gtgtttcagt tacttgcgct acacacgcgg acgattcatt caaggtgagg taccctgacc
     4681 atcattttga tggaggtcga tggagtgcgc gcggatgatc tggaccacaa ggagctcctg
     4741 gagctcgacg ctgcaggcgg ggtgatccgc ttcgcgggtc agcgcgcgct gttgctcgac
     4801 gcggtcgcga tgggcctctt gcgcaagtat ctcgtcgaaa acttcgggct cacggcggcg
     4861 cgtaccgtgt tcacgcagtt cgggttcgcc cacggatggc gcatggccga ggccatgcgt
     4921 gccgagttcc ggtgggagag cgacgacgag tggcggagcg ccggcacccg catccacgcg
     4981 ctcgaggggc tgtttcgtgt cgagcccggt ggcgacgggc cgttgtcgaa gaccggagcg
     5041 atggtcgttg cgtcctacga ggctgagcag catctgctcc acttcgggcg ctccgacgtc
     5101 cccgtgtgct ggacgatctg cggcctcatg agcggctacc tgagccggat cgccggcaac
     5161 gacacgtacg tgctcgagga tggttgcgtc ggcaagggcg acgccgcctg ccatctgttc
     5221 gcgcgctcgc gggaagcatg gggcggggag cgcgccgacg agctgcactt cttcgagccg
     5281 agccgcctca aggactgcct ggatgtctcg cttcagcggg tcacgaagac cttgcaggcg
     5341 gcggagcgca agctgctccg acaccggcga gcgctggtcc gcgtggcgcg caatgtcgag
     5401 gagccccttg gcatcgtgac gacgagcgcg gcgatgcggc agctcgtgga gttgacgcgc
     5461 cgcgtggccg cggtcgactc caccgtgctc atcaccggcg agagtggatc gggcaaggag
     5521 cggatcgcac gactgctcca cgatgcatcg acgcgcgcgg ccgggccgtt catcgccgtc
     5581 aactgcgggg ccatcaccga gacgctgctc gagagcgagc tgtttggcca tacccgcggt
     5641 gcgttcacgg gcgcgacgca ggaccgaccg gggctgttcg aggctgccaa caacgggacg
     5701 ctgctgctcg acgaggtcgg cgaggtgtcg cccgcgatgc aggtcaagct gctgcgcgtg
     5761 ctccaggagc gcgagatccg gcgcgtcggc gagaaccgga gccggcatgt cgacgtgcgc
     5821 gtcgtggctg cgacgaaccg cgatctcgca caaggcgtct tcggtggtgc attccggcag
     5881 gatctctact atcggctcaa ggtcgtcgag ctccacgtgc cgccgctccg cgagcgcaga
     5941 gacgacgtcc tgccgctcgc ccgtgtgctg ctcgccgacg cggccctgcg gatgaagcgc
     6001 aaggtctcgg ggctggtccc gaatgccgcc gatcagctgc tgcgctacgg ctggccgggg
     6061 aacgtgcgcg agctcgagaa cgcgatggag cgcgcggtcg cgctcgcgcg cggaggccgc
     6121 gtagagctcg aggatctgcc ggaggagatc cgccgcgcgc ctcccgagac tgtcgccagt
     6181 ggcgccgtgc gcccgctcga cgagatcgag aaggagtaca tcgtcgcggc gctggcgctc
     6241 aacgaaggca accagacccg caccgcccag cagctccaca tcgggtccgc gaccctgtat
     6301 cgcaagctga agagctacgg cctgatcgga agcgcccgga cgccgagcag cggtcgacgt
     6361 cgcgattccg tgtgacgtgt acagacgtcg cggcgggatc agctgctccc tggcgcctgg
     6421 ctcggtggca gcggtgccag agacgaccag cagtacatgc accgcgtcgc tgcgcgcatg
     6481 ccgatctcgc cacaggtcgg gcaggtccgt agctcggggg gcggcccgag agcgagcacg
     6541 tcgaccatct cggcccacgc cgcacgtagc tgttcgggcg gtgggaccac cgcgaactgg
     6601 tgttcgatcg ccgcgaccag ggaacgcagc gtcgcgacgg acttatctcc atagctgatc
     6661 gacatcggga actccttgtt cagttcgccg ggacaccggc gatatcaccg atgacattga
     6721 cgagctccga cccggcgggc accacgacct tgctgttgtt ctgcagcgac cgctcgaccg
     6781 tgatcagccg gcggagcagc tgggcgttgc ccgtgaagta gcggtccgcc gcttcatcat
     6841 cacgtcggtg acgttgacga gcaacaagcg ctcggcgacc gggatgatcc agtggaaccc
     6901 aggctccgcg acgcgccggt acttcccgaa gcgctcgacc aggccgcggc tggtcggctg
     6961 caccacgcga atgcccccga acatgtagac gaggacgatc gcgacggcgg caagcacgat
     7021 ccaggtggtg gtgatcatgg tcgcttgcag ttgcatgcga cgggccaccg cgcggtcacc
     7081 tggctggctc gatcgcgcgc cctccgggtt caactcctcg cgcgggaatc ctctgctgcc
     7141 aagatcgccg gctcgcgcgc gcgtcgcgct ccttcgaatt gattgataag tactttcatt
     7201 gtgagcgagc aggagcccgc gcgctgacga ggagcgctgc ttgctcgcgg tttgctactt
     7261 gcagtggcca tggcgcatga cgacgagtac gatcaaagat cttcccccgg aggccaaggc
     7321 cgatgcagtg atcgaaccgc ccggcgcgcg caaggcccag cccgagcccg cagtcctgcg
     7381 cgccagctcc gagcctgatc tcgagtcgcg catcaggcag cgccgcgctg agctgatcgc
     7441 gaagctccgc gagctccggt cggcggcgag cctcgaggtg gtccaggccc gcgacagatt
     7501 gcaggcgagg ctgcctgagc tcgcgcacat catcaaggag ggcgttgtcg acggctgggc
     7561 gagcctcggt gacaccgtga cgcacaagct cgagcggtgg ctcgccgaat ccgaacgttc
     7621 actctccagc cacgacctgc cttccaagaa cggggaatca tgatgatccg tccactccac
     7681 gatcgtatcc tcgcgcaacg cctcgacgag caggaccgga cgtccggcgg cctgttcatc
     7741 ccggacaatg ccaaggagaa gccactcgag gccacggtca tcgcggtcgg cagcggcaag
     7801 ctgctcgcga acggaacgat ccagccgatc gcggtcaagg ccggcgacaa gatcctgatc
     7861 gcgaagtact ccgggtccga ggtgaagctc gtcggcaagg atcacatcat cgtgcgcgag
     7921 gatgacgtcc tcggggtcct cgaggagggt agccagtgac cgcgaagcac atcctgttcg
     7981 acagcaacgc ccgcgcgcac atcggcgcgg ggctcgacgc gctggccaac gccgtgaagg
     8041 tcacgctcgg gccgcgcggc cgcaacgtcg tgatcgataa gtcgtggggc gcgccgctgg
     8101 tcaccaagga cggcgtcacc gtcgccaagg agatcgagct cgaggaccgc ttcgagaaca
     8161 tgggcgcgca gatggtgaag gaggtcgcgt cgaagacctc ggacgtcgcg ggcgacggca
     8221 ccaccacggc gaccgtgctc gcacaggcga tctaccgcga gggcagcaag ctggtcgcgg
     8281 ctggggccaa cccgatggac atcaagcgcg gcatcgacgc ggcagtcgcg gtcgtggtcg
     8341 ccgagttgaa gcggcaatcg acgccgactc ggggcaagac cgagatcgcg caggtcgcca
     8401 tgatcagcgc gaacggcgac gatacgatcg ggctcatgat cgccgacgcg atggacaagg
     8461 tcggcaagga gggcgtgatc acggtcgagg aggcgaaatc gatcgagacg acgctcgagg
     8521 tcgtcgaagg catgcagctc gatcgcggct acctgtcggc gtacttcgtc accgacgccg
     8581 agcgcatgga agcagtgctc gccgagccat acctcctgat atgcgaagcg cgtttgtcga
     8641 gcatgaagca gctcctgccc atcctcgagc agctggccaa ccagagcaag ccgctgctgg
     8701 tgatcgccga ggatgtcgat ggcgaggcgc tcgcgacgct ggtcgtcaac aagctccgcg
     8761 gagcgctcca ggtgtgcgcc atcaaggcac cgggcttcgg cgatcggcgc accgcgatgc
     8821 tgcaggatct cgccgcattg accggtggca ccgcgatcac gaacgacctg gggctcgatc
     8881 tcgagaagct gacgctcgcg gacctcggcc gcgccaagcg gatcatcgtc gacaaggacg
     8941 cgaccacgat catcgaaggc ctcggcgata aggcggcgat cgatgcgcgc gtgaggacgc
     9001 tgcgccgtga gatcgaggac agcacggccg actacgaccg cgagaagctg cagcagcggc
     9061 tcgcgaagct ggtcggaggt gtcgcgctga tcaaggtcgg cgcggcgacc gaggtcgaga
     9121 tgaaggagaa gaaggcacgc gtcgaagacg cgatgcacgc gacgcgcgcg gccgtggagg
     9181 aaggagtcgt ggccggtggc ggcgtcgcgc tgcttcgctg cacgccggcg ctcgccacga
     9241 tggtattcca tgacgatcgc cgctacggcg tgaacatcgt ccggcgcgcg atcgaagagc
     9301 cgctccgaca gatcgcgcag aacagcggag ttgaaggcgc ggtcgtggcc agcaaggtcc
     9361 gcgagggcaa ggcgggctac gggtacaacg ccgctgccga tcgctacgag gatctcatcg
     9421 cggccggcgt catcgatccg accaaggtcg ttcgcgtcgc gatccagaac gccgcgtcgg
     9481 tcgcatcgct catgttgacc accgaggcgg tgatcgcgga acacgttcct gccagtgacg
     9541 accgagccct agcgtgatgg aacgccaatg cccgggtcgg acgattgatc tgccgccatg
     9601 ctgagcgagt acgtcaggga ccatcgagag gagatcatcg ccaggtgccg cgcccgagtg
     9661 gccgggcgga tggtcccccc ccgaccgcgc tggagctcga acaagggatc ccgctgttcc
     9721 tgcgggggct cgaacagacg ctcgactgcg agcttcgtca gcgccccgga gccgtcacgg
     9781 ccgccgtccc gtacggcgag gatctgttgc gcagcgggtt caccatcgcg caggtagtcc
     9841 acaattacgg cgatgttcac aagacgatca ccgagctggc gatcgagcgc agcgcgtcga
     9901 tcaccaccga gagtttcgcg cactcgacaa ctgtctcgac aacgcgatcg ccgaggccgt
     9961 caccgcgtac gcgcggcgga accagctcgc ggtcgctgcc gaggccgcgc atcacgccaa
    10021 ccggcatctc gacatgttgg gcaatttcat agcaagccac cgacgagaga tcatcgcgcg
    10081 atgtcgtgcg agggtcctcg cgcggtcggc gccgcgagcg accgaggacg agttggagca
    10141 cggaatccca gcgttcctcg atcagctcgc cgcgattcta agatcgaagc tgctcagggg
    10201 gtccgacgtc ggacccgctg ccacggcgct cggcggcgac ctcctacgcg gtggctttac
    10261 cgtcgcccag gtagtccacg actacggaga tgtgtgccag ccgatcaccg agctggcgat
    10321 cgagcaacag gcgacgatca ccacggagga attcccagag aagctgacga cagccgcagc
    10381 aaaccgtcga gaagatgcgc gttctggcca ccgtgcggtc gggcaagctg ccatccgttc
    10441 gccgcgtccg atggattgtt tcgcttaccg gcgctgcggg cgcgctgccg acgcggcggt
    10501 caatggtgtg tcctggactg gtatgagcgg ccgtgcagcg gtcggctgcg ggggcgagct
    10561 aaggcttgag gaggctcacc gtgcggtcgc gcatatcatc ggctgccgaa gcgttagggt
    10621 cgcggcttgc gaacgcgtat tgcctgtgtc gaatacgggg ccgctttcct gatgctagac
    10681 ggaagtacgt gaagaagcgg atcctattcg tggacgacga tccggacatt ctcgagatca
    10741 tcgaggggct cttgcgggac gagcgtgagc gctgggacat ggtgttcgcg cctggcgcgc
    10801 tgcgcggact cgaggagctg gccgcgggca cgttccacgt cgtggttacc gatctgcaaa
    10861 tgcctgacat cgacggtgcg gccttgctcg agatagtgca gcgtagctat cccagcaccc
    10921 ggcgggtcat gctcaccagc gaaacgcgga aggtcgaact cggacgagcg ttgcgggtag
    10981 cccatcagat cgttacgaag ccatgcaccg ctgcggtcct gcgggaagcg atcgcgcgcg
    11041 ggctcgaaga cccgtaaccc gtaactcgac gccgcgggtc gctgtgacgg cgctggcgag
    11101 ctcgagcggg agcacgccga cgaccttggc cggggcgatc ggcggcgacc ctaggcgaca
    11161 ccgcgaggtc ggccggcagt gacccgccta cgtggtgcac gatgtcaaga ggcgccgctc
    11221 tcctgtgcgc aagcgcttac cctgtgcgcg acgtcgggct cacgcccaca agtccgcggc
    11281 tccgttgcgt tcggcgagcg accattggta tgcacggagg gcacggaccc atggagttca
    11341 cgctctacga agctggcggt cgcgccgtgg cgtactgcga cgacggtcga cacgtctatg
    11401 cgttccatgg agcaccgctg gcctacattg acggcgactc agtctatgcg ttcgatggcg
    11461 aacaccttgg ctggtgggat caaggctggg tccgcgatca ccacggtgct tgcgcattct
    11521 tcaccgaagg ggccgtcgcc agtgggctgg cgttaccgcc ttgacaggag cgtccgttca
    11581 aggccacaaa gcaggcaccg cgtgctcccg cgtaccctct gccgaaaccg gctcgcgcgc
    11641 acgacgagcc ggcctggtcg ctgcgctcga gtacacagtt ctttccgcgg ctacgctgag
    11701 tctgaccctc cccgaactgg agcacctggt caacggaaag cagcgagccc ccggcgagcg
    11761 ctgcgaaagc cgccgtgtac ggccccatac cgcgttctag caactgccaa tcgtcgccag
    11821 tcgatcacga cgcagacgag gcgctaggca acgtgaaatg gaagacgctg ccgcgtccca
    11881 gctcgctttc tgcccagatc cggccaccgt gcgactcgat aatacacttc gagatgtata
    11941 ggccgagccc aagaccccgc ctcgtcgtcg tcggagcctg ccagaaccga tcgaagatcg
    12001 cggccaggtg tttgtgggcg atgcccggcc cggtgtcgcg aaccgagaac aacacttcgt
    12061 ccccctgacg ctgcacggcg atttcgatcc ggccctctcg ttcggtgaac ttgatggcat
    12121 tgccaacgag gttcgcgagc acttggagga tgcgctcttg atcgaacctc gccagcagtg
    12181 cgtcgccggc catgtccacg gtgagcgcga tgccgcgcgc gatggctacc ggttgaaacg
    12241 cgtccaaggt ctccgttagc aaacggcaag cgtcgagctt cgcgacgacg agtccaagct
    12301 ggcccgcttc gatgctggtg acatccaaca gatcaccgat cagccgtgtc atccgagtcg
    12361 taaagcgctg gatcgcgtcc gcgcgttgga cgactcgttg cccggcgttg tctggaggcg
    12421 cttcgtgcat cagcatcgct gcgttgaggc cgatgccagc gaggagcgtc cgaaggtcgt
    12481 ggctcaccat gccgagaaaa tcatcgcgcg ctgtgatcgc gctgtccgcg ctgcgccgct
    12541 cgctcagcaa gctcgcatcc gtgctctgac gttccgtctc gagcaaggcg gcgatgacgc
    12601 gatgacggtc agcacgctcg atgttgcgtt gacggtcggc gacctcgcgt tcgcgggcga
    12661 ggagatcatc cgcccgatga cgctcggccg cgaggcgacc acttccttcc gccgtctccg
    12721 gtgatcgctg gtcggctgcc tcgcgcgtgg cccgaacggt gtcgtcggcc ttcacccgcg
    12781 cgttagcgac ggccgcatcg gcatcatgct cgatgtccgc gcgacgccgc gcgagctcgt
    12841 cgtcggtctt gcgacgttcc tcgccgaggc tttggtctgt aacatcgcga ccgcttgcct
    12901 tctgattgtc cggcaccgtc gatgcatacc acgaacgtca tgctcgacgc ggtggaacct
    12961 ccgcgtcgcc cgcgggtcga caccgcaaac aaggtcgcgg cccgctgagc gcgacgagga
    13021 accacccaca agcgcgagct tggcccgggg tagaagtgga gccatccgac cgcctcggcg
    13081 ccccgatcac acgctccact gctgggcttc ggggatgccg ttcgcgaggg cggcccagcg
    13141 ctgcgccctc gccgccgccg caaccgcccc gcgggcgagc tctgcgcgca aacacccgca
    13201 cgatcgcgtg cggcctgcca gaagcgatgc ggcccgcacg accagctcgc atccgcaggt
    13261 acagcgccac agggggcggc gcggcgcctg gccggtgctg ccctcacgcg cgaggacggt
    13321 cagccgcccg aacgtgcggc cgaccaggtt gaccgcgggc tcatcgacca cctcgaagga
    13381 gggacttccc cggtgctgca gccacagcgc ggcggcgtac ggatgagctg cgcgattgat
    13441 agctcaccgc gcatcggcgt cgtcacgcgt caccggccgc gtacacgcgg ccgacagggc
    13501 tatgccgtca gtgatgcggc agctgcaggt ggagcagatg ccagccgacc acgacgacga
    13561 tgacgacgaa gaccgcgatc aggatccagg tcgcgatctc gtcccactgc atcgccggac
    13621 ggcggcggcg cacaacgcgc gcgggcggca gcggctgcgg ctgtggtgcg ggccgtgcgt
    13681 cgacggccat cgccatcacc taagtgttgc cggggatgcg gtaaccgtcg gcgcagaaca
    13741 cgtaccagct cggcgccgag gtcgtcggga aggcgacgaa ccggccgcga gcgtcgcgcg
    13801 acggtagcga gagcgcctgg cccgacgcgt cgccgggcat tgctggcgcg acgcgcgccg
    13861 tcttcggcgc cgcccccggc cgagccacgg aacgcccgcg tgcgtccccc ggcgggaggt
    13921 tgcgagcgcg ctgggtggcc ctgctctcgg tctcggtcat gtcccttcgc tattgcggat
    13981 gatgcgccgg aatcgggagc tcctaatatc ttatttgaca acagcgcagt tcgcgcagtc
    14041 ccttgacctg tggcgcgacc gcagcagcac tggccaagcg agttgccatt gcggatcccg
    14101 agctccatag tgcaccatgg gagctcggcc gccctcgctg tgtgtcgctg gatactcgtg
    14161 tcaccgggat cgttgccttt gcgccacagt tcactctcgt taaacacggc tcaggaccat
    14221 ggaatgcata tgatcggact caccatagtg gcattgatcg cgcaatgtca ctctgcacat
    14281 ggttccgggc gtccgcaaca tgtacctgag cgacgaggcc ggtcggatcg cgtaccaccg
    14341 ggccatgatc agccgtgtcc gatcgcgccg agctggctcg atgtgctgct cggcgtcaac
    14401 ctgcgagccg ccgggtagtc accagccgcg agcgggctgc ttggccgcca ggcactgagg
    14461 cgcgcggccc acggcgtgcg tcgccgatca ggcgcggcgc cgtcggcggc agcaacggcg
    14521 ctcacggcga gtgcttcgtc gacgtggcag cgccgcgcct gactggtacg ctgccgatcg
    14581 tgccctcaca tgccgtcaga atcgactgcg aggaaaagct atcaaccttg ctcgcgacgt
    14641 acgagcgtat ggtacatgcc agcgcgcgca agaccgaatg gatcgagcta gagatagcgg
    14701 tgaatcaagc gttcataaac gcgttggatg cagccgcgca accaactgcc gacagtgacg
    14761 gtgcgcggcg tatgcaggag cacatgaacc agctcgtctc gctacgccag cgcatcgagg
    14821 gcgagatcgc ttttgccaag gcgagggagg agcgccagct cggtgccggc cccggtgagc
    14881 ctgccgctcg acgccgtgtt gctgcatccg aggccgacgg ttcgggtcat tggcaagggc
    14941 cgacgcgagc gagtgctgcc gctgtggaag gagaccgccg cagacctacg cgcatggctg
    15001 cgcgttcgcg gcgacacgcc cggtgtcgtc gagctgttca caaacgctcg cggccagccg
    15061 atcacgcgcg cgggcctcgc atacgtgctt cgccaccacg cccggaccgc cgctgctcga
    15121 tgcaccacgc tctcgcgcaa gcgggtctct ccgcacgttc tccgacacac gtgcgcgctg
    15181 accatcctcc aagcgaccgg cgacctccgc aaggtgtcgc tgtggctcgg ccacgcggac
    15241 atgcaggcga ccatggccta cctgcgcgcc gacccagccg agaagctcgc cacggtggcc
    15301 gccgtcatgc cgatgcccgg tcgtcgggtc cggctgcgcg cgcccgacaa gctgctcgcg
    15361 tcgctccaca gccgctaatc acggaggcgt cggaacttcg cgcgcggtcg accactgcaa
    15421 gagcgcgtcg agcgcggggc atagcgactg gccccacggt gtcaacgcgt attcgaccct
    15481 gggcggcttc tcctgatgat cgatgcgacg aacgatcccg tcctgctcca gctgccgcag
    15541 ctgttgcccg agcatcttct ccgagatccc gggcaacgcc cgctcgagct cggagaacct
    15601 ctggcgtcga cccccgaaca ggtgaaacag gatccggagt ttccatcggc cgccgatgat
    15661 cgcgaaggcc gcctcgacgc cgtcggccgc cgtctcggga gtccattccc tcggcgagtc
    15721 ctggccgggg gtacttactt cgaggtgcgt tcttgtcacc atccgatgat gccccaagtt
    15781 ggcggcatgt cacgcaatct cgaaccatcc atcgcgcagt acatcgaagg tgtcaacgcg
    15841 ttcgacgccg acctggccgc aacgggcttc gccgtcgatg cggtcgttca cgatgagggc
    15901 cgcgatcacg ccggactggc tgctattcgc gcgtggatcc aggacgcgaa cacgcgctac
    15961 tcgacgcaga tcgccatcga cactttgacc cagcaaggcg acgccacggt cgttcacgcg
    16021 acgatctcgg ggcgtttccc gggcagcccg ctcccgctgc ggttctcgtt ccagctccac
    16081 gcaggccgca tccaaaggct ggagatcgcg ccatgagtgg caagcgtgcg ctcgtcaccg
    16141 gcggcaccaa gggcatcggc gaagcgatcg tgcgcgcatt gacccgcgac ggcgcacgcg
    16201 tcgcgaccac aggacgctcc ccgttgccgg ccggtcaggc accggcgctg ttcgttcagg
    16261 ccgatgtcag caccgcgcgc ggtgttgacg aggtggtatc ctgggtcatc gaccagctcg
    16321 gcggcgtcga catcctcgtc aacaacgtcg gcggctcgtc ggcgcccgct ggcgggttcc
    16381 aggcgctcgg cgacgacgac tggcagcacg cgctggacgc caacctgctg gcggctgttc
    16441 ggctcgatcg tgcactgctt ccagggatgc tccagcaggg ctctggcgtg atcgttcaca
    16501 tctcctcgat ccaacgccgc atgccactgt tcgaggccac gctcgcctac gcggcagcca
    16561 aggccgcgtt gacaacctac agcaagggcc tcgcgaatga ggtgggccct cgcgggatcc
    16621 gtgtcaacag tatcgctccg ggcttcacag agacgtccgc ggcagccagg ctgatagcaa
    16681 agctccgcga tcacgaaggc atcgacgaag cgcaagctcg ccagcgcctc gtggactcgc
    16741 tcggcgggat tccaatcggg cgtccagcgt tccccgatga ggtggcggag ctcgttgcgt
    16801 tcctggtgtc agcccgggcc ggagccatcc acggtagcga gtacacgatc gatggtggcg
    16861 cagtcccaac cgtctgaacg atatcgagaa gaagttggcg agcgtggagc tcccatcggg
    16921 gccatttcgc ccgatgttgg ctttgattat gcgaagcgat cagcgtgcta cctccgtcga
    16981 tggcgacgcg ctcgcggccg gtggcctcgg ataattctcg tcttcacata aaagaactta
    17041 tgctcagctc tccataagtt cttccacgat ccgggagcct atcgcgactg gcgcgcgggg
    17101 cgaccgttca acgtctgccg ctgggatcgc gacatgcgct ggatgtcgta ccggggcagc
    17161 gttccggact tcgtc
//
LOCUS       DASVOR010001836         1615 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_194885,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001836 DASVOR010000000
VERSION     DASVOR010001836.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194885"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(217..1128)
                     /locus_tag="VF516_34195"
     CDS             complement(217..1128)
                     /locus_tag="VF516_34195"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8112849.1"
                     /translation="MPQAKEIIMSTSLSRLALTAAALAGGTIALASSRTAHADGKLTA
                     TVFTGSPAGFLVDSTLVAGDKDAILIDGQFDLADAHRLVAMILDSKKNLTTVYVTHFH
                     PDHYFGLGVIQQAFPRAKLVALPAVVDEIKRTWQDKVKQWSPLYGDLVPSEPVLPAPL
                     TGSSLTLEGQTLEIHGGVQGDAEDNSYVWIPSIKTVVAGDILYRGVHAWTRETTAAQR
                     KAWRKTLDELAALHPTTVVAGHKDPKQKDDAGAIDATRAYLDTFDAAVASSKTSAEAQ
                     KKVKAKYPSAQLDIILQLGADAAFAAR"
     gene            complement(1160..>1615)
                     /locus_tag="VF516_34200"
     CDS             complement(1160..>1615)
                     /locus_tag="VF516_34200"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8112850.1"
                     /translation="ARPFDDVQAWLLAALLRVLVLRAERLVARLGVAALPTAPLQRFF
                     TILERDHQATRSVAHYARAAGISARRLAELVSRHAGKSTKQVIDERVILEQKRLLAHT
                     ELSVKELAARTGFAEPTNLVKFFRHHVGATPLEFRDRHRRGFLPSSRRS"
BASE COUNT          226 a          529 c          599 g          261 t
ORIGIN      
        1 atcgccttca ccctcgacat cgccggcgga ctcgacggcc tcgaccccga ggcgccgctc
       61 ctgtatcgcg ccaccgcgcc ggtctgcacc gacggctatg tcctcgagac ccgcgagctc
      121 tggagtgccg acggcagcct ggtggcaatc aaccaccaga ccttcgtgat catttgaaaa
      181 ttttcggcgg ttcttccgct cccggcggac gaggccctac ctggccgcga acgcggcgtc
      241 ggcgccgagc tggaggatga tgtcgagctg ggccgacggg tacttcgcct tgaccttctt
      301 ctgggcctcg gccgaggtct tcgacgaggc gaccgcggcg tcgaacgtat cgagataggc
      361 gcgggtggcg tcgatcgcgc cggcgtcgtc cttctgcttc ggatccttgt ggccggcgac
      421 cacggtggtg gggtggagcg cggcgagctc gtcgagcgtc ttgcgccagg ccttgcgctg
      481 cgcggcggtg gtctcccgcg tccacgcgtg gacgccgcgg tagaggatgt cgccggcgac
      541 cacggtcttg atcgacggga tccacacgta ggagttgtcc tcggcgtcgc cctgcacgcc
      601 gccgtggatc tccagggtct ggccctcgag ggtcagcgag ctgccggtga gcggcgccgg
      661 cagcaccggc tcgctcggca cgagatcgcc gtacagcggc gaccactgct tgaccttgtc
      721 ctgccaggtc cgcttgatct cgtcgaccac ggccggcagc gcgaccagct tggcccgggg
      781 gaacgcctgc tggatcacgc cgaggccgaa gtagtgatcg gggtggaagt gggtgacgta
      841 gaccgtggtc aggttcttct tgctgtcgag gatcatcgcg accagccggt gggcgtccgc
      901 gagatcgaac tggccgtcga tcaggatcgc gtccttgtcg ccggcgacca gcgtcgagtc
      961 gaccaggaag ccggcgggcg agccggtgaa cacggtggcg gtgagcttgc cgtcggcgtg
     1021 cgcggtgcgc gagctggcga gcgcgatggt gcccccggcg agcgcggcgg cggtgagggc
     1081 gaggcgggac agtgacgtcg acatgatgat ctccttggct tgtggcacca gggtagtcat
     1141 cgcgcctcga cgggcgaggt caggatcggc ggctcgatgg taagaatccg cggcggtggc
     1201 gatcgcggaa ctccagcggc gtggcgccga cgtggtggcg gaagaacttc acgaggttgg
     1261 tcggctcggc gaatccggtc cgtgccgcga gctccttgac cgacagctcg gtgtgggcca
     1321 gcaggcgctt ctgctcgagg attacccgct cgtcgatcac ctgcttggtc gacttgccgg
     1381 cgtggcgcga taccagctcg gccagccgcc gcgccgagat cccggccgcg cgggcgtagt
     1441 gcgcgaccga ccgcgtggcc tggtggtcgc gctccaggat ggtaaaaaac cgctgcagcg
     1501 gcgcggtcgg cagcgccgcg acgccgagcc gcgcgaccag ccgctcggcc cgcagcacga
     1561 gcacgcggag cagcgcggcg agcagccagg cctggacgtc gtcgaacggc cgggc
//
LOCUS       DASVOR010001837         1721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_107803,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001837 DASVOR010000000
VERSION     DASVOR010001837.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107803"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..823)
                     /locus_tag="VF516_34205"
     CDS             complement(134..823)
                     /locus_tag="VF516_34205"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8112851.1"
                     /translation="MARKRSPRKPAATGDEAEAAATGKRAGRDRKKPPQPMITVRHMT
                     RRDIGRVWDFLKISFREVNAETVEYQRPRSKHRFEEVYEEEGIEQLVFEIGDDIVAYA
                     ECSYEVTGSDNWINPRYFEKRNMRPLFVDELAVHPEMHGRGVGSFVLEQLHHLARVHG
                     LNHLVLEVAENNQRALDWYKKRSFKKLDAAIFMAAPVDIEPELLPPRELPPVRTEPET
                     PEPSESDPTGE"
     gene            complement(896..1468)
                     /gene="def"
                     /locus_tag="VF516_34210"
     CDS             complement(896..1468)
                     /gene="def"
                     /locus_tag="VF516_34210"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX8112852.1"
                     /translation="MAIRKIAQIGHPVLRQVAREVGRDELARPETQRFIDDLIETMHD
                     ADGAGLAAIQVYEPIQICAIEVRNNPRYPYKPPIPLTVLVNPVLTPVGDEQFDNYEGC
                     LSVPNLRGVVKRAVHVHVRAWDRHGNPIDEVVHGLKAGTYQHEVDHLRGKLFVDRADP
                     STFTTWTEFDRHHRARFVEQVTQLVARVGS"
     gene            complement(1492..>1721)
                     /locus_tag="VF516_34215"
     CDS             complement(1492..>1721)
                     /locus_tag="VF516_34215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase (quinone)"
                     /protein_id="HEX8112853.1"
                     /translation="LRARLGDRVTLIAVGGIETADDAWQRIRAGASLVQAYTGFIYGG
                     PTWPRRIHKQLADKVRAAHLQSIEQAIGSDR"
BASE COUNT          228 a          577 c          635 g          281 t
ORIGIN      
        1 cggccggctg gctgctgcac cgccgcacgt ccacgtgcac accatcgacg caggccactg
       61 gctgcacatc gaggcgccgg cggcggtggt cgacctgttc gccgcgcggc tgccgtaagc
      121 gacgcggcgc gcgctactcg ccggtggggt cgctctcgct cggctcgggc gtctcgggct
      181 cggtgcgcac cggcggcagc tcgcgcggcg gcaacagctc gggctcgatg tcgaccggcg
      241 ccgccatgaa gatcgccgcg tcgagcttct tgaacgaccg cttcttgtac cagtcgagcg
      301 cgcgctggtt gttctcggcg acctcgagca ccaggtggtt gagcccgtgg acgcgcgcga
      361 ggtggtggag ctgctcgagc acgaacgagc cgacgccgcg cccgtgcatc tcgggatgga
      421 cggcgagctc gtcgacgaac agcgggcgca tgttgcgctt ctcgaaatag cgcgggttga
      481 tccagttgtc cgagccggtg acctcgtagc tgcactcggc gtacgcgacg atgtcgtccc
      541 cgatctcgaa caccagctgc tcgatgccct cctcctcgta gacctcctcg aagcggtgct
      601 tggagcgcgg ccgctggtac tcgacggtct cggcgttgac ctcgcggaag ctgatcttca
      661 ggaagtccca cacccggccg atgtcgcgcc gggtcatgtg gcgcacggtg atcatcggct
      721 gcggcggctt cttccggtcg cggccggcgc gcttgcccgt cgcggcggcc tcggcctcgt
      781 cgccggtggc cgcgggcttg cgtgggctgc gcttgcgcgc catcgcctca gcaaaccgca
      841 gcgccggggg tgggagcaag ctgcagcgcg aagaaaagcc agtcgcgacg ccgcgctacg
      901 agccaacccg agccacgagc tgggtgacct gctcgacgaa ccgggcgcgg tggtggcggt
      961 cgaactcggt ccaggtcgtg aacgtgctgg ggtcggcgcg gtcgacgaac agcttgccgc
     1021 ggaggtgatc gacctcgtgc tggtaggtcc cggccttgag cccgtggacc acctcgtcga
     1081 tcgggttgcc gtggcggtcc cacgcgcgga cgtggacgtg gaccgcgcgc ttgaccacgc
     1141 cgcgcaggtt ggggaccgac aggcagccct cgtagttgtc gaactgctcg tcgccgaccg
     1201 gcgtgagcac ggggttgacc aggacggtca gcgggatcgg cggcttgtag ggatagcgcg
     1261 ggttgttgcg gacctcgatc gcgcagatct ggatcggctc gtagacctgg atcgcggcca
     1321 gcccggcgcc gtcggcgtcg tgcatggtct cgatcaggtc gtcgatgaag cgctgggtct
     1381 cgggccgcgc gagctcgtcg cgcccgacct cgcgggcgac ctggcgcagc acgggatgac
     1441 cgatctgggc gatcttgcga atggccatgg ccggctccga ctgtagcagg atcaccgatc
     1501 gctgccgatc gcctgctcga tcgactgcag gtgtgcggcg cggaccttgt cggcgagctg
     1561 cttgtggatc cggcgcggcc aggtcgggcc gccgtagatg aagccggtgt aggcctggac
     1621 cagcgacgcg ccggcgcgga tccgctgcca ggcgtcgtcg gcggtctcga tgccgccgac
     1681 cgcgatcagc gtgacccgat cgccgagccg ggcgcgcagc c
//
LOCUS       DASVOR010001838         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_223969,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001838 DASVOR010000000
VERSION     DASVOR010001838.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223969"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VF516_34220"
     CDS             <1..1348
                     /locus_tag="VF516_34220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112854.1"
                     /translation="AAAAAPPDARAQPAAEARNMEQVAQLDLAGNGDGGEGLALQQRP
                     DGRRILYLAHEGQKTCLSVVDVTNPRAPTLVAQLPSPGPGVTRCNSLGLSGNVLAVAN
                     QTQKVGQKPAGMWLLDVSDLARVQQAHALDDLKLAFFDTSGPQSRGVHWLWFVDGEFA
                     HLATGTADFKPTNPGDDQFYQVVDVRNPRAPREVGRWWLPGTRTGDPCLPGCLPRRQK
                     IDDGYRAHSIQIYPQRPDRAYVGYIDGGMLILDISKLADVRAGKAPRFSPRLISRLDY
                     GPPYPSWCHTVQPLPGRGLAVVSDEAVQDGCTDAPKLIWLVDIREETSPLIIGTAPLP
                     DNVAALCTRGGRFGAHNLHPAFPDGVSAQLKNTFVGTFFNGGVRIYRLIDPALPGAPP
                     QITEIGYFIPAAPARNPGKTAQINHVIVDEKGLIYADDRVTGGLYILKYTGKPPLD"
     gene            complement(1865..>2480)
                     /locus_tag="VF516_34225"
     CDS             complement(1865..>2480)
                     /locus_tag="VF516_34225"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8112855.1"
                     /translation="SLYNRAMSILLIRHGETAANAARIMQRPDVPLSARGIRQAAQLA
                     ERLFALGFAHVLCSDLVRARMTAAPLAARGHEIEGTPLLQERNFGDLRGTPYAQLTED
                     PFAPGFVPPGGESVEVFHRRVVQAFALIVERRRALSGALVVVTHGLVCGALLRNHARA
                     AAVPDGFANAGLTVLDPDPPFEARLINCTQHLVAELGDTGGGPV"
BASE COUNT          396 a          937 c          811 g          336 t
ORIGIN      
        1 cgcggccgcc gccgccccgc cggacgcgcg cgcccagccc gcggccgagg ccaggaacat
       61 ggagcaggtg gcacagctcg acctcgccgg caacggcgac ggcggcgagg gcctggcgct
      121 gcagcagcgc cccgacggcc gccgcatcct ctacctcgcg cacgagggcc agaagacctg
      181 cctcagcgtc gtcgacgtca ccaacccgcg ggcgcccacg ctcgtcgcgc agctgccgtc
      241 gccggggccc ggcgtcacgc gctgcaactc gctggggctg tccggcaacg tgctcgcggt
      301 cgccaaccag acccagaagg tcggccagaa gccggccggc atgtggctgc tcgacgtgtc
      361 cgatctcgcg cgcgtccagc aggcccacgc gctcgacgac ctcaagctcg cgttcttcga
      421 cacctcgggc ccgcagtcgc gcggcgtcca ctggctgtgg ttcgtcgatg gcgagttcgc
      481 tcacctcgcg accggcacgg ccgacttcaa gccgaccaac cccggtgacg atcagttcta
      541 tcaagtcgtc gacgtccgca acccgcgcgc gccgcgcgag gtcgggcgct ggtggctccc
      601 cggcacgcgg accggcgatc cctgcctgcc cggctgcctg cccaggcgcc agaagatcga
      661 cgacggctac cgcgcccaca gcatccagat ctacccccag cgacccgatc gcgcgtacgt
      721 cggctacatc gacggcggca tgctgatcct cgacatctcc aagctcgccg acgtgcgggc
      781 cggcaaggcg ccgcggttct cgccgcgcct gatcagccgg ctcgactacg gcccgccgta
      841 tccgtcgtgg tgccataccg tgcagccgct tccgggccgc ggcctggcgg tggtgtccga
      901 cgaggcggtg caggacggct gcaccgatgc ccccaagctg atctggctgg tcgacatccg
      961 ggaggagacc agcccgctga tcatcggcac cgcgccgctg ccggacaacg tcgccgccct
     1021 gtgcacgcgc ggcggccggt tcggcgccca caacttgcac ccggcgttcc ccgacggcgt
     1081 atcggcgcag ctcaagaaca cgttcgtcgg gacattcttc aacggcggcg tgcggatcta
     1141 ccggctgatc gaccccgcgt tgcccggcgc gccgccgcag atcaccgaga tcggctactt
     1201 catcccggcg gcgccggccc gcaatcccgg caagaccgcg cagatcaacc acgtgatcgt
     1261 cgacgagaaa ggcctgatct acgccgacga ccgcgtcacc ggcgggctgt acatcctcaa
     1321 gtacaccggc aagccgccgc tcgactgagc gccagccgcg acccatacta aggtgtcctc
     1381 gcgcaacgaa tcccgaagct gtaggcacga acaactggag tgccgaggtt gcggaaggac
     1441 gcgcgcaggc ccgacgcatc aatgccgaac gagccacccc gaagcacacg gtcgattcca
     1501 gtcgtcacat tcgcacaatc actgcacgga tttggataac caccagcata ccgatctagc
     1561 gtccattccc acacgtttcc cgcaagatca gcccatcttc ataggccccc accaggtctt
     1621 ggtgtccttt cacgcgccgc gccccatccg ctgttcagcg gatccggatg gggctcgaac
     1681 agctcaacgt gtcatgggcg cactatcgcg attctcgcat atctgcaatc tgctacggcg
     1741 cgcgcgcagc gactgcgatc tcgccccttg aacctgccgt cggcccagcg cgagccgcaa
     1801 cctttccgcg gttcgatcag ggcggcgaga aggctcgccc agcagggaca cgcgccgccg
     1861 gcgatcacac cgggccgccg ccggtgtcgc cgagctccgc taccaggtgc tgcgtgcagt
     1921 tgatcaaccg ggcctcgaac ggcggatcgg ggtcgagcac ggtcagcccc gcgttggcga
     1981 agccgtccgg cacggcggcc gcccgggcgt gattcctgag cagcgcgccg cacaccaggc
     2041 cgtgggtcac gaccaccagc gcaccggaca gcgcgcggcg gcgctcgacg atcagcgcga
     2101 acgcctgaac gacccggcgg tggaacacct cgacgctctc gccgcccggc ggcacgaagc
     2161 cgggcgcgaa cgggtcctcg gtcagctggg cgtacggcgt gccgcgcagg tcgccgaagt
     2221 tgcgctcctg gagtagcggc gtgccctcga tctcgtggcc gcgcgcggcg agcggcgccg
     2281 cggtcatccg cgcgcgcacg aggtcgctgc acagcacgtg ggcgaacccc agcgcgaaca
     2341 gccgctcggc gagctgcgcc gcctgacgga tgccgcgcgc gctgagcggg acgtccggcc
     2401 gctgcatgat gcgggcggcg ttggcggcgg tctcgccgtg gcggatcagc aggatcgaca
     2461 tggcgcggtt gtacagcgaa
//
LOCUS       DASVOR010001839         2212 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340325,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001839 DASVOR010000000
VERSION     DASVOR010001839.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340325"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1023..1598
                     /locus_tag="VF516_34230"
     CDS             1023..1598
                     /locus_tag="VF516_34230"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8112856.1"
                     /translation="MRVAPEVGSLRRRAMYKAVREATITAALRERFRIVHVSLQRTHV
                     HLLIEADHKLALARGMQGFTISAARNINTALGDGVRRRRGRVFADRYHVEVITTPTRA
                     RHALGYILSNWRKHGEDRDGLPSTWLVDPFSSAISFPDWQELQDQPWMWPVRETYDPL
                     IVRRPQTWLLREGWKLGGGPISARDVPSRPH"
     gene            1932..2117
                     /locus_tag="VF516_34235"
     CDS             1932..2117
                     /locus_tag="VF516_34235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112857.1"
                     /translation="MCMAGNMKDWNKDFYGGNPFFETPFDGPCPKYCKKGDRPPKSDY
                     GDVQLPANTMYPTCKPV"
BASE COUNT          428 a          713 c          709 g          362 t
ORIGIN      
        1 agcgcgcgct tgcaaccgca gtacccctgc tgggctcgcg tgcaggaaca agtttcgcgg
       61 ggttgaacgt gccgggtatg gcacccgcgc tcgatgtggt gatcacgctc cgaccatcac
      121 ggatcgcacg agtcggcgca gcccgatcgg cgttctcgtg gaccggcgcg gctgcagcgt
      181 tcgtgagacc gccgccactg ttcgtcgcga tgcgctggcc aacagccgac gagagaccac
      241 cgagatcgtc gcatgcgact ccggacaagg acacgggaga ccgcgtggcc agccctgtga
      301 ctggcccgcc gtagccgacc atgtccccac gacggcccac tcacagcgca tcgccccgcg
      361 cgatgtctcc cttccctgat ttgattgatc ttgttccgga tgggaacgct tctatatcgc
      421 cgaagcagat cgacgagtca atccgatccg cacatgtgac gctgatctga ttctcagtgc
      481 gactctctca acaagagatc aaaaatataa atgcaagaat ggagcctgag gtcgagggca
      541 aggactgggg ggaacggaag gacgactgtg agccgcggcg ccgttgcctt ggtttgccgc
      601 ggcgcgggtc atctgatcgt ctttggcgtt ccggccacga cttcccaggc agtacgcgcg
      661 tgtgatcggg tgcaagcgag gcaatgatgg atcgcgcagc aaggcgaatg acccattcgt
      721 atccatctca cgacaggtgt ctcgacctga atgcttgagg catcgcgttc cggatctgat
      781 aatcgacgaa tatgaatggc cattactacc gcccaatcat tgacaggctg cgcagtcgtg
      841 gtaatgagtg aggcatgaag tcgtcgaggt cgagccacaa gccaaggcgg aatgctcagc
      901 aggaactgtt tcgacgcggc ggcaagcgac gtggggcggg ccgcaagccg aagagctcgc
      961 gggccaacga gcggcacgcg gcgcgacctg agttcgagcc gcatcatccg ctgcacgtgg
     1021 tgatgcgagt cgcgccggag gtcggcagcc tgcgccgacg cgcgatgtac aaggcggtcc
     1081 gcgaggccac catcaccgcc gcgctacgcg agcggttccg gatcgtccac gtctccctgc
     1141 agcgcacgca cgtccacctg ctcatcgagg ccgatcacaa gctcgcgctt gctcgcggga
     1201 tgcagggctt cacgatctcg gcggcgagga acatcaacac cgcgctcggg gacggtgtac
     1261 gccgtcgccg cggcagggtg tttgccgatc gctatcatgt cgaggtgatc acgacgccca
     1321 cgcgggcgcg ccacgctctg ggctacatcc tctccaactg gagaaagcat ggggaagacc
     1381 gcgacggatt gccgagcacg tggctcgtgg acccgttctc cagtgcgatc tcgttccccg
     1441 actggcagga gctacaagac cagccctgga tgtggccggt ccgcgagacc tacgatccgc
     1501 tgatcgttcg ccggccacaa acctggttgc tccgagaggg ctggaagctc ggcggaggtc
     1561 cgatcagcgc gcgcgacgta cccagcaggc cacattgatc ggtgtcgagc ggggtcggct
     1621 ccgccgacag gccgtcgatc atcgccatcg cgccagcggt gtcaacgtac gcacagctgg
     1681 ccgctgcctt gagagcggcg cgcacgatcg cggtctgccc ggcgtcgacc tggaccttct
     1741 gctcgagctg ctcgtaacgt cgagctggac cgtcgagctc gcggtcgccg gcggtcacgc
     1801 cggactcgac caccggctgc gactgcttca agaacacgcc gaccccgacc tgcgagaagg
     1861 gcctgatcac actgcccgac gggctcaatg cctgccagaa ggcgtgcgac gaggagctca
     1921 agaagaacac catgtgcatg gcgggcaaca tgaaggactg gaacaaggac ttctacggcg
     1981 ggaacccgtt ctttgagacg ccattcgatg ggccgtgccc caagtactgc aagaagggcg
     2041 accggccgcc gaagagcgac tacggcgacg tccagctgcc ggcaaatacc atgtacccga
     2101 cctgcaagcc ggtctaggag gacaacccgc cgggctcccg gctccggcag cagctccggc
     2161 tcgggcagca gctccgactc cgacaacgcg tggtactact acttctgagc cg
//
LOCUS       DASVOR010001840         2149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_281390,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001840 DASVOR010000000
VERSION     DASVOR010001840.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281390"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..721
                     /gene="apaG"
                     /locus_tag="VF516_34240"
     CDS             293..721
                     /gene="apaG"
                     /locus_tag="VF516_34240"
                     /inference="COORDINATES: protein motif:HMM:NF003967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEX8112858.1"
                     /translation="MRPTVAEPTSCAETNGIRVRVQSHYLAEQSSPREERYAFAYTIT
                     ISNEGTRSAQLRTRHWIITDVCGTVEEVRGDGVVGKQPRLLPGQSFQYTSSCALATPV
                     GTMHGTYRMWRDDGTYFDAEIAPFSLAVPASQRTGAHEAN"
     gene            803..1465
                     /locus_tag="VF516_34245"
     CDS             803..1465
                     /locus_tag="VF516_34245"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8112859.1"
                     /translation="MTHAEREAATATALVDAAKAGDQSAFAALVTRYRKRIFALALHI
                     TGSASEADDIAQDVFLKAYRALPEFEGRSQFFTWVYRMTVNRSLNVRRDRARRGEDLL
                     EDPRLELAVAVDARSDPGREAELRQSYARLLRALDALPADMRTTVILVSLPGLAYGEV
                     AVVQKISEGTVAWRMHEARRRLNEAMMPRKLQRRRELSAELSRVLVEHGLFVPVMPKN
                     RA"
     gene            complement(1723..>2149)
                     /locus_tag="VF516_34250"
     CDS             complement(1723..>2149)
                     /locus_tag="VF516_34250"
                     /inference="COORDINATES: protein motif:HMM:NF025850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8112860.1"
                     /translation="ALAPAPGTPAYRHYRYFMHYAEGSLMPFLVMKLVFNKIKTDAPF
                     LVRPIAKAIAGQVSKQFIDPNVAAHLAFLADHLTKHEWFAGDAFSAADVQMSFSVEGA
                     IARAGGGISPKLTEWLAKIQARPAYRKAIEKGGEHKVAG"
BASE COUNT          323 a          768 c          735 g          323 t
ORIGIN      
        1 cgatcctggc cccgggcgcc ggtgcgtccg gcagctgtcc ccagggctcg tcgggggggc
       61 cgcccggcgc gcttgctacg accacggcgg gcggggcctg ggccccgggc gagctcgcca
      121 ccgggctgca ggcaccggtc agcgcgattc cgatccagat ccgcatgcgc gactctctcg
      181 tcggcggacg gcgcatgggc tctagcatga cacgccccgg agcggcatcg ggtcgatccc
      241 acggggggcg ttgacccttg catgtccacc gacatcgcgc gagattgccc acgtgcgtcc
      301 caccgtcgcc gaacccacct cctgcgcgga gaccaacggg atccgcgtcc gtgtccaatc
      361 gcactacctc gccgagcaat cgtcgccgcg cgaggagcgc tacgcgttcg cgtacacgat
      421 cacgatctcc aacgagggta cgcgcagcgc gcagctgcgc acccggcact ggatcatcac
      481 cgacgtctgc ggcacggtcg aggaggtccg cggcgacggc gtggtcggca agcaaccgcg
      541 gctgctgccc ggccagagct tccagtacac ctcgagctgc gcgctcgcca ccccggtcgg
      601 cacgatgcac ggcacgtatc ggatgtggcg cgacgacggc acctacttcg acgcggagat
      661 cgccccgttc tcgctcgcgg tgccggcgtc ccagcgcacc ggcgcacacg aagcgaactg
      721 attgatttcc gtgacttgcg cctgcgcgtg gccggcgcaa caaaccgcaa agcattcgcg
      781 taggctgtgc gtctaatccg cgatgacgca cgccgagcgt gaagccgcca ccgcgacggc
      841 cctggtcgac gccgcgaagg cgggcgatca gagtgcgttc gcggcgctgg tgacccgcta
      901 ccgcaagcgg atcttcgcgc tcgcgctcca catcaccggc tcggcgagcg aggccgacga
      961 catcgcgcag gacgtgttcc tcaaggccta ccgcgcgttg cccgagttcg agggccgcag
     1021 ccagttcttc acctgggtct accggatgac ggtcaaccgc tcgctcaacg tgcgccgcga
     1081 ccgcgcccgc cgcggcgagg acctgctgga ggacccgcgc ctggagctcg ccgtcgcggt
     1141 cgatgcgcgc tccgaccccg gccgcgaggc cgagctgcgc cagagctacg cgcgcctgct
     1201 gcgcgcgctc gacgcgctgc ccgccgacat gcgcaccacg gtgatcctgg tgtcgctgcc
     1261 gggcctggcg tacggcgagg tcgcggtggt ccagaagatc agcgagggca ccgtggcgtg
     1321 gcggatgcac gaggcgcggc gccggctgaa cgaggcgatg atgccccgga agctgcagcg
     1381 ccggcgcgag ctatcggccg agctgtcgcg cgtgctcgtc gagcacggcc tgttcgtgcc
     1441 ggtgatgccc aagaaccgcg catagcatcg acgggtggac gtcgacctgg gccttgccct
     1501 tgacgtcgac cttgactcca acgttgaggt tgacggcagc gaagcgatac gtagacgtct
     1561 gaccccaccg tcgacctcga cctcgaccct gccgtcgctc gtcgaggctc gcagcacagg
     1621 atcggtcgac gtccaacgtc gatggtgggc tcaacgtcgt acgtcgccgt acgtcgccgt
     1681 cgacctcaac gctccgggtc aacgatcagg tcgacgtcga cgctaccccg cgaccttgtg
     1741 ctcgccgccc ttctcgatcg ccttgcggta cgccgggcgc gcctggatct tggcgagcca
     1801 ctcggtcagc ttgggcgaga tcccgccgcc ggcgcgggcg atcgcgccct cgaccgagaa
     1861 gctcatctgc acgtcggccg cgctgaacgc gtcgccggcg aaccattcgt gcttcgtgag
     1921 gtgatcggcg aggaacgcca ggtgcgcggc cacgttgggg tcgatgaact gcttggacac
     1981 ctggccggcg atcgccttcg cgatcggtcg gaccaggaac ggcgcgtcgg tcttgatctt
     2041 gttgaacacc agcttcatga ccaggaacgg catcagcgag ccctccgcgt agtgcatgaa
     2101 gtagcggtag tggcggtacg cgggcgtgcc cggcgccggg gccagcgcg
//
LOCUS       DASVOR010001841         2112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_223992,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001841 DASVOR010000000
VERSION     DASVOR010001841.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2112
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223992"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="VF516_34255"
     CDS             complement(<1..1352)
                     /locus_tag="VF516_34255"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HEX8112861.1"
                     /translation="MTTLARTLVMVIVMAGCGGNPAMPDAPDEMAMLAATDASGAPLT
                     SIDVPLTLVGQAASVGVVVHNTGTAETGAISISIGGANAGDLAIDAARTTCAATSLAP
                     GATCTVELVFHAAAAGDRTATLTIASPAGGTLELGITAHGGVPDLQLAPTVELGNVEV
                     GSAAQVSVVLRNAGTFAAPIDAITVSSTGFSAGVFSLGATTCGASLGAHAMCTIDVVA
                     SPRDMAPIPFAGTLVVASSGVDHTAALTARGAHRVKILKSGTVTGTVTLTEVVAQPTS
                     DTCGDTCEILTPYAILLTQTPDPGAEFLSWSLPDSGAGDCGMNPTCFVGKELTPTTVT
                     ATWVPAGSTQLHIAIVGGSGGLRIVSGGVTTTCLGECTTFVSPGPVTLTVEGTAPAIG
                     FSGACTSSGTQCTYTQGAGLSDVTVTLDNPNAVWTYLPGNGTDVRSAAFDGSGDLIIT
                     T"
     gene            complement(1370..2044)
                     /locus_tag="VF516_34260"
     CDS             complement(1370..2044)
                     /locus_tag="VF516_34260"
                     /inference="COORDINATES: protein motif:HMM:NF016041.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEX8112862.1"
                     /translation="MTRFEAIMVSRANYVAGIVFDCVGSLAAVVAGFVLGARLGPALV
                     VIAAAALWYTLVEYAVHRWIYHAGDSVMAVVHDRHHAEPKVMLGAPFYCSVSIWGLQG
                     LAAGVLLGAPLGLVFGGTLLFSYGQQTLIHHAAHRYPRLDVLGARSALRRHHAVHHVV
                     GDANFGVSTTLWDRVLGTYVTDLLGSAPVRPWWSRSRAWRVTRMELWRRTGAQDRSDD
                     SAAWSR"
BASE COUNT          350 a          733 c          734 g          295 t
ORIGIN      
        1 gtggtgatga tcaggtcccc ggacccatcg aaggctgccg atcgaacgtc ggtcccgttc
       61 ccgggcaagt acgtccacac ggcgttcggg ttgtcgagcg tcaccgtgac gtcgctgagc
      121 cctgccccct gggtgtacgt gcattgcgtg ccactgctgg tgcacgcacc ggagaacccg
      181 atcgcgggtg cggtcccctc gacggtcaac gtgaccggtc cggggctgac gaaggtcgtg
      241 cattcaccca ggcaggtcgt cgtgacaccg cccgacacga tccggaggcc accggagcca
      301 ccgacgatcg cgatgtggag ctgcgtcgag ccggccggta cccacgtcgc cgtcacggtg
      361 gtgggggtga gctccttgcc aacaaagcac gtcgggttca tcccgcaatc gccggcgccg
      421 ctgtcgggaa gcgaccagct caggaactcg gcgccgggat ccggcgtctg ggtgaggagg
      481 atcgcatacg gggtcaggat ctcgcaggtg tcgccgcagg tgtcgctcgt cggctgcgcc
      541 acgacctcgg tcaaggtgac cgtcccggtg acggttccag atttgaggat cttgacccgg
      601 tgcgcgccgc gcgccgtcag cgccgccgtg tgatcgacgc cgctcgaggc gaccacgagg
      661 gttcccgcaa acgggatcgg agccatgtcg cgcgggctgg ccacgacgtc gatcgtgcac
      721 attgcatgcg cgccgagcga cgcgccacag gtcgttgcac cgagcgagaa cacgccggcg
      781 gagaacccgg tgctcgacac ggtgatggcg tcgatcgggg cggcgaacgt gcctgcgttg
      841 cgcagcacga ccgagacctg cgccgcgctg ccgacctcga cgttgccgag ctcgacggtg
      901 ggggcgagct ggaggtccgg cacgccgccg tgggcggtga tcccgagctc gagcgtcccg
      961 ccggcggggc tcgcgatcgt caaggtcgcg gtccgatcgc cggcagccgc ggcgtggaac
     1021 accagctcga ccgtgcaggt ggcgcccggc gcgagcgacg tcgcagcgca ggtcgtcctc
     1081 gcagcgtcga tcgcgagatc gccggcattc gcgccgccga tcgagatcga gatcgcgccg
     1141 gtctcggccg tccccgtgtt gtggaccacc acgccgacac tcgccgcctg gccgaccagg
     1201 gtcagcggga catcgatgga ggtcagcgga gcaccgctcg catcggtggc agcaagcatg
     1261 gccatctcat cgggcgcatc gggcatggca gggtttccgc cgcagcccgc catcacgatc
     1321 accatcacca gcgttcttgc cagagttgtc atcgcgcacc gaggatagct cagcgagacc
     1381 acgccgccga gtcatcggat cggtcttgcg cacctgtcct tcgccacaac tccatccgcg
     1441 tgacgcgcca ggctcgggag cggctccacc acggccggac cggcgcagaa cccagcagat
     1501 cggtgacgta cgtgccgagc acgcgatccc acagcgtcgt cgacacgccg aagttcgcat
     1561 caccgacgac gtgatggacc gcgtggtgcc gccgcagcgc actgcgcgcg ccgagtacgt
     1621 cgagccgcgg gtagcggtgc gccgcgtggt ggatcagggt ctgctggcca tagctgaaca
     1681 gcagcgtgcc gccgaacacg agcccgagcg gcgcgccgag cagcacgccg gcggcgaggc
     1741 cctgcagtcc ccagatcgag acgctgcagt agaacggcgc gccgagcatc accttgggct
     1801 cggcgtggtg ccggtcatgg acgaccgcca tcaccgagtc gcccgcgtga tagatccagc
     1861 gatgaaccgc gtactcgacg agcgtgtacc acagcgcagc cgcggcgatc acgacgagcg
     1921 cgggcccgag gcgcgcgccg agcacgaacc cggcgacgac cgcggcgagc gagccgacgc
     1981 agtcgaacac aatgccggcg acgtagttcg cacggctcac catgatcgcc tcgaacctgg
     2041 tcatcgcgca ccattccagc acagaccggc gagctcgcga taggggtcat cgcgagcgcc
     2101 gtcatgcgag ct
//
LOCUS       DASVOR010001842         5885 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_281392,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001842 DASVOR010000000
VERSION     DASVOR010001842.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281392"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2432)
                     /locus_tag="VF516_34265"
     CDS             complement(<1..2432)
                     /locus_tag="VF516_34265"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8112863.1"
                     /translation="MAMLIAIGGVLSYLRTPTDIFPSINIPVISVLFRYSGLQPEDVE
                     KRIVNNFERFLTTIVSDIDHMESLSVTGQAIVKIYLQPGANVSQAIAQTTAISQTAVR
                     SMPPGMVPPLIMQYSATAVPIMQIAMESDSLSEQQLFDYGINFIRAEIATIPGAQIPY
                     PYGGKQRQIMVDIDPQRLHALGLSPRDVQNALAVQNVVLPSGTAKLGTNEYPIFVAST
                     PETLAELAGLPIKTVDGRTVYIRDVANVRDGNAPQTNMVHVEGRRSVLMTILKNGDAS
                     TLEVNERIREAIPRALDRLPKEAQGHLQVKMLFDQSIFVRASIDGVIREALIAAGLTG
                     LMILMFLGSWRSTLTVVISIPLSILFSIVVLHALGETLNVMTLGGLALAVGILVDDAT
                     VAIENIHRNMHQRKPFTRAIIDGAQQIAVPAFVSTLCICIVFAPIAFLTGAAKSLFVP
                     MALAVVFAMLMSYLLSRTLVPTMVHYLLKHESGGPNRFTVAFERGFSALRDSYGRRLA
                     WALQHRGFVVGGFAIIIAGSLALISLVGRDFFPTVDAGLIKLHVRDVPGTRLEESEKR
                     IGAIEQTIRGVIPPGEIETMLDVLGTPYSGINLSLSEGAAVSPADSQIMIALKPGHRP
                     TEDYLKALRERLHRVYPETTFFFLAPDISTQVLNFGLAAPIDLQVVGPIGSEDQTLAL
                     AEKLAAEVAKVPGAVDVHLAQESRVPQLQVAIDRVEAQQVGLTERDVANDLLVSLASS
                     SDVAPSFWIDRRGVQYTVAVQTPQYAIDSIDALRATPISTATNKVQTLGNLAQIKRVT
                     GPANITHSDIKRT"
     gene            2718..3830
                     /locus_tag="VF516_34270"
     CDS             2718..3830
                     /locus_tag="VF516_34270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112864.1"
                     /translation="MRRFAMVLSACLWACGDNQTGPPDLQPDAGFHPAPHAPLPTVVA
                     HTGIVLSAMQLVTITYAGYAAAPDVVEFGDALVASSWYRTIGAEYRVAPASHRQQVVL
                     GAPPAMLDRDHIAAQILDLVTGGGVVPPDRASNQLLYLLYVPPSVALGSDLVGVAGYH
                     EMLTLGGVRFPIAVVIDDGSGLATTTTQAAHQVIDAATNPYLPPNDGYYADPPQTDPW
                     SLLRREVADLCEGEDVVIEGRFAFPRVYSNTAAVASMTPCTPVRPGDVYYDVTAEPSQ
                     IQTVKPGNSVKYRLTGWSTGPLPAWSVRVRAAASSMLSQDEMRPELSSDTINNGEMVT
                     LTLHAPPEAAPGTTGGVAVLSGANQHLWAVGFIVRP"
     gene            complement(3847..5190)
                     /locus_tag="VF516_34275"
     CDS             complement(3847..5190)
                     /locus_tag="VF516_34275"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112865.1"
                     /translation="MACGGGAAYQNIPSAPEAPAPKDVPEPEPAPPPKSLFDRLGGEP
                     AITAVVGEFVGRTTTDPRIKERFFNTDADNLKRLLVEFVCQATGGPCKYSGRDMATSH
                     AGMDLVDDEFTALVEDLAGALDKFNVPDKEKGELLGALGPLKPQIVASPDKLRPIDDA
                     RLASAQKLAASIKDRTAADLLGLAITAGKRGQRSYAEQLFSRAEMVTGPRQLAPIAAV
                     FRAGAPQRVATALKKLPADTAPQPMTVGGSETDAPDKRPAAGSLHGAIVIDGKPPTGL
                     GVVMLWPERGARKRVAKQRIIEQRNKTFAPHVMAVPVGSTVSFPNFDPIFHNVFSLSK
                     AKPFDLGMYKNGETREVKLDKPGIVRLGCNLHANMSAYLVVVDAPHYVIVNSDGTYSF
                     KSLAPGKYRVQAWNEQSGEPLTTSVTIKAGANENNLELAGGGQAISPDKFGNARQ"
     gene            complement(5237..>5885)
                     /locus_tag="VF516_34280"
     CDS             complement(5237..>5885)
                     /locus_tag="VF516_34280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112866.1"
                     /translation="ANLIDAEARAAQLRASGIAGMPMLRAAIETDAATLHDMAGSDFI
                     FTPMPGEALELFQLSDRGTPASMLRIPETSPPLPPVLGNQTRITHDGRKITIIAGAPV
                     SKQRAGIGGSVAIAVPIDLAPVHKRIAEHVRSAALVGLGEPLTLVGGSGAGRAVSIPL
                     PLGSNLQTGPLALAAVVPPPGIRPELRIVGLGCWGLAAVLLALFAANLLRGRKPE"
BASE COUNT          855 a         1938 c         2160 g          932 t
ORIGIN      
        1 gtgcgcttga tgtcggagtg cgtgatgttc gccggtccgg tcacccgctt gatctgggcc
       61 aggttcccca gggtctggac cttgttcgtg gcggtcgaga tcggggtcgc gcgcagcgcg
      121 tcgatcgagt cgatcgcgta ctgcggggtc tggaccgcga ccgtgtactg gacgccgcgg
      181 cggtcgatcc agaacgacgg cgcgacgtca ctgctcgatg ccagcgagac cagcaggtcg
      241 ttcgcgacgt cgcgctcggt caggccaacc tgctgggcct cgacccggtc gatcgcgacc
      301 tggagctggg gcacccgcga ctcctgggcg aggtggacgt cgaccgcgcc cgggaccttc
      361 gcgacctcgg cggcgagctt ctcggcgagc gcgagcgtct ggtcctcgct gccgatcggc
      421 ccgacgacct ggagatcgat cggcgcggcg agcccgaagt tgaggacctg ggtcgagatg
      481 tcgggcgcga ggaagaagaa cgtggtctcg gggtagacgc ggtgcagccg ctcgcgcagc
      541 gccttgaggt agtcctcggt cgggcggtgg ccgggcttga gcgcgatcat gatctggctg
      601 tcggccggcg acaccgcggc gccctcgctg agcgacaggt tgatccccga gtacggcgtg
      661 ccgagcacgt cgagcatggt ctcgatctcg ccgggcggga tcaccccgcg gatcgtctgc
      721 tcgatcgccc cgatccgctt ctcgctctcc tcgagccggg tccccggcac atcgcgcacg
      781 tggagcttga tcagcccggc gtcgacggtc gggaagaagt cgcggccgac caggctgatc
      841 agcgcgagcg agcccgcgat gatgatcgcg aacccgccga ccacgaaccc gcggtgctgc
      901 agcgcccacg ccagcctgcg gccgtagctg tcgcgcagcg ccgagaaccc gcgctcgaac
      961 gccacggtga accggttcgg cccacccgac tcgtgcttga gcaggtagtg gaccatggtc
     1021 ggcaccaggg ttcgcgacag caggtacgac atcagcatcg cgaacaccac cgcgagcgcc
     1081 atcggcacga acagcgactt ggcggcgccg gtcaggaatg cgatcggcgc gaacacgatg
     1141 cagatgcaca gggtcgacac gaacgccggc acggcgatct gctgcgcgcc gtcgatgatc
     1201 gcgcgggtga acggcttgcg ctggtgcatg ttgcggtgga tgttctcgat cgcgacggtg
     1261 gcgtcgtcga ccaggatgcc gaccgcgagc gcgaggccgc cgagcgtcat cacgttgagg
     1321 gtctcgccga gcgcgtgcag gacgacgatc gagaacagga tcgacagcgg gatcgagatc
     1381 acgacggtca gggtcgagcg ccagctgccg aggaacatca ggatcatcag cccggtcagg
     1441 cccgcggcga tcagcgcctc gcggatcacc ccgtcgatcg acgcgcgcac gaagatcgac
     1501 tgatcgaaca gcatcttgac ctggaggtgg ccctgcgcct ccttggggag gcgatcgagc
     1561 gcgcgcggga tcgcctcgcg gatccgctcg ttgacctcga gcgtcgaggc gtcgccgttc
     1621 ttgaggatcg tcatcagcac cgagcgccgg ccctcgacgt ggaccatgtt ggtctgcggc
     1681 gcgttgccgt cgcggacgtt ggcgacgtcg cggatgtaca cggtgcggcc gtcgaccgtc
     1741 ttgatcggca ggccggcgag ctcggcgagc gtctcggggg tcgacgcgac gaagatcggg
     1801 tactcgttgg ttccgagctt ggcggtgccg gacggcagca cgacgttctg caccgcgagc
     1861 gcgttctgga cgtcgcgcgg cgatagcccg agcgcgtgca ggcgctgggg atcgatgtcg
     1921 accatgatct ggcgctgctt gccgccgtac gggtacggga tctgcgcgcc cgggatcgtc
     1981 gcgatctcgg cgcggatgaa gttgatcccg tagtcgaaca gctgctgctc ggacagcgag
     2041 tcgctctcca tcgcgatctg catgatcggg acggcggtcg cgctgtactg catgatcagc
     2101 ggcggcacca tgccgggcgg catcgagcgg acggcggtct gggagatcgc ggtggtctgc
     2161 gcgatcgcct gcgagacgtt ggcgccgggc tggaggtaga tcttgacgat cgcctggccg
     2221 gtcacggaca ggctctccat gtggtcgata tcgctgacga tcgtggtgag gaagcgctcg
     2281 aaattattga cgatccgctt ctccacgtcc tcgggctgta gcccgctgta acggaacagc
     2341 accgagatca ccgggatatt gatgctcggg aagatgtcgg tcggggtccg gaggtacgac
     2401 agcacccctc cgatcgcgat caacatggcc atgacgacga aggtgtaggg gcgccggagc
     2461 gccgttcgga tcagccacat cgacgggtac ccagtgtgcc gccattcctc acgctgtcca
     2521 gagacaggcg gcatgtcgca ccggtgggtt aacagttcgt cgtacgaaca caacaatcgt
     2581 aaaagtcaac cctgaatgtc ggagcaggtc gccggggtgc acatgtgcag ccgacgcggc
     2641 cctcgcagga gcttgcacat cggccgtcgg ttcgagtgcc tgttgaaatc gccggcgagc
     2701 tggtgtgtgt ttgggctatg cggcgattcg cgatggtgct gtcggcttgc ctgtgggcat
     2761 gcggcgacaa tcagaccggc ccgccggatc tccagcccga cgccgggttc catcccgcgc
     2821 cgcacgcacc gctgccgacg gtggtcgccc ataccggcat cgtgctgtcg gcgatgcagc
     2881 tcgtcaccat cacctacgcg ggttacgccg cggcgcccga cgtcgtcgag ttcggtgatg
     2941 ccctggtcgc gtcgagctgg taccggacga tcggcgccga gtaccgcgtg gcaccggcga
     3001 gccatcgcca gcaggtcgtg ctcggcgcgc caccggcgat gctcgaccgc gaccacatcg
     3061 cggcgcagat cctcgatctc gtcaccggcg gcggggtggt cccgcccgac cgggccagca
     3121 accagctcct gtacctgctc tacgtcccgc cctcggtcgc gctcggctcc gacctggtcg
     3181 gcgtcgccgg ctaccacgag atgctcacgc tcggcggcgt gcggttcccg atcgcggtcg
     3241 tgatcgacga cggcagcggt ctcgcgacga ccaccaccca ggcggcgcac caggtgatcg
     3301 acgcggcgac caacccgtac ctgccgccca acgacggcta ctacgccgac ccgccgcaga
     3361 ccgatccatg gagcctgctg cgccgcgagg tcgccgatct gtgcgagggc gaggacgtgg
     3421 tgatcgaggg ccggttcgcg ttcccgcgcg tgtactccaa caccgccgcg gtcgccagca
     3481 tgacgccgtg cacgccggtc cggcccggcg acgtctacta cgatgtcacc gccgagccgt
     3541 cgcagatcca gaccgtcaag cccggcaact cggtcaagta ccggctcacc ggctggtcga
     3601 ccggcccgct gcccgcctgg agcgtccggg tccgcgcggc cgcgtcctcg atgctcagcc
     3661 aggacgagat gcgccccgag ctcagcagcg acacgatcaa caacggcgag atggtgacgc
     3721 tcacgctgca cgcgccgccc gaggccgcgc cgggtaccac cggcggcgtc gcggtgctgt
     3781 cgggggccaa ccagcacctg tgggcggtcg gcttcatcgt gcgcccctga ccgcgccgca
     3841 cgccggctac tggcgcgcgt tgccgaactt gtcggggctg atcgcctggc cgccgccggc
     3901 gagctcgagg ttgttctcgt tggcgccggc cttgatcgtg acgctggtgg tcagcggctc
     3961 gccgctctgc tcgttccacg cctggacgcg gtacttgccg ggcgcgaggc tcttgaacga
     4021 gtaggtgccg tcgctgttga cgatgacgta gtggggcgca tcgacgacga ccaggtacgc
     4081 gctcatgttc gcgtggaggt tgcagccgag ccggacgatg cccggcttgt cgagcttgac
     4141 ctcgcgggtc tcgccgttct tgtacatgcc gaggtcgaac ggcttggcct tggacagcga
     4201 gaacacgttg tggaagatcg ggtcgaagtt ggggaacgag accgtcgagc cgaccgggac
     4261 ggccatgacg tgcggggcga acgtcttgtt gcgctgctcg atgatgcgct gcttggcgac
     4321 gcgcttcctg gcgccgcgct cgggccacag catgacgacg ccgaggccgg tcggcggctt
     4381 gccgtcgatc acgatcgcgc cgtgcagcga gccggcggcc gggcgcttgt cgggggcgtc
     4441 ggtctccgag ccgccgacgg tcatcggctg cggcgcggtg tcggccggca gcttcttgag
     4501 cgcggtggcg acgcgctgcg gggcgccggc gcggaacacg gcggcgatcg gggcgagctg
     4561 gcgcgggccg gtgaccatct cggcccggct gaacagctgc tcggcgtagg accgctggcc
     4621 gcgcttgccg gcggtgatcg cgagtccgag caggtcggcg gcggtcctgt ccttgatcga
     4681 cgccgcgagc ttctgggcgg aggcgagcct ggcgtcgtcg atcggcctca gcttgtcggg
     4741 cgaggcgacg atctgcggct tgagcgggcc gagcgcgccg agcagctcgc ccttctcctt
     4801 gtcggggacg ttgaacttgt cgagcgcgcc ggcgaggtcc tcgaccaggg cggtgaactc
     4861 gtcgtcgacc aggtccatgc cggcgtgcga ggtcgccatg tcgcggccgc tgtacttgca
     4921 cgggccgccg gtggcctggc agacgaactc gacgagcagg cgcttgaggt tgtcggcgtc
     4981 ggtgttgaag aaccgctcct tgatccgcgg gtcggtggtg gtgcggccga cgaactcgcc
     5041 gaccaccgcg gtgatcgccg gctcgccgcc gaggcggtcg aacaggctct tgggcggggg
     5101 ggccggctcg ggctcgggga cgtccttggg ggccggcgcc tcgggggcgg atgggatgtt
     5161 ttgataggcc gcccccccac cgcaggccat ggacagacag atcgatagtg cggtcgctcg
     5221 aatcatgttc acggctctac tccggtttgc gaccgcggag caagttcgcc gcaaacaaag
     5281 ccagcagcac ggcggccagg ccccagcagc ccaggccgac gatccggagc tcgggtcgga
     5341 tgcccggcgg cgggacgacg gcggcgagcg cgagcgggcc ggtctggagg ttggagccga
     5401 gcgggagcgg gatggagacc gcgcggccgg cgccggagcc gccgaccagc gtcagcggct
     5461 cgccgaggcc gaccagcgcc gccgaccgga cgtgctcggc gatccgcttg tgcaccggcg
     5521 cgagatcgat cggcaccgcg atcgcgacgg agccgccgat gccggcgcgc tgcttggaga
     5581 ccggcgcacc ggcgatgatc gtgatcttgc ggccgtcgtg ggtgatccgg gtctggttgc
     5641 cgagcaccgg cgggagcggc gggctggtct cggggatccg cagcatcgac gccggggtgc
     5701 cgcggtccga gagctggaac agctcgagcg cctcgccggg catcggcgtg aagatgaagt
     5761 cgctgccggc catgtcgtgg agcgtcgcgg cgtcggtctc gatcgcggcg cgcagcatcg
     5821 gcatcccggc gatgccgctg gcgcggagct gggcggcgcg cgcctcggcg tcgatcaggt
     5881 tggcg
//
LOCUS       DASVOR010001843         2262 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_487078,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001843 DASVOR010000000
VERSION     DASVOR010001843.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487078"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..500
                     /locus_tag="VF516_34285"
     CDS             <1..500
                     /locus_tag="VF516_34285"
                     /inference="COORDINATES: protein motif:HMM:NF013056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEX8112867.1"
                     /translation="ARYLDERGDFPRPGAVLLIDSLAAAAGGAVGASSATTYIESASG
                     AAAGGRTGLTSVVTGLLFALCLFLSPLPGVVPPQATGAILVLVGFLMMREVAAIRWDA
                     ADEAIPAFLTLTVMPFTYSITNGIGAGFLAFVGLKLLAGRIREVHPLMLGASVAFLVY
                     FALGG"
     gene            587..1093
                     /locus_tag="VF516_34290"
     CDS             587..1093
                     /locus_tag="VF516_34290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112868.1"
                     /translation="MCAARGASAEDAVPEAKDRYVAFALSAGGTGLSIGLVALGGKTG
                     NNTLATAGLLSSLVTPAAGEIYADKLVTWGMGIRLVSAGVLVVGYAEAFRCLFASEPC
                     STDPSAAGTMIAVGALGYATGVVYDIAAAGSVVDDYNRRLRLRVTPTVIPTASSSPAV
                     GLGIGGSF"
     gene            complement(1107..1868)
                     /locus_tag="VF516_34295"
     CDS             complement(1107..1868)
                     /locus_tag="VF516_34295"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112869.1"
                     /translation="MGATQSRDVRAGAEHAGDPRSADPGGDGDASHGDESPSELPPLP
                     GRFAAGTTPIATHTGGFFSQLRYLGQLDLTYLACEGDGELVLVDQHAAHERVELARLR
                     ERHDGDARVAVQNMLFPVTLDATPAQLALVARVGDLLAQVGFEVEPFGKATLAVKAVP
                     AGIRHGDPAQLLRRLLHEWAEAGAPSEAERLEALLGTIACHSVVRAGDRLTPGEAETL
                     LRSLDGIDLSLPAPHGRAVLLRLPLSEIGRRFGRS"
BASE COUNT          284 a          821 c          827 g          330 t
ORIGIN      
        1 aggcgcgata cctcgacgag cgcggcgact tcccgcgccc gggcgccgtg ctgctgatcg
       61 actctctcgc cgccgcggcc ggcggcgcgg tcggcgccag ctcggccacg acctacatcg
      121 agagcgcctc gggcgccgcg gccggcgggc gcaccgggct gacctcggtc gtcaccggcc
      181 tcctgttcgc gctgtgcctg ttcctgtcgc cgctgccggg cgtggtgccg ccgcaggcca
      241 ccggcgcgat cctggtgctg gtcggcttcc tgatgatgcg cgaggtcgcc gcgatccggt
      301 gggatgctgc cgacgaagcc atcccggcgt tcctgacgct cacggtgatg ccgttcacct
      361 actcgatcac caacggcatc ggcgccggct tcctcgcgtt cgtcgggctg aagctgctcg
      421 ccggccggat ccgcgaggtc catccgctca tgctcggggc gtcggtggcg ttcctcgtgt
      481 acttcgcgct ggggggatga cagcggccgg aacgctggga ccatgaaatg catcggcggc
      541 ggatatgccg aagatgctgg tcgcggttgc cgcaggtttt gcgctgatgt gcgctgcgcg
      601 tggcgcatct gcagaggatg cggtccccga ggcaaaggac cgctacgttg cgtttgcgct
      661 gtccgccggc gggaccgggc tctcgatcgg gctggtcgcg ctcggcggca agaccggcaa
      721 caacacgctc gccacggcgg gcctgctgag ctcgctggtc acgccggccg cgggcgagat
      781 ctacgccgac aagctggtga cctggggcat gggcattcgc ctcgtgtcgg cgggcgtgct
      841 ggtcgtcggc tacgccgagg ccttccggtg cctgttcgcc agcgagccct gcagcaccga
      901 cccatcggcg gccgggacga tgatcgcggt gggcgccctc gggtacgcga ccggcgtcgt
      961 ctacgacatc gccgccgcgg gcagcgtggt cgacgactac aatcgacggc tgcgcctccg
     1021 cgtgacaccg acggtcatcc cgacggcgtc gtcgagcccc gcggtcggcc tgggcatcgg
     1081 cggctcgttc tagaagccgt gccttcctaa ctccggccga accggcggcc gatctcggac
     1141 agcggcaggc gcaacagcac cgcgcggcca tgcggcgcgg gcagcgacag gtcgatgccg
     1201 tcgagcgagc gcagcagggt ctcggcctcg ccaggggtga gccggtcgcc ggcgcgcacc
     1261 accgagtgac aggcgatcgt gccgagcagc gcctcgagcc gctcggcttc gctgggcgcg
     1321 ccggcctccg cccattcgtg gagcaggcgg cgcaagagct gcgccggatc gccgtggcgg
     1381 atgccggccg ggaccgcctt gacggcgagc gtggccttgc cgaacggctc gacctcgaag
     1441 ccgacctgcg cgagcaggtc gccgacgcgg gcgaccaggg cgagctgcgc cggggtggcg
     1501 tcgagggtga ccgggaagag catgttctgg accgcgaccc gcgcgtcgcc gtcgtggcgc
     1561 tcgcgcagcc gggcgagctc gacccgctcg tgcgcggcgt gctggtcgac caggacgagc
     1621 tcgccgtcgc cctcgcacgc caggtaggtc aggtcgagct ggccgaggta gcgcagctgc
     1681 gagaagaagc cgccggtgtg cgtggcaatg ggcgtggtac cggcggcgaa ccggcccggc
     1741 agcggcggca gctccgacgg ggattcgtcg ccgtgcgagg catcgccatc gcccccgggg
     1801 tccgcggagc gtggatcccc tgcgtgctcg gcaccggcgc gaacgtcgcg ggactgcgtg
     1861 gcccccacag gggggtcggg aacacgctcc actccctcgt ccgcgacgcg tggatccctt
     1921 gcgtacccac cgcgcgcgga aacatctcgg ccctgcgcgg cgcccacgtg ggggtccgaa
     1981 atgcggtccg ctccctcgtc cgcgaggcgt ggatcgcttg ggtacccacc gccaccgcga
     2041 acgtcgtggc ccgggccggc ccccacatgg gggtccactc cctcatccgc gaggcgtgga
     2101 tcccttgcgt acccaccgcg cacggaaaca tctcggccct gcgccgcccc cacgtggggg
     2161 tctgaagcac ggtccactcc ctcgtccgcg acgcgtggat cccttgcgta cccaccgcgc
     2221 acggaaacat ctcggccctg cgccgccccc acgtgggggt cc
//
LOCUS       DASVOR010001844         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_165252,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001844 DASVOR010000000
VERSION     DASVOR010001844.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165252"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VF516_34300"
     CDS             <1..526
                     /locus_tag="VF516_34300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112870.1"
                     /translation="RAAHYGVTVDRNAWRLVGLVHLAETREQAYRDVEHGILQWFDYF
                     QNTAAFPQMAVGDGTSPRQCIDFVNQSGLGAIGTPDDAIAQIQRLAAQSSGGFGCFLH
                     LAHDWAAPAARFRSYELFARHVAPHFQGQYQATTDARTRARAARPELAQDNLKAVAQS
                     TARYQAELEAKKRS"
     gene            656..2962
                     /locus_tag="VF516_34305"
     CDS             656..2962
                     /locus_tag="VF516_34305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112871.1"
                     /translation="MRTWVMALMLLVSLGLSSAAADGGASESNVSFYVSEQFKAVAKR
                     LVTQYIGEKLADACPEGGTMCEAIIARIPDAVQAAVDGDTDRLRAILTSFFVDSSIGG
                     AVSLAVGGLATLAPESTHDARLERLGKLVRPLLGCLVKIAQDHRSGCSLGATFGAELK
                     AINADLHIILQGTLDEAAFDRLVADPLHPQPADVVRILEAVASSSYVERPDLRQYLLA
                     IEIALRNGTEAGVYAFVESLLGANISAVTLLRDAPADPAKAVPLLRNVPTDPAKFPVL
                     SADTETIDGKEISYQTAFTACGQSQLWSDWVARRAAIEANLRRAVWLQQSVRAALDAL
                     APITGASCASPKADAINVKALMILRKHALYLKGALDMFELRNRGAIVLAIAAVIDLVR
                     TSDKDQFERHTRTAVAGAVDRLGWQLCVDAQDPDLCVKQAQAGQPVCQTALVSAVLRG
                     TDPRKVAPCLNLNTKRVCKTIVSTNDQQPDSCVFPEIALVESKPEEFRKALLETVLTK
                     YVEPYREKLVNDASAATKIVDAAIRCLEYLRAGDSTAARKAIARVAIEELLDHLKEVI
                     AEQLHADSATCEDSTHSVSLFSGLGAKCAVTVLIEAAYYPIADMIWDGGIDASKASQI
                     AESAYRSVLQSKLLAGSPILVNVGLGANAIWGADSVWGKDGYKALTVVDKFGLVFYRR
                     QFSKETFEIGPFVGGFLDALVRTVNGSNRFFWLAGVTAGFPRIRGYNLGIELHAAYAL
                     PFAFESRNDVGYALGGVLVIPWDYWKEK"
BASE COUNT          572 a         1179 c         1147 g          538 t
ORIGIN      
        1 gcgcgcggcg cactacggcg tcaccgtcga ccgcaacgcg tggcggctgg tcggcctggt
       61 ccacctcgcc gagacccgcg agcaggccta ccgggacgtc gagcacggca tcctccagtg
      121 gttcgactac ttccagaaca cggccgcgtt cccccagatg gcggtcggcg acggcacctc
      181 tccgcgccag tgcatcgact tcgtcaacca gtccgggctc ggcgcgatcg gcacgccgga
      241 cgacgcgatc gcccagatcc agcggctcgc ggcgcagtcg agcggcgggt tcggctgctt
      301 cctgcacctc gcgcacgact gggcggcccc ggcggcccgg ttccgcagct acgagctgtt
      361 cgcccgccac gtcgcgccgc acttccaggg ccagtaccag gcgaccaccg acgcccggac
      421 ccgcgcccgc gcggcgcgcc ccgagctcgc ccaggacaac ctcaaggcgg tcgcgcaatc
      481 gaccgcgcgc taccaggcgg agctcgaggc caagaagcgg agctagcggc ggtggtgccg
      541 gtggtcgcga tgggcaaccc gggggatacg ctccggcggc ctgccagccg cattgcagca
      601 aaatgcggca tagctcacga tgtgatggct catccacatc cactatcgtg gctccatgcg
      661 cacgtgggtg atggcgttga tgctactcgt cagtctgggg ctgtcctcgg cagctgccga
      721 cggcggtgca agtgagtcga atgtctcgtt ctacgtgagc gagcaattca aggcggtcgc
      781 gaagcggctc gtcacccagt acatcggaga gaaactcgcc gacgcctgtc ctgagggcgg
      841 cacgatgtgc gaggcgatca tcgcgcggat cccggatgcg gtccaggccg cggtcgatgg
      901 ggacacggac cgcctgcgcg ccatcctcac ctcgttcttc gtcgacagct cgatcggtgg
      961 tgccgtgtca ctggccgtcg gggggctcgc caccctcgcg cccgagtcaa cgcacgatgc
     1021 caggctcgag cgcctcggga agcttgtccg cccgctcctc gggtgtctcg tcaagatcgc
     1081 acaggaccac cgctccggct gctcgctggg cgcgaccttc ggcgccgagc tcaaggccat
     1141 caatgcggat ctccatatca tcctgcaggg aaccctggac gaggccgcgt tcgaccggct
     1201 cgttgcagat ccgctgcacc cacagcccgc cgacgtcgtg cggatcctcg aagcagtggc
     1261 gtcctcctcc tacgtcgagc gccccgatct ccggcagtac ttgctcgcga tcgagatcgc
     1321 cttgcgcaac ggtacagagg ccggtgtgta tgcgttcgtc gagagcttgc tgggcgcgaa
     1381 catctccgcc gtgacgctgc tgcgcgacgc gcctgccgat ccggcgaagg ccgtgccgct
     1441 gctgcgcaac gtgcctaccg acccggcgaa gttccccgtg ctgtctgccg acacggagac
     1501 gatcgatggc aaggagatca gctaccagac ggccttcacg gcctgcggac agtcacagct
     1561 gtggagcgac tgggtggccc gcagagcggc catcgaggcc aacctgcggc gggccgtctg
     1621 gctccagcag agtgtgcgtg ctgcgctgga tgcgctggcc ccgatcacgg gggcatcctg
     1681 tgcgtcgccg aaagccgacg cgatcaacgt gaaggccctg atgatcctgc gcaagcacgc
     1741 actgtacctg aagggcgccc tggacatgtt cgaactgcgg aaccgcgggg ccatcgtcct
     1801 cgcgatcgcc gccgtgatcg atctcgttcg cacctccgac aaggatcagt tcgagcgaca
     1861 taccaggacc gcggtcgccg gcgcagtcga tcgcctgggc tggcagctct gcgtcgacgc
     1921 ccaggatccc gatctctgtg tcaagcaagc ccaggcaggt cagcccgtgt gtcagaccgc
     1981 gctggtgtcc gccgttctcc ggggcaccga tccccggaag gtcgccccgt gcttgaacct
     2041 caacacgaag cgcgtctgca agacgatcgt gtccacgaac gaccagcagc ccgactcctg
     2101 cgtgttcccc gagatcgcgc tcgtcgagtc caagcccgag gagttccgca aggcgcttct
     2161 cgagaccgtc ctgacgaagt acgtggagcc gtatcgcgag aagctcgtca atgacgcgag
     2221 cgccgcgacc aagatcgtcg acgcagcgat ccgttgcctg gagtatctca gggcgggcga
     2281 cagcacggcc gcgcgcaagg ccatcgcacg cgtggcgatc gaggagctgc tcgaccacct
     2341 caaggaggtc atcgcggagc agctgcacgc cgactccgcc acctgcgagg acagcacgca
     2401 cagcgtctcg ctgttcagcg gcctcggcgc gaagtgtgcg gtcaccgtgc tgatcgaggc
     2461 cgcgtactac ccgatcgccg acatgatctg ggacggtgga atcgatgcca gcaaagcatc
     2521 ccagatcgcg gagtccgcct accgcagcgt cctgcagtcc aagctgctcg ccggctcacc
     2581 gatcctcgtg aacgtcggcc tcggagccaa cgcgatctgg ggcgccgaca gcgtgtgggg
     2641 caaggacggg tacaaggcgc tcaccgtcgt ggacaagttc ggtctcgtgt tttaccggcg
     2701 ccagttctcg aaggagacgt tcgagatcgg gccgttcgtc ggcgggtttc tcgacgcgct
     2761 ggtccggacg gtgaacggca gcaaccgctt cttctggctg gcgggcgtca cggcggggtt
     2821 cccgcgcatc cgcggctaca acctgggcat cgagctgcat gccgcgtacg cgctgccgtt
     2881 cgcgttcgag tctcgcaacg acgtcggcta cgccctcggt ggcgtgctcg tgatcccctg
     2941 ggattactgg aaggagaaat agcgatgggg aaggcaaaga ccaagacaac gagaagtccg
     3001 agcaagcaga ccccgagccc ggggaaggcg cggcatccca agtactgctg gaattgcggg
     3061 cagctcgtcg ccgacctggc tgcggagcgc tccgccaacc gcagtttcgc atcgtgctgc
     3121 gagtgcggat ttccactcga tccgcaaggg cagtgtcaga accaaggctg tccattctat
     3181 ggcgtgcgcc ctgcgtgcta gcagcccggc tcgctgaaga gggcggaagg ccgccctcgc
     3241 gcgggtcgcc gacgcgacca cgcacgctga gctcgcgtcc gagctagcgg cgggtcagcg
     3301 cggcgacgcc gtcgagcagg cgatcgacct cgtcggcgga cgtgtagcag gcgcagccgg
     3361 cgcgcacgac gccgtcgtcg gcgtggccga gccgggtgac cacggtctgg gcgtagacgt
     3421 cgccgtgcga gacgaa
//
LOCUS       DASVOR010001845         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_48956,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001845 DASVOR010000000
VERSION     DASVOR010001845.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48956"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VF516_34310"
     CDS             <1..327
                     /locus_tag="VF516_34310"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112872.1"
                     /translation="PLAAAYDLIVLDAPCSGLGVLRRHPDAKWRLVPGDVPRLAELQC
                     RLLDAMLPRLAPGGALVYSVCTFTRAEGPDQIAALVARTGLRLVEEHRTWPPDADAFH
                     IARLER"
     gene            complement(343..>1504)
                     /locus_tag="VF516_34315"
     CDS             complement(343..>1504)
                     /locus_tag="VF516_34315"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8112873.1"
                     /translation="DDPPLAGVAELEAAQRLEPGELATSPGSWDSQVRERGLPDAGMI
                     VRGVSDAAMLLARMFAQGATGRVGFRRDEVETVVYFDRGRPVFASSNEPRDRMGELLL
                     REGKITASQLERCQAVVAESGRRMGEILVDFGYLKRRELLPAVRRHVEDLVYSLFGWE
                     RGTYTITLDAEPSAERIRLSRHPAALILEGIRRKLDRTSLERLVGSPSTVIEVRDRDR
                     LGGIVNAGDLAAEERAALAMFDGQADLAQVARSAGVDIADVLPLAWALCVLGLATARR
                     ADAEIPDEATALVGETDLAIDRERVRARWQLVTEADYFALIGVRRDATGFEIRRAYQS
                     ARRDFAADCFPTELRRELARELDDIASVLDEAFRVLRDDRLRQTYLANLVES"
BASE COUNT          182 a          572 c          563 g          187 t
ORIGIN      
        1 ccgctcgcgg cggcgtacga cctgatcgtg ctcgacgcgc cgtgctccgg cctcggcgtg
       61 ctgcggcgcc accccgacgc caagtggcgg ctcgtgcccg gcgacgtgcc gcggctggcc
      121 gagctccagt gccggctgct cgacgcgatg ctgccgcggc tcgcgcccgg cggggcgctg
      181 gtgtactcgg tgtgcacgtt cacccgcgcc gagggccccg accagatcgc cgcgctggtc
      241 gcgcgcaccg ggctgcggct cgtcgaggag caccggacct ggccgccgga cgccgacgcg
      301 ttccacatcg cgcggctcga gcgctgagcc ggacccggct ggtcagctct cgaccaggtt
      361 cgccaggtag gtctggcgca gccggtcgtc gcgcagcacg cggaacgcct cgtcgagcac
      421 gctggcgatg tcgtcgagct cgcgcgccag ctcgcggcgc agctcggtcg ggaagcagtc
      481 ggcggcgaag tcgcggcgcg ccgactggta ggcgcgccgg atctcgaagc cggtcgcgtc
      541 gcggcgcacc ccgatcagcg cgaagtagtc ggcctcggtg accagctgcc agcgcgcgcg
      601 cacccgctcg cggtcgatcg cgaggtcggt ctcgccgacc agggcggtgg cctcgtcggg
      661 gatctcggcg tcggcgcgcc gcgcggtcgc gaggccgagc acgcacagcg cccacgccag
      721 cggcagcacg tcggcgatgt cgaccccggc gctgcgcgcg acctgcgcca ggtccgcctg
      781 gccgtcgaac atggcgagcg cggcgcgctc ctcggccgcg aggtcgccgg cgttgacgat
      841 gccgccgagc cgatcgcggt cgcggacctc gatcacggtc gacggcgagc cgaccaggcg
      901 ctccaggctg gtgcggtcga gcttgcgccg gatgccctcg aggatcagcg ccgcggggtg
      961 gcgcgacagc cggatccgct cggcggacgg ctcggcgtcg agcgtgatcg tgtaggtgcc
     1021 gcgctcccag ccgaacagcg agtacaccag gtcctcgacg tggcgccgca ccgcgggcag
     1081 cagctcgcgg cgcttgaggt acccgaagtc gaccaggatc tcgcccatcc gccgcccgga
     1141 ctcggcgacc acggcctggc agcgctcgag ctgcgacgcg gtgatcttgc cctcgcgcag
     1201 cagcagctcg cccatccggt cgcgcggctc gttcgacgat gcgaacaccg gccggccgcg
     1261 gtcgaagtac acgacggtct cgacctcgtc gcggcggaac ccgacccggc cggtcgcgcc
     1321 ctgcgcgaac atccgggcga gcagcatcgc ggcgtccgac acgccgcgca cgatcatgcc
     1381 ggcgtcgggc agcccgcgct cgcggacctg gctgtcccag gatcccgggg aggtcgcgag
     1441 ctcgccgggc tccaggcgct gtgccgcctc gagctcggcc acgccggcga gcggcggatc
     1501 gtcg
//
LOCUS       DASVOR010001846         2268 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_48958,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001846 DASVOR010000000
VERSION     DASVOR010001846.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48958"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1547
                     /locus_tag="VF516_34320"
     CDS             <1..1547
                     /locus_tag="VF516_34320"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8112874.1"
                     /translation="DLAALIARDVLDALDYAHGALDASGRPLGIIHRDISPGNVLLSW
                     RGDVMLTDFGIARAAERRHKTEAGTLKGKYGYMSPEQVSGSEVDARSDLFSVGILLAE
                     MVMARRLFTSTNDLDILLMVRDARLDRLHKYASEFPVELRVLITRALQRRPEDRWQSA
                     AQFRDALDEWLRRTTRASARQLAALLGQVINAPTADLHGRGEGVVADESDDDSALSGP
                     LTRMSQAHAVAEAARARAAFISGEAIPSGTTGDIGSSGIVQITRDEDDVAPEDVESPS
                     GPREGGDIAQHPVLRVVYLLAKSKGTGLLALEGRAGVLKEAYLVDGQPQFVSSNVESE
                     RLGNYLIQHGALTPQALQRAMSVVHHFGGRLADTLVGLDILDPLEAYRLLAKQVAAKL
                     MEAFSWQKGRYTWTPRHPNPWKTRSLHLDAFRVIGAGAAQLGEPLIEDWLSANLKGFV
                     VREPIQDDEMAQFGLGDALLRVYSLLDGRTRLGELAHKVRSPEARMNLLRLTYLLVQT
                     DLARLS"
     gene            1544..>2268
                     /locus_tag="VF516_34325"
     CDS             1544..>2268
                     /locus_tag="VF516_34325"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8112875.1"
                     /translation="MTAILTIQGLEKRFGTRRVLAGASFAVHERDRIGMIGANGAGKS
                     TLLETVVHRAMGGDDPTLEPDAGLVTWKRDLALEYVAQEPVLDLELAVGAALARDGVP
                     DHEIATIAAALELPPADQRIATLSLGERRRVALARALLGKADVLALDEPTNHLDATTV
                     EWLETRLAAWPGALIVVTHDRYFLDRVATRILEVDRGKVYSYDGDYAEFILQQAARLA
                     AEAEVEYQRASFIRREIDWIRRGP"
BASE COUNT          321 a          853 c          796 g          298 t
ORIGIN      
        1 ccgacctcgc ggcgctgatc gcgcgcgacg tgctcgacgc gctcgactac gcccacggcg
       61 cgctcgacgc gtcgggccgg ccgctcggca tcatccaccg cgacatctcg cccggcaacg
      121 tgctgctgtc gtggcgcggc gacgtcatgc tgaccgactt cggcatcgcg cgcgccgccg
      181 agcgccggca caagaccgag gccggcacgc tcaagggcaa gtacggctac atgtctcccg
      241 agcaggtctc gggcagcgag gtcgacgcgc gctccgacct gttctcggtc ggcatcctgc
      301 tcgccgagat ggtgatggcg cgccggctgt tcacgtcgac caacgacctc gacatcctgt
      361 tgatggtgcg cgacgcgcgg ctcgaccggc tgcacaagta cgcgtcggag ttcccggtcg
      421 agctccgcgt gctgatcacc agggcgctgc agcgccgccc cgaggaccgc tggcagagcg
      481 ccgcgcagtt ccgcgacgcg ctcgacgagt ggctgcgccg caccacccgg gcgtcggcgc
      541 gccagctcgc cgcgctgctc ggccaggtga tcaacgcacc cacggcggat ctacatggcc
      601 gtggcgaagg cgtggtcgcc gacgagagcg acgacgacag cgcgctgtcc gggccgctga
      661 cccggatgag ccaggcccac gccgtggccg aggcggcgcg ggcgcgcgcc gcgttcatct
      721 ccggcgaggc gatcccgagc ggcaccaccg gcgacatcgg ctcgagcggc atcgtccaga
      781 tcacgcgcga cgaggacgac gtcgcgcccg aggacgtcga gagcccgtcg gggccgcgcg
      841 agggcggcga catcgcccag cacccggtgc tgcgcgtggt gtacctgctc gcgaagtcca
      901 agggcaccgg cctgctcgcg ctggagggcc gggccggcgt gctcaaggag gcctacctgg
      961 tcgacggcca gccccagttc gtcagctcca acgtcgagag cgagcggctc ggcaattacc
     1021 tgatccagca cggcgcgctg accccgcagg cgctgcagcg cgcgatgagc gtcgtccacc
     1081 acttcggcgg ccggctcgcc gacaccctgg tcggcctcga catcctcgat ccgctggagg
     1141 cctaccggct gctcgccaag caggtcgccg ccaagctgat ggaggcgttc tcgtggcaga
     1201 agggccgcta cacctggacc ccgcgccacc ccaacccgtg gaagacgcgg tcgttgcacc
     1261 tcgatgcgtt ccgcgtgatc ggcgcgggcg cggcgcagct cggcgagccg ctgatcgagg
     1321 actggctgtc ggccaacctc aagggcttcg tggtccgcga gccgatccag gacgacgaga
     1381 tggcgcagtt cggcctcggc gatgcgctgc tccgggtcta cagcctgctc gacggccgga
     1441 cccggctcgg cgagctcgcc cacaaggtgc gctcgcccga ggcccggatg aacctgctgc
     1501 gcctgaccta cctgctcgtg cagactgacc tcgcccggct gtcgtgactg cgatcctcac
     1561 catccagggg ctggagaagc ggttcggcac ccgccgcgtg ctcgccggcg cgtcgttcgc
     1621 ggtccacgag cgcgaccgga tcggcatgat cggcgccaac ggcgcgggca agtcgacgct
     1681 gctcgagacc gtcgtgcacc gcgcgatggg cggcgacgac ccgacgctcg agcccgacgc
     1741 cggcctggtg acctggaagc gcgacctcgc gctcgagtac gtcgcccagg agccggtgct
     1801 cgacctcgag ctcgcggtcg gcgccgcgct ggcgcgcgac ggcgtgcccg atcacgagat
     1861 cgcgaccatc gccgccgcgc tcgagctgcc gccggcggac cagcggatcg cgacgctgtc
     1921 gctcggcgag cgccgccggg tcgcgctggc ccgcgcgctg ctcggcaagg ccgacgtgct
     1981 cgcgctcgac gagccgacca accacctcga cgccaccacc gtcgagtggc tcgagacccg
     2041 gctcgcggcc tggccgggcg cgctgatcgt cgtgacccac gaccgctact tcctcgaccg
     2101 ggtcgcgacc cgcatcctcg aggtcgaccg cggcaaggtc tacagctacg acggcgacta
     2161 cgccgagttc atcctgcagc aggcggcgcg gctggcggcc gaggccgagg tcgagtacca
     2221 gcgcgcgtcg ttcatccgcc gcgagatcga ctggatccgc cgcgggcc
//
LOCUS       DASVOR010001847         4735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_136246,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001847 DASVOR010000000
VERSION     DASVOR010001847.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4735
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136246"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VF516_34330"
     CDS             complement(<1..567)
                     /locus_tag="VF516_34330"
                     /inference="COORDINATES: protein motif:HMM:NF014316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEX8112876.1"
                     /translation="MEPRAPARAGAARRARARRRAGRRPGLTAVTRDELERARLSAAF
                     FADDATAVARKLIGCALVHGDRAGLVVETEAYLGPEDLASHARFGPTQRTSVMFGPGG
                     VSYVYLCYGIHQMFNIVTGGAGEGQAVLIRAIAPYLGLPDDPGTGRGPGKVTQALGLD
                     RRHDRRDLARGDLFVAAAVTPPRIARGPR"
     gene            complement(486..1382)
                     /locus_tag="VF516_34335"
     CDS             complement(486..1382)
                     /locus_tag="VF516_34335"
                     /inference="COORDINATES: protein motif:HMM:NF021613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HEX8112877.1"
                     /translation="MARTPSAEYLILDIETVPDTDRWQRPDLANSGEPAFPPTWAHRI
                     IVIGCLWLGHDYRLKRFGVIGGEPVTGAAPGDAPGDRPGDTPGDTRGAPSGPSAGAAG
                     AAADHRERALLEDFSRFVGRARPVLVTYNGRSFDLPVIVMRSLCHAIALPWYYRDRDV
                     RYRYSAEGHLDLCDWLAEHGAARAGKLDAIARLIGLPGKLGVDGSQVEGLYRAGQLAS
                     IQSYCLADVAQTALLFLRFRLLQGQIAPATYRERTTALLDTLAADGRVTEVFGQWNRE
                     RLLGPAQLAEPAPDDAPGGAPA"
     gene            1505..3196
                     /locus_tag="VF516_34340"
     CDS             1505..3196
                     /locus_tag="VF516_34340"
                     /inference="COORDINATES: protein motif:HMM:NF041770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX8112878.1"
                     /translation="MAVSRSGLALLALLPWFPVSDARADQQPGSCRVIDVDFTPGGFP
                     ATAKDPEIVPQIVAWLEKPGGEYVATLYITDQTGRYGLGNRPGRFDFNSGPNWPYGRR
                     VTVFPVWANRHGLKFQQVNYQNGEDFNLSHPAGDSSPEVHYCRPLMQGEPPWDAASCP
                     SMVFTDKGVFTSGTASALYPPRADLTASPKASGGQDSPSVEDYRNKNPFDAVSQASPR
                     LGMETQISWPIPPTLTNGDYLLYVEVALERDFNASYGQDRYPSPGATEIPWSEYGVAY
                     RGQPSVIYKVPFTISDTEAIAMTDTYAGYGDPGPDAVNGKTPSYFSPRPDPNPPDDTI
                     TTDRPGSGAARLLLTSKGGQTFRVRVDARTEPDLVAPGPPGAMAVVDTQSGGATLSFT
                     APGDDGMVGTVTGYDVRYRVGTTPISDADFNSANEARFTGKLVRAGDLQTLQIANLLP
                     ETPYSFAVRAFDDCHNSGTITTVMFTTAPRKVGEVDACFIATAAYGSLLANDVEMLRR
                     FRDRMLKHSVLGELAVETYYTFGPTVAGLIGESDLLRSTARDALAPLVRWVREVH"
     gene            complement(3203..3778)
                     /locus_tag="VF516_34345"
     CDS             complement(3203..3778)
                     /locus_tag="VF516_34345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112879.1"
                     /translation="MSLSRFPSSTSLASILALGLGLGAGCGGGDSCGPAGASDTGVIA
                     SGGGLTLTYGQFTGMQNNDCPASGAPKGLVSLTITGMQTDAGGAGFANLCVGRPDLLA
                     SQPLGLGFDAAAAVHVVDLTATTGGCTVKIDRSQPPSGTASASGLCDNGGNAAGFALV
                     LDATLTLTRTCGSTVDAVPITLRGRVAVAGP"
     gene            complement(3747..>4735)
                     /locus_tag="VF516_34350"
     CDS             complement(3747..>4735)
                     /locus_tag="VF516_34350"
                     /inference="COORDINATES: protein motif:HMM:NF023516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HEX8112880.1"
                     /translation="MDAIFDAGTHGIVTGHFLVDAITSASASSGAVNAAPFNEVRYEG
                     GIGYTHELGALTVGGDAKYSTESDYHSIYAGVHAQLELNQKNTVLGLGGGVSADTVSA
                     AAAQGPAMPTLRCDADHPADPDCPLDVYLVFGSVSQIVGKDTVVAATVDLSTLRGYQS
                     NPYRTAIVGEVLVPERHPTERNREAFAASIRHYLAATGTTFIGAYRYYLDDWHVHAHT
                     PELRIVQEVGRDIDAAIRFRYYTQTAAYFFRERYPADNAAMSQFVTDDTKLSAFTGYT
                     LEAKLGVLGEAFQLAGRWASARFEGMLNYTAQHNRFGNAVIAHVAVTIPFEY"
BASE COUNT          691 a         1640 c         1740 g          664 t
ORIGIN      
        1 ccgcggcccg cgggcgatcc gcggcggggt gacggcggcg gcgacgaaca ggtcgccgcg
       61 cgcgaggtcg cggcgatcgt gccggcgatc gagcccgagc gcctgggtca ccttgccggg
      121 gccgcgtccg gtcccgggat cgtcgggcag cccgaggtac ggggcgatcg cgcggatcag
      181 caccgcctgg ccctcgccgg cgccgccggt cacgatgttg aacatctggt ggatgccgta
      241 gcacaggtag acgtacgaca ccccgccggg tccgaacatc accgaggtgc gctgcgtcgg
      301 accgaaccgc gcgtggctcg cgaggtcctc ggggccgagg taggcctcgg tctcgaccac
      361 caggccggcg cggtcgccgt ggaccagcgc gcagccgatc agcttgcgcg cgaccgccgt
      421 ggcatcgtcg gcgaagaacg ccgcggacag ccgggcgcgc tcgagctcgt cgcgggtgac
      481 cgcggtcagg ccggggcgcc tcccggcgcg tcgtcgggcg cgggctcggc gagctgcgcc
      541 ggcccgagca ggcgctcgcg gttccactga ccgaagacct cggtgacccg gccgtcggcc
      601 gcgagcgtgt cgagcagcgc ggtggtgcgc tcgcgatagg tcgcgggtgc gatctggccc
      661 tgcaacaagc gaaatcgcag aaacagtagc gcggtctggg cgacgtcggc gaggcagtag
      721 ctctggatcg acgccagctg gccggcgcgg tacagcccct cgacctgcga gccatcgacg
      781 ccgagcttgc cgggcaggcc gatcagccgg gcgatggcat cgagcttgcc ggcgcgcgcg
      841 gcgccgtgct cggcgagcca gtcgcacaga tcgaggtggc cctcggcgct gtagcggtag
      901 cggacgtcgc ggtcgcggta gtaccagggc agcgcgatcg cgtgacacag cgaccgcatc
      961 acgatcaccg ggagatcgaa gctccggccg ttgtaggtca ccagcaccgg gcgcgcgcgg
     1021 ccgacgaacc gcgagaagtc ctcgagcagg gcgcgctcgc ggtgatcggc cgctgcgccg
     1081 gcggctccgg cgctcggtcc ggacggcgca cctctcgtat cccccggcgt atccccgggc
     1141 ctatcccccg gcgcatcccc cggcgcggcg ccggtgaccg gctcgccgcc gatcacgccg
     1201 aatcgcttga gccggtaatc gtgaccgagc cacaggcagc cgatcacgat gatccggtgc
     1261 gcccaggtcg gcgggaatgc cggctcgccg ctgtttgcca gatcgggccg ctgccagcga
     1321 tcggtgtcgg ggacggtctc gatatcgagg atcaggtact cggcgctcgg cgtgcgggcc
     1381 acacagcgac gctaggccga cggtgtgaca tcggacgcat gcgtgcgagc cgagccgcgg
     1441 ggacggcgcg gcgatcgtgc gcgatcgcac tttgttccgg tgctttgccc agcaataata
     1501 cggcatggcc gtgtcccgca gcgggttggc gctgctcgcg ctgttgccct ggtttcccgt
     1561 gtccgatgcg cgcgccgatc agcagcccgg atcgtgccgc gtgatcgacg tcgacttcac
     1621 gccgggcggc tttccggcga ccgccaagga cccggagatc gtgccgcaga tcgtcgcctg
     1681 gctggagaag ccgggcggcg agtatgtcgc gacgctgtac atcaccgacc agaccggccg
     1741 ctacggcctc ggcaaccggc cggggcggtt cgacttcaac agcggcccga actggccgta
     1801 cggccggcgg gtcacggtgt tcccggtgtg ggcgaaccgg cacggcctca agttccagca
     1861 ggtcaactac cagaacggcg aggacttcaa cctgagccac ccggccggcg acagctcgcc
     1921 cgaggtccac tactgccggc cgctgatgca gggcgagccg ccctgggacg ccgcgagctg
     1981 cccgtcgatg gtgttcaccg acaagggggt gttcacgtcg gggaccgcca gcgcgctcta
     2041 cccgccgcgc gccgacctga ccgcctcgcc caaggccagc ggtgggcagg actcgccgtc
     2101 ggtcgaggac tacaggaaca agaacccgtt cgatgcggtg tcgcaggcct cgccgcggct
     2161 cggcatggag acccagatca gctggccgat cccgccgacg ctgaccaacg gcgactacct
     2221 cctgtacgtc gaggtcgcgc tggagcgcga cttcaacgcc agctacggtc aggatcgcta
     2281 tccgtcgccg ggcgcgaccg agatcccgtg gtcggagtac ggcgtcgcgt accgcgggca
     2341 gccgtcggtg atctacaagg tgccgttcac gatcagcgac accgaagcga tcgcgatgac
     2401 cgacacctac gccggctacg gggaccccgg ccccgacgcg gtcaacggca agacgccatc
     2461 gtacttctcg ccccgcccgg accccaatcc acccgacgac acgatcacga ccgatcgccc
     2521 cggcagcggc gcagcgcgcc tgctgctgac cagcaagggc ggccagacct tccgggtccg
     2581 ggtcgacgcc cggaccgagc ccgacctggt cgcgccgggc ccgccgggcg cgatggccgt
     2641 ggtcgacacc cagtcgggcg gggcgacgct gtcgttcacc gcgcccgggg acgacggcat
     2701 ggtcggcacg gtcaccggct acgacgtgcg ctatcgggtc ggcaccacgc cgatcagcga
     2761 cgccgacttc aactcggcca acgaggccag gttcaccggc aagctcgtcc gcgccggcga
     2821 cctgcagacc ctgcagatcg cgaacctctt gcccgagacg ccgtacagct tcgcggtgcg
     2881 ggcattcgac gactgccaca acagcggcac gatcacgacc gtgatgttca cgaccgcgcc
     2941 gcgcaaggtc ggcgaggtcg atgcctgctt catcgcgacc gcggcctacg gctcgctgct
     3001 ggccaacgac gtcgagatgc tgcgccggtt ccgcgatcgc atgctcaagc acagcgtgct
     3061 cggcgagctc gcggtcgaga cctactacac gttcgggccg accgtggccg gcctgatcgg
     3121 cgagtccgat ctgctgcggt cgacggcgcg cgacgcgctc gccccgctgg tgcgctgggt
     3181 gcgcgaggtc cactgaggag ggctacggtc cggcgaccgc gacccggccg cgcagggtga
     3241 tcgggacggc gtcgacggtc gagccgcagg tccgggtcag cgtgagcgtg gcgtcgagca
     3301 cgagcgcgaa cccggcggcg ttgccgccgt tgtcgcacag cccgctggcg ctcgccgtgc
     3361 cgctcggggg ctggctgcgg tcgatcttca ccgtgcagcc gccggtggtg gcggtcaggt
     3421 cgaccacatg gacggctgcc gcggcatcga agccgagccc gaggggctgg cttgcgagca
     3481 gatccgggcg gccgacgcac aggttggcaa acccggcacc gccggcgtcg gtctgcatgc
     3541 cggtgatcgt caagctgacc agacccttgg gcgccccgct ggcggggcag tcgttgttct
     3601 gcatgccggt gaactggccg taggtcaagg tcagcccgcc gccgctcgcg atcacgccgg
     3661 tgtcggacgc gccggcgggg ccgcagctgt ccccaccgcc gcagcccgcg ccgaggccga
     3721 ggccgagcgc aaggatggat gcgaggctag tactcgaagg gaatcgtgac agcgacatgg
     3781 gcgatcaccg cgttgccgaa ccggttgtgc tgggcggtgt agttcagcat gccttcgaac
     3841 cgcgcgctcg cccaccggcc ggcgagctgg aacgcctcgc cgagcacacc gagcttggcc
     3901 tccagggtat agccggtgaa ggccgatagc ttggtgtcgt cggtgacgaa ctggctcatc
     3961 gcggcgttgt ccgccgggta gcgttcgcgg aagaagtacg cggcggtctg ggtgtagtag
     4021 cggaaccgga tcgcggcgtc gatgtcgcgc ccgacctcct ggacgatccg gagctcgggc
     4081 gtgtgggcgt ggacgtgcca gtcgtcgagg tagtagcggt atgcgccgat gaacgtcgtg
     4141 ccggtcgcgg cgaggtagtg gcggatcgac gcggcgaacg cctcgcggtt gcgctcggtg
     4201 gggtggcgct cggggaccag gacctcgccg acgatcgcgg tgcggtacgg gttggactgg
     4261 tagccgcgca gggtcgacag atcgaccgtg gccgcgacga ccgtgtcctt gccgacgatc
     4321 tgggacaccg atccgaacac caggtagacg tcgagcgggc agtccggatc ggccgggtgg
     4381 tccgcgtcgc accgcagggt cggcatcgcc gggccctgcg cggcggcggc gctgaccgtg
     4441 tcggcactga ccccgccgcc gagcccgagc acggtgttct tctggttgag ctcgagctgg
     4501 gcgtgcacgc cggcgtagat cgagtggtag tcggactcgg tcgagtactt ggcgtcgccg
     4561 ccgacggtca gcgcgccgag ctcgtgggtg tagccgatgc cgccctcgta gcgcacctcg
     4621 ttgaacggcg ccgcgttgac cgcgccggac gacgccgacg ccgaggtgat cgcgtcgacc
     4681 aggaagtggc cggtgacgat gccgtgggtg ccggcgtcga agatcgcgtc catca
//
LOCUS       DASVOR010001848         2866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_427818,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001848 DASVOR010000000
VERSION     DASVOR010001848.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2866
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427818"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="VF516_34355"
     CDS             complement(<1..236)
                     /locus_tag="VF516_34355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112881.1"
                     /translation="MLAERTDPLIGTMLGKSYRVLERIGSGGMAVVYLVEHQTLLKRF
                     AVKVLSSDLASSLEARARFTQEAHAASQLDHENIV"
     gene            248..676
                     /locus_tag="VF516_34360"
     CDS             248..676
                     /locus_tag="VF516_34360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112882.1"
                     /translation="MAGIDGAGIRTGIRNTEHIEEIVTINETTSGESELQSRMAMDSL
                     ARDIYVRTRLCPMRGRIVTTARAHQVHVIAARAALTHDETRRHGKTCSTCSNGVLPGA
                     HCANHRVRRCGRLRDAPFSRVARYQIYQIYDVNQFYAGRA"
     gene            complement(648..2213)
                     /gene="der"
                     /locus_tag="VF516_34365"
     CDS             complement(648..2213)
                     /gene="der"
                     /locus_tag="VF516_34365"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEX8112883.1"
                     /translation="MTAPLIAIVGRPNVGKSTLFNRLVGGRPALVHGTPGLTRDRRYG
                     ECDYFGAEMRVVDTGGLDPDAARDVIGAGIHRQAMRAIGEADALVLVVDGRAGLSAVD
                     QDLAGQLRATGKPVFLAVNKIDHPTRDDLVHEFHALGLGPPYPVSAAHGRGVDPLLEA
                     IAAAVGARRPEPTEPEGEPDPWDILTSEPDQPEESEDSEESEPFEGSEDAEGSEDADR
                     DRAGRRGDPIEPRDATRGGVNGRALTGQRGRESPLSIDLRVAFVGRPNAGKSSLTNRL
                     LGEERSLVHHVAGTTTDPVDSAFSIGGRDYVLVDTAGIRRKAKIDADIEKLAVTMALS
                     QIERCDVAVLVIDAELGPAEQDARIAGMVEQAGRGLVIALNKSDRVVGEAARARLREL
                     TQDTFHFLSWAPTVMLSAQRGDGVDRLLDAVDKVAREHARRVPTAQLNRFFAEVIEEM
                     PPPLHHGRAVRIHYLTQATVRPPTFVLWANTDGLAPSYKRFLTNRLRQRYGFAGTPIR
                     IAVKVRPDRHKTD"
     gene            complement(2210..>2866)
                     /gene="era"
                     /locus_tag="VF516_34370"
     CDS             complement(2210..>2866)
                     /gene="era"
                     /locus_tag="VF516_34370"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEX8112884.1"
                     /translation="LLLVDATDRRGRLPERLAEADAAALGDASRQHPIVIGLNKVDRV
                     AKLELLPVIEAWASFAPGVEVVPICALTGDGVAALERAIALRLPLGPALFPPDMVTDR
                     SPQFIAQEIIREQLYHQLGKELPYASAVQIESWTEKSPSELAIGAVIVVERESQKAIV
                     VGRGGARVRELGIAARHAVGEALGKTVHLSLFVKVISEWSRGEAGLRRLGYAGEDGAP
                     "
BASE COUNT          422 a         1012 c         1001 g          431 t
ORIGIN      
        1 acgatgttct cgtgatcgag ctgcgacgcc gcgtgggcct cctgggtgaa ccgcgcgcgg
       61 gcctccaggc tcgacgccag gtcgctcgac agcaccttga ccgcgaaccg cttgagcagc
      121 gtctggtgct cgaccaggta cacgacggcc atgccgccgg agccgatccg ctcgagcact
      181 cgatagctct tgccgagcat cgtgccgatc agcgggtcgg ttcgctcagc cagcacgcgc
      241 agacctcgtg gccgggatcg acggagccgg gatcagaacc gggatcagaa acacagaaca
      301 catcgaagaa atagtcacca tcaacgaaac aaccagtggt gaatccgagc tgcagtctcg
      361 catggccatg gactcgctcg cacgtgacat ttacgtacgg acacgacttt gcccgatgcg
      421 cggccgcatc gtgaccacgg cgcgcgcgca ccaggtccac gtgatcgcgg cgcgcgctgc
      481 gctgacgcac gatgagacca ggcgccacgg caaaacctgt tcaacctgtt caaacggtgt
      541 gttgcccggt gcgcactgcg caaatcacag ggtgcgccgg tgtgggcgat tgcgcgatgc
      601 gccgttttct cgcgttgccc gataccagat ctaccagatc tatgacgtca atcagtttta
      661 tgccggtcgg gcctaacttt gacggcgatt cggatcggcg tgcctgcgaa tccgtagcgc
      721 tggcgcagcc ggttcgtgag gaagcgcttg tacgacggcg cgaggccatc ggtgttggcc
      781 cacagcacga acgtcggcgg cctcacggtg gcctgggtca ggtagtggat gcggaccgcg
      841 cggccgtggt gtagcggcgg cggcatctcc tcgatgacct cggcgaagaa ccggttgagc
      901 tgcgcggtcg ggacgcggcg cgcgtgctcg cgcgcgacct tgtcgacggc gtcgagcagg
      961 cgatcgacgc catcgccgcg ctgcgccgac agcatcacgg tcggcgccca cgacaggaag
     1021 tggaacgtgt cctgggtcag ctcgcgaagc cgggcgcggg cggcctcgcc gaccacccgg
     1081 tcggacttgt tgagcgcgat gaccaggccg cggccggcct gttcgaccat gccggcgatc
     1141 cgggcgtcct gctcggccgg tccgagctcg gcgtcgatga ccagcacggc gacatcgcag
     1201 cgctcgatct gcgacagcgc catcgtgacc gcgagcttct cgatgtcggc gtcgatcttt
     1261 gccttgcggc ggatcccggc ggtgtcgacc agcacgtagt cgcggccgcc gatcgagaac
     1321 gccgagtcga ccggatcggt ggtcgtgccg gcgacgtggt ggaccagcga gcgctcctcg
     1381 ccgagcagcc ggttggtcag cgacgacttg ccggcgttgg gccggccgac gaacgcgacc
     1441 cgcaggtcga tcgagagggg actttcccgt cccctctggc cggtcaaagc ccggccattc
     1501 acccctccgc gcgtcgcgtc gcgcggctcg atcggatcgc cgcggcggcc cgcccggtcg
     1561 cggtcggcat cctcggatcc ttcagcgtct tcggatcctt cgaacggttc ggattcctcg
     1621 gaatcttcgg attcctcggg ctggtcgggc tccgacgtga ggatgtccca cggatcgggc
     1681 tccccttccg gctcggtcgg ctcggggcgc cgggcaccga ccgccgcggc gatcgcctcg
     1741 agcagcggat cgacgccgcg gccgtgcgcc gccgacaccg gatacggcgg gccgaggccg
     1801 agcgcgtgga actcgtggac cagatcgtcg cgcgtggggt gatcgatctt gttgaccgcg
     1861 aggaacaccg gcttgccggt cgcgcgcagc tggccggcga gatcctggtc gaccgcggac
     1921 agcccggcgc ggccgtcgac cacgagcacc agtgcgtcgg cctcgccgat cgcgcgcatc
     1981 gcctggcggt ggatcccggc gccgatcacg tcgcgcgcgg cgtcgggatc cagcccgccg
     2041 gtgtcgacca cgcgcatctc ggccccgaag tagtcgcact cgccgtagcg gcgatcgcgg
     2101 gtcagcccgg gcgtgccgtg gaccagcgcc gggcggccgc cgaccaggcg gttgaacagc
     2161 gtcgacttgc cgacgttggg ccggccgacg atcgcgatca gcggcgcggt cacggcgcac
     2221 cgtcctcgcc ggcgtagccc agccggcgca gcccggcctc gccgcggctc cactcgctga
     2281 tcaccttgac gaacagcgac aggtgcacgg tcttgccgag cgcctcgccg accgcatggc
     2341 gcgccgcgat cccgagctcg cggacgcgcg ccccgccccg cccgaccacg atcgccttct
     2401 gcgactcgcg ctcgaccacg atcaccgcgc cgatcgcgag ctcggacggc gacttttcgg
     2461 tccacgactc gatctgcacc gcgctggcgt acggcagctc cttgccgagc tggtggtaca
     2521 gctgctcgcg gatgatctcc tgggcgatga actgcggcga gcgatcggtc accatgtcgg
     2581 gcgggaacag cgcgggcccg agcggcaggc ggagcgcgat cgcgcgctcg agcgccgcga
     2641 ccccgtcgcc ggtcagcgcg cagatcggca cgacctcgac gcccggggcg aagctcgccc
     2701 acgcctcgat caccggcagc agctcgagct tggcgacgcg gtcgaccttg ttgagcccga
     2761 tcacgatcgg gtgctgccgg ctggcgtcgc cgagcgcggc ggcgtcggcc tcggcgagcc
     2821 gctcgggcag ccggccgcgc cgatcggtgg cgtcgaccag cagcag
//
LOCUS       DASVOR010001849         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_545647,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001849 DASVOR010000000
VERSION     DASVOR010001849.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545647"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VF516_34375"
     CDS             <1..469
                     /locus_tag="VF516_34375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112885.1"
                     /translation="APPVMPRPRPRPSLFRGGGGGGGLAIAASTSSAMVCPTCRTEYH
                     GLTYCTRDARRLVPPEELLGGPGLGPMRALSRSAGLVCMACRRAYEPGLRSCPHDGNE
                     LVPFPVYNATRPRRARTTEPAGVIARICPVCGTKYDLNARFCGHDAGQLVVIN"
     gene            complement(480..>1638)
                     /locus_tag="VF516_34380"
     CDS             complement(480..>1638)
                     /locus_tag="VF516_34380"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX8112886.1"
                     /translation="VVRPSPPPASFTPPSGAATGDAAEVRRRLQGTLLTCSVYKRSPP
                     DHGVDPIAVLFTADSLGDPKGVLRTDKNTIAAVDHAISALGMTDATKNLVAVPLFHAY
                     GWDLGFLPTLKLGSTMFLEEEISARRIGKLVREHEVDVLPGTPSMYAELARLPTAKKL
                     EIKSPRYLAAGSRLDQTVADEFFDKYGIRLLSCYHSTEAATVTLEDTGKYPTTVGKPI
                     DGVEIKITSPDGSQLKSTKPSDGLIWVKSKTLSPKSIGPFDDERAPASARASGMVAIG
                     AIDKTGWLRTGDLGRLDKNGRLQLTGREDDVVKVDGKRVALGEVEGCLEAFPKIKAAQ
                     ATVVTDPMAGAMVVARVVMKQKCGAEEIIDHCARNLAPYKVPRRIEFCEAI"
BASE COUNT          211 a          600 c          564 g          263 t
ORIGIN      
        1 cgctcccccg gtgatgccgc gcccccgtcc acgcccatcc ctgttccgcg gcggcggcgg
       61 tggtggcggg ctcgcgatcg cggcctccac ctcgagcgcg atggtctgcc cgacctgccg
      121 caccgagtac cacggcctga cctactgcac gcgcgacgcg cgccggctgg ttccgcccga
      181 ggagctgctc ggcggaccgg gcctgggacc gatgcgcgcg ctgtcccgca gcgcgggcct
      241 ggtgtgcatg gcgtgccgcc gggcctacga gcccggtctg cgcagctgcc cgcatgacgg
      301 caacgagctg gtgccgtttc cggtctacaa cgccacgcgt ccgcggcgcg cccgcaccac
      361 cgagcccgcc ggcgtgatcg cccggatctg cccggtgtgc ggcaccaagt acgatctcaa
      421 cgcgcgattc tgcggccacg acgccggcca gctggtcgtc attaactgat cgggccggtt
      481 cagatcgctt cgcagaactc gatccgcctg gggaccttgt agggcgccag gttgcgcgcg
      541 cagtggtcga tgatctcctc ggcgccgcac ttctgcttca tcacgacgcg cgcgaccacc
      601 atcgcgcccg ccatcggatc ggtcaccacg gtggcctggg ccgccttgat cttggggaac
      661 gcctcgaggc agccctcgac ctcgccgagc gcgacccgct tgccgtcgac cttgaccacg
      721 tcgtcctcgc ggccggtcag ctgcaggcgg ccgttcttgt cgagccggcc gaggtcgccg
      781 gtccgcagcc acccggtctt gtcgatcgcg ccgatcgcga ccatgccgga cgcccgggcc
      841 gaggccggcg cgcgctcgtc gtcgaacggg ccgatcgact tcggcgacag cgtcttgctc
      901 ttgacccaga tcaggccgtc gctcggcttg gtggacttga gctgcgagcc gtcgggcgag
      961 gtgatcttga tctcgacgcc gtcgatcggc ttgccgaccg tggtcgggta cttgccggtg
     1021 tcctccagcg tgaccgtcgc ggcctcggtg gagtggtagc agctgagcag ccggatcccg
     1081 tacttgtcga agaactcgtc ggcgaccgtc tggtcgagcc gcgatccggc ggcgaggtag
     1141 cgcggcgact tgatctcgag cttcttcgcg gtcggcagcc tggcgagctc ggcgtacatc
     1201 gacggcgtgc cgggcagcac gtcgacctcg tgctcgcgca ccagcttgcc gatccggcgc
     1261 gccgagatct cctcctcgag gaacatcgtc gagccgagct tcagcgtcgg caggaacccg
     1321 aggtcccagc cgtaggcgtg gaacagcggc accgcgacca ggttcttggt cgcgtcggtc
     1381 atgccgagcg cgctgatcgc atgatcgacc gccgcgatcg tgttcttgtc ggtgcgcagc
     1441 acgcccttgg gatcgccgag cgagtccgcc gtgaacagca ccgcgatcgg atcgacgccg
     1501 tgatccggcg gcgagcgctt gtacacgctg caggtcagca gcgtgccctg cagccggcgc
     1561 cggacctccg cggcatcgcc agtcgccgcg cccgacggcg gcgtgaacga ggccggcggc
     1621 ggcgacggcc gcacgacc
//
LOCUS       DASVOR010001850         2680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340430,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001850 DASVOR010000000
VERSION     DASVOR010001850.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2680
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340430"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1349
                     /locus_tag="VF516_34385"
     CDS             93..1349
                     /locus_tag="VF516_34385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEX8112887.1"
                     /translation="MANHKVADLKLADAGRLRIDWAETRMPVLMALREKGKQDKPLRG
                     MKVAGCLHVTKETAVLIRTIIAAGAEVSWSGCNPLSTNDEVAAALAADGVSIYAWHGL
                     STQEFYWCIERTLDFGPTLTLDDGADLIFTVHNKHQHLVKGIIGGTEETTTGVHRLRA
                     MADDGKLLYPVFAVNDSETKWDFDNVYGTGQSTLDGVLRATSILLAGKTVVTGGYGHC
                     GRGIAERARGLGASVIATEIKPTQALKAILEGHRVMPMDQAARLGDVFITATGMKDVL
                     VGRHFDVLKDGAVICNTGHYDCEINIGDLEARAKGRRTVRPNCEEFTMKDGRRIYLLA
                     QGRLVNLAAAEGHPSEVMDMSFSNQYLALCKLAKEGKALDKKVYQLPPELDQEIAGVK
                     LQTMGVSIDKLTSEQVAYASDYSAGT"
     gene            1608..2237
                     /locus_tag="VF516_34390"
     CDS             1608..2237
                     /locus_tag="VF516_34390"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEX8112888.1"
                     /translation="MARTSLYIDGFNLYYRALKGTPFKWLDLKKLAQQILATHNRITC
                     IKYFTARVSGHQNPSSPRDQEAYLAALSTIPEVQIIYGHFLAKTIRRPLVNPVPGLPR
                     AVEVHTSEEKGSDVNLAVHLLHDAWSARFDVAVVVSNDSDLEAPIRIVKDELRKPVGL
                     LCPHDGKPSPQLKAAATFVRHIRDTHLRASQFPSPIVHNGRNIVKPSDW"
     gene            complement(2259..>2680)
                     /locus_tag="VF516_34395"
     CDS             complement(2259..>2680)
                     /locus_tag="VF516_34395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112889.1"
                     /translation="ACRYGLDCIDGRCAAPPVLGQPCTSACRDLGTTCSSVTHTCVAV
                     GLVGAPCSIGASDCAPEYVCDRTGHCSAGIALGKPCGAGDQCADDRAACDTPPDQLTG
                     VCALPKPDGSPCARDAVCDSLHCDPFTTRCAPEPVCL"
BASE COUNT          469 a          943 c          873 g          395 t
ORIGIN      
        1 atgatctggc aggcgcgccg ccgacggcgc ggccctcgcg cttgatttac cgacgagccc
       61 gggctatgag gccgcaacca aaggagtgtc tcgtggcgaa tcacaaagtg gccgatctca
      121 agcttgccga cgcgggccgc ctgcggatcg actgggctga aactcgcatg ccggtgctga
      181 tggcgctgcg cgaaaagggc aagcaggaca agccgctgcg cgggatgaag gtcgccggct
      241 gcctgcacgt caccaaggag accgcggtcc tgatccggac gatcatcgcg gccggcgccg
      301 aggtcagctg gtcgggctgc aacccgctgt cgaccaacga cgaggtcgct gcggcgctcg
      361 ccgccgatgg cgtctcgatc tacgcctggc acggcctgtc gacccaggaa ttctactggt
      421 gcatcgagcg cacgctcgac ttcgggccga cgctcaccct ggacgatggc gccgacctga
      481 tcttcacggt gcacaacaag caccagcacc tggtcaaggg catcatcggc ggcaccgagg
      541 agaccacgac cggcgtccac cgcctgcgcg cgatggccga cgacggcaag ctgctgtacc
      601 cggtgttcgc ggtcaacgac agcgagacca agtgggactt cgacaacgtc tacggcaccg
      661 gccagtcaac gctcgacggc gtgctgcgcg cgacgtcgat cctgctggcc ggcaagaccg
      721 tggtcaccgg cggctacggc cactgcggcc gcggcatcgc ggagcgcgcc cggggcctgg
      781 gcgccagcgt gatcgccacc gagatcaagc cgacccaggc gctcaaggcg atcctcgagg
      841 gccaccgcgt gatgccgatg gaccaggccg cccggctcgg cgacgtgttc atcaccgcga
      901 ccggcatgaa ggacgtgctg gtcggccgcc acttcgacgt cctgaaggac ggcgcggtga
      961 tctgcaacac cggccactac gactgcgaga tcaacatcgg cgacctcgag gcccgcgcca
     1021 agggccgccg caccgtgcgc cccaactgcg aggagttcac gatgaaggac ggccggcgga
     1081 tctacctgct cgcccagggc cgcctggtca acctcgccgc cgccgagggc caccccagcg
     1141 aggtcatgga catgtcgttc tccaaccagt acctcgcgct gtgcaagctg gccaaggagg
     1201 gcaaggcgct cgacaagaag gtctaccagc tgccccccga gctcgatcag gagatcgccg
     1261 gcgtcaagct gcagaccatg ggcgtctcga tcgacaagct cacctccgag caggtcgcgt
     1321 acgccagcga ctactcggcc ggcacctgac caggtcaccc ggatcggcgt tgctacgccg
     1381 gacgtgctgc tgcagcggcc cagcgccgta atttcgcggg ccgcaaaatc aaatagttcc
     1441 atgatcttgc gatgatacat gtcaaaagct ggttgacgta ggcccgggat tgccgtatct
     1501 acgttgcata cccgctggca gcgtgctgct agctcaggag ccggtccttc gggatgcggc
     1561 tcttgctttt ttcggccgcc tggcgcgagc gtggcccagg tgctccggtg gcgaggacct
     1621 cgctctacat cgacggcttc aacctctact accgcgccct caagggcacg ccgttcaagt
     1681 ggctcgacct caagaagctg gcccagcaga tcctggccac ccacaaccgg atcacctgca
     1741 tcaagtactt caccgcgcgc gtcagcggac accagaaccc cagcagcccc cgagatcaag
     1801 aggcgtacct cgccgccctc tcgacgattc ccgaggtcca gatcatctac ggtcactttc
     1861 tcgcgaagac catccgccgc ccgctcgtga accccgtacc tggcctgccg cgtgcggtcg
     1921 aggtgcatac cagcgaggag aaaggctccg acgtcaacct ggccgtccat ctcctccatg
     1981 atgcatggtc cgccaggttc gacgtcgcgg tcgtggtctc gaacgacagt gacctcgagg
     2041 cgccgatccg gatcgtcaag gacgagcttc gcaagccggt cgggctcctg tgcccgcacg
     2101 acggcaaacc atcccctcag ctcaaggcgg cggcgacatt tgtgcgccat atccgggaca
     2161 ctcacctgcg ggcgagtcag ttcccgtcgc cgatcgtcca caacggccgg aacatcgtga
     2221 agccgtctga ctggtgacgg cctggcgagg tccgtatgtc agaggcagac cggctcgggc
     2281 gcgcagcggg tcgtgaacgg atcgcagtgc aggctgtcgc acacggcgtc gcgcgcgcac
     2341 ggcgagccgt cgggcttggg gagcgcgcac acgccggtga gctgatccgg cggcgtgtcg
     2401 caggcggcgc ggtcgtcggc gcactgatcg ccggcgccgc acggcttgcc gagcgcgatc
     2461 ccggcgctgc agtgcccggt ccgatcgcac acgtactcgg gcgcgcagtc cgacgcgccg
     2521 atgctgcacg gcgcgccgac cagcccgacc gcgacgcacg tgtgcgtcac cgagctgcac
     2581 gtcgtgccga gatcgcggca ggcggacgtg cacggctggc cgagcaccgg cggcgccgcg
     2641 caccggccgt cgatgcagtc gagcccgtac cggcacgccg
//
LOCUS       DASVOR010001851         4756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_427851,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001851 DASVOR010000000
VERSION     DASVOR010001851.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427851"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="VF516_34400"
     CDS             <1..757
                     /locus_tag="VF516_34400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEX8112890.1"
                     /translation="SKTVMTMTFMDGVKITKKDALAGLALDPYDVATKLIKVFYKQLF
                     VDRFFHADPHPGNFFVQRGPDGQARIVVLDLGSATDLRDNLADGMFDILSGLMTRKDD
                     LGVRGIDTMGFMAEGGDRALLERTVRRYFEKLLSINITDFGKIDPAVAQQLSDPHMKR
                     EDLRELMKSVAYPEGWFYVERAAVIMFGLSSTLAPRLNGIHVGFPYVTQFIMERNAKR
                     LAEQTARKAAEAAKPPAAEAAAKLSDESEALLN"
     gene            913..1470
                     /locus_tag="VF516_34405"
     CDS             913..1470
                     /locus_tag="VF516_34405"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEX8112891.1"
                     /translation="MFDIVLAADLDWGIGKDNALPWPKLRGDLQHFKRVTSTASEGRR
                     NAVVMGRKTWESKEVAGKPLPNRLNVVVSRQALAVPPGVVLAHSVDEALAVGGVESIF
                     VVGGAGLLRDAIERPDLRFVYLTRIEQRFDCDIRMPDLDARGFIRDAWEGEREAEEFE
                     VRYRIERLKPPGRRAVDAVTPRPGT"
     gene            complement(1448..2239)
                     /locus_tag="VF516_34410"
     CDS             complement(1448..2239)
                     /locus_tag="VF516_34410"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEX8112892.1"
                     /translation="MRPYLDLLRHVLDHGERRTDRTGTGTIAVFGAQTRYDLREGFPL
                     VTTKKVLFPAVVRELLWFLRGSTNIHDGLTEHTPIWDAWADASGELGPIYGSQWRHWG
                     ARNGGPGIDQVTGAIETIKRDPSSRRIIVSAWNVADLPDMRLPPCHALFQFHVHDGQL
                     DCQLYQRSADLALGVPFNIASYALLTAMIAQECRLSPRYFIHTLGDAHIYLNHVEGVK
                     LQLERRPFPLPRLVLADKPVLEQRFEDIALEGYQHHPFIKFPVAV"
     gene            2273..3139
                     /locus_tag="VF516_34415"
     CDS             2273..3139
                     /locus_tag="VF516_34415"
                     /inference="COORDINATES: protein motif:HMM:NF024740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEX8112893.1"
                     /translation="MAVPPPEPATIAELLDRYDGVLLDAYGVLVDARGLLPGAAALFA
                     ELDRRGTPFAIVTNDASRSPATYAARFARHGLVVAPERIVTSGGLLPAYFRDRGLAGA
                     PTCVLGTEDSFAYVRAGGGVPVALEPGMAVDAVAVCDDSGTPFVDGIELAFSAVVRAV
                     EAGRRPALVLPNPDLVYPKGGAEYGFTAGAMALLIEAALGRRFPGHGLVFDRLGKPGP
                     QLFAEARRRVGGRVVMIGDQLETDIAGACAAGIDAALLAGVSRWPPAPPASPTSMASG
                     VAPRYLLATIEP"
     gene            3136..3432
                     /locus_tag="VF516_34420"
     CDS             3136..3432
                     /locus_tag="VF516_34420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112894.1"
                     /translation="MIQRFCFVKLRDDEVAIRGEIAALLAAELREAGADAVVGLPADA
                     SAARWDLSIMITAASLAAWHALAQVPAVVRVFDRLAQRAPVVKAWTFAAVGDDH"
     gene            complement(3510..4364)
                     /locus_tag="VF516_34425"
     CDS             complement(3510..4364)
                     /locus_tag="VF516_34425"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEX8112895.1"
                     /translation="MGTRFEVAADEAGLRLDQVVPRHVAGLSRRQARAVIDLGGVFVD
                     RARVKVAGRPVRAGQVIEVHVGGALDRDRPPPEPGIVFVDDHVIVADKPAGLVTAPTP
                     ESDRGDLLDQLARRHGEVYLVHRLDLPTSGLLVFARTREANKRLGEAFARHDVDREYR
                     AVAVGDVAPQTLDRPIQGKRAVTHIGAIEHLAGATLVSARLETGRSHQIRIHLAGIGH
                     PVAGDHQHGGETARTFVPRAPRLALHAAVLGFTHPATGQRVRFERALPDDLAAWIDRL
                     RATRRAEG"
BASE COUNT          605 a         1728 c         1726 g          697 t
ORIGIN      
        1 gagcaagacg gtgatgacca tgacgttcat ggacggcgtc aagatcacca agaaggacgc
       61 gctcgccggc ctcgcgctcg acccgtacga cgtcgcgacc aagctgatca aggtgttcta
      121 caagcagctg ttcgtcgacc gcttcttcca cgccgatccg caccccggga acttcttcgt
      181 ccagcgcggc ccggatggcc aggcccggat cgtggtgctc gacctcggct cggcgaccga
      241 cctgcgcgac aacctcgccg acggcatgtt cgacatcctg tcgggcctga tgacgcgcaa
      301 ggacgacctc ggggtgcgcg gcatcgacac catggggttc atggccgagg gcggcgaccg
      361 cgcgctgctc gagcgcaccg tgcggcgcta cttcgagaag ctgctcagca tcaacatcac
      421 cgacttcggc aagatcgatc ccgcggtggc ccagcagctg tcggatcccc acatgaagcg
      481 cgaggacctg cgcgagctga tgaagtcggt ggcctacccc gagggctggt tctacgtcga
      541 gcgcgcggcg gtgatcatgt tcgggctgtc gtcgacgctg gcgccccggc tcaacggcat
      601 ccacgtcggc ttcccgtacg tcacccagtt catcatggag cgcaacgcca agcggctggc
      661 cgagcagacc gcgcgcaagg ccgccgaggc cgccaagccg ccggccgccg aggccgcggc
      721 caagctctcc gacgagtccg aggcgctgct gaactgaccg cagacgcgcg gccctgcacc
      781 tgacggctct gggctcgctt gcgacgcctg ctcctggatg cgatcccccg gctgacgcgt
      841 tctgccgcac cctgccggca ctgccttcgg ttccactccc gggcggcacg gctcgcgtgc
      901 tagcgtcgca gcgtgttcga catcgtcctc gcagcggacc tcgactgggg gatcggcaag
      961 gacaacgccc tgccgtggcc caagctgcgc ggcgacctcc agcacttcaa gcgcgtcacg
     1021 tcgacggcga gcgagggccg gcgcaacgcg gtcgtgatgg gccgcaagac ctgggagagc
     1081 aaggaggtcg cgggcaagcc gctgcccaac cggctcaacg tcgtggtgtc gcgccaggcg
     1141 ctcgcggtgc cgcccggcgt ggtgctcgcg cactcggtcg acgaggcgct cgcggtcggc
     1201 ggggtcgagt cgatcttcgt ggtcggcggc gccggcctgc tgcgcgacgc gatcgagcgc
     1261 cccgacctgc ggttcgtgta cctgacgcgg atcgagcagc ggttcgactg cgacatccgg
     1321 atgcccgacc tcgatgcgcg cggcttcatc cgcgacgcct gggagggcga gcgcgaggcc
     1381 gaggagttcg aggtccgcta ccggatcgag cgcctgaagc cgcccgggcg ccgggcggtc
     1441 gacgcggtca caccgcgacc gggaacttga tgaacgggtg gtgctggtag ccctcgagcg
     1501 cgatgtcctc gaaccgctgc tccagcaccg gcttgtcggc gagcaccagc ctcggcagcg
     1561 ggaacggccg gcgctcgagc tggagcttca cgccctcgac gtggttcagg tagatgtggg
     1621 cgtcgccgag cgtgtggatg aagtagcgcg gggacagccg gcactcctgg gcgatcatcg
     1681 cggtgagcag cgcgtagctc gcgatgttga acggcacgcc gagcgcgagg tcggccgagc
     1741 gctggtacag ctggcagtcg agctgcccgt cgtggacgtg gaactggaac agcgcgtggc
     1801 acggcggcag ccgcatgtcc ggcaggtcgg cgacgttcca cgcgctgacg atgatccggc
     1861 gcgacgacgg atcgcgcttg atcgtctcga tcgccccggt gacttggtcg atgcccgggc
     1921 cgccgttccg cgcgccccag tgccgccact gggaaccgta gatcggtccg agctcgccgc
     1981 tggcgtcggc ccacgcgtcc cagatcggcg tgtgctcggt cagtccatca tgaatgttgg
     2041 tcgagccgcg caggaaccac aggagctcgc ggaccaccgc cgggaacagc accttcttgg
     2101 tggtgaccag cgggaacccc tcgcgcaggt cgtaccgggt ctgagcgccg aacaccgcga
     2161 tcgtcccggt gccggtgcga tcggtgcggc gctcgccgtg atcgagcacg tggcgcagga
     2221 gatcgaggta cggccgcatg ccctgcgtat agcacctgcg tatgatcccc gcgtggccgt
     2281 acctcccccg gaacccgcga cgatcgccga gctgctcgac cgctacgacg gcgtcctgct
     2341 cgacgcctac ggcgtgctgg tcgacgcccg cggcctgctc cccggcgccg ccgcgctgtt
     2401 cgccgagctc gaccgccgcg gcacgccgtt cgcgatcgtc accaacgacg cgtcgcgctc
     2461 gccggcgacc tacgccgcgc ggttcgcgcg ccacgggctg gtggtcgcgc ccgagcggat
     2521 cgtgacctcg ggcggcctgt tgccggcgta cttccgcgac cgcgggctgg ccggcgcgcc
     2581 gacctgcgtg ctcggcaccg aggattcgtt cgcctacgtc cgcgcgggcg gcggcgttcc
     2641 ggtcgcgctg gagccgggca tggcggtcga cgcggtcgcg gtgtgcgacg actccggcac
     2701 gccgttcgtc gacggcatcg agctggcgtt ctcggcggtg gtgcgcgcgg tcgaggccgg
     2761 ccggcgcccg gcgctggtcc tgcccaaccc cgacctggtg tatcccaagg gcggcgccga
     2821 gtacggcttc accgcgggcg cgatggcgct gctgatcgag gccgcgctcg gccgccggtt
     2881 ccccggccac ggcctggtgt tcgaccggct cggcaagccg gggccgcagc tgttcgccga
     2941 ggcccggcgc cgggtcggcg gccgcgtggt gatgatcggc gatcagctgg agaccgacat
     3001 cgcgggcgcg tgcgccgccg ggatcgacgc cgcgctgctc gccggagtgt cgcgctggcc
     3061 gccggcgccc ccggcgtcgc cgacatcgat ggcgtccggc gttgcgccgc gctacctgct
     3121 cgctaccatc gaaccgtgat ccagcggttc tgcttcgtca agctgcgcga cgacgaggtc
     3181 gcgatccgcg gcgagatcgc cgcgctcctg gccgccgagc tgcgcgaggc cggcgccgac
     3241 gccgtggtcg gtctgcccgc cgatgccagc gcggcgcgct gggatctgtc gatcatgatc
     3301 accgccgcct cgctggcggc gtggcacgcc ctcgcccagg tcccggcggt ggtccgcgtg
     3361 ttcgaccggc tcgcgcagcg cgccccggtc gtcaaggcgt ggacgttcgc ggcggtgggc
     3421 gacgatcact gagccgcggg cgcgcggagc cgatcggttg atccacaggg ggccttcccg
     3481 ccccctcgtg cccggcaaag ccgggcaatt cacccctccg cgcgtcgcgt cgcgcggagc
     3541 cgatcgatcc atgccgccag gtcgtcgggc agcgcgcgct cgaaccgcac ccgctgtccg
     3601 gtcgccgggt gggtgaagcc gagcaccgcc gcgtgcagcg cgagccgggg cgcacgcggc
     3661 acgaacgtcc gcgccgtctc gccgccgtgc tggtggtcgc cggccaccgg gtggccgatc
     3721 cccgccaggt ggatccggat ctggtggctg cgcccggtct ccagccgcgc cgacaccagc
     3781 gtcgcgccag cgaggtgctc gattgcaccg atgtgagtca ccgcgcgctt gccctggatc
     3841 ggccggtcga gggtctgcgg cgccacgtcg ccgaccgcga ccgcacggta ctcgcggtcg
     3901 acgtcgtggc gcgcgaacgc ctcacccagc cgcttgttgg cctcgcgggt gcgggcgaac
     3961 acgagcaggc cgctggtcgg caggtcgagg cggtgcacca ggtagacctc gccgtggcgc
     4021 cgcgcgagct ggtcgagcag gtcgccgcgg tcgctctccg gggtcggcgc ggtgaccagg
     4081 cccgcgggct tgtcggcgac gatcacgtgg tcgtcgacga acacgatccc gggctccggg
     4141 ggtggccggt cgcggtcgag cgcgccgccg acgtggacct cgatcacctg gccggcgcgg
     4201 accggacgcc cggcgacctt gacccgggcg cggtcgacga acacgccgcc caggtcgatc
     4261 accgcgcgcg cctggcggcg cgacaggccg gcgacgtgcc ggggcacgac ctggtcgagc
     4321 cgtaggccgg cctcatcggc cgcgacttcg aacctcgtgc ccatggccta cggagctgcc
     4381 gcggtggtca ccgcgatccc tgcgcatcgc tgcccatcgc tgcccatcgc tggatgccag
     4441 cgccggctac cagtcgtcgc cgtcgtcgat gttgcggcgg cgcttcttgc gatcgtcctc
     4501 gccgccgccg cgctccttct ccttgtcctg ggccttccgg cggcggtggc ggtggacccg
     4561 gcggcgaccg ccgcggcagc tcctaggcca tgggcacgaa gttccaagtc gcggccgatg
     4621 aggccggcct acggctcgac caggtcgtgc cccggcacgt cgctggcctg tcgcgccgcc
     4681 aggcgcgcgc ggtgatcgat ctgggcggcg tgttcgtcga ccgcgcccgg gtcaaggtcg
     4741 ccgggcgtcc ggtccg
//
LOCUS       DASVOR010001852         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_398643,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001852 DASVOR010000000
VERSION     DASVOR010001852.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398643"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1815
                     /locus_tag="VF516_34430"
     CDS             <1..1815
                     /locus_tag="VF516_34430"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8112896.1"
                     /translation="VSAAQVRLDRIAARGCMAGGDRATVPAMLRTALLCSLLAACSGA
                     APSARPAAGGAAAPSAADQTFGRFVDEFFDASARFSPTPAVGQGFHAHDAEIEDRSAE
                     RIGQRIAELHAFQDRLARIDRGALGFDNAIDAEALAARIQGDLLSLETLKLWARNPMS
                     YAGLPGGAIDALMKRDFAPKHDRLRSMVARLRGVPAIFAAARANVVDPPKEFTDVAIA
                     RAKGTGGFLAGAVATWARDAAGDDAALLAEFDAASTAAIAASRSFADWLEHDLLPRSH
                     GSYALGEARFLELLRDDEMVELPLADLLAKGEANLRKDHDAFVATARLIDPKRTASQV
                     MTLLGDTHPTADDLIAAVGRSLEAARQFVVDKDLVTIPSEVRPRVEPTPPYARGTGFA
                     SMDTPGAFETRATEAFYYVEPVEPEWDAKHKDEHLRLFNPSVVALINVHEAFPGHYVQ
                     FLYSPRFPTKTRKLVGAGSNAEGWAHYAEQMMVDQGFGNGDPRIRLAQLEEALLRDCR
                     YVVGIKLHTQGMTVADGAKLFVDQCFQEPAVAYVEARRGTFNPTYLYYTLGKLEIQEL
                     AADYMRDKHATLKQFHDAFIAQGALPIPLVRRILLGRQ"
     gene            2018..2209
                     /locus_tag="VF516_34435"
     CDS             2018..2209
                     /locus_tag="VF516_34435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112897.1"
                     /translation="MKKQAGKKLTFSTETLRSLTSPTELEQVAGGQTTAVTCGCSLGC
                     TTAITCGQTAGCTTAIICG"
     gene            complement(2247..>2542)
                     /locus_tag="VF516_34440"
     CDS             complement(2247..>2542)
                     /locus_tag="VF516_34440"
                     /inference="COORDINATES: protein motif:HMM:NF025394.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lantibiotic dehydratase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8112898.1"
                     /translation="QRTSLEAILDPAGDPTGDLAAPLAVLHGRSTRLAPWVHELRGRE
                     RAGQLTQTLAELAWSFVHMFTNRMLRSASLAQELVLYDWLRRAYQSAIARERR"
BASE COUNT          398 a          934 c          866 g          344 t
ORIGIN      
        1 gtatcagcag cgcaggtgag gctcgatcgg atcgccgcgc gcggctgcat ggccggcggc
       61 gatcgcgcta ctgtgccggc catgttgcgc accgccctgc tgtgtagctt gctcgccgcg
      121 tgcagcggcg cggcgccgtc ggccagacct gccgccggcg gcgccgccgc accgtccgcc
      181 gccgaccaga ccttcggtcg cttcgtcgac gagttcttcg acgccagtgc ccggttctcg
      241 ccgacccccg cggtcggcca gggcttccac gcccacgacg cagagatcga ggaccgatcg
      301 gccgaacgga tcggccagcg aatcgccgag ctccacgcgt tccaggatcg gctggcccgg
      361 atcgaccgcg gcgcgctcgg cttcgacaac gcgatcgacg ccgaggcgct cgcggcgcgg
      421 atccagggcg acctgctgtc gctcgagacc ctcaagctgt gggccaggaa cccgatgagc
      481 tacgccggcc tgcccggcgg tgcgatcgac gcgctgatga agcgcgactt cgcgcccaag
      541 cacgaccggc tgcgctcgat ggtcgcccgg ctgcgcggcg tgccggcgat cttcgcggcc
      601 gcgcgcgcca acgtcgtcga tccgccgaag gagttcaccg acgtggcgat cgccagggcc
      661 aagggcaccg gcggcttcct cgccggcgcg gtcgcgacct gggcgcgcga cgcggccggg
      721 gacgacgccg ccctgctcgc cgagttcgac gccgccagca ccgcggcgat cgcggcgagc
      781 cgcagcttcg ccgactggct ggagcacgac ctgctgccgc gctcccacgg cagctacgcg
      841 ctcggcgagg cgcgcttcct cgagctgctc cgggacgacg agatggtcga gctgccgctc
      901 gccgacctgc tggccaaggg cgaggccaac ctccggaagg accacgatgc gttcgtcgca
      961 acggccaggt tgatcgatcc gaagcgcacc gcgtcgcagg tgatgacgct gctcggcgac
     1021 acccacccga ccgccgacga cctgatcgcc gcggtcggcc gcagcctcga ggcggcgcgc
     1081 cagttcgtcg tcgacaagga cctggtgacg atcccgtcgg aggtccggcc gcgggtcgag
     1141 ccgacgccgc cctacgcgcg cggcaccggg ttcgcgtcga tggacacgcc gggcgcgttc
     1201 gagacccgcg cgaccgaggc gttctactac gtcgagccgg tcgagcccga gtgggacgcc
     1261 aagcacaagg acgagcacct gcggctgttc aatccgtcgg tggtcgcgct gatcaacgtc
     1321 cacgaggcgt tccccggcca ctacgtgcag ttcctctaca gcccccggtt cccgaccaag
     1381 acccgcaagc tggtcggcgc gggcagcaac gccgagggct gggcgcacta cgccgagcag
     1441 atgatggtcg atcagggctt cggcaacggc gatcccagga tccggctggc ccagctcgag
     1501 gaggcgctgc tccgcgactg ccgctacgtg gtcggcatca agctccacac ccagggcatg
     1561 accgtcgccg acggcgccaa gctgttcgtc gatcaatgct tccaggagcc ggcggtggcg
     1621 tacgtcgagg cccgccgcgg cacgttcaac ccgacctacc tgtactacac gctcggcaag
     1681 ctcgagatcc aggagctcgc cgccgactac atgcgcgaca agcacgcgac gctcaagcag
     1741 tttcacgacg cgttcatcgc ccagggcgcg ctgccgattc ccctggtccg ccggatcctg
     1801 ctcggcaggc agtagccagc acggcccgag cacagactgc cgatcacgga gtcgcgcgcg
     1861 agcgaccggc gcggtgagcg ctgctcagcc tccgtgatcg cggacgtgcc cacgctcgcc
     1921 tgcgcggttt cgcgtagttc tgcccggagt gagagaatca cgggtactgt gacgttcaca
     1981 acggctggaa cgccagcgtg aagcgtagaa tcgagccatg aagaagcaag caggcaagaa
     2041 gctcacgttc tcgaccgaga cgctgcgctc gctgacgtcc cccaccgagc tcgagcaggt
     2101 cgccggcggc cagaccacgg cggtcacgtg cggctgttca ctgggttgca ccaccgccat
     2161 cacgtgcggt caaacggcgg gctgcaccac cgcgatcatc tgcggctgaa acccgctcgt
     2221 ctgccggcaa acgcggcagc gatgcctcac cgccgctcac gggcgatcgc ggactggtac
     2281 gcgcggcgaa gccagtcata cagcaccagc tcctgggcga gcgacgccga gcgcagcatg
     2341 cggttggtga acatgtggac gaagctccac gccagctccg cgagggtctg cgtgagctgc
     2401 cccgcgcgct cgcgcccgcg aagctcgtgc acccacggcg ccagcctcgt cgagcgtccg
     2461 tgcagcaccg cgagcggcgc agcgaggtcg cccgtcggat cgccggccgg gtcgaggatc
     2521 gcctccagcg acgtgcgctg cg
//
LOCUS       DASVOR010001853         4872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_312002,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001853 DASVOR010000000
VERSION     DASVOR010001853.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312002"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..836)
                     /locus_tag="VF516_34445"
     CDS             complement(135..836)
                     /locus_tag="VF516_34445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112899.1"
                     /translation="MSDAELEQLALIRHIVTVPQASPAQEQAFDRLVAMILPELDRYT
                     RRPYLPDDEADAVAIRVIAKLRHNNFKNLRAFLDRFDGDPTWRFEAWLRKLCRWKISH
                     RLDQLQRSGLLALDDDPSGALEQEVESTTLVIGTLIRSEHAELGRCLLERAAVGLDER
                     QCIALHLYYSRTEDAPEGRRQASTRAAYAEIAAQLELDVAEAIRLVTTAKQRLERAVR
                     RLREDERSDEAEAWA"
     gene            complement(980..1879)
                     /locus_tag="VF516_34450"
     CDS             complement(980..1879)
                     /locus_tag="VF516_34450"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEX8112900.1"
                     /translation="MPANAVMTYVLAMIVSGSIGLFVTESGLPAACVVFARCAIGAVA
                     VFMYCVWSGSFRGLHYTRRMLVLTILGGLCIVANWVMFFTAFSYTTITITTVVYHIQP
                     FVLAMMGWAVFGDRVTLDQAGWMAVAFGGIVLLTGVGPVAFGPELCAGVGLALAASVL
                     YSAASIIAKQLRAVPPQVTLVIQLAVGALATLPLASPGGVALAPRPVVCVVALGVVHT
                     GALYVLIYRALQRLPTTMIAMLTFIYPGVAVVLDVIYYGHVPGPPQVIGITMILVANV
                     ANARKWRFWLRTARPPADLQARV"
     gene            complement(1869..2948)
                     /locus_tag="VF516_34455"
     CDS             complement(1869..2948)
                     /locus_tag="VF516_34455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEX8112901.1"
                     /translation="MNAPLGPSEGNAIRQTYLVFPRWTQPLWTWWTGRALPGEQPLWK
                     PGPWGYLIGTLAAFAGGMALTAFALATWWPLAVAGWLITVGATRTLVSTICHQCVHYR
                     FTGKQRLDMQIADALAFVTFTKAAPIYRLEHTLQHHRGDVFTTARDPSAQFLTTCGLA
                     PGRTSGPQAARLLVNLVSPVFHARFLWSRIRGNLIDVGFWRRLVTGAAWIAALAALAR
                     ADALGEFALIYGVPVILLYHVSVMLEFASEHAWLVPRAPDSPASWGRFCGAPVPQRGI
                     AAWLAWWIAMIGYHLVVRVAVLPADLPQHDFHHRFPATKLWTHAAHARTACLDRSDVR
                     LVELWGLHRALGHVLRGLEELRHAG"
     gene            complement(2945..3799)
                     /locus_tag="VF516_34460"
     CDS             complement(2945..3799)
                     /locus_tag="VF516_34460"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8112902.1"
                     /translation="MRSFPLGLIDLDDARLAPELAAVLACNVTSDAYSEFKLGSWRTV
                     VLWNTSGQDGDGLIASGDVPARPTPRAQPLGYLDELIARTFDVSRLRLVRAHILEDGV
                     LIPHRDFLELAPDEPRWCRLHLPIATTTACLHSEEDTVFHMRRGEVWFLDASQTHSAC
                     NNGTTPRVTLCLDFALGDRPIESLVADRHQLRPTSPVVIARDPLDRPFRAGIRDLARL
                     INRHNYREILNFLARVHFYKAVDINEFFAWIVEVASAAGDRELTARLDGFGRFLRSDR
                     EMHERYAL"
     gene            complement(3810..4583)
                     /locus_tag="VF516_34465"
     CDS             complement(3810..4583)
                     /locus_tag="VF516_34465"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8112903.1"
                     /translation="MATALSSIPQPIAADHHASSRAFLTDLASTVYRSKALNNAFYDA
                     WTSRALPIEDIAIAVRNYGYFVRSFPEILATMIMSADNVVARTEYAKTLFSEMGYGQL
                     RAVHSVLLDKFFSELSAKLGDPDRLTQAGLAAMPLLPETAALVDGEKALYSSNSARAA
                     GAQLALEWQAYTMLRKLYDGACNYRALWSDEDEFHGACEYFYAHIGAAEKEHKLESLN
                     GAVELDADPAAREQIVLGFHEHLALFDRFWNAIAAAFSR"
BASE COUNT          757 a         1682 c         1763 g          670 t
ORIGIN      
        1 tggtcgagct cgcgccgccg ccgcccggcg aggtcatcgt cagccaggac aacgcggcgc
       61 ggttccgggc ctggctcgcc gggtagcgag gtcggcggcg atcacggcga tcggcctcgc
      121 cgtgggctgc cggatcacgc ccaggcctcg gcctcgtcgc tgcgctcgtc ctcgcgcagc
      181 cggcggaccg cgcgctccag ccgctgcttg gcggtggtga ccagccggat cgcctcggcg
      241 acgtcgagct ccagctgggc cgcgatctcg gcgtaggcgg cgcgcgtgct ggcctggcgc
      301 cggccctcgg gcgcgtcctc ggtgcgcgag tagtagaggt ggagcgcgat gcactggcgc
      361 tcgtcgagcc cgaccgcggc gcgctccagc aggcagcggc cgagctcggc gtgctccgag
      421 cggatcagcg tgccgatcac cagcgtggtc gactcgacct cctgctccag ggccccggac
      481 ggatcgtcgt cgagcgcgag caggccgctg cgctgcagct gatcgagccg gtgcgagatc
      541 ttccaccggc acagcttgcg cagccaggcc tcgaaccgcc aggtcgggtc gccgtcgaac
      601 cggtcgagga acgcgcgcag gttcttgaag ttgttgtggc gcagcttcgc gatcacgcgg
      661 atcgcgacgg cgtcggcctc gtcgtccggg aggtacggcc ggcgggtgta gcgatcgagc
      721 tccggcagga tcatcgcgac cagccgatcg aacgcctgct cctgcgccgg gctcgcctgc
      781 gggacggtca cgatgtggcg gatcagcgcc agctgctcga gctcggcgtc cgacatcgaa
      841 cgcgtcgcgg cacgaagcgg gcgggtcgcg gccaccatcg tcttctcgaa cgtgacgcct
      901 tcatttctac acagcgccgc gtgtatttcg atcgtggtag tagcgctttt gaccaggacg
      961 ctggcgtcga gtacggcttt tacacacgag cctgcaagtc cgcgggcggc cgcgcagtcc
     1021 tgagccagaa ccgccatttg cgggcattgg cgacattggc cacgaggatc atcgtgatgc
     1081 cgattacctg aggtggaccc gggacatggc catagtagat gacgtcgagc accaccgcga
     1141 cccccgggta gatgaaggtc agcatcgcga tcatcgtggt cggcagtcgc tgcagcgcgc
     1201 gatagatcag cacgtagagc gccccggtgt ggaccacgcc gagcgcgacg acgcacacca
     1261 ccgggcgcgg cgcgagcgcg acgccgccgg gcgacgccag cggcagggtg gcgagcgcgc
     1321 cgaccgccag ctggatcacc agggtgacct ggggcgggac cgcgcgcagc tgcttggcga
     1381 tgatcgacgc ggccgagtac agcaccgagg cggcgagcgc gagcccgacg ccggcgcaca
     1441 gctcggggcc gaacgcgacc ggcccgacgc cggtcagcag cacgatgccg ccgaacgcga
     1501 cagccatcca gcccgcctgg tcgagcgtca cccggtcgcc gaacaccgcc cagcccatca
     1561 tcgcgaggac gaacggctgg atatgataga ccaccgtcgt gatcgtgatc gtggtgtacg
     1621 agaacgcggt gaagaacatc acccagttgg cgacgatgca cagcccgccg aggatcgtga
     1681 gcaccagcat gcgccgggtg taatgcaggc cacgaaagct ccccgaccac acgcaataca
     1741 tgaatactgc gaccgcgccg atcgcacagc gcgcgaacac gacgcaggcc gccggcagtc
     1801 cggactcggt gacgaacagc ccgatcgatc cggacacgat catggcgagc acgtaggtca
     1861 tcacggcgtt agccggcatg gcggagctcc tcgaggccgc gcagcacgtg gccgagcgcg
     1921 cggtgcaagc cccacagctc gaccagccgc acgtcgctgc ggtcgaggca cgcggtgcgg
     1981 gcgtgcgcgg cgtgggtcca cagcttggtc gcggggaagc ggtggtggaa gtcgtgctgc
     2041 ggcaggtcgg cgggcagcac cgcgacccgg acgaccaggt gatagccgat catcgcgatc
     2101 caccaggcga gccaggccgc gatgccgcgc tgcggcaccg gggcgccgca gaaccgaccc
     2161 cagctcgccg ggctatcggg ggcgcgcggc accagccacg cgtgctcgct ggcgaactcc
     2221 agcatgacgc tgacatggta gagcaggatc accggcacgc cgtagatcag cgcgaactcc
     2281 ccgagcgcat ccgcacgggc gagcgccgcg agcgccgcga tccacgccgc gccggtcacc
     2341 agccgccgcc agaacccgac gtcgatcagg ttgccgcgga tccggctcca caggaaccgg
     2401 gcgtggaaca ccggcgacac caggttgacc agcagccggg cggcctgggg cccgctcgtc
     2461 cggcccggcg cgaggccaca ggtcgtgagg aactgcgcgc tgggatcgcg ggcggtcgtg
     2521 aacacgtcgc cgcggtggtg ctggagcgtg tgctccaggc ggtagatcgg cgccgccttg
     2581 gtgaacgtga cgaacgccag cgcgtcggcg atctgcatgt cgagccgctg cttgccggtg
     2641 aaccggtaat gcacgcactg gtggcagatc gtgctgacca gcgtgcgggt cgcgccgacc
     2701 gtgatcagcc agccggcgac cgccagcggc caccacgtcg ccagcgcgaa cgcggtcagc
     2761 gccatcccgc cggcgaacgc cgcgagcgtg ccgatcaggt agccccacgg cccgggcttc
     2821 cacagcggct gctcgccggg cagcgcgcgg ccggtccacc acgtccacag cggctgggtc
     2881 cagcgcggga acaccaggta ggtctgccgg atcgcgttgc cctcggatgg tccgagcggc
     2941 gcgttcacaa cgcgtacctc tcgtgcatct cgcggtcgct gcgcaggaac cggccgaacc
     3001 cgtcgagccg ggcggtgagc tcgcggtcgc cggcggcgct cgcgacctcg acgatccagg
     3061 cgaagaactc gttgatgtcg accgccttgt agaagtggac gcgcgccagg aagttcagga
     3121 tctcgcggta gttgtggcgg ttgatcagcc gggcgaggtc gcggatcccg gcgcggaacg
     3181 ggcggtcgag cggatcgcgc gcgatcacga ccggcgaggt cggccgcagc tggtggcggt
     3241 cggccaccag ggactcgatc ggacggtcgc cgagcgcgaa gtcgaggcac agcgtcaccc
     3301 gcggggtcgt cccgttgttg cacgcggagt gggtctggga cgcgtcgagg aaccacacct
     3361 cgccgcgccg catgtggaac acggtgtcct cctcggagtg caggcacgcc gtggtggtcg
     3421 cgatcggcag gtggagccgg caccagcgcg gctcgtcggg cgcgagctcc aggaagtcgc
     3481 ggtgcgggat cagcaccccg tcctccagga tgtgggcgcg caccagccgc agccgggaca
     3541 cgtcgaacgt ccgcgcgatc agctcgtcga gatagccgag cggctgggcg cggggcgtcg
     3601 gccgcgcggg gacgtcgccg ctcgcgatca gcccgtcccc gtcctgcccc gaggtgttcc
     3661 acagcaccac ggtgcgccag cttccgagct tgaactccga gtacgcgtcg ctggtcacgt
     3721 tgcacgccag caccgcggcg agctcgggcg cgagccgggc gtcgtcgagg tcgatcaggc
     3781 ccagggggaa cgagcgcatg gcggcctcct caccggctga acgccgcggc gatcgcgttc
     3841 cagaaccgat cgaacagcgc gaggtgctcg tggaacccca gcacgatctg ctcgcgcgcc
     3901 gcgggatcgg cgtcgagctc gaccgcgccg ttgagcgact ccagcttgtg ctccttctcg
     3961 gccgcgccga tgtgggcgta gaagtactcg cacgcgccgt ggaactcgtc ctcgtcgctc
     4021 cacagcgcgc ggtagttgca tgcgccgtcg tacagcttgc gcagcatggt gtaggcctgc
     4081 cactccagcg cgagctgggc gccggcggcg cgcgcgctgt tgctcgagta cagcgccttc
     4141 tcgccgtcga ccagcgcggc cgtctcgggc agcagcggca tcgcggccag cccggcctgg
     4201 gtcagccggt ccgggtcgcc gagcttggcc gacagctcgg agaagaactt gtcgagcagc
     4261 accgagtgga ccgcgcggag ctggccgtac cccatctcgg agaacagcgt cttggcgtac
     4321 tcggtgcgcg ccaccacgtt gtcggccgac atgatcatgg tcgccaggat ctcggggaac
     4381 gaccgcacga agtagccgta gttgcggacc gcgatcgcaa tgtcttcgat cgggagcgcc
     4441 cgcgacgtcc acgcgtcgta gaatgcgttg ttgagcgcct tgctgcggta gaccgtggac
     4501 gccaggtcgg tcaggaatgc gcgcgacgag gcgtggtgat ctgcagcgat cggctgcggg
     4561 atgctcgaga gtgcagtggc catcggatgc tccttgtcgt cggtcgctat ccgcgaacgc
     4621 cggagatccg gaatccacac agcaattttg acagccggtc atgccgcgct gcgcggtggt
     4681 ggcgcaccgc gcgctcgtgg atcacgatgc cgccatgcgc ggttcgcgat gcagccgcgg
     4741 ctcaggaccg gcgcgccggc cgggtccgcc gcagctcgac cagctcgccg gcgatctgcg
     4801 cctgctggcg ccactggcca taggccgcgc cgcgcgactc cagccgggcg atcgcggcgg
     4861 cgacctcggc gg
//
LOCUS       DASVOR010001854         4232 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_370197,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001854 DASVOR010000000
VERSION     DASVOR010001854.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4232
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370197"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(741..1307)
                     /locus_tag="VF516_34470"
     CDS             complement(741..1307)
                     /locus_tag="VF516_34470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112904.1"
                     /translation="MSQVDEWLPADGGVGGQEAGVDQSVDDRVSGSVLGTAGCQRRAR
                     LLPPGVRAALAEADQAQEDPLGDALLGGREVMQRGVGAPRQRDGDSAACLARPRIAAG
                     RRLGSRRTQIVRVRIERLVARQPEREARQTSWRIGDTAAQDPTRIRPLSSHLGRRGAE
                     SGLLTWPPVNGPVSACGDVADSRPGTGR"
     gene            1660..3027
                     /locus_tag="VF516_34475"
     CDS             1660..3027
                     /locus_tag="VF516_34475"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8112905.1"
                     /translation="MIALALIAIVASSFGLVAWRQCQEAQRQLARNELEQARSLVVEG
                     RNSPGAIPHLIEATRLGIDTPVLRTLLAAAGQDLWRFSLPYGGRVHSATFSPDGTRVV
                     TASLDGTARVWDATTGNPLGVSLKHEEAVRSAAFSLDGTRVVTASSDGTARIWQIAET
                     GPLDAWIQEATSCLAQPKEAVGSSRVCQLTKRPATTEDLVTRAQALVAAGDREMHTHV
                     WATPRARYLQALTLLDPSGSSAPPQDLIVEFRRSISVRLAILAAMEGRVAEARTWLGP
                     NAPPKDWSLLDGLGSVAHDGLHEEIVAVALLRSAHEQDSGDPDILADLAEMYFAAGQI
                     EQCAHAAAQIDQRRASLEVRVAVAALRCAALGTRPTDPHTETTRDADRLFHVYREVAT
                     RTRLLWTWNGTKHALTYGRYRFEDMKPIVEVLTLLEQPVTDETRAQLARLLAAPVPSH
                     SNPPR"
     gene            3171..>4232
                     /locus_tag="VF516_34480"
     CDS             3171..>4232
                     /locus_tag="VF516_34480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112906.1"
                     /translation="MADDTLQTQTVDPLEARVVVFRQQPDARGFAELRQELRDAGRGE
                     LLADVCATWAPHERDPIRAADAWSEAGEAMVVLGEAETAVEYLQQAIDLDPTNDRAID
                     RLLEIVEPGDPAAAAEVIEHELTELAQLFDPARKRPGLDGRRKTDVIARRAAQHRRAA
                     GLWDQVGRVDRALWHYQQAWKLEPHRTEALEAARQLYHSLGDDAMVGKLFGAELEVIG
                     GDRSPAAAARKAQIRLELGKLALRGKDLETAAGHLEEAARLDPTSLEVAEKLAEVYAT
                     PGFRGGDDGAWRHKASQLVVEVGRRRMASRDDSTAIKYLRRAAGIDPYSKGSSVALEQ
                     ALSEASQWQELDRMLRHRSN"
BASE COUNT          643 a         1506 c         1496 g          587 t
ORIGIN      
        1 cctccttacg atctcgcaag actcgcttcc ggcgtggcgg tctccgtcgt cgccggtggg
       61 attttcaccc cgggtcgctc tccgaaattt cgggctgcta catgccttcc tttccggcca
      121 ggcttgtcct ggcgccctcg cagcgtccga ccccatcctg ctcgcggtcg cccccggcga
      181 ggtcgcgttg acgtctggcg atcgcgccac gcggcgaaca tcgacggtcg attccggaac
      241 gacagcggaa gccggtcttg acctaagaag cgcctcaagc caggcgattc cggggaatcg
      301 tggcggcgcc ctcggcggct gacatcgcgg aacgcgatgc gggcaagcgc caggccaggg
      361 cccatcgcgg gcacccacgg ggggcgttgg cgtccgcggt tggctgcgcg gtgctcggac
      421 gattccgcgg aatcgctgtc ggctccctcg acggctgcat ggcgaccgcg gtacaggcaa
      481 gcgccggctc gctacaccgc gctcgaagcc caagcgccca gatgccgcgc cggatcccgg
      541 cggcgaggtc gtcggcggag acgttctcca gctcgccgcc caagcgcgca cacacatgac
      601 ctatgtgctc gagagggtcg cctaccaggc cgagcgcgac gccgtccgcc gcaccctcgg
      661 cgtgccgatg gcgaggcagg tacgctcgct tgccaatcgg cgctcaccaa cgccacagcg
      721 gtcaccgcga ccgcgacgcg tcaccttcca gtacccggcc gcgaatctgc cacgtcgcca
      781 caggctgaaa ccggaccgtt cacgggtggc catgtcagga gtccggattc agcgccgcgt
      841 ctgcccagat gtgacgacag cgggcgaatc ctggtcggat cttgcgctgc cgtgtcgcct
      901 atccgccatg acgtctgtct cgcctcccgc tccggctggc gagctacgag gcgctcgatg
      961 cggacgcgca cgatctgggt gcgcctcgaa ccgaggcgcc ggccagccgc gatgcgcggc
     1021 cgcgcgaggc acgcagcgga atcgccgtcg cgctggcgag gcgcgccgac gccgcgctgc
     1081 atgacctcac gcccgccgag cagcgcgtcg ccgagcggat cctcctgcgc ctggtcagct
     1141 tcggcgaggg ccgccctgac acccggcggc agcagccgcg cgcggcgctg gcagcctgcg
     1201 gtaccgagga cgctgccgct gaccctgtca tcgaccgact ggtcgacgcc cgcctcctga
     1261 cctccgacgc caccgtcggc ggggagccac tcgtcgacct ggctcacgag gctgatcgcc
     1321 ggctggcccc agctccagca ctggctctgg tcgcgccggg acgacgagca gcgccggcag
     1381 ctgctcgagc tgcatgcggc gcaatgggtc cagcgcgggc gcggcagtgt cggcctgttc
     1441 gatgcgatcg agctcgccga ggccgagcgc tggctcgccg gcgacaccgc gcgcgagctc
     1501 ggctacagcg acgacctgcc gcggttcctg gccgccaacc gcgacgcgat cgccgaggcc
     1561 gagcgccagc gccagctcgc cgcggagctg caggagcgag aacggcgcgc cgcccacgag
     1621 gagcgcaccc ggctgcgccg gcggctcacc cggttcacca tgatcgccct ggcgctgatc
     1681 gcgatcgtcg cgtcgagttt tggcctcgtg gcctggcgcc agtgccagga agcacagcgc
     1741 caactcgcgc gcaacgaact ggaacaagcg aggagcctcg tggtcgaggg ccgcaactca
     1801 cccggcgcga ttccacatct catcgaggcg acccggctgg gcattgatac accggtgctg
     1861 cgcacgctgc tcgcagccgc ggggcaagac ctgtggaggt tctcgcttcc ctatggagga
     1921 cgggtccata gcgccacatt cagccccgac gggacgcgcg tggtgaccgc cagcctagat
     1981 ggcaccgcgc gggtgtggga cgcgacgacc ggcaacccgc tgggcgtctc gctcaagcac
     2041 gaggaggcgg tccggagcgc cgcgttcagc ctcgatggca cgcgtgtggt caccgccagc
     2101 agcgatggca ccgcgcggat atggcagatc gcggagaccg gcccgctgga tgcgtggata
     2161 caggaagcga cgagctgtct cgcgcaaccg aaggaagcgg tcggatcgtc tcgcgtctgt
     2221 cagctcacca agcggcctgc gaccacggag gatctggtga cgcgcgcgca agcactcgta
     2281 gccgctggcg atcgcgagat gcacacacac gtctgggcta ccccgcgagc gcgttacctg
     2341 caggcgctca cgttgctcga tccgtcgggt agctccgcgc cgccgcaaga tctaatcgtg
     2401 gagtttcggc gatcgatatc ggtgcgactg gcgattctcg ctgcgatgga aggtcgcgtg
     2461 gcggaggccc gcacctggct cggaccgaac gctcccccaa aggattggtc tctactcgat
     2521 ggactcggca gcgtcgctca cgacgggcta cacgaggaga tcgtcgctgt ggcgttgctt
     2581 cgtagcgcgc acgaacaaga ttcgggcgat ccggacattc ttgctgatct cgctgaaatg
     2641 tacttcgcag ccggacagat cgaacagtgc gcacacgcgg ctgctcagat tgaccagcgg
     2701 cgggcgagcc tggaggtgcg cgtcgcggtc gccgcgctgc gctgcgctgc gctgggcacg
     2761 cgaccgactg acccacacac ggagaccacc agggacgctg accgactgtt tcatgtctat
     2821 cgcgaggtcg caacccgcac gcgccttctg tggacgtgga acggcaccaa gcatgcgctc
     2881 acgtacggac gctaccgttt cgaggacatg aagcccattg tcgaggtgct cacgctgctg
     2941 gagcagccgg tcaccgacga gacccgcgcg cagctcgcca ggctgctcgc agcgcccgtg
     3001 ccctcgcact cgaacccgcc gcgctgagct cgctacaacc tgcgcgccct gaggcgacgg
     3061 cgcgcagccc accaggccgc gccggcgtcc gaattctgcg cagctcgcct ccaagccccc
     3121 gccgggccac gcgatctcgg gctgcgtggt atcgtcggcg accgggaccc gtggccgacg
     3181 acacgctgca gacccagacc gtcgatccgc tcgaggcgcg ggtggtcgtg tttcgccagc
     3241 agcccgacgc ccgcggcttc gcggagctgc gccaggagct gcgcgacgcc ggccgcggcg
     3301 agctgctggc cgacgtgtgc gcgacctggg cgccccacga gcgcgatccg atccgcgcgg
     3361 cggatgcgtg gtcggaggcc ggcgaggcga tggtggtgct cggcgaggcc gagaccgcgg
     3421 tcgagtacct gcagcaggcg atcgacctcg atccgaccaa cgaccgggcg atcgaccggc
     3481 tgctggagat cgtcgagccc ggtgatccgg cggccgcggc cgaggtgatc gagcacgagc
     3541 tcaccgagct ggcgcagctg ttcgatccgg cgcgcaagcg gccggggctc gacgggcggc
     3601 gcaagaccga cgtgatcgcg cggcgggcgg cgcagcaccg gcgggccgcc gggctgtggg
     3661 atcaggtcgg acgggtcgac cgcgcgctgt ggcactacca gcaggcctgg aagctcgagc
     3721 cgcaccgcac cgaggcgctg gaggcggctc gccagctcta ccactcgctc ggcgacgacg
     3781 cgatggtcgg caagctgttc ggcgccgagc tcgaggtgat cggcggcgac cggtcgccgg
     3841 cggcggcggc gcgcaaggcg cagatccggc tcgagctcgg caagctcgcg ctgcgcggca
     3901 aggatctcga gacggcggcc gggcacctcg aggaagcggc gcggctcgac ccgacgtcgc
     3961 tcgaggtcgc cgagaagctc gccgaggtct acgcgacgcc cgggttccgc ggcggcgacg
     4021 acggcgcgtg gcgccacaag gcgagccagc tggtcgtcga ggtcggccgg cggcggatgg
     4081 cgtcgcgcga cgactcgacc gcgatcaagt acctgcggcg cgctgcgggc atcgatccgt
     4141 actccaaggg cagctcggtg gcgctggagc aggcgctgtc ggaggcgtcg cagtggcagg
     4201 agctcgatcg catgctgcgc caccgcagca ac
//
LOCUS       DASVOR010001855         2491 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_281511,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001855 DASVOR010000000
VERSION     DASVOR010001855.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281511"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..1083
                     /locus_tag="VF516_34485"
     CDS             118..1083
                     /locus_tag="VF516_34485"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HEX8112907.1"
                     /translation="MKQLPRGSDALILLERLAAARPDAVRELARVEAMPIYAFRFGSV
                     APRAPVLLVTGGVHGLERIGTEVAIAFLSSLITRLGWDHVLHDALARCRVIVLPLVNP
                     IGMARGQRANGNGVDLMRNAPAHELGGTPLVGGHRISPRLPWYMGRAGAPMETEAAAL
                     VRLVEDELFVAPFAIALDLHSGFGFVDRLWYPYARTREPPPDLPALVALGRLLDDTLP
                     NHVYHFEQTAQTYTIRGDLWDYLYDRRRAVGGGVLLPLTLEMGSWTWVRKNPAQILSS
                     LGRFNPLKPHRHRRTLRRHLPLLDFLLHATASHHAWTPSPAPHDR"
     gene            complement(1167..1583)
                     /locus_tag="VF516_34490"
     CDS             complement(1167..1583)
                     /locus_tag="VF516_34490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112908.1"
                     /translation="MTKANTITYASNWLVPGIILPRGPGCNQITLTWDRTHPGTTGSC
                     HIDPNACGLDEFGDRTICTKMAVALSDMKLTLLKEKPGHFAYTMEWRPHGSAEPYYAV
                     PLRLVTIAAPGKELQVRLLVLKPDQTIERIIDLHKV"
     gene            complement(1814..>2491)
                     /locus_tag="VF516_34495"
     CDS             complement(1814..>2491)
                     /locus_tag="VF516_34495"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8112909.1"
                     /translation="LDEIGELPLELQPHLLRVLESRRVRRIGGASERTVDVRIIAATN
                     RLENLGTDASKLRLDLYHRLATVVLVLPPLRERMGDLSDLVQGMLAELAPEHGPKLLS
                     DAAWKALAENRWPGNVRELRHAVVRAVALGGDQLEAEHFFPELRLAWSPALDDVPDAE
                     PLVPYERVLRGAMERAIAAHGSIRAAASHLGMPKSTFADRAQRWGLLPRDRPRMPYSY
                     PRAKQLK"
BASE COUNT          308 a          932 c          880 g          371 t
ORIGIN      
        1 gctgtcgggc ggcgcctccg tgctgaccgc ggagggccgg ggctgcgcgc tcgccgtcga
       61 tcgcgatctg cacggcttcc gcgtgcgcct cgcgtcgcca gagcgcacgc tcccggcgtg
      121 aagcagttac cgcgcggctc cgacgcgctg atcctgctgg agcggctggc cgcggcccgg
      181 ccggacgcgg tccgcgagct cgcgcgggtc gaggcgatgc cgatctacgc gttccggttc
      241 ggcagcgtcg cgccgcgcgc gccggtgctg ctggtgaccg gcggcgtgca cggcctcgag
      301 cggatcggca ccgaggtcgc gatcgcgttc ctgtcgtcgc tgatcacccg gctcggctgg
      361 gaccacgtgc tgcacgacgc gctcgcgcgc tgccgcgtga tcgtcctgcc gctggtcaac
      421 ccgatcggca tggcgcgcgg ccagcgcgcg aacggcaacg gcgtcgacct gatgcgcaac
      481 gcgcccgccc acgagctcgg cggcacgccg ctggtcggcg gccaccggat ctcgccgcgg
      541 ctgccgtggt acatggggcg agcgggcgcg ccgatggaga ccgaggccgc cgcgctggtc
      601 cgcctggtcg aggacgagct gttcgtcgcg ccgttcgcga tcgcgctcga tctgcactcc
      661 gggttcggct tcgtcgaccg gctgtggtac ccgtacgcgc gcacccgcga gccgccgccc
      721 gatctgccgg cgctcgtcgc gctcggccgg ctgctcgacg acacgctgcc caaccacgtg
      781 taccacttcg agcagaccgc gcagacctac acgatccgcg gcgatctgtg ggattacctc
      841 tacgatcgcc ggcgcgcggt gggcggcggc gtgctgctgc cgctcaccct cgagatgggc
      901 tcgtggacct gggtgcgaaa gaatcccgcc cagatcctgt cctcgctcgg ccggttcaac
      961 ccgctcaagc cgcatcgcca ccgccgcacg ctgcgccgcc acctgccgct gctcgacttc
     1021 ctgctccacg cgaccgcctc gcaccatgcc tggacgccct cgccggcacc gcacgaccgg
     1081 tgaccggcca cagcgccggc gcaccacccg gacggagtcg cgaggccgcg atgaagcgat
     1141 caccggccgc ggtcggcgca cgcacctcac actttgtgca ggtcgatgat gcgctcgatc
     1201 gtctgatccg gcttgagcac cagcaggcgg acctgcagct ccttgcccgg tgcggcgatc
     1261 gtgaccaggc gcagcggcac cgcgtagtac ggctcggcgc tgccgtgtgg ccgccattcc
     1321 atggtgtacg cgaagtgccc gggcttctcc ttgagcagcg tgagcttcat gtcgctgagc
     1381 gccactgcca tcttcgtgca gatcgttcga tcgccgaact cgtcgagccc gcaggcgttc
     1441 ggatcgatgt ggcagctgcc ggtggttccc ggatgcgtgc gatcccaggt cagcgtgatc
     1501 tggttgcagc ccggtccgcg cggcaggatg atccccggta ccagccagtt cgacgcatac
     1561 gtgatggtgt ttgccttcgt catggcgcgg tcctcctcgg gccacagacc cactcgccac
     1621 cggcagctgc aaggccggta ccaggcacga cgtgcgttcg cgctcgcgcc ggccacggat
     1681 tccgcaaccg gtgcgggcat cgccggccga ggcgcacccc gttcgcgtgt ccgcgcgcgg
     1741 gggcccggtg cccgcgtccg ctgcacgtgc gcgcgtgctc ggcgagcggc cctcgtcatg
     1801 agcgcgccga tcgctacttg agctgcttcg cgcgcggata gctgtacggc atccgcggcc
     1861 gatcgcgcgg caatagcccc cagcgctgcg cgcggtcggc gaacgtcgac ttcggcatgc
     1921 cgaggtggct ggccgcggcg cggatcgagc cgtgcgccgc gatcgcgcgc tccatcgccc
     1981 cgcgcagcac gcgctcgtag ggcaccaggg gctcggcgtc cggcacgtcg tcgagcgccg
     2041 gcgaccatgc gagccgcagc tcggggaaga agtgctcggc ctccagctga tcgccgccga
     2101 gcgcgaccgc gcgaaccacc gcgtggcgca gctcgcgcac gttgcccggc cagcggttct
     2161 cggcgagcgc cttccacgcg gcgtccgaca gcagcttggg cccgtgctcg ggcgcgagct
     2221 cggcgagcat gccctgcacc aggtccgaca ggtcgcccat ccgctcgcgc agcggcggca
     2281 gcaccagcac cacggtggcc agccggtggt agaggtcgag ccggagcttg gacgcgtcgg
     2341 tcccgaggtt ctccagccgg ttggtcgcgg cgatgatccg gacgtcgacc gtgcgctcgc
     2401 tggcgccgcc gatccggcgg acccggcgcg actccagcac gcgcagcagg tggggctgga
     2461 gctcgagcgg cagctcgccg atctcgtcga g
//
LOCUS       DASVOR010001856         3795 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_575716,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001856 DASVOR010000000
VERSION     DASVOR010001856.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3795
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_575716"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            698..2170
                     /locus_tag="VF516_34500"
     CDS             698..2170
                     /locus_tag="VF516_34500"
                     /inference="COORDINATES: protein motif:HMM:NF014395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HEX8112910.1"
                     /translation="MLNRDDWYDIARDVEWTLSYVRQEDAFPAEWQGAADIPPEAWAA
                     WDEPYRATYREYVAIQRDKDAGVYAVKEAFKRSGAFERLDAGYQTVAILHFAGLAAIE
                     YAAVTLEARFARFAPTPRWRNIAIYGMLDEIRHTQLDLAFAHDLLKHSPQFDWTQKAY
                     HTNEWGILAVRHFFDDAMLGASCIDGAIATNLTVEHGFTNLQFVALAADAMAAGDVSF
                     SNLLSSIQTDEARHAQQGFPTLEILARHDRRRAQELLDVSFWRALRLFQVITGPAMDY
                     YTPLGHRKQSFKEFMLEWIVNHHERVLADYGLERPWYWETFLASLEHGHHAIHLGLWF
                     WRPTLFFHPSAGVSKAERAWLRDKYPSWEDSWGCMWDVIIDNVNAGRRALTYPETLPA
                     LCNLCQLALGSALDRHHVRPYITEHGGRRYQFCSEPCQWIFERTPERYAGHHNLVERF
                     IGGEITPMNLEGGLAYMGITPDVMGDDCHGYRWAADYRRD"
     gene            2173..2436
                     /locus_tag="VF516_34505"
     CDS             2173..2436
                     /locus_tag="VF516_34505"
                     /inference="COORDINATES: protein motif:HMM:NF017993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene-4-monooxygenase system B family protein"
                     /protein_id="HEX8112911.1"
                     /translation="MALFPIYGRFVGDCIPHLVAVDTGDTMAQVAEKIAVHSVGRRIR
                     PRPGGHGYEVLVGGHRVDGDAVLGSLGLHPLDWVDVRFAEGPE"
     gene            2440..2781
                     /locus_tag="VF516_34510"
     CDS             2440..2781
                     /locus_tag="VF516_34510"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8112912.1"
                     /translation="MAARFERLCDDGELWDGEMTAFDVSGEDVLLVRLDGCYHAYQGT
                     CPHQRVRLVEGSLDGATLTCRGHLWQFDARSGRGINPAASRLTRHAVKIEAGGVWVQT
                     VPEPDSGQGGA"
     gene            2778..3086
                     /locus_tag="VF516_34515"
     CDS             2778..3086
                     /locus_tag="VF516_34515"
                     /inference="COORDINATES: protein motif:HMM:NF014461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HEX8112913.1"
                     /translation="MTTEQATLVGPVIRAGEVADAVALAIEDDNPGRAVRVTDRGDYV
                     RIEVDGRCRLTRRSLEHHLGRELSLPQLEIEMPSFAGRLSTRTDEYVWYFENAGGGHD
                     "
     gene            3079..>3795
                     /locus_tag="VF516_34520"
     CDS             3079..>3795
                     /locus_tag="VF516_34520"
                     /inference="COORDINATES: protein motif:HMM:NF014395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toluene hydroxylase"
                     /protein_id="HEX8112914.1"
                     /translation="MTDDVADARARRRAQRLRTWSRLGHLGRVPTEYEIVTHGMNYTT
                     SSRTPLEAGPDTFGNRWIAEHRDAVAVRVDAWDDFRDPDELTYRKYNRCQDAAEAFVD
                     QVLEDYGERRDADRGHHPRWLYALGMLLTPQRYPVHGLQMVAAYVAQMAPSSYITNAA
                     TFQAADELRRVQRIAYRTRQLDLAHPALGFGGTERATFETHKRWQAARAAIEKLLVVF
                     DWDEAFTALELAIKPAYDEIY"
BASE COUNT          609 a         1306 c         1332 g          548 t
ORIGIN      
        1 cagaacctgc tcggcccccg gctcgccgcc tggctgacca acgcgccgcg ctgagcccgg
       61 cggcgacgtg gacgcgggcg gccggccgct gcacgcgcag ggcagtcacg agtccgcaag
      121 ggcctctgcg atgctcgcgc agtgaacggc tcgggcgaac cagcgctgca gccgctgcag
      181 atcgcgctcg gcgaggatgc aggccggaca ggcgacaggc gatctctccc agcgcctggg
      241 agctgggctc gtgtagcgaa ccctccgggc atgcacgatc tcggagcatg ggatcccgct
      301 tgcgcgcggg cgcacacccg ggggctgcca ccggatcgag aacggccgct tcgagattgc
      361 gatcggtaca ggcgacatgt ccgatttcca gacgctcgac tcacggcgcc gtccgttgcg
      421 atgcgcggtc gctgcaatcg agcagcggtc ctggattgca tcggtctgac gtaaggacac
      481 gaaggctgca ccgtcgacgc gatcgtgaaa tccagcaccg ggacgtgcgc gcgaagatga
      541 acgtgctacg aatctgctcg gtcatggctg agtggtgtgc ggacggcagt gagcgagccg
      601 gggagttgca ccgtgccggg tgcgatggcc ccgacgtgcg agccgcccgt actggttgac
      661 agagcgacgc gtctcgaccc agggggtgtt tgtggccgtg ctgaatcgtg acgattggta
      721 cgacatcgcg cgcgatgtcg aatggacgct gtcgtacgtg cggcaggagg acgcgtttcc
      781 cgccgagtgg cagggagcgg cagacatccc gcccgaggcg tgggctgcgt gggacgaacc
      841 ctaccgcgcg acgtatcgcg aatacgtcgc catccagcgc gacaaggacg cgggcgtgta
      901 cgcggtcaag gaggcgttca agcgatcggg cgcgttcgag cggctcgacg ccggatacca
      961 gacggtcgcg atcctgcact tcgcagggct cgccgcgatc gagtacgcgg cggtgacgct
     1021 cgaggcccgg ttcgcgcggt tcgcgccgac accgcggtgg cgaaacatcg cgatctacgg
     1081 gatgctcgac gagatccgcc acacccagct cgatctcgcg ttcgcgcacg atctgctgaa
     1141 gcactcgccg cagttcgact ggacccagaa ggcctaccac accaacgagt ggggcatcct
     1201 cgccgtgcgg cacttcttcg acgacgcgat gctcggcgcg agctgcatcg acggcgcgat
     1261 cgccaccaac ctcaccgtcg agcatggctt caccaacctg cagttcgtgg cgctcgcggc
     1321 cgatgcgatg gcggccggtg acgtcagctt ctcgaacctc ttgtcgagca tccagaccga
     1381 tgaggcccgg catgcccagc agggcttccc cacgctggag atcctggccc gccacgaccg
     1441 gcggcgcgcc caggaattgc tcgatgtcag cttctggcgc gcgctccggc tgttccaggt
     1501 gatcaccgga ccggcgatgg actactacac gccgctcggc catcgcaagc agtcgttcaa
     1561 ggagttcatg ctcgagtgga tcgtgaatca tcacgagcgg gtgctcgccg actacgggct
     1621 cgagcggccg tggtactggg agaccttcct ggcgagcctc gagcacggtc accatgcgat
     1681 ccacctcggg ctgtggttct ggcgaccgac gctgttcttc cacccgagcg ccggagtctc
     1741 gaaggccgag cgcgcgtggc tacgcgacaa gtatcccagc tgggaagaca gctggggctg
     1801 catgtgggat gtcatcatcg acaacgtcaa tgcgggccgg cgcgcgctca cctaccccga
     1861 gacgctgccc gcgctgtgca acctgtgcca gctcgcgctc ggctcggcgc tcgatcgcca
     1921 ccacgtccgg ccctacatca ccgagcacgg cggcaggcgt taccagttct gcagcgagcc
     1981 ctgccagtgg atcttcgagc gcacaccgga gcgctacgcc gggcaccaca acctcgtcga
     2041 gcggttcatc ggcggagaga tcaccccgat gaacctcgag ggcggcctcg cgtacatggg
     2101 catcacgccc gacgtgatgg gcgacgactg ccacggatac cgctgggcag ccgactacag
     2161 gagagactga ccatggcgct gttcccgatc tacggccggt tcgtcggcga ctgcatcccg
     2221 cacctggtgg cggtcgacac cggcgacacg atggcgcagg tcgccgagaa gatcgcggtg
     2281 cactcggtcg gccggcgcat ccggccccgg ccgggcggcc acggctacga ggtcctggtc
     2341 ggcggccacc gggtcgacgg cgacgccgtg ctgggctcgc tcggcctgca cccgctcgac
     2401 tgggtcgacg tgcggttcgc agagggcccg gagtgagcga tggccgcgcg gttcgagcgc
     2461 ctgtgcgacg acggcgagct gtgggacggc gagatgacgg cgttcgacgt ctcgggcgag
     2521 gatgtgctgc tggtccgcct cgacggctgc tatcacgcgt accagggcac ctgcccgcac
     2581 cagcgggtgc gcctggtcga gggctcgctc gacggcgcga cgttgacctg ccgcggccac
     2641 ctctggcagt tcgacgcgcg gagcggccgc ggcatcaacc cggccgcgtc ccggctgacg
     2701 cgccatgcgg tgaagatcga ggccggcggc gtgtgggtgc agaccgtccc cgaaccggac
     2761 tccggacaag gaggcgcatg acgaccgagc aggcaacgct ggtgggcccg gtgattcgcg
     2821 cgggcgaggt cgcggacgcg gtggcgctcg cgatcgagga cgacaacccc ggtcgcgccg
     2881 tccgcgtgac ggatcgcggc gactacgtcc gcatcgaggt cgacgggcgc tgccggctca
     2941 cccggcgctc gctcgagcac cacctcggcc gcgagctctc gctgccccag ctcgagatcg
     3001 agatgccgag cttcgcgggc cggctgtcga cccggaccga cgagtacgtc tggtacttcg
     3061 agaacgcagg aggcggccat gactgacgac gtggcggacg cgcgcgcccg gcggcgcgcc
     3121 cagcggctgc gaacgtggag ccggctcggc cacctcggcc gggtcccgac cgagtacgag
     3181 atcgtcacgc acggcatgaa ctacacgacg agctcccgga cgccgctcga ggcggggccg
     3241 gacacgttcg gcaatcgctg gatcgccgag caccgcgacg cggtggccgt gcgcgtcgac
     3301 gcatgggacg acttccgcga tcccgacgag ctcacctacc gcaagtacaa ccggtgccag
     3361 gacgccgcgg aggcgttcgt cgatcaggtg ctcgaggact atggcgagcg ccgcgacgcc
     3421 gaccgcggcc accacccgcg ctggctgtac gcgctcggca tgctgctcac gccgcagcgc
     3481 tatccggtgc acggcctgca gatggtcgcg gcctacgtcg cgcagatggc gccgtcgagc
     3541 tacatcacca acgccgcgac gttccaggcc gccgacgagc tgcgccgcgt gcagcgcatc
     3601 gcctaccgca cgcggcagct cgacctcgcg catccggcgc tcggcttcgg cgggaccgag
     3661 cgcgcgacct tcgagaccca caagcgctgg caggccgcgc gcgcggcgat cgagaagctg
     3721 ctggtcgtgt tcgactggga cgaggcgttc accgcgctcg agctcgcgat caagccggcg
     3781 tacgacgaga tctac
//
LOCUS       DASVOR010001857         9420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_281531,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001857 DASVOR010000000
VERSION     DASVOR010001857.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9420
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_281531"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VF516_34525"
     CDS             complement(<1..741)
                     /locus_tag="VF516_34525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112915.1"
                     /translation="MTRSSPARSRAMPWQVAAIAAGLLASATPAAVAAPPAAPAAAPA
                     APPGPRQTEQDEYTRYELLAPDTASFKIDYEVTATTPGARAFWNPIRKGSVASDEAVF
                     DLMTGAPLTFEQVSGAQARAHGLADADPDSDYIEVHLARRVPPDGGQARLRIVKTYKD
                     AKSYFQQGDTLVFDRSLGIRRNTVVLPAGYQLTDCNVPSQVLTEPDGRVRISFLHQAP
                     GPAALVVKARPGAPTGDAAKPRPLTTARS"
     gene            complement(766..2322)
                     /locus_tag="VF516_34530"
     CDS             complement(766..2322)
                     /locus_tag="VF516_34530"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEX8112916.1"
                     /translation="MESGARSGTPAHPDSSRGAVLAIAGATLVAHLAVSWRHGFFRDE
                     LYFIDCGRHPAFGYVDQPPLVPLVAAATQSFGHSLVALRAVAGLAHAGTVVVCAALAG
                     LIAGEVGLSGRSARRVAAMAVAVSPMFLGLTTTLNTTAFEPLMWTSLAYAVVRAIVRG
                     EPRFLVIAGAVAGLALEAKYALPLFLVPLIAGVALGPARRILVTRQMLLGTALCIAIA
                     LPSAIWQLAHGLPFLALLRAASDGKNTVIPPLAFLSNQLQVMNPVLAPMWIGGVAWCL
                     LGRDLARVRLLAIAFLAVLLATMLLHGKDYYAAPAYGIAFALGAVAIERFVRRAIVRA
                     IYVVLAVAVGLIAAPYSMPILDPPALVTYMEWMGAKPQAQETNQNDATIPQGQSDMLG
                     WPELEARVAEVWRSLPSGERTQAAIVTTNFGEAGAINFFGADDGLPRARSGHNQYGLW
                     GPGEQDSRIVIRVGGDLARYRQLCDDATVAATFGVPYAMPYERDRPIVLCRGMHGGLR
                     SRWPELVHID"
     gene            complement(2506..3405)
                     /locus_tag="VF516_34535"
     CDS             complement(2506..3405)
                     /locus_tag="VF516_34535"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEX8112917.1"
                     /translation="MRDLNDLQFFAAVVGHRGFSAAARVLGVPKSRVSRRVAVLEERL
                     GVRLLERSTRRLHVTEVGQQIYGHARAAIEEADAVEDVALRMRSEPRGLVRITCPHGL
                     QEIISHPLPAFLAANPSLRVHVIITNRRIDLIEDGVDIAIRIRERLDTDADIQLKKIG
                     TSKRILVAAPRLLAEASRPTKPADLLRFPLLHHQEQPGSSAWTLMNRASEHETIDFQP
                     RLATNDFGILVGAACAGAGIALLPRANCTAELAAGRLVHVLASWGAPDGILHLVFTSR
                     RGMLPGVRAVVEFAANALRAATN"
     gene            3809..4699
                     /locus_tag="VF516_34540"
     CDS             3809..4699
                     /locus_tag="VF516_34540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112918.1"
                     /translation="MSILQSGVGKYTHLCRGEVMRRILAHIHTCSERFAQEPMFQYLR
                     DEKIDPEQRLCFMPMMAHFVFSFMDVNKYILRKESVDTPYQRLINIHSYEDASHWPWW
                     VSDMKTAGLDKTCSFTDAMLFTWSAATRRSRMLTYDFIAIAAQATPVQLLAIVETVES
                     TGYKFLSTTAEVCRQLGASPYVYYGTKHSAVESGHHMGTEDAVSYLEAIELSDAELET
                     TRALVDRLYAAYTEFVDEMHDWVAGHTIEELRSAAFFRERVTGEPSRPLSVQELLDHR
                     TPVRVAPSDDDVADVDRPSP"
     gene            complement(4703..5689)
                     /locus_tag="VF516_34545"
     CDS             complement(4703..5689)
                     /locus_tag="VF516_34545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112919.1"
                     /translation="MPRRPVPAGPWGRTWLAAAAIAVVAVAAIELAMRARGYRPSVKD
                     DEYAWAWQRARVSDGSPRTVAILGTSRIHLAFSPTAFADRLPGWTAVQLAIDGTQPAG
                     ALVDLAGDPEFRGVAIVDTLESGIVAENRNSQRAYVAAYHRRWRAPGAMAERWLATRV
                     QSRLAILSDTLLRILASGAAPPYVATFPDRTQFADYSLTDVAERRKKLLDRLAPTPPT
                     PQEADAWLASVLEMEPYIAEIQARGGQVVYLRMPTCDERWATDQITEPRPMYWNRLAA
                     STRAIAIHFADFSSLRDFECPDTSHIASKDGPRFTRAVIDILTDRGVFPRGR"
     gene            complement(5706..7118)
                     /locus_tag="VF516_34550"
     CDS             complement(5706..7118)
                     /locus_tag="VF516_34550"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX8112920.1"
                     /translation="MQFNTYLFACFFALVLAVHHAPLPWTARKLALLGFSYAFYAAWD
                     PAFVVLLWGSTVVDWFVARAMMRTARPGLRRALLAASLVFNLGLLGYFKYAGFLQRTL
                     AAVLGAFGVSLAVAPVDIVLPIGISFYTFMTLSYTIDVYLGRSRPARSFLDYALYVTF
                     FPHLVSGPIVRATELVPQFETERRASRAQLGWGLALLAIGLFQKVVLADGLLAPVVDK
                     VFQPRLAQVSTGDAWAGAVAFAGQIFFDFAGYSSCAIGVALCLGFALPDNFRAPYGAV
                     GISDFWRRWHMSLSSWLRDYLYIPLGGNRRGALRTHVNLALTMLIGGLWHGASWTFVA
                     WGGLHGGYLIAERLVRAAAPASPVWSRRAVQLALALVTFLLVCFAWVFFRATTFDQAF
                     RILAAMLGRGRGAGHPAWWLAVLPVAALVAVHWRMRATSLEAVVERCPWPVRSALLAG
                     AVVSLFLFSGIDRAFIYFQF"
     gene            complement(7120..8559)
                     /locus_tag="VF516_34555"
     CDS             complement(7120..8559)
                     /locus_tag="VF516_34555"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8112921.1"
                     /translation="MTAGPATALRRDLGPWASASIVVGTIIGTGVFLKTAVMAQLGGS
                     PAWVLAAWGAAGLLSFTGAMTYAELGGMFPAAGGEYIYLRQGYSPFMGYLYAWNRFWI
                     ATPGSVAAYAVGSATFVSTAVPLDGFAIELAGARFTGVQVVGLGLIVAFTAINCLNVR
                     SGGRLQTALTVMKAVMILGLAAGALLTPHGRWGHLAQGGAFPGWTAFGQMVLAALWAY
                     DGWNNLPMAAGEIRDARRNLPRATVLGMIAVLATYALVNVGYFHALPLSEVAQSSSDA
                     YPDAPAVAARAASAFLGGTAQAVLAIAMTISAISAMNGSILTGARVPYAVARDRLAPA
                     ALGHLSAGAAVPAISVVVQGALSCAFALTGSVDEQTDAVVFASWLFYALNSGSVLRLR
                     RREPGRDRPFRVPGFPVVPVVFIALAVLLIANTIGTTPKPSALGLAVTALGGLVYVMF
                     YRGKFRPDDPAAASTTPPGEVPAARVVSE"
     gene            complement(8556..>9420)
                     /locus_tag="VF516_34560"
     CDS             complement(8556..>9420)
                     /locus_tag="VF516_34560"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8112922.1"
                     /translation="EIAALGARVLHTPDLAATIARLRGDPALYFTTRDELLAAAQRAL
                     DRAKAAVPRVFSVLPRTDCVLRETPAYEAPYSTIAYYRQPHYDGTKPGEYFVNTYRPE
                     TRPRYELEALTWHESIPGHHLQIALAQELGALPLFRKLEGSTAFIEGWALYTERLADE
                     IGLYSSDLDRLGKLSYDAWRASRLVVDTGIHALGWTRTQAEAFMRAHTALTEVNISNE
                     VDRYIGWPGQALAYKVGQLEILALRAEAEAALGARFDLPAFHTVVLGAGAVTLPVLRD
                     RVHAWITAAAR"
BASE COUNT         1537 a         3192 c         3488 g         1203 t
ORIGIN      
        1 gctgcgcgcg gtggtgagcg gtcgcggctt cgcggcgtcg ccggtcggtg cgccggggcg
       61 ggccttgacg acgagcgcgg cggggccggg agcctggtgc aggaagctga tgcgaacgcg
      121 gccgtcgggc tcggtgagca cctgggacgg cacgttgcag tcggtgagct ggtagccggc
      181 gggcaggacg acggtgttgc gccggatccc gagcgagcgg tcgaacacga gggtgtcgcc
      241 ctgctggaag tagctcttgg cgtccttgta ggtcttgacg atgcggaggc gggcctggcc
      301 gccgtcgggc ggcacccggc gcgcgaggtg gacctcgatg tagtcgctgt cggggtcggc
      361 gtcggcgagg ccgtgcgccc tggcctgcgc gccggagacc tgctcgaacg tgagcggcgc
      421 gccggtcatg aggtcgaaca ccgcctcgtc gctggcgacg ctgcccttgc ggatcgggtt
      481 ccagaacgcc cgggcgcccg gcgtggtcgc ggtgacctcg tagtcgatct tgaacgatgc
      541 ggtgtccggc gcgagcagct cgtagcgggt gtactcgtcc tgctcggtct gcctgggtcc
      601 cggcggagca gcgggcgcgg ccgcgggggc cgccggtgga gccgcgacgg ccgccggcgt
      661 ggccgaagcg aggagtccgg ccgcgatcgc ggcgacctgc catggcatcg cgcgcgatcg
      721 cgcgggggag ctgcgtgtca tgggccggca gtataggcac gtccgtcagt cgatgtgcac
      781 gagctcgggc cacctgctgc gcagacctcc gtgcatgccg cggcacagga cgatcgggcg
      841 atcgcgctcg tacggcatcg cgtacggcac gccgaacgtc gccgcgaccg tcgcgtcgtc
      901 gcagagctgc cggtagcgcg ccaggtcccc gccgacgcgg atcacgatgc ggctgtcctg
      961 ctcgcccggc ccccacagcc cgtactggtt gtggccgctc cgcgcgcgcg gcaggccatc
     1021 gtccgcgccg aagaagttga tcgcgccggc ctcgccgaag ttggtcgtca cgatcgccgc
     1081 ctgagttcgc tcgccggacg gcagcgatcg ccagacctcg gcgacgcggg cctcgagctc
     1141 cggccagccg agcatgtcgg actggccctg cgggatcgtc gcgtcgttct ggttggtctc
     1201 ctgcgcctgc ggcttcgctc ccatccattc catatatgtg acgagcgcgg gcggatcgag
     1261 gatcggcatc gagtacggcg ccgcgatcag ccccacggcg acggcgagca ccacgtagat
     1321 cgcgcgcacg atcgcccggc ggacgaagcg ctcgatggcc accgcgccga gcgcgaacgc
     1381 gatcccgtag gccggcgcgg cgtagtagtc cttgccgtgg agcagcatcg tggcgaggag
     1441 cacggcaagg aaggcgatgg cgagcagccg cacgcgcgcc agatcgcggc cgagcaggca
     1501 ccatgcgacg cccccgatcc acatcggcgc gagcaccggg ttcatgacct gcagctggtt
     1561 cgagaggaac gcaagcggcg ggatcacggt gttcttgccg tccgatgccg cgcgcaggag
     1621 cgcgaggaac ggcaggccgt gtgccagctg ccagatggcg ctcggcagcg cgatcgcgat
     1681 gcacagcgcg gtaccgagca gcatctgccg cgtgaccagg atgcggcgcg cggggccgag
     1741 ggcaaccccg gcgatcagcg gcaccaggaa cagcggcagc gcgtacttcg cttccagcgc
     1801 caggcccgcg acggcgccgg cgatcaccag gaaccgtggc tcgccgcgca cgatcgcgcg
     1861 cacgacggcg tacgccaggg acgtccacat cagcggctcg aacgccgtcg tgttgagggt
     1921 cgtcgtcaag cccaggaaca tcggcgacac ggccacggcc atggcggcca cccgccgcgc
     1981 ggaccgtccg gacagcccga cttcacctgc gatcaagccc gccagggcgg cgcacaccac
     2041 gacggtcccc gcatgggcga gcccggccac cgcgcgcagc gcgaccaggc tgtggccgaa
     2101 cgactgcgtc gccgccgcca cgagcggtac cagcggcggc tgatcgacgt agccgaacgc
     2161 cggatgccga ccgcagtcga tgaagtacag ctcgtcgcgg aagaacccat gccgccacga
     2221 caccgcgagg tgcgcgacca gcgtcgcacc ggcgatcgcg agcaccgcgc cgcgggacga
     2281 atccggatgt gccggcgtgc cgctgcgcgc gcctgattcc atcggcgaca acctagcatg
     2341 cggccgcggc cttgcgccga tcgacgcgtg atcggcacgc cgtgcgtgac ggcgagtagc
     2401 gcaggagcaa gtccgccacg ccgtcacacc gcggaatccg gacagctcga ccgacatgag
     2461 agtgcccacc ctcggacggt ccggaggtag gggggaacca gagcgtcagt tcgttgccgc
     2521 gcgcagagcg ttcgcggcga attcgaccac cgcacggacg ccgggcagca tgccgcgccg
     2581 tgaggtgaag acgaggtgga gaatcccgtc aggtgcgccc caggacgcga gcacgtgcac
     2641 gagccggccc gctgccagct cggccgtgca gttcgcgcgg ggcaacaagg cgatcccggc
     2701 gcccgcacac gctgcgccga ccaggatccc gaagtcattc gtcgctagcc tcggctggaa
     2761 atcgatcgtc tcgtgctcgc tggcgcgatt catgagcgtc caggcgctcg atccgggctg
     2821 ctcctgatgg tgaagcaggg gaaaccgtaa cagatcggcc ggcttcgtcg gccggctcgc
     2881 ctccgcgagc agccggggag cggccaccag gatccgcttg ctcgtcccga tcttcttgag
     2941 ctggatgtcg gcgtccgtgt cgagccgctc gcgaatgcgt attgcgatgt cgaccccgtc
     3001 ctcgatgaga tcgatgcggc ggttggtgat gatcacgtgc acgcggagcg atggatttgc
     3061 ggcgagaaag gccggcaacg ggtgggagat gatctcctgc aggccatgtg gacaggtgat
     3121 ccggacgagc ccgcgcggct ccgagcgcat gcgcagcgca acgtcctcga cggcatcggc
     3181 ttcttcgatc gcggcccgag catgcccgta gatctgctgg cccacctcgg tgacatggag
     3241 gcgccgtgtg gaacgctcga gcagacgaac gccgagccgc tcctcgagaa cagcgacccg
     3301 gcggctgacg cgagacttcg ggacgccgag cacccgcgcg gccgcggaga acccgcggtg
     3361 gcccacgacg gcagcgaaga actggaggtc gttgagatcg cgcatcgtcg ttcggctcgt
     3421 accacagggt acagcccagg ggctgcctcc gcgggcgcgt ctcgcggaaa ggacctgcgc
     3481 tccggccatg ggggtcgaag caccagcggg ttggcgagga ggatctggaa gagttgagcg
     3541 agtgaagccg gcatgatctg tctcgtttcg tctgctcgca ccccgatcta tgtcgcaagc
     3601 cgcggcatcc tcggaagacg gcgccggcgc gactcggcgt tctccaggta ggacaatcgc
     3661 cggggaccgg gacgcgcgca ggtagccacc gaggcagaga gacatgtcga gccgctcgcg
     3721 tgcatgtgat cttcaccact gggaccaaaa ccagagaact cgacgtctct ctcttgtttg
     3781 gagaaaccga tatcgcgaga tcacgtcaat gtcgatattg cagtctggag tagggaaata
     3841 cacccatcta tgccgaggag aagtcatgcg ccgtattctg gctcacatcc acacctgtag
     3901 cgagcgcttt gcacaagagc ccatgttcca gtacctccgg gacgaaaaga tcgaccccga
     3961 acagcgactc tgcttcatgc cgatgatggc acacttcgtc ttttcgttca tggacgtcaa
     4021 caagtacatc cttcgcaagg agtcggtcga cacgccgtat cagcgcctca tcaacatcca
     4081 cagctacgag gatgcatcgc actggccgtg gtgggtcagc gacatgaaga cggcgggcct
     4141 cgacaagacg tgtagcttca cggatgcgat gttgttcaca tggagtgcag cgacccgccg
     4201 gtcgcggatg ctcacgtacg acttcattgc gatcgcggcg caggcaacgc cggtccagct
     4261 gctcgcgatc gtggagacag tcgagtcgac cggctacaag ttcctgtcga cgaccgccga
     4321 ggtgtgccgc cagctcggag ccagcccgta tgtctactac ggcaccaagc actcggcggt
     4381 ggagtccggc catcacatgg gcaccgagga tgccgtcagc tacctggaag cgatcgagct
     4441 atccgatgcg gagctggaga ccacccgggc gctggtcgac aggctgtacg ccgcgtatac
     4501 cgaattcgtc gatgagatgc atgactgggt ggccggccac acgatcgagg agctgcggag
     4561 cgccgcgttc ttccgcgagc gagtcactgg agaaccgagc cggccactga gcgtgcagga
     4621 gctgctggat caccggactc ccgtgcgggt tgcgccgagc gacgatgacg tggcggacgt
     4681 cgaccgcccc agcccctgat cgtcaccggc cacgcgggaa cacgccgcgg tcggtgagga
     4741 tgtcgatcac ggcgcgggtg aaccgcgggc cgtccttgga cgcgatgtgc gaggtgtcgg
     4801 ggcactcgaa gtcgcgcagc gacgagaagt cggcgaagtg gatcgcgatc gcgcgcgtgc
     4861 tggcggcgag ccggttccag tacatcgggc gcggctcggt gatctgatcg gtcgcccagc
     4921 gctcgtcgca ggtcggcatc cgcaggtaga cgacctggcc gccgcgggcc tggatctcgg
     4981 cgatgtacgg ctccatctcc agcacgctgg cgagccaggc gtcggcttcc tggggggtcg
     5041 gcggggtcgg cgcgagccgg tcgagcagct tcttgcggcg ctcggcgacg tcggtgagcg
     5101 agtagtcggc gaactgggtg cgatcgggga acgtggcgac gtacggcggg gcggcgccgg
     5161 acgcgaggat ccgcaggagc gtgtcggaca ggatcgcgag ccggctctgc acgcgggtcg
     5221 cgagccagcg ctcggccatc gcgcccggcg cgcgccaccg gcggtgatag gcggcgacgt
     5281 acgcgcgctg gctgttgcgg ttctcggcga cgatgccgga ctcgagggtg tcgacgatcg
     5341 cgacgccgcg gaactcgggg tccccggcga gatcgaccag cgcgccggcc ggctgggtgc
     5401 catcgatcgc gagctgcacc gcggtccagc ccggcaggcg gtcggcgaac gccgtcggcg
     5461 agaacgccag gtggatccgc gaggtgccga ggatcgcgac ggtgcgcggc gagccgtcgc
     5521 tgacccgcgc gcgctgccag gcccacgcgt actcgtcatc cttgaccgac gggcggtagc
     5581 cccgagcgcg catcgcgagc tcgatggcgg cgacggcgac cacggcgatc gcggccgccg
     5641 cgagccacgt cctgccccat ggcccggcgg ggaccgggcg gcgcggcacg tcgtgtcgag
     5701 tggggtcaga actggaagta gatgaaggca cggtcgatcc ccgagaacag gaacagcgag
     5761 accacggcgc cggcgagcag cgccgagcgc acgggccacg ggcagcgctc gacgacggcc
     5821 tcgagcgagg tcgcgcgcat ccgccagtgg acggcgacga gggcggcgac ggggagcacg
     5881 gcgagccacc acgcggggtg accggcgccg cggccgcggc cgagcatcgc ggcgaggatg
     5941 cggaacgcct gatcgaaggt ggtcgcgcgg aagaacaccc aggcgaagca gaccaggagg
     6001 aaggtgacga gggcgagcgc gagctggacg gcgcggcggc tccacaccgg cgaggccggc
     6061 gcggcggcgc gcaccaggcg ctcggcgatc aggtagccgc cgtgcaggcc gccccacgcg
     6121 acgaacgtcc acgacgcgcc gtgccacagg ccgccgatca gcatggtcag cgcgaggttg
     6181 acgtgggtgc gcagcgcgcc gcggcggttg ccgccgagcg ggatgtagag gtagtcgcgc
     6241 agccagctcg acagcgacat gtgccagcgc cgccagaagt ccgagatccc gaccgcgccg
     6301 tacggcgcgc ggaagttgtc gggcagcgcg aagcccaggc acagcgcgac gccgatcgcg
     6361 cagctcgagt agccggcgaa gtcgaagaag atctggccgg cgaacgccac cgcgccggcc
     6421 cacgcatcgc cggtcgagac ctgcgcgagc cggggctgga acaccttgtc gaccaccggc
     6481 gcgagcaggc cgtcggcgag cacgaccttc tggaacaggc cgatcgcgag cagcgcgagg
     6541 ccccagccga gctgcgcgcg gctcgcgcgg cgctcggtct cgaactgcgg cacgagctcg
     6601 gtcgcgcgca cgatcggccc cgacaccagg tgggggaaga acgtcacgta cagcgcgtag
     6661 tcgaggaacg accgcgccgg gcggctgcgc ccgaggtaga cgtcgatcgt gtaggacagc
     6721 gtcatgaacg tgtagaacga gatgccgatc ggcagcacga tgtcgaccgg cgccacggcg
     6781 agcgagacgc cgaaggcccc gagcaccgcc gcgagcgtgc gctgcaggaa ccccgcgtac
     6841 ttgaagtagc cgagcaggcc gaggttgaac accaggctcg cggcgagcag cgccctgcgc
     6901 aggccgggcc gcgcggtgcg catcatcgcg cgcgcgacga accagtcgac gacggtcgag
     6961 ccccacagga ggaccacgaa cgcgggatcc cacgcggcgt agaacgcgta gctgaacccg
     7021 agcagcgcga gcttgcgcgc ggtccagggc agcggcgcgt ggtgcaccgc gagcaccagc
     7081 gcgaaaaagc aggcgaacag gtaggtgttg aactgcacgt cactccgaga ccacgcgcgc
     7141 ggccgggacc tcgcccggag gcgtggtcga cgctgccgcc ggatcgtccg gccggaactt
     7201 gccgcggtag aacatgacat acaccagccc gccgagcgcg gtcaccgcca ggccgagcgc
     7261 gctcggcttc ggggtggtcc cgatcgtgtt ggcgatcagc agcaccgcga gcgcgatgaa
     7321 cacgaccggc acgaccggga agcccggcac gcggaacggc cggtcgcggc cgggctcgcg
     7381 gcggcgcagc cggagcaccg agccggagtt gagcgcgtag aacagccacg acgcgaacac
     7441 cacggcgtcg gtctgctcgt cgacgctccc ggtcagcgcg aacgcgcacg acagcgcgcc
     7501 ctggaccacc accgagatcg ccgggaccgc ggcgcccgcc gacaggtgac cgagcgccgc
     7561 cggggcgagc cggtcccgcg ccaccgcgta cggcacgcgc gcgccggtca ggatcgagcc
     7621 gttcatcgcc gagatcgccg agatcgtcat cgcgatcgcg agcacggcct gcgcggtccc
     7681 gccgaggaac gcggacgcgg cgcgcgcggc caccgcgggc gcgtcggggt atgcgtccga
     7741 gctcgactgc gcgacctcgg acagcggcag cgcgtggaag tagccgacgt tgaccagcgc
     7801 gtaggtcgcg agcacggcga tcatgccgag caccgtcgcg cgcggcaggt tgcggcgggc
     7861 gtcgcggatc tcgccggcgg ccatcggcag gttgttccag ccgtcgtacg cccacagcgc
     7921 ggcgagcacc atctgaccga acgcggtcca gccggggaac gcgccgccct gcgcgaggtg
     7981 gccccagcgg ccgtgcgggg tgagcagcgc gccggcggcc agcccgagga tcatcaccgc
     8041 cttcatcacg gtcagcgcgg tctgcagccg gccgcccgag cgcacgttga ggcagttgat
     8101 cgcggtgaac gcgacgatca ggccgagccc gacgacctgg accccggtga accgggcgcc
     8161 ggcgagctcg atcgcgaagc cgtcgagcgg caccgcggtc gagacgaacg tcgcgctgcc
     8221 gaccgcgtac gccgcgaccg atcccggcgt cgcgatccag aaccggttcc acgcgtagag
     8281 gtagcccatg aacgggctgt agccctggcg caggtagatg tactcgccgc cggccgccgg
     8341 gaacatgccg ccgagctcgg cgtaggtcat cgcgccggtg aacgacagaa ggcccgccgc
     8401 gccccacgcc gcgagcaccc acgccggcga gccgccgagc tgcgccatca ccgcggtctt
     8461 gaggaacacg ccggtgccga tgatcgtccc gacgacgatc gacgccgagg cccacgggcc
     8521 gagatcgcgg cgcagcgccg tggcgggccc ggcggtcacc gcgccgccgc cgtgatccag
     8581 gcgtggacgc gatcgcgcag caccggcagg gtgaccgccc cggcgccgag cacgacggtg
     8641 tggaacgccg gcaggtcgaa ccgggcgccg agcgcggctt cggcctcggc gcgcagcgcg
     8701 aggatctcga gctggccgac cttgtaggcc agcgcctggc cgggccagcc gatgtagcgg
     8761 tcgacctcgt tggagatgtt gacctcggtc agcgcggtgt gcgcgcgcat gaacgcctcg
     8821 gcctgggtgc gggtccagcc cagcgcgtgg atcccggtgt cgaccaccag gcgcgacgcg
     8881 cgccaggcgt cgtacgacag cttgccgagc cggtcgaggt ccgaggagta cagcccgatc
     8941 tcgtcggcga gccgctcggt gtacagcgcc cagccctcga tgaacgcggt cgagccctcg
     9001 agcttgcgga acagcggcag cgcgccgagc tcctgggcca gcgcgatctg caggtggtgg
     9061 ccggggatcg actcgtgcca ggtcagcgcc tcgagctcgt agcgcggccg ggtctcgggc
     9121 cggtaggtgt tgacgaagta ctcgccgggc ttggtgccgt cgtagtgcgg ctggcggtaa
     9181 taggcgatcg tcgagtacgg cgcctcgtac gccggggtct cgcgcagcac gcagtcggtg
     9241 cgcggcagca ccgagaacac ccgcgggacg gcggccttgg cgcggtcgag cgcgcgctgg
     9301 gcggcggcga gcagctcgtc gcgcgtcgtg aagtacagcg cggggtcgcc gcgcagccgg
     9361 gcgatcgtcg cggcgagatc cggggtgtgc agcacgcggg cgccgagcgc cgcgatctcg
//
LOCUS       DASVOR010001858         4050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_457725,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001858 DASVOR010000000
VERSION     DASVOR010001858.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457725"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF516_34565"
     CDS             <1..474
                     /locus_tag="VF516_34565"
                     /inference="COORDINATES: protein motif:HMM:NF024983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8112923.1"
                     /translation="LGELAAARHELTAIRDKLHSAREDTRHKLAAVERQAREQRDLLA
                     ATGLAVLFVDRELRIQHHTPPLQQLIHVWPHDRGRPISDLASCLEYRELAGDLRQVLD
                     TLAPIECEVCDRTGRPFLVRLSPHRSGDEVIGAVATFIDIAHVRGAAVQRRAAGS"
     gene            580..1593
                     /locus_tag="VF516_34570"
     CDS             580..1593
                     /locus_tag="VF516_34570"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEX8112924.1"
                     /translation="MSEDAAERRIGWIGWGLIAACAALLAPLLPPIMLALWLGALARV
                     LHRPLTRALRGRVRLAAAITMIALIAVLVPFALAFLSLAADTYQLVTQVLQSPRGKQV
                     LEQLVAPSARAGPGSSGLRDLILNQQERAWAILRQVAGTATRVVVELVVIGAGTYAVL
                     VDGGRAYPWLERHAPIDPDLLRRLRDAFFETGRGLFIGLGGAGLLQAIIATIAYLVLD
                     VPRALELGVLTFCCSIIPGVGTALVWAPIAAGLGLTGRTGAAIALAVCGIGVIGTLDN
                     FVKPVLARRGRLDLPTYVVMVAMFAGVMVIGPWGLLVAPLAVRLVKAVLEGAAANPTA
                     NEP"
     gene            complement(1618..2604)
                     /locus_tag="VF516_34575"
     CDS             complement(1618..2604)
                     /locus_tag="VF516_34575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8112925.1"
                     /translation="MQPPLSVLDLSPVGAGVAPSQAIRESIELARTADALGYTRYWFA
                     EHHNMANIATSAPEILIAQAAAVTTRIRLGAGGIMVPNHAPLHVVEVFRTLEALHPGR
                     IDLGLGRAPGTDPITAAALQRADREVNHQLAELLAFERGEFPASHPFARIVPMPSDAP
                     IGEIWMLGSTLAGAAIAAQLGLPYAFAGHFAMRHAASAIAHYRARFEPSAARREPYAI
                     LAVTAICAETDDAANRLAAPVRVAVVKNRTGRRAPIPTIDEALAYELTPDERAIADEF
                     LTGAAIGSPATVADRLRALARDTGAHELMLSTLIPDLAARQRALRLIADAMA"
     gene            complement(2673..3209)
                     /locus_tag="VF516_34580"
     CDS             complement(2673..3209)
                     /locus_tag="VF516_34580"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEX8112926.1"
                     /translation="MTKTLLFSTLFVSLVAGSALADDAKKPAPAKPVTVKLSDAKGKS
                     IGTAKLAPDAGGVKITLAVKGLPPGDHAIHIHETAKCDGPDFKSAGGHFNPEKKQHGT
                     DNPQGPHAGDMPNFTVDAKGGSTASVVAPGVTMDDGPHSVFTGGGTALVIHEKADDMK
                     TDPAGAAGNRIACGLIKK"
     gene            complement(3253..>4050)
                     /locus_tag="VF516_34585"
     CDS             complement(3253..>4050)
                     /locus_tag="VF516_34585"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEX8112927.1"
                     /translation="FNIWNRQGPWDRRLPLIRDGLVALDADVIGLQEVLAFGSLPSQA
                     HEIASGLGWHVYHAPAWQVGGGLTFGNAILSPHRLLDTQVLTLPTPPQLDSRAVVFAR
                     VDAPHGPIPVFVTHLTVQFHLCHVRRAQVVALSDHVARLAPIGEPPPVVLGDFNASPD
                     SDEIRFLRGLTSLDGRSVYFADCWATAATGPGYTYDRRNPYALRAHEPSNRIDYVFVR
                     GPDAHLRGEPIAARLALDQPTGGVWPSDHFAVVADIHAAPRGHDDEG"
BASE COUNT          498 a         1405 c         1564 g          583 t
ORIGIN      
        1 ctcggcgagc tcgcggcggc gcggcacgag ctgaccgcga tccgcgacaa gctgcacagc
       61 gcgcgcgagg acacccggca caagctcgcc gccgtggagc gccaggcgcg cgagcaacgc
      121 gatctgctcg ccgcgaccgg cctcgcggtg ctgttcgtcg atcgcgagct gcggatccag
      181 caccacacgc cgccgctcca gcagctcatc cacgtctggc cccacgatcg cgggcggccg
      241 atcagcgatc tggcgagctg cctcgagtac cgcgagctcg ccggcgacct ccgccaggtg
      301 ctcgataccc tcgcgccgat cgagtgcgag gtctgcgatc gcaccggacg gccgttcctc
      361 gtccggctct cgccccatcg cagcggcgac gaggtgatcg gtgcggtggc gaccttcatc
      421 gacatcgccc acgtgcgcgg cgccgccgtg cagcgccggg ccgcgggaag ctgaccggct
      481 cgctcgcctc gcgcttcctc gtagtccggc gtccctccag gaaccgctca tccggagacc
      541 gcgtcgcggc atggtccggt tcgtgcgtcg aacggcagaa tgagcgagga tgccgccgag
      601 cgcaggatcg gctggatcgg ctgggggctg atcgccgcct gcgccgcgct gctcgcgccg
      661 ctcctgccgc cgatcatgct ggcgctgtgg ctcggcgcgc tcgcgcgggt gctgcatcga
      721 ccgctgaccc gcgcgctccg cggtcgcgtg cggctggcag ccgcgatcac gatgatcgcg
      781 ctgatcgccg tgctcgttcc attcgcgctg gcgttcctct cgctcgcggc cgacacctac
      841 cagctcgtca cgcaggtgct gcagtcgccg cgcggcaagc aggtcctcga gcagctcgtg
      901 gcgccgagtg cgcgcgcggg gccgggcagc tcgggcctgc gcgacctgat cctcaaccag
      961 caggagcgcg cctgggccat cctccggcag gtcgccggga cggcgacgcg cgtcgtcgtc
     1021 gagctcgtgg tgatcggcgc cggcacctac gcggtgctcg tcgacggcgg ccgggcgtac
     1081 ccgtggctgg agcgccacgc tccgatcgac ccggacctgc tccgccggct gcgcgacgcg
     1141 ttcttcgaga ccgggcgcgg gctgttcatc ggcctcggcg gcgcggggct gctgcaggcg
     1201 atcatcgcga cgatcgcgta cctcgtgctc gacgtccccc gcgcgctgga gctcggcgtg
     1261 ctcacgttct gctgctcgat cattccaggc gtcgggaccg cgctcgtgtg ggcgccgatc
     1321 gccgccgggc tgggcttgac cggtcgcacc ggagcggcga tcgcgctggc cgtgtgcggg
     1381 atcggcgtga tcgggaccct cgacaacttc gtgaagccgg tgctcgcccg gcgcgggcgg
     1441 ctcgatctcc cgacgtacgt cgtgatggtg gcgatgttcg ccggggtcat ggtgatcggc
     1501 ccatggggcc tgctggtcgc cccgctcgcg gtgcgcctcg tcaaggccgt gctcgagggc
     1561 gcggcggcga acccgaccgc gaacgaaccg tgagccgcga cgcgatcatg ccatcgctca
     1621 ggccatcgcg tcggcgatca gccggagggc gcgctggcgg gcggcgagat cggggatcag
     1681 ggtcgacagc atcagctcgt gggcgccggt gtcgcgggcc agcgcgcgca gccggtcggc
     1741 gacggtggcc gggctgccga tcgccgcgcc ggtgaggaac tcgtccgcga tcgcgcgctc
     1801 gtcgggcgtg agctcgtagg ccagggcctc gtcgatcgtc gggatcgggg cccggcgccc
     1861 ggtgcggttc ttgaccacgg cgacccggac cggcgcggcc aggcggttcg cggcgtcgtc
     1921 ggtctcggcg cagatcgcgg tcaccgcgag gatcgcgtac ggctcgcgcc gcgcggccga
     1981 cggctcgaac cgcgcgcggt agtgggcgat cgcagaggcc gcgtggcgca tcgcgaagtg
     2041 gccggcgaac gcgtacggca ggccgagctg ggcggcgatc gcggcgccgg ccagggtcga
     2101 gccgagcatc cagatctcgc cgatcggcgc gtcggacggc atcggcacga tccgggcgaa
     2161 cggatgcgac gccgggaact cgccgcgctc gaacgcgagc agctcggcga gctggtggtt
     2221 gacctcgcgg tccgcgcgct ggagcgccgc ggcggtgatc ggatcggtcc cgggcgcgcg
     2281 accgaggccg agatcgatcc ggcccgggtg cagcgcctcg agcgtgcgga acacctccac
     2341 gacgtggagc ggcgcgtggt tggggaccat gatgccgccg gcgccgagcc ggatccgggt
     2401 ggtgaccgcc gcggcctgcg cgatcaggat ctcgggcgcc gaggtcgcga tgttggccat
     2461 gttgtggtgc tcggcgaacc agtaccgggt gtagccgagc gcgtcggcgg tgcgcgcgag
     2521 ctcgatcgac tcgcggatcg cctggctcgg cgcgacgccg gcgccgaccg gcgagagatc
     2581 gaggaccgag agcggcggct gcatggcgac agcttggccc gggccggccg ggccgtcgag
     2641 cgcggcgacg gcgcgcccgg cgacctcgcg ggttacttct tgatcaggcc acaggcgatg
     2701 cggttgccgg cggcgcccgc cggatcggtc ttcatgtcgt cggccttctc gtggatcacc
     2761 agcgcggtgc cgccgccggt gaacaccgag tgcgggccgt cgtccatcgt cacgcccggc
     2821 gcgacgacgc tggcggtcga gccgcccttg gcgtcgaccg tgaagttcgg catgtcgccg
     2881 gcgtgcgggc cctgcgggtt gtcggtgccg tgctgcttct tctcggggtt gaagtggccg
     2941 ccggccgact tgaagtccgg gccgtcgcac ttggcggtct cgtggatgtg gatcgcgtgg
     3001 tcgcccggcg gcaggccctt gaccgcgagc gtgatcttca cgccgcccgc atccggggcg
     3061 agcttggcgg tgccgatgct cttgcccttg gcgtcgctga gcttgacggt gaccggcttg
     3121 gcgggagccg gcttcttggc gtcgtcggcg agcgcggagc cggcgaccag ggagacgaac
     3181 agggtggaga acagaagcgt cttggtcatg gtggagatat tcggacgtcc ggtgcgcggc
     3241 gtccagtcct cgtcagcctt cgtcgtcgtg accgcgcggc gcggcgtgga tgtccgcgac
     3301 gaccgcgaag tgatcggacg gccacacgcc gccggtcggc tgatcgagcg ccagccgcgc
     3361 cgcgatcggc tcgccgcgca ggtgggcgtc ggggccgcgc acgaacacgt agtcgatccg
     3421 gttcgacggc tcgtgcgcgc gcagcgcgta ggggttgcgg cgatcgtagg tgtagcccgg
     3481 tccggtcgcg gccgtggccc agcagtcggc gaagtacacc gagcggccgt cgagcgaggt
     3541 cagcccgcgc aggaagcgga tctcgtcgga gtcgggcgac gcgttgaagt cgccgagcac
     3601 gaccggcggc ggctcgccga tcggcgcgag ccgggcgacg tgatcgctca gcgcgacgac
     3661 ctgggcgcgc cggacgtggc agaggtggaa ctgcacggtc aggtgggtga cgaacaccgg
     3721 gatcgggccg tgcggcgcgt cgacccgggc gaacaccacc gcgcggctgt cgagctgcgg
     3781 cggggtcggc agcgtcagga cctgggtgtc gagcagccgg tgcggcgaca ggatcgcgtt
     3841 gccgaacgtc aggccgccgc cgacctgcca cgccggcgcg tggtagacgt gccagcccag
     3901 cccggaggcg atctcgtggg cctggctcgg cagcgagccg aacgcgagca cctcctggag
     3961 cccgatgacg tcggcgtcga gcgcgaccag gccgtcccgg atcaggggca gccgccgatc
     4021 ccatggaccc tggcggttcc agatgttgaa
//
LOCUS       DASVOR010001859         2190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_427930,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001859 DASVOR010000000
VERSION     DASVOR010001859.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427930"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1722
                     /locus_tag="VF516_34590"
     CDS             <1..1722
                     /locus_tag="VF516_34590"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8112928.1"
                     /translation="LVLGITGDVPESWGVLDHWKYELSYNYGIGEAVAGTTGQLNKLH
                     AADALGPSMRDADGTPICVRVPGDPTTKIIYELHAADGLGASGRIPLQPVPCVPLDPF
                     LPAGRIPQDQLKSLTFSDVGAGTDTLHTYLASANGRVAELPDHGEISLSLGGDAHYEV
                     GNLEAPHVASTGYTTDNQVLDTHGKFHLFEGFGELAIVPITGGDLAQRIEITLGAHAV
                     RNNRFGSSLTYKAGGLVRTAGGIAVRGTYATAFRSPSVFDLFGGHTETLPAVEDPCDA
                     RPPGAGDATRTLDPRVQAQCTAQGIPVGTRFTTSQQPAVIGGNSDLQPETAATATVGV
                     VIEPPQLPGVTLTADYWHIAIDDAIEALGVPTIFASCYARGIQAFCDQIHRDFTTHRI
                     LHVDQFLQNVPRTVTSGIDVALWHDVRLAQLGRIHTALEAQYLLRYDLHAADQVIHGV
                     GYYDLGVYPHYKANLSSRWTHPGGASGGFTLRYVGSYQECAGDDCNTPRNLAMSRDVD
                     RYFKLDLFGGYDLPSWLGKTTLQAGISNVFDAAPPVVYNAPAANSDATTYDFLGRMVY
                     LRMSQLF"
     gene            1876..>2190
                     /locus_tag="VF516_34595"
     CDS             1876..>2190
                     /locus_tag="VF516_34595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112929.1"
                     /translation="MTLLAACRAAPIGNHGPDAPDAPDAPAAPCSSHRVGQVTVIGAP
                     ASAVPQLAVLEGTLDDPERTERVARVAADGLRALGYADVSIAVARRPGCRINLAVTVA
                     LGP"
BASE COUNT          319 a          860 c          691 g          320 t
ORIGIN      
        1 ctcgtcctgg gcatcaccgg cgacgtcccc gagtcgtggg gcgtgctcga ccactggaag
       61 tacgagctgt cctacaacta cgggatcggc gaggcggtcg ccgggacgac cggccagctc
      121 aacaagctgc atgccgccga cgcgctcggc ccgagcatgc gcgacgccga cggcaccccg
      181 atctgcgtcc gcgtgccggg cgatcccacg accaagatca tctacgagct gcacgcggcc
      241 gacggtctcg gcgccagcgg aaggatcccg ctccagccgg tcccgtgcgt cccgctcgat
      301 ccgttcttgc ccgccggcag gatcccgcag gatcagctca agagcctgac gttcagcgat
      361 gtgggcgccg ggaccgacac cttgcacacc tacctcgcca gcgcgaacgg ccgcgtcgcc
      421 gagctccccg accacggcga gatctcgctg tcgctcggcg gcgatgctca ctacgaggtc
      481 ggaaacctgg aggccccgca cgtcgcgagc accggctaca ccaccgacaa ccaggtcctg
      541 gatacccacg gcaagttcca cctgttcgag ggcttcggcg agctggcgat cgtcccgatc
      601 accggcggcg atctcgccca gcggatcgag atcaccctcg gcgcccatgc ggttcgcaac
      661 aatcgcttcg gctccagcct cacgtacaag gccggcggcc tggtccgcac cgccggcggc
      721 atcgccgtgc gcggcaccta cgccaccgcg ttccgctccc cgtcggtgtt cgacctgttc
      781 ggcggccaca ccgagaccct gcccgccgtc gaggatccgt gtgacgcccg gccacccggc
      841 gccggcgacg ccaccaggac gctggatccg agggtccagg cccagtgcac cgcgcagggc
      901 atcccggtcg gcacccggtt caccaccagc cagcagccgg cggtgatcgg cggcaactcc
      961 gatcttcagc ccgagaccgc cgccaccgcc acggtcggtg tggtgatcga gccgccccag
     1021 ctcccgggcg tcaccctcac cgccgactac tggcacatcg ccatcgacga cgcgatcgag
     1081 gcgctcggcg tcccgacgat cttcgccagc tgctacgccc gcggcatcca ggcgttctgc
     1141 gatcagatcc accgcgactt caccacccac cggatcctcc acgtcgacca gttcctgcag
     1201 aacgtccccc gcaccgtgac gtctggcatc gatgtcgcgc tgtggcacga cgtccggctc
     1261 gcccagctcg gccggatcca caccgcgctc gaggcccagt acctgttgcg ctacgacctc
     1321 cacgccgctg atcaggtgat ccacggcgtc gggtactacg acctcggcgt ctacccgcac
     1381 tacaaggcca acctgtcgag ccgctggacc caccccggcg gcgcgtccgg cggcttcacc
     1441 ctgcgctacg tcggctcgta ccaggaatgc gccggcgacg actgcaacac tccccgcaac
     1501 ctcgccatgt cgcgcgacgt cgatcgctat ttcaagctcg acctgttcgg cggctacgac
     1561 ctgccgtcct ggctcggcaa gaccaccctg caggccggca tcagcaacgt gttcgacgcc
     1621 gccccgccgg tggtctacaa cgcccccgcc gccaactccg acgccaccac ctacgacttc
     1681 ctcggccgca tggtctacct ccgcatgtcc cagctgttct agcgatctcg aggcgggtag
     1741 gtcaccgtca cccgcggggt gcatcgcgtc tatcgcggaa tgcgctgccg ccgagatctc
     1801 gcgaggcgat tcgcgggctt gcgcgctgga gcgcggcttg ctggagcgcc gctcgtgcct
     1861 cggcggtggc tgatcgtgac cctcctggca gcgtgccgcg cggcgccgat cgggaaccat
     1921 ggtccggatg ctccggatgc tccggatgct ccggccgcgc cgtgctcgtc gcaccgggtc
     1981 ggccaggtca cggtgatcgg cgcgccggcc agcgcggtgc cgcagctcgc ggtgctggag
     2041 ggcacgctcg acgaccccga gcgcaccgag cgggtcgcgc gggtcgccgc cgacgggctg
     2101 cgcgcgctgg gctacgccga cgtgtcgatc gcggtggcgc ggcggccggg ctgccggatc
     2161 aacctcgcgg tcaccgtcgc gctcggcccg
//
LOCUS       DASVOR010001860         2405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_487241,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001860 DASVOR010000000
VERSION     DASVOR010001860.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487241"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VF516_34600"
     CDS             complement(<1..751)
                     /locus_tag="VF516_34600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112930.1"
                     /translation="MASGSAGAGHPAPPSSVALDLVPIPIAIGAVVLAILAIAGLREA
                     RERSRFVPGPWRYVVAVPPAARPMLQRRAIEDAATDLTWRAPGAGRELDVARSIAATM
                     ERGGLPTLVYCRQAAAPRYLVLEDTAGGAGRWQPIYDELLSGLAREGVEIERFRFVAS
                     PEHCVRPDGQAVRFGELIDHADALIVIGDGDAAIDPLDGRRAGWIASLRQIPRRLWIN
                     PVPPVRWSPGARAIAEDTPMEHGVARAVATLH"
     gene            complement(1582..>2405)
                     /locus_tag="VF516_34605"
     CDS             complement(1582..>2405)
                     /locus_tag="VF516_34605"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8112931.1"
                     /translation="RTPTSPEPDPKMTPPVDIIRPSTEPLARPAALTGEPGCGKTALA
                     WAVAAQLGCPVIEFHTKSTSTARDLLYQVDALRRFHDASTGDARARDAGHYLAYRALG
                     EAIRSPTTTVVLIDEIDKAPRDFPNDLLNELDRMEFDVPEVEPPLRFRATARHFVLIT
                     SNSERRLPMPFLRRCVYAHVEFPDAETLAQIVELHCGAPARSFVALAVRRFLELRATP
                     GLVKTPATGELIAWVRVLQRIGVDERALDRAPPGELPALETLIKMIDDLRTVRAS"
BASE COUNT          323 a          880 c          879 g          323 t
ORIGIN      
        1 ggtgcagcgt cgcgaccgcg cgggccacgc cgtgctccat cggcgtgtcc tcggcgatcg
       61 cgcgggcccc cgggctccag cgcaccggcg ggacagggtt gatccacagc cgccgcggga
      121 tctggcgcag cgaggcgatc cacccggcgc ggcggccatc gagcggatcg atcgcggcgt
      181 cgccatcgcc gatcacgatc agcgcgtcgg cgtgatcgat cagctcaccg aaccggacgg
      241 cctgaccgtc gggccggacg cagtgctcgg ggctggcgac gaagcggaag cgctcgatct
      301 ccacgccctc gcgcgccagg ccggacagga gctcgtcgta gatcggctgc cagcggccgg
      361 ccccgcccgc ggtgtcctcc agcacgaggt agcgcggcgc ggcggcctgc cggcagtaca
      421 ccagggtcgg gaggccgccg cgctccatgg tggccgcgat cgaccgcgcg acgtcgagct
      481 cccggcccgc gccgggtgcc cgccaggtca gatcggtggc ggcgtcctcg atcgcacgcc
      541 gctgcagcat cggcctggcc gccggcggca ccgcgaccac atagcgccac ggcccgggga
      601 cgaaccggct ccgctcgcgc gcttcgcgta ggcccgcgat cgcgaggatc gcgagcacga
      661 ccgcgccgat cgcgatgggg atgggcacga ggtcgagcgc gacgctggat ggcggtgccg
      721 ggtggccggc gcctgcggag ccactcgcca ttcctctagg ctgctggccg gcgcctgcgg
      781 agccgcttgc cgttcctcca ggctgctggc ctgtgcccgc agcgttgctc acctgtccgg
      841 caggttgcgg gccagggccg cttgtcgagc cagcatgctg cgggttgggg ccactgccct
      901 cgccgccaag ctgctggcca gccccactgc ctttgccggt gagctgttgg ctagagttac
      961 cgctgatgcc agtagactga tggcgaaagc taccgccgat gccggcaggc tgctggctgg
     1021 acctgctacc tgtgctgcca agctgctggc tgggtgcgct gtctctaccg gcaggctgct
     1081 ggctggaccc gctgcctgta ccggccagct gctggctgga tccgttgcac gccagcgact
     1141 caccagggcc cgacgcaggg tcgcccgcgc cgagccatag cgccagcacc aggccgaccg
     1201 cgatggtgac cgccgcgatc gcgcgcagcc gcaaccggcg gaccgcccgc ggctgctccg
     1261 agctcggccg tgatggctcc gcctccacgg ccggcgaccg ctcgccatgg gccgcaggca
     1321 ccgcacgttc cctgcgagcc accggcgcca ccgtgcgcgc cgacgcgccg ctcacccatc
     1381 gcgcccagca cgcgtcgaac gcatcgcgct cacccgggtc ggtcaccacc agcgccgcta
     1441 ccgccgtacg cagcagctcg ggagtccacg ccgcttcaca ggcgagcagc cggccgatcc
     1501 ggacgtgatc gtcgatcccc atcgcgaagc cctcggcgcg gagcaggtcg tacagctcgg
     1561 cgagcggcgg cgcccgcaag gtcagctcgc ccggaccgtg cgcaggtcgt cgatcatctt
     1621 gatcagcgtc tccaacgcgg ggagctcgcc cggcggcgca cgatcgagcg cgcgctcgtc
     1681 gacgccgatc ctttggagca cccgtaccca cgcgatgagc tcgccggtcg ccggcgtctt
     1741 gaccagcccc ggcgtcgcgc gcagctccag gaaccggcga acggcgagcg cgacgaacga
     1801 tcgcgcgggc gcgccgcagt gcagctccac gatctgcgcc agggtctcgg cgtcggggaa
     1861 ctcgacgtgg gcatagacgc agcggcgcag gaacggcatc ggcagccgcc gctcgctgtt
     1921 cgacgtgatc agcacgaagt gccgcgcggt ggcgcggaac cggagcggcg gctcgacctc
     1981 cggcacgtcg aactccatcc ggtcgagctc gttcaggagg tcgttgggga agtcgcgcgg
     2041 ggccttgtcg atctcatcga tcagcaccac cgtcgtggtc ggcgagcgga tcgcctcgcc
     2101 gagcgcgcgg taggcgaggt agtggccggc gtcgcgcgcg cgagcgtcgc cggtggatgc
     2161 atcgtggaac cggcgcagcg catcgacctg gtagagcaga tcgcgcgccg tcgacgtcga
     2221 cttggtgtgg aactcgatga ccgggcagcc cagctgggcg gcgaccgccc acgcgagcgc
     2281 agtcttgccg cagcccggct cgccggtgag cgcggccggc cgggcgagcg gttcggtcga
     2341 gggacggatg atgtcgaccg gtggggtcat cttggggtcc ggctccgggg acgttggagt
     2401 gcggt
//
LOCUS       DASVOR010001861         4478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340525,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001861 DASVOR010000000
VERSION     DASVOR010001861.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340525"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1779)
                     /locus_tag="VF516_34610"
     CDS             complement(121..1779)
                     /locus_tag="VF516_34610"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEX8112932.1"
                     /translation="MATRSLQAILVLAAAHAAVLAAPAGVRVVDGRYKLSTVVKDTAF
                     AGVNGATIGGDGALYVVHTGDGTTTRIDLKTLAATEFVHSYQGVWISDDITNDGKGTF
                     YATGTTPLVGEVYRIDAAGNKTVIARGLTAPNGIQFNRKTGRLFVTECFQANRVFELD
                     PAGGKEPRLIVKADTIPVPEGFDFDPDTNDLIIPDMGTGRILRVHPDTGAIQPIADKF
                     TAPIALKVGPDKKAYFPELGGAVYRLSLDGKTRDKLAQLPPGLDNLAITPDGRLFITS
                     YWDATVYEVATDGSGKFKTLFPRGPNTLTGVVVKNGQVLVSDAIMIRTVQKGEYAPTR
                     LNAWASHGMPLTLGIIDGPGDQVLWPDAVNNAIAIGNPKDGTFKPVAGGLNRPVAVVM
                     DAGKTRLYVAEYGAGQVTEISLADGAKRPLVTGLDGPIALAVVGDALYIAEARTARIR
                     RAPLAGGKSEVLLSAEVGKVGALAADGKGGLIAVDVIGRRVLHIDLRKLAITAIADGL
                     PVGYGVVGSGPSAVEFPTPLHVTADGDIYLGTAGRGVIELKAGK"
     gene            1895..3229
                     /locus_tag="VF516_34615"
     CDS             1895..3229
                     /locus_tag="VF516_34615"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8112933.1"
                     /translation="MRHLQNDRVVDMIRDRRAILALLTGLNFLNFIDRAVMAAVLPEV
                     TKGKGLGLSDFEGGLLNTAFLIGLFATSPLFGARADKGARKGLITLGVIIWSLATVAS
                     GLASGFWTLFAARIVVGVGEASYAVLAPTIIDDMFPPERKGSALSTFYLAIPLGYAMG
                     YAFGGQLAKHWDWRTAFFVVGGPGIVLALSVLLIAEPKRQLLGVKAKLIEGLRELAVL
                     PQFRRTVLGYTAYTAALAGFSIWAPKFLLRAFPSELDIGSANLYLGAVLMVAGTIGIW
                     IGGRYANRALVHHRVAPDEPYDSGRNKRAVNSLLRIAAVAMALSAPLSALGFLVPGAV
                     AYFVISFVVEICLFATTSPVNAALLRAVPAERRASAMAATVFSIHLFGDLWSAAALGL
                     LLDNLPLKIAMMSLPLTFAWSAYIWRPRQREAEAPSATGPDPVPEARVHTGT"
     gene            3263..4444
                     /gene="sucC"
                     /locus_tag="VF516_34620"
     CDS             3263..4444
                     /gene="sucC"
                     /locus_tag="VF516_34620"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEX8112934.1"
                     /translation="MKIHEYQGKQLFRKYGVPVPIGIPAMTVEEALAAIPKVQADAGT
                     DVVVVKAQIHAGGRGKGGGVKVAKTRTDAEAAVKKIFGMQLVTPQTGPEGKKVQRLLV
                     EQGLAIEHEYYLGMLLDRATGRVTVMASTEGGMDIEEVAAKHPEKILKHAIDPATGWQ
                     DWQSRQLAHELGMTGKAGEALAGFLRPLTRCYDDMDCSLLEINPLITTTDGRVLALDA
                     KINFDDNALYRHPELENWRDPSEEDAAEAEAKKWDLSYISLDGNIGCMVNGAGLAMST
                     MDIIKFYGGAPANFLDVGGGATAEKVTAAFKIITRDPDVKAIFVNIFGGIMKCDTIAQ
                     GVIAAVKEVGLQVPLVVRLEGTNVELGKKMLAESKLNIVAAADMADGAQKVVKAAKEA
                     R"
BASE COUNT          661 a         1512 c         1598 g          707 t
ORIGIN      
        1 cgccgccccg cggcctcacc gcatcccggc gcgcatcctg gagcttcgcg atgtcccacc
       61 gccgtgaccg cccgcgctcc cggccctcgc cgcgccaggg tgcacccggg cggccgccgt
      121 ctacttccct gccttgagct cgatcacgcc gcggccggcg gtgccgaggt agatgtcgcc
      181 gtcggcggtg acgtggagcg gggtcgggaa ctcgaccgcg gacgggccgg agccgaccac
      241 gccgtagccc accggcaggc catcggcgat cgcggtgatc gccagcttcc ggaggtcgat
      301 gtgcagcacg cgccggccga tcacgtcgac cgcgatcagg ccgcccttgc cgtcggcggc
      361 cagcgctccg accttgccga cctcggcgga cagcaacacc tcggacttgc cgccggcgag
      421 cggcgcccgg cggatccgcg cggtgcgggc ctcggcgatg tagagcgcgt cgccgaccac
      481 ggcgagcgcg atcgggccgt cgagcccggt gaccagcggc cgcttggcgc cgtcggcgag
      541 gctgatctcg gtgacctggc cggcgccgta ctcggcgacg tagagccggg tcttgccggc
      601 atccatcacc accgcgaccg ggcggttgag gccgccggcg accggcttga acgtgccgtc
      661 cttcgggttg ccgatggcga tcgcgttgtt gaccgcgtcg ggccacagca cctgatcgcc
      721 ggggccgtcg atgatgccga gcgtgagcgg catgccgtgc gaggcccacg cgttgagccg
      781 ggtcggcgcg tactcgccct tctgcacggt gcggatcatg atcgcgtcgc tgaccaggac
      841 ctggccgttc ttgaccacca cgccggtcag cgtgttcggg cccctgggga acagggtctt
      901 gaacttgccg ctgccgtcgg tggcgacctc gtagaccgtg gcgtcccagt agctggtgat
      961 gaacagccgg ccgtcggggg tgatcgcgag gttgtcgagg ccgggcggca gctgggcgag
     1021 cttgtcgcgg gtcttgccgt cgaggctcag ccggtagacc gcgccgccga gctcggggaa
     1081 gtaggccttc ttgtcgggcc cgaccttgag cgcgatcggc gcggtgaact tgtcggcgat
     1141 cggctggatc gcgccggtgt cggggtggac ccggaggatg cggccggtgc ccatgtcggg
     1201 gatgatcagg tcgttggtgt cgggatcgaa gtcgaagccc tcggggaccg ggatcgtgtc
     1261 ggccttgacg atcagccggg gctccttgcc gccggccggg tcgagctcga acacccggtt
     1321 ggcctggaag cactcggtga cgaacagccg gccggtcttc cggttgaact ggatgccgtt
     1381 gggggcggtc agaccacgcg cgatcacggt cttgttcccg gcggcgtcga tccggtagac
     1441 ctcgccgacc agcggcgtcg tgccggtcgc gtagaacgtg cccttgccgt cgttggtgat
     1501 gtcgtccgag atccacacgc cctggtagga gtggacgaac tcggtcgccg ccagggtctt
     1561 gaggtcgatc cgggtcgtgg tgccgtcgcc ggtgtggacg acgtagagcg cgccgtcgcc
     1621 gccgatcgtc gcgccgttga cccccgcgaa cgcggtgtcc ttgacgacgg tcgacagctt
     1681 gtaccggccg tcgacgacgc gaacgccggc gggcgcggca agcacggccg cgtgggctgc
     1741 ggccagcacc agaatcgcct gcagcgagcg cgtggccatg gctgcgatcc gcattgcgtg
     1801 actcctggga aaattcgccg atgtggctgt gtggggtcgg aggtactaga catcgccctg
     1861 gtaagattcg tcaagaccgg cccgccggag gtgcgtgcga cacctccaga atgatagggt
     1921 ggtggacatg atccgtgatc gccgagcaat cctggcgttg ctcacggggc tcaacttcct
     1981 caacttcatc gatcgcgcgg tgatggcggc ggtgttgccc gaggtcacca agggcaaggg
     2041 cctcggcctg tccgacttcg agggcggcct gctcaacacc gcgttcctga tcggactctt
     2101 cgcaaccagt ccgctgttcg gcgcccgcgc cgacaagggc gcgcgcaagg gactcatcac
     2161 gctcggcgtg atcatctgga gcctggccac ggtggccagc ggactggcga gcgggttctg
     2221 gaccctgttc gcggcgcgga tcgtggtcgg cgtcggcgag gcgagctatg cggtgctcgc
     2281 gccgacgatc atcgatgata tgtttccgcc cgagcgcaag ggctcggcgc tgtcgacgtt
     2341 ctacctcgcg atcccgctcg gctacgcgat gggctacgcc ttcggcggcc agctggccaa
     2401 gcactgggac tggcgcacgg cgttcttcgt cgtcggtggg ccggggatcg tgctcgcgct
     2461 gagcgtgctg ctgatcgccg agccgaagcg ccagctgctc ggtgtcaagg ccaagctcat
     2521 cgaggggctg cgcgagctcg ccgtgctgcc gcagttccgc cgcaccgtgc tgggctacac
     2581 cgcgtacacc gcggcgctcg ccggcttctc gatctgggcg cccaagttcc tgctgcgcgc
     2641 attcccaagc gagctggata tcgggagcgc caacctctac ctcggggcgg tgctgatggt
     2701 tgccggtacg atcgggatct ggatcggtgg ccgctatgcc aatcgcgcac tggtccatca
     2761 cagagttgcg cccgacgagc cgtacgactc gggccgcaac aaacgcgcgg tcaattcgct
     2821 gctgcggatc gccgcggtcg cgatggcgct gtccgccccg ctgtcggcgc tcggcttctt
     2881 ggtgccgggc gccgtcgcgt acttcgtgat ctcgttcgtg gtcgaaatct gcctgttcgc
     2941 gacgacctcg ccggtcaacg cggcgctgct gcgcgcggtc ccggccgagc gccgtgccag
     3001 cgcgatggcg gcaaccgtct tctcgatcca cctgttcggc gatctgtggt cggcggccgc
     3061 gctcggcctg ctgctcgaca acctgccgct caagatcgcg atgatgtcgc tgccgctgac
     3121 cttcgcgtgg tcggcgtaca tctggcggcc gcgccagcgc gaggccgagg cgccgtcggc
     3181 aaccggcccc gatccggtgc ccgaagcgcg cgtccacacg ggcacctgat cgccgcgccg
     3241 ccgtggtaca ccgcggggcg cgatgaagat ccacgagtat cagggcaagc aactgttccg
     3301 gaagtacggg gtgcccgtac cgatcggcat cccggcgatg acggtcgagg aggcgctcgc
     3361 ggcgatcccc aaggtgcagg ccgacgcggg caccgacgtg gtggtcgtca aggcgcagat
     3421 ccacgccggc ggccgcggca agggcggcgg ggtcaaggtc gccaagaccc ggaccgacgc
     3481 cgaggccgcg gtcaagaaga tcttcggcat gcagctggtc acgccgcaga ccggccccga
     3541 gggcaagaag gtccagcgcc tgctcgtcga gcaaggcctg gcgatcgagc acgagtacta
     3601 cctcggcatg ctgctcgacc gcgcgaccgg ccgggtcacg gtgatggcgt cgaccgaggg
     3661 cgggatggac atcgaggagg tcgccgccaa gcaccccgag aagatcctga agcacgcgat
     3721 cgaccccgcg accggctggc aggactggca gtcgcgccag cttgcccacg agctcgggat
     3781 gaccggcaag gctggcgagg cgctggccgg cttcctccgg ccgctgaccc ggtgctacga
     3841 cgacatggac tgctcgctgc tcgagatcaa cccgctgatc accacgaccg acggccgggt
     3901 gctcgcgctc gacgccaaga tcaacttcga cgacaacgcg ctgtaccgcc accccgagct
     3961 cgagaactgg cgcgatccca gcgaggagga cgcggcggag gccgaggcca agaagtggga
     4021 cctgtcgtac atctcgctcg acggcaacat cggctgcatg gtcaacggcg ccggcctggc
     4081 gatgtcgacc atggacatca tcaagttcta cggcggcgca ccggccaact tcctcgacgt
     4141 cggcggcggc gcgaccgcgg agaaggtcac cgcggcgttc aagatcatca cccgggatcc
     4201 cgacgtcaag gcgatcttcg tcaacatctt cggcggcatc atgaagtgcg acacgatcgc
     4261 gcagggcgtg atcgccgcgg tcaaggaggt cggcctccag gtcccgctgg tcgtccggct
     4321 cgagggcacc aacgtcgagc tcggcaagaa gatgctcgcc gagagcaagc tcaacatcgt
     4381 cgccgcggcc gacatggccg acggcgcgca gaaggtggtc aaggccgcca aggaggccag
     4441 gtgacatgag cgtgctggtc ggatacgaca cccggctc
//
LOCUS       DASVOR010001862         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_136387,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001862 DASVOR010000000
VERSION     DASVOR010001862.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136387"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1820)
                     /locus_tag="VF516_34625"
     CDS             complement(<1..1820)
                     /locus_tag="VF516_34625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112935.1"
                     /translation="MTRLVRDLGFAVSLALAACGGKSGDPPGGGPPVDAGTGAPDGMM
                     PDAAPSFTLTAIFPAAASRTVDSPLTISGMGITGTPSIRLTNCDQPGTGYDLTAGTTT
                     ASSVATTLAADPTRVQGAYTVTVTNGDGMTASLTCALHIVAEPPPTVSVIVPSTAWQG
                     SPTDNINSDATVSIEGTGFLSTPSVRWVLRSNPATYFDAPFVGFVSEGHLTAVAPSET
                     AAMPAGAYDVFVTNPDQLTAQWKVGMMAGTFTVTATPPPHITDVSPARVQNGSCTSTS
                     ITISGDSFATGSTAWYLAPAGTACTGSTTDASGNLLCPVAVDGVTATAITGHFAGCPA
                     LGPYPVVVVNADGQTSYWFSIEITPSSDGHLNVGSFETAQNHLEVARWKHAVQFGFDV
                     FSDALVYVAGGQDAQGNVLGSVEVSRFDLFGVPGPFHHLEQYGGAANPRIANDLTVPR
                     EGATLVRAGASLFAIGGTAARSDTTTVVAASDVVERAEILGFGQMPAIQHPAAQPQIQ
                     GLPSGAWYYRVSAVGPWGEGLASREAVALGASGQVKVCWQPVAGAASYNIYRSLASDG
                     RAGSAAAIAYEVTAADSCWLDTGIGDRAPAPGNARGTLAAG"
BASE COUNT          282 a          765 c          803 g          291 t
ORIGIN      
        1 cccgcggcga gcgtgccgcg cgcgttgccc ggtgcgggcg cgcgatcgcc gatgccggtg
       61 tcgagccagc agctgtcggc cgcggtcacc tcgtaggcga tcgccgccgc gctgccggcg
      121 cggccgtcgg aggccagcga gcgatagatg ttgtacgacg ccgcgccggc gaccggctgc
      181 cagcacacct tgacctggcc cgatgcgccg agcgcgaccg cctcgcggct ggccaggccc
      241 tcgccccacg ggccgaccgc ggacacccgg tagtaccagg cgccggacgg caggccctgg
      301 atctgcggct gcgccgcggg gtgctggatc gccggcatct ggccgaaccc gaggatctcg
      361 gcgcgctcga cgacgtccga cgcggcgacc accgtcgtgg tgtccgagcg cgcggccgtg
      421 ccgccgatcg cgaacaggct ggcgcccgcg cgcaccagcg tggcgccctc gcgcggcacc
      481 gtgagatcgt tggcgatccg cgggttcgcc gcgccgccgt actgctcgag gtgatggaac
      541 ggccccggca cgccgaacag gtcgaaccgg ctgacctcga ccgagccgag cacgttgccc
      601 tgcgcgtcct ggccgcccgc gacgtacacc agcgcgtcgg agaacacgtc gaagccgaac
      661 tgcaccgcgt gcttccagcg cgcgacctcc aggtggttct gcgcggtctc gaacgagccg
      721 acgttgaggt ggccgtccga cgatggcgtg atctcgatcg agaaccagta cgaggtctgg
      781 ccgtcggcgt tgaccacgac caccgggtac ggtccgagcg ccggacagcc ggcgaagtgg
      841 ccggtgatcg cggtggcggt gacgccatcg accgcgaccg ggcacagcag gttgccgctg
      901 gcgtcggtgg tcgagccggt gcacgccgtg ccggccggcg ccaggtacca cgcggtggac
      961 ccggtcgcga agctgtcgcc gctgatcgtg atcgaggtcg acgtgcacga gccgttctgg
     1021 acccgcgccg ggctgacgtc ggtgatgtgc ggcggcgggg tcgcggtgac cgtgaacgtc
     1081 ccggccatca tgccgacctt ccactgcgcg gtcagctgat cggggttggt gacgaacacg
     1141 tcgtaggccc ccgccggcat cgccgcggtc tcggacggcg cgaccgcggt gaggtgaccc
     1201 tccgacacga agccgacgaa cggggcgtcg aagtacgtcg ccggattgct gcgcagcacc
     1261 cagcgcacgc tcggcgtcga caggaacccg gtgccctcga tgctgaccgt cgcgtcggag
     1321 ttgatgttgt cggtcggcga gccctgccac gcggtgctcg gcacgatcac gctcaccgtg
     1381 ggcggcggct cggcgacgat gtgcagcgcg caggtcagcg acgcggtcat gccgtcgccg
     1441 ttggtcacgg tcacggtgta ggcgccctgg acccgcgtcg gatcggcggc gagcgtggtc
     1501 gcgaccgagc tcgcggtcgt ggtgcctgcg gtcagatcgt agccggtgcc gggttgatca
     1561 cagttggtca gccggatgct cggggtcccg gtgatcccca tgccgctgat cgtgagcggg
     1621 ctgtcgaccg tccgtgaggc cgcggccggg aagatcgcgg tcagcgtgaa gctgggcgcg
     1681 gcgtcgggca tcatgccgtc gggtgccccg gtgccggcgt cgaccggcgg gccgccgccg
     1741 ggcgggtcac cgctcttgcc gccacacgct gccagcgcga gcgagacggc gaatccgaga
     1801 tcgcgaacca gtcgagtcat catcgatcac acgcccagca acgcgggtgc catggcgcgc
     1861 gaccgccgcc gccgcgactt gcgaggcgct gtacgcggcg caaggcgtcc ggatcggtac
     1921 gccgcgtccc aaacggttcg cggggctgtc tcgcggtgtg gggcctcgct acactcgcgg
     1981 cgtgagcgtg ctcgccgcgg cgatcgccct ggccgatctg tgcggtgccg cgcccgcgcc
     2041 agcggcgagc cccgatccag ccgacagcgc cgcctacacc gcggtcggcg accaggcgcg
     2101 agcgtccggc gacgagcgca ccgcggcgat cgcgtaccgc c
//
LOCUS       DASVOR010001863         4492 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_195136,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001863 DASVOR010000000
VERSION     DASVOR010001863.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4492
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195136"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..970)
                     /locus_tag="VF516_34630"
     CDS             complement(146..970)
                     /locus_tag="VF516_34630"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEX8112936.1"
                     /translation="MAADWLTGVVELGGDRAPGLLWGASLALGVAYAVRSARRVELDP
                     RAAYWAAMLAVGCGLIGSRLLGMIVNGPDDDRSWSSLIDGGRSYYGGLLAGAAAALAY
                     LRVRRQPVLRHADALAPACALGYCVGRIGCFFNGDDYGVVVSGAFPLAVRYPPGTEAY
                     GAHLERGLISPADAMSLPVVPSQLGHAALGLILFLVLRRPRSVDGMRVASFALLYGIG
                     RFTLEFARGEFAAVVGPMSLHQVISLGLIGAGAAVLIRAASRRGVGMVAPAGVETL"
     gene            complement(970..3069)
                     /locus_tag="VF516_34635"
     CDS             complement(970..3069)
                     /locus_tag="VF516_34635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112937.1"
                     /translation="MRSDKWLKVLMGTMVLLPSANAWSAENRTFCFSPAFDAWRNFPA
                     PTIDGVIDFPDEKGWAPAFKYVFNNGTPDPDVVLYGYSDNASRLAMGVTVRNDTTYDD
                     TDAIVMLFDFGPSDGDGPRYGELIIHPLFGNITDQSGELPRENLGSGGQVYSNIYASY
                     FTTNNADNPDPNATWTSNPAVGTWLTVARKTTPDGTACAQGANHGTKCRWDVELLIDT
                     VAPGAPPVMSAQKIFVDVFRADRSAAGIPTLQFTWPQNLQDAKKNYVVGAPAGLSKFN
                     AGVAGQAGTPPTSIWGTVAVNTSTSCHGVWFRGMGNAISDITIDTGTITWGLPSFNQN
                     QTVTLDAHIHNSTVDGSTGKPIAATNVSATFYHAPFGMSGFNHFFLIPNNPGNSNDPD
                     VKNPTDPRKVDNDGAIDPATTANGTEFKLKWTPTTKCDPQNPSNSPANCDTDHQCMLV
                     MLSSPDNDTTFVNQAEFQNMWGNATASTFNGSARISVKGEPPPTGGGPQHVRLHGTRT
                     LQFAYSDGSNGIPSGTLTSQLKWLFHGARFTGDHITIKGIDHEVWVPVSSYGYLVQHE
                     LGLDVQNSFEQRHADLAKRIHGSGADGRPSPSVIREVNNALAVDPEQPPPSSWDLHVS
                     GGPGVSVGPDDVVDLELPLDGETMLHTVAVSTTTKPRIECFHCFGSSGSATTPSKVGT
                     LGVLALIGLVAYRRRRR"
     gene            complement(3106..4359)
                     /locus_tag="VF516_34640"
     CDS             complement(3106..4359)
                     /locus_tag="VF516_34640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112938.1"
                     /translation="MSISIQPPQPGDLITSSFVKQLIDQLKALDVRISALEGQAPGIG
                     GNLAIISLSASDLDIGAELRIFGVNFGLPSENVVTFDGQNPVRQFKPGSGDKLLILDV
                     PPLSFSGDARVFQVTVSNPRGFDQKLLTVHMPKLTIPTGTLTVGSPQFPAQKIVVPAS
                     GSVDATFGFTLTVSATMDEVYNVMPTYPQGWSAVLVTDMTGKTVLQQPPGQPAPPPWK
                     LPIPKPPNGLHDTIVPVFVRLTIPSTANPAVGGSVGLKVASVNNPEFTAHPFSVDFPL
                     NATLPNSQTLTISVAAVGGEDARSLDDTIFAVPTRATQDLDQVTFKVQKMSANTKYHV
                     KLAWQDGNSHGWTASLDGAGAMESPPNTPLVGPGDALVPVVIAGAATATDATLIFTVQ
                     KLPAVDPITTSTDYGIANQVIKPSK"
BASE COUNT          822 a         1437 c         1411 g          822 t
ORIGIN      
        1 gccgccggcg gcaagctgtc cggccgcagc tcgatcgcgc cgagcatgcg ctcgacggcc
       61 agccgcaccg ccagcggatc ggcgccctcg gcgcgcaccg ccagccgggc gatcgcggca
      121 tcgccggcgc tccggcgctg cgggatcaca gagtctccac ccccgcgggg gcgaccatac
      181 cgactcctcg ccgagacgcg gcgcgaatca gcacggccgc gccggcgccg atgagcccga
      241 ggctgatcac ctggtgcagc gacatcggac cgacgactgc cgcgaactca ccgcgggcaa
      301 attccagcgt gaagcggccg atcccgtaga gcagcgcgaa gctcgcgacg cgcatcccgt
      361 ccaccgatcg gggccggcgc aggacgagaa acaggatcag cccgagcgcg gcatgcccga
      421 gctgcgaggg caccaccggc agcgacatgg catccgccgg agagatcagc ccccgctcga
      481 gatgcgcccc gtaggcctcg gtacctggcg gataacgcac ggccagcgga aacgcacccg
      541 agaccaccac gccgtagtcg tcgccgttga agaagcagcc gatccgcccg acgcagtagc
      601 caagcgcgca cgccggggcc aatgcgtcgg cgtggcgcag caccggctgg cgacgcacgc
      661 gcaggtacgc gagcgcggca gccgcaccgg ccagcagccc gccataatag ctccggccgc
      721 catcgatcag cgagctccag ctccggtcat cgtcgggtcc attgacgatc atcccgagca
      781 ggcggctgcc gatcaggccg cacccgacgg cgagcatcgc ggcccagtac gccgcgcggg
      841 gatcgagctc gacgcggcgc gccgaacgca cggcgtatgc gacgcccagc gcgagcgagg
      901 cgccccacag caggccgggc gcacggtccc cgccgagctc gaccacgccg gtgagccagt
      961 ccgccgccat caccgccgcc gtcgccgata ggcgaccagc ccgatgagcg cgagcacgcc
     1021 cagcgtgccg accttcgacg gtgtggtcgc gctgccggac gacccgaagc agtgaaagca
     1081 ctcgatcctg ggcttcgtcg tcgtggagac cgcaacggtg tggagcatgg tctcgccatc
     1141 gagcggtagc tcgaggtcca ccacgtcatc tggaccgaca gagacgccgg gcccgccgct
     1201 cacatgcagg tcccacgagg acggcggcgg ctgctccggg tcgacggcca gcgcattgtt
     1261 gacctcccgg atgaccgacg gcgaagggcg gccatcggcc ccggacccat gaatccgctt
     1321 ggccagatcg gcgtgccgct gctcgaagct gttctgaaca tcgagcccca gttcatgctg
     1381 caccaggtac ccgtacgacg acaccggtac ccagacctcg tggtcgattc ccttgatggt
     1441 gatatgatcc ccggtgaacc gcgctccgtg gaacagccac ttcagctggg atgtcagcgt
     1501 tccactggga ataccattgc ttccatcaga ataggcaaat tgcagcgtgc gcgttccgtg
     1561 gaggcggacg tgctgggggc caccacccgt cggaggcggc tcgcccttca cgctgatccg
     1621 tgcagaacca ttgaaggtcg acgccgtcgc gttgccccac atgttctgga attccgcctg
     1681 attgacgaat gtcgtgtcgt tatcgggaga gctcaacatg accaacatgc actggtgatc
     1741 cgtatcgcaa ttcgcgggtg aattcgacgg attctgagga tcgcacttcg tggtaggggt
     1801 ccacttcagc ttgaactccg tgccgttggc ggtcgtggca ggatcgatgg cgccatcatt
     1861 atcaacctta cgtggatctg tcggattctt cacatcgggg tcattcgaat tcccaggatt
     1921 gttggggatc agaaagaagt gattgaatcc ggacataccg aacggcgcat gatagaacgt
     1981 cgccgatacg ttcgtcgccg cgatgggttt tccggtactt ccatccaccg tgctgttatg
     2041 aatatgtgca tcgagcgtga ccgtctggtt ttgattgaag cttggcagcc cccaggtgat
     2101 ggttccggtg tctatcgtga tatcgctgat tgcattcccc atgccgcgaa accagacccc
     2161 atgacaactg gtactcgtgt ttacagcaac ggtcccccag atcgaggtcg gcggggtacc
     2221 ggcctggcct gcgacgccag cgttgaactt gctgagtcca gccggtgcac ccactacata
     2281 gttctttttc gcatcctgca agttctgggg ccaggtgaat tgaagggtgg gtatccctgc
     2341 cgcgctgcga tcagcacgga acacgtcgac gaagatcttc tgggccgaca tcaccggcgg
     2401 ggcgccggga gcaaccgtat cgatcagcag ctcgacatcc caccggcatt tggtaccgtg
     2461 attggcgccc tgggcgcacg cggtgccgtc cggggtcgtc ttccttgcca ccgtcagcca
     2521 ggtaccgacc gctggattcg atgtccaggt cgcattggga tccggattgt ctgcgttatt
     2581 cgtggtaaaa tagctcgcat agatgttgct gtagacctga ccgccgcttc ccaggttctc
     2641 acgtggaagc tctccggatt gatctgtgat attcccgaac aacggatgaa tgatcagctc
     2701 tccgtatcgc ggcccatcgc catcactagg tccgaaatcg aacaacatca cgatggcatc
     2761 ggtatcatcg tacgtcgtat cattgcgaac ggtgactccc atggccagac ggctggcatt
     2821 atcagaatat ccgtacaaca ccacatccgg atcgggagta ccattgttga agacgtactt
     2881 gaaggccggc gcccaaccct tctcatcggg gaagtcgatg acgccatcga tcgtcggtgc
     2941 cgggaagttg cgccaggcat cgaaggctgg gctgaagcag aatgtgcgat tctccgcgga
     3001 ccaggcgttg gcgcttggca acaagaccat cgtccccatc aacactttca accatttgtc
     3061 gcttcgcatg aatgatttct ccctctgtgt cttcgatcag cctggctatt tggacggctt
     3121 gatcacttga ttggcaattc cataatccgt ggatgtcgtt atcggatcca ccgccggcag
     3181 cttctgcacg gtgaatatca gcgtcgcgtc ggtggcggtc gcggcgccag cgatgaccac
     3241 cggcaccagg gcatcgcccg gcccgaccag cggtgtgttg ggcggtgact ccatggcgcc
     3301 ggctccgtcc agggaggcag tccatccatg cgaattaccg tcctgccatg ccagtttcac
     3361 atggtacttg gtattcgcgc tcatcttctg gaccttgaac gtgacctgat cgaggtcctg
     3421 cgtcgcgcgc gtgggcacgg cgaagatcgt gtcgtccaac gatcgagcat cttcgccgcc
     3481 gacggcggca acgctgatcg tgagtgtttg actgttcggc agcgtcgcat tgagcgggaa
     3541 gtcgaccgag aacggatgag cggtaaactc cgggttgttc accgacgcga ctttcagccc
     3601 cacgctccct ccgaccgccg gattggccgt cgacgggatc gtcagccgga cgaagaccgg
     3661 tacgatggtg tcgtgcagtc cattcggcgg cttgggaatc ggcagcttcc atggtggcgg
     3721 cgccggctga cccggcggct gctgtagcac cgtcttgccg gtcatatcgg tgaccagcac
     3781 agcactccaa ccttgcggat aggttggcat cacgttgtag acctcgtcca tcgtggcact
     3841 cacggtgagc gtgaatccga acgtggcatc gacggaaccg cttgccggga cgacgatctt
     3901 ctgggccgga aattgcggac tccccaccgt cagtgtcccg gtgggaatcg tcagtttcgg
     3961 catgtgcacg gtgagcagct tctggtcgaa cccacgaggg ttgctgaccg tgacctggaa
     4021 cacccgggcg tctccggaga acgagagcgg tgggacgtcc aggatcagca gcttgtcgcc
     4081 gctgcccggc ttgaactgcc tgaccgggtt ctggccgtcg aacgtcacca cgttctccga
     4141 gggcagaccg aagttcacgc cgaagatccg gagctcggcg ccgatgtcga gatcgctggc
     4201 cgacagactg atgatcgcga ggttgcctcc gatcccggga gcctggcctt cgagggcgga
     4261 gatccgcacg tcgagcgcct tgagttggtc gatcagctgc ttgacgaagc tgctcgtgat
     4321 gaggtcgccc ggttggggtg gttgaatcga gatcgacatt gcgtcctcct gcgctcacgg
     4381 gctcatgcgc tcgtcgtcga gcgcgtagtt ggcaaagtcc cagtgcgaca cgtcgaagcg
     4441 cggcgccgcc tggtacggca ggcggccgag cgcgaagatc aggtacggct gc
//
LOCUS       DASVOR010001864         1517 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_20708,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001864 DASVOR010000000
VERSION     DASVOR010001864.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20708"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..>1517
                     /locus_tag="VF516_34645"
     CDS             229..>1517
                     /locus_tag="VF516_34645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112939.1"
                     /translation="MFAVPGCVASDATTGEPEQVATELVQPLDPDPATVCSTDPRVVA
                     GVVTATTCIGANLFFRGTFGGNGRTCATCHRVDNNLTIDPKFIATLPATDPLFVAEFN
                     SDLKDLEKPPQMHTFSLILENVDGFDPDPRTHFVLRSVPHTLSLSTSVTLPPGGVNPP
                     ADRTGWSGDGAPGTGALRDFMTGAIRQHYTKTLARVAGTDFQLASDTQLDQIDQFMRR
                     IGRSNELALSTVVMSDAGADAGRNKFLAVGCNACHGNAGANAGGGNRNFNTGVESSRN
                     SALGAFPHDGGFGTVASTTGGPFGDGTFNVPPLVEAADTGPFFHTATSIVGAPAHNVA
                     TATTIEEAIAFYTTAAFRNAPDGFPIALTATDIDNVGRFLRGINAAFSAAIAIKRCDA
                     ELAIIAQFHNTQIAIQNELIRLANVEVGDAIRVLSEV"
BASE COUNT          266 a          557 c          461 g          233 t
ORIGIN      
        1 accgagcatc gccgacctca cacggcgatc acggtcagcg cactgccaac cacgagcctg
       61 ccagtctgca aatcccagga tgggtctcac gatctgacga tcggatttaa ccacttccga
      121 tatccaggat tatttatcct cgctgcccgc gatcaggcag tccatgatcg atcacagtag
      181 tcaggagtaa gcatgaggta ccatcgttgt ttcgtcttga tcacaggcgt gttcgccgta
      241 cccgggtgcg tcgcatccga cgccaccacc ggcgagccgg agcaggtggc cacggagctc
      301 gtccagccac tcgaccccga cccggccacg gtctgctcga ccgacccacg ggtcgtcgcc
      361 ggggtggtga ccgccaccac gtgcatcggt gccaacctgt tcttccgcgg aacgttcggc
      421 ggcaacgggc ggacctgcgc gacctgccac cgggtcgaca acaacctcac gatcgatccg
      481 aagttcatcg cgacgctgcc ggccaccgac ccgctgttcg tggccgagtt caattcggat
      541 ctcaaggacc tcgagaagcc cccgcagatg cataccttca gcctgatcct cgagaacgtg
      601 gacggcttcg atcccgaccc gcgcacccac ttcgtgctgc gcagcgtgcc gcacacgctg
      661 tcgctctcga ccagcgtcac gctgcccccc ggcggcgtca acccgcccgc cgaccgcacc
      721 gggtggtccg gcgacggcgc cccggggaca ggcgcactgc gcgacttcat gaccggcgcg
      781 atccggcagc actacaccaa gaccctcgcc cgcgtggcgg ggacggactt ccagctggcc
      841 agcgacaccc agctcgacca gatcgatcag ttcatgcgac ggatcggccg cagcaacgag
      901 ctggcgctct cgaccgtggt gatgtccgac gctggcgccg atgcggggcg taacaagttc
      961 ctggcggtcg ggtgcaacgc ctgccacggc aacgcgggcg ccaatgcggg cggtgggaat
     1021 cgcaacttca ataccggcgt ggagtcgtcg cgcaactcgg cgctcggcgc gttcccgcac
     1081 gacggaggtt tcgggaccgt cgcctccacg acgggcggcc cgttcggcga tggtacgttc
     1141 aacgtgccgc cgctggtcga ggccgcggac accggcccgt tcttccacac cgccacgtcg
     1201 atcgtcgggg cgcccgccca caacgtggcc acggcgacca cgatcgagga agcgatcgcg
     1261 ttctatacca ccgcggcgtt ccgcaacgcg cccgacggct tcccgatcgc cctcacggcg
     1321 accgacatcg acaacgtcgg ccggttcctg cgcggcatca acgcggcgtt cagcgccgcg
     1381 atcgcgatca agcgctgcga cgccgagctc gcgatcatcg cccagttcca caacacccag
     1441 atcgcgatcc agaacgagct gatccggctc gcgaacgtcg aggtcggcga cgcgatccgc
     1501 gtgctgtccg aggtgca
//
LOCUS       DASVOR010001865         1514 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_515907,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001865 DASVOR010000000
VERSION     DASVOR010001865.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1514
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515907"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VF516_34650"
     CDS             <1..292
                     /locus_tag="VF516_34650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112940.1"
                     /translation="IEANVCERFYACFTPAELAAASFPADEAACVSQMQATKGCANQT
                     TASACVGNQHYHPDQAATCANQISGLTCGQIRDQSANLDADAPACDKICAVD"
     gene            complement(312..938)
                     /gene="clpP"
                     /locus_tag="VF516_34655"
     CDS             complement(312..938)
                     /gene="clpP"
                     /locus_tag="VF516_34655"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX8112941.1"
                     /translation="MKYRGDIQSAGPVALIPTVIEQTHRGERGWDLYSRLLKDRIVFL
                     GTEIDAGVANVVIAQLLFLDSEDPKRDIMLYVNSPGGDVSAGLAIYDTMQHVHCDVAT
                     VCMGLAASMGSFLLATGTRGKRYALPHARVMIHQPLAGFQGPATDIEIHAREVLLARD
                     TINDLYARHTGQPVERIKHDTERDNFMSAAEARDYGLIDEVLTTLKKS"
     gene            complement(935..1366)
                     /locus_tag="VF516_34660"
     CDS             complement(935..1366)
                     /locus_tag="VF516_34660"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8112942.1"
                     /translation="MSKKTPPEAALYVRLPATAVDKLDRAAEALGVHKKDLVAGLVSK
                     YVDPDTRQGLSALGSLSQPRRVTFDLGEPSPTLGTYSFQPYDPPEVMNAEQAAQFLQI
                     DEKVVIELAEAGKLPGRKLGPAWRFSRAALVAWLAGPEKPR"
BASE COUNT          233 a          509 c          536 g          236 t
ORIGIN      
        1 catcgaggcg aatgtctgcg agcggttcta tgcgtgcttc acgccggcgg agctggcggc
       61 ggccagcttc ccggcggacg aggccgcgtg cgtgtcgcag atgcaggcga cgaagggctg
      121 cgcgaaccag acgaccgcca gcgcctgcgt cggcaaccag cactatcatc cggaccaggc
      181 cgccacgtgc gcgaaccaga tctccgggct gacatgcgga cagatccgcg accagagcgc
      241 gaacctcgac gcggacgcgc ccgcgtgcga caagatctgc gcggtcgact gagcggtggc
      301 cgtgtcgccg gctacgactt cttcaaggtc gtgagcacct cgtcgatcaa gccgtagtcc
      361 ctggcctccg ccgccgacat gaagttgtcg cgctcggtgt cgtgcttgat gcgctcgacc
      421 ggctgaccgg tgtgccgggc gtacagatcg ttgatcgtgt cgcgcgcgag caggacctcg
      481 cgggcgtgga tctcgatgtc ggtcgccggg ccctggaagc cggcgagcgg ctggtggatc
      541 atcacgcggg cgtgcggcag cgcgtagcgc ttgcccctgg tgccggtcgc gagcaggaag
      601 ctgcccatcg acgccgccag gcccatgcac accgtcgcga cgtcgcagtg cacgtgctgc
      661 atggtgtcgt agatcgcgag gcccgcggac acgtcgccgc cgggcgagtt gacgtacagc
      721 atgatgtcgc gcttgggatc ctccgagtcg aggaacagga gctgcgcgat caccacgttg
      781 gccacgccgg cgtcgatctc ggtgccgagg aacacgatcc ggtccttgag cagccgcgag
      841 tacaggtccc agccgcgctc gccgcgatgg gtctgctcga tcacggtcgg gatcagcgcg
      901 accggaccgg cgctttgaat atctccgcga tatttcatcg tggcttctcc ggcccggcga
      961 gccaggcgac cagggccgcg cgcgagaacc gccacgcggg gccgagcttg cggccgggca
     1021 gcttgccggc ctcggcgagc tcgatcacga ccttctcgtc gatctgcagg aactgggcgg
     1081 cctgctcggc gttcatcacc tccggcgggt cgtagggctg gaacgagtag gtcccgagcg
     1141 tcgggctcgg ctcgccgaga tcgaacgtga cccggcgcgg ctgcgacagc gagccgagcg
     1201 cgctcagccc ctgccgggtg tcgggatcga cgtacttcga gaccaggccg gcgaccagat
     1261 ccttcttgtg cacgccgagc gcctcggcgg cgcgatcgag cttgtcgacc gcggtcgcgg
     1321 gcagccggac atagagcgcc gcctccggcg gtgtcttctt cgacatatcc gcaatatatc
     1381 cagaaaatat tcgatgtcaa gccggacgcc ggggcaggtc acgcctcgtg gtcgccgtgg
     1441 tcgaccagcg cgtacggcca cgactcgacc ccggcctgga gcacctcggc gggaggcggc
     1501 gcggtcgcgg gcag
//
LOCUS       DASVOR010001866         3996 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_370312,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001866 DASVOR010000000
VERSION     DASVOR010001866.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370312"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1383
                     /gene="pepQ"
                     /locus_tag="VF516_34665"
     CDS             88..1383
                     /gene="pepQ"
                     /locus_tag="VF516_34665"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: protein motif:HMM:NF010133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HEX8112943.1"
                     /translation="MTFAATYPAHLEYLQRGAEAALAAHGFDAVVLCSGAAASRNRFD
                     DQYWPVMPTPAYAHWCPLVEADAFVVVRPGARPRLVRTIVDDFWEAAALPESNHFWSC
                     FELVEVRPGHAADALPGGNVAVITRDPGASPPGTVNPPDLIAALDALRTRKTAYEIEC
                     LAEASRRAVRGHRRAAELFRAGVPSELDLHLAYLAASEQDDLWAPYKGIVALGSHAAV
                     LHFVAYTRERVTGDTSLLVDAGARCLGYGSDITRTTVRGDGAAARRFAELLARMEALQ
                     QEIVRRIRPGLPYEDLHDDSHRLLAVVLRELGIGRGSPDELVERGVTRALYPHGLGHS
                     LGVTVHDVGLKPRPPRPENRFLRNTSVIEPGQVFTIEPGIYIIDPLLAPLQADDRRDL
                     VDWAAIAELRPFGGIRVEDNVLVQDRGVRNLTREAFAEA"
     gene            1444..2730
                     /locus_tag="VF516_34670"
     CDS             1444..2730
                     /locus_tag="VF516_34670"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8112944.1"
                     /translation="MRGSVLLFFSMGLLACHSASDDKPVSNTAPEPSQVGSGSGGIRK
                     HQVPPPFDLKTPPGDATKTASGLVYKKLVTNAAGDSPKRNDTVMINYTGWRQSTGETF
                     FTNQSRGQPMPLKLSQSAPGFVEAMQLLHTGEKAVLWIPPEIGYKTKPEGGKPDTLVY
                     EVEVVEVQPAPPIPDDVGAPPAKATALKSGTKFVLVRPGTGKENVRMFDTVSYKFTVW
                     DGTGRMLDTNEVGNAHAMTVQLYKQPAAMAEMVTSLPVGARGRFWIDAEKIVAGGNKP
                     PGGVEHGPLCYEIEITQNTKAAAEPPPTPPDVAKPPADAQKTAKGVFYRFVAHGPGKD
                     PRHPTPKDTVKTHYTGWTTDGKMFDSSYMRNEPSTFKLTGVIAGWTDGLQVMTPGDRV
                     RFWIPEELAYKGQAGKPQGMLVFEIELLEIAKTDAN"
     gene            2734..3756
                     /locus_tag="VF516_34675"
     CDS             2734..3756
                     /locus_tag="VF516_34675"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8112945.1"
                     /translation="MVPDDVAAAWGWADPSVEIAPLTGGLINATYAVRRGGEPVAVVQ
                     RLHPIFAGEVNLDIDAVTSHLAARGLPTPRLIRTRDGRAWHEHGGVWRALTWVDGETV
                     HAVPGPAWAEAGGALVGAFHRAVADLSHDYAFARVGVHDTTAHLARLADCVAAGGDPA
                     AVALGREILDAARALPDMPAVPRRHCHGDLKISNVLFAIAPLRGMCLVDLDTVGLSTM
                     AFELGDAMRSWCNPHGEDAGRVAFDLAIFAAAIRGFRDAADPVITADEKRAIVIGLET
                     VCLELAARFAVDVFRDEYFGWDPARFPSRRAHNLVRAQGQLDLGRAVRAARAEALDLV
                     HRARPS"
     gene            3764..>3996
                     /locus_tag="VF516_34680"
     CDS             3764..>3996
                     /locus_tag="VF516_34680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112946.1"
                     /translation="MNIITLSDSYDWLSPAIALRGRRQVRSNRWMQLRGATALVATLL
                     VAAAADASAPRSVPGMRADALTAMPAAGLARPLR"
BASE COUNT          566 a         1471 c         1408 g          551 t
ORIGIN      
        1 ccggggtgag cgagcctggg tgagcgggcc tggccacgcg gtgcttcgcc ggtgtcgcgc
       61 ccgcggccgt cgcggtaggc tcgacccatg accttcgcag ccacctaccc ggcccacctg
      121 gagtacctgc agcgcggcgc cgaggccgcg ctcgccgcgc acggcttcga cgccgtcgtg
      181 ctgtgcagcg gcgcggcggc gtcgcgcaac cggttcgacg accagtactg gccggtgatg
      241 ccgaccccgg cgtacgcgca ctggtgcccg ctggtcgagg ccgacgcgtt cgtcgtggtc
      301 cggcccggcg cgcggccgag gctggtgcgc accatcgtcg acgatttctg ggaggccgcg
      361 gcgctgcccg agagcaacca cttctggtcg tgcttcgagc tcgtcgaggt ccgcccgggc
      421 cacgccgccg acgcgctgcc cggcggcaac gtcgcggtga tcacgcgcga ccctggcgca
      481 tcgccgcccg gcacggtgaa cccgccggat ctgatcgccg cgctcgacgc gctgcggacc
      541 cgcaagaccg cgtacgagat cgaatgtctg gccgaggcga gccgccgcgc ggtccgcggc
      601 caccgccgcg ccgccgagct gtttcgcgcc ggggtgccgt cggagctcga cctccacctc
      661 gcctacctcg cggcctcgga gcaggacgac ctgtgggcgc cgtacaaggg catcgtggcg
      721 ctcggcagcc acgccgccgt gctgcacttc gtcgcgtaca cccgcgagcg cgtgaccggc
      781 gacacctcgc tgctggtcga cgccggcgcc aggtgcctgg gctacggcag cgacatcacg
      841 cgcaccaccg tccgcggcga cggtgcggcg gcgcgccggt tcgccgagct gctcgcccgc
      901 atggaggcgc tgcagcagga gatcgtccgc cggatccggc cgggcctgcc ctacgaggac
      961 ctccacgacg actcgcatcg cctgctcgcg gtggtgctgc gcgagctcgg gatcggacgg
     1021 gggagccccg acgagctggt cgagcgcggc gtgacccggg cgctgtaccc gcacggcctg
     1081 ggccactcgc tcggcgtcac cgtgcacgac gtcggcctga agccccgccc gccgcggccc
     1141 gagaacaggt tcctgcgcaa cacctcggtg atcgagcccg gccaggtgtt cacgatcgag
     1201 ccggggatct acatcatcga cccgctgctc gcgccgctcc aggccgacga ccgccgcgat
     1261 ctcgtcgact gggccgcgat cgccgagctg cggccgttcg gcgggatccg ggtcgaagat
     1321 aacgtgctgg ttcaggaccg cggcgttcgc aacctcaccc gggaggcgtt cgcggaggcg
     1381 tgacgatcac ggcctgcgca cgactggcgc ggcagggtcg gctgccgtat gttcggcccc
     1441 gccatgcgtg ggtcagtcct cttgtttttc tcgatgggat tgctggcgtg tcactccgcc
     1501 agcgatgaca agccggtcag caacaccgcc ccggagccga gccaggtcgg ctcgggctcg
     1561 ggtgggatcc gcaagcacca ggtcccgccg ccgttcgacc tgaagacgcc gcccggcgat
     1621 gcgaccaaga ccgcatcggg cctggtctac aagaagctcg tcaccaacgc cgcgggtgac
     1681 tcgcccaagc gcaacgacac ggtgatgatc aactacacgg gctggcgcca gtcgaccggc
     1741 gagacgttct tcaccaacca gagccgcggc cagccgatgc cgctcaagct gtcgcagtcc
     1801 gcgccgggct tcgtcgaggc gatgcagctg ctgcacaccg gcgagaaggc cgtgctgtgg
     1861 atcccaccgg agatcggcta caagacgaag ccggagggtg gcaaacccga cacgctggtc
     1921 tacgaggtcg aggtcgtcga ggtccagccg gcgccgccga tccccgacga cgtcggcgcg
     1981 ccgcccgcca aggcgacggc gctcaagtcg ggcaccaagt tcgtgctcgt ccgtcccggc
     2041 accggcaagg agaacgtccg gatgttcgac accgtgagct acaagttcac cgtgtgggac
     2101 ggcaccggcc gcatgctcga caccaacgag gtcggcaacg cgcacgccat gaccgtgcag
     2161 ctgtacaagc agcctgccgc gatggccgag atggtgacca gcctgcccgt cggggcgcgc
     2221 ggccggttct ggatcgacgc cgagaagatc gtcgccggcg ggaacaagcc acccggcggc
     2281 gtcgagcacg gccccctgtg ctacgagatc gagatcaccc agaacaccaa ggcggcggcc
     2341 gagcccccgc cgacgccgcc cgacgtggcc aagccgccgg cggacgccca gaagaccgcc
     2401 aagggcgtgt tctaccgctt cgtcgcccac ggcccgggca aggatccgcg gcatccgacc
     2461 ccgaaggaca ccgtcaagac gcactacacc ggctggacga ccgacggcaa gatgttcgac
     2521 tcgtcgtaca tgcgcaacga gccgtcgacg ttcaagctga ccggcgtgat cgccggctgg
     2581 accgacggcc tccaggtgat gacccccggc gaccgcgtgc ggttctggat cccggaggag
     2641 ctcgcctaca agggccaggc cggcaagccg cagggcatgc tggtgttcga gatcgagctg
     2701 ctcgagatcg cgaagacgga cgccaactga cgcatggtcc ccgatgacgt ggccgccgcg
     2761 tggggctggg ccgatccatc ggtcgagatc gcgccgctca cgggcggcct gatcaacgcg
     2821 acctacgcgg tgcgccgcgg cggcgagccg gtcgcggtcg tgcagcggct gcacccgatc
     2881 ttcgccggcg aggtcaacct cgacatcgac gcggtcacgt ctcacctggc ggcgcggggc
     2941 cttcccaccc cgcggctgat ccgcacccgc gacggccgcg cctggcacga gcatggcggg
     3001 gtgtggcggg cgttgacctg ggtcgacggc gagaccgtgc acgcggtgcc gggcccggcg
     3061 tgggccgagg ccggcggcgc gctggtcggc gcgtttcacc gcgccgtcgc cgacctgtcg
     3121 cacgactatg cgttcgcccg ggtcggagtc catgacacca ccgcccacct cgcgcggctc
     3181 gccgactgcg tcgccgccgg cggcgatccc gcggcggtcg cgctcggccg cgagatcctc
     3241 gacgcagcgc gcgcgctgcc ggacatgccc gcggtgccgc gccggcactg ccacggcgat
     3301 ctcaagatct cgaatgtcct gttcgcgatc gcgccgctgc gcggcatgtg cctggtcgac
     3361 ctcgacaccg tcgggctgtc gaccatggcg ttcgagctcg gcgacgcgat gcggtcgtgg
     3421 tgcaacccgc acggcgagga cgccggccgc gtcgcgttcg atctcgcgat cttcgccgcc
     3481 gcgatccgcg ggttccgcga cgccgccgac ccggtgatca cggccgacga gaagcgcgcg
     3541 atcgtcatcg gcctggagac cgtgtgcctc gagctcgcgg cgcggttcgc ggtcgacgtg
     3601 ttccgcgacg agtacttcgg ctgggacccg gcgcggtttc cgtcgcgccg cgcccacaac
     3661 ctggtgcgcg cgcagggtca gctcgacctg gggcgggcgg tccgcgccgc gcgcgccgaa
     3721 gcgctcgatc tcgtacatcg cgcgaggccg tcttgaccat ggcgtgaata tcatcacgtt
     3781 gtcggacagt tacgattggc tgtcgcccgc gatcgcactt cgcggccggc gccaggtgcg
     3841 ctctaataga tggatgcaac ttcgcggtgc gaccgcactg gtcgcaaccc tcctggtggc
     3901 tgccgcggcc gacgcgtcgg ccccgcgctc ggtgccgggc atgcgcgccg acgcgttgac
     3961 cgcgatgccg gccgccggcc tcgccaggcc gctgcg
//
LOCUS       DASVOR010001867         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_545779,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001867 DASVOR010000000
VERSION     DASVOR010001867.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545779"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VF516_34685"
     CDS             <1..1129
                     /locus_tag="VF516_34685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8112947.1"
                     /translation="PPGPPMPDTGSAGSAGSSGPPGPPGPGRPPFDYRPMPGRRMIAR
                     HHPVVLLFPVHGGRGVLVARGVHGAPPGLTGPLLALICGFLILVIGALITARWIVRPI
                     ERLSRTARALGSGDLQARSQLDRPDEIGELGHRFDEMADRIAGLLATEKELLANGPHE
                     LRTPLTRIGDALDLASEGDAEAARASLAEIAVDVSELETIVDDILTAMRFELASARAP
                     AQLPLRRARIPAAEIATAAADRLRARHPERPLALDSPDDLPAIDVDPVLLRRVIDNLL
                     ENAHKYTPDASSPIRLAVRRDAGDVVFEVHDRGIGIPADDLPRIFTAFFRGERSRSRE
                     TGGVGLGLTLARRIVEAHGGTIDVTSTPNVGTTVRVAIPAH"
     gene            complement(1261..>2449)
                     /locus_tag="VF516_34690"
     CDS             complement(1261..>2449)
                     /locus_tag="VF516_34690"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF039236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX8112948.1"
                     /translation="VDVPVHVDDSSSCGHGSWRSHVGDIAMQESGSSECSTVRTAIDA
                     SSRDLRRVDIHPDHWYPLALSHEVKRGKAHGVCFAGEPIVLVRTASGTVFALEDRCAH
                     RQIPLHDGVVDGETIRCCYHGWTYDCTGRCVNIPYIGRERLPNGVRAYPCREIAGLVF
                     VFPGDPALADSTPLPSLGSAGSSAYKTRRYAREIACHYSFMHENLMDMNHQFLHRRVM
                     GQIRARTLGRRHGDDWVEVDYTLAREKGRRPLGEAVVFRRGPRAPSTSGDVMTIRTSY
                     PYQTLQIRTLDARLMLDLWAVYVPLDREQRTTRAYGLVSIKRPRVRVLLDLAWPLLVW
                     FTERIFKEDRWAVEREQEAHDAQGADWNREVFPVINELRNLLRRRGAPPPGGGIALET
                     QQP"
BASE COUNT          360 a          931 c          809 g          349 t
ORIGIN      
        1 cccgccggga ccaccgatgc cggacaccgg ctcggccggc tcggccggct cgtccggccc
       61 gcccggcccc cccggccccg gccgcccgcc gttcgactac cggccgatgc ccggccggcg
      121 gatgatcgcg cgccaccatc ccgtggtgct gctgttcccc gtccacggcg gccgcggcgt
      181 gctggtcgcg cgcggtgtcc acggcgcacc tccggggctg accggcccgc tgctcgcgct
      241 gatctgcggc ttcctgatcc tggtgatcgg cgcgctgatc accgcgcgct ggatcgtccg
      301 accgatcgag cggctgtcgc ggaccgcccg cgcgctcggc tcgggcgacc tccaggcccg
      361 cagccagctc gaccgccccg acgagatcgg cgagctcggc caccgcttcg acgagatggc
      421 cgaccggatc gcgggcctgc tcgcgaccga gaaggagctc ctggccaacg gcccccacga
      481 gctgcgcacc ccgctgaccc ggatcggcga cgcgctcgac ctcgccagcg agggcgacgc
      541 cgaggccgcg cgggcctcgc tcgccgagat cgccgtcgac gtctccgagc tggagaccat
      601 cgtcgacgac atcctcaccg cgatgcggtt cgagctcgcg tcggcccggg cgcccgccca
      661 gctgccgctg cgtcgcgccc ggatcccggc ggcggagatc gccacggccg cggccgaccg
      721 gctgcgcgcg cgtcaccccg agcgcccgct cgcgctcgac agccccgacg acctgcccgc
      781 gatcgacgtc gatcccgtgc tgctgcgccg cgtgatcgac aacctgctgg agaacgccca
      841 caagtacacg cccgacgcca gctcgccgat ccggctcgcg gtccgccgcg acgccggcga
      901 cgtcgtgttc gaggtccacg accgcggcat cggcatcccg gccgacgacc tgccccggat
      961 cttcaccgcg ttcttccgcg gcgagcgcag ccgatcgcgc gagaccggcg gggtcggcct
     1021 cggcctcacc ctcgcccgcc gcatcgtcga ggcgcacggc ggcacgatcg acgtgaccag
     1081 cacgccgaac gtcggcacca cggtgcgcgt cgcgatcccg gcccattagc gcgatcgttc
     1141 agatatccgt ttgcggtctc ctgctcggcg atcgccgcga accacggatc gccggatgcg
     1201 gccggcaggt cggctcgtct gccacgccat atcggacgtg ggcccccggc cggccccgcg
     1261 ctacggttgc tgcgtctcca gagcaatccc gccccccggc ggcggcgcac cgcgacgccg
     1321 caacaggttg cgcaattcgt tgatcaccgg aaaaacctcg cgattccagt cagcgccttg
     1381 cgcgtcatgc gcttcctgtt cgcgctcgac ggcccagcgg tcttccttga agatgcgctc
     1441 ggtgaaccag acgagcagcg gccacgcgag atccagcagc acacgcacgc gcggccgctt
     1501 gatcgacacg aggccatacg cgcgcgtcgt gcgctgttcg cgatcgagcg gcacgtacac
     1561 ggcccacaga tcgagcatga gacgtgcgtc caacgtacgg atctgcagcg tctgatacgg
     1621 atagctggtg cggatcgtca tcacgtcgcc gctcgtcgac ggcgcccgcg ggccgcgcct
     1681 gaacaccact gcttcgccca gcggccggcg gcccttctcg cgcgcaagcg tgtagtcgac
     1741 ttcgacccag tcgtcgccat ggcgccggcc gagcgtgcgc gcgcggatct gccccatcac
     1801 gcggcggtgc aggaactgat ggttcatgtc catcaggttc tcgtgcatga acgagtagtg
     1861 gcaggcgatc tcgcgggcgt agcggcgcgt cttgtatgcg ctgctcccgg cagagccgag
     1921 cgacggcagc ggcgtcgaat ccgccagcgc cgggtcgccg gggaacacga agacgaggcc
     1981 ggcgatctcg cggcacggat acgcgcgcac gccattgggt aagcgctcgc ggccgatata
     2041 tggaatgttc acgcaacggc cggtgcagtc gtaggtccag ccgtggtaac agcagcggat
     2101 cgtttcgccg tccaccacgc cgtcgtgcag cggaatctgc cggtgcgcgc agcgatcctc
     2161 gagtgcgaac acggtgccag atgccgtgcg cacgagcacg atcggctcgc ccgcgaaaca
     2221 cacgccgtgc gccttgccgc gcttcacctc gtgcgacaac gcaagcgggt accaatggtc
     2281 cggatggatg tccacgcgac gcagatcgcg cgacgatgcg tcgatggcag tgcggacggt
     2341 gctgcattcg gatgatccgg actcttgcat ggcgatatcc ccgacgtgcg acctccacga
     2401 accatgtccg catgacgacg agtcgtcgac atgaaccggt acatcgacc
//
LOCUS       DASVOR010001868         1562 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_427994,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001868 DASVOR010000000
VERSION     DASVOR010001868.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1562
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427994"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VF516_34695"
     CDS             <1..525
                     /locus_tag="VF516_34695"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8112949.1"
                     /translation="QRGYEATSMQEIAEACGLTKAGLYHHIKTKEALLLAIMHYGMDM
                     FEDLVLAKAGDIVDPVARLRECMARNIALVTQDASKEVTIILHEHQTLTGAAAQEINE
                     RKKRYVRFLEAAFREAAERGQIRPVDPTLAAFSFLGMVLWIYKWYRADGKLSPREVSD
                     GMIDLFFEGLMPRS"
     gene            complement(893..1126)
                     /locus_tag="VF516_34700"
     CDS             complement(893..1126)
                     /locus_tag="VF516_34700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112950.1"
                     /translation="MPLRIPAVLTFTVLGAAAGVAAAVSCHGDGAPPPDAMLCSVQCI
                     PSGVGSNGCTPPTCATGPNHDVCPIGCTPDPIA"
     gene            1265..>1562
                     /locus_tag="VF516_34705"
     CDS             1265..>1562
                     /locus_tag="VF516_34705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112951.1"
                     /translation="MTDEPVRVRAPVVKIVDHGASTTLLGRALVRRFDGDSAALLRAV
                     LEIHARPVGRRELFAELARRAGTAPDELPAQPIGHTSWLGPVAQVGGVQPLLPTP"
BASE COUNT          220 a          518 c          610 g          214 t
ORIGIN      
        1 cagcgcgggt acgaggcgac cagcatgcaa gagatcgccg aggcctgcgg actgaccaag
       61 gccgggctgt accaccacat caagaccaag gaagcgctgc tcctcgcgat catgcattac
      121 ggcatggaca tgttcgagga cctggtgctc gccaaggccg gcgatatcgt cgatccggtc
      181 gcgcggctgc gcgagtgcat ggcgcgcaac atcgcgctgg tcacccagga cgcgagcaag
      241 gaagtcacga tcatcctgca cgagcaccag acgctgaccg gcgcggccgc gcaggagatc
      301 aacgagcgca agaagcgcta cgtgcggttc ctcgaggcgg cgttccgcga ggcggccgag
      361 cgcggccaga tccgcccggt cgatccgacg ctggcggcgt tcagcttcct cggcatggtg
      421 ctgtggatct acaagtggta ccgggccgac ggcaagctca gcccccgcga ggtgtccgac
      481 ggcatgatcg acctgttctt cgaaggcctg atgccgcgat cgtgatcgct cagtgccggg
      541 aactgctccg gtagctcgat gcggtccgtc cgacgcccag tccggggtcc gggctcaccg
      601 gcggttcggg cgagaccggc gcgccatggc tcgatgccgg cggccggcga ggatgggtgt
      661 gctggccgcc ggtcacgccg gcgaggtcgt cgaggctgag ggtgggcaga gcagcgggca
      721 gagcagcggg catggtgggc cttggtgagg gtgcagctcc tgctccagca cgcggcgtgc
      781 cggtgcgcgc aggccccggc tcgccagggg ttgccccgcg cgcggttgcc gcccgcggta
      841 gacacccggg ggctcgcgtc gccagccgct cgaccgagcg gcgggggcgt catcaagcga
      901 tcggatcggg cgtgcagccg atcgggcaca cgtcgtggtt gggcccggtg gcacaggtcg
      961 gcggcgtgca gccgttgctg cccacgccgc tcgggatgca ctgcacggag cacagcatcg
     1021 cgtcgggtgg cggcgcgccg tcaccgtggc agctcacggc ggctgccacc ccggcagcgg
     1081 cgccgaggac ggtgaacgtg agcacggccg ggatgcgcag cggcatggcc gcaccgtagc
     1141 gcatcgtcgg ctgcgcggcg ccgggatcca cgcatcgatg tcgatccgca cccgtgcgtt
     1201 gtggcgacag tcgcagagtc ggcgggcgcc cggccggcgg tcggagatcg cgtatgatcg
     1261 gcacgtgacc gacgagcctg tgcgcgtgcg tgcgccggtc gtgaagatcg tcgatcacgg
     1321 ggcgtcgacc acgctgctcg ggcgcgcgct ggtgcggcgg ttcgacggcg actcggcggc
     1381 gctgctccgc gcggtgctgg agatccacgc ccggccggtc gggcgcaggg agctgttcgc
     1441 cgagctggcg cggcgggcgg ggacggcgcc agacgagctg ccggcgcagc cgatcgggca
     1501 cacgtcgtgg ttgggcccgg tggcacaggt cggcggcgtg cagccgttgc tgcccacgcc
     1561 gc
//
LOCUS       DASVOR010001869         2769 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_428011,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001869 DASVOR010000000
VERSION     DASVOR010001869.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428011"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..1674
                     /locus_tag="VF516_34710"
     CDS             226..1674
                     /locus_tag="VF516_34710"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8112952.1"
                     /translation="MVAASTSSAQSGKPLLHICYAPADHAWVHGRMVRELGLEAGQYH
                     TRADDGLGELQLAAIERAVDECRFTVLVASTSARWNKLARFAADLAQHRGLEDQAPRL
                     IIVARDFALDAAAAQTQLSLGQRALVGLDCSDEDRTVESMARLRRLLALKPPVDEPPA
                     CPYPGLARFTAANRDLLFGRDGDRDALLQRIRAGHTRILVVGPSGTGKSSLVHAAVLP
                     ELSPEAHLAQVVPRGGDLAAALRTTIDALEVPDLGAAFDEYLAVTDDVTDAQIEAARA
                     RLCAVPAPDLRRRVVVVDPLEEIFAQDDAASRARLFQLLGGLWSLPWCTVILCMRADF
                     YGAVMVERCWRELESYQYPVVPLDEAGLRAAIAEPARHAGVHVDGALVERLIREVDRD
                     RSSAALPLLQVALKELWTHLRWRYLTLADYERIVSHDPARPGCRARGARRGGSATVDR
                     PDRRWARARRCARWCAAAWPRSDFAGDSSVPD"
     gene            1629..2015
                     /locus_tag="VF516_34715"
     CDS             1629..2015
                     /locus_tag="VF516_34715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112953.1"
                     /translation="MAAQRFRRRLFGAGLIAATAIAVVFVFLFLQLREEKTKTQASIR
                     EAVKVANQNVFELENNLRTVGCAEAAQVRDGLLRRTNDLLTELNKLSPLGEDEQRTGM
                     VAKQAQATLHWSVVGSPKRRSSIARR"
     gene            2012..2560
                     /locus_tag="VF516_34720"
     CDS             2012..2560
                     /locus_tag="VF516_34720"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8112954.1"
                     /translation="MTDEQRRAAKDPSNTDWQRDLSVSYNKLGDVAMFAGKLDDARAF
                     FDQALAVAKVLAAKDPSNTDWQRDLSVSYNKLGDVAVSAGKLDDARAFFDQALAVAKA
                     LAAKDPSNPVWQLDLCRTLAQRSVVSLRTHLPNEATRYLDEARAIYRRLQQAGLFQHD
                     VRFAQTGGVLNQLTAVAAASKR"
BASE COUNT          419 a          988 c          950 g          412 t
ORIGIN      
        1 cagctcagca ggaggcgcgg tcagagctcc gatgaaccac cggctcggtc agaccacgga
       61 gctgtccacc atcggggtgg gttcggtcgg cctggtgaac aatgttgcct aaccgcgggc
      121 agcctcttga gccgcggccg cgcgctacgg cttgactccg cggctcctgc ggaagagccg
      181 ccggctttgt tggaactagc cgagctgtgg cgcctactcg cagcgatggt cgccgcctcg
      241 acatcctcgg cccagtccgg caagccgctg ttgcacatct gctacgcacc tgccgaccac
      301 gcctgggtcc atggccggat ggtccgcgag ctcggcctgg aggctggcca gtaccacacc
      361 cgcgccgacg atggcctggg agagctgcaa cttgcggcga tcgagcgcgc ggtcgacgag
      421 tgccggttca ccgtgctcgt cgcctcgacc tcggcgcgct ggaacaagct ggcccggttc
      481 gccgccgacc tcgcgcagca tcgcgggctg gaggaccagg cgccacgcct gatcatcgtc
      541 gcgcgcgact tcgcgctcga cgcggcggcc gcgcagaccc agctctcgct cggtcagcgc
      601 gcgctggtgg gactcgactg cagcgacgag gatcgcaccg tcgagtcgat ggcccggctc
      661 cgcaggctcc tggcgctgaa gccgccggtc gacgagccgc cggcatgccc gtatcccggg
      721 ctcgcccggt tcaccgccgc caatcgcgac ctgctgttcg gccgcgatgg tgaccgggac
      781 gcactgctcc agcgcatccg cgccggccac acccgcatcc tggtcgtggg tccctcgggg
      841 acgggcaagt cgtcgctcgt ccacgccgcg gtgctgccgg agctgtcgcc cgaggcccac
      901 ctggcgcagg tcgtgccgcg cggcggggac ctggccgccg cgctccgcac caccatcgac
      961 gcgctggagg tgcccgacct gggcgccgca ttcgacgagt acctggccgt caccgacgac
     1021 gtcacggatg cgcagatcga agccgcccgc gcccgcctgt gcgccgtccc ggcacctgac
     1081 ctgcgccgcc gcgtcgtggt cgtcgatccg ctggaggaga tctttgcgca ggacgatgcc
     1141 gcctcccgcg cgcgactgtt ccagctgctc ggcgggctgt ggagcctgcc gtggtgcacc
     1201 gtgatcctgt gcatgcgagc ggacttctac ggggcggtga tggtcgagcg ctgctggcgc
     1261 gagctcgaga gctaccagta cccggtggtg ccactcgacg aggccgggct acgcgccgcg
     1321 atcgccgagc ccgcccggca cgccggcgtg cacgtcgacg gcgccctggt cgagcggctg
     1381 atccgcgagg tcgatcggga tcggtcgtcg gcggcgctgc cgctgctcca ggtcgcgctc
     1441 aaggagctct ggacccacct gcgctggcgc tacctgacgc tggcagacta cgagcggatc
     1501 gtgagtcacg acccagcgcg gcctggctgc cgtgctcgcg gtgcacgccg aggcggttct
     1561 gcaaccgttg accggccgga caggcgctgg gcgcgagcga ggcgctgcgc acgctggtgc
     1621 gccgcagcgt ggccgcgcag cgatttcgcc ggcgactctt cggtgccgga ctgatcgcgg
     1681 ccacggccat cgcggtggtc ttcgtgttcc tgttcctcca gctgcgggaa gaaaagacca
     1741 agacccaggc gtcgattcgc gaggcggtga aggtggcaaa ccagaacgtg ttcgagctgg
     1801 agaacaatct ccggactgtg ggctgcgccg aggccgcgca ggtgcgcgat ggcttgctga
     1861 ggcggacgaa cgatctcctc acggagctaa acaagctcag cccgctgggc gaggacgagc
     1921 agcggaccgg aatggtggcg aagcaggcgc aggcgacttt gcactggagc gtggtcggct
     1981 cgccgaagcg gagaagctct atcgcgaggc gctgaccgac gaacagcgac gcgccgccaa
     2041 ggaccccagc aacaccgact ggcaacgcga cctctcggtc tcctacaaca agctgggcga
     2101 cgtcgcgatg ttcgccggca agctcgacga cgcacgcgcc ttcttcgacc aggccctcgc
     2161 ggtggccaag gtgctcgccg ccaaggaccc cagcaacacc gactggcaac gcgacctctc
     2221 ggtctcctac aacaagctgg gcgacgtcgc ggtgtccgcc ggcaagctcg acgacgcacg
     2281 cgccttcttt gaccaggccc tcgccgtggc caaggcgctt gctgccaagg accccagcaa
     2341 cccggtttgg cagcttgacc tgtgccggac cctcgcccag cgttcggtgg tgtcgctgag
     2401 gacgcacctt cccaacgagg cgacgcgata cctcgacgag gcacgcgcca tctatcgcag
     2461 actgcagcaa gctggcctct tccagcacga tgtccggttc gcgcagaccg gcggagtgct
     2521 gaaccaactg accgcggtcg ccgccgcgtc gaagcgctga ttcatcacga ggttcgtggc
     2581 gcaggctgca cggctatggc gccgcggccc gcagcgggtc gcgagaggac aagcccccgc
     2641 gccgcgatct cccggcgcgc tcaatcttgc gccatcagcg ccttcaggaa cgcgtagtgg
     2701 aagtcgaccc cccggtagaa ggactccacc ggggtgcgct cgtccttgcc atgcgcgcga
     2761 agatcgtcg
//
LOCUS       DASVOR010001870         2421 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_195193,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001870 DASVOR010000000
VERSION     DASVOR010001870.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195193"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..588)
                     /locus_tag="VF516_34725"
     CDS             complement(<1..588)
                     /locus_tag="VF516_34725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8112955.1"
                     /translation="MQPDVTVVGAGMAGSEAAWQLAEAGLSVALIEMKPCAMSPVHQS
                     PLCGELVCSNSLRSDDPVAPAGLLKHELRRCGSLVIGCADQHRVPAGQALAVERFGFA
                     RAITRRLALHPRIRIERRRLDELPDGPVIVCTGPLTEGALGEVIRAELGGDRMYFYDA
                     IAPIVAADSIDWDFAFRGSRWGRDTAVDPGRRARPG"
     gene            complement(642..788)
                     /locus_tag="VF516_34730"
     CDS             complement(642..788)
                     /locus_tag="VF516_34730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112956.1"
                     /translation="MISAGATLLIYVGLYIAGVVEARLDLSRRGLWDQPAQPLPRARV
                     LRQR"
     gene            complement(867..>2421)
                     /gene="topA"
                     /locus_tag="VF516_34735"
     CDS             complement(867..>2421)
                     /gene="topA"
                     /locus_tag="VF516_34735"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEX8112957.1"
                     /translation="ERRKKPQAPFITSKLQQDAARKLRFSAKRTMALAQRLYEGVELG
                     DEGPTGLITYMRTDSTRVSDDALAAVRTHVTEVYGKDYLPDEPNQYGNKERAQDAHEA
                     IRPTQLEWTPDRVATALADHPEGPELIKLYTLIWQRFVASQMVPAVYDATTVDLDRGQ
                     AVLRATGQVMKFAGYTKVYEVAETDDAKAEAAEAADRLLPPLEIGHVIKLESIRPEQH
                     FTQPPPRFSEASLVKELEERGIGRPSTYASIMSTIVDRGYVEKRDARFFPTELGILVN
                     GLLVESFPEIVNSDFTAKMESDLDKVEEGAVDWRKLLGGFYTPFKDELEKARNEMRDV
                     KREEIATEWTCEKCGKPMVIKWGRNGSFLACQGYPECRNTQEVVKNLDGTWEKVPPQT
                     TDEVCEVCGAPMTVKRGRFGSFLACTKYPECKTTKPISLGVKCPRPGCGGFIAEKRSR
                     RGKPFYGCSNWAKKQCDFVAWDKPVPQPCPICNAKFVVKKENKRGVMLRCLECDWKQG
                     ADEAEDNAA"
BASE COUNT          326 a          885 c          817 g          393 t
ORIGIN      
        1 ccccggccgg gcgcgcctgc cggggtcgac ggcggtgtcg cggccccagc gcgagccgcg
       61 gaacgcgaag tcccagtcga tcgagtcggc cgcgacgatc ggcgcgatcg cgtcgtagaa
      121 gtacatgcga tcgccgccga gctcggcgcg gatcacctcg cccagcgcgc cctcggtcag
      181 cggccccgtg cagacgatca ccggtccgtc gggcagctcg tcgagccggc gccgctcgat
      241 ccggatccgc ggatggagcg cgagccgccg ggtgatcgcg cgcgcgaacc cgaagcgctc
      301 gaccgcgagc gcctggccgg cgggcacgcg gtgctggtcg gcgcagccga tcaccagcga
      361 gccgcagcgc cggagctcgt gcttgagcag cccggccggc gcgaccggat cgtcgctgcg
      421 cagcgagttg ctgcacacca gctcgccgca cagcggggac tggtgcaccg gcgacatcgc
      481 gcacggcttc atctcgatca gggcgaccga caggccggcc tcggcgagct gccaggccgc
      541 ctcgctgccg gccatgccgg ccccgaccac ggtgacgtcc ggctgcatcg ccggaaagct
      601 accaccgccg gcggtgcatc gcctcgccgg ccccgcgctc gctatcgctg ccgcaacacc
      661 cgcgcccggg gcagcggctg cgccggctgg tcccacagtc cgcgccgcga cagatcgagg
      721 cgcgcctcga ccacgccggc gatgtacagg ccgacgtaga tgagcagcgt cgcgcccgcg
      781 ctgatcacgc catacatgtt ccctcccggt gaccttgggt cacctcccca gtatgacacg
      841 cccggggcgg ccggcgtcat gcaacgtcag gcggcgttat cctcggcctc gtcggcacct
      901 tgcttccagt cacactccag gcaccgcagc attacgccgc gcttgttctc cttcttgacc
      961 acgaacttcg cgttgcagat cgggcacggc tgtgggaccg gcttgtccca cgccacgaag
     1021 tcgcactgct tcttggccca gttggagcag ccgtagaacg gcttgccgcg ccgcgaccgc
     1081 ttctcggcga tgaacccacc gcagcccggc cgcgggcact tgaccccgag cgagatcggc
     1141 ttggtggtct tgcactccgg gtacttggtg catgccagga acgacccgaa ccggccgcgc
     1201 ttgaccgtca tgggcgcgcc gcacacctcg cacacctcat cggtggtctg cggcggtact
     1261 ttctcccacg tgccgtcgag gttcttgacc acctcctgcg tgttgcggca ctcggggtag
     1321 ccctggcagg ccaggaacga gccattgcgg ccccacttga tcaccatcgg cttgccgcac
     1381 ttctcgcacg tccactcggt ggcgatctcc tcgcgcttga cgtcgcgcat ctcgttgcgc
     1441 gccttctcga gctcgtcctt gaacggcgtg tagaacccgc cgagcagctt gcgccagtcg
     1501 accgcgccct cctcgacctt gtcgagatcg gactccatct tggcggtgaa gtccgagttc
     1561 acgatctcgg gaaagctctc gaccagcagg ccgttgacca ggatgccgag ctcggtcggg
     1621 aagaaccgcg cgtcgcgctt ctcgacgtag ccgcggtcga cgatggtcga catgatcgac
     1681 gcgtaggtcg acggccggcc gatgccgcgc tcctcgagct ccttgaccag cgacgcctcg
     1741 gagaaccgcg gcggcggctg ggtgaagtgc tgctcgggcc ggatcgactc gagcttgatc
     1801 acgtggccga tctcgagcgg cggcagcagg cggtcggccg cctcggcggc ctcggccttg
     1861 gcgtcgtcgg tctcggcgac ctcgtagacc ttggtgtagc cggcgaactt catgacctgg
     1921 ccggtcgcgc gcagcaccgc ctggccgcgg tcgaggtcca ccgtggtggc gtcgtacacc
     1981 gccggcacca tctgcgacgc gacgaaccgc tgccagatca gcgtgtacag cttgatcagc
     2041 tcggggccct cggggtggtc ggcgagcgcg gtggcgaccc ggtccggcgt ccactccagc
     2101 tgggtcggcc ggatcgcctc gtgcgcgtcc tgcgcgcgct ccttgttgcc gtactggttg
     2161 ggctcgtccg gcaagtagtc cttgccgtag acctcggtga cgtgggtgcg caccgcggcc
     2221 agcgcgtcgt cggacacgcg ggtcgagtcg gtacgcatgt acgtgatgag gccggtcggt
     2281 ccctcgtcgc cgagctcgac gccctcgtac agccgctggg cgagcgccat cgtccgcttg
     2341 gcggagaacc gcagcttgcg cgcggcgtcc tgctgcagct tgctggtgat gaacggcgcc
     2401 tgcggcttct tgcgccgctc c
//
LOCUS       DASVOR010001871         2245 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340594,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001871 DASVOR010000000
VERSION     DASVOR010001871.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340594"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VF516_34740"
     CDS             complement(<1..694)
                     /locus_tag="VF516_34740"
                     /inference="COORDINATES: protein motif:HMM:NF016627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003839 - gamma-glutamylcyclotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0061928 - glutathione specific
                     gamma-glutamylcyclotransferase activity [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HEX8112958.1"
                     /translation="MTAPGDPVWVFGYGSLIWRPAMAHVARRASRIDGWARRFWQAST
                     DHRGTVEAPGRVLTLVESDGAVWGMAYAIARAAWPETEAALELREQQGYARLTVDIGL
                     AAGERAGPVVETVAGLLYVATPANPYFIGPEPLAATADVVLRSHGPSGSNVDYVLALD
                     HMLAAMGAADPEVSALAEHHRAAQRRPTRGGRPRASAPAWAARWPDGSRGRRRSAPPA
                     ARAGSTRHRGTST"
     gene            complement(750..1988)
                     /locus_tag="VF516_34745"
     CDS             complement(750..1988)
                     /locus_tag="VF516_34745"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8112959.1"
                     /translation="MAAPAPPKAFSALGLPGYRAYLATLTLTMMADNVEHVISYWVAF
                     RQFHSAALGGFAVLSHWVPYIALAVAVGGLNDRFDSRRLIQAGGLLFMFVSIAWGYLF
                     ATHSLQIWHAMVLLALHGCASLLWVVSGQVLLYDIVGPEALPSAVRLSATGRYLGLLI
                     GPGVGSVMMLTLGPTLGIFCNALFYLPLVIWLVRAPYGRQFRGDRPPQRRAVRGLADI
                     VATIREVRSIPLLATMIALAGSASFFLASGYQAQMPSFAQDLGHSDPGTSYAMLLGAD
                     ATGALVGGILLESSGGVFRTRPRTAMILAIAWALSLGGFASTQVYPLALVLLFLAGFF
                     ELSFSSMTQTLVQIHAPNASRGRVLGLYNMASMGLRAFSGVTVGLAGSIVGIHVSLAA
                     AAGLLIVVILALLVRARRIA"
     gene            complement(2016..>2245)
                     /locus_tag="VF516_34750"
     CDS             complement(2016..>2245)
                     /locus_tag="VF516_34750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112960.1"
                     /translation="TEPPCALTRGDLPLTRPVMGAISPPPPEIHQPAQIEAPQPPPRA
                     TMGLPSWHVPPSQPEARPTRRPAPAAGKRAR"
BASE COUNT          330 a          774 c          882 g          259 t
ORIGIN      
        1 cggtcgaggt gccgcgatgt cgcgtcgaac cggcgcgcgc ggccggcggg gctgagcgcc
       61 gacgcccgcg cgatccatcg ggccatcgcg ctgcccaggc cggcgcgctc gcgcggggcc
      121 gtccgccacg ggtcggacgg cgctgcgccg cccggtggtg ctcggcgagc gcgctcacct
      181 cgggatccgc cgcgcccatc gcggccagca tgtggtcgag cgcgagcacg tagtcgacgt
      241 tgctgccgct cgggccgtgg gatcgcagga cgacgtcggc ggtcgccgcg agcggctcgg
      301 gtccgatgaa gtacgggttg gcgggggtcg cgacgtacag cagcccggcg acggtctcga
      361 ccacggggcc ggcccgctct ccggcggcca ggccgatgtc cacggtcagg cgggcgtagc
      421 cctgctgctc gcggagctcg agggcagcct cggtctccgg ccacgcggcg cgcgcgatcg
      481 cgtaggccat gccccacacc gcgccgtcgc tctcgacgag cgtgagcacg cggccgggcg
      541 cctcgacggt gccgcgatga tcggtcgacg cttgccagaa ccggcgcgcc cagccgtcga
      601 tgcggctggc gcgacgcgcc acgtgcgcca tcgccggccg ccagatcagg gacccgtagc
      661 cgaacaccca gaccgggtcc ccgggcgcgg tcaccgcttg cctccggcga tccgtcgggc
      721 cgcgtgcggc gcggtcgctg cgcgacgcgt catgcgatgc ggcgcgcgcg gaccagcagc
      781 gcgaggatca ccacgatcag caggcctgcc gcggcggcga gcgagacgtg gatgccgacg
      841 atgctgccgg cgaggccgac cgtgacgccg ctgaacgctc gcagtcccat cgatgccatg
      901 ttgtagaggc cgagcacccg gccgcggctg gcgttcggcg cgtggatctg caccagcgtc
      961 tgggtcatgc tgctgaacga cagctcgaag aacccggcga ggaacagcag gacgagggcg
     1021 agcgggtaga cctgggtgct ggcgaacccg ccgagcgaga gcgcccaggc gatcgcgagg
     1081 atcatcgcgg tgcgcggccg cgtccggaac acgccgccgc tgctctcgag caggatgccg
     1141 ccgaccaggg cgccggtggc gtcggcgccg agcagcatgg cgtaggaggt cccggggtcg
     1201 ctgtgcccga ggtcctgggc gaagctgggc atctgcgcct ggtagccgct ggcgaggaag
     1261 aacgaggcgc tgccggcgag ggcgatcatc gtcgccagga gcgggatcga ccggacctcg
     1321 cggatcgtcg cgacgatgtc ggcgaggccg cggacggcgc ggcgctgcgg cgggcggtcg
     1381 ccgcggaact gccggccgta aggcgcgcgg accagccaga tcacgagcgg caggtagaac
     1441 agcgcgttgc agaagatgcc gagcgtcggg cccagcgtca gcatcatgac gctgccgacg
     1501 ccggggccga tgagcaggcc gaggtagcgc cctgtcgcgg acagccgcac cgcgctgggc
     1561 agcgcctcgg gaccgacgat gtcgtagagc agcacctggc ccgacaccac ccacaagagg
     1621 ctggcgcagc cgtgcagggc gagcaggacc atcgcgtgcc agatctggag cgagtgggtc
     1681 gcgaacaggt agccccacgc gatcgacacg aacatgaaca gcaggccgcc ggcctggatc
     1741 aggcgccgtg agtcgaagcg gtcgttgagg ccgccgacgg cgaccgcgag agcgatgtag
     1801 ggcacccagt gcgacaggac ggcaaagccg ccgagcgccg ccgagtggaa ctggcggaac
     1861 gcgacccagt agctgatgac gtgctcgacg ttgtcggcca tcatcgtgag cgtcagcgtc
     1921 gccaggtacg cgcggtagcc cggcaggccg agggcgctga acgctttcgg aggggcaggg
     1981 gcggccacgg cccccgcacc ataaaacatc cgtggtcagc gcgctcgctt gcccgcggcc
     2041 ggcgccggcc gtctggtcgg gcgagcctcc ggctgtgagg gtgggacgtg ccaggacggc
     2101 agccccatcg tcgcccgggg gggcggctgc ggagcctcga tctgcgcggg ctggtggatc
     2161 tccggcgggg gcggcgagat cgcgcccatc accgggcggg tcagaggcag atcgccgcgc
     2221 gtcagtgcgc acggcggctc ggtcg
//
LOCUS       DASVOR010001872         2846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_515988,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001872 DASVOR010000000
VERSION     DASVOR010001872.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_515988"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VF516_34755"
     CDS             <1..183
                     /locus_tag="VF516_34755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112961.1"
                     /translation="YIDRRAAALLDAARPSSTIVRPHDVEIAFDGALADRDRIGAAIE
                     LAAHWACDARAMGPYR"
     gene            266..>2846
                     /gene="gyrA"
                     /locus_tag="VF516_34760"
     CDS             266..>2846
                     /gene="gyrA"
                     /locus_tag="VF516_34760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEX8112962.1"
                     /translation="MSDVTAPNITPISIEAEMTSSFMDYAMSVIVSRALPDARDGLKP
                     VHRRILFAQRGLNNTYNRPYLKCARIVGDVIGKYHPHGDSAVYDALVRMAQDFSMRYM
                     LVDGQGNFGSVDGDPPAAMRYTECRMSKLAGELLADIDKETVDWQPNYDDKELEPTVL
                     PTKVPNLLINGSSGIAVGMATNIPPHNLGEIIDGVLALIADPRLPDDELVKLIPGPDF
                     PTGGVIQGRVGILSAYRTGRGTVVVRGVAHSEEIRKDRTAIVVTELPFMVNKSRWIEA
                     TAELVREKKLEGISDIRDESDRTGMRVVIELKRDADDKVVVNNLFKHTQLQTSFAINM
                     LAIVEGKPVLLSLRRALQVFVDHRRDVVTRRTLFDLREARARREIVEGLGLAVLNVDR
                     VIDIIRSSKDPDEAKARLIDEPMSGLAGFLERAGRPAEEVAAASARGTVTLTARQAQA
                     ILDMRLGRLTGLEREKLEGEYKELWQLTDYLESLLADEAKLMAAIIDELRQIKTEFAD
                     PRRTQIVDAEGEILTEELIDQEDMVVTRTHLGYIKRTPVDEYEAQGRGGRGITGATSM
                     EGDFIVDMFAASTHDHLLLFTDKGRAYYKKVFELPEGSRTAKGRAIVNVLDLQEGEQV
                     VAMLPFKEFRADTCVFFATQSGTVKKTELSQFENVRQSGIKAITIEDGDRLIKAELTT
                     KAHDVLLTSAKGFAVRFKEDRVRPMGRTAAGVRGISLRADDRLVGMCTFMRDDSAATL
                     ITVCERGYGKRTALADYPTKNRGGKGVITIKTTTRNGLVAATRIVTDDDHLILISDRG
                     KLIRLRVGEIKVQGRATQGVRLMRVDDGEHVAAIERLAEPGETSGIAEGAPIEAVDDN
                     DTVP"
BASE COUNT          494 a          997 c          974 g          381 t
ORIGIN      
        1 tacatcgatc ggcgcgccgc ggcgctgctc gacgccgcgc ggccgagctc gacgatcgtc
       61 cggccgcacg acgtcgagat cgcgttcgac ggcgcgctcg ccgatcgcga ccggatcggc
      121 gcggcgatcg agctcgcggc gcactgggcg tgcgacgctc gcgcgatggg cccctatcgc
      181 tagcgatctc gtcggaaaac cgtggttttt tgggcgtccc ggcgcgttga gaaccgggcg
      241 tccgcaggct agaaatgccc tcgcgatgtc ggacgtaacc gcccccaata tcacgccgat
      301 ctccatcgag gcggagatga cgagctcgtt catggactac gccatgagcg tgatcgtgtc
      361 gcgggccctg cccgacgccc gcgacggcct caagccggtc caccgccgga tcctgttcgc
      421 ccagcggggg ctcaacaaca cgtacaaccg gccgtacctc aagtgcgcgc gcatcgtcgg
      481 cgacgtgatc ggcaagtacc acccgcacgg cgacagcgcg gtctacgacg ccctggtccg
      541 catggcccag gacttctcga tgcgctacat gctggtcgac ggccagggca acttcggctc
      601 ggtcgacggc gatccgccgg cggcgatgcg ttacaccgag tgccggatga gcaagctcgc
      661 cggcgagctg ctcgcggaca tcgacaagga gaccgtcgac tggcagccga actacgatga
      721 caaggagctc gagcccacgg tcctgccgac caaggtcccg aacctcctga tcaacggctc
      781 gtcgggtatc gcggtcggca tggcgaccaa catcccgccg cacaacctgg gcgagatcat
      841 cgacggcgtg ctcgcgctga tcgccgaccc gcggctgccc gacgacgagc tggtcaagct
      901 gatccccggc cccgacttcc cgaccggcgg cgtgatccag ggccgggtcg gcatcctgtc
      961 ggcgtaccgg accggccgcg gcaccgtggt cgtgcgcggc gtcgcccaca gcgaggagat
     1021 ccgcaaggac cgcacggcga tcgtggtcac cgagctgccg ttcatggtca acaagtcgcg
     1081 ctggatcgag gcgaccgccg agctggtccg cgagaagaag ctcgagggca tcagcgacat
     1141 ccgcgacgag tccgaccgca ccgggatgcg ggtcgtgatc gagctcaagc gcgacgccga
     1201 cgacaaggtc gtggtcaaca acctgttcaa gcacacccag ctccagacct cgttcgccat
     1261 caacatgctg gcgatcgtcg agggcaagcc ggtgctgctg tcgctgcgcc gcgcgctgca
     1321 ggtgttcgtc gaccatcgcc gcgacgtggt cacccgccgc acgctgttcg atctgcgcga
     1381 ggcccgcgcg cgccgcgaga tcgtcgaggg cctcggcctg gcggtgctca acgtcgaccg
     1441 ggtgatcgac atcatccggt cgagcaagga ccccgacgag gccaaggccc ggctgatcga
     1501 cgagccgatg tccggcctcg ccggcttcct cgagcgcgcc gggcggccgg ccgaggaggt
     1561 cgcggcggcg agcgcgcgcg gcaccgtcac gctgaccgcg cgccaggccc aggcgatcct
     1621 cgacatgcgg ctcggccggt tgaccggcct ggagcgcgag aagctcgagg gcgagtacaa
     1681 ggagctgtgg cagctcaccg actacctcga gagcctgctc gccgacgagg ccaagctgat
     1741 ggcggcgatc atcgacgagc tgcgccagat caagaccgag ttcgccgacc cgcgccgcac
     1801 ccagatcgtc gacgccgagg gcgagatcct caccgaggag ctgatcgacc aggaagacat
     1861 ggtcgtgacc cgcacccacc tcggctacat caagcggacg ccggtcgatg agtacgaggc
     1921 ccagggccgg ggcggccgcg gcatcaccgg cgcgacgtcg atggagggcg acttcatcgt
     1981 cgacatgttc gcggcgtcga cccacgacca cctgctgctg ttcaccgaca agggccgcgc
     2041 ctactacaag aaggtgttcg agctgcccga gggctcgcgc accgccaagg gccgcgcgat
     2101 cgtcaacgtg ctcgacctcc aggagggcga gcaggtggtc gcgatgctgc cgttcaagga
     2161 gttccgcgcc gacacctgcg tgttcttcgc gacccagtcc ggcacggtca agaagaccga
     2221 gctgtcgcag ttcgagaacg tccgccagag cgggatcaag gcgatcacga tcgaggacgg
     2281 cgaccggctg atcaaggccg agctcacgac caaggcccac gacgtgctcc tgacctcggc
     2341 caagggcttc gcggtgcggt tcaaggagga tcgcgtgcgg ccgatgggac ggaccgcggc
     2401 gggcgtgcgc ggcatctcgc tgcgcgccga cgaccgcctg gtcgggatgt gcacgttcat
     2461 gcgcgacgac tcggcggcca cgctgatcac cgtgtgcgag cgcggctacg gcaagcgcac
     2521 cgcgctcgcc gactacccga ccaagaaccg cggcggcaag ggcgtcatca cgatcaagac
     2581 caccacgcgc aacggcctgg tcgcggcgac ccggatcgtc accgacgacg accacctgat
     2641 cctgatctcc gaccgcggca agctgatccg gctccgggtc ggcgagatca aggtccaggg
     2701 ccgcgcgacc cagggcgtcc ggctgatgcg ggtcgacgac ggcgagcacg tcgcggcgat
     2761 cgagcggctc gccgagccgg gcgagacctc ggggatcgcc gagggcgcgc cgatcgaggc
     2821 ggtcgacgac aacgacacgg tgccca
//
LOCUS       DASVOR010001873         2480 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_195230,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001873 DASVOR010000000
VERSION     DASVOR010001873.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195230"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF516_34765"
     CDS             <1..447
                     /locus_tag="VF516_34765"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112963.1"
                     /translation="IVAGDGFTCAHTVPDGVWCWGFGFNGEIGIDDFEPFTTPQHLTA
                     LTGTMKIIAGGSHACAIKASGALACWGASYNGEIGDGGYGNRGTPVAVAMPGNASVLD
                     VSAGEQHTCAVLADGSVACWGDDRFGQLGDGILADQTPVAPLLPCP"
     gene            510..2210
                     /locus_tag="VF516_34770"
     CDS             510..2210
                     /locus_tag="VF516_34770"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8112964.1"
                     /translation="MRRLYLLAILALVSCASAPARSTGERYDLLIKNGVVYDGSGAAP
                     QRVDVAINGDRVVAWLPSGGRAEARRVVDAQGQAVAPGFINVLSWSTESLIADGRGVS
                     DTRQGVTLEIFGEGWSMGPLDPAMKALALKRQTDIKYPIEWTTLGEYLDYLAHRGITP
                     NIASFVGATTVRIHELGEADVQPDAAQLARMQNLVRAAMREGALGVGASLIYPPAFYA
                     RSGELTALAKAAAELGGGYIAHMRSEANGLLEAIDETLAIGRATGQRVEAYHLKAAGE
                     RNWAKMAQAIGKIEAARAAGIQASANMYTYTAGATGLSASLPPWVQDGGHEAMIGRLK
                     DPAQRARILAEMKVPSNAWENLRLLAGSADRVLLLGFKNDQLKPLTGKNLAEVAARRG
                     TPVEDTILDLIIEDDSRVETAYFLMSEDNVELGLQQPWVSLGSDAESSAPEGVFLQSS
                     THPRAYGNFSRFLGHYARDRKLMPLEQAIHRLTGLPAHNWKLDSRGCLDAGCYADVVV
                     FDPLTIVDHATYDRPQQFATGVSEVFVNGVLVLHAGEHTGATPGRVVRGPGWTGGRRN
                     "
     gene            complement(2243..>2480)
                     /locus_tag="VF516_34775"
     CDS             complement(2243..>2480)
                     /locus_tag="VF516_34775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112965.1"
                     /translation="ERVDEPVDDLGRQGVTLNGTPIKPGAFKTGLTLPLLDQGQDPTR
                     ATWKLVPAGGADKFSARNLRTIALVVTYATRPSF"
BASE COUNT          371 a          890 c          877 g          342 t
ORIGIN      
        1 atcgtcgcgg gcgacggctt cacctgcgcg cacaccgtgc ccgatggcgt gtggtgctgg
       61 ggcttcggat tcaacggcga gatcgggatc gacgacttcg aacccttcac cacgccgcag
      121 cacctgaccg cgctgaccgg cacgatgaag atcatcgccg gcggctcgca tgcctgcgcg
      181 atcaaggcca gcggcgcgct cgcgtgctgg ggcgcgagct acaacggcga gatcggcgac
      241 ggcggctacg gcaaccgcgg cacgccggtc gcggtggcga tgcccggcaa cgccagcgta
      301 ctcgacgtct ccgccggcga gcagcacacc tgcgccgtgc tcgccgacgg cagcgtcgcg
      361 tgctggggcg acgaccgctt cggccagctc ggcgacggca tcctcgccga tcagacgccg
      421 gtcgcgccgc tcctgccgtg cccgtgaccg gccggcgcgc agtcgtctaa gccgtgcggg
      481 ctgcgttatg ctggcgcgca ggagagccca tgcgacgtct ctacttgctt gcgatcctgg
      541 cgctggtgag ctgcgcgtcc gcgccggcgc gctcgacggg cgagcgctat gatctgctga
      601 tcaagaacgg cgtcgtgtac gacgggagcg gcgccgcgcc ccagcgcgtc gacgtggcga
      661 tcaatggcga ccgcgtcgtc gcctggctgc cgagcggcgg gcgggcggag gctcgccggg
      721 tcgtcgacgc gcagggccag gcggtcgcac cgggcttcat caacgtgctc agctggtcga
      781 cggagtcgct gatcgccgac ggccgcggtg tcagcgacac caggcagggc gtgacgctgg
      841 agatcttcgg cgagggctgg tcgatggggc cgctcgatcc ggcgatgaag gcgctcgcgc
      901 tcaagcggca aaccgacatc aagtacccga tcgagtggac cacgctcggc gagtatctcg
      961 actacctcgc ccaccgcggc atcacgccca acatcgcgtc gttcgtcggc gcgaccaccg
     1021 tgcgcatcca cgagctcggc gaggccgacg tccagcccga cgccgcgcag ctcgcgcgga
     1081 tgcagaacct cgtgcgcgcg gcgatgcgcg agggcgcgct cggcgtcggc gcctcgctga
     1141 tctacccgcc ggcgttctac gccaggtccg gcgagctgac cgcgctggcg aaggccgccg
     1201 ccgagctcgg cggcggctac atcgcccaca tgcggagcga ggcgaacggt ctgctcgagg
     1261 cgatcgacga gaccctcgcg atcggccgcg ccaccgggca gcgcgtcgag gcctatcacc
     1321 tcaaggccgc gggcgagcgc aactgggcca agatggcgca ggcgatcggc aagatcgagg
     1381 ccgcgcgcgc cgccggcatc caggcaagcg ccaacatgta cacctacacc gccggtgcga
     1441 cgggactgtc ggcgagcctt ccgccgtggg tccaggacgg cggacacgag gcgatgatcg
     1501 gtcggctcaa ggatccggcg cagcgcgcgc ggatcctcgc cgagatgaag gtgcccagca
     1561 acgcgtggga gaacctgcgg ctgctcgccg gcagcgccga ccgcgtgctg ctgctcggct
     1621 tcaagaatga ccagctcaag ccgctcaccg gcaagaacct cgccgaggtc gcggcccggc
     1681 gcggcacacc ggtcgaggac accatcctcg atctgatcat cgaggacgac tcgcgcgtcg
     1741 agaccgccta cttcctgatg agcgaggaca acgtcgagct cggcctccag caaccgtggg
     1801 tcagcctcgg ctccgacgcc gagtcgtcgg cgcccgaggg cgtgttcctg cagtccagca
     1861 cgcatccacg cgcctacggc aacttcagcc gcttcctcgg ccactatgcg cgggaccgca
     1921 agctcatgcc gctcgagcag gcgatccatc gcctcaccgg cttgccggcg cacaactgga
     1981 agctcgacag ccgcggttgc ctcgacgccg gctgctacgc cgacgtcgtg gtgttcgacc
     2041 ctctgacgat cgtggaccac gccacgtacg atcgcccgca gcagttcgcc accggggtca
     2101 gcgaggtgtt cgtcaacggc gtgctcgtgc tgcacgccgg cgagcacacc ggcgcgacgc
     2161 ccgggcgggt cgtccgcgga ccgggctgga cgggcggccg gcgcaactga gcgcgcgggg
     2221 cggttcctcg cccgcgcctg gctcagaatg acggcctggt cgcgtaggtg acgaccagcg
     2281 cgatggtcct gaggttccgg gcgctgaact tgtccgcgcc accggccggc accagcttcc
     2341 acgtcgcgcg cgtcggatcc tggccctggt cgaggagcgg cagggtgagc ccggtcttga
     2401 acgcgcccgg cttgatcggg gtgccgttga gcgtgacccc ctggcggccg agatcgtcca
     2461 ccggctcgtc gacccgctcg
//
LOCUS       DASVOR010001874         5248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_165514,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001874 DASVOR010000000
VERSION     DASVOR010001874.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5248
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165514"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1226
                     /locus_tag="VF516_34780"
     CDS             <1..1226
                     /locus_tag="VF516_34780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112966.1"
                     /translation="GAADPPWASSSPRAPAGSGASGEGAPGAPLSIDSARRRDDGIEP
                     PPTRIQRPEALVDRPFAPPKVSPIPEIPEPGLFHSARYTVRFARARWQRRGAIKLLGA
                     DIKKDTDALDQVLGALGGVARTAKVDGRVFFGENAAINAAEERVAQLTREHNDVDARK
                     AEENSKFVDVERERNTKLAEAERMVDEAQKELAGLEAQRRGLRDKRKELERRQKAYLK
                     AAEDSDKQAGNVELGDARQEHRRSAEQNRREAAALEPERQDVDRRLTGIERPIGEATA
                     RLDAAKAELDAAKRSLADAREGHIHRLAELDAEQKRKGREIAQAEGEIARRLVTLGTL
                     VNLNRIEDPRFEELYQRIDRLRGAITARTTEIERLTAEREAYDRGNLVRGLAAIGGAI
                     LVFILLIAILRAVLR"
     gene            complement(1304..1720)
                     /locus_tag="VF516_34785"
     CDS             complement(1304..1720)
                     /locus_tag="VF516_34785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112967.1"
                     /translation="MIDIRNQVGRLIEVTIESPTTTSEWKAIPGQLTPIHGRLPGKYM
                     YCANISRARLLRPEEVEPMKKIMQLDNPRLLRTALYLGSNSATLMLQMERLIRETSNP
                     ERKTFRDPQKLIAWMSEVTTAEEQERISELYGNLSS"
     gene            complement(1774..2640)
                     /locus_tag="VF516_34790"
     CDS             complement(1774..2640)
                     /locus_tag="VF516_34790"
                     /inference="COORDINATES: protein motif:HMM:NF040195.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010333 - terpene synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0046246 - terpene biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terpene synthase family protein"
                     /protein_id="HEX8112968.1"
                     /translation="MTTLDAELHDLLAWGSELEGFTETHARDGLVLAHSYIEGIPDGD
                     GGWIAGIGAVHERCALLLATDLAWLLWLDDCFDLGEAPVDLDLLLAAIDGPAWSREGR
                     GYQHLRTQMQQATTCPRALAVWTQTASDVFRAYHRNAEWARAPRPLSYGEYLDNGEHS
                     IATTHFVATTALIYGWDLAARLRKDTFRRCLRHLSLLTRLQNDLASAERERHDRTPAN
                     AVLLLEPMIGPTGARELVRRELDGYRRSLHRDLTEIDRGDPFCGFVNVLVRATDQYYS
                     DPRARYQVTDPI"
     gene            complement(2644..3606)
                     /locus_tag="VF516_34795"
     CDS             complement(2644..3606)
                     /locus_tag="VF516_34795"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8112969.1"
                     /translation="MNSIQPTTETHERHASGPGASLKAKDPATPQAPELTERPQDCAG
                     VKAYYNHKTASILKKYGPGPRVHFHIGLYEKDPFRPGLDMEQLRTEITSAQERMVAHA
                     AEVWEADWALSGDILDVGCGLGGGSLYWAAEHGARVTSVTIASEHIELIQSFAAQAGV
                     ANRIETVLSDCCEVPTGRRYDGAVAMESACYFPRWKWFRHLAELIRPGGFVCIEDTFL
                     GSPEWKTPFDNYWHTDIAPVDEYVSAARAAGFVLDRAEDVTQLTTPFWLQSAAWSKRM
                     LASGKLSDLEAARLRTSIDWHHQFSRAWKEGAIDVQLLRFRHRG"
     gene            4064..5158
                     /locus_tag="VF516_34800"
     CDS             4064..5158
                     /locus_tag="VF516_34800"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8112970.1"
                     /translation="MPKVLVSNNSELLRHFTARPFQRLDLQLIVAGSAAEAHALFRQE
                     QPALAVIDAEPGGFDTARAIKADNPATRVVLVAGKQLSGDQMRQVSISGCDELLIAPM
                     TADELHDVVAIQLGEPRPGSEAFSVAVQLAGKPVDATVSNLSVDGVRLVVGEPVSEGQ
                     ALELSIAPEGDAPVEIRGTAVWAQPRDGKTVVGVAFDKPDDRARAVLARLTQWQVVKD
                     GERIRVVLRGDFTEATRFDELLPAMVGRVVFDTAQVTYMNSLGVRAWCEFLRQARIQG
                     YEFHACSVPFILQASMVRDVIGRGTVTSFFAPFHCIACDHQEERLLQSAAILASNLEP
                     PTFKCPRCGGALEFDDLPERYFAFLQDDPE"
BASE COUNT          815 a         1755 c         1852 g          826 t
ORIGIN      
        1 ccggcgccgc cgatccaccg tgggcgagca gctccccgcg cgcgcccgcc gggtccggag
       61 cgagcggcga gggcgcgccg ggagcaccgc tcagcatcga cagtgcacgc cgccgcgacg
      121 acggcatcga gccgccgccg acccggatcc agcggcccga ggcgctggtc gaccgcccgt
      181 tcgcgccgcc caaggtcagc ccgatccccg agatccccga gcccggcctg ttccactcgg
      241 cgcgctacac cgtgcggttc gcgcgggcgc gctggcagcg ccgcggcgcg atcaagctgc
      301 tcggcgccga catcaagaag gacaccgacg cgctcgatca ggtgctcggc gcgctcggcg
      361 gcgtcgcgcg caccgccaag gtcgatggtc gggtgttctt cggcgagaac gccgcgatca
      421 acgccgccga ggagcgcgtc gcgcagctga cccgcgagca caacgacgtc gacgcccgca
      481 aggccgagga gaactcgaag ttcgtcgacg tcgagcgcga gcgcaacacc aagctcgccg
      541 aggccgagcg catggtcgac gaggcccaga aggagctcgc gggcctcgag gcccagcgcc
      601 gcggcctgcg cgacaagcgc aaggagctcg agcgccgcca gaaggcctac ctcaaggccg
      661 ccgaggacag cgacaagcag gccggcaacg tcgagctcgg cgacgcgcgc caggagcacc
      721 ggcgcagcgc cgagcagaac cgccgcgagg ccgccgcgct ggagcccgag cgccaggacg
      781 tcgaccgccg gctcaccggg atcgagcggc cgatcggcga ggcgaccgcc cggctcgacg
      841 ccgccaaggc cgagctcgac gccgccaagc gcagcctggc cgacgcccgc gagggccaca
      901 tccaccggct cgccgagctc gacgccgagc agaagcgcaa gggccgcgag atcgcccagg
      961 ccgagggcga gatcgcccgc cggctggtca ccctcggaac cctggtcaac ctcaaccgga
     1021 tcgaggaccc gcggttcgag gagctctacc agcggatcga ccggctgcgc ggcgcgatca
     1081 cggcgcgcac caccgagatc gagcggctga ccgcggagcg ggaggcctac gaccgcggca
     1141 acctggtgcg cggtctggcc gcgatcggcg gggcgatcct cgtgttcatc ctgctgatcg
     1201 cgatcctgcg cgccgtcctg cgctgagccg ggaccgccac gcggtgccct ccggccagga
     1261 ggacggagcc ggacgggggc aggtgttcct ccgtgcgccg ctgtcaggag gagaggttgc
     1321 cgtagagctc ggagatccgc tcctgctcct cggcagtcgt gacctcggac atccaggcga
     1381 tgagcttctg gggatcgcgg aaggtcttgc gctccggatt ggaagtctcg cggatcaggc
     1441 gctccatctg tagcatcagg gtcgcgctgt tgctgccgag gtagagcgca gtgcggagca
     1501 gccgggggtt atccagctgc ataattttct tcatgggctc gacttcctcc ggccggagca
     1561 gccttgccct ggagatattt gcacaataca tgtattttcc tggcaatcgg ccatggatcg
     1621 gggtcagctg cccgggaatc gccttccact ccgatgtcgt ggtcggcgat tcgatggtaa
     1681 cctcgatgag ccgcccgact tggttcctga tatcgatcac tccgcgtctc cttgctcgcc
     1741 gatggcgacg gcgagtgccg atgagcacgg gagttatatc ggatctgtca cttggtagcg
     1801 cgctcgcggg tcactatagt actggtcagt cgcccgcacc aaaacattca cgaatccaca
     1861 aaacggatcg ccgcgatcga tctcggtgag atctcggtga agcgagcggc ggtacccgtc
     1921 gagctcgcgg cgcacgagct cgcgggcccc ggtcggaccg atcatcggct cgagcagcag
     1981 caccgcgttg gccggagtgc ggtcgtggcg ctcgcgttcg gcgctcgcga ggtcgttctg
     2041 caggcgagtc agcaagctga ggtgccgtag gcagcggcgg aacgtatcct tgcgcaggcg
     2101 ggcggcgagg tcccagccgt agatcagcgc ggtggtggcg acgaagtgcg tggtggcgat
     2161 gctgtgctcc ccgttgtcga ggtattcacc gtaggacagg ggccgcggcg cgcgcgccca
     2221 ctcggcgttg cggtggtagg cgcggaagac gtcgctcgcc gtctgggtcc acacggcgag
     2281 cgcgcggggg caggtggtcg cttgctgcat ctgggtgcgc aggtgctggt agccgcggcc
     2341 ctcccgactc cacgccgggc catcgatggc ggcgagcagg agatcgaggt cgaccggtgc
     2401 ctcgccgagg tcgaagcagt catcgagcca gagcagccag gcaagatccg tggcgagcag
     2461 cagggcgcag cgctcgtgga cggcgccgat cccggcgatc cagccgccgt ccccgtccgg
     2521 gatgccctcg atgtagctgt gcgcgaggac gaggccatcg cgggcgtggg tctcggtgaa
     2581 gccttcgagc tcgctccccc acgcgagcag atcgtgcagc tccgcgtcca gtgtggtcat
     2641 cggtcagcct ctgtgccgga agcggagcag ctggacgtcg atggcgcctt ccttccacgc
     2701 gcgcgagaac tggtgatgcc agtcgatcga cgtgcgcagc cgggcggcct ccaggtcgga
     2761 gagcttcccc gaggcgagca tccgcttgga ccaggcggcg ctctgcagcc agaacggggt
     2821 ggtgagctgc gttacatcct cggcgcggtc gagcacgaac ccggcggcgc gcgccgcgga
     2881 gacgtactcg tcgaccgggg cgatgtcggt atgccagtag ttgtcgaacg gggtcttcca
     2941 ctcgggcgag ccgaggaagg tatcctcgat gcagacaaag ccacccgggc ggatcagctc
     3001 ggcgaggtgg cggaaccact tccagcgcgg aaagtagcag gcgctctcca tcgccacggc
     3061 accgtcgtag cggcgcccgg tcgggacctc acagcagtcg gacagtacgg tctcgatgcg
     3121 gttcgcgact cccgcttgcg ccgcgaagct ctggatcagc tcgatgtgct ccgatgcgat
     3181 cgtgaccgag gtcacccgag ctccatgctc cgcggcccag tagagcgagc ctccacccag
     3241 gccgcaaccg acatcgagga tgtctcccga gagcgcccag tcggcttccc acacctccgc
     3301 cgcgtgcgcg accatgcgct cctgcgcgga ggtgatctcc gtccggagct gctccatatc
     3361 gaggcccggg cgaaacggat ccttctcgta cagcccgatg tggaagtgaa cccgaggtcc
     3421 gggaccgtac ttcttcaaga tcgaggcggt cttgtgattg tagtaggcct tgacaccggc
     3481 gcagtcctgg ggccgctcgg tgagctccgg cgcctgtggc gttgcaggat ctttggcctt
     3541 cagggacgcg ccaggcccgg aggcatgccg ctcgtgggtc tcggtggtcg gttggatgga
     3601 gttcacgggt acttccgttc ctgttgtatt ttttctagta ttacggaata aattagattt
     3661 atcagtatta taaattacta attagatatg agatgagtgc ggtctgtaga aagttttata
     3721 ttcatgaaaa cagcgaccta gaggatcctg ccctgcccgt tggttagata tatctcatga
     3781 tgagatatat ctaacctagg gcagtgctgc gacccaccgg tgcgtcgcgc gccgctgcgg
     3841 cgctgctcga tcccgggcgc atgaccgcgc cgctggatcg atccctgcgg ctcgctcggt
     3901 cacacacgtg cgcgtgaagg cgatccggcg ctgccgggag tctcacggag ccgacggtct
     3961 cgcgattcgt ccatgtgcga atcgtcgacg tcgatgggct cgacctgggc acgggcgtcg
     4021 gtgtgccgct cgcccgaccg cgtgatactg tgcctcgtcg ccggtgccca aggttctggt
     4081 ctcgaacaac tccgagctgc tccgccactt cacggcgcgg ccgttccagc ggctcgatct
     4141 gcagctgatc gtcgccggct cggcggcgga ggcccacgcg ctgttccgcc aggagcagcc
     4201 ggcgctcgcg gtgatcgacg ccgagcccgg cgggttcgac accgcgcggg cgatcaaggc
     4261 cgacaacccc gcgacccgcg tcgtgctggt cgccggcaag cagctgtcgg gcgaccagat
     4321 gcggcaggtc tcgatctcgg gctgcgacga gctgctgatc gcgccgatga ccgccgacga
     4381 gctccacgac gtggtcgcga tccagctcgg cgagccgcgg ccgggcagcg aggcgttctc
     4441 ggtcgccgtc cagctggcgg gcaagccggt cgacgccacg gtgtcgaacc tgtcggtcga
     4501 cggcgtgcgg ctggtggtcg gcgagccggt cagcgagggc caggcgctcg agctgtcgat
     4561 cgcccccgag ggcgacgcgc cggtcgagat ccgcggaacg gcggtgtggg cgcagccgcg
     4621 cgacggcaag accgtggtcg gcgtcgcgtt cgacaagccc gacgaccggg cgcgcgcggt
     4681 gctcgcccgg ctgacccagt ggcaggtggt caaggacggc gagcggatcc gggtcgtgct
     4741 gcgcggcgac ttcaccgagg cgacccggtt cgacgagctc ttgccggcga tggtcggccg
     4801 cgtcgtgttc gacaccgcgc aggtcacgta catgaactcg ctcggcgtgc gcgcgtggtg
     4861 cgagttcctg cgccaggcgc ggatccaggg ctacgagttc cacgcctgct cggtgccgtt
     4921 catcctccag gcgtcgatgg tgcgcgacgt gatcggccgc ggcaccgtga cgtcgttctt
     4981 cgcgccgttt cactgcatcg cctgcgacca ccaggaggag cgcctgctgc agtcggccgc
     5041 gatcctggcg tcgaacctgg agccgccgac gttcaagtgc ccgcgctgcg gcggcgcgct
     5101 ggagttcgac gacctgcccg agcgctactt cgcgttcctc caggacgatc cggagtgacc
     5161 ggcgtccagc ggtgcgctgc gcgtcagtgc ggcgtgcagg cgtacggatg cgccgtggtg
     5221 cacgcatcgg agtacagctt gccgctgt
//
LOCUS       DASVOR010001875         2597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_20799,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001875 DASVOR010000000
VERSION     DASVOR010001875.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20799"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1943)
                     /locus_tag="VF516_34805"
     CDS             complement(165..1943)
                     /locus_tag="VF516_34805"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEX8112971.1"
                     /translation="MTSTRRRWIAPAPTVVAWLLLAGLSGACSVRRTEPRADLILMNA
                     RVYTLTWGEPAADGTPAAGAPHGPAGWTPDAQAVAIRDSRIVFVGSNAAAAEWRGPSS
                     RVIDLHGATLVPGLVDSHVHILELGRKLERVNLVGVAGEAEAVERVAARAAVTPRGEW
                     ILGYGWDDGAWANHYPTLTLLSERVPDHPVYLRGLHGYAVWGNRLAFERAQITPATRA
                     PAGGEIVRDASGAPSGVLINAAVRLLESAMPPPTAAQVEALVLKGLEAMAQAGYVAVQ
                     EAGADSELMAAFEHLAATRRLPVRVYVMLSGRDHPLLERWLAKGRETPAEQRMLTTLG
                     VKVFFDGALGSRGARLLAPYADRPGERGVATPEAAFDPKLTAAMMKAGFQVDIHAIGD
                     AANREALDFVAGVMADAPSARSGRHRIEHAQVLDAHDVPRFAASGVIASMQPPHAVED
                     MTWAEDRLGADRVTLAYAWRTLRRSGAHLVFSSDLPGTDHDVFYGLHSAITRQDRAGK
                     PEGGWHAEQRMTPEEALRGYTTWAAYAAFAEDQTGVVAAGRWADLTAIDIDPLSAGET
                     DPARLLAGKIKMTMVAGRIVHQDSEL"
     gene            complement(2009..2179)
                     /locus_tag="VF516_34810"
     CDS             complement(2009..2179)
                     /locus_tag="VF516_34810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112972.1"
                     /translation="MKKTVKPLKVRKLMIDKETLRQLVVGAGAVPVPFTQVSCVVSQC
                     PNTCSNIFETCA"
     gene            2344..>2597
                     /locus_tag="VF516_34815"
     CDS             2344..>2597
                     /locus_tag="VF516_34815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112973.1"
                     /translation="MVRSERERERREDFFGSGGRLLVVGLVELRAADVRPARFTAVLG
                     TSATRAAAFVVGRAAARTAAVIVAVTPASRRAVGFGVAGFG"
BASE COUNT          329 a          963 c          932 g          373 t
ORIGIN      
        1 cgccgagcgc gagcggcgcg atcagaccga ggaggatctg gacggcgcgc ggtcgctcgc
       61 gaaacaaggg gggaggcttc acgccgccga atctacgccg cccgggccac cgccgggaat
      121 cgatctggct cgcggccgtt accggcgcgc tggacgcgaa gccctcagag ctcggaatcc
      181 tggtggacga tgcgtccggc gaccatcgtc atcttgatct tcccggccag cagccgcgcc
      241 ggatcggtct cgcccgcgct cagcgggtcg atgtcgatcg cggtgagatc ggcccaccgc
      301 ccggccgcca ccacgccggt ctggtcctcg gcgaacgccg cgtaggcggc ccacgtcgtg
      361 taaccgcgca gcgcctcctc gggcgtcatg cgctgctcgg cgtgccagcc gccctccggc
      421 ttgccggcgc ggtcctgccg cgtgatcgcg gagtgcaggc cgtagaacac gtcatgatcg
      481 gtgccgggga ggtcggagct gaacaccagg tgcgcgccgg atcgacggag cgtccgccac
      541 gcgtacgcca gcgtcacgcg gtctgcgccg agccgatcct cggcccacgt catgtcctcc
      601 accgcgtgcg ggggttgcat cgacgcgatg acgcccgacg ccgcgaaccg cggcacgtcg
      661 tgggcgtcga gcacctgggc gtgctcgatc cggtgccgac cgctccgcgc ggagggcgcg
      721 tccgccatga cgcccgccac gaagtcgagc gcctcgcgat tcgccgcgtc gccgatcgcg
      781 tggatgtcga cctggaaccc ggccttcatc atcgccgcgg tgagcttcgg atcgaaggcg
      841 gcctccggcg tcgcgacgcc gcgctccccg gggcggtcgg cgtacggcgc gagcagccgc
      901 gcgccgcgcg acccgagcgc gccatcgaag aacaccttca cgcccagcgt cgtcagcatc
      961 cgctgctcgg ccggggtctc gcgccccttc gcgagccagc gttcgagcag cgggtggtcg
     1021 cgtcccgaga gcatcacgta cacgcgcacc ggcaggcgcc gcgtcgccgc gaggtgctcg
     1081 aacgccgcca tcagctcgct gtccgcgccc gcctcctgca ccgcgacgta ccccgcctgg
     1141 gccatcgcct ccagcccctt gagcacgagg gcctcgacct gcgccgcggt cggcggtggc
     1201 atggcggatt cgagcagccg gaccgccgcg ttgatcagca ccccgctcgg cgccccggaa
     1261 gcgtccctca cgatctcgcc tcccgccggc gcgcgcgtcg cgggcgtgat ctgcgcgcgc
     1321 tcgaacgcga gtcgattgcc ccacacggcg tagccgtgca ggccgcgcag gtagaccggg
     1381 tggtccggca cccgttccga cagcagcgtc agcgtcggat agtggttggc ccacgccccg
     1441 tcgtcccagc cgtagccgag gatccactcg cccctgggcg tgaccgccgc gcgcgcggcc
     1501 acccgctcga cggcctccgc ctcgccggcg acgccgacga ggttcacgcg ttcgagcttg
     1561 cggccgagct cgaggatgtg gacgtgcgag tcgacgagac ccggcacgag cgtggcgccg
     1621 tggaggtcga tcacacggct ggacggtccg cgccactccg cggccgccgc gttcgacccg
     1681 acgaacacga tgcggctatc gcggatggcc accgcctgcg cgtccggcgt ccagccggcc
     1741 ggcccgtgcg gggcaccggc ggccggcgtg ccatcggcgg cgggttcgcc ccaggtcagc
     1801 gtgtagaccc tcgcgttcat gaggatcagg tcggcgcgcg gctccgtgcg gcgaaccgag
     1861 caggcgccgg acaggccggc gagcagcagc caggccacca cggtcggcgc cggcgcgatc
     1921 caccttcggc gggtgctggt catggggccg aatctacgcc tcccgggccg ccgccggatc
     1981 gccgcgagca gcttcgctca ggcagcgatc aagcgcaggt ctcgaagata ttcgagcagg
     2041 tattgggaca ctggctgacg acgcaactga cctgggtgaa tggcacgggg acagctccag
     2101 ctcccaccac cagctggcgc agcgtttcct tgtcgatcat cagcttccgg accttcagcg
     2161 gcttcacggt cttcttcatg ctcggctcct ggtctcgcgc gacgcgcggg gcgtccatgc
     2221 tactgcgtgc ccaggtgggc ggcgggcgac ctccgcgagt gcgtcgtcca tctcgcactg
     2281 cggtgtggga gacgctcggg ccttccccca ggtgggctcc ccgctcctca cactcagcgc
     2341 tgtgtggtgc gctccgagcg tgaacgcgag cgtcgcgagg acttcttcgg atccggcggg
     2401 cgactcttgg tggtgggcct ggtggagctg cgtgccgctg acgtgcggcc ggcgcgcttc
     2461 acggcggttc tcgggacgtc tgccacacgc gcggcggcct tcgtggtcgg ccgggcggcg
     2521 gctcgcacgg cggcggtgat cgtcgcggtg accccggcga gccggcgagc ggtgggcttc
     2581 ggcgtggcgg gcttcgg
//
LOCUS       DASVOR010001876         2003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_165524,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001876 DASVOR010000000
VERSION     DASVOR010001876.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165524"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2003
                     /locus_tag="VF516_34820"
     CDS             <1..>2003
                     /locus_tag="VF516_34820"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112974.1"
                     /translation="GLHPAAQRLLSALVVPDPMLRAPAAVLATGHPGQRTLWGRGISG
                     DEPIVWLRMDSRDPGELLGEVIAVHRHLRALGFRFDVIVADEAPSGYGDAPSQVLRRL
                     LAAHAADGLVHQRGGIHVVAVDQLSPQQLADLEAAAHVDLDARRGPLARQLEPGVPPA
                     ELPRFTPPGVPPGAAPDPELSLDVPELACDCGAGGFSGNDYVVRPGAVPPAPWCNVIA
                     NDRFGCLVSEGALGASWAGNAGENRLTPWHNDPVADPPAEVLYLRDEETARIWSTTPV
                     PAGGPTLVSHGHGHTTYRLACAGLDQTMTVFVPPDAPLKIIRLAIRNASARPRRVTAT
                     YYAEWVLGARRGETRLHIRPDLSAADACLLAETAWPMDFAGRVAFLASDRALHGYTTD
                     RLEMIGRGGDLSRPAGLVRWGLSGRVAPGADPCAALQVHLDLGPASGPDEATEVCFFL
                     GQADDRQHALALIRALRAPGAVAEAWRACQAYWDGVLDAVRVRTPDAALDQIANRWLL
                     YQTLAARWFGRTGYYQPGGAFGFRDQLQDCLAFLHVDPAWTRRHLLEAAAHQFEDGDV
                     LHWWHPPGGAGVRTRCSDDLLWLVYATAEYVAATGDTAVLGEPAPFLSGAALRPGEGA
                     RYDRFAAGSSGSLLEHCRRAMQRGLTAGPHGLPLIGDGDWNDG"
BASE COUNT          222 a          779 c          730 g          272 t
ORIGIN      
        1 cgggctccac cccgcggcgc agcgcttgct cagcgcgctc gtcgtcccgg atccgatgct
       61 gcgtgcgccg gccgccgtgc tcgccaccgg ccaccccggc cagcgcacgc tgtggggccg
      121 cggcatcagc ggcgacgagc cgatcgtctg gctccgcatg gacagccgcg atccggggga
      181 gctgctcggc gaggtgatcg ccgtccatcg tcacctgcgg gcgctcggct tccggttcga
      241 cgtgatcgtc gccgacgagg cgccgagcgg gtacggcgat gccccgtcgc aggtgctgcg
      301 ccggttgctc gccgcgcacg ccgcggatgg cctggtccac cagcgcggcg ggatccacgt
      361 cgtcgccgtc gatcagctct cgccccagca gctcgcggac ctcgaagcgg ccgcgcacgt
      421 cgatctcgat gcccggcgcg gcccgctcgc ccgccagctc gagcccggcg ttccgcccgc
      481 cgagcttccg cggttcacgc cgcccggtgt ccctcccggt gccgccccgg atcccgagct
      541 gtcgctcgac gtccccgagc tcgcctgcga ctgcggcgct ggcggcttct cgggcaacga
      601 ctacgtcgtg cgcccggggg ccgtgccgcc cgcgccgtgg tgcaacgtga tcgccaatga
      661 ccggttcggc tgcctggtca gcgagggcgc gctcggcgcc agctgggccg gcaacgccgg
      721 cgagaaccgg ctgaccccgt ggcacaacga cccggtcgcc gatccgcccg ccgaggtgct
      781 gtacctgcgc gacgaggaga ccgcgcggat ctggagcacg acgccggtgc cggccggggg
      841 cccgacgctg gtgagccatg gccacggcca caccacgtac cggctggcct gcgcggggct
      901 cgaccagacc atgacggtgt tcgtcccacc ggacgcgccg ctcaagatca tccggctcgc
      961 gatccgcaac gcgagcgcgc ggccgcgccg ggtgaccgcg acctactacg ccgagtgggt
     1021 gctcggcgcc cgccgcggcg agacccggct ccacatccgg cccgatctgt cagccgcgga
     1081 tgcctgcctg ctggccgaga ccgcgtggcc gatggacttc gccggccgcg tcgcgttcct
     1141 ggcgagcgat cgtgcgctgc acggctacac caccgaccgg ctcgagatga tcggccgcgg
     1201 cggcgacctg tcgcgtccgg cgggcctcgt gcgctggggg ctgtcggggc gggtcgcgcc
     1261 cggcgccgat ccgtgcgccg cgctgcaggt ccacctcgat ctcggtccgg cttccggccc
     1321 ggacgaggcc accgaggtct gcttcttcct cggccaggcc gacgatcgcc agcacgcgct
     1381 cgccctgatc cgcgcgctgc gcgccccggg cgccgtcgcg gaagcgtggc gagcgtgcca
     1441 ggcgtactgg gacggcgtgc tcgacgcggt ccgggtccgc acgccggacg cggcgctcga
     1501 ccagatcgcc aaccgatggc tgctctacca gaccctcgcg gcgcggtggt tcgggcggac
     1561 cgggtactac cagcccggcg gcgcgttcgg gttccgcgat cagctgcagg actgcctcgc
     1621 gttcctgcac gtcgatccgg cgtggacccg gcgccacctg ctcgaggccg cggcccatca
     1681 gttcgaggac ggcgacgtcc tccactggtg gcatccgccg ggcggcgccg gggtccgcac
     1741 gcggtgctcg gacgacctct tgtggctggt ctacgcgacc gccgagtacg tcgccgcgac
     1801 cggcgacacc gcggtgctgg gagagccggc gccgttcctg tccggcgcgg cgctgcgccc
     1861 gggcgagggc gcccgctacg atcgcttcgc cgccggttcg agcggatcgc tgctcgagca
     1921 ctgccggcgc gcgatgcagc gcggcctgac ggccgggccc cacggcttgc cgctgatcgg
     1981 cgacggcgac tggaacgacg gga
//
LOCUS       DASVOR010001877         1923 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_370437,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001877 DASVOR010000000
VERSION     DASVOR010001877.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1923
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370437"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1857)
                     /locus_tag="VF516_34825"
     CDS             complement(<1..1857)
                     /locus_tag="VF516_34825"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8112975.1"
                     /translation="MLFVDLTEQRHWVEDVDESVYRAYLGGYGLGAWLMWKHFPSGAD
                     PLSPQACFALSSGLLTGLKTPFSGRIQIVGKSPLTGTWADSNSGGSVCSHLRLAGYDA
                     LVVRGRAAAPTLLVIRDGEVSFEPAGELWGQEIPETFNALKARFGGKRDVGVSAIGPA
                     GEAQLRIASVMNDRYHAFGRQGFGAIYGSKNLKAIVVSGTGEIKVAKPAELKAICKQI
                     TDEYKRDLGLIARIFAYFAKPKRWLGWMYRMMTRMGSKMQSPTAAMRQLWSQRGTTAA
                     VALSIENGDAPIKNWRGVGTRDFPLATKGMKTDGAVVDKYITKKLSCGDCPMPCKGIV
                     KVKSKNLTDVRRPDYETLVGFGANLLNDDIELVTACHDACNRLGIDAVSSSATLAWAC
                     EAVERGDLTAADLDGIDLKWGNGEAALALTIKMGKGEGCGAWLGHGVARAAEHVGKGS
                     AEYAVHIHGGEPAYHDSRFSSLMGVTYVSDPTPGRHTAGSASWNETFGAGFSLPKAAD
                     RKDWNVKWKGTTGKGHAQALFSNAHQAMNGLGLCMFTMLTGSLPWADLLDAATGWGLT
                     EADLLVCGERIQNLRAAFNRREGLAPKDFQPHRRMLGEGDGQLEAGPLKGIRV"
BASE COUNT          267 a          685 c          665 g          306 t
ORIGIN      
        1 gacccggatg cccttgagcg ggccggcctc gagctggcca tcgccctcgc cgagcatgcg
       61 gcggtggggc tggaagtcct tgggcgcgag gccctcgcgg cggttgaacg cggcgcgcag
      121 gttctggatc cgctcgccgc ataccaggag gtcggcctcg gtcagccccc acccggtggc
      181 ggcgtcgagc aggtcggccc acggcagcga gccggtcagc atggtgaaca tgcacagccc
      241 caggccgttc atcgcctggt gggcattgga gaacagcgcc tgggcgtgac ccttgccggt
      301 ggtgcccttc cacttgacgt tccagtcctt ccggtcggcg gccttcggca ggctgaaccc
      361 ggcgccgaac gtctcgttcc acgacgccga gcccgcggtg tggcggccgg gcgtcggatc
      421 ggacacgtag gtcacgccca tcagcgacga gaaccggctg tcgtgatacg ccggctcgcc
      481 gccgtggata tggaccgcgt actcggccga gcccttgccg acgtgctcgg cggcgcgcgc
      541 cacgccgtgg ccgagccacg cgccgcagcc ctcgcccttg cccatcttga tcgtgagcgc
      601 gagcgcggcc tcgccgttgc cccacttgag gtcgatgccg tcgaggtcgg cggccgtcag
      661 gtcgccgcgc tcgaccgcct cgcacgccca cgcgagcgtc gccgacgacg acaccgcgtc
      721 gatgccgagc cggttgcagg cgtcgtggca cgcggtgacc agctcgatgt cgtcgttgag
      781 cagattggcg ccgaacccga ccagggtctc gtagtccggc cgccggacgt cggtcaggtt
      841 cttcgacttg accttgacga tgcccttgca cggcatcggg cagtcgccgc acgacagctt
      901 cttggtgatg tacttgtcga ccaccgcgcc atcggtcttc atgcccttgg tcgcgagcgg
      961 gaagtcgcgg gtgccgacgc cgcgccagtt cttgatcggc gcgtcgccgt tctcgatcga
     1021 cagcgcgacc gccgcggtcg tgccgcgctg cgaccacagc tggcgcatcg ccgcggtcgg
     1081 cgactgcatc ttgctgccca tccgcgtcat catccggtac atccagccca gccagcgctt
     1141 gggcttggcg aagtacgcga agatccgcgc aatcagtccg aggtcgcgct tgtactcgtc
     1201 ggtgatctgc ttgcagatcg ccttgagctc ggcgggcttg gcgaccttga tctcgccggt
     1261 gcccgacacg acgatcgcct tgaggttctt gctgccgtag atcgcgccga agccctggcg
     1321 gccgaacgcg tggtagcggt cgttcatcac cgaggcgatc cggagctggg cctcgcccgc
     1381 cgggccgatc gccgacacgc cgacgtcgcg cttgccgccg aaccgggcct tgagcgcgtt
     1441 gaacgtctcc gggatctcct ggccccacag ctcgcccgcc ggctcgaagc tgacctcgcc
     1501 gtcgcggatc accagcagcg tgggcgccgc ggccctgccg cgcacgacca gcgcgtcgta
     1561 gccggcgagc cgcaggtggc tgcacacgct gccgccggag ttggagtcgg cccaggtccc
     1621 ggtcagcggc gacttgccga cgatctggat ccgccccgag aacggcgtct tgagcccggt
     1681 caagaggccc gagctcagcg cgaagcaggc ctggggcgac agcgggtcgg cgccggacgg
     1741 gaagtgcttc cacatgagcc acgcgcccag gccgtagccg ccgaggtacg cgcggtacac
     1801 cgactcgtcg acgtcctcga cccagtggcg ctgttcggtc agatcgacga acagcacctt
     1861 gccgaggtgg ccatagcggg gacgaacgag cgctgcatcg gaagctgcgg gcgatgccat
     1921 ggg
//
LOCUS       DASVOR010001878         3126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_49313,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001878 DASVOR010000000
VERSION     DASVOR010001878.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3126
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49313"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(867..2357)
                     /locus_tag="VF516_34830"
     CDS             complement(867..2357)
                     /locus_tag="VF516_34830"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEX8112976.1"
                     /translation="MNPSVFREYDIRGVADRDLDDATVRALGTAMGAQAAAAGGPVVV
                     GRDPRVHSPRLFGALTDGLRVHAEVIDIGVVPTPVLYFAAHHLASAAAVMITGSHNPP
                     EDNGFKLVAGGETLHGAAIAALRDRVQQLIAEPAPHPIKAMHSRDVIGPYIDHAQAML
                     RLGPRRPRVVIDAGNGAGGPTALALYRRLGFDVVPLYCELDGTFPHHHPDPTVPAHLA
                     DLIAEVHRTGAELGIAFDGDADRLGVVDATGRILWGDQLMILLGKAVLREVPASRFVG
                     EVKCSQAMYDELAAAGGKVEMWKVGHSLIKARMKQIGAALAGEMSGHLFFAHRWLGFD
                     DAIYAGARVLELLSRGHRTLRELADELPVMINTPEIRVDCPDGDKFAVVAEVAARLRA
                     DPAVTGVVDIDGVRARFTGGWGVVRASNTQPALVMRCEATTAERLAEIRAAIEAHVAA
                     ARAARGEDVQRVARGERAHASSPGAAARMGVGAASAESPPVLRGPL"
     gene            complement(2361..>3126)
                     /locus_tag="VF516_34835"
     CDS             complement(2361..>3126)
                     /locus_tag="VF516_34835"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX8112977.1"
                     /translation="AVLAGRDRSAVLAVLPADQHVADEAGLTAVLDTALAAVERDDVI
                     GTVGIPPTRAETGFGYLEVERAAPGLVTPVLRFVEKPDRPTAEAYVASGRYLWNAGMF
                     CVSADRLLRELDDQLPATGRAVRDLAAGRPGASEAYAALVSISIDHAVMERAARVVTV
                     PAAVGWDDVGSWAALRALRGTDGDGNTAAGTAVIIGGTGNVAIGDGDTLIAMVGVSDL
                     VVVQSGDAILVLPRSEAQDVRKIVEALSARGLARYL"
BASE COUNT          431 a         1131 c         1147 g          417 t
ORIGIN      
        1 atcgacggca ggggcgcttc ggtcgcgctc cgcagctccg accctcacac ggccagcacg
       61 tcgcgcgccc ctgcttgcct tacacctcac accacccctg ggcgtccgcc attccggact
      121 ggtaggtgat tgacaaccgc agctacgaac accgatggca ctgcgctggg tggtgccggt
      181 ggcaggccca ccgggaatcc gatgctcacg cccctgcgcg gcggttgcga gagcggcacg
      241 tttctcggca cctcggccac cgctggcgga ggcggtgcaa ttcagctggt gagtcgcacg
      301 aagattctcg tcaatggcgt tgtagcagca aatggcagct cgttcaccgg cggcggatcg
      361 ggtgggggta tcttgctcga agcaccgctc gtggacgttt caggtagcat ggtggccaac
      421 gggggtgcgg gcgcgggtgg ctgcctgctg ccccaggtag gagaagatgg tcacctggac
      481 gcagcgccgg cgataggcgg aggcacagga tgcggcggat ccggcgcgaa cggcggcaac
      541 ggtggagcag ggtccctggc cgctggtggg gggatgagta ttagcgtagt cgctggcatt
      601 tcagaccccg catttggcgg cagcggcggt ggcggagtcg ggcggatccg gatcaacacc
      661 gctccgggcg gcttccaccg ggccgggctg ttctccccca accccagcac cggcgcgatc
      721 gccacccgct gacggcgtcg ccagccagct cgtcgagaca cgatcgcggg acgccaacgc
      781 gagctcgccg ggggcgaccg cgagcaggat ggggtcggac gccgcggccg tgatttggat
      841 tggccgttcg ttgcccccgc acctacctag aggggtccgc gcaggacggg tggtgattcg
      901 gcggacgcgg ctccgacacc catccgcgca gcagcccccg gcgagctcgc gtgtgcgcgt
      961 tctcctcgcg ccacgcgctg cacgtcctcg ccgcgcgcgg cgcgggcggc ggcgacgtgg
     1021 gcctcgatcg ccgcgcggat ctcggcgagc cgctcggcgg tggtggcctc gcagcgcatg
     1081 accaaagctg gctgggtgtt ggatgcgcgg accacgcccc agccgccggt gaaccgggcg
     1141 cgcacgccgt cgatgtcgac cacgccggtc accgcgggat cggcgcgcag ccgcgcggcg
     1201 acctcggcga ccaccgcgaa cttgtcgccg tcggggcagt cgacccggat ctcgggcgtg
     1261 ttgatcatga ccggcagctc gtcggcgagc tcgcgcagcg tgcggtggcc gcgcgacaag
     1321 agctcgagca cccgggcgcc ggcgtagatc gcgtcgtcga acccgagcca gcggtgggcg
     1381 aagaacaggt ggccggacat ctcgccggcg agcgccgcgc cgatctgctt catccgcgcc
     1441 ttgatcagcg agtggccgac cttccacatc tcgaccttgc cgccggcggc ggcgagctcg
     1501 tcgtacatcg cctgcgagca cttgacctcg ccgacgaacc gcgacgcggg gacctcgcgc
     1561 agcaccgcct tgccgagcag gatcatcagc tgatcgcccc acaggatccg gccggtcgcg
     1621 tcgaccacgc cgagccggtc ggcgtcgccg tcgaacgcga tgccgagctc ggccccggtg
     1681 cggtggacct cggcgatcag gtcggcgagg tgggcgggca cggtcggatc cgggtggtgg
     1741 tgcgggaacg tgccgtcgag ctcgcagtac agcggcacga cgtcgaaccc gagccggcgg
     1801 tacagcgcca gcgcggtcgg cccgcccgcg ccgttgccgg cgtcgatcac gacccgcggt
     1861 cgccgcgggc cgagccgcag catggcctgc gcgtggtcga tgtaggggcc gatcacgtcg
     1921 cgcgagtgca tcgccttgat cgggtgcggc gccggctcgg cgatcagctg ctggacccgg
     1981 tcgcgcagcg cggcgatcgc ggcgccgtgc agggtctcgc cgccggcgac gagcttgaag
     2041 ccgttgtcct cgggcgggtt gtggctgccg gtgatcatca ccgccgcggc ggacgcgagg
     2101 tggtgcgccg cgaagtacag caccggggtc gggaccacgc cgatgtcgat cacctcggcg
     2161 tggacgcgta gcccgtcggt caacgcgccg aacagccgcg gcgagtggac ccgcggatcg
     2221 cggccgacga cgaccggccc accggccgcg gcggcctgcg cccccatcgc ggtccccagc
     2281 gcgcgcaccg tggcgtcatc gagatcgcga tccgcgacgc cgcggatgtc gtactctcga
     2341 aacacggaag ggttcaccgg tcataggtac cgcgcaagcc cgcgcgccga aagagcctcg
     2401 acgatcttgc gcacgtcctg ggcctcggac cgcggcagga cgaggatcgc gtcgccgctc
     2461 tggaccacga ccaggtcgga cacgccgacc atcgcgatca gcgtgtcgcc gtcgccgatc
     2521 gcgacgttgc cggtgccgcc gatgatcacc gccgtgccgg ccgcggtgtt gccgtcgccg
     2581 tccgtgccgc gcagcgcgcg cagcgccgcc cacgacccga cgtcgtccca gccgaccgcg
     2641 gccgggacag tcaccacgcg cgcggcgcgc tccatcaccg cgtggtcgat cgagatcgac
     2701 accagcgccg cgtaggcctc gctcgccccg ggccggccgg ccgcgaggtc gcgcaccgcg
     2761 cgcccggtcg ccggcagctg gtcgtcgagc tcgcgcagca ggcggtccgc cgacacgcag
     2821 aacatccccg cgttccacag gtagcggccg ctcgcgacgt aggcctccgc ggtcggccgg
     2881 tccggcttct cgacgaaccg gagcaccgga gtgaccagcc ccggcgccgc tcgctcgacc
     2941 tcgaggtagc cgaacccggt ctcggcgcgg gtcgggggga tgccgacggt gccgatcacg
     3001 tcgtcgcgct cgaccgcggc cagcgcggtg tcgagcaccg cggtcaggcc ggcctcgtcg
     3061 gcgacgtgct gatcggcggg cagcaccgcc agcaccgcgc tgcgatcgcg cccggcgagc
     3121 acggcg
//
LOCUS       DASVOR010001879         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_49317,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001879 DASVOR010000000
VERSION     DASVOR010001879.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49317"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /gene="ltrA"
                     /locus_tag="VF516_34840"
     CDS             complement(<1..1417)
                     /gene="ltrA"
                     /locus_tag="VF516_34840"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:TIGR04416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX8112978.1"
                     /translation="MKSLGQVMYVATKEDTLWLQNVQHTLYTRSKEHLDYVFEKLWGL
                     VTDPRNLRIAFARVQRNRGARTAGIDRVTVRRVQRSGVTPFLDEIRKDLRGGEFRPDA
                     VRRVLIPKAGQPGTFRPLGIPTVKDRVVQAAVKNIMEPIFEADFYPVSYGFRPGKSVH
                     GAIAHLKELMRSRGGKQWKRAEKLPFQWAIEGDIKGCFDNIGHHGLMERVRRRVGDAK
                     LERLVLAFLKAGVLSEAQFLRSDSGTPQGGILSPLLANIALSIIEERYERHAWPRGTG
                     TRLSPAGTPSVPRTDPKAAARRAAENRSRDKRRGRPVFMPIRYADDFIVLVATGGDPQ
                     ETRAVAEAEKAALADELRRKLGLVLSEEKTLVTPVTSTMRFLGHHINVRAQPDDGRFA
                     PRVVIPKERSKQLRHKIKNLFRRSTCGRTLESLLRLANPILRGWANFYRHAWGAKQVF
                     CANDHYVWWTILRWLRKKHPQT"
     gene            complement(2211..>2429)
                     /locus_tag="VF516_34845"
     CDS             complement(2211..>2429)
                     /locus_tag="VF516_34845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112979.1"
                     /translation="LDAAVLAGFLLEHEDDVETALARYEADMFPRAAEAARQSAFGLD
                     LIFHADAPRGLVDFFTSMPEPVEAGADR"
BASE COUNT          399 a          821 c          723 g          486 t
ORIGIN      
        1 gagtctgggg atgcttcttg cggagccatc gcaggatggt ccaccacacg tagtggtcgt
       61 tggcgcagaa gacttgcttc gcgccccacg cgtgtcgata gaagttcgcc caaccccgca
      121 ggatggggtt cgccaagcgc agcaggctct caagagttct gccgcaggtt gacctgcgaa
      181 agagattctt gatcttatgg cgcagctgct tcgatcgctc tttcgggatc accaccctcg
      241 gcgcgaaacg accgtcatcc ggctgtgcgc gcacgttgat gtggtgcccg agaaagcgca
      301 tcgtgctcgt caccggcgtg acgagcgtct tctcctcgga aagaacgagc ccaagcttgc
      361 gcctgagttc gtccgccaag gctgccttct cggcttcggc gaccgcacgg gtctcctgag
      421 gatccccgcc ggttgccacg agcacgatga agtcatcggc gtaccggatg ggcatgaaga
      481 ctggacgacc gcgtcgcttg tcacgggagc ggttctcagc cgcccgacga gcagccgcct
      541 tcggatcggt ccgtggcacg cttggggtcc cggcggggga caggcgagta cctgtcccgc
      601 gcggccaagc gtgccgctcg tacctctcct cgatgatgct cagcgcgata ttcgccagca
      661 ggggagaaag aatcccaccc tgtggtgttc cgctatcgga ccgcaagaac tgcgcctcgg
      721 acagaacgcc agccttcagg aatgccaaga cgagtcgctc gagcttcgcg tcgccgacgc
      781 gtcgccgaac gcgctccatc agcccgtgat ggccgatgtt gtcgaagcat cccttgatgt
      841 cgccttcaat tgcccactgg aaggggagtt tctccgcccg cttccactgc tttccgcccc
      901 gtgagcgcat cagctccttg agatgagcga tggctccgtg aacggactta cccggccgga
      961 agccgtacga gacggggtaa aaatccgcct cgaagatcgg ctccatgatg ttcttcaccg
     1021 cggcctgcac taccctgtct ttcacggtcg ggattcccaa gggtcgaaac gttcccggtt
     1081 gtcctgcttt cggaataagt acgcgccgga cggcatccgg ccggaactcg ccgcctcgca
     1141 ggtccttccg gatctcatcg aggaacggcg ttaccccgct ccgctgaact ctcctgaccg
     1201 tgacccggtc tatgcccgcc gtccgcgcgc cgcggtttcg ctgcacgcgg gcgaaggcga
     1261 ttcgaagatt ccgaggatct gtcaccagac cccacagctt ctcgaacacg tagtccaggt
     1321 gctccttact tcgcgtgtac agcgtgtgtt gcacgttctg gagccaaaga gtgtcctcct
     1381 tggttgccac atacatgact tgccccagcg acttcaccgc tgtactcatc tactgcctgc
     1441 cttcgccatg tgcacggctc tcccgtgctc ggactactac gaaggctccg cccctcgacc
     1501 gagacgtcgc cgggcttgtc ggcttgccgg atgtttccct cccggcgctc ggctcgaggt
     1561 tcttacgttc ccgtcggaga cccttggtgc ggtaggtgat cagctttgcc cctggcagct
     1621 ctggccgccc gtcgcttcgg gaccagatag gcggcgcatc ggctaggccg acgctccaga
     1681 tcgaacggcg tgtccatccg gatcgcactg cattacccac ctccaggacg agggggacat
     1741 ctctacctac agagggatca gacgctgatt tcaactgtgt acccatgtta cacgtggttc
     1801 accgtggcac ctgtggtagc tcatcggtcg aggatttctc gtcgccatga gcagttcacg
     1861 ccgctcggct tttgcagtcc gccatttcca accttctggc ggggttcgcg gcgcccttac
     1921 gcgctcgacc cgactcgagc ggtgatgacg taacatactg tcaggtcatc ggtctcctcc
     1981 agtcttcgta acgcaccgtc cgctgagatc tgccatctgc tcctcggagc cgaagcctag
     2041 acgccgcccg aaccatacga acaccgagcg aacgctgatt ttcctgccgc atcttgctgc
     2101 gcccgtaatc cgctcccgct gcttcgtgtg cgtgctccca ccttgcccgc gtcggaacca
     2161 cctcctggcg accgatgggg ccggccgcta tggctatggc cttcgggctc ctatcggtcg
     2221 gcgccggcct ccacgggctc gggcatcgag gtgaagaagt ccaccaggcc ccggggcgcg
     2281 tcggcatgga agatcaggtc gagcccgaag gcggactgcc gggccgcctc ggcggcgcgc
     2341 gggaacatgt cggcctcgta gcgggccagc gcggtctcga cgtcatcctc gtgctccagc
     2401 aggaacccgg ccaggacggc cgcgtcgag
//
LOCUS       DASVOR010001880         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_312252,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001880 DASVOR010000000
VERSION     DASVOR010001880.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312252"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2043
                     /locus_tag="VF516_34850"
     CDS             <1..2043
                     /locus_tag="VF516_34850"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8112980.1"
                     /translation="KARQIATQPLSGVAGESRCWATERCIDPHPYSPCEWLPDSRALV
                     CRTVAPDVKPPPAAPSAPSGPIVQENLGSKKPAPTNPELLSSPADERMFEYYMTSQIA
                     LVTVDGKVSPIGAPGLYLRSAPSPDGKWLLVESVHRPFSYRVTLRQFPARTEVWRLDG
                     KLRAMIADLPLAEEVPSVRDAVRTGRRDIQWRADAPATACWVEAQDGGDPRTKVAVRD
                     LVQCAAEPFTRPVRLAELADRFRGIEWGNGKLALVSDGRWKDRHTRTSIVAPEDPAAK
                     PRVLWDRSSEDRYTDPGRPVLRPAPSGHPVLQVTSKGELLLAGEGASAQGDQPFLDRL
                     DLATGKTERIWRSDGERFARVVAVVDPDGGDAIVSREAPTTPPQLYRTSFAGKTERQL
                     THVTHPVPELAQVKKQLIKWKRKDGLELSGMLYTPAGFRPGKDRPLPVLVWVYPQEFK
                     SASAASEVRGSPYQFVFPQWGGPLFALTQGYAVVDNPTLAIIGEGKTEPNDTYVEQLA
                     DGAASMIDAVVALGVGDRDRFAVGGHSYGAFTTANLLAHTDLFRAGIARSGAYNRTLT
                     PFGFQSEERTFWEAAATYDRMSPFRYADKINEPLLMIHGAADNNDGTFPIQSDRLFAA
                     LQGLGGHVRYVSLPAEAHGYRARESALHVLWEQVRWLDTHVKNARPRPAPRVTAAP"
     gene            2116..>2416
                     /locus_tag="VF516_34855"
     CDS             2116..>2416
                     /locus_tag="VF516_34855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8112981.1"
                     /translation="MQPTKILVADDEQNLRRVLVAMLRRDGHDVVQAASGIEAIERLP
                     DVDVVITDLRMPGADGMEVLRTAAWSYPQVPVIMITAYGSVGQAVEAIKAGAFDYI"
BASE COUNT          349 a          887 c          856 g          324 t
ORIGIN      
        1 aaggcccggc agatcgcgac gcagccgctg tccggcgttg caggcgaatc tcggtgctgg
       61 gcgaccgaac gctgtatcga cccgcacccg tactcgccgt gcgaatggct ccctgactcg
      121 cgcgcactgg tgtgccgcac cgtcgcgccc gacgtcaagc cgccgccggc cgcgccgagc
      181 gcgccgagcg ggccgatcgt ccaggagaac ctcggcagca agaagccggc gccgaccaac
      241 cccgagctgc tgtcgagccc ggccgacgag cggatgttcg agtactacat gacctcgcag
      301 atcgcgctgg tcaccgtcga cggcaaggtc tcgccgatcg gcgcgcccgg cctgtacctg
      361 cgctcggcgc cgtcgcccga cggcaagtgg ctgctggtcg agtccgtgca ccggccgttc
      421 tcgtaccgcg tcacgctccg ccagttcccc gcccggaccg aggtgtggcg gctcgacggc
      481 aagctccgcg ccatgatcgc cgatctgccg ctcgccgagg aggtgccgag cgtgcgcgac
      541 gcggtgcgga ccgggcggcg cgacatccag tggcgcgcgg acgcgccggc caccgcgtgc
      601 tgggtcgagg cgcaggacgg cggcgatccg aggaccaagg tcgcggtccg cgatctcgtc
      661 cagtgtgcgg ccgagccatt cacgcgcccg gtgcgcctgg ccgagctcgc ggaccggttc
      721 cgcggcatcg agtggggcaa cggcaagctc gcgctggtca gcgacggccg gtggaaggat
      781 cgccacaccc gcacgtcgat cgtcgccccc gaggatccgg ccgcgaagcc gcgcgtgctg
      841 tgggatcgct cgtcggagga tcgctacacc gaccctggcc ggccggtgtt gcgtccggcg
      901 ccgagcggac accccgtgct ccaggtgacg agcaagggcg agctgctgct cgccggcgag
      961 ggcgcctcgg cccagggcga ccagccgttc ctcgaccggc tcgacctcgc gaccggcaag
     1021 accgagcgga tctggcggtc cgacggcgag cggttcgccc gcgtggtggc cgtggtcgac
     1081 ccggacggcg gcgacgcgat cgtgtcgcgc gaggccccga ccacgccgcc ccagctctac
     1141 cggacgtcgt tcgccggcaa gaccgagcgc cagctgaccc acgtcaccca cccggtgccc
     1201 gagctcgccc aggtcaagaa gcagctgatc aagtggaagc gcaaggacgg cctggagctg
     1261 tccggcatgc tgtacacgcc ggccgggttc cgccccggga aggaccggcc gctgccggtg
     1321 ctggtctggg tctacccgca ggaattcaag tccgcgagcg ccgcctccga ggtccgcggc
     1381 tcgccgtacc agttcgtgtt tccgcaatgg ggcggcccgc tgttcgcgct gacccagggc
     1441 tacgccgtgg tcgacaaccc gacgctggcg atcatcggcg agggcaagac cgagcccaac
     1501 gacacctacg tcgagcagct cgccgacggc gcggcctcga tgatcgacgc ggtcgtcgcg
     1561 ctcggggtcg gcgatcgcga tcgcttcgcg gtcggcggcc actcgtacgg cgcgttcacc
     1621 accgcgaacc tgctggccca caccgacctg ttccgcgccg gcatcgcccg gtcgggcgcg
     1681 tacaaccgca cgctgacccc gttcgggttc cagtccgagg agcgcacgtt ctgggaggcc
     1741 gccgcgacct acgaccggat gtcgccgttc cgctacgccg acaagatcaa cgagccgctg
     1801 ctgatgatcc acggcgcggc cgacaacaac gacggaacgt tcccgatcca gagcgaccgg
     1861 ctgttcgccg cgctgcaggg gctcggcggt cacgtccgct acgtgtcgct gcccgccgag
     1921 gcgcacggct accgggcccg cgagtccgcg ctccatgtcc tgtgggagca ggtccgctgg
     1981 ctcgacaccc acgtcaagaa cgccaggccg cgaccggcgc cccgggtcac cgccgcgccg
     2041 tgaccggacc ctgcgtgtga ggccggggcg cggccgtggc gcacgcgggc gcgggatgcg
     2101 gtagggtaat ttgccatgca accgacgaag atcctggtcg ccgatgacga gcagaacctt
     2161 cgtcgcgtcc tggtcgcgat gctgcggcgc gacggccacg acgtcgtgca ggccgccagc
     2221 gggatcgagg cgatcgagcg cctgcccgat gtcgacgtcg tgatcaccga cctgcggatg
     2281 ccgggggccg acggcatgga ggtgctgcgc acggccgcct ggtcgtaccc ccaggtcccg
     2341 gtcatcatga tcaccgcgta cggctcggtc ggccaggcgg tcgaggcgat caaggccggc
     2401 gcgttcgact acatcg
//
LOCUS       DASVOR010001881         9110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340693,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001881 DASVOR010000000
VERSION     DASVOR010001881.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9110
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340693"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="VF516_34860"
     CDS             complement(<1..1295)
                     /locus_tag="VF516_34860"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX8112982.1"
                     /translation="MRIRFDRGTLVLEAEHEGEQPGAIAGVAWDEERRAWRLPAEHHA
                     ALRRELADAGVPVSDELGGHAVAMTWMMPGLRWYQEVALERWLAAGGRGVVALPTGAG
                     KTLVAIAAIARLGVATLCLVPTRVLLDQWARALAVCTAQPIGRLGDGDHVVAPVTVAT
                     YASATSWAPRIGGRFGLVIVDEAHHVGAWCPTEVFEMLVAPARLGLTATPPEDRAVLA
                     RHVGPVVYALTVADLRGDGLADHQLVTVPVALDGAERRRYRELRGRFAKHYAAFQRAM
                     PDAAWREFVREALQTVQGRAALAAWREYRALLAYPDGKRAALRQILGQHRGSRALVFT
                     ADNATAYAIARELLVMPITHEIRRTERTRALAGFRTGECPVLVSSQVLEEGFDVPDAD
                     LAIVVGGSGSPRRYVQRIGRVLRPRDGKHALIYELAVAET"
     gene            complement(1669..2412)
                     /locus_tag="VF516_34865"
     CDS             complement(1669..2412)
                     /locus_tag="VF516_34865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112983.1"
                     /translation="MAGPTALLAMARGPILTAISTAASATRWVSATPFADRSNDNYGC
                     QESIEIGSSASGSADAMRGLVQFDLSSLTAFPFVQKAFLEMTLFGFDRGLPDVASVYT
                     IGAFRIIPSGTRTPWLEGNGVEGDVVPLGCTNVNDASGVAWFGNGDGGDPDNQTQPDF
                     DPQVLATATIVQASNVPGDVFRWDVTDLVRAWTSRTTPNFGIMLRETSGDGSFRGVRF
                     GARDGFRDFSPGAVAGPRLIVTLTPQRLP"
     gene            complement(2952..3422)
                     /locus_tag="VF516_34870"
     CDS             complement(2952..3422)
                     /locus_tag="VF516_34870"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX8112984.1"
                     /translation="MTRTHVFDAHLETANKWMDEVGESLELPATERPRALHALRAGLH
                     AIRDRLPAAEVVDLGAQLPTLIRGFYYEGWTLANDPTRMRDRADMIARVKKELMLDPR
                     LDPVDVLRAVIHLLVEHVSPGEIRHIVSTLPRPIASLWQDLTGHALDMLGANQS"
     gene            complement(3580..6030)
                     /locus_tag="VF516_34875"
     CDS             complement(3580..6030)
                     /locus_tag="VF516_34875"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8112985.1"
                     /translation="MPTELRPDELFRSCDPRDIPFDSTADEPEPIQVLGQARAIEAVR
                     VAVGIRHDGYNLFALGPAGVGKHTTLRQMLEREAARNAAPVDWCYVNNFVEPRRPRAL
                     RLPAGAATRLRANMEHAVAELRVAIPAAFDSGEYRNRKQQLGQDFQRRQAAAFEELQS
                     RARARRVGVMHTDGGFALAALRRTEAGDEAIDAEAFGRLPDHEQAELRAAMEQVGAEL
                     TALLRTFHEWAREQLEALHALDRATAAAVARRVVDAVRAGYHELPAVLAYLGEVENDV
                     IDGAGRFLPGSDDEVPEALRRVLHHHGDEARFRRYAVNVVVDHAAARGAPVIYEDNPT
                     HPNLIGRIEHVAELGALITDFTLIRPGALHRGNGGYVLLDALKLLQQPLAWEALKRAL
                     RTHEIRIESLGEALGLASTVSLEPEPIPLEIKVVLLGDRLLYYLLAAHDPEFGDLFKV
                     MADFEQDIDRSRDADVAYARLIAGLVRQHGLRPFDRGAVARAIEHASRLASDGAKLSL
                     HVRSISDLLHEAERCATDAGRSTATAGDVQAAIDAQLHRAGRIRERMLEAVRRGTVLI
                     ETSGEVVGQINGLSVVELGDQAFGHPTRISARARIGDGKVVDIEREVELGGPIHSKGV
                     LILAGLLGARYAEHVPLALSATIVFEQSYGGVEGDSASLAELCALLSAIADAPVKQSL
                     AVTGSVNQHGALQAIGGVNEKIEGFFDVCSQRGLTGEHGVVIPRSNVQHLMLRKDVVA
                     AVAEGKFHVHAVATADEALELVTGRIAGARDHDGHFPVGSVNALVEARLLRFADDIRR
                     FAAGTERPAAASRVRHWS"
     gene            6193..6888
                     /locus_tag="VF516_34880"
     CDS             6193..6888
                     /locus_tag="VF516_34880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112986.1"
                     /translation="MRVSAFLILALAACEPSHASPAVPGDHEATVRSHMREHYDLFSA
                     IQHVVIRNDLDTTRALARSIGHAPAGPGLDRWATQAALARARAAELAAAPGTDEACRR
                     TARLAEACARCHIDAGVVPMFGSPPPLPGEAADKDRMALHLWAAERLSEGMIGGVNDS
                     WLAGLDALAKAPAPWSVLDADRAALATRMQRIAEQVHKQTASTDLAGRARGYGEILVT
                     CAACHAGDRTTAK"
     gene            complement(6909..7625)
                     /locus_tag="VF516_34885"
     CDS             complement(6909..7625)
                     /locus_tag="VF516_34885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112987.1"
                     /translation="MSRPQGGFSLALAVVAGCSTTTSYLYAPQAPSYWSDGYPATTVA
                     VPPEAPQGKVEVASFGITEIKPDGDGPVAALHVRLAVSNDGDATPWTLTPSEQLVEIA
                     GEGRSRPIFVNTDLPTLPAVSIAQHERRVLDLYYPLPTGMREEAALPAFDVLWQLTTP
                     ARSFSSRTRFQRVEQVPPPGHTEVVFWTGWGPYWWYDPFYPDLVFLHHPPMRLHSRPH
                     VIVTRPPRWHSHAVHDHRRS"
     gene            complement(7767..8459)
                     /locus_tag="VF516_34890"
     CDS             complement(7767..8459)
                     /locus_tag="VF516_34890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112988.1"
                     /translation="MKTFTKMLALLVTTPLFAPGLAGADVADPSVATVEVPAPYSYAW
                     HDSRLQSGIGISAMLGVGVGGFTNQTMRDTVSLSVASLWDLRVTIGSHTPLALDLSYV
                     GTASGIDALIGFQHGTLIGTTAEGALRYNILPHNAWNPYTFAGIGWQRYDLTGGSFAL
                     SDTGMNDSDNSIVFPMGAGLAYRDRSGIVADVHGTFRANTRAGLVLDGPGSTSYAPMH
                     TWAASAAFGFEY"
BASE COUNT         1371 a         3286 c         3099 g         1354 t
ORIGIN      
        1 gtctccgcca ccgccaactc gtagatcagc gcgtgcttgc cgtcgcgcgg ccgcagcacc
       61 cggccgatcc gctgcacgta ccggcgcggg ctccccgatc cgccgaccac gatcgccagg
      121 tcggcgtcgg gcacgtcgaa cccctcctcc agcacctgcg acgacaccag caccgggcac
      181 tccccggtcc ggaaccccgc gagcgcccgg gtccgctcgg tgcgccggat ctcgtgcgtg
      241 atcggcatga ccagcagctc gcgcgcgatc gcgtatgccg tcgcgttgtc ggcggtgaac
      301 accagcgcgc ggctgccgcg atgctggccc aggatctggc gcagcgccgc gcgcttgccg
      361 tcgggatacg ccagcagcgc gcggtactcg cgccaggccg ccagcgccgc gcggccctgc
      421 accgtctgca atgcctcgcg cacgaactcc cgccacgcgg catcgggcat cgcgcgctgg
      481 aacgccgcgt agtgcttcgc gaaccgcccg cgcagctcgc gataccgccg ccgctccgcc
      541 ccgtcgagcg ccaccggcac cgtgaccagc tggtggtcgg ccagcccgtc gccccgcagg
      601 tccgccaccg tcagcgcata caccaccggc ccgacgtgcc gcgccagcac cgcccggtcc
      661 tccggcggcg tcgcggtcag cccgagccgc gccggcgcga ccagcatctc gaacacctcg
      721 gtcggacacc acgcccccac gtgatgcgcc tcgtccacga tcaccagccc gaaccgcccg
      781 ccgatccgcg gcgcccacga cgtcgcgctg gcataggtcg ccaccgtcac cggcgcgacc
      841 acatgatcgc cgtcgccgag ccggccgatc ggctgcgccg tgcacaccgc cagcgcccgc
      901 gcccactgat ccagcagcac gcgcgtcggc accaggcaca gcgtcgcgac cccgagccgc
      961 gcgatcgcgg cgatcgccac cagcgtcttg ccggccccgg tcggcagcgc caccaccccg
     1021 cgccccccgg ccgcgagcca gcgctccagc gcgacctcct ggtaccagcg cagccccggc
     1081 atcatccagg tcatcgccac cgcgtggccg cccagctcgt cggacaccgg caccccggcg
     1141 tccgccagct cccggcgcag ggccgcatgg tgctccgccg gcagccgcca cgcgcgccgc
     1201 tcctcgtccc aggccacgcc ggcgatggcg cccggctgct cgccttcgtg ctcggcctcg
     1261 agcaccagcg ttccgcgatc gaatcgaatc cgcatgccgt cgcccctcag caagccgcgt
     1321 accggctcgc cgagatcgcg atccgcgccg cccgccgcgc gtcagccgtg cgaccgatcc
     1381 gcttgctctc cgacgacgat cgcgaagcga cgcggcttca caggcagcgc gggacgcccc
     1441 gctccgcgat cctgcgcctg tccgggtttc gtccggattc cggacggtcg tccgagatcc
     1501 ggccagaatc cggacatcgc agggccgcgc catcgccaag gtccggcgga tgcagcgggt
     1561 ccttcatccg gatcccggtc cctaccgcgg ccgtccctac cgcgcgccgc cgacgcagac
     1621 cgaggtgcgt gcgcggcagg gagcccactc gtcgcgccga atgcgcgact acggcaaccg
     1681 ctgcggagtg agggtgacga tcagccgcgg ccctgccacg gcgcccggag agaagtcccg
     1741 gaacccgtct cgcgcgccga accgcacgcc gcgaaagctc ccatcaccgg acgtctcacg
     1801 cagcatgatt ccgaaattgg gtgtggtgcg gctcgtccag gcccgtacca ggtcggtgac
     1861 atcccagcgg aacacgtcgc cggggacatt gctggcctgg acgatcgtcg cggttgcgag
     1921 cacctgcgga tcgaagtctg gctgggtctg gttgtcgggg tcgccgccgt ctccgttgcc
     1981 gaaccatgcg acccccgagg catcgttcac attcgtgcag ccgaggggca ccacgtcccc
     2041 ctccactccg ttgccctcga gccacggggt ccgcgttccg gatgggatga tgcggaatgc
     2101 gccgatcgtg tacacggacg caacgtcggg caggccgcga tcgaacccga acagcgtcat
     2161 ctcgaggaat gccttctgca cgaacgggaa cgctgtcagg ctcgacagat cgaactgcac
     2221 caggccacgc atcgcgtccg cgctgccgct cgccgagctt ccgatctcga tgctctcttg
     2281 acatccgtag ttgtcattgg agcgatccgc gaacggcgta gcggagaccc accgcgtggc
     2341 cgatgccgcg gtcgatatcg cagtcaggat tggcccgcga gccatcgcca gtagtgcagt
     2401 cgggcccgcc attggccttc acgtagacta ccgacgcctc gggtatgcat gctcccggcc
     2461 ccatcggtgt ggcagctccc tgatcgcaga cgtcgctctg acactggtca tgggcggcgc
     2521 acggctggca tgtgcccgcc acgcatgcca gcccgccggt gcaatccgac tcctgggtgc
     2581 agctcgccgc agtcacggct tgtgcaatcg accccggctc cggtcgctcg cttccgaggc
     2641 caaggtcggg ttggccctgt gcgccgcagc ccagcacgaa caccacgctg atgccccagc
     2701 tgggcatcgt ccagtccgtc cgcttcttca tagacacctc ccggcgagga ggcaaaggca
     2761 aattcgagac caacactttc gccatcctcg ttcccgtgcc gcgcgggagc gcgaatggat
     2821 agcgccgcat cccggcgact cgtgcaccga ctgcgtgcct cgggagcaac ggtaatgtct
     2881 atgctgtcat caggtgctct catcttgacg cagacaacac tcgcatagcc gagatcctcg
     2941 gtgatgcgtg gctacgactg gttcgcgccc agcatatcca gggcatggcc ggtgagatcc
     3001 tgccacagcg acgcgatcgg gcgcggcagc gtgctcacga tatgtcggat ctcgccgggg
     3061 ctgacatgct cgaccagcag gtggatcacc gcgcggagga cgtcgaccgg atccaggcgc
     3121 gggtcgagca tcagctcctt cttgacccgc gcgatcatgt ccgcccggtc gcgcatccgc
     3181 gtcgggtcgt tggcgagggt ccagccctcg tagtagaagc cgcggatcag cgtcggcagc
     3241 tgggcaccga gatcgaccac ctcggctgcc ggcaagcggt cgcggatcgc atgcagtccc
     3301 gcgcgtagcg cgtgcagcgc ccgcggtcgc tcggtcgccg gaagctccag gctctcgccg
     3361 acctcatcca tccacttgtt ggcggtttcc agatgggcat cgaagacgtg tgttcgggtc
     3421 acggtggctc ctcccgtcat cccgttgcac gtagcaaacc aacggccgcc gggcgatcgt
     3481 cgcgctgcaa tggatgagca aactctggtg acccacgttc gcgcgcatct tgcgcgagag
     3541 ctcgatggcc ggagaacgaa ttgcgcctgg ccccgagagc tacgaccagt gacgaacccg
     3601 agaggccgcc gcgggacgtt cggtacctgc agcgaaccgc cggatatcgt ccgcgaagcg
     3661 cagtagccgc gcctcgacca gcgcgttgac gctgcccacc gggaagtgtc cgtcgtgatc
     3721 gcgggcgccg gcgatgcgcc ctgtgacgag ctcgagcgcc tcgtctgccg tggcgacggc
     3781 gtggacatgg aacttgccct cggcgacggc ggcgaccacg tccttgcgca gcatgagatg
     3841 ctgcacgttg ctgcgcggga tcaccacacc gtgctcgccg gtgaggccgc gctgactgca
     3901 gacgtcgaag aacccctcga tcttctcgtt gacaccgccg atcgcttgca gcgcgccgtg
     3961 ctggttgacc gagcccgtca cggcgagcga ctgcttgacc ggcgcgtccg cgatcgccga
     4021 cagcagcgcg cacagctcgg caagcgacgc gctgtcgccc tcgacgccgc cgtacgactg
     4081 ctcgaacacg atcgtggccg acagcgccag cggtacatgc tcggcgtagc gggcgccgag
     4141 cagtccggcg aggatcagca cgcccttgga atggatcgga ccgccgagct cgacctcgcg
     4201 ctcgatatcg accaccttgc cgtccccgat ccgtgcgcgc gcgctgatcc gggtcggatg
     4261 gccgaacgcc tggtcaccga gctcgaccac cgagagcccg ttgatctggc cgaccacctc
     4321 gccgctggtc tcgatcagga cggtgccgcg gcgaactgcc tccagcatgc gctcgcggat
     4381 ccgaccggcg cggtggagct gcgcatcgat cgccgcctgc acatcgccgg cggtcgcggt
     4441 ggatcgcccc gcgtcggtcg cgcagcgctc cgcctcgtgg agcaggtcgc tgatcgaccg
     4501 cacgtgcagc gagagcttgg cgccgtcgct cgccagccgc gacgcgtgct cgatggcccg
     4561 cgcgaccgcg ccgcgatcga acggacgcag gccatgctgg cgaaccaggc cggcgatcag
     4621 ccgggcgtag gcgacgtcgg cgtcgcggct gcggtcgatg tcctgctcga agtcggccat
     4681 caccttgaac aggtcaccga actcgggatc atgggccgcg agcagatagt acagcaagcg
     4741 gtcaccgagc agcacgacct tgatctcgag cgggatgggc tcgggttcca gcgagacggt
     4801 cgacgccagc ccgagcgcct cgcccagcga ctcgatccgg atctcgtggg tgcgcagcgc
     4861 ccgcttgagc gcttcccatg ccagcggctg ttgcagcagc ttgagcgcgt cgagcagcac
     4921 gtatccgccg ttgccgcggt ggagggcgcc gggtcggatc agcgtgaagt cggtgatcag
     4981 cgcgccgagc tccgcgacgt gctcgatccg gccgatcagg ttcgggtggg tcgggttgtc
     5041 ctcgtagatc acgggggcgc cgcgcgccgc ggcgtggtcg accacgacgt tgaccgcgta
     5101 gcggcgaaac cgggcttcgt cgccatggtg atgcaggaca cggcgcagcg cctcggggac
     5161 ctcgtcgtcc gacccgggca ggaaccggcc cgcgccgtcg atcacgtcgt tctcgacctc
     5221 gccgagatac gcgagcaccg cggggagctc gtggtagccg gcgcgcacgg cgtcgaccac
     5281 gcggcgcgcg accgccgcgg cggtcgcgcg atcgagcgcg tggagcgcct ccagctgctc
     5341 gcgggcccac tcgtggaacg tccgcagcag cgcggtgagc tcggcgccga cctgctccat
     5401 cgccgcccgg agctcggcct gctcgtggtc gggcagccgc ccgaacgctt cggcgtcgat
     5461 cgcctcgtca ccggcctcgg tgcggcgcag cgcggcgagc gcgaacccgc catcggtatg
     5521 catcactccg acgcgacgag cgcgggcacg gctctgcagc tcctcgaacg ctgccgcctg
     5581 gcggcgctgg aagtcctggc caagctgctg cttccggttc cggtactcgc cgctgtcgaa
     5641 cgccgccggg atcgcgaccc gcagctcggc gaccgcgtgc tccatgttgg cgcgcaagcg
     5701 agtcgccgcg ccggcgggca gccggagtgc gcgtggccgg cgcggctcga cgaagttgtt
     5761 gacgtagcac cagtcgacgg gagcggcgtt ccgcgcagcc tcgcgctcca gcatctggcg
     5821 cagcgtcgtg tgcttgccga cacccgccgg cccgagggcg aacaggttgt agccgtcatg
     5881 ccggatcccg accgcgacac ggacggcttc gatggcgcgc gcctgtccca ggacctggat
     5941 cggctctggc tcgtcagcgg tggagtcgaa cgggatgtca cgcggatcac agctccgaaa
     6001 tagctcgtcg gggcgcagct cggtgggcat gcgagggagg gtttgcggat cgcgtgccgt
     6061 gcccccgggc gcgccgaggc ccaggtgcgc gcagatcttg cgagcaaccg gctcgcatcg
     6121 accgagtttg ggcggccgag acgtggcggg cagcgaatcg tcctggaatg acggatgcag
     6181 cgatgaggcg tcatgcgcgt ctccgctttc ctgatcctcg cgcttgccgc ctgcgaaccg
     6241 agtcatgcga gccccgccgt gcccggcgat cacgaggcca cggtccggtc tcacatgcgc
     6301 gagcactacg atctgttcag cgccatccag cacgtcgtca tccgcaatga cctggacacg
     6361 acccgagcgc tcgcgcgatc gatcggccac gcaccggccg gtcctgggct cgatcgctgg
     6421 gcgacccagg ccgcgcttgc ccgtgcgcgg gccgccgagc ttgcggcggc gccgggaacc
     6481 gacgaggcct gccgccgcac cgcgaggctc gccgaggcct gtgcccggtg ccacatcgat
     6541 gccggcgtcg tcccgatgtt cggctcgccg cccccgctgc ccggtgaggc ggcggacaag
     6601 gatcgcatgg cgctgcacct gtgggccgcc gagcggctgt ccgaggggat gatcgggggt
     6661 gtgaacgatt cgtggctggc cggtctcgat gcgctggcga aggcgccggc gccatggtcg
     6721 gtgctcgacg ccgaccgggc cgcgctggcg acgcgcatgc agcggatcgc cgaacaggtg
     6781 cacaagcaga cggcgagcac cgacctcgcc ggccgggcac gtggctacgg ggagatcctc
     6841 gtcacctgcg cggcctgtca cgcgggcgat cgcacgaccg cgaagtgatc gcggccggcg
     6901 gcccgacgtc acgatcgccg gtgatcgtgg accgcgtggg agtgccagcg cggcggccgc
     6961 gtgacgatga cgtgcgggcg cgaatggagg cgcatcggag ggtgatggag gaagacgagg
     7021 tcgggataga acgggtcgta ccaccagtac ggtccccagc cggtccagaa cacgacctcg
     7081 gtatggccgg gcggcggtac ctgctcgacg cgctggaacc gcgtgcgcga cgagaacgac
     7141 cgcgccggtg tggtcagctg ccacagtacg tcgaacgcgg gcagcgcggc ctcctcgcgc
     7201 atgccggtcg gcagcgggta atacaggtcg agcacgcgcc gctcgtgctg ggcgatgctg
     7261 acggccggca gtgtcgggag atctgtgttg acgaagatcg gccggctgcg accctctccg
     7321 gcgatctcga cgagctgctc cgatggggtc agggtccacg gcgtcgcgtc accgtcattg
     7381 gagactgcca gccggacatg cagtgcggcg accgggccgt cgccgtccgg cttgatctcg
     7441 gtgatgccga acgaggcgac ctcgaccttg ccctggggcg cctcgggggg caccgcgacg
     7501 gtggtcgcgg gataaccgtc ggaccagtag ctgggggcct gcggtgcata gagatagctc
     7561 gtcgtggtcg agcagcccgc gacgacggcg agcgcgaggg agaatccgcc ttgtggtctg
     7621 gacatgctgc catcgaaagc atcaggcatg cccgcaccgg gtacccgccg cgttgccccc
     7681 gtcccggtct gcaagtcgat ccgctggaac gcaagtcatt cgcgattcgc ccgcgcgatc
     7741 ggcttgcagc gcacgccatg ccgtcatcag tactcgaagc cgaaggcggc gctggccgcc
     7801 caggtgtgca tcggtgcata gctggtggag ccgggaccgt cgagcaccag ccccgcccgg
     7861 gtgttcgcgc ggaacgtgcc gtgtacgtcg gccacgatgc cgctccggtc gcggtacgcc
     7921 aggcccgcgc ccatcgggaa cacgatcgag ttgtcactgt cgttcatccc ggtgtccgac
     7981 agggcgaagc tgccgcccgt gaggtcgtag cgttgccagc cgataccggc gaaggtatac
     8041 gggttccacg cgttgtgcgg caggatgttg tagcggagcg caccctcggc agtcgtgccg
     8101 atcagcgtgc catgctggaa gccgatcagc gcgtcgatgc cgctggcagt tccgacatag
     8161 ctgaggtcga gcgcgagcgg ggtgtgtgaa ccgatcgtga cgcgcagatc ccacaggctg
     8221 gccacgctca acgacaccgt gtcgcgcatg gtctggttgg tgaacccgcc gaccccgacg
     8281 ccgagcatcg cgctgatgcc gatgcccgat tgcagccggc tgtcatgcca cgcgtagctg
     8341 tacggggccg gcacctcgac ggtcgcgacg ctggggtcgg cgacgtctgc gccggcgagg
     8401 cctggcgcaa acaggggagt ggtgacgagc agcgcgagca tcttcgtgaa ggtcttcatc
     8461 gagcttctcc ttctcggtcc acctcgcatg cgagcattgg atgtgccagc cggagcgtcg
     8521 gttcgccccg cggttcgcgc acaactggcg cacgatcgaa gtgcgcgagt aaggcattcg
     8581 cgaatcccct gctctgagat gtcggctcgc gagcttgcgg agcattgcac ccaggccgtg
     8641 cacggccgga cagcagtcct ccgtcggcga cgagcccgcg gtcgctccgc ggccgaccgg
     8701 gttccgctcg gtgctggtct agatcacagg gcggccttgc gcctcgacac gcatgcgcgc
     8761 gctccgcaca ttcgggacgc tggccgcgag catcacgccg acgcattcac gaactgagtg
     8821 cggactcggc ttcgcggccg atcggatcag acatcgtgcg cgaacacgcg cagatgactc
     8881 cctcacgcgc atgctgcagc gaaccgcagc acgccatgat tgcgcaaaac cgcggctcac
     8941 cacagcgccg ctgtccttgg cgcacgcgcc atgagacacc ctgtgtcaac ctcgttctct
     9001 tcaagcggcc tcctggtcga tggagtgagg agtaagctga gacgcggcac ggtgtttggc
     9061 cgggtcgtag gcgacgcggt tctgccagca gtgccagatc acccggcacc
//
LOCUS       DASVOR010001882         1888 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340702,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001882 DASVOR010000000
VERSION     DASVOR010001882.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340702"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..452
                     /locus_tag="VF516_34895"
                     /pseudo
     CDS             39..452
                     /locus_tag="VF516_34895"
                     /inference="COORDINATES: protein motif:HMM:NF013050.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(585..1433)
                     /locus_tag="VF516_34900"
     CDS             complement(585..1433)
                     /locus_tag="VF516_34900"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8112989.1"
                     /translation="MQSRKLPLATGLTYHVLEWDGPGDLTFVLVHGFSDLGYGWSEVA
                     PRLAQHGHVIAPDLRGHGDSDWVGPGGYYHYMDYVADLDDVIRQLARRRVVLVGHSMG
                     GSVCSYHAGTRPERPIALALLEGLGPVDLTDVDGPARTAAWIDAWRAARSKVRPMASL
                     DEAVHRLRRNDDMLGEARARQLAEVGTRPVDGGLVWKHDPLHMTFGPYPFRLQTAIKY
                     WQRITCPVLIIDGADSRLTLPADERARRRTHFARHRHVVIDGAGHMIPRHQPARVAEL
                     ILELVG"
     gene            complement(1472..>1888)
                     /locus_tag="VF516_34905"
     CDS             complement(1472..>1888)
                     /locus_tag="VF516_34905"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEX8112990.1"
                     /translation="GATATGARAVGPSWMDPLMTFNADTCGSDVLAQVGIGNAFGDRL
                     RLYPLAADLGKTAPRDAAGRDVRYPRVTLDEVAARRPDLVILPDEPHAFTEADEAVLR
                     GALPGVRVVRICGKDLFWYGAWTIDALDRLAVQLAA"
BASE COUNT          232 a          658 c          716 g          282 t
ORIGIN      
        1 tcgccccgct gttcgggctg gtcgcggtgt tctggttcgt gtacgaccag agcgcgtcga
       61 cctggatctt cttcgcgcgc gacttcgcgg atctcgatct cggcggctcg ctctcgatca
      121 cgcccgatca gatccagggc accaacccgg tgttcatcct gctgctcacg ccgatcttca
      181 accggctgtg gggctggctc cgcgaccgcc gcgggcgcgc ggtgcccgac acccgcaaga
      241 tgcggtacgg gttctggatc gtcgtcctgg cgatggcggg catggcggca gccggggcga
      301 gcgcggcgtc cgggaaggtc agcgcctggt ggctcctggc ggcgacgttc gtgatcacgc
      361 tcgccgagct gtgcatcaac gtggtcgggc tggagctgtc ctaccgccgc gcgctgcccg
      421 ggaccaggag cgccgtgacc ggggcgttcc tggccacggt gttcgtcggc gatctgttcg
      481 gcgggatgtt cgcgcagctg tacgggcgga tctcccccgg ggcgtacttc ctggtccagg
      541 cggtgatcgc ggccgcggcg gcgctggtgt tccgccgggt cagctcagcc gacgagctcg
      601 aggatcagct cggcgacgcg ggccggctgg tggcgcggga tcatgtggcc ggcgccgtcg
      661 atcacgacgt ggcggtggcg ggcgaagtgc gtccggcggc gcgcacgctc gtcggcgggt
      721 agcgtgagcc ggctgtccgc gccgtcgatg atcagcaccg ggcaggtgat ccgctgccag
      781 tacttgatcg cggtctggag ccggaacggg tacgggccga acgtcatgtg cagcggatcg
      841 tgcttccaca ccaggccgcc gtcgaccggc cgggtcccga cctcggcgag ctggcgcgcg
      901 cgcgcctcgc cgagcatgtc gtcgttcctg cgcagccggt gaaccgcctc gtcgagcgag
      961 gccatcggcc ggaccttgga ccgcgcggcc cgccaggcgt cgatccacgc cgcggtgcgg
     1021 gccgggccgt cgacgtcggt caggtcgacc gggcccagtc cctcgagcag cgccagcgcg
     1081 atcggccgct cgggccgggt gccggcgtgg tacgagcaca cgctgccgcc catcgagtgg
     1141 ccgaccagca cgacgcgccg gcgcgccagc tggcggatca cgtcgtcgag gtcggcgacg
     1201 tagtccatgt agtggtagta gccgccgggg ccgacccagt cgctgtcgcc gtggccgcgc
     1261 aggtccggcg cgatgacgtg gccgtgctgc gcgagccgcg gcgcgacctc gctccagccg
     1321 tagccgagat cgctgaagcc gtggaccagg acgaacgtga ggtcgcccgg gccgtcccat
     1381 tcaaggacgt ggtaggtgag gccggtcgcg agcgggagct tgcgggactg catgcggccg
     1441 ccatcatcgc tcaccgccgc tcgattgtcg ctcaggccgc gagctggacc gcgagccgat
     1501 cgagcgcatc gatcgtccac gcgccgtacc agaacaggtc cttgccgcag atccgcacga
     1561 cccggacgcc gggcagcgcg ccgcgcagca cggcctcgtc ggcctcggtg aacgcgtggg
     1621 gctcgtcggg gaggatcacg agatcgggcc tgcgcgcggc gacctcgtcg agcgtgaccc
     1681 gcgggtagcg cacgtcgcgg ccggcggcgt cgcgcggcgc ggtcttgccg aggtcggcgg
     1741 cgagcgggta cagccgcagg cggtcgccga acgcgttgcc gatcccgacc tgggcgagca
     1801 cgtccgagcc acaggtgtcg gcgttgaacg tcatcagcgg atccatccag ctcggcccga
     1861 ccgcgcgcgc cccggtggcg gtcgcgcc
//
LOCUS       DASVOR010001883         2102 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_136610,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001883 DASVOR010000000
VERSION     DASVOR010001883.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_136610"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..710
                     /locus_tag="VF516_34910"
     CDS             <1..710
                     /locus_tag="VF516_34910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HEX8112991.1"
                     /translation="SFTNTGSGNLEDTVTANNLEACDEVARQLRLRNIGGIIVVDFVD
                     MDKEGNRRKVWDAFQKALARDRSRTNVTKISELGLVEMTRKRTRESLVQLLTEPCPTC
                     EGAGVVKSVTTVAYEILREVRRSGTLVDSDKIEIECAPRVAELLSKEERDYLDHLEKR
                     FQKLITVVGQKGWKPDQFRVAGKMSTDLAAAAAAEAPKNGHSQGKPRSAGSSSGGGRS
                     GRRRRGGRGRGRANASA"
     gene            complement(1040..1531)
                     /locus_tag="VF516_34915"
     CDS             complement(1040..1531)
                     /locus_tag="VF516_34915"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEX8112992.1"
                     /translation="MTHRIRIARTEHKFSCAHMTVFADGTKERLHGHNYTVAVAIEVE
                     RVDVATMIPFAPIKAAIAELCAAWKERVLLAAGNPWFEIIGGSDGDELEFRLCGARYV
                     MPRGDALLLPIDNISVEALAAHAAALLRDKLLALGLPHALALEVAIEESPGQGASCAL
                     PLR"
     gene            complement(1852..>2102)
                     /locus_tag="VF516_34920"
     CDS             complement(1852..>2102)
                     /locus_tag="VF516_34920"
                     /inference="COORDINATES: protein motif:HMM:NF040267.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5942 domain-containing protein"
                     /protein_id="HEX8112993.1"
                     /translation="GHGNALFFSALVPLALLVVGYGAPRLRAMLAGVAVGVAAHLAFF
                     AAVPLAAVHVPSFIGHSTLWLIGNALACLGIARLALRR"
BASE COUNT          330 a          776 c          740 g          256 t
ORIGIN      
        1 gcagcttcac caacaccggc tcgggcaacc tcgaggacac ggtcaccgcc aacaacctcg
       61 aggcctgcga cgaggtcgcg cgccagctcc ggctgcgcaa catcggcggc atcatcgtcg
      121 tcgacttcgt cgacatggac aaggaaggca accgccgcaa ggtgtgggac gcgttccaga
      181 aggcgctcgc ccgcgaccgc tcgcgcacca acgtcaccaa gatcagcgag ctcggcctgg
      241 tcgagatgac ccgcaagcgg acccgcgagt cgctggtcca gctgctcacc gagccgtgtc
      301 cgacctgcga gggcgccggc gtcgtcaagt cggtgaccac cgtcgcctac gagatcctgc
      361 gcgaggtccg ccgctccggc accctggtcg acagcgacaa gatcgagatc gagtgcgccc
      421 cgcgggtcgc cgagctcttg tccaaggagg agcgcgacta cctcgaccac ctggagaagc
      481 ggttccagaa gctgatcacc gtcgtcggcc agaagggctg gaagccggat cagttccggg
      541 tcgccggcaa gatgtcgacc gacctcgcgg ccgcggccgc cgccgaggca ccgaagaacg
      601 gccacagcca aggcaagccc cggtctgccg gctcgtcgtc gggcggcggg cgcagcggcc
      661 gtcgccgccg aggcggtcgg ggccgcggcc gcgccaacgc cagcgcctag cacgcaggga
      721 acacgtacgc gctcgctccc ggcttacagg aggacagcac acgctcgccc ccggcgcgca
      781 tgagaacgcg cacacgctcg ctcgccgggg gctgctgcgc ggatgggtgt cgggaccgcg
      841 cccgccgaat caccccccgt cctgcgcgga ccccgctagg tatgtgcggg ggcaacgaac
      901 ggccaatcgt aatcacgatc gcggcgcccg accccatcct gctcgcggtc gcccccgggc
      961 ggcttcgcgt tgacgtccgg cactggccct gagagaagcc ttccccgtcc cactcacgcc
     1021 gggatcgctg acttcagggt cagcgcaacg gcagcgcgca gctcgcgccc tggcccgggc
     1081 tctcctcgat cgcgacctcc agggcgagcg cgtgcggcag cccgagcgcg aggagcttgt
     1141 cgcgcagcag cgcggcggcg tgcgcggcga gcgcctcgac cgagatgttg tcgatcggca
     1201 gaagcagcgc gtcgccgcgc ggcatcacgt aacgcgcgcc gcacagccgg aactcgagct
     1261 cgtcgccgtc gctgccgccg atgatctcga accacgggtt gccggcggcg agcagcacgc
     1321 gctccttcca cgccgcgcac agctcggcga tcgccgcctt gatcggcgcg aacgggatca
     1381 tcgtcgcgac atcgacgcgc tcgacctcga tcgcgaccgc gacggtgtaa ttgtggccgt
     1441 gcagccgctc cttcgtgccg tcggcgaaca ccgtcatgtg cgcgcacgag aatttgtgct
     1501 cggtgcgcgc gatcctgatc cggtgggtca cggccagagt gctatcacaa cccactgcgc
     1561 ggggcgcagg gcgcggtcgg agtgcccggt cgcggtcggg gtgcccggtc gcggtcgggg
     1621 tgcgatcgcg cgacgtcaac gcacgctcgc cgggggcgac cgcgagcagg atggggtcgg
     1681 gcgccgcgac cgtgattacg attggccgtt cgttgccccc gcacatacct agcggggtcc
     1741 gcgcaggacg gggggtgatt cggcgggcgc ggtcccgaca cccatccgcg cagcagcccc
     1801 cggcgagcgt gcgtgtccgc gctctcccgg gcgccacgcc cgacccaccc cctaccggcg
     1861 cagggcgagg cgcgcgatgc cgaggcaggc cagcgcgttg ccgatcagcc acagcgtgct
     1921 gtggccgatg aacgacggca cgtggaccgc cgcgagcggc accgcggcga agaacgccag
     1981 gtgggccgcg acgccgaccg ccacgccggc cagcatcgcg cgcagccggg gcgcgccgta
     2041 gccgaccacc agcagcgcga gcgggacgag cgcgctgaag aacagcgcat tgccgtggcc
     2101 gg
//
LOCUS       DASVOR010001884         3707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_20898,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001884 DASVOR010000000
VERSION     DASVOR010001884.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20898"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="VF516_34925"
     CDS             <1..459
                     /locus_tag="VF516_34925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0120153 - calcium-dependent carbohydrate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lectin domain-containing protein"
                     /protein_id="HEX8112994.1"
                     /translation="VTVTGTSGGLTHSKTITWTITAGTGTSCPASYTLKFNGHSYRAT
                     SVDTYANVAASCHADGEHVVVINDSSENTWALGQLTSSNGFVWIGLHFTAGAWTWDDG
                     TLLGSGFEAFSGAPPTSPSSPCVDANKTDGSWATFSCTATHPTLCECDGP"
     gene            complement(518..748)
                     /locus_tag="VF516_34930"
     CDS             complement(518..748)
                     /locus_tag="VF516_34930"
                     /inference="COORDINATES: protein motif:HMM:NF022829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HEX8112995.1"
                     /translation="MARAVSAADVEKALAGIDFPKTKEEVVEYAAERLPPDSPVLDAI
                     RKLPEGVYRTASDIALGFGEEKQEERAERRGR"
     gene            complement(771..1577)
                     /locus_tag="VF516_34935"
     CDS             complement(771..1577)
                     /locus_tag="VF516_34935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112996.1"
                     /translation="MATQPSTVETLEEGDLFFFYRPRIGTDQVRGWRDVQRFYMVLAA
                     RTPLRIYRLFVVGRKKLPQLVRGQHPDRRNWAAVVLVSDDPADLRRELAAYEYDTRTR
                     GRRFVPAANPLGEGRYQLVRHRDHSELVYALELPRHIGPAQREFEIHQEASYIVAVKN
                     PEITIPGTPSTKYPPAYPERLRRKFGRQRWIDAEPDLLDYENAQLLLLAAHSEEVEDE
                     LGVHIDTEDERLSTAKVCRELRVSCERNKVTPLLQGTFPEHEQNEVTYGK"
     gene            complement(2249..3118)
                     /locus_tag="VF516_34940"
     CDS             complement(2249..3118)
                     /locus_tag="VF516_34940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112997.1"
                     /translation="MTHARSFQILSASTALMVSACAADESPSDSADAADGTVVHMLRV
                     RNPNSARIVSDAITPRIAGLTYFGGPLLNHINVHPVFWNSATKLQSNLNAFYPAIVGG
                     SPLWAVLAQYNIGSGTAFNGIVDTNVTRNVSDATVQSRISALITAGRLPAPTANTYYP
                     VHFPSGMTITASDGSQSCVVFCEYHSTFVRSGVNVNYGVIPDLAQAGCNGGCGGSTVT
                     NNTDAIATHVLAEAATDPAIGLATVFGPPLAWYDQVDNDEIADLCNGIQGSSAGFTVQ
                     KLWSNAAHACVDH"
     gene            3335..>3707
                     /locus_tag="VF516_34945"
     CDS             3335..>3707
                     /locus_tag="VF516_34945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8112998.1"
                     /translation="MRSLLLLLCSPVVVAVLAWTAFAACIDAPPEPRRSPARLVVAWD
                     PLACGDPHRVVVELQDDTGATTSASTPCSLGTLAVDVAHFGSYRGRISAWALDPPARP
                     TAPVDRTIDLTIDQPIVRWDVM"
BASE COUNT          563 a         1246 c         1268 g          630 t
ORIGIN      
        1 gtgacggtga ccggcaccag cggcgggctg acccacagca agaccatcac ctggacgatc
       61 accgctggca ccggaaccag ctgcccggcg agctacacgc tgaagttcaa cggccactcc
      121 taccgggcga cgtcggtgga cacctacgcc aacgtggccg cgagctgcca cgcggacggc
      181 gagcacgtcg tcgtgatcaa cgacagcagc gagaacacct gggcgctggg ccagctgacc
      241 tcgtccaacg ggttcgtctg gatcgggctc cacttcacgg cgggcgcctg gacctgggac
      301 gatgggacac tgctcggctc cggcttcgag gcgttctccg gcgctccgcc caccagcccg
      361 tcgagcccgt gcgtcgacgc caacaagacc gacgggtcgt gggccacctt cagctgcacc
      421 gccacccatc cgacgctgtg cgagtgcgac gggccctgag ggagccgtcg cactccgcgc
      481 cgaccggggt cagcgcgcgc tcgcggaggg acgaggacta gcgccctcgg cgctccgcgc
      541 gctcctcttg cttctcctcg ccgaacccca gcgcgatgtc cgacgccgtg cgatagaccc
      601 cttcgggcag cttgcggatc gcgtccagca ccggcgaatc cggcggcagc cgctcggcgg
      661 catattcgac cacctcttcc ttggtcttcg gaaagtcgat gcccgcaagg gccttctcga
      721 cgtcggcggc gctcacggcg cgcgccatcg gctcactctc cacatctcgt tcatttgcca
      781 taagtcacct cattttgttc atgttcggga aaggtcccct ggagcagggg cgtgaccttg
      841 ttgcgctcgc agctgacgcg gagctcgcgg cagaccttcg cggtgctcag ccgctcgtcc
      901 tcggtgtcga tgtggacgcc gagctcgtcc tcgacctcct cgctgtgcgc tgcgagcagg
      961 agcagctgcg cgttctcgta atcgagcaga tccggttcgg cgtcgatcca gcgctggcgt
     1021 ccgaacttcc ggcgcaggcg ctcgggatag gccggcgggt acttcgtcga aggcgtcccc
     1081 gggatcgtga tctcggggtt cttgaccgcg acgatgtagc tcgcctcctg gtggatctcg
     1141 aattctcgct gtgcgggccc gatgtgtcgc ggtagctcga gcgcatacac cagctcgctg
     1201 tgatcgcggt ggcgcaccag ctgatagcgg ccctcaccga gcgggttggc cgccggaacg
     1261 aaccgcctgc cgcgcgtccg ggtgtcgtac tcgtaggccg cgagctcgcg ccggaggtcc
     1321 gccggatcgt ccgataccag gacgacggcg gcccagttgc gccgatccgg atgttgcccg
     1381 cgcaccagct gcggcagctt cttccggccg acgacgaaca accgatagat ccgcagcggc
     1441 gtcctggccg cgagcaccat gtagaaccgc tggacgtccc gccagccgcg gacctggtcg
     1501 gtcccgatgc gcggccggta gaagaagaac agatcgcctt cttcgagcgt ctcgacggtg
     1561 cttggctgcg tcgccattgc gcagaatcaa tgcagcacgt gagccaatcg agctcgcggg
     1621 tgcgtaggtg aactccgcgg ccggctcatt ccgcggcctt ggcggcgatc cggatctgga
     1681 catcctccgc atgcaaccgc gatgaacgtc tccgatcacg agaatgtatt cgaccggcac
     1741 gggcgcgccg ggcctcggct cggacggagt cgcagcctgc ggcgcggcga cgcgatccct
     1801 gactctctcc attcgaggat attccatatt tgtctcgcca tgtcgccgtg tcatcgccca
     1861 ggaaagctgt cattgtgagc gccgagcgca cgcgtgatcg gcaggtgccg cacgatcaca
     1921 cggcgcacgc accgcggtcc gggaagaccc gagcggcgcc gcgacggagt ctggctccag
     1981 aggtcttcgg gtcaccgggc cggcgagagg gcgcgagcgc cgtgctcaca gtcctctggc
     2041 cgccaacacc agcccgagca tcgcggcgcg cccggcggga ggcttcgacc tggtgttcca
     2101 ggcgaacacc ggatcgctgt ggaccgcggg gtcgaccggc accggcgacc tgcggctcgg
     2161 gatggcatcg cgcacgagcc cgtccgccaa ctgacggagg gctcgagccc gggtagttgc
     2221 acccgccctc accgatctcg acctacgatc agtggtccac gcaggcgtgg gccgcgttcg
     2281 accacaactt ctgcacggtg aagccagcgg acgaaccctg aatgccgtta cagagatcgg
     2341 cgatctcgtc gttgtccacc tggtcgtacc aggcgagcgg cgggccgaac accgtcgcga
     2401 ggccgatggc gggatcggtg gccgcctcgg cgagcacgtg cgtcgcgatc gcgtcggtgt
     2461 tgttggtgac ggtgctgccg ccgcaaccac cgttgcagcc ggcctgggcg aggtccggga
     2521 tgacgccgta gttgacgttg acgccgctgc ggacgaacgt gctgtggtac tcacagaata
     2581 ccacgcagga ctggctaccg tccgatgctg tgatggtcat gcccgacggg aaatgcaccg
     2641 ggtagtaggt gttggcggtc ggcgccggca gcctcccggc cgtgatcagg gcgctgatcc
     2701 gcgactggac cgtggcgtcc gacacgttgc gggtgacgtt ggtgtcgacg atcccgttga
     2761 aggcagtccc ggacccgatg ttgtactgcg cgagcacggc ccacagcgga ctgccgccga
     2821 cgatcgcggg atagaaagcg ttgaggttgg attgcaactt tgtcgccgag ttccagaata
     2881 ctggatgaac gttgatatgg ttgagcagcg gcccgccgaa gtaggtcaaa ccggcgatgc
     2941 gcggcgtgat ggcgtcggac acgatgcggg cgctgttcgg gttacgcacc cgtaacatgt
     3001 gcacgacggt gccgtcggcc gcgtcggcgc tgtcgctggg gctctcgtcg gccgcgcagg
     3061 ccgagaccat cagagcggtt gatgcggaca ggatctggaa cgagcgagca tgcgtcatgg
     3121 cagcgaccct gtcacggatt gcgtctctcc gcgtacctcg ttttctggag cattttacgg
     3181 cctgcagctc gacggtcagc cgctgggcgg tctgacgttg tccggattct ggccggatct
     3241 cggacgaccg gccggatccc ggacgaaatc cggacagccc ggcgggtgat tttcagcgct
     3301 tgcgcgtggc acgcggcatg cgaagcgccg gtgcatgcgc tcgttgctcc tgctcctgtg
     3361 ttctcctgtg gtcgttgccg tgctggcctg gaccgcgttc gcggcgtgca tcgacgcgcc
     3421 gcccgagccg cggcgatcgc cggcacggct ggtggtggcg tgggatccgc tggcgtgcgg
     3481 cgatccgcac cgggtcgtcg tcgagctgca ggatgacacc ggcgcgacga catcggcgtc
     3541 gacgccgtgc agcctcggca cgctcgcggt cgacgtcgcg cacttcggca gctaccgcgg
     3601 gcggatctcc gcctgggcgc tcgacccgcc ggcgcgtccg accgcgccgg tcgatcggac
     3661 gatcgatctg acgatcgacc agccgatcgt gcgctgggac gtgatgg
//
LOCUS       DASVOR010001885         3098 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_49394,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001885 DASVOR010000000
VERSION     DASVOR010001885.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49394"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VF516_34950"
     CDS             complement(<1..379)
                     /locus_tag="VF516_34950"
                     /inference="COORDINATES: protein motif:HMM:NF019984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HEX8112999.1"
                     /translation="MADELRRKLGLVLSEEKTLVTPVTSTMRFLGHHINVRGQPDDGR
                     FVPRVVIPKERSKQLRHKIKNLFRRSTCGRTLESLLRLANPILRGWANFYRHAWGAKQ
                     VFCANDHYVWWTILRWLRKKHPQT"
     gene            complement(525..1811)
                     /gene="ltrA"
                     /locus_tag="VF516_34955"
     CDS             complement(525..1811)
                     /gene="ltrA"
                     /locus_tag="VF516_34955"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019332377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX8113000.1"
                     /translation="MGLVNQRWEEPVPEAKPYVIPKQLVWDAYRRVKANQGAAGVDGE
                     TLAMFEQDLRGNLYKLWNRMSSGSYFPPAVRLVEIPKESGGTRPLGIPTVADRIAQTV
                     VKMVLEPLVEPIFHGDSYGYRPGKSALDAIEVARKRCWRADWVIDLDIKSFFDCLDHD
                     LVERAVAKHTELPWVRLYVARWLRAPLQRPDGTEEPRTRGTPQGGVISPLLANLFLHY
                     AFDAWMSRTFPVTPFERYADDAIVHCRSEGHAKKVLDAIRDRLKQCGLELHPVKTRTI
                     YCKDDDRRGDSESISFDFLGYTFQPRRAKNRSGKYFVSFLPAISAKAAKSIRQTIRRW
                     RMAATRNNQRLEDLARALNPTMWGWANYYGRFYRSKFLQVLQHFNGALASWVRRKYKR
                     FRRRERASLRWLGRIARREPKLFVPWQLGLKPEAGR"
     gene            complement(1911..>3098)
                     /locus_tag="VF516_34960"
     CDS             complement(1911..>3098)
                     /locus_tag="VF516_34960"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HEX8113001.1"
                     /translation="PRGRSPEDNVRRLQGRLRDVAKQQPGRKFHALYDRIYRRDVLYE
                     AWNQVRRNQGAAGVDAQTIAAIEQDGVERFLEAIQAELRENAYRPQAVLRWYIPKADG
                     KQRPLGIPTVRDRVVQAAAKLVLEPIFEVGFRDCSYGFRPRRSTTQALEKIRVTVNRG
                     FNHVLDGDIRDFFGQIDHSVCLSLVARRISDRRVLKLVRQWLEARVMEDGRETTKLSG
                     APQGGVISPLLSNIYLGFLDDVWSKRCADLGVLVRYCDDCAPRRRGKETARSNGSSCA
                     AREMGAGPSGSGCRTRAQTTGCCSGPMTGVVSETEKAGPTRQVCAVKTNASEPSTTCR
                     KRRDVHRNRAPVVGSGQPRGVPADCSRDDRHEDGVSMAQALVRNVGTFRLDAKRELQA
                     EDP"
BASE COUNT          500 a         1090 c          905 g          603 t
ORIGIN      
        1 gagtctgggg atgcttcttg cggagccatc gcaggatggt ccaccacacg tagtggtcgt
       61 tggcgcagaa gacttgcttc gcgccccacg cgtgtcgata gaagttcgcc caaccccgca
      121 ggatggggtt cgccaagcgc aacaggctct caagagttct gccgcaggtt gacctgcgaa
      181 agagattctt gatcttatgg cgcagctgct tcgagcgctc tttcgggatc accaccctcg
      241 gcacgaaacg accgtcatcc ggctgtccgc gcacgttgat gtggtgcccg agaaagcgca
      301 tcgtgctcgt caccggcgtg acgagcgtct tctcctcgga gagaacgagc ccgagcttgc
      361 gcctgagttc gtccgccaag gctgccttct cggcttcggc taccgcacga gtctcctgag
      421 gatccccgcc cgttgccacg agcacgatga gtcgagtagc ccggggggat cgcaccccca
      481 ggccctcaca gaaccggacg tgaaactctc gcctcatccg gctcctacct tccagcctcg
      541 ggtttcaggc cgagctgcca cgggacaaac agcttgggct cccgtcgcgc gatgcgcccc
      601 aaccagcgca acgacgctcg ttctcgacga cgaaaccgct tgtacttccg tcgcacccag
      661 gatgccaggg ccccgttgaa gtgctgcagg acctggagga acttcgaccg gtaaaaccgt
      721 ccgtagtagt tcgcccagcc ccacatcgtc gggttgagtg ctcgagccag atcttccagg
      781 cgttggttgt tcctggtcgc tgccatccgc catcggcgga tggtctgccg gatcgacttg
      841 gcggccttgg cgctgatcgc aggcaggaag ctcacgaagt acttcccgct gcgattcttc
      901 gcccgtcgag gctggaaggt gtagccgagg aagtcgaacg agatgctctc gctgtctccc
      961 cgtcgatcgt cgtccttgca gtagatggtc ctggtcttca ccgggtggag ttccagtccg
     1021 cactgcttga ggcgatcgcg gatcgcgtcg agcaccttct tggcgtggcc ctcgctcctg
     1081 cagtggacga tggcatcgtc ggcatagcgc tcgaacgggg tcaccggaaa cgtccggctc
     1141 atccacgcat cgaacgcgta gtgcaggaac aggttggcga gcagcgggct gatgaccccg
     1201 ccttgcggcg tgcccctcgt ccgcggctcc tcggtaccgt ctggccgctg cagcggggca
     1261 cggagccagc gcgcaacgta cagccggacc cacggcagtt cggtgtgctt cgcgacggcg
     1321 cgctcgacga ggtcatgatc gagacagtcg aagaacgact tgatgtccag atcgatcacc
     1381 cagtcggccc gccagcaccg tttcctcgcg acctcgatgg catcgagcgc cgacttgccg
     1441 ggtcgatagc cgtaggagtc cccatggaag atcggctcca ccaagggttc caggaccatc
     1501 ttcacgaccg tctgcgcgat ccgatccgcc accgtcggaa tcccgagggg tcgggtgcct
     1561 ccgctttcct tcggtatctc gacgagccgc accgctggcg gaaagtacga gcccgacgac
     1621 atccggttcc agagcttgta caggttgccc cgcaagtcct gctcgaacat cgccagcgtc
     1681 tcaccatcca cgcccgcggc gccctggttc gccttcaccc gtcggtacgc atcccatacg
     1741 agttgcttcg gaatcacgta aggctttgct tcaggcaccg gttcctccca tcgctggttg
     1801 accagtccca tcggccagaa agttgggccc cgtcgctccg cccgcgttac caggcttcat
     1861 cgctacaacg ggcccatccg tcccttcgcc tcgcatcgat actcggctcc tcacgggtcc
     1921 tccgcttgga gttctctctt tgcatcgagg cggaaggttc ccacgttccg cacaagagcc
     1981 tgcgccatgc tcacgccgtc ttcatgccgg tcatcacgcg agcagtcggc aggcacccct
     2041 cgcggttgtc ccgagccaac gactggagct cggtttcgat ggacgtccct acgctttcga
     2101 cacgtcgtcg acggttcact tgcgttcgtc ttcacggcgc acacctgacg ggttggtccc
     2161 gccttttccg tctcgctcac caccccggtc attggaccgg agcagcagcc ggtggtttga
     2221 gccctggtcc tgcaacccga gcccgagggg cctgccccca tctctcgtgc agcacagctg
     2281 cttccattcg accttgcggt ctcctttccg cgccttcgtg gcgcacagtc gtcgcagtag
     2341 cgcaccagca cgcccagatc ggcgcaccgt ttgctccaca cgtcgtcgag aaaccccagg
     2401 tagatgttcg acaacagcgg cgagatcacc ccgccctgcg gcgcaccgct gagcttggtc
     2461 gtctcgcgcc cgtcttccat cacccgcgct tcgagccact gtctgaccag cttcaggacc
     2521 cgacgatccg agatcctgcg ggccaccagc gacaggcaca ccgagtgatc gatctgcccg
     2581 aagaagtcgc ggatgtcccc gtccagcaca tggttgaagc cccggttcac ggtcacacgg
     2641 atcttctcca gcgcctgcgt cgtcgagcgg cggggccgaa acccgtacga gcagtcccgg
     2701 aacccgacct cgaagatcgg ctccagaacc agcttcgctg ccgcctgcac gacccggtct
     2761 cgtaccgtcg ggatcccgag tggccgctgc ttgccatcgg ccttcggtat gtaccaccgc
     2821 aggaccgcct gcggccggta cgcgttctcg cgcagttcgg cctggatcgc ttcgaggaag
     2881 cgctccactc cgtcctgctc gatcgccgcg atcgtctggg cgtcgacgcc cgcggccccc
     2941 tggttccgtc tcacctggtt ccacgcctcg tacaggacgt ccctccggta gatgcggtcg
     3001 tacagcgcgt gaaactttcg acccggttgt tgcttggcca catcccgcag ccgaccttgg
     3061 aggcgtcgta cgttgtcctc gggacttcgc cctcgggg
//
LOCUS       DASVOR010001886         7401 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_165604,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001886 DASVOR010000000
VERSION     DASVOR010001886.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7401)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7401)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7401
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165604"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VF516_34965"
     CDS             <1..462
                     /locus_tag="VF516_34965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113002.1"
                     /translation="TRGDEQLLGNLAETYMMLNRLDDAIASYHRAIAAGAARVSTVYG
                     LAVALDRDGSGDQAIRQIQGQGAEGLDAFTKECIRHAVFFVPSGESAYYFALANEAAG
                     NYSAALSLWTRYIASGAHPEFQDRAREHIEALHEKHVHPESPPPELIPPPC"
     gene            456..1736
                     /locus_tag="VF516_34970"
     CDS             456..1736
                     /locus_tag="VF516_34970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113003.1"
                     /translation="MLKPSTIVAAAGLATGLALWLAAPAAAQSRRYPPEPVDKDAEKA
                     ARSNLWSAAITPERHPYQNLVRAATEALAQRTADQTGEAIKKLDAAIKLLPREPEAYR
                     LRGDAYLERTDWARCAADLAAADANRRRDDDNPRALAELHRKLGLCQARAGKLTDAER
                     TLAETAASGHAAGEVWMRLGEVRIAMGKLDEAIAALGSALETTDGNAQAMIHFLLAEA
                     YDRARRPGDALAEAADGVKLDHQLAVLHNPVIPLLGAGEADYMLGLAAHSADPARPEY
                     ALAYFRRFVQEAPDSPWRKRAEDHLRDLRGVDLPDTVTRGGSAQLDLDAVRAAARRAM
                     PQLRACLARFPTVVVELEISRSGPRTPPTDRMRPRFFTPPDGVTVRVAVGELVDRDLD
                     AVDRCLEAQANRLALPPVHEHDVFYKVMFDVVGP"
     gene            complement(1813..3777)
                     /locus_tag="VF516_34975"
     CDS             complement(1813..3777)
                     /locus_tag="VF516_34975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113004.1"
                     /translation="MADREHKSTLARGHDGRVSSDGGCRRIFGLALAVSVLAALGAAG
                     CRPPEVLIAQGASASMTSPTTLASAVTLVNQSDEAVRDILVTRISLTGGTLAVPALPL
                     HLGPIAAGESVPLHATFIGRFTAEGRHRFTVEGFFEVGHDRKRFTFDTVVVIPPRSPG
                     KGTLQSVAVHAGRVFGAPFPARRPGFDREVNTLRWTVPTGLPVAGTPRPKGTEVTRAP
                     MIPPRAPEPDDPPAIVFEANHSMGLTSAAAGTVSTIAEPSGASNGRDVVFSTANWVAA
                     YSTDGGGSFTQLDPTKIFPPDAVGYCCDQVVQYVRSIDRFVWILQGSNGNGYRLASAS
                     PRDIVTSSGTAWTYWNLTPQLFGQPGGTALDYPDLSVGDHFLYMSWDAGAPGCPPGCR
                     SGFQVVRASLDEILAGGTLTLEFTDPPDAPMAWGSHLTQDPGNEIFWAGHNTTSSMRV
                     FSLAEGSGTYFWRDVTISSWPNNALSSITPDGFNWVAGSSGFPGNAILGSTRVGRQLW
                     FAWTAGTDQRFQQPHVELAVLDRNNGFNVLQRQQIWNNSYGFAYPALATNACTGEVGL
                     SLEYGGPSDFENHVVGFWGDFVLYVTTDSNVGTSRFGDYVTIRQRPATRHHPGNLFDA
                     FGYGLDAPSPPATGTQADVHHVVFGRPSCR"
     gene            complement(3948..5939)
                     /locus_tag="VF516_34980"
     CDS             complement(3948..5939)
                     /locus_tag="VF516_34980"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8113005.1"
                     /translation="MTNRALVLCLLAACGSSAPAPATPVEPPATPPPPDRGVIVVVWD
                     GLRPDGVTAADTPNLAKLRDAGVEFTDNHATYPTFTMMNGASFATGQFSDATGYYGNV
                     LWQPGATGNDSAGKPVDFRQPVFSEDYGVLDGLKGSSKELLADTLFGAAQAAGMVTLA
                     VGKSGATYLMDTGRGGLILDERAALPLAFARELQAAGVALPPTAPNAFGAGELVLGSD
                     NGNPVEFKPALRLKDGVSSDPGDDHGSPFKGALDYLINVYADHVLPDKHPRLTVVWLR
                     DPDSTEHLFGIGTANWHDALRANDALLGKLITKLDALGLRDKTDLVVVSDHGHSNVSG
                     SQDLFPLRAVRDGAIAEIDPHGHSVSGLVRLADLLRRAGFTAFDGLGCTFLPVATGIR
                     KDGTPVYPTKVDGDGKLCGKEGQKYQAPPYKVPAELPAHAVVVAVNGGSDYIYVPDHD
                     PALVKKTVGFLQSRAEVGAIFSDDRYGAIPGTLPLGAIHARNAAGKNPDIIFSYEYDK
                     DAVVDGMPGTEMSGMLNGANFRGMHGSFSPLDVHNMLIAAGPDFRAGFKDPLPTGNVD
                     VAPTIARILGLSLPHAQGRPLLEALAHGAAVDDYRVAPDVQRPQAPATGLTVHLPTDP
                     DGKDIAPKLTTYSFELHTKALTYGKQRYTYFDFAKASRR"
     gene            complement(6004..6687)
                     /locus_tag="VF516_34985"
     CDS             complement(6004..6687)
                     /locus_tag="VF516_34985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113006.1"
                     /translation="MTRNLFVSSRLVVVVLAVATACTSEQPSAGTSAQVQQDTVLDHL
                     PRQVTAMLAFQDQMRKLWEDHIQWTRHFIISTAHDLPDAGPVTQRLLANQVDIGNAFR
                     GFYGDAVANQLTQLLTDHILIAARIVGAAKAGDNNTVTTATADWNANADQIAGLWAQI
                     NPQSWPLDVMKHHMHEHLQLTLDEAVLRLQGNFAGDIAKYEEVHAAILDMADMLSLGI
                     IRQFPNKFP"
     gene            complement(6872..>7401)
                     /locus_tag="VF516_34990"
     CDS             complement(6872..>7401)
                     /locus_tag="VF516_34990"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX8113007.1"
                     /translation="RLGRAEVVSTLPDDRVEVRVGAMRAMVPIKDVLIDTHRQARRTE
                     RGERGDRPERGERSEHGERAANGETAPGSSNGAIQLVDGVPTGGRVNARTFDTTLDVR
                     GNRVDEAVAQVDRFVDESLLAGRDAIFVVHGHGTGALRSAIRSHLASHKAIEKFRPGE
                     QSEGGDGVTVAFLRG"
BASE COUNT         1045 a         2593 c         2656 g         1107 t
ORIGIN      
        1 acgcgcggcg acgagcagct gctcggcaac ctggccgaga cctacatgat gctgaaccgg
       61 ctcgacgacg cgatcgcgag ctaccaccgc gcgatcgcgg cgggcgccgc gcgggtctcg
      121 acggtgtacg gcctcgcggt cgcgctcgat cgcgacggct cgggcgacca ggcgatccgc
      181 cagatccagg gccagggcgc cgaggggctc gacgcgttca ccaaggagtg catccggcat
      241 gccgtgttct tcgtgccgag cggcgagtcg gcgtactact tcgcgctggc caacgaggcg
      301 gccggtaact acagcgccgc gctgtcgctg tggacccgct acatcgcgag cggcgcgcac
      361 cccgagttcc aggaccgcgc gcgcgagcac atcgaggcgc tgcacgagaa gcacgtccac
      421 cccgagtcgc ctccgcccga gctcatcccg ccgccttgct gaagccgtcc accatcgtcg
      481 ccgcggccgg tctcgcgacc ggtctcgcgc tctggctcgc cgcgcccgcc gcggcgcagt
      541 cccggcgcta cccgcccgag ccggtcgaca aggacgccga gaaggcggcc cggtccaacc
      601 tgtggagcgc ggcgatcaca cctgagcgcc acccgtacca gaacctggtg cgcgccgcga
      661 ccgaggcgct cgcccagcgc accgccgatc agaccgggga ggcgatcaag aagctcgacg
      721 cggcgatcaa gctgctgccg cgcgagcccg aggcctaccg gctacgcggc gacgcctacc
      781 tcgagcgcac ggactgggcg aggtgcgccg cggacctcgc cgccgccgac gccaaccgcc
      841 ggcgcgacga cgacaacccg cgggcgctgg ccgagctcca ccgcaagctc gggctgtgcc
      901 aggcgcgcgc cggcaagctc accgacgccg agcgcacgct cgccgagacc gcggcgtcgg
      961 gccacgccgc cggcgaggtc tggatgcggc tcggcgaggt ccggatcgcg atgggcaagc
     1021 tcgacgaggc gatcgccgcg ctcggctcgg cgctcgagac caccgacggc aacgcccagg
     1081 cgatgatcca cttcctgctc gccgaggcct acgatcgcgc gcggcggccc ggcgacgcgc
     1141 tggccgaggc cgccgacggc gtcaagctcg atcaccagct cgccgtgctg cacaacccgg
     1201 tgatcccgct gctcggcgcc ggcgaggccg attacatgct cggcctggcg gcgcacagcg
     1261 ccgatccggc gcggccggag tacgcgctcg cctacttccg ccggttcgtc caggaggcgc
     1321 cggacagccc gtggcgcaag cgcgccgagg accacctccg cgacctgcgg ggcgtcgatc
     1381 tgcccgacac cgtcacgcgc ggcgggtccg cgcagctcga cctcgacgcc gtgcgcgccg
     1441 cggcccggcg cgcgatgccg cagctgcgcg cctgcctcgc ccggttcccg accgtcgtgg
     1501 tcgagctcga gatctcgcga tccgggccgc gcaccccacc gaccgaccgg atgcgaccgc
     1561 ggttcttcac cccgcccgac ggcgtcaccg tgcgcgtcgc ggtcggcgag ctcgtcgatc
     1621 gcgacctcga tgcggtcgac cgctgcctcg aggcgcaggc caaccggctc gcgctgcccc
     1681 cggtccacga gcacgacgtg ttctacaagg tgatgttcga cgtcgtcggg ccgtgaccgc
     1741 tgcgccagcg cgatccggcg ctgcagctcg ccgccgcgac gaacccgggt acgcggccgg
     1801 cgaatgctcg attcaccggc acgacgggcg gccgaacacg acgtggtgga cgtcggcctg
     1861 cgtgccggtg gccggcggcg acggcgcgtc gagcccgtaa ccgaaggcgt cgaacaggtt
     1921 gcccgggtgg tgccgcgtgg ccggccgctg gcggatcgtc acgtagtcgc cgaaccggct
     1981 cgtgccgaca ttgctgtcgg tcgtgacgta caggacgaaa tcgccccaga acccgaccac
     2041 gtggttctcg aagtcactcg ggccgccgta ctccagcgac agcccgacct cgccggtgca
     2101 ggcgttggtg gccagggccg ggtaggcgaa gccgtagctg ttgttccaga tctgctggcg
     2161 ctgcagcacg ttgaagccgt tgttgcgatc gaggaccgcg agctcgacgt ggggctgctg
     2221 gaaccgctgg tcggtccccg cggtccacgc gaaccacagc tggcgtccga cccgggtgct
     2281 tccgaggatg gcgttgcccg ggaagccgga gctgccggcc acccagttga agccgtccgg
     2341 tgtgatcgag gacagggcgt tgttcggcca gctcgagatc gtgacgtcgc gccagaagta
     2401 ggtccccgag ccctcggcga gcgagaacac ccgcatgctg ctggtggtgt tgtggccggc
     2461 ccagaagatc tcgttgccgg ggtcctgcgt gaggtggctg ccccacgcca tcggcgcatc
     2521 cggcggatcg gtgaactcga gggtgagcgt gccccccgcc aggatctcgt ccagcgacgc
     2581 ccgcacgacc tggaagcccg agcgacaacc cggcgggcaa ccgggcgcgc cggcgtccca
     2641 gctcatgtac aggaagtggt cgccgaccga caggtcgggg taatcgaggg cggttccgcc
     2701 gggctggccg aacagctgcg gcgtcaggtt ccagtaggtc caggccgtgc cgctgctggt
     2761 gacgatgtcc cgcgggctgg ccgacgcgag gcgatagcca ttgccgttgc tgccctggag
     2821 gatccagacg aagcggtcga tgctgcgcac gtactgcacg acctggtcgc agcagtaacc
     2881 gacggcgtcg ggcggaaaga tcttcgtggg gtcgagctgg gtgaacgagc cgcccccatc
     2941 ggtggaatac gccgctaccc agttcgccgt cgagaacacc acgtcgcggc cgttgctggc
     3001 gccgctgggc tccgcgatgg tgctgaccgt gccggcggcc gccgacgtga ggcccatcga
     3061 gtggttggcc tcgaacacga tcgcaggcgg gtcgtcgggc tccggtgcac gcggcgggat
     3121 catcggcgct cgcgtgacct cggtgccctt cggcctcggc gttccggcga ccggcaaccc
     3181 ggtgggcacc gtccagcgca gcgtgttcac ttccctgtcg aagccgggcc gtctcgccgg
     3241 gaacggcgct ccgaacaccc tgccggcatg gacggcgacg ctctggagcg ttcccttccc
     3301 gggactgcgc ggtgggatga cgacgacggt gtcgaacgtg aagcgcttcc ggtcgtggcc
     3361 gacctcgaag aagccttcga cggtgaatcg atggcggccc tcggcggtga accggccgat
     3421 gaacgttgcg tgcagcggca ccgactcgcc ggctgcgatc ggtcccaggt ggagcggcag
     3481 cgcgggcacg gcgagggttc caccggtcag cgagatcctc gtcaccagga tgtccctcac
     3541 cgcctcgtcg ctctggttga cgagggtcac agccgacgcc agggtcgtgg gactcgtcat
     3601 gctggcgctc gcgccctgcg cgatcaggac ctccggaggt cggcatcccg ccgcgcccag
     3661 cgccgcgagc accgagaccg cgagcgccag cccgaagatc cgccggcaac caccatcgct
     3721 gctgacccgg ccatcatggc cgcgcgcgag cgtactcttg tgttccctgt ccgccattgc
     3781 cccgtcctcc tcgtctacac atcgaatcga ttcctcgact catgaggagg ataatcgaca
     3841 ctgccggacg ggcaagcgaa tgacagcgat ccgcgagtcc gcgcatcggc gcacggggac
     3901 tgcctccaca catccgagca ccgcggtgcc cgcgcgctcg cgcgcgatca gcgccgcgag
     3961 gccttggcga agtcgaagta cgtgtagcgc tgcttgccgt acgtgagcgc cttggtgtgg
     4021 agctcgaagc tgtacgtcgt cagcttcggc gcgatgtcct tgccgtcggg gtcggtcggc
     4081 aggtggacgg tgaggccggt cgccggtgcc tgcgggcgct ggacgtcggg cgcgacgcga
     4141 tagtcatcga ccgcggcacc gtgggcgagc gcctcgagca gcggccggcc ctgggcgtgc
     4201 ggcaggctga gcccgaggat ccgggcgatc gtcggcgcga cgtcgacgtt gccggtcggc
     4261 agcgggtcct tgaagccggc gcggaagtcg gggcccgcgg cgatcagcat gttgtggacg
     4321 tcgagcgggc tgaagctgcc gtgcatgcct cggaagttgg cgccgttcag catgccgctc
     4381 atctcggtgc ccggcatgcc gtcgacgacc gcgtccttgt cgtactcgta gctgaagatg
     4441 atgtcggggt tcttgccggc ggcgttgcgg gcgtggatgg cacccagcgg cagcgtgccg
     4501 gggatggcgc cgtagcgatc gtcgctgaag atcgcgccga cctcggcgcg cgactgcagg
     4561 aagccgacgg tcttcttgac cagcgccgga tcgtgatcgg gcacgtagat gtagtcgctg
     4621 ccgccgttga ccgcgacgac caccgcatgg gccggcagct cggccgggac cttgtagggc
     4681 ggcgcctggt acttctggcc ctccttgccg cacagcttgc catctccgtc gaccttcgtc
     4741 gggtagaccg gcgtgccgtc cttgcggatg cccgtcgcga ccgggaggaa cgtgcagccg
     4801 aggccatcga acgccgtgaa gccggcccgg cgcagcaggt cggcgagccg aaccagtcca
     4861 gataccgaat ggccgtgcgg gtcgatctcg gcgatggctc cgtcccgcac cgcacgcagc
     4921 gggaacagat cctgcgaccc cgagacgttg ctgtggccgt ggtcggacac cacgacgagg
     4981 tcggtcttgt cgcgcagccc gagcgcgtcg agcttggtga tcagcttgcc gagcagcgcg
     5041 tcgttggcgc gcagcgcgtc gtgccagttg gcggtgccga tgccgaacag gtgctcggtg
     5101 gagtccggat cgcgcagcca caccaccgtg agccgcgggt gcttgtccgg cagtacgtgg
     5161 tccgcgtata cgttgatcag gtagtcgagc gcgcccttga acggactgcc gtggtcatcg
     5221 ccggggtcgc tggacacacc gtccttgagc ctgagggccg gcttgaactc gaccgggttg
     5281 ccgttgtcgg acccgagcac cagctcgccg gcgccaaacg cattcggcgc ggtcggcggc
     5341 agcgcgacgc ccgccgcctg cagctcccgg gcaaacgcca gcggcagggc ggccctctcg
     5401 tcgaggatca gtccgccgcg cccggtgtcc atcaggtagg tggcgccgct cttgccgacc
     5461 gccagcgtga ccatgccggc ggcctgggcg gcgccgaaca gcgtgtcggc caggagctcc
     5521 ttgctcgaac ccttcagccc gtcgagcacg ccatagtcct ccgagaacac cggctggcga
     5581 aagtcgaccg gcttgcccgc cgagtcgttg ccggtcgcgc cgggctgcca cagcacgttg
     5641 ccgtagtagc cggtcgcgtc ggagaactgg ccggtcgcga agctcgcgcc gttcatcatc
     5701 gtgaacgtgg gatacgtcgc gtggttgtcg gtgaactcga cgccggcgtc gcgcagcttc
     5761 gccaggttcg gcgtgtcggc ggcggtcacg ccgtccggcc ggaggccgtc ccagacgacg
     5821 acgatcacgc cccggtcggg gggaggtggg gtggccggcg gctcgaccgg cgtggcgggc
     5881 gccggcgcgc tcgatccgca cgcggcgagg agacacagga ccagggcccg gttcgtcatg
     5941 ccgggcagtg aaccacggac cgctggcgcg cgggtgacaa cgtagcggca gatcgggctc
     6001 gccctacggg aacttgttgg ggaactgccg gatgatcccg aggctgagca tgtctgccat
     6061 gtcgaggatc gcggcgtgga cctcctcgta cttggcgatg tcgccggcga agttgccctg
     6121 caggcgcagc acggcctcgt cgagggtgag ctggaggtgc tcgtgcatgt ggtgcttcat
     6181 gacgtcgagc ggccacgact gcgggttgat ctgcgcccac aggccggcga tctggtcggc
     6241 gttggcgttc cagtccgcgg tcgccgtcgt gacggtgttg ttgtcgcccg ccttggcggc
     6301 gcccacgatc ctggcggcga tcaggatgtg atcggtcagg agctgggtga gctggttggc
     6361 gaccgcatcg ccgtagaacc cgcgaaacgc gttgccgatg tcgacctggt tggccagcag
     6421 ccgctgcgtg accggtccgg cgtcgggcag atcgtgcgcg gtgctgatga tgaagtgccg
     6481 cgtccactgg atatgatcct cccagagctt ccgcatctgg tcctggaacg cgagcatcgc
     6541 ggtcacctgc ctgggcaggt ggtcgaggac cgtgtcctgc tgcacttgcg ccgaggtgcc
     6601 cgcagacggc tgttcacttg tacaggccgt cgcgaccgcc agcaccacca ccacgagacg
     6661 tgacgagacg aacaaattcc tggtcatcgc atgctccttg ggtgcgggtt cgcctggtga
     6721 ggcgagagtc ctccccattg gagtggccgc gcgcgccagg cgttacacgc tcgcaatccc
     6781 tcccccatcg ctcgttacgg tctggaatgc cgctctgggg atcaggcgac agcaggatcg
     6841 taagccaggt cgcagcgacg atggccccgc gctagccgcg caggaacgcg acggtgacgc
     6901 cgtcgccgcc ctcgctctgc tcgccgggcc ggaacttctc gatcgccttg tgcgacgcca
     6961 ggtggctgcg gatcgccgag cgcagcgcgc cggtgccgtg gccgtggacg acgaagatcg
     7021 cgtcgcggcc ggcgagcagg ctctcgtcga cgaagcggtc gacctgggcg acggcctcgt
     7081 cgacccggtt gccgcggacg tcgagcgtgg tgtcgaacgt gcgcgcgttg acccggcccc
     7141 cggtgggcac gccgtcgacg agctggatcg cgccgttgga cgacccgggc gcggtctcgc
     7201 cgttcgccgc gcgctcgccg tgctcgcttc gctcgccccg ctccgggcga tcgccgcgct
     7261 cgccccgctc ggtgcggcgg gcctggcggt gggtatcgat cagcacgtcc ttgatcggca
     7321 ccatcgcgcg catcgcgccg acccggacct cgacccgatc gtcgggcagg gtgctgacca
     7381 cctcggcgcg gccgagccgg g
//
LOCUS       DASVOR010001887         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_20916,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001887 DASVOR010000000
VERSION     DASVOR010001887.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20916"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1510)
                     /locus_tag="VF516_34995"
     CDS             complement(<1..1510)
                     /locus_tag="VF516_34995"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8113008.1"
                     /translation="MRRFWIGLILVLAVSFGVLGFVGSRIYQLAPPIPDRVVGRDGTV
                     VFEPGQIAAGQDVWRSFGGMELGSIWGHGAYVAPDWTADWLHRELVGVLDGWAAAAGG
                     PAYARLPPEQQAALRERLAHEYRANTYDAAARTITVSPERARAIEANIAYYTKLFAEG
                     RDAYALPPGTVVEPARARALAAYFFWSAWAAATDRPGESITYTSNWPHEPLIGNRPTP
                     DAVVWTGVSILMLLAGIGGLGFWHAARRKDDGPIALPAADPMLGASPTPSQLATVKYF
                     WVVCGLFVLQLLVGIITAHYGVEGSSFYGIPLAKILPYSVVRTWHVQLGIFWIATAWL
                     AAGLAIAPSVGKEPRRQKLLVNVLFGALVVVVLGSLVGELFAVHGVLGADGVWWFGHT
                     GYEYVDLGRFFQIALLVGLVLWVVLVGRALWPALRRRDAQRPLLLMLLISTIAIGGFY
                     FAALGAGRHTNLAIAEYWRWWVVHLWVEGFFEVFATVIIAFLFTRLGLIKAAT"
BASE COUNT          293 a          634 c          648 g          200 t
ORIGIN      
        1 cagtggccgc cttgatcagg ccgagccggg tgaacaggaa cgcgatgatc accgtcgcga
       61 acacctcgaa gaagccctcg acccacaggt ggacgaccca ccagcgccag tactcggcga
      121 tcgccaggtt ggtgtggcgg cccgcgccga gcgcggcgaa gtagaacccg ccgatcgcga
      181 tcgtcgagat cagcagcatc agcagcagcg ggcgctgcgc gtcgcggcgg cgcagcgccg
      241 gccacagcgc ccggccgacc agcacgaccc acaggaccag gccgacgagc agcgcgatct
      301 ggaagaaccg gccgaggtcg acgtactcgt agccggtgtg gccgaaccac cacacgccgt
      361 cggcgccgag cacgccgtgc acggcgaata gctcgccgac cagcgagccg agcacgacga
      421 cgacgagcgc gccgaacagc acgttgacca gcagcttctg gcggcgcggc tccttgccga
      481 ccgacggcgc gatcgccaga ccggcggcga gccacgcggt cgcgatccag aagatgccga
      541 gctggacatg ccaggtccgg acgaccgagt acggcaggat cttcgcgagc gggatgccgt
      601 agaacgacga gccctcgacg ccgtagtgcg cggtgatgat cccgaccagg agctggagca
      661 cgaacaggcc gcacacgacc cagaagtact tcacggtggc gagctgcgac ggtgtcggcg
      721 acgcgccgag catcggatcg gcggccggca gcgcgatcgg gccgtcgtcc ttgcggcgcg
      781 cggcgtgcca gaacccgagc ccgccgatgc cggcgagcaa catcaggatg ctcacgccgg
      841 tccacacgac cgcgtcgggg gtcggccggt tgccgatcag cggctcgtgc ggccagttgc
      901 tggtgtaggt gatcgattca ccggggcgat cggtcgcggc ggcccacgcc gaccagaaga
      961 agtacgccgc cagcgcccgg gcgcgggccg gctcgaccac ggtgcccggg ggcagcgcgt
     1021 aggcgtcgcg gccctcggcg aacagcttcg tgtaataggc gatgttggcc tcgatcgccc
     1081 gcgcccgctc gggcgacacg gtgatcgtcc gcgcggcggc gtcgtacgtg ttggcccggt
     1141 actcgtgggc cagacgctcg cgcagcgcgg cctgctgctc gggcggcagc cgtgcgtacg
     1201 ccggcccgcc ggccgcggcc gcccagccat cgagcacgcc gaccagctcg cggtgcagcc
     1261 agtccgccgt ccagtccggc gcgacgtagg cgccgtggcc ccagatcgat ccgagctcca
     1321 tgccaccgaa cgagcgccag acgtcctggc ccgcggcgat ctgaccgggc tcgaacacca
     1381 ccgtcccgtc gcggccgacc acgcgatcgg gaatcggcgg agcgagctgg tagatccgcg
     1441 agccgacgaa cccgaggacc ccgaacgaca cggcgagcac caggatgaga ccgatccaga
     1501 atcgacgcat gagccgctga cgagtgcacg gcgtgcgcca gcgccagagc gcgttcttgc
     1561 gcccggcgcc ggggagacgc gcagcgattg cgcggggcac gcggcggagc cgcagcggtt
     1621 gcgacctgcg cgtgatttgc gctgcggggt tccggcgatc accggcggcg cgggcggccg
     1681 ggccgcggcg acggctcggg gtctgcatcg ggcgccaggc cgccgacccg ctcggagtcg
     1741 gtcagcttgc gaccgtcgtc ggtgaactcc cggtc
//
LOCUS       DASVOR010001888         3653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_49413,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001888 DASVOR010000000
VERSION     DASVOR010001888.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49413"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VF516_35000"
     CDS             <1..356
                     /locus_tag="VF516_35000"
                     /inference="COORDINATES: protein motif:HMM:NF040299.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6011 domain-containing protein"
                     /protein_id="HEX8113009.1"
                     /translation="SFTVLNDMIAAIGEGYFGLERREASSAGNVTTFFRIKRTYKGGP
                     VRIMQVLGGPGGWRYEPLGVTAQFHALRHIGADVEKAGLCFAEKTSCCRRCHSPLTNT
                     VSSSRGYGPDCIKLI"
     gene            412..702
                     /locus_tag="VF516_35005"
     CDS             412..702
                     /locus_tag="VF516_35005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113010.1"
                     /translation="MDPVETLRELRELAAEAIEDGQRGGTPTFARLRRTAEIAAALAE
                     KFQELDDWMRGQRRGYSPWNTERERADSAGMPQGVARTGRRWVAPDETNKES"
     gene            704..1318
                     /locus_tag="VF516_35010"
     CDS             704..1318
                     /locus_tag="VF516_35010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113011.1"
                     /translation="MKIKDLAAAIAAAGGPVDVYWYGRNGKYDFDRQRENPEECRWGT
                     DGRLYGRYYASGNYRPVEPGTKLRATHVRLRRVEREWPADTPRHVRTAYDTDMYAPLA
                     QVVGLYDEVAGQRREMVAATRAALDATRRAEQVREMRLNQVLDRAAVLGHPLGDVRLS
                     REGVTVGGVSFDVLEALVNDLVVKTAALDALDGRGLVVEDTDPA"
     gene            1315..1704
                     /locus_tag="VF516_35015"
     CDS             1315..1704
                     /locus_tag="VF516_35015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113012.1"
                     /translation="MTGQVRRGRGLLLPPRLVVDRYVNQQESLRAIARDYQVAPTTVR
                     RLLLDEGVTLRDHDPRTGGHLDNRGRPPTPLPAAEMAERYAAGDGLRDLSRRYQIAPS
                     TAGRAVVRGGGTLRAPGRPREGRGGHA"
     gene            1691..1924
                     /locus_tag="VF516_35020"
     CDS             1691..1924
                     /locus_tag="VF516_35020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113013.1"
                     /translation="MDTRSHIQCSEAFSHGPADAYGRCPWCRQKYTHAVHPRGTASIA
                     KLAEAYNTTYDPDHGALGVTELERRHRAGLDTW"
     gene            1940..2347
                     /locus_tag="VF516_35025"
     CDS             1940..2347
                     /locus_tag="VF516_35025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113014.1"
                     /translation="MDLGDYRDDDRATWLGASDPLDERYLIDRAEHEAHAEGNEEEPD
                     DPGEIEAPGNLTTSIPVPTLVELVDHLANMAYLWGSIAAMYGDNDPTARSYKTTSEEI
                     RAEIQRRLDLVAERTGDDDGTTARIVEILNGRP"
     gene            2344..2637
                     /locus_tag="VF516_35030"
     CDS             2344..2637
                     /locus_tag="VF516_35030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113015.1"
                     /translation="MSDDWCEVMEMYRRSCAHCRPPAERRRLDALAVELDLKGGNTAP
                     RRDVGGVSPAFYGPLIAEHSGKCEAECGDAIATGDEITRYSKGWAHHRCVYLG"
     gene            2676..2763
                     /locus_tag="VF516_35035"
     tRNA            2676..2763
                     /locus_tag="VF516_35035"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
     gene            2953..>3653
                     /locus_tag="VF516_35040"
     CDS             2953..>3653
                     /locus_tag="VF516_35040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113016.1"
                     /translation="MKRWIIRIAVVAVAVLGTAFAGAVGAHWGAASVSVPLVSPCDQG
                     WYVNDDEDGAPASEGPDRRPEATTGGLRFESADLVHHATSLPLASLTPGSYVAAPAPD
                     QPSFFSVEVRDTTGAYGTLRWNPTTHLWSIVIGAGTGPGGPATDGTFTGADPSALLSG
                     KISKWGAFTTSTTVVSFGVGYTQNPPGTVATVVSSVTFGGKTYPLTCPSPSASASASA
                     SANTSASASASASASA"
BASE COUNT          578 a         1327 c         1295 g          453 t
ORIGIN      
        1 tgtcgttcac ggtgctcaac gacatgatcg ccgccatcgg cgagggctac ttcggccttg
       61 agcggcgcga ggcgagcagc gcgggcaacg tcaccacgtt cttccggatc aagcgcacgt
      121 acaagggcgg cccggtgcgc atcatgcagg tgctgggcgg cccgggtggc tggcggtatg
      181 agccgctcgg cgtgaccgcg cagttccacg cgctgcgcca catcggcgcc gacgtcgaga
      241 aggcggggct gtgcttcgcc gagaagacct cgtgctgccg gcgctgccac tcgcccctga
      301 ccaacaccgt gtcgtccagc cggggctacg gccccgactg catcaagctc atctgaccac
      361 tcaggcggcc ccgggcaacc ggggccgccc ctcccccgaa aggatcaacc gatggacccc
      421 gtggaaaccc tgcgcgagct gcgcgaactc gccgccgagg cgatcgagga cggccagcgc
      481 ggcggtaccc ccaccttcgc tcgattgcgc cggacggccg agatcgccgc cgccctcgcc
      541 gagaagttcc aggagctgga cgactggatg cgcggccagc ggcgcggcta cagcccgtgg
      601 aacaccgagc gggagcgggc cgacagcgcc ggcatgcccc agggcgtggc acgcaccggc
      661 cgccggtggg tggcccccga cgagaccaac aaggagagct gacatgaaga tcaaggacct
      721 ggcggccgcg atcgcggcgg ccggcggccc ggtcgacgtc tattggtacg gccgcaacgg
      781 caagtacgac ttcgaccgcc agcgcgagaa ccccgaggag tgccgctggg gcaccgacgg
      841 ccggctgtac ggccggtact acgccagcgg caactaccgc ccggtggagc ccggcaccaa
      901 gctgcgggcg acccatgtgc ggctgcggcg cgtcgagcgc gagtggccgg ccgacacgcc
      961 caggcacgtg cgcaccgcct acgacaccga catgtacgcg ccgttggccc aggtggtcgg
     1021 cctgtacgac gaggtggccg gacagcggcg cgagatggtc gccgccaccc gggcggccct
     1081 cgacgcgacc cgccgtgccg agcaggttcg tgagatgcgg ctcaaccagg tgctggatcg
     1141 cgcagcggtg ctcggtcacc cgctcggcga cgtgcggctg tcccgggaag gcgtgaccgt
     1201 cggtggcgtc tctttcgacg tcctggaggc cctcgtcaac gacctggtgg tcaagacggc
     1261 agcgctcgac gcgctggacg gtcgcggcct cgtcgtcgag gacacggacc cggcatgacc
     1321 ggccaggtgc gccggggtcg ggggctgctg ctcccgcccc ggctcgtcgt cgaccggtac
     1381 gtcaaccaac aggagtcgtt gcgcgccatc gcccgcgact accaggtggc gcccaccacg
     1441 gtgcgccgcc tgctgctcga cgagggcgtc acgctgcgcg accacgaccc gcgcaccggc
     1501 gggcacctcg acaaccgggg ccggccaccc acccccctgc cggcggcgga gatggcggaa
     1561 cggtacgcgg ccggtgacgg cctgcgcgac ctgtcgaggc gctatcagat agcaccctcg
     1621 acggccggcc gggccgtcgt gcgcggcggc ggcacgctgc gggcgccggg ccgaccccga
     1681 gagggtcgcg gtggacacgc gtagccacat ccagtgctcc gaggcgttca gtcacgggcc
     1741 ggccgacgcg tacggccgct gcccgtggtg ccgccagaag tacacccacg ccgtgcatcc
     1801 gcgcggcacg gcctcgatcg cgaagttggc cgaggcctac aacaccacgt acgaccccga
     1861 ccatggcgcc ctcggcgtca ccgaactgga gcgccggcac cgggccggcc tcgacacctg
     1921 gtaaggaggt cttcccccaa tggaccttgg agactaccgc gacgacgacc gggccacctg
     1981 gctcggtgcg agcgacccgc tcgacgagcg gtacctgatc gaccgggccg agcacgaggc
     2041 gcacgccgag ggcaacgaag aggagcccga cgaccccggc gagatcgagg cgcccggcaa
     2101 cctcacgacg tcgattccgg ttccgacact cgtcgagctg gtcgaccacc tcgccaacat
     2161 ggcctacctg tggggctcga tcgccgccat gtacggcgac aacgacccga ccgcccgcag
     2221 ctacaagacg accagcgagg agatccgggc cgagatccag cgccggctcg acctcgtcgc
     2281 cgagcgcacc ggcgacgacg acggcaccac ggcgcggatc gtggagatcc tgaacggccg
     2341 gccatgagcg acgactggtg cgaggtcatg gagatgtacc gccgctcgtg cgcgcactgc
     2401 cggccaccgg cggagcggcg ccggctcgac gccctcgccg tcgagctgga cctgaagggc
     2461 ggtaacaccg caccacggcg tgatgtcggc ggagtcagtc cggccttcta tgggccgctg
     2521 atcgccgaac actccggcaa gtgcgaggcc gaatgcggcg acgcgatcgc caccggcgac
     2581 gagatcaccc ggtacagcaa ggggtgggcg catcatcgat gcgtctacct cggttgacat
     2641 acggcgtgtg tcaggatgca cactggcaca cgttcggggc ggtagctcag ttggcagagc
     2701 agccgtccga cccgctcgca cgggccggac ggtgggtcgc aggttcgaat cctgcccgcc
     2761 cctcgaccgc gcaccccgcg cggtagggag gaaggaggcc cgcatgttca tggcaggtcg
     2821 aggcaagggc tacgccgtgg cccgcaagcc caagagtgcg atggacctgc gcgccggtgc
     2881 cggcggagtg agccggcacc tgtcggcgaa agacccgggc cgctcccgta aggcgcgtcg
     2941 gaaaggacgc tcatgaaaag gtggatcatc cgtatcgccg tggtcgcggt cgcggtgctc
     3001 ggtaccgcgt tcgccggtgc ggtcggtgcc cactggggtg cggcgagcgt gtcggtgccg
     3061 ctcgtgtcgc cgtgcgatca gggctggtac gtcaacgacg acgaggacgg cgcgccggcc
     3121 agcgagggac cggaccgccg gcccgaggcg accaccggcg ggctgcgctt cgagtcggcg
     3181 gacctcgtcc accacgccac cagcctgcca ctggccagcc tcacgcccgg ctcgtacgtc
     3241 gcggcgccgg ccccggatca gccgtcgttc ttcagcgtcg aggtgcgtga cacgaccggc
     3301 gcgtacggaa ccctgcggtg gaacccgacc acgcacctgt ggtcgatcgt gatcggcgcc
     3361 ggtaccggcc ccggtggccc tgcgaccgac gggacgttca cgggcgccga cccctcggcc
     3421 ctgctgtccg gcaagatctc caaatggggt gccttcacca cgagcacgac cgtggtcagc
     3481 ttcggggtcg gctacaccca gaacccgccc ggcaccgtgg cgacggtggt ctcgtccgtc
     3541 acgttcggcg gcaagaccta cccgctgacc tgccccagcc cgtcggcgag cgccagcgcg
     3601 tccgcgtcgg ccaacacgag cgccagcgcg tcggcctcgg ccagcgcgag cgc
//
LOCUS       DASVOR010001889         1898 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340771,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001889 DASVOR010000000
VERSION     DASVOR010001889.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1898
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340771"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF516_35045"
     CDS             complement(<1..256)
                     /locus_tag="VF516_35045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113017.1"
                     /translation="MHPPELDRRSERERFDHGAEGARYGLSRELSLVIWQRAWADSMD
                     AAGGPGMADALRRFHAIAARLAARGGRLQPDVGRLTRVGVE"
     gene            579..>1898
                     /locus_tag="VF516_35050"
     CDS             579..>1898
                     /locus_tag="VF516_35050"
                     /inference="COORDINATES: protein motif:HMM:NF015148.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEX8113018.1"
                     /translation="MSNVAVREATLRAVVMGVGFGAVFGAANAYLGLRVGLTIATSIP
                     VAVMTVALPRIFGARVSILEANLAQTIGSASTSLATGAIFTLPALWLWLSPPGYLQIA
                     GLALCGGVLGVSAMIPLRRLLIVRGGPELPYPEGRACAEVLRATAEGDSSGRWIFYGL
                     GIGAAIKLVLSLVALVPSNVHWALPAIPRGELAIEIAPALVGVGFILGYRQSAVLVSG
                     AVISALMIIPLVATLLAGGPAPVPEAGALWGSYVRYIGTGAVACAGILTVLRGLPAMA
                     AAFTAVIRGLGDDGGGAPDRTQRDVPGWVIGTGVVAVVAAVALIPGLLPGGLRFTERL
                     APAAGVAVFGLVFVAVASRIVGIVGVSSQPTSGVTLVTLIGMGLLFSQLGLTGDGARL
                     ALLTCGTIVATAASKAGDISQDLKTGQLVGATPALQQLGQYLGAATAC"
BASE COUNT          200 a          688 c          720 g          290 t
ORIGIN      
        1 gctcgacccc gacgcgcgtc aaccggccga cgtctggctg gagccggcca ccccgggctg
       61 ccaggcgcgc ggcgatcgca tgaaatcgcc gcagcgcgtc cgccatgccc ggcccacccg
      121 ccgcatccat cgagtccgcc cacgcccgct gccagatcac cagcgacagc tcccgagaca
      181 ggccgtaccg cgccccttcc gcgccgtggt cgaaccgctc gcgctcgctc cgccggtcga
      241 gctcgggcgg gtgcatccag cgcatcattg cggactcgtt ctgccgcagc cgcccgcagc
      301 agcgccggcg cacaggctcg ccgtgtcccg aggtcgatct gccgcgttgc gcctggcacc
      361 cgagcatgtg ggcatccctg gtagataagg ccgcgccctg ccgttcgcga tgaccgacga
      421 tgacggcgga ttacggccga taacgggcgg ccccggtagg gctttgacat cggcgcggag
      481 ccgccgcatc gagctacggc gcggcgagct tcgcgagcca ggcgcgcggg acgccgtggc
      541 ggaggcgcct ccgggcgccc gtgctacgtt cgcgagccat gtcgaacgta gcggtgcgcg
      601 aggcgaccct gcgcgcggtg gtgatgggcg tcgggttcgg cgccgtgttc ggggccgcga
      661 atgcctacct cggcctgcgc gtcgggctca ccatcgccac ctcgatcccg gtggcggtca
      721 tgaccgtcgc gttgccgcgg atcttcggcg cccgggtctc gatcctcgag gccaacctgg
      781 cgcagacgat cggctcggcg tcgacctcgc tggccaccgg ggcgatcttc acgctgccgg
      841 cgctgtggct gtggttgtcg ccgcccgggt acctgcagat cgccggcctg gcgctgtgcg
      901 gcggcgtgct cggggtctcg gcgatgatcc cgctgcggcg gctcttgatc gtgcgcggcg
      961 gtcccgagct gccgtacccc gagggccggg cgtgcgccga ggtgctgcgc gcgaccgccg
     1021 agggcgacag ctccgggcgc tggatcttct atggactcgg catcggcgcc gcgatcaagc
     1081 tcgtgctgtc gctggtcgcg ctggtgccga gcaacgtgca ctgggcgctg cccgcgatcc
     1141 cgcgcggcga gctggcgatc gagatcgcgc cggcgctggt cggcgtcggc ttcatcctcg
     1201 gctatcgcca gtcggcggtg ctcgtgtccg gcgccgtgat ctcggcgctg atgatcatcc
     1261 cgctggtcgc gacgctgctc gccggcggcc ccgccccggt gcccgaggcc ggcgcgctgt
     1321 gggggagcta cgtgcgctac atcggcaccg gcgcggtcgc ctgcgcgggc atcctcacgg
     1381 tgctgcgcgg gctaccggcg atggcggccg cgttcaccgc ggtgatccgg gggctgggcg
     1441 acgatggcgg cggggcaccg gatcgcaccc agcgcgacgt gccgggctgg gtgatcggca
     1501 ccggcgtggt ggcggtggtg gccgccgtgg cgctgatccc ggggctcttg cccggcggcc
     1561 tgcgcttcac cgagcggctg gcgccggccg ccggggtcgc ggtgttcggg ctggtgttcg
     1621 tcgcggtggc gtcgcgcatc gtcgggatcg tcggcgtgtc gtcgcagccg acgtcggggg
     1681 tcacgctggt cacgctgatc ggcatgggcc tcttgttcag ccagctcggg ctgaccggcg
     1741 acggcgccag gctggcgctg ctgacctgcg ggacgatcgt cgcgaccgcg gcgtcgaagg
     1801 ccggcgacat ctcgcaggac ctcaagaccg ggcagctggt cggcgcgacc ccggcgctcc
     1861 agcagctcgg gcagtacctc ggcgcggcga ccgcgtgc
//
LOCUS       DASVOR010001890        12916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_49415,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001890 DASVOR010000000
VERSION     DASVOR010001890.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12916
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49415"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(234..635)
                     /locus_tag="VF516_35055"
     CDS             complement(234..635)
                     /locus_tag="VF516_35055"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113019.1"
                     /translation="MADLLIVDDDPDMAELLGEALVYHGHQVRIARDGEDGLRALSVR
                     LPDAVLLDVEMPILDGPGMACQMFVRNCGLETIPIILLSGVIDLEQVAARVGTPYFLG
                     KPYALGAVTALCDRALTERTPPAPTLEGSTC"
     gene            complement(610..2070)
                     /locus_tag="VF516_35060"
     CDS             complement(610..2070)
                     /locus_tag="VF516_35060"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8113020.1"
                     /translation="MSLRRSFLVFAAILAVIGSGVAIALILLTTNLHRAAKEVGAEVE
                     SLRVGEGLELGLISLREATDPLARGATESVVRRRLTEAAGYMGSTQDKEIFSDLKRQV
                     DQYIVALDRAAAGAADPAQVRDATRAAFEPAFASARQWMQLNVEQGRAAERTAARWAE
                     LADVIGVAAILLLVVGLGGLAWWLQRRTFRPALQLAGAIDRYTRGDRTARASEQEPEE
                     FRTIARGFNDMAQTLDRQRADQLAFLAGVAHDIRNPLGALKMATDMIPPDQALPSEPR
                     VRQLVARISRQIGRLERMVYDFLDASRIESGHLDLRLEDCDLRELVRITLDLFEPATA
                     THALIVSAPDQPVRVRCDPMRVEQVLANLVNNAIKYSPPGGSVRVTVAERPETVVVSV
                     ADEGVGMSADDLEHVFDPFRRGGGAVQAAPGAGLGLFVARRIVEAHDGEITVASAKGA
                     GSTFTVRLPVDPGHGRRADGAMVPRQRRDGRSADRR"
     gene            complement(2339..3682)
                     /locus_tag="VF516_35065"
     CDS             complement(2339..3682)
                     /locus_tag="VF516_35065"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8113021.1"
                     /translation="MKAGRVSVMVVALAASARADIAIKQVGFSDGDDWEPAIAVDGRY
                     VYVAWAHIGAPQQDSSGAVCRTRGVTSYAYFQRSTSSGATWEPYILPRCPVSGTQIDL
                     QVAVGPHHRVYVSYMDGRQANTNIELIYSDDRGATWSSPINVASRGGGDKDVLLVDPG
                     GGVMVAYEHLSSSYVAYSPDPTTTAFSHIKVSPPPGAGHGTSLATGGIRDSHGNTYLV
                     WTDVTGNSKHDSYLWLARSSDHFATYTTSLIDRSYAADVDVGAGWDYWGAAVQIAAIP
                     RAGNDRLVVIYNAGTSAGAAQRIYTKYSDTLGASWNIPYSASSYPSGAELSTAPIGAW
                     HGFPAIAATAAGVKVIWQDNRVKYPCTSSVTPGACGRFNSYTRTSTDGVTWTSEARVQ
                     LPTTHRYQVTTPAPGGFYHPYGDYTSMATDGTTTWGVWGEGTSYNGPGTIYTATF"
     gene            3818..5425
                     /locus_tag="VF516_35070"
     CDS             3818..5425
                     /locus_tag="VF516_35070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HEX8113022.1"
                     /translation="MDATITSLQTLLLLVAVLVVVALVAQRLRLAPPILMVIAGIAMA
                     LVPGLPRFELSPEFVLLFVLPPVIYSAGVSMSWREFRFNLRPIGLLAIGGVVFTACAI
                     AAVLHYALGFAWELGFLLGAIVAPPDALAPISIARDLGVPRRILVVLEGEGLANDATA
                     LVLYRFAVAAISIGAFSLARAVGVFGLILAGEILYGVLIGWLSLRLRRWARNTRVEIA
                     LSLMTPYVAFWPCAHLGGSGVLAAVATGLYVSWKGPLQISSATRLQGIFFWDLTLYLI
                     NGLVFLYTGLQLRTLVERVHLGELHWMLLATALTTAVAIAARFVWTYPVAYLPRWLSK
                     QLAARDPAPAWQVPFMIAFTGVRGVVSLAAALAIPTTLRDGRPFPHRDTFLFIAFGVI
                     LITLVGQGLALPWLIHKLGLARGGEEESRLELEQQRAAREQVLDSAARVLDQIANEHT
                     LPDDVVRQVQTHIANQRRQIPSDLGDSLVLAQSVSELRAELIGEQRRVLHELLRGGKL
                     SDESRRRLERELDLEEETLRHRTDLAL"
     gene            complement(5705..7198)
                     /locus_tag="VF516_35075"
     CDS             complement(5705..7198)
                     /locus_tag="VF516_35075"
                     /inference="COORDINATES: protein motif:HMM:NF024677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAFs-binding domain-containing protein"
                     /protein_id="HEX8113023.1"
                     /translation="MAWPPEAMARRRPSDHAIVPPRREVLVSTILRPLCFVAMPFGTK
                     RDPTGRPDIDFDRIYSEAIEPAIRDAELEPLRADGERTGGIIHKAMFERLLLCDFAVA
                     DLTTANANVLYELGVRHATRPQTTLAIYAKHSPLPFDVNLMRSLSYEIGTDNRFGAPE
                     AAALRVALARRLRELREVFHDQATVDSPLFQLLEGYPSPDLARLRTDLVRDRIRASEA
                     LRARLTEARRLNARAGAQALEDIEQSLGSLDGAELGVVVDLFLSYRAVGAHDAMIQLY
                     DRMPEVLRRTVLVREQLAFALNRRAGKDPARRADRDRAIETLNELVEQHGPSSETLGI
                     LGRIYKDLWDEAQRAGDALTARGYLRKAIDTYVRGFEADWRDAYPGVNAVTLLELDGS
                     PRSLAERDRMVPVVRYAVEQRLRTTEPDYWDHATLLELAVLAGDEVASADRLADALAA
                     VRERWEPETTARNLRLLRDARAARGTAQAWLDPIIAALEARGAGGMP"
     gene            8011..8277
                     /locus_tag="VF516_35080"
     CDS             8011..8277
                     /locus_tag="VF516_35080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113024.1"
                     /translation="MDDWLRRTVYESQLPRRKPSAPADLQIRLRGLSCRRLVATMDPE
                     GDSFRVQIKNLRGDVASCESHDFINALEGAIKMAGARANRVEDA"
     gene            8951..12385
                     /locus_tag="VF516_35085"
     CDS             8951..12385
                     /locus_tag="VF516_35085"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113025.1"
                     /translation="MELSGRRLGEFTLREQIGEGGCAVVYRCEQPQLRRSAVVKVLHE
                     RGQRSDAALDRFRREAQLASQLDHPYAAHVYAFGDEDDGLFWIAMELVQGIALDNWLE
                     VHGPMPLAQFVPFFECVADVVHAAHERGIVHRDLKPSNVMVIERNGRLFPKLLDFGIA
                     KLIGELALPVPEHPAGTEAVTTAPLRTDWRRAQRTRTDPAAKDHRLTRTSAKLGSTAY
                     MSPEQWTNPQAVGAASDIYSLGILAYEVLTGRKPFTAESTHEYYHQHVYAEAPPLGDG
                     FPPEVDRVIRRALAKAPEARHGNALELATELRAALQLQPHEQLRSLAQVWDARARSPE
                     LLVQSKDLLRAPTAVVGELERAFVTESRRHAARRARLRRVLAATAVALALGAVWYPSR
                     LQTKLAEQEARAARQLAEATITQAELDQGRSSLIHDEPEAQLHLSRAYQRGGHSPSTA
                     FMLARALQPRLAEQARLASTAGRMWSAAFSPDGRQIVTTDDRAAQVWDSQTYRRTSVL
                     FHGDTVYEAVYSPDGTRIVTAGNDDTIKIWDSATGTLVRELRHDGARLRYFIAALSPD
                     GRLVAAIDTKGDVAHVWDAIAGILIAEIRNDGLGSPGIAFSADGRWLATTGGNDVRVL
                     DARTWRQATMIRGPRIHRLAFDPTGARLVTGATTGDAAIWAIPAGARIWHLCNGGESI
                     DAVAFSPDSRLVATGNRDGAVQVWRTESGELQSQLAPRRSKILAVEFDRASELLLAAN
                     ADGAVVVAEAGEGTPVAVLDGAPSILVAHFGPDSRRIVGASLEGTARVWDATPPYRRW
                     SSPPITDDCNLSPSSTSDGRFVAVGCQDHATRVWDTVRDQLVAELPATTLVAGDSPSA
                     YPAVSSGGDRTAIARGNTVEVYELPGGRLLRTVAHGAPVNAVAFAKTGRDLVSGATDG
                     SVIVTRDGGTRIVPPAAPSGVDAVGFLPDGRVVVSDAQRRLRVYDPGGAVLAEIEIPT
                     RVRSLRVGGNRIVGIPYTSKAAPSLLVDLERYRVVAELKGYFGRLLSARWTADGQILT
                     AGTDGTARLWDGTTGQLLQTYRGNSRFLADATLTTDGLVVAGGADGLLRFWDRDSGYL
                     LWTLPAHKSEIYGVHVEGGDIVTRGITGELARWTLPNPEQVIRACGDQERCAIVEP"
     gene            12382..12561
                     /locus_tag="VF516_35090"
     CDS             12382..12561
                     /locus_tag="VF516_35090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113026.1"
                     /translation="MKKTPRKLVLRSQTIRKLAEIDLTRAVGGVGNVQPPAETTDTHD
                     AACPTTLVVVATAAC"
     gene            complement(12558..>12916)
                     /locus_tag="VF516_35095"
     CDS             complement(12558..>12916)
                     /locus_tag="VF516_35095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113027.1"
                     /translation="RHGDRRHWPRRAPGARRRRSRGLRGPSAVAGSRLGSALGVEGGS
                     EGGRFGGEGKPGDNGVTMGGVIVGVIVIPEALKGAVDVLLLADAGDVTGAGAQAFKQL
                     GKEAARMSAAALRLRR"
BASE COUNT         1933 a         4627 c         4445 g         1911 t
ORIGIN      
        1 ctccgcgcgc gcgatgccgg cgccgggcgc gaccagcgcg aacaccagcg ccggggcgat
       61 gcgcatcagg gtgcggccgc atcggctgtg ccgtgctccg cccgcggcct ccggatccac
      121 gcgcggcagc atacacggcg accgcgccgc gcaatacccg ggggccgatc gatggacggc
      181 ccgcgcgagg tgcgacgagc gagtgcgaca cccggaatgt accggtcccc ggctcagcag
      241 gtcgatccct cgagcgtggg ggccggcggc gtccgctccg tcagggctcg atcgcagagc
      301 gccgtcaccg cccccagcgc gtacggcttg cccaggaagt acggtgtccc cacgcgcgcc
      361 gcgacctgct cgagatcgat gaccccggac agcaggatga tcgggatggt ctcgagtcca
      421 cagttgcgca cgaacatctg acaggccatc ccggggccgt cgaggatcgg catctcgacg
      481 tcgagcagca cggcgtccgg cagccggacg gagagcgcgc gcagcccgtc ctcgccgtcg
      541 cgcgcgatcc ggacctggtg accgtgatac acgagcgcct cgccgagcag ctcggccatg
      601 tcggggtcgt catcgacgat cagcagatcg gccatcacgc ctctggcgcg gcaccatcgc
      661 cccgtcggcc cggcgtccat gccccggatc gaccgggagg cgaaccgtga acgtcgaccc
      721 tgcgcccttc gcgctcgcga ccgtgatctc gccgtcgtgc gcttcgacga tccggcgcgc
      781 cacgaacagg ccgagcccgg cccccggggc ggcttgtacg gctcccccgc cgcgccggaa
      841 cgggtcgaac acgtgctcga ggtcgtccgc ggacatccct accccctcgt cggccacgga
      901 caccacgacg gtctccggcc gctcggcgac cgtgacccgg accgaaccac cgggcggcga
      961 gtacttgatc gcgttgttca ccaggttggc gaggacctgc tcgacccgca tcggatcgca
     1021 ccgcacgcgc accggctggt cgggcgcgct cacgatcagc gcgtgggtcg ccgtggcggg
     1081 ctcgaacagg tcgagcgtga tgcgcacgag ctcgcgcagg tcgcagtctt cgagccggag
     1141 gtcgaggtga cccgactcga tgcgagacgc gtcgaggaag tcgtagacca tccgctccag
     1201 gcgaccgatc tgccggctga tgcgtgcgac cagctgccgc acgcggggct cggacggcag
     1261 cgcctggtcg ggcgggatca tgtcggtcgc catcttcagc gctcccagcg ggttgcggat
     1321 gtcgtgcgcc accccggcga ggaacgcgag ctggtccgcg cgctggcgat cgagggtctg
     1381 cgccatgtcg ttgaagccgc gcgcgatcgt gcggaactcc tcgggctcct gctcgctcgc
     1441 gcgcgccgtc cggtcgccgc gcgtgtagcg atcgatcgcg cccgcgagct ggagcgccgg
     1501 ccggaacgtc ctgcgttgca gccaccaggc gagccccccg agccccacca cgagcagcag
     1561 gatcgcggcc acgccgatca cgtccgcgag ctcggcccag cgcgccgcgg tgcgctccgc
     1621 ggcgcgcccc tgctccacgt tgagctgcat ccactggcgc gccgacgcaa acgccggctc
     1681 gaacgccgcg cgcgtagcgt cccgcacctg cgcgggatcg gctgcgccgg ccgccgcacg
     1741 gtcgagcgca acgatgtact ggtcgacctg tcgcttcagg tcggagaaga tctccttgtc
     1801 ctgcgtgctg cccatgtagc cggccgcctc ggtcagccgg cgccggacca cgctctcggt
     1861 cgccccgcgc gcgagcgggt cggtggcctc gcgcagcgag atcagcccga gctccaggcc
     1921 ctcgccgacc cgcaggctct cgacctcggc gccgacctcc ttcgccgcgc ggtgcaggtt
     1981 cgtcgtcagg aggatcaacg cgatcgcgac cccgctgccg atgaccgcga ggatcgcagc
     2041 aaagaccaga aacgatcttc gcaggctcat gacatcttaa tacgacacac gcccccgggg
     2101 cagcacggcg aaacgaaccg ctcggcgagc gcgcaggccg aacaccgtcg ggaatttctc
     2161 gtgctgcgac cctaccgccg gactccggct gatcccgccg agcgagacgc tcgctcgaac
     2221 gcggcgtccg gcgatcacca tgagcgcccg ggaacgcgcg cccaggacca gggacccgag
     2281 ccggacggcc atgccccacc gcacaccgcc cgtctcgcag gccgatccgc ccccggagct
     2341 agaacgtcgc cgtgtagatc gtccccggac cgttgtacga cgtgccttcg ccccacacgc
     2401 cccaggtcgt cgtgccgtcg gtcgccatcg acgtgtagtc gccgtaggga tggtagaacc
     2461 cgcccggcgc gggcgtcgtg acctgatagc ggtgtgtcgt gggcagctgg actctcgcct
     2521 cgctcgtcca cgtcacgccg tccgtgctcg tgcgcgtgta gctgttgaac cgtccgcacg
     2581 cgccgggcgt gaccgagctg gtgcacgggt acttcacgcg gttgtcttgc cagatcacct
     2641 tcacgccggc ggccgtcgcc gcgatcgccg gaaacccgtg ccaggccccg atcggcgcgg
     2701 tcgacagctc cgcgccgctg ggatagctgc tcgcgctgta cgggatattc cagctcgcgc
     2761 cgagcgtatc ggagtacttg gtgtagatcc gctgtgcggc gcccgcgctc gtgcccgcgt
     2821 tgtagatcac gacgagccga tcgttgcccg ccctcggaat ggccgcgatc tgcacggcgg
     2881 ctccccagta gtcccagccc gcaccgacgt cgacgtcggc ggcgtacgag cgatcgatca
     2941 gcgacgtcgt gtacgtcgcg aagtggtcgc tcgagcgcgc cagccataga tacgaatcgt
     3001 gtttcgagtt gcccgtcacg tcggtccaca ccaggtaggt gttgccgtgc gagtcgcgga
     3061 tccccccggt ggcgagcgac gtgccgtggc cggcgcccgg cggcgggctc accttgatgt
     3121 gcgagaacgc ggtcgtcgtg gggtcgggcg agtacgcgac gtaggagctg ctcaggtgct
     3181 cgtacgcgac catcacaccc ccgccgggat cgacgagcag gacgtccttg tcgccgccgc
     3241 cgcgcgacgc gacgttgatc ggcgagctcc acgtcgcgcc gcgatcgtcg gagtagatca
     3301 gctcgatgtt cgtgttcgcc tgccgcccgt ccatgtacga cacgtacacc cggtggtgcg
     3361 ggccgacggc gacctgcaga tcgatctgcg tgcccgagac cggacagcgc gggaggatgt
     3421 acggctccca ggtcgcgccg ctgctcgtcg agcgctgaaa gtacgcgtac gacgtgacgc
     3481 ctcgggtcct gcacaccgcg ccgctcgaat cctgctgtgg cgcgccgatg tgcgcccacg
     3541 ccacgtacac gtaccggccg tcgaccgcga tcgccggctc ccagtcgtcg ccatcgctga
     3601 accccacctg cttgatcgcg atgtccgcac gcgcggacgc ggcaagcgcc accaccatca
     3661 cggacactcg gcctgccttc accacgggat ctatgacgcc ggtcgaacca cggagcagca
     3721 gcccacggcg cgaacgaccc ggcaaacagg agaaagcccg agacatgatc gagggcatgc
     3781 catcggctgc cgcagggttc caggccaaac tggaccggtg gacgcgacga tcacgagtct
     3841 ccagaccctg ctgctgctcg tcgccgtgct cgtggtggtc gccctcgtcg cgcagcggct
     3901 gaggctcgct ccgccgatcc tgatggtcat cgccgggatc gcgatggcgc tggtgcccgg
     3961 gctgccccgg ttcgagctgt cccccgagtt cgtgctcctg ttcgtcctcc cgccggtgat
     4021 ctactcggcc ggcgtatcga tgagctggcg cgagttccgg ttcaacctgc ggccgatcgg
     4081 gttgctcgcg atcggcggcg tcgtgttcac ggcgtgcgcg atcgccgccg tgctgcacta
     4141 cgcgctcggg ttcgcctggg agcttggctt cctgctcggc gcgatcgtcg caccgcccga
     4201 cgccctggcg ccgatctcga tcgcgcgaga cctcggcgtc ccgcggcgga tcctggtggt
     4261 gctcgagggc gaagggctcg ccaacgacgc gaccgccctg gtgctgtatc gcttcgcggt
     4321 cgcggcgatc tcgatcggcg cgttctcgct ggcgcgcgcg gtcggcgtgt tcgggctgat
     4381 cctcgccggc gagatcctct acggcgtcct gatcggctgg ctcagcctgc gactccggcg
     4441 ctgggcgcgc aacacccggg tcgagatcgc gctgtcgctg atgacgccgt atgtcgcatt
     4501 ctggccgtgc gcgcacctcg gaggttccgg cgtgctcgcg gccgtggcca ccgggctcta
     4561 cgtcagctgg aaaggtccac tgcagatctc gtcggcgacg cggctgcagg gcatcttctt
     4621 ctgggacctg accctctatc tgatcaacgg cctggtgttc ctctacaccg gcctccagct
     4681 gcgcacgctc gtcgagcgcg tgcatctcgg agagctgcac tggatgctgc tggccaccgc
     4741 gctgaccacg gcggtcgcga tcgccgcgcg cttcgtgtgg acctatccgg tggcctacct
     4801 tccgcgctgg ctcagcaagc agctcgcggc gcgcgatccg gcgcccgcat ggcaggtacc
     4861 gttcatgatc gcgttcaccg gcgttcgcgg cgtggtctcg ctcgccgccg cgctcgcgat
     4921 cccgacgacc ctgcgcgacg gccggccgtt tccgcatcgc gacacgttcc tgttcatcgc
     4981 gttcggcgtc atcctgatca ccctggtcgg ccagggcctg gccctgccgt ggctgatcca
     5041 caagctcggc ctcgcgcgcg gcggcgagga agagagccgg ctcgagctcg aacagcagcg
     5101 cgcggcccgc gagcaggtgc tcgacagcgc ggcgcgcgtg ctcgaccaga tcgccaacga
     5161 gcacacgctg ccggacgacg tggtgcgcca ggtccagacc cacatcgcga atcagcgccg
     5221 ccagatcccg agcgacctcg gagacagcct cgtgctcgcg cagtcggtgt ccgagctcag
     5281 ggccgagctg atcggcgagc agcgccgcgt gctgcacgag ctcctgcgcg gcggcaagct
     5341 cagcgacgag tcgcgccggc ggctcgagcg cgagctcgac ctcgaggaag agaccctgcg
     5401 ccatcgcacc gacctggcgc tgtagccccg atccgcgcgg gctcggcgca ccgcgcggcg
     5461 acgtgctgcc acggcttgcc ggactggccc aggtggcgag cgaaatgggg cggcctggcc
     5521 aggaaatcct ggagctgttc cttgatggcc tcacgaagtg agtccagacg gtcttgctcg
     5581 atcttttcga tcttcgctgc agcgccctgg tcgacgtcct tccgggcctt cctgaggctc
     5641 gcgctcgcgc tccgctcctt ctcgaacagc gtgcgcagct cgttcatcgt ccactccagg
     5701 ctgctcatgg catccccccg gcaccgcgcg cttcgagcgc agcaatgatg ggatcgagcc
     5761 aggcctgcgc cgtgccgcgc gcggccctgg cgtcgcgcag gaggcgcagg ttgcgcgcgg
     5821 tcgtctccgg ctcccagcgc tcgcgcacgg cggccagtgc gtcggccagt cgatccgccg
     5881 acgcgacctc gtcaccggcg aggacggcca gctcgaggag cgtcgcatgg tcccagtagt
     5941 cgggctcggt cgtgcgcagg cgctgctcga cggcgtaccg cacgacgggg accatgcggt
     6001 cgcgctccgc gagcgatctc ggcgagccgt ccagctcgag gagcgtgacg gcattgactc
     6061 cggggtatgc gtcgcgccag tcggcctcga aacctcgaac gtatgtatcg atggccttcc
     6121 tcaggtagcc ccgagcggtc agcgcgtccc ccgcgcgctg ggcctcgtcc cacagatcct
     6181 tgtagatccg gccgaggatg ccgagcgtct cggagctcgg cccgtgctgc tcgaccagct
     6241 cgttgagggt ctcgatcgcc ctgtcgcggt cggcgcgccg cgcaggatcc ttgcccgcgc
     6301 gacggttcag cgcgaacgcc agctgctcgc gcacgaggac ggtccggcgg agcacctccg
     6361 gcatgcggtc gtagagctgg atcatcgcgt cgtgcgcacc gaccgctcga tacgagagga
     6421 acaggtccac gaccacacct agctctgcgc cgtcgagcga cccgaggctc tgctcgatgt
     6481 cctcgagggc ttgcgcgccc gccctggcgt tcaggcggcg agcctcggtg aggcgcgcgc
     6541 gcagtgcctc ggacgcgcgg atgcggtcac gaaccaggtc ggtccgcagg cgcgcaagat
     6601 ccggtgaagg gtacccttcg aggagctgga acagcggact gtcgacggta gcctggtcgt
     6661 ggaagacctc gcgcagctcg cgcagccgtc gtgcgagcgc gacccggagc gccgcggctt
     6721 cgggcgcgcc gaaccggttg tccgtcccga tctcgtagga cagcgagcgc atcaagttga
     6781 cgtcgaacgg gagcgggctg tgcttcgcat agatcgccag tgtggtctgc gggcgcgtgg
     6841 cgtgccgcac gccgagctcg tagagcacgt tggcgttggc ggtcgtcagg tcggcgaccg
     6901 caaagtcaca gagcagaagc cgctcgaaca tcgccttgtg gatgatcccg cccgtgcgct
     6961 cgccgtcggc gcggagcggc tccagctcgg cgtcccggat cgcgggctcg atcgcctccg
     7021 aatagatccg gtcgaagtcg atatcggggc ggccggtggg atcgcgcttg gtaccgaacg
     7081 gcatcgccac gaagcacagg gggcgcaaga tggtcgacac gagcacctct ctcctcgggg
     7141 gtactatcgc atggtcgctc ggtcgccgtc tcgccatcgc ctcgggtggc catgccatct
     7201 tcctggtggt ggcctgaggc gagcgagcct gcgaccgcgc cgatcgccgt gacaccgcgg
     7261 ccaccgcgct gtagtgtatg cggctaatgg cgatcccgat gtgcgaaggg cagcggcaca
     7321 tcttcatcgt cggccgcgag ctgtgccggt gcggtcagga gcgatcgctg gtcctcgatc
     7381 cgcgggagac ccacatctcc gtcctcgtcg tcaagcagac gtggaagacg gtcgaggtgg
     7441 tgttctgctc acgcccgaat ggcgacggca tcgaggcgca gggctattgc ggacgctgcc
     7501 aaagccattc tttcccgctg tggcgatcac agcaaaggca tggaagcgga tggccacgca
     7561 gagccgcgag tcgagggaga tgcaacagga gctcgcagcc gacctcgcga gggcagcgaa
     7621 cgagagtccg tgcggatgcc gcgtcgcgtc cctgtgggat aagcagggtt cgaagtggtt
     7681 gctgtcatga gggctcagga cagcacacag gctgcgtctc cgaggacatt gcagcgccgc
     7741 agatggccgt tccccgtcga ggattgcagc ccactgcgcc cgccgacggc cgtttgattg
     7801 cgcgcacgtc gggccggaag tccggtccgc gctcgccgtc gcgcatcgtg cgccgcaaga
     7861 atgaagcgca cgcccctccg tcgccgcacc ccggcgctcg gacgagctca agcgggcgat
     7921 cgacgacacc gccgcgatag gggggctggc gttcgtgcac cgcgacctgg cccgcacgct
     7981 ctgcgtgacg cgagcgtcga gaacatgcac gtggacgact ggctgcgccg gaccgtgtac
     8041 gagtcgcagc tgccgcgccg caagccctcg gcgccggccg acctgcagat ccggctccgg
     8101 ggcctgagct gccggcgcct ggtcgcgacg atggacccgg agggcgacag cttccgcgtc
     8161 cagatcaaga acctgcgcgg cgacgtcgcg agctgcgagt cgcacgactt catcaacgcg
     8221 ctcgaaggcg cgatcaagat ggccggcgcc cgcgccaacc gcgtggagga cgcatgacga
     8281 ttcccttcga ggccaagagc atcgaggtcg accttgagca gccgcggcca agtccgagca
     8341 ggaccgccgc gaacaccgcc gactcgccgg tgacgtagcc gagctgctgg cgcacgacga
     8401 cgcgatgcgg gtgccgatcg cggtgcgctg gcttatagaa accggcgtcg aaccgatcgt
     8461 ggtggcgaag accctggcga ttggaggacg cgccgtccat tcaccgccaa cgatcgcgcg
     8521 tgggtgcggg accacgcggc gggatcgctc gccgagatcg caaagccacg ctgcggctgg
     8581 tcgcgctccg cacgtcgcgc aacctgtccg acgccggcgg caaggcttgg gatggcgccg
     8641 gtgtccctgg acaagtggct cggtcgccga aggtcgccgc tgatgattca gtcgggccgc
     8701 aggagccacc gatgagccag gccgcgacgg cagctcgagt agctccgcca agctggcggt
     8761 gcgggcggct gcctacacca gcagacagat cgaggaacgg acgctggtaa ggaacgtgag
     8821 ggtgcgagct ggtgcgctcg atgcagtcgc gagctgcacc tcacgcggat gccgcaaaat
     8881 gcgccggcga cgcctactcc cagtagatgg gcgacgccta gtctatcccc tcacggagga
     8941 gtcacagctg atggagctct caggacgcag gctgggcgag ttcaccctgc gcgagcagat
     9001 cggcgagggg ggctgtgcgg tcgtgtaccg ctgcgagcag ccacagctcc ggcgcagcgc
     9061 ggtcgtcaag gtgttgcacg agcgcgggca gcgcagcgac gctgcgctcg atcgctttcg
     9121 gcgcgaggcg cagctcgcat cgcaactcga tcacccctac gcggcgcacg tgtacgcgtt
     9181 cggcgatgag gacgacggcc tgttctggat cgcgatggag ctggtgcagg gcatcgccct
     9241 cgacaactgg ctggaggtgc acgggccgat gccgctcgcg cagttcgtgc cgttcttcga
     9301 gtgcgtcgcc gacgtcgtgc acgctgccca cgagcgcggg atcgtccacc gcgacctgaa
     9361 gccgtcgaac gtgatggtga tcgagcgcaa cgggcgcctg ttccccaaac tgctcgactt
     9421 cgggatcgcc aagttgatcg gcgagctcgc gctccctgtg ccggagcacc ccgcgggcac
     9481 cgaggcggtg acgaccgcgc cgctgcggac cgactggcgg cgcgcccagc gcacacgcac
     9541 ggaccccgcg gcgaaggatc atcgcctgac ccgcacgagc gccaagctcg gctcgacggc
     9601 gtacatgtcc ccggagcagt ggacgaaccc gcaggccgtc ggcgcagcca gcgacatcta
     9661 ctcgctcggc atcctcgcct acgaggtgct gaccggacgc aaaccgttca ccgccgagag
     9721 cacgcacgag tattaccacc agcacgtcta tgccgaggca ccgccgctcg gcgatggctt
     9781 tccacccgag gtcgatcgcg tcatccgacg cgcgctcgcc aaggcccccg aagctcgcca
     9841 cggcaacgcg ctcgagctgg ccacggagct gcgggcggcg ctgcagctgc agccgcacga
     9901 gcagctccga tcgctggccc aggtgtggga cgcccgcgcg cggtcgcccg agctgctggt
     9961 ccagagcaag gatctgctgc gggctcccac cgcggtcgtc ggcgagctcg agcgggcgtt
    10021 cgtgaccgag agccggcggc acgccgcgcg cagggcgcgc ctccgccggg tgctggccgc
    10081 gaccgcggtg gcgctcgcgc tgggagcggt ctggtatccg agcaggctcc agaccaagct
    10141 cgcggagcaa gaagcgcgcg cagcccggca gttggccgaa gcgaccatta cgcaggccga
    10201 gctcgaccag ggccgctcgt cgctgatcca cgacgagccc gaggcgcagc tccacctcag
    10261 ccgcgcctac cagcgcggcg gccactcgcc gagcacggcg ttcatgctcg cgcgagcgct
    10321 gcagccgcgc ctcgcggagc aggcgcgcct cgcgagcacc gccgggcgca tgtggtcggc
    10381 cgcgttctcg ccggacggcc ggcagatcgt cacgaccgac gaccgggccg cgcaggtctg
    10441 ggactcgcag acataccggc ggacctccgt gctgttccac ggcgataccg tctacgaggc
    10501 cgtatacagc cccgacggca cgcggatcgt gaccgccgga aacgacgaca ccatcaagat
    10561 ctgggactcg gccaccggca cgctcgtacg cgagttacga cacgacggtg cgcggctacg
    10621 ctacttcatc gccgcgctgt ctcccgatgg ccggctcgtc gccgcgatcg acaccaaggg
    10681 cgacgtcgcg cacgtctggg acgcgatcgc cggcatcctg atcgccgaga ttcgcaacga
    10741 cggcctagga tcgcccggca tcgccttcag cgccgacggt cgctggctcg ccacaaccgg
    10801 gggcaacgac gtgcgcgtgc tcgacgcacg gacgtggagg caggccacta tgatccgcgg
    10861 acctcgcatc caccgcctgg ccttcgaccc gaccggcgcg cgtctcgtca cgggcgcgac
    10921 aacgggcgac gcagcgatct gggcgatccc ggccggcgcg aggatctggc atctgtgcaa
    10981 cggcggcgag tcgatcgacg cggtggcgtt ctcacccgac agccggctcg ttgcgactgg
    11041 gaaccgcgac ggcgcggtgc aggtctggcg tacagaatca ggagagctgc agagccaact
    11101 cgccccccgc cgcagcaaga tcctcgcggt cgagttcgac agggcttccg agctcctgct
    11161 cgccgccaat gccgacgggg ccgtcgtcgt cgcggaggcc ggcgagggga cgccggtcgc
    11221 cgtgcttgac ggggcgccga gcatcctggt cgcgcacttc gggccggact cgcgccgtat
    11281 cgtcggcgca tccctagaag gaactgcccg ggtgtgggac gcaaccccgc cgtaccgccg
    11341 ctggagctcg ccgccgatca ccgacgactg caatctgtcc ccgagctcca cgtcggacgg
    11401 ccgctttgtc gccgtcggct gtcaggacca cgcgacccgc gtctgggaca ccgtgcgcga
    11461 ccagctggtc gccgagttgc ccgccacgac gctggttgcc ggcgactccc catccgcgta
    11521 cccagccgtg tccagcgggg gcgaccgcac ggcgatcgcg cgcggcaaca cggtggaggt
    11581 ctacgagctt cctggaggcc gactgctgcg cacggttgcg cacggcgcgc cggtcaacgc
    11641 ggtggcattc gcgaagaccg ggcgagacct cgtcagcggc gcaaccgacg ggtccgtgat
    11701 cgtcacacgc gacggcggca cgcgaatcgt gccccctgct gccccgagcg gcgtcgacgc
    11761 cgtgggcttc ttgcccgatg gtcgggtcgt tgtatcagac gcgcagcggc ggctgcgggt
    11821 ctacgatccc ggaggcgcgg ttctagcaga aatcgagatc ccgacgcggg tcaggtcgtt
    11881 gcgagtcggc ggcaatcgca tcgttggcat tccatacacc agcaaggcag ctccgtcgct
    11941 gctggtggat ctggagcgct accgagtcgt cgccgagctc aaggggtact tcggtcgact
    12001 cctctcggcg cgctggaccg cggacggcca gatcctcacc gcgggcactg acggaactgc
    12061 ccggttgtgg gatggcacga caggccaact cctccagacc taccgcggga actcgcgatt
    12121 cctcgccgac gcgacgctca cgaccgacgg cctagtggtg gccggcggcg ctgacgggct
    12181 gctgcggttc tgggacaggg acagcggcta tctgctgtgg acgctccccg cgcacaagtc
    12241 ggagatctac ggcgtccacg tcgagggcgg cgacatcgtg acgcgtggaa tcacggggga
    12301 actcgcgcgc tggacgctgc cgaaccccga gcaggtgatt cgggcgtgtg gtgaccaaga
    12361 gcgctgcgct atcgtcgaac catgaagaag acacctcgaa agctcgtgct gcgtagccag
    12421 accatccgca agctagctga gatcgacctc acgcgcgccg tcggaggagt cggcaacgtg
    12481 cagcctccgg cggaaaccac cgacacgcat gacgcggcct gcccgactac gctcgtcgtt
    12541 gtcgccaccg cggcttgcta gcggcgtaac cgcagcgcgg cggccgacat cctggcggcc
    12601 tccttgccaa gctgcttgaa cgcctgagca cccgcgccgg tgacgtcgcc cgcgtccgcg
    12661 aggagcagga cgtcgacggc gcccttgagc gcctcgggga tcacgatcac gccgacgatc
    12721 acgccgccca tcgtcacgcc gttgtcgccc ggcttgccct caccgccgaa ccggccgccc
    12781 tccgagccgc cctccacgcc cagcgccgaa ccgagccggc tccccgcgac cgcgctcggg
    12841 cctcggagcc ctcgtgatcg ccgccggcgc gcgcctgggg cccgccgggg ccagtgccgc
    12901 cgatcgccgt gtcggg
//
LOCUS       DASVOR010001891         4343 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_78263,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001891 DASVOR010000000
VERSION     DASVOR010001891.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4343
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78263"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(282..677)
                     /locus_tag="VF516_35100"
     CDS             complement(282..677)
                     /locus_tag="VF516_35100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX8113028.1"
                     /translation="MSETPAAELHHDDHSHGLSHVAPVKVLVATGGTLLLLTLVTVLA
                     TKVDFGANINLAIAMAIAVLKATLVILFFMHLRYDRLFHSVVFVAAILAAALFVGFTL
                     MDTNQYQQTNIWNPDAPPPAPIGPRPTSG"
     gene            complement(686..2011)
                     /locus_tag="VF516_35105"
     CDS             complement(686..2011)
                     /locus_tag="VF516_35105"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX8113029.1"
                     /translation="MSEQLHEPHTGDHGHAGEHGAHHGSPFIQHHYDDAQHQFDSGKL
                     GIWLFLAQEVLFFSALFVAYTLYRHHHPEIYSYAHKYLDVKYGAINTAVLIFSSLTAA
                     WAVRCAQLGQRRGLILCIALTIGCACTFLGIKYIEYSHKVHEHILFGRYFDPCVSSGG
                     RELLTRNNNCAGTKSTVVWDYGAGTPTAGCFNDIDQDPHTEGVQADCTVKEYSLTETP
                     RRVTQPKVDADGKPVVGPDGKPETEEVDRPDVTEHFTRDITERCVEEPLPGEEPANHE
                     AARTSKFPCWQPAFQPAVCKKGVGILVAYGDQEEREHVRIKAECKPAPKPADAGDLLA
                     DKDRPLQLGKPQIQPRHQLTVHEQHEIEAAGPPPEHTNMFFTIYFAMTGLHGIHVLFG
                     VGVFTWLLIRALKGHFTPDYFGPIDYAALYWHIVDLIWIFLFPLLYLIH"
     gene            complement(2008..3759)
                     /locus_tag="VF516_35110"
     CDS             complement(2008..3759)
                     /locus_tag="VF516_35110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8113030.1"
                     /translation="MATSKTQNQHGYPDGPTTEPDFLHVSRGVRSWMLTLDHKRIGMM
                     YLFGILGSLIVGGAFALLVRTELWAPGKTIVGQDTYNAFFTLHGAVMVFLVIIPGIPA
                     ALGNIMMPIQLGAPDVAFPRLNLASFYLWCAGALLLVASVPGGLDTGWTLYTPYSLET
                     KSPVLVATSGVFLLGFSSIFTGLNFLVTIHKFRPQGMGWFQLPLNVWAIYATAIMQVL
                     ATPVLGITVLLLAVERFGHIGIFDPTLGGDPVLFQHFFWFYSHPAVYIMIIPAMGVIS
                     ELVSTFARKPIFGYRFVAYSSVSLALLSFLVWGHHMFVSGQSRLANMVFSALTFTVGI
                     PSAIKVFNWVATLYKGDIRLKTPMLYALSFLLLFTIGGLTGLFLGILAVDVHLHDTYF
                     VVAHFHYVMMGSTLVAFMGALHYWWPKFTGRFYPESLGRVCALGVFFGFNLTFLPQFV
                     MGARGMPRRYWDYDPQYQIYHQLSTIGAFVLGTSLFVTVIYLIASFWNGKLAPRNPWG
                     GSSLEWQAPTPPPLYNFEKPPVLHELYNYDDLVEVEEDRWERQAPTEVEPSEAPGDAH
                     AHAAPEKPTGGDTGGQS"
     gene            complement(3771..>4343)
                     /locus_tag="VF516_35115"
     CDS             complement(3771..>4343)
                     /locus_tag="VF516_35115"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8113031.1"
                     /translation="NTPVRLVMTSKDVIHSFYAPVMRVKQDILPRRYTYAWFFPTKPG
                     TYRLTCAEYCGTNHSQMGITNDGRRAVLVVHEPGGYERYLADKAAQSTALPPEQLGKL
                     LYEKKGCNACHTTDGTPRVGPSWKGTFGTTVPLADGTSIKMDEDYIRESIFKPQAKSR
                     PGFPPSMPSFEGQLKEREIEGLIAFIKSLK"
BASE COUNT          720 a         1370 c         1571 g          682 t
ORIGIN      
        1 ggcgtcccac ggcgcgcagc ccgcggcggc gtcgcggccg tcccggtccg gagattccat
       61 gtgacatcgg ccagatggcg cggccgagcc ggtggccctg gaagtcgaca tccgcccatc
      121 gtgatcgggc cagagcgcac aggcaagcag cggccggccg agaaacggcc gcgcgtggat
      181 gcacggtccg gcgtgcgcct gggcacgggg tcgcgcgcag cgtcacgcgg accttccgtc
      241 acgcgaggct tgcggtgccc tcggaggcag cggcgcgtcc gctagccgga cgtcgggcgc
      301 gggccgatcg gggccggcgg cggggcgtcc ggattccaga tgttggtctg ctggtactgg
      361 ttggtgtcca tcagcgtgaa gccgacgaac agcgccgcgg ccaggatcgc ggcgacgaac
      421 accaccgagt ggaacagccg gtcgtagcgc aggtgcatga agaacaggat gaccagcgtg
      481 gccttgagca ccgcgatcgc catcgcgatc gcgaggttga tgttggcccc gaagtcgacc
      541 ttggtggcga gcacggtgac cagggtgagc agcagcagcg tgccgccggt cgcgaccagc
      601 accttgaccg gggcgacgtg cgacaggcca tgggagtggt cgtcgtggtg gagctcggcg
      661 gcgggggtct cggacatggg cgcctctagt ggatcaggta gagcagcggg aacaggaaga
      721 tccagatcag gtcgacgatg tgccagtaca gcgcggcgta gtcgatcggc ccgaaatagt
      781 ccggggtgaa gtggcccttg agcgcgcgga tcaggagcca ggtgaacacg ccgacgccga
      841 acagcacgtg gatgccgtgc aggccggtca tcgcgaagta gatcgtgaag aacatgttgg
      901 tgtgctcggg cggcggaccc gcggcctcga tctcgtgctg ctcgtgcacg gtgagctggt
      961 ggcgcggctg gatctgcggc ttgccgagct ggagcggacg atccttgtcg gcgagcagat
     1021 cgccggcgtc ggccggcttg ggcgccggct tgcactcggc cttgatccgg acgtgctcgc
     1081 gctcctcctg atcgccgtac gcgacgagga tcccgacgcc cttcttgcac accgcgggct
     1141 ggaacgcggg ctgccagcac gggaacttcg aggtgcgcgc ggcctcgtgg ttcgccggct
     1201 cctcgcccgg caagggctcc tccacgcacc tctcggtgat gtcgcgcgtg aagtgctcgg
     1261 tgacgtccgg cctgtcgacc tcctcggtct ccggcttgcc gtcggggccg accaccggct
     1321 tgccgtcggc gtcgaccttg ggctgggtca cccgcctggg cgtctcggtc aggctgtact
     1381 ccttgaccgt gcagtcggcc tggacgccct cggtgtgggg atcctgatcg atgtcgttga
     1441 agcagcccgc ggtcggcgtg cccgcgccgt agtcccacac cacggtcgac ttggtgccgg
     1501 cgcagttgtt gttgcgggtc aggagctcgc ggccgcccga gctgacgcac ggatcgaagt
     1561 agcggccgaa caggatgtgc tcgtggacct tgtgcgagta ctcgatgtac ttgatcccga
     1621 ggaacgtgca cgcgcacccg atggtcagcg cgatgcacag gatcaggccg cgccgctggc
     1681 ccagctgcgc gcagcgcacc gcccacgccg cggtcaggct ggagaagatc agcaccgcgg
     1741 tgttgatcgc gccgtacttc acgtcgaggt acttgtgcgc gtacgagtag atctcggggt
     1801 ggtggtggcg atacagggtg taggcgacga acagcgccga gaagaacagc acctcctggg
     1861 cgaggaacag ccagatcccg agcttgccgg agtcgaactg gtgctgggcg tcgtcgtagt
     1921 ggtgctggat gaacggcgag ccgtggtgcg cgccgtgctc gccggcgtgg ccgtggtcgc
     1981 cggtgtgcgg ctcgtgcagc tgctcgctca tgactgacct ccggtgtcac cgccggtcgg
     2041 cttctcgggt gcggcatgcg cgtgggcgtc gccgggcgcc tccgacggct cgacctcggt
     2101 cggcgcctgg cgctcccagc ggtcctcctc gacctcgacc aggtcgtcgt agttgtacag
     2161 ctcgtggagc accggcggct tctcgaagtt gtagagcggc ggcggggtcg gcgcctgcca
     2221 ctccagcgag ctgccgcccc acgggttgcg cggcgccagc ttgccgttcc agaacgacgc
     2281 gatcaggtag atcacggtga cgaacagcga cgtgccgagc acgaacgcgc cgatcgtcga
     2341 gagctggtga tagatctggt actgcggatc gtagtcccag tagcgccgcg gcatgccgcg
     2401 cgcgcccatc acgaactggg gcaggaacgt caggttgaac ccgaagaaca cgccgagcgc
     2461 gcacacccgg ccgagcgact cgggatagaa ccggccggtg aacttcggcc accagtagtg
     2521 cagcgcgccc atgaacgcga ccagggtcga ccccatcatc acgtagtgga agtgggcgac
     2581 cacgaagtag gtgtcgtgga ggtggacgtc gaccgcgagg atgccgagga acagcccggt
     2641 cagcccgccg atcgtgaaca gcagcaggaa cgacagcgcg tacagcatcg gcgtcttgag
     2701 ccggatgtcg cccttgtaga gcgtcgcgac ccagttgaac accttgatcg ccgacgggat
     2761 gcccacggtg aaggtcagcg ccgagaacac catgttggcg agccgcgact ggcccgacac
     2821 gaacatgtgg tggccccaca ccaggaacga cagcagcgcg agcgacaccg acgagtacgc
     2881 gacgaagcgg tagccgaaga tcggcttgcg cgcgaacgtc gagaccagct cgctgatcac
     2941 gcccatcgcc gggatgatca tgatgtagac cgcggggtgg gagtagaacc agaagaagtg
     3001 ctggaacagc accgggtcgc cgcccagcgt cgggtcgaag atgccgatgt ggccgaaccg
     3061 ctcgaccgcg agcagcagca cggtgatgcc gagcaccggg gtcgcgagca cctgcatgat
     3121 cgcggtcgcg tagatcgccc acacgttgag cggcagctgg aaccagccca tgccctgggg
     3181 gcggaacttg tggatcgtga ccaggaagtt gaggccggtg aagatcgacg agaacccgag
     3241 caggaacacg ccggaggtcg cgaccagcac cgggctcttg gtctccagcg agtacggcgt
     3301 gtacagcgtc cagccggtgt cgaggccgcc ggggaccgag gcgaccagga gcagcgcgcc
     3361 ggcgcaccac aggtagaacg acgccaggtt gagccggggg aacgcgacgt cgggcgcgcc
     3421 gagctggatc ggcatcatga tgttgccgag cgcggccggg atcccgggga tgatgaccag
     3481 gaacaccatc accgcgccgt gcagcgtgaa gaacgcgttg taggtgtcct ggccgacgat
     3541 ggtcttgccc ggcgcccaca gctcggtgcg caccagcagc gcgaacgcgc cgccgacgat
     3601 cagcgagccg aggatgccga acaggtacat catgccgatc cgcttgtgat cgagcgtgag
     3661 catccagctc cggacgcccc ggctgacgtg caggaagtcc ggctcggtcg tcgggccgtc
     3721 ggggtaaccg tgctggttct gggtcttgct ggtcgccatg gttgccctcg ctacttgagc
     3781 gacttgatga aggcgatcag gccttcgatc tcgcgttcct tgagctggcc ctcgaacgac
     3841 ggcatcgacg gcggaaagcc gggacgcgac ttggcctggg gtttgaagat cgactcgcgg
     3901 atgtagtcct cgtccatctt gatcgacgta ccgtcggcca gcggcaccgt ggtgccgaac
     3961 gtgcccttcc acgacgggcc gacccgcggc gtgccgtcgg tcgtgtggca cgcgttgcaa
     4021 cccttcttct cgtacaggag cttgccgagc tgctcgggcg gcagcgcggt cgattgcgcc
     4081 gccttgtccg cgaggtagcg ctcgtacccg ccgggctcgt ggacgaccag caccgcgcgc
     4141 cggccgtcgt tggtgatccc catctgcgag tgattggtcc cgcagtactc ggcgcaggtc
     4201 aaccggtagg tcccgggctt ggtcgggaag aaccaggcgt aggtgtagcg ccgcggcagg
     4261 atgtcctgct tcacgcgcat caccggcgcg tagaagctgt ggatcacgtc cttggacgtc
     4321 atcaccaggc ggaccggcgt gtt
//
LOCUS       DASVOR010001892         2197 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_370580,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001892 DASVOR010000000
VERSION     DASVOR010001892.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2197
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370580"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..796)
                     /locus_tag="VF516_35120"
     CDS             complement(170..796)
                     /locus_tag="VF516_35120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113032.1"
                     /translation="MKVTIAVDPDHPGGSWWRAARDAKHPPPELRPLLTGKATMIEVA
                     PKRARELRAWCAAFEGWDPEAAPLSFVGLVGRPPSPRRSEGARSLHVRLAPYELALLE
                     PLASAREQSLADLLLTSAVVEARREEARATDDGDGDETRAEVVDLDAIEVGDLLYTDL
                     RCLPRGHAERVTAVQRQHNLWRVELASGPPAWFGTGARVWRKRKRGRP"
     gene            980..1222
                     /locus_tag="VF516_35125"
     CDS             980..1222
                     /locus_tag="VF516_35125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113033.1"
                     /translation="MEARSMDAADPTQAQFAAYRAMWDYFNVTLFGGALRRVFLNFSR
                     RAKSLGFFAPERWESGKASEAGDTRGVGVAVGLRAA"
     gene            1545..1871
                     /locus_tag="VF516_35130"
     CDS             1545..1871
                     /locus_tag="VF516_35130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113034.1"
                     /translation="MAKHRNPRPKVSKQPSPDNFDTGILIHQLYEDLVEVEALAVTAD
                     EAVTELPPRPRGKYGRTLARLYTLVGKTSGRAQGALERGEELVAMRAAQLVARVPKRS
                     PARRAR"
BASE COUNT          324 a          808 c          744 g          321 t
ORIGIN      
        1 gacgcctcgg tcggcggcga gcccttgtgt tgcgtgacga cgtgccgagc gcgacgtcga
       61 cccgtcgacg ccggcgacct gtcccgccga gctcgggcca gttcacccat cgctgggcgg
      121 ccccgccatc accggcgagc cagcgtcgac ttgggcacgg ggaacgagat cacggccgtc
      181 ctcgcttccg cttccgccat actcgggcgc cggtgccgaa ccacgccggg ggcccgctcg
      241 cgagctcgac gcgccagagg ttgtgctggc gctgcaccgc ggtcacacgt tccgcgtggc
      301 cgcgtggcaa gcaccgtagg tccgtgtaga ggagatcccc gacctcgatg gcatcgaggt
      361 cgaccacctc agcgcgcgtc tcgtcgccat caccatcatc ggtcgccctc gcctcctcgc
      421 gccgcgcctc gaccaccgcg ctcgtgagga gcagatccgc gagcgactgc tcgcgggcgc
      481 tggccagggg ttcgagtagc gcgagctcgt acggggcaag cctgacatga aggctacgcg
      541 cgccctcgct gcgtcgcgga cttgggggtc gaccgaccag cccgacgaac gagagcggcg
      601 ccgcttcagg atcccagccc tcgaacgcag cgcaccaggc gcgaagctca cgcgcgcgct
      661 tgggtgccac ctcgatcatc gtcgccttgc ccgtcagcag ggggcgcagc tcgggtggtg
      721 ggtgcttggc gtcgcgcgcc gcgcgccacc atgacccgcc cgggtggtcc gggtcgaccg
      781 cgatcgtgac cttcatcagc tggataggta tcgcatccgc gcgtcccacg tcgacggttg
      841 acgcggctcg gtgcctgcca gacgcacgac ggggaatcca ccaacctccg cgcggagttg
      901 caacgcgctc ttttttagga acccaaattc ggttccggcg ccttgacatt ttaggctcct
      961 aaaactacac aggggaagca tggaggcgcg ctcgatggac gcggcggacc cgacccaagc
     1021 ccagttcgcc gcctaccgcg cgatgtggga ctacttcaac gtcacgctat tcggcggcgc
     1081 cctgcgccgg gtgttcctca acttctcgcg ccgcgccaag agccttgggt tcttcgcccc
     1141 cgagcgctgg gagagcggca aggccagcga ggccggcgac acccgcggtg tcggcgtcgc
     1201 ggtcgggctc cgggcggcgt gaccacgatg cgcgcctctg cgagacgaaa atgcgcgccc
     1261 ttcgctcgcg gccttgcgcc ccgcgcgttg ccaggtccgc tgcctcagcg gcctcgtcgc
     1321 ctctggtcgt tccagacggc aaaggccgtg gccccggggc cgtgccgtgc gtccctcggc
     1381 ggcacgacct ccctgcggtc cctcaccgcg agatggacgt tctcctcatt ctccgcgaca
     1441 cgcgttctgt tcgtcccttt ccgggacagg ccgcgccacc tcctggcgca gaaccaaagc
     1501 ggcccgtcgg tggctggaac ccgccgacga gcctaggagt cacgatggcg aagcatcgca
     1561 acccccgccc gaaggtatcg aaacaaccct cccctgacaa cttcgacacc ggcatcctga
     1621 tccaccagct ctacgaggac ctggtcgagg tcgaggccct cgcggtcacc gccgatgagg
     1681 cggtcacgga gctgccgccg cgcccgcgag gcaagtacgg ccgaaccctg gcccggctct
     1741 acacgctcgt cggcaagacc tccgggcgcg cacagggcgc gctcgaacgg ggcgaggagc
     1801 tggtcgccat gcgcgctgcc cagctggtgg cgcgggtccc caagcgctcg ccagcgcggc
     1861 gggcgcgctg acctgctacg gaccgaacgc cgcgccggag cgatgggctc ggcgcggcgt
     1921 cgccgttttc ggggtccggc tgctcgggcc cctggacgaa cgaaacgaac cgggaggagc
     1981 atcgtcctcc ccaggaagcg cgaagcttgg cgccgacccc ctcgcgcgcc ggcacagggc
     2041 gcgatcgggt gcgcccgcgc cttggcgagc gcgatctctg gcgatagctg ctcgggtctg
     2101 ctatcggcga acgacatggg tcagtcggag cagctcgccc aacgaacgtt tgccgaggag
     2161 accgagcgca cgacggccgg cgcggccggg tggcaag
//
LOCUS       DASVOR010001893         5202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_20963,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001893 DASVOR010000000
VERSION     DASVOR010001893.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20963"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="VF516_35135"
     CDS             <1..594
                     /locus_tag="VF516_35135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8113035.1"
                     /translation="TLRHGAVRVTTPMLYFFGFMFLFVFGGMTGVALATSSLDIHWHD
                     TYFVVAHFHFIMVGGTLTMYLAALHYWFPKMFGRMYPQGWSIVAATAVILGFIFTFTP
                     QFLLGNAGMPRRYWSYPAEYQWLHILSTAGASILAMGLLLNLVYLVWALFRGPKAERN
                     PWSSRSYEWLAPTPPPPHNFEAPLVIRRGPYDYHLPE"
     gene            604..1272
                     /locus_tag="VF516_35140"
     CDS             604..1272
                     /locus_tag="VF516_35140"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEX8113036.1"
                     /translation="MTTIEPGPAPARELVRAADVQFENLARQAHAVRFGIWVFLASEL
                     LLFAGLFALYTAYRTEYPAGFAAGVAHSELAIGTINTSLLIISSYTAASALRAIRRDE
                     RRAALGRLGVTLALGVAFLALKGLEYSHHAAEGALPGVYYAFRELPGYGGQLFFTLYY
                     FMTGLHVLHLAAAVLVLAWLASRVSRGRSTAAYHADLEAGVLYWHLVDCIWIFLYPLF
                     YLLT"
     gene            1291..1575
                     /locus_tag="VF516_35145"
     CDS             1291..1575
                     /locus_tag="VF516_35145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113037.1"
                     /translation="MTDRPVLSLDDATKHRIALGVVLVLQYLSVGLHFLGFDGYPVVA
                     LVIAGIMVVISASVFMDLRWSLPATRLVAVLAVLYLGLLCLGVAGDVSFR"
     gene            2132..4528
                     /locus_tag="VF516_35150"
     CDS             2132..4528
                     /locus_tag="VF516_35150"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HEX8113038.1"
                     /translation="MSLVVTARRRARLEGREAMRNSDDGKAAVATRYIYRVIVKFRDF
                     VELPYDESAEQQLTRLNIGPWDRITAAVPGARLTPLFTTVSPEQIRELVGRAVQLDRC
                     YRPPNFLTYFAVEYPAGADPEQLAKLLRSWRLVEFAYPDAGPTPPPVNASDDPRSINQ
                     DYLDAAPGGIDARYAWGLPGGDGTGIGIVDIEQGWTLNHEDLAAAGITLISGVNQAYF
                     GHGTSVLGELRAVDNTTGDVGIAPVASVRVNSQYRTAANYNTADVILNAVTTMAYGDV
                     MLLEAQTTIAGSYLPVEVELAPFDAIRLATALGITVIEAGGNGSQNLDNFTDGGGNHV
                     LRRGDPAFKDSGAIMVGAASSSAPHARLGFSNYGSRIDCYAWGENIDTTGDGWMGNLT
                     TTYTTSFGGTSGASPIIAGAAALVQGLAQAALAYRFSPGALRVILSSPTTGTASATPA
                     TDLIGVMPDLRAIITGNELNLAPDVYMRDFVGDAGDPHTAAVSASPDIIVRNSPVANP
                     QASFGAGSGTENSNTLSDDVEQGQDNFVYVRVLNRGGSPATAVSATVYWSPPATLVTP
                     DLWSLVGSVTIPNVPTGNLLTVSNAITWPAAAVPSPGHYCFVGIVSSARDPGPSPGDL
                     LVWDNFVRFVRANNNVTWRNFNVVNNVPPPGAPPPGLPPIGWIRLPFLFPGAPDLPRE
                     MALEIVARLPDRAMVYFEAPLWLLDRLSHCVPRVTVDPLVTIDPRAGMGRLRIRPHGV
                     QALGAAVLAAKSRTACALYVHIDPMDREHVFQIAARQLWANQEVGRITWLLSPLKHDR
                     "
     gene            complement(4684..>5202)
                     /locus_tag="VF516_35155"
     CDS             complement(4684..>5202)
                     /locus_tag="VF516_35155"
                     /inference="COORDINATES: protein motif:HMM:NF040438.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase domain-containing protein"
                     /protein_id="HEX8113039.1"
                     /translation="PPVSLRSIGWPDIPGVTYSGILDGLHRLDYGRAFDIDRESGIVE
                     EPPRTVGHAAYRVLVPRVNSDGNEVAGLRGVGLEVPLGTYTGWNLRRAGFAEGEECGL
                     TGSFIPFARTRAERTASGDPRPSLDERYGDHAGYVAAVRAAAARLVAARLMLQADADA
                     VIAQADASSVLR"
BASE COUNT          770 a         1853 c         1704 g          875 t
ORIGIN      
        1 acgctgcgcc acggcgcggt ccgggtcacc acgccgatgc tgtacttctt cggcttcatg
       61 ttcctgttcg tgttcggcgg gatgaccggc gtcgcgctcg cgacctcgtc gctcgacatc
      121 cactggcacg acacctactt cgtcgtcgcg cactttcact tcatcatggt cggcggcacg
      181 ctgaccatgt acctcgcggc gctgcactac tggtttccga agatgttcgg ccggatgtac
      241 ccgcagggct ggagcatcgt cgccgcgacc gcggtgatcc tcggcttcat cttcaccttc
      301 acgccgcagt tcctgctcgg caacgccggc atgccgcgcc gctactggag ctatcccgcc
      361 gagtaccagt ggctccacat cctgtcgacc gccggcgcga gcatcctcgc gatgggcctg
      421 ttgttgaacc tcgtctacct ggtgtgggcg ctgttccgcg gtccgaaggc cgagcgcaac
      481 ccgtggagct cgcgcagcta cgagtggctc gcgccgaccc cgccgccacc gcacaacttc
      541 gaggcgccgc tcgtgatccg ccgcggcccg tacgactacc acctgccgga gtgaacgccc
      601 gcgatgacca ccatcgaacc aggtccggca ccggctcgcg agctcgtgcg cgcggccgac
      661 gtccagttcg agaacctcgc caggcaggcg cacgcggtcc ggttcgggat ctgggtgttc
      721 ctcgccagcg agctcctgct gttcgccggg ctgttcgcgc tctacaccgc ctaccggacg
      781 gagtacccgg ccgggttcgc cgcgggggtc gctcactccg agctcgcgat cggcacgatc
      841 aacacctcgc tgctcatcat ctcgagctac accgcggcct cggcgctgcg cgcgatccgg
      901 cgcgacgagc ggcgggccgc gctcggccgg ctcggcgtca cgctcgcgct cggcgtggcg
      961 ttcctcgcgc tcaagggcct cgagtacagc caccacgcgg ccgagggcgc cttgcccggt
     1021 gtgtactacg cattccgcga gctgccgggc tacggcggcc agctgttctt cacgctgtac
     1081 tacttcatga ccggcctgca cgtcctgcac ctcgcggccg ccgtcctggt cctggcctgg
     1141 ctggcctcgc gcgtgtcgcg cggccggagc accgccgcgt accatgccga cctggaagcc
     1201 ggggtcctgt actggcacct ggtcgactgc atctggatct tcctctatcc gctgttctac
     1261 ctgctgacct gatctccacc ggatgacgcc atgaccgatc gccccgtcct ctccctcgat
     1321 gacgccacca agcatcgcat cgcgctcggc gtggtgctgg tcctgcagta cctgagcgtc
     1381 ggcctgcact tcctcgggtt cgatggctac ccggtcgtcg ccctggtgat cgccgggatc
     1441 atggtggtga tctcggcgag cgtgttcatg gacctgcgct ggtcgctgcc ggcgacccgc
     1501 ctggtcgcgg tgctggccgt gctgtacctc ggcctgctgt gcctcggcgt cgccggcgac
     1561 gtcagctttc gctgagtgcg gcatgcgtcg acgctgtaag cgctcaagga gtcgtgtcga
     1621 ggaccttcac cttttggtgg gacgcgcatc atcgcaatgc ttcgagagac ccagcgtcta
     1681 cgccggcacc cagcgacgct tgtctcttca acgtctggcg cgcctcgaat ttcgactcga
     1741 aagttgaggt cggcgtccag atcgctgacc tgctcgcatc gtcgctgcgc gtgcccagtg
     1801 ggtcgtacga cccaacgcca cgttccgtcg cggactatcg cgacggagcg gcgggggcga
     1861 cagacctgga gctcggcagg cccggcgagc tggccgcgct ggggcaaggg cgcgcaggcg
     1921 gcgctgggcg acgctcgtcg ccttcgtgcc cacatcaccc gacgatacgc atcgtcgacg
     1981 aacacgagct ggtcgagctc gccgggcgcg ccgtgctgtc gtcgccactt tgcagtgcta
     2041 gtaagatcgg ttcgccggac cacgcaggtg cctccacccg ctgagacagc gcttggctcc
     2101 cgttgcggag ccacctcctg caaggcacgt tttgagcctg gtagtgacgg cgcgtcggcg
     2161 cgcacgtctg gaagggagag aggcgatgag aaacagcgat gatggtaagg cagcggttgc
     2221 gacccggtac atttaccgcg tgatcgtgaa gttccgggac ttcgtggaac tcccgtacga
     2281 tgaatcggcc gagcaacagc tcacgcgcct caacatcggt ccgtgggacc gcatcacagc
     2341 ggctgtccca ggcgccaggc tgacgccgct gttcacgacg gtctcgccgg agcagatccg
     2401 agagctcgtc gggcgcgccg tgcagctgga tcggtgctat cgcccgccaa acttcctcac
     2461 ttacttcgcg gtcgaatacc ctgcgggcgc cgatccggag cagctcgcca aattgctgcg
     2521 cagctggagg ctcgtcgagt ttgcctatcc ggacgctggc ccgacgccgc cacccgtcaa
     2581 cgcgagcgac gacccgaggt cgatcaatca ggactacctc gatgccgcgc ccggaggcat
     2641 cgacgcgcgc tacgcgtggg gccttcccgg cggcgacggt accggtatcg ggatcgtcga
     2701 catcgagcaa ggctggacgc tgaaccacga ggatctcgcg gccgccggca tcacgttgat
     2761 ttcgggggtc aaccaggcct acttcgggca cggcacctcc gtcctgggcg agctgcgcgc
     2821 ggtcgataac accaccggcg atgttggcat cgcccccgtg gccagcgtcc gcgtcaactc
     2881 gcaatatcgc accgccgcaa actacaacac ggccgacgtg atcctcaatg ccgtcaccac
     2941 aatggcgtac ggcgacgtca tgctgctaga ggcgcagacc accatcgcag gttcgtatct
     3001 gccggtcgag gtggagctgg ccccgtttga cgcgatccgg ctcgcgaccg cgctcggcat
     3061 taccgtgatc gaggcgggag gcaatggctc gcagaacctc gacaacttca cggacggcgg
     3121 cggaaaccac gtgcttaggc gcggagatcc cgcgttcaag gactcgggcg cgatcatggt
     3181 cggcgccgcc tcgtcgtcgg cgcctcacgc gcggctcggc ttctcgaact atggaagtcg
     3241 catcgattgc tacgcctggg gcgagaacat cgacaccaca ggcgacggct ggatgggaaa
     3301 cctgacgacg acgtacacca cgagcttcgg cggaacctcc ggcgcgtcgc cgatcatcgc
     3361 gggcgccgcg gcgctggtgc agggcctcgc gcaggcagcg ctcgcctacc ggttcagccc
     3421 gggagcgctc cgtgtcatcc tgagctcgcc gacgaccggc acggcctcgg cgacgccggc
     3481 gaccgacctg atcggcgtca tgcctgacct gagggcgatc atcaccggca acgagctgaa
     3541 cctcgcgccc gatgtctaca tgcgcgactt cgtcggcgat gccggagatc cccacaccgc
     3601 agcggtgtcc gcgagcccgg acatcatcgt gcggaactcg ccggtcgcca acccacaggc
     3661 gtcgttcgga gcaggcagcg gaaccgagaa ctccaacacg ctaagcgacg acgtcgagca
     3721 gggacaggac aacttcgtct acgttcgcgt actgaaccgc ggaggctcgc cggcgaccgc
     3781 cgtgtcggca acggtgtact ggtcgcctcc cgcgacgctg gtcacgccgg acctgtggtc
     3841 actggtcggc tcggtgacga tcccgaacgt tccgaccggc aacctgctca cggtgtccaa
     3901 cgcgattacg tggcctgccg ccgcggtccc gtcgccgggt cactactgct tcgtcggcat
     3961 cgtgagcagc gctcgggatc cagggccgag cccgggcgat ctcttggtct gggacaactt
     4021 cgtccgcttc gtccgggcga acaacaacgt gacgtggcgg aacttcaacg ttgtcaataa
     4081 cgttccgcca ccgggcgcgc cgccgccggg cttgcctccg atcggctgga tccggctccc
     4141 gttcctgttt cccggcgcac ccgacctccc acgggagatg gcgctcgaga tcgttgcgcg
     4201 actgccggac cgcgcgatgg tgtacttcga ggcccccctc tggctgctcg accggctgag
     4261 ccactgtgtt ccgcgtgtca ccgtcgaccc gcttgtcacc atcgacccgc gggcggggat
     4321 gggccggttg cgcatccgtc cccatggcgt tcaagcgctc ggcgcggcgg tccttgccgc
     4381 gaaatcgcgg accgcgtgcg ctctctacgt ccacatcgat ccgatggacc gagagcacgt
     4441 gttccagatc gccgcccgtc agctctgggc gaaccaggag gtcggtcgga tcacatggct
     4501 gctgtccccc ctgaagcacg accggtgacg ggggccgacg taactgatcc gtgaactggg
     4561 cgacccagcc gccgtcgacc gcgatcgcct ggccggtgac gaacgacgcg gcgtccgagc
     4621 acagccatcg cgcggccgtc ggcgccacgt ccgccttggt gtcgtgcacg tcgaccccgc
     4681 gcgtcaccgc aggacgctgc tcgcatcggc ctgcgcgatc accgcatcgg cgtcggcctg
     4741 gagcatcagg cgcgcggcga ccaggcgcgc cgcggcggcg cgcaccgcgg cgacatagcc
     4801 cgcgtgatcg ccgtagcgct cgtccagcga cggccgcgga tccccgctgg cggtgcgctc
     4861 ggcgcgggtt cgcgcgaacg ggatgaagct gccggtcagg ccgcactcct cgccctcggc
     4921 gaagccggcg cgccgcaggt tccagccggt gtaggtgccg agcggtacct cgaggccgac
     4981 gccgcgcagc ccggcgacct cgttgccgtc gctgttcacg cgcggcacca gcacgcggta
     5041 ggcggcgtgg ccgacggtgc gcggcggctc ctcgacgatc ccgctctcgc gatcgatgtc
     5101 gaacgcgcgg ccgtagtcga gccggtgcag gccgtcgagg atgccggagt acgtgacccc
     5161 ggggatgtcc ggccagccga tcgagcgcag cgagacgggg gg
//
LOCUS       DASVOR010001894         1771 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_165671,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001894 DASVOR010000000
VERSION     DASVOR010001894.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1771
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165671"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(32..>1771)
                     /locus_tag="VF516_35160"
     CDS             complement(32..>1771)
                     /locus_tag="VF516_35160"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8113040.1"
                     /translation="LLVERKASAPHVIALAGGEPIELGPATAFGFAVAADDWSRVAMM
                     TSANEIAVPAERDGDPPRALARTEKAIAYLAISPRGDTVVVHDGITIWTLPFAGGPLR
                     ELAHYADKLTTVTWSPDGKTLAIGGHLPEILVIDTATGAQTVLRGHSDRIYTLAFSRD
                     GTRLLSASDDATARVWNLATHSALVLRGHDDDVYRARFSADERSVATSSLDGSARVWR
                     LDSPSTAVLVERDPIDDLWFAGDRLAVRTKSSLARWDLAAGRREEVASWARESDHLGY
                     GIASHDGSRVAIPNADGSLALRGAGHPAIALRGHRGLITHVEFARDDRAVFSSSSDGT
                     LRRWDAATGDSRVVLESTTPIRGFAVARDGRMVAQTGDLTYAIDPAGRVSKLGKGPAW
                     CISLAEFEPVSDRLVAQRCDNSLAIIDGNRMIELPTGGYAMARIAVSPDGRWMAGGLA
                     DRTIRLWSTETGRVVDVLRGHSDFVLDVAFSPDGRQLASASYDKTIRLWDLATRQHRV
                     LRGHTAEIKRVMWRDADHLVTGSIDGTVRLWDAPSLELPTAFDLARRLDAATTARIAL
                     DRPSTGLPPSRGT"
BASE COUNT          223 a          657 c          656 g          235 t
ORIGIN      
        1 caggtctaac acacagcgac cggcgccggg gtcaggtgcc gcgcgacggc ggcaggccgg
       61 tcgacggccg gtcgagcgcg atccgcgcgg tggtcgcggc gtcgagccgg cgcgccaggt
      121 cgaacgcggt cggcagctcc aggctgggtg cgtcccacag ccggaccgtg ccgtcgatcg
      181 agccggtcac caggtggtcg gcgtcgcgcc acatcacccg cttgatctcg gcggtgtggc
      241 cgcgcagcac ccggtgctgg cgggtcgcca ggtcccacag ccggatcgtc ttgtcgtagc
      301 tcgccgacgc gagctggcgg ccgtcgggcg agaacgcgac gtcgagcacg aagtcgctgt
      361 ggccgcgcag cacgtcgacg acccggccgg tctcggtgct ccacagccgg atcgtccggt
      421 cggccaggcc gcccgccatc caccggccgt cgggcgacac cgcgatccgg gccatcgcgt
      481 agccgccggt cggcagctcg atcatccggt tgccgtcgat gatcgccagg ctgttgtcgc
      541 agcgctgggc gaccagccga tcgctgaccg gctcgaactc ggcgagcgag atgcaccacg
      601 ccggaccctt gccgagcttg ctcacccggc ccgcggggtc gatcgcgtag gtcaggtcgc
      661 cggtctgcgc gaccatccgc ccgtcgcgcg cgaccgcgaa cccgcggatc ggcgtggtcg
      721 actccagcac gaccctgcta tcgccggtcg cggcgtccca ccgccgcagc gtgccgtcgg
      781 acgacgacga gaacacggcc cggtcgtcgc gcgcgaactc gacgtgcgtg atcaggccgc
      841 ggtggccgcg cagcgcgatc gccgggtggc ccgccccgcg cagcgcgagc gagccgtcgg
      901 cgttggggat cgccacgcgg ctgccgtcgt gcgatgcgat gccgtagccg aggtggtccg
      961 actcgcgggc ccacgacgcc acctcctcgc ggcgccccgc cgccaggtcc cagcgcgcga
     1021 gcgagctctt ggtgcgcacc gccaggcggt cgccggcgaa ccacaggtcg tcgatcggat
     1081 cgcgctcgac cagcaccgcg gtgctcggcg agtcgagccg ccagacccgc gcgctgccgt
     1141 cgaggctgga cgtcgcgacg ctgcgctcgt cggccgagaa cctcgcacgg tagacgtcgt
     1201 catcgtggcc gcgcagcacg agcgccgaat gggtcgccag gttccacacc cgcgcggtcg
     1261 cgtcgtcgct cgccgacagc aggcgggtgc cgtcgcggct gaacgccagg gtgtagatcc
     1321 tgtcgctgtg gccgcgcagc acggtctgcg cgccggtcgc ggtgtcgatc accaggatct
     1381 ccgggaggtg ccccccgatc gcgagggtct tgccgtcggg cgaccacgtg accgtggtga
     1441 gcttgtcggc gtagtgcgcg agctcgcgca gcgggccgcc ggcgaacggc agcgtccaga
     1501 tcgtgatccc gtcgtggacc accacggtgt cgccgcgcgg tgagatcgcg aggtacgcga
     1561 tcgccttctc ggtgcgggcg agcgcgcgcg gcgggtcgcc gtcgcgctcg gcggggaccg
     1621 cgatctcgtt ggccgacgtc atcatcgcca cgcgcgacca gtcgtcggcg gcgaccgcga
     1681 acccgaacgc ggtcgccggg ccgagctcga tcggttcgcc gccggccagc gcgatcacgt
     1741 gcggcgcgct ggccttgcgc tcgaccagca g
//
LOCUS       DASVOR010001895         3557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_370603,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001895 DASVOR010000000
VERSION     DASVOR010001895.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370603"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /gene="rpsH"
                     /locus_tag="VF516_35165"
     CDS             <1..182
                     /gene="rpsH"
                     /locus_tag="VF516_35165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEX8113041.1"
                     /translation="ITGMRRVSRPGQRTYVPAKQVPRVRNGLGIAILSTSQGVMTDKE
                     ARSKGVGGEVLCEVW"
     gene            186..737
                     /gene="rplF"
                     /locus_tag="VF516_35170"
     CDS             186..737
                     /gene="rplF"
                     /locus_tag="VF516_35170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEX8113042.1"
                     /translation="MSRIGRKPLEIPKGVTVSITNDSVSTKGPKGTLTLKRHKDIEIR
                     QAKDEDKRDVIVFDRKGNLGPQRAAHGLMRALVGNMLTGVTQGFTRQLEINGVGYKAE
                     IKGSKVVLSLGFSHPIEYQLPEGITAKVDKNLLILSGIDRQQLGAATAKIRSFRPPEP
                     YKGKGIKYVEETILRKAGKTAGK"
     gene            837..1217
                     /gene="rplR"
                     /locus_tag="VF516_35175"
     CDS             837..1217
                     /gene="rplR"
                     /locus_tag="VF516_35175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEX8113043.1"
                     /translation="MAGHIKYVNPKQRKRARRKVSIRLRLSGTSERPRLSVFRSARYV
                     YAQAIDDTTGRVLAAASELEEILKAAVAGKPKKERARAIGKAIGDKLVALHIKAVVFD
                     RNGYIYHGRVKEVADGARDAGLAF"
     gene            1226..1750
                     /gene="rpsE"
                     /locus_tag="VF516_35180"
     CDS             1226..1750
                     /gene="rpsE"
                     /locus_tag="VF516_35180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEX8113044.1"
                     /translation="MLINPEEVGELVDRVVHINRVAKVVKGGRRFSFSALVVVGDQHG
                     YVGAGLGKANEVPEAIRKGTDRAKRNLFKIPLIKGTIPHEIEGFFGAAQVLFRPASPG
                     TGVIAGGAVRPVLEVAGITDILTKSIGTSNKHNVIHATIVALKALRSVEQVARTRGKN
                     LSDFQGVRSAELRG"
     gene            1753..1938
                     /gene="rpmD"
                     /locus_tag="VF516_35185"
     CDS             1753..1938
                     /gene="rpmD"
                     /locus_tag="VF516_35185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEX8113045.1"
                     /translation="MALKLKVTQVRSGVGRSAQQNKTLEGLGLRGPHRSKVLEDTPAI
                     RGMIRKVAHLVVVEPAD"
     gene            2060..2536
                     /gene="rplO"
                     /locus_tag="VF516_35190"
     CDS             2060..2536
                     /gene="rplO"
                     /locus_tag="VF516_35190"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEX8113046.1"
                     /translation="MTISLHNLHPNPGSTKAKKRLGRGRGSGTGKTSGKGVKGQKARP
                     GHHGARLAFEGGQMPMPRRIPKRGFKNPFRIEAFPINVATLERVFDAGATVDLDALRA
                     KGLVPKLTEYVKILGEGEITKQLTIKAYRASVTAKEKVEKAGGTIEVAGSPDTGRA"
     gene            2551..>3557
                     /gene="secY"
                     /locus_tag="VF516_35195"
     CDS             2551..>3557
                     /gene="secY"
                     /locus_tag="VF516_35195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEX8113047.1"
                     /translation="MAGSIANIAKIPELRRRILFTLFILAVYRVGVFITVPGVNRVEM
                     SHVVTRGGTGSFLGMFNMFSGGALQQFSIFTLGIMPYVTSSIVMQLLTVVVPKLDQLN
                     KEGEQGRRRINQLTRYGTIIVAAVQSWVAAGYVESLSKNGAEVVANPGLGFHLLTVLT
                     MTTGTTVIMWLGELITDQGIGNGSSLIIFAGIVAAMPDAATRFLVGGGSAGAGGLTVL
                     FMVILVIATIAAICYFERAHRRIPVQYTKRQVGRKMYSGAQSYLPLKINVSGVIPPIF
                     ASSILMFPAQLANMSGSRIMQEFSSVLHPADWRYNAIFTVLNVFFAFFYTSVVFNPVD
                     VA"
BASE COUNT          626 a         1228 c         1157 g          546 t
ORIGIN      
        1 cgatcaccgg catgcgccgg gtgtcgcggc cgggccagcg cacctacgtc cccgccaagc
       61 aggtgccgcg ggtccgcaac ggcctcggca tcgcgatcct gtccacgtcg cagggcgtga
      121 tgaccgacaa ggaagcccgg agcaagggcg tcggcggcga agtcctctgc gaggtctggt
      181 gaaccatgtc gcgcatcggt cgtaaaccgc tggagatccc caagggcgtc accgtgtcga
      241 tcacgaacga cagcgtgtcg accaaggggc ccaagggcac cctgacgctc aagcgtcaca
      301 aggacatcga gatccgtcag gccaaggacg aggacaagcg cgacgtgatc gtgttcgacc
      361 gcaagggcaa cctcggcccg cagcgcgcgg cgcacggcct gatgcgcgcc ctggtcggca
      421 acatgctgac cggcgtgacc cagggcttca cgcgccagct cgagatcaac ggcgtcggct
      481 acaaggccga gatcaagggc agcaaggtgg tgctgtcgct gggcttctcg cacccgatcg
      541 agtaccagct gcccgagggc atcacggcca aggtcgacaa aaatctattg atcctctcgg
      601 ggatcgaccg ccagcagctg ggggccgcga ccgccaagat ccgcagcttc cggccgcccg
      661 agccctacaa gggcaagggc atcaagtacg tcgaggagac catccttcgc aaggcgggca
      721 agaccgcggg caagtaacgt gatcgcacag gtcgtcgcgc ccggaccggc tgcccggcta
      781 ctgtagcccc ggccgtccgc caccgcgaca gcggcctcga tcaggagaat ggacacatgg
      841 ctggccacat caagtacgtc aaccccaagc agcgcaagcg agcccggcgc aaggtgtcga
      901 tccgcctgcg gctgtccggg acctccgagc ggccccggct gtcggtgttt cgctcggcgc
      961 gctacgtcta cgcccaggcg atcgacgaca cgaccggccg cgtgctcgcg gccgccagcg
     1021 agctcgagga aatcctcaag gccgcggtcg cgggtaagcc caagaaggag cgcgcccgcg
     1081 cgatcggcaa ggcgatcggc gacaagctcg tggctctcca catcaaggcg gtcgtgttcg
     1141 accgcaacgg ctacatttac cacggccgcg tcaaggaggt cgccgacggc gcccgtgacg
     1201 ccggcctcgc gttctagagg acgacatgct gatcaatccc gaagaagtag gcgagctcgt
     1261 cgaccgcgtc gtccacatca accgcgtggc caaggtggtc aagggtggcc ggcggttctc
     1321 gttctcggcg ctcgtggtgg tcggtgacca gcacggctac gtcggcgccg gtctcggcaa
     1381 ggccaacgag gtgcccgagg cgatccgcaa gggcaccgac cgcgccaagc gcaacctgtt
     1441 caagatcccg ctgatcaagg gcacgatccc gcacgagatc gagggcttct tcggcgcggc
     1501 gcaggtgctg ttccggccgg cgtctcccgg caccggcgtg atcgccgggg gtgcggtccg
     1561 gccggtgctc gaagtcgccg ggatcaccga catcctgacc aagtcgatcg gcacctcgaa
     1621 caagcacaac gtcatccacg cgacgatcgt ggcgctcaag gcgctgcgct cggtcgagca
     1681 ggtggcgcgg acccggggca agaacctgtc ggacttccag ggcgttcggt ccgccgagtt
     1741 gcgaggttag tcatggcgct caagctcaaa gtcacccagg tccgctcggg ggtcggccgc
     1801 tcggcgcagc agaacaagac cctggaaggc ctcggccttc gcgggccaca ccgctccaag
     1861 gtcctcgagg acaccccggc gatccgcggc atgatccgca aggtcgcgca cctggtggtc
     1921 gtcgagccgg cggactagcg tccgctcgtc gtttatttcg cccccggctc gcctccggct
     1981 cgtcgccgcg atttcatccc cagcagttgc gctcactcaa tttccgcgcc agattgcaag
     2041 cgcggtcggg aaagccccca tgaccatttc actccacaac ctccacccga acccgggctc
     2101 gaccaaggcg aagaagcgcc tcggccgcgg tcgtggctcg ggcaccggca agacctccgg
     2161 caagggcgtc aagggccaga aggcgcgccc ggggcaccac ggcgcccggc tcgcgttcga
     2221 gggcggccag atgccgatgc cgcgccggat ccccaagcgc gggttcaaga acccgttccg
     2281 catcgaggcg ttcccgatca acgtcgcgac cctcgagcgg gtgttcgatg ccggcgccac
     2341 cgtcgatctc gacgcgctgc gcgccaaggg gttggtcccc aagctcaccg agtacgtgaa
     2401 gatcctcggc gaaggtgaga tcaccaagca gctcacgatc aaggcgtatc gcgcatcggt
     2461 caccgccaag gaaaaggtcg agaaggccgg cgggacgatc gaggtcgccg ggtcaccgga
     2521 tacaggccgg gcataaccac aagggccgac gtggcaggtt cgatcgcaaa catcgctaag
     2581 atcccggagc tccgccgccg gatcttgttc acgttgttca tcctcgcggt ctatcgcgtc
     2641 ggcgtgttca tcaccgtccc cggggtgaac cgcgtcgaga tgagccacgt cgtgacccgc
     2701 ggcggcacgg gctcgttcct cggcatgttc aacatgttct cggggggcgc gctccagcag
     2761 ttctcgatct tcacgctcgg catcatgccg tacgtgacct cgtcgatcgt gatgcagctg
     2821 ctcacggtcg tggtgcccaa gctcgaccag ctcaacaagg aaggcgagca gggccggcgc
     2881 cggatcaacc agctgacccg ctacggcacg atcatcgtcg ccgcggtcca gtcgtgggtc
     2941 gcggccggct acgtcgagag cctgtcgaag aacggcgccg aggtcgtcgc caacccgggc
     3001 ctcgggttcc acctgctcac cgtgctgacc atgacgaccg gcacgacggt catcatgtgg
     3061 ctcggcgagc tgatcaccga tcagggcatc ggcaacggct cgtcgctgat catcttcgcc
     3121 ggcatcgtcg ccgcgatgcc ggacgcggcg acccggttcc tcgtcggcgg cggatcggcc
     3181 ggcgcgggcg ggctgaccgt gctgttcatg gtgatcctcg tgatcgcgac gatcgcggcg
     3241 atctgctact tcgagcgcgc gcaccgccgg atcccggtcc agtacaccaa gcgccaggtc
     3301 ggccggaaga tgtactccgg cgcgcagagc tacctgccgc tcaagatcaa cgtctcgggc
     3361 gtgatcccgc cgatcttcgc gtcgtcgatc ctgatgttcc ccgcccagct cgccaacatg
     3421 tcgggctcgc ggatcatgca ggagttctcg tcggtcctgc accccgcgga ctggcgctac
     3481 aacgcgatct tcaccgtgct gaacgtgttc ttcgcgttct tctacacctc ggtcgtgttc
     3541 aacccggtcg acgtcgc
//
LOCUS       DASVOR010001896         1774 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_370607,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001896 DASVOR010000000
VERSION     DASVOR010001896.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1774
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370607"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1774)
                     /locus_tag="VF516_35200"
     CDS             complement(<1..>1774)
                     /locus_tag="VF516_35200"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113048.1"
                     /translation="PGTRVEHYEIIRALGRGGMGEVHLARDVRLGRLVALKFLIPSSP
                     EIARTLLIEARATAKCKHENIVVIHDMNEHRGMPYLVLEYLEGKSLNRLHAESPLPLI
                     RLIEVMIAVVRALDHAHRAGIVHRDLKPDNIFVTTAGDVKVLDFGIAKLVGSPASHAA
                     QTPETPALHDDETVITISSNGPVGTRPYMAPEQWLAVDIDHRTDLWAVGVILFRLVTG
                     KFPFELEGAALMYAVASLTEPVRSVRSLIPDFHPELAAIIDRCLRKVKDERFASAREL
                     LDALEPLLPHPVAGGGDDRCPYPGLMSFQEADADRFFGRDDQIARVVGRLATQSLIAI
                     VGPSGAGKSSFVRAGIVPALKREGPWATIAMRPGRSPLLSLAALSDPANAHNPDHVRA
                     VAQHMFAEPGYLGAVLRWRAASHGCRVLLYIDQFEELYTLVRDPGERAAFVTCLAAAA
                     DDSSSPVRVVLSLRSDFLDRAAEDRAFMARLTDGMHDLMPLGPDGLHQALVRPVEQAG
                     HRFESVELVSRMVADVVMAPGALPLLQFAAARRWEARDRPRRLMTTASYLAMGGIAGA
                     LAVHADDVLAELSPGRRRLAQAVFQ"
BASE COUNT          258 a          637 c          646 g          233 t
ORIGIN      
        1 gctggaacac cgcctgcgcc agccggcggc gccccggcga cagctcggcg agcacgtcgt
       61 cggcgtgcac cgcgagcgcg ccggcgatcc cacccatcgc gaggtagctc gcggtcgtca
      121 tcagccggcg cggccggtct cgcgcctccc acctccgggc cgccgcgaac tgcagcagcg
      181 ggagcgcgcc gggcgccatc acgacgtccg cgaccatccg gctgaccagc tcgacggact
      241 cgaaccggtg accggcctgc tcgacgggcc gcaccagggc ctggtgcagc ccgtcggggc
      301 cgagcggcat caggtcgtgc atgccgtcgg tcaggcgcgc catgaacgcc cgatcctcgg
      361 cggcgcgatc gaggaaatcg gagcgcagcg acagcaccac ccggaccgga ctcgacgagt
      421 cgtcggccgc ggccgcgagg caggtcacga acgccgcgcg ctcgcccgga tcgcggacca
      481 gcgtgtagag ctcctcgaac tgatcgatgt agagcagcac gcggcagccg tggctggcgg
      541 cgcgccagcg caggaccgcc ccgaggtagc cgggctccgc gaacatgtgc tgcgcgaccg
      601 cgcggacgtg atcggggttg tgggcgttcg cgggatcgct cagcgcggcg agcgacagca
      661 gcggcgagcg acccggccgc atcgcgatcg tggcccacgg accctcgcgc ttgagcgccg
      721 gcacgatccc ggcgcggacg aacgatgact tgcccgcgcc ggacggcccg acgatcgcga
      781 tcagcgactg ggtcgccagc cgcccgacca cccgcgcgat ctggtcgtcg cggccgaaga
      841 accggtccgc gtcggcctcc tggaacgaca tcagcccggg gtacgggcag cggtcgtcac
      901 cgccgccggc caccggatgc ggcagcagcg gctcgagcgc gtcgagcagc tcgcgcgcgc
      961 tcgcgaaccg ctcgtccttg accttgcgca ggcagcggtc gatgatcgcg gcgagctcgg
     1021 ggtggaagtc ggggatcagc gaccgcacgc tgcgcaccgg ctcggtcagc gacgccacgg
     1081 cgtacatcag cgccgcgccc tcgagctcga acgggaactt gccggtcacc agccggaaca
     1141 ggatcacgcc gaccgcccac aggtcggtgc gatggtcgat gtcgacggcg agccactgct
     1201 ccggcgccat gtacggccgg gtgccgaccg ggccattgct gctgatcgtg atcacggtct
     1261 cgtcgtcgtg gagcgccggg gtttccggcg tctgcgccgc gtggctcgcc ggactgccca
     1321 ccagcttggc gatgccgaag tcgaggacct tcacgtcccc cgcggtggtg acgaagatgt
     1381 tgtcgggctt gagatcgcgg tggacgatgc cggcgcggtg ggcgtggtcg agcgcgcgga
     1441 ccaccgcgat catcacctcg atcagccgga tcagcggcag cgggctctcg gcgtgcagcc
     1501 ggttgagcga cttgccctcc aggtactcga gcaccaggta cggcatgccg cggtgctcgt
     1561 tcatgtcgtg gatcacgacg atgttctcgt gcttgcactt cgccgtggcg cgggcctcga
     1621 tgagcagcgt ccgcgcgatc tcggggctcg acgggatcag gaacttcagc gcgaccaggc
     1681 ggccgagccg gacatcgcgc gcgaggtgga cctcccccat cccgccgcgg ccgagcgcgc
     1741 ggatgatctc gtagtgctcg acgcgggtgc cggg
//
LOCUS       DASVOR010001897         4470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_195432,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001897 DASVOR010000000
VERSION     DASVOR010001897.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195432"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="VF516_35205"
     CDS             complement(<1..253)
                     /locus_tag="VF516_35205"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113049.1"
                     /translation="MLSVADAARAVIARVPRLPGELVPLDQAAGRILAADIVAPRALP
                     GFDNSAMDGYAARSADLPGTLPVAGGVSAGELDVPALPAG"
     gene            406..3207
                     /locus_tag="VF516_35210"
     CDS             406..3207
                     /locus_tag="VF516_35210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8113050.1"
                     /translation="MQLADEHFRLLVESVADYAIYLLEPDGTIRSWNAGAQRLKGYTV
                     SEIVGHRFAQFFTPEDRESGKPARLLAAALRDGRIEDIGWRVRKDGTRFWASAVITAL
                     RDRAGEHIGFAKVTRDLTDRAYRAFIEATHAIVWTADATGQPNADSPSWREFSGQTET
                     EWREQHAWDVIHPADREPFREVWDHAKQTGTAFVAEYRLRRRDGVYVWVEGRAIPFFD
                     GEGRVREWFGVTLDISARKQAELETQRALALWTTTLRSIGDAVISTDAHGRVRFMNPV
                     AERLTGWQGDEAAGRPLIEVFPIFNEETGAVVENPVDKVLRGGVVVGLANHTVLRRRD
                     GTEIPIDDSAAPIHDPEGPPGAVPGPVIEGVVLVFRDASEEKRELYRRSFLATATEQL
                     LEADDHRDALTRIAQLAVPRLADWVGVNIAAPGATRAQPLTVAHADPVKVELARALAR
                     RYPLDPDAPAGVARVMRTGRSEFYPEIPAELLDQGTADDEHRRILRELDLRSALVVPL
                     RGKGPVFGAISFVLAGSNRRYTRRDLELAEDLARRAGLIIERRRLEEEAERAHRMKGE
                     FLATMSHELRTPLQAILGYASMLERGIARDPAKATAAILRNATAQTRLVEDILDVSRI
                     SSGKLQLAMTRVDIVAAIRAALESIRPAALARRIQISDSLPPDLGDVLGDSDRLQQVV
                     WNLLSNAVKFTPMDGVIEVRAERSGSTLRVIVRDTGKGIAPEHLTTIFERFRQVDSST
                     TREHRGLGLGLAIVRYLVEAHGGTVHAESPGPGQGATFIVTLPVRTDALAAAVRSHDS
                     RRPLDAERLRGIRILLVEDDDEARELLADVLAETGATVAQAASAADGMACLIAEPPHV
                     LISDIGMPFEDGNSFLRRVRALPPERGGDVPAIALTAYARPEDRRATEEAGFQLHVVK
                     PVRPDQLLAAIESLVQIRH"
     gene            complement(3224..4144)
                     /locus_tag="VF516_35215"
     CDS             complement(3224..4144)
                     /locus_tag="VF516_35215"
                     /inference="COORDINATES: protein motif:HMM:NF020269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GON domain-containing protein"
                     /protein_id="HEX8113051.1"
                     /translation="MGGRLAFTALWLTACGFNAQPGLPGDAASPPDAPIDTPPAVTCA
                     NLTCDPNATCAMTNTAKCICKSGFMGDGLSCTDINECATSNGGCPAACMNTPGNFTCY
                     TPTSCGDIKSHVSGAADGTYTLYLGGDARKPWQAHCAGMANNPREYLSLTGANFAQYT
                     HGGNSPGTDVKTTFTKVRFDPATMKIDSSDRSFATSTGMLDHSGSGTMVTSMPYGVAM
                     DCIGNNSAAGVAQIDLTGTSFTLSSGNMFIKAGNHAGGNVQVSPDNLHATINGGGDCG
                     WAGPMNIPSNPFNNTVTSANGTLLQLSYAP"
     gene            complement(4219..>4470)
                     /locus_tag="VF516_35220"
     CDS             complement(4219..>4470)
                     /locus_tag="VF516_35220"
                     /inference="COORDINATES: protein motif:HMM:NF016830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HEX8113052.1"
                     /translation="GLAAVSNGLSDDHRRYLALGGKGFLLGDGRLRYGRETIVETYYT
                     ARLHRGTFVAADLQLIARPGYNRDRGPILVGSLRFHLEL"
BASE COUNT          651 a         1552 c         1641 g          626 t
ORIGIN      
        1 ccccggccgg cagcgccggc acgtcgagct cgccggcgga gacccccccg gcgaccggca
       61 gcgtccccgg cagatccgcg gagcgcgccg cgtagccgtc catcgccgag ttgtcgaacc
      121 cgggcagcgc gcgcggtgcg acgatgtcgg ccgcgaggat ccggccggcc gcctggtcca
      181 gcgggacgag ctcgccgggc aggcgaggga cacgagcgat cacggcacgc gcggcatcgg
      241 cgacagacag cacgccggca tcttacgtcg ccgcggcgcg cggcgcctgc gaggacggcg
      301 aattggcaag agcccgagcg tgggcgcacg gcgagcgcga ggaaaaaaag ttcagcgcgc
      361 ctacacgtga caaccgtgcc gtgaacgcgg caacgtgcag tccgaatgca actcgcggac
      421 gagcacttcc ggctgctggt cgagtcggta gcggattacg cgatttatct gctcgagccg
      481 gatggcacga tccgttcctg gaacgccggc gcacagcgct tgaagggcta tacggtctcg
      541 gagatcgtgg gccatcgctt tgcgcagttc ttcacgccgg aggatcgcga gagcggcaag
      601 ccagcgcggc tgctcgccgc ggcgctgcgc gacggccgga tcgaggacat cgggtggcgg
      661 gtccgcaagg acggcacgcg gttctgggcg agcgccgtga tcacggcgct gcgcgatcgc
      721 gccggggagc acatcggctt cgccaaggtc acgcgcgatc tcacagaccg cgcctaccgg
      781 gcgttcatcg aggcgaccca tgcgatcgtg tggacggccg acgcgaccgg ccagcccaac
      841 gcggactcgc cgagctggcg cgagttctcc ggccagaccg aaacggaatg gcgcgaacag
      901 cacgcctggg atgtgatcca cccggccgac cgcgagccgt tccgcgaggt ctgggaccat
      961 gccaagcaga ccggcaccgc gttcgtggcc gagtatcgcc tgcggcggcg cgacggcgtg
     1021 tacgtctggg tcgagggccg cgcgatcccg ttcttcgacg gcgagggccg ggtccgcgag
     1081 tggttcggcg tgacgctcga catctcggcg cgcaagcagg ccgagctgga gacccagcgc
     1141 gcgctcgcgc tgtggaccac gacgctgcgc agcatcggcg acgcggtgat ctcgaccgac
     1201 gcgcacggcc gcgtccggtt catgaacccg gtcgcggagc ggctcaccgg ctggcagggc
     1261 gacgaggcgg ccggccggcc gctgatcgag gtgttcccga tcttcaacga ggagaccggc
     1321 gcggtggtcg agaacccggt cgacaaggtg ctgcgcgggg gcgtggtggt cgggctcgcc
     1381 aaccacacgg tgctgcgccg gcgcgacggc accgagatcc cgatcgacga cagcgcggcg
     1441 ccgatccacg acccggaggg gccgccgggg gccgtgccgg ggcctgtgat cgagggcgtc
     1501 gtgctggtgt tccgcgacgc cagcgaagag aagcgcgagc tgtaccgccg cagcttcctg
     1561 gcgaccgcga ccgagcagct gctcgaggcc gacgaccacc gcgacgcgtt gacccggatc
     1621 gcgcagctcg ccgtgccgcg gctggccgac tgggtcgggg tcaacatcgc agcgccgggg
     1681 gccacccggg cccagccgct cacggtcgcg cacgccgatc cggtgaaggt cgagctcgca
     1741 cgcgcgctcg cccggcgcta cccgctcgat cccgacgcgc cggccggcgt cgcccgggtg
     1801 atgcgcaccg gccgctccga gttctacccc gagatcccgg cggagctgct cgaccagggc
     1861 acagcggacg acgagcaccg ccggatcctc cgcgagctcg acctgcgatc cgcgctggtc
     1921 gtgccgctgc gcggcaaggg cccggtgttc ggggcgatct cgttcgtgct cgccggctcc
     1981 aaccggcgct acacccggcg cgatctcgag ctcgccgaag acctcgcgcg ccgcgccggc
     2041 ctgatcatcg agcgccggcg gctcgaggag gaggccgagc gcgcccaccg gatgaagggc
     2101 gagttcctcg ccaccatgag ccacgagctg cggacgccgc tccaggcgat cctcggctac
     2161 gcctcgatgc tcgagcgcgg catcgcgcgc gaccccgcca aggcgaccgc cgcgatcctg
     2221 cgcaacgcga ccgcgcagac ccggctggtc gaggacatcc tcgacgtgtc gcggatctcg
     2281 agcggcaagc tgcagctcgc gatgacccgg gtcgacatcg tcgcggcgat ccgcgcggcg
     2341 ctcgagtcga tccggccggc cgcgctggcg cgccggatcc agatctcgga cagcctgccg
     2401 ccggatctcg gggacgtgct cggcgactcc gatcggctcc agcaggtggt gtggaacctg
     2461 ctgtccaacg cggtcaagtt caccccgatg gacggcgtga tcgaggtccg cgccgagcgc
     2521 tcggggtcga ccctgcgcgt gatcgtccgc gacaccggca agggcatcgc ccccgagcac
     2581 ctgacgacga tcttcgagcg gttccgccag gtcgacagct cgacgacccg cgagcaccgc
     2641 gggctgggcc tgggcttggc gatcgtgcgc tatctcgtcg aggcccacgg cgggaccgtg
     2701 cacgccgaga gccccggccc cggccagggt gcgacgttca tcgtgaccct gccggtgcgc
     2761 accgatgcgc tggccgccgc cgtccgcagc cacgatagcc ggcgcccgct cgacgccgag
     2821 cggctgcgcg ggatccggat cctgctggtc gaggacgacg acgaggcccg cgagctgctc
     2881 gccgacgtgc tcgccgagac cggagcgacg gtcgcacagg ccgcttcggc ggcggacggc
     2941 atggcctgcc tgatcgcgga gcccccgcac gtgctgatca gcgacatcgg gatgccgttc
     3001 gaggacggca actcgttcct gcgccgcgtc cgcgcgctgc cgcccgagcg cggcggcgac
     3061 gtcccggcga tcgcgctcac cgcctacgcg cggccggagg accggcgcgc caccgaggag
     3121 gccgggttcc agctccacgt cgtcaagccg gtgcgtcccg accagctgct cgccgcgatc
     3181 gagtcgctcg tgcagatccg gcactgaccg ccggcgggcg ggctcacggc gcgtatgaca
     3241 gctgaaggag cgtgccgttg gcggacgtga ccgtgttgtt gaacgggttg gacgggatat
     3301 tcatcgggcc cgcccagccg cagtcgccgc cgccgttgat cgtggcgtgc aggttgtcag
     3361 gtgatacctg gacgttcccg cccgcgtggt tgccggcctt gatgaacatg ttcccactgc
     3421 tgagcgtgaa cgatgtcccg gtgagatcga tctgggcgac gccggccgcg ctgttgttgc
     3481 cgatgcagtc catcgcgacg ccgtacggca tgctcgtgac catggtgccg ctgccggaat
     3541 ggtcgagcat gccggtcgac gtcgcaaagc tgcgatcgct gctgtcgatc ttcatcgtcg
     3601 cgggatcgaa gcgcaccttg gtgaacgtcg tcttcacgtc cgtgccgggg ctgtttcccc
     3661 cgtgggtgta ctgcgcgaag ttcgccccgg tgagcgacag gtactcgcgg gggttattcg
     3721 ccatgccggc gcagtgcgcc tgccacggct tgcgggcatc gcccccgagg tacagcgtgt
     3781 aggtcccatc ggcggcgccc gacacgtgag acttgatgtc gccgcagctc gtcggcgtgt
     3841 agcacgtgaa gttgccgggc gtgttcatgc aggccgcggg gcatccgccg ttgctggtgg
     3901 cgcactcgtt gatgtcggtg cagctcaggc catcgcccat gaaccccgac ttgcagatgc
     3961 acttcgccgt gttcgtcatc gcgcacgtgg cgttcggatc gcaggtcagg ttggcgcagg
     4021 tgaccgcagg cggcgtgtcg atcggggcgt ccggtggcga ggccgcgtcg cccggcagcc
     4081 cgggctgcgc gttgaagccg cacgcggtca gccagagcgc cgtgaatgcc agacgtccac
     4141 ccatgggtcg gagcatatca acgatgaggc agaatctggc cggattcgga tctcggactg
     4201 tgaggtcgcg cacctgcgct acagctcgag atggaaccgg agcgagccaa cgaggatcgg
     4261 gccgcggtcg cggttgtagc cggggcgcgc gatcagctgg aggtcggccg cgacgaacgt
     4321 gccgcggtgg aggcgcgcgg tgtagtaggt ctcgacgatg gtctcgcggc cgtagcgcag
     4381 ccggccgtcg ccgagcagga agcccttgcc gccgagcgcg aggtagcggc gatggtcgtc
     4441 ggacaggccg ttggacaccg cggcgaggcc
//
LOCUS       DASVOR010001898         2478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340852,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001898 DASVOR010000000
VERSION     DASVOR010001898.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2478
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340852"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..672
                     /locus_tag="VF516_35225"
     CDS             <1..672
                     /locus_tag="VF516_35225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113053.1"
                     /translation="PPRVLTTRGAGRVDGEIYPIALADPQSGLAGLGLAASYDKTFGL
                     AIKVPNQTVSAPINQSHYAIGARYRLGLGESSSVAFGLDYARRQYVADRSGLGAVVLD
                     MPDVSYQAVSPGAWLRVPVAPSVTVFGGADGLLIFEAGGIQNRTSYGPATVYGLEATA
                     GVDIALARQIGVRIGLEVSQIMFKFTPQGPTLANNRDGDPSTQDVNGAADRSIGGTVM
                     ICLTY"
     gene            complement(703..1128)
                     /locus_tag="VF516_35230"
     CDS             complement(703..1128)
                     /locus_tag="VF516_35230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEX8113054.1"
                     /translation="MVATAKQAAQGEITTLLGRGAAFEGKLTFDGTVRIDGRFKGEVF
                     SDDVLVIGEGAHVEAEIDIGEVIVQGTVVGNIKAKRSIEIHAPGRVKGDLHTPSLQID
                     KGVIFEGRSFMENAVTTQPRPPVPPAVTPPKPGPTPVKP"
     gene            1298..>2478
                     /locus_tag="VF516_35235"
     CDS             1298..>2478
                     /locus_tag="VF516_35235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113055.1"
                     /translation="MQRSSEPRPVSGRHGGQRDPRVGTWLDGRYLVEERIATGGFGAV
                     YRARTPAGAPLALKVLHPSLTGDPNAVARFEREGATMTRLHDPHTVTTLAVGKAGDGT
                     RYIAMELLTGESLHARLARERALPWQTAAEIARAVCSSLAEAHALGVVHRDLKPDNIC
                     VEQRGGRELVKVIDFGIVKLAPGSAIDDGRELTCAGHMIGTYDYMSPEQIVGGACDAA
                     SDLYSLGVVLYEMLTGQRPFARLATPAAMMTALLTQTPQPPSVLAAIPPALDRIVMRC
                     LEREPTQRFSGATELAAALERLLERRLTAREEITAVHPMCPGALRAVPSCATWSDGWL
                     TRSSAGEDAAICRDPGATLVDDELRTGLVECWPAPAAANAIAPRWTDPRGMPAVVPPR
                     ST"
BASE COUNT          352 a          900 c          865 g          361 t
ORIGIN      
        1 ccgccccgcg tgctgaccac ccgcggcgcc ggccgggtcg acggcgagat ctatccgatc
       61 gcgctggccg atccgcagtc cgggctcgcc gggctcgggc tcgcggcgtc ctacgacaag
      121 acgttcggcc tggcgatcaa ggtcccgaac cagacggtca gcgcgccgat caaccagtcg
      181 cactacgcga tcggcgcgcg ctaccggctc ggcctgggcg agtcgtcgag cgtcgcgttc
      241 gggctcgact acgcgcgccg ccagtacgtc gccgatcgct cggggctggg cgcggtggtg
      301 ctcgacatgc ccgacgtcag ctaccaggcg gtgtcgccgg gcgcgtggct gcgcgtgccg
      361 gtcgcgccgt cggtcaccgt gttcggcggc gccgatggcc tcttgatctt cgaggccggg
      421 ggaatccaga accgcacgag ctacggcccg gccacggtgt acggcctcga ggccaccgcc
      481 ggcgtcgaca tcgcgctcgc caggcagatc ggggtccgga tcgggctcga ggtcagccag
      541 atcatgttca agttcacgcc ccaggggccg acgctggcga acaaccgcga cggcgatcca
      601 tcgacccagg atgtcaacgg cgcggccgac cgttcgatcg gcggcaccgt gatgatctgc
      661 ctgacgtatt gacgatcttc gcggcggcga tccgagattc tctcacggct tgaccggcgt
      721 cggccccggc ttgggcgggg tgacggccgg aggcaccggc gggcgcggct gcgtggtgac
      781 cgcgttctcc atgaacgagc ggccctcgaa gatcacgccc ttgtcgatct gcaggctcgg
      841 cgtgtgcaga tcgcctttca cgcggccggg cgcgtggatc tcgatcgagc gcttggcctt
      901 gatgttgccg acgacggtgc cctgcacgat gacctcgccg atgtcgatct cggcctcgac
      961 gtgagcgccc tcgccgatca ccagcacatc gtcggagaac acctcgccct tgaaccggcc
     1021 gtcgatgcgg accgtgccgt cgaacgtcag cttgccttcg aacgcggctc cgcggccgag
     1081 cagcgtcgtg atctctccct gagcagcttg ctttgcggtg gcaaccatgg cgacggacac
     1141 taacagatcc tctgcgcgcg tgccgggcag cggggcggtg gtcgtcgggg tggcggcgaa
     1201 cacctccacc tcgcgatctg ttcggatcaa ctgccaaaac ccacccaaca ttctttgcat
     1261 gaatacatcc gcgaggttcg ctcgttggta acagcgaatg cagcgctcct cggaaccacg
     1321 acctgtcagc ggacgtcacg gcggtcagcg tgatccacgg gtcggcacct ggctcgatgg
     1381 ccgctacctg gtcgaggagc ggatcgccac cggcgggttc ggcgcggtgt accgcgcgcg
     1441 caccccggcc ggcgcgccgc tcgcgctcaa ggtcctccac cccagcctga ccggcgatcc
     1501 gaacgcggtg gcgcggttcg agcgcgaggg cgcgacgatg acccggctgc acgacccgca
     1561 caccgtgacc acgctggcgg tcggcaaggc tggcgacggc acgcgctaca tcgcgatgga
     1621 gctgctcacc ggcgagagcc tgcacgcgcg gctcgcacgc gagcgcgcgc tgccatggca
     1681 gaccgcggcc gagatcgcgc gcgcggtgtg cagctcgctc gccgaggccc acgcgctcgg
     1741 cgtcgtccac cgcgacctca agccggacaa catctgcgtc gaacagcgcg gcggccgcga
     1801 gctcgtcaag gtgatcgact tcgggatcgt caagctcgcc ccgggcagcg cgatcgacga
     1861 cggcagggag ctgacctgcg cgggccacat gatcgggacg tacgactaca tgtcgcccga
     1921 gcagatcgtc ggcggcgcgt gcgacgccgc gtccgacctg tactcgctcg gggtcgtgct
     1981 ctacgagatg ctgaccgggc agcggccgtt cgcccggctc gccacaccgg cggcgatgat
     2041 gaccgcgctc ttgacccaga ccccgcaacc accgtcggtg ctcgccgcga tcccgccggc
     2101 gctggaccgc atcgtgatgc gctgcctgga gcgcgagccc acgcagcggt tctccggcgc
     2161 gaccgagctc gccgccgccc tcgagcgcct gctcgagcga cggctgaccg cgcgcgagga
     2221 gatcaccgcg gtccacccga tgtgcccggg cgcgctgcgc gccgtgccgt cgtgcgcgac
     2281 gtggagcgac ggctggctca cgcggtcgtc cgccggtgag gacgccgcga tctgccgcga
     2341 ccccggcgcg acgctggtcg acgatgagct ccgcaccggc ctggtcgagt gctggccggc
     2401 gcccgccgcg gcaaacgcga tcgcgccgcg ctggaccgac ccgcggggca tgcccgcggt
     2461 ggtgccgccg cgatcgac
//
LOCUS       DASVOR010001899         2229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_312465,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001899 DASVOR010000000
VERSION     DASVOR010001899.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312465"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2229)
                     /locus_tag="VF516_35240"
     CDS             complement(<1..>2229)
                     /locus_tag="VF516_35240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8113056.1"
                     /translation="TWSAAPLGSPDAVEAEVRAQLAAGGLPAGLCRVVVATVSLEGGP
                     IQCVTYRGADGGYAEDARYRGVHPMIGRRMHLWRLAGFELERLPSVEDVYLYRGIART
                     NPRDERLFAIGEVRDLTPVRDAAGRIVQLPYLERVLGEALAALRRVQAARPARDRLQW
                     NRVILGFWPPLTLSDAELGDVIRRLAPETEGLGIEQVVVHARIPALGAGAAGELRDAV
                     LRISNTLDGGLHLRVTEPPDYPIATLTEYEQKVIALRRRGLIYPYEIVRLLAPRDAGA
                     ADGFPRGEFTEHDLDAAGALVPVRREPGCNTANIVAGVLRSFTPEVPEGVTRVVLLGD
                     AHKEMGSLAEPECRRILAAIDLAERMAVPLEWFALSAGAKISRQSGTENMDWIAAVLR
                     RIIEFTQRGGEINIVVCGINVGGQPYWNAEATMLMHTRGILVMIDDASMVLTGKQALD
                     YSGSTSAEDNQGIGGYERIMGVNGQAQYWAADLGHACRILLAHYAHTYVVPGERFPRR
                     AATADPRARDVRSASHPGELATIGDVFGPRNADRKRPFDIRAVMRAITDLDHEPLERW
                     RDQRDAEIAVVWDAHLGGTPLCMIGIESHALPRRGFVPADGPVQWTSGTLFPRASKKI
                     ARALHAASGNRPVVVVANLAGFDGSPESMRELQLEYGAEIGRAVVNFDGPIVFCVVSR
                     YHGGAFVVFSKRLNDQLEVIAVEGARASVIGGAPAAAVVFARDVDTRTARDPRIAALE
                     DAL"
BASE COUNT          295 a          788 c          859 g          287 t
ORIGIN      
        1 agcgcgtcct cgagcgcggc gatccggggg tcccgggcgg tccgggtgtc gacgtcgcgg
       61 gcgaacacga ccgccgccgc cggcgcgccg ccgatcaccg aggcgcgggc gccctcgacg
      121 gcgatcacct cgagctggtc gttgagccgc ttggagaaca cgacgaacgc gccgccgtgg
      181 tagcgcgaca cgacgcagaa cacgatcgga ccgtcgaagt tgaccaccgc gcggccgatc
      241 tcggcgccgt actcgagctg gagctcgcgc atcgactcgg gcgagccgtc gaacccggcg
      301 aggttggcga cgaccacgac cgggcggttg ccgctcgccg cgtggagcgc acgcgcgatc
      361 ttcttggacg cgcgcgggaa cagcgtgccg gatgtccact ggaccggacc gtcggcgggg
      421 acgaagccgc ggcgcggcag cgcgtgcgac tcgatgccga tcatgcacag cggggtgccg
      481 ccgaggtggg cgtcccacac cacggcgatc tcggcgtcgc gctggtcgcg ccagcgctcg
      541 agcggctcgt ggtcgaggtc ggtgatcgcg cgcatcacgg cgcggatgtc gaacggccgc
      601 ttgcgatcgg cgttgcgcgg gccgaacacg tcgccgatcg tcgcgagctc gcccgggtgg
      661 gacgcgctgc gcacgtcgcg cgcgcggggg tcggcggtcg cggcgcggcg cgggaaccgc
      721 tcgccgggca cgacgtaggt gtgcgcgtag tgggccagga ggatccggca ggcgtggccg
      781 aggtcggccg cccagtactg ggcctggccg ttgacgccca tgatgcgctc gtagccgccg
      841 atgccctggt tgtcctcggc cgaggtgctg cccgagtagt cgagcgcctg cttgccggtc
      901 agcaccatcg acgcgtcgtc gatcatcacg aggatgccgc gggtgtgcat cagcatcgtc
      961 gcctcggcgt tccagtacgg ctggccgccg acgttgatcc cgcacaccac gatgttgatc
     1021 tcgccgccgc gctgggtgaa ctcgatgatc cggcgcagca ccgcggcgat ccagtccatg
     1081 ttctcggtgc cgctctgccg cgagatcttg gcgcccgccg acagcgcgaa ccactcgagc
     1141 gggacggcca tccgctcggc gagatcgatc gccgcgagga tccggcggca ctcgggctcg
     1201 gcgagcgagc ccatctcctt gtgggcgtcg ccgagcagca cgacgcgggt gacgccctcg
     1261 gggacctcgg gggtgaagct gcgcaggacg ccggcgacga tgttcgcggt gttgcagccg
     1321 ggctcgcggc ggaccgggac cagcgcgccg gcggcgtcga ggtcgtgctc ggtgaactcg
     1381 ccgcgcggaa agccatcggc ggcgccggcg tcgcgcgggg cgagcagccg gacgatctcg
     1441 tagggataga tcaggccgcg gcggcgcagc gcgatcacct tctgctcgta ctcggtcagc
     1501 gtcgcgatcg ggtagtccgg cggctcggtg acccgcaggt gcagcccgcc gtcgagcgtg
     1561 ttggagatcc gcagcacggc gtcgcgcagc tcgcccgcgg cgccggcgcc gagggcagga
     1621 atccgggcgt gcacgacgac ctgctcgatg cccaggccct cggtctcggg ggcgagccgg
     1681 cggatcacgt cgccgagctc ggcgtcgctc agcgtgagcg gcggccagaa gcccaggatc
     1741 acgcggttcc actgcagccg atcgcgcgcc ggccgcgcgg cctggacgcg gcgcagcgcg
     1801 gcgagcgcct cgccgagcac gcgctcgagg tacggcagct ggacgatccg gccggccgcg
     1861 tcgcgcaccg gggtcagatc gcggacctcg ccgatcgcga acagccgctc gtccctgggg
     1921 ttggtccggg cgatgccgcg gtagaggtag acgtcctcga ccgacggcag gcgctcgagc
     1981 tcgaacccgg cgagccgcca caggtgcatg cggcggccga tcatcgggtg gacgccgcgg
     2041 tagcgggcat cctcggcgta gccgccgtcg gcgccgcggt aggtgacgca ctggatcggt
     2101 ccgccctcga gcgacacggt cgccaccacg acgcggcata gccccgccgg caggccgccg
     2161 gcggcgagct gcgcccggac ctcggcctcg acggcgtcgg gcgacccgag cggcgcggcg
     2221 ctccaggtg
//
LOCUS       DASVOR010001900         4949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_546142,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001900 DASVOR010000000
VERSION     DASVOR010001900.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4949
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546142"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="VF516_35245"
     CDS             <1..701
                     /locus_tag="VF516_35245"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8113057.1"
                     /translation="RSDGASYVLAGARHGLFTEHLIAGLRGAAGSGDGVVRVLDLYHH
                     VQRNVVARFPAQRPVLKAEMEDNYVVALCPSAARPALTAAAAPSEFVYDVLLVYAPDD
                     RDRAWARVFTQLLEERGVRVCTVDDDAELGRDRLLELERLVSTSRFTLPVLTPRFSAG
                     RFQELQTVLAQQLGIEDGIARLIPVVREPCEARLGLRSLVHLEMIRDETVVPGIERLV
                     RTLRRQPAPRPGPR"
     gene            737..1561
                     /locus_tag="VF516_35250"
     CDS             737..1561
                     /locus_tag="VF516_35250"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX8113058.1"
                     /translation="MFGRRRDGVLAKVPPYRQIMPFLMRGRNESSVLFEQHLDMSRAQ
                     PWLEDWHRRTGQHATAFHLVLHAIARVLHDRPHLNRFIVGRRIYDRRGVFMTFAAKKA
                     MRDGAPLATVKREFRRGESFAEMVAALNADIQVAKSDAISKVDKELSVFLRFPNFLLA
                     ASVALLRWLDGHGLAPRALIGDDPMYTSAFIANLGSLKIDAAYHHLYEYGNCPLFCTV
                     GRLTTAPIVVDGTVQVRPSLLLRYSYDERVEDGFYAASAIALVRDWIEDPASWIAG"
     gene            1624..2415
                     /locus_tag="VF516_35255"
     CDS             1624..2415
                     /locus_tag="VF516_35255"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8113059.1"
                     /translation="MTHPFPDLETSEATYEGKTHTVFRGGRGPAVIVIHEIPGLHPGV
                     VAFGRRLIAAGMTAYFPSLFGEPGREVSTGYMLREISRACVSSEFATWATRRTSPIVS
                     WLRALARDAHAACGGPGVGAVGMCLTGGFALAMMVDDTMVAPVLSQPSLPFPISASRR
                     RDVGVSDDDLVKIRARCAAGTPVMGLRFTGDRAVPGDRFARLREELGDRFIGIEIDSS
                     AGNPYGLPANAHSVLTHHLVDEPGHPTRVALERVIAFFRDRLGVA"
     gene            complement(2452..3507)
                     /locus_tag="VF516_35260"
     CDS             complement(2452..3507)
                     /locus_tag="VF516_35260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113060.1"
                     /translation="MKLALVVVMMVSGLAVAAPKRDARPATAAQRAEYKKHMKAGWAL
                     QKQQKWAEAVVEFEAALAAVEADQRAFSELGFSAMNAGDFKKARVADEQAVQVASDKK
                     VKAAALYNLGLVFEKTSDRSGALRAYLASLELRPNKVVEQAVGRLGATPSAPPPFCAA
                     GAKPCDCVLQDAFSGGPGPDEGAACEVKKDAPVPGFHVVRVSRPFHSESYEYLLDEHA
                     QLAAVIAGDYEYHWGRYSFHQAADRIENRTVGGHRVLWIEVSDELDETSGDETTMAID
                     TTRNTLVTLCLPGDGKAPTRCPLRSVPILREQDGATKSKTVLDLAIASDGTATVKLKQ
                     GPSDEAINARVGPHKLW"
     gene            4767..>4949
                     /locus_tag="VF516_35265"
     CDS             4767..>4949
                     /locus_tag="VF516_35265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113061.1"
                     /translation="MSTGNSTRRHRSEPEQLVLWKKRGGKRRGAGRPPKGMRAGSSHQ
                     QRPYLHARYPVHVTLRV"
BASE COUNT          708 a         1819 c         1642 g          780 t
ORIGIN      
        1 cccgcagcga cggcgcgtcc tacgtgctcg ccggggcgcg ccacggcctg ttcaccgagc
       61 acctgatcgc ggggctgcgc ggcgcggcgg gcagcggcga cggcgtcgtc cgcgtgctcg
      121 acctctacca ccacgtgcag cgcaacgtgg tcgcccggtt cccggcgcag cgcccggtgc
      181 tgaaggccga gatggaggac aactacgtcg tcgcgctgtg cccgagcgcg gcgcgccccg
      241 cgctgaccgc cgcggcggcg ccgagcgagt tcgtctacga cgtgctgctg gtgtacgcgc
      301 ccgacgaccg cgaccgcgcc tgggcccgcg tgttcaccca gctgctcgag gagcgcggcg
      361 tccgggtgtg cacggtggat gacgacgccg agctcggccg cgatcgcctg ctcgagctcg
      421 agcgcctggt gtcgaccagc cggttcacgc tgccggtgct gaccccgcgg ttctccgccg
      481 gccggttcca ggagctgcag accgtgctgg cccagcagct cgggatcgag gacggcatcg
      541 cgcggctgat cccggtcgtc cgcgagccct gcgaggcccg gctcggcctg cgctcgctcg
      601 tccacctcga gatgatccgc gacgagaccg tggtgcccgg catcgagcgc ctggtccgca
      661 ccctgcgccg ccagccggcg ccccggcccg ggccgcgctg acccccgcac cgcccgcgcg
      721 cgcgtaggat acggccatgt tcggacgtcg ccgcgacggt gtgctcgcca aggtgccccc
      781 gtatcgccag atcatgccgt tcctcatgcg aggcaggaac gagtcgagcg tgctgttcga
      841 gcagcacctc gacatgtcgc gcgcgcagcc ctggctcgag gactggcacc ggcggaccgg
      901 acagcacgcg accgcgttcc acctcgtgct gcatgcgatc gcccgcgtgc tgcacgaccg
      961 gccgcacctc aaccgcttca tcgtcggccg gcggatctac gaccggcgcg gcgtgttcat
     1021 gacgttcgcc gccaagaagg cgatgcgcga cggcgcgccg ctggcgacgg tcaagcgcga
     1081 gttccggcgc ggcgagagct tcgccgagat ggtcgccgcg ctcaacgccg acatccaggt
     1141 cgccaagagc gacgcgatca gcaaggtcga caaggagctg tcggtgttcc tgcggtttcc
     1201 gaacttcctg ctcgccgcct cggtcgcgct gctgcgctgg ctcgacggcc acggcctggc
     1261 gccgcgcgcg ctgatcggcg acgacccgat gtacaccagc gcgttcatcg ccaacctggg
     1321 cagcctcaag atcgacgccg cgtatcacca cctctacgag tacggcaact gcccgctgtt
     1381 ctgcaccgtc ggccggctca ccaccgcccc gatcgtggtc gacggcaccg tccaggtccg
     1441 cccgtcgctg ctgctccgct acagctacga cgagcgggtc gaggacgggt tctacgccgc
     1501 atcggcgatc gcgctggtcc gcgactggat cgaggatccg gccagctgga tcgccgggtg
     1561 atccggatgt ctcgacctcg cctgggccgc gcgcccggcg tccggagctg ctagcgtcgc
     1621 ggcgtgaccc acccatttcc ggatctggag accagcgagg cgacgtacga gggcaagacc
     1681 cacacggtgt tccgcggcgg ccgcggcccg gcggtgatcg tgatccacga gatcccgggg
     1741 ctgcatcccg gggtcgtcgc gttcggccgg cggctgatcg ccgcgggaat gaccgcgtac
     1801 ttcccgtcgc tgttcggcga gcccgggcgc gaggtcagca cggggtacat gctccgcgag
     1861 atctcgcgcg cgtgcgtgtc gagcgagttc gcgacctggg cgacccgcag gaccagcccg
     1921 atcgtgagct ggctgcgggc gctcgcccgc gatgcccacg ccgcgtgcgg cggtccgggc
     1981 gtcggcgcgg tcggcatgtg cctgaccggc ggcttcgcgc tcgcgatgat ggtcgatgac
     2041 accatggtcg ccccggtgct gagccagccc tcgctgccgt tcccgatctc cgcctcgcgc
     2101 cgccgtgacg tcggagtgtc cgacgacgac ctggtcaaga tccgggcgcg ctgcgccgcc
     2161 ggcacgccgg tgatggggct gcggttcacc ggcgatcgcg cggtccccgg cgatcgcttc
     2221 gcgcggctgc gcgaggagct cggtgaccgc ttcatcggca tcgagatcga ctccagcgcc
     2281 ggcaacccgt acggcctgcc cgccaacgcg cactcggtgc tgacccacca cctggtcgac
     2341 gagcccggcc atccgacgcg ggtcgcgctc gagcgcgtga tcgcgttctt ccgcgaccgg
     2401 ctcggcgtcg cgtgaccggc cgcgaccgct ccggccaccg gcgtctgccg gctaccagag
     2461 cttgtgcggg ccgacccgcg cgttgatcgc ctcgtcgctc gggccctgct tcagcttgac
     2521 cgtcgccgtc ccgtcgctgg cgatcgccag atccagcacc gtcttcgact tcgtcgcgcc
     2581 gtcctgctcg cgcaggatcg gcacgctgcg cagcgggcag cgcgtcggcg ccttgccatc
     2641 tccgggcagg cacagcgtga ccagcgtgtt ccgcgtcgtg tcgatcgcca tcgtggtctc
     2701 gtcgcccgag gtctcgtcga gctcgtcgct gacctcgatc cacagcacgc ggtgcccgcc
     2761 gaccgtccgg ttctcgatcc tgtcggcggc ctggtgaaac gagtaccggc cccagtggta
     2821 ctcgtagtcg ccggcgatca ccgcagcgag ctgggcgtgc tcgtcgagca ggtactcgta
     2881 cgactcgctg tggaacggcc gcgacacgcg cacgacgtgg aagcccggca ccggcgcatc
     2941 cttcttgacc tcgcacgccg cgccctcgtc gggcccgggt ccaccgctga acgcatcctg
     3001 cagcacgcaa tcgcacggct tcgcgcccgc ggcgcagaac ggcggcgggg cgctcggcgt
     3061 ggcgccgagc cggccgaccg cctgctcgac caccttgttc gggcgcagct cgagcgacgc
     3121 gaggtaggcg cgcagcgcgc cggaccggtc gctggtcttc tcgaacacca gcccgaggtt
     3181 gtagagcgcc gccgccttga ccttcttgtc ggacgccacc tgcaccgcct gctcgtcggc
     3241 gacgcgcgcc ttcttgaagt cgccggcgtt catcgccgag aacccgagct cggagaacgc
     3301 gcgctggtcg gcctcgaccg cggcgagcgc ggcctcgaac tcgaccaccg cctcggccca
     3361 cttctgctgc ttctgcagcg cccagcccgc cttcatgtgc ttcttgtact cggcgcgctg
     3421 cgccgcggtg gcaggcctgg cgtcgcgctt cggcgcggcg accgcgagcc ccgagaccat
     3481 catgaccacg accagcgcca gcttcatggc cgcacggtgt aacgcgttcc cgcggcgaga
     3541 tccatgcccg ctacgcagcc cgcgcgcgca ggtacgcgag gctctgcgga aggtcacgga
     3601 tcagccggag caggatgtcc atcgcctcgg tctggacgtt cctgccagac tcccaggaga
     3661 gatcgtgttg gcgccgagac gaaggaggcg cgtcaggtct gcctgcgtca gctcgaagcg
     3721 cgtccggatc gctcggatct cgtccgccga gagcaagcca tgcctcttgc ggtagatggc
     3781 gatcgcatcc tctccgagcc gcttggcgtt ctgaaaccgg agcaggatct cgccgcacct
     3841 ggggcagcgc agatgcgtgg cggacggcac cgcgatgtcc tcgccgttga ccgggaggcg
     3901 cagtgtggcc cgcctctcca gcatcgtcgt gccgcagctc gggcatgcgt cgctgggcag
     3961 cacgcgtcca cgccgaggag ccgtcttgcg gtgatgcgtc ttcttcatga ccaccggcat
     4021 cctcgtggaa ggagaccacg atgcagtcca ggcgcaggat cagcttgacg tacaggaccg
     4081 ccccggcaag ctcgggtttg aagacgtaca gccacttccc ccgtccaggc aagcgggcgc
     4141 tcgtggcgca agctcggctc ggcagccaag agtcaacttg ctggaatgac ctcctgcatg
     4201 aacggtagat gctgtcgtcc caaccgacca tatgaggcca gcattcgcgc gcgtcggcgt
     4261 ccgaagctgc tagcgtcgcg gcatcgagct cgactccagc tccggcaacc cgtacagccg
     4321 gcccgccgac gcgcactccg tgttgaccaa cacctggtcg accagcccag cctccgacac
     4381 ggtcgtgctg gagcaggtga tcgcgttcgt ccacgagcgg ctcgccgtca cgtaacgatt
     4441 gcatgttcgc ggtccgcgcc gccctcctga ccaggcgaat ctaatcagta aagttgctcg
     4501 atgcacccgc cgagcctccc cttgcccggt ctccgagagc cacgagcatc acacgctctg
     4561 gatcccattc actgtcgagt gcgctgcata ttccctgcgg cagcggagat ctgtgcaaga
     4621 ccacgcgcga acgcgatatc gacccgcgtt catcttgaac ggcgcgataa tcgcgcattc
     4681 cagtatccac aattagcaca atattccgcc gataaatatc tgccgaatca attgcgctgg
     4741 ttcgaatcag gcactatata gttcgcatgt ccaccggaaa ctcaacgcgc aggcaccgca
     4801 gcgagcccga gcagctggtc ctgtggaaga agcgcggtgg caagcggcgc ggcgcaggtc
     4861 gcccaccgaa gggcatgcgg gccggttcgt cgcaccaaca gcggccgtac ctccatgccc
     4921 ggtatcccgt ccacgtcaca ttgcgggtc
//
LOCUS       DASVOR010001901         2987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_340881,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001901 DASVOR010000000
VERSION     DASVOR010001901.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340881"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VF516_35270"
     CDS             <1..182
                     /locus_tag="VF516_35270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113062.1"
                     /translation="WRRALVGAPDPATAAQLLRELPLSASQILRVAERALASGMTAPD
                     ALRAEALRTARDAGG"
     gene            complement(183..713)
                     /locus_tag="VF516_35275"
     CDS             complement(183..713)
                     /locus_tag="VF516_35275"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8113063.1"
                     /translation="MFLLCQISPSMQQWFAAWPRLVDARGYVGLISHILGHGSWEHVI
                     GNFMLILLIGPILEERHGSVRLLILIVLTAVLTGLLHAALSDGLMIGSSGIAFMMILL
                     ASMVNVRSGEIPLTFLAVAAIHTNGELVRLFRSDGIAHSAHLIGGAAGAIFGFLGAQP
                     RLALPTTPAKRQLATR"
     gene            967..2865
                     /locus_tag="VF516_35280"
     CDS             967..2865
                     /locus_tag="VF516_35280"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8113064.1"
                     /translation="MSVRRDIEPSIRDTSQSSGRNVIAVIGIDRYAHWPRLGNAVNDA
                     RGVAALFGQLGFEQITEPLLDERATGKATRELVNDELRGLGPNDSLVVFYAGHGSTRT
                     DKLGRQMVKTGYLIPADASDSSASWIELSGWLRALSVLPPRHILVILDACHSGIALGP
                     IIKWRDSGTWKDTPAATLRSRRSRRIITSALDDQTALDSGPLHGHSLFTGCVIEALTH
                     GIAHAGQRETTGSELALYVQHRVMTHPSSEQTPDFGTFDFDERGELMIPLRTTGSHPD
                     GIAEIAAGASPPAHSPPAHSTPAPRDPPSSRESAARPRWPRWLRGSVADKATRLLAVL
                     ALVVPSLILVVIDPAGGGAPPASESISPSQAPSSRDDRRRSNTESARSALPESIHVDV
                     ARATLVALLKERMNAGCRVLAAPQEIRGDKTNHITSRGDHCVAEIFVSATAAERLQLT
                     LTDPLGEALPVPPPSPTVELTHCPQLVGEYTVVSHPATATPYVMAALDCDGTTMAAHG
                     PNTAAAEAARATLAARVQSRLAAGCVMSGAPRPAMGKTSWTTTVPESARCLEHLAESS
                     ALDNPVVITVTDPAGQQLSGGPMASLTVRYCPTRTGEYTYQARPKNDAPYAVATLECP
                     RNAREPRR"
BASE COUNT          496 a         1094 c          991 g          406 t
ORIGIN      
        1 tctggcgccg cgcgctcgtc ggcgcgcccg accccgcgac cgccgcgcag ctgctgcgcg
       61 agctgccgct gtcggcgtcg cagatcctgc gagtcgccga gcgcgcgctg gccagcggga
      121 tgaccgcccc cgacgcgctg cgcgccgagg ccctgcgcac ggcccgcgac gccggcgggt
      181 agctacctgg tcgcgagctg ccgtttcgcc ggtgtcgtgg gcagtgccag cctcggctgt
      241 gcgccgagga acccaaaaat cgcgccagcc gctccgccga tgaggtgcgc agaatgcgcg
      301 atgccatcgg accggaacag gcgcaccagc tcgccgttgg tgtggatcgc ggcgactgcc
      361 aggaacgtga gcgggatctc tccactgcgc acgttcacca tggaagcgag caagatcatc
      421 atgaacgcga tgccgctcga gccgatcatc aggccgtcgg acagcgcggc atgcaagagc
      481 ccggtcagga ccgcggtgag cacgatcaag atcagtagcc gcaccgagcc gtggcgctct
      541 tccaggatcg gcccgatcag caagatcagc atgaagttgc caatcacgtg ctcccagctt
      601 ccgtggccga ggatatggct gatcaggccg acgtagccgc gggcatcgac caggcgcggc
      661 cacgccgcga accattgctg catcgacggc gagatctgac agagcagaaa cactccgacc
      721 gctgcaatcg tgaacgtcag gacaaccggt gcgttgtacg tgatcctcaa cctcaccaca
      781 ctcgaacact agcacgcagg gcgtgcatcg cacctccgga gctcctcgcg atcctgtcga
      841 tccacgcgtg atgtccgtcg attatcggcg aatcacggca gcatcgcaca gctgactacc
      901 cgcgcacgca ctccgcgtgt cgtggcgagg ccacttgcgt cgcctatcca cgagagtcga
      961 caacccatga gtgtgcggcg cgacatcgag ccttcaatcc gcgatacgag ccagtcgtca
     1021 ggccgcaacg tgatcgcggt gatcgggatc gatcgctacg cccactggcc ccgtctcggc
     1081 aacgccgtga acgatgcgcg gggcgtcgcc gcgctgttcg gacagctcgg gttcgagcag
     1141 atcaccgagc cgctcctcga tgagcgcgcg accggcaagg caacgcggga gctcgtgaac
     1201 gatgagctca gaggactggg cccgaatgac agcctcgtcg tgttctacgc gggccacggc
     1261 agtacgcgga ccgacaagct cggccgccag atggtcaaga ccgggtacct catcccggcg
     1321 gacgcgtcgg acagctcggc gtcgtggatc gagctcagcg gctggctgcg ggcactctcg
     1381 gtcctgccac caaggcacat cctcgtgatc ctcgacgcat gccacagcgg gatcgcgctg
     1441 ggaccgatca tcaagtggcg cgacagcggc acatggaagg acacgccggc cgcgacgctg
     1501 cgctcccggc ggagccggcg gatcatcacc tcggcgctcg acgatcagac cgcgctcgac
     1561 agcggcccgc tgcacggcca ctcgctgttc actggctgcg tgatcgaggc gctgacccac
     1621 ggcatcgcgc acgccggcca gcgcgagacc accggctcgg agctcgccct gtacgtccag
     1681 catcgcgtca tgacccatcc cagctcggag cagacgccgg atttcggcac gttcgacttc
     1741 gacgagcgcg gcgagctgat gattccactg cggaccaccg gatcgcaccc ggatggcatc
     1801 gcggagatcg cggcaggcgc cagcccgccg gcacactcgc cgccggcgca ctcgacgccg
     1861 gcacctcgcg acccgccttc gtcgcgcgag tccgctgcgc gtccgcgatg gccgcgatgg
     1921 ctgcgcggct cggtggccga caaggcgacc cggctgctcg ccgtcctcgc gctcgtggtg
     1981 ccgtcgctga tcctcgtggt catcgaccca gccggtggcg gcgcgccgcc ggcgtccgag
     2041 tcgatctcgc ccagccaggc gccgtcgtcg cgagacgatc gccggcggtc gaacacggag
     2101 tccgcgcgct ccgcgctgcc cgagagcatc catgtcgacg tggcccgagc aacgctcgtc
     2161 gcgctgctca aggagcgcat gaacgccggc tgtcgcgtgc tcgcggcgcc gcaagaaatc
     2221 cggggagaca agaccaacca catcacgtcc cgcggcgatc actgtgtcgc cgagatcttc
     2281 gtcagcgcca ccgccgcgga gcgcctgcag ctgacgctca ccgatccgct tggagaggcg
     2341 ctgccggtcc caccgcccag cccgactgtc gagctcacgc actgccccca gctggtgggg
     2401 gagtacacgg tcgtgtcgca cccggccacc gccacgccct acgtcatggc ggcgctggac
     2461 tgcgatggca cgacgatggc ggcgcacggc ccgaacaccg ccgcggccga ggccgcgcgc
     2521 gcgacgctcg ccgcgcgagt ccagagccgg ctcgccgccg gatgcgtgat gtccggtgcg
     2581 ccgcggccgg cgatgggcaa gacgagctgg accaccaccg ttcccgagtc cgcgcgatgc
     2641 ctcgagcacc tcgccgagag cagcgcactg gacaaccccg tggtcatcac ggtgaccgat
     2701 ccggcgggcc agcagctctc cggtgggccg atggcgtcgc tcacggttcg ctattgcccg
     2761 acgcgaaccg gcgagtacac gtaccaggcc cggccgaaga acgacgcgcc gtatgcggtg
     2821 gccacgctgg aatgcccgcg gaacgcccgc gaacccaggc gctgaccgcg cagccgcgcg
     2881 ctacccggtc gcgaatcgct cggcgtggcg cgccagcgcg gccgcgatga tcgccgtcga
     2941 gtagatgccg cggacatcgg cgtacagccg cccgccctga tcgccgg
//
LOCUS       DASVOR010001902         2977 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_108486,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001902 DASVOR010000000
VERSION     DASVOR010001902.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108486"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2977
                     /locus_tag="VF516_35285"
     CDS             <1..>2977
                     /locus_tag="VF516_35285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8113065.1"
                     /translation="GAATTAVHALWADGRGLVVGNGATVELSGGDGVWHRLGSDAGLG
                     RDSIEGVLRDREGALWIRTSAHLWYLPVGAARATDLRDGLPTGFDAVGAPNAMVIGPH
                     GDVLIGTDDGVTYRDGNRWRTIGRASGMPAATTRALLVDRDGDLWIGSAGLFQLRGRG
                     LIEHHNRASGLTGEIVWTYRRDRDGTLLAGTNRCLARAAGGRWTCVPGTEDRVVRAVV
                     LAPQGGMFIGGAPSDLLYIDRDGRTTSLGDPDRLDRFIFALALGPEGDLWIGTRSGLY
                     RLPGAVPGPIVPVAIPGIGPARFGSFAVVGDRLWTTADPGGVAVLDHGAGSARGTWRV
                     FDQTAGLRSASVSYLIARSDGRLCTAYTEALGATCFGYDGSRPVGITHIGAAEGLTSG
                     MVYFLGEDRERRLWIGTGDGVNVLGPGGPDHFDKSDGLAGNDSASQAFFLDRDGSVWL
                     GSTGGGSHVLAQHYTGAPTAPRTVLLDGRLGDLPIHGAPPEPLEVAHDHSALLIELAA
                     GTLLDPRRVEYQARLLPVETEWGAIHQRSAHYPGLLPGGYRFEVRARVDAGTWGPTTG
                     FAFAVLPAWWQTRWFLALIIGAGLGAVGGAFTWRQRTVLRRRTRQLHAEIDASFRAVI
                     DLMPDLITLQRDRRVIYLNRAIRRLLGIDGDERRAEADLIERVHPDDRAQVIELFRKV
                     GELELGVVSDLLEIQIRSADGSWRTCEVSGIRVELGGELTVVSTGRDVTERQRMRAKL
                     LMSDRMASLGTLAAGIAHEINNPLAYVIGNLEAMTEVLGGPERPASTGERAELGAQVR
                     DARDGAERVRKIVHGLRSFSRSDAETRAPLALPDVLEAAIRLTGNEVRHHAELVRELG
                     PVPLVVADDGRLTQVFINLLVNAAHAIPAGRSSDNRITVRTRTDDLGRAVIEIEDTGT
                     GIPAELQSRVFDPFFTTKGVGEGTGLGLSICHGIISGLGGQISIESAPTRGTVVRIVL
                     PPHVGDEVAEAAPAVVAEPA"
BASE COUNT          372 a         1115 c         1097 g          393 t
ORIGIN      
        1 ggggccgcga cgaccgcggt ccacgcgctg tgggccgacg gtcgcggcct ggtcgtgggc
       61 aacggggcga ccgtcgagct cagcggtggc gacggcgtgt ggcatcgcct cggcagcgac
      121 gccgggctgg gccgcgattc gatcgagggc gtgctgcgcg atcgcgaagg cgcgctgtgg
      181 atccggacat cggctcacct gtggtacctg ccggtgggcg cggcgcgcgc gaccgacctg
      241 cgcgacggcc tgccgaccgg gttcgacgcg gtcggcgcgc cgaacgcgat ggtgatcggc
      301 ccgcacggcg acgtgctgat cgggaccgac gacggggtca cctaccgcga cggcaatcgc
      361 tggcgcacga tcggccgcgc gtccggcatg ccggcggcga ccacgcgcgc gctgctggtc
      421 gatcgcgacg gcgatctgtg gatcggctcg gccgggctgt tccagctccg cggccgcggc
      481 ctgatcgagc accacaaccg ggcgagcggt ctgaccggcg agatcgtgtg gacctaccgg
      541 cgcgatcgcg acggcacgct gctcgccggc accaaccgct gcctggcgcg cgcggccggt
      601 ggacgctgga cgtgcgtgcc cggcaccgag gaccgcgtcg tccgcgccgt ggtgctcgcg
      661 ccccagggcg gcatgttcat cggcggcgcg ccgtcggatc tgctctacat cgatcgcgac
      721 ggccgcacga cctcgctcgg cgaccccgat cggctcgatc gcttcatctt cgcgctcgcg
      781 ctcggccccg agggcgatct gtggatcggg acgcgcagcg ggctgtaccg gctgcccggc
      841 gcggtgcccg gcccgatcgt gccggtcgcg atccccggca tcggcccggc gcggttcggc
      901 tcgttcgcgg tggtcggcga ccggctgtgg accaccgccg acccgggcgg cgtcgccgtg
      961 ctcgaccacg gggcggggag cgcgcgcggg acctggcgcg tgttcgacca gaccgccggg
     1021 ctgcgcagcg cgtcggtgag ctacctgatc gcgcgcagcg acggccggct gtgcacggcg
     1081 tacaccgagg cgctcggcgc gacctgcttc ggctacgacg gcagccgccc cgtcggcatc
     1141 acccacatcg gcgcggccga gggcctgacc tcggggatgg tgtacttcct cggtgaggat
     1201 cgcgagcgcc ggctgtggat cggcaccggc gacggcgtca acgtgctggg cccgggcggt
     1261 ccggaccact tcgacaagag cgacggcctg gccggcaacg actcggcatc gcaggcgttc
     1321 ttcctcgatc gcgacggctc ggtgtggctg ggctcgaccg gcggcggcag ccacgtgctc
     1381 gcccagcact acaccggcgc accgaccgcg ccgcgcaccg tgctgctcga cggccggctc
     1441 ggcgatctgc cgatccacgg cgcgccgccc gagccgctcg aggtggcgca cgaccacagc
     1501 gcgctgctga tcgagctcgc cgccggcacc ctgctcgatc cgcggcgcgt cgagtaccag
     1561 gcccgcctgc tgccggtcga gaccgagtgg ggtgcgatcc accagcgctc ggcccactac
     1621 ccgggtctgc tccccggcgg ctaccgcttc gaggtccgcg cccgggtcga cgccgggacc
     1681 tggggcccga ccaccgggtt cgcgttcgcc gtgctgccgg cgtggtggca gacccgctgg
     1741 ttcctcgcgc tgatcatcgg cgccgggctc ggcgcggtcg gcggcgcgtt cacctggcgc
     1801 cagcgaaccg tgctgcgccg ccgcacccgc cagctccacg ccgagatcga cgccagcttc
     1861 cgcgccgtga tcgacctgat gccggacctg atcaccctgc agcgcgatcg cagggtcatc
     1921 tatctcaacc gcgcgatccg ccgcctgctc ggcatcgacg gggacgagcg ccgggcggag
     1981 gccgacctga tcgagcgggt ccaccccgac gatcgcgccc aggtgatcga gctgttccgc
     2041 aaggtcggcg agctcgagct cggcgtggtg tcggacctgc tcgagatcca gatccgcagc
     2101 gccgacggca gctggcggac ctgcgaggtc tcggggatcc gggtcgagct cggcggcgag
     2161 ctcaccgtgg tgtcgaccgg ccgcgacgtc accgagcgcc agcggatgcg cgccaagctc
     2221 ctgatgtccg accgcatggc gagcctgggc acgctcgccg ccggtatcgc gcacgagatc
     2281 aacaacccgc tcgcctacgt gatcggcaac ctcgaggcga tgaccgaggt cctcggcggc
     2341 cccgagcgcc cggcgagcac cggcgagcgc gccgagctcg gcgcccaggt gcgcgacgcc
     2401 cgcgatggcg ccgagcgcgt gcgcaagatc gtccacggcc tgcgctcgtt cagccggtcc
     2461 gacgccgaga cccgcgcccc gctcgcgctg cccgacgtgc tcgaggccgc gatccggctg
     2521 accggcaacg aggtccgcca ccacgccgag ctggtgcgcg agctcggtcc ggtcccgctg
     2581 gtcgtcgccg acgacggccg gctgacccag gtgttcatca acctgctggt caacgccgcg
     2641 cacgcgatcc cggcgggccg gtcgagcgac aaccgcatca cggtgcggac ccggaccgac
     2701 gatctcggcc gcgccgtgat cgagatcgag gacaccggca ccggcatccc ggccgaactc
     2761 cagtcgcgcg tgttcgatcc gttcttcacc accaagggcg tgggcgaggg caccggcctc
     2821 ggcctgtcga tctgccacgg catcatcagc ggtctcggcg gccagatctc gatcgagagc
     2881 gcgccgacgc gcggcacggt ggtgcgcatc gtgctgccgc cgcacgtcgg cgacgaggtg
     2941 gccgaggcag cgccggcggt ggtcgccgag ccggccg
//
LOCUS       DASVOR010001903         1955 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_546164,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001903 DASVOR010000000
VERSION     DASVOR010001903.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546164"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..739
                     /locus_tag="VF516_35290"
     CDS             167..739
                     /locus_tag="VF516_35290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113066.1"
                     /translation="MKLAVTLAILTVTGCATAIDQDTSTTDQAAIVPTGLPSTSTTCQ
                     WVVDKTCYGQLNDGNNLWPTGIGITSMFVMTRSGPTRLSGRQQTYLAFVVWNNSVVGR
                     IFRLDVGSSDAANFTAVLGNIVAGRTLNLFDTTAGQGGATVGSPSPPPHPNVEGPITF
                     DSTYLGTVANDAASMNRATLDFLATKSVID"
     gene            821..>1955
                     /locus_tag="VF516_35295"
     CDS             821..>1955
                     /locus_tag="VF516_35295"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8113067.1"
                     /translation="MRCAMRMACAVGIAVVALRPAAAAPASPQQQIALIAKPSVMRVL
                     GVYVGTFVTPAGQFHESLGGSGTGFFITADGYIATNAHVVAPIHDGDTKARDALRRAL
                     FAELDHKYLAQLQKLTRAQLSDFISRIELVELKKLAYVVLPNGDHLDYEIKQFGAPGT
                     GRDCAVIKVKTSNAPSLPIGDSSKSQVEDPIVVLGYPGVADFEELLDAKSQLEATVTD
                     GAISSLKRAASGEPILQISAPITHGNSGGPAIDQHGAVIGLSTFGNQGEVQGFNFLVA
                     SATLMELVKDARLDLRPSPTSEAWRAGLEHYWNDEYTAAIARFEEVETAFPAHSEAPH
                     LIRLSHEAQKDGKEKQTGVGAGLAAGLILGGFAAIGAALWIARR"
BASE COUNT          315 a          697 c          656 g          287 t
ORIGIN      
        1 cgccggcatt cttcgacgcc gtgcgcgaca aggtccgcac cggcgcgtcg gtcgcggtcg
       61 cgctgcgcga cgtgcgccgg caatggctcg gcgaccgccg cgccgactgg gtccgcgatg
      121 tgatcgtgtt cgaatgaacc cgtagctgtt tgctcgaaag gaagttatga agctcgccgt
      181 cacgctcgcc atcctcactg tcaccggttg tgccactgcg atcgatcagg acacctccac
      241 gaccgaccag gccgccatcg tcccgaccgg gctgccgtcg accagcacga cctgccagtg
      301 ggtggtcgac aagacctgct acggtcagct caacgacggc aacaacctgt ggccgacggg
      361 aatcgggatc accagcatgt tcgtgatgac gcgctcgggg ccgacccggc tcagcggccg
      421 ccagcagacg tacctggcgt tcgtggtgtg gaacaactcg gtggtcgggc ggatcttccg
      481 gctcgacgtc ggcagctccg acgccgccaa cttcaccgct gtgctcggga acatcgtcgc
      541 cgggcggacc ttgaacctgt tcgacaccac ggcgggtcag ggcggcgcga cggtcggcag
      601 cccgtcgccg ccgccgcacc ccaacgtcga gggtccgatc acgttcgatt ccacctacct
      661 cggcaccgtg gccaacgacg cggcgagcat gaaccgcgcc accctcgact tcctggcgac
      721 caagtccgtg atcgactgac ctcgggtcga cgttcggctc accccgccgg ggcgaacccg
      781 tccgcaccgg cccgccggga atacgctacg atggggcctg atgaggtgcg ccatgcggat
      841 ggcctgcgcg gtcgggatcg cggtggtggc gctccgcccc gcggccgccg cgccggccag
      901 tccgcagcag cagatcgcgc tgatcgccaa gccgtcggtg atgcgcgtgc tcggcgtcta
      961 cgtcggcacg ttcgtcactc cggccgggca gttccatgag tcgctcggcg gcagcggcac
     1021 cggcttcttc atcaccgcgg atggctacat cgcgaccaac gcgcacgtgg tcgccccgat
     1081 ccacgacggc gacaccaagg ccagggacgc gctccggcgc gcgctgttcg ccgagctcga
     1141 tcacaagtac ctcgcccagc tgcagaagct gacgcgcgcc cagctcagcg acttcatcag
     1201 ccggatcgag ctggtcgagc tcaagaagct cgcctacgtc gtgctgccca acggcgatca
     1261 cctcgactac gagatcaagc agttcggcgc ccccggcacg gggcgcgact gcgcggtgat
     1321 caaggtcaag accagcaacg ccccgagcct gccgatcggc gactcgtcga agtcgcaggt
     1381 cgaggatccg atcgtcgtgc tcggctaccc gggcgtcgcc gacttcgagg agctgctcga
     1441 tgcgaagtcg cagctcgagg cgacggtcac cgacggcgcg atctcgtcgc tcaagcgcgc
     1501 ggccagcggc gagccgatcc tccagatctc ggcgccgatc acgcacggca actcgggcgg
     1561 cccggcgatc gaccagcacg gcgcggtcat cggactgtcg acgttcggca accagggcga
     1621 ggtccagggc ttcaacttcc tggtcgcgtc cgcgacgctg atggagctgg tcaaggatgc
     1681 ccggctcgat cttcgcccca gcccgaccag cgaggcgtgg cgcgccggcc tcgagcacta
     1741 ctggaacgac gagtacaccg cggcgatcgc caggttcgag gaggtcgaga ccgcgtttcc
     1801 agcccactcc gaggcgccgc acctgatccg gctgtcgcac gaggcccaga aggacggcaa
     1861 ggagaagcag accggcgtcg gggccgggct cgcggcgggg ctgatcctcg gcgggttcgc
     1921 ggcgatcggc gcggcgctgt ggatcgcgcg gcgat
//
LOCUS       DASVOR010001904         5061 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_165780,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001904 DASVOR010000000
VERSION     DASVOR010001904.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5061
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165780"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1497)
                     /locus_tag="VF516_35300"
     CDS             complement(163..1497)
                     /locus_tag="VF516_35300"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8113068.1"
                     /translation="MKHRPTRRRFLQGAAAAAGGALLLRGSRSDAGLLDLLPLPELSG
                     IQHIVVVMMENRSFDHLLGWLPGANGRQAGLSYPDASGVLHPTFPLAPTIGYQGCNNL
                     DPDHSWEGGRIQYDNGAMDGWLRAHSDLFSIGYYERADRPFFNELAASYTTLDRYHCS
                     ILAETFPNRFFAHAAQTPTLHNVGNTSGITDPQTIPTIWDRLAAAGVSGRYYFSDVPF
                     LGLWGDRYLSISRPYATFLAEAATGLLPAVSFVDPRFVDEGSGSSGDDHPHADLRVGD
                     AFLSQTFAAVARGPCWPSTVFIVTYDEWGGFFDHVAPPTSVAANAVDPPDASGNVLLG
                     MRVPVVVASPWTRAGGQPRISSALYDHTSILKLIEWRWNLPNLTPRDAPGSNITNLAA
                     ALDFAHPDPAVPPLPLLVTPVLDACAPVLPAVDDQWQRLLRSPLLASWHLPL"
     gene            complement(1565..1777)
                     /locus_tag="VF516_35305"
     CDS             complement(1565..1777)
                     /locus_tag="VF516_35305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113069.1"
                     /translation="MTRSAGHCSGPLAGELPDPADRMRVAPAIIGGLTLVTADAVLLD
                     RKLRGVRTRDASERCGGDHARWRHTR"
     gene            2059..2544
                     /locus_tag="VF516_35310"
     CDS             2059..2544
                     /locus_tag="VF516_35310"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8113070.1"
                     /translation="MPNPNPSIVTKGFWIFAKKFCSQCEQPLDKSWDQCPFCAQSVPP
                     VAAAPAKMKTIMTQAFGVEGVRMLGWIVPLQGAKRGELLTLSPVTTVGTDPTCTIMLD
                     DRFMSSRHAEIKAENGVWVLRDSGSTNGTYVNNRRIDRTELVDNDLLKFGNAILKFKS
                     L"
     gene            complement(2531..2878)
                     /locus_tag="VF516_35315"
     CDS             complement(2531..2878)
                     /locus_tag="VF516_35315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113071.1"
                     /translation="MLGEASTKLLGAGRIAVGLEDARAGGEPSQQAQALALHLTSWVG
                     LVTKVGVLGLGIDRILRDSDAERAEGGLSDLLGELIETIPHQRRQSATRMWIAATSAI
                     RIHHTRLCWVIGS"
     gene            3201..3530
                     /locus_tag="VF516_35320"
     CDS             3201..3530
                     /locus_tag="VF516_35320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113072.1"
                     /translation="MRASAPSPSRSCYKFQHMLGQSPTQDPNEQEFIMAMLKSWEEGK
                     AEARAEGQAEGQAKSILTVLRIRGIEVPPAARERILAQKDIDQLARWLEKATVASTIG
                     EVIDEPR"
     gene            complement(3550..4575)
                     /locus_tag="VF516_35325"
     CDS             complement(3550..4575)
                     /locus_tag="VF516_35325"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8113073.1"
                     /translation="MRVLVTGHRGYIGVELVPLLRQAGFDAVGLDTGFFDDSDFRSPP
                     DDIPALDLDLRDVTADHLRGFGAVVHLGALSNDPLGDLNPSLTYDINLHASVNLAKAA
                     KAAGVGRFVFASSCSLYGAGADGHLTEQAAFNPVTPYGESKVKTEIEVGALTDASFAP
                     VYLRNATAYGVSRRLRADIVVNNLVGYALTTGKVMMMSDGTPWRPLVHIGDISRAAIA
                     ALTAPADAIRNQAFNIGRTQENFRIREVAELVAQVVPNCEVSFAAGASPDARNYRVDF
                     TKAERSLPGYTPQWTLRAGIEELYAAYKGHGMTEAEFLGPRYYRLKVIRGRLERGELD
                     PNLRWLA"
     gene            complement(4583..>5061)
                     /locus_tag="VF516_35330"
     CDS             complement(4583..>5061)
                     /locus_tag="VF516_35330"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX8113074.1"
                     /translation="ARAAAAELAAAAARLTVDIHGFRDSFLPWDQPVRLKETLAELRR
                     SFEPDLVFTHHRADLHQDHALIAQLTWTAFRDHLILEYEIPKYEGDLGRPNVYVPLDE
                     AVARRKIDLILRHYATQAGKGWFRAETFEAVLRLRGIECNAPSGWAEAFHGRKILL"
BASE COUNT          818 a         1614 c         1871 g          758 t
ORIGIN      
        1 gcacctacca cttcggggtc tacgtctatc gctacgacat ccagaagaac tacgacacgc
       61 tgaccccggc gaagacgttc tacgtccgct cgctgatcga cgtccgcggc gtcgctcacc
      121 tcgagcccga ggtgctcggc caggacaagt cgtagccggc ggtcacagcg gcaggtgcca
      181 gctggcgagc aggggcgagc gcagcaggcg ctgccactga tcgtcgaccg ccggcagcac
      241 cggcgcgcag gcatcgagca ccggggtgac cagcagcggc agcggcggga ccgccgggtc
      301 ggggtgcgcg aagtcgagcg ccgcggccag gttggtgatg ttcgacccgg gcgcgtcgcg
      361 cggcgtcagg ttgggcaggt tccaccgcca ctcgatcagc ttgaggatcg aggtgtggtc
      421 gtacagcgcc gacgagatcc gcggctggcc gccggcgcgc gtccagggcg aggcgaccac
      481 gaccggcacc cgcatgccga gcagcacgtt gccgctggca tccggcggat cgacggcgtt
      541 cgcggcgacc gaggtcggcg gcgcgacgtg gtcgaagaac ccgccccact cgtcgtaggt
      601 gacgatgaac acggtgctgg gccagcacgg gccgcgggcg accgccgcga acgtctggct
      661 gaggaacgca tcgccgacgc ggaggtcggc gtgcgggtgg tcgtcgcccg agctgcccga
      721 gccctcgtcg acgaaccgcg ggtcgacgaa cgacaccgcc ggcaacaggc cggtcgcggc
      781 ctcggcgagg aacgtcgcgt agggccgcga gatcgacagg tagcgatcgc cccacaggcc
      841 gaggaacggg acatcggaga agtagtagcg gcccgacacg ccggccgcag cgagccggtc
      901 ccagatcgtc gggatggtct gtggatcggt gatcccgctc gtgttgccga cgttgtgcag
      961 ggtcggcgtc tgcgcggcgt gcgcgaagaa ccggttgggg aacgtctcgg cgaggatcga
     1021 gcagtggtag cggtcgagcg tggtgtagct cgcggcgagc tcgttgaaga acggccgatc
     1081 ggcgcgctcg tagtagccga tcgagaacag gtcgctgtgg gcgcgcagcc agccgtccat
     1141 cgcgccgttg tcgtactgga tccgtccgcc ctcccagctg tgatcgggat cgaggttgtt
     1201 gcagccctgg tatccgatcg tcggtgccag tggaaacgtc ggatgcagga cgccgctggc
     1261 gtcggggtag ctgagcccgg cctgccgtcc gttggcgccg ggcagccagc cgagcaggtg
     1321 atcgaagctg cgattctcca tcatcacgac cacgatgtgc tggatgcccg atagctccgg
     1381 cagcggcagc agatcgagca ggccggcgtc gctgcgcgag ccgcgcagca gcagcgcgcc
     1441 gccggcggcc gcggccgcac cctggaggaa ccgtcgtctg gtgggtcggt gcttcatgga
     1501 cagaacagct tgtgccgttc gtggccgcca cggccagccc gccgggcgcc gcttaacgcc
     1561 gggatcaccg ggtgtggcgc catcgcgcgt gatcgccgcc gcatcgctcg ctggcgtcgc
     1621 gggttcgaac cccgcgcagc ttccggtcga gcagcaccgc gtccgccgtc accagcgtga
     1681 gacccccgat gatcgccggg gcgacccgca tccgatccgc cggatccggg agttcaccgg
     1741 ccagtggacc cgagcaatgg cccgcggaac gtgtcatccg accagtgtca cgccttcggt
     1801 gacacggtgt ctcccgcgcg gacagtggag atctacagcg cagtcattgc cacttcgtgc
     1861 ctcccgatgc tcgatgtggc cgactggcgg tagatcgatc gcggcgcatg tgcaatgctg
     1921 gtgttcgcgg cgcacagggg gatgcgggaa gctcacccag gtgggctcgc agcagaggga
     1981 ggatagatgt cgcagcaggg attatcgccg gggatgcccc agatgtcggg catgccccag
     2041 gtgccgggta tgcctcagat gcccaacccg aacccgtcga tcgtcacgaa ggggttctgg
     2101 atcttcgcca agaagttctg ctcgcagtgc gagcagccgc tcgacaagag ctgggaccag
     2161 tgcccgttct gcgcgcagag cgtcccgccg gtcgccgccg cgccggcgaa gatgaagacg
     2221 atcatgaccc aggcgttcgg ggtcgagggg gtgcggatgc tgggctggat tgtcccgctg
     2281 cagggcgcga agcgcggcga gctcctcacg ctgtcgccgg tgaccaccgt gggcaccgac
     2341 ccgacctgca cgatcatgct ggacgatcga ttcatgtcgt cccgccacgc ggagatcaag
     2401 gccgagaatg gcgtgtgggt gctgcgcgac tccggatcga ccaatggcac ctacgtcaac
     2461 aaccgtcgca tcgatcgcac ggagctggtg gacaacgacc tcctcaagtt cggcaacgcg
     2521 atcctgaagt tcaagagcct atgacccagc acaaccgagt gtggtggatc ctgatcgcgc
     2581 tcgtggcggc aatccacatg cgcgtggcac tctggcggcg ctgatgcgga atcgtttcga
     2641 tcaactcgcc aagcagatcg gacaagccgc cctcggcccg ttcggcatcg ctgtcacgca
     2701 ggatgcgatc aatcccgaga cccagtacgc cgaccttcgt tacgagcccg acccagctcg
     2761 tcaagtggag cgcgagcgcc tgggcctgct gggacggctc gccgccagcg cgtgcatcct
     2821 cgagacctac agcgatgcgc ccagcgccga ggagtttcgt gcttgcctca ccaagcatct
     2881 caccgtctgg cagcagcgcg tacgcgatgc gcgatcggag agcaagaagc ggaatgagcc
     2941 gcagcctcgc gacgagtccg tggcgtcgtt cctctggatc atcgccgccg gcgcgccgac
     3001 cacgctcctg gccaagctca agctcgaatg ctcggcggac tggccggtgg ggatctacct
     3061 gttcggggac gacgtcctgc gcgtcggaat cgtcgtcgcc agccagctgc cacgcgatcg
     3121 cacgaccctg ctggtacgcc tgatggccgc agggccgctc ctggcgctcg cggtcaagga
     3181 agtcgctgca cttccgcccg atgcgtgcga gcgcgccgtc gccgagccga tcctgctaca
     3241 agttccagca catgctcggg cagagcccga cccaggaccc caacgagcag gagttcatca
     3301 tggccatgct gaagagctgg gaggaaggaa aagccgaggc acgagccgag ggacaggccg
     3361 agggacaggc caagtccatc ctcaccgtgc ttcgcattcg ggggatcgag gttccaccgg
     3421 ccgcgcgcga gcgcatcctg gcccagaagg acatcgacca gctcgcgcga tggctcgaga
     3481 aggccaccgt tgcctcgacg atcggagagg tgatcgacga gccgaggtga gcggcgcgag
     3541 aggcgactcc tacgcgagcc agcgtagatt ggggtcgagc tcgccgcgct cgaggcggcc
     3601 gcggatgacc ttgaggcggt agtagcgcgg gccgaggaac tcggcctcgg tcatgccgtg
     3661 gcccttgtag gcggcgtaga gctcctcgat gccggcgcgc agcgtccact gcggggtgta
     3721 gccggggagc gagcgctcgg ccttggtgaa gtcgacgcgg tagttgcggg cgtccggcga
     3781 cgcgcccgct gcgaagctga cctcgcagtt cgggacgacc tgggcgacca gctcggcgac
     3841 ctcgcggatc cggaagttct cctgggtgcg gccgatgttg aatgcctggt tgcggatcgc
     3901 gtcggcgggg gcggtgaggg cggcgatcgc cgcgcgcgag atgtcgccga tgtggacgag
     3961 cgggcgccac ggggtgccgt cgctcatcat catgaccttg ccggtggtga gggcgtagcc
     4021 gaccaggttg ttgacgacga tgtcggcgcg caaccggcgg gataccccgt aggcggtggc
     4081 gttgcgcagg tagaccggcg cgaagcttgc gtcggtgagc gcgccgacct cgatctcggt
     4141 cttgaccttg gactcgccgt agggcgtgac cgggttgaac gcggcctgct cggtgaggtg
     4201 gccatcggcg ccggcgccgt acaggctgca cgacgacgcg aacacgaagc ggccgacgcc
     4261 ggcggccttg gcggctttgg cgaggttgac cgaggcgtgg aggttgatgt cgtaggtcag
     4321 gctcgggttg agatcgccga gcggatcgtt ggacagcgcg ccgaggtgga cgacggcgcc
     4381 gaagccgcgc aggtggtcgg cggtgacgtc gcgcaggtcg aggtcgagcg cggggatgtc
     4441 gtcgggcggc gaccggaagt cgctgtcgtc gaagaagccg gtgtcgaggc cgacggcgtc
     4501 gaaccccgcc tggcggagca gggggacgag ctcgacgccg atgtagccgc gatgaccggt
     4561 cacgaggacg cgcatgggcc gactacaaca gaatcttgcg gccgtgaaaa gcctcggccc
     4621 agccggacgg cgcgttgcac tcgatgccgc gcagccgcag cacggcctcg aaggtctcgg
     4681 cgcggaacca gcccttgccg gcctgggtgg cgtagtgtcg gaggatcagg tcgatcttgc
     4741 ggcgggcgac ggcctcgtcg agcgggacgt agacgttggg gcggccgaga tcgccctcgt
     4801 acttggggat ctcgtactcg aggatcaggt gatcgcggaa cgcggtccag gtcagctggg
     4861 cgatcagcgc gtggtcctgg tggaggtcgg cgcggtggtg ggtgaacacc aggtcgggct
     4921 cgaagctcct gcgcagctcg gcgagggtct ccttgagccg gaccggctgg tcccagggca
     4981 ggaagctgtc gcggaagccg tggatgtcga cggtgagccg ggcggcggcg gcggcgagct
     5041 cggcggcggc ggcgcgggcc t
//
LOCUS       DASVOR010001905         5427 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_428332,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001905 DASVOR010000000
VERSION     DASVOR010001905.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428332"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VF516_35335"
     CDS             complement(<1..228)
                     /locus_tag="VF516_35335"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113075.1"
                     /translation="MHSVAIFGAAGYAGLELVKLLAVHPGVRIACAASDARAGTPVDE
                     LTGTRTGAAFVTTREALAAEAEVALLATPPEA"
     gene            complement(431..769)
                     /locus_tag="VF516_35340"
     CDS             complement(431..769)
                     /locus_tag="VF516_35340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8113076.1"
                     /translation="MLSFQRLEVYQRSIEFLAHALEVIAELPSKGHAEIADQLRRAAQ
                     SQPLNIAEGAGRTSRPDVAKHYTIARGSAMECAAVLDVLKAQGTIKPHLYERGIDLLT
                     SIVAMLTKMI"
     gene            complement(905..1276)
                     /locus_tag="VF516_35345"
     CDS             complement(905..1276)
                     /locus_tag="VF516_35345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113077.1"
                     /translation="MLAGIADRRRMTGTAHSAPTISEPLTWEQICKRYPDQWVGLVEF
                     DRIDPGRFAFRTARVVGHDPRRRTAVEQSRPWWRAYEEVGQHFTGRTEARLWMAPRGP
                     VRIDRPWDRAGTATTLLAPPS"
     gene            complement(1337..1810)
                     /gene="smpB"
                     /locus_tag="VF516_35350"
     CDS             complement(1337..1810)
                     /gene="smpB"
                     /locus_tag="VF516_35350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEX8113078.1"
                     /translation="MAKPAPDAIRVIASNRKATHDFHIHERMEAGIALVGSEVKSLRE
                     AKVTIADGWVEIRKGQAYVHGIQINEYAQANRWQHDVSRVRKLLLHKHEIDKLGAKTT
                     IQGYTLIPLGLYFKGPRVKVELALVTHKKQHDKRAAKREADDRREIDRAMKGNRA"
     gene            complement(1810..2235)
                     /locus_tag="VF516_35355"
     CDS             complement(1810..2235)
                     /locus_tag="VF516_35355"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8113079.1"
                     /translation="MRYLHTMIRVRDLPAAVHFFCEGLGLTEVRRRDHEQGRFTLVFL
                     GERGEGDSPQLELTYNWDQKDPYTVGRNFGHVAYEVNDIYAACQRFLNAGYQISRPPR
                     DGRMAFIRSPDLISVELLQKGAALAPVEPWTSMPNVGEW"
     gene            2274..2687
                     /locus_tag="VF516_35360"
     CDS             2274..2687
                     /locus_tag="VF516_35360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113080.1"
                     /translation="MSEPNALVNFRLDRDRPMRIKWLATDGIPGDGYKAQVMTIKLDD
                     GSVLEMDSSAILEQTAPDPAGGIGAYLVTFNGMVGYASDHPDRPRIDQLEDEEIGYEM
                     AFIRERDGQLAVEGEDYEIRDRPRGMAHKLTRRHA"
     gene            2680..3555
                     /locus_tag="VF516_35365"
     CDS             2680..3555
                     /locus_tag="VF516_35365"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEX8113081.1"
                     /translation="MRDDAPSRTAQWVAAARGMGRLLPEAVRIADDPYGLSFASPSIA
                     RLVDRTRDAHPDRAQVIARVPGLSLWIAYMQVRTRLIDDAVRAFATAGGHQVVVLGAG
                     YDCRALRLPELAGARVFEVDHPATQGHKRAVLDRLGARSPARYLTWDFETHPMDDLPG
                     ALADAGHDPAAPTLTIWEGVTMYLTDAAIHASLRAIASWSTAGSELALTYMTRQRLQR
                     SVALRAVRAVVRSLGEPFRFGWDPDELPGYLAARGFALTSDVSTADAAHQLLPPELAA
                     QIVQTDRVAIARRAP"
     gene            3636..4781
                     /locus_tag="VF516_35370"
     CDS             3636..4781
                     /locus_tag="VF516_35370"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8113082.1"
                     /translation="MRVLVIGGGIFGVAAARELRARGCSVTLADPGPLPHPLAESTDI
                     SKIVRCDYGADEDYTALGERALDGWRRWNAAWAVPRFHETGVAFLTRSPMQAGEFEHD
                     SFALLSRRGHHVERLDAAAIAARFPAYRPGAYVDGYFHREGGWAESGAVVAHLLVEAI
                     TAGVTVLGDCPIHRIVDGGALSGSALLPAEAIVVCAGAWTPLLVPELASELRAVGQPV
                     FHLRPTDRALFADLQFPVFGADIARTGYYGFPIQHDGIVKIANHGAGIALRLDAPRQV
                     TPDQESALRDFLRGSLPALADAPIASRRLCVYCDSRDADFWIARHPHRPGLTVATGGS
                     GHGFKFAPVIGPIIADAVLGIDHPLAHKFRWRAGAEPARRGDAARSS"
     gene            4865..>5427
                     /locus_tag="VF516_35375"
     CDS             4865..>5427
                     /locus_tag="VF516_35375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8113083.1"
                     /translation="MKAVVLTGYGDVDQLELRELPAPRVGPGELSVRVAGASINPIDW
                     KIRRGELRGVMPIELPVILGRDASGEVIEVGPGARGFAIGARVMGYVQSAYAERVVAK
                     AESWAIVPTQVDLIDAAAIPLVGLTGSQLIDAIDPAAGQTLLVTGAAGSVGRVAVFAA
                     RARGAHVWAGVRRTQKQDVAHLAVDHIV"
BASE COUNT          743 a         2024 c         1826 g          834 t
ORIGIN      
        1 ggcctcgggc ggcgtcgcga gcagcgccac ctcggcctcg gccgccagcg cctcccgcgt
       61 ggtcacgaac gccgccccgg tccgcgtccc ggtcagctcg tcgaccggcg tgcccgcccg
      121 ggcatcgctc gccgcgcacg cgatccgcac gcccgggtgc accgccagga gcttgaccag
      181 ctccagcccg gcgtacccgg cggcgccgaa gatcgcaacg ctatgcatcg actgcatgtt
      241 tatgcatgcc ggcgccgaga atgcaagccc cgcgacggcg cacgaaccgt cacccgggcg
      301 tcacggtggc gctcggagcg caagcggtgc ttgacgttga cggtttgacg tcgacgtcga
      361 cgtcgaccca gaacgttgac gttgagggcg acgtacgacg ttgcccccac cgtcgacctc
      421 gacctcgacc tcagatcatc ttcgtcaaca tcgcgacgat gctggtcagc aagtcgatac
      481 cgcgctcgta gaggtggggc ttgatcgtgc cctgggcctt caggacgtcg aggacggcag
      541 cacattccat ggccgagccg cgagcgatcg tgtagtgctt cgcgacgtct ggacgcgatg
      601 ttcgacccgc accctcggcg atgttgagcg gctgcgactg ggcggcgcgt cgcaactgat
      661 cggcgatctc cgcgtggccc ttcgagggaa gctccgcgat gacctcgagc gcgtgggcga
      721 ggaactcgat cgagcgctga tagacctcga gacgctgaaa gctgagcatg cagtctgtat
      781 cggctccgcc ggccgaaggt tgcttcactc cggagtcaac gtgcaaggtc aagggtgggg
      841 tcagacgact acgttgccgt caacctcaac ggtggggtcg aggtcggggt ccacgtccag
      901 ggtgtcatga cggcggcgcg agcagcgtcg tggctgtgcc agcacgatcc cacggccggt
      961 cgatgcgaac gggacctctc ggagccatcc acagtcgagc ctcggtgcgc cccgtgaagt
     1021 gttgcccgac ctcctcgtag gcccgccacc agggccgcga ttgctcgacc gccgtgcggc
     1081 gacgcgggtc gtggccgacc acgcgagcgg tgcgaaacgc aaagcggccg gggtcgattc
     1141 gatcgaactc gaccagaccc acccattgat ccggatagcg tttgcagatc tgctcccagg
     1201 tcagtggctc ggagatcgtc ggtgcggaat gcgctgtgcc cgtcatgcgt cgacgatcgg
     1261 cgatccctgc cagcaccgca aaataattgg caattttgca gcatcgcgac agctcgtgtc
     1321 gcgatgctcc cagcgatcaa gcgcggttgc ctttcatcgc gcgatcgatc tcgcgccgat
     1381 cgtcggcctc ccgcttggcg gcgcgcttgt cgtgctgctt cttgtgggtc accagcgcga
     1441 gctcgacctt gacccgcggg cccttgaagt agaggccgag cgggatcagc gtgtagccct
     1501 ggatcgtggt cttggcgccg agcttgtcga tctcgtgctt gtggagcagc agcttgcgga
     1561 cgcgcgagac gtcgtgctgc cagcggttgg cctgcgcgta ctcgttgatc tggatgccgt
     1621 ggacgtaggc ctggcccttg cggatctcga cccagccgtc ggcgatggtg accttggcct
     1681 cacgcaggct cttgacctcg gagccgacga gcgcgatgcc ggcctccatc cgctcgtgga
     1741 tgtggaagtc gtgcgtcgcc ttgcggttgc tggcgatcac gcggatcgcg tcgggggccg
     1801 gcttggccat caccactcgc cgacgttggg catcgaggtc cacggctcga cgggagcgag
     1861 cgcggcgccc ttctggagca gctcgacgga gatcaggtcg ggcgagcgga tgaacgccat
     1921 gcgaccgtcg cgcggcggcc ggctgatttg atagcccgcg ttgaggaacc gctggcacgc
     1981 ggcatagatg tcattgacct cgtacgccac gtgaccgaag ttgcggccga cggtgtatgg
     2041 gtccttctgg tcccagttgt acgtgagctc gagctgcggc gaatcgccct cgccgcgctc
     2101 gccgaggaac accagcgtga agcgaccctg ctcgtggtcg cggcgccgga cctcggtcag
     2161 cccgagcccc tcgcagaaga agtggaccgc cgcgggcagg tcgcggaccc ggatcatcgt
     2221 gtgcaggtag cgcataggcg tttcgttacc agattttggg tattctgcgc gcgatgagcg
     2281 agcccaacgc cctcgtgaat tttcgcctcg accgcgaccg cccgatgcga atcaagtggc
     2341 tggcgaccga cggcatcccg ggagatggct acaaggcgca ggtcatgacg atcaagctcg
     2401 acgacggctc ggtcctggag atggacagct cggcgatcct cgagcagacc gctccggatc
     2461 cggcgggtgg catcggcgcc tacctggtga cgttcaacgg catggtcggg tacgcgagcg
     2521 accaccccga ccggccgcgg atcgaccagc tggaggacga ggagatcggc tacgagatgg
     2581 cgttcatccg cgagcgcgac ggccagctcg ccgtcgaggg cgaggactac gagatccgcg
     2641 accgcccgcg cggcatggca cacaagctga cccgccgcca tgcgtgacga cgcgccgagc
     2701 cgcaccgccc agtgggtcgc ggccgcgcgc ggcatgggcc ggctgctgcc cgaggcggtc
     2761 cggatcgccg acgaccccta cggcctttcg ttcgcctcgc cgagcatcgc ccggctggtc
     2821 gaccgcaccc gcgacgccca ccccgaccgc gcgcaggtca tcgctcgcgt ccccggcctg
     2881 tcgctgtgga tcgcgtacat gcaggtccgc acccggctga tcgacgacgc ggtgcgcgcg
     2941 ttcgccaccg ccggcggcca ccaggtcgtc gtgctcggcg ccggctacga ctgccgcgcg
     3001 ctgcgcctgc ccgagctcgc cggtgcgcgc gtgttcgagg tcgaccaccc ggcgacccag
     3061 ggccacaagc gcgcggtgct cgatcggctc ggcgcccgct cgcccgcccg ctacctcacg
     3121 tgggacttcg agacccaccc gatggacgac ctgccgggcg cgctcgccga cgccggccac
     3181 gaccccgcgg cgccgaccct gacgatctgg gagggtgtga cgatgtacct caccgacgcg
     3241 gcgatccacg cctcgctgcg cgcgatcgcg agctggagca cggccggctc ggagctcgcg
     3301 ctgacctaca tgacgcggca gcggctgcag cgttcggtgg cgctgcgcgc cgtgcgggcg
     3361 gtcgtgcgca gcctcggcga gccgttccgg ttcggatggg atcccgacga gctgcccggc
     3421 tatctcgccg cgcgcggctt cgcgctgacc agcgatgtgt ccaccgccga cgccgcgcat
     3481 cagctcctgc cgcccgagct cgccgcacag atcgtgcaga ccgaccgggt cgcgatcgcg
     3541 cgccgcgcgc cgtagcggct tctctcgctc gtctctcgca gccggccgcc catcgccctt
     3601 gtcggaccgc gcgccgcccg ggatacagtg cgcccgtgcg cgtcctcgtg atcggcggcg
     3661 gcatcttcgg cgtggccgcc gcccgggagc tccgcgcccg gggctgcagc gtcaccctcg
     3721 ccgatcccgg cccgctgccc catccgctcg ccgagtccac cgacatcagc aagatcgtcc
     3781 gctgcgacta cggcgccgac gaggactaca ccgcgctcgg cgagcgcgcg ctcgacggct
     3841 ggcgccggtg gaacgccgcc tgggcggtcc cccggttcca cgagaccggg gtcgccttcc
     3901 tcacccgcag cccgatgcag gccggcgagt tcgagcacga cagcttcgcc ctgctgtccc
     3961 gccgcggcca tcatgtcgag cggctcgacg ccgccgcgat cgccgcgcgg ttcccggcct
     4021 accgccccgg cgcctacgtc gacggttact tccaccgcga gggcggctgg gccgagtccg
     4081 gcgccgtggt cgcccacctc ctggtcgagg cgatcaccgc cggcgtcacc gtcctgggcg
     4141 actgcccgat ccaccgcatc gtcgacggcg gcgcgctgtc gggctcggcc ctgctccccg
     4201 ccgaggcgat cgtcgtgtgc gccggggcgt ggaccccgct cctggtcccc gagctcgcca
     4261 gcgagctccg cgccgtgggc cagccggtgt tccacctgcg gcccaccgac cgcgcgctgt
     4321 tcgccgacct ccagttcccg gtgttcggcg ccgacatcgc ccgtaccggc tattacggtt
     4381 ttccgatcca gcacgatggc atcgtcaaga ttgccaatca cggcgccggc atcgcgctcc
     4441 gcctcgacgc gccccgtcag gtcacgccgg atcaggaatc cgcgctacgc gactttctgc
     4501 gcggctcgct gccggcgctc gccgacgccc cgatcgcgtc ccgccggctg tgcgtctact
     4561 gcgacagccg cgacgccgac ttctggatcg cccgccaccc ccaccgcccc ggcctcaccg
     4621 tcgccaccgg cggctccggc cacggcttca agttcgctcc cgtgatcggc ccgatcatcg
     4681 ccgacgccgt gctcgggatc gaccacccgc tcgcccacaa gtttcgctgg cgcgccggcg
     4741 ccgagccggc acggcgcggc gacgccgccc gctcgtcgta gccgcgcacc accgcacgcc
     4801 gccgtgcgct cctgacaccg ccgtcccgac gagccttgcg cgcgatcgct ggcatcctgt
     4861 cgtcatgaag gccgtcgtgc tcaccggata tggcgacgtc gaccagctcg agctccgcga
     4921 gctgcccgct ccccgggtcg gccccggcga gctcagcgtc cgcgtcgccg gcgccagcat
     4981 caacccgatc gactggaaga tccgccgcgg cgagctccgc ggggtgatgc cgatcgagct
     5041 ccccgtgatc ctcggccgcg acgcgtccgg tgaggtcatc gaggtcggcc ccggcgcccg
     5101 cggcttcgcg atcggcgccc gcgtcatggg ctacgtccag agcgcctacg ccgagcgcgt
     5161 cgtcgccaag gccgagtcgt gggcgatcgt cccgacccag gtcgacctga tcgacgccgc
     5221 cgcgatcccg ctggtcggcc tcaccggctc ccagctgatc gacgcgatcg accccgccgc
     5281 cggccagacc ctgctcgtca ccggcgcggc cggcagcgtc ggacgcgtcg cggtgttcgc
     5341 cgcccgcgcg cgcggcgccc acgtctgggc cggcgtccgg cggacccaga agcaggacgt
     5401 cgcccacctc gccgtcgacc acatcgt
//
LOCUS       DASVOR010001906         4637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_195508,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001906 DASVOR010000000
VERSION     DASVOR010001906.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_195508"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1768
                     /locus_tag="VF516_35380"
     CDS             104..1768
                     /locus_tag="VF516_35380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX8113084.1"
                     /translation="MAAGALVLTSCATAEREDNGDRQADELGSIQQQPHSKVCVGGRF
                     ACKAQVRTDAQNHIKPFATPSGLGPADLASAYKLDPTKTSTATIAIVDAFNYPNAESD
                     LATYRSQFGLSPCTKANGCLKIVNQSGATSPLPGNSPSGDDWTVETALDLDMASAACP
                     TCKLLLVEAQDDQGDGLFQAQNAASAVPGVVAISDSWGGPSDGTDVSSDAQFFTHTGV
                     SIFVATGDNGVNQKPDWPSTSPHVIGVGGTTLTKSSNARGWVEGAWSGAGSSCSTLIG
                     KPSFQSVVPSAACAKRAASDVAAVADPNTGLAVFNAANGGWIVVGGTSAASPFVAGVY
                     ARYGIAGTNDASFAYAHAGQFFDVTTGKNGSCGGALCNAGAGWDGPTGLGTPNGAVLS
                     GGGGGGCTPKCTGKSCGDDGCGGSCGTCGSGQTCSPGGVCTTGGSCNHPICSAGGALT
                     SSCDTCAAQICAQDSFCCNSSWDSICVGEVQSICKQSGTGGGGGGGGGGGNTCAHSLC
                     NQGTKLTSSCDSCAAQICAADSFCCNNKWDGQCVGEVSSICNESCN"
     gene            complement(1885..2160)
                     /locus_tag="VF516_35385"
     CDS             complement(1885..2160)
                     /locus_tag="VF516_35385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEX8113085.1"
                     /translation="MCRNIRPLFNFEPPATDDEIRAAALQYVRKVSGTRKPSEANADP
                     FERAVEAIARATRRLVDSLETTAPPKSREVEAAKARARSAHRYTVTR"
     gene            complement(2170..3162)
                     /gene="ligD"
                     /locus_tag="VF516_35390"
     CDS             complement(2170..3162)
                     /gene="ligD"
                     /locus_tag="VF516_35390"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8113086.1"
                     /translation="MASLPIDTPLALRELQLATLVADPPAGSDWLHEQKFDGYRILAE
                     LDRGKVRLLSRRFKDWTAAFPEVADAVAGLPVRRAVLDGEVAAVLSDGRTSFQALQNR
                     NTANVAYFAFDLLAEDDDDLTRLPLEQRKARLQDLLSRPRRRAGVIRYSDHVVGGGRE
                     FFALACQRGLEGIISKRRDKPYLPGRGTTWVKTKCLQRQELVVGGFTDPEGSRQGIGA
                     LLVGYYAGPQLIYAGKVGTGYSHAMLVELRKRLTPIERATSPFSPEPPRAWTGASRHW
                     VAPELVAEVAFSEWTNDGRLRHPSFQGLRFDKPARDVVREAPARLGRSRRSGEG"
     gene            complement(3297..4427)
                     /locus_tag="VF516_35395"
     CDS             complement(3297..4427)
                     /locus_tag="VF516_35395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm1 family immunity protein"
                     /protein_id="HEX8113087.1"
                     /translation="MTSFLVPDDFRAFLRSVVEDLDAPGGSVRVDAEDAFQDACGYGG
                     RVAGAYRFHYLTRDGMQRWTIALAEHEVRAIADGLQIEADGECVEIAHRHHREPTGEP
                     LLIWGAYDDDALTVRSLDDLVAALEVLRLAALDAPRVLRVWSPSDDQLVAAIWRDHCA
                     LYVLESADGYATSSGDPRRTDAFEVLDHDGNRMVVRWGDCVAWPAACRALSRFAAHGD
                     LGPEIAVERRIPSQLLMHGELDRQAVLAMRAAPAGDPGSSSLVRLTAPAVPVALSDSV
                     DTTVPVEVAPVDLPALAAWARRLIELLFARALVELDDSPGIDELCYQLSGLLQAHGDE
                     AEHALDTAEWLANEIGALRGIRRLFATGGDLQIALRRSRVDR"
BASE COUNT          670 a         1683 c         1678 g          606 t
ORIGIN      
        1 ggcggcggcc tcggttgcac aagtcgcgca accaagcatg gcactgacga tgaacaagcc
       61 ggccgccatc atgaacgcca tcagatccat caccacgatt gccatggcgg ccggcgcgct
      121 cgtgctcacg agctgtgcga ccgccgaacg cgaggacaac ggagatcgcc aggccgacga
      181 gctcggatcg atccagcagc agccgcacag caaggtgtgc gtcggcggcc ggttcgcctg
      241 caaggcccag gtccgcacgg acgcgcagaa ccacatcaag ccgttcgcga cgccgtccgg
      301 cctcggcccg gccgacctcg cgtccgcgta caagctcgac ccgacaaaga cgtcgaccgc
      361 cacgatcgcg atcgtcgacg cgttcaacta tcccaacgcc gagagcgacc tcgccacgta
      421 ccgcagccag ttcggcctgt cgccgtgcac caaggcgaac ggctgcctga agatcgtcaa
      481 ccagagcggc gcgacgtcgc cgctgcccgg caactcgccg tccggcgatg actggacggt
      541 cgagaccgcg ctcgacctcg acatggccag cgccgcgtgc ccgacctgca agctcctgct
      601 ggtcgaggcg caggacgacc agggcgacgg cctgttccag gcccagaacg cggcctccgc
      661 ggtccccggc gtggtcgcga tcagcgacag ctggggcggc ccgtcggacg gcaccgacgt
      721 gtcgtcggac gcgcagttct tcacccacac cggcgtcagc atcttcgtgg cgaccggcga
      781 taacggcgtc aaccagaagc ccgactggcc cagcacgtcg ccgcacgtga tcggcgtcgg
      841 cggcacgacc ctcaccaagt cgagcaacgc gcgcggctgg gtcgagggcg cgtggagcgg
      901 cgcgggcagc tcgtgcagca cgctgatcgg caagccgagc ttccagtcgg tggttccgtc
      961 ggcggcgtgc gccaagcgcg cggcatccga cgtcgcggcg gtcgccgatc cgaacaccgg
     1021 cctcgcggtg ttcaacgccg cgaacggcgg ctggatcgtc gtcggtggca ccagcgcggc
     1081 cagcccgttc gtcgccggcg tgtacgcgcg ctacggcatc gccgggacca acgacgcgag
     1141 cttcgcgtac gcgcacgcgg gccagttctt cgacgtcacg accggcaaga acggcagctg
     1201 cggcggcgcg ctgtgcaacg cgggcgcggg ctgggacgga ccgaccggcc tcggcacgcc
     1261 gaacggcgcc gtgctcagcg gcggtggtgg cggcggctgc accccgaagt gcaccggcaa
     1321 gtcgtgcggc gatgacggct gcggcggcag ctgcggtacg tgcggcagcg gccagacctg
     1381 cagccccggc ggcgtctgca ccaccggcgg cagctgcaac cacccgatct gctcggccgg
     1441 cggcgccctc acctcgtcgt gcgacacctg cgccgcccag atctgcgcgc aggactcgtt
     1501 ctgctgcaac agctcgtggg actcgatctg cgtcggcgag gtccagtcga tctgcaagca
     1561 gtcgggcacc ggcggtggtg gtggtggtgg cggcggcggc ggcaacacct gcgcgcactc
     1621 gctctgcaac cagggcacga agctgacctc gagctgcgac agctgcgccg cccagatctg
     1681 cgccgcggac tcgttctgct gcaacaacaa gtgggacggg cagtgcgtcg gcgaggtctc
     1741 ctcgatctgc aacgagtcgt gtaactgagc cggcgctgct tcgtcgcggt gagcgagaag
     1801 cagctcgagt tcaccaccgc gcgcgccctg ctgcgagacg gccagggcgc gccggcgcgt
     1861 tcacgggacc ccagctcggc ccggctagcg cgtgaccgtg tagcggtggg cgctccgcgc
     1921 gcgggccttg gcagcctcga cctcgcggct cttgggcggc gccgtggtct cgagcgagtc
     1981 gaccagccgc cgcgtcgcgc gggcgatcgc ctcgaccgcg cgctcgaacg gatcggcgtt
     2041 ggcctcgctc ggcttgcgcg tgccgctgac cttgcggacg tactgcagcg ccgcggcgcg
     2101 gatctcgtcg tcggtcgcgg gcggctcgaa gttgaacagc gggcggatgt tgcggcacat
     2161 gccgccagtc taaccttccc cgctgcggcg cgacctgccg aggcgcgccg gggcctcccg
     2221 caccacgtcc cgcgccggct tgtcgaaccg caggccctgg aacgaggggt ggcgcagccg
     2281 gccgtcgttg gtccactcgc tgaacgcgac ctcggcgacc agctcgggcg cgacccagtg
     2341 ccgcgaggcg ccggtccagg cccgcggcgg ctcgggcgag aacggcgagg tcgcgcgctc
     2401 gatcggggtc agccgcttgc gcagctcgac cagcatcgcg tggctgtagc cggtgccgac
     2461 cttgccggcg tagatcagct gcggcccggc gtaatagccg acgagcagcg cgccgatgcc
     2521 ctgccggctg ccctcgggat cggtgaaccc gccgaccacg agctcctggc gctgcaggca
     2581 cttggtcttg acccaggtcg tgccgcggcc cggcaggtag ggcttgtcgc ggcgcttgga
     2641 gatgatgccc tccaggccgc gctggcacgc cagcgcgaag aactcgcggc cgccgccgac
     2701 cacgtgatcg ctgtagcgga tcacgccggc ccggcgccgc ggtcgagaca gcagatcctg
     2761 gagccgggcc ttgcgctgct cgagcggcag ccgcgtgagg tcgtcgtcgt cctcggccag
     2821 cagatcgaac gcgaagtacg cgacgttcgc ggtgttccgg ttctgcagcg cctggaagct
     2881 ggtccgcccg tcggacagca ccgcggcgac ctcgccgtcg agcaccgcgc ggcggaccgg
     2941 gagcccggcg accgcgtcgg cgacctcggg aaacgccgcg gtccagtcct tgaaccgccg
     3001 gctgagcagc cggaccttgc cgcgatcgag ctcggcgagg atgcggtacc cgtcgaactt
     3061 ctgctcgtgt agccagtcgg agccggcggg tgggtccgcg acgagcgtgg cgagctgcag
     3121 ctcgcgaagc gccagcggcg tgtcgatcgg cagggaggcc acgacggttt gtggccgggc
     3181 gtacgggatc gttcaagccc gatcccgatc ggcgacccgc gcgacgcgac gcgcggatgg
     3241 gtgaatgtcg cggcttcgcc gcgacaatcc ccagaggggg tcatcgggca agccccctat
     3301 cgatcgaccc gcgagcggcg gagtgcgatc tggagatcgc cgccggtcgc gaacagcctg
     3361 cggatcccgc ggagcgcgcc gatctcgttg gccagccact cggcggtgtc gagcgcgtgc
     3421 tcggcctcgt cgccgtgggc ctggagcagg ccgctcagct ggtagcacag ctcgtcgatg
     3481 ccggggctgt cgtcgagctc gaccagcgcg cgcgcgaaca gcagctcgat cagccggcgg
     3541 gcccacgccg cgagcgcggg caggtcgacc ggcgccacct cgaccggcac ggtggtgtcg
     3601 acgctgtcgg acagggcgac cggcaccgcc ggcgcggtca gccggaccag gctcgagctg
     3661 cccgggtcgc ccgccggcgc ggcccgcatc gcgagcacgg cctggcgatc gagctcgccg
     3721 tgcatgagca gctggctcgg gatccggcgc tcgaccgcga tctcggggcc gaggtcgccg
     3781 tgcgcggcga accggctcag cgcgcggcac gccgccggcc acgcgacgca atcgccccac
     3841 cgcacgacca tgcgattgcc gtcgtgatcg agcacctcga acgcgtcggt ccgccgcgga
     3901 tccccggacg aggtcgcgta gccgtcggcg gactccagca cgtacagcgc gcagtggtcg
     3961 cgccagatcg ccgcgaccag ctggtcgtcg ctcggcgacc acacccggag cacgcgcggc
     4021 gcgtcgagcg cggcgagccg gaggacctcc agcgcggcga ccaggtcgtc gaggctgcgc
     4081 acggtcagcg cgtcgtcgtc gtaggcgccc cagatcagga gcggctcgcc ggtcggctcg
     4141 cggtggtggc ggtgcgcgat ctcgacgcac tctccgtcgg cctcgatctg caggccatcg
     4201 gcgatcgcac ggacctcgtg ctcggccagc gcgatcgtcc agcgctgcat cccgtcgcgg
     4261 gtcaggtagt gaaagcggta cgcgccggcg acccgcccgc cgtagccgca cgcgtcctgg
     4321 aacgcgtcct cggcgtcgac ccgcacgctg ccgccgggcg cgtcgaggtc ctcgaccacc
     4381 gagcgcagga acgcgcgaaa atcgtcgggg accagaaacg acgtcatgac gcgccccgcc
     4441 ggcgaaggta tcaggccgcg cagcgaaagg tgaccgcaga tgccgcgccg cggccaccgg
     4501 gtgccgcacg tggcggcatg cgatcccccg tgcgtgtccg tcgcgcccgg cgaggcgtgt
     4561 ctggccctgt gtctggccgg gtacaccagc aagcaagcgt ccgggatgcc tcgactttgc
     4621 gcggggcgcg gtgcttg
//
LOCUS       DASVOR010001907         3164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_254145,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001907 DASVOR010000000
VERSION     DASVOR010001907.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254145"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            721..1425
                     /locus_tag="VF516_35400"
     CDS             721..1425
                     /locus_tag="VF516_35400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113088.1"
                     /translation="MVSRWLGVVLVVCACGGASGPVSPAAEGVRAYVRALRDNNPREA
                     YALLSAEARKKISFDEFALAWKQSDKERAWQARALDDGLRGNPDVGERALISFSDGKL
                     VQLEREGKTWRLESELVSRSRAKQPRDAIRLFAEAITARDITGVLNVLTQRRRDGLTR
                     QVEGFVAGIGKRINDRIDQFGTDRAELRWDENGIRYRIVLRKEEDEWRVDDIYIRPAP
                     KDDDGDKSEGSVPEDF"
     gene            complement(1598..2374)
                     /locus_tag="VF516_35405"
     CDS             complement(1598..2374)
                     /locus_tag="VF516_35405"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8113089.1"
                     /translation="MLHDDDYPWVMAQRESKRGGKQQPRQRELFRRGGKRRGAGRKPR
                     GARAGERHQARPDFKPYHALHVVVRVAPAVGSLRCRKLYKAMRDATITAALREWFRIV
                     HISLQRNHVHLLVEAEHKAALSRGMQGFLISAARHINTALGDGARRRRGKVFSDRYHV
                     EVITTPTRARHAISYVLGNWRKHGEDQAELPSTWLVDPFSSAIMFPDWQELDGQPCMW
                     PIREGYDPPMVRRPQTWLLREGWKRAGSAISARTVPSKPR"
BASE COUNT          520 a         1101 c         1038 g          505 t
ORIGIN      
        1 gccgcgatct ccggcggcgc accggctcga cacaagatcc ctgcgatgcc gcacatggaa
       61 gatccgtcct cgccgcgacg gccgcgatcc gccggcagcg gctcgcgccc ttcgcgctgg
      121 cgtcgtgttg cagtcgccat accgcgctgc ggcgaaactc ccgcgcgcgc gacgcatcgt
      181 catcgcgacg gcacaggtga ctgcgcgcac gccgcgtcag cagcgcacca caggctgcgc
      241 cgcgccggac acgcgcggct cgtcgagact cgcccccgcc gcgaccgccc gatcgacgac
      301 cgaccgcgga acggcaccgg acgggccgcc ggcctctcat ggggcaatct gccccagatg
      361 ccgctgcgcc ggccccgcgc cacacctcac gagtcaacgc acgttcacag ccggccggcc
      421 ggcgatccag cgcaaggcca cttgcgcgcc gtccccggcg gttccgccgc gcggccgtgc
      481 tggcatctgc ctcgcagtac ccaggaacat actcacgctg ccggatcttg acggacggcc
      541 acgcagtgtg aggggccacc gaggcatggt gcctcggtgg ctttgctttt ttcggcgcgg
      601 cggcgacgtg cgttcggccg atccgggcgg cggcgcgcaa gatcgtcgca gctcccgccg
      661 gctccggcgt ccggggccgt ggcggcttgg cggcggctgc ggagtgctag tgtcagggcg
      721 atggtttcgc gctggctcgg tgtcgtcctc gtggtctgtg cgtgcggtgg cgcgagcggt
      781 ccggtgtcgc ccgccgccga aggcgtgcgc gcctacgtgc gcgcgctgcg cgacaacaac
      841 ccgcgcgagg cctacgcgct gctgtcggcc gaggcgcgca agaagatcag cttcgacgag
      901 ttcgcgctgg cctggaagca gagcgacaag gagcgcgcct ggcaggcccg ggcgctcgac
      961 gacggcctgc gcggcaaccc cgacgtcggc gagcgcgcgc tgatcagctt ctccgacggc
     1021 aagctggtgc agctcgagcg cgagggcaag acctggcggc tcgagagcga gctggtcagc
     1081 cggtcgcgcg ccaagcagcc gcgcgacgcg atccggctgt tcgccgaggc gatcacggcg
     1141 cgcgacatca ccggcgtgct caacgtgctg acccagcgcc ggcgcgacgg cctgacccgc
     1201 caggtcgagg gcttcgtcgc cggcatcggc aagcggatca acgaccggat cgaccagttc
     1261 ggcaccgacc gcgccgagct gcggtgggac gagaacggga tccgctaccg gatcgtgctg
     1321 cgcaaggagg aggacgagtg gcgggtcgac gacatctaca tccggccggc gcccaaggac
     1381 gacgacggcg acaagagcga gggaagcgtc cccgaggact tctgacccgc cgggcggttt
     1441 gcgtctcacc ttcgctgatt tcgatcacgt gcatcgcagg gaaggatcgg aacctgtcat
     1501 tcgatgattt cggcgatcta ccgggccgtg ctccagggca tggaagcgtc gcccgccgat
     1561 cggctcgacc agctcgcgcg cacctccatc gtcgtgttca gcgcggcttg ctcggcacgg
     1621 tccgggcgct gatcgcactt cctgcgcgct tccagccctc ccggagcaac cacgtctggg
     1681 gcctccgcac catcggtgga tcgtagcctt ctcggatcgg ccacatgcat ggctgcccgt
     1741 ccagctcctg ccagtcggga aacatgatcg cgctcgagaa cggatcgacg agccaggtgc
     1801 tcggcagctc ggcctggtcc tcgccgtgct tccgccagtt tcccaggacg taggaaatcg
     1861 cgtggcgcgc gcgagtcggc gtggtgatga cctccacgtg atagcggtcg gagaatacct
     1921 tgccgcggcg ccgccgcgca ccgtcgccga gcgcggtgtt gatgtgcctc gccgccgaga
     1981 tcaagaagcc ctgcatcccg cgggacagcg cggccttgtg ctcggcctcg acgagcaagt
     2041 gcacgtggtt gcgctgcagc gagatgtgca cgatccggaa ccactcgcgc agcgcagcgg
     2101 tgatggtggc gtcgcgcatc gccttgtaca gcttgcggca ccgcaggctg cccaccgctg
     2161 gcgcgacccg gaccacgaca tgcagcgcgt ggtacggctt gaagtccgga cgcgcctggt
     2221 ggcgctctcc ggcgcgcgct cctctgggct ttcgcccggc accacgccgc ttgccaccgc
     2281 gccggaacag ctcacgctgc cgcggctgct gcttgccacc acgcttcgac tccctctgcg
     2341 ccatgaccca tggatagtca tcgtcgtgca gcatgtcaat tggatgggca ctactaactt
     2401 ctatttattt cattgggctc gatcgccaag tagcattcac ccagaatgca atcgcttcgt
     2461 gacaacagcg tttgatcaga gacagatgtc tccagttctt cgcaacctgc gatcgagctt
     2521 gatgccactc cgaccgatcc acgcgatcat gcaccaccgc gttcggccgg ccgcagagcg
     2581 cacagaccgc tcgctggatc agccagctgc tcgacggcga tcgagacagc gcatcgcccg
     2641 ccgtcgttgc ggatggtccc cgaccgcaag ctctgcagag catgtggacg gtcctgtgga
     2701 caactggaag gtctgatgac acgcgccgca gggcgccgag gtcgacctgg acgcgtctca
     2761 cagcccttct tcagttgccc acagtcctga cgctcgagcc agtctccaat cagagtttaa
     2821 tttttggttt attttggatt cagaacagga gagcgattgt cccaggacgc gaagatgact
     2881 gttcaacaca aggatcaatg aacaatccaa tctgattgac ttcccgtgct gtccggcgca
     2941 tgtagaagcg ttcctccttc gggaacgagg cagcgccgcc gttggcgcga ggcacgactg
     3001 acatagcgga ccgccggtgc tggaacaccg gcgatccaag gagccgacga tgcgttcaca
     3061 tcgcagcacc caggccaggg tatcgaacga cccgcagctc agcaacttcg aaccgggcac
     3121 cgcgctggag aacctccgcg ccgagatgat cgacatcgag gcgc
//
LOCUS       DASVOR010001908         1865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_428360,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001908 DASVOR010000000
VERSION     DASVOR010001908.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428360"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1865
                     /locus_tag="VF516_35410"
     CDS             <1..>1865
                     /locus_tag="VF516_35410"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8113090.1"
                     /translation="KPAVAVGSLRHGEPARRTLLRSLASLYATGVDVAWRRLFAGTAA
                     VALPRYPWQRERYWFARAEAPRTASHGVGPARDASPPDRGDRGDPVRDLVTRELARVL
                     EIADAAAIDPRRGFRELGVTSLMAMELVGRLGSALGRALPSSVVFNHPNVEALASHLA
                     RVGERPATVPRARDDRDRRVTDPIDPTGEAPRPTPRDTASPIDPTGEGPRSAGRAVGG
                     PTPRDTALPIAVVGMSCRFPGASDPHAFWSLLRGGGDAIREVPPERWDLERWYAPEPG
                     RPGKMQTRWGGFLDDIDRFDAAFFGISPREAEEMDPQQRLVLEVAWEALERAGLAPDR
                     LLGTEAGVFLGLMNTNDYAARKRLHDDPARIRAHHGTGIAASIAAGRLAYLLGSTGPA
                     MAIDTACSSSLVAIHQAMRSLRSGECRLALVGGVNAILSPEITVAYSQAGMLSPTGRC
                     RTFDASADGYVRSEGCGMLVLERLSDAVAGGRAVLAVLAGAAVNHDGRSSGLTAPNGS
                     AQCAVIRAALDDAGLTPADVDYVEAHGTGTRLGDPIELHALAEVFGDRPRPLRVGSVK
                     TNVGHLEAAAGVAGLMKVILALQHREIPPHLHLREHTPLLAAGGSTSRGIERSETG"
BASE COUNT          223 a          697 c          707 g          238 t
ORIGIN      
        1 aagccggccg tcgcggtcgg ttcgctgcgg cacggcgagc ccgcgcgccg cacgctcctg
       61 cgctcgctgg cctcgctcta cgccaccggc gtcgacgtcg cgtggcggcg gttgttcgcc
      121 ggcaccgccg ccgtcgcgct acctcgctat ccgtggcagc gcgagcgcta ctggttcgcg
      181 cgcgcggagg cgccccggac ggcatcgcac ggggtcggtc cagcccgcga tgcatcaccg
      241 ccggaccgcg gcgatcgcgg cgatccggtg cgcgacctgg tgacccgaga gctggcccgc
      301 gtgctcgaga tcgcggacgc cgccgcgatc gatccgcgcc gcggcttccg ggagctcggc
      361 gtgacgtccc tgatggcgat ggagctggtc ggccggctcg ggtccgcgct cggccgcgcg
      421 ctgccatcgt cggtggtgtt caatcatccc aacgtcgagg cgctggcgag ccacctggcg
      481 cgggtgggcg agcgtccggc gacggtcccc cgcgcgcgcg acgaccgcga tcgccgcgtc
      541 accgacccga tcgatcccac gggggaggcc ccccgcccca ccccccgcga tacggcttcg
      601 ccgatcgatc ccacggggga gggcccccgc tccgccggca gagccgtcgg gggccccacc
      661 ccccgcgata cggccttgcc gatcgccgtc gtcgggatga gctgccggtt ccccggcgcg
      721 tccgatccgc acgcgttctg gagcctgctg cgcggtggcg gcgatgcgat ccgcgaggtg
      781 ccgcccgagc gctgggacct cgagcgctgg tacgccccgg agccgggccg cccggggaag
      841 atgcagaccc ggtggggcgg gttcctcgac gacatcgacc ggttcgatgc ggcgttcttc
      901 ggcatctcgc cgcgcgaggc cgaggagatg gacccgcagc agcgcctggt gctggaggtc
      961 gcgtgggagg cgctcgagcg cgccgggctg gcgccggacc ggctgctcgg gaccgaggcc
     1021 ggcgtgttcc tcggcctgat gaacaccaac gactacgcgg cgcgcaagcg gctgcacgac
     1081 gatccggcgc ggatccgcgc gcaccatggc accggcatcg cggccagcat cgccgccggg
     1141 cggctggcct acctgctcgg ctcgacgggg ccggccatgg cgatcgacac cgcgtgctcg
     1201 tcgtcgctgg tcgccatcca ccaggcgatg cggagcctgc gcagcggcga gtgcaggctg
     1261 gcgctggtgg gcggggtcaa cgcgatcctg tcgcccgaga tcacggtggc ctactcgcag
     1321 gccgggatgc tgtcgcccac cgggcggtgc aggacgttcg acgcctccgc cgatggatac
     1381 gtccgcagcg agggctgcgg gatgctcgtg ctcgagcggc tgtcggacgc ggtcgccggt
     1441 gggcgcgccg tgctcgcggt cctggccggc gcggcggtga accacgacgg ccggagcagc
     1501 gggctcaccg cacccaacgg cagcgcgcag tgcgccgtga tccgggccgc gctcgacgac
     1561 gccggcctga cgccggcgga cgtggactac gtcgaggccc acgggaccgg gacgcgcctg
     1621 ggggacccga tcgagctcca cgcgctggcc gaggtgttcg gagaccgtcc ccggccgctc
     1681 cgcgtggggt cggtcaagac caacgtcggt cacctcgagg cggcggcggg agtcgccggg
     1741 ctcatgaagg tgatcctcgc cctgcagcac cgcgagatcc caccgcacct gcacctccgc
     1801 gagcacaccc cgctgctcgc ggcggggggc agcacctccc gtgggatcga gcgcagcgag
     1861 acggg
//
LOCUS       DASVOR010001909         1990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_487712,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001909 DASVOR010000000
VERSION     DASVOR010001909.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487712"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..783
                     /locus_tag="VF516_35415"
     CDS             145..783
                     /locus_tag="VF516_35415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113091.1"
                     /translation="MTTIQTHSKSINPSVTGKRRSVLAAAMLVALGGLALGTSAPAMA
                     HPKKKAHEEKKEDRKEGKEEKGGWHTAQLTTYTSYPRCCKGSPAYDPHASKEECEDYS
                     GCKYQGEFAAIGKKSFEWVKDNAVVAFYDNADKQGREFNKKYGGKKIRLRKNGKEFTA
                     VIADTCGNSDCDDCCIKNSKGGFLVDMEFWTAQRNIGGANKAEGTIEFQILD"
     gene            878..1507
                     /locus_tag="VF516_35420"
     CDS             878..1507
                     /locus_tag="VF516_35420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113092.1"
                     /translation="MLRTTLLSILTFTLCLTGRLSTAAAQVGTGWTEIPRDEIRETPR
                     DRVKQVTDDVRTAGSASLEGVEALQLVSCVFHRVERRYLDEYHRGTRQLQADVTLAQP
                     TDAPSPLSGALRGGLQLVKTVADAGSAVLGDRGGILAALSGRPFRLNLIHDADAGELH
                     VYINGLAVWTGRSPATTFFARRGCYGPLQAASARIQLRHVKLFSGGDGE"
     gene            complement(1513..>1990)
                     /locus_tag="VF516_35425"
     CDS             complement(1513..>1990)
                     /locus_tag="VF516_35425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8113093.1"
                     /translation="FSPDGKRIASASDDKTVRVWSADGRGAPVVLRGHGDAVAWVEFS
                     PDGGRIVSASKDGTIRLWPADGGGEPVILTGHECGVAQVRFSPDGRRIASTGSDHTVR
                     LWRELGPASLDDLRLWQATSYCLPVERRVDLLAASPEQARLDRQRCAERVARALAQ"
BASE COUNT          334 a          719 c          691 g          246 t
ORIGIN      
        1 gccgcgatcc tgcggacttg ctcgcgggat cgcgcgcgac cgcgctggcc ccgaccttgc
       61 ctaagggtct gtccgcgcgg cgatgacgcc cgcagcgacg gaccccgagc atcaccagtt
      121 cttcgacgag aaagggatac gaccatgacc accatccaga cccacagcaa gtcgatcaac
      181 ccctctgtca ccggcaagcg gaggagcgtg ctcgcggccg cgatgctggt cgcgctcggc
      241 ggcctcgcgc tcgggaccag cgcgccggcg atggcgcacc ccaagaagaa ggcgcacgag
      301 gagaagaagg aagacaggaa ggagggcaag gaggagaagg gcggctggca caccgcgcag
      361 ctcacgacct acacgtcgta cccgcggtgc tgcaagggct cgccggccta cgatccgcac
      421 gcctccaagg aggagtgcga ggactacagc ggctgcaagt accagggcga gttcgccgcg
      481 atcggcaaga agtcgttcga gtgggtcaag gacaacgcgg tcgtcgcgtt ctacgacaac
      541 gccgacaagc agggcaggga gttcaacaag aagtacggcg gcaagaagat ccggctgcgc
      601 aagaacggca aggagttcac cgccgtcatc gccgacacct gcggcaactc cgactgcgac
      661 gactgctgca tcaagaactc caagggcggc ttcctggtcg acatggagtt ctggaccgcg
      721 cagcgcaaca tcggcggcgc gaacaaggcc gaggggacga tcgagttcca gatcctcgac
      781 tagcaccggc ggaccgggat ccgcgcgcgg gccccccaat ggtcggcgtg tggatccccc
      841 ccacgcattt tccacttcct gcgacgaggc aaagacaatg ttgcgtacca ccctcctgtc
      901 cattctgacc ttcaccctct gcctgaccgg gcggctatcc accgcggcgg cccaggtcgg
      961 caccggctgg accgagatcc cgcgcgacga gatccgcgag acgccgcgcg accgggtcaa
     1021 gcaggtcacc gacgacgtcc gcaccgcggg cagcgccagc ctcgagggcg tcgaggcgct
     1081 gcagctcgtg agctgcgtgt tccaccgggt cgagcggcgc tacctcgacg agtaccaccg
     1141 cggcacgcgc cagctccagg ccgacgtgac gctcgcccag ccaaccgacg cgccgtcgcc
     1201 gctgtccggc gcgctgcgcg gcggcctgca gctcgtgaag acggtggccg acgccggcag
     1261 cgcggtgctc ggcgaccgcg gcggcatcct cgcggcgctg tccggccggc cgttccggct
     1321 caacctgatc cacgacgccg acgccggcga gctgcacgtc tacatcaacg gcctggcggt
     1381 gtggaccggc cggagcccgg cgacgacgtt cttcgcccgc cgcggctgct acggcccgct
     1441 gcaagcggcg tcggcgcgga tccagctccg ccacgtcaag ctgttctcgg gcggggacgg
     1501 cgagtgatcg gctcactgcg ccagcgcccg cgccacccgc tcggcgcagc gctggcgatc
     1561 gaggcgggct tgctcgggcg atgcggcgag cagatcgacg cggcgctcga ccggcaggca
     1621 gtagctggtc gcctgccaca gccggaggtc gtcgaggctg gccgggccga gctcgcgcca
     1681 cagccgcacg gtgtgatcgc tcccggtcga cgcgatgcgc cggccgtcgg ggctgaaccg
     1741 gacctgcgcg accccgcact cgtggccggt caggatgacg ggctcgccgc cgccgtcggc
     1801 cggccacagc cggatcgtgc cgtccttcga ggccgagacg atccggccgc cgtcggggga
     1861 gaactcgacc cacgcgacgg cgtcgccgtg gccgcgcagc acgaccggcg cgccgcggcc
     1921 gtcggcgctc cacacccgca cggtcttgtc gtcggacgcc gacgcgatgc gcttgccgtc
     1981 gggcgagaac
//
LOCUS       DASVOR010001910         2220 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_21104,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001910 DASVOR010000000
VERSION     DASVOR010001910.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21104"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1594
                     /locus_tag="VF516_35430"
     CDS             <1..1594
                     /locus_tag="VF516_35430"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEX8113094.1"
                     /translation="AERQSFIKLGEGDYQLPSIQLLNYDAATANQIDKTAMLELSAKL
                     TQTLENYGVKGDVVAIRPGPVVTMYEFAPAPGTRVNKIVNLTDDLALALEALRVRIVA
                     PIPGKAAVGIEVPNKTREKVFLKEILADDVFIKGRARLPLALGKDIEGGPSVVDLARM
                     PHLLVAGTTGSGKSVAVNAMISSLLYHCSPEDVRLIMVDPKMLELSIYEGIPHLLLPV
                     VTDPKKANLALRWAVEEMDRRYDLLAQMGVRDIATYNDKVGKQLAKLEADKLRAVAEA
                     AARAVEDDIGKAVAADTPAAADAREQIVTAPPHRLPYIVVVIDEFADLMMCAPKEVET
                     SVARIAQKARASGIHLILATQRPSVDVITGLIKANFPSRIAFSVASKIDSRTILDQTG
                     AEALLGAGDMLFSDRGAAPQRLHGCYVDEEEIHRVVAFLKTQGRPVYNLDILRPREDD
                     GDGQGELGLAGGPGGKSDDDMYDKAVYIVTTTRNASISWVQRQLRIGYNRAARLVEEM
                     EKQGVVSPPDHTNKREVLVAQA"
     gene            1670..>2220
                     /locus_tag="VF516_35435"
     CDS             1670..>2220
                     /locus_tag="VF516_35435"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8113095.1"
                     /translation="MDSDPSNAMDRLAIRELIENWVVWRDAGDWERFATVWHDDGVMM
                     ATWFQGPAAEFIRVTKEGWARGVSILHFLGGSSIEVAGDRAIAQTKMTISQRGDVHGV
                     PCDVVCTGRFYDFLEHRQRRWGLVLRQPIYEKDRIDPVDPAARLTLDADHLAALPRGY
                     RHLAYIQLQIGYAVKRDMPQLTGP"
BASE COUNT          371 a          813 c          743 g          293 t
ORIGIN      
        1 ggccgagcgc cagagcttca tcaagctcgg cgagggcgac taccagctgc cgtcgatcca
       61 gctgctcaac tacgacgccg cgaccgccaa ccagatcgac aagaccgcga tgctcgagct
      121 gtcggccaag ctgacccaga cgctcgagaa ctacggcgtc aagggcgacg tggtcgcgat
      181 ccgccccggc ccggtggtca cgatgtacga gttcgcgccc gcgcccggca cgcgggtcaa
      241 caagatcgtc aacctgaccg acgacctcgc gctcgcgctc gaggcgctgc gggtccggat
      301 cgtcgcgccg atccccggca aggccgcggt cggcatcgag gtccccaaca agacccgcga
      361 gaaggtgttc ctcaaggaga tcctcgccga cgacgtgttc atcaagggcc gcgcccggct
      421 gccgctcgcg ctcggcaagg acatcgaggg cgggccgtcg gtcgtcgacc tcgcgcgcat
      481 gccgcacctg ctggtcgccg gcaccaccgg gtcgggcaag tcggtcgcgg tcaacgcgat
      541 gatctcgagc ctgctctacc actgcagccc cgaggacgtc cggctgatca tggtcgaccc
      601 caagatgctc gagctctcga tctacgaggg catcccgcat ctgctgctgc cggtggtcac
      661 cgatcccaag aaggccaacc tcgcgctgcg ctgggcggtc gaggagatgg accggcgcta
      721 cgacctgctg gcccagatgg gcgtgcgcga catcgcgacc tacaacgaca aggtcggcaa
      781 gcagctcgcc aagctcgagg ccgacaagct gcgcgccgtc gccgaggccg cggcccgcgc
      841 ggtcgaggac gacatcggca aggcggtcgc cgccgacacc ccggccgccg ccgacgcgcg
      901 cgagcagatc gtgaccgcgc cgccccaccg cctgccgtac atcgtggtcg tgatcgacga
      961 gttcgccgac ctcatgatgt gcgcgcccaa ggaggtcgag accagcgtcg cgcggatcgc
     1021 gcagaaggcc cgcgcgtcgg gcatccacct catcctcgcg acccagcgcc cgtcggtcga
     1081 cgtcatcacc ggcctgatca aggccaactt cccgtcgcgg atcgcgttct cggtcgcctc
     1141 gaagatcgac tcgcgcacga tcctcgacca gaccggcgcc gaggcgctgc tcggggccgg
     1201 cgacatgctg ttctccgacc gcggcgccgc gccccagcgc ctgcacggct gctacgtcga
     1261 cgaggaggag atccaccgcg tcgtcgcgtt cctcaagacc cagggccggc cggtctacaa
     1321 cctcgacatc ctcaggccgc gcgaggacga cggcgacggc cagggcgagc tcggcctggc
     1381 cggcggcccg ggcggcaagt ccgacgacga catgtacgac aaggcggtct acatcgtcac
     1441 caccacgcgc aacgcgtcga tctcgtgggt ccagcgccag ctgcggatcg gctacaaccg
     1501 cgcggcgcgg ctcgtcgagg agatggagaa gcagggcgtg gtcagcccgc cggaccacac
     1561 caacaagcgc gaggtcctgg tcgcccaggc ctgaggttcg gcgaggttca gcgaggttca
     1621 gcggcgcccg ccggcatggc atgctcgccg ccacggaaag gcggccgcga tggacagcga
     1681 cccgagcaac gcaatggatc gcctcgcgat ccgcgagctg atcgagaact gggtcgtgtg
     1741 gcgcgacgcc ggcgactggg agcggttcgc cacggtctgg cacgacgacg gcgtgatgat
     1801 ggcgacctgg ttccagggcc cggcggccga gttcatccgc gtcaccaagg aaggctgggc
     1861 gcgcggcgtg agcatcctgc acttcctcgg cggcagctcg atcgaggtcg ccggtgaccg
     1921 cgcgatcgcg cagaccaaga tgacgatctc gcagcgcggc gacgtccacg gcgtgccgtg
     1981 cgacgtcgtc tgcaccggcc ggttctacga cttcctcgag caccgccagc ggcgctgggg
     2041 cctcgtcctg cgccagccga tctacgagaa ggaccggatc gatccggtcg acccggccgc
     2101 gcgcctgacg ctcgatgctg accacctcgc ggcgctgccg cgcggctacc gccacctcgc
     2161 gtacatccag ctgcagatcg gctacgccgt caagcgcgac atgccgcagc tcaccggccc
//
LOCUS       DASVOR010001911         1623 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_487727,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001911 DASVOR010000000
VERSION     DASVOR010001911.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487727"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="VF516_35440"
     CDS             complement(<1..1289)
                     /locus_tag="VF516_35440"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113096.1"
                     /translation="MTRYELLDRIGVGGMAEIFRGRAVAAGGFEKPVAIKRILPHLSQ
                     DKRFVELLIAEAKVLSLLKHRNIVQIFDVGLGDDDQYFLVMEYVDGKDLGAVQRGLEA
                     RRKRIPFDLALHVGAEICEALEHAHSARAPDGKPMSLVHRDVSPSNVLLSRAGEVKLT
                     DFGIAKRAEQESTGHGAVRGKFAYISPEQARNEHIEPRSDVFSVGILLWELVTSRRLF
                     SGLGDMEALRAVREAQVPRPSGFDGALSPEIDALIMSALSPEPRVRPTAGQLGARLRS
                     LRYALDVTVGDPATELAKFIDTAEDHERESAQRLPSARAEFEGSEATVIRIHTADAFS
                     MRDKDGAGVARAREVIDRFEEEETRQAQLSRDQMAFLRAHAQRDSGELSQAVEPPLPG
                     VSPRRTSDELTRAHVPMSTDDVPTLYPGLGPGLGPGLG"
     gene            1366..>1623
                     /locus_tag="VF516_35445"
     CDS             1366..>1623
                     /locus_tag="VF516_35445"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8113097.1"
                     /translation="MHEIAALLDTALATSLGSAAALSIGDAGVEQERLVRGRVHRVPD
                     LGPAIDEHTAFDLASLTKPMATVGLAMVLAGDGALDLDAPVR"
BASE COUNT          207 a          627 c          567 g          222 t
ORIGIN      
        1 ccgaggcccg gcccgaggcc tggcccgagg cctggataga gcgtcggcac gtcgtcggtg
       61 gacatcggga cgtgggcgcg ggtcagctcg tcgctggtcc gccgcggcga cacgcccggc
      121 agcggcggct cgaccgcctg ggacagctcg ccggagtcgc gctgggcgtg cgcgcgcagg
      181 aacgccatct ggtcgcgcga cagctgggcc tgccgggtct cctcctcctc gaagcgatcg
      241 atcacctcgc gcgcccgcgc caccccggcg ccgtccttgt cgcgcatcga gaacgcgtcg
      301 gcggtgtgga tccggatcac cgtggcctcg ctgccctcga actcggcgcg cgccgacggc
      361 agccgctgcg cgctctcgcg ctcgtgatcc tcggcggtgt cgatgaactt cgcgagctcg
      421 gtcgccggat cgccgaccgt gacgtcgagc gcgtagcgca ggctgcgcag cctggcgccg
      481 agctgccccg cggtcggccg cacgcgcggc tcgggcgaca gcgccgacat gatcagcgcg
      541 tcgatctccg gcgacagcgc gccgtcgaat ccgctcggcc gcggcacctg ggcctcgcgc
      601 accgcgcgca gcgcctccat gtcgccgagc cccgagaaca gccggcggct ggtcaccagc
      661 tcccacagca ggatcccgac ggagaacacg tcgctgcgcg gctcgatgtg ctcgttgcgc
      721 gcctgctccg gcgagatgta cgcgaacttg ccgcgcaccg cgccgtggcc ggtcgactcc
      781 tgctcggcgc gcttggcgat cccgaagtcg gtcagcttga cctcacccgc gcgcgacagc
      841 agcacgttcg acggcgacac gtcgcggtgc accagcgaca tcggcttgcc gtcgggcgcg
      901 cgcgccgagt gcgcgtgctc gagcgcctcg cagatctccg cgccgacgtg gagcgcgaga
      961 tcgaacggga tgcgcttgcg gcgggcctcg agcccgcgct gcaccgcgcc gaggtccttg
     1021 ccgtcgacgt actccatcac caggaagtac tggtcgtcgt cgccgagccc gacgtcgaag
     1081 atctgcacga tgttgcggtg cttgagcagc gacagcacct tggcctcggc gatcagcagc
     1141 tcgacgaacc gcttgtcctg cgagaggtgc ggcaggatgc gcttgatcgc gaccggcttc
     1201 tcgaacccgc cggccgccac ggcccggccg cggaagatct cggccatccc gccgacgccg
     1261 atccggtcga gcaactcgta ccgcgtcacg aagcgcgcat cctagcacgc ccgccgggcc
     1321 gccgccctcc ggcgccgccc gccgccggac gtgtacccta cgcccgtgca cgagatcgct
     1381 gccctgctcg acaccgccct cgccacttcg ctcggctcgg cggcggcgct gtcgatcggc
     1441 gacgccggcg tcgagcagga gcggctggtg cgcggccggg tccaccgcgt ccccgacctc
     1501 ggcccggcga tcgacgagca caccgcgttc gacctcgcgt cgctgaccaa gccgatggcg
     1561 accgtggggc tcgcgatggt gctggccggc gacggcgcgc tcgacctcga cgcgccggtc
     1621 cgg
//
LOCUS       DASVOR010001912         3110 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_546242,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001912 DASVOR010000000
VERSION     DASVOR010001912.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546242"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..479)
                     /locus_tag="VF516_35450"
     CDS             complement(165..479)
                     /locus_tag="VF516_35450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113098.1"
                     /translation="MSFRTISLGLLAALALGCGGDPPRSDDPQTAKEKQRREAAAAGE
                     TSSAGGKWGGWRYTGDREECFFVVGRKCFKTEAAACAAARCGSRKCQTTGGGPAAVAC
                     AK"
     gene            508..1176
                     /locus_tag="VF516_35455"
     CDS             508..1176
                     /locus_tag="VF516_35455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113099.1"
                     /translation="MADDDRALRERLAALEAEVKADADAQRARKAAALAKLRDQRVTE
                     TAGGSLVVRNRATPGDDAAARRRSRLASRDPEPEIDDGRGRADPLEDLSGALELASKA
                     NRVKQELARKPRKGEKNWMISGGASLLGPIGWLYAGSLREAVPATAAWLLVASVVWKI
                     LPFLWMPLMMVVMPLSAIAGVIYALSYNRAGGRQRLFSDKKDKALPPGSARAHPRSKL
                     GDDS"
     gene            1264..2100
                     /locus_tag="VF516_35460"
     CDS             1264..2100
                     /locus_tag="VF516_35460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113100.1"
                     /translation="MRSVVLVVALALLPRPASADRTRAKVAVVPGIVVNLAAARVDAL
                     TQELAEALQAELEVEAIGGVEVRRRLPPAGLPSDCVASEPCIADVARRLDADQLLFVV
                     MIDTGSSGAIQIDSTWVDPVAHRSGSRPAIDIATIADARPRFAAAASQLLPDAPVRPK
                     PQAAGLGRMSEPVPRHFTLPAYLTTGATVVGLGVGLSLGLDARGRYNDCTAQAHDGTA
                     CSQSRKDAIRRIALAADAGWLVAIGGTIATAILYATSGEASHVIVEPVPGGAAVTASG
                     SF"
     gene            2100..>3110
                     /locus_tag="VF516_35465"
     CDS             2100..>3110
                     /locus_tag="VF516_35465"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113101.1"
                     /translation="MSAGLAPSDKLLGQILADRYQILHRLGEGAMGVVYKARHVKVGR
                     LFAVKLLHPRVLQDAKVVRRFEREAELAGRLRHPNVIGVVDVGEVDGTRYMVMDFAEG
                     PDLAKLLDEAPMPPERIIRLVRPMLEGLYHAHEQNLIHRDFKPENVIVERDSHGAEVP
                     RIVDFGIALLREGGDSFDGTGRLTTNGLVLGTPHYMAPEQAVADPIDHRIDLFALGIV
                     IYEMLSGRLPFDGSGAEVARANLLLDPPPIHQRVEHLEVDPLLEAFARRLMAKKRTAR
                     PATAKAARELLDLIEHDRAAAAVQLGVPVARSGRSAATESVTVVDDPATAVDVDGATV
                     RMQ"
BASE COUNT          434 a         1096 c         1162 g          418 t
ORIGIN      
        1 ggccgtgccg ggagatgacg atggaagccg caggcgacga cctgctggcg ccgatcatcg
       61 acggcgagtt ctaccgcaac gtcaagcgcc tcgcgttcca cgtcgggccg cgggtccgga
      121 tccccaaggt ggtcgcgccc aaggtagcgt aggcggtcgg ccgatcactt ggcgcaggcc
      181 accgcggcgg ggccgccgcc ggtggtctgg cacttgcggc tgccgcagcg cgcggccgcg
      241 caggccgccg cctcggtctt gaagcacttg cgcccgacga cgaagaagca ctcctcgcga
      301 tcgccggtgt agcgccagcc gccccacttg ccgccggcgg acgaggtctc gccggccgcc
      361 gcggcctcgc ggcgctgctt ctccttggcg gtctggggat cgtcggacct cggcggatcg
      421 ccgccgcagc cgagcgcgag cgcggcgagc aaaccgagcg agatcgttcg gaacgacatc
      481 gcggcagatc atgccatagt cgaagccgtg gctgacgacg accgcgcact gagggagcga
      541 ctggccgcgc tggaggccga ggtcaaggcc gatgcggacg cgcagcgcgc gcgcaaggca
      601 gcggcgctcg ccaagttgcg cgatcaacgt gtgaccgaga ccgcaggcgg ctcgctggtt
      661 gtgcgaaacc gcgcaacgcc gggcgacgac gccgcggcgc gacgccggtc acggctggcg
      721 tcgcgcgacc cggagccgga gatcgacgac ggccgcggcc gcgccgatcc gctcgaggac
      781 ctcagcgggg cgctcgagct cgcgtcgaag gccaaccggg tcaagcagga gctggcgcgc
      841 aagccgcgca agggcgagaa gaactggatg atctccggcg gggcctcgct gctggggccg
      901 atcggctggc tgtacgcggg ctcgctgcgc gaggcggtgc cggcgacggc cgcgtggctg
      961 ctggtcgcgt cggtggtctg gaagatcctg ccgttcctgt ggatgccgct gatgatggtg
     1021 gtcatgcccc tgtcggcgat cgccggcgtg atctacgccc tttcgtacaa ccgcgccggc
     1081 gggcggcagc ggctgttcag cgacaagaag gacaaggcgc tgccgccggg gagcgcacgc
     1141 gcacaccctc ggagcaagct aggcgacgac tcgtgacagc gacggcggat taacgcgttt
     1201 ctaaccgccg gcgcggcgta tcatccggct gcgctcgacg ggagaatcgt tatgcttcct
     1261 aggatgcgtt ctgtggtcct ggttgttgcg ctcgccctgc tgccacggcc ggcgagcgcg
     1321 gatcgaacgc gcgcaaaggt cgccgtggtc ccggggatcg tggtcaacct cgccgcggcg
     1381 cgggtcgacg cgctgaccca ggagctggcc gaggcgctgc aggccgagct cgaggtcgag
     1441 gcgatcggcg gcgtcgaggt ccggcgccgg ctgccgcccg ccgggctgcc gtcggactgc
     1501 gtcgccagcg agccgtgcat cgccgacgtc gcgcggcggc tcgacgccga tcagctgctg
     1561 ttcgtggtca tgatcgacac cggctcgagc ggcgcgatcc agatcgacag cacgtgggtc
     1621 gacccggtcg cgcaccgcag cggctcgcgc ccggcgatcg acatcgcgac gatcgccgat
     1681 gcccggccgc ggttcgccgc tgcggcatcg cagctcttgc ccgacgcgcc ggtccggccc
     1741 aagccgcagg cggccggtct ggggcggatg tcggagccgg tgccgcggca cttcacgctg
     1801 ccggcgtacc tcacgaccgg cgccacggtc gtcggcctgg gggtcggtct ctcgctcggc
     1861 ctcgatgcgc gtggccggta caacgactgc accgcgcagg cgcacgacgg cacggcgtgc
     1921 tcgcagagcc gcaaggacgc gatccgccgg atcgcgctgg ccgccgacgc cggctggctg
     1981 gtcgcgatcg gcgggacgat cgccaccgcg atcctgtacg cgacgtcggg ggaggcctcg
     2041 cacgtgatcg tcgagccggt cccgggcggc gcggcggtca ccgcgtccgg gagcttctga
     2101 tgagcgccgg gctggcgccg agcgacaagc tgctcggcca gatcctcgcc gaccgctacc
     2161 agatcctgca ccgcctcggc gagggcgcga tgggcgtggt ctacaaggcg cgccacgtca
     2221 aggtcggccg gctgttcgcg gtcaagctgc tgcacccgcg cgtgctgcag gacgccaagg
     2281 tcgtgcgccg gttcgagcgc gaggccgagc tcgccggccg gctgcgccac cccaacgtga
     2341 tcggcgtggt cgacgtcggc gaggtcgacg gcacgcgcta catggtgatg gacttcgccg
     2401 agggccccga cctcgcgaag ctgctcgacg aggcgccgat gccgcccgag cgcatcatcc
     2461 ggctggtccg cccgatgctc gagggcctgt accacgcgca cgagcagaac ctgatccacc
     2521 gcgacttcaa gcccgagaac gtgatcgtcg agcgcgacag ccatggcgcc gaggtcccgc
     2581 gcatcgtcga cttcgggatc gcgctgttgc gcgagggcgg cgactcgttc gacggcacgg
     2641 gccggctgac caccaacggc ctggtgctcg gcacgccgca ttacatggcg cccgagcagg
     2701 cggtcgccga cccgatcgac caccggatcg acctgttcgc gctcggcatc gtgatctacg
     2761 agatgctgtc gggccggctg ccgttcgacg gcagcggcgc cgaggtcgcg cgagccaacc
     2821 tgctgctcga tccgccgccg atccaccagc gcgtcgagca cctcgaggtc gatccgctgc
     2881 tcgaggcgtt cgcgcgccgc ctgatggcca agaagcgcac cgcgcggccg gcgaccgcga
     2941 aggccgcgcg cgagctgctc gatctgatcg agcacgatcg cgcggcggcg gccgtgcagc
     3001 tcggcgtgcc ggtcgcgcgg agcggccgct cggcggcgac cgagtcggtg accgtcgtgg
     3061 acgatccggc caccgcggtg gacgtcgatg gcgcgacggt gcggatgcag
//
LOCUS       DASVOR010001913         6203 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_546245,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001913 DASVOR010000000
VERSION     DASVOR010001913.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6203
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546245"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VF516_35470"
     CDS             complement(<1..210)
                     /locus_tag="VF516_35470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113102.1"
                     /translation="MSSTNWTEVVVPVGAADVDDVAALIASEVVAAAAGTEQRGDEVV
                     FWVAADAAAEALAEVRDAAQRWQAGG"
     gene            complement(241..468)
                     /gene="rpsU"
                     /locus_tag="VF516_35475"
     CDS             complement(241..468)
                     /gene="rpsU"
                     /locus_tag="VF516_35475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEX8113103.1"
                     /translation="MENSFGKPVEVEVRDSLEKAMKILKQKMSKEGILQELKRRRFYE
                     KPSVKRKRKTREARKRLRREMKRRIVPAAPR"
     gene            complement(693..887)
                     /locus_tag="VF516_35480"
     CDS             complement(693..887)
                     /locus_tag="VF516_35480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002640100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113104.1"
                     /translation="MIDVTGVKVFSATKAKERELLGELITDWIRSHPDHEIVDKIVTQ
                     SSDSEFHCLTITLFYKHNKS"
     gene            1160..2524
                     /locus_tag="VF516_35485"
     CDS             1160..2524
                     /locus_tag="VF516_35485"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8113105.1"
                     /translation="MRRSRFVKHAVILAGVASAIPAPAAPEPPKIVGKLPALETFSLS
                     NGLQVAVLRTDAAPVVSVQLWYHVGSKDEPRERRGTAHMFEHLMFEGTARVRPQLHAQ
                     SIEWLGGYVNASTDEDATHYIDTLPPEHLDYALQLEADRMRGLRLTKPAIDAGRQLIQ
                     AELRQQDASPFAQGLIRCLAFAYQKHPYASMSTGNAKELDAVTADELKKFYDAYYQPN
                     NALLVVVGKVTPADVKASVEKLFGPIARGADPPRPADATPEPAQTARRHQVVDPGAVG
                     LTLVGWHVPPARDKDIFPLQLALNVLGTGDGSRLKARLKAPDPKTKQPLALDAAMDAF
                     IREQPGMVIALGGYLDPAKADAVEAALVDEVGKLAAKGPTADELRKAKNQVEAGFVFS
                     LENAQGLGEAIGRSWIFTGNPGSFLYEADELDKVSAVDVQRVVKQYLSPDRATVVVIP
                     PKAR"
     gene            2527..4842
                     /locus_tag="VF516_35490"
     CDS             2527..4842
                     /locus_tag="VF516_35490"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8113106.1"
                     /translation="MRTLLVTTALGAAACTLGAACGPKPASAPIPMLPGDGDAHVVKP
                     PPPRPPPPPDPYAGRSDLIAVPSVRPPGPVELPKLEEWKLGNGLQVYAVRSPRLPVVA
                     MQLAVRAGRMNEPRSRLGVSELTADMLVKGTRTRDAAALAKAIDFVGGTISADSTFEA
                     TILSCSVLARSAGTCFDLVSDMVTQPAFPDGELTALRDSLVAKIQQRTEDPAALASAH
                     AQNLLWGNDHIRGWINSAQSMGSIRRDDIAAWHRAWFVPGNAMLVVVGDIDPKRLRGD
                     LERTFGAWRKGPVPPVPAYPEQGLSGTRIRLVDKPGLAQTQIRIAQFGIKHDDARFFD
                     TLVWNFALGGGQGSRLARAVRGDGSKGYVAGSGFDRNSDRGSFVASSVTRNADAVATT
                     RQMIAEIAKMAKDGPTQDEVGTAIVSITGGYGLRFQAAADFGGALVTAELHGFGQEYL
                     TNYPVAVGQVSVAEAKRAAAEILDPKAYVVVLVGDAKDLEPALHKEGWRYEKIAYTDP
                     VSPPYVAPEAPADAKAIAAARKLIDDALVAKGGKAKLAGLKGLKLVATGVSTFSGQPV
                     PVEIARTLAVPDKMRIDATLKLPQPQGDAVVSIVVAGKAGWQRAPDGRGGYALVDITG
                     PALATADFERWREPELILLKAAEPDAKLTPMADETVDGKPCSVVKLRSPLPGVDVELY
                     IDKKTRMVSRMIYSDGGSTETDEFSDYRDASGLKVAYRRVTSGAGRSTALDIKSVELD
                     PKIEPTLFDKPAPSDKPAQSDKPAQSDKPAK"
     gene            4839..6038
                     /locus_tag="VF516_35495"
     CDS             4839..6038
                     /locus_tag="VF516_35495"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX8113107.1"
                     /translation="MTARVGSVAALAAVLAAAVAVVARAQPAHLASGEVPAGAVEPEP
                     PAIELLTFGVGARIFEKFGHAAICLRYHQPEHPAICFNYGVTNFSAGAVMVWSFLRGQ
                     QKFWVEPTTLDAMLAFYRGEDRDIWRQTLPITGDAARAIEARLWSDIREDHRYYMYDH
                     FYDNCSTRLRDMIDQATGGALSASRATRHPRTFRELGRRGLAGMPLLLVTSDLIIGRE
                     LDQHPTMWQAMFYPEILRSVGARQLHAPPELIYHRRGPPFPIRGNSGRLHFLAVAVAL
                     AVPLFIAHAVRRHQAAALWAVSVALALLGVIVWTVAIASSIPAVRYNEAVFVVMPLDL
                     ALPLLSPDWRRRYARGRIAVLAVFLALALVGVFRQPLWIVVLAVALPMATVAFDLPHG
                     RQRPETA"
BASE COUNT          927 a         2219 c         2149 g          908 t
ORIGIN      
        1 gccgccggcc tgccagcgct gtgctgcatc gcgaacctcg gccagcgcct cggcggcggc
       61 gtccgcggcg acccagaaca ccacctcgtc gccgcgctgc tcggtgccgg ccgcggcggc
      121 gacgacttcg gaagcgatca gcgccgcgac gtcatcgaca tcggcggcgc cgacggggac
      181 cacgacttcg gtccagttcg tggaggacac gttcgtcgac agattgcgaa gctcgacagc
      241 tcagcgcggc gcggccggga cgatccgccg cttcatctcc cggcgcagac gcttgcgcgc
      301 ctcgcgggtc ttgcgcttgc gcttgacact tggcttttcg tagaagcgcc ggcgcttcag
      361 ctcctgcagg atgccttcct tggacatctt ctgcttgagg atcttcatcg ccttctcgag
      421 gctgtcgcga acctcgactt cgacgggctt tccgaacgag ttctccatgg gcgcgaaggg
      481 gtaccctttt aaagaggcgc ggtcaaggag gcgaagggaa gcatgctcga ggacgaaacg
      541 taccggtacc cgcgacaggg catgcgacct cggcgcatcg gcgagacgcg gctcacaaca
      601 gagccgtcgg ctggcaacag agccgtcggc tggcaaccac ccgacggcaa gcgccgcgcc
      661 ctgctgggcg gcagcggcag atgtggcggc cgctacgact tgttgtgctt gtagaacagc
      721 gtgatcgtca ggcagtggaa ctcggagtcc gagctctggg tgacgatctt gtccacgatc
      781 tcgtggtcgg ggtggctgcg gatccagtcg gtgatgagct caccgagcaa ctcgcgctcc
      841 ttggccttcg tcgctgagaa caccttcacg ccggtcacgt cgatcatccg gccttcatcc
      901 accgaagcac ttgctaaaga acgagccaca gcaccctccg tctgagatgc gcccattgtg
      961 ggccttgttg ggtcccacca ctctggcgct aaccatggtt tcagtcaatc ggtactattc
     1021 cgataatgtt gaactaccgg ggcagcgagt gtcaacgtgg tccgcgcagg gaccgttcct
     1081 gataagtgcc tggagccgtg gccgacccac atgcaaccct cgtcggccct ggtagtgtcc
     1141 gcgcgttgga ggagaaggca tgcgtcgatc gcgcttcgtc aagcacgccg tcattctagc
     1201 cggagttgcg tccgcaattc ctgcacctgc ggcaccggaa ccgccaaaaa tcgtcgggaa
     1261 gctcccggcc ctggagacgt tcagcctatc aaacggcctt caggtagcag tgctgcgtac
     1321 cgatgcagcc ccggtggtat cggtgcagct ctggtatcac gtcggctcca aggacgagcc
     1381 gagggagcgc cggggcacgg cgcacatgtt cgagcacctg atgttcgagg gcaccgcccg
     1441 tgtgcgcccg cagctccacg cccagtcgat cgagtggctc ggcggctacg tcaacgcgtc
     1501 caccgacgag gatgcgacgc actacatcga cacgctgccg cccgagcacc tcgactacgc
     1561 gctccagctc gaggccgacc ggatgcgcgg gctgcggctg accaagccgg cgatcgacgc
     1621 cggccgccag ctgatccagg ccgagctgcg ccagcaggac gcctcgccgt tcgcccaggg
     1681 cctgatccgc tgcctggcgt tcgcctacca gaagcacccc tacgcgtcga tgtcgaccgg
     1741 caacgccaag gagctcgacg cggtgaccgc cgacgagctc aagaagttct acgatgccta
     1801 ttaccagccg aacaacgcgc tgctggtcgt ggtcggcaag gtcacccccg ccgacgtcaa
     1861 ggcgtcggtc gagaagctgt tcggcccgat cgcgcgcggc gccgatccgc cgcgtccggc
     1921 cgacgcgacg cccgagccgg cgcagaccgc caggcgccac caggtcgtcg accccggcgc
     1981 ggtcggcctg accctggtcg gctggcacgt gccgccggcc agggacaagg acatcttccc
     2041 gctgcagctc gcgctcaacg tgctcggcac cggcgatggc tcgcggctca aggcgcggct
     2101 caaggcgccc gatcccaaga ccaagcagcc gctcgcgctc gacgccgcga tggacgcgtt
     2161 catccgcgag cagcccggca tggtgatcgc gctcggcggc tacctcgatc cggccaaggc
     2221 cgacgcggtc gaggccgcgc tggtcgacga ggtcggcaag ctcgcggcga aggggccgac
     2281 ggccgacgag ctgcgcaagg ccaagaacca ggtcgaggcc ggcttcgtgt tctcgctcga
     2341 gaacgcgcag ggcctcggcg aggcgatcgg ccggtcgtgg atcttcaccg gcaaccccgg
     2401 ctcgttcctg tacgaggccg acgagctcga caaggtctcg gcggtcgacg tccagcgcgt
     2461 cgtcaagcaa tacctgtccc ccgaccgcgc caccgtggtg gtcatccctc ccaaggcccg
     2521 atgaacatgc gcacgctcct ggtcaccact gccctcggcg ccgccgcttg caccctcggc
     2581 gcggcctgcg gccccaagcc cgcctcggcg ccgatcccga tgttgcccgg cgacggcgac
     2641 gcccacgtgg tcaagccgcc gccgcccagg ccgccgccgc cccccgatcc gtacgccggg
     2701 cgcagcgacc tgatcgcggt cccctcggtg cgtccgcccg ggccggtcga gctgcccaag
     2761 ctggaggagt ggaagctcgg caacgggctc caggtctacg cggtccggtc gccgcggctg
     2821 ccggtggtcg cgatgcagct cgcggtccgc gccggccgca tgaacgagcc gcgctcccgg
     2881 ctcggggtgt ccgagctcac cgccgacatg ctggtcaagg gcaccaggac ccgcgacgcg
     2941 gcggcgctgg ccaaggcgat cgacttcgtc ggcggcacga tctccgcgga ctcgacgttc
     3001 gaggcgacga tcctgtcgtg cagcgtgctg gcacgcagcg ccggcacctg cttcgacctg
     3061 gtcagcgaca tggtcaccca gccggcgttc cccgacggcg agctgaccgc gctgcgcgac
     3121 tcgctggtcg ccaagatcca gcagcgcacc gaggatccgg cggcgctggc ctcggcgcac
     3181 gcccagaacc tcctgtgggg caacgaccac atccggggct ggatcaacag cgcgcagtcg
     3241 atgggctcga tccggcgcga tgacatcgcc gcctggcacc gcgcgtggtt cgtgccgggc
     3301 aacgcgatgc tggtcgtggt cggcgacatc gatccgaagc ggctgcgcgg cgacctcgag
     3361 cgcacgttcg gcgcgtggcg caaggggccg gtcccgccgg tgccggccta ccccgagcag
     3421 gggctgtcgg gcacccggat ccggctggtc gacaagccgg gcctggccca gacccagatc
     3481 cggatcgcgc agttcgggat caagcacgac gacgcgcggt tcttcgacac gctggtgtgg
     3541 aacttcgcgc tgggcggcgg gcagggctcg cggctggccc gggcggtgcg cggcgacggc
     3601 agcaagggct acgtcgccgg ctcgggcttc gatcgcaaca gcgatcgcgg ctcgttcgtg
     3661 gcgtcgagcg tgacccgcaa tgccgacgcc gtcgcgacga ccaggcagat gatcgccgag
     3721 atcgccaaga tggccaagga cgggccgacc caggacgagg tcggcaccgc gatcgtgagc
     3781 atcaccggcg gctacggcct gcggttccag gccgccgccg acttcggcgg ggcgctggtc
     3841 accgccgagc tccacggctt cggccaggag tacctgacca actacccggt cgcggtcggc
     3901 caggtctcgg tcgccgaggc caagcgcgcc gcggccgaga tcctcgatcc caaggcctac
     3961 gtggtcgtgc tggtcggcga cgccaaggac ctcgagcccg cgctccacaa ggagggctgg
     4021 cgctacgaga agatcgcgta caccgatccg gtctcgccgc cgtacgtcgc gcccgaggcg
     4081 ccggccgacg ccaaggccat cgccgccgcg cgcaagctga tcgacgacgc gctggtcgcc
     4141 aagggcggca aggccaagct cgccgggctc aaggggctca agctggtcgc gaccggcgtg
     4201 agcacgttca gcggccagcc ggtgccggtc gagatcgcgc gcacgctcgc ggtgcccgac
     4261 aagatgcgga tcgacgcgac gctcaagctg ccgcagcccc agggcgacgc ggtggtgtcg
     4321 atcgtcgtcg cgggcaaggc cggctggcag cgcgccccgg acggccgcgg cggctatgcc
     4381 ctggtcgaca tcaccggccc cgcgctggcg accgccgact tcgagcgctg gcgcgagccc
     4441 gagctgatcc tgctcaaggc cgccgagccc gacgccaagc tgacgccgat ggccgacgag
     4501 accgtcgacg gcaagccgtg ttcggtggtc aagctgcgct cgccgctgcc cggcgtcgac
     4561 gtcgagctgt acatcgacaa gaagaccagg atggtcagcc ggatgatcta cagcgacggc
     4621 ggcagcaccg agaccgacga gttctccgac taccgcgacg cctcggggct caaggtcgcg
     4681 tacaggcgcg tcaccagcgg cgccggccgg tcgaccgcgc tggacatcaa gagcgtcgag
     4741 ctcgatccca agatcgagcc gacactgttc gacaagccgg cgccgtccga caagccggcc
     4801 cagtccgaca agccggccca gtccgacaag ccggccaagt gacggccagg gtcggttccg
     4861 tcgccgcgct ggccgcggtg ctcgccgcag ccgtggccgt ggtcgcgcgc gcccagccgg
     4921 cacacctggc ttccggcgag gtcccggccg gcgcggtcga gcccgagccg ccggcgatcg
     4981 agctgctgac cttcggggtc ggcgcgcgga tcttcgagaa gttcgggcac gcggcgatct
     5041 gcctgcgcta tcaccagccc gagcatcccg cgatctgctt caactacggc gtcacgaact
     5101 ttagcgccgg cgcggtgatg gtgtggagct tcctgcgcgg gcagcagaag ttctgggtcg
     5161 agccgacgac gctcgacgcg atgctcgcgt tctaccgggg cgaggatcgc gacatctggc
     5221 gccagacgct gccgatcacc ggcgatgccg cgcgcgcgat cgaggcccgg ctgtggagcg
     5281 acatccgcga ggaccatcgc tactacatgt acgatcactt ctacgacaac tgctcgaccc
     5341 ggctgcgcga catgatcgac caggcgaccg gcggcgcgct gtcggcgagc cgcgccacgc
     5401 gccatccccg gacgttccgc gagctcggcc ggcgcgggct cgccggcatg ccgctgttgc
     5461 tggtgaccag cgatctgatc atcgggcgcg agctcgacca gcacccgacg atgtggcagg
     5521 cgatgttcta ccccgagatc ctgcgcagcg tgggcgcgcg acagctccac gcgccgcccg
     5581 agctgatcta ccaccgccgc ggcccgccgt tcccgatccg gggcaacagc ggccggctgc
     5641 acttcctcgc ggtggcggtc gcgctggcgg tcccgctgtt catcgcccac gcggtgcgcc
     5701 ggcaccaggc cgcggcgctg tgggcggtga gcgtcgcgct cgcgttgctc ggcgtgatcg
     5761 tgtggaccgt ggcgatcgcg tcgtcgatcc cggccgtacg ctacaacgag gcggtgttcg
     5821 tggtcatgcc gctcgacctc gcgctgccgc tgctgtcgcc ggactggcgc cggcgctacg
     5881 cgcggggccg gatcgccgtg ctggcggtgt tcctggcgct cgcgctggtc ggcgtgttcc
     5941 gccagccgct gtggatcgtc gtgctcgcgg tggcgctgcc gatggcgacg gtcgcgttcg
     6001 atctgccgca cggccggcag cgcccggaga ccgcctgatc cacgacgtcg tgatcgtcgg
     6061 ggcggggctg tcggggctgg tgtgcgcgcg gcggctgatc gacgccgggg cgcgggcgat
     6121 cgtcgtcgag gcgcgcccgc gggtcggcgg ccggctgcac ttcctcgcgg tggcggtcgc
     6181 gctggcggtc ccgctgttca tcg
//
LOCUS       DASVOR010001914         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_312582,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001914 DASVOR010000000
VERSION     DASVOR010001914.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_312582"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..182)
                     /locus_tag="VF516_35500"
     CDS             complement(6..182)
                     /locus_tag="VF516_35500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113108.1"
                     /translation="MRDLLIRRVSPALVFTASLGAVGHARHLRGRAGVHVANEALRAR
                     GITVGEVRHACAIR"
     gene            complement(239..586)
                     /locus_tag="VF516_35505"
     CDS             complement(239..586)
                     /locus_tag="VF516_35505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8113109.1"
                     /translation="MFTRSLAAASKSGAPIFAALGDETRLRIVARLSDQGPLSIVRLT
                     EGEAMTRQAITKHLSVLAEAGLVRDVRRGRERLWALEPSPLEVARRYLDLVSERWDTA
                     LARLAKLVESEDG"
     gene            complement(573..1019)
                     /locus_tag="VF516_35510"
     CDS             complement(573..1019)
                     /locus_tag="VF516_35510"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8113110.1"
                     /translation="MTTDRIEKQIVLKASPARVWRALSNAREFGSWFGAEFEDDFAPG
                     KEARGKVRYQGKELTMTFVVERMEPERLLAFRWHPFAIDPTVDYSGEPTTRVELVLTP
                     VPEGTRLTVTESGFDRIPIARRARAFEMNSHGWAQQMENIARHVHA"
     gene            complement(1120..>2238)
                     /locus_tag="VF516_35515"
     CDS             complement(1120..>2238)
                     /locus_tag="VF516_35515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEX8113111.1"
                     /translation="VATGVHQELVRRPAGTIVARSGATTIRNPSVLWFQPRRASETIA
                     VADVPTGTGGSQLETGSEDRRYWGSVYVTLDHGGALTVANAVGEDKLLAGLVPAEMYP
                     DAPAAALEAQAIAARTELLQKIGRRNLTDPFLLCSSQQCQVYAGAGKEDARTTRAIEA
                     TRGIVLLRDGGGLVDVRYSASCGGHGEDNDAIWGGDPDPSLRGRVDRAGGSGGSGAMT
                     RITDGNIGAFLDGERDAWCNQPRLGKGKFRWTETVSAGDLTARIATEHPEVGRVQRLI
                     ARQRGASGRIGVLAIEGDQGRVEIAGDLHIRRLLGGLKSTLFEVHREGDAFVFRGAGF
                     GHGVGMCQMGAIGMASAGRSHAQILAHYFRGTHLHRLY"
BASE COUNT          297 a          910 c          720 g          311 t
ORIGIN      
        1 gctggtcacc ggatggcaca cgcgtggcgt acctcgccga cggtgatccc tcgggcgcgc
       61 agagcttcgt tcgcgacatg gacgccggcg cggccacgca ggtgacgcgc gtgtccgacc
      121 gcgccgagcg aggcggtgaa caccagggca ggggagacgc ggcggatcag gaggtcgcgc
      181 aagcgccccc atcatactcg cgcccgggcc tcgcgccggc cccgcgcccc gggccggctc
      241 agccgtcctc ggactcgacc agcttggcca gccgggccag cgcggtgtcc cacctctccg
      301 acaccagatc caggtatcgc cgtgccacct ccagcggcga cggctccagc gcccacagcc
      361 gctcgcggcc gcgccgcacg tcgcgcacca gccccgcctc ggccagcacc gacaggtgct
      421 tggtgatcgc ctggcgcgtc atcgcctcgc cctcggtcag ccgcacgatc gacagcggcc
      481 cctgatccga cagcctggcg acgatccgga gccgggtctc gtcgccgagc gcggcgaaga
      541 tcggcgcgcc gctcttgctc gccgctgcca ggctacgcgt gaacatggcg cgcgatgttc
      601 tccatctgct gggcccagcc gtgcgagttc atctcgaatg ccctggcgcg gcgcgcgatc
      661 gggatccggt cgaaccccga ctccgtcacg gtcagccgcg tgccctcggg caccggcgtc
      721 agcaccagct cgacccgcgt cgtcggctcg ccggagtagt cgaccgtcgg gtcgatcgcg
      781 aacggatgcc agcgaaacgc cagcagccgc tcgggctcca tccgctcgac cacgaacgtc
      841 atcgtgagct ccttgccctg gtacctcacc ttgccgcgcg cttccttgcc gggggcgaag
      901 tcgtcctcga actccgcccc gaaccagctg ccgaactcgc gcgcgttcga cagcgcccgc
      961 cacacccgcg ccggcgacgc cttgagcacg atctgcttct cgatccgatc ggtcgtcata
     1021 tgcaacctcc tggttgcaga ttaacccggc cacgcagaaa tgcaaccgaa aggttgctga
     1081 tcatcgcagc cgacctctcc gcaccgggcg cgggcggcgt cagtacagcc ggtgcaggtg
     1141 cgtgccgcgg aagtagtgcg cgaggatctg cgcgtgcgac cggccggcgc tcgccatccc
     1201 gatcgccccc atctggcaca tcccgacgcc gtgcccgaac cctgcgccgc ggaacacgaa
     1261 cgcgtcgccc tcgcggtgga cctcgaacag cgtcgacttc agcccgccca gcagccgccg
     1321 gatgtgcagg tcgcccgcga tctcgacccg gccctggtcg ccctcgatcg cgagcacgcc
     1381 gatccgcccc gacgccccgc gctgccgcgc gatcagccgc tgcacccgcc cgacctcggg
     1441 gtgctcggtc gcgatccgcg ccgtcaggtc gcccgccgac accgtctcgg tccaccggaa
     1501 cttgcccttg ccgagccggg gctggttgca ccacgcgtcg cgttcgccgt cgaggaatgc
     1561 gccgatgttg ccgtcggtga tccgcgtcat cgcgccggat ccgccggatc cgccggcgcg
     1621 gtcgacccgg ccgcgcagcg acggatccgg atcgccgccc cagatcgcgt cgttgtcctc
     1681 gccgtgcccg ccgcacgacg cgctgtagcg cacgtcgacc agcccgccgc cgtcgcgcag
     1741 cagcacgatc ccgcgcgtcg cctcgatcgc ccgcgtcgtg cgcgcgtcct ccttgcccgc
     1801 gcccgcgtag acctggcact gctgcgacga gcacaacagg aacgggtcgg tcaggttgcg
     1861 ccggccgatc ttctgcagca gctcggtgcg cgccgcgatc gcctgcgcct ccagcgccgc
     1921 cgccggcgca tcggggtaca tctccgccgg caccagcccg gcgagcagct tgtcctcgcc
     1981 caccgcgttg gccaccgtca gcgccccgcc gtgatccagc gtcacgtaca ccgaccccca
     2041 gtagcggcgg tcctcgctgc cggtctcgag ctgcgagccg cccgtgccgg tcggcacgtc
     2101 ggccacggcg atcgtctcgc tcgcccgccg gggctggaac cacagcaccg acgggttgcg
     2161 gatcgtcgtc gcgccgctgc gcgccacgat cgtcccggcc ggccgccgca ccagctcctg
     2221 gtgcacgccg gtcgccac
//
LOCUS       DASVOR010001915         3072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_370774,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001915 DASVOR010000000
VERSION     DASVOR010001915.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370774"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..700
                     /locus_tag="VF516_35520"
     CDS             <1..700
                     /locus_tag="VF516_35520"
                     /inference="COORDINATES: protein motif:HMM:NF015113.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8113112.1"
                     /translation="MIGAMIMTHSDDDGLVCPPKLAPQQVVIIPVIQKPEGRQQVLDY
                     CAQLKREIEAQSYAGAPVRVHLDARDLQGARKVWEWITKGVPVRLEVGPKDIEKDAVF
                     LGRRDKKPKDKQSMARGEFVAGIAGMLQSIHDALYQRAVAFRVEHTRTIDTKEEFYAW
                     FTPPPTKNPNDPTPIHGGFAMTHFNGDPGLEAQIKDELGVTVRCIPLAPGEPGICPFS
                     GQPSAKRVVWAKAY"
     gene            1130..2560
                     /locus_tag="VF516_35525"
     CDS             1130..2560
                     /locus_tag="VF516_35525"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8113113.1"
                     /translation="MTQAKRDREAPLRISLQNLDEVKLSRTAARCIELRGEGAPRTLR
                     VGVADNWMSLDHATLVREVDRWTVRDYASKNGTKINGQRWRTATLRDGDVVEMGRTFF
                     VFRAEQPTWLDAPQWFDSRELEVLPGLATMVFGLADRFGALARLATTSAPIVIQGPAG
                     CGKELLARAVHMLSGRRGQFVAVNCAGLSEAAAERELFGAVRRAIAGANCEERGLIAA
                     SAGGTLFLDEIADLSPALQSRLLRVLQHRTVRPTGAAAEIRLDLRVVAATRRDLVRLV
                     EHGNFRYDLLDRLGSLFCVPPLCERREDLGLIIEAILERIAGPRARDIELSVGAMRGL
                     MQHAWPGDVRELQAALERAVELAELAPVEIHHLPTEIQRSVPEVLHRAEPSNQLADRS
                     VGTPRMSEDAPEDPITPVNRASDVDPAPSGAGVTSWSRAIAARSFSIRPASSIPEVYA
                     TIACPQGTVPGVARRNSSSASSSDTC"
     gene            2828..>3072
                     /locus_tag="VF516_35530"
     CDS             2828..>3072
                     /locus_tag="VF516_35530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113114.1"
                     /translation="MAGERGQRERAARGLGGLIGRDLVLGVEHHHRGAVGQLLQAAAV
                     GSDRVPQRVLERRVVPGGGAQHHDGQQPALAGGRRARA"
BASE COUNT          469 a         1044 c         1104 g          455 t
ORIGIN      
        1 catgatcggc gcgatgatca tgacccacag cgacgacgac ggcctggtct gcccgcccaa
       61 gctcgcgccg cagcaggtcg tgatcatccc ggtgatccag aagccggagg gccgccagca
      121 ggtgctcgac tactgcgccc agctcaagcg cgagatcgag gcccagagct acgccggcgc
      181 gccggtgcgg gtccacctcg acgcgcgcga tctgcagggc gcgcgcaagg tgtgggagtg
      241 gatcacgaag ggcgtgccgg tccggctcga ggtcggtccc aaggacatcg agaaggacgc
      301 ggtgttcctc ggccgccgcg acaagaagcc caaggacaag cagtcgatgg cgcgcggcga
      361 gttcgtcgcc ggcatcgccg ggatgctgca gagcatccac gacgcgctgt accagcgcgc
      421 ggtcgcgttc cgcgtcgagc acacgcgcac catcgacacc aaggaagagt tctacgcctg
      481 gttcacgccg ccgccgacca agaaccccaa cgatccgacg ccgatccacg gcggcttcgc
      541 gatgacccac ttcaacggcg atcccgggct cgaggcccag atcaaggacg agctcggcgt
      601 caccgtgcgc tgcatcccgc tcgctcccgg cgagcccggt atctgtccgt tcagcggtca
      661 gccgagcgcc aagcgcgtgg tgtgggcaaa ggcgtactga cacccttcac ggacagtcac
      721 caaccgagct gactccggag ttgcgctgga tcgcgaagcc cagctccgtc gcacctgtcg
      781 cgccgccgtc tgtcggggcg cgtcatggca gagatcggaa catgggctgt cgatcgtgcg
      841 atgtatggac atggccgacg gccgtgaggc aggaccgcac gcggtgattc acgcctcacg
      901 gctgccgcac tgcagcggcg gtcgtccaga agttcgtcag actggaacaa ttcatatctg
      961 atggcgttac aacgtaatgg gggcaaccct gggggttggt ccggcgtccg ttgagtgagt
     1021 aatttttcgg ggggtgggtg tgatggactt tggcgttgat acgactctgt ccttggtgac
     1081 cggtggaagc tcgtcccgga ggttcggtgt cgcttctcac ctgtttctcg tgacccaggc
     1141 gaagcgcgac cgcgaggccc cgctgcgcat ctcgctgcag aacctcgacg aggtgaagct
     1201 gtcacgcacc gccgcgcgct gcatcgagct ccgcggtgag ggtgcacctc gcacgcttcg
     1261 ggtcggtgtc gccgacaact ggatgtcact cgatcatgca accctggtcc gggaagtcga
     1321 tcgctggacg gtccgcgact acgcctcgaa gaacgggacg aagatcaacg ggcagcgatg
     1381 gcgaacggcg acgctgcgcg acggcgacgt ggtggagatg ggacgcacgt tcttcgtgtt
     1441 tcgcgccgag cagccgacgt ggctggacgc gccgcagtgg ttcgacagcc gcgagctgga
     1501 ggttctcccc ggactggcca ccatggtgtt cgggctggcg gatcgcttcg gcgcgctggc
     1561 tcgcctcgcc accacgagcg cgccgatcgt gatccagggg ccggccggat gcggcaagga
     1621 gctgctcgcg cgcgccgtgc acatgctgtc cggtcgccgg ggccagttcg tcgcggtgaa
     1681 ctgcgccgga ctgtccgagg ccgccgccga gcgcgagctg ttcggggccg tgcgccgggc
     1741 gatcgctggc gcaaactgcg aggagcgcgg gctgatcgca gcatcggccg gggggacgct
     1801 gttcctcgac gagatcgccg acctgtcgcc ggccctgcag agccggctgc tgcgcgtgct
     1861 gcagcaccgg acggtgcggc cgaccggcgc cgccgccgag atccggctcg atctgcgggt
     1921 cgtcgctgcg acccgccggg atctcgtccg gctggtcgag cacggcaatt tccgttacga
     1981 cctgctggat cggctcggca gcctgttctg cgtacccccg ctgtgcgaac gccgcgagga
     2041 cctcgggctg atcatcgagg cgatcctcga acgcatcgca gggccgcgcg cccgggacat
     2101 cgagctgtcc gtcggggcga tgcgagggct gatgcagcat gcctggccgg gcgacgtgcg
     2161 cgagctgcag gccgccctcg agcgagcggt cgagctcgcc gagctcgctc cggtcgagat
     2221 ccatcatcta cccacggaga tccagcggtc tgtcccggag gtcctgcacc gggccgagcc
     2281 ctcgaaccag ctcgccgacc gctctgtcgg cacgccgcgg atgtcggagg atgcaccgga
     2341 ggacccgatc actccggtga acagagccag cgacgtcgat ccggcgccgt cgggagccgg
     2401 ggtgacgtcg tggagccgcg cgatcgctgc caggagcttc tcgatccggc cggcgagcag
     2461 catccccgag gtgtacgcga ccatcgcctg cccccagggg acggtgccgg gcgtcgccag
     2521 gcgcaacagc tcctcggcct cgtccagcga tacctgctga gcgacgtccg ctgcctcgca
     2581 gtggaaaccg agcagggcga tccggagctc gggcggcggt gcgcaggcca ggccgagact
     2641 ggcgcggctc gatcacgtcg aaccttccgt cgagcaggcc ggcgctgacc tcgtcgagcg
     2701 cgatcggcgg caggtcggct ggctgccggg ccatgtctgc gagcagccgc gaggcccgct
     2761 cgatcacgtc gccttcgggc agccggctgc ccgactcggc gcggcccatc gcgatcgcca
     2821 gggggcgatg gccggtgagc gcggacagcg cgagcgcgca gcgcgcggcc tgggcggctt
     2881 gatcggtcgc gacctggtgc tcggcgtcga gcatcaccac cgcggagccg tcggccagct
     2941 gctccaggcg gccgccgtag ggagcgaccg cgtgccgcaa cgggtcctcg agcggcgggt
     3001 cgtcccgggc ggcggggccc agcatcacga cggccagcag ccggcgctcg ccggtggtcg
     3061 ccgagcgaga gc
//
LOCUS       DASVOR010001916         4260 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_487767,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001916 DASVOR010000000
VERSION     DASVOR010001916.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487767"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VF516_35535"
     CDS             complement(<1..1079)
                     /locus_tag="VF516_35535"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113115.1"
                     /translation="MSRAAAEVGSTENSYQVLAKLASGGMAEIFLARGEGVAGVERYC
                     VLKRILRDRASDVQFVRMFLDEARLAAQLQHPNIAQVYDIGKLGDSYFFTMEYVHGET
                     VRALLHRAVELRRQLPIGCVLTIVAGAAAGLHHAHVRVGLDGRALGIVHRDVSPSNLM
                     VSYEGSVKLVDFGVAKASDRAYETKSGTVKGKISYLSPEQCRGKRVDRRSDLFSLGIC
                     FWEMLTAERMYRRASDFENMNAIVSEPPPPPSSRRADIPPELDELVLRLLAKDPGERC
                     QTADEVVEAVEAFAAGTGSVLSASALGRLVREMFGPRREPWLELKRLHRQGEGVTVTG
                     EPIPYELAIPPADRLDHQLASVIDL"
     gene            1379..1516
                     /locus_tag="VF516_35540"
     CDS             1379..1516
                     /locus_tag="VF516_35540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113116.1"
                     /translation="MKVTRLSIIAALLAATVTGCIVHTAPCYHDCWWSHGHRVCARRC
                     Y"
     gene            complement(1640..2755)
                     /locus_tag="VF516_35545"
     CDS             complement(1640..2755)
                     /locus_tag="VF516_35545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113117.1"
                     /translation="MTHQPVAWKMVTAPDLKSVDLMIKGAYSVAVVCMINPTTVLVWQ
                     AFHTVDDDTSSTVKEPTLQTPCNAAPTQRTVHGTTVQDASVHLGNADASATGGTPFTV
                     QVADGTYDLVATTKETDPATHKTLIQRNVMVSGDTTLPGTIDVSTGVAQVALGVVLSS
                     PPNPAKSTETVAATVDVTTKNNKGSATVSSADYNLTNKTIKIFALPNSALMSGDTQTA
                     TFTGTNNIKDTSITTSRSITRPFSIGDAVATGMTSFALPAAIANPDWGMDKSRLSVAL
                     PTLPALDDLTVKTSGKTADGQTTVQYEIDITASYFQRTALARPVFDTDIPGFQPSWKI
                     DFVHQAYSRQITSERDVFSKGAFVDHEESSFFEDVTP"
     gene            complement(3348..>4260)
                     /locus_tag="VF516_35550"
     CDS             complement(3348..>4260)
                     /locus_tag="VF516_35550"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113118.1"
                     /translation="PALRGPLAEIEHALAAGRVDRALAAARAWHDREPGDVLALIGLG
                     DALEARHDLETAARVYGSIIDLYPARADFRRFAGERLERVGKVQRALIIDTYRRAVEQ
                     RPDHLTGHRLLAYALVSDGQYAAAFAAILDGIDRRYPDNRFAGAERVLAEDVGMIGAA
                     YLAHVPGKRDAVMAELDRRGIALPTRPSTRFILYWETDGNDVDFHIRDARGGHAWYGH
                     RQLRSGGELYADITTGYGPECFAIPGTPAAGPYRLSINYFSQGPMGYGMGLLQVQTFD
                     GRGNLRLEDRPYVIMTDQAFVDLGTYR"
BASE COUNT          660 a         1438 c         1462 g          700 t
ORIGIN      
        1 ctgaggtcga tcacgctcgc cagctggtgg tcgagccggt cggccggcgg gatcgcgagc
       61 tcgtacggga tcggctcgcc ggtcaccgtc accccctcgc cctggcggtg cagccgcttg
      121 agctccagcc agggctcgcg gcgcggaccg aacatctcgc gcacgagccg gccgagcgcc
      181 gacgccgaca gcaccgagcc ggtccccgcc gcgaacgcct cgaccgcctc gaccacctcg
      241 tccgcggtct ggcaccgttc gccggggtcc ttggcgagca gccgcagcac cagctcgtcg
      301 agctccggcg ggatgtcggc gcggcgcgac gacggcggcg gcggcggctc gctgacgatc
      361 gcgttcatgt tctcgaagtc actcgcccgc cggtacatcc gctcggcggt cagcatctcc
      421 cagaagcaga tgccgagcga gaacagatcg ctgcggcggt cgacccgctt gccccggcac
      481 tgctccggcg acaggtagct gatcttgccc ttcaccgtgc ccgacttggt ctcgtacgcg
      541 cggtccgagg ccttggcgac cccgaaatcg accagcttga cgctgccctc gtagctcacc
      601 atcaggttcg acggcgacac gtcgcggtgc acgatgccga gcgcgcggcc gtcgaggccg
      661 acccggacgt gcgcgtggtg caggcccgcc gccgcgcccg cgacgatggt cagcacgcag
      721 ccgatcggca gctggcgccg gagctccacc gcgcggtgga gcagcgcgcg cacggtctcg
      781 ccgtgcacgt actccatggt aaagaagtac gagtcgccga gctttccgat atcgtagacc
      841 tgcgcgatgt tcggatgctg gagctgcgcg gcgagccggg cctcgtcgag gaacatccga
      901 acgaactgca catcgctcgc ccgatcgcgc aggatccgct tgagcacgca gtagcgctcg
      961 acgccggcga ccccctcgcc gcgggccagg aagatctctg ccattccgcc gctcgcgagc
     1021 ttggcgagga cctggtagct gttctctgtc gatccgacct ctgcggcagc cctcgacacg
     1081 tctcattatt gttcgaactt gtgccgcgcg ggttgccaat ccatggtcaa acgaccccaa
     1141 cggaacggct ccgccgcggt gagactgcct gcaagccctc gcagttccgg gcgaatcggc
     1201 ggcttgtcag gagaaactcg cggtctcgtt tttacgatgt cgtccgcgac cgagcgtcac
     1261 cgcgcgtcac cgcgcaggga ggtgggcaat ccgtacgatg cttcgagtcg ggaccagtaa
     1321 accagtcgta cccgctgtgg gcccctcgga cagggaggcc gtccgccccg gagttcccat
     1381 gaaggtcacc cgactctcca tcatcgccgc cttgctcgcg gcgacggtca ccggctgcat
     1441 cgtccacacc gccccctgct atcacgactg ctggtggagc cacggccacc gggtctgcgc
     1501 gcgccgctgc tactgatccc gaccgcggtc cacctcgccg cccgaccgag tgccggccca
     1561 gccgagacga ggtcgagagc agaatcgaca gggcgtgcga cggcgcgcgc caccccgcgc
     1621 gccggcgcac gaatgcggat tacggcgtga cgtcctcgaa gaacgagctc tcctcgtggt
     1681 cgacgaacgc gcccttgctg aacacgtcgc gctccgacgt gatctggcgc gagtacgcct
     1741 ggtggacgaa gtcgatcttc cagctgggct ggaagccggg gatgtcggtg tcgaacaccg
     1801 gacgcgccag cgcggtccgc tggaagtagc tcgccgtgat gtcgatctcg tactgcacgg
     1861 tggtctgccc gtcggccgtc ttgccggacg tcttgacggt gaggtcgtcg agcgcgggca
     1921 gcgtgggcag cgccacgctc agccggctct tgtccatgcc ccagtccggg ttcgcgatcg
     1981 cggcgggcag cgcgaagctg gtcatcccgg tggcgaccgc atcaccgatg ctgaacggcc
     2041 tggtgatcga gcgcgaggtg gtgatgctgg tgtccttgat gttgttggtc ccggtgaacg
     2101 tggcggtctg cgtgtcgccc gacatgagcg ccgagttggg caacgcgaag atcttgatcg
     2161 tcttgttcgt caggttgtag tccgcagagg agaccgtcgc cgagcccttg ttgttcttgg
     2221 tcgtgacgtc gaccgtggcc gccaccgtct cggtgctctt ggccgggttc ggcgggctgg
     2281 agagcacgac cccgagcgcg acctgcgcca cgccggtcga gacgtcgatc gttccgggca
     2341 gcgtggtgtc gccactgacc atgacattgc gctggatcag ggtcttgtgc gtcgccgggt
     2401 cggtctcctt cgtcgtcgcg acgaggtcgt aggtcccgtc ggcaacctgc acggtgaacg
     2461 gggtgccccc ggtcgccgag gcgtcggcat tgccgaggtg gacggaggcg tcctggaccg
     2521 tcgtgccgtg gaccgttcgc tgggtcggcg ccgcgttgca cggcgtctgc agcgtgggct
     2581 ccttcaccgt gctcgacgtg tcgtcgtcga cggtgtggaa cgcctgccag accagcacgg
     2641 tcgtggggtt gatcatgcac acgaccgcga ccgagtacgc tcccttgatc atgaggtcga
     2701 ccgacttgag gtcgggcgcc gtcaccatct tccacgcgac cggctggtgc gtcatggggt
     2761 ccgtgaagcc atcgcggtac gcgaccagta acggggtggt gccagatttg atgtgaacag
     2821 gcgtcggcgt cgggtccggt acgaccggag cctcaggcag gctgtcactg cagccaatta
     2881 cggcaggcgc cagcaaggtg caaagcaaga aataagaggg cctcatgggg ggcccatcac
     2941 agcaaaacga gcgccaactt acaagatctg ccccatgcca aattcaaaga tgtcacgtgg
     3001 caaagcagct cacgttgccg ttcagcgccg caagctacgt tacctcgatt tcaggcatag
     3061 aaacatacct gcacaaacca ccctgtcgtg ttcagcgtga cattgaggat acggtatgac
     3121 atccacgtct cggttggcgt ggtactgcgg atacagtggg acgcccccga agcggagcca
     3181 ggccggtgtg cccgagatcg gcgaatgttg gctaggtcgt ggtttcggtg gtcagatctc
     3241 cgaggtaggg cagcgcggca acgcacgtat ccagggctcg ctgcgagaat cctgcaagca
     3301 acatgcccgt ggcaatacgc cccggctccc tgcggggcaa ggggcgtcta ccgataggtg
     3361 ccgaggtcca cgaacgcctg gtccgtcatg atgacgtacg gccgatcttc caggcgaagg
     3421 ttgcctcggc catcgaacgt ctggacctgc agcagcccca tcccgtaccc catcgggccc
     3481 tgtgagaagt agttgatcga gagccggtag gggccggccg ccggcgtgcc ggggatcgcg
     3541 aagcactcgg ggccgtagcc ggtcgtgatg tcggcgtaga gctcaccgcc cgaccgcagc
     3601 tgccggtggc cgtaccaggc atggccgccc cgtgcgtccc ggatatggaa gtcgacgtcg
     3661 ttgccgtcgg tctcccagta caggatgaag cgggtcgacg gccgggtcgg cagcgcgatg
     3721 ccgcgccggt cgagctccgc catcaccgcg tcgcgcttgc ccgggacgtg cgcgaggtag
     3781 gctgcgccga tcatcccgac gtcctcggcg agcacgcgct cggcgccggc gaaccggttg
     3841 tccgggtagc gccgatcgat cccgtcgagg atcgccgcga acgccgcggc gtactgaccg
     3901 tccgagacca gcgcgtacgc cagcaagcgg tggccggtca ggtgatcggg gcgctgctcg
     3961 acggcgcgcc ggtaggtgtc gatgatcagc gcgcgctgca ccttgccgac ccgctcgagc
     4021 cgctcgccgg cgaaccggcg gaagtcggcg cgcgccgggt acaggtcgat gatcgagccg
     4081 tagacccggg ctgcggtttc gagatcgtgc cgggcctcca gcgcgtcgcc gagcccgatc
     4141 agcgcgagca cgtcgccggg ctcccggtcg tgccaggcgc gagcggcggc gagcgcgcga
     4201 tccacgcggc cggcggcgag cgcgtgctcg atctccgcca gcggaccgcg gagcgccggc
//
LOCUS       DASVOR010001917         3278 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341029,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001917 DASVOR010000000
VERSION     DASVOR010001917.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341029"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1754
                     /locus_tag="VF516_35555"
     CDS             <1..1754
                     /locus_tag="VF516_35555"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8113119.1"
                     /translation="TAARVDLDVVGLLRAAEALAREVELPRLLEQLMRICLETAGADH
                     GLLILDEEGVPFVRSAATATAVSLERTPLEAVPDIARAVVDHVRRTGDLVVLDDARRG
                     AFAGHAHASPSPVKSVLAVPIQRPNRAVGVLYLENTLVVEAFSPERVRSLDLLSGHIS
                     VALENSLLFEERRREELATQLLADASSALVESLDYAATLARVAQLAVPALADMCAVDV
                     VEDGQLRRVAVAHIDPAQEPLIFEAGASDLDPGSPHPVAQVLRTGRPCLVSEIEDALL
                     NAQMREPREAAISAVLGARSGIAVPLFARGRILGAMSFMSRQPGRYTARDLPLAQELG
                     RRAGMAIDNARLYAESQAAVQVRDQFLSVASHELRTPLTSLNLTVQALLDHTLPATPD
                     NLRRTFSIAHRQIARLSHLVDELLSVSRITAGCLALELDDVDLAAVVRDVVKRFEVEA
                     SRTGTRVAIHATPVIGRWDRTALDQVVTNIVSNALKFGAGKPIDLTVEAANGTARLVV
                     TDRGIGIPPDRLPHIFERFERAVSARQYGGLGLGLYIARAIIEAFGGSIEARSPGPHA
                     GATFTVKLPRRRLEGLT"
     gene            1751..2146
                     /locus_tag="VF516_35560"
     CDS             1751..2146
                     /locus_tag="VF516_35560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113120.1"
                     /translation="MNHDGTVLVVEDDLDIREVMRMVLEASGYQVLEAGDGAEALEVA
                     RAHRPRIILLDLMMPGMDGFQFRESQLQDPAIASIPVVIVSGGGAVPQKAAELGAAGY
                     LVKPTDVQRLLAVMGDLCRAGGEAQPRVS"
     gene            complement(2139..2600)
                     /locus_tag="VF516_35565"
     CDS             complement(2139..2600)
                     /locus_tag="VF516_35565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113121.1"
                     /translation="MKSANRGRASMLLSKAIAEAAVAVATQLVGQRLYAAYDAMRRYE
                     AMRRRPSAGPGEDEERLPPTPEQVVERLVEEVIERSKQAFERHRPVLARARARTRVGL
                     QQPAIAAVVVGGSVVGAVSALGVVPFALGAGAAYLVYRRLQPEDGAARPAS"
     gene            complement(2672..>3278)
                     /locus_tag="VF516_35570"
     CDS             complement(2672..>3278)
                     /locus_tag="VF516_35570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113122.1"
                     /translation="HAGRRHDVRTALAGGHAYVSEKTGSLEAFALRADGNLDGAGVPV
                     AGIPAGTIVGITGAEDLIVSPVAHLASNANQATVPVSSGATKVQQVETKEVAACWAAS
                     DDGEVCITNPGSMTVSCGHIGAGGFTSYTGAAASPIGESVLDLDVRHGTVGILGTHNG
                     ARVMMVYARAEDDSDFLTAITELPVGTAVATGALVLPPLSR"
BASE COUNT          428 a         1200 c         1176 g          474 t
ORIGIN      
        1 cgaccgccgc gcgcgtcgat ctcgacgtcg tcggcctgct ccgggccgcc gaggcgctgg
       61 cgcgcgaggt cgagctaccg cggctgctcg agcagctgat gcggatctgc ctcgagaccg
      121 caggcgccga tcatggcctg ctgatcctcg acgaggaagg agtcccgttc gtgcggtccg
      181 ccgcgaccgc gaccgcggtg tcgctcgagc gcaccccgct cgaggccgtg cccgacatcg
      241 cgcgtgcggt cgtggaccac gtgcgccgga ccggcgacct ggtcgtgctc gacgatgcgc
      301 ggcgcggcgc gttcgcgggc catgcgcatg ccagcccgag cccggtgaag tccgtgctgg
      361 ccgtgccgat ccagcgcccg aaccgcgcgg tcggcgtgct ctacctcgag aacacgctgg
      421 tcgtcgaggc gttctcgccg gagcgcgtgc gctcgctcga tctgctgtcg ggtcacatct
      481 cggtcgcgct cgagaacagc ctgctgttcg aggagcggag gcgcgaagag ctcgccacgc
      541 agctgctggc cgacgcgagc tccgccctgg tcgaatcgct ggactacgcc gcgacgctcg
      601 cgcgggtcgc gcagctcgcg gtcccggcgc tcgccgacat gtgcgcggtc gatgtcgtcg
      661 aggacggtca gctccgccgg gtcgccgtgg cgcatatcga tccggcgcag gagccgctga
      721 tcttcgaggc cggggcgagc gatctcgatc ccggatcgcc gcatccggtc gcccaggtgc
      781 tgcgcacggg gcggccctgc ctcgtcagcg agatcgaaga cgcgctgctg aacgcgcaga
      841 tgcgcgagcc gcgcgaggcg gcgatctccg ccgtcctcgg cgcccgcagc ggcattgcgg
      901 tgccgctgtt cgcgcgcggt cgcatcttgg gcgcgatgtc cttcatgtcc cggcagcccg
      961 ggcgctacac cgcgcgcgat ctcccgctcg cccaggagct cggacggcgc gccgggatgg
     1021 cgatcgacaa cgcccggctg tacgcggagt cccaggccgc ggtccaggtt cgcgatcagt
     1081 tcctgtcggt cgcgtcgcac gagctacgca ctccgctgac ctcgctgaac ctgaccgtgc
     1141 aggcgctgct cgaccacacc ctcccggcga cgcccgacaa cctgcgcagg acgttctcga
     1201 tcgcgcaccg ccagatcgcg cgactgagcc acctcgtcga cgagctgctc agcgtgtcgc
     1261 gcatcaccgc cgggtgtctc gcgctcgagc tcgacgacgt cgatctggcg gccgtggtcc
     1321 gcgacgtggt gaagcgcttc gaggtcgagg cgtcgcggac cgggacccgc gtggcgatcc
     1381 acgccacccc ggtgatcggc cgctgggaca ggaccgcgct cgaccaggtg gtcacgaaca
     1441 tcgtgtccaa tgcgctcaag ttcggcgccg gcaagccgat cgacctgacg gtcgaggcgg
     1501 cgaacggcac cgcccgcctc gtggtcaccg accgcggtat cgggatcccg cccgatcggc
     1561 tgccccacat cttcgagcgc ttcgagcgcg cggtgtccgc ccgccagtac ggcggcctcg
     1621 ggctcgggct gtacatcgcc cgcgcgatca tcgaggcgtt cggcggatcg atcgaggcgc
     1681 gcagccccgg accccacgca ggggcgacgt tcaccgtcaa gctgccccgc agacggctcg
     1741 agggattgac atgaaccacg acggaaccgt cctggtcgtc gaggatgatc tcgacattcg
     1801 cgaggtcatg cggatggtgc tcgaggccag cggataccag gtgctcgagg cgggcgacgg
     1861 cgccgaggcc ctcgaggtcg cgcgcgcaca ccggccccgc atcatcctgc tcgatctcat
     1921 gatgccgggc atggatggct tccagttccg cgagtcgcag ctgcaggatc cggcgatcgc
     1981 gtcgatcccg gtggtcatcg tctccggggg cggcgccgtg ccgcagaagg cggccgagct
     2041 cggcgcggcc gggtacctgg tcaagccgac ggatgtgcag cgcctgctcg cggtgatggg
     2101 cgacctgtgt cgagcgggcg gcgaggcgca gccgcgggtc agctagccgg tcgcgccgcg
     2161 ccgtcctcgg gctggagcct gcggtacacc aggtaggcgg ccccggcgcc gagggcgaac
     2221 gggaccacgc cgagcgccga gaccgcaccg acgacggagc cgccgacgac cacggccgcg
     2281 atggcgggct gttgcaggcc gacgcgcgtc cgggcacgcg cgcgcgcgag caccggacgg
     2341 tgccgctcga acgcttgctt gctgcgctcg atgacctcct ccaccaggcg ttccaccacc
     2401 tgctcggggg tcggaggcag tcgctcctcg tcctcgcccg gaccggcgct cggccgtcgt
     2461 cgcatcgcct cgtaccggcg catggcgtcg tacgcggcgt acaggcgctg gccgaccagc
     2521 tgcgtcgcga ccgcgacggc cgcctcggcg atcgccttgc tcagcagcat gctcgcacgt
     2581 ccacggttcg ccgacttcat gggctgctcc caatccagtc gaagtgcaga ccgcatgcca
     2641 ccggcggtgc gcacggccgc gggtcgcgcc cctaccgcga cagcggcggc aggaccagcg
     2701 ccccggtggc gaccgcggtg ccgaccggca gctcggtgat cgccgtcaag aagtcgctgt
     2761 cgtcctcggc gcgcgcatag accatcatca cgcgcgcgcc gttgtgcgtg cccaggatgc
     2821 cgaccgttcc atggcggacg tcgaggtcga gcaccgactc gccgatcggg ctggccgcgg
     2881 cgccggtgta gctggtgaag ccgccggcgc cgatgtggcc gcacgacacc gtcatgctgc
     2941 cggggttggt gatgcagacc tcgccgtcgt cgctcgcggc ccagcacgcc gcgacctcct
     3001 tggtctcgac ctgctgcacc ttggtggcgc cgctcgacac cggcacggtc gcctggttcg
     3061 cgttgctcgc caggtgcgcg accggcgaga cgatcaggtc ctcggcgccg gtgatcccga
     3121 cgatcgtgcc ggccgggatg ccggcgaccg gcacgccggc gccgtcgagg ttgccgtcgg
     3181 cgcggagcgc gaacgcctcg agcgagccgg tcttctcgga gacgtaggcg tggccgccgg
     3241 cgagcgcggt gcgtacgtcg tgacgccgac ccgcgtgc
//
LOCUS       DASVOR010001918         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_49677,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001918 DASVOR010000000
VERSION     DASVOR010001918.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49677"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /gene="clpX"
                     /locus_tag="VF516_35575"
     CDS             <1..750
                     /gene="clpX"
                     /locus_tag="VF516_35575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX8113123.1"
                     /translation="HDIERAQRGIVYIDEIDKIARKSDNPSITRDVSGEGVQQALLKI
                     IEGTTASVPPKGGRKHPQQEFLQVDTTNILFICGGAFTGLEDIIENRIGQKLIGFGAG
                     MNKKVERTQWELIQEVEPEDLLKYGMIPEFIGRLPVIAPLHELSEEALVQILTQPKNA
                     LVKQYQKLFEMDGVKLKFGKGALTKVANMAALQKSGARGLRAILESALLDIMYDTPGQ
                     SVSEVIINEDVIEKHGSPLITYSKDSKVAEG"
     gene            complement(761..1879)
                     /locus_tag="VF516_35580"
     CDS             complement(761..1879)
                     /locus_tag="VF516_35580"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8113124.1"
                     /translation="MTPPASLGRGLGRSFALATVIGMVVFAVVQALVIYITEIGEECA
                     PGVPEDPPVEIVEQCAVALAIAAPFGVVLSLKLGRRLTNPTTARLDEVIASAKRMTGE
                     RLDERLPISPNGDALDRLSVALNDVLARVQRGVAAQQQFAADASHELRTPLAVISANL
                     EVARRKPRDAPHWEHVADGTLAEVHRMTTLVDKLLQLSRAGAAGLHQERTDLRQLAGT
                     AAERATQQGQERGVKVELAVGRPITAEVDPEAIAIVIDNLIRNAIDHSAADQTVTVSV
                     APGPTIAVEDRGPGVVPELRTRIFEPFARGRATDRASGTGLGLGLAICKRIVDGHGGT
                     IGVEDRSGGGARFIVRLPASRNDDRLTPIHGAAAVQPA"
     gene            complement(1876..>2202)
                     /locus_tag="VF516_35585"
     CDS             complement(1876..>2202)
                     /locus_tag="VF516_35585"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEX8113125.1"
                     /translation="ALARRAAGPRWAPATDMPLTMRDDLVIESGDRTVALSPREYALF
                     GCLLRRRGEVVARAEILRDAFGYEFDPGTNVIDVHLNHLRKKLQGFPVAIETVRGAGI
                     RLVVSE"
BASE COUNT          343 a          788 c          781 g          290 t
ORIGIN      
        1 cacgacatcg agcgcgccca gcgcggcatc gtctacatcg acgagatcga caagatcgcc
       61 cgcaagagcg acaacccgtc gatcacccgc gacgtgtccg gcgagggcgt gcagcaggcg
      121 ctgctcaaga tcatcgaggg caccaccgcc tcggtgccgc ccaagggcgg ccgcaagcac
      181 ccgcagcagg agttcctcca ggtcgacacg accaacatcc tgttcatctg cggcggcgcg
      241 ttcaccggcc tcgaggacat catcgagaac cggatcggcc agaagctgat cgggttcggc
      301 gccggcatga acaagaaggt cgagcgcacc cagtgggagc tgatccagga ggtcgaaccc
      361 gaggatctgc tcaagtacgg gatgatcccc gagttcatcg gccgcctgcc ggtgatcgcg
      421 ccgctccacg agctgtcgga ggaggcgctg gtccagatcc tgacccagcc caagaacgcg
      481 ctggtcaagc agtaccagaa gctgttcgag atggacggcg tcaagctcaa gttcggcaag
      541 ggcgcgctga ccaaggtcgc caacatggcc gcgctgcaga agagcggcgc gcgcggcctg
      601 cgcgccatcc tcgagagcgc gctgctcgac atcatgtacg acacccccgg ccagtcggtg
      661 agcgaggtga tcatcaacga ggacgtgatc gagaagcacg gctcgccgct gatcacgtac
      721 agcaaggatt cgaaggtcgc cgagggttga ccgcccccga tcaggcgggc tgcacggccg
      781 ccgcgccgtg gatcggcgtc aggcggtcgt cgttgcgcga cgccggcagc cgcacgatga
      841 accgcgcgcc gccgccggag cggtcctcga cgccgatcgt gccgccgtgg ccgtcgacga
      901 tccgcttgca gatcgccaga cccaggccga gcccggtgcc ggaggcgcga tcggtggcgc
      961 ggccgcgcgc gaacggctcg aagatccggg tccgcagctc gggcaccacg ccggggccgc
     1021 gatcctcgac cgcgatcgtc ggcccgggcg cgacgctgac cgtgaccgtc tggtcggccg
     1081 ccgagtgatc gatcgcgttg cggatcaggt tgtcgatcac gatcgcgatc gcctcggggt
     1141 cgacctcggc ggtgatcggg cggccgaccg cgagctcgac cttgacgccg cgctcctggc
     1201 cctgctgcgt ggcgcgctcg gccgcggtgc cggcgagctg gcgcaggtcg gtgcgctcct
     1261 ggtgcaggcc cgccgcgccg gcgcgcgaca gctggagcag cttgtcgacc agcgtggtca
     1321 tccggtgaac ctcggcgagc gtcccgtcgg cgacgtgctc ccagtgcggt gcgtcgcgcg
     1381 gctttctgcg cgcgacctcg aggttggccg agatcaccgc gagcggcgtg cgcagctcgt
     1441 gcgacgcgtc ggccgcgaac tgctgctgcg ccgcgacccc gcgctggacc cgcgcgagca
     1501 cgtcgttgag cgccaccgac agccgatcga gcgcgtcgcc gttggggctg atcggcaggc
     1561 gctcgtcgag ccgctcgccg gtcatccgct tggcgctcgc gatcacctcg tcgagccgcg
     1621 ccgtggtcgg gttggtcagc cggcggccga gcttgagcga cagcacgacg ccgaacggcg
     1681 ccgcgatcgc gagcgcgacc gcgcactgct cgacgatctc gaccggcggg tcctccggca
     1741 cccccggcgc gcactcctcg ccgatctcgg tgatgtagat gaccagcgcc tggacgacgg
     1801 cgaacaccac catgccgatc acggtggcga gcgcgaacga ccgcccgagg ccgcggccga
     1861 gcgaggccgg cggcgtcatt cggacaccac gagccggatc ccggcgccgc gcacggtctc
     1921 gatcgcgacc gggaagccct ggagcttctt gcgcaggtgg ttgaggtgga cgtcgatcac
     1981 gttggtcccc ggatcgaact cgtagccgaa cgcgtcgcgc aggatctcgg cgcgcgcgac
     2041 gacctcgccg cgccggcgca gcaggcagcc gaacagcgcg tactcgcgcg gcgacagcgc
     2101 caccgtccgg tcaccggact cgatcaccag gtcgtcgcgc atcgtcagcg gcatgtcggt
     2161 cgccggggcc cagcgcggcc cggcggcgcg ccgcgcgagc gc
//
LOCUS       DASVOR010001919         1987 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_370831,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001919 DASVOR010000000
VERSION     DASVOR010001919.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1987
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370831"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VF516_35590"
     CDS             complement(<1..823)
                     /locus_tag="VF516_35590"
                     /inference="COORDINATES: protein motif:HMM:NF028237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HEX8113126.1"
                     /translation="MFDLRWSWLCVPYLACAAALCAVGVVAGLVRGDRVMRLGMIGAA
                     TAGLPWTIGSALASCTDDPEVAVRLLRLGAGPVSLIGPNLLLVLLGISGQLERHRWVV
                     AASGAVGTALLGLCWGTSWTVPGVHHLTSGVFYPTGGPLTDIHIGQLAMWIAIGLIIA
                     RRSMMRGERRRMLRVLVAALVLAAIGATDVLLVHGVVGAYPIAWLPGTIACGIVLYLE
                     LRTDLLRPTGVDRGALLELAGLAATAALVALIAYVLHGAAAVAVAAFASAVWLAVT"
     gene            908..1582
                     /locus_tag="VF516_35595"
     CDS             908..1582
                     /locus_tag="VF516_35595"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8113127.1"
                     /translation="MSRRSPRYRIDTAATAVPLLGGDIAACRTHDVSESGLSLDTAAW
                     FPLGTRLAVALVDPTTGSAIEVIGDVVREAAPPSWTLGILLIDPPLEWQGVVATAARR
                     SGNAPVKPTKRMRVLVFGDDHRQRGAMALYVTSGWDVLFATDEDSIAEALAHIELDAV
                     VAELDVTDPRLPPIMHSVRRSQPSARRIARGTGRAETELVHRFVDRDAGLDALLDAVT
                     ADIPVG"
     gene            complement(1601..>1987)
                     /locus_tag="VF516_35600"
     CDS             complement(1601..>1987)
                     /locus_tag="VF516_35600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113128.1"
                     /translation="AAWMTRTFEEFWADHGAPTVTLNNTFLEPMTPAGRLLMISQCGS
                     DGVTDSPRQRIADAIFENLVDPRRITDAFVDPRAARKLIAELTGHSWRRAFLTGVLRV
                     GSNQLRRVFGRAPASSGTPAQPATPR"
BASE COUNT          292 a          752 c          695 g          248 t
ORIGIN      
        1 cggtcaccgc cagccacacc gcgctcgcga acgcggcgac cgcgaccgcc gcggcgccgt
       61 gcaggacata cgcgatcagg gcgaccagcg ccgcggtcgc ggccagcccc gccagctcga
      121 gcagcgcgcc gcggtcgacc ccggtggggc gcagcagatc ggtgcggagc tcgaggtaga
      181 gcacgatccc gcacgcgatc gtcccgggca gccacgcgat cggatacgcg ccgaccacgc
      241 cgtggacgag cagcacgtcg gtcgcgccga tcgcggccag caccagcgcc gcgaccagca
      301 cccgcagcat tctgcgccgc tcgccgcgca tcatcgaccg ccgcgcgatg atcaggccga
      361 tcgcgatcca catcgcgagc tggccgatgt ggatgtcggt gagcggcccc ccggttgggt
      421 aaaacacgcc ggaggtcagg tgatgtactc ccggcaccgt ccagctggtg ccccagcaca
      481 acccgagcag cgcggtgccg accgccccgg aggccgcgac cacccagcga tggcgctcca
      541 gctgtccgct gatcccgagc aggaccagca agagattcgg gccgatcagc gacaccggcc
      601 cggcgccgag ccggagcagc cggaccgcga cctcggggtc atcggtgcag ctcgccaggg
      661 cggagccgat cgtccacggc agcccggcgg tcgccgcccc gatcatcccg agccgcatca
      721 cccggtcgcc gcggaccagc ccggcgacga ccccgaccgc acacagcgcc gccgcgcatg
      781 cgaggtacgg gacgcacagc cagctccacc ggaggtcgaa cacggccgtg cgacgctagc
      841 acctgtggac gcggacttgg cctgtggcca tcgcacatcg aatagcgcgg tgtgtacaat
      901 gttgtccttg tcgcgccgct cgcctcgcta tcgcatcgat accgcagcca cggcggtccc
      961 gctcctgggg ggcgacatcg ccgcgtgccg cacgcacgac gtctcggagt ccgggctcag
     1021 cctcgacacc gccgcgtggt tcccgctcgg gacccggctc gccgtcgcgc tggtcgatcc
     1081 cacgaccggc agcgcgatcg aggtgatcgg cgacgtcgtc cgggaggccg ccccgccgag
     1141 ctggacgctc ggcatccttc tgatcgatcc gcccctggag tggcaggggg tggtcgctac
     1201 cgcagcgcgg cgctcgggca acgcgccggt caagccgacc aagcggatgc gcgtgctggt
     1261 gttcggcgat gaccaccgcc agcgcggcgc gatggcgctc tacgtgacca gcggctggga
     1321 cgtgctgttt gccaccgacg aggactcgat cgccgaggcg ctggcccaca tcgagctcga
     1381 cgcggtggtc gccgagctcg acgtcaccga tccgcggctg ccgccgatca tgcacagcgt
     1441 gcgccgatcg cagccctccg cccgccggat cgcgcgcggc accgggcgcg ccgagaccga
     1501 gctggtccat cgcttcgtcg atcgcgacgc cggcctcgac gccctgctcg acgcggtgac
     1561 cgccgacatc ccggtcggct gatgcggcgc ggcgatcgat tcacctgggc gtcgccggct
     1621 gcgccggggt gcccgacgac gccggggccc ggccgaacac ccggcgcagc tggttcgagc
     1681 cgacgcgcag cacgccggtg aggaacgcgc gtcgccacga gtggccggtg agctcggcga
     1741 tcagcttgcg cgccgcacgc gggtcgacga acgcgtcggt gatccggcgc ggatcgacga
     1801 ggttctcgaa gatcgcgtcg gcgatccgct gccgggggct gtcggtcacc ccgtcgctgc
     1861 cgcactgcga gatcatcagt agccggccgg ccggcgtcat cggctcgagg aacgtgttgt
     1921 tgagcgtcac ggtcggcgcg ccgtggtcgg cccagaactc ctcgaatgtc cgggtcatcc
     1981 acgccgc
//
LOCUS       DASVOR010001920         6509 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341058,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001920 DASVOR010000000
VERSION     DASVOR010001920.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6509
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341058"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /locus_tag="VF516_35605"
     CDS             complement(<1..821)
                     /locus_tag="VF516_35605"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113129.1"
                     /translation="MLTALGRYPIDQLLARGGMGEVYLGRVVGAHGFSRPVVIKVIRT
                     ELVANERIALMFVDEARIAAGLHHRNIVQIIDFDLFDGGAFLVTEYIPGCDLRTLLHH
                     LRAAPRFEVAVTILAELATGLDAAHEAHDGAGAPLNLVHRDVSPSNVLLGVHGEVKVA
                     DFGVAKARSRSYHTVSGSIKGKAPYMAPEQILGEPLDRRADVFALGVLLFELTTRTRL
                     YSAHANSVAMNHILDGHLPDPAERRPDFPRALTAVVQRALARHPADRYPTARALA"
     gene            complement(1150..1449)
                     /locus_tag="VF516_35610"
                     /pseudo
     CDS             complement(1150..1449)
                     /locus_tag="VF516_35610"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(1468..1701)
                     /locus_tag="VF516_35615"
     CDS             complement(1468..1701)
                     /locus_tag="VF516_35615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113130.1"
                     /translation="MTIRGVLLSLGLVVAPALGCNHRSSPAEVAPAQTSAVAVGATAP
                     DAQLVGTTGGKVALADLLHQHAQTVVVFYRGFW"
     gene            1826..3334
                     /locus_tag="VF516_35620"
     CDS             1826..3334
                     /locus_tag="VF516_35620"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF002868.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0071897 - DNA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX8113131.1"
                     /translation="MLYAAIADTSKQLVETSSRKKKIERLARLLGELAPDERPIAARH
                     LSGDVVHKLGIGYATVGELHGQIAPAAASSLTVLDVDRRFAAIAAMQGAGSSKAKRDA
                     FGAMLAAATAAEQAFLGALVVGELRQGALDALVVDAIAAATQLDPRTVRTAYMLAGDI
                     GAVAAAALGDGAAGLARIGLRVFQPILPMLAQTADTAAEALAGFGGPAALELKLDGFR
                     VQIHKDGERIRVFSRALNDVTAQVPEVVAATAQLPARRLILDGEASAFAPSGRPLPFQ
                     DTMRRQGPLRLTLFDALLVDDQTLLAGPARERFAALGELAPALAVPRLITADAGEANA
                     YFEAAVAQGHEGVMAKALDAPYDAGNRGAAWLKIKKVHRLDLVVLAAEWGSGRRRGWL
                     SNIHLGARDGAGGWVMLGKTFKGMTDEMLAWQTAQFLAREVDRDGHVVHVRPELVAEI
                     AFNDVQRSSRYPGGFALRLARVVRYRDDKTAAEADTIDAVRAIAVRDGVVPE"
     gene            complement(3578..4921)
                     /locus_tag="VF516_35625"
     CDS             complement(3578..4921)
                     /locus_tag="VF516_35625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113132.1"
                     /translation="MGRFTLLCLGFVIVSACGSVKDVGHLPDAPPAGDAGTDAVTRGT
                     VRVTVLDPSNTGAPAVGANVVFLDPDGTLVKRVATDTTGKAQADVLPGASVASVALQN
                     NAYQIQAVLAVRPGDDLILGVKNADTTSIGTFTVSFPAYTVVTPASYTVAGACGTASS
                     FPPPAGGPPPASVTMTFNNSCKLDTMEIVVQANDGNGNPLAVLDKTGVALLLNGSTTL
                     SGAYQSPRNFTASYTNVNSLVTSMSTTRRVPDAFGLSASASASNPTATQVLTLTGAVG
                     SGARVETQLISGSRSIQLVRQTIAGTAASYGLDLGATLLPWLGTPTFDVASGKILLPT
                     DTTGTTTATPDLLELLMSYQRTDPVTGITTTFSWTIFSPVAGDITLPSLPPEAGNVMP
                     TASDAVTARAVMVESDGIANYDAIRNDLNAALATYAGNRAPGTTLRAAFSPLNLR"
     gene            complement(4992..6017)
                     /locus_tag="VF516_35630"
     CDS             complement(4992..6017)
                     /locus_tag="VF516_35630"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF006850.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006146 - adenine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEX8113133.1"
                     /translation="MTAIDPARDRLIRRLPKVELHLHIEGTLEPEMMFSLAAKHGIPL
                     PYRSVADVRAAYQFSDLQSFLDLYYAGCSVLRDRRDFYALAMAYFTRAHADHVVHAEL
                     FFDPQTHTVRGIALDAVFGGLRDAMEDALRRYGISSELILCFLRHLSEDDAQATLDAA
                     LPFRREFIGVGLDSGERGNPPSKFERVFARARSLGLHAVAHAGEEGPAGYVREALDLL
                     RVERIDHGVRCDEDPALVDRLVRDGVPLTVCPLSNLKLCVVSDLRAHNFARLLRRGVA
                     VTINSDDPAYFGGYIADNYRATAAALELTAPELVAVAENAVRASFLPATAKAALLGRI
                     RQASVRA"
     gene            6036..>6509
                     /locus_tag="VF516_35635"
     CDS             6036..>6509
                     /locus_tag="VF516_35635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tex-like N-terminal domain-containing protein"
                     /protein_id="HEX8113134.1"
                     /translation="MPLVEDVGTVANPMTDDATPEPTAASAPPHYDPVPAVARELGLA
                     PAAVAAVVAMLDEGNTVPFIARYRKERTGGLDEVQIRAIEDQRTYVVELEARRAAILA
                     SVAEQGKLTPELEAKLRAARGKSELEDLYAPYRPRRKTRASVARERGLEPLAQRIL"
BASE COUNT          955 a         2260 c         2376 g          918 t
ORIGIN      
        1 tcggcgagcg cccgcgccgt cgggtagcgg tcggccggat ggcgcgccag ggcgcgctgc
       61 acgacggcgg tcagcgcgcg cgggaagtcc gggcgccgct ccgccggatc gggcaggtgg
      121 ccgtcgagga tgtggttcat cgcgacgctg ttggcgtgcg ccgagtacag ccgggtgcgg
      181 gtcgtcagct cgaacagcag cacgccgagc gcgaacacgt cggcgcggcg atcgagcggc
      241 tcgccgagga tctgctcggg ggccatgtac ggcgccttgc ctttgatcga gcccgacacc
      301 gtgtgatagc tgcgcgaccg cgccttggcg acgccgaagt ctgcgacctt gacctcgcca
      361 tgaacgccga gcaagacgtt gctcggactc acgtcgcggt gcaccaggtt aagcggcgcg
      421 ccggcgccgt cgtgcgcctc gtgcgccgcg tcgagcccgg tcgccagctc ggcgaggatc
      481 gtcaccgcga cctcgaaccg cggcgccgcg cgcaggtggt ggagcagcgt gcgcagatca
      541 cagccgggga tgtactcggt gaccaggaac gcgccgccgt cgaacagatc gaagtcgatg
      601 atctgcacga tgttgcggtg gtgcaggccc gccgcgatcc gggcctcgtc cacgaacatc
      661 agcgcgatcc gctcgttggc gaccagctcg gtgcggatca ccttgatcac gaccggccgg
      721 gagaacccgt gcgccccgac cacccggccg aggtagacct cgcccatgcc gccgcgcgcc
      781 agcagctggt cgatcgggta tctgccgagc gcggtcagca cgacgggggg cgagcgaagc
      841 gagacggggg accccgacgg cgctgccggc ggggcgggga gcagagcctc ccgtgggttc
      901 gatgatgcca cggcagcgac ggccactggg cctgccaccg cccatggcag atggagttgc
      961 aggcagcgga ttcatgcccg cgatcaccag tcatgtcggt gacttcgcct cgggccgaca
     1021 tggcatggat cgcgcacaca tggcgatcat ctcgaggccc ttgcatcgca agcacctcga
     1081 ccctggcgcc cgccgcggcg ccaggtcgcg ttttcggctt cacgatcact tcatgcgctt
     1141 cgtgcgcgct cagtgcagcg ctgcggcgag ctcggcgtag gtcggccagt cgccgcgctt
     1201 gtcctccagg tggcgccact gcaccacgcc gtcgcgtccg accacgaacg tccccgggct
     1261 cgggctctcg gcgccgggga cgtgcacgcc ccacgcggtc gacgcggcga cgccgggatc
     1321 ggtgagcagc gtcacgccgg gcgccttggc ctgcagcgcc gccatgtcgg agacactgtc
     1381 ggcggcgatc gcgacgatcg cgatgtcgcg gtgcccgagc tcgtccgcgt gcttggccag
     1441 ctcaccgagc cagacgacgc agctgtgtca ccagaacccg cggtagaaca cgaccacggt
     1501 ctgcgcgtgc tggtgcagca gatcggcgag cgcgaccttg ccgcccgtcg tcccgaccag
     1561 ctgtgcgtcg ggagccgtcg caccgaccgc gaccgcgctc gtctgcgccg gcgccacctc
     1621 cgcgggcgat gaccggtgat tgcagcccag cgccggggcc accaccagtc ccagcgagag
     1681 caggacgccg cggatcgtca tggcgagaaa gctacgcgac ccgcggcgct gccgccagcg
     1741 atcggcccgg acttcccgca cggcgggcgg acacggcgtt tgcggggcgg gcgacgccgc
     1801 gaggttcccc cggtaggctt gcgccgtgct gtacgcggcg atcgcagaca ccagcaagca
     1861 gctggtcgag acctcctcgc gcaagaagaa gatcgagcgg ctcgcccggc tgctcggcga
     1921 gctcgcgccc gacgagcgtc cgatcgccgc gcgccacctg tccggcgacg tcgtccacaa
     1981 gctcgggatc ggctacgcca cggtcggcga gctgcacggc cagatcgcgc cggcggcggc
     2041 gtcgtcgctc accgtgctcg acgtcgatcg ccggttcgcc gcgatcgccg cgatgcaggg
     2101 cgcggggtcg agcaaggcca agcgcgacgc gttcggcgcg atgctcgccg cggcgaccgc
     2161 cgccgagcag gcattcctcg gtgcgctcgt ggtcggcgag ctccgccagg gcgcgctcga
     2221 cgcgctggtg gtcgacgcga tcgccgctgc gacccagctc gacccgcgca ccgtgcgcac
     2281 cgcgtacatg ctcgccggcg acatcggcgc ggtcgccgcc gccgcgctcg gggacggcgc
     2341 cgccggcctg gcgcggatcg gcctgcgcgt gttccagccg atcctgccga tgctcgccca
     2401 gaccgcagac accgcggccg aggcgctcgc cggcttcggc ggtccggcgg cgctcgagct
     2461 caagctcgac ggcttccggg tccagatcca caaggacggc gagcgcatcc gcgtgttctc
     2521 gcgcgcgctc aacgacgtca ccgcgcaggt ccccgaggtc gtcgccgcga ccgcccagct
     2581 ccccgcccgc cggctgatcc tcgacggcga ggccagcgcg ttcgcgccga gcggccggcc
     2641 gctgccgttc caggacacga tgcggcgcca gggcccgctc cggctcaccc tgttcgatgc
     2701 cctgctggtc gacgatcaga cgctgctcgc cggccccgcg cgcgagcggt tcgccgcgct
     2761 cggcgagctc gccccggcgc tcgccgtgcc ccggctgatc accgccgacg ccggcgaggc
     2821 caacgcctac ttcgaggccg cggtcgccca gggccacgag ggcgtgatgg ccaaggcgct
     2881 cgacgcgccg tacgacgccg gcaaccgcgg cgccgcgtgg ctcaagatca agaaggtcca
     2941 tcgcctcgat ctcgtcgtgc tcgccgccga gtggggctcg ggccgccgcc gcggctggct
     3001 gtccaacatc cacctcggcg cgcgcgacgg cgccggcggc tgggtcatgc tgggcaagac
     3061 gttcaagggc atgaccgacg agatgctggc ctggcagacc gcgcagttcc tcgcccgcga
     3121 ggtcgatcgc gacggccacg tcgtccacgt ccgccccgag ctggtcgccg agatcgcgtt
     3181 caacgacgtc cagcgctcgt cgcgctaccc cggcggattc gcgctccggc tcgcccgcgt
     3241 cgtgcgctat cgcgacgaca agaccgcggc cgaggccgac acgatcgacg ccgtgcgggc
     3301 gatcgccgtc cgcgacggtg tggtcccgga gtgaccggcc agctcatgga cgcgcgtgcg
     3361 acaggatccg gaaccgcatg cacgtactac gagctcgcca ggaacgggcg atatcgcggt
     3421 acaccgccgt ccgcggtcgc cgcgctccga tgctggccct tgaacgacct tcggagggga
     3481 atggctgagt tgctccaact gaactgtcgc acttcacgcc agcactggca ctgaccatgc
     3541 aaactcttcg tcgaaagagc tatcgtatgt cgtggtgtca gcgcaggttc agcggcgaga
     3601 acgccgctcg cagcgtggtt cccggtgccc ggttgccggc gtacgtggca agcgccgcgt
     3661 tcaggtcgtt tcggatcgcg tcatagttcg cgatgccgtc gctctccacc atcacagcgc
     3721 gtgcggtgac cgcgtcggac gccgtcggca tcacgtttcc ggcctccggt ggaaggctcg
     3781 gcaaggtgat gtcgccagcg accggcgaga agatcgtcca ggagaacgtc gtcgtgatgc
     3841 cggtcaccgg atcggtgcgc tggtagctca tgagcaactc gaggagatcc ggcgttgcgg
     3901 tggtagttcc cgtcgtgtcg gtcggcaaca ggatcttgcc gctggcaaca tcgaacgtgg
     3961 gcgtgccgag ccacggcagc agcgtcgcgc cgaggtcgag cccgtacgac gcggcggttc
     4021 ccgcgatcgt ctgacgcacg agctggatcg accgcgaccc gctgatgagc tgggtctcga
     4081 cccgcgcacc gctgccgacc gcaccggtga gggtaagcac ctgggtcgcg gtcgggttcg
     4141 acgccgaggc gctggccgac aggccgaacg cgtccgggac cctgcgcgtc gtcgacatgc
     4201 tcgtgacgag cgagttgacg ttggtgtacg aggcggtgaa gttgcgcggc gactggtacg
     4261 cgccggacag cgtcgtactg ccgttcagca ggagagcgac gccggtcttg tcgagcacgg
     4321 cgagcgggtt cccgttccca tcgttggcct gcacgacgat ctccatcgtg tccagcttgc
     4381 aggagttgtt gaacgtcatt gttaccgacg ccggcggcgg gccaccggcc ggcggtggga
     4441 acgatgaggc agtgccgcac gcgcccgcca ccgtgtagct ggcaggggtg accacggtgt
     4501 acgctgggaa cgacactgtg aacgtaccga tggaggtggt gtcggcgttc ttgacgccga
     4561 ggatcaggtc gtcgccgggc cggaccgcga gcacggcctg gatctgatac gcgttgttct
     4621 gcagcgcgac cgacgccacg ctggcgccag gcagcacgtc ggcttgggcc ttgccggtgg
     4681 tgtcagtcgc gacgcgcttg acgagtgtgc cgtcgggatc gaggaacacc acgttggcgc
     4741 cgaccgccgg cgcgccggtg ttgctcgggt caagcacggt gacgcgaacg gtcccgcgag
     4801 tcacagcgtc ggtgccggca tccccggcag gcggggcatc cggcaggtgc cccacgtcct
     4861 tgacgctacc gcaggccgaa acaatgacga aaccaaggca taagagagtg aaacgaccca
     4921 tcctcggcat gatacggtag ccgatgctgg ttcttctcca aaagaactgc gatctgcctc
     4981 acagttgtgg gtcacgcacg caccgaggcc tggcggatcc ggcccagtaa cgcggccttg
     5041 gccgtcgcgg gcaggaacga cgctcgcacg gcgttctcgg ccacggcgac cagctcggga
     5101 gcggtgagct ccagggcggc ggcggtggcg cggtagttgt cggcgatgta gccgccgaag
     5161 taggccgggt cgtcggagtt gatcgtgacc gcgacgccgc ggcgcagcag gcgcgcgaag
     5221 ttgtgggcgc gcagatcgga cacgacgcac agcttgaggt tggacagcgg gcacacggtg
     5281 agcggcacgc cgtcgcggac caggcggtcg accagcgcgg gatcctcgtc gcagcgcacg
     5341 ccgtggtcga tgcgctcgac gcgcagcaga tcgagcgcct cgcggacgta gccggccggg
     5401 ccctcctcgc cggcgtgggc gaccgcgtgg aggcccaggc tgcgcgcgcg ggcgaacacc
     5461 cgctcgaact tgctcggtgg gttgccgcgc tcgccggagt cgaggccgac gccgatgaac
     5521 tcgcggcgga acggcagggc ggcgtcgagg gtggcctgcg cgtcgtcctc ggacaggtgg
     5581 cgcaggaagc acaggatcag ctcggagctg atgccgtagc ggcgcagggc gtcctccatc
     5641 gcatcgcgca gcccgccgaa cacggcgtcg agtgcgatgc cgcgcacggt gtgggtctgg
     5701 gggtcgaaga acagctcggc gtggacgacg tggtcggcgt gggcgcgcgt gaagtaggcc
     5761 atcgcgagcg cgtagaagtc gcggcggtcg cgcagcacgc tgcagccggc gtaatagagg
     5821 tcgaggaacg actggaggtc ggagaactgg taggcggcgc ggacatcggc caccgagcgg
     5881 tagggcagcg ggatgccgtg ttttgccgcg aggctgaaca tcatctcggg ttcgagcgtg
     5941 ccctcgatgt ggaggtggag ctcgaccttg gggaggcggc ggatcaggcg gtcgcgggcg
     6001 gggtcgattg cggtcacggt gggctacctt actacttgcc gctcgtcgag gacgtgggta
     6061 cggtcgccaa cccgatgacc gacgacgcca cccctgagcc caccgcggcc tccgcgccgc
     6121 cccattacga cccggtcccg gccgtggcgc gagagctcgg gctggcgccc gcggccgtcg
     6181 ccgcggtggt cgcgatgctc gacgagggca acacggtgcc gttcatcgcg cgctaccgca
     6241 aggagcggac cggcgggctc gacgaggtcc agatccgcgc gatcgaggac cagcggacct
     6301 acgtcgtcga gctcgaggcc cggcgcgcgg cgatcctggc gtcggtcgcc gagcagggca
     6361 agctcacgcc cgagctcgag gccaagctgc gggcggcgcg cggcaagtcc gagctcgagg
     6421 acctgtacgc cccgtaccgg ccgcgccgca agacccgggc gtcggtcgcg cgcgagcgcg
     6481 ggctcgagcc gctggcgcag cggatcctc
//
LOCUS       DASVOR010001921         2054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_399295,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001921 DASVOR010000000
VERSION     DASVOR010001921.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399295"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            603..1589
                     /locus_tag="VF516_35640"
     CDS             603..1589
                     /locus_tag="VF516_35640"
                     /inference="COORDINATES: protein motif:HMM:NF020448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1835 domain-containing protein"
                     /protein_id="HEX8113135.1"
                     /translation="MASTLTEAIHLVSSASAAGLVIHVGVPRNHVRILSERLTIGPCD
                     VDPERHVELRRAWDAEALSETFGIDDLRAAVAGELPVVIWATRAYDDLVWLWWILDGL
                     ARLHRPPFVARPVLADPLETVAGATPKEGRAALRVARALRDDEIREGCELWRLYASPD
                     PLAFDEARRKGSQAFPELSESAELLGTWFPRLDGGRLRLSEHDERLLGSLTDEWLMPL
                     DLSKLITKTRGWERLLWPFGGFVSIWRLRQWAAHGAVAREVRDPNNDNPLERDAFRLT
                     DKARALLVKGLEGVGDAPPVYVGGCRLNDPAAPWVRVAEDSGWRIIRLTVRT"
     gene            complement(1573..>2054)
                     /locus_tag="VF516_35645"
     CDS             complement(1573..>2054)
                     /locus_tag="VF516_35645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113136.1"
                     /translation="GTIDEKDAIFSGLRLWQDKNHDGVSQPAELHTLPELGVAGISLG
                     YVEAREVDQHGNSFRYRSTVYGTPGSPVGMTAWDVWLTGIRPEPVVSFRADGHGNPAE
                     LKRCYMAADGDYDTWIAYCKTVPGSIRASCYEIGHQSRQRKRGWCDLYWGRVLTCGQ"
BASE COUNT          336 a          656 c          688 g          374 t
ORIGIN      
        1 ggccggagtc tgtgccgcgg tcgcgccggc cagcgcacac acgagcacaa gcgacatccc
       61 tggacggtgc atggcatggt tgtgccagac ctcgggaccc gttcgggaaa cttctgtgca
      121 caatcgctgg catcatgcgg caaaccggcc gcggtcggcg ccgcagtcgg gccagtccgt
      181 agggctggcg caggactgca ttgcagggag ggggcggccc atgcccccac gtggggtcgc
      241 tcgggtccgg gatctcgacg ccgggctgct gcgcgaaccg gcggacgtcc gcatcggtgg
      301 ggccgtcggc ccgcgttgtt tttatcgggc ccagcgcgac ggtgacgccc gccttcgcga
      361 accgcccgac gtgcagcgcc gacttggccg ccgcgcggcc tccgcagcga gcggtcctca
      421 cctcgtcgag atggcggact cggcgaggtc ggcggcgacg cgagctggcg cagcggcacg
      481 aacgcacgtg agcgccggca agttaattcg tcgacgccga cgatcgcaat acagttcgct
      541 ccgatgcccg aaattccaag ctacggctcg cccgccagca gccaaggggt aggctccgct
      601 gcatggcatc gacccttacc gaggcgattc atcttgtgtc cagcgcttcg gcagccgggc
      661 tcgtcataca tgttggggtg ccaaggaacc acgtccgcat cttgagcgag cggctcacga
      721 tcggtccctg cgacgttgat ccggaacgtc acgtcgagtt gcgccgtgca tgggatgccg
      781 aggcgttgtc cgagacgttc ggtatcgacg acctgcgcgc agccgtagca ggcgagttgc
      841 ccgtcgtcat ctgggcgacg cgggcgtatg acgatctcgt ctggctctgg tggattctgg
      901 acgggcttgc tcggctccat cggccgccgt tcgtggcacg accggtgctc gccgacccgc
      961 tcgagaccgt agccggggcg actcccaagg agggtcgcgc ggcactcagg gttgcgcgcg
     1021 ccctgcgcga cgatgagatc agggaaggct gcgagctctg gaggctgtac gcgtcgccgg
     1081 atccgctcgc cttcgacgag gcgcgcagga aaggctcgca ggcgttcccg gaactcagcg
     1141 agagcgccga gctccttggg acatggttcc cgaggctcga cggcggccgg cttcgtctgt
     1201 ccgagcatga cgagcgcttg ctggggagcc tcacggacga gtggttgatg ccgttggatc
     1261 tgagcaagct gatcactaag acgcgtggat gggagcggct gctgtggccg ttcggtggat
     1321 tcgtttcgat ctggaggcta cgacagtggg ccgcgcacgg cgcggtcgca cgcgaggttc
     1381 gcgaccctaa caatgacaat ccactcgagc gggatgcatt tcgcctcacc gacaaagcgc
     1441 gcgccttgct ggtgaagggg ctcgaagggg ttggcgacgc accaccggtc tacgtgggcg
     1501 gctgccgact gaatgatcca gcggcgccat gggttcgcgt cgccgaggat tcgggttggc
     1561 gaattatccg cctcactgtc cgcacgtgag gaccctgccc cagtataaat cacaccatcc
     1621 tcgctttctc tgtctgctct gatgtccaat ttcataacaa cttgcacgaa tgctcccggg
     1681 cactgtttta cagtaagcga tccaggtgtc gtaatcaccg tcagctgcca tatagcatct
     1741 cttgagctcc gctggattgc cgtggccatc ggctcggaag ctgacgacag gttcgggccg
     1801 gatgccggtg agccagacgt cccaggccgt catcccaacg ggagacccgg gcgttccata
     1861 caccgtggac ctgtaccgga acgagttgcc gtgctggtct acctcacggg cttcgacata
     1921 gcccagcgag attccagcaa ccccgagttc aggcagcgtg tgcagttccg cgggctgtga
     1981 taccccatcg tggttcttgt cctgccagag acgcagcccc gagaagatcg cgtccttctc
     2041 gtcgatcgtg ccat
//
LOCUS       DASVOR010001922         1530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_165989,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001922 DASVOR010000000
VERSION     DASVOR010001922.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1530
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165989"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="VF516_35650"
     CDS             <1..748
                     /locus_tag="VF516_35650"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEX8113137.1"
                     /translation="ELFAKDADEVRPIASTTKIFVAMAVRKHLDLDGWTEITKVDAHY
                     AAGGARTRLDIGQKFRNKDLLRAMLIASDNRAPTALGRAAGMSPDDLVAAMNEIAKHL
                     HLKHTRFTDPTGLHGNVSTAREMAIALRTVLADEVLRAVLRQDSADVVSKDHYARIHY
                     GTTNAPLVVGKWDVIGGKTGYTHAAGYCFVTGARFDDREVVMAFLGASGKLTRFADFN
                     RVAAWLDHRRGPATADATADPASDSGDDDR"
     gene            complement(745..1269)
                     /locus_tag="VF516_35655"
     CDS             complement(745..1269)
                     /locus_tag="VF516_35655"
                     /inference="COORDINATES: protein motif:HMM:NF027567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8113138.1"
                     /translation="MGLSTATIEMQSPEEHRERLHELVKAARTVLVLHCDRDDRIVGQ
                     PTALLRTGDDTTLYVAASLDADQRAALSRPARVTVVVPDGSHAMFDAEAVISRDRTLL
                     DLTGGAAPRLALLDEGGEASKAGRSKDPSVAVLVISPIEGAYWQGPRRHAYQYRVAPA
                     RGTRELSDGVPVEI"
BASE COUNT          235 a          620 c          469 g          206 t
ORIGIN      
        1 cgagctgttc gccaaggatg ccgacgaggt ccgcccgatc gcgtccacca ccaagatctt
       61 cgtcgccatg gcggtgcgca agcacctcga cctcgacggc tggaccgaga tcaccaaggt
      121 cgacgcccac tacgccgccg gcggcgcgcg cacccgcctc gacatcggcc aaaagttccg
      181 taataaagac ctgttacgcg cgatgctgat cgcgagcgac aaccgcgccc cgaccgcgct
      241 cggccgcgcc gccggaatgt ccccggacga cctcgtcgcc gccatgaacg agatcgccaa
      301 gcacctccac ctcaagcaca cccggttcac cgacccgacc ggcctacacg gcaacgtgtc
      361 caccgcgcgc gagatggcga tcgcgctgcg caccgtgctc gccgacgagg tcctgcgcgc
      421 cgtcctgcgc caggactccg ccgacgtggt gtccaaggac cactacgcca ggatccacta
      481 cggcaccacc aatgcgcccc tcgtcgtcgg caagtgggac gtgatcggcg gcaagaccgg
      541 ctacacccac gccgcgggct actgcttcgt caccggcgcg cggttcgacg atcgcgaggt
      601 cgtcatggcg ttcctcggcg ccagcggcaa gctcacccgg ttcgccgact tcaaccgcgt
      661 cgccgcgtgg ctcgaccacc gccgcggccc ggcgaccgcc gacgccaccg cggatccggc
      721 ttccgattcc ggcgacgacg atcgctagat ctccaccggc acgccgtcgg acagctcgcg
      781 cgtcccgcgc gccggcgcca cccggtactg gtacgcgtgc cgccgcgggc cctgccagta
      841 cgccccttcg atcggcgaga tcaccagcac cgcgaccgac ggatccttgc tccgccccgc
      901 cttcgacgcc tcgccgccct cgtcgagcaa agcgagacgg ggggcagcgc cccccgtgag
      961 atcgagcagc gtgcgatccc gcgagatcac cgcctcggcg tcgaacatcg cgtggctgcc
     1021 gtccggcacc accaccgtca cccgtgccgg gcgcgacagc gccgcccgct gatcggcgtc
     1081 gagcgacgcc gccacgtaca gcgtcgtgtc gtcgcccgtg cgcagcagcg ccgtcggttg
     1141 cccgacgatc cgatcgtcgc ggtcgcagtg cagcaccagc accgtccgcg ccgccttcac
     1201 cagctcgtgc agccgctcgc ggtgctcctc gggactctgc atctcgatgg tcgccgtgct
     1261 cagccccatg taaggaagcc aaagcaaatc acatgcctcg atcgcggcac tggcgcacgc
     1321 tgcgtgacgg acgcgcccgc gcccacttac cccgccgcgg cgctcgcgtg cggtgtgcgc
     1381 cctcgctcca gcgcccgccg acgccgtggc atgctcgcgc cgtgaccctc caccgacgcg
     1441 aggtgccgct gatctgcgcg agcggttcac cgcctggctg gcgcagtacg agactgagag
     1501 catggggcgg ggatattcct ccccgccccc
//
LOCUS       DASVOR010001923         1680 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_428548,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001923 DASVOR010000000
VERSION     DASVOR010001923.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428548"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..877
                     /gene="cobA"
                     /locus_tag="VF516_35660"
     CDS             68..877
                     /gene="cobA"
                     /locus_tag="VF516_35660"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEX8113139.1"
                     /translation="MTGFVSLVGAGPGDPELLTLRALRRLREADLVLNDALVHTDLLE
                     LAPKAQRFYVGKRAGRHSIDQDGIHRLMIRAARRGQRVVRLKAGDPFVLGRGGEEALA
                     LEAAGIAYEVVPGLSSAIAAPLLAGIPVTHRGLASGFAVVSGHAESAYAPILDSIAPG
                     AMTLVVLMGVKARGAIAGRLIARGWPDTTPAALLFGASQPGGFTRLTTLGALVASGGR
                     ESPLSIDDIATELPGVLVIGEVVSLAGAIESVSQLPSAPLAQPAPHLTRSL"
     gene            874..>1680
                     /locus_tag="VF516_35665"
     CDS             874..>1680
                     /locus_tag="VF516_35665"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF013259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HEX8113140.1"
                     /translation="MSVIDVPPPVAPGRLGFARKADVDLFVQRLEAYERGEISPDEWR
                     GFRLLNGVYGQRQDGLMMIRAKLPGGFLTPAQLEALADVAETYGGGKGHVTTRQNVQY
                     HFVPMADTEAALSRLAASGITTKEACGNSVRNWTCCPFAGVARDEPFDPTPYVEALAR
                     HLLRGPYSSSLPRKLKPSVGGCCGTDCSQAFINDLGFLARARTAPDGSVERGFQLLAG
                     GGLSTLRRSALTVEEFVPAGEILEAADAVVRVFHRIGNRHNRAKARLKWAI"
BASE COUNT          202 a          664 c          577 g          237 t
ORIGIN      
        1 tcggcgtgct ctaccgcagc gatcagtccg ccgagccgcc cgagctcgag ttgccggagg
       61 tcgcgtcgtg accggcttcg tgtcgctggt cggcgccggt cccggcgatc ccgagctgct
      121 cacgctgcgc gcgctgcgcc ggctgcgcga ggccgacctc gtgctcaacg acgcgctggt
      181 gcacaccgac ctgctcgagc tcgcgcccaa ggcccagcgg ttctacgtcg gcaagcgcgc
      241 cggccggcac tcgatcgacc aggacggcat tcaccgcctg atgatccgcg ccgcgcggcg
      301 cggccagcgc gtggtccggc tcaaggccgg cgacccgttc gtgctcggcc gcggcggcga
      361 ggaagcgctc gcgctcgagg ccgccggcat cgcctacgag gtcgtgcccg gcctgagctc
      421 ggcgatcgcc gcgccgctgc tcgccggcat cccggtcacc caccgcggcc tcgcgtcggg
      481 cttcgcggtg gtgtccggcc acgccgagtc cgcctacgcc ccgatcctcg acagcatcgc
      541 gcccggcgcg atgaccctgg tcgtgctgat gggcgtcaag gcgcgcggcg cgatcgccgg
      601 ccggctgatc gcgcgcggct ggcccgacac cacaccggcc gcgctgctgt tcggcgcgtc
      661 ccagcccggc ggcttcaccc ggctcaccac gctcggcgcg ctggtcgcct cgggcgggcg
      721 ggaaagcccc ctgtcgatcg acgacatcgc caccgagctg cccggcgtgc tggtgatcgg
      781 cgaagtcgtc tcgctcgccg gcgcgatcga atcggtctcc cagctgccct ccgccccgct
      841 tgcccagccc gccccccatc tcacgaggtc gctatgagcg tcatcgatgt ccctcccccc
      901 gtcgcccccg gccgcctggg gttcgcccgc aaggccgacg tcgatctgtt cgtccagcgc
      961 ctcgaggcct acgagcgcgg cgagatctcg cccgacgagt ggcgcggctt ccgcctgctc
     1021 aacggggtct acggccagcg ccaggacggc ctgatgatga tccgcgccaa gctgccgggc
     1081 ggctttctca ccccggcgca gctcgaggcg ctcgccgacg tcgccgagac gtacggcggt
     1141 ggcaagggcc acgtcaccac ccggcagaac gtccagtacc acttcgtgcc gatggccgac
     1201 accgaggccg cgctgagccg gctcgccgcg tccggcatca cgaccaagga ggcgtgcggc
     1261 aactcggtgc gcaactggac gtgctgtccg ttcgccggcg tcgccaggga cgagccgttc
     1321 gacccgacgc cgtatgtcga ggccctggcg cgccacctgt tgcgcgggcc gtactcgtcg
     1381 agcctgccgc gcaagctcaa gccgtcggtc ggcggctgct gcggcaccga ctgctcgcag
     1441 gcgttcatca acgacctcgg gttcctcgcc cgcgcgcgga ccgcgcccga cggcagcgtg
     1501 gagcgcggct tccagctgct cgccggcggc gggctgtcga ccctgcggcg cagcgcgctc
     1561 accgtcgagg agttcgtgcc ggccggcgag atcctcgagg ccgccgacgc cgtggtccgc
     1621 gtgttccacc ggatcggcaa ccgccacaac cgcgccaagg cgcgcctcaa gtgggcgatc
//
LOCUS       DASVOR010001924        11288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_224775,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001924 DASVOR010000000
VERSION     DASVOR010001924.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11288
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224775"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VF516_35670"
     CDS             complement(<1..197)
                     /locus_tag="VF516_35670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113141.1"
                     /translation="MHELVTGARDVGRGLAVLRAHPALWKWVVAPAAVTLVLVVAAVV
                     GVVHAVGPVVDWAAAHLPGPLA"
     gene            307..2064
                     /locus_tag="VF516_35675"
     CDS             307..2064
                     /locus_tag="VF516_35675"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8113142.1"
                     /translation="MAERCRRCEEELSTKARFCSNCGFPVSAANPGAVTEDATATEPG
                     QHRLPPRLIAAGTRITDRYMIDGVLGEGGMGVVYRANDLARERSVAIKALHASLMGDA
                     EIRRRFTREAQLMLGWNHRHVARVHEIIEHPDLLAFVMEYVEGPTLEEHAQRWNGKLP
                     YDEIRVIFSGVLDAMREAHAAGIVHRDLKPHNVLLRMEDDGVIPKIVDFGIAKILEGT
                     TYTMTGALLGTCRYMSPEQAQSPQDVDHRTDIYSLGVTLYRCVTGRCPFEGNNHFAVM
                     MAHVSQPPEPPSAFRPHMPPALEQIILRALAKDRAERPQSCAEFRAALEAALVDVTPS
                     RVDRDTEYPRIIIEDDDNEMRLIPAGSFQLGAHRRTVVLDRFYIARYPVTNRQFQAFV
                     EATGYKPTDAEAHRFLQHFRNGRCPPELLNHPVVFVSWTDARAYCRWAARRLPSEAEW
                     EKAARGPDGYKYPWGRDEPTAEHANFGQARAKHYPLGTGGTGGTAPVDAFPRSASPYG
                     IEGMAGNVFEWCEDVDDPGFYLHGPERNPRNTIQPGNAPCVIRGGSWRYDVRSLRTYA
                     RASFAPTFRLDTVGFRVAL"
     gene            2089..3357
                     /locus_tag="VF516_35680"
     CDS             2089..3357
                     /locus_tag="VF516_35680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113143.1"
                     /translation="MEASMPHVTRRIGLSLGADICWPICYEEIVKRLNLAIPWQGDTV
                     GIDVERVTIEPFDLRQPCRYDLVIDRLTHWYHTSREWIKKAVIMDGLYVFNNPWSVQS
                     NEKQTTYCAMMHLGMPIPDTWLIPPKSYEPLPDLQPTLRSYAKLFDLGAVGKQLGYPL
                     FMKPYDGGGWKGVSKIDDEAALRARYEESGKLVMHLQKAVHPFDSFVRCIGLGPQTYL
                     VKYDPGAPLHDRYTMAKGFVPADEAAVLRDTTLTINAFFGWDFNSCEALRKDGTWYPI
                     DFANPCPDSQVTSLHYHFPWLVKANIRWSVFNAVTRRPFRRTLDWEPFYAIARRDLPY
                     RERLREYARVAEQRFDAAAFEEFCARHLAHLDEVAWEFFATPTARDAVKKKVAALFPA
                     HEVELFTELFWRRIGAWRDDHKPGATGAVP"
     gene            3354..4100
                     /locus_tag="VF516_35685"
     CDS             3354..4100
                     /locus_tag="VF516_35685"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEX8113144.1"
                     /translation="MTLDDVKTIGRWKSERLGREVTMVRWGSFGQPVLVFPTAGGDAE
                     EIERMHLVGALGPLIEAGQIKVYSCDSVAGRALLTREGSPQHQMWLQDQFHHHVRHEV
                     VPAIHADCKSELDVWAVGASIGAFHSVAVVCRFPDVFARACAMSGTYDLRRFYEASEF
                     TDAFWASSPLHFVPRLSGHHLDVLKTRMIVLASGEGRAEDLGESWRMANALGAQGIPN
                     RVDPWGPGWHHDWPTWREMLPKYLGEWVKE"
     gene            complement(4111..6504)
                     /locus_tag="VF516_35690"
     CDS             complement(4111..6504)
                     /locus_tag="VF516_35690"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113145.1"
                     /translation="MGHTASFQGIGGLGKTQLAVEYADRYGATYAGGVLWLDADRDIE
                     AQLVKISDEARWVDPRSEHQAKLDVALHRLHTHTGCLIVFDNVNDPAAIERYLPVPAA
                     AAHILVTSRAEQPGFDPIALDVLDTAQSLELLVQEAGRAPVNGDERTAAQAIAARLAG
                     LPLALEIAGAYLRHRAPMPWRDYQRLLDDSLKQALSGSFLQSFTTHDKDLYATLRVTE
                     AMLGDEPRLRDVLDVLTVSGSASMSTSLLAALLDVSETALIGPLSLAVKLRLLGIEDD
                     PVGGGAQRYRLHRLVQEVRRDEIAIEHRTAWAQDVSRRLAAWFEHRRVEFADLPAFEA
                     EIDHLRAWQRHALDGCWPGRARLAWLQGYPHYHRGRYREAHEWVKAAERYHRETPAGD
                     DLEALLASDMGALERRLGNPRDALAYYQRALELHRQRHGDGHADTARSLRHVAMAYRG
                     LCDYKQALKLSEQALAMSLAVHGPRHRAIASVLNDVGVTHADLGDLRKALDFHSRALQ
                     LEEELLGAGHPDLAILLGNVGAAYSDLGNHQTALTFIRQAFEIEQKRFGDEHPTTATS
                     RSNLGLTYLWLGEHRLAKEHAEGALRTRREMLGDDHPDTALSLMNVAAVYAALGDHAK
                     ALELREQALHVHARVFGESHPETARALDNLGGTHNDLGDHETALVLAKRSLDIRRSVL
                     GDDHPDTAISFANVSRVETCLGHHKLALEHAQRALDIFAKVHGPGNSMTLDRMLVVVD
                     RWLRYKRPNAAYYLLESWLGKLPPDHPRRAALTARLRELPAPGRRKGSTTRKRSRKR"
     gene            complement(7010..7303)
                     /locus_tag="VF516_35695"
     CDS             complement(7010..7303)
                     /locus_tag="VF516_35695"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8113146.1"
                     /translation="MIARIVEVLHPEQVWLFGSRARGEARADSDWDFMAILADDAPEQ
                     DLDLASVWQRLRDLRLRRVEVFTMTRSEFDEWKRSLGTLAEIVASTGVVVYGG"
     gene            7802..9004
                     /locus_tag="VF516_35700"
     CDS             7802..9004
                     /locus_tag="VF516_35700"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8113147.1"
                     /translation="MALCAVTTPALAQSTIKIAITGPFTGGSAPMGTSARDGAKLAIA
                     EINAAGGIQVGAKKLKIEAIERDDEAKNERGALIGQELAAMSDLSGVIGTVNTGVVLA
                     GDKYLQDKGITRIITPAAGSASMTQWAKPDGPKDLFMFRFAAHDGIQAAMVVEEAIAR
                     KFTKVAIFADATNYGVSGRDDLLDQIKKQGGKLQVVTIEKWAIGDKDMTAQLLKAKAL
                     GAQALLIWGIGPELAAIANGMAKLGYRVPMIGGWALSMSNFLDNAGANGDGAIMPQTF
                     VEEPVTPRAKAFIEAYHKAYNTTRIPSPVSAAQGYDAVLLFAAAVKQAGTTDTKAIRD
                     ALENLKEPVDGVIAVWTKPYSKWNPADVTTHEAFRREQVVMGLVKGGHVTFANETDRQ
                     RLAKRGAK"
     gene            9014..9892
                     /locus_tag="VF516_35705"
     CDS             9014..9892
                     /locus_tag="VF516_35705"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8113148.1"
                     /translation="MPLSVVVQIAVSGALMGLVYALIAYGFQLTYATSKSLNFGQGEL
                     VMVSAFVSLTLLNTGLPYVVMVPLGILFGALLGVVVERVAVRLALQQKNEGWILLTII
                     VGLFLVSFAENVWGRDDRPFPTPIPADPVHVLGVDVTWLEISVAVGVLAIMALIEVLK
                     RRTLLGKAFAAVSADRDAAGLMGISATRTVTLSYALSGAVAAFAGILVSPITTVGPTM
                     AAALVLKAFSVAVVAGLESGFGVVLVGLILGALEGLASLYIGSGWRETPGLLLLILAL
                     AVRPTGLFGKAIIRKV"
     gene            9897..>11288
                     /locus_tag="VF516_35710"
     CDS             9897..>11288
                     /locus_tag="VF516_35710"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8113149.1"
                     /translation="MLKRLILVRGAEIALFVVLGALPLAVSNPFALLLLTLLAIYGVV
                     LIGLDVTVGYLGQVNLAQAAMLGLGAYLAGIAATRYGVGIPLAVVLSGVFCTVVGALL
                     ALPALRLEGPQFALATLSFTALCATALNELEWLTNGAQGLQIPRPLLLGHALDARDFF
                     WLCLAILALVWLAMRNLLSSQWGRAFEALRDSPIATDAMGVGTYRHKVAGFAFGSGLG
                     GAAGALYAFNLGYLQPQAFVYELTVILLLGVVLGGRKSLWGAVVGAVLVALLPNLLSS
                     HLLFRAFSGIAFALALVAGVRGWVRRTTDAFRALAPIAATLTLVAGSLVASNIEDWRK
                     AIFALLLFSVVVGLPEGLMGFASVGLARLFRVTPPELPPPAPLEHVLPARAPDGSRLL
                     ELAGLSRHFGGVRAVDGVDLAVKAGTIHGLIGPNGSGKSTLVNVVSGLYAPSGGQLRL
                     RGASLPRGKPVAAA"
BASE COUNT         1680 a         3970 c         3878 g         1760 t
ORIGIN      
        1 gcgagcgggc ccggcaggtg cgccgcggcc cagtcgacca ccggaccgac cgcgtgcacg
       61 accccgacca cggccgcgac caccagcacc agcgtgacgg ccgccggcgc cacgacccac
      121 ttccacagcg cggggtgcgc ccggagcacc gcgaggccgc gcccgacgtc gcgcgccccc
      181 gtgacgagct cgtgcatcgc gaccagcgta gcgtggaata ccaggcccgg caccctggct
      241 gccgcccctg ccaggccggc tgaaccggat ctcgcgcccg cgacggcggc gatctacatt
      301 cgcgccatgg ccgagcgctg ccggcgctgc gaagaggagc tgtcgaccaa ggcccggttc
      361 tgttcgaact gcggcttccc ggtctcggcg gccaacccgg gcgcggtcac cgaggacgcc
      421 accgccaccg agccgggcca gcaccggctg ccgccgcggc tgatcgcggc gggcacccgg
      481 atcaccgatc gctacatgat cgacggcgtg ctcggcgagg gcggcatggg cgtggtctac
      541 cgcgccaacg acctcgcgcg cgagcgcagc gtggcgatca aggcgctgca cgccagcctg
      601 atgggcgacg ccgagatccg gcgccggttc acccgcgagg cccagctgat gctgggctgg
      661 aaccaccgcc acgtcgcccg cgtccacgag atcatcgagc acccggacct gctcgcgttc
      721 gtcatggagt acgtcgaggg ccccacgctc gaggagcacg cgcagcgctg gaacggcaag
      781 ctgccgtacg acgagatccg ggtgatcttc agcggcgtgc tggacgcgat gcgagaggcc
      841 cacgccgccg gcatcgtcca ccgcgacctc aagccgcaca acgtgctgct ccgcatggag
      901 gacgacggcg tcatccccaa gatcgtcgac ttcgggatcg ccaagatcct cgagggcacg
      961 acctacacga tgaccggcgc gctgctcggc acctgccgct acatgtcccc ggagcaggcc
     1021 cagtcgccgc aggacgtcga tcaccgcacc gacatctact cgctcggcgt cacgctgtac
     1081 cgctgcgtca ccgggcggtg cccgttcgag ggcaacaacc acttcgcggt gatgatggcc
     1141 cacgtgtccc agccgcccga gccgccctcg gcgttccggc cgcacatgcc gccggcgctc
     1201 gagcagatca tcctgcgcgc gctcgccaag gaccgcgccg agcggccgca gtcgtgcgcc
     1261 gagttccgcg ccgcgctcga ggccgcgctg gtcgacgtca cgccgagccg ggtcgatcgc
     1321 gacaccgagt acccgcggat catcatcgag gacgacgaca acgagatgcg gctgatcccg
     1381 gccggctcgt tccagctcgg cgcgcaccgc cgcaccgtcg tgctcgaccg gttctacatc
     1441 gcgcgctacc cggtcaccaa ccgccagttc caggcgttcg tcgaggccac cggctacaag
     1501 ccgaccgacg ccgaggccca ccgcttcctg cagcacttcc gcaacggccg ctgcccgccc
     1561 gagctgctca accacccggt ggtgttcgta tcgtggaccg acgcgcgcgc gtactgccgg
     1621 tgggcggcgc gccggctgcc cagcgaggcc gagtgggaga aggcggcgcg tggccccgac
     1681 ggctacaagt acccgtgggg ccgcgacgag ccgaccgccg agcacgccaa cttcggccag
     1741 gcgcgcgcca agcactaccc gctcggcacc ggcggcacgg gcggcaccgc gccggtcgac
     1801 gcgttcccca ggagcgcgtc gccgtacggc atcgagggca tggccggcaa cgtgttcgag
     1861 tggtgcgagg acgtcgacga tccgggcttc tacctgcacg gccccgagcg caacccgcgc
     1921 aacacgatcc agcccggcaa cgcgccgtgc gtgatccgcg gcggctcgtg gcgctacgac
     1981 gtccgctcgc tcaggaccta cgcgcgcgcg agcttcgcgc cgacgttccg gctcgacacc
     2041 gtcgggttcc gcgtcgcgct gtgacgccga aaagatgata tcacggcgat ggaggcctcc
     2101 atgccacacg tcacccgccg gatcggactg tcgctcggcg ccgacatctg ctggccgatc
     2161 tgttacgagg agatcgtcaa gcgcctgaac ctcgcgatcc cctggcaggg cgacaccgtc
     2221 ggcatcgacg tcgagcgcgt cacgatcgag ccgttcgatc tgcgccagcc gtgccgctac
     2281 gacctggtga tcgaccggct gacccactgg tatcacacca gccgcgagtg gatcaaaaag
     2341 gccgtgatca tggacggcct ctacgtgttc aacaacccgt ggtcggtgca gtccaacgag
     2401 aagcagacca cctactgcgc gatgatgcac ctcgggatgc cgatccccga cacctggctg
     2461 atcccgccca agtcgtacga gccgctgccc gacctccagc ccacgctgcg cagctacgcc
     2521 aagctgttcg acctgggcgc ggtcggcaag cagctcggct acccgctgtt catgaagccg
     2581 tacgacggcg gcggctggaa gggcgtcagc aagatcgacg acgaggccgc gctgcgcgcg
     2641 cgctacgagg agagcggcaa gctggtgatg cacctgcaga aggcggtgca cccgttcgac
     2701 tcgttcgtgc gctgcatcgg cctggggccg cagacctacc tggtcaagta cgacccgggc
     2761 gcgccgctcc acgaccgcta cacgatggcc aagggcttcg tgcccgccga cgaggccgct
     2821 gtcctgcgcg acaccacgct gacgatcaac gcgttcttcg gctgggactt caactcgtgc
     2881 gaggcgctgc gcaaggacgg cacctggtac ccgatcgact tcgcgaaccc gtgccccgac
     2941 tcgcaggtca cctcgctgca ctaccacttc ccgtggctgg tcaaggccaa catccggtgg
     3001 tcggtgttca acgcggtgac ccggcggccg ttccgccgca cgctcgactg ggagccgttc
     3061 tacgcgatcg ccaggcgcga cctgccgtac cgcgagcggc tgcgcgagta cgcccgggtc
     3121 gccgagcagc ggttcgacgc cgccgcgttc gaggagttct gcgccaggca cctggcgcac
     3181 ctcgacgagg tcgcctggga gttcttcgcc acgccgaccg cgcgcgacgc ggtgaagaag
     3241 aaggtcgccg cgctgttccc ggcccacgag gtcgagctgt tcaccgagct gttctggcgc
     3301 cggatcggcg cctggcgcga cgaccacaag ccaggtgcca ccggagctgt gccatgacgc
     3361 tcgacgacgt caagacgatc ggccggtgga agtccgagcg gctcggccgc gaggtcacga
     3421 tggtccgctg gggcagcttc ggccagcctg tgctggtgtt cccgaccgcg ggcggcgacg
     3481 ccgaggagat cgagcgcatg cacctggtcg gcgcgctcgg cccgctgatc gaggccggcc
     3541 agatcaaggt ctactcgtgc gacagcgtcg ccggccgcgc cctgctgacc cgcgagggct
     3601 cgccgcagca ccagatgtgg ctgcaggacc agttccacca ccacgtccgc cacgaggtcg
     3661 tccccgcgat ccacgccgac tgcaagtccg agctcgacgt ctgggccgtc ggcgcgtcga
     3721 tcggcgcgtt ccattccgtc gcggtggtgt gccgcttccc ggatgtcttc gcgcgcgcgt
     3781 gcgcgatgtc ggggacctac gacctgcggc ggttctacga ggcgagcgag ttcaccgacg
     3841 cattctgggc atcgtcgccc ctgcacttcg tgccgaggct gtccggccac cacctcgacg
     3901 tgctgaagac ccggatgatc gtgctggcgt ccggcgaggg ccgcgccgag gacctcggcg
     3961 agtcgtggcg gatggccaac gcgctcggcg cccagggcat ccccaaccgg gtcgacccgt
     4021 ggggccccgg ctggcaccac gactggccga cctggcgcga gatgctgccg aagtacctcg
     4081 gcgaatgggt caaggaatag gcgcaagtcg ctagcgtttc cttgaccgct tgcgggtcgt
     4141 ggatcctttg cgtcggccag gcgctgggag ctcacgcagg cgggcagtca aggcagcgcg
     4201 acgtgggtga tcgggtggca acttccccag ccacgactcg agcaggtagt acgccgcgtt
     4261 cgggcgcttg tagcgtagcc acctgtccac caccacgagc attctatcga gtgtcatcga
     4321 attgccagga ccatgcacct tggcgaaaat gtcaagcgcg cgctgtgcgt gctcaagtgc
     4381 gagcttgtga tgaccgaggc atgtctctac cctgctcaca ttggcgaacg aaatcgcggt
     4441 atccggatgg tcatcgccca ggacgctacg ccggatgtcg agcgaacgct tcgcgagcac
     4501 gagtgcggtc tcgtgatctc cgagatcgtt atgggtgcca ccgaggttgt ccagcgccct
     4561 tgcggtctcg gggtgactct caccgaacac gcgggcgtgg acatgcagtg cctgctctct
     4621 cagctcgagc gccttcgcat gatcaccgag ggcggcatag acggctgcga cgttcatgag
     4681 cgacagcgcg gtgtccgggt gatcgtcgcc gagcatctcg cgccgggtcc tgagggctcc
     4741 ctcggcgtgc tccttcgcca gccgatgctc cccgagccac aggtaggtga gaccgaggtt
     4801 actgcgggac gtcgcggtcg tgggatgttc gtcaccgaac ctcttctgct cgatctcgaa
     4861 cgcttgtcga atgaacgtca gcgcggtctg gtgattgccg agatcgctgt atgcggcgcc
     4921 gacgttgccg aggagtatgg ccaggtcggg atgtcccgct cccaggagct cttcctcgag
     4981 ctgtagcgcg cgcgaatgga agtcgagcgc cttccggagg tcgccgagat ccgcatgcgt
     5041 cacaccgaca tcgttcaaga ccgacgcaat cgcacggtgc cgcggtccat gcacggcgag
     5101 cgacatggcg agtgcctgct cgctgagctt cagggcctgc ttgtagtcgc agagcccgcg
     5161 gtatgccatc gctacgtgac gcaacgatcg cgccgtgtcc gcatggccgt cgccatggcg
     5221 ctgccggtgc agctccagcg cgcgttgata gtacgcgagc gcgtcacgag ggttgccgag
     5281 cctgcgctcg agcgcgccca tgtcgctcgc gagcagcgcc tcgagatcgt cgccagccgg
     5341 agtctctcga tggtagcgct ccgcggcctt cacccactcg tgtgcttcgc gatagcgacc
     5401 gcggtggtag tgcggataac cctggagcca cgccagtcgc gcgcgccccg gccagcagcc
     5461 gtcgagcgcg tgccgctgcc acgcgcgcag atggtcgatc tcggcctcga acgccggtag
     5521 gtctgcaaac tccacgcgtc ggtgctcgaa ccaggcggcg agacggcgac tcacgtcctg
     5581 tgcccacgcc gtccggtgct cgatcgcgat ctcgtcgcgg cgtacttcct ggaccagccg
     5641 atggaggcgg tatcgctgag caccaccacc aaccggatcg tcctcgattc cgagcaggcg
     5701 cagcttcacg gcgagcgaca gcggcccgat gagcgccgtc tcgctgacgt cgagcagtgc
     5761 agcgagcaag gacgtgctca tggacgccga gccactcacg gtcaacacat cgagcacatc
     5821 gcggagccgc ggctcgtcgc cgagcatggc ctcggtcacg cgcagcgtcg cgtacaggtc
     5881 cttgtcatgg gtggtgaagc tctggaggaa tgagccagac agcgcctgct tgaggctgtc
     5941 atcgagcagg cgctgatagt cgcgccaggg catcggtgcg cgatgccgga ggtaagcgcc
     6001 cgcgatctcg agcgcgaggg gcagcccggc gaggcgcgcg gcgatcgctt gcgctgccgt
     6061 gcgctcgtcg ccgttcaccg gtgcacgccc ggcctcctgc accagcagct cgagcgattg
     6121 cgcggtgtcg aggacatcga gcgcgatcgg gtcgaagccg ggctgctcgg cgcggctggt
     6181 gaccaggatg tgcgcggccg cggcagggac cgggagatag cgctcgatcg ccgccgggtc
     6241 gttcacgttg tcgaacacga tgaggcagcc ggtgtgtgtg tggaggcggt gcagcgcgac
     6301 gtcgagcttg gcttggtgct cggagcgcgg gtcgacccac cgcgcctcgt cggagatctt
     6361 caccagctgc gcttcgatgt cgcgatccgc gtcgagccag aggacgccgc cggcgtacgt
     6421 cgcgccgtag cgatcggcgt actcgacggc aagctgggtc ttgccaagcc cgccgatgcc
     6481 ctggaacgac gcggtgtggc cgatcttctc tcatgtccgg ctcgtgagag aacgagtcga
     6541 tgtcacgaac cggcgctgag ctcgctcggc atcgcgtcga acacggtcgt catcgggcgg
     6601 gaggagccga tggcttcaag agcaatgtcc gagcttctcc gttcagccct gagatcgtct
     6661 tgacccagac gagcacctcg tcggcgctgg gaccgccctt tcgcctgccc gtcggcgacg
     6721 gatagcggta cgtggtcgca taggccgaga gcggctccag gacgcgaagc ttcgcgtgcc
     6781 acggatcgtc gtgtggcagc tcgtccacga ggcttcgagg tcgtgatcgg cggtgacgcg
     6841 caggccgcgg ctcagccgga cagccttgac gagcttctcg ccggcctgct gaagatgaaa
     6901 cgccgcgagt cggttcggtg gatccgcgac cagacgccgc gcggcgtcca gctcgagatc
     6961 gacgtcgtcc aggaacgcgg cgatcacgcg cggatcgggt gcgggaggct cagccgccat
     7021 agacgacgac gcctgtcgac gcgacgatct ccgcgagcgt gcccagcgag cgcttccatt
     7081 cgtcgaattc gctgcgcgtc atcgtgaaca cctcgacgcg ccggagccgc aggtctcgga
     7141 gccgctgcca caccgacgca aggtcgaggt cctgctcggg cgcatcgtca gccaggatcg
     7201 ccatgaagtc ccagtcgctg tcggcacgcg cctcaccgcg cgctcggctc ccgaacagcc
     7261 agacctgctc ggggtgcagc acctcgacga tgcgcgcgat catctccgcc ggaacgctcg
     7321 gcgaccgatc gagctgggca acagtctggg ccacatgccg atcataccgg attcgctggc
     7381 gttcgcaccc cggccgcgac cggcgcgctg ccgcatcagc gcgaggccca actgtccccg
     7441 cccgctgtcg agcatgcgct ccacgcccgc gagacggcgg tccgaggtcg gtcgacgaga
     7501 tctcccggct gcagggtctc cggcagcgac cgtccgcacc cacctgtcgc gcgcgcaagg
     7561 agatcgtcga gctcgcggcg cgatggtcga gcgagggcga cgggtgacag cccaccgcgt
     7621 cgaccggggg ggctcacgcc gccggagtgg ccggcgcggc gctggccggg tggtccgagg
     7681 ctgccggacg acctcggcgg cggcacacgt tgacagcgtt cgagcaccct cactaacatg
     7741 ccgccgtctg attgctttcc aaccatctca ccggaggtgc cccatgaagt ggctattggc
     7801 catggcgctc tgcgctgtca cgacacccgc gctggcacag tcgacgatca agatcgccat
     7861 caccggcccg ttcaccgggg ggtcggcccc gatgggcacc tccgcccgcg acggcgcgaa
     7921 gctggcgatc gccgagatca acgccgccgg cggcatccag gtcggcgcga agaagctcaa
     7981 gatcgaggcg atcgagcgcg acgacgaggc caagaacgag cgcggcgcgc tgatcggcca
     8041 ggagcttgcg gcgatgtcgg atctgtccgg cgtgatcggc accgtgaaca ccggcgtggt
     8101 gctcgccggc gacaagtacc tccaggacaa ggggatcacg cggatcatca cgccggcggc
     8161 cggctcggcc tcgatgacgc aatgggccaa gcccgacggt ccgaaggacc tgttcatgtt
     8221 ccggttcgcc gcccacgacg gcatccaggc ggcgatggtc gtcgaggagg cgatcgcgcg
     8281 gaagttcacc aaggtggcga tcttcgccga cgcgaccaac tacggggtgt ccggtcgcga
     8341 cgatctgctc gaccagatca agaagcaggg cggaaagctc caggtggtca ccatcgagaa
     8401 gtgggcgatc ggcgacaagg acatgactgc ccagctgctc aaggccaagg cgctcggcgc
     8461 gcaggcgctc ctgatctggg gcatcggccc cgagctcgcg gcgatcgcca acgggatggc
     8521 caagctcggt taccgggtgc cgatgatcgg cggctgggcg ctttcgatgt ccaacttcct
     8581 cgacaacgcc ggcgccaacg gcgacggcgc gatcatgccg cagaccttcg tcgaggagcc
     8641 ggtcacgccg cgggccaagg cgttcatcga ggcctaccac aaggcgtaca acaccacccg
     8701 catcccgtcg ccggtctcgg cggcgcaggg ctacgacgcg gtgctgctgt tcgccgccgc
     8761 cgtcaagcag gccggcacca ccgataccaa ggcgatccgc gatgcgctcg agaacctcaa
     8821 ggagccggtc gacggggtga tcgccgtctg gaccaagccg tactcgaagt ggaacccggc
     8881 cgacgtgacc acgcacgagg cgttccgccg cgagcaggtg gtgatgggac tggtgaaggg
     8941 cggccacgtg acgttcgcca acgagacgga ccgccagcgg ctcgccaagc ggggcgcgaa
     9001 gtagccgggc tcgatgccgc tttccgtcgt cgtccagatc gcggtcagcg gcgcgctgat
     9061 ggggctggtg tacgcgctga tcgcgtatgg cttccagctg acctacgcga cgagcaagag
     9121 cctcaacttc gggcagggcg agctcgtgat ggtgtcggcg ttcgtcagcc tgacgctgct
     9181 caacaccggc ctgccctacg tcgtgatggt gccgctcggg atcctgttcg gcgcgttgct
     9241 cggggtcgtc gtcgagcgcg tcgcggtgcg gctggcgctc cagcagaaga acgagggctg
     9301 gatcctgctg accatcatcg tcggcctgtt cctcgtgtcg ttcgccgaga acgtgtgggg
     9361 gcgcgacgat cgcccgttcc cgacgccgat tcccgccgac ccggtccacg tgctcggcgt
     9421 cgacgttacc tggctcgaga tctccgtcgc cgtgggcgtg ctcgcgatca tggcgctgat
     9481 cgaggtgctc aagcggcgga cgctgctcgg caaggcgttc gcggcggtct cggccgatcg
     9541 cgacgcggcc gggctgatgg ggatctcggc cacccgcacg gtgacgctgt cctacgccct
     9601 gtccggggcg gtcgcggcgt tcgccggcat cctggtctcg ccgatcacca cggtgggacc
     9661 gacgatggcc gcggcgctcg tgctcaaggc gttctcggtc gcggtcgtgg cgggcctcga
     9721 gtcggggttc ggcgtcgtgc tggtcggcct catcctgggc gcgctcgagg ggctcgccag
     9781 cctctacatc ggtagcggct ggcgcgagac gcccggcctc ctcctcctca tcctcgccct
     9841 ggccgtgcga cccaccgggt tgttcggcaa ggcgatcatc cggaaggtct aggcgcatgc
     9901 tcaagcggct catcctcgtg cgcggcgccg agatcgcgct gttcgtcgtg ctcggcgcgc
     9961 tgccgctcgc cgtgtccaac ccctttgcgc tcctcctgct caccctgctg gcgatctacg
    10021 gcgtcgtgct gatcggtctg gacgtcaccg tcggctatct tggccaggtc aacctcgccc
    10081 aggcggcgat gctgggcctc ggcgcttacc tcgccgggat cgccgcgacc cggtacggtg
    10141 tcggcatccc gctggccgtc gtgctgtccg gcgtgttctg caccgtggtc ggggcgctgc
    10201 tggcgctgcc ggcgctgcgg ctcgagggcc cgcagttcgc cctggcgacg ctcagcttca
    10261 ccgcgctgtg cgcgaccgcg ctgaacgagc tcgagtggct gaccaacggc gcgcagggcc
    10321 tccagatccc gcggccgctg ctgctcggcc acgcgctcga tgcgcgcgac ttcttctggc
    10381 tctgcctcgc gatcctggcg ctggtctggc tggcgatgcg caacctgctg tcgtcgcagt
    10441 gggggcgggc gttcgaggcg ctgcgcgaca gtccgatcgc caccgacgcg atgggcgtcg
    10501 gcacctaccg gcacaaggtg gcggggttcg ccttcggctc ggggctcggc ggcgccgccg
    10561 gggcgctcta cgcgttcaac ctcggctatc tccagcccca ggccttcgtc tacgagctga
    10621 ccgtcatcct gctgctgggc gtcgtgctcg gcggtcgcaa gagcctgtgg ggcgcggtgg
    10681 tcggcgcggt gctggtggcg ctgctcccca acctgctgtc cagtcatctg ctgtttcgcg
    10741 cgttctccgg gatcgccttc gcgctcgcgc tcgtcgcggg cgtgcgcgga tgggtccggc
    10801 gcacgaccga cgcgttccgg gcgctcgcgc cgatcgccgc gacgctcacg ctggtcgccg
    10861 gctcgctcgt ggcgagcaac atcgaggact ggcgcaaggc gatcttcgcg ctgctgctgt
    10921 tctcggtggt ggtcggcttg cccgaggggc tcatgggctt cgcgtccgtg gggctcgcgc
    10981 ggctgttccg cgtgacgcct cccgagctac cgccgcccgc accgctcgag cacgtgctgc
    11041 ccgcccgtgc gcccgacggg tcgcggctgc tcgagctcgc gggcctgtcg cgccatttcg
    11101 gtggtgtgcg cgccgtcgac ggcgtcgacc tcgcggtgaa ggcgggcacg atccacgggc
    11161 tgatcggtcc caacgggtcg ggcaagagca cgctggtcaa cgtcgtgtcc ggcctctacg
    11221 ctccgagcgg tggtcagctg cgcctgcgcg gcgcgtcgct gccccgcggc aagccggtgg
    11281 cggccgcc
//
LOCUS       DASVOR010001925         4372 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_487889,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001925 DASVOR010000000
VERSION     DASVOR010001925.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487889"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VF516_35715"
     CDS             complement(<1..234)
                     /locus_tag="VF516_35715"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8113150.1"
                     /translation="MAALRAKNGVEYPLAPQPHRWLVGSGANCDLVLDDPYVSATHCI
                     LERRIGGALVVRDRRSRNGTSIDSNPIETAELPI"
     gene            complement(1688..3091)
                     /locus_tag="VF516_35720"
     CDS             complement(1688..3091)
                     /locus_tag="VF516_35720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113151.1"
                     /translation="MQVISSVGRADERAQPGYLPRAYAAFFECNGPGSRGRLARHWLS
                     PRGVAHMKNLILGAAVAVLTAACGGGGKPQLLDASIDAQMVCNPIAQTGCMANQKCTW
                     IVDANANPTTMTAQIGHVDCVAVDATAIPDGMTCMPARAGVSGGADNCAKGDICISGV
                     CKQICDPQLASGAGSGACDTTHACLSYRGVFESTGIATAGACEAGCDPLTQQLATTKA
                     EACGSMDPTKPTGTCVPTSGEFKAFACAPALTGSLTLTDRMQPLGDSRGFFGNGCAPG
                     FLPFFFEDGSGSQKTLCSGMCAPVKMDSDIFKTATNNQPYGNTNALGKLPADPKAVAG
                     HATCQSTIKGALTDAEGTEDCRFIWLPLASSATPPAIVDTPYNNTLGMCFAYTKFSLT
                     NPNTKMTVPLKSCKDMSTTADPVLGTAEDQGCYPVAGFPGAAARVTPWLFKPNPHRLG
                     NFRAAYGPGVVARPVFD"
     gene            complement(3197..4195)
                     /gene="glpX"
                     /locus_tag="VF516_35725"
     CDS             complement(3197..4195)
                     /gene="glpX"
                     /locus_tag="VF516_35725"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEX8113152.1"
                     /translation="MQDRNLALEVVRITEAAALASARLMGRGDRKLADHVAVEAMRHA
                     FDHMDIRGTVVIGEGERDEAPMLFIGERVGAGWHDDSSSSPRVDIAVDPLEGTNLCAT
                     GAPDAISVIALADDGQFLNAPDTYMQKIAVGPEARGAIDLRESATWNLQRIARAKGKK
                     VEDCTAVILDRDRHKDLIAEVRQVGARIRLIGDGDVSGAVMTANPDSGIDVLFGTGGA
                     PEGVIAAAALKCVGGELQGRLRFRSDEERARAVRMGVKSEDTIYMTDDLARGDVMFAA
                     TGVTKGYLLDGVRFFGDSAKTESIVMRSKSGTVRVINATHHFNTKPKYSWATGLEP"
BASE COUNT          640 a         1445 c         1542 g          745 t
ORIGIN      
        1 gatcggcagt tcggctgttt cgatggggtt gctgtcgatc gacgtgccgt tgcgcgagcg
       61 ccggtcgcgc accaccagcg cgccgccgat ccgccgctcg aggatgcagt gggtcgccga
      121 tacgtacgga tcatcgagca ccagatcgca gttcgccccg gagccgacca gccagcggtg
      181 cggctgcggc gccagcggat actcgactcc gttcttggcg cgcagggccg ccacgacccc
      241 tctccgccgt gtgtcgggct cgagtgcctg ggtctcaccc tcgctctcat cacagaacgt
      301 catggccaat tcctccgtcc agtctttatc ggcacgccgg tgccgcggtt gcgtgaaact
      361 tgtatctcaa gcgccatgcc gcgctaaccg cgcgatctgc ggtggtgcgc tgtccggagt
      421 gtccgagcgg cggacggcgc cggacagcgc cggacggaca acaggcccca gcggtgccgg
      481 gcctgacggt tccggccgat ttcggcctcg acccgtcagc gatcgcgcat gacgggtttg
      541 atcgatttcg gccccgaccc gtccgcgatc cgatgacggg tttgatcgat ttcgacctag
      601 acccgtccgc gacgcggtga cgggtttgat cgttttcggc ctcgacccgt ccattcgacg
      661 gcaggccggg cagcgccggc acggctcgtc gtaggacgtc gggaatgacg cgggcgacga
      721 gtgcgcgacg catcgacatc tgccgtccgc gatgccgcca gggccgcgcg gccggcgccg
      781 gggcgtgcaa cccatgacgc gctggatgca tccgcccacg cgccagtccc cgggcgcgtc
      841 atgtcacctc gacccgctga tcgagggcgc tggccataga aggagtgctc gatgtcggcg
      901 ggaaggtctc tgtgatcggt cctggcgggg gaacggtgcg ctcaggtgcg gatgaaagtt
      961 cgagcgccgg aggtggcgga gctggattcg ccacgatggg tggcgcggga ggaagcatca
     1021 ccgcggatgg cggcggcggc cttggtggtg ggcaggccac cgatccatcg cttgtcaccg
     1081 tgctcattgg aggaacgtcc ccgaatttgt tggggttgcc actctcgaat ccgccgcgtc
     1141 cgggtggcgg tggaggcgcc gcaacgttga tcgcgtgtcg cggcgtggtg tcggtgagtg
     1201 gtacgatcga tgctgggggc ggcggaagcg gcgggggtgg agggctcacg gcaggagcca
     1261 agttctcggg caccgggggt ggttcggggg gcaacgtcgt tctccaaggc ctgtcgatcg
     1321 tcgtgaccgg ccaggtgttt gccaatggag gtggtggcgg cgctggggaa tttgtgccca
     1381 cgcaaggcac tgcacaggtt ggctttcctg gcagcaacgg gacacgatcg gcgactgacc
     1441 cggcctcggg aggaacacca ccgggtaccg ccggagcggg tggtactggc ggacgacaag
     1501 gcgcggcacc tggagttggc ttccatccgt cggatcccag tacaggcttg cccggcggtg
     1561 gcggtggcag cgtcggcttc ttccagacat atacaccgag cggcgtcact cctcagctga
     1621 ggccgctcgc tgcgtcccca acgttcagcc cgaacaagca ggtcaagacg cggtaatcgg
     1681 cactccgtca gtcgaaaacc ggtcttgcga cgactcccgg gccatacgca gcgcgaaagt
     1741 tacctaacct gtgcggattt ggcttgaaca gccatggagt cactcgtgcc gccgctccag
     1801 ggaaccctgc cacgggataa catccttggt cctcggctgt tccgagaacg ggatccgcgg
     1861 ttgtgctcat atctttgcag ctcttcaagg gaaccgtcat cttggtattg ggattcgtca
     1921 aggagaactt cgtataggca aaacacatcc cgagcgtgtt gttgtacggc gtatccacga
     1981 tggccggtgg agttgctgaa ctcgcaagcg gcaaccagat gaagcgacaa tcctcggtgc
     2041 cctcggcatc ggtgagcgcg cccttgatcg tggactggca cgtcgcatgt cccgccaccg
     2101 ccttcgggtc cgccggtagc ttgccgagcg cgttggtatt accatagggc tggttgttgg
     2161 tagcggtctt gaagatgtcg ctgtccatct tgaccggcgc gcacatgccc gagcacagcg
     2221 tcttctggga gcccgagcca tcctcgaaga agaacggcag gaagccgggc gcgcagccgt
     2281 tgccgaagaa gccgcgcgag tcgcccagcg gctgcatccg atcggtgagt gtgaggcttc
     2341 ccgtcagcgc cggggcgcag gcgaacgcct tgaactcgcc gctggtgggg acgcaggtgc
     2401 cggtcggctt ggtcgggtcc atcgagccgc acgcctcggc cttggtggtc gccagctgct
     2461 gggtgagcgg atcgcagccg gcctcgcagg cgccggcggt ggcgatcccg gtcgactcga
     2521 acacgccgcg gtagctcagg cacgcgtggg tcgtgtcgca ggcgcccgag ccggcgccgg
     2581 aggcgagctg cggatcgcag atctgcttgc acacgccgga gatgcagatg tcgcccttgg
     2641 cgcagttgtc ggcgccgccg ctgaccccgg ccctggcagg catgcaggtc atgccgtcgg
     2701 ggatcgcggt cgcgtcgacc gccacgcagt ccacgtggcc gatctgggcc gtcatggtgg
     2761 tgggattggc gttggcgtcg acgatccagg tgcacttctg gttggccatg cacccggtct
     2821 gcgcgatggg gttgcagacc atctgcgcgt cgatgctcgc gtcgaggagc tgcggcttgc
     2881 cgcccccgcc gcaggcggcg gtgagcacag cgacggcagc tcccaggatc aggttcttca
     2941 tgtgcgcgac tcctctaggg gaaagccaat gcctggccaa tcttccccgg gaacccggtc
     3001 cgttacactc aaaaaacgca gcataggctc tcggcaggta cccaggctgc gctcgctcgt
     3061 cggcccggcc gacgctggag atgacctgca tcacgctctg cggcagcgcg gcagcgaccg
     3121 gcgccgccct gggacgacgc cccagcgcag acggtggcta caggtcggag gcgtttgtcg
     3181 cacccgtggg ccggcgtcag ggctccaggc cggtcgccca cgagtacttg ggcttggtat
     3241 tgaagtggtg ggtcgcgttg atcacccgca cggtgccgga cttggagcgc atgacgatcg
     3301 actcggtctt ggcgctgtcg ccgaagaacc gcacgccgtc gagcaggtag cccttggtga
     3361 cgccggtggc ggcgaacatg acgtcgccgc gcgcgaggtc gtcggtcatg tagatcgtgt
     3421 cctcgctctt gacccccatc cgcacggcgc gcgcccgctc ctcgtcgctg cggaaccgca
     3481 ggcggccctg gagctcgccg ccgacgcact tgagcgccgc cgcggcgatc acgccctcgg
     3541 gcgcgccgcc ggtgccgaac agcacgtcga tgccggagtc cgggttcgcg gtcatcaccg
     3601 cgcccgagac gtcgccgtcg ccgatcaggc ggatccgggc gccgacctgg cggacctcgg
     3661 cgatcaggtc cttgtggcga tcgcggtcga ggatcaccgc cgtgcagtcc tcgaccttct
     3721 tgcccttggc ccgcgcgatc cgctggaggt tccaggtcgc cgactcgcgc agatcgatcg
     3781 cgccgcgcgc ctcggggccg actgcgatct tctgcatgta ggtgtccgga gcattgagga
     3841 actggccgtc gtcggcgagc gcgatcacgc tgatcgcgtc gggcgctccg gtcgcgcaca
     3901 ggttggtgcc ctcgagcgga tcgaccgcga tgtcgacccg cggcgagctc gagctgtcgt
     3961 cgtgccagcc cgcgccgacc cgctcgccga tgaacagcat cggcgcctcg tcgcgctcgc
     4021 cctcgccgat cacgacggtg ccgcggatgt ccatgtggtc gaacgcgtgc cgcatcgcct
     4081 cgaccgcgac gtgatcggcg agcttgcgat cgccgcggcc catcaggcgg gcggacgcca
     4141 gcgccgccgc ttcggtgatg cggaccactt cgagcgcgag gttgcggtct tgcatgacga
     4201 aaggtgtacc cctgcccgcg ccggccgatc aagtgcgtcc cgcgactcgc gtcgatctca
     4261 ccgcggcccg gcaagccgcg cggcgccggg cggatccgcg cgccggtcag ccgacccgcg
     4321 ggatagcgtc gcgcagcgcg ggcggcagcg cccgcagccg gccgtccttg cc
//
LOCUS       DASVOR010001926         3608 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_282162,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001926 DASVOR010000000
VERSION     DASVOR010001926.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3608
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282162"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..692)
                     /locus_tag="VF516_35730"
     CDS             complement(<1..692)
                     /locus_tag="VF516_35730"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8113153.1"
                     /translation="MESQATRCSAARKIDTLVFNLNFRCDKRCSMCCVGATPSARAAD
                     LPLETIAAVARQIAEAGIEVDAIGLSGGEPFLYGGADEGGHTLADVIRVLCDAAPRAI
                     HVQTSGVGPMVRDFARQLDDVFAAAAARGVAIEIESSYTAYQIPGIDERMAATIEAVL
                     AHQDRFMIYLTSDPDNARQTLLGFRGLLESMGFTKQPPPARDLDQADGEARAPGGDGD
                     RHLFRFARGGKQV"
     gene            953..2212
                     /locus_tag="VF516_35735"
     CDS             953..2212
                     /locus_tag="VF516_35735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NHLP bacteriocin system secretion protein"
                     /protein_id="HEX8113154.1"
                     /translation="MKDLFRKSALEHLASPDQLHAALKVTDRKGWLALLACFAILATA
                     LVWSAYGRLPTRVQGRGILVSKGGLNTVNAPTSGQVAMVNATVGSHLARGDVVARLLQ
                     QDLDAELRNAQATLATLEDEHRRLTAFGIDEARLHAQAAAQQRATALATQAMARERQT
                     LLAERLAAQQRLVADGLITDAVLQATRDNLNLATGEIKRAQTTLAEIDLAEGQNEQRR
                     RREFDERVQRIDEARRKADALDAKVKTTSLVVSQVAGQVLEVRTAPGQFVTAGTQLVM
                     IELDDEGHAGMEALVYLPGTDGKRLQPGMVAEISPSTVKREEHGAMRGVVTAVSSFPA
                     TQQAMLTALGNPDLVAGLFKIIDSPIQVKVDLVGDRSTTSGYPWTSRRGPATTIQPGT
                     LCTAAITVREQAPITLVLPRLREWLGL"
     gene            2272..>3608
                     /locus_tag="VF516_35740"
     CDS             2272..>3608
                     /locus_tag="VF516_35740"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX8113155.1"
                     /translation="MEAVECGAAALGIVLGHFGRYVPLEVLRIECGVSRDGSKTGNVL
                     RVARKFGLEAKAFKMEVEGIRTAEPPLIVFWNFNHFLVVEGFGRNKVYLNDPSTGPRT
                     VSFEEFDRSFTGVVLSFRPGPDFTPGGERERLVASLGGRLRGSRIAFTFVVATGLALI
                     GPGLLVPTLGRVLVDEVLVGGAVLWAPAILLSLALTAVLRGALVALQKRYLGRLETRL
                     ALSMASRFMWHVLRLPVEFFTQRHAGDVTMRVLLNDQVARLLSGELAANVLNLFTIAF
                     YAAILILYDRVLGLFCVAVAVINMILLYRVARRRADQNLALQLDIARLHSTGIGGLQT
                     IETIKATGSEAEFFARWTGFQAKVVNGQQRFGEATVKLAAAPPLLSSLATIATLGLGA
                     LRVMDGQLSMGMLVAFQSLMASFLLPVNELVRLASTLQEAHSGLRRLDDALRYR"
BASE COUNT          511 a         1250 c         1321 g          526 t
ORIGIN      
        1 acctgcttgc ccccgcgcgc aaagcggaac aggtgccgat cgccgtcgcc tcccggcgcg
       61 cgcgcctcgc cgtcggcctg gtcaaggtcg cgggcgggtg gcggctgctt ggtgaagccc
      121 atcgactcga gcagcccgcg aaagccgagc agcgtctgcc gcgcgttgtc gggatccgag
      181 gtgaggtaga tcatgaaccg gtcctggtgc gccagcaccg cctcgatggt cgcggccatg
      241 cgctcgtcga tgccggggat ctggtaggcg gtgtagctgc tctcgatctc gatcgccacg
      301 ccgcgcgccg ccgcagccgc gaacacgtcg tcgagctgcc gggcgaagtc gcggaccatc
      361 ggcccgacgc ccgaggtctg cacgtggatc gcccgcggcg cggcgtcgca cagcacgcgg
      421 atcacgtcgg cgagggtgtg gccgccctcg tcggcgccgc cgtacaggaa cggctccccg
      481 cccgacaacc cgatggcatc gacctcgatc ccagcctcgg cgatctggcg cgcgaccgcc
      541 gcgatcgtct cgagcggcag gtccgcggcc cgcgcgctcg gcgtggcgcc cacgcagcac
      601 atcgagcagc gcttgtcgca ccggaagttg agattgaaga cgagcgtatc gatcttcctt
      661 gccgcactgc accgggttgc ttgcgactcc atggtgatct cccagccatt gccgccgagc
      721 cggcagcacc ctctgcctgc gaatcgcgcg ccgtccactg ccgttcgcga accggcgcag
      781 gcgcgaatga cctgcagaca cggcccgcgc ggtccgcagc ttttgcgcgt tcgaagtcga
      841 ggctgccccg ggccgctacg actgcgcatc gtcgcgagcg agtcaggagg cccctggctt
      901 gcatccctcg ccagcgcgag gccagcgctc gaccctcaca aaggacatcg acgtgaagga
      961 tctctttcgc aagtccgcgc tggagcacct cgcgtcgccc gaccagctcc acgctgcgtt
     1021 gaaggtcacc gatcgcaagg gctggctcgc gctcctggcg tgcttcgcga tcctggcgac
     1081 cgcgctcgtg tggtcggcct acgggcggct ccccacgcgc gtgcagggcc gcggcatcct
     1141 ggtcagcaag ggcgggctca acaccgtcaa cgcgccgacc agcgggcagg tcgcgatggt
     1201 gaacgcgacg gtgggctccc acctggcccg gggcgacgtg gtcgcgcgcc tgctccagca
     1261 ggatctcgac gccgagctgc gcaacgcgca ggcgacgctc gcgacgctgg aggacgagca
     1321 ccggcggttg accgcgttcg gcatcgacga ggcgcgcctg catgcgcagg cggcggcgca
     1381 gcagcgcgcg accgcgctgg ccacgcaggc gatggcgcgc gagcgccaga cgctgctcgc
     1441 cgagcgcctg gcggcgcagc agcggctcgt cgccgatggc ctgatcacgg acgcggtgct
     1501 gcaggcgacg cgcgacaacc tcaacctggc caccggcgag atcaagcggg cccagaccac
     1561 gctcgccgag atcgacctgg ccgaggggca gaacgagcag cgccgccgcc gcgagttcga
     1621 cgagcgcgtg cagcgcatcg acgaggcgcg gcgcaaggcc gacgcgctcg acgccaaggt
     1681 caagaccacg tcgctggtgg tgagccaggt ggcgggccag gtgctcgagg tgcgcaccgc
     1741 gcccgggcag ttcgtgaccg cgggcacgca gctggtgatg atcgagctcg acgacgaggg
     1801 ccacgcgggc atggaggcgc tggtctacct gcccggcacc gacggcaagc gcctgcagcc
     1861 gggcatggtg gccgagatct cgcccagcac ggtcaagcgc gaggagcacg gcgcgatgcg
     1921 cggcgtggtg accgcggtgt cgagcttccc cgcgacccag caggcgatgc tgaccgcgct
     1981 cggcaatccg gatctggtcg ccgggttatt caagatcatc gactcgccga tccaggtcaa
     2041 ggtggacctc gtgggcgacc ggtcgaccac gagcggctac ccgtggacct cgcgccgggg
     2101 accggcgacc accatccagc caggcacgct gtgcacggcg gcgatcacgg tgcgcgagca
     2161 ggcaccgatc accctggtgc tgccgcggct ccgcgagtgg ctcgggctat gaccgcggcg
     2221 gcgacaaccg cgaagcgctg ggcgcgcgtg cgcacgccga cggtgttgca gatggaggcg
     2281 gtggagtgcg gcgcggcggc gctggggatc gtcctcggcc acttcggccg ctacgtgccg
     2341 ctggaggtgc tgcgcatcga gtgcggcgtg tcgcgcgacg gcagcaagac cggcaacgtc
     2401 ctgcgggtag cgcgcaagtt cgggctggag gccaaggcgt tcaagatgga ggtcgagggc
     2461 atccggaccg ccgagccgcc gctgatcgtg ttctggaact tcaaccactt cctggtggtg
     2521 gaggggttcg gacgcaacaa ggtctacctc aacgatccga gcaccggccc gcgcaccgtc
     2581 agcttcgagg agttcgatcg cagcttcacc ggcgtcgtgc tcagctttcg cccggggccg
     2641 gacttcacgc cgggcgggga gcgcgagcgc ctcgtcgcgt cgctcggcgg gcgcctgcgc
     2701 ggatcgcgga tcgcgttcac gttcgtggtg gcgaccggcc tcgcgctgat cgggccgggg
     2761 ctgctcgtgc ccacgctcgg gcgagtgctc gtcgacgagg tcctcgtggg cggggcggtg
     2821 ctgtgggcgc ccgcgatcct cctgtcgctc gcgctcaccg cggtgctgcg cggcgcgctg
     2881 gtggcgctgc agaagcgcta cctcgggcgg ctggagaccc ggctggcgct gtcgatggcc
     2941 agccggttca tgtggcacgt gctgcggctg ccggtggagt tcttcaccca gcgccacgcg
     3001 ggcgatgtga ccatgcgggt cttgctcaac gaccaggtcg cgcgcctgct ctcgggcgag
     3061 ctggcggcca acgtcctcaa cctgttcacg atcgccttct atgcggcgat cttgatcctc
     3121 tacgatcgcg tgctcgggct gttctgcgtg gcggtggcgg tgatcaacat gatcctgctc
     3181 tatcgcgtgg cgcggcgtcg cgccgaccag aacctggcgc tccagctcga catcgcgcgc
     3241 ctgcactcga ccggcatcgg cgggctgcag acgatcgaga cgatcaaggc gacgggcagc
     3301 gaggccgagt tcttcgcgcg ctggaccggg ttccaggcca aggtggtcaa cgggcagcag
     3361 cggttcggcg aggcgaccgt gaagctggcg gcggcgccgc cgctgctgtc gtcgctggcg
     3421 accatcgcga cgctggggct cggcgcgctg cgggtgatgg acggccagct cagcatgggg
     3481 atgctggtgg cgttccagag cctgatggcg agcttcctgc tgccggtcaa cgagctggtc
     3541 cggctggcga gcacgctgca ggaggcgcac agcgggctgc ggcggctcga cgacgccctg
     3601 cgctatcg
//
LOCUS       DASVOR010001927         8145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_137068,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001927 DASVOR010000000
VERSION     DASVOR010001927.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8145
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137068"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VF516_35745"
     CDS             complement(<1..323)
                     /locus_tag="VF516_35745"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEX8113156.1"
                     /translation="MKDTLKVGLASTKRITVDEGRCIGFMGKEGMVYATPRMVSDVEY
                     ACRDLLLAHLDEGEDSVGAHVSIDHLAATPLGLDVTIEVRIADIEKRRVAFEFSVRDP
                     VEECGR"
     gene            complement(414..1940)
                     /locus_tag="VF516_35750"
     CDS             complement(414..1940)
                     /locus_tag="VF516_35750"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8113157.1"
                     /translation="MVRRSGDSDELFRLLVASVRDYAIFMLDPTGHVATWNIGAERIK
                     RYRAGDIIGRHFSVFYPDEDVRAGKCEMELEVAARDGRFEDEGWRIRSDGTRFWANVV
                     ITAIRDSAGTLVGFAKVTRDLTERKRNEDERAARLAAEQANRAKDEFLAVLGHELRNP
                     LAPIVTALQLIKLRGDTPSPREVQVLERQVQQLMRLVDDLLDVSRIARGKLELKLQGV
                     DLRGPISRALEIASPLVEHKRHHFEVKVPPYALLVHGDEARLTQVFANLLTNAAKYTD
                     PGGHLFLRVWQAGAEIVIEVRDDGIGIDKDLMPRIFDPFVQGAQNGERAAGGLGLGLG
                     LVRSLVELHGGHVEVHSQGARLGSTFTVHLPAVSQPPSRETGQQIAAAFSTEAGKHRI
                     LVVDDNEDARGLLADILRQLGHDVRAAGDGPQALAMVREFTPDVAVLDIGLPDMDGYE
                     LAAQLRRALPATPVRLIALSGYGQASDRARSKAAGFDGHLVKPVEVRRLLDAIATAGR
                     "
     gene            complement(2293..2865)
                     /locus_tag="VF516_35755"
     CDS             complement(2293..2865)
                     /locus_tag="VF516_35755"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RICIN domain-containing protein"
                     /protein_id="HEX8113158.1"
                     /translation="MPRASVFGYGLCLALGAVTTGCLADGGLDENEATVSSEISLDPT
                     ASYTLVGVQSNKCVGPIGGSSALGVQLEIENCTGTANQRWIPTPVAGGFFRWKNELNG
                     LCIDVSGGSISAGAAVIQWTCGTQTNQQWTVTDVTGGSERMTARHSGMVLDVTGQGTA
                     AGTKVEQWFSNGGSNQHFVMDEALPALVGP"
     gene            complement(3391..4308)
                     /gene="bla"
                     /locus_tag="VF516_35760"
     CDS             complement(3391..4308)
                     /gene="bla"
                     /locus_tag="VF516_35760"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HEX8113159.1"
                     /translation="MRLHRSLTAFLALTLGPPLAATARAQSPAPAQAPAAPSLANADW
                     TEPFPPFRIAGNLYYVGSKGLASYLITTPKGHILINSDLEASVPLLQDSIAKLGFKIT
                     DVKVLLISHAHWDHDAGSAAIKKLTGASYMVMDADVPVVESGGKTDFQYNTKPEWLYP
                     PTKVDRVLHDGDEVKLGGTTLVAHLTPGHTRGCTTWTMKVSDGGKQLDAVIIGSPNVN
                     PGYKLVGNKLYPKIADDYRRMFKVLGGLHCDLFLGAHGSYFGMTDKYPRLQPEAPNPF
                     IDPDGYKKFVAQKEQEFRQELDKQTAAAK"
     gene            complement(4395..6608)
                     /locus_tag="VF516_35765"
     CDS             complement(4395..6608)
                     /locus_tag="VF516_35765"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX8113160.1"
                     /translation="MTRPLIGTGIDRVDGRKKVTGGATYAAEVQVANVTYAVIVGSTI
                     GRGKIKAIDTAAAQAAPGVLAVVTHDNAPKFPGVSGNHEANDRVVQGLQDSVVLYHDQ
                     PIAVVVADTLERAQHAAALVTAEYDAAPPVGDVEADLGSAYIFQTPRGPAEIIRGDFS
                     AGMAAAKTRIDATYRTPVETHNPMEPHATIAVWQGEDHLTLYDSTQAIFNVRRRLSTM
                     LGLPAENVRVINHFVGGGFGCKGAPWSHVLLAAIAAKATSRAVKLAITRPQMQSLVGH
                     RPKTVQKLELGLDAAGRLTAIRHDCLSETSRYDVFVESCTAVTRMLYACPNVATVQRL
                     VRLDVPTPTFTRAPGESVGTFALESALDELAHAAGIDPLELRRKNHADHDPETGKPWS
                     SKSLLECYQRAAEAIGWSKRPAQIKTLREGRWQIGYGVATTTRGSRQLPASARATMRP
                     DGRALVQAGTQDIGTGTYTVMTQIAGDALGLPIGDIRFELGDTALPEAPPSVGSFTAA
                     STGPAVHNACVALRKKLIEQAVGDPASPLHGLAPDAIDAEDGALIAKADRKRRDPYAA
                     IVARSKQPEISVELHTEAHKDRDQYSVGGFGAVFAKVRVDEDLGEIRVDRLVGAFAAG
                     RILNPKTASSQLLGGMVWGIGQALHEHSVRDPRTARMVTRDLADYHVPVHADVPQLEV
                     ILVHEEDPHVNEIGVKGLGELSTPGVAAAIANAVYHATGIRIRDLPITPDKLIQG"
     gene            complement(6605..7585)
                     /locus_tag="VF516_35770"
     CDS             complement(6605..7585)
                     /locus_tag="VF516_35770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX8113161.1"
                     /translation="MKPFTYVRPADARAAIAAASDGSASFLAGGTTLVDLMRLEVMTP
                     SAVVDLSPLPLTQIERAGDGLRIGAMARNSAVATHPLVVERYPLLAQALLAGASQQVR
                     NMATVGGNLLQRTRCPYFRDLAVACNKRTPGSGCSAIDGFTRSHAILGTSESCIATHP
                     SDMCVALAALDAVVHTRGAGRERAIPFGEFHTLPGDHPEVENALEPGELVTHVELPAS
                     PFAAHAHYVKVRDRAAFAFALASAAVGLDVADGKIRAARIALGGVATRPWRAVEAEHA
                     LVGQAPNRTSFERAAAAAIVDPKPRRDNAFKIALAKRTIVRALELAWGAA"
     gene            complement(7587..>8145)
                     /locus_tag="VF516_35775"
     CDS             complement(7587..>8145)
                     /locus_tag="VF516_35775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8113162.1"
                     /translation="VAGFSVVLFHGCRTPGADRPPAPAPVAVKAGSEVDVVLRINGQD
                     RRLRIDPRTSLLDALREHLALTGARKGCDHGQCGACTVLRDGHAVNACLTLAVAAEGA
                     EITTIEGLARGDQLHPVQSQFITHDAFQCGYCTSGQILSAVALLREGRAKTDDEVRQQ
                     MSGNICRCGAYPNIVAAIQAARKEV"
BASE COUNT         1100 a         2725 c         2999 g         1321 t
ORIGIN      
        1 cggccgcatt cctcgaccgg gtcgcggacc gagaactcga acgccaccct gcgcttctcg
       61 atgtcggcga tccggacctc gatcgtgacg tcgagcccga gcggcgtggc ggcgaggtgg
      121 tcgatcgaga cgtgggcgcc gaccgagtcc tcgccctcgt cgaggtgcgc gagcaggagg
      181 tcgcggcacg cgtactcgac gtcgctcacc atccgcggcg tcgcgtagac catgccctcc
      241 ttgcccatga agccgatgca gcggccttcg tccaccgtga tgcgcttggt cgaggcgagc
      301 ccgaccttga gggtgtcttt catggccgca ttgtcctaca acgcgcgcgg gacatgctcg
      361 cagcaggcgg cgaacctggc gagtgcgagc cagctcgcct cgcgggacgc gggtcagcgg
      421 cccgcggtcg cgatggcgtc gagcaggcgg cggacctcga cgggcttgac caggtggccg
      481 tcgaagcccg cggccttgct gcgcgcgcgg tccgaggcct ggccgtagcc gctgagcgcg
      541 atcaggcgca cgggggtggc cggcagcgcg cggcgaagct gcgcggcgag ctcgtagcca
      601 tccatgtcgg gcagcccgat gtcgaggacc gcgacgtccg gcgtgaactc gcggaccatg
      661 gccagcgcct gcggtccgtc gccggcggcc cggacgtcgt ggccgagctg cctcaggatg
      721 tcggcgagca gcccgcgcgc gtcctcgttg tcgtcgacca cgaggatccg gtgcttgccg
      781 gcctcggtgg agaatgcggc ggcgatctgc tggccggtct cgcgcgacgg cggctgggac
      841 accgccggca agtggacggt gaacgtgctg cccagccggg cgccctggct gtgcacctcg
      901 acgtggccgc cgtgcagctc gacgagcgag cggacgagcc cgagaccgag gccgaggccg
      961 ccggcggcgc gctcgccgtt ctgcgcgccc tggacgaacg ggtcgaagat ccggggcatg
     1021 aggtccttgt cgatgccgat gccgtcgtcg cggacctcga tcacgatctc ggcaccggcc
     1081 tgccagaccc gcaggaagag gtggccgccc ggatcggtgt acttggcggc gttggtcagc
     1141 aggttggcga acacctgggt caggcgagcc tcgtcgccgt ggaccagcag cgcgtagggc
     1201 gggaccttga cctcgaagtg gtggcgcttg tgctcgacga gcgggctcgc gatctcgagc
     1261 gcgcggctga tcggaccccg gaggtcgacc ccctgcagct tgagctcgag cttgccgcgt
     1321 gcgatgcgcg agacatcgag caggtcgtcg accaggcgca tcagctgctg gacctggcgc
     1381 tcgaggacct ggacctcgcg aggcgacggg gtgtcgccgc gcagcttgat cagctgcagc
     1441 gcggtgacga tcggggccag cgggttgcgc agctcgtggc cgagcaccgc gaggaactcg
     1501 tccttggccc ggttggcctg ctcggcggcg agccgggcgg cgcgctcgtc ctcgttgcgc
     1561 ttgcgctcgg tgagatcgcg ggtgaccttg gcgaacccga cgagcgtgcc ggcggagtcg
     1621 cggatcgcgg tgatcacgac gttggcccag aaccgcgtgc cgtcgctgcg gatccgccag
     1681 ccctcgtcct cgaaccggcc gtcgcgcgcg gcgacctcga gctccatctc gcacttgccg
     1741 gcgcgcacgt cctcgtcggg atagaacacc gagaagtggc ggccgatgat gtcgccggcg
     1801 cggtagcgct tgatccgctc ggcgccgatg ttccaggtcg cgacgtggcc ggtcggatcg
     1861 agcatgaaga tcgcgtagtc gcggacgctc gcgaccagca ggcggaacag ctcgtcggag
     1921 tcaccactcc tgcgcaccat cgccgccagc cggtcctgcg gatcccgctc gcggtgtgcc
     1981 tgcggcgctc gctctcggtg aaccgcgtcg agctgcgctc cggcctctgt gagctcctgg
     2041 tgcaacgttt gccagcagga tagcgatccg gagcgcgttt gcatcgctcg tctttacgaa
     2101 atctttagca accggccgtg cgccaccgcc cacgccgcgc acacgttccg cgcggcctcc
     2161 gagcacctcg gtttttctga gccaccgagc agatccccgc ggtttcggtg gcgcgcgacc
     2221 ggcgcggcct cgcatgggcc gtgcgctatt gccacatcgg gccgccgcgc acgggtgcga
     2281 gagacggcgc gatcaggggc cgacgagcgc gggtagcgcc tcgtccatga cgaagtgctg
     2341 gttgctgccg ccgttcgaga accactgctc gaccttcgtc ccggcggcgg tgccctggcc
     2401 ggtcacgtcg aggaccatgc cgctgtggcg cgccgtcatg cgctcggagc cgccggtgac
     2461 gtcggtgacc gtccactgct ggttggtctg ggtgccgcat gtccactgga tgacggcggc
     2521 accggcggag atcgagccgc cggacacgtc gatgcagagg ccgttcagct cgttcttcca
     2581 ccggaagaat ccaccggcca ccggcgtggg gatccagcgc tgattggccg ttccggtgca
     2641 gttctcgatc tcgagctgca cgcccaaggc cgagctgcca ccgatcgggc cgacgcactt
     2701 gttgctctgc acgccgacca gggtgtacga cgccgtggga tcgaggctga tctcggagct
     2761 gacggtcgcc tcgttctcgt cgagaccacc gtcggccagg cacccggtcg tgacggcgcc
     2821 gagggcaaga cacagcccat aaccaaacac ggacgctcgc ggcatagata cgatcctcat
     2881 ggcaaaccct ccatgaaacc gcagtgcaaa cccgactgtc gggccgcacg ctgttcaatg
     2941 gccaccccta tatcattttt ctctcgcacg gaatcccatt atttcggatt tcgttccgtg
     3001 gtgtttcaga gtgcgaactg cacgacggtt gctaagtgag acaaagcttg cgctgtgacc
     3061 ggccgagcaa gagcctcccc tcaccggtga tgtcgcgcgg cgacttcgcc gaggttctcg
     3121 tcggagatcg gcgctggctg ccgttttctg tggttcttcg tcacgtgggc attcgtacga
     3181 ataccgcgca gatcaatagg tgtgaatgaa tctttgtcac gtgattcatc gcacgaatgc
     3241 cgtacagggg aatccgcacg atggtgcgat cgtccgaccg gttgtcatgg gcgctcacgc
     3301 cgatctgcac atcacattgc gttgtgagcg ccagttgacg cgtattgcgg cgccgtgtgc
     3361 tctgccgcgc atcgccggcg aggcgccgca ttacttcgcg gcggcggtct gcttgtcgag
     3421 ctcctggcgg aactcctgct ccttctgcgc gacgaacttc ttgtacccgt cgggatcgat
     3481 gaacgggttg ggcgcctcgg gctggagcct ggggtacttg tcggtcatgc cgaagtagct
     3541 gccgtgggcg ccgaggaaca ggtcgcagtg cagcccgccg agcaccttga acatgcggcg
     3601 gtagtcgtcg gcgatcttgg ggtagagctt gttgccgacc agcttgtagc cggggttgac
     3661 gttggggctg ccgatgatga ccgcgtcgag ctgcttgccg ccatcgctga ccttcatcgt
     3721 ccacgtcgtg cagccccggg tgtggccggg cgtcaggtgg gcgaccagcg tggtgccgcc
     3781 gagcttgacc tcgtcgccgt cgtgcagcac gcgatcgacc ttggtcggcg ggtacagcca
     3841 ctcgggcttg gtgttgtact ggaagtcggt cttgccgccc gactcgacga ccggcacgtc
     3901 ggcgtccatc accatgtagc tcgcgccggt cagcttcttg atcgccgcgc tgccggcgtc
     3961 gtgatcccag tgggcatggc tgatcagcag caccttgacg tcggtgatct tgaacccgag
     4021 cttggcgatg ctgtcctgga gcagcggcac gctcgcctcg aggtcgctgt tgatcaggat
     4081 atggcccttg ggcgtggtga tgagatagct ggcgagtcct ttactcccca cgtaatagag
     4141 atttcccgcg atacgaaatg gcgggaatgg ctcggtccag tcggcgttgg cgaggctggg
     4201 cgcagccgga gcctgggccg gggctggaga ttgagcgcgc gccgtcgcgg ccagcggcgg
     4261 gcccagggtg agcgcgagga acgcggtgag tgatcggtgc aggcgcattc ggtacctccg
     4321 tggcgcgcat catcgcaccg gcgccgtgaa tccgaacccg cccggagcac tctggagaga
     4381 ggatccgatg gtccctagcc ctggatcagc ttgtcgggcg tgatcggcag gtcgcggatc
     4441 cggatgccgg tcgcgtggta gaccgcgttg gcgatcgcgg cggcgacgcc gggcgtcgac
     4501 agctcgccca ggcccttgac cccgatctcg ttgacgtggg ggtcctcctc gtggacgagg
     4561 atgacctcga gctgcggcac gtcggcgtgg accgggacgt ggtaatcggc gagatcgcgg
     4621 gtcaccatgc gcgcggtccg cggatcgcgc accgagtgct cgtggagcgc ctggccgatg
     4681 ccccacacca tgccgccgag cagctggctg ctggcggtct tggggttgag gatccggccg
     4741 gcggcgaacg cgccgaccag acgatcgacc cggatctcgc cgaggtcctc gtcgacccgg
     4801 accttggcga acaccgcgcc gaacccaccg accgagtact gatcgcggtc cttgtgggcc
     4861 tcggtgtgga gctcgacgct gatctcgggc tgcttggacc gggcgacgat cgccgcgtag
     4921 gggtcgcggc gcttgcgatc ggccttggcg atcagcgcgc cgtcctcggc gtcgatcgcg
     4981 tcgggcgcca ggccgtgcag cggcgacgcg gggtcgccga ccgcctgctc gatcagcttc
     5041 ttgcgcagcg cgacgcacgc gttgtggacg gcggggccgg tgctcgccgc ggtgaacgag
     5101 ccgaccgacg gcggggcctc gggcagcgcg gtgtcgccga gctcgaaccg gatatcgccg
     5161 atcgggagcc cgagggcgtc gccggcgatc tgggtcatga ccgtgtaggt gccggtgccg
     5221 atgtcctggg tgccggcctg cacgagcgcg cggccgtcgg ggcgcatggt agcgcgcgcc
     5281 gacgccggca gctggcgcga cccccgggtg gtggtggcga cgccgtagcc gatctgccag
     5341 cggccctcgc gcagcgtctt gatctgcgcg ggccgcttcg accagccgat cgcctcggcg
     5401 gcgcgctggt agcactcgag cagcgacttg ctcgaccacg gcttgccggt ctccggatcg
     5461 tggtcggcgt gattcttgcg ccggagctcc agcgggtcga tgccggccgc gtgggcgagc
     5521 tcatcgagcg ccgactccag ggcgaacgtg ccgaccgact cgccgggcgc gcgcgtgaac
     5581 gtcggcgtcg gtacgtcgag ccggaccagg cgctggacgg tcgcgacgtt ggggcacgcg
     5641 tagagcatgc gggtgaccgc ggtgcagctc tcgacgaaca cgtcgtagcg cgaggtctcg
     5701 gacaggcagt cgtggcggat cgcggtgagc cggccggcgg cgtcgaggcc gagctcgagc
     5761 ttctgcacgg tcttgggccg gtggccgacc agcgactgca tctgcggccg cgtgatcgcg
     5821 agcttgacgg cgcggctggt cgccttcgca gcgatcgcgg cgagcagcac gtgcgaccac
     5881 ggcgcgccct tgcaaccgaa gccgcctccg acgaagtggt tgatcacccg cacgttctcg
     5941 gccggcaggc cgagcatggt cgacagccgg cggcggacgt tgaagatcgc ctgggtcgag
     6001 tcgtacagcg tcaggtggtc ttcgccctgc cacaccgcga tcgtcgcgtg cggctccatc
     6061 gggttgtggg tctcgaccgg cgtgcggtag gtcgcgtcga tccgggtctt ggcggcggcc
     6121 atcccggcgg agaagtcgcc gcggatgatc tcggcggggc cgcgcggggt ctggaagatg
     6181 tatgccgagc cgaggtcggc ctcgacgtcg ccgaccggcg gggccgcgtc gtactcggcg
     6241 gtgaccagcg ccgcggcgtg ctgagcgcgc tcgagcgtat cggcgaccac cacggcgatc
     6301 ggctggtcgt ggtagagcac gacgctgtcc tgcaggccct ggacgacccg gtcgttggcc
     6361 tcgtggttgc cactcacgcc ggggaacttc ggcgcgttgt cgtgggtgac caccgcgagc
     6421 acgccgggcg cggcctgggc ggcggcggtg tcgatcgcct tgatcttgcc gcggccgatc
     6481 gtgctgccga cgatcaccgc gtaggtgacg ttggcgacct ggacctcggc ggcgtaggtc
     6541 gcaccgccgg tgactttctt gcggccatcg acgcggtcga tgccggttcc gatgagcgga
     6601 cgggtcatgc tgccccccac gcgagctcga gggctcgcac gatcgtgcgc ttggccaggg
     6661 cgatcttgaa cgcgttgtcg cggcgcggtt tcggatcgac gatcgccgcc gccgcggcgc
     6721 gctcgaagct cgtgcggttc ggggcctgac cgaccagcgc atgctcggcc tcgaccgcgc
     6781 gccacggcct ggtcgcgacc ccgccgagcg cgatccgcgc ggcgcggatc ttgccgtcgg
     6841 cgacgtcgag cccgaccgcc gccgacgcga gggcgaacgc gaacgccgcc cggtcgcgga
     6901 ccttgacgta gtgcgcgtgg gcggcgaacg gcgacgccgg cagctcgacg tgggtgacca
     6961 gctcgccggg ctcgagcgcg ttctcgacct cggggtggtc gccgggcagg gtgtggaact
     7021 cgccgaacgg gatcgcgcgc tcgcggccgg cgccgcgggt gtggaccacc gcgtcgagcg
     7081 ccgccagcgc gacgcacatg tccgacgggt gggtcgcgat gcaggactcg ctggtgccga
     7141 ggatcgcgtg cgatcgggtg aagccatcga tcgccgagca gcccgagccg ggcgtgcgct
     7201 tgttgcacgc caccgcgaga tcgcggaagt acgggcagcg cgtgcgctgg agcaggttgc
     7261 cgccgaccgt ggccatgttg cggacctgct gggacgcgcc ggcgagcagc gcctgggcga
     7321 gcagcggata gcgctcgacg accagcgggt gggtcgcgac cgcggagttg cgcgccatcg
     7381 cgccgatccg caggccgtcg cccgcgcgct cgatctgggt cagcggcagc ggcgacaggt
     7441 cgaccaccgc gctcggggtc atgacctcga ggcgcatcag gtcgaccagc gtggtgccgc
     7501 cggccaggaa gctggcgctg ccgtcgctgg cggccgcgat cgccgcgcgc gcgtcggcgg
     7561 gccgcacgta ggtgaagggc ttcacgctag acctccttcc gcgcggcctg gatcgccgcg
     7621 acgatgttgg ggtaggcgcc gcaccggcag atgttgccgc tcatctgctg ccggacctcg
     7681 tcgtcggtct tcgcgcggcc ctcgcggagc agcgcgaccg cgctcaggat ctggcccgac
     7741 gtgcagtagc cgcactggaa cgcgtcgtgg gtgatgaact gggactgcac cgggtggagc
     7801 tggtcgccgc gggcgaggcc ctcgatcgtc gtgatctcgg cgccctcggc ggcgaccgcc
     7861 agggtcaggc acgcgttgac cgcgtggccg tcgcgcagca ccgtgcacgc gccgcactgg
     7921 ccgtgatcgc agcccttgcg cgcgccggtc agcgccaggt gctcgcgcag cgcatcgagc
     7981 agcgaggtcc gcgggtcgat ccgcagccgg cggtcctggc cgttgatccg cagcacgacg
     8041 tcgacctcgc tgccggcctt gaccgcgacc ggcgcgggcg ccggcgggcg atcggcgccg
     8101 ggcgtccggc agccgtggaa caggacgacg ctgaacccgg cgacc
//
LOCUS       DASVOR010001928         2577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_21315,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001928 DASVOR010000000
VERSION     DASVOR010001928.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21315"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..575
                     /locus_tag="VF516_35780"
     CDS             <1..575
                     /locus_tag="VF516_35780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113163.1"
                     /translation="HAAYAGADAEARHTHHAAGSAGTACLACHMPKKNMGLDYALIRY
                     HRIGAPTDPRRVLGDRPVECALCHPDRTVEDLVSTMERWWNKRYDRAALQALYGDDLT
                     VNALRATLARGKPHEQAVAIAVLGQSGDRSAVPAIAAELTHDYPLVRYFAQRALETLT
                     GAPVAIDVGAPAAEVRRAAAGWLTAAAARR"
     gene            complement(550..957)
                     /gene="pth2"
                     /locus_tag="VF516_35785"
     CDS             complement(550..957)
                     /gene="pth2"
                     /locus_tag="VF516_35785"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEX8113164.1"
                     /translation="MADESGAATRTAKQVIVMRKDLGMRKGKMIAQGAHASLKVLLDA
                     GAIEQGAFTLPLDAALTEWLGGRFTKICVSVDSEAALDAVIERARAAGVPCALVVDAG
                     HTEFHGVPTKTCCAVGPAWSDAVDAITGGLPLL"
     gene            1069..>2577
                     /locus_tag="VF516_35790"
     CDS             1069..>2577
                     /locus_tag="VF516_35790"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HEX8113165.1"
                     /translation="MLPSKRFVSSFGMLTGISRLALGVFLGLTPSCALDDGSAADPSV
                     DDGTVADDGEAVSSERVCNGGRVVCHAHVRTFGAERSRAPRAVTPLAVPAGYGPADLQ
                     SAYGIDPTRLATATRPTVAITDAFGYPNLEADLAVYRQTYGLPPCTTANGCLKIMNQQ
                     GQTTNLPPAPPTTDDWTIETALDVDMVSAACPLCNILVVQAVDDQSDGLDIAQNTAAT
                     HGATVISDSWGSPEQKGQSFTASDTTYYNHPGIAIFVASGDAGYDDTIQAPPAAGQPA
                     PPTGPGYPATSQYTIAVGGTRLVKAAGTTRGWTETAWAVTITQGKIDPTRGAGGSGCS
                     LSIPKPAYQSASPCTFKATSDIAAVGDPATGVAVYNSNGGIKGNWGIIGGTSAAAPFV
                     AAIFAATGNGNQTSGKFVADNVGKMFDVTSGNNGTCPTGQGLLCNAAAGWDGPTGFGT
                     PNVKLLMPAATGTGNGNGTGGGTGNGSASASDSNSSDITGGCSAGGGAGLLLG"
BASE COUNT          402 a          955 c          882 g          338 t
ORIGIN      
        1 gccacgccgc gtacgccggc gccgacgccg aggcgcgcca cacccaccac gccgcgggca
       61 gcgccggcac ggcgtgcttg gcgtgccaca tgccgaagaa gaacatgggg ctcgactacg
      121 cgctgatccg ctatcaccgg atcggcgcgc cgaccgaccc gcgccgcgtg ctcggcgacc
      181 ggccggtcga gtgcgcgctg tgccatcctg acaggaccgt cgaggacctg gtgtcgacga
      241 tggagcgctg gtggaacaag cgctacgatc gcgcggcgct gcaggcgctg tacggcgatg
      301 acctgacggt caacgcgctg cgcgccacgc tggcacgcgg caagccgcac gagcaggcgg
      361 tggcgatcgc ggtgctcggc cagtccggcg atcgcagcgc ggtgccggcg atcgccgccg
      421 agctcaccca cgactacccg ctggtccgct acttcgcgca gcgcgcgctc gagaccctga
      481 ccggcgcgcc ggtcgcgatc gacgtcggcg cacccgccgc ggaggttcgc cgcgccgccg
      541 ccggctggct cacagcagcg gcagcccgcc ggtgatcgcg tcgaccgcgt cgctccacgc
      601 cgggccgacg gcgcagcagg tcttggtcgg cacgccgtga aactcggtgt ggccggcgtc
      661 gaccaccagc gcgcacggca cgcccgcggc gcgcgcgcgt tcgatcaccg cgtcgagcgc
      721 ggcctcggag tcgaccgaca cgcagatctt ggtgaaccgg ccgccgagcc attctgtcag
      781 cgcggcgtcg agcggtagcg tgaacgcgcc ttgctcgatc gccccggcgt cgagcagcac
      841 cttgagcgag gcgtgcgctc cctgggcgat catcttgcct ttccgcatcc ccaggtcctt
      901 gcgcatcacg atcacctgct tcgccgtcct ggttgccgca ccggattcat ctgccacagt
      961 gcagcattct agtgccacgg tttggtgatc cgggatgcac gtcgaggagg tcatgtaggg
     1021 cggaagctga cctgtgtggc acgcatgttg attatgcagc agtaccacat gctcccctcc
     1081 aagcgcttcg tgtcgagttt cgggatgttg actggcattt ccaggctcgc cctgggggtt
     1141 ttcctggggc tcactccctc atgcgctctc gacgacggca gcgcagcgga cccctcggtc
     1201 gatgacggca ccgtcgcgga cgacggcgaa gcggtctcgt ccgagcgggt gtgcaatggc
     1261 ggtcgcgtgg tgtgccacgc ccacgtccgg acgttcggcg cggagcgcag tcgagcgccg
     1321 cgcgcggtga cgccgctggc ggtgccggcc ggctacggtc ctgccgatct gcagagcgcg
     1381 tacggcatcg atccgacccg gctcgcgacc gcgaccaggc cgaccgtcgc gatcaccgat
     1441 gcgttcggct atcccaacct cgaggccgac ctcgcggtgt accgccagac ctacggcctg
     1501 ccgccgtgca cgacggccaa cggctgcctc aagatcatga accagcaggg ccagaccacg
     1561 aacctgccgc cggcgccccc caccacggac gactggacga tcgagaccgc gctcgacgtc
     1621 gacatggtca gcgccgcgtg cccgctgtgc aacatcctcg tcgtgcaggc cgtcgacgac
     1681 cagagcgacg gcctcgacat cgcgcagaac accgcggcca cgcatggcgc gacggtgatc
     1741 agcgacagct ggggctcgcc cgagcaaaaa ggccagagct tcacggcgtc ggacaccacc
     1801 tactacaatc accccggcat cgcgatcttc gtggcgtcgg gcgacgccgg ctacgacgat
     1861 accatccagg cgcccccggc cgcggggcag ccggccccgc cgaccggacc ggggtacccg
     1921 gcgacctcgc agtacacgat cgcggtcggc gggacccgcc tggtcaaggc ggccggcacc
     1981 acgcgcggct ggaccgagac cgcgtgggcg gtgacgatca cccagggcaa gatcgacccc
     2041 acccgcggcg cgggcggcag cggctgcagc ctgtcgatcc ccaagccggc ctaccagagc
     2101 gcatcgccgt gcaccttcaa ggcgacctcc gacatcgccg cggtcggcga tccggcgacc
     2161 ggcgtggcgg tctacaacag caacggcggc atcaagggca actggggcat catcggcggc
     2221 accagcgccg cggcgccgtt cgtcgcggcg atcttcgcgg ccaccggcaa cggcaaccag
     2281 acctcgggca agttcgtcgc cgacaacgtc ggcaagatgt tcgacgtcac cagcgggaac
     2341 aacggcacgt gtccgaccgg gcagggcctc ttgtgcaacg cggccgccgg ctgggacgga
     2401 ccgaccggct tcggcacgcc gaacgtcaag ctgctgatgc cggcggcgac cggcacgggc
     2461 aacggcaacg gcaccggcgg cggcaccggc aacggctcgg ccagcgccag cgactcgaac
     2521 agctcggaca tcaccggcgg ctgctcggcc ggcggcggcg cgggcctcct gctcggc
//
LOCUS       DASVOR010001929         2733 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_224845,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001929 DASVOR010000000
VERSION     DASVOR010001929.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224845"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VF516_35795"
     CDS             complement(<1..555)
                     /locus_tag="VF516_35795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113166.1"
                     /translation="MQRAEPERVQGTLDLLGGQAKQVVRGERRRRMRERLDAGDRART
                     DDVAVALAPRIDEPPQCDHAIPVQLLRDLVERVEQEQARPARGDALQGHALARRRRRI
                     DDQLHQIANVVDLHQLRQVDVDRNRRRRVLPRAFRGGRRDLQQGRGLAGAALADDQDA
                     RPVPARELLEVVHLLQRHGRGLGVL"
     gene            complement(891..1370)
                     /locus_tag="VF516_35800"
     CDS             complement(891..1370)
                     /locus_tag="VF516_35800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113167.1"
                     /translation="MNSTSARTATGLLATRLRHEHATTRRRRSSSRSISIQRCSSPAW
                     TAGSTRDAEAIAAAAMTGFRSASASTSSVLSSTQPTIIVKTGTRRWQDTSTGDLVRTS
                     DQTSLGKSSISASSRPAANQHAAKYSWNSWCRNDTRSPACCSTSSATISSRQPSTVQ"
     gene            complement(1488..1745)
                     /locus_tag="VF516_35805"
     CDS             complement(1488..1745)
                     /locus_tag="VF516_35805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113168.1"
                     /translation="MSFQPSMPASAREASAARAASAVSSAIWRSAAMVRARSMVASTA
                     SSLERRASSPPAARQCSNATTARCEIGSCPSTVPIRHSALQ"
     gene            1910..>2733
                     /locus_tag="VF516_35810"
     CDS             1910..>2733
                     /locus_tag="VF516_35810"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8113169.1"
                     /translation="MIADRRIPIGDAVVDALLRDGSVRCRRDLVAVAVDLLGVAGAIA
                     KLVEASRRDRVWLRHLDARALAPLPVGRATADDSISLSIRHQVAIAVASALGGPEGPE
                     EYWVTPSGRDGRSWLVTIHSETRPRYEVCLSPDGLELYDRSMCVTAIRALTDIGPAAL
                     AALRDASERADSKIRSAAITGLARLGDPELADRLLGLLGGTSDPVLVDAALEALGKLL
                     APRAIVLVEDLLGMTEDGSDIHPVWGTCRSRPGWSDAIHRILVGIGADREVAAAIDA"
BASE COUNT          369 a          935 c         1026 g          403 t
ORIGIN      
        1 gagcaccccg agaccgcgac cgtggcgctg caggaggtgc acgacctcga gcagctcgcg
       61 ggccgggacc gggcgcgcat cctggtcgtc ggcgagggcg gcgccggcaa gaccgcgtcc
      121 ttgctgcagg tcgcggcgac cgccgcggaa cgcgcgcggg aggacccggc ggcgccggtt
      181 ccggtctacg tcgacctggc gcagctgatg cagatcgacg acgttcgcga tctggtgcag
      241 ctggtcgtcg atgcgacgcc gccggcgagc gagagcgtgt ccctgcaggg catcgccgcg
      301 cgcaggccgt gcctgttcct gttcgactcg ttcaacgagg tcccggagca gctgcaccgg
      361 aattgcgtgg tcgcactgcg gcggttcgtc gatacgtggg gcgagcgcca ccgctacatc
      421 gtcggttcgc gcgcggtccc cggcatcgag ccgctcgcgc atccgccgtc gccgttcacc
      481 gcgtacaacc tgcttcgcct gtccgccgag caggtccagg gtaccctgga cgcgctcggg
      541 ctcggcgcgc tgcacgagcg gatgcctcag gagctgcggg atctcgcgcg caacccgttc
      601 atgctcgtgg cgatcgcccg gaccctggcg ggcaccgcgg agcacgacct gccgcgcaac
      661 cgcggcaagc tgtaccagcg cgtgctcgag acctggacgg cgaacgaggc ggccaagcgt
      721 cccctcgagt actcctacga gcgcgtcaag gcgccggtgc tgtcctacct cgcggcgcgg
      781 atgaccgcgg ccggtcagac gtcgctgtcg tggcgcgagc tcgagcccga gatcgagcag
      841 ctgctcgagg ccacgcacca gcggatccgg cggctcggcg gaatgcccga tcactggacg
      901 gtggacggct gtcgcgacga gatcgtggcc gacgacgtgc tgcagcaggc cggcgaccgc
      961 gtgtcgttcc ggcaccagct gttccaggag tacttcgccg cgtgctggtt tgcggcgggg
     1021 cgggaggacg cgctgatcga gctcttgccc aggctggtct ggtcgctggt gcgcacgaga
     1081 tcgccggtgg acgtgtcctg ccatcgcctg gttccggtct tgacgatgat ggtcggctgg
     1141 gtcgaggaca gcacggagct cgtcgaggcg ctcgccgagc gaaacccggt catcgccgcg
     1201 gccgcgatgg cctcggcgtc ccgggtcgat cccgcggtcc atgccgggct ggagcagcgc
     1261 tggatcgaga tgctgcgcga tgacgatctg cgccggcgcg tggtcgcgtg ctcgtgcctg
     1321 agcctggtcg cgaggagtcc ggtggcggtg cgggcgctcg tcgagttcac gctcgacggg
     1381 gcaccccaga atacccacgt gggcgcggcg gtgctgccga gggtcaatgc cgtggacgag
     1441 ctggcgaccc agctcgtcga gacgatcctg gcactcgacg aggagcgcta ctggagggcg
     1501 gagtggcgga tcgggaccgt gctcgggcag ctgccgatct cgcacctcgc ggtggtcgcg
     1561 ttcgagcact ggcgcgcggc cggcggcgac gatgctcgcc gctccaggct cgaggccgtg
     1621 ctggccacca tcgaccgcgc gcgaaccatc gcggcgcttc gccagatcgc cgacgacacc
     1681 gccgacgccg cgcgcgcggc cgatgcctcc cgtgcgctcg ccggcatgga tggctggaac
     1741 gacatcgggc tgctgatcgt tcccagctgg atccgtcaac tgccaaagct gtacgacgcg
     1801 agctacgccg cgcagcgcga ccgggaagcc gcccggttga aggaggccga tgtcgcgacg
     1861 ctcggggcag cgctggcccg cggacgcgac gcgtgccggt gcggctcgcg tgatcgccga
     1921 ccggcgcatc ccgatcggcg atgcggtcgt cgacgcgttg ctgcgcgacg gttcggttcg
     1981 gtgccggcga gacctcgtcg cggtcgcggt cgatctgctg ggcgtggccg gcgcgatcgc
     2041 gaagctggtg gaggcctcgc gccgagatcg ggtctggcta cgacacctcg acgcgcgcgc
     2101 gctcgcgccg ctgccggtcg ggagggcgac cgcagacgac agcatcagcc tgagcatccg
     2161 gcatcaagtg gcgatcgccg tggccagtgc gctgggcggg cccgaggggc ccgaggagta
     2221 ctgggtgacg ccgagcggac gggacggtcg gtcctggctg gtgacgatcc acagcgagac
     2281 gcgtccgcgg tacgaggtct gtctctcgcc ggacgggctc gagctctacg atcgctcgat
     2341 gtgcgtgacg gcgatccggg cgctcacgga catcggtccg gcggcgctcg ccgccttgcg
     2401 cgacgcctcc gaacgcgcgg attccaagat tcgaagcgcg gcgatcaccg ggctggcgcg
     2461 actgggagac cccgagctcg cggatcggct gctcgggctg ctcggaggca catcggatcc
     2521 cgtgctggtc gacgccgcgc tcgaggccct cggcaagctc ctggcgcccc gggcgatcgt
     2581 gctcgtcgag gacctgctcg gcatgaccga ggacgggtcc gacatacatc ccgtatgggg
     2641 aacatgccga tcgcgacccg gctggagcga tgccatccat cgcatcctgg tcggcatcgg
     2701 cgccgatcgc gaggtcgcgg cggcgatcga cgc
//
LOCUS       DASVOR010001930         5405 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341202,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001930 DASVOR010000000
VERSION     DASVOR010001930.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5405
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341202"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VF516_35815"
     CDS             <1..900
                     /locus_tag="VF516_35815"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8113170.1"
                     /translation="PGAVTLMQAFLHQLLSGIADGAIYASVALALVMIFKATHQVNFA
                     QGEMAMFSTYIALALTQAGVPYWVAFVLTLVFSFVVGLAIQRLLLRQISRMPHANELT
                     AVVVFIGLLIIFNGAAGSLFGYTLKEFPTPFDPGAWYGSTYLSSHEVGMIAMTLIVLS
                     IVFAFFRFTRLGLAMRAAAENPQSSRLVGIKVGWMLALGWGLAAVLGAVAGMMAAHKV
                     FLDPNMMAGVILYGFAAALLGGIDNPWGAPIGGFLVGVLVNLAGAYVVGSHLQQALAL
                     LVIVAVLVFRPSGLFGRTVVVRV"
     gene            897..1958
                     /locus_tag="VF516_35820"
     CDS             897..1958
                     /locus_tag="VF516_35820"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8113171.1"
                     /translation="MTAVSATAAIAGAPARRISGRMGWLVALLIAATVAPFVLSDYHV
                     FQATQIVVYAIAVLGFNLLTGFSGQISLGNGAFYALGAYITSILMTQYNVPYWATLPV
                     AGAACFGVGFLFGRSATRLEGLYLALATFGLAIAMPQVLKLDALERWTGGSQGVVLNK
                     PVSPIGGLLGDDRWLYFFCLAVAIVLFTAAWNVMRGRTGRAMIALRDHPIAAGTMGIH
                     VPSYKARVFGVSAMYTGIAGALSALVAGFVAPDSFTFLVSIAFLIGGVVGGVASIFGS
                     LFGAAFIELVPDLAKQASELWPSLHWLADVQWPAYGVLLIVTMMVMPGGMAGLIYSVQ
                     LRLAHRAHRAQRAHQPSRS"
     gene            1993..3282
                     /locus_tag="VF516_35825"
     CDS             1993..3282
                     /locus_tag="VF516_35825"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8113172.1"
                     /translation="MSKRNWFIVTTAPRLAALTGVCLILAAGCDKKKDGGDSKPSPLA
                     TDKGGGDSGTPGVTATEIKIGQTMPYSGPASAYGVIGKTEAAYFKWLNDKGGINGRKI
                     NLISLDDGYSPPKTVEQIHKLVESEGVAFTFNTLGTPSNTAIQKYLNEKKVPQLFVAT
                     GADKWGDPEHFPWTMGWQPSYRTEAQIYAKYLLKEKPAAKLCVLYQNDDFGKDYLKGL
                     QEGLGDQYDKIVTKSVSYEVSDPTIDSQVVTLQGAGCDSLLVATTPKFGAQAIRKVAE
                     LGWKPLFFMSNVSVSLTSVLKPAGLDKSTGIISGHYAKDPSDPAMANDPGMKDYLAWA
                     KQNLPSDVDATDLNTVYGYAVSMSLVQVLTACGSDLSRANIMKQAASLSHLKIPVLID
                     GIEVNTSATDFRPIQQMQLRRFNGTNFETFGEVMAGS"
     gene            3529..5130
                     /locus_tag="VF516_35830"
     CDS             3529..5130
                     /locus_tag="VF516_35830"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8113173.1"
                     /translation="MRRILVAVPLVMLAGPAGAQQADDINRVIDQGLNHSQVMQIAEY
                     MDDRIGPRLTNSPQMRQAEAWTQQQFKGWGLRNVRKEAFPFGRGWSIVSSSVRMTAPR
                     VIQLTAIPVAWTPGTGGTITAPVVVAPMKRERDFEAWRGKLAGKIVLVTLPNDGSEPG
                     EAPFKRLSNEDIAKLDRYREPDYDPTSVDRIVKRVTFENKLDGFLKAERAVGWARMAY
                     RDGKLVHGEGRGFRPGATPTLPGLEIAAEDYRRLARLAKVGATPTLEINTAVQYDDSD
                     SNAYNILAEIPGSAGGTEYVMAGAHLDSWVAGDGAADNGAGSAVVMEAARILARLGVK
                     PKRTIRFALWAGEEQGLLGSMAYVEKYLATRAPTSSSESPIETFFRWRYRFPITPRPG
                     YKDLVAYFNIDNGSGKIHGIHTEGNVAATPLFREWLQPFASMGASSVVASPTGGTDHV
                     FMQAVGVPGYQFIQDPLDYGSRVHHTSIDTFDHLKADDMRQASVVLAGILFEAANSAK
                     TLPRMPLPTEGTATNPFKYDDPDDN"
     gene            complement(5127..>5405)
                     /locus_tag="VF516_35835"
     CDS             complement(5127..>5405)
                     /locus_tag="VF516_35835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase"
                     /protein_id="HEX8113174.1"
                     /translation="PVETQFLMAELDTVTDLTMSQPMVTADQVAALVVRCALDGKLER
                     ATPRLSSALTTAGYLAPGLRRVLRPMLERRGRRAKQKLRERRAGDEPG"
BASE COUNT          850 a         1899 c         1823 g          833 t
ORIGIN      
        1 cctggggcag tgaccctcat gcaggccttc ctccatcagc tgctgtccgg catcgccgac
       61 ggcgcgatct acgccagcgt cgcgctcgcg ctggtgatga tcttcaaggc gacccaccag
      121 gtgaacttcg cgcagggcga gatggcgatg ttctcgacct acatcgcgct ggcgctgacc
      181 caggccgggg tcccgtactg ggtcgcgttc gtgctgaccc tggtgttctc gttcgtcgtc
      241 gggctcgcga tccagcgcct gctgctgcgc cagatcagcc ggatgccgca cgccaacgag
      301 ctgaccgcgg tggtcgtgtt catcggcctc ttgatcatct tcaacggcgc ggcgggctcg
      361 ctgttcggct acacgctcaa ggagttcccg accccgttcg atccgggcgc ctggtacggc
      421 tcgacgtacc tgtcgtcgca cgaggtgggg atgatcgcca tgacgctgat cgtgctctcc
      481 atcgtgttcg cgttcttccg gttcacccgg ctcgggctcg cgatgcgagc ggcggcggag
      541 aacccgcagt cgagccggct ggtcgggatc aaggtcggct ggatgctcgc gctgggctgg
      601 ggcctggccg cggtgctcgg cgcggtcgcc gggatgatgg cagcccacaa ggtgttcctc
      661 gatcccaaca tgatggccgg ggtgatcctg tacggcttcg ccgccgcgct gctcggcggg
      721 atcgacaacc cgtggggcgc gccgatcggc ggcttcctcg tcggcgtgct ggtcaacctc
      781 gccggcgcct acgtcgtggg cagccacctc cagcaggcgc tcgcgctgct ggtgatcgtc
      841 gcggtgctgg tgttcaggcc gagcgggctg ttcggccgga ccgtggtggt gcgcgtatga
      901 cggccgtcag cgcgaccgcg gcgatcgccg gcgcgccggc gcggcggatc agcgggcgga
      961 tgggctggct ggtggcgctg ctgatcgccg ccaccgtggc gccgttcgtg ctcagcgact
     1021 accacgtgtt ccaggcgacc cagatcgtgg tctacgcgat cgcggtgctc gggttcaacc
     1081 tgctgaccgg gttcagcggc cagatctcgc tgggcaacgg ggcgttctac gcgctcgggg
     1141 cctacatcac gtcgatcctg atgacgcagt acaacgtgcc gtactgggcg accctgccgg
     1201 tcgccggcgc ggcgtgcttc ggcgtcggct tcctgttcgg ccgctcggcg acccgcctcg
     1261 agggcctgta cctcgcgctc gccaccttcg gcctggcgat cgcgatgccg caggtcctca
     1321 agctcgatgc gctggagcgc tggaccggcg gcagccaggg cgtcgtgctg aacaagccgg
     1381 tgtcgccgat cggcggcttg ctcggcgacg atcgctggct gtacttcttc tgcctggcgg
     1441 tcgcgatcgt gctgttcacc gcggcgtgga acgtgatgcg gggccgcacc ggccgggcga
     1501 tgatcgcgct gcgcgatcac ccgatcgccg cggggacgat gggcatccac gtcccgtcgt
     1561 acaaggcgcg ggtgttcggg gtcagcgcga tgtacaccgg gatcgccggc gcgctgagcg
     1621 cgctggtcgc cggcttcgtc gcgcccgaca gcttcacgtt cctggtgtcg atcgcgttcc
     1681 tgatcggcgg cgtggtcggc ggcgtcgcgt cgatcttcgg ctcgctgttc ggcgccgcgt
     1741 tcatcgagct ggtccccgac ctggccaagc aggcatcgga gctgtggccg tcgctgcact
     1801 ggctcgccga cgtgcaatgg cccgcgtacg gcgtgctgct gatcgtcacc atgatggtga
     1861 tgcccggcgg catggccggg ctgatctact cggtccagct ccgcctcgcc caccgggccc
     1921 accgggccca gcgcgctcac caaccatcca gatcttgacc gggctctcgc ggtgtcacaa
     1981 aacggaggat acatgtcaaa gcggaactgg ttcatcgtca cgactgcgcc ccggctcgcg
     2041 gcattgaccg gggtctgcct catcctggcc gccggctgcg acaagaagaa ggacggcggc
     2101 gactccaagc cttcgcccct tgccaccgac aagggcggcg gcgactcggg cacgccgggc
     2161 gtcacggcga ccgagatcaa gatcggccag accatgccgt acagcgggcc ggcgtcggcc
     2221 tacggcgtga tcggcaagac cgaggccgcc tacttcaagt ggctcaatga caagggcggc
     2281 atcaacggcc gcaagatcaa cctgatctcg ctcgacgacg gctacagccc gcccaagacc
     2341 gtcgagcaga tccacaagct ggtcgagagc gagggcgtcg cgttcacgtt caacacgctc
     2401 ggcacgccga gcaacaccgc gatccagaag tacctcaacg agaagaaagt gccgcagctg
     2461 ttcgtcgcga ccggcgcgga caagtggggc gatcccgagc acttcccgtg gacgatgggc
     2521 tggcagccga gctaccggac cgaggcccag atctacgcca agtacctgct gaaggagaag
     2581 ccggcggcca agctgtgcgt cctctaccag aatgacgact tcggcaagga ctacctgaag
     2641 ggtctgcagg aagggctcgg cgatcagtac gacaagatcg tgaccaagag cgtgtcctac
     2701 gaggtcagcg atcccaccat cgactcgcag gtcgtgacgc tgcagggcgc gggttgcgac
     2761 tcgctgctgg tggccaccac gcccaagttc ggcgcccagg cgatccgcaa ggtcgccgag
     2821 ctgggctgga agccgctgtt cttcatgtcg aacgtctcgg tgtcgctgac ctcggtgctc
     2881 aagcccgccg gactcgacaa gagcaccggc atcatcagcg gccattacgc caaggatccc
     2941 agcgaccccg cgatggccaa cgaccccggt atgaaggact acctcgcgtg ggccaagcag
     3001 aacctgccgt ccgacgtcga cgccacggac ctcaacacgg tgtacggcta cgccgtctcg
     3061 atgtcgctgg tccaggtgct gaccgcgtgc ggcagcgatc tgtcgcgcgc caacatcatg
     3121 aagcaggcgg cgagcctgag ccacctgaag atccccgtgc tgatcgacgg catcgaggtc
     3181 aacaccagcg cgaccgactt ccggccgatc cagcagatgc agctgcggcg gttcaacggc
     3241 accaacttcg agacgttcgg cgaggtgatg gcgggcagct gatcgtccgc ccccggcgga
     3301 tccggaccgt gcgcgccgcg atccgccacc cgatccagac ccttgtgagc cagcggcact
     3361 gcatgggtgt cgcggaggtg tcgcgccccc ggcgagtcga ttcgcagcat cccgcggcat
     3421 cgcgcggtgg atgcgcggcg cgacacacgt gatatatcgg cgtcgagccg gccgcgatcg
     3481 gaagtccgac ccgccgagtg gcgaaccggc ccaaggagag tctgaaacat gcgcaggatt
     3541 ctcgtcgccg tgcctctcgt catgctcgcc ggtccggccg gcgcgcagca agccgatgac
     3601 atcaaccgcg tcatcgatca gggcctcaac cactcgcagg tcatgcagat cgccgagtac
     3661 atggacgacc ggatcggccc ccggctgacc aactcgccgc agatgcggca ggccgaggcc
     3721 tggacgcagc agcagttcaa gggctggggc ctgcgcaacg tccgcaagga ggcgttcccg
     3781 ttcggccgcg gctggtcgat cgtgtcgtcg agcgtgcgga tgaccgcgcc gcgcgtcatc
     3841 cagctcaccg cgatcccggt cgcgtggacg ccgggcacgg gcggcacgat caccgcgccg
     3901 gtcgtcgtcg cgccgatgaa gcgcgagcgc gacttcgagg cctggcgcgg caagctcgcc
     3961 ggcaagatcg tcctggtcac cctgcccaac gacggcagcg agccgggcga ggccccattc
     4021 aagcggctga gcaacgagga catcgccaag ctcgacaggt accgcgagcc cgactacgac
     4081 ccgacgtcgg tcgaccgcat cgtcaagcgg gtcacgttcg agaacaagct cgacggcttc
     4141 ctcaaggccg agcgcgcggt cgggtgggcg cgcatggcct accgcgacgg caagctggtg
     4201 cacggcgagg gccgcggctt ccgccccggc gcgaccccga cactgccggg tctggagatc
     4261 gccgccgagg actaccgtcg gctcgcccgg ctggccaagg tcggcgccac cccgaccctc
     4321 gagatcaaca cggcggtcca gtacgacgac agcgattcca acgcctacaa catcctcgcc
     4381 gagatcccgg gcagcgccgg gggcaccgag tacgtcatgg ccggcgccca cctcgacagc
     4441 tgggtcgccg gcgacggcgc cgccgacaac ggcgcgggct ccgcggtggt gatggaggcc
     4501 gcgcggatcc tggcgaggct cggcgtcaag cccaagcgca cgatccggtt cgcgctgtgg
     4561 gccggcgagg agcagggcct gctcggctcg atggcctacg tcgagaagta cctggcgacc
     4621 cgcgcgccga cgtcgagcag cgagtctccg atcgagacct tcttccgctg gcgctaccgg
     4681 ttcccgatca cgccgcggcc cggctacaag gacctcgtcg cctacttcaa catcgacaac
     4741 ggctcgggca agatccacgg catccacacc gagggcaacg tcgccgcgac cccgctgttc
     4801 agggagtggc tgcagccgtt cgccagcatg ggcgcgagca gcgtggtcgc gtcgccgacc
     4861 ggcggcaccg atcacgtgtt catgcaggcg gtcggcgtcc cgggctacca gttcatccag
     4921 gatccgctcg actacggctc gcgcgtccac cacaccagca tcgatacctt cgaccacctc
     4981 aaggccgacg acatgcgcca ggcctccgtc gtgctcgccg gcatcctgtt cgaggccgcc
     5041 aacagcgcca agaccctgcc gcgcatgccg ctgccgaccg agggcacggc gaccaacccg
     5101 ttcaagtacg acgaccccga cgacaactag cccggctcat cacccgcccg gcgctcgcgc
     5161 agcttctgct tggcgcgccg gccgcgccgc tccagcatcg gccgcagcac gcggcgcagc
     5221 ccgggcgcca ggtagcccgc cgtggtcagc gcgctcgaca gccgcggcgt cgcgcgctcc
     5281 agcttgccgt cgagcgcgca ccgcacgacc agcgccgcga cctggtcggc ggtgaccatc
     5341 ggctgcgaca tcgtcagatc ggtcacggtg tcgagctcgg ccatgaggaa ctgggtctcg
     5401 accgg
//
LOCUS       DASVOR010001931         3572 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_78719,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001931 DASVOR010000000
VERSION     DASVOR010001931.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78719"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            152..904
                     /locus_tag="VF516_35840"
     CDS             152..904
                     /locus_tag="VF516_35840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8113175.1"
                     /translation="MVTARAPQPPVEQAEPAPAILTARDLVKRYRMGEVDVIALRGVD
                     VDLLEGELMVLLGASGSGKSTLLNILGGLDVCTTGRVVYRGMDVSAADEDELTLYRRH
                     HIGFVFQFYNLLPSLTARENVELVTEIADDPMPAAEALELVGLGGRLDHFPAQLSGGE
                     QQRVAVARAVAKRPDILLCDEPTGALDFETGVRVLAALERVNRELGTTTVIITHNAPI
                     AQIAQRVITLADGRIVRTVVNPAPRPVQELRW"
     gene            901..3261
                     /locus_tag="VF516_35845"
     CDS             901..3261
                     /locus_tag="VF516_35845"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8113176.1"
                     /translation="MSALDHKLGRDLWRLKGQVATIALVLACGIMAMVMLRSTWQSLL
                     GARDTYYEQYRLGDVFAHLERAPDAELSRLERLPGVALAYPRVSEDVMVALPGEPDPV
                     SGRLVSIPDAGAPPLGALYLRAGRLPQDGADEAVVIEQFAEAHHLAPGDRLPVVINGK
                     LRALHLVGVALSPEYVLAMSGREVVVDNARFAVLWMRRGAIAPAFRMDGAFNDVVLRL
                     EPGASLQGVLDAVDRELVRYGGRHAVPRARQTSNYALSSELAILRTLALMIPAMFLAV
                     AAFLVNVVVSRLVFLERTQIAVLKALGYADRRIARHYLVLVALIVALAAVLGVALGVW
                     SGRWMTNLYADFYRFPTRLFQVAPSMVALTVGIGLVTAVAGALTAVRRIARMPPAQAM
                     RPPAPLSYRHSVIERLGLHRAVGPAAMMVVREIQRRPFRFALSALGIAMGIAIFVMGR
                     FSWDSFDHLMAEVFPREHQEDITVTLAHALPARALREIEHLPGVELAEGQRVVPVRLR
                     AASRWRDTMIIGLPARPELRHLLDHGTTPLTLPAEGLVMTDRLADLLGVQIGERVDVD
                     VLEGDYATRRLPLAGVLDEAFGMQAYARTDWLARVLDEEPRVSALLLRVDADRSDRVR
                     ARLQQLPEVLGSTTTARIIERYREQTGSSMLVMTLILTLSAAAIAIGVVYNNARIALS
                     MRSRDLASLRVLGFTRVEISGILLGELAAQVVIGIPLGFVLGHWWAAVLAASIDPEAI
                     RFPLHIASSTYAQAGAIGLVSAAVSALLVRRQLDHLDLVGVLKSSE"
     gene            3266..>3572
                     /locus_tag="VF516_35850"
     CDS             3266..>3572
                     /locus_tag="VF516_35850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113177.1"
                     /translation="MSTQPTVSPVPPLVPGTLHPPAHRRHRALSRWLSRIGLVAGGLA
                     IAGGIVRAWLPQPVAVDVAAVRHGPLDVEVDEDGRTQVRDRYAVAAPITGDLQRIELD
                     "
BASE COUNT          421 a         1313 c         1309 g          529 t
ORIGIN      
        1 gcgaccaagc cgcggcgctg agccgcgcgc cgtgcgtcgc ccgcagacgc gcagcggttg
       61 cgcccccggg ggtgcgccgc gcaacgactt cgcgcgccgc ggccggtgaa acgcgcgttc
      121 tcggggcacg gtcgatgcag cgttgccggt gatggtgacc gcacgcgcac cccagccccc
      181 ggtcgagcag gcggagccgg caccggcgat cctcaccgcg cgcgacctgg tcaagcgcta
      241 ccggatgggc gaggtcgacg tgatcgcgct gcgcggggtc gatgtcgacc tactcgaggg
      301 cgagctcatg gtgctgctcg gcgcgtcggg cagcggcaag tcgacgctgc tcaacatcct
      361 cggcgggctc gacgtgtgca ccaccggccg cgtggtgtac cgcgggatgg acgtcagcgc
      421 cgccgacgag gacgagctga cgctgtaccg ccgccaccac atcggcttcg tgttccagtt
      481 ctacaacctg ctgccgagcc tgaccgcgcg cgagaacgtc gagctggtca ccgagatcgc
      541 cgacgacccg atgccggcgg ccgaggcgct cgagctggtc gggctcggcg ggcggctcga
      601 tcacttcccg gcccagctgt cgggcggcga gcagcagcgc gtcgcggtcg cccgcgcggt
      661 cgccaagcgg cccgacatcc tgctgtgcga cgagccgacc ggcgcgctcg acttcgagac
      721 cggcgtccgg gtgctcgccg cgctcgagcg ggtcaaccgc gagctcggca cgacgacggt
      781 gatcatcacc cacaacgcgc cgatcgccca gatcgcgcag cgcgtgatca cgctcgccga
      841 cgggcggatc gtccggaccg tggtcaaccc ggcgccgcgc ccggtccagg agctgcgctg
      901 gtgagcgcgc tcgaccacaa gctcggccgc gatctgtggc ggctcaaggg ccaggtcgcg
      961 accatcgcgc tggtgctggc gtgcgggatc atggcgatgg tcatgctgcg cagcacctgg
     1021 cagtcgttgc tcggtgcgcg cgatacctac tacgagcagt accggctcgg cgacgtgttc
     1081 gcgcacctcg agcgcgcccc cgacgccgag ctgtcgcggc tcgagcggct gcccggcgtc
     1141 gcgctggcct acccccgggt gtccgaggac gtcatggtcg cgctgcccgg cgagcccgat
     1201 ccggtcagcg gccggctggt gtcgatcccc gacgccggcg cgccgccgct cggcgcgctg
     1261 tacctgcggg ccgggcggct gccgcaggac ggcgcagacg aggcggtcgt gatcgagcag
     1321 ttcgccgagg cccaccacct cgcgcccggc gatcgcctgc cggtggtgat caacggcaag
     1381 ctgcgcgcgc tccacctggt cggcgtcgcg ctgtcgcccg agtacgtgct cgcgatgtcg
     1441 ggccgcgagg tcgtggtcga caacgcgcgg ttcgccgtgc tgtggatgcg gcgcggtgcg
     1501 atcgcgccgg cgttccgcat ggacggcgcg ttcaacgacg tggtgctgcg gctcgagccc
     1561 ggcgcgtcgc tgcagggcgt gctcgacgcg gtcgatcgcg agctcgtgcg ctacggcggg
     1621 cgccacgcgg tgccgcgcgc ccggcagacc tcgaactacg cgctgtcgag cgagctcgcg
     1681 atcctgcgca cgctcgcgct gatgatcccg gcgatgttcc tggcggtcgc cgcgttcctg
     1741 gtcaacgtcg tggtgtcgcg gctggtgttc ctcgagcgca cccagatcgc ggtgctcaag
     1801 gcgctgggct acgccgaccg gcggatcgcg cgccactacc tggtgctggt cgcgctgatc
     1861 gtcgcgctcg cggcggtgct cggcgtcgcg ctcggcgtgt ggtcggggcg gtggatgacc
     1921 aacctgtacg ccgatttcta ccggttcccg acccggctgt tccaggtggc gccgtcgatg
     1981 gtcgcgctga ccgtcgggat cggcctggtg accgcggtcg ccggcgcgct gaccgcggtg
     2041 cgccggatcg cgcggatgcc gccggcccag gcgatgcggc cgcccgcgcc gctgtcctac
     2101 cgccactcgg tgatcgagcg gctcggcctg caccgcgcgg tcggtccggc cgcgatgatg
     2161 gtggtccgcg agatccagcg ccggccgttc cggttcgcgc tgtcggcgct cggcatcgcg
     2221 atggggatcg cgatcttcgt gatgggccgg ttctcgtggg actcgttcga tcacctgatg
     2281 gccgaggtgt tcccgcgcga gcaccaggag gacatcacgg tgacgctcgc ccacgcgctg
     2341 ccggcgcgcg cgctgcgcga gatcgagcac ctgccagggg tcgagctcgc cgagggccag
     2401 cgcgtggtgc cggtgcggct gcgcgccgca tcgcgctggc gcgacacgat gatcatcggg
     2461 ctgccggcgc ggcccgagct ccgccacctg ctcgaccacg gcacgacacc gctgaccctg
     2521 ccggccgagg gcctggtgat gaccgatcgg ctcgccgacc tgctcggcgt ccagatcggc
     2581 gagcgggtcg acgtcgacgt cctcgagggc gactacgcga cccgccggct gccgctcgcc
     2641 ggcgtgctcg acgaggcgtt cggcatgcag gcctacgcgc gcaccgactg gctcgcccgc
     2701 gtgctcgacg aggagccgcg ggtgtcggcg ctgctgctcc gggtcgacgc cgatcgcagc
     2761 gaccgggtgc gggcccggct ccagcagctg cccgaggtgc tcggctcgac caccaccgcg
     2821 cgcatcatcg agcgctaccg cgagcagacc ggcagctcga tgctggtgat gacgctgatc
     2881 ctgaccctgt cggccgcggc gatcgcgatc ggcgtggtct acaacaacgc gcggatcgcg
     2941 ctgtcgatgc gcagccgcga cctcgccagc ctgcgcgtgc tcgggttcac ccgggtcgag
     3001 atctccggca tcctgctcgg cgagctcgcc gcgcaggtcg tgatcgggat cccgctcggc
     3061 ttcgtgctcg gccactggtg ggccgccgtg ctggccgcga gcatcgaccc cgaggcgatc
     3121 cggttcccgc tccacatcgc gagctcgacc tatgcccagg ccggggcgat cggcctggta
     3181 tcggcggcgg tcagcgcgct gctcgtgcgc cgacagctcg accacctcga tctcgtcggg
     3241 gtcctcaagt cctcggagtg atgccatgtc cacccagccg accgtctcgc ccgtcccgcc
     3301 cctggtcccc ggtaccctcc accctccggc gcatcgccgg caccgcgcgc tgagccggtg
     3361 gctgtcgcgg atcggcctgg tcgccggcgg cctggcgatc gcgggcggca tcgttcgcgc
     3421 ctggctgccc cagccggtgg cggtcgatgt cgccgccgtg cggcatggcc cgctcgacgt
     3481 cgaggtcgac gaggacggcc ggacccaggt ccgcgaccgc tacgcggtgg cggcgccgat
     3541 caccggcgac ctgcagcgga tcgagctcga tc
//
LOCUS       DASVOR010001932         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_516578,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001932 DASVOR010000000
VERSION     DASVOR010001932.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_516578"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="VF516_35855"
     CDS             complement(<1..1844)
                     /locus_tag="VF516_35855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8113178.1"
                     /translation="MAVTAIQAPAAYGGGGGGGGGGGGGGGGGGGGSGGGSGGGGGGG
                     GGGGSANEKALVVSPHDKGLQTRPPPSDQTSWKSYLGVLRYSRRAIRLVWATNRALAI
                     GLAVGSVVAGVLPGAIAWVGKILINAIVAAAQTHTAADRAAAVAFAVGWLAAELGLVI
                     GRAVVERLLGMMRSLLRQQLGQKINVEILEKALTLELTQFEDSELYDSLTRARREASS
                     RPLSLVSQTFELGQSLITLVTLGGLLARFSWIALAVLIAAAIPPFVAELKFSGDAFRL
                     SRWRTPETREQLYVETVLAREDHAKEVKLFGLGPRFLDRYKAIFEKLYAGDRRITIHR
                     AIWAVSLGAIGTLAFYGMYLWIALETMYGNLSIGEFTMYALVFKSAQSAMASALGDIG
                     GMYEDNLYLSNLYEFLDTPTLAPGGTAMTGPKPGDGVRFEGVSFTYPDARQPALSGID
                     LHIPPGSKLAIVGENGSGKTTLIKLLTRLYEPTEGRITLDGSDLREWAPGALHRRIGV
                     IFQDFVRYQLKVGENIGAGDDRAYDDRARWADAAERGLAAPIIDALPDRYDTQLGKWF
                     KNGRELSLGQWQKVALARSFMRRDADVLVLDEPTASMDAAAEVAIFER"
     gene            1942..2319
                     /locus_tag="VF516_35860"
     CDS             1942..2319
                     /locus_tag="VF516_35860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113179.1"
                     /translation="MGQEREESQGYFEMLWDCDHCDARGLFAKSQRYCANCGAPQNPD
                     KRYFPQPGQEQRVDGHIYEGADRTCPACSAPQSARAKNCTHCGSVLDGAAEVRGFTDA
                     PAAPRAESLRRRGRSTRCGSPAP"
BASE COUNT          351 a          864 c          830 g          347 t
ORIGIN      
        1 aaccgctcga agatcgcgac ctcggcggcg gcgtccatcg acgcggtcgg ctcgtcgagc
       61 accagcacgt cggcgtcgcg ccgcatgaac gagcgggcga gcgcgacctt ctgccactgg
      121 ccgagcgaca gctcgcggcc gttcttgaac cacttgccga gctgggtgtc gtagcgatcg
      181 ggcagggcgt cgatgatcgg ggcggccagg ccgcgctcgg cggcgtcggc ccagcgcgcg
      241 cggtcgtcgt aggcgcggtc gtcgccggcg ccgatgttct cgccgacctt gagctggtag
      301 cgcacgaagt cctggaagat gacgccgatc cggcggtgca gcgcgccggg cgcccactcg
      361 cgcaggtcgc tgccgtcgag cgtgatccgg ccctcggtcg gctcgtacag ccgggtcagg
      421 agcttgatca gcgtggtctt gccgctgccg ttctcgccga cgatcgcgag cttcgagccc
      481 ggtgggatgt gcagatcgat cccggacagc gcgggctggc gcgcgtcggg gtaggtgaac
      541 gacacgccct cgaaccggac gccgtcgccg ggcttcggcc cggtcatcgc ggtgccgcca
      601 ggcgcgaggg tcggcgtgtc gaggaactcg tagaggttgg acaggtacag gttgtcctcg
      661 tacatgccgc cgatgtcgcc gagcgcgctc gccatcgccg actgggcgga cttgaacacg
      721 agcgcgtaca tggtgaactc gccgatcgag aggttgccgt acatggtctc gagcgcgatc
      781 cacaggtaca tgccgtagaa cgcgagcgtg ccgatcgcgc ccaggctcac cgcccagatc
      841 gcgcggtgga tcgtgatccg gcggtcgccc gcgtacagct tctcgaagat cgccttgtag
      901 cggtcgagga accgcggccc gaggccgaac agcttgacct ccttggcgtg gtcctcgcgc
      961 gcgagcacgg tctcgacgta gagctgctcg cgggtctccg gggtccgcca gcgcgacagc
     1021 cggaacgcgt cgcccgagaa cttgagctcc gcaacgaacg gcgggatcgc ggcggcgatc
     1081 agcacggcga gcgcgatcca cgagaaccgc gcgagtagcc cgccgagcgt gaccagcgtg
     1141 atcaggctct gaccgagctc gaaggtctgc gagaccagcg acagcggccg cgacgacgcc
     1201 tcgcggcgcg cccgggtcag gctatcgtac agctcggagt cctcgaactg cgtgagctcc
     1261 agggtcagcg ccttctcgag gatctcgacg ttgatcttct ggccgagctg ctggcggagc
     1321 aggctgcgca tcatgccgag cagccgctcg accaccgccc ggccgatcac gaggccgagc
     1381 tcggcggcca gccaaccaac ggcgaacgcg acggcggcgg cgcgatcggc ggcggtatgg
     1441 gtctgggccg ccgcgacgat cgcgttgatc aggatcttgc caacccacgc gatcgcgccg
     1501 ggcagcacgc cggccacgac gctgccgacc gccaggccga tcgcgagcgc gcggttggtc
     1561 gcccacacca gccggatcgc ccgccggctg taccgcagca cgccgaggta gctcttccag
     1621 ctcgtctggt cggacggcgg cggccgggtc tgcaggccct tgtcgtgcgg gctgaccacc
     1681 agcgccttct cgttcgcgct accgccgccg ccaccaccgc caccaccgcc gccgcttcct
     1741 cctccgcttc ctcctccgcc gcctccgccg ccaccgccac cgccgccgcc tccaccccca
     1801 ccgccgccgt acgccgccgg tgcctggatc gcggtcactg ccatgcctgg actgtagtgc
     1861 ctcctcgccg gctgcgccta cccgccggac gggtagcttt ggtcacgcgc ggccccgccg
     1921 gctgggctac catccggtgc gatggggcaa gaacgcgagg agagccaggg ctacttcgag
     1981 atgctctggg actgcgatca ctgcgacgca cgcggtctgt tcgccaagag ccagcgctac
     2041 tgcgccaact gcggcgcgcc gcagaacccc gacaagcgct acttcccgca gccgggccag
     2101 gagcagcggg tcgacggcca catctacgag ggcgcggacc ggacctgtcc ggcgtgcagc
     2161 gcgccgcagt cggcgcgcgc gaagaactgc acgcactgcg gctcggtgct cgacggcgcc
     2221 gccgaggttc gtggcttcac cgacgccccg gccgcgccgc gcgccgagtc gcttcgtcgg
     2281 cggggaagaa gcactcgatg cggatctcct gcaccgtgat gtcctgtggc gttccgagcg
     2341 tggtcactgt cgagaagaag ctcatctcca gggccgcctt gcgaaactgc ac
//
LOCUS       DASVOR010001933         1768 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_108840,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001933 DASVOR010000000
VERSION     DASVOR010001933.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1768
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108840"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..580)
                     /locus_tag="VF516_35865"
     CDS             complement(89..580)
                     /locus_tag="VF516_35865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113180.1"
                     /translation="MLDPHRKIQALIDSFVADLGEIAKQIALEQLKTAFGSVKLAPLP
                     PPSSYSSSSSSSSSSSSSSAAARRGRGRRAQHEIEALRNKLLTVISDNPGCRAEDINS
                     ALGTRTPQIAQPLRKLVADRLVRTEGARRGTRYFAVGSTDAQNGRRPAASEAVASADE
                     PPA"
     gene            complement(963..>1768)
                     /locus_tag="VF516_35870"
     CDS             complement(963..>1768)
                     /locus_tag="VF516_35870"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8113181.1"
                     /translation="AGYPQLPSHALHPNALHPTALHPNALHPNALHPNALHPNALHPN
                     ALHPSALSPNALYGLQAPPQPASLTGQLRWSEVDEIPSQYKIGAARRRWFTYIVSGLI
                     AVSVAAGATFLVIRLSREATPTTGSVRVVSVPTGADVTFDGTLLSERTPVTIEVVPVA
                     SRHKIVVSLRHYQPYTEEVSIPKTGGTVQMVAQLTSLTGKIVINTTPGGAQIWINGQP
                     RGTTPTTLSGIDIDSTSQIELLHKDFGKYALPLKWDAAGTAYVDYKFSR"
BASE COUNT          247 a          592 c          653 g          276 t
ORIGIN      
        1 cgtcgtccgg cgcgatggtg ttgatcggcg ccgcgctgat cgccggcgtc gcgtcgttcc
       61 tgctcgcgac gctgctgtag ccgcgccgct acgccggagg ctcgtccgcg ctcgccaccg
      121 cctcgctggc ggccggccgg cggccgttct gggcgtcggt ggacccgacc gcgaagtacc
      181 gcgtgccgcg ccgcgccccc tcggtcctca ccagccggtc ggcgaccagc ttgcgcagcg
      241 gctgcgcgat ctgcggcgtc cgcgtgccga gcgcgctgtt gatatcctcg gcccggcacc
      301 ccgggttgtc cgagatcacg gtcagcagct tgttgcgcag cgcctcgatc tcgtgctgcg
      361 cccggcgccc tcggccgcgc cgggccgctg ccgacgagga ggaagaagaa gacgacgaag
      421 aagaagacga cgagtacgag gacggcggcg gcagcggcgc gagcttcacg ctgccgaacg
      481 cggtcttgag ctgttcgagc gcgatctgct tggcgatctc gccgaggtcg gccacaaagc
      541 tgtcgatcag cgcctggatc tttcggtgcg ggtccaacat ccgccccaac ctagctcatc
      601 tccgcacgcg ctgcgcgcga tcgtagcgct ctcgttgcaa gcagcggttg aaccggcgtc
      661 cgccggccgg ctgcggcgcg ggccgtcagg ctggccggta acacgccgag cccgctcggg
      721 acagcctcgt tcagcgacga tgtacgatgc tgccaccgca aaaaaccgcg caccggccat
      781 gtcaccggat ctctgcgtgg ggccgcaaac cagaaattct gtgttccgcg acgacttccg
      841 gtgagcgtca ggacagggcg ccgacgcacg acgagatgtc gaagcagagg aaggcgagcg
      901 ccccgcggtg aagtcgaacg tcacggccat cgccccgtcg ggcagtccgt ggggctccgg
      961 ggtcagcgcg agaatttgta gtcgacgtag gccgtgccgg cggcgtccca cttgaggggg
     1021 agcgcgtact ttccgaaatc cttgtgcagg agctcgatct gggacgtcga gtcgatgtcg
     1081 atcccggaca gcgtggtcgg cgtggtcccg cgcggctggc cgttgatcca gatctgcgcg
     1141 ccgcccggcg tcgtgttgat cacgatcttg ccggtcagcg acgtcagctg cgcaaccatc
     1201 tggaccgtgc cgccggtctt ggggatcgag acctcctcgg tgtacggctg gtagtggcgc
     1261 agcgagacca cgatcttgtg gcggctcgcg accgggacga cctcgatcgt caccggcgtg
     1321 cgctcgctga gcagcgtgcc gtcgaacgtg acgtccgcgc cggtcggcac cgacacgacg
     1381 cgcaccgagc cggtggtcgg cgtcgcctcg cgggacagcc ggatgaccag gaacgtcgcg
     1441 ccggccgcga ccgacaccgc gatcagcccc gagacgatgt acgtgaacca tcgccggcgc
     1501 gccgcgccga tcttgtactg cgacgggatc tcgtcgacct cgctccaccg gagctgcccg
     1561 gtcagcgacg ccggctgggg cggcgcctgg aggccgtaca gcgcgttggg ggacagcgcg
     1621 ctggggtgca gcgcgttggg gtgcagcgcg ttggggtgca gcgcgttggg gtgcagcgcg
     1681 ttggggtgca gcgcgttggg gtgcagcgcg gtggggtgca gcgcgttggg gtgcagcgcg
     1741 tgggagggta gctgcggata gccggccg
//
LOCUS       DASVOR010001934         7464 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_166174,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001934 DASVOR010000000
VERSION     DASVOR010001934.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7464
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166174"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VF516_35875"
     CDS             complement(<1..678)
                     /locus_tag="VF516_35875"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX8113182.1"
                     /translation="MNKKVWIAITVLVWLVALVTWPRRCEQAWFAYGIAVPACPDGEL
                     RQTAELDALELGRGVAGTVALRAFAYYTTRANLDRRAVVPHVRSIALSLTAGKLDRPL
                     DVPAWERDAGAWRAHIALPDVPDGDYQLHARYRTALGDGEVSVPLAVYAPARIHVITD
                     RPLYQPGNTVRFRAVVLRARDLAPLDHRPGSWVVTDPGGEVLLEEAAPAGDWGVVAGS
                     FPLDRAAR"
     gene            complement(912..1169)
                     /locus_tag="VF516_35880"
     CDS             complement(912..1169)
                     /locus_tag="VF516_35880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEX8113183.1"
                     /translation="MSDHPTDFVGSVEDAITSSVKDRIPDAVVAVSGGGGHYRIDVVS
                     AVFAGKSPLERQRLVLSAIKHLINGDRAPVHAVDELITRTP"
     gene            1229..2374
                     /locus_tag="VF516_35885"
     CDS             1229..2374
                     /locus_tag="VF516_35885"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEX8113184.1"
                     /translation="MTDSHAAPAAVRRLERALEAQPLDLALHQQLAEAHRRLLQRADR
                     ERELTAICRELPFAFTSQLHLAALHEARGDRRAAVIGFTRAIKTAQLRGFWHDQGSTP
                     PWILDLVLRAMDIAHHGRVELFHAVLEPLEARYGKDELVRVRRALAMYLGLEPTVYRD
                     PRQRPSFLYIPDLPVAPVFPRDALPFADWYEAQTAAITEEMRAVRDGSGVQPFHYDVP
                     EAQRGQLTAGAWDAYFFYADGERVDAHHAACPRVSAVLGALPLDHVRDHGPEVCFSIL
                     RPGAHILPHRGVTNARAVLHLGLEIPDGCALRLPAVEQLGWQVGRCFAFDDTYEHEAW
                     NRSDTTRTVLLGDIWNPYLRPAEREALAELVGAIGDFNRAATPTARA"
     gene            complement(2466..5294)
                     /locus_tag="VF516_35890"
     CDS             complement(2466..5294)
                     /locus_tag="VF516_35890"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8113185.1"
                     /translation="MSDEELAKLAEQEAKTEVITVTGSLIGRKEVDSPSPVSVVDKEK
                     LQSAGITNVGDVLQKIPAQGNAINAQNNNGGDGSTRINLRSLGTNRTLVLLNGRRVVS
                     SGLGADSSVDFGTIPLAMIERVEVLKDGASAIYGSDAIAGVVNVITRTNMNGTEANVY
                     TATTNHTDGTNYDLSFVTGHSSNRGNITFSGGYQNQKPVMAGDRDFSKATYAYDYSCT
                     PDMQSKGDCIIATLLGSSSSPTGRINTMPNGGPPLNIVGCNTKYCTADGNGGFRNFIS
                     PTATNLGDQYNFQPLNYLLTPSSRVNLFSNGHYDITKNTHVFFEGQFNSRKSEQQLAE
                     EPVQLALFGTPVSADSIYNPFGQDVVDYNRRLVEFGPRTFKQEVNTTRLVVGLNGSID
                     EQVPALKNWKWEGSYNYGRNDSTQATHGDLILSHLGNALGPSFKDAQGPHCGTSSADV
                     IDGCVPLDLLHSGPISQANITPEAINYLTFTGTQSGFNEQHTALLTASGKLLDLPNHG
                     DISLAIGGDYRFERGGQQPDPLTSTGDTTGNAFEPTLGSYHTFEGFGELSVVPISGGE
                     TLKWVEINAAGRAYDYNTFGSGATGKVSGLVRTLGGVAVRGTYGSAFRAPNVAELFSG
                     QADSFPNLADPCDAKPPGKTAPIMLPANVAAQCANPNTSGGGVPTGSQFGTSQQRAKF
                     GGNPALQPEKGTVGTAGLVYEPARGLDFTLDYWHVDITDAITTLPVQTILTQCYQAGD
                     PKFCNQITRDPVTHGISHIFDLIQNVGGVTTSGLDFSAAYQYRNQFGTFRHAFEGTYL
                     FQYNVDTGTKDPDTMKENILHGRGFYDLGVFPDLKFNIFTTWVHPSGVGAGFNFRFID
                     SFKECEQDSCNDPTALRREVSKYATGDVFVSYNLKTSQGTTGITAGMNNVVNVSPPII
                     YNGPVLNADESAYDFLGRQFYIRLSQLF"
     gene            complement(5920..6195)
                     /locus_tag="VF516_35895"
     CDS             complement(5920..6195)
                     /locus_tag="VF516_35895"
                     /inference="COORDINATES: protein motif:HMM:NF019076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113186.1"
                     /translation="MSDSRGKVSTLMETNSDTQTTESETTAPTNRFAELQRLVTGMAT
                     DFEKFYRDGNKAAGTRVRNSMQELKAFAQRVREEVLTLRNQDKEQEQ"
     gene            6298..7095
                     /locus_tag="VF516_35900"
     CDS             6298..7095
                     /locus_tag="VF516_35900"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8113187.1"
                     /translation="MRTTAAAIAALLMCGACGSSKPPEGPRPDDRIKALEAELAKTKD
                     DLEKANAAADELRKTEADLKAKADEATALQAKLAEAVGQSGEVTTADGAVKLELVDQI
                     LFPTGEAELTPNGKDVLSKVGAALKEIGDKQIWVQGHTDDQPIYPRKPKPAEPPPAAK
                     VAKGKKDKAAKPDPRASKPDPKAKPDTKPDTKPDTNDAVLAFVTNWELSAARALTVVH
                     YLQDDAKIDPTRLAAVAFGQYRPISKTKAKNRRIEIVLYPRAKLTPK"
     gene            7187..>7464
                     /locus_tag="VF516_35905"
     CDS             7187..>7464
                     /locus_tag="VF516_35905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113188.1"
                     /translation="MSRTLRVVIAIGVVAIAGAGWWVHARRVQAPAATEASAPSAPGA
                     VRRDRPQRGDPAEFPRMVIDDDPRGTLRLEGQVIDAEDHGVGGATVVIT"
BASE COUNT         1152 a         2506 c         2604 g         1202 t
ORIGIN      
        1 gcgcgcggcc cggtcgagcg gaaacgaccc cgcgactacg ccccagtcac ccgcgggcgc
       61 cgcctcctcg agcagcacct cgccacccgg atcggtgacc acccagctgc ccggccggtg
      121 gtcgagcggc gccaggtcgc gggcgcgcag caccacggcg cggaaccgca ccgtgttgcc
      181 gggctggtag agcggccggt cggtgatcac gtggatccgc gccggcgcgt acaccgccag
      241 cggcacgctg acctcgccgt cgccgagcgc ggtccggtag cgcgcgtgga gctggtagtc
      301 gccgtcgggg acgtcgggca gcgcgatgtg cgcacgccac gccccggcgt cgcgctccca
      361 cgccgggaca tcaagcggcc ggtcgagctt gccggcggtc agcgacagcg cgatcgagcg
      421 cacgtgcggc accaccgcgc gccggtcgag gttcgcgcgg gtcgtgtagt aggcgaacgc
      481 gcgcagcgcg accgtgccgg cgaccccgcg cccgagctcc agcgcgtcga gctcggcggt
      541 ctggcgcagc tcgccgtcgg ggcacgccgg gacggcgatg ccgtacgcga accaggcctg
      601 ctcgcagcgc cggggccagg tcaccagcgc cacgagccac accagcaccg tgatcgcgat
      661 ccagaccttc ttgttcatcg agccagcccc cgctcgcatg agcgtaccag ctagcgacgc
      721 gcaacccgcc cgccgggtct atagagaggg ggctttccca tgatccccct ctgggggatt
      781 tcgcggcaaa gccgcgaaat tcacccctcc gcgcgtcgcg tcgcgcggtt ctgcatcggc
      841 cagcgcacgc aattgtccgt ggccggtgac aaccgccgcg catccccgcg ggatcgcggc
      901 gcggacggcc gtcacggcgt tcgcgtgatc agctcatcga cggcgtggac cggggcgcgg
      961 tcgccgttga tcaggtgctt gatcgccgac agcaccaggc gctggcgctc cagcggcgac
     1021 ttgccggcga acaccgccga caccacgtcg atccggtagt gcccgccgcc gcccgagacc
     1081 gcgacgacgg cgtcggggat ccggtccttc accgacgagg tgatcgcatc ctcgacggag
     1141 cccacgaagt cggtcggatg atcgctcata cgacgtcgtt cgcatgaaac cccgcgccgc
     1201 acaacccggg cgcgctatcc tgggcgcgat gaccgactcc cacgcggcgc cggccgcggt
     1261 acgccgcctg gagcgtgcgc tggaggccca gccgctcgac ctggcgctgc accagcagct
     1321 cgccgaggcc caccgccggc tgctccagcg cgccgaccgc gagcgcgagc tcacggcgat
     1381 ctgtcgcgag ctgccgttcg cgttcaccag ccagctccac ctcgcggccc tgcacgaggc
     1441 gcgcggcgac cggcgcgccg ccgtgatcgg gttcacccgc gcgatcaaga ccgcgcagct
     1501 ccgcgggttc tggcacgacc agggctcgac cccgccgtgg atcctcgacc tggtgctgcg
     1561 cgcgatggac atcgcgcacc acggccgggt cgagctgttt cacgcggtgc tcgagccgct
     1621 ggaggcgcgc tacggcaagg acgagctggt ccgggtccgc cgcgcgctcg cgatgtacct
     1681 gggcctcgag ccgaccgtgt accgcgaccc gcggcagcgg ccgagcttcc tgtacatccc
     1741 ggacctgccg gtcgcgccgg tgttcccgcg cgacgcgctg ccgttcgccg actggtacga
     1801 ggcccagacc gcggcgatca ccgaggagat gcgcgcggtg cgcgacggca gcggcgtgca
     1861 gccgttccat tacgacgtcc cggaggccca gcgcggccag ctcaccgcgg gcgcgtggga
     1921 cgcgtacttc ttctacgccg acggcgagcg ggtcgatgcc caccacgccg cgtgcccgcg
     1981 ggtctcggcg gtgctcggcg cgctgccgct cgaccacgtg cgcgaccacg ggccggaggt
     2041 ctgcttctcg atcctgcggc ccggcgcgca catcctgccc caccgcggcg tcaccaacgc
     2101 gcgcgcagtg ctccacctcg ggctggagat ccccgacggc tgcgcgctcc gcctcccggc
     2161 ggtcgagcag ctcggatggc aggtcggccg gtgcttcgcg ttcgacgaca cctacgagca
     2221 cgaggcgtgg aaccggagcg acaccacgcg cacggtcctg ctcggcgaca tctggaatcc
     2281 gtacctgcgg ccggccgagc gcgaggccct ggccgagctg gtcggcgcga tcggggactt
     2341 caaccgcgcc gccacgccca ccgcgcgcgc ctgagcagcg agacagcagc ccgaaacaca
     2401 aacgagccgg cggggccggc tcgttcgaga gctacgcgcg tcgtgcgtcg cgtgcttgcg
     2461 ctgacctaga acagctggct gaggcggatg tagaactgcc ggccgaggaa gtcataggcc
     2521 gactcgtccg cgttcagcac gggaccgttg tagatgatgg gcggggagac gttcacgacg
     2581 ttgttcatgc cggccgtgat gcccgtcgtg ccctggctgg tcttcaggtt gtagctgacg
     2641 aacacgtcgc ccgtggcgta cttcgagacc tcgcgccgca gggcggtcgg gtcgttgcag
     2701 ctgtcctgct cgcactcctt gaagctgtcg atgaaccgga agttgaagcc ggcgccaaca
     2761 cccgacgggt ggacccacgt cgtgaagatg ttgaacttca ggtccgggaa cacgccgagg
     2821 tcgtagaacc cacgaccatg gaggatgttc tccttcatgg tgtccgggtc cttggtgccg
     2881 gtgtcgacgt tgtactggaa caggtacgtg ccttcgaatg cgtggcggaa cgtaccgaac
     2941 tggttccggt actgatacgc cgccgagaag tcgaggcccg acgtggtcac gccaccgacg
     3001 ttctggatca ggtcgaagat gtggctgatg ccgtgggtga ccggatcgcg cgtgatctgg
     3061 ttgcagaact tggggtcccc ggcctggtag cactgggtca ggatggtctg cacaggcagc
     3121 gtggtgatcg cgtcggtgat gtcgacgtgc cagtagtcga gcgtgaagtc gaggccgcgc
     3181 gccggctcgt acacgaggcc ggccgtgccg accgtgccct tctcgggctg gagcgccggg
     3241 ttaccgccga acttcgcgcg ctgctgcgac gtgccgaact gcgagcccgt cggcacacca
     3301 ccgccgctcg tgttcgggtt cgcgcactgc gcggcgacat tcgccggcag catgatcggt
     3361 gccgtcttgc ccggcggctt ggcgtcgcac gggtccgcga ggttcgggaa gctgtcggcc
     3421 tgacccgaga acagctcggc gacgttcggc gcgcggaacg cgctgccgta ggtaccgcgg
     3481 acggcgacgc cgcccagcgt gcggacgagg ccgctgacct tgccggtcgc gcccgaaccg
     3541 aacgtgttgt agtcgtacgc gcggccggcc gcgttgatct cgacccactt cagggtctcg
     3601 ccgcccgaga tcggaacgac cgagagctca ccgaagccct cgaacgtgtg gtacgagccc
     3661 agggtcggct cgaacgcgtt gccggtggtg tcgccggtcg aggtcagcgg gtcgggctgc
     3721 tggccaccgc gctcgaagcg atagtcaccg ccgatcgcga gcgagatgtc gccgtggttg
     3781 ggcaggtcga gcagcttgcc gctcgccgtg agcagggcgg tgtgctgctc gttgaagccg
     3841 gactgcgtgc cggtgaacgt caggtagttg atcgcctccg gcgtgatgtt ggcttgcgag
     3901 atcgggcccg agtgcagcag gtcgagcggg acgcagccgt cgatcacgtc ggcggacgac
     3961 gtaccgcagt gcgggccctg ggcgtccttg aagctcggac ccagcgcgtt gcccaggtgg
     4021 ctgaggatca ggtcgccgtg ggtcgcctgg gtcgagtcgt tgcggccgta gttgtacgag
     4081 ccctcccact tccagttctt gagcgccggc acctgctcgt cgatcgagcc gttgaggccg
     4141 accaccaggc gggtcgtgtt gacctcctgc ttgaaggtac gcgggccgaa ctcgacgagg
     4201 cggcggttgt agtcgacgac gtcctggccg aacgggttgt agatgctgtc ggccgagacc
     4261 ggcgtgccga acagcgcgag ctggaccggc tcctcggcga gctgctgctc cgacttgcgg
     4321 ctgttgaact ggccctcgaa gaacacgtgg gtgttcttgg tgatgtcgta gtggccgttc
     4381 gagaacaggt tgacgcggct cgacggcgtc agcaggtagt tcagcggctg gaagttgtac
     4441 tggtcaccga ggttggtcgc ggtggggctg atgaagttgc ggaagccgcc gttgccgtcg
     4501 gccgtgcagt acttcgtgtt gcagccgacg atgttcagcg gcgggccgcc gttgggcatc
     4561 gtgttgatgc ggccggtcgg cgacgacgac gacccgagca gcgtggcgat gatgcagtcg
     4621 cccttggact gcatgtcggg cgtgcaggag tagtcgtacg cgtaggtcgc cttggagaag
     4681 tcacggtcgc cggccatgac cggcttctgg ttctggtagc cgccggagaa cgtgatgttg
     4741 ccgcggttcg acgagtggcc ggtcacgaag ctcagatcgt agttcgtgcc gtcggtgtgg
     4801 ttcgtcgtcg cggtgtagac gttggcctcg gtgccgttca tgttggtccg cgtgatcacg
     4861 ttgaccacgc cggcgatcgc gtccgatccg tagatcgccg atgcgccgtc cttgaggacc
     4921 tcgacgcgct cgatcatcgc cagcgggatc gtcccgaagt cgaccgagct gtcggcgccc
     4981 agaccgctcg atacgacgcg gcggccgttg agcagcacca gggtgcggtt ggtgccgagg
     5041 ctgcgcaggt tgatgcgcgt cgagccgtcg ccaccgttgt tgttctgcgc gttgatcgcg
     5101 ttgccctgcg cggggatctt ctgcagcacg tcaccgacgt tggtgatgcc ggcgctctgc
     5161 agcttctcct tgtcgaccac cgagaccggc gacggcgaat cgacttcctt gcggccgatc
     5221 agcgagccgg tgacggtgat gacctcggtc ttggcttcct gctcggcgag ctttgcgagc
     5281 tcctcgtcgc tcaacgccgg ggcctgtccg gccggggcct ggccggccgg ggcctggccg
     5341 gcctcggggg ccggagcagg agtgggttgt ggttgcgcag gggccggctg tccagacgca
     5401 tcagccggct gcggtggatc accgctgggc tgcgcctgat cagctggttg cgcttgcgcg
     5461 atccgcttcg cccagcgcat gggcgaccat tgcgaaccgg gcgccttagt cggccgcatg
     5521 cgcagcgact tcggcgcgtc attgtcggct gcgacaggcg caacaccgac gaggatcaaa
     5581 cagacactgc aagctagagt cttcattcgt gtcattcgcc tgctcccagt tgcagccagc
     5641 ctctcacact gccgacacgt tctgcctggt agtcaaccct aacttgtgat gacgtggggc
     5701 aactcgaatg aggttcatgg cgtcccaatt acctcaatca attgccgcac cccactgaac
     5761 taacgttccc tgaattaccg cctgggtagc atattttgca attgatgtaa attcccggcc
     5821 caaggcctca atttacattc cagacgaaaa cgccagccgc cctgacagaa aaatccggtc
     5881 gcgactggcg atgatggtgt tggacgaaga aacgcggtcc tattgctcct gctccttgtc
     5941 ctggtttcgc agggtgagga cttcttcgcg gacccgttgc gcgaacgcct tgagctcctg
     6001 catcgagttg cgaacacgcg tgcccgcggc cttgttgccg tcccggtaga acttctcgaa
     6061 gtccgtcgcc atgccggtga cgagccgctg gagctctgcg aagcggttgg tcggagccgt
     6121 ggtctcggac tcggtggtct gggtgtcgct gttggtctcc atgagggttg acaccttgcc
     6181 ccgagagtct gacatcggca aaaatgcgtg gatatgtgga aaactgttgg ccttgcggca
     6241 ccggccctcc gcggagcagc ctcgacctga ggcacccggg gtcgttacaa tcggggtatg
     6301 cgaactacag cagccgccat cgcagccctc ctgatgtgcg gggcatgcgg gtcgtccaag
     6361 ccgccggagg gccccaggcc ggacgatcgg atcaaggcgc tggaggccga gctggccaag
     6421 accaaggacg atctggagaa ggccaacgcc gcggccgacg agctgcgcaa gaccgaggcc
     6481 gatctcaagg ccaaggccga cgaggcgacc gcgctgcagg ccaagctcgc cgaggcggtc
     6541 ggtcagagcg gcgaggtgac cactgccgac ggtgcggtga agctcgagct ggtcgatcag
     6601 atcctgtttc cgaccggcga ggccgagctc acgccgaacg gcaaggacgt gctgtccaag
     6661 gtcggcgccg cgctcaagga gatcggtgac aagcagatct gggtccaggg ccacaccgac
     6721 gatcaaccga tctatccccg caagcccaag ccggcggagc cgccgcctgc cgccaaggtc
     6781 gccaagggca agaaggacaa ggcggccaag cccgatccca gggccagcaa gcccgatccc
     6841 aaggccaagc cggacaccaa gcccgacacc aagcccgaca ccaatgatgc tgtgctcgcg
     6901 ttcgtcacca actgggagct gtcggcggcg cgcgcgctca ccgtcgtcca ctacctgcag
     6961 gacgacgcga agatcgatcc gacccggctc gcggcggtcg cgtttggcca gtaccggccg
     7021 atcagcaaga ccaaggccaa gaaccgccgg atcgagatcg tgctgtaccc gcgcgccaag
     7081 ctgacgccca agtgacgccg gggcgtcatg ccccgtcggt cgcgatgaca agcgcagaaa
     7141 tatggccgca acaaacggcc gcatgcgatg tgatacgcga tcggccatga gccgaacctt
     7201 gcgggtggtg atcgcgatcg gagtcgttgc gatcgcgggc gccgggtggt gggtgcacgc
     7261 ccgccgcgtc caggcgcccg ccgcgaccga ggcatcggca ccgagcgcgc ccggcgcggt
     7321 ccgccgcgat cggccgcagc gcggggaccc cgccgagttt ccccgcatgg tgatcgatga
     7381 cgacccccgg ggcacgctgc gcctcgaggg tcaggtgatc gacgccgagg accacggggt
     7441 cggcggcgcg accgtggtga tcac
//
LOCUS       DASVOR010001935         4865 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_546584,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001935 DASVOR010000000
VERSION     DASVOR010001935.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546584"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..328)
                     /locus_tag="VF516_35910"
     CDS             complement(68..328)
                     /locus_tag="VF516_35910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113189.1"
                     /translation="MFTTQPLTIDTSVLSQVIGGASGPDIQGQCGTPPPNGSSNSWGF
                     HASLPLSKIPYVGRYLPDINIGGNGQRTSDYKTCVDAVTGRK"
     gene            1072..1782
                     /locus_tag="VF516_35915"
     CDS             1072..1782
                     /locus_tag="VF516_35915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113190.1"
                     /translation="MFRLKVPLALYVYHPGQGCLKSDSKQILSEDLDVSVLRTTGDKD
                     DQLVVGIDKDKLQPKDDKVKSKDDKRKERAYFCIDRIDFNHLLNAGSIIKIYDKWHSD
                     MVYGLSLVLPFKLRPATAGFNTALTESLTLGGVLGARWRISRFLDYHVDFPVVTAGLS
                     TLDIEQGRAPSTMDGGKQVLGFTASVGAVLELTDFQLGLMVGVDHAPGDQGQGWVYND
                     KVWYSFSIGYTFLKNPAK"
     gene            complement(1808..2974)
                     /locus_tag="VF516_35920"
     CDS             complement(1808..2974)
                     /locus_tag="VF516_35920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113191.1"
                     /translation="MLVPVFGLGIGLGLGALAGPAAAAQRVELAGRVTASRTVLSQRR
                     VGEARYIAISDLFYRDRAAVVEVLDLATRSVIQVAAKRALFERRFGVSRGGSVAVPQG
                     DVVVYRDGVVGLALASAPVISPRRYWYAELDARTGKLLRVADLAALRDGEQLEVIGAD
                     AGGGAAWFAITRLGTHGRSLVLRRLDLGSLEQQDAQRVALGARAESHGHEHSVRVHAA
                     ADFSRFAIVEYAEDGVGMAPGHVYVVDPVAGTSFAVAAPPAAYGVAFSADGRYIYLGS
                     AQRGTISRIDVAGGRIDKQVAGPRFLHHLVISPSGKKLFALASSSSYAVYDLPDLQAR
                     SDATHPAGLAAAMAELHGNGVASLDGAYFVAPEAEDPRKPHAGDRGYVIARLVE"
     gene            complement(3119..>4865)
                     /locus_tag="VF516_35925"
     CDS             complement(3119..>4865)
                     /locus_tag="VF516_35925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113192.1"
                     /translation="IQLLDKADIAGNARVTVLDNPPPGVNAPPYLVVGQTENPIILRD
                     DGIGADVKAGDHIYSGFTNVDQNALAVRSRNEAALISQFKLQSVPTFAGRAVSGSAAP
                     VAFDINSFSNGVAVAFDPSIIFAPTSPAPGKSLLINDLNVVGAVGDPSRVWDPCTGAG
                     TRMGAFTFGHLMTEMANQPLTGINPSTFVMQWLQTWQASQTINTFNVPARPNIATQII
                     NPWLTASGGVSLDLGIAPFRLLAIVNRLDLRTGSGSYGGSTGNAGELRFVFGAVKPGR
                     TCQLLPFTVILEYGVPIQGCLPVKSWAQQWVNLSSLALGSATYNAALQAITDQVVLRN
                     DAPGKPNGSALNQLRTNERAIGVPWELRQFNLLSPIDFLHEAPVAQTPDVRFNAADPG
                     WSGSTSLDTFITSTPAGATVPLAFLGGPFLGGASPVTGDNLGYFWNTSTLDLDPSHLG
                     NWNQRRHLFSLNTCNACHTGETRTTFTHISPTTPLGSPAALSGFLTGITVADPAFGAP
                     TRTFNDLARRQSDLVGVANAACLAFPVVQPELVKMALDGDPLPEVLVSQPVAPVSEQG
                     TFFVEDFFKTSIPVD"
BASE COUNT          765 a         1748 c         1656 g          696 t
ORIGIN      
        1 cccgccgcgc ccggcacccg cgcgcccgac gaagtagcgc cggctgcgcg aggcgagccc
       61 ggcccgctca cttcctgccg gtcacagcgt cgacgcaggt cttgtagtcg ctggtcctct
      121 ggccgttgcc gccgatgttg atgtcgggga ggtacctgcc gacgtacggg atcttgctga
      181 gcggcaagct ggcgtggaac ccccagctgt tgctgctgcc gttgggcggc ggcgttccgc
      241 actgcccctg gatgtcgggg ccgctggcgc cgccgatgac ctgggacaga acgctggtgt
      301 cgatcgtgag aggctgggtc gtgaacatgg ggcttgctcc tggaaggccg cgtcgagcgg
      361 cgtttttgat gtccagccgc cagtgcagcg cgcatgccgt tcgacccgcc cagcaaaatt
      421 gcgcgctttg cgaaggcggc gcacagatcg cgtcgctcgc ggtcgacgaa ccgccgaccc
      481 gggtgatcgg tggccgacaa gcggatcgga gaatgggccc ggccacgcct ccgcgcagca
      541 ttgtgcgcca tgaacgccgg cgcgcggccg gcccgcgatg acccgcggcg tcaccggcaa
      601 gccatgcgta ccacagccgg tccggcgcac ccgctgccga ggttgcgccc gcgcaccggc
      661 tcatcgagcg tttacgccct ggcacctcga ttgctcgagt cgagctcaac cgggcgtgca
      721 gatccagcac gcgctgccgg cgtgtccggg aggaccagcg caacccggga ggactcgaca
      781 tggggcgtgc gggtgcatcc cacgccccgg gaggctgtca tgagatcgac atggggaagc
      841 ggcaaagcat cacgactggc gtgcatcctg ctcgcaggac tcggcgcgcg gatctcgatc
      901 gcgcaggcca atcaaccagc cgggggcacg ctgggagcgg tcgcgccgac gccttcggag
      961 gctccgccgc agccgcagcc gcctccgccg ccgactccgc tgacgacgag ctcgtcaggc
     1021 gggtttgtca acgaagagga attgatcccg aaaaccggag acgacaaatt catgttccgg
     1081 ttgaaggtcc cgctggccct ctatgtctac catccaggcc agggttgcct gaaaagtgat
     1141 tccaagcaga tcctgagcga agacctcgac gtctccgtgc tgcgaacgac cggggacaag
     1201 gacgaccagc tggtggttgg aatcgacaag gacaagctcc agcccaagga cgacaaggtc
     1261 aagtcgaagg acgacaagcg caaggagagg gcctacttct gcatcgaccg gattgacttc
     1321 aatcacctgt tgaacgccgg ctccatcatc aagatatacg acaagtggca cagcgatatg
     1381 gtgtacgggt tgagcctggt gctcccgttc aagctccggc ctgccaccgc cggcttcaac
     1441 accgcgctca ccgagagcct cacgctgggc ggcgtcctcg gcgcgcgatg gcggatcagc
     1501 aggttcctcg actaccacgt cgacttcccg gtggtgaccg ccggcctgag cacgctggac
     1561 atcgagcagg gccgcgcccc ctccacgatg gacggcggca agcaggtcct cgggttcacc
     1621 gcgagcgtcg gcgccgtcct cgagctgacc gatttccagc tcggcctcat ggtcggggtc
     1681 gatcacgccc ctggtgacca gggtcaaggc tgggtctaca acgacaaggt ctggtactcg
     1741 ttctcgatcg gctacacgtt cctgaagaat ccggcgaagt gagcgggccc caccgcagga
     1801 tccgccctca ctcgaccagg cgagcgatca cgtacccgcg gtcgccggcg tgcggcttgc
     1861 gcgggtcctc ggcctcgggc gcgacgaagt aggcgccgtc gagcgaggcg acgccgttgc
     1921 cgtgcagctc cgccatcgcc gccgccagcc cggccggatg ggtcgcgtcg ctgcgcgcct
     1981 ggaggtccgg caggtcgtac accgcgtagc tgctcgacga cgccagcgcg aacagcttct
     2041 tgccgctcgg cgagatcacc aggtgatgca ggaaccgcgg cccggcgacc tgcttgtcga
     2101 tccgcccacc ggcgacgtcg atccgcgaga tcgtcccgcg ctgcgcgctg cccagataga
     2161 tgtagcggcc gtcggccgag aacgccacgc cgtacgccgc cggcggcgcg gccaccgcga
     2221 acgaggtccc cgccaccgga tcgaccacgt agacatggcc cggcgccatc ccgacgccgt
     2281 cctcggcgta ctcgacgatc gcgaaccgcg agaagtcggc cgcggcgtgg acgcgcaccg
     2341 agtgctcgtg gccgtggctc tccgcgcgcg cgccgagcgc gacccgctgc gcgtcctgct
     2401 gctccagcga gccgaggtcg agccggcgca gcaccagcga gcggccgtgc gtcccgagcc
     2461 gcgtgatcgc gaaccacgcc gcgccgccgc ccgcgtcggc gccgatcacc tccagctgct
     2521 cgccgtcgcg cagcgccgcg aggtccgcga cccgcagcag cttgccggtg cgcgcgtcga
     2581 gctcggcgta ccagtagcgc cgcggcgaga tcaccggggc cgacgccagc gccagcccga
     2641 ccacgccgtc gcggtacacc acgacgtcgc cctgcgggac cgcgacgctg ccgccgcgcg
     2701 acaccccgaa ccggcgctcg aacagcgcgc gcttggccgc gacctggatg acgctccggg
     2761 tcgcgaggtc gagcacctcg accaccgcgg cgcggtcccg gtagaacagg tccgagatcg
     2821 cgatgtagcg cgcctcgccg acccggcgct gcgacagcac cgtgcgcgac gccgtcaccc
     2881 gaccggcgag ctcgacgcgc tgggcggcgg ccgccggccc ggcgagcgcg cccaggccga
     2941 ggccgatgcc gaggccgaac acaggcacga gcacgagccg gctggatcgc atgtgcgatc
     3001 cggatccacg agcgggcttg gcagtcgaca ccggttcgcc accggccagt atgacacgcg
     3061 agccgcctcg gtgctcgccg gccgcggcgg ccccggatcg gtgcgccggc gctcggcctc
     3121 agtcgaccgg gatcgaggtc ttgaagaagt cctccacgaa gaacgtgccc tgctcggaca
     3181 ccggcgccac cggctgcgac accagcacct ccgggagcgg gtccccgtcg agcgccatct
     3241 tcacgagctc gggctggacc accgggaacg ccaggcacgc cgcgttcgcc accccgacca
     3301 ggtccgactg gcgccgcgcc aggtcattga aggtgcgcgt cggcgcaccg aacgccggat
     3361 cggcgaccgt gatgccggtc aagaacccgg acagcgccgc cggcgagccc agcggcgtgg
     3421 tcgggctgat gtgcgtgaac gtcgtgcggg tctcccccgt gtggcacgcg ttgcacgtgt
     3481 tgaggctgaa caggtggcgc cgctggttcc agttgcccag gtgcgacgga tccaggtcga
     3541 gcgtgctcgt gttccagaag tagccgaggt tgtccccggt gaccggggac gcgccgccga
     3601 ggaacggccc ccccaggaac gccagcggca ccgtcgcccc ggccggcgtg ctcgtgatga
     3661 acgtgtcgag cgaggtcgag ccgctccacc ccgggtcggc ggcgttgaac cgcacgtccg
     3721 gcgtctgggc caccggcgcc tcgtgcagga agtcgatcgg cgacagcagg ttgaactggc
     3781 gcagctccca cggaacgccg atcgcgcgct cgttggtccg cagctggttg agcgcgctgc
     3841 cgttgggctt gcccggcgca tcgttgcgca gcacgacctg atcggtgatc gcctgcagcg
     3901 cggcgttgta cgtggccgac ccgagcgcga gcgagctcag gttgacccac tgctgcgccc
     3961 agctcttcac cggcaggcag ccctggatcg gcaccccgta ctccaggatc acggtgaacg
     4021 gcagcagctg gcacgtccgg cccggcttga ccgcgccgaa cacgaaccgc agctcgccgg
     4081 cgttgccggt cgatccgcca tagctccccg agccggtccg cagatcgagc cggttcacga
     4141 tcgccagcag ccggaacggc gcgatcccca gatcgaggct gacaccgccg ctcgccgtca
     4201 gccacggatt gatgatctgc gtcgcgatgt tcggccgcgc cggcacgttg aacgtgttga
     4261 tcgtctggct cgcctgccag gtctgcagcc actgcatcac gaacgtcgac gggttgatcc
     4321 cggtcagcgg ctggttggcc atctcggtca tcaggtgacc gaacgtgaac gcccccatcc
     4381 gggtcccggc gccggtgcac ggatcccaga cccggctcgg atcgccgacg gcgccgacca
     4441 cgttcaggtc gttgatcagc agcgacttgc ccggcgccgg cgacgtcgga gcgaagatga
     4501 tcgacggatc gaacgcgacc gcgaccccat tgctgaagct gttgatgtcg aacgccaccg
     4561 gcgccgcgct gcccgacacc gcccggccgg cgaacgtcgg cacgctctgg agcttgaact
     4621 ggctgatcag cgccgcctcg ttgcggctgc gcaccgcgag cgcattctga tcgacattgg
     4681 tgaaccccga gtagatgtgg tcgccggcct tgacgtccgc cccgatccca tcatcgcgca
     4741 ggatgatcgg attctcggtc tggccgacca ccagatacgg cggcgcgttg accccgggcg
     4801 gcggattgtc gagcaccgtc acccgcgcgt tgcccgcgat gtccgccttg tcgagcagct
     4861 ggatc
//
LOCUS       DASVOR010001936         5745 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341276,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001936 DASVOR010000000
VERSION     DASVOR010001936.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341276"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VF516_35930"
     CDS             <1..455
                     /locus_tag="VF516_35930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8113193.1"
                     /translation="RARWGTPPPAGIGRGLAIHESFGSVVAQVADVSVADGRIRVHRV
                     VCAIDCGLAVNPAGITAQMESSILYGLSAALYGAIHIDHGRVRESNFHDYPVLRIHEA
                     PAIEVVIVPSKAAMGGGGEPGTPPIAPAVANAVFALTGKRLRALPFQV"
     gene            508..1494
                     /locus_tag="VF516_35935"
     CDS             508..1494
                     /locus_tag="VF516_35935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HEX8113194.1"
                     /translation="MAATLHALLSLSGIALLYWGAEWLIRGSAQVARAFGVKPLVVGL
                     TVVAYGASAPELTIATEAALTHHEPIALGVVIGSCAANISLILGITALISPPTIDGRI
                     IRREVPVLLGSAIAVPLLFRNGILSRLEGAVLVICAVVFTIATLVVSSRLEHDDPLEQ
                     EARRTEENAATLGGKGRPQGSRGAAALQTAIGLILLVIGADLFLRGGRDLGKDLGMSE
                     RMLGLTVIALGTALPELIGSIVAASRGNAALAIGSVVGSNVLNVFLVLGVTAYLRPIH
                     AGEVLHLSDLLGLVGITLLGILMLRGSRKISRGEGLVLVAAYVAFLTTAAMF"
     gene            1591..2736
                     /locus_tag="VF516_35940"
     CDS             1591..2736
                     /locus_tag="VF516_35940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113195.1"
                     /translation="MKAVLGVLGMVAACGSGDASEVWTACPDAPGTTSRIAAGVLIGA
                     DATRVFFQGSAPGVMAWPKGGGVATRFTSEELGTNLAISGESYYWTAPSNPAITVTTA
                     RGAIHQIVPLASSLSTVKQLVAAPGGGVYALTTCAGPACVSAPPDTRPLDTIEQVSAD
                     VAASQILVSGPANIRAIAATGDALFWVEVSRPSGQYRIGRRDALTGELSTVVDGLHLK
                     AEPGSLVIAGHELFDVGAGFPPSGLALERRNLADGTLLDAYPVPALPTLWEDMLQFVG
                     TPQCFAIDTDLYWTQPRGQTIEEFMAEVCSVPTGLFRLRLSDRTVTSIDPHASPSPFV
                     IDEDVVYYTYSFLAPFCCNYHGMGCPVPAEVPGPSEVHCFRKPAAAP"
     gene            3314..4249
                     /locus_tag="VF516_35945"
     CDS             3314..4249
                     /locus_tag="VF516_35945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113196.1"
                     /translation="MDATRVFLNSGQWFWPKAGGSAAPFIPRGLGEQLTLNGGTYYWS
                     DLGSRAVNITDASGALVRSIPVANPVNGPAQLLPAGDAGVYVLTRCFSTSDRTCPAST
                     TDTIELIHDAASDAQTVVTPAPSTMIDAIAAAGNVLIWAERNPTDASEQIQQRDATTG
                     AITALVTLPEPLATRSGGLVIAGDAFFDAIDFFSRIERRALADGALLDSYAVEGTSAE
                     WLTYDTDLYYTQQPTFSIGPEGQCPVPLGVSRIRVSDRSMKRIDANALSAPVLIDGDV
                     LYYTDLEISMCCSGNGHECPPSRPAPSHVRCYRLR"
     gene            4763..5665
                     /locus_tag="VF516_35950"
     CDS             4763..5665
                     /locus_tag="VF516_35950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113197.1"
                     /translation="MFLSSGEGFWPKAGGSAAPFITGGAGTHLTLQGGNYYWNDVEHR
                     VVHVTDASGALVRTIPVASPTNSPVELVAAGQAGVYVLTRCVSTQDMICPASATETIE
                     LIRDAASDAQTLVAPAPPTRIDGIAGNGYVVIWAEGSQIQKLDATTGTITPLVQLYGG
                     LSSRAGNLLISGDMFFAVRSGPVIERHALSNGALLQTQDLALAVGYFSVEWLAYDPYH
                     TNLYFAGRYVFGGVAGQGQCDGPPDLYRLGLSDKGPQKIFSVVRPDRMLLEGEALYLT
                     ATDLGGTSTCPAAGPHSFVACYRL"
BASE COUNT          789 a         2039 c         2013 g          904 t
ORIGIN      
        1 agcgcgcgcg gtggggcacg ccgccgccgg cgggcatcgg ccgcggcctg gcgatccacg
       61 aatcgttcgg cagcgtggtc gcccaggtcg ccgacgtgtc ggtcgcggac ggccggatcc
      121 gcgtgcaccg cgtggtgtgc gcgatcgact gcggcctcgc ggtcaacccc gcgggcatca
      181 ccgcacagat ggagtcgagc atcctctacg gcctgtcggc ggcgctgtac ggcgcgatcc
      241 acatcgacca cggccgggtc cgcgagtcga acttccacga ctacccggtg ctgcgcatcc
      301 acgaggcgcc ggcgatcgag gtcgtgatcg tgccgagcaa ggcggcgatg ggcggcggcg
      361 gcgagcccgg caccccgccg atcgcgccgg cggtcgccaa cgcggtgttc gcgctgaccg
      421 gcaagcgcct gcgcgcgctg ccgttccagg tctgaccggc ggccgagtcg tcgccccacc
      481 ggccgcgact cgtgcgacac tttcgcagtg gccgcgacgc tccacgccct gctctcgctg
      541 tccgggatcg cgctgctgta ctggggcgcg gagtggctga tccgcggctc ggcccaggtc
      601 gcgcgcgcgt tcggggtcaa gccgctggtc gtcgggctga ccgtggtcgc ctacggcgcg
      661 tcggcccccg agctgacgat cgcgaccgag gccgcgctga cccaccacga gccgatcgcg
      721 ctcggcgtcg tgatcggctc gtgcgccgcc aacatcagcc tgatcctcgg gatcaccgcg
      781 ctgatctcgc cgccgacgat cgacggccgg atcatccggc gcgaggtccc ggtgctgctc
      841 ggctcggcga tcgcggtgcc gctgttgttc cgcaacggca tcctgtcgcg gctcgagggc
      901 gcggtgctgg tgatctgcgc cgtggtgttc acgatcgcca cgctcgtggt gtcgtcgcgg
      961 ctcgagcatg atgacccgct cgagcaggag gcgcggcgca ccgaggagaa cgccgcgacg
     1021 ctcggcggca agggccggcc gcagggctcg cgcggagccg ccgcgctgca gaccgcgatc
     1081 gggctgatcc tgctggtgat cggcgccgac ctgttcctgc gcggcggccg cgacctcggc
     1141 aaggacctcg ggatgtccga gcggatgctg ggcctgaccg tgatcgcgct cggcaccgcg
     1201 ctgcccgagc tgatcggctc gatcgtcgcc gcgtcgcgcg gcaacgccgc gctggcgatc
     1261 ggctcggtgg tcggctcgaa cgtgctgaac gtgttcctcg tgctcggcgt caccgcgtac
     1321 ctgcggccga tccacgccgg cgaggtgctc cacctgtccg acctgctcgg cctggtcggc
     1381 atcacgctgc tcggcatcct gatgctgcgc ggatcgcgga agatctcgcg cggcgagggg
     1441 ctcgtgctgg tggccgccta cgtcgcgttc ctgaccaccg ccgcgatgtt ctgatccggg
     1501 cgatcgcgca gcgcgtgcgt acggcgcacg gcgctgcgcg cgacgatctg tcggctcgtg
     1561 cgaccgcccg ccgtgattag agtggaacgc atgaaggcgg ttttgggggt actcggtatg
     1621 gtcgccgcat gcggttcggg agacgccagc gaggtctgga cggcatgtcc ggacgctccg
     1681 gggacgacct cgaggatcgc ggccggcgtc ctgatcggcg cggacgcgac gcgcgtgttc
     1741 ttccaggggt cagcgcccgg ggtgatggcg tggcccaagg gcggcggagt cgccacccgg
     1801 ttcacgagcg aggagctggg gacgaacctc gcgatcagcg gcgagagcta ctactggacg
     1861 gcgccgagca accccgcgat caccgtgacc accgcccgag gtgcgatcca ccagatcgtg
     1921 ccgctggcca gctcgctgag cacggtcaag cagctcgtgg ccgcccccgg cggtggcgtc
     1981 tacgcgctga ccacctgcgc cggccccgcg tgcgtgtcgg ccccgcccga cacgcgaccg
     2041 ctcgacacca tcgagcaggt gtccgcggac gtcgcagcct cgcagatcct cgtgtccggc
     2101 ccggcgaaca tccgcgcgat cgcagccacc ggcgacgcgc tgttctgggt ggaggtgtcc
     2161 aggccctcgg ggcagtaccg gatcgggcgg cgcgatgcgc tcaccggaga gctgagcacg
     2221 gtggtcgacg gcctgcatct caaggccgag cccggcagcc tggtgatcgc cgggcacgag
     2281 ctgttcgacg tgggcgccgg tttccccccc tcggggctcg ccctcgagcg ccgcaacctg
     2341 gccgatggga cgttgctcga tgcctacccg gtgccggccc ttcctaccct ctgggaggac
     2401 atgcttcagt tcgtgggaac gccacagtgc ttcgccatcg ataccgacct gtactggacc
     2461 cagccgcgcg gccagacgat cgaggagttc atggcagagg tctgcagcgt tcccacggga
     2521 ctgttccggc tccggctctc ggaccggacc gtgacaagca tcgacccgca tgcatctccg
     2581 tcgccgttcg tcatcgatga agacgtcgtg tactacacgt actcgttcct ggccccattc
     2641 tgctgcaact accacggcat ggggtgcccc gtcccggccg aggtaccggg tccgtccgag
     2701 gtccactgct tccgcaagcc cgccgctgcg ccgtgaccgg cccgggcgcc gcgccccgcc
     2761 ccgaccggcg ccgagcgagg tgcgcggtta ccggctgcag tagcggcgcg cgggtttgcg
     2821 catgcccgat cgcgcctcgc tcccccgcgt agccggctgg ccgatcggac gctcgcgctc
     2881 ggcctcggtt tccccgtggt tcagcgtcgg cgatgggccg aatgcgcccg cgcgaccggt
     2941 acacgcacgg cgtcgcaggg ctgacccagc tgcgctggca agaaccacga cgcgcgtgtc
     3001 gcggttcatc gcgcgcgcgt gaggcgcggc atgcggcgga cagaacgctg catcgacggc
     3061 gcggcccggt gctacggtgc ggctggtcgc tgtcgacgag ctggagcctg ccatgcgatg
     3121 cgcgatgatg ggagtctggt gcgcggtgct gagcgctctg gttgcctgtt ccgggaaaga
     3181 tacaaccggc ccccccggat cgcctcccgc ggatgcggcc gggcccgacg cgttcacgct
     3241 ctcgctgggc aacaccgcgt gccctgcaac ccccggcgtc tccgcgccca tcgcggcggg
     3301 caggctcgcc gggatggacg cgacgcgcgt gttcctgaac tccggacagt ggttctggcc
     3361 caaggccggc ggcagcgcgg cgccgttcat cccccgcggg ctgggcgaac aactcacctt
     3421 gaacggcggt acctactact ggagcgacct cgggagtcgg gcggtcaaca tcaccgatgc
     3481 cagcggtgcg ctcgtccgct cgatcccggt cgcgaatccg gtgaacggtc cggcgcagct
     3541 cctccccgcg ggggacgctg gcgtgtacgt gctcacccgg tgtttctcga ccagcgaccg
     3601 gacctgcccg gcttccacga ccgataccat cgagctcatc cacgatgcgg ccagcgacgc
     3661 gcagaccgtc gtgacgcctg caccttccac gatgatcgac gcgatcgccg ccgcggggaa
     3721 tgtgctgatc tgggcggagc ggaaccccac ggacgcgagc gagcagatcc agcagcgcga
     3781 tgcgacgacc ggcgcgatca cggcgctggt gacgctgccg gagccgctgg ctacgcggtc
     3841 cgggggactc gtgatcgccg gcgacgcgtt cttcgacgcg atcgacttct tctcgcggat
     3901 cgaacgccgc gccctggccg acggcgcgtt gctcgacagc tacgccgtgg agggaaccag
     3961 cgccgagtgg ctgacctacg ataccgatct ctactacacg cagcagccca cattcagcat
     4021 cggccccgag gggcaatgtc ccgtgccact cggagtgtcc cggatccggg tgtcggatcg
     4081 ctcgatgaag cggatcgatg ccaacgcgct ctccgctccc gtgctgatcg acggcgatgt
     4141 cctctactac acggacctgg agatcagcat gtgttgctcc ggtaacggcc acgagtgccc
     4201 gccgtcccga ccggcgccga gccacgtgcg ctgttaccgg ctgcggtagc ggcggcgcgg
     4261 gtgtgcaacg cccgatcgcg ccgcgctcac cgcgcgcgta tccggctcgt cgatcggcgc
     4321 tcgcgctcgg cctgcggttc gccgtggttc agcgtcggcg gcgggccgca tgcgcccgcg
     4381 cgaccggtac acgcacggcg tcgcagggct gacccagctg cgctggcaac caccacgacg
     4441 cgcgtgtcgc ggtttcgtcg cgcgcgcgtg aggcgcagca tgcgcgaggc ggaaacgatg
     4501 caccggcggc gcggcacggt gatacggtgc aggtggtcgt tgtcgacgat ccaggaggct
     4561 gaccatgcga tgcgcgatga tgggagtctg gtgcgcggta tcgagcattc tcgttggctg
     4621 ttccgcaaaa ggggcgtctc caccggatgg acctccgccc gacgcgttcc cgctgtcgct
     4681 gggagatacc atgtgccctg cgactcccct gaccgcttcg tggatcgcgt cgggcaccct
     4741 cgcggggatg gacgggacgc gggtgttcct gagctccgga gagggcttct ggcccaaggc
     4801 cggtgggagt gcggcaccgt tcatcaccgg cggagcgggt actcacctga cgttgcaggg
     4861 gggcaactac tactggaacg acgtcgagca ccgggtggtc cacgtcaccg atgccagcgg
     4921 tgccctcgtc cgtaccatcc cggtcgccag cccgacgaac agcccggtgg agctcgtcgc
     4981 agccgggcag gccggcgtct acgtgctcac ccgctgtgtc agtacccagg acatgatctg
     5041 cccagcctcc gcgaccgaga cgatcgagct catccgggat gcagcgagcg acgcgcaaac
     5101 cctggtggcg cctgcacctc ccacccggat cgacgggatc gccgggaacg gatatgtggt
     5161 gatctgggcg gaaggctcgc agatccagaa gctcgatgcg accaccggca cgatcacacc
     5221 gctggtgcag ctgtacggtg ggctgtcctc gcgggccggt aacctgctga tcagcggcga
     5281 catgttcttc gccgtgagaa gcggcccggt catcgagcgc cacgccctgt ccaacggcgc
     5341 gttgctccag acacaagacc tcgcgctcgc cgtggggtac ttcagcgtcg agtggctggc
     5401 ctacgacccc taccacacca atctttactt tgcagggcgg tatgtgttcg ggggtgttgc
     5461 gggccaaggc cagtgtgatg ggccgcccga cctgtaccgg ctcgggctct cggataaggg
     5521 cccccagaag atcttttcgg tcgtgcgccc cgatcgcatg ctgctggaag gcgaggccct
     5581 gtacctcacc gcaaccgatc tcggaggcac atccacctgc ccggcagccg gaccgcacag
     5641 cttcgttgct tgctaccggc tgtagcgacc ggcgtgcggt tcgcgcgccc gatcgcgcct
     5701 cctcaccgcg cggccgcggt gcgcgccgcc gcctcggcga tgatc
//
LOCUS       DASVOR010001937         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341280,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001937 DASVOR010000000
VERSION     DASVOR010001937.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341280"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..856)
                     /gene="arsN2"
                     /locus_tag="VF516_35955"
     CDS             complement(266..856)
                     /gene="arsN2"
                     /locus_tag="VF516_35955"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HEX8113198.1"
                     /translation="MIGPLVEVPALIPDPASQDPSIPRDEMLRRFRAARDTIRGMLER
                     HAAENGIPVRSPEPARRDDLDAVIALVRASALPTAGIADGFPDGYAVVRDGTAIVAVA
                     GLEVHGDVGLLRSVAVAASRRAGGLGRLLVEDRLRAANAHKLRAVYLLTTTAADYFRR
                     FGFEDARRELAPASLQSSSEFASVCPASATCLAKPL"
     gene            855..1157
                     /locus_tag="VF516_35960"
     CDS             855..1157
                     /locus_tag="VF516_35960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113199.1"
                     /translation="MPAVIYRLTGMVPVVPYGGIGPRLLLARSTVRDNGAPAIMETRE
                     QSTRIGVGVPLGAELPLGPGRAIAELLVQYGTLNHVATGDASTGAVSLAVGYRMMF"
     gene            1230..1439
                     /locus_tag="VF516_35965"
     CDS             1230..1439
                     /locus_tag="VF516_35965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113200.1"
                     /translation="MSDRNADDVRTLVREHYGNLARGIDAGCAPGCADASIDVILSNC
                     VIALDARGKVVEFTPRVPSQPCCPK"
     gene            complement(1501..>1856)
                     /locus_tag="VF516_35970"
     CDS             complement(1501..>1856)
                     /locus_tag="VF516_35970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113201.1"
                     /translation="VEAGQVTAGGQLASFDDSPKDMAANQAGYGVCAANQQLFSFGGL
                     NASPSSGALSASLVNPPPALAGSSWNNEGLTMTHGRYLPGSAVQSSFIFLLGGQTDEP
                     SAASKTTELVIHSVR"
BASE COUNT          278 a          656 c          664 g          258 t
ORIGIN      
        1 ggtcatcgcg gtggttcctc ctgcagattc gctcgcgcag cgcgcgccgg cctagcgttc
       61 cggcagccgg aacggccgtc aagcgaaaac cggacgcaga ccgcggctcg gcggctcgcc
      121 gggaacggat cgttgaccac gagcaccgcg ccttgactgg gagtgcacac gaacgtgccc
      181 gcgcggcccg cgaaaatttg tgcacttccg cgcaccgccc ggtggggctc gtcccccgtg
      241 ggaggacgtg gcgaaccggc aacccttaca gcggcttcgc caggcacgtc gccgaggcgg
      301 gacacaccga cgcgaactcg gacgagctct gcaggctcgc aggcgcaagc tcccgtcgcg
      361 catcttcgaa tccgaaccgc cggaaatagt ccgccgcggt cgtcgtcagc aggtacacgg
      421 cccggagctt gtgcgcgttc gccgcgcgga gccgatcctc caccagcagc ctgccgagcc
      481 cgccggcacg ccgggaggct gcgaccgcga ccgagcgaag caaccccacg tcgccatgga
      541 cctccagacc ggcgaccgcg acgatcgcgg tgccgtcgcg caccaccgcg tagccatcgg
      601 ggaacccatc cgcgatgccg gcggtcggca gcgccgacgc ccgcacgagg gcgatcaccg
      661 cgtcgagatc gtcgcgtcgc gcaggctccg gggagcggac ggggatgccg ttctcggcgg
      721 cgtggcgttc gagcattccg cggatggtat cgcgcgccgc tcgaaagcgc cggagcatct
      781 cgtcgcgggg gatcgaagga tcctggctgg ccggatccgg gatcagcgcc ggcacctcga
      841 cgagcgggcc gatcatgccg gcggtgatct accggctgac cgggatggtg ccggtggtgc
      901 cgtacggcgg catcggcccg cggctgttgc tggcgcgcag cacggtgcgc gacaacggcg
      961 caccggcgat catggagacc cgcgagcagt cgacgcggat cggcgtcggt gtcccgctcg
     1021 gcgccgaact gccgctcgga ccggggcgcg cgatcgccga gctgctggtg cagtacggca
     1081 cgctgaatca cgtcgccacc ggtgacgcca gcaccggcgc ggtcagcctc gccgtgggct
     1141 accggatgat gttctagggc gttgcagcgg ccggtcgatt tcttgcgtcc tcggggcggc
     1201 aagcccgtcc atggaccagg aggctggaca tgtctgaccg gaacgcggat gacgtgcgga
     1261 ccctggttcg cgagcactac ggaaacctcg cgcggggcat cgacgcggga tgcgcgccgg
     1321 gctgcgccga cgcctcgatc gatgtcatcc tctcgaactg cgtgatcgcg ctcgacgcgc
     1381 gcggcaaggt cgtcgagttc acgccgcgcg tgccgagcca gccgtgctgc ccgaagtgac
     1441 ctgcgaggca cccatgcaag ctcccggcgg aatcgcacgc aagctctcgg tcctcgaccg
     1501 ctacctgacg ctgtggatca ccagctcggt ggtcttgctg gcagcgctcg gctcgtcggt
     1561 ctggccgccg agcaggaaga tgaacgagct ctgcacggcg ctgcccggca ggtagcggcc
     1621 gtgggtcatc gtcaggccct cgttgttcca cgacgacccg gcgagcgccg gcggcggatt
     1681 gaccagcgac gccgacagcg cgccgctcga cggcgaggcg ttgagcccgc cgaacgagaa
     1741 cagctgctgg ttggccgcgc acacgccgta gcccgcctgg ttggcggcca tgtccttggg
     1801 gctgtcgtcg aacgacgcga gctggccgcc cgcggtgacc tggccggcct cgacct
//
LOCUS       DASVOR010001938         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341314,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001938 DASVOR010000000
VERSION     DASVOR010001938.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341314"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..312)
                     /locus_tag="VF516_35975"
     CDS             complement(<1..312)
                     /locus_tag="VF516_35975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113202.1"
                     /translation="MTDRDDELVFRGGTILTIDPQYRVLAGDVACRGGAIVQVGGDYT
                     PATRDYVIVDCAGCVVMPGLVQAHVHTCQTLARGRADDLELLDWLRTVIWPYEAALDE
                     AA"
     gene            complement(309..1199)
                     /locus_tag="VF516_35980"
     CDS             complement(309..1199)
                     /locus_tag="VF516_35980"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEX8113203.1"
                     /translation="MTDPELYERIQAAAAAVRARASLTPDRAPQVGLILGSGLGGFAD
                     RLAGATAIDYADIAHFPRSHVAGHAGRLVIGERAGARCVAMQGRVHLYEGHSAQAVAF
                     PARVLIALGARVLIITNAAGGVNPAWPAGTLMLIRDHLDLLRDQALRGPNDDRLGPRF
                     PDMTAAYAPELRELVRGAAAGAGIALAEGVYAAMPGPTYETPAEIRMLQVLGADAVGM
                     STVPEVCVARHMGARVIGISCITNQAAGITGQPLSHDEVTATAARVRTTFEALLDAVL
                     AALVAHRELIPDGFSGKQLS"
     gene            complement(1196..>1533)
                     /locus_tag="VF516_35985"
     CDS             complement(1196..>1533)
                     /locus_tag="VF516_35985"
                     /inference="COORDINATES: protein motif:HMM:NF019448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113204.1"
                     /translation="RARSRDELTLPAGRITAIDSEALGTAAVMLGAGRRTTDDAIDPA
                     AGLVIDAYVGELIEPGAPPQVILCHDLSPGDGRRAEARALIQGAFTIDAPSGPPPAPP
                     GSRILEILR"
BASE COUNT          196 a          550 c          596 g          191 t
ORIGIN      
        1 cgcggcctcg tcgagcgcgg cctcgtacgg ccagatcacg gtgcgcagcc agtcgagcag
       61 ctcgaggtcg tcggcccggc cgcgcgccag ggtctggcag gtgtggacgt gggcctggac
      121 caggccgggc atcaccacgc agccggcgca gtcgacgatc acgtagtcgc gcgtcgccgg
      181 cgtgtagtcg ccgccgacct ggacgatcgc gccgccccgg caggcgacat cgccggcgag
      241 cacgcggtac tgtggatcga tggtcaggat cgtcccgccg cggaacacca gctcgtcatc
      301 gcggtcggtc atgacagctg tttacccgag aatccgtcgg ggatcagctc gcggtgagcg
      361 accagcgcgg cgagcaccgc gtcgagcagc gcctcgaacg tcgtgcgcac gcgggccgcg
      421 gtcgcggtga cctcgtcgtg cgacagcggc tggccggtga tcccggcggc ctggttggtg
      481 atgcacgaga tgccgatcac gcgcgcgccc atgtgccgcg cgacgcagac ctccggcacc
      541 gtcgacatcc cgaccgcgtc cgcgccgagc acctgcagca tccggatctc ggccggggtc
      601 tcgtaggtcg gcccgggcat cgcggcgtac acgccctcgg cgagcgcgat cccggcgccg
      661 gccgccgcac cgcggacgag ctcgcggagc tcgggagcat aggccgcggt catgtcgggg
      721 aaccgggggc cgagccggtc gtcgttgggg ccgcgcagtg cctggtcgcg cagcagatcg
      781 aggtgatcgc ggatcagcat cagcgtgccg gccggccacg ccgggttcac cccgccggcg
      841 gcgttggtga tgatcaggac ccgggcgccg agcgcgatca gcacgcgggc cgggaacgcc
      901 accgcctggg cgctgtggcc ctcgtacagg tggacgcggc cctgcatcgc gacgcagcgt
      961 gcgccggcgc gctcgccgat caccagccgg cccgcgtgcc ccgcgacgtg cgaccggggg
     1021 aagtgcgcga tgtcggcgta gtcgatcgcc gtggcgccgg cgagccggtc ggcgaacccg
     1081 ccgagccccg agccgaggat cagcccgacc tggggcgctc ggtcgggcgt gagcgaggcg
     1141 cgggcgcgca ccgcggcggc ggcggcctgg atgcgctcgt agagctctgg atccgtcatc
     1201 gcaggatctc caggatacgc gagccgggcg gcgcgggcgg cggtccagaa ggcgcgtcga
     1261 tcgtgaatgc tccctggatg agcgcgcgcg cctcggcgcg ccggccgtcg ccgggcgaca
     1321 ggtcgtggca caggatgacc tggggcggtg cgccgggctc gatcagctcg ccgacgtagg
     1381 cgtcgatcac cagaccggcg gccgggtcga tcgcgtcgtc ggtggtgcgc cggccggcgc
     1441 cgagcatcac cgccgcggtg ccgagcgcct cgctgtcgat cgccgtgatc cgccccgcgg
     1501 gcagggtcag ctcgtcgcgc gaccgcgcgc gcg
//
LOCUS       DASVOR010001939         6199 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_49960,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001939 DASVOR010000000
VERSION     DASVOR010001939.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6199
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49960"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VF516_35990"
     CDS             <1..872
                     /locus_tag="VF516_35990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113205.1"
                     /translation="ATATSATPPRSPTAWSRADRQRTCSAHRRAAAARGGGAVQRSPE
                     KPTKEQTKNFCSGGDWLHCGKAASARPQLVVGVRRSDKDNAPHVAQPGLSDGKGHIVV
                     ETPTGVTGGPYVEFYLIRRDGTPIRGGRGLVYVFSKEKEQEVEIDRSAADQVGSDTRA
                     IIVEDRIDEFPKTRDPGHPVRVTEVARRGKWVDARIDLGTAEGASGIYKARLKFNGRD
                     GAPDVEYDARKLTGQDGDSYADFEIDNQDAGQRILNLQDSVKRNVAFATLIVESDDKS
                     KGKGKDKGKDSGK"
     gene            complement(1010..2821)
                     /locus_tag="VF516_35995"
     CDS             complement(1010..2821)
                     /locus_tag="VF516_35995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113206.1"
                     /translation="MTRTVPRLLALALVLAATAPPAWAKDKDGDDVKIDDDDASGTSK
                     KASKKASDGDDPGGSGLQKQDLSGHDLGTSKKDNQFEHDRFFVDKVDSDKTAKGTLVQ
                     GSLTSTTFGYYESGGTIAPAPAGVPSASQFSREYTDLRLQTDFRHIGASRWDARVDVR
                     GRLVNTPAPVTASTDGFTPGSATNVQSGFLGQNELEIKELWLVRNGTRSDLFFGRQFV
                     PDLGGVKIDGLRVDYASSTKFTLLGFGGLYPIRGSRSITTDYQPLKSNDQNDAGRFTG
                     AGGFGAAYRTLDAYGSFGGVVIAPLSAESPRVFGTSSGYWRYGTKLDFYHFVVLDLLG
                     SNAVNAGLTNLSAGLNYKPDPRLRGTLSFNRVDTETLNVQAQAFLSSPDPKVNVVQNE
                     AYLQRIATNEGRGSLSAGLGELQRFEVTAAVAYRYRGDVTLSAPPQMTGAPATIALPA
                     AQSVEVYGAITDRRSFKDLRLGIDGSQIFGVGSATYQRTSSRSLRASAARELADGRGE
                     WEAEVAYSSTKDDSAGKACPPGSDLSTCFGAANSSILSVGGNLYYRFNRDWFAMGSVF
                     LNRTAVTHIEMMGSTTVSTADPAVIGLTGFLRIAYRF"
     gene            complement(2818..5286)
                     /locus_tag="VF516_36000"
     CDS             complement(2818..5286)
                     /locus_tag="VF516_36000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113207.1"
                     /translation="MTRVVGRALALAALLLLLALVRTARADQFFGSSPGPLTRSHGQL
                     DAQDHCNDCHVGDSKELSNDKCLGCHDHQNLGARIAAGKGFHASSMVKGKKCESCHHE
                     HKSRTYDLMGWPSVKGGQKGFDHELTGWQLKGKHAATDCNDCHKARSKEQGLQVFMGT
                     DRGCGSCHAKDQPHKIVRKDLLACERCHGESVWKPAKSATAMQFNHDDRKDAAMPLLG
                     RHKDVACTKCHAKNVYKLPFPRPDSCGNSGCHQSPHGGHLFGARPCEWCHSPTFKDLA
                     NIQVFDHSERTRFDLGPAHKKIKCEQCHTKALGEVKPSRSCEQCHAKDNKHGDRFKEF
                     GSPPRCETCHPSGGPKFTPSNFNHAAHTKFKLEFKHAEVTCRACHRGSSPSDFERFAF
                     NPNKDCKSCHAHAKVHADDEHPDGKYKSAQCLQCHRNGGNINVDTKRSSIIAEVHGPT
                     GSFPLVKRHKDVACASCHTGRDKKGKTSFSDLKPNCNAAGQCHEDSLHKGTLGDKCMA
                     CHSSGVWDALKFDHDQPFPKDAKGQVTAFPLKGEHRKNKCESCHPQRKFAQTPTTCAA
                     EGCHKEDDAHRGRLGDKCEQCHVETGDNVFNHNTMSAFKLDGKHLTVRCSDCHPSVTF
                     KPRPTDCFGCHPEPAVHKGQYGTGCAQCHTTRTWGDVKPLHDVGDFSLRGMHDNIACE
                     RCHRDNRPLAGSGNLCINCHRQDDIHNNSLSPRCGECHTQWSFAPARFDHAKAGCNLT
                     GLHRTIACFDCHKNGNFAALSPQCVGCHHDDALRTTVDTTGARVAMGAINSHFTTTTC
                     ANCHSANTWLGAPGFALGRESVCR"
     gene            complement(5297..>6199)
                     /locus_tag="VF516_36005"
     CDS             complement(5297..>6199)
                     /locus_tag="VF516_36005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113208.1"
                     /translation="KVKRGRYAPLVIWVLGIFAFLLALGEVLLRLYAPTMSYQFATLR
                     ASPTYEGIADSGVLDNVRVRAGEPLAIWCGVIGTALMIIAAIYPIFRRIKLFRWMASN
                     TMWFDFHLMAGTVGPMFVVLHSALRLDTWVSAAFWSMAIVVISGFLGRYLYTQVPALS
                     SGVELEELDHERAFQKARPRLTVAMAEIDRELAIHRARAQEVAMSPSVTRALWWLIFQ
                     DIGRIPATIARRGRLAQLGVDRRVRRDLARRAARMIVIARRQVVAPKAQLLLHSWKKV
                     HVPFTVILTGFSAVHIWVSWSRAW"
BASE COUNT          913 a         2017 c         2260 g         1009 t
ORIGIN      
        1 cggcgacagc tacgagcgcc acgccgccgc ggtcgccgac cgcgtggtcg cgggccgatc
       61 ggcagcggac ctgttcggcc caccggcggg cggcggccgc gcgcggtggc ggcgcggtcc
      121 agcgctcgcc ggagaagccg accaaggagc agaccaagaa cttctgctcg ggcggcgact
      181 ggctgcactg cggcaaggca gcgagcgcgc ggccgcagct ggtcgttggg gtccggcgca
      241 gcgacaagga caacgcgccg cacgtggcgc agcccgggct gtccgatggc aagggccaca
      301 tcgtggtcga gacgccgacc ggcgtgaccg gcggcccgta cgtcgagttc tatctgatcc
      361 gccgcgacgg cacgccgatc cgcggtggcc gcggcctggt ctatgtcttc agcaaggaga
      421 aggagcaaga ggtcgagatc gatcgcagcg cggccgacca ggtcggctct gacacccgcg
      481 cgatcatcgt cgaggaccgg atcgacgagt ttcccaagac cagggatccg ggccatccgg
      541 tacgggtgac cgaggtcgcg cggagaggca agtgggtcga tgcgcgcatc gatctcggca
      601 ccgccgaggg cgcgagcggg atctacaagg ccaggctcaa gttcaacggc cgcgatggcg
      661 ctcctgacgt ggaatacgac gcacgcaagc tgaccggcca ggacggcgac tcctacgctg
      721 acttcgagat cgataaccag gatgcagggc aacgaatcct caacctgcag gactcggtga
      781 agcggaacgt cgcgtttgcc acgctgatcg tcgagagcga cgacaagagc aagggcaagg
      841 gcaaggacaa gggcaaggac agcggcaagt agccggagcg gcgtggggtg cgcgctgagc
      901 gcgcaggagc gcgccgagcg gttccaccag cgacggctgg accaccgacc gggcgcggcg
      961 gtcgaagcgc tcgcgtatcg ccggcggcca ggatgagcgc ggccggcggt cagaaccgat
     1021 aggcgatccg gaggaatccg gtcaggccga tcaccgccgg atcggcggtg gataccgtcg
     1081 tggatcccat catctcgatg tgcgtgaccg ccgtccggtt gaggaacacc gagcccatcg
     1141 cgaaccagtc gcggttgaac cggtagtaca ggttgccgcc gaccgagagg atgctcgagt
     1201 tcgcggcgcc gaagcaggtg ctgaggtcgc tgccgggggg acaggccttg ccggcgctgt
     1261 cgtccttggt cgagctgtag gcgacctcgg cctcccactc gccgcggccg tcggcgagct
     1321 cgcgcgcagc cgacgcgcgc agcgagcgcg acgaggtgcg ctggtaggtc gccgagccga
     1381 cgccgaagat ctgcgagccg tcgatgccga gccgcaggtc cttgaatgac cgccgatcgg
     1441 tgatcgcgcc gtagacctcg acgctctgcg cggcgggcag cgcgatggtc gccggtgccc
     1501 ccgtcatctg cggcggcgcc gacagcgtga catcgccgcg atagcgatac gcgaccgccg
     1561 cggtgacctc gaagcgctgc agctcgccga ggccggccga caggctgccg cggccctcgt
     1621 tggtcgcgat ccgctgcaga taggcctcgt tctggaccac gttgaccttg ggatcggggc
     1681 tggacaggaa cgcctgggcc tggacgttga gggtctcggt gtcgacccgg ttgaagctga
     1741 gcgtgccgcg cagccgcgga tccggcttgt agttgaggcc ggccgacagg ttggtgagcc
     1801 ccgcgttgac cgcgttggag ccgagcagat cgagcaccac gaagtggtag aagtcgagct
     1861 tggtgccata gcgccagtag ccgctggacg tgccgaacac ccgcgggctc tccgccgaca
     1921 gcggcgcgat caccacgccg ccgaacgagc cgtaggcgtc gagcgtgcgg taggcggcgc
     1981 cgaacccgcc cgcgccggtg aaccgcccgg cgtcgttctg atcgttcgac ttcagcggct
     2041 ggtagtcggt ggtgatcgag cgcgagccgc ggatcgggta caacccgccg aacccgagca
     2101 gggtgaactt ggtcgagctc gcgtagtcga cgcgcaaccc gtcgatcttg acgccgccga
     2161 ggtccggcac gaactggcgg ccgaagaaca gatcgctgcg ggtgccgttg cggaccagcc
     2221 acagctcctt gatctccagc tcgttctggc cgaggaagcc ggactggacg ttggtggccg
     2281 accccggggt gaagccgtcg gtgctggcgg tgaccggggc cggggtgttg accaggcggc
     2341 cgcgcacgtc gacccgggcg tcccaccgcg atgcgccgat gtgccggaag tcggtctgca
     2401 ggcgcaggtc ggtgtattcg cggctgaact ggctggccga cggcacgccg gcgggggccg
     2461 gcgcgatcgt cccgccgctc tcgtagtagc cgaacgtcgt cgaggtcagg ctgccctgga
     2521 ccagcgtgcc cttggcggtc ttgtcgctgt cgaccttgtc gacgaagaac cgatcgtgct
     2581 cgaactggtt gtccttcttg ctggtgccga ggtcgtggcc gctcaggtcc tgcttctgga
     2641 gcccggagcc gccggggtcg tcgccgtcgc tcgccttctt cgaggccttc ttcgacgtgc
     2701 cggacgcgtc gtcgtcgtcg atcttgacgt cgtcgccgtc cttgtccttg gcccacgccg
     2761 gaggcgcggt ggccgcgagc acgagtgcga gcgcgagcag gcgcggcacg gtccgcgtca
     2821 tcggcacacc gactcccgac ccagcgcgaa gcccggcgcg ccgagccagg tgttggcgct
     2881 gtggcaattg gcgcacgtcg tcgtcgtgaa gtgtgaattg atcgccccca tcgccacccg
     2941 agcgcccgtg gtgtcgaccg tggtcctgag cgcgtcgtcg tgatggcagc cgacgcattg
     3001 cggcgacagc gcggcgaagt tgccgttctt gtggcagtcg aagcaagcga tcgtgcggtg
     3061 caggccggtg aggttgcagc ccgccttggc gtgatcgaac cgcgccggcg cgaacgacca
     3121 ctgggtgtgg cactcgccgc agcgcgggga cagcgagttg ttgtggatgt cgtcctggcg
     3181 gtggcagttg atgcacaggt tgccgctgcc ggcgagcggc cggttgtcgc ggtggcagcg
     3241 ctcgcacgcg atgttgtcgt gcatgccgcg cagcgagaag tcgccgacgt cgtgcagcgg
     3301 cttgacgtcg ccccacgtcc gggtcgtgtg gcactgcgcg cagccggtgc cgtactggcc
     3361 cttgtggacc gcgggctcgg ggtggcagcc gaagcagtcg gtcggccgcg gcttgaacgt
     3421 caccgagggg tggcagtccg agcaccggac ggtcaggtgc ttgccgtcga gcttgaacgc
     3481 cgacatcgtg ttgtggttga agacgttgtc gccggtctcg acgtggcact gctcgcactt
     3541 gtcgccgagc cggccgcggt gggcgtcgtc ctccttgtgg cagccctcgg ccgcgcaggt
     3601 cgtcggggtc tgggcgaact tgcgctgggg gtgacacgac tcgcacttgt tcttccggtg
     3661 ctcgcccttg agcgggaacg cggtgacctg gcccttggcg tccttgggga acggctggtc
     3721 gtggtcgaac ttgagcgcgt cccacacgcc cgagctgtgg cacgccatgc acttgtcgcc
     3781 gagcgtgccc ttgtgcagcg agtcctcgtg gcactggccg gccgcgttgc agttgggctt
     3841 gaggtcggag aacgaggtct tgcccttctt gtcgcggccg gtgtggcagc tggcgcacgc
     3901 gacgtccttg tggcgcttga ccagcgggaa cgagccggtg ggcccgtgga cctcggcgat
     3961 gatcgagctg cgcttggtgt cgacgttgat gttgccgccg ttgcggtggc actgcaggca
     4021 ctgcgcgctc ttgtacttgc cgtcggggtg ctcgtcgtcg gcgtggacct tggcgtgcgc
     4081 atggcacgac ttgcagtcct tgttggggtt gaacgcgaag cgctcgaagt ccgacgggct
     4141 cgatccgcgg tggcacgcgc ggcaggtgac ctcggcgtgc ttgaactcga gcttgaactt
     4201 ggtgtgcgcg gcgtggttga agttgctggg cgtgaacttg gggccgcccg acgggtggca
     4261 ggtctcgcag cgcggcggcg agccgaactc cttgaaccgg tcgccgtgct tgttgtcctt
     4321 ggcgtggcac tgctcgcacg atcggctggg cttgacctcg ccgagcgcct tggtgtggca
     4381 ctgctcgcac ttgatcttct tgtgcgcggg gccgaggtcg aaccgcgtgc gctcgctgtg
     4441 atcgaacacc tggatgttcg ccaggtcctt gaacgtcggc gagtggcacc actcgcacgg
     4501 ccgcgccccg aacaggtggc cgccgtgcgg gctctggtgg caccccgagt tgccgcacga
     4561 gtcgggcctg gggaacggca gcttgtagac gttcttggcg tggcacttgg tgcacgcgac
     4621 gtccttgtgg cggccgagca gcggcatcgc cgcgtccttg cggtcgtcgt ggttgaactg
     4681 catcgccgtc gccgacttgg ccggcttcca cacgctctcg ccgtggcagc gctcgcaggc
     4741 caggagatcc ttgcgcacga tcttgtgggg ctgatccttg gcgtggcagc tgccgcagcc
     4801 gcgatcggtg cccatgaaca cctgcagccc ctgctccttg ctcctggcct tgtggcagtc
     4861 gttgcagtcg gtggcggcgt gcttgccctt gagctgccag ccggtcagct cgtgatcgaa
     4921 ccccttctgc ccgcccttga ccgacggcca gcccatcaga tcgtaggtcc ggctcttgtg
     4981 ctcgtggtga cacgactcgc acttcttgcc cttgaccatc gacgaggcgt ggaagccctt
     5041 gccggcggcg atccgcgcgc cgaggttctg gtggtcgtgg cagcccaggc acttgtcgtt
     5101 ggacagctcc ttgctgtcgc cgacgtgaca gtcgttgcag tggtcctggg cgtcgagctg
     5161 gccgtggctc ctggtcagcg gcccgggcga gctgccgaag aactggtcgg cgcgcgcggt
     5221 gcgcacgagg gcaagcagca gcaacagcgc ggccagcgcg agcgcgcggc ccaccacacg
     5281 tgtcatgggc ggcatcctac caagcacggg accacgagac ccagatgtgt acggccgaga
     5341 agccggtgag gatcacggtg aacggcacgt gaactttctt ccatgaatgg agcagcagct
     5401 gcgccttcgg cgcgacgacc tggcggcgcg cgatcacgat catccgcgcc gcgcggcgcg
     5461 cgaggtcccg gcggaccctg cgatcgacgc cgagctgcgc gagccggccg cgccgcgcga
     5521 tcgtcgccgg gatccggccg atgtcctgga agatcagcca ccacagcgcg cgggtcacgc
     5581 tcggtgacat cgcgacctcc tgggcccggg cgcgatggat cgcgagctcg cgatcgatct
     5641 cggccatcgc cacggtcagc cgcggccgcg ccttctggaa cgcgcgctcg tggtcgagct
     5701 cctcgagctc gacgccgctc gacagcgccg ggacctgggt gtagaggtag cggccgagga
     5761 agccgctgat cacgacgatc gccatgctcc agaacgccgc cgacacccag gtgtcgagcc
     5821 gcagcgccga gtgcagcacg acgaacatcg gcccgaccgt gccggccatc aggtggaagt
     5881 cgaaccacat cgtgttcgac gccatccagc ggaacagctt gatccggcgg aagatcggat
     5941 agatcgcggc gatgatcatc agcgcggtgc cgatcacgcc gcaccagatc gcaagaggct
     6001 cgccggcacg gacccgcacg ttgtcgagca cgccgctgtc ggcgatgccc tcgtacgtcg
     6061 gcgacgcgcg cagcgtggcg aactggtacg acatggtcgg cgcgtacagc cggagcagga
     6121 cctcgccgag cgcgagcagg aacgcgaaga tcccgagcac ccagatcacg agcggcgcgt
     6181 agcggccgcg cttgacctt
//
LOCUS       DASVOR010001940         1757 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_49997,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001940 DASVOR010000000
VERSION     DASVOR010001940.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49997"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="VF516_36010"
     CDS             <1..504
                     /locus_tag="VF516_36010"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113209.1"
                     /translation="ADEFDLTIFQDLPLDHGLHSPLTMMWPSQPDWPGQIVPLVVNVL
                     QHPVPTPRRCFKLGQALRKAILSYPDDLRVAIVGTGGLSHQMIGDRAGFNNESWDREF
                     LDLIDRDPERLAAMTIDDFRRLGGNEGAEVIMWLIMRGAIAPTARKQHDSYYLPMTTA
                     MAVALFE"
     gene            517..843
                     /locus_tag="VF516_36015"
     CDS             517..843
                     /locus_tag="VF516_36015"
                     /inference="COORDINATES: protein motif:HMM:NF019366.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113210.1"
                     /translation="MAPYIFDLERSARGLRINRFLHSLIEPAVRRRFADDAEQAMRDA
                     GLDDEERRMLRERDWRALMDHGASFFCLEKFARVSGVSNPEMVAGFRGETLEQFLATR
                     RVPGAR"
     gene            840..>1757
                     /locus_tag="VF516_36020"
     CDS             840..>1757
                     /locus_tag="VF516_36020"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF016210.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HEX8113211.1"
                     /translation="MTGALTAQRAIPCLFLRGGTSRGPFFHRADLPADVATRDRVLLA
                     VMGSPDRRQIDGLGGAHPLTSKVGIVARGAAPDDLEFLFAQLQPEGDTVDTTPNCGNM
                     LAAVVPFALETGLVAARGDTSTFRVRTLNTGMACDITVQTPGGRIRYDGAARIDGVPG
                     TAAPILINFLDTAGSVCASLLPTGRAADTVELPGVGPVRVTCIDNGMPMVLVRAAELG
                     RTGDEGVAALDADAELKARLEALRLRCGVHMGLGDVAARSYPKMCLIAAPHHGGSIAT
                     RCFIPHVCHDAIGVLAAVTVATACVLDGTV"
BASE COUNT          238 a          654 c          616 g          249 t
ORIGIN      
        1 gccgacgagt tcgatctgac gatcttccag gacctgccgc tcgaccacgg cctgcactcg
       61 ccgctgacga tgatgtggcc gagccagccc gactggccgg ggcagatcgt gccgctggtg
      121 gtcaacgtgc tgcagcaccc ggtgcccacg ccgcgccggt gcttcaagct cggtcaggcg
      181 ctgcgcaagg cgatcctgtc ctatcccgac gacctgcggg tggcgatcgt cggcaccggc
      241 gggctgtcgc accagatgat cggcgatcgc gccggcttca acaacgagtc gtgggaccgc
      301 gagttcctcg acctgatcga tcgcgacccg gagcgcctgg ccgcgatgac gatcgacgac
      361 ttccgccggc tcggcggcaa cgagggcgcc gaggtcatca tgtggttgat catgcgcggc
      421 gcgatcgccc cgaccgcccg caagcagcac gacagctatt acctgccgat gacgacggcg
      481 atggcggtcg cgctgttcga gtgacatcct gccgccatgg cgccctacat cttcgatctg
      541 gagcgaagcg cgcgggggct gcggatcaac cgcttcctgc actcgctgat cgagccggcg
      601 gtccgccggc ggttcgccga cgacgccgag caggcgatgc gcgacgccgg gctcgacgac
      661 gaggagcgcc ggatgctgcg cgagcgcgac tggcgcgcgc tgatggacca cggcgcgagc
      721 ttcttctgcc tggagaagtt cgcccgggtg agcggtgtgt cgaaccccga gatggtcgcc
      781 gggttccgcg gcgagaccct cgagcagttc ctcgcgaccc gccgcgtgcc gggggcacga
      841 tgactggcgc gctgaccgcg cagcgggcca ttccgtgcct gttcctgcgc ggcggcacct
      901 cgcgcgggcc gttcttccac cgcgccgacc tgccggccga cgtcgcgacc cgcgatcgcg
      961 tgctgctcgc ggtgatggga tcgccggacc gccggcagat cgacggcctc ggcggcgccc
     1021 acccgctgac cagcaaggtc ggcatcgtcg cccgcggcgc tgcgcccgat gacctcgagt
     1081 tcctgttcgc ccagctccag cccgagggcg acaccgtcga caccacgccc aactgcggca
     1141 acatgctcgc cgcggtggtg ccgttcgcgc tggagaccgg cctggtcgcg gcgcgcggcg
     1201 acaccagcac gttccgggtc cgcacgctca acaccggcat ggcgtgcgac atcacggtgc
     1261 agaccccggg cggccggatc cgctacgacg gcgcggcgcg gatcgacggc gtgcccggca
     1321 ccgccgcgcc gatcctgatc aacttcctcg acaccgccgg ctcggtgtgc gcgagcctgt
     1381 tgccgaccgg ccgcgcggcc gacaccgtcg agctgccggg cgtcggcccc gtgcgcgtga
     1441 cctgcatcga caacggcatg ccgatggtcc tggtccgcgc cgccgagctc ggccgcaccg
     1501 gcgacgaggg cgtcgccgcg ctcgacgccg acgccgagct caaggcccgg ctcgaggcgc
     1561 tgcggctgcg ctgcggcgtg cacatgggcc tcggcgacgt cgccgccagg agctatccca
     1621 agatgtgcct gatcgcggcg ccgcaccatg gcggcagcat cgcgacccgc tgcttcatcc
     1681 cgcacgtctg ccacgacgcg atcggcgtgc tggccgcggt gaccgtggcg accgcgtgcg
     1741 tgctcgacgg caccgtg
//
LOCUS       DASVOR010001941         3388 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_108977,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001941 DASVOR010000000
VERSION     DASVOR010001941.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108977"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF516_36025"
     CDS             <1..494
                     /locus_tag="VF516_36025"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEX8113212.1"
                     /translation="PALPGEPPAGGKISPLSVDELYQATIVDHDRAPRNAGVLADPTH
                     QATVDNPLCGDTVTLRARVVDGVIRDIAFESRGCALSRAAASIMTTMVHGRTAEASRE
                     LAAAFERFVQAAPDAAVPDEPNDSEELGELRAFAGVRRFRSRRVCALLGFRALLRALD
                     PAT"
     gene            550..2601
                     /locus_tag="VF516_36030"
     CDS             550..2601
                     /locus_tag="VF516_36030"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8113213.1"
                     /translation="MAITERAPTPHERQTAILERIATNAPLQGTLDALVAHVEAQIPG
                     AIGSLYLLDPDGRHLRCASAHGLPLAYRIASDRVAIGPSEGACGTAAHRREPVFTIDI
                     ASDPLWRARRELAASHGLRACWSTPILSRRRPDRTVPLGTFAVYFRDPAAPEPCHREV
                     LAHAEYLACIALDAEYAIRELRESDARLRMFVDHATDGFFVHDGVTDEVVEANQQACV
                     SLGYARDELIGATTSLFDPYVSREMLDEISRRLASGEIVTFDSRHVRKDGTSFPVEVR
                     IRAFEARGQMFHLALVRDTTARKELEDQLRHAQKMEAVGRLAGGVAHDFNNLLTVING
                     YTDVLLDQVPDDAPEQADLAMIRDAGDRAAALTAQLLAFSRRTIIAPKALNLNILVER
                     LVKMCKRIIGENIVLTTHLDPDLDPVMADAGQIEQVVMNLVVNARDAMPRGGRLSLTT
                     SNYRALTGSHVRLTVTDTGEGMTDEVRARVFEPFFTTKPQGQGSGLGLATAYGIVEQA
                     NGQIRVESTPGKGSSFSVILPVIDAAESSSPLVTRDRPRGTETVLLAEDEDAVRRLVQ
                     RALELHGYSVLPARSGEEAEQIERAHLGPIHLLITDVVMPGMGGRELADTIKSRRPQL
                     KILYMSGYTNDEVVRHGVTLARDAFLQKPFTPSGLVAMVRAAFAGHASPGELASSSEL
                     G"
     gene            complement(2640..>3388)
                     /locus_tag="VF516_36035"
     CDS             complement(2640..>3388)
                     /locus_tag="VF516_36035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113214.1"
                     /translation="VAVSNYTRKHRVTGVAEYSYPLIGKLVDDAPWKNMKTSFGLFVE
                     ARSGQPYSWTFGGRNDNGTTDNTGQTLSRIFGEDSTLASRNRELFYVPLDSEVCETTY
                     VAGCKVILRTGASGPLTRAQFNDFLDRTGLSAYRGKIAPRNAFAGPRYARWDVRFAQD
                     LPNPMVGQRARIVLDIENFGNLLNSSWGIFRQVPFPYYQPALDVGVDRVNNNAYIYSN
                     LRSPNPTNGPSTTDVLLSVWRLSLGVMYDF"
BASE COUNT          527 a         1210 c         1166 g          485 t
ORIGIN      
        1 ccccggcttt gccgggggag ccgccagccg gcgggaaaat cagcccccta tctgtagacg
       61 agctgtacca ggccaccatc gtcgaccacg atcgcgcacc gcgcaacgcc ggggtcctgg
      121 ccgacccgac ccaccaggcc acggtcgaca atccgctgtg cggcgatacc gtcacactcc
      181 gcgcccgggt cgtggacggg gtgatccgag acatcgcgtt cgagagccgc ggctgcgcgc
      241 tgtcccgcgc cgcggcgtcg atcatgacca cgatggtgca cggccgcacg gccgaggcat
      301 cgcgcgagct cgccgccgcg ttcgagcggt tcgtccaggc cgcgcccgac gccgcggtcc
      361 ccgacgaacc gaacgacagc gaggaactcg gcgagctgcg cgcgtttgcc ggcgtacgta
      421 gattccgctc gcgccgggtg tgcgccttgc tcggctttcg cgcactgctg cgcgccctgg
      481 acccggcaac gtgagggtca actacatacc tgttggcgat ggcctgatcg gaccaacgta
      541 atggggagta tggcgatcac cgagcgggca ccgacgccgc acgagcgaca gaccgcgatc
      601 ctggaacgca ttgcgaccaa cgctccactg cagggcacgc tcgatgcgct ggtcgcccac
      661 gtcgaggccc agatcccggg cgcgatcggc tcgctctacc tgctcgaccc cgacggtcgt
      721 cacctgcgct gcgcgtcggc ccacgggctg ccgctggcct accgcatcgc atccgaccgg
      781 gtcgcgatcg gcccgagcga gggcgcgtgc gggaccgcgg cgcaccgccg cgagccggtg
      841 ttcacgatcg acatcgcgag cgatccgctg tggcgcgcgc gccgcgagct cgccgccagc
      901 cacggcctgc gcgcgtgctg gtcgacgccg atcctgtcgc gccgccggcc ggatcgcacc
      961 gtgccgctcg ggaccttcgc ggtctacttc cgcgatccgg ccgcgcccga gccatgccat
     1021 cgcgaggtgc tcgcgcacgc cgagtacctg gcgtgcatcg cgctcgacgc cgagtacgcg
     1081 atccgcgagc tgcgcgagag cgacgcgcgg ctgcggatgt tcgtcgacca cgcgaccgac
     1141 gggttcttcg tccacgacgg cgtgaccgac gaggtcgtcg aggccaacca gcaggcctgc
     1201 gtcagcctgg ggtacgcccg cgacgagctg atcggcgcga cgaccagcct gttcgatccg
     1261 tacgtgtcgc gcgagatgct cgacgagatc tcgcggcggc tcgcgagcgg cgagatcgtc
     1321 acgttcgact cgcgccacgt ccgcaaggac ggcacgtcgt tcccggtcga ggtccggatc
     1381 cgggcgttcg aggcccgcgg ccagatgttc cacctcgcgc tggtccgcga caccaccgcg
     1441 cgcaaggagc tcgaggacca gctccgccac gcccagaaga tggaggcggt cggccggctc
     1501 gccggcggcg tcgcgcacga cttcaacaac ctgctcaccg tgatcaacgg ctacaccgac
     1561 gtgctgctcg atcaggtccc ggacgacgcg cccgagcagg ccgatctcgc gatgatccgc
     1621 gacgccggcg accgcgcggc ggcgctgacc gcccagctgc tggcgttcag ccggcggacc
     1681 atcatcgcgc ccaaggcgct caacctcaac atcctggtcg agcgcctggt caagatgtgc
     1741 aagcggatca tcggcgagaa catcgtgctc accacccacc tcgatccgga tctcgatccg
     1801 gtgatggccg acgccgggca gatcgagcag gtggtgatga acctggtggt caacgcgcgc
     1861 gacgcgatgc cgcgcggcgg ccggctctcg ctcacgacca gcaactaccg cgcgctgacc
     1921 ggcagccacg tccggttgac cgtgaccgac accggcgagg gcatgaccga cgaggtccgc
     1981 gcccgggtgt tcgagccgtt cttcaccacc aagccgcagg gccagggctc ggggctcggg
     2041 ctggccaccg cgtacggcat cgtcgagcag gccaacggcc agatccgggt cgagagcacg
     2101 cccggcaagg gcagctcgtt cagcgtcatc ctgccggtga tcgacgcggc ggagtcgagc
     2161 tcaccgctgg tcactcgcga ccggccgcgc ggcaccgaga ccgtgctgct cgccgaggac
     2221 gaggacgccg tgcgccggct ggtccagcgc gcgctggagc tccacggcta cagcgtgctg
     2281 ccggcgagga gcggcgagga ggccgagcag atcgagcgcg cgcacctcgg gccgatccac
     2341 ctgctgatca ccgacgtcgt gatgcccggg atgggcggcc gcgagctggc cgacaccatc
     2401 aagtcgcgcc ggccgcagct caagatcctc tacatgagcg gctacaccaa cgacgaggtc
     2461 gtccgccacg gcgtgaccct cgcgcgcgac gcgttcctgc agaagccgtt cacgccgagc
     2521 ggcctggtcg ccatggtgcg agcggccttc gccggtcacg ccagtcccgg cgagctcgcc
     2581 agttccagcg agctcggctg atgtccgcag cgctcacgcc gcagtagccc gcccgccgat
     2641 cagaagtcgt acatcacgcc gaggctcagc cgccacaccg agagcaggac gtccgtggtg
     2701 gacggaccgt tggtcgggtt gggcgaccgc aggttcgaat agatgtacgc gttgttgttg
     2761 acgcgatcca cgccgacgtc gagcgccggc tggtagtacg ggaacggaac ctggcggaag
     2821 atgccccagc tgctgttgag caggttgccg aagttctcga tgtcgaggac gatccgggcg
     2881 cgctgcccca ccatcgggtt gggcagatcc tgcgcgaacc ggacgtccca gcgtgcgtag
     2941 cgtgggccgg cgaacgcgtt gcgcggcgcg atcttgccgc ggtacgccga caggccggtg
     3001 cggtcgagga aatcgttgaa ctgcgccctg gtgagcgggc cgctggcgcc ggtcctcagg
     3061 atcaccttgc agcccgccac gtaggtggtc tcacagacct cggagtcgag cgggacatag
     3121 aacagctcgc ggttgcggct cgccagcgtc gagtcctcgc cgaagatgcg cgacagggtc
     3181 tggccggtgt tgtcggtggt gccgttgtcg ttgcggccgc cgaacgtcca cgagtacggc
     3241 tgacccgacc gggcctcgac gaacaggccg aagctggtct tcatgttctt ccacggcgcg
     3301 tcgtcgacga gcttgccgat cagcgggtac gagtactcgg ccacgccggt gacccggtgc
     3361 ttccgcgtgt agttcgagac cgcgacat
//
LOCUS       DASVOR010001942         2416 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_371176,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001942 DASVOR010000000
VERSION     DASVOR010001942.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2416
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371176"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2416
                     /locus_tag="VF516_36040"
     CDS             <1..>2416
                     /locus_tag="VF516_36040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113215.1"
                     /translation="RLVGIVEERGGRLDALRANLEDRPGHEPSIDAVARWAVDALVDL
                     YRTGGRARAAVDTLVEAARLPFDQNTRRDMRLRAAQLATVELGDNAAAIDMYRSVLAQ
                     SPSDLEVLDRLAHLLGLEDRVPELLTLRQIQLGLEPDAEKKLGLRLELARLVGIVEER
                     GGRLDALKANLEDRPGHEPSIEAVAELLAGKGQHRALADLLEQQAQRLESAGDTARAA
                     KLWARYAQVAETDTREVERAIAGHRRVVALAPTSDSLRALARLNLERSQPGQAVPWLE
                     SLLGTVPAGERLHVVSQLAKAHLSANQPDRAIAAIEVNLDDKEPAIELRILLADLYRK
                     SEAWEPLARHLTRSLPLVKDDRLARELAREASSIYIHKLSAPAKAIPALETALQLDPT
                     DKDLRTALAIGQRVAGKLPEARAALAELINDFGRRRSPERAALHVELARVAQAEGKLD
                     EAMAEMESASKMDVSNAAIQKELAEMARTAGQLDKAERTYRALLLVVRRQPPGDDEAA
                     VGASEVLFELHKLAASRGETSDQAKELLESAMEAAIQSDAEVRRLRRSLIAHGEGELL
                     LQVLERRLATNPDLHSQARLLGDIAETLDGQLGRHDEALDALIRAIGVMPSRTDLHER
                     ARELARRTGQTKRFVDAVEAVVDRLRRKDDPPLIANLLMRAGEALEQDAGDLRAAANL
                     YRRVEILGERLAEAFYAQARVAAALGDTAEQARTLDKMFELAGTDAEPTPAQVDALYR
                     LAEIFLGSDARRRQGFDLLERAFAAEPRWAQAGRLLRQAAAAADADDRVLQMYERVAR
                     NGGDAEL"
BASE COUNT          339 a          951 c          873 g          253 t
ORIGIN      
        1 cgcgcctggt cggcatcgtc gaggagcgcg gcggccggct cgacgcgctg cgcgccaacc
       61 tggaggaccg cccgggccac gagccgtcga tcgacgcggt cgcgcgctgg gcggtcgacg
      121 cgctggtcga cctctaccgc accggcggcc gcgcccgcgc cgcggtcgac accctggtcg
      181 aggccgcgcg gctgccgttc gaccagaaca cccggcgcga catgcggctg cgcgccgccc
      241 agctggcgac cgtcgagctc ggcgacaacg ccgccgcgat cgacatgtac cgcagcgtgc
      301 tcgcccagtc gcccagcgac ctcgaggtgc tcgaccggct cgcccacctg ctcggcctcg
      361 aggaccgcgt ccccgagctg ctcacgctgc gccagatcca gctcgggctc gagccggatg
      421 ccgagaagaa gctcgggctg cggctcgagc tcgcgcggct ggtcggcatc gtcgaggagc
      481 gcggcggccg gctcgacgcg ctcaaggcca acctcgagga ccggccgggc cacgagccgt
      541 cgatcgaggc ggtcgccgag ctgctcgccg gcaagggcca gcaccgcgcg ctcgccgacc
      601 tgctcgagca gcaggcccag cggctggaga gcgcgggcga caccgcgcgc gccgccaagc
      661 tgtgggcgcg ctacgcccag gtcgccgaga ccgacacccg ggaggtcgag cgcgcgatcg
      721 ccggccaccg ccgcgtcgtc gcgctggcgc cgaccagcga ctcgctgcgc gcgctcgccc
      781 ggctcaacct cgagcgcagc cagcccggcc aggcggtgcc gtggctcgag agcctgctcg
      841 gcaccgtgcc ggccggcgag cggctgcacg tcgtgtcgca gctcgccaag gcccacctgt
      901 cggccaacca gcccgaccgc gcgatcgccg cgatcgaggt caacctcgac gacaaggagc
      961 cggcgatcga gctgcgcatc ctgctcgccg acctgtaccg caagtccgag gcctgggagc
     1021 cgctggcccg ccacctcacg cgcagcctgc cgctggtcaa ggacgacagg ctcgcccgcg
     1081 agctggcgcg cgaggcgtcg agcatctaca tccacaagct cagcgcgccg gccaaggcga
     1141 tcccggcgct cgagaccgcg ctccagctcg atccgaccga caaggacctg cggacggcgc
     1201 tggcgatcgg ccagcgcgtc gccggcaagc tccccgaggc ccgcgccgcg ctggccgagc
     1261 tgatcaacga cttcggccgc cggcgctcgc ccgagcgcgc cgcgctccac gtcgagctcg
     1321 cccgggtcgc ccaggccgag ggcaagctcg acgaggcgat ggccgagatg gagtccgcgt
     1381 ccaagatgga cgtgtccaac gcggcgatcc agaaggagct cgccgagatg gcgcgcacgg
     1441 ccggccagct cgacaaggcc gagcggacct accgcgcgct cctgctcgtc gtccgccgcc
     1501 agccgccggg cgacgacgag gccgcggtcg gcgccagcga ggtgctgttc gagctccaca
     1561 agctcgcggc gagccgcggg gagaccagcg accaggccaa ggagctgctc gagtcggcga
     1621 tggaggcggc gatccagtcc gacgccgagg tccgccggct gcgccgctcg ctgatcgcgc
     1681 acggcgaggg cgagctcctg ctccaggtgc tcgagcgccg gctcgcgacc aaccccgacc
     1741 tgcacagcca ggcccggctg ctcggcgaca tcgccgagac gctcgacggc cagctcggcc
     1801 gccacgacga ggcgctcgac gcgctgatcc gcgcgatcgg cgtcatgccg tcgcgcaccg
     1861 acctgcacga gcgcgcccgc gagctcgccc ggcggaccgg gcagaccaag cggttcgtcg
     1921 acgcggtcga ggcggtggtc gatcgcctgc gccgcaagga cgatccgccg ctgatcgcca
     1981 acctcctgat gcgcgccggc gaggcgctcg agcaggacgc cggcgacctg cgcgccgcgg
     2041 ccaacctgta ccgccgggtc gagatcctcg gcgagcggct cgcggaggcg ttctacgccc
     2101 aggcccgggt cgccgccgcg ctcggcgaca ccgcggagca ggcgcgcacg ctcgacaaga
     2161 tgttcgagct cgccggcacc gacgccgagc cgaccccggc ccaggtcgac gcgctgtacc
     2221 ggctcgccga gatcttcctc ggcagcgacg cccgccgccg ccagggcttc gacctgctcg
     2281 agcgcgcgtt cgccgccgag ccgcgctggg cccaggccgg gcggctgctg cgccaggccg
     2341 cggcggcggc cgatgccgac gaccgggtgc tgcagatgta cgagcgggtc gcgcgcaacg
     2401 gcggcgacgc cgagct
//
LOCUS       DASVOR010001943         6126 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_109017,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001943 DASVOR010000000
VERSION     DASVOR010001943.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6126
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109017"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..1644)
                     /locus_tag="VF516_36045"
     CDS             complement(70..1644)
                     /locus_tag="VF516_36045"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEX8113216.1"
                     /translation="MAPEPAAPGARSADAEAGTDALRLRASALYLNVFVVAICGLVYE
                     LLAGTLGSYLLGDSVTQFSLVIGLYLSAMGAGAWLSRRITGELGRRFIEIELAVAVVG
                     GVSAPVLFLAFGSVAHFQVVLLGFVAAIGVLVGLELPLLMRLLEGQVAFKDLVSRVLT
                     FDYIGALAAAVMFPLVLVPKLGLVRSSLAIGAANACVGLWGTWLLADQLGAGRRGLRL
                     RALAVLVVMAAALAIAGRFTTWAEDEMYADPVVFSETTPYQRIVVTRGRAGFQLFLNG
                     NLQFASADEYRYHEALVHPAMMAAEAPPRRALILGGGDGLALREVLAHPSILAVTLVD
                     LDPAMTDLARRFPPLGELNHHAFADRRVTVINDDAMVWLDEHAGTWDVIIVDFPDPNT
                     FALGKLYTRLFYRRLLARLAPGGAVSVQATSPLFARRSYWCIIETMRSAGFTVRPYHV
                     TVPSFGVWGYALARKAPFPPPIQVPDVPLRFLNRPALAAMFELPADMTPVAVDINQLD
                     NQALVRYYEKEWRRWE"
     gene            complement(1634..1855)
                     /locus_tag="VF516_36050"
     CDS             complement(1634..1855)
                     /locus_tag="VF516_36050"
                     /inference="COORDINATES: protein motif:HMM:NF015927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HEX8113217.1"
                     /translation="MKIENLGQAAIDTAVFAAIGLAVFGIAFWLIVKIAPFSVKKEIE
                     DDQNIALAIIIAGVLIGISLIIAAAISGT"
     gene            complement(1855..2106)
                     /locus_tag="VF516_36055"
     CDS             complement(1855..2106)
                     /locus_tag="VF516_36055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113218.1"
                     /translation="MTEAVPPGAPAPDLPPVPRPKRSAMAIVLGMFGALVTAGYAALA
                     YSDYEPGSPERDEIPASVRSSPGGYRSFHFWHSGYHGGK"
     gene            complement(2099..4081)
                     /locus_tag="VF516_36060"
     CDS             complement(2099..4081)
                     /locus_tag="VF516_36060"
                     /inference="COORDINATES: protein motif:HMM:NF025158.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4178 domain-containing protein"
                     /protein_id="HEX8113219.1"
                     /translation="MIVGCPTCGAEVEFRYDDSFVRVCGHCRAAVVRTDRGVETLGRF
                     ADLVPIASPLRLFAEGRQGSTGFLLIGMAQLRHAAGGVWQEWYAKLDGGRWAWISEAQ
                     GRLYFTLERPDVAAPPISALAQLAPGARIALAGTTFTVAERGTATYVSALGEIPYRLV
                     PSSSFQFVDLSDGRGGFATIDYGDGSEPPAVYIGYLVTPESLGLSGGEAPAEAQAGPA
                     QGARLACPNCNGALELRAPDQTLRVACPYCGSLVSVEGGALSILARQARKPRLAIALG
                     SKGRFADGELTVIGYVGRSAYVDAQWWPFEEYLLYAPGVGFRWLVCSDGHWSYVQPVA
                     AGAVSAGVDARYGGVAFQRFQIATLRVDSVLGEFYWRVEAGERVESTDFVAPPAMLSC
                     EASRAEQNWSLSTYLAPRDVERAFGAPLGLPPPVGVGPNQPYPGGVGRAILLVLAVFF
                     AVGIAKCASAPSAEKLVQDFTVSMQSQAPRPAVDPTLGAAADGSGDAAGEPPGTVLFS
                     DKFELDGGRNVAIDLDANVYNNWCYAAIDLVNEATGQVVSFDANLEYYAGVDDGESWD
                     EGSHTTQQVIGPVEPGGYVLRVEAQHGGTGDVRLRVIVRQGVFRGLWFWLGLFVLAVP
                     FTVIGLHAARFRARRWQNSNITRSGARPHLPGGDDD"
     gene            complement(4078..4422)
                     /locus_tag="VF516_36065"
     CDS             complement(4078..4422)
                     /locus_tag="VF516_36065"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEX8113220.1"
                     /translation="MHGGIEWLVDAHDCVPGKLRDRAAVLALLDRIVAAMDLHVVSTA
                     VHVFPGAGGVTALYLLAESHLTIHTFPETATATLNAYCCRPREPAPWRALCGELLGAR
                     QVTATEHRRGRP"
     gene            complement(4481..5224)
                     /locus_tag="VF516_36070"
     CDS             complement(4481..5224)
                     /locus_tag="VF516_36070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113221.1"
                     /translation="MRAVLTVAALVIAARPVAADPTGSRVLTAPTAWLPPPDSATATL
                     GIDHRGDTAAFVSASLDGLAELEIDEDSDVRGCTDCTMPHTPLHLGRAAFRIGAHQDA
                     WLHGMPAVVLGVRATFAAYQPAVGAPRATDAYLVASRDLGVVRLHAGVDALSASVAGH
                     RSAAELRPLAGVELHPPMYPRSSLLGDIAWEPELDAAQGPALRWMLGIGVRYQAFSWG
                     SVELAVRARQNDDLGGATVMVRFNAIARR"
     gene            complement(5227..>6126)
                     /locus_tag="VF516_36075"
     CDS             complement(5227..>6126)
                     /locus_tag="VF516_36075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113222.1"
                     /translation="GPDPGQPGLPGQVVTQPDGSVELTPTPPPARPGPPPRVHKMRFP
                     EVLNTPTGWLLPAGVLYSRNAIDTGGGVSSDNRVGLGDVAEFGIATTDQVRERQIDST
                     KEEAKVIQPYVTASFRLGVDENRLFKGQPGVTLGFRKSFEREHAGSKTRIAELTLVAS
                     KHFGPDVAFHLGGSVWDASLQQNADAAMPPDPVTLHGHGLARQIRAFGGFEARPLPRS
                     QILIDLSWAPEFCYGCTVDTDKIKLKPELAWGVRYQVASFMHIESGVRVPDIGNANLL
                     DAQIFGRITLTSFALSDAVETLK"
BASE COUNT          919 a         2308 c         2162 g          737 t
ORIGIN      
        1 gcgcccgccc gctgcacgcc agcgcgagcg gcgcgccgag caacagcgcc acgaactcgc
       61 gccgcgagct cactcccacc gccgccactc cttctcgtag tagcggacca gcgcctggtt
      121 atcgagctgg ttgatgtcga ccgcgaccgg ggtcatgtcg gccggcagct cgaacatcgc
      181 cgcgagcgcc ggccggttga ggaaccgcag cggcacgtcc ggcacctgga tcggcggcgg
      241 gaacggcgcc ttgcgcgcca gcgcgtagcc ccacacgccg aacgacggca cggtgacgtg
      301 gtacggccgg acggtgaacc ccgccgagcg catggtctcg atgatgcacc agtacgaccg
      361 ccgcgcgaac aggggcgacg tcgcctggac gctgaccgcg ccgcccgggg ccagccgcgc
      421 cagcagccgg cggtagaaca gccgggtgta gagcttgccg agcgcgaacg tgttcggatc
      481 cgggaagtcg acgatgatca cgtcccacgt cccggcgtgc tcgtcgagcc acaccatcgc
      541 gtcgtcgttg atcacggtga cccggcgatc ggcgaacgcg tggtggttga gctcgccgag
      601 cggcgggaac cggcgcgcca ggtcggtcat cgccggatcg agatcgacca gcgtcaccgc
      661 caggatcgac gggtgcgcca gcacctcgcg cagcgccagg ccgtcgccgc cgccgaggat
      721 cagcgcgcgc cgcggcggcg cctcggccgc catcatcgcc gggtggacca gcgcctcgtg
      781 gtagcggtac tcgtcggccg acgcgaactg caggttgccg ttgaggaaca gctggaaccc
      841 ggcgcggccg cgggtcacca cgatgcgctg gtacggcgtg gtctcgctga acaccaccgg
      901 atcggcgtac atctcgtcct cggcccaggt cgtgaagcgg cccgcgatcg cgagcgccgc
      961 cgccatcacg accagcaccg cgagcgcgcg gagccggagg ccgcgccggc ccgccccgag
     1021 ctggtcggcg agcagccagg tgccccacag cccgacgcac gcgttggccg cgccgatcgc
     1081 cagcgacgac cgcaccagcc cgagcttggg caccagcacg agtgggaaca tcaccgccgc
     1141 ggcgagcgcg ccgatgtagt cgaacgtcag gacccgcgac accaggtcct tgaacgcgac
     1201 ctggccctcg agcagccgca tcagcagcgg aagctcgagc ccgaccagca cgccgatcgc
     1261 ggcgacgaac ccgagcagca cgacctggaa gtgcgcgacc gagccgaacg ccaggaacag
     1321 gaccggcgcc gacaccccgc cgaccaccgc gaccgcgagc tcgatctcga tgaaccggcg
     1381 gccgagctcg ccggtgatcc gccgcgacag ccacgcgccg gcgcccatcg ccgacaggta
     1441 cagcccgatc accagcgaga actgggtgac cgagtcgccg agcaggtagc tgccgagcgt
     1501 gccggcgagc agctcgtaga ccaggccgca gatcgcgacc acgaacacgt tgaggtacag
     1561 cgcgctcgcc cgcagccgga gcgcgtcggt tccggcctcg gcgtcggcgc tgcgggcgcc
     1621 gggggccgcg ggctcaggtg ccactgatcg ccgctgcgat gatgagcgag atcccgatca
     1681 gcacgcccgc gatgatgatc gcgagcgcga tgttctgatc gtcctcgatc tccttcttca
     1741 ccgagaacgg cgcgatcttc acgatcagcc agaacgcgat cccgaacacg gccagcccga
     1801 tcgccgcgaa caccgcggtg tcgatcgcag cctggcccag gttctcgatc ttcatcactt
     1861 gcctccgtgg tagccgctgt gccagaaatg aaacgaacga tagccgccgg gcgacgatcg
     1921 cacgctcgcc gggatctcgt cgcgctcggg cgagcccggc tcgtagtcgc tgtaggccag
     1981 cgcggcgtag ccggcggtga ccagcgcgcc gaacatgccg agcacgatcg ccatcgccga
     2041 ccgcttcggc cgcggcaccg gcggcagatc gggcgccggt gcgccggggg gtacagcttc
     2101 agtcatcgtc gcctccaggg agatgcggcc gggcgcccga ccgcgtgatg ttgctgttct
     2161 gccaccgccg cgcgcggaac cgcgccgcgt gcagcccgat cacggtgaac gggacggcga
     2221 gcacgaacag cccgagccag aaccacagcc cgcggaacac gccctggcgc acgatgacgc
     2281 gcagccggac gtcgccggta ccgccgtgct gggcctcgac ccgcagcacg tagccgcccg
     2341 gctcgaccgg cccgatcacc tgctgggtgg tgtgcgagcc ctcgtcccac gactcgccgt
     2401 cgtcgacgcc ggcgtagtac tcgaggttcg cgtcgaagct gaccacctgg ccggtggcct
     2461 cgttgaccag atcgatcgcg gcgtagcacc agttgttgta gacgttggcg tcgagatcga
     2521 tcgcgacgtt gcggccgccg tcgagctcga acttgtccga gaacaggacg gtgcccggcg
     2581 gctcgcccgc cgcgtcgccc gacccgtccg ccgcggcgcc gagcgtcgga tcgaccgccg
     2641 gccgcggcgc ctgggactgc atcgacaccg tgaagtcctg gaccagcttc tcggccgacg
     2701 gcgcgctcgc gcacttggcg atgccgaccg cgaagaacac ggccaggacc agcaggatcg
     2761 cccggccgac cccgccgggg tacggctggt tcggcccgac cccgaccggc ggcggcaggc
     2821 cgagcggcgc gccgaacgcg cgctcgacgt cgcgcggcgc caggtaggtc gacagcgacc
     2881 agttctgctc cgcccgcgac gcctcgcagg acagcatcgc cggcggcgcg acgaagtcgg
     2941 tcgactccac ccgctcgccg gcctcgaccc gccagtagaa ctcgccgagc acgctgtcga
     3001 cccgcagcgt ggcgatctgg aaccgctgga atgccacgcc gccgtagcgc gcgtcgaccc
     3061 cggccgacac cgcgccggcc gccaccggct ggacgtagct ccagtggccg tcggagcaca
     3121 ccagccagcg gaaccccacg cccggcgcgt acagcaggta ctcctcgaac ggccaccact
     3181 gggcgtcgac gtacgcgctg cgcccgacgt agccgatcac ggtcagctcg ccgtcggcga
     3241 accggccctt gctgccgagc gcgatcgcga gccgcggctt cctggcctgg cgcgcgagga
     3301 tcgacagcgc gccgccctcg acgctgacca ggctgccgca gtacgggcac gcgacccgca
     3361 gcgtctgatc cggcgcccgg agctcgagcg cgccgttgca gttcgggcac gcgagccgcg
     3421 cgccctgcgc cggaccggcc tgggcctccg ccggcgcctc gccgcccgac agcccgaggc
     3481 tctcgggcgt gaccaggtag ccgatgtaca cggccggcgg ctcgctgccg tcgccgtagt
     3541 cgatcgtcgc gaacccgccg cgcccgtcgg acagatcgac gaactggaag ctcgagctcg
     3601 gcaccaggcg atacgggatc tcgccgagcg ccgagacgta cgtcgcggtg ccgcgctcgg
     3661 ccacggtgaa cgtcgtcccg gcgagcgcga tccgcgcgcc gggcgcgagc tgggccagcg
     3721 ccgagatcgg cggcgccgcg acgtcgggcc gctccagcgt gaagtacagc cggccctgcg
     3781 cctcggagat ccacgcccac cgcccgccgt cgagcttggc gtaccactcc tgccacaccc
     3841 cgccggccgc gtggcggagc tgcgccatgc cgatcagcag gaacccggtc gagccctggc
     3901 ggccctcggc gaacagccgc agcggcgagg cgatcggcac caggtcggcg aaccggccga
     3961 gcgtctcgac gccgcgatcg gtgcgcacca ccgcggcgcg gcagtggccg cacacccgga
     4021 cgaacgagtc gtcgtagcgg aactcgacct cggcgccgca ggtcgggcag ccgacgatca
     4081 cggccgaccc cgccggtgct cggtcgcggt gacctgccgc gcacccagca gctcgccgca
     4141 cagcgcgcgc cacggcgccg gctcgcgcgg ccggcagcag taggcgttga gcgtggcggt
     4201 ggcggtctcg gggaacgtgt ggatcgtcag gtgcgactcg gcgagcaggt acagcgcggt
     4261 gaccccgccg gcgccgggga acacgtgaac cgcggtcgac accacgtgga ggtccatcgc
     4321 ggcgacgatc cggtcgagca gcgcgagcac cgcggcgcga tcgcgcagct tgccggggac
     4381 gcagtcgtgc gcgtcgacca gccactcgat gccgccgtgc attcgaccaa gcgtagtagc
     4441 ccgccgcgcc gcgctcaagc cccggcgccg cgatcgccgg tcagcgccgc gcgatcgcgt
     4501 tgaaccggac catgacggtc gcgccgccga ggtcgtcgtt ctgccgcgcc cgcaccgcga
     4561 gctcgaccga tccccacgag aacgcctggt agcgcacccc gatcccgagc atccagcgca
     4621 gcgccgggcc ctgcgccgcg tcgagctcgg gctcccacgc gatgtcgccg agcaggctcg
     4681 accgcgggta catcggcggg tggagctcca cgccggccag cggccggagc tcggcggccg
     4741 agcgatggcc ggcgacgctc gcggacagtg cgtcgacccc ggcgtgcagc cggaccaccc
     4801 cgagatcgcg gctcgccacc aggtacgcgt cggtcgcccg gggcgcgccg accgccggct
     4861 ggtaggccgc gaacgtcgcg cgcacgccca gcaccaccgc cggcatgccg tgcagccacg
     4921 cgtcctggtg ggcgccgatc cggaacgccg cgcgccccag gtgaagcggc gtgtgcggca
     4981 tcgtgcagtc ggtgcagccc cgcacgtcgc tgtcctcgtc gatctcgagc tcggccagcc
     5041 cgtcgagcga ggcgctgacg aacgccgcgg tgtcgccgcg gtgatcgatc cccagcgtcg
     5101 cggtcgccga gtccggaggc ggcagccagg ccgtcggcgc ggtgagcacg cgcgaccccg
     5161 tcggatcggc ggccaccggc cgggccgcga tcaccagtgc ggccaccgtg agcaccgcgc
     5221 gcatggctac ttcagcgttt ccaccgcgtc gctcagcgcg aacgacgtga gcgtgatccg
     5281 gccgaagatc tgggcgtcga gcaggttggc gttgccgatg tcggggacgc gcacgccgga
     5341 ctcgatgtgc atgaagctcg cgacctggta gcgcacgccc cacgccagct cgggcttgag
     5401 cttgatcttg tcggtgtcga ccgtgcagcc gtaacagaac tcgggtgccc agctgaggtc
     5461 gatcaggatc tgcgagcgcg gcagcgggcg ggcctcgaac ccgccgaacg cgcggatctg
     5521 ccgggcgagg ccatggccgt gcagcgtgac cggatccggc ggcatcgcgg cgtcggcgtt
     5581 ctgctgcagc gaggcgtccc agaccgagcc accgaggtgg aacgcgacgt cgggcccgaa
     5641 atgcttgctc gcgaccagcg tcagctcggc gatccgggtc ttcgatccgg cgtgctcgcg
     5701 ctcgaacgac ttgcggaacc cgagcgtcac gccgggctgg cccttgaaca gccggttctc
     5761 gtcgaccccg agccggaacg acgccgtgac gtagggctgg atgaccttgg cctcctcctt
     5821 ggtgctgtcg atctggcgct cgcgcacctg atcggtggtc gcgatcccga actcggcgac
     5881 gtcgcccagg ccgacccggt tgtcgctcga caccccaccg ccggtgtcga tcgcgttgcg
     5941 cgagtacagc acgccggccg gcagcagcca cccggtcggc gtgttcagca cctcggggaa
     6001 ccgcatcttg tgcacgcgcg gcggcgggcc cggcctggcg ggcggcggag tcggcgtgag
     6061 ctcgaccgac ccgtcgggct gcgtcaccac ctggccgggc agcccgggct ggccgggatc
     6121 cgggcc
//
LOCUS       DASVOR010001944         2306 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_137358,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001944 DASVOR010000000
VERSION     DASVOR010001944.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137358"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2135
                     /locus_tag="VF516_36080"
     CDS             <1..2135
                     /locus_tag="VF516_36080"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8113223.1"
                     /translation="AASPATPAPAPAARAGLSYPAAHTGDVVETHHGVAVPDPYRWLE
                     DMSSAETRQWVAAENAVTDAYLAGLPGRDALRQRIGELISYESFGPPARHGARYFWTH
                     RDGKQSQPVVFTAPGLDAAPGVLLDPNAISTDGSLAFIGLAVNHAGTRIAYGLAHGGG
                     DWQTWRVRDVAGGKDLPDELDHIKYYRPVFTHDGTGVFYSRFPAPPPGKELVETDHDC
                     KLYYHRIGTPAAADLVVYERPDHPTWQFDPVITDDGRYLVITTGDGQVGDRGVELVGY
                     VDLERPGQKPAPGKPAAPTPLVDTYDAEYRFLGNDGPVFYFQTTLGAANKRIIAIDTR
                     HPARDRWQEVVAEGPNAIQNATVVGRQLIVTTLQDAHHAVVAYDLHGKKLRDLELPGI
                     GSANGFGGDPDARETFYLFNGFTVPGTVYRYELATGKSTPWKAPHVAFDSASFETTQV
                     FFPSKDGTKVPMFLTAKKGLPRDGTSPTILYAYGFGGISSTPRFDPARIAWLERGGIW
                     AMVNIRGGGEYGEAWHNAARRAHRQVAYDDFLAAGDWLVANKVTSTAHLGATGSSGGG
                     MLVAGALVQRPDLFAAVLPFAGVHDLLRFHLFGQGAGWQGDLGSIDDPTEFAALRATS
                     PLHNVRSGTRYPAVLLITADHDVRVAPLHSYKLAAALQAAQAGPAPVVLRVETASGHG
                     GGTTRAQAIEQQTERYMFFAANLGLRVD"
BASE COUNT          329 a          910 c          758 g          309 t
ORIGIN      
        1 cggccgcatc gcccgcgacc ccagcgccgg caccggcggc gcgcgccggt ttgagctacc
       61 cggcggcgca caccggcgac gtcgtcgaga cccaccatgg cgtggcggtc cccgatccgt
      121 accgctggct cgaggacatg agctcggcgg agacccgcca gtgggtcgcc gccgagaacg
      181 ccgtgaccga cgcctacctc gccgggctgc ccggccgcga cgcgctgcgc cagcggatcg
      241 gcgagctgat cagctacgag tcgttcggcc cgccggcccg ccacggcgcg cgctacttct
      301 ggacccaccg cgacggcaag cagagccagc cggtggtgtt caccgccccc gggctcgacg
      361 ctgcgcccgg cgtgctgctc gatcccaacg cgatctcgac cgacggcagc ctcgcgttca
      421 tcgggctcgc cgtgaaccac gccggcaccc ggatcgcgta cggcctggcg cacggcggcg
      481 gcgactggca gacctggcgc gtccgcgacg tcgcgggcgg caaggacctg cccgacgagc
      541 tcgatcacat caagtactac cgccccgtgt tcacccacga cggcaccggc gtgttctaca
      601 gccggttccc cgcgccgccg cccggcaagg agctggtcga gaccgaccac gactgcaagc
      661 tctactatca ccggatcggc acgccggccg ccgccgacct cgtggtctac gagcgcccgg
      721 atcacccgac ctggcagttc gatccggtga tcaccgacga cggccgctac ctggtgatca
      781 ccaccggcga tggccaggtc ggcgatcgcg gcgtcgagct cgtcggctac gtcgatctcg
      841 agcgcccggg gcaaaaaccg gcgcccggca agcccgccgc gccgaccccg ctggtcgaca
      901 cctacgacgc cgagtatcgc ttcctgggca acgacggccc ggtgttctac ttccagacca
      961 cgctcggcgc cgccaacaag cggatcatcg cgatcgacac ccgccacccg gcgcgcgacc
     1021 gctggcagga ggtcgtcgcc gagggcccca acgcgatcca gaacgcgacc gtggtcggcc
     1081 gccagctgat cgtcaccacg ctccaggacg cccaccacgc ggtcgtcgcc tacgatctcc
     1141 acggcaagaa gctgcgcgac ctcgagctgc ccggcatcgg cagcgccaac ggcttcggcg
     1201 gcgaccccga cgcgcgcgag acgttctacc tgttcaacgg cttcaccgtg cccggcaccg
     1261 tctaccgcta cgagctggcg accggcaaga gcacgccgtg gaaggcgccg cacgtcgcgt
     1321 tcgactcggc gagcttcgag accacccagg tgttcttccc gagcaaggac ggcaccaagg
     1381 tgccgatgtt cctcaccgcg aagaagggcc tgccgcgcga cggcacgagc ccgacgatcc
     1441 tctacgcgta cggcttcggc ggcatctcgt cgacgccgcg gttcgatccg gcgcggatcg
     1501 cctggctcga gcgcggcggc atctgggcga tggtcaacat ccgcggcggc ggcgagtacg
     1561 gcgaggcctg gcacaacgcc gcgcgccgcg cccaccgcca ggtcgcctac gacgacttcc
     1621 tcgccgccgg cgactggctg gtcgcgaaca aggtcacctc gaccgcccac ctcggcgcga
     1681 ccggcagctc gggcggcggc atgttggtcg ccggcgcgct ggtgcagcgg cccgatctgt
     1741 tcgccgccgt gctcccgttc gccggcgtcc acgatctgct gcggttccac ctgttcggcc
     1801 agggcgcggg ctggcagggc gatctgggct cgatcgacga tccgaccgag ttcgccgcgc
     1861 tgcgcgcgac ctcaccgctc cacaacgtcc ggtccggcac ccggtatccg gcggtgctgc
     1921 tcatcaccgc cgatcacgat gtgcgagtcg cgccgctgca ctcctacaag ctcgccgctg
     1981 cgctccaggc cgcccaggcc ggccccgccc cggtcgtgct gcgggtcgag accgcatccg
     2041 gccatggcgg cggaaccacg cgcgcccagg cgatcgagca gcagaccgag cgctacatgt
     2101 tcttcgcggc caacctcggc ctgcgcgtgg attgatggat ggatccatag ggggctctcc
     2161 cgcccctccg cgcgtcgcgt ggcctcgcgt ccgccgggac cgggtatcgt cgtgccatgc
     2221 gtccctggat cctgctcctg gcgatcacga gctgctcggc gcccgcgatc gcgccgacgg
     2281 cgcccgcgcc gcccgccctc gctgcg
//
LOCUS       DASVOR010001945         3755 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488188,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001945 DASVOR010000000
VERSION     DASVOR010001945.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488188"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="VF516_36085"
     CDS             <1..242
                     /locus_tag="VF516_36085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113224.1"
                     /translation="TQGVRLMRVDDGEHVAAIERLAEPGETSGIAEGAPIEAVDDNDT
                     VPNELLDDEAEDSGDGGDGGDAGDDEDDAGDDPES"
     gene            complement(345..2399)
                     /locus_tag="VF516_36090"
     CDS             complement(345..2399)
                     /locus_tag="VF516_36090"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8113225.1"
                     /translation="MRSLVCAALLVACSSPSRPTAAPVPPSPPPPSPVSTAAPPTASP
                     APAAGANLEAKVMSGDTPATDSDGNTFIAPDGWKLATSGAMTVLISPEGDSYLALVAV
                     SAASSDAARDTAWKLYKPDARWPVLASNDVPDRDGWTKGKVYQYQTSPNEKRVVQAVA
                     RFAGGRWSVLLVDFTRATAEKRGGQIAKTFNRLYPKGYSGESFKGRTAARLDAARIEA
                     LKQFVRDAEQATGVPGVSFGVIQDGKTVWAGGIGVRELGKPAPVDDKTLYMIASNTKT
                     LTTLMLAKLVDAGKLVWDQPVTQVLPSFRLGDDALTRQVLVKHLICACNGLPRQDLEW
                     LMEWKGSTPESVLKTLGTMMPTSKFGELFQYSNLMAAAGGYTGGHAMYPNLELGAAYD
                     KAMQTLVFDPLGMTATTFDYAKALRGNHAMPHGIDIDGKPARDAMDENYSVISARPAG
                     AAWSNVDDLLKYLAMELAEGKLPGGKQYVSKDALLARRAPNVAIGTDAAYGMGLIIHT
                     KYDVTVYDHGGDLIGFHSDMMWIPDANAGAVVLTNGDLGPSIRTQFQRKLLEVLYDGK
                     PEADENVAQGAKSFFATLAAERKLLTVPADPTEAGKLVTSYKNASLGEIAVGHKAGKT
                     IFDFGEFRSEVATLRNPDGTISFETIEPGTQGFAFVAGEAAGKPTLTLRDGQHEYVFT
                     TR"
     gene            2662..>3755
                     /locus_tag="VF516_36095"
     CDS             2662..>3755
                     /locus_tag="VF516_36095"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8113226.1"
                     /translation="MNRLRSVVPCVAIAWFCAPRAAAADAPAACPGDYAEDLSALSPR
                     ARAIDAATASYSYAVRTQATYECVSYGSDGNLKRTRSTALAYGTAFGYRRDGNDTLLL
                     TNEHVADWPTVTDDDHPVDGVAAGCKRVADSLKIVDDDHDDYAADDIPLTRVIVDPAL
                     DVAVLRAHAKLEIIPWRIGRSAALAARNVVQVKGYPLGEFHATNIGKVVSPYDRDTQG
                     VRNHDDFIVDALLTSGGSGSPVFAVSCKTGEFELVGIFHARYVRASALNVVVAIDQVR
                     DLMTTLKRSPRPADRSPELDAAARGRLIEAVRADPDPPYFAVGPLVASLHVRGDGALV
                     FALFASDFPRTPGPLLAVEDLAAPDPAAFG"
BASE COUNT          517 a         1342 c         1328 g          568 t
ORIGIN      
        1 cgacccaggg cgtccggctg atgcgggtcg acgacggcga gcacgtcgcg gcgatcgagc
       61 ggctcgccga gccgggcgag acctcgggga tcgccgaggg cgcgccgatc gaggcggtcg
      121 acgacaacga cacggtgccc aacgagctgc tcgacgacga ggccgaggat agcggcgacg
      181 gcggcgatgg cggcgacgcc ggcgatgacg aggacgacgc cggcgacgat ccggagagct
      241 gagctggtcg ccgacgtgat cagtccgcgc gacgcgacgc gcggaggggt gaatggaccg
      301 gcttcgccgg tccagagggg acgggaacgt cccctgtgga tcaatcaccg ggtcgtgaac
      361 acgtactcgt gctggccgtc gcgcagggtc agcgtcggct tgcccgcggc ctcgccggcc
      421 acgaacgcga agccctgggt ccccggctcg atggtctcga acgagatcgt gccgtccggg
      481 ttgcgcaggg tcgcgacctc gctcctgaac tcaccgaagt cgaagatcgt cttgccggcc
      541 ttgtggccga ccgcgatctc gccgagcgac gcgttcttgt agctcgtcac gagcttgccg
      601 gcctcggtgg gatccgccgg cacggtcaag agcttgcgct cggcggcgag cgtcgcgaag
      661 aagctcttgg cgccctgcgc gacgttctcg tcggcctcgg gcttgccgtc gtagagcacc
      721 tcgagcagct tgcgctggaa ctgggtgcgg atcgacggac cgagatcgcc gttggtgagc
      781 acgaccgcgc cggcgttggc atcggggatc cacatcatgt cgctgtggaa cccgatcaga
      841 tcgcctccgt ggtcgtagac cgtgacgtcg tacttggtgt ggatgatcag ccccataccg
      901 taggccgcgt cggtgccgat cgcgacattc ggcgcgcgcc gggccagcag cgcgtccttc
      961 gagacgtact gcttgccgcc gggcagcttg ccctcggcga gctccatcgc gaggtacttg
     1021 agcaggtcgt cgacattgct ccacgccgcg cccgcgggcc gcgcggagat caccgagtag
     1081 ttctcgtcca tcgcgtcgcg ggccggcttg ccgtcgatgt cgatgccgtg cggcatcgcg
     1141 tggttgccgc gcagcgcctt cgcgtagtcg aacgtggtcg cggtcatgcc gagcgggtcg
     1201 aacaccagcg tctgcatcgc cttgtcgtag gcggcgccga gctccaggtt ggggtacatc
     1261 gcgtgcccgc cggtgtaccc gccggcggcc gccatcaggt tcgagtactg gaacagctcg
     1321 ccgaacttcg acgtcggcat catcgtgccc agcgtcttga gcaccgactc cggcgtcgag
     1381 cccttccact ccatcagcca ctccaggtcc tgccgcggca gcccgttgca cgcgcagatc
     1441 aggtgcttga ccagcacctg ccgggtcaac gcgtcatcgc cgagccggaa gctgggcagg
     1501 acctgtgtca ccggctggtc ccagacgagc tttccggcgt cgaccagctt ggcgagcatc
     1561 agcgtggtca gcgtcttggt gttcgacgcg atcatgtaga gcgtcttgtc gtcgaccggc
     1621 gccggcttgc cgagctcgcg cacgccgatg ccgccggccc acaccgtctt gccgtcctgg
     1681 atcacgccga acgacacgcc gggcacaccg gtggcctgct cggcgtcgcg cacgaactgc
     1741 ttgagcgcct cgatccgcgc ggcgtcgagc ctggccgcgg tccggccctt gaacgactcg
     1801 cccgagtagc ccttcgggta gaggcggttg aaggtctttg cgatctggcc gccgcgcttc
     1861 tcggcggtgg cccgggtgaa gtcgaccagc aggacggacc agcggcctcc ggcgaaccgg
     1921 gcgaccgcct gcacgacgcg cttctcgttg ggcgaggtct ggtactggta gaccttgccc
     1981 ttggtccagc cgtcgcggtc cggcacgtcg ttgctcgcga gcaccggcca ccgcgcgtcg
     2041 ggcttgtaca gcttccaggc cgtgtcgcgg gcggcatcgc tggacgcggc cgagacagcg
     2101 accagcgcga ggtacgagtc gccctcgggt gagatcagca cggtcatcgc gcccgaggtc
     2161 gcgagcttcc agccatcggg cgcgatgaac gtgttgccgt cgctgtccgt cgccggcgtg
     2221 tcgccgctca tcaccttcgc ctcgaggttc gccccggctg cgggcgccgg cgaggcggtc
     2281 ggtggagccg cggtggacac cggcgacggg ggcggcggcg acggcggcac cggtgccgcg
     2341 gtgggcctgg atggggagga gcacgcgacg agcagcgcgg cgcacacgag gcttcgcatg
     2401 cgcggacgtt agatcactcg cgggcgcctc gtcgacgcag ttcacccacg gtgcaccgcg
     2461 acatgaggcg agccgcggcg tgtcccggtg ggacaccggc gagaccgcgg agccatgccg
     2521 cagccgcggg tccgtaccat ggcgcgccac cgcccactgc gcgctccggc ataccccggt
     2581 aggacacggc cgaaccccgt ggatcggcgc gcgatccgcg tcggggatga acgcccggct
     2641 cgtccggccg tcctatccag catgaaccgc ctgcgctcgg tcgtcccttg cgtcgcgatc
     2701 gcctggttct gcgcaccccg ggccgcggcg gccgacgctc cggccgcctg cccgggcgac
     2761 tacgccgagg acctgtcggc gctgtcaccg cgcgcccggg cgatcgacgc cgcgacggcg
     2821 tcgtactcct acgcggtgcg gacgcaggcg acctacgagt gcgtgtcgta cggctccgac
     2881 ggcaacctca agcggacgcg gtcgaccgcg ctggcctacg gcaccgcgtt cggctaccgc
     2941 cgcgacggca acgacacgct gctgctcacc aacgagcacg tcgcggactg gccgacggtc
     3001 accgacgacg accacccggt cgacggcgtc gccgccgggt gcaagcgggt cgccgactcg
     3061 ctgaagatcg tcgacgacga ccacgacgac tacgccgccg acgacatccc gctgacccgc
     3121 gtcatcgtcg atcccgcgct cgacgtcgcg gtgctgcgcg cccacgccaa gctcgagatc
     3181 atcccgtggc ggatcggccg gagcgccgcg ctggccgcgc gcaacgtggt ccaggtcaag
     3241 ggctacccgc tcggcgagtt ccacgccacc aacatcggca aggtcgtgtc gccgtacgac
     3301 cgcgacaccc agggggtgcg caaccacgac gacttcatcg tcgacgccct gctgacctcg
     3361 ggcggctcgg ggtccccggt gttcgcggtg tcgtgcaaga ccggcgagtt cgagctggtc
     3421 gggatcttcc acgcgcgcta cgtccgggcg agcgcgctca acgtcgtggt cgcgatcgat
     3481 caggtgcgcg atctgatgac gacgctcaag cgctcgccgc gccccgccga tcgctcgccg
     3541 gagctcgacg ccgcggcgcg cggccgcctg atcgaagcgg tccgcgccga cccggatccg
     3601 ccgtacttcg cggtcggccc gctggtcgcg tcgctccacg tccgcggcga cggcgcgctg
     3661 gtgttcgcgc tgttcgcgag cgactttccc cggacgcccg gcccgctgct cgcggtcgag
     3721 gacctcgccg cgcccgatcc ggcggcgttc ggcag
//
LOCUS       DASVOR010001946         6285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_254751,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001946 DASVOR010000000
VERSION     DASVOR010001946.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6285
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254751"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VF516_36100"
     CDS             <1..360
                     /locus_tag="VF516_36100"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8113227.1"
                     /translation="GHEVAAERGCLRCHTTDGSPFIGPTWAHSFGRMRHTADGHDVLI
                     DEAYLTESMMDPRAVIAAGFAPVMPSYQGVLTPEEASAIVEYIRSLRDVQPARPVPLP
                     TGPIALPAPEGASGGNP"
     gene            360..2021
                     /locus_tag="VF516_36105"
     CDS             360..2021
                     /locus_tag="VF516_36105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8113228.1"
                     /translation="MADPWDAPPAAQPDPALAIEPDYLRAGRGIRSWLLTHDHKRIAL
                     MFLIATTLALTLGGVFALLLRTKLLHPGPWLIEPLTYNRLFTLHGVVMVFMFMIPAIP
                     AVFGNFVLPMMLGARDLAFPRINLASFYVYLAGAALALWGMIHGGADTGWTFYAPYST
                     TTPTDVAPIALGVFVLGVSSIMTGLNFIATTHMLRRRGLRWHDMPIFVWAIYATSIIQ
                     VIATPVLGLAVSLVAIDHALHLGIFDPALGGDPVLFEHLFWFYSHPAVYIMILPAMGV
                     IAEVVPTFAKKQPFSYKALAYSSLGIAFVGFLTWGHHLFVAGISQFDAAIFGVMSMFV
                     AIFSAIKVFTWIGTLYRGSITFNTPMLYFFAFVYLFAFGGMTGIAVATTSLDVHWHDT
                     YFVIAHFHFIMIGGTLTAFLAALHYWFPKMFGRMYPERWGLVSAALVFAGFNLTFVPQ
                     FLLGNAGMPRRYYAYPDRFHVLNVMSTLGAYLLGAALILTLVYLLVSLRWGKPAPANP
                     WGSRGFEWRSPSPPPVYNFVVPFDPAHGAYAYDRPPPAEVPAEEA"
     gene            2018..2668
                     /locus_tag="VF516_36110"
     CDS             2018..2668
                     /locus_tag="VF516_36110"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HEX8113229.1"
                     /translation="MTAVAQTAAGPLHVQYDSLERQHTTARLGMWVFLGSESLLFAGL
                     LALYAAYRFAYPVEFHAASAHANLVIGTVNTYVLLTSSLTVALAIHATRRGHRRRTVA
                     LLVATIALGLVFDVLKAVEYAGHLREGLAPGVYYAFGELPARGVALYVTLYYLLTGLH
                     ALHVTGGIAALIWLAVRAQRGAFTPESHVALELGGLYWHLVDLVWIFLWPLLYLIR"
     gene            2665..3003
                     /locus_tag="VF516_36115"
     CDS             2665..3003
                     /locus_tag="VF516_36115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEX8113230.1"
                     /translation="MIREADHEPGREPGALGYVAVWLALVALATATLFLSRTVTGGWG
                     LVVALVIASAKAVLVAAFFMHLAHGRPTYRIVFAVAIGFLVLLVIGVLADVGTRSIAS
                     TYVDELGRPL"
     gene            3266..6160
                     /locus_tag="VF516_36120"
     CDS             3266..6160
                     /locus_tag="VF516_36120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113231.1"
                     /translation="MPHATRFVLRGILVASGALLCAASCQSSPPAERQAAPAAVTSPS
                     FLEFESGHVRPIALSPDGTRLFAVNTPNGSLEIFNVGDSGLAFAARVPVGMEPVAVAA
                     RDSNEVWVVNHLSDSVSVVTLGGTPHVTRTLLVGDEPRDIVFAGASNLAFITTAHRGQ
                     QRTDPSIAAVPGAGDPQLTTPSVPRADVWVFDPANLGNTLGGTPVKILSFFTDTPRAL
                     AVSPDRNTVYVAGFKTGNQTTVVIEAMVCEGFHPDTPCVNANGSVSPGGSPGPATNFE
                     GKPAPHVALIVKFNNATGHWEDELHRVWDHAIRFRLPDTDVFAVDANALTQTAAYAHV
                     GTTLFNMVTNPATGELYVSNTDAHNEVRFEGPGQFGGHTVQGHLAETHVTIVSNGAGG
                     PPGGSVTPIHLNKHIDYTKLAGQPGFDPTAAQHSLATPLDMAVTRDGATLFVAAFGSS
                     KIGVFDTAALRANSFDPRQISAGYIPVSGGGPSGLALDESRGRMYVMTRFDNAVKVVD
                     LAARQEIAALPLPNPEPASVVQGRPMLYDATHNSGNGEAACASCHIFGDNDDLAWDLG
                     NPDDIVRKSPIPGNLTDPLAITAGKLLFGFRGQINGTGNADDFHPMKGPMTTQTLRGL
                     VNSGAMHWRGDRANGFFGEDAFDPNLSFDNFIVAFPGLIGAAAQPPAAQMQAFTDFQL
                     QVVEPPNPVRALDNSLTASQAAGKSFYFGARAADGLNVPILGGLITTFEGFNCNGCHE
                     VDPSKGEFGTSKRASFEGVIGQIFKIPHLRNMYTKVGMFGDPKVETFDAPDSGNTGDQ
                     IRGFGFTNDGTIDTMFRFFTVLVFHPQVTAGFPLINPDRTRRDVEQYVLAFDTDLAPI
                     VGQQVTLTSGNAAAVGPRIALFEQRAGASFTSKALGGTVKECELIARLAQAGATRGFL
                     FAPSTSSFAPAGGGPALSDSALRALAQTPGQEITFTCVPPGQGARLAAAQ"
BASE COUNT          876 a         2412 c         1968 g         1029 t
ORIGIN      
        1 ggccacgagg tcgccgccga gcgcggctgc ctgcgctgcc acaccaccga cggctcgccg
       61 ttcatcggcc cgacctgggc ccattcgttc ggccgcatgc gccacaccgc ggacggccac
      121 gatgtcctga tcgacgaggc ctacctgacc gagtcgatga tggacccgcg cgcggtgatc
      181 gccgccgggt ttgcgccggt gatgccgtcg taccagggcg tgctgacccc ggaggaggcg
      241 tccgcgatcg tcgagtacat ccgctcgctg cgcgacgtgc aacccgcgcg cccggtgccg
      301 ctgcccaccg ggccgatcgc gctgccggcc ccggaaggcg cgtccggagg caatccatga
      361 tggccgatcc gtgggatgcg ccgcccgccg cgcagcccga tccagcgctc gccatcgagc
      421 ccgactacct gcgcgccggg cgcggcatcc ggtcgtggct gctcacccac gaccacaagc
      481 ggatcgcgct gatgttcctg atcgcgacca ccctcgccct gacgctcggc ggcgtgttcg
      541 cgctgctctt gcgcaccaag ctgctccacc ccgggccgtg gctgatcgag ccgctgacct
      601 acaaccggct gttcaccctg cacggcgtgg tcatggtgtt catgttcatg atcccggcga
      661 tcccggcggt gttcggcaac ttcgtgctgc cgatgatgct cggcgccagg gacctcgcgt
      721 tcccgcggat caacctcgcc agcttctacg tctacctggc gggcgccgcg ctcgcgctgt
      781 gggggatgat ccacggcggc gccgacaccg gctggacgtt ctacgcgccg tactcgacca
      841 ccacgccgac cgacgtcgcg ccgatcgcgc tcggcgtgtt cgtgctcgga gtgtcgtcga
      901 tcatgaccgg cctcaacttc atcgcgacca cgcacatgct gcgccgccgc ggcctgcgct
      961 ggcacgacat gccgatcttc gtgtgggcga tctacgcgac cagcatcatc caggtgatcg
     1021 cgacgccggt gctcggcctc gcggtcagcc tggtcgcgat cgatcacgcg ctccacctcg
     1081 ggatcttcga tcccgcgctc ggcggcgatc cggtgctgtt cgagcacctg ttctggttct
     1141 actcccaccc ggcggtctac atcatgatcc tgccggcgat gggcgtgatc gccgaggtcg
     1201 tgccgacgtt cgccaagaag cagccgttct cgtacaaggc gctcgcctac tcgtcgctcg
     1261 gcatcgcgtt cgtcggcttt ttgacctggg gccatcacct gttcgtcgcc gggatctcgc
     1321 agttcgacgc cgcgatcttc ggcgtgatgt cgatgttcgt ggcgatcttc tcggcgatca
     1381 aggtgttcac ctggatcggc acgctgtacc ggggctcgat cacgttcaac accccgatgc
     1441 tgtacttctt cgcgttcgtc tacctgttcg cgttcggcgg catgaccggc atcgcggtcg
     1501 cgacgacctc gctcgacgtc cactggcacg atacctactt cgtgatcgcg cactttcact
     1561 tcatcatgat cggcggcacg ctgaccgcgt tcctcgccgc gctgcactac tggttcccca
     1621 agatgttcgg ccggatgtac cccgagcgct ggggcctggt ctcggcggcg ctggtgttcg
     1681 ccgggttcaa cctgacgttc gtcccgcagt tcctgctcgg caacgccggc atgccgcgcc
     1741 gctactacgc ctatcccgat cgcttccacg tgctcaatgt catgtcgacg ctcggcgcgt
     1801 acctgctcgg cgccgcgctg atcctgacgc tggtctacct gctggtatcg ctgcgctggg
     1861 gcaagcccgc cccggccaac ccgtggggct cgcgcggctt cgagtggcgc tcgccgtcgc
     1921 cgccgccggt ctacaacttc gtcgtgccgt tcgaccccgc gcacggggcc tacgcgtacg
     1981 accggccgcc tcccgccgag gtccccgcgg aggaggcatg accgccgtcg cccagaccgc
     2041 cgccgggccg cttcacgtgc aatacgactc gctggagcgc cagcacacca ccgcgcgact
     2101 cggcatgtgg gtgttcctcg gcagcgaatc gctgctgttc gccggcctgc tcgcgctgta
     2161 cgccgcctac cgcttcgcct acccggtcga gttccatgcc gcgtcggccc acgccaacct
     2221 ggtgatcggc acggtcaaca cctatgtcct cttgacctcg agcctcaccg tcgcgctcgc
     2281 gatccacgcc acccggcgcg gccaccgccg gcggaccgtc gcgctgctcg tcgccacgat
     2341 cgcgctcggc ctggtgttcg acgtgctcaa ggccgtcgag tacgccggcc acctgcgcga
     2401 gggcctcgcg cccggcgtct actacgcgtt cggcgagctg ccggcccgcg gcgtcgccct
     2461 ctatgtcacg ctgtattacc tgctcaccgg gctgcacgcg ctgcacgtga ccggcgggat
     2521 cgccgcgctg atctggctcg ctgtgcgcgc gcagcgcggc gccttcacgc ccgagtccca
     2581 cgtcgcgctc gagctcggcg gtctgtactg gcacctcgtc gacctggtct ggatcttcct
     2641 atggccgctg ctctacctga tccggtgatc cgcgaagccg atcacgaacc cggccgcgag
     2701 cccggtgcgc tgggctacgt cgcggtctgg ctcgccctcg tcgcgctggc gaccgcgacc
     2761 ctgttcctgt cgcgcacggt gaccggcggc tggggcctgg tcgtcgcgct cgtgatcgcg
     2821 tcggccaagg ccgtgctcgt cgccgcgttc ttcatgcacc tcgcgcacgg ccggcccacg
     2881 tatcgcatcg tgttcgcggt cgcgatcggc ttcctcgtcc tgctcgtgat cggcgtgctc
     2941 gcggacgtcg gcacccgctc gatcgcgagc acgtacgtcg acgagctcgg ccggccgctc
     3001 tagccggatc gcccgggcgt taggttcgcg tcaagccgcc gtcgaggatg cgcccggcgc
     3061 ggtatggctg gcatggtcct gaccccagaa ctcactgatc tgacccgcct cgccccgcag
     3121 atccgcggtc tcggcgttct cgctcctcga gttacgcccg gcagcccttc gcggcgcctc
     3181 gagctcgcga gatctgattt gtgatccgga tcatctcagg gggagctccg gggttcactc
     3241 ggcaattttc tttgcggagg tccacatgcc ccacgccacg cggttcgttc ttcgcgggat
     3301 cctcgttgcc agcggcgcgc tgctatgcgc tgcctcctgt cagtccagcc cgccggccga
     3361 gcgccaggcg gcgccggccg cggtgacctc gccgtcgttc ctcgagttcg agagcggcca
     3421 cgtccgaccg atcgcgctgt cgcccgacgg cacccggctg ttcgccgtca atacccccaa
     3481 tggctcgctg gagatcttca acgtcggcga cagcgggctg gcgttcgccg cgcgcgtccc
     3541 ggtcggcatg gagccggtcg cggtcgccgc gcgcgacagc aacgaggtct gggtcgtcaa
     3601 ccacctctcg gacagcgtga gcgtggtgac cctgggcggc acgccccacg tcacacgcac
     3661 cctgctggtc ggcgacgagc cgcgcgacat cgtgttcgcg ggagcctcga acctcgcgtt
     3721 catcaccacc gcgcaccgcg gccagcagcg caccgatccg tcgatcgcgg ccgtccccgg
     3781 cgccggcgat ccgcagctca ccacgccgtc ggtgccgcgc gccgacgtct gggtgttcga
     3841 tccggcgaac ctcggcaaca cgctcggcgg cacgccggtc aagatcttga gcttcttcac
     3901 cgacacgccg cgcgccctgg cggtcagccc cgatcgcaac acggtctacg tcgccgggtt
     3961 caagaccggc aaccagacca cggtcgtgat cgaggcgatg gtgtgcgagg gcttccaccc
     4021 cgacacgccg tgcgtcaacg ccaacggctc ggtgtcgccg ggcggcagcc cgggacctgc
     4081 caccaacttc gagggcaagc cggcgcccca cgtcgcgctg atcgtcaagt tcaacaacgc
     4141 caccggccac tgggaggacg agctgcaccg cgtgtgggat cacgcgatcc ggttccgcct
     4201 gcccgacacc gacgtgttcg cggtcgacgc caacgcgctg acccagaccg cggcctacgc
     4261 ccacgtcggc accacgctgt tcaacatggt caccaacccc gccaccggcg agctctacgt
     4321 gtccaacacc gacgcgcaca acgaggtccg gttcgagggc cccggccagt tcggcggcca
     4381 caccgtgcag ggccacctcg ccgagaccca cgtcaccatc gtctccaacg gcgccggcgg
     4441 cccccctggc ggctcggtca cgccgatcca cctcaacaag cacatcgatt acaccaagct
     4501 cgccggccag cccggcttcg atccgaccgc cgcgcagcac agcctggcga cgccgctcga
     4561 catggcggtc acccgcgacg gcgcgacgct gttcgtcgcc gcgttcggct cgagcaagat
     4621 cggcgtgttc gacaccgcgg cgctgcgcgc gaacagcttc gacccgcggc agatcagcgc
     4681 cggctacatc ccggtcagcg gcggcggccc gagcggcctg gcgctcgacg agtcgcgcgg
     4741 ccggatgtac gtcatgaccc ggttcgacaa cgcggtgaag gtggtcgacc tcgccgcgcg
     4801 ccaggagatc gccgcgctgc cgctgcccaa ccccgagccg gcgtcggtcg tgcaaggccg
     4861 gccgatgctg tacgacgcca cccacaactc cggcaacggc gaggccgcgt gcgccagctg
     4921 ccacatcttc ggcgacaacg acgacctcgc gtgggacctc ggcaaccccg acgacatcgt
     4981 ccgcaagagc ccgatcccgg gcaacctcac cgacccgctg gcgatcaccg ccggcaagct
     5041 cctgttcggc ttccgcggcc agatcaacgg cacgggcaac gccgacgact tccatccgat
     5101 gaagggcccg atgacgaccc agaccctgcg cggcctggtc aactccggcg cgatgcactg
     5161 gcgcggcgac cgcgccaacg ggttcttcgg cgaggacgcg ttcgatccca acctgtcctt
     5221 cgacaacttc atcgtcgcgt tccccggcct gatcggcgcc gccgcgcagc cgccggccgc
     5281 gcagatgcag gcgttcaccg acttccagct ccaggtcgtc gagccgccca acccggtgcg
     5341 cgcgctcgac aactcgctga ccgcctcgca ggccgccggc aagagcttct acttcggcgc
     5401 gcgcgcggcc gacggcctca acgtccccat cctcggcggc ctgatcacca ccttcgaggg
     5461 cttcaactgc aacggctgcc acgaggtcga tccgtccaag ggcgagttcg gcaccagcaa
     5521 gcgcgcctcg ttcgagggcg tcatcgggca gatcttcaag atcccccacc tgcgaaacat
     5581 gtacaccaag gtcggcatgt tcggagaccc gaaggtcgag accttcgatg cccccgacag
     5641 cggcaacacc ggcgaccaga tccgcggctt cggattcacc aacgacggca cgatcgacac
     5701 catgttccgg ttcttcaccg tcctggtgtt ccatccgcag gtcaccgccg gcttcccgct
     5761 gatcaacccc gatcgcacgc ggcgcgacgt cgagcagtac gtgctcgcgt tcgacaccga
     5821 cctggcgccg atcgtcggcc agcaggtgac cctcacctcc ggcaacgccg ccgccgtggg
     5881 cccgcggatc gcgctgttcg agcagcgcgc cggcgcatcg ttcacctcga aggccctcgg
     5941 cggcacggtc aaggagtgcg agctgatcgc ccggctcgcc caggccggcg cgacccgcgg
     6001 cttcctgttc gcccccagca cgagcagctt cgcgcccgcc ggcggcggcc ccgcgctctc
     6061 ggacagcgcc ttgcgcgcgc tcgcccagac gcccggtcag gagatcacat tcacctgcgt
     6121 accgcccggc cagggcgccc ggctcgccgc tgcgcagtag tcatccgatg aaccatgggt
     6181 ggaggtgacg cccgaagtcg tgccggcgcg gaccatggca aacaccggca cccacgtggg
     6241 cgccgggcag gtgccgatag caaaccggca cgcggcaaca tggtt
//
LOCUS       DASVOR010001947         2342 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488211,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001947 DASVOR010000000
VERSION     DASVOR010001947.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488211"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VF516_36125"
     CDS             <1..1344
                     /locus_tag="VF516_36125"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8113232.1"
                     /translation="YLLTISQDSLPAWRSPSSPVGFSPRVALRNFGLLHAFLSGQACP
                     GALTDATQSVFYAPFTRLPSTIPAATADRLRAQGRAAIEQAVIPAYAKVARFLAETYI
                     PGARKTLGASELPDGRALYRAAIREHLTRDMSPEEVRDLGLREVARITQEMEAIRREV
                     GFTGSLPEMFVHLRTAPEFYATTPEALLMRAAWIAKWIDAQLPRWFATLPRQTYGVEP
                     VPDAIAPFYTGGRYVPAGEGSGRAGTYWVNTYDLPSRPLYVLPALTLHEAVPGHHLQA
                     AVAAEQHGLPMFRRYAYFDVTGEGWGLYAEKLGEEMGIYTTPYERFGRLTYEMWRACR
                     LVVDTGIHAFGWSRDQARAYMRDHTALSEHEIETEVDRYIGWPGQALAYKVGELAILA
                     LRARAEQELGPRFDIRRFHDVILAGGSLPLDVLQDEVARFIAAEKAAPDRAATAP"
     gene            1579..1725
                     /locus_tag="VF516_36130"
     CDS             1579..1725
                     /locus_tag="VF516_36130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113233.1"
                     /translation="MYQIGQGGVLQTQTFLNQALGAAYTPVALDPAGQVYALNHGELT
                     VFGR"
     gene            1874..>2342
                     /locus_tag="VF516_36135"
     CDS             1874..>2342
                     /locus_tag="VF516_36135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113234.1"
                     /translation="MSLSFAGCSTGVDCFDQGPLQAAYKTGGTDAVTANTAIYDRARA
                     IGLGLTHADGERDGTSEGYAAGHKDGVAASYPGGYSDGAIEGRAVGLSDPSACSTGAA
                     DGHAAGSSDGYSDGFSAGSSAGHDAGYSDGFDAGSSTCSGGDGGGSDGGGSDGS"
BASE COUNT          344 a          858 c          801 g          339 t
ORIGIN      
        1 tacctcctta cgatctcgca agactcgctt ccggcgtggc ggtctccgtc gtcgccggtg
       61 ggattttcac cccgggtcgc tctccgaaat ttcgggctgc tacatgcctt cctttccggc
      121 caggcttgtc ctggcgccct caccgacgcg acccagagcg tgttctacgc gccgttcacc
      181 aggctgccga gcacgatccc cgccgccacg gccgaccggc tgcgcgcgca gggccgcgcg
      241 gcgatcgagc aggcggtgat cccggcctac gcgaaggtcg cgcggttcct cgccgagacc
      301 tacatccccg gcgcgcgcaa gaccctcggc gcgagcgagc tgcccgacgg gcgcgccctg
      361 taccgcgccg cgatccgcga acacctcacc cgggacatgt cgcccgagga ggtccgcgat
      421 ctcggcctgc gcgaggtcgc gcgcatcacc caggagatgg aggcgatccg gcgcgaggtc
      481 ggattcaccg gcagcctccc cgagatgttc gttcacctcc gcacggcgcc cgagttctac
      541 gcgacgacgc ccgaggcgct gctgatgcgc gcggcgtgga tcgccaagtg gatcgacgcc
      601 cagctgccgc gctggttcgc gacgctgccg cgccagacct acggcgtcga gccggtgccc
      661 gatgcgatcg cgccgttcta caccggcggc cgctacgtcc ccgccgggga gggcagcggc
      721 cgcgcaggca cctactgggt caatacctat gatctcccga gccggccgct ctacgtgctg
      781 cccgcgctca cactgcacga ggcggtcccc ggccaccacc tgcaggccgc cgtcgccgcc
      841 gagcagcacg gcctgccgat gtttcgccgc tacgcctact tcgacgtcac cggcgagggc
      901 tggggcctgt acgccgagaa gctcggcgag gagatgggca tctacaccac gccctacgag
      961 cggttcggcc ggctcaccta cgagatgtgg agggcgtgcc ggctggtcgt cgataccggc
     1021 atccatgcgt tcggctggtc gcgcgaccag gcgcgggcct acatgcgcga tcacaccgcg
     1081 ctgtccgagc acgagatcga gaccgaggtc gaccgctaca tcggctggcc cggacaggcc
     1141 ctggcctaca aggtcggcga gctcgcgatc ctcgcgctgc gcgcacgcgc cgagcaggag
     1201 ctcgggccgc gcttcgacat tcgccggttc cacgacgtca tcctcgccgg aggttcgctg
     1261 ccgctcgacg tcctccagga cgaggtcgcg cgcttcatcg ccgcggagaa ggccgcaccg
     1321 gatcgcgcgg cgacggcgcc ctgacggcga gcgcggacca gccgagacga cgctcggctt
     1381 cgatcctgtc gcaggcggtt cgcgcgcccg tccgggtcca gtacacgcgg caggacgaga
     1441 tgaagtggga gaactacggc gcggcgtgtg cgatcgagct gcgcgctgcg ctcggcccgg
     1501 acggcacgat cgccgcatgg gatcgcgagg cctgggtcgc gtcgctcggc agccgcccgg
     1561 ggtaccgagg acggctacgt gtaccagatc ggtcagggcg gtgtgctgca gacccagacg
     1621 ttcctcaacc aggcgctcgg cgcggcgtac acgccggtcg ccctcgatcc ggccggccaa
     1681 gtctacgcgc tcaaccatgg cgagctcacg gtgttcggcc ggtgaccatg taaagaagcg
     1741 tgaactgcgg aagccgcagg actgacgcaa cgacgccaca cgatcccgac ggggcaccgc
     1801 gggcttggcc cgatcagatc gggacttcgc tttgcactga gagcttcacg tgcgtcttct
     1861 ccttgcctgc atcgtgagtc tctcgttcgc cgggtgctcg accggggtcg attgtttcga
     1921 tcagggtcca ctccaggccg cctacaagac cggcggtacc gacgcggtca cggccaacac
     1981 cgccatctat gaccgcgccc gcgccatcgg actcggcttg acccacgccg acggcgagcg
     2041 cgacggcacg tcggagggct atgcggcagg tcacaaggac ggcgtcgccg ccagctaccc
     2101 gggcggatac tccgacgggg cgatcgaggg ccgcgccgtc ggtctgtccg atccgagcgc
     2161 ctgctcgacc ggcgccgccg atggccacgc ggctggatcc tccgacggct acagcgacgg
     2221 cttctcggcg ggctcgtccg ccgggcacga cgccggctac agcgacggct tcgacgctgg
     2281 ctccagcacg tgcagcggcg gcgatggcgg cggcagcgac ggcggcggca gcgacggcag
     2341 cg
//
LOCUS       DASVOR010001948         2068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488243,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001948 DASVOR010000000
VERSION     DASVOR010001948.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488243"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1652)
                     /locus_tag="VF516_36140"
     CDS             complement(<1..1652)
                     /locus_tag="VF516_36140"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8113235.1"
                     /translation="MTPGDLFGDRFEIEQSVDSGGMGTVFRARDPISGEAVAVKVIAD
                     ERSHVAERFAREVQVLAELSHPGIVRYISHGVTPSGELFLAMEWIDGEILETRLQRGP
                     LTISDAVTLATRVADALGAAHALGIVHRDLKPSNLILPGGRIDQVKVLDFGIARRAQG
                     TQLTRTGMMLGTPGYMAPEQARAGGQIDARADVFALGCVLFECLTGVPPFDGDTTAAV
                     LARILFDAAPRVSELWPEVPESLDALVAQMLAKDPALRPGDGASLAAALAALGPMANT
                     APVAPRERASRARSTTTRERRLLAVVMLGPAAQDDAWLDDSLQRAVAPHGGRLEQLAD
                     GSTVVVLDADHQVATDQAARAARSALAMSALAGHRPLAIAMGRTESGSRLPEADVIDR
                     VSRLLAHVGGRPGELPAIALDEVSAGLLDARFDVIEHESQLRLRGERAVMQGARTLLG
                     RPTSCVGRDWELGAFAGMLDECIEEPAARVALVTAAAGMGKSRLGAEFISRVRERLPE
                     VAVWIGRGDSLRAGSTLDLLAQALRGALDLRDGEPLSVRHDKL"
BASE COUNT          281 a          776 c          739 g          272 t
ORIGIN      
        1 agcttgtcgt ggcgcacgga cagcggctcg ccgtcgcgga ggtcgagcgc gccgcgcagc
       61 gcctgggcca gcaggtcgag cgtcgagccg gcgcgcagcg agtcgccgcg gccgatccag
      121 accgcgacct cgggaaggcg ctcgcggacc cggctgatga actcggcgcc gagccgcgac
      181 ttgcccatcc cggccgccgc ggtcaccagg gcgacgcgtg cggcaggctc ctcgatgcac
      241 tcgtcgagca tgccggcgaa cgcgccgagc tcccagtcgc gtcccacgca cgaggtcggc
      301 cggccgagca gcgtccgcgc gccctgcatc accgcgcgct cgccgcgcag ccggagctgg
      361 gactcatgct cgatcacgtc gaaccgcgcg tcgagcagac cggcgctgac ctcgtcgagc
      421 gcgatcgccg gcagctcacc cggccgcccg cccacgtgtg cgagcagccg ggacacccgg
      481 tcgatcacgt cggcctcggg cagccggctc cccgactcgg tgcggcccat cgcgatcgcc
      541 agcgggcgat gcccggcgag cgcagacatc gcgagcgcgc tgcgcgcggc ccgggcggcc
      601 tgatcggtcg cgacctggtg gtcggcgtcg agcaccacga ccgtcgagcc gtcggcgagc
      661 tgctcgaggc ggccgccgtg gggtgcgacc gcccgctgca gcgagtcgtc gagccacgcg
      721 tcgtcctggg cggcggggcc cagcatcacc acggccagga gccgccgctc cctggtggtc
      781 gtcgagcgag ctctcgatgc gcgttcccgc ggcgccacgg gcgcggtgtt cgccatggga
      841 ccgagcgcgg ccagcgccgc cgccaggctc gcgccgtcgc cggggcggag cgcggggtcc
      901 ttcgccagca tctgcgcgac cagcgcatcg aggctctcgg gcacctcggg ccacagctca
      961 ctcacgcggg gtgcggcatc gaacaggatc ctggccagga cggcagccgt ggtgtcgccg
     1021 tcgaacggcg ggacgccggt caggcactcg aacagcacgc agccgagcgc gaacacgtcg
     1081 gcgcgcgcgt cgatctggcc gccggcgcgg gcctgctcgg gcgccatgta gccgggggta
     1141 ccgagcatca tgccggtccg cgtgagctgg gtgccctgcg ctcgccgcgc gatcccgaag
     1201 tcgagcacct tgacctgatc gatgcgcccg ccggggagga tcaggttgga cggcttgagg
     1261 tcgcggtgga cgatgccgag cgcgtgcgcg gcgccgagcg cgtcggcgac ccgggtcgcg
     1321 agggtgaccg cgtcgctgat ggtcagcggc ccgcgctgga ggcgggtctc gaggatctcg
     1381 ccgtcgatcc actccatggc caggaacagc tcgcccgagg gcgtcacgcc gtgcgagatg
     1441 tagcggacga tgccgggatg ggagagctcc gcgagcacct ggacctcgcg cgcgaagcgc
     1501 tcggcgacgt ggcttcgttc gtcggcgatg accttgacgg cgaccgcctc cccggagatc
     1561 ggatcgcgcg cgcggaacac cgttcccatg ccgccgctgt cgaccgattg ctcgatctcg
     1621 aaccggtcgc cgaacaggtc tccgggtgtc atgaaggaat acccgtggca accatagtcc
     1681 gcacgagacc gaggcgcaga ccctcgcgcc gttccccgtc tactttcacg gaacactcac
     1741 tttccaaaaa accggggcgc cgaacttgcc tcgccagacc atcggcgggc atggccgcga
     1801 cccgcggtgg tgccagcgcc tcgctcccgc gctgccatgg acacggctcg cggtcctccg
     1861 ctcaccccga ccacgacaaa tccttgtaag acttcggtcg agctccggac tacctgacat
     1921 ggcgatcgca gcggtcgtcc aggaggcccg tgtgagctcg tccccggatc ggtttccccc
     1981 agcgttcttg ctcgcctgcg cgctcgtcgc ctgcgcgcat cccggcccgg ctccggcccc
     2041 ggccgtggcc cccgcggcaa cggcgccg
//
LOCUS       DASVOR010001949        17659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_21676,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001949 DASVOR010000000
VERSION     DASVOR010001949.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 17659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17659
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21676"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VF516_36145"
     CDS             complement(<1..342)
                     /locus_tag="VF516_36145"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEX8113236.1"
                     /translation="MSPLPHRVRRLSFQVRATSSGEALALRAALHQQIDTVTAAMTRA
                     LDAAGAGDAIVHLPRLEVRARIASVDEVGDALAQRIEHALRRELSARPGRSPGTDIAD
                     TGAVDARGGTVE"
     gene            complement(346..807)
                     /locus_tag="VF516_36150"
     CDS             complement(346..807)
                     /locus_tag="VF516_36150"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX8113237.1"
                     /translation="MAASAPRDTYSSFLGTGWSFPPEFTDGGRDVVMLSDEDDIRASL
                     TILFGTVRGERFMIPQYGLDPQELLFEPFSTTMRAFLKDRISTAILIQEPRIRVLALD
                     VAMVDPDAGVLQISLDYEVRATNSRYNLVYPFYRHDSSELLGTISGGRPPG"
     gene            complement(826..1104)
                     /locus_tag="VF516_36155"
     CDS             complement(826..1104)
                     /locus_tag="VF516_36155"
                     /inference="COORDINATES: protein motif:HMM:NF017315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEX8113238.1"
                     /translation="MPPAARVTDTTTHGGTITGPGVSTVLIGNLPAAVAGDMHVCSLP
                     PSSHPLTVSAFPSGSTSVLIQGRPALRTSDTCLCGAAAAVGEPTVLIG"
     gene            complement(1115..2857)
                     /gene="vgrG"
                     /locus_tag="VF516_36160"
     CDS             complement(1115..2857)
                     /gene="vgrG"
                     /locus_tag="VF516_36160"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEX8113239.1"
                     /translation="MSDERTLPIPASHREVTVKVDGEEVPREHPLLAMSVIKSVNRIS
                     SARLAYQDGSAATGDFPLSNADRFVPGKEIEILAGAGDDKTSLFRGVIVRQSIKVRDQ
                     RPTQLVVECRHKAQRLTVGRKAAYYLDKTDSDILSSLLSGAGVDADVEATQVSHKQQV
                     QLAASDWDFLLARAEANGKLVFTNDAKVAVKAPSFSGSAVCTLQFGATLLELDAQIDA
                     RLQFSAVKTRTWDPAQQAVVEKSAADPGVSGPGNLTSDDLSAVAGLDSYDLRHASLTE
                     DEAQAWADAAWLKSKMSKVSGVAKCEGIGTVNPGDLVTLAGVGDRYNGDVLVTGVRHE
                     FDLVQGWKTHVQFGGTEAWAAQERDGSAPRAGALVPAASGLAIGKVVSNEDGDGEHRV
                     RVRLPLVGMEDDGIWARVASLDAGDQRGFFFRPELDDEVVVGFLDDDPRRPVILGMLH
                     SSAKAAPLTGSNDNHEKVYQSRSRMRLYFNDDKKVMVFDTPAGNKITLSEDDKAVKIE
                     DQNGNKIEMTDSGIKIEAAKALQLKAGTEIKLESGTSFDVKGGTELKMEGSSGVEVTS
                     SAVTKIKGSLIQLN"
     gene            complement(2860..3540)
                     /locus_tag="VF516_36165"
     CDS             complement(2860..3540)
                     /locus_tag="VF516_36165"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113240.1"
                     /translation="MAGTGNDSGELERLVIRAYEQADYSGDAIDEFEAYVNPTEITLA
                     YEIEYDQAQGAGTTNSRMSFKKVKPGDLSLTLFLDGTGANGRPCDVQDKIEQFQTVTG
                     YNGNIHRPNYLKIGWGKLTIKRCVLKSASIAYKLFKSDGVPLRAVITASFSDNSDDQT
                     RVAMAQDESPDLTHFRVVKAGDSLPSLCTEIYGDPHLYLRVAQANQLDDFRTLVPGMR
                     LRFPPLEN"
     gene            complement(3544..3711)
                     /locus_tag="VF516_36170"
     CDS             complement(3544..3711)
                     /locus_tag="VF516_36170"
                     /inference="COORDINATES: protein motif:HMM:NF039278.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HEX8113241.1"
                     /translation="MPIFVDEVVISVDVDNPAAGGARPGSGSSGSEDKQALVAECVER
                     VLEILAQKQER"
     gene            complement(3715..4161)
                     /locus_tag="VF516_36175"
     CDS             complement(3715..4161)
                     /locus_tag="VF516_36175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8113242.1"
                     /translation="MPSYYPPVGFHFKVEFVGLPPNENDVRFTEVGGLSYEMVTEELA
                     EGGEQRFVQKYPVRTKYPELTLKRGLLVSSEIIGWVRRCIEDYQIEPQNLDVKLLNDE
                     HQPLLTWHVVGAYPTKWAVSDLNAANNAVVIETLQLYYQYFTMDRS"
     gene            complement(4161..4595)
                     /locus_tag="VF516_36180"
     CDS             complement(4161..4595)
                     /locus_tag="VF516_36180"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8113243.1"
                     /translation="MPTQYPIPVFHFTVQWGGDRVGFSEVSGLTQENQAIEYRDGSFP
                     EYSSIKMPGLRKFSNVTLKRGIVKGDNQFFKWLSTVKLNTVERRDLIVNLLNEEHQPV
                     MVWKVMRAFPVKVEGPQLKASGNEVAIESIELAHEGLELQNE"
     gene            complement(4652..6112)
                     /locus_tag="VF516_36185"
     CDS             complement(4652..6112)
                     /locus_tag="VF516_36185"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX8113244.1"
                     /translation="MATYRAPDVYIEEISTFPPSVAQVGTAIPAFIGYTQQATQTVAG
                     DLKGEPTKIYSLKAFEAYFGFPKAEDIKVSVDVDGDGKPIAGNVTPPGVSYFMYYAVK
                     LYFDNGGDQCYIVSVGDYTGAIDKAKLIDGLAAIGLEDEPTLLIVPEAARLGYADYKD
                     LCGAMLQQCSTLQDRFAILDVWKGSEKDDASVETVAGTKVNVIQANRDAWAGDLKYGA
                     AYYPFITTTMNHYVRDDEGNVKVTTNPPAGGGGGGGGGGGGGAGGGAPAADDLGKIRT
                     TKPAVYNFVKTKLKDWFVDTPPSGAIAGVYAATDAARGVWKAPANVALLGVLAPVVKL
                     NRLKQETLNVDPNTGKSINAIRAFAGKGTVVWGARTLLGQDNEWRYISVRRFFNMVEE
                     SVKKSTYWAVFESNDANTWVKVRGMIENYLIDKWREGALAGATPKDAFFVKCGLGTTM
                     SAQDILEGRMNVEIGMAVVRPAEFIVLKFSHKLQTS"
     gene            complement(6124..6939)
                     /locus_tag="VF516_36190"
     CDS             complement(6124..6939)
                     /locus_tag="VF516_36190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113245.1"
                     /translation="MTRFKQLFSLEVWHDYYGGVCPDVGFVIPDEAASTLRGAGMFAK
                     VVGGTLYVVFRSDDAGAPVQSVAGKTLRFGLVIRDPYFANITDGHDPSSGALHYRNHQ
                     VATALDDPPAHVVLRDVAPALWRDGALGLVDITLDPGFYTSAASFQIRFKARLDTWRY
                     YVVVKGFSNGDVDQLAVQPQTSGAPGPGDVTFQKVAPDQLTAEEKSRSDVLATDGAKV
                     LLFRSVTGIPRRETGAKQIQLMRNTEAVIERLPQPGKRRGTADLIVQLSKSKP"
     gene            complement(6939..7520)
                     /locus_tag="VF516_36195"
     CDS             complement(6939..7520)
                     /locus_tag="VF516_36195"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX8113246.1"
                     /translation="MLDVALNFLTTELNAYLVARGARKSTDEIGKAEVGKLVDDAGKW
                     VISDDHIGVSLIHIEEDRVMKSQLPEAILARGRQVILEPALKLNLHILFAAKFQKYDE
                     GLRSLSLVLTYFQSHPTFTPDTYSGLDPRIERLTVELQPLSYEQLNQIWTFVGAKQLP
                     SVIYKVRMVALQDLEPTAVAPPVSEIQTAATAT"
     gene            7727..7912
                     /locus_tag="VF516_36200"
     CDS             7727..7912
                     /locus_tag="VF516_36200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113247.1"
                     /translation="MKEINFTVTIDEANLILKGIGLLPFADVYQLVAKLQQQAGRQIE
                     RDTGNGEDRRPVSVQGT"
     gene            7989..12380
                     /locus_tag="VF516_36205"
     CDS             7989..12380
                     /locus_tag="VF516_36205"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX8113248.1"
                     /translation="MALARDFPDPPSSERDGTSQAGRAQPALDPAYVAVDERSARDLL
                     VLAHAYTRQLNFYDAQDRLAGDWTGFLGNDDLEALAALVDDSGPPGPSAVRMDRPHVA
                     LFLAFLRLLRHPRDLVNTLTARHLTFYYTQLLQMSRTSAVGDRVHVLVKLATGVDQAR
                     LPQGTALRAGKDSLGREQIYRTDRELVVSRAKLARLSSVYAQKEIVGLREAREHARGT
                     REEAFLAMLRIALGAPRPGDPLPQYGGKDVTYPLLLELKQRVDFAAASLFMDFSDLHA
                     MMQLVRRRAADAGDWATINAILEKAGQTRLHDPTWKLNPANPRDFSANLAKALGGAPD
                     FQRDGLSGVNSLADLYYQLFRADVIAYVHDKLFLSEADFRAMMGVKIPIDAQWGEIDR
                     ILEQAGRRRWNNPTYRLHPADPTAFAANLQAAIGPVPFSSLPHAILPAIADIGAYYDA
                     ILALEAYFHMNAAQLGYVLATAEKPAPSATPREWDTVYRILSDAHRDLVNAQRDQLDA
                     DRRQALQKAREAAPNAVQGFLAVLRIALGMGAASQPSLADLRPDVQKPEDYSFLESVQ
                     SRLVSGTITPAEWSRVYQVVVLAQRVRDQLPDPVAQRETWLDLRAADDATAIAAGPAE
                     LPRWRTFGQTAPVTSTDAPHPALTALIGWAISSPLLVVTQGTRTLTLTLGVPAKDFGN
                     RGIVRLLPPYAPLDQLPFELQISTEKGWLPLTVSAVVGDYRTLSQVSRAVPDAPQAIQ
                     LTATLADDAAPAAPNQTTAWAGGYPVLRLLLRRIWDASRGQYISLYEPFRDLPLVAAH
                     LRVSVTGLTPSSIQNDDAVLNARKPFEPFGSRPAVGSRFYVADPELVIKRLDGVAFHI
                     DWMGVPADLAQHYANYDVAGQAFSGTFTAQVSLIDNHLRMPLSTTPSSLFASGDPTKR
                     QDLAFAGLPTMLERAGYHYAPSSLPPGGPEVTAWPRYFEWELTPTDFKHQAYPAAAAQ
                     RALELTAALIKTPATANPASYQVKPPYTPKIKALRIDYAAQTEVVLDAAQPAAGTDRL
                     FHIHPFGASPIAAEADATGTPLLPRYEHEGELYLGIQNARPPETLSLLFEMADGSANP
                     DLPPVNIEWSYLSGDAWVTLQDGHVLLDTTNGLIDSGIVQIALPPADPSTRLPGDLYW
                     IRAAIANHPDSVCDTVGIHTQAVSATFDDHGNAPDHHAHALPARSIAGLAQRVPQIAG
                     VEQPYPSRGGRAAEAERGFFTRISERLRHKQRALTPWDYERLVLERFPGIGKVKCLPA
                     DPDNLGAVQIIVIPQIQGQSLANRFEPKVSAAQIADITAYLAGHSPPHAALHVRNARF
                     VQVKVRVGVRFQGTGDDGFYRSLLDDELVRYLSPWAFDDGADIAIGGKIFANSIVSFV
                     DGRDYVDYVANIELFSSNDGGRTFQLAVPQPSQGYFVSTDQPDAVLVTAPDHDIDIIP
                     DTGYTAVSFTGINYMKIELDFIVAGSA"
     gene            12414..14168
                     /locus_tag="VF516_36210"
     CDS             12414..14168
                     /locus_tag="VF516_36210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113249.1"
                     /translation="MPQLTTRPRGDLKTFFAKNAIPTESNFADLIDGMLNQQDDGIAK
                     LPNDALMIVAAGDATSQKKALHFYYAAADTDPSWTLSLNPRQNPAAANTANLGFSISD
                     GAGNSRLFIERSTGRIGVGTVGPLQAALHIDRGATNDLALLLSSSGPGWGSGLQLKNT
                     AAGAAHTYGIYTGGGTLHFADVDASADRMAILGDGTVWVPGKVTIGVSTPVSDLTLRR
                     DATAALGPIITLYNMAGAAGAGGAIDFNGYNVGANPPTARIQSLDDGIFSSHVVISTK
                     NSGAVANPLVERMRISSGGIVSFTGAIMPSAGNSSVRGIQFPSNPGGGGGDEAFIRYY
                     PVSGETCKLLIGINNDADDTIGLYQAGAERLTVGYGYVDVVADGNPIRFTSFWTGFAD
                     SATNRAEISNDTGGYKTLMIVGNKSAGHERRVGVWDRLDVNGSFWVNGAQKFDLAEVT
                     PARADDQLEQGDVVVIDREDGLRVRRSARPHDPAVYGVVSSYAQAAMVIGGSGDPDEM
                     TQAPDRRPIALVGRVKIKVCSETGPIRAGDLLTTSSRPGHAMRCADPAAHPGAIAGKA
                     LEPFSGDTGTITALVTLQ"
     gene            14173..>17659
                     /locus_tag="VF516_36215"
     CDS             14173..>17659
                     /locus_tag="VF516_36215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113250.1"
                     /translation="MSDPTSDTAELSTAPFEHPALDYSLLRSEGIQHLERLIGHVWND
                     FNPHDPGITILEQLCYTITDLAYRASYDVPDLLASSGGDPYRSLYRPSEILTSHPVTL
                     LDLRKLIIDVDGVHNAWIEPIAEPAPALYYRDDKREIGFRADPPYTVPVGRQGRFRAL
                     IETSDLVDRGSDVQRDVARRLSTCRPLCTDFELQVLVNQPIQVFAEVEIGPVDDAASV
                     LLAIYERLTEQISPSAPFQTLGEMLAAGKRSDEIFDGPLLDHGFLDAGALAGMTRKDA
                     IHASDLIREIMRLPEVKAVRSIALAKGTAAREPWSLSLDPTQTPSFDPRGSSIVLVKG
                     QLTAKIDTQRVIDTLLERRRQAASRSRLSPEDRDFALPAGRDRNIALYRSIQHQFPAL
                     YGIGTAGLPASVSDRRRAQARQLKAYLLVFDQLLATSLAQLAHAGSLFSFHADDPRTY
                     FTQPVEDDALDLSEIRTQDTGDDLAQLDDPAGSPSSRRNRFLNHLSARFAEQFTDYSL
                     VAGQAPGSDGDRGVRDKQAFLQHYPRISSARGTGMDAASAASETDLSGLQERIERKLG
                     LSAADGERLLVVEHLLLAPIAEDNVPPGKLDYAQTPILSGAVARDPYSLQLSVIASWR
                     GRLIGARDTADELRVLVEHTVREETPAHLTPYLHWPDETRWPAFDRAYQEWRDAYRGY
                     SAAKLDLALADDPRYFRMRDARDRMIDLLGFGKTYPLRDLPVRDERLTVPFNQPAGIP
                     IDASQRGVTYVLRNDQGDKDPGPAPITADGTGGTIYLETPPMRDDTTFTILARKLSPT
                     REVFLLHQPVVKVGLDVTLRARTRADPLDPSIEQPADDDARIVAWGTRATVQIDHSQE
                     GVDYHLVQITGTQEKPVSPDVRGNLGNIALTADAATEDVDLRIRATKVFDPSEHRDTQ
                     TALLAIVLPLRVRARLDLPVAPEPSALIDFRATPSVRIDSTQASASYALYVRAVSDRD
                     YVFSAPVPGLLAADVDGAPRVYVTRPPQRPIWQDGDGFQPAGAAVQGTGGTLHLPLPA
                     LRDDSVIAVRAHKDHRQGAATLPSSVQLAQAALILVRPDPQPAIQLAVGIDGGKTTGT
                     VDVTGSQPGVYYELRRDTDGPLLGLPGYFHKTDEQAPAANKGIGADPAYGLRIGVDLA
                     ISRGTQRPAATRDELA"
BASE COUNT         2761 a         6310 c         5827 g         2761 t
ORIGIN      
        1 ctcgacggtc cccccgcgcg catcgaccgc cccggtgtcc gcgatgtccg taccgggcga
       61 acggccgggc cgcgcgctca gctcgcggcg cagcgcgtgc tcgatgcgct gtgcgagggc
      121 atcgccgacc tcgtccaccg acgcgatccg cgcacggacc tcgagcctcg ggaggtggac
      181 gatcgcatcg ccggcgcccg ccgcgtcgag cgcacgcgtc atcgcggcgg tcaccgtgtc
      241 gatctgctgg tgcagcgccg cgcgcagcgc cagggcctcc ccggacgagg tcgctcgcac
      301 ctggaacgac agccggcgga cgcgatgcgg cagcggcgac atgggctagc ccggcggacg
      361 gccacccgag atcgtcccga gcagctcgct gctgtcgtgg cggtagaacg gatagaccag
      421 gttgtatcgg gagttggtcg cgcggacttc gtagtccagc gagatctgga gtacgccggc
      481 atcgggatcg accatggcga cgtcgagcgc caggacgcgg atccgcggct cctggatcag
      541 gatcgccgtc gagatccggt ccttgaggaa ggcacgcatc gtggtcgaga acggctcgaa
      601 caggagctcc tgcggatcca gcccgtattg cgggatcatg aaccgctcgc cacgcaccgt
      661 gccgaacagg atcgtgaggc tcgcgcggat gtcgtcctcg tcggacagca tcacgacgtc
      721 gcggccgccg tcggtgaact ccggcgggaa gctccacccc gtgccgagaa acgagctgta
      781 ggtgtctctg ggtgcgctgg ccgccatgcg tggctcctgt cggcgtcagc cgatcaacac
      841 cgtgggttcg ccgaccgccg ccgcggcgcc gcacagacag gtgtccgagg tgcgcagcgc
      901 cgggcgcccc tggatcaaga ccgacgtgct gcccgacggg aacgcgctca ccgtgagcgg
      961 atgactgctg ggcggcagcg agcagacatg catgtcgccg gccaccgccg ccggcagatt
     1021 gccgatcagc acggtcgaga ccccgggacc ggtgatggtc ccgccgtggg tggtcgtgtc
     1081 ggtgacgcgt gctgcgggtg gcatcgctgg ttcctcagtt gagctggatg agactgccct
     1141 tgatcttggt caccgccgag ctcgtgacct cgacgccgct cgacccttcc atcttgagct
     1201 cggtgccgcc cttgacatcg aacgacgtcc cggactcgag cttgatctcg gtcccggcct
     1261 tgagctgcag cgccttcgcg gcctcgatct tgatcccgga gtcggtcatc tcgatcttgt
     1321 tgccgttctg gtcctcgatc ttgaccgcct tgtcgtcctc gctgagcgtg atcttgttgc
     1381 cggccggcgt gtcgaacacc atgaccttct tgtcgtcgtt gaagtacagc cgcatcctgg
     1441 accggctctg atagaccttc tcgtggttgt cgttcgagcc ggtcaacggc gcggccttgg
     1501 cgctgctgtg cagcatgccg aggatcaccg ggcgccgcgg atcgtcgtcg aggaaaccga
     1561 ccacgacctc gtcgtcgagc tcggggcgaa agaagaagcc gcgctgatcg ccggcgtcga
     1621 ggctggcgac ccgggcccag atcccgtcgt cctccatccc gaccagcggc agccggaccc
     1681 gcacgcggtg ctcgccgtcg ccgtcctcgt tggacaccac cttgccgatc gcgaggccgc
     1741 tcgccgccgg taccagcgcg ccggcccgcg gcgcggatcc gtcgcgctcc tgggcggccc
     1801 acgcctcggt gcctccgaac tggacgtggg tcttccagcc ctggacgaga tcgaactcgt
     1861 ggcgcacgcc ggtgaccagc acgtcgccat tgtagcggtc gccgacgccg gccagcgtca
     1921 ccaggtcgcc ggggttgacc gtgccgatgc cttcgcactt ggccacaccg ctgaccttgc
     1981 tcatcttcga cttgagccac gcggcgtcgg cccacgcctg cgcctcgtcc tcggtcagtg
     2041 acgcgtgccg caggtcgtag ctgtcgagcc ccgcgaccgc cgacaggtcg tcgctggtca
     2101 ggttgcccgg gccgctgacc ccggggtcgg ccgccgactt ctcgaccacg gcctgctgcg
     2161 ccgggtccca ggtcctggtc ttgaccgcgc tgaactgcag gcgcgcgtcg atctgggcgt
     2221 cgagctcgag cagcgtggcg ccgaactgca gcgtgcacac cgccgagccg ctgaagctcg
     2281 gcgccttgac cgcgaccttg gcgtcgttgg tgaagaccag cttgccgttg gcctcggcgc
     2341 gcgccaggag aaaatcccag tccgacgccg cgagctggac ctgctgcttg tggctgacct
     2401 gcgtggcctc gacgtcggcg tcgacgcccg cgccgctcag cagcgacgac aggatgtcgc
     2461 tgtcggtctt gtcgaggtag tacgcggcct tgcgcccgac ggtgagcctc tgggccttgt
     2521 ggcggcactc gaccacgagc tgggtcgggc gctgatcgcg caccttgatc gactgccgca
     2581 cgatcacgcc gcggaacagc gaggtcttgt cgtcgcccgc gccggcgagg atctcgatct
     2641 ccttgcccgg gacgaagcgg tcggcgttgc tcagcggaaa gtcgccggtc gccgccgagc
     2701 catcctgata ggcgaggcgc gccgacgaga tccggttgac cgacttgatc acgctcatcg
     2761 ccagcagcgg atgctcgcgc ggcacctcct cgccgtcgac cttcaccgtg acctcgcggt
     2821 gcgacgccgg gatcgggagc gtgcgttcgt cggacatcgt cagttctcca gcggtggaaa
     2881 ccgcagccgc atgccgggga cgagggtgcg gaagtcgtcg agctggttgg cctgggcgac
     2941 ccgcaggtag aggtgcggat cgccgtagat ctcggtgcac agcgacggca ggctgtcgcc
     3001 ggccttgacc acgcggaagt gggtgaggtc gggcgactcg tcctgcgcca tcgcgacgcg
     3061 ggtctggtcg tcggagttgt cgctgaagct cgccgtgatc accgcgcgca gcgggacgcc
     3121 atcggacttg aacagcttgt aggcgatgct cgcgctcttg agcacgcagc gcttgatcgt
     3181 cagcttgccc cagccgatct tgaggtagtt cggccggtgg atgttgccgt tgtagccggt
     3241 cacggtctgg aactgctcga tcttgtcctg cacgtcgcac ggccggccgt tcgcgccggt
     3301 gccgtcgaga aacagcgtga gcgacagatc gcccggcttg accttcttga agctcatccg
     3361 gctgttcgtc gtgccggcgc cctgcgcctg gtcgtactcg atctcgtagg ccagcgtgat
     3421 ctcggtgggg ttgacgtagg cctcgaactc gtcgatcgcg tctcccgaat aatctgcctg
     3481 ctcgtaggcc cggatcacca ggcgctcgag ctcgccgctg tcgttgccgg tcccggccac
     3541 cgctcagcgc tcctgcttct gcgccaggat ctcgaggacg cgctcgacgc actcggcgac
     3601 cagcgcctgc ttgtcttcgg atcccgagga tcccgatccc ggccgcgcgc cccccgccgc
     3661 ggggttgtcg acgtccacgg agatcacgac ttcatcgacg aagatcggca tggctcacga
     3721 ccggtccatg gtgaagtatt gatagtagag ctggagggtc tcgatcacca ccgcgttgtt
     3781 cgcagcgttg agatcgctga ccgcccactt ggtggggtag gcgccgacga cgtgccaggt
     3841 cagcaggggc tggtgctcgt cattgagcag cttgacgtcg aggttctggg gctcgatctg
     3901 gtagtcctcg atgcaacgcc ggacccagcc gatgatctcc gagctcacga gcaggccgcg
     3961 cttgagcgtg agctcggggt acttcgtgcg gaccggatac ttctgcacga agcgctgctc
     4021 tccgccctcg gcgagctcct cggtgaccat ctcgtacgac agcccgccga cctcggtgaa
     4081 ccggacgtcg ttctcgttcg gcggcaggcc gacgaactcg accttgaagt gaaagccgac
     4141 gggcgggtag taggacggca tcactcgttc tgcagctcga ggccttcgtg ggcgagctcg
     4201 atcgactcga tcgcgacctc gttgccggac gccttgagct ggggtccctc gaccttgacc
     4261 gggaacgcgc gcatcacctt ccacaccatc accggctggt gctcctcgtt gagcaggttg
     4321 acgatcaggt cgcggcgctc gacggtgttg agcttgaccg tgctcagcca cttgaagaac
     4381 tggttgtcgc ccttgacgat gccgcgcttg agggtcacgt tcgagaactt gcgcaggccc
     4441 ggcatcttga tcgacgagta ctcggggaac gagccgtcgc ggtactcgat cgcctggttc
     4501 tcctgggtga ggccggagac ctcggagaac ccgacgcggt cgccgcccca ctggacggtg
     4561 aagtggaata cgggaatcgg atattgcgta ggcatggtcg acgctcctca gcggggaagt
     4621 cgagtgatca cgtgcgggtc gcggacggcg ctcacgacgt ctgcagcttg tgagagaact
     4681 tgaggacgat gaactcggcc gggcggacca ccgccatgcc gatctcgacg ttcatcctgc
     4741 cctcgaggat gtcctgcgcg ctcatcgtcg tgccgagccc gcacttgacg aagaacgcgt
     4801 ccttgggggt cgcgccggcg agcgcgccct cgcgccactt gtcgatcagg tagttctcga
     4861 tcatgccgcg caccttgacc caggtgttgg cgtcgttgga ctcgaacacc gcccagtagg
     4921 tcgacttctt gaccgactcc tcgaccatgt tgaagaaccg gcgcaccgag atgtaacgcc
     4981 actcgttgtc ctggccgagc agcgtccgcg cgccccacac caccgtgccc ttgccggcga
     5041 acgcgcggat cgcgttgatc gacttgccgg tgttggggtc gacgttcagc gtctcctgct
     5101 tgaggcggtt gagcttgacc accggagcca gcacgccgag cagggcgacg ttcgccggcg
     5161 ccttccacac gccgcgcgcc gcgtcggtcg cggcgtagac cccggcgatc gcgccgctcg
     5221 gcggcgtgtc gacgaaccag tccttgagct tggtcttgac gaagttgtac accgccggct
     5281 tggtggtcct gatcttgccc aggtcgtccg cggcaggagc gccaccacca gcgcctccac
     5341 cgccgccgcc tccgccacca ccacctccgc ccgccggcgg gttggtggtc accttgacgt
     5401 tgccctcgtc atcccggacg tagtggttca tcgtcgtggt gatgaatgga tagtacgcgg
     5461 ccccgtactt gaggtcgccg gcccaggcgt cgcgattggc ctggatgacg ttgaccttgg
     5521 tcccggccac ggtctcgacg ctggcgtcgt ccttctcgct gcccttccag acgtcgagga
     5581 tcgcgaagcg gtcctgcagc gtcgagcact gctgcagcat cgccccgcac aggtccttgt
     5641 agtccgcgta cccgagcctg gcggcctcgg gcacgatcag cagcgtcggc tcgtcctcga
     5701 ggccgatcgc ggcgagcccg tcgatcagct tggccttgtc gatcgcgccg gtgtagtcgc
     5761 cgaccgacac gatgtagcac tgatcgccgc cgttatcgaa gtacagcttc acggcgtagt
     5821 acatgaagta gctgacgccg ggtggcgtca cgttgcccgc gatcggcttg ccgtcgccgt
     5881 cgacatcgac gctcaccttg atgtcctcgg ccttcgggaa tccgaagtag gcttcgaacg
     5941 ccttcagcga gtagatcttg gtcggctcac ccttgaggtc gccggcgacg gtctgcgtcg
     6001 cctgctgggt gtagccgatg aacgccggga tcgcggtgcc aacctgggcg accgaaggtg
     6061 ggaacgtcga gatctcttct atgtagacgt cgggagcgcg gtaggttgcc atggtttgga
     6121 cctctagggc ttcgatttcg acagctggac gatcagatcg gcggtcccgc ggcgcttgcc
     6181 cggctggggc agccgctcga tgacagcctc ggtgttgcgc atgagctgga tctgcttcgc
     6241 tccggtctcc ctgcgcggga tgccggtcac cgaacggaac agcagcacct tggcgccgtc
     6301 ggtggcgagc acgtcggacc gggacttctc ctcggcggtg agctgatccg gcgccacctt
     6361 ctggaacgtc acgtcacccg gccccggtgc gcccgacgtc tgcggctgga ccgccagctg
     6421 atcgacgtcg ccgttggaga accccttgac caccacgtag taccgccagg tgtcgagccg
     6481 ggccttgaac cggatctgga agctggcggc gctggtgtag aaccccggat cgagggtgat
     6541 gtcgaccaga ccgagcgcgc cgtctcgcca cagcgcggga gcgacgtcgc ggagcacgac
     6601 gtgggcgggt ggatcgtcga gcgcagtcgc cacctggtga ttccggtagt ggagcgcccc
     6661 ggacgacgga tcgtggccat cggtgatgtt cgcgaagtac ggatcacgga tcaccaggcc
     6721 gaaccgcagg gtcttgccgg ccacggactg gaccggagcg ccggcgtcgt cgctgcggaa
     6781 caccacgtag agggttccgc ccacgacctt ggcgaacatc ccggcgccgc gcagggtcga
     6841 cgcggcctcg tcggggatga cgaagcccac gtccgggcag acaccgccgt agtaatcgtg
     6901 ccagacctcc agagaaaaca gctgtttgaa ccgggtcatc atgtcgcggt ggccgccgtc
     6961 tggatctcgg acaccggagg cgcgaccgcg gtgggctcga gatcctgcag ggccaccatg
     7021 cggaccttgt agatcacgga cggcagctgc ttggcaccga cgaatgtcca gatctggttg
     7081 agctgctcgt agctcagcgg ctggagctcg accgtcagcc gctcgatccg gggatcgagt
     7141 ccggagtagg tgtccggagt gaacgtcggg tgagactgga agtaggtcag gaccagagac
     7201 agtgatcgca gcccctcgtc gtacttctga aacttcgctg caaacaggat gtgaaggttg
     7261 agcttgagcg cgggctcgag aatcacctgc cggccgcggg caaggattgc ctcgggaagc
     7321 tgggatttca tcacccggtc ttcctcgatg tggatcagag aaaccccgat atggtcatcg
     7381 ctgatcaccc acttcccggc gtcgtccacc agcttaccaa cttcggcctt gccgatctcg
     7441 tccgtggact tccgggcgcc tcgggcgacg aggtacgcat tcagctctgt cgtcaagaag
     7501 ttgagcgcaa cgtcgagcat gcctatctgc cccttcacga aggcttgaca ttgcgacacc
     7561 cccccgggga atgcaaccaa cacaaccaac acaacccgag ctatgtcggc gacccgtgcc
     7621 gatccgatcg agtgcacgct atccgtgaac cggagggaga tcaagcgatt tcgggctgga
     7681 gcttcctggc gttggcttga taccgtgtcc ggactccaat cccgccatga aggaaatcaa
     7741 tttcaccgtg accatcgacg aggccaacct gatcctcaaa ggtatcgggc tcttgccgtt
     7801 cgcggacgtc taccagctgg tcgccaagct ccagcagcag gccgggcggc agatcgagcg
     7861 cgacaccggc aacggagagg atcgccgtcc ggtttccgtc cagggaactt gacgcccgcg
     7921 gggaggcgac gcgaagaaga gagcaacgtc gggcgcacgg gacctgactc gatcgcagga
     7981 agcaggtggt ggccttggcc cgagactttc cggatccccc gagcagcgaa cgcgacggca
     8041 cgagccaggc cggtcgcgct cagcccgcgc tcgacccggc gtacgtggcg gtcgacgagc
     8101 gctcggcccg cgacctcctg gtgctcgcgc acgcgtacac ccggcagctc aacttctacg
     8161 acgcgcagga tcggctcgcc ggagactgga ccggctttct cggcaacgac gacctcgagg
     8221 ccctcgcggc gcttgtagac gactcggggc cgccggggcc gagcgcggtc cggatggatc
     8281 gcccgcacgt cgcgctgttc ctcgctttcc tgcgcttgct ccgccacccg cgcgacctcg
     8341 tcaacaccct cactgcccgg catctcacgt tctactacac gcagctactg cagatgtcgc
     8401 gcacgtcggc agtcggcgat cgcgtgcacg tgctggtcaa gctcgccacc ggcgtcgatc
     8461 aggcgcgact gccgcaggga accgcgctgc gcgccggcaa ggacagcctg ggccgggagc
     8521 agatctaccg gaccgatcgc gagctcgtgg tcagccgcgc gaagctcgcg cggctgtcga
     8581 gcgtctacgc ccagaaggag atcgtggggc tccgcgaggc ccgggaacat gccaggggca
     8641 ctcgcgagga ggcattcctc gcgatgctcc ggatcgcgct cggcgctccg cgtcccgggg
     8701 atccgttgcc gcagtacggc ggcaaggacg tgacctatcc gctcctcctc gagctcaagc
     8761 agcgcgtgga cttcgctgcc gcgagcctgt tcatggactt ctcggatctc cacgcgatga
     8821 tgcagctggt gcggcgtcgc gccgccgatg ccggcgactg ggccacgatc aatgccatcc
     8881 tggagaaggc cggccagacc cgcctccacg atccgacctg gaagctgaat cccgccaacc
     8941 cgcgggattt ctccgcgaac ctggccaagg ccctcggcgg cgcgccggac ttccagcgcg
     9001 acggcctgtc cggcgtcaac agcctcgccg atctctacta tcagctgttc cgggcggacg
     9061 tgatcgccta cgtccatgac aagctgttcc tgtccgaggc cgacttccgc gcgatgatgg
     9121 gagtcaagat cccgatcgac gcgcagtggg gcgagatcga tcgcatcctc gagcaggccg
     9181 gccggcggcg ctggaacaac ccgacctacc ggttacatcc cgctgatccc acggcgttcg
     9241 cggcgaacct gcaggccgcc atcggaccgg ttccgttcag cagtcttccc cacgcgatcc
     9301 ttcccgcgat cgccgacatc ggcgcctatt acgacgcgat cctcgcgctc gaagcgtact
     9361 tccacatgaa cgccgcgcag ctcggatacg tgctcgccac ggccgagaag ccggcgccga
     9421 gcgcgacgcc gcgcgagtgg gacacggtct atcgcatcct gtccgacgcg caccgcgacc
     9481 tggtcaacgc gcagcgcgac cagctcgatg ccgaccgccg gcaagcgctc cagaaggcgc
     9541 gcgaggccgc gcccaacgcg gtccaggggt ttctcgcggt gctgcggatc gcgctcggca
     9601 tgggtgcggc gtcccagccg tcgctggccg atctgcgacc cgacgtccag aagcccgagg
     9661 actactcgtt cctcgaatcg gtccagtccc ggctggtgag cgggacgatc acgcccgccg
     9721 agtggtcgcg ggtctaccag gtggtcgtgc tcgcgcagcg cgtgcgcgac cagctccccg
     9781 acccggtcgc gcagcgcgag acctggctcg acctgcgtgc cgccgacgac gcgacggcga
     9841 tcgccgccgg acccgcggag ctgccgcggt ggaggacgtt cggccagacg gcgccggtca
     9901 cgagcaccga cgcgccacat ccggccctga cggccctgat cggctgggcg atcagctccc
     9961 cgctgctggt ggtgacccag gggacgcgca cgctgaccct gacgctcggc gtgccggcca
    10021 aggacttcgg caaccgcggg atcgtccggc tgctcccgcc gtacgcgccg ctcgaccagc
    10081 tgccgttcga gctccagatc agcaccgaga agggctggct gccgctgacc gtcagcgcgg
    10141 tcgtcggcga ctaccggacg ctgagccagg tgtcgcgcgc ggtgcccgac gcgccgcagg
    10201 cgatccagct cacggcgacc ctcgccgacg atgcggcccc ggccgcgccg aaccagacca
    10261 ccgcgtgggc gggcgggtac cccgtgctcc ggctcctgct gcgccggatc tgggatgcca
    10321 gtcgcggaca gtacatctcg ctctacgagc cattccgcga cctgccgctg gtcgccgccc
    10381 acctgcgggt cagcgtcacc ggcctgacac cgtcgtcgat ccagaacgac gatgcggtgc
    10441 tcaatgccag gaagccgttc gagccgttcg gcagccggcc cgcggtcggc tcgcggttct
    10501 acgtcgccga ccccgagctc gtgatcaagc gcctcgatgg cgtggccttc catatcgact
    10561 ggatgggggt gccggccgac ctggcgcagc actacgcgaa ctacgacgtc gccgggcagg
    10621 cgttcagcgg cacgttcacg gcgcaggtca gcctgatcga caaccacctg cgcatgccgc
    10681 tcagcaccac cccgagcagc ctgttcgcca gcggcgaccc gaccaagcgg caggacctcg
    10741 cgttcgcggg gctgcccacg atgctcgagc gcgccggcta ccactacgcg ccgtcgtcgc
    10801 ttccgcccgg cggtcccgag gtcacggcgt ggccgcgcta cttcgagtgg gagctgacgc
    10861 cgaccgactt caagcaccag gcctaccccg cggcggccgc gcagcgcgcg ctcgagctga
    10921 ccgcggcgct gatcaagacg ccagccaccg ccaacccggc gagctaccag gtcaagccgc
    10981 catacacgcc gaagatcaag gcgctgcgga tcgactacgc ggcccagacc gaggtggtcc
    11041 tcgacgccgc gcaacccgcg gccgggaccg accggctgtt ccacatccac ccgttcggcg
    11101 ccagcccgat cgccgccgag gccgacgcga ccggcacgcc gctgctgccg cgctacgagc
    11161 acgagggcga gctctacctc ggcatccaga acgcccggcc gccggagacg ctgtcgctgc
    11221 tgttcgagat ggccgatggc agcgccaatc ccgatctgcc gccggtgaac atcgagtgga
    11281 gttatctcag cggcgacgcc tgggtcacgc tgcaggacgg ccacgtcctg ctcgacacga
    11341 ccaacggcct gatcgactcg ggcatcgtgc agatcgcgct gccgccggcc gacccgagca
    11401 cccggctacc cggtgatctc tactggatcc gggccgcgat tgccaatcac ccggacagcg
    11461 tatgcgacac ggtgggaatc cacacccagg cggtgtcggc gacgttcgac gaccacggca
    11521 acgcgcccga ccatcacgcg cacgcgctcc cggcgcgcag catcgccggc ctggcccagc
    11581 gcgtcccgca gatcgccggc gtcgagcagc cctacccgtc gcgcggcggc cgggcggccg
    11641 aggccgagcg cggcttcttc acccggatca gcgagcggct ccggcacaag cagcgcgcgc
    11701 tcacgccgtg ggactacgag cgcctggtgc tcgagcggtt ccccgggatc gggaaggtca
    11761 agtgtctccc ggccgatccc gacaacctcg gcgcggtcca gatcatcgtc attccgcaga
    11821 tccagggcca gtcgctggcc aaccggttcg agcccaaggt ctccgccgcc cagatcgccg
    11881 acatcaccgc gtacctcgcc ggccacagcc cgccccacgc ggcgctgcac gtgcgcaacg
    11941 cgcggttcgt ccaggtcaag gtccgggtcg gcgtccggtt tcagggcacg ggcgacgacg
    12001 gcttctacag gagcctgctc gacgacgagc tcgttcgcta cctgtcgccg tgggcgttcg
    12061 acgacggcgc cgacatcgcg atcggcggca agatcttcgc caacagcatc gtgagcttcg
    12121 tcgacggccg cgactacgtc gactacgtcg cgaacatcga gctgttcagc agcaacgacg
    12181 gcggacgcac gttccagctc gcggtgccgc agccgtccca gggctacttc gtgtccaccg
    12241 atcagcccga cgcggtcctg gtcacggccc cggaccacga catcgacatc attccggaca
    12301 cgggatacac ggccgtgtcg ttcaccggca tcaactacat gaagatcgag ctcgacttca
    12361 tcgtcgcggg ctcggcgtga cttgcgaact tccagcgcac atggagcacc tctatgccgc
    12421 agctcaccac gcgaccccgg ggcgacctca agacgttctt cgccaagaac gcgatcccga
    12481 ccgagagcaa cttcgccgac ctgatcgacg ggatgctgaa ccagcaggac gacggcatcg
    12541 ccaagctgcc caacgacgcc ctgatgatcg tcgccgcggg tgacgcgacc agccagaaga
    12601 aggcgctcca cttctattac gccgcggccg acaccgatcc gagctggacg ctcagcctca
    12661 acccgcgcca gaatcccgcc gccgcgaaca ccgccaacct cggcttcagc atcagcgacg
    12721 gggccgggaa tagccggctg ttcatcgagc gcagcaccgg ccggatcggc gtcggcacgg
    12781 tcggcccgct ccaggcggcg ctgcacatcg accgcggcgc gaccaacgac ctcgccctgc
    12841 tgctgagctc gtccggcccc ggctggggct cggggctgca gctcaagaac accgcggccg
    12901 gcgccgcgca tacctacggc atctataccg gaggcggaac gctgcacttc gccgacgtcg
    12961 acgcctcggc cgatcgcatg gcgatcctcg gagacggcac ggtgtgggtc ccgggcaagg
    13021 tcacgatcgg cgtgagcaca ccggtgagcg acctgacgct gcgccgggat gccacagctg
    13081 ccctgggccc gatcatcacg ctgtacaaca tggctggagc tgcgggagcc ggcggcgcga
    13141 tcgatttcaa tggctacaat gtcggcgcca acccgcccac ggcgcgcatc cagtcgctcg
    13201 acgatggcat cttctcgagc cacgtcgtga tctcgaccaa gaactcgggc gccgtggcca
    13261 atccgctcgt cgaacggatg cggatctcga gcggcggcat cgtgtcgttc accggcgcga
    13321 tcatgccgtc ggccggcaac tcctcggtgc gcgggatcca gttcccctcc aacccgggcg
    13381 gcggcggcgg ggatgaagcc ttcatccgct actacccggt ctccggtgag acctgcaagc
    13441 tcctgatcgg catcaacaac gacgccgacg acacgatcgg cctctaccag gccggggccg
    13501 agcggctgac cgtgggctac ggctatgtcg acgtcgtcgc cgacggcaat ccgatccggt
    13561 tcacctcgtt ctggactggt ttcgccgaca gcgcgaccaa ccgcgccgag atctcgaacg
    13621 acaccggcgg ctacaagacg ctcatgatcg tcggcaacaa gtcggccggc cacgagcgcc
    13681 gggtcggggt ctgggaccgg ctcgacgtca acggctcgtt ctgggtcaac ggcgcgcaga
    13741 agttcgacct cgccgaggtc acgccggcgc gcgccgacga tcagctcgag cagggcgacg
    13801 tcgtggtgat cgatcgcgag gacggcctgc gggtgcggcg cagcgcgaga ccccatgacc
    13861 cggcggtgta cggcgtcgtg tcgagctacg cccaggctgc gatggtgatc gggggctccg
    13921 gcgacccgga cgagatgacg caggcgcccg acaggcggcc gatcgcgctg gtcggccggg
    13981 tcaagatcaa ggtctgcagc gagaccggcc cgatccgggc cggcgatctg ctgaccacgt
    14041 cgtcgcggcc gggccatgcg atgcgctgcg ccgatccggc ggcgcacccg ggcgcgatcg
    14101 ccggcaaggc gctcgagccg ttctccgggg acaccggcac catcacggcg ctcgtgacgc
    14161 tgcagtagcg ccatgagcga cccgacgtcc gacaccgccg agctgtccac cgcgccgttc
    14221 gagcacccgg cgctcgacta cagcctgctg cgcagcgagg gcatccagca cctcgagcgc
    14281 ctgatcgggc acgtgtggaa tgacttcaac ccccacgacc cggggatcac gatcctcgag
    14341 cagctgtgct acacgatcac cgatctcgcc taccgcgcgt cgtacgacgt ccccgacctg
    14401 ctcgcgagca gcggcggcga tccgtaccgc agcctgtacc gcccgtccga gatcctcacg
    14461 tcgcatccgg tgacgctgct cgatctgcgc aagctgatca tcgacgtcga cggcgtgcac
    14521 aacgcgtgga tcgaaccgat cgccgagccg gcgcccgcgc tgtactaccg cgatgacaag
    14581 cgcgagatcg gctttcgggc cgatccgccg tacaccgttc cggtcggccg gcaagggcgg
    14641 tttcgcgcgc tgatcgagac ctcggacctc gtcgatcgcg gcagcgacgt ccagcgcgac
    14701 gtggcccgcc ggctgtcgac ctgccggccg ctgtgcaccg acttcgagct ccaggtgctg
    14761 gtcaaccagc cgatccaggt attcgccgag gtcgagatcg ggccggtcga cgacgcggcg
    14821 agcgtgctgc tcgcgatcta cgagcggctg accgagcaga tctcgccatc ggcgccgttc
    14881 cagacgctcg gcgagatgct ggcggcgggc aagcgcagcg acgagatctt cgacgggccg
    14941 ctgctcgacc acggcttcct cgacgccggc gcgctcgccg gcatgacccg caaggacgcg
    15001 atccacgcct ccgatctgat ccgcgagatc atgcgcctcc ccgaggtcaa ggcggtgcgc
    15061 agcatcgcgc tggccaaggg caccgccgcg cgcgagccgt ggagcctgtc gctcgacccg
    15121 acccagaccc cgagcttcga tcccagaggc tcgtcgatcg tgctggtcaa gggccagctc
    15181 accgcgaaga tcgacaccca gcgggtgatc gacacgctgc tcgagcgccg ccgccaggcc
    15241 gcgtcgcgca gccggctgtc tcccgaggac cgcgacttcg ccctgcccgc cggccgcgac
    15301 cgcaacatcg ccctgtaccg ttcgatccag caccagttcc cggcgctgta cggcatcggc
    15361 accgccggac ttccggcctc ggtctccgac cggcgaaggg cgcaggccag gcagctcaag
    15421 gcctacctcc tggtgttcga tcagctgctc gcgaccagcc tcgcgcagct cgcccatgcc
    15481 ggatcgctgt tctcgttcca cgccgacgac ccgcgcacct acttcacgca gccggtcgag
    15541 gacgacgcgc tcgacctgtc cgagatccgg acgcaagaca ccggcgacga cctcgcgcag
    15601 ctcgacgacc cggcggggtc gccgtcgagc cgccgcaacc ggttcctcaa ccatctgagc
    15661 gcgcggttcg ccgagcagtt caccgactac tcgctggtcg ccggccaggc cccggggtcc
    15721 gacggcgatc gaggggtccg cgacaagcag gcgttcctcc agcactaccc ccggatcagc
    15781 agcgcccggg ggaccgggat ggacgccgcg tcggcggcga gcgagaccga tctctcgggc
    15841 ctgcaggagc gcatcgagcg caagctcggc ctgtccgcgg ccgacggcga gcggctgctg
    15901 gtcgtcgagc acctcctgct cgcgccgatc gcggaggaca acgtcccgcc cggcaagctc
    15961 gattacgccc agactcccat cctgtcgggc gcggtcgcgc gcgatccgta ctcgctgcag
    16021 ctcagcgtga tcgcgagctg gcgcggacgc ctgatcgggg cgcgcgacac cgccgacgag
    16081 ctccgcgtgc tcgtcgagca caccgtgcgc gaggagaccc cggcgcacct gacgccctac
    16141 ctgcactggc cggacgagac ccggtggccg gcgttcgacc gcgcctacca ggagtggcgg
    16201 gatgcctacc gcggctacag cgcggcgaag ctcgacctgg cgctcgccga tgacccgagg
    16261 tacttccgca tgcgagacgc gcgcgatcgg atgatcgacc tgctcgggtt cggcaagacc
    16321 tatccgctgc gcgacctgcc ggtccgcgac gagcggctca ccgtcccgtt caaccagccg
    16381 gcggggatcc cgatcgatgc cagccagcgc ggcgtgacct acgtgctgcg caacgatcag
    16441 ggcgacaagg acccggggcc cgcgccgatc accgccgacg gcaccggtgg gacgatctac
    16501 ctcgagaccc cgccgatgcg cgacgacacc acgttcacga tcctggcccg caagctgtcg
    16561 ccgacccgcg aggtgttcct gctgcatcag cccgtcgtga aggtcgggct cgacgtcacg
    16621 ctccgcgccc ggacccgggc cgatccgctc gatccgagca tcgagcagcc cgccgacgac
    16681 gacgcccgca tcgtcgcgtg gggcacccgg gcgaccgtgc agatcgatca cagccaggag
    16741 ggcgtcgact accacctggt ccagatcacc ggcacgcagg agaagccggt gtcgcccgac
    16801 gtccgcggca acctcggcaa catcgcgctg accgccgacg ccgcgaccga ggacgtcgat
    16861 ctccggatcc gcgccaccaa ggtgttcgat ccgtcggagc atcgcgacac ccagaccgcg
    16921 ctgctcgcca tcgtgctgcc gctccgggtc cgcgcccggc tcgacctgcc ggtcgcgccc
    16981 gagccgtcgg cgctgatcga cttccgcgcg acgccgagcg tccggatcga cagcacccag
    17041 gcgagcgcca gctatgcctt gtacgtccgc gcggtgtcgg atcgcgacta cgtgttcagc
    17101 gcgccggtcc cggggctgct cgccgccgac gtcgacggcg cgccgcgggt ctacgtcacc
    17161 cgtccgccgc agcgcccgat ctggcaggac ggcgacggct tccagcccgc cggagcggcc
    17221 gtccagggca ccggcggcac gctgcacctg ccgctccccg cgctgcgcga cgacagcgtg
    17281 atcgccgtcc gcgcccacaa ggaccatcgc cagggcgcgg cgacccttcc gtcttcggtc
    17341 cagctcgccc aggccgcgct gatcctggtg cggcccgatc cccagccggc gatccagctg
    17401 gcagtgggta tcgacggcgg caagaccacc ggcacggtgg acgtcaccgg cagtcagccc
    17461 ggcgtgtact acgagctccg gcgcgacacc gacggtccgc tgctcggcct gccgggctac
    17521 ttccacaaga ccgacgagca ggcgccggcc gccaacaagg gcatcggcgc cgaccccgcc
    17581 tacggcctgc ggatcggggt cgacctcgcg atctcgcgcg gcacccagcg gccggcggcc
    17641 acgcgcgacg agctcgccg
//
LOCUS       DASVOR010001950         2434 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_137438,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001950 DASVOR010000000
VERSION     DASVOR010001950.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137438"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VF516_36220"
     CDS             <1..1390
                     /locus_tag="VF516_36220"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEX8113251.1"
                     /translation="QLSWKSLLDTYACTECARCTNYCPAYNTGKNLSPMQLIHDIRYE
                     MLDRTALRDRIAELEAEIAGLGEYASSHGFDDAHPHPDLAFARERLAAAQAELEAMPK
                     LTGGRVQEDTLWACTTCGACQEVCPVFIEHPVKIIQMRQNLVLEQEKTPGELQRTFRN
                     IERQSNPWGIGADQRMDWANGLDVPTIEDHPDPEYILWVGCAGAFDKRIVKQTRAMVK
                     ILDAAGVDYAVLGHQEACTGDPARRAGNEMLFQALAEQNVETLKSVNAKKIVTSCPHC
                     LHTLRHDYPQFGGEFEVVHHTQLIDHLYKTGKLASEKAPVRTLTYHDSCYLGRWNREF
                     DAPRNIVRTVLASASASGGGDGATGSSDGYREMTRSKRHGFCCGAGGARMFMEETEGE
                     RVNVNRTDEVIAANVEAVAVACPFCNIMLTDGMKQRNVEEKIQVLDVAELVAASIPDV
                     PVDRLVRKKSEG"
     gene            1720..2061
                     /locus_tag="VF516_36225"
     CDS             1720..2061
                     /locus_tag="VF516_36225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113252.1"
                     /translation="MCLGYAGCGTDDKQGTRAECAQGGALTDCPDAERTSQGACWRLV
                     DCGAIAVQSSNTNRFSWGRCVDSLDGLTADRQRLVIDCVAASTCDELRVDGSPDNPDP
                     HDMHCLRFGGL"
     gene            2061..>2434
                     /locus_tag="VF516_36230"
     CDS             2061..>2434
                     /locus_tag="VF516_36230"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8113253.1"
                     /translation="MSSRSQPLSAAAAAALAVVVALVAAAAPAVAQPADVAAQIKLIE
                     TQPANMDRATWKDRRRDAARRLGQSKDRRAVAVLIRLAETETFDIIGEIAIEGLGNLG
                     DPAAVPVLQRILGDPSRDKAQK"
BASE COUNT          422 a          850 c          845 g          317 t
ORIGIN      
        1 gcagctgtcg tggaagagcc tgctcgacac ctacgcctgc accgagtgcg cgcgctgcac
       61 caactactgc ccggcgtaca acaccggcaa gaacctgtcg ccgatgcagc tgatccacga
      121 catccgctac gagatgctcg accggaccgc gctgcgcgac cggatcgcgg agctcgaagc
      181 cgagatcgcc gggctcggcg agtacgcgag ctcgcacggc ttcgacgacg cgcatcccca
      241 cccggatctc gcgttcgcgc gcgagcggct cgccgccgcc caggccgagc tcgaggcgat
      301 gccgaagctg accggcgggc gggtccagga ggacacgctg tgggcgtgca cgacctgcgg
      361 cgcgtgccag gaggtctgcc cggtgttcat cgagcacccg gtgaagatca tccagatgcg
      421 ccagaacctc gtgctcgagc aggagaagac gcccggcgag ctgcagcgca cgttccgcaa
      481 catcgagcgc cagagcaacc cgtggggcat cggcgccgat cagcgcatgg actgggccaa
      541 cgggctcgac gtcccgacga tcgaggacca ccccgatccc gagtacatcc tgtgggtcgg
      601 ctgcgccggc gcgttcgaca aacggatcgt caagcagacc cgcgcgatgg tgaagatcct
      661 cgacgcggcc ggcgtcgact acgccgtgct cggccaccag gaggcgtgca cgggcgaccc
      721 ggcgcggcgc gcgggcaacg agatgctgtt ccaggcgctc gccgagcaga acgtcgagac
      781 cctgaagtcg gtcaacgcca agaagatcgt cacgtcgtgc ccgcactgct tgcacacgct
      841 gcgccacgac tacccgcagt tcggcggtga gttcgaggtc gtccaccaca cccagctgat
      901 cgaccacctg tacaagaccg gcaagctggc ctcggagaag gccccggtgc gcacgctgac
      961 ctaccacgac agctgctacc tcggccgctg gaaccgcgag ttcgatgccc cgcgcaacat
     1021 cgtccggacc gtgctcgcct cggcctcggc atctggcggc ggcgacggcg ccaccggcag
     1081 cagcgacggc taccgcgaga tgacgcgcag caagcgccac ggcttctgct gcggcgccgg
     1141 cggcgcccgg atgttcatgg aggagaccga gggcgagcgg gtcaacgtca accgcaccga
     1201 cgaggtcatc gcggccaacg tcgaggcggt cgcggtcgcg tgcccgttct gcaacatcat
     1261 gctcaccgac ggcatgaagc agcgcaacgt cgaggagaag atccaggtgc tcgacgtcgc
     1321 cgagctggtc gcggcgagca tccccgacgt cccggtcgac cgcctggtcc gcaagaagtc
     1381 ggaggggtga agagctgtag aggcaaagga gcggacctgg tcccgcagac gtcgagcttg
     1441 acctcgacgt cgacgcaagg cgttgagctt gacggcaacg gcgacgtcta ccccccaccg
     1501 ttgacgtgga acatggaccc agcactgccg acgagaccat cgtcgagaag gtatcgggag
     1561 ctcaacgtcg aggtcgacgg tggtgtcgac gtccaacgtt gccgtcgacc tccacgttcg
     1621 ggtcaaggtc atcgtcaagg tcatggcggg acggcagcgc ggggccgcgg cgcggagtta
     1681 taaccgcgag cgatgacagg acgtcttgtg atcgcggtca tgtgcctcgg gtacgcgggg
     1741 tgcggcaccg acgacaagca ggggacgcgc gccgagtgcg cgcagggcgg cgcgctcacc
     1801 gactgccccg atgcggagcg cacctcgcag ggcgcgtgct ggcggctggt cgactgcggc
     1861 gcgatcgcgg tccagtcgag caacaccaac cgcttcagct ggggccgctg cgtcgacagc
     1921 ctcgacggcc tgaccgcgga tcgccagcgc ctggtgatcg actgcgtcgc ggcgtcgacc
     1981 tgtgacgagc tccgggtcga cggctcgccg gacaacccgg acccgcacga catgcactgc
     2041 ctccggttcg gaggcctgtg atgagctcgc gatcgcagcc gctctcggcc gcggccgcgg
     2101 ccgccctcgc ggtcgtcgtc gcgctcgtgg cggccgccgc gccggccgtt gcgcagccgg
     2161 ccgacgtcgc cgcgcagatc aagctgatcg agacccagcc cgccaacatg gaccgcgcga
     2221 cctggaagga ccggcggcgc gacgccgcgc gccggctcgg ccagagcaag gatcggcgcg
     2281 cggtcgcggt gctgatccgg ctcgccgaga ccgagacctt cgacatcatc ggcgagatcg
     2341 cgatcgaggg cctcggcaac cttggcgacc ccgccgcggt gccggtgctg cagcggatcc
     2401 tcggcgatcc gtcgcgcgac aaggcccaga agga
//
LOCUS       DASVOR010001951         3597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_282575,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001951 DASVOR010000000
VERSION     DASVOR010001951.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3597
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282575"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..479)
                     /locus_tag="VF516_36235"
     CDS             complement(<1..479)
                     /locus_tag="VF516_36235"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8113254.1"
                     /translation="MSFPRIAIVGAGLGGLVLARILRRHGVASTVYELDAGPDARRRG
                     GLLDLHVGSGQRALHDAGLLEQFRANTLPQGEHVRVMDKAGTVFIDRGPEGGEGLRPE
                     IDRTVLRNLLLVSLPPGAVAWGHKLTAAERAEGGGFHLAFTDGRTAWAKLLVGADGA"
     gene            1085..1321
                     /locus_tag="VF516_36240"
     CDS             1085..1321
                     /locus_tag="VF516_36240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113255.1"
                     /translation="MVRVNGHGNIHADGWRAYVNHALAQYTVGLEFREHEVRVWFFDV
                     LLGAFDPRTQQSVEPITPAPTRAQLDQDPRCSGQ"
     gene            complement(1657..1941)
                     /locus_tag="VF516_36245"
     CDS             complement(1657..1941)
                     /locus_tag="VF516_36245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113256.1"
                     /translation="MITGRDAQETSESPVENFTGHARVQRLSLPTPPAHMQGAYVTFE
                     PVVWTPPGVKHWHGATPTTAMTHMALVEQLDGKSATWMEKVTDTQYNRAP"
     gene            complement(2138..3136)
                     /locus_tag="VF516_36250"
                     /pseudo
     CDS             complement(2138..3136)
                     /locus_tag="VF516_36250"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            3027..3524
                     /locus_tag="VF516_36255"
     CDS             3027..3524
                     /locus_tag="VF516_36255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113257.1"
                     /translation="MTDDASSNASSTLLLRPRRAKWLLLLLASLALTVAGISMIRGGE
                     TKGWFVVAVFALSTLVSGTMLLPGSAYLRLTPDGFEMRSLFRHSRTRWVDVTWFRPGR
                     IGLNKMVMFDYAPSYTRSAKARALASALAGTEAALPDTYGHSAEALATLLNDWRARAT
                     RGAAS"
BASE COUNT          581 a         1216 c         1220 g          580 t
ORIGIN      
        1 caggcgccgt cggcgcccac gagcagcttg gcccacgcgg tgcggccgtc ggtgaaggcc
       61 aggtggaacc cgccaccctc cgcccgctcg gcggcggtga gcttgtggcc ccaggccacg
      121 gcaccgggcg gcaacgagac gagcaggagg ttgcgcagga cggtccggtc gatctccggg
      181 cgcaggccct cgccgccctc cgggccgcgg tcgatgaaga cggtgcccgc cttgtccatc
      241 acccgcacgt gctcgccctg cggcagggtg ttggcccgga actgctcgag gagcccagcg
      301 tcatgcaggg cccgctggcc ggacccgacg tgcaaatcca gcaggccgcc ccggcggcgc
      361 gcgtccggcc cggcgtccag ctcgtagacg gtggacgcga cgccgtgccg gcgcaggatg
      421 cgcgccagga ccaggccgcc gaggccggcg cccacaatgg cgatgcgggg gaaggacatg
      481 gggctcccgg cggtcgaagg aagtgtacag cgtaacgaca gctacgctcg ggatcccaac
      541 gcttcccttg ccgtcgacgg cgcgaaggcg tcaagcgccg cgaggaatgc atccaggtgt
      601 tggcgagccc gcagcgggag ggctgagtcg aggttatctt gacatctccg ttatccggag
      661 cagtccgcat gtctcgacga gacgacgacg gcaccgatcc agatcacacg ccgctcgtaa
      721 gtgcggtcat gcggccacag ctccaggcgc gcggcccttc gcggtcgaga ccagcgatct
      781 ggatcccatc cggtagctga cccagagcac gccacgacct tccccagacg acacgctggg
      841 tcaacaagac gcgaactaca ttaggcgagg gcacgcgaag tccggagtca gttctggagt
      901 ggagtgttgc gagcgcgcgt aacgaagtgc accgggaaca tgatggactg atgactggct
      961 gacgacgttc aaccaggtac gtccccacga agcgctccaa cagcgcacgc ctgccgaggt
     1021 atatcgggtc agcacgcggc ggccgacgcc acgcacgatc ggcggcttcc ccgagggctg
     1081 ccgcatggta cgcgtcaacg ggcacggcaa catccatgcg gatggctgga gagcctatgt
     1141 gaaccatgcg ctcgcacagt acacggtggg gctggagttc cgcgagcacg aggtccgcgt
     1201 ttggttcttc gacgtgctcc tcggagcgtt cgaccctcgc acgcagcaat cggtagagcc
     1261 catcactccg gcaccgacgc gggcgcagct cgatcaggat ccgcggtgca gtggccagtg
     1321 acgcacgggc cgctgccgcc attgccgcga gtcttttctg ttgagcgctc gattatgtgg
     1381 agctggaccg gaatgccgct gccgtgtcga cgaggccggc gcgggtgaac tccgcataat
     1441 gcgctgcgct ccaaccgcag caggagcagc agcggcggcg gccggcagac gcgcgccttg
     1501 gacctcgatg ctggaagccg cagcccggat gtcgcgcagc tcctcgggcg tcagctcgat
     1561 gtccacggca ccgaggttct cgtcgaggcg gtgcagcttg gtgcgcggac acttcgaggc
     1621 tcgtacccag ctttctgttt cgcatcttcg gctccttcag ggtgcgcggt tgtattgcgt
     1681 gtcggtgacc ttctccatcc acgtcgcgct cttgccgtcg agctgctcga cgagcgccat
     1741 gtgcgtcatc gcggtcgtcg gcgtggcgcc gtgccagtgc ttcacgccgg gcggtgtcca
     1801 cacgacgggc tcgaacgtga cgtacgcacc ctgcatgtgg gccggcggtg tcggcagaga
     1861 caggcgctgc acgcgcgcgt ggcccgtgaa gttttcgacg ggactttcgc tcgtttcctg
     1921 ggcatcgcgc ccggtgatca tgatgtcctg actcgcagcc tcgggcgccg tcggtgccag
     1981 gtgactcgac ggtcgggatg tacacgccag cgtcacgagc aacgcgagcg ttgtggctcc
     2041 tgtcatccgc atgccttcgt ctaccagccg cggcgaaggt gcagtacatg cggatcccgc
     2101 aaggagttat gcacgcggcc catcaatggg ctcgatgtca tcggcgcgca ggtgggcggt
     2161 accgcaaggc atccacgacc agcgaaaacg cgggcgacgc ctgcttacga ctggcgaaat
     2221 acagatggaa gcccgagaac ggctgcgacc agtcgtcgag cacgaccttg agctcgccct
     2281 ttttgatgta cggatcggct tcatcttcgg gcacgaagcc gatgccgatg cctgcgcgcg
     2341 ctgcgacgag catctcgtcg atgccgttga ccgtcacctg cccttccacg cgtaccttga
     2401 cctcgcggcc agacttctcg aactcccacg catacgccgc cggatagccg gaggttgata
     2461 caacaatggg tggtcaggtc ttgcggcttc ttcggcggcg gatgctccgc gaagtagctc
     2521 ggcgtaccga ccgcgaccag gcgaatcggc ggcccgatcg gaccgcgatc ataccgcggg
     2581 cgagatgctc gccgaggcgc acgccggcgt cgtggcgctc cgcgacgata tcggagagcc
     2641 cgtagtcgac gacgatctcg accttgatgt cgcgatatgc gcgtaacagc ggtgcgagct
     2701 tcgggagtaa gatcgacttc gccgcgtgat cgctcgtcgt gatgcggacc gtgccagccg
     2761 gctgtgaacc gcaggcgccg cccgccgagt tgctcgcgga ggactcggtt ctcctcgcgc
     2821 aggaagtcga tgacctcaag ctggcgggag tggatccagc ccgccatcca gaggaccaga
     2881 aaccgcacag gctcggtacc catcgcggcg agcccggctg gtgatccacc tgggcgtgcg
     2941 acgagttacg acaagtcgcg gagatgcctc gcgtttccgg ctggacgact ttcggcacac
     3001 ggcgccgtgt cacactcgtc gaggcgatga ccgacgacgc ttcgtccaac gcctcgtcca
     3061 ccctgctgct gcgcccccgg cgcgccaagt ggctactgct gctgctcgcc agcctcgcgc
     3121 tcaccgtcgc cggcatctcg atgatccgcg gcggcgagac caagggctgg ttcgtggtgg
     3181 cggtgttcgc cctgagcacg ctggtgtccg gcacgatgct cctgccgggc agcgcgtacc
     3241 tccggctcac cccggacggc ttcgagatgc gatcgctgtt ccgccactcg cggacccggt
     3301 gggtggacgt cacgtggttc cgccccggcc ggatcgggct caacaagatg gtgatgttcg
     3361 actacgcccc gtcgtacacc cggtccgcca aggcgcgcgc gctggcgtcc gcgctcgccg
     3421 gcaccgaggc cgcactgccc gacacctatg gccactccgc cgaggcgctg gcgacgctgc
     3481 tcaacgactg gcgtgcccgc gcgacccgcg gcgcggcgtc gtgacagcgg cggtcactgc
     3541 cagaggaggg tgaacggcag ccgggcctgc ggcttgctct gatcgaccag cagcgac
//
LOCUS       DASVOR010001952         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_577084,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001952 DASVOR010000000
VERSION     DASVOR010001952.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577084"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..482
                     /locus_tag="VF516_36260"
     CDS             81..482
                     /locus_tag="VF516_36260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113258.1"
                     /translation="MLALARRGLEVWAVDTGALAPQVAALPSVHHLAKKVGALRWEEL
                     PAQIDWLLVDVNLAPQVALHEVRRLMPRLVGTLRGAVFTLKLNDWAFVAELPALVDRI
                     RQMGLDDVRLRHLPSNRREVCAVAQHASARL"
     gene            743..1108
                     /locus_tag="VF516_36265"
     CDS             743..1108
                     /locus_tag="VF516_36265"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8113259.1"
                     /translation="MTDHDHTSHSYSVEISALVRVLGDRVRQQVCQLRSISLGGTFIE
                     VGRLPMGTQVNITFGLPTIGERISLDAVVHWSTDYGVGVQFEGMRARQVWALWRYLES
                     VTGAEPEAWLGDTKEIIMN"
     gene            complement(1143..>1827)
                     /locus_tag="VF516_36270"
     CDS             complement(1143..>1827)
                     /locus_tag="VF516_36270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113260.1"
                     /translation="DPAVLAVLATSLYRIGEFVACEPVAQQLIGHADRATRLAGLHAM
                     ARALAGQGRHVDALRYAKTAVELAQGDAIEPGDAAELAETLDRIVAQEVPPVRASAEL
                     SLERQACDELEAGKFDSLIAAVSSPSWGIAHAALAACEVRRDDEGGIPVSPRALDAAV
                     EILARSAGATQPDAVLARIRALRIRDNAFIQSDPPPPLGTRYTPEQFEREYAEREGRP
                     RRPSTASPG"
BASE COUNT          243 a          633 c          679 g          272 t
ORIGIN      
        1 aagaagcgat cgcgtgggca agcctgccgg tcgcagcagg gcaggtcgca ctcgagatcg
       61 gtgctgcacc tggcggtgcc gtgctcgcgc tcgcgcgccg cgggctggag gtgtgggcgg
      121 tcgataccgg tgcgcttgcc ccgcaggtcg ccgccttgcc ttccgttcac cacctcgcca
      181 agaaggtggg cgcgctgcgc tgggaggagc tccctgcgca gatcgactgg ctcctggtcg
      241 acgtcaacct cgcaccccag gtcgcgctgc acgaggttcg acggctgatg ccccggctcg
      301 tgggcacgct gcgcggagcg gtgttcacgc tcaagctcaa cgactgggcg ttcgtggcgg
      361 agctgccggc gctggtcgat cgcatccggc agatgggtct ggacgacgtc cggctgcggc
      421 atctgccgtc gaaccggcgc gaggtttgcg ccgtcgcgca acacgcgagc gcgcgattgt
      481 agggtcgccc tggtcccgcc gatgtccacc tggacaatcc gggtgcgatc cgggcggttc
      541 gacgcgttca tagatgagac ggcctcacgt ccagcgcgtc gtaaacacgc gagatgctcg
      601 caaccgccgc ggctgtgtgc aacgagcgtt cgcgtctggt cgcaggcacg agcgatgcag
      661 tcggaccgta accgacccag cggtgattgg tcttcgtgcc agggggcgcg tcgccggtcg
      721 ccgcgatgcc ccggagaacc ccgtgaccga ccacgatcac acctcgcaca gctactccgt
      781 cgagatctcc gcgcttgtcc gggtgctcgg cgaccgggtc cgccagcagg tctgccagct
      841 gcgcagcatc tcgctcggcg gcacgttcat cgaagtcggc cgcttgccga tgggcacgca
      901 ggtcaacatc acgttcgggt tgccgacgat cggcgagcgg atctcgctcg acgccgtcgt
      961 gcactggagc acggactacg gcgtcggcgt gcagttcgag ggcatgcggg cgcgccaggt
     1021 gtgggcgctg tggcgctacc tggagtcggt gaccggcgcc gagcccgagg cctggctcgg
     1081 cgacaccaag gagatcatca tgaattgatc gcgggcgacg cgacgtcccc tgttcgatcg
     1141 acctagcccg gggaggcggt gctcgggcgg cggggccggc cctcgcgctc ggcgtactcg
     1201 cgctcgaact gctcgggcgt gtagcgggtg ccgagcggcg gcggcggatc gctctggatg
     1261 aacgcgttgt cccggatccg gagcgcccgg atccgggcga gcacggcgtc gggctgggtc
     1321 gcgcccgcgc tgcgcgcgag gatctcgacc gcggcgtcga gcgcgcgcgg cgacaccggg
     1381 atgccgccct cgtcgtcgcg ccggacctcg caggcggcga gcgcggcatg cgcgatgccc
     1441 cacgacggcg acgacaccgc ggcgatcagc gagtcgaact tgccggcctc gagctcgtcg
     1501 cacgcctggc gctccagcga cagctccgcg ctggcgcgca ccggcggcac ctcctgcgcg
     1561 acgatccggt cgagcgtctc ggcgagctcc gcggcgtcgc ctggctcgat cgcatcgccc
     1621 tgcgcgagct cgacggcggt cttggcgtag cgcagcgcat cgacgtggcg gccctggccg
     1681 gcgagcgcgc gggccatggc gtgcagcccg gcgagccggg tggcgcggtc ggcgtggccg
     1741 atcagctgct gagcgaccgg ctcgcaggcg acgaactcgc cgatccggta caggctggtc
     1801 gcgagcacgg cgagcaccgc cggatcc
//
LOCUS       DASVOR010001953         2831 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_313219,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001953 DASVOR010000000
VERSION     DASVOR010001953.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313219"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VF516_36275"
     CDS             <1..493
                     /locus_tag="VF516_36275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8113261.1"
                     /translation="LVNNAGVAINGLVMRFSDEHWQKTLAVNLGGAFHCIRAASSLLL
                     RAKAAGRIINLTSVIGEMGNGGQTAYAASKAGLIGLTMSTARELASRGITCNAIAPGF
                     IETDMTAEHLPEAQRGKLLEQIPLGKIGRPEDVADAAAFLAGPEAAYITGQVLRVNGG
                     LLM"
     gene            545..787
                     /locus_tag="VF516_36280"
     CDS             545..787
                     /locus_tag="VF516_36280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8113262.1"
                     /translation="MSPEEIEPKVKEIICNQLEVSSEQLRPEASFIDDLKADSLAVVE
                     LVLAFEQEFKITIPEEDTEQIKTVKDAINYIKSHAK"
     gene            850..2109
                     /gene="fabF"
                     /locus_tag="VF516_36285"
     CDS             850..2109
                     /gene="fabF"
                     /locus_tag="VF516_36285"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEX8113263.1"
                     /translation="MDSRRRVVITGIGLITPIGNDLEATWSGLMAGRSGAGPITLFDS
                     TNFATRFACEVKGWDPAPFFDKRELKHLDRFLQFGVAAGMMAVADAGLPPRLPEAEAD
                     RWGVFIGAGLGGVHTIEHTYAAQAAKGPRHGFSPYFVTDIIINEVPGMISIRTGATGP
                     NMSHVSACSTGAHSIGEAMRTIRHGYADGMIAGGTEATIAILGVGGFNAMRAMSTRNA
                     EPQRASRPFDKDRDGFVIGEGAGVVVLEELDHARRRGARIYAELIGYAANSDAHHVAA
                     PAPDHRGAQACFRTALKDARLPPGAIGYINAHGTSTELNDKNETIAVKAVFGDHARKL
                     AMSSTKSMTGHTLGAAGGIEAAITALAVARGVLPPTTNYDTPDPDCDLDYVPNQPREQ
                     RVDAAMSNSFGFGGTNAALVVTRYKGD"
     gene            2114..2647
                     /gene="nrdR"
                     /locus_tag="VF516_36290"
     CDS             2114..2647
                     /gene="nrdR"
                     /locus_tag="VF516_36290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEX8113264.1"
                     /translation="MRCPFCGGSEDKVIETRVSKDGGEIRRRRECDSCARRYTTYERI
                     EESMPLVIKNDGRREPFDRAKIEHGLLAAVAKRPVSRDQVAALALEVEREIAELGVSE
                     IASRDIGERVLPRLRTLDQVAYVRFASIYRDFRDLEGFEKELDALRNDTGPHLLSDEH
                     TDKHAMVVPADPAERTG"
BASE COUNT          470 a          995 c          999 g          367 t
ORIGIN      
        1 cctggtcaac aacgccgggg tcgcgatcaa cggcctggtg atgcggttct ccgacgagca
       61 ctggcagaag acgctcgcgg tcaacctcgg cggcgcgttc cactgcatcc gcgcggcgtc
      121 gagcctcctt ttgcgcgcca aggccgccgg ccggatcatc aacctcacgt cggtgatcgg
      181 cgagatgggc aacgggggcc agaccgccta cgcggcgtcc aaggccggcc tgatcgggct
      241 gacgatgtcg accgcccggg agctcgcgtc gcgcggcatc acctgcaacg cgatcgcgcc
      301 ggggttcatc gagaccgaca tgaccgccga gcacctgccc gaggcccagc ggggcaagct
      361 gctggagcag attccgctgg gcaagatcgg ccggcccgag gatgtcgcag acgccgcggc
      421 gtttctggcc ggaccggagg cagcgtacat caccggccag gtgctgaggg tcaacggcgg
      481 gctgttgatg tagcgcccga tctggactat caacccggca acccggggca acccacgagg
      541 aaccatgtcg cctgaagaga tcgaacccaa ggtcaaggaa atcatctgca accagctgga
      601 ggtgtcctcc gagcagctgc ggcccgaggc gtcgttcatc gacgatctca aggcggactc
      661 gctcgccgtg gtcgagctcg tgctcgcgtt cgagcaggag ttcaagatca cgatccccga
      721 ggaagacacc gagcagatca agaccgtcaa ggacgcgatc aactacatca agagccacgc
      781 gaagtaaccg tggcgcgggc ggtcggcccc ccgcctgcgc acggggccaa gctgcggtaa
      841 ggagccgtca tggactctcg acgtcgcgtc gtcatcaccg gcatcggcct catcaccccg
      901 atcggcaacg acctggaagc gacgtggtcg ggcctgatgg cgggcaggag cggggccggg
      961 ccgatcacgc tgttcgactc cacgaacttc gccaccaggt tcgcctgcga ggtcaagggc
     1021 tgggaccccg cgccgttctt cgacaagcgc gagctcaagc acctcgaccg cttcctgcag
     1081 ttcggcgtcg cggccggcat gatggcggtc gcggacgcgg ggcttccgcc gcggctgccc
     1141 gaggccgagg ccgaccgctg gggggtgttc atcggcgcgg ggctcggcgg ggtccacacc
     1201 atcgagcaca cgtacgccgc gcaggccgcc aaggggccgc gccacgggtt ctcgccgtac
     1261 ttcgtcaccg acatcatcat caacgaggtc ccgggcatga tctcgatccg gaccggcgcc
     1321 accgggccga acatgtcgca cgtctcggcg tgctcgaccg gcgcgcactc gatcggcgag
     1381 gcgatgcgca cgatccgcca cggctacgcc gacggcatga tcgcgggcgg caccgaggcg
     1441 acgatcgcga tcctcggggt cggcgggttc aacgcgatgc gcgcgatgtc gacccgcaac
     1501 gccgagcccc agcgcgcgag ccggccgttc gacaaggacc gcgacggctt cgtgatcggc
     1561 gagggcgccg gcgtggtcgt gctcgaggag ctcgatcacg cccggcggcg cggcgcgcgg
     1621 atctacgccg agctgatcgg ctacgccgcc aacagcgacg cgcaccacgt cgcggcgccg
     1681 gcgccggacc accgcggcgc ccaggcgtgc ttccgcaccg cgctcaagga cgcgcggctt
     1741 ccgccgggcg cgatcgggta catcaacgcg cacggcacgt cgaccgagct caacgacaag
     1801 aacgagacca tcgcggtcaa ggcggtgttc ggtgatcacg cccggaagct cgcgatgtcg
     1861 tcgaccaagt cgatgaccgg ccacacgctc ggcgcggccg gcgggatcga ggccgcgatc
     1921 accgcgctcg cggtcgcgcg cggcgtgctg ccgccgacca ccaactacga cacgccggat
     1981 cccgactgcg atctcgacta cgtgcccaac cagccgcgcg agcagcgggt cgacgccgcg
     2041 atgtcgaaca gcttcgggtt cggcgggacc aacgcggcgc tggtcgtgac ccgctacaag
     2101 ggcgactagc gacatgcggt gcccgttctg cggcggctcg gaggacaagg tgatcgagac
     2161 ccgggtgtcg aaggacgggg gcgagatccg ccgccgccgg gaatgcgaca gctgcgcgcg
     2221 tcgctacacg acctacgagc ggatcgagga gtcgatgccg ctggtgatca agaacgacgg
     2281 ccgccgcgag ccgttcgacc gcgccaagat cgagcacggg ctgctcgccg cggtcgccaa
     2341 gcgcccggtg tcgcgcgatc aggtcgccgc gctggcgctc gaggtcgagc gcgagatcgc
     2401 cgagctcggg gtgtccgaga tcgcctcgcg cgacatcggc gagcgcgtgc tgccgcggct
     2461 gcgcacgctc gaccaggtcg cctacgtccg gttcgcgtcg atctaccgcg acttccgcga
     2521 tctcgagggc ttcgagaagg agctcgacgc gctccgcaac gacaccggtc cgcacctcct
     2581 gtccgacgag cacaccgaca agcacgcgat ggtcgtcccc gcggacccgg cggagcgcac
     2641 cgggtgagtc gacgggtcgg ggcggcgcgc ggaccggacc gggcggcgca gcaggacgtg
     2701 gatcggggtc acatggcgcg ctgcctggag ctcgccgagg cgtaccgcgg ccggaccgcg
     2761 cccaacccga tcgtcggctg cgtcatcgtc gaccgcgccg gccgggtgat cgccgagggc
     2821 gcccatgccg g
//
LOCUS       DASVOR010001954         5071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_137531,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001954 DASVOR010000000
VERSION     DASVOR010001954.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137531"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(303..500)
                     /locus_tag="VF516_36295"
     CDS             complement(303..500)
                     /locus_tag="VF516_36295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX8113265.1"
                     /translation="MDLPLSERAELAADLLASLDGEAEADVEAAWASEIERRAREALA
                     NPDDDVAWETLRAELHAAPTR"
     gene            complement(744..1061)
                     /locus_tag="VF516_36300"
     CDS             complement(744..1061)
                     /locus_tag="VF516_36300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113266.1"
                     /translation="MERGWRATEPVGKTAEPWRHGSHASAIRSPDEPDGGRRGGRRGP
                     GVQLSNCEPKQTAICYQYVDAEGPHRLQCAPTMAICEAAKRLQKHTPDGARLGYTECH
                     PID"
     gene            complement(1100..1360)
                     /locus_tag="VF516_36305"
     CDS             complement(1100..1360)
                     /locus_tag="VF516_36305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113267.1"
                     /translation="MVAFRDAAGNPLRVFYALDTKLPLGAELVEAGTDPPVLIRVTVD
                     DWRWLGPVRAFHRALYEHHGQRYEYAYTQIRFNDVEPGELAR"
     gene            1656..1859
                     /locus_tag="VF516_36310"
     CDS             1656..1859
                     /locus_tag="VF516_36310"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8113268.1"
                     /translation="MSDRTNPRTGSDPVYVDEQELSRLTTLSRTTLSTMRRKGGGPKF
                     AKLGHRIVYRLADVERWIEEHTR"
     gene            1856..2092
                     /locus_tag="VF516_36315"
     CDS             1856..2092
                     /locus_tag="VF516_36315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8113269.1"
                     /translation="MTPGQSQPGQRTPAVPSDPIYVDDRELARRTPIRRITWQTWRRQ
                     GAGPPFYKLGRRCVYRWAEVVAWLESHRVGGAGP"
     gene            complement(2411..2848)
                     /locus_tag="VF516_36320"
     CDS             complement(2411..2848)
                     /locus_tag="VF516_36320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113270.1"
                     /translation="MPADAGRRPPHRTPPRPDRIRRIAGGFAFVPNDVLHRGFFASLS
                     HTERSLYLFLVLAGDRNGVSFYAYDRICDALEFTQDEYLVVRKSLIDKDLIAFDGRRF
                     QVLSLPPAPILPARRPLVTQDDFDDHDPATIQHIIRSSLDRRR"
     gene            complement(2911..3753)
                     /gene="istB"
                     /locus_tag="VF516_36325"
     CDS             complement(2911..3753)
                     /gene="istB"
                     /locus_tag="VF516_36325"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX8113271.1"
                     /translation="MPKPPRIRRRAKPQARGDGGVLERLRDHLERLGLKELDEHLDAH
                     LAWAHEHNPPPLMLLERVLGDGAQRLRQRRVERRIDTCGLRARKTLAAFDWAFQPSLN
                     RAFIENLATLAFIDNHEDLLITGKNGTGKSHILQALTLRACEREIRVRYTRCVDLIDD
                     LYAGLADQTYDTRLRKWARAEWLVIDDVGLGQLRRRDDEPTAAHMLFNLLDSRHEHAP
                     TALTSNIKLGQWGKYLGDATLAAAILDRLAMTSIRVDIDGPSYRDELARRRAKDHGVV
                     DDST"
     gene            complement(3651..>5071)
                     /gene="istA"
                     /locus_tag="VF516_36330"
     CDS             complement(3651..>5071)
                     /gene="istA"
                     /locus_tag="VF516_36330"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX8113272.1"
                     /translation="PDITARRVYEELCGRVEPVFEGRYTIVKALVRKLRPKPVVELST
                     PMEEPEPGKVAECDWAMISLDFKNGTRRKLQIFGYTLAYSHRRYYRFYDRADFHALLD
                     GHVEAFDHADGLAATCKYDGQKTVVLRWEGPQPIYNPRFVDFATHYRFGPQACRPGHP
                     NDKPHVELSFRSLRISFFNGRDFHDLDDLKVQLARWSTNIDDERPQRRKQRRTPLELH
                     AEERPHLIARPRHAYDTARVAYRVCDLEGFVAWDGNRYSLPAENVTELLPVRITQREI
                     FVYGQDLRLVAQHELRPRGAGELVIAPGHRPKSDRGPGLDQLRVVYRELGDGADQFLA
                     ELERALPRSAGYHARQILAFRERYQTGDVIAAMAHAQQYGAFEYHAIERILAARAAPR
                     RLDEYVAEATEHKLAAIVRQSRTEPRALAMYDALPNWSRRAPRGDAACPSHPASDAEP
                     SRKPEETAESSSDSATTSSDSD"
BASE COUNT          754 a         1622 c         1867 g          828 t
ORIGIN      
        1 gggcatcatc gcggtcgtgg gctcggcgcc cggctgggag gatccgtggt ctctcgcgcg
       61 tcgcctcgat gttcttcgcg gcgcgagcga gtacgagctg atgctgttcg acgagctcgg
      121 cggatacgtg gatgaccgcg atgtccagct ggcgcgcctc gaagccgtgg ttcactgagc
      181 gggatctcca tcgcctgcgt cgagatcggc gacagagacc tggcctggca cctcgccgtc
      241 agcccgccgg cctccgtgat cgagcaccgg cggggcgggg cgggcaatcc ggaccggctt
      301 gctcatcgcg tcggagctgc atgcagctcg gcgcgtaacg tttcccaggc gacgtcgtcg
      361 tcgggattcg ccagcgcctc acgcgcgcgc cgctcgatct cgctggccca cgctgcttcc
      421 acgtcggcct cggcctcgcc gtccaggctg gcgagcagat ccgccgccag ctcggcgcgc
      481 tcggacagcg gcaggtccat cgcatcgtgc agcagcttgc cggctcgctc ggacatcgcg
      541 ctcatgacag caaatcgggg cggaagcgga accgtggcgt gcacgaccgc gatgtcgagt
      601 tggacatgat gacgacctcg gaggcgtggc gggtgggtgc cgatcgcggc tgggcgttcc
      661 cgaccgatgc gctcgaggag cggccgtgcg acctctgcgc cgagctgggc cagccggcgc
      721 atgtcgccgg cgcgcccggg cggtcaatcg atgggatggc attcggtgta tccgagccgc
      781 gcgccgtccg gtgtgtgttt ctgcaggcgc ttggcggcct cgcagatcgc catcgtcggc
      841 gcgcactgca gacggtgcgg accctccgcg tcgacgtact gatagcagat cgccgtctgc
      901 ttgggctcac agttcgagag ctgaacgccg gggccgcgac gaccgccgcg acgaccacca
      961 tccggttcat ccggcgagcg tatcgcagat gcgtgcgatc cgtgtcgcca tggctccgcg
     1021 gtcttgccca ccggctcggt cgcgcgccag ccgcgctcca cgcctcggct caaccactgg
     1081 ctccggagcg gctccgccgt catcgcgcta gctcgccggg ctcgacgtcg ttgaaccgga
     1141 tctgcgtgta ggcgtactcg tagcgctggc cgtggtgttc gtagagtgcg cggtggaacg
     1201 cgcgcaccgg gccgagccag cgccagtcgt cgacggtgac ccggatcagc acgggcggat
     1261 cggtgcccgc ctcgacgagc tcggcgccga gagggagctt ggtgtcgagc gcgtagaaca
     1321 cgcgcagcgg attgccggcg gcgtcgcgga atgcgaccac tcgcgcgcgg tggacagcgc
     1381 cgcagccctg gttctaaaac catcgcaaat ccaaatattt gcacaaatcg ccccctagga
     1441 aaggcgatgg cctactggaa ggcgtgcgga actctaccga gtcgttgacc acttcaggcc
     1501 cgctcgacgc ccacgcgcgc gagcaacccg gaacaaacac gctgcggccc gaagcgtgtg
     1561 cttcgtcgac ggtgtcacgc cacggaccac gaggagacaa cccgagcact actgaatttc
     1621 gaccggggtt gacccacggt ggtctgctcg tgggcgtgag tgaccgaacc aacccgcgga
     1681 ccggctctga tcccgtgtat gtcgacgagc aggagctctc acgcctcacg acgctgtcgc
     1741 ggacgacgtt gtcgacgatg cgcaggaagg gtggaggccc gaagttcgcc aagctcggcc
     1801 accgcatcgt ctaccggctc gcggacgtgg agcgctggat cgaggagcac acgcgatgac
     1861 ccccggtcag tcccagccgg gacagcgcac accagcagtg ccgagcgatc cgatctacgt
     1921 cgatgaccgc gagctggcgc gccgcacgcc gatccgccgc atcacgtggc agacgtggcg
     1981 ccgacagggc gcgggaccgc cgttctacaa gctcgggcgg cgctgcgtgt accgctgggc
     2041 cgaggtggtc gcgtggctcg agtcgcaccg agtcggcgga gccgggccgt gagctccgca
     2101 gccgagcttg cccgcgcagc tctgcggccc gggcgcgatc ctggacctta ggtgccggtc
     2161 aggattggat ccaatcacgg cggtggatcg gatccagttc ggttgctgtg gacccgctgc
     2221 gcgacgtcgg tgcggatcgg tctggatccc tgagcgggtg tagcccgttc gtctggatcc
     2281 tggtgatgga tcggctggat ggtctggatc gatcgggggc gtacgcgacg cgcggacgtg
     2341 gcgtcagcga cgacgatcga gaggatggtc tggatcgatc ggggggcgac gcgcgacgcg
     2401 cgacgtggcg tcagcggcgg cgatcgagag acgaccggat gatgtgctgg atggtggcgg
     2461 ggtcgtggtc gtcgaagtcg tcctgggtga ccaggggccg ccgggccggc aggatgggcg
     2521 ccggcggcag cgacaggacc tgaaatcggc ggccgtcgaa ggcgatgaga tccttgtcga
     2581 tgaggctctt gcgcaccacg agatactcgt cctgcgtgaa ctcgagggcg tcgcagatcc
     2641 ggtcgtaggc gtagaagctg acgccgttgc gatcgccggc gaggacgagg aagaggtaga
     2701 gcgagcgctc ggtgtggctc agcgacgcga agaagcctcg gtgcaggacg tcgttgggga
     2761 cgaaggcaaa gccacccgcg atgcggcgga tccgatcggg ccgcggcggg gtccggtgcg
     2821 gcggccgtcg gccagcgtcg gccggcatgt cagcccgcct cggcggcgca gcgtggcgcg
     2881 ggcagccagg cgcgcggctc tgagcggcgg ctaggtggag tcgtcgacaa cgccgtggtc
     2941 cttggcgcgc cgccgggcga gctcgtcgcg gtagctcggg ccgtcgatgt cgacgcggat
     3001 cgaggtcatg gcgagccggt cgaggatcgc cgcggccagg gtggcatccc cgaggtactt
     3061 gccccactgg ccgagcttga tattcgacgt gagggcggtc ggggcatgct catgccggct
     3121 gtcgagcagg ttgaagagca tgtgcgcggc ggtgggttcg tcgtcgcggc ggcgcagctg
     3181 gccgagcccg acgtcgtcga tgaccagcca ctccgcgcgc gcccattttc ggagccgggt
     3241 gtcgtaggtc tgatcggcga ggccggcata caggtcgtcg atcaggtcga cgcaccgggt
     3301 gtagcggacg cggatctcgc gctcgcacgc ccgcagcgtg agcgcctgca ggatgtggct
     3361 cttgccggtc ccgttcttgc cggtgatcag cagatcttcg tggttgtcga taaacgcgag
     3421 cgtggcgagg ttctcgatga aggcgcggtt gaggctgggc tggaaggccc agtcgaaggc
     3481 cgcgagggtc tttcgggccc gcaggccgca ggtgtcgatc cggcgctcga cgcggcgctg
     3541 ccggaggcgt tgcgcgccat cgccgagcac gcgctcgagg agcatgagcg ggggtgggtt
     3601 gtgctcgtgg gcccacgcga ggtgggcgtc gaggtgctcg tcgagttctt tcagtccgag
     3661 tcgctcgagg tggtcgcgga gtcgctcgag gactccgccg tctcctcggg cttgcggctt
     3721 ggctcggcgt cggatgcggg gtggcttggg catgcggcat ctcctcgggg cgcacgtcgc
     3781 gaccagttgg gcaaggcgtc gtacatcgcc agcgcgcgcg gctcggtgcg gctctggcgc
     3841 acgatcgccg cgagcttgtg ctcggtcgcc tcggcgacgt actcgtcgag ccgacgcggc
     3901 gccgcgcggg ccgcgaggat gcgctcgatc gcgtggtact cgaaggcgcc gtactgctgg
     3961 gcgtgcgcca tcgccgcgat cacgtcgccg gtctggtagc gctcgcgaaa cgccaggatc
     4021 tggcgtgcgt ggtagccggc cgagcgcggc agtgcacgct cgagctcggc caggaactgg
     4081 tcggcgccat cgccgagctc gcggtacacg acgcgcagct gatcgaggcc cggcccgcgg
     4141 tccgacttgg gacgatggcc gggcgcgatc acgagctcgc cggcgccgcg cggccggagc
     4201 tcgtgctgcg cgacgagcct caggtcctgg ccgtacacga agatctcacg ctgcgtgatc
     4261 cggaccggca ggagctcggt gacgttctcg gccggcagcg agtagcggtt gccatcccat
     4321 gccacgaagc cctcgaggtc gcacacccgg tacgccaccc gcgcggtgtc gtaggcatgg
     4381 cgcggccggg cgatcaggtg gggccgctcc tcggcatgaa gctcgagcgg ggtccggcgt
     4441 tgcttcctgc gctggggccg ctcgtcgtcg atgttggtcg accagcgcgc gagctggacc
     4501 ttgaggtcgt cgaggtcgtg aaagtcgcgg ccgttgaaga agctgattcg caaggagcga
     4561 aagctcagct ccacatgagg cttgtcgttg gggtgccccg gccggcacgc ctggggtcca
     4621 aatcgatagt gcgtcgcgaa atcaacaaag cgtggattgt agatgggctg tggaccttcc
     4681 cagcgcagca ccacggtctt ctgtccatcg tacttgcacg tcgcagccag gccatcggca
     4741 tggtcgaagg cctcgacgtg accatcgagc agcgcatgga agtcagcccg gtcgtaaaat
     4801 cgataatagc gtcgatggct ataggcgagc gtatagccga agatctgcag tttgcgccgt
     4861 gtaccattct tgaaatcgag cgagatcatc gcccagtcgc actcggccac cttgccgggc
     4921 tcgggctcct ccatcggcgt cgacagctcg accactggct tgggccgcag cttgcgcacc
     4981 agagccttga cgatcgtgta gcggccctcg aagaccggct ctacgcggcc acacagctcc
     5041 tcgtagaccc gccgcgccgt gatgtcggga a
//
LOCUS       DASVOR010001955         2566 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_166546,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001955 DASVOR010000000
VERSION     DASVOR010001955.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166546"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VF516_36335"
     CDS             <1..778
                     /locus_tag="VF516_36335"
                     /inference="COORDINATES: protein motif:HMM:NF025167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HEX8113273.1"
                     /translation="ELSDGAYWATPVAASCSHAPRSLDVGYALLFDLDSLHRGLLHVA
                     GQTAILRDARPVQVALDQATSVASFIKEGVWHIWMGIDHILFLTCLILPAVFQRRTQR
                     WVAADSLRDVCREVFEIVTAFTLAHSITLVISAVGLVALPSRWIETAIALSVVAAALN
                     NLLRTVDARWAVAFALGLLHGFGFSSVLIDLGLPSHELIGALLGFNLGVELGQAAIVV
                     ALLPALYWIRRTVAYQALLWAGSAGVALIATLWSYQRWFA"
     gene            complement(795..>2566)
                     /locus_tag="VF516_36340"
     CDS             complement(795..>2566)
                     /locus_tag="VF516_36340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8113274.1"
                     /translation="HTLIRDGRTLHLPSNELAPELARALLARAQADALLIVPLRSAGT
                     PRGGLLMIARGRELEEDDWRAFGLGIATQVSHVVTLARAYEERELAERRATEHAALLD
                     AMIASAPDYVAHLDLDGTIRLMNRPLFGHPATSVVGTNIFRYPGGDHAIALRQALARI
                     EQTGEPQGFEASVFRPDGSQVWYSTRLGPVKEHGKVTGAVLVSRDVSDQKQTEMHLMV
                     SDRMASVGTLAAGVAHEINNPLASVIANLDMALDDIAGLAETTRLPPDLSEELKDARS
                     AADRVREIVRDLKIFSRGEEDRHGPVDVEDVLESTVRMARNELRHRARLVKNYGKVPP
                     VDAHESRLGQVFLNLIVNAAHAIPPGNHEANEIRLSTAVDAAGRIVVSIRDTGTGIPP
                     EVRPRLFTPFFSTKPVGVGTGLGLAISHRIITQLGGTITYDTEVGKGTEFHIALPVAG
                     GPVAPQPPPEIGTRAPAVRRGNLLVIDDEESLAQAIRRYLGAEHDVTAVYRARDALDL
                     LERGTRYDVILCDLMMPQITGMELHAEVLRIDPAQASRIVFLTGGAFAPSAREFLATT
                     TNRRIEKPFDLKEVRRLVNEFIR"
BASE COUNT          310 a          887 c          957 g          412 t
ORIGIN      
        1 cgagctgtcg gacggcgcgt actgggcgac gccggtggcg gcgagctgca gccatgcgcc
       61 gcggtcgctg gacgtcggct acgcgctctt gttcgacctc gactcgctgc accgcggcct
      121 gctccacgtc gccgggcaga ccgcgatcct gcgcgacgcg cggccggtgc aggtcgcgct
      181 cgaccaggcg acgtcggtcg cctccttcat caaggaaggc gtgtggcaca tctggatggg
      241 catcgaccac atcctgttcc tgacctgcct gatcctgccc gcggtgttcc agcgccggac
      301 ccagcgctgg gtcgccgccg actcgctgcg cgatgtgtgc cgggaagtgt tcgagatcgt
      361 caccgcgttc acgctcgcgc actcgatcac gctggtgatc tcggcggtcg gactggtcgc
      421 gctgccgtcg cggtggatcg agaccgcgat cgcgctgtcg gtggtcgccg ccgcgctcaa
      481 caacctgctg cgcacggtcg acgcccggtg ggcggtcgcg ttcgcgctcg gcctgctcca
      541 cggcttcggg ttctccagcg tgctgatcga cctcgggctg ccgtcgcacg agctgatcgg
      601 cgcgctgctc gggttcaacc tcggggtcga gctcggccag gccgcgatcg tcgtcgcgct
      661 cttgcccgcg ctgtactgga tccgccgcac ggtcgcctac caggcgctgc tgtgggccgg
      721 ctcggccggc gtcgcgctga tcgcgaccct gtggtcgtac cagcgctggt tcgcgtgacc
      781 gcggtcagcg ccggtcagcg gatgaactcg ttgaccagcc ggcggacttc tttgaggtca
      841 aacggtttct cgatccgccg gttggtcgtg gtcgccagga actcgcgcgc cgacggggcg
      901 aacgcgccgc cggtcaggaa cacgatgcgc gacgcctgcg cggggtcgat ccgcagcacc
      961 tcggcgtgca gctccatgcc ggtgatctgc ggcatcatca gatcgcacag gatcacgtcg
     1021 tagcgggtgc cgcgctcgag caggtcgagc gcgtcgcgcg cccggtacac cgcggtgacg
     1081 tcgtgctcgg cgccgaggta ccgccggatc gcctgggcga ggctctcctc gtcgtcgatc
     1141 accagcaggt tgccgcggcg caccgccggc gcgcgggtcc cgatctccgg gggcggctgg
     1201 ggcgcgaccg ggccgccggc gaccggcagc gcgatgtgga actcggtgcc cttgccgacc
     1261 tcggtgtcgt aggtgatcgt gccgccgagc tgggtgatga tgcggtgcga gatcgccagg
     1321 ccgaggccgg tgccgacgcc gaccggcttg gtgctgaaga acggcgtgaa cagacgtggc
     1381 cggacctcgg gcgggatccc ggtgccggtg tcgcggatcg agaccacgat gcggcccgcg
     1441 gcgtcgaccg cggtcgacag ccggatctcg ttggcctcgt ggttgccggg cgggatcgcg
     1501 tgggcggcgt tgacgatcag gttgaggaac acctggccga gccgcgactc gtgggcgtcg
     1561 accggcggca ccttgccgta gttcttgacc agcctggcgc ggtggcggag ctcgttccgc
     1621 gccatccgca cggtcgactc cagcacgtcc tcgacgtcga ccgggccgtg gcggtcctcc
     1681 tcgccgcgcg agaagatctt gaggtcgcgc acgatctcgc gcacccggtc ggcggcgctc
     1741 cgcgcgtcct tgagctcctc ggacaggtcg ggcggcagcc gggtggtctc ggcgagcccg
     1801 gcgatgtcgt ccagcgccat gtcgaggttg gcgatcaccg acgcgagcgg gttgttgatc
     1861 tcgtgggcga cgccggccgc cagcgtgccg accgacgcca tgcgatcgga caccatcagg
     1921 tgcatctcgg tctgcttctg gtcgctgacg tcgcgcgaca ccaggaccgc gccggtgacc
     1981 ttgccgtgct ccttgaccgg cccgagccgg gtcgagtacc agacctggct gccgtcgggg
     2041 cggaacaccg aggcctcgaa gccctggggc tcgccggtct gctcgatccg ggccagcgcc
     2101 tggcgcagcg cgatcgcgtg gtcgccgccc ggatagcgga agatgttggt gccgaccacg
     2161 ctggtcgccg ggtgcccgaa cagcggccgg ttcatcagcc ggatcgtgcc gtcgagatcg
     2221 aggtgggcga catagtccgg ggcgctggcg atcatcgcgt cgagcagcgc ggcgtgctcg
     2281 gtggcgcggc gctcggcgag ctcgcgctcc tcgtaggccc gggcgagcgt gaccacgtgg
     2341 ctgacctggg tcgcgatgcc caggccgaac gcgcgccagt cgtcctcctc gagctcgcgg
     2401 ccgcgcgcga tcatcagcaa gccgccgcgc ggcgtgccgg cgctgcgcag cgggacgatc
     2461 agcagggcgt cggcctgggc gcgggccagc agcgcgcgcg cgagctcggg agccagctcg
     2521 ttcgacggca ggtgcagggt ccggccgtcg cggattagcg tgtgga
//
LOCUS       DASVOR010001956         2805 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_458892,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001956 DASVOR010000000
VERSION     DASVOR010001956.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458892"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VF516_36345"
     CDS             <1..1239
                     /locus_tag="VF516_36345"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113275.1"
                     /translation="HLAEDDDFIQMLVDEAKIASVLRHINIARVYEFARAHGEYFIAM
                     EHVDGKDMRTVLERCRTKKKPIPPEHAAYVAAEVGSALHAAHSAIDARGRPLRIIHRD
                     VSPSNIICSYAGEVKLCDFGIAKATLSRVTTKTGVIKGKVKYMSPEQALGRKLDHRSD
                     VFSLGSCLYEMLTRIPPFTATNEMDLLIKVRDAKYRPISELTPGIPPELEAIADKCLT
                     RSRANRYQTAAEAETDLRNFLRKFMPNYSRSHLGRFIRKMFAAEIERELRMLEEYVLD
                     EDVSDDLGESMIARDDDFEPPEVPFQPKPTSVSILAIPEEASDFSRDSNHTTAPKSDA
                     ADIHGANTMILDNGRGRGASRPPPPPPPAHSRAQTQPPPVPAPRPRPTPPSRSEFDED
                     LHSAPTMIIDVGRMRDRGRN"
     gene            complement(1268..2254)
                     /locus_tag="VF516_36350"
     CDS             complement(1268..2254)
                     /locus_tag="VF516_36350"
                     /inference="COORDINATES: protein motif:HMM:NF019127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HEX8113276.1"
                     /translation="MKYYIVIPLLLAPLAGACTPESMAAPDPNENAPTSTAGSSSQMP
                     IKQVEQIVGAQGEVTNGVLNITIMRTDIGEVHGPLGVTFTPAFEVHGELYFQPLGKNQ
                     ALLNADMAVLEPETNPFIAALLQHGLVFQAFHQHLPMLPQVWFIHFRGIGDPIALARA
                     TRAALEVTHVRLPQPPPPPHPTTPLDAHRLAHILHGEAHVGAEGVVTVVVARTDRVVL
                     EGVRVSPYAGISTTIQFKPIRRDDDAGMANESKPGGKANAQVCPDFSMTAAEVVPVVK
                     RMQIEHKFFQGSLRNDETNEQPQLFWDAMVKEGDAYQLAEEIRSALNLTKCK"
BASE COUNT          462 a         1012 c          907 g          424 t
ORIGIN      
        1 cacctcgccg aggacgacga cttcatccag atgctggtcg acgaggccaa gatcgccagc
       61 gtcctgcgcc acatcaacat cgcccgggtc tacgagttcg cgcgcgcgca cggcgagtac
      121 ttcatcgcga tggagcacgt cgacggcaag gacatgcgca ccgtgctcga gcgctgccgc
      181 accaagaaga agccgatccc gcccgagcac gcggcctacg tcgccgccga ggtcggctcg
      241 gcgctccacg ccgcgcactc ggcgatcgac gcgcgcggcc ggccgctgcg gatcatccac
      301 cgcgacgtct cgccgtcaaa catcatctgc agctacgcgg gcgaggttaa gctgtgcgac
      361 ttcggcatcg ccaaggccac gctgtcgcgc gtgaccacca agaccggcgt gatcaagggc
      421 aaggtcaagt acatgagccc cgagcaggcg ctcggccgca agctcgacca ccgcagcgac
      481 gtgttctcgc tcggctcgtg cctgtacgag atgctgaccc ggatcccgcc gttcaccgcg
      541 accaacgaga tggacctgct gatcaaggtg cgcgacgcca agtaccggcc gatctccgag
      601 ctgaccccgg gcatcccgcc cgagctcgag gcgatcgccg acaagtgcct gacccgcagc
      661 cgcgccaacc gctaccagac cgcggccgag gccgagactg atctgcggaa cttcctgcgc
      721 aagttcatgc cgaactactc gcgcagccac ctcggccggt tcatccgcaa gatgttcgcc
      781 gccgagatcg agcgcgagct ccgcatgctg gaggagtacg tgctcgacga ggacgtcagc
      841 gacgacctcg gcgagagcat gatcgcgcgc gacgacgact tcgagccgcc cgaggtgccg
      901 ttccagccca agccgacctc ggtctcgatc ctcgcgatcc ccgaggaggc gagcgacttc
      961 tcgcgcgact ccaaccacac caccgctccc aagtcggacg ccgcggacat ccacggcgcc
     1021 aacacgatga tcctggacaa cgggcgaggc cgcggtgcgt cccggccacc accaccacca
     1081 ccaccagcac actcacgcgc acagacccag ccaccgccgg tacctgcgcc gcggccccgc
     1141 ccgacgccac cttcgcgctc cgagttcgac gaggatctgc actcggcccc gacgatgatc
     1201 atcgacgtcg gccggatgcg cgaccgcggg cgaaactagc gccgcggtcg ccctcgccgg
     1261 tgcggagcta cttgcacttg gtcaggttca gcgcggagcg gatctcctcg gcgagctggt
     1321 aggcgtcgcc ctccttgacc atggcatccc agaacagctg cggctgctcg ttggtctcgt
     1381 cgttgcgcag gctgccctgg aaaaacttgt gctcgatctg catccgcttg accaccggga
     1441 cgacctcggc cgccgtcatc gagaagtcgg gacagacctg cgcgttcgcc ttgccgcccg
     1501 gcttggactc gttcgccatg ccggcgtcat cgtcccggcg gatcggcttg aactggatcg
     1561 tggtcgagat cccggcgtac ggcgacaccc tcacgccttc gagcaccacg cggtcggtgc
     1621 gcgcgaccac caccgtcacg acaccctccg cgccgacgtg ggcctcgccg tgcaggatgt
     1681 gggcgagccg atgtgcatcg agcggcgtgg tcggatgcgg cggcggcggc ggctgcggca
     1741 accggacatg ggtgacctcg agcgccgccc gggtggcgcg ggccagcgcg atcggatcgc
     1801 cgatgccgcg gaagtggatg aaccacacct ggggcagcat cggcaggtgc tggtggaacg
     1861 cctggaacac caggccgtgc tgcagtagcg cggcgatgaa cgggttggtc tccggctcga
     1921 gcaccgccat gtcggcgttg agcagggcct ggttcttgcc gagcggctgg aagtagagct
     1981 ccccgtggac ctcgaacgcc ggcgtgaagg tcaccccgag cggcccgtgc acctcgccga
     2041 tgtcggtccg catgatcgtg atgttcagga cgccgttggt gacctcaccc tgcgcgccca
     2101 cgatctgctc gacctgcttg atcggcatct gcgacgacga tcccgccgtg ctggtcggcg
     2161 cgttctcgtt cgggtccggc gccgccatgc tctccggcgt acaggccccg gccagcggag
     2221 cgagcagcag cggaatcaca atgtagtatt tcatattcct tccttctcct tgttcctctg
     2281 acgggcgcgt cgacgcaaag cactgtccgt gccgagcagc atcgcaatga aatcgcaatc
     2341 cagcgctcgc ccgccgcgcg ccatgcccac agtgatgccc aagcgcccgt gcagttctcg
     2401 tcgaggcccg ccgcgccatg cgggtgcgag attgccactg acgatccatg cccgggattg
     2461 ccagtctgca gtgccgcgcc gggcatttct tatgtgagat cgcaacgcga aatttcatcg
     2521 cgagagcgag gcggcgagac cggggccggc aagccccatg ggatcgctcg cgcgcagttg
     2581 tctaccctgg atcacagagc ccgatcgcgc acggtcgccg cgggcgcaag atcgccgggg
     2641 cttgccggtc gtcgccggcg cgcccgacgg tcagcgcagc tcgagcgcca ggctcaggat
     2701 ctcgtcgccg ggccgcaccg tcgcgcgggt ggcgccgcgc gtgtcggcct tggtcgccgc
     2761 gatcgcgagg tcgccggtcg gacagtcgtg gagccggaac tcgcc
//
LOCUS       DASVOR010001957         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488384,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001957 DASVOR010000000
VERSION     DASVOR010001957.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488384"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VF516_36355"
     CDS             <1..774
                     /locus_tag="VF516_36355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113277.1"
                     /translation="TAFQVAVFDGLTTTVRQLLPYAARDREVSYDPTSRLVATSDGAM
                     AMLLRYDPAAHVLGEPVRVGSAISPGKLVVVDPRQSGGIVALEIAETPDGLLVGELTE
                     ADLRPETTVQPRTTYRVPGQLRAVDRAGHLYTHRPFDHDDVVVYARDLPIARLPAVGE
                     LALRPSADGSRIAAFGSPRLVLLSGDGEVRWDSAQWSGADVGWTASGDLIVQFPTGVA
                     RVDLETGELADRRCGWGFGLSDHMLDVRHTGPSICDVLR"
     gene            complement(796..1773)
                     /locus_tag="VF516_36360"
     CDS             complement(796..1773)
                     /locus_tag="VF516_36360"
                     /inference="COORDINATES: protein motif:HMM:TIGR03001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8113278.1"
                     /translation="MTVTTPSLVRPFLDAAPAAMRPVLEGIADLDRRLWAIVAEGRAA
                     WPAFALDARDVVEFVARQTTAELADAALDGLRPADLYLACACARQVPGAIAAFDRDYM
                     REVDVALARMRIGPPRLHDVKQLVRQRLFVGGGTAAQPAGPGKIAEYGGRGDLRRWVR
                     SVAVRTCLNELRKGRREVLVDDEQLIAQHAIAPDDPEIEYMKRTYAHQFRAAFGEALA
                     QLGAREQTLLRYHHVDGLNIDEIGAIYRIHRVTAFRWIEKAKELLARSTLELLRGRLQ
                     LPANELDSVLRMIRSQIHLSLVRHLGGPSDSVADDAIELDPSELEEVDR"
BASE COUNT          232 a          653 c          673 g          253 t
ORIGIN      
        1 accgcgttcc aggtcgcggt gttcgatggc ctgaccacga cggtgcgcca gctgcttccg
       61 tacgccgcgc gcgaccgcga ggtgtcctac gatccgacga gccgcctggt ggcgaccagc
      121 gacggcgcga tggcgatgct gctgcgctac gatcccgccg cgcacgtgct cggcgagccg
      181 gtgcgggtcg gcagcgcgat ctcgccgggc aagctcgtcg tggtcgatcc gcggcagtcc
      241 ggcgggatcg tcgcgctgga gatcgccgag actcccgacg gcctgctggt cggcgagctc
      301 accgaggccg atctgcggcc ggagaccacg gtccagccgc gcacgaccta ccgcgtgccg
      361 ggccagctgc gcgccgtcga tcgcgccggc cacctgtaca cccaccggcc gttcgaccac
      421 gacgacgtgg tggtctacgc ccgcgatctc ccgatcgcgc ggctgcccgc ggtcggcgag
      481 ctcgcgctcc ggcccagcgc ggacggctcg cggatcgccg cgttcggcag cccgcggctc
      541 gtcctgctgt cgggcgacgg cgaggtccgc tgggacagcg cgcagtggag cggcgccgac
      601 gtcggctgga cggcgtccgg cgacctgatc gtgcagttcc cgaccggggt cgcccgggtc
      661 gacctcgaga ccggcgagct ggccgaccgg cgctgcggct ggggcttcgg gctgtccgac
      721 cacatgctcg acgtccggca caccggcccg tcgatctgcg acgtcctgcg ctgagccggc
      781 gcggcccggc gcggcctatc gatcgacctc ctcgagctcg ctcggatcga gctcgatcgc
      841 gtcgtcggcg acgctgtcgc tcggcccgcc gaggtggcgg accagcgaga ggtggatctg
      901 actgcggatc atccgcagca cgctgtcgag ctcgttggcc ggcagctgga gccggccgcg
      961 caggagctcc agggtcgagc gcgccaggag ctccttggcc ttctcgatcc agcggaacgc
     1021 ggtgacccgg tggatccggt agatcgcgcc gatctcgtcg atgttgaggc cgtcgacgtg
     1081 gtgatagcgg agcagggtct gctcgcgcgc cccgagctgg gcgagcgcct cgccgaacgc
     1141 ggcccggaac tggtgggcgt aggtccgctt catgtactcg atctcgggat cgtcgggggc
     1201 gatcgcgtgc tgcgcgatca gctgctcgtc gtcgaccagg acctcgcgcc ggcccttgcg
     1261 cagctcgttg aggcaggtcc tgaccgcgac cgagcgcacc cagcgccgga ggtcgccgcg
     1321 gccgccgtac tcggcgatct tgcccggtcc ggccggctgg gccgccgtgc cgccgccgac
     1381 gaacaggcgc tggcggacca gctgcttgac gtcgtgcagc cggggcggcc cgatccgcat
     1441 ccgggcgagc gcgacgtcca cctcacgcat gtagtcgcgg tcgaacgcgg cgatcgcgcc
     1501 ggggacctgg cgggcacagg cgcacgccag gtagagatcc gcagggcgca gcccgtcgag
     1561 cgccgcgtcg gcgagctcgg cggtggtctg gcgtgcgacg aactcgacca catcccgggc
     1621 gtcgagtgcg aacgcgggcc acgcggcgcg accctcggcg acgatcgccc acagccggcg
     1681 gtcgaggtcg gcgatgccct ccagcactgg ccgcatggcg gcgggcgcgg catcgaggaa
     1741 cgggcgcacc aggctcggcg tggtcaccgt cacgattagg ccattgccgg caacgaatgg
     1801 tcaaacgatc t
//
LOCUS       DASVOR010001958         3563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_225342,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001958 DASVOR010000000
VERSION     DASVOR010001958.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225342"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            117..1040
                     /locus_tag="VF516_36365"
     CDS             117..1040
                     /locus_tag="VF516_36365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113279.1"
                     /translation="MKRLLLVAIAACQPAGAGTPTTRPAPPPRVHLLNDLVGGWRWLL
                     RTPEAGTTRVESETWRLWPTPGVPTQLTGRYLREVEVRSDDRVPFACNQRPWYRQRAI
                     YDVTVQTAPSGFTVRETDYRTEPSPCDHGFRHLASYAGDADGERFLLHWDSGDQTLWQ
                     VDSDVAELPPAPWSARPEVTGPWRWDATSYDDAGNLRDESEWWEITRRNDRQLDLSYR
                     RRVTVRSPDGAAIACAGGPAWSYDDAYLLDGEREDEHWHFHERAYSPGEHPCLAAYPR
                     RSLDEATAEQRGDFLVLEWRGKRRQVLYRPE"
     gene            complement(1060..2700)
                     /gene="glgP"
                     /locus_tag="VF516_36370"
     CDS             complement(1060..2700)
                     /gene="glgP"
                     /locus_tag="VF516_36370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEX8113280.1"
                     /translation="MVPCPRVAYFCMEYGLDPAFAIYAGGLGILAGDHMKSAGDLHLP
                     VTGIGLLWDEGYTRQMVGVGGAIEDQYPKTARDVLRRIDTRVEVTVRGKKVPLRAWRV
                     DRYLTSNLYLLEPERDEDRWITQRLYGGGVEDRLAQEIVLGVGGVRLLTALGEKTDVF
                     HFNEGHAVFAGLELLRQQRFGGTTLSERIDRLRAHVVFTTHTPVPAGNEVHELDLMRK
                     MDADLGFTDAELEVIGGDPFSMTIAGLRLARIANGVAELHGQTAREMWKHVDGAAPII
                     SVTNGVHQPTWQDARIRAALASDKPRERQDVELWAAHQQMKGELLAEVARRTGVHLAL
                     DQLTIGFARRAAAYKRADLILGEEAALDRLFERGIQIVYSGKAHPRDLQGKALVGRLL
                     DAARRHPRHVVFLENYDMRIGALMTRGADVWLNNPRRPLEASGTSGMKAAMNGVPNVS
                     VLDGWWPEGCVHGVTGWKIGDPDPSDDPFDEQDTARVDRRDRELLHQVLEREVLVAYA
                     QRPRWLDIMRASIAMSQWRFSSDRQVEDYVARMYAQAG"
     gene            2945..3466
                     /locus_tag="VF516_36375"
     CDS             2945..3466
                     /locus_tag="VF516_36375"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEX8113281.1"
                     /translation="MKSRRPPVSPEDQALFLAAVDGARPLDPRDRLPVPPRPPSPVRV
                     VELPPEVKLSVEGDARRYAARAPGVSRAQLSELRAGKLRAEDTLDLHGQTVEIARQQL
                     REFLVAAHKLRRRCVLVVHGKGTHSEHGAPLREAVLAELLGPLSGLIHALTTAVPSQG
                     GEGATYIMLRGSR"
BASE COUNT          468 a         1384 c         1233 g          478 t
ORIGIN      
        1 ccccgcctat ccgcggcccg ccgccctctc cgacgcggcg atcgcccaga tcgtcgcaga
       61 cacgttcgcc cgccgccccg cctgagccgc ccggacgccc tgacgatgac ggtgacatga
      121 agcggctgct gctcgtcgcg atcgcggcct gccagccggc cggcgccggt acgccgacca
      181 cccgcccggc gccgccgccc cgcgtccatc tgctgaacga cctggtcggc ggctggcgct
      241 ggctgctccg caccccggag gccgggacca cccgcgtcga gtccgagacc tggcggctgt
      301 ggcccacgcc cggcgtgccg acccagctca ccggccgcta cctccgcgag gtcgaggtcc
      361 ggtccgacga ccgtgtcccg ttcgcctgca accagcgccc gtggtaccgc cagcgcgcga
      421 tctacgacgt cacggtccag accgcgccca gcgggttcac cgtgcgcgag accgactacc
      481 gcaccgagcc cagcccgtgc gaccacgggt ttcgccacct cgccagctac gccggggacg
      541 ccgacggcga gcgcttcctg ctccactggg acagcggcga ccagaccctg tggcaggtcg
      601 acagcgacgt cgccgagctc ccgcccgccc cgtggtcggc ccgccccgag gtcaccgggc
      661 cgtggcggtg ggacgccacc tcgtacgacg acgccggcaa cctgcgcgac gagagcgagt
      721 ggtgggagat cacgcggcgc aacgaccgcc agctcgacct cagctaccgc cgccgggtca
      781 ccgtccggtc accggacggc gcggccatcg cgtgcgccgg cggcccggcg tggagctacg
      841 acgacgccta cctgctcgac ggcgagcgcg aggacgagca ctggcacttc cacgagcgcg
      901 cctactcgcc cggcgagcac ccctgcctgg cggcctaccc gcggcgcagc ctcgacgagg
      961 ccaccgccga gcagcgcggc gacttcctcg tgctggagtg gcgcggcaag cgccgccagg
     1021 tgctgtaccg cccggagtag cgtgaccggc gccgcgccgc tatccggcct gggcgtacat
     1081 cctggcgacg tagtcctcga cctggcgatc cgacgagaac cgccactgcg acatcgcgat
     1141 gctggcgcgc atgatgtcca gccagcgcgg ccgctgagcg taggcgacga gcacctcgcg
     1201 ctccagcacc tggtgcagca gctcgcgatc gcgccggtcg acccgcgcgg tgtcctgctc
     1261 gtcgaacggg tcgtcgctgg ggtcgggatc gccgatcttc cagccggtga cgccgtggac
     1321 gcagccctcc ggccaccagc cgtcgagcac cgagacgttg ggcacgccgt tcatcgccgc
     1381 cttcatcccc gaggtccccg atgcctccag cggccgccgc gggttgttga gccagacgtc
     1441 ggcgccccgc gtcatcagcg cgccgatccg catgtcgtag ttctcgagga acaccacgtg
     1501 gcgcgggtgg cgccgggcgg cgtcgagcag ccggccgacc agcgccttgc cctgcaggtc
     1561 gcgcgggtgc gccttgccgg aatagacgat ctggatcccg cgctcgaaca gccggtccag
     1621 cgcggcctcc tcgccgagga tcaggtcggc gcgcttgtag gccgccgcgc gccgcgcgaa
     1681 cccgatcgtc agctggtcga gcgcgaggtg gaccccggtg cgccgggcga cctcggcgag
     1741 cagctcgccc ttcatctgct ggtgcgcggc ccacagctcg acatcctggc gctcgcgcgg
     1801 cttgtcggac gccagcgcgg cgcggatccg cgcgtcctgc caggtcggct ggtggacgcc
     1861 gttggtcacg ctgatgatcg gcgcggcgcc gtcgacgtgc ttccacatct cgcgcgcggt
     1921 ctggccgtgc agctcggcga cgccgttggc gatccgcgcc agccgcagcc cggcgatcgt
     1981 catcgagaac ggatcgccgc cgatcacctc gagctcggcg tcggtgaacc cgagatcggc
     2041 gtccatcttg cgcatcaggt cgagctcgtg gacctcgttg cccgccggca ccggcgtgtg
     2101 cgtggtgaac acgacatgcg cgcgcagccg gtcgatccgc tcgctcagcg tggtcccgcc
     2161 gaaccgctgc tggcgcagca gctccagccc ggcgaacacc gcatggccct cgttgaagtg
     2221 gaacacgtcg gtcttctcgc ccagcgcggt gagcaggcgc acgccgccga ccccgagcac
     2281 gatctcctgc gccagccggt cctcgacccc gcccccgtac agccgctggg tgatccagcg
     2341 atcctcgtcg cgctcgggct ccagcaggta gaggttcgag gtcaggtagc ggtcgacccg
     2401 ccaggcacga agcggcactt tcttgccgcg caccgtgacc tcgacccggg tgtcgatccg
     2461 gcgcagcacg tcgcgcgcgg tcttgggata ctggtcctcg atcgcgccgc cgacgccgac
     2521 catctgccgc gtgtagccct cgtcccacaa cagcccgatg ccggtgactg gcaaatgcag
     2581 gtcaccggcg gacttcatgt gatcaccggc caggatcccg aggccgccgg cgtagatcgc
     2641 gaacgcgggg tcgagcccgt actccatgca gaagtacgcg acgcggggac acggaaccat
     2701 ggacgtactg cctccagggc gcacggtgcc gaacccggcg cgccgctgca agcggcgctg
     2761 cggaaaagcc gcgctgttgc cagatacaac gcggggttag cggatgttgc gcgagcccgg
     2821 gggggacccc gaccgctccg ccggtgggcg gggccaggcc cccgtgggag atcgacgtcg
     2881 ctctgtcagc cggtgccgtg cgatttcttg gcagcgcggc ccgccgtcga gtacagaatg
     2941 agggatgaag tcgcgccggc cccccgtatc cccggaggac caggcgctgt tcctggccgc
     3001 ggtcgacggc gcgcgcccgc tggacccccg cgaccggctg ccggtgccgc cgcgcccgcc
     3061 gtccccggtg cgggtcgtcg agctgccgcc cgaggtcaag ctctccgtcg agggcgatgc
     3121 ccggcgctac gccgcccgcg cgcccggggt cagccgcgcc cagctgtccg agctgcgcgc
     3181 cggcaagctg cgcgccgagg acaccctcga cctgcacggc cagaccgtcg agatcgcccg
     3241 gcagcagctg cgagagttcc tggtcgccgc ccacaagctc cgccggcgct gcgtgctggt
     3301 ggtccacggc aagggcaccc actccgagca cggcgcgccg ctgcgcgagg cagtgctcgc
     3361 cgagctgctc ggcccgctgt ccggtctcat ccatgcgctg accaccgccg tgccgtcgca
     3421 gggcggcgag ggcgcgacct acatcatgct gcgtggctcg cggtgagggc gctgctggtc
     3481 gcctcgctcg cgctcggggt tctggccccg gccggccctg tcaccgcccg cccggcggtg
     3541 gtgcgcggcc gcagcgtcgc cag
//
LOCUS       DASVOR010001959         4259 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341625,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001959 DASVOR010000000
VERSION     DASVOR010001959.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4259
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341625"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1929)
                     /locus_tag="VF516_36380"
     CDS             complement(64..1929)
                     /locus_tag="VF516_36380"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8113282.1"
                     /translation="MKVHLIGIGGTGMGAVAGLLAEAGHQVRGSDAAVYPPMSDQLAE
                     LGVPVMMPYAEDNLAWQPELVVVGNVHGKDHVEVLAAQARGIPLTSFPAVIGDQLLDG
                     KHAIVVCGTHGKTTTTSLVAHVLTEAGHDPSLFVGGVPVTLGRGYRTGRGAEFVLEGD
                     EYDTAFFDKGPKFLHYRPETVILTSVELDHVDIFPSFDAVRDVFRKLVAAIPPSGLLV
                     VCADSAEAIAVARHASCRVEQYAVIDDSNEPPPEVTWWAQYAEVGKSGRVAFDVFHRI
                     AGPGDGRGERVEHFESLLVGRHNVANCVAAIAVARACGVPPGDLVRGVASFAGVRRRQ
                     ELRGIAGGVTVLDDYAHHPTAVRETLKALRKRYPKRRLIAVYEPRSATSRRKTFQAEF
                     AEAFAHADEVIVGRLFDPSRIPADERFDPERLALDLHRSGTKASYVPEVDTIVKQLAE
                     SAGPGDVVCVLSSGSFDGLHDKLLDEIGDSMRPAHREDMAAVRQMLAAAGLEEPARDD
                     QQGSFFVLRNEDGVVGCVALEVLGADAILRALAVDPRFRGAGYGWMLADTAVNQARWR
                     GVRRIYLLTETASDFFAAKFGFRVVDRSTLSRQVAASEVFSRPAAAHQIAMRLDL"
     gene            complement(2056..2826)
                     /locus_tag="VF516_36385"
     CDS             complement(2056..2826)
                     /locus_tag="VF516_36385"
                     /inference="COORDINATES: protein motif:HMM:NF045440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1338 domain-containing protein"
                     /protein_id="HEX8113283.1"
                     /translation="MTTVELLELLWRDYVAAAPQAEEIHAILTGRGEILCNDHVALRT
                     FAVPGIGGDALARPFAALGWRPGGCYEFLGGLRARAWQHDDPALPRILISELAIDHLS
                     PRAQAVIGTLVDQLPDRFCERGDLAWAGRPWRVAYADYQALRAESAYAAWVAAFGFLV
                     NHFTIDAGALSTFPDLAALGAFLVEHGFALDDPGTIRGSRGERIERAATRPDPVAVEF
                     ADTIARIPSCHYELVRRFPMPSGELFTGFVAASAGAAA"
     gene            complement(2870..3409)
                     /locus_tag="VF516_36390"
     CDS             complement(2870..3409)
                     /locus_tag="VF516_36390"
                     /inference="COORDINATES: protein motif:HMM:NF018508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1232 domain-containing protein"
                     /protein_id="HEX8113284.1"
                     /translation="MAAHIDVFRGWADTIRQDIDAFKALLDSPRADAASRQLAAAALS
                     YLVSRMDLVPDWNEGIGVLDDVMVLRVCAQLSQGCDRGTLSTAAEVSLERMANEADKI
                     TAFLGGALYDKLRAYCAKLVDRPVRGRTSAQLAADAAARQALYGEVEDELKRTVPIVI
                     NDPADAEVRLKAYLTHKLR"
     gene            3593..3904
                     /locus_tag="VF516_36395"
     CDS             3593..3904
                     /locus_tag="VF516_36395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113285.1"
                     /translation="MRMLIATLLVLAGACTGAAPGGSRTCAGNLYDACLTEHDCMMGN
                     CVNFMAARFQVCSVSCTVGDDTPCMTNDRGQKASCMPISAGSSNGVCTPPAENDCTRA
                     P"
     gene            complement(3935..>4259)
                     /locus_tag="VF516_36400"
     CDS             complement(3935..>4259)
                     /locus_tag="VF516_36400"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8113286.1"
                     /translation="HAAVEHTPARTSRPRPEPHADGRAEARAEARIEPSVGQGTLMIS
                     SKPPCEIAIDGRTTGLTTPQRSISLSAGNHRITLLNSEKSIHKVVNVQIIANTTAKII
                     EDLMQ"
BASE COUNT          612 a         1550 c         1498 g          599 t
ORIGIN      
        1 cgcgcgccgc ggcgaggagc tccgaggggc cgcgcgcgct caccgccggg gtcatggccc
       61 cgctcataga tcgaggcgca tcgcgatctg gtgcgccgcg gccggccggc tgaacacctc
      121 gctcgccgcg acctgccggg acagagtcga gcgatcgacg acccggaatc cgaacttggc
      181 ggcgaagaaa tcgctcgccg tctcggtcag caggtagatc cgccgcaccc cgcgccagcg
      241 cgcctggttg accgcggtgt cggcgagcat ccagccgtag cccgcgccgc ggaaccgcgg
      301 atcgaccgcg agcgcgcgca ggatcgcgtc ggcgccgagc acctcgagcg cgacgcagcc
      361 gaccacgccg tcctcgttgc gcagcacgaa gaagctgccc tgctgatcgt cgcgcgccgg
      421 ctcctcgagc ccggccgccg ctagcatctg gcggaccgcc gccatgtcct cgcggtgggc
      481 cggacgcatc gagtcgccga tctcgtcgag cagcttgtcg tgcaggccgt cgaagctgcc
      541 cgacgacagc acgcagacca cgtcgccggg ccccgcgctc tcggcgagct gcttgacgat
      601 ggtgtcgacc tcggggacgt acgacgcctt ggtgccgctg cggtggaggt cgagcgcgag
      661 ccgctcggga tcgaaccgct cgtcggccgg gatcctcgac ggatcgaaca gccgcccgac
      721 gatcacctcg tcggcgtggg cgaacgcctc cgcgaactcg gcctggaacg tcttgcgccg
      781 gctggtcgcg gagcgcggct cgtagaccgc gatcagccgg cgcttggggt agcgcttgcg
      841 cagcgccttg agcgtctcgc gcaccgcggt cggatggtgc gcgtagtcgt cgagcacggt
      901 gaccccgccg gcgatgccgc gcagctcctg ccgccgccgt acgccggcga agctcgcgac
      961 gccgcgcacg agatcgcccg gcggcacgcc gcacgcgcgg gcgaccgcga tcgccgcgac
     1021 gcagttcgcc acgttgtggc ggccgaccag cagggactcg aagtgctcga cgcgctcgcc
     1081 gcgcccgtcg cccggcccgg cgatgcggtg gaacacgtcg aacgcgaccc gcccgctctt
     1141 gccgacctcg gcgtactggg cccaccacgt gacctcgggc ggcggctcgt tgctgtcgtc
     1201 gatcaccgcg tactgctcga cccggcagct cgcgtggcgg gcgaccgcga tcgcctcggc
     1261 ggagtcggcg cacaccacga gcagcccgct cggcgggatc gccgcgacca gcttgcggaa
     1321 cacgtcgcgc accgcgtcga acgacgggaa gatgtcgacg tggtcgagct cgaccgaggt
     1381 caggatcacg gtctcgggcc ggtagtgcag gaacttgggt cccttgtcga agaacgcggt
     1441 gtcgtactcg tcgccctcga gcacgaactc ggcgccgcgc ccggtccggt agccgcggcc
     1501 gagcgtgacc ggcacgccgc cgacgaacag cgacggatcg tgcccggcct cggtcaggac
     1561 gtgcgcgacc agcgacgtcg tcgtggtctt gccgtgggtg ccgcacacca cgatcgcgtg
     1621 cttgccgtcg agcagctggt cgccgatcac cgccgggaac gaggtcagcg ggatgccgcg
     1681 cgcctgcgcc gcgagcacct cgacgtgatc cttgccgtgg acgttgccga ccacgaccag
     1741 ctcgggctgc cacgccaggt tgtcctcggc gtacggcatc atcaccggga cgccgagctc
     1801 ggccagctga tcgctcatcg gcgggtacac cgcggcatcg gagccacgga cctggtggcc
     1861 cgcttccgcg agcagccccg ccaccgcccc catgccggtg cccccgatgc cgatcagatg
     1921 gaccttcacg acggcgccaa gctacccagc cgggtccggc gggtaaagcg tttcggcacg
     1981 tggtttgccg gttcaccggc gcatttcctg gccgcggcga cgcgatcggt gagccggggc
     2041 ttccgcgccg cggcgctacg ccgcagcccc cgcgctcgcg gcgacgaagc cggtgaacag
     2101 ctctccgctc ggcatcggaa accgccgtac gagctcgtag tggcagctcg ggatccgcgc
     2161 gatggtgtcg gcgaactcga ccgcgaccgg atccgggcgc gtcgcggcgc gttcgatccg
     2221 ctcgccgcgc gagccgcgga tcgtgccggg gtcgtcgagc gcgaagccat gctcgacgag
     2281 gaacgcgccg agggccgcga gatcggggaa cgtcgacagc gcgccggcgt cgatcgtgaa
     2341 gtggttgacc aggaagccga acgccgcgac ccacgccgcg tacgccgact ccgcgcgcag
     2401 cgcctggtaa tcggcgtacg ccacgcgcca cggccggccg gcccacgcca gatcgccgcg
     2461 ctcgcagaac cggtcgggca gctggtcgac cagcgtgccg atcaccgcct gcgcccgcgg
     2521 cgacaggtgg tcgatcgcga gctcgctgat caggatccgg ggcagcgcgg gatcgtcgtg
     2581 ctgccaggcg cgcgcccgca ggcccccgag aaactcgtag cacccgcccg gccgccagcc
     2641 gagcgcggcg aacggccggg cgagcgcgtc gccgccgatg cccggcaccg cgaaggtgcg
     2701 aagcgccacg tgatcattgc acaagatctc gccgcgcccg gtcaggattg cgtggatctc
     2761 ctcggcctgc ggcgcagcgg cgacgtagtc gcgccacagc agctccagca gctcgacggt
     2821 cgtcaccgag cttgggtatg acaagccccg gcgcgcttgc aaagctctgc taacggagct
     2881 tgtgcgtcag gtaggccttg agccggacct cggcgtcggc cggatcgttg atcacgatcg
     2941 gaaccgtgcg cttgagctca tcctcgacct cgccgtacag cgcctggcgg gccgcggcat
     3001 ccgccgccag ctgagccgag gtccggccgc gcaccgggcg gtcgaccagc ttggcgcagt
     3061 acgctcgtaa cttgtcgtag agcgcgccgc cgaggaacgc cgtgatcttg tcggcctcgt
     3121 tcgccatccg ctccaggctg acctcggccg cggtcgacag cgtcccgcga tcgcagccct
     3181 ggctcagctg cgcgcacacc cggagcacca tcacgtcgtc gagcaccccg atgccctcgt
     3241 tccagtccgg gacgagatcc atccggctca ccaggtacga cagcgccgcg gcagcgagct
     3301 gcctggacgc cgcgtcggcg cgcggcgagt cgagcagcgc cttgaacgcg tcgatgtcct
     3361 ggcggatcgt gtcggcccag cccctgaaga cgtcgatatg agcagccacg tgccctcctt
     3421 accgtcgacg gtactgccaa gccggcgaag gtttcaaccg caaggcggag ctgtccgagg
     3481 gttgttccga gggccgaggg gtccgggttc gatgtgagcg atgcacctgc ggtggccggg
     3541 gcgcggcccg cgtcaccggg cggcgatcgc aaccctgata agctctcgcg ccatgcggat
     3601 gttgatcgcg acgctgctcg tgctggccgg cgcctgcacc ggcgcagccc ccggcggatc
     3661 ccggacgtgc gccggcaacc tctacgacgc ctgcctcacc gagcacgact gcatgatggg
     3721 caactgcgtc aacttcatgg ccgccaggtt ccaggtctgc tcggtgagct gcacggtggg
     3781 cgacgatacg ccctgcatga ccaacgaccg cggtcagaag gcgagctgca tgccgatctc
     3841 ggcgggcagc tcgaatgggg tctgcacgcc gcccgccgag aacgactgca cgcgcgcacc
     3901 gtagccacgc cacgcggacc gtcgcagcgc ccggtcactg catcaggtct tcgatgatct
     3961 tcgcggtggt gttcgcgatg atctgaacgt tgacgacctt gtggatcgac ttctcgctgt
     4021 tgagcagcgt gatccggtgg ttgccggcgg acagcgagat cgagcgctgc ggcgtggtca
     4081 gcccggtcgt ccggccgtcg atggcgatct cgcacggcgg cttgctggag atcatcagcg
     4141 tgccctgccc gaccgatggc tcgatgcgag cctcggcacg agcctcggcg cgaccgtcgg
     4201 cgtgcggctc gggtcgcggc cggctggtgc gggccggcgt gtgctcgacc gccgcgtgc
//
LOCUS       DASVOR010001960         1840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_21820,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001960 DASVOR010000000
VERSION     DASVOR010001960.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21820"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF516_36405"
     CDS             complement(<1..822)
                     /locus_tag="VF516_36405"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113287.1"
                     /translation="MAQMASHRELAEAWLAEDPDPQTAAELRALLSRAGAGDAAAQKD
                     LAERFTGALEFGTAGLRGLLGAGPQRMNRALVRKVSAGLAAYLLAAVPDARRRGVVIG
                     HDARHLSRELSEDTARILGGAGIRSYLAHRPWPTPMTAWAVTAQQACAGVMVTASHNP
                     PAYNGYKVYWGNGAQIVPPHDTGIAAEVARVGSIRDIAMPSLDELRAAGTVVDLTEAL
                     HDDYLAQVVALRASPGVDGRDLVIAYTPLHGVGARSVEPALRLAGFPGVHTEPSQR"
     gene            complement(849..1529)
                     /locus_tag="VF516_36410"
     CDS             complement(849..1529)
                     /locus_tag="VF516_36410"
                     /inference="COORDINATES: protein motif:HMM:NF017319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8113288.1"
                     /translation="MLARSLAPLLALGLALGPSVAAAQPKAQPPAPAGQATQAAPATK
                     TGPGTQAVRAANDTIAGLLKQKAAPGSKEEKDAAAKVTTSVRSFLDIDQLGKRAMVDN
                     WGKLSKAQQDQFLALLRELIEDNYVRGLRANLDYQVDYTGESTDADGNVVVTTKINTK
                     RRGRPYAIEVDYVLVKDGDKLRAWDVKTDGVGLVENYRTMFDKIIDKDGFDGLIAKMK
                     KKQAASGS"
     gene            complement(1572..>1840)
                     /locus_tag="VF516_36415"
     CDS             complement(1572..>1840)
                     /locus_tag="VF516_36415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113289.1"
                     /translation="ADTALADATAAHDKVAAAGQGEQAARTWLASVLQAEAIGAAESR
                     DLADAYIAWFQMRARWAQAVFQWNVAVVRLDRAAGEFHAAAHRP"
BASE COUNT          216 a          632 c          707 g          285 t
ORIGIN      
        1 gcgctgcgac ggctcggtgt ggacgccggg gaacccggcg agccgcagcg cgggctcgac
       61 cgagcgcgcg ccgacgccgt gcagcggcgt gtaggcgatc accaggtcgc ggccgtcgac
      121 gccggggctg gcgcgcagcg ccacgacctg ggcgaggtaa tcgtcgtgca gcgcctcggt
      181 caggtcgacc acggtgccgg cggcgcgcag ctcatccagc gacggcatcg cgatgtcccg
      241 gatcgagccg acccgggcga cctcggcggc gatcccggtg tcgtgcggcg ggacgatctg
      301 ggcgccgttg ccccagtaga ccttgtagcc gttgtaggcc ggggggttgt gcgacgcggt
      361 gaccatgacg ccggcgcacg cctgctgcgc ggtcaccgcc cacgcggtca tcggcgtcgg
      421 ccacggccgg tgcgcgaggt agctccggat gccggcgccg ccgaggatcc gggcggtgtc
      481 ctccgacagc tcgcgcgaca ggtggcgcgc gtcgtggccg atcaccacgc cgcgccggcg
      541 ggcgtcgggc acggcggcga gcaggtaggc ggcgagcccg gcgctgacct tgcggaccag
      601 cgcgcggttc atccgctgcg gcccggcgcc gagcagtccg cgcagcccgg cggtgccgaa
      661 ctcgagggcg ccggtgaacc gctcggcgag atccttctgc gcggcggcgt cgccggcgcc
      721 tgcgcgtgac agcagcgcac gtagctcggc ggcggtctgg gggtcgggat cctcggcgag
      781 ccacgcctcg gcgagctcgc ggtgcgaagc catctgcgcc atggctcgca gcctacaacg
      841 cgcgaccgtc aggagccgct cgcggcctgc ttcttcttca tcttggcgat caggccgtcg
      901 aacccgtcct tgtcgatgat cttgtcgaac atcgtgcggt agttctcgac cagcccgacg
      961 ccgtcggtct tgacgtccca ggcgcgcagc ttgtcgccgt ccttgaccag cacgtagtcg
     1021 acctcgatcg cgtacggccg gccccggcgc ttggtgttga tcttggtcgt gaccacgacg
     1081 ttgccgtcgg cgtcggtcga ctcgccggtg tagtcgacct ggtagtcgag gttggcgcgc
     1141 aggccgcgca cgtagttgtc ctcgatcagc tcgcggagca gggcgaggaa ctgatcctgc
     1201 tgcgccttgg atagcttgcc ccagttgtcg accatcgcgc gcttgccgag ctgatcgatg
     1261 tcgaggaagc tgcgcacgct cgtggtgacc ttggcggcgg cgtccttctc ctccttggac
     1321 ccgggcgcgg ccttctgctt gagcaggccg gcgatcgtgt cgttggcggc ccgcaccgcc
     1381 tgggtgccgg ggccggtctt cgtcgccggg gcggcctggg tggcctgacc ggcaggcgcg
     1441 gggggctggg ccttgggctg cgccgcggcc accgagggcc ccagggcgag accgagggcg
     1501 aggagcggtg cgagggatcg ggcaagcatg gggactcctg agactcctga gctgtgcagc
     1561 tctggttcgg gttagggacg gtgggcggcg gcgtggaatt cacccgccgc gcgatcgagg
     1621 cgtaccacgg cgacgttcca ttggaagacc gcttgcgccc agcgcgcgcg catctggaac
     1681 caggcgatgt aggcgtccgc caggtcgcgg gactcggcgg ctccgatcgc ctcggcctgc
     1741 agcaccgacg ccagccaggt ccgcgcggcc tgctcgccct ggccggcggc cgcgaccttg
     1801 tcgtgggcgg cggtcgcgtc ggccagcgcg gtgtcggcgt
//
LOCUS       DASVOR010001961         4872 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_21826,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001961 DASVOR010000000
VERSION     DASVOR010001961.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4872
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21826"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            546..1043
                     /locus_tag="VF516_36420"
     CDS             546..1043
                     /locus_tag="VF516_36420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113290.1"
                     /translation="MNDSDATDRTTRQGQNQRRHAPDGKPHVSIGSFRRDPMYPQVEP
                     AVAAILASSKVVAPVDVIVRLGWLSTADLENWRFGRVPYPERVIRCNLTRLSRFLRIL
                     GFHCHDLNLTASQTAYVKWGKGRRAALRFTKTGDPPLERAYARHFVWPGKGPFHPPRE
                     RDDLA"
     gene            complement(1256..2593)
                     /locus_tag="VF516_36425"
     CDS             complement(1256..2593)
                     /locus_tag="VF516_36425"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8113291.1"
                     /translation="MGKTDEGNATTVAGFAWRARLSHVTPISLPVGRFEDDGPIGDGG
                     RSTVHRIFDKTLLRYSAMKRLAPELKPYPAERQRFIEEAQIAAQLDHPNVVPVHELSL
                     TDDGDLYFTMKLVEGRNLADLLDRLDNHPRSFDLVAPFLDILVKVCDAIAFAHSRGII
                     HRDIKPGNIMVGNFGEVYVMDWGVARLVDGKSDVTTARGAHAQSLDPHGHPIGTPAYM
                     APEQAAGLHDETDERSDVFALGATLYHILTGQAPYDNVAIDLAGMIAAARRCEIRPAE
                     KLSGAARLPRGLSRIAMKAMARDPAHRYPSAAAFKDDLLVQLRGSADLPRCIFSAGDT
                     IVAEGEPGDAAYIIVSGTCRVFKTVNHEDVDLRILGPGEVFGETAIFSRAPRSASVQA
                     LETLVLQVVSPEVLEEAVGLHGGLGLFIRTLAERFREVDERLSQLDAELRVRR"
     gene            complement(2633..3754)
                     /locus_tag="VF516_36430"
     CDS             complement(2633..3754)
                     /locus_tag="VF516_36430"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEX8113292.1"
                     /translation="MSPPTRTRRGASVLLIDDQPIIGAAVARMLADEPATRFHHCCEP
                     GSALADAERVQPTVILLDMVMPDVDGLTMLRYLRAAPVTRDVPIVVMSSRTEPTLKAE
                     AFTLGADDYVVKLPDPLELVARVRYHSRAYNNALDAGDAWDALEESRRQLEARNQLIR
                     KTFGRYLSDDIVASLLETQAGLHLGGEARRVTILMADLRGFTPMCEQLTPAEVVGLLN
                     NYLGAMGDLVTDHGGTVDEFIGDAILAVFGAPIHHPDHARRAIACAVAMQRAMPEVNA
                     RNRAAGLPSVEMGIGLGTGEVVVGNIGSERRAKYGVVGGRVNLTSRIAGLAGGGQILA
                     SEPTILEAPGAARYSAPREVALKGVTGLVAVHEVSAVSR"
     gene            complement(3751..>4872)
                     /locus_tag="VF516_36435"
     CDS             complement(3751..>4872)
                     /locus_tag="VF516_36435"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113293.1"
                     /translation="DGRGVDFAAVRHRVAEQGLAPADRVFHMSEAELTDFLFQPGFTT
                     AAEVGDVSGRGVGLDVVRDVVHQLGGKLRAEPQAGRGMTFQLELPVTRSVLRTLLVEI
                     AGEPFAFPLVRVERVLQIAAARVSSLEGRQYLDLDGQNIGLIAAHQVLELAPGEARDE
                     LCVVVISDQLDTYGLAVDRFLGERDLVVQPIDPRLGKIADVAATSIMQDGSPVVILNV
                     DDLVHSVNALLAGSRLQPVRRLREPTRKKRVLVIDDSTTVREALRRALDQRGYAVDLA
                     ADGVDGWAAVRTGDYALVVTDVDLPRLSGHDLIARMRAEPRLAHTPIVVVSYKDREED
                     RRAGLAAGADRYLDKTSFHDDQLLTAVAELIGGPGATVR"
BASE COUNT          747 a         1542 c         1852 g          731 t
ORIGIN      
        1 cagacgcgcg gcagcgggga ccgccgtcat tccaacctcg acgatggcag tgctgttgcg
       61 agccctgagc ggtgcgatgg ggcgcgcctc gaccgccgtt accaggccgc gctcgatgga
      121 ctcgcggcgc tgcacatcca cgtctgccaa ccaccctcga ccgtcgctgc gctcagcctc
      181 cacccgccgg agttcgagga ccaccggcga tcggcgaagg agttcggaaa gacgcgttag
      241 gccgaaaaat gacgccggaa actgactcaa cttgctgaat gcgctcggga aatcatcact
      301 gcggaaaagt gctgagcgcg taagaagttg aaatcagtga ctcttgacgg gatttaggtt
      361 cctgtccgta acagggtccg ggttcgagtc ccggcgctcg cacgcgactt ttcgaggact
      421 tggaaagtcc ccaaaggctt ctcgaaagtc acgaaaggct ttcgcgaagc tccgtatcgc
      481 cggcggccac gcggtgcggc gaggttggcc cgcgcgcggt cgacaaacag tggcagagtt
      541 gccgcgtgaa cgactccgac gctaccgatc ggaccaccag acaaggacag aatcaacgca
      601 ggcatgcgcc cgacggcaag ccgcatgtgt cgatcggttc gttccgccgc gatccgatgt
      661 atccccaggt agaacccgcg gtggctgcga ttctcgcaag tagcaaggtc gtcgcgccag
      721 tcgacgtgat cgttcggctg ggctggctgt ccaccgccga tctcgagaac tggcggttcg
      781 ggcgcgtacc gtaccccgag cgcgtaatcc gctgcaacct cacccggctc agccgcttcc
      841 ttcgcatcct gggctttcat tgtcatgacc tgaacctcac cgcgtcgcag actgcgtacg
      901 tgaagtgggg caagggccgg cgggcagcgc tgcgcttcac caagaccggt gatcctccgc
      961 tcgagcgagc ctacgcgcgc catttcgtct ggccgggcaa gggcccgttt catccaccac
     1021 gagagagaga cgatctcgcg tgagatgcgg atagcgactc tggctcgtgc acgatgcccg
     1081 agctgacgtc gagtgtcgcg acctcggcgt ccagctcggc gactgggtcc gggcatgttc
     1141 gatgccggcg atcctcggca gtcgaggccc acgcagccag gagctcgcag cagcaggcat
     1201 tggagagacc tcacacgata acacgttatc acattcgcca gaacaacgac gggcactatc
     1261 gccggacccg cagctcggcg tcgagctggc tgaggcgctc gtcgacctcg cggaaccgct
     1321 cggcgagcgt gcggatgaac aggccgaggc cgccgtggag gccgacggcc tcttcgagca
     1381 cctcggggga gaccacctgg aggacgaggg tctcgagcgc ctgcacgctg gcgctgcgcg
     1441 gcgcgcgcga gaagatggcg gtctcgccga agacctcgcc cgggccgagg atgcggagat
     1501 cgacgtcctc gtggttgacg gtcttgaaca cgcgacaggt gccggagacg atgatgtacg
     1561 cggcatcgcc cggctcgccc tcggccacga tcgtgtcgcc ggcgctgaag atgcagcgcg
     1621 gcaggtcggc gctgccgcgg agctggacga ggaggtcgtc cttgaaggcg gctgcgctgg
     1681 gatagcggtg cgcgggatcg cgcgccatcg ccttcatcgc gatgcgggac agcccgcgcg
     1741 gcaggcgcgc ggcgccggac agcttctcgg cggggcggat ctcgcagcgg cgggcggcgg
     1801 cgatcatgcc ggcgaggtcg atggcaacgt tgtcatacgg ggcctggccg gtgaggatgt
     1861 ggtacagggt cgcgccgagc gcgaacacat cggagcgctc gtcggtctcg tcgtggaggc
     1921 cggcggcctg ttcgggggcc atgtacgccg gcgtgccgat cgggtgcccg tgcgggtcga
     1981 gggactgcgc gtgcgcgccg cgggcggtcg tgacgtcgct cttgccgtcg acgaggcggg
     2041 cgacgcccca gtccatgacg tagacctcgc cgaagttgcc gaccatgatg ttgccgggct
     2101 tgatgtcgcg gtggatgatg ccgcggctgt gagcgaacgc gatggcgtcg cagaccttga
     2161 cgaggatgtc gaggaacggc gcgacgaggt cgaagctgcg cgggtggttg tcgagccggt
     2221 ccagcagatc ggcgaggttc cggccctcga cgagcttcat ggtgaagtag aggtcgccgt
     2281 cgtcggtgag gctgagctcg tggaccggga cgacgttggg gtggtcgagc tgggccgcga
     2341 tctgggcctc ctcgatgaag cgctggcgct cggcgggata gggcttgagc tcgggcgcca
     2401 ggcgcttcat ggcgctgtag cggagcagcg tcttgtcgaa gatccggtgg accgtgctgc
     2461 ggccgccgtc gccgatcgga ccgtcgtcct cgaaccggcc caccgggagc gagatcggag
     2521 tgacgtgaga cagccgggct cgccacgcga agccggcgac ggtggtcgcg ttgccctcgt
     2581 cggtcttgcc catgcccgga gtgtgcgatg cgcggtgcgc gactgtccac gttcaccgac
     2641 tgacagcgga gacttcgtgg accgcgacga ggccggtgac gcccttgagg gcgacttcgc
     2701 ggggggcgct gtaccgggca gcgccgggcg cctccaggat ggtgggctcg gaggcaagga
     2761 tctgaccgcc gccagcgagg ccggcgatgc gcgaggtcag gttgacgcgg ccgccgacga
     2821 cgccgtactt ggcgcggcgc tcggagccga tgttgccgac gacgacctcg ccggtgccca
     2881 ggccgatgcc catctcgacg ctcgggaggc cggcggcgcg gttgcgggcg ttgacctcgg
     2941 gcatggcgcg ctgcatggca acggcgcacg cgatcgcgcg ccgggcatgg tcggggtggt
     3001 ggatcggagc gccgaacacg gcgaggatgg cgtcgccgat gaactcgtcg acggtgccgc
     3061 cgtggtcggt gacgagatcg cccatggcgc cgagatagtt gttgagcagg cccacgacct
     3121 cggcgggggt gagctgctcg cacatcgggg tgaagccgcg caggtcggcc atgaggatcg
     3181 tgacccggcg cgcctcgccg ccgaggtgaa gaccggcctg ggtctcgagc aggctggcga
     3241 cgatgtcgtc cgagaggtag cggccgaagg tcttgcggat gagctggttt ctggcctcga
     3301 gctggcgccg gctctcctcg agggcgtccc acgcatcgcc cgcgtcgagc gcgttgttgt
     3361 aggcgcgcga gtggtaacgc acgcgcgcca cgagctcgag cgggtccggc agcttgacga
     3421 cgtagtcgtc ggcgccgagc gtgaacgcct cggccttgag ggtgggctcg gtccggctgg
     3481 acatcacgac gatggggacg tcgcgggtga ccggcgcggc gcggagatag cgcagcatgg
     3541 tgagcccgtc gacgtcgggc atgaccatgt cgaggaggat gacggtgggc tggacgcgct
     3601 cggcgtcggc cagggcgctg ccgggctcgc agcagtgatg gaaccgggtg gcgggctcgt
     3661 cggcgagcat gcgggcgacc gcggcgccga tgatcggctg gtcgtcgatg agcagcaccg
     3721 acgcgccgcg ccgggtgcgg gtaggggggc tcatcggacg gtcgcccccg gccctccgat
     3781 cagctcggcg acggcggtga gcagctggtc gtcgtgaaag ctggtcttgt ccaggtagcg
     3841 atcggccccc gcggcgaggc cggcgcggcg atcttcttcg cgatccttgt acgagacgac
     3901 gacgatcggc gtgtgggcga ggcggggttc ggcgcgcatg cgggcgatca ggtcgtggcc
     3961 ggacagccgc ggcaggtcga catcggtgac caccagcgcg tagtcgccgg tgcgcaccgc
     4021 ggcccagccg tcgaccccgt cggcggcgag atcgacggcg tagccgcgct ggtcgagggc
     4081 gcggcgcagc gcctcgcgca ccgtcgtgga gtcgtcgatg accagcacgc gcttcttgcg
     4141 ggtgggctcg cgcaggcgcc gcaccggctg gaggcggctg ccggcgagca gcgcgttgac
     4201 cgagtgcacg aggtcatcga cgttgaggat gacgaccggg gaaccgtcct gcatgatcga
     4261 ggtggccgcg acatcggcga tcttgccgag ccgcgggtcg atcggctgga cgacgaggtc
     4321 gcgctcgccg aggaagcgat cgacggcgag gccgtaggtg tcgagctggt cgctgatcac
     4381 gaccacgcac agctcgtcgc gcgcttcgcc gggggcgagc tcgagcacct ggtgggcggc
     4441 gatcaggccg atgttctggc cgtcgagatc gaggtactgc cggccctcca ggctggagac
     4501 gcgcgccgcg gcgatctgga gcacgcgctc gacccggacc agcgggaacg cgaacggctc
     4561 gccggcgatc tcgaccagca gggtgcgcag gaccgagcgg gtgaccggca gctcgagctg
     4621 gaaggtcatg ccgcggccgg cctggggctc ggcgcgcagc ttgccgccga gctggtgcac
     4681 gacgtcgcgc acgacgtcga ggccgacccc gcgaccggag acgtcgccga cctcggccgc
     4741 ggtggtgaac ccgggctgga acaggaagtc ggtgagctcg gcctcggaca tgtggaagac
     4801 ccggtccgcg ggggcgaggc cctgctcggc gacgcggtgg cgcaccgccg cgaagtccac
     4861 gccgcggccg tc
//
LOCUS       DASVOR010001962        12443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_196224,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001962 DASVOR010000000
VERSION     DASVOR010001962.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12443
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196224"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VF516_36440"
     CDS             complement(<1..715)
                     /locus_tag="VF516_36440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113294.1"
                     /translation="MARPQVSDFLSRFVHPLVAGGELHIAAPIPLTDSERWEVELDGA
                     SLELTAVDDARTAVLSTLVCRPPALVLEGDDLALAVGLHNALFLVHPRAEEWSVSDRA
                     RRKVIDTALAQVSRPLTRERTRVMARHALLHNVFHLTRSDVTVSWWTGRARFQGQKPP
                     RRLTTWKGMRRVREDVTVVDFEELLAVPDTAPVVATLLRRTPLTQLLDSHPGAPPLHW
                     EDAVFLLRDAELARAIAYRL"
     gene            1030..2229
                     /locus_tag="VF516_36445"
     CDS             1030..2229
                     /locus_tag="VF516_36445"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEX8113295.1"
                     /translation="MKQVRSRVVLATLCCIGLPALAQPKPPPAEEPGWAKGRPKVGPG
                     MQMAPVPAFPIPTAPDKLPTSKFKLPPGFKVETWASGVLDARGLREGAKGNIFVSSLF
                     VANKVYVIPQQGDHKPKVIIDKLPYATGIEFHDGSLYVATNTKILRYDNVEDKLDNLG
                     EPKVLYDKLPGGNDHSWKYLRIRDNKLYYAVGAPCNLCDPGPWGKIFRMNLDGSGIET
                     IASGVRNTVGFDFDPKTGDLWFTDNGRDWFSEELPNDELNHVTKAGKEHFGYPFCHQG
                     NIPDPEFGWGKSCDDYVKPAALLGPHAAALGLTFYRGKMFPAKYQGAMFIARHGPWNR
                     TKKYADISVAWPDGKGEARVEAFMTGFVENNNYLGRPVDFLVLKDGSMLVSDDHAGAI
                     YRISYGK"
     gene            2299..3048
                     /locus_tag="VF516_36450"
     CDS             2299..3048
                     /locus_tag="VF516_36450"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8113296.1"
                     /translation="MQDQRKRLLLVVALMLGAFVVWSLLARPDAPVKQAQTTSQPSGS
                     AAGSAAAPAQPVPAKGATISLPPGATYAQRFAAVCAACHGANGRMETPLSPVLAGQPS
                     LYVITQLFLFREGRRSNEAMTAVAKTLTDDDLRGFSELIGTLPPVPAPPPATPPDPTR
                     MARGQALAQQHKCVFCHGADLGGGQQVPRIGGQHEDYLQASLGGFHSGSRPGYTQAMT
                     EAVSQIPPEDLDTLAYYVARFRGPAEALTGK"
     gene            complement(3133..4656)
                     /locus_tag="VF516_36455"
     CDS             complement(3133..4656)
                     /locus_tag="VF516_36455"
                     /inference="COORDINATES: protein motif:HMM:NF039751.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113297.1"
                     /translation="MALEHVAQGQTRFFTPGVAPDPRGILLGRFCVMTFPTLEGAVSW
                     FRLYSSEAALDELLPNLTITKCRTALGSREIVVQIPAVSSYAADRAARLCRLVGGAMY
                     TGTAKHFVKYRDDRSPYGYDAVDIGTMPATTDFMVHGDEFAQGYVREGELPFSRLLFR
                     LSIRKLPGGEKLETEDRGELYLAIARGLSEGIIRYLWRNRVEAQAGLFTPGATSAFDD
                     HARDRGYMLIRVHALPERILALFLGTPGIDVFRPAGATAAVAVGYEHPIDLASCASVF
                     PADTFHVFWPNDRVDVLPGPIALSDIADLTRVDLQMERPKEPRRHGGGPLEPIGVPLK
                     LAAARGPPRRVVATLIPLEHAARLKRILFALPPVSLRGHRVAATDRGILVIGSENLDV
                     IPLGQLLCELTPGLLVPLGMDVVPRVSAEVLARALGHQAGVVTVFTTDGKPFQVSEAT
                     LTSLERRSLAKLEVEQVAGLDYAAEPPAEAHVVNDAVGRFALWGFPDAPDRKLLPGK"
     gene            complement(5245..5790)
                     /locus_tag="VF516_36460"
     CDS             complement(5245..5790)
                     /locus_tag="VF516_36460"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEX8113298.1"
                     /translation="MATSSAKTVDEYLAALPADRRAALAAVRDTINKRIPRGYEHGML
                     YGGPAWYVPRSRLAETYNGQPLAVAVLASQKQYMALYLMTVYGDTKLNAWFRKAYAAT
                     GKKLDMGKSCIRFKTLDALPLDVVGEAVSKVSVDDYVKVYEKSRAGTKAGKKGTAVRA
                     AAAARKRAAKPARSPGKRGPG"
     gene            complement(5840..7717)
                     /locus_tag="VF516_36465"
     CDS             complement(5840..7717)
                     /locus_tag="VF516_36465"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEX8113299.1"
                     /translation="MNAESGEASRHTRLLPLAVSALGVVFGDIGTSPLYTLQMAGTLV
                     TGADDILGVLSLIFWGLTVVIGVKYLAFIMRADNRGEGGILALLALIPDGVKQLDGIT
                     LMIIAGAALLFGDGIITPSISVLSAIEGLRLAAPDAFSTGLIVAITLAILIGLFAIQR
                     RGTGAVGRLFGPVMVLWFATIGVLGLVQIVGNPGVLRALSPSYGVGYFARHGLQGFPI
                     LGVVVLCLTGGEALYADMGHFGARPIRVAWIALAMPALVLCYFGQGARVLADPAALAN
                     PFFALVPVGIWTYALVGLSAAATIIASQALITGVYSLTHQAIQLGLFPRVQIRHTSAH
                     AEGQIYVPAINWGLAIACCLLVIGFQESSRLAAAYGIAVSGTMAITSVAFYLVTRRTW
                     GWPRWKALPLLVLFLSFDVPFFAANLLKFVEGGYVPVLVGAAFFTVMVVWRLGRTALA
                     AYTRSHSAPLDQFLATLDQRVAARVPGTAIFLSSMASDIPMILEHHIQRIRALHERVV
                     LLTIVFAHVPYVPAAERVEVHELDKGLVRVIAHYGYMDETDIPAALAAAGPKCKLGID
                     LADATYYLGRETFLATAKGKLGPLSEQLFAFLSRNAASATSYFAIPSEQVIEIGTQID
                     L"
     gene            complement(7698..9557)
                     /locus_tag="VF516_36470"
     CDS             complement(7698..9557)
                     /locus_tag="VF516_36470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113300.1"
                     /translation="MSEAVDLGAENDEQMARLREALALGDGFQLVIVQVEPGERREEV
                     LRRLAGWSGKTGVPRLELVRLAQDESPVMRLAGGHAGVILVGLEADGPDRAERTRRMV
                     TELNWSRDRLPERVHGPLVLVVSQRVQSALFEQAPDFYSWRTHSTSIAPASHRTWRAF
                     PWLDGEPVEPDALRSMIAAGRSSRSLPELEIARLHERLGVALAVRGEFQDADAALATA
                     YQGYLRAGTTDDRVELLMLRATVERGRGRLDHAAEWLDRARRDATRGTPSPRLAARLL
                     SATALDSLDREDGASAETEIRLALRAAEAAGDDLELARLTYLRALLAFRSGDLKLAVA
                     GLEEALDGHARAGDVLGQALLLMALARAAVELGRSDDAERHAADAVARAQASRSADME
                     ARARACLAEIALANQRDGLAEEVLGKAVTPVDPLAVGHLAEARGQLALRRGELAAAER
                     FLQGALDAYQRRGTSWRIANVALELGKLGRRTQNWALAATGFETARRVGDAVQGAVAA
                     LGTAQVAYDQGERTPPLADRFAKAAQLLDAARQPTHADLARQHGGAVLSALGRDVEAR
                     KELETALAGFEARGQVELAAHARELLAGIEPRGTEPTDARTSSTVGADERRIR"
     gene            complement(9559..10881)
                     /locus_tag="VF516_36475"
     CDS             complement(9559..10881)
                     /locus_tag="VF516_36475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113301.1"
                     /translation="MPRPPIQDTLHLPALGRREVRAACDPSHPLIPEEQAQLHEDLSI
                     VQGGDRIRQVENRIRDAWADSYVCELVTGHAGCGKSTELLRLAAELRKPKEGRGYHVV
                     YVDAYDYLNPFEVRLPQLVVSLMAALAEEPRVDLRKTRSGPRLWDQIRALLTAAGREI
                     GSDLANEVPILKSLLRVDLKFVRDFRRESSDHIPQILGLIRDLVLEVRTQLPADQSEI
                     VFVVDNLEKIPDGETETKVSLHETLFLRELPLLDVPAHLVLTYPISLNYASVALRQVF
                     HDARQTTIPMVGIRQKPGAQVRGDDVQGIAALRRLLARRVRLDRVFADEGAIVAVVRE
                     SGGCVRDLLRLVGEMPTVGAIPFSSATVEAVIADYVNEYERILQGKPYLPLLHTIERT
                     GAFPGDTDQAWKQQLLLGLIVLEYDTGTWYDVHPLAKRTRAYRAATER"
     gene            complement(10953..11498)
                     /locus_tag="VF516_36480"
     CDS             complement(10953..11498)
                     /locus_tag="VF516_36480"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8113302.1"
                     /translation="MKTMTLLLVLAAAAGCGGDKPKVANPTPPVAAKPDTPPPETPPP
                     AVDKDTKVSPGIALSGDLAAMCNIKAPESANPKFDFDKDELTPEDRAVLDQLATCLTT
                     GAAKGKSVSLIGRADPRGTEEYNLGLGSRRASSVSQYLGRLGVGAGQMAVTTRGALEA
                     TGTDEAGWRNDRRVDVQLKTN"
     gene            11574..>12443
                     /locus_tag="VF516_36485"
     CDS             11574..>12443
                     /locus_tag="VF516_36485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113303.1"
                     /translation="MNRSIFASLILASLWCVLVLVMAGCGRKADAPAKAKEDAAPAAA
                     PLAMPALGVDQVRRFNYIYEAGSPAYDKAAAAYHKKDWPAVRAAAEQSLGKDPMHLGA
                     HRLLAAALAQTGEPAAAVDHLVTALAADYLQYAPTLAEDDLQSFMASPHGQAVAALAA
                     RIHDEYSRRIASGLWLVGRRSPFRWPRELGIQPSTSRGELYAFDRETRRYFRLSHTDH
                     QVVGFVRAAAGNEVALVGFDRIDRPKDARDDAAPLFARPWIQVYDAAQWKPATPRIHL
                     PAAREVALGYGAGD"
BASE COUNT         1880 a         4464 c         4338 g         1761 t
ORIGIN      
        1 ccagccggta ggcgatcgcg cgcgcgagct cggcgtcccg gagcaggaac accgcgtcct
       61 cccagtgcag cggcggcgcg ccggggtgcg agtcgagcag ctgggtcagc ggcgtgcggc
      121 ggagcagcgt cgcgaccacc ggggcggtgt cgggcaccgc gagcagctcc tcgaagtcga
      181 ccacggtgac gtcctcgcgg acccggcgca tgcctttcca cgtggtcagc cggcgcggcg
      241 gcttctggcc ctggaaccgc gcccgcccgg tccaccacga caccgtgacg tcgctgcggg
      301 tcaggtggaa cacgttgtgg agcagcgcgt ggcgcgccat cacccgcgtg cgctcgcggg
      361 tcagcggccg gctgacctgg gccagcgcgg tgtcgatcac cttgcggcgc gcgcggtcgc
      421 tgaccgacca ctcctcggcg cgcgggtgga ccaggaacag cgcgttgtgc agcccgaccg
      481 cgagcgccag atcgtcgccc tcgagcacca gcgccggcgg ccggcacacc agggtcgaca
      541 gcaccgcggt gcgcgcgtca tcgaccgcgg tcagctcgag gctggcgccg tcgagctcga
      601 cctcccagcg ctcggagtcg gtcagcggga tcggcgcggc gatgtggagc tccccgcccg
      661 cgaccagcgg gtggacgaac cgggacagga agtccgagac ctgcggccgc gccatctccg
      721 gccagcctag catggcgctc tcggcggcct cgccggcatg gcacaccgtg ccggcctggc
      781 tgccaggctc ggcgatgtgg acctcctggt cacggacgtc atgaccgggc ggatcactcg
      841 gctgctttga ggatcgagtc gtggcaccgg acggaatgcc acggacagtt gctcgggacg
      901 gcgaggacgg tgtaaccatc gcgttgcaat ggcccagccg agatgtcgac gctcgccggt
      961 cggacgctgt tacccagctc gtgggcttcc tgcagaatcg tcggcccaag acccccaagg
     1021 agacaagtca tgaagcaagt gcgctcgcgc gttgtgcttg ccacactctg ttgcatcggg
     1081 cttccggcac tggcccagcc aaagccgccg ccggcggaag agcccgggtg ggccaagggg
     1141 cgtccgaagg tgggacccgg catgcagatg gcgccggttc cggcgtttcc gattccgacc
     1201 gcacccgaca agctcccgac ctccaagttc aagttgccac ctggcttcaa ggtggagacc
     1261 tgggcttcgg gcgtgctgga cgcgcgcggg ttgcgcgagg gcgcgaaggg caacatcttc
     1321 gtcagttcgc tcttcgtcgc gaacaaggtc tacgtgatcc cgcagcaagg tgatcacaag
     1381 cccaaggtca tcatcgacaa gctgccgtac gccacgggca tagagttcca cgacggtagc
     1441 ctgtacgttg cgacgaacac gaagatcctg cggtacgata acgtcgagga caagctggac
     1501 aacctcggcg agcccaaggt gctgtacgac aagctgcccg gcggcaacga ccacagctgg
     1561 aagtacctgc gcatccgcga caacaagctg tattacgccg tcggcgcgcc gtgcaacctc
     1621 tgcgatccgg gcccgtgggg gaagatcttc cgcatgaacc tggatggcag cggcatcgaa
     1681 acgattgcct cgggcgtgcg caacacggtg ggcttcgact tcgacccgaa gaccggtgac
     1741 ctctggttca ccgacaacgg acgcgactgg ttcagcgagg agctgccgaa cgacgagctc
     1801 aatcacgtga ccaaggccgg caaggagcat ttcggctacc cgttctgcca ccagggcaac
     1861 atccccgacc cggaattcgg ctggggcaag tcgtgcgacg actatgtcaa gccggctgcc
     1921 ctcctgggtc cgcatgccgc cgccctgggc ctgacgttct atcgcggcaa gatgttcccg
     1981 gccaagtacc agggtgcgat gttcatcgcg cgccacggtc cgtggaaccg caccaagaag
     2041 tatgccgaca tctcggtggc ctggcccgac ggcaagggag aagcgcgggt cgaggccttc
     2101 atgaccggct tcgtcgagaa caacaactat ctcggtcgtc cggtggactt cctcgtgctc
     2161 aaggacggat cgatgctcgt gagcgacgat cacgcggggg ccatctaccg catcagctac
     2221 gggaagtgat gacgaggtgg actgcaggcc tgttgtcctg cgcggtggcg ctggcgctga
     2281 ccactgtcag gagcatggat gcaggaccaa cgtaagcgac tgttgctcgt ggtggcgctg
     2341 atgctgggcg cgttcgtggt gtggagcctg ctcgccaggc cggacgcgcc ggtgaagcag
     2401 gcgcagacca cgagccagcc gtccgggtcg gccgccggct cggctgctgc accggcccag
     2461 ccggttcccg ccaagggcgc gacgatctcg ctgccgcccg gggcgaccta cgcgcagcgc
     2521 ttcgccgccg tgtgcgcggc ttgtcatggc gccaacgggc gcatggaaac gccgctctca
     2581 ccggtgctgg ccggccagcc ctcgctgtat gtgatcacgc agctgttcct gtttcgcgag
     2641 ggccggcgca gcaacgaagc catgacggct gtggccaaga ccctgaccga cgacgacctg
     2701 cggggctttt ccgagctcat cgggaccctg ccacccgttc ctgccccgcc accggccaca
     2761 ccgcccgacc cgacccgcat ggccaggggg caggcgctgg cgcagcagca caagtgcgtg
     2821 ttctgtcacg gtgcggacct gggcggtggc cagcaggtgc cgcgcatagg aggccagcac
     2881 gaggattacc ttcaggcgag cctgggcggt ttccactcgg gctctcgccc aggatacacg
     2941 caggccatga cggaggcggt gagccagatc ccgccggaag acttggacac cctggcgtac
     3001 tacgtggcac ggttccgtgg ccctgcggaa gcactcaccg ggaagtagcg gcaggtcggc
     3061 gtactgcccg cccgcgtcgg cgaagacgtc gaggtcggtc aggaccggtg agcaaggcct
     3121 gcgcgctggc cgtcacttcc ccggcaagag cttgcggtcc ggcgcgtcgg ggaaccccca
     3181 cagcgcgaac cggccgaccg cgtcgttgac cacgtgggcc tcggcgggcg gctccgcggc
     3241 gtagtcgagc ccggcgacct gctcgacctc gagcttggcc agcgagcggc gctccagcga
     3301 ggtcagcgtg gcctcgctga cctggaacgg cttgccgtcg gtggtgaaca cggtgaccac
     3361 gccggcctgg tggccgagcg cgcgggccag cacctcggcc gagacccgcg gcacgacgtc
     3421 catcccgagc ggcacgagca ggcccggagt cagctcgcac aggagctgac cgagcgggat
     3481 cacgtcgagg ttctccgagc cgatcacgag gatcccgcga tcggtcgccg cgacccggtg
     3541 gccgcgcagc gacaccgggg gcagcgcgaa caggatccgc ttgagccgcg cggcgtgctc
     3601 gagcgggatc agcgtcgcga ccacccggcg cggcggcccc ctcgcggcgg cgagcttgag
     3661 cgggacgccg atcggctcga gcggcccacc gccgtggcgc cgcggctcct tggggcgctc
     3721 catctggagg tcgacccggg tcaggtcggc gatgtcgctc agcgcgatcg gcccgggcag
     3781 cacgtcgacc cggtcgttgg gccagaacac gtggaaggtg tcggccggga acaccgacgc
     3841 gcacgacgcc agatcgatcg ggtgctcgta gccgaccgcg accgcggcgg tggcgccggc
     3901 cgggcggaac acgtcgatgc cgggcgtgcc gaggaacagc gcgaggatcc gctcgggcag
     3961 cgcgtggacc cggatcagca tgtagccgcg atcgcgcgcg tggtcgtcga acgcgctggt
     4021 cgcccctggc gtgaacagcc cggcctgggc ctcgacccgg ttgcgccaca ggtagcggat
     4081 gatgccctcg gacagcccgc gggcgatcgc gaggtagagc tcgccacggt cctcggtctc
     4141 gagcttctcg ccgcccggca gcttgcggat cgacagccgg aacaacaggc gcgagaacgg
     4201 cagctcgccc tcgcggacgt agccctgggc gaactcgtcg ccgtgaacca tgaagtcggt
     4261 ggtcgccggc atcgtgccga tgtcgacggc gtcgtagccg tacggcgagc ggtcgtcgcg
     4321 gtacttcacg aagtgcttgg cggtgccggt gtacatcgcg ccgccgacca gccggcacag
     4381 ccgcgccgcg cggtcggccg cgtacgacga caccgccggg atctgcacga cgatctcgcg
     4441 gctgcccagc gcggtgcggc acttggtgat cgtgaggttg gggagcagct cgtcgagcgc
     4501 cgcctccgac gagtacagcc ggaaccacga caccgcgccc tccagcgtgg ggaacgtcat
     4561 gacacagaac cggccgagca ggatcccgcg gggatccggc gcgactcccg gcgtgaagaa
     4621 ccgggtctgc ccctgcgcga cgtgctccag cgccatcgcc ggcgagccta gcatggcgcc
     4681 gtgcggcacg acgccggcgg cccccccggg acgtcatcga tgatgtcatc cgcgataggc
     4741 ggcctgcggc tcgcggtcgg cgcccggccc tggtggtggc cgccgccgtc ggggccaacc
     4801 gccggatcgt gcgcaatctg acccgcggta cgcggcctgc acgagcgacc tggcaaccaa
     4861 cgaaaggctc acccatgcac gcattcgaag cgatcgatgt cctggagttc gtgacggtca
     4921 ccggcagctg atggcgacac cccgcctgcg aacaccgagg acaccggcgt ccggggcgcg
     4981 cagatcgccg accgttccct cgcgtgcgat ggcacgtgag cgagcgccgg ggcagcgtcg
     5041 ggccaaggtt ctcgaccacc tggccgtgac gccgcctcgg gtcttgacgc catctctacg
     5101 cctggaccgc ctgcgttgac gccatcgata cgccgaggcc gataggccta ggtgtcctgc
     5161 agaacctcgc gtcgcatctg ttcggcaggg cgcgagacct acgcgagcgg cagtggcggc
     5221 ggctcaacgc ctcgcggtcg cacctcaacc cggtccgcgc ttgccgggcg accgggctgg
     5281 cttcgccgcg cgtttccggg ctgctgcggc agccctgacc gccgttccct tcttgcccgc
     5341 cttcgtgccg gcgcgcgact tctcgtacac cttcacgtag tcgtcgaccg acaccttcga
     5401 taccgcctcg cccacgacat cgagcggcag cgcgtcgagt gtcttgaacc ggatgcacga
     5461 cttgcccatg tcgagcttct tgccggtcgc cgcgtacgcc tttcggaacc acgcattcag
     5521 cttggtatcg ccgtacaccg tcatcagata cagcgccatg tactgcttct gcgacgcgag
     5581 caccgcgacc gcgagcggct ggccgttgta ggtctcggcg agccgcgagc gcggcacata
     5641 ccacgcaggc ccgccgtaca gcatgccgtg ctcgtagccc ctggggatgc gcttgttgat
     5701 cgtgtcgcgg actgccgcga gcgccgcccg ccggtccgcc ggcagcgccg cgaggtactc
     5761 gtccaccgtc ttcgccgaac tggtcgccat cgtcgcctat catagcgatc tcgaacgagg
     5821 cctacctgtt ctaccgagat cacagatcga tctgcgtgcc gatctcgatc acctgctccg
     5881 acgggatcgc gaagtacgag gtcgcgctgg ccgcgttgcg ggacaggaac gcgaacagct
     5941 gctcgctgag cgggccgagc ttgcccttgg cggtcgccag gaacgtctcg cggccgaggt
     6001 agtacgtcgc atcggccagg tcgatcccga gcttgcactt cgggccagcg gcggcgagcg
     6061 ccgcggggat gtcggtctcg tccatgtaac cgtagtgggc gatcacgcgg accaggccct
     6121 tgtcgagctc gtggacctcg acccgctcgg ccgcggggac gtagggcacg tgggcgaaca
     6181 cgatggtgag cagcacgacg cgctcgtgca gcgcgcggat gcgctggatg tggtgctcga
     6241 ggatcatcgg gatatcgctg gccatcgagc tcaggaagat cgcggtgccc ggcacgcgcg
     6301 ccgcgacccg ctggtcgagc gtggcgagga actggtccag cggcgccgag tgcgaccggg
     6361 tgtacgcggc cagcgcggtc cggccgagcc gccacaccac catcacggtg aagaacgccg
     6421 cgccgaccag caccggcacg tagccgccct cgacgaactt cagcagattt gccgcgaaga
     6481 acggcacgtc gaacgacagg aacagcacca ggagcggcag cgccttccag cgcggccagc
     6541 cccaggtccg gcgggtcacc aggtaaaacg cgacgctggt gatcgccatc gtgccgctga
     6601 ccgcgatgcc gtacgccgcg gccagccggc tcgactcctg gaacccgatg accagcaggc
     6661 agcacgcgat cgccagcccc cagttgatcg ccggcacgta gatctggccc tcggcgtgcg
     6721 ccgaggtgtg gcggatctgg acccgcggga acagcccgag ctggatcgcc tggtgggtca
     6781 gcgagtacac gccggtgatc agcgcctgcg acgcgatgat cgtcgccgcc gccgacagcc
     6841 cgaccagcgc gtaggtccag atcccgaccg ggaccagcgc gaagaacggg ttggccagcg
     6901 ccgccggatc ggccagcacc cgcgcgccct ggccgaagta gcacagcacc agcgccggca
     6961 tcgccagcgc gatccacgcc acgcggatcg gccgcgcgcc gaagtgcccc atgtcggcgt
     7021 acagcgcctc gccgccggtc aggcacagca ccaccacccc gaggatcggg aagccctgca
     7081 ggccgtggcg cgcgaagtag cccaccccgt agctcggcga cagcgcgcgc agcacgcccg
     7141 ggttgccgac gatctggacg agaccgagca cgccgatcgt cgcgaaccac agcaccatca
     7201 ccggcccgaa cagccgcccg accgcgccgg tgccgcggcg ctggatcgcg aacagcccga
     7261 tcaggatcgc cagcgtgatc gcgacgatca gcccggtcga gaacgcgtcc ggcgccgcga
     7321 gccggagccc ctcgatcgcg ctcagcaccg agatcgacgg cgtgatgatg ccatcgccga
     7381 acagcagcgc cgcccccgcg atgatcatca gcgtgatccc gtccagctgc ttgacgccgt
     7441 cggggatcag cgcgagcagc gccaggatgc cgccctcgcc ccggttatcg gcgcgcatga
     7501 tgaatgcgag gtacttcacc ccgatcacca cggtcagccc ccagaagatc agcgacagca
     7561 cgccgaggat gtcgtccgcg ccggtcacca acgtccccgc catctgcagc gtgtacagcg
     7621 ggctggtccc gatgtcgcca aacaccaccc cgagcgcgct caccgcgagc ggcaacagcc
     7681 gcgtgtggcg gcttgcctca cctgattctg cgttcatccg cgccgaccgt actcgatgtg
     7741 cgcgcgtccg tgggttccgt gccgcgcggc tcgatgccgg ccagcagctc ccgcgcgtga
     7801 gccgccagct ccacctgacc gcgcgcctcg aaccccgcga gcgcggtctc gagctccttg
     7861 cgcgcctcga cgtctcgtcc gagcgcgctc aggaccgccc cgccgtgctg ccgtgccaga
     7921 tccgcgtgcg tgggctgcct ggcggcgtcc agcagctgcg cggccttcgc gaaccgatcc
     7981 gcgagcggcg gcgttcgttc cccttgatcg tacgccacct gcgccgtccc gagtgctgcc
     8041 acggcgccct gcacggcatc tccgacccgc cgcgcggtct cgaacccggt cgcagccagc
     8101 gcccagttct gggtgcgccg tcccagcttg ccgagctcca gcgcgacgtt ggcgatccgc
     8161 caggaggtcc cgcggcgctg gtaagcgtcc agggccccct gcaggaagcg ctcggccgcc
     8221 gcgagctcgc cgcgacggag cgcgagctgc ccccgcgctt cggccaggtg acccacggcc
     8281 agcgggtcca cgggagtcac ggccttgccg agcacctcct cggcaaggcc gtcgcgttga
     8341 ttcgccagcg cgatctcggc gagacaggcc cgcgcgcgtg cctccatgtc cgccgagcgg
     8401 ctggcctgcg cccgcgccac cgcgtcggcg gcatgacgtt cggcgtcgtc gctgcggccg
     8461 agctcgaccg cggcgcgggc gagtgccatc aggaggagcg cctgtccgag gacgtcgccg
     8521 gcgcgcgcat ggccgtcgag cgcctcctcc aggcccgcga ccgccagctt caggtcgccg
     8581 ctccggaacg ccagcagcgc ccgcagatag gtgagcctgg ccagctccag gtcgtctccg
     8641 gcggcctccg ccgcgcgcag ggcgagtcgg atctcggtct ccgccgacgc cccatcctct
     8701 cgatcgagcg agtccagggc ggtcgccgac aaaaggcgcg ccgcgagccg agggctcggc
     8761 gttccccggg tggcatcccg ccgtgcgcga tcgagccact ccgccgcgtg atcgagcctg
     8821 ccgcggccgc gctcgaccgt cgctcgcaac atcaacagct cgacgcgatc gtccgtggtc
     8881 ccggcgcgga ggtacccctg gtacgcggtc gcgagggccg cgtcggcgtc ctgaaactct
     8941 ccgcgcaccg ccagcgcaac gccgagccgc tcgtgcagtc gcgcgatctc gagctccggc
     9001 agcgaccgcg acgatctccc cgccgcgatc atcgaccgca gcgcatcggg ttccaccggc
     9061 tcgccatcga gccacgggaa cgcccgccag gtgcggtggg acgccggcgc gatgctcgtc
     9121 gagtgggtgc gccagctgta gaagtcgggc gcctgctcga acagcgcgct ctggacccgc
     9181 tgcgagacga ccagcacgag cgggccgtgg acccgctcgg gcagccgatc gcggctccag
     9241 ttgagctcgg tcaccatccg ccgcgtgcgc tcggcgcggt cggggccgtc cgcctccagc
     9301 ccgaccagga tcacgcccgc atgcccaccg gcgagccgca tcacgggaga ttcgtcctgc
     9361 gcgaggcgca ccagctccag gcgcggcacg ccggtcttgc ccgaccagcc tgcgagccgc
     9421 cgcagcacct cctcgcgccg ctcgccgggc tcgacctgca cgatcacgag ctggaagccg
     9481 tcgccgagcg ccagcgcctc gcgcagccgg gccatctgct cgtcgttctc cgcgccgaga
     9541 tcgacggcct cgctcatctc agcgctccgt cgcggcgcgg taggcccgcg tgcgcttggc
     9601 cagcggatgg acgtcgtacc aggtgccggt gtcgtattcg aggacgatca gcccgagcag
     9661 cagctgctgc ttccatgcct ggtcggtgtc gccggggaac gcgccggtgc gctcgatggt
     9721 gtggagcagc ggcaagtacg gcttgccctg caggatccgc tcgtactcgt tgacgtagtc
     9781 cgcgatcacc gcctcgacgg tcgcgctcga gaacgggatg gcgccgacgg tcggcatctc
     9841 gccgaccagg cgcagcaggt cgcgcacgca gccgccgctc tcgcggacca cggccacgat
     9901 cgcgccctcg tcggcaaaca cccggtcgag ccggactcgc cgcgcgagca gccggcgcag
     9961 cgccgcgatc ccctgcacgt cgtcgccgcg cacctgcgct cccggcttct ggcggatccc
    10021 caccatcggg atcgtggtct gccgcgcgtc gtggaacacc tgccggagcg cgaccgacgc
    10081 gtagttgagc gagatcgggt aggtcaggac gaggtgcgcc ggcacgtcga gcagcggcag
    10141 ctcgcgcagg aacagggtct cgtgcaagga caccttggtc tcggtctcgc cgtcggggat
    10201 cttctcgagg ttgtcgacga cgaacacgat ctcgctctgg tcggccggga gctgggtccg
    10261 gacctccagc acgagatcgc ggatcagccc gaggatctgc gggatgtgat cgctcgactc
    10321 ccggcggaag tcgcgcacga acttcagatc gacccggagc agcgacttga ggatcgggac
    10381 ctcgttcgcc agatcgctgc cgatctcgcg ccccgccgcg gtgagcaagg cgcggatctg
    10441 gtcccacagc ctgggcccgc tccgcgtctt tcgcagatcg acgcgcggct cctcggcgag
    10501 cgcggccatc agcgacacca cgagctgggg cagccggacc tcgaacgggt tcaggtagtc
    10561 gtaagcgtcg acgtagacca cgtggtaccc gcggccctcc ttgggcttgc gcagctcggc
    10621 ggcgaggcgc agcagctcgg tggacttgcc gcagccggcg tggccggtga cgagctcgca
    10681 gacgtacgaa tcggcccacg cgtcgcggat ccggttctcg acctgccgga tccggtcgcc
    10741 gccttgcacg atcgacaggt cctcgtgaag ctgcgcctgc tcctcgggga tcagcgggtg
    10801 cgatggatcg cacgccgcgc ggacctcgcg ccggcccagc gcggggaggt gcagcgtgtc
    10861 ctggatcggc gggcgtggca ccgatgcagc gtatcaaagg tcctcggctc cgtcgcgcgc
    10921 gactcaccgg gcggccggga ccccagccgc gatcagttgg tcttgagctg gacgtcgacc
    10981 cggcgatcgt tgcgccagcc ggcctcgtcg gtgccggtcg cttccagggc gccgcgcgtg
    11041 gtgaccgcca tctggcccgc gccgacgccg agccgcccca ggtactggct gaccgagctg
    11101 gcgcggcgcg agccgagtcc gagattgtac tcctccgtgc cgcgcggatc ggcgcggccg
    11161 atcagcgaca ccgacttgcc cttggcggcg cccgtggtca ggcaggtggc gagctgatcg
    11221 agcacggcgc ggtcctcggg ggtcagctcg tccttgtcga agtcgaactt ggggttggcc
    11281 gactcgggcg ccttgatgtt gcacatcgcc gcgagatcgc ccgacagcgc gatgcccgga
    11341 ctgaccttgg tgtccttgtc gacggccggg ggcggcgtct cgggcggggg ggtgtcgggc
    11401 ttggcggcga ccggcggcgt gggattggcg accttgggct tgtcgccacc gcagcccgcg
    11461 gccgcggcga ggacgagaag gagggtcatg gtcttcatgc gacgcatcct gacacagaaa
    11521 actgcaccgt cacgcgatct cgcaatcgag tcgcgctaga tcgatgaggt cccatgaatc
    11581 ggtcgatctt cgcctcgctg atcctcgcct cgctgtggtg tgtgctcgtg ctcgtgatgg
    11641 ccggatgcgg gcgcaaggcc gacgccccgg ccaaggccaa ggaggacgcg gcgccagcgg
    11701 ccgcgccgct cgcgatgccc gcgctcgggg tcgatcaggt ccgccggttc aattacatct
    11761 acgaggccgg atcgccggcg tacgacaagg cggccgcggc gtatcacaag aaggactggc
    11821 ccgcggtgcg cgcggcggcc gagcagtcgc tcggcaagga tccgatgcac ctcggggcgc
    11881 accggctgct cgcggccgcg ctggcccaga ccggcgagcc ggcggcggcg gtcgatcacc
    11941 tggtgaccgc gctcgcggcg gactacctgc agtacgcgcc gacgctggcg gaggacgatc
    12001 tgcagagctt catggcctcg ccgcacggcc aggcggtcgc ggcgctcgcc gcgcggatcc
    12061 acgacgagta cagccggcgg atcgcgagcg ggctgtggct ggtcggccgg cgcagcccgt
    12121 tccgctggcc cagggagctc gggatccagc cgtcgaccag ccgcggcgag ctgtacgcgt
    12181 tcgaccgcga gacccgccgg tacttccggc tcagccacac cgaccaccag gtcgtcggct
    12241 tcgtgcgcgc ggcggcgggc aacgaggtcg cgctcgtcgg gttcgaccga atcgaccggc
    12301 ccaaggacgc cagggacgac gccgcgccgc tgtttgcgcg gccgtggatc caggtctacg
    12361 acgccgccca gtggaagccg gcgacgccgc ggatccacct gcccgccgcc cgcgaggtcg
    12421 cgctcggcta cggggcgggc gat
//
LOCUS       DASVOR010001963         4557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_166593,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001963 DASVOR010000000
VERSION     DASVOR010001963.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166593"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(230..1072)
                     /locus_tag="VF516_36490"
     CDS             complement(230..1072)
                     /locus_tag="VF516_36490"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEX8113304.1"
                     /translation="MPTWQLRVGAVQLCSSNDLAANLARCRELTAQAAAEGAQLVVLP
                     ECFSFLGRAEGEKLRHAESFDDPAAAGPVLGTLRELAARHGVWIVGGGTPEQVPGDAR
                     RTYNTALVVDPRGEIAARYRKIHLFDVDIPGGAVLRESDSTARGNEPVVVDVAGAPVG
                     VSICYDVRFPELYRRLVKDLGAEVLLVPAAFTAHTGGAHWHLLLRARAIEDQAWVVAP
                     AQWGRHSEKRESYGHTLIADPWGTVVGERAEGDGVVIAALDGETVARRRTQMPCLQHA
                     VLWR"
     gene            1310..2041
                     /gene="rimP"
                     /locus_tag="VF516_36495"
     CDS             1310..2041
                     /gene="rimP"
                     /locus_tag="VF516_36495"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEX8113305.1"
                     /translation="MSRNPLQQRLISIVEPVCQAAGYELVELRFVLEQGGWTLRVAID
                     RPLDEHVELDEIPTDRVDLIDCENLSRELSAVLDVEDPIPQAFHLEVGSPGIDRPLRT
                     AAHFLHFVGSEAKIQLAAPLHLPAGERRNFKGILQGVEDGHVLIECDGTAFRLPIDDI
                     DHAKLVPDWDAVMKGKSGVGKPSARPVKPGHRPSKPGPSQKRAAGERPQRPRDRQNER
                     QIEHPDSDGRDGKMDPAAASNKEQG"
     gene            2044..3621
                     /gene="nusA"
                     /locus_tag="VF516_36500"
     CDS             2044..3621
                     /gene="nusA"
                     /locus_tag="VF516_36500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEX8113306.1"
                     /translation="MEAKLDMVLEQVGRDKNIDKSVLINALEQAILTAAKRTFGMNRE
                     MEAKFNPETGSVDLFLIVNVVEDDDAIEGREITVAQAHKAGLEAELGDELLFQVFYRS
                     EDDDRAREQDEKFGDLIDLKNASKKFGRIAAQTAKQVIYQRVREAERDNVYNEFKDRK
                     GEIISGIVRRFERGALVIDIGKAEAVLPMREQVPRESYRVGDTIKAYCLDIDRNARGP
                     QVILSRTHKGLLEKLFEQEVPEIYEKIVRIESSAREPGARAKIAVSSRDRDVDPVGAC
                     VGMKGSRVQAVVQELRGEKIDIVPYDDDPARFVCNAIAPAEVSRVIIDAEGHRMELIV
                     PDDKLSLAIGKKGQNVRLASQLTGWRIDIHSESKIYELERRAKEQIAQAIDTAGFDTA
                     VADTLFKLGWRSVGELGRAHVEELAGLPGIGGADGARRIIEAAQHYMASGGRRRDDAR
                     KEAERRAHMSGTQQLMELDGIDEDVLNVLAGANIHSPEDLIKTPIEAIATSTGIDMGD
                     LARLRQRAISWLSEVSS"
     gene            4162..>4557
                     /locus_tag="VF516_36505"
     CDS             4162..>4557
                     /locus_tag="VF516_36505"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEX8113307.1"
                     /translation="MRVYEIAKEVGIPNKDLIAKIRALGLEVNNHMSSLDADDVARIK
                     RSLEKERQSVAQPQQTQRLAPGGTVLRRRSSGERPAESPSDDPVIRRRASGESPADDH
                     SGEREPMRREPERHAAAPAGTPTSASAGGP"
BASE COUNT          762 a         1597 c         1601 g          597 t
ORIGIN      
        1 cgatgaagcc ctgggcgtcg cgcggctcgg tcacgccccg gccgaccagg cagcgcgcgg
       61 tcgcggggcg caggcgcagc gcgccggcga gcttctggac gagggcttcg tcgacatggc
      121 gcaggcgcag ttgcacgccc cgcacgctag tggtggggtc tgacagcaaa ccgcgtgaga
      181 tccctggctt cggcgcgatc ccgggatcgc tcgcgccgcc ggcgagcggc tatcgccaca
      241 gcacggcgtg ctgaaggcac ggcatctggg tccgccggcg agcgaccgtc tcgccgtcga
      301 gcgcggcgat cacgacgccg tcgccctcgg cgcgctcgcc gaccaccgtg ccccacggat
      361 cggcgatcag cgtgtggccg tagctctcgc gcttctcgct gtggcggccc cactgcgccg
      421 gcgcgacgac ccaggcctgg tcctcgatcg cgcgggcgcg cagcaggagg tgccagtgag
      481 cgccgccggt gtgggcggtg aacgccgcgg gcaccagcag gacctcggcg cccaggtcct
      541 tgaccagccg ccggtacagc tccgggaacc gcacgtcgta gcagatcgag accccgaccg
      601 gcgccccggc gacgtcgacc acgaccggct cgttgccccg cgcggtcgag tccgactcgc
      661 gcagcacggc gccgcccggg atgtcgacgt cgaacaggtg gatcttgcgg tagcgcgccg
      721 cgatctcgcc gcgcgggtcg accaccagcg ccgtgttgta ggtccgccgg gcatcgccgg
      781 gcacctgctc gggcgtgccg ccgccgacga tccagacccc gtggcgggcc gcgagctcgc
      841 gcagcgtgcc cagcaccggg ccggcggcgg ccggatcatc gaagctctcg gcatggcgta
      901 gcttttcgcc ctcggcccgg cccaggaacg agaagcactc ggggagcacc acgagctggg
      961 cgccctcggc cgccgcctgg gcggtcagct cgcggcatct cgcgaggttg gccgccaggt
     1021 cattggacga gcacagctgg accgcgccga cgcgaagctg ccaggtgggc atgtctgtta
     1081 ggtaccacag cggaactacg agagatcggt cttcggctac gtgctgaaat ccacttgctc
     1141 ctcgatccca gcagtggtac ccaatccgac agcttcaatc gctctgcggt gaggtggccc
     1201 ttcggccacc ttttttttcg gttccggttc ggccctcggt tcagttccag ttcggtccca
     1261 gtttcgtcca accccctctc gtttccagtc tccgtcccga tcttccgcca tgtccagaaa
     1321 ccctctccag caacgcttga tctcgatcgt cgagccggtc tgccaggccg ccggctacga
     1381 gctggtcgag ctgcgcttcg tgctggagca gggcgggtgg acgctgcggg tcgcgatcga
     1441 ccggccgctc gacgagcacg tcgagctcga cgagatcccg accgaccgcg tcgacctgat
     1501 cgactgcgag aacctgtcgc gcgagctgtc ggcggtgctc gacgtcgagg acccgatccc
     1561 gcaggcgttc cacctcgagg tcggctcgcc gggcatcgac cggccgctgc gcaccgccgc
     1621 ccacttcctg cacttcgtcg gcagcgaggc caagatccag ctcgcggcgc cgctccacct
     1681 gccggccggc gaacgccgca acttcaaggg gatcctgcaa ggcgtcgagg acggccatgt
     1741 cctgatcgag tgcgacggga ccgcgttccg cctgccgatc gacgacatcg accacgccaa
     1801 gctggtcccg gactgggacg cggtcatgaa gggcaagagc ggggtcggca agccgtccgc
     1861 caggccggtc aagcccggtc atcgcccgag caaaccgggg ccgagccaga agcgggccgc
     1921 cggcgagcgg ccgcagcgcc cgcgcgaccg ccagaacgag cggcagatcg agcatccaga
     1981 cagcgacgga cgggacggga agatggaccc cgccgctgcc agcaacaagg aacagggata
     2041 gccatggaag cgaaactgga catggtcctg gagcaagtcg gccgggacaa gaacatcgac
     2101 aagagcgtgc tgatcaacgc gctcgagcag gccatcctga ccgccgcgaa gcgcacgttc
     2161 ggcatgaacc gggagatgga ggcgaagttc aaccccgaga ccggctcggt cgacctgttc
     2221 ctgatcgtca acgtggtcga ggacgacgac gcgatcgagg gccgcgagat cacggtcgcc
     2281 caggcccaca aggcgggcct cgaggccgag ctcggcgacg agctgctgtt ccaggtgttc
     2341 taccgctccg aggacgacga ccgggcgcgc gagcaggacg agaagttcgg cgacctgatc
     2401 gacctcaaga acgcatcgaa gaagttcggc cggatcgccg cgcagaccgc caagcaggtg
     2461 atctaccagc gggtccgcga ggccgagcgc gacaacgtct acaacgagtt caaggaccgc
     2521 aagggcgaga tcatctcggg catcgtgcgc cggttcgagc gcggcgcgct ggtgatcgac
     2581 atcggcaagg ccgaggcggt gctgccgatg cgcgagcagg tgccgcgcga gagctaccgg
     2641 gtcggcgaca cgatcaaggc gtactgcctg gacatcgacc gcaacgcgcg cgggccgcag
     2701 gtcatcctgt cgcgcaccca caagggcctg ctcgagaagc tgttcgagca ggaggtcccc
     2761 gagatctacg agaagatcgt gcggatcgag tcgagcgccc gcgagcccgg ggcgcgcgcc
     2821 aagatcgcgg tgtcgtcgcg cgaccgcgac gtcgacccgg tcggcgcgtg cgtcggcatg
     2881 aagggcagcc gggtccaggc ggtggtccag gagctgcgcg gcgagaagat cgacatcgtg
     2941 ccgtacgacg acgatccggc gcggttcgtg tgcaacgcga tcgcgccggc cgaggtgtcg
     3001 cgcgtgatca tcgacgccga gggccaccgc atggagctga tcgtccccga cgacaagctg
     3061 tcgctggcga tcggcaagaa gggccagaac gtccggctgg cgtcgcagct caccggctgg
     3121 cggatcgaca tccacagcga gtcgaagatc tacgagctcg agcgccgcgc caaggagcag
     3181 atcgcccagg cgatcgacac ggccgggttc gacaccgcgg tcgccgacac gctgttcaag
     3241 ctcggctggc gctcggtcgg cgagctcggc cgcgcccacg tcgaggagct cgccgggctg
     3301 cccgggatcg gcggggccga cggcgcgcgc cggatcatcg aggcggccca gcactacatg
     3361 gcgtcgggcg gccggcggcg cgacgacgcg cgcaaggaag ccgagcggcg cgcgcacatg
     3421 tcggggaccc agcagctcat ggagctcgac ggcatcgacg aggacgtgct caacgtgctc
     3481 gccggcgcga acatccattc gcccgaggac ctgatcaaga cgccgatcga ggcgatcgcg
     3541 acgtcgaccg ggatcgacat gggcgacctc gcgcggctcc gccagcgcgc gatcagctgg
     3601 ctctcggagg tgtcgtcctg accggccacg cttcacctca cgttcccatc cgcacctgca
     3661 ccggctgccg gaagaccgcg ccgcagcacg agctggtgcg gctggtgttc gagcgcagcg
     3721 agacgggggg cagagccccc ggtggggacg atggcatgcg gctggtgatc gacccgtcta
     3781 cctggccgtc tacctggtcc ccggctggcc ggccggtcac cgggcccgtc gcgccgcctg
     3841 ccgaggcgcc ggcggtgccg cccggcgagg cacctgccgc gcggcgcgtc gatcggcccg
     3901 ggcgcctgcc cggacgcggg gcctacgttc accccgaccc ggcctgcgtg acggcggccg
     3961 ggctatcccg aagctttcgc agacccgtcc gtgcggccga tgtggcgcag atcgtgtccc
     4021 aagtgtcacc gatccacgac aatctggggg atcaacccat ccaaaacgct cgtcaaaacg
     4081 cccctggcct tgcggatgga gacactgtag aaatgccccc tcggatcaag gcgacgaaga
     4141 agaaggacga ccggagcgaa gatgcgcgtc tatgagattg cgaaagaggt ggggatcccc
     4201 aacaaggacc tcatcgccaa gatccgagcg ctcggtctcg aagtgaacaa ccatatgtcc
     4261 tcactcgatg ccgacgacgt tgctcgcatc aagcgctcgc tggagaagga gcggcagtcg
     4321 gtggcacagc cccagcaaac ccagcgcctg gcccccgggg gcaccgtgct ccgccggcgc
     4381 tcgagtggtg agcggccggc cgagtcaccg tcggacgacc cggtgatccg ccggcgagcg
     4441 agcggtgagt caccggcgga cgaccacagc ggcgaacgcg agccgatgcg gcgcgagccc
     4501 gagcggcacg ccgccgcgcc ggccgggacg ccgacctcgg ccagcgccgg cgggccc
//
LOCUS       DASVOR010001964         3162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488435,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001964 DASVOR010000000
VERSION     DASVOR010001964.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3162
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488435"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1598..2251
                     /locus_tag="VF516_36510"
     CDS             1598..2251
                     /locus_tag="VF516_36510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113308.1"
                     /translation="MIDRAVLRRYARQVQRREDPKTSWDGEEALWIVAPHVPAILSER
                     RTLDLVATGVYRVGPSPFGFLWIAANELPLADELIPFLIARSGRPLDAFVGWVKTRRP
                     IEWLLRVLEYLPMSTAAHEDLRSYVFPKTDDPVVRARQRMIAEWAVESSPEMRDKLVG
                     EGRMEEARSSLRHVLALRGLALSSEEEARIDACGDLDTLRRWHDQAVVVASAAEALQ"
     gene            2500..2937
                     /locus_tag="VF516_36515"
     CDS             2500..2937
                     /locus_tag="VF516_36515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113309.1"
                     /translation="MRRIAICLALASTGGCTAPSPAATPPAREAAAAPARTDALASVV
                     GVWRGTSLCTVRPSPCNDETVVYYVSGTDRDHLLWVANKIVDGKEVEMGRSECQLVPA
                     EQRVVCRLERGAFLFAIDGNRMHGRLDRLDGTRYRVGEVERVR"
BASE COUNT          546 a         1003 c         1112 g          501 t
ORIGIN      
        1 gtcctggaag cagctgttcg accctgcgcc gtcgacgatt cgcctcgagc tcggcgacga
       61 ccggatccag ccgcccgcgc ccccgccggc ctccgacgac cgtcagaagt cccgctcgta
      121 agtccacgtc cggctcacaa ccggcgccac cagactcatg gagaattgct gccctcgcga
      181 gggacgggga gaatcacgat catgaagacg agcccgaagg tcggtcaggt gttgcaaggg
      241 tactttcgtg tcgagcagtt tcgcagcacg ggaaacaagg ccaccggcat tccatccatg
      301 ccctcaggac gctcgactcc gcttccgcca cggccagacc tgttaccagc tcccatgcgg
      361 agtgcggcag tgcaatcgaa gtcagcgtgg agcgggtccg gaaggccact gcctgaccag
      421 ttaccagtac agatgcgacg gatggtcggt caacccaagt ccgagcgcga ggcaggcccg
      481 ccgtggagac gggcaccgcg gccggagttg ctgccgggaa tgggcccagc ggtcgtgggc
      541 gggctgcgcg tggtggcgca ggctcgatcg aacaaaggcg tctccgcgac gccgctctcc
      601 gcaagtcatc tgcgagtgat cggcgaaggg cggccgctcg aaccgaagat ccggcagacc
      661 atggaggcgt tctttcaggc cgatttctcc agcgtgcgcg tgcacgaagg gccgacggga
      721 catgcgatgg gggcgctcgc gttcacgctg ggcgaagatc ttcacttcgc accaggattg
      781 tatgaccctg cgagccgcgc aggaatcgag ctcctcggac acgagttgac gcatgtcgtg
      841 cagcaacggg aagggtgggt ggtcaatccg ttcgggacgg gtgttgcgat cgtgcaggat
      901 ccagcgctgg aggccgaggc cgaccggctt gggcagaaga tcgctcgcga gatctggcta
      961 cgtccgggtg tcgcaccggc ggcggagata cgtggtgcag tggaaaagag acgatacccc
     1021 caacgtgggc aggctctacc cactgtatcc ggactggctg atcccgtgat gcagccttct
     1081 ttgcggcacg cgagcttcat agaccagatg aagtcggcgt ttcgcatcta tggcggtcac
     1141 tctgccaaaa cggcagtgcg ggttgcgcag cgaatggaga ggcagccggt tccagaacca
     1201 ccggaaagca agaacttcag gattgtgccg gttccctatg atcatacaga ggcaaagctc
     1261 gcgatagaat cgcgcaagaa gtcgaaggaa ccacggttgg gcaaggactg ggccattgtt
     1321 cagggttaca atggtgaatc cgactacgct acacccgtgt tttctgcggc cagctctccg
     1381 ggcggaagcg cgaagatgca cgcggaacgg gccgtgatag agaaactctg caagcacgta
     1441 ggcgccgaat ttagccagtc ggatggcgcc aagaccgcga agtcgctacg cgcacgtaac
     1501 attagtcata tcgtgaagtg gtatacggaa gttcctccgt gcccaaagtg caagaaatgg
     1561 tgccaggcgt ttgccgcgga atatctctct gacgtagttg atcgaccgcg cggtgctgcg
     1621 gcgctacgcc cgtcaggtgc agcgccgcga ggatcccaag acgtcctggg acggcgagga
     1681 ggcactttgg atcgtggcgc cccacgtgcc ggcgatcctc tcggagcggc gtacgctcga
     1741 tctggtggcg accggcgtct accgtgtggg cccttcgccc ttcgggttcc tgtggatcgc
     1801 cgcgaacgag ctgccgctcg ccgacgagct gatcccgttt ctgatcgcac gctcgggccg
     1861 gccgctcgac gcgttcgtcg gctgggtcaa gacacgccgg ccgatcgagt ggctgctgcg
     1921 cgtgctagaa tacctaccga tgagcaccgc ggcccatgaa gacctcagaa gctacgtgtt
     1981 ccccaagacc gacgatcccg tcgtccgtgc tcgccagcga atgatcgccg agtgggcggt
     2041 ggagtcttcg ccggagatgc gcgacaagct ggtcggggag gggcgcatgg aggaggcacg
     2101 ctcgagcctc cgtcacgtgc tcgcgctgcg cgggctcgcg ctgtcctcgg aggaggaggc
     2161 gcggatcgac gcgtgcggcg atctcgacac cctgcgccgc tggcacgacc aggcggtcgt
     2221 cgtcgcgagc gccgctgagg cgctgcagta ggcctgatga cccgtacgcg cctgggcgct
     2281 tgagcgcgtc gggcattgtg ccgtcgggcc gggaagcagt ggagcgggcg ggtcgtgcgg
     2341 ccagcagggc ggccaggccg ttgctcgcag cccgggcccg cggaggatcc accaaccgcc
     2401 ggggccgcgt cgttggggcg cggacggacg agccaccggg tggcaagatc agggggcatg
     2461 ggcatcgctc cacatgtcgg gaaactgtat cgtggtggta tgaggcgaat cgcgatctgc
     2521 ctggcgctcg caagcaccgg cggttgcact gcgcccagcc cggccgccac gccgccggcg
     2581 cgcgaggcag ccgctgcgcc ggcacggacg gacgctctgg cgtcggtcgt cggcgtctgg
     2641 cgcggcacgt cgctgtgcac ggtgcgcccg agcccctgca acgacgagac cgtcgtgtac
     2701 tacgtctcgg gcaccgaccg cgaccacctc ctgtgggtcg cgaacaagat cgtcgatggc
     2761 aaggaggtgg agatggggag atccgaatgt cagctcgtgc ccgccgagca gcgggtcgtg
     2821 tgccggttgg agcgcggcgc cttcctgttc gcgatcgacg gcaaccggat gcacggccgg
     2881 ctcgatcggc tggacggtac gcgctaccgc gtgggcgagg tcgaacgcgt gcgctgagcc
     2941 ccccctggcc ggagctccgc gtcgccgagc gcatccgcga aattggcgac tgcgggcgcg
     3001 cccagctcgc agcgacgcgt ccggccaccg aaaactggat ctcgccggcc gctcgtgtag
     3061 gctgaagtgc atgggcgctc ccgccgggcc agctcctccc gcagcggttc aggccgccgc
     3121 gcgtccgccg gaccggcgcg cgcaggccgg atccgcgccg gg
//
LOCUS       DASVOR010001965         1765 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_50333,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001965 DASVOR010000000
VERSION     DASVOR010001965.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50333"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1689
                     /locus_tag="VF516_36520"
     CDS             127..1689
                     /locus_tag="VF516_36520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8113310.1"
                     /translation="MTDTPPSQTPERRSRENQDDQREREALERRSRENQDDQRASAAL
                     EPILIVDDEKNIRRTLRMVLEGEGHVVHEAGSIAEASAVLERDPIDLILLDVKLGDDN
                     GLDLLRTLKARGEDGMSSRTSEIPVVMISGHATIEDAVAATRLGAFDFMEKPLDRNRV
                     MVTARNALERRRMWREVHDLRRAVDARWELLGGTPVMGDLRRQIAKVAPTRSRVLITG
                     DSGTGKELIARAIHRNSSVSAGPFVKVNCAAIPPELIESELFGHERGAFTGAIAKKRG
                     LFEVADGGTIFLDEIGDMAPSAQAKVLRVLQTGEFTRVGGEKSLRTECRVVAATNRDL
                     EQMVKDGTFREDLYFRLNVVPIRSPDLRERVDDIPLLVESFVRECCDENGLGYKPIDE
                     PVFDRLKLYDWPGNVRELRNVVERLVIMSDDVIREKDLPPYLGGPRPPGQTRTTGPQT
                     AIDLGRYAGKSLREFREEVESEFIRIRLAEFDWNISRTAQALGIERTNLHKKLRALGI
                     HRGEGSAGGDHD"
BASE COUNT          295 a          645 c          618 g          207 t
ORIGIN      
        1 cagcgatgcc gacgccgatg ccgacgacct gcacgccgcc gcacgcggcg atggaccggc
       61 gagcgcagcg accgaccccg ccgccgaccc caccgcgccc gacagcccgc cacggaactc
      121 cagcccatga ccgacacccc acccagccag acccccgagc ggcgaagccg cgagaaccag
      181 gatgaccagc gcgagcgcga agcgctcgag cggcgaagcc gcgagaacca ggatgaccag
      241 cgagcgagcg cagcgctcga gcccatcctg atcgtcgacg acgagaagaa cattcgccgg
      301 accctgcgca tggtcctcga gggcgagggc cacgtcgtcc acgaggccgg ctcgatcgcg
      361 gaggccagcg cggtgctcga gcgcgacccg atcgacctga tcctgctcga cgtcaagctc
      421 ggcgacgaca acggcctcga cctgttgcgc acgctcaagg cgcgcggcga ggacggcatg
      481 tcgtcgcgga cctccgagat cccggtcgtg atgatctccg gacacgcgac gatcgaggac
      541 gcggtggcgg cgacccggct cggcgcgttc gacttcatgg agaagccgct cgaccgcaac
      601 cgcgtgatgg tgaccgcgcg caacgcgctg gagcgccgcc ggatgtggcg cgaggtccac
      661 gacctccgcc gcgcggtcga cgcgcgctgg gagctgctcg gcgggacgcc ggtcatgggg
      721 gacctgcggc gccagatcgc caaggtcgcg ccgacccgca gccgggtgct gatcaccggc
      781 gacagcggca ccggcaagga gctgatcgcg cgcgcgatcc accgcaacag cagcgtgtcg
      841 gcggggccgt tcgtcaaggt caactgcgcc gcgatcccgc ccgagctgat cgagagcgag
      901 ctgttcggcc atgagcgcgg cgcgttcacc ggtgcgatcg ccaagaagcg cggcctgttc
      961 gaggtcgccg acggcggcac gatcttcctc gacgagatcg gcgacatggc gccgtccgcc
     1021 caggccaagg tgctgcgcgt gctgcagacc ggcgagttca cccgggtcgg cggcgagaag
     1081 agcctgcgca ccgagtgccg ggtggtcgcc gcgaccaacc gcgacctcga gcagatggtc
     1141 aaggacggca cgttccgcga ggacctgtac ttccggctca acgtcgtgcc gatccgctcg
     1201 ccggacctgc gcgagcgcgt cgacgacatc ccgctgctgg tcgagtcgtt cgtccgcgag
     1261 tgctgcgacg agaacggcct gggctacaag ccgatcgacg agccggtgtt cgaccggctc
     1321 aagctctacg actggccggg caacgtgcgc gagctgcgca acgtggtcga gcgcctggtg
     1381 atcatgtccg acgacgtgat ccgcgagaag gacctgccgc cgtacctcgg cggcccgcgc
     1441 ccaccgggcc agacccgcac caccgggccg cagaccgcga tcgacctcgg ccgctacgcc
     1501 ggcaagagcc tgcgcgagtt tcgcgaggag gtcgagtccg agttcatccg gatccggctc
     1561 gccgagttcg actggaacat ctcgcgaacc gcgcaggcgc tcggcatcga gcgcaccaac
     1621 ctgcacaaga agctgcgcgc gctcggcatc caccgcggcg agggcagcgc cggcggcgac
     1681 catgactagc ccggacggcg gctgacgtgc gcggtccggc ggtcctcgcg gtcctcgcgc
     1741 tccgggccgc gtccgtcgcg agcgc
//
LOCUS       DASVOR010001966         3192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_109342,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001966 DASVOR010000000
VERSION     DASVOR010001966.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109342"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VF516_36525"
     CDS             <1..1201
                     /locus_tag="VF516_36525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113311.1"
                     /translation="DQRQAETLLIDAAHAAMAQGGPRAAVPLQRGLARILLASGDRPA
                     AIEAYRGILNVEPDGASDRVALAEIYAIDDPQRAVGELRKVLDRDIHHAPAYRLLASY
                     YSRTGATERANRVLTVLDLLGFAEDADRATMMRLNATRVRPPLRGVLDDDARVRLLIT
                     QAAREPLGELFDALAQELTSVIVPPSLGHDLQPATAAADPRLVEIATEIGALYQTEAE
                     LFTGEKVPGLAAVTAFPRRMLVVDRSLLTESDLTLRFLCGYAFEAIRGGYATLLQLGA
                     RQRRELGHLLRALLTPDPGVTGTAADLLGSVSPAAQKVIERHSRIRDIDPGAWIDGML
                     ACAKRAGLIACDDFHAAIWTVALLSGEKLASHHATVALGSVLGGPDLVRYYLSDNYQQ
                     IRNLLTS"
     gene            1412..2518
                     /locus_tag="VF516_36530"
     CDS             1412..2518
                     /locus_tag="VF516_36530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113312.1"
                     /translation="MSYVKLGGVIDEDNELGDLVDKIPSGTAVIDLGEIERINSCGVR
                     DWVNWLSKLEYNGTRSVLVECSPAIVAQINLVNNFTGNGVVKSFYVPYFCPECDEEKV
                     LLVEASDMGPPPHEPPTCRCDECDLVMDFDDMPDSYFAFLSNQRKLAEPDKINGAMRD
                     LGRGMEADHSRSGSKVRSRSSQPNLSSGSKPSVPSLPSIQRTSSPVPAPVQASISGSA
                     AGPAGQRGFVHPSRPQLGSKPVPGPGSRPNPMPMGYPAPIGQAAHMTHPSPQMGAPQL
                     GQLGPPTGHPPMPLGHPGQAMPGQVNALGVSPSGPSGHQAGHVNGPGMPGHYSFGAQM
                     RPPAAPGRSNALVVVLIVILVAAIGVLAYLVSTK"
     gene            2832..2981
                     /locus_tag="VF516_36535"
     CDS             2832..2981
                     /locus_tag="VF516_36535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113313.1"
                     /translation="MKQKKVKSKSPRAKVLVVIERLEKVVGAVGAKDAAITTCTDHAY
                     CAPSD"
     gene            3011..>3192
                     /locus_tag="VF516_36540"
     CDS             3011..>3192
                     /locus_tag="VF516_36540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113314.1"
                     /translation="MLTFPWSTPDRSPPLPDHTRRLVEAVRAGRGGGLFPPVIRICRE
                     GVETDARELAGPRDAGL"
BASE COUNT          487 a         1165 c         1071 g          469 t
ORIGIN      
        1 ggatcagcgc caggccgaga ccttgctgat cgacgccgcg cacgccgcga tggcgcaggg
       61 cgggccgcgc gccgccgtgc cgctgcagcg cggcctggcg cggatcctct tggcctcggg
      121 cgaccgcccg gccgcgatcg aggcctaccg cgggatcctc aacgtcgaac ccgacggcgc
      181 cagcgaccgg gtcgcgctcg ccgagatcta cgcgatcgac gacccgcagc gcgcggtcgg
      241 cgagctgcgc aaggtgctcg accgcgacat ccaccacgcc ccggcgtacc gcctgctcgc
      301 gtcgtactac agccgcaccg gcgcgaccga gcgcgccaac cgcgtgctga ccgtgctcga
      361 cctgctcggg ttcgccgagg acgccgaccg cgcgaccatg atgcgactca acgcgacccg
      421 ggtccgcccg ccgctgcgcg gggtgctcga cgacgacgcc cgggtccgcc tgctgatcac
      481 ccaggccgcg cgcgagccgc tcggcgagct gttcgacgcg ctcgcccagg agctgacgtc
      541 ggtgatcgtc ccgccgtcgc tcggccacga cctccagccg gcgaccgccg ccgccgaccc
      601 gcggctggtc gagatcgcga ccgagatcgg cgcgctgtac cagaccgagg ccgagctgtt
      661 caccggcgag aaggtcccgg gtctggccgc cgtgaccgcg ttcccgcgcc ggatgctcgt
      721 ggtcgaccgc tcgctgctca ccgagtccga cctgaccctg cggttcctgt gcgggtacgc
      781 gttcgaggcg atccgcggcg gttacgcgac cctgcttcag ctcggcgcac gccagcgccg
      841 cgagctcggc cacctgctgc gcgccctctt gacccccgac ccgggagtca ccggcaccgc
      901 cgccgacctg ctcggcagcg tcagcccagc cgcccagaag gtcatcgagc gccattctcg
      961 gatccgcgac atcgaccccg gcgcctggat cgacggcatg ttggcatgcg ccaagcgcgc
     1021 gggcctgatc gcctgcgacg acttccacgc ggcgatctgg accgtcgccc tgctgtccgg
     1081 cgagaagctg gccagccacc acgcgaccgt cgcgcttggc tccgtgctcg gggggcccga
     1141 tctggtacgg tactacctga gcgataacta tcagcagatc cggaacttgt tgaccagctg
     1201 agctaccgac ggccggcaaa atgcaggctg gattacatgt acggtgtagg aagatgaccc
     1261 gggcgttcgc cgcgctggag gatttaagtg tcagatcgta cagtggaccg tgcggcagct
     1321 ggaatggctg tggtaatcaa ccataccgtc gttacatgcc ggaggtgcga tgagccataa
     1381 gttccaggca tcggtccagc accgtggcga catgagctac gtgaagctgg ggggcgtgat
     1441 cgacgaggac aacgagctcg gtgacctggt tgacaagatc ccctctggca ccgccgtgat
     1501 cgacctcggt gagatcgagc ggatcaactc gtgcggggtg cgcgactggg tcaactggct
     1561 gtccaagctc gagtacaacg gcacccggtc ggtgctggtc gagtgctcgc cggcgatcgt
     1621 cgcgcagatc aacctggtca acaacttcac cggcaacggc gtcgtcaaga gcttctacgt
     1681 cccctacttc tgcccggagt gcgacgagga gaaggtgctg ctggtcgagg cctccgacat
     1741 gggtccgccg ccgcacgagc cgccgacctg ccggtgcgac gagtgcgacc tggtcatgga
     1801 cttcgacgac atgccggact cgtacttcgc gttcctgtcc aaccagcgca agctcgccga
     1861 accagacaag atcaacggcg cgatgcgcga cctcggtcgc ggcatggagg ccgaccacag
     1921 ccggagcggc agcaaggtcc gctcgcggtc cagccagccc aacctgtcga gcggctccaa
     1981 gccgagcgtg ccgtcgctgc cgtcgatcca gcgcaccagc tcgccggtgc ccgcgccggt
     2041 ccaggcgtcg atctcgggct cggccgccgg ccccgccggc cagcgtggct tcgtccaccc
     2101 gagccggccg cagctcggct cgaagccggt ccccgggccc ggcagccggc ccaacccgat
     2161 gccgatgggg tacccggccc cgatcggcca ggccgcgcac atgacccatc ccagcccgca
     2221 gatgggggcc ccacagctcg ggcagctcgg gcccccgacg ggccatccgc cgatgccgct
     2281 cggccatccc ggccaggcca tgccggggca ggtcaacgcg ctcggggtct caccttccgg
     2341 gccgtccggg caccaggccg gccacgtcaa tggcccaggc atgccgggcc actacagctt
     2401 tggcgcccag atgcgcccgc ccgcagcccc gggccggtcg aacgcgctgg tggtcgtgct
     2461 gatcgtcatc ctggtcgccg cgatcggcgt gctcgcctac ctggtgtcga ccaagtagct
     2521 cggctcgacc cgaccggccg acccgcccgt tcgtacaggt cgttccagcg tggtccgctg
     2581 cgacgcgact gcgactgcga ctgtcgcggt acagcgccgc gacgccccgg ccggaccaca
     2641 gcgcaactgc gcgacattcg tcgattggca atcattggca ttgcggtctt gacttcacac
     2701 cggactgccg ctctgcgcga acggtattgt cacgcagccg atcccggcgc tgcaaaagta
     2761 tcggtggccc atggtgaggt cgtaatccat attcgtagcg agcgctcgct gaggtgctcg
     2821 aggaggacgt gatgaagcaa aagaaggtga agtccaagtc gccacgcgcg aaggtcttgg
     2881 tggtgatcga gcgcctggag aaggtcgtcg gcgcggtcgg cgcgaaggac gcggcgatca
     2941 cgacctgcac cgatcacgcg tactgtgccc cgtcggacta gccggtcggg ctcgaggtga
     3001 ccgcccgtcc gtgctgacgt tcccctggtc cacgcccgac cgctcgccgc cgctgccgga
     3061 ccacacgcgg cggctggtcg aggccgtccg cgccggacgg ggcggcggtc tatttcctcc
     3121 tgtgatccgg atctgccgcg agggcgtcga gacggacgcg cgcgagctcg ccggcccgcg
     3181 cgacgccggc ct
//
LOCUS       DASVOR010001967         3895 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488496,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001967 DASVOR010000000
VERSION     DASVOR010001967.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3895
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488496"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(583..879)
                     /locus_tag="VF516_36545"
     CDS             complement(583..879)
                     /locus_tag="VF516_36545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113315.1"
                     /translation="MDTIAQVACYAADELRYPGDAAARRAFVRMQILVGQAADEASTA
                     LAQGDRLMAALTKHLQAQRDNQDPGDQDDARTRPVRARPTRSSPRARARGARRL"
     gene            complement(977..1810)
                     /gene="istB"
                     /locus_tag="VF516_36550"
     CDS             complement(977..1810)
                     /gene="istB"
                     /locus_tag="VF516_36550"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX8113316.1"
                     /translation="MAALVMLLRALKLPTIAQHAEEIAQLAEREGWTFVRYLHHLVEL
                     EVHERRRRRIQRNLRSAGLPGDKTLATLNRSRLPIKVAKMLPTLCEGAFVERGDNLLA
                     FGRPGRGKTHLVCAIGYELIQRGYRVLFLATYALVQRLLVAKRDLRLEDELAVLDGFD
                     AVICDDLGYVQQNREEMEVLFTFLAERYERRTVIITSNLVFSEWDRIFKDPMTTAAAI
                     DRLVHHAIVIEMTGKSIRIEQAQAELTAEAESPSTPNASPTEPGSAPARTTNEPGSDD
                     V"
     gene            complement(1825..3300)
                     /gene="istA"
                     /locus_tag="VF516_36555"
     CDS             complement(1825..3300)
                     /gene="istA"
                     /locus_tag="VF516_36555"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX8113317.1"
                     /translation="MEEMSKHGQIGQAAMKADMDRKTARKYVAAGKLPSELKQPRDWR
                     TRPDPFEEHWPEVEARLRETPELEAKTLFELLQQQYPGRYEDGQLRTLQRHVKRWRAA
                     QGPDREVVLAQQHRPGEAAQTDFTHATELAVTIAGQLFVHLLCVLVLPYSNWQWATVC
                     LSESILALRKGVQRALFQLGRVPRYHQTDGSTAATHKIPEGQAEHVEGTQRAFNTEYV
                     ALMRHLGMTPRLIELGEKEQNGDVEASNGAIKRRLEQALLVRGSRDFESVDAWQAFID
                     ETLRKANANRGRRVAEDLAAMRELNVAKLPEYVEEGVKVSEWSTVRVKHCAYSVPSRL
                     MGEWVRVRIFEDRLEVRFADELQLACERLRGRGLHRIDYRHVIWSLVRKPGGFARYVY
                     REEMFPSLVFRAAYDAIQAARPGTKGDLEYLRILHLAASTMEADVAAALAQLQTAGTP
                     ITVDAVKALIAPPAAVAVPELAAMPVDLTEYDALLDAQEAA"
BASE COUNT          526 a         1332 c         1359 g          678 t
ORIGIN      
        1 cttcgtgccc aggccaaggc ggcgctgacc ggcctgggct ggaagcctgc gatcgcgcac
       61 gccgcggtcg ctgccgcggc agaggcgcag ggggacacgc cgacgctgga gcgcatgatc
      121 tttgaagcat tgcggcgatg ccccacgccg aaggtgtgag gtgagtgcct ggcgtcaact
      181 agattctccc atcaacgaca tctacatctc cccatcgatc tcggttcgtt gaatgcggtt
      241 cttggtcttc tgagtttcgt cgtgggatcc tccttggtga gtggctgagc ggttgctggg
      301 gttgtcgggc caggtgccgt cggtgctgtg cccgggatgg ggatcgtggt tctcatggaa
      361 acgaggcggc aggcgacgtt gtgatcggtg acagtgctcc tggggtagtt gtcgtcgggg
      421 tagttgtcgt cggggccgag ccaggagccg tcggtgctgc gctcgggatg gggtggatgg
      481 gcggggtggt gggggcgcat ggtcgtcgcg gaggccgagc caggggccgt cggtgctgcg
      541 ctcgggatgg ggtggatggg cggcacgaga tgggtcgcgg cgtcagagcc gtcgagcacc
      601 gcgagctcgt gctcgaggcg aagatcgcgt cggacgagca cgcacgggac gagtgcgtgc
      661 gtcgtcctga tcgccagggt cctggttgtc gcgttgggcc tgcaggtgtt tcgtgagcgc
      721 tgccatgagc ctgtcacctt gcgcgagcgc cgtgctcgcc tcgtcggccg cttgcccgac
      781 gaggatctgc atccgcacaa aggcgcgtcg cgctgcggcg tcgccgggat agcgcagctc
      841 atccgccgca tagcaggcga cctgtgcgat ggtgtccacg cgcaagatcg cagcacggag
      901 gctctcgatt gcattcgcag ggcgaaagtc gtctgacgga gcctggcgcg cgatcgtgcc
      961 gtgaagatcg cgagggtcag acatcgtcgc tcccaggctc gttcgtggtc ctcgcgggtg
     1021 ccgagccagg ctccgtcggg cttgcgttcg gcgtagacgg gctttcggct tcggcagtga
     1081 gctcggcctg tgcttgctcg atccggatgc tcttgccggt catctcgatg acgatcgcgt
     1141 ggtgaacgag ccggtcgatc gcggcggcgg tggtcatcgg gtccttgaag atccgatccc
     1201 actcgctgaa caccaggttg ctggtgatga tcacggtgcg ccgctcgtag cgctcggcga
     1261 ggaatgtgaa cagcacctcc atctcctcgc ggttctgctg gacgtatcca agatcgtcgc
     1321 aaatcacggc gtcgaagccg tcgagcaccg cgagctcgtc ctcgagacgc agatcgcgct
     1381 tggcgacgag caggcgctgg acgagggcgt aggtcgcgag gaacagcacg cgatacccgc
     1441 gctggatcag ctcgtagccg atcgcgcaca ccaggtgcgt cttgccgcga cccggccggc
     1501 cgaacgcgag caggttgtca ccacgctcga cgaacgcgcc ctcgcacagc gtcggcagca
     1561 tcttggcgac cttgatcggc aagcgcgaac ggttgagcgt cgcgagcgtc ttgtcgcctg
     1621 gcagccccgc gctgcgcaga ttgcgctgga tccggcgccg gcggcgctca tgcacctcga
     1681 gttcgacgag gtggtggagg tagcggacga acgtccagcc ttcgcgctcg gcgagctgcg
     1741 cgatctcctc ggcgtgctgc gcgatggtgg gcagcttcag cgcgcgcagc aacatcacga
     1801 gcgcggccaa cggtgcggac gccatcatgc ggcctcctgg gcgtcgagca gtgcgtcgta
     1861 ctcggtgaga tcgaccggca tcgctgcgag ctcggggacc gcgaccgccg ccggcggcgc
     1921 gatcagcgcc ttgaccgcgt cgacggtgat cggcgtgccg gcggtctgca gttgcgcgag
     1981 cgccgccgcg acgtcggcct ccatcgtgct cgccgcgagg tgcaggatcc tgaggtactc
     2041 gaggtcgccc ttggtccccg gccgcgcggc ctggatcgcg tcgtaggccg cgcggaacac
     2101 cagcgacggg aacatctcct cgcggtacac gtagcgcgcg aacccgcccg gcttgcggac
     2161 gagcgaccag atcacgtgtc ggtagtcgat ccgatgcagg ccgcgaccgc ggagccgctc
     2221 gcacgcgagc tggagctcgt cggcgaacct gacctcgagc cggtcctcga agatccggac
     2281 gcggacccac tcgcccatca gccgcgatgg caccgagtac gcgcagtgct tgacgcgcac
     2341 cgtgctccac tcgctgacct tcacgccctc ctcgacgtac tcaggcagct tcgcgacgtt
     2401 gagctcgcgc atcgccgcga ggtcctcggc gacgcggcgc ccgcgattcg cgttggcctt
     2461 gcgcagcgtc tcgtcgatga acgcctgcca tgcgtccacg ctctcgaagt cgcggctgcc
     2521 gcgcaccagt agcgcctgct cgagccggcg cttgatcgcg ccgttgctgg cctcgacgtc
     2581 gccgttctgt tccttctcgc cgagctcgat gagccgcggc gtcatcccga ggtgccgcat
     2641 cagcgcgacg tactcggtgt tgaacgcccg ctgggtgccc tcgacgtgct ccgcctggcc
     2701 ctccgggatc ttgtgcgtcg ccgccgtcga gccgtcggtc tggtggtacc gcggcacccg
     2761 cccgagctgg aacagcgctc gctgcacgcc cttgcgcagc gccaggatcg actccgacag
     2821 gcagaccgtc gcccactgcc agttcgagta cggcagcacg agcacgcaga gcaggtgcac
     2881 gaacagctga cccgcgatcg tcaccgcgag ttcggtcgcg tgcgtgaagt cggtctgtgc
     2941 cgcctcgccg ggccggtgct gctgtgccag caccacctcg cgatccggcc cctgggccgc
     3001 gcgccaccgc ttcacgtggc gctgcagcgt gcggagctgg ccgtcctcgt agcggccggg
     3061 gtactgctgc tgcagcagct cgaacagcgt cttggcctcg agctccggcg tctcccgcag
     3121 cctggcctcg acctccggcc agtgctcctc gaacggatcc gggcgcgtcc gccagtcgcg
     3181 cggctgcttc agctccgacg gcagcttgcc ggcggcgacg tactttcgcg ccgtcttccg
     3241 gtccatgtcc gccttcatcg cagcctggcc tatctgtccg tgtttgctca tctcttccat
     3301 cagcttcctc acttgagcgt cgctcactgg tcgcacgggt gcctgggcct ccacctccgg
     3361 gagaccacgg ccctctgacc ggtcagctac gccgtcgcgg atggggcgat gtaattgtcg
     3421 ccgctgggga gatctagttg tcgctcagca gcaagacgca gcctcggctc gcggtcgccg
     3481 cgccggtgta ctggtcttga gtcgcggagc gcggtccgcg cgccccgact ccgcaggacg
     3541 cgccgagcgc gcgtcgccag ccaatagctc ggcgcgactt gacgcatcgt gcgccatgcc
     3601 ccgccggagc tcaccacgcc cgcgtcgccg agctggtcga ggatgatgcc gccctcggcg
     3661 agccgcccga gttcctgagc gacgtcgccg gcgagcaccg agcggcgtgc tccgacccgg
     3721 gccgggactg tcttgcggct tgcggcggcg gatccgatgt cgctgtcgta gctcgctggc
     3781 aacgcggcag ccgcagccca gcaccgtcac ggcgcccgtc tcgcgaccgc cgccggcacc
     3841 tcagcgaccg gccgccgctc cagcgcctcg cggatcatcg cgccgtgccg catct
//
LOCUS       DASVOR010001968         2378 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_371505,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001968 DASVOR010000000
VERSION     DASVOR010001968.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371505"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VF516_36560"
     CDS             complement(<1..503)
                     /locus_tag="VF516_36560"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEX8113318.1"
                     /translation="MLDALRTRLSRAALYARAVRSTGSLELARPAQLVEFLRGATKVP
                     LGPHLAVMYQAAAHPDREALIEYAEHGVRRMTWGELAATTNRLANALHARGAIGGSRV
                     AVMLPNGGEYLIAQHALGLLGATAVQIGYRLKAGEIAHILSNAEPDATLVHTDYLAAM
                     TEARAQAR"
     gene            complement(575..>2378)
                     /locus_tag="VF516_36565"
     CDS             complement(575..>2378)
                     /locus_tag="VF516_36565"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113319.1"
                     /translation="GAQVGLAAAQPPAAAQRRETDLLAILQRKDIAAADPRAVARAWM
                     LAGNAAMRAGRYDVARERLRKARDLNPQDLAATGELAETELRDGKIGTAAGLTSQVLS
                     TSQDNVAAQLVQSEIEIKQRKFSIAGQRLDALAARTPPLAPLELARLLLVRGKLADAQ
                     GDGQAAVDAFIKGAQAARDLDLEPMVAAVGKLAALARAAAAEHDTPRADKLRAEIEEL
                     LGNFADQAARDPQLALTLGISYLQAGNAEKAEPWLRRAVEGLAKDAEARFQLGRALLL
                     LDRPQDALEALRAALALDPARLDITAELARTYEALQRDHDADQLYAKLIAGPDPGLEL
                     RARAGRFYARTGAIDKAAAQGEKILATDPRDAAGHYLKGEGLLAAGKLPEAKQQFQRA
                     VEADRDPQYLDALGRAAEAIGQGGDRAAQELALRSYAEAAAASTLINPRVGQGRIYLL
                     RHEPASAVPHLSDAVRLSPPNAETLFLLGAAYQDSQHPAEARHWLGEAVKIAAIPEAY
                     WRIAQLEHEANHGPAAILMLTDAIRLAGEAETKTGKTVPWLTDALYLQGRVNFDLHNE
                     AAARAAWQLYLARKPPASAQRSEVEQLLATRLRP"
BASE COUNT          252 a          823 c          964 g          339 t
ORIGIN      
        1 cgcgcctggg cgcgggcctc ggtcatcgcg gcgaggtagt cggtgtggac cagcgtcgcg
       61 tcgggctcgg cgttgctcag gatgtgggcg atctcgccgg ccttgagccg gtagccgatc
      121 tggaccgcgg tggcgccgag caggccgagc gcgtgctggg cgatcaggta ctcgccgccg
      181 ttgggcagca tcaccgcgac ccggctgccg ccgatggcgc cgcgcgcgtg cagcgcgttg
      241 gcgagccggt tggtggtcgc cgcgagctcg ccccaggtca tccgccggac gccgtgctcg
      301 gcgtactcga tcagcgcttc gcggtcgggg tgcgccgcgg cctggtacat caccgcgagg
      361 tgcgggccga gcgggacctt ggtggcgccg cgcaggaact cgacgagctg cgccggccgg
      421 gccagctcga gcgagcccgt ggagcgaacg gcgcgcgcgt agagcgccgc gcgggacaac
      481 ctcgtgcgaa gggcgtcgag cacgtcccac catactgcgg ctgcgcccga aagttcacct
      541 caccggcgcg cccgatggcc cggtgagttt cggatcacgg ccggagcctg gtcgcgagca
      601 gctgctcgac ctcggagcgc tgggccgagg ccggcggctt gcgcgcgagg tagagctgcc
      661 aggccgcgcg cgcggcggcc tcgttgtgga ggtcgaagtt gacccggccc tggaggtaga
      721 gcgcgtcggt cagccacggc accgtcttgc cggtcttggt ctcggcctcg ccggcgagcc
      781 ggatcgcgtc ggtcagcatg aggatcgccg ccggcccgtg gttggcctcg tgctcgagct
      841 gggcgatccg ccagtaggcc tcggggatcg ccgcgatctt cacggcctcg ccgagccagt
      901 ggcgggcctc ggccgggtgc tggctgtcct ggtaggccgc gccgagcagg aacagggtct
      961 cggcgttggg cggagacagc cgtacggcgt cgctcaggtg cggcaccgcg ctggccggct
     1021 cgtgccggag caggtagatc cggccctggc cgacccgcgg gttgatcagg gtggacgcgg
     1081 ccgcggcctc ggcgtagctc cgcagcgcga gctcctgggc cgcgcggtcg ccgccctgcc
     1141 cgatcgcctc ggcggcgcgg ccgagcgcgt cgaggtactg gggatcgcgg tcggcctcga
     1201 ccgcgcgctg gaactgctgc ttggcctcgg gcagcttgcc cgcggcgagc agcccctcgc
     1261 ccttgaggta gtgcccggcg gcatcgcgcg gatcggtcgc gaggatcttc tcgccctgcg
     1321 cggcggcctt gtcgatcgcg ccggtgcggg cgtagaaccg gccggcgcgg gcgcgtagct
     1381 cgaggccggg gtcggggccg gcgatcagct tggcgtacag ctggtccgcg tcgtgatcgc
     1441 gctgcagcgc ctcgtaggta cgggcgagct cggcggtgat gtcgagccgc gccggatcga
     1501 gggcgagggc ggcgcgcagc gcctcgagcg cgtcctgggg ccggtcgagc agcagcagcg
     1561 cgcggccgag ctggaaccgg gcctcggcgt ccttggcgag cccctcgacg gcgcggcgca
     1621 gccacggttc ggccttctcg gcgttgccgg cctggaggta gctgatgccg agggtcagcg
     1681 cgagctgcgg atcgcgcgcg gcctggtcgg cgaagttgcc gagcagctcc tcgatctcgg
     1741 cgcgcagctt gtcggcgcgc ggggtgtcgt gctcggcggc cgcggcgcgg gccagcgcgg
     1801 cgagctttcc gaccgcggcg accatgggtt cgaggtcgag atcgcgcgcg gcctgggccc
     1861 ccttgatgaa ggcgtcgacg gcggcctgac cgtcgccctg ggcgtcggcg agcttgccgc
     1921 ggaccagcag cagccgggcg agctcgagcg gggccagcgg cggggtgcgg gcggcgagcg
     1981 cgtcgagccg ctgcccggcg atcgagaact tgcgctgctt gatctcgatc tcgctctgca
     2041 cgagctgggc ggcgacgttg tcctgcgacg tcgagagcac ctgcgaggtc aggcccgccg
     2101 cggtgccgat cttgccgtcg cgcagctcgg tctcggcgag ctcgccggtc gcggcgaggt
     2161 cctgcgggtt gaggtcgcgc gccttgcgga gccgctcgcg cgcgacgtca tagcgcccgg
     2221 cgcgcatcgc cgcgttcccg gcgagcatcc acgcccgcgc caccgcgcgc ggatcggcgg
     2281 ccgcgatgtc cttgcgctgg aggatcgcga gcaggtcagt ctcgcggcgc tgcgcggcgg
     2341 cgggcggctg cgcggcggcg agcccgacct gggcgccg
//
LOCUS       DASVOR010001969         5942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_21913,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001969 DASVOR010000000
VERSION     DASVOR010001969.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21913"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5942)
                     /locus_tag="VF516_36570"
     CDS             complement(<1..>5942)
                     /locus_tag="VF516_36570"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8113320.1"
                     /translation="YTGTGTSLSILAGRLAYLLDLQGPSVALDTACSSSLVAVHLACQ
                     SLRTGDCRLALAGGVNLILSPSTTILASQMRMLAADGRCKAFDAHADGFVRGEGSGVV
                     VLKRLSDALADGDAIRALIRGSAVNGDGRTNGLTAPNGRSQQAVIEQALKRASVEPGE
                     ISYVEAHGTGTSLGDPIEMEALAAAIGQAQPGRSTCAVGSVKTNVGHLEAAAGVAGLI
                     KVVLSLEHEAVPPILHFQTLNPNISLDGTRLAIPTELTPWQRNGKRRVAGASSFGWSG
                     TNAHVVVEEAPAVTAMAEAEPARALVLPISAQSPEALRALAGAYHDFLSRADAPAVRD
                     LCYSAAVRRTHHDHRLAVVGESHAELAEALSAFLAGQPHHRLTVGAHAVSADHKLVFV
                     FTGQGGQWPGMAQDLLAREPVFAEAIAACDAALKPHVDWSLGELLSVDPTSPLLDRID
                     FVQPVLFSFQVAVAALWRSLGIVAQAVVGHSMGEVAAAYVAGALSLDDAACVIAHRSR
                     LMRTISGRGAMAVVELPLADTERALAGREDRLALAASNGPTTTVVSGDPAALAALLEE
                     LGRREVFCRPVKVDVASHSPQVDALRPQLLAALAKLSPRTAAIPFHSTVTGAPADGRE
                     LDAAYWSKNLRQPVLFAPVVEHLIAEGHDLFVELSPHPVLGGSVQQLLRGHGDGAVFA
                     SLRRDEPGRASMLAALGGLYAHGYPVDWSRLYPSPGRVVSLPSYPWQRERFWHAPKAS
                     APAAVADRSDPTAHPLLGAAVTLAHAPGHRIWQSVVDLKRLSYVDDHRVQGLAVLPGA
                     GYVEMAMAAAAEVFGDQERSLSSFEFQRLLFFPEDTARVVQTSLVADDAGGSTFQVHS
                     RPADSTDAPWTLHATAWINATSDALASTSGAEILAGARQRCRENTATAEFYREFAERG
                     NQWGPRFQGIAALWRGQREALAELRVPDGLESELARYRIHPAVLDACLQVLGATVPVE
                     ATGTTQSFVPVHVERVVVHRRPHGVRLWSHAQLDADTTPGEASFAGNVRLLDEDGTVI
                     VELLGLRGRGLDRDAIAVPAQLDWFHELRWPSKALAASSTPAAPGRWLIFSDQGGVGA
                     ALAARLRAGGGEAVCVRAGTSFERHPDGSFRVRPDAADDLERLIAAMTPTAALPWRGV
                     IHLWSLDTAEPDTAAQLTDAQVPTCSFATRLLQALSALPAAPRLWLVTQGAQAVTPGD
                     GPTALVQAPLWGLGRTIPLEHPALWGALVDVDPTAAVEASAAQLLNEVSHPDGEDQIG
                     YRNSTRHVARLAASTMSKDPAQIELRPDGAYLVTGGLGGLGLAAAERLVERGARHLVL
                     LGRSGLPDRAGWRDVPGDTPLASTIAAIERLESHGATVHVASVDVADEDRLAASLAAL
                     HAAGLPPIHGVIHAAGVAELVPLQELDPATLLSVLRPKVQGGFALHRVLAHEPLDFFV
                     LYSSMASILSSPLLGAYSAANAFLDALAQHRRALGQPAISVGWGYWEQIGMGVRFQAA
                     RGREGTAEGMGSFSPAQGLAALERVLVADPVHIGVMPIDWNRWTAAHAEAARLPVLAD
                     VRGTPASAAPGRRAPKVDVDALLAAPAEDRAQALASYLRRALARVLRLPEARIDIAHP
                     LNRLGLDSLMAVELKNTIEADLGVMVPVVRFLQGPSIEQLAAQLAELLASRVARAGTE
                     AAAPDPGLPMIVPAPGARCEPFPLNDIQQAYWVGRNGFFELGSVAAHMYVEVDGTDLD
                     IDRLQDAWQHLVARHDMLRAVILPDGRQQVLPAVPAYVIGVDDLRGMDPDRAAGRLEA
                     TREQLSHQVMPTDRWPLFEIRASRLDDHRVRIHLSLDILIADVWSWQTLFREWAALYG
                     NPSTALPPLEITFRDYVLAERKLESSEAQRRSLAYWRDRIPTLPPAPELPLSSTADAA
                     AQGRFVRRTATLDAQAWRRLKARAHEAGLTPSGVLLAAFSEVLATWSKSSRFTLNVTL
                     FNRLPLHPQVNDIVGDFTSVNL"
BASE COUNT          882 a         2081 c         2215 g          764 t
ORIGIN      
        1 caggttcacg gacgtgaagt cgccgacgat gtcattgacc tgcggatgca gcggcaggcg
       61 attgaacagc gtgacgttga gcgtgaaccg ggagctcttg ctccaggtcg ccaggacctc
      121 ggagaacgcc gccagcagca ctcccgaggg ggtgagcccg gcttcgtgtg cgcgcgcctt
      181 gagccgccgc catgcctggg cgtcgagcgt cgcggtccgg cggacgaacc ggccctgcgc
      241 cgccgcatcc gccgtcgacg acagcggcag ctccggggcc gggggcaagg tcgggatccg
      301 atcgcgccag tacgcgaggg atcgccgctg cgcctcgctg ctctcgagct tgcgctcggc
      361 gaggacgtag tcgcggaacg tgatctcgag cggcgggagc gcggtggacg ggttgccgta
      421 cagcgcggcc cactcgcgga acagggtctg ccagctccag acgtcggcga tcaggatgtc
      481 gagcgacagg tgaatcctga cgcggtggtc gtcgagccgc gaggcccgaa tctcgaacag
      541 cggccatcga tccgtgggca tcacctggtg cgacagctgc tcgcgcgtgg cctcgagcct
      601 tccggccgcg cggtcgggat ccatgccgcg caggtcatcg accccgatga cataggccgg
      661 caccgccggc aggacctgct gtcgcccgtc cggcaagatc accgcgcgca gcatgtcgtg
      721 gcgggccacc agatgctgcc aggcgtcctg cagccggtcg atgtcgagat cggtgccgtc
      781 gacctcgacg tacatgtgag cggccaccga gccgagctcg aagaacccgt tgcggccaac
      841 ccagtaagct tgctggatgt cgttgagcgg gaacggctcg cagcgagcac ccggcgccgg
      901 cacgatcatc gggagcccag gatccggggc cgcagcctcg gtgcccgcgc gcgcgacccg
      961 gctggcgagg agttcggcga gctgcgccgc gagctgctcg atgctcgggc cttgcaggaa
     1021 ccgcacgacc ggcaccatca cgccgaggtc cgcctcgatg gtgttcttga gctcgacggc
     1081 catgagcgag tcgaggccga gccggttcaa ggggtgcgcg atgtcgatgc gagcctcggg
     1141 cagccggagc acgcgcgcaa gcgcgcgacg caggtaggac gccagggcct gcgcccgatc
     1201 ttcggccggc gccgcgagca gcgcatcgac gtcgactttc ggagctcggc gccccggtgc
     1261 cgcgctcgcc ggtgtgccgc ggacgtcggc gagcactggc agccgggccg cctcggcgtg
     1321 ggccgctgtc cagcggttcc agtcgatcgg catgaccccg atgtgcaccg ggtccgcgac
     1381 gagcacgcgc tcgagcgcgg ccaggccctg cgcgggcgag aagctgccca tcccctcggc
     1441 ggtgccttcg cgccctctgg ctgcctggaa ccgcacgccc atgccgatct gctcccagta
     1501 gccccagccg acgctgatgg cgggctggcc gagcgcgcgg cggtgctggg cgagcgcgtc
     1561 gaggaaggcg ttcgcggccg agtacgcacc gagcaggggc gagctcagga tcgacgccat
     1621 cgatgagtac aggacgaaga agtcgagcgg ctcgtgcgcg agcacgcgat ggagcgcgaa
     1681 cccgccttgc accttgggtc gcaggaccga gagcagcgtt gccggatcga gctcctgcag
     1741 tggcacgagc tcggcgacac cggcggcgtg gatcacgccg tggatcggcg gcaaccctgc
     1801 cgcatgaagc gcggcgaggg atgcggccag gcggtcctcg tccgcgacgt cgacggaggc
     1861 gacgtgcacg gtcgcgccgt gggactcgag tcgctcgatc gcggcgatcg tgctggccag
     1921 cggtgtgtcg ccgggaacgt cgcgccagcc ggcgcgatcg ggcagaccgc tgcggccgag
     1981 gaggacgagg tggcgcgcgc cgcgctccac cagccgctct gccgcggcga gcccgaggcc
     2041 gcccaggccg ccggtcacga ggtaggcgcc gtcgggtcgg agctcgatct gcgccgggtc
     2101 cttggacatc gtgctcgccg cgagccgcgc gacgtggcgc gtgctgttcc ggtagccgat
     2161 ctggtcttct ccgtcaggat ggctgacctc gttcaggagc tgtgcggcgc tggcctcgac
     2221 ggcggcggtg ggatcgacgt cgacgagcgc gccccacagc gccgggtgct cgagcgggat
     2281 ggtccggccc aggccccaga gcggcgcttg caccagcgcc gtcgggccat cgcccggagt
     2341 gaccgcctgt gcaccctgcg tgacgagcca cagccgcgga gccgccggca gcgcggacag
     2401 cgcctgcagg agccgcgtcg cgaagctgca ggtgggcacc tgcgcgtcgg tcagctgcgc
     2461 cgccgtgtcg ggttccgcgg tatcgaggct ccacagatgg atcaccccgc gccacggcag
     2521 cgcagccgtc ggggtcatgg ccgcgatcag gcgctcgagg tcgtcggccg cgtcggggcg
     2581 aacgcggaag gagccgtccg ggtggcgctc gaagctcgtc cccgcgcgca cgcagacagc
     2641 ttcgccaccg cccgcgcgca ggcgcgccgc cagcgcggct ccgacgcccc cttggtcgga
     2701 gaagatcagc cagcgtccgg gcgccgcagg cgtggagctc gccgccaggg ccttcgatgg
     2761 ccagcgcagc tcgtggaacc agtcgagctg cgcgggcacg gcgatcgcgt cgcgatcgag
     2821 cccgcgaccg cggagcccga ggagctcgac gatcacggtc ccatcctcgt cgagcagacg
     2881 cacgttacct gcgaacgagg cctcgcccgg ggtggtgtcc gcatcgagct gggcatggct
     2941 ccacaaccgc acgccgtgcg gccggcgatg gaccaccacg cgctcgacat ggaccggcac
     3001 gaacgactgg gtcgtgccgg tggcctcgac cgggacggtg gcaccgagaa cctggagaca
     3061 ggcatcgagc accgccggat ggatccggta ccgcgcgagc tcggattcga gcccgtccgg
     3121 gacgcgcagc tcggcgagcg cctcgcgctg gccgcgccac agcgcggcga tgccttgaaa
     3181 ccgcggaccc cactggtttc cacgctcggc gaactcccgg tagaactcgg cggtggcggt
     3241 gttctcgcgg cagcgctgcc gggcgccggc gaggatctcc gcgccgctcg tcgatgcaag
     3301 cgcgtcggag gtcgcattga tccaagcggt ggcgtgcaag gtccacggcg cgtcggtgga
     3361 gtccgccgga cggctgtgga cctggaacgt cgatccaccc gcgtcatcgg cgaccaggct
     3421 ggtctgcacg acccgcgcgg tgtcctcggg gaagaacagc agacgctgga actcgaacga
     3481 cgacagcgag cgttcctggt ccccgaagac ctccgcggcc gcggccatcg ccatctcgac
     3541 gtacccggcg ccaggcaaca cggccagtcc ctggacccgg tggtcatcga cgtagctcag
     3601 gcgcttcaga tccaccaccg actgccagat ccggtgaccc ggcgcgtggg ccagcgtcac
     3661 cgcggcaccg agcagcgggt gcgcggtcgg atcgctccgg tcggctacag cggcgggcgc
     3721 ggaagccttc ggcgcatgcc agaaccgctc tcgttgccag gggtaggacg gcagcgagac
     3781 cacgcggccc ggcgagggat acagccgcga ccagtcgacc gggtagccgt gggcgtacag
     3841 cccaccgagc gccgcgagca tgctggcgcg gccgggttcg tcacggcgca gcgaggcgaa
     3901 caccgcgccg tcgccgtgac cgcgcagcag ctgctgcacc gagccgccga gcaccgggtg
     3961 cggcgacagc tcgacgaaca gatcgtggcc ttcggcgatc aggtgctcga ccacgggtgc
     4021 gaacagcacc ggctggcgga ggttcttgct ccagtacgcg gcatcgagct cgcggccgtc
     4081 agccggcgcg cccgtgacgg tggagtggaa cgggatggcg gcggttctcg gcgacagctt
     4141 cgcgagcgcg gccaggagct gcgggcgcag cgcgtcgacc tgcgggctgt gcgacgcgac
     4201 atcgaccttg accgggcgac agaacacctc gcgccgaccg agctcctcga gcagggcggc
     4261 gagcgcggca ggatcgccgg agaccacggt cgtggtgggc ccgttgctgg cggcgagcgc
     4321 gagccggtcc tcgcggccgg ccagcgcgcg ctcggtgtcg gccagcggca actcgaccac
     4381 ggccatcgcc ccccggccgc tgatcgtacg catgagacgg ctccggtgag cgatgacgca
     4441 cgccgcatcg tcgagcgaca gcgcaccggc gacgtacgcc gcggcgacct cacccatgct
     4501 gtgcccgacc accgcctggg ccacgatgcc gagcgatcgc cacagcgcgg cgaccgcgac
     4561 ctggaacgag aacagcaccg gctgcacgaa gtcgatgcga tcgaggagcg gcgacgtggg
     4621 gtcgaccgag agcagctcgc ccagcgacca gtcgacgtgg ggctttagcg cggcatcgca
     4681 cgcagcgatc gcctcggcga acaccggctc gcgcgcaagc agatcctggg ccatgccggg
     4741 ccattgacca ccttgaccgg tgaagacgaa caccagcttg tggtccgcgg acacggcatg
     4801 agcaccgacc gtcaggcggt gatgaggctg gcccgcgagg aacgcggaca gcgcctcggc
     4861 gagctcggcg tggctctcgc cgaccacggc gagccggtgg tcatggtggg tgcggcggac
     4921 cgcggcggag tagcagaggt cgcgcacggc cggagcatcg gcgcgagaca ggaagtcgtg
     4981 gtacgcgccg gcgagcgcgc gcagcgcctc cgggctctgc gccgagatcg ggagcacgag
     5041 cgcccgggca ggctccgcct cggccatcgc tgtgaccgcc ggggcctctt ccaccaccac
     5101 gtgcgcgttg gtgccggacc agccgaacga gctcgcaccg gcgacgcgcc gcttgccgtt
     5161 gcgctgccac ggcgtgagct cggtcgggat ggcgagccgc gtcccgtcga gtgagatgtt
     5221 ggggttgagg gtctggaagt gcaggatcgg cgggacggcc tcgtgctcga gcgagagcac
     5281 caccttgatg agcccggcga caccggcggc cgcctcgagg tggccgacgt tggtcttcac
     5341 cgagcccacc gcgcaggtcg accgccccgg ctgggcctgg ccgatcgccg cggcgagcgc
     5401 ttccatctcg atcggatcgc cgagcgacgt cccggtgcca tgcgcctcga catacgagat
     5461 ctcccccggc tcgacgctgg cgcgcttgag tgcctgctcg atgaccgctt gctgcgagcg
     5521 tccattgggc gcggtgagcc cgttggtccg gccgtcgccg ttcacggccg agccgcggat
     5581 cagcgcgcgg atcgcgtcgc cgtccgcgag ggcgtccgag agccgcttca gcacgaccac
     5641 gccgctcccc tcgccgcgga cgaagccgtc ggcgtgcgcg tcgaatgcct tgcagcggcc
     5701 gtccgccgcg agcatccgca tctgggaggc gaggatcgtc gtgctgggcg acaggatgag
     5761 gttcacgccg ccggcgaggg cgagccgaca gtcccccgtg cgcaggctct ggcaggcgag
     5821 gtgcaccgcc acgagcgagg acgagcacgc ggtgtcgagc gccacgctcg ggccctgcag
     5881 atcgaggagg tacgccagcc ggccggcgag gatgctgagc gacgtgccgg tcccggtgta
     5941 gg
//
LOCUS       DASVOR010001970         2128 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_79260,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001970 DASVOR010000000
VERSION     DASVOR010001970.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79260"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..481)
                     /locus_tag="VF516_36575"
     CDS             complement(<1..481)
                     /locus_tag="VF516_36575"
                     /inference="COORDINATES: protein motif:HMM:NF015639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HEX8113321.1"
                     /translation="MDSIYFTWPDRRFAYDCRGCAACCKGHGIGIDVAGGQLVQLLAR
                     RPALAAFLRRRGDAITAFNPRDRCWFLDDGGLCRIEVDDGRAAKPASCRLFPFNRVFR
                     LGGLTIVDYNSVICPLRVAPDGDGAGVSHADVAAEIAGIADPAVVGTPLPARDPDAEG
                     "
     gene            complement(503..1639)
                     /locus_tag="VF516_36580"
     CDS             complement(503..1639)
                     /locus_tag="VF516_36580"
                     /inference="COORDINATES: protein motif:HMM:TIGR03187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein"
                     /protein_id="HEX8113322.1"
                     /translation="MLATQIRQKDSVFYFCAYPADDILDKVRFISRFYAEGEPSLAGE
                     AVDEGDEVAAFISRIEATDAAFQRQLSRAKVTTIKNFYETAASQPPIPGTVLLFTEER
                     LQFDPVKPFTNVGNLTEPDGKYLIIDGQHRLAALHFYRRQHPEDATTIHVPCVIFDGK
                     SEDFATEMFVIINSTPTRINKSHLVDLYEKVSWAAPDKKFAARVVEQLYREPDSPLRY
                     KINRLGGRSKQEKWILQAELFNEIHRWASADWKKLQKKANLAREAERFYEIVRDFLRA
                     AEKAWGDAWGHPNYMVTRPVTLKALLRVCADLAAEDTDAGDRVKRWARRLAPWTELAK
                     QFRVDGFYERFPAKGQVERVARIYRDLARAAGIERRGKADDDEE"
     gene            complement(1657..>2128)
                     /locus_tag="VF516_36585"
     CDS             complement(1657..>2128)
                     /locus_tag="VF516_36585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEX8113323.1"
                     /translation="DDQGAQYDAAGNLASWWQPDTERQFQQRTRCVIDQYSGYEVSGG
                     TRLNGAHTVGENIADIGGVKLALAALHQLRGGAPDTVIAGGFTEDQQFFLGFGQAWCA
                     KARPDYERLLAMVDVHAPPRWRVNGALAATPDFARAFRCKAGSKMVPAKQCVIW"
BASE COUNT          283 a          704 c          797 g          344 t
ORIGIN      
        1 ggccctcggc gtcgggatcg cgcgcgggga gcggggtgcc gacgacggcg gggtcggcga
       61 tgccggcgat ctcggcggcg acgtcggcgt gggacacgcc ggcgccgtca ccgtccggcg
      121 cgacgcgcag cgggcagatc acgctgttgt agtcgacgat ggtgaggccg ccgagccgga
      181 acacgcggtt gaacgggaac agccggcacg acgcgggctt ggcggcgcgg ccgtcgtcga
      241 cctcgatccg gcacaggccg ccgtcgtcga ggaaccagca gcgatcgcgc gggttgaacg
      301 cggtgatcgc gtcgccgcgg cggcgcagga acgccgcgag cgccgggcgg cgggcgagca
      361 gctggacgag ctggccgccg gcgacgtcga tgccgatgcc gtggcccttg cagcacgccg
      421 cgcagccgcg gcagtcgtag gcgaaccgcc ggtcgggcca cgtgaagtag atcgaatcca
      481 cgagttcctc gtcgtttctc gtctactcct cgtcgtcgtc ggccttgccg cggcgctcga
      541 tcccggcggc gcgcgcgagg tcgcggtaga tgcgggcgac gcgctcgacc tggcccttgg
      601 cggggaaccg ctcgtagaag ccgtcgaccc ggaactgctt ggcgagctcg gtccacggcg
      661 cgagccggcg cgcccagcgc ttgacccggt cgcccgcgtc ggtgtcctcg gcggcgaggt
      721 cggcgcacac gcggagcagc gccttgagcg tgaccggccg ggtcaccatg tagttcgggt
      781 ggccccacgc atcgccccac gccttctcgg cggcgcgcag gaagtcgcgc acgatctcgt
      841 agaaccgctc ggcctcgcgc gcgaggttcg ccttcttctg gagcttcttc cagtcggcgc
      901 tggcccagcg gtggatctcg ttgaacagct cggcctggag gatccacttc tcctgcttgc
      961 tgcggccgcc gagccggttg atcttgtagc gcagcgggct gtcgggctcg cggtagagct
     1021 gctcgacgac gcgcgccgcg aacttcttgt cgggggcggc ccacgacacc ttctcgtaga
     1081 ggtcgaccag gtggctcttg ttgatccggg tcggcgtcga gttgatgatc acgaacatct
     1141 cggtcgcgaa gtcctcgctc ttgccatcga agatcacgca cgggacgtgg atcgtggtgg
     1201 cgtcctcggg gtgctggcgg cggtagaagt gcagcgcggc gagccggtgc tggccgtcga
     1261 tgatcaggta cttgccgtcg ggctcggtca ggttgccgac gttggtgaac ggcttgaccg
     1321 ggtcgaactg cagccgctcc tcggtgaaca gcagcacggt gccggggatc ggcggctggc
     1381 tcgcggcggt ctcgtagaag ttcttgatcg tggtgacctt ggcgcgcgac agctggcgct
     1441 ggaacgccgc gtcggtggcc tcgatccggg agatgaaggc ggcgacctcg tcgccctcgt
     1501 cgaccgcctc cccggcgagg ctgggctcgc cctcggcgta gaaccggctg atgaagcgga
     1561 ccttgtcgag gatgtcatcc gccgggtagg cgcaaaagta gaagacgctg tccttctgcc
     1621 ggatctgggt cgcgagcacg ggccagaact ataccgctac cagatgacac actgctttgc
     1681 ggggaccatc ttcgagccgg ccttgcagcg gaatgccctg gcaaaatccg gggtcgccgc
     1741 cagcgcgccg ttgacccgcc agcgcggcgg cgcgtggacg tcgaccatcg cgagcagccg
     1801 ctcgtagtcg gggcgcgcct tggcgcacca cgcctggccg aacccgagga agaactgctg
     1861 gtcctcggtg aagccgccgg cgatcacggt gtcgggcgcc ccgccgcgca gctggtggag
     1921 cgccgcgagc gcgagcttga cgccgccgat gtcggcgatg ttctccccga cggtgtgggc
     1981 gccgttgagg cgggtgccgc cgctgacctc gtagccgctg tactggtcga tcacgcaccg
     2041 ggtgcgctgc tggaactggc gctcggtgtc gggctgccac cagctggcga ggttgccggc
     2101 ggcgtcgtac tgcgcgccct ggtcgtcg
//
LOCUS       DASVOR010001971         4803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001971 DASVOR010000000
VERSION     DASVOR010001971.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1238)
                     /locus_tag="VF516_36590"
     CDS             complement(<1..1238)
                     /locus_tag="VF516_36590"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113324.1"
                     /translation="MIQVGQVLDKYELLERVGQGGMAIVYRGIDRQLKRVVAIKVLHK
                     HLADYQEARDRFEREAQAVAKLRHENILEIFDYSGAEAAEVAGSSYIVTEFIDGKTLK
                     QQVTDRPITYPEIGAMIVLQICRALAHAHAAGILHRDVKPENIMIRADGVVKLMDFGI
                     SHMVDLERLTVTGQLLGSPAYMAPEHVEGRPLDFRTDVFAAGIVLYQLTVGKLPFEGK
                     NPHEVLKRIAECRFVDPRQANPRIGNRLGKIILRAMAAQPADRFPAIGEMVLALEGYL
                     EETGLAPDKVASELGRYFQAPAAYELALRERMIDHLTRRGQAHLAQDSRATALDVFDR
                     VLTLDPTNPRVLEILDGINRRKRRRSHLIAAAGAAVLATCAWMVHRNAQPPPAVALPE
                     IDAPGTQPVPARRTDVSLIEL"
     gene            1573..2049
                     /locus_tag="VF516_36595"
     CDS             1573..2049
                     /locus_tag="VF516_36595"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8113325.1"
                     /translation="MSSTQVHRRIRAPRDRVYRALLDARAVETWMVPDGMTARVHEFD
                     PREGGAFRVSLTYDAPTGTGKTTPHTDTYHGRFVKLVPDEQVVRVVEFETSDPALHGE
                     MTDTITLRDADGGTEVIGRHDGLPPGVAPADNELGWRMALDKLAALVEAPPPEPRP"
     gene            2087..2158
                     /locus_tag="VF516_36600"
     tRNA            2087..2158
                     /locus_tag="VF516_36600"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2119..2121,aa:Val,seq:gac)
     gene            3012..3761
                     /gene="infC"
                     /locus_tag="VF516_36605"
     CDS             3012..3761
                     /gene="infC"
                     /locus_tag="VF516_36605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEX8113326.1"
                     /translation="MNGRPPGRFDRRRPEQGLRINHRIRVPEIRVILEEEQLGIMPTH
                     EALRLAEEKGLDLVEISPRAFPPVCRIMDYGKYKYEEAKKKQQQRKKASTVETKEIKF
                     RPKTEEHDMDFKVKHVRRFLEGGNKVRLAVVFRGREITHPQTGMKVLNRVVELCTDIA
                     TVEATPNMEGRRMIMVIAPKPGVVRKAQEARKAAAAAAQLAAVAQKKDQPAGKRPPEA
                     EVEDDDLDEDAINADVDDDDEDEAADEVAKA"
     gene            3887..4087
                     /gene="rpmI"
                     /locus_tag="VF516_36610"
     CDS             3887..4087
                     /gene="rpmI"
                     /locus_tag="VF516_36610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEX8113327.1"
                     /translation="MPKVKTHSGAKKRFKRTGTGKFKFQHVFKRHLLTNRSKKRKRQA
                     RAGAYVPDAHHHQIEKLLPYKE"
     gene            4097..4435
                     /gene="rplT"
                     /locus_tag="VF516_36615"
     CDS             4097..4435
                     /gene="rplT"
                     /locus_tag="VF516_36615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEX8113328.1"
                     /translation="MPRAKRGFKARRRRNRVLKAASGFRGKRSSCHGIANEAVQHAWM
                     HMYRSRKVRKRDFRALWIQRINAAARGLGHSYSKLMGKLGTAGIALNRKMLAEIAISD
                     PVAFEKIVRA"
     gene            4543..>4803
                     /locus_tag="VF516_36620"
     CDS             4543..>4803
                     /locus_tag="VF516_36620"
                     /inference="COORDINATES: protein motif:HMM:NF014909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEX8113329.1"
                     /translation="MPTASELIANLERLGDEFVAAVAPLGDEQAIRAAQAGYLGKKGK
                     LSEVMKELGRLPAGDRPAVGAAANKVKQAIEDRVAARLGELAD"
BASE COUNT          801 a         1601 c         1699 g          702 t
ORIGIN      
        1 agctcgatga gcgagacgtc ggtgcggcgg gcggggaccg gctgggtgcc gggcgcgtcg
       61 atctccggca gcgccaccgc cggcggcggc tgggcgttgc ggtggaccat ccacgcgcag
      121 gtcgcgagca cggcggcgcc cgcggcggcg atcaggtggc tgcgccgccg cttgcgccgg
      181 ttgatgccgt cgaggatctc gagcacccgg gggttcgtgg gatcgagcgt gagcacgcgg
      241 tcgaacacgt cgagcgcggt cgcgcggctg tcctgggcga ggtgggcctg gccgcgccgg
      301 gtgaggtgat cgatcatccg ctccctgagc gcgagctcgt aggcggcggg ggcctggaag
      361 tagcggccga gctcgctggc gaccttgtcg ggggccagcc cggtctcctc gaggtagccc
      421 tccagcgcga gcaccatctc gccgatcgcg gggaaccgat cggcgggctg cgccgccatc
      481 gcgcgcagga tgatcttgcc gagccggttg ccgatccgcg ggttggcctg gcgcgggtcg
      541 acgaaccggc actcggcgat ccgcttgagc acctcgtgcg ggttcttgcc ctcgaacggc
      601 agcttgccga cggtgagctg gtacagcacg atgccggccg cgaacacgtc ggtccggaag
      661 tcgagcggcc ggccctcgac gtgctccggc gccatgtagg ccggcgagcc gagcagctgc
      721 ccggtcacgg tgagccgctc gaggtcgacc atgtgcgaga tcccgaagtc catcagcttc
      781 acgaccccgt cggcacggat catgatgttc tcgggcttga cgtcgcggtg caggatgccg
      841 gcggcgtggg cgtgcgccag cgcgcggcag atctgcagca cgatcatcgc gccgatctcg
      901 gggtaggtga tcgggcggtc ggtgacctgc tgcttgagcg tcttgccgtc gatgaactcg
      961 gtgacgatgt agctcgagcc ggcgacctcg gcggcctcgg cgcccgaata gtcaaagatc
     1021 tcgaggatgt tctcgtggcg cagcttggcg actgcctggg cctcgcgctc gaaccgatcg
     1081 cgcgcctcct ggtagtccgc gaggtgcttg tggagcacct tgatcgccac cacccgcttg
     1141 agctgccgat cgatgccgcg atagacgatg gccatcccgc cttgcccgac gcgctcgagc
     1201 aattcgtact tgtcgaggac ctggccgacc tggatcacgt gctcacgaaa gccatgacag
     1261 cagtgtcacg gcgcagctct ggatggatcg gaaccggcgc gaggcccgac cgcgttgtct
     1321 aaccccataa gcttacgcct gttctgacgc acacagcaac caattggatg ttccgggcac
     1381 tgacgttgca gccagagtcc tggacctggc aaatctcgat caggatgctc ggtgatcgcg
     1441 cgcacgcgcc gcgatcggcc cggatgctac gggaactcca cctcaccgac gctgtgccgc
     1501 cgcgggagcc agggctcgat ccgccgatct cgcagcgcgg cagccggagg gcgcacgtgg
     1561 caaggtccgg ccgtgagctc gacccaggtc caccgccgca tccgtgcgcc ccgcgaccgt
     1621 gtgtaccgtg cgctgctcga cgcgcgcgcg gtcgagacct ggatggtccc cgacggcatg
     1681 accgcccgcg tccacgagtt cgatccgcgc gaaggcggcg cgttccgggt gtcgctgacg
     1741 tacgacgcgc cgaccgggac cggcaagacg acgccgcaca ccgacacgta ccacggccgg
     1801 ttcgtcaagc tggtgccgga cgagcaggtc gtccgcgtcg tcgagttcga gaccagcgat
     1861 ccggcgctgc acggcgagat gaccgacacg atcacgctgc gcgacgcgga cggcgggacc
     1921 gaggtcatcg gacggcacga cggcctgcca ccgggcgtgg ccccggccga caacgagctc
     1981 ggctggcgga tggcgctcga caagctcgcc gcgctcgtcg aggcgccgcc accggagccg
     2041 cggccttgac ttatcgcagt cgatcgcgta cttgaagtgc ctctccaggc acgtagctca
     2101 gtgggagagc actaccttga cacggtaggg gtccgcggtt caatcccgcg cgtgcctacg
     2161 acccgcgcac cgtcggcgct cgacggcgat ggccgggaag cagcgatccc gggaacgctg
     2221 gcttccctcg ccggcttcca tcgcctcagg gatccgagcg acgtgatcga tcaccggcga
     2281 tcggggcgtc gtcgcggccg ggatcggcgt cgtggtcggg ttgcgatgtg cgttctggag
     2341 aacgcgatgc gcgcgcgcaa gctcgcgacg atgcgcagtc tggacgcggt atgggcgatg
     2401 caacccgggg cacgggccat cgcaacggcc ccaggaggtc tatgtcgatc acgaagctga
     2461 agacgctcgg gagctgggag ccgccgcgct ggcgggaggg tgcggtgggg gaagggccga
     2521 cgagatttcg cggacggatc atcaccggga tgctgaccgg cggtgatccg gatgcagacg
     2581 acgctgcggc cggggagccg ggcgaggcga ggaaccgtta gcctggatgg ttcacctcga
     2641 cgtcgtggtg aatgatccgg gcctcagccc ggtgcgcggg cgtcgcgccg tgcgctgccg
     2701 aggatgcctc gcgcggccac ggcgcgatgg tcattccgga gcgttgcgtc cggatccccg
     2761 gcaatgcgct gcagggcgct ccgcggtcgg tgtaggcgga tgggcagggc ggctgcgatg
     2821 ccggcccctc cccggtcagg gaggcgtaac tcccgatgtc acaaggagtt ggggtgatct
     2881 gcgggacgca ccttgtcacc cgaaattcgt gacatcgggc tcccacccct tcgttgggcg
     2941 gggtacaacc tgctgcctgc tagacgcaga tggagatccc agagacacat gagcatgagt
     3001 gacgtgtacc aatgaacggc cggccgcctg gacgtttcga tcgtcgtcgc cccgagcagg
     3061 gcctgcgcat caaccaccgc atccgcgtcc cggagatccg ggtcatcctc gaggaagagc
     3121 agctcggcat catgccgacc cacgaagcgc tgcggctcgc cgaggagaag ggcctcgatc
     3181 tggtcgagat ctccccgcgc gcgtttcctc cggtctgccg gatcatggac tacggcaagt
     3241 acaagtacga ggaagccaag aagaagcagc agcagcgcaa gaaggcgtcg acggtcgaga
     3301 ccaaggagat caagttccgg cccaagaccg aggagcacga catggacttc aaggtgaagc
     3361 atgtccggcg cttcctcgag ggcggcaaca aggtccggct cgccgtggtg ttccgcggtc
     3421 gcgagatcac gcacccgcag accggcatga aggtgctcaa ccgcgtcgtc gagctgtgca
     3481 ccgacatcgc gaccgtggag gcgaccccga acatggaggg ccgccgcatg atcatggtca
     3541 tcgcgcccaa gcccggcgtg gtgcgcaagg cgcaggaggc ccgcaaggcc gccgcggcgg
     3601 cggcccagct cgccgcggtc gcccagaaga aggaccagcc cgctggcaag cggccgccgg
     3661 aggccgaagt cgaagacgac gatctcgacg aggacgcgat caacgcggac gtcgacgacg
     3721 acgacgagga cgaggcggcc gacgaggtcg ccaaggccta gccgagccca ggcagcgccg
     3781 gcgtttgccg gatctcgccg ggttgcggtc atttggccgc gaaatcgctt gtctttgccc
     3841 ggggcatcgg gcataagaca cctccttccg ttttcgcgga cccgccatgc ccaaggtcaa
     3901 gactcactcc ggtgcgaaga agcgtttcaa gcgtaccggc accggcaagt tcaagtttca
     3961 gcatgtgttc aagcgccacc tgctgaccaa ccgcagcaag aagcgcaagc gccaggcccg
     4021 cgccggcgcc tacgtgccgg acgctcatca tcaccagatc gagaagctcc tgccgtacaa
     4081 ggagtgagga ttcgccatgc cccgcgccaa acgaggattc aaggctcgcc gccgtcgcaa
     4141 ccgcgtgctc aaggccgcca gcggcttccg cggcaagcgc tccagctgcc acggcatcgc
     4201 caacgaggcg gtgcagcacg cgtggatgca catgtaccgc tcgcgcaagg tccgcaagcg
     4261 cgacttccgc gcgctgtgga tccagcggat caacgccgcg gcccgcgggc tcggccacag
     4321 ctactccaag ctgatgggca agctcggcac cgccggcatc gcgctcaacc gcaagatgct
     4381 cgccgagatc gcgatcagcg atccggtcgc gtttgaaaag atcgtccgcg cctgacccga
     4441 gccgctcggc ccgattctga cggcggctcc gcaagagtcg ccgatttgct ttttggagcg
     4501 agtaaccgcc gggggcgcct cggcgagcga agtgaacccc cgatgccgac tgccagcgag
     4561 ctgatcgcca acctcgaacg cctgggcgac gagttcgtcg ccgcggtcgc cccgctcggt
     4621 gacgagcagg cgatccgcgc ggcccaggcc ggctacctgg gcaagaaggg caagctgtcc
     4681 gaggtcatga aggagctcgg ccggctgccg gcgggcgatc ggccggcggt cggcgccgcg
     4741 gccaacaagg tcaagcaggc gatcgaggac cgggtcgcgg cgcggctcgg cgagctcgcc
     4801 gat
//
LOCUS       DASVOR010001972         3285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_459037,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001972 DASVOR010000000
VERSION     DASVOR010001972.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3285
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459037"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1319
                     /gene="mraY"
                     /locus_tag="VF516_36625"
     CDS             168..1319
                     /gene="mraY"
                     /locus_tag="VF516_36625"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEX8113330.1"
                     /translation="MLFHLLYGGLGDRIGWLRVFRYTSSRILLAAITALLLSFVIGPW
                     FIEQLKSRQIGETIRSDGPTTHKKKAGTPTMGGSLILFCLAVSTLLWCDLRNQFVWLS
                     LTVTVAFGAIGFADDYVKLIKKDKRGVSGGLRLGLELAIAGAAMAYVFYSRLMPDDVR
                     LHLQLPFTNFYEQGLILPPWLYATFAALVVVGTANAVNLTDGLDGLAIGPTIMNAGTF
                     LIFAYIAGVSTTILAHNGHEVNLAQYLHVAHISGAEELAVFAAALFGAGVGFLWYNAY
                     PAQVFMGDVGALSLGGSIGMLAVLTKNEIVLLIVGGLFVIETLSVIIQRGAFKITKRL
                     TGTGKRVFAMAPIHHHYEKKGWDEPKIIVRFWLVSLLFALMALGTLKLR"
     gene            1328..2827
                     /gene="murD"
                     /locus_tag="VF516_36630"
     CDS             1328..2827
                     /gene="murD"
                     /locus_tag="VF516_36630"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEX8113331.1"
                     /translation="MTAGSPHLHGDLRGKTIVVVGLAQTGIAVAKFCARRGAHVVVTD
                     GKPADQLAGPIAQLAGVPVSWQLGGHDAHSFTTADLVVMSPGVPTLPEMVAARAAGVE
                     VIAEIELAYRFLDPGATLIAITGTNGKSTTTALTGALCAASGAPTFCGGNLGNMPLID
                     AVDHPANAPGGLIVAEVAAFMLENCSSFRARAGVLTNITEDHLDRFGTMARYAEMKGR
                     IWDTQRPDDLAIGNAADPWTMAETAGIPSRLVTFDARPGAAAVRGAVLSPDRREIVLR
                     GVTDDWGTREERYPTSDLVIVGNHNLENAMGAYLAARGVGVPIDAVRAGARAYRPLPH
                     RMELVGDAGGIQYYDDSKGTNVASVAASVRGFPRPLALIAGGVDKGGSYQPMLEALDG
                     VCKGIVLIGQAAPLIRAAAAAHGVGYPVVDATDMHDAVRRATELCGPGDAVVLSPACS
                     SYDMFQNFGHRGQVFRDAVAQVTATRPSPAASRDRPGDGGASGAGGSRG"
     gene            complement(2742..2993)
                     /locus_tag="VF516_36635"
     CDS             complement(2742..2993)
                     /locus_tag="VF516_36635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113332.1"
                     /translation="MQWNRVLPLIAVVVLVFGAPALIYAVQGRPMWQREIAVYGAMLA
                     FAWAIGYRRELLSLGSRLLRWHRRRRADRAMPRATVVSR"
     gene            complement(3038..>3285)
                     /locus_tag="VF516_36640"
     CDS             complement(3038..>3285)
                     /locus_tag="VF516_36640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113333.1"
                     /translation="GDRVVVPRPAILSVVRVLAALQLCVWLFALWLAALGVLCIAAVD
                     LRVWLEASAAAGALHLAVVAALVLRKGVRLTSGAVAP"
BASE COUNT          483 a         1237 c         1111 g          454 t
ORIGIN      
        1 gtcgaggccg ccgcccgtgc gttcgcccgg accggccccg gcgactggat cctgctcacg
       61 gcgtcgcgcg gcatgcgact cgagcgcgtg ctcgacgcga tccgcgcggc gacagcgagc
      121 ccggcacccg gaatccaccc cgaggtcccc accgagggaa cgacctgatg ctgttccacc
      181 tgctgtacgg cggcctcggc gaccggatcg gctggctccg cgtgttccgc tacacgtcga
      241 gccggatcct gctggccgcg atcaccgcgc tgctcttgtc gttcgtgatc ggcccgtggt
      301 tcatcgagca gctcaagtcg cgccagatcg gcgagacgat ccgcagcgac ggcccgacga
      361 cccacaagaa gaaggccggc acgccgacga tgggcggctc gctgatcctg ttctgcctcg
      421 cggtgtcgac gctgctgtgg tgcgacctgc gcaaccagtt cgtgtggctg tcgctgaccg
      481 tgaccgtcgc gttcggcgcg atcgggttcg ccgacgacta cgtcaagctg atcaagaagg
      541 acaagcgcgg cgtgtccggc gggctgcggc tcggcctcga gctcgcgatc gccggcgcgg
      601 cgatggccta cgtgttctac tcgcggctca tgcccgacga cgtgcggctg cacctgcagc
      661 tgccgttcac caacttctac gagcagggcc tgatcctgcc gccgtggctg tacgcgacgt
      721 tcgccgcgct ggtcgtggtc ggcaccgcca acgcggtcaa cctgaccgac ggcctcgacg
      781 gcctggcgat cggcccgacg atcatgaacg ccggcacgtt cctgatcttc gcgtacatcg
      841 ccggcgtatc gacgacgatc ctcgcccaca acggccacga ggtgaacctc gcgcagtacc
      901 tccacgtggc gcatatctcg ggcgccgagg agctcgcggt gttcgcagcc gcgctgttcg
      961 gcgccggggt cggcttcctg tggtacaacg cgtaccccgc ccaggtgttc atgggcgacg
     1021 tcggcgcgct gtcgctcggc ggctcgatcg gcatgctcgc cgtgctgacc aagaacgaga
     1081 tcgtgctcct gatcgtcggc gggctgttcg tgatcgagac cctgtcggtg atcatccagc
     1141 gcggcgcgtt caagatcacc aagcggctga ccggcaccgg caagcgggtg ttcgcgatgg
     1201 cgccgatcca ccaccactac gagaagaagg gctgggacga gcccaagatc atcgtccggt
     1261 tctggctggt gtcgctgctg ttcgcgctga tggcgctcgg caccctcaag ctcaggtagc
     1321 gacgacgatg accgccggat ctcctcacct ccatggcgat ctccgcggca agacgatcgt
     1381 cgtcgtcggc ctcgcccaga ccggcatcgc ggtcgcgaag ttctgcgccc ggcgcggcgc
     1441 gcacgtcgtg gtcaccgacg gcaagcccgc cgatcagctc gccggtccga tcgcccagct
     1501 cgccggcgtg ccggtgtcct ggcagctcgg cggccacgac gcgcacagct tcaccaccgc
     1561 cgacctcgtc gtgatgtcgc ccggcgtgcc gaccctgccc gagatggtcg ccgcccgcgc
     1621 cgccggcgtc gaggtcatcg ccgagatcga gctcgcctat cgtttcctcg atccgggcgc
     1681 cacgctgatc gcgatcaccg gcaccaacgg caagtcgacc accaccgcgc tgaccggcgc
     1741 gctgtgcgcg gcgtcgggcg ccccgacgtt ctgcggcggc aacctcggca acatgccgct
     1801 gatcgacgcg gtcgaccacc ccgccaacgc gcccggcggt ctgatcgtcg ccgaggtcgc
     1861 ggcgttcatg ctggagaact gctcgagctt ccgcgcccgc gccggcgtgc tcaccaacat
     1921 caccgaggac cacctcgacc ggttcggcac gatggcgcgc tacgccgaga tgaaggggcg
     1981 gatctgggac acccagcgcc ccgacgacct cgcgatcggc aacgccgccg acccctggac
     2041 gatggccgag accgccggga tcccgtcgcg gctggtcacg ttcgacgcgc gccccggcgc
     2101 ggccgccgtg cgcggcgcgg tgctgtcgcc cgaccgccgc gagatcgtgc tgcgcggcgt
     2161 gaccgacgac tggggcaccc gcgaggagcg ctacccgacc tcggacctcg tcatcgtcgg
     2221 caaccacaac ctcgagaacg cgatgggcgc ctacctcgcg gcgcgcgggg tcggcgtccc
     2281 gatcgatgcg gtccgcgccg gcgcgcgcgc ctaccggccg ctgccgcacc gcatggagct
     2341 ggtcggcgac gccggcggca tccagtacta cgacgacagc aagggcacca acgtcgcctc
     2401 ggtcgccgcc tcggtgcgcg gcttcccgcg cccgctggcg ctgatcgccg gcggcgtcga
     2461 caagggcggc tcgtaccagc cgatgctcga ggcgctcgac ggcgtgtgca agggcatcgt
     2521 gctgatcggc caggccgccc cgctgatccg cgccgccgcc gcggcgcacg gcgtcggcta
     2581 cccggtcgtc gacgccaccg acatgcacga cgcggtccgg cgcgccaccg agctgtgcgg
     2641 ccccggcgac gccgtggtgc tgtcgccggc gtgctcgagc tacgacatgt tccagaactt
     2701 cggccaccgc ggccaggtgt tccgcgacgc agtcgcccag gtcaccgcga cacgaccgtc
     2761 gcccgcggca tcgcgcgatc ggcccggcga cggcggtgcc agcggagcag gcggctcccg
     2821 aggctgagca gctcgcggcg gtagccgatc gcccaggcga acgccagcat cgccccgtac
     2881 acggcgatct cgcgctgcca catcggccgg ccctggacgg cgtagatcag tgcaggcgcg
     2941 ccgaagacca gcacgaccac cgcgatcagt gggagcacgc ggttccactg cacggcccca
     3001 ggatagcaaa ctcacgctgg cgacgcggcc cggccgctca gggcgcgacg gcgcccgatg
     3061 tcagccggac gcccttgcgg aggaccagcg cggcgaccac cgcgaggtgg agggcgccgg
     3121 ccgccgcgct ggcctcgagc cacacccgca ggtcgaccgc cgcgatgcac agcacgccca
     3181 gggcagcgag ccacagcgcg aacagccaca cgcacaactg cagcgccgcg agcacacgca
     3241 cgacggacag gatagcaggt cgtggcacca cgactcgatc gccgc
//
LOCUS       DASVOR010001973         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_459055,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001973 DASVOR010000000
VERSION     DASVOR010001973.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459055"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="VF516_36645"
     CDS             <1..293
                     /locus_tag="VF516_36645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113334.1"
                     /translation="GPIHLDKTAALFDAGCTGGDAASCAALAAMYDLGKGRPRDLARA
                     TQLYDRACSAKLASACVQAGHLYHRAGDDETAWRRYDQGCRDGDALACRLAQ"
     gene            complement(304..1005)
                     /locus_tag="VF516_36650"
     CDS             complement(304..1005)
                     /locus_tag="VF516_36650"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8113335.1"
                     /translation="MTAPVCAVVGVGPGNGAAFARRFAADGYAVALLARSLATSRELA
                     AVLPGARAWECDVGDAASVQRAFDAIRGELGDPEIVIYNAGSGLFGSFDDVTAADFEA
                     SWRVNALGAFLVAKQVVPAMKQAGRGNFVMIGATASRRGGIRSAAFAPAKAAQRSLAE
                     SMARSLGPAGIHVSLIVIDGVVDLPRTRQRLPDKPDAFFVRPDDVAATAAFLVGQPRS
                     AWTFELEARPFGETW"
     gene            1157..>1802
                     /locus_tag="VF516_36655"
     CDS             1157..>1802
                     /locus_tag="VF516_36655"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8113336.1"
                     /translation="MSLRATYFGWQGWLFETDRAAVAIDPLLVDQVGRGPRHTRIDFV
                     FREAREVNLDRLPPLDALVLSHEHEDHFSVPTLVRLDRRIPLYVSRRVSAAARGLLTE
                     LGFAVRYVDHGDTLAFGDLEVRLFGADQTASTASFDEWDTLAYLVRQRDGAGAMFSNV
                     DISISHEMAEAIRDIAARDRGLLMFVRGEVSLSSGAPPTVADGEHPRGAAATPIE"
BASE COUNT          251 a          664 c          664 g          223 t
ORIGIN      
        1 gggggccgat ccacctcgac aagaccgcgg cgctgttcga cgccggctgc acgggcggcg
       61 acgcggcctc ctgcgccgcg ctggccgcga tgtacgacct gggcaagggc aggccgcgcg
      121 acctcgcccg cgcgacccag ctctacgatc gcgcgtgcag cgccaagctc gcgtcggcct
      181 gcgtccaggc cggtcacctg taccaccggg cgggcgacga cgagacggcg tggcggcgct
      241 acgaccaggg ctgccgagac ggcgacgcgc tggcgtgtcg cctcgcccag tgaccggccc
      301 cgctcaccag gtctcgccga acgggcgggc ctcgagctcg aacgtccacg ccgagcgcgg
      361 ctggccgacc aggaatgccg cggtcgcggc gacgtcgtcg ggccggacga agaacgcgtc
      421 cggcttgtcg ggcaggcgct ggcgggtgcg cggcagatcg accacgccgt cgatcacgat
      481 cagcgacacg tgaatgccgg cgggcccgag cgagcgggcc atcgactcgg cgagcgagcg
      541 ctgcgccgcc ttggccgggg cgaacgccgc gctgcggatc ccgccgcgcc gcgatgcggt
      601 cgcgccgatc atcacgaagt tgccgcggcc ggcctgcttc atcgccggca cgacctgctt
      661 ggcgaccagg aacgcgccga gcgcgttgac ccgccagctc gcctcgaagt cggcggcggt
      721 gacgtcgtcg aagctgccga acaggccgga gccggcgttg tagatcacga tctcgggatc
      781 gccgagctcg ccgcggatcg cgtcgaacgc gcgctggacc gacgcggcgt caccgacgtc
      841 gcactcccag gcgcgcgcgc ccggcagcac ggccgcgagc tcgcgcgagg tggccagcga
      901 gcgcgcgagc agcgcgacgg cgtagccatc cgccgcgaac cggcgcgcga acgccgcgcc
      961 gttgccggga ccgacaccca ccactgcgca gaccggtgct gtcatgcgcc gaacatacga
     1021 cgcagccggc tgcgagcaag ccggcatgcc gcgctgtcgg atcgtgtgga tgtatgtcat
     1081 ggcgcgcggc ggtccgcgca ggatgtgcgc cgacgccggc cgccgcgcgc gcggcgaatc
     1141 gtgaaatgat cacgcgatga gtttacgggc gacgtacttc ggatggcagg gctggctgtt
     1201 cgagaccgat cgcgcggcgg tggcgatcga tccgctgctc gtcgaccagg tcgggcgcgg
     1261 accgcgccac acccggatcg acttcgtgtt ccgcgaggcg cgcgaggtca atctcgaccg
     1321 gctgccgccg ctcgacgccc tggtgctcag ccacgagcac gaggaccact tcagcgtgcc
     1381 gacgctggtg cggctcgatc gccggatccc gctctacgtg tcgcgccggg tgtcggcggc
     1441 ggcgcgcggc ctgctgaccg agctcggctt cgcggtccgc tacgtcgacc acggcgacac
     1501 gctggcgttc ggcgacctcg aggtccggct gttcggcgcc gaccagaccg ccagcaccgc
     1561 gagcttcgac gagtgggaca cgctggccta cctggtgcgc cagcgcgacg gcgccggcgc
     1621 gatgttctcc aacgtcgaca tctcgatcag ccacgagatg gccgaggcga tccgcgacat
     1681 cgccgcgcgc gaccgcgggc tcctgatgtt cgtgcgcggc gaggtctcgc tgtcgagcgg
     1741 cgcgccgccg accgtcgccg acggcgagca cccgcggggc gccgccgcca cgccgatcga
     1801 gg
//
LOCUS       DASVOR010001974         4349 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_282837,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001974 DASVOR010000000
VERSION     DASVOR010001974.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4349
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282837"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VF516_36660"
     CDS             <1..219
                     /locus_tag="VF516_36660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113337.1"
                     /translation="TNFAALRALATEGIQRGHMALHARGLARAAGATGELIDQVASEL
                     AATGDIKADRAREVFARLCAPRMVKDSP"
     gene            216..1178
                     /gene="hpnC"
                     /locus_tag="VF516_36665"
     CDS             216..1178
                     /gene="hpnC"
                     /locus_tag="VF516_36665"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEX8113338.1"
                     /translation="MIQRLGVARSPALAVPLPASLEQGACYRYCETLVRARHHNYPVA
                     SMFARSQLRKHIFALFAFARVADDFADEAAYEGRRARELDRWEEQLHAAYRGHADHPV
                     FVALADTVDKFALPITEFTELLSGFRTDLERRRYATFDELRSYTRQAAEPIGRLLLYI
                     AGYRAPELHAYADDLSTAVAVARLIQDVSDDWQRGRVYLPAEDLRHFGVTEADIEARR
                     VTKAVGYLVHYEVARTRALFERARPLVDLAGSDLAVELALTWHGGMRILDKIAATGER
                     LLAHRPQLGQVDKAIVLARSVAWRGETLPPRTLHLLNRLTASDA"
     gene            complement(1196..1666)
                     /locus_tag="VF516_36670"
     CDS             complement(1196..1666)
                     /locus_tag="VF516_36670"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8113339.1"
                     /translation="MLVEVVFQYRTLMGKCELGVGLDWDEIEQVTAIESAFEPTSDDR
                     RMKTGRRFRRESVKLSALLRGDRINDRVEVVEIGPGGAVVRNAPFIAHGEHVEIVIED
                     GDRSYRFGAEGVWLKDDGDDYRVGLALIGMPVCLHTVAVSRHEVDLVDRIGAAA"
     gene            1897..3258
                     /locus_tag="VF516_36675"
     CDS             1897..3258
                     /locus_tag="VF516_36675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEX8113340.1"
                     /translation="MDLFAASAKKRHVGQPLAERMRPRSLDELVGQRHLVGPGRILSG
                     LTPGRPIPSLILWGPPGSGKTTLARLLGETLKAELNALSAVDAGVKDVREAVAKAAAR
                     RDQFGARTLLFIDEIHRFSKTQQDALLPHVEAGTVTLIGATTENPSFGVVAALLSRCR
                     VVRLEALSDPELGELAQRAIADRARGLGALNGTLAAEVTAQLVAAAAGDARRLYSVLE
                     VAAELARRSAPGAPGAPGPDDAALAAVTAEHIAEAAQGRNLLYDKAGDEHYGVVSVFI
                     KSMRGSDPDAAVYWMTRMIEAGEDPRFVLRRMVIFAAEDIGNADPQALGVAVAALQAV
                     ELVGLPEGVLPMTQAVSYLALAPKSNTALTAYAAARKLVTERGALPVPARYRPGSTAL
                     DRAMGHGQGYKYPHDFAGHYVPEDYLPDELAGERIYRPSDSGFEAELSRRLAAIAERK
                     RSR"
     gene            complement(3394..3654)
                     /locus_tag="VF516_36680"
     CDS             complement(3394..3654)
                     /locus_tag="VF516_36680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113341.1"
                     /translation="MALDPGIEELFLGIAHALFVNRLHVLRLTEIVRLGIRPDPNDQN
                     MDVPPEVDRELINQAFAYVQRHFPPSFTPKLDAARARWVRLA"
     gene            complement(3724..>4349)
                     /locus_tag="VF516_36685"
     CDS             complement(3724..>4349)
                     /locus_tag="VF516_36685"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8113342.1"
                     /translation="LLSPQHWAYSGDVPRYDHDPARARRLLDEAGLRDPDGDGPAPRL
                     RLVYKTSSDAFRIAIARVIAAQLAEVGIAVEIRSFEFATFFADVKRGAYQLASMQTTD
                     ITEPDFYFMYFHSSWIPSAPRFEGFNRWRYRSAVVDRLTAEGRHELDRDRRRRIYAEV
                     QRQVAEDVPIVPLWHEDNVVLSSADVRGYTLTPNARLTGLRGLHKQR"
BASE COUNT          640 a         1582 c         1510 g          617 t
ORIGIN      
        1 accaactttg ccgcgctgcg cgcgctggcg accgagggca tccagcgcgg ccacatggcc
       61 ctccatgccc gcggcctggc ccgcgccgcc ggcgccaccg gcgagctgat cgaccaggtc
      121 gccagcgagc tcgccgccac cggcgacatc aaggccgacc gcgcgcgcga ggtgttcgcg
      181 cgcctgtgcg ccccccgcat ggtaaaggat agtccgtgat ccaacgcctg ggagtcgccc
      241 gttcccccgc gctcgccgtg ccgctgcccg cctcgctcga gcagggcgcg tgctaccgct
      301 actgcgagac gctggtccgc gcccgccacc acaactaccc ggtcgcgtcg atgttcgcgc
      361 gcagccagct gcgcaagcac atcttcgcgc tgttcgcgtt cgcccgcgtc gccgacgact
      421 tcgccgacga ggcggcctac gagggccggc gcgcccgcga gctcgaccgc tgggaggagc
      481 agctccacgc cgcctaccgc ggccacgccg atcacccggt gttcgtcgcg ctcgccgaca
      541 cggtcgacaa gttcgcgctg ccgatcacgg agttcaccga gctgctgtcc ggcttccgga
      601 ccgacctcga gcgccggcgc tacgcgacgt tcgacgagct gcgcagctat acccggcagg
      661 ccgccgagcc gatcggccgg ctgctgctct acatcgccgg ctaccgcgcg cccgagctcc
      721 acgcctacgc ggatgacctg tcgaccgcgg tcgcggtcgc gcggctgatc caggacgtgt
      781 ccgacgactg gcagcgcggc cgggtctacc tgccggccga ggacctccgg cacttcggcg
      841 tcaccgaggc cgacatcgag gcccgccggg tgaccaaggc cgtcgggtac ctggtgcatt
      901 acgaggtcgc gcggacccgc gcgctgttcg agcgggcgcg cccgctggtc gacctcgccg
      961 gctccgatct cgcggtcgag ctcgcgctga cctggcacgg cggcatgcgg atcctcgaca
     1021 agatcgccgc gaccggcgag cggctgctcg cccatcgccc acagctcggt caggtcgaca
     1081 aggcgatcgt gctggcccgc tcggtggcgt ggcgcggcga gacgctgcca ccgcgcacgc
     1141 tgcacctgct caaccggctg acggcgtctg acgcttaggc ggacggtcag cgcggctacg
     1201 ccgcagcgcc gatccggtcg accaggtcga cctcgtggcg gctgaccgcg accgtgtgca
     1261 ggcacaccgg catgccgatc agcgccaggc cgacccggta gtcatcgccg tcgtccttga
     1321 gccacacgcc ctcggcgccg aaccggtagc tgcggtcgcc gtcctcgatg acgatctcga
     1381 cgtgctcgcc gtgcgcgatg aacggcgcat tccgcaccac cgcgccgccg ggtccgatct
     1441 cgacgacctc gacccggtcg ttgatccggt cgccgcgcag caaggcgctg agcttgaccg
     1501 actcgcggcg gaaccggcgg cccgtcttca tgcggcggtc gtcggaggtc ggctcgaacg
     1561 cggactcgat cgcggtgact tgctcgatct catcccagtc aagcccgaca cccagctcgc
     1621 atttccccat cagggtgcga tactgaaaca cgacctcgac gagcatgtgt cagggaaata
     1681 gcaggcggca tgccacgcac ggctccgcgg tcagtcgagg taagagagcg aaagttcgat
     1741 ggtgactcag cggaggaggg gcaaggtagc cgggtgtctc gtcaccatgt acgggtctcg
     1801 tactccaccg cacaagatgt gcacacgacc ggcgtaacct ggcgatacta cgcagcgcca
     1861 tcgccgggct gcccggagta gagtatccac gccgcgatgg acctgttcgc cgccagcgcc
     1921 aagaagcgcc acgtcggtca gccacttgct gaacgaatgc ggccacgtag cctcgacgag
     1981 ctggtcggcc agcgccacct ggtggggccg ggacgcatcc tgtcaggtct caccccaggg
     2041 cgccccatcc cctcgttgat cctgtgggga cccccgggga gcgggaagac cacgctcgca
     2101 cgcctgctcg gagaaaccct gaaggcggag ctgaacgccc tctcagcggt cgacgccggg
     2161 gtcaaggatg tccgggaggc ggtggccaag gcggcggcgc ggcgcgatca gttcggcgcg
     2221 cggacgctgc tgttcatcga cgagatccac cggttctcca agacccagca ggacgcgctc
     2281 ttgccccacg tggaggccgg caccgtcacc ctgatcggcg cgaccaccga gaacccgagc
     2341 ttcggggtgg tcgccgcgct gctgtcgcgg tgccgggtcg tgcgtctgga ggcgctgtcg
     2401 gatcccgagc tcggcgagct cgcgcagcgc gcgatcgccg accgcgcgcg cggcctcggt
     2461 gccctgaacg ggacgctcgc cgccgaggtc accgcacagc tggtcgccgc ggccgccggc
     2521 gacgcccgcc ggctgtactc ggtcctcgag gtcgccgccg agctcgcccg ccgttccgcg
     2581 ccgggcgcgc cgggtgcgcc gggaccggac gacgcggcat tggccgcagt caccgccgag
     2641 cacatcgccg aggctgccca gggccgcaat ctgctctacg acaaggccgg cgacgagcac
     2701 tacggcgtgg tgtcggtgtt catcaagagc atgcgcggct cggatcccga cgccgcggtg
     2761 tactggatga cccggatgat cgaggccggc gaggacccac ggttcgtgct gcgccgcatg
     2821 gtgatcttcg cggccgagga catcggcaac gcggatcccc aggcgctcgg cgtcgcggtg
     2881 gccgcgctgc aggcggtcga gctggtcggc ctccccgagg gcgtcctgcc gatgacccag
     2941 gcggtgagct acctggcgct ggcgcccaag tcgaacaccg ccctcaccgc ctacgcggcg
     3001 gcgcgcaagc tggtcaccga gcgcggcgcg ctgccggtcc ccgcgcgcta ccgccccggg
     3061 tcgacggcgc tcgatcgtgc gatgggccac ggccagggct acaagtaccc gcacgacttc
     3121 gccggtcact acgtccccga ggactacctc cccgacgagc tggccggcga gcggatctac
     3181 cggccgagcg actcggggtt cgaggccgag ctgtcgcgcc ggctcgccgc gatcgccgag
     3241 cgcaagcgct cgcgctgatc acctacgcca ggcggaccca gcgcgccctg gcggcatcga
     3301 tcgatagggg gctttcccat aacccccctc tgggggctgg accgacaaag ccggtccatt
     3361 cacccctccg cgcgccgcgt cgcgcggctc tttctacgcc aggcggaccc agcgcgccct
     3421 ggcggcatcc agcttgggcg tgaatgacgg cgggaagtgg cgctgcacgt acgcgaacgc
     3481 ctgattgatg agctcgcggt cgacctcggg cggcacgtcc atgttctggt cgttcggatc
     3541 aggccggatc ccgagtcgga cgatttcggt gagtcggagc acgtgcagtc gattgacgaa
     3601 cagcgcatga gcgatcccga ggaacagctc ttcgattccg gggtccaggg ccatgggagc
     3661 ccccgtacgt atcacaggcg ccgagcgcgt tctagcccgg aaatcgttcg cgacgtcgag
     3721 cggtcagcgc tgcttgtgga ggccgcgcag ccccgtgaga cgtgcgttgg gcgtcagtgt
     3781 gtagccgcgc acgtcggcgc tcgacagcac gacgttgtcc tcgtgccaca gcggcacgat
     3841 cggcacgtcc tcggcgacct ggcgctggac ctcggcgtag atccgccggc gccggtcgcg
     3901 atcgagctcg tggcggccct ccgcggtcag gcggtcgacc accgcgctgc ggtagcgcca
     3961 gcggttgaag ccctcgaacc ggggcgccga cgggatccac gacgagtgga agtacatgaa
     4021 gtagaaatcg ggctcggtga tgtcggtggt ctgcatcgac gccagctggt aggcgccgcg
     4081 cttgacgtcg gcgaagaacg tcgcgaactc gaacgagcgg atctcgaccg cgatgccgac
     4141 ctcggcgagc tgcgccgcga tcacgcgcgc gatcgcgatc cggaacgcgt ccgacgaggt
     4201 cttgtagacc aggcgcagcc gcggcgcggg gccgtcgccg tcgggatcgc gcaggccggc
     4261 ctcgtcgagc agccggcggg cgcgcgccgg gtcgtggtcg tagcgcggca cgtcgccgga
     4321 gtacgcccag tgctgcggcg acagcaggc
//
LOCUS       DASVOR010001975         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_429283,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001975 DASVOR010000000
VERSION     DASVOR010001975.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429283"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2793)
                     /locus_tag="VF516_36690"
     CDS             complement(<1..>2793)
                     /locus_tag="VF516_36690"
                     /inference="COORDINATES: protein motif:HMM:NF013124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043531 - ADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NB-ARC domain-containing protein"
                     /protein_id="HEX8113343.1"
                     /translation="GVIWVAVGRDAQPLAVLQQIALRLGDDLAKYATEAAARQQLAVR
                     LDRAAVLIVLDNIWSRDPVKLVREALGVRCRLLLTAREPSVLEVGAPTVSLGPFSPDE
                     ALAYLAGRARAAGSEPTPAMAEVARDCGYLPFALALAGAMIASTSWERLLERLQAADL
                     RFMEARIPGYPDTDLLRTLQVSVDHLRESEGERGRTAVDRYLEIAAFRWETGVPEAAL
                     AILWRARGGLDECAAEDVLTRLARCGLVRAEGHAPRRLVRLHDLQRDYLWCHVERGAR
                     AREHAAIVDAYASFAAGAPFVAHDDGYFLENCFHHLEHADRAGEMHAILAREAGDGAS
                     AWWATRFAHGQVLGFVADVRRASVHAWRSVSVGQRPDAEAVARAVRYALMIASARSAV
                     GEVPLQLVSRLLAEKLWTADQALALIEWAGHGSGRIDALGAVFPYLPPDRRPQAVADA
                     LQLARGQVEAGDRTRRLLQLVPLLEGASRLQVLEEALGRPDEASIDLLCQVVRLAPDR
                     LDLLDRAIQAARDSREAFSRKGAIEQLAGVVPSSRLDELLPLVDGAGSDEHQAEVLTV
                     LAERLSATRRGELAARAFALLSHGEDFKGAGVTAVKERARVVRLLPPEQQAAAATGVM
                     TELERLKSDRHRLVAIADVVPFLTGLERTRAELRACELAGHVDAFHIAEVVEDGGRGR
                     WLAAAAPEFCQPLLTALVEAARNAETSSRRIAAIAALLPCLDPARARAVLIEEERHAR
                     LIGDVEQRVTCIGALTRTLPPAAREPVVSRMLAAIAGGTGGRWSARSLAALAPVAAAG
                     QLARMAAALSTVPGLERARAAAALFAATARLGRWDRALGFLPELDNAHHLRLALDQLP
                     EEVPADVVRTIIRGAADAKDAELGATAIAPLARRLPEDERETACEMGLRAAEVLRLPD
                     RYRLLEALIPAIPCER"
BASE COUNT          410 a         1033 c          986 g          364 t
ORIGIN      
        1 gccgctcgca cgggattgcc gggatcagcg cttccaggag ccgatagcga tcgggaagcc
       61 gcagcacctc cgctgcccgc agtcccatct cgcaggcggt ctcgcgctcg tcctccggga
      121 gccggcgggc aagtggagcg atcgcggttg caccgagctc ggcgtctttc gcgtcggccg
      181 cgccccggat gatcgtgcgc accacatccg cgggcacctc ctcagggagc tggtcgagcg
      241 cgagcctcag gtggtgcgcg ttgtcgagct ccggcaggaa acccagcgcc cgatcccacc
      301 gtcccaggcg tgcggtcgcg gcgaacagcg ccgcggcagc cctggctcgc tccaggcccg
      361 ggactgtcga cagcgcggcc gccatccgag cgagctgccc ggccgcggcg accggcgcga
      421 gcgcggccag cgagcgagct gaccaccggc cgccggttcc gccggcgatg gcggcaagca
      481 tccggctcac gaccggttca cgcgccgcgg ggggcagcgt cctcgtcaga gcgccgatgc
      541 aggtcacgcg ttgctcgaca tcgccgatca gccgcgcgtg ccgttcttcc tcgatcagga
      601 ccgctcgcgc tcgcgccggg tcgaggcagg ggagcagtgc ggcgatcgcc gcgattcgac
      661 gggacgatgt ctcggcgttc ctcgccgcct cgaccagcgc cgtcaggagc ggctggcaga
      721 actcgggcgc cgctgccgcg agccaccgcc cgcggccacc gtcctcgacg acctccgcga
      781 tgtggaacgc gtcgacgtgc ccggcgagct cgcaagcacg cagttccgcg cgcgtgcgct
      841 ccaggccggt gaggaacggc acgacatcgg cgatcgctac caggcggtgg cgatccgatt
      901 tgagccgctc gagctcggtc atcacccccg ttgctgccgc cgcctgctgc tccggcggca
      961 gcaggcgaac gacgcgcgcg cgctccttga ccgctgtcac gccggcgccc ttgaagtcct
     1021 cgccgtgcga cagcagcgcg aacgctcgtg cggcgagctc tccgcggcgt gtggcggaca
     1081 gccgctcggc gagcacggtg agcacctccg cctggtgctc gtcggagccg gcgccgtcga
     1141 cgagcggcag gagctcgtcg aggcggctgg acggcacgac gccggcgagc tgctcgatgg
     1201 cacccttccg cgagaatgct tcacggctgt cgcgcgccgc ctggatggcg cggtcgagca
     1261 ggtcgagccg atcgggtgcg agacggacga cctgacacag caagtcgatc gatgcctcgt
     1321 cgggacgccc cagcgcttcc tcgaggacct gcaagcggct ggcgccctcg aggagcggga
     1381 ccagctgcag cagtcgccgg gtgcggtctc ccgcctcgac ctgaccgcgt gccagctgca
     1441 gcgcgtccgc gacggcctgt ggcctgcggt ccggcgggag atacgggaac accgcgccga
     1501 gggcgtcgat ccgccccgag ccgtgccccg cccattcgat gagggccagc gcctgatcgg
     1561 cggtccacag cttctcggcg agcagccggc tcacgagctg gagcggcacc tcccccacgg
     1621 cggagcgggc cgacgcgatc atgagcgcgt agcggacagc gcgtgccacg gcctccgcgt
     1681 ccggacgctg tccgacggag acgctgcgcc aggcgtgaac ggaggcacgg cggacgtccg
     1741 cgacgaagcc gagcacctgc ccgtgcgcga agcgggttgc ccaccacgca ctggcgccgt
     1801 cgccggcttc gcgagcgagg atcgcgtgca tctcgccggc gcggtctgcg tgctcgagat
     1861 ggtggaagca gttctcgagg aaataaccat catcgtgcgc cacgaacggc gcgcccgccg
     1921 caaagctcgc ataggcgtcg acaatcgctg cgtgctctct cgcgcgcgcc ccgcgctcca
     1981 cgtggcacca gaggtagtcc cgctgcagat cgtggagacg gacgagacga cgaggcgcat
     2041 gcccctcggc ccggacgagg ccacatcgcg caaggcgcgt cagcacatcc tctgcggcac
     2101 attcgtcgag gccgccgcgt gcccgccaca ggatcgcgag cgcagcctcg ggaacaccgg
     2161 tctcccaccg aaatgcggcg atctcgagat agcgatccac ggcggtccgt ccgcgctcgc
     2221 cttccgattc gcgaagatgg tccacgctca cctgcagcgt acgcagcagg tccgtgtcgg
     2281 ggtagccggg gatcctcgct tccatgaagc gcaggtcggc cgcctgtaac cgctccagca
     2341 gccgctccca cgacgtgctc gcgatcatcg cgcccgcgag cgcgagcgcg aacgggagat
     2401 acccgcagtc gcgagcgacc tccgccatgg ccggcgtcgg ctcggatccg gctgcacgcg
     2461 cgcggccggc gaggtacgca agcgcctcgt cggggctgaa cggccccagg ctcacggtgg
     2521 gcgcgccgac ctcgaggacg gaaggctcgc gcgcagtgag cagcaggcgg cagcgtacgc
     2581 cgagcgcttc gcgcaccagt ttgaccggat cccggctcca gatgttgtcg agcacgatca
     2641 ggacggcggc gcggtcgaga cgcacggcca gttgctggcg cgcggccgcc tccgtggcgt
     2701 acttcgcgag atcgtccccg aggcgcagcg cgatctgctg caacaccgcg agcggctgcg
     2761 cgtccctccc cacagcgacc cagatcacgc cgt
//
LOCUS       DASVOR010001976         1508 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_282841,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001976 DASVOR010000000
VERSION     DASVOR010001976.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282841"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VF516_36695"
     CDS             <1..628
                     /locus_tag="VF516_36695"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8113344.1"
                     /translation="RPASPAGPIDGRYKGREIAATCSYLGADWLDRPEREQREQPERV
                     LDALKLDPHATVADVGAGTGYFTVRLARRVAHVHATDLQPEMLQLLGDRLAREHLTNV
                     ELHLATGHDAALPARCCDLILLVDVYHELSDPPGVMAGIRRALTEHGRLVLVEYRGED
                     PAVPIKPEHKMTLRQIQQELTQLGFHFVESLEFLPDQRVVVFDAKPGP"
     gene            complement(634..>1508)
                     /locus_tag="VF516_36700"
     CDS             complement(634..>1508)
                     /locus_tag="VF516_36700"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8113345.1"
                     /translation="AGRMPDAPPPGVGELGTAVAAQLHQRLSRLGKKLPTPFDLDDLI
                     VDDDTRAALVEIAAAASQRRSIRDAFKLRGAQGISVLFSGHPGVGKTMSGTVLARRLG
                     LDIYEVDLSQVVSKWLGETEKNLSDVFDAAEPGHVVLLFNEADSLFGKRTTDVKSSND
                     RYANLETNYLLQRLERFGGLAILTTNLTSAIDQAFKRRFTYDVFFSFPSPDMRAELWR
                     RTLPERARGAGIDFDALAERYELSGGFIKVACERAAYVAGAGKTEITDDMLRQTIERM
                     YRERGKLSSVGPLE"
BASE COUNT          209 a          526 c          557 g          216 t
ORIGIN      
        1 ccgccccgcc agcccggccg gaccgatcga cggccgctac aagggccggg agatcgccgc
       61 gacgtgcagc tacctcggcg ccgactggct cgatcggccc gagcgcgagc agcgcgagca
      121 gcccgagcgc gtgctcgacg cgctgaagct cgatccgcac gccaccgtcg ccgacgttgg
      181 cgcgggcacc ggctacttca ccgtgcgcct cgcgcgccgg gtcgctcacg tccacgccac
      241 cgacctccag cccgagatgc tgcagctgct cggcgaccgg ctcgcccgcg agcacctgac
      301 caacgtcgag ctccacctcg ccaccggcca cgatgccgcg ctgccggcgc gctgctgcga
      361 cctgatcctc ctggtcgacg tctaccacga gctgtccgac ccgcccggcg tcatggccgg
      421 catccgccgc gcgctgaccg agcacggccg gctggtgctc gtcgagtacc gcggcgagga
      481 ccccgccgtc ccgatcaagc ccgagcacaa gatgaccctg cgccagatcc agcaggagct
      541 cacccagctc ggcttccact tcgtcgagtc gctcgagttc ctgcccgatc agcgcgtcgt
      601 cgtcttcgac gcgaagcccg gtccgtagcg cggctactcc agcgggccga ccgagctgag
      661 cttgccgcgc tcgcggtaca tgcgctcgat ggtctggcgc agcatgtcat cggtgatctc
      721 ggtcttgccg gcgccggcga cgtaggcggc gcgctcgcag gcgaccttga tgaagccgcc
      781 ggacagctcg tagcgctcgg cgagggcgtc gaagtcgatg ccggcgccgc gggcgcgctc
      841 ggggagggtg cggcgccaca gctcggcgcg catgtcgggg ctggggaagc tgaagaacac
      901 gtcgtaggtg aaccggcgct tgaaggcctg gtcgatcgcc gaggtcaggt tggtggtgag
      961 gatggcgagg ccgccgaacc gctcgaggcg ctgcagcagg tagttggtct cgaggttggc
     1021 gtagcggtcg ttgctgctct tgacgtcggt ggtgcgcttg ccgaacagcg agtcggcctc
     1081 gttgaacagg aggacgacgt ggccgggctc ggcggcgtcg aacacgtcgg acaggttctt
     1141 ctcggtctcg cccagccact tggacacgac ctgggacagg tcgacctcgt agatgtcgag
     1201 gccgaggcgg cgggcgagca cggtgccgga catggtcttg ccgacgccgg ggtggccgga
     1261 gaacaggacc gagatgccct gcgcgccgcg cagcttgaag gcgtcgcgga tcgagcggcg
     1321 ctggctggcg gcggcggcga tctcgaccag cgcggcgcgg gtgtcgtcgt cgacgatcag
     1381 gtcgtcgagg tcgaacgggg tcggcagctt cttgccgagc cgcgacagcc gctggtggag
     1441 ctgcgcggcg accgcggtgc cgagctcgcc gacgccgggc ggcggggcgt cgggcatccg
     1501 gccggcgg
//
LOCUS       DASVOR010001977         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_400074,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001977 DASVOR010000000
VERSION     DASVOR010001977.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400074"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="VF516_36705"
     CDS             complement(<1..402)
                     /locus_tag="VF516_36705"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8113346.1"
                     /translation="MSNCPRVTLVVVFVASLTGCADNLAPHTAPETPTVGNDRATVTG
                     HVLDTSGAPVAGAMVAVRTSGEHAMVDATGAFTLDVPANTTLTLSATAPNMAPTLLQQ
                     FLVAPGASGTVEIPLVTSDHFKSLVGMGGNAN"
     gene            complement(1008..>2256)
                     /gene="rodA"
                     /locus_tag="VF516_36710"
     CDS             complement(1008..>2256)
                     /gene="rodA"
                     /locus_tag="VF516_36710"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEX8113347.1"
                     /translation="WHPGQRAFVEHLKTQDKASGRPYSLRYSGAMVADVHRTLLPRTT
                     IGVSRWRRFRAAVDWPLIVVIVALCTLGLLNLYSATRGVRHHAKFDTQVQWMVVGAIG
                     FVVATIVDYRTMVRLAWSGLGAAIALLLVARVLGKPPAGDTNHGITYRWLNIGGMGIQ
                     PSELAKLMVILVLARVFQDPEPESYTPRRLLGRLAILVIPIGLIAVQPDLGTAVLLGL
                     IVLSVGFLAMPSVWPMVYVTLAGLLAIPVLWESMHDYQQKRILGFIDCGIAPTKECWH
                     TQQSIYAVGSGKIWGKGFLEGTQNQFNFVPEHWTDFPYSVWAEEWGFAGSVVLLAIFG
                     FLLVWILNVALSARDRIGAVICLGVAAMTFWHVVVNVAMVLGLAPVVGVTLPFISYGG
                     SSLVTFFLAMGLVANVSLRKHGY"
BASE COUNT          433 a          702 c          766 g          355 t
ORIGIN      
        1 attcgcgttg ccccccatcc cgaccaggct cttgaagtgg tcgctggtga ccagcgggat
       61 ctccaccgtc ccgctggcgc ccggcgcgac caggaactgc tgcagcagcg tgggcgccat
      121 gttcggcgcg gtcgccgaca gcgtcagcgt ggtgttggcc gggacgtcga gcgtgaacgc
      181 gccggtggcg tccaccatgg cgtgctcccc ggaggtccgc acggccacca tggctcccgc
      241 cacgggggcg ccgctggtgt ccaggacatg gccggtgacg gtcgctcggt cgtttccgac
      301 cgtaggggtt tcgggggcag tgtgaggtgc caggttgtcc gcacaaccag taagcgaggc
      361 cacaaacact acaaccaatg tgactcgtgg acagttactc atcgaaaaat cctgtgtgct
      421 ggatgactat gacgtcatac taggaaaatt tcgcctccac tgtcaacgtc aacttactgg
      481 tgaccacatg agtctccagg tgatttgagt cacaaaacca gttcgcaatc ggtatcaatc
      541 taaggaaaga tgaatagtta gggactaacc acccatgtaa gaagatcttc cgtctgactg
      601 aaaaaaagcg cgaaaaacac ctcttcgggt gctgatggca ggctcgccag gatttcacag
      661 ccgcgattcc cggccgctac cgcatcacga gctttcaata tcttacgtga tctgcagctc
      721 gaacagcctc tgcatgaacc gcacggccgc ttacagcgct ccgtcacctc actctcgact
      781 agcgatgctg cagttccata tatgagatcg gggaacggtg ctcgaccgca tctaccgtcc
      841 gctcggatgg tacgagcctc acgatcacgc tgccctgatc cgctgcgggt ccgacgggcg
      901 atccatgcga ggttctcgca gatcgtcccg catgtgcgac accgtcagcc ggcggcgggc
      961 ttgtcggctt gcccagctcg ctgtggacct cccacccgag atcggcgtca gtagccgtgc
     1021 ttgcgcagcg agacgttggc gaccaggccc atcgcgagga agaaggtgac cagcgagctg
     1081 ccgccgtagg agatgaacgg cagggtgacg ccgacgaccg gggcgaggcc gagcaccatc
     1141 gcgacgttga ccacgacgtg ccagaacgtc atcgcggcga cgccgaggca gatcaccgcg
     1201 ccgatgcgat cgcgcgccga cagggcgacg ttgaggatcc agaccaggag gaagccgaag
     1261 atcgcgagca ggacgaccga gccggcgaag ccccactcct cggcccagac cgagtacggg
     1321 aagtcggtcc agtgctcggg gacgaagttg aactggttct gggtgccctc gaggaagccc
     1381 ttgccccaga tcttgccgga gccgacggcg tagatcgact gctgggtgtg ccagcactcc
     1441 ttggtcggcg cgatgccgca gtcgatgaag ccgaggatgc gcttctgctg gtagtcgtgc
     1501 atcgactccc acagcaccgg gatggcgagc aggccggcga gcgtgacgta gaccatcggc
     1561 cacaccgacg gcatcgcgag gaagccgacc gacaggacga tcaggccgag caggacggca
     1621 gtgccgagat cgggctggac ggcgatcagg ccgatcggga tcacgaggat ggcgagccgg
     1681 ccgagcagcc ggcgcggcgt gtagctctcg ggctcggggt cctggaacac gcgggccagg
     1741 acgaggatga ccatgagctt ggcgagctcg gagggctgga tgcccatgcc gccgatgttg
     1801 agccagcggt aggtgatgcc gtggttggtg tcgccggcgg ggggcttgcc gagcacgcgg
     1861 gcgacgagca ggagggcgat cgccgcgccg aggccggacc acgcgaggcg gaccatggtg
     1921 cggtagtcga cgatggtggc gaccacgaag ccgatcgcgc cgacgaccat ccactggacc
     1981 tgggtgtcga acttggcgtg atgacggacg ccgcgggtcg ccgagtacag gttgagcaga
     2041 ccgagcgtgc acagcgcgac gatgacgacg atcagcggcc agtcgaccgc ggcgcggaac
     2101 cgccgccacc ggctgacgcc gatcgtcgtg cggggcagga gcgtccggtg cacgtcggcc
     2161 accatcgcgc cggaatagcg cagcgaatag ggccggccgc tcgccttgtc ctgcgtcttc
     2221 aagtgctcga cgaaggcgcg ctgacccggg tgccac
//
LOCUS       DASVOR010001978         2283 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488576,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001978 DASVOR010000000
VERSION     DASVOR010001978.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2283
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488576"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2283
                     /locus_tag="VF516_36715"
     CDS             <1..>2283
                     /locus_tag="VF516_36715"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8113348.1"
                     /translation="TGALYVPVAVTHMQFHRRVGAAPVVCHVVLGERTGDEIAAEIRL
                     RDEQGTIAEVGFRGKRVDRERFVRGAAAQRDLFYRTDWQRVAAPAHPARSPGRYLVLA
                     DRGDVATRLTALLGARGATCTLVTAAVLRDAAAVRRTVDDALRGDPPLVGIVHLGSLD
                     APPYATTSLAALDAARTTGCDSILHVVQAIVHAAPRHAPRLHLVTAGAQAVGDASSVS
                     PAQTAVWGLARVIAHEHPELRCACADLSLVPSETEIAALADEIAADDREDQVAFRDDA
                     RHVARLVPYRLGARTAPAKQPAATVPAGDQPYRLEIDAPGVLEDLVLRAVPRPAPRAD
                     EVEIEVRAAGINFVDVLSALGIRPDHTDGRTQLGGECSGIVTRIGERVTDVKPGDAVI
                     AALVPDAFSSFVRVPSRFVVAKPAHIGFEDAATLPITFMTAYWATHHKAHLERGERIL
                     IHAAAGGVGLAAVQLARAVGAEIFATAGSEGKRAYLRGLGIEHVMHSRSLRFADEIRA
                     RTGGEGVDVVLNCLTGDALIQSLGVLRAHGRFLEIGKRDIYDDHKLGLYPFRQNLSYF
                     AIDLIRMFGDRPERFAELLHEVVGLVEGGTIAPLPWRAFPISQAQDAFRHIARAQHIG
                     KVVLTFPDPGARLSPAAATASGRFRTDASYLITGGLGGLGLAVAEWMVEHGARHLALV
                     GRRGPQGAALDAIARLERVGARVLALDADVSQPAEVDPLIATIGRELPPLRGVVHAAG
                     VLDDGVLLNQTAERFAAVMAP"
BASE COUNT          294 a          849 c          811 g          329 t
ORIGIN      
        1 accggcgcgc tctacgtgcc ggtggcggtc acgcacatgc agtttcatcg ccgggtcggc
       61 gcggcacccg tggtgtgcca cgtcgtgctc ggcgagcgca ccggcgacga gatcgccgcc
      121 gagatccgcc tgcgcgacga gcagggcacg atcgccgagg tcggctttcg cggcaagcgc
      181 gtcgatcgcg agcggttcgt gcgcggggct gcggcccagc gcgacctgtt ctaccggacg
      241 gactggcagc gcgtcgcggc cccggcgcac ccagcgcgct cgccggggcg gtacctcgtg
      301 ctggccgatc gcggcgacgt ggccacccgg ctcaccgcgc tgctcggtgc acgtggagcc
      361 acctgtaccc tggtgactgc tgcggtgctt cgcgacgcag cggcggttcg ccgcactgtg
      421 gacgacgcgc tccgcggtga cccgccgctc gtgggcatcg tgcacctcgg cagcctcgat
      481 gcaccgccgt atgcgaccac cagcctcgcg gcgctcgatg ccgcacggac gaccggctgc
      541 gactcgatcc tgcacgtcgt ccaggcgatc gtccacgcgg cgccgcgcca tgctccgcgg
      601 ctgcacctcg tcaccgcggg agcccaggcg gtcggggacg catcgtcggt gtcgcctgcg
      661 cagaccgcgg tgtggggcct cgcgcgcgtg atcgcgcacg agcatcccga gctgcgctgc
      721 gcgtgcgccg acctcagcct cgtcccatcg gagaccgaga tcgccgcgct ggccgacgag
      781 atcgccgccg acgatcgcga ggatcaggtc gcgttccggg acgatgcgcg tcacgtcgcg
      841 cgcctcgtcc cgtaccggct cggtgcgaga acagcgcccg cgaagcaacc cgcggccacg
      901 gtcccagctg gcgaccagcc gtaccggctg gagatcgatg cgcccggcgt gctcgaagat
      961 ctggtgttgc gcgccgttcc gcgcccggcc ccgcgggcgg acgaggtcga gatcgaggtc
     1021 cgcgccgccg ggatcaactt cgtcgacgtg ctgtcggcgc tcggcatccg gcccgatcac
     1081 accgacggca ggacccagct cggcggcgag tgctccggca tcgtcacccg gatcggcgag
     1141 cgcgtcaccg acgtcaagcc cggggacgcg gtgatcgccg cgctcgtgcc ggacgccttc
     1201 tcgtcgttcg tccgggtgcc gagccggttc gtcgtggcca agcccgcgca catcggcttc
     1261 gaggacgccg cgacgctccc gatcacgttc atgaccgcgt actgggcgac gcaccacaag
     1321 gcacacctcg agcgcggcga gcgcatcctc atccacgccg ccgccggcgg tgtcgggctc
     1381 gcagcggtgc aactcgcgcg cgcggtgggc gcggagatct tcgcgaccgc cggcagcgag
     1441 ggcaagcgcg cgtacctgcg tgggctcggg atcgagcacg tgatgcattc gcgctcgctc
     1501 cgcttcgccg acgagattcg cgcgcgtaca ggcggcgaag gcgtggacgt ggtcctgaac
     1561 tgcctgaccg gcgacgcgct gatccagagc ctcggcgtgc tccgcgcgca cggccgcttc
     1621 ctcgagatcg gcaagcgcga catctacgat gaccacaagc tcgggctcta cccgttccgc
     1681 cagaacctgt cgtacttcgc gatcgatctg atccggatgt tcggcgaccg gccggagcgg
     1741 ttcgccgagc tcctgcacga ggtcgtgggg ctggtcgagg gcggcacgat cgcgccgctg
     1801 ccgtggcggg cgtttccgat ctcgcaggcg caggacgcgt ttcgccacat cgcccgcgct
     1861 cagcacatcg gcaaggtcgt gctgacgttc ccggatcccg gggcgaggtt gtcaccggcc
     1921 gcagcgacgg ccagcgggcg gttccgcacc gatgcgagct acctcatcac cggcggcctg
     1981 ggcggcctgg ggctcgcggt cgcggaatgg atggtcgagc acggggcccg ccacctcgcg
     2041 ctcgtcggcc gccgtggacc gcagggcgcc gcgctcgatg cgatcgccag gctggagcga
     2101 gtcggtgcgc gcgtcctcgc gctcgacgcc gacgtctcac aaccggccga ggtcgacccg
     2161 ctgatcgcca ccatcggccg cgagctgcca ccgctgcgcg gtgtcgtcca tgccgccggt
     2221 gtcctcgacg acggcgtcct cctcaaccag accgccgagc ggttcgccgc cgtgatggcg
     2281 ccc
//
LOCUS       DASVOR010001979         2261 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_313517,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001979 DASVOR010000000
VERSION     DASVOR010001979.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313517"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VF516_36720"
     CDS             complement(<1..1127)
                     /locus_tag="VF516_36720"
                     /inference="COORDINATES: protein motif:HMM:NF017945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEX8113349.1"
                     /translation="MRLNELLDVVFRWAHLIAGIMWIGNSMLFNWLDRNLEKHGPLAP
                     LSQGKIYMVHSGAFYDVEKKLLEPGELPDRLHWFKWQNGFTWMTGIALLVIVYYANGA
                     AFLIDVNVQQLDPLVAIVASASSLFLGWILYDGLWNAIGEHRPRLATALSLAMLVAAI
                     IGYTQMFAGRAAYILTGVLIGTIMTGNVWLCILPSQRALIAATRAGQPQDPGLSLRAK
                     QRSIHNNYLTFPLLFMMLSNHFPSAYGHHLNWLVLIAVMVGGAGVRHFMNVRYRGEGR
                     QMIPIAWLTPAAIMAGIAIAGLLVITRIPAAPEVDVTYAVGFARAQEIIVKRCVPCHS
                     SHPTDPAFPAPPNNVVFETADQIKLMVPRIRERAVESKTMP"
     gene            complement(1204..2013)
                     /locus_tag="VF516_36725"
     CDS             complement(1204..2013)
                     /locus_tag="VF516_36725"
                     /inference="COORDINATES: protein motif:HMM:NF019887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HEX8113350.1"
                     /translation="MYRLLALDLDGTLLRRDGVVDDRDIAAIAELQAAGVVVTIVTGR
                     LHSGATFAARSCRIDGAIGCVDGSHLVELTSGRTLVHHGMAGDLAAVVRDAMARHELS
                     AFVFDAAGIHHEHAGAPYAQYISTWSPNLRIVEQDVAWQTEPLAAVAIGEPLAVAAAH
                     AALREHAARLYSVRFEIWSHPGKYALMVRAAGVTKGTALAELCRRAGISLAEAVAVGD
                     WINDVPMFEVAGRSFAMGTAPDDVRAKATDVLSATAFDGGGIVEAIRRAWG"
BASE COUNT          343 a          774 c          832 g          312 t
ORIGIN      
        1 aacggcatgg tcttgctctc gaccgcgcgc tcgcggatcc gcggcaccat cagcttgatc
       61 tggtcggcgg tctcgaacac gacgttgttg ggcggcgccg ggaacgccgg atccgtcggg
      121 tggctgctgt ggcagggcac gcagcgcttg acgatgatct cctgggcgcg cgcgaagccg
      181 acggcgtagg tgacatcgac ctcgggagcg gcggggatcc gcgtgatgac gagcaagccg
      241 gcgatcgcga tcccggccat gatcgcggcc ggggtgagcc aggcgatcgg gatcatctgg
      301 cggccctcgc cgcggtagcg gacgttcatg aagtggcgca cgccggcgcc gccgaccatc
      361 accgcgatca gcaccagcca gttgaggtgg tggccgtagg cgctggggaa gtggttcgac
      421 agcatcatga acagcagcgg aaacgtcagg tagttgttgt ggatgctgcg ctgcttggcg
      481 cgcagcgaca gccccgggtc ctgcggctgg ccggcccggg tcgcggcgat cagggcgcgc
      541 tgcgacggca ggatgcacag ccagacgttg ccggtcatga tcgtgccgat caggacgccg
      601 gtcaggatgt aggcggcgcg gccggcgaac atctgggtgt agccgatgat cgccgcgacc
      661 agcatcgcga gcgagagcgc cgtggccagg cgcgggcggt gctcgccgat cgcattccac
      721 agcccgtcgt agaggatcca gcccaggaac agcgagctgg cggacgccac gatggcgacc
      781 agcggatcga gctgctggac gttgacgtcg atcaggaacg cggcgccgtt ggcgtaatag
      841 acgatgacca gcagcgcgat gccggtcatc caggtgaagc cgttctgcca cttgaaccag
      901 tgcaggcggt ccggcagctc gccgggctcg agcagcttct tctcgacgtc gtagaacgcg
      961 ccggagtgga ccatgtagat cttgccctgc gacagcggcg cgagcgggcc gtgcttctcc
     1021 aggttgcgat cgagccagtt gaacagcatc gagttgccga tccacatgat cccggcgatc
     1081 aggtgggccc agcgaaacac gacgtcgagc agctcgttca ggcgcacgag gccgactata
     1141 cgcagacctc gcccggcgat tcacgctccg ccgctcgccc tggtcgtccg cgacgcggtg
     1201 tgctcagccc caggcccgcc ggatcgcctc gacgatgccg ccgccgtcga acgccgtggc
     1261 ggacagcaca tcggtcgcct tggcgcgcac gtcgtcgggc gcggtgccca tcgcgaacga
     1321 ccggccggcg acctcgaaca tcggcacgtc attgatccag tcgccgaccg cgaccgcctc
     1381 ggcgagcgag atcccggcgc gccggcacag ctcggccagc gcggtgccct tggtgacccc
     1441 ggcggcgcgc accatcagcg cgtacttgcc ggggtgcgac cagatctcga accggacgct
     1501 gtacagccgg gcggcgtgct cgcgcagcgc ggcgtgggcg gcggcgaccg cgagcggctc
     1561 gccgatcgcg accgcggcga gcggctcggt ctgccaggcg acgtcctgct cgacgatccg
     1621 caggttcggc gaccacgtgc tgatgtactg ggcgtacggg gcgccggcgt gctcgtggtg
     1681 gatgccggcg gcgtcgaaca cgaacgcgga cagctcgtgg cgcgccatcg cgtcgcggac
     1741 caccgccgcg aggtcgccgg ccatgccgtg gtgcacgagg gtgcggcccg aggtcagctc
     1801 gaccaggtgg ctgccgtcga cgcagccgat cgcgccgtcg atccggcagg accgcgccgc
     1861 aaaggtggcg ccggagtgca gccggccggt gacgatggtc acgacgacgc cggcggcctg
     1921 gagctcggcg atcgcggcga tgtcgcggtc atcgacgacg ccgtcgcggc gcagcagcgt
     1981 gccgtcgagg tcgagcgcga gcaaccggta catgagagcc cgccactgta gcacccggcc
     2041 ctgacctcgc ccgccgtccg ccgcagcgcg tcatcgcacc cggtcagcct ggatctcact
     2101 cgcccggccc gtccggccgg accggcgcga tctcggcgac ggcggcggcg gcgagcgcga
     2161 cgtgcaccgc gccgatgttc tcgtcgcggt gcgcgccctt gctggtgccc gggatcggca
     2221 gcatcaccgg cgagcgcgcg agcagccagg cgagcgccag c
//
LOCUS       DASVOR010001980         3859 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488593,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001980 DASVOR010000000
VERSION     DASVOR010001980.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3859
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488593"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..407
                     /locus_tag="VF516_36730"
     CDS             <1..407
                     /locus_tag="VF516_36730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113351.1"
                     /translation="ERAPRADAAPPARSKDEQVCYDRGYAKGLDSTAYDRGLADGKLA
                     NPKYQDGYHATYPAAYSSGQTAGLTDGYSAGHHDGYSDGYDAGFSVTYDGCYGGAYSD
                     AYDSTYSDGADAGYSSGYSDGYDSGYSDGSDC"
     gene            855..1019
                     /locus_tag="VF516_36735"
     CDS             855..1019
                     /locus_tag="VF516_36735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113352.1"
                     /translation="MQLGTILERQTTASLQRGVRAGGGRRQAQLQANAELAKLLGAQR
                     FARYEAARPS"
     gene            1162..1413
                     /locus_tag="VF516_36740"
     CDS             1162..1413
                     /locus_tag="VF516_36740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113353.1"
                     /translation="MLVAVIGMLAASAVAHAGTRRIAVVVGPNTGNDDRTPLHFAELD
                     AGKFARVLGELGGGEPADLAGQPGLDLAARGERDRDGAR"
     gene            1438..2712
                     /locus_tag="VF516_36745"
     CDS             1438..2712
                     /locus_tag="VF516_36745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113354.1"
                     /translation="MTPRAGGPSNLAELEVLAGHRARSLTMKHLLAYMLVVGVGCGTG
                     VHSNPGVPPDGPPVMVPDGPLPDAPLETQRLGMNDISIVASTIFPDVSRRITGIGHTR
                     DVVPRALYARLATSHGDIIDDFDKFMVFGIRFDLCDRLVTGPCPEGVDGSLRLVFQPL
                     GFPISATDVGLHAFYTIPAAELGYVINELRAVARLEGTGPMIDGGLTGLHNLSTEGEM
                     RLDALLDRYAIADRLIRLDLMGQDARSSVPHVVFRGIELHDGQWNDIPVADVDATQQD
                     AALTGSDPSYDVTPVADRPQNLALTLTSDAFNAAAPAAQRDALDALVATQNPQLHTSS
                     GGSSTVQCVACHVATYLAVHRAQVAAIALDSLPSRFQTTHDARITDGISTTTPGSLHN
                     FGWLGPNVAISQRAANETAMVLDEIQQRFPVP"
     gene            2768..3205
                     /locus_tag="VF516_36750"
     CDS             2768..3205
                     /locus_tag="VF516_36750"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HEX8113355.1"
                     /translation="MVRPGAAAAAALDPCHFTSFIGYEWTNNCTGDGGSAATCHKNVI
                     FGDTNVPVAPFDSLSNPTQESLWSALDHGCNGGRGSPCGCTRPGTRPPTSAPTRASRR
                     RSSHQSSRLGSDGSRVASTARARPPASTVTTSTAWRSSTSRLG"
     gene            complement(3250..3645)
                     /locus_tag="VF516_36755"
     CDS             complement(3250..3645)
                     /locus_tag="VF516_36755"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEX8113356.1"
                     /translation="MAERFAIPIHPTPEDIDELGHVSNLVYLRWVAEVAMAHSRARGW
                     DMQAYRALGAVWMVRRHEIDYIAQVTLGQELRAETWVDSWRAASCIRKTELVRDGTVV
                     ARAATTWAMIGLASGRPQRIPEELLAAFR"
     gene            3668..>3859
                     /locus_tag="VF516_36760"
     CDS             3668..>3859
                     /locus_tag="VF516_36760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113357.1"
                     /translation="MSDETTVRALRGRLEHGQATAAEAVATLAVWAVEAPTRATRLDA
                     CQLLGDLAGRALGAEWELAE"
BASE COUNT          590 a         1337 c         1360 g          572 t
ORIGIN      
        1 ccgagcgcgc gccccgggcc gacgcggccc cgccggcgag gtcgaaggac gagcaggtgt
       61 gctacgaccg cggctacgcg aagggactcg acagcaccgc ctacgatcgc ggcctcgccg
      121 acggcaagct cgcgaacccc aagtaccagg acggctacca cgcgacctac ccggccgcgt
      181 actcgagcgg ccagaccgcc ggcctcaccg acggatacag cgcgggccac cacgacggct
      241 acagcgacgg ctacgacgct ggcttctcgg tcacctatga cggctgctac ggcggcgcgt
      301 actccgacgc gtacgacagc acgtacagcg acggcgcgga tgccggctac tcgtcgggct
      361 acagcgacgg ctacgatagc ggctactccg atggcagcga ctgctagcca gacgatgccc
      421 cggcttcgcc ggggtggccc gtgcggcgac ggtggtcagt tcgccgtgct cgccggcgcc
      481 ggatccgcgg cgggttcggt cttggccgcg ggcggagcgg ccggctggtt gtcgttctgt
      541 gcggcctgat ccagggcggt gggatagcta ctgcgcggac acccggggac ccgggacacg
      601 gggtacatgc gtgaatgtgg ggccgtcttc gaccggttgc tcgtgctcga cgcctcccgt
      661 ctgcacggtg tcgtgtccga cgaactgtgg gcagccggcc accgtcgggc ctggctgccc
      721 gccaacgaac tgtcccgaca cggatacctg taccgaggcc acgtgtggcc agaccgtgtg
      781 cggtcaacaa tgccccgcct gcggatgcaa cggcaccgtg aacgcatgcg gtgagtatga
      841 tcccggcgga gcgcgtgcag ctcggcacga ttctcgagcg ccagaccacg gcctcgctgc
      901 agcgcggggt cagggccggc ggcggacgcc gccaggcgca attgcaggcc aacgccgagc
      961 tcgcgaagct ccttggtgcc cagcgcttcg cacgctacga agcggcccgt ccttcatgag
     1021 gatacatcgg cacctccaca gcgcgccgga tcgcagcatg cgcttgcctg gatttaccgg
     1081 accgcagcga acgactggct cgacgagatc cgcgatcgta gcgcgggtga gccaagcatg
     1141 atggtcggag gagcgatccg cgtgctggtc gccgtgatcg gtatgcttgc cgcgagcgcg
     1201 gtcgcgcatg ccggcacccg acggattgcc gtggtcgtcg gccccaacac cgggaatgat
     1261 gaccggacgc cgctgcactt cgcggagctc gacgccggca agttcgcgcg ggtgctcggc
     1321 gaactcggcg gcggcgagcc ggccgatctg gctgggcaac caggtctcga tctcgcagcg
     1381 cgtggcgaac gagaccgcga tggtgctcga tgagatccag cagcgctttc ccgtcccgtg
     1441 acgccgcgag cgggcggacc tagtaacctt gcggagctcg aggtcctggc gggtcatcga
     1501 gcaaggagtc tcacaatgaa gcacttgctc gcgtacatgt tggtcgtcgg tgtcggctgt
     1561 ggcacgggtg tacacagcaa tcccggtgtg ccgccggatg gcccaccggt catggttccg
     1621 gatggcccgc tgcccgacgc gccgctcgag acccagcgcc tgggcatgaa cgacatctcg
     1681 atcgtggctt cgacgatatt cccggacgtg tcgcggagaa tcaccggcat cggccacacg
     1741 agagacgtcg taccgcgtgc gctctatgcg cggcttgcga catcgcatgg cgatatcatc
     1801 gatgacttcg acaaattcat ggtcttcggg atccgcttcg acctgtgcga tcgcctggtg
     1861 accggcccgt gcccggaagg agtcgacggt agcctgcgac tggtgttcca gccgctgggc
     1921 ttcccgatct ccgcgaccga cgttggcctg cacgccttct atacgatccc cgccgcggag
     1981 cttggatacg tgatcaacga gctgcgtgcc gttgcgcgcc tcgaggggac ggggccgatg
     2041 atcgatggcg ggctgaccgg gttacacaac ctgtctaccg agggtgagat gcggctcgac
     2101 gcgctgctcg accgctacgc gattgccgac cggctcatcc ggctggacct gatggggcag
     2161 gacgcgcgat cgagcgtccc gcacgtggtg ttccgcggta tcgagctcca cgacggccag
     2221 tggaacgaca tccccgtggc ggacgtcgat gccacgcagc aagatgccgc cctgaccggc
     2281 agcgatccga gctacgacgt gacgccggtc gcggaccgtc cgcagaacct cgcgttgacg
     2341 ctgacatccg atgcgttcaa cgcggcggcc ccggccgctc agcgcgacgc gctcgacgcg
     2401 ctggtcgcga cgcagaaccc ccagctccac acctcgagtg gcggatcgag cacggtgcag
     2461 tgcgttgcgt gccacgtggc gacgtatctc gccgtgcatc gcgcgcaggt cgcggccatc
     2521 gccctcgaca gcctgcccag ccggttccag accacgcatg acgcccgcat cacggacggc
     2581 atctcgacga ccactccggg ctcgctgcac aatttcggct ggctgggccc caacgtcgcg
     2641 atctcgcagc gcgcggcgaa cgagaccgcg atggtgctcg atgagatcca gcagcgcttt
     2701 cccgtcccgt gacgcccgcc cggccgtccg cggctccacc ggttgttgca gagctgtgga
     2761 ggcgtcggtg gttcgcccgg gcgccgccgc ggccgccgcg ctcgacccgt gtcacttcac
     2821 gtcgttcatc ggctacgagt ggaccaacaa ctgcaccggc gacggcggca gcgccgcgac
     2881 ctgtcacaag aacgtgatct tcggcgacac caacgtcccg gtcgcgccgt tcgactcgct
     2941 gtccaacccg acccaggagt cgctgtggag cgcgctcgat cacggctgca atggcggccg
     3001 cggatctccg tgcggatgta ccagacctgg gaccagacca ccgacttctg cgccgacccg
     3061 agcttcccgg cgcagatcgt cccaccagag ctcgcggttg gggtcggacg gttcgcgggt
     3121 cgccagcacg gcgcgcgccc ggccgccggc gtccacggtc accacctcga cggcgtggcg
     3181 gagttcgaca tcgaggctgg gatagcagca gccccgcgcg caggcgagcg ggcggtcccg
     3241 ggcgaccggt caccggaacg cggcgagcag ctcctcgggg atgcgctgcg gccggcccga
     3301 ggccaggccg atcatcgccc aggtggtcgc ggcgcgggcg acgacggtgc cgtcgcggac
     3361 gagctcggtc ttgcggatgc aggacgccgc gcgccagctg tcgacccagg tctcggcgcg
     3421 gagctcctgg ccgagcgtga cctgggcgat gtagtcgatc tcgtggcggc ggaccatcca
     3481 caccgcgccg agcgcgcggt aggcctgcat gtcccagccg cgggcgcgcg agtgggccat
     3541 ggcaacctct gccacccagc gcaggtacac caggttcgag acgtggccga gctcgtcgat
     3601 gtcctcgggg gtggggtgga tcgggatcgc aaaccgctcg gccatcgcgg cgtgtacatt
     3661 agcccggatg agcgacgaga ccacggtccg ggcgctgcgg gggcggctcg agcacgggca
     3721 ggcgaccgcg gccgaggcgg tggcgacgct cgcggtgtgg gcggtggagg cgccgacccg
     3781 ggcgacccgg ctcgacgcgt gccagctgct cggtgacctc gccggccggg cgctcggggc
     3841 cgagtgggag ctcgccgag
//
LOCUS       DASVOR010001981         2843 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341847,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001981 DASVOR010000000
VERSION     DASVOR010001981.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2843
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341847"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VF516_36765"
     CDS             complement(<1..354)
                     /locus_tag="VF516_36765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113358.1"
                     /translation="MSDPQATGPAGPDALRALIDRAFDYRGYVTLSRRDGGKLVGFVY
                     DRGPEHVELFDEAARCRIRLAVDQITDVEFTGEDSAANAQQIWERRKGALEPPDTPAW
                     GEWSARGDRRGARAAW"
     gene            complement(363..1379)
                     /gene="hpnH"
                     /locus_tag="VF516_36770"
     CDS             complement(363..1379)
                     /gene="hpnH"
                     /locus_tag="VF516_36770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HEX8113359.1"
                     /translation="MGIPLSQMFTVAHYVLKQRIAGRTRYPLVLMLEPLFRCNLACTG
                     CGKIQYPTALLKKHLSVDDCLAASDECGAPVVSVAGGEPLLHPQIAEIVRGLVARGRF
                     IYLCTNALLLEDKLGEFEPDAHLSFSIHMDGPRAEHDLSVCRAGVYDKAVSAIRAAVR
                     RGFRVTTNTTLFDGANPDRMRRFFDDMMALGIEGMMISPGYSYRKAPDQDHFLRREQS
                     HRLFSRMLESPARRWRFNQSPLFLQFLMGKREFDCTPWGNPTYNIFGWQRPCYLLQDG
                     YARTFRELMDQTEWSRYGHASGNPRCRDCMVHCGFEPSAINASFGSLRGMGETALALV
                     KERR"
     gene            complement(1629..>2843)
                     /locus_tag="VF516_36775"
     CDS             complement(1629..>2843)
                     /locus_tag="VF516_36775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113360.1"
                     /translation="PMTARTAVRRIAIAVASAAAIAVASAARAECAGDQETKTVPLPV
                     WATSPNEGNTWGAMPVFLRVCAADQSTQWLIAPSVTWNSVIHTTASLRFYDYPDPDTV
                     LSVIASASTRINYRLVARWQRLPADDGTWTDEAALDVERSAFARFFGLGPDAPARAET
                     SYTSARVLVSERRGRNLIDHVNAGILVGFEHDAIEDRGVPGLPLAPERFPGVPGMRGS
                     TVLWQGIDLRYDDREGGDYAEQGVRLEASLAVIEGLVRSPTFVRVGAQARGILPELAW
                     LSGAARAMWTAVSNPGAPFYLQSWLGGSFALRGFPDGRFIDRQAWTIEGEQRIRLVQT
                     HLMGVAVDWRADPFIAVGQVFHGLGSALSRPRLTGGVGLRAVVHPSLVGRVDLASGGE
                     GLSVYAEIGYPY"
BASE COUNT          434 a         1052 c          957 g          400 t
ORIGIN      
        1 ccacgcggcg cgcgcacctc gccggtcgcc gcgcgcgctc cactcgcccc acgccggggt
       61 gtccggcggc tccagcgcgc ccttgcggcg ctcccagatc tgctgggcat tcgccgcgct
      121 gtcctcgccg gtgaactcga cgtccgtgat ctgatcgacc gcgaggcgga tccggcaccg
      181 cgcggcctcg tcgaacagct cgacgtgctc cgggccgcga tcgtagacga acccgaccag
      241 cttgccgccg tcccgccgcg acagcgtgac gtagccgcgg taatcgaacg cgcggtcgat
      301 cagcgcgcgc agcgcgtccg gtccggccgg gccggtggcc tgcggatcgc tcatcggctc
      361 tctcatcgcc gctccttgac cagcgccagg gccgtctcgc ccatcccgcg cagcgagccg
      421 aaactggcat tgatcgcgct gggctcgaat ccgcagtgca ccatgcagtc gcggcagcgc
      481 gggttgccgc tcgcgtggcc gtacctcgac cactcggtct gatccatcag ctcgcggaac
      541 gtccgcgcgt agccgtcctg cagcaggtag cacggccgct gccagccgaa gatgttgtac
      601 gtcggattgc cccacggcgt gcagtcgaac tcgcgcttgc ccatgaggaa ctgcaggaac
      661 agcggagact gattgaaccg ccagcgccgg gccggcgact ccagcatccg ggagaacagc
      721 cggtgcgact gctcgcggcg caggaagtgg tcctgatcgg gcgccttgcg gtacgagtag
      781 cccggcgaga tcatcatgcc ctcgatcccg agcgccatca tgtcgtcgaa gaaccgccgc
      841 atccggtccg ggttcgcgcc gtcgaacagc gtggtgttgg tggtgacccg gaacccgcgg
      901 cggaccgccg cccggatcgc cgagaccgcc ttgtcgtaga cccccgcccg gcacaccgag
      961 aggtcgtgct cggcgcgcgg gccgtccatg tggatgctga agctgaggtg cgcgtcgggc
     1021 tcgaactcgc cgagcttgtc ctccagcagc agcgcgttgg tgcacaggta gatgaagcgg
     1081 ccgcgcgcga ccaggccgcg cacgatctcg gcgatctgcg gatgcagcag gggctcgccg
     1141 ccggcgacgc tgaccaccgg cgccccgcac tcgtcggacg ccgccaggca gtcgtcgacc
     1201 gacaggtgct ttttcaacag cgcagtcgga tactggatct tgccacagcc ggtgcaggcc
     1261 agattgcagc gaaacagcgg ctccagcatc agcacgagcg ggtagcgcgt gcggcccgcg
     1321 atgcgctgct tcaacacgta gtgcgccacg gtgaacatct gggacaacgg aatccccatg
     1381 aggcaaggcc ttccttcctg agctggcgcc ccgcgccaaa caacgatggt gattccctat
     1441 tacctaccct cgatgacgac attggcaatg tcaagattga cccctagccg ctttttcagc
     1501 attgcaagac ggtgagcgcc cgcgagcttc agaacctcgc cgatcccggc gcccgcgctc
     1561 gatcgatagg ggcgcgcgtc gcgtcgcgcg gctgagctcg catggccacg gatcgacgcg
     1621 agcatcctct agtacgggta gccgatctcg gcgtacaccg agaggccctc gccgccgctc
     1681 gcgaggtcga cgcggccgac caggctgggg tggacgaccg cgcgcagccc gacgccgccg
     1741 gtcagccgcg ggcgcgacag cgcgctgccg aggccgtgga acacctggcc caccgcgatg
     1801 aacggatccg cgcgccagtc gaccgccacg cccatgaggt gggtctggac cagccggatc
     1861 cgctgctcgc cctcgatcgt ccaggcctgg cgatcgatga accggccgtc gggaaagccg
     1921 cgcagcgcga acgagcctcc gagccagctc tggaggtaga acggcgcgcc cgggttgctg
     1981 accgcggtcc acatggcgcg cgccgcgccc gacagccacg ccagctcggg caggatgccg
     2041 cgcgcctgtg cgccgacgcg cacgaacgtc ggcgagcgga ccagcccctc gatcacggcg
     2101 agcgacgcct ccagccgcac gccctgctcg gcgtagtccc cgccctcgcg atcgtcgtag
     2161 cgcagatcga tgccctgcca cagcacggtc gagccgcgca tccccgggac gccggggaac
     2221 cgctcgggcg cgagcggcaa ccccggaacg ccgcgatcct cgatcgcatc gtgctcgaaa
     2281 ccgacgagga tcccggcgtt gacgtggtcg atcaggttgc gcccccggcg ctcgctgacc
     2341 agcacccgcg cgctggtgta gctggtctct gcgcgggccg gcgcgtcggg gcccagcccg
     2401 aagaaccgcg cgaacgcgct gcgctcgacg tcgagcgcgg cctcgtcggt ccacgtcccg
     2461 tcgtcggccg gcagccgctg ccacctcgcc accagccggt agttgatccg cgtgctcgcc
     2521 gaggcgatca ccgacagcac ggtgtccgga tccgggtagt cgtagaaccg cagcgaggcg
     2581 gtggtgtgga tcaccgagtt ccaggtcacg ctcggcgcga tcagccactg cgtggactgg
     2641 tcggcggcgc acacccggag gaacaccggc atcgcgcccc acgtgttgcc ctcgttgggc
     2701 gacgtcgccc acaccggcag cgggaccgtc ttggtctcct gatcgcccgc gcactcggcg
     2761 cgcgccgccg atgcgaccgc gatcgccgcc gcggatgcga ccgcgatcgc gatccgcctg
     2821 accgccgtgc gcgcggtcat cgg
//
LOCUS       DASVOR010001982         9067 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_371632,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001982 DASVOR010000000
VERSION     DASVOR010001982.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9067
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371632"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VF516_36780"
     CDS             complement(<1..638)
                     /locus_tag="VF516_36780"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8113361.1"
                     /translation="MSSSDSLADAELPDINDRLVEPDTPYEIWDGEVVYVSPAHPPHG
                     QRQRQLCALIDAHTGPEFQSAADLLTRTSRIDDIAPDVSVYPAAPNPATGGRQLEHLA
                     FEIVSKQSLRRAGRKAAKLAGRGVRRVFAIDIKRSRALEWSAEAGAWRELDPAGHIED
                     PALAVPLPIASLIHDARPDDAVARALLAKRNPVLMQERAEGHAESRAEGRAEA"
     gene            920..1933
                     /locus_tag="VF516_36785"
     CDS             920..1933
                     /locus_tag="VF516_36785"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8113362.1"
                     /translation="MRIQVVAIALLIGCHGGPEHSGELADATATDPLKQIFDEVDGAR
                     ITQVMRELSGVAPVTVNGASITLGERFDDAGRQRFRDYWIQTMRNLGLEINPLHYQAA
                     GHPRPGDDVEAILRGPSADSLVVIVHYDSIGPAGSETSNPGADDDMSGMAILLETARL
                     FVQHRAELALTVRFVASDEEELGGLAGARNYAAYIQALSRSEGFALVAAVDDEQSGWN
                     CSTDGLCGDDLFPAFDVFSCGSSAATSFDFAALGDQLAGIAARYSTLHVKRGCIGASS
                     DHFAMWEIGVPAVVYSEHNAFANPRFDRNGGDVFARIDTDYLTSIARPAIAFQAALAG
                     VHK"
     gene            2103..3794
                     /locus_tag="VF516_36790"
     CDS             2103..3794
                     /locus_tag="VF516_36790"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8113363.1"
                     /translation="MPLVFVGVDVFIIHAAPDTETALLLREALAEIGVSAFVDKSDAP
                     AGEPWDVAMLHELRQSLLQVVLLGESIRWYQHEEIDIAIREMRAHDADDRVVPVLLKK
                     RALLPYGLNRVQALDWDGPNRVAARLGKKVAALRGIPMIAGRLATSQRQVKIAVLGFH
                     AELPEALETLRRRIGQRTAILDGEVQRVDLMRDAVPEVDLRVVLLGSQHGGKLALIHS
                     LVAADRRDAAARDTIVGAIDVPLRQVPGAHKAEAIDMQSLDVAITSTSIESVIDALDD
                     NVASWHAARFPSHPGMSVTREPWEKRYLADKAPLWRKGDPSALRALGGARVDRIRWYV
                     HLKAGDSWSELQPWLDWALSRESVPRCVVVGSAGTGKTVLLQHLAWVLATDDEPGPPD
                     EPVHRVDRAGVRGKSPTRPVPILHSAATLAAYLRGDRPIREVLVELPCSSETFGRDVD
                     AAALRGGIDVGRYVFLIDSLDEVPRREDREALVAALGTLTGPSPRVILTTRPSAYTAV
                     ELPEGFERVDIAPLDDDAIRRLTQAWIACWTPRCRSSGCAERGRRREERLSGANR"
     gene            3742..4701
                     /locus_tag="VF516_36795"
     CDS             3742..4701
                     /locus_tag="VF516_36795"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8113364.1"
                     /translation="MLNAVDGVKSDFPAQTDERSPVENPLLLSCVLQVYATYRRLPDD
                     AAKLYHDMVRVLCEAKIRAADDRARDTLVEEYQSALRELFLACQEFGSTRLGVTAAHE
                     LLVDRNFVSRSAAENWLRGLANHTGLVRFEGTGDHTVIPPWHRSYQEYLAAESIALTY
                     ANRATECIAWLRSAPATRGPRLSDPAWRGTLRFLIGAAVHQKRDWGVDLLDALGPVSA
                     FPRGHTPEGIWDMAGNVFEWCASRRVYGMIHDLPVDMEGGPEARILRGGCWRSPIAPL
                     VVRGERPGQTDDDIGFRFICRPLQATPQVRGSRRRHGRDPDTG"
     gene            complement(4738..5784)
                     /locus_tag="VF516_36800"
     CDS             complement(4738..5784)
                     /locus_tag="VF516_36800"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEX8113365.1"
                     /translation="MKTRAYAARSATTALEPFEISRREPGALDVAIDILFCGVCHSDI
                     HQVRGEWNNSEFPMVPGHEIIGRVARVGDQVTSVAAGDLVGVGCIVDSCRTCGPCRRD
                     HEQFCTAGPAWTYNSTEMDRTTPTHGGYSQHVVVTERFVAKLPAGLDPAAAAPLLCAG
                     ITTYSPLRQWDCKPGDRVAVVGLGGLGHMAVKLAAALGAEVTVLSTSPAKAADARRLG
                     ASAFVDLGDARARDALRDAIDLVIDTVSAQHDYNALLQLLRPQGALALVGMPPRQGAA
                     FDPFLLIDANRRLAGSSIGGMKETHDMLAFCAQHGIVSDVELIPIQRINQAFERVIRS
                     DVRYRFVIDIASLG"
     gene            5887..6273
                     /locus_tag="VF516_36805"
     CDS             5887..6273
                     /locus_tag="VF516_36805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113366.1"
                     /translation="MTRILLIGLEPTLVDFSSMPDLDAAKVAQGLRAQEQSLRDLGFD
                     AAWCLTDLGATAEAVVRAALAAKPSDVVLIGAGIRVLPAHFLLFERLINVVHEGAPRA
                     KICFNTRPDDTQEAVLRWIAPLGGAP"
     gene            complement(6282..6731)
                     /locus_tag="VF516_36810"
     CDS             complement(6282..6731)
                     /locus_tag="VF516_36810"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8113367.1"
                     /translation="MARRLLQHLRPLSLQDQVCAWSDKDIAAGERWQDVVEFSLGNAR
                     AAVLLVSPAFLASEFIRNSELPVLLQAAKERGVTIIPVILRPCLFKETSFRYPDPKTG
                     PQTLSLASLQAPGSPEQALNELSEGAQDTVLLGVARRLLALVRGPTG"
     gene            complement(6897..7997)
                     /locus_tag="VF516_36815"
     CDS             complement(6897..7997)
                     /locus_tag="VF516_36815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX8113368.1"
                     /translation="MTAPSIAALFDLLLDRGGSDLHLAAGYPPMLRVRGDLVPEGDRV
                     LSAADVDALLAPLLSRDQAAQFAATGDLDFAHAHGSRARFRANYLRKASGPGAVFRAI
                     PSEIQSLDALGAPAGIRKLAELRAGLVLVTGPTGSGKSTTLAAMIDHINRSRAGHILT
                     IEDPVEFVHPPRRCLVTHKEVGDHVPSFLEAIRSAGRENADVILVGELRGAETMKAAL
                     QLASFGILIFATVHTNSAGATIDRFVNAFPAEQQPQIRGLLADSLAGVVAQQLLRRAD
                     VKGRVAAHEILLGNYALASLIREGKTSQIASFIQSSSGEGMQSMDSALERLVAAGTIS
                     AKDALDKALDKEAFVKLPAIARALGPEAALTA"
     gene            complement(7994..>9067)
                     /locus_tag="VF516_36820"
     CDS             complement(7994..>9067)
                     /locus_tag="VF516_36820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEX8113369.1"
                     /translation="NATDLHVAANRPVLVRTLGELVALDPAPRPAAEIEALVLPLLGP
                     TRRQQLAERGYVDLAVDVPGGGRLRANISRQQGGLKGAFRLAVAVPLSLVELGLPAAV
                     AKVTEHHQGLIVIAGPSGHGKTTTLAALVDLLNRARPIHILTIEDPVEIVHPRHAAVV
                     SQREVGRHTLSFAAALKASLREDPDVIVIGELRDRETVEIALTAAETGHLVLATMSTP
                     SAAKTIDRLVDMFPPDDQSQVRASLGGALRAIVAQRLLPDAQGTGMVAAVELLVGVLA
                     LANLIRDNKLFQLPNLMQRGRAFGMLRLDDSLLDLVRAGKLTEDVALRHADNRKELTA
                     ALHPPPPPAEPVKRSLFGRKERE"
BASE COUNT         1358 a         2999 c         3311 g         1399 t
ORIGIN      
        1 gcctccgcgc ggccctcggc cctgctttcg gcgtggccct ccgcgcgctc ctgcatcagc
       61 accgggttgc gcttggcgag cagggcgcgg gcgacggcat cgtcgggcct ggcgtcgtgg
      121 atcagcgacg cgatcggcag cgggaccgcg agcgccggat cctcgatgtg gccggccggg
      181 tcgagctcgc gccacgcgcc ggcctcggca gaccactcga gcgcgcgcga tcgtttgatg
      241 tcgatcgcga acacgcggcg cacgccgcgg ccggccagct tggcggcctt ccggccggcg
      301 cggcggagcg actgcttgct gacgatctcg aacgcgagat gctcgagctg acggccgccg
      361 gtcgcagggt tcggcgcggc ggggtacacg ctgacgtcgg gcgcgatgtc gtcgatccgc
      421 gaggtccggg tcaacaggtc cgccgcggac tggaactcgg gtccggtgtg ggcgtcgatc
      481 agggcgcaca gctgtcgctg ccgctgcccg tgtggtggat gggcggggga cacgtagact
      541 acctccccgt cccagatctc gtacggagtg tcgggctcga cgaggcggtc gttgatgtcg
      601 gggagctcgg cgtcggcgag ggagtcgctg gacgacatgc cccagcatag cgctgcggga
      661 ccccaggatg gatcggagct cgaacgatgc atggcaacac catgcggcgg ccgagagcaa
      721 acgccgcgcc gcacgcaacc gtgcgagatc acgcgccgcg ctgtccggcg aatggctgga
      781 ttccggccgg tcgtccgagc ttcggacaaa cgccggacag gcacgctgcg ccgacccgcg
      841 cgagatccgg gcaccggcgg tgccctcgcc gcgggcccgt gctgctgacc gcggggcgca
      901 agccgcgtac tctcccggca tgcgaatcca ggtggtggcg atcgcgctcc tgatcggctg
      961 tcacggtggc ccggagcact ccggggagct cgccgatgcg accgccacgg atcctctcaa
     1021 gcagatcttc gacgaggtcg atggcgcccg gatcacgcag gtgatgcgcg agctgtcggg
     1081 cgtggcgccg gtcaccgtca acggcgcgtc gatcacgctg ggcgagcgat tcgacgacgc
     1141 cggccgccag aggttccgtg actactggat ccagacgatg cggaacctcg gcctcgagat
     1201 caacccgctc cactaccagg cggccggcca cccgcgccct ggtgacgacg tcgaggccat
     1261 cctgcgcgga ccgtcggccg attcgctcgt cgtgatcgtc cactacgaca gcatcggccc
     1321 ggccggcagc gagacctcca acccgggggc agacgacgac atgtcgggga tggcgatcct
     1381 gctggagacc gcgcggctgt tcgtccagca ccgcgccgag ctcgcgctga ccgtacggtt
     1441 cgtcgccagt gacgaggagg agctcggcgg tctcgccggc gcgcgtaact acgccgcgta
     1501 catccaggcg ctgtcgcgga gcgagggctt cgccctggtc gccgcggtcg acgacgagca
     1561 gagcggctgg aactgcagca cggacggcct gtgcggcgac gacctgttcc cggcgttcga
     1621 cgtgttcagc tgcggcagca gcgccgcgac cagcttcgac ttcgcggcgc tcggcgatca
     1681 gctcgccggc atcgccgcgc gctactcgac gctccacgtg aagcgcggct gcatcggcgc
     1741 cagcagcgac cacttcgcga tgtgggagat cggcgtgccc gcggtggtct actccgagca
     1801 caacgcgttc gcgaatccgc gcttcgaccg caacggcggc gatgtgttcg ccaggatcga
     1861 taccgactac ctgacgtcga tcgcgcggcc ggcgatcgcg ttccaggccg cgctcgccgg
     1921 ggtccacaag tgatcccgcc tgacgcaatg acgccgcggg atgccgggcg cgctgactcg
     1981 cgcgtgcagc ggccagcgct gggcaccatc ctgaggaatc acgccgacgt acgtgagccc
     2041 gcgaccgccc gccgaccacc gtagctggct gcccccagac ctccagcttg tgcggtgata
     2101 tcgtgccgct ggtcttcgtg ggcgtcgacg tcttcatcat acatgcagca ccggatactg
     2161 agacggcgct cctgctccgc gaggcgctcg ccgagatcgg cgtgagcgcg ttcgtcgata
     2221 aatccgatgc tccggcgggc gaaccatggg acgtggcgat gctccacgag cttcggcaat
     2281 cacttctgca agtggtgctg ctcggcgaga gtatccgctg gtaccaacac gaggagatcg
     2341 acatcgcgat ccgcgagatg cgagcccacg acgctgacga tcgcgtcgtt ccggtgctct
     2401 tgaagaagcg agcactcctg ccctacggac tcaaccgcgt acaagcgctg gactgggatg
     2461 gcccgaacag ggttgccgcc aggctcggca agaaagtcgc ggctctgcgg gggataccga
     2521 tgatcgctgg caggcttgcg acgtcgcagc gccaggtgaa gatcgctgtg ctcggcttcc
     2581 acgccgagct gccggaagcg ctcgagacac tgcgtcggcg catcggtcag cgcacggcga
     2641 tcctcgacgg cgaggtgcag cgggtagacc tgatgcgtga tgcggtgccc gaggtcgacc
     2701 tgcgtgtggt gctgctcggc agtcagcacg gtggcaagct cgccctcatc catagcctgg
     2761 tggccgcgga ccggcgggac gccgcagctc gcgacacgat cgtcggggcg atcgatgtgc
     2821 cgctgcgaca ggtccctggg gctcacaagg ccgaagcaat cgacatgcaa tcgctagacg
     2881 tggcgatcac gtcgacctcg atagagtcgg tcatcgacgc cctcgatgat aacgtggcga
     2941 gctggcacgc ggcgcgcttt ccgtcgcacc cgggaatgtc ggtcacgcgc gagccctggg
     3001 agaagcgcta tctcgccgac aaggcgccac tctggcgcaa aggcgatccg agcgcgcttc
     3061 gcgcactcgg tggtgcacgt gtggatcgca ttcgctggta tgttcacctc aaggcaggtg
     3121 actcgtggag tgagctgcaa ccttggctcg attgggcgct gagcagggaa tcggtgccac
     3181 gatgtgtcgt ggttggctcc gctggcaccg ggaagaccgt gctgctgcag cacctcgcgt
     3241 gggtgctcgc gaccgatgac gagccaggtc ccccggacga acccgtgcat cgagtcgacc
     3301 gcgccggtgt tcgcggcaag agcccgacgc ggccggtgcc catcttgcac agcgccgcga
     3361 cgctggcagc ctacctgcgc ggcgatcggc cgatccgcga ggtgctggtg gagttgccgt
     3421 gctcgagtga aacgttcggt cgcgatgttg acgctgcggc cctgcgcgga gggatcgacg
     3481 tggggcgcta cgtgttcttg atcgactcgc tcgacgaggt gccccggcgg gaggatcgcg
     3541 aagcactggt ggctgcgctc ggaacgctga ccggtccctc gccgcgcgtc atcctcacta
     3601 cccgccccag cgcgtacacc gcagtcgaac tgcccgaagg cttcgagcgc gtcgacattg
     3661 caccgctcga cgacgatgcg atccggcgtc tgacccaggc gtggatcgca tgctggaccc
     3721 cccgatgcag atcctcagga tgtgctgaac gcggtcgacg gcgtgaagag cgactttccg
     3781 gcgcaaaccg atgagcgctc gccagtcgag aatccgctgt tactgagctg tgtcctgcag
     3841 gtgtatgcga cctatcggcg cctgcccgac gacgctgcga agctgtacca cgatatggtg
     3901 cgtgttctgt gcgaggcgaa gattcgcgca gccgacgatc gtgctcgcga cactctcgtc
     3961 gaggagtacc aaagtgcgct gcgcgagctg ttccttgcct gtcaggagtt cggtagtacg
     4021 cggctgggcg tcactgctgc acatgaactg ctcgtcgacc gtaacttcgt gtcacgcagt
     4081 gccgctgaga actggctccg gggcctggcc aatcacacgg gcctggtgcg ttttgagggt
     4141 actggtgatc atacggtgat ccctccctgg catcgcagct atcaggagta cctggccgca
     4201 gagtcgatcg cgctgacgta cgcgaaccgt gcgaccgagt gcatcgcctg gctacgcagc
     4261 gcgccggcga ctcgcggccc gcgactcagc gatccggcgt ggcgtggcac gctgcggttc
     4321 ttgatcggcg cggcggttca tcagaagcgt gactggggcg tcgatctgct cgacgcgctg
     4381 gggccggtca gtgcattccc tcgcgggcac acacctgaag gaatctggga catggcagga
     4441 aacgtcttcg agtggtgcgc gtctcggcgg gtctatggca tgatccacga tttgcccgtc
     4501 gatatggagg gcgggcccga agcgaggatc ttgcgcggcg gctgctggcg gtcccccatt
     4561 gccccactgg tcgttcgtgg tgagaggccc ggccagaccg atgacgacat tggctttcgc
     4621 tttatctgtc ggccgttgca ggcaacaccg caagtccgcg gcagcagacg tcgacacggt
     4681 cgagacccag acaccggatg aatgcggcga tctcgacatg ccgccgatcg cgggcgttta
     4741 cccgagcgac gcgatgtcga tgacgaagcg atagcggacg tcgctccgga tcacgcgctc
     4801 gaacgcctgg ttgatccgct ggatcgggat cagctcgaca tcggacacga taccgtgctg
     4861 cgcgcagaac gcgagcatgt cgtgggtctc cttcatgccg ccgatcgagg agccggcgag
     4921 gcggcggttg gcgtcgatca gcaggaacgg atcgaaggcg gcgccctggc gcggcggcat
     4981 gccgacgagc gccagcgcgc cctgcggccg gagcagctgg agcagcgcgt tgtaatcgtg
     5041 ctgcgccgac accgtatcga tgacgaggtc gatcgcgtcg cgcagcgcgt cgcgcgcgcg
     5101 cgcatcgccg aggtcgacga aggcgctcgc gccgagccgc cgggcgtcgg ctgccttggc
     5161 gggtgacgtg ctgagcacgg tcacctcggc gcccagcgcg gcggcgagct tcaccgccat
     5221 gtggccgagc ccgccgagcc cgacgacggc gacccgatcg ccaggcttgc aatcccactg
     5281 cctgagcggc gagtacgtcg tgatccccgc gcacagcagc ggcgccgcgg ccgccggatc
     5341 gagcccggcg ggcagcttcg cgacgaaccg ctcggtcacc acgacgtgct gcgagtagcc
     5401 gccgtgggtc ggcgtggtgc gatccatctc ggtcgagttg taggtccacg ccggtccggc
     5461 cgtgcagaac tgctcgtgat cgcgccggca cgggccgcac gtgcggcacg agtcgacgat
     5521 gcagccgacg ccgaccagat cgccggccgc cacgctcgtg acctgatcgc cgacgcgcgc
     5581 gacccggccg atgatctcgt ggccgggcac catcgggaat tctgaattat tccactcgcc
     5641 gcgcacctgg tggatgtccg agtggcacac gccgcagaac aggatgtcga tcgcgacgtc
     5701 gagcgcgccg ggctcgcggc gagagatctc gaacggctcg agcgcggtgg tcgccgaccg
     5761 tgcggcgtag gccctggtct tcatcgccgc cagtctacgc gctccgcgcg gcgccgatcc
     5821 ggagtgccgg gatctgatag gagagcccgg acaccagatg cgccgcgctg gctatgctcg
     5881 gtggcgatga cgcggatcct gctgatcggc ctcgagccca cgctggtgga cttctcgtcc
     5941 atgcccgatc tcgacgcggc gaaggtcgcg cagggcctcc gggcgcagga gcaaagcctg
     6001 cgcgacctcg gcttcgacgc cgcgtggtgt cttaccgatc tcggtgcgac ggccgaggcg
     6061 gtcgtgcgcg ctgcgctcgc cgcgaagccg tccgacgtcg tcttgatcgg tgcgggcatc
     6121 cgcgtgctgc cggcgcactt cttgctgttc gagcgcctca tcaacgtcgt gcatgagggc
     6181 gcgccgcggg cgaagatctg cttcaacacg cgccccgatg acacccagga ggcggtgctg
     6241 cgctggatcg ccccgctcgg cggcgctccc tgaccgaccg ctcaacccgt ggggccgcgt
     6301 acgagcgcca gcagccgtct cgcgacgccc agcaggaccg tatcctgggc gccctcgctg
     6361 agctcgttca gcgcctgttc cggcgagccg ggcgcctgaa ggctggcgag agacagcgtc
     6421 tgcggaccgg tcttgggatc gggatacctg aacgacgtct ctttgaatag acatggacga
     6481 aggatcaccg gaatgatggt gactccacgt tccttcgccg cctggagcag cacgggcagc
     6541 tcgctgttgc ggatgaactc cgaggcgagg aacgccgggc tcaccaggag gaccgcagcg
     6601 cgtgcattac cgaggctgaa ctcaacgacg tcctgccatc gttcgcccgc ggcgatgtct
     6661 ttgtcggacc aggcacagac ctggtcctgc agtgagagcg gtcgcagatg ctggagcagg
     6721 cgtcgagcca gcgcttctcc cgatcggggc tctcgttgtc ctcatgcgca tagctcacga
     6781 acaccggcaa tcggctcggc accatgaccg cagactagac cgtcctcggc gccgagtcga
     6841 aggcttgtcg atgatcgctt cccgtggact gccgcgaaca gaaagtcgcg gcgcggtcag
     6901 gcggtcagcg cggcctcggg gccgagcgcg cgggcgatcg ccggcagctt gacgaacgcc
     6961 tccttgtcga gcgccttgtc gagcgcgtcc ttggccgaga tcgtgccggc ggcgaccagg
     7021 cgctcgagcg cgctgtccat gctctgcatg ccctcgccgg agctcgactg gatgaagctg
     7081 gcgatctggg aggtcttgcc ctcgcggatc agcgacgcga gggcgtagtt gccgagcagg
     7141 atctcgtggg cggcgacccg gcccttgacg tcggcgcggc gcagcagctg ctgggcgacc
     7201 acgccggcga gcgagtcggc gagcaggccg cggatctggg gctgctgctc ggcggggaac
     7261 gcgttgacga agcgatcgat cgtcgcgccg gccgagttgg tgtggacggt ggcgaagatc
     7321 aggatgccga agctggcgag ctggagcgcg gccttcatgg tctcggcgcc gcgcagctcg
     7381 ccgaccagga tgacgtcggc gttctcgcgg ccggcgctgc ggatcgcctc gaggaagctc
     7441 ggcacgtggt cgccgacctc cttgtgggtg accaggcagc gccgcggcgg gtggacgaac
     7501 tcgaccgggt cctcgatcgt gaggatgtgg ccggcgcggc tgcggttgat gtggtcgatc
     7561 atcgcggcga gcgtggtcga cttgccgctg ccggtcgggc cggtgacgag gaccaggccg
     7621 gcgcggagct cggcgagctt gcggatgccg gcgggggcac cgagcgcgtc gagcgactgg
     7681 atctcgctcg ggatcgcgcg gaacaccgcg cccgggccgg aggccttgcg caggtagttg
     7741 gcgcggaacc gggcgcgcga gccgtgggcg tgggcgaagt cgagatcgcc ggtcgccgcg
     7801 aactgcgcgg cctggtcgcg cgacaggagc ggcgcgagca gcgcgtcgac gtcggccgcc
     7861 gacagcacgc gatcgccctc ggggacgaga tcgccgcgca cgcgcagcat cggcgggtag
     7921 ccggcggcga ggtggaggtc ggatccgccg cggtcgagca ggaggtcgaa cagggcggcg
     7981 atcgaggggg cggtcattcg cgctccttgc ggccgaacag gctgcgcttg acgggctcgg
     8041 cgggcggcgg gggcgggtgg agcgcggcgg tgagctcctt ccggttgtcg gcgtggcgca
     8101 gcgcgacgtc ctcggtgagc ttgccggcgc ggaccaggtc gagcagcgaa tcgtcgagcc
     8161 gcagcatgcc gaacgcgcgg ccgcgctgca tcaggttggg gagctggaac agcttgttgt
     8221 cgcggatcag gttggcgagc gcgagcacgc cgacgagcag ctcgacggcg gcgaccatgc
     8281 cggtgccctg ggcgtcgggc aacaggcgct gggcgacgat cgcccgcagc gcgccgccga
     8341 gcgaggcgcg gacctgcgac tggtcgtcgg gcgggaacat gtcgaccagc cggtcgatcg
     8401 tcttggccgc cgacggcgtg ctcatcgtcg cgagcacgag gtggccggtc tcggcggcgg
     8461 tcagcgcgat ctcgacggtc tcgcggtcgc gcagctcgcc gatcacgatg acgtcggggt
     8521 cctcgcgcag cgacgccttg agcgcggcgg cgaacgacag ggtgtggcgg ccgacctcgc
     8581 gctgcgacac cacggcggcg tggcgcgggt ggacgatctc gaccgggtcc tcgatggtca
     8641 ggatgtggat cggacgggcg cggttgagca ggtcgaccag cgccgcgagc gtcgtggtct
     8701 tgccgtggcc gctcgggccg gcgatcacga tcaggccctg gtggtgctcg gtgaccttgg
     8761 cgaccgcggc gggcaggccg agctcgacga gcgacagcgg cacggcgacc gcgagccgga
     8821 acgcgccctt gaggccgccc tgctggcgcg agatgttggc gcgcagccgg ccgccgcccg
     8881 gcacgtcgac cgcgagatcg acgtagccgc gctcggcgag ctgctggcgc cgggtcgggc
     8941 cgagcagcgg caggaccagg gcctcgatct cggcggcggg gcgcggcgcc gggtcgagcg
     9001 cgaccagctc gccgagcgtg cggaccagca cgggccggtt cgccgcgacg tggagatcgg
     9061 tggcgtt
//
LOCUS       DASVOR010001983         1559 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341869,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001983 DASVOR010000000
VERSION     DASVOR010001983.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341869"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..>1559)
                     /locus_tag="VF516_36825"
     CDS             complement(96..>1559)
                     /locus_tag="VF516_36825"
                     /inference="COORDINATES: protein motif:HMM:NF024924.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEX8113370.1"
                     /translation="QFGPELVEAFREFKRIWDPDDRMNPGKIVDGLHRTDDLKLAAYH
                     PPEITTMFHPAEDHGDFRHAAIRCVGIGKCRRIGGGTMCPSFMVTREEKHTTRGRAHL
                     LFEMVQGEVIQDGWASDEVKDALDLCLACKGCKGDCPVHVDMATYKSEVLAHYYRHHG
                     RPRHAYAFGWIHRWALLASYAPWLVNFATQTPGLRRIAAWLAGAAPHRPIPRFAARPF
                     SRSFVPRRTDRVPVVLWPDTFNNYFFPETLDAAAKVLEDAGCRVAVPRAHVCCGRALY
                     DYGMLGLARRLWHRTFRILEPAIARGVPIVGLEPSCVAAFRDELPSLFPGDDRARRLS
                     DSTHTLAGFLRARGYQPPPLPGRALLHGHCHHKAVLDFEDERALLADMQLDLESPDSG
                     CCGLAGSFGFEPEHYEISMAIGERALLPAVRAERPDGLVIADGFSCREQIRHGTGRTA
                     LHVAEVVALALRALERTRAPERRLRVEPATALTAARS"
BASE COUNT          215 a          551 c          587 g          206 t
ORIGIN      
        1 ctctccgacc tgccgccgca tcgacgatgc gaccgcgacg gcggctgggc ccgtgtctca
       61 gccgaaagag ctgatgttcc acatggcagc atcgatcagc ttcgtgcggc ggtgagcgcc
      121 gtcgccggct cgacccggag ccggcgctcc ggcgcgcggg tgcgctcgag cgcgcgcagc
      181 gcgagggcga cgacctccgc cacgtgcagc gcggtccgcc cggtgccgtg ccggatctgc
      241 tcgcggcacg agaagccgtc ggcgatcacc aggccgtccg ggcgctcggc gcgcaccgcc
      301 ggcagcagcg cgcgctcgcc gatcgccatc gagatctcgt agtgctcggg ctcgaacccg
      361 aacgagccgg cgagcccgca gcatccggag tccgggctct cgagatcgag ctgcatgtcg
      421 gcgagcagcg cgcgctcgtc ctcgaagtcg agcaccgcct tgtggtggca gtggccgtgc
      481 agcagcgcgc ggcccggcag cggcggcggc tggtaaccgc gggcgcgcag gaagccggcc
      541 agcgtgtggg tggagtccga cagccggcgg gcgcggtcgt cgcccgggaa caggctcggc
      601 agctcgtcgc ggaacgccgc gacgcagctc ggctcgaggc cgacgatcgg gacgccgcgc
      661 gcgatcgcgg gctcgaggat ccggaacgtc cggtgccaca gccggcgggc caggccgagc
      721 atgccgtagt cgtacagcgc gcggccgcag cacacgtggg cgcgcggcac ggccacgcgg
      781 cagccggcgt cctcgagcac cttggccgcg gcgtccagcg tctcggggaa gaagtagttg
      841 ttgaacgtgt cgggccacag caccacgggc acgcggtcgg tgcgccgggg cacgaagctg
      901 cggctgaacg gccgcgcggc gaaccgcggg atcgggcgat gcggcgcggc gcccgccagc
      961 cacgccgcga tccggcgcag cccgggggtc tgggtcgcga agttgaccag ccacggcgcg
     1021 tagctggcga gcagcgccca gcggtggatc cagccgaacg cgtaggcgtg gcgcgggcgc
     1081 ccgtgatggc ggtagtagtg cgcgaggacc tccgacttgt acgtcgccat gtcgacgtgc
     1141 accggacagt cgcccttgca gcccttgcac gccaggcaca gatcgagcgc gtccttgacc
     1201 tcgtcgctgg cccagccgtc ctggatgacc tcgccctgca ccatctcgaa cagcaggtgc
     1261 gcgcggccgc gcgtggtgtg cttctcctcg cgcgtgacca tgaagctcgg gcacatcgtc
     1321 ccgccgccga tgcgacggca cttgccgatg ccgacgcacc ggatcgcggc gtgccggaag
     1381 tcgccgtggt cctcggcggg gtggaacatc gtcgtgatct cgggcgggtg gtacgcggcg
     1441 agcttcaggt cgtcggtgcg gtggagcccg tcgacgatct tgccggggtt catccgatcg
     1501 tcggggtccc agatccgctt gaactcgcgg aacgcctcga ccagctccgg cccgaactg
//
LOCUS       DASVOR010001984         2493 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_429368,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001984 DASVOR010000000
VERSION     DASVOR010001984.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429368"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="VF516_36830"
     CDS             complement(<1..787)
                     /locus_tag="VF516_36830"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113371.1"
                     /translation="MGSDREQELARTATAAASAPAEPAEPFGANLGRYRLERELGAGA
                     MGVVHAAFDPDLERRIALKVLRGATATTQARDRLLREARAMARLSHPNVVTVHEVGTA
                     GGRDFVAMELIHGETLAEWLRAQPRPPAAILDAFLAAGRGLAAAHAAGIVHRDFKPHN
                     VLRSRDGRIVVTDFGLAREAHGAPAPALDATLPLGAEITAGGSSSPLAGLTVTGSLLG
                     TPAYMAPEQWSGGAVTPATDQFAFCVALWEALAGQRPYPGPTLD"
     gene            complement(834..1553)
                     /locus_tag="VF516_36835"
     CDS             complement(834..1553)
                     /locus_tag="VF516_36835"
                     /inference="COORDINATES: protein motif:HMM:NF025871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing redox enzyme family protein"
                     /protein_id="HEX8113372.1"
                     /translation="MDPQTFREALLQIMERKTHWAWPQFTSGKVAKDRLHLHFEQEYG
                     TYVRDFPVMLGWAYVQCPIPEVRHDLAENLYEEETGGLIAGRPHPELFLEYPRGLGMD
                     LSRFERVELGPAAQRFRALLDDYTQRRGWAQACAVTTIFLEGTDHERGELDRTAPRRP
                     EPPLELHPLVVHYGLPLDALALTKAHRRVEGSHRAAAWRIILDHVPDTEHAGVVIAME
                     ATLAAWHAYRDEVAELCGLRP"
     gene            complement(1731..2270)
                     /locus_tag="VF516_36840"
     CDS             complement(1731..2270)
                     /locus_tag="VF516_36840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113373.1"
                     /translation="MSGPIRTADAVSAPVRTADAASAPARTADAVSAPVGTADPMSAP
                     VRTAEATSPPAEHLDRITNRASAPADSTALYRKRTNPALPTVSPDVIAGAQPVTDTPE
                     VDAAIDAAIDAAIGQGPPPPGPDDAPSALPSVMLDAPASPGLGMRPLALPRPPMQLIL
                     SIIAAALVVIGLWMLHLAF"
BASE COUNT          325 a          842 c          994 g          332 t
ORIGIN      
        1 cgtcgagcgt gggtccggga tacggacgct ggccggcgag cgcctcccac agcgcgacgc
       61 agaatgcgaa ctgatcggtg gcgggggtga cggcgccgcc gctccattgc tcgggagcca
      121 tgtaggcggg ggtgccgagc agcgagccgg tgacggtgag cccggcgagc gggctggacg
      181 aaccgcccgc ggtgatctcg gcgccgagcg gcagggtggc gtcgagcgcg ggagccggcg
      241 caccgtgggc ctcgcgggcg aggccgaagt cggtgacgac gatccggccg tcgcggctgc
      301 gcaggacgtt gtggggcttg aagtcgcggt ggacgatgcc ggcggcgtgg gcggcggcga
      361 ggccgcggcc ggcggcgagg aaggcgtcga ggatcgcggc gggcggtcgg ggctgggcgc
      421 gcagccactc ggcgagggtc tcgccgtgga tcagctccat ggcgacgaag tcgcggccgc
      481 cggcggtgcc gacctcgtgg acggtgacga cgttgggatg cgacaggcgg gccatggcgc
      541 gggcctcgcg cagcaggcga tcgcgggcct gggtggtcgc ggtggcgccg cgcaggacct
      601 tgagcgcgat ccggcgctcg aggtcggggt cgaacgcggc gtggacgacg cccatcgcgc
      661 cggcgccgag ctcgcgctcc aggcgatagc gacccaggtt tgcgccgaac ggttcggccg
      721 gttccgctgg tgcgctggcc gccgccgtcg cggtccgcgc gagctcctgc tctcgatcac
      781 tccccacgcc ccgaccatag cgcaggatcg gcccggatgg cgcgctgcgt cgttcagggc
      841 cgcaggccgc agagctcggc gacctcgtcg cggtacgcgt gccaggccgc gagcgtggcc
      901 tccatcgcga tcaccacccc ggcgtgctcg gtgtccggga cgtgatcgag gatgatgcgc
      961 cacgcggcgg cgcggtggct gccctcgacg cggcggtgcg ccttggtcag cgccagcgcg
     1021 tcgagcggca ggccgtagtg cacgaccagc gggtggagct cgagcggcgg ctcgggccgg
     1081 cgcggcgcgg tgcggtcgag ctcgccgcgc tcgtggtcgg tgccctcgag gaagatcgtg
     1141 gtcaccgcgc aggcctgcgc ccagccgcgg cgctgggtgt agtcgtcgag cagcgcgcga
     1201 aaccgctgcg cggcggggcc gagctcgacg cgctcgaacc gggacaggtc catgccgagg
     1261 ccgcgcgggt actccaggaa cagctcgggg tgggggcggc cggcgatcag gccgccggtc
     1321 tcctcctcgt agaggttctc ggcgagatcg tgccggacct cggggatcgg gcactgcacg
     1381 taggcccacc cgagcatcac ggggaagtcg cggacgtagg tcccgtactc ctgctcgaag
     1441 tggagatgca gccgatcctt ggcgaccttt cccgacgtga actgcggcca cgcccagtgc
     1501 gtcttgcgct ccatgatctg aagcaacgcc tcccggaagg tctggggatc cacggtagga
     1561 gcctacgcgt gcgtcgcccg acgagcaggc gcggcatggc tcgacgccag cgtcacgttg
     1621 ccgcacccag atcgttgcgc ggtgcgcgca aacgccgtgc gtgacgctca ccacacggcg
     1681 gacgcccaca tggcggatgg ctctccggcg caggtgctgg gcctcggcgc tcagaacgcg
     1741 aggtgcagca tccacagccc gatcaccacc agcgcggcgg cgatgatcga gaggatcagc
     1801 tgcatcggcg ggcggggcag cgcgagcggg cgcatcccga ggccgggcga cgcgggcgcg
     1861 tcgagcatga ccgacggcag ggcggagggc gcgtcgtccg ggcccggcgg aggcgggccc
     1921 tgaccgatcg ccgcatcgat cgcggcatcg atcgccgcgt cgacctcggg agtatcggtg
     1981 accggctggg cgccggcgat cacgtcgggc gacacggtcg gcagcgccgg gttggtgcgc
     2041 ttgcgataca gcgcggtcga atccgcaggt gcgctcgccc ggttcgtgat gcgatcgagg
     2101 tgttcggcgg gcgggctggt ggcctcggcg gtgcggaccg gtgcgctcat cggatcggcg
     2161 gtaccgaccg gcgcgctcac cgcgtcggcg gtgcgggccg gcgcgctcgc cgcatcggcg
     2221 gtgcggaccg gagcgctcac cgcgtcggcg gtgcggatcg gtccgctcac gccgtcgcgc
     2281 cgcatcgtgt gcgcgtagcg acgcgattga cgatctcccg tcacacccgg ctcgatgaac
     2341 tcgggcacgc cgtcgatgcg accagtttcg ccggatgtgc ccgcggaacg cggctcattc
     2401 cgtcggatgg gactacctgc aaccccctgc ggcacggtct ggtcagccat cggccgcacg
     2461 ttagcagcgc ccggtgcgca tcgaaacgac ggc
//
LOCUS       DASVOR010001985         5230 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_341891,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001985 DASVOR010000000
VERSION     DASVOR010001985.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5230
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341891"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..326
                     /locus_tag="VF516_36845"
     CDS             <1..326
                     /locus_tag="VF516_36845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113374.1"
                     /translation="LAAVLLAQGRASEVLATAAEVMARYAALGAFGFRGAFVRRVHAE
                     ALEATGDHAGACRAIATARDCLRVQAAKIGDPALRRAFLEALPDNARTFALARQWLGD
                     GPASG"
     gene            complement(343..795)
                     /locus_tag="VF516_36850"
     CDS             complement(343..795)
                     /locus_tag="VF516_36850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113375.1"
                     /translation="MTTAKLREIVAKAIANPDAYPDLEVSYLRGHDLSGTTHFTVDAA
                     GDFKLESSVTRDEQTHSWSGKLPVDDRNAFLRAIESTRLLDVPSSTRNIGDDEEPIIV
                     TVREGKLVHEVRVWHDDAAAAGLAPFEAHVLTLAKRVSAGEVLTIPAR"
     gene            complement(915..2087)
                     /locus_tag="VF516_36855"
     CDS             complement(915..2087)
                     /locus_tag="VF516_36855"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8113376.1"
                     /translation="MRQEKGQDIGSDRRPDTSVQRKATASLGKSTLVEQVYGDAVQRR
                     AVSGGDGAESAHVHADAERGVSGAGAPLPHLDRIQRLFGPRHDLSGVQAHTGGEAASA
                     CDAIGASAYATGHHVAFAGAPSLHTAAHEAAHVVQQRGGVQLKGGVGQAGDAYEQHAD
                     AVADRVVAGESAEPLLDAMSGGGTQAAVQRSQPISETTDTGNRYTQELEVLPTKVQIQ
                     LAIEWGKQGTWASDADFQRFVRQCKTTVYGYLDNKFKIVGTPKPGSGAQAWERPIDFL
                     LYDNTGYKIDVFGGTQGGCSMAQGGGKIYEQTVTGAPQDPVTIAHEFGHALLGQSDEY
                     ANPAVPGRVISNDHSIMGNYKTQGRAQAEFKVRHFQHILAAVAPQFPNHTCTLAQV"
     gene            complement(2458..4614)
                     /locus_tag="VF516_36860"
     CDS             complement(2458..4614)
                     /locus_tag="VF516_36860"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113377.1"
                     /translation="MILTVIQAEFIAARRVLRIDDGCQEKDPSDGTVYLRGTVRSKLA
                     NRSYSIVLTCIGGAGNPGAAAATASAIAKCHPKAVLLMGIAAGIRDKVRIGEVVLSDR
                     VVAYEPAALIQSASGASVQPRPEIDRVPHTMSQDVVSYHTKQSRLRDAFKRAGGVIPA
                     APAGREDEFRVHVANEIALHSGTIASGEKLLRDPRKLLEVRELQHGKVEVGEMEAAGV
                     VEACRHGAVPWLVIRGISDFGDQFKDDRFHEFASCAAAAVLYDFVAYGLNLSTASSPS
                     PGHDDATALPSATRKRFVSWLGLLISAIGLVCVATVLLTVVWRGHRSSPSPPICEDGI
                     EFVRLPGGTFESGNAAERAPRRQLTVSAFYASRYALPSPMAIGSSCSPPPQMPPIGQW
                     MLAAEFCERQNARLITEAEWEFLAHKSPENLEPGAREFTWDCYFNRSDGGRVPSVNPT
                     LHIDGCFLHTARGLAADNSDIEIRYSNPEDGRSRCVWSNGLDPDGRVYRWLILGPAAW
                     GGPDTPFPEMPKLLSGSPRLGDYSEWRLGNKTGEGRWLAYEPHFENKRSTSGCEPRDR
                     ACLNHKYYSADIGCFLFKSANFSGGHAAFLQSYLVVPEMRRVRFSAAADDNFQLWLNG
                     EPIAKYIGPPTCLDEFDEKNEPRVTWSNTDVLELQQGVNRLTAVVGGIGNSWLFSVRI
                     FSENNAPIGEQIFATLDPDDISIALPLNPIKVPPLQ"
BASE COUNT          912 a         1670 c         1680 g          968 t
ORIGIN      
        1 cgctcgccgc cgtgctgctc gcgcagggcc gcgcttccga ggtgctcgcc accgccgcgg
       61 aggtcatggc gcggtacgcc gcgctgggcg ccttcgggtt tcgcggtgcg ttcgtccggc
      121 gggttcacgc cgaggcgctg gaggccaccg gagatcatgc cggcgcctgc cgggccatcg
      181 ccacggcgcg cgactgcctg cgggtccagg cggcgaagat cggggacccg gccctgcgcc
      241 gggccttcct cgaagccctg cccgacaacg cccgaacgtt cgcgctcgca cggcaatggc
      301 tgggcgacgg tcccgcgtcg ggctgacgcc gggagcgaga cgctaccgcg cggggatggt
      361 caggacctcg ccggcggaga cgcgcttggc gagcgtcagc acgtgcgcct cgaagggcgc
      421 gaggccggcc gcggccgcgt cgtcgtgcca gacccggacc tcgtgcacga gctttccctc
      481 gcggacggtg acgatgatgg gctcctcgtc gtcaccgatg ttgcgggtcg acgaggggac
      541 gtcgaggagc cgggtcgatt cgatcgcgcg caggaacgcg ttgcgatcgt cgacgggcag
      601 cttgcccgac cacgagtggg tttgctcgtc ccgggtgacg ctcgattcga gcttgaagtc
      661 gccggccgcg tccaccgtga agtgggtggt gccggacaga tcgtggcctc gcaggtacga
      721 gacctcgaga tccgggtagg cgtcggggtt cgcgatcgcc ttggcgacga tctcgcgcag
      781 cttggcagtc gtcatggtct gatcttgcct ctggtcggaa tgggctggct tgatatcgcg
      841 cgcgtcggtg gctggccggc gaccacagcc gaggctcgcc accaggacca cggccagcag
      901 gtcgcggcgc acgttcatac ctgcgccagg gtgcaagtgt ggttggggaa ctgcggcgcg
      961 accgccgcga ggatgtgctg gaagtgccgc accttgaact cggcctgagc acggccctgc
     1021 gtcttgtagt tgcccatgat gctgtggtcg ttcgagatca cacgaccggg cacggccggg
     1081 ttcgcgtatt cgtcgctctg gccgagcagg gcgtgcccga actcgtgcgc gatggtcacc
     1141 ggatcttgcg gcgcgccggt gaccgtctgc tcgtagatct tgcctccgcc ctgggccatc
     1201 gagcagccgc cctgggtccc gccgaagacg tcgatcttgt agcccgtgtt gtcgtacagc
     1261 aggaagtcga tcggtcgctc ccacgcctgc gcgcccgacc cgggcttggg cgtgccgacg
     1321 atcttgaact tgttgtcgag gtagccgtag accgtggtct tgcactgacg gacgaacctc
     1381 tggaaatccg catcgctggc ccaggtcccc tgctttcccc actcgatggc gagctggatc
     1441 tggaccttgg tggggaggac ctcgagctcc tgcgtgtacc ggttgccggt atcggtggtc
     1501 tcgctgatcg gctggctgcg ctggaccgcc gcttgcgtcc cgccgcccga catcgcgtcg
     1561 agcaggggtt cggccgactc gcccgccacc acccggtcgg cgaccgcgtc ggcgtgctgc
     1621 tcgtaggcgt cgccggcttg accgaccccg cccttgagct gcacaccgcc gcgctgctgg
     1681 acgacatggg ccgcctcgtg ggccgcggtg tgcaggctgg gcgctccggc aaacgcgacg
     1741 tggtggccgg tcgcgtacgc gctggcgccg atcgcatcgc atgcactcgc cgcctcgccg
     1801 ccggtgtgcg cctgcacgcc agacagatcg tggcgcggtc cgaacagtcg ctggatcctg
     1861 tcgagatggg gcagcggcgc ccccgccccg gacaccccgc gctcggcatc ggcgtgaacg
     1921 tgcgccgact cggcgccgtc gccacccgaa actgcgcgcc gttgcaccgc gtcgccgtac
     1981 acctgctcga ccagcgtgga cttgcctagg gaggcggtgg ccttgcgctg gaccgaagta
     2041 tcggggcgcc gatcgctgcc gatgtcctgc cccttctctt gccgcatgtc cttccctccg
     2101 ggcctggtgt gaagcatacc acgagcgccg gacgtgatcc ttccgcgcgt ggtggtcccg
     2161 gcttacgcga cgcagggcaa gcgcctggcc aatgacctcc caggtcgcag gtgcgccgag
     2221 ttctcacata cagaatgtcg aggtagacgg cgtcggatgc ggaaggtgct gcgccgatgg
     2281 agtaccagta gcgagcgaca ctgggcggca cctcgaagct gcggcgatcc ggtggatcga
     2341 gtagagacca ccggctcggg gctcagcgtg tgacacgagt cgtgcggttg gccgagccgt
     2401 gatcgtagcg gatggctgcg ccatccgtaa ctcgcggcgg tttagctccg cgacccctca
     2461 ttggaggggc ggaaccttga tcgggtttag gggcagcgca atagatatat cgtccggatc
     2521 gagcgtggcg aagatctgct ctccgatggg agcattgttc tcggagaaga tccgcaccga
     2581 aaatagccat gagtttccta tgcctccgac cacggcggtc aagcgattga ctccttgctg
     2641 tagctctaac acgtctgtgt ttgaccacgt cactctaggc tcattttttt cgtcgaattc
     2701 atcgaggcac gtgggtgggc caatgtactt ggcgatcggc tcaccattca accaaagttg
     2761 aaaattatcg tccgcagccg cggagaatcg gactcgacgc atctcgggaa caacaagata
     2821 cgattgcaga aaagctgcat ggcctcccga aaagtttgcc gacttgaaga gaaaacaacc
     2881 gatatctgca ctgtagtatt tgtgatttag acacgcccga tccctcggtt cgcatccgga
     2941 ggtgcttcgc ttgttctcaa agtgtggctc gtaggctagc caccgacctt ccccagtctt
     3001 attaccgagc cgccattcgg agtagtctcc aagtctaggg ctgccagaga gcagcttggg
     3061 catttcaggg aacggtgtat cggggccacc ccacgcggcc ggtcccaaga ttaaccagcg
     3121 gtatactctt ccatcggggt ctaatccatt agaccaaacg catctagaac ggccgtcttc
     3181 tgggtttgaa tatcgaatct cgatgtcgct gttgtcggct gcaagccctc gtgcggtatg
     3241 aaggaaacaa ccgtctatat gaagcgtcgg gttgaccgaa ggaactcgac ctccatcgga
     3301 ccgattgaaa taacagtccc atgtaaattc gcgggctcca ggctctaaat tttcgggcga
     3361 cttatgcgcg agaaattccc actccgcctc ggtgattaag cgtgcattct ggcgttcaca
     3421 gaactccgct gctaacatcc actgaccaat aggtggcatt tggggcggcg gcgaacagga
     3481 gctgccgatg gccatgggag acggcaatgc atacctagaa gcgtaaaatg cgctcacggt
     3541 tagttgcctt cggggagcgc gttctgctgc atttccggac tcgaacgtcc caccaggtaa
     3601 tcgaacaaac tcaatgccat cctcgcatat cggaggtgat ggcgaagatc gatggcctcg
     3661 ccatacaacc gtgagcagca cggtcgcaac acaaactaac ccgatcgcgc taatcaagag
     3721 acccaaccag gagacgaaac gttttcgagt cgcagacggc agagcggtgg cgtcgtcgtg
     3781 cccaggagaa ggggacgacg cagtgctgag attgaggccg tacgcgacga agtcgtacag
     3841 aaccgccgcc gccgcacacg acgcgaactc gtgaaaccga tcgtctttaa actggtcgcc
     3901 aaagtcagag atccctctaa tcaccagcca tggcacggcc ccgtgccgac acgcttctac
     3961 gacgccggcc gcttccattt cccctacctc gaccttgccg tgttgcagtt cgcgcacttc
     4021 aagcagcttc cttggatcgc ggagcagctt ctctccgctc gcgatggttc ccgagtgtaa
     4081 ggcaatctcg ttcgccacgt gaacgcggaa ctcgtcctcc cgaccagcgg gtgcggccgg
     4141 aatgacgccg ccagcgcgtt tgaacgcatc acgcaggcgc gattgcttcg tgtggtagct
     4201 caccacatcc tggctcatag tgtgggggac acgatcgatc tcaggccggg gttgcacgct
     4261 cgcgccgctc gccgactgaa tcaacgccgc gggctcgtag gccaccacgc gatccgagag
     4321 cacgacctcg ccgatccgga ccttgtcgcg gatgcccgcc gcaatcccca tgagaagcac
     4381 ggccttcggg tggcacttcg cgatggcgct agcggttgcc gccgcagctc cagggttacc
     4441 tgcaccaccg atgcaggtca gcacgataga atagctccgg ttggctagct tagaacgcac
     4501 cgtcccccgg aggtagaccg ttccatcaga agggtccttc tcttggcagc catcgtcgat
     4561 ccgcagtacc cgacgcgccg ctatgaactc ggcttggatg accgtcagga tcacgacgtc
     4621 gactggcttg cccagttctt gccctgatct caaacgcgcc atatgctaca ttgaagacga
     4681 ccgcatcgat ctgtgcaagt aacgtccgtg atgactgatg ccaggcagcc cgttcagcgg
     4741 gcagggcagc gccgaagatg tcactggtgc ctgcgaagct gtcccaccgg cgtcaaggag
     4801 cccatttcca ctgggtcacc ggtacccatt ccgaagcaat cgaaccggct gtttctgcag
     4861 agatagaacc caccgtggtg gtcggcagag tggtttgttc gggcgctctg tagagccatc
     4921 accacccaga tgcaagcgcc cagaggccat agggctggtc cctaccgagc cggcccccag
     4981 ggccgaaagg tagggatcgc ggtagggacc ggcgcggtag ggaccggcgc gagcggcctg
     5041 acattgtccg gcgtctggct ggatcttggg tgacctccga ggagcttgga ccattcgaga
     5101 tcgactggct ccggcacgcc cggaagtcag atcgccgcgg cgcacgcctc ggcgatgaac
     5161 agatcctcga cgccgagccc ggtgaagtcc gcgaccgtga tcccggcgcg gtcgtacgcg
     5221 acgggggcgg
//
LOCUS       DASVOR010001986         1857 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_282939,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001986 DASVOR010000000
VERSION     DASVOR010001986.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282939"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VF516_36865"
     CDS             complement(<1..863)
                     /locus_tag="VF516_36865"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8113378.1"
                     /translation="MLYNLHLLRVIAALGVVYFHTTSVAGLKLDWDVGSRGVDVFFVI
                     SGFIIAYIGTSKPEQFFLRRLIRVVPFYWAATAFVFAMVSIAPNLFHTTTASVPHLIA
                     SLLFFPRPGADGEMQPTLILGWSLNYEMFFYVWFAISLRISQRWSPVLCAGWLIAVML
                     AIDVARSANPVAAFYARPIVLEFCYGLVAFYVFQWCSARRDALARVSGLQWALLAILV
                     GGLVAIAVGEEYYRDVVPRYLIAGIPSFFVVTSALLLERLFGLSTKNRLIFLLGEASY
                     IIYLVHPYIIFS"
     gene            1097..>1857
                     /locus_tag="VF516_36870"
     CDS             1097..>1857
                     /locus_tag="VF516_36870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113379.1"
                     /translation="MRTALRTTWWIGLACWLWAWAAPVEAAPPEPSGPHPRMLLDGEL
                     RAAWRARGKEGHGPIGGAIAVCGGDRSPREHAAALYMGAEWKKMLQACLVAWAATGEP
                     DHARTALRYFTALIDDRDTIGDGKGGDEAANRDAGYAIRNLGPYTALAYDWLHDAPGM
                     TPELRARARRRWAAWLAWFERSGYHPHDPGSNYHAGYLLAATMIAIAEAGEAGDAGSA
                     LWRDVADRMWGKEMAAAWAPGGPLDGGDWDEGWQYG"
BASE COUNT          318 a          631 c          685 g          223 t
ORIGIN      
        1 gagaagatga tgtacgggtg gacgaggtag atgatgtacg acgcctcgcc gagcaggaag
       61 atcagccggt tcttggtcga caggccgaac aaccgctcga gcagcagcgc gctggtcacc
      121 acgaagaacg atgggatccc cgcgatcagg taacggggca cgacgtcgcg gtagtactcc
      181 tcgcccacgg cgatcgccac caggccgccg accaggatcg cgagcagcgc ccactggagg
      241 cccgagaccc gggcgagcgc gtcgcgccgc gcgctgcacc actggaacac atagaacgcg
      301 accaggccgt agcagaactc gagcacgatc ggccgggcgt agaacgccgc caccgggttg
      361 gcgctgcgcg cgacgtcgat cgcgagcatc accgcgatca gccagcccgc acacaatacc
      421 ggcgaccagc gctgcgagat ccgcagcgag atcgcgaacc acacgtagaa gaacatctcg
      481 tagttcagcg accagccgag gatcagcgtc ggctgcatct cgccgtcggc gcccgggcgg
      541 gggaagaaca gcagcgacgc gatcaggtgc ggcacgctcg ccgtcgtggt gtggaacagg
      601 ttgggcgcga tcgacaccat cgcgaacacg aacgcggtgg ccgcccagta gaacggcacg
      661 acgcggatca gccggcgcag gaagaactgc tcgggcttgc tcgtgccgat gtacgcgatg
      721 atgaagccgc tgatcacgaa gaacacgtcg accccgcgac tgccgacgtc ccagtcgagc
      781 ttgagcccgg cgaccgaggt ggtgtggaag tagacgacgc cgagcgccgc gatcacgcgc
      841 agcaggtgca ggttgtagag catgtgaatg aacgctcgcc cccatcgtcc cgtgccggca
      901 cacggcaccg caagtgaccg gcggtgcaat cttccactgc cgctcaccgc cgttgtgaga
      961 ctggcctgac cgcttcggcg aaccgccgcg gcgagggtcg tgagtggggc gctttcgcac
     1021 ggtgccgtgc gatggcgctc gctcggcgcc ggcgcggccg ggcgtgtgcg ggcataccgg
     1081 atgctacgtt tcccgggtgc gcactgcatt gcgaacgacc tggtggatcg gccttgcatg
     1141 ctggctgtgg gcgtgggcgg cgccggtgga ggcagcaccg cccgagccga gcggtccgca
     1201 tccccgcatg ctgctcgatg gcgagctgcg cgcggcgtgg cgggcgcgcg gcaaggaggg
     1261 ccacggcccg atcggcggcg cgatcgccgt gtgcggcggc gatcgcagcc cgcgcgagca
     1321 cgccgccgcg ctgtacatgg gcgcggagtg gaagaagatg ctgcaggcct gcctggtcgc
     1381 gtgggcggcg accggcgagc cggaccacgc ccggaccgcg ctgcgctact tcaccgcgct
     1441 gatcgacgac cgcgacacca tcggcgacgg caagggcggc gacgaggccg ccaaccgcga
     1501 cgccggctac gcgatccgca acctcgggcc gtacaccgcg ctggcctacg actggctgca
     1561 cgacgcgccc ggcatgacgc ccgagctgcg cgcccgcgcc cgccggcggt gggcggcctg
     1621 gctggcgtgg ttcgagcgca gcggctacca cccgcacgat ccgggcagca actaccacgc
     1681 cggctacctg ctcgcggcga cgatgatcgc gatcgccgag gccggcgagg cgggcgacgc
     1741 cggcagcgcg ctgtggcgcg acgtcgccga ccggatgtgg ggcaaggaga tggccgccgc
     1801 gtgggcgccc ggcgggccgc tcgacggcgg cgactgggac gagggctggc agtacgg
//
LOCUS       DASVOR010001987         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_109529,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001987 DASVOR010000000
VERSION     DASVOR010001987.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109529"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..442
                     /locus_tag="VF516_36875"
     CDS             <1..442
                     /locus_tag="VF516_36875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113380.1"
                     /translation="LALSGAGFAGYRLHGGPGAARMNEPEPSQTAQGPVSATGERLRR
                     IARDDGELIVRALVPEAIRASVPVLTHLEIKTKLGAEFNATQVVITIADPDGNASGQT
                     AVMHGDEAGHYMFRHTFARPGRHIVRIFPSDTEAMSTFELDVEP"
     gene            complement(448..993)
                     /locus_tag="VF516_36880"
     CDS             complement(448..993)
                     /locus_tag="VF516_36880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113381.1"
                     /translation="MKLRSVLVASFAVLVLALGVRSAAAENPNQVFSGRIMMSSKRFP
                     QSASSGSAYVAAIRKQSQTAFAEAKDTHTWKIYFAGFLKVPLNDLEYAIKLYDVSGKG
                     QQLLLTFDQFTDERGQKTIVSNMTLDKKQVGVNKELLMVMESKGKVLASSRFKILGEG
                     EHFTGKVNFTEDEASGKKTDD"
     gene            complement(1111..>1917)
                     /locus_tag="VF516_36885"
     CDS             complement(1111..>1917)
                     /locus_tag="VF516_36885"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HEX8113382.1"
                     /translation="RLAGSTAGVAAFSSAPPDLLLETEVVILAVRDQVITEVAQMLLG
                     TGLINKRHVLLHCAGAAPARELLGPVASQVAGIGTLHPLSAIADGTASMRALKGTVFG
                     VEGDEVSRATAAKLVGALNGVVLALDSSQMAAYHAAAALASNYIVAAIDAAAAVLAGA
                     GVPVERAAQALVPLAEGALRNIAAHGTTGGLTGPVRRGDLATVSRHLEAIRDRPELTE
                     IYRVLACRAVEIASRIDGRDAPDRTSLEAIRALLCHRTPGTPGTPGTQGT"
BASE COUNT          286 a          669 c          675 g          287 t
ORIGIN      
        1 gcttgccctg tcgggggcag ggttcgcggg ctaccggctc cacggcggcc ccggcgcggc
       61 ccgcatgaac gagcccgagc cctcgcagac cgcacagggc cccgtgtccg ccacaggcga
      121 gcggctgcgg cggatcgcgc gcgacgacgg cgagctgatc gtccgcgccc tggtccccga
      181 ggcgatccgc gccagtgtgc cggtcctcac ccacctggag atcaagacca agctcggcgc
      241 cgagttcaac gccacccagg tcgtgatcac gatcgccgat ccggacggca acgcgtccgg
      301 acagaccgcc gtgatgcacg gcgacgaggc gggccactac atgttccgcc acaccttcgc
      361 ccgccccggc cgccacatcg tgcggatctt cccatcggac accgaggcga tgtcgacgtt
      421 cgagctcgac gtggagccct gactctacta gtcgtcggtc ttcttgccgc tcgcttcgtc
      481 ctcggtgaag ttgaccttgc cggtgaaatg ctcgccctcg ccgaggatct tgaaccggct
      541 ggaggccagg accttgccct tgctctccat gaccatcagc agctccttgt tgacgccgac
      601 ctgcttcttg tcgagcgtca tgttcgacac gatggtcttc tggccgcgct cgtcggtgaa
      661 ctggtcgaac gtcagcagca gctgctggcc cttgccgctg acatcgtaga gcttgatcgc
      721 gtactcgagg tcgttgagcg gcaccttgag gaagccggcg aagtagatct tccaggtgtg
      781 ggtgtccttg gcctcggcga aggcggtctg ggactgcttg cggatcgcgg cgacgtaggc
      841 cgagccgctc gacgccgact gcgggaaccg cttgctgctc atcatgatgc ggccggagaa
      901 cacctggttg gggttctcgg cggcggcgga ccggaccccg agcgcgagca cgaggaccgc
      961 gaaggacgcg acgaggacgg agcgcagctt catcgcggcg atcctagcgg accgatcgtg
     1021 taggagtcga ccgctccgcg gggccgggcc atcaggtcac cgtcagggga tccaaagcca
     1081 ccgtcagaaa gccgaccggg atggacgccg ttacgtcccc tgcgtccccg gcgtacccgg
     1141 tgtgcccggt gtgcgatgac acagcaacgc gcggatcgcc tcgaggctcg tgcgatccgg
     1201 cgcgtcgcgg ccgtcgatcc gggacgcgat ctcgaccgca cggcaggcaa gaacccggta
     1261 gatctcggtg agttccggcc gatcgcggat tgcctcgagg tggcggctca cggtcgcgag
     1321 atcgccgcgg cgcaccggcc cggtcagccc tccggtggtg ccgtgtgcgg cgatgttgcg
     1381 caatgcgccc tcggcgagcg ggaccagcgc ctgcgccgcg cgctcgacgg ggaccccggc
     1441 acccgcgagc accgcggccg ccgcgtcgat cgcagcgacg atgtagttcg aggccagcgc
     1501 cgcggccgcg tggtaggcgg ccatctgcga gctgtcgagc gccagcacga cgccgttgag
     1561 cgcgccgacc agcttggccg cggtcgcgcg gctgacctca tcgccctcga cgccgaacac
     1621 cgtgcccttg agcgcgcgca tcgaggccgt gccatcggcg atcgccgaca gcgggtgcag
     1681 cgtcccgatc ccggcgacct gcgacgcgac cgggccgagc agctcccggg ccggcgcggc
     1741 gccggcgcag tgcagcagca cgtggcgctt gttgatcagg ccggtgccga gcagcatctg
     1801 cgcgacctcg gtgatgacct gatcgcgcac ggcgaggatc acgacctcgg tctcgaggag
     1861 cagatcgggc ggggccgagg agaatgcggc gacgccggcg gtggagccgg ccaggcg
//
LOCUS       DASVOR010001988         4679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_196467,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001988 DASVOR010000000
VERSION     DASVOR010001988.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196467"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1015
                     /locus_tag="VF516_36890"
     CDS             <1..1015
                     /locus_tag="VF516_36890"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113383.1"
                     /translation="LYRLQRARNALEDLDTALAIARDLGDARLEVEVLLEHAAALDWS
                     DDYAGSTAFAERAFARHREARIADMALDIALARARIAYRQARLDEAIPVLDEVVALAR
                     RDGGAETETIALVMLGGMLAQARQFDRAEVVFRELIALCERRDDRFHLGAAYSNRIVL
                     WTSLGQAERAEADLRTVIQLARETGLAALERGATHNLAEQRLWQGALDEALSLARRGL
                     SLQRGHGEGTTWVDELLLARILAARDDLAEVRGLLGAIAPHDIGDDNRIIVGVLQACV
                     APSAPAVWREWLARADTVLDDDVRLELALLAHRHRALSPEKLAEARALAQRHPVWSRR
                     HAF"
     gene            1149..2312
                     /locus_tag="VF516_36895"
     CDS             1149..2312
                     /locus_tag="VF516_36895"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113384.1"
                     /translation="MDGSQPLVIPGHQLDRLIGEGGFGQVWHARRDADGEAVAIKVLH
                     LELVRSVDAMTRFQRELDAVERLDHPNVVRGLGHGTLPDGRPYLILEYVEGPSLREVL
                     HERGGLPPAEMLQILEPLCDALHTAHEAGLVHRDVKASNVILARRGGAIRPVLLDFGL
                     VKLVDEVGPGLTSSRSMLGTPAAMAPEQMRGQPVDPRTDVYALGLLAYHVLTGQPAFG
                     GAPGVVQSYLQVHGPRPRPSAKVDIDPAIDEPVARALSPEPADRFADARSFAEALRAV
                     IHGGVGEVQEVAVLYVEGDPADIESATAAARALGLTVALTAPDSVLAVVPRKRVDIAA
                     LRSRVSAFSVHARVAIGISHATVSGDLVDGPALDVESWAPYPLPTGLWVADGL"
     gene            2499..3272
                     /locus_tag="VF516_36900"
     CDS             2499..3272
                     /locus_tag="VF516_36900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113385.1"
                     /translation="MVSSNRILLNRILLNRIALEQIALDRISVGRLSVNTASAGGLLA
                     TADGRDVFSLVVSCAVPSDVTLVATVNGAQFEFPGEVGLAPQWLSTRLAHPGQRWVSA
                     CMFARVNAHEVVIPISMRGPHPELATSAAERAAWTLEEGAFFGNAFGPVARPLQAFAC
                     RGKDKAAGNTGELADRDCAAPDPANPGFTLCGMMFAGDCGSFAADQACESFSVRGTFY
                     EDCHTSPIRSYHGGGQAHGQDQDDDERVFAQVITTFITP"
     gene            3392..4246
                     /locus_tag="VF516_36905"
     CDS             3392..4246
                     /locus_tag="VF516_36905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113386.1"
                     /translation="MAGTSCTTWCQETSPTANRLHGVWAASASDVFAVGDGGKILRRV
                     NGTWTQMTSPTTNNLRGVWGLSSSDVWASGVAVNGVGTVLHFNGTAWSLVPGATTDLE
                     SVWAASSTDVWFAGSTVVLRWNGSAFSNPGNFAGPLLSVSGTSSNDVWVTGENTNVHH
                     WNGSAWTTFMPPVGTTSFLCVLAVAVNDVWVSDFMPGKETAHWTGGSTTKWATAAASA
                     GGWNGMSALASNDIWGAGGSHAGHWNGSAWSQETPPGTNPILWSVTTRPGNAWVVGDN
                     GLIDHRTF"
     gene            4336..>4679
                     /locus_tag="VF516_36910"
     CDS             4336..>4679
                     /locus_tag="VF516_36910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113387.1"
                     /translation="MRRLAGCLVVSLLASAAAWVGCRDDLADEPPAFAVGSGTDAGMG
                     GDAYGGGTVGLDAIVVIDDAIVLADGSGEGGLPDAGSGMVDAGSGEGGVPDAGSDAGG
                     GGGTDDGGGTDDG"
BASE COUNT          667 a         1582 c         1721 g          709 t
ORIGIN      
        1 gctgtaccgc ctgcagcgcg cgcgcaacgc gctggaggat ctcgacaccg cgctggcgat
       61 cgcgcgcgat ctgggcgatg cgcggctcga ggtcgaggtg ctgctggagc acgcggccgc
      121 gctcgactgg agcgacgact acgccgggtc gaccgcgttc gccgagcgcg cgttcgctcg
      181 ccaccgcgag gcccggatcg cggacatggc gctcgacatc gcgctggccc gggcgcggat
      241 cgcgtaccgc caggcgcggc tcgacgaggc gatcccggtg ctcgacgagg tcgtcgcgct
      301 ggcccgccgc gacggcggcg ccgagaccga gaccatcgcg ctggtcatgc tcggggggat
      361 gctcgctcag gccaggcagt tcgaccgcgc cgaggtcgtg ttccgcgagc tgatcgcgct
      421 gtgcgagcgc cgcgacgacc gcttccacct cggcgcggcc tactcgaacc gcatcgtgct
      481 gtggacgagc ctcggccagg ccgagcgcgc cgaggcggac ctgcggacgg tgatccagct
      541 cgcgcgcgag accgggctgg cggcgctcga gcgcggcgcg acccacaacc tcgccgagca
      601 gcggctgtgg cagggcgcgc tcgacgaggc gctcagcctg gcccgccgcg gactgtcgct
      661 gcagcgcggc cacggcgagg gcacgacctg ggtcgacgag ctgctgctcg cgcggatcct
      721 cgcggcgcgc gacgatctcg cggaggtgcg cggcctgctc ggcgcgatcg ccccgcacga
      781 catcggcgac gacaaccgga tcatcgtcgg cgtcctgcag gcgtgcgtcg cgccgagcgc
      841 gcccgcggtg tggcgcgaat ggctggcgcg cgccgatacc gtgctcgacg acgacgtccg
      901 gctcgagctc gcgctgctcg cgcatcgcca ccgcgcgctg tcaccggaga agctcgcaga
      961 ggcccgcgcg ctcgcgcagc gccacccggt ctggtcgcgg cgccacgcgt tctgagggtt
     1021 gcaccccttg cagccgcccc gcagggtgtg cgcggcggct cgaaagtcgc ggcagcgttc
     1081 ctggccccgg cgcattcgca tgcgatcgga ataggcgtgc caccggacgc gatggtaaga
     1141 gtgctctgtt ggacggctcc cagcccctcg tgattccggg acatcagctc gatcgcctga
     1201 tcggcgaagg cgggttcggg caggtgtggc acgcgcggcg cgacgccgac ggcgaggccg
     1261 tcgcgatcaa ggtgctgcac ctcgagctgg tccggtcggt cgacgcgatg acccggttcc
     1321 agcgcgagct cgatgcggtc gagcggctcg atcaccccaa cgtggtgcgc ggcctgggcc
     1381 acggcacgct gcccgacggc cggccgtacc tgatcctgga atatgtcgag gggccgagcc
     1441 tgcgcgaggt gctgcacgag cggggagggc tgcccccggc cgagatgctg cagatcctgg
     1501 agccgttgtg cgatgcgttg cacaccgccc acgaggcggg gctcgtgcac cgtgacgtca
     1561 aggcatcaaa tgtcatcctc gcccgccgcg gcggggcgat ccgtccggtc ctgctcgatt
     1621 tcggcctggt caagctcgtc gatgaagtcg ggccggggct gacctcgtcg cgcagcatgc
     1681 tcgggacgcc ggccgcgatg gcgcccgagc agatgcgcgg ccagccggtc gacccgcgca
     1741 ccgacgtcta tgcgctcggc ctgcttgcct atcacgtgct gaccggtcag cccgcattcg
     1801 gtggggctcc cggcgtcgtc cagagctacc tccaggtcca cgggccgcgg ccgcggccgt
     1861 cggcgaaggt cgacatcgat ccggcgatcg acgagccggt cgcgcgggcg ctgtcacccg
     1921 agccggccga ccggttcgcg gacgcgcgca gcttcgccga ggcgctgcgc gcggtgatcc
     1981 acggcggggt cggcgaggtc caggaggtcg cggtgctcta tgtcgagggt gacccggcag
     2041 atatcgagtc tgcgactgcg gcggcgcgcg cgctcgggtt gaccgtggca ctcacggccc
     2101 ccgacagcgt gcttgcggtc gtgccacgca agcgagtcga catcgccgca ctacgctcca
     2161 gagtctcggc gttttccgtg catgcccggg tcgcgatcgg catcagccac gcgacggtga
     2221 gcggcgatct cgtcgatggc ccggcgctcg acgtcgagag ctgggcaccg tatccgctgc
     2281 ccactggttt gtgggtcgct gacggtctct gacggtccac gacctacctg ggtaccaaga
     2341 ccacgcctca cgcaacatgg tcttgcgcgg tgatgcgtcg acagccgatc ctgcggcgca
     2401 cgggaggatg ttcgatgcgc tcagtccttt gtttattagt cgctgtatcg ctccctgcgt
     2461 gtgcgcaggc gcccgatgaa tcgtcgacga gcgcggaggt ggtcagcagc aaccggatcc
     2521 tgttgaaccg gatcctgttg aaccggatcg cactggaaca gatcgcgttg gaccggatct
     2581 cggtgggccg gctgtccgtg aacacggcga gcgccggagg tctactggca accgctgacg
     2641 ggcgcgacgt gttctcgctg gtcgtcagtt gcgcagtgcc ctcggacgtc acgctggtcg
     2701 ccacggtgaa cggggcccag ttcgagtttc cgggagaggt cgggctggcc ccgcagtggc
     2761 tgtccacccg gctcgcccac cccgggcagc gctgggtgag tgcatgtatg tttgcgcgcg
     2821 tgaatgcgca cgaggtagtc attccgatct cgatgcgcgg tcctcacccg gagctcgcga
     2881 ccagcgcggc ggagcgcgcg gcctggacgc tggaggaagg cgcgttcttc ggcaacgcgt
     2941 tcggcccggt ggccaggcca ctccaggcgt ttgcgtgccg cggcaaggac aaggccgccg
     3001 gcaacactgg tgagctcgcc gatcgcgact gcgccgcacc ggatccggca aacccgggat
     3061 ttaccctgtg cggaatgatg tttgccggcg actgcggcag ctttgccgcc gaccaggcct
     3121 gcgagtcctt ctcggtgcgg ggtacgttct acgaggactg ccacacctcc ccgatccgct
     3181 cgtaccatgg cggtggccag gcccacggcc aggatcagga cgacgacgag cgggtcttcg
     3241 cacaggtcat caccacattt ataactccgt agcaaaaaaa tgtgagatgc ttcatagcaa
     3301 tactatgaag tgtctctgct tggtgctcct cacgggctgt gtaaccgccg atctggatcg
     3361 tggagcggag ctcgagcagt ccgaatcgga ggtggctggc accagctgca cgacctggtg
     3421 tcaggagacc tcgcccacgg cgaaccggct gcacggcgtg tgggcagcga gcgccagcga
     3481 cgtgttcgcg gtgggcgacg gcggcaagat cctgcgccgg gtcaacggca cgtggaccca
     3541 gatgaccagc ccgaccacca acaacctgcg cggcgtgtgg gggttgagct cgtcggacgt
     3601 gtgggccagc ggcgtcgcgg tcaacggggt cgggacggtg ctgcacttca acggcacggc
     3661 gtggtcgctg gtccccgggg cgacgaccga cctcgagtcg gtgtgggccg cgagctcgac
     3721 cgacgtctgg ttcgcgggct cgaccgtcgt gctgcgctgg aacggctcgg cgttctccaa
     3781 cccgggcaac ttcgcggggc cgctgctctc ggtctcgggg accagctcga acgacgtgtg
     3841 ggtgaccggc gagaacacca acgtacacca ctggaacggc agcgcgtgga cgacgttcat
     3901 gccccctgtc gggaccacct cgttcctgtg cgtcctcgcg gtggccgtca acgacgtctg
     3961 ggtcagcgac ttcatgcccg gcaaggagac cgcgcactgg accggtggca gcaccaccaa
     4021 gtgggccacc gcggcggcca gcgccggcgg gtggaacggc atgtcggcgc tggcgtcgaa
     4081 cgacatctgg ggcgccggcg gcagtcacgc cggtcactgg aacggctcgg cgtggtcgca
     4141 ggagacaccg ccggggacca accccatcct gtggtctgtg accaccaggc ccgggaacgc
     4201 atgggtcgtc ggcgacaacg ggctgatcga ccatcgcacg ttctagttgc gcgccgcgtc
     4261 gccgggcgcg cctgtcatca cagtgtgcgc gaaccgacgc gcagcgcgcc gtgcgagacg
     4321 ttatgctcga cggggatgag acgactcgcc gggtgtctgg tcgtgagcct gctcgcgagc
     4381 gcggcggcct gggtcggctg ccgcgatgat ctcgcagacg agccgccggc gttcgcggtc
     4441 ggcagcggga ccgatgccgg catgggcggc gacgcctacg gcggcggcac ggtcgggctg
     4501 gatgccatcg tcgtcatcga cgacgcgatc gtcctcgccg acggcagcgg cgagggcggg
     4561 ctgcccgacg cgggcagcgg gatggtcgac gcgggcagcg gcgaaggcgg ggtccccgat
     4621 gcgggcagcg acgccggtgg cggcggcggg accgatgacg gcggtgggac cgacgacgg
//
LOCUS       DASVOR010001989         4478 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_459174,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001989 DASVOR010000000
VERSION     DASVOR010001989.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459174"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..1485
                     /locus_tag="VF516_36915"
     CDS             292..1485
                     /locus_tag="VF516_36915"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEX8113388.1"
                     /translation="MAATLIACAFIGCKKTESAGVSTGSGEPAVAAAKATAETKPADN
                     AVDPAMLAAAKTELDRAYQGTEREPPKSAAKPAPGKKVWIISPGQIGESASIATNAAK
                     EAGELIGWKMTLYDSKLDVANFSLGIRQAVAAQADGVILHSIDCPLVKQALAEARAAK
                     VKIVAYYALDCDDPSVKGQPMYDASVNFGSQFGDYAALTRAWGAAKADWVITKTKGRA
                     KVISFKEDELLVVKYIREGFEQELAKCKTCEIVKTVDFTLADLGPKLQQKAQSALLQH
                     PEANAMHVPYDSAMLLGISGALLESNRNDQIEVIAGEGFPTNLQMIRDNKGQDAANAF
                     PAQWTGYAAIDTLNSLFAGQPAQDSGIGYQMIDREHNLPPAGKGYEPKADYKAAYKKA
                     WGVAP"
     gene            1467..3008
                     /locus_tag="VF516_36920"
     CDS             1467..3008
                     /locus_tag="VF516_36920"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEX8113389.1"
                     /translation="MGSRSVVQSGTVVRVENISKTFPGTRALDRVTIELARGEIHALL
                     GNNGSGKSTLIKILAGVHRADPGGEIHAGGRRFAADHFTPDLARSAGLHFVHQNPALF
                     PMLSVAENLALGRGYETRRGRIDWRRQNARAARLIERFHIRATPEQPAVLLAPPDRTL
                     VAIARALQDQDGEHEGVLILDEPTSALAAPEVERLLSTLRGYARAGQTIVFVSHRLDE
                     VLGCSDRVSALRDGRHVGTANTRDMTETTLVEMMLGRHVEPPRVHRHGEPRSAALSVT
                     GLAGPQVSGLSFELGRGEVLGLAGLLGAGASNVLRQLFGAERRAAGELQLFGQPYAPA
                     APQDAIARGVAYVPPDRAIESSFHGMSVRANLSAVNVRRYFQGLRLRHGQEHADARAA
                     IDRFQIQLASDRQGFATLSGGNQQKVIVARWLRDQPRLLLLDEPTQGVAVHARREIHG
                     FLRDAAARGVAILVVSSDFEELAQLCDRVLVIVGGRCAGEVTADRLSAHQLTELAHFM
                     PEQSP"
     gene            3005..4030
                     /locus_tag="VF516_36925"
     CDS             3005..4030
                     /locus_tag="VF516_36925"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8113390.1"
                     /translation="MSEVLDPTATSASAPAEHAPRKPAGTRGIVFAVQRYGLALLFAL
                     VVLGFSVMMPESFATVLNFRNITANQSVLAIVTLAAIIPLIGGNFDLSIGAGLGVTSI
                     ATASLLSHGLPLWLAMLVGIALGATIGLFNGLLVAKVGVNALITTLGVATILTGLTVK
                     YTGGLSIITGIPESLTDFGAGAWLGLPRTLYVLGVVSLLVWYLLEHTPYGRELHAVGA
                     NALAARLVGIRVDRIVILSFVTTGALVGFAGVLQVARQGGGNPQIGASFTLPVLSAAF
                     LGATTVHPGRFNVPGTLLAVFFLATSVSGLALAGVDNWVESLFNGTALVLAVGLSTVL
                     GRRLSGI"
BASE COUNT          708 a         1552 c         1519 g          699 t
ORIGIN      
        1 cccggtcgcg gcgccatcgc aggcccgcgc cagtgcgacc ttgaccagat cgacgccgcc
       61 cagcgccagc agctcgccgc tcgcccgccg cccggggtcc agcccctgcg agcgaaatgt
      121 acctccaagg gccgcgcaac ccattgcggc cgttccggtt tgcgttttcg gtcagacagg
      181 ctcagtgtag agcctcgtcg ggtctggacg tcgccggccc ggtgcgaata cctcagtcgc
      241 cggaggacga gtatgctcaa ccatcgagat gcagcattcg gtcgaacgct ggtggccgcg
      301 acgctgattg cgtgcgcgtt catcggatgc aagaagaccg agagcgccgg agtgtccacg
      361 ggctcgggcg agccggcggt cgccgcggcc aaggcgaccg ccgagacgaa gcccgcggac
      421 aatgcggtcg atcccgcgat gcttgccgcc gccaagaccg agctcgatcg ggcctaccag
      481 ggcaccgagc gcgagccacc caagagcgcc gccaagccgg caccgggcaa gaaggtctgg
      541 atcatctcgc ccggccagat cggcgagagt gcgtcgatcg cgaccaacgc ggccaaggaa
      601 gccggcgagc tgatcggctg gaagatgacg ctgtacgaca gcaagctcga cgtcgcgaac
      661 ttctcgctgg ggatccgcca ggcggtcgcg gcgcaggccg acggcgtgat cctgcactcg
      721 atcgactgcc cgctggtcaa gcaagcgctg gccgaggcgc gcgccgcgaa ggtcaagatc
      781 gtcgcctact atgccctcga ttgcgatgat ccgtcggtca aggggcaacc gatgtacgac
      841 gcatcggtga acttcggcag tcagttcggc gactatgccg cgctgacccg cgcgtggggc
      901 gcggccaagg cggactgggt gatcacgaag accaagggtc gcgccaaggt gatctcgttc
      961 aaggaagacg agctcttggt cgtgaagtat atccgcgagg gcttcgagca agagctcgca
     1021 aagtgcaaga cctgcgagat cgtcaagacc gtggacttca cgctcgcgga cctcgggccc
     1081 aagctgcagc agaaggcgca gagcgcgctg ctccagcacc ccgaagccaa tgcgatgcac
     1141 gtgccgtacg actctgcgat gctactgggg atctcgggcg cactgctcga gtccaatcgc
     1201 aacgatcaga tcgaggtcat cgcgggggaa ggctttccga ccaacctcca gatgatccgc
     1261 gacaacaagg gccaggatgc ggccaatgcg ttcccggccc agtggaccgg ctacgcggcg
     1321 atcgatacgc tcaacagctt gttcgccgga cagcctgcgc aggattcggg gatcgggtac
     1381 cagatgatcg atcgcgagca caacctgcca ccggcaggga aaggctacga gcccaaggcc
     1441 gactacaagg cagcgtacaa gaaagcgtgg ggagtcgctc cgtagtgcag agcggcaccg
     1501 tcgttcgcgt cgagaacatc agcaagacgt tcccgggcac ccgcgcgctc gaccgcgtga
     1561 ccatcgagct tgcgcgcggc gagatccacg cactgctcgg caacaacggc tcggggaagt
     1621 cgacgctgat caagatcctg gcaggcgtcc atcgcgccga tccgggcggc gagatccatg
     1681 cgggcggccg gcgcttcgcg gccgaccatt tcacgcccga tctggcgcgc agcgcaggcc
     1741 ttcacttcgt gcatcagaac ccggcgctgt tcccgatgct cagcgtcgcc gagaacctcg
     1801 cgctgggccg cggctacgag acccgtcgcg gacgcatcga ctggcgccgg cagaacgcgc
     1861 gcgccgcgcg gctgatcgag cggtttcaca tccgcgccac gcccgagcag ccggcggtcc
     1921 tgctcgcgcc gccggatcgc acgctggtgg cgatcgcccg cgcgctgcag gatcaggacg
     1981 gcgagcacga gggcgtgctg atcctcgacg agccgacctc ggcgctggcg gcgccggagg
     2041 tcgagcgcct gctgtcgacc ctgcgcggct atgcccgtgc cgggcagacc atcgtgttcg
     2101 tgagccaccg gctcgacgag gtgctcggct gcagcgaccg ggtcagcgcg ctgcgcgacg
     2161 gccgtcatgt cggcaccgcg aacacgcggg acatgaccga gaccacgctg gtcgagatga
     2221 tgctagggcg ccacgtcgag ccaccgcgcg tccaccggca cggcgagccg cgatccgcgg
     2281 cgctgtcggt gaccggtctc gccggccctc aggtcagcgg cctcagcttc gagctcggcc
     2341 gcggcgaggt gctcggcctc gccggcctgc tcggtgccgg cgcgtcgaac gtgctgcgcc
     2401 agctgttcgg cgccgagcgc cgcgctgcag gcgagctgca gctgttcggc caaccctacg
     2461 caccggccgc cccgcaggac gcgatcgcgc gcggcgtcgc ctacgtgccg cccgatcgcg
     2521 cgatcgagag cagctttcac ggcatgagcg tgcgcgccaa cctgtccgcg gtgaacgtac
     2581 ggcggtactt ccagggcctg cggcttcgtc acggtcagga gcacgcggat gcgcgggccg
     2641 cgatcgaccg attccagatc cagctggcat cggatcgcca gggcttcgcc acgctgtcgg
     2701 gcggcaatca gcagaaggtg atcgtggcgc gctggctacg cgaccagccc aggttgctct
     2761 tgctcgacga accgacccag ggcgtcgcgg tgcacgcccg ccgcgaaatc cacggcttcc
     2821 tgcgcgacgc cgccgcgcgc ggcgtggcga tcctggtcgt gagctcggac ttcgaggagc
     2881 tcgcgcagct atgcgaccgc gtgctcgtga tcgtcggcgg ccgctgcgcg ggcgaggtca
     2941 cggcagaccg gctgagcgct caccagctca ccgagctcgc ccacttcatg ccggagcaat
     3001 caccatgagc gaggtactcg acccgaccgc cacgtccgcg agcgcacccg ccgagcatgc
     3061 gccgcgcaag cctgccggta cgcgcggtat cgtgttcgcc gtgcagcgct acggcctggc
     3121 gctgctgttc gcgctggtcg tcctcggctt cagcgtgatg atgccggagt cgttcgcgac
     3181 cgtgctcaac ttccgcaaca tcacggccaa tcagtcggtg ctcgcgatcg tcacgctcgc
     3241 agcgatcatc ccgctgatcg gtggcaactt cgatctatcg atcggcgcgg ggctgggcgt
     3301 cacctccatc gcgacggcgt cgctcctgtc gcacggcttg ccgctgtggc tcgcgatgct
     3361 cgtcggcatc gcgctcggcg ccacgatcgg gctgttcaac ggcctgctgg tcgccaaggt
     3421 cggcgtcaac gcgctgatca ccacgctcgg cgtcgccacg atcctcaccg ggctcacggt
     3481 gaagtacact ggcgggctga gcatcatcac cggcatcccc gagagcctga ccgatttcgg
     3541 cgccggcgcg tggctcggac tgccgcgcac gctctacgtg ctgggcgtcg tctcgctgct
     3601 cgtgtggtac ctgctcgagc acacgccgta cggccgcgag ctgcacgccg tgggtgcgaa
     3661 cgcgctcgcc gcgcgcctgg tcggcatccg ggtcgaccgc atcgtcatcc tgagctttgt
     3721 cacgacgggc gcactggtcg gctttgccgg cgtgctgcag gtcgcgcggc agggcggcgg
     3781 caacccgcag atcggcgcct cgttcacgct gcccgtgctg tcggcggcgt tcctcggcgc
     3841 gaccaccgtg caccccggtc gcttcaacgt gccgggcacg ctgctcgccg tgttcttcct
     3901 ggccaccagc gtgagcggtc tcgcgctcgc gggcgtcgac aactgggtcg agtcgctgtt
     3961 caacggcacc gccctggtcc tcgccgtcgg actgtcgact gtgctcggcc ggcgcctgag
     4021 cgggatctga gcggcgtcgc gccgtgcgac cagcatattc acgtcgacgc gttgtgtcgc
     4081 gatgcttaca ccgagttcca acgtatgccc gctgacacaa ccggcatccg gtggtgctcg
     4141 ccggctcgcc ggttccacct cggggtactt gattgatacc tccagctggg cgacggtccc
     4201 aagcgacaaa cggccgtcgc ccgagcggag cggcttcgag gagacgaata tgagatccgt
     4261 gagattgctg ccgtgcgcgg ccatgttggt ggttacttcg gtcggctgtg gagacgtccc
     4321 gagcctggag gaccgcccct cgctcacaac cgcgcagtcc tcattgctga tcgggggcgg
     4381 gtgtccggca tctggcttgg acggcgaccg ggatcagatc gatgacggcg tggagaattg
     4441 cctgctccaa cgcttcgcgc cggtcgtaca ccttgcag
//
LOCUS       DASVOR010001990         6839 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_22133,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001990 DASVOR010000000
VERSION     DASVOR010001990.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6839
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22133"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..547)
                     /locus_tag="VF516_36930"
     CDS             complement(173..547)
                     /locus_tag="VF516_36930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113391.1"
                     /translation="MTSLARIALALVVAPVLVFTAACDEEADKPETAQRAAMCRQLMA
                     HIFQITPRPGSDRPETDPARIQELVARVPIEDIEQCAAVKDAVKPGEPPPPETQTPKV
                     IACMQAATDARALRRCIPAQAE"
     gene            complement(544..2190)
                     /locus_tag="VF516_36935"
     CDS             complement(544..2190)
                     /locus_tag="VF516_36935"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEX8113392.1"
                     /translation="MPRDQDTGFFGHPSGLSTLFFTEMWERLSFYAARAFLAIYMTKE
                     VALGGRGMSDAAAGAVLALYLSSGYLLSLLGGWIADRFLGQRKAVTVGGVGIVIGNAL
                     LAAPSDDLFYPGLVIVAVGTAFLKPNISTIVGQLYRPEDNRRDSGFTIYYMGINIGAG
                     LAPLVGTLMAQSPGFRHLLANHGIDPNMCWKFAFAVPAIGMTVGLIQYALGYRKLGDA
                     GLRPTVPSDPAKAARDRKVLVGVGGGLVGLVVAAFAYDRLVHPLTKGLLVNVFGVALL
                     VTAVAIFLGFFRTARDAGERQRVIAMIPLFIGCVAFFALFEQAASTLSLFAERLVHRD
                     YLGLHVPASAYQFINSAFVVLLASAFAWMWLRLARNGREPSTVTKFAIAMVLNAVSYV
                     VLLPSLSTVSVVDGVNPDYLFPFPFRTVSPNFLIGYYFVATCAELCLSPVGLSSFSRL
                     APARLAGMVMGTWFLGISIGEYLAGRAAEVSGSRGYGFLFTVVILGSLVVAVALFAVA
                     PAIKRMLSRDAQQPAELPRAVVEPKAKESAAQGSAEGGRP"
     gene            complement(2311..2625)
                     /locus_tag="VF516_36940"
     CDS             complement(2311..2625)
                     /locus_tag="VF516_36940"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8113393.1"
                     /translation="MALRVRVCTLDQVAGDGLHAFAVPGVTWPVIVTRVDGALVAVPG
                     VCPHEDVALAGGELAGGAIVCPGHGYAFDLRTGRCKHDPALELRRYPITVIGRELWVD
                     LL"
     gene            complement(2625..3512)
                     /locus_tag="VF516_36945"
     CDS             complement(2625..3512)
                     /locus_tag="VF516_36945"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8113394.1"
                     /translation="MDPAQAARPRRPAGRTLSPSAWLGLAMLAVFAAAGIFGPIIAPH
                     DLSLPHVELAHQFEAGSAAHWLGTDSSGRDALSQLLWGARSALELSVIVVAISSVLGL
                     AIGTAAGWFGGAVDEVVMRVVDVLMAFPGILLNIAIVATVAHPGVPLTIAALCANGWV
                     GYARVARGQVLALRDRDYVTAAVALGASHRRVMARHLVPNLMAPALVQMSFGLGSVIL
                     IEASLSFLGIGPQLDYTWGAMLEQARNFLWNTGWVRVYALVPGLAITWVVLGANLLGD
                     GLRDRLDPRQRGRDGGAGR"
     gene            complement(3521..4459)
                     /locus_tag="VF516_36950"
     CDS             complement(3521..4459)
                     /locus_tag="VF516_36950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8113395.1"
                     /translation="MTIGPFLVRRLGSGLLAILGASLLTFSFLHLVPGDPVDHLAGGD
                     APPGMRAAIERCMELDRSLPAQFVGFLGHVVDGTLGHQCKNGDPGGPTVAARIAEVMP
                     HTLALAVCGMLVAVALALPLGVAAAIRRGSWLDTAATVVSLSGIAVPQMMFAPLLLLW
                     GFVSLGWFPGPTEIGPRAIVLPALAVGTHLMAMLARMTRSSMVEVLGEDYVRTARAKG
                     LPEGRVLVLHALRNALLPVITIAGLQFGSLLSGAIIIENVFSRPGLGSTLVEAIRERN
                     YPVVQGAVLVIAAIYVAVNLAVDLGYGLADPRIRRA"
     gene            complement(4488..5012)
                     /locus_tag="VF516_36955"
     CDS             complement(4488..5012)
                     /locus_tag="VF516_36955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113396.1"
                     /translation="MGSRHVLLALAGLLAAGALVGGCGKEIGDACTLASDCSPNGDRI
                     CQCSNCSGTDPTGDSAKGYCTIQGCDFGTCPGEAVCVRFFTGSFDNKPCTPQTADTDC
                     SIDELCSLAGNCVPRSAEVRYCMLKCDSGGDCRDNFECRDKTLMMEHGGEPVPQQGTT
                     LNADNPPKFCAAKP"
     gene            complement(5160..6134)
                     /locus_tag="VF516_36960"
     CDS             complement(5160..6134)
                     /locus_tag="VF516_36960"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8113397.1"
                     /translation="MHVVVTGAAGFIGSHTCERLVAAGHRVTGIDAFDGYLYPAEVKR
                     ATAAELAGLPGFRLVEADICDRAAVAGVIDGGVDVVCHLAALAGVRPSLAEPLRYLRT
                     NIEGTGVIIERMRAVGRKRLVFASSSSVYGARHGTDLREIAAFREDDPCLSPASPYAA
                     TKRMNELQLSTYRDLFGLGVHALRFFTVYGPRQRPDMAIAKFTRAIARGEPIALFGDG
                     TTRRDYTFIDDIVRGVVASIGAVSPGCYELINLGGTHTTSLRELVEILERVIGRPAVI
                     DWQPSQPGDVPVTYASIDRARALLGYAPTTRPEVGIARYWAWYQGRSA"
     gene            6243..6695
                     /gene="dtd"
                     /locus_tag="VF516_36965"
     CDS             6243..6695
                     /gene="dtd"
                     /locus_tag="VF516_36965"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEX8113398.1"
                     /translation="MRAVVQRVTRARVTVDDRITGEIGHGLLVLLGAGAGDTAADLGY
                     IVDKVAHLRIFADDAGKMNRSVLDVAGGVLVVSQFTLYGDARQGRRPAFTGALEPAAA
                     RALCEQAVVRLREAGVTRVEAGEFAAHMAVELVNDGPVTILLDSRKGF"
BASE COUNT         1043 a         2532 c         2449 g          815 t
ORIGIN      
        1 cgccgagccg tcgcggatcc cgccgctcgg cctccacccg agccacccca acggcatggt
       61 gctggtcgcc gagtcgacct gggactggat cgtcgaccgg ctgcccgccg ccgaccgcgc
      121 gcgctgccgg gcctggccga cgctgtgagc ccagggcttg gcgccggcgc tgctactcgg
      181 cctgcgccgg gatgcacctg cgcagcgctc gggcgtcggt cgccgcctgc atgcacgcga
      241 tcaccttcgg cgtctgggtc tccggcggcg gcggctcgcc cggcttcacc gcgtccttga
      301 ccgccgcgca ctgctcgatg tcctcgatcg ggaccctggc gacgagctcc tggatccgcg
      361 ccggatcggt ctccggccgg tcgctgcccg gccgcggcgt gatctggaag atgtgcgcca
      421 tcagctgccg gcacatcgcc gcgcgctgcg cggtctccgg cttgtccgcc tcctcgtcgc
      481 aggccgccgt gaacacgagg accggggcga ccaccagggc gagcgcgatg cgggccaggg
      541 acgtcatggg cggcctcctt cggcggaccc ctgggcggcg gactccttgg ccttgggctc
      601 gacgaccgct cggggcagct cggccggctg ctgcgcgtcg cggctcagca tgcgcttgat
      661 cgccggcgcg acggcgaaca gcgccaccgc gaccaccagc gagccgagga tcaccacggt
      721 gaacaggaag ccgtagccgc gcgagccgct gacctcggcg gcccggcccg cgaggtactc
      781 gccgatcgag atcccgagga accacgtgcc catcaccatg ccggcgagcc gcgccggggc
      841 cagccgcgag aagctcgaca gcccgaccgg cgacaggcac agctcggcgc aggtcgcgac
      901 gaagtagtag ccgatcagga agttcgggct gacggtgcgg aacgggaacg ggaacaggta
      961 gtccgggttg acgccgtcga cgaccgacac cgtcgacagg ctcggcagca gcacgacgta
     1021 cgacaccgcg ttgagcacca tggcgatcgc gaacttcgtg acggtcgacg gctccctgcc
     1081 gttccgggcc agccgcagcc acatccacgc gaacgccgag gcgagcagca cgacgaacgc
     1141 cgagttgatg aactggtacg cgctggccgg cacgtggagc ccgaggtagt cgcggtgcac
     1201 caggcgctcg gcgaacagcg acagggtcga cgcggcctgc tcgaacaggg cgaagaacgc
     1261 gacgcagccg atgaacaagg ggatcatcgc gatcacgcgc tggcgctcgc cggcgtcgcg
     1321 cgcggtccgg aaaaagccga ggaagatcgc caccgcggtg accagcagcg cgaccccgaa
     1381 cacgttgacc aggaggccct tggtcagcgg gtgcacgagg cggtcgtacg cgaacgcggc
     1441 gacgaccagg ccgaccagcc cgccgccgac cccgaccagc accttgcgat cgcgcgcggc
     1501 cttggccgga tcgctcggca cggtcggccg cagcccggcg tcgccgagct tgcggtagcc
     1561 cagcgcgtac tggatcaggc cgacggtcat gccgatcgcc ggcaccgcga acgcgaactt
     1621 ccagcacatg ttggggtcga tgccgtggtt ggcgagcagg tggcggaacc ccgggctctg
     1681 cgccatcagc gtcccgacca gcggcgcgag gccggcgccg atgttgatcc ccatgtagta
     1741 gatcgtgaac ccgctgtcgc ggcggttgtc ctcgggacgg tacagctggc cgacgatcgt
     1801 cgagatgttg ggcttgagga acgcggtgcc caccgcgacg atcaccaggc cgggatagaa
     1861 caggtcgtcg gacggcgccg cgagcagcgc gttgccgatc acgatgccga cgccgcccac
     1921 cgtcaccgcc ttgcgctggc cgaggaagcg gtccgcgatc cagccgccga gcagcgacag
     1981 caggtagccc gacgacaggt acagcgcgag caccgcgccg gccgccgcgt cggacatccc
     2041 gcgcccgccg agcgcgacct ccttcgtcat gtagatcgcg aggaacgcgc gcgcggcgta
     2101 gaacgacagc cgctcccaca tctcggtgaa gaacagcgtc gacaggccgg acgggtgccc
     2161 gaagaagccg gtgtcctgat cccgtggcat cgcagcgaca ctagcgcgaa ctcgccgcgc
     2221 ggtgcggggc aaagcggcag gccgcggccg gtcgcagcga cgcgcgcgct cgccgacgcc
     2281 cctgctgggc ggcagaggtg tggcgaggcg ctacagcagg tcgacccaca gctcgcggcc
     2341 gatcaccgtg attggatagc gccggagctc cagggccggg tcgtgcttgc agcgcccggt
     2401 gcgcaggtcg aacgcatagc cgtggccggg gcagacgatc gccccgcccg ccagctcgcc
     2461 gccggcgagc gcgacgtcct cgtgcgggca caccccgggg accgcgacca gcgcgccgtc
     2521 gacccgggtg acgatcaccg gccaggtcac gcccggcacg gcgaacgcat gcagcccgtc
     2581 gccggcgacc tgatcgagcg tgcagacacg gacgcgcagc gccatcagcg acccgcgccc
     2641 ccgtcgcggc cgcgctgccg cggatcgagc cgatcgcgca ggccgtcgcc gagcaggttg
     2701 gcgccgagca cgacccaggt gatcgcgagc ccggggacca gcgcgtagac ccggacccac
     2761 ccggtgttcc acaggaagtt gcgcgcctgc tcgagcatcg ccccccaggt gtagtcgagc
     2821 tgcggcccga tgccgaggaa cgacagcgac gcctcgatca ggatcacgct gccgagcccg
     2881 aacgacatct ggaccagcgc cggcgccatc aggttgggca ccaggtgacg cgccatgacc
     2941 cggcggtgcg aggcgccgag cgcgaccgcc gcggtcacgt agtcgcgatc gcgcagcgcg
     3001 agcacctggc cgcgcgcgac ccgggcgtag ccgacccagc cgttggcgca cagcgccgcg
     3061 atcgtgagcg ggaccccggg gtgcgcgacg gtcgcgacga tcgcgatgtt gagcaggatg
     3121 ccggggaacg ccatcagcac gtcgaccacg cgcatcacga cctcgtcgac cgcgccgccg
     3181 aaccagccgg ccgcggtgcc gatcgccagg ccgagcaccg acgagatcgc gacgacgatc
     3241 accgacagct ccagcgccga ccgcgcgccc cacaagagct ggctcagcgc gtcgcggccg
     3301 ctgctgtcgg tgccgagcca gtgcgccgcc gagccggcct cgaactggtg cgcgagctcg
     3361 acgtgcggca gcgacaggtc gtggggcgcg atgatcggcc cgaagatccc ggccgccgcg
     3421 aacaccgcga gcatcgccag gccgagccac gcgctgggcg acagcgtccg gccggccggt
     3481 cgccgcggcc gggccgcctg cgctgggtcc acggccgcca ctacgccctc cggatccgcg
     3541 ggtcggcgag cccgtagccc aggtcgaccg ccaggttgac cgcgacgtag atcgccgcga
     3601 tcaccagcac ggcgccctgc accacggggt agttgcgctc gcggatcgcc tcgaccaggg
     3661 tcgagcccag cccggggcgc gagaacacgt tctcgatgat gatcgcgccc gacaggagcg
     3721 agccgaactg caggccggcg atcgtgatca ccggcaggag cgcgttgcgc agcgcgtgga
     3781 gcaccagcac gcggccctcg ggcaggccct tggcgcgcgc ggtgcgcacg tagtcctcgc
     3841 cgagcacctc gaccatcgac gaccgcgtca tccgcgccag catcgccatc aggtgggtgc
     3901 cgaccgcgag cgcggggagc acgatcgcgc gcggcccgat ctcggtcggg ccggggaacc
     3961 agcccagcga gacgaacccc cacaggagga gcagcggcgc gaacatcatc tgcggcacgg
     4021 cgatccccga cagcgacacc acggtcgccg cggtgtcgag ccacgacccg cgccggatcg
     4081 cggcggcgac cccgagcggc agcgcgagcg cgaccgcgac cagcatgccg cacaccgcga
     4141 gcgccagggt gtgcggcatc acctcggcga tccgcgccgc cacggtcggc ccgccggggt
     4201 cgccgttctt gcactggtgg ccgagcgtac cgtcgaccac gtggccgagg aacccgacga
     4261 actgcgccgg cagcgagcgg tcgagctcca tgcagcgctc gatcgccgcc cgcatccccg
     4321 gcggcgcgtc gccgcccgcg aggtgatcga ccggatcgcc cggcaccagg tgcaggaacg
     4381 agaacgtcag cagcgaggcg cccaggatcg ccaggagccc cgagcccagc cgacgcacca
     4441 ggaacgggcc gatcgtcacg ccgcaatcgt atcgcgcggc cggagcacta cggcttcgcg
     4501 gcgcagaact tcggcgggtt gtcggcgttc agcgtggtcc cctgctgagg caccggctcg
     4561 ccgccgtgct ccatcatcag cgtcttgtcg cggcactcga aattgtcgcg gcagtccccg
     4621 ccggagtcgc acttgagcat gcagtagcga acctccgccg agcgcggcac gcagttgccg
     4681 gcgagcgagc acagctcgtc gatcgagcag tcggtgtccg cggtctgggg ggtgcacggc
     4741 ttgttgtcga acgagccggt gaagaaccgg acgcacaccg cctcgcccgg gcaggtcccg
     4801 aagtcgcagc cctggatcgt gcagtagccc ttcgccgagt ctccggtcgg atcggtcccg
     4861 ctgcagttcg agcactggca gatccggtcg ccgttggggc tgcagtccga ggccagcgtg
     4921 caggcatcgc cgatctcctt gccgcagccg ccgaccagtg cgcccgcggc cagaaggccg
     4981 gcgagcgcga gcagaacatg acgggacccc atgggcgcgc gcatcctatc ggcggttggg
     5041 acccgcggca accgcacgag gttgcctcgc acgcgccgcg gcgtttcccg tgcaattgcg
     5101 gccacaacgc cccggcgtgc ggcgtggcac ggcgcccggg cgcggcgccg gagccggcgt
     5161 cacgcagagc ggccctggta ccacgcccag taccgcgcga tgcccacctc ggggcgggtc
     5221 gtcggcgcgt agccgagcag cgcccgggcg cgatcgatgc tggcgtaggt caccggcacg
     5281 tcgcccggct ggctcggctg ccagtcgatc accgccggcc ggccgatcac ccgctcgagg
     5341 atctcgacca gctcgcgcag cgaggtcgtg tgggtgccgc cgaggttgat cagctcgtag
     5401 cagcccggcg acaccgcgcc gatcgacgcg accaccccgc ggacgatgtc gtcgatgaac
     5461 gtgtagtcgc gccgggtcgt gccgtcgccg aacagcgcga tcggctcgcc gcgcgcgatc
     5521 gcgcgggtga acttggcgat cgccatgtcg gggcgctggc gcggaccgta caccgtgaag
     5581 aaccgcagcg cgtggacccc gagcccgaac aggtcgcggt aggtcgacag ctgcagctcg
     5641 ttcatccgct tggtcgcggc gtacggcgac gccggtgaca ggcacggatc gtcctcgcga
     5701 aacgcggcga tctcgcgcag gtcggtgccg tgccgggcgc cgtacaccga gctcgacgac
     5761 gcgaacacca ggcgctttcg cccgaccgcg cgcatccgct cgatgatcac gccggtgccc
     5821 tcgatgttgg tgcgcaggta gcgcagcggc tcggcgagcg acggccggac cccggcgagc
     5881 gccgcgaggt ggcacacgac atccaccccg ccgtcgatca cgccggcgac cgcggcgcga
     5941 tcgcagatgt cggcctcgac cagccggaac ccgggcagcc cggcgagctc ggcggcggtc
     6001 gcgcgcttga cctcggccgg atacaggtag ccgtcgaacg cgtcgatccc ggtgacccgg
     6061 tggccggccg cgaccagccg ctcgcaggtg tgcgatccga tgaacccggc ggccccggtg
     6121 acgacgacgt gcatggtgcg actgtcgcgt ggccgcccgc ggcccgcaag tcacgacggc
     6181 gctgtgcgct ctgcgccgcg gcccgcgttg catccggcgc cccgccgcgc gacaaccgcc
     6241 gcatgcgcgc cgtcgtccag cgagtcaccc gcgcccgggt cacggtcgac gaccggatca
     6301 ccggcgagat cggccacggc ctgctcgtct tgctcggcgc cggcgccggc gacaccgcgg
     6361 ccgacctcgg ttacatcgtc gacaaggtcg cccacctgcg gatcttcgcc gacgacgccg
     6421 gcaagatgaa ccgcagcgtg ctcgacgtcg ccggcggcgt gctcgtcgtg tcgcagttca
     6481 cgctctacgg cgacgctcgc cagggccgcc gcccggcgtt caccggcgcg ctcgagcccg
     6541 cggccgctcg cgcgctgtgc gagcaggcgg tggtccggct gcgcgaggcc ggcgtcaccc
     6601 gggtcgaggc cggcgagttc gcggcccaca tggcggtcga gctggtcaac gacggcccgg
     6661 tgacgatcct gctcgattcg cgcaagggat tctagcccca ggcccccggc ggggttacgg
     6721 tccggatggc gcccggcgcc cacgatgaag gtcaggttct ggggagtccg tggttcgatc
     6781 cccgtacccg ggcgcacgac cagccggtac ggaggcaaca cgtcgtgtgt cgaggtccg
//
LOCUS       DASVOR010001991         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_196503,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001991 DASVOR010000000
VERSION     DASVOR010001991.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196503"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /locus_tag="VF516_36970"
     CDS             complement(<1..343)
                     /locus_tag="VF516_36970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113399.1"
                     /translation="MDPAVLPILLCPRCRTEAPFKDLRAPRCQACGLEPATSVPGVLD
                     LLDTGAGEPLSVTTEQRLMESELVARTYDRFWRPTFVRLLAGKGAGAAVGGLSGELFI
                     HKHGLGLDDRAG"
     gene            535..933
                     /locus_tag="VF516_36975"
     CDS             535..933
                     /locus_tag="VF516_36975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HEX8113400.1"
                     /translation="MRRFRIVICRGPECGDRHGSAALHDAFQAQLAAHGAQAHTELTW
                     QSCFGRCTQGPNVLVREILTCEAAPTLGTGFATLPGPRGTTALYNRMDGQRVACVVGA
                     HVMGGQIVREYIERPGIHPSDPVAVRTKDT"
     gene            936..1517
                     /locus_tag="VF516_36980"
     CDS             936..1517
                     /locus_tag="VF516_36980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113401.1"
                     /translation="MTRTSIVTTLTVLVTLGTAAGCKGEKVIQPDPKTQADLDQCLKD
                     KAEKDKLIKAEEDENAALMREKSSGKEIVVAIEGNLMTVKPGAPGEVRPIDDKVVGEA
                     AKQFQNVVEKSRGAIQKCYEQALKKDTGLQARTVTLSVSASFTSGQFKGSNFAPSLGD
                     AFDACIRTVASKWTLPPSAPAMTFKAQVSLTPS"
     gene            1550..>1829
                     /locus_tag="VF516_36985"
     CDS             1550..>1829
                     /locus_tag="VF516_36985"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113402.1"
                     /translation="MTKLKELEQRLRSEPDNLGLRVAVAGALHEAGRRADAVELYRSV
                     AVAYRDQGRPQQAITVCRSILELAPEDAPCQELLAALLANQAAPRASSP"
BASE COUNT          307 a          635 c          657 g          230 t
ORIGIN      
        1 gcccggcccg gtcgtcgagc cccaggccgt gcttgtggat gaacagctcg ccggacagcc
       61 cgccgaccgc cgcgccggcg cccttgccgg cgagcagccg cacgaaggtc ggccgccaga
      121 accggtcgta ggtccgcgcc accagctcgc tctccatcag gcgctgctcg gtggtcaccg
      181 acagcggctc gccggcgccg gtgtcgagca gatcgagcac gccgggcacc gaggtcgccg
      241 gctccagccc gcaggcctgg cagcgcggcg cccggaggtc cttgaacggg gcctcggtgc
      301 ggcagcgcgg gcacagcaag atcggcaaca cggcaggatc cacgcggtgg ttgtagctga
      361 aaccccgcgt cgggactcga cccccacccg cgatctgccg ggcccccgcc gcgcgccggc
      421 gcaacccgat cgcgcgcgaa tcttgaccct gctcaccgct ggtcggccgg ggacggtgtc
      481 gcaccaagag ccgcgattga caggccgccg ggcgccgttc accctcaagg gcacgtgcgc
      541 cggttccgca tcgtgatctg ccgaggcccc gagtgcgggg atcgtcacgg atcggccgcg
      601 ctgcacgacg cgtttcaggc ccagctggcg gcgcacggtg cgcaggcgca caccgagctg
      661 acgtggcaga gctgcttcgg ccgctgcacc cagggcccca acgtgctggt gcgagagatc
      721 ctcacgtgcg aggcggcgcc cacactgggc accgggtttg ccacactacc gggcccgcgg
      781 ggcacgacgg ccctgtataa tcgaatggat ggccagcgcg tcgcgtgcgt cgtcggcgcg
      841 catgtgatgg gagggcagat cgtccgcgag tacatcgaac gacctggcat ccatccgagt
      901 gaccctgtag ccgtgaggac gaaagacacc tagccatgac ccggacatcg atcgtcacaa
      961 cgctgaccgt cctggtgacc ctggggaccg ctgcggggtg caagggcgag aaggtcattc
     1021 aacccgatcc gaagacccag gccgatctgg accagtgcct caaggacaag gcggagaagg
     1081 acaagctgat caaggccgag gaggacgaga acgccgcgct gatgcgcgag aagagcagcg
     1141 gcaaggagat cgtggtcgcg atcgagggca acctgatgac cgtcaagccg ggcgcgcccg
     1201 gtgaggtccg gccgatcgac gacaaggtgg tcggcgaggc ggccaagcag ttccagaacg
     1261 tggtcgagaa gtcgcgcggc gcgatccaga agtgctacga gcaggcgctc aagaaggaca
     1321 ccggcctgca ggcgcggacc gtgaccctgt cggtgtcggc gagcttcacg tcgggccagt
     1381 tcaagggctc gaacttcgcg ccgtcgctcg gcgacgcgtt cgacgcctgc atccggaccg
     1441 tcgcgagcaa gtggacgctg ccgccgagcg cgccggcgat gacgttcaag gcccaggtct
     1501 cgctgactcc atcgtagccg gagccgccgg gatcgacgat ggtcgcgcca tgaccaagct
     1561 caaggagctg gagcagcgcc tccgcagcga gcccgataac ctcgggctgc gggtcgcggt
     1621 ggcgggcgcg ctgcacgagg ccgggcgccg ggccgacgcc gtcgagctct accgctcggt
     1681 ggcggtggcg taccgcgatc agggacgccc gcagcaggcg atcacggtgt gccgcagcat
     1741 cctcgagctc gcgcccgagg atgcgccgtg ccaggagctg ctcgccgcgc tgctcgcgaa
     1801 ccaggcggcg ccgcgcgcgt cgtcgccac
//
LOCUS       DASVOR010001992         4231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_517391,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001992 DASVOR010000000
VERSION     DASVOR010001992.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517391"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..614
                     /locus_tag="VF516_36990"
     CDS             48..614
                     /locus_tag="VF516_36990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113403.1"
                     /translation="MAKHDTELADAAAGFDQELATYTRLGELFVHAPVGRVKQLERAN
                     TALAEIAACEERLQAAGQRMVEALAAARARQEQLARDVVARVPVVQDRNRRLQALMAD
                     LAGVATQVGALNTTLAGQRDNGDPTQPPTADAARDISVTLLGLSERASELATAARDAE
                     FEELATQAHALYQRLQAIGKKLHKAVGE"
     gene            complement(619..771)
                     /locus_tag="VF516_36995"
     CDS             complement(619..771)
                     /locus_tag="VF516_36995"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HEX8113404.1"
                     /translation="MLLALAAIIAIAWLLGFTVFHVASGAIHVLIVIAVIVAIVHFVQ
                     GRRAPL"
     gene            complement(857..1231)
                     /locus_tag="VF516_37000"
     CDS             complement(857..1231)
                     /locus_tag="VF516_37000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113405.1"
                     /translation="MPGEQAVLREPCGSCASPEAMLDLAALSEGNLSAPGQRRLAVHL
                     ARCRTCKWVLASLAGEPRAIAARRERGRAEVAAALDDALNDALNDALNDALNEALDDA
                     PDPSRWSPLSGRRVAPLSARGQ"
     gene            1582..2181
                     /locus_tag="VF516_37005"
     CDS             1582..2181
                     /locus_tag="VF516_37005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113406.1"
                     /translation="MRPTNRSRPLRPNAQWPPIVRDILEGDPSARTKARTILWQEVQC
                     FVERVVRLPIGPLNEDEEARRDIAVRVLVKLEKQSNAHLAAWYARQLRQRDHSSFWTW
                     IKTISWSCAIDHARTSRLNIAPRSEKRFDWIRIDCVDPQLLCEALGNPRSFLDCGADQ
                     DVESVLADLQAALRDDLAAAELVAYEDLSAIAGESSRSP"
     gene            2476..2844
                     /locus_tag="VF516_37010"
     CDS             2476..2844
                     /locus_tag="VF516_37010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113407.1"
                     /translation="MPRSQPHPALRHDRDYLELVTDIVIGPAAARGAAWAVLRRHVTD
                     YIEGFARLPVGWLNESELARRTIARRVVDRLAADSFDLLAQWRRRVLQGEDHAAFATM
                     LHIVIRWVAIRYAHATGCDP"
     gene            complement(2928..3194)
                     /locus_tag="VF516_37015"
     CDS             complement(2928..3194)
                     /locus_tag="VF516_37015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113408.1"
                     /translation="MTFDECSGFAIHDLAALAEGNLSPQGLQRLAGHLRRCQTCAATL
                     IAIVEDAHAGPGGEPHRLADWLSSVPWGNAARPGRADTGSAGKS"
     gene            3505..>4231
                     /locus_tag="VF516_37020"
     CDS             3505..>4231
                     /locus_tag="VF516_37020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113409.1"
                     /translation="MDSIELQICSRPPREPDRTCRPDQPGMRVTAIQGPIATPALRAS
                     AREPTLAQQLDALLTDVEFHKLIEAVTAEVVRRWNVPRRTAAGLVMSAVGEPGTLHCV
                     HRAWFLAETRGSGARLAKLIVRRRVFDLLRADARRPLHTSLPRTSDEIDGALRALDGR
                     IDRDSHAQAELHAVASRVRAALECFARQGPCQARQACLVRRHALDEIDRLDLAAQLAC
                     SQNALRVRIHKALRALRKHIEACH"
BASE COUNT          635 a         1510 c         1493 g          593 t
ORIGIN      
        1 cgccgtgcgt gctcgccgcg gccgatcgcg tgtagattgc aggccacatg gccaagcacg
       61 acaccgagct cgccgatgcg gcggccggct tcgatcagga gctcgccacc tatacccggc
      121 tgggcgagct gttcgtccac gcgccggtgg gacgcgtcaa gcaactcgag cgcgcgaaca
      181 ccgcgctcgc cgagatcgcc gcgtgcgagg aacgcctgca ggccgcggga cagcgcatgg
      241 tcgaggccct cgcggccgcg cgggcgcgcc aggagcagct cgccagggac gtggtcgccc
      301 gggtcccggt ggtgcaggac cgcaaccgcc ggctccaggc gctgatggcc gacctcgcag
      361 gggtcgccac gcaggtcggc gcgctcaaca ccacgctcgc cggccagcgc gacaacggcg
      421 acccgaccca gccgccgacc gccgacgccg cgcgcgacat ctccgtcacc ctgctcgggc
      481 tgtccgagcg cgcgtccgag ctcgccaccg ccgcgcgcga cgccgagttc gaggagctcg
      541 cgacccaggc ccacgcgctg tatcagcgcc tgcaggcgat cggcaagaag ctgcacaagg
      601 ccgtgggcga gtagcgggtc acagcggcgc gcggcggccc tgcacgaagt gcacgatcgc
      661 gacgatcacc gcgatcacga tcagaacatg gatcgcgccg gacgcgacgt ggaacacggt
      721 gaagccgagc agccaggcga tggcgatgat ggcagcaagt gcgagcagca tggggtcctc
      781 ctgcgcgaga gcactgcacg cctcaggcca ccggcatgcg aagctcgccg ccgctcgaat
      841 ctcgtagccg cgcgcgtcat tggccgcgcg cgctgagcgg ggcaacacgg cggccggaaa
      901 gcgggctcca gcgcgatggg tccggcgcat cgtcgagggc ctcgttgagg gcatcgttga
      961 gggcatcgtt gagggcatcg ttgagggcat cgtcgagggc cgcggcgacc tcggcccggc
     1021 cgcgctcgcg acgcgcggcg atggcgcgcg gctcaccggc cagcgatgcc aggacccact
     1081 tgcacgtgcg gcaccgcgcg agatgaaccg cgagccggcg ctgacccggc gcggacaggt
     1141 tgccctcgga gagcgcggcg agatccagca tcgcctcggg gctcgcgcac gatccgcagg
     1201 gctcgcgcag gacggcctgc tcgcccggca cgacggagta tcgaggtacg tgtggtacgt
     1261 ggggtacgtg tgtgcgaacg gacgaggaca ttgaatgtga ctcccaggtc ggcatgattc
     1321 ctccttcaac tggcccgcca gcgcttctcc aggacagcgc cggacgatcg ccttcgctga
     1381 agacggacga cagccgcgtg gacaaaaacg ccggtccggt gaaacgcacg cgatccccgc
     1441 tacaacgcaa acccggcggg ctgcgacagc gctgcgtgct ccggggcctg gcacacagca
     1501 gttccgcgac ttacccgcgc ggtcgcgttc ttgtcgtcct ccttgtggag tcggacccac
     1561 ccaaccacag gaggacaggc gatgcgccca acaaaccggt ctcggccact gcgccccaat
     1621 gcgcagtggc cgcccattgt ccgcgacatt ctcgagggcg atcccagcgc gcgaaccaag
     1681 gcgcgcacca tcctgtggca ggaggtgcag tgcttcgtcg agcgcgtggt ccggttgccg
     1741 atcggaccgc tcaacgagga cgaggaagcg cgccgcgaca tcgcggtgcg cgtgctggtc
     1801 aagctggaga agcagtcgaa cgcccacctc gccgcctggt acgcgcgcca gctgcgccag
     1861 cgcgatcact cctcgttctg gacctggatc aagacgatct cgtggtcgtg cgcgatcgat
     1921 catgcgcgga ccagccggct caacatcgcg ccgcgcagcg agaagcggtt cgactggatc
     1981 cggatcgact gcgtggatcc gcagctgctg tgcgaggcgc tcggcaaccc gcgcagcttc
     2041 ctcgactgcg gcgccgatca ggacgtcgag tccgtcctgg ccgacctcca ggccgcgctg
     2101 cgcgacgatc tggccgcggc ggagctcgtg gcgtacgagg acctgtccgc gatcgccggc
     2161 gagtccagcc ggtccccgtg accgcagcgc cgtgatgcag gcgagcgccg cggtcgtcgc
     2221 ggcggatccg gtcaggtccg gacgcggcac gcggcacgcg gcacgcggca ggctgctgtc
     2281 gtgccaggcc tgtctgcgcc ggcgtggcga tgccgctccg atcggcgctc ctcgcggtgt
     2341 cacggccgag cggggctgtg acgggttgag cacgagccgg gaagccgtga gacgcatcgg
     2401 cgcacggcgt gcgacgcccg gaggttttct gtcgtcctga actgatgacc cggaggtttc
     2461 gaccggaggt ttctcttgcc gcgatcacaa ccgcaccccg cgctccgaca cgaccgcgac
     2521 tacctcgagc tcgtcaccga catcgtgatc gggccggccg ccgcgcgcgg cgccgcatgg
     2581 gcggtgctgc gccgccacgt gaccgattac atcgagggtt ttgccaggtt accggtcggc
     2641 tggctcaacg agtccgagct ggcgcgccgg accatcgccc gccgcgtcgt cgaccggctg
     2701 gcggccgact cgttcgacct cctggcgcag tggcgccggc gcgtgctgca aggcgaggat
     2761 cacgccgcat tcgccacgat gctccacatc gtgatccggt gggtcgcgat ccggtacgcc
     2821 cacgccaccg gctgcgatcc ctgacgtcgg ccgcgctgtc tcacaggacc gaagaagatc
     2881 ctcatgcaga gagcgttcac gaacaggaag atccggtcgt cggccggtca cgacttgccg
     2941 gcgctgccgg tgtccgcgcg tcccggccgg gcggcgttcc cccacgggac cgaggacagc
     3001 cagtccgcca ggcggtgagg ttcacccccc gggccggcgt gtgcatcctc gacgatcgcg
     3061 atcagggtcg ccgcgcaggt ctggcaccgg cgcaggtggc cggcgagccg ctgcagcccc
     3121 tgcggcgaga ggttgccctc tgccagcgcg gccagatcgt gaatggcaaa gcccgagcat
     3181 tcatcgaatg tcacgggcgc cggatccggc ctggaggcct gaagctgcaa ggggtgtaac
     3241 cgattgcctc gtggtcgggc catcttcaat gctccttccg tgccaccgcg cccgcggtcg
     3301 cttcccggat ggcgcgctgc gatcgagcgc gtcgtttccc ctctatcgcg cggtgcccgg
     3361 cgagcattag ctcgtggcgc cgaaatggtc tgcgattttc cgacgggagc catcgatcgc
     3421 cggtgacgcg gccgcgcacc cgccccacgg cggctccgtg cgccccggcg cgatctcccg
     3481 gtccgaccac accgcccatt gccgttggat tccatcgagt tgcaaatttg ttcacgaccg
     3541 cctcgcgagc cggaccgaac ctgtcgtccg gatcagccag gcatgcgtgt cacagcgatc
     3601 cagggcccga tcgcgacgcc ggcgctccgg gcgtctgcgc gcgagccgac gctggcgcag
     3661 cagctcgatg cgctgctgac cgatgtcgag ttccacaagc tgatcgaggc cgtcaccgcc
     3721 gaggtcgtgc ggagatggaa cgtgccgcgc cgcaccgccg cggggctcgt gatgtcggcg
     3781 gtcggagagc ccgggacgct gcactgcgtg catcgcgcgt ggttcctggc cgagacccgc
     3841 ggcagcggcg cccggctcgc gaagctgatc gtccggcgcc gcgtgttcga cctgctgcgc
     3901 gccgatgccc ggcgcccgct gcacacgtcg ttgccgcgca ccagcgacga gatcgacggt
     3961 gcgctgcgcg cgctggacgg ccggatcgac cgggattcgc acgcgcaggc ggagctccac
     4021 gcggtggcga gccgggtccg cgcggcgctg gagtgcttcg cgcggcaggg gccgtgccag
     4081 gcgcgccagg catgcctggt gcgccgccat gcgctcgacg agatcgatcg gctcgatctc
     4141 gccgcgcagc tggcgtgctc gcagaacgcg ctgcgcgtgc gcatccacaa ggcgctgcgc
     4201 gcgctgcgca agcacatcga ggcgtgtcac c
//
LOCUS       DASVOR010001993         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488765,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001993 DASVOR010000000
VERSION     DASVOR010001993.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488765"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..543
                     /locus_tag="VF516_37025"
     CDS             184..543
                     /locus_tag="VF516_37025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113410.1"
                     /translation="MSAKSEEELDELTEETSDRREIYLREGRTLSVERAGQDELVEIR
                     SSSGQVELRIKLTEDGPVLQIESARLQLKASEAVEIESKRVEIRASETVQIASKDEVK
                     VDAEGDVRVNGKTIWLN"
     gene            635..>2071
                     /locus_tag="VF516_37030"
     CDS             635..>2071
                     /locus_tag="VF516_37030"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113411.1"
                     /translation="MDVGQAAPNLGAGRFEVRGTLGAGGAGVVYRAFDRQLQREVALK
                     LLRQASGRDLYRFKKEFRSLADIVHPNLVALHELHAADGDWYFTMELVEGVSFIDWVR
                     PSAEIGPGRARADIVSARVDLVRLHSALVQLVDALIALHRAGKLHRDLKPSNVLVTRT
                     GRLTLLDFGLVASVAEGDPERLAVGTPVYMSPEQAADRPLSEASDWYSLGAMLYEALV
                     GRRPFEGESEQVMTRKQTEQPPSPRQLDPGVPAELSQLCMMLLQPDPAARPSGLMVLE
                     ALGAAPSETTRTIGRSYPPAMFVGRTAEMEVLRRALGDARRGGVAMLVRGRSGIGKTT
                     LVRRFLRSLGDSVFVLEGRCFEREAVPFKMLDGVVDSLTGAIVGLPLLIAEQLVPRDL
                     GSLVRLFPVLRRVPKFAELAAQSPAPPDPQEMRRRGFTALRSVLGRLARMRSLVIFVD
                     DAHWGDADSAVFLSELIHGAEPGTLILVA"
BASE COUNT          286 a          698 c          789 g          298 t
ORIGIN      
        1 ctgctccgcg cgctccgtcg accggcggcg ccgccgcccc cgttgcgcgt gcccaggacc
       61 tgggcgctgc gccagctcga ggcgtgggtc caggaaaacc actgagccgg agcggcgttc
      121 agccgtgccc gcgccagggg agcctgggcg gggctgcgcc gtaccatgtg ataccttcca
      181 gtcatgtcgg cgaaatccga ggaagaacta gacgagctga ccgaggagac gtccgaccgg
      241 cgtgagatct acctgcggga aggccgcacg ctgagcgtcg agcgggcggg gcaggacgag
      301 ctggtggaga tccgcagctc gtcgggccag gtcgagctcc gcatcaagct gaccgaggac
      361 ggcccggtgc tgcagatcga gagcgcgcgg ctccagctca aggcgagcga ggcggtcgag
      421 atcgagtcca agcgcgtcga gatccgggcg agcgagaccg tgcagatcgc gagcaaggac
      481 gaggtcaagg tcgacgccga gggcgacgtc cgggtcaacg gcaagacgat ctggctcaac
      541 taggcgcacc ggcccggaat cggatcgcct cgccccgatt cccctgttcc attcaacgtt
      601 tcggtgaaac gagagtgata tcctggagct ctcgatggac gtcggtcaag cggcgccaaa
      661 cctcggggcc ggtcgcttcg aggtgcgcgg caccctgggt gccggcggcg ccggggtcgt
      721 ataccgcgcg ttcgaccgcc agctgcagcg cgaggtcgcg ctcaagctgc tgcgccaggc
      781 gtccggccgc gatctgtatc gcttcaagaa ggagttccgc tcgctcgccg acatcgtcca
      841 ccccaacctg gtggcgctgc acgagttgca cgcggccgac ggcgactggt acttcacgat
      901 ggagctggtc gagggcgtgt cgttcatcga ctgggtccgg ccgtcggccg agatcgggcc
      961 gggccgggcg cgcgccgaca tcgtgtccgc gcgggtcgat ctggtgcggc tgcacagcgc
     1021 gctggtccag ctggtcgatg cgctgatcgc gctacaccgc gccggcaagc tgcaccgcga
     1081 tctcaagccg tcgaacgtgc tggtgacccg gaccggacgg ctgacgctgc tcgacttcgg
     1141 cctggtcgcg agcgtggccg agggcgatcc cgagcggctc gcggtcggca cgccggtcta
     1201 catgtcgccc gagcaggcgg ccgatcggcc gctgagcgag gccagcgact ggtactcgct
     1261 gggcgcgatg ctgtacgagg cgctggtcgg ccgccggccg ttcgagggcg agtccgagca
     1321 ggtgatgacc cgcaagcaga ccgagcagcc gccgtcgccg cgccagctcg acccgggcgt
     1381 gccggccgag ctgtcgcagc tgtgcatgat gctgctccag cccgacccgg cggcgcggcc
     1441 gagcgggctg atggtgctcg aggcgctggg cgcggcgcca tcggagacca cccggacgat
     1501 cggccgcagc tacccgccgg cgatgttcgt cggccggacc gcggagatgg aggtgctgcg
     1561 gcgcgcgctc ggcgacgcgc ggcgcggcgg ggtcgcgatg ctggtgcgcg ggcggagcgg
     1621 gatcggcaag accacgctgg tccgccggtt cctgcgcagc ctgggcgact cggtgttcgt
     1681 gctggagggc cggtgcttcg agcgcgaggc ggtgccgttc aagatgctcg acggcgtggt
     1741 cgactcgctg accggggcga tcgtcgggct gccgctgctg atcgccgagc agctggtgcc
     1801 gcgcgacctc ggctcgctgg tccggctgtt cccggtgctg cgccgggtgc ccaagttcgc
     1861 cgagctcgcc gcgcagagcc cggcgccgcc cgacccgcag gagatgcggc gccgcgggtt
     1921 caccgcgctg cgcagcgtgc tcggccggct ggcgcggatg cggtcgctgg tgatcttcgt
     1981 cgacgacgcg cactggggcg acgccgacag cgcggtgttc ctgtccgagc tgatccacgg
     2041 cgccgagccc gggacgctga tcctggtcgc c
//
LOCUS       DASVOR010001994         4794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_196558,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001994 DASVOR010000000
VERSION     DASVOR010001994.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196558"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1256
                     /locus_tag="VF516_37035"
     CDS             234..1256
                     /locus_tag="VF516_37035"
                     /inference="COORDINATES: protein motif:HMM:NF013706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEX8113412.1"
                     /translation="METDRRDFLKAAGAAGAIAAAGATLGGSGSASAAPARPGPKGMA
                     RGLTLLTMRQGNDHRLGVKTDHGILDVKAATGLLHLPAPATMDDLLQNEDGPNLNALV
                     DAALKASSAKKAFLQEDAIEYGPVVTRPEKIVCIGLNYKRHAKEVNQPIPKQPILFNK
                     FNNALNAHKGKIKLPVEVATKFDYETELVIVIGKEARNVSEADATGYIAGFATGNDFS
                     ARDLQLESGGQWMIGKTVDQFGPLGPYLVTADQVDGDNLKLETWVNGELRQNSNTNDL
                     IFNTKQLVSFISRHLTLRPGDIIYTGTPEGVIQGKPADKRVWLKAGDKIVSSLEKLGK
                     LEFELV"
     gene            1288..1998
                     /locus_tag="VF516_37040"
     CDS             1288..1998
                     /locus_tag="VF516_37040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113413.1"
                     /translation="MRRRSLLLAIALPVAAAACGSNEHPAIDASPAADAPAPDAPTCP
                     TQLLTGGTDVGPQGWSVIMQPPATLTNGPDYVKLQTSTTTNATVSGQLLLTYPGAVEM
                     GKLFKIQVVMQVDAVNSHNPLDSAAAILGSFTAQTGFGTTAERNQMIYLDAGKIGWAD
                     DSPSFPSFPFMVTDSAYHTYELSVDASGNATVTIDGTQALTRKGFTTNGAIAIGDQTN
                     EPNVDSTLRIRSVTRLCL"
     gene            complement(2006..3745)
                     /locus_tag="VF516_37045"
     CDS             complement(2006..3745)
                     /locus_tag="VF516_37045"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8113414.1"
                     /translation="MNAASARLIETIRAAIIGADHALDGPYGPRRVTYADYTASGRSL
                     DFIEDFLRDEVMPLYANTHTESSGTGRQTTAFREDARAIIHCAVGGGPDDLVIFTGSG
                     ATGAINKLLDILNLRIPRDLDTRYGLSRQIPLDERPVVFIGPFEHHSNELPWRESIAE
                     VCVIREDADGHIDLDHLERELVRHAARPLKIGSFSAASNVTGIASDTRAIAALLHRHG
                     AYSFWDYAAAGPYVAIEMNGGAAGDGADKDAVFISPHKFIGGPGTPGVLVAKRHLLRN
                     TVPACPGGGTVAYVNEAEHRYLTDPVHREEGGTPAIIESIRAGLVFQLKEAVGAEAIR
                     ERELELLQRAIASWSTNPNLTVLGNRHAFRLSIVSFVVRHRTAYLHHNFVVTLLNDLF
                     GIQARGGCSCAGPYGHRLLGIDLDTSKEFEREIVRGCEGIKPGWVRVNFNYFLSETEF
                     SYLVDAIHLVANDGWRLLPHYDFDPDSGQWRHRSRRGHDVRRLTDVSYRCGRLEFRSR
                     HASEPEAALLGYLDEARRIIAATHTEYAGLELAERPMTQDFEELRWFPLPGEVLAELR
                     GRGGTSPSVVLHP"
BASE COUNT          791 a         1659 c         1647 g          697 t
ORIGIN      
        1 gccgctccgc gcgctcggcc acgacgacgc cgcgctgttc gacgcctgcg ccgcggcgag
       61 ctcgttcggc gtgttctcgc ggatccgcgt cgcgctgacc gcgctcggcg gcggaccggg
      121 cgcggccatc tgaacaatcc gttccatcgg cgacccctca aaaaaacgtc gattgaatgt
      181 ccgccatccc cgtgcgagcg tcggcggcga tcgaccagac acggaggaca acgatggaga
      241 ctgatcggag agatttcctc aaggcagccg gggcggcagg tgccatcgcc gccgcgggtg
      301 ccacgctggg cggctcgggg agcgcgtcgg cggcgccggc tcgcccgggt cccaagggca
      361 tggcccgcgg cctcaccctc ttgacgatgc gccagggcaa cgaccaccgg ctcggcgtca
      421 agaccgatca cggcatcctc gacgtcaagg ccgccaccgg cctgctccac ctcccggcgc
      481 ccgcgacgat ggacgacctg ctgcagaacg aggacggccc caacctcaac gcgctggtcg
      541 acgccgcgct caaggcgagc tcggccaaga aggcattcct ccaggaggac gccatcgagt
      601 acgggccggt ggtcacccgt ccggagaaga tcgtttgtat cggactcaac tacaagcggc
      661 acgccaagga ggtcaaccag cccattccga agcagcccat cctgttcaac aagttcaaca
      721 acgcgctcaa tgcccacaag ggcaagatca agctgccggt cgaggtcgcg accaagttcg
      781 attacgagac cgagctggtg atcgtgatcg gcaaggaagc caggaacgtc agcgaggccg
      841 acgccaccgg ctacatcgcc gggttcgcca ccggcaacga cttcagcgcg cgcgatctcc
      901 agctcgagag cggcggccag tggatgatcg gcaagactgt ggatcagttc gggccgctcg
      961 ggccgtacct ggtgaccgcg gaccaggtcg acggcgacaa cctcaagctg gagacctggg
     1021 tcaacggcga gctgcgccag aactcgaaca ccaacgacct gatcttcaat accaagcagc
     1081 tggtcagctt catctcgcgc cacctgacgc tgcgcccggg cgacatcatc tacaccggca
     1141 cgccggaggg cgtgatccag ggcaagcccg ccgacaagcg ggtctggctc aaggccggcg
     1201 acaagatcgt gtcgagcctg gagaagctcg gcaagctgga gttcgagctg gtgtgaagcc
     1261 ggggtgaagc cggtgtagag tgggcgggtg cgccgccgat cgctcctgct cgccatcgct
     1321 ctccccgtcg cggcggccgc ctgcgggtcg aacgagcatc cggcgatcga tgccagcccg
     1381 gcggccgatg ccccggcgcc cgatgccccg acgtgcccga cccagctgct gaccggcggc
     1441 accgacgtcg gaccgcaggg ctggtcggtc atcatgcagc caccggcgac actgaccaac
     1501 ggcccggact acgtgaagct gcagacctcg accaccacca acgcgaccgt cagcggccag
     1561 ctgttgctca cctaccccgg cgcggtagag atgggcaagc tgttcaagat ccaggtcgtg
     1621 atgcaggttg acgcggtgaa ctcgcacaac ccgctcgaca gcgccgcggc gatcctcgga
     1681 tcattcacag cccagaccgg cttcgggacc accgccgagc gcaaccagat gatctacctc
     1741 gacgccggca agatcggctg ggccgacgac agcccgtcgt tcccgtcgtt tccgttcatg
     1801 gtgaccgaca gcgcgtacca cacctacgag ctgtcggtcg acgcctccgg caacgcgacc
     1861 gtcaccatcg acggcaccca ggcgctgacc cgcaagggct tcacgaccaa cggcgcgatc
     1921 gcgattggcg atcagaccaa cgagccgaac gtcgacagca cgctgcgcat ccgctcggtg
     1981 acccggctct gcctgtgacc cgcggtcacg ggtgcagcac gaccgacggc gacgtgccgc
     2041 cgcggccgcg cagctcggcg agcacctcgc ccggcagcgg gaaccagcgc agctcctcga
     2101 agtcctgcgt catcggccgc tcggcgagct ccagcccggc gtactcggtg tgggtcgccg
     2161 cgatgatccg gcgcgcctcg tcgaggtagc ccagcagcgc ggcctccggc tcgctggcgt
     2221 ggcgcgaccg gaactccagc cgcccgcagc ggtacgacac gtcggtcagc cggcggacgt
     2281 cgtggccgcg ccgcgagcgg tgccgccatt gcccggagtc gggatcgaag tcgtagtgcg
     2341 gcaggagccg ccagccgtcg ttggcgacga ggtggatcgc gtcgaccagg tacgagaact
     2401 cggtctccga caggaagtag ttgaagttga cccggaccca gcccggcttg atgccctcgc
     2461 agccgcgcac gatctcgcgc tcgaactcct tgctggtgtc gaggtcgatg ccgagcagcc
     2521 ggtggccgta cggcccggcg cacgagcagc cgccgcgggc ctggatcccg aacaggtcgt
     2581 tgagcagcgt gaccacgaag ttgtggtgga ggtacgccgt gcggtggcgc accacgaacg
     2641 acacgatcga cagccggaac gcgtggcggt tgccgagcac cgtgaggttg gggttggtcg
     2701 accagctcgc gatcgcgcgc tgcagcagct cgagctcgcg ctcgcggatc gcctcggcgc
     2761 cgaccgcctc cttgagctgg aacaccagcc cggcgcggat cgactcgatg atcgccggcg
     2821 tgccgccctc ctcgcggtgc accggatcgg tgaggtagcg gtgctcggcc tcgttgacgt
     2881 acgccaccgt gccgccgccc gggcacgccg ggacggtgtt gcgcaacagg tggcgcttgg
     2941 cgaccagcac ccctggggtg cccggcccgc cgatgaactt gtgcggcgag atgaacactg
     3001 cgtccttgtc cgcgccatcg ccggccgcgc cgccgttcat ctcgatcgcc acgtaggggc
     3061 cggcggccgc gtagtcccag aacgagtacg cgccatggcg gtgcagcagc gccgcgatcg
     3121 cgcgggtgtc gctcgcgatc ccggtgacgt tcgacgccgc cgagaagctg ccgatcttga
     3181 gcggccgggc ggcgtgccgc accagctcgc gctcgaggtg gtcgagatcg atgtgcccgt
     3241 cggcgtcctc gcggatcacg cagacctcgg cgatcgactc gcgccacggc agctcgttgg
     3301 agtggtgctc gaacgggccg atgaacacga ccggccgctc gtcgagcggg atctggcgcg
     3361 acaggccgta ccgggtgtcg aggtcgcgcg ggatccgcag gttgaggatg tcgagcagct
     3421 tgttgatcgc gccggtcgcc cccgagccgg tgaagatgac gaggtcgtcg ggcccgccgc
     3481 cgaccgcgca gtggatgatc gcgcgggcgt cctcgcggaa cgccgtggtc tgccggccgg
     3541 tgcccgagct ctcggtgtgc gtgttggcgt agagcggcat cacctcgtcg cgcaggaagt
     3601 cctcgatgaa gtcgagcgag cgtcccgacg cggtgtaatc cgcatacgtg acgcggcgcg
     3661 gcccgtacgg cccgtcgagc gcatgatcag cgccgatgat cgcggcgcgg atggtctcga
     3721 tcaggcgtgc actggcggcg ttcattcagt gaatctatca cctcgccgcc ggcgttcact
     3781 gcgacatgtg cgtcgagctc ctcgacgaac tgccgctcag cgtcgctggg cgcgcagtgc
     3841 atccaggatg agcctgcatc cgagccgcac ctcgatgagt gacgaggttc actgcatgtg
     3901 taaagacact ggagacctct ggctcggctc cgtccacgtg cgctgttcct ccgtcatggt
     3961 ggatccgtga acacggaagg atcgctccgc ggggagaccg ccgccgcgcg ccctggcaca
     4021 cctcgactgc aggcgggccg tgtcgcgcag cctcgtcaag ccgatcggac gatgctcgtt
     4081 ccaggacggc tgcgccggtg aacgcgcact gaggccgtcc agtcattctt cgtcgtcgtt
     4141 catggtcgct cctgcgatcg cagccagcgc ctcttcgagc gcagggatca cggcgcgatc
     4201 ctagagccga tcgtcatcac cccgcacgca gcgctggcag gcaccgaggc ggcagccatg
     4261 aggtcgcagg ccgggcgatt ccgcggaatc gtgtcggcgc ctccgtgact ggcacggcga
     4321 gcgcggtgca gccggcatgc cgcgccggca gtggcagcca tgacggaggg tcggctgcga
     4381 agatgcagcc ggagaccggc acaggctggc acccacgtgg gggcgttgcc gtccgcggtt
     4441 ggctgcgcgg tgcccggacg attccgcaga atcgtgtcgg ggcctcggcg cctggcacgg
     4501 cgagtgcggt ctggggagtg tgtagcgcgt ggcaaccgga gaacgctcac acgcgaactc
     4561 gccgtgggct gctgcgcgga tgggtgtcgg agccgcgccc gccgaatcac caccgaccca
     4621 tgcgcggacg gcgctaggta tgtgcggaca acacccggcc aatccagatc acgggtcggg
     4681 tagcccggcg acttgggctc gccgggcccc cacagatccg gccaagcgga attcccgcag
     4741 ccggctcttc ggagaacagg ttcgctaggt tccgtaccgg tgaacgatgc gcgg
//
LOCUS       DASVOR010001995         1504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_459267,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001995 DASVOR010000000
VERSION     DASVOR010001995.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459267"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="VF516_37050"
     CDS             complement(<1..822)
                     /locus_tag="VF516_37050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113415.1"
                     /translation="MEGSRHGLTRELALAIWKRVRDEATDEQGRIDDAQARRRFHELA
                     ARIAIGGGRLWPDVGRRTRVDLEDGGPTAATRTGGRLPWVPGRTTLVAAEEQRWAALD
                     ARHALASAVPGRQTLVAAELHIGTTADARPPVPTEAIPAPEPALPARSPLHPRSSEQA
                     RARPARRDEPRGVTGPRDLDLASAASYLHAHTLCDAIYQALVDDHVLEEERLRAFFGD
                     VEPDLLRTALALLRGRTVAGIGAFTLLDLTMRGSLGRFLGASALTVGATDAAEERE"
     gene            complement(1298..>1504)
                     /locus_tag="VF516_37055"
     CDS             complement(1298..>1504)
                     /locus_tag="VF516_37055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113416.1"
                     /translation="LLFDVYGAPKYSTGPYGAISLLGLDYDLGQAVRIVFDPMEIALP
                     VPLLGQLPLYYEQFRFVIGVQFGA"
BASE COUNT          220 a          537 c          527 g          220 t
ORIGIN      
        1 ctcgcgctcc tcggcggcat ccgtggcgcc gaccgtcagc gcggaggccc cgaggaaccg
       61 cccgagcgag ccccgcatcg tcaggtcgag cagcgtgaag gcaccgatcc cggcgaccgt
      121 gcggcctcgc agcagcgcga gcgccgtgcg cagcaggtcg ggctccacgt cgccgaagaa
      181 cgcgcgcaag cgctcctcct cgagcacgtg atcgtcgacc agcgcctggt agatcgcgtc
      241 gcacagcgtg tgggcgtgga ggtagctcgc cgcgctggcg agatcgagat ctcgcggccc
      301 ggtaaccccg cgcggctcgt cgcgtctcgc cgggcgggcc cgcgcctgct cgctgctccg
      361 aggatgcagc ggcgatcgag cggggagcgc aggctcgggc gcggggatgg cctccgtagg
      421 gacggggggc ctcgcgtcgg cggtcgtccc gatgtgcagc tcggcagcaa ccagcgtctg
      481 gcgaccgggg acagcgctgg ccagcgcgtg acgcgcatcg agggctgccc accgctgttc
      541 ctcggcagcg accagcgtgg tgcggcccgg tacccagggg agccgcccgc cggtgcgggt
      601 cgccgcggtc gggccgccgt cctcgaggtc gacgcgcgtc cgcctgccga catcgggcca
      661 cagccggcca ccgccgatgg cgatgcgagc cgcgagctcg tggaatcgcc gccgtgcttg
      721 cgcatcatcg atccggcctt gctcgtcggt cgcttcgtcg cggacccgct tccagatcgc
      781 cagcgccaac tcccgcgtca ggccatggcg ggagccttcc accgctggat cgaatcgatc
      841 tctcgcgcgc tggcgttcga gctcaggtgg atagatccag cgcgtcgtca tctccctacc
      901 ccctggagcg tgtcgccgac ggtggtagcg ctctgcggat cgatgcacct cccccgaccc
      961 gtcggaggtt cccgcgcgcc ctgccggcgg tcgcccgcga tggtcgacgg gagcctatcc
     1021 gagtgcgcag gaagagtagc agcgcgatcc tggcaaacgt tgcgcgagac gcgaccagcg
     1081 gacgtggcga gacgcaacca atgtatgtcg cagcacggtg ctttcctact ctcgcgatca
     1141 tcatggctgc gtccgtagct cggtcgatgg gccgtcacgt cacgcggtgt acggtgggct
     1201 cgccgcaatc cacccgctgc cccgtcgaat tgacgggtct gctgcaaatc tcgcaagaac
     1261 cgtcagcccg ccgtggactc cccagtctcg ggaatggtca ggcgccgaac tggacgccga
     1321 tcacgaaccg gaactgctcg tagtacagcg gcagctgacc gagcagcggc accggcagcg
     1381 cgatctccat cgggtcgaac acgatccgca ccgcctggcc gagatcgtag tcgagcccga
     1441 gcaggctgat cgcgccgtag gggccggtgc tgtacttcgg cgcgccgtag acgtcgaaca
     1501 gcag
//
LOCUS       DASVOR010001996         3860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_225772,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001996 DASVOR010000000
VERSION     DASVOR010001996.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225772"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VF516_37060"
     CDS             <1..318
                     /locus_tag="VF516_37060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113417.1"
                     /translation="IADLGAHGLQLPQPSCRNRRSAESGPVPHEADPGQRLLHAGDEA
                     VDAAVPGHGGQRVGVPARLAEHGAHAGSPGGGIALIPDLQIALSDDESLTTSPAAATR
                     TLQ"
     gene            760..2145
                     /locus_tag="VF516_37065"
     CDS             760..2145
                     /locus_tag="VF516_37065"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8113418.1"
                     /translation="MEIAQVIAGKYRVDGLLGRGSTSVVVRATHLQLHQPVAIKFLLS
                     ELRRHRPLAQRFLREAQAAVRLRSEHVARVLDVGATEDDTPYMVLEYLDGADLESFPR
                     SQLTVGSIVDLILQACEALSEAHALGIVHRDIKPANFFVTRYTDGALLLKLLDFGISK
                     IDTTGERLTAPQTTMGTPAYMSPEQMRSPHDVDHRSDIWSLGVVLYELLQGALPFWAD
                     TLSSLVLKVVNEPLPKLTVRLPGDLDAIVYRCLEKDPARRFQNAAELAFALAKFARSE
                     SQAAISVQRTRGILEALEVRRGTRAPPVAPRARFSTGRTSAGDRTLPLHGDRRWPITA
                     GLGSLAVVLLISLMAASWRDGADDRGSSPALGHNASAPATPQPSERPPVAPPALVASM
                     PPAARLPAALDAPSPTAAAPLAPCAGTAPASEMARKPRPVMKLSESPRARTTPVRPPA
                     LPDDAILEKRK"
     gene            2243..3253
                     /locus_tag="VF516_37070"
     CDS             2243..3253
                     /locus_tag="VF516_37070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113419.1"
                     /translation="MLAAGARTAHSEPDSTAAIAEQLFDEGRVLMRAGRWAQACPRFD
                     ASQRIDPALGAQLNLAACYERVGRLARAWNLYREAIDLARKADDVTRRDYAQRHAAAL
                     EPRLARLTITVPTKLPAGFAVRWDGSPIPPNALGTGLYADPGQHEILVVAPGFETVTK
                     TVALVGGTAKIVAIPVLVGSAAPGYEPSTLSSTGPTPDLVVASHRASVLPAPTRPDDP
                     PSSRRYLAIGLGTAGLATAGVGLWFGMKTRSSYQQAKELCGEQLICDNAADFETGKRL
                     IRDARSDAITSTLLVASGGAVVVGGVIVLLVTSRTGERASAQIVPVLNDHGAGLAVVG
                     RL"
     gene            3259..3741
                     /locus_tag="VF516_37075"
     CDS             3259..3741
                     /locus_tag="VF516_37075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113420.1"
                     /translation="MHKTLLSVSLWMLPTGCAALFGLKDVQIVDGGSGHSTDGMADSA
                     TTDGAMTDSAMMDGAMMDSAMMDSAMTDRMYHCGYAAPGKCDNRRRSQPLIAPDMTAA
                     IAACKKQKPKPDLDFCYVKDDDGMAATDESQCMAASGSWRPDRNCCAFMGTPSCSASP
                     "
BASE COUNT          670 a         1301 c         1290 g          599 t
ORIGIN      
        1 attgccgatc tgggtgctca cggcctgcag ctcccgcagc cttcctgcag gaaccgcaga
       61 tctgcggaaa gcggcccggt cccgcacgaa gcggaccccg gccaacggct gctgcacgcc
      121 ggcgatgaag cggtcgatgc cgccgtgccc ggccacggcg gccagcgggt cggtgtacct
      181 gcacgcctcg ctgaacacgg cgcgcacgcg ggctcgccgg gcggcgggat cgcgctgatt
      241 ccagatctgc agatagcgct ctctgacgac gaatccctca ccacgtcgcc ggccgccgcg
      301 acacgaacat tgcagtaaat tgccacacgg atgtcatatg cctgcacatt gtgctgaatc
      361 accgggtgac atcacacaat ggcttcgcat ggccgtgatg cctgtcgaga caacgagcgc
      421 tggcgcacgg tggcgccctg caacgtctcg accgatggtc atgaccggtg agtcgcagaa
      481 aacctcggca gtggcgtcgg atacacctgg tggacaacgc acgaactgct gatatggtga
      541 ttgctgaggc caggaatcgc ggagggaggc cggccaactc atcgactgat tgatggtctg
      601 acttaggcag gtgtcaggga gcagcgagcc acaaccaggg ggtcagatgc cggcttggat
      661 cgacaggacc gcagcaacgc gtggcgatgg attcgcccgg cccgcgatcg ctgtcgtggt
      721 gtcaggtgat gggtcccggg ggtcgtcgtg acggtcgcca tggagatcgc ccaggtcatc
      781 gcgggcaagt atcgcgtcga tggtttgctc ggtcggggca gcacgagcgt ggtcgtgcgg
      841 gccacgcacc tccagcttca tcagccggtg gcgatcaagt tcctgctgtc cgagctgcgc
      901 agacaccgtc cgctcgccca gcgcttcttg cgcgaagcac aagcggccgt ccggctgagg
      961 agcgagcacg tggcccgcgt gctcgacgtc ggcgcgacgg aggacgacac accgtacatg
     1021 gtgctggagt acctcgacgg cgccgacctg gagagctttc ctcgatccca gctgacggtc
     1081 ggcagcatcg tcgacctgat cctgcaggcg tgcgaagcgc tgtccgaagc ccatgcgctc
     1141 ggtatcgtgc atcgcgacat caagccggcg aacttcttcg tcacgcggta caccgacggc
     1201 gcgctgctcc tgaagctgct cgacttcggg atctcgaaga tcgatacgac cggagagcgg
     1261 ctcaccgctc cccagaccac gatgggcact ccggcgtaca tgtcaccgga gcagatgcgc
     1321 tcgccgcacg acgtcgacca tcgcagcgac atctggtcgc tgggggtcgt gctctacgag
     1381 ctgttgcagg gtgcgctgcc gttctgggcc gatacgttgt catcgctggt cctcaaggtc
     1441 gtgaacgagc cgttgcccaa gctcacggtt cgcctgccgg gagatctcga tgccatcgtg
     1501 tatcgctgtc tcgagaagga cccggcgagg cggtttcaga acgccgccga gctcgccttc
     1561 gcgctcgcga agttcgctcg ctcggagtca caagcagcga tctcggtgca acgcacacgg
     1621 gggatcctcg aagcgctcga ggtaaggcgg ggaacacgcg cgccgccggt tgccccacgc
     1681 gcacgattct cgacgggccg gacctctgca ggcgaccgaa cgctgccgct ccacggcgac
     1741 cggcgctggc cgatcacggc cggcctgggg agcctggccg tggtcctcct gatctcgctc
     1801 atggcagcga gctggcgcga cggagccgac gatcgggggt cgagtcctgc gctcggtcac
     1861 aacgcgtccg cgccggcgac gccgcaaccg agcgagcgtc cgccggtcgc gccgcccgcc
     1921 ctggttgcca gcatgccgcc cgcggctcgc ctcccggccg cgctggacgc accgtcgccg
     1981 acagcggcgg ccccactggc accgtgtgcc gggacagccc ccgcgagcga gatggccagg
     2041 aagccgaggc cggtgatgaa actctccgag tcgccgaggg ccagaacgac gcctgtcaga
     2101 ccgccggcac tgccggacga cgcgatcctc gaaaagcgca agtgacatga tgaccccagc
     2161 gtcccgtcga cgacctagcg cgcgaaccgg ccgaccggtg aggtacgcga cgatcccgcg
     2221 atgcctgctc ggactggcag tcatgctggc ggccggggcc aggaccgcac attccgagcc
     2281 ggacagcaca gcggccatcg cggagcagct gttcgacgag ggccgcgtgc tcatgagggc
     2341 ggggcgctgg gcgcaggcgt gtccgaggtt cgacgccagc cagcgcatcg atccagcgct
     2401 tggagcgcag ctcaacctgg ccgcgtgcta cgagcgagtg ggcaggctgg cgcgagcctg
     2461 gaacctctac cgtgaagcga tcgacctcgc ccgaaaggcc gacgacgtca cgcggcgcga
     2521 ctatgcgcag cgccatgccg cggcgctcga gcccaggctc gccagactca cgatcaccgt
     2581 tccaaccaag ctgcccgccg gattcgctgt ccgatgggac ggcagtccga tcccgccgaa
     2641 cgcgcttggg accggcctgt acgccgaccc gggacagcac gagatcctcg tcgtggcacc
     2701 cgggttcgag accgtcacga agaccgtcgc gctggtcgga ggcacggcca agatcgtcgc
     2761 gatcccggtc ctcgtcggat cggcggcccc aggatacgag ccctcgacgc tctcatccac
     2821 gggtccaacg cctgacctcg tggtcgcatc gcatcgcgcc tctgtccttc ccgccccgac
     2881 cagaccggac gatccgccgt cgtcacgacg ctacctcgcg atcggcctcg gcacagcagg
     2941 gctcgcgact gccggcgtcg gactctggtt tggaatgaag acgagatcca gctaccagca
     3001 ggcgaaggaa ctgtgcggtg agcagctgat ctgcgacaac gccgcagact tcgaaacagg
     3061 caagcgattg attcgagacg cccgatccga cgccatcacc tccacgctgc tggtcgcttc
     3121 cggcggcgcc gtggtcgtcg gaggtgtcat cgtgctcctg gtcacctccc ggacgggaga
     3181 acgggcgagt gcgcagatcg taccggtgct caatgaccac ggcgcagggc tcgctgtcgt
     3241 cgggaggctc tagctcacat gcacaagacc ctgttgtccg tgtcgctatg gatgctaccc
     3301 acgggctgcg ctgccctctt cggtctgaaa gacgtccaga tcgtggatgg cggcagtggt
     3361 cacagcaccg atggcatggc ggatagcgcc acgacggatg gtgccatgac ggatagcgcc
     3421 atgatggatg gcgccatgat ggatagcgcc atgatggata gtgccatgac ggaccgcatg
     3481 taccattgcg gttatgccgc tccaggaaag tgtgacaacc ggcgccgcag tcagcccctc
     3541 atcgcgcccg acatgacggc ggcgatcgcc gcatgcaaga agcagaagcc gaagccggac
     3601 ctggacttct gctacgtgaa agacgacgac ggcatggctg ccacggacga gagtcagtgc
     3661 atggcggcct cgggctcctg gcgaccggac aggaactgct gcgccttcat gggaacgccg
     3721 agctgctcgg ctagcccttg aacgtcgacc ggccgacgtc gccgaacggc tcgtcgcgct
     3781 cgcgcaccgc ctgcttgaag ccgacctgct cggcgcgctg ctggaacgcg tagccctcgg
     3841 cggtgtggcg ggtgatgccg
//
LOCUS       DASVOR010001997         4053 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_50739,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001997 DASVOR010000000
VERSION     DASVOR010001997.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50739"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VF516_37080"
     CDS             complement(<1..478)
                     /locus_tag="VF516_37080"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8113421.1"
                     /translation="MPFFDLRGLATHFAVDGPPGAPALLLVHSLGTSLRVWDAQAEAL
                     ARGFRVVRFDLRGHGLTEVGAGPCTVEDLADDALALLDALGIASAHVAGVSIGGMVSQ
                     AIAARAPARVRSLILCDTAMAIPPADLWRQRAAQVRAEGIAAIADTVLARWASPAYL"
     gene            complement(536..895)
                     /locus_tag="VF516_37085"
     CDS             complement(536..895)
                     /locus_tag="VF516_37085"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113422.1"
                     /translation="MDDRPDVAKAIARMLSNHDTATECDPRQAVARVEHGERFDIVLC
                     DLNMPDMNGREVSDRLVHAQPDPPPIVLMMSGGENVDSLFATGRAVLIKPFDGKELRE
                     LVSAMLHEADWTAHHHA"
     gene            complement(1084..1641)
                     /locus_tag="VF516_37090"
     CDS             complement(1084..1641)
                     /locus_tag="VF516_37090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113423.1"
                     /translation="MATVGSYLLAIVGFGMMVSAVAGTLGSNLKYIYTRPLLINALRT
                     NGNYAEQLCKTRPDSYYGAIGAAIKTAAMCQSRDPKVVTGATVPSYDAQAMAVSMKWK
                     QLLGRVKLGLMAAGGAVVIGLSKGVPPILVIVLAVGVGIGFLWLYFFKAEIDRCMILA
                     RAEILPEVDRAFVDGRYVFPPNPPG"
     gene            1891..>4053
                     /locus_tag="VF516_37095"
     CDS             1891..>4053
                     /locus_tag="VF516_37095"
                     /inference="COORDINATES: protein motif:HMM:NF012211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEX8113424.1"
                     /translation="MVGNMNFAQVCGVVTCAVTAFWGCGDDREATSTSNGTPATQAAA
                     GAPPGASRAADRSATTQMGTPATIELIADPHAAQTGARVVTGYSQPAHGRITLIGPRA
                     IYAPTPGYTGSDSFQYTVTDAGGQTSTATARVIVAATTPSCTITISGPSSAMIGPAIH
                     LTATADCNISTPEIQWQHRVGNTGTFSTFKNFSTATSADFTTLGQALGLHQFRARVRA
                     QGTTTVFLSNTLGTTLVANTTPCTSVVLDTPAAGAVFGVGQAIALHATATCPAGVVPE
                     FQYLAMQPTDPGFTALPGTFPGGSSYTPPQPGSWVLAAAVRASGSTDPFQLQSDPVTV
                     SVSHVPTAVDDSAVMNEDTTAMVNVLANDSDPDGDVLTATITTPPAAGTASISAGVIT
                     YHPAANYNGTDAIGYTVNDGHGNTASATVHVAINPVEDPPSAQHDFLTVAEDGSGTID
                     VTANDTDPDGDALTVVSFTQPSHGASSFTGNVATYTPTPGYTGSDELEYTISDGHGGT
                     STASVFVTVTATNDPPVAADDTLVTPEDTEGGVNLLGNDSDPDGDSLTVVAFTQPLHG
                     TVSVVAGLASYIPATDYNGPDAFGYTVADPSGATSTATVHVTVVPVNDPPVAIDDSAS
                     LDEDTTATIDAVANDSDVAGDVLAIASVTQPAHGTVAIVSGHEVTYTPAADYHGADSF
                     TYTIADPSGATATATVTLAIAAVNDPPVAVADATSLDED"
BASE COUNT          665 a         1445 c         1418 g          525 t
ORIGIN      
        1 cgaggtaggc gggtgacgcc cagcgcgcga ggacggtgtc ggcgatcgcg gcgatgccct
       61 cggcgcggac ctgcgcggcg cgctgccgcc acaggtcggc aggtgggatc gccatcgcgg
      121 tgtcgcacag gatcagcgag cggacgcgag ccggggcgcg cgcggcgatc gcctgcgaca
      181 ccatgccgcc gatcgagacg ccggcgacgt gggcgctcgc gatgcccagg gcgtcgagca
      241 gggcgagcgc gtcgtcggcg aggtcctcga cggtgcacgg tccggcgccg acctcggtca
      301 ggccgtggcc gcgcaggtcg aagcgcacga cgcggaagcc gcgcgccagc gcctcggcct
      361 gggcgtccca gacccggagg ctggtgccga gcgagtggac gagcaggagc gctggggcgc
      421 cgggcgggcc atcgaccgcg aagtgggtgg cgaggccgcg gaggtcgaag aacggcatgg
      481 gcgcgagggt agcgaaaagc accgcccgcg ggtgcctcgc cgtgccgtgg attgcctatg
      541 cgtggtggtg cgccgtccag tcggcctcgt gcagcatcgc cgagaccagc tcgcgcagct
      601 ccttgccgtc gaacggcttg atcagcaccg cgcggccggt cgcgaacaac gagtcgacgt
      661 tctcgccgcc cgacatcatg aggacgatcg gcggcggatc gggctgggca tggaccagcc
      721 gatccgagac ctcgcggccg ttcatgtccg gcatgttgag atcgcacagc acgatgtcga
      781 accgctcgcc gtgctcgacg cgcgcgaccg cctgccgcgg gtcgcactcc gtcgcggtgt
      841 cgtgattgct cagcatacgc gcgatcgcct ttgcgacgtc gggtcgatcg tccactacca
      901 agatcctcgc catgcaacct ccttcagatc ggtcgtcgaa gggccggtcc gcccgcagca
      961 tagcgccgcg ccgcggccgg gggcgccgaa aaacgcaccg gcgaacaacg catgagatca
     1021 ccccggcttg gccagagatc tgatcgccgg tcggacctat cggctgcgcg gggtgggtgt
     1081 gcgctagccc ggtgggttcg gcgggaacac gtagcggccg tccacgaagg cgcgatcgac
     1141 ctcgggcagg atctcggcgc gtgcgagaat catgcagcgg tcgatctcgg ccttgaagaa
     1201 gtacagccag agaaacccga tgccgacccc gaccgcgagc acgatcacca ggatcggcgg
     1261 gacgcccttg gacaggccga tcaccacggc gccgccggcg gccatcagac cgagcttgac
     1321 ccggccgagc agctgcttcc atttcatcga caccgccatc gcctgggcgt cgtacgacgg
     1381 caccgtggcg ccggtgacga ccttgggatc gcgcgactga cacatcgcag cggtcttgat
     1441 cgcggccccg atcgccccgt agtaggagtc cggccgggtc ttgcacagct gctccgcgta
     1501 gttgccgttg gtgcgcagcg cgttgatcag cagcggtcgg gtgtagatgt acttgaggtt
     1561 cgacccgagg gtgcctgcga ccgccgacac catcatgccg aaaccgacga tggcgagtag
     1621 atagctcccg accgtggcca cccggtcagc ttacgcgtct cgcgaggcga gttccagcgt
     1681 gatgacagtt gaccccagcg gacggtctgg ccagcccggc gtgctacccg cgcgccgcac
     1741 tcggcggccg cagttcggtc atttcgcaca tcgccgtcgt gaaattcgcc tccggctgga
     1801 ccgacgcggg ctcaccgtcc ccgattcagg tcatcgggag gggaacatta catgggtcat
     1861 actatcaatc ggtgactcga ttgccgtaac gtggtcggca atatgaactt cgcgcaggtc
     1921 tgtggagtag tgacgtgcgc ggtcacggcg ttctgggggt gcggggacga ccgcgaagca
     1981 acgagcacca gcaacggaac gccggccacc caggctgccg ccggcgcccc gcccggagcg
     2041 tcccgggcag ccgaccggtc cgccacgacg cagatgggca cgccggcgac gatcgagctg
     2101 atcgccgacc cgcacgcggc gcagaccggc gcgcgggtgg tcaccgggta cagccagcct
     2161 gcgcacggcc ggatcacgct gatcgggccg cgggcgatct acgcgccgac gccgggctac
     2221 accggcagcg acagcttcca gtacacggtc accgacgccg gcgggcagac gtcgacggcc
     2281 accgcccggg tcatcgtggc ggcgaccacg ccgtcgtgca ccatcacgat ctccgggccg
     2341 agctcggcga tgatcgggcc ggcgatccac ctgaccgcga cggcggactg caacatcagc
     2401 acgcccgaga tccagtggca gcaccgggtc ggcaacacgg ggacgttcag caccttcaag
     2461 aacttcagca cggcgacgtc ggccgacttc acgacccttg gccaggcgct cgggctgcac
     2521 cagttccggg cccgggtccg ggcgcagggc accacgaccg tgttcctgtc caacacgctc
     2581 gggaccacgc tggtcgcgaa caccacgccg tgcacctccg tggtgctcga taccccggcg
     2641 gcgggggcgg tgttcggggt cggccaggcg atcgcgctgc acgcgacggc gacgtgcccc
     2701 gccggcgtcg tgccggagtt ccagtacctg gcgatgcagc ccaccgaccc cggcttcacc
     2761 gcgctgccgg ggacgttccc gggcgggtcg agctacacgc cgccgcagcc cggcagctgg
     2821 gtgctggcgg ccgccgtgcg ggcgagcggc tccaccgatc cgttccagct ccagtccgac
     2881 ccggtgacgg tctcggtcag ccacgtgccg accgcggtcg acgacagcgc ggtgatgaac
     2941 gaggacacca ccgccatggt caacgtgctg gccaacgaca gcgatcccga cggcgatgtg
     3001 ctgaccgcga cgatcaccac gccgccggcc gccggcaccg cgagcatctc ggcgggcgtg
     3061 atcacctacc acccggcggc gaactacaac ggcaccgacg cgatcggcta cacggtcaac
     3121 gacggccacg gcaacaccgc gtccgccacg gtccacgtcg cgatcaaccc ggtcgaggat
     3181 ccgccgtcgg cgcagcacga cttcctgacc gtcgccgagg atggaagcgg cacgatcgac
     3241 gtcacggcca acgacaccga ccccgacggc gacgcgctga cggtggtgtc gttcacccag
     3301 ccgtcgcacg gcgcgtcgtc gttcaccggc aacgttgcga cctacacgcc gacgccgggc
     3361 tacaccggca gcgacgagct cgagtacacg atcagcgacg gtcacggcgg gacctcgacc
     3421 gcgagcgtgt tcgtcacggt gacggcgacg aacgacccgc cggtcgcggc cgatgacacg
     3481 ctggtcaccc cggaggacac cgagggcggc gtcaacctgc tcggcaacga cagcgatccc
     3541 gacggcgaca gcctgaccgt ggtcgcgttc acccagccgc tgcacggcac ggtgtcggtg
     3601 gtcgccgggc tggcgtcgta catcccggcg acggactaca acggcccgga cgcgttcggc
     3661 tacacggtgg ccgatccgtc gggcgcgacg tcgaccgcca cggtccacgt cacggtggtg
     3721 ccggtcaacg atccgccagt cgcgatcgac gacagcgcga gcctcgacga ggacaccacc
     3781 gcgacgatcg acgcggtggc caacgacagc gacgtcgccg gcgacgtgct ggcgatcgcc
     3841 agcgtgaccc agccggcgca cggcacggtc gcgatcgtct cgggtcacga ggtgacgtac
     3901 acgccggcgg ccgactacca cggcgcggac tcgttcacct acacgatcgc cgacccgagc
     3961 ggggcgacgg cgaccgccac ggtgacgctc gcgatcgccg cggtcaacga cccgccggtc
     4021 gcggtggccg acgcgacgag cctcgacgag gac
//
LOCUS       DASVOR010001998         3315 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_400281,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001998 DASVOR010000000
VERSION     DASVOR010001998.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400281"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1457)
                     /locus_tag="VF516_37100"
     CDS             complement(<1..1457)
                     /locus_tag="VF516_37100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEX8113425.1"
                     /translation="MSSADLLRETLERRIVVLDGAMGTSVQGLGLDETAWRGERFRDH
                     PVSVKGCIDLLALTRPEAIEQLHLEFLRAGADIVETDTFTATSIALADFGLEGAVRDI
                     NLAAAHIARRAAERAERDDGRPRWVAGSIGPTTKTASLSPDVNDPGARAVTFRQLALA
                     YAEQARALIDGGVDLLITETHIDTLNCKASLFAIAELFDRGARRVPVIASVTIPDRSG
                     RTLSGQTVEAFYNSVAHAELFAISINCALGADDMRPHVAELARIAGVPVACYPNAGLP
                     NEFGGYDDTPEHMAKLLGEFAQGGMLNLVGGCCGTRPAHIAAIRAAVDGVPPRRIPTP
                     PRHMRLAGLEPLTITPSISPSANFIVIGERTNVTGSAAFRRLIKDDRYDEAVAVARQQ
                     VEGGANILDVCMDEGMLDGPSAMRRFLQLIAAEPDIARIPVMVDSSKFAVLEAGLECL
                     QGKSVVNSISLKEGEAEFIRQARIVRRFGAAVVVMA"
     gene            1594..2976
                     /locus_tag="VF516_37105"
     CDS             1594..2976
                     /locus_tag="VF516_37105"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HEX8113426.1"
                     /translation="MPTEMSYATLLDSILDATGMTPMVRLSRIGKTLPCELLAKCEFM
                     NPGGSVKDRIGVRMLLDAERSGRIKPGDTLIEPTSGNTGIGIAMAAAVRGYRVIITMP
                     EKMSQEKQVVLEALGAEIIRTPTEAPWDSPESHIGVARRLKEVIPNSHILDQYSNPSN
                     PAAHEDGTGREIIDQCGGKLDAVVMTAGTGGTITGVARVIKRELPKCKIIGVDPEGSI
                     LAGPGEIRSYKVEGIGYDFIPDVLDRRLVDGWIKSNDRDSFVVARQLIRQEGLLCGGS
                     SGAAVWAAMQVCRDIGRGKRVVVILPDSIRNYLTKFVDDRWMRQQGFAKGDWEVGTVG
                     DCVRALGRREVIALDLNDKLERATDLFKQHGISQMPVLDHGKLVGILTESDLLHHLVS
                     GRANKDTIVAEVMERRVSTVALHASSSELPRIFERGEVAIVVDDQRSVIGILTKMDLI
                     ELLARRPQIS"
BASE COUNT          530 a         1128 c         1185 g          472 t
ORIGIN      
        1 gccatcacga ccaccgcggc gccgaaccgc cggacgatcc gggcctggcg gatgaactcg
       61 gcctcgccct ccttgagcga gatcgagttg accacgctct tgccctgcag gcactcgagc
      121 ccggcctcga gcacggcgaa cttcgatgag tcgaccatca ccgggatccg cgcgatgtcg
      181 ggctcggcgg cgatcagctg gaggaaccgc cgcatcgcgg acgggccgtc gagcatgccc
      241 tcgtccatgc agacgtcgag gatgttggcg ccgccctcga cctgctggcg ggcgaccgcg
      301 accgcctcgt cgtagcggtc gtccttgatc agccggcgga acgcggccga gccggtgacg
      361 ttggtgcgct cgccgatcac gatgaagttg gccgagggtg agatcgacgg tgtgatggtc
      421 aatggctcga gcccggcgag ccgcatgtgg cgcggcgggg tcgggatccg gcgcggcggc
      481 acgccgtcga ccgcggcgcg gatcgcggcg atgtgcgcgg gccgcgtgcc gcagcagccg
      541 ccgaccaggt tgagcatgcc gccctgcgcg aactcgccga gcagcttggc catgtgctcg
      601 ggcgtgtcgt cgtagccgcc gaactcgttg ggcaggccgg cgttggggta gcaggcgacc
      661 ggcacgccgg cgatccgggc gagctcggcg acgtgcggcc gcatgtcgtc ggcgccgagc
      721 gcgcagttga tgctgatcgc gaacagctcg gcgtgggcga ccgagttgta gaacgcctcg
      781 acggtctggc ccgacagcgt gcggcccgag cgatccggga tcgtgaccga cgcgatcacc
      841 ggcacccggc gcgcgccgcg atcgaacagc tcggcgatcg cgaacagcga ggccttgcag
      901 ttgagcgtgt cgatgtgggt ctcggtgatc aggaggtcga cgccgccgtc gatcagcgcg
      961 cgggcctgct cggcgtacgc tagggccagc tggcggaacg tcaccgcgcg ggcgcccgga
     1021 tcgttgacgt cgggcgacag cgacgcggtc ttggtggtcg gaccgatcga gccggcgacc
     1081 cagcgcggcc ggccgtcgtc ccgctcggcg cgctcggcgg cgcggcgcgc gatgtgggca
     1141 gcggcgaggt tgatgtcgcg cacggcgccc tccagcccga agtcggccag cgcgatcgag
     1201 gtcgcggtga acgtgtcggt ctcgacgatg tcggccccgg cgcgcaggaa ctcgaggtgg
     1261 agctgctcga tcgcctcggg ccgggtcagc gcgagcaggt cgatgcagcc cttgaccgac
     1321 accggatgat cgcgaaatcg ctcaccgcgc cacgctgtct catccaggcc gagcccctgc
     1381 acgctcgtgc ccatcgcgcc atcgagcacg acgatgcggc gttcgagcgt ttcgcgtagg
     1441 aggtcggcgg acgacatcgg ccctgattac catcgtttgc ccggggtcgc cacgttgggg
     1501 cggacgaggc gccgtcatcg tggatctcac gctcaccggg ctgcttgcca ggccgccgct
     1561 tggcgctgcg cgtgataggt acagtccacg gcgatgccca ccgagatgtc ctacgcgacg
     1621 ctcctcgact ccattctcga tgcgaccggc atgacgccga tggtgcggct gtcgcggatc
     1681 ggcaagacct tgccgtgcga gctgctcgcc aagtgcgagt tcatgaaccc cggcggctcg
     1741 gtcaaggacc ggatcggcgt gcgcatgctg ctcgacgccg agcgctccgg ccggatcaag
     1801 ccgggcgaca cgctgatcga gccgacctcc ggcaacaccg ggatcggcat cgcgatggcc
     1861 gccgcggtgc gcggctaccg cgtgatcatc acgatgccgg agaagatgtc ccaggagaag
     1921 caggtcgtgc tcgaggcgct gggcgccgag atcatccgga ccccgaccga ggcgccgtgg
     1981 gactcgccgg agagccacat cggcgtcgcg cggcggctca aagaggtcat ccccaactcg
     2041 cacatcctcg accagtactc caacccgtcg aacccggcgg cgcatgagga cggcaccggc
     2101 cgcgagatca tcgaccagtg cggcggcaag ctcgacgcgg tggtgatgac cgccggcacc
     2161 ggcggcacga tcaccggcgt cgcccgcgtg atcaagcgcg agctgccgaa gtgcaagatc
     2221 atcggcgtcg accccgaggg ctcgatcctc gccggaccgg gcgagatccg cagctacaag
     2281 gtcgagggca tcggctacga cttcatcccc gacgtgctcg accgccggct ggtcgacggc
     2341 tggatcaagt ccaacgaccg cgactcgttc gtggtcgccc gccagctgat ccgccaggag
     2401 ggactcctgt gcggcggctc gtcgggcgcc gcggtgtggg cggcgatgca ggtctgccgc
     2461 gacatcgggc gcggcaagcg ggtggtcgtg atcctgcccg actcgatccg caactacctg
     2521 accaagttcg tcgacgaccg ctggatgcgc cagcaggggt tcgccaaggg ggactgggag
     2581 gtcggcacgg tcggcgactg cgtgcgcgcg ctcggccgcc gcgaggtgat cgcgctcgac
     2641 ctcaacgaca agctggagcg cgcgaccgac ctgttcaagc agcacggcat ctcgcagatg
     2701 ccggtgctcg atcacggcaa gctcgtcggc atcctgaccg agagcgacct gctccaccac
     2761 ctggtgagcg ggcgcgccaa caaggacacg atcgtcgccg aggtcatgga gcgccgggtg
     2821 tcgaccgtcg cgctgcacgc cagctcgagc gagctgccgc ggatcttcga gcgcggcgag
     2881 gtcgccatcg tcgtcgacga tcagcgcagc gtgatcggca tcctgaccaa gatggacctg
     2941 atcgagctgc tcgcgcggcg gccccagatc agctagcagc cctggggcgt ggcgccggga
     3001 gagcgcgcgc tcgaccccat ccgctcgcgg acgcccacgg cgacccctca cgtctgatga
     3061 ttcatcacaa gttgcgcatt cgtggccgtt cccgtggtgc tcgacatcga cgtcgacctt
     3121 gaccccagcg ttgcgcttga cggcagcgaa gcgatacgta gacgtctgac cccaccggag
     3181 tgacctggat gaaaggcggt gcaacccagc gagtgaaagt ctcgacctgg caaggaacca
     3241 cagccaggaa gcagggagga cgcgcaggag cagcgagagc acggcgcggt gccctccgac
     3301 aaacccgggg aagcc
//
LOCUS       DASVOR010001999         2241 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_488840,
            whole genome shotgun sequence.
ACCESSION   DASVOR010001999 DASVOR010000000
VERSION     DASVOR010001999.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_488840"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2241
                     /locus_tag="VF516_37110"
     CDS             <1..>2241
                     /locus_tag="VF516_37110"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8113427.1"
                     /translation="RLLLSRVASAPGIPTPIALDELTAGLLDARFEVIERDAGLLLCG
                     ERALLQGARTLLGRPTPCVGRDWELDTLTAILDGGIDEPRAQAVMLIGAAGLGKSRIG
                     AEFVSRAQQRHAELAVWGGRGESLRAGSTLDLLAQALRGAIGIRGSEPLAERQDRIRT
                     RVVEHVPAVGQERVTRFLGELVGAPFPGDGEGGAGLRAARHDPQLMSEQIQTAWLDFL
                     RAETAAHPVLLVLEDLHWSDFGTVRFIGTALRDLCEQPWMVLAMARHELYEVFPRLWA
                     ERQVQEIRLKGLGRRASERLVRQVLGTGAGPQTIERLVQQADGNALYLEELIRAEAEH
                     KDQDAPLPGTVLAMVETRLARLPRDARRVLRVASVFGEVCWEGGVVALLEGAMGARVG
                     ELLATLVEQEVLIERRDGRFPGERELAFRHALLRESAYATLTAEDRRLAHRLAGEWLE
                     QRGEGDPMVLAGHFERGGQSARAAEFYLRAAEQALHVRDPEGVIARAALGLGCAPPQE
                     LRLGLLGIRCEAARRLQRVTISEVEELIREAPRGSLPWARGMLAYQLRALIARRVEDL
                     ADPRAVLQEVTPAPGAAGAMSQVFLTRIFILDSFGLVAQGSALDEPFHALVRARGAQE
                     PLATVWWHVALGVRSSHVHDNPWDAMQHGDAIRPICDALGSQLIFLNMQLVRGMSQWY
                     LGALAFAAQTLEGIPAADTAVGMASSMRRLSLALLYADFGAFDRACAVARQLAESGRA
                     RGNPAEH"
BASE COUNT          268 a          770 c          893 g          310 t
ORIGIN      
        1 cggctgctgc tgtcgcgggt ggcgagcgcg cccgggatcc cgacgccgat cgcgctcgac
       61 gaactgaccg ccggtctgct cgacgcgcgg ttcgaggtga tcgagcgcga tgccgggctg
      121 ctgctgtgcg gcgagcgcgc gctgctccag ggggcgagga cgctgctggg ccgtccgacg
      181 ccgtgcgtcg ggcgcgactg ggagctcgac acgctgaccg cgatcctcga tggcgggatc
      241 gacgagccga gggcgcaagc cgtgatgctg atcggcgcgg cggggctcgg caagtcgcgg
      301 atcggcgccg agttcgtgag ccgcgcccag cagcgccacg ccgagctcgc ggtctggggt
      361 gggcgcggcg agtcgctgcg cgcgggctcg acgctcgacc tgctcgccca ggccctgcgc
      421 ggcgcgatcg ggatccgggg cagcgagccg ctcgccgagc gacaggaccg gatccggacc
      481 cgggtcgtcg agcacgtgcc cgcggtcggg caggagcggg tgacccggtt cctgggggag
      541 ctcgtcggcg cgccgttccc cggcgatggt gagggcggcg ctggcctccg ggcggcgcgg
      601 cacgaccccc agctcatgag cgagcagatc cagacggcgt ggctggattt tctccgcgcc
      661 gagaccgccg cgcacccggt gctgctcgtg ctcgaggatc tgcactggag cgatttcggg
      721 acggtgcggt tcatcggcac ggcgctgcgc gatctctgcg agcagccgtg gatggtgctc
      781 gcgatggcgc ggcacgagct gtacgaggtc ttcccgcggc tgtgggcgga gcgccaggtc
      841 caggagatcc ggctcaaggg cctcgggcgc agggcgagcg aacggctggt ccggcaggtg
      901 ctgggcacgg gcgcgggacc gcagacgatc gagcgcctgg tgcaacaggc cgacggcaac
      961 gcgctctatc tggaggagct gatccgcgcc gaggcggagc acaaggacca ggacgccccg
     1021 ttgcccggga cggtgctggc catggtcgag acccggctcg cgcggctccc gcgcgatgcg
     1081 cggcgggtgc ttcgggtcgc cagcgtgttc ggcgaggtgt gctgggaagg cggtgtggtc
     1141 gcgctgctcg agggggccat gggcgccagg gtcggcgagc tgctcgcgac cctcgtcgag
     1201 caggaggtgc tgatcgagcg ccgcgacggc cggtttcccg gcgagcgcga gctcgcgttc
     1261 cgccacgcgc tgctgcgcga gagcgcgtac gcgaccctga ccgccgagga tcggcgcctc
     1321 gcgcaccgcc tcgccggcga gtggctcgag cagcgcggcg aaggcgatcc catggtcctg
     1381 gccgggcact tcgagcgcgg cgggcagtcc gcgcgcgcgg ccgagttcta cctgcgcgcg
     1441 gcggagcagg cgcttcacgt ccgcgacccc gagggcgtga tcgcgcgcgc ggccctcggc
     1501 ctgggctgcg cgcctccgca ggagctgcgg ctcggtctgc tcggcatccg ctgcgaggcg
     1561 gcccggcgcc tgcagcgggt cacgatcagc gaggtcgagg agctgatccg cgaggcaccg
     1621 cgcggctccc tgccctgggc acgcgggatg ctcgcgtacc agctccgcgc gctgatcgcg
     1681 cgccgggtcg aggatctcgc ggacccgcgg gcggtgctcc aggaggtcac tccggcgccc
     1741 ggggccgcgg gcgcgatgtc ccaggtgttc ctcacccgga tcttcatcct cgatagtttc
     1801 gggctcgtcg cgcagggcag cgcgctggac gagccgtttc acgcgctggt ccgcgcgcgc
     1861 ggagcgcagg agccgctcgc gaccgtgtgg tggcacgtcg ccctcggcgt ccggagctct
     1921 catgtgcacg acaacccctg ggacgcgatg cagcatggcg acgcgatccg gccgatctgc
     1981 gacgccctcg gcagccagct gatcttcctg aacatgcagc tggtgcgagg gatgagccag
     2041 tggtacctcg gggcgctcgc gttcgcggcg cagacgctgg agggcatccc ggcggcggat
     2101 acggcggtcg gcatggcgag ctcgatgcgc cggctgtcgc tggcgttgct gtacgccgac
     2161 ttcggtgcgt tcgaccgcgc ctgcgcagtg gcccgccagc tcgccgagtc aggccgcgcg
     2221 cgcgggaacc cggcggagca c
//
LOCUS       DASVOR010002000         1582 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_22305,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002000 DASVOR010000000
VERSION     DASVOR010002000.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22305"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..338)
                     /locus_tag="VF516_37115"
     CDS             complement(48..338)
                     /locus_tag="VF516_37115"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8113428.1"
                     /translation="MHDDLTDSQRHLRDFIVTNFYIPDGQPLDEVTSFLDAGIIDSTG
                     VLELVAHVESKYGITIADHELIPSNFDSIGALARFIQRKRGRDGGGHDRLAR"
     gene            complement(343..>1582)
                     /locus_tag="VF516_37120"
     CDS             complement(343..>1582)
                     /locus_tag="VF516_37120"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8113429.1"
                     /translation="AEPARSSPYLATVMITGTTDDARVAAACERLAALLPALPRLTTM
                     ADAIAEGPHDAPPPRRCIDIDLAAIIYTSGSTGEPKGVMLSHRNMVAATASIASYLEI
                     TRDEVILCVLPLAFDYGLYQMILACAAGARVVLERSFAFPAEVMRTVAGERVTGFPGV
                     PTIFATLLGMDSLGAYDLSAIRYFTSTAAALPVKHIEQIRRRFPAAKLFSMYGLTECK
                     RCTYLPPEDLARKPDSVGIAIPNTELWLVDDHGHRLGPHQVGELVIRGATVMQGYWDK
                     PEATARKLRPGPLPGERVLYTGDLCRLDDDGYLYFVGRTDEVIKSRGEKVPPREVEAT
                     LLGISGVREAAVIGVPDDVLGNAIKAFVVPEPGVVLDVSALQRECRARLESFKVPHQI
                     ELRAALPRSSNGKIAKSELG"
BASE COUNT          229 a          548 c          566 g          239 t
ORIGIN      
        1 cgatccgcgc gacctcggcc tcggcctcgc ccgcggcgaa cccgacgtca acgcgccagc
       61 cggtcatggc cacctccgtc gcggcctcgc ttgcgctgga tgaagcgggc gagcgctccg
      121 atcgaatcga agttggaggg aatcagctcg tgatccgcga tcgtgatgcc gtacttggac
      181 tcgacgtgtg ccaccagctc gagcacgccg gtcgaatcga tgatgccggc gtcgaggaac
      241 gacgtcacct cgtcgagcgg ttgcccgtcc gggatgtaga agttggtgac gatgaagtcg
      301 cgcaggtggc gctgcgaatc ggtcaggtcg tcgtgcatgt cgtcacccca gctcgctctt
      361 cgcgatcttg ccgttgctgc tccgcggcag cgccgcgcgc agctcgatct ggtgcgggac
      421 cttgaagctc tccagccgtg cgcggcactc gcgctgcagc gccgacacgt cgagcaccac
      481 gccgggctcg ggcacgacga acgccttgat cgcgttgccc agcacgtcgt cgggcacgcc
      541 gatcaccgcg gcctcgcgca cgccggagat cccgagcagc gtcgcctcga cctccctggg
      601 cgggaccttc tcgccgcgcg acttgatcac ctcgtcggtc cggcccacga agtacaggta
      661 gccatcatcg tcgagccggc acagatcgcc ggtgtagagc acgcgctcgc cgggcaaggg
      721 acccggccgc agcttgcgcg cggtggcctc gggcttgtcc cagtagccct gcatcacggt
      781 cgccccgcgg atcaccagct cgccgacctg gtgggggccg agccggtggc cgtggtcgtc
      841 gaccagccac agctcggtgt tggggatcgc gatgccgacg ctgtcgggct tgcgcgcgag
      901 gtcctcgggc gggaggtacg tgcagcgctt gcactcggtc agcccgtaca tcgagaacag
      961 cttggcggcc gggaaccggc ggcggatctg ctcgatgtgc ttgaccggca gcgccgcggc
     1021 ggtgctggtg aagtagcgga tcgccgacag gtcgtacgcc cccagcgagt ccatgccgag
     1081 cagcgtcgcg aagatcgtcg ggacgcccgg gaagccggtg acgcgctcgc cggcgacggt
     1141 ccgcatcacc tcggcgggga acgcgaacga ccgctccagc acgacgcgcg cgccggccgc
     1201 gcatgccagg atcatctggt acagcccgta gtcgaacgcg agcggcagca cgcacaggat
     1261 cacctcgtcg cgcgtgatct cgaggtagct cgcgatcgac gcggtcgcgg cgaccatgtt
     1321 gcggtgcgac agcatcacgc ccttgggctc gccggtcgat cccgaggtgt agatgatcgc
     1381 ggcgagatcg atgtcgatgc accgccgggg cggcggggcg tcgtgggggc cctccgcgat
     1441 cgcgtcggcc atcgtggtca gcctgggcag cgcggggagc agcgcggcga gccgctcgca
     1501 cgcggccgcg acccgcgcat cgtcggtggt gccggtgatc atcacggtcg cgaggtacgg
     1561 cgagctccgc gccggctcgg cg
//
LOCUS       DASVOR010002001         1860 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_79700,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002001 DASVOR010000000
VERSION     DASVOR010002001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1860
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79700"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1032
                     /locus_tag="VF516_37125"
     CDS             <1..1032
                     /locus_tag="VF516_37125"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8113430.1"
                     /translation="GVHAAASDCRTQICVHNENTDLTVFDGATYLVHRTAASQILGPN
                     SSLRVLRSSDHGGAWQLLAVIPAPDGRDLRDPSFYQVGGKLAIKALTHRQVISLRDSD
                     VDTIAVASVSPDGGRTWGPLAPIGPERWSFWRIRDDARGVHYTAAYEDGDRSVQLFSS
                     SDGTHWTDVSRIYGRAEDTPLETELVFVPSGKLLALVRLDGNNAELFGNAGRLRTRVC
                     WAAPPFATWDCSRELVGVRLDGPVAFFDRGRLFVVARKHLLEPEARKRTALYELTGDL
                     DGGDLAIVERGELPSAGDTAYAGVAPIERDRFLVTWYSSRLSADEPWLVGALGPTDIW
                     QATLDLSQL"
     gene            complement(1038..>1860)
                     /locus_tag="VF516_37130"
     CDS             complement(1038..>1860)
                     /locus_tag="VF516_37130"
                     /inference="COORDINATES: protein motif:HMM:NF012859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ricin-type beta-trefoil lectin domain protein"
                     /protein_id="HEX8113431.1"
                     /translation="ALGTNLALGRSVTASAACASTEGAANAVDGVLANNSKWCSLTSG
                     ASLQVDLGSAQTVGSFVVKHAGLGGETTGWNTGAFAIDTSTDGATFTTVVNVSGYRSS
                     RSFHPVTPRSARFVRLRSLTPTNNGNTATRIYELEVYASPGTTATGAIPSGIAGKCIT
                     DAGGATANGTPITLSTCNGAAAQTWQSPGDGTLRIAGKCMDVTGSGTANGTRIQLFDC
                     NGSGAQQWYWNSASKSLVNPASQRCLDDPSSSTTDGTQLQLFDCNLTAAQQWTLP"
BASE COUNT          238 a          671 c          675 g          276 t
ORIGIN      
        1 ggcgtccacg ccgcggcgag cgactgccgg acccagatct gcgtccacaa cgagaacacc
       61 gacctgacgg tgttcgacgg tgcgacctac ctggtgcatc gcaccgcggc cagccagatc
      121 ctcgggccga acagctcgct gcgcgtgctg cgctccagcg accacggcgg cgcctggcag
      181 ctgctcgcgg tgatccccgc cccggacggc cgcgacctgc gcgatccgag cttctaccag
      241 gtcggcggca agctcgcgat caaggcgctg acccaccggc aggtcatctc gctgcgcgac
      301 agcgacgtcg acaccatcgc ggtcgccagc gtgtcgcccg acggcggccg cacgtggggc
      361 ccgctcgcgc cgatcggccc cgagcgctgg agcttctggc ggatccgcga cgacgcccgc
      421 ggcgtgcact acaccgcggc ctacgaggac ggcgaccgga gcgtccagct gttctcgtcg
      481 agcgacggca cgcactggac cgacgtgtcg cggatctacg gccgcgccga ggacacgccg
      541 ctcgagaccg agctggtgtt cgtgccgtcg ggcaagctgc tcgcgctggt ccggctcgac
      601 ggcaacaacg ccgagctgtt cggcaacgcc gggcggctgc gcaccagggt ctgctgggcg
      661 gcgccgccgt tcgcgacctg ggactgctcg cgcgagctcg tcggggtccg gctcgacgga
      721 ccggtcgcgt tcttcgaccg tggtcggctg ttcgtggtcg cccgcaagca cctgctcgag
      781 cccgaggcca gaaagcgcac cgcgctctac gagctcaccg gcgacctcga cggcggcgac
      841 ctggcgatcg tcgagcgcgg cgagctgccg tcggccggcg acaccgcgta cgccggggtc
      901 gcgccgatcg agcgcgaccg cttcctggtg acctggtact cgagccggct gtcggccgac
      961 gagccgtggc tggtcggcgc gctcggcccg accgacatct ggcaggccac gctcgatctg
     1021 tcgcagctat aggctcccta cggcagcgtc cactgctgcg cggcggtcag gttgcagtcg
     1081 aacagctgca gctgggtgcc gtcggtggtc gagctcgacg gatcgtcgag gcagcgctgg
     1141 gacgcggggt tgaccagcga cttgctcgcc gagttccagt accactgctg ggcgccgctg
     1201 ccgttgcagt cgaacagctg gatccgggtg ccgttggcgg tgccgctccc cgtgacgtcc
     1261 atgcacttgc cggcgatccg cagcgtgccg tcgcccggcg actgccaggt ctgcgccgcc
     1321 gcgccgttgc aggtcgacag cgtgatcggc gtgccgttgg cggtggcgcc gcccgcgtcg
     1381 gtgatgcact tgccggcgat ccccgagggg atcgcgccgg tcgcggtcgt gccgggcgac
     1441 gcgtagacct cgagctcgta gatccgggtc gcggtgttgc cgttgttggt cggcgtcagg
     1501 ctgcgcagcc ggacgaaccg cgccgagcgc ggcgtgaccg gatggaacga gcggctcgac
     1561 cggtagccgc tgacgttgac caccgtggtg aacgtcgcgc cgtcggtgct ggtgtcgatc
     1621 gcgaacgcgc cggtgttcca cccggtggtc tcgccgccca ggccggcgtg cttgaccacg
     1681 aagctgccga cggtctgggc cgagccgaga tcgacctgca ggctggcgcc cgaggtcagc
     1741 gagcaccact tgctgttgtt ggccagcacg ccgtcgaccg cgttggccgc gccctcggtg
     1801 ctcgcgcacg ccgccgacgc ggtgaccgag cggccgagcg ccaggttcgt gcccagcgcc
//
LOCUS       DASVOR010002002         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_342168,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002002 DASVOR010000000
VERSION     DASVOR010002002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342168"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="VF516_37135"
     CDS             <1..1266
                     /locus_tag="VF516_37135"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEX8113432.1"
                     /translation="PSAVNTIVSKYICLRYSLGAVDRAAPGAPALYMVGEPLPGFRSW
                     SEALAAQPETPIADEIAGQDMLYSSGTTGRPKGIKRPEEHNPIDQPNPMLRFLCADLC
                     GMAADSVYLSPAPLYHAAPLRFNMMSAVLGGTSVIMEAFDAEEFLQLVERHRVTQSQL
                     VPTMFVRMLKLPEEVRRRYDLSSLRGAIHAAAPCPVDVKQQMIDWWGPILIEYYAGSE
                     GNGATVCTSEQWLAHRGSVGRAVVGTVKIVDEYDRELPPGGIGAVYFAGGPAFQYHND
                     DERTRRAHNHRGWSTLGDVGYLDGDGYLYLTDRKAFMIISGGVNIYPQETEDVLITHP
                     EVVDVAVLGVPDEEMGEQVKAVVQLHDMSRAGPAMAAELIGFCRQRLSPVKCPRSVDF
                     EAELPRTPTGKLMKRLLRDLYWRPAVAPR"
     gene            complement(1268..>1877)
                     /locus_tag="VF516_37140"
     CDS             complement(1268..>1877)
                     /locus_tag="VF516_37140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113433.1"
                     /translation="AAAGSSEHGRSARRSPSTRIQAPFVLAEIRALRSSIFKRSREVQ
                     SALAIAHKVEHSYDLHNAELFLPLDDTWCAILEPFELTVVTGTLRRIGYEIFPQYVSI
                     LGIPAPEVNTAMDLHGPTDLIRVICNAYNKCVVGDEAGTLSPGIDGTRTTVTDTTIMP
                     CQLQMGVFLGAGQLTGLFRDSAVTETRCRSRGDSACVYEFAF"
BASE COUNT          286 a          636 c          671 g          284 t
ORIGIN      
        1 ccctcggccg tgaacaccat cgtgtcgaag tacatctgcc tcaggtactc gctcggcgcc
       61 gtcgatcgcg ccgcgcccgg cgcgccggcg ctgtacatgg tcggcgagcc gctgccgggg
      121 ttccggtcgt ggagcgaggc gctcgcagct cagcccgaga cgccgatcgc cgacgagatc
      181 gccggccagg acatgctgta ctcgtcgggg acgaccggcc ggcccaaggg catcaagcgc
      241 ccggaggagc acaatccgat cgatcagccc aatccgatgc tgcgcttcct gtgcgccgac
      301 ctgtgcggga tggcggcgga cagcgtctac ctgtcccccg cgccgcttta ccacgcggcc
      361 ccgctgcgct tcaacatgat gtcggcggtg ctcggcggca cgtcggtgat catggaggcg
      421 ttcgacgccg aggagttcct ccagctggtc gagcgccatc gcgtcaccca gtcgcagctg
      481 gtgccgacga tgttcgtgcg catgctgaag ctgcccgagg aggtgcggcg gcgctacgat
      541 ctgagctcgc tgcgcggcgc gatccacgcc gcggcgccat gcccggtcga cgtcaagcag
      601 cagatgatcg actggtgggg cccgatcctg atcgagtact acgcgggctc ggagggcaac
      661 ggcgccacgg tgtgcacctc cgagcagtgg ctcgcccacc gcggcagcgt cgggcgcgcg
      721 gtggtcggca cggtcaagat cgtcgacgag tacgaccgcg agctgccgcc cggcgggatc
      781 ggcgcggtgt acttcgccgg cggaccggcg ttccagtacc acaacgacga cgagaggacc
      841 cggcgcgcac acaatcaccg gggctggtcg acgctcggcg acgtcggcta cctcgacggc
      901 gacggctacc tgtacctcac cgatcgcaag gcgttcatga tcatctcggg tggcgtgaac
      961 atctatccgc aggagaccga ggacgtgctg atcacgcacc ccgaggtcgt cgatgtcgcg
     1021 gtgctcggcg tgcccgacga ggagatgggc gagcaggtca aggcggtggt ccagctgcac
     1081 gacatgagcc gcgccgggcc ggcgatggcg gccgagctga tcgggttctg ccgccagcgg
     1141 ctgtcgccgg tcaagtgccc gcgcagcgtc gacttcgagg ccgagctgcc gcgcacgccg
     1201 accggcaagc tgatgaagcg cctgctgcgc gatctctact ggcggccggc ggtggcgccg
     1261 cgctgagcta gaacgcgaac tcgtacacgc aggcgctgtc gccgcggctg cggcagcgcg
     1321 tctcggtcac cgcgctgtcg cggaacagcc cggtcagctg cccggcgccg aggaacacgc
     1381 ccatctgcag ctggcacggc atgatcgtcg tgtcggtgac ggtcgtgcgg gtaccgtcga
     1441 tcccggggct gagcgtgccg gcctcgtcac cgaccacgca cttgttgtag gcgttgcaga
     1501 tcacccggat cagatcggtc gggccgtgca ggtccatcgc cgtgttcacc tcgggcgccg
     1561 ggatgccgag gatcgagacg tactgcggaa agatctcgta gccgatccgg cgcagcgtgc
     1621 cggtcaccac cgtgagctcg aacggctcga ggatcgcaca ccaggtgtca tcgagcggaa
     1681 ggaacagctc ggcgttgtgg aggtcgtacg agtgctcgac cttgtgagcg atcgccagcg
     1741 cgctctggac ctcgcggctg cgcttgaaga tcgatgaccg cagcgcgcgg atctcggcca
     1801 gcacgaacgg ggcctggatc cgggtgctcg ggctccggcg cgcggagcgg ccgtgctcgc
     1861 tcgacccggc ggcggcc
//
LOCUS       DASVOR010002003         4189 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_255515,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002003 DASVOR010000000
VERSION     DASVOR010002003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255515"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="VF516_37145"
     CDS             complement(<1..570)
                     /locus_tag="VF516_37145"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8113434.1"
                     /translation="MSTHDVVILGGGVSGLSFAFHAARAGRSVHVIEQDARLGGCVDT
                     RTTGDGFWIELGAHTCYNSYGAFIELLEGVDLMGELQPRGKPVLRFLDHDAILPGKNL
                     GALLRGMSLLQLARSMPRLIGASPEGKTVRALYGRIAGDDTYERVLGPMLSAVPSQRA
                     DDFPADMLFKKRPRHKDVLRSFTLRGGLGA"
     gene            671..3862
                     /locus_tag="VF516_37150"
     CDS             671..3862
                     /locus_tag="VF516_37150"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX8113435.1"
                     /translation="MSAERCVHTLVVEQAARTADAIALSTEAGCLSYRELDEASNRIA
                     HHLRALGVGREVLVALCLERSLEMVPVILGVLKAGGAYVPLDPTDPPGRLRALLADCK
                     PRVLLTHERLRPTFETLSLAVRAVDGLGIFADDSAAPPSSAAVPDNLAYVIYTSGSTG
                     TPKAVMCTHRGLVDTLEWGRSTYALAATDRIPLKTPYTFDPSLLELFWPLMVGGTIVV
                     ARPDGHKDPIYLVDLIRTYEITIAQFVPSALRAFLEVEGVESLRSLRLLIVGGEALPL
                     DLHDRLLSRLPVELVNHYGPTEASIDAAVWRCRRDPGRTVVPIGGPIADTRLDVVDSE
                     LRPVPTGVAGELLIGGTRLARGYLNRPSLTAERFVPDPLGQPGERRYRTGDLVRQDVD
                     GNLEFLGRIDHQIKIRGFRVELEEIEVILGQHDAVSESAVVPREDAPGERHLVAYIVP
                     RGTEAPSVPDLRDHLRARLPEHMVPEAFVFVDALPRSTAEKVDRAALAARSVARSSDP
                     SDRSVPPTRRRAALLPQVPYVAPSGAVEQAIAAVWQDVLAVDSIGAQDRFLDLGGESL
                     RATRATLRLRELLGRDVPIVVMLQNASVAELAVHVGALPARAAGPQAGARPRGAAVAR
                     RPTVRSGRFPATRAQSRFWMEAQLGFDNAMPELLELEGPLDLRALRLSLDMLVKRHAA
                     LRTTFDVAGSDVIQTIGLPVPASIEMLDLCAEPPERRDERAMELVADRSRVPFDLQRG
                     PMFRTHLMKLAPDRHLMLWNLHHILGDHVSIFLLYEELSALYRGFVTGSPATLPPIEV
                     DAVDVARLERFEVPGEETIAYWRSALDGQAPPLDLPVARPRGERKTLRGGVCPIYMSQ
                     LPTDGLRALAITEGVTFHMAVLSALFALLHRYSEREDLVIGVPATTRERIEYERTVGN
                     FVNLLPVRLQFTGTLSFRQLLARVRATTLEAYRHKHMPFDRIIAEVAPRRSAAHNPLY
                     QVLLELARDGEVSLHGLRVRRPWPPHPGRQLLDLSFVLSEEDHQVWGELAYNADLFDA
                     DAAAGIAEHFVVLIEHAVANPDLMIAELAMTRD"
     gene            complement(3894..>4189)
                     /locus_tag="VF516_37155"
     CDS             complement(3894..>4189)
                     /locus_tag="VF516_37155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113436.1"
                     /translation="VIFNFLDLREAPLPELPGAEVSLATRPSDPADVIMNDLAVFIAD
                     GEAAIAAEVVCNADLFTDEGLRRVATDYLDLIAWVRRNPDRTVQDARARGSRP"
BASE COUNT          631 a         1415 c         1420 g          723 t
ORIGIN      
        1 cgcgccgagg ccgccgcgca gggtgaagct gcgcagcacg tccttgtggc gcgggcgctt
       61 cttgaacagc atgtccgcgg ggaagtcgtc ggcgcgctgc gacggcacgg cagacagcat
      121 cgggcccagc acgcgctcgt aggtgtcatc gccggcgatc cggccgtaga gcgcgcgcac
      181 ggtcttgcct tccggcgacg cgccgatcag ccggggcatc gagcgcgcga gctgcagaag
      241 gctcatgccg cggagcaggg cgccgaggtt cttgccgggc aggatcgcgt cgtggtcgag
      301 gaagcgcagc accggcttgc cgcgcggctg gagctcaccc atcaggtcga cgccctcgag
      361 cagctcgatg aacgcgccgt acgaattgta gcaggtgtgc gcgccgagct cgatccagaa
      421 cccgtcgccg gtggtccggg tgtcgacgca gccgccgagc cgcgcgtcct gctcgatgac
      481 gtgcacgctg cggccggcgc gcgcggcgtg gaacgcgaag ctgaggccgg acacgcctcc
      541 gccgaggatg acgacgtcgt gggtggacac ggcgccatgg tacacggggc tgcccgcggc
      601 ttcgcgtgtc aaccctggcc atcaaccaac gcgatcatgc ttagatggcc gtgcgacgat
      661 ggttcgccac atgagcgccg agcgttgtgt tcacacgttg gtagtcgagc aggccgcgcg
      721 aacagccgat gcgatcgcgt tgagtacgga agccggttgc ctttcgtacc gggagctaga
      781 cgaagcgtcg aaccggatcg ctcaccactt gcgggcactc ggcgtcgggc gcgaggtgct
      841 cgtcgcgctc tgtctcgagc gatcgctcga gatggttccc gtgatcctcg gtgtgctgaa
      901 ggccggcggg gcatacgtcc cgctcgaccc gaccgatcct ccagggcgac tccgcgcctt
      961 gctcgcggat tgtaagcccc gcgtcctcct cacgcacgag cgattgcggc ccacgttcga
     1021 gaccctctcg ctcgccgtgc gcgcggtgga cgggctgggc atattcgcgg acgacagcgc
     1081 ggcgcccccg agctcggctg cggtgccgga caacctggcg tacgtgatct acacgtccgg
     1141 atcgacgggc actcccaagg cggtgatgtg cacccaccgg ggcctcgtcg ataccctcga
     1201 gtgggggcgc agcacgtatg cactcgcagc aaccgatcgc ataccgctga agaccccgta
     1261 caccttcgac ccatcgctgc tggagctgtt ctggccgctc atggtggggg ggacgatcgt
     1321 ggtggctcgg ccggacgggc acaaggatcc gatctatctc gtcgacctga tccggacgta
     1381 cgagatcacg atcgcacagt tcgtgccttc ggcgctgcgc gcgtttctcg aggtcgaggg
     1441 cgtcgagtcg ctgcgctcgt tgcgactctt gatcgtcggc ggcgaggcgt tgccgctcga
     1501 tctccacgat cgcttgctct cgcgacttcc cgtcgagctt gtcaaccatt atggtccgac
     1561 ggaggcgtcg atcgacgccg ccgtctggcg gtgccggcgc gatcccggcc gcacggtggt
     1621 tccgatcggc ggaccgatcg ccgacacgcg cctcgatgtc gtcgattccg agcttcggcc
     1681 cgttccgacc ggcgttgccg gtgagctcct gattggcgga acccggctgg ctcgcggcta
     1741 cctgaatcgt ccgagcctca ccgcggagcg attcgtgccg gatccgctgg ggcagccagg
     1801 cgagcggcgc taccgaacgg gtgacctggt ccgccaggac gtggacggga acctggaatt
     1861 cctgggacgc atcgatcatc agatcaagat ccgaggtttc cgcgtcgagc ttgaggagat
     1921 cgaggtcatc ctcgggcagc acgacgctgt ctccgagtcc gctgtcgtgc ctcgcgagga
     1981 tgctccaggc gaacggcatc tcgtcgcata catcgtcccg cgagggacgg aggcgccctc
     2041 cgttccggat ctgcgggacc acctgcgtgc acggctaccc gaacacatgg ttcccgaagc
     2101 gttcgtgttc gtcgacgccc tgccgcgctc gacggccgaa aaggtcgacc gcgccgcgct
     2161 cgcggctcga tcggtcgcgc gatcctcgga tccttcggat cgctccgttc caccgacccg
     2221 acggcgcgcc gcgctccttc cgcaagtccc ctacgtggcg ccgagcggcg cggtagaaca
     2281 ggcgatcgcg gccgtctggc aggacgtgct cgcggtcgat tccatcggcg cgcaggaccg
     2341 gtttctcgac ctcggcggcg aatcgctgcg tgcgacacgt gccacgctgc gcttgcgcga
     2401 gctgctcggc agggatgtcc cgattgtcgt gatgttgcag aacgcgagcg tcgcagagct
     2461 cgccgttcat gtcggggccc tgcccgcgcg tgctgcgggc ccgcaggcag gggcgcggcc
     2521 gcgtggtgcc gccgttgctc gtcggcccac cgtccgatcc ggcaggttcc cggcgacccg
     2581 tgctcagagc cggttctgga tggaggctca gctcggcttc gacaatgcca tgcccgagct
     2641 cctggagctg gaggggcccc tcgatctgcg cgcacttcgg ctgagcctgg acatgctggt
     2701 gaagcgacat gcggcgctgc gcacgacgtt cgacgtcgcc ggcagtgacg tcatccagac
     2761 gatcggcctc ccggtgccgg cgtcgatcga gatgctcgat ctctgtgccg aaccgcccga
     2821 gcgtcgcgat gagcgcgcca tggaactggt cgccgatcgc tccagggtgc cgttcgatct
     2881 gcaacgggga ccgatgtttc gcacgcactt gatgaagctc gctcccgatc gtcacttgat
     2941 gttgtggaac ctgcaccaca tcctcgggga ccacgtgtcc atcttcctgc tttacgagga
     3001 gctcagcgcg ctctaccgcg gcttcgtcac cggctcgcct gccacgctgc cgccgatcga
     3061 ggtcgacgcg gtcgacgtcg ctcggctgga gcggttcgaa gtgccgggcg aggagacgat
     3121 cgcgtactgg cgcagtgcgc tcgatggaca ggctccgccc cttgacctgc cggtggcacg
     3181 gccacgaggc gaacggaaga cactgcgcgg tggggtctgt ccgatctaca tgtcgcagct
     3241 gccgaccgac ggcctccgag cgctggcgat cacggaagga gttaccttcc acatggccgt
     3301 gctgagcgcg ctgttcgctc tgctccatcg atacagcgag cgcgaggatc tcgtcatcgg
     3361 cgttccagcg accacgcgcg aacgtattga atacgagcgc accgtcggca acttcgtcaa
     3421 tctgctgccg gtgcggctcc agttcaccgg tactctatcg tttcgccagc tgctcgcacg
     3481 cgtgcgcgcg accacactgg aggcgtaccg ccacaagcac atgccattcg atcggatcat
     3541 cgcggaggtt gcaccgcgcc gtagtgcggc acacaacccg ctgtaccagg tactgctcga
     3601 gctcgcacgg gatggcgagg tatcgctgca cgggctacgc gtgcgtcggc cctggccccc
     3661 ccatccgggc cgccagctgc tcgacctctc cttcgtcctg tcggaggagg accatcaggt
     3721 gtggggcgag ctggcgtaca acgctgatct gttcgacgcc gatgcggccg cggggatcgc
     3781 ggagcacttc gtcgtcctga tcgagcacgc cgtcgcgaac ccagacctga tgattgccga
     3841 gctcgcgatg acccgggact gacccggact gacccggact gacgatcaac acgtcatggc
     3901 cgggagccgc gtgcgcgggc gtcctgaacg gttcgatccg ggttgcgcct cacccatgcg
     3961 atcaggtcga ggtagtccgt ggccacgcgg cgcaatcctt cgtccgtgaa caggtcggcg
     4021 ttgcaaacga cctcggcggc gatggcggcc tctccgtcgg cgatgaacac cgcgaggtcg
     4081 ttcatgatca cgtcggcggg atcgcttggc cgggtcgcga ggctcacctc cgcaccgggc
     4141 aactcgggca gcggcgcttc gcgcaggtcg agaaagttga agatgacgc
//
LOCUS       DASVOR010002004         5682 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_167093,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002004 DASVOR010000000
VERSION     DASVOR010002004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5682
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167093"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VF516_37160"
     CDS             <1..368
                     /locus_tag="VF516_37160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113437.1"
                     /translation="FGVSRTWAAYTEFVDVGTGPQEFSLFANGTILNIPVGWVPTAAL
                     NYGGIAALTQVSLGLDKFGVWSYDEQLVFLLNAVPLVGIALGFGFGEGGYGPDYKGNT
                     GWYDSFKGFLHDRLSMESI"
     gene            complement(325..621)
                     /locus_tag="VF516_37165"
     CDS             complement(325..621)
                     /locus_tag="VF516_37165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113438.1"
                     /translation="MSPKRTFWQWLVGREPAPPAIPPPAAEPQHLGSADEAFLAKLVA
                     DLADGKRRGEIADAEVLRALDGLWTSGHERLAIEWISLASIRCSPCSGGRAGTL"
     gene            1109..2179
                     /locus_tag="VF516_37170"
     CDS             1109..2179
                     /locus_tag="VF516_37170"
                     /inference="COORDINATES: protein motif:HMM:NF014395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113439.1"
                     /translation="MTLDEIPPVRQPLSSRRTFTWYRPAGRSATEYEDLTCGQQSDPA
                     HYAYQGWPIRFDDGRDPVYAGSTALRSTDWFAFRDPSHTTNRAYVASTNEAERSLSRS
                     IAGAVKAGHFAFAEPGWVRVGLAQHFLTYPFVNYGLFLAFCYAEREALSDTVTFAIVF
                     TAADKLRHMQDAVHYSYELEAAFGTSDAEAPARWKADPVWQGARAAVEHIIASNDWME
                     IVIAANLSFDRVFGDLAKVEYFSRFAAANGDGITPMMIASAAADDHRATAWTRALVHH
                     LVSDAAHGAANRKQISEWIDKWDARSKQAIDGFAPCFRAAPRNPVSFADAAASVWRRR
                     NQLLAELELTPSRISVEGGGHV"
     gene            2172..2501
                     /locus_tag="VF516_37175"
     CDS             2172..2501
                     /locus_tag="VF516_37175"
                     /inference="COORDINATES: protein motif:HMM:NF014461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmoB/DmpM family protein"
                     /protein_id="HEX8113440.1"
                     /translation="MSEIATSPLEPRARDTVGISLMGSEETTAALELVRAEMPDARIS
                     DRGCFYKIEREVLLELDMAKLGEILGRPITVHQFLVNMSSYYGRIVINDGLLQIHSDI
                     LPARFRD"
     gene            2517..4022
                     /locus_tag="VF516_37180"
     CDS             2517..4022
                     /locus_tag="VF516_37180"
                     /inference="COORDINATES: protein motif:HMM:NF014395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113441.1"
                     /translation="MTTPKLEQVHEKSKRYDWGFDYAIPNPKYRTRYLIPPRGKDPFR
                     VLIRDYMAMENEKDNRVFGALDADVRYKNAERAEPRWVEAMKFALPAFTDAEYQAVKG
                     AGFLITSVQNQELRQGYAAQMLDEIRHTQLETALRRYYLKNYHDPAGFDIGQRALGNH
                     PIGTLARASFQPFNTGDPIEVAMCLNVVLETAYTNPLVVALPQAAVANGDHALATTFL
                     SIQSDESRHMANGYGTLMCCVAQPDNIPFLNESLERHFWHQHMSMDTLVGVASEYYTV
                     NRPWRYRDVWEEWVVDDFIGSYMTRLREFGLRPPQRLPEVARFVDDMHHSAALALAAI
                     WPLNFWRIDPLTERDYDWFENAYPGWHARYGGFWDMYRRMSDPASGRIPMQVLPSLPP
                     FCQVCHAPCVMPRFDQPETRVFEHGGKRYAVCSEGCEWIFRLNPTIYTGCANWWEPWH
                     GMNLADVIEALGYVRPDGRTLMAQPHLGASRMWTLDDIRRLDYVIKDPLRS"
     gene            4024..5073
                     /locus_tag="VF516_37185"
     CDS             4024..5073
                     /locus_tag="VF516_37185"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEX8113442.1"
                     /translation="MASPPPRRIRIEPFGEALEIRAGESVLAAILRQGRFVRYGCKHG
                     GCGTCRAQRVSGDCELSSRTSFSLSDADRASGVVLLCSTYVTSGEAVFDVSRTMELSE
                     EEFRSTGRVAEYIAEVAAIESPTPDIRRVRFQLVDPPAMRFTAGQYVEVAVPGTDSEW
                     RSYSMSSDPADRGALEIVVKRIPGGRFSSALDGRLRSGDRLRLRGPMGQFAVRWSHRP
                     MVMVAGGSGIGPIRAMLWELVRTGNARPVHLFYGARRRSDLFLLEELRAVEHDQRWFR
                     LTPTLSEPAPGAAWDGEVGLVTDVLARELPHTRGTEAYLCGSPGMIDAAIRVLIAAGC
                     KEKQIYFDRFVPSGP"
     gene            complement(5116..>5682)
                     /locus_tag="VF516_37190"
     CDS             complement(5116..>5682)
                     /locus_tag="VF516_37190"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8113443.1"
                     /translation="LDTSLLTGGRVELRRRGKPAIVACAAPKIVLGRDPLCDLALRAG
                     GVSRQHAEIERSDAGFALRDLDSRNGTSLAGMPLAGRLPLGVAGELGLGEECPIDYEV
                     TGPILVLRVRGGLDRGAALIAGAEDQRLDLTPLGLGLDLIFHRGRPLLGRGSCTSVTF
                     NDEPLGDVRVQLIRKDRILADGDEIEVA"
BASE COUNT          885 a         1906 c         1961 g          930 t
ORIGIN      
        1 acttcggcgt cagccggacc tgggccgcgt acacggagtt cgtcgacgtc ggcaccgggc
       61 cgcaggagtt ctccttgttc gccaacggga cgatcctgaa catcccggtc gggtgggtgc
      121 ccacggcggc gctcaactac ggcggcatcg cggcgctgac ccaggtctcg ctgggcctgg
      181 acaagttcgg ggtctggtcc tacgacgagc agctcgtctt cctgctcaac gccgtccccc
      241 tggtcggcat cgcgctgggt ttcggcttcg gcgagggcgg gtacggtccc gattacaagg
      301 ggaacaccgg ctggtacgat tccttcaaag ggttcctgca cgaccgcctg agcatggaga
      361 gcatctgatg gatgccaagc ttatccactc gatcgcgagc cgctcgtgcc ccgacgtcca
      421 cagcccgtcg agcgcgcgca gcacctcggc gtcggcgatc tcgccgcgcc gcttgccgtc
      481 ggcgaggtcg gcgaccagct tggcgaggaa cgcctcgtcg gcgctgccca ggtgctgcgg
      541 ctcggcggcc ggcggcggga tcgccggcgg cgccggctcg cggccgacca gccactgcca
      601 gaacgtgcgc ttggggctca tgtcatccga ttcctggggt gctcgggggg gagcggtgtg
      661 gagagcatac ctcggggacg cgggcggaga tcggcgaccc ggctcgccgg acaccggtcg
      721 ccttgccgca gccgtgcagt cgccgtacgg ggccggtgcg tgcatcggcc gagcaagcga
      781 gtccgatggg gcggtcgcgc ggcggtggat aaactcgatg ctggtggatc tcgatggcgc
      841 ggttacagag gcgttgagat atttggtctg tggcgccatg cgcaggcgga tctcacagcc
      901 gcgagtcatt gttgctgtaa ttgcacgctc gttctggtcg tttcgccagt gacaatgatg
      961 acgaatccgg atctgattac atcggccacg ctgtgggcga gaaaacttcg gcaaagtatt
     1021 atcgtggatc ataggcgagg cgagatgtgg tagttgatgg ctgcgattcg tgtggacagc
     1081 agatgtgctc accatcagga gaacatcgat gaccctcgac gagataccgc ctgtccggca
     1141 gccactttcg agccgccgca cgttcacctg gtaccgaccc gccgggcggt cggccacgga
     1201 gtacgaagat ctgacctgcg gccagcagtc ggatcccgcg cactatgcct atcagggctg
     1261 gccgatccgg ttcgatgatg ggcgcgatcc ggtgtacgcc ggttcgacgg cgctgcgctc
     1321 gaccgactgg ttcgcgttcc gcgaccccag ccacacgacg aaccgcgcct acgtagcttc
     1381 gaccaacgag gccgagcgat cgctgtcacg ctcgatcgcc ggggcggtga aggccggcca
     1441 cttcgcgttc gcggagcccg gctgggtgcg cgtcggactg gcgcagcact tcctgaccta
     1501 tccattcgtc aactacggcc tgttcctcgc gttctgctac gccgagcggg aggcgctgtc
     1561 cgacacggtg acattcgcca tcgtgttcac cgccgccgac aagctacgtc acatgcagga
     1621 tgccgtccat tactcgtacg agctcgaagc cgccttcgga acctcggacg ccgaggcgcc
     1681 ggcgcgctgg aaggccgatc cggtgtggca gggagcgcgc gcagcggtcg agcacatcat
     1741 cgcgtcgaac gactggatgg agatcgtgat cgccgcgaat ctgtcgttcg atcgagtgtt
     1801 cggcgatctc gccaaggtcg agtacttcag caggttcgcg gccgccaacg gcgatggcat
     1861 caccccgatg atgatcgcat cggccgcggc cgacgaccac cgtgccacgg cgtggacccg
     1921 ggcgctggtg caccacctgg tgtccgatgc ggcgcacggc gcggcgaacc gcaaacagat
     1981 cagcgaatgg atcgacaagt gggatgcgcg atcgaagcag gcgatcgacg gatttgcgcc
     2041 gtgcttccgc gcggccccgc gcaacccggt gagttttgcc gacgccgcgg ccagcgtgtg
     2101 gcggcgccgt aatcagctcc tggcagagct cgagctcacg ccgagccgga tcagcgtgga
     2161 gggcggcggc catgtctgag atcgccacct caccgctgga acccagggcg cgcgacaccg
     2221 tcggcatctc gctgatgggt agcgaggaga cgactgccgc gctcgagctg gtccgcgccg
     2281 agatgcccga tgcccggatc tccgaccggg gctgcttcta caagatcgag cgcgaggtgc
     2341 tgctcgagct cgacatggcg aagctcggcg agatcctcgg caggccgatc acggtgcacc
     2401 agttcctggt caacatgtcg agctactacg gccgtatcgt catcaacgac ggtctcttgc
     2461 agatccactc tgacatcctg ccggcccggt tccgggattg acgcaaggag gatgcgatga
     2521 cgacacccaa gctggaacaa gtgcacgaga agagcaagcg ctacgactgg ggcttcgact
     2581 atgcgatccc gaaccccaag taccgcactc gctatctgat cccgccccga ggcaaggacc
     2641 cgtttcgcgt cctcatccgt gactacatgg ccatggagaa cgagaaggac aaccgcgtgt
     2701 tcggtgcgct cgacgccgat gtgcgctaca agaatgccga gcgcgccgag ccgcgctggg
     2761 tcgaggcgat gaagttcgcc ttgccggcgt tcaccgacgc cgagtatcag gccgtcaagg
     2821 gcgccgggtt cctcatcacc agcgtgcaga atcaagagct gcgtcaaggc tacgccgcgc
     2881 agatgctcga cgagatccgc cacactcagc tcgagaccgc gctgcgccgt tattacctga
     2941 agaactacca cgacccggcg ggcttcgaca tcggtcagcg tgcgctcggc aaccatccga
     3001 tcggcacgct cgcgcgggcg tcgttccagc cgttcaacac cggcgacccg atcgaggtgg
     3061 cgatgtgcct gaacgtcgtc ctcgagaccg cctacaccaa cccgctggtc gttgcgttgc
     3121 cgcaggccgc cgtggccaac ggcgaccacg cgctggcgac gacgttcctg tcgatccagt
     3181 ccgacgagag tcgccacatg gccaatggct atggcacgct gatgtgctgc gtggcgcagc
     3241 ccgacaacat cccgttcctc aacgagtcgc tggagcgcca cttctggcat cagcacatgt
     3301 cgatggacac gctggttggt gtggcctccg agtactacac ggtgaatcgc ccgtggcgct
     3361 atcgcgatgt ctgggaggag tgggtggtcg acgacttcat cggttcgtac atgacccggc
     3421 tccgggagtt cggcctgcgt ccgccgcagc ggctcccgga ggtcgcgcgg ttcgtcgacg
     3481 acatgcacca ttcggcggcg ctggcgctgg cggcgatctg gcccctcaac ttctggcgca
     3541 tcgacccgct cacggagcgc gactacgatt ggttcgagaa cgcgtatccg ggctggcatg
     3601 cccgctacgg cgggttctgg gacatgtacc gtcggatgtc cgatccggcg tcgggccgga
     3661 tcccgatgca ggtgctgccg tcgctaccgc cgttctgcca ggtgtgccac gcgccgtgcg
     3721 tgatgccgcg cttcgatcag cccgagacgc gggtgttcga gcacggcggc aagcgctacg
     3781 cggtgtgctc cgagggctgc gagtggatct tccgcctcaa cccgacgatc tacaccggct
     3841 gcgccaactg gtgggagccg tggcacggca tgaacctcgc ggatgtcatc gaggcgctcg
     3901 gttacgtacg acccgatggc aggacgctca tggcgcagcc tcacctcggc gcgtcgcgga
     3961 tgtggacgct cgacgacatc cgccgcctcg actacgtgat caaggacccg ctgcggtcct
     4021 gacatggcca gccccccgcc gcgccggatc cgcatcgaac cgtttggtga agcgctggag
     4081 atccgcgccg gcgagtcggt gctcgctgcg atcctgcggc aggggcgctt cgtccgctac
     4141 ggctgcaagc acggcgggtg cggcacgtgc cgcgcgcagc gggtcagcgg cgactgcgag
     4201 ctgtccagcc gcacatcgtt ctcgctgagc gacgccgatc gcgccagcgg cgtggtcctg
     4261 ctgtgctcga cgtacgtcac cagcggggag gcggtgttcg acgtgtcgcg caccatggag
     4321 ctcagcgagg aggagttccg gtccaccggg cgcgtcgccg agtacatcgc cgaggttgcc
     4381 gcgatcgaat cgccgacccc tgacatccgg cgggtcaggt tccagctcgt cgatcccccg
     4441 gcgatgcggt tcaccgcggg gcagtacgtc gaggtggccg tgccgggcac cgacagcgag
     4501 tggcggtcgt attcgatgag cagcgatccg gccgatcgcg gtgctctgga gatcgtcgtc
     4561 aagcggatcc ccggcggccg gttctcctcg gcactcgacg gtcgattgcg ctccggcgac
     4621 cggctccggc tgcgcggccc gatgggccag ttcgcggtcc ggtggtcgca ccggccgatg
     4681 gtcatggtcg ctggcggctc cgggatcggg ccgatccgcg ccatgctgtg ggagctggta
     4741 cggaccggca acgcgcgccc ggtgcacctg ttctacggcg cccggcggcg gagcgatctg
     4801 ttcctcctcg aggagctgcg cgcggtcgag cacgaccagc gctggtttcg gctcacgccc
     4861 acgctgagcg agccggcgcc cggcgccgcc tgggacggcg aggtcgggct cgtcaccgac
     4921 gtgctcgcgc gcgagctccc gcacacgcgg ggcaccgaag cgtatctctg cggctcgcca
     4981 ggcatgatcg acgccgccat ccgcgtcctg atcgccgccg ggtgcaagga gaagcagatc
     5041 tacttcgatc gcttcgttcc gtctggacca tgaccggccg accgcacgat cgggtgcagc
     5101 gccggctgcc ccggcctacg ccacctcgat ctcgtcgccg tcggcgagga tgcggtcctt
     5161 gcggatcagc tggacccgca cgtcgccgag cggctcgtcg ttgaaggtga cggacgtgca
     5221 gctgccgcgg ccgagcagcg ggcggccgcg gtggaagatc aggtcgagcc cgagcccgag
     5281 cggggtcagg tcgagccgct ggtcctcggc gccggcgatc agcgcagcgc cgcggtcgag
     5341 cccgccgcgg acccgcagca cgaggatcgg gccggtgacc tcgtagtcga tcgggcactc
     5401 ctcgcccagg ccgagctcgc cggcgacgcc gagcggcagg cggccggcga gcggcatgcc
     5461 cgcgaggctg gtgccgttgc gcgagtcgag gtcgcgcagc gcgaagccgg cgtcgctgcg
     5521 ctcgatctcg gcgtgctggc gcgacacgcc gccggcgcgc agcgcgaggt cgcacagcgg
     5581 atcgcggccg agcacgatct tcggcgccgc gcacgcgacg atcgccggct tgccgcgccg
     5641 gcgcagctcg acgcgcccgc cggtcagcag cgacgtatcg ag
//
LOCUS       DASVOR010002005         2070 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_109857,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002005 DASVOR010000000
VERSION     DASVOR010002005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109857"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF516_37195"
     CDS             <1..656
                     /locus_tag="VF516_37195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113444.1"
                     /translation="RWGFVAAARPGDDGRQYTKFWENSMRWLIQDPDLRNLHVDSDAV
                     EYAPGQPVRIGVRLLGRDYQPLAGGAVTLTLKRGADPTRAETVATVPITVGEDGTAVH
                     ELGGLSPGVYRALGKATVGGRQVDATDIFLVREGGTELDRPVGDPATLEAIAHATSGV
                     ALGPVDNLPASLAFDPPRIVRVDRRTDVELWSRPGLFVLVIGLLGLEWLLRQRSGYL"
     gene            682..1869
                     /locus_tag="VF516_37200"
     CDS             682..1869
                     /locus_tag="VF516_37200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113445.1"
                     /translation="MSLQLDSLVTDLIFQASAAIDQKTLIDGLGGQDALEGKQMLDVD
                     RLDPRAEMELSPSMPFRRRGERRLMLIDDQAITPRADPQTDSWIRSDLRPAKEALIAM
                     CQHLPVRLGRLFALGSWGDAVIVARSVRDLRGIALIPWALDPQVDIDGKRRQTPLSQK
                     EFEAKLMVYEKRLEELDDPQVIGNLTGASFERRGDLVVVDVLEPDGTWDQRKSLVMET
                     QLAAVDRFSMIPGAPAQPKATDRPTKPIQPPTDKGATKAEAKPEPKGPPITAREIDGK
                     VVLVFPAERFDLDVAAALGKRDWDQVVRRTDNVSGALRDKIHRDGASWIAPLEFLSEV
                     FVEGKPLTKAGFEKSATTAAGVRSMDVHFPRFGPVTLLEIAGKGRFVTSLDSAERAAQ
                     LVP"
BASE COUNT          294 a          787 c          709 g          280 t
ORIGIN      
        1 ggcggtgggg cttcgtcgcc gcggcgcgcc cgggcgacga cggccgccag tacaccaagt
       61 tctgggagaa ctcgatgcgc tggctgatcc aggaccccga cctgcgcaac ctccacgtcg
      121 acagcgacgc ggtcgagtac gcgccgggcc agccggtccg gatcggcgtg cggctgctcg
      181 gccgcgacta ccagccgctc gccggcggcg cggtgaccct gaccctcaag cgcggcgccg
      241 acccgacccg cgccgagacc gtcgcgaccg tgccgatcac cgtcggcgag gacggcaccg
      301 cggtccacga gctcggcggc ctgtcgcccg gcgtgtaccg ggcgctcggc aaggccaccg
      361 tcggcggccg ccaggtcgac gccaccgaca tcttcctcgt ccgcgagggc ggcaccgagc
      421 tcgatcgccc ggtcggcgat cccgcgacgc tggaggcgat cgcgcacgcg acgtccggag
      481 tcgcgctcgg cccggtcgat aacctgccgg cgagcctcgc gttcgatccg ccgcggatcg
      541 tccgcgtcga ccgccggacc gacgtcgagc tgtggagccg gcccggcctg ttcgtgctcg
      601 tcatcgggct tttgggcctg gagtggctct tgcgccagcg cagcggctac ttatagagaa
      661 atccggtaga acagggcatc catgtccctg cagctcgaca gcctcgtcac cgacctgatc
      721 ttccaagcct ccgcggcgat cgaccagaag acgctgatcg acggcctcgg cggccaggac
      781 gcgctggagg gcaagcagat gctcgacgtc gaccggctcg acccgcgcgc cgagatggag
      841 ctgtcgccgt cgatgccatt ccgccggcgc ggtgagcggc gcctcatgct catcgacgac
      901 caggcgatca cgccgcgcgc cgatccacag accgactcgt ggatccgctc cgacctgcgg
      961 cctgccaagg aggcgctgat cgcgatgtgc cagcacctgc cggtccggct cggccggctg
     1021 ttcgcgctcg gcagctgggg cgacgcggtg atcgtcgcgc gcagcgtgcg cgatctccgc
     1081 ggcatcgccc tgatcccgtg ggcgctcgac ccccaggtcg acatcgacgg caagcgccgc
     1141 cagaccccgc tgtcgcagaa ggagttcgag gccaagctga tggtttacga gaagcggctc
     1201 gaggagctcg acgacccgca ggtcatcggc aacctgaccg gcgcctcgtt cgagcgccgc
     1261 ggcgacctcg tcgtggtcga cgtgctcgag cccgacggca cctgggatca gcgcaagtcg
     1321 ctggtcatgg agacccagct cgccgcggtc gaccggttct cgatgatccc cggcgcgccc
     1381 gcccagccca aggccaccga ccgtccgacc aagccgatcc agccgccgac cgacaagggc
     1441 gcgaccaagg ccgaggccaa gcccgagccc aagggcccgc cgatcaccgc ccgcgagatc
     1501 gacggcaagg tcgtcctggt gttccccgcc gagcggttcg acctcgacgt cgccgccgcg
     1561 ctcggcaagc gcgactggga ccaggtcgtc cgccgcacgg acaacgtgtc cggcgcgctg
     1621 cgcgacaaga tccaccgcga cggcgcgagc tggatcgcgc cgctcgagtt cctgtccgag
     1681 gtgttcgtcg agggcaagcc gctgaccaag gccggtttcg agaagtcggc gaccaccgcc
     1741 gccggcgtgc gctcgatgga cgtccacttc ccgcggttcg gcccggtgac cctgctcgag
     1801 atcgccggca agggccggtt cgtcacctcg ctcgacagcg ccgagcgtgc cgcccagctc
     1861 gtgccgtgat ggcggcccgg ctcgccgctc ccatggtcgc cctcggcgcc gcgatcgcgg
     1921 ctgtgatcgc ggtcggcctc accctcggcg ccccctcggc ggcgcgcgcc gatgccgacg
     1981 agttcgacgg cgccagcgtg atcttcgccc gcggctcgtc gctctaccgc gtcgatccgc
     2041 gcggcaaggg cgagatcgag ctcgcccagc
//
LOCUS       DASVOR010002006         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_50883,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002006 DASVOR010000000
VERSION     DASVOR010002006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50883"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VF516_37205"
     CDS             <1..1143
                     /locus_tag="VF516_37205"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8113446.1"
                     /translation="VTVEHVIAPELVDDVAALLLDAQRPLILSGHGVRGGAAPARLIR
                     AAEQLACPVATTPKGKGTFPDAHPLALGVLGLGGHRSAQRYLENGVDVVVAIGTSLGD
                     MSTDGFSPHLQAPRALIHVDIDAKQIGRSYAPTHAIVASAEDFLGALADKLAEAERPA
                     VLRALPGGVERHVLTSSRRFDRIAPHDALAEIQEILPRDTIFSIDSGEHFLFAAHYLE
                     INLPDAFVAMTGLGSMGQSIGAAIGAQLAFPTRTVTAICGDGCFAMNAFEVATAVAER
                     LPIRVFVFNDERLGMVENGHQKVYGRSPAYPTTPMDVCTVAQGLGATVLRVERPGQLL
                     AASDLLCSSPGPVVVDVRIDPEIILPRRDRVSAMAPGGAGKTKQVK"
     gene            1140..>1827
                     /locus_tag="VF516_37210"
     CDS             1140..>1827
                     /locus_tag="VF516_37210"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113447.1"
                     /translation="MSGPRIGLLAAATYLPPEIRRNDWWPSATVERWMALRAAPPPPP
                     AGALTPAMAHVLAEMAGQAVDPFQGVTERRVMPADMTSVDMEVQAAERAIAHAEATAG
                     PVTGFDRRQIDVLLTHTAVPEYLLSNTACVLHHRLGLAPTCFTMQAEASGNSFLMQLT
                     VAEQMITAGRARHALVVQSAAGSRLLDPDSPMAPLFGDGASAVVIGRVPDGGLLATVH
                     RTDGSHPRALI"
BASE COUNT          232 a          700 c          647 g          248 t
ORIGIN      
        1 gtgaccgtgg agcacgtcat cgcgcccgag ctggtcgatg atgtcgccgc gctgctgctc
       61 gacgcccagc gcccgctgat cctgtcgggg cacggcgtgc gcggcggcgc cgctcccgcg
      121 cgcctgatcc gcgccgccga gcagctggcc tgcccggtgg cgaccacgcc caagggcaag
      181 ggcacgttcc ccgacgccca tccgctggcg ctcggcgtgc tcgggctcgg cggccaccgc
      241 tcggcccagc gctacctcga gaacggggtc gacgtcgtgg tcgcgatcgg caccagcctc
      301 ggcgacatgt cgaccgacgg gttctcgccc cacctccagg cgccgcgcgc gctgatccac
      361 gtcgacatcg acgccaagca gatcggccgg agctacgcgc cgacccacgc gatcgtcgcg
      421 tccgccgagg acttcctcgg cgcgctcgcc gacaagctgg ccgaggccga gcggcccgcc
      481 gtgctgcgcg cgctgcccgg cggggtcgag cgccacgtgc tgacctcgtc gcggcggttc
      541 gaccgcatcg ccccgcacga tgcgctcgcc gagatccagg agatcctgcc gcgcgacacg
      601 atcttctcga tcgactcggg cgagcacttc ctgttcgccg cccactacct ggagatcaac
      661 ctgcccgacg cgttcgtcgc gatgaccggg ctgggctcga tgggccagtc gatcggcgcg
      721 gcgatcgggg cgcagctcgc gttcccgacc cgcaccgtca ccgcgatctg cggcgatggc
      781 tgcttcgcga tgaacgcgtt cgaggtcgcg accgcggtcg ccgagcggct gccgatccgg
      841 gtgttcgtgt tcaacgacga gcggctcggc atggtcgaga acggccacca gaaggtctac
      901 ggtcgcagcc cggcgtaccc cacgacgccg atggacgtgt gcacggtcgc acagggcctg
      961 ggtgcgaccg tgctgcgggt cgagcgcccg ggtcagctgc tcgccgcgag cgatctgctg
     1021 tgctcgtcgc ccggcccggt cgtggtcgat gttcgcatcg atcccgagat catcctgccc
     1081 cggcgcgacc gggtcagcgc gatggcgccc ggcggcgccg gcaagaccaa gcaggtcaag
     1141 tgagcgggcc ccggatcggc ctgctcgccg ccgcgacgta ccttcccccg gagatccggc
     1201 gcaacgactg gtggccgagc gcgacggtcg agcgctggat ggcgctgcgc gcggcgccgc
     1261 ccccaccgcc ggccggcgcg ctgaccccgg cgatggccca cgtgctcgcc gagatggccg
     1321 ggcaggcggt cgatccgttc cagggggtca ccgagcgccg cgtgatgccg gccgacatga
     1381 cctcggtcga catggaggtg caggccgcgg agcgcgcgat cgcccacgcc gaggcgaccg
     1441 ccgggccggt caccggcttc gaccgccgcc agatcgacgt gctgctcacc cacaccgcgg
     1501 tgcccgagta cctgctgagc aacaccgcgt gcgtgctgca ccaccggctc ggcctcgcgc
     1561 cgacctgctt cacgatgcag gccgaggcgt cgggcaactc gttcctgatg cagctcaccg
     1621 tcgccgagca gatgatcacc gccggccggg cgcgccacgc gctggtcgtg cagtccgccg
     1681 ccgggtcgcg gctgctcgac cccgacagcc cgatggcgcc gctgttcggc gatggcgcgt
     1741 ccgcggtggt gatcggccgc gtgccggacg gcgggctgct cgccaccgtg caccggaccg
     1801 acggctcgca cccgcgcgcg ctgatcg
//
LOCUS       DASVOR010002007         2166 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_255551,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002007 DASVOR010000000
VERSION     DASVOR010002007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255551"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="VF516_37215"
     CDS             <1..1253
                     /locus_tag="VF516_37215"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8113448.1"
                     /translation="IVRVGDPVEVAADPRAAGARRIAWPGRAMVPGTVNTHNHSFQSL
                     LRGIGDDLPFLEWRDRALYRYSPRLDADDMATAALFAFGEMLLHGVTTVCDFYYLNRG
                     GNDHASATIEAARRLGIRIVLARCFYDWTGAPEAYRETVPQAVANFEALHRRYHDRDR
                     HRVSIQPAPHSQHGATPAMIEAGAGCARDAGVPWHIHLAEERYQVDQSLERFGARPLH
                     AVAALPVDLAQMIAVHGCWFDAGERAVLAERGGALAYCPGSNLFLGDGVTDLVDLVAR
                     GVPVGLGTDGGCSNNRVSVFDEMRTCALLQKVHRTDGQAIDAETCFALGTRTAGAVLR
                     LPIGRIAPGYRCDLVALDLDDPSLWPVQALEKNVVYSLSPRAISDVVVDGQEVVAARR
                     LCHVVLDEIQARVRDVTDHWRGER"
     gene            1279..1887
                     /locus_tag="VF516_37220"
     CDS             1279..1887
                     /locus_tag="VF516_37220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113449.1"
                     /translation="MTDEASPSTATARADADAPARNGGAAPHNAADRPRGERSGSRPA
                     RATQIELEVSRQRSIAWTIFGLIVGALLIHGLGTVGVWVGIALVAAGLYHAWQLVQTL
                     LHPPGTIAVSEAEVVLPRGLCMPRPVVVAPRDITAIYFLRRSVPWNRAAPVLIIELGE
                     RAMAFPRDWFASEADQRHVIHAVLRAKGEAPAPASSGDPDAA"
     gene            complement(1922..>2166)
                     /locus_tag="VF516_37225"
     CDS             complement(1922..>2166)
                     /locus_tag="VF516_37225"
                     /inference="COORDINATES: protein motif:HMM:NF013360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016641 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, oxygen as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lysyl oxidase family protein"
                     /protein_id="HEX8113450.1"
                     /translation="FCLEDVENIQPGIPPTGYSCSDQGITRGWADVYSRFLPCQWIDI
                     TGVPAGTYTLRVVVNPLRTLPESNYDNNTFTVLVHF"
BASE COUNT          286 a          814 c          758 g          308 t
ORIGIN      
        1 cgatcgtccg ggtcggcgat cccgtcgagg tcgccgccga cccgcgcgcc gccggggcgc
       61 gccggatcgc gtggccgggc cgcgcgatgg tccccggcac cgtcaacacc cacaaccaca
      121 gcttccagtc cctgctgcgc ggcatcggcg acgacctgcc gttcctcgag tggcgcgatc
      181 gcgcgctgta ccgctactcg ccccggctcg acgccgacga catggcgacc gcggcgctgt
      241 tcgcgttcgg cgagatgctg ctccatggcg tcaccaccgt gtgcgacttc tactacctca
      301 accgcggcgg caacgaccac gcgtcggcga cgatcgaggc cgcccgccgg ctcggcatcc
      361 gcatcgtgct cgcgcgctgc ttctacgact ggaccggcgc gcccgaggcc tatcgcgaga
      421 ccgtgccgca ggcggtggcc aacttcgagg cgctgcaccg ccgctaccac gaccgcgacc
      481 gccaccgggt cagcatccag cccgcgccgc acagccagca cggcgcgacc ccggcgatga
      541 tcgaggccgg cgccggctgc gcgcgcgacg ccggcgtgcc gtggcacatc cacctcgccg
      601 aggagcgcta ccaggtcgac cagtcgctcg agcggttcgg ggcgcggccg ctccacgccg
      661 tcgcggcgct gccggtcgat ctggcgcaga tgatcgcggt ccacggctgc tggttcgacg
      721 ccggcgagcg cgccgtgctc gccgagcgcg gcggcgccct cgcctactgc ccgggctcga
      781 acctgtttct cggcgacggc gtgaccgacc tggtcgacct ggtcgcgcgc ggcgtgccgg
      841 tcggcctggg caccgacggc ggctgctcca acaaccgggt cagcgtgttc gacgagatgc
      901 gaacctgtgc gctgctccag aaggtccacc gcaccgacgg ccaggcgatc gacgccgaga
      961 cctgcttcgc gctcggcacc cggaccgcgg gggccgtcct gcggctgccg atcggtcgga
     1021 tcgccccggg ctaccgctgc gacctggtcg ccctcgatct ggacgatcca tcgctgtggc
     1081 cggtccaggc gctcgaaaag aacgtggtgt actccctgtc gcctcgcgcg atcagcgacg
     1141 tcgtggtaga tggtcaggag gtcgtcgccg cgcggcgcct gtgtcatgtc gtcctcgatg
     1201 agatccaagc ccgcgtccga gacgtcacag atcactggcg tggagaacga tgagcggcac
     1261 caggcgcagg aggagagcgt gactgacgag gcctcgccgt ccaccgccac ggccagggcc
     1321 gacgccgatg cccctgcgcg gaacggcggc gcggcgccgc acaacgcggc agatcgtccg
     1381 cgcggcgagc gctccggatc caggcccgcc cgggccacgc agatcgagct ggaggtctcc
     1441 cgccagcgct cgatcgcctg gaccatcttc ggcctgatcg tcggggcgct tctgatccat
     1501 ggcctgggca ccgtcggcgt ctgggtcggg atcgcgctgg tcgcggccgg cctctatcac
     1561 gcctggcagc tggtccagac cctgctccat ccgcccggca ccatcgcggt ctccgaggcc
     1621 gaggtcgtgc tgccccgcgg gctgtgcatg ccgcgaccgg tcgtggtcgc cccgcgcgac
     1681 atcaccgcga tctacttcct gcgccgctcg gtgccgtgga accgcgccgc gccggtgctg
     1741 atcatcgagc tcggcgagcg cgccatggcg ttcccgcgcg actggttcgc ctcggaggcc
     1801 gaccagcgcc acgtgatcca cgcggtgctg cgcgccaagg gcgaggcccc ggcccccgcg
     1861 agctccggcg accccgacgc ggcctgagcc ccggaggtcg atcgcccgcc gaccgcggcg
     1921 ctcagaagtg cacgaggacg gtgaacgtgt tgttgtcgta gttcgactcg ggcagcgtgc
     1981 gcagcgggtt gacgacgacg cgcagcgtgt acgtgcccgc cggcacgccg gtgatgtcga
     2041 tccactggca cggcaggaac cggctgtaga cgtcggccca gccgcgggtg atgccctgat
     2101 cgctgcacga gtagccggtc ggcggtatgc cgggctggat gttctcgacg tcctcgaggc
     2161 agaacg
//
LOCUS       DASVOR010002008         2274 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_459413,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002008 DASVOR010000000
VERSION     DASVOR010002008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459413"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..304
                     /locus_tag="VF516_37230"
     CDS             164..304
                     /locus_tag="VF516_37230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113451.1"
                     /translation="MAGDGLIARDALSQTIAAVAELEHQACADRPGADDRRSSAATGQ
                     RF"
     gene            830..1045
                     /gene="lepB"
                     /locus_tag="VF516_37235"
     CDS             830..1045
                     /gene="lepB"
                     /locus_tag="VF516_37235"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEX8113452.1"
                     /translation="MAKQNAGACELQMHYVVPKDHVFVLGDNRPNSNDSRYWGSVPVA
                     QIKGKAFGIWFSAGRAGARWDRFGGFQ"
     gene            1275..1709
                     /locus_tag="VF516_37240"
     CDS             1275..1709
                     /locus_tag="VF516_37240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113453.1"
                     /translation="MAKRNSTRRSAAGRPRASAKHAPPAPAKQSLRERIQQQLGDTPI
                     DDSLAAVTWMQRVLAVVASDLIESELDDHKKRSEMLKIADRMAKLRDIDRLHQAEQTV
                     RGEKKQQEDGPAGPQLIDAPRLDAAAGPITARRGRPPRRTVS"
     gene            complement(1985..>2274)
                     /locus_tag="VF516_37245"
     CDS             complement(1985..>2274)
                     /locus_tag="VF516_37245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8113454.1"
                     /translation="HRARAHLDVAAGADLPDPRPSPGEGLDDAELQAALVTCLATLDD
                     PARTAVLLRYQQGLTYEEMAEICGEKAGTLQARVARALRKLRDRLEGQLAG"
BASE COUNT          354 a          807 c          796 g          317 t
ORIGIN      
        1 tcatccgctt ccacctgtcc ggatcaaagt gcagatctgg aacactccgt aagacgagcc
       61 gacagaagag ccggatggtg gcaatctcct tgtccggatc tggcgagggc cccggtcggc
      121 aacggccggg gctactctac aatcggggtt acccgggccc aagatggccg gcgacggcct
      181 gatcgcgcgg gatgcactct cgcagacgat cgctgcggtc gctgagctcg agcaccaggc
      241 ctgcgccgac cggccaggcg ccgacgatcg ccgcagctcg gcggcaaccg gtcagcgctt
      301 ctgacggcga atccggtgga cctgatgcac ccgtgcgctg cgctatgatg ccgcgcatgg
      361 cgactcgagg accggcgcgt gccattgtcg ggaccgtact cagcgcgtgg ctggtcgggc
      421 ccggggccgg cgcagggctc gtcgggcgat acggcctgat gctggcatgg atcgcggcgt
      481 tcgccgcggc cctggtcgcc tccacgctct cggtgtggct gctcgtcctg ccgctcgcag
      541 tctggatcgc cgcagtgatc cgcgcgtatc ccacagtgcg cgcggccgac cgcgccggcg
      601 cccggacgag cgtgatcggc atcgtcaccg cgttcgtgcg caacctcgcg gtcgcgatcg
      661 tcgtccgggc gacggccgtg gaggcgttcc ggatcccgtc gtccgccatg gagccgaccc
      721 tcgtcatcgg cgaccacctc ttcatcaaca agttcatcta cggcatccgg atccccttca
      781 cgaccgccgg ccgtcgggca cccacgacca ggcgcccggc gagatcgtcg tggccaagca
      841 gaacgcgggc gcgtgcgagc tccagatgca ttacgtcgtg ccgaaggacc acgtgttcgt
      901 tctgggcgac aaccggccca actcgaacga ctcgcgctac tggggctcgg taccggtggc
      961 gcagatcaag ggcaaggcgt tcgggatctg gttctccgcc ggccgggccg gcgcacgctg
     1021 ggaccggttc ggcggcttcc agtgacaccg ctcgcgaggc agcagccacc ggtgcgcgcc
     1081 gcgacggcga actaggtgcc gtgcagggca tagattcgcg agcgtcagcg cctgcgcatg
     1141 gtccgcgcgg cttgcaaagg tgatcgtcgc tgcgatcatg caggcgcatt ttcatgggcg
     1201 aggcgccggc tggtctgacc ccggtggccg gagggggtct ggcagcgcca cacaccgccc
     1261 cgtagcgtca tcctgtggcc aagcgcaaca gcacgcggag atcggcggcc gggcgacccc
     1321 gcgcctcggc gaagcacgcg ccgccggcgc cggcgaagca gtcgctgcgc gagcgcatcc
     1381 agcagcagct cggcgacacg ccgatcgacg actcgctggc cgcggtcacc tggatgcagc
     1441 gcgtgctcgc cgtggtcgcc tccgacctga tcgagtccga gctcgacgac cacaagaagc
     1501 gcagcgagat gctcaagatc gccgaccgga tggcgaagct ccgcgacatc gaccggctgc
     1561 accaggccga gcagacggtt cgcggcgaga agaagcagca agaggacgga cccgcgggtc
     1621 cgcaactgat cgatgccccg cgcctcgacg cagcagctgg tccgatcaca gctcgacgcg
     1681 gccgcccgcc acgaagaacg gtatcttgac ctgcagatcg actgggtcca cgagaagaac
     1741 gggacccaca tcgcgcggtt cggaggcctg tgggatcgcc gcacgaaggc gttcgtcggc
     1801 gacgcggcgc gctcccacgt gatcgcaacg ccaaagaccc cgccgaccct cacgagcccg
     1861 ctgatcctgc agcgtcctga cggccgctgg gcgatcctgt tcgacgccgc gcagcccgcg
     1921 gcaatgccgc cggccaagaa gatcaagccg gagggcacga cgtacacggt cgagcgtgag
     1981 ccggttaccc cgcgagctgg ccctcgagcc ggtcgcgcag cttgcgcagc gcgcgcgcca
     2041 cccgggcctg cagcgtgccg gccttctcgc cgcagatctc ggccatctcc tcgtaggtca
     2101 ggccctgctg gtagcgcagc agcacggcgg tccgggccgg atcgtcgagc gtggcgaggc
     2161 aggtcaccag ggcggcctgg agctcggcgt cgtcgagccc ctcgccgggc gatggccgcg
     2221 gatcgggcag gtcggcgccg gcggcgacgt cgaggtgcgc gcgcgcccgg tgcc
//
LOCUS       DASVOR010002009         4299 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_400441,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002009 DASVOR010000000
VERSION     DASVOR010002009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4299
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400441"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VF516_37250"
     CDS             <1..231
                     /locus_tag="VF516_37250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113455.1"
                     /translation="GRTADEARSRGERRETSLDVAVSCHALPDGRVDWVLSSSHAAWS
                     VGTVRRLADALWPGAEVVAGRTPGVVHVITGP"
     gene            complement(530..967)
                     /locus_tag="VF516_37255"
     CDS             complement(530..967)
                     /locus_tag="VF516_37255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113456.1"
                     /translation="MSDLIDDRAVDALQIVWDTSCAGPRVVDSPPELRMIQEFLSRDT
                     WQHFVQFILPGVVGHHQRVAEYSGFRFQSDLDPDDDPFEGVEMFDPIDTIHISEAAFD
                     RLMSRYFDTIVRGATENLRPEIRESWWPKIVDGARTLGDRARP"
     gene            complement(967..>4299)
                     /locus_tag="VF516_37260"
     CDS             complement(967..>4299)
                     /locus_tag="VF516_37260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8113457.1"
                     /translation="FTVGNHVAFAPGRYSPGTVEGDALLAHELTHVMQQRGATPRDGA
                     GSGSAGAEVDADRGALAAVNHLHGAGTEQAAPSLTADFQLQRCSGDPAETGLMATDFK
                     GKFLGKQVGESKTTVNAGIPSTVNFKLMAARTLGAFDTEAAAIAVVKTNGKPGAVTVE
                     DGKFVGYETDLGFGYRTTTSGNMAGGSSTEYAPKLSPGVIALVSNEGMTVRAADFDPS
                     AKDPAAGNADAAMQSTDNPVAGYRQALGDKTGDLNGLPDDKLLAAFNGALKDTALVVL
                     NKSAAEVKNKQARFGTGGGGISDTEYETMKQTAAQLAELDPKIATAQKTLDSLTLSRA
                     MTAASGDGVVPVDTSDFDADIDAAHKELAALQTQRKIQIARYPLLSRVDPARFKDKSK
                     DDMAKELGGNLPGILQDIETTKQNVADGKINLWEVEQVVDAAVAGFGLSPERRKLIDD
                     TRASEAKKKTIETVVFTVFAIGFGVAAMFATGGAGLFFAAGAFGLSTYDAMKQTEQYM
                     VDKPASNIAEDKDGGFNAPPGWGWLVVAWIGVGLDATQVVSAVGKVAKTGKVLEGAED
                     LAKAENAAKVGMTREALLAKLRQVAGEVDAAATLTEGTRATVAQRLGILVDIDPKLGG
                     DVRILYEVDKTTGRAIVKSLSAGPEAKLVEVLAHENVVKLMRRYEGATGRLREIWEKL
                     LAFVGKSPKDANPFPAGSKAYESWNELQKMPELVEVTKLKYADGIGKANESALAKDLE
                     ILEAEARRHQKIVDEMVLEAGDGFVAKTGDSTRAALDAKYPRPPDCKTAEDMIAKGYY
                     YRLGANGNYEIARTSKSVGQQLRVEFDATGKIPVRLVAAVEKLEARIPDAAQLARLRT
                     IVTDDARLSKLYDAVLDANRLEKLLNDAGDVGKLEKLLGDVGKAEDLEKLLKHMTISE
                     LEKFMTELGDASKMKGLAEKYGGDVLKHYGGKFFKDFKGVDSGTLNHLATFDGIDKKV
                     GIKGCHDDALFRSELAKPPDGVKSPPAGVGRGEIISEVPHPSDPRVKKIEYGFWQQDG
                     KGNLVSPLTRKAASYTKTTIVGLAGDTATWQAQAMEAVEDAIKAMTFPVGDASFSGVA
                     KNGMKWTGWYRSGKIQTVFVDF"
BASE COUNT          593 a         1488 c         1417 g          801 t
ORIGIN      
        1 ggccgcacgg ccgacgaggc ccgaagccgc ggcgagcgcc gcgagacctc gctcgatgtc
       61 gcggtcagct gccacgccct ccccgacggc cgcgtcgact gggtgctgtc gtcgagccac
      121 gccgcatggt cggtcggcac cgtgcggcgg ctcgccgacg cgctgtggcc tggcgccgag
      181 gtcgtggccg gtcgcacccc cggcgtcgtc cacgtgatca ccgggccgtg atcgagaact
      241 gcgcccagtt ctcgcgctgg cagatgggac agcgccactg attgagcagg cgagtgcgag
      301 catcgtcgcg gaaggcagcc cgagtccgcg atatcatcgt cgccgaggtc gcgcgggtcg
      361 agctggtacc ccacgccggg ctcgactccg cgcgcatcgt cgcagaggtg ggtctgcatg
      421 ttcgttgccg aggtcccggg ctgaccgctg cgcagttcgg gcacttgagg gctgctacga
      481 agtagtcgta cattggtgct ccgcatgcgc ggtggcgggc caactagact cacggtcgtg
      541 cgcggtcacc gagcgtgcgc gcaccgtcga cgatcttggg ccaccacgac tcgcgaatct
      601 ccgggcgcag gttctcggtc gctccgcgga cgattgtgtc gaagtaccga ctcatcaggc
      661 ggtcgaacgc ggcctcgctg atgtggatcg tgtcaatcgg atcgaacatc tcgacgccct
      721 cgaacggatc gtcgtcgggg tcgaggtcac tctggaagcg gaacccgctg tactccgcga
      781 cgcgctggtg gtgaccaacg actcccggca ggatgaattg cacaaagtgc tgccaggtat
      841 cacgagacag gaactcctga atcattcgca actcgggggg ggagtccacg acgcgcggcc
      901 cggcgcagct cgtgtcccag acgatctgaa gcgcgtcgac ggcgcggtca tcaatgaggt
      961 cgctcatcag aagtccacga agacggtttg gatcttaccg ctacgatacc acccggtcca
     1021 cttcatgccg ttcttggcga cgccagagaa cgacgcgtcc ccgaccggga acgtcatcgc
     1081 cttgatcgcg tcctcgaccg cctccatcgc ctgtgcttgc cacgtagccg tatcgcccgc
     1141 aaggcccacg atcgtggtct tcgtgtagct ggctgccttg cgcgtgagcg gcgagaccag
     1201 gttgccctta ccgtcctgct gccagaagcc gtactcgatc ttcttcacgc gcgggtccga
     1261 cgggtgcgga acctcggaga tgatctcgcc gcgcccaaca cccgcgggcg gcgacttgac
     1321 accgtcgggt ggtttcgcca gctcgctccg gaacagcgca tcgtcgtgac agcccttgat
     1381 gccgaccttc ttgtcgatcc cgtcgaaggt cgccagatgg ttcaacgtgc ccgaatcgac
     1441 gcccttgaag tccttgaaga acttaccacc gtagtgcttg agcacgtcgc cgccgtactt
     1501 ctcagcgagc cccttcatct tgctcgcgtc accgagctcg gtcatgaact tctcgagctc
     1561 cgagatggtc atgtgcttga gcagcttctc gagatcctcg gccttgccca cgtctccgag
     1621 cagcttctcg agcttgccga cgtcgccagc gtcattgagc agcttctcta gacggttcgc
     1681 gtcgagcacc gcgtcgtaga gcttcgagag ccgggcgtcg tcggtgacga tcgtgcgcag
     1741 ccgcgcgagc tgcgccgcat ccgggatcct cgcctcgagc ttctcgacgg cggcgacgag
     1801 ccggaccggg atcttaccgg tggcgtcgaa ctcgacccgg agctgctggc cgacgctctt
     1861 cgacgtacgc gcgatctcgt agttgccgtt cgcgccgagc cggtagtagt agcccttcgc
     1921 gatcatgtcc tcggcggtct tgcagtccgg tggccgcggg tacttcgcat cgagcgctgc
     1981 gcgcgtgctg tcgccggtct tcgccacgaa gccgtcgccg gcctcgagca ccatctcgtc
     2041 gacgatcttc tggtggcggc gcgcctcggc ctcgaggatc tcgaggtcct tggcgagcgc
     2101 gctctcgttc gccttgccga tcccgtcggc gtacttgagc ttcgtcacct cgacgagctc
     2161 cggcatcttc tggagctcgt tccacgattc gtaggccttg gagccggccg gaaacgggtt
     2221 cgcgtccttc gggctcttgc cgacgaacgc gagcagcttc tcccagatct cgcggagccg
     2281 gccggtggcg ccctcgtagc gccgcatcag cttcaccacg ttctcgtgcg cgaggacctc
     2341 gacgagcttg gcctcggggc ccgccgagag gctcttcacg atcgcgcggc ccgtggtctt
     2401 gtcgacctcg tagaggatcc ggacgtcgcc gccgagtttg ggatcgatgt cgacgaggat
     2461 gccgagccgc tgggcgaccg tggcgcgcgt tccctcggtg agggtggcgg cggcgtcgac
     2521 ctcgccggcg acctggcgca gcttcgcgag cagcgcctcg cgcgtcatgc cgaccttcgc
     2581 agcgttctcg gcctttgcga gatcctcggc gccctcgagc accttgccgg tcttggcaac
     2641 cttgccaacg gcgctcacca cctgcgtggc gtcgaggccg acgccgatcc acgccacgac
     2701 cagccagccc cagcccggcg gggcgttgaa tccgccgtcc ttgtcctcgg cgatgttcga
     2761 cgcgggcttg tcgaccatgt actgctcggt ctgcttcatc gcgtcgtacg tgctcagccc
     2821 gaacgcgccg gccgcgaaga acagcccggc gccgccggtc gcgaacatcg cggcgacgcc
     2881 gaagccgatc gcgaacacgg tgaacacgac ggtctcgatc gtcttcttct tggcctcgga
     2941 cgcgcgggtg tcgtcgatca gcttgcgtcg ctcggggctg agcccgaacc cggcgaccgc
     3001 cgcgtcgacg acctgctcga cctcccacag gttgatcttg ccgtcggcga cgttctgctt
     3061 ggtcgtctcg atatcctgaa ggatgcccgg caggttgcca ccgagctcct tcgccatgtc
     3121 gtccttggac ttgtccttga acctggccgg atcgacgcgc gacagcagcg gataccgagc
     3181 gatctggatc ttgcgctggg tctgcagcgc cgcgagctcc ttgtgcgccg cgtcgatgtc
     3241 ggcgtcaaaa tccgacgtgt cgacggggac gacgccgtcc cccgacgcgg cggtcatcgc
     3301 cctcgacagc gtgaggctgt cgagcgtctt ctgtgcggtg gcgatcttcg gatcgagctc
     3361 ggcgagctgc gccgccgtct gcttcatcgt ctcgtactcg gtgtcgctga tccctccgcc
     3421 gccggtcccg aatctggcct gcttgttctt gacctccgcc gcactcttgt tgagcacgac
     3481 gagcgcggtg tccttgagcg cgccgttgaa cgcggcgagc aacttgtcgt cgggcaggcc
     3541 gttgagatcg ccggtcttgt cgccgagcgc ctggcgatac ccggcgaccg gattgtcggt
     3601 cgactgcatc gcggcgtcgg cattgcccgc cgccgggtcc ttcgccgagg ggtcgaagtc
     3661 cgcggcgcgg accgtcatgc cttcgttcga gaccagcgcg atcacacccg gcgacagctt
     3721 cggcgcgtac tcggtgctgc tcccgccggc catgtttccc gacgtcgtgg tgcgatagcc
     3781 gaagccgaga tcggtctcgt agccgacgaa cttgccgtcc tcgacggtca ccgcgcccgg
     3841 cttgccgttg gtcttgacca ccgcgatcgc ggcggcctcg gtgtcgaacg cgccgagggt
     3901 gcgcgcggcc atgagcttga agttcacggt cgacgggatc ccggcgttca ccgtggtctt
     3961 gctctcgccg acctgcttgc cgaggaactt gcccttgaag tccgtggcca tcagcccggt
     4021 ctcggcgggg tcgccgctgc agcgctggag ctggaagtcc gccgtcagcg acggcgcggc
     4081 ctgctcggtg ccggcgccgt gcaggtggtt caccgccgcg agcgcgccgc gatcggcgtc
     4141 gacctcggcg ccggccgagc cggagcccgc accgtcgcgc ggggtcgcgc cgcgctgctg
     4201 catcacgtgg gtgagctcgt gggcgagcag cgcgtcgccc tcgacggtgc ccggactgta
     4261 gcggcccggc gcgaacgcga cgtgattgcc gacggtgaa
//
LOCUS       DASVOR010002010         3424 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_313904,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002010 DASVOR010000000
VERSION     DASVOR010002010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_313904"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..810)
                     /locus_tag="VF516_37265"
     CDS             complement(34..810)
                     /locus_tag="VF516_37265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113458.1"
                     /translation="MVAPWRRDSYVFGGGPAEVALIALSSVKLPDPLPVSRGRHGMPD
                     DGEGALAVALVARDRAEDPAWFAGQVVAPFADLIATDLGPEAAVAALAAAHAYVIEAR
                     VDDPDDLGHLQGAWALAKCVCEEGAAVVIDVHAARAHLGSDVAALAPDRSLDIMHEVT
                     LFFDEQPDGLLAAWSLGLRKFGRPDLVLLGVPPEAATEAALVLRDVAATLADGERIEP
                     GDQIAAPDGRTLTAARFDPARSPVVAVDGPALVLAAAPPG"
     gene            complement(813..2618)
                     /locus_tag="VF516_37270"
     CDS             complement(813..2618)
                     /locus_tag="VF516_37270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8113459.1"
                     /translation="MADRDTIPHRLLRQAATNPSTIAYQSKIDGRWQPTTWKTVADHV
                     RTAARALIALGFPRGGKVAILGFNRLEWVILDHAAMMAGGSAAGIYTTCSPDEVQYII
                     HHSEAHLVLVEDAGQLAKVIARRDQLPLLRWIVVMRGTTTEPPAPASGEGILSWEAFN
                     AKGEATAAGDAELDARLAALQPSDLATLIYTSGTTGPPKGVMLSHANLAWTSQLLIDV
                     GGGRVAGDTSLSYLPLSHIAEQTGTIHMPVTAGSTVYFAESIDKLADNIKECRPTVFF
                     GVPRVWEKFHAVLSARLAEVTGGKRRLLDWSRRTCSELHARRDRGDPVPWLLRTQYAL
                     ADRLVLSKLKAALGLDRARVLSSGAAPIAPDVLAFFASIDLPIREIYGQSEDTGPTSF
                     NLPGRTRIGSVGPPLPGIEVKIAEDGEILVRGPNVFMGYYKEPQATAETLKDGWLCSG
                     DLGAIDKDGFLTITGRKKEIIITAGGKNIAPKNIEAALKESLLISEAIVIGDRKKFLS
                     ALITIDEAVAQKLAPDVAAGQLAASPAVRAAVQQQVDTVNETLARVEQIKKFTILPQP
                     LSVATGELTPTLKLKRKVIAQRYEAQINAMYADGD"
     gene            complement(2627..3010)
                     /locus_tag="VF516_37275"
     CDS             complement(2627..3010)
                     /locus_tag="VF516_37275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113460.1"
                     /translation="MQGFVAQITELARRAAIDTLESAFGGRAGRSAPAAEALAVNLGR
                     VGRPRGGRGAKRSSADLEALSQRFASFVSANPGLRIEQINKQLGTTTKDLALPIRKLI
                     SEGRIKAKGQKRSTTYFPGKKKGKS"
BASE COUNT          485 a         1197 c         1222 g          520 t
ORIGIN      
        1 agcgaatcga acgcggaccc ggcgcgaccg ggatcagccc ggcggggccg cggcgagcac
       61 cagcgccggg ccgtcgaccg ccacgaccgg cgacctcgcc gggtcgaacc gggccgcggt
      121 gagggtgcgg ccgtcggggg cggcgatctg atcgccgggc tcgatccgct cgccgtcggc
      181 cagggtcgcc gccacgtcgc gcagcaccag cgcggcctcg gtggccgcct cgggcgggac
      241 cccgagcagg acgaggtccg ggcggccgaa cttgcgcagg ccgagcgacc acgcggcgag
      301 caggccgtcg ggctgctcgt cgaagaacag cgtgacctcg tgcatgatgt cgaggctgcg
      361 gtcgggggcg agcgcggcga cgtcgctgcc gaggtgggcg cgcgcggcgt ggacgtcgat
      421 caccaccgcc gcgccctcct cgcacacgca cttggcgagc gcccacgcgc cctggaggtg
      481 gccgaggtcg tccggatcgt cgacccgcgc ctcgatcacg taggcgtgcg ccgcggcgag
      541 cgcggcgacc gccgcctcgg ggccgagatc ggtcgcgatc agatcggcga acggcgccac
      601 cacctggccg gcgaaccacg ccggatcctc ggcgcgatcg cgggcgacca gcgccaccgc
      661 gagggcaccc tcgccgtcgt cgggcatgcc gtggcggccg cgcgacaccg gcagcggatc
      721 cggcagcttg accgacgaca gcgcgatcag cgcgacctcc gccgggccgc cgccgaacac
      781 gtagctatcg cggcgccacg gcgcgaccac cgctagtcgc cgtccgcgta catcgcgttg
      841 atctgggcct cgtagcgctg cgcgatcacc ttgcgcttga gcttcagcgt cggggtcagc
      901 tcgccggtgg cgacgctgag cggctggggc aggatcgtga acttcttgat ctgctcgacc
      961 cgcgcgaggg tctcgttgac cgtgtcgacc tgctgctgga ccgccgcgcg gaccgcgggc
     1021 gacgcggcga gctggccggc cgcgacgtcc ggggcgagct tctgcgcgac cgcctcgtcg
     1081 atcgtgatca gcgcgctgag gaacttcttg cggtcgccga tcacgatcgc ctcgctgatc
     1141 agcagcgact ccttgagcgc ggcctcgatg ttcttgggcg cgatgttctt gccgcccgcg
     1201 gtgatgatga tctccttctt gcggccggtg atcgtgagga agccgtcctt gtcgatcgcg
     1261 ccgaggtcgc ccgagcacag ccagccgtcc ttgagcgtct cggcggtcgc ctgcggctcc
     1321 ttgtagtagc ccatgaacac gttggggccg cgcaccagga tctcgccgtc ctcggcgatc
     1381 ttgacctcga tgccgggcag cggcggaccg accgagccga tccgggtccg cccgggcagg
     1441 ttgaacgacg tcgggccggt atcctcgctc tggccgtaga tctcgcggat cggcagatcg
     1501 atgctcgcga agaacgcgag cacgtcgggg gcgatcggcg ccgcgcccga gctcagcacg
     1561 cgcgcgcggt cgaggccgag cgcggccttg agcttgctca gcaccagccg gtcggccagc
     1621 gcgtactggg tccgcagcag ccacggaacc ggatcgccgc gatcgcgccg cgcgtggagc
     1681 tcgctgcacg tccggcgcga ccagtcgagc agccggcgct tgccgccggt gacctcggcc
     1741 agccgcgccg acagcacggc gtgaaacttc tcccacaccc gcggcacgcc gaagaacacc
     1801 gtcgggcggc actccttgat gttgtcggcg agcttgtcga tcgactccgc gaagtacacc
     1861 gtgctgcccg cggtcaccgg catgtggatc gtcccggtct gctcggcgat gtgcgacagc
     1921 ggcaggtacg acagcgaggt gtcgccggcg acccggccgc cgccgacgtc gatcagcagc
     1981 tgcgacgtcc acgccaggtt ggcgtgcgac agcatcacgc ccttgggcgg accggtggtg
     2041 cccgaggtgt agatcagcgt cgcgaggtcc gacggctgca gcgcggcgag ccgggcgtcg
     2101 agctcggcgt cgccggcggc cgtggcctcg cccttggcgt tgaacgcctc ccacgacagg
     2161 atgccttctc cgctggccgg agccggaggt tctgtggtcg tgccgcgcat caccacgatc
     2221 cagcgcagca gcggcagctg gtcgcgccgc gcgatcacct tcgcgagctg gccggcgtcc
     2281 tcgaccagca cgaggtgcgc ctcgctgtgg tggatgatgt actggacctc gtcgggcgag
     2341 cacgtcgtgt agatcccggc ggcggagccg ccggccatca tcgcggcgtg atcgaggatc
     2401 acccactcga gccggttgaa gcccaggatc gcgaccttgc cgccgcgcgg gaatcccagc
     2461 gcgatcagcg cgcgcgccgc ggtccggacg tggtcggcga ccgtcttcca ggtcgtgggc
     2521 tgccagcgcc catcgatctt ggactgatag gcgatcgtcg acgggttggt ggccgcctgc
     2581 cggagcagac ggtgcgggat cgtatcgcgg tccgccatcg ccgcgactag ctcttgccct
     2641 tcttcttgcc ggggaagtag gtcgtcgagc gcttctggcc cttggccttg atccggccct
     2701 cggagatcag cttgcggatc ggcagcgcga ggtccttggt cgtcgtgccg agctgcttgt
     2761 tgatctgctc gatgcgcagg ccggggttgg cgctgacgaa cgacgcgaag cgctgcgaca
     2821 gcgcctcgag atcggccgac gagcgcttgg cgccgcgacc gccccgagga cggccgacgc
     2881 gcccgaggtt caccgcgagg gcctccgctg cgggcgcgct gcgaccggca cggccgccga
     2941 acgccgattc cagcgtgtcg atggccgcgc ggcgtgcgag ctcggtgatc tgtgcaacga
     3001 aaccctgcac gacacgattc atctcattct ggaagtcact cattcggttc ctcgttcgag
     3061 agtcaggata ttagatcaag cgttcggcgg cattcgagca tagaagtgct cgttgcacaa
     3121 cattgcaatg gcaaatcgcg cggtccggtc cggccgccct gcgaccgtgt gccggagcgg
     3181 caaatgctgc cgagccaaag aatccatgtg atatgaacaa tcttggcgag attgacagtc
     3241 cgcccgtgag gcccggctcg ggagcgttcc gcggcgttca ccccccggtc cggccgggta
     3301 tcccacgtct ggaacgccct ggcccccgtg cgagcagcat cggcggcaat ttctcgacga
     3361 cgatgtgcgc cggcggcacc cggaacggga gttcaataag cactgtgcgc ccggggatac
     3421 cagt
//
LOCUS       DASVOR010002011         2429 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_577820,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002011 DASVOR010000000
VERSION     DASVOR010002011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_577820"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1428)
                     /locus_tag="VF516_37280"
     CDS             complement(<1..1428)
                     /locus_tag="VF516_37280"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8113461.1"
                     /translation="MRAPSRFTIRICSVITAGLVAASSPPAQGQPRGAAITVDIPYEQ
                     FVPPNGLRVLVHTDRKAPIVAVNIWYHVGSKNEPRGRSGFAHLFEHLMFQGSENFKGE
                     FFEPFELVGATDQNGTTNSDRTNYFENVPTTALDVALWMESDRMGHLLGAIDQHLLDE
                     QRGVVQNEKRQGENQPYGRAFTMILKAIYPEDHPYHHSTIGSMNDLDAAKLDDVKNWF
                     RSWYGPNNAVLVLAGDVDLATARDKVTKYFGDIAPSANLAKLPPKLALRAAATRETAT
                     DNVPQTRIYKVWNGPQFGSVDSDRLALLSVVLGGSKSSRLDKRLTFTEKLADSVSAGM
                     LLQEIGGALFVQADVKAGVDPAKAEAAIDEEIARLVTEGPTAAEVEQARASELAGFVR
                     RVERIGGFGGKADVLAQCQVFTGNPACYRTRLANYQSATAAQLKEVAGKYLARGPFTL
                     QIDPGERKPVVEEPAVANLPPTQVAP"
     gene            1549..1815
                     /locus_tag="VF516_37285"
     CDS             1549..1815
                     /locus_tag="VF516_37285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113462.1"
                     /translation="MEYTPEMLAMLEAQRQHFASDDHIRREVEAWRDATPEERLAAVA
                     QMCAAGDYFLSQLDPETLERVLRPVPLPDDTIQILMALRQSAAE"
     gene            1812..2072
                     /locus_tag="VF516_37290"
     CDS             1812..2072
                     /locus_tag="VF516_37290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113463.1"
                     /translation="MNAFELSQHIAARLEEDGLEYAIGGALALTAWAIPRDTNDVDIS
                     VFASEPELPRVIDALERAGVMVDRVDAAKSVGRIGMFSRTRR"
     gene            2113..2244
                     /locus_tag="VF516_37295"
     CDS             2113..2244
                     /locus_tag="VF516_37295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113464.1"
                     /translation="MNTTRASNGSLQLLLRRAPTVTGIGLVVVPALATAAAATGDSS"
BASE COUNT          373 a          833 c          798 g          425 t
ORIGIN      
        1 cggcgcgacc tgggtcggcg gcaggttggc gaccgccggc tcctcgacca ccggcttgcg
       61 ctcgccggga tcgatctgca gcgtgaacgg gccgcgcgcc aggtacttgc cggcgacctc
      121 cttgagctgc gccgcggtcg cgctctggta gttcgccagc ctcgtccggt agcacgccgg
      181 gttgccggtg aacacctggc actgcgccag cacgtcggcc ttgccgccga acccgccgat
      241 ccgctcgacc cggcggacga agcccgccag ctccgacgcg cgcgcctgct cgacctcggc
      301 cgcggtcggc ccctcggtga ccagcctggc gatctcctcg tcgatcgcgg cctcggcctt
      361 cgcggggtcg acgccggcct tgacgtcggc ctggacgaac agcgccccgc cgatctcctg
      421 cagcagcatg cccgccgaga cgctgtcggc cagcttctcg gtgaacgtca gccgcttgtc
      481 gagccgcgac gacttcgagc cgccgagcac caccgacagc agcgccagtc gatcgctgtc
      541 cacgctgccg aactgcggcc cgttccacac cttgtagatc cgggtctgcg gcacgttgtc
      601 ggtcgcggtc tcgcgggtcg ccgcagcgcg cagcgcgagc ttgggcggca gcttggcgag
      661 gttcgccgac ggtgcgatgt ccccgaagta cttcgtcacc ttgtccctgg cggtcgccag
      721 atcgacgtcg ccggcgagca ccagcaccgc gttgttcggg ccataccagc tgcggaacca
      781 gttcttgacg tcgtccagct tggccgcgtc gaggtcgttc atcgagccga tcgtcgagtg
      841 gtggtacggg tgatcctcgg gatagatcgc cttcaggatc atcgtgaacg cccgcccgta
      901 gggctggttc tccccctggc gcttctcgtt ctggaccacc ccgcgctgct cgtcgagcag
      961 gtgctgatcg atcgcgccga gcaggtggcc catccggtcg gactccatcc acagcgcgac
     1021 gtcgagcgcc gtggtcggca cgttctcgaa gtagttggtg cgatccgagt tggtcgtgcc
     1081 gttctggtcg gtcgcgccga ccagctcgaa cggctcgaag aactcgccct tgaagttctc
     1141 cgagccctgg aacatcaggt gctcgaacag gtgggcgaac ccgctgcgtc cgcgcggctc
     1201 gttcttggac ccgacgtggt accagatgtt gaccgcgacg atcggcgcct tgcggtcggt
     1261 atggaccagc acgcgcagcc cgttgggcgg cacgaactgc tcgtacggga tgtcgacggt
     1321 gatcgccgct ccgcgcggct gcccctgcgc cggcggtgag ctcgccgcga cgagcccggc
     1381 ggtgatgacg ctacagatcc ggatcgtgaa tcgagatggc gcgcgcatgg ttccccctca
     1441 gcgcgcgcag catctcggtt caccgccccg acgacgagtg gaacacctgc gacagcccgc
     1501 gacgccggtg acgcacgcgg tcatcagcgg gcatggtacc ctgcgggcgt ggagtacacg
     1561 ccggagatgc tcgcgatgct cgaagcgcag cggcagcact tcgccagcga cgaccatatc
     1621 cggcgcgagg tcgaggcctg gcgagacgcc acacccgagg agcggctcgc cgccgtcgcc
     1681 cagatgtgcg ccgccggtga ctacttcctg tcgcagctcg atccggagac cctggagcgg
     1741 gtcctgcggc ctgttccgtt gcctgacgat accatccaga tcctgatggc cctgcgtcag
     1801 agcgccgcgg aatgaacgcg ttcgaactat cgcagcacat cgcggcgcga ctcgaggagg
     1861 acggcctgga gtacgcgatc ggcggtgctc tcgcgctcac tgcgtgggcg attcctcgag
     1921 acacgaacga cgtcgacatt tccgtgtttg cttcggaacc ggagctgcca cgcgtcatcg
     1981 atgcgctgga gcgagccggt gtgatggtcg accgcgtcga cgcggcgaag tcagtaggcc
     2041 ggatcggaat gttcagccgg actcgcagat gacctcccgg ctttgccagg taaccggtca
     2101 attttcagtg gcttgaacac aacacgcgcg agcaacggca gcctgcagct gctgttacgg
     2161 cgggcaccca ctgttacagg catcgggctc gtcgtcgtgc cggcgctcgc caccgccgcc
     2221 gccgcgaccg gcgacagctc gtgagtttct cgcgtgactc gctcgcgata ctcgcgggat
     2281 cactcgaccg cccaggacgc gtgggacatc gcagggaaat caccagtctg acgctttgcg
     2341 gctatccatc ggtgagtgac gaaacaaact tattgtagaa tttcaccagc tgttgtacga
     2401 ttccatcgta ggatattcgc gatgagtaa
//
LOCUS       DASVOR010002012         5879 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_400455,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002012 DASVOR010000000
VERSION     DASVOR010002012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5879
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400455"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF516_37300"
     CDS             <1..337
                     /locus_tag="VF516_37300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113465.1"
                     /translation="PPAVSRSAARPPRDKLPVITIAKAKHKADALAGLERWKARHPAV
                     VAHLAPEHVLIDTNRGRSSAWYRVRINLRDVPEAERPPPESPDPDYDWKSEYAPDDPR
                     DHDPDRRED"
     gene            482..1030
                     /locus_tag="VF516_37305"
     CDS             482..1030
                     /locus_tag="VF516_37305"
                     /inference="COORDINATES: protein motif:HMM:NF015448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HEX8113466.1"
                     /translation="MIAALCGAGATAPMASSEAVKTAVRDLLRHAGFKPSGRSKPASE
                     YLAAAFAEQRFPRINAVVDACNAVSLWSGLPISLVDADRLSGPLAIRVAPAGTSYVFN
                     PSGQMIDASGLVCLYDGEGPSGTPVKDAQRTKTHDGSRRVLSIVWGTRALPGRTAEAV
                     RWYRALVGAIDGAAIEDVTLAS"
     gene            1234..2532
                     /locus_tag="VF516_37310"
     CDS             1234..2532
                     /locus_tag="VF516_37310"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HEX8113467.1"
                     /translation="METHRDSHGATFVGILQYAPVWGVAALLGMAACAPGASSPPGDP
                     DPGSSGDPNPGPGGDPGPGPAPSSIQVTVDGLEPGWLVTTSRLTDGSAPSDTTQVSDG
                     SSIALTGTASDVFVAVVTDGTGNLVATHAMAAPCTMARSRQLRVPGEFSTIQAAVDAA
                     RPGDTVAVGPGTYTESVTLRPGVCLVGSGPARTVLDAGGAPRTLIDLTDAPGSMVAGF
                     TLRGVTMPSGCASTDPFTCSGNWYRAGIFLGGTAWRDPTQDAPPMILGNVFEDNDIGV
                     MLYWRGVAVIRNNVFVGNRSGFVANHFQSRTLIANNVFLDNTELAIGNQAAFLDIIEN
                     VIAGSQLGIRFEFVQTGFIRCNLFFDNGADSNEPRFALGRDGNLGGDPAFVDPGAHDF
                     HLRPGSPGIDAGCHGDTFDPDGTPQDIGAYGGPLAAWAQR"
     gene            2589..3218
                     /locus_tag="VF516_37315"
     CDS             2589..3218
                     /locus_tag="VF516_37315"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEX8113468.1"
                     /translation="MEDSTVCDSSQVLELRQYTLHPGQRDTLIDLCDRMFIEPQEAAG
                     MRVIGQFRDLDAADRFVWLRGFPDLASRAARLQAFYGGPVYGKNEFPRLPVREGEHAF
                     VWFSSFAGAADLERHRAELARSDRQWTIRWISSRTGAMDPPVVGGFAGDRGEFYGEDG
                     DEGRPVRVRFVWTRLAPDAARWEQAFSYDGGPWETNWVMELTRVTPRSA"
     gene            complement(3190..3468)
                     /locus_tag="VF516_37320"
     CDS             complement(3190..3468)
                     /locus_tag="VF516_37320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113469.1"
                     /translation="MTGFVHQVKYNPMLDAAFVWVGPTSADTVLCYIQITVGDDESTA
                     LVKSSMIDGLTAAMLGGRSVTLSTDDDNPSAIESAAIHPVTPSAESPA"
     gene            complement(3477..3794)
                     /locus_tag="VF516_37325"
     CDS             complement(3477..3794)
                     /locus_tag="VF516_37325"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8113470.1"
                     /translation="MPATLGRLSDELLATGVHVEVTDDPPAHFRITAGGSIDSGGPEG
                     TAVRIRGPAERIAALLGGRKHPTAVFVEGGIQIAGDVRQITALSEELPALTMERRSAA
                     GRS"
     gene            complement(4020..5105)
                     /locus_tag="VF516_37330"
     CDS             complement(4020..5105)
                     /locus_tag="VF516_37330"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEX8113471.1"
                     /translation="MTLQLPFQPPVEPMLAKASSDLPSEGTGYVYEPKWDGFRALVWK
                     SGDEVYIQSRDLKPLGRYFPELEAALRSGLPDRCVLDGEIVIATHGRLDFDALLLRIH
                     PAASRIQMLAEQSPSSYIAWDLLASGDDDLRARPQVERRAALERVLAGVAPPIHVTPA
                     TRDRSVAADWFDRFEGAGLDGVVAKPESLPYTPGKRVMLKIKHQRTADCVVGGFRWYK
                     GGKGTLVGSLLLGLYDDAGVLHHVGVTSAFTRPKRAELADKLAPLRDGAREVHPWRDW
                     AEWQDQETEQRLPGATSRWNRGKDLSWEPIRLGLVCEVSFEHLQGMRFRHAAHFKRWR
                     PDKPAIECRYDQLAETPAFELQKIFGS"
     gene            complement(5214..>5879)
                     /locus_tag="VF516_37335"
     CDS             complement(5214..>5879)
                     /locus_tag="VF516_37335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8113472.1"
                     /translation="ELFGWSTKGVPMPDGEYTMIAAPDGRTIGGYFAAPDPRTPAGWL
                     PYLQVESAADIAAKVKKLGGSVMKEPFKVGDFATMAVVADPHGAGFALWQPNKTEDSG
                     KPVAGHFCWNELAAKDARASVAFYTQIGGFTEKPMDMGGMGTYHVLESDGQGRAGIMA
                     GQPPQPHAWLPYVQVANADQTADKAKRLGATIVVPPTPVPGVGRFAIFVDPQGAPTGI
                     LQP"
BASE COUNT          801 a         2090 c         2117 g          871 t
ORIGIN      
        1 gccgccggcc gtgtcccggt ccgctgcgcg gccgccgcgc gacaagctgc cggtgatcac
       61 gatcgccaag gccaagcaca aggccgacgc gctcgccggc ctggagcgct ggaaggcgcg
      121 gcacccggcg gtggtcgctc acctggcgcc cgagcacgtg ctgatcgaca ccaaccgcgg
      181 caggtcgtcg gcgtggtacc gcgtccgcat caacctccgc gacgtgcccg aggccgagcg
      241 cccgccgccc gagtcgccgg atcccgacta cgactggaag tccgagtacg cgcccgacga
      301 cccccgcgac cacgatccgg atcgtcgcga ggactgagcc gagccgctgg tgcgaccgcg
      361 cgatcgcgca ctatcgtagg ccagggccat ccccatcgcg gtcgagcccc atccgctcct
      421 ggaggtcggc gcgttcgtca ccgcgctgcc gcgcccgctc ggcgagatcg ccgcgccgcc
      481 ggtgatcgcg gcgctgtgcg gcgccggggc cacggcgccg atggcgagca gcgaggcggt
      541 caagacggcg gtgcgcgacc tcttgcgcca cgccgggttc aagccgtcgg gccgcagcaa
      601 gccggcgtcg gagtacctcg cggccgcgtt cgccgagcag cggttcccgc ggatcaacgc
      661 cgtggtcgac gcgtgcaacg cggtgtcgct gtggtcgggg ctgccgatct cgctggtcga
      721 cgccgaccgc ctgtccgggc cgctggcgat ccgcgtggcg ccggccggca cgagctacgt
      781 gttcaatccg tcgggccaga tgatcgatgc ctccggcctc gtgtgcctgt acgacggcga
      841 gggtccgtcg ggcacgccgg tcaaggacgc ccagcgcacc aagacccacg acggctcgcg
      901 ccgcgtgctg tcgatcgtct ggggcacccg cgcgttgccc ggccgcaccg ccgaggccgt
      961 gcgctggtac cgcgcgctgg tcggcgcgat cgacggcgcc gcgatcgagg acgtcacgct
     1021 cgcgtcctag actgtcactg ccaggctttg ccggataccc gcgcgtcgcg agccgcgaca
     1081 tcgatgccat cccgactggg tacgtgttgc gatgccgcat cacctcgcgg gcgcaagacg
     1141 acgggcgatg ccggcaacgg tgaaaatgtg cgaccgccag cacaatgcgt ggacgcggcg
     1201 ccaggtccat ggcttgcttg aggggtgggc gacatggaga cgcatcgaga cagtcatggg
     1261 gcgacgttcg tgggcattct ccagtacgcg ccggtgtggg gggtggctgc gctgctcggc
     1321 atggccgcgt gcgcgccggg cgcctccagt cctcccggcg atccggaccc cgggtcgagc
     1381 ggagatccga acccaggtcc cggcggcgat cccggccccg gtcccgcgcc gtcgagcatc
     1441 caggtcaccg tcgacggtct cgagcccggc tggctcgtga ccacgagccg gctcaccgac
     1501 ggcagcgcac cctcggacac cacgcaggtc tccgacggca gctcgatcgc gctcaccggc
     1561 accgcgagcg acgtgttcgt cgcggtcgtc accgacggca cgggcaacct ggttgcgacc
     1621 cacgcgatgg ccgcgccctg tacgatggcg aggtcgcgcc agctgcgcgt gcccggcgag
     1681 ttctcgacca tccaggccgc ggtcgacgct gcccggccgg gcgataccgt cgcggtcggg
     1741 cccggcacgt acacagagtc ggtgacgctg cgcccgggag tgtgcctggt cggcagcggt
     1801 ccagcgcgca ccgtcctcga tgccgggggg gcgccgcgaa cgctgatcga cctgaccgat
     1861 gcgccgggca gcatggtcgc cggcttcacc ctgcgcggcg tgaccatgcc atcggggtgc
     1921 gccagcaccg acccgttcac ctgctccggc aactggtacc gcgcggggat cttcctcggc
     1981 ggcaccgcgt ggcgggaccc gacccaggac gcgccgccga tgatcctcgg caacgtgttc
     2041 gaggacaacg acatcggcgt gatgctgtac tggcgcggcg tcgcggtgat ccgcaacaac
     2101 gtgttcgtcg gcaaccgcag cgggttcgtc gccaaccact tccagagccg cacgctgatc
     2161 gccaacaacg tgttcctcga caacaccgag ctcgcgatcg gcaaccaggc ggccttcctc
     2221 gacatcatcg agaacgtcat cgccggatcg cagctcggca tccggttcga gttcgtccag
     2281 accgggttca tccgctgcaa cctgttcttc gacaacggcg ccgacagcaa cgagccgcgg
     2341 ttcgcgctcg gccgcgacgg caacctcggc ggcgatccgg cctttgtcga cccgggcgcg
     2401 cacgacttcc atctgcgccc cggatcgccc gggatcgatg ccgggtgcca cggcgacacg
     2461 ttcgaccccg atggcacgcc gcaggacatc ggcgcgtacg gcggcccgct ggcggcgtgg
     2521 gcccagcgat agcctccggc cgcgatacct gacacgcggt ggcaggaatg caggcgcagg
     2581 gtgcctgcat ggaagactcg accgtttgcg attccagtca ggttctcgag ctccggcagt
     2641 acacgcttca tcccggccag cgcgacacgc tgatcgatct gtgcgatcgc atgttcatcg
     2701 agccgcagga ggcggcgggg atgcgcgtga tcggccagtt ccgcgatctc gacgccgccg
     2761 accgcttcgt ctggctgcgc gggttccccg acctcgcgtc gcgcgctgcg cgcctgcagg
     2821 cgttctacgg cggccccgtg tacggcaaga acgagtttcc tcggctgccg gtgcgcgagg
     2881 gcgagcacgc gttcgtgtgg ttctcgtcgt tcgccggcgc cgccgacctc gagcggcatc
     2941 gcgccgagct cgcacggtcc gatcgccagt ggacgatccg ctggatcagc tcgcggaccg
     3001 gcgcgatgga tccgccggtc gtcggcgggt tcgcgggcga tcgcggcgag ttctacggcg
     3061 aggacggcga cgaggggcgg ccggtgcggg ttcgcttcgt gtggacccgg ctggcgcccg
     3121 acgccgcgcg ctgggagcag gcgttctcgt acgacggtgg gccgtgggag accaactggg
     3181 tgatggagct cacgcgggtg actccgcgct cggcgtgacc ggatggatcg cggcgctctc
     3241 gatcgccgac ggattgtcat cgtcggtcga cagcgtgacg ctgcgaccgc cgagcatcgc
     3301 cgcggtcagg ccgtcgatca tgctgctctt gaccagcgcg gtcgattcgt cgtcgcccac
     3361 ggtgatctgg atgtagcaca gcaccgtgtc cgcgctggtc gggccgaccc acacgaacgc
     3421 ggcgtcgagc atcgggttgt acttcacctg gtgcacgaag ccggtcatca ccgccatcac
     3481 gacctccccg cggcgctgcg ccgctccatc gtcagcgccg ggagctcctc cgacagcgcg
     3541 gtgatctgcc ggacgtcccc ggcgatctgg atcccgcctt ccacgaacac ggctgtcgga
     3601 tgcttccggc cgccgagcag cgcggcgatc cgctcggcgg ggccccggat ccggaccgcc
     3661 gtgccctcgg ggcctccgct gtcgatgctg ccgcccgcgg tgatgcggaa atgcgccggg
     3721 ggatcgtcgg tgacctcgac atggactccg gtggcgagca actcgtcgct gagccggccg
     3781 agcgtggccg gcacgagctc gtccaggctc gggcgcttcg gggtcctcgc tgccgcctgc
     3841 cgcttcgcgg ccctgccgcc acgcgatcgt gccgccatga tgactcctgt caataccgca
     3901 ctcgacacca tgacacggat cgatgggaat gcgacgggaa tgcggctgtg cgccgccgcg
     3961 acaccgagga gcgccgagcg cgatgggaca cccttgccgg tgtgcggggg gcgcgcgggt
     4021 cagctgccga agatcttctg gagctcgaac gccggggtct cggcgagctg atcgtagcgg
     4081 cactcgatcg ccggcttgtc gggccgccag cgcttgaagt gggcggcgtg gcggaaccgc
     4141 atgccctgga ggtgctcgaa cgagacctcg cagaccaggc cgaggcggat cggctcccag
     4201 gacagatcct tgccgcggtt ccaccggctg gtcgcgccgg gcaggcgctg ctcggtctcc
     4261 tgatcctgcc actcggccca gtcgcgccag gggtggacct cgcgggcgcc atcgcgcagc
     4321 ggggcgagct tgtcggcgag ctcggcgcgc ttgggccggg tgaacgccga ggtgacgccg
     4381 acgtgatgca gcacgccggc gtcgtcgtag agcccgagca gcagcgagcc gactagggtg
     4441 cccttgccgc ccttgtacca gcggaagccg ccgaccacgc agtcggccgt gcgctggtgc
     4501 ttgatcttga gcatcacgcg cttgccgggg gtgtagggca gggactcggg cttggcgacc
     4561 acgccgtcga ggccggcgcc ctcgaagcga tcgaaccagt cggcggcgac gctgcgatcg
     4621 cgggtcgccg gggtgacgtg gatcggcggc gcgacgccgg cgagcacgcg ctcgagcgcg
     4681 gcgcggcgct cgacctgcgg ccgcgcgcgc aggtcgtcgt cgccggacgc gagcaggtcc
     4741 cacgcgatgt acgacgacgg cgactgctcg gcgagcatct ggatccggct cgcggcggga
     4801 tggatgcgga gcagcagcgc gtcgaagtcg agccggccgt gggtcgcgat cacgatctcg
     4861 ccgtcgagca cgcagcggtc gggcaggccg gaccgcagcg cggcctcgag ctcggggaag
     4921 tagcggccga gcggcttgag atcgcggctc tggatgtaga cctcgtcgcc gctcttccag
     4981 accagcgcgc gaaacccgtc ccacttgggc tcgtagacgt agccggtgcc ctcgctcggc
     5041 agatcgctcg atgccttggc cagcatcggc tcgaccggag gttgaaatgg cagctgcagg
     5101 gtcacgccgg cacggtacac cgggatcggt gccgccgctg ctcgttgcct cctctcgcgc
     5161 ggcgcgccgg tctacagcgg cgcggccggc ggcgacatgt cagcgcgctc gcgtcacggc
     5221 tgcaggatgc cggtgggggc gccctgcgga tcgacgaaga tcgcgaaccg gccgacgccc
     5281 gggaccgggg tcggtgggac gacgatggtc gcgccgagcc gcttggcctt gtcggccgtc
     5341 tggtcggcgt tcgcgacctg gacgtacggc agccaggcgt gcggctgcgg cggctggccg
     5401 gccatgatgc cggcgcggcc ctgcccatcg ctctcgagga cgtggtaggt gcccatgccg
     5461 cccatgtcca tcggcttctc ggtgaagccg ccgatctggg tgtagaacgc gaccgaggcc
     5521 ctggcgtcct tcgccgccag ctcgttccag cagaagtgcc cggcgacggg cttcccggag
     5581 tcctcggtct tgttgggctg ccacagcgcg aacccggcgc cgtgcggatc ggcgaccacc
     5641 gccatggtcg cgaagtcgcc gaccttgaac ggctccttca tgaccgagcc gccgagcttc
     5701 ttgaccttgg cggcgatgtc cgcggcgctc tcgacctgca ggtagggcag ccagccggcc
     5761 ggggtcctgg gatcgggcgc ggcgaagtag ccgccgatcg tcctgccatc gggtgcggcg
     5821 atcatcgtgt actcaccgtc gggcatcggc acgcccttgg tgctccagcc gaacagctc
//
LOCUS       DASVOR010002013         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_283254,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002013 DASVOR010000000
VERSION     DASVOR010002013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283254"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VF516_37340"
     CDS             complement(<1..328)
                     /locus_tag="VF516_37340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113473.1"
                     /translation="MAELTTDRRWKTFADVVAFTLGTNVWISIVILPAIFVGALHGGP
                     RIAAAALPFIVLLLGLARRSEGVLLGLFPAAVLVPVALQPQMASSYVYGPVRFTLVAL
                     GVIAYLF"
     gene            455..1138
                     /gene="cmk"
                     /locus_tag="VF516_37345"
     CDS             455..1138
                     /gene="cmk"
                     /locus_tag="VF516_37345"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEX8113474.1"
                     /translation="MKQVVVTIDGPAGAGKSTVAKLLARRLGYRLLDTGAIYRAVALT
                     ARARGIAWSDAAACAEVARALDIRFDFVGDRNHVYVAGSDVTAAIRTPEISQGASQVS
                     AHPQVRAALLELQRRLGAGGGVVVEGRDTGTVVFPGAEAKFFLTASEFERARRRVSEL
                     AAAGTTVEYADTLREIQERDRRDASRDTAPMVAAPDAVLVDSSTQTIEDVVDSLVRRV
                     EAVRRGGAP"
     gene            1296..>1942
                     /locus_tag="VF516_37350"
     CDS             1296..>1942
                     /locus_tag="VF516_37350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX8113475.1"
                     /translation="MNNVTPESAGEEDFAALLAESFGQDSVKEGEILRGTVIAVGKDY
                     AIVDVGYKSEGQVPLEEFRQADGTIGVQPGDVVDVLLESRENDAGMCVLSKEKADRFK
                     VWDEISAACERDELIEGVITARVKGGLSVTIRGGVKAFLPGSQVDLRPVRNLDAFLNQ
                     SFKFKVIKFNKKRGNIVLSRRVLLEKERAALKESTLERLKEGQIVEGIVKNLTEYG"
BASE COUNT          344 a          648 c          689 g          261 t
ORIGIN      
        1 cgaacaggta ggcgatcacg ccgagcgcga ccagcgtgaa ccggaccgga ccgtagacgt
       61 acgacgacgc catctggggc tgcagcgcga ccggtaccag cacggcggcc gggaacagcc
      121 cgagcagcac cccctcggag cgccgggcca gcccgagcag cagcacgatg aacggcagcg
      181 cggccgccgc gatccgcggc ccgccgtgca gcgcgcccac gaagatcgcc ggcaggatga
      241 cgatcgagat ccacacgttg gtgccgagcg tgaaggccac cacgtcggcg aacgtcttcc
      301 accgcctgtc tgtggtcagc tcggccaccg ccacgaatgt acaacgcgat cgcggccggg
      361 tcgaacgaga cggcccgcgg cgatccggcc gcaaattcgt gcatgatgcc ggcatggcgc
      421 ggtcaggcac gctacgcagt ccgaggatgc cgacatgaag caggtcgtgg tcacgatcga
      481 tggacccgcc ggcgccggca agagcaccgt cgccaagctg ctcgcgcgcc ggctcggcta
      541 ccgcctgctc gacaccggcg ccatctatcg cgcggtcgcg ctcacggcgc gcgcgcgggg
      601 catcgcgtgg agcgacgccg cggcctgcgc cgaggtcgcc cgcgcgctcg acatccggtt
      661 cgacttcgtc ggcgacagga accacgtgta cgtcgccggc agcgacgtga ccgcggcgat
      721 ccgcacgccg gagatctcgc agggcgcttc gcaggtctcg gcgcatcccc aggtccgcgc
      781 cgcgctgctc gagctccagc gccggctcgg cgccgggggc ggtgtggtgg tcgaaggtcg
      841 cgacaccggg accgtggtgt tcccgggtgc ggaggcaaag ttcttcctca cggccagcga
      901 gtttgagcgg gcccggcggc gcgtctcgga gctggccgcg gcagggacca ccgtggagta
      961 cgcggatacg ctgcgggaaa tccaggagcg cgaccgccgc gatgccagcc gggataccgc
     1021 gccgatggtc gccgctccgg acgccgtgct cgtcgacagc tccacacaaa ccatcgaaga
     1081 tgttgtggat tcgctggttc gccgggtcga ggccgtccgc cgcggtggag cgccgtgatg
     1141 cgctgatgtg gcgacgcagc gcgcgaatcg gcctagccag cagcggatct catcgccatg
     1201 gttgacaaaa cgggtagtgg aaggtaccaa ctcgcccctg cacccaggtg ctgccatggc
     1261 agcaatccca gccggggtgg cttcaggaga tctgcttgaa taacgttacc ccagaaagcg
     1321 cgggcgaaga ggacttcgcg gctctgctcg cggaaagctt cggccaggac agcgtcaagg
     1381 agggcgagat cctccgcggc acggtgatcg cggtcggcaa ggactacgcg atcgtcgatg
     1441 tcggctacaa gagcgagggc caggtgccgc tggaagagtt ccggcaggcc gacggcacga
     1501 tcggcgtgca gcccggcgac gtcgtcgacg tcctgctgga gtcgcgcgag aacgacgccg
     1561 gcatgtgcgt gctctcgaag gagaaggccg atcgcttcaa ggtctgggac gagatcagcg
     1621 cggcgtgcga gcgcgacgag ctgatcgaag gcgtcatcac cgcgcgggtc aagggcggcc
     1681 tgtcggtcac gatccgcggc ggcgtcaagg cgttcctgcc cggatcgcag gtcgacctcc
     1741 ggccggtccg caacctcgac gcgttcctca accagagctt caagttcaag gtcatcaagt
     1801 tcaacaagaa gcgcggcaac atcgtgctgt cgcgccgcgt gctgctggag aaggagcgcg
     1861 ccgcgctcaa ggagtcgacg ctcgagcgcc tcaaggaggg ccagatcgtc gagggcatcg
     1921 tcaagaacct caccgagtac gg
//
LOCUS       DASVOR010002014         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_283278,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002014 DASVOR010000000
VERSION     DASVOR010002014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283278"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VF516_37355"
     CDS             complement(<1..401)
                     /locus_tag="VF516_37355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113476.1"
                     /translation="MVDRTDIDALLIGALYGELTPADEARLMAHLESHPADRTALDNL
                     TRTRAVVRDSRILAFQAEPPQSISALLLQEASRRAPRAATERSETATWFQRFVQSFMA
                     HPAMAAAATLVLVIAAAGTFYLRGADQFAQQ"
     gene            complement(621..989)
                     /locus_tag="VF516_37360"
     CDS             complement(621..989)
                     /locus_tag="VF516_37360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113477.1"
                     /translation="MRWATSALALAISIVPASVAPALADHPRRTIAACASFDQVDRGD
                     DRVAFTIHNTCSIPVDCAVSWRVVCAPDAKKRRASHPGSAKLAISEGASQSAEASAAI
                     CGDDAWVIDSVVWSCQPNKD"
     gene            1173..1979
                     /locus_tag="VF516_37365"
     CDS             1173..1979
                     /locus_tag="VF516_37365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8113478.1"
                     /translation="MPSPLDVFRDGVLAGRVALITGGGTGICRGIAAAYARLGAEVCI
                     VSRKQDVLDQTAAAIAADTGRAVLPVAADVRDPAAIGRAVQEAVERFGRLDTVVNGAA
                     GNFLAPAAGLSPNGFRTVIDIDLNGTFHTSRAAFEPLQKAGDGLILNISATLHYQGTP
                     LQIHASAAKAGVDAITRNLAVEWGAFGIRVCGIAPGPIAETEGVRRLAPGGVTPQIAA
                     AIPARRFGAIDEIAAAAVFLRSAAAGYITGHILVVDGGHCVARQALGLAP"
BASE COUNT          276 a          725 c          716 g          277 t
ORIGIN      
        1 tcctgctggg cgaactggtc ggcgccccgc aggtagaacg tgcccgcggc ggcgatgacc
       61 agcaccagcg tggccgccgc ggccatcgcc gggtgggcca tgaaggactg cacgaaccgc
      121 tggaaccagg tcgcggtctc gctgcgctcg gtcgccgccc gcggggcgcg ccgcgacgcc
      181 tcctgcaaga ggagcgcgga gatcgactgc ggcggctcgg cctggaacgc caggatccgg
      241 ctgtcgcgaa ccaccgcgcg cgtgcgcgtc aggttgtcga gcgccgtccg gtcggccggg
      301 tgcgactcga ggtgcgccat cagccgcgcc tcatcggcgg gcgtcagctc gccgtagagc
      361 gcgccgatca gcagcgcgtc gatgtcggta cggtccacca tgatcctgat cctggtcctt
      421 atactacgtg tgtcatcaac gagcgacgaa ccgacgaccc ctgacagttc gagcacttga
      481 cacgaacttc gtgccggctc gagggatcgc ttggttgctc gctggataca aacgcaggac
      541 cggggcggac gatctaccgt gcgccatggc gcggccgggc gcggtcctcc ccgcgccacc
      601 agcgctaacc tacgacaatt tcagtccttg ttgggctgac agctccacac caccgagtcg
      661 atgacccatg cgtcatcgcc gcagatcgca gccgatgcct cggcgctctg gctcgcgccc
      721 tcggagatcg cgagcttggc gctgccgggg tgcgaggcgc gccgcttctt cgcgtcgggg
      781 gcgcacacga cgcgccacga caccgcgcag tcgaccggga tgctgcacgt gttgtggatc
      841 gtgaacgcga cccggtcgtc gccgcggtcg acctgatcga agctcgcgca cgccgcgatc
      901 gtccggcgcg ggtggtcggc gagcgccggc gcgacggacg cggggacgat cgagatcgcg
      961 agggcgaggg cagacgtcgc ccatcgcacc gagcacacgg tacgcatgac agacctccgg
     1021 ccgacggcgg gcgagctcga tcgcggcgcg gaccggcgcg gcgatcgggc gccacgcgct
     1081 gccaggcagc gggcgccgtc gatgttcatg cataggacat cgcggatgcg ccgcggttcc
     1141 gcaattttgg ccggctggtg catgatcgcg cgatgccaag tccgctcgac gtgttccgcg
     1201 atggcgtgct cgctggccgt gtggcgctca tcaccggcgg tggtaccggg atctgccgcg
     1261 ggatcgccgc cgcctacgcc cggctgggcg ccgaggtctg catcgtcagc cgcaagcagg
     1321 acgtgctcga ccagaccgcg gccgcgatcg ccgcggacac cggccgcgcg gtgctgccgg
     1381 tcgccgccga cgtccgcgat ccggccgcga tcgggcgcgc ggtgcaggag gcggtcgagc
     1441 ggttcggccg gctcgacacc gtggtcaacg gcgccgccgg aaacttcctg gcgcccgccg
     1501 cggggctgtc gcccaacggg tttcgcaccg tgatcgacat cgatctcaac ggcacgttcc
     1561 acacctcgcg cgccgcgttc gagccgctgc agaaggccgg cgacgggctg atcctcaaca
     1621 tcagcgcgac cctgcactac cagggcacgc cgctgcagat ccacgcctcg gccgccaagg
     1681 ccggggtcga cgcgatcacg cgcaacctcg cggtcgagtg gggcgcgttc gggatccggg
     1741 tatgcggcat cgcgcccggc ccgatcgccg agaccgaggg cgtgcgccgg ctcgcgcccg
     1801 gcggcgtcac gccgcagatc gccgccgcga tccccgcccg gcggttcggc gcgatcgacg
     1861 agatcgccgc ggcggcggtg ttcctgcgct cggccgccgc cggctacatc accggccaca
     1921 tcctggtggt cgacggcggc cactgcgtcg cccgccaggc gctcgggctc gcgccatgac
     1981 gcgtcgccgc ggcg
//
LOCUS       DASVOR010002015         9864 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_79877,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002015 DASVOR010000000
VERSION     DASVOR010002015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79877"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VF516_37370"
     CDS             <1..732
                     /locus_tag="VF516_37370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8113479.1"
                     /translation="PQPEGFDRRFGNPDLGIEHGIHTGIGYEWKPDRLWSIDSELYYV
                     SRYDLAVFTSAVAQNPDGTFTNVNFLSEGKNHSTGVELLVKREISEHAYGWLSYTYSR
                     AFQQSHPDSAVVPTAFDQPHVLNAVASYKPGSGWEIGGRFQLASGRPDTPVIGATYDA
                     DSGRYVPIRGTARSIRTPLFLQLDARVEHDWLYERWSLGVYLDVINATNRQNVEALQY
                     DYRFRNSSPVTGFPILPTLGVKGTW"
     gene            726..1745
                     /locus_tag="VF516_37375"
     CDS             726..1745
                     /locus_tag="VF516_37375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113480.1"
                     /translation="MVNRALLTLVLALVLALGVVLGAAAAGCGRFQDPNVVVDLRTLA
                     IRADPPDQVIDVDLTQPVMPAALLAQLVPTQVCALVADPGADRRLSWSMTLCALLSND
                     RCDDNGPELLLGMGLSDDPETTVPEPTMCVTIMPDANLLALLAFVLQGDTLHGLGGVD
                     YGVVLRVGGENGNRDLDQYAAKTLAVSPRIPAARAANHNPSLDRIDVSLDNGDPPVPL
                     PLGRCADNPAPYALAPATKLRLMPIESAGAREVYTVPTLDGRSETFTESLTYPWIAGA
                     GGFSRGSTGGPRDLSGNPPPLFSDFKSPAASDLDGKDLVSLWIIQRDERLGVQWYEAC
                     VRVTP"
     gene            1917..2105
                     /locus_tag="VF516_37380"
     CDS             1917..2105
                     /locus_tag="VF516_37380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113481.1"
                     /translation="MRERPAMQLVKRYIDICFEATIMVDQIAMTALSASHLDREDILG
                     RAIELVERERMDEALDIS"
     gene            complement(2236..2394)
                     /locus_tag="VF516_37385"
     CDS             complement(2236..2394)
                     /locus_tag="VF516_37385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113482.1"
                     /translation="MTPGPGERLRQAEQLYWLARRLREAQERVLHPTWTDDEIASHVR
                     KIFLRAGT"
     gene            complement(2460..3152)
                     /locus_tag="VF516_37390"
     CDS             complement(2460..3152)
                     /locus_tag="VF516_37390"
                     /inference="COORDINATES: protein motif:HMM:NF018597.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HEX8113483.1"
                     /translation="MLAGVAIASLAVASAVASADSGSTRRARRAGAAKPTASNLPRGF
                     TWPPSRAMLDAEAECERSLDAAGVAWHRAAREGHIVDAVTIDGAVLGGITYTPVFGRG
                     PYKLDCQLALALVTIAPELAAAGVREVRFGSIYRWSQVRVGGKTKNALSRHALGLAMD
                     VVSFVDDTGREARVARDYPGGDPLLLEVERTIDASSGFRLVLTPKNDPISHKDHFHIE
                     ANPRYATQVSRR"
     gene            3160..3561
                     /locus_tag="VF516_37395"
     CDS             3160..3561
                     /locus_tag="VF516_37395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113484.1"
                     /translation="MVRRDIRRDDNRARTGRSGSRRSIRRGRAGAGLRKARVHHANDL
                     VTTLSHPYGQLRVQRASNRTPLAATYVKVYARQHGGQVAFFKDGYTDLRGWFDYASLS
                     TTELDAVERFAILVCSDHAGAAILEAGPPAR"
     gene            3576..4610
                     /locus_tag="VF516_37400"
     CDS             3576..4610
                     /locus_tag="VF516_37400"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113485.1"
                     /translation="MIGRVVGKYRLLAQLGEGGMGTVYRAEHVVLGSPAAVKILLPQF
                     TQDPVVVDRFFQEARAASAIRHAGIVEVFDFGRLPTGQAYIAMELLRGEELASFLARR
                     GRLDVSLAVQIAMQMLAALNATHLVGVIHRDLKPDNIFLVPDPGAPGAIRVKILDFGI
                     AKLTGRPLAGQPGRPRTKNMILGTPAYMAPEQCRGGTEIDARADLYAVGCLLFELLTG
                     RPPFVAGGDGEVMAMHIYEPPPRLSNLAPHLPVELDALLAKLLTKDPADRIPSAAYAL
                     ATLERAPVPPMVGEVALSGAHYAIGPAALLPGTDDEPPSEPMPRWIPVAVIAGALAIA
                     VAAAVAIGIT"
     gene            4765..5730
                     /locus_tag="VF516_37405"
     CDS             4765..5730
                     /locus_tag="VF516_37405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113486.1"
                     /translation="MAVPVTIRSLHDAGLHHPLAGILCHPQRIASKPRARRRPSAAPF
                     LPGWFPPPPGTLALGVGRQPNGYTCGTETFLGICEFLRLPVNDPDDDNLASYSAKLKT
                     SARYGTDPDDIVETAVGFLNIEARVVNRCTIDELAQITNGTQAYVDRLLDGGPITDPL
                     EIAMVTYQAYLDPDRVRGWMYPGGFRRARRTGTARLRTIRDGERVRWENDWSDGHWSA
                     VLRVVQGGDELAEIQQRLGGSRVRKGVVILGDPSNGEGLSFAPIPEFVRRWHDTDRRD
                     TPRFRQTAVVLRVPKQMLRRMRARSQRLRVPMFATVRRNAVLYVP"
     gene            complement(5782..7194)
                     /locus_tag="VF516_37410"
     CDS             complement(5782..7194)
                     /locus_tag="VF516_37410"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX8113487.1"
                     /translation="MAKLLLAISLIGCTLAPHDERPALPVPDRFPGGGGALAAADQGW
                     RKMFGDPRLQALIALALANNRDLRVAALNIEVARAQYRIQRAGLFPTVDARAAWSRLG
                     GGLVSSGPAAGSASSSTTAAPGLSIPPSFYVLGVSAAYELDLFGRVRSLTAQAREQYL
                     GTVAAHRATHLALVGQVVTQYLAERSLAEQYEIAVRTEATTRRTYELTKQLFELGQRS
                     AVELRATEAQWQNARGELPRLTRQWTQALDALVVLVGQPLPANLPPPQRLSVERMIAD
                     LAPGLPSQVLLRRPDVREAEHALRAANASIGAARAALFPDISLTGFAGTLSTALKRLF
                     DAGTGLGLISSLITQPVFHGGANLANLDLADAQKRIQIARYEQTIQTAFREVADALVA
                     RETYERELAAQAAQVAAQQARFDLTLQRYRAGVASYLEVLTAQNDLYTAQQRLIVLRA
                     DRLTNLADLYRALGGGWRED"
     gene            complement(7187..>9864)
                     /locus_tag="VF516_37415"
     CDS             complement(7187..>9864)
                     /locus_tag="VF516_37415"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8113488.1"
                     /translation="AFYSADGSMTRDDVADFVNSVVADPLGRVSGVGDVQVFGAQFAM
                     RIWLDPDALANRRLTAVDVSNAIAAQNVQVAAGQFGGVPAVRGQQLNATVSAKSRLQT
                     PAQFEQILVRVNPDGSRVVLRDVARVELASETFDVEADFNRRPAAALAIRLSPGANAL
                     DTADAVRARLAELSSTFPPSLRVGYPLDSIPFVKISIRDVVKTLVEAVGLVFLVMFLF
                     LQNFRATLIPTLAVPVVLLGTFAVLSAFGYSINTLTMFGMVLAIGLLVDDAIVVVENV
                     SRVMAEQGLPPREAACTSMDQITGALVGVALVLATVFLPTAFVGGSVGVIYRQFSITI
                     IAAITLSVLVALIFTPALCATILRPGGGSGRKRWFGWFNRGFDRTQRGYRRAVGTLVR
                     RRLPAMIVYLALAGAAVVLFRQLPTGFLPSEDQGQVFMQVTLPPGATQEATRGVLGQI
                     SDYLLDDEHEAVASVLAVAGFSFTGYGQNVGAVFVRLKDWEQRRARRLKAPAVVGRAM
                     AALSRIKSATIIAFQPPPVLELGLVAGFQLQLEDRAGAGHERLMQAGDELLARAARDP
                     ALAKVRPGGLADRPEYKLEIDEPKASAFGLALGDVNASIASTWGAAYVDDFLDKGRTK
                     RVFMQADAPFRMQPDDLGRWYVRNATGEMVPFSAFTTGRWRLGPQRLDRFNGFPTVAI
                     QGEPAPGHSSGDALTAISRLASQLPAGFGHEWYGPAYEQVQAGTGVLTLYALSLLVVF
                     LCLAALYESWSIPAAVLLVVPLGIVGAVTASLLGKQADDVYFQVGILAVIGLAAKNAI
                     LIVEFARGIVEQGTPPIDAAVQAAQLRLRPILMTSLAFVLGVLPLAISSGAGSGAQNA
                     IGIAIVGGVVTATVLAVLLVPVFFVLIAGRRRPHG"
BASE COUNT         1487 a         3628 c         3405 g         1344 t
ORIGIN      
        1 ccccagcccg agggcttcga ccgccggttc ggcaaccccg acctcggcat cgagcacggc
       61 atccacaccg gcatcggcta cgagtggaag cccgaccggc tgtggtcgat cgacagcgag
      121 ctgtactacg tcagccgcta cgacctcgcg gtgttcacct cggcggtcgc ccagaacccc
      181 gacggcacgt tcaccaacgt caacttcctc agcgagggca agaaccactc gaccggcgtc
      241 gagctcctgg tcaagcgcga gatcagcgag cacgcctacg gctggctgtc gtacacttac
      301 agccgcgcgt tccagcagag ccaccccgac agcgcggtgg tcccgaccgc gttcgatcag
      361 ccgcacgtgc tcaacgcggt cgcgagctac aagccgggca gcggctggga gatcggcggc
      421 cggttccagc tcgccagcgg ccggcccgac accccggtga tcggcgcgac ctacgacgcc
      481 gacagcggcc gctacgtgcc gatccgcggc acggcgcgct cgatccgcac cccgctgttc
      541 ctccagctcg acgcgcgcgt cgagcacgac tggctgtacg agcgctggag cctgggcgtc
      601 tacctcgacg tcatcaacgc caccaaccgg cagaacgtcg aggccttgca gtacgactac
      661 cgcttccgca actcgtcccc ggtcaccggc ttcccgatcc tgccgaccct gggagtcaag
      721 ggcacatggt gaaccgcgcg ctgctcacgc tcgttctcgc cctcgttctc gccctcggtg
      781 tcgtcctcgg cgcggccgcc gccggctgcg gccggttcca ggatcccaac gtcgtcgtcg
      841 atctgcgcac gctggcgatc cgcgccgatc cgccggacca ggtgatcgac gtcgatctca
      901 cccagccggt gatgccggcc gcgctgctcg cccagctcgt gccgacccag gtctgcgcgc
      961 tggtcgccga cccgggcgcc gaccggcgcc tctcgtggtc gatgacgctg tgcgcgctgc
     1021 tcagcaacga ccgctgcgac gacaacggcc ccgagctgct gctcggcatg ggcctctcgg
     1081 acgaccccga gaccacggtc cccgagccca ccatgtgcgt cacgatcatg ccggacgcca
     1141 acctgctcgc cctgctcgcg ttcgtgctgc agggcgacac gctgcacggc ctgggcggcg
     1201 tcgactacgg cgtcgtgctc cgcgtcggcg gcgagaacgg caaccgcgac ctcgaccagt
     1261 acgcggccaa gaccctcgcg gtctcgccgc ggatccccgc cgcgcgcgcc gccaaccaca
     1321 acccgtcgct cgaccggatc gacgtctcgc tggacaacgg cgaccccccg gtgccgctgc
     1381 cgctcggccg gtgcgccgac aaccccgcgc cctacgcgct cgccccggcg accaagctgc
     1441 gcctgatgcc gatcgagtcc gccggcgccc gcgaggtcta cacggtgccc accctcgacg
     1501 gccggtccga gaccttcacc gagagcctga cctacccgtg gatcgccggc gccggcgggt
     1561 tctcgcgcgg ctcgaccggc gggccgcgcg atctgtcggg caacccgccg ccgctgttct
     1621 ccgacttcaa gtcgccggcg gcgagcgatc tcgacggcaa ggacctcgtc tcgctgtgga
     1681 tcatccagcg cgacgagcgc ctcggcgtgc agtggtacga agcgtgcgtc cgcgtcacgc
     1741 cgtgatggcc cgacgaccga gcgcgccggg ttgcgctgtc tgaagtgctg cgctagacct
     1801 cggcgagagc tctggcccac tccgctgcga ggccgagacg ggcgatgctt gcgaaggatc
     1861 acgtactcga cgggcgcgac ggacacgcgt agcccatcga gcgtgagttc cctgcgatgc
     1921 gcgagcgccc agcgatgcag ctcgtcaaac gctatataga tatctgcttt gaagccacta
     1981 tcatggtgga tcagattgca atgaccgcgt tgtccgcgtc gcacctcgat cgcgaggaca
     2041 tcctcgggcg ggcaatagaa ctcgtcgagc gggaacgcat ggatgaagcg ctggacatct
     2101 cgtgaggcca gcgcgaccac gatatccaca tcgtgggtca agcgcggctc gccatagaga
     2161 atccccgcca tgctgcctgt cacgaagtac gggatgccga ggcgctcgag tcggacgatg
     2221 aagggctcga gctggctagg ttcccgcacg caggaagatc tttcgcacgt gcgaggcgat
     2281 ctcgtcgtcg gtccatgtcg ggtgcagcac gcgctcttgc gcttcgcgta gccgccgggc
     2341 caaccagtag agctgctcgg cttgccgcag ccgttccccg ggcccgggtg tcatggcacg
     2401 gtatcgcgct agttgttccg gtgatggccg ctccacgagg cggagggtag cacggtcgac
     2461 tatcgtcgcg agacctgggt cgcgtagcgc gggttggcct cgatgtggaa gtggtccttg
     2521 tgcgagatcg gatcgttctt cggggtcagc accaggcgaa accccgatga cgcgtcgatc
     2581 gtgcgctcga cctcgagcag cagcgggtcg ccgcccggat agtcgcgggc gacgcgggcc
     2641 tcgcggccgg tgtcgtcgac gaacgacacg acgtccatcg ccaggccgag cgcgtggcgc
     2701 gacagcgcgt tcttggtctt gccgccgacg cggacctggc tccagcggta gatgctgccg
     2761 aaccggacct cgcggacgcc ggcggcggcg agctcggggg cgatggtgac cagcgcgagc
     2821 gcgagctggc agtcgagctt gtacgggccc cggccgaaca ccggcgtgta ggtgatgccg
     2881 ccgagcacgg cgccgtcgat cgtcaccgcg tcgacgatgt ggccctcgcg ggccgcgcgg
     2941 tgccaggcga cgccggcggc gtcgagcgag cgctcgcact cggcctcggc gtcgagcatc
     3001 gcgcgcgacg gcggccaggt gaagccgcgg gggagattgc tggcggtcgg cttcgcggcg
     3061 ccggcgcggc gggcacggcg ggtcgatccg gagtcggcgg acgcgacggc ggacgcgacg
     3121 gcgaggctgg cgattgccac tcccgcgagc atggcagcga tggtccggcg cgacatccgc
     3181 cgcgacgata acagagctcg gaccgggagg tcaggatcga ggaggtcaat ccgtcgcggc
     3241 cgagccggcg ccggcctgcg caaggcgagg gtccaccatg ccaacgacct ggtaaccacg
     3301 ctgtcacatc cgtacggcca gctccgggtc cagcgcgcct cgaaccgcac cccgctcgcc
     3361 gcgacctacg tcaaggtcta tgcccgccag cacggcggcc aggtcgcttt cttcaaggac
     3421 ggttacaccg atctgcgcgg ctggttcgac tacgcctcgc tgtcgaccac cgagctcgac
     3481 gcggtcgagc ggtttgccat cctcgtgtgc tccgatcacg ccggggccgc gatcctggag
     3541 gccggtcccc ccgcccggtg atacgctgat cgcgcgtgat cggccgcgtc gtcggcaaat
     3601 accgtctgct cgcccagctc ggcgagggcg gcatgggaac ggtgtaccgc gccgagcacg
     3661 tcgtgctggg cagcccggcg gcggtcaaga tcctgctgcc gcagttcacc caggacccgg
     3721 tcgtcgtcga tcggttcttc caggaggcgc gcgccgcgag cgcgatccgc cacgccggca
     3781 tcgtcgaggt gttcgacttc ggccggctgc ccaccggcca ggcctacatc gcgatggagc
     3841 tgctccgcgg cgaggagctg gcgagcttcc tcgcccgccg cggcaggctc gacgtctcgc
     3901 tcgcggtcca gatcgccatg cagatgctcg cggcgctcaa cgcgacccac ctggtcggcg
     3961 tgatccaccg cgacctcaag cccgacaaca tcttcctcgt ccccgacccc ggggcgccgg
     4021 gcgcgatccg ggtcaagatc ctcgacttcg gcatcgccaa gctgaccggc cggccgctcg
     4081 ccggccagcc cggccggccg cggaccaaga acatgatcct cggcaccccg gcctacatgg
     4141 cgcccgagca gtgccgcggc ggcaccgaga tcgacgcgcg cgccgacctc tacgccgtcg
     4201 gctgcctgct gttcgagctg ctcaccggcc ggccgccgtt cgtcgccggc ggcgacggcg
     4261 aggtcatggc gatgcacatc tacgagccac cgccgcggct gtccaacctc gcaccccacc
     4321 tgccggtcga gctcgacgcg ctgctcgcca agctgctcac caaggacccc gccgaccgga
     4381 tcccgtcggc cgcctacgcg ctcgccactc tcgagcgtgc gccggtcccg ccgatggtcg
     4441 gcgaggtcgc cctgtcgggt gcgcactacg cgatcggccc cgccgcgctg ctgcccggca
     4501 ccgacgacga gccgccgtcc gagccgatgc cgcgctggat cccggtcgcc gtgatcgccg
     4561 gcgcgctcgc gatcgccgtc gccgccgccg tcgcgatcgg catcacgtag caccgtcccg
     4621 cacctcgatc tccgggcgcc ccacgacctg cgcgcggagc ccaccctcga catcgccgtg
     4681 tgcgccggcg acgcagcgcc gcctgccacg atctgccgcg aacgtgcagc gaggcctcgg
     4741 gtaccctgga gagatgtggc ggcgatggcg gtgccagtga cgatccgcag tctgcacgac
     4801 gcaggactcc accacccgct cgccgggatc ctgtgtcatc cgcaacgcat cgcgagcaag
     4861 ccccgcgcga ggcggcggcc ctccgcggcg ccgttcctcc cggggtggtt tccacctcca
     4921 cccggcacgc tcgcgctcgg cgtcgggcgg cagccgaacg gctacacctg cggcacggag
     4981 accttcctgg gcatctgcga gttcctgcgc ctgcccgtga acgatcccga tgacgacaac
     5041 ctggcgtcgt acagcgccaa gctcaagacc agcgcgcggt acggaaccga tccggacgac
     5101 atcgtcgaga ctgcggtcgg gttcctgaac atcgaggcgc gcgtggtcaa tcgctgcacc
     5161 atcgacgagc tggcgcagat cacgaacggg acccaggcct acgtcgaccg cctgctcgac
     5221 ggtggcccga tcaccgatcc gctcgagatc gcgatggtca cgtaccaggc gtacctcgat
     5281 cccgatcgcg ttcgcggctg gatgtacccg ggcgggttcc gtcgcgctcg ccggaccggc
     5341 acggcgcggc tgcgcacgat cagggacggc gagcgcgtgc ggtgggagaa cgactggagc
     5401 gacggtcact ggtcggccgt gctgcgcgtg gtccagggcg gagacgagct cgccgagatc
     5461 cagcagcggc tcggaggcag ccgggtccgc aagggcgtgg tgatcctcgg ggacccatcg
     5521 aacggcgagg gcctcagctt cgcgccgatt ccggaattcg tgcggcgctg gcatgacacc
     5581 gaccgtcgcg acacgccgag gtttcgacag accgccgtcg tgctccgcgt gccgaagcag
     5641 atgctgcggc ggatgcgcgc caggtcgcag cggctccgtg ttccgatgtt cgcgacggtg
     5701 cggcgcaacg ccgtgctcta cgtgccgtga gggtggccgc tcccgccggc gaacccgggt
     5761 gccggctcga tccgggtcgg ttcagtcctc gcgccagcca ccgccgagcg cgcggtacag
     5821 gtcggcgagg ttggtgagcc ggtcggcgcg cagcacgatc agccgctgct gcgcggtgta
     5881 caggtcgttc tgcgcggtca gcacctcgag gtagctcgcg acgcccgccc ggtagcgctg
     5941 caaggtcaga tcgaaccgcg cctgctgggc cgcgacctgc gcggcctgcg cggcgagctc
     6001 gcgctcgtag gtctcgcgcg cgaccagcgc gtcggcgacc tcgcgaaacg cggtctggat
     6061 cgtctgctcg tagcgcgcga tctggatccg cttctgcgcg tcggccaggt cgaggttcgc
     6121 gaggttcgcg ccgccgtgga acaccggctg cgtgatgagc gacgagatca gcccgagccc
     6181 ggtgccggcg tcgaacaggc gcttcagcgc cgtgctcaac gtgcccgcga acccggtgag
     6241 cgagatgtcc ggaaacagcg ccgcgcgcgc cgcgccgatg ctggcgttgg cggcgcgcag
     6301 cgcgtgctcg gcctcgcgca catcggggcg gcggagcagg acctgcgacg gcaggcccgg
     6361 cgcgagatcc gcgatcatcc gctcgaccga gagccgctgc ggcggcggca ggttcgcggg
     6421 cagcggttgc ccgaccagga cgaccagcgc gtcgagcgcc tgcgtccact gccgggtcag
     6481 cctcggcagc tcgccgcgcg cgttctgcca ctgcgcctcg gtcgcccgca gctcgaccgc
     6541 cgagcgctgg ccgagctcga acagctgctt cgtgagctcg taggtgcggc gcgtcgtggc
     6601 ctcggtgcgg accgcgatct cgtactgctc ggcgaggctg cgctcggcga ggtactgggt
     6661 cacgacctgg ccgaccagcg ccaggtgcgt cgcgcggtgg gccgccacgg tgccgaggta
     6721 ctgctcgcgg gcctgcgcgg tgaggctgcg cacgcggccg aacaggtcga gctcgtacgc
     6781 ggcgctgacc ccgagcacat agaacgaggg cgggatgctc aggcccggcg ccgcggtggt
     6841 ggacgacgac gcagaccccg cggccggtcc actggacacc aacccgccgc cgagccgact
     6901 ccacgccgcc cgcgcatcca ccgtcgggaa cagcccggcg cgctggatcc ggtactgcgc
     6961 gcgcgcgacc tcgatgttga gcgcggccac gcgcagatcg cggttgttgg ccagcgcgag
     7021 cgcgatcagc gcctgcaggc gcgggtcgcc gaacatcttg cgccagccct ggtcggcggc
     7081 ggcgagcgca ccaccgcccc ccggaaaccg atcgggcacc ggcagggccg ggcgctcgtc
     7141 gtgcggcgcc agcgtgcatc cgatcagcga gatggcgagc agcagcttag ccatggggcc
     7201 tccggcgacc tgcgatcagg acgaagaaca ccggcacgag caggacggcg agcaccgtcg
     7261 cggtgaccac gccgccgacg atcgcgatgc cgatcgcgtt ctgcgcaccc gagccggcgc
     7321 cgctcgagat cgcgagcggc agcacgccga gcacgaacgc gagcgaggtc atgaggatcg
     7381 gccgcagccg gagctgcgcg gcctgcaccg cggcgtcgat cggcggcgtg ccttgctcga
     7441 cgatgccccg cgcgaactcg acgatcagga tcgcgttctt cgcggccagg ccgatcaccg
     7501 cgaggatgcc gacctggaag tagacgtcgt cggcctgctt gccgagcagc gacgcggtca
     7561 ccgcaccgac gatgccgagc ggcacgacca gcagcaccgc ggccgggatc gaccagctct
     7621 cgtacagcgc ggccaggcac aggaacacca ccagcagcga cagcgcgtag agcgtgagca
     7681 cgccggtgcc cgcctggacc tgctcgtacg cgggcccgta ccactcgtgt ccgaaccctg
     7741 caggcagctg ggacgcgagc cggctgatcg cggtcagcgc gtcccccgag ctgtggccgg
     7801 gcgccggctc gccctggatc gccacggtcg gaaacccgtt gaagcggtcg agccgctgcg
     7861 ggcccagccg ccagcgcccg gtcgtgaacg ccgagaacgg caccatctcg ccggtggcgt
     7921 tgcgcacgta ccagcggccg aggtcgtccg gctgcatgcg gaacggcgca tcggcctgca
     7981 tgaacacgcg cttggtacgg cccttgtcga gaaagtcgtc gacgtacgcg gcgccccagg
     8041 tcgacgcgat cgacgcattg acgtcgccga gcgcgaggcc gaacgccgac gccttgggct
     8101 cgtcgatctc gagcttgtac tcggggcggt ccgcgagccc gccgggccgc accttcgcca
     8161 gggccggatc ccgcgcggcg cgcgcgagca gctcatcccc ggcctgcatc aggcgctcgt
     8221 gcccggcccc cgctcgatct tcgagctgga gctgaaagcc ggcgaccagg cccagctcga
     8281 gcaccggcgg cggctggaac gcgatgatgg tcgccgactt gatgcgcgac agcgcggcca
     8341 tcgcacgccc gacgaccgcg ggcgccttga gccgcctggc ccggcgctgc tcccagtcct
     8401 tgagccggac gaacaccgcg cccacgttct ggccgtaccc ggtgaacgag aatccggcga
     8461 ctgcgagcac cgacgcgacg gcctcgtgct cgtcgtcgag caggtagtcg gagatctgcc
     8521 ccaggacccc gcgggtcgcc tcctgggtcg cgcccggcgg cagcgtcacc tgcatgaaga
     8581 cctggccttg atcctcgctg ggcaagaacc ccgtgggcag ctggcggaac agcacgaccg
     8641 ccgcgccggc gagcgccagg tacacgatca tcgccggcag ccggcgccgc acgagcgtgc
     8701 cgaccgcacg ccggtagccg cgctgcgtcc ggtcgaaccc gcggttgaac cagccgaacc
     8761 agcgcttgcg cccggacccg ccccccggcc ggaggatcgt cgcgcacagc gccggcgtga
     8821 agatcagcgc gaccagcacc gacagcgtga tcgccgcgat gatcgtgatc gagaactggc
     8881 gatagatcac gccgaccgag ccgccgacga acgcggtcgg caggaacacg gtcgcgagga
     8941 ccagcgcgac gccgaccagc gccccggtga tctggtccat cgacgtgcag gccgcctcgc
     9001 gcggcggcag cccttgttcg gccatcacac gcgagacgtt ctcgaccacc acgatcgcgt
     9061 cgtcgaccaa aaggccgatc gcgagcacca tgccgaacat ggtcagcgtg ttgatgctgt
     9121 agccgaacgc ggacagcacg gcgaacgtgc cgagcagcac gaccggcacc gcgagcgtcg
     9181 ggatcagcgt cgcgcggaag ttctgcagga acaggaacat cacgaggaac accaggccga
     9241 ccgcttcgac cagggtcttg accacgtcgc ggatcgagat cttcacgaac gggatcgaat
     9301 cgagcgggta gcccacgcgc aggctgggcg gaaacgtcga cgacagctcg gcgagccggg
     9361 cgcgcacggc gtctgcggtg tcgagcgcgt tcgccccggg cgacagccgg atcgcgagcg
     9421 ccgccgccgg ccggcggttg aaatcggcct cgacatcgaa cgtctcgctg gcgagctcga
     9481 cgcgcgcgac gtcgcgcagc accacgcgcg agccgtccgg gttgacgcgc acgaggatct
     9541 gctcgaactg cgcaggcgtc tgcagccgcg acttcgcgct gaccgtcgcg ttgagctgct
     9601 gaccgcgcac cgccggcacg ccgccgaact gcccggccgc gacctggacg ttctgcgccg
     9661 cgatcgcgtt gctgacatcg accgcggtga gccgccggtt ggcgagcgcg tcgggatcga
     9721 gccagatccg catcgcgaac tgcgcgccga acacctggac gtcgccgacc cccgacaccc
     9781 ggccgagcgg atcggccacc accgagttca cgaagtccgc gacgtcgtcg cgcgtcatgc
     9841 tgccgtcggc ggagtagaac gcga
//
LOCUS       DASVOR010002016         2119 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_255635,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002016 DASVOR010000000
VERSION     DASVOR010002016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255635"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VF516_37420"
     CDS             <1..235
                     /locus_tag="VF516_37420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8113489.1"
                     /translation="EQLILGHLRAVMSGRTAVLISHRVAAIKDADQILVIDQGRIVER
                     GSHDELLAAGGLYGELYRTQLQTELGVTRKEAV"
     gene            261..>2119
                     /locus_tag="VF516_37425"
     CDS             261..>2119
                     /locus_tag="VF516_37425"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8113490.1"
                     /translation="MAAVAPAGPIGPVGPRRSGLEAEAVLGKAYDVRLIRRLAQYVRP
                     HARLLLAWVAFMLLRTSFELGQPWLVATVLEHHILGDRIDLLPFDALAYAGLVVGQNV
                     SSFFEQWFVQLAGQRTMHDLRIAIFDHVLAQRAAFFDRIPVGRLMTRMTNDIESLNEM
                     FSQGAITIVGDMVKAVAIMGVLLYYDVQLALITFATLPLLFVIVEYARRAMRTSFRQI
                     RVRLAAMNGFAQEHLFGIRVVQLLGRGQIAQREYNEINAGHRDAYLLQIRADAGMYAL
                     VEAIGYVAIGGVLWWGSQHRLLPMIGGSAAQTAAVIGIVWKFYQYIIQFFEPIRDLSA
                     KYAVMQGAMAASERIFQLLDTDERDGDNTSADVTKPEWEQPTKRQPTGPAGELAGVHF
                     SYGAEPVLRGVDVRVSRGSTIAMVGATGSGKSTVIKLLTRLYERDRGAIRIDGVDVRD
                     LALAELRRRITVVSQDVILFAGTLEDNITLGKPYARDPLETAIQRVGLDRALARRGTG
                     LETKVAERGANFSAGERQLVAFARALLRDPEILVLDEATAHVDPEAEELIEKGVAELM
                     KGRTTLVIAHRLSTIRNADLIVVMDKGQVIEQGKHDELIARGGCYAALERTFRR"
BASE COUNT          315 a          733 c          750 g          321 t
ORIGIN      
        1 cgagcagctg atcctcggtc acctgcgcgc cgtgatgtcc ggccgcaccg cggtgctgat
       61 ctcgcaccgg gtcgccgcga tcaaggacgc cgaccagatc ctggtgatcg accagggccg
      121 gatcgtcgag cgcggcagcc acgacgagct gctcgccgct ggcgggctgt acggcgagct
      181 gtaccgcacc cagctccaga ccgagctcgg ggtcacccgc aaagaggccg tgtgaccggc
      241 tgtatgatgg aggcggtgta atggcggcgg tcgcgccggc ggggccgatc ggtccggtcg
      301 ggccgcggcg ctcggggctc gaagccgagg ccgtgctcgg caaggcctac gacgtccggc
      361 tgatccgccg gctggcgcag tatgtccggc cccacgctcg gctgctgctc gcctgggtgg
      421 cgttcatgct gctccgcacc agcttcgagc tcggtcagcc ctggctggtc gcgacggtgc
      481 tggagcacca catcctgggc gaccggatcg acctcctgcc gttcgatgcg ctcgcctacg
      541 cggggctggt ggtcggccag aacgtgtcgt cgttcttcga gcagtggttc gtccagctcg
      601 ccggccagcg cacgatgcac gacctgcgga tcgcgatctt cgaccacgtg ctggcccagc
      661 gcgccgcgtt cttcgaccgc atcccggtcg gccggctgat gacccggatg accaacgaca
      721 tcgagagcct caacgagatg ttctcgcagg gcgcgatcac gatcgtcggc gacatggtca
      781 aggcggtcgc gatcatgggg gtcctgctgt actacgacgt ccagctcgcg ctgatcacgt
      841 tcgcgaccct gccgctgctg ttcgtgatcg tcgagtacgc gcggcgcgcg atgcggacgt
      901 cgttccgtca gatccgggtc cggctcgcgg cgatgaacgg gttcgcccag gagcacctgt
      961 tcggcatccg cgtggtccag ctgctcggcc gcggccagat cgcgcagcgc gagtacaacg
     1021 agatcaacgc cggccaccgc gacgcctacc tgctccagat ccgggccgac gccgggatgt
     1081 acgcgctggt cgaggcgatc ggctacgtcg cgatcggcgg cgtgctgtgg tggggttcgc
     1141 agcaccgcct gcttccgatg atcggtggca gcgcggcgca gaccgcggcg gtgatcggga
     1201 tcgtctggaa gttctaccag tacatcatcc agttcttcga gccgatccgc gatctgtcgg
     1261 ccaagtacgc cgtgatgcag ggcgcgatgg ccgcgagcga gcggatcttc cagctgctcg
     1321 acaccgacga gcgcgacggc gacaacacca gcgccgacgt gaccaagccc gagtgggagc
     1381 agccgaccaa gcgccagccc accgggccgg cgggcgagct cgccggcgtg cacttcagct
     1441 acggcgccga gccggtgctg cgcggcgtcg acgtccgggt cagtcgcggc tcgacgatcg
     1501 ccatggtcgg cgccaccgga tcgggcaagt cgaccgtgat caagctgttg acccggctgt
     1561 acgagcgcga ccgcggcgcg atccggatcg acggcgtcga cgtccgcgac ctcgcgctcg
     1621 ccgagctgcg ccgccggatc accgtggtgt cgcaggacgt gatcctgttc gccggcacgc
     1681 tcgaggacaa catcacgctc ggcaagccgt acgcccggga cccgctcgag accgcgatcc
     1741 agcgggtcgg gctcgaccgc gcgctggcgc gccgcggcac cggcctcgag accaaggtcg
     1801 ccgagcgcgg tgcgaacttc tcggccggcg agcgccagct ggtcgcgttc gcgcgcgcgc
     1861 tgctccgcga ccccgagatc ctcgtgctcg atgaggccac ggcgcacgtc gaccccgagg
     1921 ccgaggagct gatcgagaag ggcgtcgccg agctgatgaa ggggcggacc acgctggtca
     1981 tcgcccaccg gctgtccacg atccgcaacg ccgacctcat cgtggtgatg gacaagggtc
     2041 aggtcatcga gcagggcaag cacgacgagc tgatcgcccg gggtgggtgc tacgcagcgc
     2101 tggagcggac gttccgccg
//
LOCUS       DASVOR010002017         5162 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_400507,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002017 DASVOR010000000
VERSION     DASVOR010002017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5162
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400507"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="VF516_37430"
     CDS             <1..291
                     /locus_tag="VF516_37430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113491.1"
                     /translation="LAAAGPAAAPADLDAAMTEANRAYDRGDLDDAKTLASRLLARQP
                     TNVRMLRIMVSASCIDGDGAAALASFMKLPAPDQAQMRVRCARYGVSFPDKL"
     gene            392..853
                     /locus_tag="VF516_37435"
     CDS             392..853
                     /locus_tag="VF516_37435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113492.1"
                     /translation="MSSLVELLAKEPLRARVIEDCVELIESQVKQKGFILKSAYATIK
                     AIKKRFIPEVVDTMLDEWLDKLSPHFDRWSANKTSSFADYVIARGDDVAEDLLSVTDG
                     RAARTSHTTAKKMYGRMRDGAKKNVIEAIPALARMIEKHVQGAPRQPAATA"
     gene            complement(958..1965)
                     /locus_tag="VF516_37440"
     CDS             complement(958..1965)
                     /locus_tag="VF516_37440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113493.1"
                     /translation="MALSVQVVQALGVRVLDRHETELVEQAMAAVAAHSPADALVLQG
                     LIGELKATSDLLERQRPLRRPTALGGEDRDESTLIDHLCTLDGLSGDLALPLKATQSR
                     TYLLTKINFLRGFVKATSVLGDVPGSARMTHDLREELAQSIYTLLAEELFLALLRKPD
                     VSRRTKQRAADQLITVWDDAALEIDDFAPLLESAWHARNRINSAYGTLLGATETFRLV
                     REDCSPEVLEFFGREGMSADESAAFEEFLFNMTSEELATLRREMQKQHLSAASPAWAA
                     EVLARQIEELEHAHEIDPMALYRSYQRRQLAADFRLLSNAPGPRRTAEGYLMVYLLDQ
                     Q"
     gene            complement(2155..2769)
                     /locus_tag="VF516_37445"
     CDS             complement(2155..2769)
                     /locus_tag="VF516_37445"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEX8113494.1"
                     /translation="MLHQLAHWAQDIIGKFGYPGVFVLITLESTLVPIPSEVVMPFAG
                     ALAAEGKFSLPVILVINSVAAMVGSGLCYGLGVVGGRPFLVKYGKYFLVSQHEIARTE
                     AFFARHGKTTILIARFLPGIRHVISVPAGIARMSLPGFFLQTFLGATIWGGVLILLGY
                     YLGANWETFTNTLERVDLPIKIILVLALVALGIRFVVRRRRERL"
     gene            complement(2923..4200)
                     /locus_tag="VF516_37450"
     CDS             complement(2923..4200)
                     /locus_tag="VF516_37450"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8113495.1"
                     /translation="MAMAISLTPAHHRWRARVFVATWLSYVGFYFCRQPFSKAKGAIA
                     VTTHWDTRTLGDIWVAYLIAYALGQFLASSMGPRLGPRRNVLLGMAVSVAVTLAMGAA
                     PAWPVMAGLAALNGLAQATGWSGNVGTMASWFHKHERGKIMGVWSTNFTVGALVPGWV
                     IAAVLGVAPWPWAFYSGAAVLAVIWVQFYVLQRTRPEDLGLAAIEDPVAEAVRQPRDA
                     TAAPEPERLSRDAWINMLLVGGFYFFIKLIRYALWSWVPYFLKTGYGLSDSNSALFST
                     AFDLAGLPGVLVTGWLSDRYFGSRRGGVSLLMTLGAVASTGLLIAIGGASATLFIVLL
                     AAIGFFLYGPDSLLTGAGAIDIGSRRLATFAAAFISGIGSLGAVVQEFAISRQYKPGE
                     GLGVVFAMLFGSAVLAAAFCAALVWRNRRGKGI"
     gene            complement(4498..>5162)
                     /locus_tag="VF516_37455"
     CDS             complement(4498..>5162)
                     /locus_tag="VF516_37455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113496.1"
                     /translation="TATATTTAPAPAPEATGTEWDSGMFTFDMHNPKLQSGIGISVLA
                     GGGVAQFRRQAMRNTTSDIGGLWDVHATFGTHLPLALDLAYVGTATHVGGLPTGATRT
                     LIGSAAEGTVRWNVLPNYVWTPYVFGGVGYQRYDVKGGNVSLSDSGMNSSDNLLSVPA
                     GLGFVWRGNNGLVADVHGTYRYTWYQDLVLNSDARVTDNNHSNFVKLDSWEASAAIGY
                     EF"
BASE COUNT          833 a         1741 c         1852 g          736 t
ORIGIN      
        1 ctcgccgccg ccggccccgc ggccgcgccc gccgacctcg acgcggcgat gaccgaggcc
       61 aaccgggcgt acgaccgcgg cgacctcgac gacgccaaga cgctcgcgtc gcgcctgctc
      121 gcccggcagc cgaccaacgt ccggatgctc cgcatcatgg tgtcggcgtc gtgcatcgat
      181 ggtgacggcg cggcggcgct ggccagcttc atgaagctgc cggcgccgga tcaggcgcag
      241 atgcgggtcc gctgtgcgcg ctacggcgtg tcgtttcccg acaagctctg atctacgtcc
      301 cggctacgtc ccggctacgt cccggcggcg ggcgctggac ggctcgccgg gcaggcacta
      361 tggtagcgca tcgctaagca aggataggtg gatgtccagt ctggtcgagt tgctcgccaa
      421 ggagcccctt cgcgcccgcg tgatcgaaga ctgcgtcgag ctcatcgagt cgcaggtcaa
      481 gcagaagggc ttcatcctca agagcgccta cgcgacgatc aaggcgatca agaagcggtt
      541 catccccgag gtcgtcgaca ccatgctcga cgagtggctc gacaagctat cgccgcactt
      601 cgaccgctgg tcggcgaaca agacctcgtc gttcgccgac tacgtgatcg cccgcggtga
      661 cgatgtcgcc gaggacctcc tgtcggtgac cgatgggcgc gccgcccgga cgtcgcacac
      721 caccgccaag aagatgtacg gccggatgcg cgacggtgcg aagaagaacg tgatcgaggc
      781 gatccccgcc ctcgcgcgga tgatcgagaa gcacgtccag ggtgcaccca ggcagcccgc
      841 cgcgacggcg tgaacaacgc gctgcggccc ccgatcgctt cgttgtcgct gcggtcgctg
      901 cgcctggggg cgctggcgcc gtgccggcgc tgtgccggcg ctgtgccggc gctgtgatca
      961 ttgctgatcg agcaggtaga ccatgagata gccctcggcg gtgcgccgcg ggccgggcgc
     1021 gttcgacagc aggcggaagt ccgcggcgag ctggcggcgc tggtagctgc ggtacagcgc
     1081 catcggatcg atctcgtggg cgtgctcgag ctcctcgatc tggcgagcca ggacctcggc
     1141 cgcccacgcc gggctggccg ccgacaggtg ctgcttctgc atctcgcggc gcagcgtggc
     1201 cagctcctcg ctggtcatgt tgaacaggaa ctcctcgaac gccgcgctct cgtcggcgct
     1261 catcccctcg cggccgaaga actccaggac ctcggggctg cagtcctcgc ggaccagccg
     1321 gaacgtctcg gtcgcgccga gcagcgtgcc gtaggcgctg ttgatccggt tgcgggcgtg
     1381 ccaggccgac tcgagcagcg gcgcgaagtc gtcgatctcg agcgccgcgt catcccacac
     1441 ggtgatcagc tgatcggcgg cgcgctgctt ggtccggcgg ctgacgtcgg gcttgcggag
     1501 cagcgccagg aacagctcct cggcgagcag ggtgtagatc gactgggcga gctcctcgcg
     1561 caggtcgtgg gtcatccgcg ccgagccggg cacatcgccg agcaccgacg tcgccttcac
     1621 gaagccgcgg aggaagttga tcttggtgag caggtaggtg cgcgactggg tcgccttgag
     1681 cggcagtgcg aggtcgcccg acagaccgtc gagcgtgcac aggtggtcga tcagcgtgga
     1741 ctcgtcgcgg tcctcgccgc cgagcgcggt cggccggcgc agcgggcgct ggcgttcgag
     1801 caggtcggac gtggccttga gctcgccgat caggccctgg aggaccaggg cgtcggcggg
     1861 ggagtgtgcg gcgaccgccg ccatcgcctg ctccacgagc tcggtttcgt gtcggtcgag
     1921 cacacggact cccagggcct gcacaacttg gacggacagc gccaagccgg tggaccctag
     1981 cacgcccggc tgcgtcgtcg cgcgcggcac caccgtcggc gcgggcgccg tggatgcgag
     2041 cgcgggaggc tctgtgggat tgcggccggg atacgacacg gagtgttacc tagctcgggc
     2101 tccagagggc gaagatgccg gggaagatgc gcggagagcg aggggcaagg tggtttacag
     2161 ccgttcgcgc ctgcggcgta ccacgaaccg gattcctaat gccacaaggg ctagcacgag
     2221 gatgatcttg atggggaggt cgacgcgctc gagggtgttg gtgaaggtct cccagttggc
     2281 cccgaggtag tagccgagga gaatcaggac gccaccccaa atcgtcgccc ccaggaatgt
     2341 ctggaggaag aaccccggca gcgacatccg ggcgatgccg gccgggaccg agatcacgtg
     2401 ccggatcccc ggcaggaacc gcgcgatcag gatcgtcgtc ttgccatgcc tggcaaagaa
     2461 cgcctcggtc cgggcgatct cgtgctggct gacgaggaag tacttgccgt acttgaccag
     2521 gaacggtcta ccaccgacga caccgagccc gtagcacagg ccggagccga ccatcgccgc
     2581 gacgctgttg atgaccagga tcacaggtag cgagaactta ccttcggccg ccagggcccc
     2641 ggcgaatggc atcacgacct ccgacgggat cggtaccagt gtgctctcca gcgtgatcag
     2701 cacgaacacc ccgggatacc cgaattttcc gatgatgtct tgagcccagt gggcgagctg
     2761 gtgcagcacg gggagctttt acaccggcgg gccgtagaag taacgttcag caaaattgaa
     2821 cgaccacttt acctgacttc gcgctcgacg ggcacgtgat gaacacgtga tccagcggcg
     2881 catgcgcgcg ccgggcgcgg ccggcacggg ctcgggctaa cgtcagatgc ccttgccgcg
     2941 ccggttgcgc cagaccaggg cggcgcagaa cgcggccgcg agcaccgcgc tgccgaacag
     3001 catcgcgaac accacgccga ggccctcgcc gggcttgtac tggcgggaga tcgcgaactc
     3061 ctggaccacg gcgcccaggc tgccgatccc cgagatgaac gcggcggcga acgtggcgag
     3121 ccggcggctg ccgatgtcga tcgcgccggc gccggtcagc agcgagtcgg gcccgtacag
     3181 gaagaagccg atcgcggcga gcagcacgat gaacagcgtc gcgctggcgc cgccgatggc
     3241 gatgagcagc ccggtcgacg ccaccgcgcc cagcgtcatg agcagcgaca ccccgccgcg
     3301 ccggctgccg aagtagcggt ccgacagcca gccggtcacc agcacaccgg gcaggccggc
     3361 gaggtcgaac gcggtcgaga acagggcgga gttgctgtcg ctgaggccat agccggtctt
     3421 gaggaagtac ggcacccagg accacagggc gtagcggatc agcttgatga agaagtagaa
     3481 cccgccgacc aggagcatgt tgatccacgc atcgcgcgac agccgctcgg gctcgggggc
     3541 ggccgtcgcg tcgcgcggct gtctcacggc ctcggccacg ggatcctcga tcgcggccag
     3601 gccgaggtcc tcggggcgcg tgcgctgcag gacgtagaac tggacccaga tcacggcgag
     3661 cacggcggcg ccggagtaga aggcccacgg ccacggggcc acgccgagca cggccgcgat
     3721 cacccagccc gggaccagcg cgccgaccgt gaagttggtc gaccagaccc ccatgatctt
     3781 gccgcgctcg tgcttgtgga accagctggc catcgtgccg acgttgccgg accagccggt
     3841 cgcctgggcc aggccgttga gggcggccag gccggccatc accggccagg ccggcgccgc
     3901 gcccatcgcc agcgtcaccg cgaccgacac cgccatgccg agcaggacgt tgcggcgcgg
     3961 gccgagccgg ggacccatgc tcgaggccag gaactggccg agcgcgtagg cgatcaggta
     4021 cgccacccag atgtcgccga gcgtccgcgt gtcccagtgc gtggtgacgg cgatcgcgcc
     4081 cttggccttg gagaacggct ggcggcagaa gtagaacccg acgtacgaca gccaggtcgc
     4141 cacgaacacc cgggcgcgcc agcggtggtg cgcgggggtc aacgagatcg ccatcgccat
     4201 gcgccgcgag cgtatcgcgt cccggtggcg ggatggcaac gcgcaggtca ccggccggtg
     4261 atgcaccgga atgcggcacc cagcgcgcgg tcgaggcgct cgaggggccg cctcacgcag
     4321 gaactcggcc ggcgggttcg ggagctcgag ctccagcacg agctcctgga agggccgcgc
     4381 ccggggcaac tgtgactttg ccgggccggc cgtgcgcgcc cggccccccg ggggcgggcc
     4441 cgaccggaga tcgctctccc cgcgtcacgc gcgccgcccg gcggttgggc gtcgcgatca
     4501 gaactcgtag ccgatcgcgg cgctggcttc ccagctgtcc agcttgacga agttgctgtg
     4561 gttgttgtcg gtgacccgcg cgtcgctgtt gagcaccagg tcctggtacc aggtgtagcg
     4621 gtacgtgcca tggacgtcgg cgaccagccc gttgttgccg cgccacacga acccgaggcc
     4681 ggccgggacg ctcagcaggt tgtcgctcga gttcatgccg ctgtcggaca gcgacacgtt
     4741 gccccccttc acgtcgtagc gctggtaccc cacgccgccg aacacgtacg gcgtccagac
     4801 gtagttcgga agcacgttcc agcgcactgt gccctcggct gcggagccga tcagcgtgcg
     4861 ggtcgcgccg gtcggcaggc cgccgacgtg ggtcgccgtc ccgacgtagg cgagatcgag
     4921 cgccagcggg aggtgcgtgc cgaacgtcgc gtggacatcc cacaggccgc cgatgtccga
     4981 cgtcgtgttg cgcatcgcct gcctgcggaa ctgggcgacg ccgccgccgg cgagcacgct
     5041 gatcccgatg cccgactgca gcttgggatt gtgcatgtcg aaggtgaaca ttccgctgtc
     5101 ccactcggtg ccagttgcct cgggggccgg agccggggcg gtggtggtgg cggtggcggt
     5161 gg
//
LOCUS       DASVOR010002018         3672 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_547728,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002018 DASVOR010000000
VERSION     DASVOR010002018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547728"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF516_37460"
     CDS             complement(<1..442)
                     /locus_tag="VF516_37460"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8113497.1"
                     /translation="MPFTILTGWLGAGKTTALNRMLAATHGKRIAVLVNELGRISIDT
                     QLIIGRGGDVLELAGGCVCCKVDTRNDLWDGIGDIVARSSPDSVVLETTGIAEPAAIL
                     DGLVRVPDAVRDRILPAGVVCVVDAEAGGAALEARDEAREQAMAA"
     gene            complement(476..850)
                     /locus_tag="VF516_37465"
     CDS             complement(476..850)
                     /locus_tag="VF516_37465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113498.1"
                     /translation="MARVLVIDDEPDVVRLILKVLSGRGHVVQTARDGASALARVQHE
                     PPDVILLDSDLPKIDGAEVCRRIKTDAATERIPIVMITSSYIDIYDVGADGGPDAFVV
                     RPFAREVLANVVDRILFRRARA"
     gene            complement(855..1691)
                     /locus_tag="VF516_37470"
     CDS             complement(855..1691)
                     /locus_tag="VF516_37470"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEX8113499.1"
                     /translation="MSAVEPSGSSPSPLPVAVAIEKPDSPDPKDDRASSRPSRGIVID
                     PRVMFVGGAVALIGVAVFALFLWMVPGAAERELAAACRGLKPEPELKPQLCPGGQACS
                     APVPAPDFTAQDVTGKMVKLSDFRGKVVLLNFWASWCNVCKTEKPKLNDMASEMASKD
                     DFVVLALASDRNWADALGAVLDSLAPEAAASLPKNPTMAQVSAAFRQALPNGTPFNVL
                     LDPPDGDGNIGKIAASWGITAVPESALIDRQGNIRAYFGNRRDWRLPVVATCLQSLID
                     AR"
     gene            1729..2826
                     /locus_tag="VF516_37475"
     CDS             1729..2826
                     /locus_tag="VF516_37475"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8113500.1"
                     /translation="MSVRGALGTMPAEDVLEWVGRRRLSAPITFEHRGTQRSLVVESG
                     AIVWASSNRRDEQLGAILVKSGIVAERALAEALEARAETGVPLGKVLLMSGLIAEIDL
                     VEILSNKIRETVTDVVTWTEGQFDVVPRSQPPATGVNAQLPIEICLTVARRRAPRMTA
                     IMDVLGSDDATFHVPPNALPPPREDGQGMDLPRIWGMAGDRRTAADIAAALAGERYAA
                     YDALARLVEAGRLTVDRRHRERTNSAVELAAGARVRLRQGDRAGALAMASQALHQDPS
                     DAEVRKTFTQIERARVAEVAKQLLSRHRVPKRLRAPAPEHGLNATELELANRVDGRWD
                     LVSLVKSAGVREAEALLAFAHLAEVGVVELG"
     gene            2844..3626
                     /locus_tag="VF516_37480"
     CDS             2844..3626
                     /locus_tag="VF516_37480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113501.1"
                     /translation="MEAVAPTTIDRTRLLPRSGIGVDGAWLVIAPPGNPIIRLDARLL
                     DRVELIGRSPTGPLLASGLATAGVAAAALSIVPGGVFACAGLAYLAHERFSEARRRSR
                     SRDLLLALGDLEVALHVADGPEAAKGIAEQLAPYTRDAPITRPEVYEDAKRRMRAQAE
                     GKGEVSARRELEHALLVGADVVYLRGDYLQVGQVAFRISEVREYALRGANLPLTGGRL
                     LQAAMGLLVVAAEERAATGEDVALLSKRIADYEAWSGHTAGR"
BASE COUNT          506 a         1358 c         1302 g          506 t
ORIGIN      
        1 cggcggccat cgcctgctcg cgcgcctcgt cgcgggcctc cagcgcggcc ccacccgcct
       61 ccgcgtcgac cacgcagacc acgccggccg gcaggatgcg atcgcgcacc gcgtccggca
      121 cccggaccag accgtcgagg atcgcggcgg gctcggcgat gccggtggtc tcgagcacca
      181 ccgagtccgg cgacgaccgc gcgacgatgt cgccgatgcc atcccacagg tcgttcctgg
      241 tgtcgacctt gcagcacacg cagccgccgg cgagctcgag cacgtcgccg ccgcggccga
      301 tgatcagctg ggtgtcgatc gagatccggc cgagctcgtt gaccagcacg gcgatccgct
      361 tgccgtgggt cgcggccagc atccggttga gcgccgtggt cttgccggcg ccgagccagc
      421 cggtgaggat cgtgaacggc acgcgggtca tcgtctggct ccggtgaccc cggggtcacg
      481 cccgcgcgcg gcgaaacagg atccgatcga cgacattggc gagcacctcg cgggcgaacg
      541 gccggaccac gaacgcgtcg ggcccgccgt cggcgcccac gtcgtagatg tcgatgtacg
      601 acgacgtgat catcacgatc gggatccgct cggtggcggc gtcggtcttg atccggcggc
      661 acacctcggc gccgtcgatc ttcggcagat cgctgtcgag caggatcacg tcgggcggct
      721 cgtgctggac ccgggcgagc gcgctggcgc cgtcgcgcgc ggtctgcacg acgtggccgc
      781 ggcccgacag caccttcaag atcagccgga cgacatcggg ctcgtcgtcg atcaccagca
      841 cccgcgccat gggctcagcg cgcgtcgatg agggattgca ggcaggtcgc gaccaccggc
      901 agcctccagt cgcgccggtt gccgaagtac gcgcggatgt tgccctggcg atcgatcagc
      961 gcgctctcgg gaaccgccgt gatgccccac gacgccgcga tcttgccgat gttcccgtcg
     1021 ccgtcgggcg gatcgagcag cacgttgaac ggtgtgccgt tgggcagcgc ctggcggaac
     1081 gccgcgctga cctgcgccat cgtcgggttc ttggggaggg acgcggccgc ctccggtgcg
     1141 agcgaatcca gcaccgcgcc gagtgcgtcg gcccagttgc ggtccgaggc cagcgcgagc
     1201 acgacgaagt cgtccttgct cgccatctcg ctcgccatgt cgttgagctt gggcttctcg
     1261 gtcttgcaca cgttgcacca cgacgcccag aagttgagca gcacgacctt gccgcggaag
     1321 tcggacagct tgaccatctt gcccgtgacg tcctgggcgg tgaagtcggg cgctggcacc
     1381 ggcgcgctgc acgcctggcc gcccgggcac agctggggct tgagctcggg ctcgggcttg
     1441 agcccgcgac acgccgccgc gagctcgcgc tccgccgcgc ccggcaccat ccacaggaac
     1501 agcgcgaaca ccgcgacgcc gatcagcgcg accgcgccgc cgacgaacat cacgcgcggg
     1561 tcgatcacga taccgcgcga cgggcgcgat gacgccctgt catctttcgg atccggcgaa
     1621 tccggcttct cgatcgcgac agcgacgggc aacggcgacg ggctcgaccc cgatggctcg
     1681 acggcggaca tgccaacaac atagccgatt ctccggtatg ctcaagggat gtcggtgcgc
     1741 ggagcgctcg gtaccatgcc cgcggaggac gtgctggagt gggtcggccg ccgccggctg
     1801 tcggccccga tcacgttcga gcaccgcggc acccagcgca gcctggtcgt cgagtccggc
     1861 gcgatcgtgt gggcgagctc gaaccgccgc gacgagcagc tcggcgcgat cctcgtgaag
     1921 agcggcatcg tcgccgagcg cgcgctcgcc gaggcgctcg aggcgcgggc cgagaccggg
     1981 gtgccgctcg gcaaggtgct gttgatgtcg gggctgatcg ccgagatcga tctggtcgag
     2041 atcctctcca acaagatccg cgagacggtc accgacgtgg tgacctggac cgagggtcag
     2101 ttcgatgtcg ttccgcgatc tcaaccgccg gcgaccggcg tgaatgccca gctcccgatc
     2161 gagatctgcc tcacggtggc gcgccgccgc gccccccgga tgaccgcgat catggacgtc
     2221 ctcggcagcg atgacgcgac cttccacgtc ccgcccaacg cgctcccgcc cccgcgggag
     2281 gatggccagg ggatggatct gccacggatc tggggcatgg cgggcgatcg tcgcaccgcc
     2341 gccgacatcg ccgccgcgct ggccggcgag cgctacgcgg cctacgacgc gctcgcccgg
     2401 ctggtcgagg ccggccggct cacggtcgac cgccgccacc gcgagcgcac caactccgcg
     2461 gtcgagctcg ccgccggggc gcgcgtccgt ttgcggcagg gcgaccgggc cggcgccctg
     2521 gcgatggcga gccaggcgct ccatcaggat cccagcgacg ccgaagtccg caagacgttc
     2581 acgcagatcg agcgcgcgcg ggtcgccgag gtcgccaagc agctgctgtc gcgccatcgt
     2641 gtcccgaagc ggcttcgtgc cccagctccc gagcacggcc tcaacgcgac cgagctggag
     2701 cttgccaacc gggtcgacgg ccgctgggac ctggtgtccc tggtcaagtc cgccggggtg
     2761 cgcgaggcgg aggccctgct cgcgttcgcg cacctcgcgg aggtcggcgt cgtcgagcta
     2821 ggctaggctt gaaccgggaa gccatggaag ccgtcgcccc gaccacgatc gaccgcacgc
     2881 ggctcctgcc gcgcagcggt atcggcgtcg acggcgcctg gctggtgatc gcgccgcccg
     2941 gcaacccgat catccggctc gacgcccggc tgctcgaccg cgtcgagctg atcggccggt
     3001 cgccgaccgg gccgctgctc gcatcgggcc tggcgaccgc gggcgtcgcc gccgccgcgc
     3061 tctcgatcgt cccgggcggc gtgttcgcgt gcgccggcct cgcctacctg gcgcacgagc
     3121 ggttcagcga ggcccgccgc cgatcgcgca gccgcgatct cctgctcgcg ctcggcgatc
     3181 tcgaggtcgc gctccacgtc gccgatgggc ccgaggccgc caagggcatc gccgagcagc
     3241 tcgcgccgta cacgcgcgac gccccgatca cccggcccga ggtctacgag gacgccaagc
     3301 gccggatgcg cgcccaggcc gagggcaagg gcgaggtctc ggcccgccgc gagctcgagc
     3361 acgcgctgct ggtcggcgcc gacgtcgtct acctgcgggg cgactacctc caggtcggcc
     3421 aggtcgcgtt ccggatctcg gaggtccgcg agtacgcgct gcgcggcgcc aacctcccgc
     3481 tcaccggcgg ccggctgctc caggcggcga tgggcctgct ggtggtcgcc gccgaggagc
     3541 gcgccgccac cggcgaggac gtcgcgctgc tgtcgaagcg gatcgccgac tacgaggcct
     3601 ggtcgggcca caccgccggt cgctgacgtt cctcgcgcag ccagttcgtc gcgcgatccc
     3661 cggggtgcag cc
//
LOCUS       DASVOR010002019         7850 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_429808,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002019 DASVOR010000000
VERSION     DASVOR010002019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7850
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429808"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..740
                     /locus_tag="VF516_37485"
     CDS             120..740
                     /locus_tag="VF516_37485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113502.1"
                     /translation="MIRSLRTAESLYQSFRQSSRQSSPHAADGDLSPPITLWMKVLEN
                     YVHAWLGPRLANLQREPAVLFDYVDRVIGAGWPGYQRWLEPRWRDPAEVGGARVELPL
                     RAVPNAVRELADHRRKRLDSPLSVTEWARMLLLFAVDHPAGFKNLFKLGPASGSRDRI
                     VALAHRLHTLAAVRNLVTHRASAGAATLGAFRASYYATFEDLVALA"
     gene            complement(746..1378)
                     /locus_tag="VF516_37490"
     CDS             complement(746..1378)
                     /locus_tag="VF516_37490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113503.1"
                     /translation="MRLLGVLVVLVGCHGAGSVAPDASTEPDVPPRSQGMTVTWNARP
                     SVPGMITDKIIVTDAVFQLEHLQLVSDAGGTTHTRLQLSWSSQGTPPDEEFPGAPTAR
                     YQQILLDMRSDVHPPFSYAYQIEGMWQDDGNSRPFRIADPTILEVPIACDVTLPANGS
                     VTVPVRLDLRNALNGVDFKSLQMKDGVLVPDVSQLADLRDALTHAFVLDN"
     gene            1492..3204
                     /locus_tag="VF516_37495"
     CDS             1492..3204
                     /locus_tag="VF516_37495"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8113504.1"
                     /translation="MGNEPQTLAGATELAAPPAPRAFLVITIDDGGSRVIDLPEGVDV
                     SFGRSRGATITLDSDKVSRMHARVRRTGDVIDVEDLGSRNGTRVNGDKIDGPRRLTTG
                     DEINVGPILAVLGVTSGLRRTSTIADVTTGETRLAAEVDRSVRYHRPVTVALLRIASD
                     TILDQIERAIRPMDLIAEDAGDDYLVILPELSREEGSAAVQRLLDFARAANVQATAAT
                     ALCPDDGTTVETLIGVLRAGLRTGRVPRAPAPKGTPVVFDPAMQRVYALVERIADTSM
                     TVLILGETGVGKELVCEAIHQRSSRRDRPLIKLNCAALPESLLESELFGYERGAFTGA
                     DKRKVGFFEAADGGTLFLDEIGDMPLPLQAKLLRVLERKVITRVGGTSEVATDARLIA
                     ATHRNLDNDVRAGRFRQDLLFRIGGFTIAVPPLRDRPSEIIPLAEHFVRTAAAEQARI
                     PPILSDDARDALAGYDWPGNVRELRNAVERALVLATDTITATDLPERLHDASHRVRPP
                     VASGADVRGQLAEVERAAIVAALDAESGNQTRAARRLGLSRRALIYKMEKYGLKPPPQ
                     RTEG"
     gene            complement(3319..3909)
                     /locus_tag="VF516_37500"
     CDS             complement(3319..3909)
                     /locus_tag="VF516_37500"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8113505.1"
                     /translation="MSTTKPSPRAPTLADLDALPPGIVGEIIEGVLYTMTKPRMRHQH
                     ATLGIGADLRSPFGLGRGGPGGWWIIPEPGIELPNTPEISPDVAGWRRERMPQMPGDE
                     PIRLVPDCVCEILSPTTRRHDQSVKMPYYAKVGVPFLWLVDIDARVLTAHRLESSDWR
                     IIGTYRDETEARIAPFDAVPLNVPDWWPPAAPPSST"
     gene            complement(3925..4206)
                     /locus_tag="VF516_37505"
     CDS             complement(3925..4206)
                     /locus_tag="VF516_37505"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX8113506.1"
                     /translation="MAKTTLDLPADLLRRVKARAAEQGRSMESLINEALENALRQAEP
                     PAAGWRAVFGRAKPGSTRDVDRFVKELRRVDPRDWGRQRTATAAKTPQC"
     gene            complement(4253..5836)
                     /locus_tag="VF516_37510"
     CDS             complement(4253..5836)
                     /locus_tag="VF516_37510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX8113507.1"
                     /translation="MLVVPQLARAEHPRPLPHPIGQPRQPVASSPVGDSTPVSGWTPL
                     TNQPTFLVDGAANPILLTDGSVLVQDAGFPDWWRLTPDQSGSYINGTWTQVASLPATY
                     SPLYHSSAVLPDGRLIIEGGEDLLSVDQTQFIASWTSQGAIFDPLANTWTPVAPPPFF
                     TGFGPFPQTIGDAQSVVLADGTYMQANCCSDGKESALLDPRTLTWTPTGAGKFDLFDE
                     EGWTLLPSGKVLTSDTFVGVPYDPSGTSSEIYDPATGAWSSAGSTVAQLWDSAATCGG
                     ERFASFEVGPAVLRPDGTVFSTGANACGAAHTAIYDSRTGTWTAGPDFPDGLGIADGP
                     AALEPNGKVLMMASPGVFNSPSTFLEWDGTALSEIPGPPNAPFDSSFFGSMLVLPTGQ
                     ILLCDFSNDIEIFTPTATPHALEAAERPLVAFTPVILQAGGSYEAFGFRFNGVSQGAA
                     YGDDFQAATNYPLVRIRNLATNHVFYSRTHDHSSMAVASNRLVSTHFDVPAGQEHGLS
                     VLEVVANGVASPPVFVIMR"
     gene            complement(6160..7635)
                     /locus_tag="VF516_37515"
     CDS             complement(6160..7635)
                     /locus_tag="VF516_37515"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX8113508.1"
                     /translation="MALAGPPRLARTVRLAAAGAIALAACSDSTVSLAPVIDLPITMD
                     SDATAAPLDSITLSVAHAGSGADLVSHMFQSGQALELPGAPFGDDLVVHMTGYVGESI
                     VGYGRTCTLNIAQGASPPAPHLFFSRITRFATIDAMPVERISGLGIAYQGGALLLGGA
                     ASDGTAVPEVERFDPATGKITSVGPVTARTHPVDALLGLSPPRVIVLGGASGMDGARF
                     VEVVDAQGVEHQDNGDMARVDLTATSLPDGRVAVIGGRSPSGGAPLGDIDIVAQQSDS
                     SLVVRKIGASLAIPRSRHTATLLGDDVGAAVLIAGGLDATGMPVATAELFKPLSEELS
                     NPTLDPKGFHAPMVVPRSGHTAMLMPDGSVLIIGGFDALDQPVATLELFSVDGGFVMA
                     GMLPVGAGLVDFAATTLPDGRILITGGRQVRNGPAVTAAYIARLNSIIGAVDVVATDH
                     LAVARAGHQAVTLCDGTVLISGGTTVPLPAERYNPPPDGRR"
     gene            complement(7626..>7850)
                     /locus_tag="VF516_37520"
     CDS             complement(7626..>7850)
                     /locus_tag="VF516_37520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113509.1"
                     /translation="DRRSVAITGDLTALAAAVNDLLAPAPTSHWYESRWAWAAGAAAI
                     AAAVLIPVTAAVTQNTTPSTWTVKPVYKWP"
BASE COUNT         1134 a         2776 c         2818 g         1122 t
ORIGIN      
        1 ggacgatccg cgcgtgctcg gcgcggcgtc caacgcgctg atcgccgcgg cgcccgaccc
       61 ggccgcgctc ggtgcgcggc gccgcggtgc ggatgacccg gctcgccggg ttcgacacga
      121 tgatccgcag cctgcgcacc gccgagtcgc tctatcaatc gttccggcag tcctcgcggc
      181 aatcgtcccc gcacgccgcc gacggcgacc tgtcgccgcc gatcacgctg tggatgaagg
      241 tgctggagaa ctacgtccac gcctggctcg gcccgcggct ggcgaacctg cagcgcgagc
      301 cggcggtgct gttcgactac gtcgatcgcg tgatcggcgc cggctggccg ggctaccagc
      361 gctggctgga gccgcggtgg cgcgatcctg ccgaggtcgg cggcgcccgg gtcgagctcc
      421 cgctgcgcgc ggtccccaac gcggtgcgcg agctcgccga ccaccgccgc aagcggctcg
      481 actcgccgct gtcggtgacc gagtgggcgc gcatgctgct gctgttcgcg gtcgatcacc
      541 cggccgggtt caagaacctg ttcaagctcg gccccgcgtc cggctcgcgc gatcggatcg
      601 tcgcgctcgc ccaccggctc cacacgctgg ccgccgtgcg caacctcgtc acccaccgcg
      661 ccagcgcggg cgccgcgacg ctcggtgcgt tccgcgccag ctactacgcg acgttcgagg
      721 acctggtcgc gctcgcctga ccgcgtcagt tgtcgagcac gaacgcgtgg gtcagcgcgt
      781 cgcgcaggtc cgcgagctgc gacacgtccg gcaccagcac gccgtccttc atctgcaggc
      841 tcttgaagtc gaccccgttg agcgcgttgc gcagatcgag ccgcaccggg accgtcaccg
      901 acccgttggc cggcagcgtg acgtcgcacg cgatcggcac ctcgaggatc gtcggatcgg
      961 cgatccggaa cggccgggag ttcccgtcgt cctgccacat gccctcgatc tggtaggcgt
     1021 aggagaacgg cgggtggacg tccgagcgca tgtcgagcag gatctgctgg tagcgcgcgg
     1081 tcggcgcgcc cggaaactcc tcgtcgggcg gcgtcccctg gctgctccag gacagctgga
     1141 gccgggtgtg cgtggtgccg ccggcgtcgc tgaccagctg gaggtgctcg agctggaaca
     1201 ccgcgtcagt cacgatgatc ttgtcggtga tcatgccggg cacgctcggg cgcgcgttcc
     1261 acgtcacggt catgccctgc gaacgcggcg gcacgtcggg ctcggtcgac gcatccggcg
     1321 cgaccgagcc agctccgtgg caacccacga gcaccaccag gaccccgagc aagcgcatgg
     1381 cgcaatacgt gctgcaacgg tagtaccacg atctttctcg tcgcaacgcc gtgatttcct
     1441 gccttcacag gtgcgcacaa tgcgacggct gtgcacgatt cgtacaaagc tatgggcaac
     1501 gaaccccaga ccctcgccgg cgcgaccgag ctcgccgcac ctcctgcgcc ccgcgcgttc
     1561 ctcgtcatca cgatcgacga cggtggctcc cgtgtgatcg atcttccaga aggggttgac
     1621 gtcagcttcg gccgctcgcg cggagcgacg atcacactcg actccgacaa ggtgtcgcgc
     1681 atgcacgcac gtgtgcgacg taccggcgat gtgatcgacg tcgaagatct cggcagccgc
     1741 aacggcaccc gggtcaacgg cgacaagatc gacggcccgc gccggctgac caccggcgac
     1801 gagatcaacg tcggtccgat cctcgccgtg ctcggcgtca cctccggcct gcggcgcacc
     1861 agcacgatcg ccgacgtcac caccggcgag acccggctcg ccgccgaggt cgatcgctcg
     1921 gtgcgctatc accggccggt caccgtcgcg ctgctccgga tcgccagcga cacgatcctc
     1981 gaccagatcg agcgcgcgat ccggccgatg gacctgatcg ccgaggacgc cggcgacgac
     2041 tacctcgtga tcctgcccga gctctcgcgc gaggaaggca gcgcggcggt ccagcgcctg
     2101 ctcgacttcg cgcgcgccgc caacgtccag gcgaccgcgg cgaccgcgct gtgccccgac
     2161 gacggcacca cggtcgagac cctgatcggc gtgctgcgcg ccggcctgcg caccggccgg
     2221 gtgccgcgcg ccccggcgcc caagggcacg ccggtcgtgt tcgatcccgc gatgcagcgg
     2281 gtctacgcgc tggtcgagcg gatcgccgac acctcgatga ccgtgctgat cctcggcgag
     2341 accggggtcg gcaaggagct ggtgtgcgag gcgatccacc agcgctcgag ccgccgcgac
     2401 cggccgctga tcaagctcaa ctgcgcggcg ctgcccgaga gcctgctcga gagcgagctg
     2461 ttcggctacg agcgcggcgc gttcaccggc gccgacaagc gcaaggtcgg gttcttcgag
     2521 gccgccgacg gcggcacgct gttcctcgac gagatcggcg acatgccgct gccgctccag
     2581 gccaagctgc tgcgcgtcct cgagcgcaag gtcatcaccc gggtcggcgg caccagcgag
     2641 gtcgcgaccg acgcccgcct gatcgccgcg acccaccgca acctcgacaa cgacgtccgc
     2701 gccggccggt tccgccagga cctgctgttc cggatcggcg gcttcacgat cgcggtgccg
     2761 ccgctgcgcg accgcccatc cgagatcatc ccgctcgccg agcacttcgt gcgcaccgcc
     2821 gccgccgagc aggcccgcat cccaccgatc ctgtccgacg acgcgcgcga cgcgctcgcc
     2881 ggctacgact ggccgggcaa cgtccgcgag ctgcgcaacg ccgtcgagcg cgccctcgtg
     2941 ctcgccaccg acacgatcac cgccaccgac ctccccgagc gcctccacga cgcctcgcac
     3001 cgggtccgcc cgccggtcgc ttccggcgcc gacgtccgcg gccagctcgc cgaggtcgag
     3061 cgcgccgcga tcgtcgccgc gctcgatgcc gagtccggca accagacccg cgccgcccgc
     3121 cggctcggcc tgtcgcgccg cgcgctgatc tacaagatgg agaagtacgg cctcaagccg
     3181 ccgccccagc gcaccgaggg ctgacgacag cccggttgcc gatcggctgc gcgatctgct
     3241 ggacttattg cttcggagcc gcgatggggt tctacttcgg gcgtttcacg aagtagcagc
     3301 gcagcgtagt gtcgatggtt acgtcgagct cggcggcgcg gccggcggcc accagtcggg
     3361 cacgttgagc gggaccgcgt cgaacggtgc gatccgcgcc tcggtctcgt ccctgtacgt
     3421 gccgatgatg cgccagtcgg aggactccag ccggtgcgcg gtcagtacgc gtgcatcgat
     3481 atcgacgagc cacaggaacg gcacgccgac cttggcgtag tagggcatct tcacggactg
     3541 gtcgtggcgg cgcgtcgttg gcgagaggat ctcgcagacg caatcaggca ccaggcggat
     3601 cggctcgtca cccggcatct gaggcatacg ctcgcggcgc cagccggcca cgtcgggcga
     3661 gatctcgggc gtgttgggga gctcgatccc tggttcgggg atgatccacc agccaccggg
     3721 ccccccgcgg ccgagtccaa aaggagagcg gaggtcggct ccgatgccca gggtggcgtg
     3781 ctggtgccgc atgcgcggct tggtcatcgt gtagagcacg ccctcgatga tttcgccgac
     3841 gatgcccggg ggcagggcgt cgaggtcagc gagcgtcgga gcccttgggc ttggcttggt
     3901 cgtgctcatg tcctacctgg gaggttagca ctgcggagtc ttggccgcgg tggcggttcg
     3961 ttgtcgtccc cagtccctgg ggtccacgcg ccgcagctcc ttcacgaagc ggtccacgtc
     4021 gcgcgtggag cccggcttcg cgcggccgaa cacggcgcgc cagcccgccg caggaggctc
     4081 ggcttgacgg agcgcgtttt ccagcgcctc gttgatcagg ctctccatcg agcggccctg
     4141 ctccgcggcg cgggccttga cgcggcggag caggtcagcg gggagatcca gcgtcgtctt
     4201 ggccacggac gcatattacc caacatatgg ttcgcggccg atcgcggcgg ggtcagcgca
     4261 tgatgacgaa caccggcggc gaggcgacgc cgttggcgac gacctcgagc acgctgaggc
     4321 cgtgctcctg gcccgccggg acgtcgaagt gggtcgagac caggcggttg gaggcgacgg
     4381 ccatgctgct gtgatcgtgc gtccggctgt agaacacgtg gttggtggcc aggttcctga
     4441 tgcgcacgag cgggtagttg gtcgcggcct ggaagtcgtc gccgtaggcg gcgccctggg
     4501 acacgccgtt gaaccggaac ccgaacgcct cgtaagagcc gccggcctgc aggatcaccg
     4561 gggtgaacgc gacgagcggg cgctcggccg cctcgagcgc gtgcggcgtg gcggtcggcg
     4621 tgaagatctc gatgtcgttc gagaagtcgc acagcaggat ctggccggtc ggcaggacca
     4681 gcatgctgcc gaagaacgag gagtcgaacg gcgcgttggg cgggccgggg atctcggaca
     4741 gcgcggtgcc gtcccactcg aggaacgtcg acggggagtt gaacacgccc gggctcgcca
     4801 tcatcaggac cttgccgttg ggctcgagcg ccgccgggcc gtcggcgatc cccagcccgt
     4861 cggggaagtc cgggcccgcg gtccaggtcc cggtcctcga gtcgtagatc gcggtgtgcg
     4921 ccgcgccgca cgcgttggcg ccggtcgaga acaccgtgcc gtcgggcctg agcacggccg
     4981 ggccgacctc gaacgacgcg aaccgctcgc cgccgcacgt cgcggccgag tcccagagct
     5041 gcgcgaccgt gctgccggcc gacgaccagg cgccggtcgc cgggtcgtag atctccgacg
     5101 aggtgccgct gggatcgtag ggcacaccga cgaacgtatc gctggtcagg accttgccgc
     5161 tgggcagcag cgtccagccc tcctcgtcga acaggtcgaa cttgccggcg ccggtcgggg
     5221 tccaggtcag ggtcctgggg tcgagcagcg cggactcctt gccatcgcta cagcagttgg
     5281 cttgcatgta cgtcccgtcg gcgagcacca cggactgcgc gtcgccgatg gtctgcggga
     5341 acggcccgaa gccggtgaag aacggcggcg gcgcgaccgg cgtccaggtg ttggccagcg
     5401 ggtcgaagat cgcaccctgc gaggtccagc tcgcgatgaa ctgcgtctga tcgacgctga
     5461 gcaggtcctc gccgccctcg atgatcagcc ggccgtcggg cagcaccgcg gacgagtgat
     5521 acagcgggct gtaggtcgcc ggcagcgagg cgacctgggt ccaggtcccg ttgatgtagc
     5581 tgccgctctg atcgggcgtc agccgccacc agtcggggaa cccggcgtcc tggaccagga
     5641 cgctgccgtc ggtgagcagg atcgggttgg ccgcgccgtc gaccaggaag gtcggctggt
     5701 tggtcagcgg cgtccacccg ctgaccggcg tgctgtcgcc gaccggggag ctcgccaccg
     5761 gctggcgggg ctgaccgatc ggatgaggga gcggccgcgg atgttcagct cgggcgagct
     5821 ggggcaccac gagcatcagt gcggaacaga gcgaggcagt cagattcctg agatgcatac
     5881 acgtcctcca cggtcgaccg taaatgagag ttactgacat acacacgtgc gtcccccata
     5941 tccaccgaca cgcggtgaac acacgcaccg cccgaggatc ggtcatacga ccccaggtcg
     6001 cagaccggga atggatcgtt gccgcgcatt gcctgctccc ggccgggcag cacctgcgac
     6061 cgccgggtgt cgcgccgtga ccatgttgtc gcgccgtgac gctgtgtcgc gaaggccgca
     6121 ggacgcgtca ccgggctgtg cgcgggcgcg cggtggggct cagcgacggc cgtcgggcgg
     6181 cgggttgtag cgctcggccg ggagcggcac ggtggtgccg ccgctgatca gcacggtgcc
     6241 gtcgcacagc gtgaccgcct ggtggccggc gcgcgcgacc gcgaggtgat cggtcgcgac
     6301 gacgtcgacc gcgccgatga tcgagttgag ccgggcgata taggcggcgg tgaccgccgg
     6361 gccgttgcgg acctgccggc cgccggtgat caggatccgg ccgtcgggca gcgtggtcgc
     6421 cgcgaagtcg accaggccgg cgccaacggg cagcatgccg gccatgacga acccgccgtc
     6481 gaccgagaac agctccagcg tggcgaccgg ctggtcgagc gcgtcgaacc caccgatgat
     6541 cagcacgctg ccgtcgggca tcagcatggc ggtgtgcccg ctgcgcggca cgaccatcgg
     6601 ggcgtggaac cccttcgggt cgagcgtcgg gttggacagc tcctcgctga gcggcttgaa
     6661 cagctcggcg gtcgcgaccg gcatcccggt ggcgtcgagg ccgccggcga tcagcacggc
     6721 cgcgccgaca tcgtcgccca agagggtcgc ggtgtggcgg ctgcgcggga tcgcgaggct
     6781 cgcgccgatc ttgcggacca cgagcgacga gtccgactgc tgggcgacga tgtcgatgtc
     6841 gccgagcggc gcgccaccgg acggcgagcg gccgccgatc acggccacgc ggccgtcggg
     6901 cagcgacgtc gcggtgagat cgacgcgcgc catgtcgccg ttgtcctggt gctcgacgcc
     6961 ctgcgcatcg accacctcga cgaaccgcgc gccatccatg cccgaggcgc cgccgaggac
     7021 gatcacgcgc ggcggggaca gaccgagcag cgcgtcgacc gggtgggtgc gtgccgtcac
     7081 cgggccgacc gaggtgatct tgccggtcgc cggatcgaac cgctcgacct ccggcaccgc
     7141 cgtgccatcg ctggcggcgc cgccgagcag cagcgcaccg ccctggtagg cgatccccag
     7201 gccgctgatc cgctccaccg gcatggcgtc gatcgtcgcg aaccgggtga tccgcgagaa
     7261 gaacaggtgc ggggccggcg gcgatgcgcc ctgcgcgatg ttcagcgtgc aggtccggcc
     7321 gtacccgacg atgctctcgc cgacgtagcc ggtcatgtgc acgacgaggt cgtcgccgaa
     7381 cggcgcgccc ggcagctcca gggcctggcc gctctggaac atgtgcgata ccaggtcggc
     7441 gccgctgccg gcgtgcgcga cggacagcgt gatggagtcg agcggcgcgg cggtcgcgtc
     7501 gctgtccatg gtgatcggga ggtcgatcac cggcgcgagc gagaccgtgc tgtcgctgca
     7561 cgccgcgagc gcgatcgccc cggcggcggc gagccggacc gtccgggcga gccggggcgg
     7621 gccggctagg gccacttgta gaccggtttc accgtccacg tcgacggagt ggtgttctgc
     7681 gtcacggcgg cggtgaccgg gatcagaacc gcggcggcga tcgccgcggc gccggccgcc
     7741 caggcccagc gcgattcgta ccagtggctg gtcggagccg gcgcgagcag gtcgttcacc
     7801 gcggcggcca gcgcggtcag gtcgccggtg atcgcgacgc tgcggcgatc
//
LOCUS       DASVOR010002020         4567 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_167285,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002020 DASVOR010000000
VERSION     DASVOR010002020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4567)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4567)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4567
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167285"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1450)
                     /locus_tag="VF516_37525"
     CDS             complement(95..1450)
                     /locus_tag="VF516_37525"
                     /inference="COORDINATES: protein motif:HMM:NF016994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HEX8113510.1"
                     /translation="MRPERLPVPGTMNLLHFLRPQPHLASSVVAAILATAICASCAAT
                     GAGAAATPATGSHAAVQPSGSHATATAATPLDRVVRDVCNKHVVLLGEDGHHGGGGTL
                     AAKTALVSRLIDECGFSAVVFEASLAEFVDLNRAFAAGKASPAYVADAIGGLWSVAGE
                     SDPLISALFERALRKHVVLAGLDGQIGSTNLYAQTGLAGELASYLADPRRSACQAELQ
                     RFARWQYDDASPFDDATRVRLRGCLTEIETAVSGRARSTPADEVAAMAIAWQGAIEVS
                     DPALSRSAKFNARDRAMYQALQWHVARLPRDSKIIVWCATVHAAKSLRGVPGLAEFVP
                     LGSYVNRDFGDAAAAIGISALGGSYGRGKAPATELVPAPPDSLEARAFTGDGSSDAGY
                     LGRADLAQYGPDTAHPLDYSKPMAAPWHEVIDGLLVLREEHPLKVVHDPKPRRASPTA
                     Q"
     gene            2232..2705
                     /locus_tag="VF516_37530"
     CDS             2232..2705
                     /locus_tag="VF516_37530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113511.1"
                     /translation="MNHVTGRLFVLSLVATGLLAFSARVRADSDQSVTRPLDVTVSCG
                     TDTLHITGEIHRLLSIRKDDAGGLHILVHSNYQGVSGTNQDGVVYHASHVDSENLNIP
                     AGGSREAEVREDFHLVGQGDAPEFTLREFFHFTFDANGNMTSFQDHSRESECGEA"
     gene            2716..4086
                     /locus_tag="VF516_37535"
     CDS             2716..4086
                     /locus_tag="VF516_37535"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX8113512.1"
                     /translation="MTTMRLALVLLLATGVAPALAAPSQPLDQQLAAVLAGHGFTGRI
                     ESTLEARLGRHIDQKRADLGRLLWFDSLIALNDDNSCSGCHSPTAGFGDTQPIAIGIN
                     NNGIVGPHRAGPRNQRRAPMVVNTAFFPTLMWNSRFASLSGDPFDNSAGLKFPPPEGL
                     GLSYLPQLLVAQAFIPPTERTEVAGFAFPGDNFAIRDEVLRRLNGSPAYRKQFGEVFP
                     DVKAGAPITFDDFGLVIAEFEFSLVFADAPIDRFARGERNALTDSQKRGALVFFGKGR
                     CVSCHAVSGQSNEMFSDFQEHVIGVPQVAPSFGNVTFDGPGANEDFGLEQVTFDPNDR
                     YRFRTSPIRNVALQPTFMHNGAFTRLDDAIRHHLDVQASATSYRAAGLPPDLQGPTGP
                     IEPVLARLDPLLATPILLTDEEFAQLVDFVRDGLLDPRAQPEHLKVLIPQALPSGRPT
                     LQFEFP"
     gene            complement(4146..>4567)
                     /locus_tag="VF516_37540"
     CDS             complement(4146..>4567)
                     /locus_tag="VF516_37540"
                     /inference="COORDINATES: protein motif:HMM:NF024584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SPASM domain-containing protein"
                     /protein_id="HEX8113513.1"
                     /translation="KQLLRPSTPTFNELHHFNGCLNRKISIDENGQVRNCPAMGRSFG
                     HHQSVTLSEIVAGPSFQSAWRARKDEIKVCQDCQYRYACTDCRAFLEDPDSEDSKPLK
                     CGYDPYTDSWTDWRGRPGAAATWETYRKRRHLPILKR"
BASE COUNT          773 a         1567 c         1403 g          824 t
ORIGIN      
        1 gcgcgcggag ctcgaacgga cccagaagtt tccacgcgcc gatcatgggg cttacgcctg
       61 ccccggagct cggacgacgc ggcaaggatc gcggtcactg tgcggtcggg ctggcgcggc
      121 gcggctttgg atcgtggacg actttcagtg ggtgctcctc gcgcagcacg agcagcccat
      181 cgatgacctc gtgccacggc gcagccatgg gcttgctgta gtcgagtggg tgcgccgtgt
      241 cgggaccgta ctgcgccagg tcggctcgcc cgaggtatcc tgcgtcgctc gagccatcgc
      301 cggtaaacgc gcgcgcttcg agcgaatccg gcggtgccgg gacgagctcg gtcgccggcg
      361 ccttgccgcg cccgtagctg cctcccagtg cggagatgcc gatcgcggcg gcagcgtctc
      421 cgaagtcgcg attcacgtac gaaccgagcg gcacgaactc ggcgagcccg ggcacgccgc
      481 gcaggctctt ggccgcatgg acggtcgcac accagacgat gatcttgctg tcccgcggca
      541 ggcgagcgac gtgccactgc agcgcctgat acatcgcccg gtcgcgcgcg ttgaatttgg
      601 cgctgcgcga caaggcgggg tcggatacct cgatcgcgcc ctgccacgcg atcgccatcg
      661 ccgccacctc gtccgcaggc gtcgatcgcg cccgccccga gaccgcggtc tcgatctcgg
      721 tcaggcatcc ccgcaaccgc acgcgcgtgg cgtcgtcgaa cggcgacgca tcgtcgtatt
      781 gccagcgagc gaatcgctgg agctcggcct gacaggccga gcggcgaggg tcggcgaggt
      841 aactggccag ctcgccggcg agcccggtct gtgcgtacag gttggtcgag ccgatctgtc
      901 catcgagtcc ggcgaggacc acgtgcttgc gcaatgcgcg ctcgaacaac gccgagatca
      961 ggggatccga ttctccggcc accgaccaca gcccaccgat cgcgtcggcg acgtaggccg
     1021 gggacgcctt gcccgcggcg aacgcgcggt tcaggtcgac gaactcggcc aggctggctt
     1081 cgaacaccac cgccgagaag ccacactcgt cgatcagccg gctcaccagg gccgtcttgg
     1141 ccgccagggt gccgccgcca ccatgatggc cgtcctcgcc gagcagcacg acgtgcttgt
     1201 tgcacacgtc gcgaacgacg cgatcgagtg gagttgcggc ggtggcagtc gcgtgcgagc
     1261 cggatggctg gacggcggcg tgggagccgg tcgcgggcgt cgccgcggcg cccgcaccgg
     1321 ttgccgcgca gctcgcacag atcgccgtgg cgaggattgc cgccacgacg ctcgacgcca
     1381 ggtggggctg agggcgtagg aagtgcaaga ggttcatcgt gccgggaacc gggagacgtt
     1441 cggggcgcat ttcattgaca tcctacagct cgagcacctg aacgccactc cggtccgggt
     1501 ctcgctcgcc gatttcaccg agatcgagct cggccggtcc acacaaccta cgcgattctg
     1561 tttgactgac ctgagctgtc accgcatgac ggctgcgcgg cgagcgcatc gctcggcagc
     1621 agcgtccgcc gggcgtagcg cttccactgg gccgcgatcg caaggaccgt cgcgccacga
     1681 aatctcacat ccttttatat cttcccaccc cgatccaatc agatcgttat cggaaacctg
     1741 acttgcatga cggaaaccgg tatccaccgt ttgatttcaa caatccgtcc gccgcattgg
     1801 ctcagcggtc ggaccatcta ccgtaaactc ccgccgctgc cttcacggtg atcggaacta
     1861 cgcctaccta ctttggacga atcagtgggt gtatgcgacg cctcacgatg cggtgcccgt
     1921 cccccccccc ggatgaacgg tgttcggtgc cgaggtcgac ggacactgag gcgagggatc
     1981 gacatcgtct acccgagcac gcaaccatcg gcggcaggat cacggccgag actgtcggtt
     2041 atcaacaccc accatgccat ctaacaagcg cggcgggacg gtccgctcct catcgagtcc
     2101 ccgacaaaca aggcggtgac ctactgttca agtgatcaga ataatccata agcacttaaa
     2161 taaaattctt gactgctcgt attggagctc gatacagttc atccccttca ctgatccata
     2221 taagggaatc tatgaatcac gtcacgggcc gtcttttcgt cttatcattg gtcgccactg
     2281 gcctgctcgc tttctccgcg cgcgtgcggg cggattccga ccagtccgtc acccgtcctc
     2341 tcgacgtgac cgtctcctgt ggcaccgata ccttgcatat aaccggcgag atccacagac
     2401 tgttgagtat tcgcaaagac gatgccggcg gccttcacat cctcgtccat agtaactatc
     2461 agggggtgag cggcaccaat caggacggtg tcgtctacca cgcttcgcat gtcgactctg
     2521 agaacttgaa cattccggcc ggcggctcgc gcgaggccga agtccgagag gacttccacc
     2581 tggtgggaca gggcgacgcg cccgagttca cgctccgaga attctttcac ttcacgttcg
     2641 acgcgaatgg caacatgacc tcgttccagg accactctcg cgagagcgag tgtggcgagg
     2701 cctgatccca tcaccatgac caccatgcgc cttgccctgg tgctccttct cgccaccggc
     2761 gtggcccccg cgctcgcggc cccgagtcag ccgctcgacc agcagctcgc cgccgtgctt
     2821 gccggccacg gcttcaccgg ccggatcgag tcgaccctcg aggcccgcct cggccgtcat
     2881 atcgaccaga agcgggccga cctgggccgg ttgctctggt tcgatagcct catcgccctg
     2941 aacgacgaca atagctgcag cggctgccac tcacccactg ccggctttgg cgacacccag
     3001 ccgatcgcga tcggtatcaa caataacggc atcgtaggtc cacatcgcgc cggcccgcgc
     3061 aatcagcggc gtgcgccgat ggtcgtcaac accgccttct tccccacgct gatgtggaac
     3121 tcgcgcttcg cctcgctctc gggcgaccca ttcgacaata gcgccggcct gaagtttccc
     3181 ccgcccgagg ggctggggct gtcctatctg cctcagctgc tggtcgccca ggccttcatt
     3241 ccgccgaccg agcgaacgga ggtcgcgggc ttcgccttcc ccggcgataa cttcgccatc
     3301 cgcgacgagg tgctacggcg cctcaacggg agccccgcct accgcaagca gtttggcgag
     3361 gtgttccccg acgtgaaggc gggcgcgccc atcacgttcg atgacttcgg gctggtgatc
     3421 gcggagttcg agttcagtct ggtatttgcg gatgccccca tcgacaggtt cgcccgtggc
     3481 gagcggaacg cgctcaccga tagccagaag cggggtgcgc tggtgttctt cggcaagggc
     3541 cgttgcgtct cctgccacgc cgtctcgggc cagtcgaacg agatgttcag cgacttccag
     3601 gagcatgtga tcggcgtgcc gcaggtcgcc ccctcattcg gcaatgtcac attcgacggg
     3661 ccaggggcca acgaggactt cggcctggaa caggtgacgt tcgacccgaa tgaccgctac
     3721 aggttccgca cctcgccgat ccggaacgtc gcactccaac cgaccttcat gcacaacggc
     3781 gccttcaccc gcctcgacga tgccatccgc caccacctcg acgtccaggc ttctgccacg
     3841 agctaccgcg ccgcgggtct accgccggac ctgcaggggc cgaccgggcc gatcgaaccg
     3901 gtgctcgcgc gcctcgatcc gctccttgcg acgcccatcc tacttaccga cgaggagttt
     3961 gcccagctcg tcgacttcgt ccgcgacggc ctcctcgatc cccgggcgca gcccgagcac
     4021 ctcaaggtgc tgatccccca ggcgttaccg agtggtcgcc cgacgctgca gttcgagttt
     4081 ccctgatcag ggtcgatccg cgctccgcgc caagcgactg gcatcgaggt ggcgggacgt
     4141 gctcgctaac gtttgaggat ggggagatga cgccgtttcc ggtaggtctc ccacgtggcg
     4201 gccgcccccg gtcgccctcg ccagtcggtc caggaatccg tgtagggatc gtacccgcac
     4261 ttgagcggct tgctgtcctc gctgtcgggg tcttcgagga atgcgcggca gtccgtgcaa
     4321 gcataacgat actggcagtc ctgacagacc ttgatctcgt ctttcctcgc tctccaggcg
     4381 ctctgaaagc tcggcccggc gacgatctcc gagagagtga cggactggtg gtggccaaat
     4441 gatcttccca tcgcagggca attccgcacc tgcccgttct cgtcgatcga gatcttccgg
     4501 ttcaagcatc cgttgaaatg gtgaagctcg ttgaacgtcg gtgtcgatgg tcgaaggagc
     4561 tgcttct
//
LOCUS       DASVOR010002021         5494 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_489145,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002021 DASVOR010000000
VERSION     DASVOR010002021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5494
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489145"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(568..891)
                     /locus_tag="VF516_37545"
     CDS             complement(568..891)
                     /locus_tag="VF516_37545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113514.1"
                     /translation="MSKHRSTQGRVSNEPQNINFEAGTALENLRAEVVDIEALARATE
                     AAAEALPAPTTDRQRIVYGRIQALATKTSEQASASLRFANAQVTALTTQLEAHRKAAA
                     HGSHG"
     gene            complement(1511..2032)
                     /locus_tag="VF516_37550"
     CDS             complement(1511..2032)
                     /locus_tag="VF516_37550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113515.1"
                     /translation="MAGGIGLWSLRTRTSAHVCSSFVAARLRIHRDWLGTSRALIAPP
                     AFFQPSRSNQVWGRRTTRGSYVSRIGHIHGWPSSSFQSGNEIPLEKGLVHLAPPQAPH
                     LGHDPHHHVQRVIRLGRRPRRVPFSGTCSPGLPPSTAALATAPKQILLHVPLPLALRR
                     HGLFMIIAEWTVN"
     gene            2368..>5494
                     /locus_tag="VF516_37555"
     CDS             2368..>5494
                     /locus_tag="VF516_37555"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HEX8113516.1"
                     /translation="MAGRPTIAKREPRPGLEPGRGNSRRPSRITRIAATPCRRHPAAN
                     HISEKAIEQAQRIAQELSAIVDTRHLLAAYLVLPHYHDDDFQQLGVERIAWAEGFSRA
                     MMPRYPKEESFWIGFPSAVMQSQRGAPQPESAPRLVAEAPADPRDFDWFVMEPHVREA
                     METAFALAGSDMVRPSHVLRAVLMGTASDMPFSSSASFQRFSQIVRLPDPGARPSGPK
                     SSLVARRLDGALRRHLEFAQQPLHKEGASTTVWGRDMVTAVLLCGPDAAALIEEAGAT
                     IEDVRDQWYAYVVSDASHRTRDDWDAWWRYAGIPLPGPVRAGFATETDVGDDKLGIAG
                     EARAIARLILDRQVEAPLSIGLLGDWGCGKSFFIEQVKKQIAELKREARPELYQNPIE
                     IEFNAWHASDSNLWASLVTHIFDEIWEKVSPTGNEEPEAARRRLLKQIEEARGAVHEA
                     EAQVEYGRIALERAEQDLARKQADLAWDRVVKDEAQDRLKALSEAAGWHEPLATINAV
                     EAAVHTLAGSSNRLRRALVALLERPFRRIAVPTIALAVVAVILWLVLGTQPGLAAQLA
                     RWLGSIASAIGALIAPLALANRKVAVLANQLGRIRDKYDAKVKDLPEAERKRLTTAHR
                     ELDSAEKSVEAAKARLADLLSQQAALDPRRRLGAFLQERVQSTVYRSQQGIISPVYQD
                     FEQLSRYMKELRAEQAKNKALDDGNIRAFDRIVIYVDDLDRCRPAHVVAMLEAVHLLL
                     ALDLFIVIVAVDSRWLTRALEVHYKDLLDTADAADGMSASTPQSYLEKIFQITYALRP
                     MDPEYFRSYVEFLADAPAEAIPPAPPVPAEDVPVSAAPRPAPAGAPSTAPTDGTALGS
                     GPSLPTLEPLAPPAPPVRTVRRGRPVIINAQEQELISRLLPLVPTPRIAKRLVNVYRV
                     IKAGTELDEQGQFEHDRCVPCLLMLAILFGRPLIAEELLRSLHEGTAPFDEPGAQLID
                     AVARRAVAKPETAAEWKRLHAALQQVRVTATVERCAREPAEIARYSLVTGHNWHTWTE
                     RPAALSPGPARR"
BASE COUNT          929 a         1860 c         1876 g          829 t
ORIGIN      
        1 gagcccccca cgtgggtgcc agcccttgcc agtctccacc gaggtcgcgg acgccccggc
       61 cgccgagcac cccggcccgg cgccggccac ctcggagtag cggagcgctc ggtggctgcg
      121 gatcaccacc gcaccgacgg ctgaaccatc acggcggcga ccgtccaaga acacaagcgc
      181 cggcggcacg agccacgacg ccacgagcca cgacggcgaa acgactgatt aggaaggacc
      241 tcgggagatc gcgagggtgg cttgtgacca gccggagcag gtcgaccagg ctgacctcga
      301 ccgcctgctc acaacccggt gccctgcgcc aactctccct tccctttttt tatttgattt
      361 gaggggagat ttcagaagaa gcacccgcca atcgcgacct cgatcctacc tccgcagtgt
      421 ccggatcctg tgccggtctc cgcctgcgct ccttgcgagc gctcggacca cgagcccccc
      481 acgtgggtgc cagcccttgc cagtctccac cgaggtcggc gcccctgtca cgacgggcgc
      541 ttcgtgccat gccgtgcgga cggttcctta tccgtgcgat ccgtgcgcgg ctgccttccg
      601 gtgcgcctcc agctgcgtgg tgagcgcggt gacctgggcg ttcgcgaagc ggagcgatgc
      661 gctcgcctgc tccgacgtct tggtggcgag cgcctggatc cgaccgtaca cgatgcgctg
      721 gcgatccgtc gtgggcgcag ggagcgcttc ggcagcagcc tccgtggcgc gggcgagcgc
      781 ctcgatgtcc acgacctccg cgcgaaggtt ctccagcgcg gtgccagcct cgaagttgat
      841 gttctggggt tcgttcgata ccctgccctg ggtgctgcga tgcttcgaca ttgcggctcc
      901 ttggcccgtc ggtgctccta caccggcggg ccgcttgtaa ctgccttgcg cctgcggcgg
      961 cgctgcctcc gatccaaaaa gaaatatata ctctgctacg gagcccgggc tcgagcgtca
     1021 ggactgtggg gaacggcgga aggactgtga gccgcgcccg cgtcgatcga gtccgctgca
     1081 gcgcgggtca tcaggccgac ccgttctcca caggccgccc tcatgctcag caggacgtac
     1141 ggtcgagggc cggttcgtag gtccacgcag cgcctcgagg acgggtccgg atcgtcagcg
     1201 cgctgggtac ggtgaatcca ggcgcttgcc gcaagacatc gcagcaggcg agtgcgtcgc
     1261 tccgcttcgc gaacgtcccg ggggccgctg caccggcgaa tccacaacgg ccgcactcga
     1321 tccgatcagg atccggaccg aacacctcac cactcacggc acgcgttcgc gtcgcacccg
     1381 atcagaagcc cgctatgccc tgatacatcg gtcaccgatc acgcatcatt gtcgccaaac
     1441 agtgaccttt cggcgcagca agcagagcga cctaaactta cggtcgtgaa tagcaattac
     1501 atccgcccaa tcagttgaca gtccactccg cgatgatcat gaagagtcca tggcggcgaa
     1561 gcgcgagcgg aagcggcacg tgcagcagga tctgtttcgg cgcggtggca agcgccgcgg
     1621 tgctgggcgg aagcccaggg gagcacgtgc cggagaacgg cacgcggcgc ggccggcgtc
     1681 caagccgtat cacgcgctgc acgtggtgat gcgggtcgtg cccgaggtgg ggagcctgcg
     1741 gcggcgcaag atgtacaaga cccttttcga gcgggatctc gtttcccgac tggaaggagc
     1801 tggacggcca gccgtggatg tggccgatcc gcgagacgta cgatcccctg gtggttcggc
     1861 gaccccagac ctggttgctc cgagagggct ggaagaaggc cggaggcgcg atcagcgccc
     1921 gcgacgtgcc gagccagtcg cggtgaatgc gaaggcgggc ggcaacgaag gagctacata
     1981 cgtgtgcgct cgtgcgagtc cgtaaggacc agaggccgat gccgccagcc acgcctcaca
     2041 cgatcaggtg tccgcgatgc gccgtgacga gccttgcagt cgaccgcagg cagcgacacc
     2101 cgtgctgatg ccaacgcgaa agctaacccg gtcgagatcc cttgaggccg aggcaccgct
     2161 gtgggacgat ggcatgatgg tcatgcaacc ggtggcggtc gagatacgtg tacgtgacga
     2221 ggggggcgag acccagacga tcgaggtagg tacagcgctc gcatcagcga tccagcgcgc
     2281 ggcaatggcg accagcagat gggtccctcc actgtccttc gagtcattgc tcgtgggaat
     2341 gctcgcgggc aacgacgacg ttgcagcatg gctggacggc caactatcgc gaagcgcgag
     2401 cctcgaccgg gtcttgaacc aggtcgaggt aactcgaggc gacctagccg aattacgcgc
     2461 atcgccgcca cgccctgtcg aagacacccc gctgcgaacc acatcagcga aaaagcaata
     2521 gagcaggccc agcggatcgc gcaggagctt tccgcgatag tcgatactcg ccacttgctg
     2581 gctgcctacc tcgtactacc tcactatcac gacgacgatt tccagcagct cggggtcgag
     2641 cggattgcgt gggcggaggg cttctcgcgc gcgatgatgc cccgctaccc gaaggaggag
     2701 tcgttctgga ttgggttccc gagtgcggtg atgcagtcgc agcggggagc accgcagccc
     2761 gagtcagcac ccaggctggt tgcggaagct ccggcggatc cgcgcgactt cgactggttc
     2821 gtgatggagc cgcacgtacg agaggcgatg gagaccgcgt tcgcgctggc aggcagcgac
     2881 atggtgaggc cctcccatgt actccgggcg gtgctgatgg gcacggcttc cgatatgccg
     2941 ttttcgtcgt ccgcatcctt ccagcgcttt tcacagatcg ttcggttgcc ggatcccggt
     3001 gctcgcccgt ctggacccaa gtcgtcgctg gttgcccggc ggctcgatgg ggcgctgcgc
     3061 cgccacctag agttcgcgca gcaaccactc cacaaggagg gagcctcgac cacggtgtgg
     3121 ggtcgcgaca tggtcaccgc ggtcctgctg tgcgggccgg acgcggcggc cctgatcgag
     3181 gaagcgggtg cgacgatcga ggacgtgcgc gatcagtggt acgcctacgt cgtgtccgat
     3241 gcgtcccacc gaacacggga cgactgggac gcatggtggc gatatgccgg catcccgctc
     3301 ccgggtccag tgcgcgcagg atttgcgacc gagaccgacg tcggggatga caagctgggg
     3361 atcgccgggg aggcacgcgc gatcgcccga ctgatcctgg atcgtcaggt cgaggcgccg
     3421 ctgtcgatcg ggttgctcgg cgactggggc tgcgggaaat cgttcttcat cgagcaggtc
     3481 aagaagcaga tcgcagagct caagcgcgag gcgcggcccg agctgtacca gaatccgatc
     3541 gagatcgagt tcaacgcctg gcacgcgtcc gactccaacc tgtgggcgag cctggtgact
     3601 cacatcttcg acgagatctg ggagaaggtg tcgccgaccg gcaacgagga gcccgaggcg
     3661 gcgcggcgcc ggctgctcaa gcagatcgag gaggcccgag gcgcggtgca cgaagccgag
     3721 gcgcaggtcg agtacggccg gatcgcgctg gagcgcgccg agcaggacct ggcgcgcaag
     3781 caagccgacc tggcgtggga ccgagtcgtg aaggacgagg cgcaggaccg gctgaaggcg
     3841 ctctccgagg ccgccggctg gcatgagccg ctcgcaacga tcaacgcggt cgaggccgcg
     3901 gtccacacgc tggccgggtc cagcaaccgc ctacggcgcg cgctcgtcgc gctgctggag
     3961 cggccgttcc ggcggatcgc ggtgccgacc atcgcactgg cggtcgtcgc agtgatcctg
     4021 tggctggtgc tcggcacgca gccggggctc gccgcgcagc tcgcacggtg gctcggctcg
     4081 atcgccagcg cgatcggcgc cctcatcgcg ccgctggcgc tcgccaaccg caaggtcgcc
     4141 gtgctggcca accagctcgg ccggatccgc gacaagtacg acgccaaggt aaaagatctg
     4201 cccgaggccg agcgcaagcg cctgaccacg gcgcaccggg agctcgatag cgccgagaag
     4261 agcgtcgagg ctgcgaaagc ccggctcgcc gacctgctca gccagcaggc cgcgctcgat
     4321 ccgcggcggc ggctcggcgc cttcctgcag gagcgcgtgc agtcgacggt gtaccgctcg
     4381 cagcagggga tcatctcgcc ggtctaccag gacttcgagc agctgtcccg ctacatgaag
     4441 gagctgcggg ccgagcaggc gaagaacaag gcgctcgacg acggcaacat ccgcgcgttc
     4501 gaccgcatcg tgatctacgt cgacgatctc gatcgctgtc ggcccgcgca tgtcgtcgcc
     4561 atgctggagg cggtccacct gctgctcgcg ctcgatctgt tcatcgtgat cgtcgcggtg
     4621 gactcgcgct ggctgacccg cgcactggaa gtccactaca aggacctgct ggataccgcc
     4681 gacgcggcgg atgggatgag cgcgtcgacg ccgcagagct acctggagaa gatcttccag
     4741 atcacctacg ccctgcggcc gatggatccc gagtacttcc gcagctacgt cgagttcctg
     4801 gccgacgcgc cggccgaggc cataccaccc gcgccacccg tgccggccga ggatgtaccg
     4861 gtgtccgccg cgccgaggcc cgcgccagcc ggggcgccgt cgaccgcgcc caccgacggc
     4921 accgcactgg gatcgggacc gtcactgccg acactggagc ccctggcgcc gccggcaccg
     4981 ccggtgcgga ccgtgcggcg cgggcgcccc gtgatcatca acgcccagga gcaggagctg
     5041 atctcacgcc tgttgccgct ggtccccacg ccgcggatcg ccaagcgcct ggtcaacgtc
     5101 taccgggtaa tcaaggccgg caccgagctc gacgagcagg gccagttcga gcacgaccgc
     5161 tgcgtcccct gcctgctcat gctcgcgatc ctgtttggcc ggccactgat cgccgaggag
     5221 ctcctgcgct cgctccacga gggcaccgcg ccgttcgacg agcccggcgc gcagctgatc
     5281 gacgccgtcg cccggcgcgc cgtggcgaag ccggagacgg cagcagaatg gaagcggctg
     5341 cacgccgcgc tccagcaggt gcgggtcacc gcgaccgtcg agcgatgcgc gcgcgagccg
     5401 gcggagatcg cgcggtactc gctggtcacc gggcacaact ggcacacctg gaccgagcgg
     5461 cccgcggcgc tctcccccgg gccggcgcgc cggc
//
LOCUS       DASVOR010002022         2548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_226112,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002022 DASVOR010000000
VERSION     DASVOR010002022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226112"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VF516_37560"
     CDS             <1..444
                     /locus_tag="VF516_37560"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8113517.1"
                     /translation="VEDTSRGSVHELRGQPVIRYWLSERDVAHIKRGLDVLAQIFFAA
                     GARLLHMPVAGFDVLRPDDLPALRRARLRPWDLDISAYHPLGTARMGRDPASSVVDAN
                     HQVHDVPALYVVDGSAVPTAIGVNPQVTIMAMATRAAEKIAHALA"
     gene            complement(471..1529)
                     /locus_tag="VF516_37565"
     CDS             complement(471..1529)
                     /locus_tag="VF516_37565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEX8113518.1"
                     /translation="MAEIAFPETGLEFGVFDWIESSAAAPAEIFEHKLQIAEVADAAG
                     FRGWHLAEHQGTPLSIDGAPSLMIAAAAQRTRRLRLGALTWCLPWYNPYRFYSEICML
                     DHMSGGRLDLGVGRGVSLIESEFMGIRGVEESRARYRETLDIFFAACGAAVLEFEGRY
                     HSYKGLELQLKPLQQPYPPLWFPSSDPSSIEFTARHGYHTVVIGPPRAAKGLFERYRE
                     AWVRHRGEPGRHNAHVAAPKLGKLQHVLVAETDAEAMTAGLPAFRAWADHIHYLTRQR
                     GLPDLMRNMDPASPDAVQRLVAGAPNTVAEQLADIVRDSGTNYLLLVFSFGDLALERA
                     MHSMQLFVREVMPALRSV"
     gene            1635..2477
                     /locus_tag="VF516_37570"
     CDS             1635..2477
                     /locus_tag="VF516_37570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113519.1"
                     /translation="MRAKLVMLALWLAAAPAAADSKTKELARSYEKELAACEMRLHGI
                     ERVTAGTQALVDDGQRQYEADLAALRTGLTEMQAYDAELTATLQILNADPGAPYRSLE
                     RKLDEQDNKIRKLRQTTKKVLENLAPVTARMIPRINARTGTPAPVVKHVHIQFASGRA
                     IDAPLLTGTYRTLGTDATDAVEYGEGKDSAKVTTKLIANATCEQQRQTIAADATDVPA
                     SGAVQSLGLAWYVGTARSGRRLRAACRATKAGAVLATIDEPAGVSGWPELEPVLAAMV
                     AARP"
BASE COUNT          409 a          891 c          923 g          325 t
ORIGIN      
        1 gtcgaggaca cctcgcgcgg ctcggtccac gagctgcgcg gccagcccgt gatccgctac
       61 tggctgtccg agcgcgacgt cgcgcacatc aagcgcggcc tcgacgtgct cgcccagatc
      121 ttcttcgccg ccggcgcccg cttgctccac atgccggtcg ccgggttcga cgtgctccgc
      181 cccgacgacc tgcccgcgct gcgccgcgcc cggctccggc cgtgggacct cgacatctcg
      241 gcctatcacc cgctcggcac cgcgcgcatg gggcgcgatc cggcgagctc ggtggtcgac
      301 gccaaccacc aggtccacga cgtccccgcg ctctacgtcg tcgacggctc cgcggtgccg
      361 accgcgatcg gcgtcaaccc gcaggtcacc atcatggcga tggcgacccg cgccgccgag
      421 aagatcgccc acgccctggc atagcgctgc ggctcgctcc catggccgag ctagaccgag
      481 cgcagcgccg gcatcacctc gcgcacgaac agctgcatcg aatgcatcgc ccgctcgagc
      541 gcaaggtcgc cgaacgagaa caccagcagc aggtaattgg taccggagtc gcgcacgatg
      601 tccgcgagct gctcggcgac cgtgttcggc gcccccgcga ccaggcgctg caccgcatcg
      661 ggcgaagcgg ggtccatgtt ccgcatgaga tcgggcaggc cgcgctgacg ggtgaggtag
      721 tggatgtggt cggcccaggc gcggaacgca ggcaggcccg cagtcatcgc ctcggcgtcg
      781 gtctccgcga ccagcacgtg ctgcagcttt cctagcttgg gcgccgcgac gtgcgcgttg
      841 tgacgcccgg gctcgccgcg gtggcgcacc caggcctcgc ggtagcgctc gaacagaccc
      901 ttcgccgccc tgggcgggcc gatgacgacg gtgtggtagc cgtggcgcgc ggtgaactcg
      961 atcgagctgg ggtcgctgct cggaaaccac agcggcggat agggctgctg cagcggcttg
     1021 agctgcagct ccagtccctt gtaggaatgg taacggccct cgaattcgag cacggcggcg
     1081 ccgcaggcgg cgaagaagat gtcgagtgtc tcgcggtagc gtgcgcgcga ctcctcgacg
     1141 ccgcggatcc ccatgaactc ggactcgatc agggacaccc cccgccccac gcccagatcg
     1201 agccggccgc cgctcatgtg gtcgagcatg cagatctcgc tgtagaagcg gtaggggttg
     1261 taccagggca ggcaccaggt gagcgcgccc aggcgcaggc gccgcgtgcg ctgcgccgcg
     1321 gccgcgatca tgagcgaagg cgcgccgtcg atcgacagcg gcgtgccctg gtgctcggcg
     1381 aggtgccagc ctcggaaccc cgccgcgtcc gctacttcgg cgatctgcag cttgtgctcg
     1441 aagatctcgg cgggtgcggc ggcggaagac tcgatccagt cgaagacacc gaattcgagg
     1501 ccggtctcgg ggaaggcaat ctcggccatg gtgccggggc gatggtacgg tgcccgcggc
     1561 gagcgaggaa gacccgaggt gagcgcgctg gcgatgccgg cgtccaccgc agcgcgccgc
     1621 tgatacaatg gggcatgcgc gcaaagctcg tgatgctggc gctgtggctc gccgcggcac
     1681 cggcggccgc ggactcgaag accaaggagc tcgccaggag ctacgagaag gagctcgcgg
     1741 cgtgcgagat gcgcctgcac ggcatcgagc gggtcaccgc cggcacccag gccctggtcg
     1801 acgacggcca gcgccagtac gaggccgacc tcgccgcgct gcgcaccggg ctgaccgaga
     1861 tgcaggcgta cgacgccgag ctcacggcga cgctccagat cctgaacgcc gatcccggcg
     1921 caccctaccg atcgctggag cgcaagctcg acgagcagga caacaagatc cgcaagctgc
     1981 gccagaccac gaagaaggtg ctggagaatc tcgcgccggt gacggcacgg atgatcccgc
     2041 ggatcaacgc gcggaccggc acgccggcgc cggtggtgaa gcacgtgcac atccagttcg
     2101 cgagcgggcg agcgatcgac gcaccgctgc tcaccgggac ctaccggacg ctgggcacgg
     2161 acgcgaccga tgccgtcgag tacggggagg gcaaggacag cgcgaaggtc accacgaagc
     2221 tgatcgcgaa tgcgacgtgc gagcagcagc gccagacgat cgcggcggac gcgaccgacg
     2281 tcccggcgtc gggcgcggtc cagtcgctcg ggctggcgtg gtacgtcgga accgcgagga
     2341 gcggccggcg gctgcgcgcg gcgtgccgcg cgaccaaggc gggtgcggtg ctcgccacga
     2401 tcgacgagcc ggccggtgtc agcgggtggc ccgagctcga gccggtgctg gcggcgatgg
     2461 tcgcggcgcg gccgtgaccg agcgatccgc tcagaggtcg atccgctcgc gccaggtggc
     2521 ggcggcgccg ggggagaacc ggacacca
//
LOCUS       DASVOR010002023         2147 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_138331,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002023 DASVOR010000000
VERSION     DASVOR010002023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138331"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1421)
                     /locus_tag="VF516_37575"
     CDS             complement(261..1421)
                     /locus_tag="VF516_37575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MYXO-CTERM sorting domain-containing protein"
                     /protein_id="HEX8113520.1"
                     /translation="MSVPVMTIEGDVVAVHSYWTDDGSRIETDATIETIDGQQVVVNQ
                     FGGTVDGIGMIQMPGERILELGMHVAVAAHTGLDQLQQEHVVLDSAKIITDVPGFVRT
                     GPTNSGHYLYWESGCAFVTIDAAGTTAQPAAQTAKTIQDSIDEWNTKTASCSYFQLMN
                     AGTKAREVGNDMVNLIKFHDTPCTCDGGGTSWGCRPATKTSAAKCYSPSAAAITTATY
                     VNDPNSPRDGAIVDADIELNGVDFDITVNGTTHTPTGHCSADLQNTLTHELGHLHGLE
                     HTCRLNASDPQWIDDQGNAVPACSPTLPPKILDATMYPQQDCGETKKSSLSDDDIQAI
                     CTIYPTAKNPGTCEPVGGTNGGCCSASRGADRPAGALLLAGVVALWMRRRRK"
     gene            1462..>2147
                     /locus_tag="VF516_37580"
     CDS             1462..>2147
                     /locus_tag="VF516_37580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX8113521.1"
                     /translation="MTASGKPVRVRIAPSPTGDPHVGTAYIALFNYVFARQQGGKFVL
                     RIEDTDQSRARTDSEQLIFDALHWVGLTWDEGPDVGGPHAPYRQSERGEIYRTHAGIL
                     LERGEAYRCFCTEDRLAKVRIQQQAEKRTLGYDRHCRDLDPGDGARRAAAGEPHVIRL
                     KVPVSGPITFRDELRGEVTRDAAEIDDQVLLKSDGLPTYHLANVVDDHLMEISHVIRA
                     EEWISSTQKHV"
BASE COUNT          325 a          743 c          727 g          352 t
ORIGIN      
        1 gaccccgacc gcgaccacca ccgccgagat cggaaacccg cgctgcacga cggccgtgcc
       61 gcccggatcc gcggtggtgc gctgggcgcg cgcgaccggc agccccgcgc gacacctcgg
      121 acacctcacg taacctggca ccacggcgac gccgcaggcc gggcaaaccg agctcatact
      181 cccgaggata acgcgaggcg agtcgccccg cgaattcttc gctcggcggg tctcgacctg
      241 ctgcgcagga gccggatccg tcacttcctc cggcgccgca tccagagcgc gaccaccccc
      301 gccagcagca gggcgccggc cgggcggtcg gcgccgcgcg acgcgctgca acatccgccg
      361 ttggtccctc cgaccggctc gcaggtcccg gggttcttgg cggtgggata gatcgtgcag
      421 atcgcctgga tgtcgtcatc ggacagcgac gacttcttgg tctcgccgca gtcctgctgg
      481 ggatacatcg tggcgtcgag gatcttcggg ggcagcgtcg gcgagcacgc cggcaccgcg
      541 ttgccctggt cgtcgatcca ctgcgggtcc gacgcgttca ggcggcaggt gtgctccagc
      601 ccgtgcaggt gaccgagctc gtgggtcagc gtgttctgca gatcggcgga gcaatgcccc
      661 gtcggggtgt gcgtcgtacc gttgaccgtg atgtcgaagt cgacgccgtt gagctcgata
      721 tcggcgtcga cgatcgcgcc gtcgcgcggg ctgttcgggt cgttgacata ggtcgccgtg
      781 gtgatcgccg ccgccgacgg cgagtagcac ttcgccgccg aggtcttggt cgccggccga
      841 cagccccacg acgtgccgcc gccgtcgcag gtgcacggcg tgtcgtgaaa cttgatcagg
      901 ttgaccatgt cattgccgac ctcgcgcgcc ttcgtgcccg cgttcatgag ctggaagtac
      961 gagcacgatg cggtcttggt gttccactcg tcgatcgagt cctggatggt cttggccgtc
     1021 tgggctgcag gctgtgccgt cgtcccggcg gcgtcgatgg tgacgaacgc gcagcccgac
     1081 tcccagtaca ggtagtgccc gctgttggtc ggtccggtgc ggacgaagcc gggcacgtcg
     1141 gtgatgatct tcgcgctgtc gagcacgacg tgctcctgct gcaactgatc gaggccggtg
     1201 tgggcggcga ccgcgacgtg catcccgagc tcgaggatcc gctcgccggg catctggatc
     1261 atcccgatgc cgtcgacggt gccgccgaac tggttgacga cgacctgctg gccgtcgatg
     1321 gtctcgatgg tcgcgtcggt ctcgatccga gagccgtcgt cggtccagta gctgtgtacg
     1381 gcgaccacgt cgccttcgat cgtcatcacg ggcacggaca tggcctgacg acgctagcat
     1441 gccgtgttac aagggcgcac catgacggcc tccggcaaac cggtgcgtgt gcggatcgca
     1501 ccatcgccga ccggcgaccc ccacgtcggc accgcgtaca tcgcactgtt caactacgta
     1561 ttcgctcgcc agcagggtgg caaattcgtg ctgcgcatcg aggacaccga tcagtcccgg
     1621 gctcgcaccg actccgagca gctgatcttc gacgcgctcc actgggtcgg cttgacgtgg
     1681 gatgaaggtc ctgatgtagg cgggcctcac gccccgtatc gccagagcga gcggggcgag
     1741 atctaccgca cgcacgcggg catcctgctc gagcgcggcg aggcatatcg ctgcttctgc
     1801 accgaggacc ggctcgccaa ggtccggatc cagcagcagg ccgagaagcg cacgctgggc
     1861 tacgaccggc actgccgcga tctcgacccg ggcgacggcg cccgccgcgc cgcggccggc
     1921 gagccccacg tgatccgcct caaggtcccg gtgtcgggcc cgatcacgtt ccgcgacgag
     1981 ctgcgcggcg aggtcacgcg cgacgccgcc gagatcgacg accaggtgct gctcaagtcc
     2041 gacggcctgc cgacctacca cctcgccaac gtggtcgacg atcacctgat ggagatcagc
     2101 cacgtgatcc gcgccgagga gtggatctcg tcgacccaga agcacgt
//
LOCUS       DASVOR010002024         2959 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_342429,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002024 DASVOR010000000
VERSION     DASVOR010002024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342429"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2015)
                     /locus_tag="VF516_37585"
     CDS             complement(<1..2015)
                     /locus_tag="VF516_37585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8113522.1"
                     /translation="MKLDKLTVKAQEAIAASRDVAMSLHHAEVRPEHLLVALLEQEGG
                     VVPRILAKIGADPRVVRADLDRALEKLPRARGAALDVEAGRAFKDVWEQAGREAEAMK
                     DEYISTEHFLIALAASKTPAGEALTRAGATKEAILEALIAVRGNQRVQDQDPEAKYEA
                     LDRYTRDLTALARADKLDPVIGRDEEIRRTIQILSRRTKNNPVLVGEAGVGKTAVVEG
                     IAQRIAKGDVPESLRDKRIVSLDLGALIAGAKYRGEFEERLKAVLKEVAAAAGQVIMF
                     IDELHTLVGAGAAEGATDAANLLKPALARGDLRCIGATTLDEYRKHIEKDKALERRFQ
                     PVMVEEPSVTDTIAILRGLKDKYEVHHGIRIRDGALVAAARLSHRYIPQRQLPDKAID
                     LVDEAASRLKMEIESVPTPIDMLERRVMTLEVERAALERENDRTAKQRLPEVRREIAE
                     LKEQASAMKAQWQRERDLIRDLRTKKEKLDQLKAEAERLQRQGDYARASELRFGSIPE
                     LEKDIARDTAQADELRAKGSFLREEVTEDDIATVVARWTGIPVEKMLEGEVERLNKME
                     DRLRQRVVGQDAAVAAVAAAVRRARAGLKDPDRPIGSFLFLGPTGVGKTELARALAEF
                     LFDDEHAMIRIDMSEYQEKHTVSRLVGAPPGYIGYDQGGQLTEAVRR"
     gene            complement(2085..>2959)
                     /locus_tag="VF516_37590"
     CDS             complement(2085..>2959)
                     /locus_tag="VF516_37590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113523.1"
                     /translation="LGRDSEALQLAQLAGSLAPGRRDAMTRVQELATRVGELHLALAA
                     ARQVLELIPLDDDEAQLAVRFALVELLREAGSLDDAVGELDKVLRDHPVHAGAIETLA
                     HVHMARGDWMAATRYLYQLVPLAPSPTHRAERLYQLGEAVLVHVNDLDRADDVFLRAS
                     DLDPGHVPTLRRLLDVYWRADDPGGIVEVATELADKGALINGPTSEIALARALVAAAL
                     GGDTEHAQQLQTALGDETARSIAEALAELVGRTGRLQLGTASTAVAELARRGYLDLAK
                     LRAAMAGTAAANTL"
BASE COUNT          352 a         1064 c         1075 g          468 t
ORIGIN      
        1 tggcggcgga ccgcctcggt gagctggccg ccctggtcgt agccgatgta gccgggcggc
       61 gcgccgacca gccgcgacac ggtgtgcttc tcctggtact cggacatgtc gatccggatc
      121 atcgcgtgct cgtcgtcgaa caggaactcg gcgagcgcgc gcgccagctc ggtcttgccg
      181 acgccggtcg gccccaggaa caggaagctg ccgatcggcc ggtcggggtc cttgagcccg
      241 gcgcgcgcgc ggcggaccgc ggcggcgacc gcggcgaccg cggcgtcctg accgaccacg
      301 cgctggcgca gccggtcctc catcttgttg agccgctcga cctcgccctc gagcatcttc
      361 tcgaccggga tcccggtcca ccgcgcgacc accgtggcga tgtcgtcctc ggtgacctcc
      421 tcgcgcagga agctgccctt ggcgcgcagc tcgtcggcct gggcggtgtc gcgcgcgatg
      481 tccttctcga gctcggggat cgagccgaac cggagctcgg acgcccgcgc gtagtcgccc
      541 tggcgctgca gccgctcggc ctcggccttg agctgatcga gcttctcctt cttggtgcgc
      601 aggtccctga tcaggtcgcg ctcgcgctgc cactgcgcct tcatcgccga cgcctgctcc
      661 ttgagctcgg cgatctcgcg ccggacctcg ggcaggcgct gcttggccgt gcggtcgttc
      721 tcgcgctcga gcgcggcgcg ctcgacctcc agcgtcatga cccggcgctc cagcatgtcg
      781 atcggggtcg gcacgctctc gatctccatc ttgagccggg acgcggcctc gtcgaccagg
      841 tcgatcgcct tgtcgggcag ctggcgctgc gggatgtagc ggtgcgacag ccgggcggcg
      901 gcgaccagcg cgccgtcgcg gatccggatg ccgtggtgga cctcgtactt gtccttgagg
      961 ccgcgcagga tcgcgatcgt gtcggtgacc gacggctcct cgaccatcac cggctggaac
     1021 cggcgctcga gcgccttgtc cttctcgatg tgcttgcggt actcgtcgag cgtggtcgcg
     1081 ccgatgcagc gcagatcgcc gcgcgcgagc gcgggcttga gcagattcgc cgcgtcggtc
     1141 gcgccctcgg cggcgccggc gccgaccagg gtgtgaagct cgtcgatgaa catgatgacc
     1201 tggccggcgg cggcggcgac ctccttgagc acggccttga gccgctcctc gaactcgccg
     1261 cggtacttgg cgcccgcgat cagcgcgccg aggtcgagcg agacgatccg cttgtcgcgc
     1321 agcgactcgg ggacgtcgcc cttggcgatc cgctgggcga tgccctcgac caccgcggtc
     1381 ttgccgacgc cggcctcgcc gaccagcacc gggttgttct tggtccgccg cgacaggatc
     1441 tggatcgtcc ggcggatctc ctcgtcgcgc ccgatcaccg ggtcgagctt gtcggcccgg
     1501 gcgagcgcgg tcagatcgcg ggtgtagcga tcgagcgcct cgtacttggc ctccgggtcc
     1561 tggtcctgga cccgctggtt gccgcggacc gcgatcagcg cctcgaggat cgcctccttg
     1621 gtggcgccgg cccgggtcag ggcctcgccg gcgggcgtct tgctcgccgc cagggcgatc
     1681 aggaagtgct cggtcgagat gtactcgtcc ttcatcgcct cggcctcgcg gcccgcctgc
     1741 tcccagacgt ccttgaacgc gcggccggcc tcgacgtcga gcgccgcgcc gcgggcgcgc
     1801 gggagcttct ccaacgcgcg atcgaggtcg gcgcggacca cgcgcgggtc ggcgccgatc
     1861 ttggccagga tccgcggcac cacgccgcct tcctgttcga gcaaggcgac caggaggtgc
     1921 tcggggcgga cctccgcgtg gtgcagcgac atcgcgacat cgcgcgacgc ggcgatcgcc
     1981 tcttgggcct tgacggtgag cttgtcgagc ttcatgacgg ctccaacgta accacgctta
     2041 catagccgtc aatactccag gtgctcgaag gggttagcgc gcaattatag cgtgttcgcc
     2101 gccgccgtcc ccgccatcgc cgcccgtaac ttggcgagat ccaggtagcc tcgccgggcg
     2161 agctcggcga ccgcggtcga ggccgtcccg agctggagcc ggccggtgcg cccgaccagc
     2221 tcggcgaggg cctcggcgat gctccgagcc gtctcgtcgc cgagcgcggt ctgcagctgc
     2281 tgggcgtgct cggtgtcgcc gcccagcgcc gccgcgacca gggcgcgcgc cagggcgatc
     2341 tcggaggtcg ggccgttgat cagcgcgccc ttgtcggcga gctcggtcgc gacctcgacg
     2401 atcccgccgg gatcgtcggc gcgccagtag acgtcgagca gccggcgcag cgtcgggacg
     2461 tggccgggat cgagatcgga ggcgcgcagg aacacgtcgt cggcgcggtc gaggtcgttg
     2521 acgtggacca gcaccgcctc gccgagctgg tacagccgct cggcgcgatg ggtcggcgac
     2581 ggcgcgagcg ggaccagctg atagaggtag cgggtcgcgg ccatccagtc gccgcgcgcc
     2641 atgtggacgt gggccagggt ctcgatcgcg ccggcgtgca ccgggtgatc gcgcagcacc
     2701 ttgtcgagct cgccgaccgc gtcgtcgagg gagccggcct cgcgcagcag ctcgaccagc
     2761 gcgaaccgga ccgcgagctg ggcctcgtcg tcgtcgagcg ggatcagctc caggacctgg
     2821 cgcgccgccg cgagcgccag gtgcagctcg ccgacccggg tcgcgagctc ctggacccgg
     2881 gtcatcgcgt cgcgccggcc cggggcgagg ctgccggcga gctgggcgag ctggagcgcc
     2941 tcgctgtcgc ggccgaggt
//
LOCUS       DASVOR010002025         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_196962,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002025 DASVOR010000000
VERSION     DASVOR010002025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196962"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..781)
                     /locus_tag="VF516_37595"
     CDS             complement(<1..781)
                     /locus_tag="VF516_37595"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEX8113524.1"
                     /translation="MAKNRGTDAQLLTMHELAAYLHLDEATVTKLVMQGKIPSLQVDR
                     QWRFKRTAIDEWIEQQLVGEDENFADVPDGMKLPLDDLLPDQAIITNLRARTPVGVIE
                     ELAARAYTNGWLSDKPWFVGAVVERESLASTAMEGGVAFLHTRAKDKGKIGRPFIVVG
                     RSWEGIMFGAPDNAPTYLFFLLGLKYDRLHLPILGRLARALRNPATIAKLRALSSPDQ
                     VRALLLKEDAAVRMGHTPEIQAPAAFKPKLDRTLRLRAIRRL"
     gene            862..1746
                     /locus_tag="VF516_37600"
     CDS             862..1746
                     /locus_tag="VF516_37600"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEX8113525.1"
                     /translation="MQRPRWSLRRARTLVGTKFMLIAGLLGFVVFAWLVELVAGLRDP
                     VNLGPLLAAIMAGIPAVFWLGFFYLMDRHEPEPKQLVAGVCVLGALIAAPLADFVHSE
                     FAPPAALGVAGLSPFSLDRVLDAVLVVGLSQEMSKYAVVRYTIYMSREFDEPMDGIVY
                     MMACGTGFAVWVNFHRLSGQNHEVYLSIGAAQAVVTTLAHASFAGVLGYVMGRAKFSR
                     RRAPARGILVMLGLLTAAAFNGQFTLVTGWVVQRGLAVHPWYSVGYAALSAAAIFGII
                     WLVSQRLLALSPFRDTAA"
BASE COUNT          254 a          630 c          685 g          318 t
ORIGIN      
        1 gcagccggcg gatcgcgcgc agccggagcg tgcggtcgag cttgggcttg aacgccgccg
       61 gggcctggat ctcgggagtg tgtcccatgc gcacggcggc gtcctccttg agcaggagcg
      121 cacgcacctg gtccggcgag ctcagcgcgc gcagcttggc gatcgtcgcc gggttgcgca
      181 gcgcgcgtgc cagccgcccg aggatcggga ggtggaggcg gtcgtacttg aggccgagca
      241 ggaagaacag gtacgtcggg gcgttgtccg gcgcaccgaa catgatgccc tcccacgagc
      301 ggccgacgac gatgaacggc cggccgatct tgcccttgtc cttggcgcgg gtgtgcagga
      361 aggcgacgcc gccctccatc gcggtcgacg ccagtgactc gcgctcgacc accgcgccca
      421 cgaaccacgg cttgtcagac agccagccgt tggtgtacgc gcgtgcagcg agctcctcga
      481 tcacaccgac cggggtgcgc gcccggaggt tggtgatgat cgcctgatcg gggagcaggt
      541 cgtcgagcgg gagcttcatg ccgtccggca catccgcgaa gttctcgtcc tcgccgacca
      601 gctgctgttc gatccactcg tcgatcgcgg tgcgcttgaa ccgccactgg cgatcgacct
      661 gcagcgaggg aatcttgccc tgcatcacga gcttggtgac ggtcgcctca tcgaggtgaa
      721 ggtaggccgc cagctcgtgc atggtcagca actgcgcgtc ggtgccgcga tttttagcca
      781 tagaacccgt cgaaaaaaca gcagcccgat tctagggggg gccgtggtcc gcgcaaagct
      841 cgggcagcgt agaatttcgc catgcagagg ccgcgctggt cgctccgccg agcccggacg
      901 ctggtcggca ccaagttcat gctgatcgcc gggctgctgg ggttcgtcgt gttcgcctgg
      961 ctcgtcgagc tcgtcgcggg cctgcgcgat ccggtgaacc tggggccgct gctcgctgcg
     1021 atcatggccg ggatcccggc ggtgttctgg ctcgggttct tctacctgat ggaccgccac
     1081 gagcccgagc ccaagcagct ggtggccggg gtgtgcgtgc tcggcgcgct gatcgccgcg
     1141 ccgctcgccg acttcgtgca ctcggagttc gcgccgccgg cggcgctcgg ggtcgccggg
     1201 ctgtcgccgt tctcgctcga ccgcgtgctc gacgcggtgc tggtcgtcgg gctgtcgcag
     1261 gagatgtcca agtacgccgt ggttcgctac accatctaca tgtcgcggga gttcgacgag
     1321 ccgatggacg gcatcgtcta catgatggcc tgcggcaccg gcttcgcggt gtgggtcaac
     1381 ttccaccggc tgtcgggtca gaaccacgag gtgtacctgt cgatcggcgc ggcgcaggcc
     1441 gtggtgacca cgctggccca cgcctcgttt gccggcgtgc tgggctacgt gatggggcgc
     1501 gccaagttct cgcgccggcg ggcaccggcg cgcggcatcc tggtgatgct gggcctcttg
     1561 accgcggccg cgttcaacgg gcagttcacg ctcgtcaccg gctgggtcgt gcagcgcggg
     1621 ctcgcggttc acccgtggta cagcgtgggc tacgccgcgc tgagcgccgc ggcgatcttc
     1681 gggatcatct ggctggtgtc gcagcgcctg ctcgcgctgt cgccgttccg ggatacggcc
     1741 gcatgaccga cgagaagtcc aagagcggcc ggcgatcgcg tgagacgccg gtcgccggag
     1801 cctcgcaggt cgcgccgccg gacgccgccg agggggccga ggccgccacc gagacccggg
     1861 cggccgtcga ggcacaagcc gcgctcc
//
LOCUS       DASVOR010002026         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_429997,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002026 DASVOR010000000
VERSION     DASVOR010002026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429997"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VF516_37605"
     CDS             complement(<1..873)
                     /locus_tag="VF516_37605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113526.1"
                     /translation="MRLPTALRGGKRSFALFAIVAIPTVAHVWLSRAGYSPTDDGLIL
                     ALARRLVSGEVPHRDFISVRPVGSGLVHAPFLLAGELGYWTSRWFVWCQFGAIAWACV
                     RILESYTQPLGGRRFALAVLVFIATAHYFPPMAWHTIDGVCATCVGVALVRTRGGWAR
                     MLGYVLIGFSCICKQGFVVSATVTFVALGGHRSLRHVVAVLAPGALYVIVLGLAGGLA
                     DAVQQLTMTSGPLLDGMYELYTGLNSLVGIVLGILVWLVDRWAGSRAARWARALVVAP
                     LFSGLVVRVLQWPRP"
     gene            1090..1329
                     /locus_tag="VF516_37610"
     CDS             1090..1329
                     /locus_tag="VF516_37610"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8113527.1"
                     /translation="MSAAIVKQLLASALGVSVERIGDDAARGKLRNWDSVGHVDVITA
                     IEDHFAVQLDDAELLRMTSLPEIVAVLRAKGVSLD"
     gene            complement(1321..>2042)
                     /locus_tag="VF516_37615"
     CDS             complement(1321..>2042)
                     /locus_tag="VF516_37615"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8113528.1"
                     /translation="LLGGSRFSYGVYEAVQRNLPNARCYNLYGPTECTVYCHAKTLSG
                     DPAHDMLDFNVSVGQPIRGTTCLVMNPAGGVITTPREPGELWIGGVQVLDEYRGSPEV
                     TANAVVTYQGERYYRTGDVGFFDDRGDYYITGRLDETLKRRGYRVNLLNIDSYIQRLA
                     PVQECMTIAVPHPVYEHELVTYVVARAAVTEDELLQLMRQVLVDYEIPDEIRFAQELP
                     RGATGKVDRKQLELRYATKSV"
BASE COUNT          365 a          674 c          717 g          286 t
ORIGIN      
        1 ggggcgcggc cactgcagaa cgcgaacgac gaggccggag aacagcggtg caaccacgag
       61 cgcacgcgcc cagcgcgcgg ctcgggagcc ggcccagcgg tcgacgagcc acaccagaat
      121 gccgaggacg ataccgacca gcgagttgag gcccgtgtac agctcgtaca tcccatcgag
      181 gagcgggccg ctggtcatcg tcagttgctg cacggcgtcg gcaaggccac cggcgagacc
      241 gagcacgatg acgtagagtg cgccgggcgc gagcacggcg acgacgtggc gcaacgagcg
      301 atgccccccg agcgcgacga acgtgacggt ggcggagacc acgaagccct gcttgcagat
      361 gcagctgaag ccgatcagga cgtagccgag catgcgtgcc cagcccccgc gcgtgcgcac
      421 cagcgcgaca cccacgcagg tggcgcacac gccgtcgatc gtatgccatg ccatcggcgg
      481 aaagtagtgg gcggtcgcga tgaacaccag gacggcgagc gcgaagcgcc ggccgccgag
      541 cggctgcgtg tagctctcga ggatccgcac gcacgcccag gcaatcgcac cgaactggca
      601 ccacacgaac cagcgcgacg tccagtagcc tagctcgccg gcgagcagaa acggcgcgtg
      661 caccagtccc gagccgaccg ggcgtaccga gatgaagtcg cgatgcggga cctcgccgga
      721 gaccagccgg cgcgcgagcg cgaggatcag cccgtcgtcg gtcggcgagt accccgcacg
      781 cgatagccag acatgggcga cggtcggtat ggcgacgatc gcgaacaacg cgaaggagcg
      841 cttgccgccg cgcaacgcag tggggagccg caccgcgcgc gaggttagca tggccggtgc
      901 acgacccgtt tgccgggtgc ccggcggtcg tctcgagctc gttgacgagc gcggagccgc
      961 gcacgttcct gacgcgcggc gtcgcgacgc cgatcggccg cgagctcggc tcacacggag
     1021 tggccgccgg gatgacgatg cgcgcaccgt tgctgttccc agccgcacac ttcgcgtagc
     1081 attgacggga tgagcgccgc gatcgtgaag cagctgctgg cgagcgcgct cggggtctcg
     1141 gtcgagcgca tcggcgatga tgcggcgcgg ggcaagctgc ggaactggga cagcgtcggt
     1201 cacgtggacg tcatcaccgc gatcgaggac cacttcgccg tgcagctcga cgatgccgag
     1261 ctcttgcgga tgacgtcgct gcccgagatc gtcgccgtgc tacgcgccaa gggcgtgtcg
     1321 ctagactgac ttcgtcgcgt accgaagctc tagctgcttg cgatcgacct tccccgtcgc
     1381 gccgcggggc agctcctgtg caaatcggat ctcatcggga atctcgtagt caacgagcac
     1441 ctggcgcatc aactgcaaca gctcgtcctc ggtgacggcg gcgcgcgcca cgacgtaggt
     1501 cacgagctcg tgctcgtaca ccggatgcgg cacggcgatc gtcatgcact cctgcaccgg
     1561 cgcgaggcgc tggatatacg agtcgatgtt caagaggttg acgcggtagc cgcgtcgttt
     1621 gagggtctcg tcgagccggc cggtgatgta gtagtcgccg cggtcatcga agaagccaac
     1681 gtctcccgtt cgataatagc gctcgccctg gtacgtgacg acggcgttcg cggtcacctc
     1741 ggggctgcca cggtactcgt cgagcacctg gacgccgccg atccacaact cgcccggctc
     1801 gcgtggtgtc gtgatgacgc cgccggctgg attcatcacc aggcacgtcg tgccgcggat
     1861 cggctggccg acggagacgt tgaagtcgag catgtcgtgc gcggggtcgc cggacagcgt
     1921 cttggcgtgg cagtacacgg tgcactcggt cggcccatac aggttgtaac agcgcgcgtt
     1981 cggcaggttg cgctggacgg cctcgtagac accatacgag aaccggctgc ccccgaggag
     2041 ca
//
LOCUS       DASVOR010002027         2587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_226207,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002027 DASVOR010000000
VERSION     DASVOR010002027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226207"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..183)
                     /locus_tag="VF516_37620"
     CDS             complement(<1..183)
                     /locus_tag="VF516_37620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04563 family protein"
                     /protein_id="HEX8113529.1"
                     /translation="MAGSDKRKQSLYFPEDMLKEIQDEATRQDRSLSWIVQKAWKIAR
                     KEIMKYPSVNEFPGEED"
     gene            433..837
                     /locus_tag="VF516_37625"
     CDS             433..837
                     /locus_tag="VF516_37625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HEX8113530.1"
                     /translation="MSTTTATPPSSKQLTKKELKKFQELLEEKRKAVLDRAREMLSVE
                     NMALDTNDLPDEMDLASSEYLQSFEFRLRGREKSLLSKLDLALKKITDGTFGICEICE
                     EPIGKKRLEARPETTLCIKCKEDQEREERTMG"
     gene            1095..1619
                     /locus_tag="VF516_37630"
     CDS             1095..1619
                     /locus_tag="VF516_37630"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8113531.1"
                     /translation="MPDEDRRKFLKVATCAIGGGVGLAVAGPALTLLASPAGKITVTT
                     PTAPLDLGPAEQFRVGAEPRKVDIVAPVIKDAWTAARNVVLGAAWVRRNAQDKLVALS
                     AICPHLGCGIDWEPITGNFLCPCHDSRFSPDGAKLTGPSERGMDPLPLEIKDGRVQLT
                     WIRYKLGSTTPEPA"
     gene            1616..>2587
                     /locus_tag="VF516_37635"
     CDS             1616..>2587
                     /locus_tag="VF516_37635"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HEX8113532.1"
                     /translation="MTDKIRTYLRERGLSGAGAPATIGEASFAYVFGAVLMFLLALQA
                     VTGVAIAAFYAPASTDAWASVAYVQDQVTLGWFLRGVHYHGASALVIVAGLHLVQTAV
                     IGAYKKPRELVWWLGLLLMVLLLAWAVTGYVLRWDQAGFYANKVEMEITAATPLVGGL
                     VKKLALGGNDYGNLTLTRFYALHVLFLPAIVGAVTWGHVAVARKLGATPLHSRERPGV
                     PRWPEQTLRNTVAIAMVLTVLVVYVVAVHGVELAAPADPSQAYDARPLWYFRWLFELR
                     ELSGRAEKVVALVVPAIVAGALVALPLVDREPTRALRSRLPYLGGLAG"
BASE COUNT          397 a          882 c          879 g          429 t
ORIGIN      
        1 gtcttcctcg cccgggaact cgttgaccga cgggtatttc atgatctcct tccgcgcgat
       61 cttccaagcc ttctggacga tccacgacag ggaccgatcc tgccgggtcg cttcgtcttg
      121 gatttccttg agcatgtctt caggaaagta cagggactgc ttgcgtttat ccgagccggc
      181 catacgcgag ctagtttcga ggaccgccta cacggtgtca agacatccgc ctgtgtatgg
      241 gagaccgatc tgccaaattc tctagccagc tccaccgcct agacgcccag atccccgcat
      301 gccaaccccg cccggcatcc gttttctgag atgcttcgtt cgtggtctgg ggggttgtca
      361 tactcctcgc tcatcactat cctatcgcgc ttttcaaccc ttcggcatct ggccgcagag
      421 ggggcaaaga ccatgagcac cacgacagcc accccaccgt cgtccaagca gctcaccaag
      481 aaggagctga agaagtttca ggagctcctc gaggaaaagc ggaaggcggt cctcgaccgc
      541 gctcgcgaga tgctgtcggt ggagaacatg gcgctcgaca ccaacgacct gccggacgag
      601 atggacctgg cgtcgagcga gtacctccag tcgttcgagt tccggctgcg cggccgggag
      661 aagagcctgc tgtcgaagct ggatctcgcg ctcaagaaga tcacggacgg caccttcggc
      721 atctgcgaga tctgcgagga gccgatcggc aagaagcggc tggaggcgcg gcccgagacc
      781 acgctctgca tcaagtgcaa ggaagaccag gagcgcgaag agcgcacgat ggggtgaggg
      841 ccgagcctcg ttctttccgc gcggcgccga gctcaggacc atgcggctcg tcgcggccgc
      901 catgacgcag ccggcaatcg gcgcatccgg gtgcgctgga tccggagcgg atgtaacgcg
      961 gacacggggg tccgcctcca ttgatccggc acgagtttcg gctacctgca gcccaccgcc
     1021 ggcgcgcgaa ccaacgtgag gaatcgcccg cgcgcggccc acgggccgcc tgtgacctct
     1081 gctaccgtcg cagcatgcct gatgaggatc gcaggaagtt cctgaaggtc gccacgtgcg
     1141 cgatcggagg tggcgtcggc ctggcggtcg ccggtccggc gctgaccttg ctcgccagcc
     1201 cggcgggcaa gatcaccgtg accacgccca ccgcgccgct cgacctcggg ccggccgagc
     1261 aatttcgcgt gggtgccgag cctcgcaagg tcgacatcgt cgcgcccgtc atcaaggatg
     1321 cctggaccgc ggcgcgcaac gtggtcctgg gcgccgcgtg ggttcgccgc aacgcacaag
     1381 acaagctggt cgcgctgtcc gcgatctgtc cgcacctcgg atgcgggatc gactgggagc
     1441 ccatcaccgg caacttcctg tgcccgtgcc acgacagccg gttcagccct gacggggcca
     1501 agctcaccgg cccttcggag cgcgggatgg acccgctgcc cctggagatc aaggatggcc
     1561 gcgtccagct gacgtggatt cggtacaagc tcggctcgac cacgccggag ccggcatgac
     1621 ggacaagatc cggacgtacc ttcgcgagcg cggcctcagc ggcgccggcg ccccggcgac
     1681 catcggggaa gcgtcgttcg cctacgtgtt cggcgcggtg ctgatgttcc tgctcgcgct
     1741 ccaggccgtc accggggtgg cgatcgcggc gttctatgcc ccggcgtcga ccgacgcctg
     1801 ggcctcggtg gcgtacgttc aggaccaggt gacgctgggc tggttcctgc gcggcgtcca
     1861 ctaccacggc gcgtcggcgc tggtgatcgt ggccgggctg cacctggtgc agaccgcggt
     1921 gatcggcgcg tacaagaagc cgcgcgagct ggtgtggtgg ctcggcctcc tgctcatggt
     1981 cctgctcctg gcctgggcgg tgaccgggta cgtgctgcgc tgggatcagg ccgggttcta
     2041 cgccaacaag gtcgagatgg agatcaccgc ggcgaccccg ctggtcggcg gcctggtgaa
     2101 gaagctcgcg ctcggcggca acgactacgg caacctgacg ctgacccggt tctacgccct
     2161 ccacgtcctg ttcctgcccg cgatcgtcgg ggcggtgacc tggggccacg tcgcggtcgc
     2221 ccgcaagctg ggcgcgacgc cgctccacag ccgcgagcga ccgggcgtcc cgcgctggcc
     2281 ggagcagacg ctgcgcaaca cggtcgcgat cgccatggtg ctgaccgtgc tggtggtcta
     2341 cgtggtcgcg gtgcatggcg tcgagctcgc cgcgccggcc gacccgtcgc aggcgtacga
     2401 cgcgcggccg ctgtggtact tccgctggct gttcgagctc cgcgagctgt ccggccgggc
     2461 cgagaaggtc gtcgcgctcg tcgtgccggc gatcgtcgcc ggcgcgctgg tggcgctgcc
     2521 gctggtcgac cgcgagccga cccgcgcgct gcgcagccgg ctgccgtacc tcggcgggct
     2581 cgccggg
//
LOCUS       DASVOR010002028         2368 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_226210,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002028 DASVOR010000000
VERSION     DASVOR010002028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226210"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VF516_37640"
     CDS             complement(<1..499)
                     /locus_tag="VF516_37640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113533.1"
                     /translation="MALGALSIKLRCASWQQLATIYKRDLSHGTMFLRATTAPPVGTV
                     VRIDLTLPSATVIALDGVVAHHVQDPQRGSGIELTLSPIPASSVWLIESALASEARRL
                     ATPAPPALPAIPAIHDHADVAVAEQELVKALVSEAASLKKLNPFLVLGVGYEAGDTEV
                     RAAFGE"
     gene            complement(527..1558)
                     /locus_tag="VF516_37645"
     CDS             complement(527..1558)
                     /locus_tag="VF516_37645"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113534.1"
                     /translation="MEPSAILISPRGVAGPLRDLMDRLGSVGVEVTIVDEVATAVEVS
                     RRHITPPAILLDLRELGAGEIEDQQAAGDAIRRTLERLPHALPIVVTSEADPMLIISC
                     VRAGAGDVIDLQLEGTAAARAVVQRICERQGARAAERARIASQRDMIEELLKDLIRTE
                     RRSIDAEEALAARARTAGEAAVAIESRPPAVLLIEHERAIADELADQLEAAGVATFAF
                     LTGEEALREAERLAHSTGLDLAVVAAQLPGIDGLETVRRLRARIQGLPAFLMTSAHDG
                     ELGERATELGVVGLVRKPLTDLSGVVDRLSRLARDSLHHTREHVYLQRIKERHERVLA
                     RYRSLPREP"
     gene            complement(1558..>2368)
                     /locus_tag="VF516_37650"
     CDS             complement(1558..>2368)
                     /locus_tag="VF516_37650"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8113535.1"
                     /translation="AADRARLATARALLDGWTFATPAATASDARDPDSADSAATAVFH
                     TWMHFFIARTLTDELAAVDFDVWRLDDGQLLRIVDALLDDPKSLVTSPATQQPILCDD
                     YAAPGPDVSCTVQVLAALVDAMTYLESPQGFGTADVRDWRWGALHRLAVAPLLPGAPG
                     LPAPGAPEPGEPAGFPRAGDGFTVSRADPGWSDLDFSELAGPAHRLVAEARPGQPIAV
                     RWALPGGAIYDHRSPHYRDLLDRDYLSDQLFDVPSSIDEIVAAGEVRWVFH"
BASE COUNT          320 a          847 c          903 g          298 t
ORIGIN      
        1 gctcgccgaa cgcggcgcgg acctcggtgt cgccggcctc gtagccgacc ccgagcacga
       61 ggaacgggtt gagcttcttg agcgacgcgg cctccgagac cagcgccttg accagctcct
      121 gctcggcgac cgcgacgtcg gcgtggtcgt ggatggcggg gatcgccggg agcgccgggg
      181 gcgccggcgt cgcgagccgc cgggcctccg aggcgagcgc gctctcgatc aaccacaccg
      241 agctcgccgg gatcggcgac agcgtgagct cgatgccgct gccgcgctgc gggtcctgga
      301 cgtggtgggc gaccacgccg tcgagcgcga tcaccgtggc gctcggcagc gtgagatcga
      361 tgcgcacgac cgtcccgacc ggcggcgcgg tcgtcgcccg caggaacatc gtgccgtggc
      421 tcaggtcgcg cttgtagatc gtggcgagct gctgccacga ggcgcacctg agcttgatcg
      481 agagcgcgcc caaagccatg caaatccact cgagatcata ggccgatcac ggttcgcgtg
      541 gcaacgatcg gtacctcgcc aggacgcgct cgtggcgctc cttgatccgc tgcaggtaga
      601 catgttcgcg cgtgtggtgc agcgagtccc gggcgagccg ggacagccga tcgacgacgc
      661 ccgagaggtc ggtgagcggc ttgcgaacca gcccgacgac gccgagctcg gtggcgcgct
      721 cgccgagctc gccgtcgtgc gccgacgtca tcagaaatgc cgggagaccc tggatccggg
      781 cgcgcagccg gcgcacggtc tccagcccgt cgatcccggg cagctgggcg gcgaccaccg
      841 cgagatcgag cccggtcgag tgcgccagcc gctcggcctc gcgcagcgcc tcctcgccgg
      901 tgaggaaggc gaacgtcgcg acccccgcgg cctcgagctg gtcggcgagc tcgtcggcga
      961 tcgcgcgctc gtgctcgatc agcagcacgg cgggcggccg gctctcgatc gcgaccgcgg
     1021 cctcgccggc ggtgcgcgcc cgggcggcga gcgcctcctc ggcgtcgatc gagcgccgct
     1081 cggtgcggat caggtccttg agcagctcct cgatcatgtc gcgctgcgac gcgatccgcg
     1141 cccgctcggc ggcgcgcgcg ccctggcgct cgcagatccg ctggaccacc gcgcgggcgg
     1201 ccgccgtgcc ctcgagctgg agatcgatca cgtcgccggc cccggcccgc acgcagctga
     1261 tgatcagcat cggatcggcc tcgctggtca cgacgatcgg cagcgcgtgg ggcagccgct
     1321 ccagggtgcg gcggatcgca tcaccggcgg cctgctggtc ctcgatctcg ccggcgccga
     1381 gctcgcggag gtcgagcagg atcgccggcg gcgtgatgtg gcgccggctg acctcgacgg
     1441 cggtcgcgac ctcgtcgacg atcgtgacct cgacgccgac gctgccgagc cggtccatca
     1501 ggtcgcgcag cgggccggcc accccgcggg gcgagatcag gatcgcggac ggctccatca
     1561 gtgaaacacc cagcgcacct cgccggctgc gacgatctcg tcgatcgacg acggcacatc
     1621 gaacagctgg tcggagaggt agtcgcggtc gagcaggtcg cggtagtgcg gcgagcggtg
     1681 gtcgtagatc gcgccgccgg gcagcgccca ccgcaccgcg atcggctgcc ccgggcgggc
     1741 ctcggcgacg aggcggtgcg ccgggccggc gagctcggag aagtcgagat ccgaccaccc
     1801 ggggtcggcg cggctcaccg tgaagccgtc gccggcgcgc ggaaacccgg cgggctcgcc
     1861 gggctcggga gcaccgggcg ccggcaggcc aggggcgccg ggaaggagcg gggcgaccgc
     1921 gagccggtgc aacgcgcccc accgccagtc tcgcacgtcg gcggtgccga agccctgcgg
     1981 cgactccagg taggtcatcg cgtcgaccag cgccgcgagg acctgcacgg tgcagctgac
     2041 gtcggggccg ggcgcggcgt agtcgtcgca caggatcggc tgctgggtcg ccgggctggt
     2101 caccagcgac ttggggtcgt cgagcagcgc gtcgacgatg cggagcagct ggccgtcgtc
     2161 gagccgccac acgtcgaagt cgaccgcggc gagctcgtcg gtgagcgtgc gcgcgatgaa
     2221 gaagtgcatc caggtgtgga acaccgcggt cgccgccgag tctgccgagt cgggatcgcg
     2281 ggcgtcgctc gccgtggccg ccggggtcgc gaacgtccag ccgtcgagca gcgcccgcgc
     2341 ggtggccagg cgcgcccggt cggccgcc
//
LOCUS       DASVOR010002029         2345 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_314153,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002029 DASVOR010000000
VERSION     DASVOR010002029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314153"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="VF516_37655"
     CDS             complement(<1..433)
                     /locus_tag="VF516_37655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113536.1"
                     /translation="MRVWIWLTALVACRSPHVTPAAPPGAVGEAPGPPVASNILRTDY
                     AGSRACADCHGEIYARWEASAMRGMTRDARTAQIRAPFDGATLRVAGDTVTMAMQGGE
                     RIMHVVTARGIDRGNEQTFRVTKVVGGRYREDFVGLDPQGEE"
     gene            complement(475..1119)
                     /gene="gstA"
                     /locus_tag="VF516_37660"
     CDS             complement(475..1119)
                     /gene="gstA"
                     /locus_tag="VF516_37660"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF007831.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEX8113537.1"
                     /translation="MKLYYSPGACSLSPHIVLREAERRFDLERVDLKSHRTASGLDYL
                     TVNPKGYVPALQLDGPGSPILTEGPAIVQYIADLAPDARLVPPSGTFARYHLQEWLNF
                     LSTELHKQFAPLLDPTFPRSAPVAEHLRGKIGRRLLYLQDVLEDRAFLMGETFTVADA
                     YLFTILQWCAPQGIDLGLYPNLDDYEARIADRPTVQAALSAEGLRERHHYYRSA"
     gene            complement(1458..>2345)
                     /locus_tag="VF516_37665"
     CDS             complement(1458..>2345)
                     /locus_tag="VF516_37665"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEX8113538.1"
                     /translation="YGIGGERELPERVLDHLTGFAGTGPVRIGNAAAEQQQNDLNGEL
                     ILCLQTVLSDPRIVIDQPETFFPLIERLVEQAIALAPLADTGIWEFRTLPRHSTFSRA
                     MCWAAIDRGALIAARFGRPELAARWAAIAADERDIVLRRGFNPQIGCFTQALDGEHGD
                     ASNLLFPSIGLLDARDPRFRATLDHYGRHMTRRGLLQRYTNLDDFGATTSAFTICSFW
                     WAEALALAGRLDEAVDVFHRVISHANPLGLFSEDIEPETGRLLGNFPQAYTHVGLIHA
                     AMTIGALLDVRDGRVRAWI"
BASE COUNT          356 a          816 c          848 g          325 t
ORIGIN      
        1 gctcttcacc ttgcggatcg agcccgacga agtcctcgcg gtagcgcccg ccgaccacct
       61 tggtgacccg gaacgtctgt tcattgcccc ggtcgatgcc cctggcggtg accacgtgca
      121 tgatccgctc gccgccttgc atcgccatcg tcacggtgtc gcccgcgacc cgcagggtgg
      181 cgccatcgaa cggcgcccgg atctgcgcgg tccgcgcatc gcgggtcatc ccgcgcatcg
      241 ccgaggcctc ccagcgggcg tagatctcgc cgtggcagtc cgcgcacgcc cgcgatccgg
      301 cgtagtcggt gcgcaggatg ttgctcgcga ccggcgggcc cggggcctcg cccaccgccc
      361 ccggcggcgc cgccggcgtc acgtgcggcg atcggcaggc gaccagcgcg gtgagccaga
      421 tccagacccg cacgcggtgg atgctagcac ctcgcgcgcg gcgccgcgcc cccgctacgc
      481 gctgcggtaa tagtgatggc gctcgcgcag accctcggcg gacagcgcgg cctgcacggt
      541 gggccggtcg gcgatccgcg cctcgtagtc atcgaggttg ggatacaggc cgaggtcgat
      601 cccctggggc gcgcaccact gcaggatcgt gaacaggtag gcgtcggcga cggtgaacgt
      661 ctcgcccatc aggaacgcgc ggtcctcgag cacgtcctgc aggtagagca gccgccggcc
      721 gatcttgccc cgcaggtgct cggcgaccgg cgcggagcgc gggaacgtcg ggtcgagcag
      781 cggcgcgaac tgcttgtgca gctcggtcga caggaagttg agccactcct gcaggtggta
      841 gcgtgcgaac gtcccgctgg gcggcacgag gcgggcgtcc ggtgcgagat cggcgatgta
      901 ctgcacgatc gccggcccct cggtgaggat cggagagccc ggcccgtcga gctgcagggc
      961 gggcacgtag cccttggggt tgaccgtgag gtagtccaga ccgctcgctg tgcggtggct
     1021 cttcagatcg acacgctcca gatcgaacct gcgctcggcc tcgcgcagca cgatgtgcgg
     1081 agacagcgag cacgcgcccg gggaatagta cagcttcatg tcggcctcct gaccacaata
     1141 cccagcaccc ggcgtgccgc aggaacaccc cgagatctgg tttgccaggc gggcgcatgg
     1201 tcacggccgt gcggatgccg gacacgcagc ggtgctgaac ccaccgaact tacgtgccac
     1261 cacgcaggcg cgcgatggca cgccgatcgc acttcgccgg tccaggcacg accaggatga
     1321 ggcacacgcc ggaacatcga ccggccgcgc agcgatcgtg gcgacacgat gcaatggctc
     1381 ccgatggcga ggtcacgcgg aggcatgcac ctggtccgtg ccaacctctt ccccggaccg
     1441 gtccgcgcga tatgatgtca gatccaggcg cgaacccggc cgtcgcggac gtcgagcagc
     1501 gcgccgatcg tcatcgcggc gtggatcagg ccgacgtggg tgtaggcctg cgggaaattg
     1561 ccgagcagcc gcccggtctc gggctcgatg tcctcggaga acagcccgag cgggttggcg
     1621 tgcgagatca cgcggtggaa cacgtcgacc gcctcgtcga gccggccggc gagcgcgagc
     1681 gcctcggccc accagaacga gcagatcgtg aacgccgagg tcgtcgcgcc gaagtcgtcg
     1741 aggttggtgt agcgctggag caggccgcgc cgggtcatgt gccggccgta gtgatcgagc
     1801 gtggcgcgga accgcggatc gcgggcgtcg agcaggccga tcgacgggaa cagcaggttg
     1861 gacgcgtcgc cgtgctcgcc gtcgagcgcc tgggtgaagc agccgatctg cgggttgaag
     1921 ccgcggcgca gcacgatgtc gcgctcgtcg gccgcgatcg ccgcccaccg cgcggcgagc
     1981 tcgggccggc cgaaccgcgc cgcgatcagc gcgccgcggt cgatcgccgc ccagcacatc
     2041 gcgcgcgaga acgtggagtg gcgcggcagc gtgcggaact cccagatccc ggtgtcggcg
     2101 agcggcgcca gcgcgatcgc ctgctcgacc agccgctcga tcagcgggaa gaacgtctcc
     2161 ggctgatcga tcacgatccg cggatcggac agcacggtct gcaggcacag gatcagctcg
     2221 ccgttgaggt cgttctgctg ctgctcggcg gcggcgttgc cgatccggac cggcccggtg
     2281 ccggcgaacc cggtgaggtg gtcgagcacc cgctcgggca gctcgcgctc gccgccgatg
     2341 ccgta
//
LOCUS       DASVOR010002030         1855 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_255805,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002030 DASVOR010000000
VERSION     DASVOR010002030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255805"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            325..1191
                     /locus_tag="VF516_37670"
     CDS             325..1191
                     /locus_tag="VF516_37670"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113539.1"
                     /translation="MSDGAVVPLAPPAHRLSQFGDYDIVTPLARGGMGGVYLAVHTWT
                     GERVALKVLDLQYAGHPEIVERLLAEHALASRVCHPGIVDIRGSYWTDDRVPYLVMEY
                     LAGETLGAIAERGPLELPAILSICAQAAAALAALHEAGVIHCDVKHDNLFVVGGDRPR
                     VKVIDFGVSRLVDEPPVDEPSVAGTPWCIAPEQWRGQPCPASDVYALGCVLYDLTTGH
                     PPFDGSLPELMTAHLECRPTRPSWVRPMPIELERLILRSLAKRPDDRPAMHEVAVALG
                     ELADQVAERAAG"
     gene            1248..>1855
                     /locus_tag="VF516_37675"
     CDS             1248..>1855
                     /locus_tag="VF516_37675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113540.1"
                     /translation="MTSAGDYVAMCKALLDNVRWFDASPVGSPAGSPFDADQLAAVVR
                     AQQAQLPACYRTAYAAPLLAGLPDLVAQLRQQVDRCLALGVASTDAIADVRAITDALV
                     GAVRDRGEVAYHAPLTRFQAVVSNLYRSFLAREQRVRLPQPLAEILPPLVTFAPDTER
                     GPFTLPVDRVRQFIGASVGVVSLPGAYAAHPLVWAGLAHETGG"
BASE COUNT          224 a          706 c          655 g          270 t
ORIGIN      
        1 cgccacgacg cggcgcgggt cgccgcgctc gccggcaagc tcgccggcga tccaaagcgg
       61 cgcgcgacgc tggtctgcgc cggcgccggc atcgatttgc caagatgaca gcggcgcacg
      121 gccggtcgtt gcctttccat acagcgggtg tcggcctttg cccgccgttt gcccgccgcg
      181 gcgccaatat attcacagtg gtcgccgccg gcacagcctg ccccacgccg gaacagccag
      241 ctacgcgcgg aactgccgtg tttcgcacat ggcatcgaca atgcaactcg cggccacatg
      301 gtcaccgcga cgttgccgct gaggatgagc gacggcgccg tcgtgccgct cgcgccgccg
      361 gcgcaccggc tctcgcagtt cggcgactat gacatcgtga ccccgctggc aagaggtggc
      421 atgggcggcg tctacctcgc cgtacacacc tggaccggcg agcgggtcgc cttgaaggtc
      481 ctcgatctgc agtacgccgg ccatcccgag atcgtcgagc gcctgctggc cgagcacgcg
      541 ctcgcgtcgc gcgtctgcca ccccggcatc gtcgacatcc gcggctcgta ctggaccgac
      601 gaccgcgtcc cgtacctggt gatggagtac ctggccggtg agaccctggg cgcgatcgcc
      661 gagcgcggcc cgctggagct gccggcgatc ctctcgatct gcgcgcaggc cgccgcggcg
      721 ctggcggcgc tccacgaggc cggcgtgatc cactgcgacg tcaagcacga caacctgttc
      781 gtggtcggcg gcgaccggcc gcgggtcaag gtgatcgact tcggcgtgtc gcgcctggtc
      841 gacgagccgc cggtcgacga gccgtcggtc gccggcacgc cctggtgcat cgcgcccgag
      901 cagtggcgcg gccagccgtg cccggccagc gacgtgtacg cgctcggctg cgtgctctac
      961 gacctcacca ccggccaccc accgttcgac ggctcgctcc cggagctgat gaccgcgcat
     1021 ctcgagtgcc ggccgacccg gccgtcgtgg gtgcggccga tgccgatcga gctcgagcgg
     1081 ctgatcctgc gctcgctcgc caagcgcccc gatgatcggc ccgcgatgca cgaggtcgcg
     1141 gtggcgctcg gcgagctcgc cgaccaggtc gccgagcgcg ccgccggctg agcgcgcttg
     1201 tcggcggcgc cggccgggcg tagtgtctcc cgggcacgca accggcgatg acgagcgcgg
     1261 gcgactacgt ggcgatgtgc aaggcgttgc tcgacaacgt ccggtggttc gacgcgtcgc
     1321 cggtgggctc tccggccggc tcgccgttcg acgccgacca gctcgccgcc gtggtccggg
     1381 cgcagcaggc ccagctgccc gcgtgctatc gcaccgcgta tgcggcgccg ctgctcgccg
     1441 ggctgcccga cctcgtcgcg cagctccgcc agcaggtcga ccgctgcctg gcgctcggcg
     1501 tggcgtcgac cgacgcgatc gccgatgtcc gggcgatcac cgacgcgctg gtcggcgcgg
     1561 tccgcgatcg cggcgaggtc gcctaccacg cgccgctgac ccggttccag gccgtggtct
     1621 ccaacctcta ccgctcgttc ctcgcccgcg agcagcgcgt ccggctgccg cagccgctcg
     1681 ccgagatcct gccgccgctg gtgacgttcg cgccggacac cgagcgcggg ccgttcacgc
     1741 tgccggtcga ccgggtgcgc cagttcatcg gcgcatcggt cggcgtggtc agcctgcccg
     1801 gcgcctacgc ggcgcacccg ctggtgtggg ccggcctggc gcacgagacc ggcgg
//
LOCUS       DASVOR010002031         3699 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_489278,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002031 DASVOR010000000
VERSION     DASVOR010002031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489278"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2580
                     /locus_tag="VF516_37680"
     CDS             <1..2580
                     /locus_tag="VF516_37680"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8113541.1"
                     /translation="PAPAVRAHATRRTGAIAVDGRLDEAAWASAPKHSGFIQRFPKDA
                     TPPSVDTRFAIVYDDDAVYVGVWADDPRPDLIRALLTRRDVDAPADAITVAFDSYHDR
                     RTAYAFQLNAAGVQRDMLLFDDTNSDDTWDAVWTGNVAVTATGWTAEYRIPLSQLRFA
                     SADRQEWGFQILRVIGRTGEQDTWSPWPRSAPQVVSKFGVVDGITHLGDKTRLELLPY
                     ATTGVDRMPVDAGDPLNRTYNWRRGIGLDLKYGLGPAFTLSATINPDFGQVEADPSKI
                     NLSGSELFFAEKRPFFLEGVDLFKLQIGNSDGSPEGAFYSRRIGAAPPTPDARYDFIK
                     APTSTAIYGAAKLTGKTASGWSVGVLDAVTGRETTEIATAADATGAGGSRSDLASAPL
                     TNYAIARVKRDLNDGATSIGASATAVNRQLDDPRLAALDHDQAYTGGLQLQHRWADNA
                     WTANVNLLGSWVHGSPEAIAATQKDAVHYFQRPDDRAASFDPTRTSLSGLAAKYMVGM
                     LGDTQHWRFGAGGDLRTPGLELNDAGFQLNSDRAIPFFWAQYREDKPSEHLLNWQVSS
                     DVFWVQTFEPRLADYGLETNANVQFANYWTMGAYTNLARGKWFINALRGGPALRGDPT
                     TFANAFLNTDTRAPVWFSINAYGGHNWVSDERNGGIDLGATIQARSNLDLSIGPSIYH
                     RNEPMQYIDQVGDDVMADQTHFVFGRLHETDVSMTVRANWTFSPHLALQVYAQPYIAS
                     GYYTELKEIDHPGAAHYRDRFHVLTGDELALSDGTYHVTRGGASYSVSQPDFNFRQLR
                     STLVLRWEYRPGSTVFAIWSHGRTSDTFDDGRFGFGHDLSELAAARSENIVMVKANYW
                     IGL"
     gene            2599..>3699
                     /locus_tag="VF516_37685"
     CDS             2599..>3699
                     /locus_tag="VF516_37685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113542.1"
                     /translation="MESLPATAAANQFGKYSLVARLATGGMAEIFLARLQGAAGFEKL
                     VCIKRILPHLARDRQFVAMFLDEARIAARITHPNVCQVFELGEIAGSYYLAMEYLEGI
                     PLACFRRDDDYGSEPDPRLVAGIAVQACEGLHHAHQLRHSDGSVMEVVHRDMSPQNLF
                     VTADGIVKVLDFGIAKIQGATVRTSTGAIKGTYAYMAPEQLRGERVDRRTDVFALGIV
                     MWETLARRTLFKRDTEFLTFQAITAEPIEDICAIRPDVPAALSSVIMTALARDRDERF
                     PTARMLGEAIAAAVQPLTSSAISEEIQRAFPDELADQAELIRVAREGGVFDLDVERGP
                     SIGHGADLLTTPISRQRPAAKTVSDGPRRVTGG"
BASE COUNT          589 a         1358 c         1247 g          505 t
ORIGIN      
        1 cccgcaccgg cggtgcgcgc ccacgcgacc cgccgcaccg gcgcgatcgc ggtcgacggc
       61 cggctcgacg aggcggcgtg ggcgagcgcg ccgaagcaca gcgggttcat ccagcgcttc
      121 cccaaggacg cgaccccgcc gtcggtcgac acccggttcg cgatcgtcta cgacgacgac
      181 gcggtctacg tcggggtgtg ggccgacgat ccgcggcccg atctgatccg cgcgctgctc
      241 acccggcgcg acgtcgacgc cccggccgac gcgatcacgg tcgcgttcga cagctaccac
      301 gaccgccgga ccgcctacgc gttccagctc aacgccgccg gcgtccagcg cgacatgctg
      361 ctgttcgacg acaccaactc cgacgacacc tgggacgcgg tgtggaccgg caacgtcgcg
      421 gtgaccgcga ccgggtggac ggccgagtac cggatcccgc tcagccagct gcggttcgcc
      481 agcgccgacc gccaggagtg ggggttccag atcctgcggg tgatcggccg gaccggcgag
      541 caggacacct ggtcgccgtg gccgcgcagc gcgccccagg tggtcagcaa gttcggcgtg
      601 gtcgacggca tcacccacct gggcgacaag acccggctcg agctcttgcc gtacgcgacc
      661 accggcgtcg atcgcatgcc ggtcgacgcg ggcgatccgc tcaaccggac ctacaactgg
      721 cggcgcggca tcggcctcga tctcaagtac ggcctgggcc cggcgttcac gctgtcggcg
      781 acgatcaacc ccgacttcgg ccaggtcgag gccgacccgt cgaagatcaa cctgtcgggc
      841 agcgagctgt tcttcgccga gaagcggccg ttcttcctcg agggcgtcga tctgttcaag
      901 ctgcagatcg gcaacagcga tggtagcccc gagggcgcgt tctacagccg gcggatcggg
      961 gcggcgccgc cgacgcccga cgcccgctac gacttcatca aggccccgac gtccacggcg
     1021 atctacggcg cggccaagct gaccggcaag accgcgagcg gctggtcggt cggcgtgctc
     1081 gacgcggtca ccggccgcga gaccaccgag atcgcgaccg cggccgacgc gaccggcgcc
     1141 ggcggctcgc ggtccgacct cgcatcggcg ccgctgacca actacgcgat cgcccgggtc
     1201 aagcgcgacc tcaacgacgg cgccacgtcg atcggcgcgt cggcgaccgc ggtgaaccgc
     1261 cagctcgacg atccgcggct cgccgcgctc gaccacgatc aggcctacac cggcggcctc
     1321 cagctccagc accgctgggc cgacaacgca tggaccgcga acgtcaacct gctcggcagc
     1381 tgggtccacg gcagccccga ggcgatcgcc gcgacccaga aggacgcggt ccactacttc
     1441 cagcgccccg acgaccgcgc cgcgagcttc gacccgacgc gcaccagcct gtccgggctg
     1501 gccgccaagt acatggtcgg catgctcggc gacacccagc actggcggtt cggcgccggc
     1561 ggcgatctgc ggaccccggg gctcgagctc aacgacgccg ggttccagct caactccgat
     1621 cgcgcgatcc cgttcttctg ggcccagtac cgcgaggaca agccgagcga gcacctgctc
     1681 aactggcagg tcagctccga cgtgttctgg gtccagacct tcgagccgcg gctcgccgac
     1741 tacggcctgg agaccaacgc caacgtccag ttcgcgaact actggacgat gggcgcgtac
     1801 accaacctcg cgcgcggcaa gtggttcatc aacgcgctgc gcggcgggcc cgcgctgcgc
     1861 ggcgatccca cgacgttcgc caacgccttc ctcaacaccg acacccgcgc gccggtgtgg
     1921 ttcagcatca acgcctacgg cggccacaac tgggtctcgg acgagcgcaa cggcggcatc
     1981 gatctcggcg cgacgatcca ggcccggtcc aacctcgatc tgtccatcgg cccgagcatc
     2041 taccaccgca acgagccgat gcagtacatc gaccaggtcg gcgacgacgt catggcggac
     2101 cagacccact tcgtgttcgg ccggctgcac gagaccgacg tgtcgatgac ggtgcgggcg
     2161 aactggacgt tctcgccgca cctcgcgctg caggtctatg cccagcccta catcgcgtcg
     2221 ggctactaca ccgagctcaa ggagatcgac cacccgggcg cggcgcacta ccgcgaccgc
     2281 tttcacgtgc tgaccgggga cgagctggcg ctgagcgacg gcacctacca cgtcacgcgc
     2341 ggcggggcca gttacagcgt cagccagccc gacttcaact tccgccagct gcgatcgacc
     2401 ttggtcctgc gctgggagta ccggccgggc tcgacggtgt tcgcgatctg gagccacggc
     2461 cggaccagtg acacgttcga cgacggccgg ttcggcttcg gtcacgacct gtcggagctg
     2521 gcggccgcgc ggagcgaaaa catcgtgatg gtcaaggcaa attactggat cggcttgtag
     2581 gcgcgctatg ctcgcgtgat ggagtcgctg ccggcgacgg ccgcagccaa tcagttcggg
     2641 aagtacagcc tggtcgcccg gctcgcgacc ggcggcatgg ccgagatctt cctggcgcgc
     2701 ctgcagggcg ccgccgggtt cgagaagctg gtgtgcatca agcggatcct gccgcacctc
     2761 gcgcgcgatc ggcagttcgt cgcgatgttc ctcgacgagg cgcgcatcgc ggcgcggatc
     2821 acgcacccca acgtgtgcca ggtgttcgag ctcggcgaga tcgcgggcag ctactacctg
     2881 gcgatggagt acctcgaggg catcccgctg gcctgcttcc ggcgcgacga cgactacggt
     2941 tcggagccgg atccccggct ggtcgccggc atcgcggtcc aggcctgcga gggcttgcac
     3001 cacgcgcacc agctccggca cagcgacggc agcgtgatgg aggtcgtcca ccgcgacatg
     3061 tcgccgcaga acctgttcgt caccgccgac ggcatcgtca aggtgctcga cttcggcatc
     3121 gccaagatcc agggcgcgac ggtgcggacc agcacgggcg cgatcaaggg cacctacgcg
     3181 tacatggcgc ccgagcagct gcgcggcgag cgcgtcgatc gccgcaccga cgtgttcgcg
     3241 ctcggcatcg tgatgtggga gaccctggcc cgccgcaccc tgttcaagcg cgacaccgag
     3301 ttcctgacgt tccaggcgat caccgccgag ccgatcgagg acatctgcgc gatccggccc
     3361 gacgtgccgg cggcgctgtc gtcggtgatc atgaccgcgc tggcgcgcga ccgcgacgag
     3421 cggttcccga ccgcgcggat gctcggcgag gcgatcgccg ccgcggtcca gccgctgacc
     3481 tcctcggcga tcagcgagga gatccagcgc gcgttccccg acgagctcgc cgaccaggcc
     3541 gagctgatcc gggtcgcgcg cgagggcggc gtgttcgatc tcgacgtcga gcgcgggccg
     3601 tcgatcggtc acggcgccga cctgctcacc acgccgatct ccaggcagcg cccggcggcg
     3661 aagacggtca gcgacgggcc gcggcgggtg accggtggg
//
LOCUS       DASVOR010002032         3495 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_226242,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002032 DASVOR010000000
VERSION     DASVOR010002032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226242"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF516_37690"
     CDS             complement(<1..630)
                     /locus_tag="VF516_37690"
                     /inference="COORDINATES: protein motif:HMM:NF036997.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113543.1"
                     /translation="MDRVPYGERLGLALASADGEMFPARAEEGAPAWLREQAVPRPPH
                     AAGLAPEPGEAPPGTERGRSLSLPRSTSREADRAAAGSAGAGAQPGTAARFAEVAVTL
                     PVPGRFHYAIPEPLAPRAQVGARVLVRFGARKVTGVVVRIDAAPPPGIAPIALSDVLD
                     EVPALSPELVELCAWIADYYEAPPGEVIRAALPAGSGVAARSVFALTPAG"
     gene            complement(1141..2028)
                     /gene="galU"
                     /locus_tag="VF516_37695"
     CDS             complement(1141..2028)
                     /gene="galU"
                     /locus_tag="VF516_37695"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEX8113544.1"
                     /translation="MTIRSNVRKAVIPAAGLGTRFLPATKAVPKEMLPIVDIPTIQLV
                     IDEAVRAGVTDVIVVNGRNKHAIEDHFDHAYELEHTLRERGNTRLADEMAQVSRLVRL
                     ISVRQKHPLGLGHAVLCAREAVGDEPFAVLLGDDLIDNDTDPGIGQLMRVHGETGAAA
                     IAIMEVPAGKEHMYGIVTGDLDASGRMRIRDMIEKPKPGTAPSRWAVVGRYVLPATVW
                     PHLAATRPGAGGEIQLTDALRELIGSPEGLYGVVLTGTRHDAGDRLGYLRANLAYALK
                     RPELREAVIALCREMLGPA"
     gene            2079..>3495
                     /locus_tag="VF516_37700"
     CDS             2079..>3495
                     /locus_tag="VF516_37700"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8113545.1"
                     /translation="MADAEITETLRRSVVKLFTVLKEPNYYQPWDMEYQKTSGGSACI
                     VPGNRILTNAHVVSHHLYIQALKPGDPRKYTARVLCVDHDTETALLTVDDPQFFEGTI
                     PVRFGELPFRNARVAVYGFPVGGNELCVTAGIVSRIEVRAYTHSQRHLLALQTDAAIN
                     PGNSGGPVFMNDELIGIAFQSYKQKDLEKAGYVVPIPIIRHLFDDLEDGAITGVPDLG
                     IYWQKIENTSLRDYVGLTGGRTGVLITRVLHGASADGVLHEGDVLTAIDGVPVASDGS
                     IPLRAHDRVHFSYLVSRHQVGAQLTVEILRRGELHRLTVQLRKLASLVPPPQPALRPT
                     YFIFAGLVFLPLTYDYMATWKWDNVAPRFKHYYYSRLPSERHEQIVLVNQVLAHDVNV
                     GYHQVHGAVVERINGIDIVSMRDIPRALETPLGNFHVIELDYHGTRGESSDYHASYGT
                     RIVLDASTAARATAEILAQNGI"
BASE COUNT          550 a         1301 c         1134 g          510 t
ORIGIN      
        1 ccccgccggg gtcagcgcga acacgctgcg cgcggcgacc ccgctgccgg ccggcagcgc
       61 cgcgcggatc acctcgcccg gcggcgcctc gtagtagtcg gcgatccacg cgcacagctc
      121 gaccagctcg ggcgacagcg ccggcacctc gtcgagcacg tccgacagcg cgatcggcgc
      181 gatgcccggc gggggcgcgg cgtcgatccg caccacgaca ccggtcacct tgcgggcccc
      241 gaaccggacc agcacgcgcg ccccgacctg cgcgcgcggt gcgagcggct ccggaatggc
      301 gtagtgaaac cgccccggca cgggcagggt caccgcgacc tcggcgaacc gcgccgcggt
      361 gccgggctgc gccccagcgc ccgcgctccc ggccgccgcg cgatccgcct cccgcgacgt
      421 gctcctgggc agcgagagcg agcggccgcg ctccgtgcca ggcggagcct cgccaggctc
      481 tggggccaac ccggccgcat gcggagggcg cggcacggcc tgctctcgca gccacgccgg
      541 agcgccctcc tctgcccgcg ccgggaacat ctccccgtcg gcgctggcga gcgccagacc
      601 cagtcgctcg ccgtatggca cgcggtccac gcccacatcc tgcaccggct acctgacgac
      661 cggcgggcaa tcgggcacgt ccagacggcg ctggattcag aattcaccag tgatcttgaa
      721 gtattgcaga aaatagcgca gtgcgccgat ccccgggatc tggcctcgat gcgaggcttc
      781 gaggcgcttt gccctgcata tgctccctcg acgatgacgt tgaccttgac tcgagcgtcg
      841 agcttgacgg cagcgaagcg atacgtagac gtctgacccc accgtcgacc tcgacctcgc
      901 ccccgtgccc gtggtcgcgc tccaccgaag cgggtcgcgc aggagcgccc gacgtccagg
      961 gtcgagggtg ggctcaacgc cgtacgtcgc cgtcgacgtc aacgcttcgg gtcaacgacc
     1021 aggtcgacgt cgacgatcac gaacgccaac tcgagctctc gtgacgaatc atccgtcgtc
     1081 gaggtcgacg tcgcacgggc cacgaacgcg caacttgtga tgaagcatca gacatacgcc
     1141 tcaggccggt ccgagcatct cgcggcacag cgcgatcacg gcctcgcgca gctccggccg
     1201 cttcagcgca tacgcgaggt tggcgcgcag gtagccgagc cggtcgccgg catcgtggcg
     1261 cgtgccggtg agcaccacgc cgtacaggcc ctcgggcgat ccgatgagct cgcgcagcgc
     1321 atcggtcagc tggatctcgc cgccggcgcc gggccgggtc gccgccaggt gcggccacac
     1381 cgtcgccggc agcacgtagc gcccgacgac cgcccaccgg ctcggcgcag tgcccggctt
     1441 gggcttctcg atcatgtcgc ggatccgcat ccgaccgctc gcgtcgaggt cgccggtgac
     1501 gatgccgtac atgtgctcct tgccggcggg gacctccatg atcgcgatcg ccgccgcgcc
     1561 ggtctcgccg tgcacgcgca tcagctggcc gatcccagga tcggtgtcgt tgtcgatcag
     1621 gtcatccccg agcaggacgg cgaacggctc gtcgccgacc gcctcgcgcg cgcacagcac
     1681 cgcgtggccg agccccagcg gatgtttctg gcggaccgag atcagccgga ccagccgcga
     1741 gacctgtgcc atctcgtcgg cgagccgggt gttgccgcgc tcgcgcagcg tgtgctccag
     1801 ctcgtacgcg tgatcgaagt gatcctcgat cgcgtgcttg ttgcgcccgt tgaccacgat
     1861 gacgtcggtg accccggcgc ggaccgcctc gtcgatcacc agctggatcg tcggaatatc
     1921 gacgatcggc agcatctcct tgggcaccgc cttggtcgca ggaagaaatc gggtgccgag
     1981 ccccgccgcg gggatcacgg cttttcgtac gttggaccga atcgtcatcg gcgctcgacc
     2041 ttaccatcgc cgggctgaaa ccgctgtagc ctggtggcat ggcagatgcc gagatcaccg
     2101 agacgctgcg gcgctcggtc gtcaagttgt tcaccgtgct caaggagccc aactactacc
     2161 aaccgtggga catggagtac cagaagacct ccggaggatc ggcgtgcatc gtcccgggga
     2221 accggatcct gaccaacgcc cacgtcgtgt cacatcacct ctacatccag gcgctcaagc
     2281 cgggcgatcc gcgcaagtac accgcgcggg tgctgtgcgt cgatcacgac accgagaccg
     2341 cgctgctcac cgtcgacgat ccccagttct tcgaggggac gatcccggtg cggttcggcg
     2401 agctgccgtt ccgcaacgcg cgggtcgcgg tctacggctt cccggtcggc ggcaacgagc
     2461 tgtgcgtcac cgccggcatc gtgtcgcgca tcgaggtccg cgcctacacc cactcgcagc
     2521 gccacctgct cgcgctgcag accgacgccg cgatcaaccc cggcaacagc ggcggcccgg
     2581 tgttcatgaa cgacgagctg atcgggatcg cgttccagtc ctacaagcag aaggacctcg
     2641 agaaggccgg ctacgtcgtc ccgatcccga tcatccgaca cctgttcgac gacctcgagg
     2701 acggcgcgat caccggcgtc ccggacctcg ggatctactg gcagaagatc gagaacacca
     2761 gcctgcgcga ctacgtcggc ctgaccggcg ggcgcaccgg cgtgctgatc acgcgcgtcc
     2821 tgcacggcgc gtcggccgac ggcgtgctcc acgagggtga cgtgctcacc gcgatcgacg
     2881 gcgtgccggt ggcgtccgac ggctcgatcc cgctgcgcgc gcacgaccgc gtccacttct
     2941 cgtacctggt cagccgccac caggtcggcg cgcagctcac ggtcgagatc ctgcgccgcg
     3001 gcgagctgca ccggctcacc gtccagctcc gcaagctggc ctcgctcgtg ccgccgcccc
     3061 agcccgccct gcggccgacc tacttcatct tcgccgggct ggtgttcctg ccgttgacct
     3121 acgactacat ggcgacgtgg aagtgggaca acgtcgcgcc gcggttcaag cactactact
     3181 acagccggct gccgtcggag cgccacgagc agatcgtcct ggtcaaccag gtcctcgccc
     3241 acgacgtcaa cgtcggctac caccaggtcc acggcgcggt ggtcgagcgg atcaacggca
     3301 tcgacatcgt gtcgatgcgc gatatcccgc gcgcgctcga gacgccgctc ggcaacttcc
     3361 acgtgatcga gctcgactac catggcacgc gcggcgagtc gtccgactac cacgcgagct
     3421 acggcacccg gatcgtgctc gacgccagca ccgcggcgcg cgcgaccgcc gagatcctgg
     3481 cccagaacgg gatct
//
LOCUS       DASVOR010002033         7947 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_342528,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002033 DASVOR010000000
VERSION     DASVOR010002033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7947
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342528"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1806)
                     /locus_tag="VF516_37705"
     CDS             complement(163..1806)
                     /locus_tag="VF516_37705"
                     /inference="COORDINATES: protein motif:HMM:NF017733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HEX8113546.1"
                     /translation="MSDHFYDDFLAELKAVDDFLAYRTGDATSVAREDPDVRRLMESI
                     AFFSVRTRQAASDQLRRSVLSLTRGHLDDFLSPQPARGLVQAVAAGLVTDTAYLPAGT
                     LLRLTTADGDLGLFSTMTDVTLRPLQIDLAELGLRPGGGFRLVIRIRARREVVDVPEP
                     LAIHLSYLGDYAPSLRFQYRLRRHLQGGGLSVFYDAAPDPARTGAPCEVRFGSAPHAQ
                     AAAGSGSPIEQLRSFLHFPARELYLQISLPRPAAPWRQAWLCLDLDDQWPSELVVNKD
                     VFRLFVLPIENLVRDVALPIKCDGTRVHYPIVPARADAKLVLHSVTGVFQELPSGPDP
                     ILPVQLASGDQSYDIEHVAEGPGGVEPRLVLRLPDAFAAPRLISVEARWYQPGFDRVA
                     VGKLEARLQTRHIQGVELRPLGVLSPHRQSALWRDPAAMLQVLSRRVSRVLGRRDLIS
                     LMTLLGADADSHHAGIDADLLHIEVREEPADLRRGGGIRHVYQITIGDVDEDRLGLVA
                     DYLRCVQTLLDAWSANPVRLEVFRRTSGARRLPMIEGGS"
     gene            complement(1811..2383)
                     /locus_tag="VF516_37710"
     CDS             complement(1811..2383)
                     /locus_tag="VF516_37710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113547.1"
                     /translation="MAKLVCTVEMSKDAGITIKVENADASITQTITMDGTTLTMKVAG
                     SDHTSTFTQKADSIAIKCKDFSIEADETITCTSGKATRHHSDDTLTLESTKDMTLTSS
                     AKLVQSADSDVTVSGANVKATAQSAASVKGMTAELAGDQSLTLKGTQASLSGAQVAIK
                     ADAQLGAESSGIATLGGNMTTIKGTLINAG"
     gene            complement(2388..4076)
                     /locus_tag="VF516_37715"
     CDS             complement(2388..4076)
                     /locus_tag="VF516_37715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113548.1"
                     /translation="MTAQALDLAQQGAGGGDRFADRLAVTLTLSIGGTPHAVPGGAVK
                     ELALDLRSYGFSGSLAFLVQDDQAHGGGFSDELRAGFLKPDLIEVSLTVAPVLVAPEA
                     GPIQPITLKGLVTDKSVSEVLFRKTADLPVLVRRYRIVFADPAQVLWGQHYPCQLYTD
                     KSMVDVINDHLGDKITARYDFSALAQSRRMVFLHLPRGAGASFWDFLSWYLDGCAGVL
                     SYDYAAAEYVVAAAKSETGTPIQLFGDDLGSAELYYPEVPRHAPVVLNSYADSPRTEP
                     VSSAQAQTGIRSDLLLRTPIAQDVDDAVAFQRTRTAPPCCEAHLAFARMPSDTLVPGA
                     LVELPAANRWSSESSLVGVTWRVRRLRVRAVAPSGPLDRDLQETSTAYRVEVSAELED
                     KTDPRIDRPPFHAPSYPGLVEGKIVSEQGQDGEKTYQVYRDTTTSLDEYHVKVPLWSD
                     QIVSAPLEPILGSGNLYWPSYRDERVLLALDVHGAQIARLLEWRTGAPLSMEAQGEHL
                     LFGKSDTSQTSVNHVYDGDNPVFNVARTHQSDTALIQLREGTLFLQVKEQEQGS"
     gene            complement(4073..4474)
                     /locus_tag="VF516_37720"
     CDS             complement(4073..4474)
                     /locus_tag="VF516_37720"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX8113549.1"
                     /translation="MADPTRRRQAFLGRLAQLLPERAPVEIARNLGFVFNTRKACGSV
                     IADFGLGDYEREVNTGRAVEALRVELLAAVSRHEPRIREPRVRLLGGWHTNLVRFEIA
                     GRVDGEACVLDVDIDTTTRHVTVQVAEAGRR"
     gene            complement(4467..5828)
                     /gene="tssK"
                     /locus_tag="VF516_37725"
     CDS             complement(4467..5828)
                     /gene="tssK"
                     /locus_tag="VF516_37725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HEX8113550.1"
                     /translation="MDPHTSQDVLAARDWQPRPSYDRLALHRWEMGQVLHPAQFRAQE
                     EALLEHVGLRVQLTGLPSRGVALLRWGDAQLASGDLSISALTVVLPSGRLIDVPGNAV
                     LTPRKLVLPAKAAGPVPVYLHLRGELRPDGARDLPRYEADEPEIRRAVYQVEISAEPG
                     IESEQERMKLAELSFSDGAWALAPYVPPLIRLGSGTTPFLRDTMAQIAAALGGFESEL
                     VRRAADSATGAESMSEVRRILTALYRVQAVLSDHGYGAATQAVALHPYHLFAALRDFY
                     LEAVVLQGKPLEDWPPRYQHDDLRGCFERLARGLATTLSVPAVVSPRLAFQRDEHWFI
                     TGAFPDELCWAREVFLLLERASEAPGAERTAPPALDDLKLASPLRAEEVYTKALEGVP
                     RRAIPSLGFAQTFGHRATVFQLDAASPEWRQAVVERALCFAVLPGLENTHAALFWQSP
                     DHG"
     gene            complement(5860..6435)
                     /locus_tag="VF516_37730"
     CDS             complement(5860..6435)
                     /locus_tag="VF516_37730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113551.1"
                     /translation="MPTFQKSCDIKQGYNFKKDIQTPVGYLTSLKIGDVTLKADQTVK
                     DPMSPETDFPVVAVLSGAMWELGVTDTLYYTGQISVYNKQQTQLLVYKDLSKVDVVFK
                     FSVYSYDPIEKKYFKCMLPATDSDLNGLLEKNGADLNLSVADDASGEVQSPENYAFQI
                     GIKPQPSAQTVTVATSFSDKVVKSWGMKVEK"
     gene            complement(6467..>7947)
                     /gene="tssC"
                     /locus_tag="VF516_37735"
     CDS             complement(6467..>7947)
                     /gene="tssC"
                     /locus_tag="VF516_37735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HEX8113552.1"
                     /translation="GATTAPGAPPAAAGATPAAPAIVNLQFRDQDEDFPYEDRFINSL
                     GAFLVNIDLAQVTDIRRVMMEIDRLSVSLDTAIQDQIQQVLHSPSFQRLEASWRSLQD
                     ICSEVNSNEIIVDFLDIDKAALGQDLQDHSAYILNSALFRKVYVEEYDRYGGRPFGVM
                     IGLYDFDSTEEDIEWLETMSHISAAAHCPFVAAARPQFFGVDSWAELERKDDLETLLS
                     MPQFGRWDAFRSTDHAAYIGLTLPRYMLRAPYDQNTGKSKFVTFVEKIPNPETDYLWG
                     NAAVLFARNMVRSFEDSGWCQHIVGPRGGGLIRGLPVDMVMNHGHLELQPPVEVAIPD
                     YRELQFANTGFMALIHCKGTADATFFSARAVKKPIEFEEDLATKNADLVCYLDYTLSI
                     TRIAHYVKRMVRDYIGSTADAPYIQSMLEAWLIEYVTTAANPDDLTLLYYPFKAMSVS
                     VVPKPGPFGWYSCTVSVLPHIKFQGMDVELRLEAALGGAAAA"
BASE COUNT         1154 a         2745 c         2837 g         1211 t
ORIGIN      
        1 ccgccccccg caccagctgg gggccgctcg gcggacgcgg gggggtccgc cgcacgggcg
       61 ccgggcgcgg cggggtctgg ttcggatagt cgccgacggt cgaggcgacc gcccgatcga
      121 cgtcgccgcg cgtcgcgagg atcgcgcgcc acagctcgta cctcatgatc ccccctcgat
      181 catcggcagc cggcgcgcac cggacgtccg ccggaacacc tccagccgga ccgggttcgc
      241 gctccaggcg tcgagcaggg tctggacgca gcgcaggtag tccgcgacca ggccgagccg
      301 gtcctcgtcg acgtcgccga tcgtgatctg gtagacgtgg cggatcccgc cgccgcggcg
      361 caggtcggcg ggctcctcgc gcacctcgat gtggagcagg tcggcgtcga tgccggcgtg
      421 gtggctgtcg gcgtcggcgc cgagcagcgt catgaggctg atcagatcgc gccggcccag
      481 cacgcggctg acccggcgcg acagcacctg cagcatcgcc gccggatcgc gccacagcgc
      541 gctctggcgg tgcggcgaca gcacgcccag gggccgcagc tcgacgccct ggatgtgccg
      601 ggtctgcagg cgcgcctcga gcttgccgac cgcgacgcgg tcgaacccgg gctggtacca
      661 gcgcgcctcc accgagatca ggcgcggcgc ggcgaacgcg tcgggcagcc gcagcaccag
      721 ccgcggctcg accccgcccg ggccctcggc gacgtgctcg atgtcgtagc tctgatcgcc
      781 gctcgccagc tgcaccggca ggatcggatc gggccccgag ggcagctcct ggaacacgcc
      841 ggtcaccgag tgcaggacca gcttggcatc ggcgcgcgcc gggacgatcg gatagtgcac
      901 gcgcgtgccg tcacacttga tcggcagcgc cacgtcgcgc accaggttct cgatcggcag
      961 cacgaacagc cggaacacgt ccttgttgac caccagctcg gacggccact ggtcgtcgag
     1021 atcgaggcac agccacgcct ggcgccacgg cgcggccggc cgcggcagcg agatctggag
     1081 gtacagctcc ctggccggaa aatgcaggaa gctccgcagc tgctcgatcg ggctgcccga
     1141 gccggcggcg gcctgcgcgt gcggcgcgct gccgaacctg acctcgcacg gcgccccggt
     1201 gcgcgccgga tcgggcgccg cgtcgtagaa caccgagagc ccgccgccct gcaggtggcg
     1261 gcgcagccgg tactggaagc gcagcgacgg cgcgtagtcg cccaggtagc tcaggtggat
     1321 cgccagcggc tcggggacat cgaccacctc gcggcgcgcg cggatccgga tcaccagccg
     1381 gaacccgccg cccggtcgca gcccgagctc ggcgagatcg atctggagcg gccgcagtgt
     1441 cacgtcggtc atcgtcgaga acaggccgag atcgccgtcc gccgtggtca gccgcagcag
     1501 ggtccccgcc ggcaggtagg cggtgtcggt gaccagcccg gccgccacgg cctggaccag
     1561 cccgcgcgcc ggctgcggcg acaggaagtc gtcgaggtgg ccgcgcgtca gcgacagcac
     1621 cgagcggcgc agctggtcgc tcgctgcctg ccgggtccgc accgagaaga acgcgatcga
     1681 ctccatcagg cggcggacgt cgggatcctc gcgcgccacg ctcgtggcgt cgccggtgcg
     1741 gtaggccagg aagtcgtcca ccgccttgag ctcggccagg aagtcgtcgt agaagtgatc
     1801 gctcaccgca tcatcccgcg ttgatgaggg tgcccttgat ggtggtcatg ttaccgccca
     1861 gggtcgcgat tccggacgac tcggcgccga gctgcgcgtc ggccttgatc gccacctggg
     1921 cgccggacag gctggcctgg gtgcccttga gggtcagcga ttgatcgccg gcgagctccg
     1981 cggtcatccc cttgacgctg gccgcgctct gcgcggtggc cttgacgttg gcgccggaca
     2041 ccgtcacgtc cgagtccgcc gattgcacga gcttggcgct cgaggtcagg gtcatgtcct
     2101 tggtgctctc gagggtcagg gtgtcgtcgc tgtggtgcct ggtggccttg ccggaggtgc
     2161 acgtgatggt ctcgtcggcc tcgatcgaga agtccttgca cttgatcgcg atgctgtcgg
     2221 ccttctgcgt gaaggtgctg gtgtggtcgc tgcccgcgac cttcatggtc agcgtggtgc
     2281 cgtccatcgt gatggtctgg gtgatcgagg cgtcggcgtt ctcgaccttg atcgtgatgc
     2341 cggcgtcctt gctcatctcg accgtgcata ccagcttggc catcgcgcta gctcccctgc
     2401 tcctgctcct tgacctggag gaacagcgtc ccctcgcgga gctggatcag cgcggtgtcg
     2461 ctctggtggg tgcgcgccac attgaacacc gggttgtcgc cgtcgtagac gtggttgacc
     2521 gaggtctggc tggtgtcgga cttgccgaac aggaggtgct cgccctgggc ctccatcgac
     2581 agcggcgcgc cggtgcgcca ctcgagcagc cgcgcgatct gcgcgccgtg gacgtcgagg
     2641 gcgagcagca cgcgctcgtc gcggtacgac ggccagtaca ggttgccgga gcccaggatc
     2701 ggctcgagcg gcgcgctcac gatctggtcg ctccacagcg gcaccttcac gtggtactcg
     2761 tcgagcgagg tcgtggtatc gcggtacacc tggtaggtct tctcgccgtc ctggccctgc
     2821 tcgctcacga tcttgccctc gaccaggccg gggtagctcg gggcgtgaaa cggcggccgg
     2881 tcgatccgcg ggtcggtctt gtcctcgagc tcggcgctga cctccacccg gtacgccgtg
     2941 ctggtctcct ggagatcgcg atcgagcggc ccggacggcg ccaccgcgcg gacgcgcagc
     3001 cggcggaccc gccaggtcac gccgaccagc gagctctcgc tgctccagcg gttggccgcc
     3061 ggcagctcga ccagcgcgcc cggcacgagc gtgtccgacg gcatgcgcgc gaacgcgagg
     3121 tgcgcctcgc agcacggcgg cgcggtccgc gtgcgctgga acgcgacggc gtcgtccacg
     3181 tcctgggcga tcggcgtgcg cagcaggagg tcgctgcgga tcccggtctg cgcctgggcg
     3241 ctcgacaccg gctcggtgcg cgggctgtcg gcgtagctgt tcagcaccac cggcgcgtgg
     3301 cgcggcacct cggggtagta cagctcggcg gagccgaggt cgtcgccgaa cagctggatc
     3361 ggcgtgccgg tctcgctctt ggccgccgcc accacgtact ccgcggcggc gtaatcgtag
     3421 gacaaaacgc cggcgcagcc gtcgaggtac caggacagga agtcccagaa gctcgccccg
     3481 gcaccgcgcg gcaggtgcag gaacaccatc cgccggctct gggccagcgc gctgaagtcg
     3541 tagcgcgccg tgatcttgtc gccgaggtga tcgttgatca cgtcgaccat cgacttgtcg
     3601 gtgtagagct ggcaggggta gtgctgcccc cacagcacct gcgccgggtc ggcgaacacg
     3661 atccggtagc gccggaccag caccggcaga tcggcggtct tgcgaaacag cacctcggac
     3721 accgacttgt cggtcaccag acccttcagc gtgatcggct ggatcggccc ggcctcgggc
     3781 gccaccagca ccggcgccac cgtcagcgag acctcgatca gatccggctt gaggaagccg
     3841 gcgcggagct cgtcgctgaa cccgccgccg tgcgcctgat cgtcctggac caggaacgcc
     3901 agcgagccgg agaacccgta gctgcgcaga tcgagcgcca gctccttgac ggcgccgccg
     3961 gggaccgcgt gcggggtgcc gccgatcgac agcgtcaggg tcaccgcgag ccggtccgcg
     4021 aaccgatcgc cgccgccggc cccctgctgg gcgagatcga gcgcctgtgc ggtcatcgcc
     4081 ggccggcctc cgcgacctgc accgtgacat ggcgggtggt ggtgtcgatg tcgacgtcga
     4141 gcacgcacgc ctcgccgtcg acccggcccg cgatctcgaa gcgcaccagg ttggtgtgcc
     4201 agccaccgag cagccgcacc ctgggctcgc ggatccgcgg ctcgtgccgg ctcaccgcgg
     4261 cgagcagctc gacgcgcagc gcctcgacgg cgcggccggt gttgacctcg cgctcgtagt
     4321 cgccgagccc gaagtcggcg atcacgctgc cgcacgcctt gcgcgtgttg aacacgaagc
     4381 ccaggttgcg cgcgatctcc accggcgcgc gctcgggcaa gagctgcgcc agccgcccga
     4441 ggaacgcctg tcgccggcgc gtcggatcag ccatggtccg ggctctgcca gaacagcgcg
     4501 gcatgggtgt tctcgagccc gggcagcacg gcgaagcaca gcgcgcgctc gaccaccgcc
     4561 tggcgccact ccgggctggc ggcgtcgagc tggaacaccg tggcgcggtg gccgaaggtc
     4621 tgtgcgaagc ccagcgacgg gatcgcccgc cgcggcacgc cctcgagcgc cttggtgtag
     4681 acctcctcgg cgcgcagcgg gctcgccagc ttgagatcgt cgagcgccgg cggcgcggtg
     4741 cgctcggcgc ccggcgcctc ggacgctcgc tcgagcagga ggaacacctc gcgggcccag
     4801 cacagctcgt cggggaacgc cccggtgatg aaccagtgct cgtcgcgctg gaacgcaagc
     4861 cgcggcgaca cgacggcggg cacggacagc gtcgtggcga gcccgcgcgc cagccgctcg
     4921 aagcagccgc gcagatcgtc gtgctggtag cgcggcggcc agtcctcgag cggcttgccc
     4981 tgcagcacca ccgcctcgag gtagaagtcg cggagcgcgg cgaacaggtg gtaggggtgc
     5041 agcgccacgg cctgggtcgc ggcgccgtag ccgtgatcgg acagcacggc ctgcacgcgg
     5101 tagagcgcgg tgaggatccg gcggacctcg gacatcgact cggcgccggt cgcgctgtcc
     5161 gcggcgcgcc gcaccagctc gctctcgaac ccgccgagcg ccgccgcgat ctgggccatg
     5221 gtgtcgcgca ggaacggcgt ggtgccgctg cccagccgga tcagcggggg cacgtacggc
     5281 gccagcgccc atgcgccgtc ggagaagctc agctccgcca gcttcatccg ctcctgctcg
     5341 ctctcgatcc cgggctccgc cgagatctcg acctggtaga ccgcgcgccg gatctccggc
     5401 tcgtcggcct cgtagcgcgg cagatcgcgc gcgccgtcgg gccgcagctc gccgcgcagg
     5461 tggagataga ccggcaccgg cccggcggcc ttggcgggca ggacgagctt gcgcggggtc
     5521 aggaccgcgt tgcccgggac gtcgatcagg cggcccgagg gcagcaccac ggtgagcgcc
     5581 gagatcgaga gatcgccgct cgcgagctgg gcgtcgcccc agcggagcag cgccacgccg
     5641 cggctcggca gcccggtgag ctggacgcgc agcccgacgt gctcgagcag cgcctcctcc
     5701 tgggcgcgga actgggccgg gtgcagcacc tgccccatct cccagcgatg gagcgcgagc
     5761 cggtcgtagc tcggccgggg ctgccagtcc cgggcggcga ggacatcctg tgacgtgtgc
     5821 ggatccatgt gcccgtcctg acctgcggcc gccgacgagc tacttctcga ccttcatgcc
     5881 ccaggacttg accaccttgt cggagaacga ggtcgccacg gtgaccgtct gggccgacgg
     5941 ctgcggcttg atgccgatct ggaatgcgta gttctccggc gactggacct cgcccgaggc
     6001 gtcgtccgcg accgacaggt tcagatcggc gccgttcttc tcgagcaggc cgttgaggtc
     6061 ggagtcggtg gcggggagca tgcacttgaa gtacttcttc tcgatcgggt cgtacgagta
     6121 caccgagaac ttgaacacca cgtcgacctt gctcaggtcc ttgtagacca ggagctgggt
     6181 ctgctgcttg ttgtagaccg agatctgacc ggtgtaatac agcgtgtcgg tgacgccgag
     6241 ctcccacatg gcgcccgaca gcacggccac caccgggaag tcggtctccg gcgacatcgg
     6301 gtccttgacc gtctgatcgg ccttgagcgt gacgtcgccg atcttcagcg aggtcaagta
     6361 ccccaccggg gtctggatat ccttcttgaa attgtagcct tgcttgatgt cgcaggactt
     6421 ctggaacgtg ggcatggtcg aagctccttg ttcgggtcag ggcaggctag gcggcggccg
     6481 cgccgccgag ggcggcctcg agccggagct cgacgtccat gccctggaac ttgatgtgcg
     6541 gcagcaccga gaccgtgcag ctgtaccagc cgaacggccc cggcttcggc accaccgaga
     6601 ccgacatcgc cttgaacggg tagtacaaga gcgtcagatc atcgggattg gccgcggtgg
     6661 tgacgtactc gatcagccag gcctcgagca tcgactggat gtacggcgcg tcggcggtcg
     6721 agccgatgta gtcgcgtacc atgcgcttga cgtagtgcgc gatgcgcgtg atcgacagcg
     6781 tgtagtcgag gtagcacacc agatcggcgt tcttggtcgc cagatcctcc tcgaactcga
     6841 tcggcttctt gaccgcgcgc gcgctgaaga acgtcgcgtc cgccgtgccc ttgcagtgga
     6901 tcagggccat gaagccggtg ttggcgaact gcagctcgcg gtagtccggg atcgcgacct
     6961 ccaccggcgg ctgcagctcg aggtggccgt ggttcatcac catgtccacg ggcaggccgc
     7021 ggatcaggcc gccaccgcgc ggcccgacga tgtgctggca ccaccccgag tcctcgaacg
     7081 agcgcaccat gttgcgggcg aacagcaccg cggcgttgcc ccacaggtaa tcggtctcgg
     7141 ggttggggat cttctccacg aaggtcacga acttgctctt gccggtgttc tggtcgtacg
     7201 gcgcgcgcag catgtagcgc ggcagggtca ggccgatgta ggcggcgtgg tcggtggacc
     7261 ggaatgcatc ccagcggccg aactgcggca tgctgagcag ggtctcgaga tcgtccttgc
     7321 gctcgagctc cgcccagctg tcgaccccga agaactgcgg ccgggccgcg gcgacgaacg
     7381 ggcagtgcgc ggccgccgag atgtgcgaca tggtctccag ccactcgatg tcttcctcgg
     7441 tgctgtcgaa gtcgtacagg ccgatcatca cgccgaacgg gcggccgccg tagcggtcgt
     7501 actcctcgac atagaccttg cggaacagcg cgctgttgag gatataggcc gagtggtcct
     7561 ggagatcctg gccgagcgcg gccttgtcga tgtcgaggaa gtcgacgatg atctcgttcg
     7621 agttgacctc cgagcagatg tcctgcagcg agcgccagct cgcctcgagg cgctggaagc
     7681 tcgggctgtg caggacctgc tggatctgat cctggatcgc cgtgtcgagc gacacggaca
     7741 gccggtcgat ctccatcatc acccgccgga tgtcggtgac ctgggccaga tcgatgttga
     7801 ccaggaaggc gcccagcgag ttgatgaagc gatcctcgta cgggaagtcc tcgtcctgat
     7861 cgcggaactg caggttgacg atcgccggcg ccgcgggggt cgcgcctgca gcggcggggg
     7921 gcgcgcccgg cgcagtggtc gcgccag
//
LOCUS       DASVOR010002034         7142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_138444,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002034 DASVOR010000000
VERSION     DASVOR010002034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138444"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1446)
                     /locus_tag="VF516_37740"
     CDS             complement(<1..1446)
                     /locus_tag="VF516_37740"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8113553.1"
                     /translation="MPSDPTQPAARAAMSTACVLCSHNCGLVVAVEDGHLAQIGPDHR
                     NPITRGYICNKAYSLDRYVHHAQRVHHPLRRTAAGGFERVTWDRAVAEIADKLALLRR
                     KYTSRCIGLVGGGGQGNHMGVAYALSFLHGLGSRMWFNALAQEKTQHSLLDQWMFSAS
                     PATWLHADDHTTHYLLVLGSNPRVSHRGHNATEVLKAIAEDPDRTLVVVDPRRSETAR
                     QADLHVALRPGSDCYFLIGLIATLVRRNLIDAEFVRRYTEGADRLFAQIAPVDVAALA
                     AQCGIPGATIDRVAEGFARAPSAAIACDLGVEQTRFSTLNAYLIRVVLALTGNLGRAG
                     GNLFHETFNPPDPSLLEPRSSTFAHVSRTPGIRALSEFEMFSPSLVPEEVLGSHPARL
                     RALIVDGSNPLISYAATERWRAALRRLDLLVVIDPAMTETAAVADYVLPTPVGYEKWE
                     TSLFPKHARTIHAQLRPPLIARPSDALPEPEI"
     gene            complement(1461..2573)
                     /locus_tag="VF516_37745"
     CDS             complement(1461..2573)
                     /locus_tag="VF516_37745"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEX8113554.1"
                     /translation="MSPKVIAGPHASEASRATAAAVRNDPQYRELMNRLRAVGFFDAS
                     PWSFAWRIALCAGAYLGAFGYMLVAPSLWQRIAACCVIGIAHLRGNFIAHDASHGAIT
                     KNKLVVNLIGQFFDTFLGGYSFVYHRRVHDLHHYHCNEVDRDPNAMARLFALHDRAYG
                     DKNLFTRLTARVQHFIMLFGLPLWSFALRGESVAYVRRNWRKTRLDAVLLVAHVALWT
                     VLPVHYLGWWNAALCYVVMTAVTGVYLGTIVPVNHLAMPALEFDTAPSFLAQQLGTTR
                     NITSSPIHDLLFIGQNSQIEHHLFPWAPTFKLGRGRAIVREFCREHGIRYHECSFGTA
                     LREVRDHVVRLAQQVTATDPAEPAAASQPDLMQRAS"
     gene            complement(2846..4264)
                     /locus_tag="VF516_37750"
     CDS             complement(2846..4264)
                     /locus_tag="VF516_37750"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8113555.1"
                     /translation="MSTVLTQTLSLPFTSSGDAELPRVAQLFCVVDSGSPLRPASRHA
                     LDNVDEVKLVRSTRRGHQRGHTARGRQLILGIPDRWLSAEHACISRVDGDYVFRDLGS
                     KNGSQINGALVPSARLQDSDVIQIGQTCLIFRAGIPLSDTEPRDFMISAEQTIAGMNT
                     VNPVLSGRFSDLGRIARTDISVAILGETGTGKELVAHAIHDMSQRTGNFVPINCGALP
                     RDLAPSELFGYLKGAFTGATADKLGLIASAHQGTLFLDEIGDLPLEQQVVLLRVLQDR
                     SVTPVGGVTPRKVDFRLVVATHRDIPAALAAGTFREDLWTRASGFVLRLPPLRGRRED
                     LGLILGALLARQGASASNIALRGDATYALLQHGWPGNIRELEQCIKSALTLCDDGTVT
                     LAALQKSLRAESAARPAPDPASDHDPAEALDEQRQDHLRRLLERTSGNLSEVARLMGK
                     GRTQIQRWIKRYGIDADRYRHT"
     gene            complement(4320..6095)
                     /locus_tag="VF516_37755"
     CDS             complement(4320..6095)
                     /locus_tag="VF516_37755"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX8113556.1"
                     /translation="MELASRSEEIARLGNEIDVIVIGSGYGAAVCAARLAQAGAHVVV
                     LERGREFGRAVQRPFPSTPGQLRDNVQIDCELFSRKHRLGLYNFHLNRDIDVLVGCGL
                     GGTSLFNAGVVIKPDWRVFRRAAWPTKIVEAASPTSTSLDACFERARSVLDPAPYPPS
                     NIRPTPRKLSTMLAQGGERACLSIHYGDEPQNSVGVAQGKCIDCGDCITGCNFEAKKT
                     LCYTYLPIARSYGAKIFVQCDVSHIERVGDERWAVHFQRLTSGDEDVATDGQLEKLTA
                     RAVVVGAGVMGTAGILLRSKQRKLAISDKLGRRFSGNGDSVGVAYNCDQRLDSVGYGN
                     LVSGPPPWAPPAGADPVGPTILGVIDRRGTPNLDDGIIIQEGTFPSGMASLMRVLIQA
                     VAGVTGSETQHGFAHWFHERIAEGRDLFGNTRDGALNRSMTFLVMGHDGADGVIELAC
                     DGRPVVRWPELHTRGVFTTEDNLARTITGRLGGMYVKDPLNTPLLMNNHLTVHPLGGC
                     PMGDSGAGGVVDFAGRVFSDDGRTYRGLYVSDASVIPTSLGVNPLWTITALAEWIAGH
                     VATDLGLTPDPFPGRAPEDHRPDAW"
     gene            complement(6209..6793)
                     /locus_tag="VF516_37760"
     CDS             complement(6209..6793)
                     /locus_tag="VF516_37760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113557.1"
                     /translation="MAGNGVDIVRTGTSVHPAVEPAIDMAEQVPAEETLHVDPVQVDP
                     APVNAAPVNPTLVYAFLGFLVALDVVLATCALAFPQTWFAIMHGSVVYDDPAGLLYRT
                     GAVWVAFTLLQAIALVRWRRAPYWLALVAGVRLTELFSDWATLAAAQHVTRFAWLALL
                     TAPLSNLLFGWFLIATYRRLDPTPPHPGFTPPAR"
BASE COUNT         1082 a         2476 c         2546 g         1038 t
ORIGIN      
        1 gatctccggc tcggggagcg cgtcggacgg acgcgcgatc agcggcggcc gcagctgcgc
       61 gtggatcgtc ctggcgtgct tggggaacag cgaggtctcc cacttctcgt agccgaccgg
      121 cgtcggcagg acatagtccg cgaccgccgc ggtctcggtc atcgccgggt cgatcacgac
      181 cagcaggtcg agccggcgca gcgcggcgcg ccagcgctcg gtcgcggcgt acgagatcag
      241 cgggttggag ccgtcgacga tcaacgcccg cagccgcgcc ggatgactgc cgagcacctc
      301 ctcgggaacg agcgacggcg agaacatctc gaactcgctg agcgcgcgaa tgcccggcgt
      361 ccgcgacacg tgggcgaagg tcgacgagcg cggctcgagc agcgacggat ccggcggatt
      421 gaacgtctcg tggaacaggt tgccgccggc gcgcccgagg tttccggtca gcgcgagcac
      481 gacccggatc aggtacgcgt tcagcgtgga gaaccgggtc tgttcgacgc cgagatcgca
      541 cgcgatcgcc gccgacggcg cgcgggcgaa gccctcggcg acgcgatcga tcgtcgcgcc
      601 cggaatcccg cactgcgccg ccagggcggc gacgtcgacc ggcgcgatct gcgcgaacag
      661 ccggtcggcg ccctcggtgt agcggcgcac gaactccgcg tcgatcaggt tgcgccgcac
      721 cagcgtcgcg atcaggccga tcaggaagta gcagtcggac ccggggcgca gcgcgacgtg
      781 gaggtcggct tgacgggcgg tctcggagcg gcgcggatcg accacgacca gcgtgcgatc
      841 gggatcctcg gcgatcgcct tgagcacctc ggtggcgttg tggccgcggt gactgacccg
      901 cgggttagag ccgagcacga gcaggtagtg cgtcgtgtga tcgtcggcgt gtagccaggt
      961 cgccggggac gcgctgaaca tccattgatc gagcaggctg tgctgcgtct tctcctgcgc
     1021 cagcgcgttg aaccacatcc gcgatcccag cccgtgcagg aagctcagcg cgtaggcgac
     1081 gcccatgtgg ttgccctggc cgccgccgcc gaccagcccg atgcagcggc tggtgtactt
     1141 ccggcgcagc agggccagct tgtcggcgat ctccgcgacc gcccggtccc aggtcacccg
     1201 ctcgaacccg ccggccgcgg tccgccgcag cgggtggtgg acgcgctgcg cgtgatggac
     1261 gtagcggtcc agcgagtagg ccttgttgca gatgtagccg cgggtgatcg ggttgcgatg
     1321 gtcgggcccg atctgcgcga gatggccgtc ctcgaccgcg accacgaggc cgcagttgtg
     1381 gctgcacagc acgcacgccg tggacatcgc ggcgcgcgcg gcgggctggg tcgggtcgga
     1441 cggcatcgct cgctcgctcc ctatgatgcc cgctgcatca ggtcgggttg cgacgcggcg
     1501 gcgggctccg cggggtcggt cgccgtcacc tgctgcgcca gccggaccac gtgatcgcgg
     1561 acctcgcgca gcgcggtgcc gaacgagcac tcgtggtacc ggatgccgtg ctcgcggcag
     1621 aactcgcgaa cgatcgcccg gccgcgcccg agcttgaacg tcggcgccca cgggaacagg
     1681 tggtgctcga tctgcgagtt ctgcccgatg aacagcagat catggatcgg ggacgacgtg
     1741 atgttgcggg tcgtgccgag ctgctgcgcg aggaacgacg gcgcggtgtc gaactcgagc
     1801 gccggcatcg cgaggtggtt gaccgggacg atcgtgccga ggtagacgcc ggtgacggcg
     1861 gtcatcacca cgtagcacag cgcggcgttc caccagccca ggtaatggac cggcagcacc
     1921 gtccacagcg cgacgtgcgc gaccagcagc accgcatcga gccgggtctt gcgccagttg
     1981 cggcggacat aggccacgct ctcgccgcgc agcgcgaagc tccacagcgg aagcccgaac
     2041 agcatgatga agtgctggac ccgcgcggtg agccgggtga acaggttctt gtcgccgtac
     2101 gcccggtcgt gcagcgcgaa cagccgcgcc atcgcgttcg gatcgcggtc gacctcgttg
     2161 cagtggtagt ggtgcaggtc gtggacgcgc cggtggtaga cgaacgagta cccgccgagg
     2221 aacgtgtcga agaactggcc gatgaggttc acgaccagct tgttcttggt gatcgcgccg
     2281 tggctggcgt cgtgcgcgat gaagttgccg cgcaggtgtg cgatcccgat gacacagcac
     2341 gcggcgatgc gctgccacag cgatggcgcc accagcatgt acccgaacgc gccgaggtag
     2401 gcccccgcgc acagcgcgat gcgccaggcg aagctccacg ggctcgcgtc gaagaacccg
     2461 accgcgcgca gccggttcat gagctcgcgg tactgcggat cgttgcgcac cgccgccgcc
     2521 gtggcgcgcg atgcctccga ggcgtggggg cccgcgatca ccttcggaga cacgtagtca
     2581 tccaggatga agttcgcaaa gctcttcccg gaccgttctc ggtctgggag atgaagattg
     2641 ggctgcattc gtctgacctt tccggggggg aggggcgggg gcgatcccga caaggcaagg
     2701 accgtgccgc gcgtcgcgtg tggtcctcaa gccccgacgg cgggatcgta gagtcaccgg
     2761 cacggcacga cccggcacgc tctgccacgg atggcgccgg cggccgctac cggtcgtggc
     2821 ggggggtgcc acacatcgtg gcgcctcagg tgtggcggta gcgatccgcg tcgatgccgt
     2881 agcgcttgat ccagcgctgg atctgcgtcc ggcccttccc catcaaccgc gcaacttcac
     2941 tcagatttcc tgacgtgcgc tccagcagcc tgcgcaggtg gtcctggcgc tgctcgtcga
     3001 gcgcctccgc gggatcgtgg tcggaggcag gatccggcgc cggccgggcc gcggactcgg
     3061 cgcgcagcga tttctgcagc gccgcgaggg tcacggtgcc gtcatcgcac agggtcagcg
     3121 cggacttgat gcactgctcg agctcgcgga tgtttcccgg ccagccgtgc tggagcagcg
     3181 cgtaggtcgc gtcgccgcgc agcgcgatgt tcgacgcgct cgcgccctgg cgcgccagca
     3241 gcgcgccgag gatgagcccg agatcctcgc gccgtccgcg cagcgggggc agccgcaaca
     3301 cgaagcccga cgcccgcgtc cacaggtcct cgcgaaacgt ccccgcggcg agcgccgccg
     3361 ggatatcgcg gtgggtcgcg acgaccaggc gaaagtcgac cttgcgaggc gtcacgcccc
     3421 cgaccggcgt caccgaacgg tcctggagca cgcgcaacag cacgacctgc tgctcgagcg
     3481 gcaggtcccc gatctcgtcg aggaacagcg tgccctggtg cgccgacgcg atcaggccga
     3541 gcttgtccgc ggtggcgccg gtgaaggccc ccttgaggta tccgaacagc tcgctcggcg
     3601 ccaggtccct gggcagggcg ccgcagttga tcggcacgaa gttgccggtg cgctgcgaca
     3661 tgtcgtggat cgcgtgcgcg accagctcct tgccggtccc ggtctctccg aggatcgcca
     3721 ccgagatgtc ggtgcgcgcg atccggccga gatcgctgaa tcgcccgctg agcaccgggt
     3781 tcacggtgtt cattccggca atggtctgtt ccgcggagat catgaaatcg cgcggctctg
     3841 tatccgacag cggaataccg gcgcggaaga tcaggcaggt ctgtccgatc tggatgacgt
     3901 ccgagtcctg cagccgcgcg gacgggacca gggcgccgtt gatctgcgac ccgttcttgg
     3961 agccgaggtc ccggaacacg tagtcgccgt cgacccgcga gatgcaggcg tgctccgcgg
     4021 acagccagcg atcggggatc ccgaggatca gctgcctgcc gcgggcggtg tggccgcgct
     4081 ggtggccgcg ccgcgtcgag cgcaccagct tgacctcgtc gacgttgtcg agcgcgtggc
     4141 gcgacgccgg gcgcagcgga gagccgctgt cgaccacgca gaacagctgc gcgacgcgcg
     4201 gcagctcggc gtcaccgctc gaggtgaacg ggagcgacag cgtctgggtc agcaccgtgc
     4261 tcatccgcgc ccgccgcagc ttaccgcgac cgcccggccg gtaccaccgc ggccggccgt
     4321 caccaggcgt ccggtcgatg gtcctcggga gcacgcccgg ggaacgggtc gggcgtcaat
     4381 ccgaggtcgg tcgcgacatg gcccgcgatc cactcggcga gcgcggtgat cgtccacagc
     4441 gggttcacgc cgagcgacgt cgggatcacc gacgcgtccg agacgtagag cccgcggtag
     4501 gtccggccat cgtcgctgaa cacccgcccc gcaaagtcga ccacaccgcc ggcgccgctg
     4561 tctcccatcg ggcagccgcc gagggggtgc accgtgaggt ggttgttcat gagcagcggc
     4621 gtgttgagcg ggtctttcac gtacatgccg ccgagccggc cggtgatcgt gcgcgcgagg
     4681 ttgtcctcgg tggtgaacac gccccgcgtg tggagctcgg gccagcgcac gaccggccgg
     4741 ccgtcgcagg cgagctcgat gacgccatcc gccccgtcgt ggcccatcac caggaacgtc
     4801 atcgagcggt tgagggcgcc gtcgcgggtg ttgccgaaca ggtcgcgacc ctcggcgatg
     4861 cgctcgtgga accagtgcgc aaacccgtgc tgggtctcgg acccggtcac gccggcgacc
     4921 gcctggatga gcacgcgcat caggctggcc atgccgctcg ggaacgtgcc ttcctggatg
     4981 atgatgccgt cgtcgaggtt cggcgtgccg cggcgatcga tgaccccgag gatcgtcgga
     5041 ccgaccgggt cggccccggc cggaggcgcc cacggcggtg ggccggacac caggttgccg
     5101 tagccgaccg agtcgagccg ctgatcgcag ttgtaggcga ccccgaccga gtcgccgttc
     5161 ccgctgaacc ggcggccgag cttgtcggag atcgccagct tgcgctgctt cgagcgcagc
     5221 aggatccccg ccgtccccat caccccggcg cccacgacca ccgcgcgcgc cgtgagcttc
     5281 tcgagctggc cgtccgtcgc gacgtcctcg tcgccgctgg tcaggcgctg gaagtggacg
     5341 gcccagcgct cgtcgccgac gcgctcgatg tggctgacgt cgcactgcac gaagatcttg
     5401 gcgccgtacg acctcgcgat cggcaggtac gtgtaacaca gcgtcttctt cgcttcgaag
     5461 ttgcagccgg tgatgcagtc gccgcagtcg atgcacttgc cctgtgccac cccgaccgag
     5521 ttctggggtt cgtctccgta gtggatgctc aggcaggcgc gctcgccgcc ctgcgccagc
     5581 atcgtcgaga gcttgcgcgg cgtcggccgg atgttcgacg gggggtacgg cgcgggatcc
     5641 agcacgctgc gcgcgcgctc gaagcacgcg tcgagcgacg tgctcgtggg cgatgccgcc
     5701 tcgacgatct tcgtcggcca cgcggcgcgc cggaacaccc gccagtccgg cttgatcacc
     5761 acgccggcgt tgaacagcga ggtgccgccg aggccgcagc cgaccagcac atcgatgtcc
     5821 cggttgagat ggaaattgta gagtccgagc cggtgcttgc gcgagaacag ctcgcagtcg
     5881 atctgcacgt tgtcgcggag ctgcccgggt gtgctcggga acggtcgctg gaccgcgcgc
     5941 ccgaactcgc gaccgcgctc cagcacgacg acatgcgccc cggcctgcgc cagccgggcc
     6001 gcacacaccg ccgcgccgta ccccgaaccg atcacgatca catcgatctc gttccccagc
     6061 cgggcaatct cctcactgcg gcttgccagc tccatggcgt ctcccccgtc tatggttgtg
     6121 agcagtcgat gcggcggtcc cctgcaacca gcgggcccgg ccctggccgc cccaggaccg
     6181 gtccggtcgc tggacgcgcg atcgtacgtt atcgagccgg gggagtgaac ccgggatggg
     6241 gcggggtcgg atccagccgc cggtacgtcg cgatcaggaa ccacccgaac agcaggttcg
     6301 acagcggcgc ggtcaggagc gccagccagg cgaaccgcgt cacgtgctgg gccgccgcca
     6361 gcgtcgccca gtcggagaac agctcggtga gccggacgcc ggcgaccagc gcgagccagt
     6421 agggcgcgcg ccgccagcgc accagcgcga tcgcctggag cagcgtgaaa gcgacccaca
     6481 cggcgccggt gcggtacagc agacccgccg ggtcgtcgta caccaccgac ccgtgcatga
     6541 tcgcgaacca ggtctgcggg aacgccagcg cgcaggtcgc cagcacgacg tcgagcgcga
     6601 ccaggaagcc caggaacgcg tacaccagcg tcgggttgac gggcgccgcg ttgaccggcg
     6661 ccgggtcgac ctggaccggg tcgacgtgca acgtctcctc cgccggaacc tgctccgcca
     6721 tgtcgatcgc cggctcgacg gccggatgga cggatgtccc ggttcgaacg atgtccacac
     6781 cattgcctgc catcacaccc tccttgcatc gcgatcagac catatcgcgc gcgatcgtca
     6841 agcgatcgag gcgcggacct gacgccgcca cggccagcgc gcaagcgtcg tacagcggtc
     6901 gatcccacgg ggggctctgc ccccgcctcg cctcgctcga cagcgccgcc gcccggtttc
     6961 gtcgagcggg gtgttcaggt gtaatacccg ggatgcgagg aaccaccgcc gggttcggct
     7021 cgatcgaggt cctcgtccgg gccttgctcg acgagttccc cgagctcgac ccccggcggg
     7081 tgcgcgcggc ggtcgagcgc gccaccgcca aggtcgcgca cgccgcgctc gataccgagg
     7141 gc
//
LOCUS       DASVOR010002035         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_372309,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002035 DASVOR010000000
VERSION     DASVOR010002035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372309"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..526
                     /locus_tag="VF516_37765"
     CDS             149..526
                     /locus_tag="VF516_37765"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEX8113558.1"
                     /translation="MEPSEVVRALWHRFQDHNWTGAGELIAEDAVFDWPASRERLVGR
                     DKIIAVNREYPAGWSIKVLRVVAEDDQVAAEVEVPHTELGLFRAAGFYAVRDGQIIHG
                     TEYWTTVGADEPGGDRAHLVERY"
     gene            complement(536..1891)
                     /locus_tag="VF516_37770"
     CDS             complement(536..1891)
                     /locus_tag="VF516_37770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8113559.1"
                     /translation="MRKKDFTTLVAGLFGVDQRHGGPCGMRQEGGTGRTDQLRAYRPT
                     GGMLRSRHRTHSVRRARTRIGDGGWPVMQRDIFTSDHDMFRDAVRSFIAKEVAPYHEQ
                     WERDGVVSRDVWLAAGEAGLLGIDMEERFGGGGNDDYRYYVVLNEEMNRAGAHGPAFS
                     VHNDINGQYFKRLMTDEQAQRWMPGYCSGELISAIAMSEPGAGSDLQGIRTTAVRDGD
                     SYVVNGQKTFISNGQLADLVIVVARTSPDAGHRGISLLVIERGMPGFERGRNLDKVGM
                     HAQDTSELFFSDVRVPSANLLGEEGGGFIALMQNLPRERVAIGATALAGAEAVFEQTL
                     AYCKQRQAFGRPIGKFQHNRFVLAEMATELAVARAFTDKAVLEHNAGRLSNEDASMVK
                     WWDTELCNRVVDRCLQLHGGYGYMREYAVAKAFADGRVQTIFGGTTEIMKEIIGRGLG
                     V"
     gene            1907..>2152
                     /locus_tag="VF516_37775"
     CDS             1907..>2152
                     /locus_tag="VF516_37775"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8113560.1"
                     /translation="MSFTSQDDEPATLSAAQPGTPENPLEISDPRALRALAHPGRLAI
                     LQHLVLDGPATATECAEVAGLSPSACSYHLRALARYGF"
BASE COUNT          348 a          754 c          672 g          378 t
ORIGIN      
        1 gcctccgcca gcgatcacca ccgcatggtc ggccattcga cactccttcg cttccggcac
       61 ctgtcgctca gtctgagact gtgcaccacc agagtcctgc agcactcggc ggggctggga
      121 tgtcggtggt ggctgggagg atggccccat ggagccatcc gaggttgtgc gcgcgctctg
      181 gcaccggttt caggaccaca actggaccgg cgcgggcgag ctcatcgcag aagacgcggt
      241 cttcgactgg cccgccagcc gggagcgcct ggtcggcagg gacaagatca tcgcggtaaa
      301 cagggagtac ccggcgggct ggtcgatcaa ggtactccgg gtcgtggcgg aagacgacca
      361 ggtcgcagcc gaggtcgagg tgccgcatac ggagctcggc ctgttccgtg ctgcagggtt
      421 ctacgcagtg cgggacggcc agatcatcca tggcaccgag tactggacga ccgtcggcgc
      481 ggacgagccc ggcggcgatc gggcacatct cgtcgagcgg tactgacagc gatcctcaga
      541 cgcctagccc gcggccgatg atctccttca tgatctccgt cgtgccgccg aagatcgtct
      601 gaaccctgcc gtcggcgaac gccttggcga ccgcgtactc gcgcatgtag ccgtagccgc
      661 cgtgcagctg caggcagcgg tcgaccacgc gattgcacag ctcggtgtcc caccacttca
      721 ccatcgaggc atcttcgtta gagagccggc cagcattgtg ctcaagcact gccttgtcgg
      781 tgaacgcgcg ggccacggca agctcggtcg ccatttcggc caggacgaac ctgttgtgct
      841 ggaacttgcc gatcggccgg ccgaaagcct gccgctgctt gcaatacgcc agcgtttgtt
      901 cgaatacggc ctccgccccg gcgagcgccg tggcgccgat ggcgacccgc tccctcggca
      961 ggttctgcat cagcgcgatg aacccgccgc cctcttcgcc gagcaggttc gccgagggca
     1021 cccggacgtc gctgaagaac agctcggagg tgtcctgtgc gtgcataccg accttgtcca
     1081 ggttgcggcc gcgctcgaaa ccaggcatcc cccgctcgat caccagcagg ctgatcccgc
     1141 ggtgaccggc gtcgggactc gttcgcgcga ccacgatgac caggtcggcg agctggccgt
     1201 tggagatgaa cgtcttctgc ccgttgacca cgtagctgtc accgtcgcga accgcggtcg
     1261 tcctgatccc ctgcaggtcg ctgccggcgc ccggctcgct catcgcgatc gcggagatga
     1321 gctcaccgct gcagtacccg ggcatccagc gctgcgcctg ctcatcggtc atcagccgct
     1381 tgaagtactg gccgttgatg tcgttgtgca ccgagaacgc gggcccatgg gctccggccc
     1441 ggttcatctc ctcgttcagc accacgtagt agcggtaatc gtcattgccg ccgccgccga
     1501 agcgctcctc catgtcgatc ccgagcagtc ccgcctcgcc cgccgccagc cacacatcgc
     1561 gcgaaacgac gccgtcccgc tcccactgtt cgtgatacgg cgcgacctcc ttggcgatga
     1621 acgagcgcac cgcgtccctg aacatgtcgt ggtcgctcgt gaagatgtcg cgctgcataa
     1681 ctggccatcc cccgtcgccg atccgcgtcc gcgcccgcct caccgagtga gtccggtgac
     1741 gtgaacgtag catcccgccg gtcgggcgat aagccctgag ttgatccgtt cgtcccgtac
     1801 cgccctcctg tcgcatcccg cacggcccac catgacgctg atcaacgcca aagaggccgg
     1861 cgacaagagt tgtgaagtct ttctttcgca actaagcttt cgcaacatgt ccttcacatc
     1921 gcaggacgac gagcctgcga ctctgagcgc agcgcagccg ggtaccccgg agaaccccct
     1981 ggagatttcc gatccgcggg cgctgcgtgc gctcgctcat cccggcaggc tagcgatcct
     2041 gcagcatctc gtgctggatg gaccggcgac ggctaccgag tgcgccgagg tggcgggcct
     2101 ctcaccgtcg gcatgcagct accatctgcg cgcgctcgcg cggtacgggt tc
//
LOCUS       DASVOR010002036         3664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_372314,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002036 DASVOR010000000
VERSION     DASVOR010002036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372314"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VF516_37780"
     CDS             <1..534
                     /locus_tag="VF516_37780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113561.1"
                     /translation="PGASGEASGEASSGASSEASDEATRVKGSARLIAFRQVGEQVEQ
                     LEPDAVVRAGDVIQLRYNAAGRSYGVIASVDGAGVVTLHYPLREDAPPEATAMAPEPT
                     ALPHAYALDSAPRFERFFFVTAKDPLDVRQTLAALRVLAHREDSATARLELPAGLRQW
                     SLRLRKADRTSSNHESP"
     gene            543..2333
                     /locus_tag="VF516_37785"
     CDS             543..2333
                     /locus_tag="VF516_37785"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8113562.1"
                     /translation="MTSRTVLCLAFAALLALPTSAPVHASSAAAPLRRFALVIGSNNG
                     GNVRERLRYAGHDAETIADVLRQLGGVDQVDLDLLSEPDARGLDHAFDVLSDRVRAER
                     KRGQRVELVVYYSGHADETGILLGGAHYEYARLRQRIRDVPADVHIAIVDSCASGSFT
                     RIKGGARKPPFLHDSSNQVQGFAFLSSSSADEDAQESDKIGASFFTYFFVTGLRGAAD
                     RNHDGKITLTEAYQFAYEQTLGRTQNTTHGPQHPAYDMQLSGTGDVVITDLRSTEATL
                     VLPSALRGRVTVVDPSGRVAVELTKEAGEPLSLALPDATYAVHVDSGNGEFVATVTLA
                     RGGELAFDPGALHRTSPERTVARGPARGDEDGEREEDGDDDDHDDGNRDGERHHRGRH
                     SPWYRRIAASFGEGLRVERPQAIPGLMNHDPSQPFMTQKPDFDALITLGGTSREVHGS
                     PVIGVAETGDLSLGTTLGHTIGFAYDMSFGFGPGVFLGDNLQVGATIGFGLSGITGGV
                     LPFAWKVPTEAFVILELSPEVRPMAYFRQDYLFGADARKHGSTLARWGDEAEAGAGLR
                     FSGKLDGFVYGSVREMAGVRYWGIGMGAVL"
     gene            2354..3364
                     /locus_tag="VF516_37790"
     CDS             2354..3364
                     /locus_tag="VF516_37790"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8113563.1"
                     /translation="MQSLSSWLRGARIRAGMIAAVAALVGCGANAGASLTPGMAKLPA
                     ADATPLAVHRWPVAPARAKAVVLIVHGASEHAARYDRFARFLNENGYAVYAMDLRGHG
                     QTRLRSGALLDAGPDAWNHFVDDQKWLRDLIGRELPGKKVVLLGHSMGSAIAQDYLTR
                     YGKTVDAYILSGTFYGPALPDDLLKTLDDAAAKAPLEPSEIFAGVFANFNKPFSDKPG
                     FDWLSRDPAEVAKYVNDPLCGKPFGNELTRDFFRGLSKMRAPEVEAKIPKDVPIHIVV
                     GDQDPVGENTRTVMALIDRYHALGLTKVSSKFYPQARHELLNETNRDEVQKDLLDWLN
                     AL"
BASE COUNT          537 a         1217 c         1411 g          499 t
ORIGIN      
        1 ccgggggcgt ccggcgaggc gtccggcgag gcgtccagcg gggcgtccag cgaggcgtcc
       61 gatgaagcga cgcgggtgaa ggggtcggcg cggctgatcg cgttccggca ggtcggcgag
      121 caggtggagc agctggagcc ggacgcggtg gtgcgcgccg gggatgtgat ccagctgcgc
      181 tacaacgcgg ccggccggag ctacggggtg atcgcgtcgg tcgacggggc cggggtggtg
      241 acgctgcact atccgctgcg cgaggacgcg ccgcccgagg cgacggcgat ggcgccggag
      301 ccgaccgcgc tgccgcacgc gtatgcgctc gacagcgcgc ctcggttcga gcggttcttc
      361 ttcgtcaccg cgaaggaccc gctggacgtc cggcagacgc tcgcggcgct gcgcgtgctg
      421 gcccatcgcg aggacagcgc gacggcgcgg ctggagctgc cggccgggct gcgccagtgg
      481 tcgctgcgcc tgcgcaaggc ggatcgcact tcttccaacc acgagtcgcc atgaggatcg
      541 ccatgacttc ccgcaccgtg ctttgcctcg ccttcgccgc gctgctcgcc ctgccgacga
      601 gcgcgccggt tcacgccagc tcggcggccg cgccgctgcg gcggttcgcg ctggtgatcg
      661 gcagcaacaa cggcggcaac gtgcgcgagc ggctgcgcta cgccgggcac gacgccgaga
      721 cgatcgccga tgtcctgcgc cagctcggcg gggtcgacca ggtcgatctc gacctgctga
      781 gcgagccgga cgcgcgcggg ctcgaccacg cgttcgacgt gctgtcggat cgcgtccgcg
      841 ccgagcgcaa gcgcgggcag cgggtcgagc tggtggtgta ctactcgggg cacgccgacg
      901 agaccgggat cctgctgggc ggggcgcatt acgagtatgc gcggctgcgc cagcggatcc
      961 gcgacgtgcc ggcggacgtg cacatcgcga tcgtcgacag ctgcgcgtcg gggagcttca
     1021 cgcggatcaa gggcggcgcg cggaagccgc cgttcctgca cgacagctcg aaccaggtcc
     1081 aggggttcgc gttcctgtcg tcgagctcgg ccgacgagga cgcgcaggag tcggacaaga
     1141 tcggcgcgtc gttcttcacg tacttcttcg tgaccgggct gcgcggcgcg gcggaccgca
     1201 accacgacgg caagatcacg ctgaccgagg cgtaccagtt cgcgtacgag cagacgctgg
     1261 ggcgaacgca gaacacgacg cacgggccgc agcacccggc gtacgacatg cagctgtcgg
     1321 ggaccgggga cgtggtgatc acggacctgc gcagcacgga ggcgacgctg gtgctgccgt
     1381 cggccctgcg cggacgggtc acggtggtcg acccgagcgg gcgggtcgcg gtcgagctga
     1441 ccaaggaggc gggcgagccg ctcagcctgg cgctgcccga cgcgacctac gcggtgcacg
     1501 tcgacagcgg gaacggtgag ttcgtggcga ccgtgacgct ggcccgcggc ggcgagctgg
     1561 cgttcgaccc gggggcgctg catcgcacgt cgccggagcg cacggtggcg cgcgggcccg
     1621 cgcgcgggga cgaggacggc gagcgcgagg aggatggcga cgacgacgac cacgacgacg
     1681 gcaaccgcga cggcgagcgc caccaccgcg gccggcactc gccgtggtac cggcggatcg
     1741 cggcgagctt cggcgagggg ctgcgggtcg agcggccgca ggcgattccg ggcctgatga
     1801 atcacgaccc gagccagccc ttcatgacgc agaagccgga cttcgacgcg ctgatcacgc
     1861 tgggcgggac gtcgcgcgag gtccacggca gcccggtgat cggggtcgcc gagaccgggg
     1921 acctgtcgct cggcacgacg ctcggccaca cgatcggctt tgcctacgac atgagcttcg
     1981 ggttcgggcc gggggtgttc ctcggcgaca acctgcaggt gggcgcgacg atcgggttcg
     2041 ggctgagcgg gatcaccggc ggcgtgctgc cgttcgcgtg gaaggtgccg accgaggcgt
     2101 tcgtgatcct cgagctgtcg cccgaggtgc ggccgatggc gtacttccgg caggactacc
     2161 tgttcggcgc cgacgcgcgc aagcacggct cgacgctggc gcgctggggc gacgaggccg
     2221 aggccggcgc ggggctgcgg ttcagcggga agctcgacgg cttcgtctac ggcagcgtgc
     2281 gcgagatggc cggggtgcgc tactggggca tcgggatggg cgcggtgctg tagcggcgtg
     2341 atagcgtcgc cggatgcaaa gcttgtcctc ctggctgcga ggcgcgcgga tccgcgccgg
     2401 catgatcgct gcggtcgccg cgctggtcgg ctgcggcgcg aacgcggggg cgtcgctcac
     2461 gcccggcatg gcgaagctgc cggccgcgga cgcgaccccg ctcgcggtgc accgctggcc
     2521 ggtggcgccg gcccgcgcga aggccgtcgt gctgatcgtg cacggcgcgt cggagcacgc
     2581 ggcgcgctac gatcggttcg cgcggttcct gaacgagaac gggtatgcgg tgtacgcgat
     2641 ggacctgcgc ggccacggcc agacgcggct gcgctcgggg gcgctgctcg acgccgggcc
     2701 cgacgcctgg aaccacttcg tcgacgacca gaagtggctg cgcgacctga tcgggcgcga
     2761 gctgccgggc aagaaggtgg tgctgctcgg ccacagcatg gggtcggcga tcgcgcagga
     2821 ctacctgacg aggtacggca agaccgtcga cgcgtacatc ctgagcggca cgttctacgg
     2881 gccggcgctg cccgacgacc tgctgaagac gctcgacgac gcggccgcga aggcgccgct
     2941 cgagccgtcg gagatcttcg ccggcgtgtt tgccaatttc aacaagccgt tctccgacaa
     3001 gccgggattc gactggctga gccgcgatcc ggcggaggtc gcgaagtacg tgaacgaccc
     3061 gctgtgcggc aagccgttcg gcaacgagct gacgcgcgac ttcttccgcg ggctgagcaa
     3121 gatgcgggcg cccgaggtcg aggcgaagat ccccaaggac gtgccgatcc acatcgtcgt
     3181 gggcgatcag gatccggtcg gcgagaacac caggaccgtg atggcgctga tcgatcgcta
     3241 tcacgcgctc gggctgacca aggtgagctc caagttctat ccccaggcgc ggcacgagct
     3301 gctcaacgag accaaccgcg acgaggtgca gaaggatctg ctggactggc tcaacgcgtt
     3361 gtagcgtgtg cggcgattca caccgcgggc ccgcggcggc gcgatcgcga caggaatgtc
     3421 gggcaagaag tatgacctgg ctgtcgtgat ccagccgggg cggcgaaatc tggcaagcca
     3481 gtgttccgag gaaactcggg cggttgccgt ggcaccccac ttgccagaac atgtcggcat
     3541 gcgcccctcc ctctcgctca ccgtgctcgc cgcgctgctc gccggctgca gcggctcgga
     3601 ccgcgaccgc gcgcccggcc agacccagtt cgagtccgca cctcccggcg gtggcgccgg
     3661 cggg
//
LOCUS       DASVOR010002037         2647 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_489314,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002037 DASVOR010000000
VERSION     DASVOR010002037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2647
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489314"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VF516_37795"
     CDS             <1..462
                     /locus_tag="VF516_37795"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX8113564.1"
                     /translation="GLVKALRYMGLAPGEPLLGRPVDVVFIGSCTNARLTDMRAAASV
                     MRGNKVAAGVRVLVVPGSKDVKRQAEAEGLDRVFRDAGAEWRDPGCSMCIAMNGDRLE
                     PGQYAVSTSNRNFEGRQGPGGRTFLASPLTAAAAAVTGRITDVRTLTQEPR"
     gene            459..1034
                     /gene="leuD"
                     /locus_tag="VF516_37800"
     CDS             459..1034
                     /gene="leuD"
                     /locus_tag="VF516_37800"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF002458.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEX8113565.1"
                     /translation="MTPFTTLESTVVVLPLDDIDTDQIIPARFLQGTDKAGLGEHLFA
                     DCGRHPDFPLNEIGARFAKILVAGANFGCGSSREHAAWALVDRGFRAILARSFGDIFR
                     QNAIKNGLLPVALPADAHARVVAARAADPRAIVRVDLPAQRVAIAGDAAASFGFAIDP
                     FARHCLVHGVDELGYLLGFQDRITEFERRPR"
     gene            1031..2113
                     /gene="leuB"
                     /locus_tag="VF516_37805"
     CDS             1031..2113
                     /gene="leuB"
                     /locus_tag="VF516_37805"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEX8113566.1"
                     /translation="MNATIVLLPGDGIGPEVVREGQQVLAAIAARAGHQLAFESHPIG
                     GAALNQHGDPLPPATLEVCTRADAILLGAVGGPKWDDPRARQRPEQGLLGLRKQLGLY
                     ANLRPVRVHPALAAASPLQPALLAAVDVLFVRELTGGLYFGRPRLRETVDGRRHAIDT
                     LEYSDHEIARVVRLAFRLARGRNGRVTSVDKANVLESSRLWREVASEIAAEFPDVALE
                     HQLVDSCAMRIVSAPRSFDVVVTENMFGDILTDEAAALAGSLGLIPSASLGDGTRGLY
                     EPIHGSAPDLAGKDAAHPIGTILSAAMLLRYSLGLAREADLVERAVASAIAAGARTAD
                     MGAPQGTALSTTAMGERIRREILERG"
     gene            2167..>2647
                     /locus_tag="VF516_37810"
     CDS             2167..>2647
                     /locus_tag="VF516_37810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113567.1"
                     /translation="MRSWMALLALAPACGFSSSAGSGTGKIDAGDAGPACFGAFPRVC
                     FPASAVPMKPRTLDGLTEIDTDMTASGSACDQNNDQKAAYCVYASAGFTLAASAMLTA
                     HGSKPLVVLSTTMMDLAGSIDVSSHLAGAPQLRGAGANPQDPAACAFRTPPTAAAMGG
                     "
BASE COUNT          356 a          991 c          946 g          354 t
ORIGIN      
        1 ggtctcgtga aggcgctgcg ctacatgggc ctcgcgcccg gcgagccact gctcggccgc
       61 ccggtcgacg tcgtgttcat cggctcgtgt acgaacgcac ggctcaccga catgcgcgcc
      121 gcggcgagcg tgatgcgcgg caacaaggtc gcggccggcg tgcgcgtgct cgtcgtgccg
      181 ggcagcaagg acgtcaagcg ccaggccgag gccgaggggc tcgaccgggt gttccgcgac
      241 gccggcgccg agtggcgcga cccgggctgc tcgatgtgca tcgcgatgaa cggcgaccgg
      301 ctcgagcccg gccagtacgc cgtcagcacc agcaaccgca acttcgaggg ccggcagggc
      361 ccgggcggcc ggacgttcct cgcgtccccg ctcaccgccg ccgcggccgc cgtcaccggc
      421 cggatcaccg acgtcaggac cctgacccag gagccgcgat gaccccgttc accacgctcg
      481 agtccaccgt cgtcgtgctg ccgctcgacg acatcgacac cgaccagatc atcccggcgc
      541 ggttcctcca gggcaccgac aaggccgggc tcggcgagca cctgttcgcc gactgcggcc
      601 gccacccgga cttcccgctg aacgagatcg gcgcgcggtt cgcgaagatc ctggtcgccg
      661 gcgcgaactt cgggtgcggc tcgtcgcgcg agcacgcggc gtgggcgctg gtcgaccgcg
      721 gcttccgcgc gatcctcgcg cggtcgttcg gcgacatctt ccgccagaac gcgatcaaga
      781 acggcctcct gccggtcgcg cttcccgccg acgcgcacgc ccgggtggtg gcggcgcgcg
      841 ccgccgaccc gcgcgcgatc gtccgcgtcg acctgccggc gcagcgggtc gcgatcgccg
      901 gggatgccgc ggcatcgttc ggcttcgcga tcgacccgtt cgcccggcac tgcctggtcc
      961 acggggtcga cgagctcggc taccttctcg gattccagga tcggatcacc gagttcgaga
     1021 ggagaccgcg atgaacgcca cgatcgtcct gttgccgggt gatgggatcg gccccgaggt
     1081 ggttcgcgag ggccagcagg tgctcgccgc catcgccgcg cgcgccggcc accagctcgc
     1141 gttcgagtcc cacccgatcg gcggcgccgc gctcaatcag cacggcgatc cgctgccgcc
     1201 ggcgacgctc gaggtgtgca cccgggccga cgcgatcctg ctcggggcgg tcggcggacc
     1261 gaagtgggac gatccgcgcg ccaggcagcg gccggagcag ggcctgctcg gcctgcgcaa
     1321 gcagctcggc ctgtacgcca acctgcgccc ggtgcgcgtc cacccggcgc tggcggccgc
     1381 gtcgccgctc cagcccgcgc tgctcgccgc ggtcgacgtg ctgttcgtcc gcgagctcac
     1441 cggcgggctg tacttcggcc ggccgcggct gcgcgagacc gtcgacggcc ggcgccacgc
     1501 gatcgacacg ctggagtaca gcgatcacga gatcgcgcgc gtcgtgcggc tcgcgttccg
     1561 gctcgcccgc ggccgcaacg gccgggtcac ctcggtcgac aaggccaacg tcctcgagtc
     1621 gtcgcggctg tggcgcgagg tcgccagcga gatcgccgcc gagttccccg acgtcgcgct
     1681 cgagcaccag ctggtcgact cgtgcgcgat gcggatcgtc agcgcgccgc gctcgttcga
     1741 cgtcgtggtc accgagaaca tgttcggcga catcctgacc gacgaggccg cggcgctcgc
     1801 cggctcgctc ggcctgatcc cgagtgcgtc gctcggcgac ggcacgcgcg gcctgtacga
     1861 gccgatacac ggctcggccc ccgatctcgc gggcaaggac gccgcccacc cgatcgggac
     1921 catcctgtcc gcggcgatgc tgctccgcta ctcgctcggc ctggcgcgcg aggccgacct
     1981 cgtcgagcgc gcggtcgcga gcgcgatcgc cgccggagcg cgcaccgccg acatgggcgc
     2041 accgcagggt acagcgctgt cgaccaccgc gatgggggag cggatccggc gcgagatcct
     2101 cgagcgtggg taacgtcacg ccgcgcgccc atgcgatcgc atcggcccgc aggtagagtg
     2161 atcggcatgc gatcctggat ggcgctgctg gcgctcgcgc cggcgtgcgg gttcagctcc
     2221 agcgcgggca gcggcaccgg gaagatcgat gcaggcgatg caggcccggc gtgcttcggc
     2281 gcgttccctc gggtctgctt ccccgcgtcc gcggtgccga tgaagcccag gacgctggac
     2341 ggtctcaccg agatcgacac cgacatgacg gccagcggtt cggcgtgcga tcagaacaac
     2401 gatcagaagg cggcgtactg tgtctacgcc agcgcgggct tcacgctcgc ggccagcgcg
     2461 atgctgaccg cgcacggcag caagccgctc gtggtgctgt ccacgaccat gatggacctc
     2521 gcgggcagca tcgacgtcag cagccacctg gccggagcgc cccagctccg cggggccggg
     2581 gcgaacccgc aggaccccgc ggcgtgcgca ttcaggacgc cacccaccgc ggccgcgatg
     2641 ggaggtg
//
LOCUS       DASVOR010002038         1548 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_400762,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002038 DASVOR010000000
VERSION     DASVOR010002038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1548
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400762"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VF516_37815"
     CDS             <1..250
                     /locus_tag="VF516_37815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEX8113568.1"
                     /translation="THYPLLRDVLGRAARELAQHDITVVDSATAMAEAAREALGSGPN
                     RRGEPGRLDCFATDTSRLDELAPRFLGEAPTGFELVDL"
     gene            complement(266..871)
                     /locus_tag="VF516_37820"
     CDS             complement(266..871)
                     /locus_tag="VF516_37820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113569.1"
                     /translation="MTSIAAESKFVELVRGWLVSLPHDLKIAFDAMDDENLPRPVREV
                     AAGVIAYVVSPNDGVSDRHDAVASYADDVVLLRIALQKALGPGQDEQSFRDRFPELFE
                     GLDESLTLCKAIMGELMTWLESKVPTLPTIEYKGKKITKYLDDDEAREQLFEDGLVFR
                     TDYPVDEKTITDKLKKATTITDVMRRRQAEEARAKGVKARA"
     gene            1156..>1548
                     /locus_tag="VF516_37825"
     CDS             1156..>1548
                     /locus_tag="VF516_37825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113570.1"
                     /translation="MIVSTRKLDYDAGSAEESIKSLMQAQHKAVLKDLRRGAFDDKID
                     SYLGGTPGLLPRGVEEAPERPSEAELAPSTGTVEMLAGPPTEQVLPLIFDELSEPIIL
                     IEEEEPAPTRATPPPLPARLTERHSSLGA"
BASE COUNT          250 a          529 c          519 g          250 t
ORIGIN      
        1 cacgcactac ccgctgctgc gcgacgtgct cggccgcgcc gcgcgcgagc tcgcgcagca
       61 cgacatcacc gtcgtcgatt cggcgacggc gatggccgag gccgcgcgcg aggcgctggg
      121 cagcggcccc aaccgccgcg gcgagccggg ccggctcgac tgcttcgcga ccgacacgtc
      181 gcggctcgac gagctcgctc cccggttcct cggcgaggcc ccgacgggct tcgagctggt
      241 agatctgtag agccgggagg cgggcctacg cgcgggcctt gacgcccttg gcgcgggctt
      301 cctcggcctg gcggcgccgc atcacgtcgg tgatcgtcgt cgccttcttg agcttgtccg
      361 tgatggtctt ctcgtcgacc ggatagtcgg tgcggaacac caggccgtcc tcgaacagct
      421 gctcgcgggc ctcgtcgtcg tcgaggtact tggtgatctt cttgcccttg tactcgatgg
      481 taggcaacgt cggtaccttg ctctccagcc aggtcatgag ctcgcccatg atcgccttgc
      541 agagcgtgag gctctcgtcg agaccctcga acagctcggg gaagcggtcg cggaacgact
      601 gctcgtcctg ccccgggccg agcgccttct gcagcgcgat gcgcaggagg accacatcgt
      661 ccgcgtagct ggcgaccgcg tcgtggcgat ccgacacgcc gtcgttgggt gagacgacgt
      721 aggcgatcac gccggcggcc acctcccgga ccggacgcgg caggttctcg tcgtccatgg
      781 cgtcgaacgc gatcttcagg tcatgaggaa gcgaaacgag ccaaccacgc accaactcga
      841 cgaacttcga ctccgctgca atactcgtca cggggatatg cgtaccccgc gatggcgggg
      901 cctccaaaaa gccagcttcg agactgaagc ccgaactcgg tgagtaccaa ccccgcaggg
      961 gaaatacaag ccagaccgct cggtcagcgc gcggaagccg tcacccatgg taccctgcgg
     1021 gcaccggtgg gcaagcgatc accgatcctt ggctacaatc acaacgtccg gtatcgcggg
     1081 ctggttttcc atgtccagac cgaggattcg ggcgtcgcga gcccgcatct gttcacgcac
     1141 ctcttccatc acggggtgat cgtctcgacg cggaagctgg actacgacgc gggttcggcg
     1201 gaggagtcca tcaagtcgct gatgcaggct cagcacaagg cggtgctgaa ggacctccgg
     1261 cgcggcgcgt tcgacgacaa gatcgactcg tacctcggtg gaactcccgg gctgttgccg
     1321 cgcggtgtcg aggaagcgcc cgagcggccg agtgaggctg agcttgcacc gtcgaccggc
     1381 acggtcgaga tgctggcggg cccgccgacc gagcaggtct tgccgctcat cttcgacgag
     1441 ctcagcgagc cgatcatcct catcgaggag gaggagccgg cgcccacgcg cgcgacgccg
     1501 ccgccgctgc cggcgcggtt gaccgagcgt cacagctcgc tgggcgcg
//
LOCUS       DASVOR010002039         3248 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_459731,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002039 DASVOR010000000
VERSION     DASVOR010002039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3248
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459731"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VF516_37830"
     CDS             complement(<1..732)
                     /locus_tag="VF516_37830"
                     /inference="COORDINATES: protein motif:HMM:NF028481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8113571.1"
                     /translation="MNELTLYQPPTRPWNTPNLSPFCTKLECYLRMAEIPYKTAAMQI
                     GKAPKGKIPYIVLPDGTRMGDSQLIIEHLERALAAEGKPALDGGLSARDRAIGHLVQR
                     TLDEAYYFVTLYTRWQTDDGFAAVRDEFKKFLPGLIIPLIRRDIRKKLQTQGTGRHRL
                     DEAMAFGAADLESCAELLGDQPFLLGDRPRTVDCTLYACLESLLGFPVDSPLKARIAA
                     RGNLVDYRKRIRDRWWKDLERGDAPK"
     gene            complement(817..1200)
                     /locus_tag="VF516_37835"
     CDS             complement(817..1200)
                     /locus_tag="VF516_37835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113572.1"
                     /translation="MRDGDDAPAATAAKPPLKQRLSALFDEYGRIAIITYFTLSILTI
                     IGFSIAIWTGVKPSTETGVIGVIIAGWALGKATLPIRILITLGLTPVVALVVRRRRGL
                     DANEPVEADASADPPASQPPSPGAS"
     gene            complement(1255..>3248)
                     /locus_tag="VF516_37840"
     CDS             complement(1255..>3248)
                     /locus_tag="VF516_37840"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8113573.1"
                     /translation="GPTASPAVAPTPSAPGVNVTLADVGLEAGSLDRTTDPCVDFYQF
                     ACGGWIQNNPIPADRARWSRGSELDERNKALIKTLLEDAAKGATPSPIHKKLGAFYAS
                     CMDEAAIERAGTGAFAPLLAKTRGVKDARTWLTAVVELHKLGISVVWDHHVLPDLKDS
                     TTNLTYLDAGGLGLPDRDYYVKPDFSGKLDGYRAQVGKLLALVPGPGKLDPADVVAIE
                     TELARLTKTAVERRDVAAAYNPTDLKALGRQVRSVDWPAYWKGLGAAPGGKIVLGTPR
                     FFAALDKLRARFKPAQWASYFTYRLVQGLAFAMPRPIDDAAFELTRLLTGVEKQRERG
                     KRCIEHTAAALGELLGQAYVASYFPASSRQGASKLVDALVQAMTEDIAGLDWMTDATK
                     PLALAKLDKIVRMVGYPDHWKTYDFDVKRDDFAGNALRAAAFEVHRELARAGKPVDRS
                     EWQMNVFQVDAYYEPSVNNTALPAGILQPPFFGQDRAVPVNLGGIGMVIGHELTHGFD
                     DQGAQFDGAGNLVNWWQKADLARFADKGKCVADQYSGFEALPRKFIQGQLTLGENIAD
                     LGGVKMAFRAYRALRKDAAKRYVADGFSEDQQFFLAVGQAWCARERPAETERRLTTDP
                     HSPHKFRVFGALRNLPEFAEAFHCVAGTPMRPARTCSVW"
BASE COUNT          447 a         1124 c         1179 g          498 t
ORIGIN      
        1 cttcggcgcg tcgccgcgct ccaggtcctt ccaccagcgg tcgcggatcc gcttgcggta
       61 gtcgaccagg ttgccgcgcg ccgcgatccg cgccttgagc ggcgagtcga ccgggaagcc
      121 cagcagcgat tccaggcagg cgtacagcgt gcagtcgacg gtgcgcggcc ggtcgccgag
      181 caggaacggc tggtcgccga gcagctcggc gcacgactcg aggtcggcgg cgccgaacgc
      241 catggcctcg tcgaggcgat gccggccggt gccctgggtc tgcagcttct tgcggatgtc
      301 gcgccggatc agcgggatga tgaggccggg gaggaacttc ttgaactcgt cgcgcacggc
      361 ggcgaagccg tcgtcggttt gccacctcgt gtacagggtc acgaagtagt aggcctcgtc
      421 gagcgtgcgc tggaccaggt ggccgatcgc gcgatcgcgg gcggacagcc cgccgtcgag
      481 cgccggcttg ccctcggcgg ccagcgcgcg ctccaggtgc tcgatgatca gctgcgaatc
      541 gcccatccgc gtgccgtcgg gcagcacgat gtacgggatc ttgcccttgg gcgccttgcc
      601 gatctgcatc gccgcggtct tgtacgggat ctccgccatc cgcaggtagc actcgagctt
      661 ggtgcagaac gggctgaggt tcggggtgtt ccacggccgg gtcggcggct ggtacagcgt
      721 gagctcgttc atgcggcgac ggtacacccg cgcaggcgcc ggggcgcggc cagcgagcac
      781 tcgccgcgat cggcgcgcct cgcggagcga tcggcgctac gaggcgccgg gcgacggagg
      841 ttgactggcc ggcgggtcgg ccgacgcgtc cgcctcgacc ggctcgttcg cgtccagccc
      901 gcgccgccgc cgcaccacga gcgccaccac cggggtcagc ccgagcgtga tcaggatgcg
      961 gatcggcagc gtggccttgc cgagcgccca gccggcgatg atcacgccga tcacgccggt
     1021 ctcggtcgac ggtttgaccc cggtccagat cgcgatcgag aacccgatga tggtcaggat
     1081 cgacagcgtg aagtacgtga tgatcgcgat ccggccgtac tcgtcgaaca gcgcggacag
     1141 ccgctgcttg agcgggggct tcgccgcggt cgcggccggg gcatcatcgc cgtcgcgcat
     1201 cccggggatg tagcacgccc cggcgcaagg gcacctgcga gccggcgctc accatcacca
     1261 cacggagcag gtccgggccg gacgcatcgg cgtgccggcg acgcagtgaa acgcctcggc
     1321 gaactcgggc aggttgcgca gcgcgccgaa cacccggaac ttgtgcggcg agtgcgggtc
     1381 ggtggtgagc cggcgctcgg tctcggccgg ccgctcgcgg gcgcaccacg cctggccgac
     1441 cgcgaggaag aactgctggt cctcggagaa cccgtcggcg acgtaccgct tggccgcgtc
     1501 cttgcgcagc gcgcgatagg cgcggaacgc catcttgacc ccgcccaggt cggcgatgtt
     1561 ctcgccgagc gtgagctggc cctggatgaa cttcctgggc agcgcctcga accccgagta
     1621 ctggtcggcg acgcacttgc ccttgtcggc gaacctggcg aggtcggcct tctgccacca
     1681 gttcaccagg ttgccggcgc cgtcgaactg cgcgccctga tcgtcgaacc cgtgggtgag
     1741 ctcgtggccg atcaccatgc cgatcccgcc gaggttgacg gggaccgcgc ggtcctggcc
     1801 gaagaacggc ggctgcagga tgccggccgg cagcgcggtg ttgttcacgc tcggctcgta
     1861 gtacgcgtcg acctggaaca cgttcatctg ccactcgctg cgatcgaccg gcttgccggc
     1921 cctggcgagc tcgcggtgga cctcgaacgc ggcagcgcgc agcgcgttgc ccgcgaagtc
     1981 gtcgcgcttg acgtcgaagt cgtaggtctt ccagtgatcg gggtagccga ccatgcggac
     2041 gatcttgtcg agcttggcga gcgcgagcgg cttggtcgca tccgtcatcc agtcgaggcc
     2101 cgcgatgtcc tcggtcatcg cctggaccag cgcgtcgacc agcttgctcg cgccctgcct
     2161 ggagctcgcc gggaagtagc tcgcgacgta ggcctggccg agcagctcgc cgagcgccgc
     2221 cgcggtgtgc tcgatgcacc gcttgccgcg ctcgcgctgc ttctcgaccc cggtcaacag
     2281 ccgggtcagc tcgaacgcgg cgtcgtcgat cggccgcggc atcgcgaacg ccaggccctg
     2341 gaccaggcga tacgtgaagt agctcgccca ctgcgccggc ttgaaccgcg cgcgcagctt
     2401 gtcgagcgcg gcgaagaacc gcggcgtgcc gagcacgatc ttgccccccg gcgcggcgcc
     2461 gagccccttc cagtacgccg gccagtcgac cgagcggacc tggcggccga gcgccttgag
     2521 atcggtcggg ttgtacgccg ccgcgacgtc gcgccgctcg accgcggtct tggtcagccg
     2581 ggcgagctcg gtctcgatcg cgacgacgtc ggcgggatcg agcttgcccg gtcccgggac
     2641 cagcgcgagc agcttgccga cctgagcgcg gtagccgtcg agcttgcccg agaagtcggg
     2701 cttgacgtaa tagtcgcgat cggggaggcc gagccccccg gcatcgaggt aggtcaggtt
     2761 cgtggtcgag tccttgagat cggggagcac gtggtggtcc cacaccacgg agatcccgag
     2821 cttgtggagc tcgaccaccg cggtcagcca ggtccgggca tccttgacgc cgcgggtctt
     2881 ggcgagcagc ggcgcgaacg cgccggtgcc ggcgcgctcg atcgccgcct cgtccatgca
     2941 cgacgcatag aacgcgccga gcttcttgtg gatcgggctg ggcgtcgcgc ccttcgccgc
     3001 atcctcgagc agcgtcttga tcagggcctt gttgcgctcg tcgagctcgc tgccccggct
     3061 ccaccgcgcg cggtccgcgg ggatcgggtt gttctggatc cagccaccgc acgcgaactg
     3121 gtagaagtcg acgcacggat cggtggtgcg atcgagcgag ccggcctcca ggccgacgtc
     3181 ggcgagcgtg acgttgacgc cgggcgcgct cggcgtcggc gccacggccg gcgaggcggt
     3241 gggccccg
//
LOCUS       DASVOR010002040         3288 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_548012,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002040 DASVOR010000000
VERSION     DASVOR010002040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548012"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF516_37845"
     CDS             <1..297
                     /locus_tag="VF516_37845"
                     /inference="COORDINATES: protein motif:HMM:NF016051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEX8113574.1"
                     /translation="PDLLAGLGQARGSRTAPLLVGFAAETRDVIANARAKLAAKRCDL
                     VVANDVGEPGAGFGVDTNRVTVVDHDGAVEIPAGAKAEVAHRILDRVVARLPPR"
     gene            483..851
                     /locus_tag="VF516_37850"
     CDS             483..851
                     /locus_tag="VF516_37850"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX8113575.1"
                     /translation="MRKSLFATLLAIAAMAACKSRSNEPPAADNTAQNERVRAASPTA
                     DNASNSAADRDAMQKIRKAVMDDGSLSTNAHNCKIVVLDGTVTLVGPVASEAERAKVE
                     QIAMANAADRKVVNQLEVTK"
     gene            867..1436
                     /locus_tag="VF516_37855"
     CDS             867..1436
                     /locus_tag="VF516_37855"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HEX8113576.1"
                     /translation="MTKQKEDKKIAVFGLFEDRVALELAIDELRAAGFRESDISALLP
                     DVPSTRQVAHEKHTKAPEGALVGATAGGAIGGTLGLLVGIGALAIPGLGPFIAAGPIM
                     ATLAGAGVGGAVGTLTGTLIGMGIPEYEAKRYESWLHKGGSLLAVHADDGDWAKRAKA
                     VLDRCGAKDIDKASEARGENARDHIHHRA"
     gene            complement(1466..2725)
                     /gene="glyA"
                     /locus_tag="VF516_37860"
     CDS             complement(1466..2725)
                     /gene="glyA"
                     /locus_tag="VF516_37860"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEX8113577.1"
                     /translation="MHPALVATLHAVDPEIADLIAAEERRQRDKIRLIASENYVSGAV
                     LAATGSVLTNKYSEGYPGKRYYEGQQYIDRIEQLCIDRARALFGADHANVQPYSGSPA
                     NLAVFLAFVKPGETVMGMSLPAGGHLTHGWNVSATGTWFRAVQYGVRRTDQRIDLDEV
                     RDLARKERPRLLFAGGTAIPRTIDFAAFAEIAREVGAVFVADIAHIAGLVAGGAHPSP
                     VPVADVVSTTTHKTLRGPRGGLLLCRAAHQKAIDRAVFPGLQGGPHNHTTAGIAVALR
                     EAATGEFQRYAHQIVANARALAAGLVERGWSLVTGGTDNHLILADLTTKQVPGKVAAR
                     ALDAAGIELNYNTVPFDPRKPFDPSGIRLGTPSVTSRGMAEPEMRQIAAWIDAVVSAP
                     GDAALHARIAGEIRELCAKFPAPGILV"
     gene            complement(2759..>3288)
                     /locus_tag="VF516_37865"
     CDS             complement(2759..>3288)
                     /locus_tag="VF516_37865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113578.1"
                     /translation="IAGRLGGPRSLDDRVAASLMVPAVGIALLLPIPLHLPVVALLAD
                     LDFFDVWAMGSLWITGLAHGVFAGLCALRAHDLAAGKRAVSPSAIYFATVLTSCVPVV
                     FLADARVAFVTDMARYVGSEALLVIPPILVAFTALPLVRLLRTLQRLADRERAEIAAA
                     QKLPLAIAVLPRRGT"
BASE COUNT          495 a         1159 c         1225 g          409 t
ORIGIN      
        1 cccgatctgc tcgccgggct cggccaggcg cgcggcagcc gcacggcgcc gctgctggtc
       61 gggttcgccg ccgagacccg cgacgtgatc gccaacgcgc gggccaagct cgccgccaag
      121 cgctgcgacc tggtcgtcgc caacgacgtc ggcgagcccg gcgccgggtt cggggtcgac
      181 accaaccgcg tgaccgtggt cgatcacgac ggcgcggtcg agatccccgc cggcgccaag
      241 gccgaggtcg cccaccgcat cctcgatcgc gtcgtggcgc ggctgccgcc ccggtgagct
      301 cctgcggccc ggcctgagtt gacaggggcg gccggtacga gggtttccga tggcgcgccg
      361 gcgcgctcgg cgtgccatga gcgccaggcg acgcgcacgc gcgatcgtcg cttgctgcga
      421 ttccgacgga cgtcggcgca ctggcatgcg acgtgctctc tccgtatggc gaggagacgt
      481 tcatgcggaa atcactgttc gccaccctgc tcgccattgc ggcgatggcc gcgtgcaagt
      541 cgcgctcgaa cgagccgccg gccgccgaca acaccgcgca gaacgagcgc gttcgcgcgg
      601 cttcgccgac ggccgacaac gcgtccaaca gcgcggccga ccgcgatgcc atgcagaaga
      661 ttcgcaaggc ggtgatggac gacggctcgc tgtcgaccaa cgcccacaac tgcaagatcg
      721 tcgtgctgga cggcaccgtc acgctcgtcg gcccggtcgc gagcgaggcc gagcgcgcga
      781 aggtcgagca gatcgcgatg gccaatgccg ccgatcgcaa ggtcgtcaac caactcgaag
      841 tcaccaagta accaacaggg gacccaatga cgaagcaaaa ggaagacaag aagattgccg
      901 tgttcgggct gttcgaggac cgcgtggccc tcgagctggc gatcgacgag ctgcgcgccg
      961 cggggttccg cgagagcgac atctcggcgc tgctgcccga tgtgccgagc acccgccagg
     1021 tcgcgcacga gaagcacacc aaggcccccg agggcgcgct ggtcggcgcc accgccggcg
     1081 gcgcgatcgg tggaacgctc ggcctgctgg tcggcatcgg cgcgctcgcg atcccgggcc
     1141 tcggaccgtt catcgccgcg ggtccgatca tggccacgct cgccggcgcc ggggtcggcg
     1201 gagcggtcgg aaccctgacc ggcacgctga tcggcatggg gatccccgag tacgaggcca
     1261 agcgctacga gtcgtggctc cacaagggcg gctcgctgct cgcggtccac gccgacgacg
     1321 gcgactgggc caagcgcgcc aaggccgtgc tcgaccgctg cggcgccaag gacatcgaca
     1381 aggcctcgga ggcgcgcggc gagaacgccc gcgaccacat ccaccaccgc gcgtgaccgc
     1441 gcggaccgct ggggcgcgcc gcgagctaga ccaggatccc cggcgccggg aacttcgcac
     1501 acagctcgcg gatctcgccg gcgatccggg cgtgcagcgc cgcgtcgccg ggcgcgctga
     1561 ccacggcgtc gatccacgcc gcgatctgcc gcatctcggg ctcggccatg ccgcggctgg
     1621 tgaccgacgg cgtgccgagc cggatccccg acggatcgaa cggcttgcgc gggtcgaacg
     1681 gcacggtgtt gtagttgagc tcgatgccgg cggcgtcgag cgcgcgggcg gcgaccttgc
     1741 cggggacctg cttggtggtg aggtcggcga ggatcaggtg attgtcggtg ccgccggtga
     1801 ccagcgacca gccgcgctcg accaggccgg ccgccagcgc gcgcgcgttg gcgacgatct
     1861 ggtgggcgta gcgctggaac tcgccggtcg ccgcctcgcg cagcgcgacc gcgatgccgg
     1921 cggtggtgtg gttgtgcggg ccgccctgca gcccggggaa caccgcgcgg tcgatcgcct
     1981 tctggtgggc ggccctgcac agcagcaggc cgccgcgcgg gccgcgcagg gtcttgtggg
     2041 tcgtggtcga caccacgtcg gcgaccggca ccggcgacgg gtgggcgcca ccggcgacca
     2101 ggccggcgat gtgggcgatg tcggcgacga acaccgcgcc gacctcgcgc gcgatctcgg
     2161 cgaacgcggc gaagtcgatc gtgcgcggga tcgcggtgcc gccggcgaac agcaggcgcg
     2221 gccgctcctt gcgcgccagg tcacgcacct cgtcgaggtc gatccgctgg tcggtgcggc
     2281 gcacgccgta ctgcacggcg cggaaccacg tgccggtcgc cgacacgttc cagccgtggg
     2341 tcaggtggcc gccggcgggc aggctcatgc ccatcaccgt ctcgcccggc ttgacgaacg
     2401 cgaggaacac cgcgaggttg gccggcgacc ccgagtacgg ctggacgttg gcgtggtccg
     2461 cgccgaacag cgcccgggcg cggtcgatgc acagctgctc gatccgatcg atgtactgct
     2521 ggccctcgta gtagcgcttg ccggggtagc cctcgctgta cttgttggtg agcaccgagc
     2581 cggtcgcggc gagcacggcg cccgacacgt aattctccga ggcgatcagc cggatcttgt
     2641 cgcgctgccg gcgctcctcg gcggcgatca gatcggcgat ctcgggatcg accgcgtgca
     2701 gcgtggcgac gagggcgggg tgcatgggcg cgtcatagca cgtgcgtccg tagcaggatc
     2761 aggtgccgcg ccgcggcagc accgcgatcg cgagaggcag cttctgtgcg gcggcgatct
     2821 cggcgcgctc gcggtcggcc aggcgctgca gcgtgcgcag cagccgcacc agcggcagcg
     2881 cggtgaaggc gacgaggatc ggcgggatca ccaggagtgc ctcggacccg acatagcgcg
     2941 ccatgtccgt cacgaaggcg acgcgcgcgt cggccaggaa cacgaccggc acgcacgacg
     3001 tgagcacggt cgcgaagtag atcgcggacg gcgagaccgc gcgcttgccg gccgcgagat
     3061 cgtgggcccg cagcgcgcag aggcccgcga acaccccgtg cgccaggccg gtgatccaca
     3121 ggctgcccat cgcccagacg tcgaagaagt cgagatcggc gagcagcgcg acgaccggga
     3181 ggtggagcgg gatcggcaag agcagcgcga tgccgaccgc cggcaccatc aggctggcgg
     3241 cgacccggtc gtcgagcgac cgcgggccgc cgagccgccc ggcgatcg
//
LOCUS       DASVOR010002041         1518 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_517991,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002041 DASVOR010000000
VERSION     DASVOR010002041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1518
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517991"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /locus_tag="VF516_37870"
     CDS             <1..631
                     /locus_tag="VF516_37870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113579.1"
                     /translation="PLRDGMRATVQVLGSDAPVAGTVVRVAPAVDPTTLLGMVRIALA
                     SAEGIQVGTAATARISIGQRPGVRVPASALRRSLVGEDEVVVCDGGTAHVRKVAIGNR
                     GDQGVEIKDGIKAGERVVVDHVLGLQDEQPLTAPGQATDDKKDDKKAGKAKDDDDKDD
                     DKKPGDRKADDQKAKAVKTTSPQGDGTGKASDDKAAPAPGSAGKGGGEK"
     gene            628..>1518
                     /locus_tag="VF516_37875"
     CDS             628..>1518
                     /locus_tag="VF516_37875"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8113580.1"
                     /translation="MKLVAWLRRHASLVWIAVLGLAATGAVSIFQLPSGIYPEMQFPR
                     VIVVAKAGQLSPDLVEAQMTRPLEQALAIVPGIRHVRARTIRGAVELSLLLTDGTDPL
                     TAQYACQAAVDHVELPRGATTVVQRVLPTSVPVITFNVVAPKGAATDLRRLREVAERI
                     LRPAIVRVPGVGGVEVAGGRVRQIQLLIHPAELAALHLTPAQLAQKIDAEDQVIAAGR
                     VWDQHQTLPVVYDAQAADLEQLRQLPIASGPTGPVPLSAVADVRDGNEDPDVIIAGGR
                     GEAVAVSVARLPGASTPAVVD"
BASE COUNT          226 a          558 c          564 g          170 t
ORIGIN      
        1 gccgctgcgc gacggcatgc gcgccaccgt ccaggtgctc ggctccgacg cgccggtcgc
       61 ggggaccgtg gtgcgcgtcg cgcccgcggt cgatccgacg acgctgctcg ggatggtccg
      121 catcgcgctg gccagcgccg agggcatcca ggtcgggacc gcggcgaccg cccggatctc
      181 gatcggacag cgcccgggcg tgcgcgtccc ggcctcggcg ctgcgccgct cgctggtcgg
      241 cgaggacgag gtcgtggtgt gcgacggcgg caccgcgcac gtccgcaagg tcgcgatcgg
      301 caaccgcggc gatcagggcg tcgagatcaa ggacgggatc aaggccggcg agcgcgtcgt
      361 cgtcgaccac gtgctcggcc tccaggacga gcagccgctc accgcgcccg gccaggctac
      421 cgacgacaag aaggacgaca agaaggccgg caaggcgaaa gacgacgacg acaaagacga
      481 cgacaagaaa cccggcgaca ggaaggccga cgaccagaag gccaaggccg tgaagaccac
      541 gagcccccag ggcgatggca ctggcaaggc cagcgacgac aaggccgcac ctgcacccgg
      601 cagcgccggg aaaggcggcg gcgagaaatg aagctcgtgg cctggctccg ccggcacgcg
      661 agcctggtgt ggatcgccgt gctcgggctc gcggcgaccg gcgcggtctc gatcttccag
      721 ctgccgagcg ggatctaccc cgagatgcag ttcccgcggg tgatcgtcgt cgccaaggcc
      781 ggccagctgt cgccggacct cgtcgaggcc cagatgaccc ggccgctcga gcaggcgctc
      841 gcgatcgtgc ccgggatccg ccacgtccgc gcccgcacga tccgcggcgc ggtcgagctg
      901 tcgctgctgc tcaccgacgg caccgatccg ctgaccgcgc agtacgcgtg ccaggccgcg
      961 gtcgatcacg tcgagctgcc gcgcggcgcg accaccgtcg tgcagcgcgt gctgccgacc
     1021 tcggtgccgg tgatcacgtt caacgtcgtc gcgccgaagg gcgccgccac cgacctgcgg
     1081 cggctgcgcg aggtcgccga gcgcatcctc cggccggcga tcgtccgggt gcccggcgtc
     1141 ggcggcgtcg aggtcgcggg cggccgcgtc cggcagatcc agctgctgat ccacccggcg
     1201 gagctcgcgg cgctccacct cacgccggcg cagctcgccc agaagatcga tgccgaggac
     1261 caggtgatcg cggcgggccg ggtgtgggac cagcaccaga ccttgccggt cgtgtacgac
     1321 gcccaggccg ccgatctgga acagctgcgc cagctgccga tcgccagcgg cccgaccggc
     1381 ccggtcccct tgtccgcggt cgccgacgtg cgcgacggca acgaggaccc cgacgtgatc
     1441 atcgcgggcg ggcgcggcga ggcggtcgcg gtctcggtcg cccggctgcc cggcgcgagc
     1501 acgccggcgg tggtcgac
//
LOCUS       DASVOR010002042         2982 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_342612,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002042 DASVOR010000000
VERSION     DASVOR010002042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2982
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342612"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..948)
                     /locus_tag="VF516_37880"
     CDS             complement(142..948)
                     /locus_tag="VF516_37880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8113581.1"
                     /translation="MTRIVAVANQKGGVGKTTTAINLGASVASRGYRVLLADFDPQGN
                     ASSGIGYTRDRIELSIYDALTGEVAMEDVIRPTEITTMFVAPATTDLVGAEIELIGAE
                     HRERYLADALAAVAGGYDYVFIDCPPSLGLLTLNALVAAEGVIIPMQAEYFALEGLSA
                     LTATIEKVRAAYNPKLAIDGVVFTMYDARLSVAVQVKTEVSRYFGDKVFTEMVPRNVR
                     LSEAPSHGKPALLYDLRCQGTKSYLALADEFLDRTLMIDVPPPRAEVSSS"
     gene            1125..1307
                     /locus_tag="VF516_37885"
     CDS             1125..1307
                     /locus_tag="VF516_37885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113582.1"
                     /translation="MSSGIPRIYTSFAEFEREELRRLDSLHTSVDDLVEERFAEDLEF
                     GGGDRPRKRGRPRKNP"
     gene            1774..>2982
                     /locus_tag="VF516_37890"
     CDS             1774..>2982
                     /locus_tag="VF516_37890"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113583.1"
                     /translation="MLGRSLWNYDVVSQIGEGGMGVVYLARHAMLGRAVAIKVLHSAR
                     SDGDLVARFFNEARAATAVRHPAIVDVYDVGFLEDRRAYIVMEYLDGESLAARIRRGR
                     ATIEATLTIVRALARALQAVHARGIVHRDLKPDNVFLVPDPETPDRERVKILDFGIAK
                     LTEPAGSLLPTTTGTLLGTPIYMSPEQCRGDRTIDHRTDLYSLGCVAYEMLCGQPPFP
                     GDAPGDVIARHQYVEPAPLRWLRSDVPAGVERLVMRLLEKRPGNRYRTAADVVRAIDG
                     LLAPVAVADLLPTLSTTMDATAATVAGKPPAAPRRRHLGLLAGAATAALALLVLLLLH
                     PAGVPDACEPGAPDDHLSGAEDLSGRAERARAAARRFASEVALRQRGWLQLARCVDTL
                     LPWHRGGELAR"
BASE COUNT          435 a         1055 c         1025 g          467 t
ORIGIN      
        1 ggtgcggcgc cggctgcgat cgcgcgtcga cgaccgcagc caccgccgac ggcggcggcc
       61 cacccggcag cagcgccgac agcccgcggc cgaggccacg cttcttcaca tcgctcatca
      121 tggacctcgt gggatcgacg ctcacgagga cgatacctcg gcccgcggcg gcggcacgtc
      181 gatcatcagc gtccggtcga ggaactcgtc ggcgagcgcg aggtagctct tggtgccctg
      241 gcagcgcagg tcgtagagca gcgccggctt gccgtggctg ggcgcctcgg acagccggac
      301 gttgcgcggc accatctcgg tgaacacctt gtcgccgaag taccgggaga cctcggtctt
      361 gacctgcacc gcgacgctga gccgcgcgtc gtacatcgtg aacaccaccc cgtcgatcgc
      421 cagcttgggg ttgtacgccg cgcgcacctt ctcgatcgtc gcggtcagcg ccgacaggcc
      481 ctcgagcgcg aagtactcgg cctgcatcgg gatgatcacg ccctcggccg cgaccagcgc
      541 gttgagcgtg agcaggccga gcgacggcgg gcagtcgatg aacacgtagt cgtagccgcc
      601 ggccaccgcg gccagcgcgt ccgccaggta gcgctcgcgg tgctccgcgc cgatcagctc
      661 gatctcggcg ccgaccaggt cggtggtcgc cggcgcgacg aacatcgtcg tgatctcggt
      721 cggccggatc acgtcctcca tcgcgacctc gccggtcagc gcgtcgtaga tcgacagctc
      781 gatccggtcg cgcgtgtagc cgatgcctga cgacgcgttg ccctgcggat cgaagtcggc
      841 gagcaggacg cggtagccgc gcgacgccac cgacgccccg aggttgatcg cggtcgtggt
      901 cttgccgact ccgccttttt ggttcgcgac cgcgacgatc cgggtcatgg ctgccgaggt
      961 accacgcggc tatgacgatc cccagaaacc ggcctcccat tgcgccgctg cggccgctcg
     1021 cgatcgcgcg cgcgtttcgc gaaaaaaaac ttggctcacg aacctcgcct ccgtaggttg
     1081 tagacacatg taggacatgt gggatgtcct cgaaggagcc gccaatgtca tctgggattc
     1141 caaggattta cacttcgttt gccgagttcg agcgcgagga gctgcggcgc ctggattcac
     1201 tccacacctc cgtcgacgac ctggtcgagg agcggttcgc cgaggacctc gagttcggcg
     1261 gcggcgaccg cccgcgcaag cgcggccggc cgcgcaagaa cccgtagccg cgagcggtcg
     1321 gccggcagcg gccggcagcg gtcagcggtc gttcgacgcg ccacgcatcg cgcggccggt
     1381 gcgtaggtcg caacgcagct cctgcgaacg cgccggtgca gtgcgaatcg acgagcgcgg
     1441 acgacggtcc gctggtcgga caaaccagcc agtgcggtga gagctggctt ccgtgatgcg
     1501 agcgcacgat tgcagcgcgc atgcgcgatc gcgactcccg gcgtgccagt gcgcgtgatg
     1561 cagatttcga tcgctgcgca tcatgcgcag cgccgcacgg gccgcggctg tcgacgcacg
     1621 acgtcgaatg gacgtggtct ctcgcgtgca catccgctcc gcgcttagtc gtagcttcgt
     1681 cctgcgtcac gacatggcgg acagcgccgg gggcacgcgc agcatcccgc tgcgtgcgca
     1741 tgttcccgtc gtggcgccgc acgggaggat cgcatgctgg gccgaagcct gtggaactac
     1801 gatgtcgtct cgcagatcgg agagggtgga atgggcgtgg tctacctggc gcggcacgcg
     1861 atgctcggac gcgccgtcgc gatcaaggtg ctgcactccg cgcggtcgga cggcgacctc
     1921 gtggcccggt tcttcaacga agcgcgcgcg gcgaccgcgg tccgtcaccc ggcgatcgtc
     1981 gacgtctacg atgtcggctt cctcgaggat cgccgggcgt acatcgtcat ggagtacctc
     2041 gacggcgaga gcctggcggc gcggatccgg cggggccgcg cgacgatcga ggcgacgctg
     2101 accatcgtcc gcgcgctcgc gcgtgcgctg caggcggtgc acgcgcgcgg catcgtccat
     2161 cgcgatctca agccggacaa tgtgttcctg gtccccgatc ccgagacgcc ggaccgcgag
     2221 cgcgtgaaga tcctcgactt cggcatcgcg aagctcacgg agccagcggg tagcctcctt
     2281 ccgacgacca ccggcacgct gctgggaact ccgatctaca tgtcccccga gcaatgccgc
     2341 ggagaccgca cgatcgacca ccgcactgat ctgtactccc tcggctgcgt ggcctacgag
     2401 atgctgtgcg ggcagccgcc gttccccggc gatgctcccg gcgacgtgat cgcgcgccat
     2461 caatatgtcg agcccgcgcc gctgcgctgg cttcgatcgg atgttccggc cggcgtcgag
     2521 cgactcgtga tgcgattgct cgagaagcgg ccggggaatc gctaccgcac cgccgcggac
     2581 gtcgttcgcg cgatcgatgg cctgctggcg ccggtcgccg tcgccgatct cctgccgacg
     2641 ctgtccacga cgatggatgc aaccgcggcg acggtcgctg gcaagccacc ggcagcgccc
     2701 cgccgccgcc acctcggcct cctcgcgggc gcggcgaccg ccgccctcgc gctgctcgtc
     2761 ctgctgttgc ttcaccccgc gggcgtaccc gacgcctgtg aaccaggtgc gccggacgac
     2821 cacctttcgg gcgccgagga tctgtccggg cgagccgagc gggcgcgtgc cgccgcgcgc
     2881 cggttcgcgt ccgaggtcgc gctgcgccag cgcggatggc ttcagctcgc gcgctgcgtc
     2941 gatacgctgc ttccctggca tcgcggtggt gaactcgcca gg
//
LOCUS       DASVOR010002043         4549 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_578235,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002043 DASVOR010000000
VERSION     DASVOR010002043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578235"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VF516_37895"
     CDS             <1..601
                     /locus_tag="VF516_37895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8113584.1"
                     /translation="VRRHPYAVILLDEMEKAHADVWSVLLQVLDDGRLTDGQGRTVDF
                     RNTVVIMTSNVGSAHLLKLTRDNREQIEKLVMRELSTTFRPEFLNRIDEVIFFNPLGE
                     AQLAQIVRIQVERFQKLLAERQITLHLTDQAVARVAEAGYDPVYGARPLKRALQKLVI
                     DPLASRLLAGDFQPGDQIEAATEGDRIVFHRVAPKGAAA"
     gene            605..1390
                     /locus_tag="VF516_37900"
     CDS             605..1390
                     /locus_tag="VF516_37900"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEX8113585.1"
                     /translation="MPQQIDFDPSVDYYKALGVSQSASAEEIKRAYRKLAKANHPDST
                     GGDKAKESRFKDISNAYDVLGDAKKRAMYDEIRAGGGLPRGFPGGYTYSAQGGGPGVF
                     DLGDLFSQFFGSSAAGAGRGGRVHVEHMDFDEPRPRGRQRRREAHDAEFEHVKASDGS
                     WLRASSGHDVASDVRIAFDRAILGTVATVATIEGKAEVKIPPGTSSGKKLRLRGKGMP
                     DRAGGHGDHYVTVQIDVPHELDDEAKKLLVQLATRVRKRERND"
     gene            1413..3866
                     /gene="lon"
                     /locus_tag="VF516_37905"
     CDS             1413..3866
                     /gene="lon"
                     /locus_tag="VF516_37905"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEX8113586.1"
                     /translation="MTRHIETAGQYPVIPLRTEVQLPGHVGPLEIGREASVRAIEAAT
                     RDDNLIVIIPQKNPAVRDPGQRDLHEVGVRAEIVQVVKHSPGRFTCVMRFLERVHIDA
                     LVATEPFLIASVSALQSTSSASAEHLISTTAKVRDYLLAVVTDAQAKESKPDSGRETK
                     EAKEPRGELTRAQVQAIVDPDKLVDAAAPYLELERDDLTSLLIETDTMKRLERIIPSL
                     ERQATVLRLKADIGAELEGESSRTHRERVLRDRMRQIQEELGEQDDNAEIDELRKKIE
                     DSKMSDEVRSVAKKQLSRMSQMASSSPEYNIARTYVENLLEIPWNVFTEDRLDVSAAR
                     AILEAEHSGLDKVKRRILEFLAVRKLAPNKHGPILLLVGPPGVGKTSLGKSIASSLGR
                     KYVRVSLGGVRDEAEVRGHRRTYIGALPGRIVAGLKKAGSMNPVFVLDEIDKLAADMR
                     GDPAAAMLEVLDPEQNKDFVDHYVEVPVDLSKVMFICTANQTETISQPLLDRMEMVEL
                     SGYTSVEKLAIAKNHLLLKQLGEHGLAKGQLEIEDEVLIEIIHNYTREAGVRNLEREI
                     AAVCRGAAVRIAEGATQIHFDRGQLEDLLGPPRHVSDTAGRKPEVGVITGLAWTPVGG
                     DIMFIECRIYPGKGEVKLTGQMGDVMKESAQAAYSWTRSNAVRLGIDPDKLANSDLHI
                     HLPQGAIKKDGPSAGVALTVAIVSVFTGRPVRHDVAITGEIDLRGHALPVGGIKEKVI
                     AAHRAGIDIVFLPERNLKDTIDIPDEVKRELDIRTMTKIDDALQVALGDAPATPEPEP
                     AIPPPAAPSRGNLGDRLPS"
     gene            complement(3878..>4549)
                     /locus_tag="VF516_37910"
     CDS             complement(3878..>4549)
                     /locus_tag="VF516_37910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX8113587.1"
                     /translation="ENTEDLYAGIEHYVDPRRTAAESIAIITRFGSERVIVYAFEYAR
                     RHGRREVTLVHKANILKMSNGLFLDTGREVARRYPDIEFEDMIVDATAMKLVIAPERF
                     DVIVTMNLFGDILSDLAAGLIGGLGVAPAANIGDSGCAMFEAVHGTAPDIAGQGIANP
                     TGLMLSAAMLLDHVGQPGAAQRLRAGIAAALAHTETRTGDLGGRASTALFTDAVIRAM
                     VAGAA"
BASE COUNT          789 a         1634 c         1525 g          601 t
ORIGIN      
        1 ggtccgccgc cacccctacg cggtgatcct gctcgacgag atggagaagg cgcacgccga
       61 cgtgtggagc gtgctgctcc aggtgctcga cgacggccgg ctgaccgacg gccagggccg
      121 cacggtcgac ttccgcaaca ccgtggtcat catgaccagc aacgtcggct cggcccacct
      181 gctcaagctg acccgggaca accgcgagca gatcgagaag ctggtgatgc gcgagctgtc
      241 gacgacgttc cggcccgagt tcctgaaccg gatcgacgag gtgatcttct tcaacccgct
      301 cggcgaggcc cagctcgccc agatcgtgcg gatccaggtc gagcggttcc agaagctgct
      361 ggccgagcgg cagatcacct tgcacctcac cgaccaggca gtcgcccgcg tggccgaagc
      421 tggctacgat ccggtgtacg gtgcgcgccc gctcaagcgc gccctccaga agctcgtgat
      481 cgacccgctc gccagccgcc tcctcgccgg ggacttccag ccgggcgacc agatcgaagc
      541 cgccaccgag ggggatcgca tcgtgttcca ccgcgtcgca ccgaagggag cagccgccta
      601 agccatgccg cagcagatcg acttcgatcc gagcgtcgac tactacaagg ccctgggcgt
      661 cagtcagtcc gcgagcgccg aggagatcaa gcgcgcctac cgcaagctcg ccaaggcgaa
      721 ccatccggat tccaccggcg gcgacaaggc gaaggagtcg cggttcaagg acatctccaa
      781 cgcgtacgac gtgctcggcg acgccaagaa gcgcgcgatg tacgacgaga tccgcgccgg
      841 cggcggcctg ccgcgcgggt tccccggcgg ctacacgtac agcgcgcagg gcggcggacc
      901 cggcgtgttc gacctcggcg acctgttcag ccagttcttc ggcagcagcg ccgccggggc
      961 cgggcgcggc ggccgggtcc acgtcgagca catggacttc gacgaacccc gcccgcgcgg
     1021 ccggcagcgg cggcgcgaag cgcacgacgc cgagttcgag cacgtcaagg ccagcgacgg
     1081 cagctggctg cgggcctcca gcggccacga cgtcgcgtcc gatgtccgga tcgcgttcga
     1141 tcgcgcgatc ctcggcacgg tcgccaccgt cgcgacgatc gaaggcaagg ccgaggtgaa
     1201 gatcccaccc ggcacgtcga gcggcaagaa gctgcgactg cgcggcaagg gcatgcccga
     1261 ccgcgccggc ggccacggcg accattacgt gaccgtccag atcgatgttc cccatgagct
     1321 cgatgacgag gccaagaaac tcttggtcca gctcgcaact cgcgtgcgca aacgcgagcg
     1381 caacgactga aacgtcgtag actgaaggag tcgtgaccag acacatcgag accgccggtc
     1441 agtaccccgt cattccgctc cggaccgagg tgcagctccc cggccacgtc ggcccgctcg
     1501 agatcgggcg cgaggcgtcg gtccgcgcga tcgaggccgc gacccgcgac gacaacctga
     1561 tcgtcatcat cccgcagaag aaccccgcgg tccgcgatcc cggccagcgc gatctgcacg
     1621 aggtcggcgt gcgcgccgag atcgtccagg tcgtcaagca ctcgcccggc cggttcacct
     1681 gcgtgatgcg gttcctcgag cgcgtccaca tcgacgcgct cgtcgccacc gagccgttcc
     1741 tgatcgcgag cgtgtcggcg ctgcagtcga cgtcgagcgc gtcggccgag cacctgatct
     1801 ccaccaccgc caaggtccgc gactacctgc tcgcggtcgt caccgacgcc caggccaagg
     1861 agtccaagcc ggactccggc cgcgagacca aggaggccaa ggagccgcgc ggcgagctga
     1921 cccgggccca ggtccaggcg atcgtcgacc cggacaagct ggtcgacgcc gccgcgccgt
     1981 acctcgagct cgagcgcgac gacctgacca gcctcctgat cgagaccgac acgatgaagc
     2041 ggctcgagcg gatcatcccg tcgctcgagc gccaggccac cgtgctccgg ctcaaggccg
     2101 acatcggcgc cgagctcgag ggcgagtcgt cgcgcaccca ccgcgagcgc gtgctgcgcg
     2161 accggatgcg ccagatccag gaggagctcg gcgagcagga cgacaacgcc gagatcgacg
     2221 agctgcgcaa gaagatcgag gacagcaaga tgagcgacga ggtccgctcg gtcgccaaga
     2281 agcagctgtc gcggatgagc cagatggcgt cgtcgtcgcc cgagtacaac atcgcgcgga
     2341 cctacgtcga gaacctgctc gagatcccgt ggaacgtgtt caccgaggac cggctcgacg
     2401 tgtcggccgc gcgcgcgatc ctcgaggccg agcactccgg gctcgacaag gtcaagcgcc
     2461 ggatcctcga gttcctcgcg gtccggaagc tcgcgcccaa caagcacggg ccgatcctgt
     2521 tgctcgtcgg gccgcccggc gtcggcaaga cctcgctcgg caagtcgatc gcgtcgtcgc
     2581 tcggccgcaa gtacgtgcgg gtctcgctcg gcggcgtgcg cgacgaggcc gaggtccgcg
     2641 gccatcgccg gacctacatc ggcgcgctgc ccggccggat cgtcgccggg ctcaagaagg
     2701 ccggctcgat gaacccggtg ttcgtgctcg acgagatcga caagctcgcc gccgacatgc
     2761 gcggcgatcc ggccgccgcg atgctcgagg tgctcgatcc cgagcagaac aaggacttcg
     2821 tcgaccacta cgtcgaggtc ccggtcgacc tgtccaaggt gatgttcatc tgcaccgcca
     2881 accagaccga gacgatcagc cagccgctgc tcgaccgcat ggagatggtc gagctgtcgg
     2941 gctacaccag cgtcgagaag ctggcgatcg ccaagaacca cctgttgctc aagcagctcg
     3001 gcgagcacgg cctggccaag ggccagctcg agatcgagga cgaggtcctc atcgagatca
     3061 tccacaacta cacccgcgag gccggcgtcc gcaacctcga gcgtgagatc gccgcggtgt
     3121 gccgtggcgc cgcggtgcgg atcgccgagg gcgccacgca gatccacttc gaccggggcc
     3181 agctcgagga cctgctcggc ccgccgcgcc acgtcagcga caccgcgggc cgcaagcccg
     3241 aggtcggcgt catcaccgga ctggcgtgga cgcccgtcgg cggcgacatc atgttcatcg
     3301 agtgccggat ctaccccggc aagggcgagg tcaagctcac cggccagatg ggcgacgtga
     3361 tgaaggagag cgcccaggcg gcgtactcgt ggacgcggtc caacgcggtc cggctcggca
     3421 tcgatccgga caagctcgcc aacagcgacc ttcacatcca cctgccgcag ggcgcgatca
     3481 agaaggacgg gccgtccgcc ggcgtcgcgc tgaccgtggc gatcgtgtcg gtgttcaccg
     3541 gccggccggt gcgccacgac gtcgcgatca ccggcgagat cgacctccgc ggccacgcgc
     3601 tgccggtcgg cggcatcaag gagaaggtga tcgccgcgca ccgcgccggc atcgacatcg
     3661 tgttcctgcc cgagcgcaac ctcaaggaca ccatcgacat ccccgacgag gtcaagcgcg
     3721 agctcgacat ccgcacgatg accaagatcg acgacgcgct ccaggtcgcg ctcggcgacg
     3781 cgccggcgac ccccgagccc gagcccgcga tcccgccgcc ggcggcaccg tcacgcggca
     3841 acctgggcga ccgcctgccg agctgaaagc aggctgttca ggcggcgccg gcgaccatcg
     3901 cgcggatcac cgcgtcggtg aacagcgcgg tgctggcgcg gccgccgaga tcaccggtcc
     3961 gggtctcggt atgcgcgagc gccgcggcga tcccggcgcg cagccgctgg gccgccccgg
     4021 gctggccgac gtgatcgagc agcatcgccg ccgacagcat cagcccggtc gggttggcga
     4081 tgccctggcc cgcgatgtcg ggcgccgtgc cgtgcaccgc ctcgaacatc gcgcagccgc
     4141 tgtcgccgat gttggccgcc ggcgcgacgc cgagcccgcc gatcagcccg gcggccagat
     4201 cggacaggat gtcaccgaac aggttcatcg tgacgatcac gtcgaatcgc tcgggcgcga
     4261 tcaccagctt catcgcggtg gcgtcgacga tcatgtcctc gaactcgatg tcggggtagc
     4321 gcctggcgac ctcgcggccg gtgtcgagga acaggccgtt ggacatcttg aggatgttcg
     4381 ccttgtggac cagcgtgacc tcgcgccggc cgtggcgccg cgcgtactcg aacgcgtaca
     4441 cgatcacgcg ctcgctgccg aaccgggtga tgatcgcgat cgactccgcc gcggtccgcc
     4501 gcggatcgac gtagtgctcg atccccgcgt agaggtcctc ggtgttctc
//
LOCUS       DASVOR010002044         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_459777,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002044 DASVOR010000000
VERSION     DASVOR010002044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_459777"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="VF516_37915"
     CDS             complement(<1..1163)
                     /locus_tag="VF516_37915"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX8113588.1"
                     /translation="MGDAQGTTLSFHEDGKSGRIGPRPEPVLVLALECGRPRAGSVRY
                     RLADLSSVTLGRGPERRAERVGTELTIRVPDKWMSSRHARIEPSFGRWVLTDTESKNG
                     SIVDGHTTKRAVLTDGSLIELGHTLFLFFERLPVEAEAPPLLELVPSDGPPGFATLMP
                     MWNLELTRLRQIASSEIPMLIEGESGTGKEVIARAIHDQSGRRGAFVPVNCGALPENL
                     VESELFGYKKGAFSGAQNDHPGLVRTADAGTLFLDEIGDLPASSQAALLRVLQEREVM
                     PVGGTRPVEIDLRVVAATHRDLDDMVAEGSFRHDLFARLAGFRVAVPPVAERRTDLGV
                     LIGVLHGRIFPAEHPGFDIDAARLLLRDPWPLNVRELEQALATALVLAGAEPVR"
     gene            1228..1791
                     /locus_tag="VF516_37920"
     CDS             1228..1791
                     /locus_tag="VF516_37920"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113589.1"
                     /translation="MGDLLTFAPGQVRTLGPRPGSRCGLRRGTSATRWYELGCRLEAS
                     EPARAIAAYERAVAGCPGFADAHNNLGRLLHDAGSLGAAESSYRLAICADPGIALYWF
                     NLGVAVEDQGRRAEAIAAYERAIEISERSEAAGGGDPAGVADAHYNLARLLELTGRRT
                     GDELMLRRAVRHLLRYRELTRRNARAH"
     gene            complement(1858..>2256)
                     /locus_tag="VF516_37925"
     CDS             complement(1858..>2256)
                     /locus_tag="VF516_37925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113590.1"
                     /translation="LAFAAAHHVADPARHTFLTADVFAHLPAYAGEPFDLVIIDPPSM
                     TSQASQVPSVLAAYRKLYRAAARQVRPGGALIAACCTSRIERAQFHQTVRDALGPRFA
                     LDHEIPPEPDHPVGFPQADYLKIGWWKLAA"
BASE COUNT          322 a          789 c          845 g          300 t
ORIGIN      
        1 cgcaccggct cggcgccggc gagcaccagc gcggtcgcga gcgcctgctc gagctcgcgg
       61 acgttgagcg gccacgggtc gcgcagcagc aggcgggcgg cgtcgatgtc gaacccgggg
      121 tgctcggcgg ggaagatccg cccgtggagc acgccgatca gcacgccgag atcggtgcgg
      181 cgctcggcga ccggcggcac ggcgacgcgg aacccggcga gccgggcgaa caggtcgtgg
      241 cggaagctgc cctcggcgac catgtcgtcg agatcgcggt gcgtcgcggc gacgacccgg
      301 aggtcgatct cgaccggccg cgtgccgccg accggcatca cctcgcgctc ctgcagcacg
      361 cgcaacagcg cggcctgcga gctcgccggc aggtcgccga tctcgtcgag gaacagcgtg
      421 ccggcgtcgg cggtgcgcac cagccccggg tggtcgttct gggcgccgct gaacgcgccc
      481 ttcttgtagc cgaacagctc gctctcgacg aggttctccg gcagcgcgcc gcagttgacc
      541 ggcacgaacg cgccgcggcg acccgactgg tcgtggatcg cgcgcgcgat cacctccttg
      601 ccggtgccgc tctcgccctc gatgagcatc gggatctccg acgatgcgat ctggcgcagc
      661 cgggtcagct ccaggttcca catcggcatc agggtcgcga acccgggcgg gccgtcgctc
      721 ggcaccagct cgagcagcgg cggcgcctcg gcctcgaccg gcagccgctc gaagaacagg
      781 aacagcgtgt gcccgagctc gatcagcgag ccgtcggtca gcaccgcgcg cttggtggtg
      841 tggccgtcga cgatcgagcc gttcttcgac tcggtgtcgg tcagcaccca gcgcccgaag
      901 ctcggctcga tccgggcgtg gcgcgacgac atccacttgt ccggaacgcg gatcgtcagc
      961 tcggtcccga cgcgctcggc gcggcgctcg gggccgcggc cgagcgtgac cgagctcaga
     1021 tcggccaggc gatagcgcac cgagccggcg cgtggccggc cgcactcgag cgccaggacc
     1081 aggactggct ccggcctggg accgatccgg ccggacttgc catcttcgtg gaagctaagg
     1141 gtcgtgccct gcgcgtcacc cacagcggtc gcatcttaac atcgctcggg agcgagattg
     1201 tcagagggtt ccgatagcgt tgcatacgtg ggtgatcttc tgacctttgc acccggccaa
     1261 gtacggacgc tcgggccgcg ccccgggtcg cggtgcggcc tccgccgcgg cacgagtgcg
     1321 acccgctggt acgagctggg ctgccgcctg gaggccagcg agccggcgcg cgcgatcgcc
     1381 gcctacgagc gggccgtcgc cggctgcccg ggcttcgcgg acgcgcacaa caacctcggg
     1441 aggctgcttc acgacgccgg ttcgctgggc gcggccgagt cgagttaccg gctcgccatc
     1501 tgcgccgatc ccggcatcgc cctgtactgg ttcaacctcg gcgtcgccgt cgaggatcag
     1561 ggccggcggg ccgaggcgat cgcggcctac gagcgcgcga tcgagatctc cgagcgcagc
     1621 gaggcggcag ggggtgggga cccggccggc gttgccgacg cgcactacaa cctcgcccgg
     1681 ctgctcgagc tgaccgggcg gcggacggga gacgagctca tgctccgccg cgccgtccgg
     1741 cacctgctgc gctaccgcga gctgacccgg cgcaacgcgc gggcgcactg agccgggctc
     1801 ggggccgggg tggcgtcgca tgagacaggg gtgcgacgat tccggacgtc gcagtggtca
     1861 cgcggcgagc ttccaccagc cgatcttcag gtaatcggcc tgcggaaatc cgaccgggtg
     1921 atcgggctcc ggcgggatct cgtggtcgag ggcgaagcgc gggccgagcg cgtcgcgaac
     1981 ggtctggtgg aactgcgcgc gctcgatccg gctcgtgcag caggcggcga tcagcgcgcc
     2041 accggggcgg acctggcgag cggccgcgcg gtacagcttg cgatacgcgg ccagcaccga
     2101 cgggacctgc gacgcctggc tggtcatcga cggcggatcg atgatcacca gatcgaacgg
     2161 ctcgccggca taggccggga gatgcgcgaa cacatcggcg gtgaggaacg tgtgccgcgc
     2221 cggatcagcg acgtggtggg cggcagcgaa cgcgag
//
LOCUS       DASVOR010002045         2856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_430150,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002045 DASVOR010000000
VERSION     DASVOR010002045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430150"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="VF516_37930"
     CDS             complement(<1..815)
                     /locus_tag="VF516_37930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113591.1"
                     /translation="MRTAIACGAFVVLVGCGGNGPDINTTQDNVCDQIAEVACFDMYQ
                     CCSEGEIERTLMVSDPRTQAECQSDLATRCRRQAADLDFSIKNKHVKFDAKVMNDCLK
                     EFVAPSNTCVTVEAVKPWAMACVTSAWTGIVEAGGACDFGYECAKDNFCASNRTCVAL
                     PTESMPCSVQGCASGLFCDTAAVGGPVCRALLGAGVACTSTPQCQKGLFCDPAAPVGM
                     RTCTPLLANGATCSGNTTCSSNLCLIGTCSVTGSSCFTDASCGGRCSNNPNIS"
     gene            complement(934..1764)
                     /locus_tag="VF516_37935"
     CDS             complement(934..1764)
                     /locus_tag="VF516_37935"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8113592.1"
                     /translation="MTAERTPVLPPESQGAAPGRGRLTGRRILVVGAGQNDYGETDPP
                     IGNGRAMAVLFAREGARVAVADRDRPSAEATVARIASEGGTAATVIADMAKPGDPQTM
                     VSEASAALGGLDGLVYNVGIADRHGLEGTTSDSWDHVLAVNLRGAMLTARAALPVLGD
                     GASIVFTSSVAGVRPGSRIPSYDASKAALGGLMRHVALEGAARSIRANIVAPGLMDTS
                     IGRAATRGRPSRGQTRIPLGRQGTGWETAYLALFLISHEAAYITGQTFIIDGGLTALA
                     "
     gene            2342..2500
                     /locus_tag="VF516_37940"
     CDS             2342..2500
                     /locus_tag="VF516_37940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113593.1"
                     /translation="MKRTRKQEQERQQRLDGTVRGLVEAELARVVGGDLYLHNPRGSS
                     DGSSGSGG"
     gene            complement(2535..>2856)
                     /locus_tag="VF516_37945"
     CDS             complement(2535..>2856)
                     /locus_tag="VF516_37945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113594.1"
                     /translation="VFADRYHLEVITTPTRARHALGYVLGNWRKHGEDRQGLASTWLV
                     DPFSSGILFPDWKELEDQAWMWPIRETYDPMVVRRPQTWLLREGWKVGGGAISARDVP
                     SQRR"
BASE COUNT          436 a         1035 c          958 g          427 t
ORIGIN      
        1 cacgagatgt tcgggttgtt ggagcagcgc ccgccgcacg atgcgtcggt gaagcagctg
       61 gaccccgtca ccgagcaggt cccgatcagg cacagattcg acgagcaggt ggtgttgccg
      121 ctgcaggtcg cgccgttggc gagcagtggt gtgcaggtgc gcatcccgac cggcgcggcg
      181 gggtcgcaga acaggccctt ctggcactgg ggggtcgagg tgcaggcgac gccagcgccg
      241 agcaacgcgc gacacaccgg gccgccaacc gcggcggtgt cgcagaacaa cccgctggcg
      301 cagccctgca ccgagcacgg catgctctcg gtcggcagcg cgacgcatgt ccggttcgag
      361 gcgcagaagt tatccttcgc gcactcgtag ccgaagtcgc acgcgccgcc ggcctcgacg
      421 atgccggtcc atgcgctggt cacgcacgcc atcgcccacg gcttgaccgc ctcgaccgtc
      481 acgcacgtgt tcgacggcgc cacgaactcc ttcaggcagt cattcatgac cttcgcgtcg
      541 aacttcacgt gcttgttctt gatcgagaag tcgaggtccg cggcctggcg ccggcaccgg
      601 gtggccagat cgctctggca ctcggcctgc gtgcgagggt cgctgaccat cagggtccgc
      661 tcgatctcgc cttccgagca gcattgatac atgtcgaagc aggcgacctc ggcgatctgg
      721 tcgcacacgt tatcttgcgt cgtgttgata tccgggccat tgccgccgca gccgaccagc
      781 acgacgaatg caccacaggc gatcgctgtt cgcatagcac cctccggcgc gaatcctatc
      841 gcggtgatcg ggggactcgc cagcgctgcc gtgagatccg ggtgagcagc tggcaaattc
      901 cggtgagaaa caccgtcccc ggccgccggg ccgtcacgcc agcgcggtca gcccgccgtc
      961 gatgatgaac gtctgccccg tgatgtacgc cgcctcgtgc gagatcagga acagcgcgag
     1021 gtacgcggtc tcccagccgg tgccctggcg gccgagcggg atccgcgtct ggccgcgcga
     1081 cggtcgcccg cgcgtcgccg cgcgcccgat cgacgtgtcc atcagcccgg gcgcgacgat
     1141 gttggcgcgg atcgaccgcg ccgcgccctc gagcgcgacg tggcgcatca gcccgccgag
     1201 cgcggccttc gacgcgtcgt acgacgggat ccggctgccc ggccggacgc cggcgaccga
     1261 cgacgtgaac acgatcgatg cgccatcgcc gagcaccggc agcgccgccc gcgcggtcag
     1321 catcgcgccg cgcaggttga ccgccagcac gtggtcccag ctgtccgagg tcgtgccctc
     1381 gagcccgtgg cggtcggcga tgccgacgtt gtagaccagc ccgtcgagcc cgccgagcgc
     1441 ggcgctggcc tcgctcacca tggtctgcgg atcccccggc ttcgccatgt cggcgatcac
     1501 cgtcgccgcc gtcccgccct cgctcgcgat ccgcgccacc gtcgcctccg cgctcggccg
     1561 gtcgcgatcg gccaccgcga cccgtgcgcc ctcgcgcgcg aacagcaccg ccatcgcccg
     1621 gccgttgccg atcggcgggt cggtctcgcc gtaatcgttc tgtccggccc cgaccaccag
     1681 gatgcggcgg ccggtcagcc gaccgcgccc cggcgcggct ccctgcgact ccggaggcag
     1741 caccggcgtg cgctcggccg tcatcgtccg gctccccgtg caggcagcat cgatatctcg
     1801 caatgggtca tggcgcgctc ccgcagaaag cgcgcatgtt accccggcgc ggtggctggc
     1861 acggggcctt ctgccgcgcc ttgggcacca ccttggtagc cgtccagcgc aatgctagat
     1921 ggcgttcacg accagcgatg ttccggttcg gctgagcgag gccttggggg gggcagacgg
     1981 taaagaagct caaagatgcg tagctacgcc ccagagctcc cccagcgcct cgcgttcgcc
     2041 gtcacggcgg cggggcgagc cgccgcggcc aggttgattc gcgagcgcaa cccggttcgc
     2101 catggcgtca ctgacgatgg ccgttcgtac caggcaacgt cgcaggcgat cgatcctcca
     2161 gccgaagcga tgaaccccca gcgctgatgc cttgccatcc cctcatcggc gtcgatgaca
     2221 tcgtcgatgc cgcgaaatgc cagcggcgtt caccctggct cggcgacaat gcgcggctgc
     2281 ggggagcggg ggcctgcggt catgacgacc ggccgacgcg catcctcaca aggaggcagc
     2341 gatgaagcgc acgagaaagc aagagcagga gcggcaacag cgactcgacg gtacggtgcg
     2401 cgggctggtg gaggccgagc tcgcccgtgt ggttggcggg gacctctacc tgcacaaccc
     2461 ccgcggatcc agcgacgggt cgtcgggctc cggcggctga ccgccgcagc acgtcggagg
     2521 cgcggtcggc ccgctcaccg ccgctggctc ggcacatcgc gcgcgctgat cgcgcctccg
     2581 ccgaccttcc agccctcccg gagcaaccag gtctgtggcc ggcgaaccac catcggatcg
     2641 taggtctcgc ggatcggcca catccaggct tggtcctcca gctccttcca gtccggaaac
     2701 aggatccccg atgaaaaggg atcgacgagc cacgtgctcg cgaggccctg gcggtcttcg
     2761 ccatgctttc gccaattccc gaggacgtac ccaagagcgt ggcgcgcccg cgtcggtgtc
     2821 gtgatcacct cgagatggta gcgatcggca aatacc
//
LOCUS       DASVOR010002046         7674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_226362,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002046 DASVOR010000000
VERSION     DASVOR010002046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7674
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226362"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF516_37950"
     CDS             complement(<1..255)
                     /locus_tag="VF516_37950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113595.1"
                     /translation="MPKHARRLAPPDAVVGETELARWFFAGRYADVIAATYDRGGEIA
                     TADVGFMVGALTFVDRVDDALGAFEVWRSRAGAEPAPRTLA"
     gene            404..736
                     /locus_tag="VF516_37955"
     CDS             404..736
                     /locus_tag="VF516_37955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113596.1"
                     /translation="MVKHAVRYMWFSALAFAAGCSPAHSDSISAVTIEVTCGSTADCP
                     SGFECNADIEHGPPTALCESQDPSATCPSGYETKVMYGQIFCKPPAPVAAGARAGRTS
                     AHRHTAGL"
     gene            complement(741..3800)
                     /locus_tag="VF516_37960"
     CDS             complement(741..3800)
                     /locus_tag="VF516_37960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX8113597.1"
                     /translation="MLAAAWGCSGPPDPGTHITAQPVTTVIFSETFAGGLGQFTAAGN
                     ASVAAGAAHMTGATMASNTGVITSKPFSTVGFSSITLSFDRVTAGLDAGEAGIAEVSI
                     GGGAFAAVESTQVTALGRVTLSLGASAANQAQVVLRFRVSANATGETYDVDNVTIEGV
                     AGATGAPPIGEFVAFETGPVRPLAVSSDGTRLYAVNTPDNRVEVLDVSGATPRPIESI
                     PVGLEPVAVALTGDGQLWVVNQLSDSVSVVDVSASPARVVNTLLVGDEPGDIVFAGPN
                     NAWAFITAAHRGQNAPFDPQLTTPGIGRADVWVFDRTNLGAALGGQPLAILNLFGDTP
                     RGLARSADGTKVYAAVFHSGNRTTVLTSDIASGGLTKPPPSTAADGMTQPRTGLIVQF
                     NGTKWLDSGDPKTGVAPRDWSSHVRLSLPDFDVFAINATGAAPQLGARAAGVGTTLFN
                     LAVNPKSGVVYASNLESRNLTRFEGPGTRSTTVRGHFAESRITVLNGTTVAPRHLNKH
                     ITSYGADLGTATERALSLATPLQMAVTGDGATLFVVGMGSSKLARYSTAQLEADTFTP
                     SLADQLVVSGGGPTGVVLDEAHGRAFVSTRFDNGISVVDTQRFTESAHVTMFDPEPAD
                     VVAGRSFLYDATLSSSRGDSACSGCHIFGDVDHLAWDLGNPDGTTVQNPGTYNVNIPT
                     SLQNRSNHPMKGPMTTQSLRGLARNGPMHWRGDRTGVTHDATETLEDQAFEDFNVAFP
                     SLLGRDSQLTSTQMEAFAKFALRLTYPPNPIANLDNSLTAAQSQGAQVFNTVVSDRLT
                     QCNGCHVLNAPQGRFGTDGTLAFEGGVVAEDFKIPHLRNLYQKVGMFTSNTATGTNIG
                     DQIRGFGYDNEGDIGTVSQFVSLPVFSLTATQRGQVEQFVLAFPSDMNPIVGQQVTVT
                     PASASRADVQGRLNLLVQRAAVTSPRPECELVAKAVLGQAMGWVMNAGQSFVPDATGE
                     AAVSLQGLLNQAAAAGAAVTFTCVPPGNGTRIGIDRDADGIPDRSDS"
     gene            complement(4095..4733)
                     /locus_tag="VF516_37965"
     CDS             complement(4095..4733)
                     /locus_tag="VF516_37965"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HEX8113598.1"
                     /translation="MHVIVVGGGIGGLTLGLLLHARGIGCEIYEAAPAMRELGVGINV
                     LPHAIAELAELGLLARLDEVAVRTRELIYTNRFGQEVWRELRGLDVGYEVPQLSIHRG
                     RLHGVLYRAVIERLGRDAVHTGHRLTGFAQDGRVACAWFDGGRSAAGDAVIGCDGIHS
                     AVRRQLYAGARIDDVIGRAEREAIVNGYARLAGFAPDQVNHVDRPARESRRV"
     gene            complement(5368..6759)
                     /locus_tag="VF516_37970"
     CDS             complement(5368..6759)
                     /locus_tag="VF516_37970"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX8113599.1"
                     /translation="MSGPDWRMVDRALRAIRQRRAALDAEEARWLREAEVLQIWRPLG
                     MVSALDYMERVLGYAPRTAQDRLRVARALGDLPQLTAALAADELAFSAVRELTRVATP
                     GTEAAWIAAAAGKNLRQIEELVADHRPGDRPEDPPDPQARTHVVRFELAAETFARLRQ
                     ARTVLDDEHGTNLSDDGFIAALCSAVLDGAMNTETSGRAKYQIAVTVCQRCRQGWQEG
                     AGAQVAIEPAAIDRAMCDAQHIGSIDSTVPERAYQDIPPSTARLVWRRDGGRCRVPGC
                     RSARGLEIHHVVHRADGGSHDASNLCLLCSACHLAHHRGALTIAGTVDRLEVRRPGQP
                     PQAGDAMPRARSTTRISAEAHEQRCDATLNDTGSVALDANCTATSDSDTGAHVGAPGP
                     ASNAGGLEIKAVSKLDAAILCTQARAALTGLGWKPAIARAAVDTAAAAQGPAMTLERL
                     IFESLRRCPAPRA"
BASE COUNT         1145 a         2773 c         2560 g         1196 t
ORIGIN      
        1 cgccagcgtc cggggcgccg gctccgcccc cgcgcgcgac cgccacacct cgaacgcgcc
       61 cagcgcatcg tcgacccggt cgacgaacgt cagcgcgccg accatgaacc cgacatcggc
      121 ggtcgcgatc tccccgccgc ggtcgtatgt cgccgcgatc acgtcggcgt agcggcccgc
      181 aaagaaccag cgcgccagct cggtctctcc gaccaccgca tccggcgggg caaggcgcct
      241 ggcatgtttc ggcacgacgc aattatatgc ttgtcttcca tgaagaaatt gtgaatcgcg
      301 tcatgaacgc gagttcgaga aagccatcga aactcgatcg ccatgccgga tctcaggtac
      361 ttgcatcgtt gtgcagcgcg tcttccccgg cgcgaggaca gtcatggtca agcacgcggt
      421 acggtacatg tggttctcgg cactggcgtt cgcggccggc tgcagcccgg cgcactccga
      481 ctcgatctcc gcggtgacca tcgaggtcac gtgcggctcg accgccgact gcccgagcgg
      541 cttcgagtgc aacgccgaca tcgagcacgg cccgccgacc gcgctgtgcg agtcacagga
      601 cccgtcggcg acctgtccct ccggctacga gaccaaggtg atgtacggcc agatcttctg
      661 caagccgccg gcgccggtcg ccgccggcgc gcgggccggc cggaccagcg cccaccgcca
      721 caccgccggg ctctgagcgg ctagctatcg ctgcgatcgg ggatgccgtc ggcgtcgcga
      781 tcgatgccga tccgcgtgcc attccccggc ggcacgcagg tgaacgtgac cgcggcgccc
      841 gcggccgccg cctggttcag caggccctgc agcgacaccg cggcctcgcc ggtcgcatcg
      901 ggcacgaagc tctggccggc gttcatcacc cagcccatcg cctggccgag cacggccttg
      961 gcgaccagct cgcactccgg gcgcggcgag gtcaccgccg cgcgctggac cagcaggttg
     1021 agccggccct ggacgtcggc gcggctcgcg ctggccgggg tcaccgtgac ctgctggccg
     1081 acgatcgggt tcatgtccga cgggaacgcg agcacgaact gctcgacctg gccgcgctgg
     1141 gtcgcggtca gcgagaacac cggcaagctc acgaactggc tgaccgtgcc gatgtcgccc
     1201 tcgttgtcgt agccgaaccc gcggatctga tcgccgatgt tcgtgccggt cgccgtgttc
     1261 gaggtgaaca tgccgacctt ctggtacagg ttccgcaggt gggggatctt gaagtcctcg
     1321 gcgaccacgc cgccctcgaa tgccagcgtg ccgtcggtgc cgaaccggcc ctgcggcgcg
     1381 ttgagcacgt ggcagccgtt gcactgcgtg agccggtccg acaccacggt gttgaagacc
     1441 tgcgcgccct gcgactgcgc cgcggtcagg ctgttgtcga ggttggcgat cgggttgggc
     1501 gggtaggtca gccgcagcgc gaacttcgcg aacgcctcca tctgcgtgct ggtcagctgg
     1561 ctgtcgcggc cgagcaggct ggggaacgcg acgttgaagt cctcgaacgc ctggtcctcc
     1621 agcgtctcgg tcgcgtcgtg ggtgacgccg gtgcgatcgc cgcgccagtg catcggcccg
     1681 ttgcgcgcga gcccgcgcag gctctgggtc gtcatcggcc ccttcatcgg gtggttgctg
     1741 cggttctgca gcgacgtcgg gatgttgacg ttgtaggtgc ccggattctg gacggtcgtg
     1801 ccgtcggggt tgccgaggtc ccacgccagg tgatcgacgt cgccgaagat gtggcagccc
     1861 gagcacgccg agtcgccgcg gctcgagctc agcgtcgcgt cgtacaggaa cgaccggccg
     1921 gcgacgacgt ctgcgggctc gggatcgaac atcgtgacgt gcgccgactc ggtgaaccgc
     1981 tgggtgtcga ccaccgagat cccgttgtcg aaccgggtcg agacgaacgc gcggccgtgc
     2041 gcctcgtcga gcacgacccc ggtcggcccg ccgcccgaca cgaccagctg gtcggcgagc
     2101 gacggcgtga acgtgtcggc ctcgagctgc gcggtgctgt agcgcgcgag cttgctcgag
     2161 cccatgccga cgacgaacag cgtggcgccg tcgccggtca ccgccatctg cagcggcgtc
     2221 gcgagcgaca gcgcccgctc ggtggccgtg ccgaggtcgg cgccgtagct cgtgatgtgc
     2281 ttgttgaggt ggcgcggcgc gacggtcgtg ccgttgagca ccgtgatccg gctctccgcg
     2341 aagtggccgc gcaccgtcgt cgaccgcgtc cccggcccct cgaaccgggt caggttccgc
     2401 gactcgaggt tgctggcgta gaccacgccg ctcttcgggt tgaccgccag gttgaacagc
     2461 gtcgtgccga cgcccgccgc ccgtgccccg agctggggcg ccgccccggt cgcgttgatc
     2521 gcgaacacgt cgaagtcggg caggctgagc cggacgtgcg agctccagtc gcgcggcgcc
     2581 acgccggtct tcggatcgcc gctgtcgagc cacttcgtgc cattgaattg cacgatcagg
     2641 ccggtgcgcg gctgggtcat gccgtcggcc gcggtcgacg gcggcggctt ggtcagcccg
     2701 cccgacgcga tgtcgctggt cagcaccgtc gtgcggttgc cggagtggaa caccgcggcg
     2761 tacaccttcg tgccgtcggc gctgcgcgcc agcccgcgcg gcgtgtcgcc gaacaggttg
     2821 aggatcgcca gcggctggcc gccgagcgcg gcgccgaggt tggtgcgatc gaacacccag
     2881 acgtcggcgc ggccgatccc cggcgtcgtc agctgcggat cgaacggcgc gttctggccg
     2941 cggtgcgccg cggtgatgaa cgcccacgcg ttgttcggcc cggcgaacac gatgtcgccc
     3001 ggctcgtcgc cgaccagcag cgtgttgacg acccgcgccg ggctcgccga cacgtcgacc
     3061 acgctgacgc tgtcggacag ctggttcacc acccacagct ggccgtcgcc ggtcagcgcg
     3121 accgcgaccg gctcgagccc gaccgggatc gactcgatcg gccgcggcgt cgcgccgctg
     3181 acgtcgagca cctcgacccg gttgtcgggc gtgttgaccg cgtacagccg cgtcccgtcg
     3241 ctcgacaccg cgagcggccg gaccggcccg gtctcgaacg ccacgaactc gccgatcggc
     3301 ggggcgcccg tcgcgcccgc gacgccttcg atcgtcacgt tgtcgacgtc ataggtctcg
     3361 ccggtcgcgt tcgcgctgac ccggaaccgc agcacgacct gggcctggtt cgccgcgctc
     3421 gcgccgagcg acagcgtcac gcggccgagc gccgtcacct gggtcgactc gacggcagcg
     3481 aacgcgccac cgccgatcga cacctcggcg atgcccgcct ccccggcgtc gagccccgcc
     3541 gtcacgcgat cgaagctgag cgtgatgctc gagaacccga ccgtgctgaa cggcttcgac
     3601 gtgatcacgc cggtgttcga cgccatggtg gcgccggtca tgtgcgcggc gccggcggcg
     3661 accgacgcat tgccggccgc cgtgaactgc cccagaccgc cggcgaaggt ctcggagaag
     3721 atcaccgtgg tgaccggctg ggcggtgatg tgcgtgcccg ggtcgggcgg cccgctgcag
     3781 ccccacgctg cagccagcac gcccacgata cttgacctga tccgcatgca agtactcctg
     3841 tcgctgcgtc tgggtggacg gagatcgccc ggtgccggcg ctgccatgcg ccgcgtgctc
     3901 gacgatgctc ccagcgctcc gttgtacagg gccgcgcgac gcgctcgcaa tcgcgccgcg
     3961 gccgtttgat ctgcacatcc tgcgaatacg gatctcacac accgcgacag gacatcggac
     4021 ttaccaattt tcgaagactt gttgtcttgg atcacgcgtg cggtgccgca gcatgcggcg
     4081 ccgggatcga ttcctcacac tcgccgcgac tcccgcgccg gccgatctac gtggttcacc
     4141 tgatccggtg cgaaccccgc gagccgcgcg tagccgttca cgatcgcctc gcgctcggcg
     4201 cggccgatca cgtcgtcgat ccgcgcgccc gcgtacagct ggcgccggac cgccgagtgg
     4261 atgccatcgc agccgatcac cgcatcgccc gctgccgacc ggccgccgtc gaaccacgcg
     4321 caggcgacgc gcccatcctg tgcgaacccg gtcagccggt gcccggtgtg taccgcatcc
     4381 cgtcccagcc gctcgatcac cgcccggtac agcaccccgt gcagccggcc gcggtggatc
     4441 gacagctgcg gcacctcgta ccccacgtcg agcccgcgca gctcgcgcca gacctcctgg
     4501 ccgaaccggt tggtgtagat cagctcgcgg gtccgcaccg caacctcgtc gagccgcgcc
     4561 agcagcccga gctcggccag ctcggcgatc gcgtgcggca gcacgttgat cccgaccccg
     4621 agctcgcgca tcgccggcgc cgcctcgtag atctcgcaac cgatcccccg cgcatgcagc
     4681 agtagcccga gcgtcagccc accgattccc ccgcccacca cgatgacgtg catggtggat
     4741 gagatgcgcg agcccagccg cccgttctgc cgtgagccct ggcccatcgg gcgggatcgg
     4801 gaacgacagt tgtgcatcgg cgcgaggcgc cacgaacctg ctggcgcaat ggaccgtacg
     4861 actcggttcc gcgcgcccac gtgggcgttg cacatgggag cgtgctcgcc agcggcgcgc
     4921 cggctcgaaa cctggttggg gagagcgccc agctggcctg tgaccggcgc tcggtccgtg
     4981 acctgcgcgc agcggggcac cccgttggca tccgcgcgca gctcaccgcc ctcgtcccgc
     5041 cgctcacagt ccccgacgct cgagcccagg cacccttgcc ggaatttgaa tttctcggga
     5101 gaggatgttc aggtccacga agcaaggtgc acgggtaggc acggtctgct gcgtgtcagt
     5161 ccgcgggcac cgccaccagc gcgaacctgc tggcgcaatg gatcctgcgg ctcgggcggt
     5221 acttgcgcgc ctggggccga gtggattttg catgcattac ctgcgaaatc gccaaacgat
     5281 cttgaggatt ccgcgagcat cgcctgcgaa atcggccctc gaagcggccc cccacgtggg
     5341 cgccacgtgg ctcggattca gcggcgctca cgccctcggc gcagggcatc gccgtagcga
     5401 ttcgaagatc agccgctcca gggtcatcgc cggtccctgc gctgccgctg cagtatccac
     5461 cgccgcgcgc gcgatcgccg gcttccatcc cagccccgtc agtgccgccc gcgcctgggt
     5521 gcagagaatt gcggcgtcga gcttgctcac tgccttgatc tcgaggccgc ctgcattcga
     5581 tgccgggccc ggggcgccca cgtgggcgcc ggtgtccgag tccgacgtgg ccgtgcagtt
     5641 cgcatcgagc gcgacgctgc cggtgtcgtt cagcgtcgcg tcacagcgtt gctcgtgcgc
     5701 ctccgcgctg atccgcgtcg tcgacctcgc acggggcatc gcatcgccgg cctgcggtgg
     5761 ttgccccggg cggcgaacct ccagccgatc taccgtgccc gcgatcgtca gcgcaccgcg
     5821 atggtgagcg aggtggcacg ccgagcacag caagcaaaga ttcgaagcat catggcttcc
     5881 gccgtcggct cgatggacca catgatggat ctccaatcct ctcgccgacc ggcagcctgg
     5941 cacgcggcag cgacctccgt cgcgacgcca taccaggcgc gcggtgctcg gtggaatgtc
     6001 ctgatacgca cgctcgggca ccgtgctgtc gatcgacccg atatgctggg cgtcgcacat
     6061 cgcacggtcg atcgccgcag gttcgatcgc gacctgcgcg ccggcacctt cctgccagcc
     6121 ctgccggcag cgctgacaca cggtcaccgc gatctggtac ttcgcccggc ccgaggtctc
     6181 cgtgttcatc gcgccatcga gaaccgcact gcacagcgcc gcgatgaacc catcgtcgga
     6241 caggttcgtt ccgtgctcgt cgtcgagcac cgtccgcgcc tggcgcagcc gtgcgaacgt
     6301 ctccgccgcc agctcgaacc ggacgacatg cgtgcgggcc tggggatccg gcggatcctc
     6361 gggccggtcg ccaggccgat ggtcggccac gagctcctcg atctgccgca ggttcttgcc
     6421 ggcggccgcc gcgatccacg ctgcctcggt cccgggcgtc gccacgcggg tcagctcgcg
     6481 cactgccgag aacgccagct cgtcggccgc aagcgccgcc gtcagctgtg gcaagtcgcc
     6541 gagcgcgcgc gcaacacgca gccggtcctg cgccgtgcga ggggcgtagc cgagcacacg
     6601 ctccatgtag tccagcgccg agaccatgcc gagcggacgc cagatctgca gtacctcggc
     6661 ctcgcgtagc cagcgcgcct cctcggcatc gagcgcggcg cgccgctgcc ggatcgcccg
     6721 cagcgcgcga tccaccattc gccagtccgg cccggacacg gaccgcagct tcgatcccca
     6781 gctcgctact ggcccaatct cgatctctgc ttcgcttgcg gcgtctcgta gcatgacctg
     6841 ctcctgtacg atttcgcgca tcgcttctag cacatgagtt tccgaggcat tgatttgcgc
     6901 ggagtgcgct gatcaacgtt attctgcgaa aatcgcgggt gcagcgcgat ttcgctcgca
     6961 gagcgcaaac cagcgcgtct cggagcggat ccatgcgcga ttcgcaggag ctcgaacaag
     7021 tcccgtcgaa atactgtcaa gcaaaatcga catgcacatg gcgatttcga tcattgactc
     7081 ataaatgcga cctcgattcg caaggcatgc aacgatcgac gacgtggcag tcgccatcca
     7141 gatgcccttg cgagtacggg gaagtgacgt cattcaggtt cgcgtctcac acgacgatcg
     7201 gatgcactca agcgctgcgg agtacgagcg ttcacccgag cggcacttcc agcacagact
     7261 tcatgacact ccgaatgccg ccgagatgat cgaagcgtcg caggttccaa ggtgtcgaat
     7321 gacagtatca agatagggtg gcaaacaccg gcacccacgt ggacgcgcac ggccgggcgg
     7381 catgaccacc gatgaggaag tttcgcgatc ggccttgcct gagcatcctc tccccgagaa
     7441 attcaaagtt cccgaacggt gcccgggctc gagcgtcggg actgtgagcg gcgggacgag
     7501 ggcggtgagc tgcgcgcgga tgccgacgag gtcccccacc gcgcgcaggt cacggaccga
     7561 gcgccggtca caggccagcc gggagatctc ccgaaccagg tttcgagcgg gcgcgctgct
     7621 gacgagcacc ccatgcgcaa cgcccacgtg ggcgcgcgcg gaaccgagcg cagg
//
LOCUS       DASVOR010002047         4794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_167523,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002047 DASVOR010000000
VERSION     DASVOR010002047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167523"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..884
                     /locus_tag="VF516_37975"
     CDS             <1..884
                     /locus_tag="VF516_37975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEX8113600.1"
                     /translation="EASTGGAPSIAVKDPALQRVAEAELDHAMTAHHATGAIAIVLDA
                     RTGAVLAAATRGDGDARAARSPGSTMKPFTFAAVLEAGIVDAGARIDCENGTRRYGDR
                     TLADASPHGTLDLGGVLAVSSNVCTAKLAEPLGDRLAESLRRYHFAAPAHIDTRSLEG
                     ASIALGEGIRASALDVAAGYTAFADGGVYHAPDGNSERVMRSDTARTVMTMLDRVVND
                     ADGTGQAARIDGIRVAGKTGTAQSRGGRHYASFIGIVPADAPRFVVLVGVDGVTGAGG
                     KVAAPVFAKIASRALGR"
     gene            complement(906..1940)
                     /locus_tag="VF516_37980"
     CDS             complement(906..1940)
                     /locus_tag="VF516_37980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113601.1"
                     /translation="MAYILDSNDGRRTGRLRVYDLTTRRELHSMQTGYCPDFAISPDG
                     AWLFLAQTDLEQTPCVHEVRCLEAASLRECYRVALLDRALYNIAPSLGGVAVTPDGRH
                     CLVLKTRALADDLAEFSIAILDTQRQCWLEHELALPHMVMGNGPLGSGPDFHFALSSS
                     GGEPVGIGDPVRAEKLRDVLPSPFPEAMRRPGLLRAAGAHPRGGRVHYVSHEGMLVTW
                     DVATGALSVPAPLLLPPGMSVRSQHILVDGSQMYLGVAGPEWDGRGHAEMVCGFSQDS
                     WERKLALELEAPCSKLALDRNGQLLFGLSFATRSLLAVDLRAQATLFEITDVGRTPAL
                     FAVLGGPAGG"
     gene            complement(1967..2548)
                     /locus_tag="VF516_37985"
     CDS             complement(1967..2548)
                     /locus_tag="VF516_37985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113602.1"
                     /translation="MMDFITPVVHRDRAGWRIALIAYGACAALSAAGFGALLGMGGRA
                     LRQLPIEGGAAALPVIIFLAVLHELGVVRIPWPQRRWQVPSSWRFIEPRWIPSALYGL
                     LLGPGILTYIPVATYYVLVTMAILITDPGRAAAVFALFAVAQLVPLYLVSRGADTFDA
                     AAARIRTWSGHRVLLHRLNGALLAAVAVHLVCH"
     gene            complement(3150..3722)
                     /locus_tag="VF516_37990"
     CDS             complement(3150..3722)
                     /locus_tag="VF516_37990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113603.1"
                     /translation="MTGIWLASYVILWGVVIALGVLLLTTLRLVGVLHHRLGPTGALV
                     VDEGPRLDESLATEISRTVPALRFPRERDVLIVFLSETCPSCLNLMDSLVAFSRRNAG
                     AVDVLLVPTEAGFREDGPIASRAAGLRLVSDPSLARAWRIAVTPFGLWIDRAGMARGK
                     GIVNHIEHLDSLLHTARRSDRSAGPSMVTS"
     gene            complement(3719..4375)
                     /locus_tag="VF516_37995"
     CDS             complement(3719..4375)
                     /locus_tag="VF516_37995"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HEX8113604.1"
                     /translation="MNVLTLNLWMDKTYCDDQMLTGADGVCPLAATWLLASVLLHAGL
                     AKAGRPREFASALRDEYRVAIRPLQIAILLSWSEIALAILLVITPSRDWVVWATVLLL
                     GIFTAVLLRVRWQGAVTIACSCFGMEEARWPIRWLLLRNLALVVLAVYLLLPQNAAIV
                     SAPRPAVLPGLLSLLGLAMGAMFARAAHRAWRTRQQALEGIRLLVQAGAERSFRWSLQ
                     "
     gene            complement(4531..>4794)
                     /locus_tag="VF516_38000"
     CDS             complement(4531..>4794)
                     /locus_tag="VF516_38000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113605.1"
                     /translation="WPAHGYQTEAVYNLHARQPVAAMRGAPWSGDPPILAGRSAVFRR
                     EPLRWSTWTAMWEQLARGEPPAPVVLGPSLIRRPPPSATQLAG"
BASE COUNT          788 a         1651 c         1745 g          610 t
ORIGIN      
        1 gcgaggcctc caccggtggc gcgccgtcga tcgcggtgaa ggatccggcg ctccagcggg
       61 tcgccgaggc cgagctcgac cacgcgatga ccgcgcacca cgcgaccggc gcgatcgcga
      121 tcgtgctcga cgcgaggacc ggcgccgtgc tcgcggccgc gacccgcggc gatggcgacg
      181 cgcgcgcggc ccgctccccg ggttcgacga tgaagccgtt cacgttcgcg gcggtgctcg
      241 aggcggggat cgtggacgcc ggtgcgcgca tcgactgcga gaacggcacg cgaagatacg
      301 gcgacaggac gctcgccgac gcgtcgccgc acggcacgct cgacctcggc ggcgtcctcg
      361 cggtgtcgtc gaatgtgtgc accgcgaagc tcgccgagcc gctcggcgat cggctcgccg
      421 agtcgctgcg tcgctatcac tttgcggccc ccgcgcacat cgacacgcgc tcgctcgagg
      481 gcgcgtcgat cgcgttgggg gaaggcatcc gcgcctcggc gctcgacgtc gccgccggct
      541 acaccgcgtt cgcggacggc ggcgtctatc atgcgcccga cggcaacagc gagcgcgtga
      601 tgcggtccga taccgcgcgc accgtgatga cgatgctcga tcgcgtcgtc aacgacgccg
      661 acgggaccgg ccaggcggcg cgcatcgacg gcatccgtgt cgcgggcaag accggcaccg
      721 cgcagtcgcg cggtggccgc cactacgcga gcttcatcgg catcgtcccg gccgacgcgc
      781 cgcggttcgt cgtgctcgtc ggcgtcgacg gcgtgaccgg cgcgggcggc aaggtcgccg
      841 cgccggtgtt tgcgaagatc gcgtcgcggg cgctggggcg gtaggggagg gattgccggg
      901 tggcttcagc cgccggcagg gccgccgagc accgcgaaca gcgccggcgt ccgccccaca
      961 tccgtgatct cgaacagggt ggcctgcgcg cggagatcca ccgcgaggag cgaccgggtg
     1021 gcgaagctga ggccgaacag cagctgtccg ttccggtcga gcgcgagctt ggagcacggg
     1081 gcttcgagct cgagcgcgag cttgcgctcc cagctgtcct gggagaagcc acagaccatc
     1141 tcggcgtggc cgcgtccatc ccactcgggg ccggcgacgc cgaggtacat ctgcgatccg
     1201 tcgacgagga tgtgctggct gcgcaccgac atgcccggag gcagcagcaa gggcgcgggg
     1261 acggacagcg cgcccgtcgc gacgtcccag gtcaccagca tcccctcgtg agagacgtag
     1321 tggacccgcc ctccgcgcgg atgcgcgccc gcggcgcgga ggaggccggg gcggcgcatc
     1381 gcctcgggga acgggctggg gaggacgtcc cgcagcttct ccgcgcggac cgggtcgccg
     1441 atcccgacgg gctctccgcc gctgctggac agcgcgaagt ggaagtcggg ccccgacccg
     1501 agcggcccgt tccccatcac catgtgcggc agcgcgagct cgtgctcgag ccagcactga
     1561 cgctgggtat ccaggatggc gatggagaac tccgccagat catccgccag cgccctggtc
     1621 ttgaggacca ggcagtgccg gccgtccggg gtcacggcga cgccgccgag gctgggggcg
     1681 atgttgtaca gggcgcggtc gagcagcgcg acccggtagc attcccggag cgaggcggct
     1741 tccaggcagc ggacctcgtg gacgcacgga gtctgttcga ggtcggtctg cgccaggaac
     1801 agccaggctc cgtccggtga gatcgcgaag tccgggcagt aaccggtctg catgctgtgc
     1861 agctcccgcc gggtggtcag atcgtagacg cggagccgtc cggtccggcg gccgtcgttg
     1921 gagtcgagga tgtaggccaa ccacattggg ttcctccgga tcgcagtcag tgacagacca
     1981 gatgcaccgc gacggccgcc aggagcgcgc cgttgagccg gtgcaacagc acccgatgac
     2041 cgctccaggt ccggatccgc gccgccgccg catcgaaggt atcggcgcct cggctcacca
     2101 ggtagagcgg cacgagctgt gcgacggcga acagcgcgaa caccgccgcc gcacgccctg
     2161 gatccgtgat gaggattgcc atcgtcacca ggacataata ggtggcgact ggaatgtacg
     2221 tcaggattcc cggcccgagc agcagcccgt acaaggcact cgggatccag cgcggctcga
     2281 tgaaccgcca ggacgacggc acctgccatc tgcgctgcgg ccagggaatg cgcacgacgc
     2341 cgagctcatg gagcacggcg aggaagatga tgaccggaag cgcggcggcc ccgccttcga
     2401 tcgggagctg gcgcagcgcg cgccccccca tccccagcag cgccccgaaa cccgccgccg
     2461 agagcgcggc acaggccccg tacgcgatca aggcgatcct ccacccggcg cggtccctgt
     2521 gcacgacagg ggtgatgaag tccatcatcg agagcattca aggcgaccac gccgtcgagt
     2581 agccggccag cgcggcgcac agcagggccg ggccgatgag ctcggagctc aacatggccc
     2641 gaggtcttcc gagagggtgc agacgtactg catgttgccc gcgccggcac accagttcgg
     2701 ctgactggag ttgtcgcacc agacgcagtc cgccgggcag gtcacattgc cgcagcagtc
     2761 ccggaacgag cgggtgcgac caccgcagca ataggtccag ctcgagccgc gggaggtccc
     2821 tgcgggacag gcggtgtccg ttccaccgct gcagctgcag gggtggccgt gcatccagca
     2881 gtagcgatct tcgctgcacc ggggaggcgc taccggctcc ggatcgggcg cgggagagct
     2941 cggctgcagg agcgtcccca gcatgcgcga cagcctgcgc aacctctcgg gccagaccgc
     3001 acgcggccgg tgcccctcgc ggagggcaag gggcggggta taggagtagc ctgccgccgc
     3061 gtaccccagg cgacccacga gcgtggcgca ggaaaaccgc agcgaaagcc agcgcgcgca
     3121 ggcgatcagg atgcgttcga tccagtgagt catgacgtca ccatcgaggg gcccgccgat
     3181 cggtccgatc gccgggcggt gtgaagcagg ctgtccaggt gttcgatgtg gttgacgatg
     3241 cccttgccgc gcgccatccc cgcccgatcg atccacagcc cgaacggcgt gacggcgatg
     3301 cgccacgccc tggcgagcga cggatccgac accagccgga gcccggccgc gcggctcgcg
     3361 atcggcccgt cctcgcggaa gccggcttcg gtcgggacga gcagcacgtc gacggctccg
     3421 gcattccgcc gcgagaaggc gaccaggctg tccatcaggt tgaggcagct cggacacgtc
     3481 tcggacagga agacgatcaa cacgtcgcgc tcccgcggaa agcgaagcgc tggaacggtc
     3541 ctcgagatct ccgtggccag cgactcgtcg agccgggggc cctcgtccac gaccagggcg
     3601 ccggtgggcc ccaggcgatg gtggaggacg ccgaccaggc gcagcgtcgt gagcagcagt
     3661 acgcccaggg cgatgacgac gccccacagg atgacatagg aggccagcca gatccccgtc
     3721 attgcagact ccatcggaac gagcgctctg cgcccgcctg cacgaggagg cggatcccct
     3781 cgagcgcttg ctgccgcgtc cgccacgccc gatgggcggc gcgggcgaac atggcaccca
     3841 tggcgagccc caggagcgac agcaggccag gcaggaccgc gggccgaggc gccgagacga
     3901 tcgcggcgtt ctgcggtagc agcaggtaca cggcgaggac aaccagggcc aggttccgca
     3961 ggagcagcca gcggatcggc cacctcgctt cctccatgcc gaagcaggag cacgcgatgg
     4021 tcaccgcgcc ctgccagcgc actctcagca ggacggcggt gaagatgccg agcagcagca
     4081 cggtcgccca tacaacccag tcgcgcgacg gcgtgatgac gaggaggatc gccagggcga
     4141 tctcgctcca ggacaggagg atcgcgatct ggagcggacg gatcgcgacg cgatactcgt
     4201 cgcgcagcgc ggaggcgaac tcccggggcc gcccggcctt cgccaggccc gcgtggagca
     4261 gcacgctggc cagaagccag gtcgcagcga gcgggcacac gccgtctgcg ccagtcaaca
     4321 tctgatcgtc gcaatatgtc ttatccatcc agaggttcaa ggtgagcaca ttcatgctga
     4381 ttcggactgc tggatatgtt tgcaagacaa tgccggtatc gtgcagagat tgccatctgg
     4441 caggactctc gcgctgttca tggcgggctg cgccacgcaa gccgtgcgtc ggcgctcggt
     4501 cgccgcggac gtgctcgtcc ccggtggaga tcacccggcg agctgggtgg cgctgggcgg
     4561 cgggcggcgg atcaggctcg ggccgaggac gaccggggca gggggctcgc cgcgcgcgag
     4621 ctgctcccac atcgcggtcc aggtcgacca gcgcagcggc tcgcggcgga acaccgcgct
     4681 gcggccggcg aggatcggcg gatcgccgga ccacggagcg ccgcgcatcg cggcgaccgg
     4741 ctggcgcgcg tggaggttgt agaccgcctc ggtctggtag ccgtgggcgg gcca
//
LOCUS       DASVOR010002048         1806 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_518053,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002048 DASVOR010000000
VERSION     DASVOR010002048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1806
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518053"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VF516_38005"
     CDS             complement(<1..297)
                     /locus_tag="VF516_38005"
                     /inference="COORDINATES: protein motif:HMM:NF018938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEX8113606.1"
                     /translation="MNRTIRIGCGSGFWGDSALGAAQLVERGEIDVLVLDYLAEITMS
                     ILARMRARRPDAGYATDFVDAVIAPLAPQLAARRIKVVANAGGVNPAACQARVAQ"
     gene            complement(294..1067)
                     /locus_tag="VF516_38010"
     CDS             complement(294..1067)
                     /locus_tag="VF516_38010"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8113607.1"
                     /translation="MITTESRGVARVVTIDRPEQGNALNEAVASGIIAALDAAEADPA
                     CRVVVLTGRGDKVFCAGGDLKSTGGDAPFDVDPSDPRNFIVTLLRRMAECRLPLLARV
                     NGHAVAGGMGLVCACDLAIASDRARFGVPEVGIGTFPMMILPFLMRVLPARTLLELCL
                     TGDLIDAADARACGLVNRVVPPAELDAATDALVARLASRSPTAVRLGKQGVHAMRDMA
                     LGEALEYAQLMIGLMVRTRDAAEGIAAFRAKRPPEWTGR"
     gene            complement(1064..>1806)
                     /locus_tag="VF516_38015"
     CDS             complement(1064..>1806)
                     /locus_tag="VF516_38015"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8113608.1"
                     /translation="ARVVLAGRTPARLEALAGELAAFPVAVHAADVRERDAVDALFAA
                     HPCDLVIHAAGGQYPQPAIELSAKGFRAVIATNLEGAFHVMQAAARAWRDAGRGGSLV
                     NVVVSPRGLHHVSHTVAARAGVKAFSEAVAVEWAPLGIRVNCVAPGAIETAGWAAYAP
                     EVAARYPNSNPLRRAGTAWDVATACIFLAGPTGGFITGETLEVSGGGHLWGETWTTAK
                     PAWFRAASRALDPDLGSDLGSALEEAAR"
BASE COUNT          235 a          687 c          669 g          215 t
ORIGIN      
        1 ctgcgcgacg cgcgcctggc acgccgccgg gttcacgccg cccgcgttgg ccacgacctt
       61 gatccggcgc gccgccagct ggggggcgag cggcgcgatc acggcgtcga cgaagtcggt
      121 cgcgtagccg gcgtcgggcc ggcgggcgcg catccgcgcg aggatcgaca tcgtgatctc
      181 ggcgaggtag tcgagcacca gcacgtcgat ctcgccgcgc tcgaccagct gggccgcgcc
      241 gagcgccgag tcgccccaga acccgctgcc gcagccgatc ctgatcgtcc ggttcatcgt
      301 ccggtccact ccggcggccg cttggcgcgg aacgccgcga tgccctccgc cgcgtcccgg
      361 gtccgcacca tgagcccgat catcagctgg gcgtactcga gcgcctcgcc gagcgccatg
      421 tcgcgcatcg cgtgcacccc ctgcttgccg agccgcaccg ccgtcggcga ccggctcgcg
      481 agcctggcga ccagcgcgtc ggtcgcggcg tcgagctcgg cgggcggcac cacgcggttg
      541 accagcccgc acgcgcgtgc gtcggcggcg tcgatcagat cgccggtcag gcacagctcg
      601 agcagcgtgc gcgccggaag cacgcgcatg aggaacggca ggatcatcat cgggaacgtg
      661 ccgatgccga cctcggggac gccgaaccgc gcgcgatcgc tggcgatcgc gagatcgcac
      721 gcgcacacca ggcccatccc gccggcgacc gcgtggccgt tgacccgggc caggagcggc
      781 agccggcact ccgccatccg gcgcagcagc gtgacgatga agttgcgcgg atcgctcgga
      841 tcgacgtcga acggcgcgtc gccgccggtc gacttgagat cgccgccggc gcagaacacc
      901 ttgtcgccgc gcccggtgag cacgacgacg cggcacgccg gatcggcctc ggccgcgtcg
      961 agcgcggcga tgatgccgct cgcgaccgcc tcgttgagcg cgttgccctg ctcggggcga
     1021 tcgatcgtga ccacgcgcgc gacgccgcgc gactcggtgg tgatcatcgg gctgcctcct
     1081 cgagagccga gccgaggtcc gagccgaggt ctggatcgag cgcgcgcgac gccgcgcgga
     1141 accacgccgg cttcgccgtg gtccaggtct cgccccacag gtggccaccg ccgctgacct
     1201 cgagcgtctc gccggtgatg aagcccccgg tcggacccgc caggaagatg cacgcggtcg
     1261 cgacgtccca cgccgtgccg gcgcggcgca gcgggttact gttgggatag cgcgcggcga
     1321 cctcgggcgc gtacgccgcc cagccggcgg tctcgatcgc gccgggcgcg acgcagttga
     1381 cgcggatgcc gagcggcgcc cactcgacgg cgaccgcctc gctgaacgcc ttgacgccgg
     1441 cgcgcgccgc gaccgtgtgg ctgacgtggt gcaggccgcg cggcgacacc acgacgttga
     1501 ccaggctgcc tccgcgcccg gcgtcgcgcc acgcccgggc ggcggcctgc atgacgtgga
     1561 acgcgccctc gaggttggtc gcgatcacgg cgcggaagcc cttcgcgctg agctcgatcg
     1621 cgggctgcgg gtactggccg ccggccgcgt ggatcaccag gtcgcacggg tgcgcggcga
     1681 acagcgcgtc gaccgcgtcg cgctcgcgca cgtcggcggc gtggaccgcg accgggaacg
     1741 ccgcgagctc gccggcgagg gcctcgagcc gggccggggt gcgcccggcg agcacgacgc
     1801 gcgcac
//
LOCUS       DASVOR010002049         2606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_110295,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002049 DASVOR010000000
VERSION     DASVOR010002049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110295"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VF516_38020"
     CDS             <1..470
                     /locus_tag="VF516_38020"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8113609.1"
                     /translation="AVAKAEPASTTTTAPAAPAAPVATAAPTPTAPTPTAPTPTAAPA
                     ASPGPTPTRPVAIAPSALELQRVAGDTDVHPSKQTRSAMIRDGVSSVKGTVRVCVDTV
                     GVVTETMLTEATGYDEYDKKLIAAVRGWRFRPYVVNGQTFAVCSTAEFVYVPR"
     gene            798..>2606
                     /locus_tag="VF516_38025"
     CDS             798..>2606
                     /locus_tag="VF516_38025"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8113610.1"
                     /translation="MTGAVAKGPAVFDWSARQRADLDALWRDPDSEGARERLAKDLAA
                     FCEKLDWAADAQKLEDAGKRGGEYTLTLSAVPPELYVLPWEVIRVGESGTYLADHPST
                     QVRYAIPGLPAREIQTAPPRPGVLFAWSGAGGEVPHEEQAAAIRGAAADGGVAFHEVA
                     HVDAASLQAALDAGPPSVLHLLCHGVAGRDGEPSGLRIGTLDQPSEISAQRLARMLRH
                     HEGALRLVVLSACGSGDGRGDPLLLGSLAQELHRNGIRNVVASRYPLSVPGSQVMTKA
                     LYGKMLGEAWSLERALRHARQTLLRVGADGEAHPGDAYGIQLYAHDTERFVSDNDLAA
                     ERPVLASYPFGTAACPVPAKGPPRVELTLEIDARPSMSDKALTRRLRELSEDDHLTAA
                     IEPDAEAGPPKLKVHTTVDGSQRLLRSWRSKALPVAIGVLVLDLTKTANASPLPPVKP
                     VVAHRLAAKLGKATSGAAKLVSAKVAITLISVTAVVAAAAALHPQASTGGNGPQGRAK
                     QYGDGHTIEGGGSSATRQIEPPPVCGNGKVERDEQCDDGNRVDGDGCSAACMTEPPPV
                     CGNGKVERGEACDDGNRVDGDGCSAACMTEPPPVCGN"
BASE COUNT          390 a          875 c         1012 g          329 t
ORIGIN      
        1 ccgcggtcgc gaaggccgaa ccggcctcga ccaccaccac cgcccccgcc gcgcctgcag
       61 cccccgttgc gacagcagcc ccgacgccga cggccccgac gccgacggcc ccgaccccga
      121 ccgcagctcc cgcggcgagc ccgggcccga ccccgaccag gccggtcgcg atcgcgccga
      181 gcgcgctgga gctccagcgg gtcgccggcg acaccgacgt gcaccccagc aagcagacga
      241 ggtccgcgat gatccgggac ggcgtgagca gcgtgaaggg cacggtgcgg gtgtgcgtcg
      301 acacggtagg cgtcgtgacc gagacgatgc tgaccgaggc gaccggctac gacgagtacg
      361 acaagaagct gatcgccgcg gtccgcggct ggcggttccg gccgtacgtt gtcaacggcc
      421 agacgttcgc ggtgtgcagc accgccgagt tcgtgtacgt gcccaggtag cgcggcgtcc
      481 ctcgactcgg cgctcgtgtg acgaggcccg gcgtggcggc cggccacagg tgcgggcccg
      541 tcgcgtcgac tcacatgcgc ccatgagtcg cgcggtgtcg gctgccgcac gaccgtgagg
      601 aagtcgtccg gacgcgatcc attttcctcg gatctccacc tcagatccgt ggtgtccggg
      661 cacggccatc acgtccgccc gggtggggcg gagcgagcgc tggagatggc gatatgttcg
      721 acaagctgca tctgttgagt caggacaatc cggcgggagg cgatgggctg cggtggcgcg
      781 cgcatccgta ctttcgcatg accggtgcgg tggccaaggg gccggccgtg tttgactgga
      841 gcgctcgcca gcgcgccgat ctcgatgcgc tgtggcgaga tccggatagc gagggagcgc
      901 gcgagcggct cgccaaggac ctggcggcgt tctgcgagaa gctggactgg gcggcggacg
      961 cgcagaagct ggaagacgcc ggcaagcggg gcggcgagta cacgctcacg ctcagcgcgg
     1021 tgccgcccga gctgtacgtc ctgccgtggg aggtgatccg ggtcggcgag tccggcacgt
     1081 acctggcgga tcatccgagc acgcaggttc gctatgcgat cccggggctg ccggcgcgcg
     1141 agatccagac cgcgccgccg agaccgggcg tgctgttcgc gtggtccggc gcaggcggcg
     1201 aggtgccgca cgaggagcag gcggcggcga tccggggggc ggccgcggac ggtggcgtgg
     1261 cgttccacga ggtggcccac gtcgacgcag ccagcctgca ggcggcgctc gacgcggggc
     1321 cgccctcggt gcttcacctg ctgtgccacg gcgtggcggg gcgcgatggc gagccgtccg
     1381 gactgaggat cgggacgctc gaccagccgt cggagatctc cgcgcagcgg ctggcgagga
     1441 tgctgcgcca ccacgagggc gcgctgcgac tcgtggtgct gagcgcgtgc ggcagcggag
     1501 atggccgcgg tgatccgttg ctgctgggca gcctcgcgca ggagctgcac aggaacggga
     1561 tccgtaacgt ggtggcgtcg cgctacccgc tgagcgtgcc gggctcgcag gtgatgacga
     1621 aggcgctgta cggcaagatg ctcggcgagg cgtggtcgct ggagcgcgcg ctgcggcacg
     1681 cgcgtcagac gctgctgcgc gtcggcgccg acggtgaggc gcatcccggc gatgcctacg
     1741 ggatccagct ctacgcgcac gacaccgagc gattcgtctc ggacaacgat ctcgcagcgg
     1801 agcgcccggt gctggcgagc tatccattcg gcaccgccgc ttgtccggtg ccggccaagg
     1861 gcccgccgcg cgtggagctg acgctggaga tcgacgcccg gccgtcgatg tccgacaagg
     1921 cgctgacccg ccggctgcgg gagctcagcg aggatgatca cctcaccgcc gcgatcgagc
     1981 cggacgcgga agcgggaccg ccgaagctga aggtgcacac caccgtggac ggctcgcaac
     2041 ggctgctgcg ctcgtggcgg tcgaaggcgt tgccggtggc gatcggggtc ctcgtgctgg
     2101 acctgacgaa gaccgcgaac gccagcccgc tgccgccggt caagcccgtg gtcgcgcatc
     2161 ggctcgccgc caagctcggc aaggccacat cgggggccgc caagctggtc agtgccaagg
     2221 tcgcgatcac gttgatcagc gtcacggcgg tggtggcggc agccgcagcg ctgcacccgc
     2281 aggcgtcgac cggcggaaac ggcccgcagg gacgcgccaa gcagtacggc gatggccaca
     2341 cgatcgaggg tggcggcagc tcggcgacgc gccagatcga gccgccgccg gtgtgcggca
     2401 acggcaaggt ggagcgcgat gagcagtgcg acgacggcaa ccgggtcgac ggcgacggct
     2461 gctcggcggc gtgcatgacg gagccgccgc cggtgtgcgg caacgggaag gtggagcgcg
     2521 gcgaagcgtg cgacgacggc aaccgggtcg acggcgacgg ctgctcggcg gcgtgcatga
     2581 cggagccgcc gccggtgtgc ggcaac
//
LOCUS       DASVOR010002050        13180 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_80276,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002050 DASVOR010000000
VERSION     DASVOR010002050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 13180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13180
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80276"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2063)
                     /locus_tag="VF516_38030"
     CDS             complement(<1..2063)
                     /locus_tag="VF516_38030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HEX8113611.1"
                     /translation="MGVASAIAAAGCAGEAVQGGGRAELDTIQNQTFTAFESGQVRPL
                     ALSADRRFVYATNTPDNRVQIFRIAERRLVPVGSVPVGLEPVALAERGDGELWVVNHL
                     SDSVSVVDVTDPARAHVTRTLLVGDEPRDIVFAGAHRERAFITTAHRGQNTGRDPQLT
                     TPGVGRADVWVFDADHLGASLGGTPLTVITLFADTPRALAVTADGRTVYAAAFQSGNR
                     TTSVHERVITFHGGLPPPLRDAQGVAAPPTSMVVKFRVDPTDHQLHWLDNVGRRWDDH
                     VELSLPDRDVFAIDATANPPVARAGSAFTGVGTVLFNMAVNPVSGKLYVANTDARNDV
                     RFEGHNASGPAQGAPAGSVRGHFAESRITAIDPATGAVTPRHLNKHIDYTVDGTPEEA
                     AKSLAFPTGMAITPDGKTLYVAALGSSKVGVFSTAELETDTFVPSQDRQIALTGGGPT
                     GVALDAQRGMLYVLTRFDNAIAIVDTRTRRQIDRVAMYNPEPSSVVAGRKFLYDATLT
                     SSHGDSACAGCHIFGDFDSLAWDLGDPDGSIEPIPGPFATDPPVPPGFPPFPVVLHSL
                     KGPMATQSLRGLANHGPMHWRGDRTGGTDATLQHVLPSAQPDTGTFDEQTAFKKFNVA
                     FPGLLGRSAPLTDAEMQAFTDYILQVTYPPNPIRNLDNSLTPDQQAGRDFFFSHTADG
                     KELNS"
     gene            2981..3583
                     /locus_tag="VF516_38035"
     CDS             2981..3583
                     /locus_tag="VF516_38035"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX8113612.1"
                     /translation="MLGEVLQFLKDRVNDHLRTTGGWRPTDPEQQAVVFPGSESVDAP
                     DFKLEHVTLLVVNLEEDHTLRSADPNRRALADGTTQHVKPPIIINVYVLFVARFKEYD
                     QSMQHLSRILEFFQSHRVFDHTSGPALDDGIEKLTLELVTQSFLEQNNLWGLLRAAYH
                     PSLLYRIRMIVVSDAQGIAAPGIIDTITIAQPVPAPSGVV"
     gene            3580..4479
                     /locus_tag="VF516_38040"
     CDS             3580..4479
                     /locus_tag="VF516_38040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113613.1"
                     /translation="MKHAAVLELAVSHRFHADGRCRELVFEPTAETRRLLDAHRHLVR
                     SSPGRFVVITPLGAAQPLVPLPAGSSLRFDLRIASDDFALFTDLSALQRTPSPLFSNA
                     QMAVGQTGELALTTRMVRDPKTGADVPAEQPPGVLAEAEIVLRDGDAIGRALPTAGFQ
                     ITFQPRQRRWAFYCITDLAADGGVLNIVDVSGAADALSFGDASRTELTEHPDPSDPIG
                     TQLVAHHPGLRCMRFVSDQAVACSDRARTALELRLDGERVSSPLPNPSLWSFTRRELA
                     APALPEELLFQIVEYRVQPFSHP"
     gene            4517..6406
                     /locus_tag="VF516_38045"
     CDS             4517..6406
                     /locus_tag="VF516_38045"
                     /inference="COORDINATES: protein motif:HMM:NF037565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX8113614.1"
                     /translation="MEEIPKLPPSVAEVSTAIPAFIGYTEKVPASALARVTTLLEFRE
                     LFGGPPRARFTVSDDQLGVVRDTSGPEFLLYYAIQHYFKNGGGPCYVVSAGSYSSGPI
                     ESSALEAGLRRLAQEDEPTLVVQTDAVNLDAAKYYALCDQALTQCHDLGDRFAIFDVK
                     RDAELGKDDKDVLRNRLGMQYLCYGAAYYPYLRTLLSYEYDEADVAILPPPPWVKDLA
                     ANTIRVSYAGSSTTPSVKVTQATTQAALAFSTDGTQITIALGAGTDGKPLAVTGNDLA
                     AAWKTWQDANPTKGFTITAAGTGNDGVTATDTTQLLLQWSRELSGNAIKLTFLGAGTA
                     VPTVAVAFDATAATPRFAIADRALTITVKDATVTGAGVATAWATWKSGVGHDPMGFDL
                     AKAGTGSDGLAAGTVALRRRTLESIRSTDTALYNKIKARLTDELVTLPPSAAIAGIYA
                     RVDRDRGVWKAPANQSLSDVIEPVVKITDAIQDDLNIDTTAGKSINAIRAFTGKGTLV
                     WGARTLAGNDNEWRYVPVRRLFIMIEESAKKSTSFAVFEPNDMTTWLKVKGMIESFLY
                     GLWERGALAGPTPASAYFVNVGLGKTMTTQDILEGRMIVEIGVAAVRPAEFIILRFSH
                     KLQEA"
     gene            6410..6883
                     /locus_tag="VF516_38050"
     CDS             6410..6883
                     /locus_tag="VF516_38050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8113615.1"
                     /translation="MALSKAEIKTAYPLPVYNYRVEIAGVAVGFSEVSGLAIKHEVTT
                     YKESPVGAGSPGPITMHMPAQATPPTITLKKGLVKANSVPTLFAWINATQINQIDKKD
                     VYVRLCDEEGKAVVSWKVLNAFPTSLTAPTFTASSNDAAIESLELRGDRILIEEA"
     gene            6894..7343
                     /locus_tag="VF516_38055"
     CDS             6894..7343
                     /locus_tag="VF516_38055"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8113616.1"
                     /translation="MALPASAQRTSYPLALYNFRVKIAETSMSFTEVSGIAIDHDHVT
                     YRHGLSFAEGEDIATFYFDSFIPITCKRGTILGGDPLFLHDWLNKRDLRRMDVSLCDE
                     TGTPVMSWQIAAAVPVSLKAPGFSAASNDAAIETVELRARGVSLVEL"
     gene            7330..7782
                     /locus_tag="VF516_38060"
     CDS             7330..7782
                     /locus_tag="VF516_38060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8113617.1"
                     /translation="MSSSDLLLGFRFGVYFFAGGVVPNPLDIRFQKVRGLAATVKTTP
                     LSEGGQNLYTQQLPSGIEYGNLVLERGMVVGSPLNIEFNAAMSLFEFAPSNVLVTLFD
                     DAKLPISAWFFTRAYPVKWSTSDLDATQKSVIIDTLELAYARMQIMRI"
     gene            7785..7952
                     /locus_tag="VF516_38065"
     CDS             7785..7952
                     /locus_tag="VF516_38065"
                     /inference="COORDINATES: protein motif:HMM:NF039278.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HEX8113618.1"
                     /translation="MSIEIKELVIRAVVDARPERSAAAAPTASTGEQQDAIVEAAVRE
                     VLRVLRAAKER"
     gene            7956..8672
                     /locus_tag="VF516_38070"
     CDS             7956..8672
                     /locus_tag="VF516_38070"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113619.1"
                     /translation="MGFDLFKLEKLKIAAFDDVARGKRPVLEFEAMFNPASYSQTYAI
                     QWARQQGINSTGAELGYARSLPGELALDLVVDGTGVNEIGVLAATRKTVSERVQELID
                     VTFNYNGAIHQANYLLVTWGKLSFSCRLASLTVNYTRFDRDGTPLRAELKVALVSDES
                     AKKLAKEKQATSPDLTHARIVKSGDTLPLLTKAIYGSSARYLDVARYNDLDDFRNLPA
                     GLEIVFPPLDALAGDPPARR"
     gene            8679..10316
                     /gene="vgrG"
                     /locus_tag="VF516_38075"
     CDS             8679..10316
                     /gene="vgrG"
                     /locus_tag="VF516_38075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEX8113620.1"
                     /translation="MPAVTPTILSDGNPLDPAVELCSIDIRREVDRIPQAELRVLDGD
                     AARRQFAASDSGFFDPGAQIEIKLRYEGEPDATVFKGVVVRQRVEAGHDGSLLVIGLK
                     DHAVKLVAARRYAVFENKTDDAIVKQVISDAGLTAGKVTATQPQHAEMVQYDATPWDF
                     LLSRADAQGLLTVVDDGAISLLPMAISGSPAHRFEYGIDEILDFEIETDASGQFESVE
                     SVGWSAADAKPTDAHKAKSVTASPGDLDGAKLAKALGNGTCTLTHAVPISPDELGAWA
                     DATMARSRLSMCRGRLAIPGLAALKLLDVMEIAGVGKRFAGQTLVTGIRHIVDRKGWR
                     TDVQFGLSPERFSRREQIQDAPAAGLLPGVSGLQIGVVAAFEADPDKELRVRVQLPTL
                     DPTKGLVWARLACPDAGSGRGYYFRPDKDDEVVVGFLNDDPRYPVILGSLYGSKNAPA
                     AAAGTPDDKNQNKGVFTRKGTTLGFVDGDKPSVFIETPNKNKLLLDDDGETVVLTDQH
                     GNSITLSKDGIVLKSAKDLKIDAGGNVEIKGAKVDVK"
     gene            10353..10769
                     /locus_tag="VF516_38080"
     CDS             10353..10769
                     /locus_tag="VF516_38080"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX8113621.1"
                     /translation="MPGDGSFLGRGWAFPPAFTAGGGDVEVVAGADDVQQSLEILFTT
                     APGERVMQDTFGTDLSRVMFEELDQGLANTIERLIKNAIVEHEPRIKLDRVDVENHPD
                     EPYALRIHVQYTVRGTNSRFNLVFPFYRMEAIRPGA"
     gene            10773..11204
                     /locus_tag="VF516_38085"
     CDS             10773..11204
                     /locus_tag="VF516_38085"
                     /inference="COORDINATES: protein motif:HMM:NF039577.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113622.1"
                     /translation="MADLILIDGDTVSFLPSFGAAIVVPQPGKLEGSGPATLGGKPLC
                     VDGDETKVSVAGCMYTTPQYCIPGTGTLKIDALAGDQKATKTTTGGKAVLLRGTTFTA
                     RFEVGSPANQPAPPGPPIPDSTTQYSGSGMFITTNTRFQGT"
     gene            11225..>13180
                     /locus_tag="VF516_38090"
     CDS             11225..>13180
                     /locus_tag="VF516_38090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113623.1"
                     /translation="MLKQPESLPRDGTSQQARTLASLDPGSVPVDERTLPDLVGFARE
                     LGKSLVYYDADNRPAGDWSGFIGSLDPGLVAAFLDNPARFAPTTTPELFRPHFVLFLA
                     FLQLFERARAALNSVTQRHLDFYYRRVLQLAGKGPVADRVHLVLDLARGEREVLVPAG
                     SLVSAGPDSLGRDQVYTTEQPIIVNRIAIARTRTIHVARQRTGFAAARADHPGAPLDA
                     FLAMLGIALGEPLPAFPGATGPLTVAQLVQLAARADGDDETTPRPGLPGQPDPGRFYL
                     TVSEIGDVVRVNALLAADHPEKPSTLAKLLDQSPRRQLALGILHPVVPKVEDIVAYWE
                     ALGSTFRMPPESFHQIMQLLVHPEADPWSEVDALLEAAHQRKVRNDRIGRLREIHAAQ
                     PDAGLLPLVREALGDDAASPADLTSFLDMTALDSLTHAEAASDWETLYSVLELAQRHR
                     TGERPAQKVTWLDIVAADDAPTTAARPAPDGQGSAGRWATFGLPRAANPSAPPAPIIG
                     WAIASPLLWLREGTRTITLILELGRDASAQDAALHDVLTATPCPLMFQVSTARAWVEC
                     SSATIEVDSLATLLEQTPTADDHLAIRCTLKVAGNVDPIAPLADLGAPAVRVMLRPIQ
                     LAAGAPYTTRYTELSALRLVGVRVRVDA"
BASE COUNT         2170 a         4553 c         4429 g         2028 t
ORIGIN      
        1 tccgagttca gctccttgcc gtcggcggtg tggctgaaga agaagtcgcg gccggcctgc
       61 tgatcgggtg tcagcgagtt atcgagattg cggatcggat tgggtggata cgtgacctgc
      121 aggatgtagt cggtgaacgc ctgcatctcg gcgtcggtca gcggcgcgct gcggccgagc
      181 agtccgggga acgcgacgtt gaacttcttg aacgcggtct gctcgtcgaa cgtgccggtg
      241 tcgggctgcg cgctgggcag gacgtgctgc agcgtcgcgt cggtgccgcc ggtgcggtcg
      301 ccgcgccagt gcatcggtcc gtggttggcc aggccgcgca ggctctgggt cgccatcggc
      361 cccttgagcg agtgcaggac caccgggaac ggcgggaatc cgggcggcac cggcggatcg
      421 gtggcgaacg ggccggggat cggctcgatc gacccgtccg ggtcgccgag gtcccacgcg
      481 aggctgtcga agtcgccgaa gatgtggcag ccggcgcacg ccgagtcgcc gtggctcgag
      541 gtcagggtcg cgtcgtacag gaacttgcgg ccggcgacca cgctgctcgg ctcggggttg
      601 tacatcgcga cgcggtcgat ctgccgccgg gtccgggtgt cgacgatcgc gatcgcgttg
      661 tcgaaccggg tcagcacgta cagcatgccg cgctgtgcgt cgagcgcgac gccggtcggg
      721 ccgccgccgg tcagcgcgat ctggcggtcc tgcgacggca cgaacgtgtc ggtctcgagc
      781 tcggcggtcg agaacacacc gaccttgctc gatcccagcg ccgcgacgta cagcgtcttg
      841 ccgtcgggcg tgatcgccat gccggtcggg aacgccaggc tcttcgcggc ctcctccggc
      901 gtgccgtcga cggtgtaatc gatgtgcttg ttgaggtggc gcggagtcac cgccccggtc
      961 gccggatcga tcgcggtgat ccggctctcg gcgaagtggc cgcgcaccga gccggccggc
     1021 gcgccttgcg ccgggccgga tgcgttgtga ccctcgaacc ggacgtcgtt gcgcgcgtcg
     1081 gtgttcgcga catagagctt tccggacacc ggattgacgg ccatgttgaa cagcacggtg
     1141 ccgacgccgg tgaacgcgct gccggcccgg gcgaccggcg ggttggccgt cgcgtcgatc
     1201 gcgaacacgt cccggtcggg caggctgagc tcgacgtggt cgtcccagcg ccggccgacg
     1261 ttgtcgagcc agtgcagctg gtggtccgtc ggatcgaccc ggaacttcac caccatgctg
     1321 gtcggcggcg ccgcgacgcc ctgcgcgtca cgcagcggcg gcggcagccc accgtggaac
     1381 gtgatgactc gctcgtggac cgacgtcgtg cgattgcccg actggaacgc cgccgcgtag
     1441 accgtgcggc cgtcggcggt gaccgcgagc gcgcgcggcg tatccgcgaa cagcgtgatc
     1501 acggtcagcg gtgtgccgcc gagcgaggct ccgaggtggt cggcgtcgaa cacccagaca
     1561 tcggcgcgcc cgacgcccgg cgtcgtgagc tgcggatccc ggccggtgtt ctggccgcgg
     1621 tgcgcggtgg tgatgaacgc gcgctcgcga tgcgcgccgg cgaacacgat gtcgcgcggt
     1681 tcgtctccga ccagcagcgt gcgcgtgacg tgggcccgcg ccgggtccgt gacgtcgacg
     1741 acgctgacgc tgtcggacag gtgattgacc acccacagct cgccatcgcc gcgctcggcc
     1801 agcgcgaccg gctcgagccc gaccggcacc gatccgaccg gcaccaggcg gcgctccgcg
     1861 atccggaaga tctggacacg gttgtcgggt gtattcgtcg catagacgaa tcgccggtcc
     1921 gccgacagcg cgagcggccg gacctgaccg ctctcgaacg cggtgaacgt ctggttctgg
     1981 atcgtatcga gctctgcccg gccgccaccc tggaccgcct cgccggcgca tcccgcggcc
     2041 gcgatcgccg atgcgacacc cactgcactt ccccatcgta aacgcatttg catctccccc
     2101 atggactacc ttcctcaggc tgaccgtcga gtcggatgcc ggcaagtccg atctcgcttc
     2161 acacgatgct ggactgctac gcggatatac aatctgtacc gatagcgatg aatctgcaat
     2221 ctaccggttg ttgccactgc gacatgcgac gcgcgcgcgc cgcaggcacg acgtcgtctg
     2281 aacaccccgg acaccctgtc tcccgcgacg tcccgcgaca ccggcggaac atcggcactg
     2341 gtatcgcgat atgtagtttt ccaggtgggc ggcgcatccg cacgaaggtt cgagaagcgc
     2401 ggtcgctgcg agcggcgccg cgccctgcat gaccgctcct gctagacctg cgcggccggc
     2461 tgctcctcgg gtgcgctctc cggcggtgcg tcgccgcgcg gccgaccggc gatgcctcga
     2521 tcggcccgcg ctccttcgtc ccatgctcga cctgccggtc cgcctgcgag gggtgcaacg
     2581 ctcgatgccc ggtcggcccg tccaggcgaa aaccggcgcg ggaccggggc ggcccgggcc
     2641 gtcgccggcc ggttgtctcc cgaaccggtg cggaagaaga agtcgcggcc ggcctgttgc
     2701 cggcccgcag cgccgggtcg ccgtgtcgcc ggtgtgcgat ccagctgcgg ccctcgtggc
     2761 cgggggcgca gaggatgcgc gatccacagg cctgccgcga ttcgatgcag tcgccacagg
     2821 tctctccacg ttcgaagacg acgagctcgt gagcggcgga ttcctggttt gatgggatat
     2881 cacgggcgct tcctcgtgct cggctcgctt ccgcgacggc gatcgatcgc ggcacgcagt
     2941 ggtgcgtggc acagccgttg cactgcacgc gcccgagcac atgctgggcg aggttctgca
     3001 gttcctgaag gatcgggtca atgatcacct gcggacaaca ggtggatggc ggcccaccga
     3061 tccggaacag caggccgtgg tgttccctgg cagtgagagt gtcgacgcac ccgacttcaa
     3121 actggaacac gtgacactgc tcgtggtcaa cctggaagag gatcacacgc tcagatcggc
     3181 agatccgaat cgcagggcat tggccgatgg cacgacacag cacgtgaaac caccgatcat
     3241 catcaatgta tacgtcctgt tcgtggctcg gttcaaggaa tacgaccaga gcatgcagca
     3301 tctatcgcgc attctcgagt tcttccagag ccatcgcgta ttcgaccaca ccagcggtcc
     3361 ggcgctcgat gacggcatcg agaagctgac cctcgagctc gtgacgcagt cgttcctgga
     3421 gcagaacaac ctgtggggcc tgctgcgcgc ggcctatcac ccgtccttgc tgtatcggat
     3481 ccggatgatc gtcgtcagcg acgcacaggg catcgcggcc ccggggatca tcgacacgat
     3541 cacgatcgcg cagccggtgc cggcgccgag cggggttgta tgaagcatgc ggcagtcctc
     3601 gagctcgccg tgagccaccg cttccacgcg gacgggcgat gtcgcgagct ggtgttcgag
     3661 ccgacggcgg agacccggcg gctgctcgac gcgcatcgcc acctcgttcg ctcgagcccc
     3721 ggtcgcttcg tcgtgatcac gccgctcggg gccgcgcagc cgctcgtgcc gctgccggcg
     3781 gggtcctcgc tgcgcttcga tctgcggatc gcgagcgatg attttgcgct gttcaccgat
     3841 ctgtccgccc tccagcgcac gccctcgccc ctgttcagca acgcgcagat ggcggtgggc
     3901 cagaccggcg agctcgcgct cacgaccagg atggtccgcg acccgaagac cggcgccgac
     3961 gtgccggccg agcaaccacc cggcgtgctc gccgaggccg agatcgtgct gcgcgatggc
     4021 gatgcgatcg gccgcgcgct gcccacggcc ggcttccaga tcacgttcca gcccagacaa
     4081 cggcgctggg cgttttattg catcaccgat ctcgcagccg atggtggagt cctcaacatc
     4141 gtggatgtgt ccggggcagc agatgcgctg tcgttcggcg atgccagccg gaccgagctc
     4201 accgagcatc cggacccgtc cgatccgatc gggacccagc tggtggctca ccaccccggg
     4261 ctgcgctgca tgcggttcgt gtccgaccag gcggtcgcct gcagcgaccg ggcgaggacc
     4321 gcgctggagc tgcgactcga tggcgagcgc gtatcgagcc cactccccaa tccatccttg
     4381 tggagcttca cccggcggga gctcgccgct ccggccctac cggaggagct cctgtttcag
     4441 atcgtcgagt atcgcgtcca acccttttcc cacccctgag tgaacgatgc cgtcctacaa
     4501 aaccccaggc gtatatgtgg aagagatccc gaagctgccg ccctcggtgg ccgaggtctc
     4561 caccgcgatc ccagccttca tcggttacac cgagaaggtc cccgcaagcg cgctcgcccg
     4621 cgtgacgacc ctgctcgagt tcagggagct gttcgggggt ccgcccaggg cccgcttcac
     4681 ggtcagcgat gaccagctcg gcgtggttcg agacacgtcg ggtccggagt tcctcctgta
     4741 ctatgccatt cagcactact tcaagaacgg cggagggccg tgttacgtcg tctcggcggg
     4801 gagctacagc agcgggccga tcgaatcgtc cgcgctcgag gcgggcctcc ggcggctcgc
     4861 ccaggaagac gagcccaccc tcgtggtcca gaccgacgcg gtgaacctgg acgccgcgaa
     4921 gtactatgcg ctgtgcgatc aggcgctcac ccagtgtcat gacctgggag accgctttgc
     4981 gatcttcgac gtcaagcgag acgccgagct cggcaaggac gacaaggatg tactccgcaa
     5041 ccggctcggc atgcagtacc tgtgctacgg agcggcctac tatccctacc tccggaccct
     5101 gctgagctac gagtacgacg aggccgacgt cgccatcctg ccaccgccgc cgtgggtcaa
     5161 ggacctcgcc gccaacacga tccgcgtgag ctacgccggg tcgagcacga caccttccgt
     5221 gaaggtgacc caggcgacga cccaggccgc gctcgcgttc agcacggacg gcacccagat
     5281 cacgatcgcg ctcggggccg gcaccgacgg caagcccctg gccgtgaccg gcaacgatct
     5341 cgccgccgcg tggaagacct ggcaggacgc caatcccacc aagggcttca cgatcaccgc
     5401 cgcggggacc gggaacgacg gcgtgacggc gaccgacacc acccagctcc tgttgcagtg
     5461 gtcgcgcgag ctgtccggca acgcgatcaa gctgacgttc ctcggcgccg gaaccgccgt
     5521 ccccaccgtc gccgtcgcgt tcgacgccac ggctgcgacg ccgaggttcg cgatcgccga
     5581 ccgggcgctc acgatcaccg tcaaggacgc caccgtcacc ggcgccggcg tggccacggc
     5641 gtgggcgacc tggaagtccg gcgtcgggca cgacccgatg gggttcgacc ttgccaaggc
     5701 gggcaccgga agcgacggcc tcgccgcagg gaccgtcgcg ctcaggcgcc gcaccctcga
     5761 gtcgatccgg tcgaccgaca ccgcgctgta caacaagatc aaggcgaggc tgaccgacga
     5821 gctcgtgacg ctcccgccga gcgcggcgat cgccggcatc tatgcccgcg tcgaccgcga
     5881 tcgcggcgtg tggaaagctc ccgcgaacca gagcctcagc gatgtcatcg agcccgtcgt
     5941 caagatcacc gacgcgatcc aggacgacct caacatcgac accaccgccg gcaagtcgat
     6001 caacgcgatc cgcgcgttca ccggcaaggg cacgctggtg tggggcgccc ggaccctcgc
     6061 cggcaacgac aacgagtggc gctacgtccc ggtccggcgg ctgttcatca tgatcgagga
     6121 gtcggccaag aagtcgacga gctttgccgt gttcgagccc aacgacatga ccacctggct
     6181 caaggtcaag gggatgatcg agagcttcct gtacggtctg tgggagcgcg gcgcgctggc
     6241 ggggccgacg cccgcatcgg cgtacttcgt caacgtcggc ctgggcaaga cgatgaccac
     6301 ccaggacatc ctcgagggcc ggatgatcgt cgagatcggc gtcgcggccg tgcggccggc
     6361 ggagttcatc atcctccggt tctctcacaa gctgcaggag gcatgagcca tggcgctgtc
     6421 caaagctgag atcaagacgg cctatccgct gccggtctac aactaccgtg tcgagatcgc
     6481 gggcgtggcg gtcggcttct ccgaggtctc cgggctcgcg atcaagcacg aggtcacgac
     6541 ctacaaggag agcccggtcg gcgccggatc accggggccg atcacgatgc acatgccggc
     6601 ccaggcgacg ccgccgacga tcacgctgaa gaaggggctg gtcaaggcga acagcgttcc
     6661 cacgctgttt gcctggatca acgcgaccca gatcaaccag atcgacaaga aggacgtcta
     6721 cgtgcggctg tgcgatgaag agggcaaggc ggtggtcagc tggaaggtgc tgaatgcatt
     6781 cccgacctcg ctgaccgcgc cgacgttcac cgcgagctcg aacgatgccg ccatcgagag
     6841 cctggagctc cggggggatc gcatcctcat cgaggaagcc tgagaggtga ccgatggccc
     6901 tgccggcgag cgcgcagcgc acgagctacc cgctcgcgct gtacaacttc cgggtcaaga
     6961 tcgcggagac ctcgatgagc ttcaccgagg tctcggggat cgcgatcgac cacgaccacg
     7021 tgacctaccg gcacgggctg agctttgccg agggcgagga catcgcgacg ttctatttcg
     7081 acagcttcat tccgatcacc tgcaagcgcg gcacgatcct cggcggagat ccgctgttct
     7141 tgcacgactg gctgaacaag cgcgacctcc gccgcatgga cgtcagcctg tgcgacgaga
     7201 ccgggacgcc cgtgatgtcc tggcagatcg ccgccgccgt gccggtcagc ctcaaggcac
     7261 cggggttctc cgccgcgtcg aacgatgcgg cgatcgagac cgtggagctg cgagcgaggg
     7321 gggtctccct tgtcgagctc tgatctactc ctgggattcc ggttcggcgt gtacttcttt
     7381 gccgggggcg tggtgcccaa tccgctggat atccggtttc agaaggtgag agggctcgcg
     7441 gccacggtga agaccacgcc gttgtcggaa ggcggccaga acctctatac ccagcagctg
     7501 ccatcgggca tcgagtatgg aaacctcgtc ctcgagcgcg gcatggtggt cggctcaccg
     7561 ctcaacatcg agttcaacgc ggcgatgtcg ctgttcgagt tcgcaccttc gaatgtgctc
     7621 gtcacgctgt tcgacgacgc caagctgccg atctcggcgt ggttcttcac cagggcgtat
     7681 cccgtcaagt ggtccacctc ggacctcgac gccactcaga agagcgtgat catcgacacc
     7741 ctcgagctcg catacgctcg catgcagatc atgaggatat gaccatgtcg atcgagatca
     7801 aggagctggt gatccgcgcc gtggtggacg ccagacccga acggtccgcg gccgccgcgc
     7861 ccacggccag caccggcgag caacaggacg cgatcgtgga agcggcggtc agggaggtac
     7921 tgcgagtgct tcgcgccgcc aaggagcgct gagcgatggg cttcgacctg ttcaagctcg
     7981 agaagctcaa gatcgccgcg ttcgacgacg tcgcgcgcgg caagcgcccc gtcctcgagt
     8041 tcgaggcgat gttcaatccc gcctcgtact cgcagaccta cgccattcaa tgggcgcggc
     8101 agcaagggat caactccacc ggcgccgagc tcggatatgc gcgcagcctg ccgggcgagc
     8161 tcgcgctcga cctcgtggtc gacggcaccg gcgtgaacga gatcggcgtc ctcgcggcca
     8221 cccgcaagac cgtgagcgag cgcgtgcagg agctgatcga cgtcaccttc aactacaacg
     8281 gcgccattca ccaggccaac tacctcctgg tgacctgggg caagctcagc ttcagctgtc
     8341 gcctggcgtc gctgacggtc aactacaccc gtttcgaccg cgatgggacg ccgctccggg
     8401 ccgagctcaa ggtggcgctc gtatcggacg aatctgccaa gaagctggcc aaggagaagc
     8461 aggcgacctc gcccgatctc acgcacgcgc ggatcgtcaa gagcggcgat accctcccgc
     8521 tgctcaccaa ggcgatctat ggctcgtcgg cgcgctacct cgacgtcgcc cgctacaacg
     8581 acctcgacga tttccggaac ctccctgccg gcctcgagat cgtgtttcca cccctcgacg
     8641 cgctcgccgg ggatcccccg gcgcggagat agccagtcat gcccgcggtc acacctacga
     8701 tcctcagcga tggcaatccg ctggatcccg ccgtcgagct gtgctcgatc gacatccggc
     8761 gcgaggtcga ccgcatcccg caggcggagc tgcgcgtgct cgacggcgat gcggcccggc
     8821 ggcagtttgc agccagcgac agcggcttct tcgatcccgg ggcacagatc gagatcaagc
     8881 tgcgctacga gggagagccg gacgccacgg tgttcaaggg cgtcgtggtg cgccagcggg
     8941 tcgaggccgg ccacgacggg tcgctgctcg tgatcgggct caaggaccac gcggtcaagc
     9001 tggtcgccgc ccggcgatat gcggtcttcg agaacaagac cgacgacgcg atcgtcaagc
     9061 aggtgatcag cgacgccggc ctcaccgccg gcaaggtcac ggccacccag cctcagcacg
     9121 ccgagatggt gcagtacgac gccacgccgt gggactttct gctgtcgcgc gcggatgccc
     9181 aggggctgct caccgtggtc gacgatggcg cgatctcgct gctgccgatg gcgatctccg
     9241 gctcgccggc ccatcgcttc gagtacggga tcgacgagat cctcgacttc gagatcgaga
     9301 ccgacgcgag cggtcagttc gagagcgtgg agagcgtcgg ctggagcgcc gcggacgcca
     9361 aaccgacgga tgcccacaag gcgaagagcg tcacggcctc cccgggcgat ctcgatggtg
     9421 cgaagcttgc caaggcgctc ggcaacggca cgtgcacgct gacccacgcc gtgccgatct
     9481 cgccggacga gctgggtgcg tgggccgacg cgacgatggc gaggagccgg ctttcgatgt
     9541 gccgtggccg cctcgcgatc cccgggctcg ccgcgctcaa gctgctcgac gtgatggaga
     9601 tcgccggcgt cggcaagcgg ttcgccggcc agacgctggt gaccggcatc cgccacatcg
     9661 tcgatcgcaa gggctggcgc accgatgtgc agttcggact cagcccggag cggttcagcc
     9721 gcagggagca gatccaggac gcgccggccg ccggcctgct cccgggcgtc agcggtctcc
     9781 agatcggcgt ggtcgccgcg ttcgaggcgg atcccgacaa ggagctgcgc gtcagagtcc
     9841 agctgccgac cctcgatccc accaagggcc tggtgtgggc gcgcctggcc tgtccggacg
     9901 ccggcagcgg ccgcggttac tacttccgtc cggacaagga cgacgaggtc gtcgtcggct
     9961 ttctgaacga cgacccgcgt tatccggtga tcctcggctc gctctacgga tcgaagaacg
    10021 cgcccgcggc ggcggcggga acaccggacg acaagaacca gaacaagggg gtgttcacca
    10081 ggaaggggac cacgctgggc ttcgtcgacg gcgataagcc ttcggtgttc atcgagacgc
    10141 cgaacaagaa caagctcttg ctcgacgacg atggcgagac ggtggtgctg accgatcaac
    10201 acggcaactc gatcacgctg agcaaggacg gcatcgtcct caagagcgcc aaggacctga
    10261 agatcgatgc cggcggaaac gtggagatca agggcgccaa ggtggatgtc aaatgaccgt
    10321 gtacgaacgc gcagatctgg atcgagggtg tcatgccggg tgacggctcg ttcctcggca
    10381 gaggctgggc gttcccgccg gcgttcaccg cgggcggcgg cgacgtggag gtggtcgccg
    10441 gcgccgacga cgtgcagcag agcctcgaga tcctgttcac gaccgcgccg ggtgagcgcg
    10501 tcatgcagga cacgttcggc accgacctgt cgcgcgtgat gttcgaggag ctggatcagg
    10561 ggctcgccaa cacgatcgag cggctgatca agaatgcgat cgtcgagcac gagccgcgga
    10621 tcaagctcga cagggtcgac gtcgagaacc atcccgacga gccgtatgcc ctgcgcatcc
    10681 acgtccagta caccgtgcga ggcaccaact cgcgcttcaa cctggtgttt ccgttctacc
    10741 gcatggaggc catccgcccg ggagcgtgag ccatggcaga cctcatcctc atcgacggcg
    10801 atacggtcag cttcttgccg agcttcggag cggcgatcgt ggtgccgcag ccgggcaagc
    10861 tcgagggcag cggcccggca acgctcggcg gcaagccgct gtgtgtcgac ggcgacgaga
    10921 ccaaggtctc ggtggccggc tgcatgtaca ccacaccgca gtactgcatt cccgggacag
    10981 gcacgctgaa gatcgacgcg ctggccggcg atcagaaggc gaccaagacc acgaccggcg
    11041 gaaaggccgt gctgctccgc ggcacgacgt tcaccgccag gttcgaggtc gggagtcccg
    11101 cgaaccagcc ggcgcctccg gggcctccga tcccggactc gacgacgcag tactccggca
    11161 gcggcatgtt catcaccacc aacacgcggt tccaggggac ctgaacggcg aaccgatgac
    11221 ggccatgctc aaacagccgg agtcgctacc gcgcgacggc accagccagc aggcgcgcac
    11281 cctggcatcg ctcgatccgg gcagcgtgcc ggtcgacgag cggacgctcc cggacctcgt
    11341 ggggttcgcg cgcgagctcg gcaagagcct ggtgtactac gacgcggaca accggccggc
    11401 cggagactgg tccggcttca tcggcagcct ggatcccggc ctggtcgccg cattcctcga
    11461 caacccggcg cggttcgctc cgaccaccac accggagctg ttccgcccgc acttcgtgct
    11521 gttcctcgcg ttcctgcagc tgttcgagcg cgcacgggct gcgctcaact ccgtgaccca
    11581 gcgccatctc gacttctact atcgccgcgt cctgcagttg gccggcaagg ggccggtggc
    11641 ggaccgggtg cacctcgtgc tcgatctcgc ccgtggcgag cgcgaggtcc tggtgccggc
    11701 gggcagcctg gtcagcgccg gaccggacag cctcggccgc gatcaggtct acacgaccga
    11761 gcaaccgatc atcgtcaacc ggatcgcgat cgccaggacc cggacgatcc acgtcgcgcg
    11821 ccagcgcacc gggttcgcag cggccagggc cgaccacccc ggcgcgccgc tggatgcgtt
    11881 cctcgcgatg ctggggatcg cgctcggcga gccgctgccc gcgttcccgg gcgcgacggg
    11941 cccgctcacc gtcgcgcagc tcgtgcagct cgccgcgcgg gccgatgggg atgacgagac
    12001 cacgcccagg ccggggctgc ccggccagcc cgatcccggt cgcttctacc tgacggtgtc
    12061 cgagatcggc gacgtggtcc gcgtgaacgc gctgctggcc gcggatcatc ccgagaagcc
    12121 gagcacgctg gccaagctcc tcgaccaatc tccccgccgc cagctcgcgc tcggcatcct
    12181 gcatccggtg gtcccgaagg tggaagacat cgtggcgtac tgggaagcgc tcggcagcac
    12241 cttccgcatg ccgccggaga gcttccacca gatcatgcag ctgctggtcc accccgaggc
    12301 cgatccatgg agcgaggtgg acgcgctcct cgaggccgcg catcagcgca aggtccgcaa
    12361 cgaccggatc ggccggctcc gcgagatcca cgcggcgcag ccggacgccg ggttgctgcc
    12421 gctcgttcgc gaggcgctgg gcgacgacgc cgcgagcccg gccgacctga cctcgttcct
    12481 cgacatgacc gcgctcgact cgctcaccca cgcggaagcg gcgagcgact gggagaccct
    12541 ctacagcgtc ctcgagctcg cacagcgcca ccggaccggc gagcgcccgg cgcagaaggt
    12601 gacctggctc gacatcgtgg ccgccgacga tgcgccgacc accgcggctc gcccggcgcc
    12661 cgatggacag ggcagcgccg ggcggtgggc gacgttcgga ttgccgcgcg ccgcgaaccc
    12721 gagcgccccg cccgctccga tcatcggctg ggcgatcgcg tccccgctgc tgtggctccg
    12781 cgagggcacg cgcacgatca cgctgatcct cgagctcggc cgggatgcgt cggcgcaaga
    12841 cgccgcgctc cacgacgtgc tcaccgcgac gccctgtcca ctcatgttcc aggtcagcac
    12901 ggccagggcc tgggtcgagt gctcgagcgc gacgatcgag gtcgactcgc tcgccacgct
    12961 gctcgagcag acgcccaccg ccgacgatca cctggcgatc cgatgcacgc tgaaggtcgc
    13021 gggcaacgtc gacccgatcg cgccgctcgc ggacctcggc gctcccgccg tgcgcgtgat
    13081 gctgcggccg atccagctcg ccgccggggc gccgtacacc acccggtaca ccgagctctc
    13141 tgcgctccgg ctggtcggcg tgcgcgtgcg ggtggacgcc
//
LOCUS       DASVOR010002051         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_518089,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002051 DASVOR010000000
VERSION     DASVOR010002051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518089"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..619)
                     /locus_tag="VF516_38095"
     CDS             complement(<1..619)
                     /locus_tag="VF516_38095"
                     /inference="COORDINATES: protein motif:HMM:NF019845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEX8113624.1"
                     /translation="MRAIAITKPGGPDVLTLVDRPVPEPSRGEVRIRVRATAVNRADL
                     LQRMGAYPAPADSPPDIPGLEFAGEVDALGPGVERLAVGDRVFGLAGGGGYAEQLVSH
                     ERAVARIPDGMSFEHAAAVPEAFITAHDAIVGQAGLRGGETLLVHAVGSGVGTAAVQL
                     GRALGVFVIGTARTADKLDRARALGMNAGVVPDGSKFADAVRKHSE"
     gene            complement(646..888)
                     /locus_tag="VF516_38100"
     CDS             complement(646..888)
                     /locus_tag="VF516_38100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113625.1"
                     /translation="MSNLLTPVDLEAVTAGGPITPAASSSTDSLLQTLTSLQTTIQNI
                     GNTANQTGFSTTDVLLLGLLLSQRPQTVFVHRPFWW"
     gene            1272..1835
                     /locus_tag="VF516_38105"
     CDS             1272..1835
                     /locus_tag="VF516_38105"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8113626.1"
                     /translation="METFVQGWGLTIVLCGFILLVFLAGWWHVHAELAWVRALTDYLG
                     EGLVAEDAADARPPSSLAVQVIRDESHAILEASDPAWAQKQVRSWQMRAQRLEPALAF
                     WTDLLRQLGLLGTVLGLGLSLAYTGSDLTKLLAPLSLKVWTTVAGLACSTLLSASFSM
                     RLTAWVDACEKNLEAWDARRRARRGPT"
BASE COUNT          249 a          655 c          721 g          242 t
ORIGIN      
        1 gctcgctgtg cttgcggacc gcgtcggcga acttgctgcc gtcggggacc acgccggcat
       61 tcatgccgag cgcgcgggcg cggtccagct tgtcggcggt gcgcgcggtg ccgatcacga
      121 acacgccgag cgcgcggccg agctgcaccg cggccgtgcc gacgccgctg ccgacggcgt
      181 ggaccagcag ggtctcgccg ccgcgcaggc cggcctggcc gacgatcgcg tcgtgtgcgg
      241 tgatgaacgc ctcggggacc gccgcggcgt gctcgaacga catcccgtcc gggatcctgg
      301 cgaccgcgcg ctcgtggctg accagctgct cggcgtagcc gccgccgccg gcgaggccga
      361 acacgcgatc gccgaccgcg agccgctcga cgccgggccc gagcgcgtcg acctcgccgg
      421 cgaactccag gcccgggatg tcgggcggtg agtcggccgg cgcggggtag gcgcccatgc
      481 gctgcagcag gtcggcgcgg ttgaccgccg tcgcccgcac ccggatccgg acctccccgc
      541 gcgagggctc gggcacggga cggtcgacga gggtgaggac gtcgggtcct cctggcttgg
      601 tgatcgcgat cgctctcatg cgggaaccat cccgcaagag cggggtcacc accagaaggg
      661 acggtggacg aaaaccgtct gcgggcgctg gctgagcaag aggccgagca gcaggacgtc
      721 ggtggtcgag aacccggtct ggttcgcggt gttaccgatg ttctggatcg tggtctgcag
      781 cgacgtcagg gtctggagca gcgagtcggt gctgctgctg gcggcgggcg tgatggggcc
      841 gccggcggtg acggcttcga gatcgacggg cgtgagcagg ttcgacatga cgcgggtcct
      901 tgtggtgtac cgacggtcag agcagccggc gtgccaggcg taagtctgca ggccgccgtg
      961 gggcgaaccg gggcgatcgc ggtgctcgag gtgacagccc gccaacccag ggtgacagcg
     1021 catcgccacc cgcggacatc gccaccggca ggcggcgagg ccccgaggcg acgccggcgc
     1081 gcgatgcgca cccgacggaa tgcagccgcc ggccccgggg gcgtggcccc agggaggcgt
     1141 ggccggcgga acgcgtggcc ccgaggaggc atgccgcgca agaccggaac acccgcccgg
     1201 gggcgcgagc ccacagccca tccggaccgg cgccgggacc ggcccggacc gtcatgtaca
     1261 atcgccgcgc gatggagacg ttcgtccagg gctggggcct cacgatcgtc ctgtgcggct
     1321 tcatcctgct cgtgttcctc gccggctggt ggcacgtcca cgccgagctg gcctgggtcc
     1381 gcgcgctgac cgactacctc ggcgaggggc tggtcgccga ggacgcggcc gacgcccggc
     1441 cgccgtcgtc gctcgcggtc caggtgatcc gcgacgagtc gcacgcgatc ctggaggcca
     1501 gcgacccggc ctgggcccag aagcaggtcc gcagctggca gatgcgcgcg cagcggctcg
     1561 agccggcgct ggcgttctgg accgacctgt tgcgccagct cggcctgctc ggcaccgtgc
     1621 tcggcctcgg cctgtcgctc gcgtacaccg gcagcgacct caccaagctg ctcgcgccgc
     1681 tgtcgctcaa ggtgtggacc acggtcgccg gcctggcgtg cagcaccctg ctgtcggcga
     1741 gcttcagcat gcggctgacc gcgtgggtcg atgcctgcga gaagaacctg gaggcgtggg
     1801 acgcccggcg gcgcgcccgg cgaggcccga catgagcggc gcagctcgcc gccgaggatc
     1861 gtgatgc
//
LOCUS       DASVOR010002052         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_400895,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002052 DASVOR010000000
VERSION     DASVOR010002052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400895"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1010
                     /locus_tag="VF516_38110"
     CDS             <1..1010
                     /locus_tag="VF516_38110"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8113627.1"
                     /translation="RGARGARGARGARGAMMTAPARAAMYPFQDRLPAPVYWIYRAFR
                     TLTVALCFAGFWSGAVVIGWLWLPVLWLWPGERAARIRRVHRTVRSGFRLFHVVMRAL
                     RLYHRTSPAARLRPGDAPVVLVANHPTLCDVTSIASLFPGVVAVAGPQYAANPLLGRL
                     VRACGFVATGSHMLRECEARLRMGFDVLVFPEGTRSPLGGGLQPFHRGAFELAARAKV
                     PIVLLKLTCVPPALSKRLPIWKVADRMAVLTIEPFDVIYPAALGSAALGSAALGSAAL
                     GSAALGSAALGSAGLGTDSRALCRAIEQRYCDMFGYPAPEVRAPSAVPTSEPQPLGDS
                     P"
     gene            1007..1303
                     /locus_tag="VF516_38115"
     CDS             1007..1303
                     /locus_tag="VF516_38115"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8113628.1"
                     /translation="MTTTTSDLHHELKLLIVEALQLEDVDPASISDTAPLFGAGLELD
                     SIDALELATAIARKYDVEVNQDQETRDAFRSVSHLAQFITRKRAAAGARASRAG"
     gene            complement(1300..>1876)
                     /locus_tag="VF516_38120"
     CDS             complement(1300..>1876)
                     /locus_tag="VF516_38120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113629.1"
                     /translation="APGLARANDQPPPYPPPPYQPPPGSAPPAGAPPGYGPPAYAPQP
                     GVPVYGPEEITDLDDSAPVPYGYTRVQRTRKGLIIGGAVTLGATYLATAFIGAVAADF
                     LKAEGSNASVGPVFLPVAGPFLEIGQTDSAIARFYLAVSGLGQTAGAIMLLYGLTSPR
                     TVLVRNDQLIVTSLAPMIAPGASGLAVVGRF"
BASE COUNT          220 a          687 c          684 g          285 t
ORIGIN      
        1 cccggggtgc ccggggtgcc cggggtgccc ggggtgcccg gggtgccatg atgaccgccc
       61 cggcccgcgc ggcgatgtac ccgttccagg accgcctgcc ggccccggtc tactggatct
      121 accgcgcgtt ccgcacactg accgtcgcgc tgtgcttcgc cggcttctgg tcgggcgcgg
      181 tcgtgatcgg ctggctgtgg ctccccgtgc tgtggctttg gcccggcgag cgagccgccc
      241 ggatccggcg cgtccatcgc accgtgcgga gcggcttccg gctgttccac gtcgtgatgc
      301 gcgcgctgcg gctgtaccac cgcacgagcc cggccgcccg cctgcgtccc ggcgacgcgc
      361 cggtcgtgct ggtcgccaac caccccacgc tgtgcgacgt gacctcgatc gcgtcgctgt
      421 tccccggcgt cgtcgcggtc gccggcccgc agtacgcggc caacccgctg ctcggccgcc
      481 tggtccgcgc ctgcggcttc gtggcgaccg gcagccacat gctccgcgaa tgtgaggcgc
      541 ggctgcgcat gggcttcgac gtgctggtgt tcccggaggg cacgcgctcg ccgctgggcg
      601 gcgggctgca accgttccac cgcggcgcat tcgagctcgc ggcccgggcc aaggtcccga
      661 tcgtcctgct gaagctgacc tgcgtgccgc ccgcgctgtc caagcggctg ccgatctgga
      721 aggtcgccga tcgcatggcg gtgctgacga tcgagccgtt cgacgtgatc tatcctgcgg
      781 ccctgggctc tgcggccctg ggctctgcgg ccctgggctc tgcggccctg ggctctgcgg
      841 ccctgggctc tgcggccctg ggctccgcgg gcctcggcac ggacagccgg gcgctgtgtc
      901 gcgcgatcga gcagcgttat tgcgacatgt ttgggtatcc tgctcctgag gtacgtgcgc
      961 cttcggccgt cccgaccagc gagcctcaac ctctcggaga ttccccgtga ccacgaccac
     1021 gtccgacctg caccacgagc tcaagctgtt gatcgtcgaa gcgctccagc tggaggacgt
     1081 cgaccccgcc tcgatctcgg acaccgctcc gctgttcggc gccggcctgg agctcgactc
     1141 gatcgacgcg ctcgagctcg cgacggcgat cgcccgcaag tacgacgtcg aggtcaacca
     1201 ggaccaggag acccgcgatg cgttccgctc ggtgtcccac ctggcgcagt tcatcacgag
     1261 gaagcgcgcc gcggccggtg cgcgcgccag ccgcgccggc tagaaccgcc cgacgaccgc
     1321 aagccccgag gcgccggggg cgatcatcgg cgcgagcgag gtgacgatca gctggtcatt
     1381 gcgcacgagc acggtccgcg ggctggtgag cccgtagagc agcatgatgg ccccggcggt
     1441 ctgcccgagg cccgacacgg ccaggtagaa ccgcgcgatc gcgctgtcgg tctgcccgat
     1501 ctcgaggaac ggaccggcga ccgggaggaa gaccgggccg acgctggcgt tcgatccctc
     1561 ggccttgaga aagtcggcgg ccaccgcgcc gatgaacgcc gtcgcgaggt aggtcgcgcc
     1621 gagcgtcacg gcgccgccga tgatcaggcc cttgcgcgtt cgctggacgc gggtgtagcc
     1681 gtacggcacc ggcgcgctgt cgtccaagtc cgtgatctcc tcggggccgt agaccggcac
     1741 gccgggctgc ggcgcgtagg ccggaggccc gtagcccggc ggagcgccgg ccggaggcgc
     1801 gctgcccggc ggcggctggt agggtggcgg cgggtacggc ggcggctgat cattggcccg
     1861 ggccaggccg ggcgcg
//
LOCUS       DASVOR010002053         2041 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_314379,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002053 DASVOR010000000
VERSION     DASVOR010002053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2041)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2041)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2041
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314379"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..202)
                     /locus_tag="VF516_38125"
     CDS             complement(<1..202)
                     /locus_tag="VF516_38125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113630.1"
                     /translation="MLRRRDLELTLVAHRPEEPAAFAAISAEDPNGAGLAVIASQLSP
                     PELGLVELMLREAALDVATALRD"
     gene            complement(356..1018)
                     /locus_tag="VF516_38130"
     CDS             complement(356..1018)
                     /locus_tag="VF516_38130"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEX8113631.1"
                     /translation="MHRRELVIGHTVLGRPIEAIHFTPPSYARPRAPALLFGAIHGDE
                     AVTQLMLERLADELIERPPGRDTWIIPCLNVDGVLAGTRNNANDVDLNRNFASTNWGT
                     ARRPGYHPGAAPEDQPETRALVELIDRVAPDRLVALHSTFRMVNWDGRGERLAAEMSE
                     RCGYPVTGDMGYPTPGSFGTKYGIERNLEVVTLEVPYLDVDEQAWLDCRTALRWCVDL
                     PE"
     gene            complement(1115..>2041)
                     /locus_tag="VF516_38135"
     CDS             complement(1115..>2041)
                     /locus_tag="VF516_38135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113632.1"
                     /translation="QAQARTMPAPAPVPARPVPIMAIPAAQPPPYLASQTASRLIRPI
                     EPWRDGLRAWMFLGGLALLAAFAVPLQTSPALTFHWNLILDGQGTARLPPLILAAVGF
                     LGVVLAIIPMPVAPRGVIATLLGLAGIAVPIALVGVPPWQVLAPLIGVVLLVPSLLVR
                     NEYRDALVARLFVTLGVIGILLPFGLPQNGAIPLVSVFKQLIDLPGTQKVEPALALCL
                     ITIVVMSLLAWLPAPVTGGAKVWAWLLILWALFVHAIHVALAGGIGAAITGAPNQTIL
                     AWVAGGSGHLVGSAYLVIIGYGLASVIGKQLE"
BASE COUNT          319 a          701 c          773 g          248 t
ORIGIN      
        1 ggtcgcgcag cgcggtggcg acatcgagcg cggcctcgcg cagcatcagc tcgaccagcc
       61 cgagctcggg cggcgacagc tgcgacgcga tcaccgccag gccggcgccg ttggggtcct
      121 cggccgagat cgcggcgaac gcggccggct cctccggccg gtgcgcgacc agggtcagct
      181 cgaggtcgcg gcggcgcagc acgccccgca cgtgtcgcag gacgcgaagg tcgaggccag
      241 accaccaggg agattgcacg ccagtccagt gtgacaagtc gtcacaatcg ttggcaagac
      301 ctctcgctgc gacggcggcc gacgaaacaa ttcgtatcag tccccgtcgc cctggctatt
      361 cgggtaggtc gacgcaccac cgtagcgccg tgcggcagtc gagccaggct tgctcgtcga
      421 cgtcgaggta cgggacctcg agggtgacga cctcgaggtt gcgctcgatg ccgtacttgg
      481 tgccgaacga gcccggcgtg ggatagccca tgtcgccggt gactggatag ccgcagcgct
      541 ccgacatctc ggccgcgagg cgctcgccgc ggccgtccca gttcaccatc cggaacgtgg
      601 agtgcagcgc gaccaggcga tcgggcgcga cgcgatcgat cagctcgacg agcgcccggg
      661 tctcgggctg gtcctcgggc gcggcaccgg ggtggtagcc ggggcgccgc gcggtgcccc
      721 agtttgtcga ggcgaagttg cggttgaggt cgacgtcgtt ggcgttgttg cgggtcccgg
      781 cgagcacgcc gtcgacgttg aggcacggga tgatccaggt gtcgcgcccg ggcggccgct
      841 cgatcagctc gtcggcgagc cgctcgagca tgagctgggt gacggcctcg tcgccgtgga
      901 tcgcgccgaa cagcagggcc ggtgcgcgcg gccgcgcgta cgacggcggc gtgaagtgga
      961 tcgcctcgat cgggcggccg agcacggtgt ggccgatcac gagctcgcgg cgatgcatgg
     1021 cgacctcatg cgtcctgctt gccacccgcg gcgaccgcgg gcgacccgaa ggcgaccccc
     1081 aaaggcgtgc ccgaagacgt gcatcgacgc gagctcattc gagctgcttg ccgatcaccg
     1141 aggccaggcc gtagccgatg atcacgaggt acgccgagcc caccagatgg ccggagccgc
     1201 cggcgaccca ggcgaggatc gtctggttcg gcgcgccggt gatcgccgcc ccgatgccgc
     1261 cggcgagcgc gacgtggatc gcgtggacga acagcgccca caggatcagc agccacgccc
     1321 agaccttggc gccgccggtc acgggcgccg gcagccaggc gagcagcgac atcacgacga
     1381 tcgtgatcag acagagcgcc agcgcgggct cgaccttctg cgtgccgggc aggtcgatca
     1441 gctgcttgaa cacgctgacc agcgggatcg ctccgttctg cggcaggccg aacggcagca
     1501 ggatgccgat cacgccgagc gtgacgaaca gccgcgcgac cagggcgtcg cggtactcgt
     1561 tgcgcacgag caggctcggc acgaggagca cgacgccgat cagcggcgcc aggacctgcc
     1621 acggcggcac gccgaccagc gcgatcggga ccgcgatccc ggccaggccg agcagcgtgg
     1681 cgatcacccc gcgcggcgcg accggcatcg ggatgatggc gagcacgacg ccgaggaagc
     1741 cgaccgcggc gaggatcagc ggcggcagcc gcgcggtgcc ctggccgtcg aggatcaggt
     1801 tccagtggaa cgtcagggcg ggcgaggtct gcagcggcac ggcgaacgcg gcgagcagcg
     1861 cgaggccgcc gaggaacatc cacgcgcgca ggccgtcgcg ccacggctcg atcggccgga
     1921 tcagccgcga cgcggtctgg gaggcgaggt acggcggcgg ctgggccgcc gggatcgcca
     1981 tgatcggcac cggccgcgcg ggcacgggcg ccggggcggg catcgtcctc gcctgggcct
     2041 g
//
LOCUS       DASVOR010002054         2539 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_110335,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002054 DASVOR010000000
VERSION     DASVOR010002054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110335"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..1454
                     /locus_tag="VF516_38140"
     CDS             270..1454
                     /locus_tag="VF516_38140"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113633.1"
                     /translation="MVRLVVAGALVLAPACGGGEAVVKTVKKDPRQKTAKQLVVEARD
                     QATAGQNDAADRSYGEAYASASENKKLAFEILKEWVEFLLQSGRRNRACAVAKQYYET
                     NADDTKGYALYADASIANNDGPTALKVTGELVQLTPDDAGAHERRGRALLLAEKTEEG
                     LEELRKAVQLEPASARYHMSLGTALHQTGDYNKAALEFRAALKNAPDDPEAHVLLGMA
                     LRDQSELDESKTYLDRAIALDPRNGRAYFELGVLYNKQLKQAEAEDAFGKAVKYSPND
                     SRFWYAYGEIYRVQDKIDDAVQAYRHAVDLQPPFPKATGKLGSMLVEKKQYDDAEQYL
                     IAAIRQDPKSPANYWYLGKAYAAKHRNRPAIENYELFLKYAPAKDPDREKAKEIINRL
                     RR"
     gene            1581..2312
                     /locus_tag="VF516_38145"
     CDS             1581..2312
                     /locus_tag="VF516_38145"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEX8113634.1"
                     /translation="MTDSIHISADIPAAARELEGRSPTEILRWASQRLGNKLTFATGF
                     GAEGCVIIDLIARHDLPIDLFTLDTGLLFPETYELWRRLESRYGVAIRAVRPHHSVEQ
                     QADEHGSRLWERDPDRCCELRKVVPLRNALAGFDAWITAIRRDQTPERAHARVVEHDR
                     KFGLIKINPLVAWTHDDVWGHLYAHDVPYNPLHDRGYPSIGCHPCTAVVLPGEDARAG
                     RWRGRNKKECGLHVIQPAATVPDAA"
     gene            2314..>2539
                     /locus_tag="VF516_38150"
     CDS             2314..>2539
                     /locus_tag="VF516_38150"
                     /inference="COORDINATES: protein motif:HMM:NF024638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8113635.1"
                     /translation="MSASDALPAAPRPEASAEPGLYPVFLRLDGLPVVVVGGGPVAAG
                     KLDGLLEAGAHVTVIAPVIGEAIRTRAAAHR"
BASE COUNT          428 a          924 c          847 g          340 t
ORIGIN      
        1 cttcgcccgc cggctgcgcc gcgcgccctg ctcggcgatg gcgcggctgc atcccccgga
       61 gtgatcgcgt tgatccagag gggaccgatc atcccgtcgg ccggcggctg gcccggcaga
      121 gccgggccat tcacccctcc gcgcgtcgcg tcgcgcgggt cgatcatctt ccttccagta
      181 tccagcttcc agcatcgccc gggcatcggg agtgctgcgc ggtggcgtgc gcgcgccggt
      241 ttcgctacct tccctgcatg ttcacggcca tggtccggct cgttgtcgcc ggcgcgctcg
      301 tcctcgcgcc ggcctgcggt ggcggtgaag cggtcgtcaa gacggtcaag aaggacccca
      361 ggcagaagac cgccaagcag ctcgtcgtcg aggcgcgcga tcaggcgacg gccggccaga
      421 acgacgcggc ggaccgctcg tacggcgagg cctacgccag cgcgagcgag aacaagaagc
      481 tggcgttcga gatcctgaag gagtgggtcg agttcctgct ccagtccggc cggcgcaacc
      541 gggcgtgcgc ggtcgccaag cagtactacg agaccaacgc cgacgacacc aagggctacg
      601 cgctgtacgc cgacgcgtcg atcgccaaca acgacggccc gacggcgctc aaggtcaccg
      661 gcgagctggt ccagctcacc cccgacgacg ccggcgccca cgagcgccgg ggccgcgcgc
      721 tgctgctcgc ggagaagacc gaggagggcc tcgaggagct gcgcaaggcg gtccagctcg
      781 agccggccag cgcgcggtac cacatgtcgc tcggcacggc gctgcaccag accggcgact
      841 acaacaaggc cgcgctcgag ttccgcgcgg cgctcaagaa cgcgcccgac gaccccgagg
      901 cccacgtgct gctcggcatg gcgctgcgcg atcagagcga gctcgacgag tccaagacct
      961 acctcgaccg cgcgatcgcg ctcgacccca ggaacggccg cgcctacttc gagctcggcg
     1021 tgctctacaa caagcagctc aagcaggccg aggccgagga cgcgttcggc aaggcggtga
     1081 agtactcgcc caacgactcg cggttctggt acgcgtacgg cgagatctac cgggtccagg
     1141 acaagatcga cgacgcggtc caggcgtacc gccacgccgt cgacctccag ccgccgttcc
     1201 ccaaggcgac cggcaagctc ggcagcatgc tggtcgagaa gaagcagtac gacgatgccg
     1261 agcagtacct gatcgccgcg atccgccagg accccaagag cccggcgaac tactggtacc
     1321 tgggcaaggc ctacgccgcc aagcacagga accgcccggc gatcgagaac tacgagctgt
     1381 tcctcaagta cgccccggcg aaggatcccg accgcgagaa ggccaaggag atcatcaacc
     1441 ggctgcgccg gtaggtcccg cgcccgtatg ccgcaactcg tttgaaacaa acaagatttc
     1501 gaggcagaat aagtagcatc cgttatcgtt gacagttcgt ccgctcaggg tatatccaga
     1561 atatccgtta tggcggacgt atgaccgact ccatccacat ctcggctgac atccccgctg
     1621 cggcccgcga gctcgaaggc cgatccccga ccgagatcct ccgctgggcc agccagcgcc
     1681 tcgggaacaa gctgacgttc gctaccggct tcggcgccga gggctgcgtg atcatcgacc
     1741 tgatcgcgcg ccatgacctg ccgatcgacc tgttcacgct cgacaccggc ctgctgttcc
     1801 ccgagaccta cgagctgtgg cggcggctgg agagccgcta cggggtcgcg atccgcgcgg
     1861 tccgccccca ccacagcgtc gagcagcagg ccgacgagca cggctcccgg ctgtgggagc
     1921 gcgacccgga tcgctgctgc gagctgcgca aggtcgtgcc gctgcgcaac gcgctcgccg
     1981 ggttcgacgc gtggatcacc gcgatccgcc gcgaccagac gcccgagcgc gcgcacgccc
     2041 gcgtggtcga gcacgatcgc aagttcggcc tgatcaagat caacccgctg gtcgcgtgga
     2101 cccacgacga cgtgtggggc cacctctacg cgcacgacgt gccgtacaac ccgctgcacg
     2161 atcgcggcta cccgtcgatc ggctgtcacc cgtgcaccgc ggtggtgctg cccggcgagg
     2221 acgcgcgcgc cggccggtgg cgcggccgga acaagaagga gtgcggcctg cacgtgatcc
     2281 agcccgccgc cacggtgccc gatgcagcct gaggtgagcg cgagcgatgc gctgcccgca
     2341 gcgccccggc ccgaggcgtc ggccgagccc gggctctatc cggtgttcct gcggctcgac
     2401 ggcttgccgg tcgtcgtcgt cggtggcggc ccggtcgccg ccggcaagct cgacggcctg
     2461 ctcgaggccg gcgcccacgt cacggtgatc gcgccggtga tcggcgaggc gatccgcacg
     2521 cgcgccgcgg cccaccgcc
//
LOCUS       DASVOR010002055         2125 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_548190,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002055 DASVOR010000000
VERSION     DASVOR010002055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548190"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            290..478
                     /locus_tag="VF516_38155"
     CDS             290..478
                     /locus_tag="VF516_38155"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEX8113636.1"
                     /translation="MTTITEGSKQAVLEQLTAEHRRLDEQLKMLERRRALTPSEQIEI
                     VRLKKQKLLTKDRIVRLS"
     gene            508..1041
                     /locus_tag="VF516_38160"
     CDS             508..1041
                     /locus_tag="VF516_38160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113637.1"
                     /translation="MTSHAAPDVVTPGLTEEMIEKMRAIGLRLDRSGTFWHQGTAVTH
                     PRLRQALLRWLDVRDDGRDIVRLDDRRYAYVEVDDAHLRALGARWDGARCIVHWDDDT
                     ESELDYGSLRQAADHALYAAVRGRLRGRIAGPAYHAIAERIVEAPGEAPGEAPGEAPG
                     GFALDAAGQRWPIAGAA"
     gene            complement(1415..1675)
                     /locus_tag="VF516_38165"
     CDS             complement(1415..1675)
                     /locus_tag="VF516_38165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113638.1"
                     /translation="MAIPGDGERLDVAGASGVQPRGEPVRSSGEAFPTLDEAMRRHIE
                     AALIRCKGRIEGERGAARLLAIHANTLRSRMHKLGIRWERFR"
     gene            1812..>2125
                     /locus_tag="VF516_38170"
     CDS             1812..>2125
                     /locus_tag="VF516_38170"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HEX8113639.1"
                     /translation="MGLTALLVLAVTSFAAATVNGALGYGYSSISVPIALLVTVGRVL
                     NPALVIVEVGINLYALYWNRRTAGRVIRRVIPLAIGLVPGVIAGALLLGHIEPTTMKV
                     IV"
BASE COUNT          327 a          742 c          723 g          333 t
ORIGIN      
        1 cgccgtcggg ccgcgcggtg gcccgcctcg cgctcgccgg cgcccgggtc gacatcaccc
       61 tcggcggcgc gccgacttac atccgtccgt cggaggccga ggtgaaatat cccgacggtg
      121 tgccgggcgc gctgcgccgc cgtgacacgt gatgcacgcg cacatccaga cctttcgggt
      181 gaacgtttcc gggaggtaac tagcgggccg gtggacaacc ggatgattgc cgagcccgga
      241 tcggcggctt ataaaagatc cagatgcagg tccccgccct ggaggctcag tgacgaccat
      301 caccgagggt tcgaagcagg ctgtgctgga acaattgacc gccgaacacc gtcgactcga
      361 cgagcagctg aagatgctgg aacggcggcg cgccctcacc ccctcggaac agatcgaaat
      421 tgtccggctg aagaagcaga agctgctcac caaggaccgc atcgtgcgac tgtcgtgatc
      481 ccggcctgat cgcggcatag tcggcgggtg accagtcacg cggcacccga cgtggtaacc
      541 cccggtctca cggaggagat gatcgagaag atgcgggcga tcggcctgcg tctggatcgg
      601 tccgggacgt tctggcacca gggcaccgcg gtgacccacc cgcggctccg ccaggcgctg
      661 ctgcgctggc tcgacgtccg cgacgacggc cgcgacatcg tgcggctgga cgaccggcgc
      721 tacgcctacg tcgaggtcga cgacgcccac ctccgggcgc tcggggcgcg ctgggatggc
      781 gcccgctgca tcgtgcactg ggacgacgac accgagagcg agctcgacta cggctcgctg
      841 cgccaggccg ccgaccacgc gctgtacgcc gcggtgcgcg gccggctgcg cggccggatc
      901 gccgggccgg cctaccacgc gatcgccgag cggatcgtcg aagcccccgg tgaagccccc
      961 ggtgaagccc ccggcgaggc ccccggcggc ttcgcgctcg acgccgccgg gcagcgctgg
     1021 ccgatcgccg gcgccgcgtg acgcgcccgc tccgatgatt cgtcacaagt cccgacatgg
     1081 cgttcgtgat cgtcgacgtc gacctgatcg ttgacccaga gcgtcgaggt tgacggcaac
     1141 gtacgacgtt gagcccacca tcgacgttgg acgtcgaccg atcctgtcct gcgagtctcg
     1201 ccggagcgcg acaacggggc ggggtcgagg tcgacggtgg agtccaacgt ctacgtatcg
     1261 cttcgctgcc gtcaaactcg acgctcgagt caaggtcgac gtcatcgtca acgttcgcag
     1321 gcccggcgat gttcggcgca ccccagtgcg ggggagttcc acgccggtgg catgcagctc
     1381 gtcgcagtga tacgcgtgtg agctcctgcc tgcgctaccg gaagcgctcc cagcggatgc
     1441 cgagcttgtg catccgcgag cgcagcgtgt tggcgtggat cgcgagcagc cgtgccgcgc
     1501 cgcgctcgcc ctcgatccgg cccttgcagc ggatcagcgc ggcctcgatg tggcggcgca
     1561 tcgcctcgtc gagcgtgggg aacgcctcgc cggacgaccg caccggctcg ccgcgcggct
     1621 gaactccgga cgcaccggcc acgtcgagcc gctcgccatc gccggggatc gccatgggcc
     1681 gaagataggc cctgcgcgcc ccgaggtcac gcggttttcc acgaaatatc gtgggtagct
     1741 aatataacgg atatgaggta cacatccgtt ataacgtcgg ttcggctgct gccactcgcc
     1801 aggggtatcc gatgggtctc actgctctcc tcgtcctcgc cgtcacgagc tttgccgccg
     1861 ccacggtcaa cggcgcgctc ggctacggct actcgtcgat ctcggtgccg atcgcgctgc
     1921 tggtcacggt cggccgggtg ctcaaccccg cgctggtgat cgtcgaggtc gggatcaacc
     1981 tgtacgcgct gtactggaac cggcgcaccg cgggccgcgt gatccgccgc gtgatcccgc
     2041 tggcgatcgg cctggtgccc ggcgtgatcg ccggcgcgct gctgctcggc cacatcgagc
     2101 ccaccaccat gaaggtgatc gtgtt
//
LOCUS       DASVOR010002056         2689 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_167619,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002056 DASVOR010000000
VERSION     DASVOR010002056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167619"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1578)
                     /locus_tag="VF516_38175"
     CDS             complement(<1..1578)
                     /locus_tag="VF516_38175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113640.1"
                     /translation="MRCAVAAVVVCLGLSGCAAAGAVDPDDEWGMSGPLSPTLSLGKL
                     DAELRRGLPVATDTTRTQVWTARNRWEDTDTPAARAAGLAWPADSGLTWDAKFSRWVE
                     SLAWIPSVDGDEMTVELTTPWGKTLASPMLECAETSLFLRITFAAWYELPLVLETQDD
                     HGRLVYFGHNGVRTTAGRYARSPEFAVAYRDHTGSSGWQTRWPSDPVLRGKRIAGGDD
                     DQAELGDGTHFGAYLDEIHLNKRVGYFTVMVLDYLGSANLADPANAYNLVPDAVRPGD
                     FLLERWQAVGIGHTLVVKQVDELPGGSKDVSLISGSMPRRQGIRQSGEVAKLYFTGDA
                     TGGPGANSDGVPYARLGGGLKRWRVTKAIAGYWTSTWMAGDEASWINASDLARIAARP
                     ARFEEILGQVSPEQQRTELLAQIADARQHLARYPASCSARDRREQAFAALYELSARAF
                     GQTAAEVDAASRELDDYVFAPLDYAHARTCCWDSSTPAMYDIILDAARADLAAAQTAH
                     TCAAPVVFAARADGYQRW"
     gene            complement(1789..1956)
                     /locus_tag="VF516_38180"
     CDS             complement(1789..1956)
                     /locus_tag="VF516_38180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113641.1"
                     /translation="MTDPPPFPDSLCHRCGHLRLIRSARGSSFLMCQEPSLPKYTAQP
                     VRSCPRFAPAG"
     gene            2006..>2689
                     /locus_tag="VF516_38185"
     CDS             2006..>2689
                     /locus_tag="VF516_38185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113642.1"
                     /translation="MVVLSDPAMDDLDARSEETGGEAPAGIDADLARVAAVLLEDRVV
                     RRVIKQHRQLPGIGLQVPHARCYALPRADLARLVERDELGIDPAALPDDVVIFGGSRA
                     QLAAGDPVELSRAWRAIFHARVHQALDALARTGAITAASIRERIHRIGQTEFDEIRAV
                     LRQDDLLLPPAGDTAAYVEFAALYLELRAFAPRTLGRTFPTLDAARVDAAIALDLDAA
                     ALLAASRPAT"
BASE COUNT          344 a          976 c         1020 g          349 t
ORIGIN      
        1 ccagcgctgg tagccgtcgg cgcgcgccgc gaacacgacc ggcgcggcgc aggtgtgcgc
       61 ggtctgggcg gcggcgaggt cggcgcgcgc cgcgtccagg atgatgtcgt acatcgccgg
      121 cgtcgacgag tcccagcagc aggtccgggc gtgggcgtag tcgagcggcg cgaacacgta
      181 gtcgtcgagc tcgcggctgg cggcgtcgac ctcggccgcg gtctggccga acgcccgggc
      241 ggacagctcg tagagcgcgg cgaacgcctg ctcgcggcgg tcgcgcgccg agcacgacgc
      301 cgggtagcga gcgaggtgct ggcgcgcgtc ggcgatctgc gcgagcagct cggtgcgctg
      361 ctgctcgggc gagacctggc cgaggatctc ctcgaaccgc gccggacgcg cggcgatccg
      421 cgccaggtcg ctggcgttga tccagctcgc ctcgtcgccg gccatccagg tgctggtcca
      481 gtagccggcg atggccttgg tgacccgcca gcgcttgagc ccgccgccga gccgggcgta
      541 ggggacgccg tcggagttgg cgcccgggcc gccggtggcg tcgcccgtga agtagagctt
      601 ggccacctcg ccggactggc ggatgccctg gcggcgcggc atcgagcccg agatcagcga
      661 gacgtccttg ctgccgccgg gcagctcgtc gacctgcttg accacgagcg tgtggccgat
      721 gccgaccgcc tgccagcgct cgagcaggaa gtcgccgggc cgcaccgcgt cgggcacgag
      781 gttgtaggcg ttggccgggt cggcgaggtt ggccgagccc aggtagtcga gcaccatcac
      841 ggtgaagtag ccgacccgct tgttgaggtg gatctcatcg aggtacgcgc cgaagtgcgt
      901 gccgtcgccg agctcggcct gatcgtcgtc gccgccggcg atccgcttgc cgcgcagcac
      961 aggatcgctc ggccaccgcg tctgccagcc ggacgagccg gtgtggtcgc ggtatgcgac
     1021 ggcgaactcg ggcgaccggg cgtagcggcc ggcggtggtg cgcacgccgt tgtggccgaa
     1081 gtacaccagg cggccgtggt cgtcctgggt ctccagcacc agcggcagct cgtaccaggc
     1141 ggcgaaggtg atccgcaaga acagcgaggt ctcggcgcac tcgagcatcg gcgacgccag
     1201 ggtcttgccc cacggcgtgg tcagctcgac ggtcatctcg tcgccgtcga ccgacgggat
     1261 ccaggccagc gactcgaccc agcggctgaa cttggcgtcc caggtcaggc cgctgtcggc
     1321 gggccacgcc agcccggcgg cgcgcgccgc gggcgtgtcg gtgtcctccc agcggttgcg
     1381 cgcggtccac acctgggtcc gcgtggtgtc ggtggcgacc ggcaggccgc ggcgcagctc
     1441 ggcgtcgagc ttcccgagcg acagggtcgg tgacagcggg ccagacatcc cccactcgtc
     1501 gtcgggatcg accgccccgg cggcggcgca tcccgacaga cccaggcaga caacgaccgc
     1561 ggcgactgcg catctcatgg cgtcgcgccg agcacgcttc gtgccgatcc ttcgcgatgt
     1621 cgtctcgccg ggttgcgcgc cggccggccg acgatggtcc gaccggaccg gtcgccagct
     1681 actcgactga ccaggacacc agtctccgcg cgccgccgcc acaccgccgc cggacagccg
     1741 gacagccgga cagccggaca gccggacacc ggcggccggt gccggacgtc atcccgccgg
     1801 cgcgaagcgc ggacagctgc gcaccggctg cgcggtgtac ttggggagcg acggctcctg
     1861 gcacatcagg aagctcgagc cgcgcgcgct gcggatcagc cggaggtggc cgcagcgatg
     1921 gcagaggctg tcggggaacg gcggcgggtc ggtcatggcc aggggtccag tggccccgcg
     1981 tgtgcgtggc cgggggatgc ctggaatggt agtcctatcg gacccggcga tggacgacct
     2041 cgacgcgcga agcgaggaga cggggggtga ggcccccgcc gggatcgacg ccgacctcgc
     2101 gcgcgtggcg gcggtcctcc tggaggatcg cgtggtccgc cgcgtgatca agcagcaccg
     2161 ccagctgccg gggatcggcc tccaggtgcc ccacgcgcgg tgctacgcgc tgccgcgcgc
     2221 cgatctggcg cgcctggtcg agcgcgacga gctcggcatc gacccggcgg cgctgccgga
     2281 cgacgtcgtg atcttcggcg gctcgcgcgc acagctcgcg gccggcgacc ccgtcgagct
     2341 gtcgcgggcg tggcgcgcga tcttccacgc ccgggtccac caggcgctcg acgcgctggc
     2401 gcggaccggc gcgatcaccg cggcgtcgat ccgcgagcgg atccaccgga tcggccagac
     2461 cgagttcgac gagatccgcg ccgtgctgcg ccaggacgac ctgctgctgc cgccggccgg
     2521 cgacaccgcc gcctacgtcg agttcgccgc gctgtacctc gagctccgcg cgttcgcccc
     2581 gcgcacgctc ggccggacgt tcccgaccct cgacgccgcg cgggtcgacg ccgcgatcgc
     2641 gctcgacctc gacgcggccg cgctgctcgc cgcgtcccgc ccggcgacc
//
LOCUS       DASVOR010002057         3619 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_548212,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002057 DASVOR010000000
VERSION     DASVOR010002057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548212"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..202
                     /locus_tag="VF516_38190"
     CDS             <1..202
                     /locus_tag="VF516_38190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113643.1"
                     /translation="SKPATARPSTARPPTARPATARPSTAKPPTARPPTARPAILAPS
                     VRTKTAKLARAARLRSAKEKRP"
     gene            199..612
                     /locus_tag="VF516_38195"
     CDS             199..612
                     /locus_tag="VF516_38195"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8113644.1"
                     /translation="MTPIVGVGAIVFDGDGRVLLVERGKPPSLGLWSIPGGKLEPRET
                     LAQAVAREVLEETGLVVEVGALACVLERFGDDYHFVLLDYHARVCGGQLAAASDARAA
                     RFVRFEELRDLPLTEGLADVLVRARATQPAWSTSR"
     gene            594..1874
                     /locus_tag="VF516_38200"
     CDS             594..1874
                     /locus_tag="VF516_38200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEX8113645.1"
                     /translation="MVHLPLISELISERISERISEPPVPSPGSPGSPPSLRRGDVPDA
                     QWLDWRWQLGHMLTSADELARVVALSADERAGLAASTHLFRIGITPYYASLMDPVHAS
                     CPIRLQAIPRPAEADVRAEELRDPLGEDSHNPAPSVIHKYPDRALFLVVDRCGIYCRH
                     CNRRRLVGGDEPPTTADLEAGLDYLARTPRIRDVLLSGGDPLLLSTRRLAYLLGRLRS
                     IRHIETLRIGTRLPVVCPMRIDAELTSALRAHHPLFVNTHFNHAKELTPEARAACERL
                     VDAGIPVGNQTVLLRGVNSSVRSLRALMRGLVRSRVRPYYLFQGDTVLGTDHLRTPVD
                     TAMELYRGLRGWISGMCVPELVLDAPGGGGKVPIVPSYLEHLDHERVVVRTYRGTRVD
                     YPQPRERDCTVAYDAVYFAGVPDDDDREGSAEAG"
     gene            complement(1924..2127)
                     /locus_tag="VF516_38205"
     CDS             complement(1924..2127)
                     /locus_tag="VF516_38205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113646.1"
                     /translation="MSGWTIDWFSSAAMFTGWAEYGDEPFLWRGPPTYDPLIVYQPKT
                     WLLREGWKKAGPISCRELPSVKQ"
     gene            2878..>3619
                     /gene="queG"
                     /locus_tag="VF516_38210"
     CDS             2878..>3619
                     /gene="queG"
                     /locus_tag="VF516_38210"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEX8113647.1"
                     /translation="MTAATGASMIADLGRELGFARAAVVPIEPPRRHALYRSWLASGH
                     AGTMAYLAAPEHIAQRGDLRALLATARSLIVVALAYERADPIPPDALLRGRIARYARG
                     EDYHLVMRDRLVVLADRIAAALGRPVASRPCVDSAPVLEREWAERAGLGFVAKNTMLI
                     APGLGSYVVLGELLVDAELAPTAPGEPLRPRCGACRSCLDACPTRAFVDAYVLDARRC
                     ISYLTIEHKSAIPEELRPLIGNRVYGCDD"
BASE COUNT          493 a         1432 c         1190 g          504 t
ORIGIN      
        1 atcgaagccg gcgaccgcca ggccgtcaac tgccaggccg ccgaccgcca ggccggcgac
       61 cgccaggccg tcaactgcca agccgccgac cgccaggccg ccgaccgccc ggccggcgat
      121 cctcgccccg tcggtgcgga ccaagaccgc caagctcgcc cgcgccgccc ggctgcggtc
      181 cgccaaggag aagcgcccgt gacccccatc gtcggtgtcg gtgcgatcgt gttcgacggc
      241 gacggccgcg tcctcctggt cgagcgcggc aagccgccga gcctcggcct gtggagcatc
      301 cccggcggca agctcgagcc gcgcgagacc ctggcccagg ccgtcgcgcg cgaggtcctc
      361 gaggagaccg gcctggtcgt cgaggtcggc gccctggcct gcgtgctcga gcggttcggc
      421 gacgattacc acttcgtcct gctcgactac cacgcccgcg tgtgcggcgg tcagctcgcc
      481 gccgccagcg acgcccgcgc cgcccggttc gtccgcttcg aggagctgcg cgatctgccg
      541 ctcaccgagg gcctcgccga cgtgctggtg cgcgcccgtg ctacacagcc ggcgtggtcc
      601 acctcccgct gatctccgag ctgatctccg aacggatctc cgagcggatc tccgagccgc
      661 cggtgccttc gcccggatcg cccggatcgc cgccgtcgct gcggcgcggc gacgtccccg
      721 acgcccagtg gctcgactgg cggtggcagc tcggccacat gctgacctcc gccgacgagc
      781 tcgcccgggt cgtcgcgctg tccgccgacg agcgggccgg cctcgccgcg tccacccacc
      841 tgttccgcat cgggatcaca ccctactacg cgagcctgat ggacccggtc cacgccagct
      901 gcccgatccg gctccaggcg atcccgcgcc ccgccgaggc cgacgtccgc gccgaggagc
      961 tgcgcgatcc gctcggcgag gacagccaca accccgcccc gtcggtgatc cacaagtacc
     1021 ccgaccgcgc cctgttcctc gtcgtcgacc gctgcggcat ctactgccgg cactgcaacc
     1081 gccgccggct ggtcggcggc gacgagccgc ccaccaccgc cgacctcgag gccggcctcg
     1141 actacctcgc gcgcaccccg cggatccgcg acgtcctgct gtccggcggc gacccgctgc
     1201 tgctgtcgac ccgccggctc gcctatctcc tcggccgcct ccgctcgatc cgccacatcg
     1261 agaccctccg gatcggaacc cgcctgcccg tcgtctgccc gatgcggatc gacgccgagc
     1321 tgacctctgc cctgcgcgcc caccatccgc tgttcgtcaa cacccacttc aaccacgcca
     1381 aggagctcac ccccgaggcc cgcgcggcgt gcgagcgcct ggtcgacgcc ggcatcccgg
     1441 tcggcaacca gaccgtgctc ctgcgcggcg tcaactcctc cgtgcgctcg ctgcgcgccc
     1501 tgatgcgcgg cctcgtccgc tcgcgcgtcc gcccctacta cctgttccag ggcgacaccg
     1561 tcctcggcac cgaccacctg cgcaccccgg tcgacaccgc catggagctc taccgcggcc
     1621 tgcgcggctg gatcagcggc atgtgcgtcc ccgagctcgt gctcgacgct cccggcggcg
     1681 gcggcaaggt ccccatcgtc ccgagctacc tcgagcacct cgaccacgag cgcgtcgtcg
     1741 tccggaccta ccgcggcacc cgcgtcgact acccccagcc gcgcgagcgc gactgcaccg
     1801 tcgcctacga cgcggtctac ttcgccggcg tccccgacga cgacgaccgc gagggctcgg
     1861 ccgaagccgg ataacccgac cgcgccgcgc cgcgccgccg cgccgcgcat cgccgacgag
     1921 tgctcactgc ttcaccgacg gcagctcgcg gcaggagatc ggccccgcct tcttccaacc
     1981 ttctctcagc aaccaggtct tcggctggta cacgatcagc gggtcgtagg tcggagggcc
     2041 acgccacagg aacggctcgt cgccgtactc cgcccagccg gtgaacatcg ccgcgctgga
     2101 gaaccagtcg atcgtccagc cggacatcgg cgcgcggagg tcttccccgt gctttcaaag
     2161 agtgcagcgt ggcgagccag gcgcaggcca acgatatggg gacgagatcc ggggactgcg
     2221 atccagtccg caccagcgac gaggctctgc agaacgcgtg accccgctgt cacacctgtg
     2281 gacagcgcgc ggcagccaac acggtcgacc aggcgccctg cccacaggtc tgccagcgct
     2341 gacgcccgcg cgcgggctcc atcggaggga ttgtgttcct tgtgttttca gaccggagac
     2401 tgcttccttg aaaggtccgc gcgtacctgg aaggacaagg gatgacctca tcgcgaacgt
     2461 tttgcgaatc tggtgcaacc ccgcgcgtgg actccatggg acgaacccag cccggaccat
     2521 ctcgcgaacg cagcgagcag ggctgtggct ggcgtcttct tcctcctctc ttttttccgg
     2581 acaagagaga gaaatctcct gccaggagcc tgcgcacgag cgtcgggcct gtggccggtg
     2641 agctgtcgcg ccgggtccgc catgacagcc gcgacgcgca ggtcacgggc cgagccaccc
     2701 tccacaggca gcatgtgatc tcccgaggct gcgcagtagc gccaggatcc acgaggctgt
     2761 gctcgacgat gccggctcgc cgccgatgtt gccgccctgg accgcgctgc caccgcagat
     2821 catcgtcagc gatcgaaccc acgggcgaac cgcggccgga tcgggtatga ggccgccatg
     2881 accgccgcga ccggcgcctc gatgatcgcc gacctcggcc gcgagctcgg cttcgcgcgc
     2941 gccgcggtgg tgccgatcga gccgccgcgc cggcacgcgc tgtaccggag ctggctggcg
     3001 agcggtcacg ccggcacgat ggcgtacctg gccgcgcccg agcacatcgc ccagcgcggc
     3061 gatctgcggg cgctgctggc gacggcgcgc tcgctgatcg tggtcgcgct ggcctacgag
     3121 cgcgcggatc cgatcccgcc cgatgcgctg ctgcgcggca ggatcgcccg ctacgcccgc
     3181 ggcgaggact accacctggt gatgcgcgat cggctggtcg tgctcgccga ccggatcgcg
     3241 gccgcgctcg ggcgcccggt ggcgagccgg ccctgcgtgg actcggcgcc cgtgctggag
     3301 cgcgagtggg ccgagcgcgc cgggctcggc ttcgtcgcca agaacacgat gctgatcgcg
     3361 cccgggctgg gcagctacgt ggtgctcgga gagctgctgg tcgacgccga gctcgcgccg
     3421 accgcgccgg gcgagccgct ccggccgcgc tgcggggcgt gccggtcgtg cctcgacgcc
     3481 tgcccgaccc gggcgttcgt cgacgcgtac gtgctggacg cgcggcgctg catctcgtac
     3541 ctgacgatcg agcacaagag cgcgattccg gaggagctgc gcccgctgat cggcaaccgc
     3601 gtctacggct gcgacgact
//
LOCUS       DASVOR010002058         2410 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_110395,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002058 DASVOR010000000
VERSION     DASVOR010002058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110395"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VF516_38215"
     CDS             complement(<1..293)
                     /locus_tag="VF516_38215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113648.1"
                     /translation="MGIWDKLKRAFGRGGRGGGGNDGPPSDPGTPAVASNARAGASAA
                     APAATSPARSAAEDLARYQASEILGLSMAEVRKRSLKIDPYRTPWIGRVDTIPP"
     gene            complement(579..2282)
                     /locus_tag="VF516_38220"
     CDS             complement(579..2282)
                     /locus_tag="VF516_38220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113649.1"
                     /translation="MDLRYRGRSGVVETGAGAVMTFEPNLSRSKVFFDGQIADPVRFR
                     EAISALHAVVVGDLRARKKDRSAHTAWKAARDAREKAIADAVRDRVKAAELEKIAREP
                     IPPGLEGEFRRLHHLYWTERRRWAAELAQNDPELFRHLVPCDPVVTVAPDVVMFECFA
                     KDEASYGCLSVDRDGFATAGDAGIGTTNVDYSLALFEHFQTIRSYRPTRLLVDPAGFE
                     VAVGAGASALREEKIDLPASWLRGFGQLQAAMAIPARHVELSTDVVYSLLATLRGQRE
                     KIGPRSLRFVLAPGKPVQVVIEPWGKTLTSRGRAYDGAEPAEIKVWGRRRLLAFARTL
                     PFAERFDVQLLGTGLPSVWVARLGPMRLTLALSGWTANDWSRGGSLDQYFAGFAATPG
                     TIAAVAAHLMAARSATLAQLVAVAREDDRTVLGSLHELARRGQLAYDFATGVYRWRPI
                     LDVALSESMLGPEPEEVVAGRALAQAVTLVRETEVGARRLVVGKVGGPGGGSTECEAV
                     VDADGVFASARCSCSYFYRFKLRAGPCRHLLALRLHAMRPATPVLPTATLRKLPPSRM
                     N"
BASE COUNT          353 a          900 c          850 g          307 t
ORIGIN      
        1 gggggaatcg tatcgacgcg gccgatccac ggcgtgcgat acggatcgat cttgagcgag
       61 cgcttgcgga cctccgccat cgacaggccg aggatctcgc tggcctggta gcgcgccagg
      121 tcctcggcgg ccgagcgcgc cggcgaggtg gccgcaggcg cggctgcact cgcacccgcg
      181 cgtgcattcg aggccacggc cggcgtgccg ggatccgacg gcggtccgtc gttgccgcca
      241 ccgccgcggc cgccgcggcc gaacgcgcgc ttcagcttgt cccagatccc caccggctac
      301 ggctccggcc tcgacttccg cgctccaagg agacccgatc gcccgacttc gtgcgctgga
      361 ccgcgtccga tcaccgaggg ccccgcacac ctagcagcgc accagagtct tgatctcggt
      421 ttcgtgaagc gcgctaggtg tgtcggggcc ccaaagcaca tcgggcgtgg ggatgcaatg
      481 tcagggcaat tcccgccacg gcggaacgcc gcagcatgat gggctcgtgc gctcggatct
      541 ccttgcaggg cggaagtcga tgggagggtc tggatcattc agttcatccg ggacgggggc
      601 agcttgcgca gcgtggcggt gggcaagacc ggcgtggccg gccgcatcgc atgcagccgc
      661 agcgcgagca ggtggcggca cggtcccgcc cgcagcttga accggtagaa gtacgagcac
      721 gagcaccggg cgctcgcgaa cacgccgtcg gcgtcgacca ccgcctcgca ctcggtggac
      781 ccgccgccgg ggccaccgac cttgccgacg acgagccgcc tcgccccgac ctcggtctcg
      841 cgcacgaggg tcaccgcttg cgccagcgca cgcccggcca cgacctcctc gggctcgggg
      901 cccagcatcg actcgctgag cgcgacgtcg aggatcggcc gccagcgata cacgccggtg
      961 gcgaagtcgt acgcgagctg accgcgccgg gcgagctcgt gcaggctgcc gagcacggtc
     1021 ctgtcgtcct cgcgtgccac cgcgaccagc tgcgcgagcg tggccgaccg cgccgccatc
     1081 aggtgcgcgg cgaccgcggc gatggtgccc ggcgtcgcgg cgaacccggc gaagtactga
     1141 tcgaggcttc cgccgcgcga ccagtcgttg gccgtccagc ccgacagcgc gagggtgagc
     1201 cgcatcggcc cgagccgcgc gacccacacg ctgggcaagc ccgtgccgag cagctggacg
     1261 tcgaaccgct cggcgaacgg cagcgtgcgc gcgaacgcga gcagccggcg ccggccccac
     1321 accttgatct ccgcgggctc ggcgccgtcg taggcacggc cccgcgaggt gagcgtcttg
     1381 ccccacggct cgatcacgac ctgcaccggc ttgcccggcg cgaggacgaa gcgcaggctg
     1441 cgcggcccga tcttctcgcg ctgcccgcgc agcgtggcga gcaacgagta cacgacgtcg
     1501 gtcgacagct cgacgtgccg cgccgggatc gccatggcgg cctggagctg gccgaagccg
     1561 cgcagccacg acgccggcag atcgatcttc tcctcgcgca gcgcggaggc ccccgcgccg
     1621 acggcgacct cgaagccggc gggatcgacg agcagccggg tcggccggta gctgcgaatc
     1681 gtctggaaat gctcgaacaa ggcgagcgag tagtcgacgt tggtggtgcc gatcccggcg
     1741 tcacccgcgg tcgcaaagcc gtcgcgatcc acgctgaggc agccgtagct ggcctcgtcc
     1801 ttggcgaagc actcgaacat caccacgtcg ggcgccacgg tgacgaccgg gtcacacggc
     1861 acgaggtggc gaaacagctc ggggtcgttc tgcgcgagct cggccgccca gcgccggcgc
     1921 tcggtccagt agaggtggtg caggcgacgg aactcaccct cgaggccggg cgggatcggc
     1981 tcgcgcgcga tcttctcgag ctccgcggcc ttgacgcgat cgcgcaccgc gtccgcgatc
     2041 gccttctcgc gggcgtcccg cgccgccttc cacgccgtgt gcgccgagcg atccttcttc
     2101 cgggcgcgca gatcgccgac cacgaccgcg tgcagcgcgc tgatcgcctc gcggaaccgc
     2161 accggatcgg cgatctgccc gtcgaagaac accttcgagc gcgacaggtt cggctcgaac
     2221 gtcatcaccg cgcccgcgcc ggtctcgacc acgccgctcc ggccgcggta gcgcaggtcc
     2281 atcctcacga cgacacctgc gccgacagcg tgagctcggg aagcacctgg tgcgccagct
     2341 cggccacggc gggctgcgcc cgggccacgc gggccagcgc agccagcgcc agcgcgcgct
     2401 ccgccggccg
//
LOCUS       DASVOR010002059         9519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_80357,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002059 DASVOR010000000
VERSION     DASVOR010002059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9519
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80357"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VF516_38225"
     CDS             complement(<1..386)
                     /locus_tag="VF516_38225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113650.1"
                     /translation="MREDIGQGRARGGKAATPGKRTRTEELSPTMPAPVAPGAVASGT
                     AAPGGASPDVHTAAAHGIRGGAGPLPHLEQIPRLFGPSDVGGVQAHTDPAAAEGARTM
                     GAQAAAVQRQPGAGNCDSMPFPKVQN"
     gene            691..1575
                     /locus_tag="VF516_38230"
     CDS             691..1575
                     /locus_tag="VF516_38230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113651.1"
                     /translation="MQLSLKVKQPFSFAQTVAFLRRFPPCQGDYHVTDRSITAAVSVG
                     GIARAFTLRDGDPPTVELPRHTDAATQRLLVARAGHLIGAADDLTALYAAAEGDPPFR
                     ALVEQLYGLHHVRFLTLGEIAVYCVLMQRTPVQLASTYKRRFLARFGIPVEAGGVALR
                     AMPELDELVELDAPAIAEAIGHARKAAQIAGVVRGVAAIGERFLRTAPYAAARDALLA
                     IPGIGPFSAAAILLRGLGRMDELPPIRPIEDDARWLYGAAYDAAAITRRYGAQIGYWS
                     FYLRVGAGRARQAGAAAA"
     gene            complement(1576..2802)
                     /locus_tag="VF516_38235"
     CDS             complement(1576..2802)
                     /locus_tag="VF516_38235"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX8113652.1"
                     /translation="MTAAPAVPEPLHDPPSRAIVVRLPARLTPYARTLQRWRAGCEVT
                     VLRDGGETYPAMLAALAAAQRSICFETYILAADVTGDRFKAALIERARAGVAVRVIYD
                     AVGSFGLPDAWVNELRAAGCEVIDFNPIAPWRRRFRMSHRDHRKIIVVDDRVGFTGGL
                     NIANDYASVEDGGVGWHDMHCRVTGAIVLDLARMFRRTWLRCGGTPYPPPTAAGDASA
                     AGDPPARRDTFLRLLDNTQRRQRTIVRRAYLHVIKHARERVLLQNAYFLPDRGLRRAL
                     TKAAARGVDVRVMVPGHSDVKLAQWAALYVVRRLARRGIRIFLWRGVMMHAKTATVDG
                     VWSTIGSYNFDAQSRFNNLEVTVEILDAATAQTLATNFERDIANAEPFDERAWLRLPW
                     WSKALAWISYRLRRFL"
     gene            complement(2799..3314)
                     /gene="greB"
                     /locus_tag="VF516_38240"
     CDS             complement(2799..3314)
                     /gene="greB"
                     /locus_tag="VF516_38240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEX8113653.1"
                     /translation="MSTRDRPPRPQLSRYITPEGAKKLRAELEQLWTIERPRVTQEVA
                     DAAAQGDRSENAEYIYGKRRLREIDRRLRFLQKRLDEVTVVDEAPSDPNRVFFGAHVT
                     LEDEDGEQRTYRIVGGDESDADKGWISIDSPVARACLGKRCDDTVIVRAPRGEIEYTI
                     LAVRYDAVRPA"
     gene            3334..4656
                     /locus_tag="VF516_38245"
     CDS             3334..4656
                     /locus_tag="VF516_38245"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEX8113654.1"
                     /translation="MLTAGMGSSFLIVVGLLMANGLFAGAEIAVLSVRSTRLREFIRR
                     RDRRALAVKALRDTPERFLATVQIAMTAIGTTAAAIGGARLEHELEPLFRTIGLGAGV
                     SLAAVIVLVTFLELVIGELVPKSLALRYSDRYSFFVARPLVALSQLMRPLVWLLTGVS
                     NAFLRLFGDRTTFTEARLSRDELQQLVEEAAKTGSVDPRASEIASRALGFGEVTVAEV
                     MVPRTRVVALRRGAPPEEVQRVILEQGHSRMPVYDGTIDNIVGYVVARDVLALAWERG
                     LVVLEDILRPPHQVPESTRALDLLREMQRRRTQMALVVDDHGGLSGLVTTEDLIEELV
                     GDIMSEDDVPEQFFTREPAGTILVQGWAAVRKVNRDLHLDLPVGKDRTTIAGLCMALA
                     QAIPQAGEKLVTDNGTVLEVVEASPRRVRRVRIHPVAHGDDPETLTES"
     gene            4704..5948
                     /locus_tag="VF516_38250"
     CDS             4704..5948
                     /locus_tag="VF516_38250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113655.1"
                     /translation="MTAIARDAVASDHLVYAWPFPIGPECEVSCADDEQVVMCPAWLV
                     GDRLGPGRHHWRTPDPTRPVSAFFVLTAPVEVSFDMTTMFVIPMTGQPVRLRANGSLQ
                     IRCNDPGLLIAQFVGLPFDRVNDGILRSVARSVERMLARLLTRRVVMAGTPLAVTDPG
                     MLASIVEELIAYNPTAGAVFGVELIRMGHLTIVADDGSSPYIPLPRTTEWAEVPTRKG
                     PANGDLHRNYAAAAAAQVTQRHGDISMLETSRSRPPSHGDFSARVRPIEQSSPDITPP
                     PAMIAQEVIRRAKSPQEEALPLPPPPPYGNEDITHPNVHTPAPGAMKLAAGSGQRLRD
                     EPRGAIVGIGMSPIGHSPVTAVAGEIGPRVTPGARVLVPGPNGLMQSATVRQLLHGYY
                     ELEVGHSGETIWVPVHNVVPER"
     gene            complement(5974..7263)
                     /locus_tag="VF516_38255"
     CDS             complement(5974..7263)
                     /locus_tag="VF516_38255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113656.1"
                     /translation="MVLVLVLCGACDRPCAADHLPDAPGAGPGEVRRGFVMVSNELDL
                     AATSDGVACLSCDTIFYFDGALREQRRVAAALAGSGKLAAGNAAVIGEQTYVLDFDRG
                     KDPDHGDDFYRAPNYQLFALSSSGEELWRNDLGDGEAWTGDLAPLGPIGPHRASVSIA
                     AGPGGVALHGEPLGSLFDTQGRIVWTTPTDGYDAVVPDASYGLFVAGHGPNTAAGDPQ
                     AELRRLGSEGNLVWTATWSTTHTPPIVAGGEIVFTGAARTASGGFLVAGLFSTATLDT
                     GGRILQGPNDPLPGDDFANFVAAIDNQGVTQWAYPVGGRKVNDGYVNLLQVAAVGDGA
                     VICGDYGGSNALGLPSPGTTIKAFVAHVQASGQIAAYPIQGTGELHCAGLATAPDGSA
                     IVAVQSSHDPGPGTLSIGSQTFSADAQDEFFVLNIAL"
     gene            7921..8142
                     /locus_tag="VF516_38260"
     CDS             7921..8142
                     /locus_tag="VF516_38260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113657.1"
                     /translation="MYGAAEKAGSSRGLALVRSYFQDAIGLDPLNENIRHNLEIIEKA
                     PDADAFQTPLPVEPASVLHDLQDRLQPAA"
     gene            complement(8187..>9519)
                     /locus_tag="VF516_38265"
     CDS             complement(8187..>9519)
                     /locus_tag="VF516_38265"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8113658.1"
                     /translation="PGPAAATPRVEPVPPPATLAMPGEAPLARPIVGFEIHGDSKVQP
                     RTIGYLAHVAIGDRVGPGDVAALQQALLSSELFTQVQVELQPAPGDPSPGVIVVVTAD
                     DKHSWIVAPTAFALPGNVAFGAGFAENDFRGLDQKFLIYGQVGTRNSLLFGTFLDPSY
                     HGTQLTWRTDLYLFRRNIDEYANPPDDPRSFAIERRTTATYLGGGALIGWNFLWWLMA
                     DLRLRGAYVYFRDSQDVAGQPIARLPEKNGWDITLQAHVTVDHRHHRYGVTWGTYAQL
                     DLEPSIPGLDSYGYQLMLLRAYQSWRLFGEHELELRGHFDAGRHLPLHEDLTLGGTSD
                     LRGYDVDQFRGDVRAVARAEYSVPLFKWRMFAFRALGFYDTGYAGFHTFRADRDYLLL
                     QRTASYVRNDVGAGFRIYVNNVVLPLLGLDLGYGIEGHSPEIYFEVGLTDF"
BASE COUNT         1331 a         3268 c         3538 g         1382 t
ORIGIN      
        1 tcgttctgga ccttcgggaa cggcatgctg tcgcagttgc cggcgccggg ctggcgctgc
       61 accgcggctg cctgggcgcc catcgtccgc gcgccctcgg ccgctgccgg gtcggtgtgg
      121 gcctggaccc cgccgacgtc ggacggcccg aacaaccgcg ggatctgctc caggtgcggc
      181 agcgggccgg cgcctccgcg gatcccgtgc gccgcagccg tgtggacgtc cggcgaggcc
      241 ccgcccggtg cggcggtgcc cgacgccacc gcgcccggtg ccaccggcgc cggcatcgtc
      301 ggcgaaagct cctcggttcg cgtccgcttg cccggcgtcg ccgccttgcc gccgcgggct
      361 cgaccctggc cgatatcttc acgcaacctt cgatcgaagc tcatctaccc cccacaggtt
      421 cacgatccgg acgacgttac gatgttcccg atcgcaaacg gaagcgcagc ggtcggcgcg
      481 gcatcgcatt cgtgcgtcgc gccacgctgg caaggactgc cacacgatcg cagcctcgat
      541 gcagaccgag gggcaatgct gatcgacgga gctggtcgca tccggcgctc gccgaggacc
      601 cacgggtaga tcggcccggg gatccgcggt gcgttgcctg tgcagaaaca tgacataaga
      661 atgacaattc agggccgtaa accaagttgt atgcagctct cgctgaaggt caagcagccg
      721 ttctcgttcg cccagacggt ggcgtttctg cgccgctttc cgccctgcca gggcgactac
      781 cacgtcaccg atcgctcgat caccgcggcg gtgtcggtcg gcgggatcgc ccgcgcgttc
      841 acgctgcgcg acggcgatcc gcccaccgtc gagttgccgc gccacaccga cgcggcgacc
      901 cagcgcctgc tggtggcgcg cgcgggccac ctgatcggcg ccgccgacga cctgaccgcg
      961 ctgtatgccg cggccgaggg cgatccgccg tttcgcgcgc tggtcgagca gctgtacggg
     1021 ctgcaccacg tgcggttcct gacgctcggc gagatcgcgg tgtactgcgt gctgatgcag
     1081 cgcacgccgg tccagctggc gagcacgtac aagcgccggt tcctggcccg gttcggcatc
     1141 ccggtcgagg ccggcggcgt cgcgctgcgc gcgatgccgg agctcgacga gctggtcgag
     1201 ctcgacgcgc cagcgatcgc ggaggcgatc ggacacgcgc gcaaggcggc gcagatcgca
     1261 ggggtcgtgc gcggcgtggc cgcgatcggc gagcgcttcc tgcgcacggc gccgtacgcc
     1321 gcggcgcgcg acgcgctgct ggcgatcccg ggcatcgggc cgttctcggc ggcggcgatc
     1381 ctgctgcgcg gcctgggccg gatggacgag ctgccgccga tccgcccgat cgaggacgac
     1441 gcgcgctggc tgtacggggc ggcgtacgac gccgcggcga tcacgcggcg ctacggcgcg
     1501 cagatcggct actggtcgtt ctatctccgg gtcggcgccg gccgcgcgcg gcaggcgggc
     1561 gcggcggcgg cgtgatcaca ggaaccggcg caggcggtag ctgatccagg ccagcgcctt
     1621 gctccaccac ggcaggcgga gccaggcgcg ctcgtcgaac ggttcggcgt tggcgatgtc
     1681 gcgctcgaag ttggtggcca gggtctgggc ggtggcggcg tcgaggatct cgacggtgac
     1741 ctcgaggttg ttgaaccggc tctgggcgtc gaagttgtag ctgccgatcg tcgaccacac
     1801 gccgtcgacg gtggcggtct tggcgtgcat catcacgccg cgccacagga agatccggat
     1861 gccgcggcgg gcgagccggc gcacgacgta gagcgcggcc cactgggcga gcttgacgtc
     1921 ggagtggccg gggaccatga cgcggacgtc gacgccgcgc gcggcggcct tggtcagggc
     1981 gcggcgcagg ccgcggtcgg gcaggaagta ggcgttctgg agcagcacgc gctcgcgggc
     2041 gtgcttgatg acgtggaggt aggcgcggcg cacgatcgtg cgctggcggc gctgggtgtt
     2101 gtcgagcagg cggaggaacg tgtcgcggcg ggcaggcggg tcgccggcgg cggacgcgtc
     2161 gcccgcggcg gttggcgggg ggtagggcgt gccgccgcag cgcagccagg tccggcggaa
     2221 catccgggcc aggtcgagca cgatcgcgcc ggtgacgcgg cagtgcatgt cgtgccagcc
     2281 gacgccgccg tcctcgaccg aggcgtagtc gttggcgatg ttgaggccgc cggtgaaccc
     2341 gacgcggtcg tcgaccacga tgatcttgcg gtggtcgcgg tgcgacatcc ggaaccgccg
     2401 ccgccacggg gcgatcgggt tgaagtcgat gacctcgcag ccggcggcgc gcagctcgtt
     2461 gacccaggcg tcgggcaggc cgaacgagcc gaccgcgtcg tagatcacgc ggaccgcgac
     2521 gccggcgcgc gcgcgctcga tcagcgcggc cttgaagcgg tcgccggtga catcggcggc
     2581 gaggatgtag gtctcgaagc agatcgagcg ctgggcggcg gcgagcgcgg cgagcatcgc
     2641 cgggtaggtc tcgccgccgt cgcgcagcac ggtgacctcg cagcccgcgc gccagcgctg
     2701 cagcgtgcgg gcgtacggcg tcagccgggc cggcagccgg acgacgatcg cgcgcgatgg
     2761 cggatcgtgg agcggctcgg ggacggcggg cgcagcggtc atgcgggccg aacagcgtcg
     2821 tagcgtacgg cgaggatggt gtactcgatc tccccgcgcg gcgcgcggac gatgacggtg
     2881 tcgtcgcagc gtttgccgag acatgcccgc gcgaccggtg agtcgatgct gatccagccc
     2941 ttgtcggcgt cggattcatc accgccgacg atgcggtagg ttcgctgctc accgtcctcg
     3001 tcctcgagcg tgacgtgggc gccgaagaac acgcggttcg gatcgctggg cgcctcgtcg
     3061 accacggtga cctcgtcgag ccgcttctgc aggaaccgga gccggcggtc gatctcacgc
     3121 agccggcgct tgccgtagat gtactcggcg ttctcgctgc ggtcgccctg tgcggcggcg
     3181 tcggcaactt cctgagtgac ccgtgggcgc tcgatcgtcc acagctgctc gagctcggcg
     3241 cgcagcttct tcgcaccctc gggcgtgatg tagcgcgaca gctgaggccg cggcggtcgg
     3301 tcccgcgtcg acattcgccg cagcatatcc ggtatgctga ccgcgggcat gggctcgtcg
     3361 ttcttgatcg tggtcgggct gctgatggcc aacgggctgt tcgcgggggc cgagatcgca
     3421 gtgctctcgg tccggagcac gcggctccgc gagttcatcc ggcgccgcga ccgacgcgcg
     3481 ctcgcggtca aggcgctgcg cgacaccccc gagcggttcc tcgcgaccgt gcagatcgcg
     3541 atgacggcga tcggcaccac ggccgccgcg atcggcggcg cccggctcga gcacgagctg
     3601 gagccgctgt tccgcaccat cggcctgggc gccggggtct cgctcgccgc ggtgatcgtg
     3661 ctggtgacgt tcctcgagct ggtgatcggc gagctggtgc ccaagtcgct cgcgctgcgc
     3721 tactccgacc gctactcgtt cttcgtcgcc cggccgctgg tcgcgctgtc gcagctgatg
     3781 cggccgctgg tgtggctgtt gaccggggtg tccaacgcgt tcctccggct gttcggcgac
     3841 cggaccacgt tcaccgaggc gcggctgtcc agggacgagc tgcagcagct ggtcgaggag
     3901 gccgccaaga ccggctcggt cgacccgcgg gcgagcgaga tcgcgtcgcg ggcgctcggc
     3961 ttcggcgagg tcaccgtcgc cgaggtcatg gtgccgcgga cccgggtcgt ggcgctgcgc
     4021 cggggcgcgc cgcccgagga ggtccagcgc gtgatcctcg agcagggcca cagccggatg
     4081 ccggtctacg acggcacgat cgacaacatc gtcggctacg tcgtggcgcg cgacgtgctc
     4141 gccctggcct gggagcgcgg cctggtggtg ctcgaggaca tcctgcgccc gccgcaccag
     4201 gtcccggagt cgacccgcgc gctcgacctg ctgcgggaga tgcagcgccg gcgcacccag
     4261 atggccctcg tggtcgatga ccacgggggg ctgtccggcc tggtgacgac cgaggacctg
     4321 atcgaggagc tggtgggcga catcatgagc gaggatgacg tcccggaaca gttcttcaca
     4381 cgcgaacctg ctggaacgat tcttgttcag ggctgggccg cggtgcgcaa ggtcaaccgc
     4441 gatcttcacc tggatctgcc ggtcggcaag gaccggacga ccatcgcggg gctgtgtatg
     4501 gccctcgccc aggcgatccc ccaggccggg gagaagctcg tcaccgacaa cgggacggtc
     4561 ctggaggtgg tcgaggcctc cccccgccgg gttcggcgcg taagaatcca tcccgtcgcc
     4621 cacggcgacg atcccgaaac acttactgaa tcctgatacg ccagggctcg ccgaatcctt
     4681 cccgtcttgt actatcagcg tcgatgacgg ccatcgcgcg cgacgccgtc gcgtccgacc
     4741 atctcgtcta cgcctggccg ttcccgatcg gtccggagtg cgaggtgtcg tgcgcggacg
     4801 acgagcaggt cgtgatgtgc ccggcgtggc tggtcggcga ccggctcggc ccgggccggc
     4861 accactggcg cacgcccgat ccgacgcgcc cggtgtcggc gttcttcgtg ctgaccgcgc
     4921 ccgtcgaggt gtcgttcgac atgacgacga tgttcgtcat cccgatgacc gggcagccgg
     4981 tgcggctgcg ggcgaacggc tcgctccaga tccgctgcaa cgacccgggg ctgctgatcg
     5041 cccagttcgt cggcctgccg ttcgaccggg tcaacgacgg catcctgcgc agcgtggctc
     5101 gcagcgtcga gcgcatgctc gcgcggctgc tcacccggcg cgtcgtgatg gccgggacac
     5161 cgctcgcggt gaccgatccc ggcatgctgg ccagcatcgt ggaggagctg atcgcctaca
     5221 accccaccgc gggcgcggtg ttcggcgtcg agctgatccg tatgggccac ctcacgatcg
     5281 tcgccgacga cggctccagc ccgtacatcc cgctgccgcg gacgaccgag tgggccgagg
     5341 tgcccacccg caaggggccg gccaacggcg acctgcaccg caactacgcc gcggcggccg
     5401 ccgcgcaggt cacgcagcgc cacggcgaca tctcgatgct cgagacctcg cgtagccggc
     5461 cgccgagcca cggggacttc tcggcgcggg tccggccgat cgagcagtcg tcgccggaca
     5521 tcacgccgcc gccggcgatg atcgcccagg aggtgatccg gcgcgccaag agcccgcagg
     5581 aggaggcgtt gccgctgccg ccgccgccgc cgtacggcaa cgaggacatc acccacccca
     5641 acgtccacac gccggcgccg ggggcgatga agctcgccgc cggatcgggc cagcggctgc
     5701 gcgacgagcc gcgcggcgcg atcgtcggca tcggcatgtc gccgatcggg cacagcccgg
     5761 tcacggccgt cgcgggggag atcggccccc gggtcacgcc cggcgcccgc gtcctcgtgc
     5821 ccggtcccaa cggcctgatg cagtcggcga ccgtccgcca gctgctccac ggctactacg
     5881 agctcgaggt cggccactcc ggcgagacga tctgggtccc ggtccacaac gtcgtgcccg
     5941 agcgctgagc cgggcgcgcc gcggccgcgc tcgtcacagc gcgatgttca ggacgaagaa
     6001 ctcgtcctgc gcgtcggcgc tgaacgtctg gcttccgatg ctgagcgtgc cggggcccgg
     6061 gtcgtgagag ctctgcaccg cgacgatcgc gctgccgtcc ggggcggtgg cgaggccggc
     6121 gcagtgcagc tcgccggtcc cctggatcgg atacgccgcg atctgcccgc tcgcctggac
     6181 atgtgccacg aatgccttga tggtcgtgcc ggggctgggc aagccgagcg cattgctgcc
     6241 gccgtagtcg ccgcagatca ccgcgccgtc gccgaccgcg gccacctgca gcagattgac
     6301 atagccgtcg ttgaccttcc ggccgccgac cgggtaggcc cactgcgtga cgccctggtt
     6361 gtcgatggcg gcgacgaagt tggcgaagtc gtccccgggg agcggatcgt tcggaccttg
     6421 caggatccgg ccgccggtat cgagggtcgc ggtgctgaac aaccccgcga ccaggaagcc
     6481 gccgctcgcc gtgcgcgcgg cgccggtgaa gacgatctcg cctccggcga cgatcggcgg
     6541 cgtgtgggtg gtgctccacg tcgccgtcca caccaggttg ccctcgctgc cgagacggcg
     6601 gagctcggcc tgcgggtcgc cggccgcggt gttcggcccg tggccggcga cgaacaggcc
     6661 ataggacgcg tcggggacga cggcgtcgta gccgtcggtg ggggtcgtcc acacgatccg
     6721 gccctgggtg tcgaacagcg agcccagcgg ctcgccgtgg agggcgacgc caccgggacc
     6781 cgcggcgatc gacacggagg cgcggtgggg gccgatgggg cccagcgggg cgaggtcgcc
     6841 ggtccacgcc tcgccgtcgc cgaggtcgtt gcgccacagc tcctcgccgg agctcgacag
     6901 cgcgaacagc tggtagttcg gcgcgcgata gaagtcgtca ccgtgatcgg ggtccttgcc
     6961 gcggtcgaag tcgaggacgt aggtctgttc gccgatgacc gcggcgttgc cggcggcgag
     7021 cttgccggac cctgccaggg ccgccgcgac gcggcgctgc tcgcgcagcg cgccatcgaa
     7081 gtagaagatc gtgtcgcagc tcaggcaggc cacaccgtcc gacgtcgccg cgaggtcgag
     7141 ctcattgctg accatgacga agccgcggcg aacctcaccg gggccggcgc ccggcgcatc
     7201 cggcagatga tcggccgcgc acgggcgatc gcacgcgcca cacaggacca gaacgaggac
     7261 cacgaacgat cgcatgggcg gtcgcactgc aggccgcaga ccaggtgctc cgccagggtt
     7321 ctcgcgccga tagccgcgat ggccggccgc gccctgacat cgctgtcagg tcagaagtcg
     7381 gtcaggccga cctcgaagta gacctcgggg gagtggccct cgatgcgggc ccaacggcct
     7441 gatgcagtca gccaccgtcc gccagctgct ccacggctac tacgagctcg aggtcggcca
     7501 ctccggcgag acgatctggg tgccggtcca caacgtcgtc cccgagcact gagcggacat
     7561 cgagcgccgg ggtagcgcgt gaggtggtag gtcctggccg agaggatctc ggtaccgctg
     7621 ggagcggcgt cctcggagcg gcgacggcgc cctggcgttt gcccagatgc tacgctcccg
     7681 ccgtgccgaa ggtgtatcgt gcgatgacgc cagcgagtag cgggctaccg caaatcgcgg
     7741 cgactgcgcg ctcgctcggg gtcagggtgc cgaccgacat cgcgcccgat gaggccggcg
     7801 atgtccaccc gggcccacga gggatgtcgg tggcgccgga tctcgtcagc ttgccgttgc
     7861 accggatccc caagcgcctg gcgcacttga gattgggagc gatcggcccg gacgccgacg
     7921 ttgtacggag ccgcggagaa ggccggttcc tcgcggggcc tcgcgctggt ccgctcgtac
     7981 ttccaggatg cgatcgggct cgatccgctc aacgagaaca tccggcataa cctggagatc
     8041 atcgagaagg ctccggacgc cgatgcgttc cagaccccgc tgccggtgga gcctgcatcg
     8101 gtgctccacg acctccagga tcggctccag ccagcagcat gacaccagct ggtcccaagc
     8161 cggcgctctg agcggtacac cggcggtcag aagtcggtca ggccgacctc gaagtagatc
     8221 tcgggggagt ggccctcgat gccgtagccg agatcgaggc cgagcagcgg cagcacgacg
     8281 ttgttgacgt agatccggaa gccggcgccg acgtcgttgc ggacgtagct cgcggtgcgc
     8341 tggaggagca ggtagtcgcg gtcggcgcgg aaggtgtgaa agccggcgta gccggtgtcg
     8401 tagaagccca gcgcgcggaa cgcgaacatc cgccacttga acagcgggac cgagtactcg
     8461 gcgcgggcca ccgcccggac gtcgccgcgg aactggtcga cgtcgtagcc gcgcagatcc
     8521 gaggtgccgc cgagcgtcag gtcctcgtgc agcggcaggt ggcggccggc gtcgaagtgg
     8581 ccgcgcagct cgagctcgtg ctcgccgaac agccgccagc tctggtaggc gcgcagcagc
     8641 atcagctggt agccgtagct gtcgagcccg gggatcgagg gctcgaggtc gagctgggcg
     8701 taggtacccc aggtcacgcc gtagcggtgg tggcggtggt cgacggtcac gtgcgcctgg
     8761 agcgtgatgt cccagccgtt cttctcgggg agcctggcga tcggctggcc ggccacgtcc
     8821 tggctgtcgc gaaagtacac gtaggcgccg cgcagccgca ggtcggccat cagccaccac
     8881 aggaagttcc agccgatcag cgcgccgccg cccaggtagg tcgcggtcgt gcggcgctcg
     8941 atcgcgaacg accgggggtc gtcgggcggg ttggcgtact cgtcgatgtt gcggcggaac
     9001 aggtagaggt cggtgcgcca ggtcagctgg gtgccgtggt acgacgggtc gaggaacgtg
     9061 ccgaacagca gcgagttccg cgtgccgacc tggccgtaga tcaggaactt ctggtcgagg
     9121 ccgcggaagt cgttctcggc gaagccggcg ccgaacgcga cgttgccggg cagcgcgaac
     9181 gcggtcggcg ccacgatcca cgagtgcttg tcgtcggcgg tgaccacgac gatcacgccg
     9241 gggctcgggt cgccgggggc cggctgcagc tcgacctgga cctgggtgaa cagctcgctc
     9301 gacaggaggg cctgctgcag cgccgcgacg tcgccggggc cgacccggtc gccgatcgcg
     9361 acatgggcga ggtagccgat cgtgcgcggc tggaccttgg agtcgccgtg gatctcgaag
     9421 ccgacgatcg ggcgggcgag cggcgcctcg ccgggcatcg cgagcgtcgc cggaggcggg
     9481 accggctcga cgcgcggcgt cgcagcggcg ggccctggc
//
LOCUS       DASVOR010002060         4507 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_80361,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002060 DASVOR010000000
VERSION     DASVOR010002060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80361"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            435..776
                     /locus_tag="VF516_38270"
     CDS             435..776
                     /locus_tag="VF516_38270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113659.1"
                     /translation="MKTDTFDTLTADQLDRPYGGIPAAAQWVMMHESGGSTTAGHLHA
                     QGRGDGTPGNHSSAFGAFQMIEAQRKHYMGADYQSTDFNKQYAAATHYVTDRYGSWGA
                     AKSFWQAHRWY"
     gene            complement(807..1064)
                     /locus_tag="VF516_38275"
     CDS             complement(807..1064)
                     /locus_tag="VF516_38275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113660.1"
                     /translation="MSALLVAALMPLAACAAPSTWGYASAPVAARTEPTTWGYATLQT
                     GEPAPAVAGFSKQPAPAAGPTVWGFAKLPAPEPAADVTARR"
     gene            1576..1803
                     /locus_tag="VF516_38280"
     CDS             1576..1803
                     /locus_tag="VF516_38280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113661.1"
                     /translation="MKTHRVFTILVALVLVPFLLSWGTLLLIPAALLLVPCVLLAAIA
                     AVPALLVAAASTRHDTAHPQTPVSTTPIYST"
     gene            1852..2493
                     /locus_tag="VF516_38285"
     CDS             1852..2493
                     /locus_tag="VF516_38285"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HEX8113662.1"
                     /translation="MYLPAQFHETRTPVLHDLIRAHPFGVLVTLDATGLVANHLPMEL
                     DATAGELGTLRGHVARANPVWKTHRPDVEAMAVFQGPQCYITPSYYATKAATGKVVPT
                     WNYATVHAHGPLRVIEDAAWLRRLVEQLTDRHEATRPAGEAPWHVGDAPEAFIETMIK
                     AIVGIELPIARLEGKWKVSQNRPAEDRAGVVAGLGAGDPMQRAMAELVRERMA"
     gene            complement(2516..2791)
                     /locus_tag="VF516_38290"
     CDS             complement(2516..2791)
                     /locus_tag="VF516_38290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113663.1"
                     /translation="MWFAWGMTLELAELVRGERGHDRRALQMAGVAIGCPANFAWRGH
                     RRTRPEYQRDADTAALLHRRGLAWAMDLTAATREVHALYRIREWGED"
     gene            complement(3259..3510)
                     /locus_tag="VF516_38295"
     CDS             complement(3259..3510)
                     /locus_tag="VF516_38295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113664.1"
                     /translation="MNRIIFIGTDGGGWYIGKDGKIHRFPGWNPEELKDVAHMLAALR
                     DVSQIKTAGVAERLSASLHETVTKQLGAHIQAGDVVVLG"
     gene            4069..4482
                     /locus_tag="VF516_38300"
     CDS             4069..4482
                     /locus_tag="VF516_38300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113665.1"
                     /translation="MKRIKLLGLAAMMLVPIGVVYAGAHNSVEVSITLDDTGTVVLGA
                     FGNEADAFNSVDSNQYIGCGGNTVSGFCQAHDATADADGVLNSAFCFTQNTQLLEQIR
                     SVAADSFVSFNMDPTTGECLNIRVSHNSFYRPKAP"
BASE COUNT          680 a         1587 c         1573 g          667 t
ORIGIN      
        1 cggcaagctc gcgatcgtcg acttcacccg cgaggccacg cacgggccgc cgccggagat
       61 gcggctcacc cccgagcagg tgatcggcga gctgcgccag gccgggctcg cggcggcgct
      121 gtcgccgacc gtgctgcccg accagtacat cgtggtcggc acccgctgaa caggtcagtc
      181 gagtagccgc gccgccggcc actcgacctg ccggtcatca gcgctgcgat cggggcggcg
      241 atcgaggctg cgatcgtgcg gcagctcgcg caatcgcgcg cgccgcgagc gcgatgacag
      301 atcgcgttca cgcgaagtgc acggcgctgt cgaccgcggg agacgaggtg attcccgagg
      361 aagacagcgg atctctggca cctgggctgc acttgcgacg gcgcatcttt ccaccagcca
      421 cccgaggagc cacgatgaag accgacacgt tcgacacgct caccgccgac cagctcgacc
      481 gcccgtacgg cggcatcccc gccgccgcgc agtgggtcat gatgcacgag tccggcggca
      541 gcacgaccgc gggccacctc cacgcgcagg gccggggcga cggcacgccg ggcaaccact
      601 cgtcggcgtt cggcgcgttc cagatgatcg aggcccagcg caagcactac atgggcgccg
      661 actaccagtc gacggacttc aacaagcagt acgccgcggc gacccactac gtcaccgatc
      721 gctacgggtc gtggggcgcg gcgaagagct tctggcaggc gcaccgctgg tactgagccg
      781 ccgcagggcg gtattgccgg cggcagctag cgccgcgcgg tgacgtccgc ggcgggctcc
      841 ggcgcgggca gcttcgcgaa gccccagacc gtcgggccgg ccgccggcgc gggctgcttc
      901 gagaacccgg cgaccgccgg ggcgggctcg ccggtctgca gcgtggcgta gccccacgtc
      961 gttggctcgg tgcgtgcggc caccggcgcg ctggcgtagc cccaggtcga aggcgcagcg
     1021 cacgcggcga gcggcatcag ggccgcgacc aggagcgcgg acaacgaggc ggacagtgag
     1081 gcggacagtg aggtcgaggt atgcgagctg gatcgtttcg acatggcgat tctcccgaat
     1141 gccggtgcag caactcccgt gcaagggagg cgccgtggca aaggctcgta cgtacgtctc
     1201 gtagtccggt tactcgcgat cgatcgcgat ggcggcctgc aagacgtgcg cggagcctcc
     1261 gcgcggccag cgtgaggtgt cgtcctgcgg aggagatcac gctcggcgaa gatcgcgcaa
     1321 gagatgcagc caccacgagc tgccggcggc acagcgacga gttcgtcgga cccatggagt
     1381 gcggcgtacc cgggtactcc gcatccattg caggttcttg ggagccccgc gccgcggcgc
     1441 cgacgggaga atgtcggtaa cgatcggatg ccgatccgca cggcacgctg caagtccgca
     1501 tggataggac cgtgctcgtc gatctcgcag cggcatttgt gacactggcc gaccgtttgc
     1561 tgtgtacgtg atcgcatgaa gacccaccgc gtgttcacga tcctggtggc cctggtgctg
     1621 gtgccgttcc tgctgtcctg gggcacgctc cttctgatcc cggccgcgct gctgctggtg
     1681 ccgtgcgtcc tgctcgccgc gatcgccgcg gtgccggcgc tgctggtcgc agcggcgtcg
     1741 acgcggcatg acacggcgca cccgcagacg ccggtgtcca ccacgccgat ctactcgacc
     1801 tagccccggc aatcccaacg cgcagatcgt gcgcgtggca cgatagcgcc catgtatctc
     1861 ccggcgcagt tccacgagac gcggacgcct gtcctccacg atctgatccg cgcccatcca
     1921 ttcggcgtgc tcgtcaccct ggatgcgacc ggcctggtcg cgaaccacct cccgatggag
     1981 ctcgacgcga ccgccggcga gctcggcacg ctgcgcggcc acgtcgcccg cgccaacccg
     2041 gtgtggaaga cgcaccggcc cgacgtcgag gcgatggccg tgttccaggg cccgcagtgc
     2101 tacatcacgc cgtcgtacta cgcgaccaag gcggcgaccg ggaaggtcgt gccgacctgg
     2161 aactacgcca cggtccacgc ccacggcccg ctgcgggtga tcgaggacgc ggcctggctg
     2221 cgccggctgg tcgagcagct caccgaccgc cacgaggcga cgcggccggc cggcgaggcg
     2281 ccgtggcacg tcggcgacgc gcccgaggcg ttcatcgaga cgatgatcaa ggcgatcgtc
     2341 ggcatcgagc tgccgatcgc gcggctcgag ggcaagtgga aggtgagcca gaaccgcccg
     2401 gccgaggatc gcgccggcgt ggtggcgggc ctcggcgccg gcgatccgat gcagcgcgcg
     2461 atggccgagc tggtccgcga gcggatggcg tgaccgctgc ggcccgcgcg ccgcgtcagt
     2521 cttctcccca ctcgcggatc cggtacagcg cgtggacctc gcgggtcgcc gcggtgaggt
     2581 ccatggccca ggccaggccg cggcggtgca gcagcgccgc ggtgtcggcg tcgcgctggt
     2641 actccggtcg ggtccggcga tggccgcgcc aggcgaagtt cgccgggcag ccgatcgcga
     2701 cgcccgccat ctgcagcgcg cgccggtcgt ggccgcgctc gccgcgcacg agctccgcga
     2761 gctcgagcgt catgccccat gcgaaccaca tcaggtcgcc ctcgagcgtg tggcgaccgg
     2821 cggtggcgct gcgcggatcg tccggtgcga tgcgcgccgc gcgctccggc gccgccggta
     2881 ggtcgtcgat cgcgaaccgg tggatcgcgg tcaggtaatc ggcctggacc tcggaccgcc
     2941 gcggatcccc gaagtgccgg tcgaagatcg cggcgaccag ctcgtcgacc tcggtgatcg
     3001 ccgcggcgac ctcgggccgg gcgagctcgt cgtggatccg ctgctgaccg gactcgtaca
     3061 gcgccgcctg ggtccatgcg cggaacagcg gcggccgggt cccgcgcggc agcagcagcg
     3121 tgagcaggcc cggctcgccg cgatcgtcgc cgaagtagaa tcgcccgcgc gactccggat
     3181 gatagacgtc ccagtactcg gtccatgcat cgagctcatg gaagtacgag acgtggcggg
     3241 cgaggtcgtc gggtgcgctc agccgagcac caccacgtcg ccggcctgga tgtgggcgcc
     3301 gagctgcttg gtcacggtct cgtgcagcga tgccgacagc cgctccgcga cgccggcggt
     3361 cttgatctgc gaaacatcgc gcagcgccgc gagcatgtgg gcgacgtcct tgagctcctc
     3421 cggattccag cccggaaagc gatggatctt gccgtccttg ccgatgtacc agccgccgcc
     3481 gtccgtgcca atgaagatga tccggttcat tgcttgtctc ctccttgcgc ccgtacccga
     3541 cgagcctgat cgcggcgaga ggcgcgtggg tggtcgtcgc gatgcctccg ctccctgcgg
     3601 ggctgcctcg tgttcgtacg atacgccgcc gcatggcgac gcacaatcga ttcctcatga
     3661 ggattgccgg atcgcctgcg cacgcgcgcg gctgcgattg ccagacgcgc agtgcttgcg
     3721 ctcgcgcgca aacgcatcgc cgatcaccgc cgttgcaagc ggcgatcgtt gccagggcgt
     3781 gtgaaggcaa gacgagcgct gacccaccgg aaggtgatcc ggcgggcagc gcgacctgac
     3841 acgtgacgac accatgtcga cacgcgcgtc cgcggcgctg ctcgcgtgaa gctcctccag
     3901 cccggtatcg tcgagtcatc gcctcgtggt ctcgccggct tgcgcggatc tcgcgccgat
     3961 cgatcacacg cggcggccgg cgcgcgtccg gagatcgtcc cgcggatcgg gaggtctttc
     4021 catcgatgga tggcgcacct tctgcaacgc cggcgcgcgg aggttttgat gaagcgaatc
     4081 aagctactcg gtcttgcggc gatgatgctc gttccgatcg gcgtcgtgta cgccggcgcc
     4141 cacaactcgg tcgaggtctc gatcacgctc gatgacaccg gcacggtcgt cctcggggcg
     4201 ttcggcaacg aagccgacgc gttcaacagc gtcgactcca accagtacat cggctgcggc
     4261 ggcaacacgg tcagcgggtt ttgccaggcg cacgatgcca ccgccgacgc cgacggcgtc
     4321 ctcaattcgg cgttctgctt cacccagaac acgcagctgc tcgagcagat ccggtcggtc
     4381 gccgccgact cgttcgtgtc cttcaacatg gacccgacca ccggcgagtg cctgaacatc
     4441 cgggtgtcgc acaactcgtt ctaccggccg aaggcgccgt gaccgcgatg cgacgcatcc
     4501 tgctctc
//
LOCUS       DASVOR010002061        11324 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_430272,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002061 DASVOR010000000
VERSION     DASVOR010002061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430272"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VF516_38305"
     CDS             <1..232
                     /locus_tag="VF516_38305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113666.1"
                     /translation="PSSLLHRAAPSAAPCWWFRVDVDCAGIAAGRSVVDMPPDDSNPR
                     ARRGARDLAGLRASIVRLPDSRPKSGRTPDNA"
     gene            717..1901
                     /locus_tag="VF516_38310"
     CDS             717..1901
                     /locus_tag="VF516_38310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HEX8113667.1"
                     /translation="MIRAPRSLAPPGVINVEDLRRRAKRRLPRVVFNYVDGGADDEVT
                     LRENCSAFQGVTFRPRQAVVVPSHDLRTRVLGCELAFPALLAPIGYLRLIHPAGELAA
                     ARAAGAAGTAFVQSTISGHALERTRAASTGPVGYQLYLVGGRAASEAAIERARQAGFS
                     ALFVTIDTPVAGLRERDLRGGMKELLSGSLAAKLRFLPQFFTRPRWVAGFLRDGGLPR
                     LVNVVPPGEPPMPLVDVVGALARSGVTWEDFRWIRQAWTGPLVAKGVLTGDDARRAID
                     HGATAIVVSNHGGRQLDSASATLRALPEVVAAVNGQAEVLLDGGIRRGSDIVKAICLG
                     ARAVLVGRAYVYGLAAAGEAGVARALEILRADVERTLRLIGCPSIAALDASYVEARPG
                     WR"
     gene            complement(1949..3016)
                     /locus_tag="VF516_38315"
     CDS             complement(1949..3016)
                     /locus_tag="VF516_38315"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8113668.1"
                     /translation="MSSQVSRDIARLRRIEHDESERFYAELMGAPGTTSDADEAERFY
                     AELVGRPSARRSPSRWFEDESSTTPAAGACIRPDRELRFASGVTAPAVLSFPHTAICK
                     ILVLKRAGSLAGIGIVDEYGRPVEQPPDRPSDDPSGSAVAIDPSLTGLVQGEGTGFYV
                     DHNRIVTAAHVVLGAIDVVVIPAKNGPGIAPPGRTHHDEPFGRLRVAGSAVHVFPDLL
                     RVAALSSTSPDVFFAEDLAVLHPVPPPHGYPMRPGILGRFFPVPPPAANECVNTSGYS
                     AELVDLNFQHTDVGMCLRRRGKLVDYDLMSEKGASGSPIWKGPLLPSGTPACGIVLGG
                     NTRLDTFSLVFTPAKQRFMFR"
     gene            complement(3666..3941)
                     /locus_tag="VF516_38320"
     CDS             complement(3666..3941)
                     /locus_tag="VF516_38320"
                     /inference="COORDINATES: protein motif:HMM:NF039716.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6289 family protein"
                     /protein_id="HEX8113669.1"
                     /translation="MNFLEWIVESEKGYIMFRRNALFALALAIGTLAVSAAPARAIPT
                     GNTLTVIAYYSDPGKTQLVGQKWSGCGQPSGSWGTTTPFSNLFFPAC"
     gene            complement(4343..5092)
                     /locus_tag="VF516_38325"
     CDS             complement(4343..5092)
                     /locus_tag="VF516_38325"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEX8113670.1"
                     /translation="MTARATTIALEGRVALVTGGSRGIGRAAALGLAESGADVAVNYR
                     TRGEEAAAVVRQVEALGRRAIAVGADVSREPDVERLFAEVTAALGPIDVLVSNAGIAN
                     PATIDTLDLARWDEAMAVNLRSAFLVSSKAIASMRARRWGRIIYVTSTAAQTGGIVGP
                     HYAASKAGLIGLMHGYASWLARDGVTVNAIAPALIETDMTSASAKARADRIPVGRLGR
                     VEEVAGLVCEIAANGYITGQTFQVNGGLYMT"
     gene            5201..5434
                     /locus_tag="VF516_38330"
     CDS             5201..5434
                     /locus_tag="VF516_38330"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEX8113671.1"
                     /translation="MSIEHGLACGRFGVADLPMPMVDVVGALARSGVTWEDFRWIRQA
                     WTGPLVAKGVLTGDDARRAIDHGVTAGVSRSSE"
     gene            complement(5480..6040)
                     /locus_tag="VF516_38335"
     CDS             complement(5480..6040)
                     /locus_tag="VF516_38335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113672.1"
                     /translation="MDSTDMSTPVTRGELQDALGLFRRELKQELALERQQELQAAITP
                     LATKAELQQEIQAAIAPLATKAELQQEIQAAIAPLATKAQLELWGGALVARIQQGEHR
                     IGNLERGQEQLIGLVQQTQQQIQQTEQRLLAELARHTSAIHEAMRAEIKASFEPLADL
                     PGRMSRVEAAVFGPRPPDRPTRRRKH"
     gene            complement(6707..7264)
                     /locus_tag="VF516_38340"
     CDS             complement(6707..7264)
                     /locus_tag="VF516_38340"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEX8113673.1"
                     /translation="MLTADVRRANQTTNTPLAPRQQLRRGDEVALYVDLDHDAYVYVM
                     YVDAKGEISELFPAAGEVIVPRGRQQLPPGKRWRLDAAMGFESFIVLAAATPLDRAVR
                     LTRAEQSRPAPDTTRERTARSEPRVPPQPRDSSRKPAQLTPPRDPETSHPQEDTRRPT
                     EVDDRVVAKPDAHGVTTAVLTIDHR"
     gene            complement(7408..8991)
                     /locus_tag="VF516_38345"
     CDS             complement(7408..8991)
                     /locus_tag="VF516_38345"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8113674.1"
                     /translation="MKHVILFVAANPAGTDRLALDREARAIQHELERARSGDRFEFIS
                     RWAAEPLDLLRELRKLRPTVVHFSGHGERVANEPQRDVQARRDVSPVMGSDEPNGLCF
                     QGPDGGAVMVSPEALCKTFRSAGPSVKLVVLNACYTASQAEALVGHVDCVLGMNGSIQ
                     DVAARSFAIGFYGGLGERESIAVAYQQGCAAIGLEGLRDDEHPQLVVRGGSDASQLVL
                     AVMRPAGVGATSRVAAGLAIATAILAWLVFRVKPPPADMIGFPAVKFEMGSTPQEIQT
                     AYQDCQSHRYRGCRRELFEREGPVRAVALSPFAIDRTEVTNQAFARWLSSASTVSDGE
                     LVRMNGSLLLNLDASELQRGNRSFEPRPGTERHPVIGVAPAGAQAYCAALDKRLPTEA
                     EWEYAARGIERRRYPWGNSPEPEQLGCALQIGKVPGTNCPDPRPLADVASVAADVTPN
                     GVHDLAGNASEWVADRFSDGYPDCGECRDPQVTEGENQVFRGGNRELDASVVRAAARS
                     RAQARFAAYHTGFRCALSR"
     gene            9369..>11324
                     /locus_tag="VF516_38350"
     CDS             9369..>11324
                     /locus_tag="VF516_38350"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113675.1"
                     /translation="MGAAQRALNRFTGGKLDAEGVKMIREVGPIAEHAIAAISRAVVE
                     VRVHNVLEEASRLVAAATQSFYTDECTTAAESAGTGVGPASLSDVVGFEREAGEAFQS
                     ANYEKAASLMQSVYETQKGLLGAGHPVVLHLLNKRAAVQLAVNNYKQATAFGLTALQR
                     RMQSLQGIKVTFPPSHEEVAAALAVAESASTLASIYSASGVYAEAKQNYEHAIELRSH
                     YLGVDHQCAAQSQNGLGELLQLMGDYPAARRLFESALRTRETKLGPRHRDTARSINDL
                     GSLHRSIGDLLHAKEFYRRALDVRLTFGDDDVDVAESHNNLGDLYKLRGEYANAESEY
                     RQAFDIRRKKFGEGVEAAQSQIAIANLYQMMGDYRRAQDLYNIVCEIRQKVLGPDHPD
                     TAESLSALGDSHRAQRDYTRAESEYRQALEIRRNKLGPDHPLVASSLVALGNVHLDLG
                     DYAKAESEYNQARDIQQNKLGAEHPDVAQSQHQLGLLHQARGDYAEAESLLRSALSLR
                     DTKLGAGHPSVAESLVALAKLMVATGRHPQALAYVHRAFGISEQLLQSVGAVASERVD
                     ALLFGGAFGLIHSGRWRGSTQVAAARRDRDPPGPDAGIRIRLDPFRWIGWIDWIRRRR
                     RLAASIWRGLEEAPDGRGVVVLEVVLGD"
BASE COUNT         1750 a         3819 c         3866 g         1889 t
ORIGIN      
        1 gccgtcgtcg ctgctgcacc gagctgcccc gagtgcggct ccgtgctggt ggttcagagt
       61 ggatgtcgat tgtgccggca ttgcggctgg tcggtctgtg gttgacatgc cgcccgacga
      121 cagcaatccg cgggcccgcc gtggcgcgcg tgatctcgcg ggcttgcgcg cctcgatcgt
      181 ccggctgccg gacagccgtc cgaaatccgg ccggacaccg gacaacgcct gagcagacct
      241 gcgccgtgaa gctggtcggt cgcgaggcgg tgatcgacaa gctggtctac acggcgaccc
      301 atctggtgca gggccacctg gtcgaccggg tccaccacgg gcctggcgtc aacggtccgg
      361 gcgcgctgct cgccgggcgt ccgctgcgcg cggcgcggcc gctgcattct tccggcccga
      421 cggcccgatg ctcgatgtgg tcgagctgcc gctcaccctc cccgccgagc tcggacccgc
      481 ccctgcggtg ccctcggacg ggtcctcccc gtggagggtt ccacaggtcg acctgaaacg
      541 aggttgcagg gacgctggag ggcggccgcg agaagtggaa ggatcgcgcc gggcggtggg
      601 gtagactcgt tcgacagaaa gcaggtcatc acatggacgc cgtacactcg cctgacgacc
      661 gggagccgcc gtccacagcg cgagcggatg cgcacgcggg gcacagcgcc gccgcgatga
      721 tccgcgcgcc gcggtcgctg gccccgccgg gcgtgatcaa cgtcgaggac ctgcggcggc
      781 gggcgaagcg acgcttgccc cgggtggtct tcaactacgt cgacggtggc gcggatgacg
      841 aggtcacgct gcgcgagaac tgctcggcct tccagggcgt gacgttccgg ccgcgccagg
      901 cggttgtggt cccgagccac gacctgcgca cgcgcgtgct gggctgcgag ctcgcgttcc
      961 ccgcgctgct cgccccgatc ggctacctcc gcctgatcca tcccgccggc gagctcgcgg
     1021 ccgcgcgtgc cgcgggcgcc gcggggacgg cgttcgtgca gtccacgatc tcgggacatg
     1081 cgctggagcg cacgcgggcg gcgtcgaccg gcccggtcgg gtatcagctg tacctggtcg
     1141 gcggccgggc ggcctccgag gctgcgatcg agcgcgcccg gcaggcaggg ttctcggcgc
     1201 tgttcgtcac catcgatacg ccggtcgccg gcctgcgcga gcgcgacctc cggggcggca
     1261 tgaaggagct gctgagcggc agcctcgcgg ccaagctgcg cttcctgccg cagttcttca
     1321 cgcgcccgcg gtgggtcgcc ggcttcctgc gcgacggcgg cctgccgagg ctcgtcaacg
     1381 tcgtgccacc cggcgagccg ccgatgcccc tggtcgacgt ggtcggagcg ctcgctcgct
     1441 cgggcgtcac ctgggaggac tttcgctgga tccgccaggc ctggaccggg ccgctggtgg
     1501 ccaagggcgt gctcaccggg gatgatgccc ggcgcgcgat cgatcacgga gcgactgcca
     1561 tcgtggtgtc gaaccacggc ggccgccagc tcgacagcgc gtccgcgacg ctgcgcgcgt
     1621 tgcccgaggt ggtcgccgcg gtgaacgggc aagccgaggt gctgctcgat ggcggcatcc
     1681 gccgcggcag cgacatcgtc aaggcgatct gcctgggcgc ccgcgccgtc ctggtgggac
     1741 gggcgtacgt gtacgggctc gccgcggcgg gggaggccgg cgtcgcacgg gcgctggaga
     1801 tcctgcgcgc cgacgtcgag cgcacgctca ggctgatcgg ttgcccgtcg atcgcggcgc
     1861 tcgacgcctc ctacgtcgag gcccggcccg ggtggcgatg aacctggtgt cgcgactggt
     1921 tcggtgacgg agcgcccgac ggcagcgctc agcggaacat gaagcgctgc ttggcagggg
     1981 tgaacacgag gctgaacgtg tcgaggcgcg tgttgccgcc gagcacgatg ccgcacgcag
     2041 gggtgcccga gggtaggagc ggccccttcc agatcgggct gccgctcgca cccttctccg
     2101 acatcagatc gtagtcgacc agctttccgc gccgccgcag gcacatgccg acgtcggtgt
     2161 gttggaagtt gaggtcgacc agctccgccg agtaaccact ggtgttgacg cactcgttcg
     2221 cggcgggagg cggcacgggg aagaaccgac cgaggatgcc cggtcgcatc ggatacccgt
     2281 gcggaggcgg cacgggatgc agcaccgcga gatcttccgc gaagaagacg tcgggcgacg
     2341 tcgaggacag ggccgcgacg cggagcaggt ctgggaacac gtgtacagcc gaaccggcta
     2401 cccggaggcg gccgaacggc tcgtcgtggt gggtcctgcc gggcggtgcg atcccggggc
     2461 cattcttggc tgggatcacg acgacgtcga tcgccccgag cacgacgtgc gcggcagtga
     2521 cgatgcgatt gtggtccacg tagaacccgg tgccctcgcc ctggaccagg ccggtgagcg
     2581 agggatcgat cgcgaccgcc gaaccggatg gatcatcgga ggggcggtcg gggggctgct
     2641 cgacgggcct gccgtactcg tcgacgatgc cgatcccggc cagcgagccc gcgcgcttca
     2701 gcacgaggat cttgcagatc gcagtgtgcg ggaacgagag caccgcgggc gccgtcaccc
     2761 cgctggcgaa gcggagctcg cgatcgggtc ggatgcaggc gccggccgcg ggcgtggtgc
     2821 tgctttcgtc ctcgaaccag cgggagggag agcgtcgagc ggatggcctg ccgacgagct
     2881 cggcgtagaa ccgctcggct tcgtcggcgt cgctcgtggt gccgggtgca cccatgagct
     2941 cggcgtagaa ccgctcggac tcgtcgtgct cgatccgccg gagccgcgcg atgtcacgcg
     3001 aaacttggga gctcatggca cggcagcggc ggtcgcgttc cggagagtga tcatacgcac
     3061 atcatgccgg agcttccatg cgcacaggta aacaaaccgg cggtcgaacg tcggccagaa
     3121 ggcatcggtg agctgggtcg tcctggcgag cgatgatccg tggaagcggc gcaacgtact
     3181 ctgcgtaagc tgtgttacga acggagggtc ccgacagcgt caggccactc gcggctgacg
     3241 tcgcgcccgt gcgcgttccg ggcgctgcgc ggatcgatct tccgacgtcc atcgctgacc
     3301 gtgctgtgcc cacgggccgg tcaatgcttt cgccgtcggg tgggccggtc gggtggcctg
     3361 ggcccgaaca ccgcggcctc gacccggctc atgcgccccg gcagatctgc gagcggctcg
     3421 acgctcgcct tgatctccgc tcgcatcgct tcgtggatcg ccgaggtatg gcgcgccagc
     3481 gcggcgagca accgctgctc ggtctgctgg atctgctgct gggtctgctg tgcgccgacg
     3541 tcgagcgcac gctcaggctg ctcggttgcc cgtcgatcgc ggcgctcgac gcctcctacg
     3601 tcgaggctcg gcccgggtgg cgatggacct ggaccatcgc ggcgtcgagc tgtacgcggc
     3661 gcagatcagc acgcagggaa gaacaagttg gaaaacggcg tcgtggtgcc ccagctgcca
     3721 ctcggctgac cgcagcccga ccacttctgg ccgaccagct gcgtcttgcc ggggtcgctg
     3781 taataggcaa tgacggtgag ggtgtttccg gtcggtatcg cccgagccgg agccgcgctg
     3841 acagcgagag taccgatggc gagcgccagt gcaaacagtg catttcttct gaacatgata
     3901 tatcctttct cggattcgac gatccactct aaaaagttca cgacgggaca gcgccgaaaa
     3961 tggtcctccc acccagcgct gcttggccgt ggtgcttgcg gtggatatta ctgtttcgcg
     4021 ggagacccgt acgagggttc tctcgaggaa tgatagaccg gcgcccagag ctgccgggga
     4081 caccgacact ggccatgttg cttttgtacg tcatcttaca agtcgatagg aatggatggt
     4141 tcgcgaccgc ggtcgacgag gacatcgctg gttgctgcgc cgatgcgagg gcgcgaccag
     4201 gcacccgacg cgccatgcgg tatcggcacg tgcgttctgc gatgctctgc gcggtcttca
     4261 gcggcccgga taccatcggc gtccgcgccg ccatttcgat ctcacgctgg cgccgtattc
     4321 gatctcgtag cttcacggcg cactacgtca tgtacagccc gccattgacc tggaaggtct
     4381 ggccggtgat gtagccgttg gcggctatct cgcagacgag gccggcgacc tcctcgacgc
     4441 ggccgaggcg cccgaccggg atccggtccg cgcgcgcctt cgcgctcgcg gacgtcatgt
     4501 ccgtctcgat cagcgcgggc gcaatcgcgt tcaccgtgac gccatcgcgc gcgagccacg
     4561 atgcgtagcc atgcatcagg ccgatcaggc cggccttcga cgcggcgtag tgtgggccca
     4621 cgatgccacc ggtctgtgcg gcagtcgacg tcacgtagat gatgcgtccc cagcgtcggg
     4681 cgcgcatgga cgcgatggcc ttgctcgaga ccaggaatgc cgagcgcagg ttgacggcca
     4741 tcgcctcgtc ccatcgcgcg agatccagcg tatcgatcgt cgccggattg gcgatgccgg
     4801 cgttcgacac caggacgtcg atcggtccga gcgcagcggt gacctcggcg aacaggcgct
     4861 cgacgtccgg ctcgcgcgat acgtcggcac cgaccgcgat cgcacgacga cccagggcct
     4921 cgacctggcg cacaacggcg gccgcctcct cgccgcgcgt ccggtagttg acagcgacgt
     4981 cggcaccgct ttcggcgagg ccgagcgcgg cggcccgtcc aatgccacgg ctgccgcctg
     5041 tgacgagggc gactcggccc tcgagagcga tggtcgttgc gcgcgctgtc atcggccgtg
     5101 acagcatttg tacttcatcc cgctgccgca cgggcgcgga tcatggcggc cgacctgcag
     5161 tgcatcggcc gcatcgacaa gcttcatgcg cccttccctg atgtccatcg agcacggtct
     5221 cgcgtgcggc agattcggcg tcgccgatct gccgatgccc atggtcgacg tggtcggggc
     5281 gcttgctcgc tcgggcgtca cctgggagga cttccgctgg atccgccagg cctggaccgg
     5341 gccgctggtg gccaaggggg tgctcaccgg ggatgatgcc cggcgcgcga tcgatcacgg
     5401 agtgacggct ggggttagcc gttcctccga ataacctcgt acgggtcttc cccgaccgtg
     5461 ctgcgcccac gggggccggt caatgctttc gccgtcgggt tggccggtcg ggtggcctgg
     5521 gcccgaacac cgcggcctcg acccggctca tgcgccccgg cagatctgcg agcggctcga
     5581 agctcgcctt gatctccgct cgcatcgctt cgtggatcgc cgaggtatgg cgcgccagct
     5641 cggcgagcaa ccgctgctcg gtctgctgga tctgctgctg ggtctgctgg acgagcccga
     5701 tcagctgctc ttgtccccgc tccaggttgc cgatccggtg ctcgccttgc tggatgcgcg
     5761 ccaccagggc tccaccccac agctccagct gggccttggt ggcgagcggg gcgatggcgg
     5821 cctggatttc ctgctggagc tcggccttgg tggcgagcgg ggcgatggcg gcctggatct
     5881 cctgctggag ctcggccttg gtggcgagcg gggtgatggc ggcctggagc tcctgctggc
     5941 gctcaagcgc caactcttgt ttgagctccc gccggaacag cccgagggcg tcctgcagct
     6001 cgccacgcgt caccggggtc gacatatcgg tggagtccat catcactaca cctcccccgg
     6061 gtcgaaggca agacaccgcg cttgcgctgc gctcggcgcg gcacgcgacc gagcttcgtt
     6121 gcttcgcagc cgcgcatgct cttgcgctgg ccggctcaag aagcagcgac cgatgacgag
     6181 acgatgatga cgatgatcga gaggatgaag atgcatgcgc gccggtacag caagccgctt
     6241 gccagcgcgc ggtgacctga ttccgccggg ttccaagacg aatcgtccgg taaccggaca
     6301 gcatgtccga aatccggccg gacaccggac aggcacccga gagagaaccg tttgtcggca
     6361 acgctttgca gctctctcgc gtacttggag ccgggccatg tcccgctgga gtcggtcgtg
     6421 tcccgctgga tcccgtggcg tctggccaag aaatggcgaa agggtccgag tccgcgggcc
     6481 gatgctggat aatttcgcca gagagttatc gctgatggct acgctagcgg agggaccacg
     6541 catgcacgcg gccccgccca agcgcgccag tggcgccccg gtgcgcgtac ttcgagcgcc
     6601 tggcgggccc ctggcgcgcc agcggccccg cgggcaccgc cgagcgcttg tccgaccgcg
     6661 agcgccgtgc tgcctacgat gtcttccggc agaacgcgca gccgcgtcag cgatggtcga
     6721 tggtcaacac cgcggtcgtg accccgtggg cgtcaggctt ggcaacgacc cggtcgtcga
     6781 cctcggtcgg tcggcgggta tcttcttgag ggtggctcgt ctccgggtcg cgcggaggag
     6841 tgagctgcgc tggcttgcgc gaggaatcgc gcggctgtgg cgggaccctg ggctcactgc
     6901 gggctgtgcg ttctctcgtc gtgtcgggcg ctggccgcga ttgctccgca cgagtgagtc
     6961 gaaccgcacg atcgagtgga gtcgccgctg cgagcacgat gaagctctcg aagcccatgg
     7021 ccgcatccag ccgccagcgc ttgccaggcg gcaattgctg acgtcctcgc ggcacgatca
     7081 cttccccggc ggcgggaaac aactcgctga tctctccctt tgcatcgacg tacatgacat
     7141 agacgtacgc gtcgtgatcc aggtccacgt acagcgcgac ctcgtcacct ctcctcaatt
     7201 gctgtctcgg agccagcggc gtgttcgtcg tctggttcgc ccgccgcacg tcggcggtca
     7261 acacgatgtt ggctgtcgcc ggtgcgcttg tggtggtggg tggccgtggc gactgcgccg
     7321 ctggctctgg cgccggcacg acccacacgg tgggggacca acgaggacaa ccggccaaca
     7381 atgttagggc aatgagtgtt cgcatgatca cctcgacaag gcacatcgaa atccagtgtg
     7441 gtacgctgca aatctggctt gtgctcgact gcgagctgca gcgcgtacga cgcttgcatc
     7501 gagctcgcga ttgccgccgc gaaagacttg attctcaccc tcggtgacct gcggatcgcg
     7561 gcactctccg cagtcagggt aaccgtcgct gaatcggtcc gcgacccact cagacgcatt
     7621 gccggcgaga tcatggacgc cattgggcgt tacatccgcc gcaaccgacg cgacatccgc
     7681 gagcggacgc gggtcgggac agttcgtccc gggcaccttg ccgatctgga gcgcgcatcc
     7741 gagctgctcc ggctccgggc tgttccccca tggatagcgc ctgcgctcga tgccgcgcgc
     7801 agcgtattcc cactctgcct ccgtgggcag ccgcttgtcc agcgccgcgc agtacgcctg
     7861 ggcgccggcc ggcgccaccc ctatcaccgg gtggcgttcg gtgcccggcc ggggctcgaa
     7921 tgaccggttg ccgcgctgaa gctcggacgc gtcgagattc agcagcaggc taccgttcat
     7981 gcgcacaagc tcgccatcgg acaccgtgct cgcgctcgac agccagcgcg cgaatgcctg
     8041 gtttgtcacc tctgtgcgat cgatggcgaa cggcgagagc gcaacggcgc gcactggccc
     8101 ctcgcgctcg aacagctcgc ggcggcaccc gcgatagcga tggctctggc aatcctggta
     8161 cgcggtttgg atctcctgtg gtgtactccc catctcgaac ttcaccgctg ggaagccaat
     8221 catgtccgcc ggaggcggct tgacccgaaa caccagccac gcgagtatcg ccgtcgcgat
     8281 cgcaagacct gcggcaacac ggcttgttgc cccgaccccc gccggccgca tcaccgcgag
     8341 cacgagctga ctggcatcgc tgccaccccg aacgacgagc tgtgggtgtt cgtcgtctcg
     8401 tagcccctcc agaccgatgg cggcgcagcc ctgctggtac gctaccgcga tcgactcccg
     8461 ctctcccagc ccgccataga agccgatcgc gaagctccgc gcggcgacgt cctggatcga
     8521 cccgttcatc cccagcacgc agtcgacgtg cccgaccagc gcctccgcct gcgatgcggt
     8581 gtagcaggca ttgagcacga ccagcttcac cgagggcccc gcggaccgga acgtcttgca
     8641 cagcgcctcg ggcgacacca tcacggcgcc gccgtccggt ccctggaagc acaggccatt
     8701 cggctcgtca ctgcccataa ctgggctcac atctcgccga gcttggacat cgcgttgcgg
     8761 ctcgttcgcg actcgctcgc cgtggccgct gaagtggacg acggtcggcc ggagcttgcg
     8821 aagctcgcgc agcagatcga gcggctccgc ggcccagcgc gagatgaact cgaagcgatc
     8881 cccggagcgg gcgcgctcga gctcgtgctg gatcgcgcgg gcctcgcgat cgagcgcgag
     8941 tcgatccgtg ccggctggat tggccgccac aaatagaatg acgtgcttca tcggacaccc
     9001 atttcacggt ctaggcgacg cactcgatcg aatcacgaca tcgccgggtc cagtcgctcg
     9061 cagagccgct gtgcgcaccg actccggatc ggatctgttc ctaacggact tctaacgtcg
     9121 taatcacggc atagctggcg cccatctcgt gggccggtcg gattgtggtc tctgcacgca
     9181 gagctgcgca catcctgccg gccggtggca catagcgaga gaaagacgcc gcgaccggat
     9241 gcacccggcc ggtaatcaca aggaggatgc acatgcacgg ttgtcgtcga attctgtggg
     9301 ttgccatctt gctcgtcgct ggttgcggca ttctgggcag tctccccaaa ctgccgacag
     9361 attccccgat gggtgccgcg cagcgcgcgc tcaatcgctt caccggcggc aagctcgacg
     9421 ccgagggggt caaaatgatc cgcgaggtcg ggccgatcgc cgagcacgcg atcgccgcga
     9481 tcagccgcgc cgtcgtcgag gtccgcgtgc acaacgttct cgaggaggca tcgcgtctag
     9541 tcgccgctgc cacccagagc ttctacaccg acgagtgcac caccgccgct gagtctgctg
     9601 ggaccggcgt cggcccggcc agcctgagcg atgtggtcgg gttcgagcgc gaagcgggtg
     9661 aggcgttcca gagcgccaat tacgagaagg ctgcgtcgct gatgcagagc gtctacgaga
     9721 cccaaaaggg gctactcggc gccgggcacc ctgtggtgct gcatctactc aacaaacggg
     9781 ccgccgtcca gctcgcggtc aacaactaca agcaagccac cgcgtttggc ttgaccgctc
     9841 tgcaacgtcg catgcagtcc ctccagggca tcaaggtgac gttcccgccc tcgcacgagg
     9901 aggtcgccgc tgcgctcgcg gtcgccgaat cggccagtac actggccagc atctacagtg
     9961 cctctggtgt atacgcagaa gccaagcaga actacgaaca tgccatcgag ttgcgcagtc
    10021 actatctggg tgttgaccac cagtgcgctg cccagtcgca gaacggactg ggggagttgc
    10081 tccagctgat gggtgactat ccggctgcgc gccgactgtt cgaatccgcg ctccgcacgc
    10141 gtgaaacaaa gctcggcccg cggcatcgcg acaccgcacg ctcgatcaat gacctcggtt
    10201 ccctccatcg ctccatcggc gatctgctcc acgcgaagga gttctatcgc cgcgcactcg
    10261 atgttcgtct cacgtttggc gacgatgacg tcgatgtcgc cgaatcgcac aataacctcg
    10321 gcgatctcta caagctccgc ggcgagtacg ccaacgccga atcggagtac cgccaagcat
    10381 tcgatatccg ccggaagaag ttcggagagg gtgtcgaggc cgcacagtct caaatcgcga
    10441 ttgccaacct ctaccagatg atgggcgact accgtcgcgc gcaagacctg tacaatatcg
    10501 tctgcgagat tcgccagaag gtcctcggtc ccgatcatcc cgacaccgcg gagtcactca
    10561 gcgcactcgg cgattcacac cgagcacagc gcgattacac cagggctgag tccgagtacc
    10621 gccaggccct cgagatccgg cggaacaagc tcggccccga ccacccgctg gtcgccagct
    10681 cgctggtcgc actcggcaac gtgcatctcg acctcggcga ctacgccaag gccgagtccg
    10741 agtataacca ggcccgcgac attcagcaga acaagctggg tgccgagcac cccgatgtcg
    10801 cgcagtcgca gcaccagctg ggcctcctgc atcaggccag gggtgactac gcagaggccg
    10861 agtcgctcct ccgctccgcg ctgtcgctgc gcgataccaa gctgggtgcc ggtcatccga
    10921 gcgtggccga atcgctcgtg gcgctggcga agctgatggt ggccaccggc cgacacccac
    10981 aggcgctcgc gtacgtgcat cgcgcattcg gcatctccga gcagctgttg cagagcgtcg
    11041 gcgcggtcgc cagtgagcgc gtggacgcat tgctctttgg cggcgccttc gggttgatcc
    11101 attccgggcg ctggcgtgga tccacgcagg tggcggcggc gcggcgggat cgtgacccac
    11161 ctgggccgga tgccgggatc cggatccgtc tggatccgtt ccgctggatc ggatggatcg
    11221 actggatccg gcggcggcgg cggttagcgg cgtcgatctg gcgaggcctg gaggaggcgc
    11281 cggatggtcg cggggtcgtg gtcctcgaag tcgtcctggg tgac
//
LOCUS       DASVOR010002062         6718 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_256158,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002062 DASVOR010000000
VERSION     DASVOR010002062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6718)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6718)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6718
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256158"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..898
                     /locus_tag="VF516_38355"
     CDS             <1..898
                     /locus_tag="VF516_38355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113676.1"
                     /translation="GRTEYALSFEGSETVIDVERAFLEYRHGHWMLAAGRTHAEFGYW
                     NNAFHHGRWLQLTINRPRVLRFEDEGGMLQVHQVGVTAVYGPERGERGLEVALGVGNG
                     HGPSIVDVQTLGDNNLAKSVLIRVGAAGLDHDALRFGVNLGIDQIAPEPSTVRPLLPG
                     TRIFELLSGAYLALRSDPWIAFSEVYNVLHRAVGRTWQVTDGFVIAGYRMGAFTPYAQ
                     LEARRGDGLFDPFYNPDPMASPEAAIPSNYVEGILGLHYDLSTWSALKLELSARRFQS
                     LDDGVVHDHDDYRVELNWSFGR"
     gene            complement(958..2829)
                     /locus_tag="VF516_38360"
     CDS             complement(958..2829)
                     /locus_tag="VF516_38360"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX8113677.1"
                     /translation="MTVKRFLILGLGGLGALSLAVACRSADRAAMSPDSGGGPPTGGS
                     DAGSPTGGSDAGSPTGGDDAGSSGGSDAGSPPDARPPGNGSGIDPSPSITFTQVGLAA
                     GINRATEPASAGTFVQNLDLAYGSWLADLDGDGRLDYYAVNHGQFPHMSGLFLNNGHG
                     GFGPNLFTVSFASAFGVQQVNPPNMGQSNEMRFVGDLTGDGKVDFFFEGWSGEGTLCV
                     NQGVAQHSDWTGPSFQCFDTDDGMAFADVNGDGKIDVLSMVVPDRSTFDPYPAYYAQT
                     ATYLWRLNNGDPNINNWPTTTDFLSLRTTDPGAAAAAAPFLDLNGDGIPDKIVGVPAP
                     AGARGDYDTVLSGQQVFLGQADRKSYRLLSNTGLEGATTPITRVEDINGDGCLDIGTD
                     ITGYRDNQTWYIQNRTGSTCNVTFTATERTALPFYPGFKHYAVDIDNSGQLSQAVVVH
                     AGWGHSDGLQGGVYIYRQLPDGSYTAIPPAQSGINLQEFYADNLAPGDWNDDGRIDLG
                     GSGDSDMPATDKGFGLWTSKLSTTNSWIKITLPTVTGFFAGSARIEVFEAGFLGDANH
                     LVTPPKVLFTGRAWASQVYHFGIGTRSAVDVRVTFPDGRQVTKTGVTPSSRLTLQPG"
     gene            complement(3077..3952)
                     /locus_tag="VF516_38365"
     CDS             complement(3077..3952)
                     /locus_tag="VF516_38365"
                     /inference="COORDINATES: protein motif:HMM:NF021631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8113678.1"
                     /translation="MNLRSVVAAGARVHPPGWLCDNLCYETMMGSVAYGVSSDSSDMD
                     VYGICVPPKDVVFPHLAGEILGFGTQIKRFDSWQEHHVAALGKSWDFAIFGIVKFFQL
                     AMENNPNVIDSLFTPRRCVLSSTAIGEYIREHRVDFLHKGAWHKFKGYAYSQLNKIKT
                     KTPAGKRVELVEQFGYDVKFAYHVVRLLLEVEQILVARDIDLERDREQLKAIRRGEWT
                     LDQLERWAEDKEKALEALYHTSTVPYGPDEEQIKRHLVHCLEMHYGDLSAAVVQPDEA
                     VVTLRKIAELTQRYR"
     gene            complement(4071..>6718)
                     /locus_tag="VF516_38370"
     CDS             complement(4071..>6718)
                     /locus_tag="VF516_38370"
                     /inference="COORDINATES: protein motif:HMM:NF014214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005615 - extracellular space [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M36 family metallopeptidase"
                     /protein_id="HEX8113679.1"
                     /translation="PTGIPDGSTPQLVPSNLVVMEAFNGPLDPWLATNATTTSGNNAE
                     SFADLDATRMFTPGDVRPAVKSGRVLNYVYDHTLGPTANATQSSAGAVNSFFVVNWLH
                     DWYYDSGFTERTGNAQLDNLGRGGVAGDPLLVTAQAGANTGLRNNADMSTPADGARPR
                     MRMFLWTAGTTTKLFAPTGTPRSEAFSAGPRNFDLVGDLIEVVDGTAPTNDACQPITN
                     AVAGKIVLVTFSGVCGSAATVNNAKAAGAIGIILADGALDDPRGFAGSAAANIPSVAM
                     GKTDGAALEAALAAGPVTIEITSAVQGPERDGDLDNGVVAHEWGHYLHHRLAVCGTQQ
                     CGGMSEGWGDFNALMMMARDGDNRDGTYAEGVYALSDGVTPDVGYFGIRRFPYSRDRT
                     KNDLSFRHISDANPLPTATPGHPGGANSEVHNTGEVWASMMLEAFNVLADAHGVTVAR
                     RRMADYVVAGLLLTPPEATVTEGRDGILAAASALDTDDMILMAAAFAGRGAGSCAVSP
                     PVTSATNTPVTESGTLAGKLAVGGMSLVDDGISCDHDGFLDPGESGNLRFTLANHGIL
                     AAEGVTVTASTTNTGLKLGAPISIAAMQPFTSASLSIPVTLLATAPRNTIVTIKLHVA
                     GQSTCDRNGIDATVSVKTGVDDVPNASNIDHAETRVTPWMATGDAVTSSIWGHATEAS
                     GNQLYFGADAGFPSDTQFTSPPLTVSTTQPFVLKFSHAFDLEASGGTLFDGGVVEISL
                     NGGATWNDVSAFGVTPGYTGALFVGSGNPIGGRQAYSGTSPGFPARTAVSLNFGTLFA
                     GLTVQVRFRIGTDASASQSGWFIDDVEADGITNTPFPIQVPEPSTCTAKASVPAESGV
                     FATRAAPATSLRAFDNGVCILNEMQ"
BASE COUNT          946 a         2274 c         2405 g         1093 t
ORIGIN      
        1 cggccgcacc gagtacgcgc tgtcgttcga gggcagcgag acggtgatcg acgtcgagcg
       61 cgcgttcctc gagtaccgcc acggacactg gatgctggcg gccggccgca cccacgccga
      121 gttcggctac tggaacaacg cgtttcacca cggccgctgg ctgcagctga cgatcaaccg
      181 gccgcgcgtg ctccggttcg aggacgaggg cggcatgctg caggtccacc aggtcggcgt
      241 cacggcggtg tacggccccg agcgcggcga gcgcggcctc gaggtcgcgc tcggcgtcgg
      301 caacggccac gggccgtcga tcgtcgacgt ccagaccctg ggcgacaaca acctcgccaa
      361 gtcggtgctg atccgcgtcg gcgcggccgg gctcgatcac gacgcgctcc ggttcggcgt
      421 caacctcggc atcgaccaga tcgcgccgga gccgagcacg gtgcgcccgc tgctccccgg
      481 aaccaggatc ttcgagctgc tgtcgggcgc gtacctcgcg ctgcgctcgg acccatggat
      541 cgcgttcagc gaggtctaca acgtcctcca ccgcgcggtg ggccggacct ggcaggtcac
      601 cgacggcttc gtcatcgcgg gataccgcat gggcgcgttc acgccgtatg cccagctcga
      661 ggcccggcgc ggcgatggcc tgttcgaccc gttctacaac ccggacccga tggcgtcgcc
      721 cgaggcggcg atcccgagca attacgtcga gggcatcctc ggcctgcact acgacctcag
      781 cacgtggagc gcgctcaagc tcgagctgtc cgcgcgccgg ttccagagcc tggacgacgg
      841 cgtggtgcac gaccacgacg attatcgcgt cgagctcaac tggtcattcg ggcggtgatc
      901 gggttccacg aggcgccgcc gccgatctcg ccgggctgag ccgcggcgga gcggacatca
      961 ccccggctgg agggtgagcc ggctgctcgg cgtgacgccg gtcttggtga cctggcggcc
     1021 atccgggaag gtcacgcgga cgtcgaccgc gctgcgtgtg ccgatgccga agtggtagac
     1081 ctggctggcc caggcgcggc cggtgaacag caccttgggc ggggtcacga ggtggttggc
     1141 gtcgcccagg aagccggctt cgaacacctc gatcctcgcc gatccggcga agaacccggt
     1201 gacggtcggc agcgtgatct tgatccagct gttcgtggtc gacagcttcg acgtccacag
     1261 cccgaagccc ttgtcggtcg cgggcatgtc ggagtcgccg ctgccgccga gatcgatccg
     1321 gccgtcgtcg ttccagtcgc ccggcgcgag gttgtcggcg tagaactcct ggaggttgat
     1381 gccgctctgg gccggcggga tcgcggtgta gctgccgtcg ggcagctggc ggtagatgta
     1441 gacgccgccc tgcaggccgt cgctatggcc ccagccggcg tggaccacca cggcctgcga
     1501 cagctgtccg ctgttgtcga tgtccaccgc gtagtgcttg aacccgggat agaacggcaa
     1561 ggcggtgcgc tccgtggccg tgaacgtgac attgcacgtg ctgccggtcc tgttctggat
     1621 gtaccaggtc tggttgtcgc gatagccggt gatgtcggtg ccgatgtcga ggcagccgtc
     1681 gccgttgatg tcctcgaccc gcgtgatcgg tgtggtggct ccctcgaggc cggtgttgct
     1741 cagcaggcgg tagctcttgc gatcggcctg gccgaggaac acctgctggc ccgacagcac
     1801 ggtgtcgtag tcgccgcggg cgccggcggg agccggcacg ccgacgatct tgtccgggat
     1861 gccgtcgccg ttgaggtcga ggaacggcgc cgcggcggct gcggcgccgg gatcggtggt
     1921 ccgcagcgac aggaagtccg tggtggtcgg ccagttgttg atgttcggat cgccgttgtt
     1981 cagccgccac aggtacgtcg cggtctgtgc gtagtacgcc gggtacgggt cgaacgtgct
     2041 gcggtccggg accaccatgc tcaggacatc gatcttgccg tcgccattga cgtcggcgaa
     2101 cgccatgcca tcgtcggtgt cgaagcactg aaagctcggg ccggtccagt cggagtgctg
     2161 ggcgacgccc tggttgacgc acagcgtgcc ctcgccgctc cagccctcga agaagaagtc
     2221 gaccttgcca tcgccggtca gatcgccgac gaaccgcatc tcgttggact ggcccatgtt
     2281 cggcgggttc acctgctgga ccccgaacgc gctcgcgaac gacaccgtga acaggttggg
     2341 gccgaacccg ccgtggccgt tgttgaggaa cagccccgac atgtgcggga attgcccgtg
     2401 gttcaccgcg tagtagtcga gccggccgtc gccgtcgaga tcggccagcc acgagccgta
     2461 ggcgaggtcg aggttctgga cgaacgtacc ggcggaggcc ggctcggtgg cgcggttgat
     2521 cccggccgcg agaccgacct gggtgaacgt gatgctcggg ctcgggtcga tgccgctgcc
     2581 attccccggt ggccgcgcgt ccggcggcga tccggcgtcg ctgccgcccg acgatcccgc
     2641 gtcgtcaccg ccggtcggcg atccggcgtc gctgccgccg gtcggcgatc cggcatcgct
     2701 gccgccggtc ggtggtccac cgccgctgtc cggcgacatc gccgcacggt ccgccgagcg
     2761 gcacgcgacg gccagcgaga gtgccccgag tccgccgagt ccgaggatca ggaatcgctt
     2821 cacggtcatt ccgcaggtcc cctggtcagt ccaaccaatg gtagatcgcc aatccccggg
     2881 cccacctcac cgatcgagca cgtcctgcat accgcgtgat gtagctctca ggtgaatcgc
     2941 gatccgattc tcacggatcc tcacgaatgg aggtagcaat gcatcagcgg ctgtcggccc
     3001 tgaacctgac atgccggatc cgggtatgca aaggtccgtg cggaccgcag gcgggcaaga
     3061 tgccccgcgg gtcgcctcag cgataccgct gggtcaactc ggcgatcttg cgcagggtca
     3121 ccacggcctc gtcgggctgc accaccgcgg ccgacagatc gccgtagtgc atctccagac
     3181 agtgcacgag gtggcgcttg atctgctcct cgtccggacc gtacggcacg gtgctcgtgt
     3241 ggtagagcgc ctccagcgcc ttctccttgt cctcggccca gcgctcgagc tgatcgagcg
     3301 tccactcgcc gcgccggatc gccttgagct gctcgcgatc gcgctccagg tcgatgtcgc
     3361 gcgcgaccag gatctgctcg acctcgagca gcaggcggac gacgtggtac gcgaacttca
     3421 cgtcgtagcc gaactgctcg acgagctcga cccgcttgcc ggccggcgtc ttggtcttga
     3481 tcttgttgag ctggctgtac gcgtagccct tgaacttgtg ccacgcgccc ttgtgcagga
     3541 agtcgacgcg gtgctcgcgg atgtactcgc cgatcgcggt cgacgacagc acgcagcgcc
     3601 gcggcgtgaa cagcgagtcg atcacgttcg ggttgttctc catcgcgagc tggaagaact
     3661 tcacgatgcc gaagatcgcg aagtcccacg acttgccgag cgccgcgacg tggtgctcct
     3721 gccacgagtc gaaccgcttg atctgcgtgc cgaacccgag gatctcgccg gcgaggtgag
     3781 ggaacacgac atccttgggc ggcacgcaga tcccgtagac gtccatgtcc gacgagtccg
     3841 acgacacgcc gtacgccacg ctccccatca tcgtctcgta acagaggttg tcgcacagcc
     3901 agcccggcgg gtggacccgg gctcctgcgg ccacgacgct gcgaaggttc atcgccgcag
     3961 acgttacggc acggggcgag cttcggcgag cgagccgtcg cggcgacccg cgcggtgggc
     4021 ccgggtcgct ggcgatggcc ggcgcttgcc gcggcgcggg gccggcggtg ttactgcatt
     4081 tcgttcagga tgcagacgcc gttgtcgaac gcgcgcaggc tggtcgccgg cgcggcccgc
     4141 gtcgcgaaca caccgctctc ggccggtacg ctcgccttcg ccgtgcaggt cgacggctcg
     4201 gggacctgga tcgggaacgg cgtgttggtg atgccgtcgg cctcgacgtc atcgatgaac
     4261 cagccgctct gcgaggccga ggcgtcggtg ccgatgcgga accggacctg gaccgtcagc
     4321 ccggcgaaca gcgtcccgaa gttcagcgag acggcggtcc gcgccgggaa gcccgggctc
     4381 gtcccgctgt acgcctggcg cccgccgatc gggttgcccg agccgacgaa cagcgcgccg
     4441 gtgtagcccg gggtgacgcc gaacgccgag acgtcgttcc acgtcgcccc gccgttgagc
     4501 gagatctcga ccacgccgcc gtcgaacagc gtcccgcccg acgcctcgag gtcgaacgcg
     4561 tggctgaact tgaggacgaa cggctgggtc gtgctgacgg tcagcggcgg cgaggtgaac
     4621 tgcgtgtcgc tcgggaagcc ggcatcggcg ccgaagtaca gctggttgcc gctggcctcg
     4681 gtggcgtggc cccagatcga cgacgtcacc gcgtcgccgg tcgccatcca cggcgtgacc
     4741 cgggtctcgg cgtgatcgat gttggaggcg ttgggcacgt cgtccacgcc ggtcttcacc
     4801 gagaccgtgg cgtcgatgcc gttgcggtcg caggtgctct ggccggcgac gtgcagcttg
     4861 atcgtcacga tcgtgttgcg cggcgcggtc gccagcaggg tcaccgggat cgacagcgac
     4921 gcgctggtga acggctgcat cgcggcgatc gagatcggcg cgccgagctt gaggccggtg
     4981 ttggtcgtcg atgcggtgac cgtgacgccc tcggcggcca ggatgccgtg gttggcgagc
     5041 gtgaaccgca ggttgccgct ctctcccgga tcgaggaagc cgtcgtgatc gcacgagatg
     5101 ccgtcgtcga ccaggctcat cccgccgacc gcgagcttgc cggccagcgt gccgctctcg
     5161 gtcaccggcg tgttggtcgc cgacgtgacc ggcggcgaca ccgcgcacga cccggcgccg
     5221 cggccggcga acgccgcggc catcaggatc atgtcgtcgg tgtcgagcgc gctcgcggcc
     5281 gcgaggatgc catcgcggcc ctcggtgacc gtcgcctcgg gcggggtgag caggaggccc
     5341 gccacgacgt agtcggccat ccggcggcgc gcgacggtga cgccgtgcgc gtcggccagc
     5401 acgttgaacg cttccaacat catgctcgcc cacacctcgc cggtgttgtg gacctcgctg
     5461 ttggccccgc cggggtggcc gggcgtggcg gtcggcagcg ggttcgcgtc ggagatgtgg
     5521 cggaagctga ggtcgttctt ggtccggtcg cggctgtacg ggaaccggcg gatgccgaag
     5581 tacccgacgt cgggcgtcac gccgtccgag agcgcgtaca cgccctcggc gtaggtgccg
     5641 tcgcggttgt cgccgtcgcg cgccatcatc atcagcgcgt tgaagtcacc ccagccctcg
     5701 ctcatcccgc cgcactgctg ggtgccgcag accgcgagcc ggtggtgcag gtagtggccc
     5761 cactcgtggg cgaccacgcc gttgtcgagg tcgccatcgc gctccgggcc ctggacggcg
     5821 ctggtgatct cgatcgtcac cggacccgcc gcgagcgcgg cctcgagcgc agcgccgtcg
     5881 gtcttgccca tcgccacgga cgggatgttg gcggcggcgc tgccggcgaa cccgcgcgga
     5941 tcgtccaggg cgccgtcggc caggatgatg ccgatcgcgc ccgcggcctt ggcgttgttg
     6001 accgtggccg ccgagccgca cacgccggag aacgtgacca gcacgatctt gccggccacc
     6061 gcgttggtga tcggctggca ggcgtcgttg gtcggcgcgg tgccgtcgac gacctcgatc
     6121 aggtcaccga ccaggtcgaa gttgcgcggt ccggcggaga acgcctcgct gcgcggggtg
     6181 ccggtcggcg cgaacagctt ggtggtcgtg cccgcggtcc acaggaacat ccgcatccgc
     6241 ggccgcgcac cgtcggccgg cgtcgacatg tcggcgttgt tgcgcaggcc ggtgttggcg
     6301 cccgcctgcg cggtgaccag cagcggatcg ccggcgacgc cgccgcggcc gaggttatcc
     6361 agctgcgcgt tgccggtccg ctcggtgaac cccgagtcgt agtaccagtc gtgcagccag
     6421 ttgaccacga agaacgagtt gaccgccccg gccgaggact gcgtcgcgtt ggcggtcggc
     6481 cccagggtgt gatcgtagac gtagttgagg acgcggcccg acttgaccgc cgggcggacg
     6541 tcgcccgggg tgaacatgcg ggtggcgtcg aggtcggcga acgactcggc gttgttgccc
     6601 gaggtcgtgg tcgcgttggt cgccagccac gggtcgagcg ggccgttgaa cgcctccatc
     6661 acgaccaggt tcgacggcac cagctggggc gtcgagccgt cggggatgcc ggtcgggt
//
LOCUS       DASVOR010002063         4457 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_226539,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002063 DASVOR010000000
VERSION     DASVOR010002063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226539"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1769
                     /locus_tag="VF516_38375"
     CDS             <1..1769
                     /locus_tag="VF516_38375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113680.1"
                     /translation="VSDDREAAEERCAARDPACDWTATLSSLERASVLRALAARGYEP
                     EPAPWGKVVGAVRVYTEEVFAEPSEFLQFFNYFHFRTKDSAIRAEAVVAPGQVWDQAR
                     VEETARLIRDPLWNSVVAVIPVKAAAPGTVDVLIVTRDVWSLRFNTQYTIQQGKLTNL
                     TTSLSENNFLGQRDVLSAALTMDQGAIAVGPLFIDKDVLGEHFNLQARVDDILTRDDL
                     TKHSTLHHEGSDSTITLTRPLWNLASEWAGGAQFTHRFAVNRQFFGTGLRQFKYTDPM
                     TGQSTLFPIEYGMRQWSANVFVTRQWGDELKQQLSIGHVITSQRPRPLDDFAGDAAQR
                     AAFIARVLPRSEVTSVPFLEYSFFTPRYRTLRNVSTFDLAEDQRTGPDLDVSFGFGLR
                     ALGSDHNFQRLSSSVGWTIPWARDGFVRIAGGLGGRYQERETDPMVPHGFIDDTATVS
                     IRGGAPPIGGVLRLLAQSSLTTRWNDTQNQFYAIGSDSGLRGYNINEFIGQRYFNLQV
                     EARTLPYPIWVLRIGGVVFYDLGGAADTLATIALHQDAGLGARVLIPQLSAQLLTFDL
                     AVPFDGAARGMPRFLAGVGSQF"
     gene            complement(1772..3097)
                     /locus_tag="VF516_38380"
     CDS             complement(1772..3097)
                     /locus_tag="VF516_38380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8113681.1"
                     /translation="MRSDELSAQIRLAIPLAAQQLGFQLMGTVDAALLGRYADSALAA
                     AGVGNSVLFAVTSIGMGLVMGLDTVLPQAVGARRHDAARRSLAAGLRLAMLVGLGCTL
                     VVLASPALLTLADVPPDVIRDARLYVDLRAIGIVPFLVSIALRSYLAAHHVTRPLIAA
                     VVLGNLVNAGLDVVLIYGAGPVPPLGVAGAAIATVSVQLGIVAIYRAGARAIDGGSER
                     PAATRADVVEILRYGAPIGGHLFAEIGVFALATVLAAHLGKLPAAAHSIALSLASFTF
                     SVALGIGSATAVRVGHAIGAGDRRLARRRGLLGLRIGLAVMAVFAAAFLIAPGALAGL
                     FTDSAPVIAQAVPLLQIAALFQLSDGSQAIAAGALRGIGNTRATFVGNVIGHYGVGLA
                     ISLALAFGAGLGAPGLWWGLSAGLTATALYLVQRFLAGTREPRGRADPA"
     gene            complement(3116..3754)
                     /locus_tag="VF516_38385"
     CDS             complement(3116..3754)
                     /locus_tag="VF516_38385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113682.1"
                     /translation="MRRFLSVLAVLAGCKDNSAAASDPPAAVAPPHLAGVYPEQWRCD
                     SVASPEAVGPLLGGTARPIESPASVPHGVPHPCSYEVLSPEPEYWTFDVDCRDGMKQR
                     ADALFAQYRETSREAMAHYAQLDAGVAAAGRPGDAGVAAHPPEPAVEVAVGAKGLDHH
                     GEGLIFIDDDAPCYVRVTGKDAARRLALARLIAKNLTLDNAPMVPRPSSARR"
     gene            complement(3814..4296)
                     /locus_tag="VF516_38390"
     CDS             complement(3814..4296)
                     /locus_tag="VF516_38390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113683.1"
                     /translation="MLDWLGSVQIRTRSSSRVELDLTRATAWTGWGLCALGIYLATIG
                     RAWITIAGVLVFFLGILLATLRRKLVFDRDEGLLRSEQRILGVRRRTAIPLFHLRAVV
                     VAARRGMYVAYVERRLGGTIHLDEARRSAPLLALAEAISEVAELRLVFDATTRVAASD
                     "
BASE COUNT          640 a         1607 c         1656 g          554 t
ORIGIN      
        1 cggtgtccga cgaccgcgag gccgccgagg agcgctgcgc ggcgcgcgat ccggcgtgcg
       61 actggaccgc gacgctgtcg agcctggagc gcgccagcgt gctgcgggcg ctggcggccc
      121 ggggctacga gcccgagccg gcgccgtggg gcaaggtggt cggcgcggtg cgggtctaca
      181 ccgaggaggt gttcgccgag ccgagcgagt tcctgcagtt cttcaactac ttccatttca
      241 ggaccaagga ctcggcgatc cgcgccgagg cggtggtcgc acccggccag gtctgggacc
      301 aggcgcgggt cgaggagacc gcgcggctga tccgcgatcc gctgtggaac tcggtggtcg
      361 cggtgatccc ggtcaaggcg gcggcgccgg gcacggtcga cgtcctgatc gtgacgcgcg
      421 acgtctggag cctgcggttc aacacccagt acacgatcca gcagggcaag ctgaccaacc
      481 tcacgacgtc gctgtccgag aacaactttc tcggccagcg cgacgtgctg tcggcggcgc
      541 tgaccatgga tcagggcgcg atcgcggtcg gcccgctgtt catcgacaag gacgtgctcg
      601 gcgagcactt caacctgcag gcccgggtcg acgacatcct gacccgcgac gacctgacca
      661 agcacagcac gctccaccac gagggcagcg acagcacgat cacgctgacc cggccgctgt
      721 ggaacctcgc cagcgagtgg gcgggcggcg cgcagttcac ccaccggttc gcggtcaacc
      781 gccagttctt cggcaccggg ctgcgccagt tcaagtacac cgacccgatg acggggcagt
      841 cgacgctgtt ccccatcgag tacggcatgc ggcagtggag cgccaacgtg ttcgtcaccc
      901 gccagtgggg cgacgagctc aagcagcagc tgtcgatcgg ccacgtcatc accagccagc
      961 ggccgcggcc gctcgatgac ttcgccggcg atgcggcgca gcgcgcggcg ttcatcgcca
     1021 gggtgctgcc gcggtccgag gtgacgtcgg tgccgttcct cgagtactcg ttcttcaccc
     1081 cgcgctaccg cacgctgcgc aacgtgtcga cgttcgacct cgccgaggac cagcggaccg
     1141 ggcccgatct cgacgtctcg ttcgggttcg ggctccgggc cctgggctcg gatcacaact
     1201 tccagcgcct gagctcgtcg gtcggctgga cgatcccgtg ggcgcgcgac ggcttcgtcc
     1261 ggatcgcggg cggcctcggc ggccgctacc aggagcgcga gaccgatccg atggtgccgc
     1321 acggcttcat cgacgacacc gccacggtgt cgatccgggg cggggcgccg ccgatcggcg
     1381 gggtgctgcg gctgctcgcc cagtcgtcgc tgacgacccg gtggaacgac acccagaacc
     1441 agttctacgc gatcggctcg gacagcgggc tgcgcggcta caacatcaac gagttcatcg
     1501 gccagcgcta cttcaacctc caggtcgagg cccgcacgct gccgtacccg atctgggtgc
     1561 tgcggatcgg cggcgtggtg ttctacgacc tgggcggcgc ggccgacacg ctcgcgacga
     1621 tcgcgctgca ccaggacgcc gggctcggcg cgcgggtctt gatcccgcag ctctcggcgc
     1681 agctgctcac gttcgacctc gcggtgccgt tcgacggcgc cgcccgcggc atgccgcggt
     1741 tcctcgccgg cgtcggctcg cagttctgaa cctacgcggg atcggcgcgg ccccgcggct
     1801 cgcgcgtgcc ggcgaggaac cgctgcacga ggtagagcgc ggtggcggtg aggcctgccg
     1861 acaggcccca ccacagcccg ggtgcaccca gaccggcgcc gaacgccagc gcgagcgaga
     1921 tcgccaggcc gacgccgtag tggccgatca cgttgccgac gaaggtcgcg cgggtgttgc
     1981 cgatgccgcg cagcgcgccg gcggcgatcg cctggctgcc gtccgacagc tggaacagcg
     2041 cggcgatctg gagcagcggg accgcctggg cgatcaccgg ggcgctgtcg gtgaacaggc
     2101 cggcgagcgc gccgggcgcg atcaggaacg ccgccgcgaa caccgccatc accgcgaggc
     2161 cgatccggag gccgagcagg ccgcgccggc gggccagacg gcggtcgccg gcgccgatcg
     2221 cgtggccgac ccggaccgcg gtcgccgagc cgatcccgag cgcgaccgag aacgtgaagc
     2281 tggcgaggct gagcgcgatc gagtgcgccg ccgccggcag cttgcccagg tgggcggcga
     2341 gcacggtggc gagcgcgaac acgccgatct cggcgaacag gtggccgccg atcggcgcgc
     2401 cgtagcgcag gatctcgacg acgtcggcgc gggtcgcggc ggggcgctcg cttccgccgt
     2461 cgatcgccct cgcgccggcg cggtagatcg cgacgatccc gagctgcacg ctgaccgtgg
     2521 cgatcgcggc gcccgcgacg ccgagcggcg ggaccgggcc ggcgccgtag atcagcacga
     2581 cgtcgagccc ggcgttgacc agattgccga gcaccaccgc ggcgatcagc ggccgcgtga
     2641 cgtggtgcgc ggcgaggtag gaccgcagcg cgatcgacac caggaacggc acgatgccga
     2701 tcgcgcgcag gtcgacgtac agccgcgcgt cgcggatcac gtcgggcggg acgtcggcga
     2761 gcgtgagcag cgccggcgag gcgagcacga ccagcgtgca gccgaggccg accagcatcg
     2821 ccagccgcag cccggcggcg agcgagcggc gcgcggcgtc gtggcgccgg gcgccgaccg
     2881 cctggggcag cacggtgtcg agccccatca cgaggcccat gccgatcgac gtcaccgcga
     2941 acagcacgct gttgccgacg ccggcggcgg cgagcgcgct gtcggcgtag cggccgagca
     3001 gcgcggcatc gaccgtgccc atcagctgga acccgagctg ctgcgcggcg agcgggatcg
     3061 cgagccggat ctgcgcggac agctcgtcgg agcgcatcgc ggggccggtc gggcgtcacc
     3121 gccgcgccga actcggcctc ggcaccatcg gcgcgttgtc gagcgtcagg ttcttcgcga
     3181 tcagccgggc gagcgcgagg cggcgcgcgg cgtccttgcc ggtgacccgg acgtagcacg
     3241 gggcgtcgtc gtcgatgaag atcaggccct cgccgtggtg gtcgaggccc ttggcgccga
     3301 ccgcgacctc gaccgccggc tcgggcgggt gcgcggcgac gcccgcatcg ccgggtcgtc
     3361 ccgccgcggc cacgccggcg tcgagctggg cgtagtgcgc catcgcctcg cggctggtct
     3421 cgcggtactg cgcgaacagc gcatcggcgc gctgcttcat gccgtcgcgg cagtcgacgt
     3481 cgaacgtcca gtactcgggc tccgggctca ggacctcgta gctgcacggg tgcggcacgc
     3541 cgtgcggcac cgaggccggg gattcgatcg gccgggcggt gccgccgagc agcgggccga
     3601 ccgcctccgg gctcgcgacg ctgtcgcacc gccactgctc ggggtacacc ccggcgaggt
     3661 ggggcggggc gacggcggcc ggcggatcgg acgcggctgc gctgttgtcc ttgcagcccg
     3721 caagcacggc caagaccgac aggaagcggc gcatcgcggc ccccagacgg tagcgcgagg
     3781 tgaggcgagg ctcaatgtgc cgcggcgcgc acgtcaatcg gaggctgcga cgcgggtggt
     3841 cgcatcgaat accaggcgga gctcggcgac ctccgagatc gcctcggcga gcgcgagcag
     3901 cggcgccgag cggcgcgcct catcgaggtg gatcgtgccg ccgagccggc gctcgacgta
     3961 ggcgacatac ataccgcggc gcgcagcgac caccacggcg cggaggtgga acagcgggat
     4021 cgccgtgcgc cggcgcaccc cgaggatgcg ctgctcgctg cgcagcagcc cctcgtcgcg
     4081 gtcgaacacc agcttgcggc gcagcgtcgc gagcaagatc ccgaggaaga acaccaggac
     4141 gccggcgatc gtgatccagg cccggccgat cgtcgcgagg tagatgccga gcgcgcacag
     4201 cccccagccg gtccacgccg tggcgcgggt caggtcgagc tccacgcggg agctggatcg
     4261 cgtccggatc tgtactgagc ccagccagtc gagcacgatc caagcttacc ctgaaccacc
     4321 gccgccgtcc gagatcgacc cgacgccccc gccgctcggg ggggaaccgc cgcctccgcc
     4381 gttgcccccg ttgcccccgt tgccgccgtt gccctgggtg cgccactcgc gctgccggcc
     4441 accgcggccg cgccggc
//
LOCUS       DASVOR010002064         2858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_283867,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002064 DASVOR010000000
VERSION     DASVOR010002064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283867"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF516_38395"
     CDS             complement(<1..347)
                     /locus_tag="VF516_38395"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8113684.1"
                     /translation="MLLPEYDDQDATGLAELVRTRQLSARELVDAAIAHIEARNPALN
                     AVVHTQFERARREAQHAGAGSFAGGPFLLKDLFAEEAGEPSTGSCQLGSAWRADRDSE
                     LVARYKRAGLIILG"
     gene            complement(394..1407)
                     /locus_tag="VF516_38400"
     CDS             complement(394..1407)
                     /locus_tag="VF516_38400"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8113685.1"
                     /translation="MPDTAYASIGDLALRHGGVVRDARLAYVAHGTLAHDGRNAVLLT
                     HGYTSSHRFADGGAAAEGSWSALVGPGRAIDTDRFFVVSSNMLGSSFGSTGPGSPDPA
                     TGRPYGPDFPRLTLADIVAAQRRLVEGLGVRQLAAVVGPSYGGFQAFAWGVEYPDFVR
                     AIAPVTTAPRMAGAIDLAPLRARLAADPAWNGGRYAAGAMVDTMTALREDTLRRYGIE
                     AALSEYTADPAARAEALRAIARRWAEVFDPHSLFVLGDAANQFDATPQFHRLRARVLF
                     VLSTTDALFPPALTAPVMAALRAAGVEASYEELDSPYGHLAPGLDAAKWAPALARLMA
                     SVA"
     gene            1912..>2858
                     /locus_tag="VF516_38405"
     CDS             1912..>2858
                     /locus_tag="VF516_38405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113686.1"
                     /translation="MSTRDRTRTGCLIAVVAIASSFAWHREAPGREADRDHDQDQDQD
                     GDRDAAEDPGANDYFYLQRRLPDGRIGLQAHARALAHARLKRAQLMATSGLDATGVWQ
                     LRGPVNVGGRVTDVVADPANANKFYVASASGGVWKTTDGGTTFTPIFDGLNTLSIGAL
                     ALDPRDSNILYVGTGEANPGGGSTTQPGDGVWKTTDGGTTWQHLGLDNTFEIGRIVVD
                     PKNPNNVFVAAMGALFFHNIDRGVYRSQDGGLTWTKVLFVSDGAGAIDLAIDPVTPTR
                     IFAATWERQRTPSARIYGGPGSGLWRSTDGGTTWTRLAGG"
BASE COUNT          403 a         1012 c         1048 g          395 t
ORIGIN      
        1 ccgaggatga tcaggccggc gcgcttgtag cgggcgacca gctcggagtc gcgatcggcc
       61 cgccaggcgc tgccgagctg gcagctgccc gtgctcggct cgcccgcctc ctcggcgaac
      121 agatccttga gcaggaaggg ccccccggcg aagctgccgg ccccggcgtg ctgggcctcg
      181 cggcgcgcgc gctcgaactg ggtgtggacc accgcgttga gcgcggggtt gcgcgcctcg
      241 atgtgcgcga tcgcggcgtc gaccagctcc cgggcggaca gctggcgggt gcgcaccagc
      301 tcggccagcc cggtggcgtc ctggtcgtcg tattcgggga gcagcatgga ggctcgcagg
      361 ctactacgcc gctcgggtcc gcgggacctc gcgctacgcg acgctcgcca tcagccgcgc
      421 cagcgccggt gcccacttcg ccgcgtcgag gccgggagcc aggtggccgt aggggctgtc
      481 gagctcctcg tacgaggcct cgacgccggc ggcgcgcagg gcggccatga ccggcgccgt
      541 gagcgccggc gggaacagcg cgtccgtggt cgacagcacg aacagcacgc gggcgcgcag
      601 ccggtggaac tgcggcgtcg cgtcgaactg gttggcggcg tcgcccagca cgaacagcga
      661 gtgcggatcg aagacctcgg cccaccggcg cgcgatcgcg cgcagcgcct cggcgcgcgc
      721 ggccgggtcg gcggtgtact ccgagagcgc ggcctcgatg ccgtagcgcc gcagggtgtc
      781 ctcgcgcagc gcggtcatcg tatcgaccat cgcgcccgcc gcgtagcggc cgccgttcca
      841 tgccgggtcc gcggcgagcc gcgcgcgcag cggtgcgaga tcgatcgcgc cggccatccg
      901 cggcgcggtg gtgaccggcg cgatcgcgcg cacgaagtcg gggtactcga cgccccacgc
      961 gaacgcctgg aacccgccgt acgatggccc gaccacggcg gcgagctggc ggacgccgag
     1021 cccctcgact agccggcgct gcgccgcgac gatatcggcc agcgtcagcc gcgggaaatc
     1081 ggggccgtag ggccgcccgg tcgccggatc cgggctcccc gggccggtcg agccgaagct
     1141 cgagcccagc atgttggagc tcacgacgaa gaatcgatcg gtgtcgatcg cgcggccggg
     1201 accgaccagc gcgctccacg agccctcggc cgccgcgccg ccgtcggcga accggtggct
     1261 gctggtgtag ccgtgggtca ggagcacggc gtttcgcccg tcgtgcgcca gcgtgccatg
     1321 tgcgacgtag gccagcctgg cgtcgcgcac caccccgccg tggcgcagcg cgagatcgcc
     1381 gatcgatgcg tacgcggtgt cgggcatggc ggcgatacta gcgcgcgacg ggcgcagcgg
     1441 ccgcgccagg gtcgccaggg ggccggcggc tcctcgccgg ggatgagctt cgggcagccc
     1501 gggcccgcaa acgcagctcg tcgagccatg gcaggcggag gtggccggcg cggatctcgc
     1561 gggtgagcgg gcggctcaca gctcgcactc gcgcccggcg ggcgcatcag aatgccaatc
     1621 tcgaccggtg actcccgttc tcgcaattgc caggatttgc tggcgtcacc gacgggagcg
     1681 gtttcttgga tccggataga cgtattactg ctgacctgct gccatcgcgg tgatcgggga
     1741 gtcggggtca ccgcgatgac gcgtagcgac atcctgtact gtcttttcac cctgtagtca
     1801 cggaacgagg ttgacgtggg ggtttcggcc caggcacgtt tctcgccgct cgaggtacgc
     1861 cgagcaccgg cgcccgtggc tccggcccat ggctctcgga aggagtttcg tgtgagcact
     1921 cgtgatcgaa cccgtactgg ctgtctgatt gctgtcgtcg ccatcgcgtc ttccttcgcc
     1981 tggcaccggg aagcgcctgg acgcgaggcg gaccgcgacc acgaccagga ccaggaccag
     2041 gatggcgacc gcgacgcggc cgaagatccg ggcgccaacg actacttcta tctccagcgc
     2101 cggctgcccg acggccggat cggcctgcag gcgcacgcgc gcgcgctggc acatgcccgg
     2161 ctgaagcggg cgcagctgat ggcgacgtcc gggctcgacg cgacgggggt ctggcagctg
     2221 cgcgggccgg tcaacgtcgg cggacgcgtg accgacgtgg tcgccgatcc tgccaacgcg
     2281 aacaagttct atgtcgcgtc cgcgagcggc ggcgtgtgga agaccaccga tggcggcacg
     2341 acgttcaccc cgatcttcga cggtctgaac acgctctcga tcggcgcgct cgcgctcgat
     2401 ccacgcgatt cgaacatcct ctatgtcggc accggcgagg ccaacccggg cggtggcagc
     2461 accacccagc cgggcgacgg cgtgtggaag accaccgacg gcggcacgac ctggcagcac
     2521 ctcgggctcg acaacacgtt cgagatcggc cgcatcgtgg tcgaccccaa gaatcccaac
     2581 aacgtgttcg tcgccgcgat gggcgccctg ttcttccaca acatcgatcg cggcgtctac
     2641 cggtcgcagg atggcggctt gacctggacc aaggtgctgt tcgtgtccga cggcgcgggc
     2701 gcgatcgatc tcgcgatcga cccggtcacg ccgacccgca tcttcgccgc gacctgggag
     2761 cgccagcgca cgccctcggc gcggatctac ggcggcccgg gcagcggcct gtggcgctcg
     2821 accgacggcg gcacgacgtg gacccggctg gccggcgg
//
LOCUS       DASVOR010002065         2141 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_226577,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002065 DASVOR010000000
VERSION     DASVOR010002065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226577"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..791)
                     /locus_tag="VF516_38410"
     CDS             complement(81..791)
                     /locus_tag="VF516_38410"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEX8113687.1"
                     /translation="MQRLFGGPVLAAALSICVAIAGGAAARAQPAPARPSDAPLPSCL
                     DQTIKDQLGADLKPRGVQKRDFLKNKKIVLIAHGGLYSGDMTSSNWIAGGSLGFFFTE
                     DLGAQVELDLTPLTLDLDAPLNKFFGDSHFNPGMAYLGLANLLWSPIHAKLKLGGGIV
                     HSDIMVFAGGGRMFHDAVQGLSFDAGGAIDLFVTRALTVRIDLRDLMAVEEISGETRF
                     TNNLIATAGLALYLPSGL"
     gene            981..>2141
                     /locus_tag="VF516_38415"
     CDS             981..>2141
                     /locus_tag="VF516_38415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8113688.1"
                     /translation="MEHLTGTRIRREATPATVNLRRCKLVVIKGAQRGTEFVIAGDVF
                     RVGKAAENDLVLSDETVSRVHFEIMRDAKGYLVRDLRSTNGTFLDGAEVKEAYLRAGS
                     VIGLGACELKFTPFEERIEILPSEKEALGEMVGRSPAMREIFGLVERIAPTDATVLIE
                     GETGTGKDLIARTLHQLSPRAGASFIVVDCGAVAGTLIESELFGHEKGAFTGAVAARQ
                     GAFELASGGTVFLDELGELSLDLQPKLLRVLEQRELRRVGGTKTIKVDLRVIAATRKD
                     LRSEVEKGKFREDLFFRLNVVPITAPALRERREDIPLLIDAMLGKLAPAGGIALSDAT
                     RAALMAHDWPGNVRELRNVIERALALGADPGTLVAPLGGEATARTAQLRDGIE"
BASE COUNT          336 a          729 c          790 g          286 t
ORIGIN      
        1 gcccgccggc gcgtcgtcgg cgcgcgccgc gagcggcacc gcgatccatc ccgccaggaa
       61 gacgccgatc gccgcgcgca tcacagcccc gacggcaggt acagcgcgag cccggcggtc
      121 gcgatgaggt tgttggtgaa ccgggtctcg cccgagatct cctcgacggc catcaggtcg
      181 cgcaggtcga tccggacggt cagcgcgcgg gtcacgaaca ggtcgatcgc accgccggcg
      241 tcgaacgaca ggccctggac cgcgtcgtgg aacatccggc cgccgccggc gaacaccatg
      301 atgtcggagt ggacgatgcc gccgccgagc ttgagcttgg cgtggatcgg cgaccacagg
      361 aggttggcga ggccgaggta cgccatgccg ggattgaaat ggctgtcgcc gaagaacttg
      421 ttgagcggcg cgtcgaggtc gagcgtgagc ggcgtcaggt cgagctcgac ctgcgcgccg
      481 aggtcctcgg tgaagaagaa cccgagcgag ccgccggcga tccagttcga cgaggtcatg
      541 tcgccgctgt acagcccgcc gtgcgcgatc agcacgatct tcttgttctt gaggaagtcg
      601 cgcttctgga ccccgcgcgg cttcaggtcg gcgccgagct ggtccttgat cgtctgatcg
      661 aggcacgacg gcagcggcgc atcggacggc cgggccggcg cgggctgcgc acgggcggcc
      721 gcgccgccgg cgatcgcgac gcagatcgag agcgcggccg cgagcacagg tcccccgaac
      781 aaacgctgca ctcactggaa ggtactctcg ttggatccac caaacaagaa accgggccgg
      841 agatccgggg ccgcggcggt gccagcagat gccacgcagg tgggatgtga gtgaacgcag
      901 ggcggccgcc gcggcgcaat cggtgcggcg gatcggtgcc gccgcgagac gtgcgagtgt
      961 gatcgcggta ccatgtcaag atggagcacc tgacggggac gcgcatccgg cgtgaagcga
     1021 cgccggcgac cgtcaacctc cggcgctgca agctcgtcgt gatcaagggt gcgcagcgcg
     1081 gcaccgagtt cgtgatcgcc ggggacgtgt tccgggtcgg caaggccgcg gagaacgacc
     1141 tggtgctcag cgacgagacc gtgtcgcgcg tccacttcga gatcatgcgt gacgccaagg
     1201 gctacctggt gcgcgacctc cggtcgacca acggcacgtt cctcgacggc gccgaggtca
     1261 aggaggccta cctgcgcgcc ggctcggtga tcggcctggg cgcctgcgag ctcaagttca
     1321 cgccgttcga ggagcggatc gagatcctgc cgagcgagaa ggaggcgctc ggcgagatgg
     1381 tcggccggtc gcccgcgatg cgcgagatct tcggcctggt cgagcggatc gcgccgaccg
     1441 acgccaccgt gctgatcgag ggcgagaccg gcaccggcaa ggacctgatc gcccggaccc
     1501 tgcaccagct gtcgccgcgc gccggcgcgt cgttcatcgt cgtcgactgc ggcgcggtgg
     1561 ccgggaccct gatcgagagc gagctgttcg gccacgagaa gggcgcgttc accggcgcgg
     1621 tcgcggcgcg ccagggcgcg ttcgagctcg cgagcggcgg cacggtgttc ctcgacgagc
     1681 tcggcgagct atcgctcgat ctccagccca agctgctccg cgtgctcgag cagcgcgagc
     1741 tgcgccgggt cggcggcacc aagacgatca aggtcgacct ccgcgtgatc gcggcgaccc
     1801 gcaaggacct gcgctccgag gtcgagaagg gcaagttccg cgaggacctg ttcttccggc
     1861 tcaacgtcgt gccgatcacc gcgccggcgc tgcgcgagcg ccgcgaggac atcccgctcc
     1921 tgatcgacgc gatgctcggc aagctcgcgc cggccggcgg catcgcgctg tcggacgcga
     1981 cccgggccgc gctgatggcg cacgactggc cgggcaacgt ccgcgagctc cgcaacgtga
     2041 tcgagcgcgc gctcgcgctg ggcgcggatc ccggcacgct ggtcgccccg ctcggcggcg
     2101 aggccaccgc ccggaccgcg cagctgcgcg acggcatcga g
//
LOCUS       DASVOR010002066         3238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_430327,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002066 DASVOR010000000
VERSION     DASVOR010002066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430327"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="VF516_38420"
     CDS             complement(<1..375)
                     /locus_tag="VF516_38420"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8113689.1"
                     /translation="MATPSKDTIRYDDAIVRKFVTATIVWGAVGMGVGLWCALQLAMP
                     RLNFAPYFTFGRIRPLHTNAVIFAFAGNAIFAAVYYSSQRLLKARPLARLSQIHFWGW
                     QAIIVAAAITLPLGFTQGKEYAE"
     gene            complement(487..903)
                     /locus_tag="VF516_38425"
     CDS             complement(487..903)
                     /locus_tag="VF516_38425"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8113690.1"
                     /translation="MTTPLTPSRALDMLAAQHATLREMIDRCEALADEVDAGTAGASQ
                     LLREVARLRIAFDDHNQFEEQLLRPLLLDVEWLGAVRVSRMVEDHVEEHRAMRLGLAA
                     TPTGDLRGVLASLRDHLATEERYFLSRKVLRDDLAG"
     gene            complement(926..2341)
                     /gene="hemN"
                     /locus_tag="VF516_38430"
     CDS             complement(926..2341)
                     /gene="hemN"
                     /locus_tag="VF516_38430"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEX8113691.1"
                     /translation="MPDTDVATCLAEYASRGPRYTSYPPATEFGPVPLDQVERELTTI
                     GDRGEPISLYAHVPFCKSLCWYCGCNVIATRNENRGAAYLDRLMTEMELLASYVCGAP
                     VTEIAIGGGSPNFLAPRLLRTMIAALDRTFAVAPDARRSIELDPRTTTTAQVDVLAAG
                     GFRSLSMGVQDFSEAVQDAIHRHQSTLQTRWLVDRARTAGFDDVNIDIVYGLPLQTET
                     SFARTLDAVIDLAPDRIALFGYAHLPSKLPHQRLVERAGRVLDRYERATLLLLGIEKL
                     TGAGYLHLGLDHFARPGSRLARADAERRMVRTFQGYVEHRADAILGMGVSAISSTPRM
                     HWQSHGELPAWEQGVAAGQLPVERGFMLDDDDRLRRAVIGELMCRGEVDLARVAADHG
                     VDAASYFAREIEALGALDELARYDAAAGRVRTTPLGRLLVRNLCMVFDRYHREPTGLP
                     GVPDGASNGASSDAPRFSSTI"
     gene            2748..>3238
                     /locus_tag="VF516_38435"
     CDS             2748..>3238
                     /locus_tag="VF516_38435"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HEX8113692.1"
                     /translation="MSRILIAYASSFGQTRKIASTLAAELRSRGHEVELADALLAPPP
                     PVEDYDAVVIGSRIQFGLHARPLLEYVIDNRAELHEIPSYAFVVSMAAAGATSADPDG
                     YVAKLLAITHWQPRAAVAIAGGLPYRSYHWFLRFVMKLISRSAGHSTDTRRDHEYTDW
                     ARV"
BASE COUNT          458 a         1215 c         1120 g          445 t
ORIGIN      
        1 ctcggcgtac tccttgccct gggtgaaccc gagcggcagg gtgatcgccg ccgcgacgat
       61 gatcgcctgc cagccccaga agtggatctg cgacagccgg gcgagcggcc gcgccttgag
      121 cagccgctgc gacgagtagt acaccgcggc gaagatcgcg ttgccggcga acgcgaagat
      181 caccgcgttg gtgtgcagcg gccggatccg gccaaacgtg aagtacggtg cgaagttgag
      241 ccgcggcatc gccagctgca gcgcgcacca cagccccacc cccatcccga ccgcccccca
      301 gacgatcgtc gcggtgacga acttgcggac gatcgcgtcg tcgtaccgga tcgtgtcttt
      361 gctcggcgtc gccatcgcgc acggtcatcg caactgccgt gcctgcgccg gacggccctc
      421 ccgctcccac cgcgcgcagc ctttgcgctg acgcccggtc gcggcgcacc gcgtgcacca
      481 gctgggctag ccggcgaggt cgtcgcgcag aaccttccgc gacaggaagt agcgctcctc
      541 ggtcgcgagg tggtcgcgca ggctggccag cacgccgcgc aggtcgccgg tcggcgtcgc
      601 ggcgagcccg agccgcatcg cgcgatgctc ctccacgtgg tcctcgacca tgcgggagac
      661 ccgcaccgcg ccgagccact cgacgtcgag caagagcggc cgcagcagct gctcctcgaa
      721 ctggttgtgg tcatcgaacg cgatccgcaa ccgcgcgacc tcgcgcagca gctgggacgc
      781 accggcggtg ccggcgtcga cctcgtcggc gagcgcctcg cagcggtcga tcatctcgcg
      841 cagggtggcg tgctgggccg cgagcatgtc gagcgcgcgg ctgggggtca gcggcgtggt
      901 catgaaggcc tccgtgggtt ggggctcaga tcgtcgacga gaaccgcggc gcatcgctcg
      961 aagctccgtt cgaggccccg tccggcacgc cgggcaggcc ggtgggctcg cggtgatagc
     1021 ggtcgaacac catgcagagg ttgcggacca gcagccgccc gagcggggtg gtccgcaccc
     1081 ggccggccgc cgcgtcgtag cgcgccagct cgtcgagcgc gcccagcgcc tcgatctcgc
     1141 gcgcgaagta gctcgcggcg tcgaccccgt ggtcggccgc gacccgggcg agatcgacct
     1201 cgccgcggca catcagctcg ccgatcaccg cgcggcgcag ccggtcgtcg tcgtcgagca
     1261 tgaacccgcg ctcgaccggc agctgcccgg cggcgacgcc ctgctcccac gccggcagct
     1321 cgccgtggct ctgccagtgc atgcgcggcg tcgacgagat cgccgacacg cccatcccca
     1381 ggatcgcgtc cgcgcggtgc tcgacgtagc cctggaacgt ccgcaccatc cggcgctcgg
     1441 cgtccgcgcg cgccagccgc gagcccggcc gcgcgaagtg gtcgagcccg aggtgcaggt
     1501 agccggcgcc ggtcagcttc tcgatcccca gcagcagcag cgtcgcgcgc tcgtagcgat
     1561 cgagcacccg gccggcgcgc tccaccagcc gctggtgcgg cagcttgctc ggcaggtggg
     1621 cgtagccgaa cagcgcgatc cggtccggcg ccaggtcgat caccgcgtcg agcgtccggg
     1681 cgaagctcgt ctcggtctgg agcggcaggc cgtacacgat gtcgatgttg acgtcgtcga
     1741 acccggcggt ccgcgcgcgg tcgaccagcc agcgggtctg cagcgtgctc tggtggcggt
     1801 ggatcgcgtc ctggaccgcc tcggagaagt cctgcacgcc catgctgagc gagcggaagc
     1861 ccccggcggc cagcacgtcg acctgcgccg tggtcgtcgt ccgcggatcg agctcgatcg
     1921 agcgccgcgc gtcgggagcg accgcgaagg tgcggtcgag cgccgcgatc atcgtgcgca
     1981 gcaggcgcgg tgccaggaag ttcggcgatc cgccgccgat cgcgatctcg gtgaccgggg
     2041 cgccgcacac gtagctcgcg agcagctcca tctcggtcat cagccgatcg aggtaggcgg
     2101 cgccgcggtt ctcgttgcgg gtcgcgatca cgttgcagcc gcagtaccag cacaggctct
     2161 tgcagaacgg cacgtgggcg tacagcgaga tcggctcgcc gcggtccccg atcgtcgtga
     2221 gctcgcgctc cacctggtcc agcggcaccg gaccgaactc ggtcgccggc gggtagctgg
     2281 tgtagcgcgg gccacggctc gcgtactcgg caaggcaggt ggcgacatcg gtgtctggca
     2341 tgccgccagg aacatcaagg ttcgtgccgg ccggaccccg gggtgccgcg gaccgatcgc
     2401 gcagatctcg cgcggaggcg cagcggcgct tgcagccgct gcagacgccg agatggcacg
     2461 tcggctgctg gactcctggc catgacgacc cacgtcaagc gcccgagcga gggctggacc
     2521 atccccctgc acgtcatcgt gtggaccgcg atcaccgtcg ggttcatcat gaccctcgtc
     2581 gtcctgatga tgcactgacc cgccccgcgc cgccccgccg gcccgcgcag tccacgcacc
     2641 tcgcgccccg ctgcgcgcag gccttgcgca tcttccgcgc cgcgctccgc caagccgccg
     2701 cggcgccgga cgtccggctg gtcagatgct tgcacgcccg gtgcgacatg agccgcatcc
     2761 tcatcgccta cgcgagcagt ttcggtcaga cccgcaagat cgccagcacc ctcgccgccg
     2821 agctgcgcag ccgcggtcac gaggtcgagc tcgccgacgc gctgctggcg ccgccgccgc
     2881 ccgtcgagga ctacgacgcc gtcgtgatcg gctcgcgcat ccagttcggc ctccacgcgc
     2941 ggccgctgct cgagtacgtc atcgacaacc gcgccgagct ccacgagatc ccgagctacg
     3001 cattcgtcgt cagcatggcg gcggcgggcg ccacctccgc cgatcccgac ggctacgtcg
     3061 cgaagctgct cgcgatcacc cactggcagc cgcgcgccgc ggtcgcgatc gccggcggcc
     3121 tgccctatcg cagctaccac tggttcctgc gcttcgtgat gaagctgatc agccggtccg
     3181 ccggccacag caccgacacc cgccgcgacc acgagtacac cgactgggcc cgggtcca
//
LOCUS       DASVOR010002067         2015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_283907,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002067 DASVOR010000000
VERSION     DASVOR010002067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283907"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="VF516_38440"
     CDS             <1..447
                     /locus_tag="VF516_38440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113693.1"
                     /translation="ICPPEAIALGDAIVAHMEPTIAASPAAIQRGLAIGLVLYDLGAL
                     PRHARRARALTGDRAERYYERWLDGPTAIHRQLARGVKQLMSLSCYEQPEMMDRVGYR
                     PAPWVAEVTQRRLTVYQDDVQRQAEQLIAPDPLRPGVRVRPGKEPS"
     gene            447..1991
                     /locus_tag="VF516_38445"
     CDS             447..1991
                     /locus_tag="VF516_38445"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEX8113694.1"
                     /translation="MVAARPAVHEGAPPPGVFTRNDHHGDSVLDADAVIVGSGAGGAT
                     MAAELAEAGFDVVVLEEGSYYQTRDFTADSSAMARQLYRDGGATAALGDPPILYQEGS
                     AVGGSTVINGGMSWRTPDKILARWERELGLAGIARDLEPYFARVERRIHVAKMDPDVI
                     GTDNWLLKKGADAKGWKVIDNLRNQVHCVGSNRCAFGCPTGAKQSALVSYIPRALHYG
                     ARVYADVRVDRITRHGKRATGVVGTVKGTGHRITVRARLVVAACGAIHTPALLVRSGF
                     RSPSGQLGHNLSMHPNVKVVAIFDQPITGWKGTHQAFQVREFQDEGLLFAAVNLPPSI
                     LAMSFPHRGAALGELMDQYDHMVTAGMLCEDTTTGRVRTIDGKPQAFYQLAGADAANL
                     KRGVVLLSELLFAAGARRILLPFHGARDLYSPDDARRLLDQPIPIKGWEVVTVHMMGT
                     ARMGADRSHAVTSPFGMVHDADRLMIADASLFPTPIGVNPMETIMALATRGAGHVIDN
                     AAQFLS"
BASE COUNT          287 a          774 c          697 g          257 t
ORIGIN      
        1 atctgcccgc ccgaggcgat cgcgctgggc gacgcgatcg tcgcgcacat ggagccgacg
       61 atcgcggcgt cgccggcggc gatccagcgc gggctggcga tcggcctggt gctctatgac
      121 ctgggcgcgc tgccgcggca cgcccggcgg gcccgcgcgc tgaccggcga ccgcgccgag
      181 cgctactacg agcgctggct ggatggcccg acggcgatcc accgccagct cgcgcgcggc
      241 gtcaagcagc tgatgagcct gtcgtgctac gagcagcccg agatgatgga ccgcgtcggc
      301 taccggcccg cgccatgggt cgccgaggtc acccagcgcc ggctcacggt ctaccaggac
      361 gatgtgcagc gccaggccga gcagctgatc gcgcccgatc cgctgcggcc cggcgtccgg
      421 gtcaggcccg ggaaggagcc cagctgatgg tcgccgcccg gcccgccgtc cacgagggcg
      481 cgccgccgcc cggcgtgttc acccgcaacg accaccacgg cgacagcgtg ctcgacgccg
      541 acgccgtgat cgtcggctcg ggcgccggcg gcgcgacgat ggccgccgag ctcgccgagg
      601 ccgggttcga cgtcgtcgtg ctcgaggagg gcagctacta ccagacccgc gacttcaccg
      661 ccgacagctc ggcgatggcg cgccagctct accgcgacgg cggcgcgacc gccgcgctcg
      721 gcgacccgcc gatcctctac caggagggca gcgcggtcgg cggctcgacc gtgatcaacg
      781 gcgggatgtc gtggcgcacc cccgacaaga tcctcgcgcg ctgggagcgc gagctcggcc
      841 tggccggcat cgcgcgcgac ctcgagccct acttcgcccg ggtcgagcgc cggatccacg
      901 tcgcgaagat ggatcccgac gtgatcggca ccgacaactg gctgctcaag aagggcgccg
      961 acgccaaggg ctggaaggtg atcgacaacc tgcgcaacca ggtccactgc gtcggctcca
     1021 accgctgcgc gttcggctgc ccgaccggcg ccaagcagag cgcgctggtc agctacatcc
     1081 cacgcgcgct gcactacggc gcgcgggtct acgccgacgt ccgggtcgat cgcatcacgc
     1141 gccacggcaa gcgcgcgacc ggcgtggtcg gcacggtcaa gggcaccggc caccggatca
     1201 ccgtgcgggc gcggctggtg gtcgcggcgt gcggcgcgat ccacaccccg gcgctgctgg
     1261 tgcgctcggg gttccgctcg ccgtcgggcc agctcggcca caacctgtcg atgcacccca
     1321 acgtcaaggt cgtcgcgatc ttcgaccagc cgatcaccgg ctggaagggc acccaccagg
     1381 cgttccaggt ccgcgagttc caggacgagg gcctgctgtt cgccgcggtc aacctgccgc
     1441 cgtcgatcct ggcgatgtcg ttcccgcacc gcggcgccgc gctcggcgag ctgatggacc
     1501 agtacgacca catggtgacc gccggcatgc tgtgcgagga caccacgacc ggccgggtcc
     1561 gcacgatcga cggcaagccg caggcgttct accagctcgc cggcgccgac gccgcgaacc
     1621 tcaagcgcgg cgtggtgctc ttgtccgagc tgctgttcgc cgccggcgcc cgccggatcc
     1681 tgctgccgtt ccacggcgcg cgcgacctgt actcgcccga cgacgcgcgc cgcctgctcg
     1741 accagcccat cccgatcaag ggctgggagg tcgtcaccgt ccacatgatg ggcaccgcgc
     1801 ggatgggcgc cgatcgcagc cacgcggtca ccagcccgtt cggcatggtg cacgacgccg
     1861 accggctgat gatcgccgac gcctcgctgt tcccgacccc gatcggcgtc aacccgatgg
     1921 agacgatcat ggcgctcgcg acccgcggcg ccggccacgt gatcgacaac gccgcccagt
     1981 tcctgtcatg acccccggcc tgccgtccgc cgccg
//
LOCUS       DASVOR010002068         2462 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_283918,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002068 DASVOR010000000
VERSION     DASVOR010002068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2462
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_283918"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            335..>2462
                     /locus_tag="VF516_38450"
     CDS             335..>2462
                     /locus_tag="VF516_38450"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEX8113695.1"
                     /translation="MGASLCGCGSTSGGTAPSLTITPSGTSGTVSITGPTDFTAQLVG
                     STDEVAWTVTGGTLTAMTGLHVVYMPPPGSAMGMLTATAGNLTAMVKITSSPVALSGK
                     KIPGLTAQVTVQYDAQDVPHIQCAQAEDCIAVQGYLQARDRLFPMDFLRHVAEGHLAE
                     LIGPAGLSSDLQLRTVLLTRDGRPVQSALKDALDAKTLALMNKFVAGVNAYINELKTT
                     TGAALPGEYAQLPFPLKPADIAAWTIEDTLAIGRLNQFQLSETLNGELANGKFALTYG
                     PGAPHQDLGKMNAWIRAAAPTSEQAHTLSPTGSHVSTAAAPVIPASIRGPRANLRADL
                     AKWRAPLNALAGRIAALRDRLRPLDASVGSNNWVVSAAKSATGAAMVANDPHLSLQYP
                     PLFHLAAMTSANAADNLNVTGGSFPGVPGALVGRGAHVGWGVTVGGYDVTDVYMEQFL
                     PPNSCQSAAPCVLFKGGPVATIPVPETFMVRTPGGLVDAATLPAAALPVTIPPFLLVV
                     PHHGPIVQPPDASGHALSFRWTGHEGTTQDSKAFYGLNTATDVDAAMTALNDYATGAQ
                     NFVLADDHGNIAYYPHALVPIRNFADARITAPDKLIPPWFPLPGDGSAEWGDGTSACA
                     SPTAGQIASCWTANTDLPQGKNPAKGYFFTANADPTSLPTNPEKGVSDDNSPLAHPPY
                     MSFDWDDSSGFRATRIEQMMKDALAAN"
BASE COUNT          375 a          910 c          840 g          337 t
ORIGIN      
        1 ccggcgcgcg gcgcggtcac cggggctgcc cgcggcgcgg gtcgctcgag cacgccctac
       61 gataccgcgc gcggcggtcc ggcgccgtcg ctgccggctc ctgccagcgg ccgcagcgag
      121 gccccgacgc cgcaatggcc gggacgctgt gatgcgtcat gaacgagtct cgaggggttc
      181 tccgctcctc ggccaacaca gcgccgcgtt cctttggtaa agggttaggg tcctcgggcc
      241 ggtacgcgat gtatacgcgt gcgaccggtc cttttcgacc tcgttagcct tgggagaatt
      301 catgcgctcg ttgctcgtcg cggcagcttg gctcttggga gcatcgcttt gcggatgtgg
      361 gtcgaccagc ggcggtacag ccccatccct cacgatcacc ccgtcgggca catcgggcac
      421 cgtctcgatc accggcccga ccgacttcac cgcgcagctc gtcggcagca cggacgaggt
      481 cgcgtggacg gtcaccggcg gcacgctgac ggccatgacc gggctccacg tcgtctacat
      541 gcccccaccg ggcagcgcga tggggatgct gacggccacg gccgggaacc tgacggcgat
      601 ggtgaagatc acctcgtcgc ccgtggcgct gtcgggcaag aagatcccgg ggctgaccgc
      661 gcaggtgacg gtgcagtacg acgcccagga cgtcccgcac atccagtgcg cccaggccga
      721 agattgcatc gccgtccagg gctacctcca ggcccgcgac cggctgttcc cgatggactt
      781 cctgcgccac gtggcggagg gccacctcgc cgagctgatc ggccccgccg ggctgtcgtc
      841 ggacctccag ctgcggacgg tgctcctgac gcgagacggc cggccggttc agagcgcgct
      901 caaggacgcg ctcgacgcca agacgctggc cctgatgaac aagttcgtcg ccggcgtgaa
      961 cgcgtacatc aacgagctca agaccacgac cggcgcggcg ctgcccggcg agtacgcgca
     1021 gctgccgttc ccgctgaagc cggccgacat cgcggcgtgg acgatcgagg atacgctggc
     1081 gatcggccgg ctcaaccagt tccagctgtc ggagacgctg aacggggagc tcgccaacgg
     1141 caagttcgcg ctgacctacg gcccgggcgc gccgcaccag gatctgggga agatgaacgc
     1201 gtggatccgg gcggcggcgc cgaccagcga gcaggcccac acgctgtcgc cgaccgggtc
     1261 gcacgtctcg accgccgcgg cgccggtcat cccggcgagc atccgcgggc cgcgggcgaa
     1321 cctgcgggcg gacctcgcga agtggcgcgc gccgctcaac gcgctggccg gccggatcgc
     1381 cgcgctgcgc gatcggctgc gcccgctcga cgccagcgtc ggctccaata actgggtggt
     1441 gtccgcggcg aagtccgcca ccggcgccgc gatggtcgcc aatgacccgc acctgtcgct
     1501 gcagtacccg ccgctgttcc acctcgcggc gatgacgtcg gccaacgcgg ccgacaacct
     1561 caacgtgacc ggtggctcgt tccccggcgt cccgggggcc ctggtcggcc gcggtgcgca
     1621 cgtcggctgg ggcgtgaccg tcggcggcta cgacgtgacc gacgtgtaca tggagcagtt
     1681 cctgccgccg aacagctgcc agagcgcggc gccgtgcgtc ctgttcaagg gggggccggt
     1741 ggccacgatc ccggtgcccg agacgttcat ggtccgcacg ccgggggggc tggtcgatgc
     1801 cgcgacgctg ccggccgccg cgctcccggt gaccatcccg ccgttcttgc tggtcgttcc
     1861 gcaccacggg cccatcgtcc agccgcccga cgccagcggg cacgcgctga gcttccggtg
     1921 gacgggccac gagggcacca cccaggacag caaggcgttc tacggtctca acaccgcgac
     1981 cgacgtcgac gccgcgatga cggcgctcaa cgactacgcc accggcgcgc agaacttcgt
     2041 gctcgccgac gaccacggca acatcgccta ctacccgcac gcgctggtgc cgatccgtaa
     2101 cttcgccgac gcgcgcatca ccgccccgga caagctcatt ccaccgtggt tcccgctgcc
     2161 cggcgatgga tcggccgagt ggggcgacgg cacgagcgcc tgcgccagcc cgaccgcggg
     2221 ccagatcgcg tcgtgctgga ccgccaacac ggacctgccg cagggcaaga accccgcgaa
     2281 gggctacttc ttcaccgcca acgcggatcc gacgtcgctg cccaccaacc cggagaaggg
     2341 cgtgtccgac gacaacagcc cgctcgccca cccgccgtac atgagcttcg actgggacga
     2401 ctccagcggg ttccgcgcca cgcggatcga gcagatgatg aaggacgcgc tcgctgcgaa
     2461 cg
//
LOCUS       DASVOR010002069         2255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401116,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002069 DASVOR010000000
VERSION     DASVOR010002069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401116"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1210
                     /locus_tag="VF516_38455"
     CDS             <1..1210
                     /locus_tag="VF516_38455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113696.1"
                     /translation="LPADPSKANKPPPTPHAEAKPADAKLAKPHDDHHDAPKAASGPV
                     QSHHPAHGYDRDDKTGLDSFGNLTERTKRTEKNMTTGHKRVFTNQFTTVSKAPLVTHE
                     LKNAHRTLDKGTVLHTYDVNGQYAQVVAPGITIEDNLWINFQYIGGNGSTQPEFGNEH
                     EDAADKARADAIRAGLPKTGRNPGKSKHTWQFTSAFLPSSEGIALDGSLMSKVVKLME
                     WAIAEDMVTGDIQIKSGVRPPITAHKMCIRFELAYRDGKNVDFDAIRKLPDGMYHGWK
                     FCPSPGKSSDEEIKKQAHELYKTEGGSGAQAAAGFAPGTPGRSPLKDTGPGVSKHCPG
                     HAVDISIPWRSPENPEATDLWAWEQIYHQFGLTRPLHRDRGFSGHDAEHWHIEETGKQ
                     IAGIEKEGGD"
     gene            1207..1902
                     /locus_tag="VF516_38460"
     CDS             1207..1902
                     /locus_tag="VF516_38460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113697.1"
                     /translation="MKTAACVLAIACAMGPAVADPKKDRAAVAQLEAMYEPIGKLSGA
                     DRVNRACGDAVKLRDASNAFSDEKAPAGAVVDDQGWARAARALEGALNALVAVCKAPD
                     HKRKLLDEVETADQVVVTVDDDMRALFELVKPRMLPAAVKTFRTTVAAIRFPSKSFCA
                     HVAKLTRQVGGLATPPPRADAAKWQQAFTAVKTSVENLQCTRPPAADEQIAGGLAGLH
                     DELDALVLLVPPS"
     gene            complement(1917..>2255)
                     /locus_tag="VF516_38465"
     CDS             complement(1917..>2255)
                     /locus_tag="VF516_38465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113698.1"
                     /translation="AYNKAAFLADPLCMQVADKIRECVGKPEFIAALDSGATAQQKKT
                     NARLRKGVKKWQSSYELCHNAWDLLNYEYTGFLDKPAVFKAPNALASCAELGAAVKAG
                     GGLVGGKTAE"
BASE COUNT          408 a          803 c          750 g          294 t
ORIGIN      
        1 cctgccggcc gacccatcca aggcgaacaa gcccccgccc acgccgcacg ccgaggccaa
       61 gccggccgac gccaagctcg ccaagccgca cgacgatcac cacgacgcgc ccaaggccgc
      121 gtcggggccg gtgcagtcgc accacccggc gcacggctac gaccgcgacg acaagaccgg
      181 cctggactcg ttcggcaacc tgaccgagcg caccaagcgc accgagaaga acatgaccac
      241 cggccacaag cgggtgttca ccaaccagtt cacgacggtg agcaaggcgc cgctggtgac
      301 tcacgagctc aagaacgcgc accgcaccct cgacaagggc accgtgctgc acacctacga
      361 cgtcaacggc cagtacgccc aggtcgtcgc cccgggcatc acgatcgaag acaacctgtg
      421 gatcaacttc cagtacatcg gcggcaatgg ctcgacccag ccggagttcg gcaacgagca
      481 cgaggacgcc gccgacaagg cgcgcgccga cgcgatccgc gccggcctgc ccaagaccgg
      541 ccgcaacccg ggcaagagca agcacacgtg gcagttcacc agcgcgttcc tgccgtcgtc
      601 cgagggcatc gcgctcgatg gctcgctgat gtcgaaggtc gtcaagctga tggagtgggc
      661 gatcgccgag gacatggtca ccggcgacat ccagatcaag tccggcgtgc gcccgccgat
      721 caccgcccac aagatgtgca tccggttcga gctggcgtac cgcgacggca agaacgtcga
      781 cttcgacgcg atccgcaagc tgcccgacgg catgtaccac ggctggaagt tctgcccgag
      841 ccccggcaag tcgtccgacg aggagatcaa gaagcaggcc cacgagctct acaagaccga
      901 aggcggcagc ggcgcgcagg cggcggcggg ctttgccccc ggtactcccg gtcgctcacc
      961 gctcaaggac accggcccgg gcgtgtcgaa gcactgcccc ggccacgcgg tcgacatctc
     1021 gatcccgtgg cgcagcccgg agaaccccga ggccaccgat ctgtgggcct gggagcagat
     1081 ctatcatcag ttcggattga cccggccgct gcacagggat cgcgggttca gcgggcacga
     1141 cgccgagcac tggcacatcg aggagaccgg caagcagatc gccggcatcg agaaggaagg
     1201 tggcgattga agacagccgc gtgtgtcctg gcgatcgcct gtgcgatggg tccggcggtc
     1261 gccgatccga agaaggatcg cgccgcggtc gctcagctcg aggcgatgta cgagccgatc
     1321 ggcaagctgt cgggtgccga ccgggtcaac cgcgcgtgcg gtgacgcggt caagctgcgc
     1381 gacgcgagca acgcgttctc cgacgagaag gctcccgcag gcgcggtggt cgacgaccag
     1441 ggctgggccc gggcggcgcg cgccctcgag ggtgccctca acgcgctggt cgccgtgtgc
     1501 aaggcgcccg atcacaagcg caagctgctc gacgaggtcg agaccgccga ccaggtcgtg
     1561 gtgacggtcg acgacgacat gcgcgcgctg ttcgagctcg tgaagccgcg catgctgccg
     1621 gccgcggtca agacgttcag gaccacggtc gccgcgatcc ggtttccgag caagtcgttc
     1681 tgcgcgcacg tcgcgaagct gaccaggcag gtcggcggcc tggcgacgcc gcccccgcgc
     1741 gccgacgcgg cgaagtggca gcaagccttc accgccgtca agaccagcgt cgagaacctc
     1801 cagtgcacca ggccacctgc cgccgacgag cagatcgcgg gcggcctcgc cggtctgcac
     1861 gacgagctgg atgcgctcgt cctgctcgtg ccgccgagct gaccgtcagc cggccgtcac
     1921 tccgccgtct tgccgccgac caggccgccg cccgccttga cggcggcgcc gagctccgcg
     1981 cacgacgcca gcgcgttcgg ggccttgaac accgccggct tgtcgaggaa gccggtgtac
     2041 tcgtagttca ggaggtccca ggcgttgtgg cacagctcgt acgacgactg ccacttcttc
     2101 acgcccttgc ggaggcgggc gttggtcttc ttctgctgcg cggtcgcgcc gctgtcgagc
     2161 gcagcgatga actcgggctt gccgacgcac tcgcggatct tgtcggcgac ctgcatgcac
     2221 aacggatctg ccaggaacgc cgccttgttg taggc
//
LOCUS       DASVOR010002070         2161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_167825,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002070 DASVOR010000000
VERSION     DASVOR010002070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167825"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(342..1874)
                     /locus_tag="VF516_38470"
     CDS             complement(342..1874)
                     /locus_tag="VF516_38470"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HEX8113699.1"
                     /translation="MKTALIVLALLAPAVARADVTRHALIVGHDRGHPDEQTLRYAED
                     DARRLAEVLVDLGGFAADHVTVLRGPAPDAVLDALIAINDRIRTRGEPAVLVVFYSGH
                     ADAQALHLGAGELSLERLEQLVRGSAAAFRLLIVDACRSGALTQVKGGAPVPAQPVQL
                     AERLPADGLVVWTSSSATEDAQESDDIQGSFFTHYLVSGLLGAADRNGDGTVSLGEAY
                     AHAYDATLRASSRTLAGVQHATFREDVHGLADLTLTWLDGRARGLVAFPPGHDYLVMM
                     GGPDGPVVAEVGARDAERRVSLRVGRYFVRARTADALLEGTVAVRAGERVAVDEGALK
                     RSDYARLVRKGAGGRARADRVELGYTIRSSLYADAGPCQGATAGWGVDLPAFSLGLRA
                     VACRGGATNAFVDATHDQLAAEARAVHAWDVDHVTLAIGPLAGTALLVEHFTGGASTR
                     TSLAPLAGVTAGASLDLADRLYLTGEVDAVTTFFRQEQMGEASLHAAFAVRGNLLVGM
                     RW"
     gene            complement(1871..>2161)
                     /locus_tag="VF516_38475"
     CDS             complement(1871..>2161)
                     /locus_tag="VF516_38475"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEX8113700.1"
                     /translation="EVVHPGDTLSYLVTTAEPAYVTVLGRDAAGRVTTYVPAAPVAAG
                     RDVELPIATLLDGTLGREDIVAVFCAGAMPFPSIDDPPAGCTVDRLAIEKTP"
BASE COUNT          308 a          789 c          800 g          264 t
ORIGIN      
        1 cctggttgcc cgacacctcg gcgaccggcg ccttggcgtc cacatcgctc gcgtcgttcg
       61 cgtcgctggc gctgtccgta caaccggcgg cggtccagag caccccgagc gagagaactg
      121 cgagcgtctt catgccgggg ccatagagca tgcgccaggc caagttttgc gcaccgtaat
      181 tgctcgaaat aactcaacta agcccctggt ccacgctggt accagcggtg taccagggag
      241 cacctccgga ggtccagggg gcaatgtgcc cctcgcaaga cgcgcgcgat ccctgcctcc
      301 gtgaggtgcc ccgtacacgc gcgcggcggc ggcctgcgcc gctaccagcg catgccgacc
      361 agcaggttgc cgcgcaccgc gaacgccgcg tgtagcgacg cttcgcccat ctgctcctgg
      421 cggaagaacg tcgtgaccgc gtcgacctcg ccggtgagat acaggcgatc ggcgagatcg
      481 agcgacgcgc ccgccgtgac ccccgcgagc ggcgcgagcg aggtgcgcgt gcttgcgccg
      541 ccggtgaagt gctcgacgag cagggccgtg ccggcgagcg ggccgatcgc gagcgtgacg
      601 tggtcgacat cccacgcgtg caccgcgcgg gcctcggccg cgagctggtc gtgcgttgcg
      661 tcgacgaacg cgttggtcgc gccgccccgg caggccaccg cgcgaaggcc gagcgagaac
      721 gccggcagat ccacgcccca gccggcggtc gcgccctggc acgggccggc gtcggcgtac
      781 agcgacgatc ggatcgtgta accgagctcg acgcgatcgg cgcgcgcgcg gccgcccgcg
      841 cccttgcgca cgagccgtgc gtagtcgctg cgcttgagcg cgccctcgtc gaccgcgacc
      901 cgctcgccgg cgcggaccgc gaccgtgccc tcgagcaggg cgtcggccgt gcgcgcgcgg
      961 acgaagtagc gaccgacgcg caggctgacc cggcgctcgg cgtcccgcgc accgacctcg
     1021 gcgacgaccg ggccgtccgg accgcccatc atgaccagat agtcgtgacc gggcgggaac
     1081 gcgacgaggc cgcgcgcgcg gccgtcgagc caggtcagcg tgagatcggc gaggccgtgg
     1141 acgtcctcgc ggaacgtcgc gtgctgcacg ccggcgagcg tgcggctgga cgcgcgcagg
     1201 gtggcgtcgt aggcgtgcgc gtaggcctcg ccgagcgaga ccgtgccgtc gccgttgcgg
     1261 tcggccgcgc cgagcaggcc cgacaccagg tagtgggtga agaacgagcc ctggatgtcg
     1321 tcggactcct gggcgtcctc ggtcgccgag ctcgacgtcc acacgacgag accgtcggca
     1381 ggcagccgct cggcgagctg caccggctgg gccggcaccg gcgcaccgcc cttgacctgg
     1441 gtcagcgcgc cggagcgaca tgcatcgacg atcagcagcc ggaacgccgc cgccgagccg
     1501 cgcacgagct gctcgagccg ctcgagcgac agctcgccgg cgccgaggtg cagcgcctgc
     1561 gcatcggcgt gcccggagta gaacacgacg agcaccgcgg gctcgccgcg cgtccggatc
     1621 cggtcgttga tcgcgatcag cgcgtcgagc accgcgtccg gcgccggccc gcgcagcacg
     1681 gtcacgtggt ccgcggcgaa tccgcccaga tcgacgagga cctcggcgag ccggcgggca
     1741 tcgtcctcgg catagcgcag cgtctgctcg tcgggatggc cgcggtcgtg tccgacgatc
     1801 agcgcgtggc gcgtgacgtc ggcgcgcgcg accgccggcg cgagcaacgc gagcacgatc
     1861 agcgccgtct tcacggcgtc ttctcgatgg cgaggcgatc gaccgtgcag cccgcaggcg
     1921 gatcgtcgat cgaggggaac ggcatcgcgc cggcgcagaa cacggcgacg atgtcctcgc
     1981 ggccgagcgt gccgtcgagc agcgtcgcga tcggcagctc gacgtcgcgc ccggcggcga
     2041 cgggggccgc cggcacgtac gtcgtgaccc ggcccgcggc atcgcggccg agcacggtga
     2101 cgtacgcggg ctcggcggtc gtcaccaggt agctcagcgt gtcgccgggg tggacgacct
     2161 c
//
LOCUS       DASVOR010002071         6743 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_51661,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002071 DASVOR010000000
VERSION     DASVOR010002071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6743
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51661"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VF516_38480"
     CDS             complement(<1..283)
                     /locus_tag="VF516_38480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113701.1"
                     /translation="MTASNLTVVTPLAASPEAKAADPATAWPGQAAIVPAAMVAPAVA
                     DDPVPSEVSGAIELFATQLSKVSFPEVDAASLRRAADELRAEARAVARAR"
     gene            complement(459..608)
                     /locus_tag="VF516_38485"
     CDS             complement(459..608)
                     /locus_tag="VF516_38485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113702.1"
                     /translation="MLVLTKVLFMREAAERKIWTSSLVWPTHTARSFGAPGRPFPPAC
                     AHGAG"
     gene            1888..3618
                     /gene="glsA"
                     /locus_tag="VF516_38490"
     CDS             1888..3618
                     /gene="glsA"
                     /locus_tag="VF516_38490"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR03814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEX8113703.1"
                     /translation="MTGKHTEGHFNAGGQTTTIAITPTMGAVGVDSQAKLPEDAEIDD
                     LRERRLFISLDRDRKGHVTRQEITRALARAGLDAKDSRLRECMVGLTGLGDSDLLEYD
                     QFCRIIRPNILLIEQALQGTLVIPDFEEFAHEIEQIYTETRTACDGDLARYIHQLAVV
                     DPEQYAIALCTVDGQRYETGDSHTDFCVQSCCKPINYCLALEECGEEVVHSHVGREPS
                     GHGFNEIALNAAQKPHNPMINAGAIMCCSLIRQDADMAGRFEHVMERWKSLCGDDKVR
                     FSNSVYLSERATADRNFALGYFLREKQAFPANTDLVATLEFYFQCCSIEATAAQLSIA
                     AATLANGGICPTTGKRIFQPKTVQHCLSLMYSCGMYDFSGEFAFTIGLPAKSGVSGAL
                     MIVVPNVMGICTWSPRIDNHGNSVRGLAFCRKLVEKFRFHNYDNLRGLHEKHDPRQPR
                     IRVDADVVMALIWAASKGDLSAIQRLVACGADLNGADYDGRTPLHLAASEGHEDVVRY
                     FIDQGVNINPVDRWGGTPLCDAQRHGHEVVMELLERSGGTAIINRSASVDRPIKQALE
                     PRREAWLRKE"
     gene            3651..5072
                     /locus_tag="VF516_38495"
     CDS             3651..5072
                     /locus_tag="VF516_38495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEX8113704.1"
                     /translation="MHYRTLIPFVMLVALGVIGLLAPISIPAASGPPVNSGDTAWMLT
                     AAGLVLLMTPGLSFFYGGMVRTKNVLSTMLQSFIAMAVISIIWVVVGFSLVFGDSIGG
                     LIGNPTTYYMFRGVGGATNAALSPTIPLVLFALFQLKFAIITPALVSGAFAERIRFSS
                     YIIFISLFTIVIYCPLAHATWHPDGLLHKWGVLDFAGGTVVHMSAGLAALAGAFVIGP
                     RSDHKHRQHTPPANLPYVLLGTGLLWFGWFGFNAGSALSASPLASHAFMTTNTASASA
                     MLSWMLFDKVRGLKPSALGSCTGAVVGLVAITPAAGYVSVGASIFIGVAASVMSNLVA
                     YRKSKSALDDTLDVFPCHGVGGMFGMICTGLFANTTINANCTNGLMYGGTTLFLHHAV
                     SLIAVTVLVFSISCLLYKVTNWISPLRVDAEQERIGLDISQHGESVAVEAHSGQLGHL
                     EPALATDAHSDGETLEKQSVAWG"
     gene            5130..5477
                     /locus_tag="VF516_38500"
     CDS             5130..5477
                     /locus_tag="VF516_38500"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEX8113705.1"
                     /translation="MDLGQTSGIWPTVARDLPAGIERLATGVVDAGGGQLSAHDLGAL
                     AAHPAGRDLLAAVSCGSVSMGALTYIGSGPNFVVKAIAEQHHVRMPGLFGYLVCSVGT
                     LVPVLVAVSALVF"
     gene            5489..6310
                     /locus_tag="VF516_38505"
     CDS             5489..6310
                     /locus_tag="VF516_38505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8113706.1"
                     /translation="MVCLESSLSTARAVDVAIELARSLPAGLVGLAVVDEPDISAGAA
                     TNIGGASDRCDRDAALLEDTHARARAWLADFVGCGRAAGVAVRTLQIAGSPSEAILAE
                     GPRHDLTVLGRDVQFRYETQEHDRYSRDRILRCTGRPVLLVPDQIAATGSAVMIGYDG
                     SPAAIRALHSFADSQLARGRAIHVATVDDDGAHAFETATRGCGLLAGLGIRAVPENVA
                     SSESTAAALLSCAARLGAGMIVIGGYIPSPLARVVWGSVTHEVIARATVPVFLHY"
     gene            6381..>6743
                     /locus_tag="VF516_38510"
     CDS             6381..>6743
                     /locus_tag="VF516_38510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113707.1"
                     /translation="MSYDDDLDARQAAAEEAMDTDCAASVMGGGPKSGVFPKPLNPNV
                     EVPGRPVDDPMPEYEPEPPEMPPDPGPPPSSPPEPLQPPETYPDVEPGAPDAPQVDPT
                     PGEGAVEGAEGTELAEGAE"
BASE COUNT         1207 a         2175 c         2110 g         1251 t
ORIGIN      
        1 cgcgcgcccg cgccaccgcc cgggcctcgg cgcgcagctc gtcggcggcc cgccgcagcg
       61 acgccgcgtc gacctcgggg aacgagacct tggacagctg ggtggcgaac agctcgatcg
      121 cgccgctgac ctcgctgggc accggatcgt cggcgactgc gggggccacc atcgccgctg
      181 gaacgatcgc cgcctgcccg ggccatgcgg tggcgggatc ggcggccttg gcctccggcg
      241 aggctgcgag aggagtcacc acggtcaggt tggatgccgt catgaacatc tgttcagtac
      301 acgccatcgc acgggttcgt caactcccgg aacctaaatc cttccaagag cgaacgatct
      361 ccactcgtac gcgctcatcc cagcgccggt ttgccacccg gagcgcagaa gctcaccgcc
      421 gggtgcgcat gcctcgagga ggacgagcct cgatgcgatc agccggcgcc gtgagcgcaa
      481 gccggtggga acggtcggcc tggcgcgccg aacgaccggg cggtatgcgt cggccacacc
      541 aggctgctgg tccagatctt gcgctcggcc gcttcgcgca tgaacagcac cttggtcagc
      601 acgagcacgt acggaccctg cctcgccggg tcatgtgggt gaccggaagc tcgtgagggt
      661 gatcgtctgg tggacgtggg cgcggcggtc tttggcgagc ggtcagtcga ccgctgctca
      721 tccgcgtggc gctcggtcgc tttctggagg atggcaacat cccaatagac aacggcgctg
      781 tcgagagact tcatattcgc gcagcgccga ccagaaaaac ttcctattcg ctgggtgcga
      841 attctagtga agatcgcgcg tcgatcgtat tcatgatctt cggctcagat cgactcgctg
      901 gaatcgatcc cctcgaacac ctccctgaca ttctgccgcg ccttacccat tcgagccgga
      961 tgtccgagat ctcgggatgc ttcccgtggc tggaagattc aggcactgcc gcaatttgcc
     1021 gaactccttc cccagggcct ttgctcggac ggacgcgcac gtgagggccc aggcgccaac
     1081 ctggcggacc ttcgcacccc aacgcggtgt ctccgtcgga catccgcgag aatttcatac
     1141 atgatggagg ctcgcggttg aacgtacatc tcgacgagac ctgcaccagt cgcccccggc
     1201 gatgattgac tgtgcgagcg ggctatcggg atcgcctctg aggagacggc gaggtcttgg
     1261 ggggcatcca ccgggggctc catcatgtat aacattgccc gcgttctcgt cgggaaagcg
     1321 ccttcattct gcgaaggtcc cactagaccc tcccctcgtt tcatcgcggc tcggacctac
     1381 agcaaccgac ctggccgcgt cactcctggc aactctccga cgagaaccag atggttccct
     1441 gggtatcggc gaattccggc agagacggtc ccaacggagg ccggtggtga cggatcaggg
     1501 gatcttatcc atcagtccgg actgcagccc atcagtgagc acgatggaga ccgacctgcc
     1561 gaccatcagc cagcccgccc tgaaacctgc gggcagccat caaaggacgt gctccctgca
     1621 atggagcaac tccccgacaa cggtgatggc aacctctttt atatcgccca tgcagtcgga
     1681 gcacggtgtc cccacggcgc tcatcgacca ttgggattta tgggacatgc atgggcatta
     1741 ccgatgcatg catcgcggcc ccatgaatgt tgtcgaaccc agcggttttt gttgctccag
     1801 cagatccgta ctcgaaaacg ttctacttca gactcgatgt ggcctcggcg aggctccgca
     1861 acccatcgcc aggatcgagc tggtaccgtg accgggaagc acaccgaggg gcatttcaat
     1921 gcgggagggc agaccacgac catagcgatc acgcccacca tgggggctgt cggggtggac
     1981 agtcaggcaa agcttccaga agatgcagag attgatgacc tgcgagagcg gaggctattt
     2041 atctcgctgg atcgcgatcg caagggacat gtcactcgac aagagatcac acgggcgctc
     2101 gcgcgggcag gtctcgatgc caaggatagc cggctacgcg agtgcatggt gggcctcacc
     2161 ggtcttggcg attcggatct cctcgaatat gaccaattct gccggatcat ccggccgaac
     2221 atccttctta tcgagcaggc actccaaggc acccttgtca ttccggactt cgaggagttc
     2281 gctcatgaga tcgagcagat ctacacggag acacgtaccg catgcgacgg cgatcttgcg
     2341 cgatacattc accagctggc tgtcgtagat cccgagcagt acgctatcgc actttgcacc
     2401 gtcgacggac agcgatatga aacgggcgac tcgcacacgg acttctgcgt ccagtcgtgc
     2461 tgcaaaccga tcaactactg tttggccctc gaggagtgtg gcgaggaggt cgtacacagc
     2521 cacgttggac gtgaaccgag cggacatgga ttcaacgaga tcgcgttgaa cgctgcgcag
     2581 aagccgcaca atcccatgat caacgccggg gcgatcatgt gctgctccct catccgacaa
     2641 gacgccgaca tggccggacg cttcgagcat gtcatggagc gatggaagtc gctttgcggg
     2701 gacgacaagg tgcgcttcag taactccgtc tatctctcgg agagggccac agcggaccgt
     2761 aattttgcac tcggatactt cttacgcgag aaacaggcat tccctgcaaa caccgatctt
     2821 gttgccacgc tggagtttta ttttcaatgc tgctctattg aagcaaccgc agcacaactc
     2881 tcgatcgcag cggctaccct tgccaatggg ggaatctgtc cgacgacggg gaagcgtatc
     2941 ttccaaccaa agacagttca gcactgcctt tcgctgatgt actcctgcgg aatgtacgac
     3001 ttctcggggg agttcgcctt caccatcggc ctcccagcca aaagcggcgt ttcgggtgct
     3061 ctgatgatcg tcgtaccgaa cgtcatgggg atctgtactt ggtcaccccg aatcgacaat
     3121 catgggaata gcgtccgagg gctggcgttc tgtcgcaagc tggtcgagaa gttccggttc
     3181 cacaactatg acaacctgag gggcctgcac gagaagcacg atccacgtca gccgaggatc
     3241 cgcgtggatg cggatgtcgt gatggcgctt atttgggcgg cgagcaaggg cgatctctcg
     3301 gcgatccaga ggctcgtggc ctgtggcgca gacctgaacg gcgcggatta cgacggccgt
     3361 accccgctcc acctcgccgc atccgaaggg catgaggacg tggtgcgcta cttcatcgac
     3421 caaggtgtga acatcaaccc ggtggatcgc tggggaggca cgccgctctg cgacgctcaa
     3481 cgtcatggtc acgaggtggt gatggagctg ctcgaacgat ccggaggaac ggctatcatc
     3541 aaccggtctg ccagcgtaga tcgtcccata aaacaagctc ttgagccacg acgtgaggca
     3601 tggctccgca aagaatgaga cccgctcgag gcgcagatcg gagcatcaga atgcattatc
     3661 gcacgttgat tcctttcgtc atgcttgtgg ccttgggcgt catcggcctg ctggcgccga
     3721 tctcgatacc ggcagcatcg ggacctccgg tcaactccgg ggataccgcg tggatgttga
     3781 ccgccgccgg gctcgtcctg ctcatgacac cggggctgtc cttcttctat ggcgggatgg
     3841 tgaggaccaa gaacgtcctt tcgaccatgc tccagagctt catcgcgatg gctgtgatca
     3901 gcatcatctg ggtggtggtc ggcttcagcc tcgtgttcgg tgacagtatc ggaggcctca
     3961 tcggaaatcc cacgacgtac tacatgttcc gaggcgttgg aggtgcaacg aacgcggcgc
     4021 tcagccccac cattccgctc gtgctgttcg cgctcttcca gctgaagttt gcgatcatca
     4081 cccctgcgct ggtgagcggt gcgtttgccg aacggattcg cttttcaagc tacatcatct
     4141 tcatctcact cttcactatc gtgatttatt gtccgcttgc acacgcgaca tggcacccag
     4201 acgggctgtt gcacaagtgg ggcgttctgg acttcgcggg cggcaccgtg gtccacatgt
     4261 cagcggggct tgcggcactc gcaggggctt tcgtgatcgg acctcggagc gatcacaagc
     4321 ataggcagca cactccgccc gcgaacctcc cctacgtcct acttggcacg ggcctgctgt
     4381 ggttcgggtg gttcggcttc aacgcgggtt cagcgctgtc cgcatccccg ctagcatcgc
     4441 atgccttcat gaccaccaac acggcttccg cttcggccat gttgtcttgg atgttgttcg
     4501 acaaggtgcg cgggctgaag ccttccgcgc tcgggtcgtg taccggagcg gtggtgggcc
     4561 ttgtggcaat cacccccgcg gccggctacg tcagcgtcgg cgcgagcatc ttcatcgggg
     4621 tcgcagcgag cgtcatgagt aatctggtcg cataccggaa gagcaagtca gcccttgatg
     4681 acaccctcga tgtcttcccg tgccacggtg tcgggggcat gttcgggatg atctgcacag
     4741 gcctcttcgc aaacactacg ataaatgcca attgtacaaa cggccttatg tacggtggaa
     4801 cgaccctctt tctacatcac gccgtctctc tgatcgccgt caccgtgttg gtattctcca
     4861 tttcatgtct cctgtacaag gtgacgaact ggatctcgcc gcttcgcgta gacgccgagc
     4921 aggaacgcat tggactcgac atcagtcagc acggcgaaag tgtcgccgtc gaggcacata
     4981 gtggccagct cggccatctc gagcctgcct tggcaacgga cgcacactcg gatggtgaga
     5041 cgcttgaaaa gcagtcagtc gcctggggct gacccctcat cggagttgat tccgagccgg
     5101 ttctgcaaca ccaactgacg agcaacctca tggatctagg acaaacaagc ggcatttggc
     5161 cgacggtagc tcgagacctc ccggcgggaa ttgagcggct cgcgaccggc gtggtcgacg
     5221 ccggcggggg acagctgtcg gcccacgacc tcggcgcact cgccgcgcat cccgccggcc
     5281 gcgacctgct ggcggcggtt tcgtgcggat cggtatccat gggcgcgctc acgtacatcg
     5341 gcagcggccc gaacttcgtg gtcaaggcga tcgccgagca gcaccacgtc cgcatgccgg
     5401 gcttgtttgg ttacctcgtg tgctcggtcg ggaccctcgt gcccgtgctg gtcgcagtgt
     5461 cggccctggt attctgatga tggagatctt ggtctgcctc gagtcctcgc tgagcaccgc
     5521 gcgtgcggtc gacgtcgcga tcgagctcgc gcgctcgctg cctgcgggtc tggtcggcct
     5581 ggcggtcgtc gatgagcccg acatctccgc cggcgcggcg accaacatcg gcggcgcgag
     5641 cgaccggtgc gaccgcgacg ccgcgctgct cgaggacacc cacgcccgcg cgcgggcctg
     5701 gctcgccgac tttgtcgggt gcggccgggc cgccggggtc gcggtccgca ccctgcagat
     5761 cgccggcagc ccgtccgagg cgatcctcgc cgaggggccg cgccacgatc tgaccgtgct
     5821 cggccgcgac gtccagttcc ggtacgagac acaggagcac gatcgctact cccgcgaccg
     5881 catcctgcgg tgcacgggca ggccggtcct gctggtcccg gatcagatcg ccgccacagg
     5941 ctccgcggtg atgatcggct acgacggcag ccccgcggcg atccgcgcgc tgcacagctt
     6001 cgctgacagc cagctggcgc gtggccgcgc gatccacgtc gccaccgtcg acgacgatgg
     6061 cgcccacgca ttcgagacgg cgactcgcgg ctgcgggctg cttgccgggc tcgggatccg
     6121 cgcggtcccc gagaacgtgg cgtcgagcga gtcgaccgcc gccgcgctgc tgtcgtgcgc
     6181 agcgcggctc ggcgccggca tgatcgtgat cggcggctac atcccgtcgc cgctcgcccg
     6241 ggtggtctgg ggctcggtca cccacgaggt catcgcgcgc gccacggtcc cggtattcct
     6301 gcactactag attgatgtaa atccggtcga agccgatcgg gctgtcacat ccgccgggcc
     6361 gccgactcta ccgcggcatg atgagctacg acgatgacct cgacgcgcgg caggccgcgg
     6421 cggaggaggc gatggacacc gactgcgcgg cgagcgtgat gggcggcggc cccaagagcg
     6481 gcgtgtttcc caagccgctg aaccccaacg tcgaggtgcc gggccggccg gtcgacgacc
     6541 cgatgccgga gtacgagccc gagccgcccg agatgccgcc cgatccgggc cccccgccgt
     6601 cgtcgccgcc cgagccgctc cagccgcccg agacctatcc ggatgtcgag cccggcgcgc
     6661 ccgacgcgcc ccaggtcgac ccgacgcccg gcgagggcgc ggtcgaaggc gccgagggca
     6721 ccgagctggc cgagggcgcg gag
//
LOCUS       DASVOR010002072         2781 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_518330,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002072 DASVOR010000000
VERSION     DASVOR010002072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518330"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1521
                     /locus_tag="VF516_38515"
     CDS             160..1521
                     /locus_tag="VF516_38515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113708.1"
                     /translation="MRLYAALFAVGLVGYGLVAWDRIASQSSDPHFVLQADAWLHGRA
                     AIEPPLKGDDWAQVETVALDDGTTASGRRLITRPAFHALDGRELPLTRLRQSLGKTAY
                     VSFPPFPAVIMLPGAALAGRAANDVLPTVLVAALILPLALLALRRLSRAGLSVRSLGD
                     DLWLVAALAFGSVLWFSAVQGKVWFTAHVVGVALALIYAWASIEAAHPVVAGLALGAA
                     ALTRTAMAFMVPLLVCEAWRMCGGALAWRADRRAMLRALARPLIRFAVPLAGFAIAGM
                     VFNQVRFGSPTEFGHSYLQVRQQALIEQYGLASHHYLARNLAVAFTLLPELLPYPPYV
                     RISGHGLALWFTTPVLVVLLWPRERGPLHRPLWLCVAAVALPSLLYQNSGWLQFGYRF
                     SLDYLVLLILLLAVGGRPLTRTVKALIIAGIVINLFGAVTFYRAWQFYRVDGTSYDVV
                     VAH"
     gene            complement(1438..2574)
                     /locus_tag="VF516_38520"
     CDS             complement(1438..2574)
                     /locus_tag="VF516_38520"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8113709.1"
                     /translation="MGNKTKKIAVVLAGAVAKGAFEAGALQALARTDVEILRIVAASS
                     GALTGTVLASAVAAGQLVEGADRIAEIWRDHARLREVFHLSVRDLVKRNGLSDQKQLM
                     ALLRRHVPLTPTAHPVSLRLVVAPLAGRDHEIVTRTETRTATSFEAVRDFHAGHFESQ
                     DGLDAVLSAAVASAAFPLVFAPVQLHRDGRCIDGGCVNNTPLKWALEGHIGAQIDAVV
                     VIATSVEHRKDPPGSLTGLRALTGHLAGMLLDERLYRDLREAEQVNRALDRLDQLVRD
                     KVITDEQLHRVKHALHWSGRRQVDIVTIRPDKPLDGGAFSGFFRRKKRVQYVNAGLGR
                     GLDVLAQARWPRRPEGPSVRHDDVVAGAVDPVELPGPVERDRPE"
BASE COUNT          333 a         1062 c          973 g          413 t
ORIGIN      
        1 caccgcggtc gcgtcgcgcc gggcggcgca cacctcccag ccggcgatcg cgctcaggag
       61 cagcgccggc gcagcggcga tcggcagcgg tctcgcggac aacgctggca agctaccacg
      121 cgcatcgccg ggtgaacccg ccgcgtgtaa ggttgcccgg tgcggctgta cgcagcgctg
      181 ttcgcggtgg gcctcgtggg ctacggcctc gtcgcgtggg accggatcgc gagccagtcg
      241 tcggatcccc acttcgtcct ccaggccgac gcctggctgc acggccgcgc cgcgatcgag
      301 ccgccgctca agggtgacga ctgggcccag gtcgagaccg tcgcgctcga cgacggcacc
      361 accgcgtccg gccgccggct gatcacccgc cccgcgttcc acgcgctcga cggccgcgag
      421 ctcccgctca cccggctccg gcagtcgctc ggcaagaccg cctacgtgtc gttcccgccg
      481 ttcccggccg tgatcatgct gccgggggcc gcgctcgccg gccgcgccgc caacgacgtg
      541 ctccccaccg tgctggtcgc ggcgctgatc ctgccgctcg cgctgctcgc gctgcgccgg
      601 ctgtcccgcg ccgggctgtc ggtccgctcg ctcggcgacg acctgtggct ggtcgccgcc
      661 ctggcgttcg gcagcgtgct gtggttctcg gcggtccagg gcaaggtctg gttcaccgcg
      721 cacgtcgtcg gcgtcgcgct cgcgctgatc tatgcctggg cgtcgatcga ggccgcgcac
      781 ccggtggtcg ccggcctcgc gctcggcgcc gccgcgctga cccggaccgc gatggcgttc
      841 atggtgccgc tcctggtctg cgaggcctgg cggatgtgcg gtggggccct ggcctggcgc
      901 gccgatcgcc gggcaatgct gcgcgcgctc gcccgcccgc tgatccggtt cgccgtcccg
      961 ctcgcggggt tcgcgatcgc cggcatggtg ttcaaccagg tgcggttcgg ctcgccgacc
     1021 gagttcggcc acagctacct ccaggtccgc cagcaggccc tgatcgagca gtacggcctg
     1081 gcgagccacc actacctcgc gcgcaacctc gccgtcgcgt tcaccctctt gcccgagctg
     1141 ctgccgtacc cgccctacgt ccggatcagc ggccacggcc tcgcgctgtg gttcaccacg
     1201 cccgtgctgg tggtgctgct gtggccgcgc gagcgaggcc cgctgcatcg cccgctgtgg
     1261 ctgtgcgtcg cggcggtcgc cctgccctcg ctgctgtacc agaacagcgg ctggctccag
     1321 ttcggctacc ggttcagcct cgactacctg gtcctgctga tcctgctgct cgcggtcggc
     1381 ggccggccgc tgacccgcac ggtcaaggcg ctgatcatcg ccggcatcgt gatcaaccta
     1441 ttcggggcgg tcacgttcta ccgggcctgg cagttctacc gggtcgacgg caccagctac
     1501 gacgtcgtcg tggcgcactg acggcccctc cggccggcgc ggccagcggg cctgggcgag
     1561 cacgtccagc ccccgaccga ggccggcgtt cacgtactgg acgcgcttct tgcgccggaa
     1621 gaacccgctg aacgcgccgc cgtccagcgg cttgtccgga cggatcgtga cgatgtcgac
     1681 ctggcgccgc cccgaccagt ggagcgcgtg cttgacccgg tgaagctgct cgtccgtgat
     1741 gaccttgtcc cgcacgagct ggtcgagccg gtcgagcgcc cggttgacct gctcggcctc
     1801 gcgcagatcg cggtacagcc gctcgtcgag cagcatcccg gcgaggtggc cggtcagcgc
     1861 gcgcagcccg gtcagcgagc cgggcgggtc cttgcgatgc tccaccgagg tggcgatcac
     1921 cacgaccgca tcgatctgag cgccgatgtg accttcgagc gcccacttca gcggggtgtt
     1981 gttcacgcag ccgccgtcga tgcacctccc gtcgcggtgg agctggacgg gagcgaacac
     2041 gagcgggaac gccgccgacg ccaccgcggc cgacagcacg gcatcgaggc cgtcctgcga
     2101 ttcgaagtgc ccggcgtgga agtcgcgcac ggcctcgaag ctcgtcgccg tgcgggtctc
     2161 ggtccgcgtc acgatctcgt gatcgcggcc ggcgagcggc gcgaccacca ggcgaaggct
     2221 caccgggtgc gccgtggggg tgagcgggac gtgccggcgc agcagcgcca tcagctgctt
     2281 ctgatcggac agcccgttgc gcttgacgag gtcccggacg ctgaggtgaa acacctcgcg
     2341 caaccgggcg tggtctcgcc agatctcggc gatccgatcg gcgccctcga cgagctggcc
     2401 ggccgccacc gccgaggcga gcaccgtccc ggtcagcgcg ccggagctcg ccgccacgat
     2461 ccgcaggatc tcgacgtcgg tgcgggccag cgcctggagc gcgccggcct cgaacgcacc
     2521 cttggccacc gcccccgcaa gcaccaccgc gatcttcttg gtcttgttgc ccatccgcgt
     2581 cctgacgaca gcgccgccgg accggacgcg tccggcgcag cgtaacacgc cggcgagatg
     2641 gactcatcga gaccgatcga gacgcctcag gcagggacgc ccgcgtccgc cgcgccgggc
     2701 tctccgaccg ggtcgggagg agcgccgccc tcgccggggc accccgaggg cccagcggcc
     2761 cggtgcggcc agccgagcgg g
//
LOCUS       DASVOR010002073         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_548419,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002073 DASVOR010000000
VERSION     DASVOR010002073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548419"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1291)
                     /gene="istA"
                     /locus_tag="VF516_38525"
     CDS             complement(<1..1291)
                     /gene="istA"
                     /locus_tag="VF516_38525"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEX8113710.1"
                     /translation="MTSTWRSREELVHQVVTLAVDRMSKRAIARAVGVSRNTVKAILA
                     AHAAQRSAEHSALPTPTAKAPRPKKTDAFRNRIGELLVRYPDITAQRVFEILGAEGFG
                     GGYTAVKKHMRTLRAPKKPAPSRVAPSYGPGEMAESDWSPYTIDFTAAGRLTVQAFSY
                     VLTYSRRKAFSVYLSSDLHALMDGHTHAFDRFGGCAQRCTYDSQKPVVLRWEGTQPIY
                     NPRFLAYAAHYEFQPRAVRGDPNAKPRVERGFWEFERSFLNGRSFRDLDDLHAQLVNW
                     LDGIVDPRRRHGQTCLERFAEEQPRLVPLPRHPYDTARVIYRVCSIDGFVDWEGNRYA
                     VPYDHVTDILPVRITQRELFVYAADLSCVARHELAPRGAGHKLDPAGLHPPPRGRSPV
                     DLDQLAVAFDGLGPGGVAFFRLLSAGPARVWGHQARCI"
     gene            complement(1288..>1520)
                     /locus_tag="VF516_38530"
     CDS             complement(1288..>1520)
                     /locus_tag="VF516_38530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113711.1"
                     /translation="PRPARTPRGEADGMVTDIATLVRVGARLQARLLGTPLGALGAPA
                     AEVVLDRLGALAPTLTALTRTVATVLGTEQAA"
BASE COUNT          213 a          521 c          567 g          219 t
ORIGIN      
        1 ggatgcagcg cgcctggtgg ccccacaccc gcgccgggcc cgcgctgagc aggcggaaga
       61 acgcgacgcc ccccgggccc aggccgtcga acgccacggc gagctggtcg aggtccactg
      121 ggctcctgcc gcgcggaggc ggatggagac cggcggggtc gagcttgtgc ccggcgccgc
      181 gcggcgcgag ctcgtggcgc gcgacgcacg acaggtccgc ggcgtagacg aacagctcgc
      241 gctgggtgat gcgcaccggc aggatgtcgg tcacatggtc gtagggcacg gcgtagcgat
      301 tgccctccca gtcgacgaag ccgtcgatgc tgcacacgcg atagatcacg cgcgcggtgt
      361 cgtacgggtg gcgcggcagc ggcacgaggc ggggctgctc ttcggcgaac cgctcgaggc
      421 aggtctggcc gtggcggcgc cgcgggtcga cgatcccgtc gagccagttg acgagctggg
      481 cgtgcaggtc gtcgaggtcg cgaaacgagc ggccgttgag gaatgaccgc tcgaattccc
      541 agaacccgcg ctcgacccgg ggcttggcat tcgggtcgcc gcgcacggcg cgcggctgaa
      601 attcataatg agcggcatat gccagaaatc gcggattgta gatcggctgt gtgccctccc
      661 agcgcagcac cacgggcttc tggctgtcgt aggtgcagcg ctgggcgcag ccgccgaagc
      721 ggtcgaacgc gtgcgtgtgc ccgtccatga gcgcgtgcag atcactggac aggtagaccg
      781 agaaggcctt gcgccggctg taggtcaaca cgtaggagaa cgcctgtacc gtcaggcgcc
      841 cggcggcggt gaagtcgatg gtgtacggcg accagtcgct ctcggccatc tcgccggggc
      901 cgtacgacgg cgcgacccgg ctcggcgccg gcttcttggg cgcgcgcagc gtgcgcatgt
      961 gcttcttgac cgcggtgtag ccgccaccga atccctccgc gccgaggatc tcgaacaccc
     1021 gctgggccgt gatgtccggg tagcggacga gtagctcgcc gatccggttc cggaacgcat
     1081 ccgtcttctt gggccgtggc gccttcgcgg tcggcgtggg cagcgccgag tgctccgcgc
     1141 tgcgctgcgc cgcatgcgcc gcgaggatag ccttgaccgt gttgcggctg acgccgaccg
     1201 cccgggcgat ggcgcgcttg gacatccggt cgaccgcgag cgtcacgacc tggtgcacga
     1261 gctcctcgcg cgaccgccag gtcgacgtca tgctgcctgc tccgtgccga gcaccgtcgc
     1321 gacggtccgt gtgagcgcgg tgagcgtcgg cgcgagcgca ccgagccggt cgagcacgac
     1381 ctcggccgcg ggtgccccga gcgcaccgag cggtgtcccg agcagccgag cctgcagccg
     1441 tgcacccacc cgcaccagcg tcgcgatgtc ggtcaccatg ccgtccgcct cgccgcgcgg
     1501 cgtgcgtgcc ggccggggtg
//
LOCUS       DASVOR010002074         5240 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_489740,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002074 DASVOR010000000
VERSION     DASVOR010002074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489740"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            253..2295
                     /gene="dnaG"
                     /locus_tag="VF516_38535"
     CDS             253..2295
                     /gene="dnaG"
                     /locus_tag="VF516_38535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEX8113712.1"
                     /translation="MIPDDVIAQIRDAADIVAVIGEHVQLRRAGTSFKGLCPFHGERT
                     PSFNVVPAKQFFHCFGCQKHGDVFTFVMELEGKSFVEAAEQLAGRFGIVIPRVEEPPE
                     LQRARVERLRMLEINKLAAAFYRELLLDPKRGEPGRAYLARRGVTAETAERFQLGYAP
                     AEWGALADHLKARRADLELAVKLGLIAHRPRTSGFYDRNRDRLVCPVLVPGGDIVGFS
                     SRLVGAPTPGPDGSEPPKYINSPESAVYKKGKLLFGLAQARDAMHTRKRAVLVEGNFD
                     VISLHQAGFTEVVAPLGTALTTEQIQVLKRLAERVVLLYDGDRAGYKATMHALQMCVE
                     ADVEVLVASRPGHARSGGAGILSGGVDPDSLIASGGAELLREAVDRAQGGIEFFAFEV
                     WGKARASADARARALEDAARLVGKIANPIKRDLIVGTLATAMDVDVGVVRAALARAGN
                     PHHAQGHRSSGPSMGPSMGPSSGPGGSGAAGPSGPGAGSARAPAPHPNAPSPQREAAT
                     SATGGPVAPPPTEELELITLLADHPALIATAEADKAFWLLTDARLRDMYSRAREGQSF
                     LELAPVQLPPPLAKHVLSGKYALAKDPASSLAAMTRNLEARKAGVGLANLKNSLVDAR
                     RRGDHDLARMLALRAVAERRGDRELADRLAEEVHAGSQAESRGPSDPETSNRKQVE"
     gene            2299..4653
                     /gene="rpoD"
                     /locus_tag="VF516_38540"
     CDS             2299..4653
                     /gene="rpoD"
                     /locus_tag="VF516_38540"
                     /inference="COORDINATES: protein motif:HMM:NF004208.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEX8113713.1"
                     /translation="MARTQVAKTKKPDPKAKKPPAAPAKEAPRKVEAKKEARAKPEAR
                     DGKDARPEGVEGKAGREGKDAKARPGAAESKDAAAGAKPGKPLTRPATKPPRPPRDED
                     DDGFDDEDDEDDEDFAPVAKPAKPGKPGKPGKAGRPAEIDLDDAGDDSVMPSFEDDEE
                     DEDFGNAPKPRSLVSEADAAAMEAETRKKPIRPGARAGGGDGNTARDKLIELGKQKGF
                     VTYDEVNDHMPEDVVSTDQIDGWLAALGEHGIEVVDGSGSRRPELVDQPPKGLDIEKE
                     KEEGEVDEDEEYAYSRTSDPVRMYLRKMGSVSLLTREGEVEIAKRIEDGERKMLQAVL
                     NSSVAVEELLEIGERLRRGKVRVKDVVKDIDEDEAEFNEQFYVDRVCKIIDKVRKLQR
                     DTEKLEEKLVEADTEGKKKKVKDLIKANRAQMFEELSDLRLNKPTVDRIVAQLKNIII
                     KLDKAELEVRECEARAGLSASEIRKTLREMRASPARARSVGKKTMLTIADFEEMEEQI
                     KTAMRKVAVIEEEARGSAPELRSTFRDLHEGERMADRAKSELVEANLRLVVSIAKKYT
                     NRGLQFLDLIQEGNIGLMKAVDKFEYKRGYKFSTYATWWIRQAITRAIADQARTIRIP
                     VHMIETINKLVRTTRYLVQELGREPIPEEIAERMELPLDKVRKVLKIAKEPISLETPI
                     GEEEDSHLGDFIEDKSIVSPSESVISVNLADQTRKVLATLTPREEKVLRMRFGIGEKS
                     DHTLEEVGQDFEVTRERIRQIEAKALRKLRHPSRSKRLKAFVEN"
     gene            4912..5085
                     /locus_tag="VF516_38545"
     CDS             4912..5085
                     /locus_tag="VF516_38545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113714.1"
                     /translation="MTEREAKSRAVKILAKSIYRDLEAQGFDEKQIVSLATELISEVT
                     AKMARGDKAQQVA"
BASE COUNT          965 a         1763 c         1836 g          676 t
ORIGIN      
        1 gcggacgtgt cgcccgaccg gcccgcgcgg cccggccgac cgtgacaccg cggccgtgac
       61 gcgcagcgct gtcgtgcacg gcttgcatcc cgcacgcgcc tgcggttttt gcgggtccgg
      121 gcgcgtgagc gctaccgctc ggtccgcgaa tcggtcagag ttattagatg tgcgcccacc
      181 ggggccggtc gcgccgggcc aaggacgttc cggacgccag gatcgaatga accgagaagc
      241 gagcatcggg cgatgattcc ggacgacgtg atcgcgcaga tccgcgacgc cgccgacatc
      301 gtcgcggtga tcggcgagca cgtccagctc cggcgcgcgg gcaccagctt caagggcctg
      361 tgcccgttcc acggcgagag gaccccgtcg ttcaacgtcg tcccggccaa gcagttcttc
      421 cactgcttcg gctgccagaa gcacggcgac gtgttcacgt tcgtgatgga gctcgagggc
      481 aagagcttcg tcgaggccgc cgagcagctc gccggccggt tcggcatcgt gatcccgcgc
      541 gtcgaggagc cgcccgagct gcagcgcgcg cgcgtcgagc ggctcaggat gctcgagatc
      601 aacaagctcg cggcggcgtt ctaccgcgag ctgttgctcg atcccaagcg cggcgagccc
      661 ggccgcgcct acctcgcgcg gcgcggcgtg accgccgaga ccgccgagcg gttccagctc
      721 ggctacgcgc cggcggaatg gggtgcgctg gcggaccatc tgaaggcccg gcgcgccgac
      781 ctcgagctcg ccgtcaagct cgggctgatc gcccaccggc cacggacgag cggattctat
      841 gaccgcaacc gcgaccggct ggtctgcccg gtgctcgtcc cgggcggcga tatcgtcggg
      901 ttctcgtcgc gcctggtcgg tgccccgacc cccggcccgg acggcagcga gccacccaag
      961 tacatcaaca gccccgagag cgcggtctac aagaagggca agctgctgtt cggcctcgcc
     1021 caggcgcgcg acgcgatgca cacccgcaag cgcgccgtgc tggtcgaggg caacttcgac
     1081 gtgatctcgc tgcaccaggc cgggttcacc gaggtggtcg cgccgctcgg caccgcgctg
     1141 acgaccgagc agatccaggt gctcaagcgg ctcgccgagc gggtcgtgct gctctacgac
     1201 ggcgaccgcg ccggctacaa ggcgacgatg cacgccctgc agatgtgcgt ggaggccgat
     1261 gtcgaggtcc tggtcgcgtc gcggcccggc cacgcccggt ccggcggcgc gggcatcctg
     1321 tccggcgggg tcgatccgga cagcctgatc gcgtccggcg gcgcggagct gctccgcgag
     1381 gcggtcgacc gcgcccaggg cggcatcgag ttcttcgcgt tcgaggtctg gggaaaggcg
     1441 cgcgccagcg ccgacgcccg ggcccgggcc ctcgaagacg ccgcgcggct ggtcgggaag
     1501 attgcaaacc cgatcaaacg tgatctaatc gtcggcacct tggcgaccgc aatggacgtg
     1561 gacgtcgggg tggtgagagc tgcactggcc cgggccggga acccgcacca cgcgcagggt
     1621 catcgctcct ctggcccctc gatgggtcca tcgatgggtc catcgagcgg tccgggcggc
     1681 tccggcgctg cgggtccttc gggtccgggc gccggctccg cgcgcgcccc tgcgcctcat
     1741 cccaacgccc cgtcaccaca gcgagaagcc gcgacgtctg cgaccggcgg gcccgtcgca
     1801 ccgccgccca cggaggagct ggagctgatc acgctcctgg ccgatcatcc agctctgatc
     1861 gcaaccgccg aagcagacaa ggctttttgg ctcttgacgg acgcacgcct tcgcgacatg
     1921 tattcccgcg cccgggaggg acaatccttc ctggagcttg ctcctgtgca attgcccccg
     1981 cctctggcca agcacgtgct gtccgggaaa tacgctctcg ccaaggaccc ggcttcctcg
     2041 ctggccgcga tgacgcgcaa cctggaagct cgcaaggcgg gggtgggcct ggcgaacctg
     2101 aagaacagcc tcgtcgacgc gcggcgtcgc ggggaccacg acctcgcgcg catgctggcg
     2161 ctgcgcgcgg tggcagaacg cagaggcgac cgcgagctgg ccgatcggct cgcggaggaa
     2221 gtccacgctg gatcgcaggc cgagagccgc ggtccgtccg accccgagac gtcgaaccga
     2281 aagcaggtcg agtagattat ggcgcgtacc caggtcgcaa aaaccaagaa gccggatccg
     2341 aaggcgaaga agccccccgc ggcccctgcg aaagaagcgc ccaggaaggt cgaggccaag
     2401 aaggaggccc gggcgaagcc ggaagcgcga gacggcaagg acgcccggcc ggagggggtc
     2461 gaagggaaag ccgggaggga gggcaaggac gccaaggccc ggcccggcgc cgcggagtcc
     2521 aaggatgcgg cggccggcgc caaacccggc aagccgctga cccggccggc gaccaagccg
     2581 ccccggccgc cgcgcgacga ggacgatgac gggttcgacg acgaggatga cgaggacgac
     2641 gaggacttcg cgccggtcgc caagccggcc aagcccggca agccgggcaa gcccggcaag
     2701 gccggccgcc ccgcggagat cgatctcgac gacgccggcg acgacagcgt gatgccgagc
     2761 ttcgaggacg acgaggagga cgaggatttc ggcaacgcgc ccaagccgcg cagcctggtc
     2821 agcgaggccg acgccgccgc gatggaggcc gagacccgca agaagccgat ccggcccggc
     2881 gcgcgcgcgg gcggcggcga cggcaacacc gcgcgcgaca agctgatcga gctcggcaag
     2941 cagaagggct tcgtgaccta cgacgaggtc aacgaccaca tgcccgagga cgtggtgtcg
     3001 accgaccaga tcgacggctg gctcgccgcg ctcggcgagc acggcatcga ggtcgtcgac
     3061 ggctcgggct cgcgccgccc cgagctggtc gatcagccgc ccaagggcct cgacatcgag
     3121 aaggagaagg aggagggcga ggtcgacgag gacgaggagt acgcctactc gcggaccagc
     3181 gatccggtcc gcatgtacct gcgcaagatg ggctcggtgt cgctgctgac ccgcgagggc
     3241 gaggtcgaga tcgccaagcg gatcgaggac ggcgagcgca agatgctgca ggccgtgctc
     3301 aacagctcgg tcgccgtcga ggagctgctc gagatcggcg agcggttgcg ccgcggcaag
     3361 gtccgggtca aggacgtggt caaggacatc gacgaggacg aggccgagtt caacgagcag
     3421 ttctacgtcg accgggtgtg caagatcatc gacaaggtcc gcaagctcca gcgcgacacc
     3481 gagaagctgg aggagaagct ggtcgaggcc gacaccgagg gcaagaagaa gaaggtcaag
     3541 gacctgatca aggccaaccg cgcccagatg ttcgaggagc tgtccgacct ccggctcaac
     3601 aagccgacgg tcgatcgcat cgtcgcccag ctcaagaaca tcatcatcaa gctcgacaag
     3661 gccgagctcg aggtccgcga gtgcgaggcc cgggccggtc tgtcggccag cgagatccgc
     3721 aagacgctgc gcgagatgcg cgcgtcgccg gcgcgcgccc ggtcggtcgg caagaagacc
     3781 atgctgacga tcgccgactt cgaggagatg gaggagcaga tcaagacggc gatgcgcaag
     3841 gtcgccgtga tcgaggagga ggcccgcggc tcggcccccg agctgcgctc gacgttccgc
     3901 gatctccacg agggcgagcg catggccgat cgcgccaagt ccgagctggt cgaggccaac
     3961 ctccggctcg tggtgtcgat cgccaagaag tacaccaacc gcggactgca gttcctggac
     4021 ctgatccagg agggcaacat cggcctgatg aaggcggtcg acaagttcga gtacaagcgc
     4081 ggctacaagt tctcgaccta cgcgacgtgg tggatccgcc aggcgatcac gcgcgcgatc
     4141 gccgaccagg cccggacgat ccgcatcccg gtccacatga tcgagacgat caacaagctg
     4201 gtgcgcacca cgcgctacct ggtccaggag ctcggccgcg agccgatccc cgaggagatc
     4261 gccgagcgca tggagctgcc gctcgacaag gtccgcaagg tcctcaagat cgccaaggag
     4321 ccgatctcgc tggagacccc gatcggcgag gaggaagact ctcatctcgg cgacttcatc
     4381 gaggacaagt cgatcgtctc gccgagcgag agcgtgatct cggtgaacct cgccgatcag
     4441 acccgcaagg tgctcgccac gctcaccccg cgcgaggaga aggtcctgcg catgcggttc
     4501 ggcatcggcg agaagtccga ccacacgctc gaggaggtcg gccaggactt cgaggtcacc
     4561 cgcgagcgca tccgccagat cgaagcgaag gcgctccgca agctgcgcca cccgtcgcgg
     4621 tccaagcggc tcaaggcctt cgtcgagaac tgaggcgaag aaccgcgcga cggcccggat
     4681 cgatcgacgg aaacccgcgc gaaaccaggt gctcggcaca cacggccgaa tggtccgacc
     4741 gtgtgcgtag cgcgttatgc actgattcct ccgctatgca ggagagtgct tcgcggtgag
     4801 aagatcttac aggatgcccc tctggagcta tcacgtgatt ctcacggctt gccacgcggc
     4861 cggactgggc atgatctggc acagcggctg cttcgttgtg cggcaccgac catgaccgag
     4921 cgcgaagcca agtcccgagc ggtgaagatc ctcgccaagt cgatctaccg cgacctggag
     4981 gcacagggct ttgacgagaa gcagatcgta tcgctggcca ccgagttgat cagcgaggtc
     5041 acggccaaga tggcgcgcgg cgacaaggcc cagcaggtcg cctagcgaac cgcgaaaaaa
     5101 atccgggcag gaacgcacgg cctgcgcgtt cctgatggtc cggacatggg cgtggaacgc
     5161 gctgaaacgc gtgacccagg cctcgcgatg cggcgcctga tctcgacccg atgatgctgg
     5221 cggtacatgg cgatccgatc
//
LOCUS       DASVOR010002075         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_314661,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002075 DASVOR010000000
VERSION     DASVOR010002075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314661"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="VF516_38550"
     CDS             <1..422
                     /locus_tag="VF516_38550"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEX8113715.1"
                     /translation="TELSPAAAALARRNAARNAVADRVDVREGDLFEPVRGERFDLIA
                     ANPPYIASAVIPTLSPEVRREPVIALDGGLDGLAFYDRICAGAREHLAPGGAIVLEHG
                     FDQADAVRARLIAAGFTGVTLVHDLGKNPRVTRGNAP"
     gene            complement(426..821)
                     /locus_tag="VF516_38555"
     CDS             complement(426..821)
                     /locus_tag="VF516_38555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8113716.1"
                     /translation="MTEPPRLFRVTLEVANLDQSTQLYSELLGIPGKRHPGARHYYDC
                     GGVILAVLDVSRGALPPTPGPKSLYFAVSDLDAVHARAVRLGILAPYQVHGQPAGDVI
                     TRPWGERSFYVVDPWGNDLCFCEDGTLYT"
     gene            complement(818..1213)
                     /locus_tag="VF516_38560"
     CDS             complement(818..1213)
                     /locus_tag="VF516_38560"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8113717.1"
                     /translation="MSRAAEPPEIPAAPTLAEIEAVFAALSHEVRRHIVQLLSHLGGE
                     LPSGYLAKRFSHSWPTTTRHLHVLEAAGLVTVRREGRSSVYRLERDRLRRIVGGWLAA
                     LDPPTPQQTWRSSGPRSVADRPAPRGRPR"
BASE COUNT          204 a          548 c          599 g          204 t
ORIGIN      
        1 cgaccgagct gtcgcccgcc gccgcggcgc tggcccgccg caacgccgcg cgcaacgcgg
       61 tcgccgatcg cgtcgacgtg cgcgagggcg atctgttcga gccggtgcgc ggcgagcggt
      121 tcgatctgat cgccgccaac ccgccgtaca tcgcgtcggc ggtgatcccg acgctgtcac
      181 ccgaggtccg ccgcgagccg gtgatcgcgc tcgacggcgg cctcgatggc ctggcgttct
      241 acgaccggat ctgcgccggg gcccgcgagc acctcgcacc cggcggcgcg atcgtcctcg
      301 agcacggctt cgaccaggcc gacgcggtcc gcgcgcggct gatcgcggcc gggttcaccg
      361 gcgtgaccct ggtccacgac ctcggcaaga acccgcgggt cacgcggggc aacgcgccgt
      421 gaccgtcacg tgtacagcgt gccgtcctcg cagaagcaca ggtcgttgcc ccacggatcg
      481 acgacgtaga acgagcgctc gccccacggc cgggtgatca cgtcgccggc gggctggccg
      541 tgcacctggt agggcgcgag gatgccgagc cgcacggcgc gcgcgtggac cgcgtcgaga
      601 tcggacaccg cgaagtacag cgacttgggg ccgggcgtcg gcggcagcgc gccgcgcgac
      661 acgtcgagca cggcgaggat cacgccgccg cagtcgtagt agtggcgcgc gcccgggtgg
      721 cgcttgccgg ggatgccgag cagctcgctg tacagctggg tggactgatc gaggttggcc
      781 acctcgagcg tgacgcggaa caggcggggg ggttcagtca tcgcggtctt cctctggggg
      841 cggggcgatc ggcgaccgag cgcggccccg aggagcgcca ggtctgttgc ggggtgggcg
      901 gatcgagcgc ggcgagccag ccgccgacga tgcggcggag ccggtcgcgc tcgaggcggt
      961 agacgctgct gcgaccctcg cgccgcacgg tgaccaggcc ggccgcctcg aggacgtgca
     1021 ggtggcgggt cgtggtcggc cagctgtgcg agaaccgctt ggcgaggtag cccgacggca
     1081 gctcgccgcc gaggtgcgag agcagctgga cgatgtggcg gcggacctcg tgcgacagcg
     1141 cggcgaacac cgcctcgatc tcggcgaggg tgggcgccgc cgggatctcc ggaggctcgg
     1201 cggcgcgcga catgggccca ttgttacctc accggctaat aattagcaag aaggctaagt
     1261 cacggcgcac gcggatccgg caaggtcggg caggcgtgtc cgcgatgtgc gcagctccgg
     1321 gcagctgcgg ggcatggcgg ccgtcgtgaa ccggctgctt cgatccccgt cacgcgatcc
     1381 accgcgggcg cggcgcgatc cgcccacgcg gcgtccggct tgtgtacgat gccgtcgtgc
     1441 gccgcatcgc gcttgtctgc gtcctgctcg caggcagcgc gcgcgctggc gcgcaggtcg
     1501 ccgctccgcc ggccccgggg gcatccgatc cggccgctcc cggatcgccc gcatc
//
LOCUS       DASVOR010002076         1580 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_284020,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002076 DASVOR010000000
VERSION     DASVOR010002076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284020"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="VF516_38565"
     CDS             <1..330
                     /locus_tag="VF516_38565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113718.1"
                     /translation="GDPGRLLTGASCVVAELHCLRCYWTQCEKLFTTPEAAEQYMHQP
                     PDEPDAASDIWDAAELTADRSEPLRHWRSVLGEVPLHHLRLSTSLETIDVICEQIEVR
                     IDPRRRA"
     gene            complement(347..>1580)
                     /locus_tag="VF516_38570"
     CDS             complement(347..>1580)
                     /locus_tag="VF516_38570"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8113719.1"
                     /translation="EKRVPDLAGMLQHADLRGWLPCVDGLDTIPSDDAATRGQVRELL
                     RDHVGPLAVRLGRHVAPPLVPGYVLIELPTSTIAERAAQWTEILRGAGIAVRDVDELA
                     ARFTVGPGTIKGVVASVARGAPPDPDQAIETALRQYLESKLGTVATRVTRLATWSQIV
                     LPADIQDSIVELVARIRHRRTVYDTWGFDQVMSTSRGLTALFQGGPGTGKTLVASAIA
                     NELGLDLYRIDLSRVMSKWIGETEQNLAKVFDAAEEGQALILFDEADSLFGKRTEVRT
                     SVDRYANLETNFLLQRLDSFEGVAVLTTNFGTAIDAAFKRRLSCRLTFPFPDDEARER
                     LWRVHLPEQLPLAGKLDLADLARRYKMSGGYIRNAALRAAFLAAEEQVPLSQDHLERA
                     VRAEFREGGKLAESGFLE"
BASE COUNT          221 a          524 c          616 g          219 t
ORIGIN      
        1 ggagatccgg ggcggctgct gaccggcgcg agctgtgtcg tcgccgagct gcactgcctg
       61 cgctgttact ggacccagtg cgagaagcta ttcacgacgc cggaggcagc ggagcagtac
      121 atgcaccagc cgcccgacga accggacgct gcgtccgata tctgggacgc cgcggagctg
      181 accgctgacc gctccgagcc gctgcgacac tggcggtcgg tgctcggcga ggtgccgctg
      241 caccacctcc ggctgtcgac cagcctggag accatcgacg tgatctgtga gcagatcgag
      301 gtccggatcg atccgcgccg ccgcgcgtga cgccggggcc cgggcgctac tcgaggaagc
      361 cggactcggc gagcttgccg ccctcgcgga actcggcgcg cacggcgcgc tcgaggtgat
      421 cctgcgacag cgggacctgc tcctcggcgg cgaggaacgc ggcgcgcagc gcggcgttgc
      481 ggatgtagcc gcccgacatc ttgtagcggc gcgcgaggtc ggcgaggtcg agcttgccgg
      541 cgagcgggag ctgctcgggc aggtggacgc gccacagccg ctcgcgggcc tcgtcgtcgg
      601 ggaacgggaa ggtcagccgg cacgacagcc ggcgcttgaa cgcggcgtcg atcgcggtgc
      661 cgaagttggt ggtgagcacg gcgacgccct cgaagctgtc gagccgctgc agcaggaagt
      721 tggtctcgag gttggcgtag cgatcgaccg aggtccggac ctcggtgcgc ttgccgaaca
      781 gcgagtcggc ctcgtcgaac aggatcagcg cctggccctc ctcggcggcg tcgaacacct
      841 tggcgaggtt ctgctcggtc tcgccgatcc acttggacat cacgcgcgac aggtcgatgc
      901 ggtagaggtc gaggccgagc tcgttggcga tcgcgctggc gaccagggtc ttgccggtgc
      961 ccgggccgcc ctggaacagc gcggtcaggc cgcgcgaggt cgacatcacc tgatcgaagc
     1021 cccaggtgtc gtagaccgtg cggcggtggc ggatccgggc gacgagctcg acgatgctgt
     1081 cctggatgtc ggccggcagc acgatctgcg accaggtcgc gagccgggtg acccgggtcg
     1141 cgaccgtgcc gagcttgctc tcgaggtact ggcggagcgc ggtctcgatc gcctgatcgg
     1201 ggtcgggcgg cgcgccgcgc gcgaccgagg cgacgacgcc cttgatcgtg ccggggccga
     1261 cggtgaaccg cgcggccagc tcgtcgacgt cgcgcaccgc gatgccggcg ccgcgcagga
     1321 tctcggtcca ctgcgcggcg cgctcggcga tcgtcgaggt cggcagctcg atcaggacgt
     1381 agccggggac cagcggcggc gcgacgtggc ggcccagccg gacggcgagc gggccgacgt
     1441 gatcgcggag cagctcgcgg acctggccgc gggtcgcggc gtcgtcggac gggatcgtgt
     1501 cgaggccgtc gacgcacggc agccagccgc gcaggtcggc gtgctggagc atgccggcga
     1561 ggtcggggac gcgcttctcg
//
LOCUS       DASVOR010002077         1811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_430464,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002077 DASVOR010000000
VERSION     DASVOR010002077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1811
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430464"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..>1811)
                     /locus_tag="VF516_38575"
     CDS             complement(177..>1811)
                     /locus_tag="VF516_38575"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8113720.1"
                     /translation="LGAVLDGTLQAAWRTVELAAATWADAPGDRRETQLLLRRLKADV
                     PIIRALSILDLDGQVRSGDPVPAKLDIGSHSYGGYVGDIEDSGGRPVVYLVVQARSRT
                     GELIGVFVAGLDLEFVGKLISETQLQPGVRVIVVDGTGAVVASSEDRAAAAAPPHRRS
                     LAGVDPAVDRALGSTVEGTLSANGWVSAYRNLSSYQSHRGVRWAILHQQPERDAYALA
                     RETTRDTLIIGGAALAIALALGWFFATRLTRPLAALARHADAIAAGSIDDPPPVRGPG
                     EIGLLGERIDEMARRIAERAALQAALARGDRLASVGVMSAQVAHEINNPLTTVLGYAM
                     LLQERKPPGHPDREALQLIASEAERMKSIVGGLLEYARAHRKGARDLAASASPTPGTC
                     DPAAVLRHVGALLEPQLRRTRTSLAIDVAATAPVAIEAHALQQVLVNLGQNAAQAMAE
                     AQLEGGTLSITARPAPGGIATLITVTDEGPGVPPPDRARVFDPFFTTKAAGVGTGLGL
                     AVCKHLVTTAGGSIEVGDGPGGRGAEFRVVIPNASA"
BASE COUNT          227 a          575 c          789 g          220 t
ORIGIN      
        1 cggacccgct gcgatggctg cggcgcgcag gtcacgggcc gagccaccat ccacaggcag
       61 catgagatct cccgatgctc cacctcgcag ccggtaggtc agctgagcgg agcacgtggt
      121 ggagcagacg agtcgacggc cgcggccacc cttcggcgag gccgcgcaga cgctgatcac
      181 gccgatgcgt tggggatcac gacgcggaac tcggcgccgc ggccgcccgg gccgtcgccg
      241 acctcgatcg agccgccggc ggtggtgacc aggtgcttgc agacggccag gcccaggccg
      301 gtgccgacgc cggcggcctt ggtggtgaag aacggatcga acacgcgggc gcgatcgggc
      361 ggcgggacgc cggggccctc gtcggtgacg gtgatcaggg tggcgatgcc gcccggggcg
      421 gggcgggcgg tgatcgagag cgtgccgccc tcgagctggg cctcggccat ggcctgggcg
      481 gcgttctggc cgaggttgac caggacctgc tggagggcgt gggcttcgat ggcgaccggc
      541 gcggtggcgg cgacgtcgat cgcgagggag gtccgggtcc ggcgcagctg gggctcgagc
      601 agcgcgccga cgtggcgcag caccgcggcg ggatcgcagg tgccgggggt ggggctggcg
      661 gacgcggcga ggtcgcgggc gcccttgcga tgggcgcggg cgtactcgag gaggccgccg
      721 acgatcgact tcatgcgctc ggcctcgctg gcgatcagct ggagggcctc gcgatcgggg
      781 tggccgggcg gcttgcgctc ctggaggagc atggcgtagc cgagcacggt ggtgagcggg
      841 ttgttgatct cgtgggcgac ctgggccgac atgacgccga ccgaggcgag ccggtcgccg
      901 cgcgcgaggg cggcctggag ggcggcgcgc tcggcgatcc ggcgcgccat ctcgtcgatc
      961 cgctcgccga gcaggccgat ctcgcccggg ccgcggacgg ggggcgggtc gtcgatcgag
     1021 ccggcggcga tggcgtcggc gtggcgggcg agggcggcga gcgggcgggt gaggcgggtg
     1081 gcgaagaacc agccgagggc gagggcgatg gcgagcgcgg cgccgccgat gatgagggtg
     1141 tcgcgcgtgg tctcgcgggc gagggcgtag gcgtcgcgct cgggctgctg gtggaggatc
     1201 gcccagcgca cgccgcggtg cgactggtag ctggacaggt tgcggtaagc gctgacccag
     1261 ccgttggcgg acagggtgcc ctcgacggtc gagcccaggg cgcgatcgac cgcggggtcg
     1321 acgcctgcga gcgagcggcg gtggggcggc gcggcggcgg cggcgcggtc ctcgctggac
     1381 gcgaccaccg cgccggtgcc gtcgacgacg atgacgcgga cgccgggctg gagctgggtc
     1441 tcggagatca gcttgccgac gaactcgagg tcgaggccgg cgacgaacac gccgatcagc
     1501 tcgccggtgc ggctgcgcgc ctggacgacc aggtagacga ccgggcggcc gcccgagtcc
     1561 tcgatgtcgc cgacgtagcc gccgtagctg tgcgagccga tgtcgagctt ggccgggacc
     1621 gggtcgccgg atcggacctg gccgtcgagg tcgaggatcg acagcgcgcg gatgatcggg
     1681 acgtcggcct tgagccggcg gagcaggagc tgggtctcgc gccgatcgcc gggggcgtcg
     1741 gcccaggtcg cggccgcgag ctcgacggtg cgccacgcgg cctggagggt gccgtcgagc
     1801 acggcgccga g
//
LOCUS       DASVOR010002078         8357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_460162,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002078 DASVOR010000000
VERSION     DASVOR010002078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460162"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            460..600
                     /locus_tag="VF516_38580"
     CDS             460..600
                     /locus_tag="VF516_38580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113721.1"
                     /translation="MVVNGRPNEVACPHCNHRPLTIEEIDQSTRISCSKCGKFIQGRF
                     AH"
     gene            605..1066
                     /locus_tag="VF516_38585"
     CDS             605..1066
                     /locus_tag="VF516_38585"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX8113722.1"
                     /translation="MPNDKDFLGKGLRFPVSINLNGGVSTSQYEENVRQSLFVILGTA
                     PGERINRPRFGCRIHDLMFAPNNQVTAARAEVFCEEAILKFEPRVEKVVCRAGPNPDE
                     PNRLDVRIEYVIAGKNDQKRNLVFPFYLKPEDESEPAPDGISVSASVVNNA"
     gene            1082..4120
                     /locus_tag="VF516_38590"
     CDS             1082..4120
                     /locus_tag="VF516_38590"
                     /inference="COORDINATES: protein motif:HMM:TIGR02243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative baseplate assembly protein"
                     /protein_id="HEX8113723.1"
                     /translation="MIPPPKLDDRTFNDIVEEAISMIPRYAPEWTNHNPSDPGITLIE
                     LAAWMTDLLIYRLNQVPDKNYVAFLNLLGIKLRAPRAARALVRFSLVEGTVKQRVPRG
                     TQISTPQATEEHTVTFETSRDVVVCAARPDRCFSYFSNSYSENSRFIDPAPGTAAAPF
                     EVFSGAQRIDRFIYLSDPRLANAGEASLLRVFLGAPEHGGRDLARFLEWEYWEGTRWK
                     DLEPAQIEVDRGEIAFLGPLRFEPTTVHHIEGLWMRGRLAEIPESEKDTEIDTIRVRV
                     EVVGEGLLPTQAYANLDNNAFLTLDLGKNLYPFGKEPKVDCILYLACDELLGTADAYI
                     SIEMLLADSSVIPRPNPSDQLVLAFEYWDGKRWRHLGRSAPRGALPGSGDELGFHDDT
                     KALSQSGTVSFRRPKDMEALELNGQTKRWVRIRIEKGDYGEQGTYTLENEKWVFKDDR
                     QLRPPALRAITFRYREDYRDVKHALAFNDFQFTDVTEVARTEYTIFQPFQAKAEESPA
                     LYLGFVAKPPSDMLGLHFALEEELGLGSLPTDEAEVATTELDKYETMRRLSWESGQRV
                     VWEYWDGRDWRTLSVNDETQGFTSSGFVFFNASDDWVISSKFTEERFWLRARLEQGGY
                     VKPPRVRMIVTNAIDAFNQETIADEILGSSDGSPLQQFKFLRSPLLEDEVIEVRERQP
                     PPADEVADLAADPVRKVQPDNPQNNEVWVRWKRVDSFFASGPRSRHYTLDYVSGTVTF
                     GDGRRGAVPPEAKDNIHTRPYRIGGGALGNVNAHTLTSLGRALAYIESVTNPLPATGG
                     ADRETIDEAKDRAPYTIKSRDRAVTSEDYETLALRASTTLARARCVPDRSNRGHVTLA
                     LVPKGELRGEEFSRRLVPSNEVLRYVKRYLDERKLVGTVLNVVRPRYKDLSLRVVLIR
                     RTVGTSDRLRKDIEHKLRKFLHSLTGGRDGKGWEFGRPVLKAELIHLVEEVPGVEGVD
                     ALEIRDELRNVGVEHLRLEDDELPFLVHVNVAEKVRDDIK"
     gene            4132..5022
                     /locus_tag="VF516_38595"
     CDS             4132..5022
                     /locus_tag="VF516_38595"
                     /inference="COORDINATES: protein motif:HMM:NF021220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8113724.1"
                     /translation="MATELLNRRVIRVPNVLGRSVTKARILLEDAGFKKIVTLYRESY
                     EDRDTVLDQKPPRTQMVYEDTEVTIWVARRGYLENLPAIYRRSDAVGRNLVRELCFVF
                     EHMFDSVEVNLTDGWRFYDPHVAPLDFLNWLSRWTAFSLDLDWPEPQKRALIKRAVDL
                     YRIRGTKRGLALFLRLFTGHEPDIEENTWPFKGFRVEGEDAESGARVGLDSVIMPPVD
                     LAHCFIVTMPMKYEDVTTDMVIRIHQIIQAEKPAHTHYYLRFAEEKGEVELREFFAIG
                     LRSGIGIGAEVVQSIDEEKS"
     gene            5019..6560
                     /locus_tag="VF516_38600"
     CDS             5019..6560
                     /locus_tag="VF516_38600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113725.1"
                     /translation="MSDAPQQGFKRINFFKGFLTTEKDWNDAERYHIDKRRLHNRMLH
                     SPGIVYGFAGDLRVNARARGDLSVEVQPGYAIDGMGNDLMIFDATIRNINLEEFRLPQ
                     TVYVVLRYYEELTDFIAYKENLEYKGHRRVLESCRVELSQTEPDIAREVELARIYLEK
                     GVQRVRDARDPANPAPNEIDMRYTPRGGLSGSSLPPHLRLRLELLLLAIRRGGLEYTR
                     RGVIAAHDLVQACNTALMLLYANLLDLRNIFDIFKLIIDCEGELALDVDVHHPTIAQK
                     KEFSEYRRQVEIGRGLLAEGKNNMEAFQNLLAFQNKASEIAQSAVAGEKPQIEAPPEK
                     KDAAPKVADIKDWEGVKTMPQPKPSMDLEGLTWVLVDEIEILNKESEDKHLFQIKEAK
                     DSYRSRQKLKYPDGTTVEDTGRAHVDGYSEFKLSNITPGRPVVVLRRMDYVYGDYELE
                     LSVNGKKVGIVSCVGTDRVHRWRNWPALIGVENVTTENLIVRQQSVTAGRDINMFHIW
                     VYQPK"
     gene            complement(6623..6943)
                     /locus_tag="VF516_38605"
     CDS             complement(6623..6943)
                     /locus_tag="VF516_38605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HEX8113726.1"
                     /translation="MPGQCRLGDKSNVPADAHGCPACPHPAVGPAIIGSTDVNVNKLP
                     AIRVDDTGIHMACCGPNTWTAKVGSGTVFINGKAAHRMGDQDQHCGGSGQMIEGSTNV
                     IVGG"
     gene            complement(6966..7778)
                     /locus_tag="VF516_38610"
     CDS             complement(6966..7778)
                     /locus_tag="VF516_38610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113727.1"
                     /translation="MQQLARLKFTSFSLKVPQNWKDPQGSAADHYGRAFKPDEKASAP
                     GMPPLFQPASLNKYHTDTQKMHIGKIGAFIDGTCSAICSAWSQWQTAATMVGVVINAV
                     TAMGGQIVGPPWTPLILASAPKSTPMEMKYSNVIANVIGTAWLSFTATVKVTGMPWYP
                     AFAAVPSPVAPPAPNVPCPFAALTQVPMSISCNVLKMQMVGQLGDPNAPFHQELFESI
                     ADAFEKSYNLWKVSTMVTNVLGTGPIPTFAPPYVPVGPVVGGVGTMTPGGFT"
     gene            complement(7860..>8357)
                     /locus_tag="VF516_38615"
     CDS             complement(7860..>8357)
                     /locus_tag="VF516_38615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113728.1"
                     /translation="GERVEYARRVVGRRYAAAQVAAGDRAVPADRRLLVCGDALDPPL
                     VPGVFGRVVALNLLDSVASPRQLLAVLDGLCQPGGEIIVSSPYAWQSSVMADHERLGW
                     GAGWCAMMDATDPAAELTALLRAGTGLAARYEIEDEAELPWTLRRDARTLASYSIHYV
                     RARKM"
BASE COUNT         1420 a         2892 c         2834 g         1211 t
ORIGIN      
        1 ggcaccggct cgacggccgg ccgcgatccg ccgccccgcg cccggcatcc gatggggagg
       61 cgcccgaagg tttcggagac gagtgcggcg gggccggcca tggcccggtc gtgacgccgc
      121 gccggcggaa cgggatccgt cgccgcgcgc ttgcacgccc gccggaggtg agttcgccta
      181 ccgaaaggcc cggcgcatga cgggatgggg cacaagggag gatgccggcg tacggcaacc
      241 gctgccaggc cgccgccgtg gctcgacggc cgcgacacgc gcgcgtcgcg cgcgcgtcga
      301 agcagcgtcg gatggggtgg tcgccgggcg agcgaattgg agcccgacgc gttgaccgcg
      361 cccgccggtg ttcttacgat tcaacgttcg aaggggtcga aacgaaagga ctctcgcgtg
      421 gacgaggaag cccttattgt gtggcaagac gtactggaca tggtggtcaa tggccggccg
      481 aacgaggtcg catgtccgca ctgcaaccac cgtccgctga cgatcgagga gatcgaccag
      541 tcgacgcgga tctcgtgcag caagtgcgga aagttcatcc agggccggtt cgcccactaa
      601 ccgcatgccg aacgacaagg acttcctcgg caaggggctt cggttccccg tgtcgatcaa
      661 cctcaacggc ggcgtctcca cctctcagta cgaggagaac gtgcgccagt cgctgttcgt
      721 catcctgggc accgcgccgg gcgagcggat caaccgcccg cggttcggct gccggatcca
      781 cgacctgatg ttcgcgccga acaaccaggt caccgcggcg cgcgccgagg tgttctgcga
      841 ggaggcgatc ctcaagttcg agccgcgggt cgagaaggtc gtgtgccggg ccgggcccaa
      901 cccggacgag cccaaccggc tcgacgtccg catcgagtac gtgatcgcgg gcaagaacga
      961 ccagaagcgc aacctcgtgt tcccgttcta cctgaagccc gaagacgagt cggagccggc
     1021 cccggacggg atcagcgtca gtgcctcggt cgtcaacaac gcctagcagc gagccaatcc
     1081 catgattcca ccgccgaagc tggacgaccg gaccttcaac gacatcgtcg aggaagccat
     1141 ctcgatgatc ccgcgctacg cgccggagtg gacgaaccac aatccgtccg atccggggat
     1201 cacgctgatc gagctcgcgg cgtggatgac cgacctgctg atctaccggc tcaaccaggt
     1261 cccggacaag aactacgtcg cgttcctcaa cctgctcggc atcaagctgc gggcgccgcg
     1321 ggcggcgcgc gcgctggtcc ggttcagcct ggtcgagggc acggtcaagc agcgcgtgcc
     1381 gcgcggcacc cagatctcga cgccgcaggc gaccgaggag cacaccgtca cgttcgagac
     1441 ctcgcgcgac gtcgtggtgt gcgcggcgcg ccccgatcgc tgcttctcgt acttcagcaa
     1501 cagctactcg gagaacagcc ggttcatcga tccggcgccc ggcaccgccg ccgcgccgtt
     1561 cgaggtgttc tccggcgcgc agcgcatcga ccgcttcatc tacctgtcgg acccgcggct
     1621 ggccaacgcc ggcgaggcct cgctgctgcg cgtgttcctc ggcgcgcccg agcacggcgg
     1681 ccgcgacctc gcgcggttcc tcgagtggga gtactgggag ggcacgcggt ggaaggatct
     1741 cgagccggcg cagatcgagg tcgaccgcgg cgagatcgcg ttcctcgggc cgctgcgatt
     1801 cgagccgacc acggtgcacc acatcgaggg cctgtggatg cgcggccggc tcgccgagat
     1861 cccggagtcc gagaaggaca ccgagatcga cacgatccgc gtgcgggtcg aggtcgtcgg
     1921 cgaggggctg ctgccgaccc aggcctacgc caacctcgac aacaacgcgt tcctgaccct
     1981 cgatctgggc aagaacctgt acccgttcgg caaggagccg aaggtcgact gcatcctgta
     2041 cctggcgtgc gacgagctgc tcggcaccgc ggacgcctac atctcgatcg agatgctgct
     2101 cgccgactcc agcgtgatcc cgcggcccaa tccgtccgat cagctggtgc tggcgttcga
     2161 gtactgggac ggcaagcgct ggcgccacct cggccgctcg gcgccgcgcg gcgcgctgcc
     2221 gggcagcggc gacgagctcg ggttccacga cgacaccaag gcgctgtcgc agtccggcac
     2281 ggtgagcttc cgccggccca aggacatgga ggcgctggag ctcaacggcc agaccaagcg
     2341 ctgggtccgg atccggatcg agaagggcga ctacggcgag cagggcacct acacgctcga
     2401 gaacgagaag tgggtgttca aggacgaccg ccagctccgg ccgcccgcgc tgcgcgcgat
     2461 cacgttccgc tatcgcgagg attaccgcga cgtcaagcac gcgctcgcgt tcaacgactt
     2521 ccagttcacc gacgtcaccg aggtcgcgcg caccgagtac acgatcttcc agccgttcca
     2581 ggccaaggcc gaggagtcgc cggcgctgta cctcgggttc gtcgccaagc cgccgtccga
     2641 catgctcggg ctgcacttcg cgctcgagga ggagctcggg ctcggctcgc tgccgaccga
     2701 cgaggccgag gtcgcgacca ccgagctcga caagtacgag acgatgcgcc ggctgtcgtg
     2761 ggagagcggc cagcgcgtgg tgtgggagta ctgggacggc cgcgactggc gcacgctgtc
     2821 ggtcaacgac gagacccagg gcttcaccag cagcgggttc gtgttcttca acgcgtccga
     2881 cgactgggtg atctccagca agttcaccga ggagcggttc tggctgcgcg cccggctgga
     2941 gcagggcggc tacgtcaagc cgccgcgggt ccggatgatc gtcaccaacg cgatcgacgc
     3001 gttcaaccag gagacgatcg ccgacgagat cctcggcagc tccgacggct cgccgctgca
     3061 gcagttcaag ttcctgcgca gcccgctgct cgaggacgag gtgatcgagg tccgcgagcg
     3121 ccagccgccg ccggccgacg aggtcgccga cctggccgcc gatccggtgc gcaaggtgca
     3181 gcccgacaac ccgcagaaca acgaggtctg ggtgcgctgg aagcgggtcg acagcttctt
     3241 cgcgtccggg ccgcgcagcc ggcactacac gctcgactac gtgtcgggca cggtgacgtt
     3301 cggcgacggc cggcgcggcg cggtgccgcc cgaggccaag gacaacatcc acacccggcc
     3361 gtaccggatc ggcggcggcg cgctgggcaa cgtcaacgcc cacacgctga cctcgctcgg
     3421 ccgcgcgctg gcctacatcg aatcggtgac caacccgctg ccggccaccg gcggcgccga
     3481 ccgcgagacc atcgacgagg ccaaggaccg cgcgccgtac acgatcaaga gccgcgaccg
     3541 cgcggtcacc tccgaggact acgagaccct ggcgctacgc gcgtcgacca cgctggcccg
     3601 cgcgcgctgc gtcccggacc gctccaaccg cggccacgtc acgctcgcgc tggtgcccaa
     3661 gggcgagctg cgcggcgagg agttcagccg ccggctggtg ccgtccaacg aggtcctgcg
     3721 ctacgtcaag cgctacctcg acgagcgcaa gctggtcggc accgtgctca acgtggtgcg
     3781 gccgcgctac aaggacctgt cgctgcgcgt ggtgctgatc cgccgcacgg tcggcacctc
     3841 ggaccggctg cgcaaggaca tcgagcacaa gctgcgcaag ttcctccact cgctgaccgg
     3901 cgggcgcgac ggcaagggct gggagttcgg ccggccggtg ctcaaggccg agctgatcca
     3961 cctggtcgag gaggtgcccg gcgtcgaggg cgtcgacgcc ctcgagatcc gcgacgagct
     4021 gcgcaacgtc ggcgtcgagc acctccggct ggaggacgac gagctgccct tcctggtcca
     4081 cgtcaacgtc gcggagaagg tccgcgacga catcaaataa cccgcacgac catggccacc
     4141 gaactgctca accgccgcgt gatccgcgtc cccaacgtgc tcggccgctc ggtcaccaag
     4201 gcgcggatcc tgctcgagga cgccgggttc aagaagatcg tcacgctgta ccgcgagagc
     4261 tacgaggacc gcgacacggt gctcgaccag aagccgccgc gcacccagat ggtgtacgag
     4321 gacaccgagg tcacgatctg ggtcgcccgg cgcggctacc tcgagaacct gccggcgatc
     4381 taccggcgga gcgacgcggt cggccgcaac ctggtgcgcg agctgtgctt cgtgttcgaa
     4441 cacatgttcg actcggtcga ggtcaacctc accgacggct ggcggttcta cgatccgcac
     4501 gtcgcgccgc tggacttcct caactggctg tcgcgctgga ccgcgttctc gctcgacctc
     4561 gactggccgg agccccagaa gcgggcgctg atcaagcgcg cggtcgacct gtaccggatc
     4621 cggggcacca agcgcggcct ggcgctgttc ctccggctgt tcaccggcca cgagcccgac
     4681 atcgaggaga acacctggcc gttcaagggg ttccgggtcg agggcgagga cgcggagagc
     4741 ggcgcgcggg tcgggctcga ctcggtgatc atgccgccgg tcgatctcgc gcactgcttc
     4801 atcgtgacga tgccgatgaa gtacgaggac gtgaccaccg acatggtgat ccgcatccac
     4861 cagatcatcc aggccgagaa gcctgcgcac acgcactatt acctgcgctt cgccgaggag
     4921 aagggcgagg tcgagctccg cgagttcttc gcgatcggcc tgcggtccgg gatcggcatc
     4981 ggcgcggaag tcgtccagtc catcgacgag gaaaagtcat gagtgatgca ccgcagcagg
     5041 gattcaagcg catcaacttc ttcaaggggt tcctcaccac cgagaaggac tggaacgacg
     5101 ccgagcgcta ccacatcgac aagcggcgcc tgcacaaccg catgctgcac tcgcctggca
     5161 tcgtctacgg cttcgccggc gatctccggg tcaacgcccg cgcgcgcggc gacctgtcgg
     5221 tcgaggtcca gcccggctac gcgatcgacg gcatgggcaa cgacctgatg atcttcgacg
     5281 cgacgatccg caacatcaac ctcgaagagt tccggctgcc ccagacggtc tacgtggtgc
     5341 tgcgctacta cgaggagctc accgacttca tcgcgtacaa ggagaacctc gagtacaagg
     5401 gccaccgccg cgtgctcgag tcgtgccggg tcgagctgtc gcagaccgag cccgacatcg
     5461 cgcgcgaggt cgagctcgcc cggatctacc tcgagaaggg cgtgcagcgc gtccgcgacg
     5521 cgcgcgatcc ggcgaacccg gcgcccaacg agatcgacat gcgctacacg ccgcgcggcg
     5581 gcctgtcggg ctcgtcgctg ccgccccacc tgcggctccg gctcgagctg ctgctgctcg
     5641 cgatccggcg cggcggcctc gagtacaccc ggcgcggcgt gatcgcggcg cacgacctgg
     5701 tgcaggcgtg caacaccgcg ctgatgctgc tctacgccaa cctgctcgac ctccgcaaca
     5761 tcttcgacat cttcaagctg atcatcgact gcgagggcga gctcgcgctc gacgtcgacg
     5821 tccaccaccc gacgatcgcg cagaagaagg agttctcgga gtaccgccgg caggtcgaga
     5881 tcggccgggg cttgctcgcc gagggcaaga acaacatgga ggcgttccag aacctgctcg
     5941 cgttccagaa caaggcctcc gagatcgcgc agtccgcggt cgccggcgag aagccgcaga
     6001 tcgaggcgcc gcccgagaag aaggacgccg cccccaaggt cgccgacatc aaggactggg
     6061 agggcgtcaa gaccatgccg cagcccaagc cgtcgatgga cctcgagggc ctgacctggg
     6121 tgctggtcga cgagatcgag atcctcaaca aggagtccga ggacaagcac ctgttccaga
     6181 tcaaggaagc caaggacagc taccggtcgc ggcagaagct caagtacccc gacggcacca
     6241 cggtcgagga caccggccgc gctcacgtcg acggctacag cgagttcaag ctgtcgaaca
     6301 tcacgccggg ccgcccggtc gtggtcctgc gccgcatgga ctacgtctac ggcgactacg
     6361 agctcgagct cagcgtcaac ggcaagaagg tcggcatcgt gtcatgcgtc ggcaccgacc
     6421 gcgtccaccg ctggcgcaac tggccggcgc tgatcggcgt cgagaacgtc accacggaga
     6481 acctgatcgt ccgccagcag tcggtgaccg cgggccgcga catcaacatg ttccacatct
     6541 gggtctacca gccgaagtag ctgggggcgc gggtcgggtc gcgcggccct ggacctgcgg
     6601 gcgctgcgct cgctggttta tttcagccgc cgacgatgac gttggtcgag ccctcgatca
     6661 tctggcccga gccgccgcag tgctggtcct gatcgcccat gcggtgggcg gccttgccgt
     6721 tgatgaagac ggtgccgctg ccgaccttgg cggtccaggt gttggggccg cagcacgcca
     6781 tgtggatccc ggtgtcgtcg acccggatcg cgggcagctt gttcacgttc acgtcggtcg
     6841 atccgatgat ggcgggaccg accgcggggt gcgggcaggc ggggcagccg tgggcgtccg
     6901 ccgggacgtt ggacttatct ccgagtcgac actggccggg catgggagct cctggagagg
     6961 gacgatcagg tgaagccgcc gggggtcatg gtgccaacgc cgccgaccac cgggccaacc
     7021 ggcacgtagg gcggggcgaa cgtcgggatc gggccggtgc ccaggacgtt ggtgaccatc
     7081 gtgctcacct tccacagatt gtaggacttc tcgaacgcgt cggcgatcga ctcgaacagc
     7141 tcctggtgga acggcgcgtt gggatcgccg agctggccga ccatctgcat cttgagcacg
     7201 ttgcacgaga tcgacatcgg gacctgggtc agggccgcga acgggcacgg cacgttgggc
     7261 gccggcggcg cgaccgggct cggcaccgcg gcgaacgccg ggtaccacgg catgccggtg
     7321 accttgaccg tcgcggtgaa gctcagccac gccgtgccga tcacgttggc gatcacgttg
     7381 gagtacttca tctccatcgg ggtcgacttg ggcgccgacg ccaggatcag cggcgtccac
     7441 ggcggcccga cgatctggcc gcccatcgcg gtcaccgcgt tgatcaccac gccgaccatc
     7501 gtggcggcgg tctgccactg gctccacgcc gagcagatcg ccgagcacgt gccgtcgatg
     7561 aacgcgccga tcttgccgat gtgcatcttc tgcgtatcgg tgtggtactt gttcagcgac
     7621 gcgggctgga acagcggcgg catcccgggg gccgacgcct tctcgtccgg cttgaacgcc
     7681 cggccgtagt gatccgcggc gctcccctgc ggatccttcc agttctgcgg gaccttcagg
     7741 ctgaacgagg tgaacttcaa cctcgctaac tgctgcatgg cgctggcttg cggggcgggc
     7801 atgggctcct ctgggctacc aatatacgga ccgagcgggc cgcaatttcc ctggcatcgt
     7861 cacatttttc gcgcccttac gtagtgaatc gaatagcttg cgagggtccg ggcgtcgcgc
     7921 cgcagcgtcc agggcagctc ggcctcgtcc tcgatctcgt agcgggcggc gagcccggtg
     7981 ccggcgcgca gcagcgcggt gagctcggcc gccgggtccg tcgcatccat catcgcgcac
     8041 caccccgcgc cccatcccag ccgctcgtgg tcggccatca ccgagctctg ccaggcgtac
     8101 ggcgacgaca cgatgatctc gccgcccggc tggcacaggc cgtcgagcac cgccagcagc
     8161 tggcgcgggc tcgccaccga gtcgagcagg ttgagcgcga ccacccggcc gaacaccccg
     8221 gggacgagcg gcggatcgag cgcgtcgccg cacaccagca ggcggcggtc ggcgggcacc
     8281 gcgcgatcgc cggcggcgac ctgggccgcg gcatagcgcc ggccgacgac ccggcgcgca
     8341 tactcgacgc gctcgcc
//
LOCUS       DASVOR010002079         7990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_23215,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002079 DASVOR010000000
VERSION     DASVOR010002079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7990
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23215"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..901
                     /gene="secF"
                     /locus_tag="VF516_38620"
     CDS             <1..901
                     /gene="secF"
                     /locus_tag="VF516_38620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEX8113729.1"
                     /translation="EFQKKFADRQIIKASWSGDNLTVRTKKLITNAEAKPVFAAAGLD
                     LKPWTEQEAARSSTPDASSGDFTEEFSMQGLAEQFKQLFEKQLPDTDVIVAQSYGVGA
                     KAGDKLRDDAAKSIIYSIFLIMLYLAFRFDIRYAPGAAFATIHDAVMVIGVFSLTWTD
                     VSLVSVAGLLTVMGYSTNDTVIVFDRIRENQAKLKDKKIERIIDISINEMLVRTILTS
                     STVFATTLIMNIFGTGEVRNFAFAMNVGVIVGTYSSIFLAAPIFMWISRKWYSGPAPA
                     RRRPSLVSSVPGPDTPSGAESEG"
     gene            complement(902..1972)
                     /locus_tag="VF516_38625"
     CDS             complement(902..1972)
                     /locus_tag="VF516_38625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113730.1"
                     /translation="MTRQFSRVGAAVRLATASTYPGFYLRDNLASSGSVPALPPYNQC
                     PDIIGTTTPIPDPQVVLSTLDSWDRIYRTEPQPGVNYYYVRGLNGLPSGAFDGKLSLF
                     WTPAELLLFPSLWKNHPLATASGSETVSVSAPAGHIGVGVDPFVLNWPSPASRGGATF
                     TSFVAQNLAAPIPTISSWIEMSQLMTQQLNFGWRNGIAFDPIANNGMMLHRMGLTVPG
                     TVGDSATLQLIVTANGFVGDTIALLADRYTAEQKAIQLAPTRITQDGQSIGIQINLDP
                     GFHANLTVQYWNTSDNVPAAGSTITVTASYVVPKANLEHAITAGVIDSHLSNTVARQI
                     GVGPQPVAPVGAVTFLAGPSGP"
     gene            complement(2007..3134)
                     /locus_tag="VF516_38630"
     CDS             complement(2007..3134)
                     /locus_tag="VF516_38630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113731.1"
                     /translation="MLIQARTDHSFVTRGPIALPRPRAALAASAFNGIYMRPNSSDDG
                     SVPALSPYTSCPDIWLAGTSAVPNYQTALATSQSYASSSSNNIYSGQTNILYVRGRNG
                     ATANKTVNVTLYYAPSGVIQSPSQWQGNVIQTDNGQQQGYISNLAPNAIGVCDATFVW
                     KNPVPPPPGSDHYCLFAQFNDSNNSNPFPNVETALDMGALIMNNLGWGWRNTSMINAT
                     ATWSYTEPLTIPSNYPTTQAYSIFIAPVGYVGWDAEFYASRADAKGNPIRLGRTKVTA
                     DGVLLGVGQAVLDPGFNALMTVNMYSPNGQPPPPGATVPMSCNYAAQPRSGLYEALDR
                     GLVDWEFMARVRRSIPDLGVTPTAWIPQGYYNWTVINPAHR"
     gene            complement(3171..6632)
                     /locus_tag="VF516_38635"
     CDS             complement(3171..6632)
                     /locus_tag="VF516_38635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113732.1"
                     /translation="MQQYRIGAAVHGGGDIAALQNGAGGVEAFTIATDTQVWDFYPDT
                     ASDTGVSAVATPLTASAVTAGRDRRGRIVLFAADHLQINYVVENAPGSSSRWGAMQVA
                     TLPLPPGAITIAGIYTGEIGGALYVGALIETQSISPGKTYAFVYSVWDDNPGAFTATT
                     MMVSTLNCVWSGHSASTAQFTCLDVVYLGYDITTRQIVRYPFSSTFSSLSVATTLDAA
                     SNNRYFAVLNDGNLYEMVGGSGGKPFSWAQLTQQQSYRSVAVTLDTAGATHLFALGSN
                     GDLEHLWARKDAPTAFSTPAVVQSNVALMAVTALDQGDVQLFAIGTAQATLIQLIWQA
                     GAGNWQVTPVEVPTSGQVEEFVSYSTDLQIRDAAGAPVPKAVVQVRASSQTQITVNGG
                     FYTVDPSTPATLTASAAGTLTITQQTDTLVIPTLQFEIASVTTAPLAVQQFAGVQQQL
                     ATVTGDQLKAAKKADGNYLLPDKYRNDKSTTDAVASACNQAMAIAAMTKRPAPLAATR
                     HGHKPGVGTIPAGSLADLHRIVVEPGRDGPHWQIDFSTGQARFRTLTADEARALMVEK
                     LARHPTPDRAGGFLDWIGSIGDLIEGIANKIVDVIDTVVTTVGNAIHAVLTFVIDGIT
                     YVFNAVVNVIEQAFDLVQVVFARVAAFFSDLFQWLGFLFAWGDILRTHKALVYTANQF
                     LGFLPLAIGGVQRYIDNGIATVQSQIPAIFDRLVAAVGGASLGGYVDAKTPSEPTFSS
                     SNANNIVLNGTIDNAAGARYTPIDPTTMGPFDSVRQLIVQLVDAVQGQPAFQQALDYM
                     TNLGGNPDQIFVQLASALLRVVQGLAQAMLSGAQIVVDAVLGLVQGFMAGIQSTLNES
                     WDIPFVTAFYGWLTDGAPLTLLDLLCLILAIPATVLFKATQHAAPFPDDASVAAFEAS
                     FTAQTMLANSGLGTHQTTALARSAAPAVGDPKPAWQVLLSAAGFLSFAGYGLLSAAMD
                     VKPMTGGHVVDPLVKTLTKIALALEIIAQAVACPWIYGASAPNCTDAEGAGGVFWIYE
                     TVGVALDAGFTWYDSAFPENNDTYWGIGIAELYGVGHAIVTGVLGSKLSGVGLASKIV
                     LLIPECCKFLRLPQIETASEGWSLIGIAALDGLCIPASGLLSFVDSITPAAHTEHAAP
                     LRIQGAV"
     gene            complement(7142..7621)
                     /locus_tag="VF516_38640"
     CDS             complement(7142..7621)
                     /locus_tag="VF516_38640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113733.1"
                     /translation="MTDAPRTPERPTERTIEPPAEAPGPDEPVPRLPRGRGMRFSRPE
                     LFRVAGLAILLVFLLVTQRPCADAVSRFVTSFGDNGSAAAPLPRPGTVDLPAGGAGSD
                     LGSDLDRYEQLRPGMSEAEIKAAIERARARAHSGSGERSSGDAPTPSPPPPSGPPPR"
     gene            complement(7640..>7990)
                     /locus_tag="VF516_38645"
     CDS             complement(7640..>7990)
                     /locus_tag="VF516_38645"
                     /inference="COORDINATES: protein motif:HMM:NF037628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113734.1"
                     /translation="VATGREVDAEVRLADVDERLAEELAGLGPFGQDNPSPVLVTRNV
                     RVSAARRVGDGSHLKLTLEDDRSTTRTAIGFQLGDREVDVGARVDLAFLPTVSTWQGR
                     RSAELELSDLAIVS"
BASE COUNT         1221 a         2597 c         2870 g         1302 t
ORIGIN      
        1 cgagttccag aagaagttcg ccgaccgcca gatcatcaag gcgagctggt ccggcgacaa
       61 cctcaccgtc cggaccaaga agctgatcac gaacgccgag gccaagccgg tgttcgcggc
      121 cgccggcctg gacctcaagc cgtggaccga gcaggaggcc gcgcgctcca gcacgcccga
      181 cgcgagcagc ggggacttca ccgaggagtt ctcgatgcag gggctcgccg agcagttcaa
      241 gcagctgttc gagaagcagc tccccgacac cgacgtgatc gtcgcccaga gctacggcgt
      301 cggcgccaag gccggcgaca agctgcgcga cgacgccgcc aagtcgatca tctactcgat
      361 cttcctgatc atgctgtacc tggcgttccg gttcgacatc cggtacgcgc cgggggcagc
      421 gttcgcgacc atccacgacg cggtcatggt gatcggcgtg ttctcgctga cctggaccga
      481 cgtctcgctg gtctcggtcg ccggcctgct caccgtgatg gggtactcga ccaacgacac
      541 ggtgatcgtg ttcgaccgga tccgcgagaa ccaggccaag ctcaaggaca agaagatcga
      601 gcgcatcatc gacatctcga tcaacgagat gctggtgcgg accatcctga cctcgtcgac
      661 ggtgttcgcg accacgctga tcatgaacat cttcggcacc ggcgaggtcc gcaacttcgc
      721 gttcgcgatg aacgtcggcg tgatcgtcgg gacctactcg tcgatcttcc tggccgcgcc
      781 gatcttcatg tggatctcgc gcaagtggta ctccgggccg gcgcctgcgc gccgccgtcc
      841 gtcgctggtc agctcggtcc cgggccccga cacgccgtcg ggggccgagt ccgagggctg
      901 atcagggtcc cgacggtccg gcgaggaagg tcaccgcgcc gaccggtgcg accggctgcg
      961 gaccgacccc gatctggcgc gcgacggtgt tgctgaggtg cgagtcgatg accccggcgg
     1021 tgatcgcgtg ctcgaggttg gccttgggca ccacgtagct cgcggtcacc gtgatcgtcg
     1081 agcccgccgc cggcacgttg tcgctcgtgt tccagtactg cacggtcagg ttggcatgga
     1141 acccgggatc gaggttgatc tggatcccga tcgactggcc gtcctgggtg atccgcgtcg
     1201 gcgcgagctg gatcgccttc tgctccgcgg tgtagcgatc ggcgagcagc gcgatggtat
     1261 cgccgacgaa gccgttggcg gtcacgatca gctgcagggt cgccgagtcg ccgaccgtgc
     1321 cggggacggt gagccccatc cggtgcagca tcatcccgtt gttggcgatc ggatcgaacg
     1381 cgatcccgtt gcgccagccg aagttgagct gctgggtcat cagctggctc atctcgatcc
     1441 agctgctgat cgtcgggatc ggcgcggcga ggttctgcgc gacgaacgag gtgaacgtgg
     1501 cgccgccgcg tgacgcaggc gacggccagt tcagcacgaa cgggtcgacg ccgaccccga
     1561 tgtggccggc cggcgcgctg accgacacgg tctcgctgcc cgatgcggtg gccagcgggt
     1621 ggttcttcca cagcgagggg aacagcagca gctccgccgg ggtccagaac agcgagagct
     1681 tgccgtcgaa cgcaccgctc ggcaggccgt tgaggccgcg cacgtagtag tagttgacgc
     1741 cgggctgcgg ctcggtgcga tagatccggt cccagctgtc gagcgtgctc agcacgacct
     1801 gaggatccgg gatcggagtc gtggtcccga tgatatcggg gcactggttg tacggcggga
     1861 gcgccggcac gctgccgctg gaggccaggt tgtcgcgcag gtagaacccc gggtaggtcg
     1921 aggcagtggc gaggcggacc gccgcaccga cacgagaaaa ttgacgggtc atgttgtctc
     1981 ctgaatggaa tcgtgggttg tcgagctcag cggtgggcgg gattgatgac ggtccagttg
     2041 tagtatccct gtgggatcca cgcggtcggc gtgacgccga gatcggggat gctgcgccgg
     2101 acccgcgcca tgaactccca gtcgacgagg ccgcggtcga gcgcctcgta cagccccgat
     2161 ctgggctgcg ccgcatagtt gcacgacatc gggacggtgg cgccgggggg cggcggctgg
     2221 ccgttgggcg agtacatgtt gaccgtcatc agggcgttga acccggggtc gagcacggcc
     2281 tggccgacgc cgaggagcac gccgtcggcc gtcaccttgg tgcgccccag ccggatcggg
     2341 ttgcccttgg cgtcggcgcg cgacgcgtag aactcggcat cccacccgac gtagccgacc
     2401 ggtgcgatga agatcgagta ggcctgcgtc gtcgggtagt tgctcgggat ggtcagcggc
     2461 tcggtgtacg accaggttgc cgtcgcgttg atcatcgagg tgttgcgcca gccccacccg
     2521 aggttgttca tgatcagcgc acccatgtcg agcgcggtct ccacgttggg gaatggattg
     2581 ctgttgttgg agtcgttgaa ctgggcgaac aggcagtagt ggtcgctgcc cggcggcggc
     2641 ggcaccgggt tcttccacac gaacgtcgcg tcgcacacgc cgatcgcgtt gggcgcgagg
     2701 ttggagatgt agccctgctg ctggccgttg tcggtctgga tcacgttgcc ctgccactgc
     2761 gacggcgact ggatgacgcc cgacggcgcg tagtacagcg tcacgttgac cgtcttgttg
     2821 gcggtcgcgc cgttgcggcc gcgcacgtac aggatgttgg tctgcccgct gtagatgttg
     2881 ttgctcgacg acgacgcgta gctctgcgag gtcgccagcg cggtctggta gttcgggacc
     2941 gccgaggtcc cggcgagcca gatgtcggga cacgacgtgt agggcgacag cgccggcacc
     3001 gagccgtcgt cgctggaatt cggccgcatg tagatgccgt tgaacgcgct ggcggcgagc
     3061 gcggcccggg gccgcggcag cgcgatcggg ccgcgggtga cgaacgaatg atcggtcctt
     3121 gcctgaatga gcatgtttca cctctggaat gagaatgttg aaggacaggg tcagactgcg
     3181 ccctggatgc gcagcggcgc ggcgtgctcg gtgtgcgcgg ctggcgtgat cgagtcgacg
     3241 aacgacagca gccccgacgc ggggatgcac aggccgtcga gcgcggcgat cccgatcagc
     3301 gaccagccct cgcttgcggt ctcgatctgc ggcagccgca ggaacttgca gcactcgggg
     3361 atcagcagca cgatcttcga ggccagtccg accccgctca gcttgctgcc gagcaccccg
     3421 gtgacgatcg cgtgaccgac cccgtagagc tcggcgatgc cgatccccca gtaggtatcg
     3481 ttgttctcgg ggaacgcgct gtcgtaccag gtgaacccgg catcgagcgc cacgccgacg
     3541 gtctcgtaga tccagaacac gccgccggca ccctcggcgt ccgtgcagtt cggggcgctc
     3601 gcgccgtaga tccacggaca cgcgacggcc tgcgcgatga tctccagcgc gagcgcgatc
     3661 ttggtcagcg tcttgaccag cggatcgacg acgtggccgc cggtcatcgg cttgacgtcc
     3721 atcgcggcgc tgagcaagcc gtagccggca aacgacagga acccggcggc cgacaacagg
     3781 acttgccacg ccggcttggg atcgccgacc gcgggcgcgg cgctccgcgc cagggcggtg
     3841 gtctggtgcg tccccagacc ggagttggcg agcatggtct gcgcggtgaa cgacgcctcg
     3901 aacgcggcga cgctggcatc gtcggggaac ggcgcggcgt gctgggtcgc cttgaacagc
     3961 acggtcgccg ggatcgcgag gatcaggcac agcagatcga gcagcgtgag cggcgcgccg
     4021 tcggtcagcc agccgtagaa cgcggtgacg aacgggatgt cccatgactc gttgagcgtc
     4081 gactggatac cggccatgaa gccctgcacg agcccgagca cggcatcgac cacgatctgg
     4141 gctccggaca gcatggcctg ggccaggccc tggacgaccc gcagcagcgc ggacgccagc
     4201 tggacgaaga tctggtccgg gttaccgccg aggttggtca tgtagtccag cgcctgctgg
     4261 aacgcgggct ggccctggac cgcgtcgacc agctgcacga tcagctggcg caccgaatcg
     4321 aacggcccca tcgtggtcgg atcgatcggc gtgtagcgag ctccggcggc gttgtcgatc
     4381 gtcccgttga gcacgatgtt gttggcgttc gacgacgaga aagtcggctc gctcggggtc
     4441 ttggcgtcga cgtagccgcc caggctggcg ccaccgaccg cggcgaccag gcggtcgaag
     4501 atcgccggga tctgactctg gaccgtggcg atcccgttgt cgatgtagcg ctggaccccg
     4561 ccgatcgcga gcggcaggaa cccgaggaac tggttcgcgg tgtacaccag cgccttgtgg
     4621 gtgcgcagga tgtcgcccca ggcgaacagg aagcccagcc actggaacag gtcggagaag
     4681 aacgccgcga cccgcgcgaa cacgacctgc acgaggtcga acgcctgctc gatcacgttg
     4741 accacggcgt tgaacacgta cgtgatgccg tcgatcacga acgtgagcac cgcgtggatc
     4801 gcattgccca ccgtggtgac cacggtgtcg atcacgtcga cgatcttgtt ggcgatgccc
     4861 tcgatgagat cgccgatcga gccgatccag tccaggaacc cgccggcccg gtccggcgtg
     4921 gggtggcgcg ccagcttctc gaccatcaac gcgcgggcct cgtccgcggt cagcgtgcgg
     4981 aaccgggcct ggccggtcga gaagtcgatc tgccagtgcg ggccgtcgcg gccgggctcg
     5041 accacgatgc gatgcaggtc ggcgaggctg ccggccggga tcgtgccgac cccgggcttg
     5101 tgaccgtggc gggtcgccgc gagtggtgcc gggcgcttcg tcatcgcggc gatcgccatc
     5161 gcctggttgc acgcgctggc cacggcgtcg gtggtcgact tgtcgttccg gtacttgtcg
     5221 ggcagcaggt agttgccgtc ggccttcttg gcggccttga gctggtcgcc ggtgacggtg
     5281 gcgagctgct gctggacgcc ggcgaactgc tggaccgcca gcggcgcggt ggtcaccgag
     5341 gcgatctcga actggagcgt cgggatcacc agcgtgtcgg tctgctgggt gatggtcagc
     5401 gtgccggccg ccgacgcggt cagcgtggcc ggggtgctgg gatcgacggt gtagaacccg
     5461 ccgttgaccg tgatctgggt ctgcgacgat gcgcggacct ggaccacggc cttgggcacc
     5521 ggcgcgcccg ccgcgtcgcg gatctgcaga tcggtcgagt acgacacgaa ctcctcgacc
     5581 tggcccgagg tcggaacctc gaccggcgtg acctgccagt tgcccgcccc ggcctgccag
     5641 atcagctgga tcagcgtcgc ctgcgcggtg ccgatcgcga acagctggac gtcgccctga
     5701 tcgagcgcgg tcacggccat cagcgcgacg ttgctctgga ccaccgccgg ggtcgagaac
     5761 gcggtcggcg cgtccttgcg cgcccacagg tgctcgaggt cgccgttcga gcccagcgcg
     5821 aacagatggg tcgcgccggc ggtgtcgagc gtcaccgcga cgctgcggta gctctgctgc
     5881 tgggtcagct gggcccagct gaacggcttg ccgccgctgc cgccgaccat ctcgtacagg
     5941 ttgccgtcgt tcagcaccgc gaagtagcgg ttgttggacg ccgcgtcgag cgtggtcgcg
     6001 accgacagcg acgagaacgt cgacgagaac gggtagcgca cgatctggcg agtcgtgatg
     6061 tcgtagccga ggtagacgac atcgaggcag gtgaactgcg cggtgcttgc gctgtggccc
     6121 gaccacacgc agttcagggt cgacaccatc atcgtggtgg cggtgaacgc gcccgggttg
     6181 tcgtcccaca cgctgtacac gaacgcgtag gtcttgcccg gactgatgct ctgggtctcg
     6241 atcagtgcgc cgacgtacag cgcgccgccg atctccccgg tatagatccc ggcgatcgtg
     6301 atcgcgccag gcggcagcgg tagcgtggcg acctgcatcg cgccccaccg gctcgacgag
     6361 cccggcgcgt tctcgacgac gtagttgatc tggaggtgat cggccgcgaa cagcacgatc
     6421 cgcccgcgtc ggtcgcgccc ggcggtcacc gcgctcgcgg tcagcggcgt ggcgaccgcg
     6481 ctgaccccgg tatcgctcgc ggtgtcagga tagaagtccc agacctgcgt atcggtggcg
     6541 atggtgaacg cctcgacccc gccggcgccg ttctggagcg ccgcgatgtc tccgccgccg
     6601 tggaccgccg cgccgatccg gtactgttgc atgaacttgg tctcggtcag aagctgcggt
     6661 ccgctcgatt gcggcgcgtg cggagcgcgc ctgcgttttg acgatgcgac gaccggactt
     6721 gtgtgcccca tggaatctcc tttcgtcatc cagacccacg ccacgttatt cctggtgatt
     6781 tgctatctcc attacgcacg attactggcg atttcgtgta atgcgcggtg aatgcaccgg
     6841 tgcttccagc tgcggattcg ccggcattac atggaatgcg cggcgagcgc gtgaatgctc
     6901 ggcagcggat tcgtcggcaa ttcatgtaat gcgcggcgag cgcgcgaatg cctcggcggc
     6961 ggattcgtcg gcattacatg gaatgcgcgg cgagcgcatc ggtgcttcca gctgcgcatt
     7021 cgctgcgcat tccatgtaat gcgcagcgag cccacgggtg acccggctgc aggttcgccg
     7081 accggtcgac gcccggaccc agcagtgtta cgaggacctc gcgctcgccc tcgggcgccg
     7141 tctaccgtgg cggcggcccc gacggcggcg ggggtgacgg cgtcggagcg tctccgctgg
     7201 agcgctcgcc gctgccgctg tgggcacgag cgcgggcgcg ctcgatcgcg gccttgatct
     7261 cggcctccga catgcccggc cgcagctgct cgtagcggtc gaggtcgctg ccgagatcgc
     7321 tgccggcgcc gccggccggc aggtcgacgg tgcccggccg gggcagtggc gcggctgccg
     7381 agccgttgtc gccgaagctc gtgacgaagc gggacaccgc gtcggcgcac ggccgctggg
     7441 tcaccagcag gaacacgagc aggatcgcca gcccggcgac gcggaacagc tcggggcgcg
     7501 agaaccgcat gccgcggccg cgcggcaggc ggggcacggg ctcgtcgggg ccgggagcct
     7561 cggccggagg ctcgatcgtc cgctctgtcg ggcgctccgg cgtgcgcggc gcgtcggtca
     7621 cgcccgtggg atagcgcggc tacgacacga tcgccagatc ggacagctcg agctcggcgc
     7681 tgcgccggcc ctgccaggtc gacaccgtgg gcaggaacgc gaggtcgacc cgcgcgccga
     7741 cgtcgacctc gcggtcgccg agctggaacc cgatcgcggt gcgggtggtg gagcgatcgt
     7801 cctcgagcgt gagcttgagg tgcgagccat cgccgacccg gcgcgcggcg gagacccgga
     7861 cgttgcgggt gaccagcacc ggcgaggggt tgtcctggcc gaacggaccc aggccggcga
     7921 gctcctcggc gagccgctcg tcgacatcgg cgagccggac ctcggcgtcg acctcgcgcc
     7981 cggtggcgac
//
LOCUS       DASVOR010002080         1776 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_343003,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002080 DASVOR010000000
VERSION     DASVOR010002080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343003"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VF516_38650"
     CDS             <1..378
                     /locus_tag="VF516_38650"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8113735.1"
                     /translation="LAVFAASDAITLHCPLTPETRGMIDAAAFAAMRPGALLINVARG
                     AVIDRAVLVAALAGDRLGGVGLDVHWDEPADPADPIYTDPRVVALPHLGGSTAEAFGR
                     IADVVVDNLGRLTRGEPLRHRVA"
     gene            646..>1776
                     /locus_tag="VF516_38655"
     CDS             646..>1776
                     /locus_tag="VF516_38655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113736.1"
                     /translation="MLRGRQLGDFVLRECIGEGAFAAVYRCEQPLLGREAVVKVLHQR
                     LRNSEVVFQRFMREARLASRLDHPYAAHVYAFGVEPDDGLAWIAMELVHGTSLARWLS
                     EHGTMPLDMLVAFFEQVAEIVQNAHDHGIVHRDLKPSNVMIIEYAGKIFPKLLDFGIA
                     KLVDDAANGGMPSGEFSPPTHPGAVIPGGLVTGEFDDTLSPAPMGSGDIQLTQANATL
                     GSPPYMSPEQWCDPLSVGPRSDLYALGVMAYEALTGRQPFTGPTIAAIGEQHCNAEVP
                     PVGESYPPTLDRVFQRALAKHPGDRPASAIDFARELRAAAFPPPEASLAIVTLPPIRR
                     RMRSIAVGAAAAILGGVAAYFGAASLLASNHPPLRPALADPPP"
BASE COUNT          242 a          661 c          629 g          244 t
ORIGIN      
        1 ctggcggtgt tcgccgccag cgacgcgatc accctccatt gcccgctgac ccccgagacc
       61 cgcggcatga tcgacgccgc ggcgttcgcc gcgatgcggc cgggcgccct gctgatcaac
      121 gtggcgcgcg gcgcggtgat cgatcgggcg gtgctggtgg cggcgctcgc cggcgaccgg
      181 ctcggcggcg tcgggctcga cgtccactgg gacgagcccg ccgatcccgc cgacccgatc
      241 tacaccgacc cgcgggtcgt ggcgctgccc cacctggggg gatcgaccgc cgaggcgttc
      301 ggccggatcg ccgatgtcgt ggtcgacaat ctgggccgac tcacgcgggg cgagccgctg
      361 cgccaccggg tcgcgtgagc ctggtccacc ggacgcggcc gatgccgcac cgaccaggcg
      421 gccggtgccg cgcaacgtcg caccgacgat ccggagggcg gcgcaccgcg tgaccggcat
      481 caaacctgcg agaatcaaca gcgttgtggg gagatgacag cgcacattcc ggtgctcggt
      541 cgccacaaca ggccgcaatc gctcgcgtcc caccgtgtta gtctgggcgc cgtggaagcc
      601 accggggacc ggtccgtcgc ccgacctggg accgagagcg acgccgtgct tcgcgggcgt
      661 cagctcgggg acttcgtgct ccgcgagtgt atcggagaag gcgcattcgc cgcggtctat
      721 cgctgcgagc aaccgttgct cggccgcgag gccgtggtca aggtgctgca ccagcggctg
      781 cgcaacagcg aggtggtgtt ccagcgcttc atgcgcgagg cccggctcgc gtcgcggctc
      841 gatcatccct acgccgcgca cgtctacgcg ttcggggtcg agcccgacga cggcctggcg
      901 tggatcgcga tggagctggt ccacgggacc tcgctcgcgc gctggctgag cgagcacggc
      961 acgatgccgc tcgacatgct ggtcgcgttc ttcgagcagg tcgccgagat cgtgcagaac
     1021 gcgcacgacc acgggatcgt gcaccgcgat ctcaagccgt cgaacgtgat gatcatcgag
     1081 tacgccggca agatcttccc caagctgctc gacttcggga tcgccaagct ggtcgacgac
     1141 gccgccaacg gcgggatgcc gagcggcgag ttctcgccgc cgacccaccc cggcgcggtg
     1201 atcccgggcg gcctcgtcac cggcgagttc gacgacacgc tgtcgccggc gccgatgggc
     1261 agcggcgaca tccagctgac ccaggccaac gcgacgctcg gctcgccgcc ctacatgtcg
     1321 cccgagcagt ggtgcgatcc gctgtcggtc ggcccgcgct cggacctgta cgcgctcggg
     1381 gtcatggcgt acgaggcgct gaccgggcgc cagccgttca ccggcccgac gatcgcggcg
     1441 atcggcgagc agcactgcaa cgccgaggtg ccgccggtcg gcgagtcgta cccgcccacg
     1501 ctggatcgcg tgttccagcg cgcgctcgcc aagcaccccg gcgatcgacc ggccagcgcg
     1561 atcgacttcg cgcgcgagct gcgcgccgcg gcgttcccac ctccggaggc cagcctcgcg
     1621 atcgtgacgc tgccgccgat ccggcggcgg atgcgctcga tcgcggtcgg cgccgcggcg
     1681 gcgatcctcg gcggcgtcgc cgcgtacttc ggcgcggcca gcctgctcgc aagcaaccac
     1741 ccgccgctgc ggccggcgct cgccgacccg cccccg
//
LOCUS       DASVOR010002081         2428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_197556,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002081 DASVOR010000000
VERSION     DASVOR010002081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197556"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1644)
                     /locus_tag="VF516_38660"
     CDS             complement(<1..1644)
                     /locus_tag="VF516_38660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8113737.1"
                     /translation="MDPPTIVVLGGSEAERARLVEQLAATGVAATAETYLPMAHGLPA
                     LLIMVGSEAAQLVSEARDFPALAEIPILAMVPAIPPTAVAEALAAGATDVVRTPVPSQ
                     LLAARCRNLCRLGRRDAIAAQTLARINEVLTADGDDAEALIQVLTITAGLLGFERASL
                     VAHIEGSDHAFVIAASDADPLQRFTVVIAEYPEVAESIRTLAPVLIDDVTRHPLTASI
                     AGLSARAVRGLAVFPVQWRGRALGAMLLRKRVPGVQHVGPKGIELGKLIASITAAHLR
                     HGAVLESLRDQGHRISRARYEAERRLRGIDSLKEHFEAGADGVVVLDDAGRILFVNRA
                     AERLTGFARDGLVGGELIDLVAADHRSQISDTVARVLAGVNVEAFDLQLSTTSGARVW
                     VSVSTSTVLAGSGAVILQFRDVTAERGLEQELRSTKEFLERLIDSAVDAIIAADLRGQ
                     IILFNQGSERLFGYRARDVIGKIPVWELYEEGGATQILRMLRSTSYGGVGRLEQTRRE
                     VRIASGEVVPVSMTASTLYERDREVATVGILTDLRERIRM"
     gene            2240..>2428
                     /locus_tag="VF516_38665"
     CDS             2240..>2428
                     /locus_tag="VF516_38665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113738.1"
                     /translation="MLFTTTTADRAVHPVQRPGALALRSVFFVGLALASLAGPPAAFA
                     QSVAGGASHAAAVAQAANQ"
BASE COUNT          361 a          872 c          871 g          324 t
ORIGIN      
        1 catgcggatc cgctcgcgca ggtcggtgag gatgccgacc gtggcgacct cgcgatcgcg
       61 ctcgtacagg gtcgacgcgg tcatgctgac cgggacgacc tcgccgctgg cgatccggac
      121 ctcgcggcgg gtctgctcga ggcggccgac cccgccgtac gaggtcgagc gcagcatgcg
      181 caggatctgc gtcgcgccgc cctcctcgta gagctcccac accgggatct tgccgatcac
      241 gtcgcgcgcg cgataaccga acagccgctc ggagccctgg ttgaacagga tgatctggcc
      301 gcgcaggtcg gcggcgatga tcgcgtcgac cgcggagtcg atcagccgct cgaggaactc
      361 cttggtcgac cgcagctcct gctcgaggcc gcgctcggcg gtgacgtcgc ggaactgcag
      421 gatcaccgcg ccgctgccgg cgagcaccgt cgaggtcgac accgagaccc acacccgcgc
      481 gcccgaggtc gtcgagagct ggaggtcgaa ggcctcgacg ttgacgccgg cgagcacacg
      541 cgccacggta tccgagatct gggagcgatg gtcggcggcg accaggtcga tcagctcgcc
      601 gccgaccagg ccgtcgcggg cgaacccggt cagccgctcg gcggcgcggt tgacgaacag
      661 gatccggccg gcgtcgtcga gcacgaccac gccgtcggcg ccggcctcga agtgctcctt
      721 gagcgagtcg atgccgcgca gccggcgctc ggcctcgtag cgcgcgcggg agatccggtg
      781 gccctggtcg cgcaggctct ccagcacggc gccgtggcgc aggtgggcgg cggtgatgct
      841 cgcgatcagc ttgccgagct cgatgccctt gggcccgacg tgctggaccc cgggcacgcg
      901 cttgcggagc agcatcgcgc ccagcgcgcg gccgcgccac tgcacgggga acaccgcgag
      961 gccgcgcacg gcgcgggccg acaggccggc gatcgacgcg gtcagcgggt ggcgggtgac
     1021 gtcgtcgatc aggaccggcg ccagcgtgcg gatcgactcg gcgacctcgg ggtactcggc
     1081 gatcaccacg gtgaagcgct gcagcggatc ggcgtcgctg gccgcgatca cgaacgcgtg
     1141 atccgagccc tcgatgtggg cgacgagtga cgcgcgctcg aacccgagca ggccggcggt
     1201 gatcgtcagc acctggatca gcgcctcggc atcgtcgccg tcggcggtca gcacctcgtt
     1261 gatccgcgcc agggtctggg cggcgatcgc gtcgcggcgg ccgagccggc acaggttgcg
     1321 gcagcgcgcc gcgagcagct gggacgggac cggggtccgc acgacgtcgg tcgctcccgc
     1381 cgccagcgcc tcggcgaccg cggtcggtgg gatcgccggg accatcgcga ggatcgggat
     1441 ctcggcgagc gcggggaagt cgcgggcctc cgacaccagc tgcgcggcct cgctgccgac
     1501 catgatcagc agcgccggca ggccgtgcgc catcggcagg taggtctcgg cggtcgccgc
     1561 gacgccggtt gccgcgagct gctcgaccag ccgagcgcgt tcggcctcgc tgcctccgag
     1621 cacgacgatc gttggcggat ccacgacgga tcaccgtacc acggggtcgg tggagggagc
     1681 gcgcttccgc tccggttgct ccagagcagc cgcgttggac atatccggga tacggcgcgc
     1741 ttccagcatc gcagcccgtc gcagccggag cgatcgccga ggcgggatgc gcgtgtcgtc
     1801 gtccgcgctg gcccctcgct gcatcgccat cccggggcgg tcgtgtcggc gaacggcgcc
     1861 gagcggccgc gcacacccgc ccggaacacg ctcacccgaa ccagcgcacg cgctcgcatc
     1921 atcacgggat cgcgccgagg tggcaggcaa gatccggcaa ggccaggacg cagcccctcg
     1981 ccgcacgcac gagcgcagtc gcacgcggtg atcgcgatcc gcgagcgcgg acgcggtccg
     2041 cagatgcgga ctcgatcgcg tcccgcaagt tggccaatgt ccgattcacg acctcgatag
     2101 cgagtccgca atgcggattt gcaatcgatc ctcgagccgg cacctcgctt gctcgacgag
     2161 ttcttcgacg acgacgcgat cgcgtcactg gcaatcacac aaacacacat ccaggcactc
     2221 gaaccagaag gaactttcca tgttgttcac gaccaccacc gccgatcggg cggtccatcc
     2281 cgtacagcgt cccggcgccc tcgcgctgcg gtcggtattc ttcgtcggtc ttgcgctggc
     2341 cagcctggca ggcccgccgg ccgcgttcgc acagagcgtc gcggggggcg ccagccacgc
     2401 cgccgcggta gctcaggcgg cgaaccag
//
LOCUS       DASVOR010002082         2086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_343015,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002082 DASVOR010000000
VERSION     DASVOR010002082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343015"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..780
                     /locus_tag="VF516_38670"
     CDS             283..780
                     /locus_tag="VF516_38670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113739.1"
                     /translation="MRFALALAIVIGIAAPARAEDLDGRGADKGTFGVGIIVGEPVGI
                     TAKLYLTDDRAIQAAVGSAFIGGGLQLHGDYVFHPYILQTRDSFVLPVYVGPGVRMID
                     YNNGRDSNAFAFGVRAVGGLLFDFKTVPLDAFFEIAPVLEYKFSSGAGFQLSLNVGAG
                     IRYYF"
     gene            842..>2086
                     /locus_tag="VF516_38675"
     CDS             842..>2086
                     /locus_tag="VF516_38675"
                     /inference="COORDINATES: protein motif:HMM:NF024676.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HEX8113740.1"
                     /translation="MPTKKGKDARPGKTARAPRGDDPASRGDRSKRLLDLVMILLRAR
                     MPVTYRDIHEQFPAYQTLNAEAGLRAFERDKADLLDLGVPIRYITPDEDDSLEDGGYV
                     IDLKRFKMPEVRLTPDEISALVLAASVAHAMPGGAYPKIVDLALKKLAFDLPELPDTP
                     TEFPRPAPVLVHFPERRITATGARADLAEIYATIEAATRHRKRVTLTYQAAATGMRSR
                     RDVDPYALIYRESAWLVVGWCHLRREVRTFRIDRIHEAVMAPRPKSPDFERPADFDVK
                     AYAQRSAWTFNTEPPEEIQLALAPEAAEVGNEDFGLTAVKRIDGDRTLVTFDCSNLEF
                     AATRILAAKGAIRLLRGNRLRARIHDELDAIEAHYPVLDDELEALRHHAHNGHDGANG
                     VVGLGAPEVAGPADAALPGGPGE"
BASE COUNT          310 a          786 c          707 g          283 t
ORIGIN      
        1 cgatcctcga gcgctgcgac ggcaaccagt ccgccgccgc ccgcctgctc gggatcagcc
       61 gcaacaagct cgcccgcctg ctcaaggcgt gagccccgcg gcggccggtg ccaggcgctg
      121 cgcggcggcg acccagatcc acacccggat ccgcatcccg gccacggtac cgcaggcggt
      181 gacgccgcca cagtgcgcgg ctggcgcgat cggctgcacg gcggcgctcc cgcggcccgt
      241 cgcggatttg cgcccgatcg aagcgccggg ctaggttgtc gcatgcgctt cgctcttgcc
      301 ctggcgatcg tgatcgggat cgccgcaccc gcccgcgccg aggacctcga tggccggggc
      361 gccgacaaag gcacgttcgg cgtcggcatc atcgtcggcg agccggtcgg gatcaccgcc
      421 aagctctacc tgaccgacga tcgcgcgatc caggcagcgg tcggatcggc gttcatcggc
      481 ggcggcctgc agctgcacgg cgactacgtc ttccacccct atatcctgca gacccgggac
      541 agcttcgtgc tcccggtcta cgtcggcccc ggtgtgcgga tgatcgacta caacaacggc
      601 cgcgactcga acgcgttcgc cttcggcgtg cgcgcggtcg gcggcctcct gttcgacttc
      661 aagaccgtgc cgctcgacgc cttcttcgag atcgcgccgg tgctggagta caagttcagc
      721 tccggcgcgg ggttccagct gtcgctcaac gtcggcgccg gcatccgcta ctacttctga
      781 ccgccgccca accctcgcgg cggtgcttcc gaatttcgcg gtcgtgcgct agaacggcgc
      841 cgtgcccacg aagaagggga aggacgcacg cccggggaag accgcgcgcg cgcctcgcgg
      901 cgacgatccg gccagccgcg gcgaccgcag caagcggctg ctcgacctcg tgatgatcct
      961 tctgcgcgcg cgcatgccgg tgacgtatcg cgacatccac gagcagttcc cggcctacca
     1021 gacgctcaac gccgaggccg ggctgcgcgc gttcgagcgc gacaaggccg acctgctcga
     1081 tctcggcgtg ccgatccggt acatcacacc cgacgaggac gactcgctcg aggacggcgg
     1141 ctacgtgatc gacctcaagc ggttcaagat gcccgaggtc cggctgacgc ccgacgagat
     1201 ctcggcgctg gtgctcgccg cgtcggtcgc gcacgcgatg cccggcggcg cgtaccccaa
     1261 gatcgtcgat ctcgcgctca agaagctcgc gttcgacctg cccgagctgc ccgacacgcc
     1321 gaccgagttc ccgcggcccg cgccggtgct ggtccacttc ccggagcgcc ggatcacggc
     1381 gaccggcgcg cgcgccgacc tcgccgagat ctacgcgacg atcgaggccg cgacgcgcca
     1441 ccgcaagcgg gtgacgctga cctaccaggc ggcggcgacc ggcatgaggt cgcggcgcga
     1501 cgtcgatccg tacgcgctga tctaccgcga gagcgcctgg ctcgtcgtcg gctggtgcca
     1561 cctgcgccgc gaggtccgga cgttccggat cgaccggatc cacgaggcgg tgatggcgcc
     1621 gcggccgaaa tctcccgact tcgagcgtcc ggccgacttc gacgtcaagg cctacgcgca
     1681 gcgctcggcg tggacgttca acaccgagcc acccgaggag atccagctcg cgctcgcgcc
     1741 cgaggccgcc gaggtcggca acgaggactt cgggctgacc gcggtcaagc ggatcgacgg
     1801 cgaccgcacg ctggtgacgt tcgactgcag caacctcgag ttcgccgcga cccggatcct
     1861 cgccgccaag ggcgcgatcc ggctgctccg cggcaaccgg ctgcgcgccc ggatccacga
     1921 cgagctcgac gcgatcgagg cgcactaccc ggtgctcgac gacgagctcg aggcgctccg
     1981 gcaccatgca cacaacgggc acgacggcgc gaacggcgtg gtcggcctcg gcgcccccga
     2041 ggtggcgggc cccgccgatg ccgcgctgcc cggcggcccg ggagag
//
LOCUS       DASVOR010002083         1858 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_167952,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002083 DASVOR010000000
VERSION     DASVOR010002083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1858
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167952"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            254..658
                     /locus_tag="VF516_38680"
     CDS             254..658
                     /locus_tag="VF516_38680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113741.1"
                     /translation="MSSALGSAVTTARVVRRGEGAHRAPPPRSLRGEDVDLADVGRRG
                     QQLVGAPQQGRRDPALEVRLAAGLIGERVEDAEAARPGAERVPDHGAGLLRGERLGAA
                     QQVRAATSKQKSISQAVLERAAALEWEEMGKL"
     gene            734..1024
                     /locus_tag="VF516_38685"
     CDS             734..1024
                     /locus_tag="VF516_38685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113742.1"
                     /translation="MTPYKGKLDPGVAKAILWDNSKDVEFNVQHSNWRSAKANLTAGA
                     QTMLGKARSIKGGVPTTWEQMDPTAGAPCCTRTTPARAARSRRCAAAAPTRR"
     gene            1172..>1858
                     /locus_tag="VF516_38690"
     CDS             1172..>1858
                     /locus_tag="VF516_38690"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEX8113743.1"
                     /translation="MERAAAAPPTTRPTRRRWTASSSRSAAAASTSATTSSRCRPGSS
                     SAASIRRASQATFLPHPDGLISNNQTTEKYLFGESGKISATPPAKPKAETSPAGSKDA
                     GHAKDEHQDKPGPAQEPAHPQYEAIARNFKSAIPGSTLTWHQALFLPSWGRHVKPSDV
                     TNASMDTVLANIEKQAAALQKVSDHFGKAIHVHCWLRPPAYNKQIGGASNSAHLRGTA
                     TDFHMDGITAE"
BASE COUNT          329 a          679 c          645 g          205 t
ORIGIN      
        1 ccagcgctgc cccgcgagca gcgcgatcgc ggccaccgcg aggatggcgg cgacctccgg
       61 gccgtgccag gcgctcggcg ccagcggcag cgccagcccg gcgctgacca gcccgctcag
      121 cgcgagccag ccctcgcgat gatgctcgcc ctctcgacgc atcgagcaat tgtaagcaca
      181 tcgttacagt tgtctagaac gtcgtgacaa ccttcatcga cggcgcgcgg cgtacgaatc
      241 cttcgtacgt cgcatgtcgt cggcgctcgg atccgccgtg acgacagcgc gcgtggtgcg
      301 gcgcggcgag ggcgcgcacc gggcgccgcc gccgcggtca cttcgcggcg aggatgtcga
      361 cctggcggat gtcgggcggc gaggccagca gctcgtcggc gcgccccagc agggccgccg
      421 cgatccggcc ctcgaggtgc gccttgcggc cggcctcatc ggcgaacgcg tcgaagatgc
      481 cgaagctgcg cggcccggag cggaacgcgt accagaccac ggtgccggcc tcctgcgcgg
      541 cgagcgcctg ggcgctgcgc agcaggtccg cgcggcgacc tcgaagcaga agagcatctc
      601 ccaggcggtg ctggagcggg ccgccgcgct cgaatgggag gagatgggca agctctgatc
      661 ttcgcctcgg ccgcgaccgt atgacgctcc tcacggaggg gccttgcgag gcgtgtctcc
      721 gcgcgctgct accatgacgc cgtacaaggg caagctcgac ccgggggtgg ccaaggcgat
      781 cctctgggac aactcgaagg acgtcgagtt caacgtccag cacagcaact ggcgcagcgc
      841 caaggccaac ctgaccgccg gcgcccagac catgctcggc aaggcgcgct cgatcaaggg
      901 cggcgtgccg accacgtggg agcagatgga ccccaccgct ggcgcgccgt gctgtacgcg
      961 tacaacgccg gcgagggcag cgcgatctcg gcgctgcgcc gcggcggccc caacgcggcg
     1021 atgatcagca cgttcaagga cccgtcgggc aagaccgtca gccacgacta caccgccgag
     1081 atcaaggcga agatggacta cgtcgacggc cacgatccgt tcgcggccgg cggcaaggag
     1141 acgccgggcg gcccggcgac cagcggcggc ggtggaacga gcggcggcgg cgccacccac
     1201 gacacgcccc acccggcggc ggtggacggc atccagcagt cggtcggccg cggcggcgtc
     1261 aaccagcgcc acgacgtcat cgcggtgcag acccggctca agcagcgcgg cgtcgatccg
     1321 cagggcatcc caggccacgt tcctgccgca ccccgacggg ctgatctcga acaaccagac
     1381 caccgagaag tacctgttcg gcgagtccgg caagatctcc gcaacgccgc cggccaagcc
     1441 caaggccgag accagcccgg cgggcagcaa ggacgcgggc cacgccaagg acgagcacca
     1501 ggacaagccg ggcccggccc aggagccggc ccacccgcag tacgaggcga tcgccaggaa
     1561 cttcaaatcc gcgatccccg gatcgacgct gacctggcac caggcgctgt tcctgccgtc
     1621 gtgggggcgc cacgtcaagc cgagcgacgt caccaacgcg tcgatggaca ccgtgctggc
     1681 caacatcgag aagcaggccg ccgcgctgca gaaggtcagc gaccacttcg gcaaggcgat
     1741 ccacgttcac tgctggctgc gcccgccggc gtacaacaag cagatcggcg gcgcgagcaa
     1801 ctcggcgcac ctgcgcggca cggcgaccga cttccacatg gacggcatca ccgccgag
//
LOCUS       DASVOR010002084         3439 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_372829,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002084 DASVOR010000000
VERSION     DASVOR010002084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3439
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372829"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VF516_38695"
     CDS             <1..694
                     /locus_tag="VF516_38695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113744.1"
                     /translation="AAGLVPVADLTHLPPALQHSALWALADGGFETRVVHGALSRGKH
                     DLDVTAFDLETLRERRGEWAYLPVEPPFRIAGTVSVVVCEVDRVFPHVLWKRAGRGDD
                     LADDNMIERAGSIAKLARGSLGMPSSYPAELPRSLPPAALGVKLPEHWRVYGDPVVVD
                     ALLAAGFAATLDRAGRRDLVIELLDSLVIVYPAARDVVGADAFADLTTTALVIVDGVL
                     ACSPALSPRGVE"
     gene            880..1287
                     /locus_tag="VF516_38700"
     CDS             880..1287
                     /locus_tag="VF516_38700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113745.1"
                     /translation="MLIVDDYPGTAETAGKLLRGAGHECRSARSGAEALVVADAFEPE
                     VAILDIGLPDIDGYALIDALRRRLAVRPPYVVAMSGRGHALTAAREIGFDECLLKPVD
                     REQLVRVVELARQWQTPLGPHREYGGANSQGDR"
     gene            1321..1545
                     /locus_tag="VF516_38705"
     CDS             1321..1545
                     /locus_tag="VF516_38705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113746.1"
                     /translation="MLPSDRYAIENVLTTLGASAAADVTVAPSRGVRWTLGLEYIASL
                     REDPGKVLAVSTSTALALVAAGATLHHPEH"
     gene            complement(1617..2792)
                     /locus_tag="VF516_38710"
     CDS             complement(1617..2792)
                     /locus_tag="VF516_38710"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX8113747.1"
                     /translation="MHKLSLHLRPAAFALLLATGLIPAAPMTRALADTDQPARHDDRS
                     TCPLVAKPLALGHRGAAGYRPEHTLASYQLAIDLGADFIEPDLVSTRDGHLVARHEND
                     ISGTTNVASHPEFASRKTTKSIDGVAITGWFTEDFTLAELKTLRARERLPALRPQNAT
                     FDGQFEIPTFEEVIALAQREGQRRHRVIGVYPETKHPTYFQSIGLSLEEPLIRTLEAT
                     GLGNKKDAVFIQSFEVHNLQKLRRMTRLPLIQLIDATGAPFDFVAAGDRRTYADLVTP
                     AGLHEVASYADGIGVNKNLIIPRDATGKLLAPTRLIPDAHAEHLLVHAWTFRAENSFL
                     PLDFQIGDPHNPLFAGLRGDFPAELKRFFGLGLDAAFSDQSDVVVAVRTGLPPCDRR"
     gene            3068..>3439
                     /locus_tag="VF516_38715"
     CDS             3068..>3439
                     /locus_tag="VF516_38715"
                     /inference="COORDINATES: protein motif:HMM:NF021082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2383 domain-containing protein"
                     /protein_id="HEX8113748.1"
                     /translation="MATKSSIDKLNEFLRGEMSAVETYQMALDKIEGSSAARDELLVN
                     LKSHRDRVIALQDAIVALGGTPAQSSGPWGTFAKLVEGAAKVLGDKAAIDALEEGEDH
                     GLKDYRKDLDQLDARGRQLVGG"
BASE COUNT          509 a         1113 c         1286 g          531 t
ORIGIN      
        1 cgccgccggc ctggtgccgg tcgccgacct gacccacctg ccgcccgcgc tgcagcacag
       61 cgcgctgtgg gcgctcgccg acggcgggtt cgagacccgg gtcgttcacg gcgcgctgtc
      121 gcgcggcaag cacgacctcg acgtcaccgc gttcgacctc gagaccctgc gcgagcggcg
      181 cggcgagtgg gcgtacctgc cggtcgagcc gccgttccgg atcgccggca cggtcagcgt
      241 cgtggtgtgc gaggtcgatc gcgtgttccc gcacgtgctg tggaagcgcg ccggccgcgg
      301 cgacgacctc gccgatgaca acatgatcga gcgcgccggc tcgatcgcca agctcgcgcg
      361 cggcagcctc ggcatgccga gctcgtaccc cgccgagctg ccgcgctcgc tgccgccggc
      421 cgcgctcggc gtcaagctgc ccgagcactg gcgggtgtac ggcgatccgg tggtggtcga
      481 tgcgctgctc gccgccgggt tcgcggccac gctcgatcgc gccggccgcc gcgacctggt
      541 gatcgagctg ctcgacagcc tcgtgatcgt ctaccccgcg gcgcgcgacg tggtcggcgc
      601 cgatgcgttc gccgacctca ccacgacggc gctggtgatc gtcgacggtg tgctcgcgtg
      661 ctcacccgcg ctgtcgccgc gcggcgtcga gtagccctac ctgcgggcgt gcgcggtccg
      721 gagtgatgcg ctggagcaca ccgcgcgcgt gcgctcggtc cccgatgagg catcgaggtc
      781 ttgggcgttt gccagacgcc ttggcgcatt gcgctacgta taattacgta gactcccccg
      841 gggacgttgc ccagaatcaa cgggaggacg ccaatgcgtg tgctgatcgt cgacgactat
      901 ccgggcactg cggagacagc agggaagctg ctgagaggcg cgggccacga atgccggagc
      961 gcgagatcgg gcgcagaggc gctggtggtc gcggatgcat tcgagcccga ggtcgcgatc
     1021 ctggacatcg gtcttccgga tatcgatggc tacgccctga tcgacgccct gcggcgccgg
     1081 ctggcggttc gaccgcccta cgtcgtcgcg atgagcggcc gggggcacgc gctcacggca
     1141 gcccgggaaa tcgggttcga cgaatgcctg ctcaagcccg tggaccgaga gcaactcgta
     1201 cgcgtcgtcg agctcgcacg gcagtggcag acgccgctcg ggcctcatcg agaatacggc
     1261 ggtgcaaact cccagggcga ccgctaggcc ggtgacccct tcacgccgcg cacgaccttc
     1321 atgttgccct cggaccgata cgccatcgag aatgtcctga cgaccctcgg ggccagcgca
     1381 gcggcggatg tgaccgttgc tccgagccgg ggcgtacgct ggacgcttgg actggaatac
     1441 atcgcgagcc tgcgcgagga tccggggaag gtgctcgccg tctcgaccag tacagcgctg
     1501 gcgctggtcg cggccggcgc cacgctccac cacccggaac actagccgcc ggcagcagac
     1561 acgggtggct gcagggacac acgggcggca agtgccggat cggcgaggcc gatcgatcac
     1621 cgccggtcgc agggcggcag gccggtgcgg acggcgacca cgacgtcgga ttgatccgag
     1681 aacgcggcgt cgaggccgag gccgaagaac cgcttgagct cggcggggaa gtcaccgcgc
     1741 agcccggcga acagcgggtt gtgcgggtcg ccgatctgga agtcgagcgg caggaagctg
     1801 ttctcggcgc ggaacgtcca ggcgtggacg agcaggtgct cggcgtgcgc gtcggggatc
     1861 aggcgggtgg gcgcgagcag cttgccggtg gcgtcgcgcg ggatgatgag gttcttgttg
     1921 acgccgatcc catcggcgta gctcgcgacc tcgtggagcc cggccggcgt gaccaggtct
     1981 gcgtaggtgc gcctgtcacc ggcggcgacg aagtcgaacg gcgcgccggt cgcgtcgatc
     2041 agctggatca gcggcaggcg ggtcatccgg cgcagcttct gcaggttgtg gacctcgaac
     2101 gactggatga acaccgcatc cttcttgttg ccgaggccgg tcgcctcgag cgtccggatc
     2161 aacggctcct cgagcgacag gccgatgctc tggaagtagg tcggatgctt ggtctcgggg
     2221 tagacgccga tcacgcggtg gcggcgctgg ccctcgcgct gcgcgagcgc gatcacctcc
     2281 tcgaaggtcg ggatctcgaa ctggccgtcg aaggtcgcgt tctgcgggcg cagggccggg
     2341 aggcgctcgc gggcgcgcag ggtcttgagc tcggccagcg tgaagtcctc ggtgaaccag
     2401 ccggtgatgg ccacgccgtc gatcgacttg gtggtcttgc gcgacgcgaa ctcggggtgg
     2461 ctcgcgacgt tggtggtccc ggagatgtca ttctcgtggc gcgccaccag gtggccgtcg
     2521 cgggtcgaga ccaggtcggg ctcgatgaag tcggcgccga ggtcgatcgc gagctggtac
     2581 gacgcgagcg tgtgctccgg gcgatagccg gcggcgccgc ggtggcccag ggccaggggc
     2641 tttgcgacga gcggacaggt gctgcggtcg tcgtgcctgg ccggctgatc ggtgtcggcc
     2701 agcgcgcggg tcatcggcgc ggccgggatc aggccggtgg caaggagcag ggcgaaggcg
     2761 gccggacgga gatgcaggga cagcttgtgc atgtacttcc tccttgggcg agtcggttgc
     2821 gggccgtcga atctacgagg cgacgatgac cgtggcgcgg cgcctctgcg gcaaccctgc
     2881 gatgtgagcc cggtcgatcc cggtcgcgag atctgtagtg cagggagctg tcatgccgct
     2941 cacagatgcc ggtgcgagtg ctggtccatg cgtcgagaga gcgctggttt gccgtcgtcc
     3001 gtcctgcgac gtcttccgat cgcacgtcgt ttgcaatgct tcgccttgta acgacggagg
     3061 aatccccatg gcaaccaagt caagtatcga caagctgaac gagttcctgc gcggtgagat
     3121 gtcggccgtc gagacctacc agatggcgct cgacaagatc gaaggctcga gcgctgcgcg
     3181 cgacgagctg ctggtcaacc tcaagtcgca ccgggatcgc gtgatcgcgc tgcaggatgc
     3241 gatcgtcgcg ctcggcggca cgccggccca gagctcgggc ccgtggggca cgttcgccaa
     3301 gctcgtcgag ggcgcggcga aggtgctggg cgacaaggcg gcgatcgatg ccctcgagga
     3361 aggcgaggac cacggcctga aggactaccg gaaggacctc gaccagctcg acgcccgggg
     3421 ccggcagctc gtcggaggg
//
LOCUS       DASVOR010002085         3182 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_167973,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002085 DASVOR010000000
VERSION     DASVOR010002085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167973"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..747)
                     /locus_tag="VF516_38720"
     CDS             complement(175..747)
                     /locus_tag="VF516_38720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8113749.1"
                     /translation="MTAAELGALARRAQAGDGTARHELLVELYGAVRKHVYLILGGGA
                     IADDAVQEAMIALHRGLASFRGDTASPRTWALAIATRIARRLRRRESRYQLVDDGTVD
                     VGVFDLAPAAAAELAVLRRALATLSPKKRDAFVLMAIFELSAEEAGRALGTFANTAAS
                     RFRHARAELEARFTKLDESVAARATDRREP"
     gene            829..1881
                     /locus_tag="VF516_38725"
     CDS             829..1881
                     /locus_tag="VF516_38725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113750.1"
                     /translation="MRQFCIARMARLVMLVMLGIPGMAAAQAPGQVEPASAPFLEKRV
                     PEELAIEGIVLSRRNLALQIEQLGDRWLVSLVDLTTGRVAASTKVDALPADREAAVAA
                     MTHVVSDLAAQIVGRSEPPPPPPPPVAAPPPPPPPAVIDDRAEREQRELAERKFRRQA
                     IRIGDTLLVTTTGSVVSVSQRWVVRQGDLNLELEPEEFYAKVGRPDLGEAYASRRKLM
                     LGSYIAAGVVLVAGTALAFGLNQHKDCPTNLQDPTYFMCADSNLNADQNKIITSTAAL
                     GVSTVGLFVGLWYQLHLHPISENEAKSLADVYNQRLRRELGLPVVVREPLLRDVKLTP
                     FVARSDTGGLALSARF"
     gene            complement(1965..3014)
                     /locus_tag="VF516_38730"
     CDS             complement(1965..3014)
                     /locus_tag="VF516_38730"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEX8113751.1"
                     /translation="MLDTKPVFAILSIDGGGIRGIIPASVLVAFEKYFEMPVSQLFDL
                     IVGTSTGGIISLALTCSKDGTSPAFTAQQVLDLYNNQDNDAQIFDPTLPWIHPFSPRG
                     TPPPIENALLNAKYKASGVQGFLQAQFGSATLGKAIAPVACVSYRMDGTPQPWFFRSW
                     AATETGGDFALVDVGRATGAAPIYFPPVTISSTDSQLTGTFADGGVCANDPALVALSQ
                     AASLLQSRGDSLWNYQVWLVSIGTGEPALTVQPSDGGVYGWFNSGNLLSVLMDGPMQI
                     TDGAAGMLLDGGGYTYARIQPPLTGPGYSCSPALDDYTTDNLANLLRAGQAAVGSDAF
                     ARAREALAGRRAARR"
BASE COUNT          479 a         1106 c         1128 g          469 t
ORIGIN      
        1 cgacgagcac ggccgcagca gccgcaacgc cggcggccag ccacggcacc cagcgccgcg
       61 gctgcggtgc gggcgcggac cgcgcccagc gatcgagatc gcggacgtat cgctcggcct
      121 cgccgcggtc gacggtcgcg ctccgggcgc gctcgatcag agcgtcgagg tcggtcatgg
      181 ctccctccga tctgtggcgc gagccgccac ggactcgtca agttttgtga atcgggcctc
      241 gagctcggcg cgggcgtgcc ggaaccggct cgccgcggtg ttggcaaacg tccccagcgc
      301 gcgcccggcc tcctccgccg agagctcgaa aatcgccatc aagacaaagg catctcgctt
      361 cttggggctg agggtcgcga gcgcccggcg cagcaccgcg agctcggcgg cagccgccgg
      421 cgccagatcg aacacaccta catctacggt gccgtcgtcg accagctgat agcgcgactc
      481 gcggcggcgc agccggcggg cgatccgcgt cgcgatcgcc agcgcccagg tccgggggct
      541 cgcggtgtcg ccgcgaaagc tcgcgaggcc gcggtggagc gcgatcatcg cctcctggac
      601 ggcgtcatcg gcgatcgccc ccccgccgag gatcaggtag acgtgcttgc gaaccgcgcc
      661 gtagagctcg accagcagct cgtggcgagc cgtgccgtcg ccggcttgtg cccgccgagc
      721 cagtgccccc agctcggccg cggtcatgcg ctccggtctg ccgcaggctg cccgctcgca
      781 gccagaaatc ttttgacgaa ccggaccggg ttcgagacac aacggggcat gaggcagttc
      841 tgtatcgcac ggatggctcg gctggtcatg ctggtcatgc tggggatccc ggggatggcg
      901 gcggcccagg ctccggggca ggtcgagccg gcgagcgccc cgttcctcga gaagcgcgtc
      961 cccgaggagc tcgcgatcga ggggatcgtc ctgtcgcgac gcaacctcgc acttcagatc
     1021 gagcagctcg gcgacaggtg gctggtctcg ctggtcgatc tcaccaccgg gcgcgtggcg
     1081 gcgtcgacca aggtcgatgc gctgcccgcc gatcgcgagg ccgcggtcgc cgcgatgacc
     1141 cacgtggtgt ccgacctcgc cgcccagatc gtcgggcgat ccgagccgcc gccaccaccg
     1201 ccgccgccgg tcgcagcccc cccgccgccg ccaccgccag ctgtgatcga cgatcgcgcc
     1261 gagcgcgagc agcgcgagct cgccgagcgc aagttccggc gccaggcgat ccggatcggc
     1321 gatacccttc tggtgaccac gaccggtagc gtggtgagcg tgagccagcg gtgggtcgtc
     1381 cgtcagggag atctcaatct ggagctcgag ccggaggagt tctatgccaa ggtcggccgt
     1441 cccgacctcg gcgaggccta cgcgagccgc cgcaagctca tgctcggaag ctacatcgcg
     1501 gccggcgttg tgctggtggc cggaacagcg ctcgctttcg gcttgaacca gcacaaggac
     1561 tgcccgacta acctgcaaga ccccacgtac ttcatgtgcg ccgacagcaa cctcaacgcc
     1621 gaccagaaca aaatcatcac gtcgaccgcg gccctggggg tgagtacggt cgggctcttc
     1681 gtcggactgt ggtatcagct tcacctgcat ccgatcagcg agaacgaagc caagtcgctc
     1741 gccgacgtct acaaccagcg gctgcggcgc gagctcgggc tgccggtcgt ggtccgcgag
     1801 ccgctgctgc gcgatgtgaa gctgacgccg ttcgtcgccc gcagcgacac cggagggctg
     1861 gcgctgagcg cgcggttctg atcgccttgc aacgccgcga gctcacgccg cgttgctgag
     1921 gatcgactgt tcgaggtaca ccacagctcg gcgagctgtc gcgctcagcg tcgtgcggcc
     1981 cggcgcccgg cgagcgcctc gcgcgccctg gcgaaggcgt cggatccgac ggcggcctga
     2041 ccggcgcgca gcaggttggc gaggttgtcc gtggtgtagt cgtcgagcgc ggggctgcag
     2101 ctgtagcccg ggcccgtgag cgggggctgg atgcgggcat aggtgtagcc gccgccgtcg
     2161 agcagcatgc cggcggcgcc gtcggtgatc tgcatcggtc cgtccatcag cacgctgagg
     2221 aggttgccgc tgttgaacca gccatagacg ccgccatcgc tgggctggac ggtgagcgcg
     2281 ggctcgccgg tgccgatcga gaccagccat acctggtagt tccacaggct gtcgccgcgc
     2341 gactggagca ggctcgccgc ctgcgacagc gccaccagcg ccgggtcgtt cgcgcacacg
     2401 ccgccgtcgg cgaacgtgcc cgtgagctgg ctgtcggtgc tggagatcgt caccggcggg
     2461 aagtagatgg gcgcggcgcc ggtggcgcgg cccacgtcga ccagcgcgaa gtcgccgccg
     2521 gtctcggtgg cggcccagct gcggaagaac cagggctgcg gcgtcccgtc catgcgatac
     2581 gagacgcacg cgaccggcgc gatcgccttg ccgagcgtcg ccgagccgaa ctgtgcttgc
     2641 aggaatccct ggacgcccga cgccttgtac ttcgcgttca gcagcgcgtt ctcgatcggc
     2701 ggcggcgtcc cgcgcggcga gaacgggtgg atccagggga gcgtcggatc gaagatctgc
     2761 gcgtcgttgt cctggttgtt gtagaggtcg agcacctgct gcgccgtgaa cgccgggctc
     2821 gtgccgtcct tggagcaggt cagcgcgagc gagatgatgc cgcccgtcga ggtgccgacg
     2881 atcaggtcga acagctgcga gacgggcatc tcgaagtact tctcgaacgc caccagcacc
     2941 gaggccggga tgatcccgcg gatgccgcca ccatcgatgc tgaggatcgc aaagaccggt
     3001 ttcgtgtcga gcatgttcac ctccggccat gatgtgtagg tcgcccctcg cagcgacgcc
     3061 attacggcgg attacaggct cgccgaggtc gccgagttgc gccggctaga acgcgccgcc
     3121 gacgccgaac gtgaagccgc tgccgcggct cggcgcgacc atcgggcccc agctgcgggc
     3181 cg
//
LOCUS       DASVOR010002086         4748 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_256468,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002086 DASVOR010000000
VERSION     DASVOR010002086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4748
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256468"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..778)
                     /locus_tag="VF516_38735"
     CDS             complement(170..778)
                     /locus_tag="VF516_38735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113752.1"
                     /translation="MTHGNASSNLDPLAARLASSPVAEMATGLGDDPHVNELWKEFPD
                     PNLYASLVVDGRRAEPVRFGAAVVVFAKQRERLLQIEPHAVADVFASALQHNLAGYAY
                     PWGHLWADPDPVGYLGQVFVQIGRPALPALEALLDDATPRDTYLGSEEATEMAQRRYR
                     VKDFAAVYIAQILQLELPWEPDLARRDAAIAKLRQSIPRSGR"
     gene            complement(873..1124)
                     /locus_tag="VF516_38740"
     CDS             complement(873..1124)
                     /locus_tag="VF516_38740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113753.1"
                     /translation="MKDTGDGGATSKLPIPTVLTNPAVAPLYSDPQMKTEVKDLPVGS
                     RMESRGARTAHGSYSVKLVDGPDTGKTGYVDHTLIQQER"
     gene            complement(1133..2290)
                     /locus_tag="VF516_38745"
     CDS             complement(1133..2290)
                     /locus_tag="VF516_38745"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8113754.1"
                     /translation="MALPVQRKPGPDAAASEPVSAPSSGGHALPDDLQAKMGRSFGAD
                     FSSVRVHEGDHATALGALAYTQGTDIHFAPGQYQPTSQSGQQLVGHELAHVVQQSQGR
                     VAATTQAKGVDMNDDSSLEHEADEAGARAARGEPAGMSGGSPSSAIAGAMQRKVIQRN
                     AEVPTHYGKFKTTKFEKVGTTGVSCVIEFHPDENVIDAKKIGLSQTAKITYDNGAHTG
                     IDPTKEGRRVKSGGGQDDVLDRISSQNNPIYGAAPLGARDGLDKTTADNNATGNPTKV
                     GPASDGGNATYQLGFAFTEAGVKKKKEAALFDRPQGSANMFETTALGLEGRDAGQYFG
                     SVKWGYEKKPGDVEPKDIELVSMGVPSQNYLAARSRRSMPAGPGPASSTTS"
     gene            complement(2451..4541)
                     /locus_tag="VF516_38750"
     CDS             complement(2451..4541)
                     /locus_tag="VF516_38750"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8113755.1"
                     /translation="MLLIALVACGGSKPAPQAPPSPPANPVAEAPTPAPAAPAAPAPE
                     AKPAGHPKDDLISRQVIFGNPERIGPQISPDGKQLSWLAPKDGVLNVWVAPIGKLDQA
                     RAVTSDTKRPIRQYAWAFTSKHILYLQDTGGDENFHLYRVDIADGKATDLTPYEGARA
                     QILGASPRQPTTLMVGTNDRDKKVFDVYKVDLLTGKRTLAAQNNDGFAGFELDHDLAV
                     RIAAKKLPDGSTQLLTPETKAADTKWKLFETIPFEDADTTDVLDFAPGNKAVYMRDSR
                     GRDTAALVQVDLATKKSTVLAEDTKADAGEVLAHPTKHNLQAVGFNYLTESWKVLDPS
                     IQKDLDALAKLDGGEVHVTSRTTDDKLWIVTTTSPQHPGHYYLWDRGKQKGTFLFAAR
                     PELEKQPLVKMTPVEIKARDGLTLVSYLSLPAAADPDGDGKPNSAVPMVLFVHGGPWA
                     RDSWGYNPIHQMLANRGYAVLSVNYRGSTGFGKKFLNAGNLQWGKKMHDDLLDAVAWA
                     AQQGVTSKDSVCIMGGSYGGYATLAGLTLTPDVFRCGVDLVGPSNLMTLLATIPPYWL
                     PAVATFHTRMGNPETAEGKALLIAASPLTHAADIRRPLLIGQGANDPRVKQAESEQIV
                     GAMKSHHLPVSYIVFPDEGHGFARPENNIAFFGAAEAFLSAHLGGSYLPLTKAEVGAS
                     SMQVKDGRDGVPGL"
BASE COUNT          615 a         1598 c         1698 g          837 t
ORIGIN      
        1 gcccgaccgc acgggcctgg ccaacctgct ccgccgcgcg tcgcacctgg tcgtcgacct
       61 cggcgaccgg ttcgcccggc tcgacctgcc gcgcgtcgtg atcggcggcc gcggcgcccg
      121 caccctggtc gtcgacgccg ccgccgagct gcgctgagcg cgcccgatgt cagcggcccg
      181 atcgcgggat tgactgacgg agcttggcaa tcgccgcgtc gcgcctggcg agatcgggct
      241 cccacggcag ctcgagctgg aggatctggg cgatgtagac ggcggcgaag tccttgacgc
      301 ggtatctgcg ctgggccatc tcggtagctt cctcgctgcc caggtacgta tcgcgtgggg
      361 tcgcatcgtc gagcagcgcc tccagtgctg gaagcgccgg ccgcccgatc tggacgaaca
      421 cctggccgag ataaccaacc ggatccggat cggcccagag atggccccac gggtacgcgt
      481 agccggcgag gttatgctgc agcgcgctgg cgaacacgtc cgcgacggcg tgcggctcga
      541 tctggagcag ccgctcgcgt tgcttcgcga acaccaccac cgccgccccg aagcgaaccg
      601 gctccgcgcg gcgtccgtcg acgaccagcg aggcgtagag gttcgggtcc gggaactcct
      661 tccagagctc gttgacgtgc ggatcgtcgc ccagcccggt cgccatctcg gccaccgggc
      721 tcgatgccag ccgggcggcc agcgggtcca ggttcgagga tgcattgccg tgtgtcatgg
      781 aggagcaacc tgcccaaagg acgccgagga tcacagcatg gagcagaagg cgcatcgtgg
      841 cgaccagcgc accgagaggg tgcatcgcga tcttagcgct cctgctggat gagcgtgtgg
      901 tcgacatagc cggtcttgcc ggtgtcgggg ccgtcgacga gcttcacgct gtacgagccg
      961 tgggcggtgc gggcaccccg cgactccatg cgactgccga ccgggagatc cttcacctcg
     1021 gtcttcatct gcggatcgct gtacagcggt gcaaccgccg ggttggtgag caccgtcggg
     1081 atgggcagct tggacgtagc gccgccatcg ccagtatcct tcatgtcgag cgtcaggatg
     1141 tagtactgct tgccggtccc ggtcccgctg gcatcgagcg tctcgacctc gcggcgagat
     1201 agttctggct cggcaccccc atcgagacca gctcgatgtc cttcggctcg acatcgccgg
     1261 gcttcttctc gtagccccac ttcacgctgc cgaagtactg gccggcgtct cggccctcga
     1321 gtccgagggc ggtggtctcg aacatgttgg cgctgccctg tggacggtcg aacagcgccg
     1381 cttccttctt cttcttgact ccggcctcgg tgaacgcgaa cccgagctgg taggtggcat
     1441 ttccgccgtc ggacgccggg cccaccttgg tgggattccc cgtcgcgttg ttgtccgcgg
     1501 tggtcttgtc gaggccgtcg cgggcgccga gcggggcggc gccgtagatc ggattgttct
     1561 ggctggagat gcgatcgagg acgtcgtcct ggccgccgcc tgacttcacg cgtcggcctt
     1621 ccttggtggg atcgatcccg gtgtgggcgc cgttgtcgta ggtgatcttg gcggtctgtg
     1681 acaggccgat cttcttggcg tcgatgacgt tctcgtcggg gtggaactcg atgacgcagc
     1741 tgacgccagt ggtaccgacc ttctcgaact tggtggtctt gaacttgccg tagtgggtcg
     1801 ggacctcggc gttgcgctgg atcaccttgc gctgcatcgc cccggcgatg gcactgctgg
     1861 gcgagccgcc cgacatgccg gccggctcgc cgcgcgcggc gcgcgcaccg gcctcgtctg
     1921 cctcgtgctc gagcgacgag tcgtcgttca tatcgacgcc cttggcctgc gtggtcgcgg
     1981 ccacgcggcc ttgcgactgc tgcaccacgt gcgcgagttc atggccgacg agctgctgcc
     2041 cgctctggct cgtgggctgg tactggccag gcgcgaagtg gatgtcggtg ccctgggtgt
     2101 aggcgagcgc acccagggcg gtggcgtggt cgccttcgtg gacccggacc gacgagaaat
     2161 cggcgccgaa cgagcggccc atcttggctt gcaggtcatc gggcagggcg tgaccgccgc
     2221 tggacggtgc cgagaccggc tcgctggccg ctgcatcggg gcccggcttc cgctggaccg
     2281 gaagcgccat cgtcagcgtg cacttgccgg gggcgagcgc ctggtcgccg gagcctcgcc
     2341 ccggttcccg acccgcccgt gctctctccc ctgcctgccg taactcatgt gccttctacg
     2401 gtaggcgacc tcacaacctc cccggctgga atggccggtg gctcacccgg ctacaggccc
     2461 ggcacgccgt cgcggccgtc cttgacctgc atcgacgacg ccccgacctc ggccttggtc
     2521 agcggcaggt agctgccgcc gaggtgcgcc gacaggaacg cctcggccgc gccgaagaac
     2581 gcgatgttgt tctcgggccg ggcgaacccg tggccctcgt cggggaacac gatgtagctc
     2641 accggcaggt ggtgcgactt catcgccccg acgatctgct cggactcggc ctgcttgacc
     2701 cgcgggtcgt tggcgccctg gccgatcagc agcggccgcc ggatgtccgc ggcgtgggtc
     2761 agcggcgagg ccgcgatcag cagcgccttg ccctcggcgg tctccgggtt gcccatccgg
     2821 gtgtggaacg tcgcgaccgc cggcagccag tacggcggga tcgtggcgag cagcgtcatc
     2881 aggttcgacg gcccgacgag gtcgacgccg cagcggaaca cgtccggggt cagcgtgagg
     2941 ccggcgagcg tggcgtagcc gccgtagctg ccgcccatga tgcacacgct gtccttggag
     3001 gtgacgccct gctgggccgc ccacgcgacg gcgtcgagca ggtcgtcgtg catcttcttg
     3061 ccccactgta gattcccggc gttgaggaac ttcttgccga acccggtcga gccccggtag
     3121 ttgaccgaca gcacggcgta gccgcggttg gccagcatct ggtggatcgg gttgtagccc
     3181 cagctgtcgc gcgcccacgg gccgccgtgg acgaacagga ccatgggcac cgcgctgttc
     3241 ggcttgccgt cgccgtcggg atccgccgcg gccggcagcg acaggtagct gaccagggtc
     3301 aggccgtcgc gcgccttgat ctcgaccggc gtcatcttga ccagcggctg cttctcgagc
     3361 tcgggccgcg ccgcgaacag gaacgtgccc ttctgcttgc cgcgatccca caggtagtag
     3421 tgcccggggt gctgcggcga ggtcgtggtg acgatccaga gcttgtcatc ggtggtgcgc
     3481 gacgtgacgt ggacctcgcc accgtcgagc ttggccaggg cgtcgaggtc cttctggatc
     3541 gacggatcga gcaccttcca cgactcggtc aggtagttga agccgaccgc ctgcaggttg
     3601 tgcttggtcg ggtgggccag gacctcgccc gcgtcggcct tggtgtcctc ggcgagcacc
     3661 gtcgacttct tggtcgcgag atcgacctgg accagcgccg cggtgtcgcg gccgcggctg
     3721 tcgcgcatgt acacggcctt gttgcccggc gcgaagtcga gcacgtcggt ggtgtcggcg
     3781 tcctcgaacg ggatcgtttc gaacagcttc cacttggtgt cggccgcctt ggtctcgggc
     3841 gtgagcagct gggtcgagcc atcgggcagc ttcttcgcgg cgatccgcac ggcgaggtcg
     3901 tggtcgagct cgaacccggc gaagccgtcg ttgttctgcg cggcgagcgt gcgcttgccg
     3961 gtcaagaggt cgaccttgta gacgtcgaac accttcttgt cgcgatcgtt ggtgccgacc
     4021 atcagcgtgg tcggctggcg cgggctggcg ccgaggatct gggcgcgggc gccctcgtac
     4081 ggcgtcaggt cggtggcctt gccgtcggcg atgtcgaccc ggtacaggtg gaagttctcg
     4141 tcgccgccgg tgtcctgcag atagaggatg tgcttgctgg tgaacgccca cgcgtactgc
     4201 cggatcggcc gcttggtgtc cgaggtcacc gccctggcct gatcgagctt gccgatcggt
     4261 gcgacccaca cgttgagcac gccgtccttg ggcgcgagcc aggacagctg cttgccgtcg
     4321 gggctgatct gcgggccgat gcgctcgggg ttgccgaaga tgacctggcg cgagatcagg
     4381 tcgtccttgg ggtggcccgc cggtttggcc tccggagcgg gcgcggcggg cgcggccggg
     4441 gccggggtcg gggcctcggc gacggggttg gccggcgggg acggcggtgc ctggggagcc
     4501 ggcttgctac ctccacaggc gaccagagcg atcagcagca atctcttcct gcccgtcggc
     4561 gtaccacatg gccggcgctc cgggcagaac cggctgccgt caaccggcgc gtccgccgat
     4621 cggcgcgtct accgggccgc gctaccgagc gagctccagc tcgagcccgc gcgccacggc
     4681 gatcagcgcg tccgcggtgt cgagctccgg atcggtgagc acgcggtcga gcagcgcggc
     4741 gagcagcc
//
LOCUS       DASVOR010002087         1904 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_51857,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002087 DASVOR010000000
VERSION     DASVOR010002087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1904
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51857"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="VF516_38755"
     CDS             complement(<1..315)
                     /locus_tag="VF516_38755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113756.1"
                     /translation="MSTARKTLLMVALAALAVGGCGGARSDSPEPLEAARNIQSHDTR
                     SGAPGKGHMGEPAGAPDEMAAMPPELQTCHAVLAPRWHAEHGPERIADTCGAIGELRA
                     GAD"
     gene            361..840
                     /locus_tag="VF516_38760"
     CDS             361..840
                     /locus_tag="VF516_38760"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEX8113757.1"
                     /translation="MQLFVIRHAVAEDVGPGIDDTARELTEAGKTKLRRAVRGLRALD
                     IRFERILTSPWKRAVQTARQLEPIAASPPVTTDLLTQPPRAELLALIAEGGDTTAVVG
                     HEPWLGELAAWLAFGDTRHGDALIIKKCGVLWLEGTAVPGGMQVRAAIPPRLLRAIR"
     gene            1263..1511
                     /locus_tag="VF516_38765"
     CDS             1263..1511
                     /locus_tag="VF516_38765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113758.1"
                     /translation="MHLGAPMKAARIFSMPVIDRARRCRSASGSPAMRRWQNHRSTLR
                     ADPQRHRVNSAVRAPSQLVSRLQTPSLDCLNFPRPLER"
     gene            1537..>1904
                     /locus_tag="VF516_38770"
     CDS             1537..>1904
                     /locus_tag="VF516_38770"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8113759.1"
                     /translation="MQLVPHSAAVMRTKELAQSLDLDLLLRTYTFIPRKILRDTVREF
                     GSQLEGKVLDVGCGSQQYRKFLNYEQYFGIEWSVDKRPPVVADVTQIPFCDAAFDSAL
                     CTEVLEHLPEPGRCLDEIHRV"
BASE COUNT          273 a          703 c          631 g          297 t
ORIGIN      
        1 atccgcgccc gcgcggagct cgccgatcgc cccgcaggtg tcggcgatcc gctcggggcc
       61 gtgctcggcg tgccagcgcg gcgccagcac cgcgtgacac gtctgcagct cgggcggcat
      121 cgccgccatc tcgtccggcg caccggcggg ctcgcccatg tggcccttgc cgggggcccc
      181 gctgcgggtg tcgtggctct ggatgttgcg cgccgcctcg agcggctccg gcgagtccga
      241 cctcgcgccg ccgcagccgc ccacggccag ggcggccagg gcgaccatca gcagcgtctt
      301 ccgcgcggtg ctcatctccg gcgctttcgc acgcgctcgg ccgcgtgtca aataccgcac
      361 atgcagctgt tcgtgatccg ccatgccgtc gccgaggacg tcggcccggg catcgacgac
      421 accgcccgcg agctgaccga ggccggcaag accaagcttc gccgggccgt ccgcgggctg
      481 cgcgcgctcg acatccggtt cgagcggatc ctgaccagtc cgtggaagcg cgccgtccag
      541 accgcgcgcc agctcgagcc gatcgcggcg agcccgccgg tcacgaccga cctgctcacc
      601 cagccgccgc gcgccgagct gctcgcgctg atcgccgagg gcggtgacac caccgccgtg
      661 gtcggccacg agccgtggct cggcgagctc gccgcgtggc tggcgttcgg cgacacccgg
      721 cacggcgacg cgctgatcat caagaagtgc ggcgtgctgt ggctcgaggg caccgccgtc
      781 cccggcggca tgcaggtgcg cgccgccatc ccgccccgcc tgctgcgcgc gatccgctga
      841 gctgtggctg aatctcgttc tagccgggcg atgtagccgg cttgaatttt tccgtgacac
      901 tgttcaccgc accgcggtaa cgtggtcgat tttgccggag taactgtacc caaaggaacg
      961 tcattggtcc tatggtcgta ccaggcgtgc gcgacccgtg ccgcctcggc gacacggctg
     1021 tcgcacgaca aggagggtgg tgttgttgcg agctcgaacg ccgtcgatcg ctccccatgc
     1081 gaatcactcg ccgatgcgag ctcggtcaca gccacggtcg ctgcgctcgc tcccgcagat
     1141 cacctccgag cggcggacgt tgatcgatct cgaagccgcg aggcacgtcc ctgccgcgcg
     1201 ttgccaggtg tccacacctg ccggtgcacg ggcgatgcgg atggccgccg ttgcatggcg
     1261 gcttgcatct cggcgcgccg atgaaagcag cgcggatctt ttccatgccg gtgatcgacc
     1321 gcgcacggcg atgccgctcc gcatccggtt cgcccgcgat gcgccgatgg cagaaccacc
     1381 ggagcacgct gcgcgccgac ccgcagcgcc accgcgtcaa ttcggcggtg cgcgcgccgt
     1441 cacagctggt cagtcgattg cagactccgt cgctcgattg tttgaacttc ccgcggcccc
     1501 tcgaacgttg aacctgcaag aacgcgaggc aacaccatgc agctcgtccc tcacagcgcc
     1561 gccgtgatgc gaaccaagga actggcccag agcctggacc tcgatctgct gctgcgcacg
     1621 tacacgttca tcccgcgcaa gatcctgcgg gacaccgtgc gcgagttcgg cagccagctc
     1681 gagggcaagg tgctcgacgt cggctgcggc tcgcagcagt accgcaagtt cctcaactac
     1741 gagcagtact tcgggatcga gtggagcgtc gacaagcgcc cgccggtggt cgccgacgtc
     1801 acgcagatcc cgttctgcga cgccgcgttc gactccgcgc tgtgcaccga ggtgctcgag
     1861 cacctgccgg agcccgggcg ctgcctcgac gagatccacc gcgt
//
LOCUS       DASVOR010002088        10054 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_460266,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002088 DASVOR010000000
VERSION     DASVOR010002088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10054
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460266"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VF516_38775"
     CDS             <1..271
                     /locus_tag="VF516_38775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113760.1"
                     /translation="FSPIWGAPHLRRETAAHITDAMRTLLVRAAGYEVKAMEFVPAEH
                     TRKNTLIRAVKRSAGDADALAAYEALRTATGGAGIALAARLAASG"
     gene            897..1313
                     /locus_tag="VF516_38780"
     CDS             897..1313
                     /locus_tag="VF516_38780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113761.1"
                     /translation="MHPPELDRPRESDRFDPVVEGARLGLTRELSLVIWQRICADIRD
                     ARGRLDEDQARQQFRELAARVAARGGRLRPDVGRLTRVEIEDRADSLDTWCGDALTAH
                     TPRARHARRGGGAAPGDAGRARAVERRVATGKRAGA"
     gene            1378..2091
                     /locus_tag="VF516_38785"
     CDS             1378..2091
                     /locus_tag="VF516_38785"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8113762.1"
                     /translation="MERAFGTTFADVVIRTDERAAARAASLDVLAYADGREIGFSPGW
                     WSPNTPEGQRTLVHELAQLERPAAELIAHAPTVAAASLGSSRGWELWDPRAAHRDGWR
                     LPPGDRRKDARPLESRRIAPRRGALDGLVVLGSWERTEHVRDAEARLRERAALLDGVA
                     ADSPLARLGRDGGEPLADELRARMEQLFGHGFAHVRVHTDLAAAQATSALGARAFAVG
                     HHVYFGAGLFQPGTSMAIA"
     gene            2242..4677
                     /locus_tag="VF516_38790"
     CDS             2242..4677
                     /locus_tag="VF516_38790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113763.1"
                     /translation="MRPIAKRIVERATFVQGELVAALMLAYSDVDPAGRQLTTAQRDL
                     GEFADFVIDQTLGPDGVERLIAQVVPLREQIITARGQTGRAGITRPADLMAGFGEPKH
                     IGGLEKPLRKVLDGLRRSHAAGSDVGEKLRHLIEDIRSVHLIEKIIVITAAAAFTAGL
                     AGSAAAGLAGEVGLGGLAGAASFGAEVVAFTTVSRMAEGAMIGPSENSFGHDLAINAL
                     TFGVLKSVGRVFAKIVKLGADPSMLAKAAYRAGSFATVAVSLQLFAEAQHVFEHGSAM
                     SNEQRASSIAQNAIVMAALELGHFIVKAPAARGLGSIDAALGKQLAALGGERAAIASQ
                     LEALRAQKLTPQQVNELLAKIQDLYGRELQLFDAAAKRGATQPALKAAIAVYQQQIAA
                     VELKLARAGVANAHTGPQLFQQVRPGVVGFQPAARPIIDRFYQEQGGRVVAGQRPGEL
                     IGTLPDGETTHFLSDELVKDEQRAREQERKVEARRKQLLDEIGSAVGPEALPRFTDLP
                     TETLERLARRPAPELAKLARLAPEELARLLALPELQLGRLLGLPAEQLTALARLAPDQ
                     LAKLGGLEGQSLVNLARRTPVELQRILALDPADVKILAELKFGHATDLLGVSPSPAHL
                     RAAARVCSRFMAAGMSEKAAVDRTVEIGTSGDRLLLMGDAFQGGGRGTSKQVVHADGI
                     EAHEVAQAIDFIRLKGGGGQFEFGAVGLEGIEGHYVPEGAKQRIPVSMKVFTTADAVN
                     VLQRADLNAGHVDAGIGAELFVYTNADAKAIYDLAKTPPPGGAAAQVFKRMTFVCRDG
                     TLIYDSGRWQPPR"
     gene            4683..5051
                     /locus_tag="VF516_38795"
     CDS             4683..5051
                     /locus_tag="VF516_38795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113764.1"
                     /translation="MDTIIYILPSLHVPTSWGMLEDSIRRWSGADASRLWGPTINLSA
                     WREAAHRPELPRDLILDATYRYRIDLAGDDHGPWINYHPHHLLTHPRNLFGEMGPTLG
                     PSEIDHIIKGIQQTGYFSRS"
     gene            5927..6934
                     /locus_tag="VF516_38800"
     CDS             5927..6934
                     /locus_tag="VF516_38800"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEX8113765.1"
                     /translation="MQTDLTPAQLEDRARAAFAAARAQLYVECEKLHRKLWPNDVLPA
                     GVTDPAAQHKVIQRVVDELGKDHPRPDELVAAHGRNLDALRAFIQNHDLVALPPRDTL
                     RVEPMPAFKRGSTAAEYLAPGVLVRNPNWRATYYVDPIDPSWPPDRVESYLRGQNDYV
                     VQLVAAHEAYPGHHTQYFHSKRNLNPLRAVLWNGPMVEGWAVYGEGLMVGLGWGGERN
                     DRFRVFELVEILVSTANTIIDIELQSGRMTDEAAVRFMVEDGFQPRAQAEKKLVRAKL
                     DSTQLCQYYLGLDEIRSLERDYKAQLGARFKQRTFDEALIGHGSLAVKFLREYLLGAG
                     R"
     gene            7081..8805
                     /gene="kaiC"
                     /locus_tag="VF516_38805"
     CDS             7081..8805
                     /gene="kaiC"
                     /locus_tag="VF516_38805"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock protein KaiC"
                     /protein_id="HEX8113766.1"
                     /translation="MPTRRRRAPSAGLAKSPSGIDGFDRITQGGLPSGRTSLVCGGAG
                     CGKTLFAIEFLVKGALLYDEPGVFVAFEETQEELIRNVASLGIRLDELIARKLIAFDF
                     IRVERSEIEEAGEYDLEGLFVRLAHAVSSIKARRIAIDSLEALFSTFANEALLRAELR
                     RLFRWLKDKKLTAVITGEKGEGALTRHGLEEYVSDCVVALDHRVVDQSSTRRMRIVKY
                     RGSAHGTDEYPFLIDEKGFSVLPVTSLDLDYEVSTERISTGIASLDGMLEGGGYFRGS
                     SVMVSGSAGTGKTSLAALFAAASCKRRERTLYFAMEESASQIQRNMASIGFDLAELRR
                     KKMLDLRAARPTVYGLEMHLVAMHNAIEEFEPRAVIIDPISSLSATGTQREVKAMLVR
                     LFDYLKNKQITCLVTSLAAAQVGEQTELEMSSLIDTWIQVRDLELAGERTRGLFLIKA
                     RGMGHSNQVREFVIGSHGIDLIPVEIGPSGVLTGSARENRENERRTEAMAREHERERK
                     LRQLERRRTAVEAQIAAMRAELEAEASELEAAASEERERTRRTSEDEARRGATRSAGR
                     ATTNGERR"
     gene            8802..9146
                     /locus_tag="VF516_38810"
     CDS             8802..9146
                     /locus_tag="VF516_38810"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0048511 - rhythmic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEX8113767.1"
                     /translation="MKPAKNNSVRPRSAPRRARSKEYLLRLYVAGQTPRSVMALNNLK
                     AICEEHLAGRYEIEVIDLLVDPTLARGDQILALPTLIRRLPPPMKKIIGDLTSAEHVL
                     VGLDLRQRAGRK"
     gene            9150..9452
                     /locus_tag="VF516_38815"
     CDS             9150..9452
                     /locus_tag="VF516_38815"
                     /inference="COORDINATES: protein motif:HMM:NF019309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="circadian clock KaiB family protein"
                     /protein_id="HEX8113768.1"
                     /translation="MARSKAKRRPGKMVLRLYVAGTSERSMRAIVNARELLDAHIAGR
                     YELEVIDLFQQPTLARDDQIVAVPTLIKKLPEPLQRFIGDLSDKRVTIIGMDLRRR"
     gene            9457..>10054
                     /locus_tag="VF516_38820"
     CDS             9457..>10054
                     /locus_tag="VF516_38820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8113769.1"
                     /translation="MPARASRKKRSARRPRRASLAERLREAEETLEAIRGGHVDALVV
                     SGPHGEQVFTLRGADHRYRQLVETMNEGALLVSAVGLIVYSNSRFAMLVGAPLETLIG
                     SSLVAHAAEGSRKLVEALVGARGTGAVKAEVELVAADGTHIPVYMSATAAGDDSMNLT
                     SLVVTDLSAQKRDQEMLAAGRLSAHIVEHAAQGIVVCDR"
BASE COUNT         1616 a         3462 c         3519 g         1457 t
ORIGIN      
        1 attcagcccg atctggggag ccccgcacct gcgccgcgag acggcagccc acatcaccga
       61 cgcgatgcgc acgctgctcg tccgcgcggc cggctacgag gtgaaggcga tggagttcgt
      121 gcctgcggag cacacccgca agaacaccct gatccgcgcg gtcaagcgct ctgccggtga
      181 cgcggacgcc ctcgctgctt acgaggcgct gcgcaccgca acgggcggcg ccggcatcgc
      241 gctggcggcg cggctggccg cctccggata atcgccgcgc tcatccgggc gggacgctcg
      301 gctcccacgg cgccgggtgc aaacacccgg cccagagcgt cggcgatcgt ccaccgggcg
      361 cgcccgcgcg gtggcccgct gcatgccgac gagatgccgt gatttcgcca gggaattcgc
      421 cggaggatgt ccagcacgcg ccaggtcgtg atctggcggc gcaagcccga agccgagctc
      481 aagcctgccg tgaactgact ggcggtgctc acgctctccc cgcatcgtcg ggcctcaacc
      541 cggcgaaagg acgctctcga agccaggccc ggtgccagca aacgatcagg gaagccatct
      601 cgaagcatca cacgtcgtgg ttgggtccgg tgcgtcgcct acatggcggc cacgatcgtg
      661 cgggtagctg gcaataacgt gcagagtgag tttttgcagc aagcccgctg ctttgatttg
      721 cggcacacgt tcatgagccg gtcaccttgt cgcagacgcc gaccggttca tcatgcgagg
      781 gagcgaggct cgggaggcac gatgcgtgga gcgacggtgt tgggggtgtt ggtggtgatc
      841 accgcgcacg ccattcgccg gcgggcccgt ggcggagttc ggcgatgacg cggctgatgc
      901 atccgcccga gctcgaccgc ccacgagaga gcgatcggtt cgatccggtc gttgagggcg
      961 cccgactcgg cttgacgcgc gagctgtcgc tcgtgatctg gcagcggatc tgcgcagaca
     1021 tcagggacgc gcgggggcgg ctcgacgagg atcaggcacg ccagcagttc cgcgagctcg
     1081 cggcgcgcgt ggcagcgcgc ggtgggaggt tgcgccctga tgtcggccgg ctcacgcgcg
     1141 tggagatcga ggaccgagca gattccctgg acacctggtg cggcgacgcg ctgaccgccc
     1201 acaccccccg ggcgagacac gctcgtcgcg gtggaggagc ggcgccgggc gatgcagggc
     1261 gagcgcgcgc cgtcgaacga cgcgtcgcca ccggcaagcg cgccggcgcg tgaagtggcg
     1321 cgccgcgagc gctcgagccc ccgtgagatc gacgaacggc gcacggtgcc gtcccggatg
     1381 gagcgagcgt tcgggaccac gttcgccgac gtggtgatcc gcaccgacga gcgtgcagcg
     1441 gccagggccg cgtccctgga cgtgctcgcg tacgcggacg gccgcgagat cggcttctcg
     1501 cctgggtggt ggagcccgaa cacccccgag gggcagcgca cgctcgtgca cgagctcgcg
     1561 cagctggagc gtcctgcggc cgagttgatc gctcacgcgc cgacggtggc cgccgcgagc
     1621 ttgggctcca gccgcggatg ggagctgtgg gatcctcgcg ccgcccatcg cgacggctgg
     1681 cgactcccgc ccggcgaccg ccggaaggac gcacgcccgc tcgagtcgcg gcgcatcgcg
     1741 cctcggcgcg gggccctgga tggcttggtc gtgctcggca gctgggagcg gaccgagcac
     1801 gtgcgcgacg ccgaggcccg cctgcgcgag cgtgccgcgc tgctcgacgg cgtcgctgct
     1861 gactcgccgc ttgcacggct cggccgcgac ggcggcgagc cgctcgccga cgagctgcgc
     1921 gcgcgcatgg agcagctgtt cggccacggc ttcgcgcatg tccgcgtcca caccgacctg
     1981 gcagccgcgc aggcgacgag cgcgcttggc gcacgggcgt tcgccgtggg ccatcacgtg
     2041 tacttcggcg cggggttgtt ccagcccggc acctcgatgg cgatcgcctg atcgtccacg
     2101 agctcacgca cgtgaggcag cacgaccgcg gtgcactcgc gcgccccgat cgcgacgggc
     2161 tcggcgtgtc gcagccgggc gacgcggtcg agcaggaggc cgcggcaatg gagcactcgg
     2221 tgcaccgcgt acacccatcg cttgcgcccg atcgccaagc gcatcgtcga gcgcgcgacc
     2281 ttcgtgcaag gcgagctggt cgcggccctg atgctcgcct acagcgacgt cgatccggcc
     2341 ggccggcagc tcaccactgc gcagcgagac ctcggcgagt tcgcggactt cgtcatcgac
     2401 cagacgctgg gtcccgacgg cgtcgagcgt ctgatcgcac aggtcgtgcc gctgcgcgag
     2461 caaatcatca cggcgcgcgg tcagaccggc cgtgccggca tcacccggcc ggcggacctg
     2521 atggccgggt tcggcgagcc aaagcatatc ggcgggctcg agaagccgct gcgcaaggtg
     2581 ctcgacggct tgcgtcgctc gcacgcggcc ggaagcgacg tcggcgagaa gctgcggcat
     2641 ctgatcgagg acatccggtc ggtccacctg atcgagaaga tcatcgtcat caccgccgcg
     2701 gctgcgttca ccgccgggct cgccggcagc gcagcggcgg gcctggccgg cgaagtcggc
     2761 cttggcgggc tcgcgggcgc tgcgagcttc ggcgccgagg tcgtcgcgtt caccacagtg
     2821 agccgcatgg ccgagggcgc gatgatcggc ccctccgaga acagcttcgg gcacgacctg
     2881 gcgatcaatg ccctgacctt cggcgtgctc aagtcggtcg gccgcgtatt cgccaagatc
     2941 gtcaagctgg gcgccgatcc gtcgatgctg gccaaggccg cctaccgggc cggcagcttc
     3001 gccaccgtcg cggtcagcct gcagctgttc gccgaggccc agcacgtctt cgagcacggc
     3061 agcgcgatgt cgaacgagca gcgcgccagc tcgatcgcgc agaacgcgat cgtgatggcg
     3121 gcgctggagc tcggccactt catcgtcaag gcaccggcgg cgcgcggcct gggctcgatc
     3181 gatgcggcgc ttggcaagca gctggccgca ctcggcggcg agcgcgccgc catcgcctcc
     3241 cagctcgagg cgctgcgagc ccagaagctc acgccccagc aggtcaacga gctcctcgcg
     3301 aagatccagg atctgtacgg gcgcgagctt cagctgttcg acgcggccgc caagcgcggc
     3361 gccacgcagc cggccctgaa ggcggcgatc gcggtctacc agcagcagat cgcggccgtg
     3421 gagctcaagc tcgccagggc cggcgtggcc aatgcccaca ctgggccgca actattccag
     3481 caggttcgcc ccggcgtcgt cggcttccag cccgccgcgc gcccgatcat cgatcggttc
     3541 taccaggagc aaggcggtcg ggtcgtcgcg ggtcagcggc cgggggagct gatcggcacg
     3601 ctgcccgacg gcgagacgac gcactttctg tccgacgagc tggtcaagga cgagcagcgc
     3661 gcccgcgaac aggagcgcaa ggtcgaggca cgccgcaagc agctgctcga cgagatcggc
     3721 agcgcggtcg gcccggaggc attgccgcgg ttcaccgacc tgccgaccga gaccctcgag
     3781 cggctcgcca ggcgccccgc accggagctc gccaagctcg ccaggctcgc gcccgaagag
     3841 ctcgccaggc tgctcgcgct gcccgagctc cagctcggcc ggctgctcgg cctgccagcc
     3901 gaacagctca cggcgctggc caggctcgcg cctgatcagc tcgccaagct cggcggcctc
     3961 gagggccagt cgctcgtcaa cctggcgcgc cggacgccgg tcgagctgca gcgtatcctc
     4021 gcgctcgatc cggccgacgt gaagatcctc gccgagctga agttcggcca cgcgacggac
     4081 ctgctcggcg tatcgccgtc cccggcgcac ttgcgcgctg cggcgcgcgt gtgctcgcgt
     4141 ttcatggcag cggggatgtc cgagaaagcc gcggtcgatc gcaccgtgga gatcggcaca
     4201 agcggcgatc gcctactgct catgggtgac gcattccaag gcgggggccg cggcaccagc
     4261 aaacaggtgg tccacgccga cggcatcgag gcgcacgagg tggcgcaagc gatcgacttc
     4321 atccgcctca agggaggcgg cgggcagttc gagttcggcg cggtcggcct ggaaggcatc
     4381 gaggggcact acgtgcccga gggtgcgaag caacggatcc cggtctcgat gaaggtcttc
     4441 accaccgcgg acgccgtcaa cgttctacag cgagcagatc tcaatgcagg tcacgtggac
     4501 gccggcatcg gcgccgagct gttcgtctac acgaacgcgg acgcgaaggc catctacgac
     4561 ctggcgaaga ctccgccgcc cggcggtgct gccgcccagg tgttcaagcg catgaccttc
     4621 gtctgccgcg atgggacctt gatatacgat tctgggcgct ggcaaccgcc gagataggac
     4681 tcatggacac gatcatctac atcctgccgt cgctccacgt cccgacctcc tgggggatgc
     4741 tcgaggattc catccgccgt tggtcgggtg ccgacgcctc gcgcctgtgg gggccgacca
     4801 tcaacctgtc cgcgtggcgc gaagcagcac accgtcccga gttgccgcgc gacctcatcc
     4861 tggatgcgac ctatcgctac cggatcgatc tcgccggcga tgaccacggg ccctggatca
     4921 actaccaccc tcaccacctg ctcacgcacc cccggaatct attcggcgag atggggccca
     4981 cgttggggcc ctcggagatc gaccacatca tcaaggggat tcagcaaact ggctatttct
     5041 cacggtcctg acacgcccga accatcgcct gccgatcgag cgcctcaaga tcgtcgcctg
     5101 cgcaatggcc gagctggtcc acggccggct cggaaacttc caggatgaat tcgacgctct
     5161 gccggacggg atctactcac tcgacgaatt caaatatatg cagttgccat accgagccgc
     5221 acgcaggatc gacgagtaag ccgtttcgat cggtccgcga ggaggcctcg cctcacatca
     5281 aggccacact ccagcactac cacgaggtcg gatccttctg gaccggcgcg aaccgcagcc
     5341 agattccgcg gttcgtcctt ccatcgccgg ttcaactacg agatccggtc ggcggaagga
     5401 cgcatcttcg ttgaccgaat cgcggtctga tgccgcagcc agtctgcgcc gaccagcagc
     5461 aatgtaaagg ggtcgacacg cagccgtcga caggctggcg aggctcatcg gccccgctgc
     5521 gtcagaacgg ggggcgtcgt ttgcgacgga gcctgcgccg cagcagtgct actgtgtcgc
     5581 gcatgaagcg gctgtcgatc tcgctcctgt tcctggtgct gctcgcgtcg ccgagctcgc
     5641 cgcgctgccg cggttcctcg ccgatgcgca ggccgcgctg gcccacccgc gcggcaagcg
     5701 gcacaccccg cgcgtgtatc tcgaccaggc gatccagaac aaccgcggca cgatcgacct
     5761 ggtcaagggt gaggtccggc agttcgtcgg cgacgatccc gcggtcgagc gcgcccaggc
     5821 cgcgctcgag ggttaccaga ggttcctcga gaccgagctg gcgctgcacg ccgacggtga
     5881 ctagcggctc ggcgccgagc tctacggcaa gaagttcccg ctcgcgctgc agaccgatct
     5941 cacgccggcg cagctcgagg accgcgcccg ggccgcgttc gccgcggcgc gcgcacagct
     6001 ctacgtcgag tgcgagaagc tgcaccgcaa gctgtggcca aacgacgtgc tgccggccgg
     6061 cgtcaccgat ccggcggcgc agcacaaggt gatccagcgc gtggtcgacg agctcggcaa
     6121 ggaccacccc aggcccgatg agctcgtcgc cgcgcacggc cgcaacctcg atgcgctccg
     6181 cgcgttcatc cagaaccacg acctcgtggc gctgccgccc cgcgacacgc tgcgcgtcga
     6241 gccgatgccc gcgttcaagc gcggctcgac cgccgccgag taccttgcgc ccggcgtgct
     6301 cgtccgcaat ccgaactggc gcgccacgta ctatgtcgat ccgatcgatc cgtcgtggcc
     6361 gccggaccgg gtcgaatcct acctgcgcgg ccagaacgat tacgtcgtgc agctggtcgc
     6421 ggcgcacgag gcctatcccg gacaccacac gcagtacttc cactccaagc gcaacctgaa
     6481 cccgctgcgc gccgtgctgt ggaacggccc gatggtcgag ggctgggcgg tctacggcga
     6541 gggcctcatg gtcgggctcg gctggggcgg cgagcgcaac gaccgcttcc gggtgttcga
     6601 gctcgtcgag atcctggtgt cgaccgccaa cacgatcatc gacatcgagc tccagtccgg
     6661 gcggatgacc gacgaggcgg ccgtgcggtt catggtcgag gacggctttc agccgcgcgc
     6721 gcaggccgag aagaagctgg tccgcgccaa gctcgattcg acgcagctct gccagtacta
     6781 cctcgggctc gacgagatcc ggtcgctcga gcgcgattac aaggcccagc tcggtgcccg
     6841 gttcaagcag cgcacgttcg acgaggcgct gatcggccac ggctcgctgg ccgtgaagtt
     6901 cctgcgcgag tacctgctcg gcgccggccg gtgaccgggt cccgcgagct cggaaggcgg
     6961 tggtgccttc cgtcctgatc gaggtgggcg caggcgcaca tgtgcgcgtg cgctcgctgc
     7021 atcggtccgc tcgcgttgtg cgttgacccc agcgcacaag atcgctaagt ccggagcgca
     7081 atgccgacgc gtaggcggcg agcacccagc gcggggctcg cgaagtctcc ttctggaatc
     7141 gacggattcg atcggatcac gcagggcggc ttgcccagcg ggcgcacgag cctcgtgtgc
     7201 ggcggcgctg gatgcggcaa gacgctgttt gcgatcgagt tcctggtcaa gggcgcgctg
     7261 ctctacgacg agccgggagt gttcgtcgcg ttcgaggaga cccaggagga gctgatccgc
     7321 aacgtcgcat cgctcggcat ccgcctcgac gagctgatcg cgcggaagct gatcgcgttc
     7381 gacttcatcc gcgtcgagcg cagcgagatc gaggaggcgg gcgagtacga tctcgagggg
     7441 ctgttcgtgc gactcgccca cgcggtgtcg tcgatcaagg cgcggcgcat cgcgatcgac
     7501 tcgctcgagg cgctgttctc gacgttcgcg aacgaggcgc tgctgcgcgc cgagctgcgg
     7561 cgtctgttcc gctggctcaa ggacaagaag ctcacggcgg tcatcacggg tgagaagggc
     7621 gagggggcgc tcacgcgaca cgggctcgag gagtacgtct cggactgcgt ggtcgcgctc
     7681 gatcaccgcg tcgtcgacca gtcctcgacg cggcgcatgc ggatcgtcaa gtaccgcggc
     7741 agcgcgcacg gcaccgacga gtatccgttc ctcatcgacg agaagggctt ctcggtcctt
     7801 ccggtcacga gcctcgacct cgactacgag gtctcgaccg agcgcatctc gacggggatc
     7861 gcgtcgctcg acgggatgct cgagggcggc ggctacttcc gcgggtcgag cgtgatggtg
     7921 tccggatctg ccggtaccgg caagaccagc ctggcggcgc tgttcgccgc ggcgtcgtgc
     7981 aagcggcgcg agcgaacgct ctacttcgcg atggaggagt ccgcctcgca gatccagcgc
     8041 aacatggcgt cgatcgggtt cgacctcgca gagctgcgga ggaagaagat gctcgacctg
     8101 cgcgccgctc gccccacggt gtacgggctc gagatgcacc tggtcgcgat gcacaacgcc
     8161 atcgaggagt tcgagccccg cgcggtgatc atcgatccga tctcgagcct gagcgccacg
     8221 ggcacgcagc gcgaggtgaa ggcgatgctc gtgcggctgt tcgactacct caagaacaag
     8281 cagatcacgt gcctcgtcac gtcgctggcg gcggcgcaag ttggcgagca gaccgagctc
     8341 gagatgtcct cgctgatcga cacctggatc caggtgcgcg acctcgagct cgcgggcgag
     8401 cggacgcgcg ggctgttcct catcaaagcg cgcgggatgg gacactccaa ccaggtgcgc
     8461 gagttcgtga tcgggtcgca tggcatcgac ctgatccccg tcgagatcgg tcccagcgga
     8521 gtgttgaccg gttcggcgcg cgagaaccgc gagaacgagc gccgcaccga ggcgatggcc
     8581 cgggagcacg agcgcgagcg caagctgcgt cagctcgagc gccggcgcac cgccgtggaa
     8641 gcgcagatcg cagcgatgcg cgcggagctc gaagccgagg cctccgagct cgaggcggcg
     8701 gcgagcgaag agcgtgagcg cacgcggcgc acgtccgagg acgaggcgcg ccggggggcg
     8761 acgcgcagcg ccggacgcgc cacgaccaac ggagagcgca ggtgaagcct gcgaagaaca
     8821 acagcgtgcg gccgcggtcc gcgccgcggc gtgccaggag caaggagtac ttgctgcgcc
     8881 tgtacgtcgc cgggcagacg ccgcggagcg tgatggcgct caacaacctg aaggcgatct
     8941 gcgaggaaca cctcgccggc aggtacgaga tcgaggtgat cgacctgctg gtcgacccga
     9001 cgctggcccg cggtgatcag atcctcgcgc tcccgacgtt gatccgtcgc ctgccgccac
     9061 cgatgaagaa gatcatcggc gatctcacga gcgccgagca cgtgctcgtc ggcctcgacc
     9121 tgcgccagcg tgcgggacgg aagtgatcga tggcgcgaag caaggcgaag cgtcgtccgg
     9181 gcaagatggt cctgcgcctc tacgtcgcgg gaacctccga acggtcgatg cgcgcgatcg
     9241 tgaacgcgcg cgagctcctc gacgcacaca tcgcgggccg ctacgagctc gaggtcatcg
     9301 atctgttcca gcagccgacg ctcgcgcggg acgaccagat cgttgcggtg ccgacgctga
     9361 tcaagaagct cccggagccc ctccagcgct tcatcgggga tctctccgac aagcgcgtga
     9421 cgatcatcgg gatggacctg cgacggaggt gatgccgtgc ccgcgcgcgc ctcgaggaag
     9481 aaacgatccg ccaggcggcc ccggcgagcg agcctcgccg agcgactgcg cgaagccgag
     9541 gagacgctcg aggcgatccg cggcgggcac gtcgacgcgc tcgtcgtgag cggcccgcac
     9601 ggcgagcagg tcttcacgct gcgcggtgcg gatcaccgct atcgccagct cgtcgagacg
     9661 atgaacgagg gcgcgctgct ggtcagcgcc gtcgggctga tcgtgtacag caactcgcgc
     9721 ttcgcgatgc tggtcggtgc gccgctcgag acgctgatcg gctcgtcgct cgtggcgcat
     9781 gcggcggagg gctcgcgcaa gctcgtcgag gcactcgtgg gcgcgcgcgg caccggcgcg
     9841 gtcaaggccg aggtcgagct ggtcgccgcc gacggtacgc acatccccgt gtacatgtct
     9901 gcgacggccg cgggcgacga cagcatgaac ctcacgtcgc tggtcgtgac cgacctgagc
     9961 gcgcagaaac gcgaccagga gatgctcgcc gcgggccggc tgagcgcgca catcgtcgag
    10021 cacgcggcgc agggcatcgt cgtgtgcgac cggg
//
LOCUS       DASVOR010002089         2844 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_284153,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002089 DASVOR010000000
VERSION     DASVOR010002089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284153"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1990
                     /locus_tag="VF516_38825"
     CDS             <1..1990
                     /locus_tag="VF516_38825"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8113770.1"
                     /translation="LSPLVGGTAWYAAGVAPRLGTRDVAWRAHRVVDTDGNGVPDGLP
                     GDVVVAPIEDTQTRELDYFVPVMARVGADFGAHHVALSLVGDVARDSRFLANSTLQAA
                     GVDRRTFAGDAIATWEGQWSDVHARAQLTWHRSSRTESARDDAAAGMPQLLSAYVPAH
                     LAEDPALAAVCDDGSPSDPAPTVPNCPVPFGFFASGGAGPLVDQVGDRPTATADVTYR
                     IADHVVRAGATVEDSRLVTTTRYTGGELDRSLLEFPALIRRRFYLGDCSDTPGGPCAY
                     ADSSQLVYRTVYAAAYAEDTYTPIDGLAIDAGVRWELMWVGPRLHFSHELAPRLGVVW
                     DPLGGGKSRLWASYGRTFALLPPGLGATVIQRDTTVDDTQLGTLTARNRDAGAAFRIV
                     PGTDPIEQDELTVGGEVALAGALRATAWGQSRTLHHGLETTADGFTNPGADGASPATR
                     ETQLVAFQLELRKQDTTAIRAGVMWGRTVGTWTGPFDPRLGANQLNAPDWDVDASNLY
                     GPLPTSPGTRMFLEAERRFTLGELGFAIATRLTAGSGRPRNVLANGTDGIVELLPRGS
                     AGNNPVVSQANLRLAARWAGFDFTLDVINLFDRRTPTNLDEIYTTDPVRPVVGGSASD
                     LVLVKTDDGAPAHRLTSFQLPTAYQPPLSVTLGVHKAF"
     gene            2113..>2844
                     /locus_tag="VF516_38830"
     CDS             2113..>2844
                     /locus_tag="VF516_38830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113771.1"
                     /translation="MADLSQLQEAAAAVRHSPHAVSAWEEAEGLAAELDRPDDIVRLY
                     NDVLRGAVEPEVAEMIGERAGGFCDEWFGDDPATLENILVRVIELAPASDTALQRLSV
                     IYTVAERWTDALGLYDRAVDATKDKARRVRLLREAAQLAKDVANQPDKAIGYYQRLLP
                     LAPEDGQVSQSLERLLERHERWPDLIALWESRLDGLSKRDRERSRARIAAVWLDNLGD
                     PQRALAAAKPLLSEADDDRESTALLE"
BASE COUNT          381 a         1086 c         1023 g          354 t
ORIGIN      
        1 gctgtcgccg ctggtcggcg gcaccgcgtg gtacgccgcc ggcgtcgcgc cgcggctcgg
       61 cacccgcgac gtcgcgtggc gcgcccaccg ggtggtcgac accgacggca acggtgtccc
      121 cgacgggctg cccggcgacg tcgtggtcgc gccgatcgag gacacgcaga cccgcgagct
      181 cgactacttc gtgccggtca tggcgcgggt cggggccgac ttcggcgcgc accacgtcgc
      241 gctgtcgctg gtcggcgacg tcgcgcgcga ttcgcggttc ctcgccaact ccacgctgca
      301 ggccgccggt gtcgaccggc ggacctttgc cggcgacgcg atcgcgacct gggagggcca
      361 gtggtccgac gtccacgccc gggcccagct cacctggcac cgcagctcgc gcaccgagtc
      421 ggcgcgcgac gacgccgccg ccggcatgcc gcagctgctc agcgcctatg tcccggccca
      481 cctggccgag gaccccgcgc tcgccgcggt gtgcgacgac ggatcgccga gcgacccggc
      541 gccgaccgtc ccgaactgcc cggtgccgtt cgggttcttc gcgagcggcg gcgcgggccc
      601 gctggtcgac caggtcggcg accggccgac cgcgaccgcc gacgtcacct accggatcgc
      661 cgatcacgtc gtgcgcgccg gcgcgacggt cgaggacagc cggctggtga ccacgacccg
      721 ctacaccggc ggggagctcg accggtctct gctggagttc ccggcgctga tccgccggcg
      781 gttctaccta ggcgactgca gcgacacgcc gggcggcccg tgcgcctacg cggactcgtc
      841 gcagctggtc tatcgcaccg tgtacgcggc ggcctacgcc gaggacacgt acacgccgat
      901 cgacgggctc gcgatcgacg ccggcgtgcg ctgggagctg atgtgggtcg ggccgcggct
      961 gcacttctcg cacgagctcg cgccgcggct cggcgtggtg tgggacccgc tcggcggcgg
     1021 caagtcgcgg ctgtgggcga gctacggccg gacgttcgcg ctcttgccgc ccgggctcgg
     1081 cgccacggtg atccagcgcg acaccacggt cgacgacacc cagctcggca cgctcaccgc
     1141 ccgcaaccgc gatgccggcg cggcgttccg catcgtgccc ggcaccgatc cgatcgagca
     1201 ggacgagctc acggtcggcg gcgaggtcgc gctcgccggc gcgctgcgcg cgaccgcgtg
     1261 gggccagagc cgcaccctgc accacggcct ggagaccacg gccgatggct tcaccaaccc
     1321 gggcgcggac ggcgcatcgc cggcgacccg cgagacccag ctggtcgcgt tccagctcga
     1381 gctccgcaag caggacacca ccgcgatccg cgccggcgtg atgtggggcc gcacggtcgg
     1441 gacatggacc ggtccgttcg atccgcggct gggcgcgaac cagctcaacg ccccggactg
     1501 ggacgtcgac gccagcaacc tctacggccc gctgccgacc agcccgggca cccggatgtt
     1561 cctcgaggcc gagcgccggt tcacgctcgg cgaactcggg ttcgcgatcg cgacccggct
     1621 gaccgcgggc agcggccggc cgcgcaacgt gctcgccaac ggcaccgacg gcatcgtcga
     1681 gctgttgccg cgcggctcgg cgggcaacaa cccggtggtc agccaggcca acctccggct
     1741 ggcggcgcgc tgggcggggt tcgacttcac gctcgacgtg atcaacctgt tcgaccgccg
     1801 caccccgacc aacctcgacg agatctacac cacagatccg gtccggccgg tggtcggcgg
     1861 aagcgcgagc gacctggtgt tggtcaagac cgacgacggt gcgccggcgc accggctgac
     1921 ctcgttccag ctgcccacgg cctaccagcc gccgctgtcg gtcacgctcg gcgttcacaa
     1981 ggcgttctga cccgcccgag ccggccggtc cgagccgggc gcccgcgccc tgtgcgcgga
     2041 cgcgcatgtt accatcgccc tccgctgccg cgcggtccgc ggcgcgagtc gccgcgtcca
     2101 cccggggaat tcatggccga tctgtctcag ctccaggaag ccgccgccgc cgtccgacat
     2161 tcgccgcatg ccgtgagcgc gtgggaggag gccgaggggc tcgccgccga gctcgaccgg
     2221 cccgacgaca tcgtccggct gtacaacgac gtgctgcgcg gcgccgtcga gcccgaggtc
     2281 gccgagatga tcggcgagcg cgcgggtggg ttctgcgacg agtggttcgg cgacgacccg
     2341 gcgacgctgg agaacatcct ggtccgcgtc atcgagctgg cgccggcgtc ggacaccgcg
     2401 ctgcagcggc tgagcgtgat ctacaccgtc gccgagcgct ggaccgacgc gctcgggctg
     2461 tacgaccgcg cggtcgacgc gaccaaggac aaggcgcgcc gggtccgcct gctgcgcgag
     2521 gccgcgcagc tcgccaagga cgtcgcaaac cagcccgaca aggcgatcgg ctactaccag
     2581 cggctgttgc cgctggcgcc ggaagacggc caggtcagcc agagcctcga gcgcttgctc
     2641 gagcgccacg agcgctggcc cgacctgatc gcgctgtggg agagccggct cgacgggctg
     2701 tccaagcgcg accgcgagcg cagccgggcc cggatcgccg cggtgtggct cgacaacctc
     2761 ggcgacccgc agcgcgcgct ggcggccgcc aagccgctgc tcagcgaggc cgacgacgac
     2821 cgcgagtcga ccgcgctgct cgag
//
LOCUS       DASVOR010002090         6823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_167992,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002090 DASVOR010000000
VERSION     DASVOR010002090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6823
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167992"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(380..1333)
                     /locus_tag="VF516_38835"
     CDS             complement(380..1333)
                     /locus_tag="VF516_38835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113772.1"
                     /translation="MVAASMRTRYDQFCKQMIRTELETCGPIETDAEVPADTRRIDLW
                     FVRDPTRGPMPDHLGLLGRIASSSSTFEFFHNTPSGEDLADCLIKHGQFRHFLSLRGV
                     PPPVPIQWVISSGRPDGGIDGLWLRPIHGWPPGFYEGPPLLWTRLVVVNELPVARDTL
                     LLRLLGAGAVLRQAIAELKELRAEAPERTLALPILLRLRLTVPSDPAKQTTDDQEFLM
                     DTQDIVETWRREAIEEGIVRGREEGIVRGREEGVVRGREEGVAHSLVEVYQARFGAMP
                     DDLRAVVEDTDDEPTLITWLRLASTRSADEIAAAIRSFRPS"
     gene            complement(1750..3192)
                     /locus_tag="VF516_38840"
     CDS             complement(1750..3192)
                     /locus_tag="VF516_38840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113773.1"
                     /translation="MAGCHPGDDTAAIVQAVGPPAPEVMTDADTVLKYATACGLGDTP
                     DMDLPDLGDKTTPIRGTTDATQVGKLTKKGWTLVQHFDADTAHLASGAHLKANLWHKQ
                     KGGRDWYYLYRYDDPDPTVKQENANGILGFDATSICAFDKLAPAATPALPPASISDLY
                     FAGQFDNNPAKMDDCSDCHLAGYNAPRPKTLAVAKGDNGKMPWLKIKWLPQWMKFTNA
                     YGPVWKLGKATKTDKWTSGDGMALVTPPADCSDCHKKWIPVRNRDDGAYCDTVFAKAF
                     DGNGSMTKQKSTFGDNATCTTFITDIGCGPGRPGGAADLRTLCPPAKPPAPMVSDGRM
                     MIQGIQVVSSTAVNIVPAQDSTVTWDLGSVFAFGDAWVTSIQVWGGPYPGSTTFPMSP
                     VINVPAPDVLPSFIQVTGLAPGQSYQFQLRVTDSDGAIGFEPLTMVTMPLDGNGSGSG
                     SGGGSDAGVDSGSGSDAGAGSGSGSGSGVG"
     gene            complement(3644..5137)
                     /locus_tag="VF516_38845"
     CDS             complement(3644..5137)
                     /locus_tag="VF516_38845"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8113774.1"
                     /translation="MMQRRTFLHGAAVVGAAALLPRRGHAAANLKPVYDQIEKRRAET
                     IARIQEWIKQPTIAAESRGITEGCDLTMRLLRDAGFGKVERVPTDGHPGIFATLDAGA
                     RRTIGLYFMYDVKQVDPKEWSSPPWDAAIVDVPKIGKSIMGRGAVNQKGPQATFLAAL
                     HAIRGAGRKLPVNFVFVAEGEEEIGSPHFSQIVSRPDIRAALKRCDGIFMPHAGQSSD
                     GSVTINLGAKGIVECEIVSSADTWGRGPKRDVHSSNRARIDSPAWHLVQALNTLVNAE
                     GDPAIDGLFERVKPLSERDRKLLDDAARQMNEAEAKASLGVQRWAHDASWRQALDDLT
                     SKPTVNIEGIYGGYTGPGGMTILPHRAAAKLDLRLVPDMTAADTFAKLKAHLAKRGYG
                     DLDIKMTGGYDPNTTPADSRLIQAQVKVYEQAGIKPILWPRLAGSWPGYVFTGEPLKL
                     PAGHFGLCHGSRAHAPDEYYLIEPSDPRLHGLDAAVRGFVDYLHELA"
     gene            5172..5501
                     /locus_tag="VF516_38850"
     CDS             5172..5501
                     /locus_tag="VF516_38850"
                     /inference="COORDINATES: protein motif:HMM:NF018717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HEX8113775.1"
                     /translation="MALYALNLFDLADNDDYLAYSRRSVAAVGKYGGKVVALGRLDSE
                     ATPDRGDTAPRQVMVLVEWPDRAAFEAFQDDPAHHDLHPLRRHGTRNYLWWAYERLED
                     LRPLLKR"
     gene            5516..>6823
                     /locus_tag="VF516_38855"
     CDS             5516..>6823
                     /locus_tag="VF516_38855"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8113776.1"
                     /translation="MRSALVLAVILASILASILALILAFGGACAGGCTPPASEPAPGA
                     EAGVRYVSIGRDALDAALAVAERRGAPLQVLDLDADVAVIALDARDLGLLAREVHARH
                     DRCGGFMVHDSYADARAAIPVHSDPDHADPSHAVRPELDYTIDRPAAVHAVLPAVDAT
                     RIRDTIRALSAMPTRYYQSRTGAEAARWLQDRWRSLTGRPDVTVELVDHGYAQPSVIL
                     TIPGTARAGEVVVIGGHLDSINLLGSAVAAPGADDDASGIATVTEVARVLLAHDYRPA
                     RTIQFMAYAAEEVGLRGSQLIARDYQRRGVRVVGALQLDMTNYRGSDKDIWLINDFTS
                     AAQNAFLGRLIDTYVGATWGMDACGYACSDHVSWHRIGVPASMPFEARAGDRNRAIHT
                     RRDTLETSDSSAEHAARFARLAAAYAIELGKGELGKGEPGKGDG"
BASE COUNT         1019 a         2337 c         2369 g         1098 t
ORIGIN      
        1 ggagctggtc ccaggcgtcg tgggtggggg tgggttccga catcccccat cacccacgca
       61 atcggccggc gatctcgaca acgactagat aactattagc actcgctggg atttttcggg
      121 cgccgggcgc cgctggcgcg gcggcgtcgc ggctgggcgc gcaggctcca gatcgccgcg
      181 ctgcgcagat gtcggggggc attgctgcca ggctagacgg tcgcgacctc gaccaccatt
      241 acggtggatt accgccgccc ggctctcgac ctcgccgcac gagggacttg acgcggtccc
      301 gcgaacccat gcagcggtgg aacgcccgat cagaccagga ggcatgatct cgccgcgcat
      361 cgccggaact tcggtcgact cagctggggc ggaacgaacg gatggctgcg gcgatctcgt
      421 cggcactgcg ggtgctggca agccggagcc aggtgatgag cgtgggctcg tcgtcggtgt
      481 cttctaccac cgcgcggaga tcgtcgggca tcgcgccgaa ccgtgcctgg tagacctcga
      541 ccagcgaatg tgcgacgccc tcctcgcggc cccgcacgac gccctcctcg cggccccgca
      601 cgatgccctc ctcgcggccc cgcacgatgc cctcctcgat cgcctcgcgg cgccaggtct
      661 cgacgatgtc ttgggtatcc atgagaaact cctgatcgtc ggtcgtctgc ttcgcaggat
      721 cgctcggcac ggtcaaccgt aagcgtagca gaataggaag cgccagcgtg cgctccggcg
      781 cttccgcccg cagctccttg agctcggcga tcgcctgcct gagcactgcg ccggcgccga
      841 gcagccgcaa gagcagcgtg tcccgcgcaa cggggagctc gttcaccacc accagccggg
      901 tccacagtaa cggcggccct tcgtagaaac ccggtggcca gccgtgtatc gggcgcaacc
      961 acaggccgtc gatgccgccg tccggacggc ccgacgagat cacccactgg atggggaccg
     1021 gcggcggcac gccgcgcagc gagaggaagt gccggaactg gccgtgcttg atcaggcaat
     1081 cggcgagatc ctccccgctc ggtgtgttgt gaaagaactc gaacgtgctc gagctgctcg
     1141 cgatgcgtcc gagcaggccg aggtgatcgg gcatcggtcc gcgcgtcggg tcgcgcacga
     1201 accagaggtc gatccggcgc gtgtccgccg ggacctcggc gtcggtctcg atggggccac
     1261 aagtttccag ctcggtgcgg atcatctgct tgcagaattg atcatagcgg gtacgcatcg
     1321 acgcggcgac catcgcacac cgaattgtca ggacaaagaa actggatatg agtgcagctg
     1381 aaaagacact cacgtgagcg tcgagctcgg atcgaattcc gatgcttcgc gcgcaccggc
     1441 ctcgatgtgg cgggccgcgc gtcgggccgc gacctggact cgcggggttg cggcttgtcc
     1501 ggttgtccgg agtgtccgct cgccggacag cgtgcaccga tcggccgaga accgggcgcg
     1561 caaacgaacc ccggtcggga tcttcccccg gatcttcccg gcgtcgggtc cgtgcagcta
     1621 gggatggccg tcgctgatga cgagccgcgg cgggcgctca gcgcgtccag cgctggttcg
     1681 cgccgccgtt gcacgtccag agccgcggag ctggaacccg agtctgtgcc cgtatcctag
     1741 gccgagcctc tacccgaccc ccgagccgga gccgctcccc gagccagcac cggcgtccga
     1801 gccgctcccc gagtcgacac cggcgtccga gccaccaccg gagcccgagc ccgagccgtt
     1861 gccgtcgagg ggcatggtca ccatggtgag cggctcgaag ccgatggctc cgtcgctgtc
     1921 ggtcacgcgg agctggaact ggtagctctg gccgggcgcg agacctgtga cctggatgaa
     1981 ggacggcaac acgtcgggcg cggggacgtt gatcaccggg ctcatcggga aggtcgtcga
     2041 gccgggatag ggacctcccc agacctggat cgaggtgacc caggcgtcgc cgaacgcgaa
     2101 caccgagcca agatcccagg tcacggtcga atcctgtgcc gggacgatgt tgaccgccgt
     2161 cgagctgacg acctggatgc cctggatcat catgcggccg tcgctgacca tcggcgccgg
     2221 cggcttggcc ggcgggcaca gggtcctgag atcggcggcg ccgccgggtc gacctgggcc
     2281 gcaaccaata tcggtgatga aggtcgtgca ggtcgcgttg tcgccgaacg tgctcttctg
     2341 cttggtcatg ctgccgttgc cgtcgaacgc cttggcgaac accgtgtcgc agtaggcgcc
     2401 gtcgtcgcgg ttgcgcaccg ggatccactt cttgtggcag tcgctgcagt cggcgggcgg
     2461 cgtcacgagg gccatgccgt cgcccgaggt ccatttgtcc gtcttcgtgg ccttgccgag
     2521 cttccagacc ggcccgtagg cattcgtgaa cttcatccac tgcggcagcc atttgatctt
     2581 cagccaaggc atcttcccgt tgtcgccttt cgcgacggcg agcgtcttgg gccgaggtgc
     2641 attgtagcca gccaggtggc aatcggagca atcatccatc ttcgcagggt tgttatcgaa
     2701 ctgtcctgcg aagtagagat cgctgatgct cgcgggcggc agtgcgggcg tggcggcagg
     2761 cgcgagcttg tcgaacgcac agatcgatgt cgcatcgaac ccgaggatgc cattcgcatt
     2821 ctcttgcttg actgtcggat ccggatcgtc gtaccgatat agataatacc aatcgcggcc
     2881 gcccttctgc ttgtgccaca gattcgcctt gaggtgagct ccggatgcga ggtgcgccgt
     2941 gtcggcatcg aagtgctgga ccagggtcca gcccttcttc gtgagcttgc cgacctgggt
     3001 ggcgtcggtg gtgccgcgga tcggagtggt cttgtcgccg aggtcgggaa ggtccatgtc
     3061 cggcgtgtcg ccgagtccgc acgccgtggc atacttcagg acggtgtcgg cgtccgtcat
     3121 cacctcgggg gccggcgggc cgaccgcctg gacgatggcc gccgtgtcgt cacccggatg
     3181 acagccggcc attcccagga tcgtgaacag tacgtgtggc gaataccgac aatagaacca
     3241 cctgtcgcga gagttgggat catatcgcat gacgctctcc ttcgagagag cgcgactcgc
     3301 aagcggtatg ccggcagcgc catagataac tcgcaggaac catcaggtcg cgctcggtgg
     3361 ctgctgcagg ctgccgcaag cagtgcaggc cggcacctgc agcggtgacc ggcagcgccg
     3421 tgggcgagac catcgcaaca agaagcttca gccctttcga ctgtctagga cgaagccgat
     3481 tgcgaagatc gaagctgggc agcggaggcg tccagcgaat cttcgaggac gctctgttgc
     3541 cggcagcaat ggagaatgtc gccaggagcc cgaagatcgc agtcgccaca acaacgggac
     3601 cactgccgcc aatctgagag caggtggcga gcgcagcggc gggctacgcg agctcgtgca
     3661 ggtagtcgac gaagccgcgg accgcggcgt cgagcccgtg cagcctggga tcgctcggct
     3721 cgatcaggta gtactcgtcg ggggcgtgcg cgcggctgcc gtggcacagg ccgaagtgac
     3781 cggccggcag cttgagcggc tcgccggtga acacgtagcc cggccacgag ccggcgagcc
     3841 gcggccacag gatcggcttg atcccggcct gctcgtagac cttgacctgg gcctggatca
     3901 gccgggagtc ggccggggtc gtgttcgggt cgtagccgcc ggtcatcttg atgtcgaggt
     3961 cgccgtaccc gcgcttggcg aggtgggcct tgagcttggc aaacgtgtcg gcggcggtca
     4021 tgtcggggac caggcggagg tcgagcttgg cggcggcgcg gtggggcagg atggtcatgc
     4081 cgcccgggcc ggtgtagccg ccgtagatgc cctcgatgtt gacggtcggc ttgctggtca
     4141 ggtcgtcgag cgcctggcgc cagctcgcgt cgtgcgccca gcgctggacg ccgagcgatg
     4201 ccttggcctc ggcctcgttc atctgccggg cggcgtcgtc gagcagcttg cgatctcgct
     4261 cgctcagcgg cttgacccgc tcgaacaggc cgtcgatcgc cggatcgccc tcggcgttga
     4321 ccagcgtgtt cagcgcctgc accaggtgcc acgccgggct gtcgatccgg gcgcggttgc
     4381 tggagtggac gtcgcgcttg ggcccgcggc cccaggtgtc ggcgctcgac acgatctcgc
     4441 actcgacgat gcccttggcg ccgaggttga tcgtgaccga gccgtcgctg ctctggccgg
     4501 cgtgcggcat gaagatgccg tcgcagcgct tgagggcggc ccggatgtcc gggcgcgaca
     4561 cgatctgcga gaagtgcggc gagccgatct cctcctcgcc ttcggcgacg aacacgaagt
     4621 tgaccggcag cttgcggccg gcgccgcgga tcgcgtggag cgcggcgagg aacgtcgcct
     4681 gcgggccctt ctggttgacc gcgccgcgcc ccatgatcga cttgccgatc ttggggacgt
     4741 cgacgatcgc ggcgtcccac ggcggcgagc tccactcctt gggatcgacc tgcttgacgt
     4801 cgtacatgaa gtacaggccg atcgtgcgcc tggcgccggc gtcgagcgtc gcgaagatgc
     4861 cggggtggcc gtcggtcggc acgcgctcga ccttgccgaa cccggcgtcg cgcaagagcc
     4921 gcatcgtcag gtcgcagccc tcggtgatgc cgcggctctc ggcggcgatc gtcggctgtt
     4981 tgatccactc ctggatccgc gcgatggtct cggcgcgccg cttctcgatc tgatcgtaga
     5041 ccggcttgag gttggcggcc gcgtggcccc gtcgcggcag cagcgcggcg gcgccgacga
     5101 ccgccgcgcc atgcaggaag gttcggcgtt gcatcatggc gcgcgactat acaccccggt
     5161 acgttcccgc catggcgctc tacgcattga acctgttcga cctggccgac aatgacgact
     5221 acctggcgta ctcccggcgc agcgtcgccg cggtcggcaa gtacggcggc aaggtggtcg
     5281 cgctcggccg gctcgacagc gaggcgaccc ccgaccgcgg cgataccgcg ccgcgccagg
     5341 tcatggtgct cgtcgaatgg ccggatcgcg cggcgttcga ggcgttccag gatgacccgg
     5401 cgcaccatga cctgcacccg ctgcgccgcc atgggacccg caactacctg tggtgggcgt
     5461 acgagcggct ggaggacctt cgcccgctgc tcaagcgctg agctacactg caggcatgcg
     5521 cagcgcgctc gtcctcgcgg tgatccttgc gtcgatcctc gcctcgatcc tcgccttgat
     5581 cctcgccttt ggcggcgcgt gcgccggcgg ttgcaccccc ccggcatcgg agccggcgcc
     5641 cggcgccgag gccggcgtgc gctacgtgtc gatcggccgc gacgcgctcg acgcggcgct
     5701 cgcggtcgcc gaacgccgcg gcgcgccgct gcaagtgctc gacctcgacg ccgacgtcgc
     5761 ggtgatcgcg ctcgatgctc gcgatctcgg cctgctcgcc cgagaggtcc atgcgcgcca
     5821 cgaccgctgc ggcggcttca tggtgcacga cagctacgcc gacgcccgcg ccgcgatccc
     5881 ggtccatagc gaccccgacc acgccgatcc gagccacgcc gtccgccccg agctcgacta
     5941 cacgatcgat cgccccgcgg cggtgcacgc cgtgctgccg gcggtcgacg ccacccggat
     6001 ccgcgacacg atccgcgcgc tgtcggcgat gccgacccgc tactaccagt cgcggaccgg
     6061 cgcggaggcc gcgcgctggc tgcaggaccg ctggcggagc ctgaccggcc ggccggacgt
     6121 cacggtcgag ctggtcgacc acggctacgc gcagccgtcg gtgatcctca ccatccccgg
     6181 cacggcccgc gccggcgagg tcgtcgtgat cggcggccac ctcgactcga tcaacctgct
     6241 cggctcggcg gtcgcggcgc ccggcgccga tgacgatgcc tcgggcatcg ccacggtcac
     6301 cgaggtcgcg cgcgtcctgc tcgcgcacga ctaccgcccg gcgcgcacga tccagttcat
     6361 ggcgtacgcc gccgaggagg tcggcctgcg cggctcgcag ctgatcgcgc gcgattacca
     6421 gcgccgcggc gtgcgcgtcg tcggcgcgct ccagctcgac atgaccaact accgggggtc
     6481 cgacaaggac atctggctca tcaacgactt caccagcgcg gcgcagaacg cgttcctcgg
     6541 ccggctgatc gacacctacg tcggtgcgac ctgggggatg gacgcctgcg gctacgcctg
     6601 ctcggaccac gtgtcgtggc accgcatcgg ggtcccggcc tcgatgccgt tcgaggcgcg
     6661 ggccggcgat cgcaaccgcg cgatccacac ccgccgcgac acgctggaga ccagcgacag
     6721 cagcgccgag cacgccgccc ggttcgcgcg gctcgccgcc gcctacgcga tcgagctcgg
     6781 caagggcgag ctcggcaagg gcgagcccgg caagggcgac ggt
//
LOCUS       DASVOR010002091         2807 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_168004,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002091 DASVOR010000000
VERSION     DASVOR010002091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2807
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168004"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="VF516_38860"
     CDS             complement(<1..284)
                     /locus_tag="VF516_38860"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEX8113777.1"
                     /translation="MAEHRINHLEVELHGASVPVARAVSERLGQIHGRRVVPVLDRVC
                     EELAAGEELVRLDRLEVDLGTLPADGFEDGFVARLEQALRAALAQALRRRG"
     gene            complement(289..>2807)
                     /locus_tag="VF516_38865"
     CDS             complement(289..>2807)
                     /locus_tag="VF516_38865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113778.1"
                     /translation="RAWLDGHVEAGDPRERRKRFLGHLLARYAEELGDHARIAPQGRA
                     ADPDDLLIADRSAFLRAYPALSRGRGSGYDLSPGASDAGGFEDRLRHKLGLRDGERLY
                     VVEHVLLRPVLEDKLPAADEDEPDVPLLAGVSGPDPWSLHVSVVIAELDPPDPHGSFE
                     QLVEQTVLAEAPAHLSVDLQWLGKTDGVDDWAAFVSAWRTFRAAYRTYRARRLAQEPI
                     PPTLQLQVRDARDRIIDLLGIGRTYPLRDLGVPDQIVVAPSSRAKIAIDHSQADVVYE
                     LHDRATGKPTLDDGAPIVVGGTGGPIEIVTPPIDIDASYRILAVKRAGAQTVDDRRAA
                     WLRTGPAPGKSIAIRVMEGVDTSLEASIQLPVLDARIETPQPTDARIGDWGIQADVQI
                     RASQEGVTYTLIANARDLSDPTRDQVLSVPAIGTSGTIVLRSAAITEDVDLRIRGQRT
                     TGIGGNPVTRTAVLDVVLPLRVRANPAVPAQLVPPVVDHGGAAQVKLDATQASAQYRV
                     HRGRVRDSDFVFDAAPAVPTIDVAGDGRTVRVRRPAKPSPWQDLSGFDPVGDATAGNG
                     GTLNLAIGVPGTDDTFLVIQATKQHPIGPLDSGFGTLPSSVQLDAALALLVRPDPAPG
                     LQLQVVMTGGATDGSLQVSGGQPGVFYELHLDGNPQPIGLPAYFHQHDDQSPQLNKGI
                     EQLRIEVDAVVERDRFDPTANPVTTPPLAPLIDTARLPAGSVLAVQARKAMSGLGAAL
                     TRRAVLDDLPVTAPRAVASGASADIAIEASRTGDRYWLIRAGQPVGDPKTGTGGKLVL
                     STGPVTQRSVFTVAATRPGDPGIAVERHFIVEIGIVVDVT"
BASE COUNT          347 a         1024 c         1056 g          380 t
ORIGIN      
        1 ccccgtcgac gcagcgcctg tgccagcgcg gcgcgcagcg cctgctcgag cctcgcgacg
       61 aagccgtcct cgaagccgtc ggccggcagg gtccccagat cgacctccag ccggtcgagc
      121 cgcaccagct cctcgccggc cgcgagctcc tcgcagaccc ggtcgagcac cggcaccacc
      181 cggcggccgt ggatctggcc gagccgctcg gacaccgcgc gggcgaccgg cacgctggcg
      241 ccgtgcagct cgacctcgag gtggttgatc cggtgctccg ccatgggctc acgtcacgtc
      301 gaccacgatg ccgatctcga cgatgaagtg gcgctccacc gcgatgcccg gatcgccggg
      361 ccgcgtcgcg gccaccgtga acacgctccg ctgggtcacg gggccggtcg acagcacgag
      421 cttcccgccg gtgccggtct tcgggtcgcc aaccggctgg ccggcccgga tcagccagta
      481 gcggtcgccg gtgcggctcg cctcgatcgc gatgtccgcc gacgcaccgc tggcgaccgc
      541 ccggggcgcg gtgaccggca ggtcgtcgag cacggcgcga cgcgtgagcg ccgccccgag
      601 cccgctcatc gccttgcgcg cctgcacggc cagcacgctg cccgccggca ggcgcgcggt
      661 gtcgatcagc ggggcgagcg gcggcgtggt caccgggttc gcggtcggat cgaaccgatc
      721 gcgctcgacc accgcgtcga cctcgatccg gagctgctcg atacccttgt tgagctgcgg
      781 gctctgatcg tcgtgctggt ggaagtacgc cggcaggccg atcggctgcg ggttgccgtc
      841 gagatggagc tcgtagaaca ccccgggctg accgccggac acctgcagcg agccgtccgt
      901 ggcgccgccg gtcatgacga cctggagctg cagccccggc gccggatccg gacggaccag
      961 cagcgccagg gccgcgtcga gctgcaccga ggacggcagc gtgccgaagc cgctgtccag
     1021 cggcccgatc gggtgctgct tggtcgcctg gatcaccagg aacgtgtcgt cggtgccggg
     1081 cacgccgatc gcgaggttca gcgtgccgcc gttgccggcg gtcgcgtcgc cgaccggatc
     1141 gaagccgctc aggtcctgcc acggcgatgg cttcgccggc cgccggaccc gcacggtgcg
     1201 gccatcgccc gcgacgtcga tcgtgggaac cgcgggcgcc gcgtcgaaca cgaagtcgct
     1261 gtcgcggacc cggccgcggt ggacgcggta ctgcgcggag gcctgcgtcg cgtcgagctt
     1321 gacctgagcc gcgccgccgt ggtcgaccac cggcgggacc agctgggccg gcaccgcggg
     1381 gttggcccgc acgcggagcg ggaggacgac gtcgagaacg gcggtgcggg tcaccgggtt
     1441 gccgccgatg ccggtcgtgc gctggccgcg gatccgcagg tcgacgtcct cggtgatcgc
     1501 cgcgctgcgc agcacgatcg tccccgacgt gccgatcgct ggcacggaca gcacctgatc
     1561 ccgggtgggg tcgctcaggt ccctggcgtt cgcgatcagc gtgtaggtga cgccctcctg
     1621 gctcgcgcgg atctggacgt cggcctggat gccccagtca ccgatccggg cgtcggtcgg
     1681 ctgcggcgtc tcgatccgcg cgtccagcac cggcagctgg atgctggcct ccagcgaggt
     1741 gtcgacgccc tccatcacgc ggatcgcgat gctcttgccg ggcgccggcc cggtgcgcag
     1801 ccaggccgcg cggcggtcat cgacggtctg tgcgccggcg cgcttcaccg cgaggatccg
     1861 gtagctggcg tcgatgtcga tcggcggcgt gacgatctcg atcgggccgc cggtgccgcc
     1921 gacgacgatc ggtgcgccgt cgtcgagggt cggcttgccg gtcgcgcggt cgtgcagctc
     1981 gtacaccacg tcggcctggc tgtggtcgat cgcgatcttc gcgcgcgacg acggcgcgac
     2041 gacgatctgg tcgggcaccc cgaggtcgcg cagcgggtag gtgcggccga tgccgagcag
     2101 gtcgatgatc cggtcccgcg catcgcgcac ctgcagctgc agggtgggcg ggatcggctc
     2161 ctgggcgagg cgtctggccc ggtaggtgcg gtaggcggcg cggaacgtcc gccaggcgga
     2221 cacgaacgcg gcccagtcgt cgaccccgtc ggtcttgccg agccactgca ggtcgaccga
     2281 caggtgggcc ggcgcctcgg cgaggacggt ctgctcgacg agctgctcga agctgccatg
     2341 gggatcgggc ggatcgagct ccgcgatcac gacgctgacg tgcagcgacc acgggtcggg
     2401 cccggacacg ccggcgagca gcgggacgtc cggctcgtcc tcgtcggcag caggcagctt
     2461 gtcctcgagc accggccgca gcagcacgtg ctccacgacg tacagccgct cgccgtcgcg
     2521 cagccccagc ttgtggcgca gccggtcctc gaagccgccg gcgtccgagg cccccgggct
     2581 caggtcgtag cccgagccgc ggccgcggct cagcgcgggg taggcgcgca ggaacgcgct
     2641 gcgatcggcg atcagcaggt cgtcgggatc ggcggcccgc ccctggggcg cgatccgcgc
     2701 gtggtcgccc agctcctcgg cgtagcgcgc caggaggtgg ccgaggaacc gcttgcgccg
     2761 ctcgcgcgga tcgccggcct cgacgtggcc gtcgagccag gcgcggt
//
LOCUS       DASVOR010002092         1737 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401322,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002092 DASVOR010000000
VERSION     DASVOR010002092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1737
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401322"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            257..339
                     /locus_tag="VF516_38870"
     tRNA            257..339
                     /locus_tag="VF516_38870"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:290..292,aa:Leu,seq:caa)
     gene            complement(377..1549)
                     /locus_tag="VF516_38875"
     CDS             complement(377..1549)
                     /locus_tag="VF516_38875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEX8113779.1"
                     /translation="MSRSPKAVPKDTYDLALLPGDGIGVEVMGHARQLLDVVARGTGA
                     AFTLDEIPCGGKFYLDHGSRDWPEDAEDRCRAADAILLGAVGWNGSGGAPVTMRDADG
                     REKMAGWSPVIGNRIRLDLYANIRPVRCLPGTRHGLSGRFREVWSPETVDMVIVRENT
                     EGLYSGIAERTAERATDLRVITRRASERVIRAAFELSRRRDRGAPGDGKRRVTCIVKH
                     NVLAGCRLFLDVFREVAGQFPEIEPDVAIVDSFAMFVLTQPERYDVCVTTNMFGDILT
                     DLASVLQGGMGMAVGCNVGDDHAMFEPIHGSAPPLVGKDRANPMAMLLATGEAMAWLG
                     RRFSDDRLVRAHRAIEAAVAGLVERGEPLTSDLGGSAPQSVVAEAVRDEVRRRLGA"
BASE COUNT          226 a          652 c          622 g          237 t
ORIGIN      
        1 cgcagcctcc ggccggcacg acgccgcctc cggccgccgg ctcgggctcg ggctcgaaca
       61 tgtaagccgg cgcgagcctg gctcgacaac gcggagcgcc ggtcgccgga ccggcgtttt
      121 gcgtttgccc cggtccgatc gcatcccggg tcacaccggg tcacattctg ggcgtgcgag
      181 gcgcttgacc gcgcccgccg cctcgtgtga ctgggaaacg agtggagaac gagtggacgg
      241 cccggccgtc ccgattgccg gagtggagaa atggtagact cgggggtctc aaaagcccct
      301 tcccgcgagg gagtgtcggt tcgagtccga cctccggcac ttgactgctc ctgactgcgc
      361 ctggccgcgc ctgacttcag gcgccgaggc ggcggcggac ctcgtcgcgc actgcctcgg
      421 cgaccaccga ctgcggcgcg ctgccgccca gatccgaggt cagcggctcg ccgcgctcga
      481 ccagccccgc caccgcggct tcgatcgcgc ggtgggcgcg gaccagccgg tcgtcggaga
      541 accgccggcc gagccacgcc atcgcctcgc cggtcgcgag cagcatcgcc atcgggttcg
      601 cgcggtcctt gccgaccagc ggcggcgccg agccgtggat cggctcgaac atcgcgtggt
      661 cgtcgccgac gttgcagccg accgccatgc ccatcccgcc ctgcagcacc gacgcgaggt
      721 cggtgaggat gtcgccgaac atgttggtgg tcacgcacac gtcgtagcgc tcgggctggg
      781 tcagcacgaa catcgcgaac gagtccacga tcgcgacgtc gggctcgatc tcggggaact
      841 gcccggcgac ctcgcggaac acgtcgagga acagccggca gccggcgagc acgttgtgct
      901 tgacgatgca ggtcacccgg cgcttgccgt cgcccggcgc gccccggtcg cggcggcgcg
      961 acagctcgaa cgccgcgcgg atcacgcgct cggacgcgcg ccgcgtgatc acgcgcaggt
     1021 cggtggcgcg ctcggcggtg cgctccgcga tcccggagta cagcccctcg gtgttctcgc
     1081 gcacgatcac catgtcgacg gtctcgggcg accacacctc gcggaaccgg cccgacagcc
     1141 cgtgccgcgt gccgggcagg cagcgcaccg gccggatgtt ggcgtagagg tcgagccgga
     1201 tccggttgcc gatcaccggc gaccaccccg ccatcttctc ccggccgtcg gcgtcgcgca
     1261 tcgtgaccgg cgcgccgccc gagccgttcc agccgaccgc gccgagcagg atcgcgtccg
     1321 ccgcgcggca gcggtcctcg gcgtcctcgg gccagtcgcg gctgccgtgg tccaggtaga
     1381 acttgccgcc gcacgggatc tcgtcgagcg tgaacgccgc gccggtgccg cgcgccacca
     1441 cgtcgagcag ctggcgggcg tgccccatca cctcgacgcc gatgccatca ccggggagca
     1501 gggcgagatc gtaggtgtcc ttggggacgg ccttgggcga gcgcgacatc gccggaacgt
     1561 agcatcgccc cggcccggcg atatactgcg gccgtgccgt cccgcgacga ctggcagcgc
     1621 cggctcggcc cgctctacgg tggcgcggtc gcgctcgtcc actcgacgcc gtggcgctgg
     1681 ccggccgagg tcggcgactc gccgcgcgcc gccggatggc tggtcgcgct cggtgcg
//
LOCUS       DASVOR010002093         1887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_80733,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002093 DASVOR010000000
VERSION     DASVOR010002093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80733"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..801)
                     /locus_tag="VF516_38880"
     CDS             complement(<1..801)
                     /locus_tag="VF516_38880"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113780.1"
                     /translation="MPAPAQKPLAPRSTGAKVGKYTLQSLLGQGAYGEVHLGVPSAGP
                     RVAVKILSATAARSADTVTRFQREADTARRLDHPNIIRILDVGSSRGRHYLIMELVHG
                     GSLRRLLERATAPPARVLAVLAEVAAALAYAHAQGVVHRDVKPENVLLTRAGRAKVAD
                     FGLARTVDEPSLTTQGRILGTAIYLSPEQARGQRATAASDVYAFGVMLYEAITGERPF
                     TADTQLGYLYQHAEVDPPRPVIRGRYPAALAGLALACLAKEPDDRPARA"
     gene            903..>1887
                     /locus_tag="VF516_38885"
     CDS             903..>1887
                     /locus_tag="VF516_38885"
                     /inference="COORDINATES: protein motif:HMM:NF028501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113781.1"
                     /translation="MSGRTDPERALAVIAERRLALELGTLDICFLVALHVRGEGAGLT
                     AFTEAQLEDVFAQAAAVAQPGADQLRRRATLAIQRLRDQRMLARVDGQGVVRTGEFAL
                     SRLATGIVRFFLEEDVLTRETLGVLTHGLQVALIGVREAAREAREPAAWQDRVVAPLQ
                     VTISELVAGIERRQRGLDLQQEDFQAEIRRLLEADWFGAIDRCQGLLESTSATLRELN
                     EVLLRDTAVLLGLLQDIEELAIAAGEPAAEAAAHRVIDQVDRIAAWGSARQRAWSEYF
                     QYVHRYLRDVVRLDPTRALLQRLRDQLAGAGPRFALAYAGAPPIRILRTVGG"
BASE COUNT          224 a          615 c          803 g          245 t
ORIGIN      
        1 ggccctcgcc gggcgatcgt cgggctcctt ggcgaggcac gcgagcgcca ggccggcgag
       61 cgccgcggga tagcggccgc ggatcaccgg gcgcggcggg tcgacctcgg cgtgctggta
      121 gaggtagccg agctgggtgt cggcggtgaa cggccgctcg ccggtgatcg cctcgtagag
      181 catgacgccg aacgcgtaga cgtcgctcgc ggcggtggcg cgctggccgc gggcctgctc
      241 gggcgacagg tagatggcgg tgccgaggat ccggccctgg gtggtgagcg agggctcatc
      301 gacggtgcgg gccagtccga agtcggcgac cttggcgcgg ccggcgcggg tcaggagcac
      361 gttctccggc ttgacgtcgc ggtggaccac gccctgtgcg tgggcgtagg cgagcgcggc
      421 ggcgacctcg gcgagcaccg cgagcacccg ggcgggcggc gcggtggcgc gctcgagcag
      481 gcggcgcagc gatccgccgt gcacgagctc catgatgagg tagtggcggc cgcgcgacga
      541 gccgacgtcg aggatgcgga tgatgttcgg gtggtcgagg cgccgcgcgg tgtcggcctc
      601 gcgctggaag cgggtgacgg tgtcggcgct gcgcgccgcg gtggcgctga ggatcttgac
      661 cgcgacccgg ggcccggcgg acggcacgcc gaggtggacc tcgccgtagg cgccctggcc
      721 gagcaaggac tgcagcgtgt acttgcccac cttcgcaccg gtcgaacgcg gggcgagggg
      781 tttctgcgcg ggggcgggca cgctgcgggt cctgggccga acgacaacca tagcaggccg
      841 gcggcgcgga tctgcgcgcg atcggtcgac cggccgcgca cgaggggcta tagacgaagg
      901 ccgtgtccgg acgcaccgac cccgagcgcg cgctggcggt gatcgccgag cggcggctgg
      961 cgctggagct cggcacgctc gacatctgct tcctggtggc gctgcacgtg cgcggcgagg
     1021 gcgcggggct gaccgcgttc accgaggcgc agctcgagga cgtgttcgcc caggcggcgg
     1081 cggtggcgca gcccggggcc gaccagctgc ggcggcgcgc gacgctggcg atccagcggc
     1141 tgcgcgatca gcggatgctg gcgcgggtcg acggccaggg cgtggtgcgc accggcgagt
     1201 tcgcgctgtc gcggctggcg accgggatcg tgcggttctt cctcgaggag gacgtgctga
     1261 cccgcgagac gctgggcgtc ctgacccacg ggctgcaggt cgcgctgatc ggggtgcgcg
     1321 aggcggcgcg cgaggcgcgg gagccggcgg cgtggcagga ccgcgtggtg gcgccgctgc
     1381 aggtgacgat cagcgagctg gtcgccggga tcgagcgccg gcagcgcggg ctcgatctgc
     1441 agcaggagga cttccaggcc gagatccgcc ggctgctgga ggccgactgg ttcggcgcga
     1501 tcgaccgctg ccaggggctc ttggagtcga cgagcgcgac gctgcgcgag ctcaacgagg
     1561 tgctgctgcg cgacacggcc gtgctgctcg ggctgctcca ggacatcgag gagctggcga
     1621 tcgccgcggg cgagccggcg gccgaggctg ccgcgcaccg ggtgatcgat caggtcgacc
     1681 ggatcgcggc gtggggctcg gcgcgccagc gcgcgtggtc ggagtacttc cagtacgtgc
     1741 atcgctacct gcgcgacgtg gtgcggctcg atccgacgcg cgcgctgctg cagcggctgc
     1801 gcgatcagct cgccggcgcc ggcccgcggt tcgcgctggc ctacgccggg gcgccgccga
     1861 tccggatcct gcgcacggtg ggggggc
//
LOCUS       DASVOR010002094         3142 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_197677,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002094 DASVOR010000000
VERSION     DASVOR010002094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197677"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..360)
                     /locus_tag="VF516_38890"
     CDS             complement(118..360)
                     /locus_tag="VF516_38890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113782.1"
                     /translation="MDKSDELRDRVEARKHELLAKYNELKADSRKEALEARTHLKAKL
                     DELEAHLKLGWAKVNDEVRTKLSQWLERKDQDNQHK"
     gene            564..911
                     /locus_tag="VF516_38895"
     CDS             564..911
                     /locus_tag="VF516_38895"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX8113783.1"
                     /translation="MARNTVRSPRSYDDIVRQTVVEPDTSVRPTRDQEQAAREGFRAL
                     DPDEQVLHDRVAQALATLGPEASRVTAEVSRELVTLRGQVADVAMLRRIEDAVAYVTG
                     VETIHNQVVVAAP"
     gene            908..1354
                     /locus_tag="VF516_38900"
     CDS             908..1354
                     /locus_tag="VF516_38900"
                     /inference="COORDINATES: protein motif:HMM:NF016045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity iron permease family protein"
                     /protein_id="HEX8113784.1"
                     /translation="MTVHGAFRKVAHATACAIGSPYAFAAAVMVVVVWAVCGPAFHYS
                     DTWQLVINTGTTIVTFLVVFMIQNTQNRDSRAIHLKLDELIRAVESARNRLVDVEDLP
                     DEELARLAREFQRLRRLADAHEATTAPPARARPGATATPPRSSDKP"
     gene            1596..1760
                     /locus_tag="VF516_38905"
     CDS             1596..1760
                     /locus_tag="VF516_38905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HEX8113785.1"
                     /translation="MFSWAIAFFVIALIAAVFGFFGLAASAAGIAKIIFVVALIMALI
                     SFVAGRRPVV"
     gene            1819..>3142
                     /locus_tag="VF516_38910"
     CDS             1819..>3142
                     /locus_tag="VF516_38910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8113786.1"
                     /translation="MNGTILIVDDDDDTAVLLRDSLRKRGYDVDAVNSAQQCLERLRT
                     DPADVVVTDVQMSGMSGIDLCVELRQRYPDLLPLVLTGQGGLDTAIAAIRAGAYDFIT
                     KPVKVDALAIAVQRAVEHLSLQREVMRLRRDAHRDLPIDGIAGGSPAIRETIEMIRRV
                     ADSDATVLVTGESGTGKELVARALHRLSPRANEPFVAVNCAAMPAPLLESELFGHVRG
                     AFTDAKRSRAGLFVQAGAGTIFLDEIGEMPIEMQVKLLRVLQERKVRPVGGDEEVPFE
                     ARVVTATNRDLETEVEEKRFREDLFYRINVVAIPVPPLRARAGDILLLAQYFLKRIAT
                     RTNKPVQGISGPAARMLMDYDWPGNVRELENCMERAVALCRLDEITVDDLPAKVQEHQ
                     SSKIVITTESPGELITLDEMERRYVRQVLNAVGGNKTHAARILGIDRRS"
BASE COUNT          478 a         1103 c         1080 g          481 t
ORIGIN      
        1 gcagccggcc aaaaaaaaaa ccgccggtag cctgggggag gctaccggcg ggacccgctt
       61 gcgcgggggt cagggcggct ctccggcgcg ggtgggacgc cagatgagcc gcaagcgtta
      121 cttgtgctgg ttgtcctggt ccttgcgctc cagccactgg ctcagcttgg tgcggacctc
      181 gtcgttgacc ttggcccagc cgagcttgag gtgggcctcc agctcgtcga gcttggcctt
      241 cagatgggtc cgagcctcga gggcttcctt gcgcgagtcg gccttcagct cgttgtactt
      301 cgcgagcagc tcgtgcttgc gagcttcgac ccggtcgcga agctcatcac tcttgtccat
      361 gccgggggtg tctgcacggt cgataccagg tgattaacct cgcggatcgc ctggacatcg
      421 gtccggagag gttccggatg taccgggtcg tgccgtgact gcggcgctcg gcgcgcgaat
      481 tcgcccgccg tggacgcacg ggggtgttcc ttggatccgg atccgccgcg cggctggcac
      541 tcgcgctgca caccgatcgg gacatggcgc gcaacacggt tcgaagcccc agaagctacg
      601 atgacatcgt ccgacaaacc gtcgtcgagc ccgacacgtc ggtccgcccg acgcgggacc
      661 aggagcaggc ggcccgcgag ggcttccgtg cgctcgatcc cgacgagcag gtgctccacg
      721 accgcgtggc ccaggcgctc gccacgctcg ggccggaggc atcgcgcgtg acggccgagg
      781 tcagccgcga gctcgtgacg ctgcgcggcc aggtcgccga cgtcgcgatg ctgcggcgga
      841 tcgaggacgc ggtcgcctac gtcaccggcg tcgagacgat ccacaaccag gtcgtggtcg
      901 cagcaccgtg acggtccacg gcgcctttcg caaggtggcc cacgccacgg cctgcgcgat
      961 cggctcgccg tacgccttcg ccgctgcggt catggtcgtg gtcgtgtggg cggtgtgcgg
     1021 gcccgcgttc cactactccg acacgtggca gctcgtcatc aacaccggca cgacgatcgt
     1081 gacgttcctc gtggtgttca tgatccagaa cacccagaac cgcgactccc gcgcgatcca
     1141 cctcaagctc gacgagctga tccgggcggt cgagtccgcg cgcaacaggc tggtcgacgt
     1201 cgaggatctg cccgacgagg agctcgcccg gctcgcgcgc gagttccagc gcctgcgccg
     1261 cctcgccgac gcgcacgagg ccaccaccgc accgcccgcc cgggcacgcc cgggcgcgac
     1321 ggcgaccccg ccgcgctcgt ccgacaagcc gtagcgggcg catgtgcgga gcaagcctac
     1381 ctggcgaccc gcaggccggc accgccggcg gggtgcggcc cggtgcccga gcggtctcgc
     1441 tcaggtgagc gtgcgcgccc gcccatcgag cgccagcgca cggctcgacc gcccgcgggc
     1501 atggcgattc gcggctctac ggggcccggc agttcggcat cagtctggca tgtacgctgc
     1561 accgacgagg ggcataaccc ataggagaga atccaatgtt ttcctgggcc attgcattct
     1621 tcgtcattgc gttgatcgcg gccgtgttcg gcttctttgg cctcgcagcc agcgccgcgg
     1681 gcattgcaaa gatcatcttt gtcgtggcgc tcatcatggc gctcatcagc ttcgtggccg
     1741 ggcgccgccc ggtcgtctga cgagcgcccc agagagagtg gcctcgactc accaggggca
     1801 gaaaggtatg acctcgctgt gaacggaacc atcctgatcg tcgatgatga tgacgacacc
     1861 gcggtgctgc tgcgcgacag cttgcgcaag cgcggctacg acgtggatgc cgtcaactcg
     1921 gcccagcaat gcctggagcg gcttaggacc gatccagcgg acgtcgttgt caccgacgtc
     1981 cagatgtccg ggatgtccgg gatcgatctg tgcgtcgagc tgcgccagcg ctacccggac
     2041 ctgcttccgc tcgtgctcac cggccagggc ggcctcgaca ccgcgatcgc cgcgatccgc
     2101 gccggcgcgt acgacttcat caccaagccg gtgaaggtcg acgcgctcgc gatcgccgtg
     2161 cagcgcgcgg tcgagcacct gtcgctgcag cgcgaggtca tgcggctgcg gcgcgacgcg
     2221 caccgggacc tgccgatcga cgggatcgcc ggcggcagcc ctgcgatccg cgagaccatc
     2281 gagatgatcc gccgggtcgc cgacagcgac gccacggtgc tggtcaccgg ggagtcgggc
     2341 accggcaagg agctggtcgc ccgcgcgctg caccggctgt cgccgcgcgc caacgagccg
     2401 ttcgtcgcgg tcaactgcgc cgcgatgccg gcgccgctgc tcgagagcga gctgttcggc
     2461 cacgtccgcg gcgcgttcac cgacgccaag cgatcgcgcg ccggcctgtt cgtccaggcc
     2521 ggggccggga cgatcttcct ggacgagatc ggcgagatgc cgatcgagat gcaggtcaag
     2581 ctgctgcgcg tgctccagga gcgcaaggtc cgtccggtcg gcggcgacga ggaggtgccg
     2641 ttcgaggccc gcgtggtgac cgcgaccaac cgcgacctcg agaccgaggt cgaggagaag
     2701 cggttccgcg aggacctgtt ctaccggatc aacgtcgtgg cgatcccggt gccgccgctg
     2761 cgcgcccgcg ccggcgacat cctgctgctc gcccagtact tcctcaagcg gatcgcgacc
     2821 cggaccaaca agccggtgca ggggatctcc ggccccgccg cgcgcatgct gatggactac
     2881 gactggccgg gcaacgtccg cgagctcgag aactgcatgg agcgcgcggt cgcgctgtgc
     2941 cggctcgacg agatcacggt cgacgacctg ccggccaagg tccaggagca ccagtcgagc
     3001 aagatcgtga tcaccaccga gtcccccggc gagctgatca cgctcgacga gatggagcgc
     3061 cgctacgtcc gccaggtgct caacgcggtc ggtggcaaca agacccacgc cgcgcgcatc
     3121 ctcggcatcg accggcgctc gc
//
LOCUS       DASVOR010002095         1920 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_168040,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002095 DASVOR010000000
VERSION     DASVOR010002095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1920
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168040"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VF516_38915"
     CDS             <1..357
                     /locus_tag="VF516_38915"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX8113787.1"
                     /translation="SGSPAHAVTARHASGVPPCAFFHEPRQFQLLANDVLPELARTRR
                     RVRLWSAGCGAGQEAWSLAMAVEEAALPPEVEVEILATDADRRALAIAAEAVYRDDQA
                     PGVTTWRRRARWCAVR"
     gene            complement(314..1168)
                     /locus_tag="VF516_38920"
     CDS             complement(314..1168)
                     /locus_tag="VF516_38920"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEX8113788.1"
                     /translation="MDRIELVKAIPLFESLDDDDLHALAHKLREVRVGAGERVFSQGD
                     EGDAMYVIESGAVDIVAGAGKHQVTLASLFVQQYFGELALLDGAPRSASAVASRPTQL
                     LALDRDDFVEFIKKRPDAALAIMHEVGERMRATNELMTRTVTRNVLEEEDRKLTVGER
                     IADTVAAFGGSWPFIFLFAGFMLVWMVWNSLFGPPLAFDPLPFMFLNLFLSTLAALQA
                     PIIMMSQNRQATKDKALAVNDFQINLKNEVAIDKILRNQSEVLLRLTALEQRLIAPRT
                     SGLDAAKS"
     gene            1388..>1920
                     /locus_tag="VF516_38925"
     CDS             1388..>1920
                     /locus_tag="VF516_38925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113789.1"
                     /translation="MTRHARILIVEDNRTVARDLEQRLGRLGFAIAGMTGLGEEAIVL
                     AEDERPDLVLMDIRLGAAMDGITAAEYIRARLGIPVVFLTMFADDATLRAASVSEAFG
                     YIVKPAQDRELRIVIEMALYKHAAERDRRRLEEQLRRSEKMEAIGQLAGGIAHDFNNV
                     LMAILSNCRALRRQLPA"
BASE COUNT          298 a          659 c          680 g          283 t
ORIGIN      
        1 agcgggagcc ctgcacacgc ggtgaccgcg cggcatgcta gcggggtgcc gccgtgcgcg
       61 ttcttccacg agccgaggca gttccagctg ctcgcgaacg atgtgctccc ggagctcgcg
      121 cgcacccgcc gccgggtccg gctatggtcg gcgggatgcg gcgcgggcca ggaggcgtgg
      181 agcctggcga tggccgtcga ggaggccgcg ctgccgcccg aggtcgaggt cgagatcctc
      241 gcgaccgatg ccgaccgccg ggcgctcgcg atcgccgccg aggcggttta tcgcgacgac
      301 caggcgccag gggtcacgac ttggcggcgt cgagcccgct ggtgcgcggt gcgatgagcc
      361 gctgctcgag cgcggtcaac cggagcagca cctcgctctg gttgcgcagg atcttgtcga
      421 tcgcgacctc gttcttgagg ttgatctgga agtcgttgac cgcgagcgcc ttgtccttcg
      481 tcgcctggcg gttctggctc atcatgatga tcggggcctg gagcgcggcg agcgtcgaca
      541 ggaacaggtt gaggaacatg aacggcagcg ggtcgaacgc cagcggcggg ccgaacagcg
      601 agttccacac catccacacc agcatgaagc cggcgaacag gaagatgaac ggccagctgc
      661 cgccgaacgc ggcgacggtg tcggcgatcc gctcgccgac ggtcagcttg cgatcctcct
      721 cctcgaggac gttgcgggtc accgtccggg tcatcagctc gttggtcgcg cgcatccgct
      781 cgccgacctc gtgcatgatc gcgagcgcgg cgtccggccg cttcttgatg aactcgacga
      841 agtcgtcgcg atcgagcgcc aggagctggg tcgggcggct cgccaccgcg ctggccgagc
      901 gcggcgcgcc gtcgagcagc gcgagctcgc cgaagtactg ctgcacgaac agcgacgcga
      961 gggtcacctg atgcttgccc gcgccggcga cgatgtcgac cgcgccgctc tcgatcacgt
     1021 acatcgcgtc gccctcgtcg ccttgcgaga acacgcgctc gccggccccg acgcgcacct
     1081 cgcggagctt gtgggcgagc gcgtggagat cgtcatcgtc gagggattcg aatagcggga
     1141 tcgctttcac cagctcgata cggtccatcg tgctgccacg gtaacccgag tcgcgcggtc
     1201 cgcggtcgcc cgcgccgccg atcacggccg attgctttcg cgggcaccac tacgaaatcg
     1261 gatcaggatg aacccgcccg ccggcggccc gctccgtcgg agagaccgcc ttaacggcgg
     1321 ggtgcagcga aaagatcaac ccaaacccat cggggcgcgc ggagctctgt cgtaccttgc
     1381 ggcccggatg acccggcatg cgcggatcct gatcgtcgag gacaaccgca cggtcgcgcg
     1441 cgatctggag cagcggctgg gccggctcgg cttcgcgatc gccggcatga ccgggctggg
     1501 agaagaggcg atcgtgctgg ccgaggacga gcggcccgat ctcgtgctga tggacatccg
     1561 gctcggcgcc gcgatggacg gcatcacggc cgccgagtac atccgcgccc ggctcggcat
     1621 cccggtggtg ttcctgacga tgttcgccga cgacgcgacg ctgcgcgccg cgagcgtcag
     1681 cgaggcgttc ggctacatcg tcaagcccgc gcaggaccgc gagctccgga tcgtgatcga
     1741 gatggcgctg tacaagcacg ccgccgagcg cgaccgccgc cgcctcgagg agcagctgcg
     1801 caggtccgag aagatggagg cgatcggcca gctcgccggc ggcatcgccc acgacttcaa
     1861 caacgtgctg atggcgatcc tcagcaactg ccgcgcgctg cgccgccagc tgcccgccga
//
LOCUS       DASVOR010002096         2048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_256595,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002096 DASVOR010000000
VERSION     DASVOR010002096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2048
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256595"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VF516_38930"
     CDS             complement(<1..863)
                     /locus_tag="VF516_38930"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8113790.1"
                     /translation="MASRLDPDVETTTLDNGLAVTTVALPHLHTAVCALFIKVGARFE
                     RPEDNGLSHFTEHMLFRGTERYPTSLALNTAVERLGSTLHAETGRDYTLFQLALDPAF
                     IAPAIEMLGELLARPRFSDIELERELILEEINEDYDEQGVEINADDIARGLVFEDHPL
                     GQRIIGPRANVERFTIDDVRRHFRTFYGARNANLCVAGPVEHGAVVAAAERALAQMPR
                     GKRAEVPAAPPLDPGPHMRHVPDAGSQTSVAVLFRAIPDLDARYVSLVALLRVLDDGM
                     STRLHYTLADQ"
     gene            988..1881
                     /locus_tag="VF516_38935"
     CDS             988..1881
                     /locus_tag="VF516_38935"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8113791.1"
                     /translation="MLLKKIVIAEDDDSIAHMVNMALGDAGFLCLRARDGEEALRLVK
                     AHDPDLLVLDVMMPRVDGLEVARRLKADVMWSRTPILMLTALAQVDDQVQGLEAGADA
                     YMSKPFDLRELGARVKALIRSARRERDRSPTTNLPGSRAIDEEIEGALKSGRPTACVH
                     IDVLNFDGYADAIGIARSEDTVRNLGQWLLDAARAASGGGAFVGHIGGSDFIAVLSPD
                     HVGAFVTSAESTFDAKRATFPGDAAQLALVLAIAPTDGLQLGDGLQLGAAADELGRRL
                     GKAMKAAKAAGKTNHVVWSAS"
BASE COUNT          295 a          679 c          793 g          281 t
ORIGIN      
        1 ctctggtcgg cgagcgtgta gtgcagccgc gtcgacatcc cgtcgtcgag cacgcgcagc
       61 agtgcgacca gcgagacata gcgcgcgtcg agatcgggga tcgcgcggaa cagcaccgcc
      121 accgaggtct gcgagccggc atcggggacg tggcgcatgt gcgggccggg gtcgagtggg
      181 ggcgcggcgg ggacctcggc gcgcttgccg cgcggcattt gcgcgagcgc acgttctgcg
      241 gcggcgacca ccgcgccgtg ctcgaccggg ccggcgacgc acaggttggc gttgcgcgcg
      301 ccgtagaagg tccggaagtg gcggcgcacg tcgtcgatcg tgaaccgctc gacgttggcc
      361 cgcgggccga tgatgcgctg gccgagcggg tggtcctcga acaccaggcc gcgcgcgatg
      421 tcgtcggcgt tgatctcgac gccctgctcg tcgtagtcct cgttgatctc ctcgaggatc
      481 agctcgcgct cgagctcgat gtcggagaac cgcggccggg cgagcagctc gccgagcatc
      541 tcgatcgccg gggcgatgaa cgcgggatcg agcgcgagct ggaacagcgt gtaatcgcgg
      601 ccggtctcgg cgtgcagggt cgagcccagc cgctcgaccg cggtgttgag cgcgagcgag
      661 gtcgggtagc gctcggtgcc gcggaacagc atgtgctcgg tgaagtgcga caggccgttg
      721 tcctccggcc gctcgaaccg ggcgccgacc ttgatgaaca gcgcacacac cgcggtgtgg
      781 aggtggggca gggcgaccgt ggtgaccgcg aggccgttgt cgagcgtggt cgtctcgacg
      841 tcggggtcca ggcgggaggc cacgcggatg cttaccacag cgcgggcgcc ggcagacgca
      901 aagccgtgcg cggtccgtac gccggcttgc cacccggact tggcgttgag gcgccggggg
      961 atcggtggta tttatcgaag cgatccgatg ctcctcaaga agatcgtgat cgccgaggac
     1021 gacgactcca tcgcgcacat ggtgaacatg gcgctcgggg atgcgggatt tttgtgcctg
     1081 cgggcgcgcg atggtgagga ggcgctgagg ctggtcaagg cccatgaccc ggacctgctc
     1141 gtgctcgacg tcatgatgcc gcgggtcgac gggctggagg tcgcgcgccg gctcaaggcc
     1201 gacgtgatgt ggtcgcgcac gccgatcctg atgctgaccg cgctcgccca ggtcgacgac
     1261 caggtccagg gcctggaggc gggggcggac gcctacatgt ccaagccgtt cgacctccgg
     1321 gagctcggcg cgcgggtcaa ggcgctgatc cgctcggcgc ggcgggagcg cgatcgcagc
     1381 ccgacgacca acctgcccgg gtcgcgcgcg atcgacgagg agatcgaggg cgcgctcaag
     1441 agcggccggc cgaccgcgtg cgtccacatc gacgtgctga acttcgacgg ctacgccgac
     1501 gcgatcggca tcgcgcgcag cgaggacacc gtgcgcaacc tcgggcagtg gctgctcgac
     1561 gcggcgcgcg cggcgtcggg cgggggtgcg ttcgtcggcc acatcggcgg ctcggacttc
     1621 atcgccgtgc tgtcccccga ccacgtcggc gcgttcgtca cgagcgccga gtcgacgttc
     1681 gacgccaagc gcgcgacgtt ccccggcgac gccgcgcagc tcgccctggt gctcgcgatc
     1741 gcccccaccg atggcctcca gctcggggat ggcctccagc tcggggccgc cgccgacgag
     1801 ctcggccgcc ggctcggcaa ggcgatgaag gccgccaagg ccgccggcaa gaccaaccac
     1861 gtggtgtggt cggctagctg aacgtggcgg gggccaggcc gcccaacggg tcgggaccgc
     1921 cgccagaccc ggcgccagac ccggcgccgg gacatgagcc gggacatgag ccgggacgtg
     1981 agggtgagct cgacacggtg gtcggcgaga ctgcgcgccg gggcccgcgc cgggtgcgga
     2041 ccggcgac
//
LOCUS       DASVOR010002097         1525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_168101,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002097 DASVOR010000000
VERSION     DASVOR010002097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168101"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..577)
                     /locus_tag="VF516_38940"
     CDS             complement(281..577)
                     /locus_tag="VF516_38940"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX8113792.1"
                     /translation="MATMPATAYTEAIAMALPRKLVTELLELPDEDRGTLAVLLWRSL
                     EPDDGKEVTGKEWEAAWAAVIERRMREVAEGKVELVDGDQVDAEIQALIDAPVP"
     gene            1010..1216
                     /locus_tag="VF516_38945"
     CDS             1010..1216
                     /locus_tag="VF516_38945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEX8113793.1"
                     /translation="MKKLEVVARGGSWFAPIESEEAMLLVAQCLREGWLVIRDYNAAL
                     RWLRRAARVGSATVAGLLRDLEAE"
     gene            complement(1190..>1525)
                     /locus_tag="VF516_38950"
     CDS             complement(1190..>1525)
                     /locus_tag="VF516_38950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113794.1"
                     /translation="SRRHDRQFLRIRSAVYNVGTRDRRTLVGAARRPTDLGDQLGPEL
                     VALAGDVAQAQALDAEHRRDRGEAEELEPHRVTSSAAIAGAGAPWGTPATSTRPGGSV
                     GSLCFEVAQ"
BASE COUNT          218 a          551 c          519 g          237 t
ORIGIN      
        1 actgtcgacc gtcatggcgg cgcggagcca aaaccagttc gccagctgct ccggcgtcgc
       61 cgggtgcgac accacataag cgaccgccac ctgcgagatg tcgaacgcaa cgacctcgta
      121 cccggcgtgc gcgatcacgc aagcggccgt tcccggatgc tgcgcagccc cgcgttcacc
      181 gcgtcgcgca ggcgcctcgc cgcgtcctgc gatcgctgtc gataccaggc ggccgctgct
      241 cgatactcgg cgatggcttc aggatgatac cggtaccgga tcacggaacg ggcgcatcga
      301 tcagagcctg gatctcggcg tccacctggt caccgtcgac cagctccacc ttcccctccg
      361 caacttcgcg catgcggcgc tcgattaccg ccgcccaggc cgcctcccac tccttgccgg
      421 tgacctcctt gccatcgtcc ggctcgaggc tacgccacag gaggaccgcc agcgtcccgc
      481 ggtcctcgtc gggaagctca agcagctcgg tgaccagctt gcgggggagc gccatggcga
      541 tggcctcagt gtacgcggtg gcgggcatcg tcgccatggg cctaccgggc ccgccgtcgc
      601 cgttcggtca agggatccgc cgccggacgc gcttgcagcg tccggacctc caccgccagc
      661 gccctggcct gctcgtggtc gccggccagg accgcctccg cgagcgctgt agcaaggctt
      721 gctacagtgc ctgtccgcga aggtccgcga gcgtcctgct cggatcccgg atctccgtcg
      781 gagaccggcg gatcctggcg gaccgtcgat gccgcggaag ggctttcaag tcccattggg
      841 gtcactactt ttctccagcg cttctctccc cttcgctcgg tggtagcaag actggtagca
      901 agcggcagca cctcgccgcc gaggatgccc acgcggagct tgcccacctc gcggcagatc
      961 gtcgcccacg tgaaccgggg ccgggacgcg gcggaacgtc cccgccgcaa tgaaaaagct
     1021 cgaggtggtc gcgcgcggcg gtagttggtt tgctccgatc gaaagcgaag aggcgatgct
     1081 gctcgtggcc cagtgcctcc gcgagggttg gctcgtgatc cgcgactaca acgcagccct
     1141 gcgctggctg cgacgggccg cgcgggtggg aagcgcgaca gtcgccggcc tactgcgcga
     1201 cctcgaagca gagtgatccg accgatccgc cgggccgggt cgaggtcgcc ggcgtgcccc
     1261 agggcgcccc tgcccccgcg atcgccgccg agctggtgac ccggtgcggt tcgagctcct
     1321 cggcctcgcc acggtcccgc cggtgctcgg cgtcgagggc ctgggcctgg gcgacgtcgc
     1381 cggcgagcgc cacgagctcc gggccgagct ggtcgccgag gtcggtcggt cggcgcgcag
     1441 cgccgacgag ggtccgtcgg tcacgtgtac ccacgttgta gacggcagag cggatccgta
     1501 gaaattgacg gtcgtgtcga cggga
//
LOCUS       DASVOR010002098         1585 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_227017,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002098 DASVOR010000000
VERSION     DASVOR010002098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1585
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227017"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            262..1152
                     /locus_tag="VF516_38955"
     CDS             262..1152
                     /locus_tag="VF516_38955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX8113795.1"
                     /translation="MQVHLIDGTYELFRAWFGAPQAQVNGREVGAVRSLVRSLSALLS
                     RSTQAPDGGRSRVPMTHVGVAFDHVIESFRNDLFDGYKTGEGLPEGLVSQFELAERAS
                     AALGLVVWPMVEFEADDAIATAAHRLAGDPAVERVLITAVDKDLCQCVIGDRVVCWDR
                     FKDQILDEPAVIAKHGVAPASIPDLLALVGDTADGIPGIPGWGKSSAARMLTVHHHIP
                     DIPDDPTAWTAPPRGADRLARALREQRTEAALYRTLATLRTDCPIVCDAAALAWRGVD
                     RPALDAICAELGVEPASIRL"
     gene            1253..>1585
                     /locus_tag="VF516_38960"
     CDS             1253..>1585
                     /locus_tag="VF516_38960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113796.1"
                     /translation="MSGFPEVFRVTYARYGELRDDLAQQMARGGLLVKVQDAPGLDFD
                     SAVALELVLPDGTTLQGTGKVLQVFAGVGFAMTVQPQLVEEVTRFAGRPEVAASGTAR
                     HERVERPML"
BASE COUNT          210 a          563 c          586 g          226 t
ORIGIN      
        1 ggccagcggc gacaacgcga ctcgccgtgg gcgaccgcga gcggatgggg tcgggacgcc
       61 gtccgcgcag gcgccggtgg tgatccggcg ggcgtgtcgc gtgtgcgcgt tctcctgggc
      121 gccatgcctt gccgaggctg aggccgagat cgataggtga cgcggatcca tggagctcct
      181 cggtcgagac cggcgcgcct gcgcacggcc ggcgacgagc aggatacgca cgccccggct
      241 tccccgtgta ggcttgctgg cgtgcaggtc cacctgatcg acgggacata cgagctgttc
      301 cgggcctggt tcggcgcccc gcaggcccag gtgaacggcc gcgaggtcgg cgccgtgcgc
      361 agcctggtcc gcagcctgtc ggcgctgctg tcgcgatcga cgcaggcgcc ggacggcggg
      421 cgaagccggg tgccgatgac gcatgtcggg gtcgcattcg atcacgtgat cgagtcgttc
      481 cgcaatgacc tgttcgacgg ttacaagacc ggcgagggcc tgcccgaggg cctggtcagc
      541 cagttcgagc tcgccgagcg cgcgtcggcc gccctgggcc tcgtggtgtg gccgatggtc
      601 gagttcgagg ccgacgacgc gatcgcgacc gccgcgcacc ggctcgccgg cgatcccgcg
      661 gtcgagcgcg tcctgatcac cgcggtcgac aaggacctgt gccagtgcgt gatcggcgac
      721 cgcgtggtgt gctgggaccg cttcaaggac cagatcctcg acgagcccgc ggtgatcgcc
      781 aagcacggcg tcgcacccgc gtcgatcccc gacctgctcg cgctggtcgg cgacaccgcc
      841 gacggcatcc ccggcatccc cggctggggc aagagctcgg ccgcgcggat gctcacggtc
      901 catcaccaca tccccgacat ccccgacgat cccacggcgt ggaccgcgcc gccgcgcggc
      961 gccgatcgcc tggcgcgcgc gctgcgcgag cagcgcaccg aggccgccct gtatcgcacg
     1021 ctcgccacgc tccggaccga ctgcccgatc gtctgcgacg ccgcggcgct ggcctggcgc
     1081 ggggtggatc gccccgcgct cgacgcgatc tgcgccgagc tcggcgtcga gcctgcgtcg
     1141 atccggctgt gatggcccgg ctgtgatggc ccggctgtga tgccggccat gcctgcggtg
     1201 aggcatccgg aagggctgaa aaaaatctcc ggactggagt acgatgggca ggatgtccgg
     1261 attcccggag gtcttccgag tcacgtacgc gcgctacggc gagctgcgcg acgacctcgc
     1321 gcagcagatg gcccgcggcg gcctgctggt gaaggtgcag gatgcgccgg gcctcgactt
     1381 cgacagcgcg gtcgcgctcg agctggtgct gcccgatggc acgacgctgc agggcaccgg
     1441 caaggtgctg caggtgttcg ccggcgtcgg cttcgcgatg accgtgcagc cccagctggt
     1501 cgaggaggtc acccggttcg ccggccgccc cgaggtcgcc gccagcggca ccgcgcgcca
     1561 cgagcgcgtc gagcgcccca tgctg
//
LOCUS       DASVOR010002099         2819 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_430758,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002099 DASVOR010000000
VERSION     DASVOR010002099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2819
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430758"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2096
                     /locus_tag="VF516_38965"
     CDS             <1..2096
                     /locus_tag="VF516_38965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113797.1"
                     /translation="TASQSAAARQHAAAQDAAAEQATARAPDVAQPGPASELPRTGKR
                     ASADGGILGWVGDRIHDAENWAEDKIEGAVMSVAPGLATLIKEGPGGLLKNAIQPAIQ
                     SWVGTVTGGVDIGEIAGQLKQSFSSAFAVLQGAKTGDARCCETLVNGINAIREVAHAF
                     MNNPVMDAIKSVFTKVSDIVNTVTKLVIGPAFEVLKTLVGGAWDAIGETIGYLVGMIA
                     FQALLDYLSAGTWTGAMAVISGIAKFLNWPMAFLGEAMGLLKKLGGFILDGLKSLGKM
                     AADAAAGALHEVLGAFREIGTKLGEFADELLSKFGGKTAENDAAHTLENDAARTAEND
                     AARDAGAAKDGKPGDGDGKPGDGAADSAATKEAQKAAELPQAIAISRSIATAEDVGGV
                     PGPAIAVSLDALKARYSWIKAYEAVPNAAGFEIFLIASKFSIIQAHNRMAQLPMDVQI
                     QFSGLSDEAITKFAALDDAALTRFATLDETALIKFGELDEAALAKFGQLSEAELAKFA
                     KLDRTALENFGALRELNMLQAYGQLSDEALAKFAKLDRGTLEKFAGWAHDNPRPILQN
                     IALQNDETIDLLSKLKVQPESKAINASAPFETRQIRAPGMTYQIDRGTISHVLERHHP
                     DFWIGEVKQIQSFLPRSLTPADLSDVIMDVFNQNRDKILARGAGTFQVEGVSHGFRWT
                     VGFKNGRIGQLYPIF"
     gene            2114..2596
                     /locus_tag="VF516_38970"
     CDS             2114..2596
                     /locus_tag="VF516_38970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113798.1"
                     /translation="MIETNTFVLANGKYQPLATVQGPLRDPDYIDGILELSIDGHAII
                     TRNAWDLVDQLWAYIVDGAVELTQGRSFRTRFPDQPLELLFTLDASADTVTVELRHPN
                     IDRPAKASVGRRELIDAIARGAEPVFARLIELVPDNRRGYEPTLEQIELLKHPERWAE
                     "
     gene            2593..>2819
                     /locus_tag="VF516_38975"
     CDS             2593..>2819
                     /locus_tag="VF516_38975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113799.1"
                     /translation="MSEHVSHPLVSEAALPGRMISPPPVSAMPQAPRERPGERGPADA
                     ANRVERDRDEGQDRGDAGDRDRGGRERPRDAG"
BASE COUNT          517 a          956 c          951 g          395 t
ORIGIN      
        1 cgaccgcatc acagagcgcg gccgcacgac agcacgccgc cgcacaggac gcagcggccg
       61 agcaggccac tgcgcgggcc cccgacgtcg cgcagcccgg accggcgagc gagctgccgc
      121 gcaccggcaa gcgcgcctct gccgacggcg gcatcctcgg ctgggtcggc gatcggattc
      181 acgacgccga gaactgggcc gaggacaaga tcgaaggcgc ggtgatgagc gtcgcgcccg
      241 gccttgcgac cctgatcaag gaaggaccgg gcggcctgct caagaacgcc atccagcccg
      301 cgatccagag ctgggtcggc acggtcacgg gcggagtcga catcggcgag atcgccggcc
      361 agctcaagca gtcgttctcg tcggcgttcg ccgtcctgca gggtgcgaag accggcgatg
      421 cccggtgctg cgagacgctg gtcaacggga tcaacgcgat ccgcgaggtc gcgcacgcgt
      481 tcatgaacaa cccggtgatg gacgcgatca agagcgtctt caccaaggtc tccgacatcg
      541 tcaacaccgt caccaagctg gtgatcggcc ccgcgttcga ggtgctcaag accctcgtcg
      601 gtggggcgtg ggatgcgatc ggcgaaacga tcggctacct ggtcgggatg atcgcgttcc
      661 aggcgctgct cgactacctg tcggcgggca cctggaccgg ggccatggcg gtgatctcgg
      721 ggatcgccaa gttcctgaac tggccgatgg cgttcctcgg cgaggcgatg gggctgctga
      781 agaagctcgg cggtttcatc ctggacggtc tcaagagcct gggcaagatg gccgccgacg
      841 cggccgccgg agcactccac gaggtgctcg gcgcgttccg cgagatcggc accaagctcg
      901 gcgagttcgc cgacgagctg ctgagcaagt tcggcggcaa gaccgccgag aacgacgcgg
      961 cccacacgct cgagaacgac gccgcacgca ccgccgagaa cgacgccgca cgcgatgccg
     1021 gcgccgcgaa ggacggtaag cccggcgatg gcgacggcaa gcccggcgac ggcgcggccg
     1081 actccgcggc cacgaaggaa gcgcagaagg cggcagagct tccccaggcg atcgcgatct
     1141 cgcggtcgat cgcgaccgct gaggatgtcg gcggagtgcc aggacccgca atcgcggtgt
     1201 cgctcgacgc gctcaaggcg cgctactcgt ggatcaaggc gtacgaggcg gtgccaaacg
     1261 cggccggctt cgagatcttc ctgatcgcaa gcaagttctc gatcatccag gcgcacaatc
     1321 gcatggccca gctgccaatg gacgtgcaga tccagttctc ggggctgagc gacgaagcga
     1381 tcaccaagtt cgccgcgctg gacgacgccg cgctgacccg gttcgcgact ctcgacgaga
     1441 ccgcgctgat caagttcggc gagctcgacg aggcggcgct cgccaagttc ggccagctca
     1501 gcgaggccga gctcgccaag ttcgccaagc tcgaccgcac cgccctggag aacttcggtg
     1561 cgctgcgcga gctcaacatg ctgcaggctt acggtcagct cagcgacgag gcgctcgcca
     1621 agttcgccaa gctcgaccgg gggacgctgg agaagttcgc cggatgggcg cacgacaatc
     1681 ctaggccgat cttgcagaac atcgcgctgc agaacgacga gaccatcgac ctgctgtcca
     1741 agctcaaggt ccagcccgag tcgaaggcga tcaacgcatc cgcgccgttc gagacccgcc
     1801 agatccgcgc ccccggcatg acgtaccaga ttgaccgtgg cacgatctcg cacgtgctgg
     1861 agcgccacca tcctgacttc tggatcggcg aggtcaagca gattcaatcg tttctgcccc
     1921 ggagcttgac cccggccgac ctctccgacg tgatcatgga tgtgttcaac cagaaccgcg
     1981 acaagatcct cgcgcgcggt gccgggacct tccaggtcga gggcgtgtcg cacggcttcc
     2041 gctggacggt cggcttcaag aacggccgga tcggccagct ttacccaata ttctgactcg
     2101 gtttattaga tacatgatcg agaccaacac cttcgtgctc gcgaacggca agtaccagcc
     2161 gctcgcgacg gtgcagggcc cgctgcgcga tccggactac atcgatggca tcctggagct
     2221 ctcgatcgac ggccacgcga tcatcacgcg gaacgcatgg gatctcgtcg accagctctg
     2281 ggcctacatc gtcgacggcg ccgtggagct cacgcagggc cgcagcttca ggacgcgatt
     2341 tcccgatcag ccgctggagc tcttgttcac gctcgatgcc agcgcggaca cggtgacggt
     2401 cgagctccgc catcccaaca tcgaccgtcc ggcgaaggcc agcgtcggcc ggcgtgagct
     2461 gatcgacgcg atcgcacgcg gcgccgagcc ggtgttcgcg cggctgatcg agctcgtccc
     2521 cgacaaccgc cgcggctatg agccaacgct cgagcaaatc gagctgttga agcaccccga
     2581 acgctgggcg gaatgagcga gcatgtcagc catccgctgg tgtcggaggc cgcgctgccg
     2641 ggccggatga tctccccgcc gccggtctct gcgatgccac aggcaccgcg cgagcggccc
     2701 ggcgagcgcg gcccggcgga cgcggcgaat cgcgtagagc gcgatcgcga cgagggacag
     2761 gaccgcggcg acgcgggcga tcgggaccgc ggcggacgcg agcggccccg cgacgccgg
//
LOCUS       DASVOR010002100         2256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_168137,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002100 DASVOR010000000
VERSION     DASVOR010002100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2256
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168137"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="VF516_38980"
     CDS             complement(<1..249)
                     /locus_tag="VF516_38980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113800.1"
                     /translation="MTSSLFEPPALCGPLRRPRQMLAEQRYDGHKSIHDDATAGGLGL
                     RAGPIEGPTHFSQFDPLLHHLHGQAWFETGGLSAHYQNM"
     gene            complement(433..780)
                     /locus_tag="VF516_38985"
     CDS             complement(433..780)
                     /locus_tag="VF516_38985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113801.1"
                     /translation="MTALFISYVCDYCDGLAPINWLRGYVVFRGEEDFERPIYVFPSQ
                     SDAAIYRSRNGWQDYPIREVRFEHPISWTKASGSLTGVTLAARPFTLHRDHRFEPLPY
                     HCGLKPLTDSLAA"
     gene            complement(1116..1835)
                     /locus_tag="VF516_38990"
     CDS             complement(1116..1835)
                     /locus_tag="VF516_38990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113802.1"
                     /translation="MARAKANTRWTVLPHTPIERLSERLWRIEGALPNMPLRRVMAIA
                     RRSDGGLVVHNAIAVDDAALAAIEAWGPVAAIFVPSGYHRLDAVVFHDRYPAARVICP
                     RGARAKVEQVVPVTGNYEDEPADPAVALETLDGTAANEGVMIVRDGDGATLVFNDIVF
                     NMPHLRGVSGFVLRWITASSGGPRVSRIARWFIAKDKPALRGHLERLAATPQLRRIVV
                     SHHEVIDRDPARVLREVAATL"
     gene            complement(1885..>2256)
                     /locus_tag="VF516_38995"
     CDS             complement(1885..>2256)
                     /locus_tag="VF516_38995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113803.1"
                     /translation="HRGKHPELRDRVIVPDVLLQSHSASLGVMFYTGTQFPPEYRQQA
                     FAAEHGSWNRARRTGYKVIWAPVRDGVPTGEYVDFMTGMVTPDGNVWGRPVAVTQTRD
                     GALVVSDDAGSCLWRIRYRGK"
BASE COUNT          324 a          781 c          832 g          319 t
ORIGIN      
        1 catgttctgg tagtgcgcgc tgagaccgcc ggtctcgaac caggcctggc cgtggaggtg
       61 atgcagcagc ggatcgaact ggctgaagtg ggtcggcccc tcgatcggcc cggcgcgcag
      121 accgaggccc ccggccgtcg cgtcatcgtg gatcgacttg tggccgtcgt agcgctgctc
      181 ggccagcatc tggcgcgggc gccgcagcgg cccgcacagc gcgggcggtt cgaacagcga
      241 ggaggtcatg agggtcctgc accgaggcgg gagccggtcc cgccgggagc gggcgcatca
      301 tcgtgcaacg agcttgcgct gtgcaagccg acgaatggct tgcttgctca cgctacaggg
      361 cgaatagcac catgtcgcaa catcgattgt ctccggtccg gcctcgcgga cgttcccgtc
      421 caccgggtcg tcctaggccg cgagcgagtc ggtcagcggc ttgaggccgc agtggtacgg
      481 cagcggctcg aagcgatggt cgcggtggag cgtgaacggc cgggccgcga gcgtcacgcc
      541 ggtcaggctg ccgctggcct tggtccagga gatcgggtgc tcgaagcgca cctcgcggat
      601 cgggtagtcc tgccagccgt tgcggctgcg atagatcgcg gcgtcgctct gcgacgggaa
      661 cacgtagatc ggccgctcga agtcctcttc gccgcggaac accacgtagc cgcgcagcca
      721 gttgatcggt gccagcccgt cgcagtagtc gcagacgtag gaaatgaaca gggcggtcat
      781 ccttcgcttg cacgcggggc actgcatcgg cgagaggcta gaagcccggc gccccgtcga
      841 caagcaacca gcgctaaccg gcgcgtgagg gacagcgggc accagcgcgc cggcgcacgc
      901 gccccgacga catgcgcggc tggaccgccc ggcgcgacgg cgctgaagga tcctcgcaac
      961 tgcgaggtcg ccggacctac gtggctgccg caaggtgccg gtgtttcgat gcgccgcggc
     1021 gacgcagtga ccgctccggc ggatgctgcg atgcgtccag cgtgaagcgg gcgtgatcac
     1081 ggcgcctggc tgtcgacggg ccgcgcgcgc tgcggtcaga gcgttgcggc gacctcgcgc
     1141 agcacgcgcg ccgggtcgcg atcgatgacc tcgtggtgcg agaccacgat ccggcgcagc
     1201 tgcggcgtgg ccgcgaggcg ctcgaggtga ccgcgcagcg cgggcttgtc cttggcgatg
     1261 aaccagcgcg cgatccgcga cacgcgcggg ccgccgctcg acgccgtgat ccagcgcagc
     1321 acgaagccgc tgacgccgcg caggtgcggc atgttgaaca cgatgtcgtt gaacaccagc
     1381 gtcgcgccgt cgccgtcgcg cacgatcatc acgccctcgt tcgccgcggt gccgtcgagc
     1441 gtctccagcg cgaccgcggg gtcggcgggc tcgtcctcgt agttgccggt caccggcacg
     1501 acctgctcta ccttggcgcg cgccccgcgc gggcagatca cgcgggcggc gggatagcgg
     1561 tcgtggaaca cgacggcgtc gagccggtga tagccgctcg ggacgaagat cgcggcgacc
     1621 ggcccccagg cctcgatcgc ggcgagcgcc gcgtcgtcga ccgcgatcgc gttgtgcacg
     1681 accaggccgc cgtcgctgcg ccgggcgatc gccatcacgc gccgcagcgg catgttgggg
     1741 agcgcgccct cgatccgcca caggcgctcc gacagccgct cgatcggcgt gtgcggcaac
     1801 acggtccacc gggtgttcgc cttcgctctc gccatgcgcg cagggtagcg ccgtcgcccg
     1861 cgcggcggcc aacgggcggt gcggctactt gccgcgatag cggatccgcc acaggcagct
     1921 gccggcgtcg tcactgacga ccagcgcgcc gtcgcgcgtc tgggtgaccg cgaccggccg
     1981 gccccacacg ttgccgtcgg gagtgaccat gccggtcatg aagtcgacgt actcgccggt
     2041 cgggacgccg tcgcgcaccg gcgcccagat caccttgtag ccggtgcggc gcgcgcggtt
     2101 ccacgagccg tgctccgcgg cgaatgcctg ctgccggtac tccggcggga actgcgtgcc
     2161 ggtgtagaac atcacgccga gcgacgccga gtgcgactgg agcagcacgt cggggacgat
     2221 cacgcgatcg cggagctcgg ggtgcttgcc gcggtg
//
LOCUS       DASVOR010002101         1563 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_460459,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002101 DASVOR010000000
VERSION     DASVOR010002101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460459"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VF516_39000"
     CDS             <1..1448
                     /locus_tag="VF516_39000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEX8113804.1"
                     /translation="EPLRAAARETAAARGQPGWRFANTRSAMEPFLICSTRRDLRERG
                     WRMWVSRGEHAGEHDNRPLIPEILALRAEKARLLGYPSHAHWTIDDNMARTPDAAMAL
                     LRKVWKTAVARAREEIADMQAVADAEHAGITIAPWDYRLYAEKVRKAKYDLDDNEVKP
                     YLQLDRMRDAMMWVAGQLYGLAFHRLDGVPVCHPDVTVYEVTRDGRQVGLWYFDPYAR
                     DGKLSGAWMSEYRTQEEITEPVSPIVSNNANFMKASGAVLISWDDARTMFHEFGHALH
                     GLCSAVHYRTLAGTSVKRDFVEFPSQLHERWLATPEVLGRFARHYQTGAPIPDELVAK
                     IERARHFNQGFATVEYLASAIYDLEIHTAPADPQIDPIAFERRVMAEIGCPPEIVMRH
                     RPTQFAHIFASDGYSAGYYAYLWADTLVADAAEAFTEAGSFYDPAIARRLFDSVMRVG
                     NSVPPHQAFHAFRGRDVDTDALMRDRGFPVV"
BASE COUNT          231 a          572 c          543 g          217 t
ORIGIN      
        1 ccgagccgct gcgcgccgcc gcgcgcgaga ccgccgccgc ccgcggccag cccggctggc
       61 ggttcgcgaa cacgcgctcg gcgatggagc cgttcctgat ctgctcgacg cggcgcgacc
      121 tgcgcgagcg cggctggcgg atgtgggtca gccgcggcga gcacgcgggc gagcacgaca
      181 accgcccgct gatccccgag atcctcgcgc tgcgcgccga gaaggcccgc ctgctcggct
      241 acccgagcca cgcgcactgg acgatcgacg acaacatggc gcgcacgccc gacgcggcga
      301 tggcgctgct gcgcaaggtg tggaagaccg cggtcgcccg cgcgcgcgag gagatcgccg
      361 acatgcaggc cgtcgccgac gccgagcacg cgggcatcac gatcgcgccg tgggactacc
      421 ggctctacgc cgagaaggtg cgcaaggcca agtacgacct cgacgacaac gaggtcaagc
      481 cgtacctcca gctcgaccgg atgcgcgacg ccatgatgtg ggtcgccggc cagctctacg
      541 gcctggcgtt tcaccggctc gacggcgtgc cggtctgcca ccccgacgtc acggtctacg
      601 aggtgacgcg cgacggccgc caggtcggcc tgtggtactt cgacccgtac gcgcgcgacg
      661 gcaagctgtc gggcgcgtgg atgagcgagt accggacgca ggaagagatc accgagccgg
      721 tctcgccgat cgtctcgaac aacgccaact tcatgaaggc gagcggggcg gtgctgatct
      781 cgtgggacga cgcgcggacg atgttccacg agttcggcca cgcgctgcac gggctgtgct
      841 cggcggtcca ctaccggacg ctcgccggca ccagcgtcaa gcgcgacttc gtcgagttcc
      901 cgagccagct ccacgagcgc tggctcgcga cgcccgaggt gctcggccgg ttcgcgcggc
      961 actaccagac cggcgcgccg atccccgacg agctggtcgc caagatcgag cgcgcgcggc
     1021 acttcaacca ggggttcgcg accgtcgagt acctggcgtc ggcgatctac gacctcgaga
     1081 tccacaccgc gccggccgat ccgcagatcg atccgatcgc gttcgagcgc cgcgtgatgg
     1141 ccgagatcgg ctgcccgccg gagatcgtca tgcgccaccg gccgacccag ttcgcccaca
     1201 tcttcgcgag cgatggctac tccgccggct actacgccta tctgtgggcc gacacgctgg
     1261 tcgccgatgc cgccgaggcg ttcaccgagg ccggtagctt ctacgatccg gcgatcgccc
     1321 ggcggctgtt cgactcggtg atgcgcgtcg gcaactcggt cccgccgcac caggcgttcc
     1381 acgcgttccg cggccgcgac gtcgataccg acgcactgat gcgcgatcgc gggttccccg
     1441 tggtgtgaaa ttcggcgacg atgccgcatg ccgttcggtg gggtgcgcgc tgcgccgctc
     1501 gtcaggctcg ccgccgggcc cgtcgggctc gcgccggcgc tcgcggcgat cctggcctct
     1561 gga
//
LOCUS       DASVOR010002102         2943 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_139201,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002102 DASVOR010000000
VERSION     DASVOR010002102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139201"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2219)
                     /locus_tag="VF516_39005"
     CDS             complement(<1..2219)
                     /locus_tag="VF516_39005"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX8113805.1"
                     /translation="MADTPQLDTVTPASARAGAQLTLAGAGFGDQTPGSAVRFRLPEP
                     GAAAVDGPIVDWADATIHVQVPPLASFGSGGPLELVVHSDAGDSGAVAFLLEEDAPPA
                     ITAVTPARGLEGEPIAITGDRFGRQTADSAVVFQAPGPVDVPAEVVSWIPGSITVRVP
                     SLASLGGAGPRSLAVRTAWGRSASAAFLLGQLPVVASVDPPSPSPGATITVRGSAFGP
                     RPPGRLDLVAVSDIQDPPPNTTAPPILSWTDTEIQARLPELQELRTTGLRDAIVTSEW
                     GASIPDPHSRFLIESRASITCWTRVEPHARTADLQQGLELGLQAQVYDALWLLGRQWQ
                     LLELKGQDAGSPVTVQVNGTCTPLARWQPHGGQPEDVLAGVPLEAVVERERVIPPLGT
                     AGGPFGDLRLAAEAGLQLLRMIDAQLRDPRKSDDYRKRFLRDYPLDVPGDAASLDARS
                     RRFLSVAAQRVPDGSRIYADFQVALGPNPKLPKKPPIDGNDRSGVLAAIGAWYTWCDG
                     LFSESTPGQRAWDPGRMEYSFAAGSGSVVLDASEHDGGHLDWYSFVRRAPGSTLGAPA
                     AGRGPIAFTRSAIPNPVSYPGMPVPRWWELEDRRVDFGSVAAGPSELLKLVLIEFATV
                     YGNDWFTLPLDALPVGSLCEIGAVTVTDAFGVSINLAPFGDGAGTDWRMFELSRDDGA
                     ANAGNALLLLDSLPTTLESGPIEDVVMLRDELANMAWAVEKVIESRTGRPLDRHEDEV
                     "
     gene            complement(2231..>2943)
                     /locus_tag="VF516_39010"
     CDS             complement(2231..>2943)
                     /locus_tag="VF516_39010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113806.1"
                     /translation="AAARIQAVLGGDFPAVPRYTPPPRGDRAQVDVGFATSATLQGGD
                     AFAAALWFQRLTRVRDGARRLGDMLLYADALGGADPLRLAVAQLPAVTGDRWIALPFS
                     GTTPPAGRVSLVAHLPAGALDPARPLVGLLVEQFTEVLPAPAKTTGLAFHYDQPNATP
                     PQAIVLAVPPRPGEDWTLAALETAVQDALDLAKLRMVDLDALQQAGHFVPASYLAFNA
                     KGVTVATDFLTGRGTPLA"
BASE COUNT          425 a         1035 c         1090 g          393 t
ORIGIN      
        1 acctcgtctt cgtggcggtc gagcggacgt ccggtgcggc tctcgatgac cttctccacc
       61 gcccaggcca tgttcgccag ctcgtcgcgg agcatgacca cgtcctcgat cgggccggac
      121 tccagtgtcg tcggcagcga gtcgagcaac agcagggcgt tgcccgcatt ggccgcaccg
      181 tcgtctctgg acagctcgaa catgcgccag tccgtgccag cgccatcgcc gaacggggca
      241 aggttgatcg agacgccaaa cgcgtcggtg acggtcaccg cgccgatctc gcacaggctt
      301 ccgaccggta atgcgtcgag tggcagcgtg aaccagtcat tgccatacac ggtggcgaac
      361 tcgatgagca cgagcttgag cagctcgctc ggcccggcag cgaccgagcc gaagtccacg
      421 cgccggtcct cgagctccca ccagcggggc accggcatac ccggatagct gacggggttc
      481 ggaatggcgc tgcgggtgaa ggcgatcgga ccccgccctg cggcaggcgc acccagcgtc
      541 gagcccggcg cgcgtcgcac gaacgagtac cagtcgaggt ggccaccgtc gtgctcgctc
      601 gcatcgagca ccacgctgcc ggagccggcc gcgaacgagt actccatccg cccggggtcc
      661 caggcccgct gccccggcgt ggactcgctg aacagtccgt cgcaccacgt gtaccaggcg
      721 ccgatcgcgg caaggacgcc ggaccggtca ttgccatcga tcggcggctt cttcggcagc
      781 ttggggttgg ggccgagcgc cacctggaag tcggcataga tgcgcgatcc atccggtacg
      841 cgctgggcag ccacgctcag gaaccggcgg ctgcgcgcgt cgagcgacgc cgcgtcgccg
      901 ggcacatcca ggggatagtc gcgcaggaac cgcttgcggt aatcgtcgct cttccgcgga
      961 tcgcgcagct gcgcgtcgat catgcgcagc agctggagac cggcctcggc ggcgagtcgc
     1021 aggtcaccga acgggccgcc ggccgttccg agcggtggga tcacgcgctc gcgctccacg
     1081 accgcctcca gcggcacgcc ggcaaggacg tcttcgggct ggccaccgtg gggctgccag
     1141 cgcgcgagtg gggtgcacgt gccgttgacc tggacggtca ccggcgagcc cgcgtcctgg
     1201 cccttcagct ccaggagctg ccattgccgc cccagcagcc agagcgcgtc atagacctgc
     1261 gcctgcaagc cgagctcgag cccctgctgg aggtcggccg tgcgcgcgtg cggttccacc
     1321 cgtgtccagc acgtgatcga ggcgcggctc tcgatcagga agcggctgtg cggatcgggg
     1381 atgctcgcgc cccactcgct ggtcacgatg gcgtcgcgga ggccggtcgt acgcagctcc
     1441 tgaagctccg gcagcctggc ctggatctcg gtgtcggtcc acgacaggat cggcggcgcg
     1501 gtcgtgtttg gcggcgggtc ctggatgtcg gacacggcca ccaggtccag gcggcccggc
     1561 ggccgcggtc cgaacgccga gccccgcacc gtgatggtgg cgcccggcga tgggctcggc
     1621 gggtcgaccg atgcgaccac cggcagctgg ccgagcagga acgccgcgga ggcgctgcgt
     1681 ccccaggcgg tgcggaccgc gaggctgcgc gggccggcgc cgccgagcga cgcgagcgaa
     1741 gggacccgga cggtgatcga ccccgggatc cagctcacca cctcggcggg cacgtcgacc
     1801 gggccgggcg cctgaaagac gaccgcggag tcggcggtct gccggccgaa gcgatcgccg
     1861 gtgatcgcga tgggttcgcc ctcgagcccg cgcgccgggg tcaccgcggt gatcgcgggt
     1921 ggcgcatcct cctcgagcaa gaacgcgacg gcgccgctgt cgcccgcgtc gctgtgcacg
     1981 acgagctcca gcggaccgcc cgatccaaac gacgcgagcg gcgggacctg gacgtggatc
     2041 gtggcgtcgg cccagtcgac gatcgggcca tccaccgcgg cggcgccggg ctccggcagg
     2101 cgaaaccgga ccgcggaccc tggggtctga tcgccgaacc cggcgcccgc cagcgtgagc
     2161 tgggcaccgg cgcgagccga tgccggggtg actgtatcga gctgtggcgt gtcggccatg
     2221 tcacatcctc tcaggccagc ggcgtgcctc gccccgtcag gaagtcggtg gccacggtca
     2281 cgcccttcgc gttgaacgcc aggtagctgg ccggcacgaa gtgcccggcc tgttgcagcg
     2341 catccagatc gaccatccgc agcttcgcga gatcgagtgc gtcctgcacg gcggtctcca
     2401 gcgccgccag cgtccagtcc tcgccgggcc gcggcggcac ggccagcacg atggcctgcg
     2461 gcggggtggc attgggctga tcgtagtgga atgccagtcc ggtggtcttc gcgggtgccg
     2521 gcaggacctc ggtgaactgc tcgaccagga ggccgaccag cgggcgagcc gggtccagcg
     2581 ctccggcggg gagatgcgcg accagcgaca cgcgcccggc gggtggtgtg gtgccgctga
     2641 acggcagcgc gatccagcga tcgccggtga ccgccgggag ttgcgcgacc gcgaggcgga
     2701 gcggatcggc gccgccgagc gcatcggcgt agagcagcat gtcgccgagg cgccgcgcgc
     2761 cgtcacgcac ccgggtcagg cgctggaacc acagggcggc ggcgaatgcg tcgcctccct
     2821 gcagggtggc gctggtcgcg aagccgacgt cgacctgcgc gcgatcgcct cgcgggggtg
     2881 gggtgtagcg cggcacggcg gggaagtcgc caccgagcac ggcctggatg cgggctgccg
     2941 cgt
//
LOCUS       DASVOR010002103         3172 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_430773,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002103 DASVOR010000000
VERSION     DASVOR010002103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_430773"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VF516_39015"
     CDS             <1..265
                     /locus_tag="VF516_39015"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8113807.1"
                     /translation="AHAGELTLPRLGLLAAGSIALGAASGVAFTVGLIRVGSARAAVL
                     TFIEPLVAVGVGAAVWGEPLSPLAVVGGTLVLGAGIEVARKAR"
     gene            275..1300
                     /locus_tag="VF516_39020"
     CDS             275..1300
                     /locus_tag="VF516_39020"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8113808.1"
                     /translation="MQPRVTIAMPAYNEEHYIEACIASVQAQDYPRNLIEILIADGRS
                     TDRTREIIAALAAADPRIHLVDNPARLQAAALGLMVKQATGDVVVRMDVHCEYAPDYV
                     RKCVEVLERTGADNVGGSQRAKARTAFQRALCAALASPLGVGGARSRSPDAEGYVDTV
                     FLGAFRRRVFETVGLWDPGAITNEDAELNQRILDSGGQIYLSREIVVHYFPRDSFKTL
                     ARQYYKYGIGRARTLLKLGSFPNLRPALPFLMVAGGTLLLVVPPLWPLAPVAFATYAL
                     ATGAEAVRVGAQLGPAAIPTIWAIFPTLHLSHGVGFAAGLLRYLRKPDWPAEPERIAR
                     RVTLRAV"
     gene            complement(1316..2953)
                     /locus_tag="VF516_39025"
     CDS             complement(1316..2953)
                     /locus_tag="VF516_39025"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8113809.1"
                     /translation="MRPSYAHGTSTVPLLGDTIGETFRRTVDRFPDREALVVRSQGYR
                     ATYRALWDAVDEIARGLLALDIQRGDRVGVWSPNRFEWVVLQYATARIGAILVNINPA
                     YKSSELGYVLNQAGVSVLAMSRGFRHNDYVAMLAEVRAGCTALREVLVFEDDWAKLIA
                     AGKAVSAERLAAREATLAFDDPINIQYTSGTTGFPKGATLSHHNVLNNGYFIAQTLRY
                     TERDRVCVPVPFYHCFGMVIGNLASVTCGACVVVPGEAFDPLLVLETVAAEQCSSLYG
                     VPMMFIAELDHPSFASHDLASLRTGVMAGSSCPIEVMRAVQARMGMREVTICYGMTET
                     SPVSTQSATDDPLDKRVTTVGRVHPHVEIKIIDPETGRIVPRGASGELCTRGYSVMLG
                     YWNDDKATRAAIDAGRWMHTGDLATMDDEGYVNIVGRIKDMIARGGEKVFPREVEEYL
                     HTHPAVAEAQVVGVPSVKYGEEVMAWVRPKTGATLTPEELTAFCTGKIATFKIPRYWK
                     ITDQFPMTVTGKIQKFKMREIAIAELGLGDAAAIKTA"
     gene            complement(2992..>3172)
                     /locus_tag="VF516_39030"
     CDS             complement(2992..>3172)
                     /locus_tag="VF516_39030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113810.1"
                     /translation="IVVERAVGIATADLVRDAARLLGFSRTTERVVERVATGVRLAAQ
                     RELIRIEGGRATLPD"
BASE COUNT          426 a         1166 c         1106 g          474 t
ORIGIN      
        1 cgcgcacgca ggcgagctca ccctgccccg gctcggcctg ctcgcggctg gctcgatcgc
       61 gctcggcgcg gcgtccgggg tcgcgttcac cgtcggcctg atccgggtcg gcagcgcgcg
      121 tgcggccgtc ctcacgttca tcgagccgct ggtcgcggtc ggggtcggcg ccgcggtgtg
      181 gggcgagccg ctgtccccgc tcgccgtggt cggaggcacc ctcgtgctcg gcgccggtat
      241 cgaggtcgcc cgcaaggcgc gctaacataa ggccatgcag ccgcgcgtca cgatcgcgat
      301 gcccgcctac aacgaggagc actacatcga ggcctgcatc gccagcgtgc aggcccagga
      361 ctaccccagg aacctgatcg agatcctgat cgccgacggc cgctcgaccg atcggacccg
      421 cgagatcatc gccgcgctcg ccgccgccga cccgcggatc cacctggtcg acaacccggc
      481 gcggctgcag gccgcggccc tgggcctgat ggtcaagcag gcgaccggcg acgtcgtcgt
      541 ccggatggac gtccactgcg agtacgcgcc cgactacgtg cgcaagtgcg tcgaggtcct
      601 cgagcggacc ggcgccgaca acgtcggcgg ctcgcagcgc gccaaggcca ggaccgcgtt
      661 ccagcgcgcg ctgtgcgccg cgctcgccag cccgctcggc gtcggcggcg ctcgctcccg
      721 ctcgcccgac gccgagggct acgtcgacac cgtgttcctc ggcgcgttcc gccgccgcgt
      781 gttcgagacc gtcggcctgt gggaccccgg cgcgatcacc aacgaggacg ccgagctcaa
      841 ccagcgcatc ctcgacagcg gcggccagat ctacctgtcg cgcgagatcg tcgtgcacta
      901 cttcccgcgc gactcgttca agacgctcgc caggcagtac tacaagtacg gcatcggccg
      961 cgcccgcacc ctgctcaagc tcggctcgtt ccccaacctg cggccggcgc tgccgttcct
     1021 gatggtcgcc ggcggcacgc tgttgctcgt cgtcccgccg ctgtggccgc tcgccccggt
     1081 ggcgttcgcg acctacgcgc tggcgaccgg cgccgaggcc gtccgggtcg gggcccagct
     1141 cggccccgcc gcgatcccga cgatctgggc gatcttcccg acgctgcatc tctcgcacgg
     1201 cgtcggcttc gccgcgggcc tcttgcgcta cctgcgcaag cccgactggc cggccgagcc
     1261 cgagcggatc gcccggcggg tcacgctgcg cgccgtgtag ccggccgcgc cggcgctacg
     1321 cggtcttgat cgcggcggcg tcgcccagcc cgagctcggc gatcgcgatc tcgcgcatct
     1381 tgaacttctg gatcttgccg gtcaccgtca tcgggaactg gtcggtgatc ttccagtagc
     1441 gcgggatctt gaaggtcgcg atcttgccgg tgcagaacgc ggtgagctcc tcgggggtga
     1501 gcgtggcccc ggtcttcggc cggacccagg ccatcacctc ctcgccgtac ttgacgctcg
     1561 gcacaccgac gacctgggcc tcggcgaccg ccgggtgggt gtggaggtac tcctcgacct
     1621 cgcgcgggaa caccttctcg ccgccgcgcg cgatcatgtc cttgatccgg ccgacgatgt
     1681 tgacgtagcc ctcgtcgtcc atcgtcgcga gatcgccggt gtgcatccag cgcccggcgt
     1741 cgatcgccgc gcgcgtcgcc ttgtcgtcgt tccagtagcc gagcatgacg ctgtagccgc
     1801 gggtgcacag ctcgccgctc gcgccgcgcg gcacgatccg gccggtctcg gggtcgatga
     1861 tcttgatctc gacgtggggg tggacgcggc cgaccgtggt gacccgcttg tcgagcggat
     1921 cgtcggtcgc gctctgggtc gacaccggcg aggtctcggt catgccgtag cagatcgtga
     1981 cctcgcgcat gcccatccgg gcctggaccg cgcgcatgac ctcgatcggg cacgacgagc
     2041 ccgccatcac gccggtgcgc aggctcgcca ggtcgtgcga cgcgaacgac ggatgatcga
     2101 gctcggcgat gaacatcatc ggcacgccgt acagcgagct gcactgctcg gcggcgacgg
     2161 tctcgagcac gagcagcgga tcgaacgcct cgccgggcac gacgacgcac gccccgcagg
     2221 tcacgctcgc gaggttgccg atcaccatgc cgaagcagtg gtagaacggc accggcacgc
     2281 acacccgatc gcgctcggtg tagcgcaggg tctgggcgat gaagtagccg ttgttgagca
     2341 cgttgtggtg cgacagcgtc gcgcccttgg ggaacccggt ggtgcccgac gtgtactgga
     2401 tgttgatcgg gtcgtcgaac gcgagcgtcg cctcgcgcgc cgcgagccgc tcggcggaca
     2461 ctgccttgcc ggccgcgatc agcttcgccc agtcgtcctc gaacaccagg acctcgcgca
     2521 gcgcggtgca gccggcgcgg acctcggcga gcatcgcgac gtagtcgttg tggcgaaacc
     2581 cgcgcgacat cgccagcacg ctgaccccgg cctggttgag cacgtagccg agctcgctcg
     2641 acttgtaggc cgggttgatg ttgaccagga tcgcgccgat ccgcgcggtc gcgtactgca
     2701 gcacgaccca ctcgaagcgg ttgggcgacc acacgccgac ccgatcgccg cgctggatgt
     2761 ccagcgcgag caggccgcgc gcgatctcgt cgaccgcgtc ccacagcgcg cggtaggtcg
     2821 cgcggtagcc ctgcgagcga acgaccagcg cctcgcgatc cgggaaccga tcgacggtgc
     2881 ggcggaacgt ctcgccgatg gtgtcgccga ggagggggac cgtgctcgtg ccgtgggcgt
     2941 aggagggccg catggccggc tacgttaccg cgactgggcc cggccgggcg atcagtccgg
     3001 caacgtggcg cggccgccct cgatccggat cagctcgcgc tgggcggcga gccggacgcc
     3061 ggtcgcgacg cgctcgacca cgcgctcggt ggtccgcgag aacccgagca ggcgcgcggc
     3121 gtcgcgcacc aggtcggcgg tcgcgatgcc gaccgcgcgc tcgacgacga tc
//
LOCUS       DASVOR010002104         3770 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_548786,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002104 DASVOR010000000
VERSION     DASVOR010002104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548786"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..2735
                     /locus_tag="VF516_39035"
     CDS             306..2735
                     /locus_tag="VF516_39035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8113811.1"
                     /translation="MVLALGISITAQGAAVVGYVVTRPAPKPTLTVRLGEAASSWDYE
                     LSEHPAPAAAVRVDGPNGFKVTLTTPAGEMVGAMSQSIRLSERTLDARITVIAPDGAQ
                     WSRKIPVKPGQETVVSLDYTPSLGQVLVTRAGDKAPMMRCDQPRCGIELVPGTQVKLT
                     AVLGEGATFGGYRQLPMRTPPALQGILGDPLAACVVRDAVKAASDGSVFECALTVTAD
                     IEVAADFGHQPKEVDVALEPPQLEQLVKPLTPRPPLAPLDAEKLDEPPLQVALKPPPR
                     PDQKPLTVPPPPPPPPPQPEQKKTPPPPPPPNMVMVEVKDDKHVVDKAPDDAKQLSDK
                     NRDVAEETRAKQTNLDKESEGKEVASRESDDHTSQDIGGPDDRIRQLEEAQATTDKHI
                     RETDHSGKDEVAKGAIKGEGGQNGDEGTGENPAVLAMRGIGGRGSIVDNGKDGKKIGK
                     HGTPGINTALTFKDYERLMGKDKVDEERQVAARKMTSKKGRWERKLDAIKSSLENFIP
                     DVRPGNQTALKTRAHPFALYVARMHRRIHELWGFGFLENLDGKGADYPLNDQNLWVNL
                     ELSVNPDGTVHKVTIAKTSGKTEFDVAAVDTAISAGPYEPTPEAIRSVDGRIYLRWGF
                     YRNWRQCGTFNVEPYILTEVPDDGGQGVLDDGAMVKNTAKLPGKRRTDRPAMGTKTVT
                     PDDGLAQQKVSPDSSVTDKQALFAANLWVSAFATGQVEKLVGYSRTPFYAGGKVVAQS
                     TAELKDMYSGLVVESGAMKDWKLLTPAEAGVQDAPEGTFVLQIRTAKEAFSVVLARTP
                     SGEYRATQLGR"
     gene            complement(3023..3235)
                     /locus_tag="VF516_39040"
     CDS             complement(3023..3235)
                     /locus_tag="VF516_39040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX8113812.1"
                     /translation="MALGTVKWFNDAKGYGFISRADDGKDVFVHYSSISGDGFRTLNQ
                     GQTVEYEEQQGPKGLFAAKVLKVMSS"
BASE COUNT          653 a         1315 c         1270 g          532 t
ORIGIN      
        1 gtttcagccg gcgaactcga acatgcagcc gattctcgtt cgcaaggacg acttcaagag
       61 cgttgacatc atcggcgtgg tggttggcgt gtaccgcaag atctgacgat cgcttggctt
      121 ttgtggccgt cgcggccgat gtctcggcgc gtcgatcgcc aggtctaacg acggttcggc
      181 gtccgtcagc tagcaaacgc tagcccggcg atttgtcagc atatttcgcg gcgaccgttt
      241 ggggtttggt atgtcagact caccccgcgg tggtccgctt gcgtcgatat cggagctcag
      301 gcctcttggt cctggcgctg ggcatctcga tcaccgcgca gggcgctgcg gtcgtcgggt
      361 acgtcgtcac gcgcccggca ccgaagccga cgctgacggt ccgtctcgga gaggcggcga
      421 gcagctggga ctacgagctc tccgagcatc cagcacccgc cgctgcggtt cgggtcgacg
      481 ggcccaacgg cttcaaggtc acgctgacga cgccggcggg cgagatggtc ggcgcgatgt
      541 cgcagtcgat ccggctctcc gagcgcacgc tcgatgcgcg gatcacggtg atcgcgcccg
      601 acggcgcgca gtggtcgagg aagatcccgg tcaagcccgg gcaggagacc gtggtctcgc
      661 tcgactacac gccgagcctg gggcaggtgc tggtgacccg cgccggcgac aaggcgccga
      721 tgatgcgctg cgaccagccg cgctgcggga tcgagctcgt gccgggcacg caggtcaagc
      781 tgaccgcggt gctcggcgag ggcgcgacgt tcggcggtta tcgccagctg ccgatgcgca
      841 cgccaccggc gctgcagggc atcctcggcg acccgctcgc cgcgtgcgtc gtccgcgacg
      901 cggtcaaggc ggcgagcgac ggctcggtgt tcgagtgcgc gctcaccgtc accgcggaca
      961 tcgaggtcgc ggccgacttc ggccaccagc ccaaggaggt cgacgtcgcg ctggagcccc
     1021 cgcagctcga gcagctggtc aagccgctga cgccccggcc gccgctggcg ccgctcgacg
     1081 ccgagaagct cgacgagccg ccgctccagg tcgcgctcaa gccgccgccc aggcccgacc
     1141 agaagccgct gacggtccct ccgccgcctc ctcctccgcc gccgcagccc gagcagaaga
     1201 agacgccgcc gccacccccg ccgccgaaca tggtgatggt cgaggtcaag gacgacaagc
     1261 acgtcgtcga caaggcgccc gacgacgcca agcagctgtc ggacaagaac cgcgacgtcg
     1321 ccgaggagac ccgcgccaag cagaccaacc tcgacaagga gtccgagggc aaggaggtcg
     1381 cgtcgcgcga gagcgacgat cacaccagcc aggacatcgg cggacccgac gaccggatcc
     1441 ggcagctcga ggaggctcag gcgaccaccg acaagcacat ccgcgagacc gaccactcgg
     1501 gcaaggacga ggtcgccaag ggcgcgatca agggcgaggg cgggcagaac ggcgacgagg
     1561 gcaccggcga gaaccccgcc gtgctcgcga tgcgcggcat cggcggccgg ggctcgatcg
     1621 tcgacaacgg caaggacggc aagaagatcg gcaagcacgg cacgcccggc atcaacacgg
     1681 cgctgacgtt caaggactac gagcggctga tgggcaagga caaggtcgac gaggagcgcc
     1741 aggtcgccgc gcgcaagatg accagcaaga agggccggtg ggagcgcaag ctcgacgcga
     1801 tcaagagctc gctggagaac ttcatccccg acgtccggcc cggcaaccag accgcgctca
     1861 agacccgcgc gcatccgttc gcgctctacg tcgcgcgcat gcaccgccgg atccacgagc
     1921 tctggggctt cgggttcctc gagaacctcg acggcaaggg cgcggactac ccgctcaacg
     1981 accagaacct gtgggtcaac ctcgagctgt cggtcaaccc cgacggcacg gtccacaagg
     2041 tgacgatcgc caagacctcg ggcaagaccg agttcgacgt cgccgccgtc gacaccgcga
     2101 tctccgccgg cccctacgag ccgacccccg aggcgatccg ctcggtcgac ggccggatct
     2161 acctgcgctg ggggttctat cgcaactggc ggcagtgcgg cacgttcaac gtcgagccct
     2221 acatcctcac cgaggtgccc gacgacggcg gccagggcgt gctcgacgac ggcgcgatgg
     2281 tcaagaacac cgccaagctg cccggcaagc gcaggaccga ccggccggcg atgggcacca
     2341 agacggtcac gcccgacgac ggcctcgccc agcagaaggt ctcgccggac tcgtcggtga
     2401 ccgacaagca ggcgctgttc gccgccaacc tgtgggtctc ggcgttcgcg accgggcagg
     2461 tcgagaagct cgtcgggtac tcgagaacgc cgttctacgc cggcggaaag gtcgtcgcgc
     2521 agagcaccgc cgagctcaag gacatgtact cggggcttgt cgtcgagtcc ggtgcgatga
     2581 aggactggaa actactcacg cctgcggagg caggtgtgca ggacgcaccc gagggcacct
     2641 tcgtgctgca gatccgcacg gcgaaagagg cgttctccgt ggtcctggcc cggacgccgt
     2701 ccggcgagta tcgcgcgacc cagctcggcc gctagccccg taggcgccgt cacgtgcctc
     2761 ggcgccggcc tcgggcacat gcctccggcg cgtgcctccg gcgcgtctgc gcagccctgg
     2821 gacaccctgg gaaccctcgg gcagccggag ggttccctgg gcagcctgca ggacggaccg
     2881 ctgacgctac cctccccgag gtaggtcaga ggtaggccgg aggtacgccg gaagtacgag
     2941 cgatcgagcc cgacgtggga tcgacgtggg atcgacgtgg gatcgacgtg ggatcgacgc
     3001 aggacgagcc ggcgaggccg ccctagctcg acatcacctt gaggaccttc gcggcgaaca
     3061 gccccttggg cccttgctgc tcctcgtact cgacggtctg gccctggttc agggtccgga
     3121 agccatcccc cgaaatcgac gagtaatgga cgaacacgtc cttgccatca tcagcccggc
     3181 tgatgaagcc gtagcccttg gcatcgttga accacttcac ggtaccgagc gccatgtgta
     3241 ctgcctccta cacaacgttg aagtccacct tggactccac gtaatcctgt tcaaacatcc
     3301 cgcgcctcga tcgctcaacc aaattctccg ggaaagcgcc cggactctgc aaactttcat
     3361 cgttacggaa accgtcgaca ccgtcgggtg gcccggctca ctctcgtcgt cgtcgcccca
     3421 agactttttg cagggcatcg cgcgttggct ccacgacgac atgcgctgtt ggctctggct
     3481 ctgcctggtt cttgccgcat gtggggctga ctccgctcct gcgccggtct ccgcaccgcc
     3541 ggccccgcct gcgaagccca ccgcggcgcc gccactgcca ctgagcgcgg cacatggcgc
     3601 ggacatcacg gtgctcggga tcaccgtcga cgggctggcg gctgcgaccg ccgaccggct
     3661 gggcggaatc cggctgtggc ctgtgctcga cggcacgcgt gagccggtgg tgatccaggg
     3721 cacgccgccg cgcgcgatcg cgctgctgcg cgacggcgac ggcgacggct
//
LOCUS       DASVOR010002105         4218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_579084,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002105 DASVOR010000000
VERSION     DASVOR010002105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4218
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_579084"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VF516_39045"
     CDS             complement(<1..255)
                     /locus_tag="VF516_39045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8113813.1"
                     /translation="MTDPEQAAFAAACEAHRRRLLVHCYRMTGSVDEAEDLVQDTLLR
                     AWRARESFEGRARLSTWLHRIATNVCLDALAQRPRRVLPQD"
     gene            513..1130
                     /locus_tag="VF516_39050"
     CDS             513..1130
                     /locus_tag="VF516_39050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113814.1"
                     /translation="MQGFCELPGSGDAGGDDAGVDGPGNASFCLGDAVVMACLAQMPA
                     APLAISSPTMIDTDQPGNCSPDAVGSSASYCIVAGSSITILGGQTLSAHGSRPLVLMS
                     SGAITVIGTIDVAGHLMGVPQATGPGVPGPGSPCASATAATNIAGGSGGSFGSIGGGG
                     GADFNANPGGVPSAAVVPATLRGRRCRAPPSACTSGAPEHCSEEG"
     gene            1367..2680
                     /locus_tag="VF516_39055"
     CDS             1367..2680
                     /locus_tag="VF516_39055"
                     /inference="COORDINATES: protein motif:HMM:NF024700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8113815.1"
                     /translation="MSALLGIRIQNFKSLADVQLGQVAYGQGQPLPRFACFIGPNGSG
                     KSSLLDAFGFIADCLLEGVEAACDKPHRGGFERLRTQGSTGPMSFELFFVDDDPARPI
                     VYKFLVDVGAEAVPTVIEESLRQRRKGQTKGKPYYFLKLENGAGKVWAGDYVGDDTDD
                     PHAAPAQLADANRLGLTTLGNLAEHPRIVRLRTYIEQWYLSYFIPNAARKQPPAGAQR
                     WLDPDGANIANVLQYYQRTHPDRFEGIVARMAKGIPGIRSIVPGISEDKRLLLKFNEE
                     GYRDPFYQQSMSDGTLKMLAYGVLLEDPEPRPLIGIEEPENGLYIELIERLAVSFVEH
                     TARPRAPTQIFVTTHSPYFVDALQPEQVWLMGKRGGRSAAVRVADLEKVRALSRQGIP
                     LGAQWYSHHFDESPAPTRKIATSTAARQHTAETSVRKRRSKRGRR"
     gene            2692..3363
                     /locus_tag="VF516_39060"
     CDS             2692..3363
                     /locus_tag="VF516_39060"
                     /inference="COORDINATES: protein motif:HMM:NF025468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4276 family protein"
                     /protein_id="HEX8113816.1"
                     /translation="MVEGPSEKVLLDLWAPRAFPGHQFLVKPHQGKGTLPNARARPDP
                     RKRGLLDLLPATLRAYSESCSDDEAVLVLVDADDDDCEHLLRRMRALAAEVCPKLRVV
                     FRIAIEETEAFYLGDLRAIKAAFPEANVERARELIPDSRPPKGTAEAFAEIVGDDTLR
                     KVEWAERIGPKLTIHPEQSRSLSFQKLHAGIHRLVSQRPAPKRRRAKHWKARYSSQRK
                     QSVRR"
     gene            complement(3420..>4218)
                     /locus_tag="VF516_39065"
     CDS             complement(3420..>4218)
                     /locus_tag="VF516_39065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113817.1"
                     /translation="VPVLAHTFSHLPMFDEAVDGDLVALIYSVRHGKDSMVAIAGIVF
                     GAVVLWQLAAWFLVGGAYSVLAQRPEGRGDTARCFGAGGASTYLAYARLALCALPGWL
                     AVLFVLGFGLGLVKGRIDHALTLGELFGALTLAVLPAALLLHLLWTVEDYARVELTLR
                     HDTHDPGVVATYVRTLSYVFRRPVTLVHGALGWLVFGLVSTAYFYLSLGHPMYGAEGA
                     ITLFIARQGVSLLRMAIRFGVLAGQVELGRTRALPPRRIEVKVDPKP"
BASE COUNT          785 a         1306 c         1419 g          708 t
ORIGIN      
        1 gtcctgcggc agcacccggc gcggccgctg cgcgagcgca tcgaggcaca cgttggtcgc
       61 gatccggtgc agccaggtcg acagccgggc gcggccctcg aagctctcgc gcgcccgcca
      121 cgcgcgcagc aaggtgtcct gcaccaggtc ctcggcctcg tccaccgagc cggtcatccg
      181 gtagcagtgc accagcagcc ggcgccggtg cgcctcacat gcagcggcga acgcggcttg
      241 ctcgggatcg gtcacggctc gcgaatacca cgccgcgcga ggcgcgtcag ggagctcacg
      301 tcgccggggc ggaacacggt ctgcacggct tctcggtcgt ggcgtcgatc accacagcgc
      361 cacgccacgt caccgtgcgg ccagcgtagt atcggcacgt gcctcgctgg gctcgtcatg
      421 cggtggtcct gctgcttccc gcctgctttc atcccagcta cgaccatacg gcgtgtagcc
      481 tcgacggcgc ctgcccgagc ggttggcatt gcgtgcaggg cttctgtgag ctgccgggca
      541 gtggcgacgc gggcggtgac gatgcgggcg tcgacggccc gggcaacgcg agcttctgcc
      601 tcggcgacgc cgtcgtcatg gcctgccttg cgcagatgcc cgcagcgccg ctcgcgatca
      661 gcagcccgac gatgatcgat accgaccagc ctgggaactg ctcgccggac gcggtcggtt
      721 cctcggcgtc gtactgcatc gtcgccggga gctcgatcac gatcctggga gggcagaccc
      781 tgagcgcgca cggcagccgg ccgctcgtgc tgatgtcgag cggcgcgatc acggtgatag
      841 ggacgatcga cgtggccggt catctgatgg gggtgccgca ggcgacgggg cccggggtac
      901 cgggaccggg cagcccgtgc gcgtccgcga cggccgccac caacatcgca ggtggcagcg
      961 gtggcagctt cggctcgatc gggggtggtg gcggtgccga cttcaacgcc aatcccggcg
     1021 gcgtgccgtc ggcagccgtc gtaccggcca cgctgcgcgg tcgccggtgt agagcaccgc
     1081 cgtcggcgtg cacgagcggt gcccccgagc actgcagcga agaaggatag cctctgctgg
     1141 ctccccctcc ccaagaaacg gtgccgagga cacgcagttc agcctatgtg cgacgcgttg
     1201 tagcgcgacg ctgactcgcg gtgccgttgc cgtcaccagc gggcggtcgc gggccccggc
     1261 tgctcgaccg gactcttgtc cgggaagggc catcggacgg catgagatcg acgttgcggc
     1321 tcccacacga cgctcctcat gtcgtgtatc ctgctcaggg cgcttcatgt cagccttact
     1381 aggcatccgc atccagaact tcaagtcatt ggccgatgtc cagctcgggc aggtcgcgta
     1441 tgggcagggg caaccactgc cgcgattcgc gtgcttcatt ggtcctaacg gctccggcaa
     1501 gtcttcgttg ctggatgcgt tcgggtttat cgcggactgc cttctcgagg gtgttgaggc
     1561 cgcatgcgac aagccacatc ggggaggctt cgagcgtttg cgcacacaag gttcgacggg
     1621 accgatgtcg ttcgagttgt tctttgtgga tgatgatccc gctcgcccaa tcgtctataa
     1681 gttcttggtg gatgttggtg ccgaagcagt gcccacggtg atcgaggaat cgttgcgcca
     1741 gcgccgaaaa gggcagacca aagggaaacc gtactacttt ctcaagttgg agaacggtgc
     1801 tggcaaggtt tgggcaggtg attacgtagg agacgatacc gatgatcctc acgcggcgcc
     1861 agcgcaactg gcagacgcca atcgtcttgg gttgacgact ctaggtaacc tcgcagagca
     1921 tcccaggatc gtcaggttgc gcacgtatat cgaacaatgg tacctgtcat acttcatccc
     1981 gaacgccgcg aggaaacaac ctcctgcggg tgcgcagcgc tggttggatc cggatggtgc
     2041 caatatcgcc aacgtacttc aatactacca gcgcacgcat ccagatagat tcgaaggaat
     2101 cgtggcccga atggccaagg gaattccggg tattcgttcg atcgtgcctg gcattagtga
     2161 agacaaacgt ctgcttctca agtttaatga ggaaggttat cgcgacccat tctatcagca
     2221 gagcatgtcg gacggaacgt tgaagatgct cgcatatgga gtcctgctcg aggatcccga
     2281 gccacgacca ctcattggta tcgaggaacc agagaatggc ctgtatattg agctcatcga
     2341 gcggctcgct gtgagctttg tggagcatac cgcgagaccg agagccccta cccaaatctt
     2401 cgtgacaacg cactcaccgt acttcgttga tgcgctgcaa cctgaacagg tctggctgat
     2461 gggaaagcga ggcggacgct cagcggccgt gcgggttgcg gatctggaaa aggttagggc
     2521 actctcccgt caagggattc cgctgggcgc gcaatggtac agtcatcact ttgacgaatc
     2581 accggcaccc acacgcaaga tcgcaacgag tacagctgcc cggcagcata cggcggaaac
     2641 atcggtccgg aagagaagga gcaagcgagg gcgccggtga aagttttcat cttggtcgag
     2701 ggcccgtcgg agaaggtatt gctggacctg tgggctcccc gagcgtttcc ggggcatcag
     2761 ttcctggtca agccgcatca aggcaagggt acgcttccca atgcgagagc acgacctgac
     2821 ccaagaaagc gcggtctcct cgacctgttg ccggcgacat tgcgtgcata ttcagagagc
     2881 tgcagtgatg acgaggctgt gcttgtgctg gtcgatgccg acgatgacga ttgcgagcat
     2941 ctcctgcgta ggatgcgcgc tctggcagca gaggtgtgtc cgaagcttcg tgttgtattc
     3001 cgcattgcga tcgaagagac cgaggcattc tatctcggtg atctgagagc catcaaggca
     3061 gcattccctg aggcaaatgt cgagcgcgcg cgtgaactga ttcccgactc acgaccaccc
     3121 aagggtacgg cagaggcctt cgcagagatt gttggggatg atacgttgcg aaaggtagag
     3181 tgggccgagc gcatcggtcc aaagctcact atccaccccg agcaaagcag gtcgctttcg
     3241 tttcagaaac tgcacgcggg aatccatcgg ctggtgagcc aacgtcctgc accaaaacga
     3301 cgacgtgcga agcactggaa ggcgcggtac tcgagccagc gcaagcagtc ggttcgacgg
     3361 tgagtgacct ggggcgaccg aagcagcgac ccttaagttg cactgacaag ttcacccggt
     3421 cacggcttcg gatcgacctt gacctcgatg cggcggggcg gcagcgcccg ggtccggccg
     3481 agctcgacct ggccggcgag cacgccgaac cggatcgcca tgcggagcag cgacacgccc
     3541 tggcgggcga tgaacagcgt gatcgcgccc tcggcgccgt acatcgggtg gccgagcgag
     3601 aggtagaagt acgcggtgct gaccaggccg aacaccagcc agccgagcgc gccgtggacc
     3661 agcgtgaccg ggcggcggaa cacgtagctg agcgtgcgca cgtaggtcgc gaccacgccc
     3721 ggatcgtggg tgtcgtggcg cagggtcagc tcgacgcgcg cgtagtcctc gacggtccac
     3781 aggaggtgga gcagcagcgc ggcgggcagg accgcgagcg tgagcgcgcc gaacagctcg
     3841 cccagggtca gggcgtgatc gatccggccc ttgaccaggc cgaggccgaa gccgagcacg
     3901 aacagcacgg ccagccagcc gggcagcgcg cacagcgcga gccgcgcgta ggcgaggtag
     3961 gtcgacgcgc cgccggcgcc gaagcagcgc gcggtgtcgc cgcggccctc ggggcgctgg
     4021 gcgagcacgc tgtaggcgcc gccgaccagg aaccacgctg cgagctgcca cagcacgacc
     4081 gcgccgaaca cgatgccggc gatcgcgacc atgctgtcct tgccgtggcg cacggagtag
     4141 atcagcgcga ccagatcgcc gtcgaccgcc tcgtcgaaca tcggcaggtg cgagaacgtg
     4201 tgggcgagga ccgggacg
//
LOCUS       DASVOR010002106         2223 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_314841,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002106 DASVOR010000000
VERSION     DASVOR010002106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_314841"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VF516_39070"
     CDS             complement(<1..624)
                     /locus_tag="VF516_39070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113818.1"
                     /translation="MTTFVTPITHGTSLTRVVPSLAVPALRFADDPVMWIRMPASTSV
                     AAFATGAVARHDDTTNPFPALTGSAAGPWTLFQLSPLPQMNPAAFKAIAGGLPVQLVV
                     VVGTVPASPASNDCLVAGQTLAPAPGGTGAVFLAFAFQDRLCRDPLSWAEAIAASGAC
                     DGNWAQFVTDLAGLPAARNLRILDGRGFPRTTGTVSVSIDGGPVTSHP"
     gene            complement(621..>2223)
                     /locus_tag="VF516_39075"
     CDS             complement(621..>2223)
                     /locus_tag="VF516_39075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113819.1"
                     /translation="IVRDAPGLTAADRALLGAHQNAIVLEWGWRAQERELDPVATEFR
                     VYRTVPRDAVTATITAVSSLGTGWRIAMTTNVALIANELVGQWLTSNDQPFLITQNDA
                     GTAPNVTVELSKLRPAVQPVPGAIVFGRPLRPEHQRPAGWDERVAIVPITADTSYRHV
                     FYDLLTLSPAHPRDAVWVGVSAADAQSYIADERTAGANAPRAGNESAIVTCAVVARYR
                     GQPVFSVPPPLGDVPELVTDEPAGRSILVALDLGALLGGALPAGAPVALARCSSDDLL
                     SRVALSGSDVVLTHPDGSHETIAFPNPGDHAAVVATLGSPDPQRLANRYLLHLVAGSS
                     HPVGLFERVSADIVHVGPVTDRLAPKPGRFLYLVRAADALGNVSDGGAVLPVVVRVPS
                     TANAVRPLRRGLAATATTATLTVAVPADPDTTTVLLFAAVAPPNTNPPVQGEAELLRI
                     PNRRDLYPHDGIRLRLSSGTLLPPAVVKSLADGDVAVETDGTRVVALTTTAATGAWLT
                     LWCFALTRDGLPSPVCGPFGTGVRS"
BASE COUNT          304 a          805 c          855 g          259 t
ORIGIN      
        1 cggatgactg gtcaccgggc cgccgtcgat cgagaccgac acggtgccgg tggtgcgcgg
       61 gaagccccgc ccgtcgagga tccgcaggtt gcgcgccgcg ggcagccccg cgaggtcggt
      121 cacgaactgc gcccagttgc cgtcgcacgc gccgctcgcc gcgatcgcct cggcccagct
      181 cagcgggtcg cggcacagcc ggtcctggaa cgcgaacgcg aggaacacgg cgccggtacc
      241 gccgggggcc ggcgcgagcg tctggccggc gaccaggcag tcgttgctcg ccgggctggc
      301 cggcacggtc ccgacgacga cgaccagctg caccggcagc ccgccggcga tcgccttgaa
      361 cgccgccggg ttcatctgtg gcagcggcga cagctggaac aaggtccacg gcccggcggc
      421 gctgccggtc agcgcgggga acgggttggt ggtgtcgtcg tggcgggcga ccgcgccggt
      481 ggcgaacgca gcgaccgacg tcgacgccgg catccggatc cacatcaccg gatcgtcggc
      541 gaaccgcagc gcggggaccg cgagcgacgg gacgacgcgc gtgagcgacg tgccgtgcgt
      601 gatcggcgtg acgaacgtgg tcatgaccgc acccccgtgc cgaacgggcc acagaccggc
      661 gacggcaggc catcgcgcgt gagcgcgaag caccacagcg tcagccacgc gccggtcgcc
      721 gcggtcgtgg tcagcgcgac cacgcgcgtg ccgtcggtct cgaccgcgac atcgccgtcg
      781 gcgagcgact tcacgaccgc cggtggcagc agcgtgccgc tcgacagccg cagccggatg
      841 ccgtcatggg gatagaggtc gcggcggttg ggaatccgca gcagctcggc ctcgccctgc
      901 accggcgggt tggtgttggg gggcgccacc gcggcgaaca gcagcaccgt cgtcgtgtcg
      961 gggtcggcgg ggaccgcgac ggtcagcgtg gcggtcgtgg cggtggcggc gagcccgcgc
     1021 cgcagcgggc gcaccgcgtt cgccgtcgac ggcacgcgca cgaccaccgg cagcaccgcg
     1081 ccgccgtcgg acacgttgcc gagcgcgtcg gcggcgcgca ccaggtaaag gaaccggccc
     1141 ggcttggggg cgagccggtc ggtcaccggg ccgacgtgga cgatgtcggc gctgacccgc
     1201 tcgaacaggc cgaccgggtg gctcgagccg gcgaccaggt gcagcaggta gcggttcgcg
     1261 agccgctgcg ggtccggcga gccgagcgtc gcgacgaccg ccgcgtggtc gcccgggttg
     1321 gggaacgcga tggtctcgtg gctgccgtca ggatgggtca gcacgacgtc gctgcccgac
     1381 agcgcgaccc gcgacagcag atcgtccgac gagcagcgcg ccagcgcgac cggcgcaccg
     1441 gccggcagcg cgccgccgag cagcgcgccg agatcgagcg cgacgaggat cgaccgcccg
     1501 gcgggctcgt cggtgaccag ctcggggacg tcgccgagcg gcggcggcac gctgaacacc
     1561 ggctggccgc ggtagcgcgc gaccaccgca cacgtcacga tcgcgctctc gttgccggcg
     1621 cgcggcgcgt tggcgcccgc ggtgcgctcg tcggcaatgt acgactgcgc gtcggccgcc
     1681 gacacgccga cccacaccgc gtcgcgcggg tgcgccgggc tgagcgtcag cagatcgtag
     1741 aacacgtggc gatacgacgt gtcggccgtg atcggaacga tcgcgacccg ctcgtcccag
     1801 cccgcggggc gctggtgctc ggggcggagc ggtcgcccga acacgatggc gccggggacc
     1861 ggctggaccg ccggccgcag cttcgacagc tcgaccgtca cgttcggcgc ggtgcccgcg
     1921 tcgttctggg tgatcaggaa tggctggtcg ttgctggtca gccactgccc gacgagctcg
     1981 ttggcgatca gcgcgacgtt ggtcgtcatc gcgatccgcc agccggtgcc cagcgagctg
     2041 accgcggtga tcgtcgcggt caccgcgtcc cgcggcaccg tccggtacac ccggaactcg
     2101 gtcgccaccg gatcgagctc gcgctcctgc gcgcgccagc cccactcgag cacgatcgcg
     2161 ttctggtgcg cgccgagcag cgcgcgatcg gccgcggtga gccccggcgc gtcgcgcacg
     2221 atc
//
LOCUS       DASVOR010002107         1546 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401517,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002107 DASVOR010000000
VERSION     DASVOR010002107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401517"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VF516_39080"
     CDS             complement(<1..1148)
                     /locus_tag="VF516_39080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113820.1"
                     /translation="MTVRSRTWWPASLIGLVVAAILLISLQQGGVDWMVRAVDAMVGE
                     HRAARDRYNEGVAALARGDHDAAEKALLDARSNAGVDPELRFRAAYDLGFAFASHADQ
                     KRIGKDADLAVALELAQQAASWLGDASRLRPDDADTRANLAIARAKVQAISDELRKGE
                     SKLEARLDRLIGEQRGVLDEVRGAWLAIKEAGGSDPLAQQGTLTHLADRERGIVAEAG
                     VVGDLAADEIDAIGKKPEDKRSEQEKIRVIQLKNLDLYLMEGRTRITEARRKLQELAA
                     EDGVARSEAALVALKRAREQLLDPITVMKQVAQDEVAVLQDTARSDTRALELAPGAGA
                     GKDGKDAKAPAVLPGWLEPPVIGEHQGGIRDRLEEVRARLQAAADAPPP"
     gene            complement(1145..>1546)
                     /locus_tag="VF516_39085"
     CDS             complement(1145..>1546)
                     /locus_tag="VF516_39085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113821.1"
                     /translation="DDQDPYSEDAAKLARDAGIKIVAIGLGSETGSEITLTDPQTGAK
                     TKLMYDGKPVISKLDGDRLRKVALVTEGAYIPAGTSAVDLESIMKTHVAPMVSAAVAS
                     AQRVIPAERYTWLVLASLGFLLAALWVGRTP"
BASE COUNT          168 a          576 c          556 g          246 t
ORIGIN      
        1 ggcggtggcg cgtcggccgc cgcctgcagc cgggcgcgga cctcctcgag gcggtcgcgg
       61 atgccgccct ggtgctcgcc gatcaccggc ggctccagcc agccgggcag caccgcgggc
      121 gccttggcgt ccttgccgtc ctttccagct ccggcgcccg gcgcgagctc cagcgcgcgc
      181 gtgtcggagc gcgcggtgtc ctgcagcacg gcgacctcgt cctgcgcgac ctgcttcatc
      241 accgtgatcg gatcgagcag ctgctcgcgc gcccgcttga gcgcgaccag cgcggcctcg
      301 ctgcgcgcga cgccgtcctc ggcggcgagc tcctggagct tgcgccgggc ctcggtgatc
      361 cgggtccggc cctccatcag gtagaggtcg aggttcttga gctggatcac ccggatcttc
      421 tcctgctcgc tgcgcttgtc ctcgggcttc ttgccgatcg cgtcgatctc gtcggccgcg
      481 aggtcgccga ccacgccggc ctcggcgacg atgccgcgct cgcggtcggc gaggtgggtc
      541 agcgtgccct gctgggccag cgggtcgctg ccgccggctt ccttgatcgc cagccacgcg
      601 ccgcgcacct cgtcgagcac gccgcgctgc tcgccgatca gccggtcgag ccgggcctcg
      661 agcttgctct cgcccttgcg cagctcgtcg ctgatcgcct ggaccttggc ccgcgcgatc
      721 gcgaggttgg ccctggtgtc ggcgtcgtcg gggcgcagcc ggctggcgtc gcccagccac
      781 gacgccgcct gctgcgcgag ctcgagcgcg accgcgaggt cggcgtcctt gccgatccgc
      841 ttctggtcgg cgtgcgaggc gaacgcgaac ccgaggtcgt aggccgcgcg gaaccggagc
      901 tcgggatcga cgccggcgtt gctgcgcgcg tcgagcagcg ccttctccgc ggcgtcgtgg
      961 tcgcccctgg cgagcgcggc gaccccctcg ttgtagcggt cgcgggcggc gcggtgctcg
     1021 ccgaccatcg cgtcgacggc gcgcaccatc cagtcgacgc ccccctgctg gagcgagatc
     1081 aggaggatgg ccgcgacgac gagcccgatc aacgatgcgg gccaccaggt gcgcgagcga
     1141 accgtcatgg cgtcctcccg acccacagcg ccgcgagcag gaacccgagc gacgcgagca
     1201 ccagccaggt gtagcgctcg gccgggatca cgcgctgggc ggacgcgacc gcggcgctca
     1261 ccatcggggc gacgtgggtc ttcatgatcg actcgagatc gaccgccgag gtcccggcgg
     1321 ggatgtaggc gccctcggtg accagcgcga ccttgcgcag gcggtcgccg tcgagcttgc
     1381 tgatcaccgg cttgccgtcg tacatcagct tggtcttggc gccggtctgc ggatcggtca
     1441 gcgtgatctc gctgccggtc tcgctgccca ggccgatcgc gacgatcttg atcccggcgt
     1501 cgcgcgccag cttggccgcg tcctcgctgt agggatcctg gtcgtc
//
LOCUS       DASVOR010002108         1832 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_168209,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002108 DASVOR010000000
VERSION     DASVOR010002108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168209"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            180..>1832
                     /locus_tag="VF516_39090"
     CDS             180..>1832
                     /locus_tag="VF516_39090"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113822.1"
                     /translation="MSLAHASRAATLEEILAKNLAARGGEAKLREVKTLRLTGRVMFG
                     GRGRTIEAPWGQVLKRPGMVRSETTLQGLTQISAYDGHDGWTITPFQGRLDAEKASQD
                     EAHALAQQAELDGPLFGWRDKGHRIEYLGTEDTDGTPAIKLRVTRKDGDLQYVFLDPD
                     SYLEIRITTVRKIRGAEHITETDLGGYQQVSGVWIPFAIETGNPGGPRTNRILVEHAE
                     VNVAAEDAWFKLPPPKTRALAVIFPGTTEPNAMVAAAPPPPPSSEKVAFDSGTISGLG
                     ARNIGSATMSGRIAAVAGRVVGGKSLLYVGAASGGVWRSQDGGTTFKPVFDKQPVQSI
                     GAIAIDPSNPETVWVGTGESWMRNSVSVGNGIYKSTDGGETWTHMGLPESEHIVKILV
                     HPTASQVVYACVPGKLWSDSNDRGLYKTTDGGKTWTQVLKGANLSTGCSSVTMDPKNP
                     EVLITGMWDFRRKGWTFRSGGDGPNAPSGSGMFRSTDGGKTWASLATARGLPALPWGR
                     VETVFAPSDPKIVYALIESKASALFRSADGGATWEQRDTSQNM"
BASE COUNT          305 a          640 c          652 g          235 t
ORIGIN      
        1 ccggcggtcc gccgcggcgc ggtgcgcctg cgggcacacc ggccgtgtcc cacccgcacg
       61 gccgcggcac cgctgcgaga ttttcaccag ccggtggcga cgagctattt cctgggctgt
      121 cacgcaggcg cactatcccg cccatgcgaa acctgatcgc gatcgcgata gtgatgctga
      181 tgtcgctagc gcacgccagc cgtgcggcga cgctcgagga gatcctggcg aagaacctgg
      241 ccgcacgcgg cggtgaggcc aagctccgcg aggtcaagac gctgcggctc accggccgcg
      301 tcatgttcgg cggccgcggc cgcacgatcg aggcgccgtg gggccaggtc ctgaagcgcc
      361 cgggcatggt gcgcagcgag accacgctgc agggcctgac ccagatcagc gcgtacgacg
      421 gccacgacgg ctggacgatc acaccgttcc agggccggct cgacgcggag aaggcgtcgc
      481 aggacgaggc ccacgcgctc gcccagcagg ccgagctcga cggtccgctg ttcggatggc
      541 gcgacaaggg ccaccgcatc gagtacctcg gcaccgagga caccgacggc acgccggcga
      601 tcaagctgcg ggtgacccgc aaggacggcg acctccagta cgtgttcctc gatccggact
      661 cgtacctcga gatccggatc accacggtgc gcaagatccg cggcgccgag cacatcaccg
      721 agaccgacct cggcggctac cagcaggtca gcggcgtgtg gattccgttc gcgatcgaga
      781 ccggcaaccc gggcggcccg cgcaccaacc ggatcctcgt cgagcacgcc gaggtcaacg
      841 tcgccgccga ggacgcgtgg ttcaagctgc cgccgcccaa gacccgggcc ctagcggtga
      901 tcttccccgg cacaaccgag ccgaacgcga tggtggccgc cgcgccgcca ccgcctccgt
      961 cgagcgagaa ggtcgcgttc gacagcggca cgatctcggg gctcggcgcg cgcaacatcg
     1021 gctcggcgac gatgagcggc cggatcgccg cggtcgccgg ccgggtcgtc ggcggcaaga
     1081 gcctgctcta cgtcggcgcc gcgtcgggtg gcgtgtggcg gtcgcaggac ggcggcacga
     1141 cgttcaagcc ggtgttcgac aagcagccgg tgcagtcgat cggcgcgatc gcgatcgatc
     1201 cgagcaaccc cgagaccgtg tgggtcggca ccggcgagtc gtggatgcgc aactcggtgt
     1261 cggtcggcaa cgggatctac aagtcgaccg acggcggcga gacctggacc cacatgggcc
     1321 tgcccgagtc cgagcacatc gtgaagatcc tcgtccaccc gacggcgagc caggtggtct
     1381 acgcctgcgt gccgggcaag ctgtggagcg actcgaacga ccgcggcctg tacaagacca
     1441 ccgacggcgg caagacctgg acgcaggtgc tcaagggggc gaacctgtcg accggctgct
     1501 ccagcgtgac gatggatccg aaaaatcctg aggtgctgat caccgggatg tgggacttcc
     1561 gccgcaaggg ctggacgttc cgctcgggcg gcgacgggcc caacgcgccg agcggcagcg
     1621 ggatgttccg ctcgaccgat ggcggcaaga cctgggcgtc gctcgcgacc gcccggggcc
     1681 tgccggccct gccgtggggc cgggtcgaga ccgtgttcgc gccgtcggat cccaagatcg
     1741 tctacgcgct gatcgagtcc aaggcgtcgg cgctgttccg ctcggccgac ggcggcgcga
     1801 cctgggagca gcgcgacacc agccagaaca tg
//
LOCUS       DASVOR010002109         6836 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_284421,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002109 DASVOR010000000
VERSION     DASVOR010002109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6836
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284421"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VF516_39095"
     CDS             complement(<1..377)
                     /locus_tag="VF516_39095"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEX8113823.1"
                     /translation="MQESSSSESSTVRTAIDASSRDLRRVDIHPDHWYPLALSHEVKR
                     GKAHGVRFAGEPIVLVRTASSTVFALEDRCAHRQIPLHDGVVDGETIRCCYHGRTYDC
                     TGRCVHIPYIGRERLPNGVRAYP"
     gene            701..1228
                     /locus_tag="VF516_39100"
     CDS             701..1228
                     /locus_tag="VF516_39100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113824.1"
                     /translation="MDFFALDNTRATLTLVPASNVTMQGFHARFKLRLGDGREELRIV
                     VGNQDARMGEHATAQCTARVARRFPLITVIFQSGRGPARRIACRSCAGGQSRHNRKRG
                     LLRCRFPVSGGSPRGEECLERAIRAALAPHAARIAHLRISRLLVRFATHRIHRRSGNT
                     DESVPAPAWRLLCTI"
     gene            1254..2606
                     /locus_tag="VF516_39105"
     CDS             1254..2606
                     /locus_tag="VF516_39105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113825.1"
                     /translation="MSLLRRPIEHELHQHHFPNRGAPPSPGLVKTPSTDTMWFENAHH
                     DHRHEHPGPFPYGLVQKPSPETVTHTFRHLVRLDGKNNTLPAPAEVIVRTRVERTAGE
                     YVLGDLVYTLSLLPGEEVQVYTSDRRTSFKLDTQTNVAWQASASFEDQMYLSAMDRYF
                     AQLSANEWAQATSSTSGSFNASAETSSALESFFGGGDADMEGSFNVNSVATFLGGMAA
                     QASAAHESSATATNTASALTVGAVGTRTGVQGKIDTHVEMNYRSFRNPSRTRAVTFYF
                     FQIRRRHKVRVTVDKLEVAAPERTLWRMALADAKQTVKAAPLMMPVLGTKHIANIADY
                     QAARETIVNEMVAKKLLEQKDGQLVVPEALRTQTTFEREFTLCTPGIYVMGDRDRTTI
                     ADPSLLRDAWTVGAAIADEVEALAISAVERAGNTIVPAVAGVVRGAQAMLAGASSLLP
                     "
     gene            complement(2704..3432)
                     /locus_tag="VF516_39110"
     CDS             complement(2704..3432)
                     /locus_tag="VF516_39110"
                     /inference="COORDINATES: protein motif:HMM:NF004779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HEX8113826.1"
                     /translation="MDLGLKGKSVLITGGSKGIGLACAGSFAAEGARLHLAARDGDRL
                     NRAKSDLGGDVILHPCDLRDPGALRKLAAECADVDILVNNAGDIPGGTIEMIDDAKWR
                     HAWELKVFGFINLTRELYPRMKARRSGVIVNVIGMAAEQPSFEYVCGAAANAGLANFT
                     RALGKGSREHGVRVVAVHPPATRTDRIITLMKAQAKARFGDESRHQELMGHVIEPEQV
                     ADMVCFLASARASQLSGVVYNLGA"
     gene            3858..4802
                     /locus_tag="VF516_39115"
     CDS             3858..4802
                     /locus_tag="VF516_39115"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8113827.1"
                     /translation="MIENLRAGACVATLGFLLFTACSSTAPAKGKAWESMDRSTLDRA
                     YNNSAAVPEGAQMFKEWQARSAQVREQHPEHLDLPYGPRERNRIDYFSAGPNTPVLIF
                     FHGGYWQMRSKADFAFLAESFLGQGISVAMVGYPLGPEATMDEIVADAHAAVRYLAAQ
                     LPSLGGDPARVVVSGWSSGGHLATSVLDEPSLRGGVAISGLYDLAPLVKSYVNDKLHM
                     DEAAARRNSPMLNLPPSSKPLELFAGSAELPEMRRQTADYASARRMAGLPVHYEEISG
                     ANHYTILNDMLRPEGRIHQALVVMVGITPSVSPPPETR"
     gene            complement(4976..5497)
                     /locus_tag="VF516_39120"
     CDS             complement(4976..5497)
                     /locus_tag="VF516_39120"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8113828.1"
                     /translation="MSSFGRKHGQEGSAGEGAAAGPAPAVGKHALVGATAGNTGAESS
                     PGKHTLVETAMAKMGSGTPVPADVRAGVEAGTGGDLSSATVHAGPAASQIARDVDARV
                     FTFGSQIVLGDSASMSDAQLLAHEATHVVQQAGRSPQRHARYRRPEADQRGDQGVIPS
                     GTCRFSKASRSLE"
     gene            complement(6429..>6836)
                     /locus_tag="VF516_39125"
     CDS             complement(6429..>6836)
                     /locus_tag="VF516_39125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113829.1"
                     /translation="GNRTTTVSLDGARAETIDINPHDGRAFDATVSRDPTTGKETITV
                     TPKIDLELSVDHTVLGDTPPVYDVTQVLLDGSVRTSDASNQIEVATGSFGIATSPAGH
                     GFTASAGQCVTSSSAIDPATSRSYTQYTVAACH"
BASE COUNT         1080 a         2387 c         2299 g         1070 t
ORIGIN      
        1 catggatacg cgcgcacgcc attgggtaag cgctcgcggc cgatatatgg aatgtgcacg
       61 caacggccgg tgcagtcgta ggtccggccg tggtaacagc agcggatcgt ttcgccgtcc
      121 accacgccgt cgtgcagcgg aatctgccgg tgcgcgcagc gatcctcgag tgcgaacacc
      181 gtgctggatg cggtgcgcac gagcacgatc ggctcgcccg cgaaacgcac gccgtgcgcc
      241 ttgccgcgct tcacctcgtg cgacaacgca agcgggtacc aatggtccgg atggatgtcc
      301 acgcgacgca gatcgcgcga cgatgcgtcg atggcagtgc ggacggtgct ggattcggat
      361 gaactggact cttgcatggc gatatccccg acgtgcgacc tctacgaact atgtccgcat
      421 gtcggagagt cgtcgacatg aacgggtgca tcgaccggcc gcggcggcca gcactcagcg
      481 aggaagttca agcgaccgca gatcgatgcg cggcgctcga gcaattcttg ctgcgcgccg
      541 gtgacgcgtt cgctgggcgc ccttgcacgt ggcgcacgct cgaagtccgg agcgctcggc
      601 acgagccggc accaacactc accgagcgcg catcgtcgca cacgagccac gcacccgggc
      661 acgtcgcgtg cgcggcccaa ggcaaccggc gcaggaattc gtggatttct tcgcgctcga
      721 caacacgcgg gcgacgctga cgctggtgcc ggcaagcaac gtcacgatgc agggcttcca
      781 tgcgcgcttc aagctccgcc tcggcgacgg ccgcgaggag ctgcggatcg tcgtcggcaa
      841 ccaggatgcg cgcatgggag agcacgccac ggcgcaatgc acggcgcgag tcgcgcggag
      901 gtttccactg attacggtga tattccaatc aggtcgagga ccggcgcgcc gaatcgcgtg
      961 cagatcttgc gctggcgggc agtcgcggca caatcgcaag cgcggcctcc tccggtgcag
     1021 attcccagtt tcgggaggtt cgccgcgcgg cgaggaatgc ctcgaacgcg caattcgcgc
     1081 cgccctcgct ccccatgcgg cgagaatcgc gcacctgcgc atttcgcgcc tcctcgttcg
     1141 attcgcaact catcgaattc accgcagatc cgggaatact gacgaatctg tcccggcgcc
     1201 cgcgtggcgt ctcctttgca ccatctaggg ccgccacgtt ccaggaggat gtcatgtcat
     1261 tgctgcgccg accgatcgag cacgagctcc accagcatca tttcccgaac aggggggcgc
     1321 cgccttcgcc cgggctggtg aagacgccgt ccacggacac gatgtggttc gagaacgcgc
     1381 accacgatca ccgccacgag caccccgggc cgttccccta cgggctggtc cagaagccgt
     1441 cgccggagac ggtgacgcac acgttccgcc acctcgtgcg cctcgacggc aagaacaaca
     1501 cgctgccggc gcccgcggag gtcatcgtcc gcacccgcgt cgagcgcacc gccggcgagt
     1561 acgtgctcgg cgacctcgtc tacacgctga gcctgctccc cggcgaggag gtgcaggtct
     1621 acacctccga tcgccgcacc agcttcaagc tcgacacgca gaccaacgtc gcgtggcagg
     1681 cgtcggcctc cttcgaggac cagatgtacc tgtcggcgat ggaccgctac ttcgcccagc
     1741 tctcggccaa cgagtgggcg caggccacca gcagcaccag cggatcgttc aacgccagcg
     1801 ccgagacgag cagcgcgctg gagagcttct tcggcggcgg cgacgccgac atggagggct
     1861 cgttcaacgt caactcggtg gcgacgttcc tcggcgggat ggcggcgcag gcgtccgccg
     1921 cccacgagtc gtcggcgacg gccacgaaca ccgccagcgc gctgaccgtc ggcgccgtcg
     1981 gcacgcgcac cggcgtgcag ggcaagatcg acacccacgt ggagatgaac taccgctcgt
     2041 tccgcaaccc gagccggacg cgcgcggtga cgttctactt cttccagatc cggcgccggc
     2101 acaaggtccg cgtcaccgtc gacaagctcg aggtggcggc gcccgagcgc acgctttggc
     2161 gcatggcgct cgcggacgcg aagcagaccg tgaaggcagc cccgctgatg atgcccgtcc
     2221 tcggcacgaa gcacatcgcg aacatcgcgg actaccaggc cgcgcgtgag accatcgtca
     2281 acgagatggt ggcgaagaag ctgctcgagc agaaggacgg ccagctcgtc gtccccgagg
     2341 cgctgaggac gcagacgacc ttcgagcgcg agttcacgct ctgcacgccg ggcatctacg
     2401 tgatgggcga tcgcgaccgc acgacgatcg cggacccgtc gctcctgcgc gacgcgtgga
     2461 ccgtcggcgc ggcgatcgcg gacgaggtgg aggcgctggc gatctcggcg gtggagcgcg
     2521 cgggcaacac gatcgtgccg gcggtggcgg gcgtcgtgcg aggcgcgcag gcgatgctcg
     2581 cgggggcgag ctcgctgctg ccctgaatcg atcaacgcaa gtcgatgatc ggcgcgcgct
     2641 gacgttcggg tcgagggggc gaggggggcg ccatgctgca gcaatggccg ctggccatgg
     2701 cactcacgct cccaggttgt agacgacgcc cgaaagctgc gaggcgcgcg ccgaggcgag
     2761 gaagcacacc atgtcggcga cctgctccgg ctcgatcacg tgccccatca gctcctgatg
     2821 gcggctctcg tcgccgaacc gggctttcgc ctgcgccttc atgagcgtga tgatgcggtc
     2881 ggtgcgtgtc gccggcggat gcacggccac cacgcgtacg ccgtgctcgc gcgagccttt
     2941 gcccagggcg cgggtgaagt tcgcgagccc ggcgttcgcc gcggccccgc acacgtactc
     3001 gaacgacggt tgctcggccg ccatgccgat cacgttgaca atgaccccgc tgcgtcgcgc
     3061 cttcatgcgc ggatacagct cgcgcgtgag gttgatgaag ccgaacacct tgagctccca
     3121 ggcgtgccgc cacttcgcgt cgtcgatcat ctcgatggtg cccccgggga tgtcgccggc
     3181 gttgttgacg aggatgtcca cgtcggcgca ctcggccgcc agcttgcgaa gcgcgcccgg
     3241 gtcgcgcaga tcgcacggat ggaggatcac atcgccgccc aggtcgctct ttgcccgatt
     3301 cagacggtcc ccgtcgcgcg cggcgaggtg caggcgcgcg ccttccgccg cgaagctccc
     3361 ggcgcacgca agcccgatgc ctttcgagcc accggtgatc agaactgatt tacccttgag
     3421 gccaaggtcc atttcgattc ccttttagag aagagtttgc ataccagatt ctttgtcgtg
     3481 tgcaggtgct ggatcaaggc cgtgccgccg caggacgcgc ttgaccgtgt agcgtccgac
     3541 ctggtggccg aggttgtcca gcgccccgac caaccgcgtg tagcgcccag gccggattct
     3601 ccaccgcacg ccgccgagct cgcgcatcag gtccgcgcgg acccgggcat tccgttgggt
     3661 tacagcagcc ggacgatcag cccggtgcgt acagagaggt tgcgatccag gtacacgtcg
     3721 ccggccaccc atccgcccgt cttggcaagg cgtagcctgc ttggttcgac cacgaccagg
     3781 ggcacgccgg cgagtcgggc ggcgggcgaa ggacatcacg tagcacgcgt gtgctatctg
     3841 tcggcccgga ggtcacgatg atcgagaacc tgcgcgcggg tgcctgcgtc gccacgttgg
     3901 gattcctgtt gttcaccgcc tgctcgtcga ccgctccggc gaaaggcaag gcatgggaat
     3961 cgatggaccg ctctacactc gaccgagcgt acaacaactc tgccgcggtt cccgagggcg
     4021 ctcagatgtt caaggagtgg caggcgcgca gcgcgcaggt ccgcgagcag caccccgagc
     4081 acctcgacct cccctacggc ccgcgcgagc gcaaccgcat cgactacttc tccgcgggcc
     4141 cgaacacgcc ggtcttgatc ttcttccatg gcggctactg gcagatgcgg tcgaaggccg
     4201 acttcgcttt tctcgccgag agcttcctcg gccagggcat cagcgtggcg atggtcggct
     4261 atcccctcgg cccggaggcg accatggacg agatcgtcgc cgacgcgcac gccgccgtcc
     4321 gctatctcgc ggcgcagctt ccatcgctgg gcggcgatcc ggcgcgggtg gtggtgagcg
     4381 gctggtcctc tggcgggcat ctcgcaacct cggtgcttga cgagccgtcg ctgcgcggcg
     4441 gggtggccat cagcgggctg tacgaccttg cgccgctcgt gaagtcttac gtcaacgaca
     4501 agctgcacat ggacgaggca gctgcgcggc gcaactcgcc gatgctcaac ctgccaccga
     4561 gctcgaaacc gctggagctc ttcgccgggt ccgccgagtt gccggagatg cgccgccaga
     4621 ccgccgacta cgcgagcgcc cggcgcatgg ctggcctgcc ggtgcactac gaggaaatct
     4681 cgggcgcgaa ccactacacc atcttgaacg acatgctccg cccggagggc cggatccacc
     4741 aggcgttggt ggtgatggtc ggcatcacgc cctcggtgtc ccccccgcct gaaaccaggt
     4801 aacccggcag tgcttcgatc agaacggccc cgtccctggg aggttcaacc atgccgcgtg
     4861 ctgcgcgcgg cgcggcgcgg cgcggcgcgg tgggcctggg tcaaccgaac acaggggcca
     4921 cggtcgtctg ctgacccggc tcgtcgcgcg ggtcgggtcc cgacgagcga aatgtttact
     4981 ccaaggaccg cgaagccttg gaaaatcgac aggttccgct cgggatgaca ccttggtctc
     5041 cgcgctggtc tgcttccggc cgccggtatc gcgcatgacg ctggggggac cggccggcct
     5101 gctgcacgac gtgcgtcgcc tcgtgcgcga gcaactgcgc gtcgctcatg ctcgcgctgt
     5161 ccccgagcac gatctgcgaa ccgaaggtga acacgcgcgc gtcgacatcc cgggcgatct
     5221 gcgacgccgc cgggcccgcg tgcacggtcg cggacgacag gtcaccaccg gtcccggcct
     5281 cgaccccggc ccgcacgtcg gccggcaccg gcgtcccgct ccccatcttc gccatcgcgg
     5341 tctcgaccag cgtgtgcttg ccgggcgagc tctccgcacc ggtgttgcca gccgttgcgc
     5401 cgacgagcgc gtgcttgccg accgcgggtg ccgggcctgc ggccgcaccc tcgccggcgc
     5461 tgccctcctg tccgtgcttg cgaccgaagc tactcatcgt gctcatggta ccgcgaccgt
     5521 tctgcgctgg catcacccgg tggaaacgcg atcacgttac ccctcgcgcc acgcgtccca
     5581 agcgcggatg tagtcctcga tcgagagtga gaacctgtag ccgaggctgg ccagcggccc
     5641 gactcggcgc cgaccggtcc cagccgctgg ctactcaccg ccatcaccgc cgcaaattgc
     5701 gccaattaga atcgcttgct gatccagcgc agcatagcct ccggcgttga tcacgacagg
     5761 cgcgccggcg acagcggcca cggccattcg cagaacctcg agctcgacgc cgggaaaccg
     5821 gcccacgcgg ttgaagcccg agtcccatgt gcagcccctg gtagagcccg gccgtgatcc
     5881 acagcgactg ctccatgctg gggaacgacc caccgttcgc cgccacgcag ggggcatcga
     5941 ggacgggtag ctccacgtag atgccagggc cggccaccgt cggcacaacg gtcgatccgg
     6001 gcggcgatgg cgccgaagcc gacggaacgg gaacgtcgtc cggtctgtct tcccccgcgg
     6061 ctgcaggagc ggccggcagc gcgagcggcc agcccaccgt gagcaccacg aggacagcga
     6121 ccgtggaccg gcggttcgcc aggtggcgat cgatcttgcg cttgtggctc acgaggatgc
     6181 gccttgctgg ttgctcctcg acgcacagca agcggtgttc ttccgtcgag ccgggctggt
     6241 tctacgcagc tgcgccatgt gccgcgtctc ccacggtggc aaggcgcagc ccggggacgg
     6301 cggtgctggc cgccgcggtc gacctcgccg gcgtgctcct ggtcgagctc gaccgccgcc
     6361 ccacgccgcc cacccgccac cagcacgccg cacggccgtg ccgcgtcccg ctcctcaatc
     6421 gcccgagctc agtggcaggc ggcgacggtg tactgcgtgt acgaccggct ggtggccgga
     6481 tcgatggcgc tgctgctggt cacgcactgg ccggcgctgg cggtgaagcc gtggcccgcc
     6541 gggctggtgg cgataccgaa cgagccggtc gcgacctcga tctggttgct cgcatcgctg
     6601 gtgcggacgc ttccgtcgag cagcacctgc gtgacgtcgt agaccggcgg ggtgtcgccg
     6661 agcacggtgt gatcgacgct gagctcgagg tcgatcttcg gcgtgacggt gatcgtctcc
     6721 ttgccggtcg tggggtcgcg gctcaccgtc gcatcgaatg cgcggccgtc gtgcgggttg
     6781 atgtcgatgg tctcggcgcg ggcgccgtcg agagacaccg tcgtggtgcg gttgcc
//
LOCUS       DASVOR010002110         5420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_80982,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002110 DASVOR010000000
VERSION     DASVOR010002110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5420
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80982"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3956
                     /locus_tag="VF516_39130"
     CDS             <1..3956
                     /locus_tag="VF516_39130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113830.1"
                     /translation="RHGVDPAEHDADRAAIAVVHQLHGGPGAEPAERIAPTHSAEFQL
                     QRCEGTLNEGEQEMLGFYRQGFTAHGDPFELQLSRHAPKGPVDRPLLDIAIRYAGRDD
                     ADNPTLRGVLLLPPGKSPRDPKITTDKPTDWTDMKLDVFGDASWIGGIHHSVVELPGW
                     SPRSREHTFSFTGPRDQSPRQVVVVKSAGALPIDAAQTHADPTHETGPLLRSDVAVAA
                     AKLTLDKLLTKQEFALDARKPPWPMLKAKVDAAAGKPAGDSDDARRLTRLQETLSHVR
                     PVFLALGAASNPDAYLPDISATALRFVAEVRQTYEAALAGAWQGPVDLGTADAAFNAL
                     WHRLTALYLQKGKGVDAMVRSGQNLAQDIVDLTGSGERPFDRIRNQLGVGKGGTNVLH
                     TAEHAEIVRREFTAGAKGSLAKVSAVVEDAQVITGLGTLIVTDQLFHKFKAELAGVMG
                     KIVDKTPGTRNLTRVCDERIAAIEALAQTVEDALAGARTQDELAAALRTAGRAAVGKF
                     QALANTKDYKQDLQDIQSRIKTVKLLETLGKVLAIMGVAALTGGAAGAAVGTALEGMG
                     ASAGVAATGEFAAEVAAFTLTSRLGNDLAFGKNETSLGEDLLTNAMMMGTMKAAAAVY
                     GKVFKLVADPKKYKTAYAVGGAMTGFVALQMFAEVHTLVKTGKVMDWDDRMRGLLSNA
                     LMMSAMALGGYLVRVARTNPTMHDLAAKNVGPALATLDAQIAQLAPQLEQIRAGGPKA
                     ADLAADLAPRLQELWQRQLEVFADAVRAERDPAAQARARETFKATTEAFNRELARIDL
                     QLASAGIDVQMAPGAGAGSRLFEPIKPGFVKFKPGGKQVIDDFYSHNGGRVEQVPGQD
                     GLYRGTDASGETFFVEETRADRLLDDGKHTGSLKGKDVYPENTYIDGEGKEQPAKLNE
                     KKIKDAVTGSKKAPPRGTKLDVVSVSGKTAQLKMEVATAGATAEVTVNLTVKPRDSLA
                     ASSSHGEDAGPARLDLTRGADGKWTAIVEVSETMRPEDVQFAIDHELVEAAELIRRYP
                     GGGPKGGFGKEMQSGVMKPGATTDHATAHDVAAAQEVVGLWNDHKNLVKNGSPRAAAR
                     KQTLDRAMEAQGLNDTTQVPAKIDLLRRAGAPEEMLVAIKQREAQRVFDEHVANDLGG
                     KRASSFTPALVEHLLFPEPKESTGISGVNGGHHTIELVNYADAPSNFSIGQKGTPKIH
                     GGTTYRHFEQLGWKGDPAQKPRYGTPEAPGGARFDASKWNPLGDPKTTFDDPAAFIAD
                     AEQGFEMWQQTAGSSVPNKKIKFTTPGGVEVIGYLESKTAPYKLLSIFPDSAWIP"
     gene            3983..5086
                     /locus_tag="VF516_39135"
     CDS             3983..5086
                     /locus_tag="VF516_39135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113831.1"
                     /translation="MDFPLEANQFGVGLAPSVEQLEALRYAIDEVVNGDSRDVPIETL
                     AREGFRVQGCWAVPIPPTKSPTSLAIDELHWRRVSGAWADPSVSFEVATSFTKRDVAV
                     PREVLIQLVIRLRALRDEILAGKREGRIDDTPVPPPRQLTPELEEYERLRTFSRADAD
                     AYAAELYAHRAEQAVGEQIARPDQTGSSSMQVDDDTLREIAALADDVDRLDAEASRSD
                     EPYADEQLDARLVAKRSRLLLELEVAGVLGSAERGRLERLGLAKLVAYSDAAVAMDHY
                     LDSPGYARLVKQPRVTRLVDSCVSLDWFRVPSGYVPDGVAPDDWLALCEANFRANERP
                     RAGSVFVRLEGKFHRLMFRTELDKPRLYRAVLA"
     gene            complement(5102..>5420)
                     /locus_tag="VF516_39140"
     CDS             complement(5102..>5420)
                     /locus_tag="VF516_39140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113832.1"
                     /translation="ATGLAAAAAVLIALHDRRAVRQLSHLHELDRVCRAALADGARHD
                     TSLGVAVSVHPLPDGRRDWVLSSADPRWSPAAARRIADALWADHELHEGHLAGVVHVI
                     TAG"
BASE COUNT          947 a         1838 c         1885 g          750 t
ORIGIN      
        1 cgcgtcatgg tgtcgacccc gccgagcacg acgccgatcg cgcggcgatc gcggtcgtcc
       61 accagctgca cggcggcccc ggcgccgagc cggccgagcg catcgcgccg acccacagcg
      121 ccgagttcca gctccagcgc tgcgagggca ccctcaacga gggcgagcag gagatgctcg
      181 ggttctaccg ccagggcttc acggcgcacg gcgatccgtt cgagctccag ctcagccgcc
      241 atgcgccgaa ggggccggtg gatcggccgt tgctcgacat cgcgatccgt tatgccggcc
      301 gcgacgacgc cgacaatccg acgctgcgcg gcgtgctgct gttgccgccc ggcaagtctc
      361 cgcgcgatcc gaagatcacc accgacaagc cgaccgactg gaccgacatg aagctcgacg
      421 tgttcggcga cgcgagctgg atcggcggga tccaccacag cgtcgtcgag ctgccgggct
      481 ggagcccgcg atcgcgcgag cacacgttca gcttcaccgg cccgcgcgat cagagcccgc
      541 gccaggtcgt cgtcgtcaag tcggccggcg ccttgccgat cgacgccgcg cagacccacg
      601 ccgaccccac gcacgagacc ggcccgctgc tcaggagcga cgtcgcggtc gccgccgcca
      661 agctcacgct cgacaagctg ctgaccaagc aagagtttgc gctcgatgcc cgcaagccac
      721 cgtggcccat gctcaaggcg aaggtcgacg cagcggccgg caagcccgcg ggtgactccg
      781 acgacgcgcg gcggctcacc cggctgcagg agaccctgtc gcacgtccgc ccggtgttcc
      841 tcgcgctcgg cgcggcgagc aaccccgacg cctacctccc cgacatctcc gccaccgcgc
      901 tgcggttcgt cgccgaggtt cgccagacct acgaggccgc gctcgccggc gcgtggcagg
      961 gcccagtcga tctcggcacc gccgacgccg cgttcaacgc gctgtggcac cggttgaccg
     1021 cgctctatct ccagaagggc aagggcgtcg acgcgatggt ccggagcggc cagaacctgg
     1081 cccaggacat cgtcgacttg accggctccg gagagcggcc gttcgaccgg atccgcaacc
     1141 agctcggcgt cggcaagggc ggcaccaacg tcctgcacac ggccgagcac gccgagatcg
     1201 tccgccgcga gttcaccgcc ggcgccaagg gttcgctcgc caaggtgtcc gccgtcgtcg
     1261 aggacgccca ggtcatcacc ggcctcggta ccttgatcgt caccgatcag ctgttccaca
     1321 agttcaaggc cgagctcgcc ggcgtcatgg gcaagatcgt cgacaagacc cccggtaccc
     1381 gcaacctgac ccgggtgtgc gacgagcgga tcgcggcgat cgaggcgctg gcgcagaccg
     1441 tcgaggacgc gctcgcaggt gcccggaccc aggacgagct cgccgccgcg ctgcgcaccg
     1501 ccggccgcgc cgcggtcggc aagtttcagg cgctcgccaa caccaaggac tacaagcagg
     1561 acctccagga catccagtcg cggatcaaga ccgtcaagct gctcgagacc ctcggcaagg
     1621 tcctcgcgat catgggcgtg gcggcgctca ccggcggcgc ggccggcgcc gcggtcggca
     1681 ccgcactcga gggcatgggc gcgagcgctg gcgtggcagc gaccggcgag ttcgccgccg
     1741 aggtcgccgc gttcaccctg acctcgcggc tcggcaacga cctcgcgttc ggcaagaacg
     1801 agacctcgct cggcgaggac ctcctgacca acgcgatgat gatgggcacg atgaaggcgg
     1861 ccgcggccgt ctacggcaag gtcttcaagc tggtcgccga cccgaagaag tacaagaccg
     1921 cctacgccgt cggcggcgcg atgacaggct tcgtcgcgct ccagatgttc gccgaggtcc
     1981 acacgctggt gaagaccggc aaggtcatgg actgggacga ccgcatgcgc ggcctgctgt
     2041 ccaacgcgct gatgatgtcg gcgatggcgc tcggcggtta cctggtccgg gtggccagga
     2101 ccaaccccac gatgcatgac ctcgcggcga agaacgtcgg ccccgcgctc gccacgctcg
     2161 atgcccagat cgctcagctc gcgccgcagc tcgagcagat ccgcgccggc ggccccaagg
     2221 ccgccgacct cgcggccgac ctcgccccca ggctacagga gctgtggcag cgccagctcg
     2281 aggtgttcgc cgacgcggtc agggccgagc gggacccggc ggcgcaggcc cgcgcgcgag
     2341 agacgttcaa ggcgaccacc gaggcgttca accgcgagct cgcccggatc gatctccagc
     2401 tcgcctcggc cggcatcgac gtccagatgg cgccgggcgc cggcgccggc tcccgcctgt
     2461 tcgagccgat caagcccggc ttcgtcaagt tcaagcccgg cggcaagcag gtcatcgacg
     2521 acttctatag ccacaacggc ggtcgggtcg agcaggtccc cggccaggac ggcctgtacc
     2581 gcggcaccga cgccagcggc gagacgttct tcgtcgagga gaccagggcc gatcggctgc
     2641 tcgacgacgg caagcacacc ggctcgctca agggcaagga cgtttacccg gagaacacgt
     2701 acatcgacgg cgagggcaag gagcagccgg ccaagctcaa cgagaagaag atcaaggatg
     2761 cggtcaccgg ctcgaagaag gccccgccgc gcgggaccaa gctcgacgtg gtcagcgtgt
     2821 ccggcaagac cgcgcagctc aagatggagg tggcgaccgc cggcgcgacc gccgaggtca
     2881 cggtcaacct tacggtcaag ccgcgcgaca gcctggcggc gtcgtcgtcg cacggcgagg
     2941 atgccggccc ggcgcgcctc gacctgacgc gcggcgccga tggcaagtgg accgcgatcg
     3001 tcgaggtttc tgagacgatg cgccccgagg atgtccagtt cgcgatcgac cacgagctgg
     3061 tcgaggccgc cgagctgatc cggcgctacc ccggcggtgg gcccaagggc ggctttggca
     3121 aggagatgca gtctggcgtg atgaagccgg gcgccacgac cgatcacgca accgcgcacg
     3181 acgtcgccgc agcccaggag gttgtgggcc tgtggaacga ccacaagaac ctggtcaaga
     3241 acggctcccc cagagccgcc gcgcgcaagc agaccctcga cagggcgatg gaggcgcagg
     3301 ggctcaatga cacaacgcag gtgccggcga agatcgacct gctgcggcgg gccggcgcgc
     3361 ctgaggaaat gctggtcgcg atcaagcagc gcgaagccca gcgtgtgttc gacgagcatg
     3421 tcgccaacga tctgggtggc aagcgagcgt catcgtttac gcccgcgctg gtcgagcacc
     3481 tgctgttccc ggagccaaag gaaagcaccg gcatttcggg tgttaacggc ggtcatcaca
     3541 cgatcgaact cgtcaactat gctgatgcgc ctagcaactt ctccatcgga cagaagggga
     3601 cgcccaagat ccatggcggt acgacgtacc gtcacttcga gcagcttggg tggaaaggtg
     3661 accctgctca gaagcctcgc tacgggacgc cggaggcacc gggcggagcc agatttgacg
     3721 cctcaaagtg gaacccgctc ggtgatccga agaccacgtt cgatgatcct gcagcgttca
     3781 tcgctgatgc ggaacaaggc ttcgagatgt ggcagcaaac ggcaggatca agtgtgccta
     3841 acaaaaaaat caagttcacg acccccggtg gtgtagaagt tattggatac ttggagtcaa
     3901 agactgcacc gtacaagctt ctctcgatct tcccggattc tgcatggatt ccttagtccc
     3961 tagcttcgtc tcgattggtg acgtggactt cccgctggaa gcgaaccagt tcggcgttgg
     4021 gctcgcgccg agcgttgagc agctggaagc gctgcgctac gcgatcgacg aggtggtaaa
     4081 cggtgattcg cgggacgttc cgatcgaaac cctcgcgcgg gaggggttcc gcgtccaggg
     4141 ctgttgggca gttccgatcc cgccgacgaa gtcaccgacc agtctggcca tcgacgagct
     4201 gcattggcga cgcgtgagcg gcgcgtgggc tgatccgtcg gtttcgtttg aagttgcaac
     4261 cagctttacc aagcgggatg ttgccgtgcc gagggaggtg ctgatccagc tggttatacg
     4321 gcttcgtgcg ctgcgtgacg agatcctcgc gggcaagcgc gagggccgga ttgacgacac
     4381 gccagtgcca ccgccgcggc agctcacgcc ggagctcgag gagtacgagc gactgcgcac
     4441 gttctcacgc gcggacgccg atgcatacgc cgccgagctg tatgctcacc gagcagagca
     4501 ggccgtggga gaacagatag ctcgtccaga ccaaaccggg agtagctcca tgcaagtcga
     4561 cgatgacacc ctgcgcgaga tcgcggcgct ggccgacgat gtcgaccggc tcgatgccga
     4621 ggcgagccgg agcgacgagc cgtacgccga cgagcagctc gatgccaggc tggtggcgaa
     4681 gcgcagccgg ctgctgctgg agctggaggt cgcgggcgtg ctcgggtccg ccgagcgcgg
     4741 ccggctggaa cggctcgggc tggccaagct ggtcgcgtac agcgacgcgg cggtggcgat
     4801 ggatcactac ctcgactcgc cggggtatgc gcggctggtg aagcagccgc gggtgacgcg
     4861 gctggtggac tcgtgcgtat cgctcgactg gttccgcgtg ccgtcggggt acgtaccgga
     4921 tggcgtcgcg cccgacgact ggctcgcgct gtgcgaggcg aacttccgcg ccaacgagcg
     4981 gccgcgcgcc gggagcgtgt tcgtgcgact ggagggcaag ttccaccggc tgatgttccg
     5041 caccgagctc gacaagccgc ggctgtatcg cgctgtactc gcatagcggt cccgtcatgc
     5101 gtcaaccggc ggtgatcacg tggacgacgc cggccaggtg gccctcgtgg agctcgtgat
     5161 cggcccacag cgcatcggcg atgcggcggg cggcggcggg cgaccagcgc ggatcggcgg
     5221 acgacagcac ccagtcacgg cggccgtcgg ggaggggatg gacgctgacc gcgacgccga
     5281 gcgaggtgtc gtggcgcgcg ccatcggcga gggcagcgcg gcagacgcgg tcgagctcgt
     5341 gcaggtggga cagctggcgc accgcgcggc ggtcgtgcag ggcgatcagc acggcggcgg
     5401 cggcggcgag gccggtggcg
//
LOCUS       DASVOR010002111         1754 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_256804,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002111 DASVOR010000000
VERSION     DASVOR010002111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256804"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1183)
                     /locus_tag="VF516_39145"
     CDS             complement(<1..1183)
                     /locus_tag="VF516_39145"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lantibiotic dehydratase"
                     /protein_id="HEX8113833.1"
                     /translation="MKPLHLSGATPPAAATPSGLFVMRTPLLPYDVLAAFGGALEASG
                     TTDPSRLADALARDRARLRIRLREIVARPEVREAIFLASPGLEARLGVWERDPEGRRG
                     PQIERAVFCYVARMAGRPTPFGLFATASIGAIGDHTDLMLDELTSCRRHTRLGIGYLA
                     ALAGEIDHRREVRERVHVRVNPTLYRAAGRVRYTAVQRTATSRAYRLVAADVTPYLEA
                     TVARAAQGARIADLARALVDDDPEIALEDAEGYVHELVDAQLLVSSIGPRVTGGEPTS
                     DLLDRARAHPELTGMAARLGDARDRLTAIDGGGLGAAPDRYREIARALDGPSAAAALP
                     SLFHVELARGARGMTLGREPVTEILRGVEILRRLSRPSRSLRRFVEAFAARYGDAEVP
                     LL"
     gene            complement(1272..>1754)
                     /locus_tag="VF516_39150"
     CDS             complement(1272..>1754)
                     /locus_tag="VF516_39150"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX8113834.1"
                     /translation="APGAAQTVIDLGAYRLGVAICYDAEFPLVVRRLVAAGADVIAVP
                     SCTDTPAGYHRVRVACQARALENQCYVVQAPTVGVAPWSIAVDENVGAAGVYAPPDRG
                     FPDDGVIAQGTYCAPGWVVADVDLDAIARVRADGQVLGHRDWDLPGHLAAPVGRAQLA
                     "
BASE COUNT          243 a          658 c          653 g          200 t
ORIGIN      
        1 cgagcagcgg cacctcggcg tcgccgtagc gggccgcgaa cgcctccacg aaccggcgca
       61 ggctgcgcga tgggcgcgac aggcgacgca ggatctccac gccgcgcagg atctcggtca
      121 cgggctcgcg cccgagcgtc atgccgcggg cgcctctcgc gagctcgacg tgaaacagcg
      181 atggcagcgc tgcggcggcg gacggaccgt cgagcgcccg agcgatctcg cggtagcgat
      241 cgggagcggc gccgaggccg ccgccatcga tcgccgtcag ccggtcccgg gcgtcgccca
      301 ggcgcgcggc catgccggtc agctccggat gcgcgcgagc ccggtcgagc aggtcgctcg
      361 tcggctcgcc tccggtgacc cgcgggccga tcgacgacac gagcagctgg gcgtcgacga
      421 gctcgtgcac gtagccctcg gcgtcctcga gcgcgatctc ggggtcgtcg tcgaccagtg
      481 cccgcgcgag gtcggcgatg cgcgccccct gggccgcgcg cgcgacggtc gcttcgaggt
      541 agggcgtgac gtccgccgcc accaggcggt atgcacgcga cgtcgccgtg cgctgcaccg
      601 ccgtgtagcg aacccgtcca gcggcgcggt acaaggtggg attgacccgg acgtgcacgc
      661 gctcgcgcac ctcgcgccgg tggtcgatct cgccggccag cgcggccagg tagccgatgc
      721 cgagccgggt gtggcggcgg caagacgtca gctcgtcgag catcagatcg gtgtgatcgc
      781 cgatcgcgcc gatggaggca gtcgcgaaca gcccgaacgg cgtggggcga cccgccatgc
      841 gcgcgacgta gcagaacacc gcgcgctcga tctgcggacc gcgccgcccc tccgggtcgc
      901 gctcccacac ccccaggcgg gcctcgagcc cgggcgaggc gagaaagatc gcctctcgca
      961 cctcgggccg cgccacgatc tcgcgcaggc ggatccgcag ccgcgcacga tccctggcca
     1021 gcgcgtccgc gagccgggaa ggatccgtgg tgccgctggc ctccagcgcg ccgccgaacg
     1081 ccgccagcac gtcgtacggc agcagcggcg tacgcatcac gaacaagccc gatggggtcg
     1141 cagccgcggg cggcgtggcg ccggagaggt gcagcggttt catcggaacg gtgcgccccc
     1201 gagaatatca ctcccgcccg atcggccggc cgcgcacgcc cggagtcgcc cgcatgcgcc
     1261 ggtctgcgcg atcacgcgag ctgcgcgcgc ccgaccgggg cggcgaggtg gcccggcaga
     1321 tcccagtcgc ggtggccgag cacctggccg tcggcgcgga ccctggcgat cgcgtcgaga
     1381 tcgacgtcgg cgaccaccca gccgggtgca cagtacgtgc cctgcgcgat cacgccgtcg
     1441 tcggggaagc cgcggtcggg cggcgcgtac acgccggcgg ccccgacgtt ctcgtcgacc
     1501 gcgatcgacc acggcgcgac tccgaccgtc ggtgcctgga ccacgtagca ctggttctcg
     1561 agcgcgcgcg cctgacaggc gacgcggacg cggtgatagc cggccggcgt gtccgtgcag
     1621 ctcggcaccg cgatgacgtc ggcgcccgcc gcgaccagcc ggcgcaccac gagcgggaac
     1681 tcggcgtcgt agcagatcgc gacgccgagc cggtacgccc cgagatcgat cacggtctgc
     1741 gcggcgccgg gtgc
//
LOCUS       DASVOR010002112         5516 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_111058,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002112 DASVOR010000000
VERSION     DASVOR010002112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111058"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="VF516_39155"
     CDS             <1..1648
                     /locus_tag="VF516_39155"
                     /inference="COORDINATES: protein motif:HMM:NF017411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113835.1"
                     /translation="RISTVVDVTFGKTLTYAYDANGNRVSLTEGATTFGYAYDAMNRL
                     KQADAFGGSIFFEYDAAGRRTSLLRPNGIRTTWAYDNGIQVASITHAKGDNVVLGFTY
                     EYDFHGMRASKTREDGTKEVYGYDAADRLVQVDYGSSRRVQYGIDPLGNRVLEDTTVP
                     GQPLVRTIGTFNGFNQLMTTGGPGSTIPLTDFAYDLNGNLTSETRHPTGLPDEVTAYG
                     WDRDNRLRSVSPPLGTPTTYRYDANGLRTERVDMTGTTRYLLDGPTVLEELDSTNAAS
                     VRYFSNPQRIDEVLSYQRPGSVEYPLTDALGSIHVVSDDQGNIVHRYDFGVFGERTDL
                     GGSTAAIDAGYTGRWHDPNGLLEHRDRQRNPKLGGWLQPDRLNTAVEGAQAARTAASL
                     MPAEAWRFTADIVRDVSNVDTNLYAYALNNPLYWMDPNGFAVYNYSSRTIWAKPEKED
                     GNGDIPFPVLPHSKSLDEQDGIADPLYPGWVYKSIDWVDIIVYDSFGVAPWSLFPLWA
                     GIPQFWGGGWKDEQWLQELHKKGDHGWDKLFEKSRSGPCR"
     gene            1660..1809
                     /locus_tag="VF516_39160"
     CDS             1660..1809
                     /locus_tag="VF516_39160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113836.1"
                     /translation="MSRTEGWKAVTLRRHLFGPTDRMPDYLVLPRETSAADRRRAVTV
                     GNDGR"
     gene            complement(2062..2580)
                     /locus_tag="VF516_39165"
     CDS             complement(2062..2580)
                     /locus_tag="VF516_39165"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8113837.1"
                     /translation="MDKRILILAGPNGAGKTTFAREFLVGEASCPAFVNADLIAAGLS
                     PFDPARAALRAGRLMLELIAEHVARGESFAFETTLAGRNFARAIEQWRAAGYHVSLIF
                     LSLPSADLAVQRVAQRVRQGGHAVPEEDVRRRFSRGWDHFETLYKPLVDRWALYDNAG
                     DAPILIASGGTP"
     gene            complement(2882..3487)
                     /locus_tag="VF516_39170"
     CDS             complement(2882..3487)
                     /locus_tag="VF516_39170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113838.1"
                     /translation="MKRSAFSLLALPALAVLLGACGARTPSTTTGSGTGPNAETGLAA
                     FETVRSVLQHARCQNCHPRGDTPLQGDDGHLHNQNVLRGPDGHGMVGERCTTCHGPMN
                     PPDSYGEHSPPGVSTGWRMPKPEQRLVFVGVAPGALCEQIKDPARNGGKDMAALREHL
                     DDPLVTWAWTPGFGRKPPPVSRQAFLDAWTTWANAGAPCPK"
     gene            complement(3501..>5516)
                     /locus_tag="VF516_39175"
     CDS             complement(3501..>5516)
                     /locus_tag="VF516_39175"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX8113839.1"
                     /translation="NAFLRIGADDSVTVLLAHSEMGQGSWTGLALMVAEELECDWSKV
                     RCEHAPAAPVYAHTGFGVQMTGGSSATWSEFDRYRTVGATARDMLVRAAAARWKVPAP
                     SIKVASGVLSHGKNQLSFGKVADDAMKLAPPKTVQLKDPKEWKLIGTKVRRLDTPEKI
                     TGKAQFGIDVQFPELRTALVARPPAFGAKLAKFDPSAALKVPGVEKVVPTANGVAVVA
                     KHFWAAKLGRDALHIEWTPPEGGGVDTAKQMAEFHAKAKMPGDVVVEKGQKGQVETAL
                     AAAKTKLVSDYEFPYLAHAPMEPLNCAVKIDGDRVEIWTGTQFQFGDQMAAAQIVGTS
                     PDKVAIHTMFLGGGFGRRASPQADFVAEAVIVAKAAGVPVKVVWTREDDMHGGYYRPA
                     YVHRIQAGLDDKGRPVAWDHVVVGQSIMAGTPFEPMMVKHGIDPTSVEGLAESPYLES
                     TGALRVTLHSPRTAIPVLWWRSVGNTHTAFAMECTIDEAAHASGQDPLAYRLGLLKEK
                     PRHAALLQLAADKAGWSNPPPAGRARGLAVHESFGSIVAQVAEVSVDQGRIRVHRVTA
                     AVDCGLAVNPLAVEAQVQGSVAYGLSAALYGKLTFAGGAVQESNFHDYPVVRMFDMPQ
                     VDVHIMPSKAKMGGIGEPATAPIAPAVANAVFALTKQRLRSLPLKLA"
BASE COUNT          897 a         1801 c         1908 g          910 t
ORIGIN      
        1 ccgaatcagc accgtcgtgg acgtcacctt cggcaagacg ctcacgtacg cctacgacgc
       61 caacggaaat cgcgtgtcgc tcaccgaggg tgccaccacg ttcggatatg cctacgacgc
      121 gatgaaccgc ctgaagcagg cagacgcctt cggcggcagc atcttcttcg agtacgacgc
      181 cgcggggcgg aggacgtcgc tgcttcgccc caacggcatc cgcaccacct gggcctacga
      241 caacgggatc caggtggcct ccatcaccca cgcgaagggc gacaatgtcg ttctcggctt
      301 cacgtacgaa tatgactttc acggcatgcg ggcgtcgaag acgcgcgagg acgggacgaa
      361 ggaggtctac ggctacgatg ccgccgatcg gctcgtccag gtcgactacg gcagctcgcg
      421 ccgcgtgcag tacggcatcg acccgctcgg caaccgggtg ctcgaggaca ccacggtccc
      481 cggccagccg ctcgtgcgca ccatcggcac gttcaacggc ttcaaccagt tgatgacgac
      541 cggcggaccc gggagcacca ttccgctgac cgacttcgcg tatgacttga acgggaatct
      601 cacctccgag acgaggcacc cgactggact ccccgacgag gtgacggctt atggctggga
      661 cagagacaat cgactcaggt cggtcagtcc gcccctcggg acgcccacga cgtacaggta
      721 cgatgcgaac ggcctgcgca ccgagcgcgt cgacatgacc ggaaccaccc gctacctgct
      781 tgacgggccg accgtcctgg aggagctcga cagcacgaac gcggcgtccg tacggtactt
      841 cagtaacccg caacggatcg acgaggtgct ctcctatcag agacccggtt ccgtcgagta
      901 tccgctcacg gatgctctgg ggagcatcca tgtcgtcagc gacgaccagg gaaacatcgt
      961 tcatcgctat gacttcgggg tcttcggaga gcgcacggat ctcgggggta gcaccgctgc
     1021 gatcgacgct ggatacaccg gtcgctggca cgaccccaac ggactcctcg agcaccgcga
     1081 ccgccagcgc aatccgaagc ttggcggttg gctgcagccc gatcggctaa ataccgctgt
     1141 tgagggtgcg caagccgctc ggacagctgc ttccctcatg ccagcggagg cgtggcgatt
     1201 cacagccgac atcgtgcgcg atgtcagcaa tgtagatacg aatctatatg catacgcctt
     1261 gaataatccg ttatactgga tggatccgaa tgggtttgct gtttataatt attccagtcg
     1321 aacaatctgg gcgaagccgg aaaaggaaga cggaaatggg gatattccat tcccagtttt
     1381 gccacattca aaatctcttg atgaacagga tggcatcgcc gaccctttgt atccaggatg
     1441 ggtctacaag agtatcgact gggttgatat tatagtctat gactcgtttg gagtggcgcc
     1501 atggtcactg ttccctttgt gggcaggcat cccccagttt tggggtggtg gatggaagga
     1561 tgagcagtgg ctgcaagagc tgcacaaaaa aggtgaccat ggttgggaca agctatttga
     1621 aaaatcacgc agtgggccat gccggtgacg gatgccacca tgtcgcgcac cgagggctgg
     1681 aaggccgtga cgcttcgacg acacctgttc gggccgacgg accgcatgcc cgactacctc
     1741 gtgctgccgc gagaaacgtc ggcggccgat cgccggcggg cagtgaccgt cggcaatgat
     1801 ggtcgctgaa tgactgttag atggccaggc ggccggctca cggcaggtct tcccgagcag
     1861 ttactcggtc ggctcagggc aaggcgtttc cgagcagtta ctcggtcggt aactcgtgcg
     1921 gcgggatcaa gatgatctgg ccgttgcggt agaccacgag acaggtgttc gtcgcgcgag
     1981 cgacttcgat ggcgcgtagc cgcgcgcgac gaagcgcagc gagtgctccg agcgcgaatg
     2041 gcggcggggt tgcatcgatg gtcatggcgt tcctcccgaa gcgatcaaga taggcgcatc
     2101 gcctgcattg tcatacaacg cccatcgatc gacgagcggt ttgtacagcg tctcgaagtg
     2161 gtcccagccg cggctgaagc gccgccgaac gtcctcctcc ggcacggcgt gaccgccttg
     2221 ccgcacccgc tgtgcaaccc gctggaccgc gagatctgcc gacggtagcg acaggaagat
     2281 caacgaaacg tggtagcccg cagcacgcca ctgctcgatc gcacgcgcga agttccgccc
     2341 ggcgagcgtc gtctcgaagg cgaagctttc acctcgcgcg acgtgttctg cgatcaattc
     2401 cagcatcagg cgtccggcgc gcaacgccgc gcgtgctgga tcgaacggtg acaggcctgc
     2461 cgcgatcaga tcggcgttca cgaacgctgg acagctggct tccccgacga ggaactcgcg
     2521 ggcgaaggtc gtcttgccgg caccgttcgg tccggcgagg atgaggatcc gcttgtccat
     2581 tgcgttcggc gacattctac cgccggccag accattcggc gttgcggacg cggtcgacga
     2641 cctggaccag cacaaacgct gcggatttcg gctggtgcgc ctgcaacaac ccctgtcacg
     2701 cgccgaacga agccaccgag gaggtcatct gcggccgtcc gcacggacct gcggaggttg
     2761 cagaccacgc ggtggaactg gccgagtagc ccgacgaccg ctgagcttga tgacgctcaa
     2821 cgagtcgtcg cggctaccca ggacgccgcc ggaagcgccg cgacgtggct cggcgctgtt
     2881 tctacttcgg gcagggggcg ccggcgttgg cccaggtggt ccaggcgtcg aggaacgcct
     2941 ggcgcgacac cgggggcggc ttgcggccga agcccggggt ccacgcccag gtgacgagcg
     3001 gatcatcgag gtgctcgcgc agcgcggcca tgtccttgcc gccgttgcgc gccgggtcct
     3061 tgatctgctc gcacagcgcg ccgggggcga cgccgacgaa cacgagccgc tgctcgggct
     3121 tgggcatccg ccagccggtc gagacgccgg ggggcgagtg ctcgccgtag ctgtcgggcg
     3181 ggttcatcgg gccgtggcag gtggtgcagc gctcgccgac catgccgtgg ccgtcggggc
     3241 cgcgcaggac gttctggttg tgcaggtggc cgtcgtcgcc ttgcagcggc gtgtcgccgc
     3301 gcgggtggca gttctggcag cgcgcgtgct gcagcacgct gcgcacggtc tcgaacgccg
     3361 cgagcccggt ctcggcgttc gggccggtgc cggagccggt ggtggtcgac ggcgtgcgcg
     3421 cgccgcacgc cccgagcagg acggccaggg cgggcagggc gaggagagag aaggctgatc
     3481 gcttcatgga gcttcgatcc ttaggccagc ttgagcggca gcgagcgcag ccgctgcttg
     3541 gtcagcgcga acaccgcgtt ggcgacggcc ggggcgatcg gcgcggtcgc cggctcgccg
     3601 atgccgccca tcttcgcctt gctcggcatg atgtggacgt cgacctgggg catgtcgaac
     3661 atgcggacga ccgggtagtc gtggaagttg ctctcctgga cggcgccgcc ggcgaacgtc
     3721 agcttgccgt agagcgccgc ggacaggccg taggcgaccg agccctggac ctgggcctcg
     3781 acggcgagcg ggttgaccgc gaggccgcag tcgaccgcgg cggtgacgcg gtggacccgg
     3841 atccggccct ggtcgaccga gacctcggcg acctgggcga cgatgctgcc gaacgactcg
     3901 tggacggcca ggccgcgggc gcgcccggcc ggcggcgggt tgctccaccc ggccttgtcg
     3961 gcggcgagct gcagcagcgc ggcgtggcgc ggcttctcct tgagcaggcc gaggcggtag
     4021 gccagcggat cctggcccga cgcgtgggcg gcctcgtcga tcgtgcactc catcgcgaac
     4081 gcggtgtggg tgttgccgac cgagcgccac cacaggaccg ggatcgcggt cctgggcgag
     4141 tgcagcgtga cgcgcagcgc gccggtgctc tcgaggtacg gcgactcggc gaggccctcg
     4201 accgaggtcg ggtcgatgcc gtgcttgacc atcatgggct cgaacggcgt gccggccatg
     4261 atcgactggc cgaccacgac gtggtcccac gcgaccgggc gccccttgtc gtcgaggccg
     4321 gcctggatcc ggtggacgta cgccgggcgg tagtagccgc cgtgcatgtc gtcctcgcgg
     4381 gtccacacga ccttgaccgg gaccccggcg gccttggcga cgatcaccgc ctcggccacg
     4441 aagtcggcct gcgggctggc gcgccggccg aagccgccgc cgaggaacat ggtgtggatc
     4501 gcgaccttgt ccgggctggt gccgacgatc tgggccgccg ccatctggtc gccgaactgg
     4561 aactgcgtgc cggtccagat ctcgacccgg tcgccgtcga tcttcaccgc gcagttgagc
     4621 ggctccatcg gcgcgtgggc gaggtacggg aactcgtagt ccgagaccag cttggtcttg
     4681 gccgccgcga gcgcggtctc gacctggccc ttctggccct tctcgacgac gacatcgccg
     4741 ggcatcttgg ccttggcgtg gaactcggcc atctgcttgg cggtgtcgac gccgccgccc
     4801 tccggcgggg tccactcgat gtggagcgcg tcgcggccga gcttggcggc ccagaagtgc
     4861 ttggcgacca ccgcgacgcc gttggcggtc gggacgacct tctcgacgcc gggcaccttg
     4921 agcgccgcgc tcgggtcgaa cttggcgagc ttggcgccga acgcgggcgg cctggcgacc
     4981 agtgccgtgc gcagctccgg gaactggacg tcgatcccga actgcgcctt gccggtgatc
     5041 ttctcggggg tgtcgagccg ccggaccttg gtgccgatca gcttccactc cttggggtcc
     5101 ttgagctgga cggtcttggg cggcgcgagc ttcatcgcgt cgtcggcgac cttgccgaac
     5161 gacagctggt tcttgccgtg gctgagcacg ccgctcgcga ccttgatgct cggcgccggc
     5221 accttccagc gcgcggcggc ggcgcggacc agcatgtccc tggcggtcgc gccgaccgtg
     5281 cggtagcggt cgaactcgct ccaggtcgcc gacgagccgc cggtcatctg gacgccgaat
     5341 cccgtgtggg cgtagaccgg cgccgcgggc gcgtgctcgc agcggacctt cgaccagtcg
     5401 cactcgagct cctcggcgac catcagcgcc aggccggtcc agctgccctg gcccatctcc
     5461 gagtgggcga gcagcacggt caccgagtca tcggcgccga tccggaggaa cgcgtt
//
LOCUS       DASVOR010002113         3631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_52184,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002113 DASVOR010000000
VERSION     DASVOR010002113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3631
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52184"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2950)
                     /locus_tag="VF516_39180"
     CDS             complement(<1..2950)
                     /locus_tag="VF516_39180"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8113840.1"
                     /translation="MADRDLSGHTLGEFTLLEKIGQGGYGAVYRAEQPLLDRHVVVKV
                     LRKNGDVAEQRFLREAQLASRFDHPFAAHIYAFGVDEGVDLLWIAMELVQGVTLSSWI
                     EQHGPMPLEQFVPFFECIADAVHAAHECGIVHRDLKPSNVMVVERGSRLLPKLLDFGI
                     ALDQRSDAAPAEPPHPDDEASRARGATTTIRLRPASQVEHRTVTGSRGKQYRLTRTGA
                     ACGSRPYMSPEQWSNAQAAGPASDVYSLGVVAYEVLTGRLPYAAEDSAELYRKHRYAE
                     LPRLGDGFPPDLDRVIECALAKLPEHRYRSPLEMAAELREALQAQPREQLRSLARVWN
                     DRARSPALLLRGGDLLHTPTETIGELERAFVTASHRRAARLRRALVAFAVVVMMGLVL
                     GWAAMQTRMSDRVAIESDVEQGRQALLHGESIEAVRHLEQAYQRGEHSSEVAFMLARA
                     LQPRMSELAHFTSSSGRMWSAVFAPDGKRVLTSDDKSARMWDARSGQLLFTMNHGDTV
                     YQAVFSPDGSRIITAGGDGTVRIWNAASGAPIRELRYRGDVSKRWGYYAVAMSSHFVA
                     AIDVTGRTVHVWDVETGAQIAELGNDAPGIALVAFSADGHWIATAGRDEVRVFDTSTW
                     QQASKIPGPRVRSLSFDPTGPRLAVGTYDGVASIWEIPRGASVRRLRSSGASVDATAF
                     SRDGLLVATASRDGMEQVWDAASGALQTQFTSHRDKIYAVEFSRTGDLILSAGEDGAV
                     VVSNVATGMPVARLEGPKALVIAAHFDSESRRVVGASYDGTARVWEAALPYRRWNSPQ
                     IGTECDTASSLVPDQRFIAFSCRDHGTYVWDTTRGELLAELPSVTTVEGNYTSAFPAL
                     TASGDQAAIARGNTVEVYALPSGQLLRTVAHPVAVSAVAFAPAGHDLVSGAVDGSLLI
                     THDGSESIELQRSSAAIDAATILPDGRVAVVDASDRLRIIAPDHNALLMDLATPSRIR
                     LLRQSPDGKRL"
     gene            complement(3098..>3631)
                     /locus_tag="VF516_39185"
     CDS             complement(3098..>3631)
                     /locus_tag="VF516_39185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113841.1"
                     /translation="ALARRLAELHGGTLVAASEGEGRGSTFTLTLGAAAPAAAEVVAA
                     EISRIARPASNGKLAIVVIEDNDDAADTLAEWLETMGHSVRVARTGPEGVDLVREHRP
                     RLVLCDIGLPGMDGVEVCRRVKQLPIGPQPVMVALTGWGMEADRKRTREVGFDHHLVK
                     PVAPDKLFAILNAMPPE"
BASE COUNT          596 a         1230 c         1223 g          582 t
ORIGIN      
        1 cgagccgttt gccatccggt gactgccgaa gcaacctgat gcgggatggt gtggcgaggt
       61 ccatcagcag tgcgttgtgg tccggggcga tgattcgcag ccggtcactg gcatcaacca
      121 ccgccacgcg gccatcaggg agaatggtcg cagcatcgat cgctgcgctc gacctctgca
      181 gctcgatcga ctcactacca tcgtgcgtta tcaggagcga accatcgacg gcaccgctga
      241 ccaggtcgtg gcctgcaggg gcgaacgcaa ccgcactgac tgccacggga tgggcgaccg
      301 tgcgaaggag ttggccgctg gggagtgcgt agacctctac cgtgttgccg cgtgcgatcg
      361 ccgcttggtc gccggacgca gtcaacgcgg ggaacgccga ggtgtagttt ccctcgacgg
      421 tggtcacgct tggcagctcc gcaagcaact cgccacgcgt ggtgtcccag acgtaggtgc
      481 catggtctct gcagctgaac gcgatgaacc gctgatcggg tacgaggctc gacgcagtgt
      541 cgcactccgt gccgatctgc ggggaattcc agcggcgata cggcaaggcc gcctcccaca
      601 cccgtgcggt accgtcgtac gacgcaccga cgacgcggcg cgactccgaa tcaaagtgag
      661 cggcgatgac gagggccttg ggtccttcga gtctggcgac tggcatcccc gttgcgacgt
      721 ttgaaacgac cactgcgccg tcctcgccgg cagagaggat gagatcgccg gttcgggaga
      781 actcgaccgc ataaatcttg tcacgatgcg aagtgaactg cgtctgcaga gcacccgacg
      841 ctgcatccca cacctgttcc ataccatcac gactcgccgt cgcgacgagt aggccatcgc
      901 gagaaaacgc cgtggcgtca accgatgcgc cagagctgcg caagcgtcga acgctcgcac
      961 cgcgtgggat ctcccagatc gaagcaacgc cgtcataggt accaaccgca agacgcggac
     1021 cggtcggatc gaagctcagg ctgcgcaccc gaggtccagg gatcttgctc gcttgctgcc
     1081 atgtcgaggt gtcgaacacg cgcacttcat cgcgaccggc ggtcgcaatc caatgcccgt
     1141 cagcactgaa cgcgaccaag gcgattccgg gcgcgtcgtt gccgagctca gcgatctgcg
     1201 ctcccgtctc gacatcccag acgtgtacgg tccttcctgt cacatcgatc gcagccacga
     1261 agtgcgacga catcgcgacg gcgtagtagc cccagcgctt tgatacatcc ccgcggtacc
     1321 gcagctcacg gataggagct ccgcttgcgg cgttccagat ccggacggtg ccatcgcctc
     1381 cggcagtgat gatccgcgag ccgtccgggc tgaacacggc ctgatacacc gtgtcgccgt
     1441 ggttcatggt gaacaacagc tggccggacc tcgcgtccca catccgcgcg ctcttgtcgt
     1501 cgctcgtgag cacccgcttg ccgtcgggtg cgaacaccgc cgaccacatg cggcccgagc
     1561 tgctggtgaa gtgcgcgagc tcggacatgc gtggctgcaa cgcgcgcgcg agcatgaagg
     1621 cgacctccga ggagtgctcg ccgcgctgat acgcctgctc gaggtggcgc accgcttcga
     1681 tcgactcgcc gtggaggagt gcctggcggc cctgctcgac atcggactcg atcgcgactc
     1741 gatcggacat gcgcgtctgc atcgctgccc agcccaggac caggcccatc atcacgacca
     1801 cagcgaacgc cacgagggct cgccgaagcc gcgcggcgcg gcgatgactt gccgtcacga
     1861 acgcccgctc gagctcgccg atcgtctccg tgggagtgtg caagaggtcc ccaccgcgca
     1921 gcagcagcgc gggcgatcgc gcgcggtcgt tccacaccct ggccagcgat cggagctgct
     1981 cgcgcggctg cgcctgcagc gcttcacgca gctcggcggc catctccagc gggctgcgat
     2041 agcgatgttc ggggagcttg gcgagcgcgc actcgatgac ccgatcgaga tcgggcggaa
     2101 agccgtcgcc gagcctgggc agctctgcgt agcgatgttt gcggtagagc tccgcggagt
     2161 cctccgcggc gtagggcaac cgtccggtca agacctcgta ggcgacgacg cccagcgaat
     2221 agacatcgct cgccgggcca gcggcctggg cattgctcca ttgctcgggg gacatgtacg
     2281 gacgcgagcc acacgccgcg ccggtgcgag tcagccggta ctgcttgcct cgggaaccgg
     2341 tgaccgtccg atgttccacc tgggacgccg gccggagtcg tatcgtggtg gtggcgcctc
     2401 gggcgcgcga cgcttcgtca tcggggtggg ggggctccgc tggcgcggcg tccgatctct
     2461 ggtccagggc gatcccgaag tcgaggagct tggggagcaa gcggctgccg cgctccacca
     2521 ccatcacgtt ggagggcttg agatctcggt gcacgatgcc gcactcgtgg gcggcgtgca
     2581 cggcgtcggc gatgcactcg aagaacggca cgaactgttc cagcggcatc ggcccgtgct
     2641 gctcgatcca cgatgacagc gtgacgccct ggaccagctc catcgcgatc cacagcaggt
     2701 ctacgccttc gtcgacgccg aaggcgtaga tgtgagctgc aaacgggtga tcgaaccgtg
     2761 atgcgagctg ggcctcgcgg aggaatcgct gctcggcaac gtcaccgttc ttccgcagca
     2821 ccttcacgac gacgtgccgg tcgagcagcg gctgctccgc gcgatagacc gcaccgtagc
     2881 ccccctggcc gatcttctcg agcagggtga actcgcccag cgtgtgcccg gagagatcgc
     2941 gatcggccat cgacgcttgg ctcctcggtg gcgagtctag gcgcgtgaac attccccgga
     3001 tacgggcgca gccgtcgcat tttgcgtcag ccgacggcga acggtgatcc cgctgtgggc
     3061 tggggtacga ggccttgtca cccccgagga gccgagctca ctccggcggc atcgcgttga
     3121 ggatcgcgaa cagcttgtcg ggggcgaccg gcttgaccag atggtgatcg aagccgacct
     3181 cgcgggtccg cttgcggtcg gcctccatgc cccacccggt cagcgcgacc atcaccggct
     3241 gggggccgat cggcagctgc ttgacgcggc ggcagacctc gacgccgtcc atgccgggca
     3301 gcccgatgtc gcacagcacg agccgcggcc ggtgctcgcg caccaggtcg acgccctcgg
     3361 ggccggtgcg cgcgacccgg accgagtggc ccatggtctc gagccactcg gcgagggtgt
     3421 cggcggcatc gtcgttgtcc tcgatcacga cgatcgcgag cttgccgttg ctcgccgggc
     3481 gtgcgatccg gctgatctcg gcggcgacga cctcggcggc ggccggcgcg gcggcgccca
     3541 gggtcagcgt gaacgtgctg ccgcggccct cgccctcgct ggcggcgacc agcgtgccgc
     3601 catgcagctc ggcgagccgg cgggcgagcg c
//
LOCUS       DASVOR010002114         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401682,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002114 DASVOR010000000
VERSION     DASVOR010002114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401682"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..557
                     /locus_tag="VF516_39190"
     CDS             <1..557
                     /locus_tag="VF516_39190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8113842.1"
                     /translation="RPPRAGTIRFRGQSLAALPAAARAELGLALVPEGRDLWPQLTVL
                     ENLELGAYTRRARGRVRANLARMFEMFPRLDERRRQLAGSLSGGEQQMCAIARALMCE
                     PSLLMVDELSLGLAPVVVDQLMRAVGELHRGGMTILLVEQNLQKALAVAERGVIIETG
                     RLRLAGTSAELAGNPEIRAAYLGM"
     gene            624..1667
                     /locus_tag="VF516_39195"
     CDS             624..1667
                     /locus_tag="VF516_39195"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEX8113843.1"
                     /translation="MTTQNRRIVLASRPTGAPREDNFRLETVPIPDLAPGQVLVRHHY
                     LSLDPYMRGRMSDAKSYAQPQPLDEVMIGGTVGEVVATRSDRFAVGDQVLGMGGWQEY
                     AVVVADAESSTGPQRGSAIRKVDSTRIPLPAYLGAVGMPGVTAWYGLNMICEPKAGET
                     VAVSAASGAVGGVVGQLARAKGCRVVGIAGGQAKCDYVVRELGFDACIDYKAAATPED
                     LQARVREVTPKGIDGYFENVGGAVLDAVMLQMNPFGRIAVCGMIAGYNDTPTPMALPR
                     LILTQRLKIQGFIVSEHPALWPKALAELGAGVATGAIKYRESVAQGLEAAPAAFLGML
                     QGKNFGKQLVKLV"
     gene            1681..>1867
                     /locus_tag="VF516_39200"
     CDS             1681..>1867
                     /locus_tag="VF516_39200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113844.1"
                     /translation="MARIPYPDPKTLLPETQDQLAKLPPLNLFRMLAGGEGLLHAFVK
                     FGNHLWFKADLPPVLREI"
BASE COUNT          260 a          658 c          687 g          262 t
ORIGIN      
        1 tgcgcccgcc gcgcgccggt acgatccggt tccgcggcca gtcgctcgcg gcgctgccgg
       61 cggcggcccg cgccgagctc ggcctcgcgc tggtccccga gggccgcgac ctgtggccgc
      121 agctcaccgt gctggagaac ctcgagctcg gcgcgtatac ccggcgggcc cggggccgcg
      181 tgcgcgccaa cctggcccgg atgttcgaga tgttcccgcg gctcgacgag cgccgccgcc
      241 agctcgccgg cagcctgtcg ggcggcgagc agcagatgtg cgcgatcgcc cgcgcgctga
      301 tgtgcgagcc gtcgctgctg atggtcgacg agctcagcct cggcctggcg ccggtcgtgg
      361 tcgaccagct gatgcgcgcg gtcggcgagc tccaccgcgg cggcatgacg atcctgctgg
      421 tcgagcagaa tctccagaaa gcgctcgcgg tcgccgagcg cggcgtgatc atcgagaccg
      481 gccggctacg gctcgccggc accagcgccg agctcgccgg caatccggag atccgcgccg
      541 cgtacctcgg gatgtgagcg cgcgccgggc cgaccggccg gctggcgggt gtaggctgcg
      601 tggcgcagct cccaggagtc gacatgacca cccagaatcg ccgcatcgtg ctcgcgtcgc
      661 gcccgaccgg cgcgccccgg gaggacaact tccggctcga gaccgtcccg atcccggacc
      721 tcgcgccagg ccaggtgctg gtgcgccatc actacctgtc gctcgatccg tacatgcgcg
      781 gccggatgag cgacgcgaag tcgtacgcgc agccgcaacc gctcgacgag gtcatgatcg
      841 gcggcaccgt cggcgaggtc gtggcgaccc gctccgatcg gttcgcggtc ggcgatcagg
      901 tgctcggcat gggcggctgg caggagtacg ccgtggtggt cgcggacgcc gagtcctcga
      961 cagggccaca gcggggctcg gcgatccgca aggtcgacag cacgcggatc ccgctgccgg
     1021 cgtacctcgg cgcggtcggc atgcccggcg tgaccgcgtg gtacggcctg aacatgatct
     1081 gcgagcccaa ggccggcgag accgtcgcgg tcagcgcggc gtcgggcgcg gtcggcggtg
     1141 tggtcggcca gctcgccagg gccaagggct gccgcgtggt cggcatcgcc ggcggccagg
     1201 ccaagtgcga ctacgtcgtc cgcgagctcg ggttcgacgc gtgcatcgac tacaaggccg
     1261 ccgcgacgcc cgaggacctc caggccaggg tgcgcgaggt gaccccgaag ggcatcgacg
     1321 gctacttcga gaacgtcggc ggcgcggtgc tcgacgccgt gatgctgcag atgaacccgt
     1381 tcggccggat cgcggtgtgc ggcatgatcg ccggctacaa cgacacgccg accccgatgg
     1441 cgttgccccg gctgatcctg acccagcgcc tgaagatcca gggcttcatc gtgtcggagc
     1501 atccggcgct gtggcccaag gcgctcgccg agctcggcgc cggggtcgcg accggggcga
     1561 tcaagtaccg cgagtcggtg gcgcagggcc tggaggccgc gccggcggcg ttcctcggca
     1621 tgctccaggg caagaacttc ggcaagcagc tggtcaagct ggtgtgacag gggcgtagca
     1681 atggcacgta ttccgtatcc ggatcccaag acgctgttgc ccgagaccca ggatcagctc
     1741 gccaagctgc cgccgctcaa cctgttccgc atgctggcgg gcggcgaggg cctgctgcac
     1801 gcgttcgtca agttcggcaa ccacctgtgg ttcaaggccg acctgccgcc ggtgctgcgc
     1861 gagatcg
//
LOCUS       DASVOR010002115         1767 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_168405,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002115 DASVOR010000000
VERSION     DASVOR010002115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168405"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1767)
                     /locus_tag="VF516_39205"
     CDS             complement(<1..>1767)
                     /locus_tag="VF516_39205"
                     /inference="COORDINATES: protein motif:HMM:NF016615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiopeptide-type bacteriocin biosynthesis
                     protein"
                     /protein_id="HEX8113845.1"
                     /translation="ARDALLLRKVEQARRNGQHRLDLSLDELLRPGHDDALPLPAAFH
                     VVARIAARSRQALSGGDFTVLIESAAGPSGANLLGACWRGDDPLLARLSGSLRAEEAV
                     RPGAVFAELVVLPEGETGDLMVRPVLRGHEIPILARSGAPEERQIPLSDLVVCIDRAE
                     RIVVRSLRLGCEVIPRMTAPVGASSPGIGVVRFLCALQHQGTATDLAFRWGPLEALAV
                     LPRVQFGRVILARARWRLFADALHGLATPGVARFRAVQQLRAELGIPRHVEIGESERW
                     LPLDLDNALCVEVLGHAARGRRDVVLREPFPAFEELCAAGPDGRFVHELVIPFLRDNL
                     THLESEPVAPPARIARRFLPGSEWLQADLHTGLGSADRVITELVRPWVADARIRGAAD
                     RWFFLRYADHGARLRVRVHGDPARLLGEVFPALQARAAALVQHGVIWNVALDTYQRET
                     ERFGGPHGIEIAEHMFHADSDAVAEILDHLQGDAGEIARWQLTLRGIDLLLDDLGLDR
                     RAKLEVVRVGKAKLAGMLQASPGLDRQLGAAYRAQRTSLEAILDPAGDPTGDLAAPLA
                     VLHGRSTRLAPWVHELRGRERA"
BASE COUNT          265 a          656 c          640 g          206 t
ORIGIN      
        1 ccgcgcgctc gcgcccgcga agctcgtgca cccacggcgc cagcctcgtc gagcgtccgt
       61 gcagcaccgc gagcggcgca gcgaggtcgc ccgtcggatc gccggccggg tcgaggatcg
      121 cctccagcga cgtgcgctgc gcgcgatagg cggcgccgag ctggcgatcc agcccggggg
      181 acgcctggag catgcctgcc agcttcgcct tcccaacccg caccacctcg agcttcgcgc
      241 gtcgatcgag gcccaggtcg tcgagcagca ggtcgatgcc gcggagggtg agctgccatc
      301 gcgcgatctc gccggcgtcg ccctggaggt ggtcgaggat ctcggcgacg gcgtcgctgt
      361 cggcgtggaa catgtgctcc gcgatctcga tcccgtgggg gccgccgaag cgctcggtct
      421 cgcgctgata ggtgtcgagc gcgacgttcc agatcacgcc gtgctgcacg agcgcggccg
      481 ccctggcctg aagcgccggg aagacctcgc cgagcagccg cgcaggatct ccgtggaccc
      541 gcacccggag gcgcgcgcca tgatcggcat agcggaggaa gaaccagcgg tcggcggctc
      601 cgcggatccg cgcgtcggcg acccagggcc tgaccagctc cgtgatcacc cggtccgccg
      661 agcccagtcc ggtgtggaga tcggcctgca gccattccga gcccggcagg aagcgccgcg
      721 cgatgcgcgc cggcggggcg acgggctcgc tctcgaggtg cgtcaggttg tcgcgcagga
      781 acgggatcac cagctcgtgg acgaagcggc cgtccggtcc ggcggcgcac agctcctcga
      841 acgccgggaa cggctcgcgc aggaccacat cgcggcggcc cctggcggca tggccgagca
      901 cctcgacgca cagcgcgttg tcgagatcga gcggcaacca gcgctcggac tcgccgatct
      961 cgacgtgacg cgggatcccg agctccgcgc gaagctgctg gaccgcgcgg aaccgggcca
     1021 cgccgggggt cgcgagcccg tgcaacgcat ccgcgaacag ccgccagcgc gcccgggcga
     1081 ggatcacgcg gccaaactgc acccgcggca gaaccgccag ggcctcgagc ggcccccagc
     1141 ggaacgcgag gtcggtcgca gttccctggt gctgcagcgc gcacaggaac cggaccacgc
     1201 cgatccccgg ggagctcgcg ccgacggggg cggtcattct cgggatgacc tcgcagccca
     1261 ggcgcagcga acgcacgacg atccgctctg cgcgatcgat gcacaccacc agatcgctga
     1321 gtgggatctg gcgttcttcc ggcgcaccgg agcgggcgag gatcgggatc tcgtggccgc
     1381 gcagcaccgg ccggaccatc aggtcaccgg tctcgccctc cgggagaacc accagctctg
     1441 cgaacaccgc cccggggcgg accgcctcct cggcgcgcag cgacccggac agcctggcca
     1501 ggagcgggtc gtcgccgcgc cagcacgcac cgagcaggtt ggctcccgag ggccctgcgg
     1561 cgctctcgat cagcaccgtg aagtctccac cggacagcgc ctgccgggaa cgcgccgcga
     1621 tccgggccac cacgtggaac gcagccggaa gcggcagcgc gtcgtcgtga cccggccgca
     1681 gcagctcgtc gagcgacagg tcgaggcggt gctggccgtt gcgccgcgcc tgctcgacct
     1741 tgcgcagcaa cagggcgtcg cgcgcgg
//
LOCUS       DASVOR010002116         4846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_227345,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002116 DASVOR010000000
VERSION     DASVOR010002116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4846
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227345"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..1656
                     /locus_tag="VF516_39210"
     CDS             202..1656
                     /locus_tag="VF516_39210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113846.1"
                     /translation="MGGVFEEVVMKDSGGAWTWVFAGIALAAAVAVTVFGCAPPDARA
                     DAVPTRGETSYMSTIEPPFEKVFAELSREKPLVMQRALALLRDRYDLSDRSVRGVTMT
                     RGKPVQGGVRVKLPRGVTWQQLAAMSPEEIKRRGLWPAGFLPLPHPDQPQGGMVFPEF
                     LIREIRRQEDRDLTRFDVDFDIPDRFLPEFPPPIFLTTRPELGDVSQGELVTIENFYR
                     LFNGILNPKQIEGFRLLVTPFPQQQFNQTADRRSVKPSRGVACFDCHSNGHTNAAIHL
                     VQDARPQPFRRRVDTPTLRGVNQQRLFGSQRALKTVEDFTEFEQRGAYFDGDFPIAVK
                     KGINILDRGHQVHAMAELQELLDFPPAPKLDVYQHLYRSKATASELRGEQLFFSKARC
                     AECHPTPIFSDNFSHDLKTERFYEPTLVNGRYETAGGPTKTFPLRGIKDSPPYLHDGR
                     LLTLDDVIEFFNLVLETKLTEDEKRDLLAFLYCL"
     gene            complement(1703..1981)
                     /locus_tag="VF516_39215"
     CDS             complement(1703..1981)
                     /locus_tag="VF516_39215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113847.1"
                     /translation="MRRSFGLWLLGLGACYEVPGIDASQVITISPEKMSMPADGFSTV
                     DLDVTLDADAAVSQAITVTVSDGTVNFAGWPTRWSRPRAPPTARHRRP"
     gene            complement(1978..2250)
                     /locus_tag="VF516_39220"
     CDS             complement(1978..2250)
                     /locus_tag="VF516_39220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113848.1"
                     /translation="MASGEEFEVDLSAMALPLSDAAQDTIVFASSIAGCVVEPPSIDL
                     AIAAHDNRVTKHFRMRIRSGSDEITEITARARNSGSADSFVIRVIV"
     gene            complement(2429..3316)
                     /locus_tag="VF516_39225"
     CDS             complement(2429..3316)
                     /locus_tag="VF516_39225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113849.1"
                     /translation="MTRTKSLPARASGTSVFYDRLQDRQFDTQGQLLFPDGTPPDNPT
                     GINGGPPNPDAHPFWIPEFFGDVMTVNGKSWPFFEVQPRRYRFRVVNASNARFIQAQL
                     VHSANHQPTATPGPDIWQIGSDGGFLNAPTDLDAGASAPHLFLAPAERADIIIDFSGQ
                     SGKNFILINGQGAFAPFPSGDPPDPNTNGQIMEFRVNQTLQGTDTSFHPAHPQRALRA
                     SPIVNIKPASTGKTPDKKRQLILVEVEGAGGPLEVTTRSTLLTSGRCSTAPGRTPGPS
                     SRRRSDRRCLRTSTRPQRS"
     gene            complement(3998..>4846)
                     /locus_tag="VF516_39230"
     CDS             complement(3998..>4846)
                     /locus_tag="VF516_39230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEX8113850.1"
                     /translation="LMFLITGHVEPWPVAAGGSQAIARALASLLGAAGGRIETGIRVR
                     SLADLPPARVYLFDTSPAQLADIAGPVLPARYVRRLRRYRYGPGVFKLDWALDGAIPW
                     RDPRCLEASTVHVGGSLEQIAAAEAAVWRGEHPEQPFVMVCQQSQFDLTRAPPGKHTG
                     YAYCHVPAGSTVDVTDSIERQIERFAPGFRDRILARRATAPGDLERDNPNYVGGAITG
                     GVADVVQLFARPVARLDPYSTPHPRVLLCSASTPPGGGVHGMCGYHAARSAHRRLARH
                     PIAPLT"
BASE COUNT          745 a         1598 c         1740 g          763 t
ORIGIN      
        1 cgagcctacg acgtgcgtct gtcaatcacc cgcgcgcctc gtctcccacc cggggaccac
       61 cggagcgcgc gccgggctgg cgagcccgcc gtgcgccgtg ccccgtgcgc cgccgccgcg
      121 cgctccctcg cggcgccacg aaacgcgccg gtgtccacga gatccccggg cgagctccgc
      181 ggacccctgc ttgcactggc cgtgggcggc gttttcgagg aggtcgtgat gaaggactcg
      241 ggcggcgcat ggacgtgggt gtttgcgggg atcgcgcttg cggccgcggt ggcggtgacg
      301 gtgttcgggt gcgccccgcc ggacgcccgg gcggatgcgg tgccgacgcg gggcgagacc
      361 agctacatgt cgacgatcga gccaccgttc gagaaggtgt tcgccgaact gtcgcgggaa
      421 aagccgctcg tgatgcagcg cgcgctcgcg ctgctccgcg atcgctacga cctctccgat
      481 cgttcggtcc gcggcgtgac gatgacgcgc ggcaagccgg tccagggcgg cgtgcgcgtc
      541 aagctgccga ggggcgtcac ctggcagcaa ctcgcggcga tgtcgcccga ggagatcaag
      601 cggcgcgggc tgtggccggc cggcttcctg ccgctgccgc atcccgatca gccccagggc
      661 ggcatggtgt tcccggagtt cttgatccgg gagatccggc gccaggagga tcgggacctg
      721 acgcggttcg acgtcgactt cgacatcccc gaccgatttc tgcccgagtt ccccccgccg
      781 atcttcctga ccacgcggcc cgagctcggc gacgtgtccc agggcgagct ggtcaccatc
      841 gagaacttct accggctgtt caacggcatc ctcaacccca agcagatcga gggattccgc
      901 ctgctggtga cgccgttccc gcagcagcag ttcaaccaga ccgccgaccg gcgcagcgtg
      961 aagccgagcc gcggcgtggc gtgcttcgac tgccactcca acggccacac caacgccgcg
     1021 atccacctgg tccaggacgc ccggccccag ccgttccgcc ggcgggtcga tacgccgacc
     1081 ctgcgcggcg tcaaccagca gcgcctgttc ggatcgcagc gcgcgctcaa gaccgtcgag
     1141 gacttcaccg agttcgagca gcgcggggcg tacttcgacg gcgacttccc gatcgcggtg
     1201 aagaagggca tcaacatcct ggaccgcggc caccaggtcc atgcgatggc cgagctccag
     1261 gagttgctcg acttcccgcc cgcgcccaag ctcgacgtct accagcacct ctaccgcagc
     1321 aaggcgaccg cgtccgagct gcgcggcgag cagctgttct tcagcaaggc gcggtgcgcg
     1381 gagtgccatc cgacgccgat cttctcggac aacttctcgc acgatctgaa gaccgagcgg
     1441 ttctacgagc cgacgctggt caacggccgg tacgagaccg ccggcgggcc gaccaagacg
     1501 ttcccgctgc gcggcatcaa ggactcgccg ccctatctgc acgacggccg gctgctcacg
     1561 ctcgatgacg tgatcgagtt cttcaacctc gtgctcgaga ccaagctgac cgaggacgag
     1621 aagcgcgatc tgctcgcctt cctgtactgc ctgtgaccgc ggccgcggtc atgtggttga
     1681 tcctatgggt gcagggtcag cgtcacggac gacgatgtct tgccgtcggc ggcgcacgcg
     1741 ggcgtgacca tcgggtcggc caccccgcga agtttacggt gccgtcggag acggtcaccg
     1801 tgatcgcctg ggagactgcc gcgtccgcgt cgagcgtgac atcgagatcg accgtcgaga
     1861 aaccgtcggc cggcatgctc atcttctccg ggctgatcgt gatgacctgc gatgcgtcga
     1921 tgcccgggac ctcatagcaa gccccgagcc cgagtaacca gaggccgaac gaccgcctca
     1981 tacgatcact ctgatgacga acgagtcggc gctgccgctg tttcgggcac gggccgtgat
     2041 ctcggtgatc tcgtccgagc ccgagcgaat gcgcatgcgg aagtgcttgg tgacacgatt
     2101 gtcgtgggcg gcgatcgcga gatcgatcga tggcggctcg acgacacacc ccgcaatgga
     2161 gctcgcgaac acgatggtat cctgggcggc atcgctcagc ggcaacgcca tcgccgagag
     2221 gtcgacctcg aactcctcgc ccgacgccac ggtcttacgc tgacttgctc ggcgagcgcc
     2281 gccgccgagc agtgggagag cgcggtggcg tccgtcgcgt tcgcgagcgc ctcggcctga
     2341 tcgtgcaagc tcttgccctc cggcagcagc gccagcgggc tgccgtcgat cagcaccgag
     2401 ctcggcgtgg ctccgctacg gaacggggct agcttcgttg cggccgcgtc gatgtccgga
     2461 ggcagcggcg atccgaacgc cttctggagc tcggtcctgg ggtccgcccc ggtgccgtgg
     2521 agcaccttcc agaggtcagc aacgtgctcc tggtcgtgac ctcgagcggg ccgccggcgc
     2581 cctcgacctc gaccaggatc agctggcgct tcttgtcggg cgtcttgccg gtgctggccg
     2641 gcttgatgtt gacgatcggc gaggcgcgca gcgcgcgctg cgggtgggcc gggtggaagc
     2701 tggtgtcggt gccctgcagc gtctggttga cgcggaactc catgatctgg ccgttggtgt
     2761 tcggatcggg cggatcgccg ctcgggaacg gcgcgaacgc gccctggccg ttgatcagga
     2821 tgaagttctt gccgctctgg cccgagaagt cgatgatgat gtcggcgcgt tccgcgggcg
     2881 cgaggaacag gtgcggcgcg ctggcgccgg cgtcgaggtc ggtcggcgcg ttgaggaagc
     2941 cgccgtccga gccgatctgc cagatgtcgg ggccgggcgt ggcggtcggc tgatggttgg
     3001 ccgagtggac cagctgggcc tggatgaacc gggcgttcga cgcgttgacg acgcggaacc
     3061 ggtagcggcg gggctggacc tcgaagaacg gccacgactt gccgttgacc gtcatgacgt
     3121 cgccgaagaa ctcggggatc cagaacgggt gagcgtcggg gttgggcgga ccgccgttga
     3181 tgccggtcgg gttgtcgggc ggcgtgccgt cggggaacag cagctggccc tgggtgtcga
     3241 actggcgatc ttggagccga tcgtagaata ctgatgtgcc ggacgcgcga gccgggagtg
     3301 attttgtgcg agtcacgccg ccccggatcg ctcgccgtcc cgccgcgttg ttgagaacgg
     3361 gtcaatgtgg ccagtggttt gtcgcagcca ttgaattgaa aatcgttttc cgtggaacca
     3421 ccgcgatccg ttcaccaatc acctcgccgt gaaaccgccc gacgccggac ggatgcgcct
     3481 cggggcgtcc agcgcgcgcc ggtcgcgtgc tcgtcgaggc caccgcagcg tgcggcgcgg
     3541 cgcggcgcgg cgcggcatcc ggcatctcgg atcgcacgca tggtcaggtc gcgcacggtc
     3601 gccggaggct ccgccagaaa actgagtggt cgcaagaaca gcgttgctgg gatcagggcg
     3661 aggcaactcg cctcaacagc cgtcgaacct tcgttcgccg gctcaagatg tctagaaaat
     3721 tgagcttgcg ctgagtccga ggccgttgag gttgaccttg aggttgtcgc agggcgttga
     3781 cctggagggc gacgtagtcg ttgagcccgt cgtggacctt ggacgtgaac cccgggtcat
     3841 ttgcgaggag tcgtcctgga gaatgacgag gtatgggcaa ggtcgaggtc gacggtgggg
     3901 gcgacgtcct acgttgccgt caaggcccac gatggagtaa cgacgaggtc aacctcgagg
     3961 tgggggatcc agatcgaacg ctttgatcga cacatcttca ggtgagcggg gcgatcgggt
     4021 ggcgcgcgag ccggcggtgc gccgaacgcg cggcgtgata accgcacatg ccgtggacac
     4081 cgcctcccgg cggcgtcgac gccgagcaca gcagaacgcg cggatggggt gtcgagtacg
     4141 ggtcgagccg cgcgaccggc cgggcgaaca gctggacgac atcggcgacg ccgccggtga
     4201 tcgcaccgcc gacgtagttg ggattgtcgc gctcgaggtc gcccggcgcg gtggcgcggc
     4261 gggcgaggat gcgatcgcga aaccccggcg cgaaccgctc gatctggcgc tcgatcgagt
     4321 cggtgacgtc gacggtcgag ccggccggta cgtggcagta ggcatagccg gtgtgcttgc
     4381 cgggcggcgc gcgcgtgaga tcgaactggc tctgctggca gaccatcacg aacggctgct
     4441 cgggatgctc gccgcgccac accgcggcct ccgcggcggc gatctgctcc aggctgccgc
     4501 cgacgtgaac ggtcgacgcc tccaggcagc gcggatcgcg ccacgggatc gcgccgtcga
     4561 gcgcccagtc gagcttgaac acgcccggtc cgtagcgata ccggcggagc cgccggacgt
     4621 agcgggcggg cagcaccggc ccggcgatgt ccgcgagctg ggccggactg gtgtcgaaca
     4681 ggtagacccg ggcgggcggc agatcggcga gcgagcggac ccggatcccg gtctcgatcc
     4741 ggccgcccgc ggcgccgagc aggctcgcca gcgcgcgcgc gatcgcctgc gagccgccgg
     4801 cggcgaccgg ccagggctcg acgtggcccg tgatcaggaa catcag
//
LOCUS       DASVOR010002117         2264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_343489,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002117 DASVOR010000000
VERSION     DASVOR010002117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343489"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /gene="gspN"
                     /locus_tag="VF516_39235"
     CDS             complement(<1..1152)
                     /gene="gspN"
                     /locus_tag="VF516_39235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspN"
                     /protein_id="HEX8113851.1"
                     /translation="MATLFASLGPRARKLLRYASLVVLALVSFVFALQLTFPYDRVKD
                     AAILALSEKGYDAQIGDVERGILPARVYFKAVTLRTRPTKAGDVATTFYIEQLEVDLG
                     LLPLLHGTVAVKLDAKIGQGHLKGNLALSKDGTSVHVTGDDLPSANLPVREALGLPMS
                     GKVRLAFNLELPNDRSKTEPKTGRAGPNWPKAEGNAEFACPSGCTVGDGKSKLKLSTN
                     NARQQEFLDQGGGGIDFGKVNVDSLLASVELKNGKLELTRFELKSPDGEAKIVFSLAL
                     NQDLMASAVTGCLRFHGSEALRKREPKTDAALSTTGAPLGPDNLFHIKLDGQLRTMRR
                     IGVLCGPAAGGGNETPGSPPPRPNLTISPEPARPPGVGSVGFPTPSPPPT"
     gene            complement(1154..1744)
                     /gene="gspM"
                     /locus_tag="VF516_39240"
     CDS             complement(1154..1744)
                     /gene="gspM"
                     /locus_tag="VF516_39240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HEX8113852.1"
                     /translation="MSAVSERARDFWDRISPRERGLVMIAAVVAPLTLVIWLGLAIGD
                     GLAGMQARNDKMRKALAVLADLKARGPRQPVDDVVSGMPVEPLSLETYLSNAATTAGF
                     VLKGTTPRNPVTRNGFVTASVSLNASDLTVEQLKKFLQEIETKSKYVAITRMDITRKE
                     YKGKDKLDVAMEVSTYAKEPAKKPDAGSGAGSEKGS"
     gene            complement(1747..>2264)
                     /locus_tag="VF516_39245"
     CDS             complement(1747..>2264)
                     /locus_tag="VF516_39245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113853.1"
                     /translation="VDFSFLRAKAVPLGAAVVAIAAVAAGSAYADLYRLRKAEKLLTT
                     RLAEESAEPFGSAKTAEQVLDANGPAGTAAASPMPRLSAYDLLLDISAHIPGTDKITL
                     DIEKMTIDDQKIDINGTTKSAEEIDLLISEIKKIECFKNNLTRGPTDTLPSGAKRFKL
                     TITAQCLMGAS"
BASE COUNT          292 a          748 c          821 g          403 t
ORIGIN      
        1 cgtgggcggc ggcgacggcg tcgggaaccc gaccgagccg acgcccggcg gcctggccgg
       61 ctccggagag atcgtgaggt tgggccgcgg cggcgggctg cccggggtct cgttgccgcc
      121 gcccgcggcc ggtccgcaca gcacgccgat ccggcgcatc gtgcggagct ggccgtcgag
      181 cttgatgtgg aacaggttgt cggggccgag cggcgccccg gtggtcgaca gcgcggcgtc
      241 ggtcttgggc tcgcgcttgc ggagcgcctc gctgccgtgg aatcgcaggc agccggtgac
      301 ggccgacgcc atgaggtcct ggttgagcgc caggctgaac acgatcttcg cctcgccgtc
      361 gggcgacttg agctcgaacc tggtgagctc gagcttgccg ttcttgagct cgacgctcgc
      421 gagcagcgag tcgacgttga ccttgccgaa gtcgatgccg ccgccgccct ggtcgaggaa
      481 ctcctgctgg cgggcgttgt tggtggacag cttgagcttg gacttgccgt cgccgacggt
      541 gcagcccgac gggcacgcga actcggcgtt gccctcggcc ttgggccagt tgggccccgc
      601 tcttccggtc ttgggctcgg tcttgctccg gtcgttgggc agctcgaggt tgaacgcgag
      661 ccggaccttg ccgctcatcg gcaggccgag cgcctcgcgc accggcaggt tcgccgacgg
      721 caggtcgtcg ccggtcacgt ggaccgacgt cccgtccttc gacagcgcga ggttgccctt
      781 gaggtgaccc tggccgatct tggcgtcgag cttgaccgcg accgtgccgt gcagcagcgg
      841 cagcagaccg aggtcgacct cgagctgctc gatgtagaac gtggtcgcga cgtctccggc
      901 cttggtcggc cgggtccgca gcgtgaccgc cttgaagtac acccgggcgg ggaggatgcc
      961 gcgctcgacg tcgccgatct gggcgtcgta gcccttctcg gacagggcga ggatcgccgc
     1021 gtccttgacc cggtcgtagg ggaacgtcag ctggagcgcg aacacgaagc tgaccagcgc
     1081 gagcacgacc agggacgcgt agcggagcag cttgcgcgcg cgcgggccga ggctggcgaa
     1141 cagcgttgcc atgtcagctg cctttctcgg agccggcgcc cgagcccgcg tcgggcttct
     1201 tggccggttc cttggcatag gtcgagacct ccatcgccac gtcgagcttg tctttgccct
     1261 tgtactcctt gcgcgtgatg tccatccggg tgatcgcgac gtacttggac ttggtctcga
     1321 tctcctgcag gaacttcttg agctgctcga ccgtcaggtc gctggcgttg agcgacaccg
     1381 acgcggtgac gaagccgttg cgggtgaccg ggttgcgcgg cgtcgtgccc ttgagcacga
     1441 agcccgcggt ggtcgccgcg ttgctgaggt aggtctcgag gctgagcggc tcgaccggca
     1501 tgccggacac cacgtcgtcg accggctggc gcgggccgcg cgccttgaga tcggccagca
     1561 cggcgagcgc cttgcgcatc ttgtcgttgc gggcctgcat gccggccagg ccgtcgccga
     1621 tcgcgagccc gagccagatc acgagcgtca gcggcgcgac caccgcggcg atcatgacca
     1681 gcccgcgctc gcgcggcgag atccggtccc agaagtcacg tgcgcgttcc gatacggcag
     1741 acatggctag ctcgccccca tcaggcattg cgcggtgatc gtgagcttga accgcttggc
     1801 gccgctgggc agcgtgtcgg tcggtccgcg cgtcaggttg ttcttgaagc actcgatctt
     1861 cttgatctcg ctgatcagca gatcgatctc ctcggccgac ttggtcgtgc cgttgatgtc
     1921 gatcttctgg tcgtcgatcg tcatcttctc gatgtcgagc gtgatcttgt ccgtgcccgg
     1981 gatgtgggcg ctgatgtcga gcaggaggtc gtaggcgctg agcctgggca tcggcgaggc
     2041 cgccgcggtg cccgccgggc cgttggcatc gagcacctgc tcggcggtct tggccgagcc
     2101 gaacggctcg gccgactcct cggcgagccg ggtggtcagc agcttctcgg ccttgcgcag
     2161 ccggtacaga tcggcgtacg cgctgccggc ggcgaccgcg gcgatcgcga ccaccgcggc
     2221 gccgagcggc accgccttgg cgcgcaggaa cgagaagtcg acct
//
LOCUS       DASVOR010002118         8138 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_52434,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002118 DASVOR010000000
VERSION     DASVOR010002118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8138
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52434"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="VF516_39250"
     CDS             <1..479
                     /locus_tag="VF516_39250"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8113854.1"
                     /translation="RQGRAASEPGASASPDPGPRGRMPLPDLGGLEVLVVDDERDGRD
                     VISEILERAHARVTVAASSAEAMDVLARLRFAVLVSDIGMPDEDGFTLMRRVRALPEH
                     RGGRIPAVALTAYARAIDRQQAMMAGYDIHLGKPVDPGELVDVVASLAARGDAAPE"
     gene            complement(480..1139)
                     /locus_tag="VF516_39255"
     CDS             complement(480..1139)
                     /locus_tag="VF516_39255"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEX8113855.1"
                     /translation="MPRWIAVMLVLGASVAARADMYSWTDSEGVIHFTNMKPSGGKWK
                     KVVLDQPDFGSKASARRGSCERCDKVAATDTSPERFHRYDPFILEASQLYRIPVPLIR
                     AVIKVESDYDTRVVSSMDCKGLMQVHPQVEIDMGAQGDIFDPRTNIMTGTRLLRWLAN
                     RVDGDLVLTIAGYHAGLGSLARYGYTVPPYAYTRQYLKMVLERYYQYKAEEQRAQAAG
                     R"
     gene            1197..2786
                     /gene="nadB"
                     /locus_tag="VF516_39260"
     CDS             1197..2786
                     /gene="nadB"
                     /locus_tag="VF516_39260"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEX8113856.1"
                     /translation="MAESLETDYLVIGSGIAGLNFALLAAEHGRVVVLTKKQPDDTNT
                     NWAQGGVAAALSSDDSFEQHLQDTLTAGDGLCDRDVVELCVAQGPAMVQRLLDIGVRL
                     ARTPDGQLDLGREGAHTHHRVVHWQDITGREIQRALLEAVRRDARITMLHDHIAVDLL
                     SMAKYGGDPACFGAYVLDRTTGEVKTLLARATVLATGGTGKVYVYTSNPDVATGDGIA
                     MAYRIGAACCDLEFVQFHPTVLYHPHARNFLLTEAMRGEGGVLRLATGETFMEHYHPM
                     KSLASRDIVARAIDNELKKSGADSVFLDMTRLDPAFVRGRFPNIHERCLGLGIDITKQ
                     PIPVVPAAHYMCGGVKTDHDGRTTIDGLYAIGECAFTGLHGANRLASNSLLEGMVYST
                     RTAAAVRDRPRLRPSVVAPWSSGNATDSNDAIVVTLNWDEIRRFMWSYLGIVRSDKRI
                     ERARRRIEILRDEIREYYIDFKITSDLIELRNLALVAHLIIESARRRKESRGLHATLD
                     YPNKLPEARHSEIQLTDGPRG"
     gene            2892..4310
                     /locus_tag="VF516_39265"
     CDS             2892..4310
                     /locus_tag="VF516_39265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113857.1"
                     /translation="MSLCAACGDDGPDRPPLPQVTTFGGPIMAHPQLVPIFYNDDTDT
                     IAVMSFSRWVVTSDWLADVGAEYGVGPGSVLGMVQLPMPAPASLTDAQVVDLMFQGVT
                     TGSLPRPADGNLGDVLYVLYLPPRTTETLGNLSSCADFGGYHSSARRNGVELSYAVIV
                     TCPNNGPQLTNVEFREYVTSHELIEAATDPVPGNHPAYRLNDSTSPWISTGGEVGDLC
                     ELSGRRVFRDSGYVVQRSWSNLAAQSGDPCVPIPAKTTYFNVRVDGQYPPRIPVGGHQ
                     TLQLTGWSTGTEPGWMLEADAAKPGQVTLTLGATQINAGRTTTLDIAFPATARPGTVA
                     AFYVFSVLPDRTYETLPMRAIVGDPCASFSGCEACTAQPGCGFCATTGRCENRGASGS
                     ADSSCPAASFARSLGDCPGFCASHAGSCVDCASQPGCGWCDAGAASQCVAIDHKTDLP
                     ANNACSYESWDLVPDYCPLPGP"
     gene            4437..5093
                     /locus_tag="VF516_39270"
     CDS             4437..5093
                     /locus_tag="VF516_39270"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEX8113858.1"
                     /translation="MKRRFHDSPLLPGTALGPAECLARAQPLEGTDGQAYVERRGIAV
                     AIATAAGVRFCPDWAGRPAVVIALRDRDGEIRSVHGRYLHNVRGEDKMLTIGAGGGAI
                     CVLGGWRSDPLIVVEGLFDALSLAVCGHAAVATIGRDVPWLASIAAGRTVHIASDRTR
                     PGEQDAARLAAALIGARVHRLPPPPRCKDWNTALVKRGPSAVAKWLRDNVGRSASVGP
                     "
     gene            5090..5650
                     /locus_tag="VF516_39275"
     CDS             5090..5650
                     /locus_tag="VF516_39275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113859.1"
                     /translation="MTEIYVSTDVEADGPIPGPHSMLSFASAAFLPDKTCVSTFSANL
                     ALLPGAAGHPDTMAFWRSHPDAWEAARRDPRDPAQAMTAYVRWVDGLEGKPVFVGYPA
                     AFDFMFVYWYLIKFVGRSPFSHSALDIKTMAMVLLGKGYRESTKKHMPRRWFDDVPHT
                     HVALDDAIEQGRLFCNMLAEIRKKSG"
     gene            5647..6471
                     /locus_tag="VF516_39280"
     CDS             5647..6471
                     /locus_tag="VF516_39280"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEX8113860.1"
                     /translation="MRRGFPQSVCFRVTRACNARCGFCLAPWDGAHPSAEVLLHRIDW
                     LVDRGVRTVHFCGGEPTIHPGLGSLIARTRERGAVPRLTTNGIAMPEPLLEALRRHRV
                     DVKVSLHGGRAWHDRMVGRPAYEAATATLRRLLSAGVAASVQTTLVAGGGDVLAEMIA
                     FCLDAGVKRLSLLPFIPRGDGAAREAEFGLSDGERRALRQAVAAQRRRLGGRLDLRWL
                     DFRTDRAPVVEADGRVLLEGPREASDTLLAVIPAPRAVAPGDGEDRPARLRVLSPP"
     gene            6628..8061
                     /locus_tag="VF516_39285"
     CDS             6628..8061
                     /locus_tag="VF516_39285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113861.1"
                     /translation="MLVPFVMGCPDRSIDKIPPTQSGVVVKKIAVSADIDLLFVIDNS
                     GSTIDKQTVFSQNFPRFVQALDAFPMGRPNLHIGVVDTTVDIETPGYANGNTGCPSPD
                     PRDNGLLQNIPKIPGCTPPNGQFITDTKNPAGSRTVNYTGTLEQALSCIALIGAEGCG
                     FEAPLEAMKRALDGSRPENAGFIRNGAYLGVIILTDEDDASVKDHAVFTLPDATVGGK
                     SDFRVQPLLAYQCTTPISSSTGGTYHHCTPRTGSYLQDPHDYSQFLTTVKDPSQIVVA
                     AIAAPPPGLQTNDDPPRTASSPDITTGSLTLNGTTQALALLPSCSATINGNPAIGRPG
                     IRLASFIGDFGDRGRFYTVCQSDYSAALADIGKTLFNAISPCLEGPIDPTDADPSNPG
                     TQLQCTVSDVRVDDQIGTTSTVIPACKMQDATTPAATGARPCWWVDQNPVACPAPDTG
                     FELELVRSQPPAPGTNVEVECALLPAN"
BASE COUNT         1221 a         2973 c         2761 g         1183 t
ORIGIN      
        1 cccggcaggg ccgcgccgcc agcgagcccg gcgcgagcgc gtcgccggat ccggggccgc
       61 gcggccgcat gcccctgccc gatctcggcg gcctggaggt cctggtcgtc gacgacgagc
      121 gggacgggcg cgacgtgatc agcgagatcc tcgagcgcgc ccatgcccgg gtcacggtcg
      181 cggccagctc cgccgaggcg atggacgtgc tcgcccggct ccggttcgcc gtcctggtgt
      241 cggacatcgg catgcccgac gaggacggct tcaccctgat gcgccgggtc cgcgcgctgc
      301 ccgagcatcg cggcggccgg atccccgccg tcgcgctgac cgcgtacgcg cgcgccatcg
      361 accgccagca ggcgatgatg gccggctacg acatccacct cggcaagccg gtcgatccgg
      421 gcgagctggt cgacgtggtg gcgagcctgg ccgcgcgcgg cgatgccgcc ccggagtagt
      481 cagcgccccg ccgcctgggc gcgctgctcc tcggccttgt actggtagta gcgctcgagc
      541 accatcttca ggtactgccg cgtgtacgcg tacggcggca cggtgtagcc gtacctggcc
      601 agcgagccga ggccggcgtg atagccggcg atcgtcagca cgaggtcgcc gtcgacccgg
      661 ttggcgagcc agcgcaagag ccgcgtcccg gtcatgatgt tggtgcgcgg atcgaagatg
      721 tcgccctgcg cgcccatgtc gatctcgacc tgcgggtgga cctgcatcag gcccttgcag
      781 tccatcgacg acacgacgcg ggtgtcgtag tcgctctcga ccttgatcac cgcgcggatc
      841 agcggcaccg ggatgcggta gagctgcgac gcctcgagga tgaacgggtc gtagcggtga
      901 aaccgctcgg gcgacgtgtc ggtcgccgcg accttgtcgc agcgctcgca gctgccccgc
      961 cgcgcgctcg ccttggaccc gaagtcgggc tggtcgagca cgaccttctt ccatttccca
     1021 ccagacggct tcatgttggt gaagtggatg acgccttcgc tgtcggtcca tgaatacatg
     1081 tcagcgcggg ccgcaacgct ggcccccaga acgagcatca cagcgatcca ccgcggcacg
     1141 acgaaatcgt agcccggcta cctacggagc gcaaaagcag tatggtcgca cctcacatgg
     1201 ccgagtccct ggagaccgac tacctggtga tcggatccgg catcgccggc ctcaacttcg
     1261 ccctcctggc cgccgagcac ggccgcgtcg tggtgctcac caagaagcag cccgacgaca
     1321 ccaacaccaa ctgggcgcag ggcggggtcg ccgcggcgct gtcgtccgac gacagcttcg
     1381 agcagcacct ccaggacacc ctgaccgcgg gcgacgggct gtgcgaccgc gacgtcgtcg
     1441 agctgtgcgt cgcccagggc ccggcgatgg tccagcgcct gctcgacatc ggcgtgcggc
     1501 tggcgcggac ccccgacggc cagctcgacc tcggccgcga gggcgcgcac acccaccacc
     1561 gggtcgtgca ctggcaggac atcaccggcc gcgagatcca gcgcgcgctg ctcgaggcgg
     1621 tgcgccgcga cgcgcggatc acgatgctcc acgatcacat cgcggtcgac ctgctgtcga
     1681 tggccaagta cggcggcgat ccggcgtgct tcggcgcgta cgtgctcgac cgcaccaccg
     1741 gcgaggtcaa gaccctgctc gcgcgcgcga ccgtgctcgc gaccggcggc accggcaagg
     1801 tctacgtcta cacctcgaac cccgacgtcg cgaccggcga cggcatcgcg atggcctacc
     1861 ggatcggcgc ggcgtgctgc gacctcgagt tcgtccagtt ccaccccacc gtgctctacc
     1921 acccgcacgc gcgcaacttc ctgctcaccg aggcgatgcg cggcgagggc ggcgtgctcc
     1981 ggctcgcgac cggcgagacc ttcatggagc actaccaccc gatgaagtcg ctcgcatcgc
     2041 gcgacatcgt cgcccgggcg atcgacaacg agctcaagaa gtcgggcgcc gactcggtgt
     2101 tcctcgacat gacccgcctc gacccggcgt tcgtgcgcgg ccggttcccc aacatccacg
     2161 agcgctgcct cggcctgggc atcgacatca ccaaacagcc gatcccggtc gtccccgcgg
     2221 cgcactacat gtgcggcggg gtcaagaccg atcacgacgg ccggaccaca attgacgggc
     2281 tgtacgcgat cggcgagtgc gcgttcaccg gcctgcacgg cgccaaccgg ctggcctcga
     2341 actcgctgct cgagggcatg gtgtactcga cgcgcaccgc cgccgcggtc cgcgatcggc
     2401 cgcggctccg gccgtcggtg gtcgcgccgt ggtcgtccgg caacgccacc gactccaacg
     2461 acgcgatcgt cgtcaccctc aactgggacg agatccgccg gttcatgtgg tcgtacctgg
     2521 gcatcgtgcg ctccgacaag cggatcgagc gcgcgcgccg gcggatcgag atcctgcgcg
     2581 acgagatccg ggagtactac atcgacttca agatcacgag tgacctgatc gagctgcgca
     2641 acctcgcgct ggtcgcccac ctgatcatcg agagcgcccg gcggcgcaag gagtcgcgcg
     2701 gcctgcacgc cacgctcgac tacccgaaca agctgcccga ggcccgccac tccgagatcc
     2761 agctgaccga cggcccgcgc ggctagcaag ccactcggca gccagcggtt gcgcgcccca
     2821 ccgccgcgtg cgatcgtcgg ccccgcgact gacaaggggg atccatgcgg gcctgggcaa
     2881 tcatggtgtg tgtgagcttg tgtgcggcgt gcggggacga tggaccggat cggccaccgc
     2941 tgccgcaggt caccacgttc ggcgggccga tcatggcgca cccgcagctc gtgccgatct
     3001 tctacaacga cgatacggac accatcgcgg tgatgagctt cagccgctgg gtcgtcacct
     3061 ccgactggct tgccgacgtc ggcgccgagt acggcgtcgg cccgggctcg gtgctcggca
     3121 tggtgcagct gccgatgccc gcgcccgcct cgctcaccga cgcccaggtc gtcgacctga
     3181 tgttccaggg cgtcaccacc ggcagcttgc ccaggcccgc cgacggcaac ctcggcgacg
     3241 tgctctacgt gctgtatctc ccgccccgga cgaccgagac cctcggcaac ctgtccagct
     3301 gcgccgattt tggtggctac cacagctcgg cgcgccgcaa cggcgtggag ctgtcgtacg
     3361 ccgtcatcgt gacatgcccg aacaacggcc cgcagctgac caacgtcgag ttccgggagt
     3421 acgtcacgtc gcacgagctc atcgaggccg cgaccgatcc ggtgccgggg aaccaccccg
     3481 cgtaccggct gaacgactcg acaagcccct ggatctcgac cgggggcgag gtcggggacc
     3541 tgtgcgagct ctcgggccgc cgggtgttcc gggacagcgg ctacgtcgtg cagcgcagct
     3601 ggtcgaacct cgccgcccag tcgggagatc cgtgcgtgcc catcccggcc aagaccacgt
     3661 acttcaacgt ccgcgtcgac ggccagtacc ccccgcgcat cccggtcggc ggccaccaga
     3721 ctctccagct caccggctgg tcgaccggca cggagcccgg ctggatgctc gaggccgacg
     3781 ccgccaagcc cggtcaggtc acgctgacgc tcggcgcgac ccagatcaat gccggcagga
     3841 ccacgacgct cgatatcgcg ttccccgcca cggcacggcc cggcacggtc gcggcgttct
     3901 acgtgttctc cgtgctgccg gaccgtacct acgagacctt gccgatgcgc gcgatcgtcg
     3961 gcgatccgtg cgcgagcttc tccggttgcg aggcatgcac ggcccagccc ggatgcggct
     4021 tctgcgccac gaccggccgc tgcgagaacc gcggcgcctc gggatccgcc gacagctcgt
     4081 gtccggccgc gtcgttcgca cgatccctcg gcgactgccc cggattctgc gcttctcatg
     4141 ccgggagctg cgtcgactgc gcctcgcaac cgggatgcgg ctggtgcgac gccggcgcgg
     4201 cttcgcaatg cgtggcgatc gatcacaaga cggacttgcc ggccaacaac gcgtgcagct
     4261 acgagagctg ggacctcgtc ccggactact gcccgctccc cgggccgtga gcgcgtcgcg
     4321 cgcccgccgc aggtcttcga cgacgagcgg atccaggttc ggccggcgct gcgcgcgagc
     4381 ggatggtagc tgaccacgca cggccgcccg cccaccctac gccgccgagc tccgcgatga
     4441 agcgtcgatt ccacgattcc ccgctgttgc cgggtaccgc gctcgggccg gcggagtgcc
     4501 tggcccgcgc gcagccgctc gaggggaccg atggccaagc ctacgtcgag cggcgcggca
     4561 ttgccgtggc catcgcgacg gcggccggcg tgcggttctg tccggactgg gccggacggc
     4621 cggcggtggt gatcgcgctg cgcgatcgcg acggcgagat ccggtcggtg cacggccgct
     4681 acctgcacaa cgtccgcggc gaggacaaga tgctcaccat cggtgccggc ggcggcgcga
     4741 tctgcgtcct gggtggctgg cgcagcgatc cgttgatcgt cgtggagggc ctgttcgatg
     4801 cgctgtcgct ggcggtgtgc ggccacgccg cggtggccac gatcgggcgc gacgtgccct
     4861 ggctggcctc gatcgcggcc gggcgcaccg tccacatcgc ctccgatcgc acccggcccg
     4921 gcgagcaaga cgccgctcga ctggccgcgg cgctgatcgg cgcgcgcgtg catcgtttgc
     4981 cccctccgcc gcgctgcaag gactggaaca ccgcgctggt gaagcgcggg ccgtccgcgg
     5041 tcgcgaaatg gctgcgcgac aacgtcgggc gatctgctag cgtgggcccg tgaccgagat
     5101 ctacgtcagc accgacgtcg aggccgacgg gccgatcccc ggcccgcact cgatgctcag
     5161 cttcgcgtcg gccgcgttcc tgcccgacaa gacgtgcgtg tcgacgttct cggccaacct
     5221 ggcactgctg ccgggcgccg ccggccatcc cgacacgatg gcgttctggc ggtcacatcc
     5281 ggacgcctgg gaggcggcgc ggcgcgatcc acgcgatccg gcacaagcga tgaccgccta
     5341 cgtgcgctgg gtcgatggac tcgaaggcaa gcccgtgttc gtcggttatc ccgccgcgtt
     5401 cgatttcatg ttcgtgtact ggtacctgat caagttcgtc gggcggagcc cgttcagcca
     5461 ctcggcgctc gacatcaaga cgatggcgat ggtgctgctc ggcaaggggt atcgcgagag
     5521 caccaagaag cacatgccgc ggcgctggtt cgacgacgtg ccccacaccc atgtcgcgct
     5581 cgacgacgcg atcgagcaag gccggctgtt ctgcaacatg ctcgccgaga tccggaagaa
     5641 gagcggatga ggcgcgggtt tccgcagtcg gtgtgcttcc gcgtcacccg cgcgtgcaac
     5701 gcgcgctgcg ggttctgtct ggcgccctgg gacggcgcgc atccctccgc cgaggtactg
     5761 ctgcaccgca tcgactggct ggtggaccgc ggcgtccgca cggtgcactt ctgcggcggc
     5821 gagccgacca tccatccggg cctggggtcg ctgatcgcgc gcacgcgcga acgcggcgcc
     5881 gtcccgcggc tgaccaccaa cggcatcgcg atgccggagc cgctgctcga ggcgctgcgc
     5941 cgccatcgcg tcgacgtcaa ggtgagcctg cacggcgggc gcgcctggca tgaccggatg
     6001 gtggggcggc cggcgtacga ggcggccacc gccaccctgc ggcggctgct gtccgcggga
     6061 gtggcagcgt cggtgcagac cacgctggtc gcgggcggcg gcgacgtgct cgccgagatg
     6121 atcgcgttct gtctcgacgc cggcgtgaag cggctgagcc tcttgccgtt catcccgcgc
     6181 ggcgatggcg cggcccgcga ggccgagttc ggcctgtccg acggcgagcg ccgcgccctg
     6241 cgccaggcgg tggccgcgca acggcggcgg ctcggcggcc gcctcgatct gcgctggctc
     6301 gacttccgga ccgaccgggc cccggtggtc gaggccgacg ggcgcgtgct gctcgaagga
     6361 ccgcgcgagg cgagcgacac gctgcttgcc gtcatccccg cgccgcgagc ggtcgctccc
     6421 ggcgatggag aggatcgccc ggcccggctc cgcgtgctgt ctcctccgtg actcgccggc
     6481 gcgcagggtg gtcggcggcg tgagccgcgg tcgtgcccga gtctgccacc tgaccgggtg
     6541 cgctcgggat cgatcgccgt gacggatcgt ggggggtcgc gacacgaacc cgcagcagga
     6601 gacgccatgc gcgcttggcc acttggcttg ctggttccgt tcgtgatggg gtgccccgac
     6661 cgatcgatcg acaagatccc gccgacccag agcggcgtgg tcgtcaagaa gatcgcggtc
     6721 agcgccgaca tcgacctcct gttcgtgatc gacaactccg ggtcgacgat cgacaagcag
     6781 acggtgttct cgcagaactt cccgaggttc gtgcaggcgc tcgatgcgtt ccccatgggc
     6841 cgccccaacc tgcacatcgg ggtcgtcgac acgaccgtcg acatcgagac cccgggctac
     6901 gcgaacggca acacgggatg tcccagtccg gatccacgcg acaatgggct gttgcagaac
     6961 atcccgaaga tcccgggctg cacgccaccg aatggacagt tcatcaccga caccaagaat
     7021 ccggccggct cgcgaaccgt gaactacacc ggcacgctcg agcaagccct gtcgtgcatc
     7081 gccctgatcg gcgcggaggg ctgtggcttc gaggccccgc tcgaggccat gaagcgggcg
     7141 ctcgatggct cgcggcccga gaacgcgggc ttcattcgca acggggcata cctcggcgtc
     7201 atcatcctca ccgacgagga tgacgcctcg gtcaaggacc acgcggtgtt cacgctcccc
     7261 gacgcgacgg tcggtgggaa aagcgacttc cgcgtccagc cgctgctcgc ctatcagtgc
     7321 acgacaccga tctcgagctc gacgggcggg acctaccatc attgcacacc gcggaccggc
     7381 tcgtacctgc aggatccgca cgattactcg cagtttctga cgaccgtcaa ggatcccagt
     7441 cagatcgtcg tcgcggcgat cgcggcgcct ccgcccggtc tgcaaaccaa cgacgatcca
     7501 cctcggaccg cgagctctcc ggacatcacc accggctcgc tcacgctcaa cggcaccacg
     7561 caagcgctgg cgctgctgcc atcgtgctcg gccacgatca atggcaaccc ggcgatcggg
     7621 cggcccggca tccggctcgc gagcttcatc ggcgacttcg gcgaccgcgg acggttctac
     7681 accgtctgcc agagcgacta ctcggccgcg ctggccgaca tcggcaagac gctgttcaac
     7741 gcgatcagcc cgtgcctgga gggcccgatc gaccccaccg acgccgaccc atcgaacccc
     7801 ggcacccagc tgcaatgcac ggtcagcgac gtccgggtcg acgaccagat cggcacgacc
     7861 tccacggtga tccccgcctg caagatgcag gacgccacca cgcccgcggc gaccggggcg
     7921 cggccgtgct ggtgggtcga ccagaatccg gtggcgtgcc cggcaccgga caccggcttc
     7981 gagctcgagc tcgtgcgctc gcagccaccc gcgcccggca cgaacgtcga ggtcgaatgc
     8041 gccctgctgc ccgcgaactg agcggcgaga gcagcgctcg gggccggcgg cggacgcgac
     8101 gccggcgcac ggcgggcgag ccgcgctcac cggaagtg
//
LOCUS       DASVOR010002119         3679 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401857,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002119 DASVOR010000000
VERSION     DASVOR010002119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401857"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            630..1790
                     /locus_tag="VF516_39290"
     CDS             630..1790
                     /locus_tag="VF516_39290"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HEX8113862.1"
                     /translation="MEPWRSEWSEIHRRLREYARQRASLDAAEAFDLVRAEQLKLHFL
                     CGFATMYEYMERVLGYGPHAARERLRVARSLAKLPQTTAALARGALTFSAARELTRVA
                     TAETEGRWLTAAEGMTAGQIERLVAGHQPGDQPEDPTHPDLRSRVVRLELPPEVYALW
                     RQAHAIVAAERGAEISDADFVESLCRAVIAPGTGAAGSAHQIAYQQCPDCRRATQNGA
                     GREIDIASEVFERAACDARDLGSLDAPAPERATTTVTPRLRAQVFARDHHRCTVPGCR
                     SARNLDVHHIVPRARGGKHELWNLTLLCSGHHAALHDGLLELCGQAPYEIEVRWAYGP
                     PLPLGLGPEARQAMIRERIATIFEGRPVAIGPAMADPGTARPGWDGASAAPP"
     gene            2065..2700
                     /locus_tag="VF516_39295"
     CDS             2065..2700
                     /locus_tag="VF516_39295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113863.1"
                     /translation="MRGGSQRAAGRGHRRSTAVACAALLAASLACQRPAAGEFRDGCP
                     GCTWQRLGLHPQAAAQSTATGRALMTLCPWRGRLYIGYGDYQANTGPTAVTAWDPTRG
                     AFVTVHTSDTEAIYNFRPFGDALYAPATDRREHADYAVGEPWRDTQPVTSAHVYDMAT
                     LDGRDLGDHLVGQDAERVDITAAVEWLDHQLLGRHVVDGLISVRQSACRTR"
     gene            2821..3531
                     /locus_tag="VF516_39300"
     CDS             2821..3531
                     /locus_tag="VF516_39300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113864.1"
                     /translation="MDDALRVGFGERAAHLDEDLEHPLPFEWCLVVEHLVQALAVEEL
                     HHDVQRAPRRLIEVVHRDRVGIAQVAHHHDLTAKPRDQLVVPGHVGVQELECDLAAGR
                     KLYTTIHRAEHALADLRLDLVPIVGHAVEVQIPRLGTLLGGRAHRQLLRWVRLLRSQP
                     VAAHAAESSIRLERPVTGWAAHGDFDTGTGRVRRNARANAAAVAHANFVQLSERLSPT
                     MSLMPRIGNIGQRSRAAR"
BASE COUNT          590 a         1243 c         1298 g          548 t
ORIGIN      
        1 atcgagaata aattcctcat ctgtcactcc cagggcaaca cgtctctgcc gccgcgcccc
       61 acgcacggcg atcgactgca gatacggact gctggagcgg cagttacttc gaatccagcg
      121 aggcgcgtcg aaccgacggc atcgcatcgc ggcaagcgct gccctcgacg agcaagcgat
      181 ctgcccggtg gcatcccggc gtccatcgcc ggcaccgcga tcgcgctggc gggcgcgact
      241 ccagatcgat atcgccgggc cgccgatcgc agcgcgcggc tcgacgggag ggtcggatcg
      301 atcaccgagc aggcgatgga gcgcgcagac aaggcacgga aattctcagt ccaatgacga
      361 ttttgcttga cagcgcagga gtggtccgcg cgcagcgaat cgacaacctg cctgtgatcc
      421 tgcgcagcga gcgtcagatt gcgacaggag tgcaccattc tcgtggcgag ccaaaaacgc
      481 agccagggtc attttcgtgc aggaggtcgc agcaggatgc gtcgaaaaac cccgtgctac
      541 agaggacaca cacgcgaacc aggtatctgc gaaagggaac gtcgatgttg ctgcacagcg
      601 agagcaccga ggagcgagat ggacagacca tggaaccgtg gcgatcggag tggagcgaga
      661 tccatcgccg gctgcgcgag tacgcgcggc aacgcgcctc gctcgatgcc gccgaggcgt
      721 tcgacctggt gcgcgccgag cagctgaagc tccatttcct gtgcggcttc gccaccatgt
      781 acgagtacat ggagcgcgtg ctgggctacg gcccgcatgc ggcgcgcgag cggctgcggg
      841 tcgcgcgctc gctcgcgaag cttccacaga ccacggcggc gctggcccgc ggcgcgctga
      901 cgttctcggc cgcgcgcgag ctgacgcggg tggcgaccgc cgagacggaa ggccgctggc
      961 tgacggccgc cgagggcatg accgcggggc agatcgagcg cctggtcgcg ggccaccagc
     1021 ccggcgatca gccggaggat ccgacgcacc ccgacctgcg gtcgcgcgtg gtgcggctcg
     1081 agctgccgcc ggaggtctac gcgctgtggc ggcaggcgca cgcgatcgtc gccgccgagc
     1141 ggggcgcgga gatcagcgac gcggacttcg tggagtcgct gtgccgcgcg gtgatcgcac
     1201 cgggcaccgg cgccgcgggg tcggcgcatc agatcgccta ccagcaatgt ccggactgcc
     1261 ggcgcgcgac gcagaacggc gccggccggg agatcgacat cgcgtcggag gtcttcgagc
     1321 gggccgcgtg cgacgccagg gacctcggct cgctggatgc gccggcgccg gagcgcgcga
     1381 cgaccacggt gacgccgcgg ctgcgggcgc aggtgttcgc gcgcgaccat caccgctgca
     1441 cggtgcccgg ctgccggtcg gcccgcaacc tcgacgtcca ccacatcgtg cccagggccc
     1501 gcggtggcaa gcacgagctg tggaacctga cgctcctgtg cagcggccac cacgcggcgc
     1561 tgcacgatgg tctgctggag ctttgcggtc aggcgccgta cgagatcgag gtccgctggg
     1621 cgtacggccc gccgctgccg ctcgggctgg gtcccgaggc acggcaggcg atgatccgcg
     1681 agcgcatcgc gacgatcttc gagggacggc cggtggccat cgggcccgcg atggcggatc
     1741 ccgggaccgc gcgtcccggc tgggacggtg cttcggccgc tccgccgtga gaccggctct
     1801 cggcaagacg tctgccacgg cgcctccagc caggcaggcg acctggctcg tagcgtgcgc
     1861 gccgcggtgg gcttggactc gagcacggca gccggatctc cgccggggtt gacacgctgg
     1921 gtgtcgccct ccagcgcctc cagcgcgccg cgatccacgg gccggttggc ggtggatccg
     1981 gcgcacgcgc ctgcgacgat ccctccgcac gcgcgtgcga ccggatcgat tgacgcgcgg
     2041 gttcagccgc acgacgatgc aggcgtgcgc ggtggatcgc agcgggctgc aggtcggggc
     2101 cacaggcgat cgaccgcggt ggcgtgcgct gcgctgctcg ccgcgtcgct cgcgtgccag
     2161 cggccggccg ccggcgagtt ccgagacggg tgcccgggct gcacgtggca gcgcctcggg
     2221 ctgcacccgc aggccgctgc gcagtccacc gcgaccggcc gcgcgctgat gacgctgtgc
     2281 ccgtggcggg gccggctgta catcgggtac ggcgactacc aggcgaacac cgggccgacg
     2341 gccgtgacgg cgtgggatcc cacacgtggt gccttcgtca cggtccacac ctcggacacc
     2401 gaggcgatct acaactttcg tccgtttggc gacgcgctct acgcgccggc gaccgaccgg
     2461 agggagcacg ccgactacgc cgtcggcgag ccgtggcggg acacccagcc ggtgacctcc
     2521 gcgcatgtgt atgacatggc gacgctcgat ggccgcgacc tcggtgatca tctcgtaggc
     2581 caggacgccg agcgcgtaga tatcactgcg gccgtcgagt ggcttgacca tcagctgctc
     2641 gggagacatg tagtcgacgg cctgatatcc gtacgacaga gcgcctgtcg tactcgatag
     2701 cgagtacaag ccgcgctgtc cgcgaccaaa aacgttggca atgccgtagt cgaggatctt
     2761 gacgaactcg gggttgcccg agcgcgactc gaggtggatg ttctcgggct tgagatcgca
     2821 gtggacgatg ccctgcgcgt gggcttcggc gagcgagctg cacatctcga tgaggatctt
     2881 gaacacccgc ttccattcga gtggtgcctc gtcgtggaac acctggtgca ggctcttgcc
     2941 gtcgaggagc tccatcacga tgtacagcgc gccccgcggc gtctgatcga agtcgtacat
     3001 cgtgatcgtg tgggcatcgc gcaggttgca caccaccatg acctcacggc gaagccgcgc
     3061 gaccagctcg tcgtccctgg ccacgtcggc gtgcaagagc ttgagtgcga ccttgcggcc
     3121 ggtcgcaagt tgtacaccac gatacaccgc gccgaacacg ccctcgccga tcttcgactc
     3181 gatcttgtac cgatcgttgg gcatgccgtc gaggtgcaga tcccgaggct tgggaccctg
     3241 ctcgggggcc gtgcacatcg ccagttgctg cgctgggtcc gtctcctgcg atcgcaaccg
     3301 gtggccgcac acgccgcaga atcgagcatt cggctcgagc ggccggtgac aggatgggca
     3361 gctcatgggg atttcgacac cgggaccggc cgcgtccgta gaaatgcacg agcaaacgcc
     3421 gccgccgttg cacacgcgaa cttcgtccag ctatccgaac gtttgagtcc gacaatgtcg
     3481 ctgatgccgc ggatcggcaa catcgggcaa cgctcgcgtg cggcgcgata gacgcccccc
     3541 gactccatct cgatggcgag caggttgcgg gccgtggcca gccacgggaa cagggtcgtc
     3601 gggtccttca ccagccggtc actcgacgcg atcggtccca ggatgtacct cggggggcgc
     3661 cgctgcgcgt gcttgccat
//
LOCUS       DASVOR010002120         3595 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_490469,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002120 DASVOR010000000
VERSION     DASVOR010002120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3595
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490469"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1742
                     /gene="ligD"
                     /locus_tag="VF516_39305"
     CDS             <1..1742
                     /gene="ligD"
                     /locus_tag="VF516_39305"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HEX8113865.1"
                     /translation="HRMTAATRPRRTPGTPDASLRVMAGAAAAKLAKYRSKRDFTRTA
                     EPRGEAVASPGHRFVIQQHAARRMHYDFRLELDGVLLSWSVPKGPSLSPRDRRLAVRT
                     EDHPLDYADFEGVIPQGEYGGGAVVVWDRGTWQPEGDARDGMAQGKLTFALHGEKLHG
                     RWHLVRTRGGERGENWLLFKGRDDAASDRIDIVASQPASVVSGRTVEEIAAAPDRVWH
                     SNKVAEPTVAARRAARIAASRPKRPSPDTGREIERDVDRRNVHALVEQLPLGFPLSNL
                     DKVLYPDQGITKGELIAYLAVVADHALPHIANRPLTLVRCPEGIAHRGRGRTCFFQKH
                     ILPGSPAAIEPVPIVEDTGETADYMAVHDLPGLAALAQLGTLEIHTWGCHADRPEQPD
                     LIVFDLDPDVGLGWERVVLAAFELRRRLHDVGLASFVKTTGGKGLHVVAPIARGVGWD
                     DFKAFSKALVEGMERDEPDAYTTNPIKLRRKGKIFLDYLRNGRGATFIAPYSPRARAG
                     APVAIPITWDELAHGVDPAAFTTATVPRRLVTQKIDPWQDVPRLKQSITAAAWRAVGL
                     PPPHAGARAHRKR"
     gene            complement(1886..2455)
                     /locus_tag="VF516_39310"
     CDS             complement(1886..2455)
                     /locus_tag="VF516_39310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113866.1"
                     /translation="MQGWDRYLSVRFLGQGGMGQVFLAVDPRLRREVAIKFVRGDNPE
                     HVRRLIAEARTQARVNHEHVRELERDSLHELLRRELGARCHRRPGDHRRAAGVDVQED
                     AARGARGEAQRLATDERIAERRPEGAAGPREVGGVARHADRAAGEGEHDALASAAEDR
                     MRDDGQRDGEADIGGTAARADGRGDIPDR"
     gene            complement(2431..2733)
                     /locus_tag="VF516_39315"
     CDS             complement(2431..2733)
                     /locus_tag="VF516_39315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113867.1"
                     /translation="MADRLDAEMRIVLAEGLLPRDEAAALRQEALDARQSPLALLVER
                     GRLSERSYRSLVALAFDDTSVANLDPSTSTSTHRLPAHPHPRRMHRASPCRAGIAT"
     gene            2874..3212
                     /locus_tag="VF516_39320"
     CDS             2874..3212
                     /locus_tag="VF516_39320"
                     /inference="COORDINATES: protein motif:HMM:NF019009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system CcdA family
                     antitoxin"
                     /protein_id="HEX8113868.1"
                     /translation="MTSAEASTRTFAGRARCGAPGCMSPELRIFCAQMSATRKTRKIP
                     TNLSLRGDLVRRAKKLGLKLSSVVDAALEQAVKEAEAKAWLDENRDAIVEYNALVAKR
                     GVFSAGRRQF"
     gene            3212..3520
                     /locus_tag="VF516_39325"
     CDS             3212..3520
                     /locus_tag="VF516_39325"
                     /inference="COORDINATES: protein motif:HMM:NF013965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdB family protein"
                     /protein_id="HEX8113869.1"
                     /translation="MPQFDVHKDRKGALYPLLLDVQADLLSHLDTRVVVPLAPRRRYP
                     ARPMRIVNPVLVIHGVEYVAVFQELAAILSSELGDVVGSVAAHRTEVVAALDFLFTGS
                     "
BASE COUNT          509 a         1333 c         1265 g          488 t
ORIGIN      
        1 cccaccggat gacggccgcg acccgcccgc gccggacccc tggcaccccg gatgcatcac
       61 tccgcgtcat ggccggagct gcagctgcca agctcgcgaa gtaccgatcg aagcgagact
      121 tcacgcggac ggcggagccc cggggcgagg ccgtcgcgag ccccggccat cgcttcgtca
      181 tccagcagca cgccgcccgc cggatgcatt acgacttccg gctggagctc gacggcgtgc
      241 tgctgtcgtg gtcggtgccc aaggggccga gcctgtcgcc ccgggaccgc cggctggcgg
      301 tgcgcaccga ggaccacccg ctcgactacg ccgacttcga gggcgtcatc ccccagggcg
      361 agtacggcgg cggcgccgtc gtggtctggg accgcggcac ctggcagccc gagggcgacg
      421 cgcgcgacgg catggcccag ggcaagctga ccttcgcgct gcacggcgag aagctgcacg
      481 gccgctggca cctggtgcgg acccgcggcg gcgagcgcgg cgagaactgg ctcctgttca
      541 agggccgcga cgacgccgcg agcgaccgga tcgacatcgt cgccagccag ccggcgagcg
      601 tggtgtcggg ccgcaccgtc gaggagatcg ccgccgcgcc cgaccgggtg tggcacagca
      661 acaaggtcgc cgagccgacg gtcgccgccc gccgcgccgc ccggatcgcc gcctcgcgac
      721 cgaagcggcc gtcgccggac accgggcgcg agatcgagcg cgacgtcgac cgtcgcaacg
      781 tgcacgcgct ggtcgagcag ctgccgctcg gcttcccgct cagcaacctc gacaaggtgc
      841 tgtaccccga tcagggcatc accaagggcg agctgatcgc gtacctcgcg gtggtcgccg
      901 atcacgcgct gccgcacatc gcgaaccgcc cgctgaccct ggtgcgctgt cccgagggca
      961 tcgcgcaccg cggccgcggc cggacctgct tcttccagaa gcacatcctg cccggctcgc
     1021 cggcggcgat cgagccggtg ccgatcgtcg aggacaccgg cgagaccgcc gactacatgg
     1081 cggtccacga cctgcccggc ctcgccgcgc tcgcccagct cggcacgctg gagatccaca
     1141 cctggggctg ccacgccgac cggcccgagc agcccgacct gatcgtgttc gacctcgatc
     1201 ccgacgtcgg cctgggctgg gagcgcgtcg tgctcgcggc gttcgagctc cgccgccggc
     1261 tgcacgacgt cggcctggcc agcttcgtca agaccaccgg cggcaagggc ctgcacgtcg
     1321 tcgcgccgat cgcgcgcggc gtcggctggg acgacttcaa ggcgttcagc aaggcgctgg
     1381 tcgaggggat ggagcgcgac gagcccgacg cttacaccac caacccgatc aagctgcggc
     1441 gcaagggcaa gatcttcctc gactacctgc gcaacggccg cggcgcgacg ttcatcgcgc
     1501 cgtactcgcc ccgcgcgcgc gccggcgcgc cggtcgccat cccgatcacc tgggacgagc
     1561 tcgcgcacgg cgtcgacccc gccgccttca ccacggcgac ggtgccgcgc cggctcgtca
     1621 cgcagaagat cgacccgtgg caggatgtcc cccggctcaa gcagtcgatc accgcggctg
     1681 cgtggcgcgc ggtcgggctc ccgccgcctc acgccggagc ccgagctcac cgcaagcgat
     1741 gatcacctgc atggcatcga ccgcgcggcc cgcggtgcgg cgcgccgacg ccttccctcg
     1801 ccgcgactga cctgcgaaca tcaccggcgc tgcctcgaac ccgcggcacg gaacgaggcg
     1861 cgcgtgacga ccgcgcatgg ccaggtcagc gatcagggat gtctccacgg ccgtcggcgc
     1921 gggcggcggt gccgccgatg tccgcctcgc cgtcgcgttg accatcgtct cgcatccggt
     1981 cctcggccgc gctggcgagc gcgtcgtgct ccccctcgcc ggcggcgcgg tcggcgtgtc
     2041 gcgcaacgcc cccgacttcg cgtggcccgg ccgcaccttc ggggcgccgc tcggcgatcc
     2101 gctcatcggt cgcaagccgc tgcgcttcgc cgcgggctcc ccgggcagcg tcctcctgca
     2161 cgtcgacgcc ggcagcacgc cggtgatcgc cggggcgccg gtgacagcga gcgccgagct
     2221 cgcggcggag cagctcgtgc agggagtccc gctcgagctc gcgcacgtgc tcgtggttga
     2281 cccgcgcctg ggtgcgcgcc tcggcgatca gccgccggac gtgctcgggg ttgtcgccgc
     2341 gcacgaactt gatcgcgacc tcgcggcgca gccgcggatc gacggcgagg aacacctggc
     2401 ccatcccgcc ctggcccaga aagcgcacgc tcaggtagcg atcccagccc tgcacgggga
     2461 agcgcggtgc atccgccggg ggtgcgggtg tgcgggtagc cggtgggtcg aggtcgaggt
     2521 gctcgggtcg aggttggcga ccgaggtatc gtcgaacgcg agggccacga gcgatcggta
     2581 ggagcgctcg gacagccggc cgcgctcgac gagcaacgcc agcgggctct gccgggcatc
     2641 gagcgcctcc tggcgcagcg ccgccgcctc gtcccgcggc agcaacccct ccgcgagcac
     2701 gatccgcatc tcggcatcga gacggtcggc catcaaatca aggctacgtt cgatgcgtca
     2761 tccggcgcga cgagtcgagc tggagcgatt cgaggcacgc agcgcgtcgc gatgaacccc
     2821 gcagggtggt cgtacgtacg tgatcgtatg cccgtcggct tcccgatccg gggatgacct
     2881 cggccgaggc ctcgacgcgg acgttcgctg gccgagctcg gtgcggggcg cccggttgca
     2941 tgtccccgga gttgcgcata ttctgtgcgc agatgagcgc aacacggaaa acgcgcaaga
     3001 ttcctacgaa cctgtcgctt cgcggggacc tcgtccggcg cgccaagaag ctcgggctca
     3061 agctgtcgtc cgtggtcgat gcggccctgg agcaggccgt caaggaggca gaggcgaagg
     3121 cgtggctcga cgagaaccgt gacgcgatcg tcgagtacaa tgccctggtt gcgaagcgcg
     3181 gcgtgttcag cgccggtcgg cggcagttct gatgccccag ttcgacgtcc acaaggatcg
     3241 caagggcgcc ttgtatccgt tgctcctcga cgtccaggcg gatctgctgt cgcacctcga
     3301 cacgcgcgtc gtggtacccc tcgcgccaag gcggcgatat ccggccaggc cgatgcggat
     3361 cgtcaacccg gtgctggtga tccatggcgt cgagtacgtc gccgtgttcc aggaactggc
     3421 cgcgatcttg tcctcggagc tcggcgacgt ggtcggctcc gtcgccgcgc atcggaccga
     3481 ggtcgtcgcc gcgctcgact tcctgttcac gggcagctga tggcctggct gggtcaggcg
     3541 cggcagctcg gcggccggat tcacacgccg cggcatcacg gcgaaccgcc cggcg
//
LOCUS       DASVOR010002121         2016 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_460894,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002121 DASVOR010000000
VERSION     DASVOR010002121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_460894"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1921)
                     /locus_tag="VF516_39330"
     CDS             complement(<1..1921)
                     /locus_tag="VF516_39330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113870.1"
                     /translation="MSELMRTAWLDFLHAETAAHPVLLVLDDLHWGDFGTVRFIDVGL
                     RDLSQQPWMVLALARPEVFEVFPRLWAERQNVQELRLKELGRRAGERLVRQVLGDTVG
                     SDTVERLIKQADGNAFYLEELIRAVAEGKDAALPETVLAMVETRLARLPSKARRVLRA
                     ASVFGEVCWESGVVTLLDDAMPPAAVAEWLAMLIDQEVLVVRPDSRFPGERELAFRHA
                     LLREGAYATLTADDERVAHRLAGEWLERHGEADPMVLAGHAQRGGDKVRAAEFYLRAG
                     QQAVHVLDNEAAIARANLGLSCDPPPELRIALLGIRCNASHRLQRHALADAEELVRSA
                     PRGSIPWAQGMASYQMATLITGREEDFQASLALLAEVTPDPDAMHWISVLFLTSTVVL
                     DNFGRVAQATALEEPFLALASRPGDQVPLARFWWNFSVGLRASHAHDDPWNALVHSDA
                     IQPISDLIGGDVYFLVMQLLRGMNLWYLGAHARAVKLLESIPGADAALGEGNSFRRLF
                     LSWLYADRGALDEARALAIEVRAYCRAHHDRNGEARGTWVLAEVLRRIGDLEGAEREI
                     QAAQAMGMPYDHPGKLATLAVLRLVQGRPADALAVAEDAMVRCAAMGGGCGLFRTAAV
                     RLVHAEALHATGAHDA"
BASE COUNT          294 a          747 c          715 g          260 t
ORIGIN      
        1 cggcgtcgtg ggcgccggtc gcgtggagcg cctcggcgtg gacgaggcgc accgcggcgg
       61 tgcggaacag gccgcacccg ccccccatgg ccgcgcaccg caccattgca tcctcggcga
      121 ccgcgagggc gtcggccggg cggccctgga cgaggcggag caccgccagc gtcgccagct
      181 tgccgggatg atcgtacggc attcccatgg cctgcgcagc ctggatctcg cgttcggcgc
      241 cctccaggtc gccgatccgg cgcagcacct ccgcgagcac ccaggtgcct cgggcctctc
      301 cattgcggtc gtgatgcgcg cggcagtacg cgcggacctc gatcgccagc gcgcgcgcct
      361 cgtcgagcgc accacggtcg gcgtacagcc aggacaggaa gagccggcga aacgagttcc
      421 cctcgcccag cgcggcgtcc gccccgggga tggattcgag cagcttcacc gcgcgggcgt
      481 gcgcgcccag gtaccacagg ttcatgccgc gcagcagctg catgacgaga aaatagacgt
      541 cgcccccgat caggtcggag atcggctgga tcgcatcgct gtgaaccagc gcgttccacg
      601 ggtcatcgtg cgcatgggac gctcgcaggc cgacggagaa gttccaccag aaccgcgcga
      661 gcggcacctg atctcccggc cggctggcga gcgcgaggaa cggctcctcc agcgcggtgg
      721 cctgcgcgac acgcccgaag ttatcgagca cgacggtgct ggtgaggaac agcaccgata
      781 tccagtgcat cgcgtccggg tccggggtga cctcggcgag cagcgcgagc gacgcctgga
      841 agtcttcttc ccggccggtg atcagcgtgg ccatctgata cgaggccatg ccctgcgccc
      901 agggaatgga gccccggggc gccgagcgca ccagctcctc ggcgtcggcg agcgcgtggc
      961 gctgcaggcg gtgcgacgcg ttgcagcgga tgccgagcag ggcgatccgg agctcgggcg
     1021 gcggatcgca gctcagaccg aggttggcgc gggcgatcgc ggcttcatta tcgagcacgt
     1081 gcaccgcctg ctgccccgcc cggaggtaga attcggcggc gcgcaccttg tccccgccgc
     1141 gctgggcgtg ccccgccagg accatgggat cagcctcgcc gtggcgctcg agccactcgc
     1201 cggcgagacg atgcgcgacc cgctcatcgt cggcggtcag cgtggcatag gcgccctcgc
     1261 ggagcagcgc gtggcggaac gcgagctcgc gctcgccggg gaaccggctg tccggccgca
     1321 cgaccagcac ctcctggtcg atcagcatcg ccagccactc ggcgaccgcc gccggcggca
     1381 tggcgtcgtc gagcagcgtg accacgccgc tctcccagca cacctcgccg aacacgctgg
     1441 cggcgcgcag cacccgccgc gccttgctcg gcagccgcgc cagccgggtc tcgaccatgg
     1501 ccagcacggt ctccggcagc gccgcatcct tgccctcggc gaccgcgcgg atcagctcct
     1561 cgaggtagaa cgcgttgccg tcggcctgct tgatcagccg ctcgaccgtg tcgctgccga
     1621 cggtatcgcc gaggacctgg cggaccagcc gctcgcccgc ccggcggccg agctccttga
     1681 gacggagctc ctgaacgttc tggcgctccg cccacagccg gggaaacacc tcgaacacct
     1741 cggggcgggc cagcgcgagc accatccacg gctgctggct cagatcgcgc agcccgacgt
     1801 cgatgaaccg cacggtgccg aaatcgcccc agtgcaggtc gtcgagcacg agcaggaccg
     1861 ggtgcgcggc ggtctcggcg tggaggaagt ccagccaggc cgtgcgcatc agctcgctca
     1921 tgagctgggc gtccaggcgc gcggcccgca gcgccgcgct cgccgcgccg gcggcgctgt
     1981 tgtcgtcggg catcggcgca ccggcgagct cgccga
//
LOCUS       DASVOR010002122         4575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_81298,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002122 DASVOR010000000
VERSION     DASVOR010002122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81298"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            798..1595
                     /locus_tag="VF516_39335"
     CDS             798..1595
                     /locus_tag="VF516_39335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase family protein"
                     /protein_id="HEX8113871.1"
                     /translation="MTEIHKYPRTRHLEGSRLQPGDADLDQVPLDALRGRYCVVEEKL
                     DGANAGISLDADRRLRLQSRGHVLIGGAREKHWDLFKRWARAHATALGERATGGLTIF
                     GEWLYAKHTIFYDQLPHYFLEFDIRDATGAFWSTERRLAHLAGSPIVSVPVLWHGVVT
                     DPRRLPELVGHALYKSPRWRERLRQAATDANVDPDRTALETDPEPTAEGLYIKVEDRA
                     QGIVVDRYKWLRASFLSAVLDSGSHWLARPIVANQLADGVDLFAVAP"
     gene            1592..2749
                     /locus_tag="VF516_39340"
     CDS             1592..2749
                     /locus_tag="VF516_39340"
                     /inference="COORDINATES: protein motif:HMM:NF025052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8113872.1"
                     /translation="MIAPAPAAPIDATAIAAFLEPIAPAAELAATPQDAALHAEGDVW
                     THTQMALQALVDGPGYAALGPLARRIVATAVVLHDIGKPATTRHEAGKLTSRGHSARG
                     EILARAALWHLGAPFAVREHVCALIRHHQIPFFGLDKPADEAIRLARRMSLVLRHDWL
                     TAVADADARGRRCADPADQTRIIDACALWAEHARELAILDRAQPFASSHSRRVWCESA
                     TRSLDVAAYDNTTCEVIVMAGLPASGKDSWLAAHRPGIPVISLDELRTELAIDPNDAQ
                     APVLKAAREQARDHLRAGTPFAWNATNLSRSLRTQLLDLLRSYRARTHVVYCEASPVE
                     QDRRNRARRDPVPRTAIARMIECWTVPDPTEAHEITYVVPSSSDDIVWPPA"
     gene            2895..3668
                     /locus_tag="VF516_39345"
     CDS             2895..3668
                     /locus_tag="VF516_39345"
                     /inference="COORDINATES: protein motif:HMM:NF012238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113873.1"
                     /translation="MLAGATALSLIGGCAGSTAPPPEQHLGEATQHLDGDQCVYFDVD
                     GKDTICHHTGSVSHPFTLIKISDQACIDGHAAHAEDYIASLDPASSLFDPICNGQGCL
                     PTAAPCDGTLDCCTGSCMDGTCVDPCSPNPCQNGGTCASSGSSFTCTCTGGFTGTTCE
                     IAPGPAIGCHRNAAGNQSLNDLKYLGPIDTLNNVQGFQSTDGTCSGRSLPGSFALISA
                     ANASDAEAKCQSLFGFDAVDASIWSGLAGFWLCAPGSAG"
     gene            4272..>4575
                     /locus_tag="VF516_39350"
     CDS             4272..>4575
                     /locus_tag="VF516_39350"
                     /inference="COORDINATES: protein motif:HMM:NF015038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8113874.1"
                     /translation="MLAQLVTAKNSDAVPLERQSKILARGGARIASPTLGDWYAGAAD
                     LAQPLWKALRDDTLGRYLISRLGPEAGTIPFGWTSRATDDRSDTGRGWRRLEAPRWT"
BASE COUNT          703 a         1651 c         1472 g          749 t
ORIGIN      
        1 gatcgcggtg ccgcaccagg tccgcgccga gcaacctcga cccgctcgac gccagcgagg
       61 ccgggccagc ggctggcagc tccgcggccg gcagctcggc cgccgcgctc ccatggcgag
      121 acagtcggcc ccgcggtccc tggtgcgatc ctcgattctt gtaggtcgtc ccgcccatct
      181 gtctgtctcg cgaattgacg cgtgcaggct tttttgtgac acccctttcg ttggatatga
      241 tttcgtgttt tgtagtgatg tggccagtcg tccaagaaag agcacgtggt cggaagccgc
      301 cgtgatggcg atcgggcggg ccctggaact aaaaaataga ttccttcggt gcttttcggg
      361 gccagatatc tgcacatcaa cgcaagccgt cgaggacttc gccgctatcg ccaacggttc
      421 tctcgagact gaaggcatgc tgcgattcgc tcaccatacg tcccaatgtt cctcgtgcag
      481 agacatgcta gtgacgctga ttatggcaac tggcttgcgc cgcgtgctcc gcgctgggct
      541 tcaggaactt gtaagaaccc gacctcgagg tcaggcatga ataccaacac tcacgccgcg
      601 tcgaactgcc cacgctcaat cgcggcaccg ccgaccgtgc ggccgtccgc gactgacctg
      661 tcgccgagct tgacgtccct gcagcgctgc cgcagccagc agctcgccgc cgcgtcgccg
      721 cagccagcag cggcccacgg cgccaccggc cgctggcctg tcgcgcgggt cgtacgaccc
      781 atggggcgga cggtcgaatg accgagatcc acaagtatcc gcgcacccgg caccttgagg
      841 gctcacggct gcagcccggc gatgccgacc tcgatcaggt gccgctcgac gcgctgcgcg
      901 gacggtactg cgtcgtcgag gagaagctcg atggtgccaa cgccggcatc tcgctcgatg
      961 ccgaccggcg gcttcgtctc cagagccgcg gacacgtcct gatcggtggc gcacgcgaga
     1021 agcactggga tctgttcaag cgctgggcgc gcgcacacgc gaccgcactc ggcgagcgtg
     1081 caaccggcgg cctcaccatc ttcggcgaat ggctgtatgc caagcacacg atcttctatg
     1141 accagctacc acactacttc ctggagttcg atatccgcga cgccaccggc gcgttctggt
     1201 cgaccgagcg acgcctcgcc caccttgccg gctcgccgat tgtctcggtt cccgtcctgt
     1261 ggcacggcgt tgtgaccgat ccgcgccgcc tcccggagct cgtcgggcac gcactctaca
     1321 agtcaccgcg ctggcgcgag cgcctgcgcc aggctgcaac ggacgccaat gttgatcctg
     1381 atcgcaccgc cctggagacc gatccggaac ccaccgccga gggcctctac atcaaggtcg
     1441 aggaccgcgc gcaaggaatc gtcgtcgatc gctacaagtg gctccgcgca tcgttcctgt
     1501 ctgccgtgct tgactccggg agccactggc tggcgcgccc gatcgtggcg aaccagcttg
     1561 ccgatggcgt cgacctgttc gcggtggccc cgtgatcgcc cccgctccgg cggccccgat
     1621 cgacgcgacc gcgatcgccg cattcctcga gccgatcgcg ccggccgccg agctcgccgc
     1681 gacgccccag gacgctgcac tgcacgccga gggcgacgtg tggacccaca cgcagatggc
     1741 gctccaggcg ctggtcgacg ggcccggcta tgccgcgctc ggaccgcttg cccgccggat
     1801 cgtcgccacc gcggtcgtcc tccacgacat cggcaagccg gcgacgaccc gccatgaggc
     1861 cggcaagctg acctcgcgcg gccactccgc ccgcggcgag atcctggcac gcgctgcgct
     1921 gtggcacctc ggtgcgccgt tcgccgtccg cgagcacgtg tgcgcgctga tccgtcacca
     1981 tcagatccca ttcttcgggc tcgacaagcc agctgacgaa gccatccgcc tcgctcgccg
     2041 gatgtcgctg gtgttgcgcc atgactggct caccgcggtt gccgacgccg atgcgcgcgg
     2101 gcggcgctgc gccgatcccg cggatcagac ccgcatcatc gatgcctgcg cgctgtgggc
     2161 cgaacacgcg cgtgagctcg cgatcctcga ccgcgcgcag ccgtttgcgt cgtcgcactc
     2221 ccgccgcgtg tggtgtgaat ccgcgacgcg gtccctcgac gtggccgcgt acgacaacac
     2281 cacgtgcgag gtcatcgtga tggccggctt gccggcctcg ggcaaggaca gctggctcgc
     2341 cgcgcaccgc ccaggcatcc cggtgatctc gctcgacgag ctccgtaccg agctcgcgat
     2401 cgatcccaat gacgcccagg cccccgtgct caaggccgcg cgcgagcagg cgcgcgacca
     2461 tctccgtgca gggaccccgt tcgcatggaa cgccacgaac ctgtcacgtt ccttgcgcac
     2521 ccagctgctc gacctgctcc gctcgtaccg ggcgcgcacc cacgtcgtgt actgcgaggc
     2581 ctcgcccgtc gagcaggacc ggcgcaaccg agcgcgccgc gaccccgtgc cgcgcaccgc
     2641 catcgcccgg atgatcgagt gctggaccgt gccggatccc accgaggcac acgagatcac
     2701 ctacgtcgta ccgtccagct ctgacgacat cgtttggcca cctgcatgag ccccggcagc
     2761 cgccgctcgg cgccggttcg gcgtgttcgc accagctgca cggatcacgc cggtctcacc
     2821 cgggagcggt gctcgcccag aggttcacct gctccgggca gaactatcgc tctcaggtgg
     2881 tcgcttcttg cgtcttgctc gcaggcgcca cggccctctc gcttatcggc gggtgcgccg
     2941 gatccacggc accgcccccc gaacagcacc tcggcgaggc cacccagcac ctcgacgggg
     3001 atcagtgcgt ctacttcgac gtcgacggaa aggacaccat ctgccaccac acgggctccg
     3061 tgagccatcc gttcacgctc atcaagatca gcgaccaggc ttgcatcgac ggccacgccg
     3121 cccacgccga ggactacatc gccagcctcg atccagcgtc ctcgctgttc gatccgatct
     3181 gtaacggcca gggctgcctc ccgaccgccg cgccgtgcga cggcacgctg gattgctgca
     3241 ccgggagctg catggacggt acgtgcgtcg atccatgctc gcccaacccg tgccagaacg
     3301 gtggcacctg cgcgtcgagc ggcagctctt tcacctgtac ctgcaccggc gggttcaccg
     3361 ggaccacctg cgagatcgcg cccggacccg ccatcggctg ccaccgaaat gcggctggta
     3421 accagagcct gaatgacttg aagtatctcg gaccgatcga caccctcaac aatgtccagg
     3481 gctttcaatc aacggacggg acctgcagtg ggcgctcact cccgggtagt ttcgcgctga
     3541 tttcagctgc caacgcgtcg gacgcggaag ccaagtgcca gtccctcttt ggatttgatg
     3601 cagtagatgc atcgatctgg agtggcctgg cagggttctg gctgtgcgct ccgggttcag
     3661 ctgggtgacc gcgcttctgg ccggtgtacg gcggcagtgg tcgggggcga caacacgacg
     3721 ctgacgatca cgtgtcccgc cggcatcaaa cgtcgctcgg accttcccgg cggacggttg
     3781 tgtcctttgt gaaacacttg cccctgcaat gacggagtcg aggtccgcag atcgcgttga
     3841 ctgccgcgtg acgggatggg atcgctgcag cgtcgatccc agcgaacacc ggcggctgcc
     3901 ccgaggggat gattcaggaa cactcgacgg ccttcccagt gcgttccgcc gcgtcgggat
     3961 tggggggggc tgggcggcgc cgaagatcga ctacggcgcc ggccgggcgt gctccgccga
     4021 ccggactgga tgccacgagc acgtcggccg gactggacgc cgcggtcgtc gtgatcgggg
     4081 gcggagccga gttgggcgcc gcgctcgcgc cggccgagtt gcgaaacgac tcgagcgcgg
     4141 ccggtttcgg ttgccgtggg attcgcccgt ggcgggtgcg gcggtggaga tggaggcagc
     4201 cgagctcgcg ctgatcctcg aaggcgccga tcactccgcc gccgatcgag agcgcgcggc
     4261 ccggcccgag catgctcgcg cagcttgtga cggcgaagaa ctccgatgct gtgccgctgg
     4321 agcgccagag caagatcctc gcgcgcggcg gcgcgcggat cgcctcgccg acactcggcg
     4381 attggtacgc gggcgccgcg gatctcgccc agccgttgtg gaaggcgctc cgcgacgaca
     4441 ccctcggtcg ctacttgatc agccggctgg ggccggaggc cggcaccata ccgttcggtt
     4501 ggacgtcgcg cgccaccgac gaccgctccg acaccggccg cggttggcgc cggctggagg
     4561 ctccccgttg gactc
//
LOCUS       DASVOR010002123         3811 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401871,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002123 DASVOR010000000
VERSION     DASVOR010002123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401871"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..624
                     /locus_tag="VF516_39355"
     CDS             163..624
                     /locus_tag="VF516_39355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113875.1"
                     /translation="MHTVLRWIVMAGCLWLLGGVATAGNPLARPARRDARAHLDRGNR
                     LYNTRSFEEAIVEYKAGALIEPATVFDYNLAQSYRQLGKYKEAIWHYERFLNDGQPAG
                     ELLDAVNAFLEEMRVQLAGRAPRRGSQGPTGLDGDSPASAWLRWAPLDPCS"
     gene            615..866
                     /locus_tag="VF516_39360"
     CDS             615..866
                     /locus_tag="VF516_39360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113876.1"
                     /translation="MLLSAGHLSDRANTEPDTLRRNQLHEQARNRSTVGAILGIGGIG
                     LTAVGVVKLVLHARETTRPTAARLDVGITSNGAFVVGNF"
     gene            complement(1849..2721)
                     /locus_tag="VF516_39365"
     CDS             complement(1849..2721)
                     /locus_tag="VF516_39365"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF005537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEX8113877.1"
                     /translation="MLVLIALPGGDAHARELATRLGARLLVPEIRQFPDGEIYVRIDG
                     DCRGDVAIVGNLSADSFLRVAFLAGTARELGASRVGLVAPYLAYMRQDSRFKPGEGIT
                     ARYFARLVSGTVDWLVTVDPHLHRLDSLAGVYTIPTTIARAAPAIARWITDEVQRPVL
                     IGPDAESVQWVSAVAERCGAPFVILEKTRRGDRDVSITAPDGAWNGHTPVVIDDIVST
                     GRTMIEATRQLRAAGSAPPVCVAIHAVFADALADALLGAGARGIVTCDTIPHTTNRIC
                     VAESLADAVRQRLV"
     gene            complement(2876..>3811)
                     /locus_tag="VF516_39370"
     CDS             complement(2876..>3811)
                     /locus_tag="VF516_39370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113878.1"
                     /translation="RARPAPRPTTGRASGPPPPRPAVDQPALAPGAPARPPARPPATP
                     PSTSPGHRHRARLSVSPRSHLARSHVEDDGDGRLDKQEDTVLGGKHFRIKTARGAVHV
                     WVPPDYDRETAGTVIYVHGYYTDADGAWREHELARQFKASRQNAMFIVPDAPAGNDDE
                     VQWPALKDLRRAVTRANIHLPDGPVIVIGHSGAFRTVMQWVDHRLVDQIVLLDALYAG
                     ETAFDEFIASGKRADDHKLIVVAASTAEESSSFARRYKFVVARERMPPAVGDFTRRER
                     GAKLLYIHSQFEHMAIVTSGKVIPLLLRVTPLKAL"
BASE COUNT          615 a         1227 c         1383 g          586 t
ORIGIN      
        1 gtcgcgtcga gcagggtcgc tgacacaagc tgcagcccgc gtgtctcagg cgcgcagccg
       61 gcgtcacggg tccgcggccg cccggccgtt ccgcccaggg gcgggcctgg gaaacaccgt
      121 ttcgatccca atgcaattgg cgggcaggag ggaaactgat cgatgcatac cgtcctgcga
      181 tggatcgtga tggctggttg tttgtggctc ctcggcggtg tcgccacggc gggcaatcca
      241 ctcgcgcgcc cggcgaggcg cgacgcgcgc gcgcacctgg accgcgggaa ccgcctctac
      301 aacacgcgat cattcgagga ggcaatcgtc gaatacaaag caggcgctct catcgagccg
      361 gcgaccgtct ttgactacaa tctcgcgcag tcctatcggc agctcgggaa gtacaaggaa
      421 gcgatttggc attacgagcg tttcctgaac gatggccagc ctgcaggaga gctcctcgac
      481 gccgtcaacg cgttcctcga ggagatgcgc gttcagctag ccggccgagc accacgacga
      541 ggctcccagg gaccgactgg gttggatggg gactcaccgg cgtcggcgtg gctgcgatgg
      601 gcaccgctgg acccgtgctc ctgagcgcag ggcatctgag cgaccgggcc aacacggagc
      661 cagatacact gcgacggaac cagctgcacg agcaggcgcg caaccgcagc accgtcggcg
      721 ccatcctagg catcggtggg atcgggctca cggcggtggg cgtcgtgaag ctcgtgctcc
      781 acgctcgtga aacaacgcgc cccaccgcgg cgagactgga cgtcggcatc acgagcaatg
      841 gcgcgttcgt cgtcggcaac ttctgagcgc cgcgctcatc gtgcaccaac gagccgaacg
      901 gcctctgtga agctgatggc aaactatggt gaagcgtcac cgaggtcgac aacatcgtcg
      961 tcgagcgtcg cgcggatggt acgctgcagc ctgtgattct ggagcaggta aaggccggcg
     1021 ctgcccacaa cccctcggaa gagcgacaat agagagctga gcgccgacgc acggggcgcc
     1081 agcctgggca acactgcctc gatcgaggtg gccgacccga gccgaatggg ctatctcacg
     1141 gcgcgcatcg gcagccaacg cccaccagat ggcacttcgc gtgttcgatg tttggcgcgt
     1201 tgttcctcga gcgccaactc atgtgaccac gacaacagag agccgcgtac caacttgacc
     1261 tcgcaccggt tgtgtcgacg acgctcgccc tacgctgaga gatagaacat gcgtcagaac
     1321 ttcttacttc ttgctgtggt tgccacgatc accggatgtg acaaccgact cgccgtccag
     1381 tgctaatgga attgactgct taggtgatgg aaacctcggc tgtgctgtga cggacttgat
     1441 caacgttggc accaagcctg caggagatgc tcgttggggg cattccgata tggtcggtaa
     1501 cgtagatgaa atggtactag atgaatatgc aacgtacatg accccgtgca acgactgtgc
     1561 ggcgattatt ccaacgggga gcccaattac gcgcggcggg gaatttcatc tagccgctcc
     1621 cgaagaccgc ttcagaacgg gcagaaggcg ggggggctca ttcactggtc ggagctattg
     1681 gatcggcgtg cgttgcgcac ggcgacagtg atctgggcgc tctgtgcggc acaccgctcg
     1741 gcgacggccg ctttgggcca gtctgatccg cgcagcagcc cccggcgagt ctcgcgtgtg
     1801 agcgttctcc cgggcgctgc gctatgcgct acacgttcct cggcggggct acacgaggcg
     1861 ctggcggacg gcgtcggcga gcgactcggc gacgcagatc cggttggtcg tgtgcgggat
     1921 cgtgtcgcag gtgacgatgc cgcgggcgcc ggcgccgagc agcgcgtcgg ccagcgcatc
     1981 ggcgaacacc gcgtggatcg cgacgcacac cggcggggcg ctgcccgcgg cgcggagctg
     2041 gcgggtcgcc tcgatcatcg tccggccggt cgacacgatg tcgtcgatca cgaccggggt
     2101 gtggccgttc caggcgccgt cgggggcggt gatcgagacg tcgcgatcgc cgcgccgggt
     2161 cttctcgagg atcacgaacg gcgcgccgca gcgctcggcg accgccgaga cccactgcac
     2221 gctctcggcg tcggggccga tcagcaccgg gcgctggacc tcgtcggtga tccaccgcgc
     2281 gatcgccggc gcggcgcgcg cgatggtggt cgggatggtg tagacgccgg ccagcgagtc
     2341 gaggcggtgg aggtgcgggt cgacggtgac cagccagtcg accgtgcccg acaccaggcg
     2401 ggcgaagtag cgtgcggtga tgccctcgcc gggcttgaac cggctgtcct ggcgcatgta
     2461 ggcgaggtag ggcgcgacca ggccgacccg gctcgcgccg agctcgcgcg ccgtgccggc
     2521 gaggaacgcg acgcgcagga agctgtcggc ggagaggttg ccgacgatcg cgacgtcgcc
     2581 cctgcagtcg ccgtcgatcc ggacgtagat ctccccgtcg gggaactgcc ggatctccgg
     2641 cacgaggagg cgggccccga gtcgggtggc gagctcacgc gcgtgcgcgt cgccgccagg
     2701 tagggcgatc aggaccaaca cacggccatg ctagcagagc acggtggttc caggcggatc
     2761 gcgagcgacc attgccagcg gattcacccg cgcccgagcg cacaccgtgg gctgcagcgc
     2821 ttgcgcgatc gatgccgtcg ccgtccatgt cgccacccca ccgccacccg cgccgtcaca
     2881 gcgccttgag cggcgtcacg cgcagcagca gcgggatcac cttgccgctg gtcacgatcg
     2941 ccatgtgctc gaactgcgag tggatgtaca agagcttggc gccgcgctcg cgccgggtga
     3001 agtcgccgac ggcgggcggc atccgctcgc gggcgaccac gaacttgtag cgccgcgcga
     3061 agctcgacga ctcctcggcg gtgctggccg cgacgacgat cagcttgtgg tcgtcggcgc
     3121 gcttgccgct ggcgatgaac tcgtcgaacg ccgtctcacc ggcgtagaga gcgtcgagca
     3181 ggacgatctg gtcgaccagc cggtggtcga cccattgcat gacggtgcgg aacgccccgg
     3241 agtggccgat cacgatgacc gggccgtcgg gcaggtggat gttggcgcgg gtgacggcgc
     3301 ggcgcaggtc cttgagcgcc ggccactgga cctcgtcgtc gttgccggcg ggggcgtcgg
     3361 gcacgatgaa catcgcgttc tggcggctgg ccttgaactg ccgggcgagc tcgtgctcgc
     3421 gccacgcacc gtcggcgtcg gtgtagtagc cgtggacgta gatcaccgtg ccggcggtct
     3481 cacggtcgta gtcgggcggc acccagacgt gcacggcgcc gcgcgcggtc ttgatccgga
     3541 agtgcttgcc gcccagcacc gtatcctcct gcttgtcgag ccggccgtcg ccgtcgtcct
     3601 cgacgtgcga gcgcgccagg tgcgagcgcg ggctgaccga cagccgggcg cggtgccggt
     3661 ggccaggaga cgtggaaggc ggcgtggccg gtggccgggc gggcggacgt gcgggcgcgc
     3721 caggtgcgag cgcgggctga tcgacagccg ggcgcggtgg cggtgggccg gaggcgcggc
     3781 cggtggtcgg gcggggggcc gggcgggcgc g
//
LOCUS       DASVOR010002124         2239 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_257074,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002124 DASVOR010000000
VERSION     DASVOR010002124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2239
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257074"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VF516_39375"
     CDS             <1..365
                     /locus_tag="VF516_39375"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8113879.1"
                     /translation="APEPGVPAPAAAPSRAPLTHAEELIAAVWRELLGVDAIAVTDNF
                     LDLGGHSLLIMQAVAKLEARTGKRISPRTFIFQTLEQIAREYDAPRPEPPRPRPTSPT
                     RPPPSRLSRWLSALMPKP"
     gene            412..1353
                     /locus_tag="VF516_39380"
     CDS             412..1353
                     /locus_tag="VF516_39380"
                     /inference="COORDINATES: protein motif:HMM:NF025007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin-like domain-containing protein"
                     /protein_id="HEX8113880.1"
                     /translation="MPAPRIHLDPGSFEPWKIQAVTHALADHPLLQIDALVELGRRQQ
                     QRKLVRTHSNAATAGTSFADAPNLHPHRKDAATTLADIERAGAWMSLLNVQADPIYRR
                     LVDEVLDEVRPIVERRDPGMCYRAGWIFVSSPGTVTPFHMDHEHNFILQIRGKKRLYT
                     WDPFDREVVSERAQELFHDQHSRELVVWNEAWRPRARVFELEPGLGGYMPSTTPHMVE
                     NGDGPSITMSFTYYPDATRQRELLYRGNARLRRLGLAPRPVGASPGRDRVKRAVIAGY
                     TTAKNAVWRALGRSFRDTGLPYAPAQRNSPTAGLDAM"
     gene            1421..>2239
                     /locus_tag="VF516_39385"
     CDS             1421..>2239
                     /locus_tag="VF516_39385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113881.1"
                     /translation="MRADLPCAICGVLLVAGCGKSPAVPDGTGPPLIDAAPTDALVDV
                     PPHMPGTPGAGAHGLAYYKLADPRYPSNATSISTPKITTAASGSTIVVSVGRGDISKF
                     AAATVTDTAGNTPYQKQGPHPYTPAFPDSGTALYTFTNARGGPNFQVTTSTGRNANGQ
                     LDEITIAAVEVVEGTRIQDQQWNEVVQAQDPVPVTSNSVTTTGPATLVAFWWGDGFPG
                     TPQSATPGNGFSLIDSNAFETDSFVQCAVAVKNVSTAGTYHVTWTSTPIQGAQLW"
BASE COUNT          355 a          888 c          699 g          297 t
ORIGIN      
        1 ccgcaccgga gccgggcgtc ccggccccgg ccgccgcgcc gtcgcgcgcc ccgctgaccc
       61 acgccgagga gctgatcgcc gcggtgtggc gcgagctgct cggcgtcgac gcgatcgcgg
      121 tgaccgacaa cttcctcgac ctcggcggcc actcgctgct gatcatgcag gcggtcgcca
      181 agctcgaggc ccgcaccggc aagcggatca gcccgcggac cttcatcttt cagaccctgg
      241 agcagatcgc gcgcgagtac gacgcgccgc gtccggagcc gcccaggccg aggcccacct
      301 caccgacccg gccgcccccc tcgcggctgt cgcgctggct gtccgcgctg atgccgaagc
      361 catgaccgga gcctgccgat gagcacaccg cttcccgtat catggggcaa catgcccgcc
      421 cctcgcatcc acctcgatcc aggcagcttc gagccgtgga agatccaggc ggtgacccac
      481 gcgctggcgg atcacccgct gctccagatc gacgcgctgg tcgagctcgg ccgccgccag
      541 cagcagcgca agctggtccg tacccacagc aacgccgcga ccgccggcac cagcttcgcc
      601 gacgccccca acctgcaccc ccatcgcaag gacgcggcca ccacgctcgc cgacatcgag
      661 cgcgccggcg cctggatgtc gctgctcaac gtccaggccg atccgatcta ccgccgcctg
      721 gtcgacgagg tgctcgacga ggtcaggccg atcgtcgagc gccgcgatcc cggcatgtgc
      781 taccgcgccg gctggatctt cgtgtcgtcg cccggcaccg tgaccccgtt ccacatggat
      841 cacgagcaca acttcatcct ccagatccgc ggcaagaagc ggctctacac ctgggatccg
      901 ttcgatcgcg aggtcgtgtc cgagcgcgcc caggagctgt tccacgatca gcactcgcgc
      961 gagctcgtgg tgtggaacga ggcgtggcgg ccgcgcgccc gcgtgttcga gctcgagccc
     1021 gggctcggcg gctacatgcc gtcgaccacg ccccacatgg tcgagaacgg cgacggcccg
     1081 tcgatcacga tgagcttcac ctactacccc gacgcgaccc gccagcgcga gctgctctac
     1141 cgcggcaacg cccggctgcg ccggctcggc ctcgcgcccc gcccggtcgg cgcctccccc
     1201 ggccgcgacc gcgtcaagcg cgccgtgatc gccggctaca cgacggccaa gaacgcggtc
     1261 tggcgggcgc tcggtcgcag cttccgcgac accggcctcc cgtacgcccc ggcgcagcgc
     1321 aacagcccga ccgccgggct cgacgcgatg tgatcgattc cacgcgcgat cgcctggacg
     1381 cccggtcgat cgatgacaag atggcgatga ggagcggtcg atgagagcgg atctcccgtg
     1441 cgcgatctgc ggggtgttgc tggtggccgg ctgcggcaag tccccggcgg tgcccgatgg
     1501 caccggtccc ccgctgatcg acgccgcgcc gaccgacgcc ctggtcgacg ttcccccgca
     1561 catgcccggc acgccgggcg cgggcgcaca cggccttgcg tactacaagc tcgccgaccc
     1621 caggtacccc tcgaacgcca cgtcgatctc gacgccgaag atcaccaccg ctgcgtccgg
     1681 cagcacgatc gtcgtcagtg tcggtcgcgg cgatatcagc aagttcgccg ccgccaccgt
     1741 gaccgacacc gcgggcaata ctccgtatca gaagcagggc cctcacccgt atacacccgc
     1801 attccccgac tccggcaccg cgctctacac cttcacgaac gcgcgcggcg gccccaactt
     1861 ccaggtcacg accagcaccg gccggaacgc caacggccag ctcgacgaga tcacgatcgc
     1921 cgcggtcgag gtcgtcgagg gcacccggat ccaggaccag caatggaacg aggtggtcca
     1981 ggcccaggat ccggtcccgg tcaccagcaa cagcgtcacc accaccggcc cggcgacgct
     2041 cgtggcgttc tggtggggcg atggcttccc cggcactccg cagagcgcca cgccgggcaa
     2101 cggcttctcg ctgatcgaca gcaacgcgtt cgagaccgac tcgttcgtcc agtgcgcggt
     2161 cgcggtcaag aacgtctcga ccgccggcac ctaccacgtc acctggacct cgaccccgat
     2221 ccagggcgcc cagctctgg
//
LOCUS       DASVOR010002125         4791 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_139630,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002125 DASVOR010000000
VERSION     DASVOR010002125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4791
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139630"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..1837
                     /locus_tag="VF516_39390"
     CDS             242..1837
                     /locus_tag="VF516_39390"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8113882.1"
                     /translation="MAIVLFGVCAAGAALTTRPGVPTALAPVRVTSTTPYAPPPASST
                     SVPDVTACMGPAEVDDVGTIYRNSESEPFLAVNPTRANNMIIAHHVDRISVSGGAVGI
                     GVESTFDGGRTWASTLVPLTRCSGAAPDSPGDFERNTDPWIDFSRDGVAFLVGLPFNV
                     AEDFREAFTVARSLDGGQTWQGPFLIDLDPTAGTVNAYDEPKIAVDPVEPNTVYFSAV
                     NYPFKGPARKNQMVFSRSTDGGVTWPPLRVVGEFPPSQVNERFAIAAIPPSSRHPQGI
                     LVSAFGVAPSTPSPSDKAHVAVVRSFDRGDTWSAPATLGPIVTSALPLGEPFDAELGI
                     SFSIGETDIAVDPATGDVYAAWEDARFSGTSSAGVVVTRSRDGGATWSQPVSGNPGTP
                     PGVQASFEKLAVAADGTVGILFYDFRSDRPGDATLDTDAYLTLLTPTADGSLAFASEV
                     RLTPTSFDTRQFITRTTILAGFAGYFPGDYAGLRAAGNDFVAAFAVTNQNGTVPQFPI
                     EDSSLQVDSVNHQDIVFTRVRRR"
     gene            complement(1898..3232)
                     /locus_tag="VF516_39395"
     CDS             complement(1898..3232)
                     /locus_tag="VF516_39395"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8113883.1"
                     /translation="MATHRISRDRIEITRFQLKVVNGPDSPHRVESTGSELTIGSDPG
                     HPLRLTDPCVSRQHCMLRVSDEGVVCTDLGSTNGTLVTGRRITSAILLPGDTITVGET
                     TIAFDLLPDTVSEPLSVDDRFGRVLGRSVAMRRLFAIAERVAAADATILIDGETGTGK
                     SALAEAIHEHSPRKDKPFIVVDCGALPGTLLESELFGHERGAFTGALESRLGLFESAD
                     GGTILLDEIGELPLDLQPKLLRVLERRVIRKVGASRERPIDVRLIAATHRDLRCEVNR
                     KTFRSDLWYRLNTVRITMPPLRERRDDIAMLVWAIYQDLLGDRSAMPPTTIVRQLMRG
                     NWPGNVRELRSAVERSLLGAPVSDAAFDAGPTSFRDAKIRATADWERRYLADLLAAHD
                     GNVSRAARSARMNRSHLSELIHRHGLVAEPMAAEPEDRSDADGPRGEIPRSA"
     gene            3505..>4791
                     /locus_tag="VF516_39400"
     CDS             3505..>4791
                     /locus_tag="VF516_39400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113884.1"
                     /translation="MFSTTRSWLPFVALAAAGCTGDPPAATSTSTDPECQLDTKGEKT
                     PGYPFDVDKFTSDVLPIVSTSCSAGGCHAPPTGNAGFTVWASAPAGSCEFGKTFNNVV
                     RQLDLTTPGNSRLLAAVSGSTPGHPFVFPAGAPQLAALSGFAQSASQVLVTGGGGGVT
                     PPPGPSPFDYATFKSTIAPMFERCATAGCHAPPGQGGFAFKPAATADADLQANFTAVT
                     ALADFNNPATSLIYTQATTLHGSGKSLQVDAAQAQQLLAWITQAKGNAGTNTNPTCAP
                     VSKFNAGVFASEVMPILSGQLDLNVPGGVGRGAGCMSSVCHGTDRGPGKLSLLPTADV
                     ATQLQNFACFVDLASPLSSAVLACPFDLPSCPHSPHPGQQVFQGATDLNYQRVVSFVL
                     GANASVSPFDFAFFARRVNTIFDDINAVEQGAQGRSC"
BASE COUNT          707 a         1716 c         1625 g          743 t
ORIGIN      
        1 acgtctggct gcgcgcccgc caccggtgtc acgcgagctg gatgtgccac ggtgacccgc
       61 acgatctcgc ccaccgcgcg ccgacaccac agacccgcga cgagccgccg gtgaaggcca
      121 tgcaacctcg gcgcgtttcg gtcccgcgag ctcgttgacg cgcggcatca agcgtgcatc
      181 acgccggtgg tcgcgggtcg tgacacggga gttgcgtgtg aaggtggata cgtatctggc
      241 agtggcgatc gttctgttcg gggtctgtgc ggccggggcg gcgctgacga cccgccccgg
      301 tgtcccgacg gcgctcgcgc cggtccgggt cacgagcacg acgccgtacg cgccgccgcc
      361 ggcgagctcg acgagcgtgc cggacgtgac ggcgtgcatg ggccccgccg aggtcgacga
      421 cgtcgggacg atctaccgca acagcgagtc ggagccgttc cttgccgtca atccgacccg
      481 tgcgaacaac atgatcatcg cgcaccacgt cgaccggatc agcgtcagcg gcggcgcggt
      541 cggcatcggc gtggaatcca cgttcgacgg cggcaggacc tgggcttcca cgctcgtgcc
      601 gctcacgcgc tgctcggggg cagctccgga cagcccgggc gacttcgagc gcaacaccga
      661 cccctggatc gacttcagcc gcgatggcgt cgccttcctc gtcggcttgc cgttcaacgt
      721 ggccgaggac ttccgcgagg cgttcacggt cgcccgatcg ctcgacggcg gccagacgtg
      781 gcagggcccg ttcctgatcg acctcgatcc gaccgccggc accgtcaacg cgtatgacga
      841 gcccaagatc gccgtcgatc cggtcgagcc gaacaccgtc tatttctcgg ccgtcaacta
      901 tccgttcaag ggacccgcca ggaagaacca gatggtgttc tcgcgctcga ccgacggcgg
      961 ggtcacctgg ccgccgctcc gcgtggtcgg cgagttcccg cccagccagg tcaacgagcg
     1021 gttcgcgatc gccgccattc cgccgagctc ccggcacccg cagggcatcc tggtgtcggc
     1081 gttcggcgtc gcgccgagca cgccatcgcc ctcggacaag gcccacgtcg cggtcgtgcg
     1141 atcgttcgat cgcggcgaca cctggagcgc gccggcgacg ctcggtccga tcgtgacctc
     1201 cgcgctcccg ctcggcgagc cgttcgacgc cgagctcggc atctcgttca gcatcggcga
     1261 gaccgatatc gccgtcgatc cggcgaccgg cgacgtctac gccgcgtggg aggatgcccg
     1321 gttctccggc acgagctcgg ccggcgtcgt cgtgacccgg tcgcgcgatg gcggcgccac
     1381 gtggtcgcag cccgtgagcg gcaatccggg gacgccgccc ggcgtgcagg catcgttcga
     1441 gaagctcgcg gtcgcggcgg acggcacggt cggcatcctg ttctacgact tccgcagcga
     1501 tcgacccggt gacgcgacgc tggacaccga cgcgtacctg acgctgctca cgccgaccgc
     1561 cgacggctcg ctcgccttcg cgtccgaggt gcgcctgacc ccgacgtcgt tcgatacccg
     1621 ccagttcatc acccggacca ccatcctggc gggcttcgcg ggctacttcc ccggcgacta
     1681 cgccggcctg cgggccgcgg gcaacgactt cgtcgccgcg ttcgccgtca ccaaccagaa
     1741 cgggacggtg ccgcagttcc cgatcgagga ttcgtccctg caggtcgaca gcgtcaatca
     1801 ccaggacatc gtcttcaccc gcgtccgccg ccggtagcgg aaggaccgcg gcggtcgggc
     1861 tcggtggatc tggctgcgcg tcggggaggt gatcgcatca agcgctgcgc gggatttcac
     1921 cccgggggcc gtcggcgtcc gaccggtcct cgggctccgc ggccatgggt tcggcgacga
     1981 ggccgtggcg atggatcagc tcgctgaggt ggctgcggtt catccgcgcg ctgcgtgcgg
     2041 cgcgggacac gttgccgtcg tgcgcggcga gcaggtccgc caggtagcgg cgctcccagt
     2101 cggcggtggc ccggatcttg gcatcgcgga acgaggtcgg cccggcgtcg aacgcggcat
     2161 cggagaccgg cgcgccgagc agggagcgct cgaccgcgct gcgcagctcg cgcacgttgc
     2221 ccggccagtt cccgcgcatc agctggcgca cgatcgtcgt cggcggcatc gcggatcggt
     2281 cgccgagcag gtcctggtag atcgcccata ccagcatcgc gatgtcatcg cggcgctcgc
     2341 gcagcggcgg catcgtgatc cgcaccgtgt tgagccggta ccacaggtcg gagcggaacg
     2401 tcttgcgatt gacctcgcac cgcagatcgc ggtgggtcgc cgcgatcagg cggacgtcga
     2461 tcggccgctc gcggctcgcg ccgaccttgc ggatcacccg gcgctcgagc acgcgcaaga
     2521 gcttgggctg cagatcgagc ggcagctcgc cgatctcgtc gagcaggatc gtgccgccgt
     2581 cggccgattc gaacagcccg agccgcgact cgagcgcgcc ggtgaacgca ccgcgctcgt
     2641 ggccgaacag ctccgactcg agcaacgtgc cgggcagcgc gccgcagtcg accacgatga
     2701 acggcttgtc cttgcgcggc gagtgctcgt ggatcgcctc ggcgagcgcg ctcttgccgg
     2761 tgccggtctc gccgtcgatc aggatcgtgg cgtcggctgc ggcgacgcgc tcggcgatcg
     2821 cgaacagccg ccgcatcgcg acgctgcgcc cgaggacgcg accgaagcga tcgtccaccg
     2881 acagcggctc gctgacggtg tccggcagca ggtcgaacgc gatcgtcgtc tcgccgaccg
     2941 tgatcgtgtc gccgggcagc aggatcgcgc tcgtgatccg ccggcccgtg accagggtgc
     3001 cattggtgct gccgagatcg gtgcacacca cgccttcgtc gctgacgcgc agcatgcagt
     3061 gctgtcgcga cacgcacgga tcggtcagcc gcagcggatg accgggatcg ctgccgatcg
     3121 tgagctcgga tccggtcgat tcgacccgat ggggtgagtc gggtccattg accaccttca
     3181 gctgaaaccg ggtgatctcg atccgatcgc gggagatccg gtgtgtcgcc atcggggcga
     3241 tgccccaggc aatccaggtg cccgagctgt cgatgtgtgt ttccggtggc ttggtccgac
     3301 cagaatcgga gttgcgcaag cttcgggtac tacagagctt gcacaactct gattcagttc
     3361 atcgaaccct catggggagg cgctctccgc ccatgttggt gaacaccgac actcactcac
     3421 tccagatcgg agcgaaacga gcattctcga ggacttgacg aacggatcgt cccttgctga
     3481 ggcgctcctc catcaggagg gacgatgttt tccacgacga gatcttggct cccgttcgtc
     3541 gcgctcgcgg cggccggctg caccggtgac ccgcccgccg ccaccagtac gtcgaccgat
     3601 ccggaatgtc agctcgacac caagggcgag aagacgcccg gctatccgtt cgacgtcgac
     3661 aagttcacga gcgacgtgct gccgatcgta tcgacgagct gcagcgccgg cgggtgccac
     3721 gcgccgccca ccggcaacgc cggcttcacg gtgtgggcgt cggcgccggc ggggagctgc
     3781 gagttcggca agacgttcaa caacgtggtc cggcagctcg atctgaccac gccgggcaac
     3841 agccggctgc tcgcggcggt gtccggcagc acccccggtc atccgttcgt gttcccggcc
     3901 ggcgcgccgc agctcgcggc gctgagtggc ttcgctcagt ccgcctccca ggtactcgtc
     3961 accggcgggg gtggcggcgt gacgccgccg ccgggaccga gcccgttcga ctacgccacg
     4021 ttcaagtcga cgatcgcgcc gatgttcgag cgctgcgcga cggcgggctg ccacgccccc
     4081 cccggccaag gaggattcgc gttcaagcct gcggcgaccg cagacgccga cctccaggcg
     4141 aacttcaccg cggtcaccgc gctcgcggac ttcaacaacc cggcgacctc gctgatctac
     4201 acccaggcga ccaccctgca cgggagcggc aagtcgttgc aggtcgacgc ggcccaggcg
     4261 cagcagctgc tcgcatggat cacgcaggcg aagggcaacg ccgggaccaa cacgaacccg
     4321 acctgcgccc cggtgagcaa gttcaacgcc ggcgtgttcg cgagcgaggt gatgccgatc
     4381 ctgtccggcc agctcgatct gaacgtgccc ggcggtgtgg ggcgcggtgc cggctgcatg
     4441 tccagcgtgt gccatggcac cgatcggggc cccggcaagc tcagcctgct gccgaccgcc
     4501 gacgttgcga cccagctgca gaacttcgcg tgcttcgtcg acctcgcctc gccgctgagc
     4561 tccgcggtcc tcgcgtgccc gttcgacctc ccgagttgcc cccacagccc gcatcccggg
     4621 caacaggtgt tccagggcgc caccgacctc aactaccagc gggtcgtcag cttcgtgctc
     4681 ggcgcgaacg ccagtgtctc gccgttcgac ttcgcgttct tcgcgcgccg ggtcaacacg
     4741 atcttcgacg acatcaacgc ggtcgagcag ggcgcccagg gccgcagctg c
//
LOCUS       DASVOR010002126         1533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401888,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002126 DASVOR010000000
VERSION     DASVOR010002126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401888"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..>1533
                     /gene="ettA"
                     /locus_tag="VF516_39405"
     CDS             66..>1533
                     /gene="ettA"
                     /locus_tag="VF516_39405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEX8113885.1"
                     /translation="MTAQYTYVMKDLRKLVPPKREILKGIWLSFYHGAKIGVLGNNGA
                     GKSTLLRIMAGADKDFLGEALPAPGLKVGYLPQEPQLDPGKDVKGNVEEGVAETRALL
                     TRFEELSMKLGEDMSPEKMEKLLEEQGRLQDQIEAANAWELDHTVDMAMDALRCPPGD
                     ADVTKISGGERRRVALCRLLLSKPDMLLLDEPTNHLDAESVAWLERTLEEFKGTVVAI
                     THDRYFLDNVARWILELDRGEGHPFEGNYSGWLDYKAKRLRDEEKQSGARERMLAREL
                     EWVRSSPKARQAKSKARLQRYDEMVAEAQKEARDPALEIVVPPGPRLGNEVVIFDGVH
                     KSFGDRLLIENLSFKLPRGGLVGVIGPNGAGKTTLFRMIMGQEKPDRGTIKIGETVQL
                     GYVDQSRDALDADKSIYDEISEGAEQLQLGERAVNARAYVSSFNFKGQDQQKKVGILS
                     GGERNRVHLAKMIKRAGNVLLLDEPSNDLDVDTLRALED"
BASE COUNT          280 a          512 c          548 g          193 t
ORIGIN      
        1 ctcaccggcg gccccgtggc cgagggtagc cggcgccgcg gcgcgcgggt ataaccgccg
       61 cgaccatgac cgcccagtac acgtacgtga tgaaggatct ccgcaagctc gtgcctccga
      121 agcgcgagat cctcaaaggg atttggctgt cgttctatca cggcgccaag atcggcgtgc
      181 tcggcaacaa cggcgcgggc aagagcacgc tgctccggat catggccggg gccgacaagg
      241 acttcctcgg ggaggccttg ccggcgccgg ggctcaaggt cggctacctg ccgcaggagc
      301 cgcagctcga ccccggcaag gacgtcaagg gcaacgtcga ggagggcgtc gccgagaccc
      361 gggcgctgct gacccggttc gaggagctgt cgatgaagct cggcgaggac atgtcgcccg
      421 agaagatgga gaagctgctc gaggagcagg gccggctgca ggaccagatc gaggcggcca
      481 acgcctggga gctcgaccac accgtcgaca tggcgatgga cgcgctgcgc tgtccgccgg
      541 gcgacgccga cgtcaccaag atctcgggcg gcgagcgccg ccgggtcgcg ctgtgccggc
      601 tgctgctcag caagcccgac atgctgctgc tcgacgagcc gaccaaccac ctcgacgccg
      661 agagcgtggc gtggctggag cgcacgctcg aggagttcaa gggcacggtg gtcgcgatca
      721 cccacgaccg ctacttcctc gacaacgtcg ccaggtggat cctcgagctc gaccgcggcg
      781 agggccaccc gttcgagggc aactactcgg gctggctcga ctacaaggcc aagcggctgc
      841 gcgacgagga gaagcagtcg ggcgcgcgcg agcgcatgct ggcgcgcgag ctcgagtggg
      901 tgcggtcgtc gcccaaggcg cgccaggcca agagcaaggc ccggctgcag cgctacgacg
      961 agatggtcgc cgaggcccag aaggaggcgc gcgacccggc gctcgagatc gtggtcccgc
     1021 ccgggccgcg gctcggcaac gaggtcgtga tcttcgacgg cgtccacaag tcgttcggcg
     1081 accggctgct gatcgagaac ctgtcgttca agctgccgcg cggcggcctc gtcggtgtga
     1141 tcggccccaa cggcgccggc aagaccacgc tgttccgcat gatcatgggg caggagaagc
     1201 cggacagggg cacgatcaag atcggcgaga ccgtccagct cggctacgtc gaccagagcc
     1261 gcgacgcgct cgacgccgac aagtcgatct acgacgagat cagcgagggc gccgagcagc
     1321 tgcagctcgg cgagcgcgcg gtcaacgcgc gggcctacgt gtcgagcttc aacttcaagg
     1381 gtcaggacca gcagaagaag gtcggcatcc tgtcgggcgg cgagcgcaac cgcgtccacc
     1441 tcgccaagat gatcaagcgc gccggcaacg tgctgctgct cgacgagccg tccaacgacc
     1501 tcgacgtcga caccctgcgc gcgctggagg acg
//
LOCUS       DASVOR010002127        29524 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_227454,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002127 DASVOR010000000
VERSION     DASVOR010002127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 29524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29524
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227454"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VF516_39410"
     CDS             complement(<1..1151)
                     /locus_tag="VF516_39410"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEX8113886.1"
                     /translation="MNDVSRDVLRDVFGHAEPRPGQREAIDGVLGGRDVVVLLPTGAG
                     KSSCYQVPAVALARSGQGTTVVVSPLIALMNDQVGSLGARGVAAAAVHSQLDDAPRGD
                     AIRAFVRGELAVLYVSPERAVLDGFKRMLARTPIALVAIDEAHCVSQWGHDFRPEYLR
                     LHELREVVAAPVVALTATATPRVLHEIVRGLALRDPVIVRGDFRRPNLAFEVQHHRGD
                     ADRLAATIAVLDSAGLRGRTGPGRGIVYCSTRKKTETVAAELQAAGFAAMHYHAGRTG
                     LARDRAQRAFELGRARVLVATSAFGMGVDYPDVRAIVHFQAPGSLEAYYQEAGRASRD
                     GNPGRCVMLFGAADLVTQRRLSDGAGSGATSHRHEEALATLERYANAAR"
     gene            1487..2380
                     /locus_tag="VF516_39415"
     CDS             1487..2380
                     /locus_tag="VF516_39415"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8113887.1"
                     /translation="MCRTTDRIKKKAIRCNPSRRSPMRPASSADDLLAAPVGRYLAGR
                     SFVVWVQDRHRLGAFHFGRHEVVDEPALAALFALPLSSTLAPPYEVLYDLSAVEALDR
                     RAFECFEVFLARGVDQLAHRTRRLAAVRPNGLPGAAFAGLFHDLIAPRFDAKLCGDRR
                     EAYEWLGLGHGAQRDERDEIESLRDAFLEPVLVRRVREAIAADIPAATLDRIANALAI
                     SPRTLQRQLADHGTSLRDEVACARIRAARAMLLDRDEKIEYIASLVGFRSPASFTTRF
                     RAAVGESPSAFRALRTHRRAS"
     gene            2525..3655
                     /locus_tag="VF516_39420"
     CDS             2525..3655
                     /locus_tag="VF516_39420"
                     /inference="COORDINATES: protein motif:HMM:NF021916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEX8113888.1"
                     /translation="MQPRLHRLEAMLGEPLFDSILWGHEYDLSRLNPDLRARIESGHF
                     NRIVFYGMGCSSVVSDIVKGFFNVQKIPLHVDVINDYDMEWFIDSAIVKDDRTLTIIV
                     CYSGWSVEPCLFYDRMNELTKNRNLIVLSGGGKIADMCKADGSSLIQYKMRHADREYP
                     LYHVQQFFSIFLDLFHKLKITKSSYEEELRESVKYLKKTFDADTRKRAEAIAHKLQNS
                     RIVFLATAKWYVPLLKQVTMFFNEIAMVPSHRNLLHEFSHTEVAAYSNPKEKQSIVVL
                     RDSDEDDYTREKIATLEKLFGDKSVPQNKNIEFVTIDMNQKNFFQKFYYAHFFTVYVA
                     YYLGKLADVEGRDLISIAAKNPWWSARSIERFPKCVDIPGVL"
     gene            3673..4539
                     /locus_tag="VF516_39425"
     CDS             3673..4539
                     /locus_tag="VF516_39425"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8113889.1"
                     /translation="MATKRLTIITGGSRGLGHCLVQACLDIGDVLNVSRQPARRIESP
                     GSAGAHSVAGPHRLHNLPLDLGHPSEVEPALDVWLAAHPDHTVETLIHNAACLNLGWL
                     TDVAPGDLATAFAVNVFSPLAITARVFQAGRFAQRACRVAYVISSLARHDASLSFAGL
                     GVYSATKAALSRLALIQSREFELAAPHIKVLRIHPGIVDTEIQRELRQHPTLDPAFAA
                     KTAGLPPYREGDWIDRAPSDAMRTISAELAAEFVMWAMRSPDATSDEYDFYHSTPFHA
                     ARREREIQGRHA"
     gene            4577..6148
                     /locus_tag="VF516_39430"
     CDS             4577..6148
                     /locus_tag="VF516_39430"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8113890.1"
                     /translation="MTSRQLDTFWPNYIDGKYCRGGAGTIAVKDPSCNETLAEHALAD
                     AADIDRAVQSARRVHLSGALADLHPMERGQLVRKMGQYLANNIEEIKRILILEQGKPW
                     FEADVEVRQTVRLFDYFASVAESLDGRSVPTDSTRFAFTVHRPFGVSAQFIPCHYPVY
                     IPARSLAVALVTGNTCVLKTSELTPISATWFARAAEHAGLPAGALNIVCGRGADAGQA
                     LAAHPDIDHLVFGGYVESAAKVLTAAAPNLVPSVVEVGGTNPTIVFEDANMDAFESEA
                     RTGSYWNAGQFCGGMYRVIVHESRHDELVERSVALARSLRLVPGIETPRTQAPVYGPY
                     MGPLDSEKQVENVIGMVEGAVRAGAKCLTGGARVPGPGSFVQPTVLRDVLPEMAIAQK
                     EVWGPVMAVMKFRDIEEAFQIANSTPYTGLLCAIFTQDVGRMMRSAQSVRSAHVVMNA
                     SIVSGPEISFGGGFGRTGYGRIKGRDALLSYVQTKGVLLPIGDLLPETSRQTDAWLEE
                     RARVTGGAASSPFKD"
     gene            6166..7641
                     /locus_tag="VF516_39435"
     CDS             6166..7641
                     /locus_tag="VF516_39435"
                     /inference="COORDINATES: protein motif:HMM:NF037725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ligase PGM1-related protein"
                     /protein_id="HEX8113891.1"
                     /translation="MLEQILFGDTCTTPRTVVSIQSVSLGGLTAKYLRGLNYFEERNA
                     YFVDLLRNENCTCIVVLSDDVDRAFLHHTVNEGAKACNLSVGDLARRWKTVFVPTDGR
                     SSLSELLLGNRSALDSLRELVSSCENPILDFWTVTERELELAEALGLPHLGLRRELLA
                     VDSKSNSRAIFESLNIPHPLGYQDICNVDDARRALADLSARSPARSFLIKLNCEEAGN
                     GIARIQRSMIREPLADFVASLEVSKAIPVDVFVSEIAEQGAVVEEYIEASHTAFPSVK
                     MNVLPDGRVENLATHDQVLIGMAYAGSQFPADEAYRSDLMALGHKIATSLAARGVRGI
                     VSTDFIATRDVPTEPWSLWGLEVNARKGATTHPYFWSRLLTGATYDRATGRLVSPTGH
                     ITYQSSEYVQADGLKTVSPGELLKAMSDAGLAFDHKTHRGALVHMATSMRQFSKFGVT
                     IVGHGHQEITELQKRIVQLARQLTNPAKPGSTGGPAARHME"
     gene            7673..8914
                     /locus_tag="VF516_39440"
     CDS             7673..8914
                     /locus_tag="VF516_39440"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEX8113892.1"
                     /translation="MIGDLLHTGHLSVLEKARELGRVIVGVLTDQAVVSCNGLALSSF
                     AARKALVESVSGVSMVVPQETHSYRQNLERYRPAYLVHGDDWGNGSQAAVRQEAIKVL
                     SEWGGKLVEVPCTAELREDGPSSRSGAQRFRRLLAAKRLLRIIEVHDAISARIAENAT
                     ASGRAFDGFWSSSLIDSTVRGRPDIEVVDLKSRLDLLEEIMDCTSKPIIYDGDSGGNL
                     DQAFYLARTLSRTGVAALCVEDKAGRKRNSLYGRIAKSTQTSIKDFCERLRAVKHGVG
                     ASQLVVIARIESLVLHSDCDQAMERAQAYCEAGADAILIHSVADSPGEIFEFSRRFRA
                     VKPLVPLIAVPTAFDDVTASELEVNAFNVVIYANHLMRAASNAMIRAANDILAGDRGG
                     EIRQYSATPKDLLSFFPEPEF"
     gene            8937..10091
                     /gene="aepY"
                     /locus_tag="VF516_39445"
     CDS             8937..10091
                     /gene="aepY"
                     /locus_tag="VF516_39445"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: protein motif:HMM:TIGR03297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HEX8113893.1"
                     /translation="MKSTNQKQAIAESGAAPRGLLDVLVSCGVRFATGVPDSLLAPLC
                     DQVDGCRSIQHIIAASEGSALALGGGHYLATGQRPLIYMQNSGLPNAINPYLSMCHPS
                     AYAIPSIWVIGWRGEPGVPDEPQHRAIGAATEGLLALLNVPVFMLRSGDADELDRLEA
                     WMQDRAEGTVAVLVSARAVDADRRAPQRLEARPLKRREVLEHLVTGLDEEDTIFAGIG
                     HVGRELLAVRGGANEFAGDLLCVGGMGHASQFALGFALARPERRVWCLDGDGAFTMHM
                     GACSWLTRHPEQRFIHVLFDNGVHASVGGQPVCGQAVDFGRIAGALGYQHVERVSTLP
                     AAKAALDQARARRAPTFLWCMVDERSPSGLPRPRLELAARRDIFRSLHGD"
     gene            10084..11181
                     /locus_tag="VF516_39450"
     CDS             10084..11181
                     /locus_tag="VF516_39450"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEX8113894.1"
                     /translation="MTEVIMAPRAVERLPGILAGLGCRHCLVVTGASSVSAGAVEQCA
                     EYLPADHRVFSPAGTLPQLAEAMRCHRDLGDSFDGIIAIGGGRIIDTAKAIALRSQEL
                     VRFMDDPSHKLQVESRIPLVAVPTLAGSGSEVTPFAVAYRNGIKQSLSHPAMAPAAAV
                     VDPDLTATVSPRQRAISGLDAIAHGVEALLSKRAGATSDRHARRAIEIGYKALFKPAH
                     GPAEQLIDLCRASVSGGAAISTTRTTIPHAMSYYFTSVHGVPHGHAVALTMGRYLRAF
                     GIAAKADRQLRRWSESYDYVLHALGATGPHEVEGCWHDHLARLGISVSLSSIGIRAID
                     MSVLGQHVNRDRFENSPLNMSTAEARALLVE"
     gene            11202..12569
                     /locus_tag="VF516_39455"
     CDS             11202..12569
                     /locus_tag="VF516_39455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113895.1"
                     /translation="MDASRRTRLLGARILSEANDLKRTSDALADELELDRDHVARVIR
                     GEAGVQAALELIARMGEVYPIDAAELMVVEDDCVAGAKIMRAADSRATSRVFDRRDRH
                     GGRSPYYEYRDTAMSRLAPFRPEWIRELRVVGDADPKNPDVVFNKGHLLNQYTLFIGP
                     VNYYWEIDGVGHGREMTTGDSVFGTPWWPHTFTTREASKEAYILAVTFAGEVRRAQKE
                     LYALGPKARGWLLSVRQPRRAFIELVRHHMANDDLTLEELEQLVSAQAPGLELRRVFD
                     ENIELSWDEVVGLASCLGVEAGDLFVPRYKAEDEVLIKPHAPQQAVMLPSGSRRLYGI
                     VRMARTTKMPFMRGSMIHVLGRSASLDDGFCSSLYTWLYNYSTVPSRIAWTDGSETKQ
                     DVLAPGDSACLLPFVRYAFWAEEGEPTLCCIRVAGGVGLAAQRELSGLSDTERVFAET
                     GRWFE"
     gene            complement(12586..13836)
                     /locus_tag="VF516_39460"
     CDS             complement(12586..13836)
                     /locus_tag="VF516_39460"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8113896.1"
                     /translation="MDKPTFTAPKNRLLYVIEPGQQDPASHLAGSVAVRVYGRTLLDR
                     NIRLLLKQFDAIHVVLLVNEGSAGELQTALGEVPGSAGVTAQVVTSLDLCSVSDVDAV
                     LIQRGIGILENIASAAGEYYCASLSETEEHGDDRTTPIGIAWKAREAICGSENASCMV
                     RRQGATRGTPRRACVSTSKIRTHFLTDSDSSVASQLSSYLHAKASLRSMDGPVSRLLM
                     RRLSRRLSRPLGRWGVHPNVVTVVAAAVALFAALLFAIGDRSALLVGGVVWFVGGVLD
                     EVDGELARLQGKESEFGGWLDLTLDRIVDGLVLVGLAWPIAAGSSDSSLWALTALAIV
                     LVETSSYVGLLYDSWMKSALGRTVYFRIGRDTRNLAILLFSAFSLRVAAIVFVATISL
                     AEIARRLVVCLLWSRATARRDRVG"
     gene            complement(13878..15224)
                     /locus_tag="VF516_39465"
     CDS             complement(13878..15224)
                     /locus_tag="VF516_39465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8113897.1"
                     /translation="MAITRRLWTLSTPIIGINVLNVLMLAVDAALCGRLPDRDHTLAA
                     LGLSVQVVLLFMTAMLGLLVGTVALVSRAYGARDLARVNHLIAQSTILTAVVGVVVGA
                     IAAAYARVILAGFGASAVVAEIGADYLRPLMLGAPFAYLTLLYTGVLRGVGNTRIPFS
                     CAVIANIVNAVLNYGLVLGNLGMPALGVRGSAIGTVIAQLLNLVILVFALRRGAIANV
                     QLSLPRVHVDRRLAIELFRVGWPAAVDSLIMNVAFLSAIAMLARIDELTVAAHGLGMR
                     VRSLAFVPGLSIAQATGALVGQALGAGNVSQARVVMRAAMTLCLVIASGLALSIIVAA
                     RPLSHIFDVPSGTPLEALTVTWMRILGCTMVPAAIHISTYGLFHGAGATRTTLRINFW
                     VTMSFQIPLAYVLGFALELGELGVWLSFPIAIAVKASVNFIAYKREHWVVTGVRLA"
     gene            complement(15423..16004)
                     /locus_tag="VF516_39470"
     CDS             complement(15423..16004)
                     /locus_tag="VF516_39470"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqiC family protein"
                     /protein_id="HEX8113898.1"
                     /translation="MKSLVLALLLVACRSANSEYYTLVSPPRERPAPVTEAFQLDVLP
                     VDVPPEVDRPEIVVREGRGKLTPVETRSWISPLARELRHALSDDLTNALHVRDVAGAT
                     PVAGIPTYRIKLVVQRFDSSLGARASIEAISTIRDASSPAPAVVCSHRASEGAGPGYE
                     QLAEAHQRAVAAIAAQVAATIRGIQAGTAACRI"
     gene            complement(16010..17629)
                     /locus_tag="VF516_39475"
     CDS             complement(16010..17629)
                     /locus_tag="VF516_39475"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEX8113899.1"
                     /translation="MPVPVVRRRKPGWPSAVWLVPILAVVAAGGLAIRAYLSAGPTIE
                     ITFESAEGLEVGKSDVRYKNVPVGKISSVELTEDQRAILVKVELDRGAASLAAKDSRF
                     WVERPRIGIGGVSGVSTLLSGAYIGVDRGTSTDKTDRFVGLEKPPGVTHDQVGRRFRI
                     TAVDAGSLGVQSPVYLRRQQVGSISALALSPDGKHIDIEVFINSPYDRDVTDTAVFWN
                     ASGLDVSIDASGLHVDTQSLATVVAGGIAFGFREGDPPGHPAAEGAQFELFDDRAHAM
                     ARPDPTRLPLAMRFHQPLRGVGSGTQIDFEGVRIGQIDAINLGYDATTHGFFSDVDAT
                     VYPERLGAAYAALVDEGQRRGKTGPQMLQALVARGLRAQVRSASLITGQLFIALRWFP
                     GGRGTTPVAERDGVWVVPTERGGTDQIQDQVESIVAKIDKIPFDAIGGDARDAMQAAS
                     SLLGHVDREIVPTAQQLVGQAGAAMAALRDSLSALRDNVAAPDSAIQQTTRSTLEQLE
                     NAARSLRGLADYLQHHPESILRGRSSGTEPR"
     gene            complement(17655..18275)
                     /locus_tag="VF516_39480"
     CDS             complement(17655..18275)
                     /locus_tag="VF516_39480"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX8113900.1"
                     /translation="MIRGIDVRLATCHSCELVVRVCDFEDDAGAACPRCDASVRFRKR
                     NARTYVIALLVAALALYFPANALTIMYTEQFPERRADTILSGVKFLWERGSWGLAAIV
                     FTASILVPVLKLGALGFLVVSAMRHSAWRPRARTRLYRVLEVIGHWSMLDVFVVALLT
                     AVVQLGQFARVTPGPAVLPFAGVVILTMLASASFDARAIWDRGICE"
     gene            complement(18272..18877)
                     /locus_tag="VF516_39485"
     CDS             complement(18272..18877)
                     /locus_tag="VF516_39485"
                     /inference="COORDINATES: protein motif:HMM:NF016301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEX8113901.1"
                     /translation="MPAAELVICSYCDTVHRRAAPVGRETMRCVTCDSPIYRGNGDLG
                     AMLAVTVTAAIAFLVANALPLLTLTSGGHQTRATLWHAIAASYDSELPFVAIALTLTL
                     IVAPAIELGLLLWLLVPLCLWVRPLAFVSMMGLLRALRPWRMIEVFFLGVVVAVVKLA
                     ALATALPGWGMFGVAVMALALASLSSFDQGALWRRADEVMP"
     gene            complement(19076..20050)
                     /locus_tag="VF516_39490"
     CDS             complement(19076..20050)
                     /locus_tag="VF516_39490"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEX8113902.1"
                     /translation="MSRTWVDAFTADEIRELREVSNLRGALGIAGSWSMVAAAMVAVA
                     VAPLVLWPLVVPLALFVIGGRQLGMAILMHEAAHRTLFRSRRWNDRCANWLAAYPVYA
                     SVELYRPYHLQHHARTGTDEDPDIGLARGWPVSRASMRRKVVRDLTGVTGVKRLVAQT
                     RFLLALARGNPLGRSDALSFVGGGSAADARTQLGGTVLWQLGLIAVCAAAGHPLLYLL
                     WVGAWLTTYSLCMRIRSIAEHALTGDPGDPLTNTRTVLASPWERILFAPHRVNYHLEH
                     HLLMTVPCHNLPRLHDLLAQRGLLDGACLANGYREVLAAMVRAPAEPR"
     gene            20139..20366
                     /locus_tag="VF516_39495"
     CDS             20139..20366
                     /locus_tag="VF516_39495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113903.1"
                     /translation="MKEHRPGGGTLVGRATDGAAAAMPGKRTLTGDLELMQGGMATTE
                     GGVLFSGPDPKLAAETSIPPRRRRMPARSPS"
     gene            20854..21942
                     /locus_tag="VF516_39500"
     CDS             20854..21942
                     /locus_tag="VF516_39500"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HEX8113904.1"
                     /translation="MSKLFSEFHIKGVSLRNRIVVSPMCQYSAEDGVPDDWHLVHLGA
                     RAIGGAALVIAEATAVSPEGRITPGCTGLWNDRQAEAWAPIPRFVKRHGAVPGIQIAH
                     AGRKASANLPWEGDDHIPSSDPRSWDPLGPSPVAFGANLPRVPHAMTRDEILRVQRDF
                     VAAAKRALAVGFEWLELHFAHGYLAQSFFSPIANKRTDEYGGSFENRARFLIDTLSAV
                     RAIWPEKYPLTIRLGVNDFTKDSQPLEESIQLVKQFKHHGLDLIDVSLGFNTPDVSGV
                     PWGPAFMAPIAQRIKDEAAIATAVGWFITEPQQAEDMIAKGQADLVMLARKELDDPHF
                     PYHAAKALGVASPQNVLPPQYAHWLKRR"
     gene            22564..22701
                     /locus_tag="VF516_39505"
     CDS             22564..22701
                     /locus_tag="VF516_39505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113905.1"
                     /translation="MLLEAGRWCEVVWAFAVEVAAAYRAFTMASDLTVRLGDLSTSTI
                     P"
     gene            complement(23234..24175)
                     /locus_tag="VF516_39510"
     CDS             complement(23234..24175)
                     /locus_tag="VF516_39510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113906.1"
                     /translation="MSHGREYRPGKSSLSNWEHPATPTVAPGKTTLTQALDTDAAASK
                     TEPKSGATGKGRGNLAEMPVPAGGAKAEVAPAKHEEKDDKDDKGGKGKAEPKENAGAG
                     AGTPKHVTPTFVLTETEGARSNTAPATTNTDTPTFTGKVVKDGSVWRYQLETVEGKGR
                     IELVYYTVDHYPAPTPNDDSGALSNVNAGNWKAVVKDLKDHRTIVAGNYSSYRRTQLH
                     EHYHWETEWQGEVKKALRVAETEIAKLSSSAADATSAEAELAPKATRIFNDQMRAARA
                     AYNALPDTPGSAPYIAGAAGVDALVARIEAEVTTRKW"
     gene            complement(24188..24598)
                     /locus_tag="VF516_39515"
     CDS             complement(24188..24598)
                     /locus_tag="VF516_39515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113907.1"
                     /translation="MNGDLVCQLSVASEEPMALAVALAVPASAKSSLRVPRVLLPLGA
                     FVCVQIVDAHGATVYETNPPKFTPKLSPESPDAYVAVAPGSAHGERFVLDGASLAPGD
                     YEIRATYSNLYFQGPKENPIGEQRCTATLAYHKH"
     gene            complement(24851..24922)
                     /locus_tag="VF516_39520"
     tRNA            complement(24851..24922)
                     /locus_tag="VF516_39520"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24888..24890),aa:Gly,seq:gcc)
     gene            complement(25056..25616)
                     /locus_tag="VF516_39525"
     CDS             complement(25056..25616)
                     /locus_tag="VF516_39525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113908.1"
                     /translation="MRTASYAAAAAFASLLATPAVVHAQPSLVEPATPSSDPTAARSY
                     LEPGLDVGLATAGLYGALQLDGGHRLGDGPIWLHARLAHGGMGVIEETTMTSDFTDAR
                     VGLEARGCALDGIACLVSGLDAGYRHERLVTDYRNTRADLAAAIARLGLDLGGKHLRL
                     RASLETAVAQRGWDGLGLTTGIAYAW"
     gene            25852..27006
                     /locus_tag="VF516_39530"
     CDS             25852..27006
                     /locus_tag="VF516_39530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8113909.1"
                     /translation="MSRYIEPGKIAGALTLVARRGEVAWLSPLGQMDRERKKPMRADT
                     LFRIYSMTKPITSVALMMLHERGVFALGDPVHRFIPEWEGLRVYRYGKHPSWVTDPAA
                     RPMTMRDLLSHQSGLTYHIMERSPIDEAYRQLEVGAPGKTLREMIEQLAGLPLEFSPG
                     TRWGYSLATDVIGHLVEVLSGQRFDDYLRTQIFEPLGMVDTTFTVPADKLGRFAANYT
                     RLPGSTEATLMDDPMDSPFARPKAMLSGGGGLVSTAADYFRFCEMLRRGGELDGKRLL
                     GPRTVAYMTRNHLPDGRDLASLATAGSFSETRYEGIGFGLGFYVVVDPVRAQTPTSPG
                     EYGWGGMASTAFWINPADDLVVVFLTQLVPSTTFDFRGQLKAIVHAAIVD"
     gene            complement(27019..28416)
                     /locus_tag="VF516_39535"
     CDS             complement(27019..28416)
                     /locus_tag="VF516_39535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX8113910.1"
                     /translation="MKRTILAPALAAGFVLWGGCHSMAPAYQRPAAPVPASLPGGTAS
                     GGTATAAALPLRAFVREPRLQHILDQALAQSRDLRKAVLQIEAARAQYRIQRAQALPS
                     VDAIAQVNGGRSIVGPGNATATGALYSAQAGLAAWEIDVFGRLRSLSDAKLQSYLSTV
                     EAARAARISLIAETASAYLTLAADRSRLAIAQRTMEITRQTMDLTEQLVTGGAAGRGD
                     YWQAATVYQQARSDVALLTAAIGQDRNALDLLAGGHVDDALLPDALPAQLDWFSDVPV
                     GLASAVLLDRPDVLAAEHDLIAANANIGAARAQFFPSLTLTASGGLASAALTALFTGP
                     AAVWTLAPSLALPLFRGGANRANLELAEAQKRQLIASYELAIQTAFREVADALATRGT
                     IDEQLAAQAALVDAATKGYEVAEARYKAGVDTFLTTLVSQRALYTAQNSLIATQLAAL
                     GNRVTLYRVLGGGLQ"
     gene            complement(28413..>29524)
                     /locus_tag="VF516_39540"
     CDS             complement(28413..>29524)
                     /locus_tag="VF516_39540"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8113911.1"
                     /translation="PPAIQGLGNSQGFDFQLQAAGIDRAALAQARDQLLAAASKDPKL
                     IAVRRGDLPETPQLHIDIDQAKAVAHGEAPPDISRTLAAAWGGVYVNDFIDRGRVKRV
                     FVQGDAPFRSKPEDLDAWYVRGGSGAMTPFSAFATTRWTFGAESLSRYNGLASYDIQG
                     MGAPGVSSGEAMDEIARLARTVPGTTSSWSGLSYQEQLASGQTLALYSISILVIFLCL
                     AALYESWSVPFSVLLVIPLGIVGAVLAATLRGLENDVYFRVALLTTIGLSSKNAILIV
                     EFAEAAYHRGASLIDAAVTGARLRLRPILMTSLAFVAGVMPLALSTGAGANSRVSIGT
                     GIVGGTLTGTVIAVFFVPVFFVIVRRLVRGKRVPV"
BASE COUNT         5107 a         9697 c        10047 g         4673 t
ORIGIN      
        1 cagcgcgcgg cgttcgcgta gcgctccagc gtggcgagcg cctcctcgtg ccgatgcgat
       61 gtggcgcccg acccggcacc gtcggacagc ctgcgctgcg tcaccagatc cgccgcgccg
      121 aacagcatca cgcagcggcc cggattgcca tcgcgactcg cgcgcccggc ctcctggtaa
      181 taggcctcca ggctgcccgg cgcctggaag tggacgatcg cgcgtacgtc cgggtagtcc
      241 acgcccatcc cgaacgcgct ggtggcgacc agcacgcgcg cgcggccgag ctcgaacgcg
      301 cgctgcgcgc gatctcgtgc gaggccggtc cgcccggcat ggtagtgcat cgccgcaaac
      361 ccggccgcct ggagctcggc ggcgacggtc tcggtcttct tgcgcgtgct gcagtacacg
      421 atgccgcgcc ccggcccggt ccggccgcgc agacccgcac tgtcgagcac cgcgatcgtc
      481 gccgccagcc gatccgcgtc accgcggtga tgctggacct cgaacgccag gttcggccgc
      541 cggaagtcac cgcgcacgat caccggatcg cgcagcgcca ggccgcggac gatctcgtgc
      601 agcacccgcg gcgtcgccgt cgcggtcagc gcgacgaccg gcgcggcgac cacctcgcgc
      661 agctcgtgga gtcgcaggta ctcggggcgg aagtcgtggc cccactggct cacgcagtgc
      721 gcctcgtcga tcgcaacgag cgcgatcgga gtgcgtgcca gcatgcgctt gaagccatcg
      781 agcaccgcgc gctccggcga gacgtagagc accgcgagct cgccgcggac gaacgcgcgg
      841 atcgcgtcgc cgcggggggc gtcgtcgagc tggctgtgca ccgccgcagc cgccacgccg
      901 cgtgccccga ggctgccgac ctggtcgttc atcagcgcga tcagcggcga taccacgacc
      961 gtcgtgccct gcccgctccg cgcgagcgcg accgccggga cctggtagca cgacgacttg
     1021 ccggcgccgg tcggcagcag cacgacgacg tcgcggccgc ccagcacgcc gtcgatcgcc
     1081 tcgcgttgcc ccggccgggg ctcggcgtga ccaaacacgt cgcgcaggac gtcgcgcgag
     1141 acatcattca tccccgatcg atatcatccg ggtctgtcgc tgcgccgtcg gcggcggaca
     1201 ggtcgtcttc gccaattcct cgtcctcctc ggaacgattg acgtttccgc cccggcctgg
     1261 ccgcgctcaa gccggccgcg cgttcggacc gtggccgcgg tcgcggagct gacgccggag
     1321 cagcgcgcgc tcttgctcga caccaggcac tgagcgcatc cgttcgagcg gcgactccgc
     1381 cgcgccgggc ccgagagccc gggccgcgca ctccgcacgc accgccgcac atcgtgatcg
     1441 acggttggcg cgtcgcgaaa agaacttggc gtgttccaca gcaggcatgt gtcgtacaac
     1501 cgatcgcatt aagaaaaagg caattcgatg caatccttcg cggcggtcgc cgatgcggcc
     1561 cgcgagctcg gctgacgatc tgctggccgc tccggtcggc aggtatctcg ctggacggtc
     1621 attcgtggtg tgggtgcaag accggcaccg gctcggcgcg tttcacttcg gcaggcacga
     1681 agtcgtcgac gagcccgcgc tcgcagcgct gttcgcgctc ccgctgagct ccacgctggc
     1741 gccgccgtac gaggtgctct acgacctctc tgcggtcgag gcactggatc gcagggcctt
     1801 cgagtgcttc gaggtgttcc tcgcgcgcgg cgtcgaccag ctcgcgcatc gaacgcgccg
     1861 gctcgcagcc gtgcgaccga acggcctgcc gggcgccgcg ttcgcggggc tgtttcacga
     1921 cctgatcgcg ccgcggttcg acgccaagtt gtgcggcgat cggcgcgagg cgtacgagtg
     1981 gctgggtctc ggccatgggg cacagcgtga cgagcgcgac gagatcgaga gcctccgcga
     2041 cgcgttcctc gagcccgtgc tggtgcggcg ggtccgcgag gcgatcgcag ccgacatccc
     2101 agcggccacc ctcgaccgga ttgccaacgc gctcgcgatc agtccgcgaa cgcttcagcg
     2161 tcagctcgcc gaccacggaa cgagccttcg cgacgaggtc gcgtgcgccc gcatccgcgc
     2221 cgcgagggcg atgttgctcg atcgcgacga gaagatcgag tacatcgcat ccctcgtcgg
     2281 gttccgctcc ccagcatcgt tcaccacgag gtttcgtgcg gcggtcggtg agtcgccgag
     2341 cgcgtttcgc gcactccgca cgcaccgccg cgcttcgtga tcgacggttg gcgcgtcgcg
     2401 aaaagaactt ggcgtgttcc acagcaggca tgtgtcgtac aacccatcgc atcaaggaaa
     2461 aggcaattcg atgcaatcct tagaagatca ttcgaagtcc aacacttcat caggaacgcc
     2521 gatcatgcag ccgcgtctgc accgcctcga agcgatgctg ggagagccac tattcgacag
     2581 cattctgtgg ggtcatgaat acgatctgag tcggctgaat cccgaccttc gcgcgcgcat
     2641 cgagagcggc cacttcaacc ggatcgtgtt ctatggcatg gggtgttcgt cggtcgtctc
     2701 ggacatcgtc aagggtttct tcaacgtcca gaagatcccg ctccacgtcg atgtgatcaa
     2761 cgactacgac atggagtggt tcatcgacag cgccatcgtg aaggatgacc gcacgctcac
     2821 gatcatcgtg tgctacagcg gctggtccgt ggagccttgc ctgttctacg accggatgaa
     2881 cgaactgacc aagaaccgca acctgatcgt cctctcgggt ggcgggaaga tcgccgatat
     2941 gtgcaaggcg gatggtagct cgctcatcca gtacaagatg cggcacgccg atcgcgagta
     3001 tccgctctat cacgtgcaac agttcttctc gatcttcctc gatctgttcc acaagttgaa
     3061 gatcacgaag tcctcctatg aagaggaact tcgcgagtcg gtgaagtacc tcaagaagac
     3121 gttcgacgcg gatacccgaa agcgcgccga ggccatcgcg cacaagctcc agaacagccg
     3181 aatcgtgttt ctggcgacgg cgaagtggta cgtgccgctg ctcaagcaag tgaccatgtt
     3241 cttcaacgag atcgcgatgg tcccgtcgca ccgcaacctc ttgcacgagt tcagccacac
     3301 cgaggtcgcg gcgtactcga acccgaagga gaagcagtcg atcgtcgtgc tgcgggacag
     3361 cgacgaggat gactacacgc gcgagaagat cgcgacgctg gagaagctgt tcggggacaa
     3421 gagcgttccc cagaacaaga acatcgagtt cgtgacgatc gacatgaacc agaagaactt
     3481 cttccagaag ttctactacg ctcacttctt cacggtttac gtggcctact acctcggaaa
     3541 gctcgccgac gtcgagggtc gcgatctgat ctcgatcgcg gccaagaatc cgtggtggag
     3601 cgcccgctcg atcgagcgct tcccgaagtg cgtcgacatc ccgggagtcc tctagccgct
     3661 cgggtcggag ccgtggccac gaaacgactc acgatcatca ccggcgggag ccgggggttg
     3721 ggacactgcc tcgtgcaggc gtgtctcgac atcggtgacg tcctcaacgt atcccgacag
     3781 cccgcccgaa ggatcgaaag cccgggttct gcaggggcgc acagcgtcgc cggtccgcat
     3841 cggcttcaca acctgcccct cgacctcggc catccgagcg aggtcgaacc ggcgctcgac
     3901 gtctggctcg cagcgcatcc ggaccacacg gtcgagaccc tgatccacaa cgcggcgtgc
     3961 ttgaacctgg ggtggctcac cgacgtcgcg cctggtgatc tggcaaccgc gttcgcggtc
     4021 aacgtcttct cacccctcgc catcaccgcg cgcgtgttcc aggccgggcg gttcgcgcag
     4081 cgcgcgtgcc gcgtcgcata cgtgatctcg tcgttggcgc gccacgatgc cagcctgtcg
     4141 ttcgccgggc tcggcgtgta cagcgccacc aaagcggcgc tgagccggct cgcgctcatc
     4201 cagagccgag agttcgagct cgccgcgccg cacatcaagg tgctgcgcat ccaccccggc
     4261 atcgtcgata cggagatcca gcgcgagctg cgccagcatc cgacgctgga tccggcgttc
     4321 gccgcgaaga ccgccgggct gccaccgtac cgagagggag actggatcga tcgagcgccg
     4381 agcgacgcga tgcggacgat ctccgccgag ctcgccgccg agttcgtcat gtgggcgatg
     4441 cgctcgcccg acgcgacctc ggacgagtac gacttctacc actcgacgcc gttccacgcg
     4501 gcacgtcggg agcgcgagat ccaggggcgc catgcgtgag ccatctgagt ttcgaataac
     4561 tgattcggac agtgggatga caagcaggca actcgacacc ttctggccca actatatcga
     4621 tggcaagtac tgccgcggag gcgcgggcac catcgccgtc aaagatccga gctgcaacga
     4681 gacgttggcc gagcatgcgt tggcagatgc agcggatatc gacagggcgg tgcagtcggc
     4741 ccggcgcgtc catctttcgg gggcgctcgc cgacctccat cccatggaac gcggccaact
     4801 ggtccgaaaa atgggccagt acctggcaaa caacatcgag gaaataaagc gcatcttgat
     4861 actcgaacag ggcaagccct ggttcgaagc agatgttgaa gtcaggcaaa ccgttcgtct
     4921 gttcgactac ttcgcatcag tcgccgagtc cttggacggg cgatcggtgc ccaccgacag
     4981 cactcgcttc gccttcaccg ttcaccggcc cttcggtgtc tcggcacaat tcattccttg
     5041 tcattacccc gtatacatcc cggcgcggtc cctcgcggtc gccttggtaa cgggcaacac
     5101 atgcgtgttg aagacctcgg agctgacacc catttcagct acgtggtttg ctcgggcagc
     5161 agagcatgcc ggactaccgg ccggggctct caatattgtc tgcggtcgag gagcagatgc
     5221 cggtcaggcg ctcgcagccc acccggatat cgatcacctc gtgttcggcg gatatgtcga
     5281 gtctgctgcg aaggtcttga cggctgcggc tccgaatctc gtaccgagcg tggtggaggt
     5341 gggaggtacc aatccaacca tcgtttttga agacgcgaac atggatgcct tcgaatcgga
     5401 ggcgcgcact ggttcatact ggaacgccgg ccagttctgc gggggcatgt accgggtgat
     5461 cgtccacgag tcacggcacg atgagctcgt cgagaggtcg gtcgcactgg ctcggtcgct
     5521 caggctggta ccgggtatcg agaccccccg cacgcaagct ccggtctatg ggccgtacat
     5581 ggggccgctg gacagcgaga agcaggtcga gaacgtcatc ggcatggtcg aaggtgcggt
     5641 gcgggcaggc gcgaagtgcc tgaccggcgg cgcgagggtc ccgggtccgg gctcgttcgt
     5701 gcagcccacg gtgttgcgcg acgtgctccc cgagatggcc atcgctcaga aagaggtgtg
     5761 ggggccggtc atggcggtca tgaagttcag ggacatcgaa gaggcgtttc agatcgccaa
     5821 cagtacacct tacactggcc tgctctgcgc gatcttcact caagacgtcg gcaggatgat
     5881 gcgctctgcc cagtcggtgc ggtctgcgca cgtagtgatg aatgcgtcca ttgtctcggg
     5941 gccagaaata tcgttcggcg gtggcttcgg aaggacggga tacggtcgca tcaagggtcg
     6001 cgacgccctt ctgagctacg ttcagacaaa gggtgtgctg ctaccgatag gagatctctt
     6061 gccggagacg tctcgacaaa cagatgcatg gctcgaggag cgagcgcggg tcaccggcgg
     6121 cgcggcatcg agcccgttca aagactgagc cactggtaaa gagacatgct cgaacagatc
     6181 ctctttggcg atacatgcac gactccacgg accgtggtgt cgatccagag cgtgagcctc
     6241 ggcgggctga cggcgaaata tctgcgcggg ctgaactact tcgaagagcg aaacgcatac
     6301 tttgtcgatc tgctgcgcaa tgagaattgt acgtgcatcg tcgtcctctc cgacgatgtc
     6361 gacagagcct ttcttcacca cacagtgaat gagggcgcca aggcctgcaa cctgagcgtc
     6421 ggcgatctcg cgcgtcgatg gaagacggtc ttcgtcccga cggacggtcg gtcgagtctc
     6481 tcggagcttc tgctcggcaa ccggagcgct ctcgattcac tgcgcgagct cgtcagcagc
     6541 tgcgagaacc ccatcctcga cttctggacg gtcaccgagc gcgagctcga gctcgccgag
     6601 gcgcttggcc tgccccatct cggcctgcgc agggaactgc tggccgtcga ttccaaatcg
     6661 aacagtcgag cgatcttcga atcattgaac ataccgcatc cgctggggta ccaggacatc
     6721 tgtaacgtgg acgatgcgcg aagagcgctg gctgatctgt cggcccgctc tccagctaga
     6781 tcatttctga tcaagctgaa ttgcgaagag gcgggcaacg gaatcgcacg aattcagcga
     6841 tccatgatca gagagccact cgccgacttc gtagcaagtc tggaagtctc gaaggcgatt
     6901 cccgtcgacg tcttcgtctc ggagatcgcc gagcaaggcg cagtcgtcga ggagtacatc
     6961 gaggcgtcgc acacggcatt ccccagcgtg aagatgaatg tcttgccaga tgggcgagtc
     7021 gagaatctgg cgactcatga tcaagtactc atcggcatgg cctacgccgg gtcccagttt
     7081 cccgccgatg aagcgtatcg cagcgacttg atggcgctcg gtcacaagat cgccaccagc
     7141 ctggcggcca ggggggtccg cggcatcgtc tccacggact tcatcgcgac ccgcgacgtt
     7201 ccaacggaac catggagtct gtggggtctc gaggtcaatg cgagaaaggg tgccaccacg
     7261 cacccgtatt tctggtccag actcctgacc ggcgccacct atgaccgagc aaccggtcgg
     7321 cttgtttcgc ccacagggca catcacgtat caatcatcag agtacgtgca agcagatggt
     7381 ttgaaaaccg tcagccccgg agagctcctg aaggccatgt ccgacgcagg tctggcattc
     7441 gatcacaaga ctcatcgagg tgcccttgtc cacatggcca ccagcatgcg gcaattttcc
     7501 aaattcggcg tcaccatcgt cggccatggc catcaggaga tcactgaatt gcagaaacgg
     7561 attgtccaat tggctcgaca gctaacgaat ccagcgaaac cgggctccac cgggggacct
     7621 gccgctcgtc acatggagta atcctatgtc aagtcagaca gtgtatgtag ggatgatcgg
     7681 agacctgctc cacacgggtc acctcagcgt gctggaaaag gctcgcgagc tggggagggt
     7741 gatcgtcgga gttctgaccg accaggccgt ggtgagctgc aacgggttgg cgctctcctc
     7801 gtttgctgca cgcaaggcgc tcgtcgagag cgtgtccggc gtgtccatgg tggtgccgca
     7861 agaaacccac agctaccgac agaacctcga gcgctatcgg cccgcgtacc tcgttcacgg
     7921 cgatgattgg ggcaacggct cgcaagccgc agtgcggcag gaggcgatca aggtcttgtc
     7981 ggagtggggt ggcaagctgg tcgaggtgcc gtgcaccgct gaactgcgcg aggacggccc
     8041 gagctcgcgg tccggtgcgc aacggttccg gcggcttctg gcggccaagc gcttgctgcg
     8101 gatcatcgag gttcacgacg cgatctcggc tcgcatcgca gagaatgcga ccgcctcggg
     8161 tcgtgcgttc gacggtttct ggtcgagcag cctgatcgac tcgaccgtac gtgggcggcc
     8221 cgacatcgag gtcgtcgacc tgaagtcgcg attggatctg ctggaagaga tcatggactg
     8281 tacgtcgaag ccgatcatct acgacggaga tagcggtggg aatctagatc aggcgttcta
     8341 tctggcgcgc accctgtcga ggacgggcgt ggcagccttg tgcgtggaag acaaggcagg
     8401 ccgaaaacgg aactcgctct atggccgtat cgccaagagc acgcagacct ccatcaagga
     8461 cttctgcgag aggcttcgcg cggtcaagca cggcgtgggg gcttcgcagc tcgtcgtgat
     8521 cgccaggatc gagagcctcg tgttgcactc ggattgcgac caggcgatgg agcgagctca
     8581 ggcatattgt gaagcaggcg ccgacgcaat tctcattcac agtgtggctg actcgccggg
     8641 cgagattttc gaattcagcc gccgcttccg cgccgtgaag ccgctggtgc ccctgatcgc
     8701 agttcccaca gcgttcgacg atgtgaccgc gtcggagctg gaagtgaacg cgttcaacgt
     8761 cgtcatctat gcaaaccatc tcatgcgcgc cgccagcaac gcgatgatca gggcggcaaa
     8821 cgacatcctg gccggcgacc gcggtgggga gattcgtcag tacagcgcca ctccaaagga
     8881 tctgctgtcc ttcttcccgg agcctgaatt ctagagcgag ccggccgaac caacccatga
     8941 agtctaccaa tcagaaacag gcaatcgcag agtcgggggc ggcaccccgg ggcctcctcg
     9001 acgtgctggt gagctgcggg gtgcgatttg cgaccggcgt gcccgacagt ctgctggccc
     9061 cactgtgcga tcaggtggac ggttgcagat cgatccagca catcatcgcg gccagcgagg
     9121 gctcggccct ggcgctggga ggggggcact acctggcgac gggccagcgc cccctgatct
     9181 acatgcagaa ctccggtctg cccaacgcca tcaacccgta cctgtcgatg tgccaccctt
     9241 ccgcgtacgc catcccttcg atctgggtga tcggctggcg cggagagccc ggtgttccgg
     9301 acgaacccca gcatcgagcc atcggcgcgg cgaccgaggg cttgctcgct ctattgaacg
     9361 tgcccgtgtt catgttgcgg agcggcgacg cagacgagct cgatcgcctg gaagcgtgga
     9421 tgcaggacag ggcggaagga accgtggctg ttctcgtgtc tgcccgcgcg gtggatgcgg
     9481 atcgccgagc gccccagcgc ctcgaggccc gaccgctcaa gcgtcgagag gtgctcgagc
     9541 acctggtgac ggggctcgac gaggaggaca cgatcttcgc gggtatcggc cacgtgggtc
     9601 gagagttgct ggcggtgcga ggcggtgcga acgagttcgc cggggaccta ctttgcgttg
     9661 gaggaatggg tcacgccagc cagttcgcgc tgggcttcgc gctggcccgc ccggaacgtc
     9721 gtgtgtggtg cctcgacgga gacggagctt tcacgatgca catgggggcg tgtagctggc
     9781 tcacacgcca ccccgagcag cggttcatcc acgttctctt cgacaatggg gtccacgcat
     9841 cggtcggagg ccagccggtc tgtggacaag cggtcgactt tggccgcatc gccggagcgc
     9901 tcgggtacca gcacgtcgag agagtctcga cgctgccggc ggcgaaggca gcgctcgacc
     9961 aggcgcgagc gcgtcgcgca ccgaccttcc tctggtgcat ggtcgacgag cggagcccgt
    10021 cgggcttgcc acggccacgc ctcgagctcg cggctcgacg tgacatcttc cggagcctcc
    10081 acggtgactg aggtgatcat ggcgcccagg gcagtcgagc ggctgcccgg gattctcgcg
    10141 ggcctcgggt gccggcactg cctggtggtc acgggcgcgt cgtccgtcag cgcgggcgcg
    10201 gtggaacagt gcgccgagta cctgccggcc gaccatcgcg tgttttctcc agccggcacg
    10261 ctgccgcaac tggccgaggc gatgcgatgc catcgcgatc tcggcgattc gttcgacggc
    10321 atcatcgcga tcggcggagg acgcatcatc gacacggcga aggcgatcgc gcttcgcagc
    10381 caggagctgg tgcggttcat ggatgatccg tcccacaagc tccaggtcga gagccgcatt
    10441 ccacttgtcg cagtaccgac acttgccggt tcgggaagcg aagtcacgcc gttcgccgtt
    10501 gcctatcgaa acggaatcaa acagtccctc agtcatccgg ccatggcgcc cgccgccgcc
    10561 gtcgtcgatc cagacttgac cgccaccgtt tcacctcggc agcgcgccat ctctggcctc
    10621 gatgcgatcg cgcacggcgt cgaagccctg ctctcgaagc gtgccggcgc gaccagcgac
    10681 cgtcatgcgc gaagagccat cgagattggc tataaagctc tgttcaagcc agcacacggt
    10741 ccggcggaac agctgatcga tctctgtcgt gcatccgtat cgggtggcgc ggcgatctcg
    10801 acgacaagaa ccacgattcc tcatgcgatg tcgtactatt tcacctcggt ccacggtgtt
    10861 ccgcacggcc atgcggtggc gctgaccatg gggcgctatt tgcgggcatt tggtatcgcc
    10921 gcgaaagcag acaggcagtt acggaggtgg tctgaaagct acgactatgt tcttcatgcg
    10981 ctcggcgcga ccgggcctca cgaagtggaa ggctgctggc acgaccacct cgcgcgcctg
    11041 ggtatcagcg tgtcgctgtc gtccatcggg atacgagcga tcgacatgag cgtgctcgga
    11101 cagcacgtga atcgtgatcg tttcgagaac tcgccactga acatgtcaac cgccgaggcc
    11161 agagccttgc tggtcgagta gccgtttgac gaggtacttc gatggacgct agccgccgga
    11221 cacgtctgct cggggcgcga atcctgagcg aggcaaacga tctcaagcgc accagcgatg
    11281 cgcttgccga cgagctcgag ctcgatcgcg accacgtcgc tcgggtgatc cgcggtgagg
    11341 caggggtgca ggccgcgctc gagctcatcg cgcgaatggg tgaggtctac ccgatcgacg
    11401 ccgccgagct gatggtcgtc gaagatgatt gcgtcgccgg tgccaagatc atgcgcgccg
    11461 ccgattcgcg agccacgtcc cgggtcttcg accggcgcga tcgtcatggg ggccgcagcc
    11521 cgtactacga gtaccgcgac accgcgatgt cgcgcctggc gccgtttcga cccgagtgga
    11581 ttcgcgagtt gagggtggtg ggtgacgccg acccgaagaa ccccgacgtg gtcttcaaca
    11641 agggccacct gctcaatcag tacacgctct tcatcggtcc ggtgaactac tactgggaga
    11701 tcgacggggt cgggcatggg cgcgagatga cgaccggaga cagcgtcttc gggacaccct
    11761 ggtggcccca caccttcacg acgcgcgagg catcgaagga ggcctacatt ctcgcggtga
    11821 cctttgccgg cgaggtacgc cgggcccaaa aggagctgta tgccttgggc cccaaggcga
    11881 gaggttggtt gctttcggtc agacaaccgc ggcgcgcctt catcgagctg gtgcgtcatc
    11941 acatggccaa tgacgacctc acgctggagg aactcgaaca gctggtcagc gcgcaggccc
    12001 caggtctgga gctgcggcgc gtgttcgacg agaacatcga gctcagctgg gacgaggtgg
    12061 tggggctcgc cagctgtctg ggggtcgaag ccggcgacct gttcgtgccc cgctacaagg
    12121 ccgaggacga ggtgctgatc aagccccacg cgccacaaca ggccgtgatg ctcccttccg
    12181 gttcacggcg cctgtacggc atcgttcgca tggcgaggac gacgaagatg cctttcatgc
    12241 gcgggtcgat gatccacgtc ctgggcagat cagcgtcgct ggacgatggc ttctgtagct
    12301 cgctctacac gtggctgtac aactacagca ccgtcccttc gcggatcgcc tggaccgacg
    12361 ggtctgaaac gaaacaagac gtgttggccc ccggtgactc cgcatgcctc ctgccgttcg
    12421 tgcggtacgc gttctgggcc gaggagggcg agccgaccct gtgctgcatt cgcgtcgcgg
    12481 gaggcgtggg gctcgcggcg cagcgcgagc tgtcgggact ctcggacacc gaacgtgtgt
    12541 tcgcggagac cggccgctgg ttcgagtagt cgtcgaccct gccgatcaac caacgcgatc
    12601 acgtctcgca gtcgcgcggc tccatagcaa gcaaaccact aacctgcgcg cgatctcggc
    12661 caacgagatg gtggccacga agacgatggc cgcgacgcga agcgaaaacg ccgagaacaa
    12721 caagatcgcc aagttgcgag tgtctcggcc aatgcgaaag tagacggtgc ggcccagggc
    12781 actcttcatc cagctgtcat agagcaaccc gacatagctg ctcgtttcca ccagaacgat
    12841 cgcgagcgca gtcagcgccc acagagaaga atctgaagat cctgctgcga tcggccaggc
    12901 cagaccgacg agcacgagac cgtccacgat acggtcgagg gtcaagtcca gccatccgcc
    12961 aaattcactc tccttcccct gcaggcgcgc gagctctccg tcgacctcat ccagtactcc
    13021 gccgacgaac cacaccacgc cgcccacgag gagcgcgctt cgatcgccga ttgcaaaaag
    13081 caatgccgcg aacagtgcga ctgcggcggc taccacggtg acgacattgg ggtgaacgcc
    13141 ccagcggccg agaggccgtg agagacggcg cgacagccgc cgcatcaaca acctcgaaac
    13201 tggaccgtcc atcgacctca gtgatgcctt ggcgtgaagg tacgacgaaa gctggctcgc
    13261 aaccgagctg tccgagtcgg tgagaaaatg agtgcggatc tttgacgtcg agacacaggc
    13321 ccgacgaggg gtgccccgag tcgctccctg tcgccggacc atgcagctgg cgttctcgga
    13381 gccacaaatt gcctcacgcg ccttccaggc gatccctatg ggtgttgtcc tgtcgtcacc
    13441 atgctcctct gtctcggaga gtgatgcgca gtagtactca ccagccgccg aggcgatgtt
    13501 ctccagaatc ccaatgccac gctgaattaa tactgcatcg acatcagaga ccgagcagag
    13561 atccaacgac gttacgactt gtgccgtcac gcctgcgctg cctggaacct cgccgagagc
    13621 ggtctgcagc tcaccggcgg agccctcgtt gactaacaag acaacatgaa tagcgtcgaa
    13681 ctgcttcaga agcagcctga tgtttcgatc gagaagggtt cggccgtaga cccggacggc
    13741 gaccgatcct gccaggtgag aggccgggtc ttgctgtcct ggctcgatga catagagcag
    13801 acggttcttc ggagctgtga acgttggctt gtccaagaca cggtgcacac ggtgctccat
    13861 cgttgcggtg gggtgtatta cgcgagacgc acgccagtga cgacccagtg ctcgcgcttg
    13921 tacgcgatga agttgacact cgccttcacc gcgatcgcga tcggaaacga cagccagaca
    13981 ccgagctcgc cgagctcgag cgcgaagccg aggacgtagg cgagcgggat ctggaacgac
    14041 atcgtcaccc agaagttgat gcggagcgtg gtccgcgtgg cgccggcgcc gtgaaacagg
    14101 ccatacgtgc tgatgtggat cgcggccggc accatcgtgc atccgaggat gcgcatccac
    14161 gtgacggtga gcgcttcgag cggggtaccc gacggcacgt cgaagatgtg cgagagcggg
    14221 cgcgcggcga cgatgatcga cagggcgagg ccgctcgcga tcacgaggca gagcgtcatc
    14281 gccgcacgca tgaccacgcg cgcctggctg acgttgcccg cgccgagcgc ctggccgacg
    14341 agggcaccgg tcgcctgcgc gatcgacagt ccgggcacga acgccagcga ccgcacccgc
    14401 atgccgaggc cgtgggcggc gacggtgagc tcgtcgatgc gcgcgagcat ggcgatcgcg
    14461 ctcaggaacg ctacgttcat gatcaagctg tccacggcgg cgggccagcc gacccggaac
    14521 agctcgatcg cgagccggcg atcgacgtgc acccgcggca gcgacagctg cacgttcgcg
    14581 atcgcgccgc gacggagcgc gaacacgagg atgacgaggt tgaggagctg ggcgatcacc
    14641 gtcccgatgg ccgagccgcg cacgccgagc gccggcatgc cgaggttccc caggacgagc
    14701 ccgtagttga gcactgcgtt gacgatgttg gcgatcaccg cgcaggagaa cgggatgcgc
    14761 gtattgccga cgccgcgcag cacgcctgtg tagagcaacg tgagatacgc gaacggcgcg
    14821 ccgagcatga gcgggcgcag gtagtcggcg ccgatctcgg cgacgacggc agaggcgccg
    14881 aacccggcca ggatcacgcg cgcgtacgcc gccgcgatgg cgccgaccac gacgccgacc
    14941 acggccgtga gtatcgtcga ctgcgcgatg aggtggttga cgcgagcgag atcgcgggcg
    15001 ccgtacgcgc gcgagaccaa ggcgaccgtg ccgacgagca ggccgagcat cgcggtcatg
    15061 aacaacaaca ccacctgcac cgagagcccg agcgctgcga gcgtgtgatc gcggtccggc
    15121 agccggccgc acagcgccgc gtcgaccgcg agcatcagga cgttcagaac attgatcccg
    15181 atgatcggtg tcgacagcgt ccacaaccga cgcgtgatcg ccaaccctga cgtctgttgc
    15241 tgctcgggca attgcgcgac gctagcagga gggtccgcgt tgccgagcgc gtgtcagcac
    15301 tttggcgttc gggaacaaca acttgtcatg tcgggtgtgg tgatcggtcc tgcccagacg
    15361 atgctcgcgg agctgcgcga cgccgatccg ggggcggcgg gctgccgcgc tacgtcgagc
    15421 ggtcagatgc gacaggcagc ggtgccggct tgtatgccgc ggatcgtcgc tgcgacctgc
    15481 gcggcgattg cagccacagc gcgttgatgc gcttcggcga gctgctcgta accagggccc
    15541 gctccctcgc tcgcgcggtg cgagcacacc acggcgggcg ccgggctgct cgcatcccgg
    15601 atcgtggaga tcgcctcgat cgacgcacgc gcgccgaggc tcgagtcgaa gcgctgcacg
    15661 acgagcttga tcctgtaggt cgggatacct gcgaccggag tcgcgccggc gacatcgcgc
    15721 acgtgaagcg cgttggtgag gtcgtcggac aatgcgtggc gtagctcgcg agccagcggc
    15781 gagatccacg accgggtttc gaccggcgtc aacttgccgc gaccctcgcg cacgacgatc
    15841 tcggggcggt cgacctcggg cggcacatcg acgggcagga cgtcgagctg gaacgcctcg
    15901 gtcaccggtg cgggccgctc cctcggcggt gacaccagcg tgtagtactc gctgttcgcc
    15961 gagcggcatg cgaccaggag caacgccagg acgagcgact tcatggacct caccggggtt
    16021 ccgtgcccga cgagcggcca cggaggatcg attcggggtg atgctgcagg taatcggcga
    16081 gcccgcgcag ggagcgcgcg gcgttctcga gctgctcgag cgtggatcgc gtcgtctgct
    16141 ggatcgccga atcgggagcc gcgacattgt cgcgcagcgc gctcaggctg tcgcgcagcg
    16201 cggccatcgc ggcgccggcc tgaccgacga gctgctgcgc cgtcggcacg atttcgcgat
    16261 cgacgtgccc gagcagcgag gacgccgcct gcatcgcgtc gcgggcatcg ccgccgatcg
    16321 catcgaacgg gatcttgtcg atcttcgcga cgatactctc gacctgatcc tggatctggt
    16381 ccgtgccgcc acgctcggtg ggcacgaccc acaccccgtc gcgctctgcg acgggagtcg
    16441 tgccgcgtcc gcctggaaac cagcggagcg cgatgaacag ctggccggtg atgaggctcg
    16501 cagagcggac ctgcgcccgc aaaccgcgcg cgacgagcgc ttgcagcatc tgcggtccgg
    16561 tcttgccgcg acgctggccc tcgtcgacca gcgccgcgta tgccgcgccc aatcgctcgg
    16621 ggtagaccgt cgcgtcgaca tcggagaaga acccatgggt ggtcgcgtcg tagccgaggt
    16681 tgatggcatc gatctgaccg atgcgaacgc cttcgaagtc gatctgcgtg cccgagccga
    16741 cgccgcgcag cggctggtga aacctcatcg cgagcgggag ccgcgtcgga tccggccgcg
    16801 ccatggcgtg cgcacgatca tcgaacaact cgaactgcgc gccctcggcg gccgggtggc
    16861 ccggcgggtc gccctcgcga aacccgaacg cgatgccacc ggcgaccacg gttgccagtg
    16921 attgcgtatc gacgtgcaga cccgaggcat cgatcgagac gtcgagtccg ctggcattcc
    16981 agaacaccgc cgtatcggtg acgtcgcgat cgtaggggct gttgatgaag acctcgatat
    17041 cgatgtgctt gccgtcagga ctcagcgcga gcgccgagat cgacccgacc tgttggcgcc
    17101 gcagatagac cggtgattgc acaccgagcg agccggcgtc gaccgccgtg atccggaatc
    17161 gccgacccac ctgatcgtgg gtcacgccgg gcggcttctc gaggccgacg aaccgatcgg
    17221 tcttgtcggt cgacgtgccg cggtcgaccc cgatgtacgc gcccgacaac aacgtgctga
    17281 cccccgagac gccgccgatg ccgatccgcg gccgctcgac ccagaaccgc gagtccttgg
    17341 ccgcgaggct ggccgcgccg cggtcgagct cgaccttgac gaggatcgcg cgctgatctt
    17401 cggtgagctc gacgctgctg atcttgccga ccggaacgtt cttgtaacga acgtccgact
    17461 tgccgacctc gaggccttcg gcgctttcga acgtgatctc gatggtcggg ccggcactca
    17521 gatacgcccg gatcgccagc ccgccggcgg cgaccacggc gaggatcggc acgagccaca
    17581 cggcggacgg ccagccgggc ttgcggcggc ggacgaccgg aaccggcaac tcttcgaatc
    17641 cagggtcccc gcggtcactc acagatacct cgatcccaga tcgcacgtgc atcgaacgac
    17701 gcagacgcga gcatcgtgag gatgacgacg cccgcaaacg gcagcaccgc cggcccgggt
    17761 gtgacccggg cgaactgccc gagctggacc accgcggtga gcagcgcgac cacgaacacg
    17821 tcgagcatcg accagtggcc gatgacctcg agcacgcgat acagccgggt acgcgcgcgg
    17881 ggccgccacg ccgagtgtcg catcgcggac acgacaagga acccgagcgc accgagcttc
    17941 aagactggca ccaggatact cgccgtgaac acgatcgcgg cgagtcccca cgatccccgc
    18001 tcccacagga acttcacccc cgacaggatc gtgtcggcgc gccgctccgg aaactgctcc
    18061 gtatacatga tcgtcagcgc gttcgccggg aagtacagcg ccagggcagc gacgaggagc
    18121 gcgatcacgt acgtcctggc gttgcgcttg cggaaccgca ccgacgcatc gcaccgaggg
    18181 caggcagctc ccgcgtcgtc ctcgaagtcg cacacgcgca ccacgagctc gcagctgtgg
    18241 caggtggcga gccggacgtc gatgccgcgg atcacggcat gacctcgtcg gcgcgccgcc
    18301 acagcgcccc ctgatcgaac gacgacagcg acgcgagtgc cagcgccatg accgcaaccc
    18361 cgaacatgcc ccaccccggc aaggcggtgg cgagcgcggc aagcttgacg accgccacga
    18421 ccacgccgag gaagaacacc tcgatcatgc gccatggacg cagcgcccgc agcagtccca
    18481 tcatggagac gaatgcgagt ggccggaccc acaagcacag cggcacgagc agccacagca
    18541 gcagaccgag ctcgatcgcc ggcgcgacga tcagcgtcag tgtcaatgcg atcgcgacaa
    18601 acggcagctc gctgtcgtag gacgccgcga tcgcgtgcca gagcgtcgcc cgggtctgat
    18661 ggcccccgga cgtcagggtg agcaacggca gcgcgttcgc aacgagaaac gcgatcgcgg
    18721 ccgtgacggt gacggcgagc atcgcaccga ggtcgccatt gccgcggtag atcggcgagt
    18781 cacacgtgac gcatcgcatg gtctcgcggc ccacaggcgc ggcccggcga tgcaccgtgt
    18841 cgcaatagct acagatgacg agctcggccg ccggcacatc gcccgcgagg tgcaagcgca
    18901 gcgccggagc gtctgtggga ccatcgcggc ggagtgccgt gggccgcgct gcagcgagcg
    18961 tgctctcagg ccacagacag cgtgcgcagc cgctcgctac cggcgcaccg tgcgaatttc
    19021 cggcacgcac gtgggtggaa tctggagcag gtgcgacccg cgcgcggttt gggcgctacc
    19081 gcggctcggc gggcgcgcgg accatggcgg cgaggacctc acggtagccg ttcgcgagac
    19141 acgcaccatc gagcaggccg cgctgcgcga ggagatcgtg caacctcggc aggttatggc
    19201 aggggacggt catcagcaga tgatgctcga ggtggtagtt gacccggtgg ggcgcgaaca
    19261 agatgcgctc ccagggactt gccagcaccg tgcgcgtgtt ggtcagcgga tcgccggggt
    19321 cgccggtcag cgcgtgctcg gcgatgctgc ggatccgcat gcacaggctg taggtggtga
    19381 gccaggcccc gacccacagc aggtacagga gcggatgccc ggccgcggcg cacacggcga
    19441 tcaggccgag ctgccacagc acggtaccac ccagctgcgt gcgcgcatcc gccgccgagc
    19501 cgccgccgac aaagctcagc gcgtcgctgc ggccgagcgg attgccccgg gcaagcgcga
    19561 gcaagaagcg agtctgtgcg accagccgct tcacgccggt gacgcccgtg agatcgcgga
    19621 cgaccttgcg gcgcatcgag gcccggctca ccggccagcc gcgggccagg ccaatatcgg
    19681 gatcttcgtc ggtaccggtt cgcgcgtgat gctgcaggtg atacggccgg tagagctcga
    19741 ccgacgcgta caccggatac gctgcgagcc agttggcgca gcggtcgttc catcggcggc
    19801 tccggaacag cgtgcgatgc gccgcctcgt gcatcaggat cgccatgccg agctgacgcc
    19861 cgccgatcac gaacagcgca agcggcacga ccagcggcca gagcaccagg ggcgcgaccg
    19921 cgacggcgac catcgccgcc gcgaccatgc tccagctacc cgcgatgccc agcgcaccac
    19981 gcaggttcga gacctcgcgg agctcgcgaa tctcatcggc ggtgaacgca tcgacccacg
    20041 tgcgcgacat cgcggccagc atagcagacc gcgaagcgca cggacgctcg aagaacaaat
    20101 cgcggaccgc acgtcgcgat ggtagtagga tcgccgtcat gaaagagcat cgaccgggcg
    20161 gcggcacgct ggtgggacgg gcaaccgacg gagcagcggc cgccatgccg ggcaagagga
    20221 cgctgactgg tgacctggag ctcatgcagg gcggcatggc cacgaccgaa ggtggcgtgt
    20281 tgttcagcgg cccggatccg aagctcgctg cggagacttc tatcccgcca aggcgacggc
    20341 gtatgccggc gcggtcaccg agctgaccga tgccgcggcg cgctgggacg gcatcgctgg
    20401 aaacacgaaa gcacatctgc aatgacaccc gggaaggcgc tcttcgtgct cgcggcgtgc
    20461 cacggcgcga cgcaacccgc agcgaggacc cgtacgtcgt cggcgtgcaa cagaatcccg
    20521 acgggacgac caccatgttc ttcggcatca tcacgccgcc gcaggtcagc taatgcgcaa
    20581 atgattgaaa taatcccgat attggccagg ttttcgcgat ctcctggtca gcgcatggcg
    20641 ggtgcaccga gctcgacggg tgcccacccg ctggcgacgg agccacgtag ccgcacgcgg
    20701 tcggcgcggc gggccaccgg ccggcgggag cagcgtgaag ctgtcgatcg acaccaggct
    20761 gcggcacgcg gtgcggacgt tcgagtcgtt gcgtgcgcac cgccggctca ctacgttcac
    20821 cacctcccca tcaacgaggc acagatcaag tccatgtcca agctcttttc cgaattccac
    20881 atcaagggcg tgtcgctcag gaatcgcatc gtggtctcgc cgatgtgtca gtactcggca
    20941 gaggatggtg tgccggacga ctggcatctg gttcatctcg gcgcacgcgc catcggcgga
    21001 gcggcactcg tgatcgcgga agcgacggcg gtgtcgccgg aagggcgcat cacgccgggt
    21061 tgcacgggac tgtggaacga caggcaggcc gaggcctggg cgcccatccc gaggttcgtg
    21121 aagcgtcatg gcgcggtgcc cggcatccag atcgcgcatg ccgggcgcaa ggcatcggcg
    21181 aacctgccgt gggagggcga cgatcacatc ccgtcgagcg atccccgctc gtgggacccg
    21241 ctggggccct cgccggtcgc cttcggcgcg aacctgcccc gcgtgccgca cgcgatgacc
    21301 agggacgaga tcctccgcgt ccagcgcgat ttcgtggcgg cggcgaagcg cgcgctggcc
    21361 gtcggcttcg aatggctgga actgcacttc gcccatggct acctggcgca gagcttcttc
    21421 tcgccgatcg ccaacaagcg caccgacgaa tatggcggca gcttcgagaa ccgtgcgcgc
    21481 ttcctgatcg acacgctgag cgcggtgcgc gcgatctggc ccgagaaata cccgctgacg
    21541 atccggctcg gcgtcaacga cttcaccaag gacagccagc cgctggagga gtccatccag
    21601 ctcgtgaagc agttcaagca tcacgggctc gatctgatcg acgtgagcct gggcttcaac
    21661 acgccggatg tttccggcgt gccgtggggg ccggccttca tggcgccgat cgcccagcgc
    21721 atcaaggatg aggctgcgat cgccaccgcc gtcggctggt tcatcaccga gccgcagcag
    21781 gccgaggaca tgatcgcgaa gggacaggcg gacctcgtga tgctggcgcg caaggagctg
    21841 gacgatccgc actttcccta tcacgcggca aaggcgctgg gcgtcgccag tccgcagaac
    21901 gtgctgccgc cccagtatgc gcactggctg aagcgcagat gaccagcgcg cgaggagtcg
    21961 cgtccccgca gctccgcagg tgggtcgtgc tgctcgatgc catcgatcag caccacccgc
    22021 cgctcgcccc gcgccgaccg cagcgccgcc gcccatccgg ctgccgggtg catgtatgca
    22081 tcggcctcca gctcgcgctg atcgggcaac atggatcgga agggctggac gctctacgcg
    22141 ttgaagctcc tggaggagcc atcctcccgg catgagccac tgccgacaca gagcgcggcc
    22201 ctacgaggag cgccttgagc ggatcttagg acggctcgcg ggtcatcgct gcccccccct
    22261 gacgcaccac gatcgatcct tcgactgcgt cgtgagcaag cccaagggta agtgaggagc
    22321 gtccggcgcc tacctggccc ggcgcaaccg aggcgccgcc gattccgcct gcagcgcccg
    22381 caactccgcc gcgatcgctg cggccgagcg gtacggggtg gagcctcgaa gtgggtccca
    22441 atcagagagg caatcggcta actatcgagc gggagaaggg attcggaccc tcgacgtcaa
    22501 ccttggcaag gggatttccg cccaatgatc cccgacggtt cgcaggcttg acggtgcaga
    22561 gcggtgctgc tcgaagcagg tcggtggtgt gaagtggtgt gggcgttcgc tgtcgaagtg
    22621 gctgcggcat accgtgcatt caccatggcg agcgacctga ccgtgcgcct cggtgacttg
    22681 tcgacctcga cgattccatg agcggaccga tggtgatcct cggtgcgtgc ccaggctggt
    22741 gccgggagct cgaccctcgc gattccggtc cgtgtgccgt aacatgctcg catgacgacc
    22801 gccgccaagc tcctcgccga cgcgctggag cttccaggaa ccgaacgaga ggatctctga
    22861 gtggtatcga cacggccggg ccgccgtcgt tgcaatcgaa tggctggtaa cttctgccat
    22921 cggggattta cagccggcat cgcaagctgc gcgctcgctg cgcgcaccgc agattgcgcc
    22981 gtcatgccga cgcccgagct atcgacgcag atcgccaggc ggcgcacgtt tgcgatcatc
    23041 gccaccccga cgccggcaag accacgctga ccgtgaagct gctgctgttc tgcggcgcga
    23101 tccagctcac cggcgcggcc aaggcccgcg gcgaccggcg gcggcgcagc gactggatga
    23161 aggtcgagcg cggcgcgcgg gatctcggtg accacgtcgg cgacatcggt ccagcacgga
    23221 cccggcggcg ccgtcaccac ttccgggtgg tcacctccgc ctcgatccgc gcgacgagcg
    23281 cgtcgactcc cgccgcgcct gcgatgtacg gcgcgctgcc gggcgtatca gggagcgcgt
    23341 tgtacgcggc gcgcgccgcg cgcatctgat cgttgaagat ccgcgtcgcc ttcggcgcca
    23401 gctccgcctc ggccgaagtc gcgtccgccg cagacgacga cagcttcgcg atctcggtct
    23461 cggcgactct cagcgccttc ttcacctcgc cctgccactc ggtctcccag tggtagtgct
    23521 cgtgaagctg cgtgcgcctg tacgagctgt agttgcccgc gacgatcgtc cggtggtcct
    23581 tcaggtcctt aacgacggcc ttccaattgc ccgcattcac gttcgagagc gcgccgctgt
    23641 cgtcgttcgg cgtcggcgcc gggtagtggt ccaccgtgta gtacaccagt tcgatgcggc
    23701 ccttgccctc gacggtctcg agctggtagc gccacaccga gccgtccttg acgaccttac
    23761 ccgtgaacgt tggcgtgtcc gtgttcgtcg tcgcgggtgc ggtgttcgag cgcgcgccct
    23821 cggtctcggt cagcacgaac gtcggcgtaa catgcttcgg cgtccccgct ccggcgccag
    23881 cgttttcctt cggttccgcc ttgcccttgc cgcccttgtc gtccttgtcg tccttctcct
    23941 cgtgcttcgc cggcgcgacc tccgccttcg cgccacccgc aggcacgggc atctcggcga
    24001 ggttgccacg gcccttcccc gtcgcgccgc ttttcggctc agtcttggac gcggccgcgt
    24061 ccgtgtcgag cgcctgcgtc agtgtcgtct tgcccggcgc gaccgtcggc gtcgccgggt
    24121 gctcccagtt cgagagcgac gactttcctg gacggtactc cctcccgtgg ctcatctctg
    24181 cctccgttca gtgcttgtga taggccagcg tggccgtgca gcgctgctcg ccgatagggt
    24241 tttccttggg tccctggaag tacaggttcg aatacgtggc gcggatctcg tagtcgcctg
    24301 gcgcgagaga cgcgccgtcg agcacgaagc gctcgccgtg cgcggaaccc ggcgcgactg
    24361 ccacgtacgc gtcgggcgac tcgggggaga gcttcggtgt gaactttggc ggattcgtct
    24421 cgtagaccgt cgcgccgtgc gcgtcgacga tctgcacgca cacgaacgcg ccgagcggca
    24481 gcaacacgcg cggtacgcgc aaggaactct tcgcagacgc ggggactgct agtgcgaccg
    24541 cgagcgccat cggctcctcg gacgcgaccg acaactggca caccaggtcg ccattcatgt
    24601 tctgacctct ctgcgggcgc gcgggcggcg acgcgggcgg actgcatgcc gccgctgcga
    24661 tccccacaag cgcgctccgc gccaaacgca tcaccttcac cgtagcggga gtatacgtca
    24721 ctcgctgctg cccagggcga aaccgatccc gatcgccggg ttcttcagat gcccggtgcg
    24781 cgtggcagcc gcgtggcgac agcaacgacg ttcagagcag aaaacccaat aaattatgta
    24841 gcttagagag agcgggagaa gggattcgaa ccctcgacgt caaccttggc aaggttgcac
    24901 tctaccactg agttactccc gcgtggcagg gtgcttttct agcggggcgt cggcggtgtg
    24961 tcaagcgccc ggcccgagaa cgtcgggcga ggcggcgctc ccggcgacgg cccccgccga
    25021 cggggctcgg cggatggtga ggcgggcgcg ggggctcacc aggcgtaggc gatgccggtg
    25081 gtcagaccga ggccgtccca gccgcgctga gcgacggcgg tctcgaggct ggcgcgcagc
    25141 cggaggtgct tgccgccgag gtcgaggccg aggcgcgcga tcgcggcggc gaggtcggcg
    25201 cgcgtgttgc ggtagtcggt gaccaggcgc tcgtggcggt agccggcgtc gaggccgctg
    25261 accaggcagg cgatgccgtc gagggcgcag ccacgcgcct cgaggccgac gcgggcgtcg
    25321 gtgaagtcgc tggtcatcgt ggtctcctcg atgacgccca tgccgccgtg ggcgagccgg
    25381 gcgtggagcc agatcgggcc gtcgccgagg cgatgaccgc cgtcgagctg cagcgcgccg
    25441 tagaggccgg cggtggcgag gccgacgtcc aggccgggct cgaggtacga gcgggcggcc
    25501 gtgggatcgc tcgacggcgt cgcgggctcg acgagcgacg gctgggcgtg gacgacggcc
    25561 ggggtcgcga gcagcgaggc gaaggcagcg gcggcggcgt aggaagcggt gcgcatggcc
    25621 agaccagcag gcaaaggtcg gaccattcgc caaccagcgg atcccacgct gaaatcgccg
    25681 agcaaaccgg cgggggtgcg tcgaaggttc accaggctgt ccgaacggag gaccaccggc
    25741 ggcggtgagt tgacacgcac gcggcggtgg cgacactgcg gcatgcgcat cgtttctccg
    25801 gaaagcgtgg ggctgtcgag cgagcggctg cggcgcgtgg atcagcacct gatgtcgcgc
    25861 tacatcgagc cgggcaagat cgccggggcg ctgacgctgg tggcccgaag gggcgaggtg
    25921 gcgtggctgt cgccgctggg gcagatggac cgcgagcgga agaagccgat gcgcgcggac
    25981 acgctgttcc ggatctactc gatgaccaag ccgatcacgt cggtggcgct gatgatgctg
    26041 cacgagcgcg gggtgttcgc gctcggcgat ccggtgcacc gcttcatccc ggagtgggag
    26101 gggttgcggg tgtatcgcta cgggaagcat cccagctggg tgaccgatcc ggcggcgcgg
    26161 ccgatgacga tgcgcgatct gctgagccac cagtcggggc tgacgtatca catcatggag
    26221 cgctcgccga tcgacgaggc gtaccgccag ctcgaggtcg gcgcgccggg caagacgctg
    26281 cgcgagatga tcgagcagct ggcggggctg ccgctggagt tctcgcccgg gacgcgctgg
    26341 gggtactcgc tggcgaccga tgtcatcggg cacctggtcg aggtgctgtc ggggcagcgg
    26401 ttcgacgact acctgcgcac gcagatcttc gagccgctcg ggatggtgga cacgacgttc
    26461 acggtgccgg ccgacaagct ggggcggttc gcggcgaact acacgcggct gccggggagc
    26521 accgaggcga cgctgatgga cgacccgatg gacagcccgt tcgcgcggcc caaggcgatg
    26581 ctgtcggggg gcggcgggct ggtgtcgacg gcggcggact acttccggtt ctgcgagatg
    26641 ctgcggcggg gcggcgagct cgacggcaag cggctgctcg ggccgcggac ggtggcgtac
    26701 atgacgcgca accacctgcc ggacggacgc gacctggcga gcctggccac cgcggggagc
    26761 ttcagcgaga cgcgctacga ggggatcggg ttcgggctcg ggttctacgt ggtggtcgac
    26821 ccggtgcggg cgcagacgcc gacgtcgccg ggagagtacg gctggggcgg gatggcgtcg
    26881 acggcgttct ggatcaaccc ggccgatgac ctggtggtgg tgttcctgac ccagctggtg
    26941 ccgtcgacga cgttcgactt ccgcgggcag ctgaaggcga tcgtccacgc ggcgatcgtc
    27001 gactgagccg gccgcgggct actgcaggcc gccgccgagc acgcggtaca gggtgacgcg
    27061 gttgccgagc gcggcgagct gggtcgcgat cagcgagttc tgggcggtgt acagcgcgcg
    27121 ctgcgagacc agcgtggtca ggaaggtgtc gacgccggcc ttgtagcggg cctcggcgac
    27181 ctcgtagccc ttggtggcgg cgtcgacgag ggcggcctgg gcggcgagct gctcgtcgat
    27241 cgtgccgcgg gtggccagcg catcggcgac ctcgcggaac gcggtctgga tcgcgagctc
    27301 gtaggacgcg atcagctgcc gcttctgggc ctcggcgagc tcgaggttgg cgcggttggc
    27361 gccgccgcgg aacagcggca gggccaggct cggggcgagc gtccacaccg cggccgggcc
    27421 ggtgaacagc gcggtcagcg cggcgctggc cagcccgccg ctcgcggtca gggtgagcga
    27481 cgggaagaac tgcgcgcggg cggcgccgat gttggcgttg gccgcgatca ggtcgtgctc
    27541 ggcggcgagc acgtcggggc ggtcgagcag caccgccgac gccaggccga ccgggacgtc
    27601 ggagaaccag tcgagctgcg cgggcagcgc gtcggggagc agcgcgtcgt cgacatggcc
    27661 gccggcgagc aggtcgagcg cgttgcggtc ctgcccgatc gccgcggtga gcagtgcgac
    27721 gtccgagcgc gcctgctggt agacggtggc ggcctgccag tagtcgccgc ggcccgccgc
    27781 gccgccggtg accagctgct cggtgaggtc catggtctgc cgggtgatct ccatggtgcg
    27841 ctgggcgatc gcgagccggc tgcgatcggc ggcgagcgtg aggtaggcgc tggcggtctc
    27901 ggcgatcagg ctgatccgcg cggcgcgggc ggcctcgacg gtcgacaggt aggactggag
    27961 cttggcgtcc gacaggctgc gcagccggcc gaacacgtcg atctcccacg ctgcgaggcc
    28021 ggcctgcgcg gagtacagcg cgccggtcgc cgtggcgttg ccggggccga cgatcgagcg
    28081 cccgccgttg acctgggcga tcgcgtcgac cgacggcagc gcctgggcgc gctggatccg
    28141 gtactgggcg cgcgccgcct cgatctggag cacggccttg cgcagatcgc ggctctgggc
    28201 gagcgcctgg tcgaggatgt gctgcaggcg cggctcgcgc acgaacgcgc gcaacggcag
    28261 cgcggccgcg gtggcggtgc ccccagaggc tgtaccgccg ggcaggctcg ccgggaccgg
    28321 cgcggcgggg cgctggtagg ccggcgccat cgagtggcag ccgccccaca gcacgaagcc
    28381 ggcggcgagc gccggcgcga ggatcgtgcg cttcataccg ggacccgctt accacgcacc
    28441 agccggcgca caatgacgaa gaacaccggg acgaagaaca ccgcgatcac ggtgccggtg
    28501 agcgttccgc cgacgatgcc ggtgccgatc gacacccggc tgttggcgcc cgcgccagtc
    28561 gacagcgcga gcggcatcac gccggcgacg aacgcgagcg acgtcatcag gatcgggcgc
    28621 agccggagcc gcgcgccggt gaccgcggcg tcgatcaggc tcgcgccgcg gtggtacgcc
    28681 gcctccgcga actcgacgat caggatggcg ttcttcgacg acaggccgat cgtggtcagc
    28741 agcgcgaccc ggaagtacac gtcgttctcc agaccgcgca gcgtcgcggc gagcaccgcg
    28801 ccgacgatgc cgagcgggat cacgagcagc accgagaacg gcaccgacca gctctcgtag
    28861 agcgcggcga ggcacaggaa gatcacgagg atcgagatcg agtacagcgc gagggtctgg
    28921 ccgctcgcga gctgctcctg gtaggacagc ccgctccacg acgaggtcgt gccggggacc
    28981 gtccgggcga ggcgcgcgat ctcgtccatc gcctcgccgg agctgacgcc cggggcgccc
    29041 atgccctgga tgtcgtacga cgccaggccg ttgtagcgcg acagcgactc ggcgccgaac
    29101 gtccaccgcg tggtcgcgaa cgcgctgaac ggcgtcatcg cgcccgaccc gccgcgcacg
    29161 taccacgcgt cgagatcctc gggcttgctg cggaacggcg cgtcgccctg gacgaacacg
    29221 cgcttgaccc ggccgcgatc gatgaagtcg ttgacgtaca cgccgcccca cgccgccgcc
    29281 agtgtgcggg agatgtcggg cggcgcctcg ccgtgcgcga ccgccttggc ctggtcgatg
    29341 tcgatatgga gctgcggggt ctcgggcaga tcgccgcgcc ggaccgcgat cagctttggg
    29401 tccttggacg cggccgcgag cagctggtcc cgggcctgcg cgagcgcggc gcggtcgatg
    29461 ccggcggcct ggagctggaa gtcgaagccc tgcgagttgc ccaggccctg gatcgccggc
    29521 gggg
//
LOCUS       DASVOR010002128         5005 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_52491,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002128 DASVOR010000000
VERSION     DASVOR010002128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52491"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF516_39545"
     CDS             complement(<1..304)
                     /locus_tag="VF516_39545"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HEX8113912.1"
                     /translation="MSVDHGAASWTEACRRRAIVLAVRPEVDGGRFPVKRALGEPLAI
                     EADIVADGHDVVRAVVLDRAPGSAPGATGWRETELVLGPDDTWRATIELAGLGRHRY"
     gene            628..2319
                     /gene="treZ"
                     /locus_tag="VF516_39550"
     CDS             628..2319
                     /gene="treZ"
                     /locus_tag="VF516_39550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HEX8113913.1"
                     /translation="MEAVRIWAPNARRVEVIRGESRTSAERETGGWWRGPTLAAGDRY
                     AISLDGGPPRPDPRSPWQPDGVHAASCWIDPRTLASPAPLGFSPRPLRDAVIYELHVG
                     TFSPRGTFVAAAEHLDHLVQLGVTHVELMPIAQWAGRHGWGYDGVDLFAPHNAYGGPS
                     GLHYLIRECHRRGLAVLIDVVHNHLGPDGAYLPEFGPYLTARHRTAWGDAINLDGDGA
                     PEVRRFLIDSALHWLCDHGVDGLRLDALHALRDDSPHHFVAQLTAEVRAAGARLGRRF
                     RLIGEYDDHDPCAVTPEAEGGWGLDAHWNDDFHHALHALLTGERDGYYGDFAARGTLA
                     KVLRHGYALDGGISNYRGGPHGKPYGALPRDRLVAYTQSHDQVGNRGAGERLHQLAGL
                     DRARIAAAILMTSPFVPMLFQGEEWAASTPFCYFAELESEELRRAVREGRRAEHAAAG
                     WTGQVPDPTDPATRDASVLMWDELTRPPHTEMLAWYRALITARRECPELRAPEPASIE
                     VTEPPGGLDIRRGPYTLRVNLSDAPARPASGTCVLASRPLVAGALPPVSCALFQR"
     gene            complement(2332..3630)
                     /locus_tag="VF516_39555"
     CDS             complement(2332..3630)
                     /locus_tag="VF516_39555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113914.1"
                     /translation="MLVLLVAAFGGVTGADPKREVPDYDGRGNPDAEGGSWALWIPRI
                     VLSPLYVANEYVLRRPLGVLIAHAERARWADTVMEIFTFGEGDHNLIVPTALYDFGLL
                     PCVGFYYAGDQMFVADNQLRIHAATWGSRWINATAADRYLLDQSDRVQARFEFKRSED
                     NLFVDLGPDGKHATESRYGLERVEGSAGYRRTLPGASRIDVEAGVHRISFVEGECCGD
                     PSLDTEIAHGQVMEPPGYRTPYTAGFGRIALTLDSRRERPDPGGGAFLHLEARPSVDL
                     HDERSWLAYSGAAGAAIDLTGHRRTLRASLAASFVDSMTGGAVPFVEYPVLGGDAMAG
                     FITGWMTGPSTAVGEVAYTWPAWLWLDAQTRLAVGNAFGERLDGFAARKLRMSADFGF
                     TTSTAHEQGFELVLGVGTETFEQGAHVTSVRVAVGSRRGF"
     gene            3878..4855
                     /locus_tag="VF516_39560"
     CDS             3878..4855
                     /locus_tag="VF516_39560"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEX8113915.1"
                     /translation="MTRSRPALRTPTVIALLLGVATARAAPDAANTAPPAADAGAPIA
                     PPAPDAPPEKVEEAKKVAQTAALTPIVPNPHDATHPAFLLYVEIDPPLLATGAVFALA
                     RGYKTQPAFCAPACDSVQLNALDKLTAGYYSAAWSTASDYMLYGLGAGTATALIADEG
                     VLDALNDGVVIGEATLSATAVASIMTLAAGRPRPFLYGDPTVPSGYKAPLGIRNGADA
                     SLSFLSSHTAEAFAMVTSLYIAEHRLHPRSSYPKYLLGVGLGVASMIGVARVMSGYHF
                     ITDAVGGAVVGSSVGVLVASIHSSPVHIVPVVDHRPEGNATGVALSGGF"
BASE COUNT          700 a         1925 c         1728 g          652 t
ORIGIN      
        1 tgtagcggtg gcggccgagc ccggcgagct cgatcgtcgc ccgccaggtg tcgtcgggcc
       61 cgagcacgag ctcggtctcg cgccagccgg tcgcgcccgg ggcgctgccg ggggctcgat
      121 cgagcaccac cgcgcgcacg acgtcgtggc cgtcggcgac gatgtcggcc tcgatcgcga
      181 gcggctcgcc gagcgcgcgc ttgaccggaa accgcccgcc gtcgacctcg ggccggaccg
      241 cgagcacgat cgcgcgccga cgacacgctt cggtccagct cgcagcaccg tgatcgacgg
      301 acattcgttt ccagggtagc gactgcgcgg aagcgtccaa cgccggactc gacggacctt
      361 accgctgcct caccaccgcg ccacgccaac ggccacgctg cgacatcgcg tcgatcggag
      421 atccacgcag cggccgcgcg cgatcgcgcg caccgagcga acccgcacag cgcattgtcg
      481 agaatccgca cggctgccga ccgagcgccg gcggccggcg gccggcatgg cgccgtcccc
      541 gacgacggcg actccatcgt ctccaccggc ggcggaccgc gccggaacag cgtgaccgcg
      601 gcacgacgct cgcacccggc cggcaccatg gaagcagtac ggatctgggc accgaatgcc
      661 cgacgcgtgg aggtgatccg cggtgaatcc cggaccagcg ccgagcgcga gaccggcggc
      721 tggtggcgcg gcccgacgct ggccgccgga gaccgctacg cgatcagcct cgatggcgga
      781 ccgccgcggc ccgacccacg ctcgccgtgg cagcccgacg gcgtgcacgc cgcgtcgtgc
      841 tggatcgatc cccgcacgct ggcgtcgccg gcgccgctgg ggttctcgcc gcggccgctg
      901 cgcgacgcgg tgatctacga gctgcacgtc gggacgttca gcccgcgcgg cacgttcgtc
      961 gccgcggccg agcacctcga tcacctggtc cagctcgggg tcacccacgt cgagctgatg
     1021 ccgatcgcgc agtgggccgg ccggcacggc tggggctacg acggggtcga cctgttcgct
     1081 ccgcacaatg cgtacggcgg accctccggg ctccactacc tgatccgcga gtgccaccgg
     1141 cgcgggctcg cggtgctgat cgacgtcgtc cacaaccacc tcggccccga cggcgcctac
     1201 ctgccggagt tcgggccgta cctgaccgcg cgccaccgca ccgcgtgggg cgacgcgatc
     1261 aacctcgacg gcgacggcgc gcccgaggtc cggcggttcc tgatcgacag cgcgctgcac
     1321 tggctgtgcg atcacggggt ggacgggctg cggctcgacg cgctgcacgc actgcgcgac
     1381 gacagcccgc atcacttcgt cgcacagctc accgccgagg tccgcgccgc gggcgcacgg
     1441 ctcggccggc gattccggct gatcggcgag tacgacgacc acgatccgtg cgcggtcacg
     1501 cccgaggccg agggcggctg gggcctcgac gcgcactgga acgacgactt ccaccacgcg
     1561 ctccacgccc tgctcaccgg cgagcgcgac ggctactacg gcgacttcgc cgcgcggggc
     1621 acgctcgcca aggtgctgcg ccacggctac gcgctcgacg gcggaatctc gaactaccgc
     1681 ggcggcccgc acggcaagcc gtacggcgcc ctgccgcgcg atcggctggt cgcctacacg
     1741 cagtcgcacg atcaggtcgg caatcgcggc gccggcgagc gcctgcacca gctcgccggc
     1801 ctcgatcgcg cccggatcgc cgccgcgatc ctgatgacct cgccgttcgt cccgatgctg
     1861 ttccagggcg aggagtgggc cgcgtcgacc ccgttctgct acttcgccga gctcgagtcc
     1921 gaggagctcc gccgcgccgt gcgcgagggt cgccgcgccg agcatgccgc cgccggctgg
     1981 accggccagg tgcccgaccc gaccgatccg gcgacccgcg acgccagcgt gctgatgtgg
     2041 gacgagctga cccggccgcc gcacaccgag atgctcgcgt ggtaccgcgc gctgatcacc
     2101 gcgcgccggg aatgccccga gctccgcgcc cccgagccgg cctcgatcga ggtcaccgag
     2161 ccgccgggcg gcctcgacat ccgccgcggc ccgtacacgc tgcgggtcaa cctgtccgac
     2221 gcgccggcgc gccccgccag cggcacctgc gtgctggcgt cgcgaccgct ggtggccggc
     2281 gcgctgccgc cggtgtcgtg cgcgctgttc cagcgctgag cgcgccgccg gtcagaaccc
     2341 gcgccgcgat ccgactgcga cccgcaccga ggtcacgtgc gcgccctgct cgaacgtctc
     2401 ggtgccgacg ccgagcacga gctcgaaccc ctgctcgtgc gccgtgctgg tcgtgaaccc
     2461 gaagtcggcc gacatgcgaa gcttgcgcgc cgcgaagccg tcgagtcgct cgccgaacgc
     2521 gttgccgacc gccagccggg tctgggcgtc gagccacagc cacgccggcc aggtataggc
     2581 gacctcgccc accgcggtgc tggggccggt catccagccg gtgatgaacc ccgccatcgc
     2641 gtctccgccg agcaccgggt actcgacgaa cgggaccgcg ccgccggtca tcgagtcgac
     2701 gaagctggcc gcgaggctgg cgcgcagcgt ccggcggtgg ccggtcaggt cgatcgcggc
     2761 gccggccgcc ccgctgtagg ccagccacga ccgttcgtcg tggaggtcga cgctcggccg
     2821 ggcctcgagg tgaaggaacg cgccgccacc ggggtccggc cgctcgcgcc gcgagtcgag
     2881 cgtcagcgcg atccggccga agccggcggt gtagggcgtg cgatagcccg gcggctccat
     2941 gacctggccg tgcgcgatct cggtgtcgag cgacgggtct ccgcagcact cgccctcgac
     3001 gaagctgatc cggtggacgc cggcctcgac gtcgatccgc gacgcgccgg gcagcgtgcg
     3061 ccggtagccg gcgctgccct cgacgcgctc gaggccgtag cgcgactcgg tggcatgctt
     3121 gccgtcgggc ccgaggtcga cgaacaggtt gtcctcggag cgcttgaact cgaaccgcgc
     3181 ctgcacgcga tcgctctggt cgagcaggta gcggtcggcg gccgtcgcgt tgatccagcg
     3241 cgacccccag gtcgcggcgt ggatccgcag ctggttgtcc gcgacgaaca tctggtcgcc
     3301 ggcatagtag aagccgacgc acggcaacag gccgaagtcg tacagcgcgg tcgggacgat
     3361 cagattgtga tccccctcgc cgaacgtgaa gatctccatc accgtgtcgg cccagcgcgc
     3421 gcgctccgca tgtgcgatca gcaccccgag cggccggcgc aacacgtact cgttggccac
     3481 gtacagcggc gacagcacga tccgcgggat ccacagcgcc cacgagccgc cctcggcgtc
     3541 cgggttgccg cggccgtcgt agtccggaac ctcgcgcttc ggatcggcgc cggtgactcc
     3601 gccgaacgcc gcgaccagca gaaccagcat gctgcaaagc gttgtacgtg gagtgcgaat
     3661 gacccaccac cgtgcaaggt ccccgccagt cgttcggccc ggaacgtctc gcaagcgccg
     3721 gacccgaacg tgcggaattg ccacatcgag cacgcgatcg ggtcagggaa gatcggttgt
     3781 ccgcactccg ccgcatcgca gcgaaccgga tcgccctgcg aacgtgcgtg aacgagccgg
     3841 tcgcggctgg cctccgaatt tgcaagggac gctgttcatg acacgttccc ggccagcact
     3901 ccggaccccg accgtgatcg ccttgctgct cggcgtcgcc accgcccgcg cggccccgga
     3961 tgccgcgaac acggcgccgc ccgcggccga tgccggcgcg ccgatcgccc cgcccgcccc
     4021 ggacgccccg cccgagaagg tcgaggaggc caagaaggtg gcccagaccg cggcgctgac
     4081 cccgatcgtt cccaatccgc acgacgcgac gcacccggca ttcctgctct atgtcgagat
     4141 cgatccgccg ctgctcgcga ccggcgcggt gttcgcgctg gcccgtggat acaagaccca
     4201 gcccgcgttc tgcgccccgg cgtgcgacag cgtgcagctc aacgcgctcg acaagctgac
     4261 cgccggctac tacagcgccg cgtggtcgac cgcgtcggac tacatgctgt acggcctggg
     4321 cgcgggcacc gccaccgcgc tgatcgccga cgagggcgtg ctcgacgcgc tcaacgacgg
     4381 cgtggtgatc ggcgaggcga ccctcagcgc caccgcggtc gcctcgatca tgacgctggc
     4441 cgccggccgg ccccgcccgt tcctgtacgg cgatccgacc gttcccagcg gctacaaggc
     4501 gccgctcggc atccgcaacg gcgccgacgc cagcctgtcg ttcctgtcga gccacaccgc
     4561 cgaggcgttc gcgatggtca cctcgctgta catcgccgag caccgcctgc acccccggtc
     4621 gagctacccc aagtacctcc tcggggtcgg gctcggcgtc gcctcgatga tcggcgtcgc
     4681 gcgcgtgatg tcgggctacc acttcatcac cgacgcggtc ggcggcgccg tggtcggctc
     4741 gtcggtcggc gtgctggtcg cctcgatcca cagctcgccg gtccacatcg tgccggtcgt
     4801 cgatcaccga cccgagggca acgccaccgg cgtcgcgcta tccggcgggt tctagtcagc
     4861 ggacgctcgc gggatcggca agctcgtggt gcaggtcgag cgcctgcgcg tggcgatgcg
     4921 cggcaccgag cgttaccctc gcgctgcagc cgtcggcagc cgcgcgctgc cgtgaccgtc
     4981 cgccgagatc acgcgatcgc agggc
//
LOCUS       DASVOR010002129         1537 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_284796,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002129 DASVOR010000000
VERSION     DASVOR010002129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284796"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1537
                     /locus_tag="VF516_39565"
     CDS             <1..>1537
                     /locus_tag="VF516_39565"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8113916.1"
                     /translation="LLPTPVQTAVYRPVSEGKDLLVQSRTGTGKTTAFGLPTISKIIP
                     THRAPQALVLAPTRELALQVSRELTQLGKHRGILVEPIYGGAPIGKQIRALRDGVHIV
                     VGTPGRVLDHIGRRTLDCSTVQTFILDECDEMLSMGFLEDIERVVSYLPDRRQTLLFS
                     ATMPDEVLRYARRHMRSPEQISLSRDGISVTEIHHAYYIVSGIARSRDLLKIIFAENP
                     ESAIIFCNMRDETTMVARFLQKQGLDAEPLSSDLAQSDRERVMNRMKQHNLRFLCATD
                     VAARGIDISNLSHVINYSVPETPEIYVHRTGRTGRAGKKGTALSLVGPRELGNFRYVR
                     LQFGIKPEERVLPKDDIFIGRLKVPLPPVGTPTPPDPVQILIRGTALAHGGPTDLEMK
                     IFEKLMVTQAGKKVAAQLVAERLNQLTTRSKPRRPERSERPERDREDEAAGAPEERSE
                     RGERGERRFDRDRGERRFDRDREDRGERRFDRDRPDRGERGERPWRDRDETRRDRPDR
                     GDRG"
BASE COUNT          254 a          565 c          527 g          191 t
ORIGIN      
        1 acctgctgcc gaccccggtc cagaccgcgg tgtaccggcc ggtcagcgag ggcaaggacc
       61 tgctggtcca gtcgcgcacc ggcaccggca agaccacggc gttcggcctg ccgacgatca
      121 gcaagatcat cccgacgcac cgggcgcccc aggccctggt cctggcgccg acccgcgagc
      181 tcgcgctgca ggtgtcgcgc gagctgaccc agctcggcaa gcaccgcggc atcctggtcg
      241 agccgatcta cggcggcgcg ccgatcggca agcagatccg cgcgctgcgc gacggcgtgc
      301 acatcgtcgt cgggacgcct ggccgcgtgc tcgatcacat cggccgccgc acgctcgact
      361 gcagcacggt gcagaccttc atcctcgacg agtgcgacga gatgctgtcg atgggcttcc
      421 tcgaggacat cgagcgcgtc gtgtcgtacc tgccggaccg ccggcagacc ctgctgttct
      481 cggcgacgat gcccgacgag gtcctgcgct acgcgcgccg ccacatgcgg tcgcccgagc
      541 agatctcgct gtcgcgcgac ggcatctcgg tcaccgagat ccaccacgcg tactacatcg
      601 tcagcggcat cgcgcgcagc cgcgatctgc tcaagatcat cttcgccgag aacccggaga
      661 gcgcgatcat cttctgcaac atgcgcgacg agaccaccat ggtcgcgcgg ttcctgcaga
      721 agcagggcct cgacgccgag ccgctgtcga gcgacctggc gcagagcgat cgcgagcgcg
      781 tgatgaaccg gatgaagcag cacaacctcc ggttcctgtg cgcgaccgac gtcgccgccc
      841 gcggcatcga catctcgaac ctgtcgcacg tcatcaacta ctcggtgccc gagacgcccg
      901 agatctacgt ccaccgcacc ggccgcaccg ggcgcgccgg caagaagggc accgcgctgt
      961 cgctggtcgg gccgcgcgag ctcggcaact tccgctacgt ccggctccag ttcgggatca
     1021 agcccgagga gcgggtgctg cccaaggacg acatcttcat cggccggctc aaggtcccgc
     1081 tgccgccggt cggcacgccg acgccgccgg atccggtgca gatcctgatc cgcggcacgg
     1141 cgctggccca cggcggcccg accgatctcg agatgaagat cttcgagaag ctgatggtca
     1201 cgcaggccgg caagaaggtc gccgcgcagc tggtcgccga gcggctcaac cagctgacca
     1261 cgcggagcaa gccgcggcgt cccgagcgca gcgagcggcc ggagcgcgat cgcgaggacg
     1321 aggccgccgg cgcccccgaa gagcgcagcg agcgcggcga gcgcggcgag cgcaggttcg
     1381 accgcgatcg cggcgagcgc aggttcgatc gcgatcgcga ggaccgcggc gagcgcaggt
     1441 tcgaccgtga ccggccggac cggggcgagc gcggcgagcg cccgtggcgc gaccgcgacg
     1501 agacccgccg cgatcgcccg gaccgtggcg accgtgg
//
LOCUS       DASVOR010002130         2123 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_490534,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002130 DASVOR010000000
VERSION     DASVOR010002130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490534"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VF516_39570"
     CDS             <1..248
                     /locus_tag="VF516_39570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113917.1"
                     /translation="KDEVQTRMRELIARKVEGQEITVVPEAPSGRVIDLMEALKASLG
                     MTKEDRKPAAAGHRIPAAAPAAEAPAEAPKKKAAKKK"
     gene            250..1254
                     /locus_tag="VF516_39575"
     CDS             250..1254
                     /locus_tag="VF516_39575"
                     /inference="COORDINATES: protein motif:HMM:NF013328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEX8113918.1"
                     /translation="MPELPDVTVYVERLGALVIGRPLEAIRIASPFVLRTVSPSPGEL
                     RGATVASVERLGKRIVIGVAGERFLVLHLMIAGRLRWKPRGERLPAKVGLAAFDFPGG
                     TLVFTEASPRKRASLHLVAGRAGLAEFDRGGLDVLTATPAAFGARLRSERHTVKRALT
                     DPTLFDGIGNAYSDEILHAARLSPFRMTPSLGDDEIAALHRACVATLTEWTQRLRDEV
                     GDGFPDKVTAFRPEMAVHGKYGQPCPVCGTKVQRIVYADNEANYCPTCQTEGRLLADR
                     SLSRLLKEDWPRSLEELEERKAQRVAPAAPAATPPPTSPPAPGSPPARRRSGNKSRAS
                     "
     gene            1335..>2123
                     /locus_tag="VF516_39580"
     CDS             1335..>2123
                     /locus_tag="VF516_39580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113919.1"
                     /translation="MNKRLAGFLLAAAAAGCGPKHASFVKVDEVALGRVVIYRNGVAF
                     YERRATVAGGTLTVSVPRDRVDDFLKSLTVVDAISRTPLPVSIPRQQTEDGTNLVMKL
                     ELPSKNTAEVVLTYVTESPAWKPSYRVVVGDHKDVMLEGWAIVDNTSGEDWKNVYVGV
                     GSSSALSFRYDLWSVRQVQRETLANEERFAIAPPSGVSPYGSTRPGESGDVLLSELGD
                     DEIRRPTGHPGDARAHRLATKDDSMLSSEELANLAEQEAKAEAIT"
BASE COUNT          312 a          777 c          758 g          276 t
ORIGIN      
        1 acaaggacga ggtccagacc cggatgcgcg agctgatcgc ccgcaaggtc gagggccagg
       61 agatcacggt ggtgcccgag gcgccgagcg gccgggtcat cgatctcatg gaggcgctca
      121 aggccagcct cggcatgacc aaggaggacc gcaagccggc ggccgcgggg catcgcatcc
      181 cggcagccgc gccggcggcg gaggcccccg ccgaggcccc caagaagaag gcggccaaga
      241 agaagtagga tgcccgagct ccccgacgtc acggtctacg tcgagcggct gggcgcgctg
      301 gtgatcggcc ggccgctcga ggcgatccgg atcgcgagcc cgttcgtgct gcgcacggtg
      361 tcgccgtcgc ccggcgagct gcgcggcgcc acggtcgcgt cggtcgagcg gctcggcaag
      421 cggatcgtga tcggcgtcgc cggcgagcgc ttcctcgtcc tccacctgat gatcgccggc
      481 cggctgcgct ggaagccgcg cggcgagcgg ctgccggcca aggtcgggct cgccgcgttc
      541 gacttccccg gcggcacgct ggtgttcacc gaggcctcgc ccaggaagcg cgcgtcgctc
      601 cacctggtcg cggggcgcgc cgggctcgcc gagttcgatc gcggcggcct cgacgtgctc
      661 accgcgaccc ccgcggcgtt cggcgcccgg ctgcgctccg agcgccacac ggtcaagcgc
      721 gcgctgaccg atccgacgct gttcgacggc atcggcaacg cgtactccga cgagatcctc
      781 cacgcggcgc ggctgtcgcc gttccggatg acgccgtcgc tcggcgacga cgagatcgcc
      841 gcgctgcacc gcgcctgcgt cgccacgctg accgagtgga cccagcggct gcgcgacgag
      901 gtcggcgacg gcttccccga caaggtcacc gcgtttcgcc ccgagatggc ggtccacggc
      961 aagtacggcc agccatgccc ggtgtgcggc accaaggtcc agcgcatcgt ctacgccgac
     1021 aacgaggcca actactgtcc gacctgccag accgaaggcc ggctgctcgc cgatcgcagc
     1081 ctgtcgcggc tgctcaagga ggactggccg cgcagcctcg aggagctcga ggagcgcaag
     1141 gcccagcgcg tcgccccggc ggcaccggcc gcgaccccgc cgcctacatc gccgcctgcg
     1201 ccaggcagcc ctcccgctcg ccggaggtcc gggaataaat cgcgcgcgtc gtagacccgg
     1261 ccgggtggcc gtgcgtttgg cgatccagcg tctcgtcgcg cggcggtcgc gcggcgtctc
     1321 acgccagggg tgccatgaac aagcgtctcg ccgggtttct gttggccgcg gccgcggccg
     1381 gttgcggtcc caagcatgcc tcgttcgtca aggtcgatga ggtcgcgctc ggccgcgtcg
     1441 tgatctatcg caacggggtc gcgttctacg agcgccgggc gaccgtcgcc ggcggcacgc
     1501 tcacggtcag cgtgccgcgc gaccgggtcg acgacttcct gaagtcgctg accgtggtcg
     1561 atgcgatctc gcgcacgccg ctgccggtct cgatcccgcg ccagcagacc gaggacggca
     1621 ccaacctggt gatgaagctc gagctgccga gcaagaacac cgccgaggtg gtcctgacct
     1681 acgtcaccga gtcgccggcg tggaagccga gctaccgcgt ggtggtcggc gaccacaagg
     1741 acgtcatgct cgagggctgg gcgatcgtcg acaacacctc gggcgaggac tggaagaacg
     1801 tgtacgtcgg cgtcggctcg tcgtcggcgc tgtcgttccg ctacgacctg tggagcgtgc
     1861 gccaggtcca gcgcgagacc ctggccaacg aggagcggtt cgcgatcgcg ccgccgtccg
     1921 gggtgtcgcc gtacggctcg acccggccgg gcgagtccgg cgacgtcctg ctcagcgagc
     1981 tcggcgacga cgagatccgc cgcccgaccg gccaccccgg cgacgcccgc gcccaccggc
     2041 tcgccaccaa ggacgactcg atgctgagca gcgaggagct cgccaacctc gccgagcaag
     2101 aggccaaggc cgaggccatc acg
//
LOCUS       DASVOR010002131         1568 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_168679,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002131 DASVOR010000000
VERSION     DASVOR010002131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168679"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="VF516_39585"
     CDS             <1..298
                     /locus_tag="VF516_39585"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX8113920.1"
                     /translation="RALKVHEIQGSTFSVSNLGMFGIERFTAIINPPEAGILAVGAAR
                     DVAVIDTGDKGHVVAGKRMTVSMSCDHRVIDGALGARWLTVLADLIEKPESLAL"
     gene            348..>1568
                     /gene="lpdA"
                     /locus_tag="VF516_39590"
     CDS             348..>1568
                     /gene="lpdA"
                     /locus_tag="VF516_39590"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEX8113921.1"
                     /translation="MIGSGPGGYVAAIRCAQLGLRTACVERENLGGICLNWGCIPTKA
                     LLRSAELLETMHRAKEFGIIAGDIRPDFPAMIQRSRAIADKVSKGVGFLFRKNKIAHL
                     AGTATLLPRTGAWKPHRIQVDAAGGKSVVETKHVIVATGARARSLPGIAIDGERIIEY
                     RKAMTLPAQPRSMVVLGAGAIGVEFASFYRALGVEVTIVEFLPRIVPNEDPEISKELT
                     RAFERRGIQLLAGHKLTSANNAGDRVAMTVEPSAGGEARSLTADLLLVAVGVAANTET
                     LGLEAHGVALDRGFIQVDAENRCGDGLWAIGDVIDRGLAHVASAEGVFVAEKIAGAHA
                     PEVRYDAIPACTYCHPEIASVGLTEDKARAQNIPIKVGRMPFRALAKASAAGDPSGFV
                     KVLWHAETGALVGAH"
BASE COUNT          235 a          586 c          531 g          216 t
ORIGIN      
        1 gcgcgcgctc aaggtccacg agatccaggg ctcgacgttc agcgtgtcca acctcggcat
       61 gttcggcatc gagcggttca ccgcgatcat caacccgccc gaggccggca tcctcgcggt
      121 cggcgcggcg cgcgacgtcg cggtgatcga taccggcgac aaaggccacg tggtcgccgg
      181 caagcggatg accgtgtcga tgtcgtgcga ccaccgcgtg atcgacggcg cgctcggcgc
      241 gcgctggctg accgtgctcg ccgacctgat cgagaaaccg gagtcgctcg cgctgtgacc
      301 cggcgcgccg ccgaggaaag ctcatgtctg acacatacga tgtggtggtg atcggctcgg
      361 gtcccggcgg ctacgtcgcc gcgatccggt gcgcccagct cgggctcagg accgcctgcg
      421 tcgagcgcga gaacctcggc gggatctgcc tcaactgggg ctgcatcccg accaaggccc
      481 tgctgcgctc ggccgagctg ctcgagacca tgcaccgcgc caaggagttc gggatcatcg
      541 ccggcgacat ccgccccgac ttcccggcga tgatccagcg cagccgcgcg atcgccgaca
      601 aggtcagcaa gggcgtgggc ttcctgttcc gcaagaacaa gatcgcgcac ctcgccggca
      661 ccgcgacgct gttgccgcgc accggcgcct ggaagccgca ccgcatccag gtcgacgctg
      721 ccggcggcaa gtcggtggtc gagaccaagc acgtcatcgt cgcgaccggc gcgcgcgccc
      781 gctcgctgcc cggcatcgcg atcgacggcg agcgcatcat cgagtaccgc aaggcgatga
      841 ccctgcccgc ccagccgcgg tcgatggtcg tgctcggcgc cggcgcgatc ggcgtcgagt
      901 tcgcgtcgtt ctaccgcgcg ctcggcgtcg aggtcacgat cgtcgagttc ctgccgcgga
      961 tcgtccccaa cgaggacccc gagatctcga aggagctgac ccgcgcgttc gagcgccgcg
     1021 gcatccagct gctcgccggc cacaagctga cctcggcgaa caacgccggc gatcgcgtcg
     1081 cgatgaccgt cgagcccagc gccggcggcg aggcccgctc gctcaccgcc gacctcctgc
     1141 tggtcgcggt cggcgtcgct gccaacaccg agaccctggg cctcgaggcc catggcgtcg
     1201 cgctcgaccg cggcttcatc caggtcgacg ccgagaaccg ctgcggcgac ggcctgtggg
     1261 cgatcggcga cgtgatcgac cgcggcctgg cccacgtcgc atcggccgag ggcgtgttcg
     1321 tcgccgagaa gatcgccggg gcccacgctc ccgaggtccg ctacgacgcg atcccggcct
     1381 gcacgtactg ccaccccgag atcgcctcgg tcggcctcac cgaggacaag gcccgcgccc
     1441 agaacatccc gatcaaggtc ggcaggatgc cgttccgcgc gctcgccaag gcgtccgccg
     1501 ccggcgatcc cagcggcttc gtcaaggtgc tgtggcacgc cgagaccggc gcgctggtcg
     1561 gcgcgcac
//
LOCUS       DASVOR010002132         4601 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401932,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002132 DASVOR010000000
VERSION     DASVOR010002132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4601)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4601)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4601
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401932"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(193..921)
                     /locus_tag="VF516_39595"
     CDS             complement(193..921)
                     /locus_tag="VF516_39595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113922.1"
                     /translation="MNEILDVLMRIPGVNGAALCNQQGECVLSQLIPPFEPILIKKIV
                     SEVRGVYQTIRYLDDSDPQFLVLHLDDVVVAVRQIEELTVVALATSTTNSTMLSVGFN
                     VVYLKVLDHGIANFELAAGTGPRPGSGSHSPPRPSSRTPTGVPIARSEGTASLSISDS
                     GQPVAPDFIGDELVQRLVKALARQLGPAARLVFKQQVKHIGLTVGMITPNNLPTVVDA
                     LLQRVTDAAKREAFLSDVRALLRR"
     gene            complement(918..1244)
                     /locus_tag="VF516_39600"
     CDS             complement(918..1244)
                     /locus_tag="VF516_39600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113923.1"
                     /translation="MSELDKVLKVSPGLRGIARSNTSGSIEEFTGELDAESVCAVAAM
                     CTQHLKRIEELLGCGELTSWALSTANTGLYVHHGHDGLIAIVGEANKNPDVLLRKTKD
                     AIRGGR"
     gene            complement(1428..1736)
                     /locus_tag="VF516_39605"
     CDS             complement(1428..1736)
                     /locus_tag="VF516_39605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113924.1"
                     /translation="MLVIDSKGLLARGGMLIVSLVRSARAPLDLARLPCTTLLERLAV
                     SRATMQLTLACPHAPALWLSNEPPATMPCPAPNVVCKGWYATVIVRKEPPSTGWSAMG
                     "
     gene            2010..3521
                     /locus_tag="VF516_39610"
     CDS             2010..3521
                     /locus_tag="VF516_39610"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113925.1"
                     /translation="MVENSGSALGKYTLLARLATGGMAEIFAAKRPEGDIVVVKRILP
                     HYAENPQFVQMFLDEARVASRIRHPNVCQVFELGRIGKQYFIAMELLEGVTLARLISR
                     IAKSGRRIDIPVALGIIVQACEGLHFAHELKGFDGRPAKLVHRDISPQNLFVTIDGIA
                     KVLDFGIAKTQTNQAKTLTGMLKGKSGYMSPEQVRGDPLDRRSDVFSLAVVCYETLTC
                     CRLFTRENQFQTYNAILNGTIPALTSTRVDIPSAVSRAVLGGLARTLGERHASCHVFA
                     EALKAAAGAGGIASRMTIKSTMERLFSTEIESQRQMLNDALPELPMSELAVARQPRKG
                     EISELSGLLLDDLGDVGATLLDDLAPIDLALSSDEKATALEDKTSPDDKQPKPVPGSI
                     ARAKTPVPVPEVTPVASARQAVRTVALTAPPPEAPPSSFGLWLVISAGAVVLSAILFV
                     VVWVWVSGHRVEIEVLAPSRAPAMIQNAEPTREEPSPAALDAAVDAGAAIDRP"
     gene            3660..>4601
                     /locus_tag="VF516_39615"
     CDS             3660..>4601
                     /locus_tag="VF516_39615"
                     /inference="COORDINATES: protein motif:HMM:NF023273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8113926.1"
                     /translation="MASRGWGLVPKTILPTIVVSVVIASAVGIVLYLDEKASVIGESR
                     KTAIAIAQQTLAERQVYTANVVNPLLRDFGGPDQGFTTGNNTIHWVTSKMHNPKDKGS
                     LPLPAALVHLVSASVNSRGAKGHTLDLLSLDAVNPEKNPLKTGGSPTEISAMQFLQSE
                     KNPLTYREAVSGEGVDARYVLWYPDIAADACVSCHNGLGGGHKNDWVRGDVMGSIKVT
                     LPMGTDLENAKSGAFRIMLLVLGAFAVVALLQTLLQWLFVSRPLIRNLNMLERAAERI
                     SHGEVDQPIKIESNDEVGRLGQSFERMRTSLVTLLDEA"
BASE COUNT          741 a         1563 c         1545 g          752 t
ORIGIN      
        1 cgtgggtcgc gaccacgccg ccgccgatca cggcgagccg cggcgcgccc ggcctgcgcg
       61 ccagcgagcg agtccgcgcg tcaccagcgt tcacctcgct atcttactcg ggtctcgacc
      121 gacgcgccgt gtcacgcgcg atctccgcgc cgccgctcct ggctgcgctc cccgggtcaa
      181 ggccatcgcg gctcaacggc ggaggagagc tcgtacgtcg gacaggaagg cctcgcgctt
      241 ggcggcatcg gtgacgcgct gcaggagtgc gtcgacgacg gtcgggaggt tgttcggtgt
      301 gatcatgccg accgtgagcc cgatgtgctt gacctgctgc ttgaacacga gcctggcggc
      361 cggtccgagc tggcgcgcga gcgccttgac gaggcgctgg acgagctcgt cgccgatgaa
      421 atccggcgcg accggctggc cgctgtcgga gatgctgagg ctcgcggtgc cctcgctacg
      481 cgcgatcggc accccggtgg gcgtcctgct gctgggccgc ggcgggctgt gcgatccgct
      541 gcccgggcgc ggtccggtcc ccgcagcgag ctcgaagttc gcgatcccgt gatcgaggac
      601 cttgaggtac acgacgttga agccgacgct gagcatcgtg ctgttggtcg tgctcgtcgc
      661 gagcgcgacc accgtcagct cctcgatctg ccggaccgcg acgacgacat cgtcgaggtg
      721 gaggacgaga aactgcggat ccgagtcgtc gaggtagcgg atcgtttgat acacgccgcg
      781 gacctcggag acgatcttct tgatcaggat cggctcgaac ggcgggatca gctggctgag
      841 cacgcactcg ccctgctggt tgcacagcgc ggcgccgttc acccccggga tcctcatgag
      901 cacgtcgagg atctcgttca tcgcccaccc cggatcgcgt ctttcgtctt gcgcagcagc
      961 acatcggggt tcttgttcgc ctcgccgacg atggcgatga gcccgtcgtg gccgtgatgc
     1021 acgtagagcc cggtgttcgc cgtgctcagc gcccacgacg tgagctcgcc gcagccgagc
     1081 agctcctcga tccgcttgag gtgctgcgtg cacatcgccg cgaccgcgca cacgctctcg
     1141 gcatcgagct caccggtgaa ctcctcgata ctcccgctgg tattcgagcg ggcgatcccg
     1201 cgcaaccccg gcgaaacctt caatacctta tcaagctcgc tcatcgcgct accgtctttc
     1261 ttggtcgctg gcggcgccgg tttcgcggaa agcagtggcg tccgcgggtg cggacttggt
     1321 cggcagcggc gccgcgccca gctccgtagc agccgggatg aaccccgtcg aggcgaccgt
     1381 gaacccgtgt tgaagggata ggctccgctc cttggctgtt tcgcccgtca tcccatcgcc
     1441 gaccacccgg tggacggggg ttccttgcgc acgatcacgg tagcatacca acccttgcag
     1501 acgacattcg gcgccgggca cggcatcgtg gccggcggct cgttcgataa ccacaatgca
     1561 ggtgcgtgtg gacaggcaag cgtgagctgc atcgtcgccc tggacactgc gagccgctcc
     1621 agcaaggtag tacaaggcag gcgggcgagg tccagcggcg cccgtgcgct gcgtaccagg
     1681 ctcacgatca gcatgccgcc cctcgcgagc aggcccttgc tgtcgatcac cagcaccgag
     1741 cgcgcgttcg cgtggagcgg cgccggtcct gatatgactc ctggtacaat tccagcgatg
     1801 cggctgcgca gctgggtcgc gccatgtgcg tgcgagttgg tgctcgcgtg tcgcagcaag
     1861 aaccccgacc cggcacaaac caacgccgag ctccgtcgtt ggtcgggtgc cccccaggcc
     1921 gtcggatcgg ggaatgcctc ggtctcccca ccaccctcga ccgacggtgc cgcaccgccg
     1981 tcggatgatg ccccatagcg agcgacgcga tggtcgaaaa ctcgggatcc gcgctcggca
     2041 agtacacact gctggccagg ctcgcgaccg gcggtatggc cgagatcttc gcggcgaaac
     2101 gccccgaggg cgacatcgtc gtggtcaagc ggatcttgcc gcactacgcc gagaacccgc
     2161 agttcgtgca gatgttcctc gacgaggcgc gcgtcgcgtc gcggatccgc catcccaacg
     2221 tgtgccaggt gttcgagctc ggccggatcg gcaagcagta cttcatcgcg atggagctac
     2281 tcgagggcgt gacgctcgcg cgcttgatct cgcggatcgc gaagtcgggc cggcgcatcg
     2341 acatcccggt cgcgctcggc atcatcgtcc aggcgtgcga aggcttgcac ttcgcgcacg
     2401 agctcaaggg cttcgacggc cggccggcca agctcgtcca ccgcgatatc tcgccgcaga
     2461 acctgttcgt cacgatcgac ggcatcgcga aggtgctcga tttcgggatc gcgaagacgc
     2521 agaccaatca ggccaagacg ctcaccggca tgctcaaggg caagtccggc tacatgtcgc
     2581 ccgagcaggt gcgcggcgac ccgctcgatc gacgcagcga cgtgttctcg ctcgcggtgg
     2641 tttgctacga gaccttgacg tgctgtcggc tgttcacccg cgagaaccag ttccagacct
     2701 acaacgcgat cctgaacggc acgatccccg cgctgacgtc gacgcgcgtc gacataccgt
     2761 ccgccgtgtc gcgcgccgtg ctcggcgggc tggcgcgcac gctcggcgag cgccatgcgt
     2821 cgtgccacgt gttcgccgag gcgctcaagg cggcggcggg cgcgggcggg atcgcgtcgc
     2881 gcatgacgat caagtcgacg atggagcgcc tgttcagcac cgagatcgaa tcgcagcgcc
     2941 agatgttgaa cgacgcgctg ccggaactac cgatgtcgga gctcgcggtc gccaggcagc
     3001 ctcgcaaggg cgagatcagc gagctgtccg gcctcttgct cgatgatctc ggcgatgtcg
     3061 gcgcgacgct gctcgacgat ctcgcgccga tcgatctcgc gctgtccagc gacgagaagg
     3121 cgaccgcgct cgaggacaag acctcgccgg acgacaagca accgaagccg gtgcccggca
     3181 gcatcgcgcg cgcgaagacg cccgttcccg ttccggaagt gacgccggtc gccagcgcgc
     3241 gtcaggcggt ccggaccgtg gcgctcaccg cgccgccgcc cgaagctccg ccgtcgtcgt
     3301 tcgggctctg gctcgtgatc agcgccggcg cggtcgtgct gagcgcgatc ctgtttgtcg
     3361 tcgtctgggt ctgggtctcc ggccaccgcg tcgagatcga ggtcctcgct ccgagccggg
     3421 cgccggcgat gatccagaac gccgagccaa cgcgcgagga gccgtcgccg gcggcgctcg
     3481 acgccgcggt cgatgccggc gcggcgatcg accggccctg atgccgctgg cgatgccgac
     3541 gtcgagctgt ggtgtacacg ctcaccggca gccacagctg gtcctggttg gacggcggac
     3601 cttcgtgcgc gttatgctcg aaagccgttc ggcgctaaca tcgaacgggg agtcgttcca
     3661 tggcatctcg cggctggggc ctcgttccca agaccatctt gcccacgatc gtcgtctcgg
     3721 tggtgatcgc gagcgcggta ggcatcgtgc tctacctcga cgagaaagcg tcggtgatcg
     3781 gggaatcgcg caagaccgcg atcgcgatcg cgcagcagac actggccgag cgccaagtgt
     3841 acaccgcgaa cgtcgtcaac ccgctgctgc gcgacttcgg cggcccggat caagggttca
     3901 ccaccgggaa caacaccatc cactgggtca cgagcaagat gcacaatccg aaggacaagg
     3961 gttcgctgcc cttgccggcg gcgctcgtgc acctcgtgtc ggcgagcgtg aactcgcgcg
     4021 gcgccaaggg ccatacgctc gacctgctca gcctcgacgc ggtgaacccc gagaagaacc
     4081 cgctcaagac cggcggtagc ccgaccgaga tctccgcgat gcaattcctg cagtccgaga
     4141 agaacccgct gacgtaccgc gaagcggtca gcggcgaggg cgtcgacgca cgctacgtgc
     4201 tgtggtaccc cgacatcgca gccgatgcgt gcgtgagctg ccacaacggc ctcggcggcg
     4261 ggcacaagaa cgactgggtc aggggcgatg tgatgggctc gatcaaggta acgttgccga
     4321 tgggcaccga cctcgagaat gccaagagcg gtgcgtttcg tatcatgctg ctggtgctgg
     4381 gcgcgttcgc ggtcgtcgcg ctgctccaga cgctcttgca atggctgttc gtgagccggc
     4441 cgctgattcg caacctcaac atgctcgagc gcgcggccga gcgcatctcg catggcgagg
     4501 tcgaccagcc gatcaagatc gagagcaacg acgaggtcgg ccggctcggc cagtcgttcg
     4561 agcggatgcg cacgagcctg gtgaccctgc tcgacgaggc c
//
LOCUS       DASVOR010002133         6871 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_490559,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002133 DASVOR010000000
VERSION     DASVOR010002133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6871
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490559"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..515)
                     /locus_tag="VF516_39620"
     CDS             complement(<1..515)
                     /locus_tag="VF516_39620"
                     /inference="COORDINATES: protein motif:HMM:NF022698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 family protein"
                     /protein_id="HEX8113927.1"
                     /translation="MAGYPGKPLPKKLGIKAGHKVCLLNAPDSFARHLEQNDVRITHD
                     LRLPPVDVVVLFVDRVADLERRVGDIVARLHPRGGFWVAFRTSGRGSDITEDVVCRIA
                     LAAGMVQNKICEIDGSWSGVRLVLRDEIRDAISYREVPPPLSRRVRRPTAAARLARLS
                     TSGAGSTLRRAR"
     gene            1017..2066
                     /gene="hemH"
                     /locus_tag="VF516_39625"
     CDS             1017..2066
                     /gene="hemH"
                     /locus_tag="VF516_39625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEX8113928.1"
                     /translation="MPTGLLLVNLGTPDAPTPAAVRRYLREFLGDPRVIDIPALGRAL
                     LLNLIILPTRPAKSAHAYASIWDAERGSPLMFHSQDLAAAVAAQLGPAWHVELAMRYG
                     RPSLGVALHAFAEAGVERILVFPLFPQYATSSTGTAVARVMELAAERWNVPALDFVPA
                     FHDDPGFLTAFERIARPVLAELEPDHVLFSFHGLPVRQIRKSDPGDTGCLARETCCDE
                     GRNPYCYRAQSFATARALASRLALAADRYTVCFQSRLGRTPWIEPHTDQMIDRLARRG
                     VKKLAVMCPAFVADCLETLEEIGIRAREQFQAAGGERLVLVPSLNATAPWVDAVCAIA
                     TRHATPRLLPVVSGA"
     gene            2063..3205
                     /locus_tag="VF516_39630"
     CDS             2063..3205
                     /locus_tag="VF516_39630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113929.1"
                     /translation="MTALSDVYGNLLIVRPPVPATVNKAGANYKSEVAHLTYSPRRAY
                     DEWLSILDAIVACGGDALYQFEAADDPLLDHAALEVDGDGAIRPAGSREVVGRLDDVM
                     TGRVFAANGPWVTIAGGALRAVMPNMLEHRRAEAAYYRALLGELAAAAGLSLALADNP
                     HRWEGMADVAVVGDQVILTYAVPGHYDRDTTPKSSRSSRAGVAFAADSAGVPDDARIY
                     AELIYPHFHGDTVHFGARPTNRPPVLVHYRGGLYGDGAEVVAQRLGEDRIVAIAPHDA
                     VEQYAGNSRQVGSGVLVPDGVSPAFLADLQRLGLATHRVPLFELFGKAGGGPACATLY
                     LPRTLALPEQLPLRYSATRAAVHARRERIPETLQVNRSYFEGRARG"
     gene            3361..4794
                     /locus_tag="VF516_39635"
     CDS             3361..4794
                     /locus_tag="VF516_39635"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8113930.1"
                     /translation="MNRLLRPTIVAAWAAGALAALTSAASANPVEIGGTAGIHVFAHD
                     NELGVADVPSADSERNSALFGVRLGVFFTPMFGVEGEFGLIPSESRQTTFDVWNIVYR
                     AHVVAQFRAQNPDVKLIPFVLAGGGFMTIVDNGGSKNDPLIAKDTDADVYLGVGAKYR
                     VDNGWGLRLDLRLLFPPSSKDNGFTEDFEALISVYKELGRQAPKPAAPVDDDPDKDGI
                     RGAADKCPNEPEDKDGFQDEDGCPDLDNDNDGIPDAQDKCPNEPEDKDGFQDEDGCPD
                     PDNDGDGIPDAQDKCPLEPEDKDGFQDEDGCPDPDNDGDGVPDAQDKCPLEPETKNGY
                     QDDDGCPDEIPAKLKQFTGVIQGINFRVSDAALQPSSNPTLDKAVAVLKEFGDLKLEI
                     QGHTDDQPLKGGGKFADNQALSQARADTVKAYLVSKGIDEARLTAKGYGDTVPVESPK
                     DLKGDKLNAARAKNRRVEFKLVSALTQ"
     gene            4893..5177
                     /locus_tag="VF516_39640"
     CDS             4893..5177
                     /locus_tag="VF516_39640"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEX8113931.1"
                     /translation="MLADALLDVLVCPKSKQPLIYFPRGEDDREEADAFLLCPASRLR
                     YPIRDGVPVMLVDEATPVEATKVDALLARARQLGLRLTGQSSTDGGSRSA"
     gene            5269..6216
                     /locus_tag="VF516_39645"
     CDS             5269..6216
                     /locus_tag="VF516_39645"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEX8113932.1"
                     /translation="MLKSLSIFTGNSNRALADEICKFVEIPLGRADVTHFSDGEIYVE
                     VGENVRGVNCFVVQPTCSPPNQALMELLIMIDALKRASAGSIVAIIPYFGYARQDRKA
                     KPRTPITAKLVANLITAAGADRALAMDLHAGQIQGFFDIPFDHLYAMPVLIDELRVLG
                     FGGEHTVVVSPDAGGVERARAFSKRLGAGLAIIDKRRPAPNVSEIMNIVGDVRGKKAV
                     IVDDIIDTAGTLTNGAHAIINAGAASVSACATHAVFSGKAVQRIEDSPLQHVVVTNTI
                     PLSDLGAACTKVRVKSVGRLLGEAIKRIHHGDSISSLFV"
     gene            6256..>6871
                     /locus_tag="VF516_39650"
     CDS             6256..>6871
                     /locus_tag="VF516_39650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEX8113933.1"
                     /translation="MEVGKLTVSLRGRSGKGGSRKLRAQGKVPGVCYGSSVEGRIEPL
                     PITVDVKQLRGALDPVRKRNTVISLTIEGGAGPRLLHALVKDVQIDALRHDITHVDLL
                     AIDPNQEVRAEVPLEFTGKPKGAIDGGQIRIVLRTLTVRAKPTDIPVKLTVDVSPLEI
                     GDVIHVSAIALPAGVASVTGRDLAVVTCAAPEEEKAAEAAAPGTE"
BASE COUNT         1088 a         2496 c         2301 g          986 t
ORIGIN      
        1 cgggcgcgcc gcagcgtcga gcccgcaccg ctggtcgaca gccgcgccag gcgagcggcg
       61 gcggtgggcc ggcgcacgcg gcgagacagc ggcggcggca cctcacggta cgagatcgcg
      121 tcgcggatct cgtcgcggag caccaggcgc acaccggacc agctgccatc gatctcgcag
      181 atcttgttct gcaccatgcc ggcggcgagc gcgatccggc acaccacgtc ctcggtgatg
      241 tcggagccac gacccgaggt acggaacgcg acccagaagc caccccgtgg gtggaggcgt
      301 gccacgatgt ccccgaccct gcgttccagg tccgccaccc ggtccacgaa caggaccacg
      361 acatcgaccg gtggcaggcg caggtcgtgg gtgatgcgga cgtcgttctg ctccaggtgg
      421 cgtgcgaagc tgtccggggc gttcagcagg cagaccttgt ggcccgcctt gatccccagc
      481 ttcttgggca gtggctttcc cgggtacccg gccatgacct tgggataatg cagcccccgc
      541 ccaggaggtc aaacaggaag cccaaatcat cttataacaa catgtaatta ttaaactaag
      601 tgctaagttg cgtgagctcg gtttttggtg gcttggacaa ccattcaccc cacccgatcc
      661 ggctcgttcc ggggccttgt cgaaaacccg ccaggggccc cggtcccgac cttgactttc
      721 gcgcctcccg ttcccggagc cccctttggg ggccctctgg gtcccccctc cttggacccg
      781 gtcgaaccac cgctgaacca ccgccagcgc ttcctgacgc gcgccagacg gtgtgcccgg
      841 cgagggaggt ccgcgcggcg aggccgacgt cgacccagct tgcgcccgcg cacccgacct
      901 cgaaccgacc gaccgctcga gcgcgcgccc acgcccgcgg ttttgcgctc gcccagggtt
      961 ctcggccgga gcaaaacatc gtactggtcg aacctcgagc cccccgcgag cgcgccatgc
     1021 ccaccggcct cctcctcgtc aacctcggca ccccggatgc gcccacgccc gcggcggtcc
     1081 gccgctacct gcgcgagttc ctcggcgatc ctcgcgtgat cgacatcccc gcgctcgggc
     1141 gcgcgctgct gctcaacctc atcatcctgc cgacccgccc cgcgaagtcg gcgcacgcgt
     1201 atgcgtcgat ctgggatgcc gagcgtgggt cgcccctgat gttccactcg caggacctcg
     1261 cggccgcggt cgccgcgcag ctcggcccgg cgtggcacgt cgagctggcg atgcgctacg
     1321 ggcggccgtc gctcggggtc gcgctccatg cgttcgccga ggccggcgtc gagcgcatcc
     1381 tggtgttccc gctgttccca cagtacgcga cctcgtcgac cggcaccgcc gtggcgcgcg
     1441 tgatggagct cgccgccgag cgctggaacg tgccggcgct cgacttcgtg ccggcgttcc
     1501 acgacgaccc cgggttcctc acggcgttcg aacggatcgc gcgcccggtg ctcgccgagc
     1561 tcgagcccga ccacgtgctg ttctcgttcc acggtctgcc ggtccgccag atccgcaagt
     1621 ccgaccccgg cgacaccggg tgcctcgcgc gcgagacctg ctgcgacgag ggcaggaatc
     1681 cctactgcta ccgcgcccag tcgttcgcga ccgcgcgtgc gctggcgtca cggctcgcgc
     1741 tcgccgcgga tcggtacacg gtgtgcttcc agtcgcggct gggacggacg ccgtggatcg
     1801 agccgcacac cgatcagatg atcgatcggc tcgcgcgccg cggggtgaag aagctggcgg
     1861 tgatgtgccc ggcgttcgtc gccgactgcc tggagaccct ggaggagatc gggatccggg
     1921 cgcgcgagca gttccaggcc gccggcggcg agcggctcgt gctggtgccg tcgctcaacg
     1981 ccaccgcacc gtgggtcgat gcggtgtgcg cgatcgccac ccgccacgcc acgccccggc
     2041 tgcttccggt agtctccggg gcatgaccgc gctgtccgac gtctacggca acctcctgat
     2101 cgtgcgcccg ccggtcccgg cgacggtcaa caaggcgggc gccaactaca agagcgaggt
     2161 cgcccacctc acctactcgc cgcgccgcgc ctacgacgag tggctgtcga tcctcgacgc
     2221 gatcgtcgcc tgcggcgggg acgcgctcta ccagttcgag gccgccgacg acccgctgct
     2281 cgatcacgcc gcgctcgagg tcgacggcga cggcgcgatc cgcccggccg gctcgcgcga
     2341 ggtcgtcggc cggctcgacg acgtgatgac cggccgcgtg ttcgccgcca acggcccgtg
     2401 ggtgacgatc gccggcggcg cgctgcgcgc cgtgatgccg aacatgctcg agcatcgccg
     2461 cgccgaggcc gcctactacc gcgcgctgct cggcgagctc gccgccgccg cggggctgtc
     2521 gctggcgctc gccgacaacc cgcaccgctg ggagggcatg gccgacgtcg cggtggtcgg
     2581 cgaccaggtg atcctgacct acgcggtccc cgggcactac gaccgcgaca ccacgccgaa
     2641 gagctcgcgc agctcgcgcg ccggcgtcgc gttcgcggcc gactccgcgg gcgtgcccga
     2701 cgacgcgcgg atctacgccg agctgatcta cccgcacttc cacggcgaca ccgtgcactt
     2761 cggcgcgcgg ccgacgaacc gcccgccggt gctggtccac taccgcggcg ggctgtacgg
     2821 cgatggcgcc gaggtcgtcg cacagcgcct gggcgaggac cggatcgtcg cgatcgcgcc
     2881 gcacgatgcc gtcgagcagt acgccggcaa ctcgcggcag gtcggcagcg gcgtgctcgt
     2941 gccggacggc gtgtcgccgg cgttcctcgc cgacctgcag cggctcggcc tggcgacgca
     3001 ccgcgtcccg ctgttcgaac tgtttggaaa agcgggcgga ggcccggcgt gcgccaccct
     3061 ctacctgccc cggacgctcg cgctgcccga gcagctgccg ctgcgctact ccgcgacccg
     3121 cgccgccgtg cacgcgcgcc gagagcgaat tccagaaaca ttacaagtga atagatcgta
     3181 tttcgagggt cgggcgcggg gctgaccgcc tccgtcggcg gcaacttaag ttgccgcacc
     3241 tgctggcatc cgatcgcgca ggcgagataa gaacgcggac gacatcctcc ggtcgccctc
     3301 gggattcctt ggggggcggc cgagcaggaa aggcccggac agccaaagga acgtcgcgcc
     3361 atgaatcgct tgcttcgacc taccatcgtt gccgcatggg ccgcgggtgc gctcgcagcc
     3421 ctgacgagcg cagcgagcgc caaccctgtc gagatcggcg gaaccgcggg gatccacgtc
     3481 ttcgcccacg acaacgagct gggcgtggcc gacgtcccca gcgccgattc cgagcgtaac
     3541 tccgcgctgt tcggggttcg cctcggcgtg ttcttcacgc cgatgttcgg ggtggagggc
     3601 gagttcgggt tgatcccgag cgagtcgcgc cagaccacgt tcgacgtgtg gaacatcgtc
     3661 taccgcgcgc acgtcgtcgc gcagttccgc gcccagaacc cggacgtcaa gctgatcccg
     3721 ttcgtgctcg ccggcggcgg cttcatgacg atcgtcgaca acgggggcag caagaacgac
     3781 ccgctgatcg ccaaggacac cgacgcggac gtctacctcg gcgtcggcgc caagtaccgg
     3841 gtcgacaacg gctggggcct gcgcctcgac ctccggctgc tgttcccgcc gagcagcaag
     3901 gacaacggct tcaccgagga cttcgaggcg ctgatctcgg tctacaagga gctcggacgc
     3961 caggcgccca agccggcggc gccggtcgac gacgatcccg acaaggacgg gatccgcggc
     4021 gccgccgaca agtgcccgaa cgagcccgag gacaaggacg gcttccagga cgaggacggc
     4081 tgccccgatc tcgacaacga caacgacggc atccccgacg cccaggacaa gtgcccgaac
     4141 gagcccgagg acaaggacgg cttccaggac gaggacggct gccccgatcc cgacaacgac
     4201 ggcgacggca tccccgacgc ccaggacaag tgcccgctcg agcccgagga caaggacggc
     4261 ttccaggacg aggatggctg ccccgatccc gacaacgacg gcgacggcgt gcccgacgcc
     4321 caggacaagt gcccgctcga gcccgagacc aagaacggct accaggacga cgacggttgc
     4381 ccggacgaga tccccgccaa gctcaagcag ttcaccgggg tgatccaggg catcaacttc
     4441 cgggtcagcg acgcggccct gcagccgagc tcgaacccga cgctcgacaa ggcggtcgcg
     4501 gtgctcaagg agttcggcga cctcaagctg gagatccagg gccacaccga cgatcagccg
     4561 ctcaagggcg gcggcaagtt cgccgacaac caggcgctgt cgcaggcgcg cgccgacacg
     4621 gtcaaggcgt acctggtcag caagggcatc gacgaggcgc ggctcaccgc caagggctac
     4681 ggcgacacgg tcccggtcga gagccccaag gacctcaagg gcgacaagct gaacgccgca
     4741 cgggccaaga accgccgtgt cgagttcaag ctggtcagcg cgctgaccca gtaggccgcg
     4801 ggccagggcc ccttcgtggg cccttccgta cgcccctccg tacagtggta gggaagtgtg
     4861 tctcggcggc cggcgcgtga tacgaagtac ccgtgctggc cgatgcgctc ctcgacgtcc
     4921 tggtctgtcc caagtcgaag cagccgctga tctacttccc gcgcggcgag gacgatcgcg
     4981 aggaagccga tgcgttcctg ctctgcccgg cgtcgcggct gcgctacccg atccgcgacg
     5041 gcgtcccggt gatgctggtc gacgaggcga cccctgtcga ggcgaccaag gtcgacgccc
     5101 tgctcgcccg cgcccgccag ctcggactgc gcctcaccgg gcagtcctcg accgacggcg
     5161 gttcgcgatc tgcgtgatcc tgcgatgtta gcgtcggcga agtgattctc gactcttgac
     5221 gcgacgtgcc cgcgactgct agatcgccgt cctacccggt gcatcgcggt gttgaagtcg
     5281 ttatcgatct tcacgggcaa ctcgaatcgc gcgctcgcag atgagatctg caagttcgtc
     5341 gagatcccgc tcggccgggc cgacgtcacc catttctcgg acggcgagat ctacgtcgag
     5401 gtgggtgaga acgtgcgcgg cgtcaactgc ttcgtcgtcc agccgacctg ctcgccgccc
     5461 aaccaggcgc tgatggagct gctgatcatg atcgacgcgc tcaagcgcgc gtcggccggc
     5521 agcatcgtcg cgatcatccc gtacttcggg tacgcgcggc aggaccgcaa ggccaagccg
     5581 cggacgccga tcaccgcgaa gctggtcgcc aacctgatca ccgcggcggg ggccgaccgc
     5641 gcgctcgcga tggacctcca cgccggccag atccagggct tcttcgacat cccgttcgat
     5701 cacctgtacg cgatgccggt cctgatcgac gagctccgcg tgctcgggtt cggcggcgag
     5761 cacaccgtgg tggtgtcgcc cgacgcgggc ggcgtcgagc gcgcgcgcgc gttctccaag
     5821 cggctcggcg ccggcctggc gatcatcgac aagcggcggc cggcgcccaa cgtctccgag
     5881 atcatgaaca tcgtcggcga cgtccgcggc aagaaggcgg tgatcgtcga cgacatcatc
     5941 gacaccgcgg gcacgctgac caacggcgcc cacgcgatca tcaacgccgg ggccgcgagc
     6001 gtcagcgcgt gcgcgaccca cgcggtgttc agcggcaagg cggtgcagcg gatcgaggac
     6061 tcgccgctgc agcacgtcgt ggtcaccaac accatcccgc tcagcgacct cggcgcggcg
     6121 tgcaccaagg tccgggtcaa gagcgtcggc cgcctgctcg gcgaggcgat caagcgcatc
     6181 caccacggcg attcgatcag cagtttgttc gtctaggctc ccaagatatt gaagatgggt
     6241 cgattggaga acgtcatgga agtcggaaag ctcacagtct cgcttcgcgg ccgctcgggc
     6301 aagggtggtt cgcgcaagct ccgcgcgcag ggcaaggtcc cgggcgtctg ctacgggtct
     6361 tcggtggagg gccgcatcga gccgcttccg atcaccgtcg acgtcaagca gctgcgcggc
     6421 gcgctcgatc cggtccgcaa gcgcaacacg gtgataagcc tcacgatcga aggcggcgcc
     6481 gggccgcgct tgctgcacgc gctggtcaag gacgtgcaga tcgatgcgct gcgccacgac
     6541 atcacccacg tcgacctgct cgcgatcgac cccaaccagg aagtccgcgc cgaggtgccg
     6601 ctggagttca ccggcaagcc caagggcgcg atcgacggcg gccagatccg catcgtgctg
     6661 cgcaccctga ccgtgcgcgc caagcccacc gacatcccgg tcaagctcac ggtcgacgtc
     6721 tcgccgctgg agatcggcga cgtgatccac gtcagcgcga tcgcgctgcc ggccggcgtc
     6781 gccagcgtga ccggccgcga tctcgcggtc gtgacctgcg cggctcccga ggaggagaag
     6841 gccgccgagg ccgctgcccc tgggaccgag g
//
LOCUS       DASVOR010002134         3747 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_257149,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002134 DASVOR010000000
VERSION     DASVOR010002134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3747
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257149"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1311
                     /locus_tag="VF516_39655"
     CDS             <1..1311
                     /locus_tag="VF516_39655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113934.1"
                     /translation="TWCLAAIERLAVLAISNAPLIVLVAWLGASLAIAPQWIYLVLAP
                     AVVWLARLWFASWVECLAARLDRRIVEGSASHDNPWSREIGDYLMGYVRRTGWDLDPR
                     LLAEVVFLPGKRIDGVVSYGGGATHVRIVVDRELLVMAMGPLLDDKADEKPALWPDWT
                     AACIAPIPGGRSPRAEPSPHDSRGRKPKTSYPGAQRKPLGQAATLLGYVTPTPGQLVP
                     LISDSPQDLAIVRELLSEHYPWFAPDPDEEYDATDPTDKDLLFGALAHELGAVRRNET
                     QLATLRLALGRRVARLTSRARARIADSYAALNFARHHLIQYLYYSWTRTTDALTARAR
                     PARLHEVSTRILAEVGDSHANPPRAIRAWLTWLSQFFPEPIVDRRRVLASRMAVGVVI
                     AALLVTAGIAVKRSIGYHPIYVERMNAQERDLKKGPVDGEAKPK"
     gene            1289..2113
                     /locus_tag="VF516_39660"
     CDS             1289..2113
                     /locus_tag="VF516_39660"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8113935.1"
                     /translation="MAKQNPNKADDSSLFMPMVSKATLAINKSSAGAGVLGLVGFAAL
                     FLVACVFFVTENYFVAMLIMSFALLVYVTGSELAKLFLTSFTIFFSSKHVIPGAAYLQ
                     ETLVPLSRTLMIRRDGEGNLKAGPLVKGMTIKLPDNPLARDIQTLLEKNPNFEYAEYI
                     TYQYYVQCHELYDHSSSHLEFVATAMPMFGLIATIIGLIGMFEGLGAEVTVEFLSPQL
                     ALALKCTLYGVLLAALYKIIASRFDQRLKALDYDFETFCRALQVVIDNKAAVEVRP"
     gene            2110..2706
                     /locus_tag="VF516_39665"
     CDS             2110..2706
                     /locus_tag="VF516_39665"
                     /inference="COORDINATES: protein motif:HMM:NF025057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HEX8113936.1"
                     /translation="MKYAVRRQKPKFDIWPVVYIDLMTQIMMFFVILWSISQMQAKRS
                     GASRTLGDTTLHEVTLPGDVLFPSGKTKLTPQGEEVFTKLFKDDTGEVLSFETGGLTR
                     RVLVIHGHTDSDGKKDDNYVLGFQRALTVYKEIQKYGPEITDHAVLCTHADNTPAKEV
                     PLFTGTLTPAQAQAISEAKTKNRRITIEDQVTSAVKQE"
     gene            2703..>3747
                     /locus_tag="VF516_39670"
     CDS             2703..>3747
                     /locus_tag="VF516_39670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113937.1"
                     /translation="MNPPRHRSEGFGHVAGALGARSVTAVGLLAGTLVTMWRMERFRP
                     DYWAQLSPWVMFLAGGFAVLAGTLVFVYWRLAERSHSYLRFGEKLPFGDETALLGARG
                     RSLVVGRAPFVDVVDLPPRATHALYVAIMLGIALIGLDNRAVALLREVPSRFDRSSID
                     YCKPPEPPKRETKHSQGCKLVERAYQLGYTRSLGSCAPAEAKREVSQVCTRRQLDEPQ
                     LHYAWRRLADRIDDLRTTDDGPGSLDRFEHQLDHLPAIVHGTLDTVAMRARSSHHLFT
                     NLPDPRPGLRDRVDGLLERGCGARLAHLPHFPRMTDGPTGPSKLFEHVLAQLMFNPSY
                     KPIIAQCEEIVVHW"
BASE COUNT          604 a         1307 c         1231 g          605 t
ORIGIN      
        1 acctggtgtc tcgccgcgat cgagcggctc gccgtgctcg cgatcagcaa cgcaccgctg
       61 atcgtgctcg tcgcgtggct cggggcgtcg ctcgcgatcg cgccgcagtg gatctacctg
      121 gtgctggcgc cggcggtcgt atggctcgcg cggctgtggt tcgcgtcgtg ggtcgagtgc
      181 ctcgcggcgc ggctcgatcg ccggatcgtc gagggctcgg cgtcgcacga caacccgtgg
      241 tcgcgcgaga tcggcgacta tctgatgggg tacgtgcggc gcaccggctg ggatctcgat
      301 ccccggttgc tcgcggaggt ggtgttcctg cccggcaagc ggatcgacgg cgtcgtctcg
      361 tacggcggcg gcgcgaccca cgtgcgcatc gtggtcgacc gcgagctgct cgtgatggcg
      421 atgggcccgc tgctcgacga caaggccgac gagaaaccgg cgctgtggcc cgactggacg
      481 gccgcctgca tcgcgccgat tcccggcggc agatcgccga gagccgagcc atcaccccac
      541 gactcccgcg gccgcaagcc gaagacgagc tatccgggag cccagcgcaa gccgctcggt
      601 caggccgcga cgttgctcgg ctacgtgacc cccacgccgg gccagctcgt gccgctgatc
      661 tcggacagtc cacaggacct cgcgatcgtg cgcgagctcc tgtcggagca ttatccctgg
      721 ttcgcgcccg atcccgacga ggagtacgac gcgaccgatc cgacggacaa ggacctgctg
      781 ttcggcgcgc tcgcccacga gctcggcgcg gtgcgccgca acgagaccca gctcgcgacc
      841 ctccggctgg cgctcggccg ccgcgtcgct cgcctgacgt cgcgcgctcg cgcacggatc
      901 gccgatagct acgccgcgct caacttcgcg cgccaccacc tgatccagta tctctactac
      961 tcctggacca ggaccaccga cgcgctgacg gcgcgtgccc gcccggcgcg cttgcacgag
     1021 gtctcgaccc gcatcctggc cgaggtcggc gattcccatg ccaacccacc gcgcgcgatc
     1081 cgcgcctggc tcacgtggct atcgcagttc ttccccgaac cgatcgtcga tcggcggcgc
     1141 gtgctcgcga gccggatggc ggtgggagtc gtgatcgccg cgctgctcgt cacggccggg
     1201 atcgcggtga agcgctcgat cggataccac ccgatctacg tcgagcggat gaacgcgcaa
     1261 gagcgcgatc tcaagaaagg cccagtcgat ggcgaagcaa aacccaaata aggcggatga
     1321 ctcctcgctg ttcatgccga tggtcagcaa ggcgaccctc gcgatcaaca agtcctccgc
     1381 cggcgccggc gtgctcgggc tggttggctt cgcggcgctg ttcctcgtcg cctgcgtgtt
     1441 cttcgtgacc gagaactact tcgtcgcgat gctgatcatg tcgttcgcgc tgctggtcta
     1501 cgtgaccggc agcgagctcg cgaagctgtt cctgacctcg ttcacgatct tcttctcgag
     1561 caagcacgtg atcccgggcg ccgcgtacct gcaggagacg ctcgtgccgc tgagcaggac
     1621 gctgatgatc cggcgcgacg gcgagggcaa cctcaaggcc gggccgctcg tcaagggcat
     1681 gacgatcaag ctgcccgaca acccgcttgc ccgtgacatc cagacgctgc tcgagaagaa
     1741 ccccaacttc gagtacgccg agtacatcac gtaccagtac tacgtgcagt gccacgagct
     1801 gtacgaccac tcgagctcac acctcgagtt cgtggcgacc gcgatgccga tgttcggctt
     1861 gatcgcgacg atcatcggcc tgatcggcat gttcgagggc ctcggcgccg aggtcacggt
     1921 cgagttcctg tcgccgcagc tcgcgctcgc gctcaagtgc accctgtacg gcgtcctcct
     1981 cgcggcgctc tacaagatca tcgcgtcgcg cttcgatcag cggctcaagg cgctcgacta
     2041 cgacttcgag acgttctgcc gcgcgctgca ggtggtgatc gacaacaagg cggctgtcga
     2101 ggtgcgaccg tgaagtacgc cgtgcgccgc cagaagccga agttcgacat ctggccggtc
     2161 gtctacatcg atctgatgac acagatcatg atgttcttcg tgatcctgtg gtcgatcagc
     2221 cagatgcagg cgaagaggag cggcgccagc aggacgctcg gcgataccac gctgcacgag
     2281 gtgacgttgc ccggtgacgt gctgtttccg tccggaaaga ccaagctgac gccgcagggc
     2341 gaggaggtgt tcacgaagct gttcaaggat gacaccggcg aggtgctgtc gttcgagacc
     2401 ggcgggctca cccggcgcgt gctcgtgatc cacggccaca ccgacagcga cggcaagaag
     2461 gacgacaatt acgtgctcgg gttccagcgg gcactcaccg tctacaagga gatccagaag
     2521 tacggtccgg agatcaccga ccacgcggtg ctgtgcacgc acgccgacaa cacgcccgcc
     2581 aaggaagtcc cgctgttcac cggtacgctc acgcccgcgc aggcgcaggc gatctccgaa
     2641 gcgaagacca agaaccggcg catcacgatc gaggaccagg tcaccagcgc ggtgaagcaa
     2701 gaatgaaccc gccgcgtcac agatccgagg ggttcggcca tgtcgccgga gccctcggtg
     2761 cacgctcggt cacggcggtc gggctgctcg ccggcaccct ggtcacgatg tggcgcatgg
     2821 agcggtttcg cccggactac tgggcccagc tgagcccgtg ggtgatgttc ctcgccggcg
     2881 ggttcgcggt gctcgccggc accctcgtgt tcgtgtactg gaggctcgcc gagcgcagtc
     2941 actcctacct gcgatttggc gagaagctgc cgttcggcga cgagaccgcg ctgctcggtg
     3001 cacgcgggcg ctcgctcgtc gtcggccggg ccccgttcgt cgatgtcgtc gaccttccgc
     3061 cgcgcgcaac gcacgccctg tacgtggcga tcatgctcgg catcgcgctg atcggcctgg
     3121 acaaccgcgc ggtcgccctc ctgcgcgagg tgccgtcgcg gttcgaccga tcgagcatcg
     3181 actactgcaa gccgccggag ccgccgaagc gcgagacgaa gcacagccag ggctgcaagc
     3241 tcgtcgagcg cgcgtaccag ctcggctaca cccggagcct cggctcgtgc gcgcccgccg
     3301 aggcgaagcg cgaggtgagc caggtgtgca cgcggcgcca gctcgacgag ccgcagctgc
     3361 attacgcgtg gcgccggctc gccgatcgga tcgacgatct gcgcacgacc gacgatggcc
     3421 ccggcagcct ggaccgcttc gagcaccagc tcgaccacct cccggcgatc gtgcacggga
     3481 cgctcgacac cgtggccatg cgcgcgcgct cgtcccatca cctgttcacg aacctgcccg
     3541 atccgcggcc ggggctccgc gatcgcgtcg atgggctgct cgagcgcggc tgcggcgcgc
     3601 gcctcgcgca tctgccgcat ttcccgcgca tgaccgacgg tccgaccggc ccgagcaagc
     3661 tgttcgagca cgtgctcgcc cagctgatgt tcaacccgag ctacaagccg atcatcgcgc
     3721 agtgcgagga gatcgtcgtg cactggg
//
LOCUS       DASVOR010002135         2072 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_111443,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002135 DASVOR010000000
VERSION     DASVOR010002135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111443"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VF516_39675"
     CDS             <1..246
                     /locus_tag="VF516_39675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113938.1"
                     /translation="PAAAPAPRPAAAPAPRPAAPRSAPGHRLVLDAGGRKVETTLYLD
                     GNTLRMDSQETHASPEAASKALERLKKLLAGEGYQAG"
     gene            complement(766..1416)
                     /locus_tag="VF516_39680"
     CDS             complement(766..1416)
                     /locus_tag="VF516_39680"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEX8113939.1"
                     /translation="MDNPAALASLPIFPLPNCVLLPGGLLPLLVFEPRYRELTRDCLA
                     GHQLMGIARLRPGFESAYYGRPPVYEKCGVGRILCSEELADGRFRLLLRGVARAEIAR
                     ELPADRMYRLVEARALDDASYDPVDAHDHLHRLIKLCDRLAEVIPKGGSELRDLVRAF
                     DTPGACADAVAAALVMDADARQELLEASDPMVRLQRTLGHVSHLLCELAPCQGAVN"
     gene            complement(1456..>2072)
                     /locus_tag="VF516_39685"
     CDS             complement(1456..>2072)
                     /locus_tag="VF516_39685"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8113940.1"
                     /translation="AGAEVALWARDAAALQRVADEVAARGGRALPMVCDVTDSTAVNS
                     TAEAVRRAMAPVTVVVNNAGAVLRKPTLAMSDIEWRRMIAVNLDGTFHVTVAFLPDLI
                     RAGGRIINISSIAGRQGTAMLAAYCAAKHAVVGFTRALAEELRTAKVSVNAICPGSVD
                     TVMLREGMPGASPDMSPDDIARTALFLAHTAPPALTGACIDVFG"
BASE COUNT          333 a          733 c          699 g          307 t
ORIGIN      
        1 cccgccgccg ctccggcccc caggcccgcc gctgctcctg ctcccaggcc agcagcgccg
       61 cgcagcgcac ccggccaccg cctcgtgctc gacgccggcg ggcgcaaggt cgagaccacg
      121 ctgtacctcg acggcaatac cctgcggatg gactcccagg agacccatgc cagccccgag
      181 gcggcgagca aggccctcga gcgcctgaag aagctgctcg ccggcgaggg ctaccaggcc
      241 ggctgaagat ggacgtcgac ctggcccttg acatggacgg ccggcgtgga gcttggcgtg
      301 gccgtctgat gattcatcac aggttgcgcg ttcgtggccg tcgccgtggc gctcgacgtc
      361 gacatcgacg atgacatcga cgatgacgtc gacgatgacg tcgaccttga ctccagcatt
      421 gagcttgacg gcagcgaagc gatacgtaga cgtctgaccc caccagtgac ctcgacctcg
      481 cccctgttgt cgcgctccgt cgaggctcgc agcacaggat cggtcgacgt ccaacgccga
      541 tggtgggtca acgtcgtacg tcgccgtcaa cctcaacgct ctgggtcaac gatcaggtcg
      601 acgtcgacga tcacggacgc catgtcgaaa cgtcttcgca aggacgttcc cggccgttca
      661 tcgacaccgc ttcctttcaa tcgagtcgcg ggatcaccgg ccgagcagcg gatcggtcat
      721 gctgtcgttg ccggtctcga ggtggccggc gcgccgccga tcgggtcagt tgaccgcgcc
      781 ctggcagggc gcgagctcgc acagcagatg gctgacgtgg ccgagcgtgc gctgcagccg
      841 gaccatcgga tcgctggcct cgagcagctc ctggcgcgcg tcggcgtcca tcaccagcgc
      901 ggcggcgacc gcgtcggcgc acgcgcccgg cgtgtcgaac gcgcggacca ggtcgcgcag
      961 ctcgctgccg cccttgggga tcacctcggc gagccggtcg cacagcttga tcagccggtg
     1021 caggtggtcg tgggcgtcga ccggatcgta cgacgcgtcg tcgagcgcgc gggcctcgac
     1081 cagccggtac atccgatcgg cgggcagctc gcgcgcgatc tcggcccgcg cgacgccgcg
     1141 gagcagcagc cggaaccgcc cgtcggccag ctcctcggag cacaggatcc ggcccactcc
     1201 gcatttctcg tacaccggcg ggcggccgta gtacgcgctc tcgaagccgg gccgcagccg
     1261 cgcgatgccc atcagctggt ggccggcgag gcagtcgcgg gtcagctcgc gatagcgcgg
     1321 ctcgaacacg agcagcggca gcagcccgcc ggggagcagc acgcagttgg ggagcgggaa
     1381 gatcggtaac gacgccagcg cggcgggatt gtccaatcca agttcgcgca tggtcgtcgc
     1441 atccctccgg tgttctcagc cgaacacgtc gatgcaggcg ccggtcaacg ccggcggggc
     1501 tgtgtgagcc aggaacagcg ccgtgcgagc aatgtcgtcc ggactcatat ccggactcgc
     1561 tcccggcatg ccttcacgca gcatgacggt gtcgacgctg ccaggacaga tagcattcac
     1621 ggagacctta gcagtgcgca gctcctccgc aagggcccgc gtgaaaccga cgaccgcatg
     1681 ctttgcggca caataggcgg cgagcatcgc cgtgccttgg cggcctgcga tcgacgagat
     1741 gttgatgatg cgaccgcccg cccggatcag gtccgggagg aatgctacgg tcacgtggaa
     1801 cgtaccatcg aggttcacgg cgatcatccg ccgccactcg atatcactca ttgccagggt
     1861 aggtttgcgc agcaccgcgc cggcgttgtt gacgaccacc gtgaccgggg ccatcgctcg
     1921 ccggaccgcc tccgccgtgc tattgaccgc cgtcgagtcg gtaacgtcgc acaccatcgg
     1981 cagggcgcga ccgccccgcg cggcgacctc gtctgcgacg cgctgcagcg cggcggcgtc
     2041 gcgggcccac agggcgacct cggcgccggc ct
//
LOCUS       DASVOR010002136         2270 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_227556,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002136 DASVOR010000000
VERSION     DASVOR010002136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227556"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VF516_39690"
     CDS             <1..302
                     /locus_tag="VF516_39690"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8113941.1"
                     /translation="PPANGPALLVLTGPRAGQRIPLQNGFTIGKAPSSSLMLDDGYTS
                     TNHAQIGMDASGACRLYDRNSTNGTFVNGVRVTEVVLDHGMSVRIGSTELRFLAQ"
     gene            314..>2270
                     /locus_tag="VF516_39695"
     CDS             314..>2270
                     /locus_tag="VF516_39695"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEX8113942.1"
                     /translation="MPQGNGELIIGGQIFKIDAPVVNWHKSGWDATSPYCIPTRTDPA
                     PRCLPAGTGQYPYGPPPLLYTQRYAVRPSLRSVPKSNNGMNPSYEAVKAVVKQFVVHH
                     DGCSSADMCFSVLQNERGLSVHFLLDNDGTIFQTIDLALMAYHASDWNLYSIGVELCN
                     RGDAFNVPSYYDGGRFGPRRDIKPVRINGNTLKAFDYTPAQKESFSQLCRELLRLFPN
                     LPAEYPQSSPGEPLWDTMRDAPIKRESYAGYVGHYHINTQKWDPGVFDFKEFCSKLRG
                     AFCFPVFPKLEPQAADKDKSKPIVPNDSGELKDAANALYALNEARADGGFFPVGPWGD
                     TALWHGGIHIVGKPGGGVFAPFPGRLVAASMGRESAIGSFNFLLLRHEMTLGSKKLQF
                     YSLYMHLADETRRDNPPEWMTKGGWKASAKRGQVTLLDEPIEAGALIGHVGTVGPDAL
                     SRPQVHVEFFSEQMIEDSNYWTLIDGTSTGRFCEAPEILGVIDSNHDNLLQRGELSQF
                     FASGGGGQFHPLVTLHVSEWTAEPNWAAALAASKGFKAMKVADIDAMVAEQITPGLWW
                     DAQVAAHCRLPKDGVVHHYHPVAFISWFKNQLIEAAAEAAKSGPKINEADIRAVPKSI
                     TDDLGDVTGASMRSSADVTEDPCNKNLT"
BASE COUNT          402 a          796 c          745 g          327 t
ORIGIN      
        1 ccccgccggc gaacggcccg gcgctgctgg tgctgaccgg cccgcgcgcc ggccagcgca
       61 tcccgctgca gaacggcttc acgatcggca aggcaccgag ctcgagcctg atgctcgacg
      121 acggctacac gtcgaccaac cacgcccaga tcggcatgga cgcgtccggc gcctgccggc
      181 tgtacgaccg caactcgacc aacggcacgt tcgtgaacgg cgtacgggtg accgaggtcg
      241 tgctcgatca cgggatgagc gtgcggatcg gctccaccga gctgaggttc ctggcacaat
      301 agggagtcga gcgatgccgc aagggaatgg agagctgatc atcggcgggc agatcttcaa
      361 gatcgatgcc ccggtggtga actggcacaa gagcggatgg gatgcgacga gcccgtactg
      421 catcccgacc aggaccgatc ccgcgcccag gtgcctgccg gcgggcaccg gccagtatcc
      481 gtacggcccg ccgccgctgc tgtacaccca gcgctacgcc gtccggccga gcctgcgctc
      541 ggtgccgaag tccaacaacg gcatgaaccc gagctacgag gcggtcaagg cggtggtcaa
      601 gcagttcgtc gtccaccacg acggctgcag ctcggccgac atgtgcttca gcgtgctgca
      661 gaacgagcgc gggctgtcgg tccacttcct gctcgacaac gacggcacga tcttccagac
      721 catcgatctg gcgctgatgg cctatcacgc gtcggactgg aacctgtact cgatcggcgt
      781 cgagctgtgc aatcgcggcg acgcgttcaa cgtgccgagc tactacgacg gcggccggtt
      841 cggtcccagg cgcgacatca agccggtccg gatcaacggc aacacgctca aggcgttcga
      901 ctacacgccg gcccagaagg agtcgttctc ccagctgtgc cgcgagctgc tccggctgtt
      961 ccccaacctg ccggccgagt atccgcagtc gagccccggc gagccgctgt gggacacgat
     1021 gagggacgcg ccgatcaagc gcgagagcta cgcgggctac gtcggccact accacatcaa
     1081 cacccagaag tgggatcccg gggtgttcga cttcaaggag ttctgcagca agctgcgcgg
     1141 cgcgttctgc ttcccggtgt tccccaagct cgagccccag gccgccgaca aggacaagtc
     1201 caagccgatc gtccccaacg acagcggcga gctcaaggac gccgccaacg cgctgtacgc
     1261 gctcaacgag gcccgcgccg acggcgggtt cttcccggtc ggcccgtggg gcgacaccgc
     1321 gctgtggcac ggcgggatcc acatcgtcgg caagcccggc ggcggcgtgt tcgcgccgtt
     1381 tcccggccgg ctggtcgcgg cgtccatggg ccgcgagtcg gcgatcggct cgttcaactt
     1441 cctcctgctc cgccacgaga tgacgctggg gtccaagaag ctccagttct actcgctgta
     1501 catgcacctc gccgacgaga ccaggcgcga caacccgccc gagtggatga ccaagggcgg
     1561 ctggaaggcg agcgcgaagc gcggccaggt caccctgctc gacgagccga tcgaggccgg
     1621 cgcgctgatc ggccacgtcg gcacggtcgg ccccgacgcg ctgtcgcgcc cccaggtcca
     1681 cgtcgagttc ttctccgagc agatgatcga ggactcgaac tactggacgc tgatcgacgg
     1741 cacgtcgacc gggcggttct gcgaggcgcc cgagatcctc ggcgtgatcg actcgaacca
     1801 cgacaacctg ctccagcgcg gcgagctgtc ccagttcttc gccagcggcg gcggtggcca
     1861 gttccacccg ctggtcacgc tgcacgtctc ggagtggacc gccgagccga actgggccgc
     1921 cgcgctcgcg gcgtcgaagg ggttcaaggc gatgaaggtc gccgacatcg atgcgatggt
     1981 cgccgagcag atcacacccg gcctgtggtg ggacgcccag gtcgcggcgc actgccggct
     2041 gcccaaggac ggcgtcgtcc accattacca cccggtcgcg ttcatcagct ggttcaagaa
     2101 ccagctgatc gaggcggcgg ccgaggccgc caagagcggc ccgaagatca acgaggcgga
     2161 catccgcgcg gtacccaagt cgatcaccga cgacctcggc gacgtcactg gcgcctcgat
     2221 gcggtcgtcc gccgacgtca ccgaggatcc gtgcaacaag aacctgaccc
//
LOCUS       DASVOR010002137         2510 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_549370,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002137 DASVOR010000000
VERSION     DASVOR010002137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549370"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF516_39700"
     CDS             <1..255
                     /locus_tag="VF516_39700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8113943.1"
                     /translation="VELGGIWTDEAARGAGVARAMVRALLARAEMPNRPLWCIPFAHL
                     AAFYESFGFAPVAPPWPSPIAAKVADCIAHALPDVVVLAR"
     gene            583..2037
                     /locus_tag="VF516_39705"
     CDS             583..2037
                     /locus_tag="VF516_39705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113944.1"
                     /translation="MERGTVLLGKYRVESVLGRGGMAVVLKVIHVHLEEELALKVLLP
                     EGALNDDVTARFLREAQSAVRLRGEHVARVSDVGVLPDGLPFMVMEYLRGIDLCGEIA
                     RRGSLPPGEAVDHVLQACEALAEAHALGIVHRDIKPANLFLTARPDGSPLIKVLDFGI
                     SKTPLHGAVPATRTEVVMGTPGYMSPEQMKASKDVDASTDIWALGVVLYECLSGRRPF
                     HGESFAATVLMAGTEPPPPLDPRIPLGLQAAVLRCLEKDRRERFRSVAALAAALAPFA
                     RNRREAETIVERTAHMQDGLPPGIEPALHWVPSAATTLSQSAGATAARPRRRLAVTRA
                     ASLGSLALLTVMALIVSVQLYPGEPPEMESLPRAGGQPAAEPVVEASGVGHSDDRAVP
                     APPAACDVLDAIVQEARGRDGAGDPRSALQLIQEVLRCPQGTSVMRYILAGRYACRAR
                     DHEAASQYRKQVPIELRREIEQACRKVGIHLLDP"
BASE COUNT          367 a          864 c          878 g          401 t
ORIGIN      
        1 gtcgagctcg gcgggatctg gaccgacgag gccgcgcgcg gcgccggcgt ggcccgcgcg
       61 atggtccgcg cgttgctcgc gcgcgccgag atgccgaacc gcccgctgtg gtgcatcccg
      121 ttcgcccacc tcgccgcgtt ctacgagtcg ttcggcttcg cgccggtggc gccgccgtgg
      181 ccgtcgccga tcgcggccaa ggtcgccgac tgcatcgcgc acgcgctgcc cgacgtggtc
      241 gtgctcgccc ggtaagcgcg gtgccagccg cggcgcatcg atgttcgccc ggcgctcacc
      301 ccccggcacg tttcggtttg acatcgatcg cgccggatcg cgtgacgcac gcctgacgat
      361 cgcattcgtg caacgtacat ggattctcgt gaacctggac gttgtcactg gtgacgtaat
      421 cggctcccgg acgatgtgga aatgcatgtg tccgcgctga cgtcgccaac actctcccgc
      481 gatctcttca cccggttcga acgcctgggt aagggcctcc ggacgaggtc acgcttgcaa
      541 gtcaacaggc ttcctctagt ctcggtatga atgggggaag gattggagcg gggcaccgtt
      601 ctgctgggga agtaccgcgt cgagagcgtg ctgggccgag gcgggatggc ggtcgtcctc
      661 aaggtcatcc acgtccatct cgaagaagag ctggcgttga aggtcctact gcccgaaggt
      721 gccctgaacg acgatgtcac ggcgcgattt ctgcgcgagg cgcagtccgc ggttcgtctg
      781 cgcggcgagc acgtcgcgcg ggtcagcgat gtcggcgtgt tgcccgatgg gcttcccttc
      841 atggtcatgg agtacctgcg aggcatcgat ctgtgcggcg agatcgcccg ccgagggtcc
      901 ctgcctcccg gtgaggcggt cgaccatgtg ctccaggcat gcgaggcgct ggccgaagcg
      961 cacgcactcg ggatcgtgca ccgcgacatc aagccggcaa acctgttctt gacggcgcgt
     1021 cccgacggct cgccgctcat caaggtcctc gatttcggga tctccaagac ccctctgcac
     1081 ggggccgttc cggccaccag gaccgaggtg gtgatgggga cgcccggcta catgtcgccc
     1141 gagcagatga aggcgagcaa ggacgtcgat gcgagcaccg atatctgggc actcggcgtg
     1201 gtgctctacg agtgcctgag cggtcgccgt ccgtttcacg gcgagtcgtt cgcggcgacg
     1261 gtcctgatgg ccgggacgga gccgccaccg ccgctggacc cgcgcattcc gctggggttg
     1321 caagccgccg tgctccggtg cctggagaaa gaccgtcgag agcgatttcg atcggtcgcc
     1381 gcgctcgccg ctgcgctggc tccgtttgcg cgcaaccggc gagaggccga gacgatcgtg
     1441 gagcgcacgg ctcacatgca ggatgggctg ccgcctggca tcgagccggc cttgcactgg
     1501 gtgccatcgg cggcaacgac actcagccag agcgccggcg cgacggcggc gagacccagg
     1561 cggcgtctcg cggtcacccg ggcagcctcg ctgggctcgc tggcgttgct gaccgtcatg
     1621 gcgctgatcg tctcggtgca gctctacccg ggggaaccac cggagatgga atcgctcccc
     1681 cgcgccggcg ggcaacccgc cgcggagccg gtcgtcgagg cgagcggcgt cggacacagc
     1741 gacgatcgcg ccgtgcctgc gccgcccgcg gcctgcgatg tgctcgatgc catcgtccag
     1801 gaggccaggg gacgcgatgg tgcaggggat ccccgatcgg cgctgcagct gatccaggaa
     1861 gttctccgct gcccgcaagg aacgagtgtc atgcgatata tcctcgccgg gcggtatgcc
     1921 tgtcgtgcgc gcgaccacga ggcggcgagc cagtatcgca agcaagtccc gatcgagctg
     1981 cgacgggaga tcgaacaggc atgccggaag gtggggatcc acctgctgga tccctgaccg
     2041 tgcccagggg cggaaccgcg gcgagccggc cggcgcgggg acgagctccg ggacgcgcgg
     2101 ggcccgcgcc gcccgccgtg atcaccgcgg tacacacgct cgctcaccgc gcgaggcaga
     2161 tcgaacgccg gcgctggcgc caactgtccg gaatcaggat gagctggcag tggcatggcg
     2221 gctgctccag gtccgctcag cccatgacga acatcgaact gaaccaactg gaaacgacca
     2281 ccggcgggtg tgcacccgtc ccctgctgtc ctcccccttg cgcgccgtgc ccgccgccgg
     2341 cccctgccgc cgcctggcgg tgggcggctc acgcccagta ccgcgcggcc gccgcctact
     2401 acggctacgg cggctacggc tacggctacg cacccgccgc ccgccgctgg tggtggtgag
     2461 ccggcagctc atcagattcc gcgcgtccct cgcttcgctg tttcggggtc
//
LOCUS       DASVOR010002138         1837 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_284887,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002138 DASVOR010000000
VERSION     DASVOR010002138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284887"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..959
                     /locus_tag="VF516_39710"
     CDS             <1..959
                     /locus_tag="VF516_39710"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEX8113945.1"
                     /translation="NRFAQSDATVLIQGETGAGKEGVADAIHQCSPRRDGPFVVLDCS
                     AIPEQLFEDQIFGHEQGAFTGAGRATIGVFEAAHGGTLFLDEIGELPLDVQSKLLRAV
                     ETRKVRRIGSTKVIASDVRIIAATNRDLATEVNRGTFRSDLFYRLSVAKLSVPALRER
                     REDIPLLVEHFLRQLVATHGDPRLPDDFLARAQRHAWPGNVRELRNAVERAVLLPNHP
                     TLGFEAPPRKDGQFGDVDIDVPFKVAKQKLVDEFDRRYLEALLEAHDNNISAAARAAG
                     IERMSIYKMIRRLGLDKTEGRDVKNGNGHDDDGDDESTDHQR"
     gene            1333..>1837
                     /locus_tag="VF516_39715"
     CDS             1333..>1837
                     /locus_tag="VF516_39715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113946.1"
                     /translation="MFERSVDPGGARPSAGFSLARVEYELQLHAALLRTMRAMLQTQR
                     VQREIDHPALPPILEELDALEHHIAELPHRADDRPMLALARRLGCGDHEIGFLWTAVA
                     LAVNPRVLVHARGLDDTASRGLSVSLYGRIANLEADASRALGLSLLPGHPTARHGLLV
                     PARGEWVP"
BASE COUNT          275 a          692 c          615 g          255 t
ORIGIN      
        1 tcaaccggtt cgcccagtcc gacgccaccg tcctgatcca gggcgagacc ggcgccggca
       61 aggagggcgt cgccgacgcg atccaccagt gctcgccgcg ccgcgacggc ccgttcgtcg
      121 tgctcgactg ctcggcgatc cccgagcagc tgttcgagga ccagatcttc ggccacgagc
      181 agggcgcgtt caccggcgcc ggccgcgcca cgatcggcgt gttcgaggcc gcccacggcg
      241 gcacgctgtt cctcgacgag atcggcgagc tgccgctcga cgtccagtcc aagctgctgc
      301 gcgcggtcga gacccgcaag gtccgccgga tcggctcgac caaggtcatc gcgtccgacg
      361 tccgcatcat cgccgccacc aaccgcgacc tcgccaccga ggtcaaccgc ggcaccttcc
      421 ggtccgacct gttctaccgg ctcagcgtcg ccaagctcag cgtccccgcg ctgcgcgagc
      481 gccgcgagga catcccgctg ctcgtcgagc acttcctgcg ccagctggtc gccacccacg
      541 gcgacccgcg gctgcccgac gacttcctcg cccgcgccca gcgccacgcc tggcccggca
      601 acgtccgcga gctgcgcaac gccgtcgagc gcgccgtcct gctccccaac cacccgacgc
      661 tcggcttcga ggccccgccg cgcaaggacg gccagttcgg cgacgtcgac atcgacgtcc
      721 cgttcaaggt cgccaagcag aagctggtcg acgagttcga ccgccgctac ctcgaggccc
      781 tgctcgaggc ccacgacaac aacatctccg ccgccgcccg cgccgccggc atcgagcgga
      841 tgtcgatcta caagatgatc cgccgcctcg gcctcgacaa gaccgagggc cgagatgtga
      901 agaacggcaa cggccacgac gacgacggcg acgacgagtc caccgaccac cagcggtgat
      961 gcgccgcggc ccgatctggc atcgagcaca cgtcgatcgc gagatcatcc gccactcggc
     1021 ctcgacttga ccgccgctca ccagccagcg ccggcaatgc gtgcctcacg cggccgtccg
     1081 tcgctccgat gtaggtggcg tacgcatggc gacactccgg cgggcgaagt ggccgcgatg
     1141 attgtcggtc gtgccgagca gtgtctcccg cggagacact gttcggcgag tttcggagcc
     1201 atgggagagg ccggaaacga ctgtccctca gataacttgg gtggtccatc gagggaacgt
     1261 cggtatcggt gatgacgtag aaaaggacgc tggggtccgg ggacctcggc tagtatggcg
     1321 agtccagcgg ccatgttcga gcgttcggtt gatccggggg gcgcccgtcc gtcggcgggg
     1381 ttcagcctcg cccgcgtcga gtacgagctg cagctccatg ccgcgctgct ccgcacgatg
     1441 cgggcgatgc tgcagaccca gcgcgtgcag cgcgagatcg accaccccgc cctgccgccg
     1501 atcctcgagg aactcgacgc gctcgagcac cacatcgccg agctgccgca ccgcgcggac
     1561 gatcggccga tgctcgcgct ggcgcgccgg ctgggctgcg gcgaccacga gatcgggttc
     1621 ctgtggacgg cggtcgcgct ggcggtcaac ccccgcgtgc tggtccacgc gcgcgggctc
     1681 gacgacacgg cgtcgcgcgg gctgtcggtg tcgctgtacg gccggatcgc caacctggag
     1741 gccgacgcgt cgcgcgcgct cgggctgtcg ctcttgcccg gccacccgac ggcgcgccac
     1801 gggctgctgg tgccggcgcg cggcgagtgg gtgccgg
//
LOCUS       DASVOR010002139         3649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_257196,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002139 DASVOR010000000
VERSION     DASVOR010002139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257196"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            89..1603
                     /locus_tag="VF516_39720"
     CDS             89..1603
                     /locus_tag="VF516_39720"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8113947.1"
                     /translation="MAPAVAAEVAKLRDRLAQARTLEQLGRYDESLAVARDASTATER
                     LGYSPVHAEALVQVARALGERSTADTRKEAQKLYFDALTLAEAERHDQLTVEIWTQLV
                     RLAARMDSNMAQAHEWWGQAYAWSRRNAPMLRLPGDDVDNQAELHYLLGQIYFRESEY
                     GKAADEERRAIAAISVGSAHSLELSHYNAELANALERMGDVGKAIQLYERALAIATGA
                     LGATHPRVLMLRINYGIALKIAGRHDEARVVLQDALASMVPQYRDAHLDAARIHSLLS
                     ELDYGQGHLDRAAGHARMSLQIYERALSPEHVRTAEAYTNLANVEFMRRNYTEALTLY
                     EHALMLRRRHFGGDHYQVGVAEVSVAETLFMLEHHDDAMVHVHEAERIFTRGSAREPE
                     IQAWLFTVQGEILVAQQKFSAAVPVLERALGQFSDYVADQANHALAMWALARALREVG
                     GDRNRVRSLAERAHTIFAGLGPTEAQERDAVAHFLAGLLQQGPEKRSDAGLGIK"
     gene            2003..>3649
                     /locus_tag="VF516_39725"
     CDS             2003..>3649
                     /locus_tag="VF516_39725"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8113948.1"
                     /translation="MFDKLHLLSQDVPEGGDGFRWRAHPYLRIVGSMPRGPAVFEWSA
                     RQRADLEALWRDPTSDGARERLAKDLAVFCEKLGWQPDVKVLEDAEQNSEEYILTLSS
                     VPPELYILPWEVVQVGADGRYLSDYASAQVRYAMPGLEARGVAKAPDKPGVLFAWSRA
                     GGEVPNEEQAAAIRAAAEAGGVSFQEVADVDEVNLQAALDAGAPSVLHLLCHGRPGAD
                     GEPSRLSWGPADNPSEIPATRLARLLRRYDSAIQLVVLSTCGSGDGHDNPMLLGSLAQ
                     ELHRKGIRSVVASRYPLSVCGSCVMTRSLYDKMLREAWSLERALRYTREALLRVADHG
                     ETHPGDAYGIQLYAHDTERFVSDNAVEAERPVLATYPFGVAARPVPAHAPPSTELTLV
                     MEADPTLSEEELVAKLRQVAEDDTLTVAIPLGDGKKRTLSVHTTADGAQRLLGAWRSR
                     ALHVVIGVAVGRLLLAKGVAKALQSLFSAFSGKVGALAGHVGGGAEPGGVKVIGGKIG
                     VNAARVAKATGQVAGKVGAAGQAGVAGQVMAGEAAIAGQVA"
BASE COUNT          594 a         1165 c         1366 g          524 t
ORIGIN      
        1 ccggtcgtcc aggtcgtcgg cgtgtgcaag cacccggggc tgttcgacgg cgacgaccac
       61 gcgatcacca acggcttcac ggtgaccgat ggcaccggcc gtcgcggccg aggtcgccaa
      121 gctccgcgac cggctcgcgc aggcgcggac gctggagcag ctcgggcgct acgacgagtc
      181 gctggcggtc gcgcgcgatg ccagcacggc gaccgagcgg ctgggctatt cgccggtgca
      241 cgccgaggcg ctggtccagg tggcgcgcgc gctgggggag cggagcaccg ccgacacgcg
      301 caaggaggcc cagaagcttt acttcgacgc gctcaccctc gccgaggccg agcgccacga
      361 tcagctgacc gtcgagatct ggacccagct ggtgcggctg gcggcgcgga tggactcgaa
      421 catggcgcaa gcgcacgagt ggtggggcca ggcgtacgcg tggtcgcggc gcaacgcccc
      481 catgctgcgc ctgccgggag acgatgtaga caaccaggcc gagctgcact atctgctggg
      541 ccagatctac tttcgcgaga gcgagtacgg gaaagcggcc gacgaagaac gtcgagcgat
      601 cgccgcgatc tcggtcggat ctgctcactc gctcgagctc agccactaca acgcagagct
      661 tgcgaacgcg ctggagcgaa tgggcgacgt tggcaaggcg atccagctgt acgagcgcgc
      721 gctcgcgatt gccactggag cgctcggcgc gactcacccg cgagtgctca tgctcaggat
      781 caactatggg atagcgctga agatcgccgg gcggcacgat gaggcgcgcg tcgtgctgca
      841 ggacgcgctg gcaagcatgg ttccgcagta tcgcgatgcg cacctggacg ccgccaggat
      901 ccacagcctc ctctcggagc tggactatgg ccagggacac ctcgatcgtg ctgccgggca
      961 tgcgcgtatg agcctgcaga tctacgagcg tgcgttgtcg cctgaacacg tgcgcaccgc
     1021 agaagcttac acgaacctgg ccaatgtcga gttcatgcgt cgaaactata cggaggcgtt
     1081 gacgctgtac gaacacgcgc tgatgctccg ccgccgccac ttcggtggcg accactacca
     1141 ggtcggtgtg gccgaggtca gcgtggcaga gacactgttc atgctcgaac accacgacga
     1201 tgcgatggtc cacgtccacg aggcagagcg catcttcaca cgcggctcgg ctcgcgaacc
     1261 cgagatccag gcatggctgt tcaccgtgca aggcgagatc ctcgtggccc agcagaagtt
     1321 cagcgcggcg gttccggtgc tcgagcgcgc gctggggcaa ttcagcgact acgtcgccga
     1381 ccaggccaat catgctctgg cgatgtgggc actcgcccgc gcgctgcgtg aggtcggggg
     1441 cgatcgaaac cgcgtccggt cgctcgccga gcgtgcccac accatctttg caggactcgg
     1501 tccgacggaa gcgcaagagc gcgacgccgt ggcgcacttc ctcgctggac tgctacagca
     1561 agggcccgag aaacgctctg atgcgggcct gggaatcaaa tgacacagcc gcaacgatga
     1621 tgttgcgata ccgactgatc aactctgcgt acgaaggaga tacccatgtc gaaccctcag
     1681 aacaacgaga ccaaggccag cgcggcgtcc tccaagcact ccccggctcc ggatggcaag
     1741 tcccagcttc cctcttcgct tccggacttc cctacgccag tagggggcgg tggcaccgac
     1801 ccgagcccca cgcccgaccc gacgccccag tcaccctgac aagctcgcaa ggggcaggtc
     1861 gcgaagccat gccagcgccc acgttgacga ctcggatcct cgaggttcgt gccgtccgtg
     1921 tgcgctgtcg cgggtctcgg atgcgtccta tcttcgccgg cttcgcccgg cgggctgaac
     1981 accctcgcag agagatcgcc tcatgttcga caagctccat ctgttaagtc aggacgtacc
     2041 ggagggaggc gatgggtttc gctggcgagc gcatccgtac ctgcgcatcg tcgggtcgat
     2101 gccgcggggg cccgcggtgt tcgagtggag cgcgcggcag cgcgccgatc tggaggcgct
     2161 gtggcgtgac ccgacgagcg acggggcgcg cgagcggctg gccaaggacc tcgcggtgtt
     2221 ctgcgagaag ctcggctggc agcccgacgt gaaggtgctg gaggacgccg agcagaacag
     2281 cgaggagtac atcctgacgc tcagctcggt gccgccggag ctgtacatcc tgccgtggga
     2341 ggtggtgcag gtcggcgcgg acggccggta cctgtcggac tacgcgagcg cgcaggttcg
     2401 ctacgcgatg ccggggctgg aggcgcgggg cgtggcgaag gcgccggaca agccgggggt
     2461 gctgttcgcg tggtcgcgcg cgggcggcga ggtgccgaac gaggagcagg cggcggcgat
     2521 ccgggcggcg gccgaggcgg gtggggtctc cttccaggag gtcgcggacg tcgacgaggt
     2581 caacctgcag gcggcgctcg acgccggggc tccgtcggtg ctgcatctgc tgtgtcacgg
     2641 gcggccgggc gccgacggcg agccgtcgcg gctgagctgg ggtccggccg acaatccgtc
     2701 ggagatcccg gcgacgcggc tggcgcgcct gctgcgccgc tacgacagcg cgatccagct
     2761 ggtggtgctg agcacgtgcg gcagcggcga cgggcatgac aatccgatgc tgctggggag
     2821 cctggcgcag gagctgcaca ggaagggcat ccgcagcgtg gtggcgtcgc gctatccgct
     2881 gagcgtgtgc ggctcgtgcg tgatgacgcg atcgctgtac gacaagatgc tgcgcgaggc
     2941 gtggtcgctg gagcgcgcgc tgcgctacac gcgcgaggcg ctgctgcggg tcgccgacca
     3001 cggcgagact caccccggcg acgcctacgg gatccagctc tacgcgcacg acaccgagcg
     3061 gttcgtctcg gacaacgcgg tcgaggccga gcggccggtg ctggcgacct atccgttcgg
     3121 cgtcgcggcg cgcccggtgc cggcccacgc accgccgtcg accgagctga cgctggtgat
     3181 ggaggccgat cccacgctgt cggaggagga gctggtcgcg aagctgcggc aggtggccga
     3241 ggacgatacg ctgacggtgg cgatcccgct gggggacggc aagaaacgca cgctgagcgt
     3301 gcacacgacg gcggacgggg cgcagcggct gctcggcgcg tggcggtcga gggcgctgca
     3361 cgtggtgatc ggcgtcgcgg tcgggcggct gctgctggcc aagggggtcg cgaaggcgct
     3421 gcagagcctg ttctcggcgt tttcgggcaa ggtcggagcg ctcgcgggcc atgtcggagg
     3481 gggcgccgag ccgggcggcg tcaaggtgat cggaggcaag atcggggtga acgcggcgcg
     3541 ggtggcgaag gcgaccggcc aggtcgcggg caaggtcgga gccgcggggc aggccggcgt
     3601 ggcaggccag gtgatggcgg gcgaggcggc catcgcgggc caggtggcg
//
LOCUS       DASVOR010002140         2018 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_401996,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002140 DASVOR010000000
VERSION     DASVOR010002140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401996"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="VF516_39730"
     CDS             <1..779
                     /locus_tag="VF516_39730"
                     /EC_number="4.2.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-dehydroquinate synthase family protein"
                     /protein_id="HEX8113949.1"
                     /translation="GVVGDLAGFVAATLYRGIAVIQVPTTIVAMTDAAIGGKTAVDLP
                     AGKNLVGAFHQPRLVACALETLVTLPGRERRAGFGELWKYALLDGPELWTQVVTCAGW
                     AAGPDATPPPPELRTVIERAISFKAAIVGRDERERTGHRALLNLGHTVGHAIESATGM
                     LHGEAVGLGLIAACRVSAAVCGTRRDLEHEVVAALRASGLPGDLDPYLTDDVLARIQV
                     DKKRIGGNLRFLVIREVGVCEPVDIALPELRTILRPDPGA"
     gene            802..1560
                     /locus_tag="VF516_39735"
     CDS             802..1560
                     /locus_tag="VF516_39735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113950.1"
                     /translation="MQGPTGTLTTPGLVGLAAVAAVAALGGCVASGEDGSMLVLKSVA
                     PPITATGGVCMFMPSDTEGELPRGVLDVSGGVGYQFVAQVASRITADPGQEAARTIFF
                     RGANVDLTFPDAGALGAQIADLQSKGLLHFMSSISGDLPPNGLADKSFELIPRGVAAE
                     LNTAAGFTSTVVQATFTVVGDMGGGNVSSQPFHYSVTLGKQNLITDLGPCSSVSKSFM
                     PHTGNLCAPGQDFPVDCCESSATTLKCPAVGTGP"
     gene            1681..>2018
                     /locus_tag="VF516_39740"
     CDS             1681..>2018
                     /locus_tag="VF516_39740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113951.1"
                     /translation="MFAETLKKVVDNIEGGIAAVIMGLDGIAVDSYVKLDDRVDVNTV
                     AMEFSFILSQVRKAGDSLSVGNLEELSVKAQRLLLVCRMLSPQYFIAIVMAPEGNFGK
                     ARYLARLAHPA"
BASE COUNT          292 a          725 c          701 g          300 t
ORIGIN      
        1 gcggcgtggt cggcgacctc gccggcttcg tcgcggctac gctgtaccgc gggatcgccg
       61 tgatccaggt cccgaccacg atcgtcgcga tgaccgacgc cgcgatcggt ggcaagaccg
      121 cggtcgacct gccggccggc aagaacctgg tcggcgcgtt ccaccagccc cggctggtcg
      181 cctgtgcgct cgagaccctg gtgacgctgc ccgggcgcga gcgccgcgcc gggttcgggg
      241 agctgtggaa gtacgcgctg ctcgacggcc ccgagctgtg gacgcaggtc gtgacctgcg
      301 cgggctgggc ggcgggcccc gacgccacac cgccgccgcc cgagctgcgc accgtgatcg
      361 agcgggcgat ctccttcaag gcggcgatcg tcggccgcga cgagcgcgag cgcaccggcc
      421 accgcgcgct gctcaacctc ggccacaccg tcggccacgc gatcgagtcg gcgaccggca
      481 tgctgcacgg cgaggcggtc ggcctgggcc tgatcgcggc gtgccgggtg tcggccgcgg
      541 tgtgcggcac ccgccgcgac ctcgagcacg aggtggtcgc ggcgctgcgc gcgagcggcc
      601 tgccgggcga cctcgacccc tacctcaccg acgacgtgct cgcccggatc caggtcgaca
      661 agaagcggat cggcggcaac ctccggttcc tcgtgatccg cgaggtgggg gtgtgtgagc
      721 cggtcgacat cgcgctcccc gagctgagaa caattttgcg tcccgatccg ggcgcttgat
      781 acgatttccg acggaggaac catgcaaggc cccaccggca cgctcacgac ccccggcctg
      841 gtcggcctcg cagcggtcgc agccgtcgca gccctcgggg gctgcgtggc cagcggggaa
      901 gacgggagca tgctcgtgct caagagcgtc gcgcccccca tcacggccac cggcggcgtg
      961 tgcatgttca tgccgtcgga caccgagggc gagctcccgc gcggcgtgct cgacgtcagc
     1021 gggggcgtcg gctatcagtt cgtcgcgcag gtcgcgagcc ggatcaccgc cgatcccggc
     1081 caggaggccg cgcggacgat cttcttccgc ggcgccaacg tcgacctcac gttccccgac
     1141 gcgggggcgc tcggcgccca gatcgccgac ctgcagagca agggcctcct gcacttcatg
     1201 tcgtcgatct ccggggacct gccgcccaac ggcctggcgg acaaatcgtt cgagctgatc
     1261 ccgcgcgggg tcgccgccga gctgaacacc gcggcggggt tcacctcgac ggtcgtccag
     1321 gcgacgttca ccgtcgtcgg cgacatgggc ggcggcaacg tgtccagtca gccgttccac
     1381 tactcggtga cgctgggcaa gcagaacctg atcaccgacc tcgggccgtg ctcgtcggtc
     1441 tcgaagagct tcatgccgca caccgggaac ctctgcgccc ccggccagga cttccccgtt
     1501 gattgctgcg agagctcggc gaccacgctg aagtgcccgg ccgtcggcac cggaccgtga
     1561 tctgcgcgca cgccctgcgg cggccgatca ctcccccgcc cggcgccccg gccggcgatt
     1621 gcggtcccgg gctatcgatc caggccagca ggtctagtac cgtgacgcca aaggtggaac
     1681 ttgttcgctg aaacgctcaa gaaggttgtc gacaacatcg aaggcggaat cgccgcggtg
     1741 atcatgggcc tcgacggcat cgccgtcgac agctacgtca agctcgatga ccgcgtcgat
     1801 gtcaacaccg tcgcgatgga gttctcgttc atcctgagcc aggtccgcaa ggccggggac
     1861 agtctgtcgg tgggcaacct ggaggagctc tcggtcaagg cgcagcggct gctcctggtg
     1921 tgccggatgc tgtcgccgca gtacttcatc gcgatcgtga tggcgcccga gggcaacttc
     1981 ggcaaggcgc gctacctggc ccggctggct catcccgc
//
LOCUS       DASVOR010002141         2728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_227578,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002141 DASVOR010000000
VERSION     DASVOR010002141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227578"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VF516_39745"
     CDS             complement(<1..1179)
                     /locus_tag="VF516_39745"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEX8113952.1"
                     /translation="MLGRAARADRPHKPVLHGQHWVAITGKPLGAQAGAMMFQRGGNA
                     VDATCAMLAAVTTLWDTLSWGGETQALIYDPTRKKVIGINALGVAPTGATPEFFKNQN
                     MKYPPEFGPLAAVTPGTPGGLFTMLAEFGTMSLAEVLGPAIQLADGYPIETELSNVIE
                     RNKAKIKQWPYAKAVLLPHQGQAHEAPAPGEIFRQPDLAATLRKLVEAEAQALKAGKN
                     RKQAIYAAYDRFYKGDIADELVRSVKEQGGLFTRDDLAKWQVHIEEPVSTTYRGITVY
                     KLTTWVQGPAMLQALNILENMDLKAMGYNSARYLHAVYQAMSLAFADRDFYYGDPYVP
                     PAEPVKGLLSKAYAKERFKQINWSKNDPDIGPGDPYPYQGEKNPFRNLLERWRATGAG
                     A"
     gene            complement(1310..2695)
                     /locus_tag="VF516_39750"
     CDS             complement(1310..2695)
                     /locus_tag="VF516_39750"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEX8113953.1"
                     /translation="MANTTTAADSFGRLRRILGVGFGLAVNVGSMIGVGIVRASGQVA
                     GQLHTVTVILAVWLLGGLYTLVGASCLTELGALLPQAGGYYVYARRAFGDRVGFAVGW
                     TDWIMYCLVLGYVSIGMSEILAELVPDAAGAKRAIAIAILVGFIGLQWAGVRISSWFQ
                     EWTTALKFLAFLALVIAALAVSGLGTGPAAAAGPAAPLTLPGLVAALQVVTITYAGWQ
                     SALYFTEEDRDPVKNLPRAMIGGVIAVAVIYLLVNLAMVSIVPIPELARATVPAAEAA
                     SRIAGVRGGQILTALSLISLPPLLNAIMMIGTRILFALGRDGLLWPRLAAVNARGTPG
                     AATLATTVIAVILILAFGDFTQLVAITAIYLALNYAVACLALVVLRRREPSLPRPVRA
                     WGYPWSAAIVIAGAIAFLVGVVIADTRSAVIALGLLAIGLVLHAVLPRRWRVPLPTAP
                     TVPAARVVDRD"
BASE COUNT          429 a          919 c          998 g          382 t
ORIGIN      
        1 cgcgccggcg ccggtggcgc gccagcgctc gagcaggttg cggaacgggt tcttctcccc
       61 ctgatacgga tacgggtcac ccgggccgat gtcgggatcg ttcttgctcc agttgatctg
      121 cttgaaccgc tccttggcgt acgccttgga cagcaggccc ttgaccggct cggccggcgg
      181 gacgtagggg tcgccgtagt agaagtcgcg atcggcgaac gcgaggctca tcgcctggta
      241 gaccgcgtgg aggtagcgcg cgctgttgta tcccatcgcc ttgagatcca tgttctcgag
      301 gatgttcagc gcctgcagca tcgccggccc ctggacccag gtggtgagct tgtagacggt
      361 gatgccgcgg taggtggtcg acaccggctc ctcgatgtgg acctgccact tcgccagatc
      421 gtccctggtg aacagcccgc cttgctcctt gaccgagcgc accagctcgt cggcgatgtc
      481 gcccttgtag aaccgatcgt aggcggcgta gatcgcctgc ttgcggttct tgccggcctt
      541 gagcgcctgg gcctcggcct cgaccagctt gcgcagcgtc gcggcgaggt cgggctggcg
      601 gaagatctcg ccgggcgccg gcgcctcgtg cgcctggccc tggtgcggca gcaacaccgc
      661 cttggcatac ggccactgct tgatcttggc cttgttgcgc tcgatgacgt tggacagctc
      721 ggtctcgatc ggatagccgt cggcgagctg gatcgcgggc cccaggacct cggcgaggct
      781 catcgtgccg aactcggcga gcatcgtgaa cagcccgccc ggcgtgcccg gcgtcaccgc
      841 cgcgagcggg ccgaactccg gcgggtactt catgttctgg ttcttgaaga actcgggagt
      901 cgccccggtc ggcgccacgc cgagcgcgtt gatgccgatg accttcttcc tggtcgggtc
      961 gtagatcagc gcctgggtct cgccgcccca gctcagcgtg tcccacagcg tggtgaccgc
     1021 ggcgagcatg gcgcacgtgg cgtcgacggc gttgccgccg cgctggaaca tcatcgcgcc
     1081 ggcctgggcg ccgagcggct tgccggtgat cgcgacccag tgctggccgt gcagcaccgg
     1141 cttgtgcggc cggtcggcgc gggccgcgcg gcccagcatc agcatcgaac tcgcaatcag
     1201 gacgacggac cgggacaaca acgcggggcg ggagcgcatc gcggacctcc gtgtggggcc
     1261 gacaccttac ctcaccacgc ggaccgcgcg gcaggtgagc ggcaggtgat caatcgcgat
     1321 cgacgacgcg cgcggcgggg acggtgggcg cggtgggcag cgggacgcgc cagcggcggg
     1381 gcagcacggc gtggaggacg aggccgatcg cgagcagccc gagcgcgatc accgcggagc
     1441 gggtatcggc gatcaccacg ccgaccagga acgcgatcgc gccggcgatg acgatggccg
     1501 ccgaccacgg atagccccac gcgcggaccg ggcgcggcag cgacggctcg cggcggcgca
     1561 gcacgaccag cgccaggcac gccacggcgt agttgagcgc gaggtagatc gcggtgatgg
     1621 cgaccagctg cgtgaagtcg ccgaacgcga ggatcaggat caccgcgatg acggtggtgg
     1681 ccagcgtggc ggcaccgggc gtgccgcggg cattgacggc cgccaggcgc ggccacagca
     1741 ggccgtcgcg gccgagcgcg aacaggatgc gggtgccgat catcatgatc gcgttgagca
     1801 gcggcggtag cgagatcagc gacagcgcgg tgaggatctg accgccgcgc acgccggcga
     1861 tgcgtgacgc tgcctccgcc gccggcacgg tggcgcgggc gagctcgggg atcggcacga
     1921 tcgagaccat cgcgaggttg accagcagat agatcaccgc cacggcgatc acgccgccga
     1981 tcatcgcgcg cggcaggttc ttgaccgggt cgcggtcctc ctcggtgaag tacagcgcgc
     2041 tctgccaccc ggcgtaggtg atcgtgacga cctggagcgc ggcgaccaga ccgggcaggg
     2101 tcagcggcgc ggcgggaccg gcggcggcgg ccgggccggt gccgagaccg gagaccgcaa
     2161 gcgcggcgat caccagcgcg aggaacgcga ggaacttgag cgcggtggtc cactcctgga
     2221 accagctcga gatccgcacg cccgcccact gcaggccgat gaagccgacc aggatggcga
     2281 tcgcgatcgc gcgcttggcg ccggcggcat cggggacgag ctcggcgagg atctcgctca
     2341 tcccgatcga gacgtagccc agcaccaggc agtacatgat ccagtcggtc cagccgacgg
     2401 cgaagccgac ccgatcaccg aacgcgcggc gggcgtagac gtagtagccg ccggcctgcg
     2461 gcagcagcgc gccgagctcg gtcaggcagc tggcaccgac cagcgtgtag agcccgccga
     2521 gcagccacac cgcgaggatc actgtgacgg tgtggagctg gccggcgacc tgcccggacg
     2581 ctcgcacgat gccgaccccg atcatgctgc cgacgttgac cgccaggccg aacccgacgc
     2641 cgaggatgcg ccggagccgc ccgaaggaat ccgctgccgt ggtggtgttc gccaagctga
     2701 gcgactatac gcgcagtcat gcaccatc
//
LOCUS       DASVOR010002142         2108 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_284915,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002142 DASVOR010000000
VERSION     DASVOR010002142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284915"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..1542
                     /locus_tag="VF516_39755"
     CDS             466..1542
                     /locus_tag="VF516_39755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113954.1"
                     /translation="MRLGTCIALELAALAGAVGGVWFVASRGAELAGSYIDHHEAAAA
                     TTTDPPAAAVVPLERSQFVARREPDTVFGAPDAELLAPLAAMPVTRVKINRGGSSLSL
                     RLDFASGARAAFKPMQIHPQSDPRREIAAYRIDRLLGIGHVPPARSAAFAIEDVIAAA
                     DPGNRGVTASRIEQEALPRDGKLCGELSWWIPEIKRVRIGGRYVDEPDGEALWAEYLQ
                     AGAAIPAELRPMLAQLASVIVFDVVIDNSDRWSGNNTQGSPDDRILYFMDNTLAFSQY
                     TIGHTANLARLYRIQVFPRGLIGRVRRLSAEAVAAALGHDDVLGPLLGEGEIRAVMSR
                     RDHLVEYVDRLIADLGEDAVLALP"
     gene            1788..>2108
                     /locus_tag="VF516_39760"
     CDS             1788..>2108
                     /locus_tag="VF516_39760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113955.1"
                     /translation="MIEILHEPASGVVTSAPDRRVERRARHPDEIPLAAFWAADRAEK
                     AERAEKAEKERSGAVRAGAVPQDDARAGHDAAVTARRDAAERGNRDSAEASEGRRAAA
                     ADAAG"
BASE COUNT          282 a          730 c          810 g          286 t
ORIGIN      
        1 cagcgtgtcg gcgatcccgc acaccgtgat catcgatcgc cgcggcgtgg tgcgcgaggt
       61 cgtgcgcggc accgcggacg tcgcggcgat catcgagacg atccgcgcgg ccgagtgagg
      121 ccgcgtttgc agaagtggtt atcgaagtgc gccgcgtggt gcgcgggcgg aggctcgtgc
      181 tacgacggag gacatggcca gcatggtctg cgccacgtgc cgcaagttga tcccaccggg
      241 cgcttcggcg gtccggtgta cggtcgcgtc gtgcaatgcc ggccgcatga agcttcggtt
      301 ctgctcggtc gcgtgctggg agaagcacgt gccgaccgcc cgccaccgca agcccgccta
      361 cgtgatcgag gtccgggtcg acggagagta gccgcgccgg tagggcgaag cggggcgaat
      421 cgcgacaaac tgccgcctcg atcggcggcg tgatagcttt cgggggtgcg actcgggacg
      481 tgcatcgcgc tcgagctcgc cgcccttgcg ggtgccgtgg gcggtgtgtg gttcgtcgcg
      541 tcgcgcggcg ccgagctcgc cggctcgtac atcgaccacc acgaggccgc cgccgccacc
      601 acgaccgacc cgccggccgc ggccgtggtg ccgctggagc gctcgcagtt cgtggcgcgg
      661 cgggagcccg acacggtgtt cggcgcgccg gacgccgagc tgctcgcgcc gctcgcggcc
      721 atgccggtca cccgggtcaa gatcaaccgc ggcggctcgt cgctgtcgct gcggctcgac
      781 ttcgcgtcgg gggcgcgcgc cgcgttcaag ccgatgcaga tccatcccca gtccgatccg
      841 cggcgcgaga tcgcggcgta ccgcatcgac cggctgctcg gcatcggcca cgtcccgccg
      901 gccaggagcg ccgcgttcgc gatcgaggac gtgatcgcgg cggccgaccc cggcaaccgc
      961 ggcgtcaccg cctcgcggat cgagcaggaa gcgctcccgc gcgacggcaa gctgtgcggg
     1021 gagctgtcgt ggtggatccc cgagatcaag cgggtccgga tcggcgggcg ctacgtcgac
     1081 gagcccgatg gcgaggcgct gtgggccgag tacctgcagg ccggtgccgc gatccccgcc
     1141 gagctccggc cgatgctcgc ccagctcgcg tcggtgatcg tgttcgacgt cgtgatcgac
     1201 aactccgatc ggtggagcgg caacaacacg cagggctcgc ccgacgaccg catcctgtac
     1261 ttcatggaca acacgctcgc gttctcgcag tacacgatcg gccacaccgc gaacctggcg
     1321 cggctgtacc ggatccaggt gttcccgcgc gggctgatcg ggagggttcg caggctgagc
     1381 gccgaggcgg tggccgctgc gctcggccac gacgacgtgc tcggcccgct gctcggcgag
     1441 ggcgagatcc gcgcggtcat gtcgcgtcgc gatcacctcg tggagtatgt cgaccggctg
     1501 atcgccgacc tcggcgagga cgccgtgctg gccctgccat gacgacctgt ccggcctgcg
     1561 acagctccgt cgatccgctg cgctcgcgat tcgtcggcgt gtgcgacggc aagatcgtgg
     1621 cctactgctc ggccgagtgc gccgccggtc gaccgcgcgc tcaggccggg cgggcgatgt
     1681 cgccggcgac cggcgtgccg gcgcgcatcc cgacgccgcc ggtcggcgtg cctgggaccg
     1741 gtggaccgcc ggatggactg gcggggggat cgatcgacag cgggccggtg atcgagatcc
     1801 tgcatgagcc ggcgtcgggc gtggtgacca gcgcgccgga ccggcgggtc gagcggcgcg
     1861 cccggcatcc cgacgagatt cccctcgccg cgttctgggc cgccgaccgg gcggagaagg
     1921 ccgagcgagc cgagaaggcc gagaaagagc gatcgggggc ggtccgcgca ggcgccgtgc
     1981 cgcaggacga cgctcgcgca gggcacgacg cggcggtgac ggcacgacgc gacgcggccg
     2041 agcgcgggaa ccgcgattcc gcggaagcgt cggaggggcg gcgcgcggcg gccgcggatg
     2101 cagcaggg
//
LOCUS       DASVOR010002143         2646 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_139794,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002143 DASVOR010000000
VERSION     DASVOR010002143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2646
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139794"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VF516_39765"
     CDS             <1..420
                     /locus_tag="VF516_39765"
                     /inference="COORDINATES: protein motif:HMM:NF019935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113956.1"
                     /translation="RERIERELGGPASRRDVKAGAGGVIDIEFAAQYLQLVHGHAHPA
                     LRTTGTVTALEAAAAAGIAPARDLELLAQGYRFLRKVEHRLRVVHDQPIHRLPEARDE
                     LDKLARRCGFPDGGVLLEHVERWQHDVRSAYLRLVAA"
     gene            532..1455
                     /locus_tag="VF516_39770"
     CDS             532..1455
                     /locus_tag="VF516_39770"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF005146.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HEX8113957.1"
                     /translation="MGIKKLDTIWLDGSLVPWDQATDHLLAHTMHYGVGAFEGIRAYQ
                     RADGRSAIFRLREHIERLLDSCTICTIDCPYGRDPLMAACTEVVRANRIASCYLRPLV
                     YLGYGALGLGSLESPVRTMVACYEWGAYLGDDGLRRGIRCMVSGFTRANSNAVMNKGK
                     ICGQYVPSVLAKRMAGKSGFEEALMLDPQGYVAEGTGENIFLIKHGVVRTPPTSSAIL
                     AGITRDTTIQLLREQGVEVREEPISRDELYIADEVFLTGTAAEITPVREIDHRRIGRG
                     EAGPVTRRLQEAFFSIVKGSDSRHDHWLTYL"
     gene            1660..>2646
                     /locus_tag="VF516_39775"
     CDS             1660..>2646
                     /locus_tag="VF516_39775"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8113958.1"
                     /translation="MDLAPGTLLLGKYRIDGLIGTGGMGHVVRASHLYLHQPVAIKLL
                     LPALTQSADTVQRFLREAQATVKLRSEHSARVMDVGTTDEGVPFMVMEYLDGNDLNQI
                     LRHHGPQAPAIVVDLLLQACEGIAEAHALGIIHRDIKPSNFFITRRPDGSMLLKILDF
                     GISKTPVGYEELTGTQTVIGTPSYMAPEQMKSGRAADPRSDIWSLGVVMYQLIVGHPP
                     FSGESYAELVLKVGLEAPAPIPVPVPTGLDDVIMRCLEKDPNQRHQSVGELARMLAPF
                     STDPIAAAQSASRAMRTLQQRSSLQGMGSPLTAGGGLATPIPISPAQLTPRSW"
BASE COUNT          379 a          952 c          920 g          395 t
ORIGIN      
        1 cgcgagcgga tcgagcgcga gctcggcggt ccggcgagcc ggcgcgacgt caaggccggc
       61 gccggcggcg tcatcgacat cgagttcgcc gcgcagtatc tgcagctggt ccacggccac
      121 gcccacccgg cgctgcgcac caccggcacg gtgacggcgc tcgaggccgc ggcggcggcc
      181 gggatcgctc ccgcgcgcga tctcgagctg ctcgcccagg gctaccggtt cctgcgcaag
      241 gtcgagcacc ggctgcgcgt ggtgcacgat caaccgatcc accggctgcc ggaggcgcgc
      301 gacgagctcg acaagctggc gcggcgctgc gggttccccg acggaggtgt gctgctcgag
      361 cacgtcgagc gctggcagca cgacgtccgg tccgcgtacc tccgactggt cgccgcgtga
      421 ccggccgtcg ctgcgtagcc agcttcgcag ccgcggtgcg tcggctgtac cgaccgtctg
      481 gtcggagaac tagcgcttgt gattgcagcg gtgcgttgct acgaagcggg catgggcatc
      541 aagaagctcg acacgatctg gctcgatggt tcgctcgtgc cgtgggacca ggcgacggat
      601 cacctgctcg cccacacgat gcactatggc gtcggcgcgt tcgagggcat ccgcgcctac
      661 cagcgcgccg acggtcgctc cgcgatcttc cgcctgcgcg agcacatcga gcggctgctc
      721 gactcgtgca cgatctgcac gatcgactgc ccgtacgggc gcgatccgct gatggcggcg
      781 tgcaccgagg tcgtgcgcgc caaccggatc gcgtcgtgct acctgcggcc gctggtctac
      841 ctcggctacg gcgcgctcgg cctgggctcg ctcgagtcgc cggtgcgcac catggtcgcg
      901 tgctacgagt ggggcgccta cctcggcgat gacgggctca ggcgcggcat ccgctgcatg
      961 gtctcgggct tcacccgcgc caacagcaac gcggtgatga acaagggcaa gatctgcggc
     1021 cagtacgtgc cctcggtgct cgccaagcgg atggccggca agagcgggtt cgaggaggcg
     1081 ctgatgctcg acccgcaggg ctacgtcgcc gagggcaccg gcgagaacat cttcctgatc
     1141 aagcacggcg tggtgcgcac cccaccgacg tcgagcgcga tcctcgccgg catcacccgc
     1201 gacaccacga tccagctgct gcgcgagcag ggcgtcgagg tccgtgagga gccgatctcg
     1261 cgcgacgagc tgtacatcgc cgacgaggtg ttcctgaccg ggactgcggc ggagatcacg
     1321 ccggtccgcg agatcgatca tcgccggatc ggccgcggcg aggccggccc ggtgacccgc
     1381 cggctccagg aggcgttctt ctcgatcgtc aagggctcgg attcccggca cgatcactgg
     1441 ctgacgtacc tctaactccg tcgatccgcg tctcactccc cgtctctggg ggcctggggg
     1501 cctgcagcgt cttgctcgtc ttggcccgtc tcgggcccgt ctcgggcccg tctcggacgc
     1561 tttgggcccc ggcgcacgtt tggcctggcg gcgcggcccg ccaggggtcg tcgcgcggtt
     1621 cggccggacc gggcgcggca agctgctaca ttccccgcag tggacctggc gcctgggacc
     1681 ctcctgctgg gcaagtaccg gatcgacggt ctgatcggga ccggcgggat gggccacgtc
     1741 gtgcgcgcct cgcacctcta cttgcaccag ccggtcgcga tcaagctgct gctccccgcg
     1801 ctgacccaga gcgccgatac ggtgcagcgc ttcctgcgcg aggcccaggc cacggtcaag
     1861 ctgcgcagcg agcacagcgc gcgggtcatg gacgtgggca cgaccgacga gggcgtgccg
     1921 ttcatggtga tggagtacct cgacggcaac gacctcaacc agatcctgcg ccaccacggg
     1981 ccgcaggcgc cggcgatcgt cgtcgacctg ctgctccagg cgtgcgaggg catcgccgag
     2041 gcccacgcgc tcggcatcat ccaccgcgac atcaagccgt cgaacttctt catcacgcgg
     2101 cggcccgacg gctcgatgct gctcaagatc ctcgacttcg ggatctccaa gacgccggtc
     2161 ggctacgaag agctcaccgg cacccagacc gtgatcggca cgccgagcta catggcgccc
     2221 gagcagatga agagcggccg cgccgcggat ccgcgcagcg acatctggtc gctcggcgtg
     2281 gtgatgtacc agctgatcgt cgggcacccg ccgttctccg gcgagagcta cgccgagctc
     2341 gtgctcaagg tcggcctcga ggcgcccgcg ccgatcccgg ttccggtgcc gaccgggctc
     2401 gacgacgtga tcatgcggtg cctggagaag gaccccaacc agcgccacca gagcgtcggc
     2461 gagctcgcgc gcatgctggc gccgttctcg accgatccga tcgcggcggc gcagtccgcg
     2521 tcgcgggcga tgcgcaccct gcagcagcgc agctcgctgc agggcatggg ctcgccgctg
     2581 accgccggcg gcgggctggc gaccccgatc ccgatctcgc cggcccagct cacgccgcgc
     2641 agctgg
//
LOCUS       DASVOR010002144         5659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_284932,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002144 DASVOR010000000
VERSION     DASVOR010002144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_284932"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            685..3375
                     /locus_tag="VF516_39780"
     CDS             685..3375
                     /locus_tag="VF516_39780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113959.1"
                     /translation="MRPLALSPDRQHLFAVNTPDNRLEIFRIKKTQLIHAASIPVGLE
                     PVAVAARTDHEVWVVNHLSDSVSVVELGDDATTGHVERTLLVGDEPRDIVFAGHHRGR
                     AFITTAHRGQNIPFDPQFTTPGVGRADVWVFDANALGNSLGGTPLTIVTLFTDTPRAL
                     AVTPDGTRVYAAGFQTGNRTTILNEALIPNGFGPDGIPGPATNFEGRPAPETGTIVQW
                     NGSHWIDALGRIRNEPVKLALPDKDVFVLDAMADPPHQLPGSGGFFQGVGTILYSMIV
                     NPVNGKVYVANTDANNLDRFEGPGIFAGHSLRGHLHENRITVLTPGGDVAPRHLNKHI
                     DFSRCCAPIPNDENARSLALPQGMAVTGNGATLYVAALGSDKVGVFSTAQLENDTFVP
                     NAANQIHVTGGGPTGLALDENRGQLYVLTRFDNAISIIDTATRAETAHVAMHTPEPPS
                     VIAGRRFLYDASFSSSHGDSSCASCHVFGDFDSLAWNLGNPDEKVTPDPGPFTSELFN
                     LLTQQPIDPIFHPMKGPMTTLSLRGMANHGPMHWRGDRTGGNDAPTAQPDSGTFDERA
                     AFDKFRAGFTDLLGRDSFIPPADMDAFTDFILQVTYPPNPNRPLDNVLTPDQEAGRQL
                     FSGVNCGIPTPDGTPNVLTCISCHMIDPSGNPDSAAPGFFGTAGFSSFAFQTQLFKIP
                     HIRNLYQKLGMFGNAIVPGNLSLDDAFTGDQVRGFGFNHDGAIDTLFRFHHGISFSEQ
                     ATGVGNGGIPDGSAGEAQRRQLEAFLLAAPTNLAPIVGQQITLTPSSPAAVVSRVELL
                     RQRADAGECDLVAKAEIAGGEAGFVYVGSGLFQGDRRALPPITNAALQRLATHGRAVT
                     YTCVPPGSGQRIGVDRDGDGSWDGDERDAHTDPADPNSAP"
     gene            complement(3389..4471)
                     /locus_tag="VF516_39785"
     CDS             complement(3389..4471)
                     /locus_tag="VF516_39785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8113960.1"
                     /translation="MAMLANRVRKNARHLGKWARREGVTCWRVYDRDIPELPITVDSY
                     EGALVINDYRAFERSETGGGDPDGFAGGAGGRAPRGIEHSAPGDDAWLDAAVAAVTSA
                     LPPGEVFVKRRERFAHRGPEQQYERQADQGAWRTVHEGGHAFRVNLSDYVDTGLFLDH
                     RITRAKVAAEPARTMLNLFGYTGAFSVYAGAAGMQTTTVDLSNTYLAWAAENLALNRL
                     TGELVHADVRDFLAGARRAGRRWDLVVVDPPTFSNSKRMDYTWDVQRDHPALLRDALA
                     VCTGVVWFSTNRRRFQLELDDPRDATRGGVNGPALPVHPPEGGYGKAPLLIDDPELAI
                     ADLTHATIPPDFRDHKVHRAYRITRR"
     gene            complement(4490..>5659)
                     /locus_tag="VF516_39790"
     CDS             complement(4490..>5659)
                     /locus_tag="VF516_39790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEX8113961.1"
                     /translation="HAASARERRVLAELTADELRGALARLDARIAQAEAEAGEQAARL
                     VIDLAADAETDTEGEIELAIGYVVPGAAWRPYHRAVLVRGGDAATVEWQTTACVWQAT
                     AEDWRDVALVFSLERPSLGVEPPGLTDDELTARRRPDTIAVEARDHELQTTGLGGEAP
                     GAEAQVPGIDDGGLGVALTAARATVVADGAPHRVPVGGFTSPAQIDRVAIPLRSPWVH
                     VRARLVNTGAAPLLAGPVDLVMSSGYVGRTEIGFVAPSEKLQLGFGPEADVRVHRTET
                     SERDDAGLLGGWNVQTVRVAVRLSNLGNAPREVVVTERIPVSEVEQVEVKASAPDAYL
                     LGAGDPPGAEQVVQVTARAIDDRGLVTWSVELPPLGRRAVALEYRIRSQRGVAGV"
BASE COUNT          866 a         2077 c         1905 g          811 t
ORIGIN      
        1 cgcgctgcgc ctgctcgccc acctcggcca cccggtgacc ttcacctgcg tcccgcccgg
       61 ctccggcgag cggatcggcg tcgaccgcga cggcgacggc gcctgggacg gcgacgagcg
      121 cgacgcccac accgatcccg ccgacccgaa cagcacgccg tgacacgccg gacggacgcg
      181 aggcgatcgt cgggcgcccg gccgcctcac tgaacccatg ggcgccacgg tcgatcgccc
      241 gaccgccgcg gcccgtccgg catctgcgac gtcgcagaac acgtcgcaga acacgtcgtc
      301 gaacacgtcc tgagttgaca agcaagtcga atcgatctcg actcgccgcg aggatttcac
      361 tcgcaatcag cctgtgcagt gctatcatgt cactccattt ccggtcatga cggcgtattt
      421 gccacaatgc tgatgtgttg tagtcacgag ggagagagag gacagccaat gggttcgcga
      481 cacttgacct gcgcctgggt gaggccgctc gtggtcttca cgacactatc gctcttcatg
      541 acgagctctt gcgccccgcg tcccgaaacc gcacgccgcg aggaatccgc cggtaccggc
      601 gccgcgatcc gccatggatc cgcacccgag cgagtcgtac ctgcagcggc cgggccccgg
      661 ttcacgttgt tcgagagcgg ccaggtgcgt ccgctcgccc tgtctccgga cagacagcac
      721 ctgtttgccg tcaacacccc cgataaccgc ctcgagatct ttcgtatcaa gaagactcag
      781 ctcatccacg ccgcgtccat cccggtgggc ctcgagccgg tcgccgtcgc cgcgcggacc
      841 gatcacgagg tctgggtggt caatcacctg tcggacagcg tgagcgtggt cgagctcggc
      901 gacgacgcca cgaccgggca tgtcgagcgc acgctgctcg tcggtgacga gcctcgcgac
      961 atcgtgttcg ccgggcacca tcgcggccgc gcgttcatca ccacggcgca tcgcggccag
     1021 aacatcccgt tcgatcccca gttcacgacc ccgggcgtgg gacgcgcgga cgtgtgggta
     1081 ttcgatgcga acgcactcgg caattcactg ggtggcacgc cgctcaccat cgtcaccctg
     1141 ttcaccgata cgccccgcgc gctcgcggtg acgcccgacg gcacgcgggt ctacgccgcc
     1201 ggctttcaga ccggcaaccg aaccaccatc ctcaatgagg cattgatccc gaacggcttc
     1261 ggcccggacg gcatcccggg gcccgccacc aacttcgaag ggcgccccgc gcccgagacc
     1321 gggaccatcg tccagtggaa tggttcccac tggatcgatg cgctcggccg catccgcaac
     1381 gagccggtga agctcgcgct gcccgacaag gacgtgttcg tgctcgacgc catggccgac
     1441 ccgccgcacc agctcccggg ctctggcggg ttcttccagg gcgtcggcac catcctgtac
     1501 tcgatgatcg tcaatcctgt gaatgggaag gtctatgtcg ccaacaccga cgcgaacaac
     1561 ctcgatcggt tcgaaggccc tgggatcttc gcggggcaca gcctgcgcgg gcacctgcac
     1621 gagaaccgga tcaccgtgct cacccccggg ggcgacgtcg ccccccggca cctgaacaag
     1681 cacatcgact tcagccgttg ctgcgcgccg atcccgaacg acgaaaatgc ccgctccctc
     1741 gcgttgccgc agggcatggc ggtgaccggc aacggggcga cgctctatgt cgcggcgctc
     1801 ggatccgaca aggtcggggt attctccacg gcacagctcg agaacgacac gttcgtaccg
     1861 aacgcggcga accagatcca cgtgaccggc ggggggccaa cgggcctggc gctcgacgag
     1921 aatcgcggcc agctctacgt gctgacgcgg ttcgacaacg cgatctcgat catcgacacg
     1981 gcgacgcggg ccgagacggc gcacgtcgcg atgcataccc ccgagccgcc gagcgtcatc
     2041 gccggccgcc ggttcctcta cgacgcctcg ttctcgtcga gccacggcga ctcgtcctgc
     2101 gcgagctgcc atgtgttcgg cgacttcgat agcctggcgt ggaacctcgg caatcccgac
     2161 gagaaggtga cccccgaccc cgggccgttc accagcgagc tgttcaacct gctcacacag
     2221 cagcccatcg accccatctt ccatccgatg aaggggccga tgacgacgct gagcctgcgc
     2281 ggcatggcca accacggccc gatgcactgg cgcggcgatc gcaccggcgg caacgacgcg
     2341 ccgaccgcgc aacccgacag cgggaccttc gacgagcgcg cggcattcga caagttccgg
     2401 gccgggttca ccgatctgct cgggcgcgac tcgttcatcc cgccggcgga catggatgca
     2461 ttcaccgact tcatcctcca ggtcacgtat ccgcccaacc cgaatcgccc tctcgacaac
     2521 gtgctcacgc ccgatcagga ggccgggcgt cagctgttca gcggcgtcaa ctgcggcatc
     2581 ccgacgccgg acggaactcc gaatgtcctc acgtgcatca gctgtcacat gatcgatccc
     2641 agcgggaacc ccgacagcgc agcgcccggg ttcttcggga cggccgggtt ctcgagcttt
     2701 gccttccaga ctcagctctt caagatcccc cacatccgta acctctacca gaagctcggg
     2761 atgttcggga atgccatcgt cccggggaac ctgtccctgg acgacgcgtt cacgggagac
     2821 caggtgcgcg gcttcgggtt caaccacgac ggggcgatcg acacgctgtt ccggttccat
     2881 cacgggatca gcttctcgga gcaagccacc ggggtcggca acggcggaat cccggacggg
     2941 tccgcgggag aagcccagcg gcggcagctc gaggcgttcc tcctggcagc tcccaccaac
     3001 ctcgcgccca tcgtgggcca gcagatcacg ctcacgccct cgagccccgc ggcggtggtg
     3061 tcccgcgtcg agctgctccg ccagcgcgcc gacgccggag agtgcgatct cgtcgccaag
     3121 gcagagatcg caggcggcga ggccgggttc gtgtacgtcg gatcgggcct gttccagggc
     3181 gatcgccgtg ccctgccccc gatcaccaac gcggcgctgc agcggctggc cacgcacggc
     3241 cgcgccgtga cctacacctg cgtgcccccc ggctcgggac agcgcatcgg cgtcgaccgc
     3301 gatggcgatg gctcctggga cggcgacgaa cgcgacgccc acaccgatcc cgccgacccg
     3361 aacagcgcgc cctgatcgcg cgaagcgctc accgccgcgt gatgcgatag gcgcggtgga
     3421 ccttgtggtc gcggaagtcc ggcgggatgg tggcgtgggt cagatcggcg atcgcgagct
     3481 ccgggtcgtc gatcaatagg ggggctttcc catagccccc ctctggggga tggaccggca
     3541 gagccggtcc attcacccct ccgcgcgtcg cgtcgcgcgg atcatctagt tcgagctgga
     3601 accggcggcg gttggtcgag aaccacacca cgccggtgca caccgcgagc gcgtcgcgca
     3661 gcagcgccgg gtgatcgcgc tggacgtccc aggtgtagtc catccgcttg gagttggaga
     3721 acgtcggcgg atcgaccacg acgagatccc agcgccggcc ggcgcgccgg gcgccggcga
     3781 ggaagtcgcg cacgtcggcg tggaccagct cgccggtcag ccggttgagc gcgaggttct
     3841 cggccgccca cgcgaggtag gtgttggaca gatcgaccgt cgtggtctgc atcccggcgg
     3901 cgccggcgta gaccgagaac gcgccggtgt agccgaacag gttgagcatg gtgcgcgccg
     3961 gctcggcggc gaccttcgcg cgcgtgatgc gatgatcgag gaacaggccg gtgtcgacgt
     4021 agtcgctcag gttgacgcgg aacgcgtggc cgccctcgtg cacggtgcgc cacgcgccct
     4081 gatcggcctg gcgctcgtac tgctgctcgg ggccgcggtg cgcgaaccgc tcgcgccgct
     4141 tgacgaacac ctcgccgggc gggagcgcgg acgtcaccgc cgccactgcg gcgtcgagcc
     4201 aggcgtcgtc acccggcgcg ctatgctcga tcccacgggg ggctctgccc cccgccccgc
     4261 cggcgaagcc gtcggggtcc ccaccccccg tctcgctgcg ctcgaacgca cggtagtcgt
     4321 tgatcaccag cgcgccctcg tagctgtcga cggtgatcgg gagctccggg atgtcgcggt
     4381 cgtagacccg ccagcaggtg acgccctcgc ggcgggccca cttgccgagg tggcgcgcgt
     4441 tcttgcgcac ccggttggcg agcatggcca tgtcggtcat cgtcgcgggc tataccccgg
     4501 cgacgccgcg ctggctccgg atccggtact ccagcgcaac cgcgcgccgg cccagcggcg
     4561 gcagctcgac cgaccaggtc accaggccgc ggtcgtcgat cgcccgcgcg gtgacctgca
     4621 cgacctgctc ggcccccggc gggtcgccgg cgcccagcag gtaggcgtcg ggcgccgagg
     4681 ccttgacctc gacctgctcg acctcggaga ccgggatccg ctcggtcacc acgacctcgc
     4741 gcggcgcgtt gccgaggttg gacagccgga ccgcgacccg gacggtctgg acgttccagc
     4801 cgccgagcag gccggcgtcg tcgcgctcgc tggtctcggt gcgatggacg cggacgtcgg
     4861 cctcgggccc gaacccgagc tggagcttct cgctcggggc gacgaagccg atctcggtgc
     4921 gcccgacgta acccgacgac atcacgagat cgaccgggcc ggcgagcagc ggcgccgcgc
     4981 cggtgttgac caggcgggcg cggacgtgga cccacggcga gcgcagcggg atcgcgaccc
     5041 gatcgatctg cgccggcgag gtgaagccgc cgaccggcac gcggtggggc gcaccgtcgg
     5101 cgaccaccgt ggcgcgcgcc gcggtcagcg caaccccgag gccgccatcg tcgatcccgg
     5161 ggacctgcgc ctcggcgccc ggggcctcgc cgccgagccc ggtggtctgc agctcgtggt
     5221 cgcgggcctc gaccgcgatc gtgtcggggc ggcgccgcgc ggtgagctcg tcgtcggtga
     5281 ggccgggcgg ctcgacgccg agcgacgggc gctcgagcga gaacaccagc gcgacgtcgc
     5341 gccagtcctc ggcggtggcc tgccacacgc acgcggtggt ctgccactcg acggtcgccg
     5401 catcaccgcc gcggaccagg acggcgcgat ggtacggccg ccacgcggcg cccgggacga
     5461 cgtagccgat cgcgagctcg atctcgccct cggtgtcggt ctcggcgtcg gcggcgagat
     5521 cgatcaccag ccgggcggcc tgctcgccgg cctcggcctc ggcctgggcg atccgggcgt
     5581 cgagccgggc gagcgcaccg cggagctcgt cggcggtgag ctcggcgagc acccggcgct
     5641 cgcgcgccga cgcggcgtg
//
LOCUS       DASVOR010002145         4606 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_257267,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002145 DASVOR010000000
VERSION     DASVOR010002145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4606
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257267"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..189)
                     /locus_tag="VF516_39795"
     CDS             complement(<1..189)
                     /locus_tag="VF516_39795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113962.1"
                     /translation="MPDFLLVPLVASFARDDLGLRFRGQTPPAPIRAKIRLAIAEAIE
                     RRLVVEAGTDLAIGPLAEE"
     gene            complement(982..2283)
                     /locus_tag="VF516_39800"
     CDS             complement(982..2283)
                     /locus_tag="VF516_39800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HEX8113963.1"
                     /translation="MPIDETRLVCVNTPVEDLAPLRQLTELRELRMRNTNPHEPTDPR
                     VTDMSPLAGLRQLQVLELPGAPVSDLRPLAALSHLRRLDVSRSRVRELAPLAGLRTLT
                     SLDVASTAVADLAPLAGLTELEDLDVSHTQVDALGPLRDLPGLRRLVLRGAPVSDLTP
                     LADRPAITQAEALDLTDTRVRSTVALAGAAALRQLRMINAPVGDLSGLRTLHALRELR
                     LDGSEVEDLAALAGLTGLETLTLSNTRVSDVAPLARLVELRVLALDGTRVSDLAPLAG
                     LGRLEELTLSGTPVSNLASLGGLGRLRNLQLESTGVRDVSPLNGLALESLNLSHTPVD
                     LSTLGSLAELRTLALGATQLTDIRALRGATRLERLDLRATAVTDLTPLAELPELAFLD
                     LQGAAVDDEQVEQLRERRPELVIVREPPRRHDRSHPRRPRP"
     gene            complement(2326..2895)
                     /locus_tag="VF516_39805"
     CDS             complement(2326..2895)
                     /locus_tag="VF516_39805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113964.1"
                     /translation="MSEDEKVNNAAHYEELPARELGTSLYAGVTFKPGVKKGGGKVEF
                     DDEVRREASEYLRKAWDKAVDVHSFLRTIRREVEAGNPGAFASKKARILEISKLEKLT
                     VHEQSTPSTINMNDIVLSEGAAHAMTIIQNAVQKQAVPKAPVAPKVKADYVQDVIDGS
                     IKSYGALTGNAADDKALVDWLVKEYRKPL"
     gene            2926..3087
                     /locus_tag="VF516_39810"
     CDS             2926..3087
                     /locus_tag="VF516_39810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113965.1"
                     /translation="MIVDGALREVAALVDDGDLRRVGVLDFGNRALEVDVLAPEIRPP
                     RPTCSSSRL"
     gene            complement(3248..3754)
                     /locus_tag="VF516_39815"
     CDS             complement(3248..3754)
                     /locus_tag="VF516_39815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113966.1"
                     /translation="MTIAGREGREPGKTTRLAESIAPASPGKRALTDAFSADLGGHGQ
                     GMMSTGDLSGQLQAERAARQPTSNVKFRAPKSTDISAILAAGKVPEAKLKDSIALALT
                     RMAKEGQLKTKDPIADVMARIFPGAGKFDQAEFEKVVDVRTATRSTGRWSMPRPRSAP
                     PTSRSSRR"
     gene            4324..>4606
                     /locus_tag="VF516_39820"
     CDS             4324..>4606
                     /locus_tag="VF516_39820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113967.1"
                     /translation="MIHDDATPRTLYTVELTLTADPAIAGRATLRIASRGRDPVTVAI
                     PERPGGERAAIAYTILGSCRLAGVDPREYLADVLPRLTGRIRLVDLPALL"
BASE COUNT          682 a         1568 c         1578 g          778 t
ORIGIN      
        1 ttcctcggca agtgggccga tcgcgaggtc ggtgcccgct tccaccacga gacgccgttc
       61 gatcgcctct gcgatcgcga gtcggatctt cgctctgata ggtgctggcg gtgtctgacc
      121 acgaaagcgc agaccaagat catctcgggc gaagcttgcg acaagtggca cgaggaggaa
      181 gtcgggcatt ggtgcgatag caagacctct ctggatcagc tccagccatt gcggcggctc
      241 gacctgccga agagccttcc tgggggatgg ttcgtgagca gcaacagaac gcactcccgc
      301 gcgtttcgag ccccttgtca gccgagattt ctctagcttg cttccgcgct tggtcgactt
      361 ccttttccgg accataggca cccgctactt ccctacccgc tcgccaggag gtggcgcatt
      421 gcggctgctg gcggacggcc cagccgccag tgcgcaccag cgccagcccg cggtcgcgca
      481 ctgtcgaagc gaattgctgg tccggcgtcc ggccacggcg tgcttcggcc agccactatc
      541 gccgaatcgg gtagtcgttg acgtacacat gaccgccgtc atcggtgcag tgcgtggacg
      601 cgacctgctt ggtgacgcac gcacaggtcg gcttcgactt gcagctctcg ggaagaggca
      661 tgcacttctt gcgatcgatc ggccggccgc gcgcgtcgag cgcgtggccc ttgtagagat
      721 cctcgcaaaa ctgatccggc gagcagatct ccgatccaca ggctccgggc gcatcgtcgg
      781 gatcagccga ggcaggcgcg gcggacgagc cttgcgaacc cgctgacggt ggcggtgccg
      841 aagcctcccg cggcgatgtg gcgggcgctg gcggcgccgc tgggaccggc gatccggcgc
      901 cagcaggcgg cttcgccggc tccgcgccac acccacacag catggcgagt acgagggccg
      961 cacttcgcaa cgcacagcgc ttcatggccg aggcctcctg gggtgggacc ggtcgtgtcg
     1021 ccgcggcggc tcgcgcacga tgaccagctc ggggcgacgc tcgcgcagct gctcgacctg
     1081 ctcgtcgtcg accgcggcgc cttgcaggtc gaggaatgcc agctcgggca gctcggcgag
     1141 cggcgtgagg tcggtgaccg cggtggcgcg caggtcgaga cgctcgagcc gggtcgcacc
     1201 gcgcagcgcg cggatgtcgg tgagctgggt cgcgccgagc gccaaagtcc gcagctcggc
     1261 gagcgagccc agggtggaga ggtcgaccgg cgtgtgcgac aggttcaggg actcgagtgc
     1321 caggccgttc agcggcgaga cgtcccggac cccggtgctt tcgagctgca ggttgcgcag
     1381 acggccaagc ccgccgagcg atgcgaggtt ggacacaggg gtaccgctca gcgtcagctc
     1441 ctccagccgg cccagtccgg caagcggtgc gaggtcggac acgcgcgtgc catcgagggc
     1501 gagcacacgc agctcgacga gcctggcaag cggcgcgacg tcgctgacgc gcgtattcga
     1561 cagcgtcaag gtctccagtc cggtcaggcc ggcgagcgca gcgagatcct cgacctcgct
     1621 gccgtccagc cgcagctcgc gcagggcatg cagtgttcgc agcccggaca gatcgccgac
     1681 cggcgcgttg atcatccgga gctggcgcag cgcggccgcc cccgccagcg cgaccgtgga
     1741 ccgcacgcga gtgtccgtga ggtcgagtgc ctctgcctgc gtgatcgcgg gccgatctgc
     1801 gagcggggtg aggtcgctca ccggcgcacc acggagcacg agcctgcgga gcccgggcag
     1861 atcgcgcagc ggaccgagcg catcgacctg ggtgtgcgac acgtcgaggt cctcgagctc
     1921 ggtcaggccc gcaagcggcg cgagatcggc gactgcggtc gacgcgacgt cgaggctggt
     1981 cagcgtgcgc agaccggcca gcggcgccag ctcgcgcacc cggctgcgcg agacatcgag
     2041 gcggcgcagg tgagacagcg cggcgagcgg tctcaggtcg gacaccggcg cgcccggcag
     2101 ctcgagcacc tggagctgcc ggagcccggc gagcggcgac atgtccgtaa cccggggatc
     2161 agtcggttcg tgtggattgg tattgcgcat ccgcagctcg cggagctcgg tcagctggcg
     2221 cagcggcgcg aggtcctcga cgggcgtgtt gacgcacacg agccgcgttt catcgatagg
     2281 cacgtcgcgc tggcagaatc gaatggtcgc ggtcatggtc cggggctaca gcggcttgcg
     2341 atactctttg accaaccagt cgacgagcgc cttgtcatcg gctgcattgc cggtcagcgc
     2401 gccgtagctc ttgatcgagc cgtcgatcac gtcctggaca tagtccgcct tgaccttggg
     2461 cgcaaccggc gccttgggaa ccgcctgctt ctgcaccgcg ttctggatga tcgtcatggc
     2521 gtgggccgcg ccctctgaca gcacgatgtc gttcatgttg atcgtggacg gcgtgctctg
     2581 ctcgtggacc gtcagcttct cgagcttcga gatctcgagg atgcgggcct tcttgctggc
     2641 gaacgccccc gggttgccgg cttcgacctc tctgcggatc gttcgcagga acgagtgcac
     2701 atccaccgcc ttgtcccacg ccttccggag gtactcgctg gcttcgcgcc gtacctcgtc
     2761 gtcgaactcg accttgccgc cgcccttctt gacacccggc ttgaaggtaa cgccggcata
     2821 cagcgacgta ccgagctccc gcgccggcag ctcctcgtag tgcgcggcgt tgttgacctt
     2881 ctcatcctcc gacatcgcct cgaacccgtc gctaccgtaa tagccgtgat cgtcgacggt
     2941 gcccttcgcg aggtggcagc actcgtggat gatggtgatc ttcgccgcgt tggggtcctg
     3001 gacttcggca accgcgcgct cgaggtggat gtgctggctc ccgaaatccg cccgccgagg
     3061 ccgacctgct cgtcgtcgcg gttgtagtcg gtgtcgatcg cggtgtccat gttggtcttc
     3121 acgttgccga tcgcggcctt ggccttggca tagactgcct tcgcgaccgc gtgcttggtg
     3181 ccgaacacct cctggagccc ggcggtgttc gccatcgcgt ggtcgatcaa cttgatggcg
     3241 tcgtccatca ccgccttgag cttcggcttg tcggtggcgc tgacctgggt ctcggcatcg
     3301 accaccgtcc tgtagacctt gtcgcggtcc tgacgtcaac gaccttctcg aactcggctt
     3361 gatcgaactt tccagcgccc gggaagatcc gcgccatgac gtcggcgatc ggatccttgg
     3421 tcttgagctg gccctccttg gccatccgcg tgagcgcgag cgcgatcgag tccttcagct
     3481 tggcctcggg taccttcccg gcggcgagga tcgcgctgat gtcggttgac ttcggagcgc
     3541 ggaacttgac attgctggtc ggctgtcgcg cggcgcgctc ggcctgaagc tgaccggaga
     3601 gatcgccggt gctcatcatt ccttggccat ggccgccgag gtccgcgctg aaggcgtcgg
     3661 tgagcgcgcg cttccctggt gatgccggcg cgatcgactc ggccagccgc gttgtcttgc
     3721 ccggttcgcg gccctctcgt cccgctatgg tcatcgtcct gcccctgaga ccggcaccga
     3781 gccggcgacc acgaatgtac tacggtcagg gcggttccca agcgctaccc ggtcgcgagg
     3841 cggcggcgca cgatgcggcc cgtgtgtccg gtccgcgcat ggcgtcgaat gacctcgaaa
     3901 ctgttcggaa tccagcagaa cggcgtttcc ctgggaaccc cgagttgccg gcgaacgtaa
     3961 gctggcgatc gatgggcgga gtcgcaggga aacgcagcag cgatcacgcc gggccggcca
     4021 ccgcgagcca cgacgtacac gcggcgccag gcaagaggtc gaggtccgca agcggatcaa
     4081 ggccgacgac ctcgacatcg cgcaggagct catggacgtc agcatgaagg agctgttcgc
     4141 gaccgccgac aagggcgtca agaaggagcc gttccaggcc cgccgcgacg cgctgcaagc
     4201 cgagatcaag gccgccagga aggccaagaa gccgtgaccg cgcggcgcgc cgtgctcgtg
     4261 gcgctcatcg ccgcagcctg cggtgcgcgg cccgctccgt ctcccggtgg gagcccgggc
     4321 accatgatcc acgatgacgc gaccccgcgc acgctgtaca ccgtcgagct caccctcacc
     4381 gcggatcccg ccatcgccgg gcgcgccacg ctgcgcatcg cctcgcgcgg ccgcgatccg
     4441 gtgacggtcg cgatcccgga gcgccctggt ggcgagcgcg ccgcgatcgc ttacacgatc
     4501 ctcggctcgt gccgtcttgc aggtgtcgac ccgcgcgagt acctcgccga cgtcctgccg
     4561 cggctgaccg ggcgcattcg cctcgtggac ctgccggcgc tgctcc
//
LOCUS       DASVOR010002146         2937 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_402085,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002146 DASVOR010000000
VERSION     DASVOR010002146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2937
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402085"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..>2937
                     /locus_tag="VF516_39825"
     CDS             133..>2937
                     /locus_tag="VF516_39825"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8113968.1"
                     /translation="MSISEPFIRRPVATTLLMFGLMLAGLTGYFRLPVAALPQVDYPT
                     IVVATQLPGASAETMASAVTTPLERQFGQMPSLAQMTSVSSFGSSQITLQFTLDRDID
                     AAQQDVQAAINAASNLLPRTLPAPPTYSKSNPADQPILTLSVSSDTLSLAEVDDFADS
                     VLAQKISQVSGVGLVTIGGGQKPAVRVQVDPVALAGTGLTIEDVRLAIAAANVNQPKG
                     NLDGPRQDWALATNDQLASAASFRPLIIAYKNGAPVRLSEVANVVDGVENAQLAGWAG
                     TTEAGRAASQPTAPASRSHAAAEPVASGGQLRRAIIVNVQRQPGANIIEVTDNIKKLL
                     PQLRASMPQAIDVEILADRTETVRASVDDVQFTLLLTIGLVVAVIYLFLGSIRATFIP
                     GVAVPLSLIGTFGVMKLWGFSLDNLSLMALTISTGFVVDDAIVMVENVSRFIEHGDRP
                     FDAAIKGAKQIGFTIVSLTVSLVAVLIPLLFMGGLVGRLFNEFAVTLAIAIVLSAILS
                     LTLTAMMCAWILRPHAEQHPGAIARGFERGFAALGRFYDRTLQVVLRHQPTTLLVTVA
                     TLAVTVGLAFHVPKGFFPVEDTGMIVGVTEAAPDVSFGTMMALQQRAADAVLEEPAVA
                     TVSSFIGADGTNPTQNSGRLSIALTPRDDRDADVTEVIARLQDRLSGVQGVAVYLQPV
                     QDLTVDARIARTQYQYTIEDPDPGELATWAPRMLDAMRKLPELRDVASDQQTAGLELH
                     LAVDRDSASRLGITAQALDDTLYDAFGQRIVSTTFTQLNQYRIILELRPEDRENIDSL
                     DRIYVRSGAGAAIPLSAFSKLETRSTALAIDHQGQFPSVTLSFNTASGVSLGEAIAAI
                     DAASARLDPPPAMRGEFQGAAAVFRESLASEPLLILAALITVYIVLGVLYESYIHPIT
                     ILSTLPSAGVGAILALELTGK"
BASE COUNT          422 a         1086 c          996 g          433 t
ORIGIN      
        1 ggcgcagcgg ccgcacccgg tgaccggcca ggcggactcg accgaaggga ggcgaggggc
       61 agcgcccccc gtgggatcga tgccacgcgg tccggcagcg gggccgaccg cgtcgaacca
      121 gcccgccgcg ccgtgagcat ctccgagccg ttcatccggc ggccggtcgc gaccacgctg
      181 ctgatgttcg gcctgatgct cgcgggcctg accggctact tcaggctgcc ggtcgccgcg
      241 ctgccccagg tcgactatcc gacgatcgtg gtggcgaccc agctgccggg cgcgtcggcc
      301 gagaccatgg cgtcggcggt gaccacgccg ctcgagcgcc agttcggcca gatgccgtcg
      361 ctcgcgcaga tgacctcggt gtcgagcttc ggcagctcgc agatcacgct gcagttcacg
      421 ctcgaccggg acatcgacgc cgcccagcag gacgtccagg ccgcgatcaa cgcggcgtcg
      481 aacctgctgc cgcggaccct gccggcgccg ccgacctact ccaagagcaa ccccgccgac
      541 cagccgatcc tgaccctgtc ggtgtcgtcg gacaccctgt cgctcgccga ggtcgacgac
      601 ttcgccgact cggtgctggc ccagaagatc tcgcaggtgt ccggcgtcgg cctggtgacg
      661 atcggcggcg gccagaagcc ggccgtgcgg gtccaggtcg atccggtcgc gctcgccggc
      721 accggcctga cgatcgagga cgtccggctc gcgatcgccg ccgccaacgt caaccagccc
      781 aagggcaacc tcgacggccc gcgccaggac tgggcgctcg cgaccaacga ccagctcgcc
      841 agcgcggcga gcttccggcc gctgatcatc gcgtacaaga acggcgcgcc ggtccggctg
      901 tccgaggtcg ccaacgtggt cgacggtgtc gagaacgccc agctcgccgg ctgggccggg
      961 acgaccgagg ccggacgcgc ggcgagccag ccgaccgccc cggcttccag gagccacgca
     1021 gcggcggagc ccgtcgcctc gggcggccag ctgcggcgcg cgatcatcgt caacgtccag
     1081 cgccagcccg gcgccaacat catcgaggtc accgacaaca tcaagaagct gctgccccag
     1141 ctccgcgcgt cgatgccgca ggcgatcgac gtcgagatcc tcgccgaccg caccgagacc
     1201 gtgcgggcct cggtcgatga cgtccagttc accctgctgc tgacgatcgg cctcgtggtt
     1261 gccgtgatct acctgttcct cggcagcatc cgcgcgacgt tcatcccggg cgtcgcggtg
     1321 ccgctgtcgc tgatcggcac gttcggcgtg atgaagctgt gggggttctc gctcgacaac
     1381 ctgtcgctga tggcgctcac gatctcgacc gggttcgtgg tcgacgacgc gatcgtcatg
     1441 gtcgagaacg tgtcgcggtt catcgagcac ggcgaccggc cgttcgacgc cgcgatcaag
     1501 ggcgccaagc agatcgggtt cacgatcgtg tcgctgaccg tgtcgctggt cgcggtgctg
     1561 atcccgctct tgttcatggg cggcctggtc ggccggctgt tcaacgagtt cgcggtgacg
     1621 ctggcgatcg cgatcgtgct gtcggcgatc ctgtcgctga ccctgaccgc gatgatgtgc
     1681 gcctggatcc tgaggccgca cgccgagcag cacccgggtg cgatcgcgcg cggcttcgag
     1741 cgagggttcg ccgcgctcgg ccggttctac gaccgcacgc tgcaggtcgt gctgcgccac
     1801 cagccgacca cgctgctggt cacggttgcg accctggcgg tcaccgtcgg cctcgcgttc
     1861 cacgtcccca aggggttctt cccggtcgag gacaccggga tgatcgtcgg ggtcaccgag
     1921 gcggcgcccg acgtgtcgtt cggcacgatg atggcgctgc agcagcgcgc ggccgatgcg
     1981 gtgctcgagg aacccgccgt cgccaccgtg tcgtcgttca tcggcgccga cggcaccaac
     2041 ccgacccaga acagcggccg gctgtcgatc gcgctgaccc cgcgcgacga ccgcgacgcc
     2101 gacgtcaccg aggtgatcgc gcggctgcag gaccggctca gcggcgttca gggcgtcgcg
     2161 gtgtacctcc agccggtcca ggacctcacc gtcgacgcgc ggatcgcccg cacccagtac
     2221 cagtacacga tcgaggatcc ggaccccggc gagctcgcaa cctgggcgcc gcgcatgctc
     2281 gacgcgatgc gcaagctccc cgagctgcgc gacgtcgcgt ccgaccagca gaccgcgggg
     2341 ctcgagctcc acctcgcggt cgatcgcgac agcgcgtcgc ggctcgggat caccgcgcag
     2401 gcgctcgacg acacgctgta cgacgcgttc ggccagcgca tcgtgtcgac cacgttcacc
     2461 cagctcaacc agtaccggat catcctcgag ctccggcccg aggaccgcga gaacatcgac
     2521 tcgctcgacc ggatctacgt gcggtcggga gccggcgccg cgatcccgct gtcggcgttc
     2581 tccaagctgg agacccgcag caccgcgctt gcgatcgacc accagggcca gttcccgtcg
     2641 gtcacgctgt cgttcaacac ggcgtccggg gtatcgctcg gcgaggcgat cgccgcgatc
     2701 gacgccgcct cggccaggct cgacccgccg ccggcgatgc gtggcgagtt ccagggcgcg
     2761 gccgcggtgt tccgcgagtc gctggcgtcc gagcccctgc tgatcctcgc cgcgctgatc
     2821 accgtgtaca tcgtgctcgg cgtgctgtac gagagctaca tccacccgat cacgatcctg
     2881 tcgaccctgc cgtcggcggg cgtcggcgcg atcctcgcgc tcgagctgac gggcaag
//
LOCUS       DASVOR010002147         2071 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_24265,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002147 DASVOR010000000
VERSION     DASVOR010002147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24265"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VF516_39830"
     CDS             complement(<1..1503)
                     /locus_tag="VF516_39830"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8113969.1"
                     /translation="MATHREDRTEHHSSKPAGVSATVETSDEVRVTTPAKVCGMGHRM
                     KRKEDPRFIQGKGRYVDDVKLPNMVYMDIVRSPYAHAKILKIDASAALATPGVLAVIT
                     GEDLKKAGNLHFMPTFMSDTQMVLPIEKVVYYAQEVCVVIATERYIAADAIEKVVVDY
                     EPLEPMIDPHEALTDKVIVREDKPTKTNHIWHWEAGDKAATDAVFASAATVVEEKIYL
                     PRIHVASIETCGMVANYDKARGHMQIYMTSQAPHAVRTVFALVSGLPEHMIQIISPDI
                     GGGFGGKVPVYPGYVCCAVASLVLGRPVKWIEDRSENLQADSFARDYHITARLAADKD
                     GKIVGLQVQTLSDCGAADAAANPSKFPAGLYSICTGSYDMKAAHIAVDGVYTNKPPGG
                     VAYRCSFRVTEAVHMIERMVDILAHQLGKDPADFRLQNFIRADQFPYKSPTGWEYDSG
                     NYKAALEKAMAMIDYRALRAEQLEKRKKGQLMGIGISSFTEVVGAGPSKDY"
     gene            complement(1507..1992)
                     /locus_tag="VF516_39835"
     CDS             complement(1507..1992)
                     /locus_tag="VF516_39835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8113970.1"
                     /translation="MSQHEITVTINGEKQTRTVEARLLLVHFIRETLGLTGTHIGCDT
                     TSCGACTISLDGEVVKSCTVFAVQADGATIETVEGLAAGGTLHPLQEGFWERHGLQCG
                     FCTPGMLMSARALLAKNPHPTEDEIRHGISGNLCRCTGYNKIVEAIQYAADKLGAAKG
                     A"
BASE COUNT          280 a          673 c          723 g          395 t
ORIGIN      
        1 gtagtccttg gacgggccgg cgccgacgac ctcggtgaag ctcgagatcc cgatgcccat
       61 cagctggccc ttcttgcgct tctcgagctg ctcggcgcgc agcgcccggt agtcgatcat
      121 cgccatcgcc ttctcgagcg cggccttgta gttgccgctg tcgtactccc agccggtcgg
      181 cgacttgtac gggaactgat cggcgcggat gaagttctgg agccggaagt cggccggatc
      241 cttgccgagc tgatgggcga ggatgtcgac catgcgctcg atcatgtgga cggcctcggt
      301 gacccggaac gagcagcggt aggcgacgcc gcccggcggc ttgttggtgt acacgccgtc
      361 gacggcgatg tgcgccgcct tcatgtcgta cgagccggtg cagatgctgt acaggccggc
      421 cgggaacttc gatgggttgg cggcggcgtc ggccgcgccg cagtccgaca gcgtctggac
      481 ctggaggccg acgatcttgc cgtccttgtc cgccgcgagc cgtgcggtga tgtggtaatc
      541 gcgcgcgaag ctgtcggcct gcaggttctc gctgcgatcc tcgatccact tgaccggccg
      601 gccgagcacg aggctggcga ccgcgcagca cacgtagccg ggatagaccg ggaccttgcc
      661 gccgaacccg ccgccgatgt cgggcgagat gatctggatc atgtgctcgg gcagccccga
      721 gaccagcgcg aacaccgtgc ggacggcgtg cggcgcctgc gacgtcatgt agatctgcat
      781 gtggccgcgc gccttgtcgt agttggcgac catgccgcag gtctcgatcg acgccacgtg
      841 gatccgcggg aggtagatct tctcctcgac gaccgtggcg gcgctggcga acaccgcgtc
      901 ggtcgccgcc ttgtcaccgg cctcccagtg ccagatgtgg ttggtcttcg tgggcttgtc
      961 ctcgcgcacg atcaccttgt cggtcagcgc ctcgtgcgga tcgatcatcg gctcgagcgg
     1021 ctcgtagtcg acgacgacct tctcgatcgc gtcggcggcg atgtagcgct cggtggcgat
     1081 caccacacag acctcctgtg cgtaatacac caccttctcg atcggcaata ccatctgggt
     1141 atcggacatg aaggtcggca tgaagtgcag attgccggcc ttcttgaggt cctcgccggt
     1201 gatcaccgcg agcacgccgg gggtggcgag cgcggcgctg gcgtcgatct tgaggatctt
     1261 ggcgtgcgcg tacgggctgc gcacgatgtc catgtagacc atgttgggca gcttgacgtc
     1321 gtcgacgtag cggcccttgc cctggatgaa ccgcgggtcc tccttgcgct tcatccggtg
     1381 gcccatgccg cacaccttgg ccggcgtggt gacccggacc tcgtcgctgg tctccacggt
     1441 ggccgagacg ccggccggct tggacgagtg gtgctcggtg cgatcttcgc gatgagtcgc
     1501 catggctcag gctccctttg cggcgccgag cttgtcggcg gcgtactgga tggcttcgac
     1561 gatcttgttg tagccggtgc atcggcacag gttccccgag atcccgtggc ggatctcgtc
     1621 ctcggtcggg tgcgggttct tcgcgagcag cgcgcgcgcg ctcatcagca tgccgggggt
     1681 gcagaacccg cactgcaggc cgtggcgctc ccagaacccc tcctgcagcg ggtgcagcgt
     1741 gccgccggcg gccaggccct cgacggtctc gatcgtcgcg ccgtcggcct ggaccgcgaa
     1801 cacggtgcag ctcttgacca cctcgccgtc gaggctgatc gtgcacgcgc cgcagctggt
     1861 ggtgtcgcag ccgatgtgcg tgccggtcag gccgagcgtc tcgcggatga agtggacgag
     1921 cagcaggcgg gcctcgaccg tgcgggtctg cttctcgccg ttgatcgtga cggtgatctc
     1981 gtgctggctc atggacggcc tccctgtgcg cgctcgatgg cgcggcgcac ggcgcgcttg
     2041 gtcaggaccc ggaccagatc gcgcttgtag t
//
LOCUS       DASVOR010002148         5034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_227674,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002148 DASVOR010000000
VERSION     DASVOR010002148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5034
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227674"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            259..4020
                     /locus_tag="VF516_39840"
     CDS             259..4020
                     /locus_tag="VF516_39840"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX8113971.1"
                     /translation="MAPHGHDTQLSGPGQDTAPGPRPAIVGIGAAAGALEALEAVTRR
                     LTSCGLAFVIMERVARKRPALAQFLARHAELPVITAADGMSVEADHIYVAPPGAELAI
                     RHGVLRLEAVPPGKASPRPIDAMLCTLAADLGNRAIGVILSGAGDDGTVGLQAIKEAD
                     GITFIQDPSTARRPDMPRSARHAGVADFVLAAEDIAGELIWLSRHPYIAARRQQRLDD
                     DTRNRLFLLLRRGFGVDFSGYRPVAIDRRIHRRMVLHKFTRAEDYLRQVQTTSAELGA
                     LYGDLLFSATGFFRDPAQFDALTSVVLPRLFDQRTVATPLRIWVPGCGTGEEVYSIAI
                     CLLEYLGDRAVDHEIQIFATDIDAGAIARARQAIYPPSIARQLSPERLQQFFWPSDSG
                     YQVQRAVRDLVVLAHHNLGEDPPFSRIDLVSCCHVLGALQPTRQHKVLRDLHFALNPD
                     GFLLLGADESLGEPSELFAPVDRQVQLYTKQVLATTAEGERFERRRVRQDAIALNREL
                     EAENQSLREAQSQLEESRGRYVDLYDFAPVAYCTFDRDGVVLEINLTGAALLGEDRVR
                     ILGRPFRALVRLDDPDALLVHIRAALATPQPTTGEIAFLTARGPCIARLISIPARDRR
                     GPATTCRTAMLDITRQRLAEREAGAVHASEQGLRIRIERLEHASAQVTAALAAPASSD
                     LRPLLQLIVDHARNVVEAEFAGLGIGGSQWRQFDPWVVSGVPAHHMASLGHPPRSVGL
                     LGAVTHASRPIRLRDVGDHSSFSGLPLHHPALTSFLGVPIRYLGERRASLYLANKRGG
                     AEFTEDDQLAIEMFAERVGVALEIARLRQIEVREHTRLELLAGAGPLLAEPIEYETTL
                     DAVARLVVPVMADLCAVDLVEDDGAMTKAAASHPEPDIQHELDRWLGTTGRDELPADL
                     RAAIETAQPQLRDGASEPLPGDAPEAGYRRLLDAIGVTCSIVAPLVVRGRVVGALRLA
                     MAGSGRRYVPHDLELARELAHHAALAIERARLYREAQVAIGTRDDLFAFVTHDLHNYL
                     ATIRVSAAALTAQHGPEQRPNGHKQVELISRTVAHMARLIEGLRDATMIESGQFTVAT
                     APQDVAALVGEAVETLAPQAEAKAQRLTAQLDDGLPAVACDRDRVLQVLVNLVGNAIK
                     FTPRRGDIRIAAARIDGAVRLSVSDTGLGIPEAQLARVFDRYWRGAGSGHGAGLGLFI
                     ARGSVEAHGGRIWVASTPGSGSAFHFTLPLAPVAARDAVRDAHAARPPS"
     gene            4292..4930
                     /locus_tag="VF516_39845"
     CDS             4292..4930
                     /locus_tag="VF516_39845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8113972.1"
                     /translation="MSTLTRVFLIDDHPLFREGLAGAIQAEPDFEVVGQAATLDELRA
                     LAPHIEPDVAIVDLLMPSVSGIGVTRELCELLPRCRVLGLSAVVDAAAIAEMLRAGAS
                     GFALKTQPAPDILDAVRRTAAGDSYLPPGVSREAVDAELAGGAPPSLAQLTKREREIF
                     ELMIRGYTNPEIASRLFISLRTVETHRHRIVKKVCARSIVEMQRLAARYGRF"
BASE COUNT          680 a         1889 c         1779 g          686 t
ORIGIN      
        1 ccccgccggc cgcgccgccg cccgacgccg gcccgccgac cggtggcgtg ctgctgtgat
       61 gggggatcct gacgcgccct gatcgcagcc acctgcagga ccgatgcgtc tggatcgcgc
      121 ctgcgacctc acaatgccgc acctgccatc gccgccggag cggccttgaa attccaagga
      181 aatctcgcta tttctaagct ggcctcgcgg gcgttggacc gtcccccagc tgtaaacgaa
      241 ggagtcgctc accgagtcat ggcgccgcac gggcacgata cgcagctgtc cggaccgggg
      301 caggacaccg ccccgggacc gcggcccgcg atcgtcggca tcggcgcggc ggcgggcgcg
      361 ctggaggcgc tggaggcggt gacccggcgg ctgacctcct gcggtctggc gttcgtgatc
      421 atggagcgcg tcgcgcgcaa gcgccctgcg ctggcccagt tcctcgcgcg ccacgccgag
      481 ctgccggtca tcaccgccgc cgacggcatg tcggtcgagg ccgaccacat ctacgtcgcg
      541 ccgcccggcg ccgagctcgc gatccggcac ggcgtgctgc gactcgaggc ggtgccgccg
      601 ggaaaggcca gcccgcggcc gatcgacgcg atgctgtgca cgctcgccgc cgacctcggc
      661 aaccgcgcga tcggcgtgat cctgtcgggt gccggcgacg acgggacggt cgggctgcag
      721 gcgatcaagg aagccgacgg gatcacgttc atccaggatc cgtcgacggc gcggcggccg
      781 gacatgccgc gcagcgcgcg ccacgccggc gtcgccgact tcgtgctcgc cgccgaggac
      841 atcgccggtg agctgatctg gctgtcccgc cacccgtaca tcgccgcccg gcgccagcag
      901 cggctcgacg acgacacccg caaccggctg ttcctcctgc tgcgccgcgg gttcggggtc
      961 gacttcagcg gctaccggcc ggtggcgatc gaccgccgga tccaccggcg gatggtgctg
     1021 cacaagttca cgcgcgccga ggactacctg cgccaggtcc agaccaccag cgcggagctc
     1081 ggcgcgctgt acggtgacct gctgttcagc gccaccgggt tcttccgcga tccggcgcag
     1141 ttcgacgccc tgacctccgt ggtgctgccc cggctgttcg accagcgcac cgtcgcgacg
     1201 ccgctccgga tctgggtccc gggctgcggc accggcgagg aggtgtactc gatcgcgatc
     1261 tgcctgctcg agtacctcgg cgaccgcgcc gtcgaccacg agatccagat ctttgcgacc
     1321 gacatcgacg ccggggcgat cgctcgcgcg cgccaggcga tctacccgcc gtcgatcgcg
     1381 cgccagctct cgccggagcg cctgcagcag ttcttctggc cgtcggacag cggctaccag
     1441 gtccagcgcg ccgtccgcga cctggtggtg ctcgcccatc acaacctcgg cgaggacccg
     1501 ccgttctcga ggatcgacct ggtgagctgc tgccacgtgc tcggcgccct gcagccgacg
     1561 cggcagcaca aggtgctccg cgacctccac ttcgcgctga acccggacgg cttcctcctg
     1621 ctcggcgccg acgagtcgct gggcgagccg tccgagctgt tcgcgccggt cgatcgccag
     1681 gtccagctgt acaccaagca ggtcttggcg accaccgccg agggcgagcg cttcgagcgc
     1741 aggcgcgtac ggcaggatgc gatagccctc aatcgcgagc tcgaggccga gaaccagtcg
     1801 ctgcgcgagg cgcagagcca gctcgaggag tcgcgcggcc gctacgtcga tctctacgac
     1861 ttcgcgccgg tcgcgtactg cacgttcgat cgcgacggcg tggtgctgga gatcaacctg
     1921 accggcgcgg cgctgctcgg cgaggaccgc gtgcggatcc tgggccggcc gttccgcgcg
     1981 ctggtccggc tcgacgatcc cgatgcgctg ctcgtccaca tccgcgccgc gctggccacg
     2041 ccgcagccca ccaccggcga gatcgcgttc ttgacggcac gcggcccgtg catcgcacgg
     2101 ctgatcagca tcccggcgcg cgaccggcgc ggaccggcga cgacctgccg caccgcgatg
     2161 ctcgacatca cgcgacagcg gctcgccgag cgcgaggccg gcgcggtgca cgcctcggag
     2221 caggggctcc ggatccggat cgagcggctg gagcatgcct cggcccaggt gaccgcggcg
     2281 ctggcggccc cggcgtcctc ggacctccgc ccgctgctcc agctgatcgt cgatcatgcg
     2341 cgcaacgtgg tcgaggcgga gttcgccggc ctcgggatcg gcggcagcca gtggcgccag
     2401 ttcgacccgt gggtggtcag cggcgtgccc gcccatcaca tggcgagcct cggccaccca
     2461 ccccggtcgg tcggcctgct cggcgcggtc acccacgcga gccggccgat ccggctgcgc
     2521 gacgtcggcg accactcgtc gttctccggc ctgccgctgc accacccggc gctgaccagc
     2581 ttcctcggcg tgccgatccg gtacctcgga gagcgtcgcg ccagcctgta cctcgcgaac
     2641 aagcgcgggg gcgcggagtt caccgaggac gaccagctgg cgatcgagat gttcgccgag
     2701 cgggtcggcg tcgcgctgga gatcgcccgg ctgcgccaga tcgaggtccg cgagcacacc
     2761 cggctcgagc tcctggccgg ggccgggccg ctgctcgccg agccgatcga gtacgagacc
     2821 acgctcgacg ccgtcgcgcg gctggtcgtc ccggtgatgg ccgacctgtg cgcggtcgac
     2881 ctggtcgagg acgacggcgc gatgaccaag gcggccgcct ctcaccccga gccggacatc
     2941 cagcacgagc tcgaccgctg gctcggcacc accgggcgcg acgagctgcc cgccgatctg
     3001 cgcgccgcga tcgagaccgc gcagccgcag ctgcgcgacg gcgcctccga gccgctgccc
     3061 ggcgacgcgc ccgaggccgg ctatcgcagg ctgctggacg cgatcggcgt gacctgctcg
     3121 atcgtcgcac cgctggtcgt gcggggccgc gtggtcggcg cgctgcgcct ggcgatggcc
     3181 ggatcgggac ggcgctacgt cccgcacgat ctcgagctcg cccgggagct cgcgcaccac
     3241 gccgcgctcg cgatcgagcg cgcccggctg taccgcgagg cgcaggtcgc gatcggcacc
     3301 cgggacgacc tgttcgcgtt cgtcacccac gacctgcaca actacctcgc gacgatccgc
     3361 gtgagcgccg ccgcgctgac cgcgcagcac ggccccgagc agcgcccgaa cgggcacaag
     3421 caggtcgagc tgatcagccg cacggtggcc cacatggcgc ggctgatcga gggcctgcgc
     3481 gacgcgacga tgatcgagtc cgggcagttc acggtcgcga cggcgcccca ggacgtcgcg
     3541 gcgctggtcg gcgaggcggt cgagaccctc gcgccgcagg ccgaggccaa ggcgcagcgg
     3601 ctcaccgccc agctcgacga cgggctgccc gccgtggcgt gcgaccgcga ccgcgtgctt
     3661 caggtgctcg tgaacctggt cggcaacgcg atcaagttca ccccgcggcg cggcgacatc
     3721 cggatcgcgg cggcgcgcat cgacggtgcg gtccggctgt cggtgtccga caccgggctc
     3781 gggatccccg aggcccagct cgcgcgcgtg ttcgaccgct actggcgagg cgccggcagc
     3841 ggccacggcg ccggcctcgg gctgttcatc gccaggggga gcgtcgaagc ccacggcggc
     3901 cggatctggg tcgcgagtac gccgggctcc ggcagcgcct tccacttcac gctgccgctc
     3961 gcgccggtgg cggcgcgtga cgccgtgcgc gacgcgcacg ctgcacgccc gcccagctga
     4021 tcgcggatcg ccccgcgcgc caccgcacac cgtcgggtga tcgcgccagt atgccgcacg
     4081 ggcgggcgca gccgcgcggc ccacctacgt gatttcccgt atcatccgca caccgcctgc
     4141 gacaagacgc gacagaagga ttccgaaaat gtagcggggg acagggcgtg cccccgagca
     4201 cgcctcggcg tgcgccggag gtgaggcatg accagcccgc gggcgttgga cgacaagcaa
     4261 atcgcgctgc taggctcctc tcgctcacgc tatgtcgacc ctgacgagag tgttcctgat
     4321 cgatgaccat ccgctgtttc gcgagggcct ggcgggtgcg atccaggcgg agccggattt
     4381 cgaggtcgtc ggccaggccg cgaccctgga cgagctgcgc gcgctcgcgc ctcatatcga
     4441 gccggacgtc gcgatcgtcg acctgctgat gccgtcggtc tcggggatcg gagtcacccg
     4501 cgagctgtgc gagctgctgc ccaggtgccg cgtgctcggg ctgtccgccg tcgtcgacgc
     4561 cgccgcgatc gccgagatgc tgcgtgccgg cgcgagcgga ttcgcgctga agacccagcc
     4621 cgcgcccgac atcctcgacg cggttcgccg caccgcggcc ggcgacagct acctcccacc
     4681 gggcgtgtcg cgcgaggccg tcgatgccga gctcgccggc ggcgccccgc cgtcgctcgc
     4741 gcagctgacc aagcgagagc gcgagatctt cgagctgatg atccgcggtt acaccaaccc
     4801 ggagatcgcg tcgcggctgt tcatctcgct gcgcaccgtg gagacccacc gccaccggat
     4861 cgtgaagaag gtgtgcgcgc gctcgatcgt cgagatgcag cggctcgcgg cgcgctacgg
     4921 ccggttctag gatcgcggat cgcggtgctg caatccgagc cgcgggccca ccgcgcgcgc
     4981 ggcggtgcgc tgtagctggc ggtctgcgac gccggcagcg cgacgtggtc gcgg
//
LOCUS       DASVOR010002149         9810 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_343850,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002149 DASVOR010000000
VERSION     DASVOR010002149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9810
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343850"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF516_39850"
     CDS             complement(<1..612)
                     /locus_tag="VF516_39850"
                     /inference="COORDINATES: protein motif:HMM:TIGR04025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEX8113973.1"
                     /translation="MSAPVREITSATELRELLGEPTPRSLVKERPRLHARDRDWLAAS
                     PFCLVATADAEGNCDVSPKGDPPGFTVVLDDSTIAIPERIGNRRADGFHNILRNPHVG
                     LIYLIPGRNDTLRINGRATLIRDAPWFDQLIVKGHRPQLAVVVAIDTIFFHCAKAFMR
                     SALWQPETWKSDALPRQGAIVKSLHPDLPGTVEEIEADYAARCA"
     gene            843..1484
                     /locus_tag="VF516_39855"
     CDS             843..1484
                     /locus_tag="VF516_39855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113974.1"
                     /translation="MSKLVPLGENHLRRAVSEYVEHYHRERNHPGVGNRLLTPAEPAV
                     RPANANAPVERRERLGGLLSGPALDRRKATAYAARMRVRATLLVLLTACAQGGAVAPA
                     PQQPEPKPQERADPADQRLRAKTKVKLVLYAFEAYPPWSAAHPDKACPEKLAELNEYI
                     NSSDTNDAWGRPLKMLCGAALPAGAKGIGVLSVGEDGKEGTDDDIKSWELNGH"
     gene            complement(1508..1786)
                     /locus_tag="VF516_39860"
     CDS             complement(1508..1786)
                     /locus_tag="VF516_39860"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HEX8113975.1"
                     /translation="MRTAAVTRSSAASSEPLAWPTRPPGSRLRPLRTSISAGSWPRDL
                     VLDPFFGSGTTGQVAEKHSRRWIGFDLGYEELAKERTAQRSLALETSR"
     gene            1801..3015
                     /locus_tag="VF516_39865"
     CDS             1801..3015
                     /locus_tag="VF516_39865"
                     /inference="COORDINATES: protein motif:HMM:NF016915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8113976.1"
                     /translation="MPDDVSRKAARNHADDLRGASRLAIDATRSVTGLVEAMHTTIAS
                     GPAILGRPLAAPAQLANRLVYGTIRGVTELVGSAIEIALAPLAPLLGASMPSAEREAL
                     LAALNGVVGDHLSDSGNPLAIAMQLRHGGVPLELAPEALRGALPHAGRKLVVLVHGLC
                     MTDHQWLRRGHDHGAALARDLGYTPVYALYNSGLHISINGRALAAQLAQLVAAWPVPL
                     DELVLIGHSMGGLVARSACHAGDEAAHAWRGRLRALITIGSPHHGAPLERGGHGIDRA
                     LEISPYVAPFRRLGRIRSAGITDLRFGNVLDEHWQGRDRFGHHGDTRHSLELPAGVAC
                     FAIAGTLATAAADRLRGDGLVPVASALGVHPTPALALRFPDVHRWIALGTAHNELLAA
                     PGVYATIRSWLD"
     gene            3229..3699
                     /locus_tag="VF516_39870"
     CDS             3229..3699
                     /locus_tag="VF516_39870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113977.1"
                     /translation="MSEEIEETSNPRCWKKAGWIARVVKNENDDGWAVEMTRIGESEP
                     SLVSPWTMGRDKVNPKPLDHAGFATLVKGATEVMARHAQAARARLHRSIYCQSETGAR
                     LRASFDIQEDEDDPHAILVVTDEQTGETLRSGRASTGFKLNEKAVRQFLRTGDA"
     gene            3964..4179
                     /locus_tag="VF516_39875"
     CDS             3964..4179
                     /locus_tag="VF516_39875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113978.1"
                     /translation="MSKLVPLGENHLRRAVSEYVEHYHRERNHQGMGNRLLTPAEPAV
                     RPANANAPVERRERLGGLLNFYYRRAA"
     gene            complement(4237..5145)
                     /locus_tag="VF516_39880"
     CDS             complement(4237..5145)
                     /locus_tag="VF516_39880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEX8113979.1"
                     /translation="MTSEPPVGSVDELIARLADHRYLAAPSLATVLYLGLRLGKPVLL
                     EGEAGVGKTEVAKVLAAALGRRLIRLQCYEGLDVASALYEWDFARQMLEIRLAEAAGP
                     VERGALATQLYSERFLLRRPLLDALTADPSGPPVLLIDELDRTDEPFEAFLLEILSDF
                     QVSIPELGTLRAPAPPITLLTSNRTREIHDALKRRCLYHWIDYPDAERELAILRARAP
                     EVPPRLASQIVSFVHALRASDLFKLPGVAETLDWGHALVALGCVELDPGAVEATLGVL
                     LKYQDDLARITGQPAQRLVEAARGAG"
     gene            complement(5142..5936)
                     /locus_tag="VF516_39885"
     CDS             complement(5142..5936)
                     /locus_tag="VF516_39885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HEX8113980.1"
                     /translation="MKDFAFHRPATIKEAVALLHAHANGKFLAGGQSLLPVLKLELAE
                     PTDLISLAHVKELHDIRHDGGNLAIGALVTHDQVHRSPEVKKAISALATLAGGIGDAQ
                     VRNRGTLGGSVAHADPAADYPAAIVGLGATVVTDRRKIAAEDFFTGLFATALAADELV
                     TAVHFPVPRRAAYAKFAHRASKYALVGVFVAETAGGVRVAVTGAGPKVFRVPSFEAAL
                     GKKLDAASLDGLTVPSTGFNGDAEASADYRAHLIGVMARRAVQALA"
     gene            complement(5938..8334)
                     /locus_tag="VF516_39890"
     CDS             complement(5938..8334)
                     /locus_tag="VF516_39890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEX8113981.1"
                     /translation="MTTNQGIGAAVKRKEDRRFLLGKGQYVDDIVVPDQTYAVFVRSM
                     YAHAKITGIDTAAAKAAPGVVGVFTGDDIAADGLGGLPCGWLIKNKDGSNMVEPPHPA
                     LAQGKVRHVGDPVAMVIATSKAAARAAAGLVDVNYDALPAIGLLADAFKPNAVLVHDE
                     AKGNVCYDWHIGDPAATDAAFAKAAHVVELDLTNNRLAPNPIEPRAVIGVYDVAADHH
                     TLYTTSQNPHVIRLLMGAFTLHVPEHKLRVVAPDVGGGFGSKIFHYAEELMVTWAAKK
                     VGRPVKWTADRSEAFMTDAHGRDHISHASLALDGNGKILGLKVSTLANLGAYLSTFGS
                     SVPTYLYATLLSGPYDMPAIYAEVKAAFTNTVPVDAYRGAGRPEAAYLLERLMDKAAR
                     QLGIDRIEFRRRNLIPADKFPYQTAVALQYDIGNYQATLDLALQQIDFANYDKRKAAS
                     AAKGKLRGFGISTYIEACGIAPSAIAGALGARAGLYEAATVRVNPTGSVTIFTGTHSH
                     GQGHETTFAQIVADKLGVTVDNVDVVHGDTDRILFGMGTYGSRSAAVGGSALIKALDK
                     VIDKGKKIAAHLLEASAGDIEFKDGNYRVAGTDKVKAFGEIAFTAYVPHNYPIETLEP
                     GLEESAYYDPKNFTFPGGCHLVEVEVDPETGVPEVVEVAIADDVGTIINPMIVDGQAH
                     GGLAQGIGQALYEEVRYDAEGQLTNGSFMDYCMPRAADLPNFKVGNHVTACTHNPIGV
                     KGVGECGAIGVPPAVINAIIDALTPLGVTDLSMPATPEKIWRAIQAAKAAPKAGSKGA
                     "
     gene            complement(8334..8807)
                     /locus_tag="VF516_39895"
     CDS             complement(8334..8807)
                     /locus_tag="VF516_39895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEX8113982.1"
                     /translation="MATIKLKVNRQAHIIECASTTLLVEALRDHLRLTGTHVGCDTSQ
                     CGACTVHVDGKSVKSCTMLAVEADGAEVTTIEGMASGGALHPMQAAFNEHHGLQCGFC
                     TPGMIMSATDLCKRVPDPSEHEIRHWLEGNLCRCTGYHNIVKAVRAGAAGMRGGK"
     gene            8926..>9810
                     /locus_tag="VF516_39900"
     CDS             8926..>9810
                     /locus_tag="VF516_39900"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8113983.1"
                     /translation="MAPDPAAWRRDFPALDAKVHGRRLAYLDSASTSLKPRAVIDAVV
                     QVFTHQAGNVHRGVHALSEAATEAYDAARDAIARFIGGRPDEIVLTGGTTEAINLIAH
                     SWGRAHVGPGDAVIATALEHHSNLVPWQVLCAERGAVLRIAPIDDRGRIDLAALAGLL
                     DERVRLVAVSQLSNVVGTIAPIAEIARLAHAAGAVVLVDGAQAVAHLPVDVAALGCDF
                     YAFSGHKLFAPTGTGVLWGRRALLAAMPAWQTGGEMVAAVDYERARLREPPHRFEAGT
                     PNIAGVIGLGAAIRYLAGI"
BASE COUNT         1415 a         3305 c         3640 g         1450 t
ORIGIN      
        1 ggcgcagcgc gcggcgtagt cggcttcgat ctcctcgacg gtccccggta ggtcggggtg
       61 cagcgacttg acgatcgcac cttgccgcgg cagcgcgtct gacttccagg tctccggctg
      121 ccacagcgcc gagcgcatga aggccttggc gcagtggaag aagatggtgt cgatcgcgac
      181 gacgacggcg agctgggggc gatggccctt gacgatcagc tgatcgaacc acggggcatc
      241 gcggatcagc gtcgcgcgcc cgttgatccg cagcgtgtcg ttgcggccgg ggatcaggta
      301 gatgagcccg acgtgcggat tgcgcaggat attatggaag ccatcggcgc gccggttgcc
      361 gatgcgctcg gggatcgcga tggtcgaatc gtcgaggacg acggtgaagc ccggcggatc
      421 gcccttgggc gagacatcgc agttgccctc ggcgtcggcg gtggcgacca ggcagaacgg
      481 cgacgccgcg agccagtcgc gatcgcgcgc atggaggcgc ggccgctcct tcaccagcga
      541 gcgcggggtt ggctcgccga gcagctcgcg aagctcggtc gccgacgtga tctcgcggac
      601 aggagcggac atgcgacata cgtagcgtag ccagcgggcg tcggctgcac agatcgcggg
      661 agcggcggcg gatcagacag ccaggcacgc gcctgggaca gtcagcgcgg gggctggttc
      721 ctggtcctct ggatggcggg ctggatcacc ccccgccagc ttgaggtcat cgagttcctg
      781 cgcgaggaga accacacggc tggtcggcgc gctggacaac ctcggccacc aagtccgagt
      841 gtttgagcaa gctggtgccg ctgggtgaga accacctgcg gcgagcggtg tcggagtacg
      901 tcgagcacta tcatcgcgag cgcaaccacc cgggcgtggg caaccggctg ctcacgccag
      961 ccgaaccggc ggtgcgacca gccaacgcga acgcgccggt cgagcgccgc gagcggctgg
     1021 gcggactgct cagcggcccc gcactggatc gccgcaaggc gaccgcatac gctgcccgca
     1081 tgagggtcag ggccacgttg ctcgtcttgc ttaccgcgtg tgctcagggc ggggcggtcg
     1141 ctcccgcccc gcaacagcca gagccgaagc cgcaggagcg tgccgatccg gccgaccagc
     1201 ggctccgagc caagacgaag gtgaagctcg tgctgtacgc cttcgaggcc tatccgccgt
     1261 ggtccgcggc gcaccccgac aaggcgtgcc cggaaaagct cgccgagctg aacgagtaca
     1321 tcaactcgag cgacacgaac gacgcctggg gccggccgct gaagatgctg tgcggcgctg
     1381 ccctgccggc cggcgccaag ggcatcggtg tgctgtcggt gggcgaggac ggcaaggagg
     1441 ggaccgacga cgacatcaag tcctgggagt tgaacgggca ctgagcgcgc gagctggtgt
     1501 gacgccgtca tcgcgatgtc tccagggcga gcgagcgctg cgcggtgcgc tccttcgcga
     1561 gctcctcgta gccgaggtcg aaaccgatcc agcgccggct gtgcttctcg gcgacctgtc
     1621 cggtcgtgcc gctgccgaag aacggatcga gcaccaggtc gcgcggccac gagccagccg
     1681 agatcgacgt ccggagaggg cgcagccggg atccgggagg acgagtgggc caggcgagag
     1741 gctcgctcga ggcggccgaa cttcgcgtca cggcagccgt gcgcatgaga agctggcggc
     1801 atgcctgatg acgtctcccg gaaggccgcg cgcaatcacg ccgacgatct acgcggcgcg
     1861 agccggctcg cgatcgatgc gacccgcagc gtgaccggcc tggtcgaggc gatgcacacg
     1921 acgatcgcga gcggcccggc gatcctcggc cggccgctcg ccgcgcccgc gcagctcgcc
     1981 aaccggctgg tctacgggac catccgaggt gtgaccgagc tcgtcggcag cgccatcgag
     2041 atcgcgctcg ccccgctggc cccgctgctt ggcgcgagca tgccgagcgc cgagcgcgag
     2101 gcgctgctcg ccgccctcaa cggggtggtc ggcgatcacc tgagcgacag cggcaacccg
     2161 ctcgcgatcg cgatgcagct gcgccacggc ggcgtgccgc tggagctcgc gcccgaggcg
     2221 ctgcgcggcg cgctgccgca cgccggccgc aagctggtgg tgctggttca cggtctgtgc
     2281 atgaccgatc accagtggct gcgacgcggc cacgatcacg gcgccgcgct cgcccgcgac
     2341 ctcggctaca cgccggtcta cgcgctctac aacagcggac ttcacatctc gatcaacggg
     2401 cgggcgctcg cggcgcagct cgcgcagctc gtcgcagcgt ggccggtgcc gctcgacgag
     2461 ctggtgctca tcggccacag catgggcggc ctggtggcgc gcagcgcctg ccacgccggc
     2521 gacgaggccg ctcacgcctg gcgcgggcgg ctgcgcgcgc tgatcacgat cggctcgccg
     2581 caccacggcg caccgctcga acgcggcggc cacgggatcg accgagccct ggagatcagc
     2641 ccctacgtcg ccccgttccg ccgcctcggc cggatccgca gcgccgggat caccgacctg
     2701 cgattcggca acgtgctcga cgagcactgg caaggccggg accggttcgg ccaccacggc
     2761 gatactcggc attccctgga gctgcccgca ggcgtggcgt gcttcgcgat cgccgggacg
     2821 ctcgcgaccg ccgcggccga ccggctgcgc ggcgacggcc tggtcccggt ggcgagcgcg
     2881 ctcggtgtgc atcccacgcc ggcgctggcc ctccggtttc ccgacgtgca tcgctggatc
     2941 gcgctcggga ccgcgcacaa cgagctgctg gccgcacccg gagtctacgc gacgatccgg
     3001 tcctggctgg actagcccgg cggcgcgcac ccaggacgca agccgtcatc ggctccggat
     3061 ctgccgcgcc gggatctcga tcgacctgtt cggtcccatc gctccagagc cggcagaggc
     3121 accggcgctc gatctccccg ccggggcggc ctcgcacaag ccctgacgcc tccccgctgg
     3181 gcgccacggc tcgctcgcga gtgtcagccc ctcgagcgaa gctgccgcat gtccgaagaa
     3241 atcgaggaga cctccaaccc ccggtgctgg aagaaggcgg gctggatcgc cagggtggtc
     3301 aagaacgaga acgacgacgg ctgggcggtc gagatgacgc ggatcggcga atcggagccg
     3361 tcgctggtca gcccgtggac gatggggcgt gacaaggtca accccaagcc gctcgatcac
     3421 gcggggttcg cgacgctggt caagggcgcg accgaggtga tggcgcggca cgcacaggcg
     3481 gcgcgagcgc ggctgcatcg gtcgatctat tgccagagcg agaccggcgc gcggctacgc
     3541 gcgagcttcg acatccagga agacgaggat gatccccacg cgatcctggt ggtgaccgac
     3601 gagcagaccg gcgagacgct gcgctcgggt cgcgccagca cgggcttcaa gctcaacgag
     3661 aaggcggtgc ggcagtttct gcggactgga gatgcctgaa gcggcacaaa tacccgcatt
     3721 cgctcagttt atccgcgcat caagccgcac cgatggtcaa tagctcacgc ttggactcga
     3781 cgggcgtcgt gtgccgaaag tcgtccagcg ggagacgcga ggcatctccg cggcttgcca
     3841 ttgccggtct gccgccctga aggggtactg cgtatgggtg caaaccgcgg tcgtgtggtt
     3901 ggtccagctg cgcccgtacg cagtacccct tcagggcggc agaccaagat caagtccgag
     3961 tgtttgagca agctggtgcc gctgggtgag aaccacctgc ggcgagcggt gtcggagtac
     4021 gtcgagcact atcatcgcga gcgcaaccac cagggcatgg gcaaccggct gctcacgcca
     4081 gccgaaccgg cggtgcgacc agccaacgcg aacgcgccgg tcgagcgccg cgagcggctg
     4141 ggcggactgc tcaacttcta ctaccggcgc gccgcatgag ctcgcgttcg acttctggca
     4201 catgacgccc gacggcgcgt ccgctgggcg cggcggttac cccgcgccgc gggcggcctc
     4261 gaccaggcgc tgggccggct ggccggtgat ccgggccagg tcgtcctgat acttgagcaa
     4321 gacgcccagg gtcgcctcga cggcgcccgg gtcgagctcg acgcagccga gcgcgaccag
     4381 ggcgtggccc cagtcgaggg tctcggcgac gcccggcagc ttgaacaggt cgctcgcgcg
     4441 cagggcatgg acgaacgaga cgatctggga ggcgagccgc ggcgggacct cgggggcgcg
     4501 ggcgcgcagg atcgcgagct cgcgctcggc gtcggggtag tcgatccagt ggtagaggca
     4561 gcggcgcttg agcgcgtcgt ggatctcgcg ggtccggttc gaggtcagca gggtgatcgg
     4621 cggggccggc gcgcgcagcg tgccgagctc ggggatcgag acctggaagt cggacaggat
     4681 ctcgagcagg aaggcctcga acggctcgtc ggtgcggtcg agctcgtcga tcagcaggac
     4741 cggcgggccg gaggggtcgg cggtgagcgc gtcgagcagc gggcggcgga gcaggaaccg
     4801 ctcgctgtag agctgggtgg ccagcgcgcc gcgctcgacc gggccggccg cctcggcgag
     4861 ccggatctcg agcatctggc gcgcgaagtc ccactcgtag agcgccgagg cgacgtcgag
     4921 gccctcgtag cactgcaggc ggatcagccg gcggccgagc gcggcggcga ggaccttggc
     4981 gacctcggtc ttgccgacgc cggcctcgcc ctcgagcagc accggcttgc ccagccggag
     5041 gccgaggtac agcacggtgg ccaggctcgg cgcggcgagg tagcggtggt cggcgagccg
     5101 agcgatcagc tcgtcgaccg agccgaccgg cggctccgag gtcatgccag ggcctggacc
     5161 gcgcgccgcg ccatcacccc gatcaggtgg gcgcggtagt cggcgctggc ctcggcgtcg
     5221 ccgttgaagc cggtcgacgg cacggtgagg ccgtcgagcg aggcggcgtc cagcttcttg
     5281 ccgagcgcgg cctcgaacga cggcacgcgg aacaccttgg gcccggcgcc ggtgaccgcg
     5341 acgcggacgc cgccggcggt ctcggcgacg aacacgccga ccagcgcata cttcgacgcg
     5401 cggtgcgcga acttggcgta cgcggcgcgc ctgggcaccg ggaagtgcac cgcggtgacc
     5461 agctcgtcgg cggcgagcgc ggtggcgaac aggccggtga agaagtcctc ggcggcgatc
     5521 ttgcgccggt cggtgaccac ggtggcgccc agcccgacga ttgccgccgg gtagtcggcg
     5581 gccggatcgg cgtgggcgac cgagccgccg agcgtgccgc ggttgcggac ctgggcgtcg
     5641 ccgatgccgc cggcgagcgt ggccagcgcc gagatcgcct tcttgacctc gggcgagcgg
     5701 tggacctggt cgtgggtgac cagcgcgccg atcgcgaggt tgccgccgtc gtggcggatg
     5761 tcgtggagct ccttgacgtg ggcgagcgag atcagatcgg tcggctcggc gagctcgagc
     5821 ttgagcaccg gcaggaggct ctggccgccg gcgaggaact tgccgttggc gtgggcgtgg
     5881 agcagcgcga cggcttcctt gatcgtcgcg ggacggtgga atgcgaagtc cttcatgtca
     5941 ggctcctttg ctcccagcct tgggcgcagc cttggcggcc tggatcgccc gccagatctt
     6001 ctcgggggtg gccggcatcg agaggtcggt gacgccgagc ggggtcagcg cgtcgatgat
     6061 cgcgttgatc accgccggcg ggacgccgat cgcgccgcac tcgccgacgc ccttgacgcc
     6121 gatcgggttg tgggtgcagg ccgtgacgtg gttgccgacc ttgaagttcg gcaggtcggc
     6181 cgcgcgcggc atgcagtagt ccatgaacga gccgttggtc agctggccct cggcgtcgta
     6241 gcggacctcc tcgtacagcg cctggccgat gccctgggcg aggccgccgt gggcctggcc
     6301 gtcgacgatc atcgggttga tgatggtgcc gacgtcatcg gcgatcgcga cctcgacgac
     6361 ctcggggacg ccggtctcgg gatcgacctc gacctcgacc aggtggcagc cgccggggaa
     6421 cgtgaagttc ttggggtcgt agtacgccga ctcctcgagg ccgggctcga gggtctcgat
     6481 cgggtagttg tgcgggacgt aggcggtgaa cgcgatctcg ccgaacgcct tgaccttgtc
     6541 ggtgccggcc acgcggtagt tgccgtcctt gaactcgatg tcgccggcgg aggcctcgag
     6601 caggtgggcg gcgatcttct tgcccttgtc gatcaccttg tcgagcgcct tgatcagcgc
     6661 cgagccgccg accgcggccg accgcgagcc gtaggtgccc atgccgaaca ggatgcgatc
     6721 ggtgtcgccg tggacgacgt cgacgttgtc gaccgtgacg ccgagcttgt cggcgacgat
     6781 ctgggcgaac gtggtctcgt ggccctggcc gtgcgagtgg gtgccggtga agatcgtcac
     6841 cgagccggtc gggttgaccc gcacggtggc ggcctcgtac aggccggcgc gcgcaccgag
     6901 cgccccggcg atcgccgacg gcgcgatgcc gcaggcctcg atgtaggtcg agatcccgaa
     6961 gccgcgcagc ttgcccttgg cggcgctcgc cgccttgcgc ttgtcgtagt tcgcgaagtc
     7021 gatctgctgg agcgcgagat cgagcgtggc ctggtagttg ccgatgtcgt actgcagcgc
     7081 gaccgcggtc tggtagggga acttgtcggc ggggatcagg ttgcggcggc ggaactcgat
     7141 ccggtcgatg ccgagctggc gcgccgcctt gtccatcagc cgctcgagca ggtacgccgc
     7201 ctcggggcgg ccggcgccgc ggtaggcgtc gaccggcacg gtgttggtga acgccgcctt
     7261 gacctcggcg tagatcgccg gcatgtcgta cggtccggac agcagggtcg cgtagaggta
     7321 ggtcggcacc gacgagccga acgtcgacag gtacgcgccg aggttggcca gcgtgctgac
     7381 cttgaggccg aggatcttgc cgttgccgtc gagcgcgagg ctggcgtggc tgatgtgatc
     7441 gcggccgtgg gcgtcggtca tgaacgcctc gctgcgatcc gcggtccact tgaccggccg
     7501 gccgaccttc ttggccgccc aggtgaccat cagctcctcg gcgtagtgga agatcttcga
     7561 gccgaacccg ccgccgacgt cgggggcgac gacccgcagc ttgtgctcgg ggacgtgcag
     7621 cgtgaacgcg cccatcagca gccggatcac gtgcgggttc tgcgaggtgg tgtacagcgt
     7681 gtggtgatcg gccgcgacgt cgtagacgcc gatcacggcg cgcggctcga tcgggttggg
     7741 tgcgagccgg ttgttggtca ggtcgagctc gacgacgtgg gcggccttgg cgaacgccgc
     7801 gtcggtcgcg gccggatcgc cgatgtgcca gtcgtagcac acgttgccct tggcctcgtc
     7861 gtggaccagc accgcgttgg gcttgaacgc gtcggcgagc aggccgatcg ccggcagcgc
     7921 gtcgtagttg acgtcgacca gcccggcggc ggccctggcc gcggccttcg aggtcgcgat
     7981 caccatcgcg accggatcgc cgacgtggcg gaccttgccc tgggcgagcg cggggtgcgg
     8041 cggctcgacc atgttcgagc cgtccttgtt cttgatcagc cagccgcacg gcaggccgcc
     8101 gagcccgtcg gccgcgatgt cgtcgccggt gaacacgccg accacgccgg gggcggcctt
     8161 ggcggcggcg gtgtcgatgc cggtgatctt ggcgtgcgcg tacatcgagc gcacgaacac
     8221 ggcgtaggtc tggtccggca cgacgatgtc gtcgacgtac tggcccttgc cgagcaggaa
     8281 ccgacggtct tccttgcgct tgacggcggc gccgatgccc tggttggtcg tcatcactta
     8341 cctccgcgca tgccggccgc acccgcgcgc acagccttga cgatgttgtg gtagccggtg
     8401 caccggcaca ggttgccctc gagccagtgc cggatctcgt gctcgctggg atccggcacc
     8461 cgcttgcaca ggtcggtcgc gctcatgatc atgcccggcg tgcagaaccc gcactgcagg
     8521 ccgtggtgct cgttgaacgc ggcctgcatc gggtgcagcg cgccgccgct ggccatgccc
     8581 tcgatggtcg tgacctcggc gccgtcggcc tcgaccgcga gcatcgtgca cgacttgacc
     8641 gacttgccgt cgacgtggac cgtgcatgcg ccgcactggc tggtgtcgca gccgacgtgc
     8701 gtcccggtga gccggaggtg atcgcgcagc gcctcgacca gcagagtggt cgacgcacac
     8761 tcgatgatat gagcttgtcg attgaccttg agcttgatgg tggccatggg tcgtccccct
     8821 tgcgatccca accctcggag atccaggccg gtggcgcaat cgattgtttc atctaaatga
     8881 caggacgcag tgtgccggga gtaccggcgg cgctaggctt gccgggtggc ccccgaccct
     8941 gccgcctggc ggcgcgactt cccggcgctc gacgcgaagg tccatggccg ccgcctggcc
     9001 tacctcgaca gcgcctcgac ctcgctcaag ccgcgggcgg tgatcgacgc ggtggtccag
     9061 gtgttcaccc accaggccgg caatgtccat cgcggcgtcc acgcgctgtc cgaggcggcg
     9121 accgaggcct atgacgccgc gcgcgacgcc atcgcgcggt tcatcggcgg ccgcccggac
     9181 gagatcgtgc tgaccggcgg cacgaccgaa gcgatcaacc tgatcgccca cagctggggg
     9241 cgggcacacg tcgggccagg cgatgcggtg atcgcgaccg cgctggagca tcactcgaac
     9301 ctggtgccgt ggcaagtgct gtgcgccgag cgcggcgcgg tcctgcgaat tgctccgatc
     9361 gacgatcgcg gccggatcga cctggcggcc ctggccggcc tgctcgatga gcgcgtgcgc
     9421 ctggtcgcgg tgtcgcagct gtccaacgtc gtggggacga tcgccccgat cgccgagatc
     9481 gcccggctcg cccacgccgc cggcgcggtg gtcctggtcg atggggcgca ggcggtcgcc
     9541 cacctgccgg tcgacgtcgc ggcgctgggc tgcgacttct atgcgttctc cggccacaag
     9601 ctgttcgcac cgaccggcac cggggtgctg tggggccggc gcgcgctgct cgccgcgatg
     9661 ccggcgtggc agaccggcgg cgagatggtc gccgcggtcg actacgagcg cgcccggctg
     9721 cgcgagccgc cgcaccgctt cgaggccggc accccgaaca tcgcgggcgt gatcggcctg
     9781 ggcgccgcga tccgctacct cgcgggcatc
//
LOCUS       DASVOR010002150         2930 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_81620,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002150 DASVOR010000000
VERSION     DASVOR010002150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2930
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81620"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1515
                     /locus_tag="VF516_39905"
     CDS             <1..1515
                     /locus_tag="VF516_39905"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HEX8113984.1"
                     /translation="DALALWALTCVEPRTGADDDELEVPDALRAAVVAEPAPRDDEAV
                     LARWAWLDQEIPPLVSRPPDRTLTQRIDRVLLHRSVGFAAFIAVMSVLFMSLFWWAQP
                     VIDAIDDLFKGCGGALQGVLGAGIFADFLRQGVIGGVGTVLKFLPQILLLFLFLGFLE
                     DCGYLARVAYLMDRIMRSMNLHGRAFVPMLSGFACAVPALMATRTMERRRDRILTMMV
                     VPLMTCSARLPVYTLVIGALIPGSLWIKSLLMVGMYLFSVVTALIAAWVLSRTVKPLR
                     AKRLPFVIELPPYRWPRFGGVVRMMWSKSSMFLREAGTVILACSIALWALLYFPRHVP
                     DGTPDYDALIERAASDDAKASLRAEKRAILLENSYGGRIGHAIEPAIKPLGFDWKIGV
                     GIIGAFAAREVFVSTLGIVYAVGDEADEESEPLRQALRDARDSHGARAYTPLVGISLM
                     IFFALACQCMSTLAVVRRETASLRWPLFLFGYMTALAWVTSFCVFQLGKLLGLG"
     gene            1627..1977
                     /locus_tag="VF516_39910"
     CDS             1627..1977
                     /locus_tag="VF516_39910"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8113985.1"
                     /translation="MQYLLLIYEEEKVWNTLSEAERGRIYGEYMTFTGEIKNSGHWKG
                     GNPLQPIHTATTVRVRDGKVSTTDGPFAETREQLGGYYLVEAKDLDEATRLAARVPGA
                     RLGSIEVRPVMPIP"
     gene            2054..>2930
                     /locus_tag="VF516_39915"
     CDS             2054..>2930
                     /locus_tag="VF516_39915"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8113986.1"
                     /translation="MQSAAAIEAVFRRERARVLATTIRVTHGDFDLAEEAVQDAFAAA
                     IARWPVEGTPEEPRGWLISVARHKAIDTIRRRVKLREIVSEQAAEATGEASEDAAPAE
                     PVAVADDRLRLIYTCCHPALATEAQIALTLRTLGGLTTEEIARAFLTSAVTMAQRLVR
                     AKSKIRDAGIPYEVPEASQLPERTGAVMAVVYLIFNEGYAATAGDAWIRRDLCREAIR
                     LGRLVAELTGAPEARGLLALMLLHDARRDARTDEHGDVVLLEDQDRARWDQAQIAEAL
                     ALVPDALRGGPGPYSL"
BASE COUNT          386 a         1040 c         1050 g          454 t
ORIGIN      
        1 gacgcgctcg cgctgtgggc gctgacctgc gtcgagccgc ggaccggcgc cgacgatgac
       61 gagctcgagg tgcccgatgc gctgcgcgcc gcggtggtcg ccgagccggc gccgcgcgac
      121 gacgaggccg tgctcgcccg ctgggcgtgg ctcgaccagg agatcccgcc gctggtgtcg
      181 cggccgcccg atcgcacgct gacccagcgc atcgaccggg tgctgctcca ccgctccgtc
      241 gggttcgccg cgttcatcgc ggtgatgtcg gtgctgttca tgtcgctgtt ctggtgggcg
      301 cagccggtga tcgacgcgat cgacgacctg ttcaaggggt gcggcggggc gctccagggc
      361 gtgctgggcg ccggcatctt cgccgatttc ctgcgccagg gcgtgatcgg cggcgtcggc
      421 acggtgctca agttcctgcc ccagatcctg ctcctgttcc tgttcctggg cttcctcgag
      481 gactgcggct acctcgcgcg cgtcgcgtac ctgatggatc gcatcatgcg atcgatgaac
      541 ctgcacggcc gcgcgttcgt gccgatgctg tcgggcttcg cgtgcgcggt gccggccctg
      601 atggcgaccc gcacgatgga gcgccgccgc gaccgcatcc tgacgatgat ggtcgtgccg
      661 ctcatgacct gctcggcgcg cctgccggtc tacaccctcg tgatcggcgc gctgatcccc
      721 ggctcgctct ggatcaagag cctgctcatg gtcggcatgt acctgttcag cgtcgtgacc
      781 gcgctgatcg cggcgtgggt gctgtcccgg acggtcaagc cgctccgggc caagcgcctg
      841 ccgttcgtga tcgagctgcc gccgtatcgc tggccgcggt tcggcggcgt cgtgcgcatg
      901 atgtggagca agtcgtcgat gttcctgcgc gaggcaggca cggtgatcct cgcgtgctcg
      961 atcgcgctgt gggccctgct ctacttcccg cgccacgtgc ccgacgggac ccccgactac
     1021 gacgcgctga tcgagcgcgc cgcaagcgac gacgccaagg cgagcctccg ggccgagaag
     1081 cgggcgatcc tgctcgagaa cagctacggc gggcggatcg gccacgcgat cgagcccgcg
     1141 atcaagccgc tcggcttcga ctggaagatc ggggtcggga tcatcggtgc gttcgccgcg
     1201 cgcgaggtgt tcgtgtcgac gctcggcatc gtctatgcgg tcggcgacga ggccgacgag
     1261 gagtccgagc cgctgcgcca ggcgctgcgc gacgcgcgcg acagccacgg cgcgcgcgcc
     1321 tacacgccgc tggtcgggat ctcgctgatg atcttcttcg cgctcgcctg ccagtgcatg
     1381 agcacgctgg ccgtggtgcg ccgcgagacc gccagcctgc gctggccgct gttcctgttc
     1441 ggctacatga ccgcgctcgc gtgggtcacc agcttctgcg tgttccagct cggcaagctg
     1501 ctcggcctgg gctgattctc ccgtcgcggc ggcgggttat gcgattcgac gcacgctacc
     1561 gcgaggcgtg tcgatccggg tggccgccgt tcgacccacg ggtgaatgtc caaccaggag
     1621 acgcccatgc aatacctgct gctgatctac gaagaggaga aggtctggaa cacgctgtcg
     1681 gaggccgagc gcggtcggat ctacggcgag tacatgacgt tcaccggaga gatcaagaac
     1741 tccggtcact ggaaaggcgg caacccgctg cagccgatcc acaccgcgac caccgtgcgc
     1801 gtgcgcgacg gcaaggtgtc gaccaccgat ggcccgttcg ccgagacccg cgagcagctc
     1861 ggcggctact acctcgtcga ggccaaggat ctcgacgagg cgacccggct cgccgcgcgg
     1921 gtccccggcg ccaggctcgg ctcgatcgag gtccggccgg tgatgccgat cccgtgagct
     1981 cgggatcgct cgcagcccgc gcacgcgcct ggtccgcgag ccggtgcgcg ggccgtgatg
     2041 tatagatccg ccgatgcaga gcgcggcggc gatcgaagcg gtgtttcgcc gcgagcgggc
     2101 gcgcgtgctc gcgaccacga tccgcgtcac ccacggcgac ttcgatctcg ccgaggaggc
     2161 ggtgcaggac gcgttcgccg cggcgatcgc gcgctggccg gtcgagggca cgcccgagga
     2221 gccgcgcggc tggctgatct cggtcgcccg ccacaaggcg atcgacacga tccgccgccg
     2281 ggtcaagttg cgcgagatcg tcagcgagca ggccgccgag gccaccggcg aggcgtccga
     2341 ggacgcggcc ccggccgagc cggtcgcggt cgccgatgac cggctgcgcc tgatctacac
     2401 ctgctgccac cccgcgctcg ccaccgaggc gcagatcgcg ctgaccctgc gcacgctcgg
     2461 cgggctgacc accgaggaga tcgcccgcgc gttcctgacc tcggcggtga cgatggcgca
     2521 gcgcctggtg cgcgccaagt ccaagatccg cgatgccggg atcccctacg aggtccccga
     2581 ggcgtcgcag ctccccgagc gcaccggcgc ggtgatggcg gtggtctacc tgatcttcaa
     2641 cgagggctac gccgcgaccg cgggcgatgc gtggatccgc cgcgacctgt gccgcgaggc
     2701 gatccggctc ggccggctgg tcgccgagct caccggcgcc cccgaggccc gcgggttgct
     2761 cgcgctgatg ctgctccacg acgcgcggcg cgacgcccgc accgacgagc acggcgatgt
     2821 cgtgctgctc gaggaccagg atcgcgcgcg ctgggaccag gcgcagatcg ccgaggcgct
     2881 cgcgctggtc cccgatgcgc tgcgcggcgg gcccgggccg tacagcctgc
//
LOCUS       DASVOR010002151         1616 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_461175,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002151 DASVOR010000000
VERSION     DASVOR010002151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461175"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="VF516_39920"
     CDS             <1..1476
                     /locus_tag="VF516_39920"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8113987.1"
                     /translation="LAIAGLSFEPYLTWLDEDNEAFAGVSPWSSVIRTGAEAAIPTLL
                     ADDQAWVAARAARLATTLAHRPPGGKLAITHARRFDSERRTVVPDATVVIDGDRIVAV
                     GDAATPVPAGARVIDAHGRTLLPGLWDMHVHLFPGDGTLQLASGITTVRDLGNDLEQL
                     AARIARFDAGTELGPRVVRAGLIDEKGPFTAPTGLAVSTLEEATAAVARYADAGYAQI
                     KIYSSIPPPWVPAIAAAGHARGLRVSGHIPTGMNAAQAVEAGYDEIQHVNFLFLRFLA
                     GPEDDTRTPLRFTRVAERGAELDLAGPEVRQFLDLLAAHHTVLDPTLVAFDNMFISDP
                     GELDPVLVPYENRLPAQTVRFGRNGGLPAPDGKRAKFRASYAAMLRMVKAAWDRKIPI
                     VAGTDDIAGLSLPRELELYVEAGIPAAPVLAIATIGAARVMRRDAESGSIAIGKRADL
                     LLIDGDPIRDIAAVRNTVAVVCRGVVYDPAELFAAVGMRPR"
BASE COUNT          212 a          633 c          553 g          218 t
ORIGIN      
        1 ctcgcgatcg ccggcctgag cttcgagccc tacctgacct ggctcgacga ggacaacgag
       61 gcgttcgccg gggtatcgcc gtggtcgtcg gtgatccgca ccggcgcaga ggccgcgatc
      121 ccgacgctgc tcgccgacga tcaggcctgg gtcgccgcgc gcgccgcgcg gctcgcgacc
      181 acgctggcgc atcgccctcc cggcggcaag ctcgccatca cccacgcgcg gcggttcgac
      241 agcgagcgcc gcaccgtcgt gcccgacgcc acggtggtca tcgacggcga tcgcatcgtc
      301 gcggtcggcg atgccgcgac gccggtgccc gccggcgccc gggtgatcga cgcgcacggc
      361 cgcaccctgc tccccggact gtgggacatg cacgtccacc tgttccccgg cgacggcacg
      421 ctgcagctcg cgtcgggcat caccacggtc cgcgacctgg gcaacgacct cgagcagctc
      481 gccgcccgca tcgcgcggtt cgacgccggc accgagctcg ggccgcgcgt ggtgcgcgcc
      541 ggcctgatcg acgagaaggg gccgttcacc gcgccgaccg gcctggccgt gagcacgctc
      601 gaggaggcca ccgccgcggt cgcgcgctac gccgacgccg gctacgccca gatcaagatc
      661 tacagctcga tcccgccgcc gtgggtcccg gcgatcgcgg cggccggcca cgcccgcggc
      721 ctgcgggtca gcggccacat ccctaccggg atgaatgccg cgcaggcggt cgaggccggc
      781 tacgacgaga tccagcacgt caacttcctg ttcctgcgct tcctggccgg gcccgaggac
      841 gacacccgga cgccgctgcg gttcacccgg gtcgccgagc gcggcgccga gctcgatctc
      901 gccggccccg aggtccggca gttcctcgac ctcctggccg cccaccacac cgtgctcgac
      961 cccacgctgg tcgcgttcga caacatgttc atcagcgacc ccggtgagct cgacccggtg
     1021 ctcgttccat atgagaaccg gctcccggcg cagaccgtgc gattcggccg gaacggggga
     1081 ctgccggcgc ccgacggcaa gcgcgcgaag ttccgcgcct cgtacgccgc gatgctgcgc
     1141 atggtcaagg cggcgtggga ccgcaagatc ccgatcgtcg ccggtaccga cgacatcgcc
     1201 gggctgagct tgccccgtga gctcgagctc tacgtcgagg ccgggatccc ggccgccccg
     1261 gtgctggcga tcgccacgat cggcgctgcg cgcgtcatgc ggcgcgacgc cgagtccgga
     1321 tcgatcgcga tcggcaagcg cgcggacctc ctgctgatcg acggcgatcc gatccgcgac
     1381 atcgcggcgg tccgcaacac cgtcgccgtg gtgtgccgcg gcgtggtcta cgacccggcc
     1441 gagctgttcg ccgcggtcgg catgcggccg cggtgacccg gggcccgcgc tgtctatccc
     1501 ggttgacatc cgccgccgcc ggtagacgta acctgtggtg atcacgagcc ggcgatccgg
     1561 cacgccggct gctccagcgc ctggtcgccc tccgggtcga cccactccac gcgctg
//
LOCUS       DASVOR010002152         3255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_257347,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002152 DASVOR010000000
VERSION     DASVOR010002152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3255
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257347"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..358
                     /locus_tag="VF516_39925"
     CDS             95..358
                     /locus_tag="VF516_39925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113988.1"
                     /translation="MPAPTEPPVPARGDGTEDVPGEAPGEAPGEAIDEARRTSFDHKA
                     ELYDAMRPSYPAALIDHLIDRHGGSIELPYVTLAFVAFARAGS"
     gene            366..794
                     /locus_tag="VF516_39930"
     CDS             366..794
                     /locus_tag="VF516_39930"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8113989.1"
                     /translation="MTLSSCAMFKQTHPILGTRDIKRAIAFYTHQLGFRLAFGDQTDP
                     PNYVGFRRDAVELHMQFQFEHEMGTIRLRFLVEDPDALLAEYQQRGVECTPGGIRDTP
                     WGTREFALYDLDRNALTFYRQRVTVRPFGADSRGAARRPA"
     gene            1007..1159
                     /locus_tag="VF516_39935"
     CDS             1007..1159
                     /locus_tag="VF516_39935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113990.1"
                     /translation="MTFAPAAVIGAATAAPAAVLRLPHGIVIEIDGVSPAWVAALACE
                     LARSAS"
     gene            1156..1533
                     /gene="tnpB"
                     /locus_tag="VF516_39940"
     CDS             1156..1533
                     /gene="tnpB"
                     /locus_tag="VF516_39940"
                     /inference="COORDINATES: protein motif:HMM:NF017528.4"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEX8113991.1"
                     /translation="MILLPRAVRVYFATQPTNLRQSFEGLTNQIRHALGYDPLSGHVF
                     VFLNRRRNQVKLILWTRGGFTIVHKRLERGTFTFPARVAEGVASVAIDVHELAVLLEG
                     IEVDELKTKPRWEPTPASRSTLI"
     gene            1608..>3255
                     /locus_tag="VF516_39945"
     CDS             1608..>3255
                     /locus_tag="VF516_39945"
                     /inference="COORDINATES: protein motif:HMM:NF033517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEX8113992.1"
                     /translation="MVIDTSSPDEPEPASAEPELRGSVLDVLRTLLGDHQERDTVLSI
                     VGQLVARNTDLERRVARLAARFKPSEQVSTAQLVLFLEGLKRRPEPPGPEGETTDDHA
                     ALVAADDELRTASGIDEGEDDDVTKLRAPPPPRQPRSPTPPAHLRRVANPIAVPAGQR
                     ACPRCGGERQCIEPHVTEVIELLPPEVIVRRDLREQLTCVACEGEPVRAPAGETVVPN
                     GKLGLGLVASILIEKYLDGLPLHRQRDRYRRLGLDIAVSTLADQVKWCTDLLQPVWRA
                     LLAEIIDARVMHLDGTSLPVQDSEAAGGKRIGALWGYVGVNAGEVLAAYLYVSSGKKA
                     GQRPNERGPEDILALRRGLTVADASNLFDASFARPDLLECGCNMHARRYFVKALDAGD
                     QRAALPLAAYKRLYKIEHEIRDLDDDAKLAARQARSKPVWDELAKWCALRKQHEPPAS
                     KLGVALRYFTNHQVALARFLEYGYLPLDNGIVERLHVRTALTRKNFLFAGSDAGGERA
                     AIAYTILGSCRLAGVDPREYLADVLPRLTGRIRLVDLPALL"
BASE COUNT          494 a         1139 c         1127 g          495 t
ORIGIN      
        1 agatcgagga agggatctga tccgcgcaga ggtcgatcac agcgtcgagg cttctgccgg
       61 cgaccgtcgc gcggccatgc tagcgtgccg cgccatgccc gcgccgaccg agccgcccgt
      121 ccctgcccgc ggcgatggga cggaagatgt gcccggagag gcgcccggag aggcgcccgg
      181 agaggcgatc gacgaggcgc ggcgcacctc gttcgaccac aaggcggagc tctacgatgc
      241 gatgcgacca tcgtaccccg cggcgctgat cgaccacctg atcgaccgtc acggcggcag
      301 catcgagcta ccctatgtca cgctcgcgtt cgtggcgttc gcgcgcgcgg ggtcgtgatc
      361 cgacgatgac gctatcctcc tgtgccatgt tcaaacaaac gcacccgatc ctcggcacgc
      421 gggacatcaa gcgggcgatc gccttctaca cgcatcagct cgggttcagg ctggcgttcg
      481 gagaccagac cgatccgccg aactacgtcg gatttcgccg cgacgcggtc gagctccaca
      541 tgcagtttca gttcgagcac gagatgggta cgattcgact gcggtttctg gtggaggacc
      601 cggacgcgct ccttgccgaa tatcagcagc gcggcgtcga gtgcactccc ggtggcattc
      661 gcgatacgcc gtggggcacg cgcgagttcg cgctctacga cctggatcgc aacgcgctca
      721 cgttctaccg gcagcgcgtg accgtccgac cgtttggcgc cgatagccgc ggcgcggcgc
      781 ggcgccccgc ttgacggcgg gcgaggctcg gggcatggcg aaacgcccct cttcagcagc
      841 tcgacgcagg cattggaccg aagccgacgc acgcggcgtc cttgccgagt gggagcacag
      901 cggcaagaca ctcgaggcat tcgctcggtc gcgcggcctg gtcccgcagc ggcttgcttg
      961 gtggaggaag cgactgcgcg ccgctcggcc cgagacgagc acggcgttga ccttcgcccc
     1021 cgccgcggtg atcggcgccg ccacggccgc ccccgcggcg gtgcttcggc tgccccacgg
     1081 gatcgtgatc gagatcgacg gcgtgtcgcc ggcgtgggtc gccgcgctcg cgtgcgagct
     1141 ggcgaggtcg gcgtcgtgat cctcctacct cgcgcggtgc gggtgtactt cgccacccag
     1201 ccgacgaatc tgcggcagtc gttcgagggg ctcaccaatc agatccgcca cgcactcggc
     1261 tacgacccgc tcagcggcca cgtgttcgtg ttcctcaatc gtcgcaggaa ccaggtcaag
     1321 ttgatcctgt ggacgcgcgg cgggttcacg atcgtgcaca agcgcctcga gcgcgggacg
     1381 ttcacgttcc cggcccgcgt cgccgagggc gtcgcgagcg tcgcgatcga cgttcatgag
     1441 ctggcggtgc tgctcgaagg gatcgaggtc gacgagctca agaccaagcc gcggtgggag
     1501 ccgacgccgg cgtcgcgctc gacgctgatc taacgatcgc tcacatcgat cgcgcgcgcg
     1561 cgtcgcacct cttgcatctg atcgcgtcgc gatcatgatg gcagcgcgtg gtcatcgaca
     1621 cctcctcgcc cgacgagccg gagcccgcga gtgcggagcc ggagctgcgg ggctcagtgc
     1681 tcgacgtgct gcgcacgttg ctcggcgatc accaggagcg ggacacggtg ctgtcgatcg
     1741 tcggccagct ggtcgcgcgc aacaccgacc tcgagcggcg ggtcgcgcgc ctggcggcgc
     1801 ggttcaagcc gagcgagcag gtgtcgacgg cgcagctcgt gctgttcctc gaggggctca
     1861 agcgccgccc cgagccgccg ggacccgagg gcgagacgac cgacgatcac gccgcgctgg
     1921 tggctgccga cgacgagcta cggactgcat ccgggatcga cgagggcgag gacgacgacg
     1981 tcaccaagct gcgtgcgcca ccgccgcccc ggcagccgcg gtcgccgacg ccgcccgcgc
     2041 acctgcggcg ggtcgccaat ccgatcgcgg tgcccgccgg ccagcgcgcc tgtccgaggt
     2101 gcgggggcga gcgccagtgc atcgagccgc acgtcaccga ggtgatcgag ctgctgccgc
     2161 ccgaggtgat cgtgcgccgc gatctccgcg agcagctcac ctgcgtggcc tgcgagggcg
     2221 agccggtgcg cgcgccggcg ggcgagaccg tggtgcccaa cggcaagctc gggctcgggc
     2281 tcgtcgcgtc gatcctgatc gagaagtacc tcgacgggct gccgctgcac cgccagcgcg
     2341 atcgctaccg ccggctcggc ctcgacatcg cggtatccac actcgccgac caggtgaagt
     2401 ggtgcacgga tctgctccag cccgtgtggc gggcattgct cgccgagatc atcgatgccc
     2461 gcgtgatgca cctcgacggc acgagcctgc cggtccagga cagcgaggcg gccggtggca
     2521 agcggatcgg cgcgctgtgg ggctacgtcg gggtgaacgc gggcgaggtc ctggcggcgt
     2581 acctctacgt gtcctcgggg aagaaggccg gacagcggcc caacgagcgc gggcccgagg
     2641 acatcctcgc gctacgcaga gggctcacgg tcgccgacgc atccaatctg ttcgatgcga
     2701 gcttcgcgcg tcctgatctc ctcgagtgcg ggtgcaacat gcacgcgcga cgctacttcg
     2761 tgaaggcgct cgacgccggc gaccagcgcg ctgcgcttcc gctcgccgcc tacaagcgac
     2821 tctacaagat cgagcacgag attcgcgatc tcgacgacga cgccaagctc gccgcgcggc
     2881 aagcgcgcag caagccggtc tgggacgagc tcgcgaagtg gtgcgcgctg cgcaagcagc
     2941 acgagccgcc cgcctccaag ctcggcgtcg cgctccgcta cttcaccaat caccaggtcg
     3001 cgctcgcgcg tttccttgag tatggctatt tgccattgga caacggcatt gtcgagcgac
     3061 tccacgttcg cacggccctg actcgcaaga atttcctgtt cgcgggctcg gacgctggtg
     3121 gcgagcgcgc cgcgatcgct tacacgatcc tcggctcgtg ccgtcttgca ggtgtcgacc
     3181 cgcgcgagta cctcgccgac gtcctgccgc ggctgaccgg gcgcattcgc ctcgtggacc
     3241 tgccggcgct gctcc
//
LOCUS       DASVOR010002153         4631 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_198479,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002153 DASVOR010000000
VERSION     DASVOR010002153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4631
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198479"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="VF516_39950"
     CDS             complement(<1..556)
                     /locus_tag="VF516_39950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113993.1"
                     /translation="MRTLRRKLGYGLLGGAAGLLAMELVKRATAPLVRRRAPRPTGEE
                     LTARTSMSVLGRHHGPEESATDAFGRILYENVYGRSPSPETRRKLSWAVHIGYGLVVA
                     SLYGAVRGGRDHGVARSVISGALFGAGLWLLGDELVLPLLGLSDKPTTYPVTSHLQSL
                     AQHLGFGVATAAATGALLDGHRLRR"
     gene            711..4025
                     /gene="fdh"
                     /locus_tag="VF516_39955"
     CDS             711..4025
                     /gene="fdh"
                     /locus_tag="VF516_39955"
                     /EC_number="1.17.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1293..1295,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HEX8113994.1"
                     /translation="MARIRLPLIDRLRRWPLVRQVAAGDATGLGDTARSPRTDALRPR
                     IDGADRVVGSICPYCGVGCGQLVYLKHGKITDIEGDPDSPISEGCLCPKGAATMQLVT
                     GEHRERRVLYRRPGGTAWEVLALETAMDMVADRVQRTRDRTWQEQLDGGQRVQRTMAI
                     AHIGGATLDNEENYLLKKLYTALGIVQVENQARIUHSSTVPGLGISFGRGGATTFQQD
                     LQNADCILIQGSNMAECHPVGFRWVMKAKERGATIVHVDPRYTRTSAVADVHVPLRAG
                     TDVAFLGGLIRYVIEHERYFRDYVVPYTNAATIISEDFRDTEDLDGLFSGYDPKTGAY
                     DPRSWQYAGIDVVPAAGHKEIVAQLGAGRPGKAMDRVVNEQRDESLSHPRCVFQIVRR
                     HFARYTPEVVEQICGVPRDQLVRIAELLCDNSGRERTTAICYAVGWTQHSIGVQYIRA
                     ASILQQLLGNIGRPGGGILALRGHASIQGSTDIPTLFNLLPGYLPMPHHTHAADLDTY
                     IRSHRSDSGWWSEMPKYIVSLLKAWFGAAATRDNGYLYSRLPHLTGDHSHLATIAMMA
                     DGKLEGYFVMGENPVLGTVGGPLQRKGLRALDWLVVRDFAPTETAEFWRLGPEFERGE
                     VRTEDIRTEVFFFPAAAHTEKDGSFTNTQRLLQWHHAAIEPPGDARSDLWFAYHLGRR
                     LKQRYAHTIGDRDLAIRALTWDYPTHGKVAEPSAEAVLREINGYRVATGEPVSGFTEL
                     ADDGSTACGCWLYSGCYAGGVNQPARRKPRTQQSYVAPEWGWAWPANRRLMYNRASAA
                     PDGTPWSERKRYIWWDAANRRWTGEDVPDFIADRPPDYRPGDDAHGLATLRGDAPFLM
                     QSDGRSWLFAPSGIIDGPLPAHYEPQESVIANPLYRQQCNPARREWRRPDNPYHAAAR
                     DRRFPYVLTTFRLTEHHTAGGMSRWLSWLSELQPAMFCEVSPELAAEVGLRNGGWATI
                     RTARAEIECRVLVTRRIRPLVIGGTVVHQIAIPIHFGGIGRVRGDAANDLFELVIDPN
                     VHIPESKVTTADVVPGRRSRGRTAAVDGPFAIAGRDPGPLALLRRDLPGVGQPGNEPV
                     QIEPVQIEPVQIESKP"
     gene            4022..>4631
                     /locus_tag="VF516_39960"
     CDS             4022..>4631
                     /locus_tag="VF516_39960"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEX8113995.1"
                     /translation="MNRTVGFFTDTTLCIGCKACEVACTEWNQLPDDGFHLTAMSYDN
                     TAALGASTWRHVTFAERPDGPGGIAWRMMSDVCKHCARAGCLEACPTGAILRTEHGSV
                     YVQPDVCNGCGYCVPACPFGVIARRDDDGRAFKCTLCYDRLNGGLEPACAKACPTDSI
                     QFGPLDELRVRAADRQAALRARGLPAQLYGADPADQPGTGGLH"
BASE COUNT          691 a         1622 c         1703 g          615 t
ORIGIN      
        1 agcggcggag gcgatggccg tcgagcagcg cgccggtggc cgcggcggtc gcgacgccga
       61 acccgaggtg ctgggcgagc gactgcaggt ggctggtcac cggataggtc gtcggcttgt
      121 ccgacaggcc gagcagcggg agcacgagct cgtcgccgag cagccacagg ccggcgccga
      181 acagcgcgcc gctgatcacc gagcgggcga cgccatgatc gcgcccgccg cggaccgcgc
      241 cgtagagcga tgccaccacc aggccgtagc cgatgtggac cgcccacgac agcttgcgcc
      301 gggtctcggg cgacggcgag cggccgtaga cgttctcgta gaggatccgg ccgaacgcgt
      361 cggtcgcgga ctcctcgggg ccatgatgcc ggccgagcac cgacatcgag gtgcgtgcgg
      421 tgagttcctc gccagtgggc ctcggcgcgc gacgcctcac cagcggcgcg gtggcgcgct
      481 tgacgagctc catcgccagg agacccgccg cgccgccgag caggccgtaa ccaagcttcc
      541 ttcgcagggt tcgcatagcg cccagcgatg cagcctgcat tccgcgcagg tgcgccggca
      601 ggagttcccc gcgcgcacag gagacaccgc gccggatcgc gcgtgtgcgg ggcccggcga
      661 gcccggcgca ggatgtgcga tccggtgcgc ggcatgcatc gcgtctcgtc atggctcgca
      721 tccgccttcc gctgatcgat cggctccggc gctggcccct ggtccggcag gtcgccgccg
      781 gcgacgcgac cggcctcggt gacaccgcgc gctcgccgcg caccgacgcg ctgcggccgc
      841 ggatcgacgg cgccgatcgc gtggtgggct cgatctgtcc gtattgcggc gtgggctgcg
      901 gtcagctggt ctatctgaag cacggcaaga tcaccgatat cgagggcgat ccggactcgc
      961 cgatctcgga gggctgcctg tgcccgaagg gcgccgcgac gatgcagctg gtgaccggcg
     1021 agcaccgcga gcgccgggtg ctgtaccgcc ggccgggcgg gacggcgtgg gaggtgctgg
     1081 cgctcgagac cgcgatggac atggtcgccg accgggtgca gcgcacgcgc gaccggacct
     1141 ggcaggagca gctcgacggc ggacagcggg tccagcgcac gatggcgatc gcccacatcg
     1201 gcggcgcgac gctcgacaac gaagagaact acctgctcaa gaagctgtac accgcgctcg
     1261 ggatcgtcca ggtcgagaac caggcgcgga tatgacatag ctcgacggtc cccggtctgg
     1321 ggatctcgtt cggacgcggc ggtgcgacga cgttccagca ggacctgcag aacgccgact
     1381 gcatcctgat ccagggctcg aacatggccg agtgccaccc ggtcgggttc cgctgggtga
     1441 tgaaggccaa ggagcgcggc gcgacgatcg tgcacgtcga tccgcgctac acgcggacca
     1501 gcgcggtcgc cgacgtgcac gtgccgctgc gcgcggggac cgacgtcgcg ttcctcggcg
     1561 ggctgatccg gtacgtgatc gagcacgagc gctatttccg cgactacgtg gtgccgtaca
     1621 ccaacgcggc gacgatcatc tcggaggact tccgcgacac cgaggacctc gacgggctgt
     1681 tcagcggcta cgacccgaag acgggcgcct acgatcccag gagctggcag tacgccggga
     1741 tcgacgtggt gcccgccgcg ggacacaagg agatcgtggc gcagctcggc gccgggcggc
     1801 ccggcaaggc gatggaccgg gtggtcaacg agcagcgcga cgagagcctc tcccacccgc
     1861 gctgcgtgtt ccagatcgtg cgccgccact tcgcgcgcta cacgccggag gtcgtcgagc
     1921 agatctgcgg cgtgccgcgc gaccagctgg tccggatcgc cgagctgttg tgcgacaact
     1981 cggggcgcga gcggaccacc gcgatctgct acgcggtggg ctggacccag cactcgatcg
     2041 gcgtgcagta catccgcgcg gcgtcgatcc tgcagcagct gctcggcaac atcggccggc
     2101 cgggcggcgg gatcctggcg ctgcgcggcc acgcgtcgat ccagggctcg accgacatcc
     2161 cgacgctgtt caacctgctg ccggggtacc tgccgatgcc gcaccacacc cacgcggcgg
     2221 acctcgacac gtacatccgc tcgcaccgct ccgacagcgg ctggtggagc gagatgccca
     2281 agtacatcgt gtcgctgctc aaggcgtggt tcggcgccgc ggcgacccgc gacaatggct
     2341 atctgtactc ccggctgccg cacctcaccg gcgaccactc gcacctggcg acgatcgcga
     2401 tgatggccga cggcaagctc gagggctact tcgtgatggg cgagaacccg gtgctcggga
     2461 cggtcggcgg gccgctgcag cgcaaggggc tgcgcgcgct cgactggctg gtggtgcgcg
     2521 acttcgcgcc gaccgagacc gccgagttct ggcggctcgg gccggaattc gagcgcggcg
     2581 aggtccggac cgaggacatc cggaccgagg tgttcttctt cccggcggcg gcgcacaccg
     2641 agaaggacgg ctcgttcacc aacacgcagc ggctgctgca atggcaccac gcggcgatcg
     2701 agccgccggg cgacgcgcgc agcgatctgt ggttcgcgta ccacctcggc cgccggctca
     2761 agcagcgcta tgcgcacacg atcggcgacc gcgacctcgc gatccgcgcg ctgacctggg
     2821 actacccgac gcatggtaag gttgcagagc cgagcgccga ggcggtgctg cgcgagatca
     2881 acgggtaccg ggtggcgacc ggcgagccgg tgagcgggtt caccgagctc gccgacgacg
     2941 gctcgacggc gtgcggctgc tggctgtact ccgggtgcta cgccggcgga gtcaaccagc
     3001 ccgcccgccg caagccgcgc acccagcaga gctacgtggc gccggagtgg ggctgggcgt
     3061 ggccggcgaa ccggcggctg atgtacaacc gcgcgtcggc ggcgcccgac ggcacgccgt
     3121 ggagcgagcg caagcgctac atctggtggg acgcggcgaa ccggcggtgg accggcgagg
     3181 acgtgccgga cttcatcgca gaccgccccc cggactatcg ccccggcgac gatgctcacg
     3241 ggctggcgac cctgcgcggc gacgcgccgt tcctcatgca gtccgacggg cgcagctggc
     3301 tgttcgcgcc gagcgggatc atcgacggcc cgctgccggc gcactacgag ccccaggagt
     3361 cggtgatcgc caatccgctg taccgccagc aatgcaatcc ggcgcgccgc gagtggcggc
     3421 ggcccgacaa tccgtatcac gccgcggcgc gcgaccggcg gttcccgtac gtgctgacca
     3481 cgttccggct gaccgagcac cacaccgcgg gcggaatgag ccgctggctg tcgtggctgt
     3541 ccgagctcca gccggcgatg ttctgcgagg tgtcgcccga gctcgcggcc gaggtcggcc
     3601 tgcgcaacgg cggctgggcg acgatccgca ccgcgcgcgc cgagatcgag tgccgcgtgc
     3661 tggtcacccg ccggatccgg ccgctggtga tcggcgggac ggtcgttcac cagatcgcga
     3721 tcccgatcca cttcggcggc atcgggcggg tgcgcggcga cgcggccaac gacctgttcg
     3781 agctggtcat cgatcccaac gtgcatatcc ccgagagcaa ggtcacgacc gcggacgtcg
     3841 tcccggggcg gcggtcgcgc gggcggaccg cggcggtcga cggtccgttc gcgatcgccg
     3901 gccgcgatcc ggggccgctg gcgctgctgc ggcgcgacct gcccggcgtc ggtcagccgg
     3961 gcaacgaacc ggtgcagatc gaaccggtgc agatcgagcc ggtgcagatc gagagcaagc
     4021 catgaaccgg acggtcggat tcttcaccga caccacgctg tgcatcggct gcaaggcgtg
     4081 cgaggtcgcc tgcacggagt ggaaccagct gccggacgac gggtttcacc tgacggcgat
     4141 gtcgtacgac aacaccgcgg cgctcggcgc ctcgacctgg cggcacgtca cgttcgccga
     4201 gcgccccgac gggccgggtg ggatcgcgtg gcggatgatg tccgatgtgt gcaagcactg
     4261 tgcgcgggcc ggttgcctcg aggcgtgccc gaccggcgcg atcctccgca ccgagcacgg
     4321 ctcggtctac gtccagcccg atgtctgcaa cggctgcggg tactgcgtgc cggcgtgccc
     4381 gttcggcgtg atcgcccggc gcgacgacga cggtcgcgcg ttcaagtgca cgctgtgcta
     4441 cgaccggctg aacggcggcc tggagccggc gtgcgccaag gcgtgcccga ccgactcgat
     4501 ccagttcggc ccgctcgacg agctgcgggt gcgcgccgcc gatcgccagg cggcgctgcg
     4561 cgcacggggg ctgccggcgc agctgtacgg cgccgacccg gccgaccagc cgggcaccgg
     4621 cgggctgcac g
//
LOCUS       DASVOR010002154         2876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_285079,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002154 DASVOR010000000
VERSION     DASVOR010002154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285079"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VF516_39965"
     CDS             <1..909
                     /locus_tag="VF516_39965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113996.1"
                     /translation="TPSEPMVSRVPTPREPMVSRVPTPREPTVSRVPTAPRVSRVVSE
                     QFTERTTTVRPNALTASEVKALIAQRCALIDRGVDHFTLLGLPVGAPGEAVRTAYLEL
                     ARYLRPDKLVLLGIPAEALDAQRLFAQAGIAFTTLTHPARRAEYMATLHGAVPILPLN
                     APPDRKALAAEAYQRGLQALRADQPVKAVVELAQAVELAPHDVDYGAALGWARFCASS
                     DKAAVAPETRRLLERAIHRSARPEVARLHLGRVERMLGRDPLALQHFREVLELVPGHA
                     EAASEIRAIEARLVLRTSTPRPPRPR"
     gene            complement(920..2143)
                     /locus_tag="VF516_39970"
     CDS             complement(920..2143)
                     /locus_tag="VF516_39970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113997.1"
                     /translation="MNEGQRLQYAIQAAMRAVQARGGEAGSYEWGPGEDQIEQVHNRL
                     KDAIRDRVAQRVHEKLAEMARERVREIVRDRLSSELRAGLLDAQSERGFDFSRIERKL
                     GERLSEALAERMSEAVRSRIGDAVRERLSDVVREAVSDTPWADDAERIASRVREYIEP
                     EIVDRLGEAVRERVREVARERIQQAARERLSEAFQTAIEQRGGAKDPERIMTMVRERL
                     ADAIEQRVAEGMMERLREVVRNCFEDAARERLREALRAAVGQRMQGMQGGGDGAMRMA
                     ATGPWNLAGDGLTMVESLREQIHDVIRDRFSEAVRERVGSAIRLQMDDVLRERLGTVI
                     RQALMEQKTLGGFDPDQFADSVRGRLGHAIRERLTDAIRERLHEVHRDRLRDAIRSAV
                     SQRPVEFTGFGTGTH"
     gene            2558..>2876
                     /locus_tag="VF516_39975"
     CDS             2558..>2876
                     /locus_tag="VF516_39975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8113998.1"
                     /translation="MQDSAAPVRAPDFDALVATDGAHDTSPAHAADARLGVYLHMLAD
                     RISHHVCADRSVIAGPSPSGFSVDLTSAECAQGIHLLRHAWETGVGYARLFPQDRTTL
                     AMLQ"
BASE COUNT          345 a         1150 c          971 g          410 t
ORIGIN      
        1 accccgagcg agccgatggt gtcgcgggtg ccgacgccgc gcgagccgat ggtgtcgcgg
       61 gtgccgacgc cgcgcgagcc gacggtgtcg cgggtgccga ccgcgccgcg ggtgtcgcgc
      121 gtggtgtccg agcagttcac cgagcggacc acgaccgtcc ggcccaacgc gctgaccgcg
      181 agcgaggtca aggcgctgat cgcccagcgc tgcgcgctga tcgaccgcgg cgtcgaccac
      241 ttcaccttgc tcgggctgcc ggtcggcgca ccgggcgagg cggtgcgcac tgcctacctc
      301 gagctcgcgc gctacctgcg cccggacaag ctcgtgctgc tcggcatccc cgccgaggcg
      361 ctcgacgccc agcgcctgtt cgcccaggcc ggcatcgcgt tcaccacgct cacccatccg
      421 gcgcgccgcg ccgagtacat ggcgaccctg cacggcgccg tcccgatcct gccgctgaac
      481 gccccgccgg atcgcaaggc gctcgccgcc gaggcctacc agcgcggcct ccaggcgctg
      541 cgcgccgatc agccggtcaa ggcggtcgtc gagctcgccc aggcggtcga gctcgcgccg
      601 cacgacgtcg attacggcgc cgcgctcggc tgggcccggt tctgcgcgtc gtcggacaag
      661 gccgccgtcg cccccgagac ccgccggctg ctggagcgcg cgatccaccg ctccgcccgg
      721 ccggaggtcg cgcggctcca cctcggccgg gtcgagcgca tgctcggccg cgacccgctc
      781 gcgctgcagc acttccgcga ggtcctcgag ctcgtgcccg gtcacgccga ggcggcgagc
      841 gagatccggg cgatcgaggc ccggctggtc ctccggacct cgaccccccg gcccccgcgc
      901 ccgcggtagc cgcgcgcggt cagtgcgtgc cggtgccgaa cccggtgaac tcgaccgggc
      961 gctgggacac cgcggagcgg atcgcgtcgc gcagccgatc gcgatggacc tcgtggaggc
     1021 gctcgcggat cgcgtcggtc agccgctcgc ggatcgcgtg gccgagccgg ccgcgcaccg
     1081 agtccgcgaa ctgatcgggg tcgaagccgc ccagcgtctt ctgctccatc agcgcctggc
     1141 ggatcaccgt gccgagccgc tcgcgcagga cgtcgtccat ctgcaaccgg atcgccgagc
     1201 cgacccgctc gcgcaccgcc tcgctgaacc gatcgcggat gacgtcgtgg atctgctcgc
     1261 ggagcgactc caccatcgtc aggccgtcgc cggcgaggtt ccacgggccg gtcgcggcca
     1321 tccgcatcgc gccgtccccg ccgccctgca tgccctgcat gcgctggccg accgcggcgc
     1381 gcagcgcctc gcgcaggcgc tcgcgggcgg cgtcctcgaa gcagttgcgc acgacctcgc
     1441 gcagccgctc catcatgccc tcggcgacgc gttgctcgat cgcatcggcc agccgctcgc
     1501 gcaccatggt catgatgcgc tcgggatcct tcgcgccgcc gcgctgctcg atcgcggtct
     1561 ggaacgcctc gctcagccgc tcgcgcgcgg cctgctggat ccgctcgcgc gccacctcgc
     1621 gcacccgctc gcgcaccgcc tcgccgagcc ggtcgacgat ctcgggctcg atgtactcgc
     1681 ggacccgcga cgcgatccgc tcggcgtcgt cggcccacgg cgtgtcgctg accgcctcgc
     1741 ggaccacgtc gctgagccgc tcgcggaccg cgtcgccgat ccggctgcgg accgcctccg
     1801 acatgcgctc ggcgagcgcc tcggacagcc gctcaccgag cttgcgctcg atccgcgaga
     1861 agtcgaaccc gcgctcgctc tgcgcgtcga gcaggcccgc gcgcagctcg ctggacagcc
     1921 ggtcgcgcac gatctcgcgc acgcgctccc gcgccatctc ggcgagcttt tcgtgcacgc
     1981 gctgcgcgac gcgatcgcgg atcgcatcct tgagccggtt gtgcacctgc tcgatctggt
     2041 cctcgcccgg accccactcg tagctgccgg cttcgccgcc ccgggcctgg acggcgcgca
     2101 tcgcggcctg aatcgcatac tgcagtcgct gcccttcatt catctctggc atcatcttgg
     2161 tggtctccat cattctcgga gcagttggct tcgagtacgg cgcggtcaag caaggcgtgg
     2221 accgcgagcc cagcgcgctg gacgtgggtt ggccagggca acggcgcact cgtgttccag
     2281 aattgccgtg cgaaatggtc gcgaacgcga tcaccaacgc tgcgctcgat gatgtgagat
     2341 ccggagtctc ggcgcggagt ctttctcgtc ggcgcgacgc gatcacggcg ctcatccggc
     2401 gcgtgctccg gaggatgccg cctgccgcgc ggcgcaggtc cggcgggtcc acgcgaccgg
     2461 cgccacctgc ttcgcgtccg ggactccgat ccaggcctcg atccgcctgt tcggcccgct
     2521 cgacctgccg ttcgcgaccc aggccggccc gcagctggtg caggacagcg ccgcgccggt
     2581 gcgcgctccc gacttcgacg cgctggtcgc caccgacggc gcccacgaca ccagccccgc
     2641 ccacgccgcc gacgcccggc tcggcgtcta cctccacatg ctcgccgacc ggatctcgca
     2701 ccacgtgtgc gccgaccgct cggtgatcgc gggcccgtcg ccctcggggt tctcggtcga
     2761 cctgaccagc gccgagtgcg cccagggcat ccacctcttg cgccacgcct gggagaccgg
     2821 cgtcggctac gcgcggctat tcccccagga ccgcacgacg ctggcgatgc tgcagg
//
LOCUS       DASVOR010002155         3708 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_315598,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002155 DASVOR010000000
VERSION     DASVOR010002155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315598"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            677..997
                     /locus_tag="VF516_39980"
     CDS             677..997
                     /locus_tag="VF516_39980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8113999.1"
                     /translation="MFDTLEVSLTVLEQLAPVETKIRQRRRSLADEIGRAAEPVALNV
                     SEARQCAGLDRADLFRRAAGSASELTTALRIARARGYITATEHAAVDAALDRVRAMLW
                     RLTH"
     gene            complement(1435..2205)
                     /locus_tag="VF516_39985"
     CDS             complement(1435..2205)
                     /locus_tag="VF516_39985"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8114000.1"
                     /translation="MKVELRSVTRTKRRSEQEQLVLFKPRGGKRRGAGRPPKGKRAGS
                     PHKERPFLHARYPVHVVLRVVGAVGNLRRRCVYQAVREATLVTALREDFRIVQLSIQR
                     THVHLLVEANDKHALARGMQGFQISAAKHLNAAISKGKPGPRRRGVVFPDRYHAEIIR
                     SPRQARHALSYVMNNWRKHQEDLRAPLSGWTIDWFSSAAMFTGWAEYGNEPFLWRGPA
                     TYDPLVVYQPRTWLLREGWKRSGSISCREVPSAKQQPR"
     gene            complement(2648..>3708)
                     /locus_tag="VF516_39990"
     CDS             complement(2648..>3708)
                     /locus_tag="VF516_39990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114001.1"
                     /translation="WYAVAQGALCLSAAWAILALYLRGTPVRPGEPAPTPARDAGLLW
                     LGLAVALWGITGGLLLLPLPESLVQALRTVLSSANNACLLISASHLDYGPALLQRASD
                     YPRWNQLALAGSLAVALVTIALYAALGPAASVARLPDFALSSITLLLYGFGLFRSFRR
                     RGFAPLAVLAAVAVGLQFAAQVPEVLDVAQLGVSDEPRWTLNLVSKAMVLVAFLSLAM
                     SWVHEVAQRPSHSAIRLRFTGRRAGARYVVELGGRTLEMRETPHRDLLSLAVARVGGG
                     GADGGGWVSLLDLVGRLDDSRIRRMREDLRAAGLDKEIEANGHKSYRLAIEPQHIDFD
                     RDALARVPDLEAIAKQIA"
BASE COUNT          579 a         1321 c         1293 g          515 t
ORIGIN      
        1 gcacggggcg accgcggccg cgtcgggggg cgccgcatcc agcgcggcat cggcccgcgc
       61 ggcgtcgcgc acggcagcgt cggcggccgg gtgcaggctg tccccgcagg ccgcggcgaa
      121 gcaccacggg ggcgtcgcac acagcgccgc ccggaccgcc aggatcgacc accgcatcag
      181 agcgagtctg cgaccgccgc tcgcgcggcg ccaggggcaa tgccgtcact gacaccactg
      241 ctgctgcagc agccgcttcg gcttcggctt ctgctgccgc ttcggcttcc gctgccgctt
      301 cggcgaccgc tgccgcttcg gcgaccgctg ccgcttcggc tgcccctgcc gcttcggcga
      361 ccgctgccgc ttcgtcggta agcgtacact gcaagcgcat ggcctcgtgt gcgagtgctt
      421 ccacgctgcg ccgctcgacg cgctaacaca gcggcctccg tgatcgcccg gcaccggggc
      481 gtcgcccttt cgccgctctc gcgcaccatc ccttgccgag cgccggaccg accgacgtga
      541 cgcgaacggc gcccgcacag ctggatccat cgacgaaccg cgggtcgcgg tgcttgcagt
      601 cgtcacggga catgcgctcc gcggcgcgtc gggcgacgac gccccggtgc gaggggcgaa
      661 tcaaagggag gttccgatgt tcgatacgct cgaggtgtcg ctcaccgtgc tggagcaact
      721 cgctcctgta gagaccaaga tccgccagcg ccgcaggtcg ctcgccgacg agatcggccg
      781 cgcagccgag ccggtcgcgc tcaacgtcag cgaagcgcgg cagtgcgcag gcctcgatcg
      841 cgccgacctg ttccgccgcg ccgccggctc ggcaagcgag ctcaccacgg cgctgcgcat
      901 cgcgcgagcg cgagggtaca tcactgccac ggagcacgcg gcggtcgacg ccgcgctaga
      961 tcgggttcgt gcgatgctgt ggcggctcac ccactgagca ccgcctcggc tttgccggcg
     1021 ggagatcccg cgggtcgaca agcaacacga cggcttccac gccgcggttc cacgccagca
     1081 gatcggagcc cgcgttgtct acccccgcag gttacgaggt gcgaaccgcc tcgcaagcgc
     1141 actttccgcg acgatgatca accagcttgg ccgagacacc acgcagagcg agcggatccg
     1201 gactaccacc cgcatcacct gttctggtcg acgatggcag tgcgggctgc gaaggaggac
     1261 gagtccctcg cggacctgcc gcgcaagatc agccatcaga atggagctct cgaacatcgc
     1321 atcggctgcg catcggttcg cctggccctt gcccccgcgc gccgggcatc atcgacaccg
     1381 gcgagctggc gcaccagacc gcgcctcgtc cacatccgca accctcgtgc tcgactaccg
     1441 cggctgttgc ttcgccgacg gcacctcgcg gcaggagatg gagccggatc tcttccagcc
     1501 ctcgcgaagc agccaggtcc ggggctggta cacgaccagc ggatcgtagg ttgccgggcc
     1561 gcgccacagg aacggctcgt tgccgtactc cgcccagccg gtgaacatcg ccgcgctgga
     1621 gaaccagtcg atcgtccagc ccgacaacgg cgcgcggagg tcttcctggt gctttctcca
     1681 gttgttcatc acgtagctca gcgcatgccg cgcctggcgc ggtgaccgga tgatctccgc
     1741 gtggtagcgg tcggggaata ccacgccccg ccggcgtggg cccggcttgc ccttgctgat
     1801 cgccgcgttg agatgcttgg cagccgagat ctggaagccc tgcatgcccc tggccagcgc
     1861 gtgcttgtcg ttggcctcca ccagcaggtg gacgtgcgtg cgctggatgc tgagctggac
     1921 gatgcgaaag tcctcgcgca gtgccgtgac gagcgtggcc tcgcggaccg cctggtacac
     1981 gcagcggcgg cgcaggttgc cgacggcgcc gaccacccgc aggacgacat ggacagggta
     2041 gcgagcatgg aggaacggcc gctccttgtg cggcgagccc gcccgcttgc ccttcggcgg
     2101 gcgaccggct ccacgccgct tgccaccgcg cggcttgaac agcaccagct gttcctgctc
     2161 ggagcgccgc ttcgttcggg tgacgcttcg cagctcgacc ttcacagccc gtagatagat
     2221 gtggagcact gttcagtcaa ttgaaatcgc cgtattttct tgtagcgaga tgtcatcagc
     2281 tggagacatc atcgtaggaa ccgggggtgc agccttgata gatccttgac accttgatcc
     2341 gaggacgtcc ctgacacggg gcgctgggag tccaaggacg tccccgacag accaaccggg
     2401 ttgcggatgt tgaccgcacc tgcggacgag taggtcttgg tcgaaacgtc ggcggcacct
     2461 ggacgcagtt cgccgtcagc ttcggtggcc tgcacctcgg aaccttcgac agcaagcacc
     2521 tcaacggcgg tctccgcatt ccgcgtcgtc gacgcaggaa gccaggcgaa ggatctacga
     2581 ccgcccttgg ggagaggcac agcgcgtcag cggcgacgcg gccgatcgcc ggtgaccggc
     2641 attggcgcta cgcgatctgc ttcgcgatcg cctcgaggtc ggggacgcgg gcgagcgcat
     2701 cgcgatcgaa gtcgatgtgc tgcggctcga tcgccaggcg gtacgacttg tggccgttcg
     2761 cctcgatctc cttgtcgagc ccggcggcgc ggaggtcctc gcgcatccgc cggatccggc
     2821 tgtcatcgag gcggcccacg agatcgagca gcgacaccca gccgccgccg tccgcgccgc
     2881 cgccgccgac ccgcgcgacg gcgagcgaca gcagatcgcg gtgcggggtc tcgcgcatct
     2941 ccagcgtccg cccgccgagc tcgacgacgt agcgcgcgcc ggcgcgccgg ccggtgaacc
     3001 gcaggcggat cgcgctgtgc gacgggcgct gcgcgacctc gtggacccag ctcatcgcga
     3061 gcgacaggaa cgcgaccagc accatcgcct tggacaccag gttgagcgtc cagcgcggct
     3121 cgtccgacac gccgagttgc gccacgtcga ggacctcggg gacctgcgcc gcgaactgca
     3181 ggccgaccgc gaccgcggcg aggacggcga gcggggcgaa gccgcggcgg cggaacgagc
     3241 ggaacaggcc gaacccgtag agcaggagcg tgatcgacga cagcgcgaag tcggggagcc
     3301 gggcgaccga cgcggcgggc cccagggccg cgtagagcgc gatcgtgacc agcgcgacgg
     3361 cgagcgagcc ggcgagcgcg agctggttcc agcgcgggta gtcgctggcg cgctggagca
     3421 gcgccgggcc gtagtcgagg tgcgacgccg agatcaaaag gcaggcgttg ttggcgctgg
     3481 acagcacggt gcgcagcgcc tgcacgaggc tctcgggcag cggaagcagc aacaggccgc
     3541 ccgtgatgcc ccacagcgcg acggcgaggc cgagccagag caggccggcg tcgcgcgccg
     3601 gggtcggggc cggctcgccc gggcgcaccg gcgtgccccg caggtacagc gccaggatcg
     3661 cccaggcagc ggacaggcag agcgccccct gcgcgaccgc gtaccagg
//
LOCUS       DASVOR010002156         1902 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_373882,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002156 DASVOR010000000
VERSION     DASVOR010002156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1902
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373882"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..846)
                     /locus_tag="VF516_39995"
     CDS             complement(<1..846)
                     /locus_tag="VF516_39995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114002.1"
                     /translation="MVDHTETCALGCFTDGSRCFAVTPSNGLGAALDQSAQQGAITLP
                     AGAVVDSDTGAVTSAQQAPIVVTTLTVAQTGGATLRVLLARSWTISDVRVRGSLPVAF
                     VSSDDINIQGAIDVSADGNTSGAGALTCGAGTGGGGAPSDAHFERPPAGSSTGYPAFI
                     WVTNGFGGGGFGSSGGAGGAEYTLSPPGPAGQANGNAELVPLRGGCEGGGDMAQNRGA
                     GGGAVQLVSGRTVHLLTGGVIHVGGGRGAAGALGRISTSDTTPVFGPSGGGSGGGILI
                     EAPSVV"
BASE COUNT          312 a          697 c          616 g          277 t
ORIGIN      
        1 caccacgctc ggcgcctcga tcaggatgcc gccgcccgag ccaccgccgc tcggaccgaa
       61 caccggggtc gtatcgctgg tgctgatccg acccagagcg ccggcggcgc cgcggccacc
      121 gccgacgtgg atcacgccgc cggtgagcag atgaacggta cggccgctga ccagctggac
      181 cgctccgccg ccggcgccgc ggttctgcgc catgtcaccg ccgccttcgc agccaccgcg
      241 cagcggtacc agctcggcgt tgccgttcgc ctgaccggcc gggcccggcg gcgacagcgt
      301 gtactccgca ccgcccgccc cgcccgacga gccgaacccg ccccctccga atccgttcgt
      361 cacccagatg aacgccggat atcccgtcga gctcccggcc ggcgggcgct cgaagtgggc
      421 atcgctgggc gcgccaccgc ccccggtgcc agcgccgcag gtcagcgcgc cggcgccgct
      481 ggtgttgccg tcggcagaca cgtcgatcgc accctggatg ttgatgtcat cgctcgacac
      541 gaacgccacc ggcaagctgc cccggacgcg gacgtcgctg atggtccacg accgggcaag
      601 caggacgcgc agcgtcgcac cgccggtctg cgccaccgtc agcgtggtca ccacgatcgg
      661 cgcctgctgc gccgaggtca ccgcccccgt atcgctgtcg accactgctc ctgccggcaa
      721 cgtgatcgca ccctgctgcg cggattgatc cagcgcagcg ccgaggccgt tcgacggcgt
      781 gaccgcaaag cagcgactgc catcggtgaa acagccgagc gcgcaggtct ccgtatgatc
      841 gaccattccc gatgcgttac aaatctggag catacccccg gtgcacgtgt tctgcgtgca
      901 cgcctgggcg cccgcgtcgc tgcccggcgc agcgtcggag gacacccctc cgtcggcacc
      961 gtgcgagccc gacgagacgc tcccgcacgc cgtcacggcg accgtcatgg cgatcgatga
     1021 gacagacaac ttccacatct gtaccttgcc ttgcgaagtc cgttacgcgc acgacgaatg
     1081 cacgcgatgg gccgatgtcg tctggcgctc aaccgcacgg aactgcacct gcccagcgcc
     1141 aggatgccgg cgagcgaggc cgctcgtgtc tcctctgtca caggccacgg ccggggacag
     1201 cggagacacg gagacacccc cgaccgaaag atcacccgct ccataaccgg gccggtgacc
     1261 gctccggctc cacgtaagca gagctgcgac cacgccgacg cgagcttcac gcccggttca
     1321 cgccgtggcc gacgccgagc gtccgggcac atcgggtcct ccccgcgtcc actggtgaac
     1381 ccgctgcggt ccagggcgga cctgccggcg cgatcaggcg atggccgtcg acgcgcggcg
     1441 agccaggcgc gacacccggt gaaatcaatg cgttacggcg ccaccgatcg gcgcgttgtg
     1501 tagtcgaacg tctgccacgt gccacgacgg cccgacacgg cccgatgtcg ctcgcggcgc
     1561 agcgagacgc ggcgttcgcg atcgcgcagc tcctgcgcca tggatcacga gttgcagtca
     1621 gcgccgagcg tcagattctg gaaggaggaa tacagaggcc actcgcgctg atgtaccggg
     1681 cgcggcttcc gccgtgatat cgaaggatgt caatctagaa agacggtaac gaatatcgtg
     1741 attcagtacg ctcgaccatg tgccggcatg tattgctccg gcgtgatcgt cgcgatctat
     1801 tgatgtcgat gttgctgtag ctgcgtggag ctttccggcg cggcggggca gtcttgcccg
     1861 caggcagcgc tgcgatgggt cgcgacggga aggatagctc ca
//
LOCUS       DASVOR010002157         2050 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_227808,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002157 DASVOR010000000
VERSION     DASVOR010002157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2050
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_227808"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VF516_40000"
     CDS             complement(<1..199)
                     /locus_tag="VF516_40000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114003.1"
                     /translation="MRHAPEVVYSAAMTTGEAHRAIDAVWRIESARLIAGLARMVRDV
                     GLAEELAQDALVAALERWPADG"
     gene            299..1051
                     /locus_tag="VF516_40005"
     CDS             299..1051
                     /locus_tag="VF516_40005"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8114004.1"
                     /translation="MKKNDANKANLVPRILPRGRHKLDPQLVAASQRQRLLEAIVELV
                     AEKGYPEVTIGDIVSRAGTAKRTFYDHFADKLECFLAALEGITDTLVAASARLFAVSG
                     TVRERCEYSMRGYLELLVSMPNTAKVFYLEAVAAGPESVTRRHAVHLKFARNIVGLSR
                     AAAAAGEGQELTELHALAVVGAMHQMIYGQLLDHGPDSLLEIIDDVVEIAVAFLTVRM
                     PGAPTRRPPARRAQKRDTPAARPDATRRDGSR"
     gene            1296..1874
                     /locus_tag="VF516_40010"
     CDS             1296..1874
                     /locus_tag="VF516_40010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114005.1"
                     /translation="MKRPSRGYIVFLLAGVLGGVGGTAWAEDPAPSGGAAPAAPMAMP
                     RKFTGDQQAFFIAGGVADSTTWTIGTTLPAGVTISVGLNINYNGNGLPGPTGMPSSDK
                     FAFSGVLYGAYYIVNKFPVGIAVEAALVEPLSPTAGSPATVVQPGIGIFYAPFPAPIV
                     LGSGLDLQIVIPKNGSTVVQTVTPGLRIVYVF"
BASE COUNT          298 a          718 c          728 g          306 t
ORIGIN      
        1 cgccgtcggc cggccagcgc tccagcgcgg cgaccagcgc atcctgggcg agctcctcgg
       61 cgagcccgac gtcgcgcacc atgcgcgcga ggccggcgat cagccgcgcg gactcgatcc
      121 gccagaccgc gtcgatcgcg cggtgggcct ctccggtcgt catggcggcc gagtagacca
      181 cctcgggggc gtggcgcaag ccgcggcacc cgtgcgaatg gcgcaaaccg gcgtcgcggg
      241 gggcgagata cgtggtacgg ctatcttcgt cggaactcgc cgtccggatc gcgcccgcgt
      301 gaagaagaac gatgccaaca aggccaacct ggtgccgcgg atcctgccgc gcggccgcca
      361 caagctggat ccccagctgg tggcggcctc gcagcgccag cgcctcctgg aggcgatcgt
      421 cgagctggtc gccgagaagg gctacccgga agtcacgatc ggcgacatcg tgtcgcgggc
      481 cgggacggcc aagcgcacct tctacgacca cttcgccgac aagctggagt gcttcctcgc
      541 cgcgctggag ggcatcaccg acacgctggt cgcggccagc gcgcggctgt tcgcggtctc
      601 gggcacggtg cgcgagcgct gcgagtactc gatgcgcggc tacctggagc tcttggtctc
      661 gatgccgaac accgcgaagg tgttctacct cgaggcggtc gcggccggtc cggagtcggt
      721 gacccgccgc cacgccgtcc acctcaagtt cgcccgcaac atcgtcgggc tgagccgggc
      781 ggcggcggcg gccggggagg gccaggagct gaccgagctc cacgcgctgg ccgtggtcgg
      841 cgccatgcac cagatgatct acggccagct gctcgaccac ggccccgaca gcctgctgga
      901 gatcatcgac gacgtcgtcg agatcgcggt cgcgttcctg accgtgcgga tgccgggggc
      961 gcccacccgc cggccgccgg cgcggcgcgc gcaaaaacgg gataccccgg ccgcgcgacc
     1021 cgacgcgacc cgacgggacg gctcgcgatg acgcggtgcg ttcatgtgcg ggcgggtcgt
     1081 tctgcccccc tgttccgccc ccgggcgccg tccggatccc ccggccgttg aatgactgtg
     1141 ttgcgtgccc tcgggaactt gacagttccc gacggcatct ctaaccatcg ggtggccatc
     1201 gccggagggg caccaccgtg gggtcacggc atgcgaatgc cgtgattggc aatgatgggg
     1261 gcggtgttca caaaccaacg atcgggagac taactatgaa acgtccgagc cgtggttata
     1321 tcgtgttctt gctcgcgggt gtgctgggtg gagtgggggg caccgcatgg gccgaggatc
     1381 ctgccccatc gggcggcgcc gcgccggccg cgccgatggc gatgccgcgc aagttcaccg
     1441 gcgatcagca ggcgttcttc atcgccggcg gcgtggccga cagcacgacc tggacgatcg
     1501 gcaccacgct ccccgcgggg gtgacgatct cggtcggcct gaacatcaac tacaacggca
     1561 acggactgcc gggccccacc gggatgccga gcagcgacaa gttcgcgttc tcgggcgtcc
     1621 tgtacggcgc gtattacatc gtgaacaagt tcccggtcgg catcgccgtc gaggccgcgc
     1681 tggtcgagcc gctgtcgccc acggcgggca gccccgccac cgtcgtccag ccgggtatcg
     1741 gcatcttcta cgcgccgttc cctgcgccga tcgtgctcgg ctcgggtctc gacctgcaga
     1801 tcgtgattcc caagaacggg agcacggtcg tccagaccgt gacgccgggc ctccgcatcg
     1861 tctacgtgtt ttgagtggtc tgatccaggg tcgaggctgc gcggcagcgc acatcgcggc
     1921 cgccgcgccc cggtgatcgc tgcgcgcgcg gatctcgcag catcgcgggg ccggcgcaag
     1981 ggctgcgcgc ttccccgacg ggccaggcgc cggcgctgga acacgggttg ctgagatccg
     2041 gggtatgccg
//
LOCUS       DASVOR010002158         4557 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_315653,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002158 DASVOR010000000
VERSION     DASVOR010002158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4557
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315653"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..467)
                     /gene="secG"
                     /locus_tag="VF516_40015"
     CDS             complement(<1..467)
                     /gene="secG"
                     /locus_tag="VF516_40015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEX8114006.1"
                     /translation="MSTLITILHVIVCLFLMLTVLLQSGKGGGMGAAFGGGNAATVFG
                     GSGASTFLRRLTAGAGTIFILTSMLLAFLASHNSADALEKFGEQQEKLAAEKEAAKKK
                     AMEAGPGSGSSAGSASSSGSASSSSMMTPFAPDQGSAPGPGPGAAGAPAAPAE"
     gene            complement(510..1355)
                     /locus_tag="VF516_40020"
     CDS             complement(510..1355)
                     /locus_tag="VF516_40020"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEX8114007.1"
                     /translation="MRTPFVVGNWKLHKTIAESLALVTELKNQLGAVKGVAVGVAPVF
                     TAIHAVARRLEGSPIATCGQDCHWESRGAWTGEVSADLLADAGASWVIVGHSERRQFF
                     GETSETVGKKARAALAAGLGAIVCVGESLAERDAGRTLAVVDDQLAGALAGIDEPRDA
                     TRGGVNRPASPGERGRESPLSIDEPSAARLVIAYEPVWAIGTGRTATPAQAQEVHAHI
                     RRWLAERLGAGTAGAIRIQYGGSVKPGNAEALMAEADIDGALVGGASLEAADFVAIVK
                     AARPG"
     gene            complement(1359..2567)
                     /locus_tag="VF516_40025"
     CDS             complement(1359..2567)
                     /locus_tag="VF516_40025"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEX8114008.1"
                     /translation="MPAGIKAVTDLAVEGKRVFVRVDYNVPLAKPEAGGGARKVSDDA
                     RIRATLPTLRHLIDRGARIVLGSHLGRPDGQARPEYSLEPVALRLAELLELDVTLADE
                     PVGDGARKVVSDLRDGQIAMLENLRFHPGEEANDDPFARTLASYCDVYVNDAFGTAHR
                     AHASTAGMVKHVAEKGAGFVMAREIDFLSRLLGEVDRPYVAVLGGAKVSDKIEVLDAL
                     LERVNAIVIGGAMANTFLEAQGKALGKSKVERDKLPIARNFLRKAAEKRVAVHLPVDA
                     VVASGVDADAATAVSVDAIPADAMALDIGPATIDAFRKVLAPARTVFWNGPMGVFEKP
                     RFAAGTVAVARALADNKLALTVVGGGDSAAAIAQAGLADKVSHVSTGGGASLEFVQGL
                     ELPGIAALKG"
     gene            complement(2570..3577)
                     /gene="gap"
                     /locus_tag="VF516_40030"
     CDS             complement(2570..3577)
                     /gene="gap"
                     /locus_tag="VF516_40030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEX8114009.1"
                     /translation="MAKVRIGINGFGRIGRGFVRCLAATPDVFDLVLVNDLTDVGTLA
                     HLLKHDSVHGRYPGTVEIKDGAIVVNGQAVKISAEKDPAAIKWRDAGVDIVVESTGKF
                     VDKDGAAKHRASGAKKVVISAPAKNPDVTLCMGINLDMYKPAEHHIISNASCTTNCLA
                     PVAKVLHESFGIVSGLMTTIHSYTNDQQILDLPHKDLRRARAAALSMIPTTTGAAKAL
                     KEVLPAMAGKLDGMAIRVPTPNVSLVDLTARLERKATAHEVNHAFRAAAAGPLKGILA
                     VSDEPLVSCDFNGDLHSSTVDAPLTTATGDLVKVMAWYDNEMGYSQRLLDLTRFIAEK
                     L"
     gene            complement(3741..3914)
                     /locus_tag="VF516_40035"
     CDS             complement(3741..3914)
                     /locus_tag="VF516_40035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114010.1"
                     /translation="MTEREAKSRAVKILAKSIYRDLEAQGFDEKQIVSLATELISEVT
                     AKMSRGDKAQQVA"
     gene            complement(4139..>4557)
                     /locus_tag="VF516_40040"
     CDS             complement(4139..>4557)
                     /locus_tag="VF516_40040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8114011.1"
                     /translation="REPIPEEIAERMELPLDKVRKVLKIAKEPISLETPIGEEEDSHL
                     GDFIEDKSIVSPSESVISVNLADQTRKVLATLTPREEKVLRMRFGIGEKSDHTLEEVG
                     QDFEVTRERIRQIEAKALRKLRHPSRSKRLKAFVEN"
BASE COUNT          631 a         1588 c         1604 g          734 t
ORIGIN      
        1 ctctcggcgg gcgcggccgg ggcgccggca gcgccggggc cggggcctgg ggcgctgccc
       61 tggtccggcg cgaacggggt catcatcgac gacgacgagg cagagcccga gctgctcgcg
      121 ctgccggccg acgacccgga gccggggccg gcctccatcg ccttcttctt ggccgcttcc
      181 ttctcggctg cgagcttctc ctgctgctcg ccgaacttct cgagggcatc ggccgagttg
      241 tggctggcga ggaacgcgag cagcatcgag gtcaggatga agatcgtgcc ggcgcccgcg
      301 gtcagccggc ggaggaacgt cgacgcgccc gagccgccga acaccgtcgc ggcgttgccg
      361 ccgccgaacg ccgcgcccat gccgccgccc ttgccgctct gcagcagcac cgtgagcatc
      421 aggaacaggc acacgatgac gtgcaggatg gtgatcagcg tcgacatgcg gaacttgctt
      481 ctagcccaac ccgagggggc gtgcaagcct cagcccggcc gcgctgcctt gacgatcgca
      541 acaaaatcag cggcttccag cgaggccccg ccgaccaggg cgccatcgat gtcggcctcg
      601 gccatcagcg cctcggcgtt gcccggcttg accgacccgc cgtactggat gcggatcgcg
      661 ccggcggtcc cggcgccgag ccgctcggcg agccaccgcc ggatgtgggc gtggacctcc
      721 tgggcctggg cgggggtcgc ggtgcggccg gtgccgatcg cccacaccgg ctcgtaggcg
      781 atcacgagcc gggcagcgct tggctcatcg atcgagaggg ggctttcccg ccccctctcg
      841 cccggcgaag ccgggcgatt cacccctccg cgcgtcgcgt cgcgcggctc gtcaataccg
      901 gcgagcgcgc cggcgagctg gtcgtcgacg accgccagcg tccggccggc gtcgcgctcg
      961 gcgagcgact cgccgacgca gacgatcgcg cccaggcccg cggcgagcgc cgcgcgcgcc
     1021 ttcttgccca ccgtctcgct ggtctcgccg aagaactgcc ggcgctcgct gtggccgacg
     1081 atcacccagc tcgccccggc gtcggcgagc aggtcggccg agacctcgcc ggtccacgcg
     1141 ccccgggact cccagtggca gtcctggccg caggtcgcga tcggcgagcc ctcgagccgc
     1201 ctggcgacgg cgtggatcgc ggtgaacacc ggcgcgacgc cgaccgcgac gcccttgacc
     1261 gcgccgagct ggttcttgag ctcggtgacc agggcgagcg attcggcgat cgtcttgtgg
     1321 agcttccagt tgccgacgac gaacggggtg cgcatggttc aacccttgag cgccgcgatg
     1381 cccggaagct cgaggccctg gacgaactcc agcgacgcgc cgccgccggt cgacacgtgc
     1441 gagaccttgt cggcgagccc ggcctgggcg atcgcggccg ccgagtcgcc gccgccgacg
     1501 acggtgagcg cgagcttgtt gtcggcgagc gcgcgcgcca cggcgacggt gccggccgcg
     1561 aaccgcggct tctcgaacac gcccatcggg ccgttccaga acaccgtgcg ggcgggggcg
     1621 agcaccttgc ggaacgcgtc gatcgtcgcc gggccgatgt cgagcgccat cgcgtcggcg
     1681 gggatcgcat cgaccgatac ggccgtcgcg gcgtcggcgt cgacgccgga cgcgacgacc
     1741 gcgtcgaccg gcaggtggac cgcgacgcgc ttctcggctg ccttgcgcag gaagttgcgc
     1801 gcgatcggca gcttgtcgcg ctcgaccttg ctcttgccca gcgccttgcc ctgggcctcg
     1861 aggaacgtgt tggccatcgc gccgccgatc acgatcgcgt tgacccgctc gagcagggcg
     1921 tcgagcacct cgatcttgtc cgacaccttg gcgccgccga gcaccgcgac gtacggccgg
     1981 tcgacctcgc cgagcagccg cgacaggaag tcgatctccc tggccatcac gaagccggcg
     2041 cccttctcgg cgacgtgctt gaccatcccg gcggtcgacg cgtgggcacg gtgcgcggtg
     2101 ccgaacgcat cgttgacgta gacgtcgcag tacgacgcga gcgtgcgcgc gaacgggtcg
     2161 tcgttggcct cctcgccggg gtggaaccgc aggttctcca gcatcgcgat ctggccgtcg
     2221 cgcaggtcgc tgaccacctt gcgcgcgccg tcgccgaccg gctcgtcggc gagcgtgacg
     2281 tcgagctcga gcaactcggc gagccgcagc gcgaccggct cgagcgagta ctccggcctg
     2341 gcctggccgt cgggccggcc gaggtgcgag ccgagcacga tccgcgcgcc gcgatcgatc
     2401 aggtggcgca gggtgggcag cgtggcccgg atccgggcgt cgtcgctgac cttgcgcgcg
     2461 ccgccgcccg cctccggctt ggcgagcggg acgttgtagt cgacccggac gaacacgcgc
     2521 ttgccctcga ccgcgaggtc ggtgacggcc ttgatccccg ccggcaaagt cacagcttct
     2581 ccgcgatgaa ccgggtcagg tcgagcaggc gctgcgagta gcccatctcg ttgtcgtacc
     2641 aggccatcac cttgaccagg tcgccggtcg cggtggtcag cggggcgtcg accgtcgacg
     2701 agtgcaggtc gccgttgaag tcgcacgaca ccagcggctc gtcgctgacc gcgaggatgc
     2761 ccttgagcgg gccggcggcc gcggcgcgga acgcgtggtt gacctcgtgc gcggtggcct
     2821 tgcgctcgag ccgcgcggtg aggtcgacca gcgagacgtt gggcgtcggg acccggatcg
     2881 ccatgccgtc gagcttgccg gccatcgcgg gtagcacctc cttgagcgcc ttggccgcgc
     2941 cggtcgtggt cgggatcatc gacagcgcgg ccgcgcgggc gcgccgcagg tccttgtggg
     3001 gcaggtcgag gatctgctgg tcgttggtgt acgagtggat cgtggtcatc agccccgaca
     3061 cgatgccgaa gctctcgtgc aggaccttgg cgaccggcgc caggcagttg gtcgtgcacg
     3121 acgcgttgga gatgatgtgg tgctccgccg gcttgtacat gtccaggttg atgcccatgc
     3181 acagcgtgac gtcggggttc ttggccgggg ccgagatcac gaccttcttg gcgccgctcg
     3241 cgcggtgctt ggccgcgccg tccttgtcga cgaacttgcc ggtcgactcg acgacgatgt
     3301 cgacgcccgc gtcgcgccac ttgatcgcgg ccgggtcctt ctcggcgctg atcttcacgg
     3361 cctggccgtt gaccacgatc gcgccgtcct tgatctcgac ggtcccgggg taccggccgt
     3421 gcaccgagtc gtgcttgagc aggtgcgcga gcgtgccgac gtcggtcagg tcgttgacca
     3481 ggacgaggtc gaacacgtcg ggagtggccg cgaggcagcg cacgaagccg cgtccgatcc
     3541 gaccgaatcc gttgataccg atgcgaacct tcgccatatg gcgcgcaccc tagatcggat
     3601 cgtcgagcgc tgccagcgtc ctgggggcgc agatcgagca ccggatcgcg aggtcaccgt
     3661 cacgagctcc cgccccggcg cgccacgagg tgaaagaccg cagaccgtgc gttcacgccc
     3721 aaatttttcg cgggcgcgcg ctaggcgacc tgctgcgcct tgtcgccgcg cgacatcttg
     3781 gccgtgacct cgctgatcaa ctcggtggcc agcgatacga tctgcttctc gtcaaagccc
     3841 tgcgcctcga ggtcgcggta gatcgacttg gcgaggatct tcaccgctcg ggacttggct
     3901 tcgcgctcgg tcatggtcag tccgaatcac aaagcactga ctgtgccaga tcatgcccag
     3961 tcgtcatgcg tggcaaaccg tgagaatcac gagatagctc cagaggagga tcttgtaaga
     4021 tcttctcacc gcgaagcact ctcctgcata gccgaggaat cggtgcataa cgcgctacgc
     4081 acacggtcgg accattcggc cgtgtgcgcc cagcgcgttg tttcgcgcgg gtttcggcct
     4141 agttctcgac gaaggccttg agtcgcttgg agcgcgaggg gtgacgcagc ttgcggagcg
     4201 ccttggcctc gatctggcgg atgcgctcgc gggtgacctc gaagtcctgg ccgacctcct
     4261 cgagcgtgtg gtctgacttc tcgccgatgc cgaaccgcat gcgcaggacc ttctcctcgc
     4321 gcggggtgag cgtggcgagc accttgcggg tctgatccgc gaggttcacc gagatcacgc
     4381 tttcgctcgg cgagacgatc gacttgtcct cgatgaagtc gccgaggtgg gagtcttcct
     4441 cctcgccgat cggggtctcc agcgagatcg gctccttggc gatcttgagg accttgcgga
     4501 ccttgtcgag cggcagctcc atgcgctcgg cgatctcctc ggggatcggc tcgcggc
//
LOCUS       DASVOR010002159         6371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_461323,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002159 DASVOR010000000
VERSION     DASVOR010002159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461323"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VF516_40045"
     CDS             <1..355
                     /locus_tag="VF516_40045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114012.1"
                     /translation="PRVASPPAVLPAAIDPAELAALDDLLDQGRLDDALSGYRMLARR
                     HAHDRHVRAGIELCGGLLALAAHDRREAAQRLETALEIDPSNERAARELAEMRRQATH
                     ERKGLLSRLMGKKEP"
     gene            360..2027
                     /locus_tag="VF516_40050"
     CDS             360..2027
                     /locus_tag="VF516_40050"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8114013.1"
                     /translation="MSRIIGIDLGTTNSCAAVLDDKGQPRILSGADGERIVPSWVSWA
                     ESGQVAVGARARRQGVTNAAATVFGAKRLIGRKVNADDVSWFARLAPFRIVAAPNGDA
                     WVRIHGQPISPQEIASHILRAVRKVAEDALGEPVTRAVVTVPAYFDDAQRQATRDAGQ
                     IAGLDVVRILNEPTAAALAYGAHRGSGGRRVIAVFDLGGGTFDISIMSVDHGIFEVLA
                     TGGDSALGGEDWDRRVLERLVDEVSDRHRIDLTAIPVAMSRLRDACEAAKQALSVDRE
                     ARINLPFLANDAHGAPINYERTVTRELFETATRDLLDRLEPPCRRALADARLTAADVE
                     DVLLVGGMTRWPAVAEVVERVFHRKPSRGANPDEVVALGAAQYAGILAGTSDDAALLD
                     VTPHDLGIKVGDSQFAVVLPRNSMLPVRARRVFKTTEDNQRFVTIELYQGDSPEIANN
                     RKLGQVVLDDLPPGPAGATRVELVMTANVESILSVTARELKTGRAASVTIRPSGGLSQ
                     REIIEIINRRRQEHAALGVPPEPVGGAVRVTTRDQVVTKPDDPDGVP"
     gene            2024..2581
                     /locus_tag="VF516_40055"
     CDS             2024..2581
                     /locus_tag="VF516_40055"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEX8114014.1"
                     /translation="MTVDPAVLKVADAVGALIEAWGFRRNMGRMWAVLYLEDHPLTAA
                     DLGERLGLSTGAVSMLVNELIQWGIIKKTWVVGERREHFEAETSIWKMISRVFREREL
                     RWIRTAHEAFAEASRELEAGTEVENDARHQLIASRVAGLTQLAEVGAHLLQTMLEGEA
                     IDSLPIKQVGELAAAARQDTDPDKP"
     gene            2578..3594
                     /locus_tag="VF516_40060"
     CDS             2578..3594
                     /locus_tag="VF516_40060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8114015.1"
                     /translation="MIRARDLTKHFRVHKRPPGFASALRSLVHRRYEVVKAVDGVSFT
                     IERGERVGFLGPNGAGKTTTLKVLSGLLHPTSGEVSVEDCVPFRRQASFLGKITLVMG
                     QKSQLIWDLPPAETYAMNRAVFDVPRDRFERTLAELTELLELGPLLDKPTRQLSLGER
                     MKCELAAALLHQPTTLFLDEPTIGLDVAMQVAIRDFVRSYNQRHEATVLLTSHYMDDV
                     VALCPRIIVIDQGKLIHDGDLRQLIKTRDPDKRVSFTLTRAIGDDELARLGALLTRDG
                     QRITLRVAEREVPGVIGRLLGALGAADLAIEDPPLEDVLRAMFGEQRRAEAEAKAETG
                     AEAS"
     gene            3591..4391
                     /locus_tag="VF516_40065"
     CDS             3591..4391
                     /locus_tag="VF516_40065"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX8114016.1"
                     /translation="MTVARTLRALPTLLRIGVAETLAYRAEFLVWILTTTQPLIMMGL
                     WTSVARLQAFAHYSSADFVAYFLSSLIVRQLTGNWVAWQISEEVRSGTMAMRLLRPIH
                     PFVAYASSQAAAIPFRSIIAVPFALILLASSGSGALTTDPARLALIVPSIALAWLITF
                     ALQFAIGALAFFLTQTMAIVNLYFALYSLLSGYLMPLALLGPVGELARWLPFRFMLSA
                     PVELMTAAPDGDDPVQVLGAQAAWAAISLAGALVLWRRGVRRFEAVGG"
     gene            4388..5179
                     /locus_tag="VF516_40070"
     CDS             4388..5179
                     /locus_tag="VF516_40070"
                     /inference="COORDINATES: protein motif:HMM:NF017946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEX8114017.1"
                     /translation="MIRLLRLFALQFRVSAASAMAYRANFLIQGAMSAAWTVLNLVPL
                     IVVFGHSDKIAEDWDRPSALIVMAYFTGVHAVIEGTISPSMQDLVEKIRSGAFDYVLL
                     KPVDAQAMISTSRYEPWRVFDLLAAIAIAVYALIQRGEAPSAIDIALGIALFGAGVLA
                     TYALWMVCAAASFWVVRLDNLMFLLGSIFDVARWPVDVFQGVWRFVFTFVIPVAVMTT
                     YPARALLGRLGPARTLATAGGALLLLVLSRVIWRTAIRSYTSASS"
     gene            complement(5951..6073)
                     /locus_tag="VF516_40075"
     CDS             complement(5951..6073)
                     /locus_tag="VF516_40075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114018.1"
                     /translation="MPAIPAPPGAHRAHHPHERHIHVPGKGDVDVKWDVDVNWE"
BASE COUNT          892 a         2254 c         2308 g          917 t
ORIGIN      
        1 tccacgggtg gcttcgcccc ccgcggtgct gcccgcggcg atcgatcccg ccgagctcgc
       61 cgcgctggac gacctgctcg accaggggcg gctcgatgac gcgctctcgg gctaccggat
      121 gctcgccagg cgccacgccc acgaccgcca cgtccgcgcc gggatcgagc tgtgcggagg
      181 cctgctcgcg ctcgccgcac acgaccggcg ggaggccgcg cagcggctgg agaccgcgct
      241 cgagatcgat ccgtcgaacg agcgcgccgc gcgcgagctc gccgagatgc ggcgccaggc
      301 cacccacgag cgcaagggct tgctcagccg cctgatgggc aagaaggagc cgtaggcgga
      361 tgtcacgcat catcggcatc gatctcggaa ccaccaactc gtgcgccgcc gtgctcgacg
      421 acaagggcca gccccggatc ctgtccgggg ccgacggcga gcggatcgtc ccgtcgtggg
      481 tcagctgggc ggagtccggc caggtcgcgg tcggcgcgcg cgcccgccgc cagggcgtga
      541 ccaacgccgc ggcgaccgtg ttcggcgcca agcggctgat cggccgcaag gtcaacgccg
      601 acgacgtgtc gtggttcgcc cggctcgcgc cgttccggat cgtcgcggcg cccaacggcg
      661 acgcctgggt ccgcatccac ggccagccga tctcgcccca ggagatcgcg tcccacatcc
      721 tgcgcgcggt gcgcaaggtc gccgaggacg cgctcggcga gccggtgacc cgcgcggtgg
      781 tgaccgtccc ggcctacttc gacgacgccc agcgccaggc gacccgcgac gccggccaga
      841 tcgccgggct cgacgtggtc cggatcctca acgagccgac cgccgccgcg ctggcctacg
      901 gcgcgcaccg ggggtcgggc ggccgccggg tgatcgcggt gttcgacctc ggcggcggca
      961 cgttcgacat ctcgatcatg agcgtcgacc acgggatctt cgaggtcctg gcgaccggcg
     1021 gcgacagcgc gctcggcggc gaggactggg atcgccgcgt gctcgagcgc ctggtcgacg
     1081 aggtgtcgga tcgccaccgg atcgacctca cggcgatccc ggtcgcgatg tcgcggctgc
     1141 gcgacgcctg cgaggccgcc aagcaggcgc tgtcggtcga ccgcgaggcc cggatcaacc
     1201 tgccgttcct ggccaacgac gcgcacggcg cgccgatcaa ctacgagcgc acggtgaccc
     1261 gcgagctgtt cgagaccgcg acccgcgatc tgctcgaccg gctcgagccg ccgtgccgcc
     1321 gcgcgctcgc cgatgcccgg ctgaccgcgg ccgacgtcga ggacgtcctc ctggtcggcg
     1381 ggatgacccg gtggccggcg gtggccgagg tcgtcgagcg cgtgttccac cgcaagccgt
     1441 cgcgcggcgc caaccccgac gaggtcgtcg cgctcggcgc cgcgcagtac gccggcatcc
     1501 tcgccggcac ctccgacgac gccgcgctgc tcgacgtcac gccgcacgac ctcggcatca
     1561 aggtcggcga ctcgcagttc gcggtggtcc tgccgcgcaa ctcgatgctg ccggtgcgcg
     1621 cccggcgggt gttcaagacc accgaggaca accagcggtt cgtgacgatc gagctctacc
     1681 agggcgactc acccgagatc gcgaacaacc gcaagctcgg ccaggtcgtg ctcgacgacc
     1741 tgccgcccgg cccggccggc gcgacccggg tcgagctggt gatgacggcc aacgtcgagt
     1801 cgatcctgtc ggtgaccgcg cgcgagctca agaccggccg ggccgccagc gtcacgatcc
     1861 ggccgtccgg cgggctgtcg cagcgcgaga tcatcgagat catcaaccgg cggcgccagg
     1921 agcacgcggc gctcggcgtg ccgcccgagc cggtcggcgg cgcggtgcgc gtcaccaccc
     1981 gcgaccaggt cgtgaccaag cccgacgacc ccgacggcgt gccatgaccg tggatcccgc
     2041 cgtgctcaag gtcgccgatg cggtcggggc gctgatcgag gcgtggggct tccgccgcaa
     2101 catgggccgg atgtgggccg tgctctacct cgaggaccac ccgctgacgg cagccgacct
     2161 cggcgagcgg ctcgggctgt cgaccggcgc ggtgtcgatg ctggtcaacg agctgatcca
     2221 gtgggggatc atcaagaaga cctgggtggt cggcgagcgc cgcgagcact tcgaggccga
     2281 gaccagcatc tggaagatga tctcgcgggt gttccgcgag cgcgagctgc gctggatccg
     2341 caccgcgcac gaggcgttcg ccgaggccag ccgcgagctc gaggccggca ccgaagtgga
     2401 aaacgacgcg cgccaccagc tgatcgcgag ccgggtcgcc gggctgaccc agctcgccga
     2461 ggtcggcgcc cacctgttgc agaccatgct ggagggcgag gcgatcgact cgctgccgat
     2521 caagcaggtc ggcgagctcg ccgccgccgc ccgccaggac accgacccgg acaagccgtg
     2581 atccgcgccc gcgacctgac caagcacttc cgggtccaca agcggccgcc cggcttcgcg
     2641 tccgcgctgc gctcgctggt ccaccgccgc tacgaggtcg tcaaggcggt cgacggcgtc
     2701 agcttcacga tcgagcgcgg cgagcgggtc ggcttcctcg ggcccaacgg tgcgggcaag
     2761 accacgacgc tcaaggtgct gtccggcctg ctccacccga ccagcggcga ggtctcggtc
     2821 gaggactgcg tgccgtttcg ccgccaggcc agcttcctgg gcaagatcac gctggtcatg
     2881 ggccagaagt cgcagctgat ctgggacctg ccgccggccg agacctacgc gatgaaccgc
     2941 gcggtgttcg acgtgcccag ggaccgcttc gagcgcacgc tcgccgagct gaccgagctg
     3001 ctcgagctcg gcccgctgct cgacaagccg acccgccagc tgtcgctcgg cgagcgcatg
     3061 aagtgcgagc tcgccgccgc gctgctccac cagccgacca cgctgttcct cgacgagccg
     3121 accatcgggc tcgacgtcgc gatgcaggtc gcgatccgcg acttcgtgcg cagctacaac
     3181 cagcgccacg aggccacggt gctgctcacc tcgcactaca tggacgacgt cgtcgcgctg
     3241 tgcccgcgga tcatcgtgat cgaccagggc aagctgatcc acgacggcga cctccgccag
     3301 ctgatcaaga cgcgcgaccc cgacaagcgg gtgtcgttca cgctgacccg ggcgatcggc
     3361 gacgacgagc tcgcccggct cggcgcgctg ctcacccgcg acggccagcg catcacgctg
     3421 cgggtcgccg agcgcgaggt ccccggcgtg atcggccggc tgctcggcgc actcggcgcc
     3481 gccgatctgg cgatcgagga cccgccgctc gaggacgtgc tgcgcgcgat gttcggcgag
     3541 cagcgccgcg cggaggccga ggccaaggcc gagaccggag ccgaggcgtc atgaccgtcg
     3601 cgcgcacgct gcgcgcgctg ccgacgctgt tgcggatcgg cgtcgccgag accctggcgt
     3661 accgcgccga gttcctggtc tggatcctga ccacgaccca gccgctgatc atgatggggc
     3721 tgtggaccag cgtcgcgcgg ctccaggcgt tcgcgcacta ctcgtcggcc gacttcgtcg
     3781 cctacttcct gtccagcctg atcgtccgcc agctgaccgg caactgggtc gcgtggcaga
     3841 tcagcgagga ggtgcggtcc ggcacgatgg cgatgcggct gctccgcccg atccacccgt
     3901 tcgtcgccta cgcgtcgagc caggccgcgg cgatcccgtt ccgctcgatc atcgccgtgc
     3961 cgttcgcgct gatcctgctg gccagctcgg gcagcggtgc gctgaccacc gacccggccc
     4021 ggctcgcgct gatcgtcccg tcgatcgcgc tggcctggct gatcacgttc gccctgcagt
     4081 tcgcgatcgg ggcgctcgcg ttcttcctga cccagaccat ggcgatcgtc aacctgtact
     4141 tcgcgctgta ctcgctgctc tccgggtacc tgatgccgct ggccctgctc gggccggtcg
     4201 gcgagctcgc gcgctggctg ccgttccggt tcatgctgag cgcgccggtc gagctgatga
     4261 ccgccgcgcc ggacggcgac gacccggtcc aggtgctcgg cgctcaggcc gcgtgggccg
     4321 cgatctcgct cgccggcgcc ctggtgctgt ggcggcgcgg cgtgcggcgg ttcgaggcgg
     4381 tgggtggatg atccgcctgc tgcggctgtt cgcgctccag ttccgggtca gcgccgcgtc
     4441 ggcgatggcc tatcgcgcca acttcctgat ccagggcgcg atgtcggcgg cgtggacggt
     4501 gctcaacctc gtgccgctga tcgtggtgtt cggccacagc gacaagatcg ccgaggactg
     4561 ggaccggccg tctgcgctga tcgtcatggc gtacttcacc ggcgtgcacg cggtgatcga
     4621 gggcacgatc agcccgtcga tgcaggacct ggtcgagaag atccgctccg gcgcgttcga
     4681 ctatgtcctg ctcaagccgg tcgacgccca ggcgatgatc tcgacgtcgc gctacgagcc
     4741 gtggcgggtg ttcgatctgc tcgccgcgat cgcgatcgcc gtctacgcgc tgatccagcg
     4801 cggcgaggcc ccgtcggcga tcgacatcgc gctcggcatc gcgctgttcg gcgccggcgt
     4861 gctcgcgacc tacgcgctgt ggatggtctg cgccgccgcc tcgttctggg tcgtccggct
     4921 cgacaacctc atgttcctgc tcggctcgat cttcgatgtc gcgcgctggc cggtcgatgt
     4981 gttccagggc gtgtggcggt tcgtcttcac cttcgtgatc ccggtggcgg tgatgaccac
     5041 gtaccccgcg agggcgctgc tcggccggct cgggcccgcc cggacgctcg ccaccgccgg
     5101 cggtgcgctg ctgctgctcg tgctcagccg ggtgatctgg cgcacagcga tccgcagcta
     5161 caccagcgca tccagctgat ctcgcgtctt cccgcgccgg gcgggggccg gtcgattctt
     5221 gggaagaatc gtccggtgac ccgtcatacg ggtagcaggt ccgggggtgc acacgcaccg
     5281 tgtcccggcg gaaggcgaat gatcgagggc gggtagcgag atacggaggg cgggccaggg
     5341 cgagcgcagg gcggcggcgc gtgcagggga agtgctggga gacgtggaag atggcgagcg
     5401 aggtgctcgt cgggcgcgcg gaaggggcgg tggcggggcc gacggggcgg cggggtcccg
     5461 aaacgaggtc ggaggacggg caacggaggc gggaaaccag ggcaggcggc ggttcggggg
     5521 agccgccgcc ccctgggagg gggatggtcc gcgcggcgtc gcgagcgtgc gaccctggcg
     5581 gcgcccgcga cagctggctc tgatgattcg tcacaggtct tcgatatggc gttcgtgatc
     5641 gtcgacgttc gtcgacgtcg atctgatcgt tgacccagag cgttgaggtc gacggcgacg
     5701 tacgacgttg agcccaccat cgacgttgga cgtcgaccga tcctgtgctg cgagcctcga
     5761 cggagcgcga tagcaggtcg aggtcgacgg tggggtcaga cgtctacgta tcgcttcgct
     5821 gccgtcaagc acaacgctcg agtcaaggtc gacgtcatcg tcgacgtcat cgtcgagcgc
     5881 cacgggaacg gccacgagcg cgcgacgttt gtgatgcatc atcagacagc gggccagagc
     5941 cgggtggtga tcactcccag ttgacgtcga cgtcccactt cacgtcgacg tcgcccttgc
     6001 cgggcacgtg gatgtggcgc tcgtggggat gatgcgcccg gtgcgctcca ggtggagccg
     6061 ggatcgcagg cacagccgga accgcaggca ccgccggaac cgcaggcacc gccggaaccg
     6121 caggcaccgc cggaaccgcg ggcaccgccg ggaccgcggg caccgccggg accgcaggca
     6181 ccgccgggac ggcgaccgcc gggacggcga ccgccggaat cgagggattg atgcgtatgc
     6241 gtggcgcgat gcgggccatc ggcggcatcg gcgacatggg aggcatcggc ggcatgggag
     6301 gcatcggcgg catcgggccg atcagtgggc gtgcgtgcgg atgtggatgt ggatgagggt
     6361 gcggccgcac g
//
LOCUS       DASVOR010002160         2303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_373931,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002160 DASVOR010000000
VERSION     DASVOR010002160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373931"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VF516_40080"
     CDS             <1..214
                     /locus_tag="VF516_40080"
                     /inference="COORDINATES: protein motif:HMM:NF020544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HEX8114019.1"
                     /translation="DVAEVGAALATVTHDWFAGRFATLRQHGYSGAGDERDLAYTWHW
                     FEQMRAFFARTAAEKRAVMFSADTLL"
     gene            complement(579..1043)
                     /gene="ybeY"
                     /locus_tag="VF516_40085"
     CDS             complement(579..1043)
                     /gene="ybeY"
                     /locus_tag="VF516_40085"
                     /inference="COORDINATES: protein motif:HMM:NF014216.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEX8114020.1"
                     /translation="MTLDLAVDDAVRLPSATRRRLRTEVARMIRAAARHDGRADYEVS
                     LRLVGDVQIHALNRGYRRKDRPTDVLAFAQREGPAGALHPGLLGDIVISVDTARRQAR
                     HGLYRELLHLASHGLCHLLGYDHRTDAEEADMNARAAALRAEARRRGRIRPA"
     gene            complement(1051..>2303)
                     /locus_tag="VF516_40090"
     CDS             complement(1051..>2303)
                     /locus_tag="VF516_40090"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEX8114021.1"
                     /translation="NPWGFTHDRSFDDQNRYQTRSVLAVPMISARNEVIGVIQLINKR
                     AKDWIQLDLPSDFEDGVVPFDDISETYIFALASQAGIALENVLLYGEVKTLFEGFVKA
                     AVTAIESRDPTTSGHSERVAQLTVNLAMSVNQTDTGHFREVRFSRDDLIQIEYAGLLH
                     DFGKVGVREQVLVKAKKLYEHERELILQRFQLIKRGYKIEGLESKVRYLLEASREQVA
                     AQLAAVDAEIGRKVSELDDIIRFILASNEPTVLDHGAFERIAEIAALSWRDDDHHQLP
                     YLTPNEATCLQIRRGSLTEQEREKINEHVNHTYRFLKQIPWSRTLRDVPDIARWHHEK
                     LDGTGYPHQLRGEQIPIPSRMMAISDIYDALTAADRPYKRAMPTEKALDILAADVKRG
                     QLDAHLFEVFVEARVWTLPPPRLS"
BASE COUNT          332 a          787 c          794 g          390 t
ORIGIN      
        1 tgacgtcgcg gaggtcgggg ccgcgctggc gacggtcacc cacgactggt tcgcgggccg
       61 ctttgcgacg ctcagacaac acggctactc cggggcgggg gatgaacgcg acctcgcgta
      121 cacttggcac tggttcgagc aaatgcgcgc gttcttcgct cggactgcag cggagaagcg
      181 cgccgtgatg ttcagcgccg acacgttgct ttgaaggcag cgagatcgac atagtgaacc
      241 cgatcgccga tgccgccgcg cagctcaccg ccgtggtact ggttgctggc cgcgcgaacc
      301 agggcgcgca cgctggacgt cgcggtcggc cgcgctctta ggtgccggcc agcggttcga
      361 catggacggc cgccgaactg cacagtccct accgcgtccc tacctcgagc tggtccctac
      421 ctcggtccct acttttctgt ggcgccgtga taggcctgga ttggatacgc ctcgaaactg
      481 ttaacgtatg ggaaccagtc gatctcgaag tagtctcaag ctcgtcggag gtcgtccgag
      541 atccggccgg ggaactcgga cgcctcgata ccacgcgctc acgccgggcg gatccggccc
      601 cgccgcctcg cctcggcccg cagcgccgcc gcccgggcat tcatgtccgc ctcctcggcg
      661 tcggtgcggt ggtcgtagcc gagcaggtgg cacaggccat gggacgccag gtgcaagagc
      721 tcgcggtaca gcccgtggcg ggcctggcgg cgcgccgtgt cgaccgagat cacgatgtcg
      781 ccgagcagcc ccgggtggag cgccccggcc ggcccctcgc gctgcgcgaa cgccagcacg
      841 tcggtcggcc ggtccttgcg ccggtagccg cggttgagcg cgtggatctg cacgtcgccg
      901 accagccgga gcgacacctc gtagtcggcg cgcccgtcgt gccgcgccgc ggcgcggatc
      961 atccgcgcga cctcggtgcg cagccggcgg cgcgtggcgg acggcaggcg gaccgcgtcg
     1021 tcgacggcga ggtcgagcgt cattcgaacc tcaggacagg cggggcggcg gcagcgtcca
     1081 gacccgggcc tcgacgaaca cctcgaacag gtgggcgtcg agctgaccgc gcttgacgtc
     1141 cgccgcgagg atgtcgagcg ccttctcggt cggcatcgcc cgcttgtacg gccggtcggc
     1201 cgcggtcagc gcgtcgtaga tgtcgctgat cgccatcatc cgcgatggga tcgggatctg
     1261 ctcaccgcgc agctggtggg ggtagcccgt gccgtcgagc ttctcgtggt gccagcgcgc
     1321 gatgtccggc acgtcgcgca gcgtgcggct ccacgggatc tgtttcagga aacgatacgt
     1381 gtgattgaca tgctcgttga tcttctcgcg ctcctgctcg gtgagcgagc cgcgccggat
     1441 ctgcaggcag gtcgcctcgt tcggcgtcag gtacggcagc tggtggtggt cgtcgtcgcg
     1501 ccacgacagc gcggcgatct cggcgatccg ctcgaacgcg ccgtggtcga gcacggtcgg
     1561 ctcgttggac gccaggatga accggatgat gtcgtcgagc tcggagacct tgcgcccgat
     1621 ctcggcgtcg accgccgcca gctgcgccgc gacctgctcg cgcgacgcct ccagcaggta
     1681 gcggaccttg gactccaggc cctcgatctt gtagccgcgc ttgatcagct ggaaccgctg
     1741 caggatcagc tcgcgctcgt gctcgtacag cttcttggcc ttgaccagca cctgctcgcg
     1801 cacgccgacc ttgccgaagt cgtgcaagag cccggcgtac tcgatctgga tcaggtcgtc
     1861 gcgcgagaac cggacctcgc ggaagtgccc ggtgtcggtc tggttgaccg acatcgcgag
     1921 gttgaccgtg agctgcgcga cccgctcgga gtggcccgac gtcgtcggat cgcgcgactc
     1981 gatcgccgtg accgccgcct tgacgaagcc ctcgaacagc gtcttgacct cgccgtacag
     2041 cagcacgttc tccagcgcga tccccgcctg cgacgccagc gcgaagatgt aggtctccga
     2101 gatgtcgtcg aacggcacca cgccgtcctc gaagtcgctc ggcaggtcga gctggatcca
     2161 gtccttcgcg cgcttgttga tcagctggat cacgccgatc acctcgttgc gcgccgagat
     2221 catcggcacc gcgagcaccg agcgggtctg gtagcggttc tggtcgtcga acgagcggtc
     2281 gtgggtgaac ccccacgggt tgt
//
LOCUS       DASVOR010002161         4673 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_315667,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002161 DASVOR010000000
VERSION     DASVOR010002161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315667"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..616
                     /locus_tag="VF516_40095"
     CDS             <1..616
                     /locus_tag="VF516_40095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114022.1"
                     /translation="PSASGLADALAGAVTVAGGAAPSAGPSAGLHAGTSTRSSAGAKA
                     QTASGTGADVQPMPGALRWRPWALGAAVVLVLVAIAARGSSPVSAPGAALPRDPSGAQ
                     SLDREDREDRGEGDDRRDLRDIRMTPPAGMDDKQTRDWNKIADALRRGHYEDARHKLR
                     EFEDKYGQTDETRALAPQLDQLGPDDPRGPGGPPGHGRGKKHHD"
     gene            complement(659..1459)
                     /locus_tag="VF516_40100"
     CDS             complement(659..1459)
                     /locus_tag="VF516_40100"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8114023.1"
                     /translation="MRSSTPTTTNGLPRIRIARPTGSKFGPHSFSTTVSPSSATLTLP
                     SMSPAWIARPRAIRQLRIAAPQTARHAQPLRRALSACTHCGVARRWMGPLRRLAALLG
                     VERTVHCYLAMLISMTQTVRIGVVTASQVGLFLAAIAFGTCWKHEPELQADRTVVAPP
                     SKDPATARSLIASGAVVIDVRTADEFAERHLASAVNLPVDELPGRLAEVDRLVGGDRT
                     RPIVVYCAAGARAARAKARLEAAGYTNVVNGGGLDDLRAADDRPPPQG"
     gene            1582..1938
                     /locus_tag="VF516_40105"
     CDS             1582..1938
                     /locus_tag="VF516_40105"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEX8114024.1"
                     /translation="MVSLVVGGIGIMNIMLVSVTERTREIGVRMAVGARSWHILGQFL
                     AEALALSLAGGALGIALGLTIAGQLAARFHMPSMIHWNVIALAVGFSAAVGVGFGLYP
                     ARKASRLDPIDALRFE"
     gene            complement(1988..2419)
                     /locus_tag="VF516_40110"
     CDS             complement(1988..2419)
                     /locus_tag="VF516_40110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114025.1"
                     /translation="MRFLVWLMLGVSGVAHADAPDTLSAKEIQYYFAPYLPEVRSCYT
                     SYGHGRAAAGALRLELIIQPGGSVARLGVTAPGVDPSWLTRLGGCLRQHVRAWHFPAR
                     GSVTTAMMPFLFHKADAPGAGPIESCWDPRGCPPGERETPR"
     gene            complement(2543..4336)
                     /locus_tag="VF516_40115"
     CDS             complement(2543..4336)
                     /locus_tag="VF516_40115"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8114026.1"
                     /translation="MQAPADPASDGADAAATARRAHPGTRTPIQHAIIVVGENHSFDN
                     LFGTYQPRRGQHIDNLLSKGIVNADGSPGPHFARAQQRIGSDTDRYHVETASTGAYDV
                     LPQPFTSGGIGLPDGVPDVRFPANLPNGPFQISKYVPYAAYTGDPVHRFFQMWQDVDG
                     GRMDKFAWVEKTVGIGGNDAPPPAGGFDPLEGAISLGFYNMNPFVNAAGIASPGDAPF
                     FKSLADRFAISDNYHQAIMGGTGANFMALVTGHAAFFTSTTLDGSAATPFANQIENPD
                     PVAGTNNYYTQDGYSGGSYVNCADASAPGVAAVLGELHRQGVRNANCERDHFYLLNNY
                     ALFWNQTRSNPRTLGADQFTLPPQSAPTIADVMTRRGVAWKYYSADRGDDPTVFAQSV
                     DGVPLRFHAYCGICDPLTGFSSIMKNPSEEAKLASYGAFLHDVHAGTLPAVSFVRPFE
                     TLAGHPADSTADLYEQFLADLIAQVQADPELWATTAIFITTDEGGGYYDSGYIQAVDF
                     FGDGTRIPMIVVSPFAKQGFVDHTYYDHVSLLKFIEHNWNLPPVSRRSRDNLPNPVAS
                     RANPYVPVNGPAIGDLLNLFDFDRDRDGDRD"
BASE COUNT          685 a         1529 c         1762 g          697 t
ORIGIN      
        1 gccgtcggcg tccgggctgg cggatgccct ggcgggtgcc gtgaccgtgg cgggtggggc
       61 tgcaccgagc gctgggccga gcgctggact gcacgccggg acaagcaccc gatcgagcgc
      121 cggagcgaag gcgcagacgg cgagcgggac cggtgcggat gtgcagccga tgccgggtgc
      181 gctgcgctgg cggccgtggg cgctcggggc cgccgtcgtg ctggtgctgg tggcgatcgc
      241 ggctcgcgga tcgtccccgg tgagcgcgcc cggcgccgcg ttgccacgag atccgtccgg
      301 cgcgcagtcg ctggatcgcg aggaccgcga ggatcgcggc gagggcgacg atcgccgcga
      361 cctgcgcgac atccggatga cgccgcccgc ggggatggac gacaagcaaa cccgggactg
      421 gaacaagatc gccgacgcgc tccggcgcgg ccactacgag gacgcgcggc acaagctgcg
      481 cgagttcgag gacaagtacg gtcagaccga cgagacccgc gcgctcgcgc cgcagctcga
      541 tcagctcggg cccgacgatc cgcgcggtcc cggtggacca cccggccacg ggcgcggcaa
      601 gaagcatcac gactgatcga cggcagccct tgcgcgggct ccggtcaacc gggtccaact
      661 atccctgcgg cggagggcgg tcgtcggcgg cgcgcaggtc atccagtccg ccaccgttca
      721 cgacattcgt gtagccagca gcctccagac gggccttggc cctggcggcg cgcgctccgg
      781 cggcgcagta caccacgatc gggcgcgtgc gatcgccgcc gacgaggcgg tcgacctcgg
      841 ccaggcggcc ggggagctcg tcgaccggca ggttgaccgc gctcgccagg tggcgttcgg
      901 caaactcgtc cgcggtccgg acatcgatca cgacggcgcc cgacgcgatc agcgagcggg
      961 cggtcgccgg atccttgctc ggcggggcga ccaccgtgcg gtcggcctgg agctcgggct
     1021 cgtgcttcca gcaggtgcca aacgcgatcg cggcgaggaa cagcccgacc tggctcgcag
     1081 tgacgacgcc gatccgcacg gtctgtgtca tcgagatcaa catggcgagg tagcagtgca
     1141 cggtgcgttc cactccgagc agcgctgcaa gccggcgcaa cgggcccatc cagcgccgag
     1201 caacaccgca gtgggtgcac gccgagagcg cgcggcgcaa cggttgcgcg tgtcgagcgg
     1261 tctgcggcgc cgcgatccgc aactggcgaa tcgcgcgcgg ccgcgcgatc cacgccggcg
     1321 acatcgacgg caacgtcaag gtcgcgctgc tcggcgagac cgtggtcgag aagctatggg
     1381 ggccgaactt cgatccggtc ggccgcgcga tccggatccg cggcaacccg ttcgtcgtgg
     1441 tcggcgtgct ggagcgcaag ggccagtcgc cgatggggca ggactacgac gataccgcga
     1501 tcgtgccgta cacgacgttc cagaccaaga tctcgagcgg cgggctcggc gcgtacatcc
     1561 acgctgctcg cggcgatcgc ggtggtatcg ctggtggtcg gcgggatcgg catcatgaac
     1621 atcatgctgg tcagcgtcac cgagcgaacc cgcgagatcg gcgtgcggat ggcggtcggc
     1681 gcgcggtcct ggcacatcct cggccagttc ctcgccgagg cgctcgcgct gtcgctcgcc
     1741 ggtggcgcgc tcggcatcgc gctgggcctg acgatcgccg gccagctcgc ggcgcggttc
     1801 cacatgccct cgatgatcca ctggaacgtg atcgcgctcg ccgtcggatt ctcggcggcg
     1861 gtcggcgtcg gcttcgggct gtaccccgcg cgcaaggcgt cgcggctcga cccgatcgac
     1921 gcgctgcgct tcgagtgacc gtcggagcac tgcgcgcgcg tcgcggtgag cgcaggcagg
     1981 tggcggatca cctcggagtt tctcgctctc ctggcgggca gccccgcgga tcccagcaac
     2041 tctcgatcgg accagcgccc ggcgcgtccg ccttgtggaa caggaacggc atcatcgccg
     2101 tcgtgacgct gccgcgggcc gggaagtgcc aggcgcggac gtgctggcgc agacaaccac
     2161 cgagtctggt gagccaggac ggatcgacgc ccggcgcggt caccccgagc ctggcgacgc
     2221 tgccaccggg ctggatgatc agctccagcc gcagcgcacc cgcggcggcc ctgccgtggc
     2281 cgtagctggt atagcagctg cggacctcgg ggaggtaggg tgcgaaataa tactgtatct
     2341 ccttggcact gagcgtgtcg ggagcgtcgg cgtgtgcgac gccggacacg ccgagcatca
     2401 gccagacgag gaagcgcatg ctgattccag aagcagcatg cgcgccagat cggcgtccgg
     2461 cgctgttgcc acgcgcactg cgccgctcgc cgagccgcac tgcgctgcac gcggtgcgtc
     2521 ggcgagcagc gccctggcgc ggtcagtcgc gatcgccgtc gcggtcgcga tcgaagtcga
     2581 acaggttcag cagatcgccg atcgccgggc cgttgaccgg cacgtacgga ttggcgcgcg
     2641 acgcgaccgg gttgggcagg ttgtcgcggc tgcggcgcga caccggcggc aggttccagt
     2701 tgtgctcgat gaacttcagc agcgacacgt gatcgtaata cgtgtgatcg acgaatccct
     2761 gcttcgcgaa cggcgacacc acgatcatcg ggatccgggt gccgtcgccg aagaagtcga
     2821 ccgcctggat gtaacccgag tcgtagtagc cgccgccctc gtcggtggtg atgaagatcg
     2881 cggtggtcgc ccacagctcg ggatcggcct ggacctgtgc gatcaggtcg gcgaggaact
     2941 gctcgtagag gtccgcggtc gagtcggccg ggtggccggc cagggtctcg aacgggcgga
     3001 cgaacgacac cgcgggcagc gtgccggcgt ggacgtcgtg gaggaacgcg ccgtagctgg
     3061 cgagcttggc ctcctccgac ggattcttca tgatgctgga gaacccggtc agcggatcgc
     3121 agatcccgca gtaggcgtgg aaccgcagcg gcacgccgtc gaccgactgg gcgaacaccg
     3181 tcggatcatc accgcggtcg gccgagtagt acttccacgc cacgccgcgg cgggtcatga
     3241 cgtcggcgat cgtcggggcg ctctgcggcg gcagcgtgaa ctgatcggcg cccagcgtcc
     3301 gcgggttgct gcgggtctgg ttccagaaca gcgcgtagtt gttgagcagg tagaagtggt
     3361 cgcgctcgca gttggcgttg cgcacaccct ggcgatggag ctcgccgagc accgccgcga
     3421 cgccgggcgc gctggcgtcg gcgcagttga cgtacgaacc gccgctgtag ccgtcctgcg
     3481 tgtagtagtt gttggtgccg gcgaccggat cggggttctc gatctggttg gcgaacggcg
     3541 tcgccgcgct gccgtcgagg gtggtgctgg tgaagaacgc ggcgtggccg gtgaccaggg
     3601 ccatgaagtt ggcgccggtg ccgcccatga tcgcctggtg gtagttgtcg ctgatcgcga
     3661 agcggtcggc gagcgacttg aagaacggcg cgtcgccggg gctcgcgatg ccggcggcgt
     3721 tgacgaacgg gttcatgttg tagaacccca gcgagatcgc gccttccagg ggatcaaagc
     3781 cgccggcggg cggcggcgcg tcgttgccgc cgatgccgac ggtcttctcg acccacgcga
     3841 acttgtccat ccggccgccg tcgacgtcct gccacatctg gaagaagcgg tggaccggat
     3901 cgccggtgta ggccgcgtac ggcacgtact tgctgatctg gaacgggcca ttgggcaggt
     3961 tggccgggaa ccggacgtcg gggacgccgt cgggcaggcc gatgccgccc gaggtgaacg
     4021 gctggggcag gacgtcgtac gcgccggtcg acgcggtctc gacatggtag cggtcggtgt
     4081 cgctgccgat ccgctgctgg gcgcgggcga agtgcgggcc gggcgaaccg tcggcgttca
     4141 cgatcccctt ggacagcagg ttgtcgatgt gctggccgcg acggggctgg taggtgccga
     4201 acaggttgtc gaacgagtgg ttctcgccga ccacgatgat cgcgtgctgg atcggggtcc
     4261 gcgtcccggg gtgcgcgcgg cgcgccgtcg ctgcggcgtc tgcgccatcg ctcgccgggt
     4321 cggcgggagc ctgcacggcg cacgacgcgc tcgaagctgc gagtgcgagt cccgtggcga
     4381 tccagggcca cctgttgctc gatagtgtca tgatccctcc tgtacgaaat ggaagtgccg
     4441 gcggcggggc gcagccggcg gcgccaatct cgcgggtacg cgtgtcacgc cagacgtgat
     4501 cgcgtgtcgg ctcgatggcg gcgtgaccga tgccgatctc ccggcagatg agcggtgccg
     4561 cacgatacga ggcgcgtcgc tggcatggcg tgctgcaatt cgccgcgatg ccacccggcc
     4621 gtcggcgggg tgtcgcggtc gcgttaccgg ggcgtggtcg ggtacagacg tgg
//
LOCUS       DASVOR010002162         1547 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_81776,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002162 DASVOR010000000
VERSION     DASVOR010002162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81776"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF516_40120"
     CDS             <1..346
                     /locus_tag="VF516_40120"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEX8114027.1"
                     /translation="AAAAPAAAPARPADPQAPKIFAALARRLAENPALKQEVRATVQF
                     HVKDPDTSQTLELGGADPRKVDAVCTLGDADLAALASGKASARQLFQQGKLKIDGDIS
                     VGHRLGFLKGLI"
     gene            360..1541
                     /locus_tag="VF516_40125"
     CDS             360..1541
                     /locus_tag="VF516_40125"
                     /inference="COORDINATES: protein motif:HMM:NF006102.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-transfer protein"
                     /protein_id="HEX8114028.1"
                     /translation="MPRKVNVIGVGMVPFAKPGKSEEYHVMATKAGTAALADAKIPFD
                     QVQQAFAGYVYGDSTCGQRAIYQLGLTGIPVFNVNNNCSTGSSALMLGAQAVAGGVAE
                     CVIVVGFEQMEAGALSAKWTDRANPLDKFVQVMNEEQGFSQAPPAAQMFGGAGREYRW
                     KHGTKRETFAKISAKARQHAAKNPYALFKDLLSVEEILASPEVFDPLTRYQCCPPTCG
                     AAAAVLASDEFVKKHGIDRPVYIAAQTMTTDYPSSFEEHSMIKMVGYDMAKSAAAKVY
                     QQTGIGPDDVDVIELHDCFTANELLTYEALGLCKEGEAEKFIWDGDNTYGGKFVTNPS
                     GGLLSKGHPLGATGLAQCTELVWQLRGQAEARQVEGARIALQHNLGLGGACVVTMYRR
                     D"
BASE COUNT          273 a          550 c          523 g          201 t
ORIGIN      
        1 ggcggccgcg gcgcccgcgg cggcgccggc caggcccgcc gacccgcagg cgccgaagat
       61 cttcgccgcg ctggccaggc ggctcgccga gaacccggcg ctcaagcagg aggtccgcgc
      121 gacggtgcag ttccacgtca aggaccccga cacgtcccag acgctcgagc tcggcggcgc
      181 cgaccccagg aaggtcgacg cggtgtgcac gctgggcgac gccgacctcg cggcgctggc
      241 gagcggcaag gccagcgcgc gccagctgtt ccagcaaggc aagctcaaga tcgacggcga
      301 tatctccgtc ggtcatcgcc tcggattcct caaaggcctg atctaaggag acccatcaca
      361 tgccacgcaa agtcaacgtc atcggcgtcg ggatggtccc gttcgccaag cccggcaaga
      421 gcgaggagta tcacgtgatg gcgaccaagg ccggcacggc cgcgctcgcc gacgcgaaga
      481 tcccgttcga tcaggtccag caggcgttcg ccggctacgt ctacggcgac tcgacctgcg
      541 gccagcgcgc gatctaccag ctcggcctga ccggcatccc ggtgttcaac gtcaacaaca
      601 actgctcgac cggcagctcg gcgctgatgc tcggcgccca ggcggtcgcc ggcggggtcg
      661 ccgagtgcgt gatcgtcgtc gggttcgagc agatggaggc cggcgcgctg tcggcgaagt
      721 ggaccgatcg cgccaacccg ctcgacaagt tcgtccaggt gatgaacgag gagcagggct
      781 tcagccaggc gccgccggct gcccagatgt tcggcggcgc cggccgcgag taccgctgga
      841 agcacggcac caagcgcgag acgttcgcca agatcagcgc gaaggcccgc cagcacgcgg
      901 ccaagaaccc gtacgcgctg ttcaaggacc tgctgtcggt cgaggagatc ctggcgtcgc
      961 ccgaggtgtt cgatccgctg acccgctacc agtgctgccc gccgacctgc ggcgccgccg
     1021 ccgcggtgct cgccagcgac gagttcgtca agaagcacgg catcgaccgc ccggtctaca
     1081 tcgccgccca gaccatgacc accgactacc cgtcgagctt cgaggagcac tcgatgatca
     1141 agatggtcgg ctacgacatg gcgaagtccg ccgcggccaa ggtctaccag cagaccggga
     1201 tcgggcccga cgacgtcgac gtgatcgagc tccacgactg cttcaccgcc aacgagctgt
     1261 tgacctacga ggcgctcggc ctgtgcaagg aaggcgaggc cgagaagttc atctgggacg
     1321 gcgacaacac ctacggcggc aagttcgtca ccaacccgtc gggcggcctc ttgtccaagg
     1381 gccacccgct cggcgcgacc gggctcgccc agtgcaccga gctggtctgg cagctgcgcg
     1441 gccaggccga ggcccgccag gtcgagggcg cgcggatcgc gctgcagcac aacctcgggc
     1501 tcggcggcgc ctgcgtggtc acgatgtacc gccgcgactg aacgcga
//
LOCUS       DASVOR010002163         2878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_490947,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002163 DASVOR010000000
VERSION     DASVOR010002163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490947"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VF516_40130"
     CDS             complement(<1..199)
                     /locus_tag="VF516_40130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114029.1"
                     /translation="MKHASNAIVAAVLGTAAIARAQPAPGDPPKPDGQAGAPRGGPAP
                     SPQSPYGPDPGPPAPAGQVVTR"
     gene            complement(225..1166)
                     /locus_tag="VF516_40135"
     CDS             complement(225..1166)
                     /locus_tag="VF516_40135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114030.1"
                     /translation="MQRALVGSVAPVLAAMLAGNAASDPAPQSADVVGEVPRTPDTTI
                     GTMFRGPFRSARLFAMPTADVVGAYILTLSGEGSLLQKPGVLTSEGVLAVGFGDIAQL
                     EYRHTEAIGITGLNAPVPAVGVQLKLPIPERPNVPAFGVAFRLGVPRGELLDGRNVDE
                     TVTDLYLVGRLRFAFAPWLTLHGGTRISFAKAVPADPNLLAQRTLWLPTAGYEVAMNP
                     WSTIVGEIALAPQFTWMPVVQPSPMIGRGVLGRLGMKWRILPSIALEGSIGYQIDNSV
                     SPEGVEAIVTAWDIRLGAAVFVPWGALACRAVGVFCN"
     gene            1376..>2878
                     /locus_tag="VF516_40140"
     CDS             1376..>2878
                     /locus_tag="VF516_40140"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114031.1"
                     /translation="MNRLPRPSRSRRLLAQSPPALWRTGPSDDEGRGYLQARMTLYSR
                     LMFWSIVALLVFLTAMYEIYPAIKPRHNDVIFIGATIALGVMAILWQGVLVRRVVSVR
                     TLYALDVVYASGIGGSFATGALLASDLRPAGYTTLIYAAFTVFMRAIVVPSSGRRTAV
                     VSLLVFVPMWTTAAILAVETTQEVPAPAFVVGALVLGVVPILLASVGSNIIYGLRRQV
                     SAAMQLGQYTLDSKIGEGGNGAVYRAHHALLRRPTAIKLLLPDRVGADNLDRFEREVQ
                     HMSQLTHPNTVAVFDYGRSPDGVFYYAMEYLGGIDLEHLVERFGPQPSGRVVHILVQV
                     CGALHEAHAARLIHRDIKPANIILCERGAVLDVAKVVDFGLVKEITRDTGASTQVVLG
                     TPAYVAPEAVTDPDHITPAVDLYALGAVGYFLITGRPVFEGKTAVDVCIQHVTQPPQP
                     PSELGIAIAADLEAVLMRCLAKQPAQRYPSAAALAEALEALPPAGDWRKAD"
BASE COUNT          396 a         1060 c          998 g          424 t
ORIGIN      
        1 gtcgcgtcac cacctgaccg gccggtgcgg gcgggccggg atccggaccg tacgggctct
       61 gcggactcgg cgccgggccg ccgcgcggag cgcctgcctg gccgtcgggt tttggcggat
      121 cgccgggcgc cggctgtgcc cgggcgatcg ccgcggtgcc gagcaccgcc gcgacgattg
      181 cgttgcttgc atgcttcatc acgtcatctc cgcacgcccg cgcgctagtt gcagaacacc
      241 ccgacggcgc ggcaggccag cgcgccccag ggcacgaaca ccgcggcacc gagccggatg
      301 tcccaggcag tcacgatcgc ctcgacccct tcgggcgaga cggagttgtc gatctggtag
      361 ccgatgctgc cctcgagggc gatcgacggc aggatccgcc acttcatccc gagccggccg
      421 agcacgccgc ggccgatcat cggcgacggc tggaccaccg gcatccaggt gaactgcggc
      481 gccagcgcga tctcgccgac gatcgtcgac cacgggttca tcgcgacctc gtagccggcg
      541 gtcggcagcc acagcgtccg ctgcgcgagc aggttcggat cggcgggcac cgccttggcg
      601 aacgagatcc gcgtgccgcc gtgcagggtc agccacggcg cgaacgcgaa ccggagccgg
      661 ccgaccaggt agaggtcggt gacggtctcg tcgacgttcc ggccgtcgag cagctcgccg
      721 cgcggcacgc cgagccggaa cgcgacgccg aacgccggga cgttgggccg ctcggggatc
      781 ggcagcttga gctgcacgcc gaccgccggc accggcgcgt tgagcccggt gatcccgatc
      841 gcctcggtgt ggcggtactc gagctgcgcg atgtcgccga acccgaccgc gagcacaccc
      901 tccgaggtca gcacgcccgg cttctgcagc aggctgccct cgcccgacag cgtcaggatg
      961 tacgcgccga ccacgtcggc ggtcggcatc gcgaacagcc gcgcgctgcg gaacggtccg
     1021 cggaacatcg tgccgatcgt ggtgtccggc gtgcgcggca cctcgccgac gacatcggcg
     1081 ctctgcggcg ccggatcact cgcggcgttg cccgcgagca tcgctgcgag caccggcgcg
     1141 accgacccga cgagtgcgcg ttgcatgcgg acagttgtga ggtcggacca cgtgaattcg
     1201 ccagcgcgtc gccgtgatct gcgcgtgatc gccacacgct gccgcgtgtc aggatccggt
     1261 caccgatgcc tgtcaagatc cggacatccg ccgcgattcc ccgacgacgg tcgccgtgcc
     1321 gattggcgtg aaatccacgt gatccgcgcg ccgtgctgac ggatgctgtg ctggcgtgaa
     1381 tcgcttgccc aggccatcgc ggtctcgccg gttgctggcg caatcgccgc ccgcgctgtg
     1441 gcgcaccggt ccctccgacg acgaaggccg cggctacctg caggcccgga tgacgctgta
     1501 ctcgcggctg atgttctgga gcatcgtcgc gctgctcgtg ttcctgaccg cgatgtacga
     1561 gatctatccc gcgatcaagc cgcggcacaa cgacgtcatc ttcatcggcg cgacgatcgc
     1621 gctcggcgtg atggcgatcc tgtggcaggg ggtcctggtc cgtcgcgtgg tctcggtgcg
     1681 cacgctgtac gcgctcgacg tggtctacgc gagcggcatc ggcggctcgt ttgcgaccgg
     1741 cgcgctgctc gccagcgacc tgcgcccggc cggctacacc acgctgatct acgccgcgtt
     1801 caccgtgttc atgcgcgcga tcgtcgtgcc cagcagcggc cggcgcaccg cggtggtgtc
     1861 gctgctggtg ttcgtgccga tgtggaccac cgccgcgatc ctcgcggtcg agaccaccca
     1921 ggaggtgccg gccccggcgt tcgtggtcgg cgcgctcgtg ctcggcgtcg tgccgatcct
     1981 gctcgcctcg gtcggctcca acatcatcta cggcctgcgc cgccaggtgt ccgccgcgat
     2041 gcagctcggc cagtacacgc tcgacagcaa gatcggcgag ggcggcaacg gcgcggtgta
     2101 ccgcgcgcac cacgccctgc tccgccggcc gaccgcgatc aagctgctgt tgcccgaccg
     2161 ggtcggcgcc gacaacctcg atcgcttcga gcgcgaggtc cagcacatga gccagctgac
     2221 ccaccccaac accgtcgcgg tgttcgacta cgggcgcagc cccgacggcg tgttctacta
     2281 cgcgatggag tacctgggcg gcatcgacct cgagcacctg gtcgagcggt tcgggccgca
     2341 gccgtcgggc cgggtcgtcc acatcctggt ccaggtgtgc ggcgcgctcc acgaggccca
     2401 cgccgcccgg ctgatccacc gcgacatcaa gccggccaac atcatcctgt gcgagcgcgg
     2461 cgcggtgctc gacgtcgcca aggtcgtcga cttcggcctg gtcaaggaga tcacgcgcga
     2521 caccggcgcg tcgacccagg tcgtgctcgg caccccggcc tacgtcgcgc ccgaggcggt
     2581 gaccgacccc gaccacatca cgcccgccgt cgatctctac gcgctgggcg cggtcggcta
     2641 cttcctgatc accgggcgcc cggtgttcga gggcaagacc gcggtggatg tctgcatcca
     2701 gcacgtgacc cagccgccgc agccgccgtc cgagctcggg atcgcgatcg ccgcggacct
     2761 cgaggcggtg ctgatgaggt gcctggccaa gcagccggcg cagcgctacc cgtcggccgc
     2821 ggcgctcgcc gaggcgctcg aggcgctgcc gccggcgggc gactggcgca aggccgac
//
LOCUS       DASVOR010002164         2105 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_344059,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002164 DASVOR010000000
VERSION     DASVOR010002164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344059"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2105
                     /locus_tag="VF516_40145"
     CDS             <1..>2105
                     /locus_tag="VF516_40145"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8114032.1"
                     /translation="VDGVTLVPGAPSASFAARRDGVALVTAADVFGQRTHHAQRQRKR
                     AKDALLGGVGDFSMLSPGDYLVHQRHGVGRYHGLEKIAVGAASLLQTQAIGAAAPALQ
                     QIDALKLEYDGGTLYLPVYRLGEVQRYVGAEGHTPRLDKLGGQTWEATRSKVARHVRA
                     LAEELLQLYAQRAALPGHRFPPADDGFRELEATFPFDETPDQAAAIDAVLTDMESPRA
                     MDRLVCGDVGYGKTEVALRAIFRAVQGGKQACLLAPTTVLVEQHFRTMTERFAGFPVN
                     IGKLSRFQPRGEQIETVKKAAEGKLDVVVGTHRLLSPDVRWKDLGLLVIDEEQRFGVA
                     HKERIKKSRTQVDVLTLTATPIPRTLHLAMANLRDLSIIATPPADRRAVRTFVSRIDD
                     TTIREAIERELGRGGQVFFIAPTIGNMGLRARDPRLPNPDEPPRRTRPRDDDRTIDEW
                     TEYLQGLVPAARIGIGHGALPADQLEDVMLRFVRGELDVLVATTIVESGLDIPRANTM
                     FIARADAFGLAQLYQLRGRIGRSKDRAYCYLLVPEPEHITDEARRRLETLQRFTELGA
                     GFQIASADLEIRGGGELLGAKQSGSIAAVGFDQYVKMLEAVVAELGGKPIHSAIDPEL
                     AIETPGFIPDDYVPDPGQRLELYKRLSAVETDDELQGVMTEIADRYGPIPGDVALLGE
                     LMGVKAIARGAGALALEIS"
BASE COUNT          308 a          780 c          755 g          262 t
ORIGIN      
        1 cggtcgacgg ggtgacgctg gtccccggcg cgccgagcgc aagcttcgcg gcgcgccgcg
       61 acggggtcgc gctggtcacc gcggcggacg tgttcggcca gcgcacccac cacgcccagc
      121 gccagcgcaa gcgcgccaag gacgcgctgc tcggcggagt cggcgacttc tcgatgctgt
      181 cgcccggcga ctacctggtc caccagcgcc acggcgtcgg ccgctaccac ggcctggaga
      241 agatcgcggt cggcgcggcg tcgctgctcc agacccaggc gatcggcgcg gcggcgcccg
      301 cgctgcagca gatcgacgcg ctcaagctcg agtacgacgg cggcacgctg tacctgccgg
      361 tgtaccggct cggcgaggtc cagcgctacg tcggcgccga gggccacacg ccccggctcg
      421 acaagctcgg cggccagacc tgggaggcga cgcgcagcaa ggtcgcgcgc cacgtccggg
      481 cgctcgccga ggagctgctc cagctctacg cccagcgcgc cgcgctgccg ggccaccggt
      541 tcccgcccgc cgacgacggc ttccgcgagc tcgaggcgac gttcccgttc gacgagaccc
      601 cggatcaggc cgcggcgatc gacgcggtgc tcaccgacat ggagtcgccg cgcgcgatgg
      661 accgcctggt gtgcggcgac gtcggctacg gcaagaccga ggtcgcgctg cgcgcgatct
      721 tccgcgcggt gcagggcggc aagcaggcct gcctgctggc gccgaccacc gtgctggtcg
      781 agcagcactt ccggaccatg accgagcggt tcgccgggtt cccggtcaac atcggcaagc
      841 tgtcgcggtt ccagccccgg ggcgagcaga tcgagaccgt gaagaaggcc gccgagggca
      901 agctcgacgt cgtggtcggg acccaccgcc tgctgtcgcc cgacgtgcga tggaaggacc
      961 tcggcctctt ggtgatcgac gaggagcagc ggttcggcgt cgcccacaag gagcggatca
     1021 agaagtcgcg gacccaggtc gacgtgctga ccctgaccgc gacgccgatc ccgcgcaccc
     1081 tgcacctcgc gatggccaac ctgcgcgacc tgtcgatcat cgcgacgccg cccgccgacc
     1141 gccgcgcggt gcgcacgttc gtgtcgcgga tcgacgacac cacgatccgc gaggcgatcg
     1201 agcgcgagct cggccgcggc ggccaggtgt tcttcatcgc cccgacgatc ggcaacatgg
     1261 gcctgcgcgc gcgcgacccc cggctgccca accccgacga gccgccgcgc aggacccggc
     1321 cgcgcgacga cgatcgcacg atcgacgagt ggaccgagta cctccagggg ctggtcccgg
     1381 cggcgcggat cgggatcggc cacggcgcgc tgccggccga ccagctcgag gacgtgatgc
     1441 tccggttcgt gcgcggcgag ctcgacgtgc tggtcgcgac cacgatcgtc gagagcgggc
     1501 tcgacatccc gcgcgccaac acgatgttca tcgcgcgcgc cgacgcgttc ggcctggccc
     1561 agctctacca gctgcgcggc cggatcgggc gcagcaagga ccgcgcgtac tgctacctgc
     1621 tggtgcccga gcccgagcac atcaccgacg aggcgcgccg ccggctcgag accctgcagc
     1681 ggttcaccga gctcggcgcg gggttccaga tcgcgtcggc cgacctcgag atccgcggcg
     1741 gcggcgagct gctcggcgcc aagcagtcgg gctcgatcgc cgcggtcggg ttcgatcagt
     1801 acgtcaagat gctcgaggcg gtggtcgccg agctcggcgg caagccgatc cactccgcga
     1861 tcgaccccga gctcgcgatc gagaccccgg ggttcatccc cgacgactac gttccggatc
     1921 cgggccagcg gctcgagctc tacaagcggc tgtccgcggt cgagaccgac gacgagctcc
     1981 agggcgtgat gaccgagatc gcggaccgct acggcccgat cccgggcgac gtcgcgctgc
     2041 tcggcgagct gatgggggtc aaggcgatcg cgcgcggcgc cggcgcgctg gcgctcgaga
     2101 tcagc
//
LOCUS       DASVOR010002165         6756 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_549793,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002165 DASVOR010000000
VERSION     DASVOR010002165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6756
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549793"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..633)
                     /locus_tag="VF516_40150"
     CDS             complement(<1..633)
                     /locus_tag="VF516_40150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114033.1"
                     /translation="MEFRRQLVVGHSALLLVTLITAAVAAIALRISSARLESVSRDLV
                     ADMFAIQRLQLRAEQLVSTSRGYLLTGEARSLGRFDDAARAFGEALAEIDRRRRALGD
                     EVAALARAANGYVTLARHAARERAATGDPKDILPFFEQDLAPARDHFEVALSQFAGRE
                     QAAFDRASRGAHRLASRAQVTVLVTTALSIALAVALAVTSSRKLAAQYARS"
     gene            985..1260
                     /locus_tag="VF516_40155"
     CDS             985..1260
                     /locus_tag="VF516_40155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114034.1"
                     /translation="MPGCPDRRSPRWWRRSPRSRSSLRCCDFRAVEVAQQIALAHGGD
                     IDVASSSAAGTTVTIRLPRNAEAAEATAESTSRSHSHGAVSGPRAPA"
     gene            1636..2817
                     /locus_tag="VF516_40160"
     CDS             1636..2817
                     /locus_tag="VF516_40160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114035.1"
                     /translation="MIERAFDKIPNNLPIPDSAGFAASFHPSGSIDLSNSFFTPQGSN
                     GRHCGTCHAPEDGWSITPATVALMFVLTGGNDPLFVNHLDTDTPTSDMSTPAARWRST
                     TMLRQGKFTRKVSPPAVRDYEVIAASDPFGVGTTSSLWFFRRPLPAANFRSATVMWDG
                     ANTVATGLRDGLIKQARSNVTGAQQGAPAADAVIFDIVDYERALSYAQLIIPSVGRLD
                     ADGAHGGPEAANAQPLVEGRFDLYDAWQHSGNPRRRQIWRGQEIFNNVNPPSGHRCGG
                     CHTAANNGQNVNGLLFDVGLSRPELARPDMAVYTFRRISDGAIVQTTDPGRGIRTGNF
                     ADLNHFKVPSLRGLAARAPYFHNGLAASLEDVVALYQRSLGFVFTPEEAADLVAFLQA
                     L"
     gene            complement(2840..3628)
                     /locus_tag="VF516_40165"
     CDS             complement(2840..3628)
                     /locus_tag="VF516_40165"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114036.1"
                     /translation="MDTKNLFSLQGKTALITGGSRGIGKMIADGFLAQGARVYLSSRK
                     ADACQETAAELSRGGGTCVALPMDVSTVEGARGLAAAYAAHESSLDILVNNAGAAWLA
                     GFDDFPEKGWDKVMDLNAKSPFFVTQALAAPLRAAARPDRPAKVINIASIDGIYLTPL
                     ETYSYAASKSALIQLTRLMACRLARDHIVVTAIAPGPFKSDMNIRARDHEAEVAAQVP
                     VQRIGTDLDMAGAAIFLASRAGDFVVGSVITVDGGIAFATSPAR"
     gene            complement(3681..4019)
                     /locus_tag="VF516_40170"
     CDS             complement(3681..4019)
                     /locus_tag="VF516_40170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114037.1"
                     /translation="MTPAKRILGVAGLSTLATLALAAGMATRAPARPVTYTLPSESST
                     LKKTGEPGYQRAELQCVTCHSRDYITTQPRGKGKEFWTAEVTKMVNVYAAPIPEPDRA
                     VIADYLAATY"
     gene            complement(4016..5224)
                     /locus_tag="VF516_40175"
     CDS             complement(4016..5224)
                     /locus_tag="VF516_40175"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8114038.1"
                     /translation="MLDRRHFITGAGGLAAALAAGRLLGRRAWAQPAALPFANGERPL
                     VQYPQKRPMIRLTERPPQLETPFSVFDEGPITPNDAFFVRYHLASIPQQIDPDAFRLE
                     VDGKVGKPLSLSLAELKAMPAIEVVAVNQCSGNSRGFFEPRVAGGQLANGAMGNARWK
                     GVPLKAVLDKAGVQAGARQVVLDGLDKPVIAATPDFVKSLEIDHARDGEVLLAWAMNG
                     ADLPWLNGFPLRVVVPGYYGTYWMKHVNQITVIDGELDSFWMKTAYRIPDNECACVPA
                     GSKPDKTRPIGRLDVRSFITNLADQARVKAGAATAVRGIAFDGGHGIKEVALSIDGGK
                     TWKPTQLGRDLGKYSFRAWQTTVKLPAGAQALMVRATSKTGEVQPMEPRWNPSGYMRN
                     IVETVHVEAA"
     gene            complement(5325..5996)
                     /locus_tag="VF516_40180"
     CDS             complement(5325..5996)
                     /locus_tag="VF516_40180"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX8114039.1"
                     /translation="MTSDTKTDEQIQRDVLAELQWDSRVQPSEIGVSVESGIVALRGH
                     VDSYYKKWAAEEAALRVRGVVAVASELEIKLPSSSERTDEEVAAAAVRALESDALLGA
                     KNLQITVDKCSITLRGEVDWKFQKEDAERILRRLWGVKGVTNLITVKARPTPMELEDQ
                     IEKALLRTAQLDAQQISVDVQGSKVTLKGTVRSWAEREESERAAWLAPGVTQVDNRIQ
                     VAYAA"
     gene            6274..>6756
                     /locus_tag="VF516_40185"
     CDS             6274..>6756
                     /locus_tag="VF516_40185"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEX8114040.1"
                     /translation="MTRVRTWRWWTIALRGLVAIAFGILAMIRPGAAFLSLVLLFGVY
                     AIVDGVLALVLASQRVHARGALVARGIVSIAAGVLALEWPGITAFVLLLVIAGWAIVS
                     GVLEIAMAIQMRKQLESEWLLALEGALSVVFGVLLLLSPLVGAIVLGLWIGAWALVLG
                     G"
BASE COUNT          950 a         2374 c         2377 g         1055 t
ORIGIN      
        1 cgatcgcgcg tactgcgcgg cgagcttccg gctcgacgtc acggcgagcg cgacggcgag
       61 cgcgatcgac agcgcggtcg tcacgagcac ggtgacctgg gcgcgcgacg cgagtcggtg
      121 ggcaccgcgc gacgcgcggt cgaacgccgc ctgctcgcgg ccggcgaact ggctcagcgc
      181 gacctcgaag tggtcgcgcg ccggggccag gtcctgctcg aagaacggca ggatgtcctt
      241 cggatccccg gtcgccgcgc gctcgcgcgc ggcgtggcgc gccagcgtga cgtagccgtt
      301 ggccgcgcgg gcgagcgccg cgacctcgtc gccgagagcg cggcgccggc gatcgatctc
      361 cgccagcgcc tcgccgaacg cgcgcgcggc gtcgtcgaag cggccgagcg acctcgcctc
      421 gccggtcagc aggtagccgc ggctggttga gaccagctgc tcggcccgga gctgcagccg
      481 ctggatcgcg aacatgtcgg cgaccagatc gcgcgacacg gactccaggc gcgcgctgga
      541 gatccgcagc gcgatcgcgg cgaccgcggc ggtgatcagc gtgacgagca acagcgcgga
      601 atgtcctaca acgagctgcc ggcgaaattc catgagctca cgtcgggccc cacgagctgt
      661 gaaccgcccg cccgcgccag gtgttccatg atccggcagg acccggcaag gccagcggcg
      721 gacggcgcac accgctcgtc ccgtccatga gccagccaac catcgcgttc ctcggcgaac
      781 cggtccggcg ttcccggatc cgggccccga gcgcgagcgc acactggtcg cggacgccac
      841 atcctccgaa gtcgccgaga tccctgcccg gcccgcgaga cgcggtcgcg gcgttgtgcc
      901 atgtctcggg agagcacagg gtcttggtcc cccaggcgta cgagcacgtt gtggatcgca
      961 caacatcgcc cgctcccagg tcgcgtgcct ggctgccccg accggcgctc gccgcgctgg
     1021 tggcggcgct cgccgcggtc gcggtcatcg ctgcgctgtt gcgacttccg cgcggtcgag
     1081 gtcgcgcagc agatcgcgct cgcccacggc ggcgacatcg acgtcgcgtc gagctccgcc
     1141 gccggcacca ccgtcacgat ccggctgccg cgcaacgccg aggcggccga ggcgaccgcc
     1201 gagagtacga gccggtcgca cagccacggt gcggtcagcg ggccgcgcgc gccggcctag
     1261 actcccgcac gcatcgcatc gcatcccacc gcacccccgt acgagccgtt cgtacattcc
     1321 ccggtcgtgt cgcggaatac cgcgtattgc cgccgatagc ccgccagcgt cgcactgcga
     1381 ccgttccggt acagcgcgat ccgtaccgga acaccgtagt ttctcgacga cggtcggcgg
     1441 ctacatgaga tcaattctca ctttgccaac aaaatagggt gcatttcgct gcatgaacat
     1501 tctagggttc agtgagcgga ggctaaccac gtgagcgttc acaattacac tgcattgtcc
     1561 acgttgttct ctgcagttct cctcggcgca ggatgcgcgc aagcaaccca ggatgtgagt
     1621 gcagataggc aaggattgat cgagcgagcg ttcgacaaga tcccgaacaa cctaccgatt
     1681 cccgacagcg cgggattcgc cgcgtcgttt catccatctg gctccatcga tctgagcaac
     1741 agcttcttca caccgcaggg cagcaacggg cgccactgtg ggacctgtca cgctcccgag
     1801 gacggctgga gcatcacgcc ggcgaccgtc gcgctgatgt tcgtgctgac cggcggcaac
     1861 gacccgctgt tcgtcaacca cctcgatacc gacacaccga cctccgacat gtcgactccg
     1921 gcggcgcgct ggaggtcgac cacgatgctg cggcagggca agttcacccg caaggtatcg
     1981 ccgccggcgg tccgcgacta cgaggtgatc gcggccagcg atccgttcgg cgtcggcacc
     2041 acctcgagcc tgtggttctt ccgccggccg ttgcccgccg cgaacttccg cagcgccacc
     2101 gtgatgtggg acggcgccaa cacggtcgcc accggactgc gcgacggcct gatcaagcag
     2161 gcgcgcagca acgtgaccgg cgcccagcag ggcgcgcccg ccgccgacgc cgtgatcttc
     2221 gacatcgtcg actacgagcg cgcgctgtcg tacgcgcagc tgatcattcc ctcggtggga
     2281 cggctcgacg ccgacggcgc gcacggcggc ccggaggcgg ccaacgccca gccgctggtg
     2341 gaaggccggt tcgacctcta cgacgcctgg cagcacagcg gcaacccgcg gcgccgccag
     2401 atctggcgcg gccaggagat cttcaacaac gtcaacccgc cgagtggcca ccgctgcggc
     2461 ggctgccaca ccgccgccaa caacggccag aacgtcaacg gcctgctgtt cgatgtcggc
     2521 ctgtcgcggc ccgagctcgc ccggcccgac atggcggtct acacgttccg gcggatcagc
     2581 gacggcgcga tcgtgcagac caccgatccg ggccgcggca tccgcaccgg caacttcgcc
     2641 gatctgaacc acttcaaggt ccccagcctg cgcggcctgg ccgcgcgcgc gccgtacttc
     2701 cacaacgggc tcgcggcctc gctggaagac gtcgtcgccc tctaccagag gtcgctcggc
     2761 ttcgtgttca cgcccgagga ggccgccgac ctggtcgcgt tcctccaggc gctgtgatcg
     2821 cggcgccgcg gatcgccgat cagcgcgccg gcgaggtcgc gaacgcgatg ccgccgtcga
     2881 ccgtgatcac cgagccgacc acgaagtcgc cggcgcgcga ggcgaggaag atcgccgcgc
     2941 ccgccatgtc gaggtcggtg ccgatccgct ggaccgggac ctgggccgcg acctcggcct
     3001 cgtggtcgcg cgcgcggatg ttcatgtcgg acttgaacgg gcccggcgcg atcgcggtga
     3061 cgacgatgtg gtccctggcg agccggcacg ccatcagccg ggtcagctgg atcagcgccg
     3121 acttgctggc ggcgtacgag taggtctcga gcggcgtcag gtagatgccg tcgatcgagg
     3181 cgatgttgat caccttggcc gggcgatcgg ggcgcgccgc cgcgcgcagc ggggccgcga
     3241 gcgcctgggt cacgaagaac ggcgacttgg cgttgaggtc catcaccttg tcccagccct
     3301 tctcgggaaa gtcgtcgaac ccggcgagcc aggcggcgcc ggcgttgttg accaggatgt
     3361 cgagcgagct ctcgtgcgcg gcgtaggccg cggccagccc gcgcgcgccc tcgacggtcg
     3421 acacgtccat cggcagcgcg acgcaggtgc cgccgccgcg cgacagctcc gcggcggtct
     3481 cctggcatgc gtcggccttg cgcgacgaga ggtagacccg ggcgccctgg gcgaggaagc
     3541 cgtcggcgat catcttgccg atgccgcgcg agccgccggt gatcagcgcg gtcttgccct
     3601 ggagcgagaa caggttcttg gtgtccatcg gtcgcctcgt ggttggctcg gggttgcctg
     3661 gcgggtgggt cagccggggt tcaataagtc gccgcgagat agtcggcgat caccgcgcga
     3721 tcgggctcgg ggatcggagc ggcatagaca ttgaccatct tggtgacctc ggcggtccag
     3781 aactccttgc ccttgccgcg cggctgggtg gtgatgtagt cgcgcgagtg gcaggtcacg
     3841 cactggagct ccgcccgctg gtagcccggc tcgccggtct tcttcagcgt cgagctctcg
     3901 gacggcaggg tgtaggtgac cggcctggcc ggtgcgcggg tggccatccc ggcggcgagc
     3961 gccagggtcg ccagggtcga gagcccggcc acgccgagga ttcgcttcgc gggcgtcatg
     4021 cggcctccac gtggacggtc tcgacgatgt tgcgcatgta gcccgacggg ttccaccgcg
     4081 gctccatggg ctggacctcg ccggtcttgc tggtcgcgcg gaccatcagg gcctgggcgc
     4141 cggccggcag cttgaccgtg gtctgccagg cgcggaacga gtacttgccg aggtcgcggc
     4201 cgagctgggt cggcttccag gtcttgccgc cgtcgatcga cagcgcgacc tccttgatgc
     4261 catggccgcc gtcgaacgcg atgccgcgca ccgcggtggc ggcgccggcc ttgaccctgg
     4321 cctggtcggc gaggttggtg atgaacgacc ggacgtcgag ccggccgatc ggccgggtct
     4381 tgtcgggctt gctgccggcc ggcacacagg cgcattcgtt atccggaatg cgataggcgg
     4441 tcttcatcca gaagctgtcg agctcgccgt cgatcacggt gatctggttg acgtgcttca
     4501 tccagtacgt gccgtagtag ccgggcacca cgacgcgcag ggggaaaccg ttgagccacg
     4561 gcaggtcggc gccgttcatc gcccacgcca gcaggacctc gccgtcgcgc gcgtggtcga
     4621 tctcgagcga cttgacgaag tcgggcgtcg cggcgatcac cggcttgtcg aggccgtcga
     4681 gcacgacctg gcgcgccccg gcctggacgc cggccttgtc gagcacggcc ttgagcggca
     4741 cgcccttcca gcgcgcgttg cccatcgcgc cgttggcgag ctggccgccg gcgacgcgcg
     4801 gctcgaagaa cccgcggctg ttgcccgagc actggttgac cgcgacgacc tcgatcgccg
     4861 gcatcgcctt gagctcggcg agcgacagcg acagcggctt gccgaccttg ccgtcgacct
     4921 cgagccggaa cgcgtccgga tcgatctgct gcgggatgct ggcgaggtgg tagcggacga
     4981 agaacgcgtc gttgggcgtg atcggcccct cgtcgaacac gctgaacggc gtctcgagct
     5041 gcggcggccg ctcggtgagc cggatcatcg gccgcttctg cgggtactgg accagcggcc
     5101 gctcgccgtt tgcgaacggc agggccgccg gctgggccca ggcccgccgg ccgagcagcc
     5161 tgccggcggc gagcgcggcc gcgaggcctc cggcaccggt gatgaagtga cgcctgtcga
     5221 gcatcgatcc ctccgctggt gcgcaccagc cacgtttccg caggaacttc tctggttttt
     5281 gcggacgggt caacaggcac agcgccggca tgtgccgcgg cgggtcaggc ggcgtacgcc
     5341 acctggatgc ggttgtcgac ctgggtgacg ccgggcgcca gccatgcggc gcgctcggac
     5401 tcctcgcgct cggcccacga ccgcacggtg cccttcaggg tgaccttgga gccctggaca
     5461 tcgacggaga tctgctgggc gtcgagctgc gcggtgcgga gcaacgcctt ctcgatctgg
     5521 tcctcgagct ccatcggcgt cggccgcgcc ttgaccgtga tcaggttggt cacgcccttg
     5581 accccccaca gccggcgcag gatgcgctcg gcgtcctcct tctggaactt ccagtcgacc
     5641 tcgccgcgca gggtgatcga gcacttgtcg acggtgatct gcaggttctt cgcgccgagc
     5701 agggcatccg attccagcgc gcgtacggcg gccgcggcga cctcctcgtc ggtgcgctcc
     5761 gacgacgatg gcagcttgat ctcgagctcg ctggcgaccg cgaccacgcc gcggacgcgg
     5821 agcgcggcct cctcggctgc ccatttcttg tagtacgaat cgacgtgtcc gcgcagcgcc
     5881 acgatcccgc tctccacgct gaccccgatc tcgctgggct ggacgcgaga atcccactgc
     5941 agctcggcga ggacgtcgcg ctggatctgt tcgtcggtct tggtatcgct cgtcatgaat
     6001 cctcccgacg gccgcggatg caaggccggt gcgcacggcc ggtggcacgc gtccgcgact
     6061 tgcccgtggg ccccccggca agcacgcgcc acggcgcgcg tcgcgcggtg tcgacccgct
     6121 cgagggccgc gggaccgggc agctcgcgtc acgggccgtg gtgtgcacga tgcatgtgga
     6181 tcgttgcgag ccattccagc tccggaggaa gcgcatgacg agcccacgtc cccagcaccg
     6241 gtcgcacaga ccgcgtcgca tcaggaggat ccgatgaccc gagtcaggac atggcggtgg
     6301 tggaccatcg cgctgcgcgg cctggtggcg atcgcgttcg ggatcctggc catgatccgg
     6361 cccggcgccg cgttcctgtc gctggtgctg ctgttcggcg tgtacgcgat cgtcgacggc
     6421 gtgctggcgc tggtgctcgc gtcgcagcgg gtccatgcgc gcggcgcgct ggtcgcgcgc
     6481 gggatcgtct cgatcgcggc cggcgtcctc gcgctcgagt ggcccgggat caccgcgttc
     6541 gtcctgctgc tcgtgatcgc ggggtgggcg atcgtgtccg gcgtcctcga gatcgcgatg
     6601 gcgatccaga tgcgcaagca gctcgagagc gagtggctgc tcgcgctcga gggcgcgctg
     6661 tcggtcgtgt tcggggtgct gctgctgctg tcgccgctgg tcggcgcgat cgtgctcggc
     6721 ctgtggatcg gcgcgtgggc gctggtgctc ggcggg
//
LOCUS       DASVOR010002166         5715 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_315769,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002166 DASVOR010000000
VERSION     DASVOR010002166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5715
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315769"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VF516_40190"
     CDS             <1..778
                     /locus_tag="VF516_40190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEX8114041.1"
                     /translation="VMLIDSHAHVDGPEFDADRADVLARARAAGVQRIVVIGAVGSPD
                     SAERAVALAERDPEIWATVATHPHDAAQMTPAWWEVHERLARHPRVVAVGETGLDYYY
                     DHSPRDVQQAAFARFIELARAVGKPVVCHIRDAHDDARAILRAGNVTDCVIHCFTGTP
                     DDARAYAELGYYVSFSGIVTYKTAQALRDAVPRVPRDRLLIETDCPYLAPVPRRGKRN
                     EPAFLPHTAEVVARCSGLSFEDLAEVTTRNACRVFRLPGA"
     gene            841..1578
                     /gene="rplC"
                     /locus_tag="VF516_40195"
     CDS             841..1578
                     /gene="rplC"
                     /locus_tag="VF516_40195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEX8114042.1"
                     /translation="MALKIGLLGKKIGMTQVFADDGEAVPVTVIQAGPCLVIGTRTAE
                     RDGYSALVLGFDEKPVRLASKPELGAGKDTGIKPQRFVRELRLPAEEVAKFKVGQVLG
                     PKDVFADNTAIDVEGTSKGKGYQGVIKRHHMAGMTRAHGTHEYFRHGGSIGCRLTPQR
                     VHKGKRMAGHMGHEKKSVQNLQLLRIMADDNVILVRGSVPGAANDYVVVTNAATRTAY
                     KRKGVGKEEVRSKNPLKASKKAAAGRG"
     gene            1790..2440
                     /locus_tag="VF516_40200"
     CDS             1790..2440
                     /locus_tag="VF516_40200"
                     /inference="COORDINATES: protein motif:HMM:NF013856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEX8114043.1"
                     /translation="MAKLDDRSTRRDRFHQKAKREGFLARAVYKLEEIDGRFGIFERG
                     MTRVLDLGCAPGSWLQYARQRIGEHAQLVGLDRGPLHRPPPGARIIVGDVMTIPFTEL
                     LGELLGELPAFDVVLSDMAPDTTGIRHLDQARSEALFERALEIAQAVLAPGGNFVGKL
                     FQGPDFKRLTEAVRARFAVQRTAKPASSRQISIEQYVIGKGFRGAGARTGPGGAAP"
     gene            2437..3072
                     /locus_tag="VF516_40205"
     CDS             2437..3072
                     /locus_tag="VF516_40205"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEX8114044.1"
                     /translation="MSSPLRLVIDRWGPPSPRKIEAAVEVLHRGGVAAYPTDSIYALG
                     CAIEARDAIERIYRAKGMKKNQRLALICPDLSTASEFGMFSQTAFRLAQRIFPGPYTL
                     VVPATRSVPRTLTDAKRRTVGIRVTGHPIAQALAVALGRPLLTTSAIAPATGEPCRDA
                     DEILEAFGKHIDVVIDTEQTPAAPSTVIEVDGDNITLIREGQGPIAGILDV"
     gene            complement(3091..3495)
                     /locus_tag="VF516_40210"
     CDS             complement(3091..3495)
                     /locus_tag="VF516_40210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114045.1"
                     /translation="MKALAVMVFAVCISACGAIQVDGDPGSDSPVVVDLSSHEQRIGD
                     WVVEGRDPAPPDAVSDAVPRPSAAGPVSCVVIQYCDAPGPDGTRCVQKGCSVQEALTE
                     CTIEAPRECHPLVCPFVFLGHGGVRFNNGECM"
     gene            complement(3763..>5715)
                     /locus_tag="VF516_40215"
     CDS             complement(3763..>5715)
                     /locus_tag="VF516_40215"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEX8114046.1"
                     /translation="VVGYIDTGIDPDSCYFRDTARGLPTTNACNGGTAVNTAQRKVLA
                     VDFLTSSECAGGIGNTEWDTQGHGSHVAGTIAGDNFANPITHDPGDGMAPGAKLVVQD
                     AGFRTDNCGDLPGIGCPVVDLKPIFQQAYTQGARIHTNSWGDNENAAVQNNYSAASED
                     VDQFMFDHPDMLILFAAGNAGPGTGSVGSPSTNKNGLSVGATLRGTSANSMASFSSCG
                     PTADGRIKPDLTMPGSNIVSARNDTNVTSNNCATTTMSGTSMASPAAAGMAALVRQYY
                     TDGFYPTGAAVAANRIAAPSAALVKATLLNSTQVMTGTGTGPVPNSCQGWGRVLLDNA
                     LFFAGQTRTLFAADDPGFAQGAAGQTRTFTLQVAAGQPLRVTLAWTDFPSTPAASVNL
                     NNDLDLVVSGPSGTFLGNVFASSQSTTGGTADRRNNIEQVAINAPAAGSYTITVRAFS
                     VPSSAQPFALVASGAIGTGTGPQVVFSDDFETDKGWIANPAGTDTATTGRWERAVPQA
                     TTSTGTKQLGTANSGTHDLVTGAAAGAAAGDNDIDGGVTTIQSPVIAIPAGGTVTLSL
                     AFYFAHLSNSSSDDFFRVQIVGAAAQTVIEELGSAVNDNAAWVTRSVDISAFAGQSVR
                     ILISAADNAAGSLVEAGIDDVVITRQ"
BASE COUNT          798 a         2040 c         2044 g          833 t
ORIGIN      
        1 cgtgatgctg atcgactcgc acgcccacgt cgacggcccg gagttcgacg ccgatcgcgc
       61 ggacgtcctc gcccgcgcgc gcgccgccgg ggtccagcgc atcgtggtga tcggcgcggt
      121 cggcagcccg gacagcgccg agcgcgcggt cgcgctggcc gagcgcgatc ccgagatctg
      181 ggcgaccgtg gcgacccacc cgcacgacgc cgcgcagatg acgccggcgt ggtgggaggt
      241 ccacgagcgg ctcgcccgcc acccgcgcgt ggtcgcggtc ggcgagaccg gcctcgacta
      301 ctactacgat cactcgccgc gcgacgtgca gcaggcggcg ttcgcgcggt tcatcgagct
      361 cgcgcgcgcg gtcggcaagc cggtcgtctg ccacatccgc gacgcccacg acgacgcccg
      421 ggcgatcctg cgcgccggca acgtcaccga ctgcgtgatc cactgcttca ccggcacgcc
      481 ggacgacgcc cgcgcctacg ccgagctcgg ctactacgtg tcgttctcgg gcatcgtcac
      541 ctacaagacc gcgcaggcgc tgcgcgacgc ggtgccgcgg gtgccgcgcg atcggctgct
      601 gatcgagacc gactgcccct acctcgcgcc ggtcccgcgc cgcggcaagc gcaacgagcc
      661 ggcgttcttg ccccacacgg ccgaggtggt ggcccgctgt tccggcttga gtttcgagga
      721 cttggccgag gtcacgacgc gcaacgcgtg ccgggtgttc cgcctgccag gcgcttgacg
      781 caccccggcg ggacaggtat agattccgcc ttcgtttcgg tagcgtagga gctgtttgcc
      841 atggcactca agatcggcct gctcggcaag aaaatcggta tgacccaggt gttcgcggac
      901 gacggcgagg ccgtcccggt caccgtgatc caggccggtc cgtgcctcgt gatcggcacc
      961 cgcaccgccg agcgcgacgg gtactcggcg ctcgtgctcg gcttcgacga gaagccggtg
     1021 cggctcgcca gcaagcccga gctcggcgcg ggcaaggaca ccgggatcaa gccgcagcgc
     1081 ttcgtccgcg agctccggct gcccgccgag gaggtcgcca agttcaaggt cggccaggtg
     1141 ctcgggccca aggacgtgtt cgccgacaac acggcgatcg acgtcgaggg caccagcaag
     1201 ggcaagggat accagggcgt gatcaagcgc caccacatgg cgggcatgac ccgtgcccac
     1261 ggcacccacg agtacttccg ccacggcggg tcgatcggct gccggctgac cccgcagcgc
     1321 gtccacaagg gcaagcgcat ggcgggccac atgggccacg agaagaagtc ggtccagaac
     1381 ctccagctgc tccgcatcat ggcggacgac aacgtcatcc tcgtccgcgg ctcggtgccc
     1441 ggcgccgcca acgactacgt ggtggtgacc aacgcggcga cccgcaccgc gtacaagcgc
     1501 aagggtgtcg gcaaggaaga ggtccggtcg aagaacccgc tcaaggcgtc caagaaggcc
     1561 gctgccggtc gcgggtgatc ggatcttgat cttgatcgtt atcgctatct aaccccggcg
     1621 gccggcggcg cccggcgtgg cgctcgcagc ctgcgcggcg tgtacgcagg ctcgcgcagc
     1681 catgcgtgtc ggaagcgcac cagctgcgtg gcgagcgccc gcgcgcgggg atcgcctgcg
     1741 gaacacttgg cttctgccgc catcgtggtg aactctcccc gctccgcgta tggccaagct
     1801 cgatgaccgc agcacgcgcc gcgatcgctt tcaccagaag gcgaagcgcg agggcttcct
     1861 cgcgcgcgcg gtgtacaagc tggaggagat cgacggccgg ttcgggatct tcgagcgcgg
     1921 gatgacgcgg gtgctcgatc tcggctgcgc gccggggtca tggctgcagt acgcgcggca
     1981 gcggatcggc gagcatgcgc agctggtcgg gctcgaccgc gggccgctgc accggccgcc
     2041 gccgggcgcg cggatcatcg tcggcgacgt catgacgatc ccgttcaccg agctccttgg
     2101 cgagctgctc ggcgagctgc cggcgttcga cgtcgtgctg agcgacatgg cgcccgacac
     2161 caccgggatc cgccacctcg accaggcgcg gtccgaggcg ctgttcgagc gcgcgctgga
     2221 gatcgcccag gccgtgctgg cgccgggcgg caacttcgtc ggcaagctgt tccagggccc
     2281 ggacttcaag cggctgaccg aggcggtgcg cgcgcggttc gcggtgcagc ggaccgccaa
     2341 gccggcgtcg agccggcaga tctcgatcga gcagtacgtg atcggcaagg ggttccgcgg
     2401 cgcgggcgcg cgaaccggtc cgggaggcgc ggcgccgtga gctcgccgct gcgcctcgtg
     2461 atcgaccgct ggggcccgcc gtcgccgcgc aagatcgagg ccgcggtcga ggtgctccat
     2521 cgcggcgggg tcgccgccta ccccaccgac tcgatctacg cgctgggctg cgcgatcgag
     2581 gcgcgcgacg cgatcgagcg gatctaccgc gccaagggca tgaagaagaa ccagcggctc
     2641 gcgctgatct gccccgacct gtcgaccgcg tcggagttcg gcatgttctc gcagaccgcg
     2701 ttccggctcg cccagcggat cttccccggg ccctacacgc tggtcgtgcc ggcgacccgc
     2761 agcgtcccgc gcacgctgac cgacgccaag cgccgcacgg tcggcatccg ggtcaccggc
     2821 cacccgatcg cccaggcgct cgccgtggcg ctcggccggc cgctgctcac caccagcgcg
     2881 atcgcgccgg cgaccggcga gccgtgccgc gacgccgacg agatcctcga ggcgttcggc
     2941 aagcacatcg acgtcgtgat cgacaccgag cagaccccgg ccgcgccgtc gacggtgatc
     3001 gaggtcgacg gcgacaacat cacgctgatc cgcgagggcc agggcccgat cgccggcatc
     3061 ctcgacgtgt gagcctggcg ccgctgcgcg tcacatgcat tccccgttgt tgaaccggac
     3121 cccgccgtgc ccgaggaaca cgaacgggca caccaggggg tggcactcgc gcggcgcctc
     3181 gatcgtgcac tcggtcagcg cctcctgcac gctgcagccc ttctgcacgc agcgcgtgcc
     3241 gtcgggcccc ggggcatcgc agtactggat gaccacgcag gacacgggcc cggccgcgct
     3301 cggccgcggc acggcgtcgg acaccgcgtc cggcggcgcc gggtcccggc cctcgaccac
     3361 ccagtcaccg atccgctgct cgtgggacga caggtccacc accaccgggc tgtcgctgcc
     3421 cggatcgccg tcgacctgaa tcgctccaca cgccgagata cagactgcaa acaccatcac
     3481 cgcgagcgcc ttcattgaat tctccctttg ttgccggcaa ccctgggctg gcaccgcgcg
     3541 gccatcgagc aagctcacgt gcggctcact cgctgcgggc tcccaccggt cgcgcatggt
     3601 gtccggcggc cggacactcg ccgccagccc gcgatcgagc ccgtgataga cgcgcaggcg
     3661 agctcacaga tctgcgcggc gccgtcgagc cacgccccgc cggacgcggg ccgaaacgtg
     3721 cgccggcgcc cggccggggc agccttgtcg cctcgttacc ggctattgcc gcgtgatgac
     3781 gacatcgtcg atcccggcct cgaccaggct gccggcggcg ttgtcggcgg ccgagatcag
     3841 gatgcgcacc gactggccgg cgaacgccga gatgtcgacc gagcgggtga cccacgcggc
     3901 gttgtcgttg accgcgctgc cgagctcctc gatgaccgtc tgggcagccg cgccgacgat
     3961 ctggacccgg aagaagtcgt cgctcgacga gttgctcagg tgcgcgaagt agaacgcgag
     4021 cgacagcgtc accgtcccgc cggcggggat cgcgatcacc ggcgactgga tcgtggtcac
     4081 gccgccgtcg atgtcgttgt ccccggcggc ggcgcccgcc gcggcgccgg tcaccagatc
     4141 gtgcgtgccg ctgttggcgg tcccgagctg cttggtgccg gtgctggtcg tggcctgcgg
     4201 gaccgcgcgc tcccagcgcc cggtggtcgc ggtgtcggtg ccggcggggt tggcgatcca
     4261 gcccttgtcg gtctcgaagt cgtcgctgaa cacgacctgc ggcccggtgc cggtgccgat
     4321 cgcgccggac gcgaccagcg cgaacggctg cgccgagctc ggcacgctga acgcgcgcac
     4381 cgtgatcgtg tagctgcccg ccgcgggcgc gttgatcgcg acctgctcga tgttgttgcg
     4441 gcggtcggcg gtgccgccgg tggtcgactg gctgctcgcg aacacgttgc cgaggaacgt
     4501 ccccgacggc cccgacacca cgaggtcgag atcgttgttg aggttcacgc tggcggccgg
     4561 cgtcgacggg aagtcggtcc acgccaacgt cacgcgcagc ggctggccgg ccgcgacctg
     4621 gagcgtgaac gtcctggtct ggccggcggc gccctgcgcg aacccgggat cgtcggcggc
     4681 gaacagcgtg cgggtctggc cggcgaagaa cagcgcgttg tcgagcagga cccggcccca
     4741 gccctggcag ctgttgggca ccgggccggt gccggtgccg gtcatcacct gggtcgagtt
     4801 gagcagcgtg gccttgacca gcgcggcgct gggcgccgcg atcctgttcg ccgccaccgc
     4861 cgcgccggtc gggtagaacc cgtcggtgta gtactggcgg accagcgccg ccatgccggc
     4921 cgcggccggg ctcgccatgc tggtcccgct catcgtcgtg gtcgcgcagt tgttcgacgt
     4981 cacgttggtg tcgttgcgcg ccgacacgat gttcgagccg ggcatcgtga gatccggctt
     5041 gatccggccg tcggccgtcg gcccgcagct cgagaagctc gccatcgagt tggccgacgt
     5101 gccgcgcagg gtcgcgccga ccgagagccc gttcttgttg gtcgacgggc tgccgacgct
     5161 gccggtcccc ggcccggcgt tgccggcggc gaacaggatc agcatgtcgg ggtggtcgaa
     5221 catgaactga tcgacgtcct cggacgccgc cgagtagttg ttctggaccg cggcgttctc
     5281 gttgtcgccc cacgagttgg tgtggatccg cgcgccctgc gtgtaggcct gctggaagat
     5341 cggcttgaga tcgacgaccg ggcagccgat gccggggaga tcgccgcagt tgtcggtgcg
     5401 gaacccggcg tcctggacga ccagcttggc gccgggcgcc atgccgtcac cggggtcgtg
     5461 ggtgatcggg ttggcgaagt tgtcgccggc gatcgtgccc gcgacgtgcg agccgtggcc
     5521 ctgggtgtcc cactcggtgt tgccgatgcc acccgcgcac tcgctcgagg tcaggaagtc
     5581 gaccgcgagc accttgcgct gcgcggtgtt gaccgcggtg ccgccgttgc acgcgttggt
     5641 cgtcggcagg ccgcgcgcgg tgtcgcggaa gtagcacgag tcggggtcga tgccggtgtc
     5701 gatgtagccg acgac
//
LOCUS       DASVOR010002167         2950 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_81871,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002167 DASVOR010000000
VERSION     DASVOR010002167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81871"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..981
                     /locus_tag="VF516_40220"
     CDS             157..981
                     /locus_tag="VF516_40220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114047.1"
                     /translation="MDFGAVAAAPSELLKLVLIEFATVYGNDWFTLPLDALPVGSLCE
                     IGAVTVTDAFGVSINLAPFGDGAGTDWRMFELSRDDGAANAGNALLLLDSLPTTLESG
                     PIEEVVMLRDELANMAWAVEKVVESRTGRPLDRHEDEVTRRATPAPPELTAARRYLLQ
                     TAVPRNWIPLLPKLDGDTSGATILRRLARGAMRDPASGSPIPPLGRLLEPGTPLDIYD
                     EELPRTGLRATRLWTLGRASNGRTHLWRGRQKGPGRGEASSGLRFDGTLTGRTSKS"
     gene            1031..2251
                     /locus_tag="VF516_40225"
     CDS             1031..2251
                     /locus_tag="VF516_40225"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8114048.1"
                     /translation="MTEPETFRTELAEFLDQELPRQLPDLPPITAEYWGGRRPELSHP
                     SSRRYCELMAERGLTAPTWPREYGGGGLDTERAQIVVAELRRRRLPPPLSGFGLTMIG
                     PTLLQFGTDDQKREHLPKIVRGEIRWAQGYSEPNAGSDLASLAASARIVGDEIALDGQ
                     KIWTSYADKADWVFGIFRTDTGGKKQEGITFLLLDMAQPGVEVRPIKLISGASPFCEM
                     FFTDARAHTRDIIHGVGNGWTVAKALLGHERTMIADVFGPSGRGRDVTAGNPLVDLAR
                     DYVGEREREREPGGQIADPVLRDRVTQVTMDERCFALTMQRSADAARAGHRPGPESAI
                     FKIYGSELNQRRHELMLALRGPQCLGWEGDGFDDPELAQTREWLFSRGITIAGGTSEI
                     QLNIIAKHVLALPD"
     gene            2357..2749
                     /locus_tag="VF516_40230"
     CDS             2357..2749
                     /locus_tag="VF516_40230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114049.1"
                     /translation="MKTLDHWKNYEPIADVATHDAQGAGPIARTLQVTAHVAGSDLAA
                     PLGLTIAGLCHLALTRTQLSALRRVLARAELDLRTVAQQPVVAARTEGRCEICSRLIV
                     TGDLVYVIDDGGGEHVLAHAERCPGPSA"
BASE COUNT          461 a         1098 c          977 g          414 t
ORIGIN      
        1 gactggtact cgttcgtgcg acgcgcgccg ggctcgacgc tgggtgcgcc tgccgcaggg
       61 cggggtccga tcgccttcac ccgcagcgcc attcccaacc cggtcagcta tcccgacatg
      121 ccggtgcccc gctagtggga gctcgaggac cggcgcgtgg acttcggcgc cgtcgcggcc
      181 gcaccgagcg agctgctcaa gctcgtgctc atcgagttcg ccaccgtgta tggcaatgac
      241 tggttcacgc tgccgctcga cgctttaccg gtcggaagcc tgtgcgagat cggcgcggtg
      301 acggtcaccg acgcgtttgg cgtctcgatc aaccttgccc cgtttggcga tggcgcgggc
      361 acggactggc gcatgttcga gctgtccaga gacgacggtg cggccaatgc gggcaacgcc
      421 ctgctgttgc tcgactcgct gccgacgaca ctggaatccg gcccgatcga ggaagtggtc
      481 atgctccgcg acgagctggc gaacatggcc tgggcggtgg agaaggtcgt cgagagccgc
      541 accggacgtc cgctcgaccg ccacgaagac gaggtcaccc gccgcgcaac gccggcacca
      601 cccgagctca ccgccgctcg ccgctatctg ctgcagaccg ccgtcccccg caactggatc
      661 ccgctgcttc ccaagctcga cggcgacacc agcggcgcga cgatcctgcg ccggcttgcc
      721 cgcggtgcga tgcgagatcc agcgagtgga tcacccatcc caccgctcgg acgactgctc
      781 gaacctggta cgcctctcga catctacgat gaggagctgc cgcgcaccgg cttgcgcgcg
      841 acccggctct ggacgctcgg ccgcgcatcc aatggccgca cccacctgtg gcgtgggaga
      901 cagaagggcc ccgggcgcgg tgaagcgtcg agcggcctgc gtttcgatgg caccctgacg
      961 ggacgaacca gcaagagcta ggatctcccc ggcttgaccg gggtgcgctt tggcggttcc
     1021 atccggcgcg atgaccgagc cagagacgtt ccggaccgag ctcgccgagt tcctggacca
     1081 ggagctgccg cgccagctcc ccgacctgcc ccccatcacc gccgagtact ggggtggccg
     1141 ccggccggag ctctcgcacc cgagctcccg gcgctactgc gagctgatgg ccgagcgcgg
     1201 actgaccgcg ccgacctggc cgcgagagta cggcggcggc gggctcgaca cggagcgcgc
     1261 gcagatcgtg gtcgccgagc tgcgccggcg gcgcctgcca cctccgctga gcggcttcgg
     1321 gctgacgatg atcggcccga ccctgctcca gttcggcacc gacgaccaga agcgcgagca
     1381 cctgccgaag atcgtgcgcg gcgagatccg ctgggcgcag ggctactccg agcccaacgc
     1441 cggcagcgat ctcgcgtcgc tcgcggcctc ggcgcggatc gtcggcgacg agatcgccct
     1501 cgacggccag aagatctgga ccagctacgc cgacaaggcc gactgggtgt tcggcatctt
     1561 ccgcaccgac accggcggca agaagcagga gggcatcacg ttcctcctgc tcgacatggc
     1621 acagcccggc gtcgaggtcc ggccgatcaa gctgatcagc ggagcgtcgc cgttctgcga
     1681 gatgttcttc accgacgcgc gggcgcacac ccgcgacatc atccacggcg tcggcaacgg
     1741 ctggaccgtg gccaaggcgc tgctcggcca cgagcgcacc atgatcgccg acgtgttcgg
     1801 ccccagcgga cgcgggcgcg atgtcaccgc gggcaacccg ctggtcgacc tcgcgcgcga
     1861 ctacgtcggc gagcgcgagc gcgagcgcga gccgggcggc cagatcgccg atccggtgct
     1921 gcgcgaccgc gtcacccagg tcaccatgga cgagcggtgc ttcgcgctga ccatgcagcg
     1981 cagcgccgac gccgcccggg ccgggcacag gcccggaccc gaatcggcga tcttcaagat
     2041 ctacggcagc gagctcaacc agcgccggca cgagctgatg ctcgcgctgc gcgggccgca
     2101 gtgcctgggc tgggaaggcg acggcttcga cgatcccgag ctcgcccaga cccgcgagtg
     2161 gctgttctcc cgcggcatca ccatcgccgg cggtacgagc gagatccagc tcaacatcat
     2221 cgccaagcac gtgctcgcgc tcccggactg atccacggct cgctgccgcc gggccgccga
     2281 tggcgctccc gcacccgaac gcgacgcgct agcgttccga ccggatctcg gctacccggg
     2341 agccgatcgc ccgctcatga aaaccctcga ccactggaag aactacgagc ccatcgccga
     2401 cgtcgccacc cacgacgccc agggcgccgg gccgatcgct cgcaccctgc aggtcaccgc
     2461 gcacgtggcc gggagcgacc tcgccgcgcc gctcggcctc acgatcgccg ggctctgcca
     2521 cctcgcgctc acgcgcacgc agctctcggc gctccgccgg gtcctcgcgc gggccgagct
     2581 cgatctccgg accgtcgcgc agcagccggt cgtcgcggcc cggaccgagg gccgctgcga
     2641 gatctgctca cggctgatcg tcaccggcga cctcgtgtac gtgatcgacg atggcggcgg
     2701 cgagcacgtc ctggcgcacg ccgagcgctg cccggggccg tccgcatgag cggcgctttg
     2761 accgctgcgc actcgacatc acgatgtccc gctccgtcgt cgccaccatc ctcatctttg
     2821 caacacctgc gatgcgagaa tctctcacct gccccgacgt gcaacgtgag tgttccgtga
     2881 agacaacaga gaaccgctcg acggcctgcg cctcggtccc gtagggatgg acgacgcagt
     2941 attccgtgaa
//
LOCUS       DASVOR010002168         1707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_169211,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002168 DASVOR010000000
VERSION     DASVOR010002168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169211"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..758
                     /locus_tag="VF516_40235"
     CDS             <1..758
                     /locus_tag="VF516_40235"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF006050.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEX8114050.1"
                     /translation="PSAGNAAGARAAYGAAARLPVVVAMPEDTPRTFVDECRHYGAEV
                     HLTPGTIADAGRWLREHGPRDSFDVSTLKEPYRIEGKKTMAYELWEQLSGALPDAIIY
                     PTGGGTGLVGMWKAFSEMRALGWPMPRIPRLISVQMAGCAPVVRGYVEGAAATAAWPD
                     PRTRVWGLRVPSPLGGFLCLRAVRETKGTALAITESDAAAATSRLAARTGLDICPEGG
                     AAFAAYETLRERGEIAPHETVVVFNTGTGLKYR"
BASE COUNT          285 a          642 c          530 g          250 t
ORIGIN      
        1 ccccgtccgc cggcaacgcc gccggcgccc gcgccgccta cggcgccgcc gcccgcctcc
       61 ccgtcgtcgt cgcgatgccc gaggacaccc cgcgcacgtt cgtcgacgag tgccgccact
      121 acggcgccga ggtccacctc acccccggca cgatcgccga cgccggccgc tggctgcgcg
      181 agcacggccc gcgcgacagc ttcgacgtct ccaccctcaa ggagccgtac cggatcgagg
      241 gcaagaagac catggcctac gagctgtggg agcagctgtc cggcgccctg cccgacgcca
      301 tcatctaccc caccggcggc ggcaccggcc tggtcggcat gtggaaggcg ttctccgaga
      361 tgcgcgccct gggctggccg atgccccgca tcccgcgcct gatctcggtc cagatggccg
      421 gctgcgcccc ggtcgtgcgc ggctatgtcg agggcgccgc cgccaccgcc gcctggcccg
      481 atcccaggac ccgggtctgg ggcctgcgcg tcccctcgcc gctcggcggc ttcctctgcc
      541 tgcgcgccgt ccgcgagacc aagggcaccg ccctcgccat caccgagagc gacgccgcgg
      601 ccgccacctc gcgcctcgcc gcccgcaccg gcctcgacat ctgccccgag ggcggcgccg
      661 cgttcgccgc ctacgagacc ctgcgcgagc gcggcgagat cgccccgcac gagaccgtcg
      721 tcgtgttcaa caccggcacc ggcctcaagt accgctgagc gtgccagagc ccgcgctcag
      781 acgctcgaat gctctccgag cggcgcggcg aggcacctca tgcaatcaac ccgctccgat
      841 gactgacgat cgactggcca tcgtggctct cccagctttg cacgacacag cggcgcgcct
      901 ccccgcattg catgaatgac gtcgaggctg tcgcgaacgg tttcccaagg cagtgcgatc
      961 gcgcaggtgc cacgggccga tccacgtggg accagggcac cgcagcttgg ctgggcgggc
     1021 agcttcgtgc cgatgctgcg ccaccgcatg gcgaagccga gctgtgcctc gtcgacgaac
     1081 cctcgagcgg agttccccgc agaacctcgg gcggcctgat ggagaacagg gacgtctgat
     1141 gacccgcgcc gcggaccacc cgatcgaccc atacgcggct cacagccgtc ctgctgttcc
     1201 cccaggcctg gccctcgacg cacgctcgct ggaagatgat ttttatggat cttgttcaga
     1261 agacgtcagc tgtttcagtc cacgagtatg attgttcgac ggatggatca atcactctac
     1321 caaccatggg ttgacttctc gcgctgacct gacgatatag aaatgttcct ccggaacaag
     1381 gcagcgccgc cgtaggcgca aggcatgacc atagcgggcc accgggaaca ccgacgaact
     1441 tccaagcaga ccgaggcacc cgcggtattc cgccagcgct cgacggtgca agctgggcga
     1501 ggccggaagg tacgcctggc tccccgccga tgcctcttct ccggatcccc caaaagatca
     1561 attccggagt gcgaccagga catgttggtc cgtaaggaac aggaggtgcg acgtccttca
     1621 agaaatacgg aagcgtcatc ttgcgggtgc gaatcccgcc cgacgaagcc agccaggcac
     1681 gtcggtagcg aaccttgcga cgggcat
//
LOCUS       DASVOR010002169         5030 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_374040,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002169 DASVOR010000000
VERSION     DASVOR010002169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5030
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374040"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1842
                     /locus_tag="VF516_40240"
     CDS             <1..1842
                     /locus_tag="VF516_40240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114051.1"
                     /translation="EDKAEHASEEFAIPDSGGMRVLISTKPWQSGAGGGAPFAGGAGG
                     MPNPRSMSGEPRGEQADPPGMITVRVTYNDFKDSPAGVPVAMVGYSAADSVSYQVQNT
                     DSEGRVKFTGLDRTGGTSYFAMALLPRNGAVDRLISTHVVLESQVGVRMVLSSEKRDS
                     TAPAIDDFAKVDAPIATPAGKVRVVLEGIGDLKAKVTLVDAATRKVLGEAKPESAPDP
                     SRVQAEPRFSSDPNLPAGTLDVEVVGGPGQEEEPLRDLAVRVLPASSNDATGGVASAT
                     GADGLVRMAVPASEPQKVVVTINGRQLVSPPVDLTKSGGRLVIRAHWTDSGRPQALFD
                     VAGTQGQVVYAECDDRGQHYRSMPFQLLDAAGTKVTIYVFPRVLFRFQLDADAEDEQL
                     AVRGKFEVTNYSWAPYRAGPDGLLVPMPHGFKGAILADSDQAEVSVVPGEGYRIIRPI
                     PPGGRPFRAAFYLPVDAGQAAWALDLPLGAFESELVIKQNPGMTLQTPPSVHAESRTV
                     PQGTFYILGPITIHRAQSMTMAIEGLPSTPRWRSWVQGIVGVLVVATILGGLLFALLA
                     RRPAQAADAHAEARRQRLLDELVELERSGANPKRREQLLAELEQLWG"
     gene            1853..2587
                     /locus_tag="VF516_40245"
     CDS             1853..2587
                     /locus_tag="VF516_40245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8114052.1"
                     /translation="MAAPQQREPGGIDQVQIRKVAKRYGSERALAGVSLDLAAGSMCA
                     LLGHNGAGKTTLLGIVSTLVRPSAGSVAYRSRGSALTGVAVRREIGMLAHASLCYGEL
                     TARENLALIAGLYEIDGSAAGVDGVLDRVGLDRKARDRAARTYSRGMLQRLALARALL
                     TRPSLLLLDEPFTGLDRDGALSLGEQLGQLRDAGAIVVVVTHDLEAIAGRTDHVAILR
                     RGQLVFEERGAYTYEQLKDVYHHHAN"
     gene            2719..3402
                     /locus_tag="VF516_40250"
     CDS             2719..3402
                     /locus_tag="VF516_40250"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEX8114053.1"
                     /translation="MSSLVRHAARIAWKDLRIELRSREILYTMVFFGALLVVIFAVAF
                     PRDARAVRGSLPGMLWTAIAFTGTIGLGRAFDRERENDTMRALLLAPVPRLAVFLGKA
                     IATTLLVLAVSVVCAPLLAVFLDVPLFAYPLPLALIVVLGAIGIAVTGSVFAATLLKV
                     RSRDVLLPVILYPLLIPLFGAGTKCTAALIADPPNLEGAWYWIQFLGIYDAAFLVVSV
                     WIFESLVIE"
     gene            3447..4262
                     /gene="ccsA"
                     /locus_tag="VF516_40255"
     CDS             3447..4262
                     /gene="ccsA"
                     /locus_tag="VF516_40255"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX8114054.1"
                     /translation="MNKPTTPVFAVLAPVFAVLAAVGIAKSLYSIFFQAPLQYGLNDD
                     GKLNGSSLFFNQKIFYFHTAHAMLMILAVVSAGVASILFLLSRTAPDAHAAPGAEPDG
                     LLSAVVRFVRSHGSTWDDLASASVDVAVAFGAIALLSGSIWAKAAWDVWWNWEPRLTI
                     TLLLWLVLVGYVVVRRFAGPSADRIAAGMAIFGGVTCWFVYTMVGKDSHPAAGSNGVV
                     ATLGPGMKGTFWLSVASFTCWYIALVVSRVQSTRAERELRELRERGLDLGVLQ"
     gene            4259..4693
                     /locus_tag="VF516_40260"
     CDS             4259..4693
                     /locus_tag="VF516_40260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114055.1"
                     /translation="MTHSLTKLIAPWLVIAGLAGAAAAQPAGSGAAPPAAPADATGAR
                     AQCTAAMNADPKFAADIVKIVDENAAIKRDQDTLNAHNDAYAHVQKNERHVIYAYAVM
                     WIIAAAFVIFLWRRQQALQAEIAQLHRDLDAAASDKPVGGRS"
     gene            4690..4884
                     /locus_tag="VF516_40265"
     CDS             4690..4884
                     /locus_tag="VF516_40265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114056.1"
                     /translation="MTASHVIFIPAVLMVGMFLGFIFGARAARNQFDLQQKREAERDA
                     ARAARAARRTAGSDKPSPPT"
BASE COUNT          676 a         1790 c         1816 g          748 t
ORIGIN      
        1 gaggacaagg ccgagcacgc gtccgaggag ttcgcgatcc ccgacagtgg cggcatgcgc
       61 gtcctgatct cgaccaagcc gtggcagagc ggggcgggcg gcggcgcacc gttcgcgggc
      121 ggggccggcg gcatgccgaa cccgcggtcg atgagcggcg agccgcgcgg cgagcaggcc
      181 gatccgcccg gcatgatcac cgtgcgcgtc acctacaacg acttcaagga ttcgcccgcc
      241 ggcgtgccgg tcgcgatggt cggctactcg gccgccgact cggtcagcta ccaggtgcag
      301 aacaccgaca gcgagggccg ggtcaagttc accggcctcg atcgcaccgg cgggacctcg
      361 tacttcgcga tggcgctgct gccgcgcaac ggcgcggtcg atcggctgat ctcgacccat
      421 gtggtgctgg agtcccaggt cggggtgcgc atggtgctgt cgagcgagaa gcgcgactcg
      481 acggcgccgg cgatcgacga cttcgccaag gtcgatgcgc cgatcgcgac gccggccggc
      541 aaggtccggg tcgtgctcga gggcatcggc gacctcaagg ccaaggtgac gctggtcgac
      601 gcggcgaccc ggaaggtgct gggcgaggca aagccggagt cggcgccgga tccgtcgcgc
      661 gtgcaggccg agccgcggtt ctcatcggat cccaacctgc cggccggcac gctcgacgtc
      721 gaggtcgtcg gcgggcccgg gcaggaggag gagccgctgc gcgatctggc ggtccgggtg
      781 ctgccggcga gcagcaacga cgcgaccggc ggggtggcct cggcgaccgg cgccgacggc
      841 ctggtgcgca tggcggtccc ggcgagcgag ccgcagaagg tcgtggtgac gatcaacggc
      901 cgccagctgg tgtcgccgcc ggtcgatctg accaagtcgg gcggcaggct ggtgatccgc
      961 gcgcactgga ccgacagcgg ccggccgcag gcgctgttcg acgtcgccgg gacccagggc
     1021 caggtggtct acgccgagtg cgacgaccgc ggccagcact accgctcgat gccgttccag
     1081 ctgctcgacg ccgccggcac caaggtcacg atctacgtgt tcccgcgcgt gctgttccgg
     1141 ttccagctcg acgccgacgc cgaggacgag cagctcgcgg tgcgcggcaa gttcgaggtc
     1201 accaactact cgtgggcgcc gtaccgcgcg gggcccgacg gcctgctcgt gccgatgccg
     1261 catggcttca agggcgcgat cctcgcggac agcgaccagg ccgaggtctc ggtcgtgccg
     1321 ggcgagggct accggatcat ccgtccgatc ccgccgggcg gccgcccgtt ccgcgcggcg
     1381 ttctacctgc cggtcgacgc cggccaggcc gcgtgggcgc tcgacctgcc gctcggcgcg
     1441 ttcgagagcg agctcgtgat caagcagaac cccgggatga cgctgcagac cccgccgagc
     1501 gtccacgccg agagccggac cgtgccgcag gggacgttct acatcctggg gccgatcacg
     1561 atccacaggg cccagtcgat gacgatggcg atcgagggcc tgccgtcgac gccccggtgg
     1621 cgcagctggg tgcagggcat cgtcggcgtg ctggtggtcg ccacgatcct gggcggcctg
     1681 ctgttcgcgc tgctcgcccg ccgtccggcg caggcggccg acgcccacgc cgaggcgcgc
     1741 cgccagcggc tgctcgacga gctggtcgag ctcgagcggt ccggcgcgaa cccgaagcgc
     1801 cgcgaacagc tgctcgccga gctcgagcag ctgtggggct agccgaggcg gcgtggcggc
     1861 gccccagcag agagagcccg gcgggatcga tcaggtccag atccggaagg tcgccaagcg
     1921 ctacggcagc gagcgggcgc tcgccggcgt ctcgctcgac ctggcggccg gctcgatgtg
     1981 cgcgctgctc ggccacaacg gcgccggcaa gaccacgctg ctcggcatcg tctcgaccct
     2041 ggtccggccg agcgccggat cggtcgcgta tcgctcgcgc ggcagcgcgt tgaccggcgt
     2101 cgcggtccgg cgcgagatcg gcatgctcgc ccacgcctcg ctgtgctacg gcgagctgac
     2161 cgcgcgcgag aacctcgcgc tgatcgccgg gctgtacgag atcgacggca gcgccgcggg
     2221 ggtcgacggc gtgctcgacc gggtcgggct cgaccgcaag gcgcgcgatc gcgcggcccg
     2281 gacctactcg cgcggcatgc tgcagcgcct cgccctggcg cgcgcgctgc tcacccggcc
     2341 gtcgctgctg ctgctcgacg agccgttcac cggcctggac cgcgacggcg cgctgtcgct
     2401 cggcgagcag ctcggccagc tgcgcgacgc cggggcgatc gtcgtggtcg tgacccacga
     2461 tctcgaggcg atcgccggcc ggaccgacca cgtcgcgatc ctgcggcgcg ggcagctggt
     2521 gttcgaggag cgcggcgcct acacctacga gcagctcaag gacgtctatc atcaccacgc
     2581 caactgaccg gccccgcgtg ctcccgtgcc ctcccggatt cccgtgctct ccgtgttccc
     2641 cttgctcgcc accgtgaccc cgcgcctgca cgattccctg cccctggccc cggccctggc
     2701 ccgggcgacg gaggtggcgt gagctcgctg gtgcgccatg ccgcgcggat cgcgtggaag
     2761 gacctgcgga tcgagctgcg cagccgcgag atcctctaca ccatggtgtt cttcggggcg
     2821 ctgctcgtcg tgatcttcgc ggtcgcgttc ccgcgcgatg cgcgcgccgt gcgcggctcg
     2881 ctgccgggca tgctgtggac cgcgatcgcg ttcaccggca cgatcggcct gggccgcgcg
     2941 ttcgaccgcg agcgcgagaa cgacaccatg cgcgcgctgc tgctcgcccc ggtgccgcgg
     3001 ctcgcggtgt tcctcggcaa ggcgatcgcg accacgctgc tggtgctcgc ggtctcggtg
     3061 gtatgcgcgc cgctgctcgc ggtgttcctc gacgtgccgc tgttcgcgta cccgctcccg
     3121 ctggcgctga tcgtcgtgct cggggcgatc gggatcgcgg tcaccggctc ggtgttcgcc
     3181 gcgaccctgc tcaaggtccg gtcgcgcgac gtgctgctcc cggtgatcct ctaccccctg
     3241 ctgatcccgc tgttcggggc gggcaccaag tgcaccgcgg ccctgatcgc cgacccgccg
     3301 aacctcgagg gggcgtggta ctggatccag ttcctcggaa tctatgacgc cgcattcctg
     3361 gtagtttcgg tgtggatctt cgaatccctc gtgatcgagt gaccggagcc aagaggatga
     3421 cgcatgccag gcccgagagg aacgccatga acaagcccac caccccggtg ttcgccgtgc
     3481 tcgccccggt gttcgccgtg ctcgccgcgg tgggcatcgc gaagtcgctc tactcgatct
     3541 tctttcaggc cccgctgcag tacgggctca acgacgacgg caagctcaac ggctccagcc
     3601 tgttcttcaa ccagaagatc ttctacttcc acaccgccca cgcgatgctg atgatcctcg
     3661 cggtcgtgtc ggcgggagtg gcctcgatcc tgttcctgct gtcccggacg gcgccggacg
     3721 cgcacgcggc gccgggcgcc gaaccggacg ggttgctgtc ggcggtcgtc cgcttcgtgc
     3781 gatcgcacgg ctcgacgtgg gacgacctcg ccagcgcgtc ggtcgacgtc gcggtcgcgt
     3841 tcggcgcgat cgcgctgctc agcggctcga tctgggccaa ggccgcgtgg gacgtgtggt
     3901 ggaactggga gccccggctg acgatcacgc tgctgttgtg gctggtcctg gtcggctacg
     3961 tcgtggtgcg gcggttcgcc gggccgagcg cggatcgcat cgcggccggc atggcgatct
     4021 tcggcggcgt cacctgctgg ttcgtctaca cgatggtcgg caaggactcg cacccggcgg
     4081 ccggcagcaa cggcgtggtc gcgacactcg gccccggcat gaaggggacg ttctggctga
     4141 gcgtcgcgag cttcacgtgc tggtacatcg cgctggtggt gagccgggtg cagagcacgc
     4201 gcgccgagcg cgagctccgc gagctccgcg agcgtggtct cgatctagga gtgctgcaat
     4261 gacccattcc ctgaccaagc tgatcgcccc gtggctcgtg atcgccgggc tcgccggcgc
     4321 ggccgccgca cagccggccg gatccggagc ggcaccgcct gcggcgcccg ccgacgcgac
     4381 cggcgcccgc gcgcagtgca cggcggcgat gaacgccgat ccgaagttcg cggcggacat
     4441 cgtcaagatc gtcgacgaga acgcggcgat caagcgcgac caggacacgc tgaacgctca
     4501 taacgacgcg tacgcgcacg tccagaagaa cgagcgccac gtgatctacg cgtacgcggt
     4561 gatgtggatc atcgccgcgg cgttcgtgat cttcctgtgg cggcgccagc aggcgctgca
     4621 ggccgagatc gcgcagctgc accgcgatct cgacgccgcc gcgtccgaca agccggtggg
     4681 ggggcggtcg tgaccgcgtc gcacgtgatc ttcatcccgg cggtgctgat ggtcgggatg
     4741 ttcctcggct tcatcttcgg cgcccgcgcc gcgcgcaacc agttcgacct ccagcagaag
     4801 cgcgaggccg agcgggacgc cgctcgcgcc gcgcgtgccg cccggcgcac cgccggatcc
     4861 gacaagccgt cgccgccgac ctgatcgcgg gggcgatgta ggcgcccgga cgcgcgcgtc
     4921 ctcgttgcag gaccaccggc aactttcacc acctggtggg agggttcacc aggcacggcc
     4981 caccagggat tccggctgcg gaaggagccg cgcttccgtc gagcatcgca
//
LOCUS       DASVOR010002170         3993 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_344143,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002170 DASVOR010000000
VERSION     DASVOR010002170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3993)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3993)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3993
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344143"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3993)
                     /locus_tag="VF516_40270"
     CDS             complement(<1..>3993)
                     /locus_tag="VF516_40270"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8114057.1"
                     /translation="LPAIQSLDRLYGQAERWYDLLGNLERQVELAEATGETVALKYRI
                     GHLWQIRLGDVARAIESYREALELDPQHAETLHALDGLVHGKIEPVMVARVLEPIYET
                     TGEFAKLVDVLEVMVAHNDDPLARVDLLHRIARLHEQMIGNTSAAFDAYARALRDDSG
                     NQQTLGHIERLAEINNSFDQLAGLYEGLAEPTLDVPRQVDLLSRLARVHEQELGDIAR
                     AIETYRRILEVEYDNKPAVLALDRLYSATAAWPALTEILRREIQLADGASAASEVAEL
                     QFRLGQTLQNQLGDRRGAVDVYREILAAQPSHGATLEALEDMFHAGHLQMEIGQVVEP
                     LYETAGEYGKLHGIHEVTLGKLSGADKSAMYQRLAELAETKLYDQPKAFHWWSEALAL
                     DPRWDHAVEEAERLAGATGAWDEMVAAYTRALERTKDKDVKRTTLLRMARVYETEIGD
                     PARAVETHLRVLEIEPKDEGALAALDRLYLGAGMYDDLAEILRRRIEVAGDPDEQIEL
                     YFRRGAIFADALGDLDEALACYVAVLDQESRNRKALEAIEAIHFRREAWPRLYETYEK
                     LIDVADGDDEMADIYARMARISSDALLTPGFGEDKAIELWNRVLDIRGEDPQALEALG
                     ELCTRRERWEELVEIIERQIAATQGDQDQIRLYKQLGRVWEDKLARERNALDAWLAAD
                     RLDGNDLETLRSLARLYRSTQSWDELSQTLRRIIEVGQVTGAIDESETIELYAQLGQL
                     EGEVLGRVDDAVDAWRRVIAIDPSDFRALAALEALFTREGRWEEAIDVLEKRALVLDD
                     EVQRRETLLQAAATWEEKVEELTRAAAVYERVRASDPSNATASDRLEAIYTQQYKWTE
                     LVEVLLERSELRPSVEEQLAILNRVARIYESEIGDQESAFYVLQAAFKRDYAHDQTAH
                     ELERLATATNRWQELLEEYTNRVNELEHEDRGSAADLWVKIGRWYAEHLSHLEYAIHS
                     VQQALRIDPSHTGALGGMAELQRKRGSWSELIETLQRHAAVEPSPEKKTELYIQLAEL
                     LERQMQDAAGAIHAYQQALVYDPASRGALAALDRLYRRTEQWEPLIDVMSRRAELETD
                     DASIVKLRLEVGQIWDLRLFDAGQAINAYQAVLQVDPTNLTALRALEALYEKTGQSEK
                     YLEVLEAQLDASPSDAEQIALYERMAAAWEERFGKLDRAAESLEKIVALDNRNYNAYR
                     ELARLYQQAGKWEALVETYRNHIMATADVQTRVDLYVAMGVTYDQNLNDVDRAIEAYA
                     DVLGLEPDEQRALDALGRLYEKISEWDRGVDMMAHLTTLTADPRKQVDLYWRMGRIQY
                     GELGDADASEA"
BASE COUNT          487 a         1389 c         1440 g          677 t
ORIGIN      
        1 gcctcggagg cgtcggcgtc gccgagctct ccgtactgga tccggcccat ccgccagtac
       61 aggtcgacct gcttgcgcgg gtcggcggtc agcgtggtca ggtgcgccat catgtcgacg
      121 ccgcggtccc actcgctgat cttctcgtac agccggccga gcgcgtcgag cgcgcgctgc
      181 tcgtcgggct cgagcccgag cacgtcggcg taggcctcga tcgcgcgatc gacgtcgttg
      241 aggttctgat cgtaggtcac gcccatcgcg acgtagaggt cgacgcgggt ctggacgtcg
      301 gccgtcgcca tgatgtggtt gcggtaggtc tcgaccagcg cctcccactt gccggcctgc
      361 tggtacagcc gggcgagctc gcggtaggcg ttgtagttgc ggttgtcgag cgcgacgatc
      421 ttctcgagcg actcggcggc gcggtcgagc ttgccgaacc gctcctccca cgccgcggcc
      481 atccgctcgt acagcgcgat ctgctcggcg tccgacggcg aggcgtcgag ctgggcctcg
      541 aggacctcga ggtacttctc ggactggccg gtcttctcgt acagcgcctc gagggcgcgc
      601 agcgcggtga ggttggtcgg atcgacctgg agcaccgcct ggtaggcgtt gatcgcctgg
      661 ccggcgtcga acagccggag gtcccagatc tggccgacct cgaggcggag cttgacgatc
      721 gaggcgtcgt cggtctcgag ctcggcgcgc cgcgacatca cgtcgatcag cggctcccac
      781 tgctcggtgc ggcggtacag ccggtcgagc gcggccagcg cgccgcgcga cgccgggtcg
      841 tagaccagcg cctgctggta ggcgtggatc gcgcccgcgg cgtcctgcat ctggcgctcg
      901 agcagctcgg cgagctggat gtagagctcg gtcttcttct cgggcgacgg ctcgaccgcg
      961 gcgtggcgct gcagggtctc gatcagctcg ctccacgacc cgcgcttgcg ctggagctcg
     1021 gccatgccgc cgagcgcgcc ggtgtggctg gggtcgatcc gcagcgcctg ctgcaccgag
     1081 tggatcgcgt actcgaggtg ggacaggtgc tcggcgtacc accggccgat cttgacccac
     1141 aggtcggcgg ccgagccgcg gtcctcgtgc tcgagctcgt tgacccggtt ggtgtactcc
     1201 tcgagcagct cctgccagcg gttggtggcg gtcgcgagcc gctccagctc gtgggcggtc
     1261 tggtcgtgcg cgtagtcgcg cttgaacgcg gcctgcagca cgtagaacgc cgactcctga
     1321 tcgccgatct cggactcgta gatccgcgcg acccggttca agatcgcgag ctgctcctcg
     1381 accgacggcc ggagctcgct gcgctcgagc aggacctcga ccagctcggt ccacttgtac
     1441 tgctgggtgt agatcgcctc gaggcggtcg ctcgcggtgg cgttggacgg gtccgatgcg
     1501 cggacgcgct cgtagaccgc cgcggcgcgg gtcagctcct cgaccttctc ctcccaggtc
     1561 gcggcggcct ggagcagggt ctcgcggcgc tggacctcgt cgtcgagcac cagcgcgcgc
     1621 ttctcgagca cgtcgatcgc ctcctcccac cggccctcgc gggtgaacag cgcctcgagc
     1681 gcggcgagcg cgcggaagtc gctggggtcg atcgcgatca cgcggcgcca ggcgtcgacc
     1741 gcgtcgtcga cccggccgag cacctcgccc tcgagctggc cgagctgggc gtagagctcg
     1801 atggtctcgg actcgtcgat cgcgccggtg acctggccga cctcgatgat ccggcgcagg
     1861 gtctgcgaca gctcgtccca cgactgggtc gagcggtaca gccgggccag cgagcgcagg
     1921 gtctcgaggt cgttgccgtc gagccggtcg gcggcgagcc aggcgtcgag cgcgttgcgc
     1981 tcgcgcgcga gcttgtcctc ccacacccgg ccgagctgct tgtacagccg gatctggtcc
     2041 tggtcgccct gggtcgcggc gatctggcgc tcgatgatct cgaccagctc ctcccagcgc
     2101 tcgcgccggg tgcacagctc gccgagcgct tcgagcgcct gcgggtcctc gccgcggatg
     2161 tcgagcacgc ggttccacag ctcgatcgcc ttgtcctcgc cgaacccggg cgtgagcaac
     2221 gcgtccgacg agatccgcgc catccgggcg tagatgtcgg ccatctcgtc gtcaccgtcg
     2281 gcgacgtcga tcagcttctc gtaggtctcg tacagcctgg gccacgcctc gcggcggaag
     2341 tggatggcct cgatcgcctc cagcgccttg cggttgcggc tctcctggtc gagcaccgcg
     2401 acgtagcacg ccagggcctc gtcgaggtcg cccagcgcgt cggcgaagat cgcgccgcgc
     2461 cggaagtaca gctcgatctg ctcgtcgggg tcgcccgcga cctcgatccg gcggcgcagg
     2521 atctcggcga ggtcgtcgta catcccggcg ccgaggtaca gccggtcgag cgcggccagc
     2581 gcgccttcgt ccttgggctc gatctcgagc acgcgcaggt gggtctcgac cgcgcgcgcc
     2641 gggtcaccga tctcggtctc gtagacccgc gccatgcgca agagcgtggt gcgcttgacg
     2701 tccttgtcct tggtccgctc gagcgcgcgg gtgtaggcgg cgaccatctc gtcccacgcg
     2761 ccggtcgcgc cggcgagccg ctcggcctcc tcgaccgcgt ggtcccagcg cgggtccagg
     2821 gccaacgcct cgctccacca gtggaacgcc ttgggctggt cgtagagctt ggtctcggcg
     2881 agctcggcga ggcgctggta catcgcgctc ttgtcggcgc ccgacagctt gccgagcgtg
     2941 acctcgtgga tgccgtgcag cttgccgtac tcgccggcgg tctcgtagag cggctcgacg
     3001 acctggccga tctccatctg gaggtggccg gcgtggaaca tgtcctcgag cgcctcgagc
     3061 gtcgcgccat ggctcggctg ggcggcgagg atctcgcggt agacgtcgac cgcgccgcgc
     3121 cggtcgccga gctggttctg cagggtctgg ccgagccgga actgcagctc ggcgacctcg
     3181 ctggccgccg acgcgccgtc ggcgagctgg atctcgcggc gcaggatctc ggtgagcgcc
     3241 ggccacgccg cggtcgcgct gtacagccga tcgagcgcga gcaccgcggg cttgttgtcg
     3301 tactcgacct cgaggatccg gcggtaggtc tcgatcgccc gggcgatgtc gccgagctcc
     3361 tgctcgtgga cccgggcgag ccgggacagg aggtcgacct ggcgcggcac gtcgagcgtc
     3421 ggctcggcca ggccctcgta gagcccggcg agctggtcga agctgttgtt gatctcggcc
     3481 agccgctcga tgtggccgag cgtctgctgg ttgccggagt catcgcgcag cgcgcgcgcg
     3541 taggcgtcga acgccgcgct ggtgttgccg atcatctgct cgtgcagccg ggcgatccgg
     3601 tgcaggaggt cgacccgggc cagcgggtcg tcgttgtgcg cgaccatgac ctcgaggaca
     3661 tcgaccagct tggcgaactc gccggtggtc tcgtagatcg gctcgagcac gcgggccacc
     3721 atcaccggct cgatcttgcc gtggaccagg ccgtcgagcg cgtgcagggt ctcggcgtgc
     3781 tgcggatcga gctcgagcgc ctcgcggtag ctctcgatcg cgcgcgcgac gtcgccgagc
     3841 cggatctgcc acaggtggcc gatccggtac ttgagcgcga cggtctcgcc ggtcgcctcg
     3901 gcgagctcga cctggcgctc gaggttgccg agcaggtcgt accagcgctc ggcctggccg
     3961 tacagccggt cgagcgactg gatcgcgggc agc
//
LOCUS       DASVOR010002171         2287 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_285279,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002171 DASVOR010000000
VERSION     DASVOR010002171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285279"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VF516_40275"
     CDS             <1..1040
                     /locus_tag="VF516_40275"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8114058.1"
                     /translation="LRVAFALAAGLDGAPIRRELTRDDLARFAAPLLERCGKACRRVL
                     KDAELDKAALDGVILVGGSTRSPVVRDYVARLFERAPLCDLDPDQVVALGAAVQADVL
                     AGGQHNVTLLDVVPLSLGVEMMGGVVEKLIHRNTTIPTGATQTFTTYADNQTGFDIHV
                     VQGERETADACRSLARFVLRGVPPMIAGMGRVEITFLIDADGLLKVMAKELTTGQEAA
                     IEVVPSYGLSDEEVERMLLDSFEHAEDDLARRSLAVERVEAERILAATRQAFVGDAAL
                     LDDEVRAAGEAAMRALEAAIAGTDHLAIRAGIEALDQATKPFAQLRMNRAIAAQLQGV
                     ALDEAARKVGA"
     gene            1052..1423
                     /locus_tag="VF516_40280"
     CDS             1052..1423
                     /locus_tag="VF516_40280"
                     /inference="COORDINATES: protein motif:HMM:NF012339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEX8114059.1"
                     /translation="MPKLTFLNPHGPQSTGPLTVECKRGTSILDAAEDCGARVGHACG
                     GNLACSTCHVWVHAGLDSLPEVTDKENDIMDKAFDVRPESRLGCQARLADEDVVVEIT
                     EESLRAWLDENPEERKKARAG"
     gene            1534..2127
                     /locus_tag="VF516_40285"
     CDS             1534..2127
                     /locus_tag="VF516_40285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114060.1"
                     /translation="MGLVADSSGTGAEPTPPEALRWEFFLQQGVRPICTFVRRRQGSA
                     WGPARIVVSYPGAQPALPPDPVVPWDPVLEDWLLDHRIAARDEVNETARFGHALETRL
                     GAIERRSGSASFVAVLLRCLYDLRCELTLPLQRKLGAIRSYEPDPSSAHTAVGAELNQ
                     VLRTSQDTLAGLGYPPDRAAAIFGDALASYLRERFEL"
BASE COUNT          317 a          821 c          859 g          290 t
ORIGIN      
        1 ccctgcgcgt ggcgttcgcg ctggcggccg ggctcgacgg cgcgccgatc cggcgcgagc
       61 tgacccgcga cgacctcgcg cggttcgccg cgccgctgct cgagcgctgc ggcaaggcgt
      121 gccggcgcgt gctcaaggac gccgagctcg acaaggcggc gctcgacggc gtgatcctgg
      181 tcggcggctc gacccggtcg ccggtggtcc gcgactacgt cgcgcggctg ttcgagcgcg
      241 cgccgctgtg cgatctcgat cccgaccagg tggtcgcgct cggcgccgcg gtccaggccg
      301 acgtgctcgc cggcgggcag cacaacgtca cgctgctcga cgtcgtgccg ctgtcgctcg
      361 gcgtcgagat gatgggcggc gtggtcgaga agctgatcca ccggaacacg acgatcccga
      421 ccggagcgac ccagacgttc acgacctacg ccgacaacca gaccgggttc gacatccacg
      481 tcgtgcaggg cgagcgcgag accgcggacg cctgccgctc gctggcgcgg ttcgtgctgc
      541 gcggcgtgcc gccgatgatc gccggcatgg ggcgggtcga gatcacgttc ctgatcgacg
      601 ccgacggcct gctcaaggtg atggccaagg agctgaccac cggccaggag gccgcgatcg
      661 aggtcgtgcc gagctacggg ctgtccgacg aggaggtcga gcggatgctg cttgactcgt
      721 tcgagcacgc cgaggacgac ctcgcgcgcc gcagcctcgc ggtcgagcgg gtcgaggccg
      781 agcgcatcct ggccgcgacc cgccaggcgt tcgtcggcga cgccgcgctg ctcgacgacg
      841 aggtccgcgc ggccggcgag gcggcgatgc gcgcgctcga ggccgcgatc gccggcaccg
      901 accacctggc gatccgcgcc gggatcgagg cgctcgacca ggcgaccaag ccgttcgccc
      961 agctgcgcat gaaccgcgcg atcgccgccc agctccaggg cgtggcgctc gacgaggccg
     1021 cgcgcaaggt cggggcctag cggagacggc gatgcccaag ctgaccttcc tcaatccaca
     1081 cggcccgcag tcgaccgggc cgctgaccgt cgagtgcaag cgcggcacgt cgatcctgga
     1141 cgccgccgag gactgcggcg cgcgggtcgg ccacgcgtgc ggcggcaacc tcgcgtgctc
     1201 gacctgtcac gtctgggtcc acgccgggct cgactcgctg cccgaggtca ccgacaagga
     1261 gaacgacatc atggacaagg cgttcgatgt ccgccccgag tcgcggctcg gctgccaggc
     1321 ccggctcgcg gacgaggacg tcgtcgtcga gatcaccgag gagagcctgc gggcgtggct
     1381 cgacgagaac cccgaggagc gcaagaaggc gcgcgcgggg tgagtgatcc tggaggggcc
     1441 cagtaaccga tccgccatcg cggaccaggc ccgccgcagc ggcttgcggc gttttccgcg
     1501 tgtcatcgcc gcgcggccgg tgtaccagag ggcatgggcc tggtcgcgga cagcagcggc
     1561 accggcgcgg aaccgacgcc gccggaggcg ctgcgctggg agttcttcct ccagcagggc
     1621 gtgcggccga tctgcacgtt cgtccgccgt cgccagggca gcgcctgggg gccggcgcgg
     1681 atcgtggtgt cctacccggg cgcccagccg gcgctgccgc cggaccccgt ggtgccgtgg
     1741 gatccggtgc tggaggactg gctgctcgac caccggatcg cggcgcgcga cgaggtcaac
     1801 gagacggcgc ggttcggcca cgcgctcgag acccggctcg gcgcgatcga gcggcggagc
     1861 ggcagcgcga gcttcgtcgc ggtgctgctg cgctgtctgt acgatctgcg gtgcgagctc
     1921 accctgccgc tgcagcgcaa gctcggcgcg atccgcagct acgagcccga tccgagcagc
     1981 gcgcacacgg ccgtcggagc ggagctgaac caggtgctgc gcacaagcca ggacacgctg
     2041 gccgggctcg gctacccgcc cgatcgcgcg gcggcgatct tcggcgacgc gctcgcgtcc
     2101 tacctgcgcg agcggttcga gctgtgaccg ccgcccggcc gaggcgcacc gcctaccagg
     2161 cgatgacgtg ggcctcggag cccggctccc agcaggtgtc gctgccgcgg tacgacacca
     2221 cgaccagcgc ggcgcggcga tcgcggtcga ggtagccgcc gccgaggaac gcgtcgttgt
     2281 ggcagac
//
LOCUS       DASVOR010002172         8876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_24602,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002172 DASVOR010000000
VERSION     DASVOR010002172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8876
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24602"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="thrS"
                     /locus_tag="VF516_40290"
     CDS             <1..966
                     /gene="thrS"
                     /locus_tag="VF516_40290"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEX8114061.1"
                     /translation="ALPEDERASFSLKPMNCPSHHLFYQMKKRSYRELPLRLHTQDVL
                     HRNEATGVLSGLTRVRQFQQDDAHIYLMESQIADEVARLTRLIARVYGAFGLEFTAKF
                     ATRPVQKIGDDTLWDKAEAALQAALEATGLPYELKPGDGAFYGPKIDFDVADSIGRKW
                     QLGTIQLDYNAPERFDLTYIGSDNTEHRPVVIHRAIYGSFERFIGILIEHYAGNFPVW
                     LAPEQARILPVAERHHAWADEVAARMRAADLRVDIDRSHGKLGAMIRDAQLAKIPYAL
                     VVGDKEVAAQAVSPRKHGTGKEADLGLMPLDSFLAQIGKESEIPY"
     gene            complement(1006..1638)
                     /locus_tag="VF516_40295"
     CDS             complement(1006..1638)
                     /locus_tag="VF516_40295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114062.1"
                     /translation="MNKRLTHVSLARTRLAMFTWAAVVAACSSPAPGAERAGRAIRHN
                     QEDQMTCETELKRLDSGDWRGWSGLPAGCGPQQLAAVFQGGDTVIPGRLSGQPTPLRI
                     YRSVNQKEEIHAWFDDAHVFLLTLDSPTTDMAADSILQAWGSPEKKLHPLVGMHADAP
                     QWIYASRGFTVFVRERDGGIARVAAYRPSSVEYYEQYLGATDKKHYVPRR"
     gene            complement(1638..5150)
                     /locus_tag="VF516_40300"
     CDS             complement(1638..5150)
                     /locus_tag="VF516_40300"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8114063.1"
                     /translation="MTRWMHPPELARTPERDQFDPVIDGKRYGLPRELLLAIWERVCR
                     DATDRAGRLDADPMQRRFHELAAWIAAHDGRLHSDVGRLTYVQTDTIGVPRGALAVDE
                     LAPRAPGRTTLVDAETRPRTMSGWLPPHPLLPMDVAKPLAPGRQTLVGHVPSPRLGFE
                     DYRVRGLQGVLQRLGPEHSLRAELIAAAASTDRSTAGRATLWREPLPVTATPEGHALW
                     RVAERHAAMLYRRAVHDGVVEEHDPAIESALHQRGTGQSLPAELRRDMERALGVSLAR
                     VRIHTDMVAAHAARVLGAEAFTVGEDIFFADGAFAPEADRGIKLLAHELAHVIQGWQG
                     RAAVGQQRSRVSDPRDPLEHEADAIAERVDQRLAGEKASLHPHVAATGQPDRGLRVAG
                     PIQAAGLENSPEAAAHLPAHGDGAKLPEDVRRKMEAVFGVDFSSVRIHEGAYVEAVGA
                     LAYTQGTHIHFAPGQYQPTSQRGQELLGHELAHVVQQSQARVRTTIHAGGVEINDDAS
                     LEREADEMGARAAQAEVGREPLRRPAGGWLDDAASQQNHQEPPWSGAGAMDLASALDH
                     PSRGHATRSANAASPRAGVIQRQHVRLPSGRYVGDLPGSTTNVREDVLRVMDKLHEMW
                     SLSNPDYMAEYPVVGGQPVQSHIPAAMIPRTIAALQANEQPTLAPGPAQQILGITISM
                     PVGRGQPNNQVDIYRLQDALHSLWHISNSDYATERAAVNAGPDPVPEGIIPRTIDGIA
                     QMKVAYVSGRWRGTGGLLAGTAPVSASQQGRIRPNLTPGVPPGTTFRDLDGGRSYRDH
                     LWEMLELLRAQWYTESRDLLAATPAPMSRFEEIAAEAKQQVDADFGQYVTGPALTAAA
                     GNLVDLSTFPPSAPDLIEYLINEDDHSQPIRTRHGADNARSARPAYTVPAAGVTTVAQ
                     VAAANHTTPQAIASANGLTATSTVSPGQILNLPFEPDIVAQVITDYCSDPTRQSQYEV
                     IDRAWPGAEIGGRVGIQRAVGSAEGPRRRAFWENFQTVIHEYLHSITHPNYTRVAESM
                     GQVQKGVLIEGGTSLFTDHVWQRLNPTVIRSNAALRQHVEGRAHTYQAGVIPPISHYG
                     TIAQARNIEGIVGPANMRAAYFLGNTELLGFTGPGTYTVPPHGVTTVADVAYMTGLSA
                     EDIAALNSLRPSSNVTPGQSLRLR"
     gene            5590..6027
                     /locus_tag="VF516_40305"
     CDS             5590..6027
                     /locus_tag="VF516_40305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002959.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEX8114064.1"
                     /translation="MTFVIRPFASTDETDVVALWQAAGLTRPWNDPHADIRRKQAVQP
                     ELFLIGEFNEEIVASAMAGYDGHRGSVYYLAVSPAHQRCGFGRAMMAEVERRLEALGC
                     PKLNLMVRHDNRVAIGFYKRLGYDEAAVVTLGRRLTADAPQAT"
     gene            complement(6200..7492)
                     /locus_tag="VF516_40310"
     CDS             complement(6200..7492)
                     /locus_tag="VF516_40310"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HEX8114065.1"
                     /translation="MAIWNPDVETLPRGRMEALQLDRLRACVARVLATVPPLASRLRD
                     AGLGDARDLRSLADLRRLPFTTKTDLRDHYPFGLLGVPRDQVVRIHASSGTRGKPTVA
                     AYTRNDLAVWSEVVARCLVMAGARAGMVVHNAYGYGLFTGGLGFHQGAELLGCTVLPI
                     SGGFTQRQVMLLQDFGGAVLCATPSYALNIAEALERASIDPRELRLELGMFGAEPWTE
                     QMRGEIERRLGLTAINVYGLSEIVGPGVSCECREVRDGAHIHEDHFLPEIIDPDTGEP
                     LPPGREGELVFTPVTKEALPLIRYRTGDIATLDAAPCACGRTTVRMSRVRGRRDDMLI
                     IRGVNLYPSEVERIVLGVPDLAPHYQLIVERPGALDELILVCEPARDGVDREALRARL
                     SHELHQQTGITIAVRIVDCDQVPRSEGKAVRVVDHRPR"
     gene            7549..8094
                     /locus_tag="VF516_40315"
     CDS             7549..8094
                     /locus_tag="VF516_40315"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX8114066.1"
                     /translation="MKLGKRWIAQIPPSEEPDVWVPMVNEIPAGSSCKYRLDKTTGQL
                     ALARVLPHEVTYPANYGFVPQTRGADDEEVDILVLAREPLLPLTIVRAHVIGGFVERT
                     SDQPEPEERLLATAIDDPSVAGVHGVDDLGELRDRIEAFVRTYKQDQGVEVTFEGWID
                     RDAALDRLRRGFKRAKKRPAK"
     gene            8289..8579
                     /locus_tag="VF516_40320"
     CDS             8289..8579
                     /locus_tag="VF516_40320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114067.1"
                     /translation="MRYCVTRIERELGEIEHWSVTIAPAGGEFTSIVAIKDGCEAIES
                     TGTGMDGPLAAWEALCRLEQLLREARAHRTADAALATGTPVRRHLVLVDGAH"
BASE COUNT         1435 a         3011 c         2959 g         1471 t
ORIGIN      
        1 gcgctgcccg aggacgagcg cgcgagcttc tcgctcaagc cgatgaactg cccgtcgcac
       61 cacctgttct atcagatgaa gaagcgcagc tatcgcgagc tgccgctgcg gctgcacacc
      121 caggacgtgc tgcaccgcaa cgaggcgacc ggcgtgctga gcgggctgac ccgggtccgc
      181 cagttccagc aggacgacgc gcacatctac ctgatggagt cgcagatcgc cgacgaggtc
      241 gcgcggctga cccggctgat cgcccgggtc tacggcgcgt tcggcctcga gttcaccgcg
      301 aagttcgcga cgcgcccggt gcagaagatc ggcgacgaca cgctgtggga caaggccgag
      361 gccgcgctcc aggccgcgct cgaggccacc ggcctgccct acgagctcaa gcccggcgac
      421 ggcgcgttct acggcccgaa gatcgacttc gacgtcgccg acagcatcgg ccgcaagtgg
      481 cagctcggca cgatccagct cgactacaac gcacccgagc ggttcgatct gacctacatc
      541 ggctcggaca acaccgagca ccggccggtc gtgatccacc gcgcgatcta cggctcgttc
      601 gagcgcttca tcggcatcct gatcgagcac tacgccggca acttcccggt ctggctcgcg
      661 cccgagcagg cgcggatcct gccggtcgcc gagcgccacc acgcgtgggc cgacgaggtc
      721 gccgcccgga tgcgcgccgc cgacctgcgg gtcgacatcg atcgctcgca cggcaagctc
      781 ggcgcgatga tccgcgacgc ccagctcgcg aagatcccct acgcgctggt ggtcggcgac
      841 aaggaggtcg ccgcccaggc ggtgtcgccg cgcaagcacg gcaccggcaa ggaggccgac
      901 ctcggcctga tgccgctcga cagcttcctg gcccagatcg gcaaagagtc cgagattccc
      961 tactaggaat gccatgaaca ccaaggacgc gtccgaatcc ggccatcacc gacgcggcac
     1021 gtagtgcttc ttgtcggtgg cgcccaggta ttgttcgtaa tactcgacgg agctcggtct
     1081 gtacgctgcc acgcgagcga tgccgccatc tcgttcgcgc acgaacacgg tgaagccgcg
     1141 actcgcatag atccactggg gggcgtcggc atgcattccc acgagcggat gcagtttctt
     1201 ttcgggagat ccccacgcct ggaggatgga atcggcggcc atgtctgtcg tcggcgaatc
     1261 gagcgtgagc aagaacacgt gagcatcgtc gaaccaggcg tggatctctt ccttctggtt
     1321 gacgctgcgg tagatgcgta gcggggtcgg ctgtcccgag agccggcctg gaatcaccgt
     1381 gtccccgcct tgaaagacgg cggcgagttg ctggggtccg catccggccg gtagccccga
     1441 ccagcctcgc cagtctccgc tgtcaagacg cttcagctcg gtctcgcagg tcatctggtc
     1501 ctcctggttg tgccggatgg cccggcctgc gcgctcagcg cctggtgccg gcgagctgca
     1561 tgcggcaact accgcggccc atgtgaacat ggccagcctg gtgcgagcca gggatacatg
     1621 ggtcagacgc ttgttcatca tcgcaatcta agggattgac cgggcgtcac gtttgacgac
     1681 ggtcgcagcg aattgagcgc cgcgatgtcc tcggcgctca gacctgtcat gtaggcgaca
     1741 tcggccacgg tcgttacccc gtgtggcggc acggtgtatg tgccaggccc ggtaaaaccc
     1801 aggagctcgg tgtttccaag aaaatacgcc gcccgcatgt tggccggccc gacgataccc
     1861 tcgatgttcc gtgcctgagc gatggtgcca tagtggctga tgggcggaat gacgccagct
     1921 tggtatgtat gagcgcgacc ctcgacatgc tgtcgcagtg ccgcgttgct acggatcact
     1981 gtcgggttga gacgttgcca gacgtggtcc gtgaacaagc tcgtcccacc ttcgatgaga
     2041 acgccctttt gtacctggcc catgctttca gctacacgtg tgtagttggg atgtgtgatc
     2101 gagtgaaggt attcgtgaat cacagtttga aagttctccc agaaagctcg tctgcgcggc
     2161 ccctcggcac tccccaccgc gcgctgaata cccacacgac caccgatctc agcaccgggc
     2221 caggcgcggt cgatcacctc gtactggctc tgtctcgtcg gatcggagca gtagtcggtg
     2281 atgacttgag cgacgatgtc tggctcgaac ggcaagttga gtatctggcc tgggctcacg
     2341 gtcgaggtcg cagtcagacc attcgcactc gcgatggcct gcggcgtggt atgattggct
     2401 gctgcgacct gggcaaccgt ggtcacgccc gccgccggca ccgtgtacgc tggtcgcgcc
     2461 gaccgtgcat tgtctgcacc atgccgcgtt cgtatgggtt gcgaatgatc gtcttcgttg
     2521 atgagatatt caatcaggtc gggtgcgctg ggtgggaacg tcgacaggtc aaccagattc
     2581 cccgcggccg ccgtgagcgc tggacccgtg acgtactgtc cgaaatcggc gtcgacctgc
     2641 tgcttggctt cggctgcaat ctcctcgaac cgcgacatcg gcgccggtgt tgcggccaac
     2701 aaatcccgcg attccgtata ccactgtgcg cggagaagct cgagcatctc ccacaagtga
     2761 tcccggtacg agcggccccc gtccaggtcc cgaaatgtgg tccccggcgg tacgcccggg
     2821 gtcaggttgg gccgtatcct cccctgctgg ctggcgctga ccggcgcggt tcctgccagc
     2881 aacccgcccg tgccgcgcca tcgccccgat acgtaggcaa ccttcatctg cgcgatgccg
     2941 tcgatcgttc tcggaatgat cccctcgggg accggatccg gtccggcatt gactgctgca
     3001 cgctcggtag cataatcgga attgctgatg tgccacaggc tgtggagcgc atcctggagc
     3061 cgatagatat cgacctgatt gttgggctgg ccgcggccca cgggcatcga gatcgtgata
     3121 ccgagaatct gttgcgccgg cccgggggcc agagtcggct gctcgttggc ttgcagcgcg
     3181 gcgatggttc gcgggatcat cgccgcggga atgtggcttt gcaccggttg tccaccgacc
     3241 acggggtatt cggccatgta atccgggttg ctgagactcc acatctcgtg gagtttgtcc
     3301 atcacccgta agacgtcttc gcggacattg gtcgtactcc ccggcaggtc gccgacatac
     3361 cgccccgatg ggagtcgaac gtgctgtcgt tgaatgacgc ccgcacgcgg cgatgccgca
     3421 tttgccgaac gggtcgcgtg gcctcgtgag gggtggtcca gggcggaggc caggtccatc
     3481 gccccggcac cggaccatgg aggttcttga tgattctgct gactggccgc gtcatccagc
     3541 caccctccgg ccggcctacg cagcggttct cggcccactt cggcctgcgc tgcccgcgcg
     3601 cccatctcgt ccgcctcgcg ctccagcgat gcgtcgtcat tgatctccac gccgccggcg
     3661 tggatcgtcg ttcgcacccg tgcttgcgac tgctgcacca cgtgcgcaag ctcgtgcccc
     3721 agcagttcct gtccacgctg gctcgtgggc tgatactgcc cgggggcaaa atggatatgc
     3781 gtcccctgcg tgtacgccag cgccccgacc gcttcgacgt acgctccctc atggatgcgc
     3841 accgacgaaa aatccactcc aaacacagct tccatcttgc gtcgcacatc ctccggcagc
     3901 tttgctccgt cgccatgagc gggtagatgt gctgccgcct cggggctgtt ctccagtcct
     3961 gcagcctgga tggggccggc cacgcgcaga cctctgtccg gctgtccagt cgctgccacg
     4021 tgcgggtgga gggatgcttt ctcaccagcc agccgctggt cgacccgctc cgcaatcgcg
     4081 tcggcttcat gctcgagcgg gtccctcgga tcactgaccc ggcttctctg ttgtccgacg
     4141 gcggccctgc cctgccagcc ctggatgacg tgcgcaagct cgtgggcgag cagcttgata
     4201 cctcgatcgg cctccggggc gaaggcacca tcagcgaaaa agatatcctc gcccaccgtg
     4261 aacgcctcgg caccgagtac acgcgcggcg tgggctgcga ccatgtccgt gtggatgcga
     4321 acgcgagcaa gcgagacgcc gagcgcgcgc tccatatcgc ggcgcagttc cgctggaagc
     4381 gactggccgg taccgcgctg gtggagagcg gactcgatcg caggatcatg ctcttcgacg
     4441 acgccatcgt gcaccgcgcg gcgatacaac atcgcggcat ggcgctccgc gacccgccac
     4501 agagcatggc cctccggagt cgcggtgaca ggcaacggct cccgccacag tgtcgcgcgc
     4561 cccgcggtcg agcgatctgt gctcgcagcg gccgcaatga gctccgctct cagcgagtgc
     4621 tccggcccta gccgctgcag aactccctgg agcccgcgta cccggtagtc ctcgaagcca
     4681 agccgaggac tcggcacgtg gccgaccagt gtctgtcgcc ctggggcgag cggcttcgcg
     4741 acatccatgg gcagcaacgg gtgaggaggc agccagccgc tcatcgtccg cggccgcgtc
     4801 tcagcatcga ccagcgtcgt ccggcccggg gcgcgcggag ccagctcgtc gacagccaat
     4861 gcaccacgcg ggacgccgat ggtatcggtc tgcacgtacg tgagccggcc tacgtcggag
     4921 tgcaaccgtc cgtcgtgcgc cgcgatccac gcggccagct cgtgaaatcg tcgctgcatc
     4981 gggtccgcat cgagtcgacc cgcacggtcg gtcgcatccc tgcatacccg ctcccagatc
     5041 gcgagcagta gctcccgggg cagtccatag cgtttgccgt cgatgaccgg gtcaaactga
     5101 tctcgctccg gcgtacgagc gagctcgggt ggatgcatcc agcgcgtcat cgcgtgctcc
     5161 ctcctgcctc acgtcacctg ctccctcgtc gtatctcaag tcgactgttc ttggctatgc
     5221 ctttctttgt ggggagcttc atcaccaatc cgaccgatca ctctcggcct gcagctcgag
     5281 agttcctttg tcgcgtacag tgtctctgca aatccggtcg acccacagac gttgcgaggt
     5341 agagcgatat gcgccgagac caagaagcgg cctttcttgt acccgccggc tctgggcttg
     5401 ccgcgggggt gagcaatagc ggcgtgttcg cgatgcgggt caggaacgca agtgtaagga
     5461 atcataccgc aggcagcgca agggcagacc atgtcatgcc ggcgggcgtc ggcacggtga
     5521 gttccgggat cgatggcctg gcattccatg ccacgcgggg tcgtccccgg cggcatcgta
     5581 tcatgccgca tgacattcgt gattcgcccg ttcgcgagca ccgacgaaac cgacgtcgtc
     5641 gcgctgtggc aggccgcggg gttgacccgg ccatggaacg atccacatgc cgatatccgc
     5701 cgtaagcagg cggtgcaacc cgagctgttt ctgatcggtg agttcaacga agagatcgtc
     5761 gccagcgcga tggcgggcta cgacggacac cgcggcagcg tctactacct cgcggtgagc
     5821 cctgcccatc agcgctgcgg attcggccgg gccatgatgg ccgaggtcga gcgccggctc
     5881 gaggcactcg gttgcccaaa gctcaacctg atggtacgcc acgacaaccg cgtcgcgatc
     5941 ggcttctaca agcgcctcgg gtacgacgag gcagcagtcg tgaccctcgg cagacggttg
     6001 accgctgatg cgcctcaggc gacgtgatct ggcggctagc agcagagcta cgactgcggc
     6061 atggaggtgg tgttgtgtat gcggcaggtt gccggcgatc gtcgacacca tgttggtctg
     6121 aagcccgcgc gaaatctcat gaccgcgatg cgacactgtc ggataccatc gagcggatgg
     6181 gcaaatacat gggttcaggt caccgcgggc gatggtcgac cacacgcacc gccttgccct
     6241 cgctgcgcgg cacctgatcg cagtcgacga tgcggaccgc gatcgtgatg ccggtctgct
     6301 ggtggagctc gtgggacagc cgcgcgcgca gcgcctcgcg gtcgacgccg tcgcgcgccg
     6361 gctcgcacac caggatcagc tcgtcgagcg cgcccggccg ctccacgatc agctggtagt
     6421 gcggcgccag gtcgggcacg ccgagcacga tgcgctcgac ctccgacggg tagaggttga
     6481 cgccgcggat gatcagcatg tcgtcgcgcc ggccgcggac ccggctcatc cgcaccgtcg
     6541 tgcggccgca cgcgcacggc gccgcgtcga gcgtcgcgat gtcgccggtg cgataccgga
     6601 tcagcggcag cgcctccttg gtgaccggcg tgaacaccag ctcgccctcg cggcccggcg
     6661 gcagcggctc gccggtgtcc ggatcgatga tctcgggcag gaagtggtcc tcgtggatgt
     6721 gcgcgccgtc gcggacctcg cggcactcgc acgacacgcc ggggccgacg atctcggaca
     6781 ggccgtagac gttgatcgcg gtcagaccga gccggcgctc gatctcgccg cgcatctgct
     6841 cggtccacgg ctcggcgccg aacatgccga gctcgaggcg gagctcgcgc gggtcgatcg
     6901 acgcccgctc cagggcctcg gcgatgttga gcgcgtacga cggcgtcgcg cacagcacgg
     6961 cgccgccgaa gtcctggagc agcatcacct ggcgctgcgt gaacccgccg gagatcggga
     7021 gcacggtgca accgagcagc tcggcgccct ggtgaaagcc gaggccgccg gtgaacaggc
     7081 cgtagccgta ggcgttgtgc acgaccatcc cggcgcgcgc gccggccatc accagacagc
     7141 gcgccacgac ctcgctccac accgcgagat cgttgcgggt gtacgcggcc acggtgggct
     7201 tgccgcgcgt gccgctggag gcgtggatcc ggaccacctg gtcgcgcggc acgccgagca
     7261 agccgaaagg gtagtggtcg cgcagatcgg tcttcgtcgt gaatggcagc cgccggaggt
     7321 cggccagcga gcgcaggtcc cgcgcatcgc cgagccccgc gtcgcgcagc cgggaggcga
     7381 gcggagggac cgtggcgagc acgcgcgcca cgcacgcgcg cagtcgatcg agctggagcg
     7441 cctccatccg gccgcgcggc agggtctcca cgtcgggatt ccagattgcc atcgcggcga
     7501 tactaacctt cccgccggac gagcacactg cttgcaacgc accgggccat gaagctaggc
     7561 aagcgctgga tcgcgcagat tccgccgtcc gaggagcccg acgtctgggt gccgatggtc
     7621 aacgagatcc cggcggggtc gtcctgcaag taccggctgg acaagaccac cggccagctc
     7681 gcgctggcac gcgtgctacc gcacgaggtg acctatcccg ccaactacgg gttcgtgccc
     7741 cagacccgcg gcgccgatga cgaggaggtc gacatcctgg tgctggcgag ggagccgctg
     7801 ctgccgctga cgatcgtgcg cgcgcacgtg atcggcggct tcgtcgagcg caccagcgat
     7861 cagcccgagc ccgaggagcg gctgctcgcc accgcgatcg acgatccgag cgtcgcgggc
     7921 gtgcacggcg tcgacgacct cggcgagctt cgcgaccgga tcgaggcgtt cgtccggacc
     7981 tacaagcaag atcagggcgt cgaggtcacg ttcgagggct ggatcgaccg cgacgcggcg
     8041 ctcgaccggc tgcgccgcgg cttcaagcgc gcgaagaagc ggccggccaa gtgaccgccg
     8101 ccggtccgga ttgccgtcga cgacggcctc cgccggcacg ggagcgcgtg gcaccggggt
     8161 tgcttgcttt gcccgcggag gccgacatgg aatccaggtg cgccagagcg cgcgcaatgc
     8221 gtgcgcgggt cgagctgcaa cttcaccgcg gagcgggggc tcgccagtac cgagagctcg
     8281 gggccttcgt gcgctactgc gtgacccgga tcgagcgcga gctcggcgag atcgagcact
     8341 ggtcggtgac gatcgcgccc gcgggcggcg aattcaccag catcgtcgcc atcaaggatg
     8401 gctgcgaggc gatcgagtcg accggcacgg gcatggacgg gccgctcgcc gcctgggagg
     8461 cgctgtgccg gctcgagcag ctgctgcgag aagcccgggc ccaccgcacc gccgacgcgg
     8521 cgctcgccac gggcaccccg gtccggcggc acctcgtcct cgtcgacggc gcacattgag
     8581 ccacccgcgc ccgggcgccg aagccagctg tgacgcaggt ctcgacggct tcgagggttg
     8641 cccgggcacc gagggttgat cctcgccgga tggcgagtcg gtgtgcctgc gcagcacgcg
     8701 gcggggcgca caccgtgcgc ccttgcgccc ctggacggca gaacggccgg ccggcgaacg
     8761 gcggtgcccc ggatgatgcc gcactgcgcc gacgtcgact gactgcccct ggccgaggag
     8821 gcaaccggat cgggccgcgg cgccaacggc cgaacgacgg tggccgaacg acggga
//
LOCUS       DASVOR010002173         5303 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_140179,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002173 DASVOR010000000
VERSION     DASVOR010002173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5303
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140179"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VF516_40325"
     CDS             <1..489
                     /locus_tag="VF516_40325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114068.1"
                     /translation="CLLLAFACLLAACPSAGGTYVQNGRQEGEVDRGETNGRRFDFVS
                     NKPDLDEWVIRVTGSSMWVSFAQEESTDKLGSINLTDKETRKLWKLIDAVDIPHRKKG
                     KKDEDEGFVALQLKEPGEDGGEVRRIYISRATANKDEDLKALAVYLQDLTEKYKKERP
                     SF"
     gene            1013..1420
                     /locus_tag="VF516_40330"
     CDS             1013..1420
                     /locus_tag="VF516_40330"
                     /inference="COORDINATES: protein motif:HMM:NF017451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEX8114069.1"
                     /translation="MPALVTGHALRGASGDDAPDAGAIHTEVPMFDALEMSLSVLEQL
                     APLETKLRQRRKSLADEVCRAAESVALNISEARQRAGLDRADLFRRAAGSASELTTAL
                     RIARARGYITAADHTAVDAALDRIRAMLWRLTH"
     gene            2239..2868
                     /locus_tag="VF516_40335"
     CDS             2239..2868
                     /locus_tag="VF516_40335"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8114070.1"
                     /translation="MNKHRILFLAANPSKSSGVSLDQECAAIERELRMTTHRDDFDFR
                     SKWAVTVDEMMRHLGELQPTIIHFSGHGANPAGSPVNPTGKSGIFLLDDRGGPQLVTT
                     RALMMMIKSASASTRIVLLNACYSEPQADAVRSVVDCVVGMAGAIDDDAARSFAVGFY
                     RALGNRRSVGNAVDQAVATLAAKGMPDEKLPRCLTRDGVDAYKVTLDPS"
     gene            complement(2882..4312)
                     /locus_tag="VF516_40340"
     CDS             complement(2882..4312)
                     /locus_tag="VF516_40340"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8114071.1"
                     /translation="MFYLYLVYGAVFFATGVLLGFQARLPSVVPSRALWWLSAAMMIH
                     GVSEWMMMSAHVLGRLDAAWLWPGSLALRGVSFVLLAQFAIVLLGSRKRWPQLIPVLI
                     LAGWIGTLVTVAIAIDLDDPSIRILEAVSRYALGIPAALLTARAFWVLHRERGQRDIV
                     GGYLRWAGVAFAVYAVVAGVIVAPAAFFPASRINASAFIGLTGIPVEVIRLACGVAIA
                     FALSEALVIEVARQRHELERRREEFISVVAHDLRSPLNSIHLSAELLEARLGELGEDI
                     DAKHHQHATQLLHNIRTGAGGLERMIGDLLDASRIETRMLALEARDVDVRSLVGGIVD
                     RASKATAGHTVKLVVPELLPKIHVDAMRVEQVLVNLLSNAAKYSTPSTEIVVEAMQHR
                     DVVELAVTNRGSGLSADETAQVFSRFYRSKQHVGRAEGLGLGLYIAKGIVDAHGGRIW
                     VDSEPGRYASFRFTLPRADAPAIARS"
     gene            complement(4437..5111)
                     /locus_tag="VF516_40345"
     CDS             complement(4437..5111)
                     /locus_tag="VF516_40345"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114072.1"
                     /translation="MNVVVTGANRGIGLELVRQLLARGDQVEAACRRPDDARELRATG
                     ARLHAVDVADGANVATFADALGDLPIDLVINNAGVFGDMRQRLADFNYESATRTFEVN
                     ALGALRVSQALLPHLRRGTGKKLAYISSVLGSISNTTAPDHLAYRVSKAALNMIARSI
                     AFELRDDRILSIVVHPGWVRTAMGGPDAPTSPAEAAEAILAQIDAATFTETGEFLDTQ
                     GARCAW"
BASE COUNT          846 a         1903 c         1781 g          773 t
ORIGIN      
        1 tgcttgctcc tcgcgttcgc gtgcctcctc gccgcgtgcc cgtcggccgg cggcacctac
       61 gtccagaatg gccgtcagga gggcgaggtc gaccgcggcg agaccaacgg ccgccggttc
      121 gacttcgtgt cgaacaagcc cgatctcgac gagtgggtga tccgcgtcac cggcagctcg
      181 atgtgggtca gcttcgcgca ggaggagtcc accgacaagc tgggctcgat caacctgacc
      241 gacaaggaga cccgcaagct gtggaagctg atcgacgccg tcgacatccc gcaccgcaag
      301 aagggcaaga aggatgaaga cgaggggttc gtcgcgctgc agctcaagga gcccggagag
      361 gacggcggcg aggttcgccg gatctacatc tcgcgcgcga ccgcgaacaa ggacgaggac
      421 ctgaaagcgc tggcggtgta cctgcaggat ctgaccgaga agtacaagaa ggagcgcccg
      481 agcttctgac cggcgccctc acgtgggggc atagccgcga ccgcagccgc agccggcacc
      541 gcttcggcga ccgctgccgc aaccgcttcc gcgaccgcca ccgcttcggc gaccgctgcc
      601 gcaaccgctt ccgcgaccgc caccgcttct gcgaccgctg ccgcaaccgc ttccgcgacc
      661 gccaccgctt ctgcgaccgc tgccgcagcc gcttccgcat cggcctccgc ttccgcgacc
      721 gcaaccggtt ccgcctccgc ttccgcgacc gcagccgctt ccgcatcggc ctccgcttcc
      781 gcgaccgcag ccgcttcggc ggtgagcgtg cactgcggac atggtctccg cgcgcgagtg
      841 ctcccacgct gcgccgctcg gtgcactcac agcggcctcc gcgatcgccc tggcaccggg
      901 ggcgtcgccc ttgcgccgct ctcgcgcacc gtcccttgcc gagcgccgga ccgaccgacg
      961 tgacgcgtgc ggcgccccca cagctggatc catcgacgag ccgcggatcg ctgtgcccgc
     1021 gctcgtcacg ggacatgcgc tccgcggcgc atcgggcgac gacgcccccg atgccggggc
     1081 gattcacacg gaggttccga tgtttgatgc actcgagatg tcgctcagcg tgctggagca
     1141 actcgctccc ctggagacca agctccgcca gcgccgcaag tcgctcgccg acgaggtctg
     1201 ccgcgcggcc gaatcggtcg ccctcaacat cagcgaagcg cggcagcgcg ccggccttga
     1261 ccgcgccgac ctgttccgcc gcgccgccgg ctcggccagc gagctcacca cggcgcttcg
     1321 catcgcacgc gcacgcggct acatcaccgc ggccgatcac actgcggtcg atgccgcgct
     1381 ggatcggatc cgcgcgatgc tgtggcggct tacgcactga gctccctcgg gccatctggc
     1441 ccccgccatg aagctgcggt agcccaaacg cgagcgccag cggtcggccc cgtgacgccc
     1501 cggtcccgcc cacccccacg tggggggcaa gctgctggtc cggcaccgaa ggttgcgaag
     1561 tggcgccgtt gcgccaacac gctcatcgct gggcttggcc acgggccagc tcgcgcgatt
     1621 ccgcggaatc gccgggctcg gatcgtgagg actgcggcgg ctgtgcttgc ccagcgccgc
     1681 gatctcggag cgtcgtgccc agggtcctga gaacgattcc gcggaatcgc cctacttctg
     1741 accgccgcgt ctgctgggac acgctcccag ccgccacgac catggtgccg agaacgattc
     1801 cggggattct cccgggtccg cggccaccag ggagtcagcg cccgaacggg ccggccacat
     1861 gggtgagatc ctgaccggcg tcctcgttgt gccggccagc attggccgaa gcggtagtgg
     1921 gcagcggttc cggcaccggc agtggttccg gcaccggcag cggccgcgga tggagatgcg
     1981 gtcgcggttc cggccgcggt ctcgtggcga cccctccgca gccagcgcta cgaaggatcg
     2041 cccgcaacat ggtgggcaag cagtcgatag aaccagcaga cgccgcaatt cggcggatcg
     2101 ctgtcgtgat cacagcggat cggtcgccta ccgggagatg acaccaggtg actcatctac
     2161 gatgacgcat ctcgctgcag cggcgagcac acccgcgcca tgtcgtgcgc tgccacggcg
     2221 acggggcggc gggatgccat gaacaagcac cggatcctgt tcctcgccgc gaacccgagc
     2281 aagagctcgg gggtctcgct cgaccaggag tgcgccgcca tcgagcgcga gctccgcatg
     2341 accacgcatc gcgacgactt cgacttccgc tcgaagtggg ccgtgaccgt cgacgagatg
     2401 atgcgccacc tcggcgagct ccagccgacc atcatccact tcagcggcca tggcgctaac
     2461 cccgcgggct cgcccgtcaa cccgacaggc aagagcggga tctttctcct ggacgatcgc
     2521 ggcggtccgc agctcgtgac cacgcgtgcc ctgatgatga tgatcaagtc cgcctcggcc
     2581 tcgacacgca tcgtgctgct caacgcctgc tacagcgaac cacaggcgga cgcggttcgc
     2641 agcgtggtcg actgcgtggt cggcatggcc ggtgcgatcg acgatgacgc ggcgcgctcg
     2701 ttcgcggtcg gcttctaccg cgcactcggc aaccgccgct cggtcggcaa cgcggtcgac
     2761 caggccgtgg cgaccctcgc cgccaagggc atgcccgacg agaagttgcc gcgctgcctg
     2821 acccgcgacg gcgtcgatgc atacaaggtg accctggacc cttcgtagcc ccgtccggcg
     2881 actacgaccg ggcgatcgcc ggcgcgtcgg cgcgcggcag cgtgaagcgg aacgaggcgt
     2941 agcggccggg ctcggagtcg acccagatgc gaccgccgtg ggcgtcgacg atccccttgg
     3001 cgatgtacag cccgagcccg agcccctcgg cccgcccgac gtgctgcttg ctgcggtaga
     3061 atcgcgagaa cacctgggcg gtctcgtcgg cggacagccc gctgccgcgg ttggtcaccg
     3121 cgagctcgac cacatcgcga tgctgcatgg cctcgacgac gatctcggtg ctcggggtcg
     3181 agtacttggc ggcattcgac agcaggttca cgaggacctg ctcgacccgc atcgcatcga
     3241 cgtggatctt cggcaggagc tccggcacga ccagcttcac cgtgtgtccg gccgtcgcct
     3301 tgctcgcgcg gtcgacgatc ccaccgacca gcgatcggac gtcgacgtcc cgggcctcga
     3361 gcgcgagcat ccgggtctcg atgcgcgacg cgtcgagcag gtcgccgatc atgcgttcga
     3421 gcccgccggc gccggtccgg atgttgtgca gcagctgggt cgcatgctgg tggtgcttcg
     3481 cgtcgatgtc ctcaccgagc tcgccgagcc gcgcctcgag cagctcggcg ctcaggtgaa
     3541 tgctgttgag cgggctccgg agatcatggg cgaccacgga gatgaactcc tcgcggcggc
     3601 gctccagctc gtggcgctgc cgcgcgacct cgatcacgag ggcttcgctg agcgcgaacg
     3661 cgatcgccac cccgcatgcc aggcgaatta cctcgacggg aatgcccgtg aggccgatga
     3721 acgcgctcgc gttgatgcga gaagccggga agaacgccgc gggagccacg atgacgccgg
     3781 cgacgaccgc gtacacggcg aacgccacgc ccgcccaccg gaggtaaccg ccgacgatgt
     3841 cgcgctgacc tcgctcacga tgcaacaccc agaacgcgcg cgccgtgagc aatgccgccg
     3901 gaatgcccag cgcgtaccgg ctcaccgcct cgagtatgcg gatggacggg tcgtccagat
     3961 cgatcgcgat ggcgaccgtc acgagggtac cgatccaccc ggccaggatc agcaccggga
     4021 tgagctgcgg ccaccgcttg cgggatccga gcaggacgat cgcgaactgc gcgagcagca
     4081 cgaacgacac gccgcgcagc gccagcgaac cgggccacag ccacgcggca tcgagccgac
     4141 cgagcacgtg ggcggacatc atcatccact ccgacacgcc gtggatcatc atcgccgcgg
     4201 acagccacca cagcgcgcgg ctcggcacga cgctcggcaa gcgggcctgg aagccgagca
     4261 agacccccgt cgcgaagaac acggcgccgt agaccaggta gagatagaac atgtctctcg
     4321 ctgatcgatg cttgccacgc ggaacatcgc gttcctacgg cgatccggtg cgtgcgggcg
     4381 gcgctcaggc gagtggttct cgcagcccag acgcggtccg gagttccagg tgcctgttac
     4441 caggcgcagc gtgcgccctg cgtgtcgagg aactcgccgg tctcggtgaa cgtcgcggcg
     4501 tcgatctggg cgaggatcgc ctcggcggcc tcggccggcg aggtcggcgc gtcggggccg
     4561 cccatcgcgg tgcgcaccca gccggggtga accacgatcg acaggatccg atcgtcgcgc
     4621 agttcgaacg cgatcgaccg cgcgatcatg ttgagcgctg ccttggacac ccggtacgcg
     4681 aggtggtccg gcgcggtcgt gttgctgatc gagcccagca ccgacgagat gtacgcgagc
     4741 ttcttgccgg tgccgcggcg caggtgcggc agcagcgcct ggctgacccg cagcgcgccc
     4801 agcgcgttga cctcgaacgt gcgggtcgcg gactcgtagt tgaagtcggc cagccgctgc
     4861 cgcatgtcgc cgaacactcc cgcgttgttg atcaccaggt cgatcggcag gtcaccgagc
     4921 gcatcggcga acgtggccac gttcgcgccg tcggcgacgt cgaccgcgtg cagccgcgct
     4981 cccgtcgcgc gcagctcccg cgcgtcgtcg gggcgccggc aggccgcctc gacctggtcg
     5041 ccccgggcga gcagctggcg cacgagctcg agtccgatac cgcggttggc tccagttact
     5101 acgacgttca tggtcgtacg ctacgcgaca gtgtgccgcg gcgcgttctg cgttccttga
     5161 caatcgatat gctgattgct gactcgagct ggctattccg ttcatgaagc gaaacgctgc
     5221 agtggacgag cgccgtgcaa cggactcggg aatctgagtc gcccgcgcgc ccaggcacgg
     5281 ttggcgatgc cgcatccgag ggg
//
LOCUS       DASVOR010002174         5190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_285286,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002174 DASVOR010000000
VERSION     DASVOR010002174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285286"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..956
                     /gene="ftsY"
                     /locus_tag="VF516_40350"
     CDS             <1..956
                     /gene="ftsY"
                     /locus_tag="VF516_40350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEX8114073.1"
                     /translation="PEPAPAVDREAREAYAAGLAKTRGGFVARLGKLFGKKKIDAQTI
                     DELEEVLFTADIGPRAADRIFQAVKSGLSRDELEDPARIWDRIRKTSRDILSVDAPPL
                     DLSGKRPFVLLVLGVNGVGKTTTIGKLAQKWRLDGKKVLLAAGDTYRAAATEQLEVWA
                     ERADVPIVRGKPGGDPSSVIFDAVKKGVDEGMDIVICDTAGRLHSNAGLMDELKKLRR
                     VASKAMPGAPHETWMVLDATTGQNAISQAKTFKADMEISGIVLTKLDGSAKGGVVLGI
                     CDELKVPVRFVGVGEKIGDLRPFDPTAFVDALYADASAAEN"
     gene            1088..2131
                     /locus_tag="VF516_40355"
     CDS             1088..2131
                     /locus_tag="VF516_40355"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HEX8114074.1"
                     /translation="MIAVLAAMAGTAVAQSKGKKPAKPAKAPAKQPAPAQAPAPAATP
                     APDKGSGDNTEIEINPDDTAGAKGSAAGAKGSAAGAGSGSAAVNPDEIDMSEPENKQG
                     DLKSDLAITGNTAPVAATPIKKTPLSWQDIVVVIRKPFLKVRRTELFPFVGTTMNDNM
                     IRHYTAGGELAYYLTDVLAVGVEGQYYAHSFQEPYDLVARQARRLPTLNEYKWSAALD
                     FHYVPIYGKFAILDRQLVTWESFFTAGIGAGESEVIPRDTKFPGFTNLLIMPNVGASM
                     RFFITKSITVNLGIRDYIFYDHFEPRNRSETMFATAADAKAHAEGSLINNLMFQIGVS
                     FWVPTSFEYTTFR"
     gene            2142..3023
                     /locus_tag="VF516_40360"
     CDS             2142..3023
                     /locus_tag="VF516_40360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114075.1"
                     /translation="MKTHSVLAVVAAFAVSGAVSAPVRAERRQNTLAADVAVRHRRLL
                     VKNRFEFTPLFESTINADFRHVLGGGAKLEYHLSDMLSIGVIGVASTSLDTKLVDKIV
                     PTLPTQIQKQMDVAAGKNVRDPSQDEFLDRLNTMPFHGAVYASLTPWYGKLAAFGQAY
                     VAFDFYFQAGVAFAQLKSDCPMTICSDPSPGQSRPDPKNPGDTIAADLNPNNDPPLNS
                     GGRVGLYLGGGIHVFLNDFLALDLTVRDYAFTDNPSGADFNADLFVSTADNRFLHHLF
                     FGAGISVMLPTAVKRTP"
     gene            3142..3525
                     /locus_tag="VF516_40365"
     CDS             3142..3525
                     /locus_tag="VF516_40365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114076.1"
                     /translation="MRSPAAIAGVLVGLLIGGCITPSIPIPPPDPVLMTFVVTGDGAN
                     TSATFSYPADHNYVGSIVFVYNRDRGLGIIEATRPDGSVGPTQPVKAALGDQIVVTFQ
                     RDDLTVSTCVRLRNGTQSAADPCSL"
     gene            complement(3557..4066)
                     /locus_tag="VF516_40370"
     CDS             complement(3557..4066)
                     /locus_tag="VF516_40370"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8114077.1"
                     /translation="MIAPVDNTPGAPVLRREALLPNERSRGLGTVAIVCTMAGVASGL
                     ALATTLMAMQVADSMSSRGCPYREAQIVPQVDLRTDHGFLGVTYYPRDGYADIEQVLP
                     GTPAAAADLHPGDHVLAVNHIALGSPSDLTERIFECHPGDTATLLVERHGERLTVRPT
                     LVAWPTISY"
     gene            4270..>5190
                     /locus_tag="VF516_40375"
     CDS             4270..>5190
                     /locus_tag="VF516_40375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114078.1"
                     /translation="MNTPVPRRRRRWLTAIGLALVVVVTAAYGALRARFSGAELGDNI
                     ASLLNKRMRGRIEVGSVEWSSASLQKVLTGGWVPVTIRDVRVWDDCALSAATTADEAD
                     ELRTGDPNKDCTPDDRPDPDPTSRRKPRKLLLRTDLITAEIDIHAVMFGHHDFVFRNL
                     WVHGGEALLEQTREPYPLHAYARTIVSLVTAFYPRQTPGFRAGITADTPPPIFDLRDI
                     HIAGLNLTLHMSPYTLARPTSVGYGFTARLEGVDVDAGADPANSSYLYMDPTDPLVAK
                     FYVRLAVMARRGTVRILDEGARAAFRLPARG"
BASE COUNT          839 a         1889 c         1690 g          772 t
ORIGIN      
        1 cccccgagcc cgcgcccgcg gtcgaccgcg aggcccgcga ggcctacgcc gccgggctcg
       61 ccaagacccg cggcggcttc gtcgcccggc tcggcaagct gttcggcaag aagaagatcg
      121 acgcgcagac gatcgacgag ctcgaggagg tgctgttcac cgccgacatc ggcccgcgcg
      181 ccgccgaccg gatcttccag gcggtcaagt ccgggctgtc gcgcgacgag ctcgaggacc
      241 ccgcccggat ctgggaccgg atccgcaaga cctcgcgcga catcctgtcg gtcgatgcgc
      301 cgccgctcga tctgtccggc aagcggccgt tcgtgctgct cgtgctcggc gtcaacggcg
      361 tcggcaagac cacgacgatc ggcaagctcg cccagaagtg gcggctcgac ggcaagaagg
      421 tgctgctcgc cgccggcgac acctaccgcg ccgccgcgac cgagcagctc gaggtgtggg
      481 ccgagcgcgc cgacgtgccg atcgtgcgcg gcaagccggg cggcgatccg tcgtcggtga
      541 tcttcgacgc ggtcaagaag ggcgtcgacg agggcatgga catcgtgatc tgtgacacgg
      601 ccggccgcct gcactccaac gcggggctga tggacgagct caagaagctc cgccgggtcg
      661 ccagcaaggc gatgcccggc gcgccccacg agacctggat ggtgctcgac gccacgaccg
      721 gccagaacgc gatctcgcag gccaagacgt tcaaggccga catggagatc tcgggcatcg
      781 tgctcaccaa gctcgacggc tcggccaagg gcggcgtggt gctagggatc tgtgacgagc
      841 tgaaggttcc ggtgcgattc gtcggcgtag gggagaagat cggcgacctg cggccgttcg
      901 atcccacggc atttgtcgac gccctctacg ccgacgccag cgctgcggag aattgacccg
      961 cgcggcagcc gcgctgcaac gcctgcgacg gcgcgctgtc tccattcgtg cgccgtttgc
     1021 ttgagcgcgg aaaaaggcac gtgttatgaa gagcctacga atgcgggcgc ctcgtagatc
     1081 caccttgatg atcgccgtgc tcgctgcgat ggcgggcacg gcggtcgcac aatcgaaagg
     1141 gaagaaaccc gccaagccgg ccaaggcccc cgccaagcag ccggcgccgg ctcaggcgcc
     1201 ggctccggcg gcgacgccgg ccccggacaa gggcagcggc gacaacaccg agatcgagat
     1261 caaccccgac gacaccgccg gcgcgaaggg ctccgccgcc ggcgcgaagg gctccgccgc
     1321 cggcgccggg tcggggtccg cggcggtcaa ccccgacgag atcgacatga gcgagcccga
     1381 gaacaagcag ggcgatctca agtcggatct ggcgatcacc ggcaacacgg cgccggtcgc
     1441 ggccacgccg atcaagaaga cgccgctgtc gtggcaggac atcgtcgtcg tgatccgcaa
     1501 gccgttcctc aaggtccggc gcaccgagct gttcccgttc gtcggcacga cgatgaacga
     1561 caacatgatc cggcactaca ccgccggcgg cgagctcgcc tactacctga ccgacgtgct
     1621 ggcggtcggc gtcgagggcc agtactacgc ccactcgttc caggagccgt acgacctggt
     1681 cgcgcgccag gcgcgccggc tgcccacgct caacgagtac aagtggagcg cggcgctcga
     1741 cttccactac gtgccgatct acggcaagtt cgcgatcctc gatcgccagc tggtgacctg
     1801 ggagtcgttc ttcaccgccg ggatcggggc gggcgagagc gaggtgatcc cgcgcgacac
     1861 caagttcccc gggttcacca acctgctgat catgccgaac gtcggcgcct cgatgcggtt
     1921 cttcatcacc aagtcgatca ccgtcaacct ggggatccgc gactacatct tctacgacca
     1981 cttcgagccc cggaaccgct cggagacgat gttcgcgacc gccgccgacg ccaaggctca
     2041 cgccgagggc tcgctgatca acaacctgat gttccagatc ggcgtgagct tctgggtccc
     2101 gacatcgttc gagtacacca ccttccgctg aagggcatgc catgaagacc cactccgtcc
     2161 tggccgtggt ggcggcgttc gcggtgtccg gcgcggtatc agccccggtc cgcgccgagc
     2221 gccgtcagaa caccttggcc gccgacgtcg cggttcgcca ccgccgcctg ctggtcaaga
     2281 accgcttcga gttcacgccg ctgttcgagt cgacgatcaa cgccgacttc cgccacgtcc
     2341 tcggcggcgg cgccaagctc gagtaccacc tcagcgacat gctctcgatc ggcgtgatcg
     2401 gcgtggcgtc gacctcgctc gacaccaagc tggtcgacaa gatcgtcccg acgctgccga
     2461 cccagatcca gaagcagatg gacgtcgccg ccgggaagaa cgtccgcgac ccgagccagg
     2521 acgagttcct cgaccggctc aacaccatgc cgttccacgg cgcggtctac gccagcctga
     2581 cgccgtggta cggcaagctc gccgcgttcg gccaggccta cgtcgcgttc gacttctact
     2641 tccaggccgg cgtcgcgttc gcacagctca agtcggactg tccgatgacg atctgcagcg
     2701 atccgtcccc cggccagtcg cgccccgatc ccaagaaccc gggcgatacg atcgccgccg
     2761 acctcaaccc caacaacgat ccgccgctca acagcggcgg ccgggtcggc ctctacctcg
     2821 gcggcggcat ccacgtgttc ctcaacgact tcctcgcgct cgacctgacg gtccgcgact
     2881 acgcgttcac cgacaacccg tcgggggccg acttcaacgc cgacctgttc gtgtcgaccg
     2941 ccgacaaccg cttcctccac cacctgttct tcggcgccgg catctcggtg atgctgccga
     3001 ccgcggtcaa gcggacgccg tagggacatc cggccgacct ccgcgttgcc ttctgccaac
     3061 caggttggca cccggagcag ctatccacgc gagaacacgc gactccacgt ggatcgcggc
     3121 ttgcttttct ccccgacggt catgcgttcg cccgccgcca tcgccggtgt cctcgtcggc
     3181 ctcctgatcg ggggctgcat cacgccgtcg atcccgatcc cgccgcccga tccggtgctg
     3241 atgaccttcg tggtcacggg cgacggcgcc aacaccagtg cgacattctc ctacccggcc
     3301 gaccacaact acgtgggctc gatcgtgttc gtctacaacc gggaccgcgg cctcggcatc
     3361 atcgaggcga cccggccgga cggtagcgtc ggcccgaccc agccggtcaa ggccgcgctc
     3421 ggcgaccaga tcgtcgtgac cttccagcgc gacgatctca ccgtgtcgac ctgcgtgcgg
     3481 ctgcgcaacg gcacgcagtc agccgccgac ccctgcagct tgtaggtcgg cgcgccgcgt
     3541 cgcgcggcgt cgagcctcag tacgagatcg tcggccacgc caccagcgtg ggccgcaccg
     3601 tcagccgctc gccgtggcgc tcgaccagca gggtcgccgt gtcgccgggg tggcactcga
     3661 agatccgctc ggtgaggtcg ctgggcgagc cgagcgcgat gtggttgacc gccagcacgt
     3721 ggtcgccggg gtggaggtcc gccgccgccg cgggcgtacc cggcagcacc tgctcgatgt
     3781 ccgcgtagcc gtcgcgcgga tagtaagtaa cccccaggaa tccgtgatcc gtgcggaggt
     3841 cgacctgagg cacgatctgc gcctcgcggt acgggcaccc tcgcgacgac atgctgtcgg
     3901 cgacctgcat cgccatgagc gtggtggcga gcgcgagccc cgaggccacg ccggccatcg
     3961 tgcacacgat ggcgacggtg cccagcccgc ggctgcgctc gttcggcagc aacgcctcgc
     4021 gccggaggac cggggcgccg ggcgtgttgt ccactggggc gatcatctcg ggcaactcgt
     4081 cagacatctc gattccctcg ctttcggtcc agtgttcgac ccgtcgatgc gggtcgcttc
     4141 ggccgtgcct acgtcgagta taggggggtc cccccaacat gaccacgacg aaatgcgccg
     4201 cgatgacgca atcgtgaagc cgcgcacgcc cgagtgcgtc gagtggccat ggtagattcc
     4261 ggagctccgg tgaacacgcc cgtccctcga cgcaggcgcc ggtggctcac cgccatcggc
     4321 ttggcgctgg tggtggtggt gaccgccgcg tacggcgcgc tgcgcgcgag gttctcgggc
     4381 gccgagctcg gcgacaacat cgcgtcgctc ctcaacaagc ggatgcgcgg ccggatcgag
     4441 gtcggatcgg tcgagtggtc gagcgcgtcg ctgcagaagg ttctcaccgg cggctgggtc
     4501 ccggtgacga tccgcgacgt tcgcgtgtgg gacgattgcg cactgagcgc tgcgaccacg
     4561 gcggacgagg ccgacgagct gcgcaccggc gaccccaaca aagactgtac gccggatgac
     4621 cgtccagatc ccgacccgac atcgcggcgc aagccgcgca agctactgct gcgcaccgat
     4681 ctgatcactg ccgagatcga catccacgcg gtgatgttcg gccaccacga cttcgtgttc
     4741 cgcaacctgt gggtccatgg cggcgaggcg ctgctcgagc agacccgcga gccgtacccg
     4801 ctgcacgcct acgcccgcac catcgtcagc ctggtcaccg cgttctaccc ccgccagacg
     4861 ccggggttcc gcgccgggat caccgccgac accccgccgc cgatcttcga cctgcgcgac
     4921 atccacatcg ccgggctcaa cctcacgctg cacatgtcgc cgtacacgct ggcccggccg
     4981 acctcggtcg gctacggctt caccgcgcgg ctcgagggcg tcgatgtcga cgccggcgcg
     5041 gacccggcga acagctcgta cctctacatg gatccgaccg acccgctggt cgccaagttc
     5101 tacgtccggc tcgcggtcat ggcgcggcgc ggcacggtgc ggatcctcga cgagggcgcg
     5161 cgcgccgcgt tccggctgcc ggcgcgcggc
//
LOCUS       DASVOR010002175         1976 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_491121,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002175 DASVOR010000000
VERSION     DASVOR010002175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491121"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /locus_tag="VF516_40380"
     CDS             complement(<1..1059)
                     /locus_tag="VF516_40380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEX8114079.1"
                     /translation="MHTVGLGLGAALVIGGCGVEPGEPQTSEASSELSVNSFVTSSCT
                     TAVVIGLSKQIASEISCMNPTSLTRFAAGSGITITSNAVLPFLAANAKADLEKVGDVH
                     VNSAFRTIAQQYLLLQWFNRGRCGITAAAAVGRSNHESGRAVDLANWSARVSAMASHH
                     WSHDVPGDPVHFDHLSSADIRGKDVLAFQRLWNRNHPNDRIATDGDYGPQTEARLKQS
                     PATGFATGVSCAPKAKVAEIVAADGPDRIAPGQRAHYTLTVSNDTDADWPDTTRVVIA
                     DGTASPLYDATTWASAVEVGPIGAPVPAGTQATVELDVVGPSVATATPIATQLALVDG
                     TTPVGSVDFAVTVTPDGNA"
     gene            complement(1273..>1976)
                     /locus_tag="VF516_40385"
     CDS             complement(1273..>1976)
                     /locus_tag="VF516_40385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114080.1"
                     /translation="PHGERHLQLAALIEAHTGFEFEAAADLLTRTSKVDDIAPDVSVY
                     PAAPDPRTGGRQLEQLAFEIISTQSLGRATAKAVKLTARGVRRVLAIDVERSRVLEWS
                     IDRGTWHELDAAGHIADPALEVPLPIEAMVHAAKADDAVARALVARHNLVIEAVRAED
                     RAAGKAEGKIEGKIEGKIEGKIEALFAILAARDIDPDDAARERILGERDPQRLDRWMA
                     RAALSATLAEVLDSE"
BASE COUNT          267 a          677 c          717 g          315 t
ORIGIN      
        1 cgcgttgccg tcgggggtga cggtgaccgc gaagtcgacg ctgccgaccg gcgtcgtgcc
       61 gtcgaccagc gcgagctggg tcgcgatcgg tgtcgcggtc gcgacgctcg ggccgacgac
      121 gtcgagctcg accgtggcct gcgtcccggc gggcaccggg gcgccgatcg ggccgacctc
      181 gacggcggac gcccaggtcg tggcgtcgta cagcgggctg gcggtgccgt cggcgatcac
      241 cacccgcgtg gtgtccggcc agtcggcgtc ggtgtcgttg ctcacggtca gcgtgtagtg
      301 cgcgcgctgg ccgggggcga tccggtcggg gccgtcggcc gcgacgatct cggcgacctt
      361 cgccttcggc gcgcacgaca cgccggtcgc gaacccggtg gccggcgatt gcttgagccg
      421 ggcctcggtc tgcgggccgt agtcgccgtc ggtcgcgatc ctgtcgttcg ggtggttgcg
      481 gttccacagc cgctggaacg cgagcacgtc cttgccgcgg atgtcggccg agctcaggtg
      541 gtcgaagtgc accggatcgc cgggcacgtc gtgcgaccag tggtggctcg ccatcgcgct
      601 gacccgcgcg ctccagttcg cgaggtcgac cgcgcggccg ctctcgtggt tggagcgacc
      661 gaccgcggcc gcggcggtga tgccgcaccg cccgcggttg aaccactgca gcagcaggta
      721 ctgctgtgcg atggtgcgga acgcgctgtt gacgtgcacg tcgccgacct tctcgaggtc
      781 cgccttggcg ttcgcggcga ggaacggcag caccgcgttg ctcgtgatcg tgatcccgct
      841 gcccgccgcg aaccgggtca ggctcgtcgg gttcatgcag ctgatctcgc tggcgatctg
      901 cttggacaga ccgatcacca ccgcggtcgt gcacgagctg gtgacgaacg agttcaccga
      961 cagctccgag ctggcctcgc tggtctgggg ctcgccgggc tccacgccgc agccgccgat
     1021 cacgagcgcg gccccgagac cgaggccgac cgtgtgcaac ttgcaggttg cggtcggccg
     1081 agctgaaccg gacaaatgac ggaaaccgtg tgcgcttgtc atggtggctg cgagccacag
     1141 caagcggcgc gcccaccgcg acgcggcgac gatcacaccg acggctcgat cgagcagacc
     1201 atcgatacga tcgagagcgc cgcaagggct tcacgaagct cgtgtgcagc gcccgcagcg
     1261 tcacctcacc gatcactcgc tgtcgagtac ctcggcgagg gtcgcagaga gcgccgcgcg
     1321 ggccatccag cgatcgagtc gctgtggatc gcgttcgccg aggatccgct cgcgcgcggc
     1381 gtcgtccgga tcgatgtcgc gggcggcgag gattgcgaat agcgcctcga tcttgccctc
     1441 gatcttgccc tcgatcttgc cctcgatctt gccctccgcc ttgccggcag ctcgatcctc
     1501 cgcccggacc gcctcgatga caaggttgtg cctggccacc agggcgcggg cgaccgcatc
     1561 gtcggccttg gcggcgtgaa ccatggcctc gatgggcagc ggcacctcga gcgcgggatc
     1621 tgcgatatgg cccgcggcgt cgagctcgtg ccaggtgccg cgatcgatcg accactccag
     1681 gacgcgcgat cgctcgacgt cgatcgcgag cacacggcgc accccacgcg cagtgagctt
     1741 gaccgccttg gcggtggcgc ggccgagcga ctgggtgctg atgatctcga acgcaagctg
     1801 ctcgagctgg cgaccgcccg tcctgggatc cggcgcagcg gggtacacgc tgacatcagg
     1861 cgcgatgtca tcgaccttgg aggtccgggt cagcagatcg gccgcggcct cgaactcgaa
     1921 cccggtgtgg gcctcgatca gggcggcgag ctgcaggtgg cgctcgccgt gaggtg
//
LOCUS       DASVOR010002176         2498 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_24662,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002176 DASVOR010000000
VERSION     DASVOR010002176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24662"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..961)
                     /locus_tag="VF516_40390"
     CDS             complement(<1..961)
                     /locus_tag="VF516_40390"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HEX8114081.1"
                     /translation="MATSKNIVDGFPALKKLGFPTGKRAVPFVQQLEWTDCGAASLCM
                     VMAYYGRETKLAEVRDAMGIGRDGVSAKAILDTAERYGLTGRGIKVDTSQIKLVKTGT
                     ILHWEFNHFVVFDRIVPGGIRIVDPATGPRDVPLDQFGKAFTGVAIELIPTDRFTKTK
                     REKGRLTRYVQELLSEKGLASRIIIISLALRLIALALPLITGMIIEQVVPRADYDLLY
                     VILAAVGGMTAFNLIADIVRMHLLLHLRIALDTRMTLGFLDHMVSLPFQFFQRRSTGD
                     LMMRVDSNGIVRETVTGKSMSAVLDGVFVLLYAIVIFYVSPLLG"
     gene            complement(1057..>2498)
                     /locus_tag="VF516_40395"
     CDS             complement(1057..>2498)
                     /locus_tag="VF516_40395"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEX8114082.1"
                     /translation="AAPVALPGGIDALDPMATPTPPELAGSIYAHIRRLALQADLAGA
                     DRVLRDALAELTSSLSCTIVYPGQDGLWTLGADDELPKDTQPLVAAATARRALIASHS
                     AVIPVITTTETVAVIMLTRNARQPAYQPVEQVAMVALAREAAPILHHLAVQHLQRASE
                     IKADKGSLYRGEALEAHRNRGQEGQLVHLTPGWVRRAYPMLVLAILIAVGFAVFVKVP
                     TYSTGTAVVVFEGSTSVTAAIGGTVEKVFVKENEAVKKGDPLVRLNTPDEQAQLTAAD
                     TEWRDQQIQFLFDPTDEQARKAMKSAATQRDQALARVEARTIRATRDGVVTNLHVKEG
                     VAVQPGGYMMQITDENSEIEVLAFLPSKDSPRIREKMGLQLDLDGYRKARVVGTITHV
                     QPDAVSGTEAAKLGGEMLAESIARELQASAQTTSWITARARLPSRMFKAEHNTYHYHH
                     GMHAKVEVKLQSKPFLATLLPGLQKYLPE"
BASE COUNT          352 a          795 c          949 g          402 t
ORIGIN      
        1 cgccgagcag cgggctgacg tagaagatca cgatcgcgta gagcagcacg aacacgccgt
       61 cgagcaccgc cgacatcgac ttgccggtca cggtctcgcg cacgatgccg ttggagtcga
      121 cccgcatcat caggtcgccg gtcgagcggc gctggaagaa ctggaacggc agcgacacca
      181 tgtggtcgag gaagccgagc gtcatccggg tgtcgagcgc gatccgcagg tggagcaaga
      241 ggtgcatgcg gacgatgtcg gcgatcaggt tgaacgcggt catcccgccg accgccgcga
      301 ggatgacgta gagcaggtcg tagtcggcgc gcggcacgac ctgctcgatg atcatgccgg
      361 tgatcagcgg cagcgcgagc gcgatcaggc ggagcgcgag cgagatgatg atgatccggg
      421 acgccaggcc cttctcggac aacagctcct ggacgtagcg ggtcagccgg cccttctcgc
      481 gcttggtctt ggtgaaccgg tcggtcggga tcagctcgat cgcgacgccg gtgaacgcct
      541 tgccgaactg gtcgagcggc acgtcgcgcg ggccggtcgc cggatcgacg atccggatcc
      601 cgcccgggac gatgcggtcg aacaccacga agtggttgaa ctcccagtgc aggatcgtgc
      661 cggtcttgac cagcttgatc tgcgaggtgt cgaccttgat gccgcggccg gtcaggccgt
      721 agcgctcggc ggtgtcgagg atcgccttgg ccgacacccc gtcgcggccg atccccatcg
      781 cgtcgcggac ctcggcgagc ttggtctcgc ggccgtagta ggccatgacc atgcacagcg
      841 aggcggcgcc gcagtcggtc cactcgagct gttgcacgaa cggaacggcg cgcttgccgg
      901 tggggaagcc gagcttcttg agcgcgggaa agccgtcgac gatgttcttg ctggttgcca
      961 tgggtggtga atccctgtgg gcgatggcgg gcgggcgatg ctgggcgggg cgaaccggag
     1021 gcggccagtg ggcgctggcc ggatccggcg tgggcgtcac tcgggcaggt acttctggag
     1081 tccgggcaag agcgtggcga ggaacggctt ggactggagc ttgacctcga ccttggcgtg
     1141 catgccgtgg tggtagtggt aggtgttgtg ctcggccttg aacatccgcg acggcagccg
     1201 ggcgcgcgcg gtgatccagg acgtggtctg cgcgctggcc tggagctcgc gggcgatcga
     1261 ctcggcgagc atctcgccgc cgagcttggc ggcctcggtg ccgctgaccg cgtcgggctg
     1321 gacgtgggtg atcgtgccga ccacccgggc cttgcggtac ccgtcgagat cgagctggag
     1381 ccccatcttc tcgcggatcc gcgggctgtc cttgctcggc aggaacgcca gcacctcgat
     1441 ctcgctgttc tcgtcggtga tctgcatcat gtagccgccg ggctggaccg cgacgccctc
     1501 cttgacgtgg aggttggtga ccacgccgtc gcgcgtcgcg cggatcgtgc gggcctcgac
     1561 ccgggcgagc gcctggtcgc gctgggtcgc cgccgacttc atcgccttgc gggcctgctc
     1621 gtcggtcgga tcgaacagga actggatctg ctggtcgcgc cactcggtgt cggcggcggt
     1681 gagctgggcc tgctcgtccg gggtgttcag ccggaccagc ggatcgccct tcttgaccgc
     1741 ctcgttctcc ttgacgaaca ccttctcgac cgtcccgccg atcgccgcgg tgaccgaggt
     1801 cgagccctcg aacaccacga ccgcggtgcc ggtcgagtag gtcggcacct tgacgaagac
     1861 cgcgaagccg accgcgatca ggatcgcgag cacgagcatc gggtacgcgc ggcggaccca
     1921 gcccggcgtg aggtggacca gctggccctc ctggccgcgg ttgcggtggg cctcgagcgc
     1981 ctcgccgcgg tacagcgagc ccttgtcggc cttgatctcg ctggcgcgct gcaggtgctg
     2041 gacggcgagg tggtggagga tcggcgcggc ctcgcgggcg agcgcgacca tcgcgacctg
     2101 ctcgaccggc tggtacgccg gctggcgcgc gttgcgggtc agcatgatga ccgcgacggt
     2161 ctcggtggtg gtgatcaccg ggatcaccgc gctgtggctg gcgatcagcg cgcggcgcgc
     2221 ggtcgcggcc gcgaccagcg gctgggtgtc cttggggagc tcgtcgtcgg cgccgagcgt
     2281 ccacaggccg tcctggccgg gatagacgat cgtgcaggac agcgacgagg tgagctcggc
     2341 gagcgcgtcg cgcagcacac ggtcggcgcc ggcgaggtcg gcctgcagcg cgagccggcg
     2401 gatgtgggcg tagatcgatc cggcgagctc gggcggcgtc ggggtcgcca tcggatcgag
     2461 cgcgtcgatc ccgccgggga gcgcgaccgg cgcggcag
//
LOCUS       DASVOR010002177         3793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_285344,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002177 DASVOR010000000
VERSION     DASVOR010002177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285344"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1093)
                     /locus_tag="VF516_40400"
     CDS             complement(35..1093)
                     /locus_tag="VF516_40400"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8114083.1"
                     /translation="MGKPRVAMPGETAQRRSSDGVTTAPVMDGRLRMIGNGKPLETEI
                     RDRVEKFFGADFSGVRVHVTPAVPAMGALALTLGETLYFAPGLYDPTTREGVELLGHE
                     LTHVVQQRSGRVVNPYGRGVAIVQDPELEAEADSMGRQMAEHIWPQRGIAQAAMKGRA
                     GRGTLQASASNQAPALEPPLAAAQFVGKNALIQASYKNFIPHVGKFLDCGMKIRGLLE
                     QLRKDLGTNAVDQLGLAIRIVRNKDRNKTIKLEGGWEGEYHVYMRSEDVSGRDLLYDP
                     YINASNCRGNTSEDTALPVLWETDMGELERITVAGVGTDIKVTEYMECYQGNWGSLVK
                     HAKEVQTDPSVRGEYIDW"
     gene            1849..3033
                     /locus_tag="VF516_40405"
     CDS             1849..3033
                     /locus_tag="VF516_40405"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8114084.1"
                     /translation="MGAGFDRKRGDGTLAPNHHGSASGTAPGKRTLTEELPPVQRSAS
                     STSAQSTDGQATQQAAAQGVAGGGGPLPHGETIQRLFGRHDVSGIEAHVGGPAAAASR
                     AIGAEAYATGHHVAFASTPGLHTAAHEAAHVVQQRGGVQLQGGVGAAGDAYEQHADEV
                     ADRVVRGESAETLLDAAPGGAAVADHAVQRKTTVTDDPADATNKTAPGTGAGLLKNTA
                     GTTLPKSDVGFGPLVNGCGSSVTAWRYPGDDVAGSTPSVRPSWWAAMMADPNTDSAWV
                     SNNVVQGHLLNEHLGGPGSDMRNLTPFAKSTNSQHHAYVEKKAKAIFGRGNIVRYKVT
                     VNYGSPPPAAWFGNKIPATYLAHFAQSIVCILDEHDKSTGQSITGKEDVTNITNAMTG
                     QG"
     gene            3051..3560
                     /locus_tag="VF516_40410"
     CDS             3051..3560
                     /locus_tag="VF516_40410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114085.1"
                     /translation="MSALWSARVLAKDNRRLVVELRAIHPDSGEFLTSKRFALRLLYD
                     EAYGYGPGSVRESRGPLGDAIALEQTFDDTFMDASVDRFVERVGVDVRTPRLDVEALR
                     DRIDRELASRGTHRDDRAAWRTAWDELWDAFWADPENLPVATYTVDVTDPRWIAHLTI
                     GSTWESATY"
BASE COUNT          633 a         1344 c         1216 g          600 t
ORIGIN      
        1 ccgccggatc ttcgcggcgt ggtgacggta gggactacca atctatatat tcccccctga
       61 cgctgggatc cgtctgtact tccttggcgt gtttcaccaa cgaaccccag ttcccctggt
      121 agcactccat gtactcggtc accttgatgt ccgtcccgac gccggcgacg gtgatgcgct
      181 ccagctcccc catgtcagtt tcccacaaaa ccggcaaggc cgtatcctcg gacgtgtttc
      241 cgcgacagtt cgatgcgttg atataaggat cgtacagcaa gtccctgccg ctgacatctt
      301 cactccgcat atagacgtgg tattcgccct cccatcctcc ttcgagtttg atggtcttgt
      361 ttctatcctt gtttcgcacg atgcgtatgg ccaaccccag ttgatccacc gcattcgttc
      421 ccaggtcttt tctcaattgc tcgagcagtc cccgtatctt catgccacaa tccagaaact
      481 tgccgacgtg tggaatgaaa ttcttgtagc tggcttgaat caatgcattc ttgccgacga
      541 actgggcagc ggccagtggc ggctcgagcg ccggggcctg attgctggcc gacgcctgta
      601 gcgtgcctcg tcccgctcgc cccttcatcg ccgcctgggc aatccccctc tgcggccaga
      661 tgtgttctgc catctgccgc cccatcgagt ccgcctccgc ctccagctcc ggatcctgca
      721 cgatcgccac cccgcggcca tacgggttca cgacccgtcc actccgctgc tgcaccacat
      781 gcgtcagctc gtgtccgagc aactctaccc cctcgcgcgt ggtcggatcg tacagccccg
      841 gcgcgaaata cagggtctcc cccagcgtga gcgccagcgc tcccatcgcc ggcaccgccg
      901 gcgtgacatg cacccgcaca ccggagaagt cggccccgaa aaacttctcc acccggtctc
      961 ggatctcggt ttccagtggc tttccattgc cgatcatccg cagccgtccg tccatcaccg
     1021 gcgccgtcgt caccccatca ctcgaccgtc tctgtgccgt ctcgcccggc attgccaccc
     1081 gcggcttgcc catcccgggg agccgttcgg gccgcggagc caaggtccac cggggtgcgc
     1141 cccgggcctg cgcgatcggt gcccctgtcc gcgctaggat cggatccgga cgcggccccc
     1201 tgcccggctc ggccacgctc ctggcccatg cggccggttc tcgcggccgc gcgggcatca
     1261 gctccggccg cggcgcgttc gcgagccagt cgttccgagg tcggggctgg accaccgcgc
     1321 tcctgcaccc ggccccgcga aaccgctcga tgcagaagta gccctgtaac accaggcctc
     1381 gtctcgatct atacatcggc gtcaatccct cccgagggcg gcggggggcg tgggcggcgc
     1441 gagcagatcg atcacaggcc tggttcccgt acggccgaca gggtcttgca gaacaggtcc
     1501 atgggcacgc tcgtcacctc gacgcctcga cactgggcat gatgatcacc gcaggtccgg
     1561 gcgggcaagt tcccgaccga gagtgcgggg gagcgcctgg gacaacaagg gaaggcctcg
     1621 ccgtgcgccg gcgcacaccg gccggtatgc agggactgcg cggtggcctt cgtcgtgatc
     1681 aggtgcgacg gaaatttccg ggatcccgtg caccctcgga gaacgctccc gcccgcgggc
     1741 ctctcgcacg tgcggcgtct tgcaccacac gcgcggctca cgggaatgcc gtcgacgttc
     1801 accgcagggc cgtgatatca ggcaatcacc gcagccagtg gagtgaagat gggcgcagga
     1861 ttcgatcgaa aacggggcga tggcacgctc gccccgaacc accatggaag cgccagcggc
     1921 acggcacccg gaaagcggac gctcaccgag gagctgcctc cggtacagcg atccgcgagc
     1981 tcgacgtccg cacagagcac cgacggtcaa gcaacgcagc aagcggccgc gcagggtgtc
     2041 gccggtggcg gaggcccact gccccatggc gagacgatcc agcgcctgtt cggccggcac
     2101 gacgtgagcg gcatcgaggc gcacgtcggt ggacccgccg cggccgcgag ccgcgcgatc
     2161 ggggccgagg cctacgcgac cgggcaccac gtcgcgttcg cttccacccc aggtctccac
     2221 accgccgcgc acgaggccgc gcatgtcgtg cagcagcgcg gcggcgtgca gctccagggt
     2281 ggcgtcggcg cagcaggcga tgcgtacgaa cagcacgcgg acgaggtggc ggatcgggtc
     2341 gtgcggggcg agagcgccga gaccttgctc gacgcggccc ctggtggcgc ggcggtggcc
     2401 gatcacgccg tccagcgcaa gaccacggtc accgacgatc ccgcggatgc gacgaacaag
     2461 accgcaccgg gcaccggagc gggcctgctc aagaacaccg caggcacgac gctccccaag
     2521 agcgatgttg gtttcggccc gctcgtcaac ggctgcgggt cctcggtgac ggcgtggcgg
     2581 tacccgggcg acgacgtcgc cggcagcaca ccctccgtgc gcccgagctg gtgggccgcg
     2641 atgatggccg atccgaacac cgacagcgcc tgggtgtcga acaacgtcgt gcagggccac
     2701 ctgctcaacg agcacctggg tggccccgga agcgacatgc gcaacctgac gccgttcgcg
     2761 aagtccacca actcccagca ccacgcgtac gtcgagaaga aagccaaggc gatcttcggg
     2821 cgcgggaata tcgttcgcta caaggtcacg gtgaactacg ggtcgccgcc gccggctgcg
     2881 tggttcggga acaagatccc cgccacgtac ctggcacact tcgcacagtc catcgtatgc
     2941 atcctggacg agcacgacaa gtcgaccggc caatcgatca ccggcaagga agacgtcacc
     3001 aacatcacca acgccatgac gggccagggc tagccggcga ggaggcctcc atgtcagcac
     3061 tgtggtctgc acgcgtgctc gccaaggaca accgccgtct cgtggtcgag ctacgcgcga
     3121 tccatcccga tagcggggag ttcctgacct cgaagaggtt cgcgctccgg ctgctctatg
     3181 acgaggcgta cggctacgga ccgggctcgg tgcgcgagtc ccgaggcccg ctgggcgatg
     3241 cgatcgcgct cgagcagacg ttcgacgaca ccttcatgga cgccagcgtc gatcggttcg
     3301 tcgagcgcgt cggggtcgac gtgcgcacac cgcggctcga cgtcgaggcg ctgcgcgacc
     3361 ggatcgatcg ggagctcgcc agccgcggaa cccaccgcga cgatcgcgcg gcgtggcgca
     3421 cggcctggga cgagctctgg gacgcgttct gggccgatcc ggagaacctg ccggtggcga
     3481 cctacacggt ggacgtcacc gatccccggt ggatcgcaca cctgacgatc ggaagtacct
     3541 gggagtccgc gacgtactga gcgcgatcgg gtccgccatg gcgcgcgggg tgctcgcgcc
     3601 gctcgggggc ccgtgcacct acttggtcgc cgccgccggc agcaggccct ccgccttcat
     3661 cgcggcctgc accgccgggc gcgccgcgac gcgggcctgg aacgcgagca ggttggggaa
     3721 ctcggacagg tcgagcttga ggaagttgga ccagttggtg accacgaaca ggtaggcgtc
     3781 ggcgacggtg aac
//
LOCUS       DASVOR010002178         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_549929,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002178 DASVOR010000000
VERSION     DASVOR010002178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_549929"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..791)
                     /locus_tag="VF516_40415"
     CDS             complement(123..791)
                     /locus_tag="VF516_40415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114086.1"
                     /translation="MLDHKPGKGTHAHGHAAGGPEPHAGKQSRVEQELGGQGHGGPVA
                     TAADTTVSSTFSTDYASFHQGNVFVSESVSATLSHGKGPVKIDLNKGGMSVGNEHMTL
                     NLKSADAATGKHLHVSGASAEIIKNHSSHTGAKIEDGYVGVDYTTSWTVKGTGHHPWS
                     VTLSFSTFIGAKPPHHHHKHWWQHIPVVSTVVAAAAAAIAAAAAAIIASSPEWGPVVV
                     VALA"
     gene            1056..1427
                     /locus_tag="VF516_40420"
     CDS             1056..1427
                     /locus_tag="VF516_40420"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8114087.1"
                     /translation="MKAIEARIEARIEARTVVITGASSGIGRASAVRLARAGFPVYAG
                     LRAEADAEALRAEHPAIEPIAIDVTDATSIAAARHRLERELHGLGGLVNNAGVGLVAP
                     MEHVPLDEIRLRLFGLPRASG"
     gene            1516..>1968
                     /locus_tag="VF516_40425"
     CDS             1516..>1968
                     /locus_tag="VF516_40425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEX8114088.1"
                     /translation="MSSIPRDPALDSTLSVMREGYEFIWNRCRRFHSDLFTARVMGRP
                     AVCIHGREAAELFYDPRKLERRGALPRRVVTSLFGTRAVQTLDDDAHRRRKAAFLSLM
                     GPSRLDELVRETARQWQSAIRSWPGRDRVVVFDEAERVLAAAACSWAGV"
BASE COUNT          255 a          637 c          755 g          321 t
ORIGIN      
        1 gtgcatcgac gccctgcgca ccgcgacgaa ccaggcatgg gcggagcccg gctccatgcg
       61 attccgcgga atcgcttccg acgcatgggg tgcacccgcg ctgctttggt ggcagcgctg
      121 ggctacgcga gcgcgacgac caccacgggg ccccactcgg gggagctggc gatgatcgcg
      181 gcggccgcgg cggcgatcgc ggcggccgcg gcggcgacca ccgtgctgac gaccgggatg
      241 tgttgccacc agtgcttgtg gtgatggtgg ggcggcttgg cgccgatgaa ggtggagaac
      301 gatagcgtca cgctccacgg gtggtggccg gtgcccttga ccgtccacga cgtggtgtag
      361 tcgacgccga cgtagccgtc ctcgatcttg gcgccggtgt gcgacgagtg gttcttgatg
      421 atctcggcgc tcgcgccgct gacgtggaga tgcttgccgg tggcggcgtc ggcggacttg
      481 aggttgaggg tcatgtgctc gttgccgacc gacatcccgc ccttgttgag gtcgatcttg
      541 accgggccct tgccgtggga cagcgtggcg ctgaccgatt cgctgacgaa tacgttgccc
      601 tggtggaacg aggcgtagtc ggtcgagaac gtgctcgata ccgtcgtgtc ggccgccgtc
      661 gccaccggcc cgccgtgtcc ctggccgccg agctcctgct cgacgcggct ctgcttgccc
      721 gcgtgcggct cggggccgcc ggctgcgtgg ccgtgcgcgt gcgtgccctt gcctggcttg
      781 tggtccagca tcgttgtcct cctgggcgcc tttacgcggg caacggttgc ctccttccct
      841 gacggatgcg gagaattccg tcggagaccg ttcacatcgc gatacctgcg gcgctgcggt
      901 ccatcgacgg gcgggcgatc tgtcatgcgg actacatgac ctgacgagct acagggtcga
      961 tctcgtgtcg cggcggtgca tggcgcgctg cggcgccgcg aagctgcggt gccggcaggg
     1021 tttgccggtc atcgagtgcg cggtacgagg gcgagatgaa ggcgatcgag gccaggatcg
     1081 aggccaggat cgaggccagg accgtggtca tcaccggcgc gtcgagcggt atcgggcggg
     1141 cgtcggccgt gcggctcgcc cgggcggggt tcccggtgta cgccggcctc cgcgccgaag
     1201 cggacgcgga ggcgctgcgc gcagagcacc ccgcgatcga gccgatcgcg atcgacgtca
     1261 ccgacgcgac ctcgatcgca gcggcgaggc atcggctgga gcgtgagctc cacgggctcg
     1321 gcggcctggt caacaacgcc ggggtcggcc tggtcgcgcc gatggagcac gtgccgctcg
     1381 acgagatccg gctccggctg ttcggcttgc cccgggcaag cggctagatg acctgagagg
     1441 aatcgcgcgt ggcgccggag cgccggcgtg caccgaccgg gaccgggtat gccgcgtgca
     1501 gctcgttgac gaaccatgtc atcgattcca cgcgatcccg cgctcgacag cacgctgtcg
     1561 gtgatgcgcg agggctacga gttcatctgg aaccgctgcc ggcggtttca cagcgacctg
     1621 ttcaccgcac gggtgatggg ccggccggcg gtgtgcatcc acggccgcga ggccgccgag
     1681 ctgttctacg acccgcgcaa gctcgagcgg cgcggcgcgc tgcctcgccg cgtggtgacg
     1741 tcgctgttcg gcacgcgcgc ggtgcagacg ctcgacgacg atgcgcaccg ccggcgcaag
     1801 gcggcgttcc tgtcgctgat ggggccgagc cggctcgacg agctggtccg cgagaccgcg
     1861 cgacagtggc agagcgcgat ccgcagctgg cccgggcgcg atcgggtggt ggtgttcgac
     1921 gaggccgagc gagtgctggc ggccgcggcg tgcagctggg cgggggtg
//
LOCUS       DASVOR010002179         2238 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_491187,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002179 DASVOR010000000
VERSION     DASVOR010002179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2238
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491187"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(352..900)
                     /locus_tag="VF516_40430"
                     /pseudo
     CDS             complement(352..900)
                     /locus_tag="VF516_40430"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(913..1320)
                     /locus_tag="VF516_40435"
     CDS             complement(913..1320)
                     /locus_tag="VF516_40435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114089.1"
                     /translation="MKILSRAALLAALALAACQQAPSRLDNVTSKAPVPKATASGPTG
                     GDPPAGLKIDRTGSVEQQLARLQDAYDRNAEAMDFLNKVFAQQKAQQQAQEREEPAED
                     ATFAVDIAPDVKLGQVEGPANALVTIVEAWDFA"
     gene            complement(1442..1981)
                     /locus_tag="VF516_40440"
                     /pseudo
     CDS             complement(1442..1981)
                     /locus_tag="VF516_40440"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
     gene            complement(1994..>2238)
                     /locus_tag="VF516_40445"
     CDS             complement(1994..>2238)
                     /locus_tag="VF516_40445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114090.1"
                     /translation="EDRVARLEANYDRYAEALDFLGKVYEQQKAQQKAQAERAQREDP
                     DPDAVFAIDVAPDVKLGEVEGPSTALVTIVEAWDFA"
BASE COUNT          299 a          759 c          735 g          445 t
ORIGIN      
        1 ggcaagcgat gtttcaccag ctcgccggct cggagcaagc agggcatcgc gccggagacc
       61 acgtgcacgc tcggctcgcc gtgggctgct acgcccatcc ggatcgaccg tcgaggttcg
      121 cctcgtggcg cgatcgccag acgtcaacgc gagacttgcg ggggcgaccg cgagcaggat
      181 gggggtcgga cgccgcgacc gtgatctgga ttggccgttc gttgcccccc gcacatacct
      241 gcgccgttcg cgcatgggtc ggtggggatt tggcgggcgc ggctccgaca cccatccgcg
      301 cagcagcccc cggcgagtct cgcgtgtgag cgttctcccg agcgctatgc gctagtgcgg
      361 cttcggatcg accttcgagc ggaactgctt ctcgcccttg ccgatcactt ccttctcgta
      421 gtagccggcg gccggcactc ccgaggcctc agcgatcttc agcttctcgt cgatcacctg
      481 cttgaacgcg tccttgggca gcgcgccgtt gatcggcttg ccgttgatga agaacgacgg
      541 cgtcgagttg acgtgccact tcgcgagctc ggtcatgtcg gtctcgacct gggccttgca
      601 ctcggggctg tccatgtcgg ccttgagctt ggcggcgtcg agcccgatgc cctgggcgat
      661 cttcacgatg ttgtcggcgc cgagcgccgc ctggtccttg ccgcccgacg cggcgtacgg
      721 accgaacgcc ttgtcccaga acgcgttctt gaactcgacg aacttgccct gcttggccgc
      781 ggcgcacccg gcgtggtgcg cgtcggtcac gacctgcggg tgcaccacca tgttcttgaa
      841 taccacccgg accttcccgt tgtactcctt gaccagatcc tcgagggtcg aactgaccct
      901 ctggcagtag ggtcaagcaa agtcccaggc ctcgacgatc gtcaccagcg cgttggccgg
      961 gccctcgacc tggccgagct tgacgtcggg ggcgatgtcg accgcgaacg tcgcgtcctc
     1021 ggccggctcc tcgcgctcct gggcctgctg ctgcgccttc tgctgggcga agaccttgtt
     1081 caggaaatcc atcgcctcgg cgttgcgatc gtaggcgtcc tgcagccgag cgagctgctg
     1141 ctcgacgctg ccggtgcggt cgatcttcag accggccgga ggatcgccgc ccgtcgggcc
     1201 ggacgccgtg gctttcggca ccggggcctt gctcgtgacg ttgtcgaggc ggctcggggc
     1261 ttgctgacac gccgcgagcg ccagcgctgc gaggagcgcc gccctggaga ggatcttcat
     1321 ggcgggggag cgtgccaacc tcgccgccga tcggcaagtc gctggcgagc cgaaccacac
     1381 tccgcctcgc gggttttgtc gtccgggggt ccgggggccc tggagtccag agccgtccgt
     1441 tctacttgcc tgcgtccatc ttcgagcgga actgcttcac gcccttcccc atgatctcct
     1501 tgtcgtaata gctcgctccc gacactccgg aggcctcggc gaccttcagc tgctggtcga
     1561 tgacctgctt gaagccttcc ttgggcatcg cgccgttgag gtgcttgccg ttgatgaaga
     1621 acgtcggggt cgagttgacg tggaacttct ggagctcggc catgtcggcc tggacgtggg
     1681 ccttgcagtc cgggccatcc atgtcggcct tgagcttggc ggtgtcgagc ccgatgtcct
     1741 tggcgatcgc catgatgttg tcgacggcga gcttggacgg gtcgtgctgc gcggcgtacg
     1801 gggcgaacgc tttgtcccag aacgccttct tgaactcgac gaacttgccc tgcttggcgg
     1861 cggcgcaccc cgcgttgtgt gcgtcggtca cgtactgcgg gtggaccacc atgttcttga
     1921 acaccacccg gaccttcccg ttgtactcct tgaccagatc ctcgagggtc gaactgaccc
     1981 tctggcagaa gggtcaagca aaatcccagg cctcgacgat cgtcaccagc gcggtgctcg
     2041 ggccctcgac ctcgccgagc ttgacgtcgg gcgcgacgtc gatcgcgaac accgcatccg
     2101 gatccgggtc ctcgcgctgc gcgcgctccg cctgcgcctt ctgctgggcc ttctgctgct
     2161 cgtagacctt gccgaggaag tcgagcgcct cggcgtagcg gtcgtagttg gcctcgagcc
     2221 gggcgacccg gtcctcga
//
LOCUS       DASVOR010002180         1609 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_228058,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002180 DASVOR010000000
VERSION     DASVOR010002180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228058"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1503)
                     /locus_tag="VF516_40450"
     CDS             complement(<1..1503)
                     /locus_tag="VF516_40450"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8114091.1"
                     /translation="MRRFSERTRIARILAPSVVRSVVHRVGAVLVSSGLLASAAGVAG
                     LTGCEQLDGRNRNKQGLRLFRETQFVEAAAQFQRALGEVDDSPIIHYNLGLAYSKVTK
                     TGYDGPVLLGQQGDFVCQTLPGVKIVQAGACVKEGDRHYAECGSAKTGPLEKDLADLK
                     GQLAAVADDAKKKELKVQIDDKQSDLMRYACGSSFHCVEGPFCSLTSPELADMAAQHF
                     QIWIKAQPSDDEIKKQLAQASEELDEAKKSGNASAIGRAQTKVDDLSTKDQTRKLMTL
                     LWTDSDQYQKAIDYWEGLLKEKPNDAEIMGNLAGIQLKAGNWRKSIEWYTKVAEVTND
                     ASSKVVTYQYIGNVAWQKLNSRLLVGADAIELADRGIGALQRGAEVQPKNARLVGLQA
                     SIYNFRSTSHGASWAAAIDRASAQELLKLSRVLADEAKKAQGQPTGAPETPATPAGTG
                     TPPAGAGSGAPPATPSGGSAAPPAGSAAPPAAPGPSGTSAPAAAPASTAAP"
BASE COUNT          219 a          540 c          548 g          302 t
ORIGIN      
        1 cggggctgcg gtcgatgccg gcgcggccgc cggagccgag gtcccggaag gacccggtgc
       61 cgccggcggg gctgcagagc ctgccggagg cgcggcagag ccgcccgatg gcgttgccgg
      121 cggtgctcca gagcccgcgc ccgccggagg tgtgcctgtg cctgctgggg ttgctggggt
      181 ctctggtgct cctgtcggtt gtccctgggc cttcttcgcc tcatcggcca gtacgcgcga
      241 cagcttcaaa agttcttgcg cgctcgcacg gtcgatcgcg gccgcccagg acgccccgtg
      301 agacgtcgac cggaagttgt agatcgatgc ctgcaggccg accagccgcg cgttcttggg
      361 ctggacctcg gcgccgcgct gcagcgcgcc gatgccgcgg tcggcgagct cgatcgcatc
      421 ggcgccaacc agcagccgcg agttcagctt ctgccaggcg acgttgccga tgtactggta
      481 ggtgaccacc ttgctgctcg cgtcgttggt gacctcggcc accttggtgt accactcgat
      541 cgacttgcgc cagttgccgg ccttgagctg gatgccggcg aggttgccca tgatctcggc
      601 gtcgttgggc ttctccttga gcaggccctc ccagtagtcg atcgccttct ggtactggtc
      661 cgagtcggtc cacagcagcg tcatcagctt gcgggtctgg tccttggtgg acaggtcgtc
      721 gaccttggtc tgcgctctgc cgatcgcgga cgcgttgccc gacttcttgg cctcgtcgag
      781 ctcctccgac gcctgggcga gctgcttctt gatctcgtcg tcgctcggct gcgccttgat
      841 ccagatctgg aagtgctggg cggccatgtc ggcgagctcg ggcgaggtca gcgagcagaa
      901 cggaccctcg acgcagtgga acgacgagcc gcaggcgtag cgcatcaggt ccgactgctt
      961 gtcgtcgatc tggaccttga gctccttctt cttggcgtcg tcggcgaccg ccgcgagttg
     1021 acccttgaga tcggcgaggt ccttctcgag cggcccggtc ttggccgagc cacattcggc
     1081 gtagtggcgg tcgccttcct tcacgcacgc gccggcctgc acgatcttga ccccggggag
     1141 cgtctggcac acgaagtcgc cctgctggcc gagcagcacg ggaccgtcgt acccggtctt
     1201 ggtgaccttc gagtacgcca gcccgaggtt gtagtggatg atcggcgagt catcgacctc
     1261 gccgagcgcc cgctggaact gcgccgcggc ctcgacgaac tgggtctcgc ggaacaaccg
     1321 gaggccctgc ttgttccggt ttctgccatc gagctgctcg cagcccgtca ggccggcgac
     1381 gccggcagca ctggcaagca agccggacga cacgagcaca gcaccgacgc ggtggacgac
     1441 gctgcggacg acgctcgggg cgaggatccg ggcgatgcgt gtgcgctcgc tgaaacgtct
     1501 catggagcgc gcgactatac atagcgaggt gggggtcatc aacggccttc ggctctccat
     1561 ctacctactc tcccatctca taccgcgaca gcgcggcgag gtgacaccc
//
LOCUS       DASVOR010002181         6384 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_374162,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002181 DASVOR010000000
VERSION     DASVOR010002181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6384
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374162"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VF516_40455"
     CDS             <1..1063
                     /locus_tag="VF516_40455"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8114092.1"
                     /translation="WTTDPFKFIEKDGYFYGRGTSDDKYMAVAWVVNMIRWKKEGYKP
                     DRDLVLVLETDEERGDRNSVGMQWLIKNHRDLLDAELALNEGGGVGVKEGKPFVNSIQ
                     TAEKLVQSFWVEARNPGGHSSQPRKDNAIYELADALGRLENHHFPVHLNDTTRAFFEK
                     MAALEHGPLAQDMKSLVSPRPDPAAIDRLTAQPPYNAQIRTTCVATRLEGGHADNALP
                     QLARAMVNCRIVPGETPDQVQKELEATFADPKLTITTVPRDTGSDPSAMNKDLLAAIE
                     KVSPKFWPGVPAVPTMTPGATDGRFLRNVGIPTYGHSGMASDIFDVRAHGKDERVSIQ
                     ATFEGEQYLYELVKALSGG"
     gene            complement(1095..1892)
                     /locus_tag="VF516_40460"
     CDS             complement(1095..1892)
                     /locus_tag="VF516_40460"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8114093.1"
                     /translation="MASPNRGQWTDNSGGDQNQAPQGYSPQGSYSSQGGYSPQGGYQQ
                     PSGYSAQGSSAQGSSTQGYPAPSAPPPGGFQDQGSPQPPDQDQEEDFAETEPVESQPR
                     LGVMVISLTPELRQYFRVAHDRGVLIARVEPNSPAARAGIQVGDILVRVGPFVVHAGD
                     DVIQALAARQGGRLPITVVRQARPVHLVAFISTGTSNQPPMNNPNFQGPMNNQAPPMN
                     NQAPMNNQAPQGPMSNGPINQAPPPNQSNQPITQSNLPNSSSQQNQL"
     gene            complement(2154..3674)
                     /locus_tag="VF516_40465"
     CDS             complement(2154..3674)
                     /locus_tag="VF516_40465"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEX8114094.1"
                     /translation="MNRAVSPRAESPMACRVGLLLAVAVAPACGDNAIYPESVYAERC
                     AQPRAGIDPGTGQPYTDVAGSVMEEKLWVHSWINDLYLWYREVPSVDITKYATAVDYF
                     DQEKTPATTTSGKPKDQFHFIYKTTDWEALSQSGTEASYGIQWALLAPSPPRSLLIAY
                     TEPGSPAAAVQPPLGRGARVLSIDGVDVERGSDVDTLNAGLFPSNLHESHQFVVQDLG
                     ATTTRTVTLTSTSVEIMPVLNVRTLPAPNDKIGYFLFTDHIATAEKALFDAFTQLRAA
                     GVTELILDMRYNGGGYLDIASELAYMIAGPTATAGKTFERLEFNDRYPTTDPFSGLPL
                     QPEGFVNQSLGFVPSRLGAGQPLPSLGLSRVFVLTSSSTCSASESVMNSLAGVNVQVI
                     QIGETTCGKPYGFVPQDNCGTTFFAIQFQGVNNKGFGDYADGFTPGGVFKGCTVADDF
                     THLLGDSAEARIAAALSYRASSTCSQVAPRTADPRAAVTGAVIPKPAWRQNRIVRR"
     gene            4258..4803
                     /locus_tag="VF516_40470"
     CDS             4258..4803
                     /locus_tag="VF516_40470"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX8114095.1"
                     /translation="MGQAIVALLKTGRSAPELANTEIKLFHIRDFQKGLEEGVSVYLY
                     RVTVSGARRSLPPTYSPSGRPYRQPLPVDLYYLVTPWAKTAEMQHLILAWAMRTIEDS
                     PTFPASLLNSFVPDSFRPEESIDLIHDTVSVQDMSYIWEIGKANMQTSATFVARVVAI
                     DSRVEQPDPGPPVQTRVFESR"
     gene            4838..5764
                     /locus_tag="VF516_40475"
     CDS             4838..5764
                     /locus_tag="VF516_40475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114096.1"
                     /translation="MIVRRTWSRVAPLGVRFVDDRSGDLVVDNLVVTAWPAREPSRRS
                     PAIVNRSGVFSLHDLPGLGGLEHGTGDDAYWRAAPRRPFVVDVEDLALRFLAFRFTVE
                     LPLRGLLELRCGSPPAAVGPAPDRGVPLFTAPTRPTLPGMAVLRADLWDAAANAPAAW
                     AVVQASIPSAAAHGVPPIRAIADDRGQIALHMPCPDAEDLDGGSFDSPSGPAPVPLTA
                     RTWSVDLAASYGHLTPGAPIVGLLPSRGGAPIPDLCAAMTQPPATLWSDRARTTPLRR
                     ASLGFGSETVLRSTGSGAGPQSVLFLTPGSPP"
     gene            5808..>6384
                     /locus_tag="VF516_40480"
     CDS             5808..>6384
                     /locus_tag="VF516_40480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114097.1"
                     /translation="MYIEEVSFRSKSIEGVSTTTTGFVGPTRDGPVDLDNELITSLGD
                     FKRVYGDGSQLQFGNDVLHNYMWHAARAFFAEGGKQLYVSRVFRRLDADSASAPPAGT
                     VDRSPSRSGGASGPYFDGYARAGTAGRLSDATAGTSLVVRARWPGAAGNVRVRFGLRV
                     SKSVLGFDQRRDPVSGAATSVAVVSSLADRDV"
BASE COUNT          952 a         2129 c         2298 g         1005 t
ORIGIN      
        1 ctggaccacc gacccgttca agttcatcga gaaggacggc tacttctacg gccgcggcac
       61 cagcgacgac aagtacatgg ccgtcgcgtg ggtcgtgaac atgatccggt ggaagaagga
      121 gggctacaag cccgaccgcg acctcgtgct cgtgctggag accgacgagg agcgcgggga
      181 tcgcaacagc gtgggcatgc agtggctgat caagaaccac cgcgatctgc tcgacgccga
      241 gctcgcgctc aacgagggcg gcggggtcgg cgtcaaggag ggcaagccgt tcgtcaacag
      301 catccagacc gccgagaagc tggtccagag cttctgggtc gaggcccgca acccgggcgg
      361 ccacagctcg cagccgcgca aggacaacgc gatctacgag ctcgccgacg cgctcggccg
      421 gctcgagaac caccacttcc cggtccatct caacgacacc acgcgggcgt tcttcgagaa
      481 gatggccgcg ctcgagcacg gcccgctcgc gcaggacatg aagtcgctgg tcagccccag
      541 gccggatccg gccgcgatcg accggctgac cgcgcagccg ccgtacaacg cccagatccg
      601 caccacctgc gtcgcgaccc ggctggaggg cggccacgcc gacaacgcgc tgccccagct
      661 cgcgcgcgcg atggtcaact gccggatcgt gccgggcgag acccctgacc aggtccagaa
      721 ggagctcgag gccacgttcg ccgatcccaa gctcaccatc accaccgtgc cgcgcgacac
      781 cggcagcgat ccgtcggcga tgaacaagga cctgctcgcg gcgatcgaga aggtcagccc
      841 gaagttctgg cccggcgtcc cggcggtgcc gacgatgacc cccggcgcga ccgacggccg
      901 gttcctgcgc aacgtcggga tcccgaccta cggccacagc ggcatggcgt ccgacatctt
      961 cgacgtccgc gcccacggca aggacgagcg ggtgtcgatc caggcgacgt tcgagggcga
     1021 gcagtatctc tacgagctgg tcaaggcgct gtccggaggc tgagccgggc accgcgccgc
     1081 cgcggccgcc ccggctacag ctggttctgc tgcgacgagc tgttcggcag gttgctctgg
     1141 gtgatcggct ggttgctctg gttcggcggg ggggcctggt tgatcggccc gttgctcatt
     1201 ggaccctggg gggcctggtt attcatcgga gcctggttgt tcatcggggg agcctggttg
     1261 ttcatcgggc cctggaaatt cgggttgttc atcgggggct ggttgctggt gccggtcgag
     1321 atgaacgcga ccaggtggac cgggcgagcc tgacgaacga ccgtgatggg gagccggccg
     1381 ccctggcgcg cggcgagcgc ctggatcacg tcgtccccgg cgtggaccac gaacggcccg
     1441 acccggacca ggatgtcgcc gacctggatg ccggcgcgcg cggccggcga gttgggctcg
     1501 acgcgcgcga tcagcacgcc gcggtcgtgc gcgacgcgga agtactggcg cagctcgggg
     1561 gtcaggctga tcaccatgac gccgaggcgg ggctggctct cgacgggctc ggtctcggcg
     1621 aagtcttcct cttgatcctg gtccggtggc tgcggcgaac cctgatcctg gaagccgccg
     1681 ggcggtggag cgctcggagc gggatagccc tgcgtcgagg agccctgtgc cgaggagccc
     1741 tgcgccgaat agccgcttgg ctgctggtag ccgccctggg gagaatagcc accctgcgac
     1801 gagtagcttc cctgagggga atagccttgc ggcgcctggt tctgatctcc gcccgagtta
     1861 tcggtccact ggccgcggtt cggggaggcc atcgcgcccg agccgcagcc aagcacgaag
     1921 agcgctgtga gaagctttgg tgcaatccgt gacatgacat ttctcctgag ggccgtactg
     1981 cacagcccac gcgaggggcc tgcatcgccg ataccatcgc gcgctgcgac ggggcggcac
     2041 gggccaccgc cagcggcgca gcgcggggca gatcgtcgat gcgcgtcgac gacacggcgg
     2101 gcatcaggcg cgcagcgctg cgccggcgcc cgaggcaaac tgcgatcgcg gcgtcagcgc
     2161 cggacgatcc ggttctgtcg ccaggccggc ttcgggatca cggccccggt gaccgccgcg
     2221 cgcggatcgg ccgtcctcgg tgcgacctgc gagcaggtcg agctcgcgcg gtacgacagc
     2281 gccgcggcga tccgcgcctc ggccgagtcg ccgagcaggt gcgtgaagtc gtcggcgacg
     2341 gtgcagccct tgaacacgcc gccgggagtg aagccgtcgg cgtagtcccc gaagcccttg
     2401 ttgttcacgc cctggaactg gatcgcgaag aacgtcgtgc cgcagttgtc ctggggcacg
     2461 aagccgtacg gcttgccgca cgtggtctcg ccgatctgga tcacctggac gttgaccccc
     2521 gccaggctgt tcatcaccga ctcgctcgcc gagcaggtcg acgagctggt cagcacgaac
     2581 acgcgcgaca gccccaggga cggcagcggc tggccggcgc cgagccggct gggcacgaag
     2641 cccaggctct ggttgacgaa gccctccggc tggagcggca ggccgctgaa cggatcggtc
     2701 gtcgggtagc ggtcgttgaa ctcgagccgc tcgaacgtct tgccggcggt cgcggtcggc
     2761 ccggcgatca tgtaggcgag ctcggaggcg atgtcgaggt agccgccgcc gttgtagcgc
     2821 atgtcgagga tcagctcggt gaccccggcg gcgcgcagct gggtgaacgc gtcgaacagc
     2881 gccttctcgg cggtcgcgat gtgatcggtg aacaggaagt agccgatctt gtcgttgggc
     2941 gccggcagcg tgcggacgtt cagcaccggc atgatctcga cgctggtcga ggtcagcgtc
     3001 accgtgcgcg tcgtggtcgc gccgaggtcc tggacgacga actggtggct ctcgtggagg
     3061 ttcgacggaa acagccccgc gttgagcgtg tcgacgtcgc tgccgcgctc gacgtcgacg
     3121 ccgtcgatcg acagcaccct ggcgccgcgt ccgagcggag gctggaccgc cgcggcgggc
     3181 gagcccggct cggtgtacgc gatcaagagg ctgcgcgggg gcgagggcgc gagcagcgcc
     3241 cactggatgc cgtagcttgc ctcggtgccc gactgcgaca gcgcctccca gtccgtcgtc
     3301 ttgtagatga agtggaactg gtccttgggc ttgcccgagg ttgtcgtcgc cggcgtcttc
     3361 tcctggtcga agtagtcgac cgcggtcgcg tacttcgtga tgtcgacgct gggcacctcg
     3421 cggtaccaca ggtacagatc gttgatccac gagtggaccc agagcttctc ctccatcacc
     3481 gagccggcga cgtcggtgta gggctgcccg gtcccgggat cgatgccggc gcgcggctgg
     3541 gcgcagcgct cggcgtacac cgactccggg tagatcgcgt tgtcgccgca ggcgggcgcg
     3601 acggccactg ccagcagcaa ccccacacgg cacgccatcg ggctctcggc acggggcgat
     3661 acggctcgat tcatggccgg attcatgtcc ggaaggatag cgggagggct cgccgggcgc
     3721 cgcggaattc gcggtcgggg tgtagcaagg tcgcaatgtt acatgcagca caccgcgatc
     3781 gccggcgcga cctgcccggt cgcggcgggc gcgtcgtcgt cgctaccgca ccgccaccgg
     3841 cgagctcgcc cggatccgcg gctggtcgga ccgcacggtc gcgcacgcgt gcggcgcacg
     3901 tgcggcgcac ggtcatcgct tcgacaccgt cccgggcggt cctgcagcaa ctcctgcgtg
     3961 gagcagctgc ggattgtgcc gggtgtaccg actgacattt cccagcatgg caatcgaacg
     4021 atgtttcaaa gagcgcttga cggcccggcg cggctccggt aaggatgaag tcgggtggct
     4081 ggagtccgcc ttgcgtgcct ggggggcaca tcgcggtcag gagggtgtcg gaggcgtcga
     4141 gagatacgag ggctggggta gtcgagcgtc gcggtgaccg gcatgcggcc atcgcggcat
     4201 ggggttacac gtgcgggggg agacggagct gtggcgacgt actacgcaat ttctgccgtg
     4261 gggcaggcga tcgttgcgct gttgaagaca ggccgatctg ctcccgagct cgcgaatacg
     4321 gaaatcaagc tatttcatat ccgcgatttt cagaaggggc tggaagaggg cgtgtctgtg
     4381 tatctttacc gcgtgactgt cagtggcgcg cgccgcagct tgccaccgac gtacagcccg
     4441 tcggggcggc cgtaccggca gccgctgccc gtggatctgt attacctggt cacgccgtgg
     4501 gccaagactg cggagatgca gcacctcatc ctggcgtggg cgatgcggac catcgaggat
     4561 tcgccgacgt ttcccgcgtc gctgctgaac tccttcgtgc ccgattcctt tcggccggag
     4621 gagagtatcg atctgattca cgacacggtc tcggtgcagg acatgtcgta catctgggag
     4681 atcggaaaag cgaacatgca gacctccgcg acgttcgtcg cgcgggtcgt cgcgatcgac
     4741 tcccgcgtcg agcaacccga tcctggaccg ccggtgcaga cccgcgtgtt cgagtcgcga
     4801 tgaggccagt gccgaggcga gaaatgagga gagatcgatg atcgtccgtc gcacctggag
     4861 tcgagtcgcg ccgctcggcg tgcgcttcgt cgacgatcgc agcggcgacc tcgtggtcga
     4921 caacctcgtc gtcacggcgt ggccggcgcg cgagccgtcc cgacgctcgc cggcgatcgt
     4981 caaccgctcg ggcgtgttca gcctgcatga cctgcccggc ctcggcggtc tggagcacgg
     5041 caccggcgat gacgcgtact ggcgcgccgc gccgcgccgg ccgttcgtcg tcgacgtcga
     5101 ggacctcgcg ctgcgcttcc tggcgttccg cttcaccgtg gagctgccgc tgcgcgggct
     5161 gctcgagctg cgctgcggat cgccgcccgc ggccgtgggg ccggctccgg accgcggcgt
     5221 tccgctgttc accgcgccga cccgcccgac cctgccgggg atggccgtgc tgcgtgccga
     5281 cctgtgggac gccgcggcca acgcgcccgc cgcctgggcc gtggtccagg cctcgatccc
     5341 gagcgccgcg gcgcacgggg tgcctccgat ccgcgcgatc gccgacgacc gcggccagat
     5401 cgcgctgcac atgccgtgtc ccgacgccga ggatctcgac ggcgggtcgt tcgactcgcc
     5461 gagcggtccc gcgccggtcc cgctgaccgc acgcacctgg tcggtcgacc tggcggcgag
     5521 ctacggccac ctgacacccg gtgcgccgat cgtcggtcta ttgcccagca ggggcggcgc
     5581 accgattccg gatctctgtg cggcgatgac ccagccgccg gcaaccttgt ggagcgaccg
     5641 ggcgcgcacg accccgctgc ggcgtgcgtc gctcggcttt ggcagcgaaa ccgtcttgcg
     5701 atcgaccggc agcggcgcgg ggccgcagtc cgtcttgttc ttgacgcctg gctcaccgcc
     5761 ttgaccgcgg gaggaatggg gacatgcccg aatatcttgc accaggagtg tacatcgaag
     5821 aggtctcgtt ccggtcgaag tcgatcgagg gagtgagcac cacgacgacc ggcttcgtcg
     5881 ggcccacccg ggacggaccg gtcgatctcg acaacgagct gatcaccagc ctcggcgact
     5941 tcaagcgggt ctacggggat ggtagccagc tccagttcgg caatgatgtg ctgcacaatt
     6001 acatgtggca cgccgcccgg gcgttcttcg cggaaggcgg caagcagctg tacgtctcgc
     6061 gggtgttccg gcggctggac gccgacagcg cgtcggcccc gccggcgggc acggtcgacc
     6121 gctcgccgtc gcgtagcggc ggggccagcg gaccgtactt cgatggctat gcccgcgcgg
     6181 gcacggccgg ccggctgtcg gacgctaccg cggggacgtc gctggtcgtg cgcgcgcgct
     6241 ggcccggcgc cgccggcaac gtccgggtcc gcttcgggct gcgggtgtcc aagagcgtgc
     6301 tggggttcga ccagcgcagg gatccggtgt cgggggctgc gaccagcgtc gcggtggtct
     6361 cgtcacttgc cgatcgcgac gtcg
//
LOCUS       DASVOR010002182         1949 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_402538,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002182 DASVOR010000000
VERSION     DASVOR010002182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402538"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1198)
                     /locus_tag="VF516_40485"
     CDS             complement(<1..1198)
                     /locus_tag="VF516_40485"
                     /inference="COORDINATES: protein motif:HMM:NF012239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEX8114098.1"
                     /translation="MHKTATRTVPLSRVRNIGIMAHVDAGKTTTTERILYYSGVTSRL
                     GNVDEGSTVTDWMEQEQERGISITAAATTFGWKGHVVNLIDTPGHVDFTMEVERSLRV
                     LDGAIAVFDAVEGVQSQSETVWRQADRYRIPRIAFINKCDREGAEPARVLDEIRTRLG
                     ASPIAVQLPLGIGPTFAGVVDLITMRARTWDVASFGASFTDGPIPDDLAEAASQARDA
                     MVEAIAECDDELMAAYVAGRELSEHAIRTALRRITLGNRGVPALVGAAFKNLGIHNLL
                     DAVLDYLPSPADFAEIRGRDPRDPTGAPTLVRKLGKAGELADTEPLAALAFKVQIDEA
                     GGTLTFVRVYAGCLRVGDAVLNATKGHLEHIGRLVRMFANHREDIKQIEAGMIGAVHD
                     PVPVTAQ"
     gene            complement(1456..1944)
                     /gene="rpsG"
                     /locus_tag="VF516_40490"
     CDS             complement(1456..1944)
                     /gene="rpsG"
                     /locus_tag="VF516_40490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEX8114099.1"
                     /translation="MPRKGEVPKRKVLPDPKYTDAPPDVRRRITKFVNVVMRDGKKAV
                     AEGIVYGALDMVAERAKDDPLKVWDRALSNVRPRVEVKSRRVGGSTYQVPVDVRPDRS
                     LALGMRWLVEYARDRNEKTMMDRLANEIVDAAQNRGNAVKKREDVHKMADANKAFAHY
                     RW"
BASE COUNT          254 a          653 c          719 g          323 t
ORIGIN      
        1 gctgcgcggt caccggcacc gggtcgtgga ccgcgccgat catcccggcc tcgatctgct
       61 tgatgtcctc gcggtggttc gcgaacatcc ggaccagccg gccgatgtgc tcgaggtggc
      121 ccttggtcgc gttgagcacc gcgtcgccga cccgcaggca cccggcgtag acccggacga
      181 aggtcagcgt gccgccggcc tcgtcgatct ggaccttgaa cgcgagcgcg gcgagcggct
      241 ctgtgtcggc gagctcgccg gccttgccga gcttgcggac cagggtcggt gcgccggtcg
      301 gatcgcgcgg atcgcggccg cggatctcgg cgaagtcggc cggcgacggc aggtagtcga
      361 gcaccgcgtc gagcaggttg tggatgccga ggttcttgaa cgcggcgccg accagcgccg
      421 gcacgccgcg gttgccgagc gtgatccggc gcagcgcggt gcggatcgcg tgctcggaca
      481 gctcgcgccc ggcgacatag gcggccatca gctcgtcgtc gcactcggcg atcgcctcga
      541 ccatcgcgtc gcgcgcctgg ctcgccgcct cggcgagatc gtcggggatc ggtccgtcgg
      601 tgaacgacgc gccgaacgac gcgacgtccc aggtccgcgc gcgcatcgtg atcaggtcga
      661 cgacgccggc gaacgtggga ccgatcccga gcggcagctg gaccgcgatc ggcgacgcgc
      721 cgagccgggt ccggatctcg tcgagcacgc gcgcgggctc ggcgccctcg cggtcgcact
      781 tgttgatgaa cgcgatccgc gggatccggt agcggtcggc ctggcgccac accgtctcgc
      841 tctgcgactg gacgccctcg accgcgtcga acaccgcgat cgcgccgtcg agcacgcgca
      901 gcgagcgctc gacctccatg gtgaagtcga cgtggcccgg ggtgtcgatc aggttgacga
      961 cgtggccctt ccagccgaac gtggtcgccg ccgcggtgat cgagatcccg cgctcctgct
     1021 cctgctccat ccagtcggtg accgtcgagc cctcgtcgac gttgccgagc cgggacgtga
     1081 ccccggagta gtacaggatg cgctcggtcg tcgtggtctt gccggcgtcg acgtgcgcca
     1141 tgatcccgat gttgcggacg cgggacagcg ggacggtgcg ggtggcggtc ttgtgcacgg
     1201 cgtgttcgag aggaaccagc gccgcggggc acgaacgaga cgcgaggtcc gcgcccgaga
     1261 gatcggcgcg gcggtcggcg tcatgagctc agcggtttag accacgcatc ccggccggat
     1321 gaccggatgc tgctaccttc cgagccccgc gtgggggata cgcttcgttc gatctcgcct
     1381 cctgggtgcc gtgggtgggg tgtccgtggg gtccgggtga aagtcggatg tccgtgggtc
     1441 gggggggtga aagccctacc agcggtagtg cgcgaacgcc ttgttggcgt cggccatctt
     1501 gtggacgtcc tcgcgcttct tgaccgcatt gccgcggttc tgcgcggcgt cgacgatctc
     1561 gttggcgagc cggtccatca tcgtcttctc gttgcggtcg cgcgcgtact cgaccagcca
     1621 gcgcatgccg agcgcgagcg agcggtcggg gcgaacgtcg accggcacct ggtaggtcga
     1681 gccaccgacg cggcggctct tgacctcgac gcgcgggcgg acgttggaca gcgcgcggtc
     1741 ccagaccttc agcggatcgt ccttggcgcg ctcggcgacc atgtcgaggg cgccgtacac
     1801 gatgccctcg gcgaccgcct tcttgccgtc gcgcatgacc acgttgacga acttggtgat
     1861 ccggcggcgg acgtccggag gcgcgtcggt gtacttgggg tccggcagga ccttgcgctt
     1921 ggggacttca cctttacgag gcatggctg
//
LOCUS       DASVOR010002183         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_257796,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002183 DASVOR010000000
VERSION     DASVOR010002183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_257796"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2006
                     /gene="argJ"
                     /locus_tag="VF516_40495"
     CDS             <1..2006
                     /gene="argJ"
                     /locus_tag="VF516_40495"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEX8114100.1"
                     /translation="PPREARVKLPRGFRFAAVAAGIRPSKPDVALFVSDCDAAGAGVF
                     TINRARSHSVVDAERRLPSDAMRAIVVMSGNANAMTGAAGVEATDAVLAAAARALGVD
                     RAQVASAATGVIGMRLPADKVVAALPRCVAQLGPDPAPVAEAIMTTDTTRKLASRTVE
                     LDGQVVTLTGICKGSGMIAPQLATMIAVIVTDCAVDARTLDAALKQAAPDTFDQLVID
                     GDMSTNDSVFVLANGQAGNPRLTQASGPAFGAFTAALTDLCDELARDIAADGEGATKR
                     LEVRVAGAPSPAVARDIAKAICVSPLVKCAMFGQDPNWGRIMMTIGARCGSQRFDVDP
                     ARAAIAIQGAPVYDAAPVAFDRAQLKARLREPEVVIAVDLRAGAASSKAYGCDLSYDY
                     VKLNADYTSVLFEAPDGGVRKDDRIGNYSPGFKRALLVQALGYISRFRGTRCVIEYGG
                     AAIARDGLRRAFCDDVLLLHTVGLSPIVVYGGASSAELVTILNRAGGHAVGLSGKDAA
                     LLRARKRVTEGASDGGHGGASGELVEVNKVFLESLIGQHYIPVISPVGLGSDGESYHL
                     DGDVVAAGIARALGADKLMYLADVAGIVEGGELVTDLTPATLRGKLDAGVITNGTAGT
                     AAAALAALSGGVRAVHLIDGRIPHNIIAELFTDTGVGTIVRAE"
     gene            2006..>2371
                     /locus_tag="VF516_40500"
     CDS             2006..>2371
                     /locus_tag="VF516_40500"
                     /inference="COORDINATES: protein motif:HMM:NF013483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114101.1"
                     /translation="MADLDDTRARRALIVELIRTRHIATQGDLRDLLRARGHDVTQAT
                     LSRDLAKLGARRVALPGGGTAYEVDGFTARSGGDPQLAAMAHLVLGIREGAAIVVIHT
                     RPGAAQAVAALLDQARLDRI"
BASE COUNT          311 a          841 c          923 g          296 t
ORIGIN      
        1 ggccacctcg cgaggcacgc gtgaagctgc cgcggggctt ccggttcgcg gcggtcgccg
       61 ccgggatccg gccgagcaag ccggacgtcg cgctgttcgt gtcggactgc gacgcggcgg
      121 gcgccggggt gttcacgatc aaccgggcgc ggtcgcactc ggtggtcgac gccgagcgcc
      181 ggctgccgtc ggacgcgatg cgcgcgatcg tggtgatgag cggcaacgcc aacgcgatga
      241 ccggcgcggc cggcgtcgag gcgaccgacg cggtgctggc ggcggcggcc cgcgcgctcg
      301 gcgtcgaccg ggcgcaggtc gcgagcgcgg cgaccggggt gatcgggatg cggctgccgg
      361 cggacaaggt cgtcgcggcg ctgccccgct gcgtcgcgca gctcggcccc gatccggcgc
      421 cggtcgccga ggcgatcatg accaccgaca ccacgcgcaa gctggcgtcg cgcacggtcg
      481 agctcgacgg ccaggtcgtg acgctgaccg ggatctgcaa gggctcgggg atgatcgcgc
      541 cgcagctggc gacgatgatc gcggtgatcg tcaccgactg cgcggtcgac gcgcggacgc
      601 tcgacgccgc gctcaagcag gcggcccccg acacgttcga tcagctggtg atcgacggcg
      661 acatgagcac caacgacagc gtgttcgtgc tggccaacgg ccaggccggc aacccgcggc
      721 tgacccaggc cagcggcccg gcgttcggcg cgttcaccgc ggcgctgacc gatctgtgcg
      781 acgagctggc ccgcgacatc gcggccgacg gcgagggcgc gaccaagcgg ctcgaggtcc
      841 gggtcgcggg cgcgccgtcg ccggcggtcg cgcgcgacat cgccaaggcg atctgcgtgt
      901 cgccgctggt caagtgcgcg atgttcggcc aggacccgaa ctgggggcgg atcatgatga
      961 cgatcggcgc gcgctgcggc tcgcagcggt tcgacgtcga tccggcgcgc gcggcgatcg
     1021 cgatccaggg cgcgccggtg tacgacgcgg cgccggtcgc gttcgaccgc gcgcagctca
     1081 aggcccggct gcgcgagccc gaggtcgtga tcgcggtcga cctgcgcgcc ggcgcggcga
     1141 gcagcaaggc ctacggctgc gacctgtcgt acgactacgt caagctcaac gccgactaca
     1201 cctcggtgct gttcgaggcg cccgacggcg gcgtgcgcaa ggacgaccgg atcggcaact
     1261 actcgccggg gttcaagcgc gcgctgctgg tccaggcgct cggctacatc tcgcggttcc
     1321 gcggcacgcg ctgcgtgatc gagtacggcg gcgcggcgat cgcccgcgac gggttgcgcc
     1381 gggcgttctg cgacgacgtg ctgctgctcc acacggtcgg gctgtcgccg atcgtcgtgt
     1441 acggcggggc gagcagcgcc gagctggtga cgatcctcaa ccgcgcgggc ggccacgcgg
     1501 tcgggctgtc cggcaaggac gcggcgctgc tccgcgcgcg caagcgggtc accgagggcg
     1561 cgagcgacgg cggtcacggc ggcgcgagcg gcgagctggt cgaggtcaac aaggtgttcc
     1621 tggagtcgct gatcggccag cactacatcc cggtgatctc gccggtcggg ctcggcagcg
     1681 acggcgagag ctatcacctc gacggcgacg tggtcgccgc cgggatcgcg cgcgcgctcg
     1741 gcgccgacaa gctgatgtac ctcgccgacg tggcgggcat cgtcgagggc ggcgagctgg
     1801 tcaccgacct gacgccggcg accctgcgcg gcaagctcga cgccggggtg atcaccaacg
     1861 gcacggcggg caccgcggcg gcggcgctcg cggcgctgtc cggcggggtg cgcgcggtgc
     1921 acctgatcga cggccggatc ccccacaaca tcatcgccga gctgttcacc gataccgggg
     1981 tcggcacgat cgtgcgggcg gagtgatggc ggacctggac gacacccggg cgcggcgggc
     2041 gctgatcgtc gagctgatcc ggacccggca catcgcgacc cagggcgatc tgcgcgacct
     2101 gctgcgcgcg cggggccacg acgtcaccca ggccacgctg tcgcgcgatc tcgccaagct
     2161 cggggcgcgg cgggtcgcgc tgccgggcgg cggcaccgcc tacgaggtcg acggcttcac
     2221 cgcgcgctcc ggcggcgatc cgcagctcgc ggcgatggcg cacctggtgc tcgggatccg
     2281 cgagggcgcg gcgatcgtcg tgatccacac ccggcccggc gccgcccagg cggtcgccgc
     2341 gctgctcgac caggcgcggc tcgaccgcat c
//
LOCUS       DASVOR010002184         2387 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_140322,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002184 DASVOR010000000
VERSION     DASVOR010002184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140322"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..612)
                     /locus_tag="VF516_40505"
     CDS             complement(<1..612)
                     /locus_tag="VF516_40505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114102.1"
                     /translation="MNAILAHQVQWVTASPLWPTAHRDRAVMQRPAILRFTSDRFMAE
                     LLAELAAQPEAIGDRLATDSHPSYREPAPGEASTTTDHLKLYQPAHGHFYLVASSLVC
                     RLPGLPDHAVDRGHDEQAGFVIRRLGAAGGELALVGPDTGRTWRAAGPPDRMADGEEL
                     VPLFPLGLGEPGRRRRLFAGLIPVARQEELQAAPIDGAPPATSS"
     gene            complement(609..1328)
                     /locus_tag="VF516_40510"
     CDS             complement(609..1328)
                     /locus_tag="VF516_40510"
                     /inference="COORDINATES: protein motif:HMM:NF016595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HEX8114103.1"
                     /translation="MTDKPPLETIIDERVPAGLGGRWYGVYPALVTEIKGDPDGMGRV
                     KITLPWVADTGDGRYEAWARIATLMAGNSRGSWFIPDKDDEVLIAFEGGDPRRPYVLG
                     GLWNGKDKPPETMDGAGKNARKVLRSRNGVKVTLDDSDGRETLILETPGGQKVMLQDG
                     PGTIELVDANGNSVKLQASGVTIESAAKVSITASQLEMSAGMVTVSAGMSQFSGVVQS
                     PTVLATSVVSSSYTPGAGNIL"
     gene            complement(1338..>2387)
                     /locus_tag="VF516_40515"
     CDS             complement(1338..>2387)
                     /locus_tag="VF516_40515"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HEX8114104.1"
                     /translation="QIFVDGRDKTDLRQGLLELSIVETTAGLYRCEAAFGNYGAVSGS
                     SDFLYFDRKTFDFGKAFKVALNDSVLFDGRITALEARFPSASPPALVVLAEDRLQDLR
                     MTRRTRTFSDASDADVFNRIAEQHGLAKDIRLTGGSHPVLAQVNQSDLAFLRERARAI
                     GAELWIETAGDGRATLKACSRSDRGAGAPIKLGWHNELQEFDVIADLSGQRTSVAATG
                     WDPGAKAQLRYEAGDSDLQGEVGGDDSGPSLLRARLGDRKESIAHAVPLDSREAQARA
                     ASHYQMMARRFVVGHGVARTDPKLRVGSWVDLDGLGPLFNGKYYLSEVRHRFDGSRAL
                     RTEFTAERPGLGRPS"
BASE COUNT          294 a          889 c          845 g          359 t
ORIGIN      
        1 ggaggaggtc gccggcgggg cgccgtcgat cggcgcggcc tggagctcct cctggcgggc
       61 gaccgggatc aggcccgcga acagccgccg gcgccgcccc ggctcgccga gaccgagcgg
      121 gaacagcggg accagctcct cgccgtccgc catccgatcg ggcggccccg cggctcgcca
      181 ggtgcgcccg gtgtcgggcc cgaccagcgc gagctctccg cccgccgcgc cgagccgccg
      241 gatcacgaag ccggcctgct cgtcatggcc gcggtcgacg gcgtgatcgg gcagtccggg
      301 cagccggcac accaggctcg acgccaccag gtagaagtgg ccgtgcgccg gctggtacag
      361 cttcaggtgg tcggtggtgg tcgatgcttc gcccggcgcc ggctcgcggt agctcgggtg
      421 cgagtcggtc gccagccggt cgccgatggc ctcgggctgc gcggcgagct cggcgagcag
      481 ctcggccatg aaccggtcgc tggtgaaccg caggatcgcc gggcgctgca tcaccgcgcg
      541 atcgcggtgc gcggtcggcc acagcggcga cgccgtcacc cactgcacct ggtgcgcgag
      601 gatcgcgttc acaggatgtt ccccgccccc ggcgtgtagc tcgagctgac cacgctggtc
      661 gccagcacgg tcggcgactg cacgacgccc gagaactgcg acatgccggc gctgaccgtc
      721 accatccccg ccgacatctc gagctggctc gcggtgatgc tgaccttggc ggccgactcg
      781 atggtcacgc cgctggcctg cagcttgacc gagttgccat tggcgtcgac cagctcgatc
      841 gtgccggggc cgtcctgcag catgaccttc tggcccccag gtgtctccag gatcagggtc
      901 tcgcggccgt cgctgtcgtc gagcgtcacc ttcaccccgt tgcgcgagcg cagcaccttc
      961 ctggcgttct tcccggcccc gtccatggtc tcggggggct tgtccttgcc gttccacagc
     1021 ccgccgagga cgtacggccg gcgcgggtcg ccgccctcga acgcgatcag gacctcgtcg
     1081 tccttgtccg ggatgaacca gctgccccgg ctgttgccgg ccatcagcgt ggcgatccgc
     1141 gcccacgcct cgtagcggcc gtcgccggtg tcggcgaccc acggcagcgt gatcttgacc
     1201 cggcccatcc cgtcggggtc gcccttgatc tcggtgacca gcgccgggta cacgccgtac
     1261 caccggccgc cgaggccggc cggcacgcgc tcgtcgatga tggtctccag ggggggcttg
     1321 tcggtcacgg gtccctctca gctcggccgg cccaggccgg gtcgctcggc ggtgaactcg
     1381 gtgcgcagcg cccggctgcc gtcgaagcgg tgccggacct cggacaggta atacttgccg
     1441 ttgaacagcg ggccgaggcc gtcgaggtcg acccagctgc cgacccgcag cttcggatcg
     1501 gtgcgcgcca cgccgtgccc gacgacgaac cggcgcgcca tcatctggta gtgcgacgcc
     1561 gcgcgcgcct gcgcctcgcg gctgtcgagc ggcaccgcgt gcgcgatcga ctccttgcga
     1621 tcgccgagcc tggcgcgcaa gaggctcggg ccgctgtcat cgccgccgac ctcgccctgg
     1681 aggtcggagt cgccggcctc gtagcggagc tgcgccttgg cgccgggatc ccagccggtc
     1741 gcggcgacgc tcgtccgctg cccggacaga tcggcgatca cgtcgaactc ctggagctcg
     1801 ttgtgccagc ccagcttgat cggcgcgccg gcgccgcgat cgctccggct gcacgccttc
     1861 agcgtcgcgc gcccgtcgcc tgcggtctcg atccacagct cggcgccgat cgcgcgcgcc
     1921 cgctcgcgca ggaacgcgag atcgctctgg ttgacctgcg cgagcaccgg gtgcgagccg
     1981 ccggtcagcc ggatgtcctt ggccagcccg tgctgctcgg cgatccggtt gaacacgtcg
     2041 gcgtcgctgg cgtcgctgaa cgtccgcgtc cgccgggtca tccgcaggtc ctggagccgg
     2101 tcctcggcga gcacgaccag ggcgggcggg ctggccgacg ggaaccgggc ctcgagcgcg
     2161 gtgatccgcc cgtcgaacag cacgctgtcg ttcagcgcga ccttgaacgc cttgccgaag
     2221 tcgaacgtct tgcggtcgaa gtacaggaag tcgctgctgc ccgagaccgc gccgtagttg
     2281 ccgaacgccg cctcgcagcg gtacagcccg gcggtggtct cgacgatcga gagctcgagc
     2341 agcccctggc gcaggtcggt cttgtcccgg ccgtccacga agatctg
//
LOCUS       DASVOR010002185         2939 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_431930,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002185 DASVOR010000000
VERSION     DASVOR010002185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431930"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1274)
                     /gene="pcnB"
                     /locus_tag="VF516_40520"
     CDS             complement(<1..1274)
                     /gene="pcnB"
                     /locus_tag="VF516_40520"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0006378 - mRNA polyadenylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEX8114105.1"
                     /translation="MPSLDRAAIDPDADRVVRKLTRAGHKAYLVGGCVRDLLVARKPK
                     DFDVATSATPNEIKATFRNCRIIGRRFRLAHVFFGAKIIETSTFRANPRDEDDHDLLI
                     RRDNVFGTETEDARRRDFTINGLFYDVEREEVIDHVGGLADLDARLIRTIGDPDIRFQ
                     EDPVRILRAIKFAARLDFGFEPATWRALLRWRGEISKCAPPRLLEEVHRLMRGGAARR
                     SFELMVETGVLAVLSPYLAGLLEGEGAPATAPPIAGVAPRATIPQIEYDEEDPAEHDP
                     DDPPEEDGENGEAEDTEAEGEGEIDGEGDEIDLDDEERAWHRVWADEPAVRPAAAAPV
                     RLSFMSSDRELVRRRTLAWAALPWIDKAVADGRDPSNAVAFGALLAPFLPEEVRGGEL
                     AAAVHELAHPLIGQLHVTRRDSERMRYLLIAQR"
     gene            complement(1404..>2939)
                     /locus_tag="VF516_40525"
     CDS             complement(1404..>2939)
                     /locus_tag="VF516_40525"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEX8114106.1"
                     /translation="IVHEVSPPAGRPAGAPYRVELTSTADPGARIEVMIDRKPAWSNI
                     VTGLDLDRRTRWGELYALDDSSVREVEDHQWLRTAYRFAHVQDKGDVPRIDRALEYAT
                     IDREQIYVLTLFGSPGEIEQLEETVVPTLRVATQTGLPLVPQTGHLSSRKYPNPVARA
                     FDSTVMVVVADLIDGRLRARGGGSGVIVGKDGSILTNYHVIHDRNGRLHDVFVIGRFS
                     EQDKAPQLHCAGKPSRSKLQRELDLALIKCDMDLDGRSWTPARSGAAWPTLLLAHTAD
                     VKTGQRLWALGYPDVGGGGLTLSEGEALGWSGVDGAAGNDFLKTDAAIRHGNSGGPVV
                     GDDGRLIGIASAVRTKLSAAGDVVEVVQAGGRVRPIAAASDLLAIAAAGWTPREGATD
                     VELQPSAIEVPAEGVQIQSRVLDVANEAPVREALVMVLRPGVNAATLDVNRLDDQVLA
                     YGRSNAQGEVVLKQPVPVPGTYSLMVVARGYEPLIGEGELTLAADTPAVFDPWGKLLL
                     RAR"
BASE COUNT          373 a         1104 c         1033 g          429 t
ORIGIN      
        1 cgctgcgcga tcagcagata gcgcatccgc tcgctgtcgc gccgggtgac gtggagctgg
       61 ccgatcagcg ggtgggcgag ctcgtggacc gcggccgcga gctcgccgcc gcgcacctcc
      121 tcgggcagga acggggccag cagggcgccg aacgccaccg cgttcgacgg atcgcggccg
      181 tcggcgaccg ccttgtcgat ccacggcagc gccgcccacg ccagcgtccg gcgccggacc
      241 agctcgcggt ccgacgacat gaacgacagc cggaccggag cggcggcggc cgggcgcacg
      301 gccggctcgt cggcccacac ccggtgccag gcgcgctcct cgtcgtcgag gtcgatctcg
      361 tcgccctcgc catcgatctc gccctcgccc tcggcctccg tgtcctcggc ctcaccgttc
      421 tcgccgtcct cctccggcgg atcgtcggga tcgtgctccg cgggatcctc ctcgtcgtac
      481 tcgatctgcg ggatcgtcgc gcgcggtgcc acgccggcga tcggcggcgc ggtcgcgggc
      541 gcgccctcgc cctccagcag gccggcgagg tacggcgaca gcaccgcgag cacgccggtc
      601 tcgaccatga gctcgaacga ccgccgggcc gcgccgccgc gcatcaggcg gtggacctcc
      661 tcgagcagcc ggggcggcgc gcacttgctg atctcaccgc gccagcgcag cagcgcgcgc
      721 caggtcgccg gctcgaaccc gaagtcgagc cgggcggcga acttgatcgc gcgcaggatc
      781 cggaccgggt cctcctggaa ccggatgtcc ggatcgccga tcgtccggat cagcctggcg
      841 tcgagatcgg cgagcccgcc gacgtggtcg atcacctcct cgcgctcgac gtcgtagaac
      901 aggccgttga tcgtgaagtc gcggcggcgc gcgtcctcgg tctcggtccc gaacacgttg
      961 tcgcggcgga tcagcaggtc gtgatcgtcc tcgtcgcgcg ggttggcgcg gaacgtcgag
     1021 gtctcgatga tcttcgcacc gaagaacacg tgggcgagcc ggaaccggcg gccgatgatc
     1081 cggcagttgc ggaacgtcgc cttgatctcg ttgggcgtcg cgctggtcgc gacgtcgaag
     1141 tccttcggtt tccgcgcgac cagcagatcg cggacacatc cgcccacgag gtacgccttg
     1201 tggcctgcgc gggtgagctt gcggaccacg cgatcggcgt cgggatcgat cgcagctcgg
     1261 tcaagcgagg gcatcttcgc gagtcagccg taccatggcg cgtcgatcgc gggcaagtat
     1321 gcgatcctct tgtgatgttt tgagcacccc gggacggccg gatcggccgg cccagccggt
     1381 cgcatctccc gaccgcgcgc ggctcagcgc gcccgcagca gcagcttgcc ccacgggtcg
     1441 aacaccgccg gggtgtcggc cgcgagcgtc agctcgccct cgccgatcag cggctcgtag
     1501 ccgcgggcga cgaccatcag cgagtaggtc cccggcaccg ggaccggctg cttgagcacg
     1561 acctcacctt gcgcgttgga ccggccgtag gccagcacct ggtcgtcgag ccggttgacg
     1621 tcgagcgtcg ccgcgttgac cccgggccgc agcaccatga ccagcgcctc gcgcaccggc
     1681 gcctcgttcg cgacgtcgag cacgcggctc tggatctgca cgccctcggc gggcacctcg
     1741 atcgccgacg gctggagctc gacgtcggtt gcgccctcgc gcggcgtcca ccccgcggcg
     1801 gcgatcgcca gcaggtcgct cgcggcggcg atcggacgga cccggccgcc ggcctggacc
     1861 acctcgacga cgtcgcccgc ggcgctcagc ttggtgcgca ccgccgacgc gatgccgatc
     1921 agccggccgt cgtcgccgac caccgggccg ccggagttgc cgtgccggat cgccgcgtcg
     1981 gtcttgagga agtcgttgcc ggcggcgccg tcgacgccgc tccaccccag cgcctcgccc
     2041 tcgctgagcg tcagcccgcc gccgccgacg tcggggtagc ccagcgccca cagccgctgg
     2101 ccggtcttga cgtcggcggt atgggccagc agcagcgtcg gccacgccgc gccgctccgc
     2161 gccggggtcc agctccggcc gtcgagatcc atgtcgcact tgatcagcgc gaggtcgagc
     2221 tcgcgttgca gcttgctccg gctcggcttg ccggcgcagt gcagctgcgg cgccttgtcc
     2281 tgctcgctga accggccgat cacgaacacg tcgtgcagcc ggccgttgcg gtcgtggatc
     2341 acgtggtagt tggtgaggat cgagccgtcc ttgccgacga tgacgcccga gccgccgccg
     2401 cgcgcgcgca gccggccgtc gatcaggtcg gcgaccacca ccatcaccgt cgagtcgaac
     2461 gcgcgggcca ccgggttggg gtacttgcgc gacgacaggt ggccggtctg cggcacgagc
     2521 ggcaggccgg tctgggtcgc gacccgcagc gtcggcacca ccgtctcctc gagctgctcg
     2581 atctcgcccg gcgagccgaa cagcgtcagc acgtagatct gctcgcggtc gatcgtcgcg
     2641 tactccagcg cgcggtcgat ccgcggcacg tcgcccttgt cctggacgtg ggcgaaccgg
     2701 tacgcggtgc gcagccactg gtggtcctcg acctcgcgca ccgagctgtc gtcgagcgcg
     2761 tacagctcgc cccaccgggt ccggcggtcg aggtcgagcc cggtgacgat gttgctccac
     2821 gccggcttcc tgtcgatcat cacctcgatc cgcgcgccgg ggtccgccgt cgaggtcagc
     2881 tcgacgcggt acggcgcacc ggccggccgg ccggccggcg gcgacacctc gtggacgat
//
LOCUS       DASVOR010002186         2586 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_374209,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002186 DASVOR010000000
VERSION     DASVOR010002186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374209"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1393)
                     /locus_tag="VF516_40530"
     CDS             complement(<1..1393)
                     /locus_tag="VF516_40530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007732506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEX8114107.1"
                     /translation="MLLRDKLYIDGQWATPSTQETIDVHSAGTGEVMGKVPAGGEADI
                     AAAVRAARAAFDSWSQLPVAQRADYLAKISAGLKQRAELLARTIAQEVGMPIKLAGRI
                     QAGLPMATFANYARIAGEFAFEEKVGNSLVVRDPVGVVGAITPWNYPLHQIALKVAPA
                     LAAGCTVVLKPSEIAPFNAFLLAEVVHEVGLPKGVFNLVTGFGPVVGEALVKHPEVDM
                     ISFTGSTRAGRRISEVAAQMVKRVTLELGGKSASIILDDADLPAAVKGTVTNCYLNSG
                     QTCTAFTRMLVPEARYAEARALAAEAAAAFVPGDPLSESSRLGPLSSQMQLERVRGYI
                     RKGLEEGAELVTGGAEPPEGVAAGGYFVRPTVFGKVDPKSTIAQEEIFGPVLSIITYK
                     DEDDAVRIANDTIYGLAGGVWSKDEARAQRVARRMRAGQIDINGGPFNMNAPFGGFKQ
                     SGHGREAGTYGLEE"
     gene            1538..>2586
                     /locus_tag="VF516_40535"
     CDS             1538..>2586
                     /locus_tag="VF516_40535"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEX8114108.1"
                     /translation="MARKGKAVVARELGKPVLVEEVAFDSPGRGEVMVKIGAVGVCHS
                     DLSGTNGTIALPLPLILGHEAAGTVVEVGEGVTELAVGDAVVANWIYMCGRCHYCNVG
                     RPVLCDQQGKALSTMFDGTLRTRDAAGNPLNVFSGVGVMAEYAVVHERNLVRYDAKVP
                     ADRAALVGCAVTTGVGAAINTARVAPGSICGVWGTGGVGLNVIQGCAIAGAGRIIAID
                     TSEAKLEFARTFGATDAIKAAPGEDLTKQLKKLTGGGLDYAFECVGSGDVVAAAYKAI
                     RRGGMAVAVGVAPASASTTVVTRTLPFEEKTLTGSYFGSARPREDFPKMLSLYAAGKL
                     KLDELITRRYKIDEAP"
BASE COUNT          364 a          944 c          896 g          382 t
ORIGIN      
        1 actcttccag cccgtacgtg ccggcctcgc ggccgtggcc cgactgcttg aagccgccga
       61 acggcgcgtt catgttgaac ggcccgccgt tgatgtcgat ctgcccggcg cgcatccgcc
      121 gcgcgacccg ctgcgcgcgc gcctcgtcct tcgaccacac gccgccggcc agcccgtaga
      181 tcgtgtcgtt ggcgatccgg accgcgtcgt cctcgtcctt gtacgtgatg atcgacagca
      241 ccgggccgaa gatctcctcc tgcgcgatcg tcgacttcgg gtcgaccttg ccgaacaccg
      301 tcggccggac aaagtagccg ccggccgcga ccccctcggg tggctcggcg ccgccggtca
      361 ccagctcggc gccctcctcg agccccttgc ggatgtagcc gcgcacgcgc tcgagctgca
      421 tctgcgacga cagcggaccg agccgcgagc tctccgacag cggatcgcct ggcacgaacg
      481 ccgcggccgc ctcggcggcg agcgccctgg cctcggcgta gcgcgcctcg ggcaccagca
      541 tgcgggtgaa cgccgtgcag gtctggccgg agttgaggta gcagttggtc accgtgccct
      601 tcaccgcggc cggcaggtcg gcgtcgtcga ggatgatcga cgcggacttg ccgccgagct
      661 cgagcgtcac gcgcttgacc atctgcgccg cgacctcgga gatccgccgg cccgcgcggg
      721 tcgatccggt gaacgagatc atgtcgacct cggggtgctt gaccagcgcc tcgccgacca
      781 ccggcccgaa cccggtgacc aggttgaaca cgcccttcgg cagcccgacc tcgtgcacca
      841 cctcggcgag gaggaacgcg ttgaacggcg cgatctccga cggcttgagc accaccgtgc
      901 agccggcggc gagcgcgggg gcgaccttga gcgcgatctg gtgcagcggg tagttccacg
      961 gcgtgatcgc gccgaccacg ccgaccgggt cgcgcaccac cagcgagttg ccgaccttct
     1021 cctcgaaggc gaactcgccg gcgatcctgg cgtagttggc gaacgtcgcc attggcagcc
     1081 cggcctgaat ccggccggcg agcttgatcg gcatgccgac ctcctgcgcg atcgtcctgg
     1141 cgagcagctc ggcgcgctgc ttgagccccg ccgagatctt cgccaggtag tcggcgcgct
     1201 gggcgaccgg cagctggctc cagctgtcga acgccgcgcg ggcggcgcgg accgcggcgg
     1261 cgatgtcggc ctcgccgccg gcgggcacct tgcccatgac ctcgccggtg ccggcgctgt
     1321 gcacgtcgat ggtctcctgc gtgctgggag tggcccactg gccgtcgatg tagagcttgt
     1381 cgcggagcag catggtgttc ccgggaatga gggagcgcgg atgcgcgggg ggtgtagcgc
     1441 ggggagttgt agcgcgggga gtggttgcgc gcggagtata cgggaatttc gccaccaagc
     1501 tgttgcccgg cgatccgcct tgaaggaggc ttcgcccatg gcacgcaagg gcaaagcagt
     1561 cgtcgctcga gagctcggta agccggtcct cgtcgaggag gtcgcgttcg attcgccggg
     1621 gcggggcgag gtgatggtca agatcggcgc ggtcggcgtc tgtcactccg acctgtccgg
     1681 caccaacggc accatcgcgc tgccgctccc gctgatcctc ggccacgagg ccgcgggcac
     1741 cgtcgtcgag gtcggcgagg gcgtcaccga gctcgcggtc ggcgacgccg tggtcgccaa
     1801 ctggatctac atgtgcggcc gctgccacta ctgcaacgtc ggccgcccgg tgctgtgcga
     1861 ccagcagggc aaggccctgt cgacgatgtt cgacggcacc ctgcgcaccc gcgacgccgc
     1921 cggcaacccg ctcaacgtgt tctccggcgt cggcgtgatg gccgagtacg ccgtcgtcca
     1981 cgagcgcaac ctggtcaggt acgacgccaa ggtcccggcc gatcgcgccg ccctcgtcgg
     2041 ctgcgcggtc accaccggcg tcggcgccgc gatcaacacc gcgcgggtcg cgcccggctc
     2101 gatctgcggc gtgtggggca ccggcggcgt cggcctcaac gtcatccagg gctgcgcgat
     2161 cgccggcgcc ggccggatca tcgcgatcga caccagcgag gccaagctcg agttcgcccg
     2221 gacgttcggc gcgaccgatg caatcaaggc cgcgcccggc gaggacctca ccaagcagct
     2281 caagaagctc accggcggcg gcctcgacta cgcgttcgag tgcgtcggct ccggcgacgt
     2341 cgtcgccgcc gcctacaagg cgatccgccg cggcggcatg gccgtcgccg tcggcgtcgc
     2401 gccggccagc gcctcgacca ccgtcgtcac ccgcaccctg ccgttcgagg agaagaccct
     2461 gaccggcagc tacttcggct cggcgcgacc gcgcgaggac ttcccgaaga tgctctcgct
     2521 ctacgccgcc ggcaagctca agctcgacga gctgatcacc cgccgctaca agatcgacga
     2581 ggcgcc
//
LOCUS       DASVOR010002187         1759 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_285427,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002187 DASVOR010000000
VERSION     DASVOR010002187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285427"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VF516_40540"
     CDS             complement(<1..597)
                     /locus_tag="VF516_40540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114109.1"
                     /translation="MAIPGAAPARLGRYELLARLAAGGMGEIFLARLEGAAGFEKLFV
                     IKRILPHLADDPRFRAMLIAEARIASMMTHANICQVYELGETDGQLYIAMEYLEGVTL
                     LPLLRKLSKEGGALDFGFVAGVIQQATDALHYAHELRDRGGDMLGIVHRDVTPTNLFL
                     TDTGMVKILDFGIAKVKDVSANTQAGMVKGKYAYMAPEQ"
     gene            complement(718..1434)
                     /locus_tag="VF516_40545"
     CDS             complement(718..1434)
                     /locus_tag="VF516_40545"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 domain-containing protein"
                     /protein_id="HEX8114110.1"
                     /translation="MAESLLALRDRREQVISRLSDGYARDLIDVDELERRLDLAHGAR
                     SLAELDPLVADLGDPEPRTASTALVVAGPTAIDDPERAATKGLRVIMSAIERRGRWTV
                     PRSLDLRVLWGHAELDFREASLGPGVTTIHVRVFMGNFEVVLPPNLAIEVDVSSFAGS
                     VTERHRVPPDDDPGRPQIRIVGTVRFGNLEITTRLPGESARDAYRRERRARKELRRSE
                     RDAYRLERRERKLLPPGSGS"
     gene            1433..>1759
                     /locus_tag="VF516_40550"
     CDS             1433..>1759
                     /locus_tag="VF516_40550"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEX8114111.1"
                     /translation="MTRGIHRRVRFELHCHSTCSDGTEPPEQVAARAAERRVAVFALT
                     DHDTCAGSGVTVPGARTIRAVELSCDDGGRTVHVLAFDRGGAWSVLEDRLAAVREARR
                     NRLRVMA"
BASE COUNT          259 a          627 c          622 g          251 t
ORIGIN      
        1 ctgctcgggc gccatgtacg cgtacttgcc cttcaccatg ccggcctggg tgttggccga
       61 gacgtccttg accttggcga tcccgaagtc gaggatcttg accatgccgg tgtcggtgag
      121 gaacaggttg gtcggcgtga catcgcggtg gacgatgccg agcatgtcgc cgccgcggtc
      181 gcgcagctcg tgggcgtagt gcagcgcgtc ggtcgcctgc tggatcacgc cggccacgaa
      241 gccgaagtcg agcgcgccgc cctccttcga cagcttgcgg agcagcggca gcagcgtgac
      301 gccctccagg tactccatcg cgatgtagag ctggccgtcg gtctcgccca gctcgtagac
      361 ctgacagatg ttggcgtgcg tcatcatcga cgcgatccgc gcctcggcga tcaacatcgc
      421 gcggaaccgc gggtcgtctg ccaggtgcgg caggatccgt ttgatcacaa acagtttctc
      481 gaacccggcc gccccctcca gacgggccag gaagatctct cccatgccgc ctgccgcgag
      541 gcgtgcgagc agctcgtagc gaccgaggcg agccggcgca gcgcccggaa ttgccatcga
      601 gccagagctt agcagagttg tggaacgatc ctgcgcagtc caacccctca ggatcccacg
      661 ggccgccggc ccgggccaag ggacaagcga aggcggcgac acggtgacgg aacggtgtca
      721 ggaaccgctg cccggcggca gcagcttgcg ctcgcggcgc tcgagccggt acgcgtcgcg
      781 ctccgaccgg cgcagctcct tgcgcgcccg ccgctcccgc cggtaggcat cgcgcgcgga
      841 ctcgcccggc aagcgcgtcg tgatctcgag gttgccgaac cggacggtgc ccacgatccg
      901 gatctgcggc cgccccggat cgtcgtccgg cggcacgcga tggcgctcgg tgaccgagcc
      961 ggcaaacgac gaaacgtcga cctcgatcgc gaggttgggg ggcagcacca cctcgaaatt
     1021 gcccatgaac acgcggacgt ggatcgtcgt gaccccggga ccgagcgacg cctcgcggaa
     1081 gtcgagctcg gcgtggcccc acagcacgcg caggtcgagc gagcgcggca ccgtccaccg
     1141 gccgcggcgc tcgatcgcgc tcatgatcac gcgcaacccc ttggtggcgg cgcgctcggg
     1201 gtcgtcgatc gcggtcggac cggcgaccac cagcgcggtg gacgcggtgc gcggctccgg
     1261 gtcaccgagg tcggcgacca gcggatcgag ctcggccagg ctgcgcgcgc cgtgcgcgag
     1321 atcgagccgg cgctccagct cgtcgacgtc gatcaggtcg cgcgcgtagc catcggacag
     1381 tcgggagatc acctgctccc tgcgatcgcg cagcgccaag aggctctcgg ccatgacccg
     1441 aggtatacac cgccgtgtgc ggttcgagct gcactgtcac tcgacttgct ccgacggcac
     1501 cgagccgccg gagcaggtcg ccgcccgcgc cgccgagcgc cgggtcgccg tgttcgcgct
     1561 gaccgatcac gacacctgcg ccggctcggg cgtcaccgtg cccggcgccc ggacgatccg
     1621 cgcggtcgag ctgtcgtgcg acgacggcgg tcgcaccgtc cacgtcctgg cgttcgaccg
     1681 cggcggcgcg tggtccgtgc tggaggaccg gctcgcggcg gtgcgcgagg ctcgccgcaa
     1741 ccggctgcgc gtgatggcg
//
LOCUS       DASVOR010002188         3525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_198922,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002188 DASVOR010000000
VERSION     DASVOR010002188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198922"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..857
                     /locus_tag="VF516_40555"
     CDS             54..857
                     /locus_tag="VF516_40555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114112.1"
                     /translation="MHARAWIIGAAAIWAGWAGWAGLASTAAADPAPRPVDVKAMKAQ
                     GVVLQDAQGGVYVVFRGEDPKVFYGPNSKAVYEQVIVGSSSDGEGRWEFNTWAPRKPG
                     NGLASLEHKKDGSYHKSCGDTDDTGLTELTGDKAHAVLDRTPFLSMGIVRVPHLLARD
                     DSGVYYYVDRIAKVYGGKGYRVFVGRKGALKQLPLVDVASDSAGDVFSTKSGDLRLVR
                     NKEDDKQKLTWVKGGKRTELVFLDTDVNAVVIFKDLGLYGFLGTLCDDV"
     gene            908..1669
                     /locus_tag="VF516_40560"
     CDS             908..1669
                     /locus_tag="VF516_40560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8114113.1"
                     /translation="MKLNGSVALITGGASGLGAATARRLIAGGAKVVLVDRDDARGQA
                     LAGEIGATFAKADVTDTGQVEAAVATAAQLGPLRIAVSCAGVGWAARTLDKTGKPHDL
                     ELFKTVIGVNLIGTFNVMRLAAAAIARTEALEHGERGVIVNTASVAAFDGQIGQIAYA
                     ASKAGVAGMTLPAARDLAPAGIRVVTIAPGIFDTPMLGTLPEDRRAALAADVVFPKRL
                     GDPAEYGALVAAIVETGYLNAETIRLDGGIRMPPK"
     gene            complement(1697..>3525)
                     /locus_tag="VF516_40565"
     CDS             complement(1697..>3525)
                     /locus_tag="VF516_40565"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEX8114114.1"
                     /translation="GYLQRRPHGPRRSEARLHLAEAKEQAGAPVAEPIALYRQIEIDD
                     PLAPWATRAHDRIVALARAHRLDTKQLEAETAAEHITRGMVLFDAMRNPESEAAFDDA
                     LRDPKISTANRCVAAYHKAQSRFKARDRKGAAPMFDEAAAACKDAHNADLQIKSNYQA
                     GRSYAFIGEHDTAIQRYQAAQTIDPKHSYADDALLREAEEWTSLGDAKQACALLSALP
                     AKFPDSDNVAEAMWRLGWHAWRDQKYDEAITWWQKQIALVPRDDSYYGEGEAQYWLGR
                     AFAAQHRVQDAIASWQAAVRIAPAAYYALLSLNRLREIDAKAYAATIAEISTDPKDYD
                     PGAPAFAFRARPEWSAPGFQRAMELLRLGLGVPAERELKKLGLTAPGDRKRVDDPDRS
                     EKLWAMAFLYDRAGRHATSIWPTRWHILDYRAQWPIGANRARWQIAYPKAYWELLTRH
                     ATANNVPIAMQIAIVREESGFDPLDESYANAIGLTQMIPPTAKDFAKGTGIDPTRENL
                     RDPEKNVTIGGRFLASLYKRWDRFTLLVPPSYNAGPAGVKKMLRVRGTWDADEFVEGI
                     VDDQARNYTKRVLGTYFTYSWLYDHDVPVIPNAIPKALLPKS"
BASE COUNT          483 a         1201 c         1305 g          536 t
ORIGIN      
        1 cgagcggcgc gcgccgggat gaaacggccc ggtcccggcg tatgatgcgg acgatgcacg
       61 cacgagcatg gatcatcggc gccgcggcga tctgggccgg ctgggccggc tgggcggggc
      121 tggcgtcgac cgccgccgcc gaccccgccc ccaggccggt cgacgtcaag gcgatgaagg
      181 cgcagggcgt ggtcctgcag gacgcgcagg gcggggtcta cgtcgtgttc cgcggcgaag
      241 accccaaggt gttctacggg ccgaacagca aggcggtgta cgagcaggtc atcgtcggct
      301 cgtcgagcga cggcgagggc cgctgggagt tcaacacctg ggcgccgcgg aagcccggca
      361 acggcctcgc ctcgctcgag cacaagaagg acggcagcta ccacaagagc tgcggcgaca
      421 cggacgacac cgggctgacc gagctgaccg gcgacaaggc gcacgcggtg ctcgacagga
      481 ccccgttcct gtcgatgggc atcgtccgcg ttccgcacct gctcgcgcgc gacgacagcg
      541 gcgtgtacta ctacgtcgac cggatcgcga aggtctacgg cggcaagggc tatcgcgtgt
      601 tcgtcggtcg caagggcgcg ctgaagcagc tgccgctggt cgacgtggcg agcgacagcg
      661 cgggcgacgt gttctcgacc aagagcggcg acctccggct ggtgcgcaac aaggaggacg
      721 acaagcagaa gctgacctgg gtcaagggcg ggaagcgcac cgagctggtg ttcctcgaca
      781 ccgacgtcaa cgcggtcgtg atcttcaagg acctcggcct ctacggcttc ctcggcacgc
      841 tgtgcgacga cgtgtgacgc cgccccggta gctttgcccg ccgcgaatcg cgtgtatggt
      901 cgcggccatg aagctcaacg gatcggttgc tctcatcact ggaggcgcct cggggctcgg
      961 tgcggcgacg gcgcgccgcc tgatcgccgg cggcgccaag gtcgtgctgg tcgatcgcga
     1021 cgacgcgcgc ggccaggcgc tcgcgggcga gatcggcgcg acgttcgcca aggccgacgt
     1081 cacggacacc gggcaggtcg aggccgcggt cgccaccgcc gcccagctcg ggccgctgcg
     1141 gatcgcggtg tcgtgcgccg gcgtcggctg ggcggcgcgc acgctcgaca agaccggcaa
     1201 gccgcacgac ctggagctgt tcaagaccgt catcggcgtc aacctgatcg gcacgttcaa
     1261 cgtgatgcgg ctggcggcgg cggcgatcgc gaggaccgag gcgctcgagc atggcgagcg
     1321 cggggtgatc gtcaacaccg cctcggtcgc ggcgttcgac ggtcagatcg gccagatcgc
     1381 gtacgcggcc tccaaggccg gcgtggcggg catgacgctg ccggcggcgc gcgacctggc
     1441 cccggccggg atccgcgtgg tgacgatcgc cccggggatc ttcgacaccc cgatgctcgg
     1501 cacgctgccc gaggaccggc gcgctgccct cgcggccgac gtcgtgttcc ccaagcggct
     1561 gggcgacccg gccgagtacg gcgcgctggt cgctgcgatc gtcgagaccg gctacctcaa
     1621 cgccgagacg atccggctcg atggcggcat ccgcatgccg cccaagtgag tccgagccgc
     1681 cggccgcacg ggccgctcag ctcttgggca gcagcgcctt ggggatcgcg ttggggatca
     1741 ccggcacgtc gtggtcgtag agccagctgt acgtgaagta cgtgccgagc acgcgcttgg
     1801 tgtagttgcg cgcctgatcg tcgacgatgc cctcgacgaa ctcgtcggcg tcccacgtgc
     1861 cgcgcacccg cagcatcttc ttcacgccgg cgggaccggc gttgtacgac ggcgggacca
     1921 gcagcgtgaa gcgatcccag cgcttgtaca gcgaggccag gaaccggccg ccgatcgtga
     1981 cgttcttctc gggatcgcgc aggttctcgc gggtcggatc gatgccggtg cccttggcga
     2041 agtccttggc ggtcggcggg atcatctggg tcaggccgat cgcgttggca tagctctcgt
     2101 cgagcggatc gaacccgctc tcctcgcgca cgatcgcgat ctgcatcgcg atcggcacgt
     2161 tgttggcggt ggcgtggcgg gtgagcagct cccagtacgc cttggggtag gcgatctgcc
     2221 agcgcgcgcg gttggcgccg atcggccact gcgcgcggta gtcgaggatg tgccagcggg
     2281 tcggccagat cgacgtcgcg tggcgcccgg cgcgatcgta gaggaacgcc atcgcccaca
     2341 gcttctcgct cctgtcggga tcgtcgaccc gcttgcggtc gcccggcgcg gtgagcccga
     2401 gcttcttgag ctcgcgttcg gcggggacgc cgaggccgag ccggagcagc tccatcgcgc
     2461 gctggaaccc gggggcgctc cactcggggc gcgcccggaa cgcgaacgcc ggcgcgcccg
     2521 gatcgtagtc cttgggatcg gtcgagatct cggcgatcgt cgcggcgtag gccttggcat
     2581 cgatctcgcg cagccggttg agcgacagca gcgcgtagta cgcggccggc gcgatccgca
     2641 ccgcggcctg ccacgacgcg atcgcgtcct ggacccggtg ctgcgccgcg aacgcgcggc
     2701 cgagccagta ctgggcctcg ccctcgccgt agtagctgtc gtcgcgcggg accagcgcga
     2761 tctgcttctg ccaccaggtg atcgcctcgt cgtacttctg gtcgcgccag gcgtgccagc
     2821 cgagccgcca catcgcctcg gcgacgttgt cgctgtcggg gaacttcgca ggcagcgccg
     2881 acagcagggc acaggcctgc ttggcgtcgc ccagcgacgt ccactcctcg gcctcgcgca
     2941 gcagcgcgtc gtcggcgtag ctgtgcttcg gatcgatggt ctgggccgcc tggtagcgct
     3001 ggatcgcggt gtcgtgctcg ccgatgaacg cgtacgaccg gccggcctgg tagttgctct
     3061 tgatctggag atcggcgttg tgggcgtcct tgcaggcggc cgcggcctcg tcgaacatcg
     3121 gcgccgcgcc cttgcgatcg cgcgccttga accggctctg cgccttgtgg tacgcggcga
     3181 cgcagcggtt ggcggtcgag atcttcggat cgcggagcgc gtcgtcgaac gcggcctccg
     3241 actcggggtt gcgcatcgca tcgaacagca ccatgccgcg ggtgatgtgc tcggccgcgg
     3301 tctcggcctc gagctgcttg gtgtcgagcc ggtgggccct ggcgagcgcg acgatccggt
     3361 cgtgcgcgcg ggtcgcccac ggcgccagcg ggtcgtcgat ctcgatctgg cggtacagcg
     3421 cgatcggctc ggcgaccggc gcgccggcct gctccttggc ctccgcgagg tggagccgcg
     3481 cctcggagcg ccgcgggccg tgcggccggc gctgcaggta gcccg
//
LOCUS       DASVOR010002189         3789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_315980,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002189 DASVOR010000000
VERSION     DASVOR010002189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315980"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VF516_40570"
     CDS             complement(<1..941)
                     /locus_tag="VF516_40570"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114115.1"
                     /translation="MVGQYQLGELLGKGGMGQVHAARHRSGRAVVIKRVRNTLSSNQL
                     LAARLADEARLLRAVSHPNVVRVLDHGTGHDGRPFLVMDRAHGTPLCDMIAACGPQPR
                     ERVAAIASQLFAGLAAIHAAGIVHADLTSGNVLVDKVDIVTIIDFGLACPVSASPGTT
                     DDVAGTPAYMAPEMIAGEYPTIAGDIYAAATIVYEMLTATTPYSGHVSTILARQLCEP
                     VEPPSQRAPGRGISPAIDRVVLRALDRSPDARYPSVTAFAEALDEALDGRPEPAIAAG
                     SGLWRNPTLRGLPPTRAPAPSAGSQDPADAIISKALAT"
     gene            complement(1168..1833)
                     /locus_tag="VF516_40575"
     CDS             complement(1168..1833)
                     /locus_tag="VF516_40575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114116.1"
                     /translation="MASTAIGLLLGLLVGLRHSFEPDHLTAVSTLVGETRDLRGGAML
                     GALWGVGHTLALVAVGAILMGVGASLPERAAVIFELCVAAMLLVLGARAIVVALGAAA
                     AVQHAHIGPHLGLHGRPHPVVRTLVWRPLTVGLVHGLAGSGALTAIAFAELPGAGARL
                     LYMTLFGLGSIAGMALASGAAGVTLRVIVRSGGARRGLALVTGALSITVGVMWSIPLW
                     SRL"
     gene            complement(1852..>3789)
                     /locus_tag="VF516_40580"
     CDS             complement(1852..>3789)
                     /locus_tag="VF516_40580"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEX8114117.1"
                     /translation="SVRPGDDFFAYANGGWLKAHEIPPDRATYGTGAIVQELTSKRTA
                     ELIAEAARDAPAGSDARKVGDTYASYLDEAAIAARGAAPLAPVLQQIAAIADRRALAT
                     ALGAALRADVDVLNNTNLDTPNLFGLWVAQDLSDPAHYAAFLLQGGLGLPDRDDYRDP
                     SPRMAEVRDRYQAHVAAMLALAGLSDAAARAARVVALEHEIAAVHALRVDTENVERGN
                     NHWTRGELADRAPGLDWTAFLAAAGLDRQPQFVVWQPGALVGLAALVASQPLDAWKDW
                     LAFHAIERRADVLPKAFDDELFAFHGKVLSGTTQQRERWKRAVDATSAALGEAVGKLY
                     VTRYFPASEKARAAQMVSNEIAAFARRIDHLAWMAPATKAKAKAKLAALRVGVGYPDT
                     WRDYSRLEVVRGDACGNAERAERFEYRRDLDKLGRAVDRGEWVMTPQLVNAVNLPAMN
                     ALNFPAAILQPPFFDPDRPIVMDYGAVGAVIGHEISHSFDDQGALFDATGRLDNWWTP
                     EDFAHFKASSAALARQYDGYRPFPDLAVNGALTNSENIADVAGLAAAYDAYRIAFGGQ
                     EAPSVAGLTGDQLFFLSFAQSWRRKSREPALRRQILTNGHAPAEYRGDTVRNIDAWYM
                     AFHVEPGQALYLAPDQRVRVW"
BASE COUNT          520 a         1340 c         1439 g          490 t
ORIGIN      
        1 gtggccagcg ccttcgagat gatcgcgtcg gccggatcct gggatccggc ggacggcgcc
       61 ggcgcgcggg ttggtggcaa accgcgcagc gtcgggttgc gccagaggcc ggagccggcc
      121 gcgatcgccg gctcggggcg cccgtcgagc gcctcgtcga gcgcctcggc gaacgcggtc
      181 acgctcgggt agcgcgcgtc cggcgaccgg tcgagcgcgc gcagcaccac gcgatcgatc
      241 gccggcgaga tgccgcggcc gggggcgcgc tgcgacggcg gctcgaccgg ctcgcacagc
      301 tggcgggcca ggatggtcga gacgtgcccg ctgtacggag tcgtggcggt cagcatctcg
      361 tagacgatcg tcgcggcggc gtagatgtcg ccggcgatcg ttggatactc gccggcgatc
      421 atctcgggtg ccatgtacgc cggggtgccg gcgacatcgt cggtggtgcc cggcgacgcc
      481 gacaccgggc aggcgagccc gaagtcgatg atggtgacga tgtcgacctt gtcgaccagc
      541 acgttgccgc tcgtgaggtc ggcgtggacg atcccggcgg cgtggatcgc ggccagcccg
      601 gcgaacagct gcgacgcgat cgcggccacc cgctcgcgcg gctgcgggcc gcaggcggcg
      661 atcatgtcgc acagcggcgt gccgtgcgcg cggtccatga ccaggaacgg ccggccgtcg
      721 tggccggtcc cgtggtcgag cacccgcacc acgttggggt gcgagaccgc gcggagcagc
      781 cgcgcctcgt cggccagccg cgccgccagc agctggttgc tggacagcgt gttgcgcacg
      841 cgcttgatca cgacggcccg gcccgagcgg tggcgagcgg catgaacctg ccccatcccg
      901 cctttcccga ggagctcacc gagctggtac tggccgacca tggctcgcga caaagcactc
      961 ggcgtgccga cgccgtgccg gcggttcgcc cgcggcgggc cctgccgttc cgcggatctc
     1021 gaatacggga cagcacgtac gaggccgggt ccgcgcatcg gctacccagg ggcccccgag
     1081 tcggggaaca cccggtgcag caccgtgcac gcgcggggtg aagcgaccgg atcgaccggg
     1141 ctggtcaggc aggtccggcg tcgccattca cagccgcgac cacagcggga tcgaccacat
     1201 cacaccgacg gtgatcgaca gggcgccggt gaccagagcc aggccgcggc gcgcgccgcc
     1261 cgaccggacg atgacgcgca gcgtgacccc ggcggcgccc gatgcgagcg ccatcccggc
     1321 gatcgacccc aggccgaaaa gcgtcatgta caggagccgc gccccggcac ccgggagctc
     1381 ggcgaacgcg atcgcggtga gcgcgccgct gccggcgagg ccgtgcacca ggcccacagt
     1441 gagcggccgc cacacgagcg ttcgcacgac agggtggggg cggccgtgca gcccgagatg
     1501 tggtcctatg tgcgcgtgct ggacggccgc cgcggcgccg agcgcgacga cgatcgcccg
     1561 ggcgccgagc accagcagca tcgccgcgac gcacagctcg aagatcaccg cggcgcgctc
     1621 cggcaggctc gcgccgaccc ccatgaggat cgcgccgacc gcgaccagcg ccagcgtgtg
     1681 gccgacgccc cacagcgcgc cgagcattgc accgccccgc aggtcgcggg tctcgccgac
     1741 cagcgtcgat accgcggtca ggtgatcggg ctcgaagctg tggcgcagcc cgacgagcaa
     1801 cccgagcagg agtccgatcg ccgtcgaggc cacgccgcag catacatgca atcaccagac
     1861 ccgcacgcgc tggtcgggcg ccaggtacag cgcctgaccg ggctcgacgt gaaacgccat
     1921 gtaccaggcg tcgatgttgc ggaccgtgtc gccgcggtac tcggccgggg catggccgtt
     1981 ggtcaagatc tggcggcgga gcgcaggttc gcgcgacttg cggcgccagc tttgcgcgaa
     2041 gctgagaaag aacagctggt cgccggtcag cccggccacg gagggggctt cctggccgcc
     2101 gaacgcgatc cggtaggcgt cgtaggccgc ggcgaggccg gcgacgtcgg cgatgttctc
     2161 gctgttggtg agcgcgccgt tgaccgcgag atcggggaac ggccggtagc cgtcgtactg
     2221 ccgggccagc gccgcgctcg acgccttgaa gtgagcgaag tcctccggcg tccaccagtt
     2281 gtccagccgg ccggtcgcgt cgaacagcgc gccctgatcg tcgaagctgt ggctgatctc
     2341 gtggccgatc accgcgccca ccgcgccgta gtccatgacg atcggccggt cgggatcgaa
     2401 gaacggcggc tgcaggatcg ccgccgggaa gttgagcgcg ttcatcgccg gcaggttgac
     2461 ggcattgacc agctgcgggg tcatcaccca ctcgccgcga tcgacggcgc ggccgagctt
     2521 gtcgaggtcg cggcggtact cgaaccgctc ggcgcgctcg gcgttgccgc aggcgtcgcc
     2581 gcgcacgacc tcgagccggc tgtagtcgcg ccaggtgtcg gggtagccga cgccgacccg
     2641 gagcgcggcg agcttggcct tggccttggc cttggtcgcg ggcgccatcc acgccaggtg
     2701 gtcgatccgg cgcgcgaacg ccgcgatctc gttgctcacc atctgcgcgg cgcgcgcctt
     2761 ctccgacgcc gggaagtagc gggtgacgta cagcttgccg accgcctcgc cgagcgcggc
     2821 gctggtcgcg tcgaccgcgc gcttccagcg ctcgcgctgc tgggtcgtgc cgctgagcac
     2881 cttgccgtgg aacgcgaaca gctcgtcgtc gaacgccttc gggagcacgt cggcgcggcg
     2941 ctcgatcgcg tggaacgcga gccagtcctt ccaggcatcg agcggctggc tggcgaccag
     3001 cgcggcgagg ccgaccagcg cgccgggctg ccagaccacg aactgcggct ggcggtcgag
     3061 cccggcggcg gccaggaacg ccgtccagtc gagcccgggc gcgcggtcgg cgagctcgcc
     3121 gcgggtccag tggttgttgc ccctctcgac gttctcggtg tcgacccgca gcgcgtggac
     3181 ggcggcgatc tcgtgctcga gcgcgacgac ccgcgcggcg cgggcggcgg cgtcggacag
     3241 cccggcgagc gcgagcatcg cggcgacgtg cgcctggtac cggtcgcgga cctcggccat
     3301 ccgcggcgac gggtcgcggt agtcgtcgcg atcgggcagg ccgagcccgc cctggagcag
     3361 gaacgccgcg tagtgcgccg ggtcgctgag gtcctgcgcg acccacaggc cgaacaggtt
     3421 cggggtgtcg aggttggtgt tgttgagcac gtcgacgtcg gcgcgcagcg cggcgccgag
     3481 cgcggtggcg agcgcgcggc gatccgcgat cgcggcgatc tgctggagca ccggagccag
     3541 cggcgcggcc ccgcgggcgg cgatcgccgc ctcgtcgagg tagctcgcgt aggtgtcgcc
     3601 gaccttgcgc gcatccgaac cggcgggcgc gtcgcgcgcg gcctcggcga tcagctccgc
     3661 ggtgcgcttg gaggtgagct cctggacgat cgcgccggtg ccgtacgtcg cgcgatccgg
     3721 cgggatctcg tgggccttga gccagccgcc gttggcgtag gcgaagaagt cgtcgcccgg
     3781 ccgcaccga
//
LOCUS       DASVOR010002190         3603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_402573,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002190 DASVOR010000000
VERSION     DASVOR010002190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402573"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..685
                     /gene="ccsA"
                     /locus_tag="VF516_40585"
     CDS             <1..685
                     /gene="ccsA"
                     /locus_tag="VF516_40585"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEX8114118.1"
                     /translation="ALVGAAFVAHGVDIGWRGVEHVHPAQSVREALGFLAFIITGGYL
                     LASARYRLTLGGAAVMPVSLILLVAARLTPAGAAPEDLSALGRIHISLATIGVGVFAL
                     ASALSVIYLAEERALKRKKFDAIAFKDRGAPLEALDRLSHRLIWIGFPIFTVALVLGA
                     VWVSRRGESLDRPQYPLAAVTWISYAALLVLRQVYGWRGRRAARLTLAGFAAALLVLV
                     LYLVRRMTG"
     gene            688..2073
                     /gene="hemA"
                     /locus_tag="VF516_40590"
     CDS             688..2073
                     /gene="hemA"
                     /locus_tag="VF516_40590"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEX8114119.1"
                     /translation="MSELFVVGISWRTAPVAVREKLAFRDDELPGALRAMTAELPVAE
                     ALLVSTCNRVEVYGVGKPGADPTGPVRAFLAAQRGLAPADVAEVLYDHRGNAAVRHVF
                     SVASALDSLVLGEAQILGQLKAAYGVAGAAGTSGPLLGRCLERAFGVAKRVRTETAIA
                     RGAANVSTVAVDLAKRVFGNLAGKSVLVVGAGKMSTLAARHLYASGAQHIVVTNRSPE
                     KAEALAAEIDGIARPWADLEGHLVEADVVISSTGARAPILTRPLFKRVTKARRWRQLV
                     VIDIAVPRDAEPQIADFDGVYVFDIDDLEKVVAANLAERARAAEHAARIVEHEAGQFQ
                     HWMRTQGVVPTIRALRERFARVADVELQKTLDQLARRERTPAQQREAIQRLVQLVVNK
                     LLHPPTIALREAPPDEAGLRAEILCQLFELSPVAAPDPTASAGPAAQPEPAPTLAELA
                     EPAPSERKAVT"
     gene            2070..2996
                     /gene="hemC"
                     /locus_tag="VF516_40595"
     CDS             2070..2996
                     /gene="hemC"
                     /locus_tag="VF516_40595"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEX8114120.1"
                     /translation="MTQRLVIATRGSALALWQAEHVKARLAAVAPDVAVEFNVIKTSG
                     DQILDVPLAKVGGKGLFVKEIEQALLDHQADLAVHSMKDVPAELAPGLVLVAVSSREV
                     PWDALCARAPVTVDTLPRGARVGTSSMRRQCQLLARRPDLQIAMLRGNVPTRIRKLDD
                     GEFDAVVLAAAGLTRLGLAGRITQQLPLEVSVPAVAQGVLGLEARAGDARVAELIRRA
                     IHDPAEERRVTAERSFLARMGGSCQTPLAAHAIDAGAGQLRIVGLCGMPDGSRILRAE
                     HTGAAADAEPLGRRLADDLLAQGADQILAATR"
     gene            3021..3416
                     /locus_tag="VF516_40600"
     CDS             3021..3416
                     /locus_tag="VF516_40600"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HEX8114121.1"
                     /translation="MIRYAAAAPQFTKIAVTVPTETYAVIERLRRRLGKSRSAVVALA
                     LEEWVRSVDLAEADRRYVEGYLRTPEAVDEIGAVAAQATSHWASWSPGQPLRAEDSSP
                     GQPAKRTAGAPRAGRAGRAKRAAPKRSRR"
     gene            3413..>3603
                     /locus_tag="VF516_40605"
     CDS             3413..>3603
                     /locus_tag="VF516_40605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114122.1"
                     /translation="MRRGEIWWARLALPAGRRPVALVSRDAAYLIRSSVTVAEVSTVV
                     RAIPSEIALGTRDGMPRPCV"
BASE COUNT          437 a         1351 c         1337 g          478 t
ORIGIN      
        1 cgcactggtc ggtgccgcgt tcgtggcgca cggcgtcgac atcggctggc gcggcgtcga
       61 gcacgtccac cccgcacagt cggtccgcga ggcgctcggg ttcctggcgt tcatcatcac
      121 gggcggttac ctgctggcca gcgcgcgcta ccggctgacc ctgggcggcg cggcggtgat
      181 gccggtgtcg ctgatcctgc tggtcgcggc gcgcctgacg ccggccgggg ccgcgcccga
      241 ggacctctcg gcgctcggcc ggatccacat ctcgctggcg accatcgggg tcggcgtgtt
      301 cgcgctggcc agcgcgctgt cggtgatcta cctcgccgag gagcgcgcgc tcaagcgcaa
      361 gaagttcgac gcgatcgcgt tcaaggaccg cggcgcgccg ctcgaggcgc tcgaccggct
      421 gagccaccgc ctgatctgga tcgggttccc gatcttcacg gtcgcgctcg tgctcggcgc
      481 ggtctgggtg tcgcggcgcg gcgagtcgct cgaccgcccg cagtatccgc tggccgcggt
      541 gacctggatc tcgtacgcgg cgctgctcgt gctccgccag gtctacggct ggcgcggccg
      601 gcgcgcggcg cgcctgaccc tggccgggtt cgccgcggcg ctgctcgtgc tcgtgctcta
      661 cctcgtgcgg cggatgacgg gctagggatg agcgagctgt tcgtcgtcgg catcagctgg
      721 cggaccgcgc cggtggcggt ccgcgagaag ctggcgtttc gcgacgacga gctgcccggc
      781 gcgctgcgcg cgatgaccgc cgagctgccg gtcgccgagg ccctcctggt gtcgacctgc
      841 aaccgggtcg aggtctacgg cgtgggcaag cccggcgccg acccgaccgg tccggtgcgg
      901 gccttcctcg ccgcccagcg cgggctcgcg ccggccgacg tcgccgaggt gctctacgac
      961 caccgcggca acgcggcggt gcgccacgtg ttctcggtcg ccagcgcgct cgactcgctg
     1021 gtgctcggcg aggcccagat cctcggccag ctcaaggcgg cgtacggggt cgccggcgcc
     1081 gccgggacct cggggccgct gctcggccgc tgcctcgagc gcgcgttcgg ggtcgccaag
     1141 cgggtgcgga ccgagaccgc gatcgcgcgc ggcgcggcca acgtgtcgac cgtcgcggtc
     1201 gatctcgcca agcgcgtgtt cggcaacctc gccggcaaga gcgtgctggt ggtcggcgcc
     1261 ggcaagatgt cgaccctggc ggcgcgccac ctctacgcgt cgggcgccca gcacatcgtc
     1321 gtcaccaacc gctcgcccga gaaggccgag gcgctcgccg ccgagatcga cggcatcgcc
     1381 aggccgtggg ccgacctcga gggccacctc gtcgaggccg acgtcgtgat cagctcgacc
     1441 ggcgcgcgcg cgccgatcct gacccggccg ctgttcaagc gggtgaccaa ggcgcggcgc
     1501 tggcgccagc tcgtggtgat cgacatcgcg gtgccgcgcg acgccgagcc gcagatcgcc
     1561 gacttcgacg gggtctacgt gttcgacatc gacgatctcg agaaggtcgt cgccgccaac
     1621 ctcgccgagc gggcgcgcgc cgccgagcac gcggcccgga tcgtcgagca cgaggccgga
     1681 cagttccagc actggatgcg cacccagggc gtcgtgccga cgatccgcgc gctgcgcgag
     1741 cggttcgccc gggtcgccga cgtcgagctc cagaagacgc tcgaccagct cgcccggcgc
     1801 gagcgcaccc cggcgcagca gcgcgaggcg atccagcgcc tggtccagct cgtggtcaac
     1861 aagctgctgc acccgccgac gatcgcgctg cgcgaggcgc cgcccgacga ggccggcctg
     1921 cgcgccgaga tcctgtgcca gctgttcgag ctgtcgccgg tcgcggcgcc cgatccgacc
     1981 gcgtcggcgg ggcccgccgc gcagcccgag cctgcgccca cgctcgccga gctcgccgag
     2041 ccggctccct ccgaacgaaa ggcagtgacg tgacccagcg cctggtgatc gcaacccgcg
     2101 gcagcgccct cgccctgtgg caggccgagc acgtcaaggc caggctcgcc gccgtcgccc
     2161 ccgacgtggc agtcgagttc aacgtgatca agacgtcggg ggatcagatc ctcgacgtgc
     2221 cgctcgccaa ggtcggcggc aaggggctgt tcgtcaagga gatcgagcag gcgctgctcg
     2281 atcaccaggc cgacctcgcg gtccacagca tgaaggatgt ccccgccgag ctcgcgcccg
     2341 gcctcgtgct ggttgcggtg tcgtcgcgcg aggtcccctg ggacgcgctg tgcgcgcggg
     2401 cgccggtcac ggtcgacacc ctgccgcgcg gcgcccgggt cggaacgtcg tcgatgcgcc
     2461 gccagtgcca gctgctcgcg cgccggcccg acctccagat cgcgatgctg cgcggcaatg
     2521 tcccgacccg gatccgcaag ctcgacgacg gcgagttcga cgcggtggtg ctcgccgcgg
     2581 ccggcctgac ccggctggga ctcgccggcc ggatcaccca gcagctgccg ctcgaggtct
     2641 cggtccccgc agtcgcccag ggcgtgctcg gcctcgaggc ccgcgccggc gacgcccggg
     2701 tcgccgagct gatccggcgc gcgatccacg accccgccga ggagcgccgg gtcaccgccg
     2761 agcgcagctt cctggcgcgg atgggcggca gctgccagac gccgctcgcg gcccacgcga
     2821 tcgacgccgg cgccggccag ctgcgcatcg tcggcctgtg cggcatgccc gacggcagcc
     2881 ggatcctgcg cgccgagcac accggcgccg cggccgacgc cgagccgctc ggccgccggc
     2941 tcgccgacga tctgctcgcc cagggcgccg accagatcct cgcggcgacc cggtagcgcg
     3001 gctcatggta tgatgccggt atgattcggt atgccgcggc tgcgccgcag ttcaccaaga
     3061 tcgcggtcac ggtgccgacc gagacctacg ccgtgatcga gcggctgcgg cgccggctcg
     3121 gcaagtcgcg cagcgccgtg gtcgcgctcg cgctggagga gtgggtccgc agcgtcgacc
     3181 tcgccgaggc cgaccggcgg tacgtcgagg gctatctccg gaccccggag gcggtcgacg
     3241 agatcggcgc ggtcgcggcc caggccacgt cgcactgggc cagctggtcc ccgggacagc
     3301 cgttgcgcgc cgaggactcg tcaccgggtc agccggcgaa gcgaaccgcc ggtgcgccac
     3361 gagcgggccg agccggccgg gcgaagcgag ccgcaccgaa gcggtcgcgg cgatgaggcg
     3421 cggcgagatc tggtgggcgc ggctggcgtt gccggccggt cgccggcccg tggcgctggt
     3481 gtcgcgcgac gccgcctacc tgatccgctc gtcggtcacg gtcgcggagg tctcgaccgt
     3541 ggtccgcgcg atcccgagcg agatcgcgct cggcacccgc gacgggatgc cgaggccgtg
     3601 cgt
//
LOCUS       DASVOR010002191         4500 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_112134,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002191 DASVOR010000000
VERSION     DASVOR010002191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112134"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VF516_40610"
     CDS             complement(<1..259)
                     /locus_tag="VF516_40610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114123.1"
                     /translation="MRSLAIGLLLLPGLVSAAEAAPKLTLDQVIARALANPRAQAAES
                     DTAGAEARVEEADAARLPRGKVTAFGTISPEIHCVTVNLVEC"
     gene            complement(334..1509)
                     /locus_tag="VF516_40615"
     CDS             complement(334..1509)
                     /locus_tag="VF516_40615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8114124.1"
                     /translation="MLGAIVRILLSSSLLAGIHVYLWLRLVRPAQLSPRWRLAATLGL
                     VALFLSIPITVASRLAFPALSATLGWISLPWMALGGLTFVTLIAIDTVRLAGRLAQAR
                     RSRAPAGPVTALSRRQFLARVSGGTALAIGSGSMVRGMAEARGDHEVVDVEIALARLP
                     RALDGFTIVQLSDLHAGMTIDRGFVERVVDRANRLAPDLIALTGDLVDGRVEELRPSI
                     EPLGRLRARHGVFAVTGNHEYYAGADAWIAELTRLGARYLRNQRVAIGDGAASFDLAG
                     VDDYSAAGWPGHGEDLPAALAGRDRARALVLLAHQPRQVHVAAQHGVDLQLSGHTHGG
                     QIWPWHYIVRMQQGGLLAGRYQIAGTQLYVSRGCGYWGPPVRLLAPLEITRVILRTA"
     gene            1596..2039
                     /locus_tag="VF516_40620"
     CDS             1596..2039
                     /locus_tag="VF516_40620"
                     /inference="COORDINATES: protein motif:HMM:NF016056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HEX8114125.1"
                     /translation="MAVADGTRAGSAPESPDGPIVLYDGDCGFCARSVRWILAHERDP
                     AIRFAPLQGTTAARLRRSYPRIPEQIASVVYVADGRAHLRSKAMLHIARHLRAPWRWV
                     HALCWLPGFVFDVGYRVLAAVRYRIWGHADACQLVTPEQRARFLP"
     gene            2157..3230
                     /locus_tag="VF516_40625"
     CDS             2157..3230
                     /locus_tag="VF516_40625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114126.1"
                     /translation="MTRCLCALAIFVAGCPMPGTAPQAPGPPAPVASGDAGCPAADHI
                     LVASYLASDDPAQPAPHTGWVLPLYDQKVDTLVGQPEYTALDAATASARGVPPAPPQV
                     WLTVPGQPPCRAVIGSYYAAAVEGAIPNVTYGVELDGCAAPPRDQQDDAEALALVSEQ
                     APTGCQLLAPQPVAARIGETDAQKHWQRPTKQTPLPPAIAAIVPPHACSAPGCEMLWA
                     FGQVEVAGRPVAWAGAVNWLTIPPGASPDSQCSWKADTFAGFFVAAADGSAVKLTEGQ
                     DHPLLLTAVLADRGGAKALVAEGPGEYTTYDLTAGARVARHLVWLRLPPEAYAIDERI
                     GPSCGGDAAGSPPAGSPPAGPRR"
     gene            complement(3237..>4500)
                     /locus_tag="VF516_40630"
     CDS             complement(3237..>4500)
                     /locus_tag="VF516_40630"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEX8114127.1"
                     /translation="ARSAGSEGADRPKPPAPAAAASLVVAAPPAKTIAPPVRGVTDDS
                     VTLGMSAAFSGASRELGNRMKLGLDTAFAAVNEAGGVAGRSIKLLALDDGYEGKRALA
                     NVQELITDRNVFAIIGDVGTPTTVEALPYVLSKKALFFGAFTGSKILRRDPPDRYVFN
                     YRASYEDETAKMVNYLIDVKKIPDRGIVVFAQHDSYGDAGYDGATKMLRKKGHNDEIL
                     RVSYERNTLDVDDAVSKVVEYNNATVRVGPNDYARKHPVKAILMVGTYKASAKFIQKL
                     RERKIDAILINVSFVGSTALAEELKELGVNPGGVIVTQVVPHFDSGATGIIRYREALK
                     KFHPDQQPDFISLEGYIVGQLFAEGLRRVGRELDTEKLIDALEHIQDYDPGTGSALGF
                     SPSQHQASHKVWGTMLDDQGVFKSIDME"
BASE COUNT          571 a         1618 c         1679 g          632 t
ORIGIN      
        1 cgcactcgac caggttgacg gtcacgcagt ggatctcggg gctgatcgtc ccgaacgccg
       61 tcaccttgcc ccgcggcagc cgggccgcgt cggcctcctc gacccgggcc tcggctcccg
      121 cggtgtcgct ctcggcggcc tgcgcgcggg ggttcgccag cgcccgcgcg atgacctggt
      181 cgagcgtcag cttgggagcg gcctcggccg cggagacgag gcccggcaac agcagcagtc
      241 cgatggcgag cgagcgcatg gcccgcgttg tactacgccg cgtggcgccg ggccggtgtg
      301 cgcggccgct cgcgtgggcc gcgtcgctcc ggttcaggcg gtgcgcagga tcacccgcgt
      361 gatctcgagc ggcgcgagca gccggaccgg cggaccccag tagccgcagc cgcgcgacac
      421 gtagagctgc gtgcccgcga tctggtagcg gccggcgagc aggccgccct gctgcatccg
      481 cacgatgtag tgccacggcc agatctggcc gccgtgggtg tggcccgaca gctggaggtc
      541 gacgccgtgc tgcgcggcga cgtggacctg gcggggctgg tgggcgagca gcacgagcgc
      601 gcgcgcccga tcgcggccgg cgagcgcggc gggcagatcc tcgccgtggc cgggccagcc
      661 cgccgcggag tagtcgtcga ccccggcgag gtcgaagctc gcggcgccgt cgccgatcgc
      721 gacccgctgg ttgcgcaggt agcgcgcgcc gagccgggtc agctcggcga tccacgcgtc
      781 ggcgccggcg tagtactcgt ggttgccggt caccgcgaac acaccgtggc gcgcccgcag
      841 ccggccgagc ggctcgatcg acgggcgcag ctcctcgacc cggccgtcga ccaggtcgcc
      901 ggtcagcgcg atcagatcgg gcgcgagccg gttggcgcgg tcgacgaccc gctcgacgaa
      961 cccgcggtcg atcgtcatgc cggcgtgcag atccgagagc tggacgatgg tgaacccgtc
     1021 gagcgcgcgc ggcaacctgg cgagcgcgat ctcgacgtcg acgacctcgt ggtcgccgcg
     1081 cgcctcggcc atgccgcgga ccatgctgcc gctgccgatc gcgagcgcgg tgccgccgct
     1141 gacccgggcg aggaactgcc ggcgcgacag cgccgtgacc ggaccggccg gcgcccggga
     1201 ccgccgggcc tgggccagcc ggcccgccag ccgcaccgta tcgatcgcga tcagcgtgac
     1261 gaaggtcagc ccgccgagcg ccatccacgg cagcgagatc cagcccaggg tcgccgacag
     1321 cgcggggaat gcgagccggc tcgccaccgt gatcgggatc gacaggaaca gcgcgactag
     1381 gccgagcgtc gccgcgagcc gccagcgcgg ggacagctgc gccgggcgga ccagccgcag
     1441 ccacaggtag acgtggatcc cggcgagcag cgagctgctg agcaggatgc ggacgatcgc
     1501 cccgagcacg cccgaccata gcgcgccggg accggccatg cagcccgggc acgcccggcg
     1561 gttgccgccg ccccgggacg gggcaagctg tccccatggc ggtcgccgat ggtacgcggg
     1621 ccgggtcggc gccggaatcc cccgacgggc cgatcgtgct gtacgacggc gactgcgggt
     1681 tctgcgcccg gtcggtgcgc tggatcctcg cacacgagcg cgaccctgcg atccggttcg
     1741 ccccgctgca gggcacgacc gccgcgcggc tgcgccggag ctaccctcgg attcccgagc
     1801 agatcgccag cgtggtctac gtcgcggacg ggcgcgccca cctgcgcagc aaggcgatgc
     1861 ttcacatcgc ccggcacctc cgcgcgccgt ggcgctgggt gcatgcgctg tgctggctgc
     1921 ccggcttcgt gttcgacgtc ggctaccgcg tactcgcggc cgtccgctac cggatctggg
     1981 gccacgccga cgcgtgccag ctcgtcacgc ccgagcagcg ggcgcggttc ctgccgtgat
     2041 ccgcggcgtc agcagcgctg gacggttccc ccatgcagcg ccaactatcg ggacctgcat
     2101 cacgcaggcg ccgcggcgtg gctgtcggcc gcgtgcgatt gtggcaaacc tcgtgcatga
     2161 cgcgatgcct ctgtgcgctg gcgatcttcg tggcgggctg tccgatgccg gggacggccc
     2221 cgcaggcacc ggggccgccg gcgccggtcg cgtccggcga cgcgggctgt ccggccgccg
     2281 atcacatcct ggtcgcgtcg tacctcgcca gcgacgatcc ggcgcagccg gcgccgcata
     2341 ccggctgggt gctgccgctg tacgatcaga aggtcgacac gctggtcggc cagcccgagt
     2401 acacggcgct cgatgcggcg accgccagcg cgcgcggcgt gccgcccgcg ccaccgcagg
     2461 tctggttgac ggtgccgggc cagccgccgt gtcgcgcggt gatcggctcg tactacgcgg
     2521 ccgccgtcga gggggcgatc ccgaatgtca cctacggcgt cgagctcgac ggctgcgcgg
     2581 cgccgccccg cgatcagcag gacgacgccg aggcgctcgc gctggtgtcc gagcaggcgc
     2641 cgaccgggtg ccagctgctg gcgccgcagc cggtggccgc gcggatcggg gagaccgatg
     2701 cccagaagca ctggcagcgg ccgaccaagc agacgccgct gccgccggcg atcgcggcga
     2761 tcgtcccgcc ccacgcgtgc agcgcgccgg gctgcgagat gctgtgggcg ttcggccagg
     2821 tcgaggtcgc cgggcggccg gtggcgtggg ccggcgcggt caactggctg accatcccac
     2881 cgggcgcctc gccggactcg cagtgcagct ggaaggccga cacgttcgcc gggttcttcg
     2941 tcgcggccgc cgacggcagc gcggtcaagc tcaccgaggg ccaggaccat ccgctgctgc
     3001 tcaccgcggt gctcgccgac cgcggcggcg cgaaggcgct ggtcgccgag ggccccggcg
     3061 agtacacgac ctacgatctc accgccggtg cgcgggtggc gcgccacctg gtctggctgc
     3121 ggctgccgcc cgaggcctat gcgatcgacg agcggatcgg gccgagctgc ggcggggacg
     3181 cggccgggtc gccgcccgcc ggctcgccac ccgctggccc gcggcgctga ccgccgtcac
     3241 tccatgtcga tcgacttgaa cacgccctga tcgtccagca tcgtgcccca gaccttgtgg
     3301 ctggcctggt gctgggacgg gctgaagccg agcgcgctgc cggtgcccgg gtcgtagtcc
     3361 tggatgtgct ccagcgcgtc gatcagcttc tcggtgtcga gctcgcggcc gacgcggcgc
     3421 aggccctcgg cgaacagctg gccgacgatg tagccctcga gcgagatgaa gtcgggctgc
     3481 tgatcggggt ggaacttctt gagcgcctcg cggtagcgga tgatgccggt ggcgcccgag
     3541 tcgaagtgcg ggacgacctg ggtcacgatc acgccgccgg ggttgacgcc gagctccttg
     3601 agctcctcgg ccagcgcggt gctgccgacg aacgacacgt tgatcaggat cgcgtcgatc
     3661 ttgcgctcgc ggagcttctg gatgaacttc gcgctcgcct tgtacgtccc gaccatgagg
     3721 atcgccttga ccgggtgctt cctggcgtag tcgttgggcc cgacccggac cgtggcgttg
     3781 ttgtactcga cgaccttgct gaccgcatcg tcgacgtcga gcgtgttgcg ctcgtagctg
     3841 acccgcagga tctcgtcgtt gtggcccttc ttgcgcagca tcttggtcgc gccgtcgtag
     3901 cccgcatcgc cgtagctgtc gtgctgcgcg aacacgacga tcccgcggtc cgggatcttc
     3961 ttgacgtcga tcaggtagtt gaccatcttg gcggtctcgt cctcgtagct cgcccggtag
     4021 ttgaagacgt agcggtccgg tggatcgcgg cgcaggatct tcgacccggt gaacgcgccg
     4081 aagaacagcg ccttcttgga cagcacgtag ggcagggcct cgaccgtggt cggcgtgccg
     4141 acgtcgccga tgatcgcgaa cacgttgcga tcggtgatca gctcctggac gttggccagc
     4201 gcgcgcttgc cctcgtagcc gtcgtcgagg gcgagcagct tgatcgagcg tccggcgacg
     4261 ccgccggcct cgttgaccgc cgcgaacgcg gtgtcgaggc cgagcttcat gcggttgccg
     4321 agctcgcgcg acgcgccgga gaacgcggcg ctcatgccca gcgtgaccga gtcgtcggtc
     4381 acgccgcgca ccggcggggc gatcgtcttg gcgggtggcg cggcgacgac gaggctggcc
     4441 gcggcggcgg gggccggggg cttcggccgg tccgcgcctt cgcttcccgc cgagcgggcg
//
LOCUS       DASVOR010002192         3519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_24826,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002192 DASVOR010000000
VERSION     DASVOR010002192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24826"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VF516_40635"
     CDS             complement(<1..250)
                     /locus_tag="VF516_40635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8114128.1"
                     /translation="MVAEATHTPASPKSIAIAVPDGVKLPIVMDNHSTTAVALRVVQA
                     MLPYFTQHYGNAASRNHSFGWTAEAAVEHARAQVAALLG"
     gene            complement(250..864)
                     /locus_tag="VF516_40640"
     CDS             complement(250..864)
                     /locus_tag="VF516_40640"
                     /inference="COORDINATES: protein motif:HMM:NF020839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEX8114129.1"
                     /translation="MTKDDSNKLMRVGELAKAVGKTVRAMHLYEELGLLEPRARSEGG
                     FRLYGPEAIDRIHWIVKLQAIGFTLAEIQGFVGDFQHADSGREATSRVRALFTEKQAQ
                     IREQIMQLQVIENDLSEALSYLDSCQTCSDDYAPTACGVCDHHGHQKGEAPRLFAGLS
                     RLPGQAAVAPGTPGPAGPASNDDTRDKPRDFDVPVTVLYREGNN"
     gene            complement(1043..1180)
                     /locus_tag="VF516_40645"
     CDS             complement(1043..1180)
                     /locus_tag="VF516_40645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114130.1"
                     /translation="MRDDEEDPKTPHPVSTVKKRSNPAETAEAVPIRKDDERDHHRHV
                     V"
     gene            complement(1296..2267)
                     /locus_tag="VF516_40650"
     CDS             complement(1296..2267)
                     /locus_tag="VF516_40650"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEX8114131.1"
                     /translation="MANPNLPGERSATPPEETEPSWRPQDPDLPDERGRPGDDDDESG
                     GGGELRDARRWDSDEPYRSAERAGDRPGAGRYGEERLRELQRGPRRDERSQWGGGDPD
                     WRPSAGSFEDRYRELGADAGIGSWPDHTGSDPWRYASQSQSAYRGRDFEPERHGLSGG
                     HDPRTGHAGGRPRRGDERLGEPAGSYSRPGAWDAADATSSASREVHVHRGTGPHRGKG
                     PAGYQRSDERIRELVCESLTDDDEIDASHIEVGVSHGEVTLSGTVEDRRIKRAAEDCA
                     YSVSGVRDVQNRLRVHDDRASRSNPSGAAAVDPHETQSSAQGSKPRG"
     gene            complement(2427..>3519)
                     /locus_tag="VF516_40655"
     CDS             complement(2427..>3519)
                     /locus_tag="VF516_40655"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEX8114132.1"
                     /translation="KAWIDEARARAAAAVRAVDTTAADPAHEREARRVMEIAAARYQH
                     HYLTERSASNLRIGAELVAALLDNGGALHAALERVAGVQLQVNDDRDAVRLDSLDGVG
                     KEMVRRAIGKLIKKPETLDDARRDPEAWAAKARDHLQQEIRALGKRAFQTLGLPRAHP
                     EIVELVGALNFRTSYTQNQWWHAVEASYLAGMMAAELGLDEKLARRATLMHDIGKALT
                     HKIEGSHAVIGADIARRLGEPEVVANAIGAHHADEPCNSVYAHLVAASDAMSGARPGA
                     RRELAEGFTAKIEDLERIGLSRRGVAYAHAVHGGRELRVYVREREVDDLTVVEMSTDI
                     ANQIAEEMTFPGQIKVTVIRAFEATATAN"
BASE COUNT          412 a         1267 c         1255 g          585 t
ORIGIN      
        1 cgccgagcag cgccgcgacc tgcgcccgcg cgtgctcgac cgcggcctcc gcggtccagc
       61 cgaacgagtg gttgcggctg gcggcgttgc cgtagtgctg cgtgaagtac ggcagcatcg
      121 cctggacgac ccgcagagcg accgcggtcg tcgagtggtt gtccatgacg atcggcagct
      181 tcacgccgtc ggggaccgcg atcgcgatcg acttcggcga tgccggcgta tgggttgcct
      241 ctgcgaccat tagttattgc cctctcgata aagcacggtg accgggacgt cgaagtcccg
      301 tggtttgtcg cgggtgtcgt cgttgctggc cgggcctgcc gggccgggcg tcccaggcgc
      361 gaccgcggcc tgccccggca ggcgggacag cccggcgaac agccgcggcg cctcgccctt
      421 ctgatgaccg tggtgatcgc acacgccgca cgcggtcggc gcgtagtcgt cgctgcaggt
      481 ctggcacgag tcgaggtacg agagcgcctc gctcaggtcg ttctcgatca cctgcagctg
      541 catgatctgc tcgcggatct gggcctgctt ctcggtgaac agcgcacgga cccgcgaggt
      601 cgcctcgcgc cccgagtcgg cgtgctggaa atcgccgacg aagccctgga tctcggcgag
      661 cgtgaacccg atcgcctgca gcttgacgat ccagtggatg cggtcgatcg cctcgggccc
      721 gtacaggcgg aagccgccct ccgaccgggc gcgcggctcc agcaagccca gctcctcgta
      781 gaggtgcatc gcgcggacgg tcttgccgac cgccttggcg agctcgccga cccgcatcaa
      841 cttgttggaa tcgtccttgg tcatcgcggc cttcaaaccc tgacgcatac gtaaggttga
      901 gtgcgtctga ccggactatg ctggcttacg atcgacctgt caaggtgaac cctcaccttt
      961 acgtgggggt catgagggaa cttcgcagag ggagcgagtc gcgtcgcgcg gctcgtcgat
     1021 cgaccgggga cgcgtggcgc cgtcagacca cgtggcggtg gtggtcgcgc tcgtcgtcct
     1081 tgcggatcgg gaccgcctcg gcggtctcgg cggggttgga gcgcttcttg acggtcgaca
     1141 cggggtgcgg cgtcttcgga tcctcttcgt cgtcgcgcat ggcgacatgg ctcggtgcag
     1201 atccggtgcc cgaaaaagcg atcaggcaag gcgccgtcac tcgccttgcc tgaccacacc
     1261 ccgggcgacg ttgcccgagg tgcacagacg ttgattcacc cgcgcggctt gctgccttgc
     1321 gcggagctct gggtctcgtg cggatcgacc gcggcggcgc ccgaggggtt gctccgcgac
     1381 gcgcggtcgt cgtgcacgcg cagccggttc tggacgtcgc gcacgccgga cacgctgtac
     1441 gcgcagtcct ccgccgcgcg cttgatgcgg cgatcctcga ccgtcccgga cagcgtcacc
     1501 tcgccgtggc tcacgccgac ctcgatgtgc gaggcgtcga tctcgtcgtc gtcggtcagc
     1561 gactcgcaga ccagctcgcg gatccgctcg tcggagcgct gatagcccgc ggggcccttg
     1621 ccgcgatgcg ggccggtgcc gcggtgcacg tggacctcgc gggacgcgct gctcgtcgcg
     1681 tccgcggcgt cccacgcgcc ggggcggctg tacgagcccg ccggctcgcc gagccgctcg
     1741 tctccgcgcc ggggacggcc gccggcgtgg cccgtgcgcg ggtcgtggcc cccggacagc
     1801 ccgtgtcgct cgggctcgaa gtcgcgaccg cggtacgcgc tctggctctg gctggcgtag
     1861 cgccacggat cgctgccggt gtgatctggc caggacccga tgccggcgtc ggctccgagc
     1921 tcgcgatagc ggtcctcgaa gctgccggcc gatggccgcc agtcagggtc cccacccccc
     1981 cactggctac gctcgtcgcg ccgcgggcca cgctggagct cgcgcagccg ttcttcgccg
     2041 tagcggccgg cgccggggcg atcgccggct cgctcggcgg accggtacgg ctcgtcggag
     2101 tcccaccgcc gcgcgtcgcg cagttcaccg ccgccgcccg actcgtcatc gtcgtcgccc
     2161 gggcggccgc gctcgtcggg caggtccgga tcttgcgggc gccagctcgg ctcggtttcc
     2221 tcggggggcg tcgcgctgcg ctctcccggc aagttggggt tcgccacggg catgctcctg
     2281 tgcagttgtc gatcgcttcg ttgtgcatcg cgcgtgcccc gccgccccgg cagcgtcgtg
     2341 cgccgggtgc gcgcgtcgtg ggcgcacggc gtggcagcgg cgcactcggc ggttcgcgga
     2401 aatccgtggc gggtgcgcgc cggggctcag ttcgccgtcg cggtcgcctc gaacgcgcgg
     2461 atcaccgtga ccttgatctg accggggaac gtcatctcct cggcgatctg gttcgcgata
     2521 tcggtcgaca tctcgaccac cgtcagatca tcgacctcgc gctcgcggac gtagacccgg
     2581 agctcgcggc cgccgtggac cgcgtgcgcg tacgccacgc cgcgccggga cagcccgatc
     2641 cgctcgagat cctcgatctt cgcggtgaag ccctcggcga gctcgcggcg cgctcccggg
     2701 cgggcgccgc tcatcgcgtc ggacgccgcg accaggtggg cgtacaccga gttgcagggc
     2761 tcgtcggcgt ggtgcgcacc gatcgcgttg gccaccacct cgggctcgcc gagccggcgc
     2821 gcgatgtcgg cgccgatcac cgcgtgcgag ccctcgatct tgtgggtcag cgccttgccg
     2881 atgtcgtgca tcagcgtcgc gcgccgtgcg agcttctcgt cgaggccgag ctcggccgcc
     2941 atcatcccgg cgaggtacga cgcctcgacc gcgtgccacc actggttctg cgtgtagctg
     3001 gtgcggaagt tgagcgcgcc gaccagctcg acgatctcgg ggtgcgccct gggcagcccc
     3061 agggtctgga acgcgcgctt gccgagcgcg cggatctcct gctgcaggtg atcgcgcgcc
     3121 ttcgccgccc acgcctcggg atcgcggcga gcgtcgtcga gcgtctccgg cttcttgatc
     3181 agcttgccga tcgcgcgccg gaccatctcc ttgccgacgc catcgaggct gtcgagccgg
     3241 accgcgtcgc ggtcgtcgtt gacctggagc tggaccccgg cgacccgctc gagcgcggcg
     3301 tgcagcgcgc cgccgttgtc gagcagcgcc gcgaccagct cggcgccgat ccgcaggttc
     3361 gacgccgagc gctcggtcag ataatggtgc tggtagcgcg ccgcggcgat ctccatcacg
     3421 cggcgcgcct cgcgttcatg cgccggatcc gcggcggtgg tgtcgaccgc gcggaccgcg
     3481 gccgccgccc gggcgcgggc ctcatcgatc cacgccttc
//
LOCUS       DASVOR010002193         5706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_53319,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002193 DASVOR010000000
VERSION     DASVOR010002193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5706
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53319"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            151..1392
                     /locus_tag="VF516_40660"
     CDS             151..1392
                     /locus_tag="VF516_40660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114133.1"
                     /translation="MRDIEDLGAEIEHAWRDAQYRAEEFAAIAQAALERRALPAHTWV
                     ERIFRWFLSAPQIPHQPPHFAFGEPPIQLYAGRRFYIEALFWTDGTTAIHEHCFSGAF
                     QVLMGGSIHTTYRFERTETISRELVLGKLAADRSELLRAGDIRPIVSGDRFIHSLFHL
                     ERPSLTLVVRTRHDAGSGPQYVYRPPGIAYDPFIPDERAARLLRLLEVLDPQRPETSG
                     LLAEMAASADLGSVVAMLVHWCSLHPIDPGACDALLAIVGRRHRALAESLRAAIQEAR
                     RQALIIHRRRSHRRPEHRFFLAMLLHVGDREQILQLVTQQFPGADPIELIMTWVEELV
                     TAPSGSVPGTMQNAGLEYDLGEAELKILHHLLRRRTPEQVVMALNEEYDEVEDRRPEI
                     LDLCASLTRSALFQPLFRMSP"
     gene            1389..2537
                     /locus_tag="VF516_40665"
     CDS             1389..2537
                     /locus_tag="VF516_40665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114134.1"
                     /translation="MSELMLNPNLRVQPVLTDGRVTAYSLLALVRGRGRLASTVAIAA
                     ASPEVTRVLKGLSDADADVEVDETAFASLVDLGVLVQDHEISRGVGFSCRLPVGAPVA
                     PAALTVNPQLAITAFEDFARTTGLVGVLEPCTWIAVVADPVTGARWPYWIDDRDHALL
                     LRLVPGAPPPAELDAAELDRLAGAGILVDADAVAYARQRIDAAAAQFARSRYAELPGL
                     LPAPQMLALCSYYRGLRNEGHLFSQDDLVPLRHAMPSERVMQYYHRELYGLFARVIGD
                     PIKPSYAYLASYRRGAALARHLDREQCKFTASLLLEYLAGPSDDRVWPIHLELPASDE
                     PVAVHLEPGDCLLFSGCDQPHYRQELRGERSTSFLLHYVDEAFSGSLE"
     gene            complement(2565..3569)
                     /locus_tag="VF516_40670"
     CDS             complement(2565..3569)
                     /locus_tag="VF516_40670"
                     /inference="COORDINATES: protein motif:HMM:NF025043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HEX8114135.1"
                     /translation="MSQESPLDIPYRLHPRLVSSIKSSGSMVVSGAEQAVEVPLDMFQ
                     ALMMFSEPRTARQAFDMLDVDVDLDEFGRIVDGFVEHGLLRRDQASDDGPSLRELLDP
                     AVFGDAARVDEIAGWMRAGRAIIIPDALHGDLAEAVHDDLQRLTEWTPSESGHDFFHH
                     RSCVVDRIAGRSPALTRCGQLFTSAATRRFMAELSGQDCAGESHAAASWYRPGEYALP
                     HDDFATGYARSVAFIWYLTKDWRQEWGGALFWCPTGQYIRPGFNVLIMFRVMPANVHL
                     VCPVALGATARRLTINGFWHRSVRSSPPDRIPEAALVSPRAYGPPPPEASGSSPVLVL
                     "
     gene            complement(3672..4178)
                     /locus_tag="VF516_40675"
     CDS             complement(3672..4178)
                     /locus_tag="VF516_40675"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8114136.1"
                     /translation="MKDTIDVLELLESQHGEVDQLIEDLERGRGDRAALFAELADKVA
                     AHSAVEEKIFYPAAMAPNTEELLHEFVEDHLTVKRLLADMMEMDPDDEDGEFDETLAL
                     LKDEFDRHSHDQEEGRLFPRLRRMMSADERAGLGNEVLAMFEWLIDQEPRRDVPRETA
                     EPAPLPPP"
     gene            4202..5047
                     /locus_tag="VF516_40680"
     CDS             4202..5047
                     /locus_tag="VF516_40680"
                     /inference="COORDINATES: protein motif:HMM:NF014017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEX8114137.1"
                     /translation="MPALNTAAGRGHQLVMARIYAGTSGWVYRSWRDHIYAGAPVRRW
                     LEIASREFSSLEINGSFYTQISQATYARWRAETPAGFRFALKGHRFVTHYKRLRDCAP
                     SIARLRDQASQLGDKLAAVVWQLPSNFALDLGRLDDFAAALRGWPVRHAIELRHRSWF
                     LPEVAARLRAAGIAVCMGDAPDFPMWREITADFVYVRLHGHTRKYASSYSAAALRRWA
                     ADALRWRDRGLDVFVYFDNDAEGHAVRNAQTFARLIDRREEPPVGSTSGRARRVPGAP
                     ARRWP"
     gene            complement(5031..5537)
                     /locus_tag="VF516_40685"
     CDS             complement(5031..5537)
                     /locus_tag="VF516_40685"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8114138.1"
                     /translation="MPNRMDSMLSKGAGMMKGVKARLEGLTGVFKTLAEQHGEVAAML
                     KRLQSKPEKKPELWPEIRRELLSHERGEVREVYPVLRQHVQTRALAEHHDQEAAEMER
                     LIERIDTAMADDWRPLFDQLVDAVIHHANQEESDIFPKAQEAIGDKATKDLEHRFLAA
                     KKQVMATA"
BASE COUNT          859 a         1974 c         1928 g          945 t
ORIGIN      
        1 agagcagtga cccgaaggaa tcgagcaaga agaagctcgt ccttcgcaag gagtccatcc
       61 gggccctcga cggagtgaac ctggcactgg tggccggcgg cgccgatacc gcggccacct
      121 gcaatccccc gcccaatcgc ccgacctgac gtgcgcgaca tcgaagacct cggcgcggag
      181 atcgagcacg cctggcgcga cgcccagtac cgcgcggagg agttcgcggc gatcgcgcag
      241 gcagcgctcg agaggcgagc gctgcctgca cacacctggg tcgagcggat ctttcgctgg
      301 ttcctgtcag cgccgcagat cccccatcag ccgccgcact tcgcgttcgg cgagccgccg
      361 atccagctct acgccggtcg ccggttctac atcgaggcgc tgttctggac cgacggaacc
      421 accgcgatcc acgagcactg cttctcgggc gcgtttcagg tgctgatggg cggcagtatt
      481 cacacgacat atcggtttga acgcacggag acgatcagtc gcgagctggt cctgggcaag
      541 ctcgcggccg accgatcgga gctcctgcgc gccggcgaca tccgaccgat cgtctccggc
      601 gatcgcttca tccactcgct gttccatctc gagcggccgt cgctgacgct ggtcgtgcga
      661 acccggcacg acgccggcag cggtccgcag tacgtgtatc gtcctcccgg tatcgcctat
      721 gacccgttca tccccgacga gcgcgcggcg cgcctgctcc ggctcctcga ggtcctcgac
      781 ccccagcggc cggagacctc ggggctgctc gccgagatgg cggcgagcgc cgacctcggt
      841 tcggtcgtcg cgatgctcgt gcactggtgt tcgctccacc ccatcgatcc cggggcatgc
      901 gatgcgctgc tcgcgatcgt cgggcgccgc catcgcgcgc tggcggagag cttgcgcgcg
      961 gcgatccagg aggcgcgccg gcaggcgctg atcatccacc gcagacggag ccatcgccgc
     1021 ccggagcatc gcttcttcct ggcgatgctg ctccatgtcg gagatcgcga gcagatcctg
     1081 cagctcgtga cgcagcagtt tcccggcgcc gatccgatcg agctgatcat gacgtgggtc
     1141 gaggagctgg tcaccgcacc ctcgggatct gtccccggca cgatgcagaa cgccggcctc
     1201 gaatacgacc tgggcgaggc cgagctcaag atcctgcacc acctgctgcg ccggcgaacg
     1261 cccgagcagg tcgtcatggc gctgaacgag gaatacgacg aggtggagga ccggcggccg
     1321 gagatcctgg acctgtgcgc gtcgctgacc cgatcggcgt tgttccagcc gttgttccgg
     1381 atgtcgccat gagcgagctg atgttgaatc cgaacctgcg ggtccagccc gtgctcaccg
     1441 acggccgcgt gaccgcgtac tccctgctgg cgctggtcag aggccgaggc cgcctggcca
     1501 gcaccgtcgc gatcgcggcg gcgagcccgg aggtcacgcg cgtgctgaag ggcctgtccg
     1561 acgccgacgc cgacgtcgag gtggacgaga ccgccttcgc atcgctggtc gacctcggcg
     1621 tgctggtcca ggaccacgag atctcgcgag gggtcgggtt ctcgtgtcgc ctcccggtcg
     1681 gggcaccggt cgctcccgcg gcgctgacgg tgaatcccca gctcgcgatc accgcgttcg
     1741 aggacttcgc gcggaccacc gggctggtgg gcgttctcga gccatgcacg tggatcgccg
     1801 tggtggcgga cccggtgacc ggcgcgcgct ggccgtactg gatcgacgac cgcgatcatg
     1861 cgctgttgct gcggctggta ccgggtgctc ccccacccgc cgagctcgac gccgccgagc
     1921 tcgaccggct ggccggggcc ggcatcctcg tcgatgcaga cgccgtcgcg tacgcccgcc
     1981 agcgcatcga cgccgcggcg gcgcagttcg cgagatcccg gtacgcagag ctgccggggc
     2041 tgctgccggc accgcagatg ctggcactgt gcagctacta tcgaggcctc cggaacgagg
     2101 gccacctgtt ctcgcaggat gaccttgtcc cgctacggca cgcgatgccc agcgaacgag
     2161 tgatgcagta ctatcaccgc gagctgtacg gcctgttcgc gcgcgtgatc ggtgacccga
     2221 tcaagccgtc ctatgcctac cttgcgtcgt atcgccgcgg cgcggcgctg gcaaggcacc
     2281 tcgatcgcga gcagtgcaag ttcacggcgt ccctgctcct cgaatatctc gccgggccgt
     2341 ccgatgaccg ggtctggccg atccatctcg agctgccggc gagcgatgaa ccggtcgccg
     2401 tgcacctcga gccgggtgat tgcttgctgt tcagcggatg cgatcagccg cactatcgac
     2461 aggagctccg cggcgagcgc tccacgtcgt tcctgctgca ctatgtcgac gaggccttct
     2521 cgggcagtct ggagtagacg ctccctgacc tggcagccgg ggcgctacag cacgaggacc
     2581 ggtgacgagc ccgacgcctc gggcggcggg gggccgtacg cgcgcggcga gacgagcgcg
     2641 gcctcgggaa tccggtccgg tggcgagctt cgcaccgagc ggtgccagaa cccgttgatc
     2701 gtcagtcgcc tggcggtcgc accgagagca accgggcaga ccaggtgcac gttcgcgggc
     2761 atcacgcgga acatgatcag cacgttgaat ccgggcctga tgtactgtcc ggtcggacac
     2821 cagaacagcg cgccgcccca ctcctgtcgc cagtccttcg tgaggtacca gatgaacgcg
     2881 acggagcgag cataaccggt ggcgaagtca tcgtgtggca gagcatattc cccgggccgg
     2941 taccagctgg ccgccgcgtg cgactcgccg gcgcaatcct gtcccgagag ctcggccatg
     3001 aagcgccggg tcgccgcgct ggtgaacagc tgaccgcatc tcgtcaatgc aggacttcgg
     3061 cctgcgatac ggtcgacgac gcagcttcga tggtgaaaga aatcatggcc actttccgaa
     3121 ggagtccatt ccgtcaatcg ctgcaagtcg tcatgcacgg cctcggcgag atctccatgc
     3181 agtgcatccg gaatgatgat cgcccggccg gctcgcatcc agccggcgat ctcgtccacg
     3241 cgtgcggcgt caccgaacac cgccgggtcg agcagctccc gcagggaggg gccgtcatcg
     3301 ctggcctggt ctcgtcggag caggccgtgc tccacgaagc catcgacgat cctgccgaat
     3361 tcatccagat cgacatcgac gtcgagcatg tcgaacgcct gccgcgccgt cctcggctcc
     3421 gagaacatca tgagtgcctg gaacatgtcg agcggaacct cgaccgcctg ctcggcacca
     3481 gagactacca tggatccgga actctttatc gagctcacga ggcgcggatg gagtcgatag
     3541 ggtatatcga gcggcgattc ctggctcatg gtacgaaccg ggaagcgatg tagcgcggac
     3601 gaacgtcggc ggtcaagcac gggcggactt tgccaggtcg ccgatgccgg acgcgacgta
     3661 cgcgatgtgc ttcacggcgg gggcagcggg gcgggctcgg cggtctcgcg cggcacgtcg
     3721 cgccgcggct cctggtcgat cagccactcg aacatcgcga gcacctcgtt gcccaggccc
     3781 gcgcgctcgt ccgcggacat catccgccgc agccggggga acagccggcc ctcctcctgg
     3841 tcgtgcgagt ggcggtcgaa ctcgtccttg agcagcgcca gggtctcgtc gaactcgccg
     3901 tcctcgtcgt cggggtccat ctccatcatg tccgcgagca ggcgcttgac ggtgaggtgg
     3961 tcctcgacga actcgtgcag cagctcctcc gtgttcggag ccatcgccgc ggggtagaag
     4021 atcttctcct cgaccgccga gtgcgcggcc accttgtcgg cgagctcggc gaacagcgcg
     4081 gcgcggtcgc cgcgaccacg ctcgagatcc tcgatcagct gatcgacctc gccgtgctgc
     4141 gattcgagga gctcgagtac gtcgatggtg tccttcatgt tgcagatgcg ttgcagtcga
     4201 ggtgccagcc ctgaacacgg cggccgggcg cggtcaccag ctggtgatgg cgcggatcta
     4261 cgcaggcacc agcggctggg tctaccggtc gtggcgggac cacatctacg ccggcgcgcc
     4321 ggtccggcgc tggctggaga tcgcgtcgcg cgagttctcg tcgctcgaga tcaacggatc
     4381 gttctatacg cagatctcgc aggcgaccta cgcgcggtgg cgagcggaga cgcccgcggg
     4441 gttccggttc gcgctcaagg gccaccgctt cgtcacgcat tacaagcggc tgcgcgactg
     4501 cgcgccgtcg atcgcgcggc tgcgcgatca ggccagccag ctcggggaca agctcgcggc
     4561 ggtggtctgg cagctgccga gcaacttcgc gctcgacctg ggtcggctcg acgatttcgc
     4621 ggcggcgctg cgcggctggc cggtccgcca cgcgatcgag ctccgccacc gctcgtggtt
     4681 cctccccgag gtcgcggcgc ggctgcgcgc ggccgggatc gcggtgtgca tgggcgacgc
     4741 cccggacttt ccgatgtggc gcgagatcac ggcggacttc gtctacgtcc ggctgcacgg
     4801 ccacacccgc aagtacgcgt cgagctacag cgccgcggcg ctgcgccggt gggcggcgga
     4861 cgcgctgcgc tggcgcgatc gcgggctcga cgtgttcgtg tacttcgaca acgacgccga
     4921 gggccacgcg gtgcgcaacg cgcagacgtt cgcccgcttg atcgaccgta gggaggagcc
     4981 ccccgtggga tcaacgagcg gccgcgcgcg gcgcgtgcca ggcgcgccgg ctaggcggtg
     5041 gccatgacct gcttcttcgc ggcgaggaac cggtgctcga ggtccttggt cgccttgtcg
     5101 ccgatcgcct cctgcgcctt ggggaagatg tcgctctcct cctggttggc gtggtggatc
     5161 acggcgtcga cgagctggtc gaacagcggg cgccagtcgt cggccatcgc ggtgtcgatc
     5221 cgctcgatca ggcgctccat ctcggcggcc tcctgatcgt ggtgctcggc gagcgcgcgg
     5281 gtctgcacgt gctggcgcag cacgggatag acctcgcgaa cctcgccgcg ctcgtgcgac
     5341 agcagctcgc gccggatctc cggccacagc tcgggcttct tctcgggctt gctctgcagc
     5401 cgcttgagca tcgccgccac ctctccgtgt tgctcggcga gcgtcttgaa gacgccggtc
     5461 agcccttcga ggcgcgcctt gactcccttc atcatccctg cgcccttcga cagcatcgaa
     5521 tccatacgat ttggcatggg ggtctccttc acgccaatcg agtgcatcgg acatgccgcc
     5581 gcgccgtgcg cgcacgcgcg gtcggcgtcg aacccgagca ttcccgatca ccgtgcgccg
     5641 ggatcgcgag cccgcgtgga atacgcgttg cttgatcgcg actgtccgac aacggccctt
     5701 ccacag
//
LOCUS       DASVOR010002194         1967 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_402614,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002194 DASVOR010000000
VERSION     DASVOR010002194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402614"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="VF516_40690"
     CDS             complement(<1..827)
                     /locus_tag="VF516_40690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEX8114139.1"
                     /translation="MSDTGRNKTVVTAISKISDRPVAKEACLVVIYGLELGKKFNLNR
                     PQIVIGRSSKADIQIDQEAVSRNHCKIMNSGSNIMLRDMGSTNGTYVNDEMIDEYVLR
                     DGDFIKVGRCIFKFLSGNNIENAYHEEIYRLTTVDGLTQVFNRRYFVETLEREIGRAL
                     RYRRDLSLIMFDIDRFKGVNDSFGHLAGDHVLKHLASVIKTRIRREDVLARYGGEEFA
                     IVLPEIDGYNAMQFAEKIRKLVEVAEFKFEDAIIPVTVSIGVASLRGEIEDALEFIK"
     gene            complement(892..1266)
                     /locus_tag="VF516_40695"
     CDS             complement(892..1266)
                     /locus_tag="VF516_40695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114140.1"
                     /translation="MLGPHPRLDRADRDLNGDAVAEAIVVDRSLCSEEGNCYWNVFVV
                     PPPGSSECARYVGTFEGGALEPLTTTGDDNMADVRGYWNLHGGRLLLQSYRFLHGVYR
                     LVDALLCKRAHDDTLVCADSER"
     gene            1522..>1967
                     /locus_tag="VF516_40700"
     CDS             1522..>1967
                     /locus_tag="VF516_40700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114141.1"
                     /translation="MRRSHYLLAGAVVVAGAAALVGNRTTTPALVRGEPFDPVRPAPG
                     VVVEPGSAAGPGAVAVDRLPGRAPAVSTTSIADLIDLDRRELAAGGAHYEAPLKDGRR
                     AVLTLDPTLQAMAEKLLSEARAPRGAIVAMAPDGRILALAGRRSDPV"
BASE COUNT          296 a          684 c          654 g          333 t
ORIGIN      
        1 tgcttgatga actcgagcgc gtcctcgatc tcgccgcgca ggctggcgac cccgatcgac
       61 accgtcaccg ggatgatcgc gtcctcgaac ttgaactcgg cgacctcgac cagcttgcgg
      121 atcttctcgg cgaactgcat cgcgttgtag ccgtcgatct cgggcagcac gatcgcgaac
      181 tcctcaccgc cgtagcgcgc gagcacgtcc tcgcgccgga tccgggtctt gatcaccgac
      241 gccaggtgct tgagcacgtg gtcgcccgcg aggtgtccga agctgtcgtt gacgcccttg
      301 aagcggtcga tgtcgaacat gatcagcgac aggtcgcggc ggtagcgcag cgcgcggccg
      361 atctcgcgct ccagggtctc gacgaagtag cgccggttga acacctgggt cagcccgtcg
      421 acggtggtca gccggtagat ctcctcgtgg tacgcgttct cgatgttgtt gccggacagg
      481 aacttgaaga tgcagcggcc gaccttgatg aagtcgccgt cgcgcagcac gtactcgtcg
      541 atcatctcgt cgttgacgta ggtgccgttg gtcgacccca tgtcgcggag catgatgttg
      601 ctgccgctgt tcatgatctt gcagtggttg cgggacaccg cctcctggtc gatctggatg
      661 tcggccttcg acgaccggcc gatcacgatc tggggccggt tcaggttgaa cttcttgccg
      721 agctccaggc cgtaaatgac gaccaggcac gcctccttgg ccaccggacg gtcgctgatc
      781 ttcgagatcg cggtgacgac ggtcttgttc cggccggtgt cgctcaaccc gccttcgatt
      841 ttatcagaat tcgtcacggg acgtcccttc gatctccctt cgttcgggga tctagcgctc
      901 cgaatccgcg cacaccagtg tgtcgtcatg ggcgcgcttg cacagcagcg cgtcgacgag
      961 gcggtaaaca ccgtgcagaa acctgtacga ctgcaacagc aaccgtcctc cgtgtaggtt
     1021 ccagtagccg cgaacgtcgg ccatgttgtc gtcccccgtc gtggtcagcg gttccagcgc
     1081 gccgccctcg aaggtcccga catagcgcgc gcactccgac gagccgggcg gcggcaccac
     1141 gaacacgttc cagtagcagt tgccctcctc gctgcacagc gagcgatcga cgacgatggc
     1201 ctcggcgacg gcgtcgccgt tgaggtcccg gtcggcccgg tcgaggcggg ggtgcggtcc
     1261 cagcacccca tcgcgacccg ggctggcaca cgagccgacc tggaccgacg cggccgggct
     1321 cgggcgcggc gcggccggcg gggctgcccg gtgacacgcg gtcagcgcga ccgcggcccc
     1381 gcagaggctg gctcggagac ctgcccggag acctgagaag ccgatccacg acctgcgcat
     1441 gcgcaattgc ggcggggaac ctacatcgaa acggcgaaac attcttgtgc ggacgggcag
     1501 atgacgttac atccaaggcc gatgcgacgc tcccactacc tgctggccgg ggccgtggtg
     1561 gtcgccggcg ccgcagcgct cgtgggcaac cgcacaacca cccccgccct ggtgcgcggc
     1621 gagccgtttg acccggtgcg tccggctccg ggagtcgtgg tcgaaccggg ttccgcggcc
     1681 ggtcccgggg ccgtcgccgt cgatcgtctc cccggccgcg cgccggcggt ctcgacgacc
     1741 tccatcgccg atctgatcga cctcgatcgc cgagagctcg ccgccggcgg cgcgcactac
     1801 gaggccccgc tcaaggacgg gcgccgcgcc gtgctgaccc tcgacccgac gctgcaggcg
     1861 atggccgaga agctgctgtc cgaggcgcgc gcgccgcgcg gggcgatcgt cgcgatggcg
     1921 cccgacggcc ggatcctcgc gctcgccggc cggcgcagcg atcccgt
//
LOCUS       DASVOR010002195         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_461740,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002195 DASVOR010000000
VERSION     DASVOR010002195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461740"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="VF516_40705"
     CDS             complement(<1..181)
                     /locus_tag="VF516_40705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114142.1"
                     /translation="MADGPMADLAGRKLGDFVLREQIGEGGCAVVYRCEQPQLRRSAV
                     VKVLHERGQRSDAALD"
     gene            1735..>2145
                     /locus_tag="VF516_40710"
     CDS             1735..>2145
                     /locus_tag="VF516_40710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114143.1"
                     /translation="MARTAKTVEQRVAELRSLDAAAPGSLDQLRTALRGTSGFLVAAA
                     AQRVQDDRLEPLVPELAPAFERLREDAVKRDPGCRGKLAIARALHALDHWEDRVFVAG
                     LAHEQREGWGQEDTAAELRGVCGLAHAHFARADAL"
BASE COUNT          322 a          746 c          741 g          336 t
ORIGIN      
        1 gatcgagcgc ggcgtcgctg cgctgccccc gctcatgcaa taccttgacg accgcgctgc
       61 gccggagctg tggctgctcg cagcggtaca ccaccgcaca gccgccctcg ccgatctgct
      121 cgcggaggac gaaatccccc agcttgcgcc ctgcgagatc tgccatcggc ccgtctgcca
      181 tcggtgtgct gattagaaca ccaggcgacc cgctggcgcg cggtctgggg cgtcgcattt
      241 tgcggcgatt ttcctactgt gagtagatgg tatgcctaca taggccaccc cccggtaggt
      301 atatgcggag gagcttccga cccgattcga agaacggacc ctcgtcgctt cccgcggcgg
      361 gggcgttctt gcttgccggc ccatccgtcg cctgcgcctt ggcctcccca gagggcgacg
      421 tactcggccc cgtcaaggac gctcgctgcg ctcgcgccgc acggcggcgg gcttcgcccg
      481 tcctggacgg agcctccgtg cgccgccatg gccaggtgcc gggtcgccac aggcgacccc
      541 ggtcgtcgag cttcccatcg gaaatcaggc cgaggatctg acctgacgat ctgtgacgtg
      601 actccgtagc acgtacggag tgggggagtt agatttggaa tgtacaacgg gcccactcgc
      661 cgtcgcaagc cgctacggga cagcaagctg ccgccgtcgc ctgggccgcc gctgcgtcgc
      721 ccgggcctcc gctgcgagga gcggccgaag caggtgggcg cccacgcccc ggcacgcccc
      781 ggcataggcg ccctcgccac agccagccgt gagtaaccgt gaggccgacg agcgcacggc
      841 gcgctatcgg aaatattggg atgcgaccgg cagcgttcac ggtggtcact ccggcgagga
      901 tgagcccgtg ccaaggcgct gccggtcgcc cccttgaggc accgaatgcg aacgctgttg
      961 ctccttgccg ctgcatgcgg ccatgtcacc cctggcgcgt cccaggttcc accggacgct
     1021 actccagaac ctccaagctt ctgccctatc aacgtcgtgg atcacgcgct accgtcgtct
     1081 tgggtcatgc aaggtccctt cgacctggtc gccgatccaa ccgggagagg cgcacaggtc
     1141 tgggtctcca acgccaatgt gccttcacag gcgtcgttcg ccttgccgtt caaggtcggc
     1201 gatcggatga ccgggcctgg ctgacgagct cagcgtcgac gatgccgccg gcatcgatcg
     1261 agccgacgtg gccgcggagc aacttctccg gatctggcga gaggaagctc gacgagccgg
     1321 accgggagcg atcgaacgtt ttgcgcggcg cacattggtc ggggagttgc gtcgagatct
     1381 gccggaaggt tggcgagccg actggacacg caaggaccca gagactacgg cggcggtatc
     1441 gcgggcggcg caggcgctgg ggcggtgggt ctgctacctg agcgacgacg acaagcccgc
     1501 ggccgatcgc gttcttgatg caatggtcgc ggctgggcgg gacggcacca tcccgccggg
     1561 gtggttgcct tcaggaccag atgatcccgt tctggtcgac atgttccggc gacattggcc
     1621 ggaagccaag taactcgagc gcgcgggggg atttcgtcga acgcgcttcg agcgcttcgg
     1681 gctcgcacgc accggctggt ccggccggag gatctcggga atactgcgcc cacgatggcc
     1741 aggacggcca agacggtcga gcaacgggtc gccgagctac gcagcctcga cgccgccgcg
     1801 ccgggctcgc tcgaccagct gcgcaccgcg ctgcgcggga ccagcggctt cctggtcgcc
     1861 gccgcggccc agcgggtcca ggatgaccgg ctggagccgc tggtgcccga gctcgccccg
     1921 gcgttcgagc ggctgcgcga ggatgcggtg aagcgcgatc ccggctgccg cgggaagctc
     1981 gcgatcgcgc gcgcgctcca tgcgctcgac cactgggagg accgcgtgtt cgtcgccggc
     2041 ctggcccacg agcagcgcga gggctggggc caggaagaca ccgccgccga gctgcgcggc
     2101 gtgtgcggcc tcgcgcatgc ccacttcgcc cgcgccgacg cgctc
//
LOCUS       DASVOR010002196         4064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_374320,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002196 DASVOR010000000
VERSION     DASVOR010002196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4064
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374320"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VF516_40715"
     CDS             <1..892
                     /locus_tag="VF516_40715"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8114144.1"
                     /translation="MITRGSQMALDLVARAIVRPGDVVCVEALGYRPSWAALERAGDR
                     LIGVPVDAGGIDVDALARICARAAVRLVYVTPHHQYPTTAVLTAGRRARLLALARQHR
                     IAVLEDDYDHEFHYEGRPVLPLATTDRHGAVVYIGTLSKILAPGLRLGFVVAPRPLID
                     RLARERYLVDRQGDHALEIAIAELIEDDELGRHTRKMRRIYQARRDALAAALRDQLGD
                     RVRFHVPTGGIALWVETAPGLDADRWCKRGLAHGVVFQPGSAFSLAGKRVPAARLGYG
                     ACTEAEIRLAVRRLAQCARP"
     gene            958..1260
                     /locus_tag="VF516_40720"
     CDS             958..1260
                     /locus_tag="VF516_40720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4258 domain-containing protein"
                     /protein_id="HEX8114145.1"
                     /translation="MNAIDPAVEPVGRTLRTVVIALLEAGRVSLPVHASERMDERHLT
                     APQIETALRSGALASESCDGGAWRYRASKQGVVVIFTFDTDDDGNLLVVVTTWRTR"
     gene            1257..1814
                     /locus_tag="VF516_40725"
     CDS             1257..1814
                     /locus_tag="VF516_40725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HEX8114146.1"
                     /translation="MKCTECGNKMTRSVVDHVYRESGLDNVILHGITRYRCESCGAER
                     VQIPNMGPLHRAIARAIAHKPARLVPAEVRFLRDHLELSNKDFAALMGVTPEQASRWT
                     SSEPIGVPAERFLRTLALLGPGVVSLDQGDDREDDSLPDPELSPSLFKALLRELPPPS
                     TAPREVPIDLRRAPSGWKLEAHAAS"
     gene            complement(1839..2477)
                     /locus_tag="VF516_40730"
     CDS             complement(1839..2477)
                     /locus_tag="VF516_40730"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEX8114147.1"
                     /translation="MVKFWLMKSEPDQFGIADLERVGVEPWTGVRSYFARAHMRAMAV
                     GDAVLFHHSNARPPGVAGLARVMRTGVVDETQFDPDSKYFDPKATRDAPIWDCVAVEY
                     VATFPHFVTMDRIRDEPALADMLLLRRGMRLSVQPVTEAEYERIVALGHSAPVERPAP
                     ARPARPGKPRKPEQPAKAAKAPKAKSSKPARGKPARSLKAARRGARARGSRR"
     gene            2554..3165
                     /locus_tag="VF516_40735"
     CDS             2554..3165
                     /locus_tag="VF516_40735"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8114148.1"
                     /translation="MVSLASIPAGEYVPTADERIVMYNVPWSHFEVQLALRGDASSPR
                     MAYLDGALELMSPSKGHERTKSYLGCLIEVFAMERDLVLSPYGSWTLKGPPELAGVEP
                     GECYIVGPDQDKERPDLAIEVFWTSGSINKLEIYRRLKVGEVWSWKDGVLSVHVLSAG
                     RYQQVERSALFPELDLALLCTFLDRRSATEAMRDFRNVLRARP"
     gene            complement(3176..>4064)
                     /locus_tag="VF516_40740"
     CDS             complement(3176..>4064)
                     /locus_tag="VF516_40740"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8114149.1"
                     /translation="ATARRRHASDQTCARQLAWSGSLPPGLELTWLGTAGFRLAYQGT
                     VAWIDPYVTRLSLRDLVARHVVPPSEAAIAAWVDRADAVLVGHTHFDHALDVPAIARR
                     FGARVYGSSSLAQLMKIHGLAGQAVVVAPHRDYEVGPFRFRFAPSRHSKLQLGLAVPY
                     AGELTCEHVDELTPQRYRCGQVWGICIEVAGMRIYHQGSADLIEDEIRERGVDLFLCG
                     IAGRRFTPRYVERIVRALAPDRIVPTHYDDFFRPLDAPLRFSLNVDLTGFAEEVHRVS
                     RDVPVATLEIGAPIGAASN"
BASE COUNT          590 a         1534 c         1365 g          575 t
ORIGIN      
        1 catgatcacg cgcggcagcc agatggcgct cgacctggtc gcccgcgcga tcgtccggcc
       61 gggcgacgtg gtctgcgtcg aggcgctcgg ctatcgcccg tcgtgggccg cgctcgagcg
      121 cgccggcgac cgcctgatcg gcgtcccggt cgacgccggc ggcatcgacg tcgatgcgct
      181 cgcccggatc tgcgcccgcg ccgccgtccg cctggtctac gtcaccccgc accaccagta
      241 cccgaccacc gccgtgctca ccgccggccg ccgcgcccgg ctgctcgcgc tcgcccgcca
      301 gcaccggatc gccgtcctcg aggacgacta cgaccacgag ttccactacg agggccggcc
      361 ggtcctgccg ctcgccacca ccgaccgcca tggcgccgtc gtgtacatcg gcacgctgtc
      421 caagatcctc gcgcccggcc tgcgcctggg cttcgtcgtc gccccgcgcc cgctgatcga
      481 ccggctcgcc cgcgagcgct acctcgtcga tcgccagggc gaccacgccc tcgagatcgc
      541 gatcgccgag ctgatcgagg acgacgagct cggccgccac acccgcaaga tgcggcggat
      601 ctaccaggcg cggcgcgacg ccctcgccgc cgcgctgcgc gaccagctcg gcgaccgcgt
      661 ccgcttccac gtcccgaccg gcggcatcgc cctgtgggtc gagaccgccc cgggcctcga
      721 cgccgaccgc tggtgcaagc gcggcctcgc ccacggcgtc gtgttccagc ccggcagcgc
      781 gttcagcctc gccggcaagc gcgtccccgc cgcccgcctg ggctacggcg cctgcaccga
      841 ggccgagatc cggctcgccg tccgccggct cgcccagtgc gcccggccgt agcgcccgtt
      901 gatgtttcat caatcccgtc tcttgacatt tcctcaatca aagacatact gctcgccatg
      961 aacgcgatcg acccggcggt cgagccggtg gggcgcaccc tgcgcacggt cgtgatcgcg
     1021 ttgctcgaag ccggccgggt ctcgctgccg gtccatgcca gcgagcggat ggatgaacgc
     1081 cacctcaccg ccccccagat cgagaccgcg ctgcgcagcg gcgcgctggc cagcgagagc
     1141 tgcgatggcg gcgcgtggcg ctaccgcgcg agcaagcaag gcgtggtcgt gatcttcacg
     1201 ttcgataccg acgacgacgg caacttgctg gtcgtcgtca ccacctggag gacccgatga
     1261 agtgcaccga gtgcggcaac aagatgaccc ggtcggtcgt cgatcacgtc tatcgtgaga
     1321 gcggcctcga caacgtcatc ctccatggga tcaccaggta caggtgcgag agctgcggcg
     1381 ccgagcgcgt gcagatcccg aacatgggcc ccctgcaccg cgccatcgcg cgggccatcg
     1441 cccacaagcc agcgcggctg gtccccgccg aggtgcgctt cctccgcgat cacctcgagc
     1501 tctcgaacaa ggacttcgcc gcgctgatgg gcgtcacccc ggagcaggca tcgcgctgga
     1561 ccagctccga gccgatcggc gtgccggccg agcgattcct ccggacgctg gccctgctcg
     1621 gacccggcgt cgtgtcgctc gaccagggcg acgaccgtga ggacgatagc ctgccggatc
     1681 ccgagctcag cccatcgctg ttcaaggcgt tgctgcgcga gcttccaccg ccgtccacag
     1741 caccgcggga ggtgccgatc gatctacggc gagctccctc gggatggaag ctggaagcgc
     1801 acgcggcgag ctgagcggat cgcggccgca cttcccgctc agcgccggct accgcgagcg
     1861 cgcgcgccgc gcctggccgc cttcaacgac cgcgccggct tgccgcgggc gggcttcgac
     1921 gacttcgcct tcggcgcctt cgcagccttc gccggctgct ccggcttccg cggcttgccc
     1981 ggccgcgccg gcctggcggg cgccggccgc tccaccggcg cgctgtggcc gagcgccacg
     2041 atccgctcgt actcggcctc ggtcaccggc tgcaccgaca gccgcatgcc acgccggagc
     2101 aacagcatgt cggccagcgc cggctcgtcc cggatccggt ccatcgtcac gaagtgcggg
     2161 aacgtcgcga cgtactcgac cgcgacgcag tcccagatcg gcgcgtcgcg cgtcgccttc
     2221 ggatcgaagt acttgctgtc gggatcgaac tgggtctcgt cgaccacccc ggtccgcatc
     2281 acgcgcgcca gccccgccac ccccggcggc ctggcattcg aatgatggaa cagcaccgcg
     2341 tcgccgaccg ccatcgcgcg catgtgggcg cgcgcgaagt agctgcgcac gccggtccac
     2401 ggctcgacgc cgacgcgctc gagatcggcg atcccgaact gatcgggctc ggacttcatc
     2461 agccagaact tgaccatctg accgaccatc gcacagctct cgccggaggt cgcgcgtcct
     2521 gcaccccgag gatgcgtggt acaatgctcc gagatggttt cgctggcttc catccccgca
     2581 ggagagtatg tgccgaccgc cgacgagcgg atcgtcatgt acaacgtgcc gtggagccac
     2641 ttcgaggtcc agctcgcgct gcgcggcgac gcctcctcgc cgcgcatggc ctatctcgac
     2701 ggagcgctgg agctgatgtc gccatccaag ggtcacgagc gaacgaagtc gtacctgggc
     2761 tgtctgatcg aagtgttcgc gatggagcgc gacctcgtgc tgtcgccgta cggcagctgg
     2821 acgctgaagg gcccacccga gctcgccggc gtcgagcccg gcgagtgcta catcgtcggg
     2881 ccggatcagg acaaggagcg tccggatctc gcgatcgagg tgttctggac cagcgggagc
     2941 atcaacaagc tggagatcta ccgccggctc aaggtcggcg aggtgtggtc ctggaaggac
     3001 ggcgtgctgt cggtgcacgt gctgtcggcg ggccgctatc agcaggtgga gcgtagcgcg
     3061 ctgttcccgg agctcgacct ggcgctgctg tgcacgttcc tcgaccgccg cagcgccacc
     3121 gaggcgatgc gcgacttccg caacgtgctg cgcgcccggc cgtagctacc ccgcgtcagt
     3181 tgctggcggc gccgatcggt gcgccgatct ccagcgtcgc caccggcacg tcgcgcgaca
     3241 cgcggtggac ctcctcggcg aatccggtca ggtcgacgtt gagcgagaac cgcagcggcg
     3301 cgtccagcgg gcggaagaag tcgtcgtagt gggtcggcac gatccgatcg ggcgccagcg
     3361 cgcgcacgat ccgctcgacg tagcgcgggg tgaaccggcg gccggcgatg ccgcacagga
     3421 acaggtcgac cccgcgctcg cggatctcgt cctcgatcag gtcggccgag ccctggtggt
     3481 agatccgcat ccccgcgacc tcgatgcaga tcccccagac ctggccgcag cggtagcgct
     3541 gcggcgtcag ctcgtcgacg tgctcgcagg tcagctcgcc ggcgtacggc acggccagcc
     3601 cgagctggag cttcgagtgc cggctcggcg cgaagcgaaa ccggaacggc ccgacctcgt
     3661 agtcgcggtg cggcgcgacg accaccgcct ggccggccag cccgtggatc ttcatcagct
     3721 gcgccagcga gctcgagccg tagacccggg caccgaaccg ccgcgcgatc gccggcacgt
     3781 cgagcgcatg atcgaagtgc gtgtggccga ccagcaccgc gtcggcgcga tcgacccacg
     3841 ccgcgatcgc ggcctccgac ggcggcacca cgtggcgcgc gacgaggtcg cgcagtgaga
     3901 gccgcgtgac gtacgggtcg atccacgcga ccgtgccctg gtacgcgagg cgaaaccccg
     3961 ccgtgccgag ccaggtcagc tccagcccgg gcggcaggct gcccgaccac gcgagctgcc
     4021 gcgcacacgt ctgatcgctg gcatgccggc ggcgcgcggt cgcg
//
LOCUS       DASVOR010002197         3866 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_374321,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002197 DASVOR010000000
VERSION     DASVOR010002197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_374321"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1470)
                     /locus_tag="VF516_40745"
     CDS             complement(<1..1470)
                     /locus_tag="VF516_40745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEX8114150.1"
                     /translation="MGRKEDALEYHSRGRRGKLEVVPTKPLVSQLDLSLAYSPGVAEP
                     CLEIARDQDRSWEYTARANLVAVISNGTAVLGLGDIGPHAGKPVMEGKACLFKKFADI
                     DVFDLEINEKDVDKFCDVVAALEPTFGGINLEDIAAPACFEIEEKLRQRMRIPVFHDD
                     QHGTAIISGAGLINAALLAGKKLEAIKLVVSGAGAAAIACVEFFCSLGMKAENVILVD
                     SKGVVHRGRKDLTRQKARWAVPDDGRRTLADAMRGTDMFMGLSIANVVTPDMLRTMSD
                     RPIVFALANPDPEISYPDAIMARPDALVATGRSDFPNQVNNVLGFPYIFRGALDCRAT
                     GVSEAMKLAAARALADLTREPVPDSVVMAYGGKSLKMGPDYFIPKPFDPRVLWWVAPA
                     VARAAMESGVARITFDITEYRERLISRGSNAAYSIMRTITKEAQRDPRRIVFPHAASP
                     RLLRAIQQIVDADIARPVLLGRPDEIEQLCVEMSLDLMSK"
     gene            1961..2034
                     /locus_tag="VF516_40750"
     tRNA            1961..2034
                     /locus_tag="VF516_40750"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1995..1997,aa:Arg,seq:ccg)
     gene            2046..2726
                     /locus_tag="VF516_40755"
     CDS             2046..2726
                     /locus_tag="VF516_40755"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114151.1"
                     /translation="MPKTLGEIAIEAGLVNKASAAKAGRLADERKLPLVVVLVRELGV
                     DEVALVGALRKQTRVPLIDPGSLEIDPEALRHVPRDVCARLRVLPMSVARDGSGKVMR
                     IAMADPTDTSAVAEIEQLTHCDVEVNALPLSAIDELVDKGYRQVNTAVVSRAGNAGAA
                     AAVPSRSRPPEAESEVSVTAQMPVATLHAMAPDDLETRLTALVNALVAKGVLTEAELA
                     EALRKLKG"
     gene            2844..>3866
                     /locus_tag="VF516_40760"
     CDS             2844..>3866
                     /locus_tag="VF516_40760"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114152.1"
                     /translation="MTATIHCAGCQHAYIATALFCPNCGRPRVREAPADPLLGKLLGE
                     RFLVQELLGQGGSGTIYRAEHVTLRRKVAIKVLHTELSRDDLAVERFRREATTVAEID
                     NEHIVEIHDFGRTPDGRLYLAMELLEGETLDTVLARDQQLSVERTADILIQVGEALME
                     AHAIGYVHRDLRPRNIYLAVRRGKANFVKLLDFGLAKLVEAEGQAASTSLGMTFGDPR
                     YMSPEQARGDRIDRRADIYQLGCVAYEMLTGAPPFGGNRVFDILTKQVTEIPQPLPTR
                     RPGVPLWMEAAVTKMLAKDPENRFATTTRMVEALRRGLETGEVMEDEIARRRESIPPP
                     SVSRVMQ"
BASE COUNT          592 a         1264 c         1446 g          564 t
ORIGIN      
        1 cttgctcatc agatcgagcg acatctcgac gcacagctgc tcgatctcgt cgggccggcc
       61 gagcaggacc gggcgcgcga tgtcggcgtc gacgatctgc tggatcgcgc ggagcaggcg
      121 cgggctcgcg gcgtgcggga acacgatgcg ccgcggatcg cgctgggcct ccttggtgat
      181 cgtgcgcatg atcgagtagg cggcgttgga gcccctggag atcaggcgct cgcggtactc
      241 ggtgatgtcg aaggtgatgc gcgcgacgcc ggactccatg gcggcccggg cgacagcggg
      301 cgcgacccac cacaggacgc gcgggtcgaa cggcttgggg atgaagtagt ccgggcccat
      361 cttgagcgac ttgccgccgt aggccatgac gaccgagtcg gggaccggct cgcgggtgag
      421 atccgcgagc gcgcgggcgg cggcgagctt catggcctcg gacacgccgg tggcgcggca
      481 gtcgagcgcg ccgcggaaga tgtaggggaa gccgaggacg ttgttgacct ggttggggaa
      541 gtcggagcgg ccggtggcga ccagggcgtc ggggcgcgcc atgatcgcgt cggggtacga
      601 gatctcgggg tcggggttgg cgagcgcgaa cacgatcggg cggtcggaca tggtgcggag
      661 catgtcgggg gtgacgacgt tggcgatcga caggcccatg aacatgtcgg tgccgcgcat
      721 cgcgtcggcc agggtgcggc ggccgtcgtc ggggaccgcc cagcgcgcct tctggcgggt
      781 caggtccttg cggccgcggt ggacgacgcc cttggagtcg acgaggatga cgttctcggc
      841 cttcatgccg aggctgcaga agaactcgac acaggcgatc gcggcggcgc cggcgcccga
      901 caccacgagc ttgatcgcct cgagcttctt gccggcgagc agggcggcgt tgatcaggcc
      961 cgcgcccgag atgatcgcgg tgccgtgctg atcgtcgtgg aacaccggga tccgcatgcg
     1021 ctggcgcagc ttctcctcga tctcgaagca cgccggcgcg gcgatgtcct cgaggttgat
     1081 gccgccgaac gtcggctcga gcgcggcgac gacgtcgcag aacttgtcga cgtccttctc
     1141 gttgatctcg aggtcgaaca cgtcgatgtc ggcgaacttc ttgaacaggc aggccttgcc
     1201 ttccatcacc ggcttgccgg cgtgcgggcc gatgtcgccg aggccgagca cggcggtgcc
     1261 gttggagatc acggcgacca ggttggcgcg cgcggtgtat tcccacgagc ggtcctggtc
     1321 gcgtgcgatc tccaggcatg gctcggcgac gccggggcta taggcgagcg agagatcgag
     1381 ctgactgacc agcggcttgg tcggtacgac ctcgagcttt cctcggcgtc cacggctgtg
     1441 atattcgagc gcgtcttcct ttcgccccat gcagcgcacg gtagacctgt cgcgcacggc
     1501 tggcaaatca caccagaccg gtctcgaaaa tccggggatc aggggcactg aacttggtgg
     1561 gcaatcacac cgcctccggt cgcgcgcctg cgacccgtcg ttcgtgggag cgcggtgaaa
     1621 cggtgtttca cggatcgctc ggcgcgatgg tcgcccggcg cacgctgtgt cggaactccg
     1681 acagcgcctg ccgcggcgtc gcggccgagc gcgttgcctc gggtgctgca gcgtgccgca
     1741 gatcctgcgc ggcgcatgcc gcgggcggga acgcggggcg agcaggggcg gaggcgggct
     1801 cgtcgagccc tggcaccgat cgcgcttttc gcgggcgggc ggacctatcg tcgagcgccg
     1861 ggcggcgatg gcccccgaca gcgcccaccc ggcggtggct cggtaacctg gccctccgac
     1921 acttgtttct cgcgaccgcg cggagtagaa agctcagcac gcacccgtag ctcagctgga
     1981 tagagcgttg gcctccggag ccaaaggtca caggttcgaa tcttgtcggg tgcacgcaga
     2041 gatccgtgcc aaaaacgctg ggagagatcg cgatcgaggc cggcctcgtc aacaaggcca
     2101 gcgctgcgaa ggcgggccgg ctggccgacg agcgcaagct gccgctggtg gtcgtgctgg
     2161 tgcgcgagct cggggtcgat gaggtcgcgc tggtcggggc gctgcgcaag cagacccgcg
     2221 tgccgctgat cgatccgggg agcctggaga tcgaccccga ggcgcttcgc cacgtcccgc
     2281 gcgatgtgtg cgcccggctg cgggtgctgc cgatgtcggt ggcgcgcgac ggcagcggca
     2341 aggtgatgcg gatcgcgatg gccgacccga ccgacacctc cgcggtcgcc gagatcgagc
     2401 agctgacgca ttgcgacgtc gaggtcaacg cgctgccgct gtcggcgatc gacgagctgg
     2461 tcgacaaggg ctatcgccag gtcaacacgg cggtggtgag ccgcgcgggc aacgcgggag
     2521 cggcggcggc ggtcccatcg cgcagcaggc ccccggaggc cgaatcggag gtctcggtca
     2581 cggcgcagat gccggtcgcg acgctgcacg cgatggcgcc cgacgacctg gagacccggc
     2641 tgaccgccct ggtcaacgcg ctcgtcgcca agggcgtcct gaccgaggcg gagctggccg
     2701 aggcgctgcg caagctgaag ggctgaacgg agcgacagcg acgaatccgg ccgtggcggc
     2761 gtttgccacg ggcatggccc gaggccgtgc gctagtattc gggccggtcg tccgtccgcc
     2821 ccacgaccat gcccgcacat gacatgactg cgacgatcca ctgcgccgga tgtcaacacg
     2881 cgtacatcgc gaccgcgctg ttctgcccga actgcggtcg gccgcgagtg cgcgaggcgc
     2941 cggccgatcc gctgctcggc aagctgctcg gcgagcggtt cctggtccag gagctgctcg
     3001 ggcagggcgg atccggcacg atctaccgcg ccgagcacgt gacgctgcgc cgcaaggtgg
     3061 cgatcaaggt gctgcacacc gagctgtcgc gcgacgacct cgcggtcgag cggttccggc
     3121 gcgaggcgac gacggtcgcc gagatcgaca acgagcacat cgtcgagatc cacgacttcg
     3181 ggcggacgcc cgacggacgg ctctacctcg cgatggagct gctcgagggc gagacgctcg
     3241 acaccgtgct ggcgcgcgac cagcagctgt cggtcgagcg caccgccgac atcctgatcc
     3301 aggtcggcga ggcgctgatg gaagcgcacg cgatcggcta cgtccaccgc gacctgcggc
     3361 cgcgcaacat ctacctcgcg gttcgccgcg gcaaggcgaa cttcgtcaag ctgctcgact
     3421 tcgggctggc caagctggtc gaggccgagg gccaggcggc gtcgaccagc ctcggcatga
     3481 cgttcggcga tccgcgctac atgtcgcccg agcaggcgcg cggcgaccgg atcgaccgcc
     3541 gcgccgacat ctaccagctg ggctgcgtcg cctacgagat gctgaccggg gcgccgccgt
     3601 tcggcggcaa ccgggtgttc gacatcctga ccaagcaggt gaccgagatc ccgcagccgc
     3661 tgccgacccg gcggcccggc gtgccgctgt ggatggaagc ggcggtcacc aagatgctcg
     3721 ccaaggaccc cgagaaccgg ttcgcgacca cgacccggat ggtcgaggcg ctgcgccgcg
     3781 ggctcgagac cggcgaggtg atggaggacg agatcgcgcg ccgccgcgag agcatcccgc
     3841 cgccgtcggt gtcgcgggtg atgcag
//
LOCUS       DASVOR010002198         2942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_344465,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002198 DASVOR010000000
VERSION     DASVOR010002198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344465"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="VF516_40765"
     CDS             complement(<1..896)
                     /locus_tag="VF516_40765"
                     /inference="COORDINATES: protein motif:HMM:NF014630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HEX8114153.1"
                     /translation="MREQLVLLLQLQQSDSKVRELKTAVTQLPTKLDPLRRDLAKLQT
                     MLDGERSKLNETNSWRKAQQEMIDREREALRQAQGKFQASKNTKEFNASSREVENKRK
                     AIGDRESELKKVNEAAAQSSAQLEARTRDVDALRAELATSEATMASQLDALKAQLAEA
                     TAARDAARVQVDTQWVKIYDSLAAKKGYAVAPVVKGVCRGCHMTLPPQLANMLVRMET
                     IETCPRCGRLIYRKELLEPEPAPEAATEAKAEAAARPKPEPKRARTGAKGGRTKGEKK
                     PEAASEARVEAQADAASETRSEA"
     gene            complement(979..1051)
                     /locus_tag="VF516_40770"
     tRNA            complement(979..1051)
                     /locus_tag="VF516_40770"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1016..1018),aa:Ile,seq:cat)
     gene            1196..1819
                     /locus_tag="VF516_40775"
     CDS             1196..1819
                     /locus_tag="VF516_40775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114154.1"
                     /translation="MSAGNVARVVAVLLACAAAAGSATSTSSSNQQPLAGPGSDSSPP
                     PPQQMEPARALGLWRSTFGAVKIEPDDSKGGLDAGNLHGIWVYQRQGQEVIGYFAGNL
                     RGNVLQFRWQEPNDPPLTGEGFLVFDVQGRQYSGRWWSDRRDRVGDWSGWRQGAPMAR
                     PVDATSPYGGQGYGEPEGTYRPRRPQGYQPYRPQSSQPQAQPQQTYF"
     gene            complement(1849..2733)
                     /gene="dapF"
                     /locus_tag="VF516_40780"
     CDS             complement(1849..2733)
                     /gene="dapF"
                     /locus_tag="VF516_40780"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEX8114155.1"
                     /translation="MRFAKYHGLGNDFLVVDLRTADPADAAAIQAPRNVIALCDRQFG
                     VGGDGVLAILPSATADARMRVLNSDGSEAEMCGNGIRCVARELYERGGLARPALAIDT
                     GAGRLVCEIDADGGAVRSVTVAMGIPRLLRGEIPMTGPAGERCVEQPLVTAGAPRPVT
                     CVSMGNPHAVTFVGSRDEAMELARTVGPAVERHAWFPNRTNAEFAHVKQRREIDLVVW
                     ERGCGITLACGTGACATAVAAIVTGRADEATPIRVNLPGGSLEITVHSGLSAVSMKGP
                     ALHVFDGELDLARLQPRP"
BASE COUNT          354 a         1028 c         1101 g          459 t
ORIGIN      
        1 gcctcggacc gcgtctcgct cgccgcgtcg gcctgcgctt cgacccgggc ctcgctcgcc
       61 gcctcgggct tcttctcgcc cttggtgcgg ccgcccttgg cgccggtcct ggcgcgcttg
      121 ggctcgggct tgggccgggc cgccgcctcg gccttggcct cggtcgccgc ctcgggcgcg
      181 ggctcgggct cgagcagctc cttgcggtag atcagccggc cgcagcgcgg gcaggtctcg
      241 atggtctcca tccggacgag catgttggcg agctgcggcg gcagggtcat gtggcagccc
      301 cggcacacac ccttgaccac cggggcgacc gcgtagccct tcttggcggc gagcgagtcg
      361 tagatcttga cccactgcgt gtcgacctgg acccgggcgg cgtcgcgcgc ggcggtcgcc
      421 tcggcgagct gggccttgag cgcgtcgagc tggctcgcca tcgtggcctc gctcgtggcg
      481 agctcggcgc gcagcgcgtc gacgtcccgg gtgcgggcct cgagctgggc gctcgactgg
      541 gcggcggcct cgttgacctt cttgagctcg ctctcgcgat cgccgatcgc cttgcgcttg
      601 ttctcgacct cgcggctgga cgcgttgaac tccttggtgt tcttcgacgc ctggaacttg
      661 ccctgcgcct ggcgcagcgc ctcgcgctcg cgatcgatca tctcctgctg ggccttgcgc
      721 cagctgttgg tctcgttcag cttgctgcgc tcgccatcga gcatggtctg gagcttggcg
      781 aggtcgcggc gcaatggatc gagcttggtg gggagctggg tgactgcggt ctttagttcg
      841 cggaccttgc tgtccgactg ctggagctgg aggaggagga cgagctgttc gcgcacttcg
      901 taccctttcg gcgtgccatc tttgcatggc cgcggcatgg ccgcgaggat cgccccgtcc
      961 ggggcttcgt cttgtcagtg ggcccacctg ggtttgaacc agggacccac cggttatgag
     1021 ccggccgctc taaccgctga gctatgggcc cgcgccccga gggtgccagc tgggtgccag
     1081 ctggtgtgga gcaccgcacg gattgcaggc gcacactatc aggttgggag aaatcgacaa
     1141 cacctggcgc acgatccccg gctgcggcga acggcgcgcg ggtatactac gttcgatgag
     1201 cgccggtaac gttgcgcggg tcgtcgccgt cctgcttgcg tgcgccgcgg cggcagggtc
     1261 cgcgacgtcg acgtcgagct ccaaccagca gccgctcgcc gggcccggga gcgatagcag
     1321 cccgcccccg ccgcagcaga tggagcccgc gcgggcgctc gggctgtggc gctcgacgtt
     1381 cggcgcggtg aagatcgagc ccgacgacag caagggcggg ctcgacgccg gcaacctgca
     1441 cggcatctgg gtctaccagc ggcagggcca ggaggtcatc ggatacttcg cgggcaacct
     1501 gcgcggcaac gtgctgcagt tccgctggca ggagcccaac gatccgccgc tgaccggcga
     1561 gggcttcctg gtgttcgacg tccagggccg gcagtactcg ggccgctggt ggagcgaccg
     1621 gcgggaccgc gtcggggact ggagtggctg gcggcagggc gccccgatgg cgaggccggt
     1681 cgatgcgacg tcgccctacg gcgggcaggg ctacggcgag ccggagggca cgtatcgccc
     1741 acgtcggccg cagggctacc agccataccg gccgcaatcg tcgcagccgc aggcgcagcc
     1801 gcagcagacg tacttctagc cccccgcgcg agtcagaggg gtcccgggct acggccgcgg
     1861 ctgcagccgc gcgaggtcga gctcgccgtc gaacacgtgg agcgccgggc ccttcatcga
     1921 cacggcgctg agccccgagt gcacggtgat ctccaggctg cctccgggga ggttgacccg
     1981 gatcggggtg gcctcgtcgg ctcgcccggt gacgatcgcc gcaaccgcgg tggcgcacgc
     2041 gccggtcccg caggccaggg tgatgccgca gccgcgctcc cacacgacga ggtcgatctc
     2101 ccgtcgctgc ttgacgtgcg cgaactcggc gttggtgcgg ttggggaacc aggcgtggcg
     2161 ctcgaccgcc ggcccgacgg tccgggcgag ctccatcgcc tcgtcgcgcg agccgacgaa
     2221 ggtgacggcg tgggggttgc ccatcgagac gcaggtcacc gggcggggcg cgccggcggt
     2281 caccagcggt tgctcgacgc agcgctcgcc ggcgggcccg gtcatcggga tctcgccgcg
     2341 caggaggcgc gggatgccca tcgcgacggt caccgaccgc acggcgccgc cgtcggcgtc
     2401 gatctcgcag accagccggc cggcgccggt gtcgatcgcg agcgcgggcc tggcgaggcc
     2461 gccgcgctcg tagagctccc tggcgacgca gcggatcccg ttgccgcaca tctcggcctc
     2521 gctgccatcg ctgttgagca cgcgcatccg cgcatcggcg gtggccgacg gcaggatcgc
     2581 gagcacgccg tcgccgccga cgccgaactg gcgatcgcac agggcgatca cgttgcgcgg
     2641 cgcctggatg gcggccgcgt cggcggggtc ggcggtgcgc agatcgacca cgaggaagtc
     2701 gttccccagg ccgtggtact tggcgaaccg catgcgggga atctagcaga tcgcccggca
     2761 gatccgcccg ggctcgctgc gcgcgcggcc acgaggtcgg aacgccgcgg ggggatcgcc
     2821 accgcgggcg atcacttcgg cttggcggcc gacccggcgg gcttcgcagc ggcgtcgggc
     2881 ttcgcggcgt cgggattggc accctcagcc ggcttcgcgg gatcggccgg cttcgcggtg
     2941 tc
//
LOCUS       DASVOR010002199         1978 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_199112,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002199 DASVOR010000000
VERSION     DASVOR010002199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199112"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..638)
                     /locus_tag="VF516_40785"
     CDS             complement(51..638)
                     /locus_tag="VF516_40785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8114156.1"
                     /translation="MAAAPPSDDAEDEVLVAAMAAGDRAALATLYERHGSLLLGLALR
                     IVRERREAEDLLHDVFLEAWRTAKDFDPKRGRVRTWLAIRMRSRALDLQKSARVSRNT
                     GDGGLELLVDEGDAASPDHGRVRRALAELGTDQRRVLELAYFEGLSCTEIAERVAIPV
                     GTVKSRIAAGLDRLRSGLLSAGAGGPPGIPRSEKT"
     gene            696..>1978
                     /locus_tag="VF516_40790"
     CDS             696..>1978
                     /locus_tag="VF516_40790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114157.1"
                     /translation="MLEALRDAVHAKATERTWSQGVALARDGRVVGRKPAAGNGDIEL
                     EVRVPGRPTPFEVMLYPAHGDWECSCDASPVCSHVVAGVLSAVNAAKTGGAVPDDGAR
                     GLATIRYLLIPAPGGVLVDRVLVRKDRDRTEPLAGSLLSLVGTGKASAIATTEADLTA
                     DQLITARGGPIHGDRLDRLLEVLADARDVRWKGEPVTTGREPVLPRAVIVDAPGGVEV
                     RIEADPVVREVVAVGVVRTTDDVLRPIGAIDYSGPRLDKLPLVQHVPQAAFPTLLGVT
                     LPELERRLTVEIRATGLPKLGHREPPRMAFDVKPDGDRLWVLPTLVYGDPPRARVDGN
                     NLVHLEGPLPIRDLDAERKLIHRLRDELNLVPGRRVELSGKEASQTRNKLTAWLRSDT
                     LAANPSRAIDLDIQISIDGMRVEAALSDGERSVPI"
BASE COUNT          280 a          730 c          697 g          271 t
ORIGIN      
        1 atccaggata ccgagcgcgt acagcggcag gagctcacga acgtcgccgg tcatgtcttc
       61 tccgatcgag ggattccggg gggtccgccc gcgccggcgc tgagcagccc ggagcgcagg
      121 cggtccaggc ccgcggcgat acgcgacttg accgtgccga ccgggatcgc gacccgctcg
      181 gcgatctcgg tgcacgacag accctcgaag tacgcgagct ccagcacccg gcgctggtcg
      241 gtgccgagct cggcgagcgc ccggcgcacc cggccgtgat cggggctcgc ggcgtccccc
      301 tcgtccacca gcagctcgag cccgccatcg ccggtgttgc gcgacacgcg tgccgacttt
      361 tgcaggtcca gggcccggga acgcatacgg atcgctaacc aggtacgaac ccgaccgcgc
      421 ttcggatcga agtccttggc ggtccgccag gcctcgagga acacatcgtg caagagatcc
      481 tccgcctcgc gcctctcccg gacgatgcgc agcgccagcc cgagcaacag cgaaccgtgg
      541 cgctcgtaca gtgtcgccag agctgcgcga tcaccggcag ccatcgccgc gacgagcacc
      601 tcgtcttcgg cgtcgtcgga cggcggggcg gcagccatgt gcccgagagg ctagcacgcc
      661 tttcgtgtcg agtgcttgcg tgctaatcac ccgcggtgct cgaagcgctt cgcgatgcgg
      721 ttcacgcgaa ggccacggag cgcacctggt cacagggcgt cgcgctggcc cgggacggcc
      781 gcgtggtcgg ccgtaaaccg gcggccggca atggtgacat cgagctcgag gtccgcgtac
      841 ccggccggcc gacgccgttc gaggtgatgc tgtacccggc gcacggcgac tgggagtgct
      901 cctgcgacgc cagccccgtg tgctcccacg tcgtggccgg cgtgctgtcc gcggtcaacg
      961 ccgcgaagac cggtggcgcg gttcccgacg acggcgcgcg cggcctggcc acgatccgct
     1021 acctcttgat cccggccccg ggcggcgtgt tggtcgatcg cgtgctggtg cgcaaagatc
     1081 gggatcgtac ggagccgctc gccgggtcgc tgctgtcgct ggtcggcacc ggcaaggcgt
     1141 cggcgatcgc gacgaccgag gccgacctca cggccgacca gctgatcacc gcgcgcggcg
     1201 gtccgatcca cggcgaccgc ctcgaccgcc tgctcgaggt gctcgccgac gcccgcgacg
     1261 tgcgctggaa gggcgagccg gtcaccaccg gccgtgagcc ggtcctcccg cgcgccgtga
     1321 tcgtcgatgc gcccggcggc gtggaggtcc ggatcgaggc cgacccggtg gtccgcgagg
     1381 tggtcgcggt cggtgtcgtg cgcaccaccg acgatgtgct gcggccgatc ggcgcgatcg
     1441 actacagcgg gccgcggctc gacaagctgc cgctggtgca gcacgtcccg caggcggcgt
     1501 tcccgacgct gctcggcgtg accctgcccg agctcgagcg ccgcttgacc gtcgagatcc
     1561 gcgcgaccgg cctgcccaag ctcggccacc gcgagccgcc gcggatggcg ttcgacgtca
     1621 agcccgacgg cgaccggctg tgggtgctgc cgaccctggt ctacggcgat ccgccgcgcg
     1681 cccgggtcga cggcaacaac ctcgtccacc tcgagggtcc gctgccgatc cgcgacctcg
     1741 acgccgagcg caagctgatc caccggctgc gcgacgagct caacctcgtg ccgggccgcc
     1801 gcgtcgagct gtccggcaag gaggcgtcgc agacccgcaa caagctcacc gcgtggctgc
     1861 gcagcgacac gctcgccgcc aatccgtcgc gggcgatcga tctcgacatc cagatctcga
     1921 tcgacggcat gcgggtcgag gccgcgctgt cggacggcga gcgcagcgtg ccgatcga
//
LOCUS       DASVOR010002200         7628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_112277,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002200 DASVOR010000000
VERSION     DASVOR010002200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112277"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1460)
                     /locus_tag="VF516_40795"
     CDS             complement(<1..1460)
                     /locus_tag="VF516_40795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114158.1"
                     /translation="MVAVTNAYCERLGICVPSLAATRVHPEANTYALLIVTLLEHGRP
                     MTLEEVALRFEAAGVACADDAYIALKRCRPARPPVFRDGELYALDPHDDELDLWAFRL
                     GLRPPRVPRREPPRPPPRPPASQRLSIDELDTAWRTDVSLMGWSAQRIALAILDAHDR
                     PMQPDEVVAFVAARTRWHKLVPGPTTFRRAGAAIAIGADGAWSIVPGAPELVMARDTV
                     RDAVERVHRYPVRSTPEQIEESRRAAEKRRAAHADELAVLRRVIVHAFPARSPRIVVL
                     VDVEQRELATYLDEQLADLGPVLARHEVLCGVDIRATLRELAIDPGDRRLAELGPPQK
                     SIRLGRSGRTLKITTAMLVQGSCGIRRPFGDPDQLHAYLASGQIMQLQRRLEADAQSL
                     LALHQYGKLHGCVRLRRGSLDRYFPVPWHHRDEPTLYHLKREAHRLAMGIIAVVGSAP
                     GWEDPWSLARRLDVLRGASEYELMLFDELGGYVDDR"
     gene            complement(1686..3389)
                     /locus_tag="VF516_40800"
     CDS             complement(1686..3389)
                     /locus_tag="VF516_40800"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8114159.1"
                     /translation="MLAVVAQPKLARGGLPPGMGALRVSGLCRTVQPHARERITVTPV
                     PPGQLRIVGQGRPLEPGLRGAIERLLHADLSGVRVHEGPAAPAMGALAFTVGDDIHFA
                     PGRFDPSTRDGVALLGHELTHVVQQREGRVANPYRSGLVIVQDPALEAEADRVGRWIA
                     EQVGSRRAAGGATAGKVAPWPGSAPLRLAPRAAAVQAMMQDSPLLRRIDFTSARHAET
                     DRNAMREILQIPFYARVVQELLDRRDDMIIRRYDLTITDTRVTDRTNRALWDPNARQI
                     RLRPEVEDGATRRELIKWELLNGWRRFDASGVNAQTGPVGYADLVETFEYGTAKAFAE
                     NWNDRDGLTFAWAPTLKAALREHREMSTHDWVTSMGRTTGDAHYRNAQERYLHAHGGA
                     YLQTPGGLQWLASRVGTWFLETETWRGLDPSARGVIERGLRVEQERLARERDQQDQEI
                     AELLRQRRAAAKAQAPVPGGRGTVVRQHERRDSPSPPPRRRINYIDVSVRESGTYRDR
                     YVINSITIIYDDGSRSYPALNDGPLDRYYPDNRAIERDVAALYGVPQRDVAIRRHREL
                     R"
     gene            complement(3706..4779)
                     /locus_tag="VF516_40805"
     CDS             complement(3706..4779)
                     /locus_tag="VF516_40805"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX8114160.1"
                     /translation="MAMPQTPTFADTEVTVCSEGDFLLFLRAQRPGCGSLDWFREHRQ
                     AVMAALDHYGAVFFRGFEVDTRQFQDALDILKPERYDMIDIVTPRSHVRDTLYTSTQA
                     HPAIDIPLHQEMPYRLYPPWYLGFFCEVPSVGDGFTPAYDQRKLGRTVRRLFPAVLDR
                     FIELGVRYVRNFNQYSFKSWQTCWETTSRSEVEARLRKDDTEFEWVEGDWLRTFQRRP
                     AVLRDPISKAEILFPSFGIFHHTFIAHIADKMGLTPPPSGAEQALACEFGDGSPIPED
                     FIAWVHEEVVRTRAMIPWQPGDFFLLSNLIAGHGRTPYTNPGRTLHASFRGRLDLRAL
                     AVARAPGGHEVAREPGRPANELA"
     gene            complement(4790..5347)
                     /locus_tag="VF516_40810"
     CDS             complement(4790..5347)
                     /locus_tag="VF516_40810"
                     /inference="COORDINATES: protein motif:HMM:NF022318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FcoT family thioesterase"
                     /protein_id="HEX8114161.1"
                     /translation="MTATPERWEMLPDRLARVLVPYRNNCKYLQRIAFEYLPTSADVS
                     ADQSEASLTAIGEMSIAESCYIDSTGHFNAVEFQICANQLMYALGAECTRLRLVRALA
                     ALDLDGFFERQLANVLITNVTSTFRRVVNPRRFRGRLQLDRSYLMRERLFWECTVTYS
                     DDENGLADGNIVVTMGLGDKMAPKE"
     gene            complement(5344..6210)
                     /locus_tag="VF516_40815"
     CDS             complement(5344..6210)
                     /locus_tag="VF516_40815"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX8114162.1"
                     /translation="MQIIPGASGVLGAQIHGIDLGGITSEQARDVLAAVHHHALVRFK
                     NQKMTVHQYVALGEKLGTIMPYPDERYRHPEHPGVFVSSNVKDRTMGMARSGYFWHTD
                     LSFKPRPQPLTVLYPQLLPQAKRETLFINMATIYDALPAALRSIVDGRTAKHNPALRY
                     KVSHADVEANRDIGDLLEMGRRLYPPPTHPLVITHPVTRRRSLYMNSGFTTSIDGIPY
                     EESQAALVELFAFIERPEHVQPHSWELGDIIIWDNRTLIHKAGYMAPDDFQTIFRLGI
                     DDQVPFYDGIAA"
     gene            complement(6243..7079)
                     /locus_tag="VF516_40820"
     CDS             complement(6243..7079)
                     /locus_tag="VF516_40820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114163.1"
                     /translation="MNIEPTAFETNIHDGGLFYRLFDEAEAVRWKMADCGWPELRPAD
                     VSPAWRRVARETLVGELTTFSATERFLADFGDDVDFTQWLTVWLYEETKHPSAVLKWL
                     DALGEKFDSRFMLQGRETIPFMPSKMGTLATNVISEMVAAAVYLTISAQPTCEPVLKQ
                     IYRNIGGDEARHAASFYSYAKRRLGRTDDPKSEQLAALKVLYFWTTPELNARVGHPVN
                     MLANRYRSIPEIQEAVPHQAMASAVDNAYRRACRVFESLLGLELRDVRDVGDQIAALQ
                     AS"
     gene            complement(7076..7291)
                     /locus_tag="VF516_40825"
     CDS             complement(7076..7291)
                     /locus_tag="VF516_40825"
                     /inference="COORDINATES: protein motif:HMM:NF024763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEX8114164.1"
                     /translation="MSTTYSIAGDCLRCGACSSLAPGIIAMGETSAVIVRQPATAAEI
                     AATEAALFNCPMLAIRKRTASVQEVLR"
     gene            complement(7288..>7628)
                     /locus_tag="VF516_40830"
     CDS             complement(7288..>7628)
                     /locus_tag="VF516_40830"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEX8114165.1"
                     /translation="VAAPAAAARPAPAPAAAPSLRDRLDAAPPAERASLLLGLVRTEV
                     AAVLKLPDPGALHEDRPFEELGADSLMAMDIRFRLERRLSTKLPATLAFDHPTCRSLV
                     QYLLTLWSAS"
BASE COUNT         1159 a         2590 c         2659 g         1220 t
ORIGIN      
        1 tcgcggtcat ccacgtatcc gccgagctcg tcgaacagca tcagctcgta ctcgctcgcg
       61 ccgcgaagaa catcgaggcg acgcgcgaga gaccacggat cctcccagcc gggcgccgag
      121 cccacgaccg cgatgatgcc catcgccagc ctgtgggcct cgcgcttgag atggtacagc
      181 gtcggctcgt cgcgatggtg ccagggcacc gggaaatatc gatcgagaga gccccggcgc
      241 aggcgaacgc agccgtgcag cttgccgtac tgatggagcg cgagcagcga ctgcgcgtcg
      301 gcctcgagcc ggcgctgcag ctgcatgatc tgaccgctcg cgagatacgc gtgtagctgg
      361 tccgggtcgc cgaacggtcg acggatgcca cacgagccct ggaccagcat cgcggtcgtg
      421 atcttcaacg tgcggccaga gcggccgagc cggatcgact tctgcggtgg ccccagctcg
      481 gcgagccgcc gatccccggg gtcgatcgcg agctcgcgca gcgtcgcgcg gatgtcgacg
      541 ccgcacagca cctcgtgacg cgcgagcacg gggccgaggt cggcgagctg ttcgtcgagg
      601 tacgtcgcga gctcgcgctg ctcgacatcg acgagcacca cgatgcgcgg ggagcgcgcg
      661 ggaaacgcgt gcacgatcac gcgccggagg acggcgagct catccgcatg cgcggcacgt
      721 cgtttctcgg cggctcgccg gctctcctcg atctgctcgg gagtggagcg aaccgggtaa
      781 cgatgcaccc gctcgaccgc gtcgcgcacg gtgtcacgcg ccatgacgag ctcgggtgcg
      841 cccggcacga tcgaccacgc gccgtccgcg ccgatcgcga tggcggcccc cgcacgacgg
      901 aacgtcgtcg gcccgggcac cagcttgtgc cacctggtgc gtgcggccac gaacgcgacc
      961 acctcatccg gctgcatcgg tcggtcgtgt gcgtcgagga tcgcgagcgc gatccgctgc
     1021 gccgaccagc ccatgaggct gacgtcggtt cgccacgccg tgtcgagctc gtcgatgctc
     1081 agccgctgac tcgcgggtgg ccgcggagga ggtcgcggcg gctcgcgccg cgggacgcgc
     1141 ggcggccgga gcccgaggcg gaacgcccac aggtcgagct cgtcgtcgtg cggatcgagc
     1201 gcgtagagct cgccgtcgcg aaacaccggg ggccgcgcag gccggcagcg cttgagcgcg
     1261 atgtacgcat cgtccgcgca cgcgacgcct gcggcctcga acctcagcgc gacctcctcg
     1321 agcgtcatcg gccggccgtg ctcgagcaac gtgacgatca ggagcgcata ggtgttcgcc
     1381 tcgggatgca cgcgcgtcgc cgcgagcgag ggcacgcaga taccgaggcg ctcgcagtag
     1441 gcgttggtga cggccaccat cgtgcgcggt gtaccagaac caggcgacgt gatcgagccc
     1501 gagctggctg tcagcccgtc cccgtggagt ctccgcgtga cgtacacgaa tcagcccaag
     1561 gcgacgctgc gggggacgaa gcccccgatg ttccccgaga tgcatcgagg tcgctcgtga
     1621 tcggcccgat cgcgcgtggt ctacgctcgg tggtctcgct caggacctcg aggatgaccc
     1681 gaggttcaac gcagctctcg gtgtcgccgg atcgcgacgt ctcgttgcgg cactccatag
     1741 agtgctgcca cgtcccgctc gatggcccgg ttgtcaggat aatatcggtc cagaggacca
     1801 tcgttcaagg ctgggtagct ccgcgaccca tcgtcataga tgatcgtgat gctgttgatc
     1861 acgtatctgt ctcgatacgt cccgctctcc ctcacggaga catcgatgta gttgatgcgg
     1921 cggcggggcg gcggggaggg cgagtcgcgc cgctcgtgct gccggaccac cgtcccacgc
     1981 ccacccggca cgggggcctg cgcctttgcg gcggcgcgcc gctggcggag caactccgcg
     2041 atctcctggt cctgttggtc ccgctcgcgg gccagacgtt cttgctcgac gcgcagaccg
     2101 cgctcgatca cgccgcgggc cgaggggtcg agaccgcgcc aggtctcggt ctccaggaac
     2161 cacgttccta cccgcgaggc gagccactgc agccccccgg gcgtctgcag atacgcgcca
     2221 ccatgcgcat ggagatagcg ctcctgggcg ttgcggtagt gagcatcgcc ggtcgtcctt
     2281 cccatggaag ttacccagtc gtgggtgctc atctctcggt gctcgcgcag cgccgccttg
     2341 agcgtcgggg cccacgcgaa ggtcagcccg tcgcggtcgt tccagttctc ggcgaaggcc
     2401 ttcgcagtac cgtactcgaa ggtctccacg agatcggcat acccgacggg cccggtctga
     2461 gcgttcacgc ccgacgcatc gaagcggcgc cagccgttga gcagctccca cttgatgagc
     2521 tcccgccggg tcgcgccgtc ctcgacctcg ggccgcagcc ggatctggcg ggcgttgggg
     2581 tcccataacg cgcgattggt gcggtcggtg acccgcgtgt ctgtgatggt cagatcgtag
     2641 cgccggatga tcatatcgtc tcgacgatcg agcagttcct ggacgacgcg cgcatagaac
     2701 gggatctgga gaatctcccg catcgcgttc ctgtcggtct cggcgtgccg tgccgaggta
     2761 aagtcgatcc gccgcagcaa cggactgtcc tgcatcatgg cctggaccgc agccgctcgc
     2821 ggcgcgagcc ggagaggtgc cgagcccggc catggcgcta ccttgccggc cgttgcgccc
     2881 ccggccgctc ggcgcgaccc cacttgctcc gcgatccagc gccccacccg atccgcttct
     2941 gcctccagcg caggatcctg cacgatcacg agcccggatc ggtaggggtt tgccacccgg
     3001 ccctcgcgct gctgcacgac atgcgtcagc tcgtggccga gcagcgcgac gccgtcgcgg
     3061 gtcgacggat cgaagcgccc cggggcgaag tgaatatcgt caccgacggt gaacgccagc
     3121 gcgcccatgg ctggcgccgc cggtccctcg tgcacccgca cgcccgagag gtccgcgtgc
     3181 aacagccgct cgatggcgcc gcggagcccc ggctcgaggg gacggccctg gcccacgatc
     3241 cggagctggc ccggcggcac cggcgtcacc gtgatccgct cgcgggcgtg tggctgcacc
     3301 gtccggcaca gcccgctcac gcgaagcgcg cccatccccg gaggcaagcc gccccgtgcg
     3361 agcttgggct gggcgacgac cgccagcatc ttcgcgggca gcagatcggg ccgcggtacg
     3421 ccggaaggcg cgggcgccgt cccgcgcggt ggggcggccc cgcccgcgcg aaacggctcg
     3481 atctggaagt agccctgcag taccaggccg gcgctcgccg cggtcttgtt cgctgtcacg
     3541 gctcctggat ccctgtcgcg cgactctatc gcccaggtcc gttcccggct aatttttgcc
     3601 ggaccgagcg gtgcggtgcg gcatatgccc gatgtgcaat gagcgggaga tgacgaaggc
     3661 ccgaccgacg cgcgatgccg gtagtgcgcg acctttgccc ccggttcagg ccagctcgtt
     3721 cgcagggcga cccggctcgc gcgcgacctc gtgcccgccc ggcgcgcggg cgaccgcgag
     3781 cgcgcggaga tcgaggcgtc cccgaaacga cgcgtggagc gtgcggcccg gattcgtgta
     3841 cggcgtcctg ccgtgccccg cgatcaggtt gctgagcagg aagaagtcgc cgggctgcca
     3901 ggggatcatc gccctcgtcc tgaccacctc ctcgtgcacc cacgcgatga agtcctcggg
     3961 gatcggcgag ccatcgccga actcgcacgc cagcgcctgc tcggccccgc tcggcggcgg
     4021 cgtcaagccc atcttgtcgg cgatgtgcgc gatgaacgtg tgatgaaaga tgccgaacga
     4081 cggaaacagg atctccgcct tgctgatcgg atcgcgaagc acggccgggc ggcgctggaa
     4141 cgtccggagc cagtcgccct cgacccactc gaactcggtg tcgtccttgc gcagcctggc
     4201 ctccacttcg ctgcggctgg tggtctccca gcacgtctgc cacgacttga agctgtactg
     4261 gttgaagttc cggacgtacc tcacgccgag ctcgatgaac cgatcgagca cggccggaaa
     4321 caggcgccgg acggtacggc cgagcttccg ctggtcgtac gccggcgtga acccgtcccc
     4381 cacggagggg acctcgcaga agaacccgag gtaccacggc ggatacaggc gatacggcat
     4441 ctcctgatgc agcgggatat cgatcgccgg atgcgcctgc gtcgacgtgt acagcgtgtc
     4501 gcgaacgtgg gagcgcggcg tgacgatgtc gatcatgtcg tagcgctctg gcttgaggat
     4561 atccagcgcg tcctggaact gccgggtatc gacctcgaac ccgcgaaaga acacggcgcc
     4621 gtaatgatcg agcgccgcca tgaccgcctg acggtgttcg cggaaccagt cgagcgatcc
     4681 gcacccgggg cgttgtgccc gcaggaacag gaggaaatcg ccctcgctgc aaaccgtcac
     4741 ctcggtgtct gcgaacgttg gagtctgtgg catcgccatg ttgtgtctcc tactccttgg
     4801 gggccatctt gtcgccgagc cccatcgtga cgacgatgtt gccatcggcc aatccattct
     4861 cgtcatcgga gtacgtgact gtgcactccc agaacagtcg ctcgcgcatg agatacgagc
     4921 gatcgagctg caggcggccg cggaatcgcc gcgggttgac cacccggcgg aacgtgctcg
     4981 tcacgttcgt gatcagcacg ttggcgagct ggcgctcgaa aaatccgtcc aggtccagcg
     5041 ccgcgagcgc gcgcacgagg cgcagccggg tgcactcggc gccgagcgcg tacatcagct
     5101 gattcgcgca gatctggaac tcgacggcgt tgaagtgccc ggtggagtcg atgtagcacg
     5161 actcggcgat gctcatctcg ccgatcgcgg tgagcgacgc ctccgactgg tcggcgctca
     5221 cgtcggcgct cgtcggcagg tactcgaacg cgatccgctg gaggtacttg cagttgttcc
     5281 ggtaggggac gagcacccgc gcgagccggt cgggcaacat ctcccagcgt tcgggcgttg
     5341 ccgtcatgcg gcgatcccgt cgtagaacgg cacctggtcg tcgatgccga gccggaagat
     5401 ggtctggaag tcgtcgggcg ccatgtagcc cgccttgtgg atcagcgtgc ggttgtccca
     5461 gatgatgatg tcgcccagct cccacgagtg cggctgcacg tgctccggcc gctcgatgaa
     5521 cgcgaacagc tccacgagcg ccgcctggct ctcctcgtag ggtatcccgt cgatcgacgt
     5581 ggtgaagccc gagttcatgt agaggctgcg ccgccgggtc accgggtgcg tgatgacgag
     5641 cgggtgggtc gggggcgggt acaaccggcg acccatctcg aggagatcgc cgatgtcgcg
     5701 gttggcctcg acgtcggcgt ggctgacctt gtagcgcagc gccggattgt gcttggccgt
     5761 ccgtccgtcg acgatcgatc gcagcgcggc cggcagcgcg tcgtagatgg tcgccatgtt
     5821 gatgaacagc gtctcgcgct tcgcctgggg caggagctgc ggatagagca cggtcagcgg
     5881 ctgcggcctg ggcttgaacg agaggtcggt gtgccagaag tagccgctgc gcgccatgcc
     5941 catcgtccgg tccttgacgt tggacgacac gaacacgccg ggatgctcgg gatggcggta
     6001 ccgctcgtcg gggtatggca tgatcgtccc gagcttctcc ccgagggcca cgtactggtg
     6061 cacggtcatc ttctggttct tgaaccggac gagcgcgtgg tgatggacgg cggccaggac
     6121 gtcgcgcgct tgctcgctcg tgatcccgcc gaggtcgatc ccgtggatct gggcaccgag
     6181 gacaccgctg gctccgggaa tgatctgcat ggcactggct ccttgctgtg gtcggtggtc
     6241 tgtcacgatg cctggagggc ggcgatctgg tcgcccacgt cgcgcacgtc ccgcagctcg
     6301 agcccgagga gggactcgaa cacgcgacag gctcgccggt acgcgttgtc gactgcgctc
     6361 gccatggcct ggtggggcac ggcttcctgg atctccggga tcgaccggta gcgattcgcg
     6421 agcatgttga ccggatgccc gacgcgggcg ttgagctccg gcgtggtcca gaagtagagg
     6481 accttgagcg ccgcgagctg ctcgctcttc gggtcgtccg tgcggccgag ccgccgcttc
     6541 gcgtagctgt agaagctggc ggcgtggcga gcctcgtcac cgccgatgtt ccggtagatc
     6601 tgcttgagca cgggctcgca cgtcggctgg gcgctgatcg tcaagtagac cgccgcggcc
     6661 accatctccg agatcacgtt ggtcgcgagc gtgcccatct tcgacggcat gaacgggatg
     6721 gtctcccggc cctgcagcat gaagcggctg tcgaacttct cgccgagcgc gtcgagccac
     6781 ttgaggaccg ccgacgggtg cttggtctcc tcgtagagcc agaccgtcaa ccactgcgtg
     6841 aagtcgacgt catcgccgaa gtcggcgagg aaccgctcgg tggcgctgaa cgtggtgagc
     6901 tcgccgacca gggtctcgcg cgcgacccgg cgccaggccg gcgagacgtc ggccggccgg
     6961 agctccggcc acccgcagtc cgccatcttc caccggaccg cctcggcctc gtcgaacagc
     7021 cggtagaaca gcccgccgtc gtggatgttg gtctcgaacg ccgtcggttc gatgttcatc
     7081 gcagcacctc ctggaccgat gcggtccgct tccggatcgc gagcatgggg cagttgaaca
     7141 gcgcggcctc ggtcgccgcg atctccgccg cggtcgccgg ctggcgcacg atgaccgcgg
     7201 aggtctcgcc catcgcgatg atgccgggcg cgaggctgga gcaggcgccg cagcgcaggc
     7261 agtcgcccgc gatcgagtac gtggtgctca tgatgcactc cagagggtga ggaggtactg
     7321 gacgagcgac cggcaggtcg ggtggtcgaa cgccagcgtg gcgggcagct tggtcgacaa
     7381 ccggcgctcc aggcggaagc ggatgtccat cgccatcagc gagtccgcgc cgagctcctc
     7441 gaacggccga tcctcgtgga gggcgcccgg gtccggcagc ttgaggacgg ccgccacctc
     7501 ggtgcgcacc aggccgagca agaggctcgc acgctccgcc ggtggcgccg cgtcgaggcg
     7561 gtcgcgcagc gacggcgcag cggcaggggc aggcgcaggt ctggccgcgg cggcgggcgc
     7621 agcgaccg
//
LOCUS       DASVOR010002201         2007 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_199124,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002201 DASVOR010000000
VERSION     DASVOR010002201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199124"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VF516_40835"
     CDS             complement(<1..484)
                     /locus_tag="VF516_40835"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8114166.1"
                     /translation="MEDPINLLLVDDEARNLDALEAILDDPDYRLLRADSADAALRQL
                     LQNQIAAILLDIKMPGVSGFELANLIKGMKKYRQIPILFLTAYHLEDEDVLAGYSAGA
                     VDYLTKPVKPQILRHKVAVFADLVRKTRALAELTDTLELRVKQRTEELESSEAALRDA
                     N"
     gene            complement(508..>2007)
                     /locus_tag="VF516_40840"
     CDS             complement(508..>2007)
                     /locus_tag="VF516_40840"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8114167.1"
                     /translation="IAPEKQKLIFEAFQQADAGTSRKYGGTGLGLAISRELASLLGGE
                     IKLTSKPGSGSTFTLYLPIAYTGPAQRLVAPSGGQSVQTVMAMPVLTVAKTEETVPDD
                     RETIQPGDTVLLICEDDPHYARILLGIARGRGFKGLVANRGQQALALAREFMPTAITL
                     DVFLPDMLGWTVLNSLKLDSRTRHIPVQMLSVEEERQHGLSHGAFSYLVKPATTGELE
                     TALDRLKTYVAPHTKRLLVIEDNDVERESILELLGADDIEIVPAATGAEALAALHREP
                     IDCCVVDLRLPDMSGFELLERLQADEALRNIPVVVFTGKQLSGDEEARLRTVAKSIVL
                     KDVQSPERLLDETSLFLHRVIEDLPDHKRRMIERLHGSTEALRGRKVLVVDDDARNIF
                     ALATVLENQEMEVISAMNGRQAIELIQTTPDLSVVLMDIMMPEMDGYETMREIRKDFR
                     FRTLPILALTAKAMKGDREKCLQAGASDYIAKPVNTDQLLSLLRVWLYR"
BASE COUNT          284 a          664 c          696 g          363 t
ORIGIN      
        1 ggttggcgtc gcgcagcgcg gcctccgagc tctccagctc ctcggtgcgc tgcttgaccc
       61 gcagctccag cgtgtcggtg agctcggcca gcgcgcgggt cttgcggacc agatcggcga
      121 acaccgcgac cttgtggcgc aggatctggg gcttgaccgg cttggtcagg tagtcgaccg
      181 cgcccgcgct gtagccggcc aggacgtcct cgtcctcgag gtggtacgcg gtgaggaaca
      241 ggatcgggat ctggcggtac ttcttcatgc ccttgatcag gttcgccagc tcgaagccgc
      301 tgaccccggg catcttgatg tcgagcaaga tcgccgcgat ctggttctgc agcagctggc
      361 gcagcgcggc gtcggcgctg tctgcgcgca gcaggcggta gtccggatca tcgaggatcg
      421 cttccagcgc gtcgaggtta cgcgcctcgt cgtccacgag taacagattg atggggtctt
      481 ccatccgagg taccgatctt actgatctca gcgatacaac cacacccgga gcagcgacaa
      541 caactgatcc gtgttgacgg gttttgcgat gtagtcggag gcgccggcct gcaggcactt
      601 ctcccggtcg cccttcatcg ccttggcggt cagcgcgagg atcggaagcg tgcggaaccg
      661 gaaatccttg cggatctcgc gcatggtctc gtagccgtcc atctcgggca tcatgatgtc
      721 catcaggacc acactgaggt ccggtgtggt ctggatcagc tcgatcgcct ggcggccgtt
      781 catcgcgctg atcacctcca tctcctggtt ctccagcacg gtcgcgagcg cgaagatgtt
      841 gcgggcgtcg tcgtcgacca ccagcacctt gcggccgcgc agcgcctcgg tcgatccgtg
      901 caggcgctcg atcatcctgc gcttgtggtc cggcaggtcc tcgatgacgc ggtgcagaaa
      961 cagcgaggtc tcgtcgagca gccgctcggg cgactggacg tccttgagca cgatgctctt
     1021 ggcgaccgtg cgcagccgcg cctcctcgtc gccgctgagc tgcttgccgg tgaacaccac
     1081 gaccgggatg ttgcgcagcg cctcgtcggc ctgcagccgc tccagcagct cgaagccgga
     1141 catgtcgggc agccggaggt cgaccacgca gcagtcgatc ggctcgcggt ggagcgcggc
     1201 cagcgcctcg gcgccggtcg cggccgggac gatctcgatg tcgtccgcac cgagcagctc
     1261 gaggatgctc tcgcgctcga cgtcgttgtc ctcgatcacg agcagccgct tggtgtgggg
     1321 cgcgacgtag gtcttgagcc ggtcgagcgc ggtctcgagc tcgccggtgg tcgccggctt
     1381 gaccaggtac gagaacgcgc cgtgcgacag cccgtgctgg cgctcctcct cgaccgacag
     1441 catctggacc gggatgtggc gggtccgcga atcgagcttg aggctgttga gcacggtcca
     1501 gcccagcatg tcgggcagga acacgtcgag cgtgatcgcg gtcggcatga actcgcgggc
     1561 gagcgcgagc gcctgctggc cgcggttggc gaccaggccc ttgaacccgc ggccgcgcgc
     1621 gatcccgagc aggatccggg cgtagtgcgg atcgtcctcg cagatcagca gcaccgtgtc
     1681 gccgggctgg atggtctcgc ggtcgtccgg gacggtctcc tcggtcttgg cgaccgtcag
     1741 gaccggcatc gccatcacgg tctggaccga ctgcccgccg ctcggcgcga ccaggcgctg
     1801 ggccgggccg gtgtacgcga tcggcaggta cagcgtgaac gtgctgccgc tgcccggctt
     1861 gctggtcagc ttgatctcgc cgccgagcag gctggcgagc tcgcggctga tcgccaggcc
     1921 caggccggtg ccgccgtact tgcggctggt gccggcgtcg gcctgctgga acgcctcgaa
     1981 gatcagcttc tgcttctcgg gcgcgat
//
LOCUS       DASVOR010002202        12173 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_140509,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002202 DASVOR010000000
VERSION     DASVOR010002202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 12173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12173
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140509"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5216)
                     /locus_tag="VF516_40845"
     CDS             complement(<1..5216)
                     /locus_tag="VF516_40845"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HEX8114168.1"
                     /translation="MELPRLLGVMVVCWVGVAAADPASSGLTWILSQQAADGSFGGTT
                     GEQKVLATVEALETLRAAGQGQTPAAHAAELFLAIAPRTQDAEVELRRELALAPTGQA
                     LSLRVRTSHATGFEHKDALHLALTLRNDIAAGRAGDAESFQRMLDLEARQLASGCWRW
                     ADNDDSVELTSDAVRTLRALLDVPTEGRASNGHLDAALDEGARCLGNASGGSLSATAM
                     RLEALLAAPGTHTTQIADARAVLAAAQASDGSIGGTVRTTAIAVRGLLPGKPDWRIVP
                     DDFNRPVLAFDDANPLAGIDAVARLNIDNRSGTAAPATTVRILLRPLDGGAEQVGAEA
                     PLGALAAGASAALAVHVPSAALQGKYSVRAVVDPDHVVAEISEANNEARQLLNVRAEA
                     DLSVAGSSIRFTPVAAGQTRVEVTVRNLGIPLDRPVSVGVWKGNPSTGGVRIGGATLA
                     AGLPTNGTGTVSVMWSSSSANGPTVVTAVVDELDVLPEADETNNAAFRFYFPGSDQPV
                     DLAVADADVAFTPGQLHVGDLVTFTAVVKNLSSFDASRVPVAMTDTGGVIVGQTELPF
                     VPAHGSAVASFRFAMTNTSVSGRIQVDPAQQLNDSDRSNNTAAGPMARASATPDVDFN
                     VLSAVDRSSTRTVEVLVRNNGTKPVATTVELFDITHGISLGSQPIVLPTGYATSFSES
                     LSFSIPADTSPAVLRACADPSNVVAEPNENDNCAEVALSGFERVDLITRSKDLRFSPV
                     GAAVGENVHVTATVHNQGGFAGRSRPAKGVVEFWHSVPGSKAGKFLGTVPFAVNASSD
                     VTVDFDWVRADGPVELCVRVAQVQPADQVALNNIACRNLFLEQVVDLFPATMFQSGEN
                     LRVARLTGRPAPEILVAYEDTLPSGPLTGETGLMVLQRSAGGTYAPLWSVPMPADTRP
                     TDIVVADLDGDGAAEVVLFTAFQDHGGTLPSTSGVTAYRADGSVKWTTSFVPKMARPG
                     LSQRGNHFGEATGGQLSVGDVNGDGVADIVTVEETVRVFSGDDGHLVWSAPNPAVFFS
                     SPPTDCRATVLDVDGGGTHEVFSSCGAMFLLSSTGSLVWTNPSNCSFHHQVIDLDLDG
                     SPEGVVACEGNLVALDLRNGAQKARLSAAWIWGNATVPFGAVLQDGLPYAVASDNSGS
                     TTIFGFRPSMDVLWQQSVARPDNGDVYAGLLVDLLGLGRPQFVASSPTRPLLLQDGRD
                     GRLLLEVPLSQFGAIAGSNFTYGWLAPVVADLGTGHASVLVTRSGARGEIGSGGPPHD
                     GAGEFLIMSSPHWTKQPALWATRVMRHGQIDETTLKVSNDYRWWTSHNTWQQQYDVAP
                     AKLLPDLTVALAASPAPGTAGEATTLTATVKNLGGAPATTAVVSFFDGDPSNGGKAIG
                     TAPVGGSVAGRGGTSTATLSWTAFPEGEHMLFAVAGGVEESGTENNSARLRYFVQPGA
                     QLCDAAVNGSTLAASPAQPSAGQAVQLSATVTNLGPIACAAHVLTVRDGAGGEVLGTV
                     SVPSLAQGAQATVAVSFRAIPGSHLLRVIADEAQTLQDGDLANNVATLALDVPPAIQP
                     DYTATSIVLTPGPARAGELVTARVTVKNQGVAVTAQTRLEVSVDAQTVGSVTVDALGA
                     SESKTYTVKLVAPSAATAVVARVDADGALTEFDETNNVRSAPLAIESSGLVLTGSATP
                     SSALPDTSVRFTVLAQLSSPSAKEVWFDATLLGAGGVSVATVASGVREVIVSGSSSRS
                     FTLDTGRLAPDT"
     gene            5401..5619
                     /locus_tag="VF516_40850"
     CDS             5401..5619
                     /locus_tag="VF516_40850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114169.1"
                     /translation="MQRRSRPRPTEIRRDTETEAEELGQVAGYLDRAGLGEGWLVMFD
                     LRKDLPWVDKLFTREVEHAAKRVRLVGC"
     gene            5935..8469
                     /locus_tag="VF516_40855"
     CDS             5935..8469
                     /locus_tag="VF516_40855"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX8114170.1"
                     /translation="MRAWAVCLGVVIQLGAALPAHALDGHRRVTQYAQTHFGARDGMP
                     HGYSTAIIQTTDGYLWNASQEGLSRFDGAGFTNYDARKTEGVPVNVFTALAVDSAGTL
                     WTGTFDHGVLHLVDGTFRTVAWEPGPQEQMVRALAFDGNDDLWIGMRDRGVVRLHAGK
                     FDAALTTHDGLPSDDIRTFLRGRDGAMWIGTFKGLARWKAGQITRGPALLEGTAIYAL
                     AEDAEGDLWCATENGLAHVHGDTAELLDAGHLPARDVHAVRFDRDHNLWIGTSAGVVR
                     MTPDGEIQRLPHPDGGINALFEDREGNLWIGGDKGLDRLFDGDMLPVGSAEGLTDEGA
                     RAVREDASGTLWITTNVGLFVIPPGQTLATKIAAADRGVMYAIYQDSHGDMWFGGRGD
                     VGRWHAGRFTWLGHERWERIRAFVEMPDGMWVGTNHGAFQLEVQGDRLVGVHPVLQDI
                     SINAMVPDRAGSLWLGTAGAGLMRWRSGAPAPIPPGGPPRNYPAATILFDPDGTMWVG
                     TIGAGLWRLRDGRWAAFTSKDGMFDDLVWSIIDDGLGNFWMSSDRGIWRVSRQQLDAR
                     AAGLRATVDSVVYGEADGMRERECNGAQDPAGWRTRDGRLWFPTAMGLAMIDPAHLHA
                     SKPPGTLIASVRVDGELQPTGRDLVLRPGASRLEIGYTAPALRGPVRLRFRYRLDGFD
                     HQWNEAGAQRVAQYTNLPPGEYHFVVEAGLDDAWGASSTIAMSRPPLFYETGWFYALA
                     TLSLALAIVAVPLLRVRQLRTRARDLDQRVQEAIGELKVLSGLLPICAWCKKIRDDRG
                     YWSKIEAYLSARTEAQFTHGICPDCTEKMLSEESFGRSSISQGSRR"
     gene            8601..11120
                     /locus_tag="VF516_40860"
     CDS             8601..11120
                     /locus_tag="VF516_40860"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX8114171.1"
                     /translation="MSFAIVIQLGAVGDAFALDSHRRVTQYAHAHFAAHEGMPHSLAS
                     AIAQTADGYLWTSSQEGLSRFDGASFTTYDHRQTEGIPVNQFKALAVDREGTLWAGTA
                     NRGVIHLVGAEFRPVAWEPGPQEQPVSVLEFDPAGDLWIGTRDRGVIRLHDGKFAAAL
                     TTHDGLPSDDIRALLATSDGAMWIGTFRGLVRWTSGGLVRGPAALDGVAIHAIAQAAG
                     VVWCATDKGLVRLRGDSIEPIGADRLSPGEVRKLLFDRDANLWLGTRMGVARMTFDGQ
                     GGVEIERLPEPQILINALFEDADRNLWIASDRGLDRLSDGDVLPMGASEGLVDEPVGA
                     IREDATGAMWITTIAGLYRIAPGQTTATRINTDRGLYAIYPDSRGDVWFGGRDGNIGR
                     WHDGRLVLLGNRDWERVRSLTETADGLWLGTEHGLFRMRGDRLDDAVGVVPGVSVHAI
                     VADKLGALWIATENGGLVRWQNGAPSAIPRGGPPRNTAVTAITFDADGTMWVGTEGAG
                     LWRLRDNRWFVFTTHDGMFDDLVWRILDDGLGNLWMSSNRGIWRVNRQQLEAVAAGQR
                     ATVDSVVYGETDGMRDRECNGVADPPGWRTRDGRLWFPTGKGLVVIDPAHLHERQPPH
                     ALIESVRVNGRPQPTTGPLVLPAGSPRLELGYTAPALRGPDRLRFRYRLVGFESDWNE
                     VGAQRVAQYTNLPAGDYRFTVEARIDDKWGKASSIAMTLPPLFYEAGWFYALATLSLA
                     LAIVAVPLLRVRQLRRRARDLDQRVQEAIGELKVLSGLLPICAWCKKIRDDRGYWSKI
                     EQYLSARTEAQFTHGICPDCTEKMLSDESFGRSSISQGGRR"
     gene            11921..>12173
                     /locus_tag="VF516_40865"
     CDS             11921..>12173
                     /locus_tag="VF516_40865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114172.1"
                     /translation="MSTLDRKRGDQSLVQRATADAASDHGPGKRTLTEQLGPGVPGAA
                     PEHTAAAHQVAERDALNAVITVVARGPDGAVVARWRARGR"
BASE COUNT         1857 a         4239 c         4262 g         1815 t
ORIGIN      
        1 taggtgtcgg gcgcgaggcg tccggtgtcg agcgtgaacg agcggctgct cgatccggaa
       61 acgatgacct cgcgcacccc ggaggccacc gtggcaaccg acaccccgcc ggcgccgagg
      121 agcgtcgcgt cgaaccacac ctccttcgcg gacggcgagg acagctgcgc cagcaccgtg
      181 aagcggaccg acgtgtcggg tagcgccgag gacggcgtcg ccgagcccgt caacaccagg
      241 cccgagctct cgatcgcgag cggtgcggag cggacgttgt tggtctcgtc gaactccgtc
      301 agcgcgccat ccgcgtcgac ccgtgcgacc accgcggtcg ccgccgacgg tgcgacgagc
      361 ttcacggtgt acgtcttgct ctcgctggca cccagcgcgt cgaccgtcac cgaaccgacc
      421 gtctgtgcgt ccaccgagac ctcgaggcgg gtctgcgcgg tgaccgcgac accctggttc
      481 ttcaccgtca cgcgggcggt gacgagttct ccggcgcgcg cggggccggg ggtcagcacg
      541 atcgacgtcg cggtgtagtc gggctgtatg gcggggggca cgtcgagcgc cagcgtcgcc
      601 acgttgttgg ccaggtcgcc gtcctgaagg gtctgcgcct cgtcggcgat gacacggagc
      661 aggtggctcc cgggaattgc acggaacgac accgccacgg tggcctgggc gccttgagca
      721 agcgatggca ccgagaccgt tccgagcacc tcgccgcccg cgccgtcgcg gaccgtcaac
      781 acgtgggccg cgcatgcgat cgggccgagg ttcgtgacgg tcgctgacag ctgcaccgcc
      841 tggccggccg acggttgcgc gggcgaggcc gccagcgtcg agccgttgac cgccgcatcg
      901 cacagctgcg cccctggctg cacgaaatag cgcagtcgag ccgagttgtt ctcggtaccc
      961 gattcctcga cgccgccggc gacggcaaac agcatgtgct cgccctcggg gaaggccgtc
     1021 caggacagcg tggccgtcga tgtgccgccg cgaccggcga cgcttccgcc caccggtgcg
     1081 gtgccaatcg ctttgcctcc gttcgatggg tcgccatcga agaacgacac cacggccgtc
     1141 gtcgcaggcg ctccgccgag gttcttcacc gtcgcggtca gcgtggtcgc ttcgcccgcc
     1201 gtgcccggcg ccgggctcgc cgcgagtgcc accgtcaagt ccgggagcag cttcgccggc
     1261 gcgacgtcgt actgctgctg ccaggtattg tgcgacgtcc accagcggta gtcgttcgac
     1321 accttcagcg tcgtctcgtc gatttgcccg tgtctcatca cccgcgtcgc ccacagcgcc
     1381 ggctgcttgg tccagtgcgg actggacatg atgagaaact ctccggcgcc gtcatgcggt
     1441 gggccgccgg agccgatctc gccgcgggca ccgctgcgcg tcacgagcac gctcgcatgg
     1501 ccagtgccga gatccgcgac caccggagcc aaccagccat acgtgaagtt ggagccagcg
     1561 atggcaccga actgactcag cggcacctcc agcagcaggc gcccatcccg cccatcctgc
     1621 agcagcagcg gccgagtcgg tgagctggcg acgaattgcg gccgcccgag gccgagcagg
     1681 tcgaccagga gcccggcata gacatcgccg ttgtccgggc gcgcgaccga ctgctgccag
     1741 aggacatcca tgctcggacg gaagccaaag atggttgtgc tgcccgagtt gtcggacgcg
     1801 acggcgtagg gcaggccatc ttgcagcacc gcgccaaacg gcacggtcgc attcccccag
     1861 atccacgccg ccgacaagcg cgctttctgc gctccattgc gaaggtccag tgccaccagg
     1921 ttcccttcgc aggcgacgac gccttccgga ctgccatcaa gatccaggtc gatcacctga
     1981 tggtggaagc tacagttgct gggattggtc cacaccagcg agccggtgct cgacaagagg
     2041 aacatcgccc cgcagctact gaacacctcg tgggtgccgc cgccgtcgac gtcgagcacg
     2101 gtggcgcggc aatccgtggg gggcgaggag aagaagaccg cggggttggg ggcgctccac
     2161 accaggtgac catcgtcgcc cgagaacacc cggaccgtct cctcgacggt gacgatgtcc
     2221 gccacgccat ctccgttcac atcgccgacg ctgagctgcc cgcctgtggc ttctccgaag
     2281 tggtttccgc gctgggacag cccgggccgg gccatcttgg ggacgaagct cgtcgtccat
     2341 ttgacggagc catccgcgcg gtacgccgtc acgccgctcg tggatggcag cgtcccgccg
     2401 tggtcctgga acgccgtgaa caggacgacc tcggcggcgc cgtctccgtc gaggtccgcc
     2461 accacgatgt cggtcgggcg cgtgtcagcc ggcatcggga cgctccacag cggcgcatac
     2521 gtcccccctg cgctccgctg gagcaccatc aggcctgtct ccccggtcaa tgggcccgag
     2581 ggaagcgtgt cttcgtaggc gacgaggatc tcgggcgccg ggcggcccgt cagccgcgcg
     2641 acgcggagat tctctccgga ctggaacatc gtcgcgggaa acaggtccac cacctgctcg
     2701 aggaacaggt tccggcaggc gatgttgttc aaggccacct gatccgcggg ctgcacctgc
     2761 gccacccgca cgcacagctc gaccggaccg tccgcacgca cccagtcgaa gtctacggtg
     2821 acgtcggacg acgcattcac cgcgaacggg acggtgccga ggaattttcc tgccttcgag
     2881 ccaggcacgg aatgccagaa ctccaccacg cccttcgcag gtctcgagcg cccggcaaag
     2941 ccgccctggt tgtgcactgt cgccgtcaca tgcacgttct ctcctaccgc cgcgcccacc
     3001 ggactgaagc gcaggtcctt cgagcgggtg atgaggtcga cgcgctcgaa gccactgagt
     3061 gccacctccg cgcagttgtc attctcgttt ggctcggcga cgacgttgct ggggtccgcg
     3121 caggctcgca ggaccgcggg gctggtgtcg gccggaatcg aaaatgagag agactctgag
     3181 aaactggtcg cgtaaccggt cggcaggacg atcggctggc tgcccagcga gatgccgtgg
     3241 gtgatgtcga acagctcgac ggtggtcgcg accggtttcg tgccgttgtt cctgaccaac
     3301 acctccacgg tgcgggtgga ggagcggtcc acggccgaga gcacgttgaa gtccacgtcg
     3361 ggcgtcgcgc tcgcccgcgc catgggtcca gctgcggtgt tgttgctgcg atcgctgtcg
     3421 ttgagttgct gcgcggggtc gacctggatg cgcccggaga cgctcgtgtt ggtcatcgcg
     3481 aaccggaagc tggccaccgc tgaaccatgg gcggggacaa acggcaactc ggtctgcccc
     3541 acgatgacgc ccccggtgtc cgtcatcgcc accggcaccc ggctggcgtc gaagctggac
     3601 aggttcttca ccaccgcggt gaacgtcacc aggtcgccca cgtgcagctg accgggggtg
     3661 aaggcgacgt cggcatcggc cacggccagg tcgacgggct ggtcgcttcc cgggaagtag
     3721 aagcggaacg ccgcgttgtt ggtctcgtct gcctcgggga ggacgtcgag ctcgtccacg
     3781 actgcggtca ccaccgtggg accgttcgcc gatgacgagc tccacatcac cgacaccgtg
     3841 ccggtcccgt tggtggggag gccggcggcg agcgtcgctc cgccgatgcg cacgccgccg
     3901 gtcgacgggt tgcccttcca caccccgacg gacaccggcc ggtccagcgg aatgccgagg
     3961 ttgcgcaccg tcacctcgac gcgggtctgc ccggctgcga ccggcgtgaa ccggatgctc
     4021 gagccggcca ccgagaggtc cgcttcggcg cggacgttca gcagctgtcg cgcctcgttg
     4081 ttggcctccg agatctcggc cacgacgtga tcggggtcca ccacggcccg gacggagtac
     4141 ttgccctgca gcgccgcgct gggaacgtgg accgccagcg ccgcgctggc tcccgcggcc
     4201 agcgcgccca gcggcgcctc ggcgccgacc tgctccgcgc cgccgtcgag cgggcgaagc
     4261 aggatgcgca ccgtcgtcgc aggagctgcc gtccccgagc ggttgtcaat gttcagccgt
     4321 gccacagcgt caatgcccgc cagtgggttg gcatcgtcga acgccagcac cgggcggttg
     4381 aagtcgtccg ggacgatgcg ccagtcgggt ttccccggca gcaggcctcg caccgcgatc
     4441 gccgtcgtcc tcaccgtgcc gccgatgctc ccgtcgctcg cctgggccgc ggccagcacc
     4501 gcgcgcgcgt ccgcgatctg cgtggtgtgg gtccccggcg ccgccagcag cgcctccagc
     4561 ctcatcgccg tcgcgctcaa cgagccaccg ctcgcgttgc ccaggcagcg cgcgccctcg
     4621 tcgagcgcgg cgtcgaggtg gccattcgag gcccgtcctt ccgtcggtac atcgagcaac
     4681 gcgcgcaggg tgcgaacggc gtccgaggtg agctccaccg agtcgtcgtt gtcggcccac
     4741 cgccagcaac cggaagccag ctggcgcgcc tcgagatcga gcatgcgctg gaagctctcg
     4801 gcgtcgcccg ctcgccctgc ggcgatgtcg ttccgcagcg taagcgccag gtgcagtgcg
     4861 tccttgtgct cgaatccggt cgcgtggctg gtccggacgc ggagcgacaa cgcttgtccc
     4921 gtcggagcga gcgcaagctc ccggcgcagc tcgacctcgg catcctgcgt ccgaggagcg
     4981 atcgccagga acagctcggc ggcatgcgcg gccggcgtct gaccctgtcc ggcggcgcgc
     5041 agcgtctcga gtgcctcgac ggtcgcgagc accttctgct cgccggtcgt tccgccgaag
     5101 ctgccatcag cggcctgttg cgagagaatc caggtgagcc ccgacgacgc cggatccgcc
     5161 gctgcgaccc ccacccagca cacgaccatg accccaagca gccttggcag ctccatcgac
     5221 gccccccgaa tccttcacgc gtcgaatccc ccgacgctac aaatcactct accgcatttc
     5281 tgtctcccac caagctggtc atcaactcca tgctgaagat cttcgatcgt gcgctccgca
     5341 tgcgacacgc ggagctgtct cgacccagtg cagagccagt gcagagccag tgcagagcca
     5401 gtgcagagga ggtcgaggcc acgccccacg gagatccgcc gcgataccga gaccgaggcc
     5461 gaggagctgg gacaggtcgc gggctacctg gatcgcgcgg ggctcggcga gggctggctc
     5521 gtgatgttcg acctgcgcaa ggatctgccc tgggtggaca agctgttcac ccgcgaggtg
     5581 gagcacgccg ccaagagagt tcgcctcgtg ggctgctgac ggccccggcc cggccccggc
     5641 caacctcgcc ccccgcgcac ggcgagtgac atgtcgcaag gtcagagtgc gccatgtcgc
     5701 cgcgagatgt gtcaaacgca gtcagccgtg tgctgcatct gcttgcgcca ccatcagaaa
     5761 tccaggctat gactcacccg gtcatgcgat gaagtgacgt cgaggtcgga ccacatcgcc
     5821 tggctggcgt atgcgctacc gcacatgcgg cccgatgcgg ggccacatgg ccgaacaaca
     5881 gggtagaacc ggcgggcggc ggccgggtac gctggtcgct ccggggaaat tctgatgcga
     5941 gcgtgggccg tttgtcttgg tgtcgtcatt caactgggcg cagcgctgcc cgcgcacgcg
     6001 ctcgatggtc accgccgcgt cacgcagtac gcacagaccc acttcggggc tcgcgatggc
     6061 atgccgcacg gctactccac cgccatcatc cagaccaccg atggctacct gtggaacgcc
     6121 agccaggaag ggctgtcgcg cttcgacggg gccgggttca cgaactacga tgctcggaaa
     6181 accgaggggg ttcccgtcaa cgtcttcacg gcgctggcgg tcgactccgc cgggacgctg
     6241 tggaccggca cctttgatca cggagtgctg catctggtcg acgggacgtt tcgcacggtc
     6301 gcatgggagc cggggccgca ggagcagatg gtccgggcgc tcgcgttcga tggcaacgac
     6361 gacctgtgga tcggcatgcg cgatcgcggc gtggtccggc tgcacgccgg caagttcgac
     6421 gcggcgttga ccacgcacga cgggctgccc agcgacgaca tccgcacctt cctgcgcggg
     6481 cgcgacggcg cgatgtggat cggcacgttc aagggactcg cgcgctggaa ggccggccag
     6541 atcacgcgcg ggcctgccct gctcgagggc accgcgatct atgcgctcgc cgaggacgcg
     6601 gagggcgatc tgtggtgcgc caccgagaac gggctcgctc atgtccacgg cgacaccgcc
     6661 gagctgctcg acgccggtca cctgccggcc cgcgacgtcc acgccgtgcg gttcgatcgc
     6721 gaccacaacc tgtggatcgg caccagcgcc ggggtcgtgc gcatgacgcc ggacggagag
     6781 atccagcggc tgccccaccc cgacggaggc atcaacgcgc tgttcgagga ccgcgagggc
     6841 aacctgtgga tcggcggcga caaggggctc gatcggctgt tcgacggcga catgctcccg
     6901 gtcggaagcg ccgaggggct gaccgacgag ggcgcgcgcg ccgtgcgcga ggacgccagc
     6961 ggcacgctgt ggatcacgac caacgtgggc ttgttcgtga tcccacccgg ccagaccctg
     7021 gcgaccaaga tcgccgccgc cgaccgcggc gtgatgtacg cgatctatca ggactcgcac
     7081 ggcgacatgt ggttcggcgg acgcggcgac gtcggccgct ggcacgccgg ccggttcacg
     7141 tggctcgggc acgagcgctg ggagcgcatc cgggcgttcg tcgagatgcc cgatggcatg
     7201 tgggtcggga ccaaccacgg cgcgttccag ctcgaggtgc agggcgatcg cctggtcggt
     7261 gtccacccgg tcttgcagga catctcgatc aacgcgatgg tccccgaccg cgcaggctcg
     7321 ctgtggctgg ggacggcggg ggcgggcctg atgcgctggc gctccggtgc gccggcgccg
     7381 atccctccgg gcggaccgcc gcgcaactac ccggccgcca cgatcctgtt cgatcccgat
     7441 ggcacgatgt gggtagggac gatcggggcc gggctgtggc ggctgcgcga cggccgctgg
     7501 gccgcgttca cctccaagga cgggatgttc gacgatctgg tgtggagcat tatcgatgac
     7561 gggctcggca acttctggat gtcgtcggat cgcgggatct ggcgggtgag ccggcagcag
     7621 ctcgacgcgc gcgcggccgg gctgcgcgcg accgtcgact cggtggtcta cggcgaggcc
     7681 gacggcatgc gcgagcgcga gtgcaacggc gcgcaggatc cggcgggctg gcggacccgc
     7741 gacggccggc tgtggttccc gaccgccatg ggcctggcca tgatcgatcc ggcccacctg
     7801 cacgccagca agccgccggg tacgctgatc gcgagcgtgc gcgtcgacgg cgagctgcag
     7861 cccaccggcc gcgatctggt gctgcggccc ggcgccagcc gcctcgagat cggctacacc
     7921 gcgccggcgc tgcgcggccc ggtccggctg cggttccgct accggctcga cggcttcgac
     7981 caccagtgga acgaggccgg cgcgcagcgg gtcgcgcagt acaccaacct gccgccgggc
     8041 gagtaccact tcgtcgtcga ggccgggctc gacgacgcgt ggggcgcgtc cagcacgatc
     8101 gcgatgtcgc ggccgccgct gttctacgag accggctggt tctacgcgct cgccacgctg
     8161 tcgctcgcgc tggcgatcgt cgcggtgccc ctgctccggg ttcgccagct ccggacgcgc
     8221 gcccgcgatc tcgaccagcg ggtccaggag gcgatcggcg agctcaaggt gctgtccggc
     8281 ctgttgccga tctgcgcgtg gtgcaagaag atccgcgacg atcgcggcta ctggagcaag
     8341 atcgaggcgt acctcagcgc gcgcaccgaa gcccagttca cccacgggat ctgccccgac
     8401 tgcaccgaaa agatgctttc cgaggagagc ttcggtcgca gcagcatcag ccagggatcg
     8461 cggcgatagc cgcggtcacg accggccggc cggcgtccgt gccacatggg tggccaacgg
     8521 actacatcgc ggatggtcgc tcacgacccg gggccggggt aggctagtcg ttctgagcca
     8581 tcgtgatgcg cgcgtgggcc gtgagcttcg ctatcgtgat ccagctgggg gcagtggggg
     8641 atgcgtttgc cctcgactcc caccgccggg tcacgcagta cgcgcacgcc cactttgcgg
     8701 cccatgaggg aatgccgcac agcctggcca gcgcgatcgc gcagaccgcg gatggctacc
     8761 tgtggacttc cagccaggag gggctgtcgc ggttcgacgg cgcgtcgttc accacctacg
     8821 accaccggca gaccgagggc atcccggtca atcagttcaa ggcgctcgcg gtcgatcgcg
     8881 aggggacgct gtgggcaggg accgccaacc gcggcgtgat ccacctggtc ggcgcggaat
     8941 ttcgccccgt ggcgtgggag ccgggcccac aggagcagcc ggtcagcgtg ctggagttcg
     9001 acccggccgg cgacctgtgg atcgggacgc gcgatcgcgg cgtgatccgg ctgcacgacg
     9061 gcaagttcgc cgcggcgctg accacgcacg acgggctgcc cagcgacgac atccgcgcgc
     9121 tgctcgcgac cagcgacggc gcgatgtgga tcggcacgtt ccgcgggctc gtgcggtgga
     9181 cgtccggcgg actggtgcgc ggcccggcgg cgctcgacgg cgtggcgatc cacgcgatcg
     9241 cgcaggcggc cggcgtcgtg tggtgcgcga ccgacaaggg gctcgtgcgg ctgcgcggcg
     9301 actcgatcga gccgatcggc gcggatcgcc tgtcgcccgg tgaggtccgc aagctgctgt
     9361 tcgaccgcga cgccaacctg tggctcggca cgcgcatggg ggtggcgcgg atgacgttcg
     9421 acggccaggg cggggtcgag atcgagcggc tgccggaacc ccagatcctg atcaacgcgc
     9481 tgttcgagga cgccgaccgc aacctgtgga tcgccagcga tcgcgggctg gatcggctga
     9541 gcgacggcga cgtgctgccg atgggcgcca gcgaggggct ggtcgatgaa ccggtcggcg
     9601 cgatccgcga ggacgcgacc ggcgcgatgt ggatcaccac gatcgccggg ctgtaccgga
     9661 tcgcgccggg gcagaccacg gcgacccgga tcaacaccga ccgcgggctg tacgcgatct
     9721 atcccgactc gcgcggcgac gtgtggttcg gcggccgcga cggcaacatc gggcgctggc
     9781 acgacggccg gctggtgttg ctgggcaacc gggactggga gcgcgtccgc tcgctgaccg
     9841 agaccgccga cggcctgtgg ctcggcaccg agcacggcct gttccgcatg cgcggcgacc
     9901 ggctggacga cgccgtcggg gtggtgcccg gggtctcggt ccacgcgatc gtcgccgaca
     9961 agctgggcgc gctgtggatc gcgaccgaga acggcggcct ggtgcgctgg cagaatggcg
    10021 cgccgtccgc gatcccgcgc ggcggaccgc cgcgcaacac ggcggtcacg gcgatcacgt
    10081 tcgacgccga cgggacgatg tgggtcggca ccgagggcgc cgggctgtgg cggctgcgcg
    10141 acaaccggtg gttcgtgttc accacccacg acgggatgtt cgatgacctg gtgtggcgga
    10201 tcctcgacga cgggctgggc aacctgtgga tgtcgtccaa ccgcgggatc tggcgggtca
    10261 accggcagca gctcgaggcg gtcgccgcgg ggcagcgcgc gaccgtcgac tcggtggtct
    10321 acggcgagac cgacggcatg cgcgaccgcg agtgcaacgg cgtggcggat ccgccgggct
    10381 ggcggacccg ggacggccgc ctgtggttcc cgaccggcaa gggcctggtc gtgatcgacc
    10441 ccgcgcacct ccacgagcgc cagccgccgc acgcgctgat cgagagcgtg cgggtcaacg
    10501 gccggccgca gccgaccacc gggccgctgg tgctccccgc cggcagcccg cggctggagc
    10561 tcggctacac cgcgccggcg ctgcgcggcc ccgaccggct gcggttccgc taccggctcg
    10621 tcggcttcga gtcggactgg aacgaggtcg gcgcgcagcg ggtcgcgcag tacaccaacc
    10681 tgccggccgg cgactaccgg ttcaccgtcg aggcccggat cgacgacaag tggggcaagg
    10741 cgtcgtcgat cgcgatgacg ctgccgccgc tgttctacga ggccggctgg ttctacgcgc
    10801 tcgccacgct gtcgctcgcg ctggcgatcg tcgcggtgcc cctgctccgg gtccgccagc
    10861 tccggcggcg cgcgcgcgat ctcgaccagc gggtccagga ggcgatcggc gagctcaagg
    10921 tgctgtccgg cctgttgccg atctgcgcgt ggtgcaagaa gatccgcgac gaccgcggct
    10981 actggagcaa gatcgagcag tacctcagcg cgcgcaccga ggcccagttc acccacggga
    11041 tctgtcccga ctgcaccgag aagatgctgt ccgacgagag cttcggccgc agcagcatca
    11101 gccagggcgg gcgtcgctag cgccgcgtgc atcaacgaca tcatcttgtc ggctgccggt
    11161 gcggcgccgc gaaggtaggt caacgatcgg cgaacgtgcg ggctggcggg gctccagcca
    11221 cattgccgca agttataaat gtcagttgac aaagtaaatt ctgtgacaca actatgtatt
    11281 tttgacaacc gggcaaggca acacgttcgt tcctgctgct cgtcttcgca agtcgtctgg
    11341 ccggcgcgaa actcggggct ccttcaggct ccttgagcag ctcgaggttc tccgcatcga
    11401 ggccgagggg gcatcaggga gccgggcaag cagctcggaa tgtcgccgcc cggccccgct
    11461 cgagatcgtc acgtctggct cgtcgccgca tccgcccgac gtccgccctt gccaagcgcg
    11521 ccgtgcggcg catccacacc agtttcaagc tctccaccaa ggatcggccg aagcgggtgc
    11581 cggcccaggt caaagagtgg caacttcagg ccagagataa accttgtccg gacctcggac
    11641 aatgtgggga ttgagcctct gagaaattag gttccacgtc aggcgagggc gccctcgcca
    11701 cagcatgagc gccctcgcca cagccagccg tgagtaaccg tgagaccgac gagcgcacgg
    11761 cacgcgccgt cggaaatact ggtgtcacct tcccaggctt ccagtcgact gttctgtccg
    11821 gcggcttggt cgtggtgcgg ttcgacgtga ctgaggcggg ctactacatc ggcaaggtaa
    11881 cggcgacctt cgagcgtccg tggcccggag gagccccgct gtgagcaccc tcgaccgcaa
    11941 gcggggcgac cagagcctcg tccagcgcgc caccgccgac gctgcgtccg accatgggcc
    12001 cggcaagcgg acgctcaccg agcaactcgg ccccggagtc cctggcgccg cgccagagca
    12061 caccgccgct gcccaccagg tggccgagcg cgacgccctc aacgcggtga tcacggtcgt
    12121 cgcccgcggc ccggatggcg ccgtcgtcgc ccgctggcga gctcgtggcc gct
//
LOCUS       DASVOR010002203         2347 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_199129,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002203 DASVOR010000000
VERSION     DASVOR010002203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199129"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            350..1747
                     /locus_tag="VF516_40870"
     CDS             350..1747
                     /locus_tag="VF516_40870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114173.1"
                     /translation="MRHALLAAIACSAVPGCTTTEPPLEPAVAAAVTGTVQLNLLYIH
                     GVENSASARTGAHNTLNDLKAAVAADLASLIASYQASHPGVTIHAASASANLYTATPS
                     GIHPSDSTDPTLMDDWEVGDPGCASTRQGDPCTTAYEWRFRLVQEIRRLFPPTATNIV
                     LIGHSTGARTAFEVASNTGPGGVGTQSWGVQDRIAAVVSINGMIDGLQTSKYNVVGFS
                     DFVSTCKNSDAIAAVFGAGVPGNGWCEYAGNVSGIAAADWVGQHKRARVLTAWGSCSP
                     SLWTGNSDGALPYAAQGSPSSPFLDMTPAAGQTFTVAYGRRFGAFCHSTITSGSDANH
                     AAAVASARSQIEDLLFTSAVRVDQTGTISTSSPVPFNGSTPTFNLGGACPAGDVAISP
                     VVQVVGVCKHPGLFDGDDHAITNGFTVTDGTGCAGAFRWAQIHDSSNSHNATFFWQTR
                     SRPADGGLIATLPPG"
     gene            complement(1744..>2347)
                     /locus_tag="VF516_40875"
     CDS             complement(1744..>2347)
                     /locus_tag="VF516_40875"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEX8114174.1"
                     /translation="KALAALAAFAIMALAGVSLAGAAMSAAAALMVVARTPPRRAFAS
                     VDWQLLVFFAGLFVVVAGIAHTGVLDRLFEQLAPMIARGDAVGDLAFVALVVLASNLV
                     SNVPLVLVAVSWVPRMPDPTWGYIMLAVASTLAGNLTLFGSVANVIVMESAGPRGEIG
                     FWRFLRYGAVITTVDLAVAFGLRAAERALGVPGWRGLGGS"
BASE COUNT          358 a          871 c          843 g          275 t
ORIGIN      
        1 cggccacgag cccgggctgt ccgcgctcgg cgcggtgctg gcaggccggc ccgagttcgc
       61 cagcctggcc aaggcccagg ccgcccggat cgacgacggg cagctgcgct ggcggttcgc
      121 gtgcgacgcc gaggcgcccg agatcatcga ctagatcggg cctgatcggg cctgatcggg
      181 cctgatcggg cgtagccgga gctcctaccc aggggccgcg gggctcagat cgtgcgagca
      241 cagccgctga caccggggcc ggcccacgcc gtccggcgag cagtgcctgc aaccaggtga
      301 tggcgccggg tcgccgacgc cgtccgcccg gcgtgatgcg atgcgggcca tgcgtcacgc
      361 gctgctcgcc gccatcgcct gctccgccgt gcccgggtgc accaccaccg agccgccgct
      421 cgagccggcc gtggccgcgg cggtgaccgg cacggtccag ctcaacctgc tctacatcca
      481 cggcgtcgag aacagcgcga gcgcccggac cggcgcgcac aacacgctga acgacctcaa
      541 ggccgcggtc gccgccgacc tggcgtcgct gatcgcgagc taccaggcca gccaccccgg
      601 ggtgacgatc cacgccgcga gcgcgtcggc caacctctac accgcgacgc cgtcggggat
      661 ccaccccagc gactcgaccg atccgacgct gatggacgac tgggaggtcg gcgatccggg
      721 ctgcgcgtcg acccgccagg gcgatccgtg caccaccgcc tacgagtggc ggttccggct
      781 ggtccaggag atccggcggc tgttcccgcc gaccgcgacg aacatcgtcc tgatcggcca
      841 ctcgaccggc gcgcgcaccg cgttcgaggt cgcgtcgaac accgggcccg gcggcgtcgg
      901 cacccagagc tggggcgtcc aggaccggat cgccgcggtg gtgtcgatca acggcatgat
      961 cgacggcctg cagaccagca agtacaacgt ggtcgggttc agcgacttcg tgtcgacgtg
     1021 caagaacagc gacgcgatcg cggcggtgtt cggcgccggc gtgccgggca acggctggtg
     1081 cgagtacgcc ggcaacgtca gcggcatcgc cgccgccgac tgggtcggcc agcacaagcg
     1141 cgcgcgggtg ctgaccgcgt ggggcagctg ctcgccatcg ctgtggaccg gcaacagcga
     1201 cggcgcgctg ccgtacgcgg cgcagggcag cccgtcctcg ccgttcctcg acatgacgcc
     1261 cgccgccggt cagaccttca cggtcgcgta cggccggcgg ttcggcgcgt tctgtcactc
     1321 gacgatcacc agcggcagcg acgccaacca cgccgcggcg gtcgccagcg ccaggtcgca
     1381 gatcgaggat ctgctgttca cctcggcggt ccgcgtcgac cagaccggca cgatctcgac
     1441 gtcgtcgccg gtgccgttca acggctcgac cccgacgttc aacctcggcg gggcatgccc
     1501 ggccggcgac gtcgcgatct cgccggtcgt ccaggtcgtc ggcgtgtgca agcacccggg
     1561 gctgttcgac ggcgacgacc acgcgatcac caacggcttc acggtgaccg atggcaccgg
     1621 ctgcgcgggc gcgttccgct gggcgcagat ccacgactcc agcaacagcc acaacgcgac
     1681 gttcttctgg cagacccggt cgcggccggc cgacggcggc ctgatcgcga cgctgccgcc
     1741 gggctagctt ccgccaaggc cgcgccagcc cggcacgccg agcgcgcgct cggccgcgcg
     1801 caggccgaac gcgacggcga ggtcgacggt ggtgatcacc gcgccgtagc gcaggaaccg
     1861 ccagaacccg atctcgccgc gcggcccggc gctctccatc acgatgacgt tggcgacgct
     1921 gccgaacagc gtcaggttgc cggccagcgt cgacgcgacc gcgagcatga tgtagcccca
     1981 ggtggggtcc ggcatgcgcg ggacccacga cacggcgacc agcacgagcg ggacgttgga
     2041 caccaggttg gaggcgagca cgacgagcgc gacgaacgcg aggtcgccga ccgcgtcgcc
     2101 gcgcgcgatc atcggggcga gttgctcgaa cagacggtcg agcacgccgg tgtgggcgat
     2161 cccggcgacc acgacgaaca ggccggcgaa gaacaccagg agctgccagt cgacgctggc
     2221 gaacgcgcgg cgcggcgggg tgcgcgcgac gaccatcagc gcggcggcgg cggacatcgc
     2281 ggcaccggcc agcgacaccc cggcgagcgc catgatcgcg aacgcggcga gcgcggccag
     2341 cgccttg
//
LOCUS       DASVOR010002204         3632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_140510,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002204 DASVOR010000000
VERSION     DASVOR010002204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140510"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VF516_40880"
     CDS             <1..473
                     /locus_tag="VF516_40880"
                     /inference="COORDINATES: protein motif:HMM:NF024959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8114175.1"
                     /translation="VSEGAGDPLATQRRHAAPISALEIRGNTLFSFSLDQSMRRWTLP
                     SGEPRERAYMRFDGSAMSPSGEWIATVSGLPAVSLWDSAKGRLLWQLPAGEPLTSVVF
                     LDDDHVVVGGKTGRLEILDVSGRRGPPRTASEVIRRVGESPRWHVENGRVVERQ"
     gene            complement(502..1617)
                     /locus_tag="VF516_40885"
     CDS             complement(502..1617)
                     /locus_tag="VF516_40885"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEX8114176.1"
                     /translation="MSANASETILVHLHPDFQQPLEELFDSWNQIQSSWNFVGIRPRR
                     EYERALLTPGAISSDDASQLAARIRAAVGYTSEPSVIVFTEKRLFDDKYDQLFLGGQE
                     ADEDPPSIGIVSLEFLRRNDLQRLLGPVLFRAVISNILYSLGVDSGLDDHHDETRGCV
                     MDFCNYMPDIVVGLREGPRFCAACASTLGRGTGRDLVRLADRARNQAGLISTDETVTE
                     SIVVRGKRYANTINKFDYDVALSFAGPDRDHAEQLAIALRDLGLEVFYDLFDQEKLWG
                     RNLHPYLTELYRIRARYCVVFLSAHYKRSKWTRVELDAALAREFEEGSEYILPIRLDD
                     AQIPGLLSTRAYLDWHEYSAAAVADMVKKRLVEAEDS"
     gene            complement(1664..2476)
                     /locus_tag="VF516_40890"
     CDS             complement(1664..2476)
                     /locus_tag="VF516_40890"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEX8114177.1"
                     /translation="MTHRHTVLPVAAAFALTAFAPTAVPAQPAPAKKPVDPRAQAIAD
                     VVVANHILADQGVVDGFGHVSIRDPSDPNKFFLARSMAPELVTTADVFEHDLDGNAPT
                     AGSAKLYLERFIHAEIYRARPDVNAIVHCHAPDLIPFGAAGVALRPLYHMSSFLGAGV
                     PVFDIHKAANASTDMLIRSSDLGKALAKSLGPHPAALMRGHGAVIVGKDVQAAVFRSV
                     YTELNAKLQAQAMTLGSKVIYLDADEAKKSEETNAALVGRSWELWVRKVRDK"
     gene            2581..3624
                     /locus_tag="VF516_40895"
     CDS             2581..3624
                     /locus_tag="VF516_40895"
                     /inference="COORDINATES: protein motif:HMM:NF016115.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004061 - arylformamidase activity
                     [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEX8114178.1"
                     /translation="MAQRWKHRPPGSSWGEFGPDDQRGRMNYVTADKVRQGVAEVKEG
                     KTFCLSLPLDVPGGSVLNPRRGPPRLAPTVRSGKQNFCFAASDEDPLLTDVVCDDVVL
                     LTLQYSTQWDSLAHVGSRFDADGDGVAEPVFYNGFRAGEDIRPGPEHPGAEPWARFEG
                     SRADALGIANLAEHGAQGRGVMIDLHAHFGRERKAIGYDDLMRVMDADGVVVEQGDLV
                     CLHTGFADLLLEQDRRPDPAVVHHSCTGLEGRDTRLLDWISESRCAALIADNYAVELL
                     PTTPYRPTPHAYLPLHEHCLFKNGIHLGELWHLGELARWLRAHGRSRFLLTAPPLRLP
                     GAVGSPAAAVATV"
BASE COUNT          576 a         1173 c         1293 g          590 t
ORIGIN      
        1 gggtctccga gggtgcgggc gacccgctgg ccacgcagcg gcgccacgcc gcgccgatca
       61 gcgccctgga gatccggggc aacacgctgt tctcgttcag cctggaccag tcgatgcgcc
      121 ggtggaccct cccctccggc gagccacgcg agcgtgcgta catgcggttc gacggctcgg
      181 cgatgtcccc gagcggcgag tggatcgcga cggtgagcgg gctacccgcc gtgagcttgt
      241 gggacagcgc gaagggccgc ctcctctggc agctcccggc cggcgagccg ctgaccagcg
      301 tcgtgttcct cgacgacgat cacgtcgtcg tgggcggcaa gaccggccgc ctcgagatcc
      361 tcgatgtgtc cggacggcgc ggaccgccgc gcacggcgtc cgaggtgatt cgccgggtcg
      421 gcgagtcgcc gcggtggcac gtggagaacg ggcgcgtggt cgagcggcag tagcccccgg
      481 ggttgccgcc gagaggtgat gtcagctgtc ctcggcttcg accagccgct tcttgaccat
      541 gtcggccacc gcagccgccg aatactcgtg ccagtcgaga taggctcgcg tggagaggag
      601 tcctggtatc tgggcgtcgt ccagccgtat cggcaggata tattcgctgc cctcttcgaa
      661 ctcgcgtgcc agtgccgcat cgagctcgac acgcgtccat ttgctacgct tgtaatgggc
      721 cgaaaggaag accacacaat atctggctct gatacggtac agctcggtca gataagggtg
      781 aaggttcctt ccccacagct tctcctgatc gaacagatcg taaaacacct cgaggccgag
      841 atcccgcaag gcgatcgcca gctgctctgc gtggtcacga tctggacccg cgaacgagag
      901 agccacgtca tagtcgaact tgttgatcgt gttcgcatag cgcttgccac gaaccacgat
      961 ggattccgtg accgtttcat ccgtggagat gagtccggcc tgattgcgcg cgcggtcggc
     1021 gagcctgacg aggtctcgtc cagtcccccg gcccagggtg cttgcgcagg ccgcgcagaa
     1081 tcgcggaccc tcgcgcaatc cgacgacgat gtcgggcatg tagttgcaga agtccatgac
     1141 acagccgcgg gtctcgtcgt gatggtcatc caaccccgag tcgaccccga gcgagtacaa
     1201 gatattcgag atgacggctc gaaacagaac gggaccgagg agtcgctgga ggtcgtttct
     1261 tcgcaagaac tccagtgaaa cgataccaat gctgggcgga tcctcgtcgg cttcttgccc
     1321 tccgagaaag agttgatcat acttgtcgtc aaatagccgc ttctcggtaa agacgatgac
     1381 actgggctcg gatgtgtagc caacggcagc ccggattctt gcggcgagct gcgaagcatc
     1441 gtcgctggag atcgcgcccg gtgtcagcag tgctcgttcg tattcgcgac gaggtcgaat
     1501 gccgacaaag ttccatgagc tctggatctg attccaggaa tcgaacagct cttcgagcgg
     1561 ctgctgaaag tctggatgca gatgcaccag gatggtctcg ctcgcattcg cagacatgat
     1621 cggtgctccc gtgcgtcccg gtccgcgcct caccgcggag ccgctacttg tcgcggacct
     1681 tgcggaccca gagctcccac gatcggccga ccagcgcggc gttggtctcc tcggacttct
     1741 tggcctcgtc ggcgtcgagg tagatcacct tggacccgag cgtcatcgcc tgggcctgca
     1801 gcttggcgtt gagctcggtg tagaccgagc ggaacacggc cgcctggacg tccttgccga
     1861 cgatcaccgc gccgtggccg cgcatcagcg ccgccgggtg ggggccgagc gacttggcga
     1921 gcgccttgcc gaggtcggac gaccggatca gcatgtcggt cgacgcgttg gccgccttgt
     1981 ggatgtcgaa caccggcacg ccggcgccga ggaacgagct catgtggtag agcgggcgga
     2041 gcgcgacgcc ggcggcgccg aacgggatga gatccggcgc gtggcagtgc acgatcgcgt
     2101 tgacgtcggg ccgcgcgcgg tagatctcgg cgtggatgaa gcgctcgagg tagagcttgg
     2161 cgctgccggc ggtgggggcg ttgccgtcga ggtcgtgctc gaagacgtcg gcggtggtga
     2221 ccagctccgg agccatgctg cgcgccagga agaacttgtt gggatcgctg ggatcgcgga
     2281 tgctgacgtg gccgaagccg tcgaccacgc cctggtcggc gaggatgtgg ttggcgacga
     2341 cgacgtcggc gatcgcctgg gcccgcggat cgacgggctt cttggcaggc gccggctggg
     2401 ccgggacggc ggtgggtgcg aacgcggtca gggcaaacgc cgccgcgacg ggcaggacgg
     2461 tgtggcgatg ggtcatgggg ctcctcggga gggcgggcgg cgccgaacgt acgcgaaacc
     2521 ggcggccgcg cacagcggga ttcgcagcgg cgcgggcagg tggcttatag tcgccgcgcc
     2581 atggcgcaac gctggaagca cagaccgccc ggctcgagct ggggtgagtt cggccccgat
     2641 gatcagcgcg gccggatgaa ctacgtgacg gcggacaagg ttcggcaggg cgtggccgag
     2701 gtcaaggagg gcaagacgtt ctgcctgtcg ctgccgctcg acgttccggg gggcagcgtg
     2761 ctgaacccgc ggcgcggccc gccgcggctg gcaccgacgg tgcgcagtgg caagcagaac
     2821 ttctgcttcg ccgcctccga cgaggatcca ctgctgaccg acgtggtgtg cgacgacgtc
     2881 gtgctgctca ccctgcagta ctcgacgcag tgggactcgc tggcccacgt cggcagccgg
     2941 ttcgacgccg acggcgacgg cgtggccgag ccggtgttct acaacgggtt ccgcgccggc
     3001 gaggacatcc ggcccgggcc ggagcacccc ggcgccgagc cgtgggcccg gttcgagggc
     3061 tcgcgcgccg acgcgctcgg catcgccaac ctcgccgagc acggggcgca gggccgcggt
     3121 gtgatgatcg acctgcacgc ccacttcggc cgcgagcgca aggcgatcgg ctacgacgac
     3181 ctgatgcgcg tgatggacgc cgacggcgtg gtcgtcgagc agggcgacct ggtgtgcctg
     3241 cacaccgggt tcgccgacct gctgctcgag caggaccgcc ggccggatcc cgcggtcgtc
     3301 catcacagct gcaccgggct cgagggccgc gacacccggc tgctcgactg gatctcggag
     3361 agccgatgcg cggcgctgat cgcggacaac tacgcggtgg agctgttgcc gacgacgccg
     3421 taccgcccga cgccgcacgc gtacctgccg ctccacgagc actgcctgtt caagaacggc
     3481 atccacctcg gcgagctgtg gcacctcggc gagctggcgc gctggctgcg cgcgcacggc
     3541 cgcagccggt tcctgctgac cgcaccgccg ctccgcttgc ccggcgcggt cggctcgccg
     3601 gcggcggcgg ttgccacggt ataggcgttc ag
//
LOCUS       DASVOR010002205         2683 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_491472,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002205 DASVOR010000000
VERSION     DASVOR010002205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491472"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1229)
                     /locus_tag="VF516_40900"
     CDS             complement(147..1229)
                     /locus_tag="VF516_40900"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEX8114179.1"
                     /translation="MHRSLLVRLLVRLLVPPLAACALGTLASRASADPSPVPTVEVTA
                     GRVEVDHAGAIPTDLLFEPGDAQIKPAARPLLDAVARALAKDPTTMVRIACHTDDTAP
                     DNDRTGEYNLRLSQQRADAVLGYLARHGLAARRLTARGFGRDQPAGDNATDEGKRANR
                     RLELRVAGEVRPPDSSDLAAYTRAIKGKGALIATIATSQGTLHCQLFDDKAPATVANF
                     VGLATGQKPWTDPRTGKTVRGKPFYDGLSFHRVIPRFMIQGGDPLNNGTGGPGYHFDD
                     EVAPDLTHQPGTLAMANAGPGTSTNGSQFFIDEVEASWLKGYTIFGKCKEVDVVSRIA
                     GVPRGAADKPELPVAITRLTIARGEL"
     gene            1302..>2683
                     /locus_tag="VF516_40905"
     CDS             1302..>2683
                     /locus_tag="VF516_40905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8114180.1"
                     /translation="MPAATTNPLIDDRDVELILDEVLDLGRLLRLPYFADHDRETCEL
                     LLASARELARDQLYPAYRALDAEPPRLVDGRVVVHPRMRALYGRMTELGLVAAPRPHA
                     VGGSQVPLAVFSLATAYLMAGNLSAYGYLGLTQGAAHLLEAFGDDALRAAYMAPLYRG
                     EWTGTMALTEPHAGSSLADITTTAEPTADGHYLVRGAKIFISGGDHDLTDNIVHMTLA
                     RITGAPAGMKGVSLFCIPKRRLQHGALVDNDVAVTGLIHKIGWRGLPSLALAYGERGD
                     CRGWLVGEPHQGIRYMFQMMNEARIMVGLNGIATASVAYHEAVAYARTRTQGRPLSAK
                     DPAVPPVAIARHPDVRRMLLRQKAIAVGGLVLIARVSSYADLALHASDAAERERAGLL
                     LDLLTPVAKTFPAERGFEANALAVQIHGGYGYSSEYLPEAWLRDQKLNSLHEGTTGIQ
                     SIDLLGRKVS"
BASE COUNT          356 a         1012 c          946 g          369 t
ORIGIN      
        1 gagccggccg accgatgacc tgccgggcgg ggccaaccac gccgcggcgg gcgccgccgc
       61 ccgcaagctg cagaccgcga tgaccacctg cgccggcacg accagcgtcg cggccgcgat
      121 gaaggatctg ccgggtccct agcgcgtcac agctcgccgc gcgcgatcgt gagccgcgtg
      181 atcgcgaccg ggagctcggg cttgtcggcg gcgccgcgcg gcacgccggc gatccggctg
      241 accacgtcga cctccttgca cttgccgaag atcgtgtagc ccttgagcca gctcgcctcg
      301 acctcgtcga tgaagaactg gctgccgttg gtgctggtgc ccggcccggc gttggccatc
      361 gccagcgtcc cgggctggtg ggtgaggtcg ggcgcgacct cgtcgtcgaa gtggtagccc
      421 gggccgccgg tgccgttgtt cagcggatcg ccgccctgga tcatgaagcg cgggatcacg
      481 cggtgaaagc tcagaccgtc gtagaacggc ttgccgcgca cggtcttgcc ggtccgcggg
      541 tcggtccacg gcttctgacc ggtcgccagg ccgacgaagt tggcgaccgt cgccggcgcc
      601 ttgtcgtcga acagctggca gtgcagcgtg ccctggctgg tcgcgatcgt cgcgatcagc
      661 gcgcccttgc ccttgatcgc ccgggtgtag gccgccaggt ccgagctgtc cggcggccgg
      721 acctcgcccg cgacccgcag ctcgagccgc cggttggcgc gcttgccctc gtcggtggcg
      781 ttgtcgccgg ccggctggtc gcgcccgaag ccccgggcgg tcagccgccg ggcggccagc
      841 ccgtgcctgg ccaggtagcc cagcacggcg tcggcgcgct gctggctgag ccgcaggttg
      901 tactcgccgg tgcggtcgtt gtcgggcgcg gtgtcgtcgg tgtggcacgc gatccgcacc
      961 atcgtcgtcg ggtccttggc cagcgcgcgc gcgacggcgt cgagcagcgg ccgcgccgcc
     1021 ggcttgatct gggcgtcgcc cggctcgaac agcaggtcgg tcggaatcgc cccggcatgg
     1081 tcgacctcga cccggcccgc ggtgacctcg acggtgggga ccggcgacgg gtcggcggac
     1141 gcacgcgacg cgagcgtccc cagggcacag gcagcgagcg ggggaacgag caggcgaacg
     1201 agcaggcgaa cgagcaggct gcggtgcatg gccgggattg tgaaccaggc cgctcacgcg
     1261 gtcgacccca cgggccggtc gccgtgcgac gatgcggcgc gatgcccgcc gccacgacca
     1321 acccgctgat cgacgaccgc gacgtcgagc tgatcctcga cgaggtgctc gatctcggcc
     1381 ggctgctccg gctgccgtac tttgccgacc acgatcgcga gacctgcgag ctcctgctcg
     1441 cctcagcgcg cgagctcgcc cgcgatcagc tctatccggc ctaccgcgcg ctcgacgccg
     1501 agccgccgcg cctggtcgac ggccgcgtcg tcgtccatcc ccggatgcgc gcgctgtacg
     1561 ggcggatgac cgagctcggc ctggtcgccg cgccccggcc gcacgcggtc ggcggctcgc
     1621 aggtcccgct cgcggtgttc tcgctcgcga ccgcctacct gatggccggc aacctgtcgg
     1681 cctacggcta cctcgggctg acccagggcg ccgcccacct gctcgaggcg ttcggcgacg
     1741 acgcgctgcg cgccgcgtac atggcgccgc tgtaccgcgg cgagtggacc ggcacgatgg
     1801 cgctgaccga gccccacgcc gggtcgagcc tcgccgacat cacgaccacc gccgagccga
     1861 ccgccgacgg ccactacctg gtgcgcggcg ccaagatctt catctcgggc ggcgatcacg
     1921 acctcaccga caacatcgtc cacatgaccc tcgcccggat caccggcgcg ccggccggca
     1981 tgaagggcgt gtcgctgttc tgcatcccca agcggcggct tcagcacggc gcgctggtcg
     2041 acaacgacgt cgcggtcacc ggcctgatcc acaagatcgg ctggcgcggc ctgcccagcc
     2101 tcgcgctggc ctacggcgag cgcggcgact gccggggctg gctggtcggc gagccccacc
     2161 agggcatccg ctacatgttc cagatgatga acgaggcgcg gatcatggtc gggctcaacg
     2221 gcatcgcgac cgcatcggtc gcgtaccacg aggcggtggc ctacgcccgg acccgcaccc
     2281 agggccggcc gctgtccgcc aaggaccccg ccgtgccgcc ggtggcgatc gcccgccacc
     2341 ccgacgtccg ccgcatgctg ctgcgccaga aggcgatcgc ggtcggcggg ctggtgctga
     2401 tcgcgcgggt gtcgtcctac gccgacctcg cgctgcacgc ctccgacgcg gccgagcgcg
     2461 agcgcgccgg cctgttgctc gacctcctca ccccggtcgc caagacgttc cccgccgagc
     2521 gcggcttcga ggccaacgcg ctcgcggtgc agatccacgg cggctacggc tactcgagcg
     2581 agtacctgcc ggaagcctgg ctccgcgacc agaagctcaa cagcctccac gaaggcacca
     2641 ccggcatcca gagcatcgac ctcctcggac gaaaagtgag cat
//
LOCUS       DASVOR010002206         3216 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_520050,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002206 DASVOR010000000
VERSION     DASVOR010002206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520050"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..282)
                     /locus_tag="VF516_40910"
     CDS             complement(10..282)
                     /locus_tag="VF516_40910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114181.1"
                     /translation="MENRARNARHSRDPHTRMIGKLCLGGDWACANGDLGALGDIADR
                     LITCSPETLHCELARLSALCRHDPDRATAAWVQLKNRVLGSVAAPS"
     gene            complement(417..734)
                     /locus_tag="VF516_40915"
     CDS             complement(417..734)
                     /locus_tag="VF516_40915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114182.1"
                     /translation="MRFAPEVLARLRRDLMRRGLTLATLLSQVLAGKRPPELAALLAQ
                     RPGKRPEEVLRLALDQVEARRKLLDSGDDRYGRCDTCGVDLGAAALGEMPWADRCPAH
                     AAM"
     gene            complement(745..1671)
                     /locus_tag="VF516_40920"
     CDS             complement(745..1671)
                     /locus_tag="VF516_40920"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEX8114183.1"
                     /translation="MQALPYAIILAGGEGQRLAPLTRALYGTDLPKQFAVLSGERSLM
                     QTTIERAAALTTLDRISVVVTAHHEKIARAQLAPYPGVELVIQPRNLDTGPGLLLPLV
                     HILARAGSARVVFLPSDHHVSNPAPVAEALCHAGEDGLDDRISLLGVTPTGPEVEYGW
                     IVRGARVARTGGYTVRRFLEKPTHAVAEDLWRSGGLWNTFISSGPAHLFWTLARRHLP
                     SHTERLERYATAIDGADEAEALHAAYDGMAPANFSRSVLANADALAVLPVAGSGWCDW
                     GSPQRVLASLAGTDGHARLVARMGGDTAVAVS"
     gene            complement(1986..2594)
                     /locus_tag="VF516_40925"
     CDS             complement(1986..2594)
                     /locus_tag="VF516_40925"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEX8114184.1"
                     /translation="MTYPVQLEVTSPVRFDRVQLALRVVLAIALGWVGVSAGWLGCAL
                     FLALPVIAAVVISTRGAQAYLDNTGPKVWQVVSWLLAFSAYMLLLTDRFPVGEPTGVQ
                     VQIRPDGHPTTSSALLRLITSLPSGLVLALLGCVSSVLFVIGAVMILAEEQVPAWILG
                     FQRGVLRWQARLAGYHASLIDEYPPFSFEADPAQPGDHAHTA"
     gene            complement(2596..2871)
                     /locus_tag="VF516_40930"
     CDS             complement(2596..2871)
                     /locus_tag="VF516_40930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114185.1"
                     /translation="MRATPPSAIAVLEARVTEHPWRTLGTAFLLGAWLGLEPLHVPRS
                     RFARAAFAMVGSLALRVAREIALGNLAEHALRAAPRGPGGTSDAPFH"
     gene            complement(2868..>3216)
                     /locus_tag="VF516_40935"
     CDS             complement(2868..>3216)
                     /locus_tag="VF516_40935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114186.1"
                     /translation="NGLREIGGRTGDRPGTRHAVPRGEERTMPSHNDASSPASLDDRI
                     DALAANLKNLVSQMSTTAAEVKGRAIDVKQRASSSAGSLAARAGRVIQDHPIAAIGVA
                     FGVGYLVMRLIRR"
BASE COUNT          458 a         1171 c         1188 g          399 t
ORIGIN      
        1 gccgcgaggt cacgacggcg ccgcgacgct gcccagcacg cggttcttga gctggaccca
       61 ggccgcggtg gcgcggtccg gatcgtgccg gcacagcgcc gacagccggg ccagctcgca
      121 gtgcagcgtc tcggggctgc aggtgatcag gcggtcggcg atgtcgccga gcgcgccgag
      181 gtcgccgttg gcacacgccc agtccccacc caggcacagc ttgccgatca tccgtgtgtg
      241 cggatcccgg ctgtgccgcg cgttgcgggc ccggttctcc atgttccaag ggcgtacaag
      301 cggcgtgccc gtccggtcgg gcgcggtcgc ccacccgggg tcgcgccggc ctgcatcgca
      361 cgcgtccggg gccggcggga ctgcaagcgc tgcgggatcc ggcggccgcg acccgatcac
      421 atcgcggcgt gggcggggca gcggtcggcc cacggcattt ctccgagggc ggcggcgccc
      481 aggtcgacgc cacaggtatc gcatcggccg tagcggtcgt ccccgctgtc gagcagcttg
      541 cggcgcgcct cgacctggtc gagcgcgagc cggagcacct cctccggccg cttgccgggc
      601 cgctgggcga gcagcgcggc gagctcgggc ggccgcttgc cggcgagcac ctgggacagc
      661 agggtcgcca gggtcagacc gcggcgcatc aggtcgcggc gcagccgtgc caggacctct
      721 ggagcgaagc gcacccgtgc gaggttagct gacggccacc gccgtgtcgc cgcccatccg
      781 ggcgaccagg cgggcgtggc catcggtgcc ggccaggctc gcgagcacgc gctgcgggga
      841 gccccagtcg caccacccgg agccggcgac cggcaggacc gcgagtgcgt cggcgttcgc
      901 cagcaccgag cggctgaagt tggccggcgc catcccgtcg tacgccgcgt gcagcgcctc
      961 ggcctcgtcg gcgccgtcga tcgcggtcgc gtagcgctcg agccgctcgg tgtggctcgg
     1021 caggtggcgc cgcgccaggg tccagaacag gtgcgccggg ccgctggaga tgaacgtgtt
     1081 ccacagcccg ccgctgcgcc acaggtcctc ggcgacggcg tgggtcggct tctccaggaa
     1141 ccggcggacc gtgtagccgc cggtgcgggc gacccgcgcg ccgcgaacga tccagccgta
     1201 ctcgacctcg gggccggtcg gcgtcacgcc cagcagcgag atccggtcgt cgagcccgtc
     1261 ctcgccggcg tggcacagcg cctcggcgac cggcgccggg ttcgacacgt ggtgatccga
     1321 cggcaggaac acgacccggg ccgagccggc gcgcgcgagg atgtgcacca gcgggagcag
     1381 cagcccgggg ccggtatcga ggttcctggg ctggatcacc agctccactc ccggataggg
     1441 cgcgagctgg gcccgcgcga tcttctcgtg gtgcgcggtc acgaccaccg agatccggtc
     1501 cagggtggtg agggcggcgg cgcgctcgat cgtggtctgc atcagcgagc gctcccccga
     1561 cagcaccgcg aactgcttcg ggaggtcggt gccgtacagc gcgcgggtga gcggcgcgag
     1621 gcgctggccc tcgccgccgg ccaggatgat cgcgtaaggg agcgcctgca tcgggacgga
     1681 acgctctgca ccctgtgcgc caaccgcgga tgcaggaaag caccgggtaa acgcgcacgc
     1741 cgtgcgcctg gggcagcatg gaccgcacgc cgtgcgcact cgcaacgcgc ccgatcgccc
     1801 gtcggtacgg cgggctacca atgcccgggg cactccggat ccgagtacgc gtccgacgcg
     1861 gcgagacacc gcgtcgcatc gcgagcaggc ggaccaaaac cctgtccagg gtggccaggg
     1921 gatcgatcca gccctgttcc tggccctgtt cccagccctg ttcgtgacga tccgggccgc
     1981 ggcggtcagg ccgtgtgtgc gtggtccccc ggctgcgccg ggtcggcctc gaacgagaac
     2041 ggcgggtact cgtcgatcag cgacgcatgg tagccggcga gccgggcctg ccagcgcagc
     2101 acgccgcgct ggaacccgag gatccaggcc gggacctgct cctcggccag gatcatcact
     2161 gcgccgatca cgaacagcac ggacgacacg cagccgagca gcgcgagcac caggccgctc
     2221 gggagtgacg tgatcagccg gagcagcgcg gagctggtgg tggggtggcc atcgggccgg
     2281 atctggacct gcacgccggt gggctcgccg accgggaacc ggtcggtgag cagcagcatg
     2341 taggccgaga acgcgagcag ccagctcaca acctgccaca ccttcggccc ggtgttgtcg
     2401 aggtaggcct gggcgccccg ggtcgagatc acgaccgcgg cgatcaccgg cagcgccagg
     2461 aacagcgcgc atcccagcca gcccgccgac acgccgaccc agccgagcgc gatcgcgagc
     2521 accacacgca gcgccagctg gacgcgatcg aaccgcaccg gcgaggtgac ttcgagctgg
     2581 acggggtagg tcatgtcagt ggaacggtgc atcgctcgtg ccgccgggtc cgcgcggcgc
     2641 cgcccgcagc gcgtgctcgg cgagattgcc gagcgcgatc tcgcgcgcca ctcgcagggc
     2701 gagcgagccg accatcgcga aggccgcgcg cgcgaaccgg ctgcgcggga catgcagcgg
     2761 ctccagcccg agccatgcgc cgagcaggaa cgccgtgccc agcgtccgcc acgggtgctc
     2821 ggtcacgcgc gcctcgagca ccgcgatcgc cgacggcggt gtagccctca tcgccggatc
     2881 agccgcatca cgaggtagcc gacgccgaac gccacgccga tcgccgcgat cgggtgatcc
     2941 tggatcaccc ggcccgcccg ggccgccagc gagcccgcgc tcgagctcgc gcgctgcttg
     3001 acgtcgatcg cccggccctt gacctcggcg gcggtcgtcg acatctgcga caccaggttc
     3061 ttgaggttcg ccgcgagcgc gtcgatccgg tcgtcgagcg acgccggaga tgaagcatcg
     3121 ttgtgggaag gcatggttcg ctcctcgcca cgaggcacag catgccgcgt gcccggccgg
     3181 tcgccggtcc ggccgccgat ctctcgcaaa ccgttc
//
LOCUS       DASVOR010002207         1560 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_432188,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002207 DASVOR010000000
VERSION     DASVOR010002207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432188"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VF516_40940"
     CDS             complement(<1..784)
                     /locus_tag="VF516_40940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114187.1"
                     /translation="MIHIALVPYQVSIDPNQLTRVAAALQRQVHEQFGPLWQIEATVS
                     PFVDWNAVPPDYTRLIVVDHLDSTALGVHADKDGKPYALVNAHGSWPLVASHELLEML
                     ADPTGQQTRPGPSPKQDGKTVDILVEVCDPCQGSRWSYEIDGIPVSEFCTPAFYGGGP
                     GPWSYRGNVKAAHQVLKDGYLIWHDPAAQLWWRRDYLGSEPTDYALGPMPPTVSFLRG
                     HIDRVTRGHRQAHHQTHRRKRPAAAPVAPVSPAAKARSRALAK"
     gene            1037..>1560
                     /locus_tag="VF516_40945"
     CDS             1037..>1560
                     /locus_tag="VF516_40945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8114188.1"
                     /translation="MDLRYTAEEQQFRTEVRSFIQANLPSDISDRVLRGKHLQREDWL
                     RWHRLLHQRGWSTPNWPKEFGGPGWNAAQQHIFEDECADAGAPPLMPFGIRMVAPVIM
                     AFGNRVQQDRFLPRIRSGDHWWCQGYSEPGSGSDLASLRMRAERRGDVYICNGQKTWT
                     TLAQHADWIFCLVRT"
BASE COUNT          230 a          538 c          553 g          239 t
ORIGIN      
        1 gcttggccag cgcgcgcgag cgcgccttcg ccgccgggga gaccggcgcg accggggccg
       61 cggcggggcg cttgcgccgg tgggtctgat ggtgggcctg ccggtggccc cgggtgactc
      121 gatcgatgtg tccgcgcagg aagctgacgg tgggcggcat cgggcccaga gcgtaatcgg
      181 tcggctcgct gccgaggtaa tcgcgccgcc accataactg cgccgccggg tcatgccaga
      241 tcaggtagcc gtccttgagc acctggtggg ccgccttgac attgccgcgg tacgaccacg
      301 gcccgggccc gccgccgtag aacgccggcg tgcagaactc cgagaccggg atgccgtcga
      361 tctcgtacga ccaccgcgag ccctggcacg gatcgcagac ctcgacgagg atgtcgacgg
      421 tcttgccgtc ctgcttgggc gacgggccgg ggcgggtctg ctggccggtc ggatcggcga
      481 gcatctccag gagctcgtgg ctcgcgacca gcggccagct gccgtgcgcg ttgaccagcg
      541 cgtatggctt gccgtccttg tcggcgtgga cgccgagcgc cgtcgagtcg aggtgatcga
      601 cgacgatcag ccgcgtgtag tccggcggca cggcgttcca gtcgacgaac gggctgacgg
      661 tggcctcgat ctgccacagc gggccgaact gctcgtggac ctggcgctgc aacgcggcgg
      721 cgacgcgggt gagctggttc ggatcgatcg atacctggta cggcacgagg gcgatgtgga
      781 tcacgacgac tccctgggct ctccaccggc gcggcggcag cgtcgcggag cccgcacgcc
      841 gtgatgacat cgccacgccc gcacggatcg caacatccta acatgccgtc cgcggacccc
      901 ttctggcgcg gccggagagt gccgtgcggt gccggccaca cctacaccta cggcaaactc
      961 ataccactgg ttaccgactg gtcgtttcgt gccgcgcgag ttgtgcgtgc cgagacttcc
     1021 ggtctacgca acagggatgg acctccgcta cacggccgaa gagcagcagt tccggaccga
     1081 ggttcgcagc ttcatccagg ccaacctgcc gtccgacatc agtgacaggg tgctgcgcgg
     1141 caagcacctc cagcgtgagg attggctgcg ctggcaccgc ctcctccatc agcgcggctg
     1201 gagcacgccg aactggccca aggagttcgg cggcccgggc tggaacgcgg cgcagcagca
     1261 catcttcgag gacgagtgcg ccgacgccgg cgcgccgccg ctgatgccgt tcggcatccg
     1321 catggtcgcg ccggtgatca tggcgttcgg caaccgcgtc cagcaggacc gcttcctgcc
     1381 ccggatccgg tccggcgatc actggtggtg ccagggctac tccgagcccg gctcggggtc
     1441 cgatctggcg tcgctgcgga tgcgcgccga gcgccgcggc gacgtctaca tctgcaacgg
     1501 ccagaagacg tggaccaccc tggcgcagca cgccgactgg atcttctgcc tggtccgcac
//
LOCUS       DASVOR010002208         3533 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_461941,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002208 DASVOR010000000
VERSION     DASVOR010002208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3533
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_461941"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="VF516_40950"
     CDS             complement(<1..780)
                     /locus_tag="VF516_40950"
                     /inference="COORDINATES: protein motif:HMM:NF012879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEX8114189.1"
                     /translation="MPKPFANAESPPAAEPVTGPVAKPQALVFPDEAFRARPPVAGAP
                     RPFRLPPVQVFTLASGIQVYLVEQHTLPLVSMDLSFDGGAAVDPPGKEGLASVCMAML
                     TEGTEQLDKLAYAERLADTASTIGGYAGDDSVGLTLSSLARHLDATFAQLADTLCAPG
                     FRRPDFDRMIRRRIEGIRQARRSPASVASRVAGTVLYGPAHPFGAVVTEASLAALTLD
                     DCREFARTWLRPGGAQLFVVGDLTAAAVRACFDSPRLAGWTG"
     gene            complement(861..2159)
                     /locus_tag="VF516_40955"
     CDS             complement(861..2159)
                     /locus_tag="VF516_40955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114190.1"
                     /translation="MTATPDAGPGGGGPGSDGGGTGTTRVQLTVLSTVLDGQPDTGAI
                     AVFQDPSGTMVGDGLVDAAGHAQADMPAGGSITVIRVVDPSDTARDVAMTTIRGVQPG
                     DSITVGSTASPAAFTGGSSTMTASFTPFAANYTYMFYTTCGGVGGSGGTTQLAFVDGC
                     HGATFDLLSVATSTTDPPDVRYIYQTGLTYAANGAVTVPNTWSSMASFTATLTNVPGN
                     LSDLELTHATLLGKIAAAPVTTGVTSPSAGVVAATTSYPPAVGNGAITTLALHNSNAS
                     GFQTLEVRTQTVAATLGIDLAQQALPWLVGAPTGTLTGASWDQLEGGAPDVRLVTWNG
                     HWTDGVRTVFVSWTFEDGEHTTSIALPALPAKYAAYDPGKASGVVVARPAVTYLDYDA
                     LDGYDAARRYGPNLGDSLTDLGVLTDRPLVRRLSRTPFTR"
     gene            complement(2278..>3533)
                     /locus_tag="VF516_40960"
     CDS             complement(2278..>3533)
                     /locus_tag="VF516_40960"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114191.1"
                     /translation="SSLIGGRYQIMSRIGQGGMGKVYKVKHIHLSRTFALKIISNQVA
                     ETDEARELFYREARFASAMSHPNITTVVDFGEDEKVGMFMVMELVDGEALNRILFREK
                     QLSARRACEIVLQVAEALHYIHKQGVVHCDIKTENILISEEEHEGKRNKTVIKLLDFG
                     LARSLHASRVSTSLSGTPHYVAPERIRGEPASPASDVYGVGILLYELVTGQVPWDGPV
                     QKILAGHLDEKARSPSQLVEGGVDPALETLIFHALAKRPAERHKDMAAFIYELKTVMD
                     MMGFGRRARSGPAKRVVIEQRTSSSPSALHLRDELARVAFDACRLPLALVTPQGVILR
                     ANPAFARFVMGAAIDVEGLPVQTTPLATAWSTAEHDLARTLAGSPVRHILEIDVAAGE
                     SRRLLLWLDAVSVEHVMLGVQPLEL"
BASE COUNT          499 a         1257 c         1276 g          501 t
ORIGIN      
        1 gccggtccag ccggcgagcc gcggcgagtc gaagcaggcg cgcaccgccg ccgcggtcag
       61 gtcaccgacc acgaacagct gggcaccgcc cggccggagc cacgtccggg cgaactcccg
      121 gcagtcgtcg agcgtcagcg ccgcgagcga ggcctcggtg acgaccgcgc cgaacgggtg
      181 cgccgggccg tacagcaccg tgccggcgac ccgcgacgcg accgaggccg ggctgcgccg
      241 ggcctgccgg atgccctcga tccgccggcg gatcatgcgg tcgaagtccg gccggcggaa
      301 ccccggcgcg cacagcgtgt cggcgagctg cgcgaacgtc gcgtcgaggt gccgggccag
      361 gctcgacagc gtcagcccga ccgagtcgtc gccggcgtag ccgccgatcg tcgaggcggt
      421 gtccgccagc cgctcggcgt aggcgagctt gtcgagctgc tcggtgccct cggtcagcat
      481 cgccatgcac acgctcgcca gcccctcctt gcccggcgga tcgaccgccg cgccgccgtc
      541 gaagctgagg tccatcgaga ccagcggcag cgtgtgctgc tcgaccaggt agacctggat
      601 cccgctggcc agggtgaaca cctggaccgg cggtagccgg aacggccgcg gcgccccggc
      661 taccggcggc cgggcgcgga acgcctcgtc ggggaacacc agggcctgag gcttcgcgac
      721 cggtcctgtg accggctctg cggccggcgg gctctcggcg tttgcgaacg gcttgggcaa
      781 gtccgcggac accgtagtcg cagcgcccgc cggacgcaac cgggccgtgt cgacaccgtg
      841 tcgacacgtg tcgagcgcgg ctagcgcgtg aatggcgtcc gcgacagccg gcgaacgagc
      901 ggccggtcgg tcagcacgcc gaggtcggtc agtgaatcgc cgaggttcgg gccgtagcgg
      961 cgcgccgcgt cgtaaccgtc gagcgcgtcg tagtcgaggt acgtcaccgc cgggcgagcg
     1021 accacgacgc cgctggcctt gcccgggtca taggccgcgt acttcgcggg cagcgccggc
     1081 agcgcgatgc tggtcgtgtg ctcgccgtcc tcgaaggtcc agctcacgaa cacggtgcgc
     1141 acgccatcgg tccagtggcc gttccacgtc accagccgga cgtcgggcgc cccgccctcg
     1201 agctggtccc acgacgcgcc ggtcagcgtc ccggtcggcg cgccgaccag ccacggcagc
     1261 gcctgctgcg cgaggtcgat gcccagggtg gccgcgaccg tctgcgtccg gacctcgagc
     1321 gtctggaacc cggacgcgtt gctgttgtgc agcgccagcg tcgtgatcgc gccgttgccg
     1381 accgctggcg ggtacgacgt cgtcgccgcc accacgccgg cgctcggcga ggtgacgccc
     1441 gtggtgaccg gcgcggcggc gatcttgccc agcaacgtgg cgtgcgtcag ctccagatcg
     1501 ctcaggttgc ccgggacatt ggtcaacgtc gcggtgaagc tcgccatcga gctccaggtg
     1561 ttgggcacgg tgaccgcgcc attggccgcg taggtgaggc cggtctggta gatgtagcgc
     1621 acgtcgggcg ggtcggtcgt cgacgtcgcc accgacagca gatcgaacgt cgcgccgtgg
     1681 cacccgtcga cgaacgccag ctgcgtcgtg ccgccgctgc cgccgacccc gccacacgtc
     1741 gtgtagaaca tgtacgtgta gttggccgcg aacggcgtga agctcgccgt catcgtcgag
     1801 ctgccgccgg tgaacgccgc gggcgacgcg gtcgaaccga ccgtgatcga atcgccgggc
     1861 tggacgccgc ggatcgtcgt catcgcgacg tcgcgcgcgg tgtccgacgg atcgaccacg
     1921 cggatcaccg tgatcgaccc accggccggc atgtcggcct gcgcgtggcc ggcggcatcg
     1981 accagtccgt cgccgaccat cgtgcccgac ggatcctgga acaccgcgat cgcgccggtg
     2041 tccggctgcc cgtcgagcac ggtcgacagc acggtgagct gcacccgcgt cgtgccggtg
     2101 ccaccgccat cgctgcccgg cccgccaccg ccggggccgg cgtccggcgt cgcggtcacg
     2161 ctgccgcacc cgcagagcaa cgcacaccac aacaatcgct gcttctggtt ggtcatgggc
     2221 cgcgcggctg caacgcatgt gccacgcgag ctaccgcaca ccgcttcggt gaccggccta
     2281 cagctcgagc ggctggacgc cgagcatgac gtgctcgacg gagaccgcgt cgagccagag
     2341 cagcaggcgg cgggactcgc cggcggcgac gtcgatctcg aggatgtggc gcaccggact
     2401 gccggccagg gtgcgcgcga ggtcatgctc ggcggtgctc cacgcggtgg cgagcggtgt
     2461 ggtctgcacc ggcaggccct cgacatcgat cgccgcgccc atcacgaacc gggcaaacgc
     2521 cgggttggcg cgcaggatca cgccctgggg cgtgaccagc gcgagcggca ggcggcaggc
     2581 atcgaacgcg acccgcgcca gctcgtcgcg caggtggagc gcgctgggcg agctcgacgt
     2641 gcgctgctcg atcacgaccc gcttggccgg gccgctgcgg gcgcgccggc cgaaccccat
     2701 catgtccatc acggtcttga gctcgtagat gaacgcggcc atgtccttgt ggcgctcggc
     2761 gggtcgcttg gcgagcgcgt ggaagatcag ggtctcgagc gccggatcga cgccgccctc
     2821 gaccagctgc gacggcgacc gggccttctc gtcgaggtgg ccggcgagga tcttctggac
     2881 cgggccgtcc cacgggacct ggccggtgac cagctcgtag agcaggatgc cgacgccgta
     2941 gacgtcgctc gccggcgacg ccggctcgcc gcggatccgc tcgggcgcga cgtagtgcgg
     3001 ggtgcccgac agcgaggtcg agacccggct ggcgtggagc gagcgggcga gcccgaagtc
     3061 gagcagcttg atcacggtct tgttgcgctt gccctcgtgc tcctcctcgg agatcaggat
     3121 gttctcggtc ttgatgtcgc agtgcacgac gccctgcttg tggatgtagt gcagggcctc
     3181 ggcgacctgg agcacgatct cgcacgccct gcgggcggac agctgcttct cgcggaacag
     3241 gatccggttg agcgcctcgc cgtcgaccag ctccatcacc atgaacatcc cgaccttctc
     3301 gtcctcgccg aagtcgacca ccgtggtgat gttgggatgg gacatcgccg aggcgaaccg
     3361 cgcctcgcgg tagaacagct cgcgggcctc gtcggtctcg gcgacctggt tggagatgat
     3421 cttgagcgcg aacgtgcgcg acaggtggat gtgcttgacc ttgtagacct tgcccatccc
     3481 gccctggccg atccgggaca tgatctggta gcgcccgccg atcagcgacg agt
//
LOCUS       DASVOR010002209         3187 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_199291,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002209 DASVOR010000000
VERSION     DASVOR010002209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199291"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="VF516_40965"
     CDS             <1..703
                     /locus_tag="VF516_40965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8114192.1"
                     /translation="DVTGLRIGLPQEYFQAMDDDVRAAIARTASAMVERGAEIVDLSL
                     PHTELALPAYYLIAPAEASSNLARFDGVRYGQRAAAGDLTEMYARTRGAGFGPEVKRR
                     IMLGTYALRSGYYDAYYKKAQQIRTLIKRDFDKAFRYCDVILTPTTPTPAFAFGAKAT
                     PYEMYLADVFTLSCNLAGLPGISVPCGLSGDGLPIGAQLLGKPLDEATLLRAGQVIEA
                     AVGLGDRRPAIGGAA"
     gene            700..2256
                     /gene="gatB"
                     /locus_tag="VF516_40970"
     CDS             700..2256
                     /gene="gatB"
                     /locus_tag="VF516_40970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEX8114193.1"
                     /translation="MSQSRPGRQTQADGLAQGTPREPGNEIPGAGASGRDLPRVLEDW
                     EPVIGLEVHVQLATHSKIFSPSAVEFGAPPNSLTDPLVLGLPGTLPVFNQAVLQLALR
                     LGVATSSQIRARSRFARKHYFYPDLPKGYQISQFDEPLCENGHLDVILRGGLKRVRLQ
                     RIHLEEDAGKTSHVGAESHVDLNRAGVPLVEVVSAPELASAEEAGEFMRSLHRLVRWL
                     EISEGDMEKGQMRCDANVSVRRRGEDRLGTRTELKNINSFRFVEQAIEHEIARQIRTI
                     ETGEPIVRETRLWDADRGQSQPMRSKEEANDYRYFPDPDLPPLVVDGQIMAQTLRSLP
                     ELPSVRFDRYQKLGISADDARTLVSERSLADYFDAVLAAHPGDRSGRMLANWVLTELL
                     GALHASGTPVARSPMSPAALSALVVLVENGTISGKIGKDVFEESFRTGEAPGAIVERG
                     GLQQISDTAVLWEIINRIVAAHPKQAEAYTVGGKDGLFGFFMGQVMKETQGRANPVMT
                     SDLLHKRLGR"
     gene            2348..2668
                     /gene="rplU"
                     /locus_tag="VF516_40975"
     CDS             2348..2668
                     /gene="rplU"
                     /locus_tag="VF516_40975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEX8114194.1"
                     /translation="MYAVIQTGGKQYRVEPGKTVVVEKLAGDKGAPIVFDQVLLVSSG
                     DGGSVTIGKPTVSGAKVTGEIVEHGRGEKLVVFKFRRRKNYVRKNGHRQDYTAVKIAA
                     IEAA"
     gene            2705..2977
                     /gene="rpmA"
                     /locus_tag="VF516_40980"
     CDS             2705..2977
                     /gene="rpmA"
                     /locus_tag="VF516_40980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEX8114195.1"
                     /translation="MAHKKGQGSTRNGRDSLPKMRGVKRFAGEAVTAGSILVRQVGTK
                     FHAGENVGIGRDWTLFSKIDGVVKFERHGKFRMRVAVYPAAASAAA"
BASE COUNT          487 a         1166 c         1080 g          454 t
ORIGIN      
        1 cgacgtcacc ggcctgcgga tcgggctgcc ccaggagtac ttccaggcga tggacgacga
       61 cgtccgcgcc gcgatcgcgc gcaccgcgtc ggcgatggtc gagcgcggcg ccgagatcgt
      121 cgacctgtcg ctgccgcaca ccgagctcgc gctgccggcg tactacctga tcgccccggc
      181 cgaggcatcg tccaacctgg cgcggttcga cggcgtgcgc tacggccagc gcgcggcggc
      241 cggtgacctc accgagatgt acgcgcggac ccgcggcgcc gggttcggcc ccgaggtcaa
      301 gcgccggatc atgctcggca cctacgccct gcgctcgggc tactacgatg cctactacaa
      361 gaaggcccag cagatccgca cgctgatcaa gcgcgacttc gacaaggcat tccgctactg
      421 cgacgtgatc ctgaccccga ccacgccgac cccggcgttc gcgttcggcg ccaaggccac
      481 gccctacgag atgtacctgg ccgacgtgtt cacgctgtcg tgcaacctcg ccgggctgcc
      541 cggcatcagc gtcccgtgcg ggctgtccgg cgacggcttg ccgatcggcg cccagctgct
      601 cggcaagccg ctcgacgagg cgaccctgct ccgcgccggc caggtcatcg aggcggcggt
      661 cggcctcggc gatcgccgcc cggcgatcgg aggtgccgca tgagccagtc gcggccgggc
      721 cgccagacgc aggccgacgg gctcgcccag ggcacgccgc gcgagccggg caacgagatc
      781 cccggagccg gcgccagcgg gcgcgacctg ccgcgcgtgc tcgaggactg ggagccggtg
      841 atcggcctcg aggtccacgt ccagctcgcg acccacagca agatcttctc cccgtcggcg
      901 gtcgagttcg gcgcgccgcc caactcgctg accgatccgc tcgtgctcgg tctgcccggc
      961 acgctgccgg tgttcaacca ggcggtgctc cagctcgccc tgaggctcgg cgtcgccacg
     1021 tcgtcgcaga tccgcgcccg gtcgcggttc gcgcgcaagc actacttcta tcccgacctg
     1081 cccaagggct accagatctc gcagttcgac gagccgctgt gcgagaacgg ccacctcgac
     1141 gtgatcctgc gcggcgggct caagcgggtg aggctccagc gcatccacct cgaggaggac
     1201 gcgggcaaga cctcgcacgt cggcgccgag tcgcacgtcg atctcaaccg cgccggcgtg
     1261 ccgctggtcg aggtcgtgtc ggcgcccgag ctggcgtcgg ccgaggaggc cggcgagttc
     1321 atgcgctcgc tgcaccgcct ggtccgctgg ctcgagatct ccgagggcga catggagaag
     1381 ggccagatgc ggtgcgacgc caacgtgtcg gtccgccgcc gcggcgagga ccggctgggc
     1441 acccgcaccg agctcaagaa catcaactcg ttccggttcg tcgagcaggc gatcgagcac
     1501 gagatcgcgc gccagatccg gacgatcgag accggcgaac cgatcgtgcg tgagacccgg
     1561 ctgtgggacg ccgatcgcgg ccagagccag ccgatgcgca gcaaggaaga ggcgaacgac
     1621 tatcgctact tccccgatcc ggacctgccg ccgctcgtgg tcgacggcca gatcatggcc
     1681 cagaccctgc gctcgctccc cgagctgccg agcgtccggt tcgatcgcta ccagaagctc
     1741 gggatctcgg ccgacgatgc ccgcacgctg gtcagcgagc gctcgctcgc cgactacttc
     1801 gacgccgtgc tcgccgcgca cccgggcgat cgcagcggcc gcatgctcgc caactgggtg
     1861 ctcaccgagc tgctcggcgc gctgcacgcc agcggcaccc cggtcgcgcg ctcgccgatg
     1921 tcgccggccg cgctgtcggc gctggtcgtc ctcgtcgaga acggcacgat cagcggcaag
     1981 atcggcaagg acgtgttcga ggagagcttc cgcaccggcg aggccccggg ggcgatcgtc
     2041 gagcgcggcg gcctgcagca gatctccgac accgccgtcc tctgggagat catcaaccgg
     2101 atcgtcgccg cccaccccaa gcaggccgag gcctacaccg tgggcggcaa ggacggcctg
     2161 ttcgggttct tcatgggcca ggtgatgaag gaaacgcaag gccgtgccaa cccggtcatg
     2221 acctcggatc tattgcacaa acggctcggc cgctgagcct gccgcctcgc gcgcggcgcg
     2281 gttggcttga cgatggcccc gcgatctgta aagtccccgg ttccgaaggc cttttgcgga
     2341 gattgtcatg tacgccgtga tccagacggg tggtaagcag tatcgcgtcg agccgggcaa
     2401 gaccgtggtg gtcgagaagc tcgcaggcga caagggcgcc ccgatcgtgt tcgaccaggt
     2461 gctcctcgtg tcgtccggag acggcggcag cgtgaccatc ggcaagccga ccgtgagcgg
     2521 cgccaaggtc accggcgaga tcgtcgagca cgggcgcggc gagaagctcg tcgtgttcaa
     2581 gttccgccgc cgcaagaact acgtccgcaa gaacggtcac cgtcaggact acaccgcggt
     2641 caagatcgcc gcgatcgagg cggcctaggc cgcgctcatt gtacggagca cagaggtacg
     2701 cgtcatggca cacaaaaaag gacaaggttc gacccgcaac ggccgcgact cgctgccgaa
     2761 gatgcgcggc gtcaagcggt tcgccggcga ggcggtcacc gccggctcga tcctggttcg
     2821 ccaggtcggc accaagtttc acgccggcga gaacgtcggc atcggccgcg actggacgct
     2881 gttctccaag atcgacggcg tcgtgaagtt cgagcgccac ggcaagttcc ggatgcgcgt
     2941 cgcggtgtac cccgccgccg cctccgcggc tgcctagctc cgccccggcc gcgcgctctc
     3001 tcttctcggc tactcggccc acccgtccac ggcgccgggt cccgggccgc accctccacg
     3061 atgcgtgcac tgctcctggc cgtctcgctt gccgtcggcg ccgggctcgg cgcctgcgca
     3121 tcgtcgccgc caccgcgctt ccccgcgaag ccgcccggct gcgcgctgga gccggtcgag
     3181 cggctgc
//
LOCUS       DASVOR010002210         9034 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_285739,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002210 DASVOR010000000
VERSION     DASVOR010002210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9034
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285739"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VF516_40985"
     CDS             <1..257
                     /locus_tag="VF516_40985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114196.1"
                     /translation="RYALMGHRFDRGDVIDMLEESAIRHIPVVVQLRNGRVFEDRVTD
                     IGTWGEEDWVAFALHDFTPLRSISSCKRAWPPAYTYAGKR"
     gene            378..650
                     /locus_tag="VF516_40990"
     CDS             378..650
                     /locus_tag="VF516_40990"
                     /inference="COORDINATES: protein motif:HMM:NF016141.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEX8114197.1"
                     /translation="MLKKAIMDLLTWIIVGLIAGVLASLLVGSAGGIIMDIVIGVVGA
                     FVGGWIFHAAGWHTPFAGLAGTIFVAFVGAVVLLVVLHLIRGRPAV"
     gene            complement(701..1624)
                     /locus_tag="VF516_40995"
     CDS             complement(701..1624)
                     /locus_tag="VF516_40995"
                     /inference="COORDINATES: protein motif:HMM:NF024801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase C-terminal
                     domain-containing protein"
                     /protein_id="HEX8114198.1"
                     /translation="MGHLVDGTWHTGWYEPDAQGAFQRPAARFRNRPEEVVPGRYHLY
                     AAWACPWAHRTLITRSLRGLVGAISVTMVDPHMGDDGWVFRADDPDPIGGAQLLRDVY
                     LRADHGYTGRVTVPVLWDRRTATIVNNESREVMRIFDLDFAPLADRPLEPSLAPPELI
                     GRIDRVLDEIYAPINNGVYRAGFATSQAAYDVAVRELFAALAHWEGVLAEQPFVCGAQ
                     MTEADIALFTTLLRFDLVYHGHFKCNLKRLRDHPNLWRFTRRMYQHPGIRPTCKLDDI
                     KTHYYWSQPTVNPTRIVPIGPVLEPELSAPL"
     gene            1729..2061
                     /locus_tag="VF516_41000"
     CDS             1729..2061
                     /locus_tag="VF516_41000"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEX8114199.1"
                     /translation="MKCPGCANDMVQVEVQSIEIDRCPACGGIVLDKGESEAIELLGL
                     APVIEGGVSAEPGDGGRTEAARCHECNRDMIALRGAGDVEYDWCDGCERLFFDRGELT
                     AFEAFVDS"
     gene            complement(2133..2324)
                     /locus_tag="VF516_41005"
     CDS             complement(2133..2324)
                     /locus_tag="VF516_41005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114200.1"
                     /translation="MAAVLALVMLPIVAAVGCFIAIVRSDGSLSSAFAAATFIALAGF
                     VLVGALRIAREGEGRDIHH"
     gene            2478..3083
                     /locus_tag="VF516_41010"
     CDS             2478..3083
                     /locus_tag="VF516_41010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114201.1"
                     /translation="MRSLALVPCLLACGSSPPSPPPEPTARQALPWPVPDGFRGETIP
                     FPLDFAPGIAHKGVEELRFAPGFFDPAAPGYWSYTFVWRTEDAAAMDAAAVGGELTAY
                     FRGLIDAVDQARQITDRDAIAVRATPSGARLALAAHVFDAFKTKQPLDLVGWAERGAC
                     GTGALWRFVLAPANSALRPQLETLAAAAACDQPVPPPPPPR"
     gene            complement(3085..3564)
                     /locus_tag="VF516_41015"
     CDS             complement(3085..3564)
                     /locus_tag="VF516_41015"
                     /inference="COORDINATES: protein motif:HMM:NF019932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEX8114202.1"
                     /translation="MRLPALAFALAMFAACAASPPRVARFEPTDRAAITAVLDRQIAA
                     WNRGDLATYMEGYARTPALVFTSGGNIRRGWQEAFDHYHARYATDPKAMGTLAFQIDS
                     IEPVGADGAVVLGHWELTGTAHAGRGVFTLVVERRPEGWRIIHDHTSVSAEPAAPPS"
     gene            3651..4430
                     /locus_tag="VF516_41020"
     CDS             3651..4430
                     /locus_tag="VF516_41020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8114203.1"
                     /translation="MSTGRISLERRGHALVIGIDRVAKRNAFDLAMWDGLSRAYGELE
                     RDAELRVGVLYAHGDHFTAGLDLPQWAPTFARGGFAFPDGGLDPLGLRSRVTKPLVCA
                     VQGICLTLGIELLLAADIRIAARSTRFAQIEIKRGIYPVGGATLRFPREVGWANAMRW
                     LLTGDEFDAAEAHRIGLVQELTEHGEQLARALAIAEVIAEQAPLAVYATLAACRHALP
                     HSEAAATARLFPDLAPLVASDDMQEGLRAFVERRPGRFRGR"
     gene            4435..4845
                     /locus_tag="VF516_41025"
     CDS             4435..4845
                     /locus_tag="VF516_41025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114204.1"
                     /translation="MLDIALGRLFAQALVAIARADDEISPEEGLRLEQRIALRVAAPI
                     SLDDLLLAEPLEPHRLAEAVRGSSGPFRHVGVHPGELAAMIINDGIAVVIAKGYVSEA
                     EARVLVRFASALGCSRDEIRALCQRAAPWLGAMI"
     gene            complement(5365..7032)
                     /locus_tag="VF516_41030"
     CDS             complement(5365..7032)
                     /locus_tag="VF516_41030"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8114205.1"
                     /translation="MKTIGSFDYVIAGGGTAGCVLANRLSADPSVSVLMLEAGGRDKG
                     IWIHIPVGYLFCIGNPRTDWCYRIEPDPGLNGRTILYARGRVLGGCTSINAMLYMRGQ
                     ARDYDEWARLTGDPSWSWDRVLPVFKGTEDHWRGADDVHGAGGEWRVEQQRLSWEILD
                     AFRDAAAEVGIVKIEDFNRGDNAGSSRFEVNQRRGVRWNATKAFLRPVEHRKNLTVLT
                     GAPVHRLRLGRDAAGQPEAQGIEFYRGNDVVFAEARCETLLTAGSIGSPHILQFSGIG
                     PAAHLQAHGIPVVHDLPGVGGNLQDHLQLRTAFKVQNVLTLNTLANSWWGKVKMGLQY
                     AFRRTGPLTMAPSQMGACTRSDPDQPTANIEFHVQPLSLDKFGDPLHRYPAFTVSVCN
                     LRPTSRGHIRLKSPEPQAAPEIRLNYLSTPDDQRIAVQSIRLARRIINDTETMKKYAP
                     EEFVPGPSFQSDAELVTAAGNVGTTIFHPVGTCRMGRADDADAVVDPRLRVRGVARLR
                     VIDASIMPTITSGNTASPTVMIAEKGAAAVIEDRRRGRLLPASVDPS"
     gene            complement(7105..8040)
                     /locus_tag="VF516_41035"
     CDS             complement(7105..8040)
                     /locus_tag="VF516_41035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114206.1"
                     /translation="MKTALPCLLSLALATGACTDAPEPGPPADDPAEVTQAVTTTAFT
                     ASYTGQGSNATTCNTSFTITGQEPSTTGTFPVFLYMVGTSETATNASATAAVAAMASR
                     GFVAGTIAYASGAFGNCSQISGKARCIFNGASSTSALSVLCSRAKADCSKGVVVGGFS
                     QGAVIATLAKNFDSRVQAAWGMGDGVIYSIFNLTSCMGNGNRTLPSSRLRAVDGEKDQ
                     FIGPTQNNVRSQFLTLTGLSCGSTATNCLQSNGSGWYIVQNAQVQDGSADHCYMRRSG
                     DCGGSQNSLDANWQSGTDVWELGANLDWLKTFTTP"
     gene            complement(8441..>9034)
                     /locus_tag="VF516_41040"
     CDS             complement(8441..>9034)
                     /locus_tag="VF516_41040"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114207.1"
                     /translation="VAGDVTSAADRARIVAHVRERGPLHVLVHNAGSNVRAPLVNYDD
                     ATIEKLIALNLTSPVLLSRDLHPMLRAAGGASVIHVGSIAGQIALPTGVAYGASKAGI
                     AQVARTLALEWARDGIRVNTVAPWYTRTPLVEPVLSRPEMLAAILARTPLGRIAEPHE
                     VAAAVAFLALPAASYITGQCLAVDGGMSIQGLPAVIS"
BASE COUNT         1253 a         3090 c         3328 g         1363 t
ORIGIN      
        1 ggaggtacgc actcatggga catcgctttg atcgcggaga cgtgatcgac atgctcgagg
       61 agtccgcgat ccgtcacatc ccggtggtcg tgcagctgcg caacggccgg gtgttcgagg
      121 atcgcgtcac cgacatcggc acgtggggcg aggaggactg ggtggcgttt gcactccatg
      181 acttcacgcc gctgcgctcg atctcgagct gcaagcgcgc gtggccaccc gcgtacacgt
      241 acgcgggtaa gcggtagcgc ggcgcggccg ggcgatcgac cgggcgcctg accgggcgat
      301 cgggcgcatg cgcacagtcc gggcggcgcg cagcgagaca acctcgcgtc gcgccgaggg
      361 tcgcgcaggc acacgccgtg ctgaagaagg cgatcatgga cctcctcaca tggatcatcg
      421 tgggcctgat cgcaggcgtt ctcgcatcgc tcctggtcgg ctcggccggc ggcatcatca
      481 tggacatcgt gatcggcgtg gtcggcgcgt tcgtcggcgg ctggatcttc cacgccgcgg
      541 gctggcacac gccgttcgcc gggctcgccg gcacgatctt cgtcgcgttc gtcggcgcgg
      601 tggtgctgct cgtggtgctg cacctgatcc gcggtcgccc cgcggtgtga tcgccgtccg
      661 ccggagccct gacgacgccg gggcgtcggc gcgatcgccg ttataacgga gcggacagct
      721 cgggctcgag gaccgggccg atcgggacga tccgcgtcgg gttcacggtc ggctggctcc
      781 agtaatagtg ggtcttgatg tcgtcgagct tgcaggtcgg ccggatgccc gggtgctggt
      841 acatccgccg cgtgaaccgc cacaggttgg ggtgatcgcg cagccgcttc aggttgcact
      901 tgaagtggcc gtggtagacg aggtcgaacc ggagcagcgt ggtgaacagc gcgatgtcgg
      961 cctcggtcat ctgcgcgccg cacacgaacg gctgctcggc gagcacgccc tcccagtggg
     1021 ccagcgcggc gaacagctcg cgcaccgcga cgtcgtacgc ggcctggctg gtggcgaacc
     1081 cggcgcggta cacgccgttg ttgatcggcg catagatctc gtcgagcacg cggtcgatcc
     1141 ggccgatcag ctcgggcggc gccagcgacg gctcgagcgg gcgatcggcg agcggcgcga
     1201 agtcgaggtc gaagatccgc atgacctcgc gcgactcgtt gttgacgatc gtcgcggtgc
     1261 ggcgatccca cagcaccggc acggtgaccc ggccggtgta accgtggtcc gcgcgcaggt
     1321 aaacgtcgcg caatagttgt gcgccgccga tcggatccgg gtcgtcggcc cggaacaccc
     1381 agccgtcatc gcccatgtgc gggtcgacca tcgtgacgct gatcgcaccg accagcccgc
     1441 gcagcgagcg cgtgatcagc gtccggtgcg cccacgggca ggcccacgcc gcgtacagat
     1501 ggtagcggcc ggggacgacc tcctcgggcc ggttgcgaaa ccgcgcggcc ggacgctgga
     1561 acgcgccctg ggcatcgggc tcgtaccacc ccgtgtgcca ggtgccgtcc acgaggtgtc
     1621 ccatgctgga tcgctaccat tcctcgcggg cgggtgaaac tgtcacatca tgcggaggag
     1681 tgcaattgtc tgtttcgcga tttgaggact ggggtactgt cggcggcgat gaagtgtccc
     1741 ggctgcgcga acgatatggt gcaggtcgag gtgcaatcga tcgagatcga tcgctgcccg
     1801 gcgtgtggcg gcatcgtgct cgacaagggc gagagtgagg cgatcgagtt gctgggcctt
     1861 gccccggtga tcgagggcgg ggtgtcggcc gagccgggcg acggcgggcg tacggaggcg
     1921 gcgcgctgtc acgagtgcaa ccgcgacatg atcgcgctgc gcggggcggg cgacgtcgag
     1981 tacgactggt gcgacggctg cgagcggctg ttcttcgacc gcggcgagct gaccgcgttc
     2041 gaggcgttcg tcgacagcta gggaatcctc gcgggtaccc cggctgcctg gaacgcagcg
     2101 ttgcgccgcc ggcctgatcg gcgtgcgccg agctagtggt ggatgtcgcg gccctcgccc
     2161 tcgcgggcga tccgcagcgc gccgaccagg acgaagccgg cgagcgcgat gaaggtcgcg
     2221 gcggcgaacg ccgaggacag cgagccgtcg gatcggacga tggcgatgaa gcatccgacg
     2281 gcggcgacga tgggaagcat gacgagggcg aggacggcgg ccatggctgc ccgttgtgcg
     2341 cgggatatgc cagtcgcgcg gtcgccaacc ctgcgcgatc gcgaggcaaa cgcgcaaacc
     2401 gtgcgagcgg gcggccggcg gcgcgaatcg gttgcggaat tggcggccgc ggcggcgggc
     2461 gtgcgaaggt cgagccgatg cgcagcctgg ccctggtccc ttgcctcctc gcgtgcggtt
     2521 cgtcgcctcc gtcgccgcca cccgagccga ccgcgcggca ggcgttgccg tggccggtgc
     2581 ccgacgggtt tcgcggcgag accatcccgt tcccgctcga cttcgcgccc ggcatcgcgc
     2641 acaagggcgt ggaagagctg aggtttgcgc cggggttctt cgatccggcg gcgccgggct
     2701 actggtcgta caccttcgtg tggcggaccg aggacgcggc ggcgatggac gccgcggcgg
     2761 tcggcggcga gctgaccgcg tacttccgcg gcctgatcga cgcggtcgac caggccaggc
     2821 agatcaccga ccgcgacgcg atcgccgtgc gcgcgacccc gtccggcgcc cggctcgcgc
     2881 tggccgccca cgtgttcgac gcgttcaaga ccaagcagcc gctcgacctc gtcgggtggg
     2941 cggagcgcgg ggcgtgtggg accggcgcgc tgtggcggtt cgtgctggcc ccggcgaaca
     3001 gcgcgctccg gccgcagctc gagaccctcg cggcggccgc ggcatgcgat cagccggtgc
     3061 cgccaccccc gccgccgcgg tgagtcagct cgggggcgcc gcgggctccg ccgaaacgga
     3121 cgtatggtcg tggatgatcc gccagccctc gggccggcgc tcgacgacca gcgtgaacac
     3181 gccgcgcccg gcgtgagccg tgccggtgag ctcccagtgg ccgagcacca ccgcgccgtc
     3241 ggcgccgacc ggttcgatcg agtcgatctg gaacgccagc gtgcccatcg ccttcggatc
     3301 ggtcgcgtac cgcgcgtggt agtgatcgaa cgcctcctgc cagccgcgcc ggatgttgcc
     3361 gccggacgtg aacaccagcg cgggcgtgcg ggcgtagccc tccatgtagg tcgcgaggtc
     3421 gccgcggttc cacgccgcga tctggcgatc gagcacggcg gtgatcgcgg cgcgatcggt
     3481 gggttcgaac cgcgcgacgc gcggcgggct ggccgcacag gcggcgaaca tcgcaagggc
     3541 gaacgcgagc gcggggagcc gcatgccgcg agcctaccgc gggccggcgg tgcggcgcat
     3601 cacggccggc gcgatggcgc gcgccccgcg ggatgccgta ccttcggatc atgtcgactg
     3661 gacggatcag cctggagcgc cgcggccacg cgctggtcat cggcatcgac cgggtggcca
     3721 agcgcaacgc gttcgatctc gcgatgtggg acgggctgtc ccgggcgtac ggcgagctcg
     3781 agcgcgacgc cgagctgcgg gtcggcgtgc tgtacgcgca cggcgatcac ttcaccgcgg
     3841 ggctcgacct gccgcagtgg gcgccgacgt tcgcgcgcgg cgggttcgct ttccccgacg
     3901 gcgggctcga tccgctcggc ctgcggtcgc gggtgaccaa gccgctcgtg tgcgcggtcc
     3961 agggcatctg cctgacgctg ggcatcgagc tcctgctcgc ggccgatatc cggatcgccg
     4021 cgcggtcgac ccggttcgcg cagatcgaga tcaagcgcgg gatctacccg gtcggcggcg
     4081 cgacgctgcg gttcccgcgc gaggtcgggt gggccaacgc gatgcgctgg ctgctcaccg
     4141 gcgacgagtt cgacgccgcg gaggcccacc ggatcggcct ggtccaggag ctcaccgagc
     4201 acggcgagca gctggcgcgc gcgctcgcga tcgccgaggt catcgccgag caggcgccgc
     4261 tcgcggtcta cgcgacgctg gcggcgtgcc gccatgcgct gccgcacagc gaggcggcag
     4321 ccacggcgcg gctgttcccg gatctcgcgc cgctggtcgc cagcgacgac atgcaggagg
     4381 gcctgcgcgc gtttgtcgag cgccgcccgg gccggttccg tggccgctga cccgatgctc
     4441 gacatcgcgc tcggccgcct gttcgcccag gcgctggtcg ccatcgcgcg ggccgacgac
     4501 gagatcagcc ccgaggaggg cctgcggctg gagcagcgga tcgcgctgcg ggtcgccgcg
     4561 ccgatctcgc tcgacgacct gctgctcgcc gagccgctcg agccgcaccg gctcgccgag
     4621 gcggtgcgcg gcagctcggg gccgttccgc cacgtcgggg tccaccccgg cgagctcgcc
     4681 gcgatgatca tcaacgacgg gatcgccgtc gtgatcgcca agggctacgt gtcggaggcc
     4741 gaggcccgcg tgctggtccg gttcgcctcg gcgctcggct gctcgcgcga cgagatccgc
     4801 gcgctgtgcc agcgggcggc gccctggctg ggcgcgatga tctgatcggg gcctcagctc
     4861 agcacgcgaa ctcgcagaag ctcacgaggt cctcgtacga gggaccgcag atgccagcat
     4921 cgaacgtggc cgtcggactc gtgcagaagc agtttgcaca aacggtctcg ccgtcgttcc
     4981 ccccgggctc tggctcgccc agctcgaagg atacgccgca tgcgtttgac ccattgcaca
     5041 cgtcgccgta gcggtagcac atgggaatct cttgaccgcc atcacaaaac catccggtcg
     5101 ggcagtcgtc gttcgacata catccccagg tatccgcctg ggacgtttgt gcaacggtgt
     5161 cctcggacat ggtggaacag gccatcagca tccaccccat ggctccggca atcaggagct
     5221 ggaccaccct ctgcgtctgc cgctcgtgac acttcatcgc atcctccgtc cgtgctcccg
     5281 agtccggggg agcgtcaccc gctccaccag gttcgcacgg gaaacagagt ttgcggagag
     5341 ttctgctgga caatggctac aaacctacga cgggtcgacc gatgccggta gcagccgacc
     5401 gcgccggcgg tcctcgatca cggccgcggc gcccttctcg gcgatcatca cggtgggcga
     5461 cgcggtgttg cccgaggtga tcgtgggcat gatcgaggcg tcgatgacgc gcagccgcgc
     5521 cacaccgcgc acccggagcc gggggtcgac cacggcgtca gcgtcgtcgg cgcgccccat
     5581 ccggcaggtg ccgaccgggt ggaagatcgt cgtgcccacg ttgccggccg cggtcaccag
     5641 ctcggcgtcg gactggaacg acggccccgg gacgaactct tccggcgcgt acttcttcat
     5701 ggtctcggtg tcgttgatga tgcggcgcgc caggcggatc gactgcacgg cgatccgctg
     5761 gtcgtcgggc gtggacaggt agttgagccg gatctccggt gcggcctgcg gctcgggcga
     5821 cttgaggcgg atgtggccgc gcgacgtcgg gcgcaggtta cacacgctca ccgtgaacgc
     5881 gggataccgg tgcagcggat cgccaaactt gtcgagcgac agcggctgga cgtggaactc
     5941 gatgttcgcg gtcggctgat cggggtcgga cctcgtgcac gcgcccatct gcgatggcgc
     6001 catggtgagc ggcccggtgc gccggaacgc gtactggagg cccatcttca ccttgcccca
     6061 ccacgagttg gcgagcgtgt tgagcgtgag cacgttctgc accttgaacg cggtgcggag
     6121 ctgcaggtgg tcctgcaggt tgccgcccac gccggggagg tcgtggacca cggggatgcc
     6181 gtgcgcctgg aggtgcgcgg ccggaccgat gcccgagaac tgcaggatgt ggggcgagcc
     6241 gatcgatccc gcggtcagca gggtctcgca gcgcgcctcg gcgaacacca cgtcgttgcc
     6301 gcggtagaac tcgatgccct gggcctcggg ctggccggcc gcgtcgcgcc cgagccgcag
     6361 gcggtggacc ggcgcgccgg tgagcacggt gaggttcttg cggtgctcga ccggacgcag
     6421 gaacgccttg gtcgcgttcc accgcacgcc gcggcgctgg ttgacctcga accgcgagct
     6481 gcccgcgttg tcgccgcggt tgaagtcctc gatcttgacg atgccgacct cggcggcggc
     6541 gtcgcggaac gcgtcgagga tctcccacga cagccgctgc tgctcgaccc gccactcgcc
     6601 gccggcgccg tggacgtcgt cggcgccgcg ccagtggtcc tcggtgccct tgaacaccgg
     6661 gagcacgcgg tcccacgacc agctcggatc gccggtcagg cgggcccact cgtcgtagtc
     6721 gcgggcctgg ccgcgcatgt acagcatcgc gttgatcgag gtgcagccgc ccagcacgcg
     6781 gccgcgcgcg tagaggatcg tgcggccgtt gagcccgggg tcgggctcga tgcggtagca
     6841 ccagtcggtg cgcgggttgc cgatgcagaa caggtagccg acggggatgt ggatccagat
     6901 ccccttgtcc cggccgccgg cctccagcat cagcaccgag accgacggat cggcggacag
     6961 ccggttggcg agcacgcagc cggcggtgcc gccgccggcg atcacgtagt cgaacgagcc
     7021 gatggtcttc atgggtccct ccgggcgcct ctggcctggc gccgcaggat atcgggaccc
     7081 gccggcccgg tcagcccgcg ccggtcacgg cgtggtgaac gtcttcagcc agtcgaggtt
     7141 ggcgcccagc tcccagacgt cggtgccgct ctgccagttg gcgtcgagcg agttctggct
     7201 gccgccgcag tcgccgctgc gccgcatgta gcagtggtcg gcgctgccgt cctggacctg
     7261 cgcgttctgc acgatgtacc agccgctgcc gttggactgc aggcagttgg tcgcggtgct
     7321 gccgcacgac agcccggtca gggtcaggaa ctggctgcgc acgttgttct gggtggggcc
     7381 gatgaactga tccttctcgc cgtcgaccgc gcgcagccgg ctgctcggca gcgtgcggtt
     7441 gccgttgccc atgcacgagg tcaggttgaa gatcgagtag atcacgccgt cgcccatgcc
     7501 ccacgcggcc tggacccggc tgtcgaagtt cttggccagc gtcgcgatca ccgcgccctg
     7561 gctgaacccg ccgaccacca cgcccttgct gcagtcggcc ttggcgcgcg agcacagcac
     7621 cgacagcgcg ctggtcgagc tcgcgccgtt gaagatgcag cgcgccttgc ccgagatctg
     7681 gctgcagttg ccgaacgccc cgctcgcgta cgcgatggtc ccggcgacga acccgcggct
     7741 cgccatggcg gcgaccgccg ccgtcgccga cgcgttggtc gcggtctcgc tcgtgccgac
     7801 catgtagagg aacaccggga acgtgccggt ggtcgacggc tcctgcccgg tgatggtgaa
     7861 gctggtgttg cacgtcgtcg cgttgctgcc ctggccggta taggacgcgg tgaaggcggt
     7921 cgtggtgacc gcctgggtga cctcggcggg gtcgtcggcc ggcgggccgg gctcgggcgc
     7981 gtcggtgcac gcgccggtgg cgagggcgag cgagagcaag cagggtagcg cagtcttcat
     8041 gtccggttct cctgtcgcga ggcgttgccg gcgggcacga ccgtccagct ctcccatgcg
     8101 cgcgtcagcg agccatcagc cagatgagac gcgatggttc ctgaaccgct ccagcgtcgt
     8161 gatgccgtta tgccgcacgc tgccacaggg acgagcaccg agtaaagtac ccgcctcgcg
     8221 gcacccacat taaggaacgg atcagttcct gtatagcaag tgtcgcggca tacaaccgcg
     8281 cggatgcggt gggagacctg ccgccaacgt cccagctatg cacggccgca gcgcaccgca
     8341 gcaggccggg cccgcagcgg atcgctgcgc tggcctggcg gtccgcggcg gatcgcgcgc
     8401 gatccgatcg ccgtgacgtt tcgggggacg gatcccaccg ctacgagatc acggccggca
     8461 ggccctggat cgacatcccg ccgtcgaccg cgaggcactg cccggtgatg tacgacgccg
     8521 ccggcagcgc gaggaacgcg accgccgctg ccacctcgtg gggctcggcg atccggccga
     8581 gcggcgtgcg cgcgaggatc gcggcgagca tctcgggccg cgacagcacc ggctcgacca
     8641 gcggcgtgcg cgtgtaccac ggcgccacgg tgttgacccg gatcccgtcg cgcgcccact
     8701 cgagcgccag cgtgcgcgcg acctgcgcga tcccggcctt ggacgcgccg taggccactc
     8761 ccgtcggcag cgcgatctgc ccggcgatcg agccgacgtg gatcacgctc gcgcccccgg
     8821 cggcgcgcag catcgggtgg aggtcgcgcg acagcagcac cggcgacgtg aggttgagcg
     8881 cgatcagctt ctcgatcgtc gcgtcgtcgt agttgaccag cggggcgcgc acgttgctgc
     8941 cggcgttgtg gaccagcacg tggagcggcc cgcgctcgcg gacgtgggcg acgatccgcg
     9001 cgcgatccgc ggcgctggtc acgtcgccgg ccac
//
LOCUS       DASVOR010002211         1829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_285752,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002211 DASVOR010000000
VERSION     DASVOR010002211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285752"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VF516_41045"
     CDS             <1..251
                     /locus_tag="VF516_41045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114208.1"
                     /translation="RCRVGTLIAGSRGWGCARWREGCRFVVWFETAGRRLTPAQLRDL
                     VIRGKTRKARFRSDHGAEIDGRLVLDPSADGGARLEPA"
     gene            837..>1829
                     /locus_tag="VF516_41050"
     CDS             837..>1829
                     /locus_tag="VF516_41050"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114209.1"
                     /translation="METGSQIGPFVVLRLLGKGGMGSVYLAEHTLMKDLHAIKILDPQ
                     LTQNPAIVTRFVNEARAAARLRHRNLVRVHNIERLPDDGSWFMVLDYLDGRTLARFMA
                     AQRGPMPPTTILHILAQVANCIEHVHDHKIVHRDLKPDNIFLIQHGDDPHFPVVLDLG
                     VAQLSEDLAAGPATKTGTVIGTPIYMAPEQLRGERVSPAADIFALGVIAYEMSTGGFF
                     PYQYDEPRSAYFELPPTEIYYRQRSSPPVDPRRRCPGLGDAWVDILRAALDPDPRVRP
                     PSAGAFARMLAAAVPASQGDGHAILHQVARELTQHRGSDGDLRGSGPAAVLRDAG"
BASE COUNT          280 a          661 c          588 g          300 t
ORIGIN      
        1 cgcgctgccg cgtcggcacg ctgatcgccg gcagtcgcgg ctggggctgc gcgcgctggc
       61 gcgagggctg tcgcttcgtc gtctggttcg agaccgccgg ccgccggctc acgcccgcgc
      121 agctccgcga cctggtcatt cgcggcaaga cccggaaggc gcggtttcgc tccgatcatg
      181 gcgcggagat cgacggccgc ctcgtcctcg atccgtcggc cgacggcggc gctcggctcg
      241 agccggcgtg atcgcctgcg ccgcgatcca cctgccagca ggaccgtgca ccgctccaca
      301 cttccctacg ctggtggccg ggcaagcagt gcgaccgtct ccggtcgaag cttctggcgt
      361 tcacagcggc tcgaagcgtt ctgcatgtgc gtacgctaca gccatgctgg atcctgtatg
      421 gctcacgacg tggcccccac acctcgccca catcgcgcat gtcgtagtcg aacgagcgta
      481 cggcctgggc gcagcatctc gctgcagcgg caccacaaca tcacctgcag cggcgggtgt
      541 gccgcggatt gccggtttgt tctcgcagct tcggatatcg agtatggatc aatgcttgat
      601 cggtcgaaac cgataccgcc aagatcctct gtaccgggga ctacccgatt cccagggtgc
      661 gattgctgcc ttccaggcac gccacgcgtg agcatttgtc cgtgcggtgc gccacatgct
      721 gcggcggcgt gcagcgcatc gtggcacggc caagtgtccg cataaccttg atattcctgg
      781 cgtcgtaagc gaacttcctg ctcgcagctc gggtcagccg ggcgcgggct tgggacatgg
      841 agacaggcag ccagatcggg ccattcgtgg tgttgcgact gctgggcaag ggcggaatgg
      901 ggagtgttta tttggcggaa cacactctca tgaaggatct gcacgcgatc aagatcctcg
      961 acccgcagct gacccagaat cccgcgatcg tcacccggtt cgtcaacgag gcgcgcgccg
     1021 ccgcccggct ccgccaccgc aacctggtcc gcgtccacaa catcgagcgg ctgcccgacg
     1081 acgggtcgtg gttcatggtg ctcgactacc tcgacggccg cacgctcgcg cggttcatgg
     1141 cggcgcagcg cgggccgatg ccgccgacca cgatcctgca catcctcgcg caggtcgcca
     1201 actgcatcga gcacgtccac gaccacaaga tcgtccaccg cgatctcaag cccgacaaca
     1261 tcttcctgat ccagcacggc gatgatccgc attttccggt cgtgctcgac ctcggcgtcg
     1321 cgcagctgag cgaggacctg gcggccgggc cggcgaccaa gaccgggacg gtgatcggga
     1381 ccccgatcta catggcgccg gagcaactcc gcggtgagcg ggtctcgccg gcggctgaca
     1441 tcttcgcgct cggcgtcatc gcatacgaga tgagcaccgg cggattcttc ccgtaccagt
     1501 acgacgagcc ccgatctgcc tacttcgagc tcccgccgac cgagatctac taccgccagc
     1561 gcagcagccc gccggtcgac ccgcggcggc gatgccccgg actcggcgat gcctgggtcg
     1621 atatcctgcg cgctgcgctc gatcccgacc cgcgcgtgcg accgccgagc gccggggcat
     1681 tcgcccgcat gctcgccgcc gcggtgccgg ccagccaggg cgacggccac gcgatcctcc
     1741 accaggtcgc gcgcgagctg acccagcacc gcggctccga cggcgatctt cgcggctcgg
     1801 gtccagccgc ggtgctccgc gacgccggc
//
LOCUS       DASVOR010002212         1541 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_402922,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002212 DASVOR010000000
VERSION     DASVOR010002212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402922"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VF516_41055"
     CDS             <1..249
                     /locus_tag="VF516_41055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114210.1"
                     /translation="QLVTQHGTLGQATPRAIREERREFARGALLVLCSDGIRSRWSLD
                     GHPGLAGRDPVTIATVLWRDFARGRDDASLVVVREAAR"
     gene            246..>1541
                     /locus_tag="VF516_41060"
     CDS             246..>1541
                     /locus_tag="VF516_41060"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8114211.1"
                     /translation="MTELGLVDVRGDDDVVRAHQTTRTIAAQLGFSSFEQTRIATAVS
                     EIARNALTYAAGGTVTFGLDDAGGALAITVVDRGPGIPDVAALLSGERAQTAGLGAGI
                     AGARRLMDTFVIDSSPGGTRVEMTRRIPRRTGKPDAARVTTMQLQLAGQLADDPSQEI
                     RRLQRDIAERDRRLVELTEELTETNRGVMALHAELEDRAEYQRRAVELRTRLLSEMGH
                     ELRTPLHSLATISQFLLDRLDGELTSEQDRQVRIIHEVAESLTTYVNDLLDLARTDAG
                     RAVVHLTRFPVDAVLRPLRRILQPLVPPGVALRVAVDPGLPPITSDEQKLSQILRNLV
                     ANALKFTARGAVEIRAQRAGDRVVFEVADTGIGIAPEHHELIFHEFAQVDGHVQPRMR
                     GSGLGLPLSRRLTELLGGTLTVASELGVGSTFRVELPLEA"
BASE COUNT          202 a          583 c          563 g          193 t
ORIGIN      
        1 cagctggtca cccagcacgg cacgctcggc caggccacgc cgcgcgcgat ccgcgaggag
       61 cgccgggagt tcgcgcgcgg ggcgttgctc gtgctgtgca gcgacggcat ccgatcgcgg
      121 tggagcctcg acggccaccc cgggctcgcc ggccgcgatc cggtgacgat cgcgaccgtg
      181 ctgtggcgcg acttcgcgcg cggccgcgac gacgcctcgc tcgtggtggt gcgagaggcc
      241 gcgcgatgac cgagctcggt ctggtcgacg tgcgcggcga cgacgacgtc gtgcgcgctc
      301 accagaccac gcggacgatc gcggcgcagc tcgggttctc gagcttcgag cagacccgga
      361 tcgcgaccgc ggtgtccgag atcgcgcgca acgcgctgac ctacgcggcg ggcggcacgg
      421 tgacgttcgg gctcgacgac gccggcggcg cgctggcgat cacggtggtc gaccgcggcc
      481 ccgggatccc cgacgtcgcg gcgctcctgt ccggcgagcg ggcgcagacc gcggggctgg
      541 gcgccggcat cgcgggcgcg cggcggctga tggacacgtt cgtgatcgac tcgtcgcccg
      601 gcggcacccg ggtcgagatg acccggcgca tcccgcggcg caccggcaag cccgacgcgg
      661 cgcgggtcac cacgatgcag ctccagctcg ccggccagct cgccgacgat ccgagccagg
      721 agatccgccg gctgcagcgc gacatcgccg agcgcgatcg ccggctggtc gagctgaccg
      781 aggagctgac cgagaccaac cgcggcgtga tggcgctgca cgccgagctc gaggatcgcg
      841 ccgagtacca gcgccgcgcg gtcgagctcc gcacccgcct gttgtcggag atgggccacg
      901 agctgcgcac gccgctgcac tcgctggcga cgatctcgca gttcctgctc gaccggctcg
      961 acggcgagct cacctccgag caggaccgcc aggtccggat catccacgag gtcgccgagt
     1021 cgctcaccac gtacgtcaac gacctgctcg acctggcgcg caccgacgcc ggccgcgcgg
     1081 tcgtccacct cacgcggttc ccggtcgacg ccgtgctgcg gccgctgcgc cggatcctcc
     1141 agccgctggt cccgccgggg gtcgcgctgc gggtcgcggt cgaccccggg ctgccgccga
     1201 tcaccagcga cgagcagaag ctgtcgcaga tcctccgcaa cctggtcgcc aacgcgctca
     1261 agttcaccgc gcgcggcgcg gtcgagatcc gcgcgcaacg cgccggcgat cgcgtcgtgt
     1321 tcgaggtcgc ggacacgggg atcggcatcg cgcccgagca ccacgagctg atcttccacg
     1381 agttcgcgca ggtcgacggc cacgtccagc cccggatgcg cggcagcggc ctgggcctgc
     1441 cgctgtcgcg ccggctcacc gagctgctcg gcggcacgct cacggtcgcc agcgagctcg
     1501 gcgtcggctc gacgttccgg gtcgagctgc cgctggaggc c
//
LOCUS       DASVOR010002213         1633 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_169864,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002213 DASVOR010000000
VERSION     DASVOR010002213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1633
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169864"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="VF516_41065"
     CDS             complement(<1..871)
                     /locus_tag="VF516_41065"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEX8114212.1"
                     /translation="MRVQVTVVAALVGGACGDNTLPVGEPLSAAVHLTIVAHPDDDLL
                     FLQPDLQDALNGGGGMTSVYVTAGNDTHGLSYVEDRYAGLMAAYGAIAGTHDWICGWI
                     DIADHAAEHCRLDEAGISLVFLGYPDGGLMGERPSSLRNLWQGHVASATTISRRPATY
                     ERADLIAVLSEIVTATAPITLRTLEVSATHGHDHSDHMVVGALAVVATAATPERPELI
                     SYRGYNIEREPANADPALFERARDIMARYQSCTTHCAPCGQPCATHHLSPADLGWLHR
                     RYAVGMRRRGEGQL"
     gene            1081..>1633
                     /locus_tag="VF516_41070"
     CDS             1081..>1633
                     /locus_tag="VF516_41070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114213.1"
                     /translation="MLAWLRSAGRGLASSPAAQLVVGAAILALAFPGRVTGSLGLAVL
                     AGALGIAVRVVWPRLPDFVRAPPDSTATAVVIAIVAVAGVAVFGDAMTGSPDWQMGDW
                     GPQRAVLSHVMPALPGLDLPVWNQVVSTGDAPLELYPSLTYLVTGHVALALGLTHDLP
                     LALMVVAVIVHLAIAVATAAIAIQ"
BASE COUNT          191 a          569 c          625 g          248 t
ORIGIN      
        1 ggagctggcc ctcgccgcgc cgccgcatgc cgaccgcgta gcgccggtgc agccagccga
       61 ggtccgccgg gctgaggtgg tgggtcgcac agggctggcc gcacggcgcg cagtgggtgg
      121 tgcagctctg gtagcgcgcc atgatgtcgc gcgcgcgctc gaacagcgcg gggtcggcgt
      181 tggccggctc gcgctcgatg ttgtagccgc ggtacgagat cagctcgggg cgctccgggg
      241 tcgcggcggt cgccaccacc gcgagcgccc cgaccaccat gtggtcggag tgatcgtggc
      301 cgtgggtcgc ggagacctcg agggtgcgca gcgtgatcgg cgcggtggcg gtgacgatct
      361 cggacagcac cgcgatcagg tcggcgcgct cgtaggtcgc ggggcgccgg gagatcgtgg
      421 tcgcggacgc gacgtgacct tgccacaggt tgcgcaggct gctcggccgc tcgcccatga
      481 gcccgccgtc ggggtagccc aggaacacca gcgagatccc cgcctcgtcg agccggcagt
      541 gctcggcggc gtgatccgcg atgtcgatcc agccgcagat ccagtcatgg gtgccggcga
      601 tcgctccgta cgccgccatc aggcccgcgt agcggtcctc gacgtagctc agcccgtggg
      661 tgtcgttgcc ggcggtgacg tagacgctgg tcatgccgcc cccgccgttg agcgcgtcct
      721 gcaggtcggg ctgcaggaac agcagatcgt cgtcggggtg agcgacgatc gtcaggtgga
      781 ccgccgccga cagcggctcg ccgaccggca gtgtattgtc gccacaggcg cctccgacca
      841 gagctgccac cacggtcacc tggacgcgca ttgcggccag tgtgcgctga tctcgcatcc
      901 cgccgtcaag cgcgcttttt taagagaatg tggcagctgt gctacagaat gtttcctgcg
      961 cgccacggat cgggccgctg cccgagcgcc cctgcccgca ccccggcgcg cggctgagcg
     1021 ggagcgccgg ggcttacccg acgttgtgcg cgccgcggcg gccgtgctaa cgcggcgaac
     1081 gtgctcgcat ggctgcgatc ggcagggcgg gggctcgcgt cgagcccggc ggcgcagctc
     1141 gtggtcggcg cggcgatcct ggccctggcg ttccccggtc gggtcaccgg gtcgctcggc
     1201 ctggccgtgc tcgccggcgc gctcgggatc gccgtgcggg tggtctggcc gcggctgccg
     1261 gacttcgtgc gggcgccccc cgacagcacc gcgaccgcgg tggtgatcgc gatcgtcgcg
     1321 gtggccgggg tcgcggtgtt cggcgacgcg atgaccggat cgccggactg gcagatgggc
     1381 gactgggggc cgcagcgcgc ggtgctgagc cacgtgatgc cggcgctgcc cggcctcgac
     1441 ctgccggtgt ggaaccaggt ggtgtcgacc ggcgacgcgc cgctcgagct ctacccgtcg
     1501 ctcacctacc tggtcaccgg ccacgtcgcg ctcgcgctcg gcctgaccca cgacctgccg
     1561 ctcgcgctga tggtcgtcgc ggtgatcgtc cacctggcga tcgcggtggc gaccgcggcg
     1621 atcgcgatcc agg
//
LOCUS       DASVOR010002214         2302 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_228528,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002214 DASVOR010000000
VERSION     DASVOR010002214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228528"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            490..1158
                     /locus_tag="VF516_41075"
     CDS             490..1158
                     /locus_tag="VF516_41075"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEX8114214.1"
                     /translation="MTRQAVAGFAFLFAALAAALFGPAWTLDYWQAWVFLAVFFLAAA
                     AITVDLARRDPALLARRVQVGPTAEPTPRQKWIQAIASLAYLAVFVVAGLDHRAGWSR
                     VPVATVVAGDALVVLGMFVVFRVFRANTFTSAVIDVDRDQQLVSSGPYAVVRHPMYAG
                     ALVMMAGVPIALGSWWALVPVVAMTAVIVARLLDEEALLAARLPGYAEYRSRVQQRLV
                     PRVW"
     gene            1178..2029
                     /locus_tag="VF516_41080"
     CDS             1178..2029
                     /locus_tag="VF516_41080"
                     /inference="COORDINATES: protein motif:HMM:NF014623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HEX8114215.1"
                     /translation="MDALRDVAPRPEASTESTRDSMRDSIRDLGARFEARAVNVAERI
                     DVRGIDPRLSNHLPVTVAVGSGCAMLFRSGAVVFFAVDTVAQERFQHDLGPRIHGPYQ
                     VPESERATIRIGDADAVEPDALVIKESSIERMQVIAEILAKSVVLARNEQEIGTAFDA
                     IEPLAAEMKRAPRKLPWRHSDLVRHIGEAMLVEHQLVDRAEVLEKPELLWDRADLDRL
                     YARLEDEYEIRERHLVLESKLSLVTTAARTMLELNQARRSLNVEYYILALILVEVVLA
                     LYQIFRL"
     gene            complement(2038..>2302)
                     /locus_tag="VF516_41085"
     CDS             complement(2038..>2302)
                     /locus_tag="VF516_41085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114216.1"
                     /translation="ASHAFALQERGLAVIAEQVARALRPEPDPALLRDVEITLLTAVS
                     AAYGYALAKHTLAPALGREPSRELDDEVRHTLAAMVQTQLRPR"
BASE COUNT          293 a          811 c          862 g          336 t
ORIGIN      
        1 ccccgccgcg cgcgccggat cgaacccgcg cccggcgacc gcgccatccc cgcggcggaa
       61 ccgctggttg ccttcgacga ggcgctccag cgcggcctcg ggcgacagcg acagcgggtc
      121 ccaggtgtgc ggcatgccgc actcatacgc gaagcccggg ccgaacacca gcgccgcggt
      181 gccctcgcag cgatggccgc gatctcgcgt gtgcgcgggc gctcggatcc ctcggatagg
      241 gagcaccgcg agctcgcgct ccccaggccg cgccgggtcg ccaggatggc gctctcgatg
      301 acccggtcgt cgtcgatcgg gaagcgcagc acgcggagca gccgcccgcg ccgcgccagg
      361 accaggccat cgcgcggatc aacgccttga accgggtcaa cgtcacgagc cgccagatcg
      421 cgctcggccc gcgcggatcc gcggcgagcc ggtgcgggcc gcgcgtggta ctacaggatc
      481 atgacacgcg tgacccgcca agctgtcgcg ggcttcgcct tcctgttcgc ggcgctcgcc
      541 gccgcgctgt tcggaccggc gtggacgctc gattactggc aggcgtgggt gttcctcgcg
      601 gtgttcttcc tcgcggcggc cgcgatcacg gtcgacctgg cgcgacgcga tccggcgctg
      661 ctggccaggc gcgtgcaggt cggcccgacc gccgagccca cgccgcgcca gaagtggatc
      721 caggcgatcg cctcgctggc gtacctcgcg gtgttcgtcg tggccgggct cgatcaccgc
      781 gccggctggt cgcgcgtgcc ggtggctacg gtggtcgccg gcgacgcgct ggtcgtgctc
      841 gggatgttcg tggtgttccg ggtgttccgg gccaacacgt tcacgtcggc ggtgatcgac
      901 gtcgaccgcg atcagcagct ggtgtcgtcc gggccctacg cggtggtccg ccacccgatg
      961 tacgccggcg cgctcgtgat gatggccggt gtgccgatcg cgctgggctc gtggtgggcc
     1021 ctcgtcccgg tggtcgcgat gaccgcggtg atcgtggcgc gcctgctcga cgaggaagcg
     1081 ctgctcgcgg cccgcctgcc cggctacgcc gagtaccgca gccgggtgca gcagcggctg
     1141 gtgccgcgcg tgtggtgagg agcagtacac tcgaggcatg gacgcgctgc gcgacgtggc
     1201 tccccgcccc gaggcctcga cggagtcgac ccgcgactcg atgcgcgact cgatccgcga
     1261 tctcggcgcg cggttcgagg cccgcgcggt caacgtcgcc gagcggatcg acgtgcgcgg
     1321 catcgatccg cggctgtcga accacctgcc ggtcacggtc gcggtcggct ccggctgcgc
     1381 gatgctgttt cgctccgggg cggtcgtgtt ctttgcggtc gacaccgtgg cgcaggagcg
     1441 gttccagcac gacctcgggc cgcggatcca cggcccctac caggtgccgg agagcgagcg
     1501 cgcgacgatc cggatcggcg acgccgacgc ggtcgagccc gacgcgctgg tgatcaagga
     1561 gtcgtcgatc gagcgcatgc aggtgatcgc ggagatcctc gccaagagcg tcgtgctggc
     1621 gcgcaacgag caggagatcg gcacggcgtt cgacgcgatc gagccgctgg ccgccgagat
     1681 gaagcgcgcg ccgcgcaagc tgccgtggcg gcacagcgac ctggtgcgcc acatcggcga
     1741 ggcgatgctg gtcgagcacc agctggtcga tcgcgccgag gtgctggaga agccggagct
     1801 cttgtgggac cgcgccgacc tcgatcggct gtacgcgcgc ctcgaggacg agtacgagat
     1861 ccgcgagcgc cacctggtgc tggagagcaa gctgtcgctg gtcaccaccg cggcgcgcac
     1921 gatgctggag ctcaaccagg cgcggcgctc gctcaacgtc gagtactaca tcctcgcgct
     1981 gatcctcgtc gaggtcgtgc tcgcgctgta ccagatcttc cggctgtgag ccgccggtca
     2041 ccgcgggcgg agctgggtct ggaccatcgc ggcgagcgtg tggcggacct cgtcgtcgag
     2101 ctcgcgcgac ggctcgcggc cgagcgcggg ggcgagcgtg tgcttggcga gcgcgtagcc
     2161 gtacgcggcc gacaccgcgg tgagcagcgt gatctcgacg tcgcgcagca gcgcggggtc
     2221 cggctcgggt ctgagcgcgc gcgcgacctg ctcggcgatc accgcgaggc cgcgctcctg
     2281 gagcgcgaac gcgtggctcg cc
//
LOCUS       DASVOR010002215         1984 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_53687,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002215 DASVOR010000000
VERSION     DASVOR010002215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1984)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1984)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1984
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53687"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VF516_41090"
     CDS             <1..199
                     /locus_tag="VF516_41090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114217.1"
                     /translation="TPELPGFGEYLVAAGILTRERLRAAQVYQRSMRVQLSTAIVTLG
                     LASPQRIEWAAVAHHSQLPRG"
     gene            290..>1984
                     /locus_tag="VF516_41095"
     CDS             290..>1984
                     /locus_tag="VF516_41095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HEX8114218.1"
                     /translation="MPAAGDDAERERIAEDMRRVHNWRRWGPYLAERQWGTVREDYSA
                     TGEVWTYFPHDHARSRVYRWGEDGLLGICDRECRVCFALALWNGRDPILKERLFGVTG
                     AEGNHGEDCKELYYYLDATPTSSYLKALYKYPHDAYPYAALVDGNRARGRGDRELEIE
                     DTGVFERGYWDVQAEYAKAGPDDVLIRITVTNRGDAARLHVLPTVWLRNTWRWGRTGE
                     GYDPAGRIERAGAAGVRGVAGVQGAASVQIIQPTLGRFRLDCDGEPALLFTDNETHSE
                     RLWGVPACSRYTKDAFHRRVVGGELAAVNPALEGTKCAAWYVLDVPAGASRSLRLRRA
                     AEAGAGTGAAARPFDEFDAIFARRIAEADHYHRAVRDTAMTDEERRVVRQADAGLVWS
                     RQFYHYIVEHWLEGDPGQPPPAQQRLRGRNRTWQNLWARDVISMPDAWEYPWFAAWDL
                     AFHCVAMARFDPQFAKHQLLLLCREWYMHPNGQLPAYEFAFGDVNPPVHAWAVWRVYQ
                     LAGEAGYGYDREFLERAFDKCLINFTWWVNREDAEGDNLFTGGFLGLDNVGVFDRSQ"
BASE COUNT          274 a          713 c          726 g          271 t
ORIGIN      
        1 gacgccggag ctgccggggt tcggcgagta cctcgtggcc gccggcatcc tgacccgcga
       61 gcggttgcgc gccgcgcagg tctaccagcg ctcgatgagg gtgcagctgt cgaccgcgat
      121 cgtcacgctc ggtctggcca gcccgcagcg tatcgagtgg gccgccgtcg cccaccattc
      181 gcagctgccg cgcggctagc tcacctggtc tcgccgccga gccggccttg cggcggtatc
      241 acggtggacg cgccgccgcc ggccgcgcgc taggctacgt tcgggccaca tgccagcagc
      301 gggtgacgac gcggagcgcg agcggatcgc cgaggacatg cggcgcgtcc acaactggcg
      361 gcgctggggg ccgtacctgg ccgagcgcca gtggggcacg gtgcgcgagg actactcggc
      421 gaccggcgag gtctggacct actttccgca cgaccacgcg cgcagccggg tctaccgctg
      481 gggcgaggac ggcctgctcg ggatctgcga ccgcgagtgc cgggtgtgct tcgcgctggc
      541 gctgtggaac ggccgcgatc cgatcctcaa ggagcggctg ttcggggtca ccggcgccga
      601 gggcaaccac ggcgaggact gcaaggagct gtactactac ctcgacgcga ccccgacgtc
      661 gtcgtacctc aaggcgctgt acaagtaccc gcacgacgcc tacccgtacg ccgcgctggt
      721 cgacggcaac cgcgcgcgcg gccgcggcga tcgcgagctc gagatcgagg acaccggcgt
      781 gttcgagcgc ggctactggg acgtccaggc cgagtacgcc aaggccgggc ccgacgacgt
      841 cctgatccgg atcaccgtca ccaaccgcgg cgacgccgcc cggctccacg tcctgccgac
      901 ggtctggctg cgcaacacct ggcgctgggg gcgcaccggc gagggctacg atccggccgg
      961 acggatcgag cgcgccggtg ccgccggcgt gcggggggtc gccggcgtgc agggggccgc
     1021 cagcgtgcag atcattcagc ccacgctcgg ccggttccgg ctcgactgcg acggcgagcc
     1081 ggcgctgctg ttcaccgaca acgagaccca cagcgagcgg ctgtggggcg tgcccgcgtg
     1141 ctcgcgctac accaaggacg cgtttcaccg ccgcgtggtc ggcggcgagc tcgccgcggt
     1201 caacccggcg ctcgagggca ccaagtgcgc cgcgtggtac gtgctcgacg tcccggccgg
     1261 cgcgtcgcgc agcctgcggc tccggcgggc cgccgaggcg ggggctggga ccggagccgc
     1321 ggcgcggccg ttcgacgaat tcgacgcgat cttcgcccgc cggatcgccg aggccgacca
     1381 ctaccaccgc gcggtgcgcg acacggcgat gaccgacgag gagcgccgcg tggtgcgcca
     1441 ggccgacgcc ggcctggtgt ggtcgcggca gttttaccac tacatcgtcg agcactggct
     1501 ggagggcgat ccggggcagc cgccgccggc ccagcagcgg ctgcgcggcc gcaaccgcac
     1561 ctggcagaac ctgtgggcgc gcgatgtcat cagcatgccc gacgcgtggg agtacccgtg
     1621 gttcgccgcg tgggacctcg cgttccactg cgtcgcgatg gcgcggttcg atccgcagtt
     1681 cgccaagcac cagctgctgc tcttgtgccg cgagtggtac atgcacccca acggccagct
     1741 gccggcctac gagttcgcgt tcggcgacgt caacccgccg gtccacgcct gggcggtgtg
     1801 gcgggtctac cagctcgccg gcgaggccgg ctacggctac gaccgcgagt tcctcgagcg
     1861 cgcgttcgac aagtgcctga tcaacttcac gtggtgggtc aaccgcgagg acgccgaggg
     1921 cgacaacctg ttcaccggcg ggttcctcgg cctcgacaac gtcggcgtgt tcgatcgctc
     1981 ccag
//
LOCUS       DASVOR010002216         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_432381,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002216 DASVOR010000000
VERSION     DASVOR010002216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432381"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /gene="istB"
                     /locus_tag="VF516_41100"
     CDS             <1..715
                     /gene="istB"
                     /locus_tag="VF516_41100"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEX8114219.1"
                     /translation="RWLRLPGMAQLISSLLEQAKNDNLTDLEILHRLCDEERASRMKS
                     AVDRRIKDAKFPELNTVDGFDFDFDPVRKKLRARYLALHDLTFLDKGINPLFIGHPGT
                     GKTFLARALAYRACQATRRVVFTTATRMLNDLAGAEIHGQLERALRRYCRADFLVIDD
                     FAVLAMDPAQANLAFQVISERYEYHRSTCITTNRLFKDWPKVFPDALNAQVIAERFTE
                     RSERFILDGKGFRPTKNAT"
     gene            957..>1827
                     /locus_tag="VF516_41105"
     CDS             957..>1827
                     /locus_tag="VF516_41105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114220.1"
                     /translation="MTLTAKHAAAQWFLCQLKLKQPQVNVHAWYDADGTYVVVADNAA
                     SARSLDGESLDKWIQTHKPIGLQLRLADSPPNNSEKLPEQSLEQFLLPAGPVIWLRDL
                     YAELAFLVPRDFPEFKLESGSGTLDVIVERPLSESETSTLASAMAAVQITRPYSVRVE
                     QPFASDSFRLKIPGDLRLLPSRDLPREAGRALRHLYEEDEDRWKDARDRLYSSESTDP
                     SHLLPSSWAPGPSRCVLPTSVFEAHNIRNYLSLYREVVLTTPLLTSHEQIFASLSCSE
                     QELCRLAAAGRVRF"
BASE COUNT          362 a          614 c          498 g          353 t
ORIGIN      
        1 gcgatggctg cgcctccccg ggatggcgca gctgatctcg tcgctcctcg agcaggccaa
       61 gaacgacaac ctgaccgacc tcgagatcct ccaccgactc tgcgacgagg agcgcgccag
      121 tcgcatgaag agcgccgtcg atcgccgcat caaggacgcc aagttccccg agctcaacac
      181 cgtcgacggc ttcgacttcg acttcgaccc cgttcgcaag aagcttcgcg ctcgctacct
      241 cgcgctccac gacctcacgt tcctcgacaa gggcatcaac ccgctgttca tcggccaccc
      301 cgggaccggc aagaccttcc tcgctcgggc cctcgcgtat cgcgcctgcc aggccacgcg
      361 gcgcgtcgtc ttcaccaccg cgacccgcat gctcaacgat ctcgccggtg ccgagatcca
      421 cggtcagctc gagcgcgcac tccgtcgcta ctgccgtgcc gacttcctgg tcatcgacga
      481 tttcgcggtg ctggcgatgg acccggcaca ggccaacctc gccttccagg tcatctccga
      541 gcgctacgag taccatcgct cgacctgcat cacgaccaac cggctgttca aggactggcc
      601 caaggtcttc cccgacgcgc tcaacgccca ggtcatcgcc gagcgcttca ccgagcgctc
      661 cgagcgcttc atcctcgacg gaaaggggtt tcgaccgacg aagaacgcaa cctgactctg
      721 ccccgatcga gcatcatgct cggcttcgct cggttcgcac cgcgaactcg agatcctgcg
      781 cgtgccgccg cggcatcgtc gaggcgaaga accgtccgcg cgccaccggg tgggtccctg
      841 gatcccggtg acgccggtgg atctggtacc cacgctcgcc ggtggcgaca cgtggggtgg
      901 acatatcgac gcaaacgcaa gatgatgcta ctcattgacc aacgcacgca ccgggggtga
      961 ccttgacagc gaagcatgcc gcagcacagt ggttcctctg ccagctgaag ttgaagcaac
     1021 cgcaagtaaa tgtgcacgcg tggtacgatg cggacggtac atatgtcgtc gttgctgata
     1081 acgcggcttc cgcgaggtcg cttgacggcg agagcctcga caagtggatc cagactcaca
     1141 aaccgatcgg cctgcagtta cgtctcgcgg actcacctcc taacaatagc gaaaaactcc
     1201 ctgagcaatc tctcgagcaa ttcttactac ctgcgggtcc cgtaatatgg ctgcgcgatc
     1261 tctacgcaga actggcgttc ctagttccac gagacttccc tgagttcaag cttgaatccg
     1321 gatccggaac gctagatgtc attgtcgagc gcccgttgtc ggaaagtgag acctctacac
     1381 tagcgtcagc gatggctgcg gtgcagataa cgcggcccta ctccgtacgt gtcgagcagc
     1441 cgttcgcgtc cgattccttt cgactcaaaa tccctggcga cctacggctg cttccaagtc
     1501 gtgatcttcc gcgcgaagca ggtcgggcgc tacgtcacct ttacgaagaa gacgaggatc
     1561 gatggaagga cgcccgcgat cgcttgtatt caagtgaaag tacagatccg agccacctgc
     1621 tcccttcaag ctgggcgcct ggcccatcac gttgcgtgct tccgacatcg gtctttgaag
     1681 cacacaatat tcgcaactac ctatcgttgt accgtgaagt tgtactaaca acccctttgc
     1741 ttacgagtca tgagcagatc tttgcatcgc tttcgtgcag cgagcaggaa ctctgccgtt
     1801 tggcagcggc aggtcgagtc cgattct
//
LOCUS       DASVOR010002217         4420 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_462085,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002217 DASVOR010000000
VERSION     DASVOR010002217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4420
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462085"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1920)
                     /locus_tag="VF516_41110"
     CDS             complement(<1..1920)
                     /locus_tag="VF516_41110"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEX8114221.1"
                     /translation="MIRLVGFLILARVALVAGSAAAQPAPPVTWNEFDIEGELQDSPD
                     TVRTLLAPVMTRRTSLTDEARADITATAGQFGYQVVSLTTRDTPTGTHAVLRLSPRPL
                     VRKVDVDIKQPLFTILLDDQVRRRMRTRTGSYLAWDPAERKRELDSEAQQLADYLHFD
                     EGYFDATVQHEERERRGGIELTIRIKLGDEYRTGAIRVASPEALAATTIDRASVTGQF
                     RHKTVLDFVFGAARFTRARHLEDIQHVVELFQRRGYPAVRVHTSFEQEPASAFNRRTH
                     AVDFTVTIDPRRRIDVRFEGNPESVTDEQLRGQLTFNAAASSDDVEAGNSARAIVSYL
                     QSRGWFDAHVTWVRDRTQNPPLDTITFQLELGRQRPVRSIEFVGNSVLHTDQLENVIG
                     TTQEKLSTSLFGGNTNATSAQLAADVDRISQFYRSAGYRDARVRVEAAANPAAFGSAA
                     LTAALAATDQGDGLYVRYVIDEGEPTLLTGVEVALAGGDPTIHDDDQRRLCKLVLAEL
                     AGLYGNDQLATPTNTARCAASVSPAHKLMFREDAAAATRDQLKDRLFSHGMPRAEVGY
                     AAQVSGPRQVTARYTLANTQILHIGTVVVRGNFKTRDGIILSELGLRRGARLTQDALA
                     EGARRLRNTGLFDAVS"
     gene            complement(1917..>4420)
                     /locus_tag="VF516_41115"
     CDS             complement(1917..>4420)
                     /locus_tag="VF516_41115"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEX8114222.1"
                     /translation="TLHLQGLPSAPQATGAVQLLRAWLANGFLGDAQLAVKPATVGGV
                     AGVEFTGSALAGRLTITGSLGTAAPYPLELAISGRRLELDPYLDLQALLQLPDPVQAW
                     ASGTVTLRTELAPARPIEPEIWIELDELSAQLDHRASDGRVTPLIVRIKDQAAQHRVA
                     LSVRATPTSFELACRDPRQPGGRIECPAVLETPAGDITIQGHATTSSVQVTADGPLDL
                     GKLRVLLDRRFDYVAGRVRLAASLTGQFNHPAFTAEIDLDPDQVWTRNEAERQARREA
                     AATHRPYREAPILPDEKPVVLRPSGSDTVLTAPRGLIKLANGSIGFTDVLVQIKDDRH
                     VDEQGDLHIAGNIALDGVTPVGWSVFLSGRLAGKMLQIALPNLVAQADGLIDIDDQLQ
                     LTGKGPLPAVQGALLFDKADPLSLIPRGVRRELTFRDGKIEIETSDEQRTYTIGIAGV
                     EGTIDDGKLTNIRGTLKIQNGDLASASLRLSADSIPFHIPQTLDLLLSASDIRVTLDD
                     KAAPWRVKGTLRVIDGAFFRNFEITDKIQAIGVNVAPTRPFWEEYPTLGSAELDLKLD
                     VGLFAVKSNIATIEFQGDNLHIGGSPRDPRLYGQIRVTHGDFHIPGARAAFNRTSGSV
                     DFAENQPASNPELQITSEADYRDLSGQDHVITARISGTLQQLSWDLKTSTGYNKSQTL
                     SLLVLGRSPDQLRRSLGDQNIGTDPTRIDPTTNPSQGIADQIVKDLAGDWVSDLLGSS
                     LTKLTGLDVLRIEIGFGSIGFHAEKKMLENLKAFGDAEQTIRGTTIKLRAELRTPFVV
                     SLQGGYLHQHYYDPAEQDIVDYDVKLVYRYRLFIP"
BASE COUNT          566 a         1459 c         1694 g          701 t
ORIGIN      
        1 gctgaccgca tcgaacagcc cggtgttgcg cagccgccgc gcgccctcgg cgagcgcgtc
       61 ctgggtcagc cgcgcgccgc gccgcaggcc gagctcgctg aggatgatcc cgtcgcgggt
      121 cttgaagttg ccgcggacca cgaccgtgcc gatgtgcagg atctgggtgt tggccagggt
      181 atagcgcgcg gtgacctggc gcgggccgct gacctgggcg gcgtagccga cctcggcgcg
      241 cggcatgccg tggctgaaca gccggtcctt gagctgatcg cgggtcgcgg cggcggcgtc
      301 ctcgcggaac atcagcttgt gggccggtga gaccgatgct gcgcagcgcg cggtgttggt
      361 cggggtcgcg agctggtcgt tgccgtacag cccggcgagc tcggcgagca ccagcttgca
      421 cagccggcgc tggtcgtcgt cgtggatcgt ggggtcgccg ccggcgagcg cgacctcgac
      481 gccggtcagc agggtcggct cgccctcgtc gatcacgtag cgcacgtaca gcccgtcgcc
      541 ctgatcggtc gcggcgagcg ccgcggtcag cgccgcgctg ccgaacgccg ccgggttggc
      601 ggcggcctcg acccggaccc gcgcgtcgcg gtagcccgcg ctgcggtaga actgcgagat
      661 ccggtcgacg tcggcggcga gctgcgccga ggtcgcgttg gtgttgccgc cgaacaggct
      721 ggtggacagc ttctcctggg tcgtgccgat cacgttctcg agctggtcgg tgtggagcac
      781 ggagttgccg acgaactcga tcgagcgcac cgggcgctgc cggccgagct cgagctggaa
      841 cgtgatcgta tcgagcggcg ggttctgggt ccgatcgcgg acccaggtga cgtgggcgtc
      901 gaaccagccc cggctctgga ggtacgacac gatcgcgcgc gccgagttgc cggcctcgac
      961 gtcgtccgag ctcgccgccg cgttgaacgt cagctggccg cgcagctgct cgtcggtcac
     1021 cgactcgggg ttgccctcga accggacgtc gatccggcgc cgcggatcga tggtgacggt
     1081 gaagtcgacc gcgtgggtcc ggcggttgaa cgccgacgcg ggttcctgct cgaagctggt
     1141 gtggacgcgg accgccgggt agccgcggcg ctggaacagc tcgaccacgt gctggatgtc
     1201 ctcgaggtgg cgggcgcggg tgaaccgcgc ggcgccgaac acgaagtcga ggacggtctt
     1261 gtgccggaac tggccggtca cgctggcgcg gtcgatcgtc gtcgcggcca gcgcctcggg
     1321 gctggcgacc cggatcgccc cggtccggta ctcgtcgccg agcttgatcc ggatcgtgag
     1381 ctcgatcccg ccgcggcgct cgcgctcctc gtgctggacg gtggcgtcga agtagccctc
     1441 gtcgaagtgc aggtagtcgg cgagctgctg ggcctcgctg tcgagctcgc gcttgcgctc
     1501 ggccgggtcc cacgccaggt agctcccggt ccgggtccgc atccggcggc ggacctggtc
     1561 gtcgagcagg atcgtgaaca gcggctgctt gatgtcgacg tcgaccttgc ggaccagcgg
     1621 gcgcggcgac agccggagca ccgcgtgggt gccggtcggg gtgtcgcgcg tggtcaggct
     1681 gaccacctgg tagccgaact gcccggcggt cgcggtgatg tcggcgcgcg cctcgtcggt
     1741 cagcgaggtc cggcgggtca tcaccggcgc cagcagcgtg cgcaccgtgt ccggcgagtc
     1801 ctgcagctcg ccctcgatgt cgaactcgtt ccaggtcacc ggcggcgccg gctgcgccgc
     1861 ggcggacccc gcgaccagcg cgacccgagc gaggatgagg aagccgacca gccggatcac
     1921 gggatgaaca ggcgatagcg atagaccagc ttgacgtcgt agtccacgat gtcttgctcg
     1981 gcgggatcgt agtagtgctg gtggaggtag ccgccctgca ggctgaccac gaacggggtg
     2041 cgcagctcgg cgcgcagctt gatcgtcgtg ccgcggatcg tctgctccgc gtccccgaac
     2101 gccttgaggt tctccagcat cttcttctcg gcgtggaacc cgatcgagcc gaacccgatc
     2161 tcgatgcgca gcacgtcgag cccggtcagc ttggtcagcg agctgccgag cagatcggac
     2221 acccagtcgc cggcgaggtc cttgacgatc tggtcggcga tgccctggct cgggttggtc
     2281 gtcggatcga tccgggtcgg atcggtgccg atgttctggt cgccgagcga gcgccgcagc
     2341 tggtccgggc tgcggccgag cacgagcagc gacagggtct gcgacttgtt gtagccggtc
     2401 gaggtcttga ggtcccagct cagctgctgc agcgtgccgc tgatcctcgc ggtgatcacg
     2461 tggtcctggc cggacaggtc gcggtagtcg gcctcgctgg tgatctggag ctcggggttg
     2521 ctggcgggct ggttctcggc gaagtcgacc gagccgctgg tccggttgaa cgcggcccgg
     2581 gcgcccggga tgtggaagtc gccgtgggtg acccggatct gaccgtacag ccgggggtcg
     2641 cgcggcgagc cgccgatgtg gaggttgtcg ccctggaact cgatggtcgc gatgttgctc
     2701 ttgaccgcga acagcccgac gtcgagcttg aggtcgagct cggcgctgcc cagcgtcggg
     2761 tactcctccc agaacggccg ggtcggcgcg acgttgacgc cgatcgcctg gatcttgtcg
     2821 gtgatctcga agttccggaa gaacgcgccg tcgatcacgc gcagcgtgcc cttgacccgc
     2881 cacggcgcgg ccttgtcgtc gagcgtgacc cggatgtcgc tggccgacag caacagatcg
     2941 agggtctgcg ggatgtggaa cgggatgctg tcggccgaca gccggagcga cgcgctcgcc
     3001 agatcgccgt tctggatctt cagcgtgccc cggatgttgg tcagcttgcc gtcgtcgatc
     3061 gtgccctcga cgcccgcgat cccgatcgtg taggtccgct gctcgtccga ggtctcgatc
     3121 tcgatcttgc cgtcgcggaa cgtcagctcg cggcgcacgc cgcgcgggat cagcgacagc
     3181 gggtcggcct tgtcgaacag cagcgcgccc tggaccgcgg gcagcggccc cttgccggtc
     3241 agctggagct ggtcgtcgat gtcgatcagg ccgtcggcct gggcgaccag gttgggcagc
     3301 gcgatctgca gcatcttgcc ggcgagccgg cccgacagga acaccgacca gccgaccggc
     3361 gtgacaccgt cgagcgcgat gttgccggcg atgtggagat cgccctgctc gtcgacgtgg
     3421 cggtcgtcct tgatctggac cagcacgtcg gtgaagccga tcgagccgtt ggcgagcttg
     3481 atcaggccgc gcggcgcggt gagcacggtg tcgctgccgc tcggccgcag cacgaccggc
     3541 ttctcgtcgg gcaggatcgg cgcctcgcgg tacgggcggt gggtcgcggc cgcctcgcgc
     3601 cgggcctggc gctcggcctc gttgcgggtc cacacctggt cgggatcgag gtcgatctcg
     3661 gcggtgaacg cggggtggtt gaactggccg gtgagcgacg cggcgagccg gacccggccg
     3721 gcgacgtagt cgaaccgccg gtcgagcagg acgcgcagct tgccgagatc gagcgggccg
     3781 tccgccgtga cctggaccga gctggtcgtg gcgtggccct ggatcgtgat gtcgcccgcc
     3841 ggggtctcga gcaccgcggg gcactcgatg cgcccgccgg gctgccgggg gtcgcggcac
     3901 gcgagctcga acgacgtcgg cgtcgcgcgc accgacagcg cgacgcggtg ctgcgcggcc
     3961 tggtccttga tccgcacgat cagcggggtg acccggccgt cgctcgcgcg gtggtcgagc
     4021 tgcgccgaca gctcgtcgag ctcgatccag atctcgggct cgatcggccg ggcgggcgcg
     4081 agctcggtgc gcagcgtgac ggtgccggag gcccaggcct ggaccggatc gggcagctgg
     4141 agcagcgcct ggagatcgag gtaggggtcg agctcgagcc gccggccgct gatcgcgagc
     4201 tcgagcgggt agggcgcggc ggtgccgagc gagccggtga tcgtcagccg gccggcgagc
     4261 gccgagccgg tgaactcgac gcccgcgacc ccgccgaccg tggcgggctt gaccgcgagc
     4321 tgggcatcgc cgaggaagcc gttggccagc cacgcgcgca gcagctggac cgcgccggtc
     4381 gcctggggcg cgctcggcag gccctggaga tggagggtca
//
LOCUS       DASVOR010002218         2062 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_402979,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002218 DASVOR010000000
VERSION     DASVOR010002218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402979"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2062)
                     /gene="rnr"
                     /locus_tag="VF516_41120"
     CDS             complement(<1..>2062)
                     /gene="rnr"
                     /locus_tag="VF516_41120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEX8114223.1"
                     /translation="IAAPVTRPGKPPPPREEYDPETGEVREIGDATPPGRDDRIIGRI
                     TVHPAGYGFVATEAGETVFVPAKFRATSLDGDRVAVDTWPGVRGTEGRVSEVLARGRA
                     RLTGILRRVGRAVYLEPDDPRISSDYGRVALDDAPLGRDGDAVVIEITRYPDAARREL
                     AGKLLKVLGDPDDPRTEIEKILACAVIPIEFPDEAAAQARATPQELGALDLADRIDLR
                     DRRFATIDPETARDFDDALCIEDGPHGGPRVWVAVADVSHYVRWDDALDKEAQIRGVS
                     VYLPDRVIPMLPHQLSAGICSLNPLVDRCAMVVRLDYSHDADLVDVGYAAAVIKSKAR
                     LDYPGVAAALEGDFRGRRQEYREWAGELSRLDALARQLRKKRQARGALELELSEPKVV
                     LDADDPRLVRDVVKAKGDPAVKQAYQLVEEFMIAANEAVGHFFRRRGAPTVWRVHAPP
                     KADRVANLAELLGAYGIAVDVEQATTPLGMKHVLDQIADKPGAQALSFLVLRTLTQAV
                     WDTVPIGHFGLASGDYLHFTSPIRRYPDLLVHRLLKYHLHREGHASGGGYTKPPPPVT
                     DLSTLAHASSEHERRAMEAEREAVAMYRAYLMRDQVGQRFDGVVSAVTSFGAFVEIEE
                     PYVEGLIKLDSLGDDTFSFDEVHMRLSGQRTGTTISLGDKVTVEINNVSVVRRRIDFT
                     LIAAQ"
BASE COUNT          268 a          704 c          798 g          292 t
ORIGIN      
        1 tctgcgcggc gatcagggtg aagtcgatcc ggcggcggac caccgagacg ttgttgatct
       61 cgacggtgac cttgtcgccc aggctgatcg tcgtgccggt ccgctggccg gacagccgca
      121 tgtggacctc gtcgaagctg aacgtatcgt cgccgagcga gtcgagcttg atcaggccct
      181 cgacgtaggg ctcctcgatc tcgacgaacg cgccgaagct ggtgaccgcg gacaccacgc
      241 cgtcgaaccg ctggccgacc tggtcgcgca tcaggtaggc gcggtacatc gcgaccgcct
      301 cgcgctcggc ctccatcgcg cggcgctcgt gctcgctcga cgcgtgggcc agcgtggaca
      361 gatcggtgac cggcggcggc ggcttggtgt agccgccgcc cgaggcgtgg ccctcgcggt
      421 ggaggtggta cttgagcagg cggtggacga gcagatcggg gtagcggcgg atcggcgagg
      481 tgaagtgcag gtagtcgccc gacgccaggc cgaaatggcc gatcgggacg gtgtcccaca
      541 ccgcctgggt cagcgtgcgc aggaccagga acgacagcgc ctgggcgccc ggcttgtcgg
      601 cgatctggtc gagcacgtgc ttcatgccga gcggcgtcgt cgcctgctcg acgtcgaccg
      661 cgatgccgta ggcgccgagc agctcggcga ggttggcgac ccggtcggcc ttgggcggag
      721 cgtggacccg ccacaccgtc ggggcgccgc gccggcggaa gaagtgaccg accgcctcgt
      781 tggcggcgat catgaactcc tcgaccagct ggtaggcctg cttgaccgcg gggtcgccct
      841 tggccttgac cacgtcgcgg accagccggg ggtcgtcggc gtcgagcacg accttgggct
      901 cggacagctc gagctcgagc gcgccgcgcg cctggcgctt cttgcgcagc tggcgggcga
      961 gcgcgtcgag ccgcgacagc tcgccggccc actcgcggta ctcctggcgc cggccgcgga
     1021 agtcgccctc gagcgcggcg gcgacgccgg ggtagtcgag ccgggccttg ctcttgatca
     1081 ccgcggcggc gtagccgacg tcgaccaggt cggcgtcgtg gctgtagtcg agccggacga
     1141 ccatggcgca gcggtcgacc agcgggttga gcgagcagat gccggccgac agctggtgcg
     1201 gcagcatcgg gatcacgcgg tccggcaggt agaccgacac gccgcggatc tgggcctcct
     1261 tgtcgagcgc gtcgtcccac cgcacgtagt gcgagacgtc ggcgaccgcg acccacacgc
     1321 gcgggccgcc gtgcgggccg tcctcgatgc acagcgcgtc gtcgaagtcg cgcgcggtct
     1381 cggggtcgat cgtggcgaac cggcgatcgc gaagatcgat gcggtcggcg aggtcgagcg
     1441 cgccgagctc ctggggggtc gcgcgggcct gcgcggcggc ctcgtcgggg aactcgatcg
     1501 ggatcaccgc gcacgccagg atcttctcga tctcggtgcg cgggtcgtcg ggatcgccga
     1561 gcaccttgag cagcttgccg gcgagctcgc ggcgcgcggc gtcggggtag cgcgtgatct
     1621 cgatgaccac ggcgtcgcca tcgcggccga gcggcgcatc gtcgagcgcg acccggccgt
     1681 agtccgacga gatccgcggg tcgtcgggct cgaggtagac ggcgcggccg acccggcgca
     1741 gtatgccggt gagccgggcg cggccgcggg cgaggacctc gctgacccgg ccctcggtgc
     1801 cgcgcacgcc gggccaggtg tcgaccgcga cgcgatcgcc gtcgagcgag gtggcgcgga
     1861 acttggcggg cacgaacacg gtctcgccgg cctcggtggc gacgaagccg tagccggccg
     1921 ggtgcacggt gatccgcccg atgatgcgat cgtcgcggcc cggcggcgtg gcgtcgccga
     1981 tctcgcggac ctcgccggtc tccggatcgt actcctcgcg cggcggcggc ggcttgcccg
     2041 gccgggtcac cggcgccgcg at
//
LOCUS       DASVOR010002219         3169 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_112629,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002219 DASVOR010000000
VERSION     DASVOR010002219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112629"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="VF516_41125"
     CDS             <1..207
                     /locus_tag="VF516_41125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114224.1"
                     /translation="LSPAVVKVLYQALARIRADGVTLVFVEQDVHRALAFSDHLVCLH
                     KGTVRLSGASRGRSLDEVRRAYFG"
     gene            210..1097
                     /locus_tag="VF516_41130"
     CDS             210..1097
                     /locus_tag="VF516_41130"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8114225.1"
                     /translation="MQLVETLLNGCLLGGLYGLIGLGLSINFGTMRVVHLAHGDLIVL
                     AAYFLVVVLVPAGIPPLAAAAIVVPVMFGVGWVLQQLIVQYSLGRQLDRALGRGVLPP
                     LIVTFGVSIVLQNVLQHCATADARTLQSSLASSGVTLGGIAIPTVRLVDFAVGLGCLG
                     LLLLGLGRTALGRALRATSDDLDTARLLGIDPRRMYALAAGISAATGGVAGVLFAATS
                     TFYPSTGSEVLLVAFEVVVVGGLGSLVGTVLGGLLLASAQLLGAHFLGPSYALLTGHV
                     VVLGVLVFRPQGLLPRTAP"
     gene            1094..2095
                     /locus_tag="VF516_41135"
     CDS             1094..2095
                     /locus_tag="VF516_41135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8114226.1"
                     /translation="MTARTTARAHGWLRAAIAAVLVIVLVACPAWGEGGARATLIVFA
                     YYAALAQMWNLLAGYAGLVTFGQQLFIGLGGYTLAVGAERLGLGVWPSVALAAVVAGA
                     LAVPVGALTFRLKGGYFAVGSWLVSEVFRLSVGNSAYLGHASGLFIRPARSVDPWTVY
                     YLAIGLLIAMVALVFVTLRSRAGLALMAIRDAEDAAAGLGVHVWRLKLLVFVVAAAGT
                     GLCAALITVQTPFIQPNAAFSIQWSAAVSFMAVVGGLGTVEGPLVGAAVYVVLQDQLR
                     ATGEVHLIVLGAVSVIVMLVAPRGLAGFVRDRFGWTLFPVARRPPHGAGGSTGPRTA"
     gene            complement(2303..3028)
                     /locus_tag="VF516_41140"
     CDS             complement(2303..3028)
                     /locus_tag="VF516_41140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114227.1"
                     /translation="MRRAIALGLTLALAAPGCHRDGTAAPQPNPEQPTRNQAPPDKAA
                     EGSMTNTELEQKLVHDELGMTFVWPQHDNTIAAIWSLPGNPRLLESVLNDPHAAGRAR
                     FIAAEVLFAKDFSFIDRTSRDGLARLYAEALVKRYTGQANAWGLLWDGNDAGPAGGRF
                     ILLGDDAIPALVHLLDDTTVVDWYAGSEEATVGNGYKYRIKDFAAFYLGRITGKPIAF
                     HDEPAARDREIAQLRDTLRNRGK"
BASE COUNT          333 a         1161 c         1124 g          551 t
ORIGIN      
        1 ctgtcgccgg cggtggtcaa ggtgctctac caggcgctcg cccggatccg cgccgacggc
       61 gtgaccctgg tgttcgtcga gcaggacgtc caccgcgcgc tcgcgttctc cgatcacctg
      121 gtctgcctcc acaagggcac cgtgcggctg tccggcgcgt cgcgcgggcg ctcgctcgac
      181 gaggtgcgcc gcgcctactt cggttgacca tgcagctggt cgagaccctc ctcaacggat
      241 gtctgctcgg tggcctgtac ggcctgatcg ggctcggcct gtcgatcaac ttcggcacga
      301 tgcgcgtcgt ccacctcgcg cacggcgacc tgatcgtgct cgccgcgtac ttcctcgtcg
      361 tcgtgctggt gccggccggg atcccgccgc tggccgccgc ggcgatcgtc gtgccggtga
      421 tgttcggcgt cggctgggtg ctgcagcagc tgatcgtcca gtacagcctc gggcgccagc
      481 tcgaccgggc gctcggccgc ggcgtgctgc ccccgctgat cgtcacgttc ggcgtctcga
      541 tcgtgctgca gaacgtgctc cagcactgcg ccaccgccga cgcgcgcacg ctgcagagct
      601 cgctcgcgtc gtcgggcgtg acgctgggcg gcatcgcgat cccgacggtg cgcctggtcg
      661 acttcgccgt cggcctcggc tgcctgggcc tcctgctgct cggcctgggc cgcaccgcgc
      721 tcggccgcgc gctgcgcgcg acctcggatg acctcgacac cgcgcgcctg ctcggcatcg
      781 acccgcggcg gatgtacgcc ctggcggccg ggatctcggc ggcgaccggc ggcgtggccg
      841 gcgtgctgtt cgccgcgacc tcgacgttct atccgtcgac cggctccgag gtgctgctgg
      901 tcgcgttcga ggtcgtcgtg gtcggtgggc tcggcagcct ggtcggcacc gtcctcggcg
      961 gcctcctgct cgcgagcgcg cagctgctcg gcgcgcactt cctgggtccg agctacgccc
     1021 tgctcaccgg gcacgtggtc gtgctcggcg tgctggtgtt ccgcccgcag ggcctcctgc
     1081 cgaggaccgc gccatgaccg ccagaacgac cgcccgggcg cacggctggc tgcgcgccgc
     1141 gatcgcggcc gtgctcgtga tcgtcctcgt cgcgtgcccg gcgtggggcg agggcggcgc
     1201 gcgcgcgacc ctgatcgtgt tcgcgtacta cgccgcgctg gcccagatgt ggaacctgct
     1261 cgccggctac gccgggctgg tcacgttcgg ccagcagctg ttcatcggcc tgggcggcta
     1321 caccctggcg gtcggcgccg agcggcttgg cctcggcgtg tggccatcgg tcgcgctcgc
     1381 cgcggtcgtc gccggcgcgc tcgccgtccc ggtcggcgcg ctgaccttcc ggctcaaggg
     1441 cggctacttc gccgtcggca gctggctggt ctccgaggtc ttccgcctgt cggtcggcaa
     1501 ctcggcgtac ctcggccacg cctcgggcct gttcatccgg ccggcgcgca gcgtcgatcc
     1561 gtggaccgtc tactacctcg cgatcggcct gctgatcgcc atggtcgccc tggtcttcgt
     1621 cacgctgcgc tcgcgcgccg gcctcgcgct gatggcgatc cgcgatgccg aggacgccgc
     1681 cgccggcctc ggcgtccacg tctggcggct caagctgctg gtgttcgtcg tcgccgccgc
     1741 gggcaccggc ctctgcgccg cgttgatcac cgtgcagacg ccgttcatcc agcccaacgc
     1801 cgcgttctcg atccagtggt cggcggcggt cagcttcatg gccgtggtcg gcggcctggg
     1861 caccgtcgag ggcccgctgg tcggcgccgc ggtctacgtg gtgctgcagg accagctccg
     1921 cgcgaccggc gaggtccacc tcatcgtgct cggcgccgtc tcggtgatcg tcatgctggt
     1981 cgcgccacgc ggcctcgccg gcttcgtccg cgaccgcttc ggctggacgc tgttccccgt
     2041 cgcccgccgg ccgccgcacg gcgcgggcgg aagcacggga ccccgcacgg cttgatgcgg
     2101 ccgccgcgcg atcgaggcag cgtcactgca tgtggaggac gccgttcgct ttctgcacgt
     2161 ggatgacgcc gttatccgtc gcgagcccac tgcccgggat ccctccgcca cgcacccacg
     2221 cgagctgctg cgcgcccagg cggtgaggcc gatcgcgcta ctggcgcaag ccgagcgacg
     2281 agtgtgggct gtggagcatc ggtcacttgc cgcggttgcg cagcgtatcg cgcagctgcg
     2341 cgatctcgcg gtcccgggcg gcgggctcat cgtggaacgc gatcggcttg ccggtgatcc
     2401 ggccgagata gaacgccgcg aagtccttga tccggtactt gtaaccgttg ccgacggtgg
     2461 cctcctcgct gccggcatac cagtcgacga cggtcgtgtc gtcgagcagg tggacgagcg
     2521 ccgggattgc atcgtcgccg aggaggatga accggccgcc ggcgggcccg gcgtcgttgc
     2581 cgtcccacaa caggccccag gcattcgcct ggccggtgta gcgcttgacc agcgcctcgg
     2641 cgtagagccg ggcgagcccg tcgcggctcg tgcgatcgat gaacgagaag tccttggcga
     2701 acagcacctc ggctgcgatg aaccgcgcgc ggccggcggc gtgcggatcg ttcagcacgc
     2761 tctccagcag ccgtgggttg ccgggcagag accagatcgc cgcgatcgtg ttgtcgtgct
     2821 gcggccacac gaaggtcatc ccgagctcgt catggacgag cttttgctcc agctctgtgt
     2881 tggtcatcga gccctccgcc gccttgtcgg gcggcgcctg gttgcgcgtg ggctgctccg
     2941 ggttcggttg gggggccgcc gtgccgtcgc gatggcatcc cggcgcggcg agtgcgagcg
     3001 tgaggcccag cgcgatcgct cggcgcatcg caggtatcac ggtcgttcgt ccttgagcgc
     3061 cgatctcggt acgtacccgc tgtgaccggt caagggcccc tgactgacct cgacccagac
     3121 cacctccacc tcgggtccag ggcccgggtc cgccgggacc ttccgggta
//
LOCUS       DASVOR010002220         4091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_112647,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002220 DASVOR010000000
VERSION     DASVOR010002220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112647"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..2186
                     /gene="glgB"
                     /locus_tag="VF516_41145"
     CDS             318..2186
                     /gene="glgB"
                     /locus_tag="VF516_41145"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF008967.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEX8114228.1"
                     /translation="MSLLTSDDLFLFNQGTHYRLYEKLGAHVVSGGTYFAVWAPNATA
                     VSVIGDWNAWRSGQDRLIPRASSGIWEVTVAGIGHGARYKFSIIGPDGVVRDKADPFA
                     ARSEHPPATASVVWQLRHDWGDAAWMAGRGARFARTAPISIYELHAGSWRRDHGEVLG
                     YVELGARLGDHVGRLGFTHVELMPVMEHPFYGSWGYQVTGYFAATTRYGAPEDLMAMI
                     DGLHQRGIGVIVDWVPAHFPTDAHGLGQFDGTHLYEHADPRRGFHPDWTSYIFNYGRH
                     EVRSFLISSAMSWLDRYHVDGLRVDGVASMLYRDYSRKPGEWVPNEDGSNHDRDAISF
                     LQQANRAIYTAFPDVQTIAEESTAWGGVSRPPEAGGLGFGYKWDLGWMHDTLGFVARE
                     PIHRRWHYNELTFRSLYAFTENFVLPLSHDEVVHGKRSLLAKLPGDPWQKYATLRLLY
                     GYQWTLPGKKLLFMGGDLGVWNEWNHDGELDWPIADHPAHAGIQRWIADLNATYRRYP
                     ALHVADCEPWGMRWLVADDRDACVLVWTRHGEAGDAPIVVVANFTPVPREGYRIGVPR
                     PGHWRELLNSDATVYGGSGLGNRGGLDTEPLACRGLEHSLVVTAPPLAVVVFAAQS"
     gene            2183..>4091
                     /locus_tag="VF516_41150"
     CDS             2183..>4091
                     /locus_tag="VF516_41150"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEX8114229.1"
                     /translation="MTDTPDKWICIHGHFYQPPRENPWLEAVEPQPSAHPYRDWNERI
                     TAECYRPNAAARVVDNGNQIIAIVDNYQRMSFDVGPTLLSWLEDSAPDVYEAVIAADR
                     ASCARFGGHGSALAQAYNHMIMPLASARDRATQVRWGIADFQRRFGRAPEGMWLPECA
                     VDTPSLEALAAEGIAFAVLAPHQAKAWRPPGGAWRTQPIDTGRAYRCALPSGRTIDLF
                     FYDGATSRAVAFERLLADGHQIIARMTARGAVEGGGPTLCHIATDGETYGHHHRYGDM
                     ALAWALQQVERGWDGTRLTNYAEFRARVPATWEVAIAEHTSWSCVHGTARWRDDCGCN
                     TGRAGWNQRWRRPLRDTFDWLRDQAAAALDNVGRILFRDPWAARDAYIAVVLERSAAA
                     RDQFLAAHTAHRLDPDERVRALSLMEMARHAMLMYTSCGWFFDDLSGIETVQCLQYAA
                     RVAELLEDVGGAPVEPELVDRLSAASSNLAEEGDGRQVFTRHVQPARVDPAKVCAHVA
                     VQALVEPEGQHSVDVYGHHVDFLDRIERRSGHTRMVAGTVRVRSRLTEIATSLCFAGL
                     HLGEQHVTGGVRPPPPQGAWAAIVEELTGAFRTADVFAAQRAIARHFPGHELSLSSLL
                     PGSRERVLGAVL"
BASE COUNT          566 a         1505 c         1449 g          571 t
ORIGIN      
        1 ccgcggtcag acgctcgctc gccggtgccg cctcctgcgc cttgcgccgg gccgccggag
       61 ccggcgctgc gaccggcggc gcgacgggcg cggtgggcgg cggtgactcg tggcatgcga
      121 caaggaggag gagggtagcg aaagctcgca tctctacacc atactctgag tcgtgcgtcc
      181 ctgggtgttc gaaccgtaac ggcaaataac ggtccattcc ggagcgatgc gccccgccgg
      241 aggagatttc gcagcgttat cgcgaactgg cgccaagggc tcgccccggc ggagatgctg
      301 ggtatcctgc cgccgccgtg tcgctcttga cctccgatga cctgttcctg ttcaaccagg
      361 gcacacatta ccgcctatac gaaaaactag gggctcatgt ggtgtccggt gggacgtact
      421 ttgccgtctg ggcgcccaac gcgaccgcgg tcagcgtgat cggcgactgg aacgcgtggc
      481 gcagcggtca ggatcggctg atcccgcgcg cgtcgtcggg gatctgggag gtgacggtcg
      541 cggggatcgg ccacggggcg cgctacaagt tctcgatcat cgggccggac ggcgtggtcc
      601 gcgacaaggc cgacccgttc gcggcgcgct cggagcaccc gccagcgacc gcgtcggtcg
      661 tgtggcagct ccgccacgac tggggcgacg cggcgtggat ggcgggccgg ggggcgcggt
      721 tcgcgcgcac cgcgccgatc agcatctacg agctgcacgc cgggtcgtgg cggcgcgatc
      781 acggcgaggt gctgggctac gtcgagctcg gcgcgcgact cggcgaccac gtcggccggc
      841 tcgggttcac ccacgtcgag ctgatgccgg tcatggagca cccgttctac ggctcgtggg
      901 gctaccaggt caccggctac ttcgcggcga ccacgcgcta cggcgcgccc gaggacctga
      961 tggcgatgat cgacggcctg caccagcgcg gcatcggggt gatcgtcgac tgggtgccgg
     1021 cgcacttccc gaccgacgcg cacggcctcg ggcagttcga cggcacgcac ctctacgagc
     1081 acgccgatcc gcggcgcggg ttccacccgg actggaccag ctacatcttc aactacggcc
     1141 gccacgaggt gcggtcgttc ctgatctcgt cggcgatgtc gtggctcgac cgctaccacg
     1201 tcgacgggct gcgggtcgac ggcgtcgcgt cgatgctgta ccgcgactac tcgcgcaagc
     1261 ccggcgagtg ggtgcccaac gaggacggct cgaaccacga ccgcgacgcg atcagcttcc
     1321 tgcagcaggc aaaccgcgcg atctacaccg cgttccccga cgtccagacc atcgccgagg
     1381 agtcgaccgc gtggggcggg gtgtcgcgcc cgcccgaggc cggcggcctg gggttcggct
     1441 acaagtggga cctcggctgg atgcacgaca cgctcggctt cgtcgcgcgc gagccgatcc
     1501 accggcgctg gcactacaac gagctcacct tccgctcgct gtacgcgttc accgagaact
     1561 tcgtcctgcc gctgtcgcac gacgaggtcg tccacggcaa gcgctcgctg ctcgccaagc
     1621 tgccgggcga tccgtggcag aagtacgcca cgctgcgcct gctctacggc taccagtgga
     1681 cgctgcccgg caagaagctg ttgttcatgg gcggcgacct cggggtgtgg aacgagtgga
     1741 accacgacgg cgagctcgac tggccgatcg ccgaccaccc ggcgcacgcc ggcatccagc
     1801 gctggatcgc cgacctcaac gcgacctacc ggcgctaccc ggcgctgcac gtcgccgact
     1861 gcgagccgtg ggggatgcgc tggctggtcg ccgacgaccg cgacgcctgc gtgctggtct
     1921 ggacgcgcca cggcgaggcc ggcgacgccc cgatcgtcgt ggtcgccaac ttcacgccgg
     1981 tgccgcgcga gggctaccgg atcggcgtgc cgcgcccggg ccactggcgc gagctgctca
     2041 actccgacgc gacggtctac ggcggcagcg ggctcggcaa ccgcggcggg ctcgacaccg
     2101 agccgctggc ctgccgcggc ctggaacact cgctcgtggt caccgcgccg ccgctcgccg
     2161 tcgtagtatt tgcggcgcaa tcgtgaccga cacgccggac aagtggatct gcatccacgg
     2221 ccacttctac cagccgccgc gcgagaaccc ctggctggag gccgtcgagc cccagccgag
     2281 cgcgcacccg taccgcgact ggaacgagcg gatcaccgcg gagtgctatc gccccaacgc
     2341 cgcggcccgc gtggtcgaca acggcaacca gatcatcgcg atcgtcgaca actaccagcg
     2401 gatgagcttc gacgtcgggc cgaccctcct gtcgtggctc gaggacagcg cgcccgacgt
     2461 ctacgaggcg gtgatcgccg ccgatcgcgc gagctgtgcc cggttcggcg gccacggctc
     2521 ggcgctggcc caggcctaca accacatgat catgccgctg gcctcggcgc gcgatcgcgc
     2581 gacccaggtg cggtggggca tcgccgactt ccagcgccgg ttcggccgcg cgccggaggg
     2641 catgtggctg cccgagtgcg cggtcgacac cccgtcgctc gaggcgctgg ccgccgaggg
     2701 catcgcgttc gcggtgctcg cgccgcacca ggccaaggcg tggcgcccgc cgggcggtgc
     2761 gtggcggacc cagccgatcg acaccggccg cgcctaccgc tgcgcgctgc cgtcgggccg
     2821 gaccatcgac ctgttcttct acgacggcgc gacctcgcgg gcggtcgcgt tcgagcgcct
     2881 gctcgccgac ggccaccaga tcatcgcgcg gatgaccgcg cgcggcgcgg tcgagggcgg
     2941 cggccccacg ctgtgtcaca tcgcgaccga cggcgagacc tacggccacc accaccgcta
     3001 cggcgacatg gcgctggcgt gggcgctgca gcaggtcgag cgcggctggg acggcacccg
     3061 gctgaccaac tacgccgagt tccgcgcccg cgtgccggcg acctgggagg tcgcgatcgc
     3121 cgagcacacg tcgtggagct gcgtccacgg caccgcgcgg tggcgcgacg actgcggctg
     3181 caacaccggc cgcgcgggct ggaaccagcg ctggcgccgg ccgctgcgcg acaccttcga
     3241 ctggctgcgc gaccaggccg cggcggcgct cgacaacgtc ggccggatcc tgttccgcga
     3301 tccatgggcc gcgcgcgacg cctacatcgc ggtggtgctc gagcgctccg ccgccgcgcg
     3361 cgatcagttc ctcgccgcgc acaccgcgca ccggctcgat cccgacgagc gcgtccgcgc
     3421 gctgtcgctg atggagatgg cgcgccacgc gatgctcatg tacaccagct gcggctggtt
     3481 cttcgacgac ctgtcgggga tcgagaccgt gcagtgcctg cagtacgccg cgcgggtcgc
     3541 cgagctgctc gaggacgtcg gcggcgcgcc ggtcgagccc gagctggtcg accggctgtc
     3601 cgcggcgagc tccaacctcg ccgaggaggg cgacggccgc caggtgttca cccggcacgt
     3661 ccagccggcg cgggtcgatc ccgccaaggt gtgcgcccac gtcgcggtcc aggcgctggt
     3721 cgagcccgag ggccagcaca gcgtcgacgt ctacggccac cacgtcgact tcctcgaccg
     3781 gatcgagcgg cgctccggcc acacccggat ggtcgccggc accgtccgcg tgcggtcgcg
     3841 gctgaccgag atcgcgacgt ccttgtgctt cgccggcctc cacctcggcg agcagcacgt
     3901 caccggcggc gtccgcccgc cgccgccgca gggcgcgtgg gccgcgatcg tcgaagagct
     3961 gaccggcgcg ttccgcaccg ccgacgtgtt cgccgcccag cgcgcgatcg cgcgccactt
     4021 cccgggccac gagctctctc tgtcatcgct gctccccggc agccgcgagc gcgtgctcgg
     4081 cgcggtgctc g
//
LOCUS       DASVOR010002221         2192 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_491814,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002221 DASVOR010000000
VERSION     DASVOR010002221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491814"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1178
                     /locus_tag="VF516_41155"
     CDS             <1..1178
                     /locus_tag="VF516_41155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEX8114230.1"
                     /translation="RDRLHAAAPGGRPMTRRVLILAAEVKQYRQSFLGELAARLRRDD
                     IALRIAYSDPNRVERVKSDNVELPPELGIKVPGVWLLGHRVFVQLAWAAIRDADMVIV
                     EQANKHALNYLLLALSRLGRKRVAYWGHGYNHQERTPGASEWLKRKLVTRVDWWFAYT
                     AGVGRYLAAQGVDPGIITVVQNTIDTRELADAVRACGPDDARALRRRLGIADHARVGL
                     FCGSLYADKKLDLLVAATQRIRERRPDFELIIVGDGPARDAIHAAAARLPFVHYVGPA
                     FGRDRAAYFAISDVFLIPGLVGLAIVDAFTVGLPVFATDIPGSGPEIEYLEPEVNGVM
                     TAHDAGAYAAAVCRVLDDPDQVARMRAAARATAARLTLAHMVEAFATGITRCLEARA"
     gene            complement(1222..2115)
                     /locus_tag="VF516_41160"
     CDS             complement(1222..2115)
                     /locus_tag="VF516_41160"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8114231.1"
                     /translation="MATVSDDSRVGPASLAHAFWLSIARASASFTAARWRGVRFLAYH
                     SVASEDELPGLSRRTLVLSEDGFRNHLAWIRRHGYVVVSMADARRLLRNGDAARAQFV
                     CLTFDDGRLDNFTVAWPLLQRAGYSAHFFVNSAVVGGSFEHPLEGGGVTRSGTERFMD
                     AEALRAVVREGGSIGSHAHHHTDLTTLGEPEILRELVESRRALEAWTGGPIDTHAYPY
                     AQYDARVLRATEAAGYTLAFNVNTGTVLAIGPHNRFALPRNVIRTGADNPDNYAIIRG
                     GFDFTRFYTAARQRWKSGPAS"
BASE COUNT          313 a          823 c          754 g          302 t
ORIGIN      
        1 atcgagatcg gctccacgcc gcagctcctg gaggccggcc gatgacccgg cgcgtcctga
       61 tcctcgcggc ggaggtcaag caataccggc agagcttcct cggcgagctc gccgcgcggc
      121 tccgccgcga cgacatcgcc ctccggatcg cgtacagcga tcccaaccgg gtcgagcggg
      181 tgaagtccga caacgtcgag ctcccccccg agctcgggat caaggtcccc ggggtgtggc
      241 tcctcgggca ccgcgtgttc gtccagctcg cgtgggcggc gatccgcgac gccgacatgg
      301 tcatcgtcga gcaggccaac aagcacgcgc tcaactacct gctgctcgcg ctgtcgcggc
      361 tgggccgcaa gcgcgtggcc tactggggcc acggctacaa ccaccaggag cgtacccccg
      421 gcgcgtcgga gtggctcaag cgcaagctgg tcacgcgcgt cgactggtgg ttcgcgtaca
      481 ccgcgggggt cggccgctac ctcgccgccc agggcgtgga ccccggcatc atcacggtgg
      541 tccagaacac gatcgacacc cgcgagctcg ccgatgcggt gcgcgcctgc ggccccgacg
      601 acgcacgcgc gctccgccgc cggctcggca tcgccgacca cgcgcgcgtc ggcctgttct
      661 gcggcagcct ctacgccgac aagaagctcg atctgctcgt cgcggcgacc cagcggatcc
      721 gcgagcgccg cccggacttc gagctgatca tcgtcggcga cggtccggcc cgcgacgcga
      781 tccacgcggc cgccgcccgg ctcccgttcg tccactacgt gggcccggcg ttcggccgcg
      841 atcgcgccgc gtacttcgcg atcagcgacg tgttcctcat ccccggcctc gtcgggctcg
      901 cgatcgtgga cgcattcacc gtcgggctcc cggtgttcgc gaccgacatc cccggatcag
      961 gtccggagat cgagtacctc gagccggagg tcaacggcgt gatgaccgcc cacgacgccg
     1021 gcgcctacgc cgccgccgtg tgtcgcgtgc tcgatgatcc cgaccaggtc gcccgcatgc
     1081 gcgccgccgc gcgcgcgacc gccgcgcgcc tcacgctcgc ccacatggtc gaggcgttcg
     1141 cgaccggcat cacgcggtgc ctggaggcac gcgcctgaag cgaggcggac gcgaggcgga
     1201 cgcgaggcgg acccgaggcc gtcagctcgc cggtccagac ttccagcgct gccgggccgc
     1261 ggtgtagaac cgcgtgaagt cgaacccgcc gcggatgatg gcgtagttgt ccgggttgtc
     1321 ggcgccggtc cggatcacgt tgcgcggcag cgcgaagcgg ttgtgcggcc cgatcgcgag
     1381 caccgtcccg gtgttcacgt tgaacgccag cgtgtacccc gcggcctcgg tcgcgcgcag
     1441 gacccgcgca tcgtactgcg catacgggta ggcgtgcgtg tcgatcggtc ctccggtcca
     1501 cgcctccagc gcgcggcggg attcgacgag ctctcgcagg atctccggtt cgccgagcgt
     1561 ggtgaggtcc gtgtggtgat gggcgtgaga cccgatcgat ccgccctcgc gcaccaccgc
     1621 ccgcagcgcc tccgcgtcca tgaaccgctc ggtgccggac cgggtcacgc caccgccctc
     1681 gagcgggtgc tcgaacgacc cgccgaccac ggcggagttg acgaagaagt gcgccgagta
     1741 gccggcgcgc tggagcagcg gccacgcgac ggtgaagttg tccagccgcc cgtcgtcgaa
     1801 cgtcaggcac acgaactgcg cgcgcgcggc gtccccgttt cgcagcagcc gccgcgcgtc
     1861 ggccatcgac acgacgacgt agccgtggcg gcggatccat gccaggtggt tgcgaaaccc
     1921 gtcctcgctc agcaccagcg tcctccgcga caggcccggc agctcgtcct cggaggcgac
     1981 gctgtgatag gccaggaagc gcacgccgcg ccagcgcgcg gcggtgaacg acgcgctggc
     2041 ccgggcgatc gagagccaga acgcgtgtgc cagcgaggcc gggccaaccc tggagtcatc
     2101 ggacacggta gccacggcct cctcctccgg tcgcagtgta catcgtgcag tccgggagtc
     2161 cacgagtcat ggctccgcac ccccggcgcc cg
//
LOCUS       DASVOR010002222         1520 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_112665,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002222 DASVOR010000000
VERSION     DASVOR010002222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112665"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /gene="greA"
                     /locus_tag="VF516_41165"
     CDS             complement(<1..458)
                     /gene="greA"
                     /locus_tag="VF516_41165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEX8114232.1"
                     /translation="MKAQLHKLKNVDRLANSRAIEVARGHGDLSENADYSAAKEEQGL
                     IEAKIRELEAKLTLAEVIDPTKLSGTRVKFGATVTIEDSDSGETQTYTIVGEHEADIK
                     RGRISIGAPVARAMIGKEVGDTVIVQSPKGRREYEVTEVEWLEVPPLEEVP"
     gene            complement(583..1191)
                     /locus_tag="VF516_41170"
     CDS             complement(583..1191)
                     /locus_tag="VF516_41170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114233.1"
                     /translation="MKAAVLVVLAAAGVAALAVASCSVHRLSDAYACDPSGNGSECGS
                     GRSCVQGFCVESGTTDGCPSQCTTCDSERSCRIECTPGNPCGNVQCPAGYDCKIRCNN
                     GGACGAVDCAQAHSCNVECSGSASCGNLNCGMHECTIQCSGALACPMIDCAASCSCDV
                     SCTGAAACPSMACPMGILGPCTQNGSAGAPCDSTEPGCGVCI"
     gene            complement(1193..>1520)
                     /locus_tag="VF516_41175"
     CDS             complement(1193..>1520)
                     /locus_tag="VF516_41175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114234.1"
                     /translation="GRTLGIALAAGGGGSLVLGLVLWVSYASVQDSIDHHATRTPADF
                     QDLTSLENRANTYAIAGNVFVLAGLVAGGIGGYLLYRDHQQHAVTVAPAPVAGGGMMI
                     VLTGGL"
BASE COUNT          212 a          542 c          505 g          261 t
ORIGIN      
        1 ggtacctctt ccagcggggg gacctcgagc cactcgacct cggtgacctc gtactcgcgc
       61 cggcccttgg ggctctgcac gatcacggtg tcgccgacct ccttgccgat catcgcgcgc
      121 gccaccggcg cgccgatcga gatccgaccg cgcttgatgt cggcttcatg ctcgccgacg
      181 atcgtgtagg tctgggtctc gccgctgtcg gagtcctcga tggtcacggt tgccccgaac
      241 ttgactctgg tacccgacaa tttggttgga tcgatgacct ccgcgagcgt caattttgcc
      301 tccagctcgc ggatcttggc ctcgatcagc ccctgctcct ccttggcggc gctgtagtcg
      361 gcgttctccg acaggtcgcc gtgccctcgt gcgacctcga tcgctcggct gttggcgagc
      421 cgatcgacgt tcttcagctt gtgcagctgc gccttcatcg aagcatgccc acccggggtc
      481 atggggtact tttcgctcat cgccgtcctc gtcgcggtcg tattggtagc cttgcgccgc
      541 caccagcgca agcaagtcca agagtttacc tcaggtcgcc gactagatgc acacgccgca
      601 cccgggctcg gtggaatcgc atggggcgcc ggcgctgccg ttctgggtgc acggccccag
      661 gatgcccatg gggcacgcca tgctcggaca ggccgccgcg ccggtacagg acacatcgca
      721 gctgcacgac gcggcgcagt cgatcatcgg gcacgcgagc gcccccgagc actggatggt
      781 gcactcgtgc atgccgcagt tgaggttgcc gcacgaggcc gagccggagc actcgacgtt
      841 gcagctgtgc gcctgcgcgc aatcgaccgc gccgcacgct ccgccgttgt tgcatcggat
      901 cttgcagtca tagcccgccg ggcactgcac gttgccgcac ggattgcccg gggtgcactc
      961 gatccggcag ctccgctcgc tgtcgcacgt cgtgcactgg ctcgggcagc catcggtggt
     1021 tccgctctcg acgcagaacc cctggacgca ggaccgcccg ctgccgcact cgctgccgtt
     1081 gccggacggg tcgcaggcgt aggcgtcgga cagccggtgc accgagcagc tcgcgaccgc
     1141 cagcgcggcg accccggcgg ccgcgagcac gacgaggacg gctgccttca tgtcacagac
     1201 ctccggtcag gacgatcatc atgccgccgc cggcgaccgg cgcgggcgcg accgtcaccg
     1261 cgtgttgctg gtgatcgcgg tagagcaggt agccgccgat cccgccggcg accagcccgg
     1321 cgaggacgaa cacgttgccc gcgatcgcgt aggtgttggc ccggttctcg agcgacgtca
     1381 ggtcctggaa atcggcgggc gtgcgggtcg cgtggtggtc gatcgagtcc tgcacgctgg
     1441 cgtagctcac ccacagcacg aggccgagca cgagcgagcc gccgccgccg gcggccagcg
     1501 cgatgccgag cgtgcggccc
//
LOCUS       DASVOR010002223         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_25354,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002223 DASVOR010000000
VERSION     DASVOR010002223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25354"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="VF516_41180"
     CDS             complement(<1..1006)
                     /locus_tag="VF516_41180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114235.1"
                     /translation="MQIGRAPRIVVLSPDPAFEHQLMTGLSAMACAVDVQRGPGALGA
                     GEPPAVLCVVHLEGDCARWLEARGSGRDPDCPVIAVLPRGDVAAAVDVLRASDRVAGV
                     IAADRFDSQQLVAQLVAQLVALATRIVTDDVFGLAPAMAPGTPIHARSVADHAEASRC
                     VALISELAEQCGLPRGCREPIAQCADEMLANALYDAPIDAQGRPLFQGVSPRARIALR
                     SEHSAAVQYACDGKRFAISVRDVFGTLERQTVVRTLHKCAHAERPIDRKVEGAGLGLY
                     LMVHAATAVFFHVLPGMATEVLCWFDVDAPGRAFAQLGFLTQRDAGVHRASGPAHRRL
                     A"
     gene            complement(1018..>1637)
                     /locus_tag="VF516_41185"
     CDS             complement(1018..>1637)
                     /locus_tag="VF516_41185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114236.1"
                     /translation="VALVNRLLAPNPDDRPQRGQDVAAELADISRQYGLSGSAPGIAY
                     VLLQLFPSEMAGGFEPQGTGRGVVRFVAEEGSGSVTKLEKSPSSVTHGSGSNSSSRRL
                     APLDVSETYRRRTGELSIPDVSELSTSPPPLVPAGDAASPAVEPWASHEARDPVTPLP
                     SRPSWTDSVRTEPIPRVRARSVTLALIAFPIAVALALVAYFLYGS"
BASE COUNT          253 a          613 c          582 g          189 t
ORIGIN      
        1 cggcgagccg ccggtgcgct ggcccgctcg cgcggtgaac gcccgcatcg cgctgcgtga
       61 ggaagccgag ctgcgcgaac gcgcggcccg gcgcatcgac gtcgaaccag cacagcacct
      121 cggtggccat gcccggcagc acgtggaaga acaccgccgt cgccgcgtga accatcaggt
      181 agagccccag ccctgccccc tcgaccttgc gatcgatcgg gcgctcggcg tgagcgcact
      241 tgtgcagggt gcggacgacg gtctggcgct ccagggtccc gaagacgtcg cgcacggaga
      301 tcgcgaagcg tttgccgtcg cacgcgtact ggaccgcggc gctgtgctcg ctgcgcagcg
      361 cgatccgcgc ccgcggcgag accccctgaa acagcggcct gccctgcgca tcgatcggcg
      421 cgtcgtacag tgcgttcgcc agcatctcgt cggcgcactg cgcgatcggc tcgcggcagc
      481 cgcgcggcag gccgcactgc tcggcgagct ccgagatcag cgccacgcac cgcgacgcct
      541 cggcgtggtc ggcgacgctc cgcgcgtgga tcggggtgcc cggcgccatc gccggcgcga
      601 gcccgaacac gtcgtcggtg acgatccggg tggccagcgc gaccagctgc gcgaccagct
      661 gcgcgaccag ctgctgcgaa tcgaagcgat ccgcggcgat cacaccggcg acgcgatccg
      721 aagctcgcag gacgtcgacg gcggccgcga cgtccccgcg cggcaacacg gcgatcaccg
      781 gacagtccgg gtccctgccc gacccgcgag cttccagcca gcgagcgcag tcgccctcga
      841 ggtggaccac gcacagcacg gcaggaggct cgcccgcgcc gagcgcgccg gggccgcgct
      901 ggacatcgac ggcgcacgcc atggcggaca gcccggtcat cagctggtgc tcgaaggccg
      961 gatccggcga caggaccacg atccgcggcg cacgcccgat ctgcatgcgt cgtgtaccta
     1021 gctcccgtac aggaagtagg ccacgagcgc cagcgcaacg gcgatcggga aggcgatcag
     1081 ggccagggtg acgctgcggg ctcgcacgcg aggaatcggc tcggtccgga ccgaatcggt
     1141 ccaggagggt cgagacggca acggcgtcac gggatcgcgc gcctcgtggg aagcccacgg
     1201 ctcgaccgcc ggcgaagcgg cgtctcccgc gggcaccagc ggcggcggcg aggtcgagag
     1261 ctcggacacg tccggaatcg acagctctcc ggtccgccgc cggtaggtct ccgacacgtc
     1321 cagcggcgcg agccggcggc tggacgagtt cgaacccgag ccgtgcgtga ccgagctcgg
     1381 cgacttctcc agcttcgtga ccgacccgga cccctcctcc gcgacgaacc ggacaacccc
     1441 cctgccggtc ccctggggct cgaacccgcc tgccatctcg ctggggaaca gctgcaagag
     1501 gacgtaggcg atgcccggcg ccgagcccga cagcccgtac tgccgggaga tgtcggccag
     1561 ctcggcggcc acgtcctgcc cgcgctgcgg ccggtcgtcg gggttgggcg ccagcagccg
     1621 gttcaccagc gccacca
//
LOCUS       DASVOR010002224         1653 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_82636,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002224 DASVOR010000000
VERSION     DASVOR010002224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82636"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1649
                     /locus_tag="VF516_41190"
     CDS             141..1649
                     /locus_tag="VF516_41190"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEX8114237.1"
                     /translation="MTGSTESFDVPLIIAGAETAAESGRTLPIINPATGRAIGTLAAA
                     GPADVDRAVQAAERAFDGGAWSQASVHHRARVLQRFADLFEADLEQFYSLETLNNGRP
                     IRETRAQIARLPQFYRYFAALALTRRSDVIPVEGPYLVYTQRVPLGPCALMSSFNHPL
                     MILSKSLAPALAAGNSVVIKPSEQTPITTLRLVRLLARAGVPDGVVNLINGEGREAGA
                     ALAQHPRIRKIVFTGGTEVGRSIAAAAAQRFALTTLELGGKGAVIVFDDIALDRAVNG
                     AAFAAFIAAGQTCVCGARILVQRSIYDRFLARFRAKAEAIRVGDPADPRTQLGPVISA
                     RSRDRILAMLERAQQAGARLLTGGGRAPVPGDGYFLQPTALYDVDPASEIAQQEVFGP
                     VTVILPFDDEADAIRIANATQFGLAASVWTIDVARAHRVASQLVFGMVWINDHHRLDP
                     ASPWGGFKDSGVGRETGIESFDQFTEPRAVTVNTSGETVDWYDDANPPKRLN"
BASE COUNT          223 a          640 c          570 g          220 t
ORIGIN      
        1 gctgcagatc gcggcggtgg cgggctgggg catcgagacc cggggccgga cgctcgagga
       61 cgtcgccgcc gatgcgacgc gccggccgct gccgctcgcg accgacgtca ccgcgcgctg
      121 aggcgcgcac cggaggcgcc atgaccggat ccaccgagag cttcgatgtg ccgctgatca
      181 tcgccggcgc cgagaccgcc gccgagagcg gccgcacgct gccgatcatc aaccccgcca
      241 ccggccgcgc gatcggcacg ctcgccgccg ccggccccgc cgacgtcgac cgcgccgtgc
      301 aggccgccga gcgcgcgttc gacggcggcg cgtggagcca ggcgtcggtc caccaccgcg
      361 ctcgcgtgct ccagcggttc gccgacctgt tcgaggccga cctcgagcag ttctactcgc
      421 tggagaccct gaacaacggc cggccgatcc gcgagacccg ggcgcagatc gcgcggctgc
      481 cgcagttcta ccgctacttc gccgcgctcg cgctgacccg gcgcagcgac gtcatcccgg
      541 tcgaggggcc gtacctggtc tacacccagc gcgtcccgct cgggccgtgc gcgctgatga
      601 gctcgttcaa ccacccgctg atgatcctgt ccaagagcct cgcgccggcg ctcgcggccg
      661 gcaactcggt ggtgatcaag ccgtcggagc agaccccgat caccacgctg cgcctggtcc
      721 gcctgctcgc ccgggccggc gttcccgacg gcgtggtcaa cctgatcaac ggcgagggcc
      781 gcgaggccgg ggccgcgctc gcccagcatc cccggatccg caagatcgtg ttcaccggcg
      841 gcaccgaggt cggccgctcg atcgccgcgg cggcggcgca gcgcttcgcg ctcaccacgc
      901 tcgagctcgg cggcaagggc gcggtgatcg tgttcgacga catcgcgctc gaccgcgcgg
      961 tcaacggcgc ggcgttcgcc gcgttcatcg ccgccggcca gacctgcgtg tgcggtgcgc
     1021 gcatcctcgt gcagcgctcg atctacgacc gcttcctcgc ccggttccgc gccaaggccg
     1081 aggcgatccg ggtcggcgat cccgccgatc cgcggaccca gctcggcccg gtgatctccg
     1141 cgcgatcgcg cgaccgcatc ctcgcgatgc tcgagcgcgc gcagcaggcc ggtgcccggc
     1201 tgctcaccgg cggcgggcgc gccccggtcc ccggcgacgg ctacttcctg cagccgaccg
     1261 cgctgtacga cgtcgacccc gcctccgaga tcgcgcagca ggaagtgttc gggccggtga
     1321 cggtgatcct gccgttcgac gacgaggccg acgcgatccg gatcgccaac gccacgcagt
     1381 tcggcctcgc cgcctcggtg tggaccatcg acgtcgcccg ggcgcaccgg gtggcgagcc
     1441 agctcgtgtt cggcatggtg tggatcaacg atcaccaccg cctcgatccc gcgtcgccat
     1501 ggggcggctt caaggacagc ggcgtcggcc gcgagaccgg catcgagtcg ttcgatcagt
     1561 tcaccgagcc gcgcgcggtc acggtcaaca ccagcggcga gacggtcgac tggtacgacg
     1621 atgccaatcc gccgaagcgt ctgaactgac gcg
//
LOCUS       DASVOR010002225         2289 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_112688,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002225 DASVOR010000000
VERSION     DASVOR010002225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112688"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2289
                     /locus_tag="VF516_41195"
     CDS             <1..>2289
                     /locus_tag="VF516_41195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8114238.1"
                     /translation="GAGGIGIFDWDIASDRVYWSPEVYRLMGLAPDAMEPSSMAWNAA
                     LVEEDLDIGWRAFRDAVAARRERYDLEMRLRQAGGGSRWVRVSSQLSYDEHGGPVRIL
                     GTIVDIEDLKQAAEAREAERQQLLRLLEQVPAVVNFLRGPDLVIELAHPAWIAQLGGR
                     DVVGKPLLEAVPEVRDQPRYARLRRVYQTGVADVQREVHTWAEVDGRRLDTYWDSAHV
                     AVRDATGRIEGVMTFEVDVTSSVQARRELELARAEAARASAQLRDLIAQSPIAMAMLS
                     GPEHRYELANQRYHEMVDRDQLVGKTVLEAFPEVADSPVMAVFDGVYRTGQPFSTDEY
                     PVSLIRRGRPEDCFFQFNLTPVRDAEGAITGLMVTAVEVTEQVRARRALEHSEARFRR
                     MFEANMVGFLFGTVDGHVLDANDYVLELLGVSRDELPALELMAMTVPEDVPRSLAASA
                     QLRERGLFEPFEKRFVRRDGTIVSVVIASALLPGATDRTITFMLDISERKRFEDQLAK
                     LRSEAETASRAKDEFLAMLSHELRNPLAPIMTALQLMRLRGDHAVERERVVIERQAHH
                     LVRLVDDLLDVSRITRGKIDLHPEPIELADVVAKAIEMASPLIEQRRHQLDVAVAPRG
                     LMISVDPARFAQVIANVLTNAAKYTEKGGRIEIAAARAGACVELAVRDSGIGIDADML
                     PRVFELFVQERQALDRSQGGLGLGLSIVKGLVELHGGTVRADSPGQGRGTTITIRVPA
                     LAGAPAPDGSLRAPPRPPTAGD"
BASE COUNT          319 a          817 c          836 g          317 t
ORIGIN      
        1 gggcgctggt gggatcggca tcttcgactg ggacatcgcc agcgatcgcg tgtactggtc
       61 gcccgaggtc tatcgcctga tgggcctcgc gccggacgcg atggagccgt cctccatggc
      121 gtggaacgcc gcgctcgtcg aggaggatct cgacatcggc tggcgggcat tccgggacgc
      181 ggtcgccgcg cggcgcgagc gctacgacct cgagatgcgg ctgcgccagg ccggcggcgg
      241 cagccgctgg gtccgggtgt catcgcagct cagctacgac gagcacggcg ggccggtgcg
      301 gatcctcggc acgatcgtcg acatcgagga cctcaagcag gcggcggagg cccgcgaggc
      361 cgagcgccag cagctgctgc gcctgctgga gcaggtgccc gcggtcgtca acttcctgcg
      421 cggccccgac ctggtgatcg agctcgcgca cccggcgtgg atcgcgcagc tcggcgggcg
      481 ggatgtggtc ggcaagccgt tgctcgaggc ggtccccgag gttcgcgacc agccgcgcta
      541 cgcccggctg cgccgcgtct atcagaccgg cgtggccgac gtccagcgcg aggtccacac
      601 ctgggccgag gtcgacggcc gccggctcga cacctactgg gactcggcgc atgtcgcggt
      661 ccgcgacgcg acggggcgga tcgagggcgt gatgaccttc gaggtcgacg tcacgtcgag
      721 cgtgcaggcc cgccgcgagc tcgagctcgc gcgcgccgag gcggctcgcg cgtcggcgca
      781 gctccgcgat ctgatcgcgc agtcgccgat cgcgatggcg atgctgtccg gccccgagca
      841 ccgctacgag ctggccaacc agcgctatca cgagatggtc gaccgcgatc agctggtcgg
      901 caagaccgtg ctggaggcgt tccccgaggt cgccgactct ccggtgatgg cggtgttcga
      961 cggcgtgtac cggaccggac agccgttctc gaccgacgag tacccggtat cgctgatccg
     1021 acgcggccgg cccgaggact gcttcttcca gttcaacctg acgcccgtgc gcgacgccga
     1081 gggcgcgatc accggcctga tggtcaccgc ggtcgaggtc accgagcagg tccgcgcgcg
     1141 ccgggcgctg gagcacagcg aggccaggtt ccgccggatg ttcgaggcca acatggtcgg
     1201 cttcctgttc ggcaccgtgg acggccacgt gctcgacgcc aacgactacg tcctggagct
     1261 gctgggcgtg tcgcgcgacg agctcccggc gctcgagctg atggcgatga ccgtgcccga
     1321 ggacgtgccc cggtcgctgg cggccagcgc ccagctccgc gagcgcgggc tgttcgagcc
     1381 gttcgagaag cgcttcgtcc gccgcgacgg cacgatcgtg tcggtggtca tcgcgtcggc
     1441 gttgctgccc ggcgccaccg accgcacgat cacgttcatg ctcgacatct cggagcgcaa
     1501 gcggttcgag gaccagctcg ccaagctgcg cagcgaggcc gagaccgcga gccgggccaa
     1561 ggacgagttc ctggcgatgc tcagccacga gctgcgcaac ccgctcgccc cgatcatgac
     1621 cgcgctgcag ctgatgcggc tgcgcggcga ccacgcggtc gagcgcgagc gcgtcgtgat
     1681 cgagcgccag gcccaccacc tggtgcggct ggtcgacgac ctgctcgacg tctcgcggat
     1741 cacgcgcggc aagatcgacc tccaccccga gccgatcgag ctcgccgacg tcgtcgccaa
     1801 ggcgatcgag atggcgagcc cgctgatcga gcagcggcgg caccagctcg acgtcgcggt
     1861 cgccccgcgc gggctgatga tctcggtcga tccggcgcgg ttcgcccagg tgatcgcgaa
     1921 cgtgctgacc aacgcggcga agtacaccga gaagggcggc cggatcgaga tcgccgccgc
     1981 gcgcgccggc gcgtgcgtcg agctcgccgt gcgcgacagc ggcatcggga tcgacgccga
     2041 tatgctgccg cgcgtgttcg agctattcgt gcaggagcgc caggcgctcg atcgctcgca
     2101 gggcgggctc ggcctcgggc tcagcatcgt caagggcctg gtcgagctgc acggcggcac
     2161 cgtgcgcgcc gacagccccg gccagggccg cggcacgacg atcacgatcc gcgtccctgc
     2221 gctcgccggt gcgccggctc cggacggctc gctgcgcgcg ccgccccggc cgcccaccgc
     2281 cggcgacag
//
LOCUS       DASVOR010002226         1667 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_491919,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002226 DASVOR010000000
VERSION     DASVOR010002226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491919"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1667
                     /locus_tag="VF516_41200"
     CDS             <1..>1667
                     /locus_tag="VF516_41200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114239.1"
                     /translation="LAPSAPRTPEEIAARQREVAAAREQTAQRDRQLVEDVHNNTISE
                     GGQNHVAQVFDHLTVGGGFASVADEMSRPGRSPDDIVIGGANPWDGATSKFGQPAGDS
                     ELPSHQAGHGMKDTARDPSADFMAASDHADNVALNRNDAQIKTYDGRAGALEPGPQAD
                     WPQFARDGGATVRTLVVGPDGEARYFYSKGVDVAAGPGPNRRLPTSVMDEATFNAMMA
                     DRALGFGDQAFQPDTVRPGEIANIGPGASGAWVGEAAGSVRNPDGTRANDIEWIANRP
                     TVDGQITPEQKQRLTEINQEIANARASGDPDRIAAAQAELTRWTFEEAARNGNLPRNR
                     EPGGAFDPAMQKGYADTHPGAEDGPLSRRVTDGIAKVTYEPQVDGGPNRVKITLKDVG
                     PDGQPVVVWKDSLVLSIGQDARGPGGPVSLLEHYDGQLIPIYGPEEPGGFRPVVGVES
                     PDGSVRVLGAAATPRDVSNLLDPHVMTADEHQANLQTQAGQLDANSKGVVQGFVLAEK
                     HIEASHAELAHLLTMTPEEQRAARLGLPPPAPRPGDPMRGSTPPPAP"
BASE COUNT          271 a          659 c          578 g          159 t
ORIGIN      
        1 gctcgcgccg tcggcgccgc gcacgcccga ggagatcgcc gcgcgccagc gcgaggtcgc
       61 cgccgcgcgc gagcagaccg cgcagcgcga ccgccagctg gtcgaggacg tccacaacaa
      121 cacgatcagc gagggtggcc agaaccacgt cgcccaggtg ttcgaccacc tcacggtggg
      181 tggcgggttc gccagcgtcg ccgacgagat gtcgcgcccg ggccgctcgc ccgacgacat
      241 cgtcatcggc ggcgccaacc cgtgggacgg cgcgaccagc aagttcggcc agccggccgg
      301 cgactccgag ctgcccagcc accaggccgg ccacggcatg aaggacaccg cgcgcgatcc
      361 gagcgccgac ttcatggcgg cgagcgatca cgccgacaac gtcgcgctca accgcaacga
      421 cgcgcagatc aagacctacg acggccgcgc cggcgcgctc gagcccggcc cgcaggccga
      481 ctggccgcag ttcgccaggg acggcggcgc caccgtccgg acgctggtgg tcggccccga
      541 cggcgaggcg cgctacttct acagcaaggg cgtcgacgtc gcggccggcc ccggccccaa
      601 ccgccggctg ccgaccagcg tgatggacga ggccacgttc aacgcgatga tggccgaccg
      661 cgcgctcggc ttcggcgacc aggcgttcca gcccgacacc gtccgccccg gcgagatcgc
      721 caacatcggc ccgggcgcat ccggtgcctg ggtcggcgag gccgccggca gcgtacgcaa
      781 ccccgatggc acgcgcgcca acgacatcga gtggatcgcc aaccgcccca cggtcgacgg
      841 ccagatcacg ccggaacaga agcagcggct gaccgagatc aaccaggaga tcgccaacgc
      901 cagggccagc ggcgaccccg accggatcgc cgccgcccag gccgagctca cccggtggac
      961 gttcgaggag gcggcgcgca acggcaacct gccgcgcaac cgcgagcccg gcggcgcgtt
     1021 cgaccccgcg atgcagaagg ggtacgccga cacccacccc ggcgccgagg acggcccgct
     1081 cagccgccgc gtcaccgacg gcatcgccaa ggtcacctac gagccccagg tcgacggcgg
     1141 gccgaaccgg gtcaagatca cgctcaagga cgttggcccc gacggccagc cggtcgtggt
     1201 gtggaaggac tcgctggtgc tctcgatcgg ccaggacgcg cgcggccccg gcggcccggt
     1261 gtcgctgctc gaacactacg acggtcagct gatcccgatc tacggccccg aggagcccgg
     1321 cgggttccgc ccggtggtcg gcgtggagag ccccgacggc tcggtccgcg tgctcggcgc
     1381 cgcggccacc ccgcgcgacg tgtcgaacct gctcgacccg cacgtgatga cggcggacga
     1441 gcaccaggcc aacctgcaga cccaggccgg ccagctcgac gccaactcca agggcgtggt
     1501 ccagggcttc gtgctcgccg agaagcacat cgaggcgtcc cacgccgagc tcgcccatct
     1561 cctgaccatg accccggagg agcagcgcgc ggcgcgcctg ggcctgccgc cgccggcgcc
     1621 ccggcccggc gatccgatgc gcgggagcac gccaccgccg gcgcccc
//
LOCUS       DASVOR010002227         5060 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_344933,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002227 DASVOR010000000
VERSION     DASVOR010002227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5060
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344933"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1196
                     /locus_tag="VF516_41205"
     CDS             <1..1196
                     /locus_tag="VF516_41205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114240.1"
                     /translation="GPSTGSSGGPSGGAASGGAVQRYAFINGKQVRKTDSFVTGGMVD
                     FVTDVAVRDYLTVDEFKSHVAGATDYLGNLDDDDHTWLRFSPTGLNVVGEAHETAWSL
                     PHVLAAVGSKSFISEAIASEEPAAGSALKTAYDARIADRTKELGIEKEKDKTKFGAEP
                     LPPKIGYSMVRLRPYLNLDKGTKDTDQNVHKLTKGARPDDYPGQLFLSMLQMGWGYAK
                     DARIAVAQAHMAMAAVPPKLDALAQVTDSLYHTLDSYITGLPAHGWLGDTLADPANAK
                     LVAPLAQFVDALIDMLTEQAVSDPSSGLDAKQKKKFAGTTTGAEKEEMFLGWRNRGIQ
                     HAVKDAAARGVRYAGMGKFHLDPLNKAGLPANTQVFDLISTGKEGDAFLDHTRELAKG
                     AVKQR"
     gene            complement(1222..2364)
                     /locus_tag="VF516_41210"
     CDS             complement(1222..2364)
                     /locus_tag="VF516_41210"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8114241.1"
                     /translation="MQLALTEDQALLARTANEFIAAGGSIARLRKLRDSRDERGYALD
                     RLVRMAELGWTGIPVADADGGLGMGLAEVVLVTEAMGRGLAPEPYIPSIVLAGRAVAL
                     GGSAQHKADWLGPAIAGQKVLALAHARRRGRFDLTRAPVRATRVDVGFRMTGEATQVW
                     GGHLADAYVIAARTAGDDGDRSGIALFLVPAGTPGLTAVRQHRIDSLNVAQLVLDDLV
                     VGDDAELAAAGDGFGLLSRVIDEATVALTGEMLGGMSEALERTLGYLRERRQFGVPIG
                     SFQALKHRAARLYIELELSRSAVMAAARAVDARSADAPQLVSLAKAKLSDAYCLIANE
                     AIQLHGGIGMTDEHDIGFFLKRARASEMTFGDAAYHRARFAELGGY"
     gene            complement(2414..3628)
                     /locus_tag="VF516_41215"
     CDS             complement(2414..3628)
                     /locus_tag="VF516_41215"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8114242.1"
                     /translation="MTDVESFRAELVEFLERELPRQLGALPPASADFWGGRRPELPHP
                     NSRRYCDLMAERGLTAPMWPREYGGGGLDKERARILVAELRRLGLPLPLIGFGMSMIG
                     PTLLQFGTDEQKREHLPKIVRGEIRWAQGYSEPNAGSDLASLGASARIAGDEILINGQ
                     KIWTSYADKADWMFGIFRTDAGGKKQEGITFLLLDMAQPGVEVRPIKLISGASPFCET
                     FFTDARAHTRDIIHGVGNGWTVAKALLGHERTMIGDLFGAGGRGRPSTTANPLVELAR
                     DYIGEHEREPGGRIADPVLRDRVTQFTMEERCFALTMQRSADAAKAGHKPGPESSIFK
                     IYGTELNQRRQELMLALRGPQGVGWEGEGFDERELAQTRDWLRSRGNTIEGGTSEVQL
                     NIIAKHVLALPD"
     gene            3752..4624
                     /locus_tag="VF516_41220"
     CDS             3752..4624
                     /locus_tag="VF516_41220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8114243.1"
                     /translation="MSEAQARALPTPANHNRIEDELRRVVAARDEVLAIVSHDLRNPL
                     SAVQLSTRLLGELGLDERAHKHLEMIQRATRSMSRLIDDLLDAVSIDSGKLALDLRPV
                     PVAAVLTEVRERNEPLARDRGVELRVSCGAPPEALNATASNATVSNVAIVGDRPRVLQ
                     VFGNLIGNALTFCRAGDTMVIACQVAGDAVEFSIADTGPGIDPGLVPHLFEPYWCAPP
                     NIRRRIGLALYIAKGIVERHGGRIRVERSETGGGDPAGSAGGAGGKAPRGIDTGPGAG
                     TLFSFTLPKAPKAP"
     gene            complement(4593..>5060)
                     /locus_tag="VF516_41225"
     CDS             complement(4593..>5060)
                     /locus_tag="VF516_41225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114244.1"
                     /translation="DACGSAGLIVPILDAATRAELDRMIPSYGSSQNPVDATAQAIVQ
                     SGYAELARLIAASDVVDAVIMIVSARITASFERERERLAEVAQGSAKPIVLWSYTLPS
                     ADTVRLVSQAGYPLFTSMQSCARAVAAMAHYRAVRERLRAPRAVTEPSGPSGA"
BASE COUNT          737 a         1811 c         1801 g          711 t
ORIGIN      
        1 cgggcccatc caccggctcg tccggcggcc cgtccggtgg cgcagcctcg ggcggcgcgg
       61 tccagcgcta cgcgttcatc aacggcaagc aggtccggaa gaccgactcg ttcgtcaccg
      121 gcggcatggt cgatttcgtc accgacgtgg cggtccgcga ctacctgacg gtggacgagt
      181 tcaagagcca cgtcgccggg gcgaccgact acctgggcaa cctggatgac gacgatcaca
      241 cgtggctgcg gttcagcccg accggcctca acgtggtcgg cgaggcgcac gagaccgcgt
      301 ggtcgctccc gcacgtgctc gcggcggtcg gcagcaagag cttcatcagc gaggcgatcg
      361 catccgagga gcccgccgcc ggctccgcgc tgaagaccgc ctacgacgcc cgcatcgccg
      421 accgcaccaa ggagctcggg atcgagaagg agaaggacaa gaccaagttc ggggccgagc
      481 cgctgccgcc caagatcggt tattcgatgg tccggctgcg gccgtacctg aacctcgaca
      541 agggcaccaa ggacaccgac cagaacgtcc acaagctgac caagggcgcc agaccggacg
      601 attaccccgg ccagctgttc ctgagcatgc tgcagatggg ctggggctac gccaaggacg
      661 cacggatcgc ggtcgcgcag gcgcacatgg cgatggcggc ggttccgccc aagctcgacg
      721 cgctcgcgca ggtgaccgac tcgctctacc acacgctcga ctcctacatc accggtctgc
      781 cagcgcacgg ctggctcggc gacacgctgg ccgatcccgc caacgccaag ctcgtggcgc
      841 cgctcgccca gttcgtcgac gcgctgatcg acatgctgac cgagcaggcg gtcagcgatc
      901 cgagcagcgg gctcgatgcc aagcagaaga agaagttcgc cggcacgacg accggcgcag
      961 agaaggagga gatgttcctc ggctggcgca atcgcggtat ccagcacgcg gtcaaggacg
     1021 cggcggcgcg cggcgtgcgc tacgccggca tgggcaagtt ccacctcgat ccgctgaaca
     1081 aggcggggct gccggcgaac acccaggtgt tcgacctgat cagcacgggc aaggaggggg
     1141 acgcattcct cgaccacacc cgggagctcg cgaagggggc ggtcaagcag cggtgaaccg
     1201 gcgcgcaggc cgccggcgcg ctcagtaccc gccgagctcg gcgaaccgcg cgcggtggta
     1261 ggccgcgtcg ccgaacgtca tctccgaggc ccgggcgcgc ttgaggaaga acccgatgtc
     1321 gtgctcgtcg gtcatgccga tgccgccgtg cagctggatc gcctcgttgg cgatcaggca
     1381 gtacgcgtcg gacagcttgg ccttggcgag cgacaccagc tgcggcgcgt cggcgctccg
     1441 ggcgtcgacc gcgcgcgccg ccgccatcac cgccgagcgc gacagctcga gctcgatgta
     1501 cagccgcgcg gcgcggtgct tgagcgcctg gaagctgccg atcggcaccc cgaactgccg
     1561 gcgctcgcgc aggtagccca gcgtgcgctc gagcgcctcg gacatgccgc cgagcatctc
     1621 gccggtgagc gccacggtcg cctcgtcgat cacccgggac agcaggccga agccgtcgcc
     1681 ggcggccgcg agctcggcgt cgtcgccgac gaccaggtcg tcgagcacga gctgcgcgac
     1741 gttgagcgaa tcgatccggt gctggcgcac cgccgtgagc cccggcgtcc cggccgggac
     1801 caggaacagc gcgatgccgc tgcgatcgcc gtcgtcgccc gcggtgcgcg ccgcgatcac
     1861 gtaggcgtcg gcgaggtggc cgccccacac ctgggtggcc tcgccggtca tccggaaccc
     1921 gacgtcgacc cgggtggcgc gcaccggcgc gcgggtcagg tcgaaccggc cgcgccgccg
     1981 ggcgtgggcg agcgcgagga ccttctgtcc ggcgatcgcc ggacccagcc agtccgcttt
     2041 gtgctgcgcg ctgccgccca gtgcgacggc acgccccgcc agcacgatcg agggaatgta
     2101 cggctccgga gcgagcccgc ggcccatcgc ctcggtgacc agcacgacct cggcaagccc
     2161 catgcccagg ccgccgtcgg cgtccgcgac cgggatcccg gtccagccga gctcggccat
     2221 ccggacgagc cggtcgagcg catagccgcg ctcgtcgcgg ctgtcgcgca gcttgcgcag
     2281 ccgcgcgatg gacccgccgg cggcgatgaa ctcgttcgcg gtgcgcgcca ggagggcctg
     2341 gtcttcggtc agtgcgagct gcatggcttc gttgtcctcg tactcgtcct tgtcgtccgt
     2401 cggcccggtc cggtcagtcc ggcagggcga gcacgtgctt ggcgatgatg ttgagctgga
     2461 cctcgctggt cccgccctcg atcgtgttgc cgcgcgagcg cagccagtcg cgggtctggg
     2521 cgagctcgcg ctcgtcgaat ccctcgcctt cccagcccac gccctgcggc ccgcgcagcg
     2581 cgagcatcag ctcctggcgg cgctggttga gctcggtgcc gtagatcttg aagatcgacg
     2641 actccggccc cggcttgtgc ccggccttgg cggcgtcggc gctgcgctgc atggtcagcg
     2701 cgaagcagcg ctcctccatg gtgaactggg tgacccggtc gcgcagcacc ggatcggcga
     2761 tccggccgcc cggctcgcgc tcgtgctcgc cgatgtagtc gcgcgccagc tcgaccagcg
     2821 ggttggcggt ggtcgacggc cggccgcggc cacccgcgcc gaacaggtcg ccgatcatcg
     2881 tgcgctcgtg gccgagcagc gccttggcca cggtccagcc gttgccgacg ccgtggatga
     2941 tgtcgcgggt gtgcgcgcgc gcgtcggtga agaacgtctc gcagaacggc gacgcgccgc
     3001 tgatcagctt gatcggccgg acctcgacgc cgggctgcgc catgtcgagc aggaggaacg
     3061 tgatgccctc ctgcttcttg ccgccggcgt cggtgcggaa gatgccgaac atccagtcgg
     3121 ccttgtcggc gtagctggtc cagatcttct ggccgttgat gaggatctcg tcgccggcga
     3181 tccgcgccga cgccccgagc gacgcgagat cgctgccggc gttgggctcg gagtagccct
     3241 gcgcccagcg gatctcgccg cggacgatct tcggcaggtg ctcgcgcttc tgctcgtcgg
     3301 tgccgaactg cagcagcgtc gggccgatca tcgacatccc gaacccgatc agcgggagtg
     3361 gcaggccgag ccggcgcagc tcggcgacca ggatccgcgc gcgctccttg tcgagcccgc
     3421 cgccgccgta ctcccggggc cacatcggcg cggtcagtcc gcgctcggcc atcagatcgc
     3481 agtagcgccg ggaattcggg tgcgggagct ccggccggcg gccgccccag aagtcggcgc
     3541 tggccggtgg cagcgcgccg agctggcgcg gtagctcgcg ctccaaaaat tccaccagct
     3601 cggcacgaaa gctctcgacg tcggtcatgg tctctccctg tgcggccgcg cgcgggtgcg
     3661 tcagacctcc cgctcttcgc atatccatga cgggattgcc agcgccgcgc cccctgcgcg
     3721 acgtggcaca gaacttggaa cttcgtgagg catgagcgag gctcaagcgc gcgccctgcc
     3781 gacgccggcc aaccacaatc ggatcgagga cgagctgcgt cgcgtggtcg ccgcgcgcga
     3841 cgaggtgctc gcgatcgtct cacacgacct gcgcaatccg ctgagcgcgg tgcagctcag
     3901 cacgcggctg ctcggcgagc tcgggctcga cgagcgcgcc cacaagcacc tggagatgat
     3961 ccagcgcgcg acccggagca tgagccggtt gatcgacgac ctgctcgacg ccgtgagcat
     4021 cgactccggc aagctcgcac tcgacctccg gcccgtgccg gtggcggcgg tgctgaccga
     4081 ggtccgcgag cgcaacgagc cgctcgcccg cgatcgcggg gtcgagctgc gcgtgagctg
     4141 cggggccccg ccagaggctt tgaatgccac ggcttcgaat gccacggtct cgaatgtcgc
     4201 catcgtcggc gaccgcccgc gcgtgctcca ggtgtttggc aacctgatcg gcaacgcgct
     4261 cacgttctgc cgtgccggcg acaccatggt gatcgcgtgc caggtcgcgg gcgacgccgt
     4321 ggagttctcg atcgccgaca ccgggccggg catcgatccg gggctggtcc cgcacctgtt
     4381 cgagccgtac tggtgtgcgc cgccgaacat ccggcgccgg atcggcctgg cgctctacat
     4441 cgccaagggc atcgtcgagc gccacggcgg gcggatccgg gtcgagcgaa gcgagacggg
     4501 gggtggggac cctgccggct ccgccggcgg ggcggggggc aaagcccccc gtgggatcga
     4561 cacggggccc ggagcgggga ccttgttctc gttcacgctc ccgaaggccc cgaaggctcc
     4621 gtgaccgcgc gcggcgcgcg caggcgctcg cggaccgcgc ggtagtgcgc catcgccgcc
     4681 accgcgcgcg cgcagctctg catgctggtg aacagcggat agccggcctg gctgaccagc
     4741 cgcaccgtgt cggcgctcgg cagcgtgtac gaccacagga cgatcggctt ggcgctgccc
     4801 tgggcgacct ccgcgagccg ctctcgctcg cgctcgaagc tcgcggtgat ccgcgccgag
     4861 acgatcatga tcaccgcgtc gacgacgtcg gacgcggcga tcaggcgggc gagctcggcg
     4921 tagccggact ggacgatcgc ctgcgcggtg gcgtcgaccg ggttctgcga gctgccgtag
     4981 ctcgggatca tccggtcgag ctcggcgcgg gtcgcggcgt cgaggatcgg cacgatcagg
     5041 ccggccgagc cgcacgcgtc
//
LOCUS       DASVOR010002228         1876 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_462338,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002228 DASVOR010000000
VERSION     DASVOR010002228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462338"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            192..1382
                     /locus_tag="VF516_41230"
     CDS             192..1382
                     /locus_tag="VF516_41230"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8114245.1"
                     /translation="MDFDPPEAVRPLLDRIEKFVTDVVMPAEPTVMERGFGASVALLD
                     ELRARCKAARLWGPQLPHELGGLGLGLVEHGLVSERLGRSPLGHYVFGCQAPDAGNIE
                     ILHKYGTPEQKAKWLEPLAHGKIRSCFSMTEPENPGSNPTIMSCRAVRHGDDYVIDGH
                     KWFTSAADGSAFAIVMAVTNPDAAAHSRASMIIVPTDTPGFDRVRNIKIMGDEGSGYG
                     THAEIWYRGVRVPVANRLGPEGAGFLIAQERLGPGRIHHCMRWIGICERTFDTMCRHI
                     TRRKIDDDKTLATKQIAQAWVAEARAEIDAARLMVLHAAWTIDKKGFSLARDSVSLIK
                     FFVADIMMRVIDRAIQLHGALGITSDTILAHYYVHERGARIYDGPDEVHKMAVSKRIL
                     ARYA"
     gene            1379..>1876
                     /locus_tag="VF516_41235"
     CDS             1379..>1876
                     /locus_tag="VF516_41235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEX8114246.1"
                     /translation="MSTLEDVPGTIRPGEELDIGKLSAYLDKQFGPGAVSVSQFPGGH
                     SNLTYLVRHGEPEYVLRRPPFGSKVKSAHDMGREYTVLTKLWPVYDKAPRPYAYEPTG
                     EVLGAPFYLMERRRGVVLRRSVPPGLADDLPRVRRICEHLVDALVDLHAVDYSAAGLG
                     DFGKPV"
BASE COUNT          304 a          674 c          640 g          258 t
ORIGIN      
        1 ggtgcggacc agcatcgtga tccgcaacgc ctcgatcacc gagctccggt tccgcagcca
       61 gaacttcgac tggcagcccg accagctgtc gctggtcacc ttcaacctca cctcgcacct
      121 ccccgcggag ctgcagaccg agcgatgacg ccactgatcc ccccgatgcc ccccacgaaa
      181 ggcaggtagc gatggacttc gatccacccg aggccgtgcg gccgctgctc gatcgcatcg
      241 agaagttcgt gaccgacgtc gtgatgccgg cggagccgac cgtgatggag cgcgggttcg
      301 gcgccagcgt ggcgctgctc gacgagctgc gcgcgcggtg caaggcggcc aggctgtggg
      361 gcccgcagct gccgcacgag ctcggcgggc tcgggctggg gctggtcgag cacggcctgg
      421 tgtcggagcg gctcggccgc tcgccgctcg ggcactacgt gttcggctgc caggcgcccg
      481 acgccggcaa catcgagatc ctgcacaagt acggcacgcc cgagcagaag gcgaagtggc
      541 tcgagccgct cgcccacggc aagatccggt cgtgcttctc gatgaccgag cccgagaacc
      601 cggggtcgaa cccgacgatc atgtcgtgcc gggcggtgcg gcacggcgat gactacgtga
      661 tcgacggcca caagtggttc accagcgccg ccgacggctc ggcgttcgcg atcgtgatgg
      721 cggtgaccaa ccccgacgcg gcggcccaca gccgcgcgtc gatgatcatc gtgccgaccg
      781 acacgcccgg cttcgatcgc gtccgcaaca tcaagatcat gggcgacgag ggctcgggct
      841 acggcaccca cgccgagatc tggtaccgcg gcgtccgcgt cccggtcgcc aaccggctcg
      901 gccccgaggg cgccggcttc ctgatcgcgc aggagcggct cggcccgggc cggatccacc
      961 actgcatgcg gtggatcggg atctgcgagc gcacgttcga cacgatgtgc cgccacatca
     1021 cgcggcgcaa gatcgacgac gacaagacgc tcgcgaccaa gcagatcgcg caggcctggg
     1081 tcgccgaggc ccgcgccgag atcgacgccg cgcggctgat ggtcctccac gcggcgtgga
     1141 cgatcgacaa gaaggggttc tcgctcgccc gcgacagcgt gtcgctgatc aagttcttcg
     1201 tcgccgacat catgatgcgg gtgatcgacc gcgcgatcca gctgcacggc gcgctcggca
     1261 tcaccagcga cacgatcctc gcgcactact acgtgcacga gcgcggcgcg cggatctacg
     1321 atggccccga cgaggtccac aagatggcgg tgtccaagcg aatcctggcg aggtacgcat
     1381 gagcacgctc gaggacgtcc ccggaaccat ccggccgggc gaggagctcg acatcggcaa
     1441 gctgtcggcg tacctggaca agcagttcgg ccccggcgcg gtcagcgtgt cgcagttccc
     1501 cggcgggcac tcgaacctga cctacctggt gcgccacggc gaacccgagt acgtgctgcg
     1561 ccggccgccg ttcggcagca aggtcaagtc cgcccacgac atgggccgcg agtacaccgt
     1621 gctgaccaag ctgtggccgg tctacgacaa ggcgccccgg ccctacgcct acgagccgac
     1681 cggcgaggtg ctcggcgcgc cgttctacct gatggagcgc cggcgcggcg tggtcctgcg
     1741 caggtcggtg ccgcccgggc tcgccgacga cctgccgcgg gtccgccgga tctgcgagca
     1801 cctggtcgac gcgctggtcg acctccacgc ggtcgactac agcgccgccg gcctcggcga
     1861 cttcggcaag ccggtc
//
LOCUS       DASVOR010002229         1575 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_344971,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002229 DASVOR010000000
VERSION     DASVOR010002229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344971"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1171)
                     /locus_tag="VF516_41240"
     CDS             complement(119..1171)
                     /locus_tag="VF516_41240"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114247.1"
                     /translation="MVEGTQIGAYRIVKPLGAGGMSEVWVAEHALLGRRAAIKVLRSR
                     LSRQSDIVQRMFNEARIMTAIANPGIVQVFDFGFHVDGSAYIVMELLDGETLGERLSR
                     RGPLAVGEALHLVRQVASALRAAHDHGVVHRDLKPENIFLTRDPDVAGGVRAKLLDFG
                     IAKLIGDSAITTGEAVVLGTPPFMSPEQCRGEETIEQRSDVYSLGCVLFTMVTGRPPF
                     DAAGGAVLTMHQLEPTPIPSRRMPGLPAVVDHLVLRCMAKHPAERFTAGELSRAIAGL
                     LHAPWLEPGERSAAAPYAVPPCDTTAATQPMEPVRPSLVQRLQRTAMFAEVAVLAMVI
                     CGMAWLAMVLLSRFVA"
BASE COUNT          227 a          618 c          527 g          203 t
ORIGIN      
        1 catgccccgc ccgctgcgcc tgtcccgctg cccgccgtgg cccccgtcgc cccgcccgcc
       61 aagccgggcg gcagctgacc gccggtcgat cgtcggtcag gcatcggtcg ggcgccggtc
      121 aggccacgaa tcgcgacagc agcaccatcg ccagccacgc catgccgcag atcaccatgg
      181 cgagcaccgc gacctcggcg aacatggcgg tgcgctgcag ccgctgcacc agcgacggcc
      241 ggaccggctc catcggctgg gtcgcggccg tcgtgtcgca cggcggcacc gcgtacggcg
      301 ccgcggcgct gcgctcgccg ggctccagcc acggcgcgtg caacagccct gcgatcgcgc
      361 gcgacagctc gccagccgtg aatcgctcgg ccggatgctt cgccatgcag cgcagcacca
      421 ggtgatcgac caccgcgggc agccccggca tgcgccgcga cgggatcggc gtcggctcca
      481 gctggtgcat cgtgagcacc gcgcctcccg cggcgtcgaa cggcggccgt ccggtgacca
      541 tcgtgaacag cacgcagccc agcgagtaga cgtcggagcg ctgctcgatc gtctcctcgc
      601 cgcggcactg ctcgggcgac atgaacggcg gcgtgccgag cacgaccgcc tcgccggtcg
      661 tgatcgcgct gtcgccgatc agcttggcga tcccgaaatc gagcagcttg gcccggacac
      721 cgcccgcgac gtccggatcg cgagtgagga agatgttctc gggcttgaga tcgcgatgca
      781 ccacgccatg gtcgtgcgcc gcgcgcagcg cgctcgcgac ctggcgcacc aggtggagcg
      841 cctcgccgac cgcgagcggc ccgcgccgcg acagccgctc gccgagcgtc tcgccgtcga
      901 gcagctccat gacgatgtag gcgctgccgt cgacgtggaa cccgaagtcg aacacctgca
      961 cgatccccgg gttcgcgatc gcggtcatga tccgcgcctc gttgaacatc cgctgtacga
     1021 tgtcggactg gcgggacagc cgcgaccgca ggaccttgat cgcggcccgg cgcccgagca
     1081 gggcgtgctc tgccacccag acctcgctca tcccaccggc gcccagcggc ttcacgatcc
     1141 ggtaggcccc gatctgcgtg ccttccacca tccgtgtcgt cctcgggagg tgctgcgggt
     1201 tcatcaccat gcggggtact gcgcgtgtgc atcgtgacaa gttgcagtca gcgaaatcgg
     1261 agcaggagca cgccgcgtac cagccgccac gcgcccccgc ggacccgtcg ccgccggcca
     1321 agtccgcgat cggctgtccc ctggggcccg ccggctgcgc gcgtgcgcaa acgcatcggc
     1381 ccactcgacg cgcatgcgcg tgcacgctcg aaccgagcca ccgggttctg ccatgcgttg
     1441 ccgccacgga cgatccaccg gtcgccggct gcgcgacgcc gcgacgggca ggcgcggccg
     1501 accgctgcca ctgcaatgcg acgctccgac cgtcgtgacc cgggcgcacg cgcgcacgcc
     1561 gcaacccgac gcacc
//
LOCUS       DASVOR010002230         3571 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_199681,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002230 DASVOR010000000
VERSION     DASVOR010002230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199681"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..1128)
                     /gene="nadA"
                     /locus_tag="VF516_41245"
     CDS             complement(94..1128)
                     /gene="nadA"
                     /locus_tag="VF516_41245"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEX8114248.1"
                     /translation="MERHLPVIGSVPGSVPGSVGPATGSPAAPDADLGMTPEELEAEI
                     RRLKRERNAVILAHYYQEDELQDLADHVGDSLQLSQAAAKTQADVIAFCGVHFMAETA
                     KILNPGKIVVVPDLDAGCSLADGCPAPVFQRWLERYPGHTVVSYINCSAEVKALSDVI
                     CTSSNAVKIVKALGDQKIVFAPDRHLGAFVARQAARPDLVCWQGTCVVHETFSERRLL
                     ELMAQHPEAEVIAHPECHDGILRHAAHIASTSGLIDHAKASPKQAFIVATEAGILHKM
                     RAAAPGKTLIPAPPDDESCSCNLCPHMKRNTLEKLYLALRDLAPRVDVPEPVRVRALR
                     PIQRMLEMSV"
     gene            1372..2280
                     /locus_tag="VF516_41250"
     CDS             1372..2280
                     /locus_tag="VF516_41250"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114249.1"
                     /translation="MIEPNSRIGRYRVVRSLGRGGMAGVFEVEEDTTGQRFALKAPIV
                     DVNPSGDVTRRFAREANALRLLDHPNLVTAVDVFVEAGYLFLVMEKVEGPTLGQVLHR
                     EPAGGGKPVKSALGPRRALVMARQILDGAGHAHARHLVHRDLKPDNLLLLDMGGWERV
                     KIIDFGIVKLLGDAAAAFGASALTNNGLVVGTPAYMAPEQALGRQIDARTDLYAIGVI
                     LFEMLTGRLPFQHDDPLKLMAMHVKSRLPKLDDVTQHAEWVTPEMCALVDGALIKDPA
                     HRFPSAEVMIQALDDAFYSIDHLDHI"
     regulatory      2446..2560
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2550..>3571
                     /locus_tag="VF516_41255"
     CDS             2550..>3571
                     /locus_tag="VF516_41255"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEX8114250.1"
                     /translation="MTSHGNGNGHNGNGNGNGNGNGNGNGNGNGNGNGKRSLPQAGAD
                     PVANLPAIEGFPASEKVHVERDGLRVPVRRIHLDGGEPPFDVYDTSGPQGVDPHVGLP
                     RLRKPWIDARLAAEATGNGAQAGNRTQLHYARRGIVTEEMRFVAIRESVAPEFVRDEI
                     ARGRAILPANIHHPESEPMIIGRNFLVKINANIGNSAVTSTIGEEVDKLRWAVKWGAD
                     TVMDLSTGKQIHETREWILRNAPVPIGTVPIYQCLEKAGGDPAKLDIEMFLDTLIEQA
                     EQGVDYFTIHAGVRLAYIPYTANRVTGIVSRGGSIMAKWCLSHHKENFLYTEFERICE
                     VMKKYDV"
BASE COUNT          573 a         1208 c         1292 g          498 t
ORIGIN      
        1 cgccttgggc cggccgcagg cgccttgggc cgggccgcag gcgccttggg ccgggccgca
       61 ggtgccttgg gccgggccgc aggcccgcgc cggtcagacc gacatctcca gcatgcgctg
      121 gatcggccgc agcgcccgga cgcggaccgg ctcggggacg tcgacccgcg gcgccagatc
      181 gcgcagcgcg aggtacagct tctccagcgt gttgcgcttc atgtgcgggc acaggttgca
      241 cgagcagctc tcatcgtcgg gcggcgccgg gatcagcgtc ttgccgggcg cggccgcgcg
      301 catcttgtgc aggatgccgg cctcggtcgc gacgatgaac gcctgcttcg ggctggcctt
      361 ggcgtgatcg atcaggccgc tggtcgacgc gatgtgggcg gcgtggcgca ggatgccgtc
      421 gtggcactcg gggtgggcga tcacctcggc ctcggggtgc tgcgccatca gctcgagcag
      481 ccggcgctcg ctgaacgtct cgtggacgac gcaggtgccc tgccagcaca cgagatccgg
      541 ccgcgcggcc tgcctggcga cgaacgcgcc gaggtggcgg tccggggcga acacgatctt
      601 ctgatcgccg agcgccttga cgatcttgac cgcgttcgac gacgtgcaga tcacgtcgga
      661 cagcgccttc acctcggcgc tgcagttgat gtagctgacc acggtgtggc ccgggtagcg
      721 ctccagccag cgctggaaca cgggcgccgg gcaaccgtcg gcgagcgagc atccggcatc
      781 gaggtcgggg acgacgacga tcttgccggg gttcaggatc ttcgcggtct cggccatgaa
      841 gtggactccg cagaacgcga tgacgtcggc ctgggtcttc gcggcggcct gggacagctg
      901 gagcgagtca cccacgtggt cggcgaggtc ctgcagctcg tcctcctggt agtagtgagc
      961 gaggatcacg gcgttgcgct cgcgcttgag ccggcgaatc tcggcttcga gctcctcggg
     1021 ggtcatgccg agatcggcgt cgggcgcagc cgggctaccg gtggcggggc cgacggagcc
     1081 ggggacggag ccggggacgg agccgatgac gggaaggtga cgttccatgg tcgagtccta
     1141 ccacgctgag ggtgtatcgc ggacactgga cgttcggcgg gtgctgcgtg ggatgccgcc
     1201 ggcgcggcaa gcgtcgcacc ggatcagaag acggctgcag gtgaggggaa gcgcacaggc
     1261 gggccgggag cgccggcggg agcgcggcgg cgatccgctc acggtgcagc gagatcgctc
     1321 gcatgattcg gtcgaactta cgctaacgtc gggactgttg tgagcagcct ggtgatcgag
     1381 cccaacagcc ggatcggccg gtaccgcgtg gtccggtcgc tcggccgcgg cggcatggcc
     1441 ggggtgttcg aggtcgagga ggatacgacc ggccagcggt tcgcgctcaa ggcgccgatc
     1501 gtcgacgtca accccagcgg cgacgtcacc cggcggttcg cccgcgaggc caacgcgctg
     1561 cgcctgctcg atcaccccaa cctggtcacc gcggtcgacg tgttcgtcga ggccggttac
     1621 ctgttcctgg tcatggagaa ggtcgagggc ccgacgctcg gccaggtgct ccaccgcgag
     1681 ccggccggcg gcggcaagcc ggtcaagtcc gcgctcgggc cgcgccgcgc gctggtcatg
     1741 gcgcgccaga tcctcgacgg cgccggccat gcccacgcgc gccacctggt gcaccgcgac
     1801 ctcaagcccg acaacctcct gctgctcgac atgggcggct gggagcgggt gaagatcatc
     1861 gatttcggca tcgtcaagct gctcggcgac gcggcggcgg cgttcggcgc ctcggcgctg
     1921 accaacaacg ggctggtggt cggcacgccg gcgtacatgg cgccggagca ggcgctcggc
     1981 cgccagatcg acgcgcgcac cgacctctac gcgatcggcg tgatcctgtt cgagatgctc
     2041 accggccggc tgccgttcca gcacgacgat ccgctcaagc tgatggcgat gcacgtcaag
     2101 tcgcggctgc ccaagctcga cgacgtcacc cagcacgccg agtgggtgac tcccgagatg
     2161 tgcgcgctgg tcgacggcgc gctgatcaag gatccggcgc accgcttccc gagcgccgag
     2221 gtcatgatcc aggcgctgga cgacgcgttc tactcgatcg accacctcga tcacatctga
     2281 cgggcacatc cgaggggaca tcccgagata ccgggtcaga cccgggccgg cgctcggggc
     2341 ctcggcgcac ggcctcacca tcgcgtctgc ggaatctcgg cggtccaggc caggctggcg
     2401 ccccggaggt gactgcggta gggtccgcca ggacagaacg agcccaacac accgcggggg
     2461 agcttcgggc gcatgcggcg cccaggctga gaggcacgcg agtgcgaccc cagaacctga
     2521 cccgggtaat gccggcgtag ggaggactga tgacgagtca cggtaacggt aacggccaca
     2581 acggcaacgg caacggcaac ggcaacggca acggcaacgg caacggcaac ggcaacggca
     2641 acggcaacgg caagcgctcc cttccgcagg cgggcgccga tccggtggcc aacctgccgg
     2701 cgatcgaggg cttcccggcg tcggagaagg tccacgtcga gcgcgacggg ctgcgcgtgc
     2761 cggtccgccg gatccacctc gacggtggcg agccaccgtt cgatgtctac gacacctcgg
     2821 gcccgcaggg cgtcgacccc cacgtcgggc tgcccaggct gcgcaagccg tggatcgacg
     2881 cgcggctcgc cgccgaggcg accggcaacg gcgcccaggc cggcaaccgc acccagctgc
     2941 actacgcgcg gcgcggcatc gtcaccgagg agatgcggtt cgtcgcgatc cgcgagagcg
     3001 tcgcgcccga gttcgtgcgc gacgagatcg cgcgcggccg cgcgatcctg cccgccaaca
     3061 tccaccaccc cgagagcgag ccgatgatca tcggccgcaa cttcctggtg aagatcaacg
     3121 ccaacatcgg caactcggcg gtgacgtcga cgatcggcga ggaggtcgac aagctgcgct
     3181 gggcggtcaa gtggggcgcc gacaccgtga tggacctgtc gaccggcaag cagatccacg
     3241 agacccgcga gtggatcctc cgcaacgcgc cggtgccgat cggcacggtg ccgatctacc
     3301 agtgcctgga gaaggccggc ggcgatcccg ccaagctcga catcgagatg ttcctcgaca
     3361 cgctgatcga gcaggccgag cagggcgtcg actacttcac gatccacgcc ggcgtccggc
     3421 tggcgtacat cccgtacacc gcgaaccggg tcaccggcat cgtgtcgcgc ggcggctcga
     3481 tcatggccaa gtggtgcctg tcgcaccaca aggagaactt cctctacacc gagttcgagc
     3541 ggatctgcga ggtgatgaag aagtacgacg t
//
LOCUS       DASVOR010002231         3654 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_550767,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002231 DASVOR010000000
VERSION     DASVOR010002231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550767"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VF516_41260"
     CDS             <1..767
                     /locus_tag="VF516_41260"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX8114251.1"
                     /translation="SAPDPRQRMSRSLRSPRRAPGRSAGHSLGHSPGLAGHGDLGASL
                     VLIFPLLLAYEIGVLFAGQVNGADLVTRAVLAAAGSRAAYLALYAAVAVGFLIWIRHT
                     RRWDTLGFALAGPVLLEATLYALSLGALVTLIVDRLLGLGLTLPSVISAIGAGVHEEL
                     VFRLALIAGLVALVRHFGPRAPLGGRLAVGIAIAASSLVFAAAHHVGRLGEPWTAHVF
                     AFRAVAGAVFGAVFWFRSLAHAVYAHVLYDLLVAAS"
     gene            complement(777..1721)
                     /locus_tag="VF516_41265"
     CDS             complement(777..1721)
                     /locus_tag="VF516_41265"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8114252.1"
                     /translation="MAPTSRTSYAAGHFKLDIDGHKSTAFVKSIEGGWSRGNITDDSI
                     GPDGRRIKQITSRDIDPITVEFGLAGANDMLKWIQSSWKRKPEKRHGQITHADFDMRT
                     VFEHEFVNAYITETTFPALDGSSKESGYIKCKLQPEYVLTRAIDPPGAKISADITAKQ
                     KMWNTSAFQLEIDGLDEMKYVNKLDSFTITQDIQKLRTGKTEFFEVVPHDLKFPNLTG
                     TISLEYTKRLHDWHSRFIRSQDGQAIKDPDALKSGAINFLSPDRKQVIFRINLYQVGL
                     TYLGIEPSKANEDGIKRAKFELYVHEMELDGSSILGFA"
     gene            1950..3458
                     /locus_tag="VF516_41270"
     CDS             1950..3458
                     /locus_tag="VF516_41270"
                     /inference="COORDINATES: protein motif:HMM:NF018095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEX8114253.1"
                     /translation="MNWKAVFVVLMLGALAWSRPADAEKVKTNQTAKLYSRAGEQSPV
                     ILKLKSGQTMTVLAKDGRWIKVRVSGRTGWIPRSKVDLPEDDEEIARNTRRRPFVDGR
                     GTKRGFGGESGPDDRIGADATGDGDDAPRSSGKLAKNDAKNDARNDAKNDDDDRPARK
                     KSRGDDDDARPGKDDDGDDAKEATRPMAHVAKTTDIYNEPNAGSDASFTAGPKTALYV
                     VEEKGKWTFVENDEGDAGYVLTSKLDIDAPAGGPHTRMIDLRGRLGFAQVNQSVSTPG
                     GTPIPDNYSAGSSSVGVALGAEVVYPYPKRYWLGGELAYTYTTTLFGGIQNMGQTIGF
                     SFHNFNLRALGGYDLQNKYGMIAFGRLGYHYDSFQVSDVANFAVNTARIPNQIIQGPT
                     IGAGLAIPRLTDSLGLRAGMDLMLIGASLEQTKNLEDGTGPGAKAVYLGSALNYRWKP
                     KIDIQGTLDLGYTSISFDGKAPATSMRMHTGTGPSSGSDFNVTFAVGVSYAL"
     gene            complement(3474..>3654)
                     /locus_tag="VF516_41275"
     CDS             complement(3474..>3654)
                     /locus_tag="VF516_41275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114254.1"
                     /translation="VKPTHYKVICISMYTRDIEELEAKVAELKRRGWTKANKSQLIRL
                     ALAQVDLDKLATPRQ"
BASE COUNT          548 a         1265 c         1242 g          599 t
ORIGIN      
        1 cctccgcccc ggacccgcgg caacgtatga gccggagcct gcgctccccc cgccgcgcgc
       61 ctggccgctc ggccggtcac tccctcggtc actcccccgg cctcgccggc cacggcgacc
      121 tcggcgccag cctggtgctg atcttcccgc tgctgctcgc ctacgagatc ggcgtgctgt
      181 tcgccgggca ggtcaacggc gccgacctcg tgacccgcgc ggtgctcgcc gccgccggat
      241 cgcgcgccgc gtacctggcg ctctacgccg cggtcgcggt cggcttcctg atctggatcc
      301 gccacacccg gcggtgggac acgctcgggt tcgcgctcgc cggcccggtg ctgctcgagg
      361 ccacgctcta cgcgctcagc ctcggcgcgc tggtcacgtt gatcgtcgat cgcctgctcg
      421 gcctcgggct gaccctgccg tcggtgatct cggcgatcgg cgccggcgtc cacgaggagc
      481 tggtgttccg gctcgcgctg atcgccgggc tggtcgcgct ggttcgccac ttcggcccgc
      541 gcgcgccgct gggcggccgg ctcgccgtcg ggatcgcgat cgcggcgtcg tcgctggtgt
      601 tcgccgccgc gcaccacgtc gggcggctcg gcgagccgtg gaccgcccac gtgttcgcgt
      661 tccgcgccgt cgccggggcc gtgttcgggg cggtgttctg gttccgctcg ctggcccacg
      721 cggtctacgc ccacgtgctc tacgacctgc tggtcgccgc gagctgaggc cgcgcgctac
      781 gcgaagccga ggatcgacga gccgtcgagc tccatctcgt ggacgtagag ctcgaacttc
      841 gcgcgcttga tgccgtcttc gttggccttg gacggctcga tgccgaggta cgtcaggccg
      901 acctggtaca ggttgatccg gaagatcacc tgcttgcggt cgggcgacag gaagttgatc
      961 gcgcccgact tcagcgcatc ggggtccttg atggcctggc cgtcctggct ccggatgaag
     1021 cggctgtgcc agtcgtgcag ccgcttggtg tactcgagcg agatcgtgcc cgtgaggttc
     1081 gggaacttga ggtcgtgggg cacgacctcg aagaactcgg tcttgccggt gcgcagcttc
     1141 tggatgtcct gcgtgatcgt gaacgagtcg agcttgttca cgtacttcat ctcgtcgagc
     1201 ccatcgatct cgagctggaa cgccgaggtg ttccacatct tctgcttggc ggtgatatcg
     1261 gccgagatct tcgcgccggg cggatcgatc gcccgggtca gcacgtactc gggctgcagc
     1321 ttgcacttga tgtagccgct ctccttcgac gagccatcga gcgccgggaa cgtggtctcg
     1381 gtgatgtacg cgttgacgaa ctcgtgctcg aacaccgtcc gcatgtcgaa gtcggcgtgc
     1441 gtgatctggc cgtggcgctt ctccggcttg cgcttccacg acgactggat ccacttcagc
     1501 atgtcgttgg cgccggccag gccgaactcc accgtgatcg gatcgatgtc gcgcgaggtg
     1561 atctgcttga tccgccgccc gtcggggccg atcgagtcat cggtgatgtt cccgcggctc
     1621 catccacctt cgatcgactt cacgaaggcg gtcgacttgt ggccgtcgat atcgagcttg
     1681 aagtgcccgg ccgcgtagct cgtcctgctt gtgggtgcca tggggtcgcc tcagcttaac
     1741 gcagtccctt ttacggcggc catcgcatcg ggatcccctg ggctggagac cctggtcccc
     1801 ggacctcgtg aggccgccgc caccaccacg gcagcggcca agccggcaga aacaggaacg
     1861 attgtcgtgg gatcccggct ggcccggccg ccgtcgccgg caaactcggc atggagggtt
     1921 cggcacgggg cttgtagaat ggcccggcca tgaactggaa ggccgtcttc gtcgtcctga
     1981 tgctcggagc gctggcctgg tcgcggccgg ccgatgccga gaaggtcaag acgaaccaga
     2041 ccgcgaagct gtacagccgg gccggagagc agtcgccggt gatcctcaag ctcaagtcgg
     2101 gccagaccat gacggtcctg gccaaggacg ggcgctggat caaggtccgg gtgtcgggtc
     2161 gcaccggctg gatcccgcgc agcaaggtcg acctgccgga ggacgacgag gagatcgccc
     2221 gcaacacgcg gcgccggccg ttcgtcgacg gccggggcac caagcgcggg ttcggcggcg
     2281 agagcggccc ggacgaccgg atcggcgccg acgcgaccgg cgacggcgac gacgcgccgc
     2341 gctcgagcgg caagcttgcc aagaacgatg ccaagaacga tgccaggaac gacgccaaga
     2401 acgacgacga tgaccggccg gcgcgcaaga agtcgcgggg cgacgacgac gatgccaggc
     2461 cgggcaagga cgacgacggc gacgacgcca aggaagcgac gcgaccgatg gcccacgtcg
     2521 ccaagaccac cgacatctac aacgagccga acgccggcag cgacgcctcg ttcaccgccg
     2581 ggcccaagac cgcgctgtac gtcgtcgagg agaagggcaa gtggacgttc gtcgagaacg
     2641 acgagggcga cgccggctac gtgctgacct ccaagctcga catcgacgcg ccggccggcg
     2701 gcccgcacac ccggatgatc gacctgcgcg gccggctcgg gttcgcccag gtcaaccagt
     2761 cggtcagcac gccgggcggc acgccgatcc ccgacaatta cagcgccggc agctcgtcgg
     2821 tcggggtcgc gctcggcgcc gaggtggtgt acccgtaccc gaagcgctac tggctcggcg
     2881 gcgagctcgc ctacacatac accacgacgc tgttcggcgg gatccagaac atgggtcaga
     2941 ccatcgggtt ctcgttccac aacttcaacc tgcgcgcgct cggcggctac gacctccaga
     3001 acaagtacgg catgatcgcg ttcgggcggc tcggctacca ctacgactcg ttccaggtgt
     3061 ccgatgtggc caacttcgcc gtgaacaccg cgcggatccc caaccagatc atccagggcc
     3121 cgacgatcgg cgccgggctg gcgatcccgc ggctgaccga cagcctgggc ctgcgggccg
     3181 gcatggatct catgctgatc ggcgccagcc tggagcagac caagaacctc gaggacggca
     3241 ccggcccggg cgccaaggcg gtgtacctcg gcagcgccct gaactaccgc tggaagccga
     3301 agatcgacat ccagggcacg ctcgacctgg gctacacgtc gatcagcttc gacggcaagg
     3361 cgccggcgac ctcgatgcgc atgcacaccg gcaccggtcc gagctcgggc tcggacttca
     3421 acgtcacgtt cgcggtcggc gtgtcgtacg cgctgtgatc gcccggcgcc cgcctactgg
     3481 cgcggcgtgg ccagcttgtc gaggtcgacc tgcgccagcg cgagccggat cagctgcgac
     3541 ttgttcgcct tggtccagcc gcggcgcttg agctcggcga ccttggcctc gagctcctcg
     3601 atgtcgcggg tgtacatcga gatgcagatc accttgtagt gcgtcggctt gacc
//
LOCUS       DASVOR010002232         2258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_520550,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002232 DASVOR010000000
VERSION     DASVOR010002232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520550"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..518
                     /locus_tag="VF516_41280"
     CDS             <1..518
                     /locus_tag="VF516_41280"
                     /inference="COORDINATES: protein motif:HMM:NF014864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114255.1"
                     /translation="YRGRHDRAGGVMADLDDTRARRALIVELIRTRHIATQGDLRDLL
                     RARGHDVTQATLSRDLAKLGARRVALPSGGTAYEVDGFTARTGGDPQLAAMAHLVLGI
                     REGAAIVVIHTRPGAAPAVAALLDQARLDSIIGSIAGDDTIFVVPERRVTTVALARAL
                     RRLVWPEAQSR"
     gene            complement(506..937)
                     /locus_tag="VF516_41285"
     CDS             complement(506..937)
                     /locus_tag="VF516_41285"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEX8114256.1"
                     /translation="MKVTVAAVGRLKEPYLVAAEDEYRKRLRPYCTLAVHEAKDEAAL
                     LAALPAGAHLYAFDERGELLTSAAFAESVLGHEQQHGGGAPVVFAIGGPDGHGDALRG
                     QARRLIAFGRLTIAHRLVRILVLEQLYRGFKILRGEPYHRD"
     gene            complement(941..1453)
                     /gene="rsfS"
                     /locus_tag="VF516_41290"
     CDS             complement(941..1453)
                     /gene="rsfS"
                     /locus_tag="VF516_41290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEX8114257.1"
                     /translation="MTQTKNKRTKQSTSVKPSASATKGRPRSAAPEPPPRAPATTEGL
                     DGAMRALTLALEKKALEPVLLDVRELCSFCNYQLVVSGRSDRQVEAITDGITSGLKLD
                     GLRPLGAEGARSGQWSLLDYGDFVVHVFLHSAREHYDLEGLWNDAPRIPLEVPPDARL
                     PAGEAYDAAR"
     gene            complement(1470..>2258)
                     /locus_tag="VF516_41295"
     CDS             complement(1470..>2258)
                     /locus_tag="VF516_41295"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEX8114258.1"
                     /translation="NRALLAAVHDGLAAAGLPTAAAQLVPITDREAIADLVRLDDLID
                     LCIPRGGEGLIRYVAEHARVPVIKHYKGVCHIYVHAAADPERALAIIENAKVKRPGVC
                     NAVETGLVDRAIAASFVPRLVARLAPAVELRGDDTVRALGGDAVRPASEDDWYAEYLD
                     LILAVRVVDGLDAAIAHIERYGSSHTESIVTTDSEAGERFVRELDSSTVMINASTRFA
                     DGGELGLGAEIGISTTRLHAYGPMGAEGLTTTKFVVRGTGQVRS"
BASE COUNT          293 a          794 c          861 g          310 t
ORIGIN      
        1 gataccgggg tcggcacgat cgtgcgggcg gagtgatggc ggacctggac gacacccggg
       61 cgcggcgggc gctgatcgtc gagctgatcc ggacccgcca catcgcgacc cagggcgatc
      121 tgcgcgatct gctgcgcgcg cggggccacg acgtcaccca ggccacgctg tcgcgcgatc
      181 tcgcgaagct cggggcgcgg cgggtcgcgc tgccgagcgg cggcaccgcc tacgaggtcg
      241 atggcttcac cgcgcgcacc ggcggcgatc cgcagctcgc ggcgatggcg cacctggtgc
      301 tggggatccg cgagggcgcc gcgatcgtcg tgatccacac ccgccccggc gccgccccgg
      361 cggtcgccgc gctgctcgac caggcgcggc tcgacagcat catcggctcg atcgccggcg
      421 atgacacgat cttcgtggtc ccggagcgcc gggtcaccac ggtcgcgctg gcccgcgcgc
      481 tgcgccggct ggtatggccg gaagctcagt cgcggtgata cggctcgccg cgcaggatct
      541 tgaagccgcg gtagagctgc tcgaggacga ggatgcggac caggcggtgc gcgatggtga
      601 gccggccgaa cgcgatcagc cggcgcgcct ggccgcgcag cgcatcgccg tggccgtcgg
      661 ggccgccgat cgcgaacacg acgggggcgc cgccgccgtg ctgctgctcg tggccgagca
      721 cgctctcggc gaacgccgcg ctggtcagta gctcgccgcg ctcgtcgaac gcgtagaggt
      781 gggcgccggc gggcagcgcg gcgagcagcg cggcctcgtc cttggcctcg tggaccgcga
      841 gcgtgcagta cggccgcagc cgcttgcgat actcgtcctc ggcggcgacc aggtacggct
      901 ccttgagccg gccgaccgcg gcgacggtga ccttcatgga tcagcgggcg gcgtcgtagg
      961 cctcgccggc gggcagccgc gcgtcgggcg ggacctcgag cgggatccgc ggcgcgtcgt
     1021 tccacaggcc ctcgaggtcg tagtgctcgc gcgccgagtg caggaacacg tggacgacga
     1081 agtcgccgta gtcgagcagc gaccactggc cggaccgcgc gccctcggcg ccgagcgggc
     1141 gcaggccgtc gagcttgagc ccgctggtga tgccgtcggt gatcgcctcg acctggcgat
     1201 ccgagcgccc cgacaccacg agctggtaat tgcagaacga gcacagctcg cggacgtcga
     1261 gcagcaccgg ctcgagcgcc ttcttctcga gcgccagggt gagcgcgcgc atcgcgccgt
     1321 cgagcccctc ggtggtcgcg ggcgcgcggg gcggcggctc gggcgcggcc gatcgcggcc
     1381 tgcccttggt cgccgacgcc gagggcttga ccgaggtcga ctgcttggtt cgcttgttct
     1441 tcgtctgggt catctggctc caggcatggc taggaacgga cctgaccggt accgcgtacc
     1501 acgaacttgg tggtggtgag gccctcggcg cccatcggac cgtaggcgtg gagccgggtg
     1561 gtcgagatcc cgatctcggc gcccaggccg agctcgccgc cgtcggcgaa ccgggtcgag
     1621 gcgttgatca tcacggtcga cgagtcgagc tcgcggacga accgctcgcc ggcctcggag
     1681 tccgtcgtga cgatcgactc ggtgtggctc gatccgtagc gctcgatgtg cgcgatcgcc
     1741 gcgtcgaggc catcgaccac gcgcacggcg aggatcaggt cgaggtactc ggcgtaccag
     1801 tcgtcctcgc tcgccgggcg gaccgcatcg ccgccgagcg cgcgcaccgt gtcgtccccg
     1861 cgcagctcga cagcgggggc caggcgcgcg accagccgcg gcacgaacga cgccgcgatc
     1921 gcgcgatcga cgagcccggt ctccaccgcg ttgcacacgc cgggccgctt gaccttggcg
     1981 ttctcgatga tcgcgagcgc gcgctcgggg tcggccgcgg cgtggacgta gatgtggcag
     2041 acgcccttgt agtgcttgat caccgggacc cgggcgtgct cggcgacgta gcggatcagg
     2101 ccctcgccgc cgcgcgggat gcacaggtcg atcaggtcgt cgagccggac cagatcggcg
     2161 atcgcctcgc gatcggtgat cggcaccagc tgggcggccg cggtgggcag cccggcggcg
     2221 gccaggccgt cgtggaccgc ggccaggagc gcgcggtt
//
LOCUS       DASVOR010002233         1801 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_286066,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002233 DASVOR010000000
VERSION     DASVOR010002233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1801
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286066"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VF516_41300"
     CDS             <1..206
                     /locus_tag="VF516_41300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114259.1"
                     /translation="CDALADRRPTSALAQARRFLAALIRSMHEPGARAIDARREMQAL
                     APRLLAAYLERRPGARTPLNGQG"
     gene            237..443
                     /locus_tag="VF516_41305"
     CDS             237..443
                     /locus_tag="VF516_41305"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEX8114260.1"
                     /translation="MDIRELTVSEVAARLGQPGFFVFDNNGEGRWKRGHVPGAKNLNA
                     HTYTAGDLPADKTATLVFYCSGPG"
     gene            708..836
                     /locus_tag="VF516_41310"
     CDS             708..836
                     /locus_tag="VF516_41310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114261.1"
                     /translation="MKALSRLSKLLPSQFEQVLYVARIPHEQLPRPPLPGHFALSR"
     gene            1592..>1801
                     /locus_tag="VF516_41315"
     CDS             1592..>1801
                     /locus_tag="VF516_41315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114262.1"
                     /translation="MGRALDKHLLILVDVSPGADERKQRSLAELRTHAKEASQTLLDT
                     YHRADPADSFGRWLLSLTLWELRTDD"
BASE COUNT          311 a          622 c          588 g          280 t
ORIGIN      
        1 cgtgcgatgc cctcgcggac cgccggccca cgtcggcgct cgcgcaggcg cgccgcttcc
       61 tcgccgccct gatccggtcc atgcacgagc ctggcgcccg ggcgatcgat gcgcgccgcg
      121 agatgcaggc gctggcaccg cggctcctcg ctgcctacct ggagcgccgc ccgggagcgc
      181 gcaccccgct gaacggccag ggatgacgcc ggagcatcgg tggtaatctc gttgatatgg
      241 atatccgcga gctcaccgtc tcggaggttg ccgcgcggct cgggcagccc ggcttcttcg
      301 tgttcgacaa caacggcgag ggccgctgga agcgcggcca cgtccccggc gcgaagaacc
      361 tcaacgcgca tacctacacc gccggcgacc tgccggccga caagaccgcg acgctggtgt
      421 tctactgctc cgggcctggt tgacacgcct gccacgcagg cgccgggcgc gcccaggagt
      481 tcggctaccc caacgccttc gcgatgcccg agggcatcgc gggatgggag aaggcgaagc
      541 ttccgaccga gaccggatag ccgccacacg cgatcggcag gcacggcgcc acagcgttgc
      601 gatgtccggc cgagtcagct cggatcattc acaacggcgt ctcgaccaag tgatcccgtt
      661 cgctgcctcg cggtggtgag cggcgccgag gcatcggggc gtaccccgtg aaggccctgt
      721 cccggctatc gaagctcctg ccatcgcagt tcgagcaggt gctctatgtg gccagaattc
      781 cgcacgagca gctcccgcgg ccaccgctgc ccgggcactt cgcgctgtcg aggtgatgcg
      841 ccacgtcgag cagcagaatc tgctcgacca tctggctcgg atcctcgagc aggtcgtgaa
      901 cggcggcagc ggacaggccg gcccggaccc tcggtcaccc gcccacgtca ccaagcaccg
      961 acgaggaatc gctcgccact gtgcgcaagg cagcggaatc gcgctgaaac ggtctcggtt
     1021 ctgggcgacg gatccggatc gcatccgctc tgcctcgaga cggtgacccg cggcggcacg
     1081 acccggtaaa agagaaacct cacggtcgcg gcgacgtcac attcggcggt gtctgcatct
     1141 tgtacacatg aatcgcgagc cgaggtaccg ccaggacgga caaggcggcc ttggccccga
     1201 tgcgcgattc gatccgaatt ttcgcttttc atataaatcg atattgtgat tgaacaccga
     1261 tataacgcga ttctgagaga acattgttcc cgggggtatc atgcgcaaaa aaggtatcta
     1321 tgtatcggtt tgcatgagtc tcgctgcggc aggggtcctt ggctattact ggactcgccc
     1381 ggcaggacct gcgctccagg gggcagcggc cacgcagacc cgctccccga gcggcccgtc
     1441 gcttgccctg gcgaacgatg gccgcgcgga ccgcagctcc ccggctggga cggcaacgcc
     1501 cgagacagcg acagccagcg cgaacggacc cgaccgagcc acgctggccc cgggcgcgtc
     1561 gagctcgggc gagcgctttc cggatacgcc ggtgggccgc gcgctcgaca agcatttgct
     1621 gatcctcgtc gatgtcagcc cgggcgccga cgagcggaaa cagcgatctc ttgcggagct
     1681 tcgaacccac gcgaaggagg ccagccagac gctgctggac acctatcatc gagcggatcc
     1741 ggcggactcc ttcggtcgat ggctgctcag cctgaccttg tgggagctcc ggaccgatga
     1801 c
//
LOCUS       DASVOR010002234         1877 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_432717,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002234 DASVOR010000000
VERSION     DASVOR010002234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432717"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..>1877
                     /locus_tag="VF516_41320"
     CDS             52..>1877
                     /locus_tag="VF516_41320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114263.1"
                     /translation="MTAFLGELVGAPVSDEDGAGAATAALRAARLDAQLMSEQMRRAW
                     LDFLHAETAAHPVLLVLDDLHWGDFGTVRFIDAALRDRGKQPWMVLALARPEVFEVFP
                     RLWTERQTIQELRLKELGRKAGERLVRQVLGDDVGSDTVERLVKRADGNAFYLEELIR
                     AVAEGKDAALPETVLAMVETRLARLPFEARRVLRAASVFGEVCWESGVVRLLGGAMAA
                     TSVSTWLAKLVEQEVLVVRPDGRFPGERELAFRHALLREGIYATLTADDQRLAHRLAG
                     EWLDRHGEANPMVLAGHFERGGDGAGAAGHYLRAAQQAMHILDHRAALVRTALGLACA
                     PPADVRIALLGLRCEAAQAAQQVSLDEAEQLLRLAVPGSVPWGQAMIAYHAGMMLAGR
                     HEELLTSIAWLRDVTPAPGAAGWTSLALLAGVFYLDMFGQVAQATALEQAFRAIVRAR
                     GDHEPLARVWWNATVATRASYAHDDPWTALQHAAAIQDIFDAIGGELIFIAMQILHGM
                     NQWYLGALAPAAQTLEAIPVADSTMGIVSSLRRFMLSWLHADLGALPRARALATQLAE
                     SGRAHGNPLEDGRGRWALAEVLRREGDLDGADREVGIALAMT"
BASE COUNT          229 a          722 c          678 g          248 t
ORIGIN      
        1 cggctccagc agcgaatcgc cgggtgcgtc ccgccggccg accgcgatcg ggtgaccgcg
       61 ttcctcggcg agctcgtcgg tgcgccggtg tccgacgagg acggcgccgg cgcggcgacc
      121 gcggcgctgc gggccgcgcg cctcgacgcc cagctcatga gcgagcagat gcgccgggcc
      181 tggctggact tcctccacgc cgagaccgcc gcgcacccgg tcctgctcgt gctcgacgac
      241 ctgcactggg gcgacttcgg caccgtgcgc ttcatcgacg ccgcgctgcg cgatcgcggc
      301 aagcagccgt ggatggtgct cgccctcgcg cggcccgagg tgttcgaggt gttccccagg
      361 ctgtggacgg agcgccagac catccaggag ctccgcctca aggagctcgg ccgcaaggcc
      421 ggcgagcggc tggtccgcca ggtgctgggc gacgacgtcg gcagcgacac ggtcgagcgc
      481 ctggtcaagc gggccgacgg caacgcgttc tatctcgagg agctgatccg cgccgtcgcc
      541 gagggcaagg acgcggcgct gccggagacc gtgctcgcca tggtcgagac ccggctcgcg
      601 cggctcccgt tcgaggcccg ccgcgtgctg cgcgccgcca gcgtgttcgg cgaggtgtgc
      661 tgggagagcg gcgtggtccg gctgctcggc ggggccatgg cagcgacgtc ggtgtccacc
      721 tggctggcca agctcgtcga gcaggaggtg ctggtcgtgc ggcccgacgg ccggttcccc
      781 ggtgagcgcg agctcgcgtt ccgccacgca ctgctccgcg agggcatcta cgccaccctc
      841 accgccgacg accagcggct ggcgcaccgg ctcgccggcg agtggctcga ccgccacggc
      901 gaggccaacc cgatggtgct cgccggccac ttcgagcgcg gcggcgatgg cgccggcgcc
      961 gccggccact acctgcgcgc cgcacagcag gcgatgcaca tcctcgacca ccgggccgcc
     1021 ctcgtccgca ccgcgcttgg cctggcctgc gcacctccgg ccgacgtccg gatcgccctg
     1081 ctcggcctgc gctgcgaggc ggcccaggcc gcgcagcagg tctcgctcga cgaggccgag
     1141 cagctgctgc gcctggcggt gccgggctcc gtgccctggg gccaggcgat gatcgcgtac
     1201 cacgccggca tgatgctcgc cggccggcac gaggagctcc tgacatcgat cgcctggctg
     1261 cgcgacgtca ccccggctcc cggcgccgcc ggatggacgt cgctcgcgct gctcgccggc
     1321 gtgttctacc tcgacatgtt cgggcaggtc gcgcaggcca ccgcgctcga gcaggcgttc
     1381 cgcgcgatcg tccgcgcgcg cggcgaccac gagccgctcg cgcgcgtgtg gtggaacgcg
     1441 accgtcgcga cccgcgcatc ctacgcgcac gacgacccgt ggaccgccct gcagcacgcc
     1501 gcggcgatcc aggacatctt cgacgcgatc ggcggcgagc tcatcttcat cgccatgcag
     1561 atcctgcacg gcatgaacca gtggtatctc ggcgcgctcg cgcccgccgc gcagaccctc
     1621 gaggccatcc cggtcgccga cagcacgatg ggcatcgtga gctcgctgcg gcgcttcatg
     1681 ctgtcctggc tccacgccga cctcggcgcc cttccccggg cgcgcgccct ggcgacccag
     1741 ctcgccgagt ccggacgcgc gcacggcaac ccgctggagg acgggcgagg ccgctgggcc
     1801 ctcgccgagg tgctgcgccg cgagggcgat ctcgacggcg ccgaccgcga ggtcgggatc
     1861 gcgctcgcca tgacgat
//
LOCUS       DASVOR010002235         4307 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_258572,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002235 DASVOR010000000
VERSION     DASVOR010002235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258572"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..1977
                     /locus_tag="VF516_41325"
     CDS             106..1977
                     /locus_tag="VF516_41325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114264.1"
                     /translation="MARRGTHDDLRTVEVPVPPPSAEEFAEAPRTVIDDSRVFDGLDG
                     AAEPDPEAATDGSPDPAAGAEPADPIHVLPTQQISAPRRASSSISRLSAAPTATVSPV
                     EAMKLDEVARTRVFLQVVFALCAGGIIVALSTAGDHVAKVVVLVGSVGSAVGAAVVLG
                     LTRDPTRYEPSRLFVPALLIVFGSMSGVYYWGAGSPVAAMLVYGIYFFSLGADRRVTT
                     AMYALVAATHGVLGIGIMTGLLADHGVVRIGPLRTREQLAIVCIIEALYFVAYYTARV
                     SQRVTLDAMGKLEQAVRGNAQRDALLAEARAELDRALRIGGPGRFTDQVVGSFRLGVL
                     IGRGGMGEVYEALAVADRREAAVKLLHPGTLAEPIHVQRFLREAQIASRLDCPHVVRV
                     LEVGTTASEIPFLVMERLRGADLAAQLRRQRRLALPPTKVLVDQVAIGLEAARVAGIV
                     HRDLKPHNVFFAEDGGLRRWKILDFGVSKAGGSGTLTQGHVVGTPAYMAPEQARGEDV
                     DHRADVYSLAAILFRAVTGHPVFSGKDVPSTLYEVVYRVPTRPSALAELPPDVDRVLA
                     LGLAKDPRERFGFALELARWFSAAVAQGLDPDQRRRADDLIARYPWGASLGAGGAVG"
     gene            2090..3217
                     /locus_tag="VF516_41330"
     CDS             2090..3217
                     /locus_tag="VF516_41330"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114265.1"
                     /translation="MARVLILWNQIDDDVYEHFRRDNRRAPDWDPTLEIEPWETVEQE
                     IELIERALRDGGHDAYHINIRDSFENLLDAVRRDPPDCVVNLVEFFHDDPEQEHNVPA
                     LFELLNIPYTGNRPLALSLCQKKPQAKALLASCGLPTPRGIVIDALPVPRQIDLRYPL
                     IVKPAHDDASGGIDAGSVVHDRAQLEARVKHVLSEHTMSALVEEYIAGRELHCAILGT
                     EALPLYEMRFKGGVDDLGRPLPHIITYRAKWDPYSRDHYAVESKCPADDLEPELVARI
                     QDIALRAFRAVGCRDYARVDMRLDVATGQPYILEVNPNPDLADSCAFAQSVRASGRTY
                     GQAMNELVHYALERGKIQPKRYIGPTDTLLSEYLARRAATK"
     gene            3559..3735
                     /locus_tag="VF516_41335"
     CDS             3559..3735
                     /locus_tag="VF516_41335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114266.1"
                     /translation="MATALWMMGRAQALPEVADPAKDLGALEREVRAAARRRSVRARA
                     SVDARLGRKPRADE"
     gene            4043..>4307
                     /locus_tag="VF516_41340"
     CDS             4043..>4307
                     /locus_tag="VF516_41340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HEX8114267.1"
                     /translation="MASKWLLALLGGALIGSGSALLLLTHGQIAGISGIAGGLVRSAT
                     ADRVWRFAFLAGLAVAGIVASAVVPGAIGAPVRPLAAMAVAGLL"
BASE COUNT          589 a         1551 c         1515 g          652 t
ORIGIN      
        1 cccggccgcg ccacgaggcg gtaacgaggc ggtaacgagg cgatgacggc gcgtttttcg
       61 tcggcgcgct gcgaccgcgt tcgcgacgaa ggctatgctc gcgccgtggc gcgacgcggc
      121 acccacgacg atctgcgcac ggtggaggtc cccgtgccgc cgccgagcgc cgaggagttc
      181 gccgaggcgc cgcgcaccgt gatcgacgat tcccgggtgt tcgacggcct ggacggcgcg
      241 gcggagccgg acccggaggc ggcgaccgac ggctcgcccg accccgccgc cggcgcggag
      301 cccgccgacc cgatccacgt cctgccgacc cagcagatca gcgcgccgcg ccgggcgtcg
      361 agctcgatca gccggctgtc ggccgcgccg accgcgacgg tgtcgccggt cgaggcgatg
      421 aagctcgacg aggtcgcgcg gacccgggtg ttcctccagg tcgtgttcgc gctgtgcgcc
      481 gggggtatca tcgtcgcgct cagcacggcc ggcgaccacg tcgccaaggt cgtcgtgctg
      541 gtcggctcgg tcggctcggc ggtcggcgcg gcggtcgtgc tcgggctgac ccgcgatccg
      601 acccgctacg agccgtcccg gctgttcgtc ccggcgctgc tcatcgtgtt cggctcgatg
      661 tcgggcgtct actactgggg cgccggttcc ccggtcgccg ccatgctggt ctacgggatc
      721 tacttcttca gcctgggcgc cgaccgccgg gtcaccaccg cgatgtatgc cctggtcgcg
      781 gcgacccacg gcgtgctcgg gatcgggatc atgaccggcc tgctcgccga ccacggcgtg
      841 gtccggatcg gcccgctgcg gacccgcgag cagctcgcga tcgtgtgcat catcgaggcg
      901 ctgtacttcg tggcctacta caccgcgcgg gtcagccagc gggtcacgct cgacgcgatg
      961 ggcaagctgg agcaggccgt gcgcggcaac gcccagcgcg acgccctgct cgccgaggcc
     1021 cgcgccgagc tcgaccgcgc gctcaggatc ggcggcccgg gccggttcac cgatcaggtc
     1081 gtcggctcgt tccggctcgg cgtgctgatc ggccgcggcg ggatgggcga ggtctacgag
     1141 gccctcgcgg tcgccgaccg gcgcgaggcc gcggtcaagc tgcttcaccc cggcacgctg
     1201 gccgagccga tccacgtcca gcggttcctg cgcgaggccc agatcgcgtc gcggctggac
     1261 tgcccgcacg tcgtccgcgt gctcgaggtc ggcaccaccg cgagcgagat cccgttcctc
     1321 gtcatggaac ggctgcgcgg cgccgacctc gcggcgcagc tgcgccgcca gcgccggctc
     1381 gcgctgccgc cgaccaaggt cctggtcgac caggtcgcga tcggcctgga ggccgcccgg
     1441 gtcgccggca tcgtccaccg cgacctcaag ccccacaacg tgttcttcgc cgaggacggc
     1501 gggctcaggc gctggaagat cctcgacttc ggcgtgtcca aggccggcgg ttccggcacc
     1561 ctcacgcagg gccatgtggt cggcacaccg gcgtacatgg cacccgagca ggcgcgcggc
     1621 gaggacgtcg accaccgtgc cgacgtgtac tcgctcgccg ccatcctctt ccgcgcggtc
     1681 accggccacc cggtattcag cggcaaggac gttccgtcga cgctgtatga ggtcgtctac
     1741 cgcgtgccga cgcgcccgtc cgcgctcgcc gagctgccgc ccgacgtcga ccgcgtcctc
     1801 gcgctcggtc tggccaagga tccgcgcgag cgcttcgggt tcgcgctgga gctcgcccgg
     1861 tggttctccg ccgcggtcgc ccagggcctc gaccccgacc agcgccgccg ggcggacgat
     1921 ctgatcgcgc gctacccgtg gggcgcgtcg cttggcgcgg gcggtgcggt cggctagacc
     1981 agatccgaga ccgagatcgg atcgcgagat cgttcgctgc cgctgcggac gatcatcgac
     2041 atcgcgtgcg catgatggta cgtgtgaata cagcggctct cgattcggca tggcacgggt
     2101 cctcatcctc tggaatcaga tcgacgacga tgtgtacgag cactttcgtc gcgacaaccg
     2161 gcgcgcccct gactgggacc ccacgctgga gatcgagccg tgggagaccg tcgagcagga
     2221 gatcgagctg atcgagcgcg cgctgcgcga cggcggccac gacgcctacc acatcaacat
     2281 ccgcgacagc ttcgagaacc tgctcgacgc ggtccgccgt gacccgccgg actgtgtggt
     2341 caacctggtc gagttcttcc acgacgaccc agagcaggag cacaacgtcc ccgcgctgtt
     2401 cgagctgctc aacattccct acaccggcaa ccggccgctc gccctctcgc tgtgccagaa
     2461 gaagccccag gccaaggccc tgctggcgtc gtgtggtctg ccgaccccgc gcggcatcgt
     2521 gatcgatgcg ctgccggtgc cacgtcagat cgacctccgc tacccgctga tcgtcaagcc
     2581 ggcgcatgac gacgcgtcgg gcggcatcga tgccggctcg gtggtccacg accgcgcgca
     2641 gctcgaggcc cgggtcaagc acgtgctgtc ggagcacacc atgtccgcgc tggtcgagga
     2701 gtacatcgcc ggccgcgagc tgcactgcgc gatcctcggc accgaggcgc tgccgctgta
     2761 cgagatgcgg ttcaagggcg gggtcgacga cctgggccgg ccgctgcccc acatcatcac
     2821 ctaccgcgcc aagtgggacc cctacagccg cgaccactac gcggtcgaga gcaagtgccc
     2881 ggccgacgac ctcgagcccg agctcgtcgc ccggatccag gacatcgcgc tgcgcgcgtt
     2941 ccgcgcggtc ggctgccgcg actacgcccg ggtcgacatg cggctcgacg tcgccaccgg
     3001 ccagccgtac atcctcgagg tcaaccccaa ccccgacctc gccgacagct gtgcgttcgc
     3061 ccagtcggtc cgcgccagcg gccggaccta cggccaggcg atgaacgagc tggtccacta
     3121 cgctctcgag cgcggcaaga tccagcccaa gcgctacatc ggcccgaccg acaccctgct
     3181 ctccgagtac ctggcgcgcc gcgcggcgac caagtaacgc gggagccctt ggccagatcg
     3241 tgaccgatgc aggtccagtt ggggcgcgtg cgtgcgtctg caccgggcgc gtgggtagcc
     3301 gacgtcaggt gcagatagtt gcatagactt gatcgaaagg agcggcctag taacatctat
     3361 attatgccaa gggctccgga agcactctcc gcgctccccc ccgccgcggc ccaagcgctc
     3421 cgtcagctcg gagagaacct ggcgatcgca cgggtccggc ggcgcgagac gcagcgcgcg
     3481 tgggccaagc ggatcggcgt atcgatcccg acgttgatcc ggctggagca cggggatccg
     3541 ggggtaggga tcggcatcgt ggccacggcg ctgtggatga tgggccgcgc gcaggcgttg
     3601 cccgaggtcg ccgatccggc gaaggatctc ggggcgctgg agcgcgaggt gcgagctgcg
     3661 gcgaggcgcc ggagcgtgag ggcgcgtgca tcggtcgatg cgcggctcgg acgaaagccg
     3721 cgagccgatg agtagcaccg cccgcgcgcg ctatcgcgtc accgatcagc tgtacctctg
     3781 gctgctcacg gagccagacc agcctgtcct gatcggggag ctcaacatgg tgcgctccag
     3841 ccgcggcgtg tcgctgcggt acgcgccgtc gtgcttggct accagcagat gcgcgtcggt
     3901 gtggatcagg ccgactcgac gctggtcaac gcgatgtcga tgtgctcgtc ctttggccgc
     3961 atggggggcc gtcggtcgcg acgacggcag tcccgcgtgt atcggctggc gtactggcac
     4021 tcgagatgct tatcgttgcc gtatggcttc gaagtggctc ctcgcgttgc tcggcggtgc
     4081 gctcatcggc tcgggcagcg cgctgctcct cctgacccac ggtcagatcg ccggcatcag
     4141 cgggatcgcc ggtgggctgg tccggtccgc gacggcggat cgcgtctggc ggttcgcgtt
     4201 cctcgccggg ctcgcggtcg cgggcatcgt cgcgtcggcc gtcgtgcccg gcgcgatcgg
     4261 cgcgccggtg cgaccgctgg ccgcgatggc ggtcgccggg ctgctcg
//
LOCUS       DASVOR010002236         1938 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_112859,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002236 DASVOR010000000
VERSION     DASVOR010002236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112859"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1680
                     /locus_tag="VF516_41345"
     CDS             <1..1680
                     /locus_tag="VF516_41345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8114268.1"
                     /translation="RMSAVDPAAPPGPGPGPGPAPGTGPPEERPNLLLPQVERWPLDP
                     ARPVIELDDVWVAFGDKQVLRGLSLRILPGQTTVVVGRSGSGKSVLLRVMMGLQKPDR
                     GRVTLFGRDLATCTPVEVIELRKRMGMLFQNYALFDALTVDENVGFSLQENSRLPQRE
                     IAQLTAELVRILNLTGSELLLPAELSGGMRKRVSLARALIANPEVVLFDEPTTGLDPI
                     MIAQVDDMIALAKQEYQITSVIISHDMASTQRLADNVAFLHDGRIIFYGNYDAFMHSE
                     LPPIQAFVEGARTSRLSRTGDAEAPGPPAAAAITDPPVVELIDVYKTFGDKPVLKGVS
                     FHIYPRKITVIIGASGSGKSVIIKHIMGLFKPDRGQIRVFGQDIVPMKDRELNEMRKH
                     FGLLFQSAALLDWLSVYENVAFPLRERGGNLPRGELRDRVLDILERLNLNDISHRMPG
                     EISAGQRKRVGLARAIVMKPDIMIYDEPTTGQDPLRTRDIDDMIQQTQERFDITSIVI
                     SHDMASTFRIGHMITMLYQGRIVAFGSPAELRASRDPHVQRFIHAGSVDVH"
BASE COUNT          306 a          735 c          603 g          294 t
ORIGIN      
        1 cggatgtccg ccgtcgaccc cgccgcgccg cctggccccg gccccggccc cgggcctgcc
       61 cccggcaccg gcccgcccga ggagcgcccc aacctgctgc tgccccaggt cgagcgctgg
      121 ccgctcgatc cggcgcggcc ggtgatcgag ctcgacgacg tgtgggtcgc gttcggcgac
      181 aagcaggtgc tgcgcggcct gagcctccgg atcctccccg gccagaccac cgtcgtcgtc
      241 ggccgcagcg gctcgggcaa gtcggtgctg ctccgcgtga tgatgggcct gcagaagccg
      301 gaccgcggcc gcgtcaccct gttcggccgc gacctcgcga cctgcacgcc ggtcgaggtc
      361 atcgagctcc gcaagcggat gggcatgctg ttccagaact acgcgctgtt cgacgcgctc
      421 accgtcgacg agaacgtcgg gttctcgctc caggagaact cgcggctgcc gcagcgcgag
      481 atcgcccagc tcaccgctga gctggtccgc atcctcaacc tcaccggcag cgagctcctg
      541 ctccccgccg agctgtccgg cggcatgcgc aagcgggtca gcctggcccg cgccctgatc
      601 gccaaccccg aggtcgtgct gttcgacgaa ccgaccaccg gcctcgatcc gatcatgatc
      661 gcgcaggtcg acgacatgat cgcgctcgcc aagcaggagt accagatcac gtcggtcatc
      721 atcagccacg acatggccag cacccagcgc ctggccgaca acgtcgcgtt cctccacgac
      781 ggccggatca tcttctacgg caactacgac gcgttcatgc acagcgagct gccgccgatc
      841 caggcgttcg tcgagggcgc ccgcacctcg cggctgtcgc gcaccggcga cgcggaggcc
      901 cccgggccgc cggccgccgc ggcgatcacc gacccgccgg tcgtcgagct gatcgacgtc
      961 tacaagacgt tcggcgacaa gccggtgctc aagggcgtca gcttccacat ctacccgcgc
     1021 aagatcaccg tgatcatcgg cgcgtccggc tccggcaagt cggtcatcat caagcacatc
     1081 atgggcctgt tcaaacccga ccgcggccag atccgcgtgt tcggccagga catcgtcccg
     1141 atgaaggacc gcgagctcaa cgagatgcgc aagcacttcg gcctcctgtt ccagagcgcc
     1201 gccctgctcg actggctgtc ggtctacgag aacgtcgcgt tcccgctgcg cgagcgcggc
     1261 ggcaacctgc cgcgcggcga gctgcgcgac cgcgtgctcg acatcctcga gcgcctcaac
     1321 ctcaatgaca tctcgcaccg catgcccggc gagatctccg ccggccagcg caagcgcgtc
     1381 ggcctggccc gcgccatcgt catgaagccc gacatcatga tctacgacga gccgaccacc
     1441 ggccaggatc cgctgcgcac ccgcgacatc gacgacatga tccagcagac ccaggagcgg
     1501 ttcgacatca ccagcatcgt gatcagccac gacatggcct ccaccttccg gatcggccac
     1561 atgatcacca tgctctacca gggccggatc gtcgccttcg gctcacccgc cgagctccgc
     1621 gcctcgcgcg atccccacgt ccagcgcttc atccacgccg gcagcgtcga cgtccactag
     1681 gaacgtgtag cgcatagcgc ccgggagaac gctcacacgc gacctcgccg ggggctgctg
     1741 cgcggatggg tgtcggagcc gcgcccgccg aatcaccacc ggcccatgcg cggacggcgc
     1801 gactatgttg tggcggtcgt gtcaagcgca gatctccctt gtccggatgg tttcctccgg
     1861 tcggtcgggt tggctgcggt tccaggagtg gctggcgtcc gggttggagg tgtctggctg
     1921 tagcagtcga ctattcgc
//
LOCUS       DASVOR010002237         7152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_520570,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002237 DASVOR010000000
VERSION     DASVOR010002237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520570"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VF516_41350"
     CDS             <1..537
                     /locus_tag="VF516_41350"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX8114269.1"
                     /translation="CLVVARDRAAAAAIKDAFATKHRVRKQYLAVVHGQPPWDDEHTI
                     DLALRVAQPGDPTRLTHVRMLAGPGGLPAITRVRVERRTAAYALVRCTLVTGRQHQIR
                     AHLAATGYPIVGDKLYAHGDDAFLRYCNDGLVPELARLFVLPRHALHAAAVSFPHPDG
                     GTVTAEAPLPADLAGLLT"
     gene            723..1322
                     /locus_tag="VF516_41355"
     CDS             723..1322
                     /locus_tag="VF516_41355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114270.1"
                     /translation="MAASRLLVIALSLVALLHAPARADDDPPRAQPMPLPVYPAPLAQ
                     TTQSTYVPQSVAMSGPEEITDFDFSRPVPLGYTPVARPRKHLIVGGAVTFGVVYGLSA
                     FVASIGEDAAHNGGRNEVGSLWIPVAGPFLQMGQTDSATGRFFFASVGVAQLAGALML
                     YYGLTTKEHVLVRNDLVGSLTVTPMAGHGASGMALAGRF"
     gene            complement(1733..1975)
                     /locus_tag="VF516_41360"
     CDS             complement(1733..1975)
                     /locus_tag="VF516_41360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114271.1"
                     /translation="MKVLVIHNGRGKIEGLAVPGPAVVGQIQMVPRKGRQVTEVDVED
                     IRGDVLSSENQQRLLGIVEGCRLEFGPGGARLVPRG"
     gene            complement(2013..2288)
                     /locus_tag="VF516_41365"
     CDS             complement(2013..2288)
                     /locus_tag="VF516_41365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114272.1"
                     /translation="MPIKQVNSDLWLLRVGLRANTVEEWFQVQPFGVVRWFNALVLSG
                     RAGNKGAEVTRVFDVDNGAGLETHVVFRVLPPSPEGASIAIYFAETV"
     gene            complement(2496..2978)
                     /locus_tag="VF516_41370"
     CDS             complement(2496..2978)
                     /locus_tag="VF516_41370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114273.1"
                     /translation="MCELVCKLVCEQAALGDDVEAGQQPQPLVSHVRHDWLRCSIDHS
                     LSASIDRSACSARIVFQEFAIDAFDAVLVDDRLPESFRSRRYPQMQEFLQRRRKRWTA
                     KTGVCRDLGAQRRCDRLGCPHRAPRSGLIIPGLDEVLAGLPERGAAARRSQHRAILMT
                     "
     gene            complement(3238..3975)
                     /locus_tag="VF516_41375"
     CDS             complement(3238..3975)
                     /locus_tag="VF516_41375"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEX8114274.1"
                     /translation="MTVLGSPRTPRGTANGKALQFFPVEAGYQAVFAIALDARPDPVT
                     IKVDGVAEIQTVQVGEVTFPETDVVVEDELANPSKPDRERIDADNAAILRAVAKARGE
                     PQFVRAFRRPPGEINSVFGEWRTFNDGHRSQHLGLDLFARESSRVKAINAGTVVLVRP
                     CFLAGNVVVVAHGGGIASAYFHLSKVSVAEGDRVAQGDELGLAGHTGRTTGPHLHLSI
                     HVPGGLVDPVAFFKLTIPPNGATVTRR"
     gene            4556..5236
                     /locus_tag="VF516_41380"
     CDS             4556..5236
                     /locus_tag="VF516_41380"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8114275.1"
                     /translation="MPGVRDDLQLRSLLDRLHAQSLAQEGDIRAHFAEQGSASVVGTR
                     AELEQGRAFWRDKLVALDADKAQFCYALCRALHARRIVEAGTSFGISTLYLAAAVRDN
                     GGGIVIGAEQEPSKVAVARGHFREARLDGYIDLREGDILEALEGLEGPVDFLLLDIWT
                     PLARPVLERVAPHLRTGAIIATDNTARRRQEYGALLAYLGDPANGFITQTLPFDGGFE
                     ISVKVPGH"
     gene            complement(5264..6280)
                     /locus_tag="VF516_41385"
     CDS             complement(5264..6280)
                     /locus_tag="VF516_41385"
                     /inference="COORDINATES: protein motif:HMM:NF037061.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEX8114276.1"
                     /translation="MDEDWKNAIKAGWKAQKQFSQAADEAKTKIQRWRGPLIVLSMLG
                     AIAAVVSHEFSGWFGSQSQQGPVVIGLIGTLFLACAAYLTKEVLSPKLEQSWTSCRSI
                     SEALKADCYRFALGGAPFTADATGIKQFNDRVEELTKDTDFNLLSAVDVADTDLKPQR
                     KIDLAHYVKFRAEEQRDRLQQKGKELNDQDAKWRTRSIALALVSIALPAVGSTLKLPV
                     VTAVVPIVTTAIASLTSYVFAMRLHATASLYNATAFQLDRLLARPKDASFSEKCEAIL
                     LASNQAWMAEWSKQVEGGSQPATPISAATPISAATPISEAAPTSGAAPTSGAAPTSGA
                     GQGG"
     gene            complement(6414..>7152)
                     /locus_tag="VF516_41390"
     CDS             complement(6414..>7152)
                     /locus_tag="VF516_41390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114277.1"
                     /translation="SMAGRRLTFDTRTFDGSPRSYRNAPVPGARVAGELYPVALIAPA
                     SWLAGFGVAADYDRTPSLTLRASNEMTVPLAATERHYAFSVRYRLAFGHQPTSPTLTL
                     GAGYGARRFAVDRRGLQSPSSLDLPDVDYRMFDPGVAFRLPLGGHVAVALAGQGLIVT
                     SAGPIQRADQYGAARVLGGTASAGVELLITERLLIRLAAEATQLNLSFTGTGMLANSR
                     DGDPSTVDVHGATDRYYGGVATAAVTY"
BASE COUNT         1126 a         2486 c         2388 g         1152 t
ORIGIN      
        1 tgcctggtgg tcgcgcgcga tcgcgccgcc gccgccgcga tcaaggacgc gttcgccacc
       61 aagcaccgcg tgcgcaagca gtacctcgcg gtggtgcacg gccagccgcc gtgggacgac
      121 gagcacacga tcgatctggc gctgcgcgtc gcccagcccg gtgacccgac ccggctgacc
      181 cacgtgcgga tgctcgccgg cccgggcggc ctgcccgcga tcacccgcgt ccgggtcgag
      241 cgacggaccg cggcctacgc gctggtccgc tgcaccctgg tcaccgggcg ccagcaccag
      301 atccgcgccc acctcgcggc caccggctac ccgatcgtcg gcgacaagct gtacgcgcac
      361 ggcgacgacg cgttcctgcg ctactgcaac gacggcctgg tccccgagct ggcgcggctg
      421 ttcgtgctgc cgcgccacgc gctgcacgcc gccgccgtga gcttcccgca ccccgacggc
      481 ggcaccgtca ccgccgaggc gccgctgccg gccgacctcg cgggcctgct cacctgaggg
      541 tgccgcctcg gtgccgcgcg ccgctgcacc cgaccaccgg ccgccggctc cgggtggcaa
      601 aaacctgtcg cggaatgtga tagcgatgtc agaccttggc gccgtccacc ggatcggcga
      661 tcgtgaaacc catggattcc tgggcgcttg gtcgtggcac ggcaatggct atgacggcgg
      721 gcatggccgc atcccgactc cttgtgatcg ccctctcgct ggtcgccctg ctccacgccc
      781 ctgcccgggc cgacgatgat ccgccgcggg cccagccgat gccgctgccg gtgtacccgg
      841 cgccgctggc gcagaccacg cagtcgacct acgtgcccca gagcgtcgcg atgtccggcc
      901 cggaagagat caccgacttc gacttcagcc ggccggtgcc gctcggctac acgccggtcg
      961 cgcgcccccg caagcacctg atcgtcggcg gcgcggtcac gttcggcgtg gtctacggcc
     1021 tgtcggcgtt cgtcgcctcg atcggcgagg acgctgcgca caacggcggc cgcaacgagg
     1081 tcggctcgct gtggatcccg gtcgccggcc cgttcctcca gatgggccag accgacagcg
     1141 cgaccggccg gttcttcttc gccagcgtgg gcgtcgcgca gctcgccggc gcgctgatgc
     1201 tgtactacgg cctgaccacc aaggagcacg tgctggtccg caacgacctc gtcggcagcc
     1261 tgacggtcac gccgatggcc ggccacggcg cgtcgggcat ggcgctggcc ggccggttct
     1321 agcgccgctt cattcaccac cacgtagcga gagccatcgg cgcgagtccg atcgggccgc
     1381 actcacgccc aacggcgctc gaaccgatca tacggaatgc catgtgttcc gttggatccc
     1441 gtggcatcaa cgcgtcgcca ctccgattcg gacagcaacg gacgttgttg cttggaagat
     1501 gggatcccaa ccatgcctct cggcggaaac catacgtagg tgtccaaatc ggttcgcatc
     1561 tggctcatag atgctgagga tgctaactcg gccgcagccc gggcgacgtg gcgggcgagc
     1621 cccgagccgc gaactccggg ccggggcggt cacgtcagca ccgcagcgaa cgggcgaccc
     1681 gccgcggttc gtgctcagcg aggtcggggc tcccgtccgt ttgcgcttcc gctcagccgc
     1741 ggggcaccag ccgagcgcca ccggggccaa actccagtcg acatccctcg acgatcccga
     1801 ggagacgctg ctggttctcg ctgctgagca cgtcgccccg gatgtcctcg acgtcgacct
     1861 ctgtgacctg acggcccttg cgcggcacca tctggatctg ccccacgacc gccgggcccg
     1921 gcaccgccag gccctcgatc ttgccccgcc cgttgtgaat caccaagacc ttcatgttcg
     1981 tatccctctc tcgtcgccca ccatgcggcg gcctagacgg tttctgcgaa gtagatcgcg
     2041 atgctcgcac cctccggcga gggtggcagc acacggaaga cgacgtgcgt ctcgaggccg
     2101 gccccgttgt cgacgtcgaa cacacgggtc acctcggccc ccttgttacc cgcccgtccg
     2161 ctcaggacca gtgcattgaa ccaccgcacg accccgaagg gctgcacctg gaaccattcc
     2221 tcgacggtgt tcgcgcgcaa ccccacgcgg aggagccaaa ggtcgctgtt aacctgcttg
     2281 atcggcatgc acctctcctt cctcgtcgtg gcgaggaacc aacaagacac tccgtccgta
     2341 cctgacagtc ggatcatgcc ccatgcaagc actgcgccct ctgcatgcct ccaaggggat
     2401 ttgcactgca cggtcatgaa tcgagaaaat tcatgctgat gcgggatgcc acttctgtga
     2461 tgcgagcgcg cgccatctga catccaacct acacatcagg tcatcaatat tgcacgatgt
     2521 tgtgagcggc gtgctgcggc gccacgctct ggaagtccag cgaggacctc gtcgagaccg
     2581 ggtatgatca gaccgctgcg cggcgcacgg tgcggacaac ccaggcgatc gcagcggcgc
     2641 tgagcgccta ggtcacggca aacaccggtc ttggcggtcc aacgcttccg ccgacgttgc
     2701 aggaactcct gcatctgcgg atagcggcgc gaccggaagg attccggcag tcggtcatcg
     2761 acgagcacgg catcgaaggc gtcgatcgcg aactcttgga acacgatccg tgccgaacac
     2821 gcgctgcgat cgatactcgc actcaagctg tggtcgatcg agcaccgcag ccagtcatgc
     2881 cgaacgtggc tgacgagggg ctggggttgc tggccggcct caacgtcatc cccaagtgca
     2941 gcttgctcgc acacgagctt acatacgagc tcacacacgc ccgcaatcgc agcttgtccg
     3001 tggggggaag accgcgggaa gacctgtacg gggggggggg gggccggtga ggggtgccgg
     3061 tgcgttggga cgagtaccca ccctcagatc gtccggggtt tcaggcctcg atcaccgtgg
     3121 gcgatgttcg gtaacggcag ctaggtgcgc ggctgtcccg cgtaacgttg cagtgcacag
     3181 cgcgggcgct gctggcggag tagcgagcgg ttcgctccac gcggcgtggt cgacggatca
     3241 gcggcgcgtg accgtcgcgc cgttcggcgg aatcgtgagc ttgaagaacg ccacgggatc
     3301 gaccaggccg cccggaacgt ggatgctgag gtgcaggtgc ggtccggtgg tgcgcccggt
     3361 gtggccagcg aggccgagct cgtcgccttg cgcgacacgg tccccctcgg cgaccgagac
     3421 cttgctcagg tggaagtacg ctgatgcgat gccgccgcca tgcgcgacca cgaccacgtt
     3481 gccggcgagg aaacacgggc gcaccaggac aacggtgccg gcgttgatcg ccttgacccg
     3541 ggaactctcg cgcgcgaaca ggtcgagtcc gaggtgctgg ctgcggtggc catcgttgaa
     3601 cgtgcgccac tcaccgaaca ccgagttgat ctcgcccggc ggccggcgga acgcgcgcac
     3661 gaactgcggc tcgcccctgg ccttcgcgac ggcgcgcagg atcgcggcgt tgtccgcgtc
     3721 gatccgctcg cggtcgggct tcgacggatt ggcgagctcg tcctcgacca ccacgtcggt
     3781 ctcgggaaac gtcacctcgc cgacctgcac ggtctggatc tccgcaacgc cgtcgacctt
     3841 gatcgtcacc ggatcgggtc gggcatcgag cgcgatcgcg aacaccgcct gatagcctgc
     3901 ctcgaccgga aagaactgga gcgccttgcc gttggcggtg ccccgggggg ttcgcggact
     3961 gccgaggacc gtcacgagga ccggatcgcc ggggcgagcg gtcgtcgggg tgacacggag
     4021 cgtcgtgtcg gctgcagcgg tcgacgctgc ggccagcaca actccgaaca gcgcgcacgt
     4081 agttgttcgt gggctcatga tccggtgtat gcctatcgct cagaacggca agtgtgcgca
     4141 tcggcgctca ccgctgcgcg cggccgcaca cgacgcaagc ccatgagaac ccggcacacg
     4201 cgacccaagg ctactcggcg gggtgggcat gttctgcctc ggctggaacg tgacggtcag
     4261 accgccgtgg tacgcacccg gctgccagga gaccgtgtga ttcaagctcc gcgccttcaa
     4321 gactgaatct cgaatcgcgt ggggttggtc ggccatggcc aaacattccc gcgtagcgaa
     4381 ccagatgacg accgggtggc gggatgatcc cgcacagccg ccgtaggatc gccaccagca
     4441 gcagcaccag ctcggtgcgg ccgtcgggcc acggccgttc gccggcggac tttcggcaca
     4501 tggtgctgga gctgctcgaa cagatcccga tcggttctct gctacgctgc gcctcgtgcc
     4561 cggcgtacgc gacgatcttc agctccggtc gctgctcgac cgcctgcatg cacaaagcct
     4621 tgcgcaggag ggcgacattc gcgcccactt cgccgaacag ggaagcgcga gcgtggtcgg
     4681 cacccgtgcc gagctcgagc agggccgcgc gttctggcgc gataagctgg tcgccctcga
     4741 cgccgacaag gcgcaattct gctacgcgct gtgccgggcg ctccatgcgc ggcgcatcgt
     4801 cgaggctggt acgtcgttcg gcatctccac gctgtacctc gcggcggcgg tgcgcgacaa
     4861 cggcggcggc atcgtcatcg gcgccgagca ggagcccagc aaggtcgcgg tcgcccgcgg
     4921 acacttccgc gaggcgcgcc tcgatggcta catcgacctc cgcgagggcg acatcctcga
     4981 agccctcgag ggcctcgaag ggccggtgga cttcctgctg ctcgacatct ggacgccgct
     5041 tgcacgcccc gttctggagc gggtcgcgcc tcacctgaga accggcgcga tcatcgctac
     5101 cgacaacacg gccaggcggc gccaggagta cggcgcgctg ctcgcctacc tcggcgatcc
     5161 ggccaacggc ttcatcaccc agacgctgcc gttcgatggc ggcttcgaga tctcggtgaa
     5221 ggtgcccgga cattagcccg cggacaggcg ggtcacggca tcatcacccg ccttgtcctg
     5281 ccccgctggt cggtgctgcc ccgctggtcg gtgctgcccc gctggtcggt gctgcctcgc
     5341 tgatcggtgt tgccgcgctg atcggtgttg ccgcgctgat cggtgttgca ggctgggatc
     5401 cgccctctac ctgcttcgac cattcggcca tccacgcctg gttcgaggcc aggaggattg
     5461 cctcgcattt ctccgagaac gatgcgtcct tggggcgcgc cagcagccgg tccagctgga
     5521 atgcagtcgc gttgtacagc gacgcggttg cgtggagtcg catcgcaaac acgtagctgg
     5581 tcaacgaagc gatggcggtc gtgacgatcg gaacgaccgc cgtgacgacc ggcagcttca
     5641 gcgtggaacc cacggcgggg agcgcgatgg agacgagcgc cagggcgatg cttcgcgtgc
     5701 gccacttcgc gtcttgatcg ttcagctcct tccccttctg ttgaagacgg tcccgctgtt
     5761 cttcggcgcg aaacttgacg taatgcgcga gatcgatctt gcgttgtggc ttgagatcag
     5821 tgtctgccac gtctactgcg gacaggagat tgaaatcggt gtccttcgtg agctcctcga
     5881 cccgatcgtt gaactgcttg atcccggtgg catccgccgt gaacggcgcc ccaccgagcg
     5941 cgaaccgata acaatctgcc ttcagcgctt cggagatgga acgacagctc gtccatgatt
     6001 gttcgagctt gggagaaagg acttccttcg taagatacgc ggcacacgcc agaaacaggg
     6061 ttccgatcag accgatcacg accggaccct gctgggactg cgagccgaac cagccggaaa
     6121 actcgtggct gaccacggct gcaatggctc ccagcatgct gagaacgatc agagggccgc
     6181 gccagcgctg gatcttggtc tttgcctcgt cggcagcctg ggagaactgt ttttgcgcct
     6241 tccagcccgc cttgatcgcg ttcttccaat cctcgtccac ggtaccctcc tgccatcaag
     6301 ccgtatcata tcggccccgg cttcgacaac cgctttccgg acggtcttcc gcgcaccgca
     6361 ctgccaggca gccgcgatgc ccctccttcc tgagctccgg cgcttggcga gggtcagtac
     6421 gtgaccgcgg cggtcgcgac gccgccgtag tagcggtcgg tcgcgccgtg gacgtcgacg
     6481 gtcgacggat caccgtcgcg gctgttcgcc agcatgccgg tgccggtgaa cgacagattc
     6541 agctgggtcg cctcggccgc gaggcggatc agcagccgct cggtgatgag cagctcgacg
     6601 ccggccgacg cggtgccgcc gagcacgcgg gccgcgccgt actggtcggc gcgctggatc
     6661 gggccggccg acgtgacgat caggccctgt ccggcgagcg cgaccgcgac gtggccgccg
     6721 agcggcaggc ggaacgccac gccggggtcg aacatccggt agtcgacgtc gggcaggtcg
     6781 agcgagctcg gcgactgcag gccgcggcga tcgaccgcga accggcgcgc gccgtagccg
     6841 gcgcccagcg tcagggtcgg gctggtcggc tggtggccga acgcgagccg gtagcggaca
     6901 ctgaacgcgt agtgccgctc ggtggccgcg agcggcaccg tcatctcgtt cgaggcgcgc
     6961 agcgtgagcg acggcgtccg atcgtagtcg gccgcgacgc cgaacccggc gagccagctc
     7021 gccggtgcga tcagcgcgac cgggtagagc tcgccggcga cccgcgcgcc gggcacgggg
     7081 gcgttgcggt acgaccgcgg cgagccgtcg aacgtccggg tgtcgaacgt gaggcggcgg
     7141 ccggccatcg ag
//
LOCUS       DASVOR010002238         4530 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_403269,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002238 DASVOR010000000
VERSION     DASVOR010002238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4530
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403269"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1723
                     /locus_tag="VF516_41395"
     CDS             <1..1723
                     /locus_tag="VF516_41395"
                     /inference="COORDINATES: protein motif:HMM:NF021841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070042 - rRNA
                     (uridine-N3-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rossmann-like fold-containing protein"
                     /protein_id="HEX8114278.1"
                     /translation="APRPGELEPQPRSTGGERDQTATPAHDETHAPAQRLASNDAVVE
                     QLAHGMVDRDLTPADEAFLQANGYRTEPPIRGEHQFVMRVFYPTEEGKPPIVAFRGTV
                     PSKPATIIADLDPRGIGTWQFNANRSAIEHAVNKASEYGPVVFAGHSLGGALAQTAAS
                     HFPAQADRIVTFQAPGVSRGTVRAVEDFNAAHPDNPILSTHHRVTGDLVPRGGRALTP
                     GTVYDHEMFGGSRLSRNPLSKHTSLPLAQEEIAAGHQVPISRDGQQFRSTGDHSTAED
                     NAGKNQVIENVRTGLGGVIYGPGLAVSSVRRLFGPPRERTSLPTGEPVHELPPGETLP
                     HEPTGSNEPLPPPAPRPGAEIGLPPPAPRPGEHEPTPEPEAGIPGQQEPHRVLAVGES
                     TFEYSQALRNQLDAEGAGQNTDIIASGLQGRQEALGMRANQNVTEPLVDGPGYHIQHQ
                     VDATRLEEKFEPNSLDRIIWNNPEAPDTGAVINDLLKSAPKVLRPGGQINIGTTASPR
                     AKGADVLAQHVEAGTITVEGHRYQVTVVPNTEYPYAVSYPSRRTTGGRLRRAQGPKLY
                     YIFKLLD"
     gene            1737..2369
                     /locus_tag="VF516_41400"
     CDS             1737..2369
                     /locus_tag="VF516_41400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114279.1"
                     /translation="MPDDTNEHFFACNLLWWSLRVSFGRSELLQVDELAERIEEYDDR
                     LEELGAWLMAEFVRAGPTYLVDGQKKGISSIDPAEIEQRLTRSLKALEELAVSVAPTA
                     ESRAYCAYVAECSWVVAILRAFADLERNLSSDDASARYREIQDECTDLIGWIEVQRDA
                     VAAERIPADSRGALHGKIEPVIDAAVRVWRDEVRAYSSRYPRSGSEARRS"
     gene            2366..2935
                     /locus_tag="VF516_41405"
     CDS             2366..2935
                     /locus_tag="VF516_41405"
                     /inference="COORDINATES: protein motif:HMM:NF024922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEX8114280.1"
                     /translation="MIVPLANTEASIPGLRLASHVLSEHEESELLRLIEHWSDGWERP
                     VLRRGLLPARREMRCFGWSYVTRGRTLTECEALPHELGAAMKRWLENGGVEAALLEQV
                     IVTRYRPGAGIGWHTDAPVFGPVVATLSLGADWRMEFRRRAGDKAIRIALPQRSLLLL
                     AGEARTHWQHRIPPVKETRVSLSFRTVRS"
     gene            complement(2943..3695)
                     /locus_tag="VF516_41410"
     CDS             complement(2943..3695)
                     /locus_tag="VF516_41410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114281.1"
                     /translation="MPDLAALMQAVVDAPTEDAPRAAYARAVAATDPERAELIDIQLE
                     LARARRAHTEDGHPALNREGTLIRTHGARWAADVRPLVDSWQFLRGFTDVVTLDAARF
                     LTTAPELYRRAPVLHLNLTGVQPVAAELFQSPHLARIQSLNLRRNALGDAEVAALAAS
                     PHLANLRWLELGINKIGAAGLEALAASRNLPRLGYVGLATNAIPDPTPRHADEYDATS
                     PVGEELQRKYGPRPWLDARPRWQWPPPRDAVD"
     gene            3760..4197
                     /locus_tag="VF516_41415"
     CDS             3760..4197
                     /locus_tag="VF516_41415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114282.1"
                     /translation="MTTPSRVVWRMGNPSAPNSPWGRDELDVLGDGRVQYRNQRGAVA
                     RSVTAQLAPGAAEKLFAAIAASPFPVWTDTRPLPPGSTLVELTVERPDGAATARFDYH
                     TSLKTPGYDAVVRLLTGWAGVLRSPPDKRAASAELTQIVDQPG"
     gene            4197..>4530
                     /locus_tag="VF516_41420"
     CDS             4197..>4530
                     /locus_tag="VF516_41420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114283.1"
                     /translation="MVEVPGRPRSLAEMQFFAGLRPCAACGDHQPVAWRVGSWCARPE
                     GGLRTGADRGQGARWTLRGACTRCATQRSFEFVCEGDPIEAVHGDLELGGAEPSTVLD
                     PYLLVGEID"
BASE COUNT          696 a         1585 c         1622 g          627 t
ORIGIN      
        1 ggcgccccgg cccggcgagc tcgagcccca gccgcgctcg accggcggcg agcgcgacca
       61 gaccgccacc ccggcgcacg acgagaccca cgcgccggcc cagcggctcg ccagcaacga
      121 cgccgtcgtc gagcagctcg cccacggcat ggtcgaccgc gacctcacgc cggcggacga
      181 ggcgttcctc caggccaacg gctaccgcac cgagccgccg atccgcggcg agcaccagtt
      241 cgtgatgcgg gtgttctacc cgaccgagga gggcaagccg ccgatcgtcg cgttccgcgg
      301 caccgtgccg tcgaagccgg cgaccatcat cgccgacctc gatccgcgcg gcatcggcac
      361 gtggcagttc aacgccaacc gctcggcgat cgagcacgcg gtcaacaagg cctcggagta
      421 cggcccggtg gtgttcgccg ggcacagcct cggcggcgcg ctggcccaga ccgcggcgtc
      481 gcacttcccg gcccaggccg atcgcatcgt caccttccag gccccgggcg tgtcgcgcgg
      541 cacggtgcgg gccgtcgagg acttcaacgc cgcgcacccc gacaacccga tcctctcgac
      601 ccaccaccgc gtgaccggcg acctcgtgcc gcggggcggc cgggcgctca cccccggcac
      661 cgtctacgac cacgagatgt tcggcggcag ccggctcagc cgcaacccgc tgtccaagca
      721 cacctcgctg ccgctcgccc aggaggagat cgccgccggg caccaggtcc cgatctctcg
      781 cgatggccag cagttccgct ccaccggcga ccactcgacc gccgaggaca acgcgggcaa
      841 gaaccaggtc atcgagaacg tgcggaccgg cctcggcggc gtgatctacg gccccggcct
      901 cgccgtgtcg tcggtccggc ggctgttcgg cccgccccgc gagcgcacct cgctgcccac
      961 cggcgagccg gtacacgagc tgccgccggg cgagaccctg ccgcacgagc caaccggctc
     1021 gaacgaaccg ctgccgccgc ccgccccgcg ccccggcgct gagatcggcc tgccgccgcc
     1081 agcgccgcgg cccggcgagc acgagcccac gcctgagccg gaagctggta ttcctggcca
     1141 gcaggagccg caccgcgtgc tcgccgtcgg cgaatccacg ttcgagtaca gccaggctct
     1201 ccgcaaccag ctcgatgccg aaggggcggg gcagaacacg gacatcatcg cgtcagggct
     1261 gcaagggcgc caggaagcgc ttggcatgcg cgcgaatcag aacgtcacgg agccactggt
     1321 cgacggcccc gggtaccata tccagcacca ggtcgatgcg acgcggctgg aagagaagtt
     1381 cgagccgaac tcgctcgacc ggatcatctg gaacaacccc gaggcgcctg acaccggcgc
     1441 cgtcatcaac gacctgctca agagcgcccc gaaagtgctg cggcctggcg gccagatcaa
     1501 catcggaacc actgcttctc ccagggccaa aggcgcagat gtcctggcac agcacgtcga
     1561 agcgggaacc atcacggtcg aaggtcaccg ctaccaggtg acggtcgtgc cgaacacaga
     1621 atatccctac gcggtatcgt acccttcgcg acgaacaacg ggcggcaggc tccgaagagc
     1681 gcagggcccc aagctgtact acatcttcaa gctgctggac tgagtcgtac agcccgatgc
     1741 ccgacgatac gaacgagcac ttctttgcgt gcaacctgct ctggtggagc ctgcgcgtca
     1801 gcttcggtcg ttccgagctc ctgcaggttg acgagctggc ggaacggatc gaggagtacg
     1861 acgatcgtct cgaagagctg ggggcgtggc tcatggctga gttcgtgcga gccggcccca
     1921 cctacctggt cgatgggcag aagaaaggga tctcgagcat cgatcccgca gagatcgagc
     1981 agcgactcac gcgctcgctg aaggcgctcg aggagcttgc cgtctctgtc gctcccaccg
     2041 ccgagtcccg agcgtactgc gcctatgtcg ccgaatgtag ctgggtcgtg gcgatcctgc
     2101 gtgcgttcgc cgacctggag cgcaatctgt ccagcgacga cgcatcggcc aggtaccgtg
     2161 agatccaaga cgaatgcacg gatctgatcg ggtggatcga ggtccagcga gacgcggtcg
     2221 cagcggagcg gatcccggca gactcgcgcg gtgcgttgca tggcaagatc gagcctgtca
     2281 tcgacgccgc tgtgcgggtg tggcgcgacg aggtgcgcgc ctatagttcg aggtacccgc
     2341 gctcggggtc cgaggcacgg cgttcttgat cgttcccctc gcgaacacgg aagcgagcat
     2401 cccggggctg cgactcgcgt cccacgtgct ctcggaacac gaagaatccg agctgcttcg
     2461 cctgatcgag cactggtccg atgggtggga acgaccggtg ctacgccggg gcttgctgcc
     2521 agcgcgccgc gagatgaggt gcttcggatg gagctacgtc acacgagggc gcacgctgac
     2581 cgagtgtgaa gcattgccgc acgagctcgg cgctgcgatg aaacgatggc tcgagaacgg
     2641 cggcgtcgaa gctgcgctgc tcgagcaggt gatcgtgacc cgctaccgtc ccggggcagg
     2701 gatcggctgg cacacggacg cgccggtgtt cgggcccgtg gtcgcgaccc tgtcgctcgg
     2761 agctgactgg cgcatggagt tccgtcgccg tgccggagac aaggccatcc ggatcgcgct
     2821 accgcagcga tcgctgctcc tcctcgcggg agaggcgcgc acccactggc aacaccgcat
     2881 ccccccggtg aaggaaaccc gtgtgtcgct ctcgttccgc accgtgcggt cgtgagtcca
     2941 tattagtcga ccgcgtcgcg cgggggcggc cattgccacc gggggcgcgc gtcgagccag
     3001 ggccggggac cgtacttgcg ctggagctcc tcgccgaccg gtgaggtcgc gtcgtactcg
     3061 tcggcgtgcc gcggcgtcgg atccgggatg gcgttggtcg cgaggccgac gtagcccagg
     3121 cggggcaggt tgcgcgacgc ggcgagcgcc tcgaggccgg cggcgccgat cttgttgatg
     3181 ccgagctcga gccaccggag gttcgccagg tggggcgagg ccgcgagcgc ggcgacctcc
     3241 gcgtcgccga gcgcgttgcg gcgcaggttc agcgactgga tgcgcgcgag gtggggggac
     3301 tggaacagct cggcggccac cggctggacg ccggtgaggt tgaggtggag caccggcgcg
     3361 cggcggtaca gctcgggggc ggtggtgagg aaccgcgcgg catcgagcgt gaccacatcg
     3421 gtgaacccgc gcaggaactg ccagctgtcg accagcggcc ggacgtccgc ggcccaccgt
     3481 gcgccgtgtg tgcggatcag cgttccctcg cggttgaggg cagggtgacc gtcctccgtg
     3541 tgtgcccggc gcgcgcgggc cagctcgagc tgaatatcga tcagctcggc gcgctccggg
     3601 tcggtcgcgg cgaccgcgcg ggcgtaggcg gcgcgcggcg cgtcctcggt cggcgcgtcg
     3661 acgacggctt gcatcagcgc ggcgagatcg ggcacgcgga ggagcgtagc agagccggcg
     3721 cggctggtgg ccctccgggc gatctcgtat cctcgtgcca tgacgacccc gagccgcgtc
     3781 gtatggcgga tgggcaatcc cagcgccccg aactcgccgt ggggccgcga cgagctcgac
     3841 gtgctcggcg acggccgggt gcagtacaga aaccagcgcg gcgcggtggc gcgatcggtc
     3901 accgcgcagc tggcgccggg cgccgcggag aagctgtttg ccgcgatcgc ggcgtcgccg
     3961 ttcccggtgt ggaccgacac ccggccgctg ccgccgggca gcacgctggt cgagctcacc
     4021 gtcgagcggc cggacggtgc ggcgaccgcg cggttcgact atcacacgtc gctcaagacc
     4081 ccgggctacg acgcggtggt ccgcctgctc accggctggg ccggcgtgct gcgctcgccg
     4141 ccggacaagc gcgcggcgtc ggccgagctg acccagatcg tggaccagcc ggggtagtgg
     4201 tggaggtccc gggccgtccg cgctcgctcg ccgagatgca gttcttcgcc gggctgcgtc
     4261 cgtgcgccgc gtgcggcgat caccagccgg tcgcgtggcg cgtgggttct tggtgtgctc
     4321 gtcctgaagg gggattgcgg acgggcgcag accgcgggca gggcgcgcgc tggacgctgc
     4381 gcggcgcgtg cacgcggtgc gcgacccagc gcagcttcga gttcgtgtgc gagggcgatc
     4441 cgatcgaggc ggtccacggc gatctcgagc tcggcggcgc cgagccgtcg acggtgctcg
     4501 acccgtacct gctggtcggc gagatcgatc
//
LOCUS       DASVOR010002239         1596 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_520619,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002239 DASVOR010000000
VERSION     DASVOR010002239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520619"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF516_41425"
     CDS             complement(<1..180)
                     /locus_tag="VF516_41425"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HEX8114284.1"
                     /translation="MTALGAQILADLYECDPAALADEDVVRDAMLEAARRCGATIVAQ
                     CFHRFSPHGISGVVVI"
     gene            complement(177..>1596)
                     /locus_tag="VF516_41430"
     CDS             complement(177..>1596)
                     /locus_tag="VF516_41430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8114285.1"
                     /translation="SMCWPDIDSFAREVALPPGVRVRQLARADIPTLIRLLAEWHPGF
                     AVMEDASLLEERYYTENVALTGERHTTMERPVYVTLLESEGAPVGCLVCEVEADGAVH
                     AGRLSVVAPHFRRRRLGRANIDLHIALARATGASVIYALVDLDNLAQHALLEGAGLSL
                     GGILPGSEWKQVGSERIQGVPEAVRFKTLVSADELIWPEPAALRPATAGLMKLLFDYG
                     DPPGPLVSFRPRPVPDLDDAARALLEARRGGHLEWPDARLLAGRLALPPVVTVRRLAR
                     ADLPGLIDRLPAWHPDIAHGSQRRLLSPAFYEARVALEGEDAHIERRPAHVWVVERGG
                     ELAGLSVVRCRLEHDALFAEFIVIDPRHRGAGLARILLHTAVLAARAVGVEIISAFTT
                     LCHPYGQRLCEGAGFRLIGILPAANRHTVSPGVVKHAFEAVYSISLLSPEQSYTPSRE
                     AMPPRVAALADFVYGLPEEALA"
BASE COUNT          276 a          546 c          573 g          201 t
ORIGIN      
        1 gatcacgacc acgccgctga tgccgtgggg actgaagcgg tggaagcact gggccacaat
       61 cgtggcgccg cagcgccgcg cggcctcgag catggcgtca cggaccacgt cctcgtcggc
      121 aagcgccgcg ggatcgcact cgtacaggtc tgcgaggatc tgtgcgccga gcgcggtcat
      181 gccagcgcct cctcggggag cccgtagacg aagtcagcga gggcggccac gcgcggcggc
      241 atcgcctcgc gcgagggcgt gtagctctgc tcgggcgaca gcagcgagat cgaatagacc
      301 gcctcgaagg cgtgcttcac gacccccggg gagaccgtgt gtcgattcgc cgcgggcaag
      361 atgccgatga gccggaagcc cgcgccctcg cagagacgct ggccataagg gtgacatagc
      421 gtggtaaagg cgctgattat ctccacccca acagcgcgcg cagcgagcac ggcggtgtgt
      481 aacaggatgc gcgcaagacc cgcaccgcgg tgccgcggat cgatgacgat gaactccgcg
      541 aacaacgcgt catgctccag acggcagcgc acgaccgaca gtccggcgag ctcgccgccg
      601 cgctccacga cccagacgtg cgccgggcga cgctcgatgt gcgcgtcctc gccttcgagc
      661 gcgacgcggg cctcgtagaa ggcaggcgag agcaggcggc gctgcgagcc gtgcgcgatg
      721 tccggatgcc acgcgggaag tcggtcgatg agccccggga ggtcggctcg agcgagtctc
      781 cgcacggtca cgacgggggg gagggcgagc cgcccagcga ggagacgggc atcgggccac
      841 tcgaggtgtc cgccgcggcg agcctcgagg agcgcccggg ctgcgtcatc gagatcaggc
      901 acgggccgcg gccggaacga cacgagaggg ccaggcggat cgccgtaatc gaacaggagc
      961 ttcatcaggc cagcggtcgc ggggcgcagc gccgctggct ccggccagat gagctcatcg
     1021 gcggagacga gcgtcttgaa acgaacggcc tccggaacac cctgtatccg ctccgagccg
     1081 acctgcttcc actccgagcc cggcaggatg ccacccaggg acagcccggc tccctccagc
     1141 agggcgtgct gggccaggtt gtcgagatcg acgagagcat atatcacgct cgcgccggtc
     1201 gctcgcgcca gcgcgatatg cagatcgatg ttcgcgcgcc cgagcctgcg tctgcgaaag
     1261 tgcggggcca caacggagag gcggccggcg tggaccgccc cgtccgcctc aacctcgcac
     1321 acgaggcagc ccacgggcgc gccctcgctc tccagcaggg tcacgtacac ggggcgttcc
     1381 atggtcgtgt gcctctctcc ggtcagggcc acgttctccg tgtaatagcg ctcctcgagc
     1441 agcgaggcgt cctccatgac ggcgaacccg gggtgccact ccgcgagcag tcggatcagg
     1501 gtggggatgt ccgcgcgggc gagctgccgc acccgcacgc cgggcggcag cgcgacctcg
     1561 cgagcgaagg agtcgatgtc aggccagcac atgctc
//
LOCUS       DASVOR010002240         1703 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_199766,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002240 DASVOR010000000
VERSION     DASVOR010002240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199766"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            670..1611
                     /locus_tag="VF516_41435"
     CDS             670..1611
                     /locus_tag="VF516_41435"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8114286.1"
                     /translation="MEVSNRTAAIHLGLFAACCATTWWAGGPAFAATLMTILLCHELG
                     HYVVSRRHRIDVSLPYFIPLPPIISVGTLGAVIRMRKSIEDRRALFDVGAAGPVAGLV
                     IAIPLLVVGLMLSQLEPIQRGDMLEGNSILYALLKYAVFGRWLPSGGVDVRLHPMANA
                     GWFGLFLTVINLMPIGQLDGGHIARAALGQSHETWSARLHVALPIVGLAIGASMFRVA
                     RNAGVDLGEALSYASSGLLPYLVWTLLLGWMRRQAGEYHPPVHDAPLDPRRRRYAFAM
                     LVLFFLIATPVPFRPALCQDLAEPQQCASSANPAVSS"
BASE COUNT          212 a          582 c          617 g          292 t
ORIGIN      
        1 ggccggccgc accgtggcgg tcgtgctctc cggcgcgaat atcgacgagc cgacgctgcg
       61 gcgcgtgctg aaccgcgaga tctgatcgcg gtcacggcgc gaccgccgcc ggagatgcag
      121 aagaccccgg ggacaggccc acgcgccgcg cgcacacgca cggtggcgcg tccggcggca
      181 cggtgcgcgg tgactcgaca cccgcgcggc ggccgatcat gaggccgtcg atcatgaggt
      241 gagcatcacc tggccattcg taatgaacat gaagaatcac cagagctctc gcttctccgc
      301 gcacgggcag gcagtcgcat ggcgagctgt cgtcgggccc ggcgatccgt cgcggggcgc
      361 acgagccgcc gtgctgcagt cgggtggcaa tctagcggcg gatacatagt gtgtcaatga
      421 catgttcgtt agttcctaat atgcgcttga gtgtcgcgtt gcggtgatcg atcggtcggt
      481 cggtcggtcg gctggctgtc tctccgcgcc ggccgcgccg gctgcgccgc gcacgagcgg
      541 tcgcgcggag atccggtgcc gcggtggcat cacctcacgg cgggttcgcg ccgtgctcgc
      601 tgtgcctggg cgcactgtcg ggcacgctcg atcccggcgc gccgcggggt ttgcggctac
      661 actcggacgg tggaagtcag caaccgcacc gccgcgatcc atctcggcct gttcgcggcg
      721 tgctgcgcga ccacctggtg ggccggcggc ccggcgttcg cggccacgct gatgacgatc
      781 ctcttgtgcc acgagctcgg ccactacgtg gtgagccggc gccaccggat cgacgtctcg
      841 ctgccgtact tcatcccgct gccgccgatc atctcggtgg gcacgctcgg cgcggtgatc
      901 cgcatgcgca agtcgatcga ggatcgccgc gcgctgttcg acgtcggcgc ggcgggcccg
      961 gtcgccgggc tggtcatcgc gatcccgctg ctggtggtcg gcctgatgct gtcgcagctc
     1021 gagccgatcc agcgcgggga catgctggag ggcaactcga tcctctatgc gctgctcaag
     1081 tacgcggtgt tcggtcgctg gctgccgtcg ggcggggtcg acgtccggct ccatccgatg
     1141 gcgaacgccg gctggttcgg cctgttcctc acggtgatca acctgatgcc gatcggtcag
     1201 ctcgacggcg gccacatcgc gcgcgcggcg ctcggccagt cgcatgagac ctggtcggcg
     1261 cggctgcacg tcgcgctgcc gatcgtgggg ctcgcgatcg gcgcctcgat gttccgggtg
     1321 gcgcggaacg ccggcgtcga cctcggtgaa gcgctgagct acgcgtcgtc gggcctcttg
     1381 ccgtacctgg tgtggacgct gctgctgggc tggatgcggc ggcaggccgg cgagtaccac
     1441 ccgccggtcc acgacgcgcc gctcgatccg cggcggcggc gctacgcgtt cgcgatgctc
     1501 gtcctgttct ttctgatcgc gacgccggtg ccgttccggc ccgcgctgtg ccaggatctc
     1561 gcagagcctc agcaatgcgc gtcgagtgcg aatcctgccg tgagctcgtg actgcgagct
     1621 tcgcgctcgc cgggcgaatg gataccggaa cgctgcgcgc gacctgcccg gcgtgcggcc
     1681 acgcgatgac cgtgccgatg gcg
//
LOCUS       DASVOR010002241         2973 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_170208,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002241 DASVOR010000000
VERSION     DASVOR010002241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2973
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170208"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            396..866
                     /locus_tag="VF516_41440"
     CDS             396..866
                     /locus_tag="VF516_41440"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEX8114287.1"
                     /translation="MDATDLRILEILQRDARATQQEIARRVRLSQPSVADRIHKLEAR
                     GVILGYIAKLDPAQLGKDITAFIGVGISHPKYFEQFGKKILAMPDVLECHRVAGSDSY
                     LLKIRTENTRSLDRLLVEHIRAIPGVYRTETTLVLSSIKEDVALSIHAEQEASP"
     gene            863..2134
                     /locus_tag="VF516_41445"
     CDS             863..2134
                     /locus_tag="VF516_41445"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEX8114288.1"
                     /translation="MNSDRSFSGLIPIAIPPLEPPVPSAAPAHSAPRLPRLACRMADM
                     PPSAVREILKVAERPDILSFAGGLPAPELFPVAAIADAHADVLRDDGGKALQYSTTEG
                     FGPLRAWIATHLRTRGIPARPEELLITNGSQQGIDLVSRVFLDPGDVVLVDNPTYLAA
                     IQSFAAYQVRLVTVPSDDDGMRVDDLADLIAAHRPKLIYVVSSFQNPRGTTLPQERRA
                     EIAELAAQSGVAIIEDDPYGELSFDGPPPRPIAAAGSGNVLYLGTFSKTLAPGLRIGW
                     LWGDAGIVRKATICKQAADLHTATLAQRATASLLGRFDYAGHVARICEVYRERCQTML
                     GALAEYMPDGSRWVAPTGGMFVWLQLPAQLDAEALFPEAIARKVAFVPGAAFFVEGHR
                     RDFVRLNFSNQPPLSIVEGMRRLGEVVAAAL"
     gene            2239..>2973
                     /locus_tag="VF516_41450"
     CDS             2239..>2973
                     /locus_tag="VF516_41450"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8114289.1"
                     /translation="MFTSWRLGRVLGFPVEINLSFVLLLVAVLFGFGGFGFGFGGLVM
                     VGLVFGSVLLHELGHALVARRYGVHVSGIELSFFGGAAKMVEMPRSANHEIAIAAAGP
                     AVSLMLTGVGFGLGALAPFSPLGEWLLKIGGINLMLAAFNLIPALPMDGGRILRAALT
                     RRMDFVRATDIAVDIARVVAIGFGVIGLVSPGWIMLLALAPLLWMMGTQERMLARAMV
                     DRYAGFGRPSVDPFDRWGRAASRFDRP"
BASE COUNT          398 a         1087 c         1009 g          479 t
ORIGIN      
        1 gcgcgcgcgc gagctggcgg acgcgctcgg catcccgggc tacccgtcgt ccggagcctg
       61 accgctccgc gctcgcgccg ctcgtccccg caccggccga ccgaccgggc ggttgcagcg
      121 tcgatcgacg aaccgggggt ctgacaccac agcggaacag aacaccgtcg ctgggggcga
      181 aacaggcgcg aaacacggag gacgcacacc gtgcacacgc agcgctcccg gcgcgatctc
      241 acgcaccgca tcgcccggga tcggcagtct gtgccgtcgc gcacggacgc gacgcgttac
      301 cgcgatcgga tcccgctcct atacaaaata ggtttgccac ccgcaccgat cgatttctga
      361 ttgatgcgcc tcccgcgctc tcctatggtt agtacgtgga cgccaccgac ctgcggatcc
      421 tggagatcct tcagcgcgac gcgcgtgcga cgcagcagga gatcgcgcgc cgggtccggc
      481 tatcgcagcc cagcgtcgcc gatcggatcc acaagctcga ggcgcgcggc gtgatcctgg
      541 ggtacatcgc caagctcgat cccgcgcagc tcggcaagga catcaccgcg ttcatcggcg
      601 tcgggatctc gcacccgaag tacttcgagc agttcggcaa gaagatcctc gccatgccgg
      661 acgtgctgga gtgccaccgc gtcgcgggct cggactcgta cctgctcaag atccggaccg
      721 agaacacccg ctcgctcgat cgcctgctgg tcgagcacat ccgagcgatc cccggcgtgt
      781 accgcaccga gaccacgctg gtgctgtcct cgatcaagga ggacgttgcg ctgtcgatcc
      841 acgccgaaca ggaggcctcg ccatgaactc cgaccgttcc ttctccggcc tgatcccgat
      901 cgcgatcccg ccgctggagc ctcctgtgcc gagcgccgcg cctgcgcact ccgcgccgcg
      961 gctgccgcgg ctggcgtgcc ggatggcgga catgccgccg tcggcggtgc gcgagatcct
     1021 caaggtcgcc gagcgccccg acatcctgtc gttcgccggc gggctgccgg cgccggagct
     1081 gttcccggtc gcggcgatcg ccgacgctca cgccgacgtg ctgcgcgacg acggcggcaa
     1141 ggcgctgcag tactcgacca ccgagggctt cggcccgctg cgcgcgtgga tcgccaccca
     1201 cctgcgcacg cgcggcatcc cggcgcgccc cgaggagctg ctgatcacca acggctcgca
     1261 gcagggcatc gatctggtgt cgcgcgtgtt cctcgacccc ggcgacgtcg tgctggtcga
     1321 caacccgacc tacctggcgg cgatccagtc gttcgcggcc taccaggtcc ggctggtcac
     1381 cgtgccgagc gacgatgacg gcatgcgggt cgacgacctc gcggacctga tcgccgcgca
     1441 ccgacccaag ctgatctacg tggtgtcgag cttccagaac ccgcgcggca ccacgctgcc
     1501 ccaggagcgg cgcgccgaga tcgccgagct cgcggcgcag agcggcgtcg cgatcatcga
     1561 ggacgatccg tacggcgagc tgtcgttcga cgggccgccg ccgcgcccga tcgccgcggc
     1621 cggctccggc aacgtgctct acctcggcac gttctccaag accctggcgc ccggcctgcg
     1681 gatcggctgg ctgtggggcg acgccggcat cgtgcgcaag gcgacgatct gcaagcaggc
     1741 ggccgacctg cacaccgcga cgctcgccca gcgcgcgacc gcgtcgctgc tcggccgctt
     1801 cgactacgcc ggccacgtcg cgcggatctg cgaggtctac cgcgagcgct gccagaccat
     1861 gctcggcgcg ctcgccgagt acatgcccga cggcagccgc tgggtcgcgc cgaccggcgg
     1921 gatgttcgtg tggctgcagc tgccggcgca gctcgacgcc gaggcgctgt tccccgaggc
     1981 gatcgcccgc aaggtcgcgt tcgtgccggg cgcggcgttc ttcgtcgagg gccaccgccg
     2041 cgacttcgtc cggctgaact tctcgaacca gccgccgctg tcgatcgtcg agggcatgcg
     2101 gcggctcggc gaggtcgtcg ccgccgcgct gtgatccgag cggatcggtc gcaggacctg
     2161 ccatttcagg caggtttcac ccgttgcgga aatatgtgcg cggtttgaca accggacgcc
     2221 ggtgcccatg ttgtagccat gttcacgtca tggcgactgg gccgggtgct cggcttcccg
     2281 gtcgagatca acctcagctt cgtcctcttg ctcgtggcgg tgctgttcgg gttcggcggg
     2341 ttcggctttg gcttcggcgg cctggtcatg gtcggcctgg tgttcggctc ggtgctgctc
     2401 cacgagctcg gccacgcgct ggtcgcccgc cgctacggcg tccacgtgtc cgggatcgag
     2461 ctgtcgttct tcggcggcgc ggccaagatg gtggagatgc cgcgctcggc gaatcacgag
     2521 atcgcgatcg ccgcggcagg tccggcggtg tcgctgatgc tcaccggcgt cgggttcggc
     2581 ctcggcgccc tggcgccgtt ctctccgctc ggcgagtggc tgctcaagat cggcgggatc
     2641 aacctgatgc tggccgcctt caacctgatc ccggcgctgc cgatggacgg cggtcggatc
     2701 ctgcgcgccg cgctcacccg ccggatggac ttcgtgcgcg cgaccgacat cgccgtcgat
     2761 atcgcccgcg tggtcgcgat cgggttcggc gtcatcggtc ttgtttcgcc cggctggatc
     2821 atgctgctcg cgctcgcgcc gctgctctgg atgatgggca cccaggagcg catgctggcg
     2881 cgcgcgatgg tcgaccgcta cgcgggcttc ggccgcccca gcgtcgaccc gttcgaccgc
     2941 tggggccgcg ccgccagccg gttcgaccgc ccg
//
LOCUS       DASVOR010002242         1796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_492099,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002242 DASVOR010000000
VERSION     DASVOR010002242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492099"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1606)
                     /gene="carB"
                     /locus_tag="VF516_41455"
     CDS             complement(<1..1606)
                     /gene="carB"
                     /locus_tag="VF516_41455"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX8114290.1"
                     /translation="MPRRTDLESVLIIGSGPIVIGQACEFDYSGTQACKALREEGLRV
                     VLINSNPATIMTDPELADATYVEPLTVDVLDKVLGVEKVTAILPTLGGQTGLNLAMAA
                     SKAGLLDKHRVELLGAQVATIEMAEDRELFKQAMARIGLSCPRSRLVGSVAEARACVD
                     EIGFPAILRPSFTLGGSGGGIAYNRAELDRMVQFGLDQSPVHSILIEESVLGWKEYEL
                     EVVRDAKDNAVIVCSIENLDPMGVHTGDSITVAPAMTLTDKEYQRMRDAAIAILREIG
                     VTTGGSNVQFAVDPKTGRMLVIEMNPRVSRSSALASKATGFPIAKIAAKLAIGYTLDE
                     LHNDITRVTPASFEPTIDYVVVKWPRFAFEKFPSATPVLGPQMKSVGEAMAIGRTFRE
                     ALGKAIRSLETGRAGFDLPMTALGDDLAQIERAIATPSPDRLYQIGHGFQLGLTVERA
                     HELTRIDPWFLHHIRAIATAEQALAGAPLARLTAADLRRAKRIGMSDRRIAALTGARE
                     AEVRAARHAHGVRPVYKRVDTCGAELE"
BASE COUNT          243 a          655 c          623 g          275 t
ORIGIN      
        1 actcgagctc ggcgccgcag gtgtcgaccc gcttgtagac cggccgcacg ccgtgggcgt
       61 ggcgcgcggc gcggacctcg gcctcccggg cgccggtcag cgccgcgatc cggcgatcgc
      121 tcatcccgat ccgcttggcg cgccgcaggt cggccgcggt cagccgggcg agtggcgcgc
      181 cggccagcgc ctgctcggcg gtggcgatcg cgcggatgtg gtggaggaac cacggatcga
      241 tccgggtcag ctcgtgggcg cgctcgacgg tcagcccgag ctggaagccg tgcccgatct
      301 ggtacagccg gtcgggcgac ggcgtcgcga tcgcgcgctc gatctgggcg aggtcgtcgc
      361 cgagcgcggt catcggcagg tcgaacccgg cccgcccggt ctcgagcgac cggatcgcct
      421 tgccgagcgc ctcgcggaac gtccggccga tcgccatcgc ctcgccgacc gacttcatct
      481 gcggaccgag cacgggcgtc gccgacggga acttctcgaa cgcgaaccgc ggccacttga
      541 cgaccacgta gtcgatcgtc ggctcgaacg acgccggggt gacccgggtg atgtcgttgt
      601 ggagctcgtc gagcgtgtag ccgatcgcga gcttggcggc gatcttggcg atcgggaacc
      661 cggtcgcctt cgacgccagc gcgctcgacc ggctgacccg cggattcatc tcgatcacca
      721 gcatccggcc ggtcttcgga tcgaccgcga actgcacgtt gctgccgccg gtggtcacgc
      781 cgatctcgcg caggatcgcg atcgcggcgt cgcgcatccg ctggtactcc ttgtcggtca
      841 gcgtcatcgc cggcgcgacc gtgatcgagt ccccggtgtg gacccccatc gggtcgaggt
      901 tctcgatcga gcacacgatc accgcgttgt ccttggcgtc gcgcacgacc tcgagctcgt
      961 actccttcca gcccagcacg ctctcctcga tcaggatcga gtggaccggc gactggtcga
     1021 ggccgaactg gaccatgcgg tcgagctcgg cgcggttgta cgcgatgccg ccgcccgagc
     1081 cgccgagcgt gaacgacggc cggaggatcg ccgggaagcc gatctcgtcg acgcacgccc
     1141 gcgcctcggc gacgctgccg accagccgcg accgcgggca ggaaaggccg atccgcgcca
     1201 tcgcctgctt gaacagctcg cggtcctcgg ccatctcgat cgtcgcgacc tgtgcgccga
     1261 gcagctcgac cctgtgcttg tcgagcagcc cggccttgct cgccgccatc gcgaggttga
     1321 gcccggtctg gccgcccagc gtcggcagga tcgccgtcac cttctcgacg ccgagcacct
     1381 tgtcgagcac gtcgacggtc agcggctcga cgtacgtggc gtcggcgagc tcgggatcgg
     1441 tcatgatcgt cgccgggttc gagttgatca ggacgacgcg caggccctcc tcgcgcagcg
     1501 ccttgcacgc ctgggtcccc gagtaatcga actcgcaggc ctgaccgatc acgatcggcc
     1561 ccgagccgat gatcagcacc gattcgaggt ccgtacgtct cggcatcggc cggacgtgta
     1621 ccacaccggc ggcgacggat cagcccggga acccccggcg cgggacgatc gtcttcccgg
     1681 ccgtgccgcc gggatcagcg cttcttgacc gcgccgccct cgaccttgaa catgtcggcg
     1741 agcgggccgg tcagcgccgc ggtcaccgcg gccgcgtggt cgccgagcgg cgcccg
//
LOCUS       DASVOR010002243         1802 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_25662,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002243 DASVOR010000000
VERSION     DASVOR010002243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25662"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..401
                     /locus_tag="VF516_41460"
     CDS             <1..401
                     /locus_tag="VF516_41460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114291.1"
                     /translation="YVSATHQRGRDRGPTVWTWYYPFPGDARTARTQLAGATYEDWAE
                     VVLADLERAHPDVRGLVDHIEVAFWGHGMIRPRTGALFGGGGMAGRIERAAPVGRLHF
                     AHTDLSGLALFEEAFDHGLRAAREVAEALT"
     gene            398..1477
                     /locus_tag="VF516_41465"
     CDS             398..1477
                     /locus_tag="VF516_41465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114292.1"
                     /translation="MTGRWLFSAPLDLAVFGGTAAISLLLVLAGPWLGAGRGAAPEWT
                     WVTGVLGVDVAHVWSTAFVVYLDPAEWRRRPALYAGTPLAAFAAGVALYAAGEAVFWR
                     ALAYLAVFHFIRQQYGWVMLYRARGGERGRVGRWLDGATVYAATLYPLIVWHTELPRA
                     FWWMKQGDFAAGLPAVAAAIAGWIYAALLASYAARAAWAAVARRPVAWGKHVVVAATA
                     ACWYAGIVATNTDYAFTVTNVFVHGVPYLVLVYLYARAASREPASSGGATARMLGDAP
                     RGRGHLRGIAVFCASLWAIAYLEELVWDRAIWHDRAWLFGAGGGVGHAALVVVPLLAV
                     PQLTHYVLDGFLWRRRANPRLGRLL"
     gene            1622..>1802
                     /locus_tag="VF516_41470"
     CDS             1622..>1802
                     /locus_tag="VF516_41470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114293.1"
                     /translation="MRSVVGQMCPIVTAKFCHCSYGRRSAIQVVNEVAMEREPALPQH
                     DGEGGADRAADSSVLG"
BASE COUNT          198 a          625 c          687 g          292 t
ORIGIN      
        1 gctacgtgtc ggcgacccac cagcgcggcc gcgatcgcgg cccgacggtg tggacctggt
       61 actacccgtt ccccggcgac gcccgcacgg cgcggaccca gctcgccggc gcgacctacg
      121 aggactgggc cgaggtcgtg ctcgccgacc tcgagcgcgc ccaccccgac gtccgcggcc
      181 tggtcgacca catcgaggtc gcgttctggg gccacggcat gatccggccg cgcaccggcg
      241 cgctgttcgg cggcggcggg atggccggcc ggatcgagcg cgcggcgccg gtcggccggc
      301 tgcacttcgc gcacaccgac ctgtcgggcc tcgcgctgtt cgaggaggcg ttcgatcacg
      361 gtctgcgcgc cgcgcgcgag gtcgccgagg cgctgacatg accgggcgct ggctgttctc
      421 cgcgccgctc gatctggcgg tgttcggcgg cacggcggcg atctcgctgc tgctggtgct
      481 cgcgggaccc tggctgggcg cgggccgcgg cgcggctccg gagtggacgt gggtgaccgg
      541 cgtgctgggg gtcgacgtcg cgcacgtgtg gtcgaccgcg ttcgtggtct acctcgatcc
      601 ggcggagtgg cggcgccggc cggcgctgta tgcgggtacg ccgctggcag cgtttgccgc
      661 cggggtcgcg ctgtacgcgg ccggcgaggc ggtgttctgg cgcgcgctgg cctacctcgc
      721 ggtgttccac ttcatccgcc agcagtacgg ctgggtgatg ctgtaccgcg cgcgcggcgg
      781 cgagcgcggc cgcgtcgggc gctggctcga cggcgcgacg gtgtacgctg cgacgctgta
      841 cccgctgatc gtgtggcaca ccgagctgcc gcgcgcgttc tggtggatga agcagggcga
      901 cttcgccgcc ggcctgcccg cggtcgccgc ggcgatcgcc ggctggatct acgccgcgct
      961 gctggcgagc tacgccgcgc gcgcggcctg ggcggcggtc gcccgccggc cggtggcgtg
     1021 gggcaagcac gtcgtggtcg ccgccaccgc ggcgtgctgg tacgccggca tcgtcgcgac
     1081 caacaccgac tacgcgttca ccgtgaccaa cgtgttcgtc cacggcgtgc cgtacctggt
     1141 gctggtctac ctctacgccc gggcggcgtc gcgcgagccg gcgtcgagcg gcggagcgac
     1201 cgcgcggatg ctgggcgatg ccccgcgcgg ccgcggtcat ctgcgtggaa tcgcagtgtt
     1261 ctgcgcatcg ctgtgggcga ttgcctatct cgaggagctg gtctgggatc gcgcgatctg
     1321 gcacgaccgc gcgtggctgt tcggggccgg cggcggggtc ggccacgcgg cgctcgtcgt
     1381 cgtgccgctg ctcgccgttc cccagctgac ccactacgtg ctcgacgggt tcctgtggcg
     1441 gcggcgggcc aatccgcggc tgggccggct gctctagaac agaagcttcg gagccgcgac
     1501 ccgcctgaca cggcgtagac aacgtgtaga cacagacgcg acggtcgccg cgtcgatcac
     1561 acatcggcca ctcgctcggg caaggtcggc gaggtgtcgt gttccgcctg ggatgaattt
     1621 tttgcgctcc gtcgtggggc agatgtgccc catcgtcaca gcgaaattct gtcattgttc
     1681 ttacggccgc cgatccgcta tccaggttgt taacgaggtg gcgatggagc gtgaaccggc
     1741 attgccccag cacgatggcg aaggcggcgc ggaccgcgcg gccgactcgt ccgtgctcgg
     1801 cg
//
LOCUS       DASVOR010002244         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_286179,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002244 DASVOR010000000
VERSION     DASVOR010002244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286179"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(375..779)
                     /locus_tag="VF516_41475"
     CDS             complement(375..779)
                     /locus_tag="VF516_41475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114294.1"
                     /translation="MARERHLTWILSLSFWTALGFLCGLQIWISMLDHGHTLWRVVTY
                     QVLIWNAWFVLTPVIGWLARRFPLVPPTARAVALHAIAALVIAPIHSVWWAMLLVVVR
                     PYDAMGTRDFGVAFRGVALSGLSQQIIMYAAC"
     gene            965..1189
                     /locus_tag="VF516_41480"
     CDS             965..1189
                     /locus_tag="VF516_41480"
                     /inference="COORDINATES: protein motif:HMM:NF021253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX8114295.1"
                     /translation="MAIEAVLQQVLKMPVEERAELVEHLIDSLEGDEVELSPEELAHL
                     DEAIADADRAVERRELIPAEAVLAQMRQIS"
BASE COUNT          273 a          572 c          622 g          238 t
ORIGIN      
        1 ggtcggggct gggacggagc gcggcggagc ggcggtggcc ggcgcggcga gcgcgagcag
       61 gagcggaagg acggtgggga gcctggggtc atgggtgcct cggtgccgcc agcgtggcgc
      121 gcgtgcgcaa cgaccgccgc cgggatgcag cgaacgggac cgcggccgcg gcgagccgga
      181 tcccggcgcg gcgaactgga tcaccagctc agcgcggggt gccgaacaga cgctcgaact
      241 cggcgcgccg cgtgcggctc agcttgacct gcgtgccgtc ggcgagcacc agcgtgcagt
      301 cgccgcggaa cagcgggtgg acctcgcgga tccggtcgac gtggacgatc gcgccgcgat
      361 ggcgtggtgc gcgatcagca cgcggcgtac atgatgatct gctgcgacag ccccgacagc
      421 gcgaccccgc ggaacgccac cccgaagtcg cgggtgccca tcgcgtcgta cggccgcacc
      481 acgaccagca gcatcgccca ccacaccgag tggatcggcg cgatcaccag cgccgcgatc
      541 gcatgcagcg cgaccgcgcg cgcggtcggc ggcaccagcg ggaaccggcg cgccagccag
      601 ccgatcaccg gggtcagcac gaaccaggcg ttccagatca gcacctggta agtgaccacg
      661 cgccacagcg tgtgcccgtg gtcgagcatg ctgatccaga tctgcagccc gcacagaaag
      721 ccgagcgcgg tccagaacga cagcgacagg atccaggtca ggtgccgctc gcgggccacg
      781 cgacgagtgt gccgcatgcc gcggccggat gggcagcggt gcagccaacg gcgccccggg
      841 cgcagcgaac gggaggtcgc gtcgtccatg ggaccgcgaa tgggctcgac gaccctgacg
      901 cccggcacgc cactcggtga accaggagcg tgtacggtct ccgttcatgc ggtacattcg
      961 ggtcatggcg atcgaagccg tgctccagca ggtcctcaag atgccggtcg aggaacgtgc
     1021 cgagctggtc gagcacctga tcgacagcct cgagggcgac gaggtcgagc tctctcccga
     1081 ggagctggcc cacctcgatg aggccatcgc cgacgccgat cgcgccgtcg agcgtcgtga
     1141 gctgatcccg gccgaagctg tgctcgcaca gatgcggcag atctcgtagc cggcgctccg
     1201 gcgcctctct tcacaccgcg cgcgatccgc gagttgcggg aggcggtgac atggtggcgc
     1261 aagaaccggc tcgaggtgcc tcggctgctc gatcacgaga tcgaacgcgc gttgcggcgc
     1321 atcgccgtat ttccgcggag cgccccggtc gttgcgcggt cgcgatgcgc gccgcgtggt
     1381 gcttcgccgt tcgggatatc tgctgttcta tcggatccgg ccgcgtctcg gtcgtatcga
     1441 ggtgatctcg gtactgcacg gccggcgggc cgatgttcca cgcccatagc aagcgattcg
     1501 gtcaccgatg cccggtgccc gcgacaggtg tctccgcctg tgacacccgc ggagacactc
     1561 ctcgctcagg cacgcagatg cgatgtcaca cacgattctt cgggatcttc ggatacggag
     1621 catcgtacga tgggcgcgat gttgcggtcg ctgcgcgcat gggggcggag gtgagcttcg
     1681 atcgatcgag ccgtggctcc ggcgg
//
LOCUS       DASVOR010002245        15040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_550891,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002245 DASVOR010000000
VERSION     DASVOR010002245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 15040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15040
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550891"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3291
                     /locus_tag="VF516_41485"
     CDS             <1..3291
                     /locus_tag="VF516_41485"
                     /inference="COORDINATES: protein motif:HMM:NF037816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase A-like domain-containing protein"
                     /protein_id="HEX8114296.1"
                     /translation="AATAGAAVTATGNFAAQIRAIAARQKQELTAKAATVKAELAASI
                     VAEKAALIVGFAQTLAKMQTDRDQALSDLNTHAETSRTQVRTAAQQEHAKLDQALARQ
                     QQAVRTAGETIATNAVTQATQQGDRVLQGSQQRAANARAVGEKWAGQFATLQGGGDTA
                     GDVRSKAADLATKLLDGANEARQTCVDHGTRFAEDLRKDAADTASRMPEKLADSRTHI
                     DKNRTDALTSIDDGIKSARDGITRSFDQSRQQVLDKQNGATQIYDQLAQGTAQQLDQG
                     VQQLSAKIDPMAEAIASHAEQAERDGQQYAVSDEVTATIHQRIASVVNDSLGKLTSAT
                     SQAQGALDTAKAQGQQGAQQQTQGVLGQLDTVGTGMKSSLTGKVAATTGKFDESASSA
                     VTNIATITPAADTAMGKVVTEGQTKWTQQLTDNVTKLSGGVDDALAHQDSQVGKLDTD
                     LGNQFNDAKAKQDKANQDQSWWSSAWDSITSFMGDVFSFLGGLVVGFFEAAWELIKGL
                     WDMLQTLWGILILIALIIIVVLVVIFVGWEALIIAGIILGLCFAAYYIYLAVTTPGLS
                     PYERGKLFGKALFNIVLGFAGVEFDWGELLNVAKWVPEAVELVRALGGLGRAIELVRA
                     LGGIGKAVEFLRAVGGAEQLLELAEAAGGIAQLVKLAEAAGGIAKLMELVEAAGGIQK
                     LVELAKAAGGIEKLLELAQAAGGIGKLLELAQAVGGIDKLLEMAQQAGGMAKLMELIT
                     AAGGIEKLLDLAKEVGGMPKLLEMAQQAGGMAKLLEMAQQAGGLPKLLELAEKAGGFD
                     KLVKLASDAGGFDKLLDLANKVGGIDKLLEMLRGPKIPDVATLERLLANAKIKDAAML
                     ERLLASTKIRDAQMLEDLLNHAKVPHGSVLEKLLGNAKLADGEALLRLLNNSKVTAAT
                     QIEKLLADAKILDSAQLERLLGHADIKDARMLQNLLRHPGVTDGAALEALLANPELSR
                     LPGSAGPLAAHDPAGHLNGTHIGKTDAELTARLGAEPGIPAASTFNSAAEAESAVAAT
                     LRQTAAQFWEWLAAGATGKLELTGSFSGGSVMLRGATASTPGSGITLVLKGSGGGNWF
                     ILTGFPIP"
     gene            3288..3662
                     /locus_tag="VF516_41490"
     CDS             3288..3662
                     /locus_tag="VF516_41490"
                     /inference="COORDINATES: protein motif:HMM:NF036906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent growth inhibition system
                     immunity protein"
                     /protein_id="HEX8114297.1"
                     /translation="MITELQRKTLRHFFAAYFHQDWRREADTPEQVISMYATDVNDLG
                     TIDTLVDGISAFIADHPDEDRLANALFREMGCYHSPKALGQSTREWLLHVAKTVKAER
                     PRVEQRAAEKRAVEDLDEGGEF"
     gene            3700..4218
                     /locus_tag="VF516_41495"
     CDS             3700..4218
                     /locus_tag="VF516_41495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114298.1"
                     /translation="MILAGMPRKAMSDVTVDIRVDLVLDRGVEELDEAAVAARIAAGD
                     TALLREARALDGMLRIGGGEVADELFSAVQRLCFEGAAALLADGAVVEYPYFSSNEHA
                     RLAAHGDTAVLSGSDVPESTFPRRDLIRALYACGARWLAVLDQIGRAPEANHLRPFAA
                     AAQSALAAAGLA"
     gene            4418..4972
                     /locus_tag="VF516_41500"
     CDS             4418..4972
                     /locus_tag="VF516_41500"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8114299.1"
                     /translation="MREAGLANADFSDMNLSEVDLREARLIGAHLRRADLYGSLLAGV
                     QLDRSDLTGANLAKANLDEASLVDALLRDAKVARTTFSGADLRRADFTGADLLKAYLR
                     DADLREAILACNLDRVSLTGARLAGARLTGATGLSSAIVEWIDIGEDEPQRWKAPLRW
                     PGWWRPLSGKRRSRPGDASDQGAA"
     gene            4969..5331
                     /locus_tag="VF516_41505"
     CDS             4969..5331
                     /locus_tag="VF516_41505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114300.1"
                     /translation="MSHEQRESRHPRRSGSRPRGPGSGSAANELPLVDELIPFLLARS
                     GQALDSPQKKQQLRQEGQLEGRLTEARAALRQVLVGRQLTPSPDDDARIDACTDLTIL
                     ERWLDRAITATRVSDVLR"
     gene            5741..6238
                     /locus_tag="VF516_41510"
     CDS             5741..6238
                     /locus_tag="VF516_41510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114301.1"
                     /translation="MNDSDAADRTTRRGPNQRGHAPGGKSRVSVDSFRRDPMYPQVER
                     AVTAILASSKVVAPVDVIVRLGWLSAADLENWRFGRVPYLERVIRCNLTRLSRFLRIL
                     GFHCHDLNLTASQTAYVKWGKGRRTALRFTKTGDPPLERVYARHFVWPGKGPFHPPRP
                     RDDLA"
     gene            6509..7549
                     /gene="had"
                     /locus_tag="VF516_41515"
     CDS             6509..7549
                     /gene="had"
                     /locus_tag="VF516_41515"
                     /EC_number="1.1.1.368"
                     /inference="COORDINATES: protein motif:HMM:TIGR03201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxycyclohex-1-ene-1-carbonyl-CoA
                     dehydrogenase"
                     /protein_id="HEX8114302.1"
                     /translation="MLTASGAPLSRRSISIAAPGADEAIVEVEACGLCHTDLGFASGA
                     VAPKHPLPLVLGHEIVGKVIAAGGSYEALIGRKVLVPTVMGCGRCAFCQAGRGNACPA
                     QKMPGNDIDGGFASHLVVPAWPLVPVDDAPGTVDTRELAVVADAVSTAYQAVRRAALA
                     RGDAAFVIGAGGVGGYAIQIAKAMGARVVACDVSADKLALVRNHGAEHTIDVSGRAPK
                     DVRKEVQALAHEWAIPSLAHRIFECSGTTQGQMLAFTLLGAAATMVQVGYTAKPVEVR
                     LSNLMAFDATVHGTWGCPPEAYPDVLRMIYAGEVVLAPFVEHAAMSRINEIFDDMTHH
                     RLARRVIFDPRR"
     gene            7584..8702
                     /gene="oah"
                     /locus_tag="VF516_41520"
     CDS             7584..8702
                     /gene="oah"
                     /locus_tag="VF516_41520"
                     /EC_number="3.7.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018807 -
                     6-hydroxycyclohex-1-ene-1-carboxyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase"
                     /protein_id="HEX8114303.1"
                     /translation="MTDLKNHDLIPEHEYRHIRYEERPVLDRNGKAVEGLHQVWLSLD
                     NEEQLNSYTTAAVKDVILGLRRASADRRAVCVVLTAVGTRSFCTGGNTAEYASYYAGK
                     PLEYLQYMRLFNDMVTGILLCDKPVICRVNGMRIGGGQEIGMACDFSIAGDHARFGQA
                     GPVHGSAPDGGSTDFLHLYVGFAYAAESLSLCEPWSAHKAMRLGMLNEIVPVHKRPDG
                     TFIANPLVITDRYADELGRIVFGEAKSGAALDAGKAEAKTCTIDLSRLDAAVHRLATK
                     LLHTFPDCTRKTLESLRKKKLEHWFQNAETSRSWLSLNMNTEASAGFAAFHYGDKAER
                     EIDFVLLRKRLAEGAPFDRKLVLEVVPASARERMKAAP"
     gene            8699..9517
                     /locus_tag="VF516_41525"
     CDS             8699..9517
                     /locus_tag="VF516_41525"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEX8114304.1"
                     /translation="MTGAAEPGYRTITASLVEDGSVLSLVLNQPKANVLSIAMMAELR
                     AALAAHRAHRKLRLVIVRGAGKHFSFGASVPEHRRDSAEAMLAAFHTLVRDIASYPVP
                     VAALVLGQCLGGAFELVLTCHFVFAAPSAVFACPEIKLGVVPPVLAVLGHHRLGGPLA
                     ERMVMTGAELTAHQAHDRGLLAALLPESFDGVDTAALPFVLDWYRATLAPLSAFSLRE
                     ATHAARTGSGLLASLGAPLDAAERRYIELLLPSRDGNEGIEAFLARRAPAWVDA"
     gene            9514..10251
                     /locus_tag="VF516_41530"
     CDS             9514..10251
                     /locus_tag="VF516_41530"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8114305.1"
                     /translation="MTLAARRDDRRADVLRGAVVSIARHGYHGMSMRDLAKATGRSLA
                     SFYHLFRSKEDILFEIQSQAFEQLVATVELALAEVEDPIGRLQIFVLNHVRYFAEQPD
                     VMRVLIHEAAALPRPRRAQIRALKQRYFEIARDVVRAILERDPGAAGAPDPGEVERIT
                     YCVFGMLNWIYGWYQPARHGSPEQLARTIQHLALRGITAAPRDLALADAPRRVRPDDA
                     SWDAIASALDAIERPPLLRTPERGAPR"
     gene            10248..11423
                     /locus_tag="VF516_41535"
     CDS             10248..11423
                     /locus_tag="VF516_41535"
                     /inference="COORDINATES: protein motif:HMM:NF017828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HEX8114306.1"
                     /translation="MIDPAAPVIDHARSLVEDRSLRCVAAWKQAHPGGLAIGHMPIYV
                     PRPLLEAIDCLPVAIFGGGEIDIIRGDSYFQSYICHIPRSTVELALSGDLAVLDGMLF
                     PAICDVIRNLGGMWKMLFPDAYTAYLDLPQNFDPAVGGKFYALELRRIARELEARGAP
                     PLRPDALRVAIAAESKRREAIEELDAIRRTEPWRLRASEAYLVVRAGAALPAAEHERL
                     VREFVAAIKQRSARAVDNVRVVLVGSFCEQPPFELIRALERAGCDIVDDDFQLGMRMI
                     DGPIEVGPDEDPVDALARGYLEQGRDTASRYIGDLVKGAALIRRVKECHADGVIFAAA
                     SFCDPALLDQPMLESALTRANVPHTNFKFSENNGQFQVIREQAGAFSDAVKLWGAAS"
     gene            11420..12769
                     /gene="bcrB"
                     /locus_tag="VF516_41540"
     CDS             11420..12769
                     /gene="bcrB"
                     /locus_tag="VF516_41540"
                     /EC_number="1.3.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR02260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018522 - benzoyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoyl-CoA reductase subunit B"
                     /protein_id="HEX8114307.1"
                     /translation="MTTAEASKDRSQLRQKRMIAEHFEKLARAKAEGNKVVYTFVPGN
                     VTELLGALDLLPVLPEINALQSGMRKRSGAYIAEAEKAGHSEDVCTYVKCDIGMMKSG
                     NIGPTGTKLPDPDLLLLSYTGCFTFMKWFELLKQEYACPVVMLHVPYQTDGKITPEMR
                     AYVVEQLQREVIPALEKLAGKKLDLNELSRRLALSARAEEDLVHVWESARHRPSPIDG
                     YFGGVYYVGPIFSSFRGSEDAALYYQELREEVDERVRDQRGPMTPDGEMPSEKYRLVV
                     EGPPNWTSFHDFWRMFSKEGASVVASTYTRVGGLYDTGFRHDPARPLESLAEYCLGCY
                     TNLGLPTRLDLLERYVRTYQADGFLINSVKSCNSFSVGQLLMLRKLEERTGVPGGFIE
                     SDLVDPRYFGKANIENRVQSFLQVIDARRRRGGAPEPPAEPDRRRVALPVLDTAREP"
     gene            12766..14127
                     /locus_tag="VF516_41545"
     CDS             12766..14127
                     /locus_tag="VF516_41545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX8114308.1"
                     /translation="MKCVVGIDLGSTTTKAVLLDEDGKMLGRGITNSRSNYEVACAVA
                     RIEALTSARFDLLERELARAGTGTKVWVGRLQDAFRLELFMGEFEDLRSTILANLDTV
                     RKGDQRTALDRAATLVLDAVQTTAPRLFLGGAPRKSEFFRDLAGALFVAEAEERARVG
                     DVPFEPLVGLYDRAILEVETRLRPRDTRALLHRAVARLGEVEGAAAIVPMLARAVDTA
                     TRQDIDEVAVVGTGYGRQTLPFPKSCVRSEILCHGRGAHRVFPRTRTVLDIGGQDTKA
                     IQVDDRGVVTSFQMNDRCAAGCGRYLGYIADELNLGLHELGPLALTSKKIIRINSTCT
                     VFATAELRERLGLGQRREDILAGLHHAIILRAMSLLARSGGVKNEFTFTGGVCNNAMA
                     TKVLRDLVNANYGEDIVLNVHPDSIYMGGLGAAMFALEDFRTGQVDLLPSFVTDARSE
                     IRS"
     gene            14124..15014
                     /locus_tag="VF516_41550"
     CDS             14124..15014
                     /locus_tag="VF516_41550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEX8114309.1"
                     /translation="MSTRQHLSAGIDAGSSAVKVAIVRSHAGEAPELLATTVQRIRRR
                     DVRDVVTAAFAEACATAKVEAAVFDYVASTGDGDAVGFATGHFYGMTTHARGALYLVP
                     TARAALDIGALHARAIRMDSRGKVLGHRMTSQCASGTGQFLENIARYLGVPIEDVGRM
                     SRESAQAEEVSSVCAVLAETDVINMVSRGIRTGEILRGIHLSIAGRLVRLLRSAGADG
                     VVALTGGLALDDGLVAVLAELLASEPSKNKKPVTPLTVVAHPMAALAGAIGAAILGAY
                     RFDQLEQRGRTVGASASGPA"
BASE COUNT         2482 a         5161 c         5108 g         2289 t
ORIGIN      
        1 gccgccaccg ccggcgctgc cgtgaccgcg accggcaact tcgcggcgca gatccgcgcg
       61 atcgcggcgc gccagaagca ggagctgacg gcgaaggcgg cgaccgtgaa ggccgagctc
      121 gccgcgtcga tcgtcgccga gaaggcggcg ctgatcgtcg gcttcgcgca gacgctcgcg
      181 aagatgcaga ccgaccgcga tcaggcgctg tcggacctga acacgcacgc cgagacgtcg
      241 cgcacgcagg tgcgcaccgc cgcgcagcag gagcacgcga agctcgatca ggcgctcgcg
      301 cggcagcagc aagcggtgcg caccgccggc gagacgatcg cgaccaacgc cgtcacgcag
      361 gcgacgcagc agggcgaccg cgtgctgcag ggctcgcagc agcgggccgc aaacgcgcgc
      421 gcggtcggcg agaagtgggc ggggcagttc gcgacgctcc agggcggcgg ggacaccgcg
      481 ggcgacgtgc gctcgaaggc cgcggatctc gcgaccaagc tgctcgacgg tgcgaacgag
      541 gcacggcaga cctgcgtcga tcacggcacc cggttcgccg aggacctgcg caaggacgcg
      601 gccgacaccg cgtcgcgcat gcccgagaag ctcgccgact cgcgcaccca catcgacaag
      661 aaccggaccg acgcgctcac ctcgatcgac gacggcatca agagcgcgcg cgatggcatc
      721 accaggtcgt tcgaccagtc gaggcagcag gtgctcgaca agcagaacgg cgcgacgcag
      781 atctacgacc agctcgcgca gggcaccgcg cagcagctcg atcagggcgt ccagcagctg
      841 tctgcgaaga tcgatccgat ggcggaggcg atcgcctcgc acgccgagca ggccgagcgc
      901 gacggccagc agtacgccgt ctccgacgag gtcaccgcga cgatccatca gcggatcgcg
      961 agcgtcgtga acgactcgct cggcaagctc acgtcggcga ccagccaggc gcagggcgcg
     1021 ctcgacacgg cgaaggccca gggccagcaa ggcgcgcagc agcagaccca gggcgtgctc
     1081 ggccagctcg acaccgtcgg cacgggcatg aagtcgagcc tgaccggcaa ggtcgcggcg
     1141 acgaccggca agttcgacga gtccgcgtcg tcggcggtga ccaacatcgc gacgatcacg
     1201 cccgcggccg acaccgcgat gggcaaggtc gtcacggagg gtcagacgaa gtggacgcag
     1261 cagctcacgg ataacgtcac caagctctcg ggcggcgtcg acgacgcgct cgcccaccag
     1321 gactcgcagg tcggaaagct cgacaccgat ctcggcaacc agttcaacga cgcgaaggcc
     1381 aagcaggaca aggcgaatca ggaccagagc tggtggtcga gcgcgtggga ctcgatcacc
     1441 agcttcatgg gcgacgtgtt ctcgttcctc ggcggcctgg tcgtcgggtt cttcgaggcc
     1501 gcgtgggagc tgatcaaggg cctctgggac atgctccaga cgctgtgggg catcctgatc
     1561 ctcatcgcgc tgatcatcat cgtcgtcctg gtcgtgatct tcgtcggctg ggaggcgctg
     1621 atcatcgccg ggatcatcct cggcctgtgc ttcgccgcgt actacatcta cctcgcagtc
     1681 accacgcctg gcctgtcgcc gtacgagcgc ggcaagctgt tcggcaaggc gctgttcaac
     1741 atcgtgctcg gcttcgcggg cgtcgagttc gactggggcg agctgctgaa cgtcgcgaag
     1801 tgggtgccgg aggcggtgga gctggtgcgc gcgctcggcg gcctcgggcg cgcgatcgag
     1861 ctcgtgcgtg cgctcggcgg catcggcaag gcggtcgagt tcctgcgggc agtcggcggc
     1921 gccgagcagc tcctggagct ggccgaggcg gccggcggca tcgcgcagct ggtcaagctc
     1981 gccgaggcgg cgggcggcat cgccaagctg atggagctgg tcgaggcggc cggcggcatc
     2041 cagaagctcg tcgagctggc caaggccgcg ggcggcatcg agaagctcct cgagctcgcc
     2101 caggccgcgg gcggcatcgg caagctgctg gagctggcgc aggccgtcgg cgggatcgac
     2161 aagctgctcg agatggccca gcaggccggc ggcatggcca agctgatgga gctgatcacc
     2221 gccgccggcg ggatcgagaa gctgctcgac ctcgccaagg aggtcggcgg catgccgaag
     2281 ctcctcgaga tggcccagca ggccggcggc atggccaagc tgctcgagat ggcccagcag
     2341 gccggcggcc taccgaagct gcttgagctc gcagagaagg ccggcggctt cgacaagctt
     2401 gttaagctcg cgagcgacgc gggcggcttc gacaagctcc tcgatctcgc gaacaaggtc
     2461 ggtgggatcg acaagctgct cgaaatgctc cgcggcccca agatcccgga cgtcgccacg
     2521 ctcgaacgcc tcctcgcgaa cgccaagatc aaggacgccg cgatgctcga gcggctcctc
     2581 gcgagcacca agatacgcga cgcccagatg ctcgaggatc tgctcaacca cgccaaggtc
     2641 cctcacggct cggtgctgga gaagctgctc ggcaacgcca agctcgccga tggcgaagcg
     2701 ctgctgcggc tgctcaataa ctccaaggtg accgcggcga cccagatcga gaagctgctc
     2761 gccgacgcca agatcctcga ttctgcgcaa ctcgagcgcc tcctgggtca cgccgacatc
     2821 aaggacgccc ggatgctgca gaatcttctc aggcaccccg gcgtcactga cggtgccgcg
     2881 ctggaggcgc tcctcgccaa cccggaattg tcgcggctac cgggcagcgc aggccccctc
     2941 gcggcacacg atccggccgg ccacctgaac ggtacccaca tcgggaaaac ggacgccgag
     3001 ctcacggcgc ggctcggcgc cgaaccgggg attcctgctg cctccacgtt caactccgcc
     3061 gcggaagcgg agtccgcggt cgccgccaca ctccgccaga ccgccgcgca attctgggaa
     3121 tggctcgcag ccggcgcaac aggtaagctc gagctcacgg gatcgttctc gggcggcagc
     3181 gtgatgctgc gaggcgcaac cgcatcgacg ccgggcagtg gcatcacgct cgtcttgaag
     3241 ggttcgggtg gcggaaactg gttcattctg acaggcttcc caatcccatg atcaccgagc
     3301 tgcagcgcaa gaccttgcgt cacttcttcg ccgcctactt ccatcaggac tggcgccgag
     3361 aagcagatac gccggagcag gtgatctcga tgtacgccac tgacgtgaac gacctcggaa
     3421 cgatcgatac gctcgtcgat ggcatcagtg cgttcatcgc cgatcatccg gacgaggaca
     3481 ggctcgcgaa tgcgttgttc cgtgagatgg gctgttacca ttcgcccaag gcgcttgggc
     3541 agtcgacccg agagtggcta ttgcatgtcg cgaagacggt caaggcggag cggccccgcg
     3601 tcgagcaacg cgccgcagag aagcgggctg tggaagatct cgacgaaggc ggcgaattct
     3661 agcccggtct cacgatcctg tacctctact cagagggaca tgatcctggc ggggatgccg
     3721 cgcaaagcca tgagtgacgt gacggtggac attcgcgtcg atctggtcct cgaccgcgga
     3781 gtcgaagagc tggacgaagc cgccgtcgcc gcgcggatcg cggccggcga tacagccttg
     3841 ctgcgcgagg cgcgcgcgct cgacggtatg ctccgcatcg gcggcggcga ggtcgctgac
     3901 gagctgttct ccgccgtgca acggctgtgc ttcgagggtg ccgcggcgct gctcgccgat
     3961 ggcgccgtcg tcgagtaccc gtacttctcg agcaacgagc acgcgcggct cgcggcgcac
     4021 ggtgacaccg ccgtgctctc cggaagcgac gtgccggagt ccacgttccc gcgacgagac
     4081 ctgatccgcg cgctctacgc gtgcggcgct cgctggttgg cggtgctcga ccagatcggt
     4141 cgcgctccgg aggccaacca tctccgcccg tttgcggcgg cggcgcagtc cgccctggct
     4201 gccgccggcc tcgcgtagtg tgtgaacgac gagctcgcgc cggttgcgcc cgccgatcac
     4261 cgttaccccg gtgaagcggc cgagctcccg gacggcgacg gcgtagcatc gacaggtacc
     4321 catgatcggc ggttagcagc gaaaacagct ggagcagcgc atcgaacgtc accagatctg
     4381 gctggagtcg aacggcaccg acggcgagca gctcgacctg cgcgaggcag gtctcgcgaa
     4441 tgcggacttc tcggacatga acctcagcga ggtcgatctg cgcgaggctc ggctgatcgg
     4501 tgctcatctc aggcgcgcag acctctacgg aagcttgctc gccggtgttc agctcgaccg
     4561 cagcgatctg accggggcca atctggccaa ggcgaacctc gacgaggcga gcctcgtcga
     4621 cgcattgcta cgtgacgcca aggtagcgcg cacgacgttc tccggcgcgg atctgcgccg
     4681 cgccgacttc accggggccg acttgctcaa ggcgtatctc cgcgacgctg acctccgcga
     4741 agcgatcctg gcctgcaatc tcgatcgagt cagtctcacc ggcgcgcgac tcgccggggc
     4801 acgcctcacc ggcgcgacgg gcctgtcatc agcgatcgtc gaatggatcg acatcgggga
     4861 agacgagcca cagcggtgga aggcgccgct gcgatggcct ggttggtgga ggccgctgag
     4921 cggtaaaaga cgatcccgac caggtgatgc gagcgatcaa ggcgctgctt gagccatgag
     4981 cagcgtgaaa gtagacatcc gcgtcgatct ggttctcgac cgcgggggcc aggatccggg
     5041 agcgcagcca acgagctccc cctcgtcgac gagctgatcc cctttctgct cgcccggtcc
     5101 ggtcaggcgc tggacagccc ccagaagaag cagcagctgc ggcaggaagg tcagctggaa
     5161 ggtcggctga ccgaggcgcg tgctgccctg cgccaggtgc tcgtgggccg tcagctcaca
     5221 ccgagcccgg acgacgacgc gcgcatcgac gcctgcaccg acctcacgat cttggagcgc
     5281 tggctcgacc gagccatcac tgcgacccgc gtctcggacg tgctcaggta gcacgcctcc
     5341 cgagcgtgcc ggcgatcggc agcgagcacg cgcgacacgc gcaggaacgc ctccggtcgg
     5401 ccgagccatc ggcgaaggac ttcgcaaaga ccacactggc cgaaaaatcg ccccggaaac
     5461 tgactcaact cactgaaggc gctcgggaaa tcatcactgc ggaaaagtgc tgagcgccta
     5521 agaagctgaa atcagcgact cttgacggga tttaggttcc tgtccgtaac agggtcccgg
     5581 ttcgagtccc ggcgctcgca cgcgtctttc gaggacttgg aaagtcctga aaggcttctc
     5641 gaaagtcacg aaaggctttc gcgaagcttc gtatcgccgg ccgtcactcg gtgcggcgag
     5701 gttggccgcg cgcggtcgac aacagtggca gagttgccgc gtgaacgact ccgacgctgc
     5761 cgaccggacc accagacgag gaccgaatca acgcgggcat gcgcccgggg gcaagtcgcg
     5821 tgtgtccgtc gattcgttcc gccgcgatcc gatgtacccg caggtagagc gcgcggtgac
     5881 tgcgattctc gcaagcagca aggtcgtcgc gccggtcgat gtgatcgtcc ggctggggtg
     5941 gctgtccgcc gccgatctcg agaactggcg gttcgggcgc gtgccgtacc tcgagcgcgt
     6001 gatccgctgc aacctgacgc ggctcagccg tttccttcgc atcctcggct ttcattgtca
     6061 tgatctgaac ctcaccgcgt cgcagactgc gtacgtgaag tggggcaagg gccgacgcac
     6121 agcgttgcgc ttcaccaaga ccggcgatcc tccgctcgag cgagtctacg cacgccattt
     6181 cgtctggccc ggcaagggtc cgtttcatcc accgcgtccg agggatgatc tcgcataagg
     6241 gctcgggcgc ctggcggcgc gccggccgcg aaacgcgcac gccttgcgtg cgagcggtgg
     6301 aacgtccgcg atcgatcgca ccgattgcac gcgttcgaga tcgatcgcgc cgcgacgacc
     6361 acctgtgatc gatccgcagt cggctgagaa atcgcggatc acacaagtca ttcagcggca
     6421 cgccgttttg cgagatccgg aacgaacgtt cgtccctggt ctggcttggc acatgcattt
     6481 gcaaactgcg atgcaagtcg acgcgtttgt gctcactgcg agtggtgcgc cgctgtctcg
     6541 tcgttcgatc tcgatcgcgg cgcccggcgc ggacgaggcc atcgtcgagg tcgaggcgtg
     6601 cggtctgtgc cacaccgatc tggggttcgc gagcggcgcc gtcgcgccca agcatccact
     6661 gccgctcgtg ctcggccacg agatcgtcgg caaggtcatc gctgcgggcg gcagctacga
     6721 ggcgctgatc ggccgcaagg tgctggtgcc caccgtgatg ggctgcggcc gctgcgcgtt
     6781 ctgccaggcg ggccgaggca acgcgtgccc tgcgcagaag atgccgggca acgacatcga
     6841 cggcgggttc gcctcgcacc tggtcgtgcc ggcgtggccg ctcgtcccgg tcgatgacgc
     6901 acccggcacg gtcgacacgc gcgagctcgc cgtggtcgcg gacgccgtgt cgacggcgta
     6961 ccaggccgtg cgtcgcgcgg cgctcgcccg gggagacgcc gcgttcgtga tcggcgcggg
     7021 cggcgtcggc ggctatgcga tccagatcgc gaaggcgatg ggcgcgcgcg tcgtcgcgtg
     7081 cgacgtcagc gccgacaagc tcgccctggt ccgcaaccac ggcgccgagc acacaatcga
     7141 tgtctccggt cgcgcgccga aggacgtgcg caaggaggtc caggcgctgg cgcacgagtg
     7201 ggccatcccg tcgctcgcgc accggatctt cgagtgtagc ggcacgacgc aaggccagat
     7261 gctggcgttc acgctgctcg gcgccgcggc gacgatggtg caggtcggct acacggccaa
     7321 gccggtcgag gtccggctgt cgaacctgat ggcgttcgac gcgaccgtgc acggcacctg
     7381 gggctgtcca cccgaggcct accccgacgt gctccgcatg atctacgccg gcgaggtcgt
     7441 gctcgcgccg ttcgtcgagc acgcggcgat gtcgcgcatc aacgagatct tcgacgacat
     7501 gacccatcac cgcctcgcca ggcgcgtgat cttcgacccc cgccgctaga gctcaccacc
     7561 ttgaccattg caggagcgac acgatgaccg atctcaagaa tcacgacctc attccggagc
     7621 acgagtatcg ccatatccgt tacgaggagc gaccggtgct cgatcgcaac ggcaaggcag
     7681 tcgaggggtt gcaccaggta tggttgtcgc tcgacaacga ggaacagctc aactcgtaca
     7741 ccacggcggc ggtcaaggac gtgatcctcg ggctgcgccg cgcgagcgcc gatcgcagag
     7801 cggtgtgcgt ggtgctcacc gcggtcggga cgcggtcgtt ctgcaccggc ggcaacacgg
     7861 ccgagtacgc cagctactac gcaggcaaac cgctcgagta tctgcagtac atgcggctgt
     7921 tcaacgacat ggtcacgggg atcttgctgt gcgacaagcc ggtgatctgc cgcgtcaacg
     7981 gcatgcggat cggcggcggg caggagatcg gcatggcgtg cgacttctcg atcgcgggcg
     8041 atcatgcgcg gttcggccag gccggtccgg tccacggctc ggcgccggac ggcggctcca
     8101 cggacttcct gcatctctac gtcggctttg catacgccgc cgagtcgctg tcgctgtgcg
     8161 agccgtggtc ggcgcacaag gcgatgcgcc tgggcatgct caacgagatc gtgcccgtcc
     8221 acaagcgccc cgacgggacg ttcatcgcca acccgctggt catcaccgat cgctacgccg
     8281 acgagctcgg ccggatcgtg ttcggcgagg cgaagtccgg cgccgcgctc gatgcgggca
     8341 aggccgaggc caagacgtgc acgatcgacc tgagccggct cgacgcggcc gtccaccggc
     8401 tcgcgaccaa gctgctccac accttccccg actgcacgcg caagacgctc gagagcctgc
     8461 gcaagaagaa gctcgagcac tggttccaga acgccgagac cagccggtct tggctgtcgc
     8521 tcaacatgaa caccgaggcg tccgcggggt tcgcggcgtt ccattacggc gacaaggcag
     8581 agcgcgagat cgacttcgtg ctgctccgca agcggctcgc cgagggtgcg ccgttcgacc
     8641 gcaagctcgt gctcgaggtc gtcccggcct cggcccgcga gcgcatgaag gccgcgccat
     8701 gaccggcgcg gccgagcccg gctaccggac gatcacggcc tcgctcgtcg aggacggcag
     8761 cgtgctctcg ctcgtgctga accagcccaa ggcgaacgtg ctgtcgatcg caatgatggc
     8821 cgagctgcgc gccgcgctcg cggcgcaccg cgctcatcgc aagctccgcc tggtgatcgt
     8881 gcgcggcgcc ggcaagcact tctcgtttgg cgcttcggta cccgagcatc gccgcgactc
     8941 ggcagaggcc atgctcgccg cgttccatac gctggtgcga gacatcgcga gctacccggt
     9001 cccggtcgcg gcgctcgtgc tcggccagtg cctgggcggc gcgttcgagc tcgtgctcac
     9061 gtgccacttc gtgttcgccg cgccgtcggc ggtgttcgcg tgcccggaga tcaagctcgg
     9121 cgtcgtgccc cccgtgctcg ccgtgctcgg tcaccatcgc ctcggtgggc cgctggccga
     9181 gcgcatggtg atgaccggcg cggagctcac cgcgcaccag gcgcacgacc gcggcctgct
     9241 cgccgcgctg ctgcccgagt cgttcgacgg ggtcgacacc gcggcgctgc cgttcgtgct
     9301 cgactggtat cgcgcgacgc tggcgccgct gtcggcgttt tcgctgcgcg aggcgaccca
     9361 cgccgcgcgc accggatccg gcctgctcgc gagcctgggc gccccgctcg acgctgcgga
     9421 gcgccgctac atcgagctgc tgctgccgag ccgcgatggc aacgagggga tcgaggcgtt
     9481 cctcgcccgg cgcgctccgg cgtgggtgga cgcatgacgc tcgccgcccg tcgtgacgat
     9541 cgccgcgcgg acgtgctccg cggcgccgtg gtgtcgatcg ctcgccacgg ctatcacggg
     9601 atgtcgatgc gcgacctcgc gaaggcgacc ggcaggagcc tcgccagctt ctaccacctg
     9661 ttccgctcga aggaggacat tctgttcgag atccagtccc aggcgttcga gcagctggtc
     9721 gcaacagtgg agctcgcgct cgcggaggtc gaggatccga tcggccggct acagatcttc
     9781 gtgctcaacc atgtgcgcta cttcgccgag cagcccgacg tgatgcgtgt gctcatccac
     9841 gaggccgcgg cgctgcccag gccgcgccgc gcgcagatcc gcgcgctcaa gcagcgctac
     9901 ttcgagatcg cgcgcgacgt cgtgcgcgcc atcctcgagc gcgatccagg cgcggccggc
     9961 gcgcccgatc cgggagaggt cgagcgcatc acctactgcg tgttcggaat gctcaactgg
    10021 atctacggct ggtaccagcc ggcgcggcac ggctcccccg agcaactcgc gcgcacgatc
    10081 cagcacctcg cgctgcgcgg catcaccgcc gcaccgcgcg acctcgcgct ggccgatgcc
    10141 ccccggcgcg tccggccgga cgacgcgagc tgggacgcca tcgcgagcgc gctcgatgcg
    10201 atcgagcgcc cgccactgct ccgcaccccc gagagaggag ccccgagatg atcgatcctg
    10261 ctgcgccggt gatcgaccac gcccgttcgc tcgtcgagga tcgctcgctg cgctgcgtcg
    10321 ctgcgtggaa gcaagcacac cccggtgggc tcgcgatcgg ccacatgccg atctacgtcc
    10381 cccggccgct gctcgaggcg atcgactgtc tccccgtcgc gatcttcggc ggtggcgaga
    10441 tcgacatcat ccggggcgac tcgtacttcc agtcgtacat ctgccacatc ccgcgcagca
    10501 ccgtggagct cgccctgtcc ggtgatctgg ccgttctcga cggcatgctg ttcccggcga
    10561 tctgcgacgt gatccgcaac ctgggcggca tgtggaagat gctgttcccc gacgcgtaca
    10621 cggcctacct cgacctgccg cagaacttcg atccggccgt cggcggcaag ttctacgccc
    10681 tcgaactccg ccgcatcgcc agggagctcg aggcgcgggg cgcaccgccg ctccgtcccg
    10741 acgcgctgcg cgtggcgatc gccgccgagt cgaagcgccg cgaagccatc gaggagctcg
    10801 acgccatccg gcgtaccgag ccctggcggc tccgggcgag cgaggcctac ctcgtcgtgc
    10861 gcgcaggtgc cgcgctgccc gcggccgagc acgagcgcct ggtccgcgag ttcgtcgccg
    10921 ccatcaagca gcgctccgcg cgcgctgtcg acaacgtccg ggtggtgctg gtcggctcgt
    10981 tctgcgagca gccgccgttc gagctgatcc gtgcgctgga gcgcgccggc tgcgacatcg
    11041 tcgatgatga tttccagctc ggaatgcgga tgatcgacgg tccgatcgag gtcggccccg
    11101 acgaggaccc ggtggacgcg ctggcgcgcg gctacctcga gcagggtcgg gacacggcgt
    11161 cgcgctacat cggggatctc gtcaaggggg cggcgctgat ccgccgcgtc aaggaatgtc
    11221 acgccgacgg cgtgatcttt gccgctgcgt cgttctgcga tcccgcgctg ctcgatcagc
    11281 cgatgctcga gagcgcgttg acccgcgcca acgtgccaca caccaacttc aagttctcgg
    11341 agaacaacgg acagttccag gtcattcgcg agcaagccgg cgcgttctcc gacgccgtca
    11401 agctctgggg ggccgcatca tgaccactgc cgaggcctcg aaggatcgca gccagctgcg
    11461 ccagaagcgc atgatcgccg agcacttcga gaagctcgcg cgcgccaagg ccgagggcaa
    11521 caaggtcgtc tacacgttcg tccccggaaa cgtgaccgag ctgctcggcg cgctcgatct
    11581 cctgccggtg cttcccgaga tcaacgcgct ccagagtggc atgcgcaagc gctccggggc
    11641 ctacatcgcg gaggccgaga aggccggaca ctccgaggac gtctgcacgt atgtcaagtg
    11701 cgacatcggc atgatgaaga gcggcaacat cggtccgacg ggcacgaagc tcccggaccc
    11761 ggatctgctg ctgctctcgt ataccggctg cttcacgttc atgaagtggt tcgagctgct
    11821 caagcaggag tacgcctgcc ccgtcgtcat gctccacgtc ccctatcaga cggacgggaa
    11881 gatcacgccg gagatgcgag cctacgtcgt cgagcagctg caacgggaag tcatcccggc
    11941 gctcgagaag ctggccggca agaagctcga cctgaacgag ctgtcgcgcc ggctcgcgct
    12001 gtcggcccgc gccgaggagg atctggtcca cgtgtgggag tccgctcgtc accggccgtc
    12061 gccgatcgac ggatacttcg gcggcgtcta ctacgtcggg ccgatcttct cgtcgtttcg
    12121 cggcagcgaa gacgccgcgc tctactacca ggagctgcgc gaggaggtcg acgagcgggt
    12181 ccgcgaccag cgcggcccga tgacgccgga cggcgagatg cccagcgaaa agtatcgact
    12241 cgtcgtcgag ggtccaccca actggacctc gttccacgat ttctggcgga tgttcagcaa
    12301 ggagggcgca tcggtggtcg cgagcacgta tacgcgcgtc ggcggcctct acgacacggg
    12361 gttccgccac gatccagcgc gcccgctgga gagcctcgca gagtactgcc tcggttgcta
    12421 cacgaacctg gggttgccca cgcgcctcga tctgctcgag cgctacgtca ggacttatca
    12481 ggctgatggc tttctcatta atagcgttaa gtcctgtaat tcgttcagcg tggggcagct
    12541 cctcatgctg cgcaagctcg aggagcgcac gggcgtgccg ggcggtttca tcgagtccga
    12601 tctggtggac ccgcgctact tcggcaaggc aaacatcgag aaccgcgtgc agagcttcct
    12661 gcaggtcatc gatgcccggc gtcgccgagg cggcgcgccc gaaccgcccg ccgagccgga
    12721 ccggcgccgg gtcgccctcc ccgtcctcga cacggcgagg gagccatgaa gtgcgtcgtc
    12781 ggcatcgacc tgggctcgac cacgacgaag gcggtcctcc tcgacgaaga cggcaagatg
    12841 ctcggccgcg gtatcaccaa cagtcgcagc aactacgagg tcgcctgtgc cgtcgcgcgc
    12901 atcgaggcgc tcacctcggc gcgcttcgat ctgctcgagc gcgagctcgc gcgggccggc
    12961 acgggaacga aggtgtgggt cggccggctg caggacgcgt tccggctcga gctgttcatg
    13021 ggcgagttcg aggatctgcg gtccacgatc ctcgccaacc tcgacaccgt aaggaagggc
    13081 gaccagcgga ccgcactcga ccgcgcggca acgctcgtcc tcgacgccgt ccagacgacc
    13141 gcgccccggc tgttcctcgg tggggcgccg cgcaagagcg agttcttccg cgatctcgcc
    13201 ggggcgctgt tcgtcgcgga ggcagaggag cgtgcccgcg tcggtgacgt cccgttcgag
    13261 ccgctcgtcg gcctctacga ccgcgcgatc ctcgaggtcg agacccggct tcgcccgcgc
    13321 gacacccggg cgctgctgca ccgcgccgtc gcccggcttg gcgaggtcga aggcgccgcg
    13381 gccatcgttc ccatgctcgc tcgcgccgtc gacaccgcca cccgtcagga catcgacgag
    13441 gtcgcggtcg tggggaccgg ctacgggcgc cagacgctgc cgtttccgaa atcgtgcgtg
    13501 cgcagcgaga ttctgtgcca cgggcgcggc gcgcaccggg tgttcccgcg cacacgcacc
    13561 gtgctcgaca tcggcggtca ggataccaag gcgattcagg tcgacgatcg gggcgtcgtc
    13621 acctcgttcc agatgaacga ccgctgcgcc gccggctgcg ggcgttatct cggctacatc
    13681 gccgacgagc tcaacctcgg gctccacgag ctgggaccgc tggccctcac gtcgaagaag
    13741 atcatcagga tcaactcgac atgcacggta tttgcgaccg ccgagctgcg cgagcgactc
    13801 ggcctcgggc agcgtcgcga ggacatcctc gccggcctgc atcacgcgat catcctgcgg
    13861 gcgatgtcct tgctcgcgcg cagcggcggc gtgaagaacg agttcacgtt caccggcggc
    13921 gtctgcaaca acgcgatggc gaccaaggtc ctgcgcgacc tggtgaacgc gaactacggc
    13981 gaggacatcg tcctcaacgt ccaccccgac agcatctaca tgggcgggct gggcgccgcg
    14041 atgttcgccc tcgaagattt tcgcaccgga caggtggacc tcctgcccag cttcgtcacc
    14101 gacgcccgct cggagatccg atcatgagca ctcgccagca cctgtcggcc ggcatcgacg
    14161 ccggctcgag cgccgtcaag gtcgcgatcg tcaggagcca cgcgggcgag gcacccgagc
    14221 tcctcgccac gactgtccag cgcattcgcc gccgcgacgt gcgcgatgtc gttacggcag
    14281 cgttcgccga ggcctgcgca accgcgaagg tggaggccgc cgtcttcgac tacgtcgcca
    14341 gcacggggga cggcgacgcc gtcggcttcg cgaccgggca cttctacggc atgacgacac
    14401 acgcgcgagg agcgctgtac ctcgtcccca cggcgcgcgc cgcgctggac attggagctc
    14461 tgcacgcgcg cgcgattcgc atggactctc gcggcaaggt cctgggccat cgcatgacca
    14521 gtcagtgcgc cagcgggacc ggtcagttcc tggagaacat cgctcggtac ctgggcgtgc
    14581 cgattgagga cgtcggccgg atgtcgcgcg agtcggcgca ggccgaggag gtctccagcg
    14641 tgtgcgcggt tctcgcagag accgacgtca tcaacatggt gagccgcggg atccgcaccg
    14701 gcgagatcct gcgcgggatc cacctgagta tcgccgggcg cctcgtgcgc ctcctgcgct
    14761 cggccggcgc ggacggcgtc gttgcgctga ccggagggct cgcgctcgac gatgggctcg
    14821 tggcggtcct cgcggagctg ctggcgagcg aaccatcgaa gaacaagaaa cccgtgacac
    14881 cgctgaccgt ggtggcacac cccatggcgg cgctcgctgg cgccatcggt gcggcgatcc
    14941 tcggcgcgta tcgcttcgac cagctggagc agcgaggtcg cacggtgggc gcgtcggcgt
    15001 cggggccagc ctagtgcgga ccgggagcgt agcgggcgat
//
LOCUS       DASVOR010002246         5161 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_492142,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002246 DASVOR010000000
VERSION     DASVOR010002246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5161
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492142"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1104)
                     /locus_tag="VF516_41555"
     CDS             complement(280..1104)
                     /locus_tag="VF516_41555"
                     /inference="COORDINATES: protein motif:HMM:NF014825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HEX8114310.1"
                     /translation="MPATVVDAVQTPLPCACRCHEALSLDDRAAGLAALYRFDDAMRG
                     FGQTVIWDLAAPTLWRLQQQMGGAKWVAVRESGCIHSRLLGFCIHELIHAMCGDPEAP
                     NWGTPVGLPYGVPESVAPVDEAAYLHPFNQNEARAWVGIAAVGYRLFGIEWPLLPARD
                     VGTYGFAGGNALVDVPPGYRAVPHYDHGHHPRRYDALARALEDEARGWFTDDKLDEIA
                     ARFEAAEAMGAARRPRPFPPAREVARLRPKKPGRNDLCMCGSMRKWKQCCGAIMAE"
     gene            complement(1144..1293)
                     /locus_tag="VF516_41560"
     CDS             complement(1144..1293)
                     /locus_tag="VF516_41560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114311.1"
                     /translation="MAAIYFAGGDTGKHTAAHRAPTQRDLVAISTPCQAAGTPTIDGG
                     AATVM"
     gene            1292..2137
                     /locus_tag="VF516_41565"
     CDS             1292..2137
                     /locus_tag="VF516_41565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEX8114312.1"
                     /translation="MHTFRIVNVFTIDGDRFSGNPLCVFDDARGISDAQMQALALQMN
                     LSETTFVLPTDQAGADARVRIFTPGFEMPFAGHPTLGTASVVAGDKPRAVLEMGVGLV
                     PVTRAGNTWTLRTARAPEARPASGTRAEIARMLGLPDGAVCDEPLWVSTGIEQLVIPI
                     ASAALVRTANPVPELLERWGTSPARAQGAMAYVWASDEPTWIVRFFFVANGAVREDPA
                     TGSACANLGGWHVARGRAPMSTTLRQGDAVGRPSRLDLRVDADGGIFVTGGVIELARG
                     SVDLG"
     gene            2264..4474
                     /locus_tag="VF516_41570"
     CDS             2264..4474
                     /locus_tag="VF516_41570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8114313.1"
                     /translation="MTTVAATVHGKVGKGSTTEGGARDHAAALPAVGKRTLTEAILTP
                     SLGSAGVQRKGEAEAPLEGGAASKIARQGTEGPGGPLPHRDRIQSLFGRHDITGVTAH
                     QGPAASQAAQSLGARAYAHGNAVAFASPPDLHTAAHEAAHVVQQRRGVQLKGGIDQPG
                     DGYERHADAVADAVVAGRSAEPLLDQLAAGGQHEATIQRSPAITGASGGPAVTPPAAG
                     IDKTGFIDRKDGANLHTDPIGSADALVRDKPLPPATRVFVSGTHPRGTDWWYVTAFLP
                     DSMVRGYVQGFRINTRLPEPLAELHLVADGETAEGLAKQKFSGVVSDGHDLRYYENVL
                     LYTNQDRHPPGVRGTYQDPGLLGGGNNNVQLVAGELIWLVSAPYARSLQSIVPSGSLT
                     GDAVAKARRFVGHIEDILRSVTESRHHFGEVAGEYAQAIRDHLPEIAGIVAAFIMAEA
                     TSAFLAATPTGVGQIAAVVIQLALAAFGAAGLVQAGIEAVKHASAWLTIAWTASGKDD
                     RIAAASIEFLRMLVSVAIAALSYVGVKGNFGNAVKIASSMPAGALPAFAVAGGGQMTS
                     GAGAGTGVLIGPSTGSLGTAGNAMMQADNNSGGGGDEGARSQDPARELEEIKQKLESD
                     ELSGKQKQALRARKKQLQEQLGKTSAEPTPEDIPTPTEFKTRASGLSGKEAATDVPSW
                     MDNWPDARPGVNENGTTFATRMMNKRYGVGRWMMKGDQYEEFNKLRKFADRAFK"
     gene            4480..5019
                     /locus_tag="VF516_41575"
     CDS             4480..5019
                     /locus_tag="VF516_41575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114314.1"
                     /translation="MSSENPSNTPTASIEPLWRLVAIGLDPDKTVPDLYGAIHQGEPD
                     VPLMVDGRIVFFTDPGRAPELIQKYGGAWANDPTDVSKPTLWCDIAQALHHLSAGGID
                     TSTSIVDAVNVLLDLVKATGVKIVDGRRKALYAIADYCTFNKDLTKYLEEEGDYSSRE
                     LVDAVLWCVGAIVVKSRIL"
BASE COUNT          834 a         1832 c         1760 g          735 t
ORIGIN      
        1 ctcggcctcg gccctggcct ccggggccgg ggccggcacg gtgaccgccg gcgccgtcga
       61 gccgcagccg acgccggcgg tgccacatcc caggaacacc gcgagtccga gtgagcagcg
      121 cttcatggca gcgcatccta ctcctggact tgcgtggttg cggatccgga gaggaccgag
      181 gcagcgcgca acactacttc acatgccagc gcggtgggcg gtcgcgcgcg atcgaggctc
      241 cggcgcctca tgcgcaatcg cgtcattcac tcggctcgtt cactcggcca tgatcgcgcc
      301 gcagcactgt ttccacttcc gcatcgaccc gcacatgcac aggtcgttgc ggcccggctt
      361 cttcggccgg agccgcgcga cctcgcgagc agggggaaac ggccgcggcc gccgcgccgc
      421 gcccatggcc tcggcggcct cgaaccgcgc ggcgatctcg tcgagcttgt cgtcggtgaa
      481 ccagccgcgc gcctcgtcct cgagcgcccg ggcgagcgcg tcgtagcggc ggggatggtg
      541 gccgtgatcg tagtgcggca cggcgcggta gcccggcggc acgtcgacca gcgcgttgcc
      601 gccggcgaag ccgtaggtcc cgacgtcgcg cgccgggagc agcggccact cgatcccgaa
      661 cagccggtag ccgaccgcgg cgatcccgac ccaggcgcgg gcctcgttct ggttgaacgg
      721 gtggaggtac gcggcctcgt cgaccggcgc caccgactcc ggcacgccgt acggcaggcc
      781 gaccggcgtc ccccagttcg gcgcctcggg atcgccgcac atcgcgtgga tcagctcgtg
      841 gatgcagaac ccgagcagcc ggctgtggat gcagccgctc tcgcgcaccg cgacccactt
      901 cgcgccgccc atctgctgct gcagccgcca cagcgtcggc gcggcgaggt cccagatcac
      961 cgtctggccg aacccgcgca tcgcgtcatc gaaccggtac agcgcggcga ggccggcggc
     1021 gcggtcgtcg agcgacagcg cctcgtggca ccggcacgca cagggcagcg gggtctggac
     1081 ggcatcgacg acggtggccg gcacccggct atcttacccg cggcccggcc gggcgcccgg
     1141 gatctacatc acggttgctg cgccgccgtc gatcgtcggg gtgccggccg cctggcacgg
     1201 cgtcgagatg gccacgaggt cgcgctgtgt cggcgcgcgg tgcgccgcgg tgtgcttgcc
     1261 ggtgtcgccc cccgcgaagt agatcgctgc catgcacacg ttccgcatcg tcaatgtgtt
     1321 cacgatcgac ggcgatcggt tctcggggaa tccgctgtgc gtgttcgacg acgcgcgcgg
     1381 gatcagcgat gcgcagatgc aggcgctcgc gctccagatg aacctgtcgg agaccacgtt
     1441 cgtgctgccg accgaccagg ccggcgccga tgcccgggtc cggatcttca cgcccggctt
     1501 cgagatgccg ttcgcggggc atccgacgct gggcaccgcg agcgtggtcg ccggcgacaa
     1561 gccgcgcgcc gtgctcgaga tgggcgtggg cctggtgccg gtgacgcgcg ccggaaacac
     1621 ctggacgctg cgcaccgcgc gagcgccgga ggcccggccg gcgtcgggca cacgcgccga
     1681 gatcgcgcgc atgctcgggc tccccgacgg cgcggtgtgc gacgagccgc tgtgggtgtc
     1741 gaccggcatc gagcagctgg tgatcccgat cgcgtcggcc gcgctggtcc gcaccgccaa
     1801 tccggtgccc gagctgctcg agcgctgggg cacctcgccg gcgcgggccc agggcgcgat
     1861 ggcctatgtg tgggcctccg acgagccgac ctggatcgtg cggttcttct tcgtcgccaa
     1921 cggcgcggtg cgcgaagatc cggcgaccgg ctcggcgtgc gccaacctcg gcggctggca
     1981 cgtcgcgcga ggccgcgcgc cgatgtcgac gacgctgcgc cagggcgacg cggtcggccg
     2041 gccgtcgcgg ctcgacctcc gggtcgacgc cgacggcggc atcttcgtga ccggcggcgt
     2101 gatcgagctc gcccgcggca gcgtcgacct cgggtagcca acccgacctg ggacccgcgc
     2161 accgctggac tcgcaggacg gcctcacggc ggcgtggtca ggcctcgcgt tgccacgggt
     2221 tgccctgcca tccgtcgcgc tggcgcttga gcgacggtct gcgttgacta ccgtcgcagc
     2281 caccgtgcac ggcaaggtgg gcaagggcag cacgacggag ggtggcgcac gtgatcatgc
     2341 cgcggcgtta ccggccgtgg gcaagcggac cctgaccgaa gcgatcctca ccccgagtct
     2401 cggcagcgcc ggggtgcagc gcaagggcga ggcagaagcg ccgcttgaag gtggcgctgc
     2461 gtcgaagatc gctcgccagg gcacggaggg tcctggcggt ccactaccgc accgcgaccg
     2521 gatccagtcg ctgttcgggc gtcacgacat caccggcgtg acagcgcacc agggccctgc
     2581 ggcaagccag gcagcgcaat cgctcggcgc ccgagcgtac gcccacggaa acgccgtcgc
     2641 attcgcttcg ccgccggacc tccacaccgc ggctcacgag gccgcccacg tcgtgcagca
     2701 acgcaggggc gtgcagctca aaggcggcat cgaccagccg ggggacggct acgagcgcca
     2761 tgccgacgcc gtcgccgatg ccgtcgtcgc cggcaggtcc gccgagccgc tgctcgacca
     2821 gctcgccgcc ggcgggcagc acgaggcgac tatccagcgc tcgcccgcca tcaccggtgc
     2881 cagtggcggc ccggccgtca caccgccagc ggccggcatc gacaagaccg gcttcatcga
     2941 tcgcaaggac ggcgccaacc tgcacaccga ccccatcggg tcagccgacg cgctcgtccg
     3001 cgacaagccg ttaccgccag caacacgcgt gttcgtcagc ggcacgcatc cacgaggtac
     3061 cgactggtgg tacgtcaccg cgttcctccc cgactcgatg gtgcgaggct acgtccaggg
     3121 cttccgcatc aacacgcgcc tccccgagcc gctggccgag ctccacctcg tcgccgacgg
     3181 cgagaccgcc gaagggctcg cgaagcagaa gttctccggc gtcgtctccg atggccacga
     3241 cctccgctac tacgagaacg tcctgctgta caccaaccag gatcgccacc cgccgggtgt
     3301 ccgaggcacc taccaggatc ccggcctgct cggcgggggc aacaacaacg tccagctcgt
     3361 ggccggcgag ctgatctggc tggtcagcgc cccctacgcc cgatcactcc agagcatcgt
     3421 gcccagcggc tcgctcaccg gcgacgcggt cgccaaggcc aggcgcttcg tcggccacat
     3481 cgaggacatc ctccgcagcg tcaccgagtc gcgccatcac ttcggcgagg tcgccggcga
     3541 atacgcccag gcgatccgcg atcaccttcc cgagatcgcc ggcatcgtcg ccgcgttcat
     3601 catggccgag gcgacctcgg ccttcctcgc cgccaccccg accggcgtcg gccagatcgc
     3661 cgcggtcgtg atccagctcg ccctcgcggc gttcggcgcc gccgggttgg tccaggccgg
     3721 catcgaggcc gtgaagcacg ccagcgcgtg gctgacgatc gcgtggaccg cgagcggcaa
     3781 ggacgaccgg atcgccgctg ccagcatcga gttcctcagg atgctcgtca gcgttgcgat
     3841 cgcggcgcta tcgtacgtcg gcgtcaaggg caacttcggc aacgcggtca agatcgccag
     3901 ctcgatgccg gccggcgccc tgccggcgtt cgcggtcgcc ggcggtggtc agatgaccag
     3961 cggcgccggt gctggaaccg gcgtcctgat cggcccgagc accggctcgc tgggcaccgc
     4021 cggcaacgcc atgatgcagg ccgacaacaa cagcggcggc ggcggcgatg agggggcaag
     4081 gtcgcaggat cccgccaggg agctcgagga gatcaagcag aagctcgagt ctgatgagct
     4141 cagcggtaag cagaagcagg cgcttcgcgc acggaagaag cagcttcaag agcagctggg
     4201 caagacgtcc gccgagccaa cgcccgaaga catccccacg cctaccgagt tcaagacgcg
     4261 cgcttcgggg ctgagtggca aggaagcggc cacggacgtc cccagctgga tggacaactg
     4321 gcccgatgcc aggcccggcg tgaacgaaaa tggtactacg tttgctactc gtatgatgaa
     4381 taagcggtat ggtgttgggc gatggatgat gaaaggtgat caatacgaag agttcaacaa
     4441 gctcaggaag ttcgccgatc gcgccttcaa gtagaagcca tgtcatccga gaatccatca
     4501 aacacgccta ctgcctcgat cgaaccactc tggcgtctgg tcgccatcgg actcgatccg
     4561 gacaagaccg tgcccgacct ctacggtgcg atccatcaag gcgagcccga tgtaccgctc
     4621 atggtcgacg gacgcatcgt gttcttcacg gatcctgggc gcgcacccga gctgatccaa
     4681 aagtacggcg gcgcatgggc aaacgatccg acagacgtga gcaagccgac gctgtggtgc
     4741 gacatcgcgc aagcactgca ccatctgtcc gccggcggta tcgacacgtc gacctcgatc
     4801 gtggatgcag tcaatgtgct cctggatctg gtgaaggcga cgggcgtcaa gatagtcgac
     4861 ggccgcagga aggcgctgta tgccattgcc gattactgca ccttcaacaa agatctgacg
     4921 aagtacctcg aagaagaagg tgattactcc agtcgtgagc tggtcgacgc ggtactctgg
     4981 tgcgtcggcg ccatcgtcgt caagtcacgg atcttgtagc cggatacgct gacctgtcac
     5041 cgctggacca gcctccaagg gcgctccatc ggcctcgagc gacacaagga tcgacgccgt
     5101 cgactgcaaa attgcctggc gcgggccggc gtgatcgcgc atgtcccagg gatgtctgca
     5161 c
//
LOCUS       DASVOR010002247         3591 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_403342,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002247 DASVOR010000000
VERSION     DASVOR010002247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403342"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1090..1899
                     /locus_tag="VF516_41580"
     CDS             1090..1899
                     /locus_tag="VF516_41580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114315.1"
                     /translation="MGSILQPQLGWSHIVIMAAGLLVVYEICYRIARWRREETRDAKK
                     SQADVAIAALLALLGLLLAFSFQIGAERFDKRKALVLDESNAITTTYLRATLVQAPYD
                     QRIQELLREYVRVRANVTSADELERALKESGRLHGELWANATAIAHRSPDSPVSALFI
                     ASLNNMIDLHEARVTVGLYQRLPPAIFASLYFVALLSVGMVGIRAGLDRVRSLIPVTL
                     LVASVMCVIGLIASLDQPMSRLFSISKHAIEDTQRILTNSSPGTTATAEPK"
     gene            2421..3443
                     /locus_tag="VF516_41585"
     CDS             2421..3443
                     /locus_tag="VF516_41585"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEX8114316.1"
                     /translation="MGAANTVALSAGHAQGSSMTLKCAMNDYVVMLSRLVPRMRPFLA
                     ALVAVAITTQGRGASAQALEPRSYVNTPVGINFVLVGYGYRRGGVGLDESSTVQDIKV
                     GVHAGLLSYARSLDVWGLSGKIVGVLPVAEASGTATVVGQRQTRHVFGLGDPLLRVSV
                     NVYGASALSVKQFPSYRQDVIVGVGLGVSAPLGQYDSTKLLNVGTNRWAVRPELGVSK
                     ACDRFTLELISAIMLFTTNDDLLGGKTLDQAPIYSLQGHLIYESSSVFWAALDTTYYA
                     GGRTTVDGEPGPAPGNARLGLTTALSLSSYQSIKLTGSIGVYHRTANDFRAVGIAWQY
                     RWGEGL"
BASE COUNT          636 a         1141 c         1192 g          622 t
ORIGIN      
        1 agtggggtag caagatcggg accctcagat cgatgcttgg cgttgacgac ggtggggacg
       61 tgcccctact tctacgccaa agccgtacga cgaccgctcc gcgatcttcg aggcagcctt
      121 ccacaagcgc ccggtgtcct cggaagggcc ggcgctcgag ctggtgaccc tcttgcaact
      181 caccgtgaac cagaggtcag agcggcagcc cgccaggatc gtggtggcaa cgcgcttaag
      241 cgcagcttcg accgtcagcg agctggcgaa cacggggacc gcggggagca agcccgccag
      301 ccagaggctg aacaggttcc gcggcccgaa cacgaatagc cgagtcttca cgatcacctg
      361 gaaactagtt gccgatgtcg gcgagcgact ggatatcgag cgagcccatc acctcgtcga
      421 tctcgagcgg gcgggcgatc atgaacgacc tcgcgctcgc cgaccagtac gccatgctgc
      481 atcgcacgat tgagccgcag tcgtgtatac aagacaccga agacgccgac agcagagctt
      541 ttcgtgtgta ggacgcatgg ttcacatgat ctctctgtgc gctgtgaagg aagatcgagc
      601 ctggatccct gcggacggcg cctctccgcc tcgcgacgaa ctcgaccgcg actgggcgca
      661 gcgaggacct cggcgatcaa cctggtcggc cgggtgttcg gccgggcggg cagcgagcgg
      721 taggatgagc tcgtgattga cgagcgcagg tgtggaacac gcgcgccgct gacgcacggc
      781 cggcaccatc ggcggaccgc gtagtcacgc gacgaccgcg aactccgcac gaccgggaac
      841 aattgctgca tcgaattagg caaggagtaa ctcatgggca tatcggcaag cgcggcagca
      901 cgcaccaacg ttcgttctcg caggccccag ccgcagcctg cgaccgagaa caatacgctg
      961 atcggaaact tcccaggttc agtcgcctca cgcacgctag tcaacgccgc gtacacgctg
     1021 gcaggctgcc agcttccgat ctagtctagc tgcggcatct gctgcagatc gcatgatgtg
     1081 gagggctcga tgggctcgat tctgcaacca caactcggct ggtcgcacat cgtgatcatg
     1141 gcggcaggtc tgctcgtcgt ttacgagatc tgttaccgga tcgcgcggtg gcggcgcgag
     1201 gaaacgcgcg acgcaaagaa gtcacaggct gacgtcgcga tcgcggcact cctcgcgcta
     1261 ctcggactgc tcctcgcgtt cagcttccag atcggagccg agcggttcga caagcggaag
     1321 gcgctcgtgc tcgacgagtc caacgcgatc acgacgacgt atctgcgggc gaccctggtg
     1381 caagcgccgt atgatcagag gatccaggag ttgcttcgcg agtacgtccg ggtgcgcgcc
     1441 aacgtgacgt ccgcagacga gctggaacgc gcgctcaaag agtcggggag gctacatggt
     1501 gagctgtggg ccaacgcgac ggcgatcgcg catagaagtc ctgactcgcc agtctccgcg
     1561 ttgttcatcg cctcgctgaa caacatgatc gacctccacg aggcccgcgt caccgtgggg
     1621 ctgtaccagc ggctcccgcc ggcgatcttc gcgagcctgt actttgttgc gttgctctcg
     1681 gtcggaatgg tcgggattcg agccggtctc gatcgcgtgc gcagtctgat ccccgtcacg
     1741 ctcctggtcg cttcggtcat gtgcgtgatt gggctcatcg cctctctcga ccagccgatg
     1801 agtcggctgt tcagcatcag caagcacgcg atcgaggaca cgcaacgaat cctaacaaac
     1861 tcctcgccag gcacgacggc gacggcagaa ccgaagtagg ccggggcaca gcgagcgcgc
     1921 gccgcgcctc gcttacggtc gcgggcggtc gacatcgccg gcgccgccct ggttcagggc
     1981 gcagcgcggt cgacggcggt cgacgtcgcc ctggtcgagg aggaggacga gctgagcagc
     2041 gagctcgggg cgcgccgccc gatggacacc ggcgttgagc acacgatgct cggcgtcggc
     2101 gctgtgggca aatttgtcgg cgtcccgctc gcgaatggat ggacagtcag ctcgcgcttt
     2161 gcttcgggag agcggtggtt gcgatggcct gttccggtct cccgaccgtg ctttcgaggt
     2221 cacggctact gcggcgaggt gtgccgcgag cgcgctcgcg ccgcggctgg cgcgggctcg
     2281 tcaccagcag tcgccgcacg gtctcgctga ccatcggcaa aatcggctga gcgcgctcca
     2341 gagcctgaat ctgcgatttc tcttcgctac acagcacggc agcgttgtca ggcgggctgt
     2401 gcggcgacgc tcggctctcg atgggcgcgg cgaacaccgt tgccctttct gcgggccacg
     2461 cgcaaggttc gagcatgacc ctcaagtgcg ccatgaacga ctatgtcgtg atgctgtcgc
     2521 gtctcgtccc acggatgcgc ccgttcttgg ccgcgctcgt ggcggtggcg atcacgacgc
     2581 aaggacgcgg cgcgtccgcg caggcgctag agccgaggtc ctacgtgaac accccagtcg
     2641 ggatcaactt cgtgctggtc ggctacggct acaggcgagg tggcgtcggc ctcgacgagt
     2701 cgagcacagt gcaagacatc aaggtcgggg tccacgccgg cctgctgtcc tacgcccgat
     2761 cgttggatgt gtgggggttg tccggcaaga tcgtaggcgt gctgccggtc gccgaggcat
     2821 cgggcaccgc gacggtcgtg ggccagagac agactcgtca cgtgttcggg ctcggcgacc
     2881 cgctgctccg cgtctctgtc aatgtctatg gcgcgtcggc tctatcggtg aagcagttcc
     2941 cgagctaccg gcaggacgtg atcgtcggcg tcggcttggg ggtgagcgct ccgctcggcc
     3001 agtacgactc gaccaagctg ctgaacgtcg ggacgaatcg ctgggccgtg aggccggagc
     3061 tcggcgtatc gaaagcgtgc gaccgcttca cgctggagct gatctcggcg atcatgttgt
     3121 tcaccacgaa cgacgactta ttgggtggga agacactcga ccaggccccc atctactcgt
     3181 tgcagggaca cctcatctac gagtcctctt cggtgttctg ggccgcgctc gataccacct
     3241 actacgccgg cggacgcacg accgtcgacg gcgagccggg accggcgccg ggaaacgccc
     3301 ggttgggctt gaccacggcg ctgtccttga gcagttacca gtcgatcaag ctcacgggta
     3361 gcatcggcgt ataccaccgc accgccaacg acttcagggc ggtcgggatc gcctggcagt
     3421 atcggtgggg tgaagggttg tgagcacgag cgtaccaact cgcctgcgta ctcgtcgtgg
     3481 ccgagcctcc aaccagcgcg tgatgaagcg atacggcaag agccgacgcg cgctgttcga
     3541 cgcgctcgat agcagccccg tgctggctac caatctcgtc cggcaggatc g
//
LOCUS       DASVOR010002248         2137 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_228958,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002248 DASVOR010000000
VERSION     DASVOR010002248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2137)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2137)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2137
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_228958"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="VF516_41590"
     CDS             <1..297
                     /locus_tag="VF516_41590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114317.1"
                     /translation="ELDVGGERAIRLALAQDLGGRAPLGDLRLDHNTLTTGLVTVRSG
                     RCDRAMVRSIRDQIAELRVALTPREQDAVATVIAAIIWAVLDASARALDATLVA"
     gene            complement(679..1326)
                     /locus_tag="VF516_41595"
     CDS             complement(679..1326)
                     /locus_tag="VF516_41595"
                     /inference="COORDINATES: protein motif:HMM:NF013045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEX8114318.1"
                     /translation="MIVHRDARCVVVDKPSGLATHRGWDGGDDALLQRVRDAVGCYVY
                     PVHRLARGASGLVVFALDGDAARALAAAWPDADKRYLAITRGHPADHAVIDHPIPRAP
                     GEDRVAARTEVWRRETFGRYALVEARLHTGRLHQIRRHLKHISCPLIGDVRYGKGEHN
                     RIFRTEHGLSRLALHCTRIAVGHPDGGELAVTSALPDDLAGAIASCRRRYAENAG"
     gene            complement(1370..1615)
                     /locus_tag="VF516_41600"
     CDS             complement(1370..1615)
                     /locus_tag="VF516_41600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module protein"
                     /protein_id="HEX8114319.1"
                     /translation="MAIEEVLRDALALSDEDREKLLVALHQSLEPDDGDTLSQPEWDA
                     AWAAEIDRRLKDLAEGRARTYTHQEVMAELRARLHRR"
     gene            complement(1630..>2137)
                     /locus_tag="VF516_41605"
     CDS             complement(1630..>2137)
                     /locus_tag="VF516_41605"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEX8114320.1"
                     /translation="RDLITVPLDDQIAALQAIRVRHPELDLSRVGVFGWSFGGYFSTM
                     AALLRPDVFKAAVAGAPVTDWTLYDTAYTERYMKTPAANPDGYRATSALTHAARLTRP
                     LLVLHGITDDNVHFAHTLALIEALYVAGKRAEVITLSATHMLPDPKLAVARERVQVDF
                     FRQHLGPP"
BASE COUNT          303 a          720 c          794 g          320 t
ORIGIN      
        1 gaactcgacg ttggcggcga gcgagcgatc cggcttgcgc tcgcgcagga tcttggcggc
       61 cgcgcgccgc tcggcgacct ccggctcgat cacaacacgc tcaccaccgg cttggtgacg
      121 gtgcgcagcg gccgctgcga tcgcgcgatg gtgcggtcga tccgcgatca gatcgccgag
      181 ctgcgcgtcg cgctgacccc gcgcgagcag gacgcggtcg ccaccgtgat cgccgcgatc
      241 atctgggccg tgctcgacgc cagcgcgcgg gcgctcgacg ccacgctggt cgcctagcac
      301 ccaggagaac gcggacacgc tcgctcgccg gggctgctct gatgattcgt cacaagccgt
      361 cgatcaggcg ttcgtgatcg tcgacgtcga cctgatcgtt gacccagagc gttgagctcg
      421 acggcgactt acgacgttga gcccaccatc gaggttgaac gtcgaccgat ccctgtgtcg
      481 cgagcctcga cggagcgcga cgacagggcg aggtcgaggt caaaggtggg gtcagacgtc
      541 tacgtatcgc ttcgctgccg tcgagctcaa cgctcgagtc aaggtcgacg tcatcgtcga
      601 cgttcgtcgg tatggacgtc gagcgacacg ggagcggcta cgagcgcgca acttgtgacg
      661 aatcatcaga ccccctcgct agcccgcgtt ctccgcgtag cgccgccggc agctcgcgat
      721 cgccccggcg agatcgtcgg gcagcgcgga cgtcaccgcg agctcgccgc cgtcggggtg
      781 gccgaccgcg atccgcgtgc aatgcagcgc gaggcgggac aggccgtgct cggtgcggaa
      841 gatccggttg tgctcgccct tgccgtagcg cacgtcgccg atcagcgggc agctgatgtg
      901 tttgaggtgg cgccggatct ggtgcagccg gccggtgtgc agccgggcct cgaccagcgc
      961 gtagcggccg aacgtctcgc gccgccacac ctcggtgcgc gccgcgaccc ggtcctcgcc
     1021 cggcgcgcgc ggaattggat ggtcgatcac cgcgtggtcc gccgggtggc cgcgcgtgat
     1081 cgccaggtag cgcttgtcgg cgtcgggcca cgccgccgcg agcgcccggg cggcgtcgcc
     1141 gtcgagcgcg aacaccacca gccccgacgc gccgcgagcg agccggtgca ccgggtagac
     1201 gtagcagccg accgcgtcgc gcacccgctg cagcagcgcg tcgtcgccgc cgtcccagcc
     1261 gcggtgggtc gccagccccg acggcttgtc gaccaccacg cagcgcgcgt cgcgatggac
     1321 gatcatcgga gccggtcgag ccagtaaccg ggccggcggc tctcgcgcat cagcggcggt
     1381 gcaggcgagc gcgcagctcg gccatgactt cctgatgcgt gtacgtccgg gctcgacctt
     1441 cggcgagatc tttcagtcgg cggtcgatct cggcggccca ggcggcatcc cactcgggtt
     1501 ggctcagggt gtcgccgtcg tcgggctcca ggctctggtg gagcgcgacg agaagcttct
     1561 ctcgatcctc atcgctgagc gcgagcgcgt ctcgcagaac ctcttcgatc gccacgcgag
     1621 gagtgtagct cacggcgggc cgaggtgctg gcggaagaag tcgacctgga cgcgctcgcg
     1681 cgcgacggcg agcttcggat ccggcagcat gtgggtcgcc gagagggtga tgacctcggc
     1741 gcgcttgccg gcgacgtaga gcgcctcgat cagcgcgagg gtgtgggcga agtggacgtt
     1801 gtcgtcggtg atgccgtgga ggaccaggag cggccgggtc agccgggcgg cgtgggtcag
     1861 cgcgctggtc gcgcggtagc cgtcggggtt ggccgcgggc gtcttcatgt agcgctcggt
     1921 gtacgcggtg tcgtagagcg tccagtcggt gaccggcgcg ccggcgaccg cggccttgaa
     1981 cacgtcgggg cgcagcagcg cggccatggt cgagaagtag ccgccgaacg accagccgaa
     2041 cacgccgacc cgcgagaggt cgagctcggg gtggcggacc cggatcgcct ggagcgcggc
     2101 gatctggtca tcgagcggga cggtgatcag gtcgcgc
//
LOCUS       DASVOR010002249         1999 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_375071,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002249 DASVOR010000000
VERSION     DASVOR010002249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1999
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375071"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..975)
                     /locus_tag="VF516_41610"
     CDS             complement(43..975)
                     /locus_tag="VF516_41610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114321.1"
                     /translation="MAEGLIGPGFYPQPNEWTCGPFALKHALIALGRMVDVNQISTTA
                     RTHWWSGTNEIQLARAARAFECDLVLERRADAEQARKVLVKYLREQTPVLLCVDEWSH
                     WITVLRAEDRRFVVVDSTDDPLLSVRTWPQLRNWWRYHDTDYVKDNPPVLYDLMAVAP
                     RFRTTVKADFSVERVKFLRRPENRRLAHHWNEYLEDLLEICKPPSVRIAEPLSMGEFL
                     RRHQELLLTRVVYWHGDIHRDEVGRVLRDMRFVSETYGLVIPASMSRRALADLAILIS
                     IWACSQRGVDGMFGSPGADARPEPKSSRKRNDRK"
     gene            complement(1050..1430)
                     /locus_tag="VF516_41615"
     CDS             complement(1050..1430)
                     /locus_tag="VF516_41615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114322.1"
                     /translation="MVVFSNRRLGPGAELLVSREVAGELLREVAAAAGPAAQARWEHE
                     LVSWLERCAASGSTSIDVADIAWTPEHFERQRRFLVEAIQRAQASSRHGRALTRWCGM
                     IEAHPRESVLVGRRWQWPTADLTV"
     gene            complement(1708..>1999)
                     /locus_tag="VF516_41620"
     CDS             complement(1708..>1999)
                     /locus_tag="VF516_41620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114323.1"
                     /translation="DGGLQRGQRDPVGAGLGEPTGLLVGPDHALVEQGAIGVEVDQAG
                     QGAQRELAIAGSTGPSAGRSARRSTSSTAGPACRPSRAPGRRFRDRRSSPIS"
BASE COUNT          307 a          739 c          670 g          283 t
ORIGIN      
        1 atcactgacc cggtgcgccc cggtgcgccc cggcgcggac cgctacttgc ggtcgttgcg
       61 cttgcgcgac gacttgggct cgggtcgcgc gtcggcgccc ggcgagccga acatgccgtc
      121 gacgccgcgc tgcgagcacg cccagatcga gatcaggatc gcgagatcgg cgagcgcgcg
      181 ccggctcatc gacgccggga tcaccaggcc gtaggtctcg gacacgaagc gcatgtcgcg
      241 cagcacgcgg ccgacctcgt cgcggtggat gtcgccgtgc cagtacacga cgcgggtcag
      301 cagcagctcc tggtggcggc gcaggaactc gcccatcgac agcggctcgg cgatccgcac
      361 cgacggcggc ttgcagatct cgagcaggtc ctcgaggtac tcgttccagt ggtgggcgag
      421 ccggcggttc tccggccggc gcaggaactt cacgcgctcg accgagaagt ccgccttgac
      481 ggtggtgcgg aaccgcggcg ccaccgccat caggtcatag agcaccggcg ggttgtcctt
      541 gacgtagtcg gtgtcgtggt agcgccacca gttgcgcagc tgcggccagg tccgcaccga
      601 cagcagcgga tcgtcggtcg agtcgacgac cacgaaccgg cggtcctcgg cgcgcagcac
      661 cgtgatccag tgcgaccact catcgacgca cagcagcacc ggcgtctgct cgcgcaggta
      721 cttcacgagc accttgcggg cctgctcggc atcggcgcgg cgctccagca ccagatcgca
      781 ctcgaacgcg cgcgccgccc gggcgagctg gatctcgttg gtgccggacc accagtgggt
      841 ccgcgcggtc gtcgagatct ggttcacatc gaccatgcgg ccgagcgcga tcagggcgtg
      901 cttgagcgcg aacggaccgc acgtccattc gttcggctgc gggtagaacc ccggtccaat
      961 gagcccctcg gccacgagcc cactctagca ctgccgcatt gcgtcgcgac ttccgtgagc
     1021 gaaagtgaac ggaatttacc tgctgaaaat cagacggtta gatcagcggt cggccattgc
     1081 cagcggcgcc cgaccaacac cgactcccgg gggtgagcct cgatcatgcc gcaccaccgg
     1141 gtgagcgcac ggccgtggcg cgagctcgcc tgggcgcgct ggatcgcctc gaccagaaag
     1201 cggcgctggc gctcgaagtg ctctggcgtc cacgcgatgt cggcgacgtc gatgctggtg
     1261 ctgcccgacg ccgcgcagcg ctcgagccag ctcacgagct cgtgctccca gcgcgcctgc
     1321 gccgccgggc ccgccgcggc cgcgacctcg cgcagcagct cgccggcgac ctcgcgcgac
     1381 accagcagct ccgcgccggg gccgagacga cggttggaga acacgaccat ggccgacagg
     1441 ataggcacgg ccctctgaca gtttcgtgac accggcgtag ccttgctgcg accgtccggc
     1501 ccccggacag acgtcccaca ccccggctgc gacggatcgc ctcaccgtcg acagcatcgc
     1561 gccgtgaccc gaccgccatc ggggaccgcc tcaccgtggg ccggcgtgag cgcccatcgc
     1621 acagcggcta gccacgcctg cgaagatcgc gcgttgaagc cgtcatggtc gaccgccgcg
     1681 cgcatggttt cctgacatct ccaggggtta cgagatcggt gacgatcgcc ggtcacgaaa
     1741 tcgccgcccc ggcgcacgtg acggccgaca tgcaggcccc gctgtgctcg acgtcgatcg
     1801 cctggccgat cgcccggccg atggccccgt cgatccggcg atcgcgagtt cgcgctgcgc
     1861 accctggccg gcctggtcga cctcgacccc gatcgcgccc tgctccacga gcgcatggtc
     1921 cggacctacg agcaggccgg tcggctcgcc cagtcctgcg cccaccggat cgcgctggcc
     1981 gcgctgcagc ccaccatcg
//
LOCUS       DASVOR010002250         3286 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_492187,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002250 DASVOR010000000
VERSION     DASVOR010002250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492187"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VF516_41625"
     CDS             complement(<1..967)
                     /locus_tag="VF516_41625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114324.1"
                     /translation="MARNSPTAPPPASAARAAATVGLATTGDATLVSISGLVDERFMG
                     FGDIGAARTVVLDVSGMTRMTSFGVRQWLKAMDALPKSITDLYLLGCPTFFVDQLNMV
                     LNFGGPAKILTVVAPHTCPSCGVESGELIDVLVDRANLVKGGLPEKECARCGGKLEFD
                     ETPEAYFAFVGKYAASSLGPAAAQLLAAQGLYTSSESASEKPPRIIKLVHDSVTYFRI
                     IGSIGSTFRARPFLVGAEGEVVIDLAEVERFDSAGQKEWRRLVKSLAGQVPAVTFVDV
                     SESFLASASDTLTLARNIAVASVLVPYGCTECDRTSYESVPAAWPM"
     gene            1462..2472
                     /locus_tag="VF516_41630"
     CDS             1462..2472
                     /locus_tag="VF516_41630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114325.1"
                     /translation="MRSIVWTSEPLSRALRDLLPSEVVVASSASEFFAAKRDAKTVSI
                     IDQVVLGDFNRAALESGVRVDQIGPVITIFDEPLQNTIGWVAAHPWLSHVMSSGMLRH
                     PIAAEHLRNVIATLTSNRPPRLLDWLGPTINGRRVRLARASKRVERLERLTAYLESRG
                     VGERTVQQLRDAAEELLTNAFYDAPAAAGVTQPISRTRDVRLPEDSACDMVYGCRDDL
                     AVVRVRDPFGSLSRARLIEVLMRCARTDMQVEVDETMGGAGLGLWRIFTVASFVAIAV
                     VRNRHTEVLVGIGKRVPSARRPFAFHFFFREDARAAPGWRLQDADTTQPQVNQSVTIV
                     TK"
     gene            2500..>3286
                     /locus_tag="VF516_41635"
     CDS             2500..>3286
                     /locus_tag="VF516_41635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114326.1"
                     /translation="MIRHSKPPSAAPPPPARAAATVGLATKGDAVLVSISGLVDERFT
                     GFGNLGAARTVILDVSGMTRMTSFGVRQWLKAMDALPKSITELYLLGCPTFFVDQLNM
                     VLNFGGPAKILTVMAPHTCPSCGVESGALIDVLADRANLVKGGLPEKECARCGGKLEF
                     DETPESYFAFVGKYAASSLGPAAAQLLAAQGLYTSSESASEKPPRIIKLVHGSVTYFR
                     IIGSIGSMFRARPFLVGAEGEVVIDLAEVERFDSAGLNEWRRLV"
BASE COUNT          522 a         1139 c         1103 g          522 t
ORIGIN      
        1 gcatcggcca ggccgccggc acgctctcgt acgacgtccg gtcgcactcg gtgcagccat
       61 acggcaccag caccgaggcc accgcgatgt tgcgggccag ggtgagcgtg tcgctcgcgc
      121 tggccaggaa cgactcgctg acgtcgacga acgtcaccgc cggcacctgc ccggccaggc
      181 tcttgaccag gcgccgccac tccttctgcc ccgccgagtc gaaccgctcg acctctgcga
      241 ggtcgatgac gacctcgccc tcggcgccga ccaggaacgg ccgggcccgg aacgtcgacc
      301 cgatcgaccc gatgatccgg aagtacgtca ccgagtcgtg gaccagcttg atgatccgcg
      361 gcggcttctc gctggcgctc tccgacgagg tgtacagacc ctgcgcggcc aggagctgcg
      421 cggccgccgg cccgaggctc gacgccgcgt acttgcccac gaacgcgaag taggcctcgg
      481 gcgtctcgtc aaactcgagc ttgccgccgc accgcgcgca ctccttctcg ggcaggccgc
      541 ccttgaccag gttggcgcgg tcgacgagca cgtcgatcag ctcgcccgac tcgacaccgc
      601 acgacgggca ggtgtggggc gccacgaccg tcaagatctt cgccgggccc ccgaagttca
      661 gcaccatgtt gagctggtcg acgaagaacg tcggacagcc cagcagatag agatcggtga
      721 tcgacttggg cagcgcgtcc atcgccttga gccactgccg cacgccgaac gaggtcatcc
      781 gggtcatccc ggacacatcg aggaccacgg tccgggcggc cccgatgtcg ccaaatccca
      841 tgaaccgctc gtcgaccagg cccgagatgc tcaccagcgt cgcatcgccc gtggtcgcca
      901 gaccgacggt ggccgccgcg cgcgccgccg acgccggggg aggggccgtg ggtgagttcc
      961 gggccatgat gttcccacgc tacgcgagga agcccgcgcc cgcgcccgcg ccaatcccgg
     1021 aatggctagc gccggcgatc gtccagagaa ggagatccgc cattcgcagt gcaagctcgt
     1081 cggatgatct gaccctgcag atcggcgtca ccggcgcagt gtaacaccat gtggtgggcg
     1141 tcgcgacccc ccacccgcgc tgggcgcaga tccggaacga cggcccgaag gccgaaacct
     1201 gacgattccg catacttggt cccgagcctc gaatccccat cactgtgagt cgccgtcatt
     1261 cgcagcccga ccccgcggtg cgtgatcaaa cgcgcagtcc ggctcgtccc cgggcagcgg
     1321 ttgcgcgctc ctctgccccc ggtcgatgag caccggatca ctcctcggag cgaaacgctg
     1381 cgcgccgaaa cgaccgatcc tctggcggag gatcgcgaga tactggcgtg agatcccgag
     1441 atagtagtat gtttttcatt cgtgagatcg atcgtttgga cgtcggagcc gctatcgcga
     1501 gcactgcgtg atctgctccc ctcggaagtc gtcgtcgcga gctcggcgtc cgagttcttc
     1561 gcggcgaagc gcgacgcgaa gaccgtgtcg atcatcgatc aggtcgtgct cggggacttc
     1621 aaccgggcgg cgctcgaatc cggtgttcgc gtcgatcaga ttggcccggt gatcacgatc
     1681 ttcgacgagc cgctgcagaa tacgattggc tgggtcgccg cgcacccctg gctgtctcat
     1741 gtcatgagct ctggcatgct ccgacacccg atcgcggccg agcaccttcg caatgtgatt
     1801 gcgactctca cgagcaaccg tccgcccagg ctcctcgact ggctggggcc cacgatcaac
     1861 gggcgccgcg tccggctcgc ccgcgccagc aagcgagtcg agcggctcga gcggctgacc
     1921 gcgtacctgg agtcccgggg agtcggcgag cgcacggtcc agcagctccg cgacgccgcc
     1981 gaggagctac tgaccaacgc gttctacgac gcaccggcgg ccgcgggggt gacacagccg
     2041 atctcccgca cgcgggatgt ccggctcccc gaggacagcg cgtgcgacat ggtctacgga
     2101 tgtcgcgacg atctcgcggt cgtgcgcgtg cgagatccgt tcggctcgct gtcgcgggcg
     2161 cggctcatcg aggtcctgat gcgatgcgcg cggacggaca tgcaggtcga ggtcgacgag
     2221 accatgggcg gagccggcct ggggctgtgg cggatcttca cggtggcctc gttcgtcgcc
     2281 atcgcggtgg tccgcaaccg tcataccgag gtgctggtcg ggatcggaaa gcgggtaccg
     2341 tccgcccggc ggccgttcgc ctttcatttt ttcttccgag aggatgcgcg tgcggcgccg
     2401 ggatggcgcc tgcaggacgc cgacaccacc cagccacagg tcaaccagtc ggtgaccatc
     2461 gtgaccaagt aggggcacgc agagatcgaa gtgtcaccca tgatccgtca ttccaagcct
     2521 cccagcgccg caccgccccc acccgcgcgc gccgcggcca ccgtcggtct ggcgaccaag
     2581 ggcgacgcgg tgctggtgag catctcgggc ctggtcgacg agcggttcac gggatttggc
     2641 aacctcgggg ccgcccggac cgtgatcctc gacgtgtccg ggatgacccg gatgacctcg
     2701 ttcggcgtgc ggcagtggct caaggcgatg gatgcgctgc ccaagtcgat caccgagctc
     2761 tacctgctgg gctgtccgac gttcttcgtc gaccagctca acatggtgct gaacttcggg
     2821 ggcccggcga agatcttgac ggtcatggcg cctcacacct gcccgtcgtg cggtgtcgag
     2881 tcgggcgcgc tgatcgacgt gctcgccgac cgcgccaacc tggtcaaggg cggcctgccc
     2941 gagaaggagt gcgcgcggtg cggcggcaag ctcgagtttg acgagacgcc cgagtcctac
     3001 ttcgcgttcg tgggcaagta cgcggcgtcg agcctcgggc cggcggccgc gcagctcctg
     3061 gccgcgcagg gcctgtacac ctcgtcggag agcgccagcg agaagccgcc gcggatcatc
     3121 aagctggtcc acggctcggt gacgtacttc cggatcatcg ggtcgatcgg gtcgatgttc
     3181 cgggcccggc cgttcctggt cggcgccgag ggcgaggtcg tcatcgacct cgcggaggtc
     3241 gagcggttcg actcggcggg cctgaacgaa tggcggcgcc tggtca
//
LOCUS       DASVOR010002251         3222 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_25733,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002251 DASVOR010000000
VERSION     DASVOR010002251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25733"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1194
                     /locus_tag="VF516_41640"
     CDS             <1..1194
                     /locus_tag="VF516_41640"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114327.1"
                     /translation="AEIEARYGPIRVPRIPRRAASEPGATRRGLGQPTALVTGFPAFT
                     ARRMIGQLLAAEPETRLYVLAPAPHAADAEQFLAALPDRAQAEVLVGDVCDMDLGLSS
                     AEYRALSRDLTWIHHLAGVYFMGTDDDTAHRVNVVGTRTVIDLARDASRLERVVHWST
                     AMVSGDRRGTFYEEDLEVGQKFHNAYERTKYEAERLVRAAMRQLPITVVRPSIIVGDS
                     RTGEIDKLDGPYYLMVVIATNASGVRLPLLGRGDAPLHLVPIDYVVEAAWHVARGETA
                     AGKTFHLVDPAPLSAREVFEAVAEHAHTEKPRGHIPRPLARAVLRTPGLSRLGRGPLA
                     FVDVLDHAVHYDQTNTAQALAGTAVRCPGLAEYLPALVRFVLDVTREAPPEPTDEVSD
                     PLDQV"
     gene            complement(1206..2207)
                     /locus_tag="VF516_41645"
     CDS             complement(1206..2207)
                     /locus_tag="VF516_41645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114328.1"
                     /translation="MWHRPVVSAVLGVVLVLGLGPVLAGCGFQAAASSGGGPRDAAID
                     GPGSGAVDGGDGPIAATDCWAHWMDGSVAIDGSTVKEITELSSTGHDLEPWISKDGLR
                     MYFSRDLTPPGHGDIYVTSRDTPTGMFAAPAPVANLNSTGHEGRAWLTTDELTLALST
                     AHDGPIDIHMITRAAGQMFGTPDNAHLAMVNAKGSMRFEPFLTDDLLRLYFSADSGPG
                     NKWQLWVAARSAPGDDFGTPSLVLNDNGMNYFAAALYQNEQLLVFSSFPMNATADLYY
                     ATRSGATGPFGTPVKIPTVNTSSSEAEAVLGHDGCELYFTSDRNAGRFHLFHARITK"
     gene            complement(2331..>3222)
                     /locus_tag="VF516_41650"
     CDS             complement(2331..>3222)
                     /locus_tag="VF516_41650"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEX8114329.1"
                     /translation="AVAAEDADAIAHGATGKGNDQVRFELAAYHFRPGIKIIAPWREW
                     KFRGRRELIEYTQQHKIPIEATADKPYSVDRNLLHTSYEGGVLEDPWREPDPAMFQRT
                     RDAREAPDAPRILELEVEHGDAVALDGERLGPVEMLTRLNQIAGEHGVGRVDLVENRF
                     VGMKSRGVYETPGGTVLHAARAAVESLTLDREVLRLRQDLMPRYAALVYNGFWFSPER
                     EALQKLIDDIAQSITGTVRLKLYKGALTVLGRKAPRSLYRPDIVTFEEDSVYDQRDAQ
                     GFIKLNALRLRIRSELDRRG"
BASE COUNT          449 a         1171 c         1137 g          465 t
ORIGIN      
        1 gccgagatcg aggcccggta cggcccgatc cgggtgccgc ggatcccgcg gcgcgccgcg
       61 tccgagcccg gcgcgacccg gcgcgggctc ggccagccca ccgcgctggt caccgggttc
      121 cccgcgttca ccgcgcggcg catgatcggc cagctgctcg ccgccgagcc cgagacccgg
      181 ctctacgtgc tggctcccgc gccgcacgcc gccgacgccg agcagttcct cgccgccctg
      241 cccgaccgcg cgcaggccga ggtcctggtc ggcgacgtct gcgacatgga cctcgggctg
      301 tcgagcgcgg agtaccgcgc gctgtcgcgc gatctgacct ggatccacca cctcgccggc
      361 gtgtacttca tgggcaccga cgacgacacc gcgcaccggg tcaacgtcgt cggcacgcgc
      421 acggtgatcg acctcgcgcg cgacgccagc cggctcgagc gcgtcgtcca ctggtcgacc
      481 gccatggtct cgggcgaccg caggggcacg ttctacgagg aagacctcga ggtcggccag
      541 aagttccaca acgcctacga gcgcaccaag tacgaggccg agcggctggt ccgcgcggcg
      601 atgcgccagc tgccgatcac cgtggtgcgg ccgtcgatca tcgtcggcga cagccggacc
      661 ggcgagatcg acaagctcga cggtccctac tacctgatgg tggtgatcgc gaccaacgcc
      721 tcgggcgtgc ggctgccgct gctcggccgc ggcgacgccc cgctccacct ggtcccgatc
      781 gactacgtgg tcgaggccgc ctggcacgtc gcgcgcggcg agaccgccgc cggcaagacg
      841 ttccacctgg tcgatccggc gccgctgtcg gcgcgcgagg tgttcgaggc ggtggccgag
      901 cacgcccaca ccgagaagcc gcgcggccac atcccgcggc cgctcgcgcg cgcggtgctg
      961 cgcaccccgg ggctgtcgcg gctcggccgc ggcccgctgg cgttcgtcga cgtgctcgac
     1021 cacgcggtgc actacgacca gaccaacacc gcgcaggcgc tcgccggcac cgccgtgcgc
     1081 tgcccggggc tcgccgagta cctgccggcc ctggtgcgct tcgtgctcga cgtcacccgc
     1141 gaggcgccgc ccgagcccac cgacgaggtc agcgacccgc tcgatcaggt gtagcggcgg
     1201 cgccgctact tcgtgatccg ggcgtggaac aggtgaaagc ggccggcgtt gcgatccgac
     1261 gtgaagtaca gctcgcagcc gtcgtggccg agcaccgcct cggcctcgct ggagctggtg
     1321 ttgacggtgg ggatcttgac cggcgtaccg aaagggccgg tggcgcccga gcgggtcgcg
     1381 tagtacaggt ccgcggtcgc gttcatggga aacgagctga acacgagcag ctgctcgttc
     1441 tgatacagcg cggcagcgaa gtagttcatg ccgttgtcgt tgaggacgag cgacggggtg
     1501 ccgaagtcgt cgcccggcgc ggatcgcgcc gcgacccaca gctgccactt gttgcccggt
     1561 cccgagtccg ccgagaagta cagccgcaga aggtcatcgg tgaggaacgg ttcgaaccgc
     1621 atggacccct tggcgttgac catcgccagg tgcgcattgt ccggcgtgcc gaacatctga
     1681 ccggcggcac gcgtgatcat gtggatgtcg atcggcccat catgcgccgt cgacagcgcg
     1741 agggtcagct cgtccgtggt cagccaggcg cgcccctcgt ggccggtgct gttcaggttg
     1801 gcgaccgggg ccggcgcggc aaacatcccc gtcggggtgt cacggctggt gacgtagatg
     1861 tcgccatgcc ccggcggcgt gagatcgcgc gagaagtaca tccggagacc gtccttcgag
     1921 atccagggct cgaggtcgtg tccggtgctc gacagctccg tgatctcctt caccgtcgag
     1981 ccatcgatgg cgaccgagcc gtccatccag tgcgcccagc agtccgtcgc cgcgatcggc
     2041 ccatcgcccc cgtcgaccgc gccgctgccg gggccgtcga tcgcggcgtc gcggggaccg
     2101 ccgccggacg aggcggccgc ttgaaagccg caccccgcga ggacgggacc gaggccgagg
     2161 accaggacga cgccgaggac cgcgctgacc acaggccggt gccacacccg ccggatccta
     2221 tccaattccg gccggatttc acgccgcccg gcggcgcggc ctgcgctttg tcacagcctc
     2281 gggcggatca ccgcgagacg gacgccggct acccgggacg gcgcggacgt tcagccccgg
     2341 cggtcgagct cgctgcggat ccgcaggcgc agcgcattga gcttgatgaa gccctgggcg
     2401 tcgcgctggt cgtagaccga gtcctcctcg aacgtcacga tatccgggcg gtacaggctg
     2461 cgcggcgcct tgcggccgag cacggtcagc gcgcccttgt agagcttgag ccgcaccgtg
     2521 ccggtgatcg actgggcgat gtcatcgatc agcttctgca gcgcctcgcg ctcgggcgag
     2581 aaccagaacc cgttgtagac cagcgccgca taacgcggca tcaggtcctg gcgcagccgc
     2641 aggacctcgc gatcgagggt cagcgactcg accgcggcgc gcgccgcgtg cagcaccgtc
     2701 ccgcccgggg tctcgtagac gccgcgcgac ttcatgccga cgaagcggtt ctcgaccagg
     2761 tcgacccggc cgacgccgtg ctcgccggcg atctggttga gccgggtcag catctcgacc
     2821 gggccgagcc gctcgccgtc gagcgcgacc gcgtcgccgt gctcgacctc gagctcgagg
     2881 atccgcggcg cgtcgggtgc ctcgcgcgca tcgcgggtgc gctggaacat cgccggatcg
     2941 ggctcgcgcc aagggtcctc gagcaccccg ccctcgtagc tggtgtgcag gaggttgcgg
     3001 tcgaccgagt acggcttgtc ggcggtggcc tcgatcggga tcttgtgctg ctgggtgtac
     3061 tcgatcagct cgcgccggcc gcggaacttc cactcgcgcc acggcgcgat gatcttgatc
     3121 cccggccgga agtggtacgc cgcgagctcg aaccggacct ggtcgttgcc cttgccggtc
     3181 gcgccgtgcg cgatcgcgtc ggcgtcctcg gcggcgaccg cg
//
LOCUS       DASVOR010002252         4449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_432918,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002252 DASVOR010000000
VERSION     DASVOR010002252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4449
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432918"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="VF516_41655"
     CDS             complement(<1..605)
                     /locus_tag="VF516_41655"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEX8114330.1"
                     /translation="MASLAATFGRGAMTNGWVDIKNTDVILAMGGNPAENHPVGFKWF
                     MEASRGRGAKLVAVDPRFTRTAAVADFYAPIRPGTDIAFLLGIIRYAIDTKRFHEEYV
                     KLHTNASYIIGDKYEFKVEDGLFSGFDPEKKEYDKSNWAYAADDASKAYRVDPTLSDE
                     RCAFQLLKKHVERYTPEMVERICGTPKAAFLKVAEMVTSTG"
     gene            complement(618..1190)
                     /locus_tag="VF516_41660"
     CDS             complement(618..1190)
                     /locus_tag="VF516_41660"
                     /inference="COORDINATES: protein motif:HMM:NF016750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114331.1"
                     /translation="MNRRTFFKLGAVVSGGAALGFDLKSARAEMRELKIARTTETRST
                     CPYCAVSCGVIIHTLGDKAKNVSPAVVHVEGDPDHPISRGSLCPKGAALKGDIHHANR
                     LTKPQVRRPGSDKWEDISWDEAIAGIAKHVKDTRDRTFQEKNAKEETVNRTPGIAWIG
                     GCTDTNEFNFLQWKALTSWGVPYRDTQARV"
     gene            1417..>4449
                     /gene="nifJ"
                     /locus_tag="VF516_41665"
     CDS             1417..>4449
                     /gene="nifJ"
                     /locus_tag="VF516_41665"
                     /inference="COORDINATES: protein motif:HMM:TIGR02176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEX8114332.1"
                     /translation="MAERFAESEQFARDAAGRADPAGIAAATQNTVRADERRSSQAKT
                     LDANEAVARIAYRLSEVIAIYPITPASAMGEHADAWAAEGRPNLWGTVPDVVEMQSEA
                     GAAGAVHGALQTGALATTFTASQGLLLMLPDLFKIAGELTAFCLHVAARTIATHALSI
                     FGDHSDVMAARTTGLALLASGSAQEAQDLAAIAHAATLAARIPFLHFFDGFRTSHEIT
                     KVELLGDDALRALIERSEMGGGDPAGSAGGAGGKAPRGIDGAAIAAHRRRALDPDRPV
                     IRGTSQNPDTYFQAREAIEPFYAACPGIVDAAMARFAELTGRRYRLFDYAGHPEAERV
                     IVMMGSGAECAHETVDHLVARGERVGVVKVRLYRPFSLRHLLAALPATARAIAVLDRT
                     KEPGSIGEPLLLDVTQALVEAVATGERASLPRVIGGRYGLSSKEFTPAMVAAVFDELG
                     RAAPRRRFTVGIRDDVSQSSLAWDPELDIEPDDVSRAVFFGLGSDGTVSANKATIKII
                     GEHTGGFAQGHFEYDSKKSGSTTISHLRFGPRPIRSTYRIRRAQLVAIHDPEALERRD
                     VLAAAAPGAIVLINTPVAPDRVWDALPREVQDQLIARRCRLFAIDGYGVAERAGLGRR
                     INIVMQACFFALSKVLPIDQALGHIRDSIEATFGKRGPEVVRRNLAALDATLEALREV
                     AVPAAATTERHRLPVVPEAAPDFVRRVTRLLLEGRGDELPVSAFPPDGTWPTGTSKFE
                     KRAIALDIPIWQPDVCIQCNFCSMICPHAAIQTRVFAPEALAGAPAGFRSQPERMDKA
                     HKGMQFTVQVAPEDCTGCGLCIEICPAKDKKQPRRKALMSEPLAAHREPLRAAFAFFE
                     TIPSAPLDGLKIDKRTAVLRLPLFEFSGACAGCGETPYIRLLTQLYGDRLVIANATGC
                     SSIYGGNLPTTPYTTDRAGRGPAWANSLFEDNAEFGLGMRLSLDVHAGQARALLTRLA
                     PRLPGALVDGLLSSPGDDAWIAAQRDRLVELARALDRDG"
BASE COUNT          633 a         1621 c         1515 g          680 t
ORIGIN      
        1 ttgccggtcg aggtcaccat ctcggcgacc ttgaggaacg ccgccttggg cgtgccgcag
       61 atccgctcga ccatctccgg cgtgtagcgc tcgacgtgct tcttgagcag ctggaacgcg
      121 cagcgctcgt cgctcagcgt cggatcgacc ctgtacgcct tgctcgcgtc atcggccgcg
      181 tacgcccagt tgctcttgtc gtactccttc ttctccggat cgaagcccga gaacaggccg
      241 tcctcgacct tgaactcgta cttgtcgccg atgatgtacg aggcgttggt gtggagcttg
      301 acgtactcct cgtgaaagcg cttggtgtcg atcgcgtacc ggatgatccc gagcaggaac
      361 gcgatgtcgg tgccgggccg gatcggtgcg tagaagtccg cgaccgcggc cgtccgcgtg
      421 aaccgcgggt cgacggcgac cagcttggcg ccgcgcccgc ggctcgcctc catgaaccac
      481 ttgaagccga cgggatggtt ttccgcaggg ttcccaccca tggccaggat gacatccgta
      541 ttcttgatgt cgacccagcc attggtcatc gccccgcggc cgaacgtggc agccaaactg
      601 gccaccgtgg gaccgtgtca aacacgggct tgtgtatctc gatacggcac tccccaggac
      661 gtcagcgctt tccactgcag gaagttgaac tcgttggtgt ccgtgcagcc gccgatccac
      721 gcgatccccg gcgtgcgatt gaccgtctct tccttggcgt tcttctcctg gaacgtgcga
      781 tcgcgcgtgt ccttgacgtg cttggcgatc ccggcgatcg cctcgtccca cgagatgtct
      841 tcccacttgt cgctgccggg ccgccggacc tgcggcttgg tcagccggtt ggcgtggtgg
      901 atatcgccct tgagcgcggc gcccttcgga cacaggctgc cgcggctgat cggatgatca
      961 ggatcgccct cgacatggac gaccgccgga gacacgttct tcgccttgtc gccgagcgtg
     1021 tggatgatga ccccacatga gactgcacag tacggacacg tactacgcgt ctcggtcgtg
     1081 cgcgcgatct tgagctcgcg catctcggca cgagcactct tcagatcgaa tcccagcgcg
     1141 gcaccgccgc tcacgactgc cccgagtttg aagaaggtcc ggcgattcat cgggacacct
     1201 ccagcgtttc gagcatcagg tcacagtgcc ccgtcggttg tcaagtcgcc gtaacgaaac
     1261 acactgcgga ccaacacagc ggtattggca ccgtgcccac gacgcgacgc ggcgcgacgc
     1321 gcggattcgc ggccgcgcgc ccgggccgca gggccaaccg gtgcggcgat cgccgatgaa
     1381 gcggcgcggc acgaaacctg cgacgtggac ctcgccatgg ctgaacggtt tgcagagagc
     1441 gaacaatttg cgcgcgatgc cgcggggcgc gcagatccag cggggatcgc tgccgcgacg
     1501 caaaacacgg tacgggccga cgagcggcgc agctcgcagg cgaagacgct cgacgccaac
     1561 gaggcggtcg cgcggatcgc ctaccggctg agcgaggtca tcgcgatcta tccgatcacg
     1621 ccggcgtcgg cgatgggcga gcacgccgat gcgtgggcgg ccgagggccg gcccaacctg
     1681 tggggcaccg tccccgacgt ggtcgagatg cagtccgagg ccggcgccgc gggcgcggtg
     1741 cacggtgcgc tgcagaccgg cgcgctggcg accacgttca ccgcgtcgca gggcctgctg
     1801 ctcatgctcc ccgatctgtt caagatcgcc ggcgagctca ccgcgttctg cctgcacgtc
     1861 gcggcgcgca cgatcgcgac ccacgcgctg tcgatcttcg gcgatcactc cgacgtgatg
     1921 gcggcgcgca cgaccggcct ggcgctgctc gcctcgggct cggcccagga ggcccaggac
     1981 ctcgcggcga tcgcgcacgc cgcgacgctg gccgcgcgga tcccgttcct ccacttcttc
     2041 gacgggttcc gcacctcgca cgagatcacc aaggtcgagc tgctcggcga cgacgcgctg
     2101 cgcgcgctga tcgagcgaag cgagatgggg ggtggggacc ctgccggctc tgccggcggg
     2161 gcggggggca aagccccccg tgggatcgac ggggccgcga tcgccgcgca tcgccgccgc
     2221 gcgctcgacc ccgaccgccc ggtgatccgc ggcacgtcgc agaacccgga cacctacttc
     2281 caggcccgcg aggcgatcga gccgttctac gccgcgtgcc cggggatcgt cgacgccgcg
     2341 atggcgcggt tcgccgagct caccggccgg cgctaccggc tgttcgacta cgccggccac
     2401 cccgaggccg agcgcgtgat cgtgatgatg ggctcgggcg ccgagtgcgc gcacgagacc
     2461 gtcgaccacc tggtcgcgcg cggcgagcgg gtcggcgtgg tcaaggtccg gctgtatcgg
     2521 ccattctcgc tccgccacct gctggcggcg ctgccggcga ccgcgcgcgc catcgcggtg
     2581 ctcgaccgca ccaaggagcc ggggtcgatc ggcgagccgc tgctcctcga cgtcacccag
     2641 gcgctggtcg aggcggtcgc gaccggcgag cgggcgagcc tgccgcgcgt gatcggcggt
     2701 cgctacggcc tgtcgtcgaa ggagttcacc cccgcgatgg tcgcggcggt gttcgacgag
     2761 ctcggccgcg ccgcgccgcg ccggcggttc accgtcggca tccgcgacga cgtctcgcag
     2821 tcgtcgctgg cctgggatcc cgagctcgac atcgagcccg acgacgtgtc gcgcgcggtg
     2881 ttcttcggcc tgggctcgga cggcacggtg tcggccaaca aggcgacgat caagatcatc
     2941 ggcgagcaca ccggcgggtt cgcccagggc cacttcgagt acgactccaa gaagtcgggc
     3001 tcgacgacga tctcccacct gcggttcggg ccgcggccga tccgctcgac ctaccggatc
     3061 cggcgcgccc agctggtcgc gatccacgac cccgaggcgc tggagcgccg cgacgtgctg
     3121 gccgcggcgg cgcccggcgc gatcgtgctg atcaacacgc cggtcgcgcc cgaccgggtg
     3181 tgggacgcgc tgccccgcga ggtccaggat cagctgatcg ctcggcgctg ccggctgttc
     3241 gcgatcgacg gctacggcgt cgccgagcgc gccgggctcg gccgccggat caacatcgtc
     3301 atgcaggcgt gcttcttcgc gctctcgaag gtgctgccga tcgaccaggc gctgggccat
     3361 atccgggaca gcatcgaggc aacgttcggc aagcgcggcc ccgaggtcgt gaggcgcaac
     3421 ctcgccgcgc tcgacgctac cctcgaggcg ctccgggaag tcgccgtgcc cgcggcggcg
     3481 accaccgagc gccaccggct gccggtcgtg cccgaggccg cgcccgactt cgtgcggcgc
     3541 gtgacccggc tcctgctcga gggccgcggc gacgagctgc cggtcagcgc gttcccaccc
     3601 gacggcacct ggccgaccgg caccagcaag ttcgagaagc gcgcgatcgc gctcgacatc
     3661 ccgatctggc agcccgacgt ctgcatccag tgcaacttct gctcgatgat ctgcccgcac
     3721 gcggcgatcc agacccgggt gttcgcgccc gaggcgctgg ccggcgcccc cgcgggcttc
     3781 cgctcgcagc ccgagcggat ggacaaggcg cacaagggca tgcagttcac cgtgcaggtc
     3841 gcgccggaag actgcaccgg ctgcggcctg tgcatcgaga tctgcccggc caaggacaag
     3901 aagcagccgc ggcgcaaggc gctgatgtcc gagccgctcg ccgcccaccg cgagccccta
     3961 cgcgcggcgt tcgcgttctt cgagaccatc ccgtcggcgc cgctcgacgg cctcaagatc
     4021 gacaagcgca ccgcggtgct ccggctgccg ctgttcgagt tctccggcgc gtgcgccggc
     4081 tgcggcgaga ccccgtacat ccggctgctc acccagctct acggcgatcg cctggtgatc
     4141 gccaatgcca ccggctgctc gtcgatctac ggcggcaacc tgccgaccac gccgtacacc
     4201 accgaccggg ccggccgcgg cccggcgtgg gccaactcgc tgttcgagga caacgccgag
     4261 ttcggcctcg gcatgcggct gtcgctcgac gtccacgccg gccaggcccg cgcgctcttg
     4321 acccggctcg cgccccggct gcccggcgcg ctggtcgacg gcctcctgtc ttcgcccggc
     4381 gacgacgcct ggatcgccgc gcagcgcgac cggctggtcg agctcgcccg cgcgctcgac
     4441 cgcgacggc
//
LOCUS       DASVOR010002253        10334 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_375097,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002253 DASVOR010000000
VERSION     DASVOR010002253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375097"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1536
                     /locus_tag="VF516_41670"
     CDS             <1..1536
                     /locus_tag="VF516_41670"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEX8114333.1"
                     /translation="LAGLAEAGDVLRRFLARPAEHAPGPLLALLDQLTAMARGWIDAL
                     IAADGEARRRWYMVDLVLAAMRGVIRDRIALDPRGFDAINHLEFRDWLRRHGASELTL
                     DSDHLRGGIYDLSFAYVDGDPARPAIGAGDAMRGACRMFFTYRGSFFWKMRGGMGDVV
                     FAPLYELLRRRGVRFEFFHRLDNVRLADDGSHVAALDMSVQAEPRGDYHPLVEVGGMP
                     CWPAEPRYDQLIDGDALRAAGWQPESFWDPRRAAARTLRVGHDFDLVVLGVGLGAIPH
                     VCREIVARDARWRAMTEHVATVATQAFQLWLHADLAELGWPGPSPSVSGFAKPFDTWA
                     DMPHLIDVEAWRHPPRSIAYFCGALALPPGALDDPDPLAAQRERVRRAAIDFLDRQIQ
                     PLWPRAVRPGGGFRWELLADAASDDGAAPASAGPERFATQFWSANVHPSDRYVQSLPG
                     TAAYRISPLDNTYDNLTIAGDWTACGLNVGCVEAAVISGRLAAHAISQQPALADIIGY
                     DHP"
     gene            1893..2945
                     /locus_tag="VF516_41675"
     CDS             1893..2945
                     /locus_tag="VF516_41675"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEX8114334.1"
                     /translation="MGERQQIFWGTDAVRERHQFDAARLEDWMRVHVDGFRGHLAVAQ
                     FKGGQSNPTYLVEAGGTRYVVRRKPPGHLLPSAHAVDREYRVLTALQGTGFPVPRTYG
                     LCTDDSVIGTWFYVMEFVEGRVLWEPALPDLSPRERFAIYDSLCEVLARLHALDPRKL
                     GLEDFGRSGNYFARQIARWSKQYCASVDRPHTEMDALNDWLPRTIPPGDETTIIHGDF
                     KLDNTIIHPTEPRVVAVLDWEISTLGHPLGDFTYLCLPWFRSGPFSELDLAAHGLPTL
                     DAYAASYCARAGRPRIESFAWYEAFHLFRSACILQGILGRVRDGTAASDHPSRIAEGI
                     GPLAKAAWAVAKELGA"
     gene            2968..3102
                     /locus_tag="VF516_41680"
     CDS             2968..3102
                     /locus_tag="VF516_41680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114335.1"
                     /translation="MTPALTVPGHRGEVGRHSGVGRRLAAHAISQQPVLADIIGYDHA
                     "
     gene            complement(3139..3399)
                     /locus_tag="VF516_41685"
     CDS             complement(3139..3399)
                     /locus_tag="VF516_41685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114336.1"
                     /translation="MALLPILVGLGQAAVPPLVKLLDDPGPIFFEGSREAMTGNKLKL
                     RVKDFAAYYLGKIENIAVPLQPDHPARDAEIEKLKAKLAAKK"
     gene            complement(3963..4214)
                     /locus_tag="VF516_41690"
     CDS             complement(3963..4214)
                     /locus_tag="VF516_41690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114337.1"
                     /translation="MARSVRGDLQPLPAGVDLNLAMRRSVRVGTRSGLPVEGIGIFSD
                     AGYPPTRRDLISDNDDLVAYCAGLARRAARRLASARHAT"
     gene            complement(4365..5171)
                     /locus_tag="VF516_41695"
     CDS             complement(4365..5171)
                     /locus_tag="VF516_41695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114338.1"
                     /translation="MSLFDDASSAAQFLQRSPLLAMLRDSITVTSQADRADIARGLTA
                     AIRDYYVHLPLKRSSLGIDPVRELELLVDDVAYIPTEDPFFARVIEILCRMRDRHTTL
                     LLPAPRAQAIAFLPFAVESYFDGAKQRHLIVSKLLCDVGETDFAPGVELTHWNGMSVR
                     RHVEDFSWQTDGANPFARIALALRTLTVRPLGYMVPPAEDWVTLTYFTEKGIKSIALP
                     WRVYFADGSSGAGTAARTLIGASARTRAARSQRPNECCSCSARAGRRSRR"
     gene            complement(5181..5663)
                     /locus_tag="VF516_41700"
     CDS             complement(5181..5663)
                     /locus_tag="VF516_41700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SagB family peptide dehydrogenase"
                     /protein_id="HEX8114339.1"
                     /translation="MPRFRGHLPSLSRHDGGVHESQGNDELELYLAVHACRDLERGLC
                     YYDPLRHALSWVAPASSELDALLHDAWISSARQYTPQILVTIASRFQRVAWKYAGMAY
                     ATQLKNAGVLIQTMYLVATAMGLAPCALGLGNAERFCRLTGTDHLHEGSIAEFMIGTP
                     "
     gene            complement(5620..6474)
                     /locus_tag="VF516_41705"
     CDS             complement(5620..6474)
                     /locus_tag="VF516_41705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOMM precursor leader peptide-binding protein"
                     /protein_id="HEX8114340.1"
                     /translation="MHAKIYLAIVDDYLRPSLFELNRACLARGVPLFLVKPVGVRAWI
                     GPLVVAGQTACWRCLEYRLRANREVEAYIERRTGKRMPLALPRARVPLFERHASSVAI
                     TQLVRFLTTGANPGLGSHVLVTDLTSLTFERHPIVRRPQCPDCGNPRLGTRAGLPVQL
                     TAQRVAGESTSGMRRDEPEAVLQRYQHHVSPLTGIVREMTPSAWHGAGPLRVYMAGHN
                     FALKNDHLFLLKDGLRNNSSGKGRTEAQARTSALCEALERYSGVFRGDEPRRPAPFSW
                     TPPIPLPS"
     gene            complement(6665..7591)
                     /locus_tag="VF516_41710"
     CDS             complement(6665..7591)
                     /locus_tag="VF516_41710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114341.1"
                     /translation="MSSALEQPNGGFDTSDEAPEFGAQTDFTAAAIESDSAATDTLAG
                     DPATTAIDTASATAGFRMMLVWGKIPADKDATVSRDWTGSLKVSRGGLIVRRTIAFED
                     RTDKLLPRAALDAVSFQSVTRPYVDGLALTVLDPPSPTATGSQTLTYTSADGATTYTF
                     DLSQLAAGPIAVDAGDGFKMVAIGVHRQAAACDRGFMRGRWHALSAHLGRFLGVVTDE
                     NGAPVGHVRGVYGQRRNGDSVLFGKFIDREGHFRGLLGGTFADGQYQARWIDRQGDHG
                     TAHGAYFEGQAATSGHFLGRWAESSCAADPTP"
     gene            7793..8272
                     /locus_tag="VF516_41715"
     CDS             7793..8272
                     /locus_tag="VF516_41715"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114342.1"
                     /translation="MPAGSAAADQAVPRGAGVYGSGHRRGRCRSGRAWPAVDFAPRNF
                     YGPRPWKDSVRTSLDRHFGAGPRLPTVTSGAIASSDRLIKEVEILDLWRKQARQIRAV
                     EMESAGLYKAAHGRVPFLAIRGISDIVGFKRDPGWTSYACHTAAAFTRAFLRAARWR"
     gene            complement(8328..8933)
                     /locus_tag="VF516_41720"
     CDS             complement(8328..8933)
                     /locus_tag="VF516_41720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114343.1"
                     /translation="MNAATGETDEGAHIDQADYNRNPLYAAEGAPAGDTRLDQTTPDP
                     DVTKKNDWGRHGFRYTDASGALKEQDALLNDETGLNNGATDSAQIYETTALAIKGNQK
                     DSYYGSVEWGWRTDSGGAFTKLPLKVISQGVPSSTFMKSAELWNSGTTASGASTLDLL
                     RGQPRGDDRQQAEAPRQGLRGVLPRQDREHRRAAATGSPGA"
     gene            9010..9378
                     /locus_tag="VF516_41725"
     CDS             9010..9378
                     /locus_tag="VF516_41725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114344.1"
                     /translation="MIVFAVCVDADELGIDVGLGLEPQIDLDAAAVAGSIDRVVVVEP
                     LAIGGVDLALHRAATGVRSAATGVGVEQTLGGLALDHQIGDPVGVLLVRIAVAPDAAS
                     EVNARALLDDVGRFVRREVQ"
     gene            complement(10077..>10334)
                     /locus_tag="VF516_41730"
     CDS             complement(10077..>10334)
                     /locus_tag="VF516_41730"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8114345.1"
                     /translation="DAEAAGTAAPADAAATFGGITAKHKGDRYLNVLIDGQMVGPTPL
                     FNRRLATGSHVVELVDPRSNDVVARRSVKVEAGETVTVTEP"
BASE COUNT         1491 a         3639 c         3571 g         1633 t
ORIGIN      
        1 ctggccgggc tcgccgaggc cggcgacgtc ctgcgccggt tcttggcccg ccccgccgag
       61 cacgcacccg gtccgctgct ggcgctgctc gatcagctga ccgccatggc ccggggctgg
      121 atcgacgcgc tgatcgccgc cgacggcgag gcccgccgcc gctggtacat ggtcgacctg
      181 gtgctcgcgg cgatgcgcgg cgtgatccgc gaccgcatcg cgctcgatcc gcgcggcttc
      241 gacgccatca accacctcga gttccgcgac tggctgcgcc ggcacggcgc gtccgagctc
      301 accctcgact ccgaccacct gcgcggcggc atctacgatc tgtcgttcgc ctacgtcgac
      361 ggcgatccgg cgcggcccgc gatcggcgcc ggggacgcca tgcgcggcgc ctgccgcatg
      421 ttcttcacct accgcggctc gttcttctgg aagatgcgag gcggcatggg cgacgtcgtg
      481 ttcgcgccgc tctacgagct attgcgccgg cgcggcgtgc gcttcgagtt cttccaccgc
      541 ctcgacaacg tccggctcgc ggacgacggc tcccacgtcg cggccctcga catgtcggtc
      601 caggccgagc cccgcggcga ctaccacccg ctcgtcgagg tcggcggcat gccgtgctgg
      661 ccggccgagc cccgctacga tcagctgatc gacggcgacg ccctgcgcgc cgccggctgg
      721 cagcccgagt cgttctggga cccgcgccgc gccgctgcgc gcaccctgcg cgtcggccac
      781 gacttcgacc tcgtcgtcct cggcgtcggc ctgggcgcca ttccccacgt gtgccgcgag
      841 atcgtcgcgc gcgacgcgcg ctggcgggcg atgaccgagc acgtcgccac cgtcgcgacc
      901 caggcgttcc agctctggct ccacgccgac ctggccgagc tcggctggcc cggcccgtcg
      961 ccgagcgtct ccggcttcgc caagccgttc gacacctggg ccgacatgcc gcacctgatc
     1021 gacgtcgagg cctggcgcca tccgccgcgc tcgatcgcct acttctgcgg cgcgctggcc
     1081 ctgccgccgg gcgcgctcga cgatcccgat cccctcgccg cgcagcgcga gcgcgtccgc
     1141 cgggccgcga tcgacttcct cgatcgccag atccagccgc tgtggccgcg cgcggtgcgc
     1201 ccgggcggcg gcttccgctg ggagctgctc gccgacgccg ccagcgacga cggtgccgcg
     1261 cccgccagcg ccggccccga gcggttcgcg acccagttct ggtcggccaa cgtccacccg
     1321 tccgatcgct acgtccagtc gctccccggc accgccgcct accggatctc gccgctcgac
     1381 aacacctacg acaacctgac catcgccggc gactggaccg cgtgcggcct caacgtcggc
     1441 tgcgtcgagg ccgcggtcat ctcgggccgg ctcgccgccc acgccatctc acagcagccc
     1501 gcgctcgccg acatcatcgg ctacgaccat ccgtgagcgg cgtcagattt gacgcatacg
     1561 atcatatgta atacggccgt atgcatgaac gtcgaccggc tctcggccct gacattgcat
     1621 ccccatgcgg tcccgacgtg agctggtact cgagaatgcg gtggacgcat tccgccgctc
     1681 cggatatctt cgcggcgatg aggcttgtgc tggtcaagtt cctgattgct cggggtcgtc
     1741 ggcgccgtcg gcaccaactg gaccgtgctg tgcggcagcg gcaagtgcga ggacaccgct
     1801 ccgctctact gcttcgagca gtgatcagct gcatcagcgc atttgagcgg taggacgttg
     1861 ggatctacga ccggtaagat cgggccgcga gcatgggcga acgacagcaa atcttctggg
     1921 gcaccgacgc agttcgcgag cgccaccagt tcgacgcggc gcgtctcgag gactggatgc
     1981 gcgtacacgt cgacggtttc cgcgggcacc tcgcggtcgc acagttcaag ggcggccaga
     2041 gcaatccgac ctacctcgtc gaggccggcg gcacgcgcta cgtggttcgc cgcaagccgc
     2101 cgggacatct cctgccgtcg gcgcacgcgg tcgaccgcga gtaccgcgtg ctcaccgcgc
     2161 tccagggcac gggcttcccg gtgccgcgca cctatggcct ttgcaccgat gacagcgtga
     2221 tcggcacctg gttctacgtc atggagttcg tcgagggtcg cgtcctctgg gagccggcgc
     2281 tgccggatct ttcgccgcgc gagcgcttcg cgatctacga ctcgctgtgc gaggtgctgg
     2341 cccggcttca tgcgctcgac ccccgcaagc tcggactcga ggacttcggc cggtcgggga
     2401 attacttcgc gaggcagatc gcgcgctggt ccaagcagta ttgcgcgtcg gtcgaccggc
     2461 cgcacaccga gatggacgcg ctgaacgact ggctgccgcg caccatcccg ccgggcgacg
     2521 agacgacgat catccacggc gacttcaagc tcgacaacac catcattcac cccaccgagc
     2581 cgcgcgtcgt cgccgtgctc gactgggaga tctcgacgct cggtcacccg ctcggcgatt
     2641 tcacctacct ctgcctgccg tggttccgct cgggtccgtt cagcgagctc gacctcgccg
     2701 cgcacggcct ccccaccctc gacgcgtatg ctgcctcgta ctgcgcgcgc gccggccgcc
     2761 cgcgcatcga gtccttcgcc tggtatgagg ccttccacct gtttcgctcg gcctgcatcc
     2821 tccagggcat cctcgggcgc gtgcgcgacg gcacggccgc gagcgaccac ccgtcgcgca
     2881 tcgccgaggg catcggcccg ctggcaaagg ccgcgtgggc ggtcgccaag gagctcggcg
     2941 catgaggggt tgcggccgac gacggcgatg acgcccgcgc tcacggtccc cggacatcgc
     3001 ggcgaggtgg gtcgacacag cggggtcggg cggcggctcg ccgcccacgc catctcgcag
     3061 cagcccgtgc tcgccgacat catcggctac gaccacgcgt gagcccggat ccgctgtggc
     3121 cagcaccggg cccagccgtc acttcttcgc ggccagcttg gccttcagct tctcgatctc
     3181 tgcgtcgcgc gccgggtgat ccggttgcag tggtacggcg atgttctcga tcttgccgag
     3241 gtagtacgcc gcgaagtcct tgacgcggag cttcagcttg ttgccggtca tcgcctcgcg
     3301 gctgccctcg aagaagatcg gcccagggtc atcgagcagc ttgaccagcg gcggcaccgc
     3361 ggcctggccc aggccgacca ggatcgggag cagggccacg atgtcgtccc cgccgcgccc
     3421 tcctgcccgg gaggacgtgc tgctcaccgc gctggaggct cggctgcgag gcgatgggcc
     3481 tgaaccgggc gctgggtgac gcggcgacca ccgcgaggcg tctcgagcag ctccagagaa
     3541 acgctggtct tcctggttgc acgttccttg cgcgagtgac aacacaagcg tgttccgcac
     3601 tatcggcttg acgtcgacga tccgccgtcc gatcctacaa ctccatacca ggaggtccac
     3661 atgcgaatgt tgatggcaat tgcgtttggg tgcgcgctga tgatcggagt cactcgcgcc
     3721 gcgagctccg gaccgaccgc gcgcgaagtg cccccgccca cgcagggcgc cacccctgca
     3781 gaccgctgcg agctcgacaa cgcgagccag gacaggtgcg cgaacaggcc gagctgcacc
     3841 gaggcgtcgc aatgcacggc gttctgcgcc ggcggtgccc cggcgtgttt ccagggatgc
     3901 tgcagctgcg ccagctgaca tcggctcgat caggggcgcg gtccacctga cccggacggt
     3961 cgctacgtag cgtgccgagc gctggctaga cggcgcgcgg cgcggcgagc aagaccggcg
     4021 cagtatgcga cgaggtcgtc gttgtcgctg atgaggtcgc gccgcgtcgg cgggtacccc
     4081 gcgtcggaga agataccgat gccctcgacg ggcagtcccg aacgcgtgcc gacgcggacc
     4141 gagcgccgca tcgcgaggtt gagatcgaca ccggccggaa gcggttgcag gtcgcccctg
     4201 accgagcggg ccatctgtgc gagcgtcgaa agcgaccaca cattcgcgcc gccggcgccg
     4261 gcgtgaacac ctcgccggtc tcgagcgcca tgtcgagcga ccgcttccac ggcaggaatc
     4321 gggaagcgcg ccgagccgct gcacctcggg cgtgttgcga aagctcaccg gcgcgaacgt
     4381 cggccagctc tggctgaaca gctgcaacat tcgttcggcc gctgggatcg agccgcccgg
     4441 gttcgcgcgg acgcgccgat caacgtgcgc gcggcggtcc cggcgccgct gctgccgtcg
     4501 gcgaagtaca cgcgccaagg cagagcgatg ctcttgatgc ccttttcggt gaagtaggtc
     4561 agcgtcaccc agtcctcggc cggcggcacc atgtagccga gcgggcgaac ggtcagcgtg
     4621 cgcagcgcga gcgcgatgcg ggcgaacggg ttcgcaccat cggtctgcca gctgaagtcc
     4681 tcgacgtggc ggcgcaccga catcccgttc cagtgcgtga gctcgacgcc gggcgcgaag
     4741 tctgtttcgc ccacgtcgca cagcagcttg ctgacgatca ggtgccgctg cttcgcgccg
     4801 tcgaagtagc tctccaccgc aaacggcaag aacgcgatcg cctgcgcgcg tggcgccggc
     4861 aacagcagcg tcgtgtgacg gtcgcgcatc cggcacagga tctcgatgac gcgcgcgaag
     4921 aacgggtcct cggtcgggat gtacgcgacg tcgtcaacga gcagctcgag ctcgcgcacc
     4981 ggatcgatcc cgagcgagct gcgcttgagc ggaaggtgga cgtagtaatc gcggatcgcc
     5041 gccgtcaatc cgcgcgcgat gtccgcacga tcggcttgcg aggtgaccgt gatgctgtcg
     5101 cgcagcatcg cgagcaatgg cgagcgttgc aagaactgcg cggcggagga ggcatcgtcg
     5161 aagagggaca tggatctccg ctagggtgtg ccgatcatga actcggcgat cgagccctcg
     5221 tgcaggtggt cggtgccggt gagccggcag aatcgctcgg cgttgccgag gccgagcgcg
     5281 cacggcgcga gacccatcgc ggtggcgacg aggtacatcg tctggatcag cacgccagcg
     5341 ttcttgagct gggtcgcata ggccatgcca gcgtacttcc aggcgacacg ctggaaccgc
     5401 gacgcgatgg tgacgaggat ctgcggtgtg tactgccgcg cgctcgagat ccacgcgtcg
     5461 tgcagcaacg catcgagctc gctcgatgcg ggcgcgaccc acgacagcgc atggcgcagc
     5521 ggatcatagt agcagagccc gcgctcgaga tcccggcatg cgtggacggc gaggtacagc
     5581 tcgagctcgt cgttaccttg ggactcatgg acacctccat catgacggga gagggatggg
     5641 aggtgtccac gaaaacgggg caggcctacg cggctcgtcg ccgcgaaaca cgcccgagta
     5701 gcgttcgagc gcctcgcaca gcgcgctcgt gcgtgcctgc gcctcggtgc gccccttgcc
     5761 cgagctgttg ttgcgcagcc catccttgag aaggaacagg tgatcgttct tgagcgcgaa
     5821 gttgtgcccg gccatgtata cgcgaagcgg gccggcgccg tgccatgccg aaggcgtcat
     5881 ctcgcggacg atgccggtga gcgggctgac gtggtgctgg tagcgttgga gcacagcctc
     5941 gggttcgtcg cgtcgcatcc cgctggtcga ctcgccggcc acacgctgag ccgtgagctg
     6001 gaccggcagg ccggcgcgcg tgccaagccg cgggttgcca cagtctggac actgcggccg
     6061 gcgcacgatc ggatgtcgct cgaacgtcag cgaagtcagg tcggtcacga gcacgtgcga
     6121 gccgagcccc gggtttgcgc cggtggtcag gaagcgcacg agctgcgtga tcgcgaccga
     6181 cgacgcatgc cgctcgaaca gaggcacgcg cgcgcgaggc agtgcgagtg gcatccgctt
     6241 gcccgtacgc cgctcgatgt atgcctcgac ctctcggttc gcgcgcaggc ggtactcgag
     6301 acagcgccag cacgcggtct gccccgcgac gaccagcggt ccgatccacg cccgcacgcc
     6361 gaccggcttc acgaggaaca gcggcacacc gcgcgcgagg cacgcgcggt tgagctcgaa
     6421 gaggctcggc cgcaggtagt cgtcgacgat cgcgaggtag atcttcgcat gcatacccgg
     6481 cgacagcccg gacgccggca acggcgctgg ccagtggcag gcagaggcca gctgcgcagc
     6541 ggcagctcgc cacgtgctcg gctgatccac cacaactgca cctcggtcca gcgcgcgacc
     6601 gtcgccgcac tcgaggacct gttgatcccc ggtcgcgccc ctggccaagc atcggcgcag
     6661 tcggtcaggg ggtcgggtcc gcggcgcagc tcgactccgc ccagcggccg aggaagtggc
     6721 ccgaggtcgc ggcctggccc tcgaagtagg cgccgtgcgc ggtgccgtgg tcgccctggc
     6781 gatcgatcca gcgcgcctgg tactggccgt cggcgaacgt cccgccgagc aggccgcgga
     6841 agtggccctc gcggtcgatg aacttgccga acagcaccga gtcgccattg cggcgctggc
     6901 cgtagacacc gcgcacgtgg ccgaccggcg cgccgttctc gtcggtcacc acgccgagga
     6961 accggccgag gtgcgccgac agcgcgtgcc agcggccgcg catgaagccg cggtcgcacg
     7021 ccgcggcctg gcggtggacg ccgatcgcga ccatcttgaa gccgtcgccc gcgtcgacgg
     7081 cgatcgggcc ggccgcgagc tgcgagaggt cgaacgtgta cgtggtcgcg ccgtcggccg
     7141 aggtgtaggt cagcgtctgg ctgccggtcg cggtcgggct cggcggatcg agcacggtca
     7201 gcgcgaggcc gtcgacgtag ggccgggtca ccgactggaa gctgacggcg tcgagcgcgg
     7261 cgcgcggcag gagcttgtcg gtcctgtcct cgaacgcgat cgtgcgccgg acgatcagcc
     7321 cgccgcgcga gaccttgagc gagccggtcc agtcgcgcga caccgtcgcg tccttgtccg
     7381 cggggatctt gccccacacg agcatcatgc ggaacccggc ggtggccgag gcggtgtcga
     7441 tcgccgtggt ggccgggtcg ccggcgagcg tgtcggtggc ggcgctgtcg ctctcgatcg
     7501 cggcggcggt gaagtcggtc tgggcgccga actcgggggc ctcgtcggag gtgtcgaagc
     7561 cgccgttggg ctgctccagg gccgacgaca cgtcctgact gtcgctgccg ccgccgctgt
     7621 cgaggcaagc ggctgcgggg agggcgagca accaggcgag ggatagggaa ccgtgacgca
     7681 tgacaaatct ccttggcaac gcgggatctc tggtcgcgtt gcagagcaag ccgccggcca
     7741 gccgataacc gcgcgggccg cctgacgaac ccgggcggac tggaacgccg agataccggc
     7801 ggggtcagcg gccgccgatc aagcggtacc tcgaggtgcc ggtgtttacg gctcgggcca
     7861 ccgccgcggg cgttgccgat ctgggcgcgc atggcccgcg gtcgacttcg cgccgcgcaa
     7921 cttctatggc cccaggccct ggaaggacag cgtccgtacc tcgctcgatc gccacttcgg
     7981 tgcggggccc cggcttccca cggtcacgtc cggtgcgatc gcttccagcg atcggctcat
     8041 caaggaggtc gagatcctgg acctctggcg gaagcaggcg cgccagatcc gcgcggtcga
     8101 gatggagtcc gccggcctct acaaggcggc ccatggacgc gtcccgttcc tcgcgatccg
     8161 cgggatcagc gacatcgtcg ggttcaagcg cgatcccggc tggacgtcct acgcgtgcca
     8221 taccgccgcg gcgttcaccc gcgcgttctt gcgggcggcc cgctggcgct gaccatagct
     8281 gtcacttctt cgcggccagc ttggccttca gcttctcgat ctcggcgtca cgcgccgggt
     8341 gatccggttg cagcggcacg gcgatgttct cgatcttgcc gaggtagtac gccgcgaagt
     8401 ccttgacgcg gagcttcagc ttgttgccgg tcatcgcctc gcggctgccc tcgaagaaga
     8461 tccagcgtgc tggctccgga cgcggtcgtg ccgctgttcc acagctcggc cgacttcatg
     8521 aaggtcgagc ttggcacgcc ctgcgagatc accttgagcg gcagcttggt gaacgcaccg
     8581 ccactgtcgg tccgccagcc ccactcgacc gagccataat acgaatcctt ctggttgccc
     8641 ttgatggcca gcgcggtggt ctcgtagatc tgcgccgaat cggtggcgcc gttgttcagg
     8701 ccggtctcgt cgttgagtag cgcgtcctgc tccttcaatg cacccgaagc atcggtgtac
     8761 cgaaacccgt gtcggcccca gtcgttcttc ttcgtgacgt cgggatcggg cgttgtctgg
     8821 tcgagtcgcg tatcgcccgc cggcgcgccc tcggcggcat acagcgggtt gcgattgtaa
     8881 tccgcctgat cgatgtgcgc gccctcgtcg gtctcgccgg tcgcggcgtt caccgtcctg
     8941 gcatcgcccg actggatgct atgcgcgcgc gtcgtcgcat tcgcgttgat gtagtacggg
     9001 ctgcccgcct tgatcgtctt cgcggtctgc gtcgatgccg atgagctcgg catcgacgtt
     9061 gggcttgggc ttgaaccgca gatcgatctt gatgccgcgg cggtcgccgg atcgattgat
     9121 cgtgtagttg tcgtcgaacc actcgccata gggggtgtcg accttgcgct gcaccgcgcc
     9181 gccaccggcg tgcgatccgc cgccaccggc gtaggcgtcg agcagactct cggcggactt
     9241 gccctggacc accagatcgg cgacccggtc ggcgtgctgc tcgtgcggat cgccgtcgcg
     9301 cccgacgccg ccagcgaggt gaacgcccgc gcgctgctgg acgacgtggg ccgcttcgtg
     9361 cgccgcgagg tgcagtgacg gagagtcccg gaacgccacc tggttgccgg tggcagatgc
     9421 tcgcgaagtt gcccgcgacg cccgtggcga cccgcgctgg cacgccgaac gcgctgctcg
     9481 tgttcgagcg cgtcgcgacc atgatcggcc cgccatcgcc cttcgagtag tacagctcga
     9541 ggccgtcctc ggacacttca gggtaacgcg cctcctcgcc ggagacgttg acgttgagca
     9601 gcgcggtcgc cacgccgaac ccgtcggccg cgctcgcgcg gctcgtggag tagacgtcga
     9661 acacgtcggt gcccgatagc tgagcgtcga agtacagcgt cagcccatcc ccggatggta
     9721 cgggcacgcg gatctgaccg gtgtaattga cggcgacctg ggtgaagggc gaccaggtca
     9781 gcgccgcgtc aggccccggg gcatcgccgg tgtccgccgc cgcgtcggct ggaagctttg
     9841 ccgcgacgct accgcagccg cccgcgccca gcgcatgcac cgcgatcacg gcgccgacga
     9901 caccgcggac cacgtcgtgg ctatcgagct ttcgcacgga ccgcgatgat agcagaccgc
     9961 gccgccgggc cgggtaccgc gccacagcgc gcttgggaag cgcgggcgga ccggaccgcc
    10021 gaggttcccg gccgggcgtg gagccgccga tcgagcggta cctcgaggtg ctggtgttac
    10081 ggctcggtca ccgtgacggt ctcgccggcc tcgaccttca cgctgcgccg ggccacgacg
    10141 tcgttgctgc gggggtcgac cagctcgacg acgtgcgagc cggtggcgag ccggcggttg
    10201 aacagcggcg tcggaccgac catctggccg tcgatcagca cgttgagata gcggtcgccc
    10261 ttgtgcttgg cagtgatccc accgaaggtc gcggcggcgt cggccggtgc ggctgtgccc
    10321 gcggcctcgg cgtc
//
LOCUS       DASVOR010002254         5430 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113074,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002254 DASVOR010000000
VERSION     DASVOR010002254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113074"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VF516_41735"
     CDS             <1..767
                     /locus_tag="VF516_41735"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEX8114346.1"
                     /translation="YLGGLAGLLAVISGLTAASFAADARNSKLAERMARADQRAQLAR
                     KLARDNGVPATGGADVFMTAPMARARALYATRCKNCHDADSMERKGPVIAPGHGGRDW
                     IRAFVKNPSGEPFWGLTKLAKTDAAMKSFSQMPQVDLDGIVELIYSESGATDIDADRK
                     KHGVEVFEIACSDCHAREDGMPGSSAPGLAGVGTRAYYRSFISNPKSMFHMGKDKSQM
                     PRFDKELSLTDLDALAEYLVWLRTATRDDLARLDPY"
     gene            815..2464
                     /locus_tag="VF516_41740"
     CDS             815..2464
                     /locus_tag="VF516_41740"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEX8114347.1"
                     /translation="MPTFAFQDILEAGHHDATPYRKLTGDLVQPLTAGGRRFLEVAPG
                     ALTLLTKTAMRDIAHLLRPGHLAQLRAILDDKDASPNDHFVALELLKNANIAAGGVLP
                     GCQDTGTAIVMGKRGQLVLTAGNDEAAIARGVFDTYAEANLRYSQVAPLDMFEENNTG
                     NNLPAQIELYAVDGDEYHFLFMAKGGGSANKSLLFQETKALLNPASLTAFLDQKLRSL
                     GTAACPPYHLAVVIGGTSAEYALKTAKLASARYLDDLPTSGNALGRAFRDLEWEAKVV
                     DIARTTGIGAQFGGKYFCHDARVIRLPRHGASCPVAIAVSCSADRQALGKITRDGVFL
                     EELERDPAKYLPEVAPGDIAGDEIRLDLHRPMAEIRQTLSRYPIKTRLSLTGPMIVAR
                     DIAHAKLKERVDRGEGLPQYFKDHCVYYAGPAKTPAGYASGSFGPTTAGRMDSYVDLF
                     QSHGGSMVMLAKGNRSPQVTSACKTHGGFYLGSIGGPAARLAKDCIKHVEVLEYPELG
                     MEAVWKIDVVDFPAFIVVDDKGNDFFAGITGGSGPVALKRS"
     gene            complement(2474..2758)
                     /locus_tag="VF516_41745"
     CDS             complement(2474..2758)
                     /locus_tag="VF516_41745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114348.1"
                     /translation="MTYVLDGTPEQAMAAFAEHLRRAHAGGPDAEHEAQMAMVSLVRL
                     SLIHLDDVAELDEELRRLGQHDEQGPVDARAIPRGLPASHWWWRYPDPFK"
     gene            complement(2755..2946)
                     /locus_tag="VF516_41750"
     CDS             complement(2755..2946)
                     /locus_tag="VF516_41750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114349.1"
                     /translation="MQLALASCDQSICGVIIQKTYPSPSGGEPQVMEAIVYVDPSGKV
                     TLASYGAAKAMPPGKPGAP"
     gene            3360..3557
                     /locus_tag="VF516_41755"
     CDS             3360..3557
                     /locus_tag="VF516_41755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114350.1"
                     /translation="MAAGARRKAACELLGTNPVGNPPDTMHFYSPISMGKKQPAWAKD
                     SAEVHVDGISKERFRFFHGVK"
     gene            3936..4340
                     /locus_tag="VF516_41760"
     CDS             3936..4340
                     /locus_tag="VF516_41760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114351.1"
                     /translation="MTFAEAERAPVGIDATTTLLTYMFRVIDPLYHDQLRAKIRKLGP
                     HAEETAMTIAEQWHEEGRKEGIAKGREEGIAKGREEGRIAALRSQLRAKFGAQALDAT
                     YEARLQAATPAVVDHYLQRVLVADSLAAVFED"
     gene            complement(4375..4821)
                     /locus_tag="VF516_41765"
     CDS             complement(4375..4821)
                     /locus_tag="VF516_41765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114352.1"
                     /translation="MSDAPIVITGHAYGRRATLELAGDRLMWRAQRGARRIAENIATT
                     IHDVRGARWLDLAWSRGGTALIGIGAMWAVTESLAAGLTAGAVGLAMLIWRRARPRHF
                     LVLDVGDRRLVLRVTGGSAPSARALAGRIQRALASGELPSTPPTLP"
     gene            4976..>5430
                     /locus_tag="VF516_41770"
     CDS             4976..>5430
                     /locus_tag="VF516_41770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114353.1"
                     /translation="MGSPGFVGIDFGTTNSAIAIADDRGDVRLVPLAGAPHWRTVLYF
                     EPGGGLTAGAPAIARYLETEGEGRLIQSIKSHLASAAFSRTAIFGKRWQIDDMIAAYL
                     RQVRAAAPVDLGRRAVVGRPVRYWGAESPDDDARAVARMQDALAKAGFD"
BASE COUNT          881 a         1910 c         1902 g          737 t
ORIGIN      
        1 cgtacctcgg gggcctcgcc gggctgctcg cggtgatctc cgggctcacc gcggcgtcgt
       61 tcgcggcgga cgcgcgcaac agcaagctcg ccgagcgcat ggccagggcc gaccagcgcg
      121 cccagctcgc gcgcaagctc gcccgcgaca acggtgtgcc ggcgaccggc ggcgccgacg
      181 tgttcatgac cgcgccgatg gcccgcgccc gcgcgctgta cgcgacccgg tgcaagaact
      241 gccacgacgc cgacagcatg gagcgcaagg gcccggtgat cgcgcccggc cacggcggac
      301 gcgactggat ccgggcgttc gtcaagaacc ccagcggcga gccgttctgg ggcctgacca
      361 agctggccaa gaccgacgcc gcgatgaagt cgttctccca gatgccgcag gtcgacctcg
      421 acggcatcgt cgagctgatc tacagcgagt cgggcgccac cgacatcgac gccgaccgca
      481 agaagcacgg cgtcgaggtg ttcgagatcg cgtgcagcga ctgccatgcc cgcgaggacg
      541 gcatgcccgg ctcgtcggcg cccggcctgg ccggcgtcgg cacccgcgcg tactaccgca
      601 gcttcatctc caaccccaag tcgatgttcc acatgggcaa ggacaagagc cagatgccgc
      661 ggttcgacaa ggagctcagc ctgaccgacc tcgacgcgct cgccgagtac ctggtgtggc
      721 tgcgcaccgc gacccgggac gacctcgcca ggctcgatcc gtactagccg cgcgtccggc
      781 gatccgtacc aggatccgta ctaggctccc cgccatgccg acgttcgcgt tccaggacat
      841 cctcgaggcg ggccaccacg acgccacgcc gtaccgcaag ctcaccggcg acctcgtcca
      901 gccgctcacc gccggcggcc gcaggttcct cgaggtcgcg cccggcgcgc tgaccctgct
      961 gaccaagacg gcgatgcgcg acatcgcgca cctgctccgg cccggccacc tcgcgcagct
     1021 gcgcgcgatc ctcgacgaca aggacgcctc gcccaacgac cacttcgtcg cgctcgagct
     1081 gctcaagaac gccaacatcg ccgccggcgg cgtgctgccc ggctgccagg acaccggcac
     1141 cgcgatcgtc atgggcaagc gcggccagct cgtgctgacc gccggcaacg acgaggccgc
     1201 gatcgcgcgc ggcgtgttcg acacctacgc cgaggccaac ctgcgctact cgcaggtcgc
     1261 gccgctcgac atgttcgagg agaacaacac cggcaacaac ctgccggcgc agatcgagct
     1321 gtacgcggtc gacggcgacg agtaccactt cctgttcatg gccaagggcg gcggctcggc
     1381 caacaagagc ctcttgttcc aggagaccaa ggccctgctc aacccggcga gcctgaccgc
     1441 gttcctcgac cagaagctgc gctcgctcgg caccgcggcg tgtccgccgt accacctcgc
     1501 ggtcgtgatc ggcgggacca gcgccgagta cgcgctcaag accgccaagc tggcctcggc
     1561 gcgctacctc gacgacctgc cgacctccgg caacgcgctc ggccgcgcgt tccgcgacct
     1621 cgagtgggag gccaaggtcg tcgacatcgc gcgcaccacc gggatcggcg cgcagttcgg
     1681 cggcaagtac ttctgccacg acgcccgcgt gatccgcctg ccgcgccacg gcgcgagctg
     1741 cccggtcgcg atcgccgtat cgtgctcggc cgaccgccag gcgctcggca agatcacgcg
     1801 cgacggcgtg ttcctcgagg agctcgagcg cgatcccgcg aagtacctgc ccgaggtcgc
     1861 gcccggggac atcgccggcg acgagatccg gctcgacctc caccgcccga tggccgagat
     1921 ccgccagacc ctgtcgcgct acccgatcaa gacccggctg tcgctgaccg gcccgatgat
     1981 cgtcgcgcgc gacatcgcgc acgccaagct caaggagcgg gtcgaccgcg gcgagggctt
     2041 gccgcagtac ttcaaggacc actgcgtcta ctacgccggg ccggccaaga ccccggcggg
     2101 ctacgcctcc ggctcgttcg ggccgaccac cgccggccgg atggacagct acgtcgacct
     2161 gttccagtcc cacggcggca gcatggtgat gctcgccaag ggcaaccgct cgccgcaggt
     2221 cacctccgcc tgcaagaccc acggcgggtt ctacctgggc tcgatcggcg gccccgcggc
     2281 ccggctcgcc aaggactgca tcaagcacgt cgaggtcctc gagtaccccg agctcggcat
     2341 ggaggcggtg tggaagatcg atgtcgtcga cttcccggcg ttcatcgtgg tcgacgacaa
     2401 gggcaacgac ttcttcgccg ggatcactgg aggcagtgga ccggtggcgc tcaagcgcag
     2461 ctgacccgcg cggctacttg aacgggtccg ggtagcgcca ccaccagtgg ctcgcgggca
     2521 acccgcgcgg gatcgcgcgc gcgtcgaccg ggccctgctc gtcgtgctgg ccgaggcggc
     2581 gcagctcctc gtcgagctcc gcgacgtcgt cgaggtggat cagcgacagt cgcacgagcg
     2641 agaccatcgc catctgcgcc tcgtgctcgg cgtcgggccc gccggcgtgc gcgcgccgca
     2701 ggtgctcggc gaacgccgcc atcgcctgct cgggggtgcc gtcgaggacg taggtcatgg
     2761 cgcgcccggc tttccgggag gcatcgcctt ggcggccccg tagctcgcga gcgtcacctt
     2821 gccgctcgga tcgacgtaca cgatcgcctc catgacctgc ggctcgccgc ccgacggtga
     2881 cgggtaggtc ttctggatga tgacaccgca tatagattga tcgcaacttg cgagtgcgag
     2941 ctgcatggcc gcagtcccgc gatcgggagt ggcatcgatt ttcgctccgg ttgaactcgc
     3001 ggcattcagc ggcagcgacg gtggacccgc tgagctcgcc atccgatgcg cggccgcgct
     3061 ggcgcatcgt gcggcatcga gcggcggaac cgactgcatg gtgacgagat ccccatgtct
     3121 cagggaagtt gtcgcctgag ccggcatgac tgaccgacgt cagacccaac cgaggcggcg
     3181 aacatcgaga agctgaagat ccggaacctg gagcgggagc tcaagcggaa ggacaaggcg
     3241 ctggccgagg cggcggcgct gctggcactg aaaaaaaaag cccaggcgat ctggggggac
     3301 gaggacgacg acacgacgcc gccgagcgac gactgatccg cgggctgatc gacgaggcgg
     3361 tggccgccgg tgcgcggcgc aaggcagcgt gcgagctctt ggggaccaac ccggtcggca
     3421 atccgccaga cacgatgcac ttctacagcc cgatctcgat gggcaaaaaa cagccagcct
     3481 gggcgaaaga ctccgcggaa gtccatgtgg acggcatctc gaaggagcga ttccgcttct
     3541 tccacggcgt gaagtgagcg ttcaacgctc gccgggtggt cgcgcggggg cgggcttact
     3601 tcgcgagcaa ctcggcacgc gcggtagcgt acagctcgag gagcagcgcc agcagcgtgc
     3661 gcaggtcgtc gatcttcggt gtcggcgtca ggctgtaccg gaacgtggtc gcgtacgccg
     3721 acagccgctc gagcggtcga aaccggcgcg ccaggggtct cccggcggta gcggacgagg
     3781 ctcgctgccg ggtcggccat cgatgaggat caccagatcg tggtccttgg tgttgagcag
     3841 gccgcggtcg aggcggacgg cgctcaggat cttttctgcg gcctgctgca gatgatttgc
     3901 cgccaggcct gctggacagc ttcgatacct ggatcgtgac ctttgccgag gcggaacgag
     3961 cgccggtcgg tatcgatgca accaccaccc tgttgacgta catgtttcga gtgatagatc
     4021 cgctgtacca cgaccagctc cgtgccaaga tccgcaagct tggcccccac gccgaggaaa
     4081 ccgccatgac catcgccgaa cagtggcacg aggaaggccg caaggaaggc atcgcgaagg
     4141 gccgcgagga aggcatcgcg aagggccgcg aggaaggacg gatcgccgcg ttgcgctccc
     4201 agctccgcgc aaagttcggg gcgcaggcgc tggacgcgac gtacgaagcc cgcctgcagg
     4261 cagcgacgcc cgcggtggtc gaccattatc tccagcgcgt gctggtcgct gattcgctcg
     4321 ccgcggtgtt cgaggactga ccaccgccca tcgcaggggc tgactccgca gcgctcaagg
     4381 cagggtgggc ggggtgctcg gcagctcgcc gctggccagc gcgcgctgga tccggccggc
     4441 gagggcgcgg gcggacggcg ccgagccgcc ggtgacgcgc aggaccaggc ggcggtcgcc
     4501 gacgtcgagc acgaggaagt ggcgcgggcg agcacggcgc cagatcagca tcgcgaggcc
     4561 gaccgcgccg gcagtgagcc cggcggcgag gctctcggtg accgcccaca tcgcgccgat
     4621 gccgatcagc gcggtgccgc cgcggctcca cgcgaggtcg agccagcgcg cgccgcgcac
     4681 gtcgtggatg gtggtcgcga tgttctcggc gatccggcgc gcgccgcgct gggcccgcca
     4741 catcaggcga tcgccggcga gctcgagcgt cgcgcgccgc ccgtaggcgt ggcccgtgat
     4801 cacgatcggc gcgtccgaca cggcggcgag cttagcgcag ctgggcccgg cactcgcgct
     4861 gccacctggc aggggcgccg gcgccgcggg ggcacgagtc atcccgattg gctcctacag
     4921 gatagggagg aggcggtagc tagtaaagcg aaatcggatc gccgacaacg gcgtcatggg
     4981 cagtcctggt ttcgtgggca tcgactttgg aacgaccaac tcggcgatcg cgatcgccga
     5041 cgaccgcggc gacgtacggc tggtgccgct ggcgggcgcg ccgcactggc gcaccgtgct
     5101 gtacttcgag ccggggggcg ggttgaccgc gggcgcaccg gcgatcgcgc gctacctgga
     5161 gaccgagggc gagggccggc tgatccagtc gatcaagtcg cacctggcga gcgcggcgtt
     5221 ctcgcggacg gcgatcttcg gcaaacgctg gcagatcgac gacatgatcg cggcgtacct
     5281 gcgccaggtc cgggcggcgg cgccggtcga cctcggccgc cgcgcggtgg tcggccggcc
     5341 ggtgcgctac tggggcgccg agtcaccgga cgacgacgcg cgcgcggtgg cgcggatgca
     5401 ggacgcgctg gccaaggccg ggttcgacga
//
LOCUS       DASVOR010002255         3803 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_286281,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002255 DASVOR010000000
VERSION     DASVOR010002255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286281"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..632)
                     /locus_tag="VF516_41775"
     CDS             complement(204..632)
                     /locus_tag="VF516_41775"
                     /inference="COORDINATES: protein motif:HMM:NF027967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HEX8114354.1"
                     /translation="MTSRAVLAIATLAAIHGVTARATAEPPKPSGLRSRPVQLIGRPV
                     LESATPDSAVIRWTDTTGGGTDTHYGIVRYGLDPRHLDRTTRSPNRARNNATTMTYRV
                     WIDGLAPSTTYYYTVDAARADGNALGLHSPVSTFTTRPRS"
     gene            complement(644..2260)
                     /locus_tag="VF516_41780"
     CDS             complement(644..2260)
                     /locus_tag="VF516_41780"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HEX8114355.1"
                     /translation="MAWWLWGLWLVSLAGACAETDSHSTSDSDSATLAHTPPPYDPYP
                     PGLLPRDLQSEIARVNAEVDRIFREALAEWKAIPAPTLAGNPPTLANTGKRLVEILGK
                     LELFDKTISVEGNVACSFCHMPYTGFSGPISSVNATVVAYPGSYHFRFGKRKPQSYTY
                     SPFYPKLEFNQTQQDFYGGNFWDLRATGYKIESADAEQAQHPVVDALEHGFPDTACVV
                     FWLSRAPYRPLFELIWGKQAFDIDFPSSTRRICSIPGGAAVFHGNPTPVPLSRVDRGR
                     SNATFDQFALSITAYEASPDVSAFSSKFDAFLAGTAQLTPDELAGWQLFRGKAVCNTC
                     HLDGTEHSTAPITQASIASKAPLFTDFTSANLGLPRNPKNPFYFENVPDFFGFTPNPT
                     GAAFTDFGVGLFLRSEAGGDNVEAAWTRLAPMFNGKMQVSTARNADLRPCPSFVKAYM
                     HNGYLKSLKEVVHFYNTRDTYAAPSCTPETEKKTCWPPPEVSANKDMTIGHLGLTDAE
                     EDQLVAFLKTLSDGYTRPYTDFNTFTGKCP"
     gene            complement(2522..3118)
                     /locus_tag="VF516_41785"
     CDS             complement(2522..3118)
                     /locus_tag="VF516_41785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114356.1"
                     /translation="MRSLLFALVLAACVSTPPPVAPSPPEPPPAPPPAPMVTALPSMV
                     PPGQSAMPETAPDTPEAAPTAIAAQVAGPRRGGLEHAMANCPTAVQGAVTRAVNTEFG
                     VDLTITADDPVSQRRIVELAARHEQMGDPDGAIPPHTGRHGGPGGIGHCPVFHDSTTV
                     TFTKLRRGAVIHLRALLPSDVTRVQAIVADRLATFAQR"
     gene            complement(3229..>3803)
                     /locus_tag="VF516_41790"
     CDS             complement(3229..>3803)
                     /locus_tag="VF516_41790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114357.1"
                     /translation="AALSIVPGGVFACAGLAYLAHERFSEARRRARSRDLLLALGDLE
                     VALHVADGPEAAKGIAEQLAPYTRDAPITRPEVYEDAKRRMRAQAEGKGEVSARRELE
                     HALLVGADVVYLRGDYLQVGQVAFRISEVREYALRGANLPLTGGRLLQAAMGLLVVAA
                     EERAATGEDVGLLSKRIADYEAWSGHTAGR"
BASE COUNT          520 a         1260 c         1440 g          583 t
ORIGIN      
        1 gctgtcctgg cagctcgccc gtgcctacgc ggcaaccggc gacgccgcgc acgcgcgacc
       61 gctgctcgag gcgatcgtcc aggcgcacgg ggcgcgcagc ggcgacgcca gcgcgctgct
      121 cgcgcagctg gagcgctgag cgccgatcga ctcggtcgcg gtggtcgctg gtccaggccg
      181 ccgccgggca atcccggatc ggatcacgac cgtggccgcg tcgtgaacgt gctcaccggg
      241 ctgtgcaggc ccagcgcgtt gccgtcggcc cgtgcggcgt cgaccgtgta gtagtaggtc
      301 gtgctcggcg cgagcccgtc gatccacacc cggtatgtca tcgtcgtcgc gttgttccgg
      361 gcccggttcg gcgacctggt tgtgcgatcc aggtgcctgg ggtcgaggcc gtatcgaacg
      421 atcccgtagt gcgtgtcggt gcctccgccc gtggtgtcgg tccaccggat cacggcggag
      481 tccggcgtgg cggactcgag caccggccgg ccgatgagct ggaccggccg ggagcgcaac
      541 cccgacggct tcgggggctc ggcggtcgcc cgcgcggtga cgccgtggat cgcggcgagc
      601 gtcgcgatgg cgaggacggc acgactcgtc atgacgcgtc gacctagggg catttcccgg
      661 tgaaggtgtt gaagtcggta tacggtcgcg tgtagccatc gctgagcgtc ttcaggaacg
      721 cgacgagctg gtcctcttct gcgtcggtca ggccgaggtg gccgatcgtc atgtccttgt
      781 tcgcgctgac ctcgggaggc ggccagcacg tcttcttctc ggtctccggg gtgcagctgg
      841 gcgcggcgta ggtgtcgcgc gtgttgtaga agtggacgac ctccttcagg ctcttcagat
      901 agccattgtg catgtaggcc ttcacgaagc tcgggcacgg ccgcaggtcg gcgttgcgcg
      961 ccgtcgatac ctgcatcttg ccgttgaaca tcggcgcgag cctggtccag gccgcctcga
     1021 cattgtcgcc gccggcctcg ctgcgcagga acaggccgac gccgaagtcg gtgaacgcgg
     1081 cgccggtcgg gttcggcgtg aacccgaaga agtcggggac gttctcgaag tagaacgggt
     1141 tcttggggtt gcgaggcagc ccgaggttgg ccgacgtgaa gtcggtgaac aggggcgcct
     1201 tgctcgcgat gctggcctgc gtgatcggcg cggtgctgtg ctcggtgccg tcgaggtgac
     1261 aggtgttgca caccgccttg ccgcggaaca gctgccagcc ggccagctcg tccggggtca
     1321 gctgggcggt gccggcgagg aaggcatcga acttcgacga gaacgcgctg acgtccggcg
     1381 acgcctcgta cgcggtgatc gacagcgcga attgatcgaa cgtcgcgttg gaccgcccgc
     1441 ggtcgacgcg gctcagcggc accggcgtcg ggttcccgtg gaacacggcg gccccgccgg
     1501 gtatgctgca gatccgccgg gtgctgctcg ggaagtcgat gtcgaacgcc tgcttgcccc
     1561 agatcagctc gaacagcggc cggtacgggg cccgggacag ccagaacacg acgcacgcgg
     1621 tgtccgggaa gccgtgctcc agggcatcga cgaccgggtg ttgcgcctgc tccgcgtcgg
     1681 cgctctcgat cttgtatccg gtggcgcgca gatcccagaa gttgccgccg tagaagtcct
     1741 gttgcgtctg gttgaactcg agcttgggat agaatggtga atacgtgtag gactgtggct
     1801 ttcgcttgcc gaaccggaaa tggtaggagc cgggatatgc gaccacggtc gcattcaccg
     1861 acgagatcgg cccgctgaaa ccggtatatg gcatgtggca gaacgagcag gcgacattgc
     1921 cctcgaccga gatcgtcttg tcgaacagct cgagcttgcc gaggatctcg accagccgct
     1981 tgccggtgtt tgccagcgtc ggcgggttgc cggccagcgt cggcgccggg atcgccttcc
     2041 actcggcgag cgcctcgcgg aagatccgat cgacctcggc gttgacgcga gcgatctcgg
     2101 actgcagatc gcgcggaagt agacccggcg gataggggtc atagggtggt ggagtatggg
     2161 cgagggtggc actgtcgctg tcgctggtgc tgtgactgtc ggtttcagcg cacgcgcccg
     2221 ccagcgagac gagccacaac ccccacagcc accatgccat tcgtgcggac cgcattccgg
     2281 gacgccgtgc acctcgcgtt ccgcggctgg cccatgaaga tctctcgcat ctgccgtgcg
     2341 cagatgcccg catgcggcga cgaggcgcgt gcggcggagc gacgctcggg gcgccgacgg
     2401 accgccgccc gccgaccgga acgtcacggt gcgccccgcg gcggcgtgtc cagcatcgcg
     2461 gcgagctcgt cgggaacctg ctccggtgtg gcgggatgta cgcggccgcg ctgcgacggg
     2521 ctcagcgctg ggcgaacgtg gcgagccggt cggcgacgat cgcctggacg cgggtgacgt
     2581 cgctcggcag cagggcgcgc aggtggatca cggcgccgcg gcgcagcttg gtgaacgtca
     2641 cggtggtcga gtcgtggaac accgggcagt ggccgatccc gcccgggccg ccgtgtcgcc
     2701 cggtgtgcgg cggtatggcg ccgtcggggt cgcccatctg ctcgtgccgg gcggcgagct
     2761 cgacgatgcg gcgctggctc accggatcgt cggcggtgat cgtgagatcg acgccgaact
     2821 cggtgttgac ggcgcgcgtg accgcgccct ggaccgcggt cgggcagttg gccatcgcgt
     2881 gctcgagccc gcctcgccgc gggccggcga cctgcgcggc gatcgcggtc ggcgcggcct
     2941 cgggcgtgtc cggggcggtc tccggcatcg ccgactgccc cggcgggacc atcgacggca
     3001 gcgccgtgac catcggggcc ggcggcggtg cgggcggtgg ctccggtggc gacggcgcga
     3061 ccggcggcgg ggtcgagaca caggcggcga gcacgagcgc gaagagcagc gagcgcatgc
     3121 ccggtgtgac tgcgaacgat gtaccagcgc agatcccgcc ggatcggcgc atcccgcgca
     3181 gcggctgcgc cccggggatc gcgcgacgaa ctggctgcgc gaggaacgtc agcgaccggc
     3241 ggtgtggccc gaccacgcct cgtagtcggc gatccgcttc gacagcagcc cgacgtcctc
     3301 gccggtcgcg gcgcgctcct cggcggcgac caccagcagg cccatcgccg cctggagcag
     3361 gcggccgccg gtgagcggca ggttggcgcc gcgcagcgcg tactcgcgga cctccgagat
     3421 ccggaacgcg acctggccga cctgcaggta gtcgccgcgc aggtagacca cgtcggcgcc
     3481 gaccagcaac gcgtgctcga gctcgcggcg cgccgagacc tcgcccttgc cctcggcctg
     3541 ggcgcgcatc cggcgcttgg cgtcctcgta gacctcgggc cgggtgatcg gagcgtcgcg
     3601 ggtgtacggc gcgagctgct cggcgatgcc cttggcggcc tcgggcccat cggcgacgtg
     3661 cagcgcgacc tcgagatcgc cgagcgcgag caggaggtcg cggctgcgcg cccggcggcg
     3721 ggcctcgctg aaccgctcgt gcgcgaggta ggcgaggccg gcgcacgcga acacgccgcc
     3781 cgggacgatc gagagcgcgg cgg
//
LOCUS       DASVOR010002256         2719 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_375143,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002256 DASVOR010000000
VERSION     DASVOR010002256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375143"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..640
                     /locus_tag="VF516_41795"
     CDS             <1..640
                     /locus_tag="VF516_41795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEX8114358.1"
                     /translation="TTAATTPVRKWQDAIRKARTRDRERPGHALRSLPTAAGRWSLLA
                     PTDVASVDAEASARQLLHRYGVVFRDLVLRESSLPPWRDLLIALRRLEARGEIRGGRF
                     VTGFVGEQFALPEALDELRAVRHPGPTPDVCRVAATDPVNLVGILSPGPRVPAIVGNM
                     VLYVDGHTVASLEAGKLVLRAPLPPGARVDDDLTYHPPPRPAAVATQATLPL"
     gene            complement(579..1316)
                     /locus_tag="VF516_41800"
     CDS             complement(579..1316)
                     /locus_tag="VF516_41800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114359.1"
                     /translation="MARDESVALASTAASTALASRTVGQSKEWTIDPPAGWQEDAATE
                     RQLRDQMTAQKKIKFQGIEIRKWSPAVEDGMLLVQWFVLPIDGNIERQIDAFDRGMVK
                     GFAQSATTPDGSRKVVGSMVIRDVRVDRLRGTDIQAHMVRRYQPASDGLHVLITVCDV
                     TSDPALCDAALDGAQFTVPDPISLDGWTDAQVYRLAEVFGMLFGIAAMLLVLYVLRRR
                     SRARTAAVKAAGSPGWPPRPGGAADGR"
     gene            1442..2290
                     /gene="nth"
                     /locus_tag="VF516_41805"
     CDS             1442..2290
                     /gene="nth"
                     /locus_tag="VF516_41805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEX8114360.1"
                     /translation="MHGSLLSTMPPDPDSDAAPAPSIAPVETTAAPLPRDRKARAARI
                     LEILDRYYPEVEMPLDHGDPFQLLVATILSAQCTDARVNMVTPALFGRAPDAPAMARL
                     TAQQIMPYIRTCGLAPGKAKALANMSRILVAQHGGEVPRDMAALEELPGVGHKTASVV
                     MAQAFQEPAFPVDTHIHRLAGRWQLSRARSVEEVERDLKAVFPRERWNTLHLQIIYFG
                     RQYCPARGHDPAACPICSWAMSKARAAAEARQGLSKPVRDATPARRAAAKSRARTGVS
                     RRARRA"
BASE COUNT          372 a         1094 c          886 g          367 t
ORIGIN      
        1 aaccaccgcc gccaccaccc cggtccgcaa gtggcaggat gcgatccgca aggcgcgcac
       61 ccgcgatcgc gagcggcccg gccacgcgct gcgctcgctg cccaccgccg ccggccggtg
      121 gtcgctgctg gctccgaccg acgtcgcgtc ggtcgacgcc gaggcctcgg cccgccagct
      181 cctgcaccgc tacggcgtcg tgttccgcga cctcgtgctc cgcgagtcca gcctcccgcc
      241 gtggcgcgat ctgctgatcg ccctgcgccg gctcgaggcc cgcggtgaga tccgcggcgg
      301 ccggttcgtc accggcttcg tcggcgagca gttcgccctg cccgaggcgc tcgacgagct
      361 ccgcgccgtc cgccaccccg gcccgacgcc cgacgtctgc cgcgtggccg cgaccgaccc
      421 ggtcaacctc gtcggcatcc tgtcgcctgg ccctcgcgtc cctgcgatcg tcggcaacat
      481 ggtgctctac gtcgacggcc acaccgtcgc gtcgctggag gccggcaagc tcgtgctgcg
      541 cgcgccgcta ccgcccggcg cccgcgtcga cgacgatctc acctaccatc cgccgccccg
      601 cccggccgcg gtggccaccc aggcgaccct gccgctttga ccgccgccgt acgcgcccgc
      661 gaccgcctgc ggagcacata cagcaccagc agcatcgccg cgatgccgaa cagcatcccg
      721 aatacttccg ccagccggta cacctgcgca tccgtccaac cgtcgagcga gatcggatcg
      781 ggcaccgtga actgcgcccc gtcgagcgcc gcgtcgcaca gcgcggggtc cgacgtcacg
      841 tcacaaaccg tgatcagcac gtggagcccg tcgctggccg gctggtagcg ccgcaccatg
      901 tgcgcctgga tgtccgtccc gcgcagccgg tcgacccgga cgtcgcggat caccatgctg
      961 ccgacgacct tccgcgatcc atccggcgtc gtcgccgact gggcaaaccc cttgaccatc
     1021 ccgcggtcga acgcgtcgat ctggcgctcg atgttgccgt cgatcggtag cacgaaccac
     1081 tgcacgagca gcatcccgtc ctcgaccgcg ggactccatt tccggatctc gatgccctgg
     1141 aatttgatct tcttctgtgc ggtcatctgg tcgcggagct ggcgctccgt tgcggcgtcc
     1201 tcctgccaac cggccggcgg atcgatcgtc cactccttcg actgcccgac tgtccgcgac
     1261 gccagcgcgg tcgacgctgc ggtcgacgcc agcgcgaccg actcatcgcg cgccacggta
     1321 gcagacccgc tccccgggga gccgggtcgc gccaggaacg tacgaggatc tcgccgcgcc
     1381 gcggcgggcc ggccgacgcg cccccggcga gccggcccgt tcgcactgga ctcacgccag
     1441 aatgcatggc tcgctgctgt cgaccatgcc cccggatcca gattccgacg ctgcgccagc
     1501 accgtccatc gcgccggtcg agaccaccgc tgccccgctg ccccgcgatc gcaaggcgcg
     1561 cgcggcgcgc atcctcgaga tcctcgatcg ctactacccc gaggtcgaga tgccgctcga
     1621 tcacggcgac ccgttccagc tcctggtcgc cacgatcctg tcggcgcagt gcaccgacgc
     1681 gcgggtcaac atggtcaccc cggcgctgtt cgggcgcgcg cccgatgccc cggcgatggc
     1741 caggctgacc gcgcagcaga tcatgccgta catccggacc tgcggcctgg cgccgggcaa
     1801 ggccaaggcg ctcgcgaaca tgagccgcat cctggtcgcc cagcacggcg gcgaggtccc
     1861 gcgcgacatg gccgcactcg aggagctgcc cggcgtcggc cacaagaccg ccagcgtcgt
     1921 gatggcgcag gcgttccagg agcccgcgtt cccggtcgat acccacatcc accggctcgc
     1981 cgggcgctgg cagctgtcgc gggcgcgcag cgtcgaggag gtcgagcgcg acctcaaggc
     2041 ggtgttcccg cgcgagcggt ggaacacgct gcacctgcag atcatctatt tcggccgcca
     2101 gtactgcccc gcgcgcggcc acgatccggc cgcctgcccg atctgcagct gggcgatgtc
     2161 gaaggcgcgc gccgccgccg aggcccgcca gggcctgagc aagccggtgc gagacgccac
     2221 gccggcgcgc cgcgccgccg cgaaatcgag agcaagaact ggcgtgtcac gccgcgctcg
     2281 acgggcctag cctggccgca gctgccagcg cgcgctccgt gatagcggcg tagcacggtg
     2341 cacacggcgc cggagatcgg ttgtgcacga caccgcgctt gcagggctgg actggccagc
     2401 actggagggt cgacgccccg gtcgccgtgc cagccgtcac aaatcatgag accagcttgc
     2461 gctgttacga ccgcggtcac cacccgcgcg cgcaagaccg tgatgcgctg cctcgacctc
     2521 gccgccggca ccgtgcggtg gaccgccgcg ctcgccggcg cgcgcgactg cacccagccg
     2581 ccggccgagc tccccggcgc gtacctcctg ccatgcccgg gtggaccgcg atcgtcgacg
     2641 agcgcaccgg cgcggtcagc gtcgacgccg gcggcgtcgg cctggtccag gaccggccgc
     2701 cctacctcct gcgccccgg
//
LOCUS       DASVOR010002257         2374 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_403449,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002257 DASVOR010000000
VERSION     DASVOR010002257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403449"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VF516_41810"
     CDS             <1..749
                     /locus_tag="VF516_41810"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8114361.1"
                     /translation="TLFRSARAAHGDAPPAEEPGPDAAARTGEDFRIGLRAVFGHPVV
                     RPIILTLMMWSVTGGFFIALYTPFCLRTIGLSERAFGVIIAMGGVGSLAGAMLARGLM
                     RRLGVGRTLVVTSALSLTCTLFIPIASTCASHAAALGFLAAHQLLGDGFSVAFVVVAV
                     TVRQTSLPREVLGRANAAVHVATTGMLVIASLVAGGLASLIGIRAAVWTGMLIGLSAP
                     LLLWPVRRVRDMSPGDLGSSAALPAADPHA"
     gene            853..1191
                     /locus_tag="VF516_41815"
     CDS             853..1191
                     /locus_tag="VF516_41815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114362.1"
                     /translation="MPKRSIPLLIVLNLFFPLIYHFYWLYKTKHEMTDRGADIPTTLL
                     AFIPFVHYYFLWKWSVGVEQVTRGKMSSAIAFVLMFFIGWIIGPAIIQATLNEVPEEG
                     VNLPQARVVA"
     gene            1314..>2374
                     /locus_tag="VF516_41820"
     CDS             1314..>2374
                     /locus_tag="VF516_41820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114363.1"
                     /translation="MDRPPPSAGGRRERVVHGLIVAAVAAVLSLASTAPYGILNQATY
                     LLDPLHRAFPELFHRDWLITEAPPYLPSFGWLARWLYAADPDGAVAMLVANFAVTFAT
                     YVALDWLVTAVCPSRRGFVLVASFVTVTMGRALGGSYLLAGYLQPSAPATLGWIVAMA
                     ALVRGRYRTCGVALAVAGAMHINFLVLGIGLFTLGAIARRGARLVDLAGLLVPQLVVL
                     AWLLPGLVASAGPGDDALRILVQFHAPGHYLGRRLISWVPGLVGWQLAGFAALPPAGA
                     RSPALVALWRFSLASCVIAVAAAFVVHVPAFEWLTQAFWPRIAPFGQLGCQVLIAAAL
                     VRQGTAPVSVARRAWLAGA"
BASE COUNT          240 a          854 c          868 g          412 t
ORIGIN      
        1 cgacgctctt ccgatctgcg cgggccgcgc acggcgatgc gccgcccgcc gaggagcccg
       61 gccccgacgc ggcggcgcgc accggcgagg acttccggat cggcctgcgc gccgtgttcg
      121 gccacccggt ggtccgcccg atcatcctga ccctgatgat gtggtcggtg accggcgggt
      181 tcttcatcgc gctgtacacg ccgttctgcc tgcgcacgat cgggctgtcg gagcgggcgt
      241 tcggtgtgat catcgcgatg ggcggggtcg gctcgctcgc cggcgcgatg ctcgcgcgcg
      301 gcctgatgcg gcggctcggg gtcggccgca cgctggtggt gacctcggcg ctgagcctga
      361 cctgcacgct gttcatcccg atcgcgtcga cctgtgcatc gcacgcggcc gcgctcggct
      421 ttctcgccgc gcaccagctg ctcggcgacg ggttctcggt cgcgttcgtc gtcgtcgcgg
      481 tcaccgtgcg ccagacctcg ctgccgcgcg aggtgctcgg ccgcgccaac gccgcggttc
      541 acgtcgcgac caccggcatg ctcgtgatcg cgtcgctggt cgccggcggg ctcgcgtcgc
      601 tgatcgggat ccgcgccgcg gtgtggaccg ggatgttgat cgggctgtcg gcgccgctgc
      661 tgctgtggcc ggtgcgacgt gtccgcgaca tgtcgccggg cgacctcggc agcagcgcgg
      721 cgctcccggc tgcggatccg cacgcgtgac tcccgcggat cgcgggctgg caaccgatcg
      781 ccactttgct gccggcgagt ccgtggctac gataggtcac acggcacggg gccgaggtgc
      841 ccgggagaca tcatgccaaa gcgttcgatt ccgctcctga tcgtcctcaa cctgttcttc
      901 ccgctcatct accacttcta ctggctgtac aagaccaagc acgagatgac cgaccggggc
      961 gccgacatcc cgaccaccct gctcgcgttc atcccgttcg tgcactatta cttcctctgg
     1021 aagtggtcgg tgggcgtcga gcaggtcacg cgcggcaaga tgtcgtccgc catcgcgttc
     1081 gtgctgatgt tcttcatcgg gtggatcatc gggccggcga tcatccaggc gaccctcaac
     1141 gaggtgcccg aggagggcgt gaacctgccg caggcccgcg tcgtcgccta ggtggcagcg
     1201 ccgctcgcct acgccctggc gatcgtcgtg ctgtggctgg cgggccgggc gctgcgccgc
     1261 gcgcagccgt aggggcttga ccgaggcgcg catccgtcgg aaacgtgagg gcgatggacc
     1321 gaccgccgcc gagcgccggg ggacgccgcg agcgcgtggt gcacgggctg atcgtcgcgg
     1381 cggtcgcggc ggtgctcagc ctcgcgtcca ccgcgcccta cggcatcctc aaccaggcga
     1441 cctacctgct cgatccgctg catcgcgcgt tccccgagct gttccaccgc gactggctga
     1501 tcaccgaggc cccgccgtac ttgccgagct tcggctggct ggcgcggtgg ctgtacgccg
     1561 ccgatccgga cggcgcggtc gcgatgctgg tcgcgaactt cgcggtgacg ttcgccacct
     1621 acgtcgcgct ggactggctg gtcaccgcgg tgtgcccgag ccggcgcggc ttcgtgctgg
     1681 tcgcgagctt cgtcaccgtg acaatgggcc gcgcgctggg cggcagctac ctgctcgcgg
     1741 gctacctcca gccgtccgcg cccgcgacgc tgggctggat cgtcgcgatg gcggcgctgg
     1801 tccgcggccg ctaccggacg tgcggcgtcg cgctggcggt ggccggtgcg atgcacatca
     1861 acttcctggt cctgggcatc gggctgttca cgctgggggc gatcgcgcgg cgcggcgcac
     1921 ggctcgtcga tctcgccggg ctgctcgtgc cgcagctcgt ggtgctggcg tggctgctgc
     1981 cggggctcgt cgcatcggcc ggaccgggcg acgatgcgct gcggatcctc gtgcagttcc
     2041 acgcgccggg ccattacctc ggccgccggc tgatctcgtg ggtgcccggc ctcgtcggct
     2101 ggcagctcgc cgggttcgcc gcgctgcccc cggccggcgc gcggagcccc gcgctggtcg
     2161 cgctgtggcg gttctcgctg gcgtcgtgcg tgatcgcggt cgcggccgcg ttcgtcgtcc
     2221 acgtgccggc gttcgagtgg ctgacccagg cgttctggcc gcggatcgcg ccgttcggcc
     2281 agctcggctg ccaggtgctg atcgcggcgg cgctggtccg ccagggcacg gccccggtct
     2341 cggtcgcgcg ccgcgcgtgg ctggccggcg cgat
//
LOCUS       DASVOR010002258         4112 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_199939,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002258 DASVOR010000000
VERSION     DASVOR010002258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4112
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199939"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VF516_41825"
     CDS             <1..346
                     /locus_tag="VF516_41825"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEX8114364.1"
                     /translation="QPRHLKIELGSIWYGPIAQGTGVSAETAHLLAGHAVALGYRRVE
                     WKCDAKNQRSRRAAVAYGFAFEGVQDAHYIVKGKSRDTAWYRMLDTEWATFRDRLAGY
                     AASRGWKTTGPT"
     gene            complement(350..1579)
                     /locus_tag="VF516_41830"
     CDS             complement(350..1579)
                     /locus_tag="VF516_41830"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8114365.1"
                     /translation="MTATYRVPGAILTEREHTVPLRHGAPGGETLTVFTREVAAPDGA
                     NRPYLVFLQGGPGFEATRPTSPPSGWIARALADYRVLLLDQRGTGRSSPVTSQLPGAT
                     PEAQADYLTQFRADAIVRDAEHIRRQLGIARWSVLGQSFGGFAAMTYLSIAPDGLAEV
                     FITGGLSPIGRPVDDVYRATYARLVEKNRAYFERYPDDRARTRTLLARLDAEDVRLPS
                     GDRLTARRFRQLGLWLGDSAGFELLHHVLELPFGSPAFLHDVEAGVRFARNPIYATLH
                     ESSYADGGATRWSAARLYPDEIAAEGYFTAEQVFPWMWEDYGALRPHAAAAERLAAHP
                     WPRLYDADQLRRNEVPVAATIYVNDLYVERRFAEDTAAAIRGLRPWITNELEHNGLRA
                     DGERVLGRLIDLVRGRA"
     gene            complement(1618..3648)
                     /locus_tag="VF516_41835"
     CDS             complement(1618..3648)
                     /locus_tag="VF516_41835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114366.1"
                     /translation="MHLSRLTTTHRAPPWVLVPLLTALMACGGSSAPEVVVTISPATA
                     SVNAGQTVQFTATVSGSNNTKINWSAGGGTVTAGGLFTAPALGGSYTVRATADANPKD
                     SATGLVNVMGNDSAPLEPFYDSAHPYVQVMTPMPFATYFAPATIRMWAHAPDQGSDEV
                     NDGYSPRVDFYLGTQMVGSVAATGAADYYQLDIPNVAAGTYELSVRSLLKSGTVESVH
                     VPVKVIDPPSGAGITTMTLTSDLVLSGSMNFELIGTADKPAVLISTNGSRIRSADGWT
                     GHLKIQHADILGLGKMDTPGIEVTVSGSNALEISDSIFDACGPPALGANDQATIAFNR
                     NTLQPNILTPVNDQPDYSDSHPSLVFTGSSKAPKTFQGNNIGVSFVRFDHSSNWTVGG
                     DHDADGNIIIGVRGGLEFDSVSNVTIRGNFSYHRYPFGWSQGHDLDFEGADENTGTFT
                     IEHNVFRSSSWMIQSLPSGTAFQYNLLLDNINEAFFRDYGASVKVHHNVLVNSGWQRT
                     FLPSGGVLSASGEFTNNTVDAGGAQLGWVQGSFVANNSSLSALRNNVFTGFAYDSAVD
                     LVASGAAAAADHNCFFNPDTTKLRPYGTAGLGGGDVTADPKFANPRAIPAPIRDGDVW
                     RRRLTVSQILATYRSIYKPGGSSPLAGAASDGGNIGAIGTNGADDLFGKFGP"
BASE COUNT          570 a         1373 c         1550 g          619 t
ORIGIN      
        1 ccagccgcgg cacctcaaga tcgagctcgg cagcatctgg tacgggccga tcgcgcaggg
       61 caccggggtg tcggcggaga ccgcgcacct gctcgccggc cacgcggtcg cgctcggcta
      121 tcgccgggtc gagtggaagt gcgacgccaa gaaccagcgg tcgcggcgcg ccgcggtcgc
      181 ctacggcttc gcgttcgagg gcgtgcagga cgcgcactac atcgtcaagg gcaagagccg
      241 cgacaccgcg tggtaccgca tgctcgacac cgagtgggcg acgttccgcg atcggctcgc
      301 cggctacgcg gcgtcgcgcg gctggaagac gaccggaccg acctgacgct caggcgcggc
      361 cgcgcaccag gtcgatcagc cggccgagca cgcgctcgcc gtcggcgcgc agcccgttgt
      421 gctcgagctc gttggtgatc cacgggcgca ggccgcggat cgccgccgcg gtgtcctcgg
      481 cgaaccggcg ctcgacgtag aggtcgttga cgtagatcgt cgccgcgacc gggacctcgt
      541 tgcggcgcag ctggtcggcg tcgtacagcc gcggccaggg gtgggcggcg agccgctcgg
      601 cggcggcggc gtgcggacgc agcgcgccgt agtcctccca catccacggg aacacctgct
      661 cggcggtgaa gtagccctcg gcggcgatct cgtcggggta gagccgcgcc gccgaccacc
      721 gggtcgcgcc gccgtcggcg tacgacgact cgtgcagcgt ggcgtagatc gggttgcggg
      781 cgaaccgcac gccggcctcg acatcgtgga ggaacgccgg cgagccgaac ggcagctcga
      841 gcacgtggtg caggagctcg aacccggcgc tgtcgccgag ccacagcccg agctggcgga
      901 accgccgcgc ggtcagccgg tcgcccgacg gcagccggac gtcctccgcg tcgagccggg
      961 cgagcagcgt gcgggtccgg gcgcggtcgt ccgggtagcg ctcgaagtac gcgcggttct
     1021 tctcgaccag ccgcgcgtag gtcgcgcggt agacgtcgtc gaccggccgg ccgatcggcg
     1081 acagcccgcc ggtgatgaac acctcggcca ggccgtccgg ggcgatcgac aggtaggtca
     1141 tcgccgcgaa cccgccgaag ctctggccga gcacgctcca gcgcgcgatg ccgagctggc
     1201 ggcggatgtg ctcggcgtcg cgcacgatcg cgtcggcgcg gaactgggtc aggtagtcgg
     1261 cctgggcctc gggggtggcg ccgggcagct ggcttgtgac cgggctcgac cggccggtgc
     1321 cgcgctgatc gagcaggagc acgcggtagt cggcgagcgc gcgcgcgatc cagcccgacg
     1381 gcgggctggt cggccgcgtc gcctcgaacc ccggcccgcc ctggaggaac accaggtacg
     1441 gccggttcgc cccgtcgggc gcggcgacct cgcgggtgaa cacggtgagg gtctcaccgc
     1501 cgggcgcgcc gtggcgcagc gggacggtgt gctcgcgctc ggtgaggatc gcgccgggga
     1561 cgcggtaggt ggcggtcatc gcgccgacga tagcgcggcg cgccggcggc gggcgggcta
     1621 cgggccgaac ttgccgaaca ggtcgtcggc gccgttggtc ccgatcgcgc cgatgttccc
     1681 gccgtcggac gcggcgccgg cgagcgggct cgagcctccc ggcttgtaga tgctgcgata
     1741 ggtcgcgagg atctgcgaca cggtcaggcg gcgccgccac acgtcgccgt cgcggatcgg
     1801 ggccgggatc gcacgaggat tggcgaactt cggatcggcg gtcacgtcgc cgccgccgag
     1861 ccccgcggtg ccgtacggtc tcagcttcgt ggtgtccgga ttgaagaagc agttgtggtc
     1921 ggccgccgcc gccgcgccgc tcgcgaccag gtcgaccgca ctgtcgtacg cgaacccggt
     1981 gaacacgttg ttgcgcagcg cgctgagcga cgagttgttc gcgacgaacg agccctggac
     2041 ccagccgagc tgtgcgccgc cggcgtcgac cgtgttgttg gtgaactcgc cggacgcgga
     2101 cagcacgccg ccgctgggca ggaacgtccg ctgccagccc gagttgacca gcacgttgtg
     2161 gtggaccttc accgacgcgc cgtagtcgcg gaagaacgcc tcgttgatgt tgtcgagcag
     2221 gaggttgtac tggaacgcgg tgccgctggg caggctctgg atcatccacg agctcgagcg
     2281 gaacacgttg tgctcgatcg tgaacgtgcc cgtgttctcg tcggcgccct cgaagtcgag
     2341 gtcgtggccc tgcgaccagc cgaacgggta gcggtggtag gagaagttgc cgcggatcgt
     2401 gacgttgctg acggaatcga actccaggcc gccgcgcacg ccgatgatga tgttgccgtc
     2461 ggcgtcgtga tcgccgccga ccgtccagtt gctggagtgg tcgaaccgca cgaacgacac
     2521 gccgatgttg ttgccctgga acgtcttggg cgccttcgag ctgccggtga acaccaggct
     2581 cggatgcgag tcgctgtagt ccggctgatc gttgaccggc gtgaggatgt tgggctgcag
     2641 cgtgttgcgg ttgaacgcga tcgtcgcctg gtcgttggcg ccgagcgccg gcgggccaca
     2701 cgcgtcgaag atcgagtcgc tgatctccag cgcgttgcta ccgctgaccg tgacctcgat
     2761 gccgggggtg tccatcttgc cgagcccgag gatgtcggcg tgctggatct tgaggtggcc
     2821 ggtccagccg tccgcgctgc ggatccgcga gccgttcgtg ctgatcagca ccgcgggctt
     2881 gtcggcggtg ccgatcagct cgaagttcat gctgccgctc agcaccaggt cgctggtcag
     2941 ggtcatcgtg gtgatcccgg cgcccgacgg cggatcgatc accttgaccg gcacgtggac
     3001 gctctcgacc gtgcccgact tcagcaggct gcgcaccgac agctcgtacg ttcccgccgc
     3061 cacgttcggg atgtcgagct ggtagtagtc ggccgcgccg gtcgccgcca cgctgccgac
     3121 catctgggtc ccgaggtaga agtcgacgcg cggcgagtag ccgtcgttga cctcgtcgct
     3181 gccctggtcc ggcgcgtgcg cccacatccg gatcgtcgcc ggcgcgaagt aggtcgcgaa
     3241 cggcatcggc gtcatcacct gcacgtacgg gtgcgccgag tcgtagaacg gctcgagcgg
     3301 cgccgagtca ttgcccatga cgttgaccag gccggtcgcg ctgtccttgg ggttggcgtc
     3361 ggcggtggcg cgtacggtgt agctgccgcc gagggccgga gcggtgaaca gcccgcccgc
     3421 ggtcacggtg ccgccgcccg cgctccagtt gatcttggtg ttgttgcttc cggacacggt
     3481 ggcggtgaac tgcacggtct ggccggcgtt gacgctggcg gtcgccggcg agatcgtgac
     3541 cacgacctcc ggtgcgctgc tgccaccgca cgccatcagc gcggtgagca ggggcacgag
     3601 gacccacggc ggggcgcgat gggtcgtggt gagtcgtgac aggtgcaagc gcggcatact
     3661 cgatctccag gttctcgttg ccacgacctc gcgtcaagtc gtggacgagt ccagacacgt
     3721 tgcgatcgcg tgaaaccgct cgacgaggac agcgcgagga cgacgtgatc ggaactgcat
     3781 gacacagcaa tcgttgccca tcggcggcag cgccatcacc ggcggcgcgc gctccggagc
     3841 gcggctcgat cggcagcggc gccggtgcgc ggaatgcgcc gacatcggac atggcgattg
     3901 cgagtcgggg tactctgccg cgcggcggtc tgccgcaact tgccaggagg ctcatgtcca
     3961 gcgaggaact catcgagggc gtgccgtgtc gtgcggcacc gggcgggcgc gcccgctgag
     4021 ccatggccgg gtcctggcac aactggtcgg gcagcgtggt cgccgcgccg gcgcggatcg
     4081 cggcgccgcg cagcgaggac gagctcgcgg cg
//
LOCUS       DASVOR010002259         3130 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_141260,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002259 DASVOR010000000
VERSION     DASVOR010002259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141260"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            919..2001
                     /locus_tag="VF516_41840"
     CDS             919..2001
                     /locus_tag="VF516_41840"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEX8114367.1"
                     /translation="MLSRASRVSADRGAPELHTRFSREFGLRVPLASAGMAFVGLTDL
                     TSAVSNAGALGVYGVGPEPPPVVAARIQAIRDQTSGPFGVDFIVASSPLGTFTTQDHI
                     DVVAAARVPLVVFHWNLPTQAWVTQLHAAGIKVFIQTGDVRVALQGVALGADGVIAQG
                     RAAGGHNRNSTIPTLRVVDMMRRALPHEIFILAAGGISDGRSLVRAIRAGADGGWAGT
                     VFVAAEESNAHDGYKARLIAAHSPEATTFTTVFGPEFPDAQQRVLRNHATAHPDATTP
                     PTIGTTTLFPGVLNVPYTMPLHSAIVPTRDTQGDLDEMDMPAGAVSVRAVRRVRPAAD
                     IVEDFIAGAHEAIERGDRDDRSIDDD"
     gene            2287..2901
                     /locus_tag="VF516_41845"
     CDS             2287..2901
                     /locus_tag="VF516_41845"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX8114368.1"
                     /translation="MTATTDDLCSHVAAHAGVPTGLAEQATRAVLAGMGGTLTPAGRE
                     LVADELPAGLGAAILAGGSPAMPIEDPLLGLGVTPGQARELIASVCRVLVEELSSEAV
                     DALRVGVPTALAALLVEPVTDEPPPRPALGPRHDTLADGHPGSHHPLSDAPGRGAQRG
                     SIADDNPHAATKLSSTAGSSQERRRETLAEGRPGARYPLASGRG"
BASE COUNT          461 a         1122 c         1102 g          445 t
ORIGIN      
        1 cccctcgcgc gtcgcgcgtc gcgccctgcg ccgccgccgg gccgatccca tcgcgatcgc
       61 agcggggcaa tttcgtgcat cgccgcgggg cgcggctccc acggcgcgac cctgtccgga
      121 tccgggacag ctgcgtgaca ccggatgcgc acgggtagcg ccggggttgc gctctcgcgc
      181 cgggtagcac cggggttgcg ctctcgcgcg cagcttgacg cccgaggacc gacatggcga
      241 catgagactg ttccatgaat gggtcaggcg gacggcacga tgcgacagcc acggcgccgc
      301 ccgctcgtca cgcgccgcgg caacgcgatg atcgagacat ggcgtcatcg tgcaaaagcg
      361 agagtcagct cgcgcggccg cgcgtcgcag tcgcaggcgc gcaccgccga gatctgacga
      421 catgcgcagc agatgcatac cgcgccgaga caaaggtgag ctgcgttgcg atcgcatccc
      481 cccgagtggg ccggcgagaa aactccggtc cacatgtaac ggtgaggagc agcgcacatg
      541 tcatgcgtgg gcgttcgggc gtgcaatctt cgtcgcgcag ctgccgcgct ttgccgttcc
      601 gcattcgggt tcagaccgac gagactgctc gccggtggag cgccggccgg tggatcgctg
      661 gatcgatcac gtacacaaag cgatgccacc acaccgcacc gcgttccgcc atcccatccg
      721 ccggtgcgcc gtatcggcca atacgcggca ccgatcgcaa taccaactcc tcagtgatgc
      781 catctgctgc gaacagcaat cctggcagcc atcgcgtaca cctatcgtgg gggttcaaac
      841 atgtcatcca acgagtcgaa gacgaggccg agtcgcagga ccatcctgaa gctcgctggc
      901 gtcggagccg gcgccgccat gctgtcccgc gcatcgcggg tttccgcgga tcgcggcgcg
      961 cccgagctgc acacgcggtt ctcacgcgag ttcggactgc gtgtcccgct ggccagcgcc
     1021 ggcatggcgt tcgtcgggct gaccgacctc acgtcggcgg tgagcaacgc cggcgcgctc
     1081 ggcgtgtacg gggtcggccc ggagccgccg ccggtggtcg cagcgcgcat ccaggcgatc
     1141 cgggaccaga ccagcggtcc gttcggggtc gactttatcg tggcgtcatc accgcttggc
     1201 acgttcacga cgcaggacca catcgatgtc gtcgcggccg cccgcgtgcc gctcgtggtg
     1261 ttccactgga acctacccac gcaggcgtgg gtcacacagc ttcacgcggc cgggatcaag
     1321 gtcttcatcc agaccggcga cgttcgcgtc gcgttgcagg gcgtggcgct cggcgccgac
     1381 ggcgtgatcg cgcagggtcg cgctgcgggc ggtcacaacc gcaactcgac gattccgacc
     1441 ttgcgggtcg tggacatgat gcgtcgtgca ttgcctcacg aaatcttcat cctggctgca
     1501 ggaggtatct ctgacggaag gagcctggta cgagcgatcc gcgccggcgc cgacggcggg
     1561 tgggccggaa cggtgttcgt ggccgccgag gagtccaacg cgcacgatgg ctacaaggct
     1621 cgactgatcg ccgcgcactc gccggaagcc acgaccttca cgacggtgtt cggccccgag
     1681 ttcccggatg cccagcagcg cgtgctgcgc aaccacgcga ccgcgcaccc ggacgcgacg
     1741 acgccgccga cgatcggcac caccacgctg ttccccggtg tgctgaacgt cccgtacacg
     1801 atgccgctgc acagcgcgat cgtcccgact cgcgacacgc agggcgatct cgacgagatg
     1861 gacatgcccg ccggagcggt gagcgtgcgc gcggttcgcc gggtccgccc ggccgccgac
     1921 atcgtcgagg acttcatcgc gggcgcgcac gaggcgatcg agcgcgggga ccgcgacgat
     1981 cgctcgatcg acgacgactg acgcacgcgg ttcgacgcgc cggtgagccc gccggccgag
     2041 cgtcgccccg cgcaaggggt gcgatgctcg atacgtcctg cgcggagtgg acgctccgca
     2101 tcccgcgcag gcggtgatgc tcgccacgtc gtgcgcggga ccggcagatc cgcatttcgc
     2161 gcatgcggtg cgagcgctcg gctcggcgtg cgcggaatcc acggatcggc atctgcccgt
     2221 gttgcgaggc tcggctcgtc ctgcggttgc gcggtcgggt gcggctcttg cttcaccgga
     2281 tccgtgatga ccgcgaccac cgatgacctg tgttctcacg ttgccgcgca tgccggggtg
     2341 ccgaccgggc tcgcggagca agccacccgg gccgtgctcg cggggatggg cggaacgctg
     2401 acgccggccg ggcgcgagct cgtcgccgac gagctgccgg cggggctcgg cgcggcgatc
     2461 ctcgccggcg gttcaccggc gatgccgatc gaggacccgc tgctcgggct cggcgtgacc
     2521 cccggacagg cgcgcgagct gatcgccagc gtgtgccggg tgctcgtcga ggagctgtcc
     2581 agcgaagcgg tcgacgcgct ccgcgtcggg gtcccgacgg cgctcgccgc gctgctcgtc
     2641 gagccggtga ccgacgagcc accgccgcgc ccggcgctgg gcccgcggca cgacacactc
     2701 gccgacggcc accccgggag tcatcacccg ctgagcgatg cccccggccg cggcgcacag
     2761 cgcggatcga ttgccgacga caatccccac gcggccacca agctgtcgag caccgccggc
     2821 tcgagccagg agcgccgccg cgagacgctc gccgagggca ggccgggggc tcggtacccg
     2881 ctggccagcg gccgcggctg atcgcgcagc gcagccccgg gcggcgcggg cattcgcgtc
     2941 cccggcattc ccccgctccg cgtcgcggtc gattccgtgg atgaccccgc gcgacgcgac
     3001 gcgcggaggg gtgaatggac cggcggtgtc ggtccagagg ggacgggaag gtcccctgtg
     3061 gaaagtcccc tgtggatcga tatccccgcg cgcccacgca caggcgcgct tccggagcgc
     3121 acgaccgtcc
//
LOCUS       DASVOR010002260         1964 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_345325,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002260 DASVOR010000000
VERSION     DASVOR010002260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345325"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..848)
                     /locus_tag="VF516_41850"
     CDS             complement(195..848)
                     /locus_tag="VF516_41850"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114369.1"
                     /translation="MAKLEKAQLRELDETLENEVGSTPVTVQFNPDTLKVSYANQVKQ
                     PEGAGDQRGGASQLFVGAGTTKMTCQLWFDVTAPNGDNQPSEDDVRKLTTKVSFFITP
                     KEDQGKKGFVPPGVRFLWGSFQFDGIMESVEESLEYFSPDGKPLRASVSIALSQQKIL
                     VFPPIPANGKPTPGSTLARPVPSGKSVQQVAGKNWQSTAARNGIENPRRPPPGQRLR"
     gene            complement(851..1348)
                     /locus_tag="VF516_41855"
     CDS             complement(851..1348)
                     /locus_tag="VF516_41855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8114370.1"
                     /translation="MADFDAQLAFTAFNFAVEIQLPGVDGVSRPLCKGSFSECDGLEL
                     AFDVKTIREGGNNSRQIRLAGPVTLGTLSLKRGMTPGFDLWTWVKSVQQNPALRAEAV
                     VVMFDADRSKQPDVRARFVLSRCLPVKIKAPALNAKDGLIAVEELQVAYEALELDLST
                     TGQAG"
     gene            complement(1351..>1964)
                     /locus_tag="VF516_41860"
     CDS             complement(1351..>1964)
                     /locus_tag="VF516_41860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114371.1"
                     /translation="HPWLVGDDDPGTGALPILPPDGAVCGMIAQRALARGAWIAPANE
                     PLRGVVELTPSLPRDDASRLADSQVNLLGDEPHGIVALAADTLARDPDLTGIHVRRLL
                     ILLRRIALRVGPGYVFEPNGAVFQRLVRRGFEAVLDDLFARGAFAGDERAAAYQLAVD
                     TTASLADQGRFVVEMKVAPSQPMSFLTVRLIQSGDVGVVSEAR"
BASE COUNT          272 a          707 c          679 g          306 t
ORIGIN      
        1 cgcgcgcctc gcgttcggcg agccggcgca gcgccggcgc gagctcgcgc ggatcgatcc
       61 gcggtggttc ggaggagccg gccgccgcgc tcgatgcggc gggcggtgcc accggcgggg
      121 ccgactgcgg aatgacctca acggtgccaa agctgaccgg catggttcct cctgtgcatc
      181 acgtccctca cgcatcacct cagccgctgc ccgggtgggg gccgccgtgg gttctcgatc
      241 ccgttgcgcg ccgcggtcga ctgccagttc ttcccggcga cctgctgcac cgacttcccg
      301 ctcgggaccg ggcgcgccag cgtgctgccc ggcgtcggct tgccattggc cgggatcggc
      361 ggaaacacca ggatcttctg ctggctcagc gcgatcgaga cgctggcgcg cagcggcttg
      421 ccgtcgggcg agaagtactc cagcgactcc tcgaccgact ccatgatgcc gtcgaactgg
      481 aatgaccccc acaggaagcg cacgccgggc ggaacgaacc ccttcttgcc ctggtcctcc
      541 ttgggcgtga tgaagaagct caccttggtc gtcagcttgc ggacgtcgtc ctcgctcggc
      601 tggttgtcgc cgttgggcgc cgtgacgtcg aaccacagct ggcaggtcat cttggtcgtt
      661 ccggcgccca cgaacagctg cgatgcgccg ccgcgctgat cgccggcgcc ctcgggctgc
      721 ttgacctggt tcgcgtagga caccttgagg gtgtcggggt tgaactggac ggtgaccggc
      781 gtcgacccga cctcgttctc cagggtctcg tcgagctctc gcagctgggc tttctcgagc
      841 ttggccatgg ctatcccgcc tgtcccgtgg tggagaggtc gagctcgagc gcctcgtagg
      901 cgacctggag ctcctcgacg gcgatcagcc cgtccttggc gttgagcgcc ggcgccttga
      961 tcttcaccgg cagacagcgc gacagcacga accgggccct gacatcgggc tgcttgctcc
     1021 ggtcggcgtc gaacatcacc acgaccgcct cggcgcgcag tgccgggttc tgctgcacgc
     1081 tcttgaccca ggtccacagg tcgaaccccg gcgtcatgcc gcgcttgagc gacagcgtgc
     1141 ccagggtcac cggaccggcg agccggatct ggcggctgtt gttgccgccc tcgcggatgg
     1201 tcttgacgtc gaacgccagc tcgagcccgt cgcactcgga gaacgagccc ttgcacagcg
     1261 gccgcgaaac gccgtcgacg ccgggcagct ggatctcgac cgcgaagttg aacgcagtga
     1321 acgcgagctg ggcatcgaag tctgccatgg ctagcgcgcc tccgagacca cgccgacgtc
     1381 gccgctctgg atcagccgca cggtcagaaa gctcatcggc tgcgacggtg cgaccttcat
     1441 ctcgaccacg aaccggccct ggtcggccag gctcgccgtg gtgtcgaccg cgagctggta
     1501 ggccgccgcg cgctcgtcgc cggcaaacgc gccgcgcgcg aacaggtcgt cgagcaccgc
     1561 ctcgaagccg cgccgcacca gccgctggaa cacggcgccg ttgggctcga acacgtaacc
     1621 cggcccgacg cgcagcgcga tccggcgcag caggatcaac agccgccgca cgtggatccc
     1681 cgtcagatcg gggtcgcggg ccagcgtgtc ggcggcgagc gcgacgatgc cgtgcggctc
     1741 atcgccgagc aggttgacct ggctgtcggc gagccggctc gcgtcgtcgc gcggcaggct
     1801 cggggtcagc tcgaccacgc cgcgcagcgg ctcgttcgcg ggtgcgatcc aggccccccg
     1861 cgccagcgcg cgctgggcga tcatgccgca caccgcgccg tcgggcggca ggatcggcag
     1921 cgcgccggtg cccggatcgt cgtcgccgac cagccacggg tggt
//
LOCUS       DASVOR010002261         2433 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_520868,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002261 DASVOR010000000
VERSION     DASVOR010002261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2433
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520868"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VF516_41865"
     CDS             <1..194
                     /locus_tag="VF516_41865"
                     /inference="COORDINATES: protein motif:HMM:NF020907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HEX8114372.1"
                     /translation="NRAYLERHGFLFIVCATGRSADAMLADLRARTPRSLADELRTAA
                     EEQLQITRLRLHKLLGELA"
     gene            191..523
                     /gene="uraH"
                     /locus_tag="VF516_41870"
     CDS             191..523
                     /gene="uraH"
                     /locus_tag="VF516_41870"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HEX8114373.1"
                     /translation="MITTHVLDTAAGRPGRGIAIELERHDAGSWHRIGAGVTDDDGRL
                     RTLTPAGPVAPGVYRLRFHTGAYFATQQQAGFFPVVEIQFTVADGAQHHHVPLLLSPY
                     GYSTYRGS"
     gene            649..1995
                     /locus_tag="VF516_41875"
     CDS             649..1995
                     /locus_tag="VF516_41875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114374.1"
                     /translation="MSVRTSLPEKKLQKILGRIRRKQARISERYPGESGARQPVHTVY
                     GGAHLFTSDTARKLGDLALRALDSHAPDARSFADAIGVPAELAPKVYDRVRDKLRREP
                     VEDFRIDFEDGYGNRPDDEEDGHAVAAAKQVAAGMVAGTLPPFLGIRVKPLTGELAGR
                     SLRTLDVFLTELLANTNGTLPSNFVVTLPKIQSRIDVEVLIDGFEQLEDRLDIENGAL
                     RVEFMVETTQSLFDPQGRAVLPRLHRAAGGRLTGAHFGTYDYTAGCDITAAQQRMRHP
                     ACDFAKQMMKVAFAGTGVMLSDGATNIMPVGPHRGASLTEDQQRANRAAVFAAWRLHA
                     DDIRHSLVHGFYQGWDLHPAQLPSRYGAIYGFFLDGFGPASERLRNFVDKAAQATLVG
                     DVFDDAATGQGLLNYFLRGIACGAVTEHEALTTGLSLDEIRSKSFVKIMAARRAQS"
     gene            2085..2162
                     /locus_tag="VF516_41880"
     tRNA            2085..2162
                     /locus_tag="VF516_41880"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2119..2121,aa:His,seq:gtg)
     gene            complement(2168..>2433)
                     /locus_tag="VF516_41885"
     CDS             complement(2168..>2433)
                     /locus_tag="VF516_41885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEX8114375.1"
                     /translation="SVLALADVAGPLGDAVITADGACDGVVLDAPRGTGGFGYDPLFL
                     VPELGRTFAELGVGTKSERSHRARAMQAIKPRLLAYLAQLGAV"
BASE COUNT          366 a          910 c          840 g          317 t
ORIGIN      
        1 ccaaccgcgc gtacctcgag cgccacggct tcctgttcat cgtgtgcgcg accggccggt
       61 ccgccgacgc catgctcgcc gacctgcgcg cccgcacgcc gcgctcgctc gccgacgagc
      121 tgcgcaccgc cgccgaggag cagctccaga tcacccggct gcgcctccac aagctgctcg
      181 gagagctcgc atgatcacca cccacgtcct cgacaccgcc gccggccgcc ccggccgggg
      241 gatcgcgatc gagctcgagc gccacgacgc cggcagctgg caccggatcg gcgccggcgt
      301 caccgacgac gacggccggc tgcgcacgct gaccccggcc ggccccgtcg cccccggcgt
      361 ctaccggctg cgcttccaca ccggcgcgta cttcgccacc cagcagcagg ccggcttctt
      421 cccggtggtc gagatccagt tcaccgtcgc cgacggcgcc cagcaccacc acgtgccgct
      481 gttgctctcg ccctacggct actcgaccta ccgcggcagc tgaggactcc atcggcccgg
      541 cgggcgcgcc cggcggggca ggcgcacggg cgaggtcatc gcgccggtgc cgcagaacgc
      601 gacaaaacag ccacctactg tgggctgggt cagtgtacaa accgcgccat gtcggtccgc
      661 acctcgctcc ccgagaagaa actgcagaag atcctcggcc ggatccgccg caagcaggcg
      721 cggatcagcg agcgctaccc cggtgagtcg ggggcgcgcc agccggtgca cacggtgtac
      781 ggcggcgcgc acctgttcac gtcggacacc gcgcgcaagc tcggcgacct cgcgctgcgc
      841 gcgctggaca gccacgcgcc cgacgcccgg agcttcgccg acgcgatcgg cgtgcccgcc
      901 gagctcgccc ccaaggtcta cgaccgggtg cgcgacaagc tgcggcgcga gccggtcgag
      961 gacttccgga tcgacttcga ggacggctac ggcaaccggc cggacgacga ggaggacggc
     1021 cacgcggtcg cggcggccaa gcaggtcgcg gccggcatgg tcgccggcac gctgccgccg
     1081 ttcctcggca tccgggtaaa gccgctgacc ggcgagctcg cggggcgctc gctgcgcacg
     1141 ctcgacgtgt tcctgaccga gctcctggcc aacaccaacg gcacgctgcc gtcgaacttc
     1201 gtggtcacgc tgcccaagat ccagagccgg atcgacgtcg aggtgctgat cgacggcttc
     1261 gagcagctcg aggaccggct cgacatcgag aacggcgcgc tgcgcgtcga gttcatggtc
     1321 gagaccacgc agagcctgtt cgacccgcag ggccgcgcgg tgctgccgcg attgcaccgc
     1381 gccgccggcg gccggctgac cggcgcgcac ttcgggacct acgactacac cgccggctgc
     1441 gacatcaccg ccgcgcagca gcggatgcgg cacccggcgt gcgacttcgc caagcagatg
     1501 atgaaggtcg cgttcgccgg caccggcgtg atgctgtccg acggggcgac caacatcatg
     1561 ccggtcggcc cgcaccgcgg cgcgagcctg accgaggacc agcagcgcgc caaccgcgcc
     1621 gcggtgttcg cggcgtggcg gctgcacgcc gacgacatcc gccactcgct ggtccacggg
     1681 ttctaccagg gctgggacct gcacccggcg cagctgccga gccgctacgg cgcgatctac
     1741 gggttcttcc tcgacggctt cgggccggcg tccgagcggc tgcgtaactt cgtcgacaag
     1801 gcggcgcagg cgacgctggt cggcgacgtg ttcgacgacg cggccaccgg ccagggcctg
     1861 ctcaactact tcctgcgcgg catcgcgtgc ggcgcggtca ccgagcacga ggcgctgacg
     1921 accgggctgt cgctcgacga gatccgcagc aagtcgttcg tcaagatcat ggcggcgcgg
     1981 cgggcgcagt cgtagccccg cactccgcat gccaagtggg cgtcataaca ggtgttcctg
     2041 gaggttgcag gcgcgccccg agtcatgtag acacaggttc ctgcggagga tgtagctcag
     2101 ttggtagagc accaggttgt ggccctgggt gtcgtgggtt cgatccccat cattctcccc
     2161 caccagttca gacggcgccg agctgcgcga ggtaagcgag cagccggggc ttgatcgcct
     2221 gcatcgcgcg ggcacggtgc gaccgctcgc tcttggtgcc gacgccgagc tcggcgaagg
     2281 tccggccgag ctcgggcacc aggaacagcg gatcgtagcc gaacccgccg gtgccgcgcg
     2341 gcgcgtcgag cacgacgccg tcgcacgcgc cgtcggcggt gatcacggcg tcgccgagcg
     2401 ggccggcgac gtcggcgagc gcgagcacgg acc
//
LOCUS       DASVOR010002262         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_170473,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002262 DASVOR010000000
VERSION     DASVOR010002262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170473"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="VF516_41890"
     CDS             complement(<1..271)
                     /locus_tag="VF516_41890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114376.1"
                     /translation="MQHAAIDLGSRESQICIRQPDGTIVEERKLPTRKLPEVFKTWPT
                     SRVVMEASAEAFKIADAALAAGHQVRVVPGKLVRLLGVGDRGVKND"
     gene            554..763
                     /locus_tag="VF516_41895"
     CDS             554..763
                     /locus_tag="VF516_41895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114377.1"
                     /translation="MTGELGRHRERRRGRLLRQVDQVGHVFAALAITDWLEQIVGGME
                     QGIGTRDLIAEVLDLAEDIERSSRW"
     gene            952..1164
                     /locus_tag="VF516_41900"
     CDS             952..1164
                     /locus_tag="VF516_41900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114378.1"
                     /translation="MVRADHAPLEVLEIDRALASERGMHRRQEHLAQAKALVGIVADR
                     DDRVSGGGSELGERTRGQRERSSMAV"
     gene            1500..2306
                     /locus_tag="VF516_41905"
     CDS             1500..2306
                     /locus_tag="VF516_41905"
                     /inference="COORDINATES: protein motif:HMM:NF039426.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HEX8114379.1"
                     /translation="MAPLLLVAVIAAGYFLVAVVASLGELRHLVRDRRTRVIHGLEHA
                     CARLLERQGHTVIAGQTHPGFFELEVVNDGRASAVAVEQATTEAIARIAAGERALALD
                     PRCGTSLLVGAISVSALLVAATIAGLVAGIARGPLILGTAVAALLVWCGARRLGLLVQ
                     RALTVSTSFAAARGHRIARMPSASGDTAMFLVYVQVFESPPPALGSGRFVTTGLAGDH
                     FHRNHRGPGDQGVGTKGSGPGIGPGDRAGRSGQGTRVAGEHAEAQEVAVL"
BASE COUNT          331 a          843 c          835 g          356 t
ORIGIN      
        1 ggtcgttctt gaccccacga tcgcccacgc cgagcaagcg caccagcttg cccgggacca
       61 cccggacctg gtgccccgcg gcgagggctg cgtcggcgat cttgaacgcc tccgccgagg
      121 cctccatcac gacccgactc gtcggccagg tcttgaagac ctccggcaac ttccgagtcg
      181 ggagcttgcg ttcctcgacg atggttccat ccggctggcg gatgcagatc tgcgattccc
      241 tgctccccag atcgatggca gcatgctgca tggctgtcct ccaaggtgtg cgcttcgagc
      301 gcgtttcttt ctgaggcacg gtttaccgcg acctcgttgc cgaggaggac agccgcttct
      361 gctcgtcgag caggggcagg caacggctgc cgcacgggct gtccagccgc agcgaggccg
      421 gagtcaaggc cgccaagcgc cgaggagcga gcgccgcctt gttcgcgcgc tcgtccgtcc
      481 agccgctcca gcgggccagg acggcacgtc gtgggtcggt ccggcgtcat ccttccgacc
      541 tccgcttgag aagttgacgg gcgagctcgg ccgccatcgc gagcgccgtc gcggtcgtct
      601 gcttcgccag gtcgaccagg tgggccacgt gttcgcggcg ctggcgatca ccgactggct
      661 cgagcagatc gtcggcggca tggagcaagg catcggcacg cgcgatctga tagcggaggt
      721 gctcgacctc gctgaggaca tcgaacgcag ttcccggtgg taggcggcgc atgactacca
      781 ccctccttgc gagggctccg gactcgccgc gtcatcggcc cacgggtcgc gagccgcatc
      841 gtcgagcatg aaatggaact ccgcgccgcc gacggagacc tcgctgtcag caacggcggc
      901 ccggggcatc gcggcgacca ggtcggcgcg tgccagcacg agtagacccg cttggtgcgc
      961 gctgaccatg cgccgctgga agtccttgag atcgatcgcg ccctcgcctc cgagcgcggc
     1021 atgcaccgcc gccaggaaca ccttgcgcag gccaaagcgc tggtcggcat cgtggccgat
     1081 cgcgacgacc gcgtgtcggg cggcggcagc gaactcggcg agcgaacacg tggtcagcgc
     1141 gagcgtagct cgatggcagt gtgatgcctg gctgtcctcc tcggccgctc tcgctcgtcg
     1201 agcaggggca gccaacggct gccgcaccgg ctgtccagcc gcagcgaggc cggagtcaag
     1261 gccgcggagc gccgaagggg agccttgacg ctcaggccga gcggaggctc caggctgagc
     1321 agaggcctgc aggaccggtt ccccgggggc tggtgcgggc gtcgccacaa catcccctct
     1381 aggtatgtgc gggacaacgc ccggccaatc cagatcacgg tcgcggcgtc cgaccccatc
     1441 ctgctcgcgg tcgcccccgg cgagttcgcg ctggcgtccc gcgatcgtgt ctcgcgctcg
     1501 tggcaccgct gctgctggtc gccgtgatcg ccgccggcta cttcctggtc gcggtcgtcg
     1561 cgtcgctcgg agagctgcgg cacctggtgc gcgaccgccg cacgcgggtc atccacggcc
     1621 tcgagcacgc ctgcgccagg ctgctcgagc gccagggcca cacggtcatc gccggacaga
     1681 cccacccggg cttcttcgag ctcgaggtcg tcaacgatgg tcgtgcctcc gcggtcgccg
     1741 tcgagcaagc gacgaccgag gcgatcgccc ggatcgccgc cggcgagcgc gccctcgccc
     1801 tcgacccgcg gtgcggcacc agcctgctgg tcggcgcgat ctcggtctcc gcgctgctgg
     1861 tcgccgcgac gatcgccggc ctcgtcgcgg gcatcgcgcg cggcccgctg atcctcggca
     1921 ccgcggtcgc agcgctcctg gtctggtgcg gcgcccgccg gctcggcctc ctggtgcagc
     1981 gcgcgctcac ggtgtcgacc tcgttcgccg ccgcgcgggg gcatcgcatc gcgcgcatgc
     2041 ccagcgcctc cggcgacacg gcgatgttcc tggtctacgt ccaggtcttc gagtcgccgc
     2101 cgccggcgct gggcagcggc cgctttgtca ccaccggcct cgcaggggac cacttccacc
     2161 ggaatcaccg gggaccaggg gaccaggggg tggggaccaa gggatcgggg ccagggatcg
     2221 gaccagggga tcgggccggc agatcgggcc agggaaccag ggtggccgga gagcacgccg
     2281 aggctcagga agtggcggtg ctgtagggcc ggctccgata attcggaccg tcggatggaa
     2341 aagagtgagg gctcagattc ccgcc
//
LOCUS       DASVOR010002263         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_83146,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002263 DASVOR010000000
VERSION     DASVOR010002263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83146"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VF516_41910"
     CDS             complement(<1..1171)
                     /locus_tag="VF516_41910"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114380.1"
                     /translation="MARALGRYELLRPLARGGMAEVYLARRRAAGIEKWLVVKRMRPE
                     RSGDPRFLDLFVREARLSMSLAHQNIVPVFDFGRIDDQVFLAMERVEGKDLGSSLSRA
                     ADHRLPPLLAAFVAAECCQALDYAHQRKGPDGVALGIVHRDVTPRNVLLSWAGEVKLT
                     DFGIAALAGDATSRLLGTPQYMAPEQARSEPIDPRADIYAVGLVLREAIIGVRSRPGT
                     DRDAMLDAARRGALVPWPHLSAPPPALDAELALGSGDHGEPVEPRPDPPPPALIAIIE
                     RATAAAPADRYPDAQSMLEELDAFIVADRAANKGESPARQLAAWLTGVWDGARDDAGS
                     DAAIHAEHLVSFLDDGAIDVLGTGTMRSMAATAAEDVPAPPAPPAVTPAAPAPSAK"
     gene            1229..>1775
                     /locus_tag="VF516_41915"
     CDS             1229..>1775
                     /locus_tag="VF516_41915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114381.1"
                     /translation="MSPRTLLATVCAVAVAVPAAGWGAPAADLVVIWAPGAKREPIED
                     VARRAGAAVIDRSPTASAVPATASILKRGIDAYDALRFDEAWQTLDEVRALADRTGAA
                     GLSQGQLADLFLYRGLVRIQQGELSAYWDELVTSVTIDPVRVLDPARFPPRVATELAR
                     VHEALRDRKRATLAVDAPAGCS"
BASE COUNT          222 a          648 c          683 g          222 t
ORIGIN      
        1 tcttggcgct cggtgccggg gcggcgggcg tgacggccgg aggcgcgggc ggcgccggca
       61 cgtcctcggc cgcggtcgcg gccatcgagc gcatcgtgcc ggtgccgagc acgtcgatcg
      121 cgccgtcgtc gaggaagctg accaggtgct cggcgtggat cgccgcgtcg gacccggcgt
      181 catcgcgcgc gccgtcccac accccggtga gccaggccgc cagctggcgc gccggcgact
      241 cgcccttgtt cgcggcgcga tcggcgacga tgaacgcgtc gagctcctcg agcatcgact
      301 gcgcgtcggg gtagcggtcg gccggcgcgg cggcggtggc gcgctcgatg atcgcgatca
      361 gcgccggcgg cgggggatcc gggcgcggct cgaccggctc gccgtgatcc cccgagccca
      421 gcgcgagctc ggcgtccaga gcaggtggcg gcgccgacag gtgcggccat ggcaccaggg
      481 cgccgcggcg ggcggcgtcg agcatcgcat cacggtcggt gccggggcgc gatcggacgc
      541 cgatgatcgc ctcgcgcagc accaggccga ccgcatagat gtcggcgcgc ggatcgatcg
      601 gctcgctgcg cgcctgctcg ggcgccatgt actgcggcgt gccgagcagc cgcgacgtcg
      661 cgtcgccggc gagcgcggcg atcccgaagt cggtcagctt gacctcgccg gcccacgaca
      721 gcagcacgtt gcgcggcgtg acgtcgcggt ggacgatgcc gagcgcgacc ccgtcggggc
      781 ccttgcgctg gtgcgcgtag tcgagcgcct ggcagcactc ggcggcgacg aacgcggcga
      841 gcagcggcgg caggcggtga tcggccgcgc gcgacaggct ggagccgagg tccttgccct
      901 cgacgcgctc catggcgagg aacacctggt cgtcgatccg gccgaagtcg aacaccggca
      961 cgatgttctg gtgggccagg ctcatcgaca gccgggcctc gcggacgaac agatcgagga
     1021 accgcgggtc gcccgagcgc tccggccgca tgcgcttgac caccagccac ttctcgatcc
     1081 cggcggcgcg ccggcgcgcg aggtagacct cggccatgcc gcctcgtgcc agcggtcgca
     1141 ggagctcgta gcgacccagc gcacgggcca tcgcctacac tatagcgtga ggttgaccct
     1201 cgctccgaac cgtccgaaac tccgggcgat gtctccccgg acgctgctcg ccaccgtgtg
     1261 cgccgtggcc gtggccgtgc cggcggccgg gtggggggcc cccgcggcgg acctggtcgt
     1321 gatctgggca cccggggcca agcgggagcc gatcgaggac gtcgctcggc gggccggcgc
     1381 ggcggtgatc gaccgctcgc cgacggccag cgcggtcccg gccaccgcca gcatcctcaa
     1441 gcgcgggatc gacgcctacg acgcgctgcg gttcgacgag gcgtggcaga ccctcgacga
     1501 ggtccgcgcg ctcgccgatc gcaccggcgc ggccggcctg agccagggcc agctcgccga
     1561 tctgttcctc taccgcggcc tggtcaggat ccagcagggc gagctgagcg cgtactggga
     1621 cgagctggtc acctcggtga cgatcgatcc ggtgcgcgtg ctcgaccccg cccggtttcc
     1681 gccccgggtc gcgaccgagc tggcgcgcgt ccacgaggcg ctccgcgacc gcaagcgggc
     1741 gacgctcgcg gtcgacgcgc cggcggggtg cagcg
//
LOCUS       DASVOR010002264         2380 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_433048,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002264 DASVOR010000000
VERSION     DASVOR010002264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_433048"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VF516_41920"
     CDS             <1..404
                     /locus_tag="VF516_41920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114382.1"
                     /translation="DGAEREVQLALAMAVPLEQPGVLATLSALRLAQGHAEEALAAAE
                     DAMARCHAMSACGMFRGAFVRLAHAEALHATGAHDAARRTIAEARARLLAIADTIAEP
                     SYRTSFLDAVPENARTLALARTWLGDAAPRA"
     gene            complement(407..1372)
                     /locus_tag="VF516_41925"
     CDS             complement(407..1372)
                     /locus_tag="VF516_41925"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8114383.1"
                     /translation="MKHTILFLAANPLGTDRLALDREARAIQIELERSGFRDSFELVT
                     RWAVEPLDLLRELRKLKPTVVHFSGHGGAGVAGAGDRAAGPHRDIVGPHSADGGAPQQ
                     GLLFQGPDGRPQRVSNEALEDAFDAAGSSVKLVVLSTCYSEPQAAALLTRVDCVVGMR
                     GSVRDDAARSFAIGFYGGLGERESVAAAYKQGCAAIRLEGLHDADLPQLKTRSGVEAA
                     RLVLASAPSAAGAGPGRLDGHARAPRRTDGAPMTRDELLARLSSLLPSQFETVLFRAR
                     IPIEHLSGVSAPQAMRAMEAIRYFEQQDRIPELAAMVQEVAAGPR"
     gene            1934..2179
                     /locus_tag="VF516_41930"
     CDS             1934..2179
                     /locus_tag="VF516_41930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114384.1"
                     /translation="MSRSRGGRGAKRRPEDLEALCNQFASFVKSHPGLRIEQINKELG
                     TTTKDLALPIRKLIAEGKVAAKGQKRSTAYFPGRKAK"
BASE COUNT          369 a          863 c          828 g          320 t
ORIGIN      
        1 tcgatggcgc cgagcgcgag gtccagctcg cgctcgccat ggcagtgccg ctcgagcaac
       61 cgggcgtcct ggcgacgctg tcggcgctcc gcctcgccca gggccacgcc gaggaggcgc
      121 tcgccgccgc cgaggacgcc atggcgcgct gccacgccat gagcgcgtgc ggcatgttcc
      181 gcggcgcgtt cgtgcgcctc gcccacgccg aggcccttca cgcgaccggc gcccacgacg
      241 ccgcccggcg caccatcgcc gaggcccgcg cccgcctgct cgccatcgcc gacacgatcg
      301 ccgagccgag ctaccggacg agcttcctcg acgccgtccc cgagaacgcc cggaccctgg
      361 cgctggcgcg cacctggctg ggcgacgctg cgccgcgcgc ctagggctac cgagggcccg
      421 ccgcgacctc ctggaccatc gcggccagct ccgggatccg atcctgctgc tcgaagtagc
      481 gaatcgcctc catggcccgc atcgcctggg gcgcgctgac gccggacagg tgctcgatcg
      541 gaattcgcgc gcgaaacagc accgtctcga actgcgatgg caagaggctc gacagccggg
      601 ccagcagctc gtcgcgcgtc atcggcgcgc cgtcggtccg gcggggcgct ctggcgtgcc
      661 cgtcgagcct gcccgggccg gcgcccgccg cagagggggc cgatgccagg accagccgcg
      721 cggcctcgac gccggagcgg gtcttgagct gcgggagatc cgcgtcgtgc aggccctcga
      781 ggcggatcgc ggcgcagccc tgcttgtagg cggcagcgac cgactcgcgc tctccgagtc
      841 cgccatagaa gccgatcgcg aagctccgcg ccgcgtcatc gcggaccgag ccgcgcatgc
      901 cgacgacgca gtcgacgcgg gtcaacagcg ccgcggcctg cggctcgctg taacaggtgc
      961 tgagcacgac cagcttgacc gacgagccgg ccgcgtcgaa cgcgtcttcg agcgcttcgt
     1021 tcgagacccg ctggggccgg ccgtcggggc cctggaacag caggccttgc tgcggcgcgc
     1081 ccccgtctgc gctgtgcggc ccgacgatgt cacggtgcgg gccggctgcc cggtcgcccg
     1141 cgcccgccac gcccgcgccg ccatggccgc tgaagtggac cacggtgggc ttgagcttgc
     1201 gcagctcgcg aagcaggtcg agcggctcga ccgcccagcg cgtcaccagc tcgaagctgt
     1261 cgcggaagcc gctgcgctcc agctcgatct ggatggcgcg ggcttcgcgg tcgagggcca
     1321 gtcggtcggt gccgagcgga ttggcggcga ggaacaggat ggtgtgcttc atcggcgatc
     1381 tctgcggagc aggaccggca tcacccggca gcgacccaca ggaccagcct atcgcatccg
     1441 cgcggcggac aacatggtcg cagtcagaca acgttgacat cttcccgtgc accgcagtct
     1501 cgtcgtcaac gggggtgatc acgccgcgat cccgctgccg atcgcagcga gctcgccggt
     1561 cgggcgccac gcgacgcatc gggctcttga cgtcctcgcg ctgccacggg tcacggcgcc
     1621 cggtggttct gcaggtcgca gccggcaatg cgacgatcga catcgcggcc gatgagatct
     1681 cgtatatggc tccttgtctc ggatagcaaa cgcatgacct tgacatacgg cgaagtccct
     1741 gatctgctat cgttcaactc gcgaggtcag aatgagcaac ttccagcaag agatgaatca
     1801 ggtggtcgac gggttcgtgg cacagatcac cgaactcgcc cgtcgcgcag ctgtcaacac
     1861 gctcgaggcg acgtttgcca gccatggcgc cggacgcgcg ccgatcgcag ctccccctca
     1921 aggtgctgct ccggtgagcc gctcgcgtgg tggccgcggc gccaagcgcc gtccggagga
     1981 cctcgaggcg ctgtgcaacc agttcgcgtc gttcgtcaaa tctcacccgg gactgcgcat
     2041 cgagcagatc aacaaggagc tcgggacgac gacgaaggat ctcgccctgc cgatccgcaa
     2101 gctgatcgcc gagggcaagg tcgccgccaa gggacagaag cgttccacgg cgtacttccc
     2161 gggccggaag gccaagtagc aaccagcgat cgcggcgatc acgccggggt gttcagcgcg
     2221 ctgcacattc gctgacagcg ctcaccccac cgcgaggcga aatgcccaga tcgcgacgat
     2281 cgcgccgagc agggcgagcg gccggcggcc gcggagcagg acgtcggcgc ggccgagtgc
     2341 gatgatcgcg agcagggcga ggacgccggc gaggacgagc
//
LOCUS       DASVOR010002265         2006 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_403546,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002265 DASVOR010000000
VERSION     DASVOR010002265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403546"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..455)
                     /locus_tag="VF516_41935"
     CDS             complement(159..455)
                     /locus_tag="VF516_41935"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HEX8114385.1"
                     /translation="MAITVEILVQPRASRAKIGPMHDGRIKIAVTAPPVDGEANAAVI
                     ELLARRLGIARGDIAVIAGAGARRKTLRIERVTRAQLEALLEEQAGGQARGRIE"
     gene            complement(455..1282)
                     /gene="proC"
                     /locus_tag="VF516_41940"
     CDS             complement(455..1282)
                     /gene="proC"
                     /locus_tag="VF516_41940"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEX8114386.1"
                     /translation="MSILANHTIGFIGAGNMAEALIRGLVRGGHVPAGRITASGPRRE
                     RLDELARGYGIAVTTDNREVARGAELVVLSVKPQILDKVLREVGGELRPGTLVVSIAA
                     GVDTESIEDAIADGVRGVRAMPNTPALVGAGATAIAPGRHAGEADLATAKALFDAVGI
                     SVVLDEHHLDAVTGLSGSGPAYIFLILEALSDAGVKVGLSRRNAQRLAAQTVLGSAKM
                     LLETDEHVGKLKDMVTSPGGTAIAGLHTLEEGGLRTTLINAVETATRRARELGRGGK"
     gene            complement(1333..>2006)
                     /locus_tag="VF516_41945"
     CDS             complement(1333..>2006)
                     /locus_tag="VF516_41945"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HEX8114387.1"
                     /translation="APRASDSLTGLRASDSLTGLRTPGASSSWSAMPVTSPGGLPRLC
                     VIGISTGGPAALTELVPALPPDLRIAIVVVQHMPAHFTGALAERLDAVSRISVHEAQD
                     GDRPLAGTVLIAPGDRHLEFDDRGAIVLTDGPHVNGCRPAADVTMISAARVYGRRALA
                     VVMTGMGKDGTAGALAIKRADGKTLAQDQASSVIYGMPKAAIDAGAIDEVAALSDIPA
                     WLRYA"
BASE COUNT          242 a          751 c          749 g          264 t
ORIGIN      
        1 gctgcgcgcg gcctcgacga cctggtgcgg ctgggtgatc gtcaggcgca gccgctgcac
       61 cggatcgagc acgtgcgagt ccggcgcggt gaccagctcg accgcgaccg tcccggtggt
      121 gccggtgcag ccgcacagcg caagcgcggc gagcgccctc attcgattcg cccccgggcc
      181 tgccccccgg cctgctcctc gagcagcgcc tcgagctggg cgcgcgtgac gcgctcgatc
      241 cgcagcgtct tgcgccgcgc gcccgcgccg gcgatcaccg cgatgtcgcc gcgcgcgatg
      301 ccgagccggc gggcgagcag ctcgatcacc gcggcgttgg cctcgccgtc gaccggcggc
      361 gcggtcaccg cgatcttgat ccggccgtca tgcatcggac cgatcttggc gcgtgacgcg
      421 cgcggttgca ccaggatctc gacggtgatc gccatcactt gccgccgcgg ccgagctcgc
      481 gggcgcgccg ggtcgcggtc tcgaccgcgt tgatcagcgt ggtgcgcaga ccgccctcct
      541 ccagggtgtg cagccccgcg atcgcggtgc cgcccggcga cgtcaccatg tccttgagct
      601 tgccgacgtg ctcgtcggtc tccagcagca tcttcgccga gcccagcacg gtctgcgccg
      661 cgagccgctg ggcgttgcgc cgcgacagcc cgaccttgac gccggcgtcg gacagcgcct
      721 cgaggatcag gaagatgtac gccggccccg agcccgacag cccggtgacc gcgtcgaggt
      781 ggtgctcgtc gagcaccacg ctgatcccga ccgcgtcgaa cagcgccttg gcggtcgcca
      841 ggtcggcctc gccggcgtgc ctgccgggcg cgatcgcggt ggcgccggcg ccgaccagcg
      901 ccggcgtgtt gggcattgcg cgcacccccc gcacgccgtc ggcgatcgcg tcctcgatcg
      961 actcggtgtc gaccccggcg gcgatcgaca ccacgagcgt gccgggccgg agctcgccgc
     1021 cgacctcgcg cagcaccttg tcgaggatct gcggcttgac gctgagcacg accagctcgg
     1081 cgccgcgcgc gacctcgcgg ttgtcggtgg tgaccgcgat gccgtagccg cgggcgagct
     1141 cgtcgagccg ctcgcggcgc ggccccgacg cggtgatccg gccggccggg acgtggccgc
     1201 cgcgcaccag gccgcggatg agcgcctcgg ccatgttgcc ggcgccgatg aatccgatcg
     1261 tgtggttggc gaggatcgac aaggccccat ggtgtagcac cgccgggccg tgtcgcgcga
     1321 ccgccgcggg cgtcatgcgt agcgcagcca cgcggggatg tcggagagcg cggcgacctc
     1381 gtcgatcgcg ccggcgtcga tcgccgcctt cggcatgccg tagatcaccg agctcgcctg
     1441 atcctgggcc agggtcttgc cgtcggcgcg cttgatcgcg agcgccccgg ccgtgccgtc
     1501 cttgcccatc ccggtcatca ccaccgcgag cgcgcgccgg ccgtagaccc gcgcggccga
     1561 gatcatcgtg acgtcggcgg cggggcggca gccgttgacg tgcgggccgt cggtcaggac
     1621 gatcgcgccg cggtcgtcga actcgaggtg gcgatcgccg ggcgcgatca gcaccgtgcc
     1681 ggcgagcggg cgatccccgt cctgggcctc gtggaccgag atccggctga ccgcgtcgag
     1741 ccgctcggcg agcgcgccgg tgaaatgggc gggcatgtgc tggacgacga cgatcgcgat
     1801 ccggaggtcg ggcggcagcg cggggaccag ctcggtcagc gcggcgggac cgccggtcga
     1861 gatcccgatc acgcacagcc gcggcaggcc gccgggcgag gtcaccggca tcgccgacca
     1921 cgacgacgac gcgccgggcg tgcgcagccc ggtgagcgag tccgacgcgc gcagcccggt
     1981 cagcgagtcc gacgcgcggg gcgccg
//
LOCUS       DASVOR010002266         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113183,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002266 DASVOR010000000
VERSION     DASVOR010002266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113183"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /locus_tag="VF516_41950"
     CDS             <1..820
                     /locus_tag="VF516_41950"
                     /inference="COORDINATES: protein motif:HMM:NF019344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8114388.1"
                     /translation="ARVIARFKAGLDDPQRPVGALLFAGPTGVGKTELAKQLARYLFG
                     DADRLVRVDMSELVTGPAITRLIAPSPGGASLADRIRRQPLSVVLFDEVEKADAAAFD
                     LLLGVIGDGRLTDALGRLVDFRMAVIVLTTNLGAADPRPAGFSADAEPVPDYGGALRR
                     FFRPELLGRLDAVVAFRPLAAAALERIVELELGKLRLRPGVIARNLQLVITPAARSRL
                     AQLGHDPRLGARPLRRTIEDLVVAPIAERIAREPTWRDTTVRITTSEEHGDIRV"
     gene            937..1626
                     /locus_tag="VF516_41955"
     CDS             937..1626
                     /locus_tag="VF516_41955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEX8114389.1"
                     /translation="MPDPKRVLWYLRKVPLLADLGPDAIARMAERVELREARRRDVVY
                     LPGDPGRSLFFVHGGRIKVSKVTRDGKSLTLAYHGPAELFGDSCLLDGGPRTEMAEAV
                     ENALLSEIERAHFEELIHAHPALGLGMTRLMIVRRRELENKVEALVFRDVSSKLAELL
                     VKLAGENGVDDARGTLIALKITHQELANLIGSTRETVSLTLSTFKRKRLIQTEGRKVI
                     ISDAEALRALY"
BASE COUNT          234 a          627 c          536 g          231 t
ORIGIN      
        1 cgcccgcgtg atcgcgcggt tcaaggccgg gctcgacgat ccgcagcggc cggtcggcgc
       61 gctgctgttc gcggggccca ccggcgtcgg caagaccgag ctcgccaagc agctcgcgcg
      121 ctacctgttc ggcgacgccg accggctggt ccgggtcgac atgagcgagc tggtcaccgg
      181 cccggcgatc acgcggctga tcgcgccgtc gccgggcggc gccagcctcg ccgaccggat
      241 ccgccgccag ccgctcagcg tcgtgctgtt cgacgaggtc gagaaggcgg acgccgccgc
      301 gttcgacctc ctgctcggcg tgatcggcga cggccggctg accgacgcgc tcggccggct
      361 ggtcgacttc cgcatggcgg tgatcgtgct gaccaccaac ctcggcgctg ccgacccgcg
      421 ccccgccgga ttctccgccg acgccgagcc ggtccccgac tacggcggcg cgctgcgccg
      481 gttcttccgc cccgagctgc tcggccggct cgacgccgtg gtcgcgttcc ggccgctcgc
      541 cgccgccgcc ctggagcgca tcgtcgagct cgagctcggc aagctccggc tccgccccgg
      601 cgtgatcgcg cgcaacctcc agctcgtgat cacccccgcc gcgcgctccc ggctcgccca
      661 gctcggccac gacccgcgcc tgggcgcacg cccgctgcgc cgcacgatcg aggatctcgt
      721 cgtcgctccg atcgccgagc ggatcgcgcg cgagcccacc tggcgcgaca ccaccgtgcg
      781 catcaccacc tccgaggaac acggggacat ccgcgtgtga cggacccggg atcttccgag
      841 gcataaacta ctagaattat tcactcgccg cggtgcgcta ttggtaatct gcgcgacacg
      901 cacccagtcg tttgcaagca ttgctacaat tagaacgtgc ccgaccccaa gcgcgtactc
      961 tggtacctcc gcaaggtacc gctgctggct gacctcggac cggatgcgat cgcgcggatg
     1021 gccgagcgag tcgagctccg cgaggcccgc cggcgcgacg tggtctacct gcccggcgac
     1081 cccggccgct cgctgttctt cgtgcacggc ggccgcatca aggtgagcaa ggtcacccgc
     1141 gacggcaagt cgctcacgct cgcgtatcac ggcccggccg agctgttcgg cgacagctgc
     1201 ctgctcgacg gcggcccgcg caccgagatg gccgaggccg tcgagaacgc cctgctcagc
     1261 gagatcgagc gcgcgcactt cgaggagctg atccacgccc acccggcgct cggcctgggc
     1321 atgacccggc tcatgatcgt ccgccgccgc gagctcgaga acaaggtcga ggccctggtg
     1381 ttccgcgacg tgtcgagcaa gctcgccgag ctgctggtca agctcgccgg cgagaacggc
     1441 gtcgacgacg cgcgcggcac gctgatcgcg ctcaagatca cccaccagga gctcgccaac
     1501 ctgatcggct cgacccgcga gaccgtgtcg ctcacgctgt cgacgttcaa gcgcaagcgg
     1561 ctgatccaga ccgagggccg caaggtcatc atctcggacg ccgaggcgct gcgcgcgctg
     1621 tactagcc
//
LOCUS       DASVOR010002267         1975 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_170507,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002267 DASVOR010000000
VERSION     DASVOR010002267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170507"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..1225)
                     /locus_tag="VF516_41960"
     CDS             complement(179..1225)
                     /locus_tag="VF516_41960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114390.1"
                     /translation="MKRTRYDDVCEELVNSPRRWLVTGVAGFIGSALLERLLDLGQSV
                     VGVDNFLTGHKRNLEDVLAINPDERLQFRFIEGDLRDPEVAREACKDVDIVLHEAALG
                     SVPRSIKDPLASHTHNVDAFLNVLWAAKEAGVGRLVYASSSSVYGDHAGLPKHEDRIG
                     KPLSPYAATKRADEIYAQVFHDVYGQQLIGLRYFNVFGRRQDPDGPYAAVIPRWIANL
                     IAGKPCVIFGDGSNSRDFCYVDNIVQANLLAATVPDPAVTGTVYNCGCNGRTDLKQLF
                     AMIRDNLAKSVPEIAGAEPVFEAPRAGDVAHSQAAIDKISAALGYVPSHLVDDGMAET
                     VAWFAHSLQGLRRE"
     gene            complement(1222..>1975)
                     /locus_tag="VF516_41965"
     CDS             complement(1222..>1975)
                     /locus_tag="VF516_41965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HEX8114391.1"
                     /translation="NEVDALYASIVPAGTHKAPSIEVAEAAKVIENTQRDLNIALMNE
                     LALIFDRMGIRTADVLEAAGTKWNFLKFAPGLVGGRCIGVDPYYLTTKAEELGYLPEV
                     ILAGRRINNNIGPFLAQKCVKMLTHSDVALRKARVGILGLTFKENVPDLRNSKIPDIV
                     TELATFGIQAMVHDPLGNPDEAYHEYKIRLTPLEQLVDLDAVILAVAHKAYVSNAGAI
                     YERVRDNGVVIDVKSVLPAHVKPPRGIQLWSL"
BASE COUNT          315 a          643 c          666 g          351 t
ORIGIN      
        1 tgagctgcaa cgcgagcttc tcgtcgatcg ctttcggctc cggcttgggc aatgcatcgt
       61 ctgccatctt cgcgccgcga gcttaccaaa cttggctcgc actggacgcg ctgcgttgcg
      121 ctatcgcgcc tcgaaaaacc ggggctgaag ctcgctgcgc atcgcgatgc gaggctcgtt
      181 actcgcgccg cagcccctga agagagtgag caaaccacgc cacggtctcc gccattccgt
      241 cgtcgaccag gtgggatggg acgtacccga gcgctgcgct gatcttgtcg attgccgcct
      301 gcgagtgagc gacgtcgccg gcccgcggcg cctcgaacac cggctcggca ccggcgatct
      361 cgggaacgct cttggcaaga ttgtcccgga tcatcgcgaa cagctgcttg aggtccgtgc
      421 ggccgttgca gccgcagttg tacaccgtgc cggtgacggc aggatcgggc accgtcgccg
      481 ccagcaggtt ggcctggacg atattgtcga cgtagcagaa gtcccggctg ttcgagccat
      541 cgccgaagat cacgcacggc ttgccggcga tcagatttgc gatccagcgc gggatcaccg
      601 cggcgtaggg cccgtcggga tcctggcggc gaccgaacac gttgaagtag cgcagcccga
      661 tcagctgctg gccgtagacg tcgtggaaca cctgggcgta gatctcgtcg gcgcgcttgg
      721 tcgcggcgta cggcgacagc ggcttgccga tccgatcctc gtgcttgggc agccccgcgt
      781 gatcgccgta gaccgacgac gacgacgcgt agaccaggcg gccgaccccg gcctccttgg
      841 ccgcccacag cacgttgagg aacgcgtcga cgttgtgggt gtgcgaggcc agcgggtcct
      901 tgatcgaccg cggcaccgag ccgagcgcgg cctcgtgcag gacgatgtcg acgtccttgc
      961 acgcctcgcg cgcgacctcc ggatcgcgga ggtcgccctc gatgaagcgg aactgcagcc
     1021 gctcgtcggg gttgatcgcg agcacgtcct cgaggttgcg cttgtggccg gtcaggaagt
     1081 tgtcgacccc gaccacgctc tggccgaggt cgagcagccg ctccagcagg gccgatccga
     1141 tgaaccccgc gaccccggtc accagccagc ggcgcggcga gttgaccagc tcctcacaga
     1201 cgtcgtcgta gcgtgtgcgc ttcatagact ccagagctgg atcccccgcg gtggcttgac
     1261 gtgcgccggc agcacgctct tgacgtcgat cacgaccccg ttgtcgcgca cccgctcgta
     1321 gatcgcgccg gcgttcgaga cgtaggcctt gtgggcgacc gcgaggatca cggcgtcgag
     1381 gtcgaccaat tgctcgagcg gggtcagccg gatcttgtac tcgtgatagg cctcgtcggg
     1441 attgcccagc ggatcgtgga ccatcgcctg gatcccgaac gtcgcgagct cggtcacgat
     1501 gtcggggatc ttggagttgc gcaggtcggg aacgttctcc ttgaaggtca ggccgagaat
     1561 gccgacccgc gccttcctga gcgcgacgtc ggagtgggtc aacatcttga cgcacttctg
     1621 ggcgaggaac ggaccgatgt tgttgttgat ccgccggccg gcgaggatga cctcgggcag
     1681 gtagccgagc tcctcggcct tggtcgtcag gtaatacggg tcgacgccga tgcagcggcc
     1741 gccgaccagg ccgggggcga acttgaggaa gttccacttg gtgcccgccg cctccagcac
     1801 gtcggcggtc cggatcccca tgcgatcgaa gatcagcgcg agctcgttca tcagcgcgat
     1861 gttgagatcg cgctgggtgt tctcgatcac cttggcggcc tcagccactt cgatcgaagg
     1921 cgccttgtgc gtgccggccg ggacgatcga ggcatagagg gcatccacct cattg
//
LOCUS       DASVOR010002268         1985 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_462741,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002268 DASVOR010000000
VERSION     DASVOR010002268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1985
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462741"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VF516_41970"
     CDS             complement(<1..704)
                     /locus_tag="VF516_41970"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114392.1"
                     /translation="MLGIVIGNYQIIRKLGEGGMGAVYLGQHQLLGRRAAIKVLLPEL
                     SARPDIVNRFFNEARAVTSISDPGIVQVFDFGYHTDGSAFIVMEYLEGEPLDRRLARL
                     GKLPAYEALLLCRQIASSLAAAHAQNIIHRDLKPENIYLVHDGEVASGERSKILDFGI
                     AKLSDDNPGKIKTSTGALMGTPIYMSPEQCRGLATLDHRSDIYSLGCVLFHLLTGRPP
                     FEGEGMGDIIAAHIREP"
BASE COUNT          331 a          701 c          653 g          300 t
ORIGIN      
        1 ggctcgcgga tgtgcgccgc gatgatgtcg cccatgccct cgccctcgaa cggcggccgc
       61 ccggtcagca ggtggaacag cacgcagccc agcgagtaga tgtccgagcg gtggtcgagc
      121 gtcgccagcc cgcggcactg ctcgggcgac atgtagatcg gcgtgcccat cagcgcgccg
      181 gtgctggtct tgatcttccc cgggttgtcg tcggacagct tcgcgatccc gaagtcgagg
      241 atcttcgagc gctcgcccga ggcgacctcg ccgtcgtgga ccaggtagat gttctccggc
      301 ttgagatcgc ggtggatgat gttctgggcg tgcgcggcgg cgagcgagct cgcgatctgg
      361 cggcacagga gcagcgcctc gtacgccggg agcttgccca gccgcgcgag ccggcggtcg
      421 agcggctcgc cctcgaggta ctccatgacg atgaacgcgc tgccgtcggt gtggtagccg
      481 aagtcgaaga cctgcacgat ccccggatcg ctgatcgacg tcaccgcgcg ggcttcgttg
      541 aagaaccggt tcacgatgtc gggccgcgcg ctcagctcgg gcaagaggac cttgatcgcg
      601 gcgcgccgcc cgagcagctg gtgctgcccg aggtacaccg cacccatccc gccctccccg
      661 agcttgcgga tgatctggta gttccctatg acgatgccga gcaacgatgg ccttcacttg
      721 ctgcccggcg cgcgccgggc cacctgtctg gcaatacgta ccggttcccc gatcttccgg
      781 atgcgcaccg ccctaacgcg aatagcaacg ccgggaccat cgccacgccg cgcgggcgac
      841 ctcatcgctt cgcgaccttg gcgctcgcag cgatgacacg tggcgacacg tgacagatcc
      901 ggggtgacgt gcaatccagg tgagcagcgc tccagatcct gccagcacgg gcgggcaccc
      961 tgcgggtgta acacatcgga gatccagcaa gtgcgtcctc atggggcccc tccgtgcatc
     1021 tccgcggtgt ccacttcact ggacaccgcg aggtcgcggc gtccatcccg caggacgtcg
     1081 gtgtgccgtg tcgcacctca cgtacgccca cgacagaccc gtcgccgctg tgcccggtgc
     1141 cacgaaacat gtctcccgtt ccgggtctca ccgtggaaca ggggcgattc gctcgcccgg
     1201 ggccttcccg atcggtcgac aatgttccga cccagtatcg gggatccagt cgctcgccac
     1261 caaggcctca gagcaggcga gcgcctcgct acgcttcgcg aacgcgcagg tcaccgcgct
     1321 ctcggcgcag atggaggcac gccggaaggc ggccggtcgc tgaatcccgg aaacagacgc
     1381 acccgacgac ccgccggccg ctaccgcggg caacgtcccg gtcatcgggt tcgcacgtga
     1441 acgccgcccg ggaagtcatc atcggtcaag cccgcggccc atgcatcgga caaagaccgg
     1501 cgctctcccg actcctacgc tgccgtcatg gccaagcccg ccagccgcag aagggcagcc
     1561 gcgcccgccg gaccggcttc tccggaaccc aagggataaa aatcccggaa atggagaata
     1621 cgcgcggggc accggattgt gagcaggcgg tcgaggtcaa cctggtcaat cgactccggc
     1681 tggtcacaag ccaccctggc gatctcccga ggtcctccgc tgccgtcgcg acgaagcccg
     1741 ctcgccgcgg agatcagcca ggccgcagcg cgtggtcgag cgcgaggacg tgcaccagcg
     1801 ttccccggtc gcaaggagca ggacgacttg atcagatgct gcaactcatc gattgagcag
     1861 cgactgaggc tgactaccgg ccggcgagca gcgcgttcat gtcgcggtgg gtgatcgcac
     1921 cgtcggacgc ctcgcacgcg ccatcgcgca ggaacccggt cgcgagcgcc cgggcccggg
     1981 tcagc
//
LOCUS       DASVOR010002269         4229 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113218,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002269 DASVOR010000000
VERSION     DASVOR010002269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113218"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            775..4059
                     /locus_tag="VF516_41975"
     CDS             775..4059
                     /locus_tag="VF516_41975"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8114393.1"
                     /translation="MAPQLRDHERYDILGEHGRGGLGRVSRAHDRELGRDIAIKELIA
                     RGHLSEVRFLREALITARLEHPGIVPVYEAGRWPDGTPFYAMKLVSGRPLRDLIAERK
                     TVDERLGLLHHVIAVADAIAYAHGRSIIHRDLKPANVIVGEFGETVVIDWGLAKDLTA
                     AEEAPIGGGPFRDRRDDGLTSAGAVLGTPAYMAPEQARGEPVDQRADVFAIGAMLWEL
                     CSLHKLPAGSSGQRRRALRRAGIDHDLIAIIEKALAPDREQRYPEAGALAADLKAFKS
                     GARIAARSYSLFGMLAHWTRRHRALAGSVAAAVVVAIGASLFYVRSVATERDRADAAL
                     ARVEATNDKLARERAAAIGQRNELILSQATAALESDPTRAIEWLETYPPDGAHWDRVQ
                     VIAADAQSRGVARHILPSTSWTISISPDGKTVMTAGMHDLVQVWDLATGRRLHGLSLG
                     GEVAAAAFAPDGHAIAIGGQGGEVVLWSPISDARTRLGKLDSGALSVAFSPDGQLVIA
                     ASLHAVAAWDVTALRQVWSMPSAQAVTFLAVAPSGRTLAFGTADGTLHVSDFDPGHTR
                     QLHGHRGPISWIAFSAEGRSIATGSVDHSVRWWDPVTGAGHLLGMHDGQVTEVRFSPV
                     DPTLVASGGTDQKLRLWSTVGGGARVFSGHTDKVLGVMFAPDGQTVASVSSDRQVRLW
                     DVASGDSRVLSGHLGDVGDVRFSPDGSLLVSSGFDPGTRVWRLTPPPGRTLGRHEYEA
                     LQAVFSPTGRFVASTGADRTARLWDLASGRERSLPGSGYTAHALPTGVMFSTRGELLA
                     SVGDSAAHLWNLSTGEARVFVAPDALLRRVAITGDGRLLAAGDVEGGIRVWEVASNRL
                     TTLAGHTDEVTELAFLPRGDRLVSAAMDGTIRIWDVSSSATTGQVIGTHRSAITRLAV
                     SPAGNLVASGGRDGTVHLWNLTTHADRALAGHTGSIAGLVFSRDGRRLASASLDATVR
                     IWNVDTGEARVLRGHDEEVRTVAFAPDGHVVASGGNDRTVRLWNLETGAIAIVRGHHA
                     IVTSVAFDADGEQLVTTSEDATVRLWDLDRITWAPRDRGELTAWLQAQTTAALPPPRI
                     DESSSH"
BASE COUNT          598 a         1504 c         1506 g          621 t
ORIGIN      
        1 ccaggatctc gttgttccac cgcatgtccc tgacgacgcc cggaacatcg agggcttgcg
       61 cttcgatcca ggacatccgg agaaccaagg gccgctgctc cgggccgctg ctccgggcca
      121 ctgctccgga tgaatcgaag gattgcgcgc aggcccaccg cgaccggaac ccgccaggcg
      181 ccgcgcgagc cggcgagcac ctggaccgag cggatcgtcc ggagccgctg agctctcgct
      241 gtctccgccg atcgtccccc cgacgcgcgg cgcgtcgagc gctcgggttc ggccgcggaa
      301 cacgcagaac actgttagag ctccgcctga tcttcctgat tccaagttgt tagcaatcat
      361 cggaacagac cggaaccgca tccagaacat ctggacaccc tgcgttccgg ttccgatgaa
      421 acagccacca gcacttcgcc gtgctcgggg ctcggcccgg cgcttgctct gtcgccgggc
      481 gtgccttgga gttcggggga agtggaacgg gcccgccgcg cgggcggttc gccgatcgtg
      541 cgcacccccg gaacgtcggg cgataacgag gccctcgcag agacccaacc cgcggcgtca
      601 tcgtgcgttg ccccggcggc acctgccgga cccggctctc gcgcggcgga tccaaccggt
      661 ccgtcgccgg ccgacccgac gcgcaccagc cagccggacc ggccctccga ccccacgcgc
      721 accagcgagc ccgatcggcc cgccgcatcg ccatcccgtg cgccgcgtcc cccgatggcc
      781 ccgcagctcc gcgaccacga gcgctacgac atcctgggcg agcacggccg cggcggcctg
      841 ggccgcgtat cccgcgcgca cgaccgcgag ctcggccgcg acatcgcgat caaggagctg
      901 atcgcgcgcg gccatctcag cgaggtccgc ttcctgcgcg aggccctgat caccgcccgc
      961 ctcgagcacc ccggcatcgt cccggtctac gaggccggcc ggtggcccga cggcaccccg
     1021 ttctacgcca tgaagctggt gtccggccgc ccgctgcgcg acctgatcgc cgagcgcaag
     1081 accgtcgacg agcggctcgg cctgctccac cacgtgatcg cggtcgccga cgcgatcgcc
     1141 tacgcccatg gcaggagcat catccaccgc gacctcaagc ccgccaacgt catcgtcggc
     1201 gagttcggcg agaccgtggt catcgactgg ggcctggcca aggacctcac cgccgccgag
     1261 gaggccccga tcggcggcgg cccgttccgc gatcgccgcg atgacgggtt gacctcggcc
     1321 ggcgcggtcc tcggcacccc ggcgtacatg gcgcccgagc aggcgcgcgg cgaaccggtc
     1381 gaccagcgcg ccgacgtgtt cgcgatcggc gccatgctgt gggagctgtg ctcgctgcac
     1441 aagctgccgg ccggctcctc cggccagcgc cggcgcgcgc tgcgccgggc cgggatcgac
     1501 cacgatctga tcgcgatcat cgagaaggcg ctcgcgcctg atcgcgagca gcgctatccc
     1561 gaagcgggag cgctggcggc ggatctgaag gcgttcaagt cgggcgcgcg gatcgcggcg
     1621 cggagctact cgctgttcgg gatgctcgcg cactggacgc gccggcatcg cgcgctggcc
     1681 ggatcggtgg cggccgcggt ggtcgtggcg atcggcgcga gcctgttcta cgtgcgcagc
     1741 gtcgcgaccg agcgggatcg cgccgatgcg gcgctggcgc gcgtcgaggc gacgaatgac
     1801 aagctcgccc gcgagcgcgc ggccgccatc ggacagcgca acgagctgat cctgtcccag
     1861 gcgactgcag cgctcgagtc cgatccgacg cgcgcgatcg agtggctcga gacgtacccg
     1921 ccggacggtg cccactggga ccgcgtgcag gtgatcgctg cggatgcgca gagccgcggg
     1981 gttgcgcgtc acatcctgcc ctcgacgtcg tggacgatct cgatctcgcc ggacggcaag
     2041 accgtcatga ccgcggggat gcatgacctg gtgcaggtgt gggatctggc cacgggtcgc
     2101 cgcctgcacg gcctgtcgct gggcggagag gtggccgcgg cggcgttcgc gcccgacggc
     2161 catgcgatcg cgatcggcgg tcagggcggt gaggtcgtgc tgtggtcgcc gatctccgat
     2221 gcgcgcacgc ggctcggcaa gctggacagc ggcgcgctgt cggtcgcgtt ctcgcccgat
     2281 ggccagctgg tcatcgcggc gagcctgcac gcggtcgccg cgtgggatgt gacggcgttg
     2341 cgccaggtgt ggtcgatgcc gtcggcgcag gcggtgacgt ttctcgccgt ggccccgtcc
     2401 ggaaggacgc ttgccttcgg cacggcggac ggcacgctcc acgtgtcgga cttcgaccct
     2461 ggacacacgc ggcagctcca tggacaccgc ggtccgatct cgtggatcgc gttctcggca
     2521 gaggggcgct cgatcgcgac cggcagcgtg gatcactcgg tgaggtggtg ggatccggtc
     2581 accggcgccg gccacctgct gggcatgcac gatggccagg tgaccgaggt ccggttctcc
     2641 ccggtggacc ccacgctggt cgcatcgggt ggtaccgacc agaagctgcg actgtggagc
     2701 accgtgggtg gcggcgctcg cgtgttcagc gggcacaccg acaaggtcct cggggtgatg
     2761 tttgcgcccg acggacagac cgtggcgtcg gtatcttccg accggcaggt gcggctgtgg
     2821 gacgtggcat cgggcgactc ccgcgtcctg agcggccacc tcggcgatgt cggtgacgtg
     2881 cggttctcgc cggacggctc gctgctcgtg tcgtccggct tcgatccagg gacgcgggtc
     2941 tggcgtctca cgccgccacc gggtcgcacg ctcggtcgcc acgagtacga ggcgctgcag
     3001 gccgtgttct cgccgaccgg ccggttcgtg gcgtcgaccg gcgcggaccg aaccgcgcgg
     3061 ctgtgggatc tggcgtccgg ccgcgagcgg agcttgccag gctccgggta caccgcgcac
     3121 gcgctcccga cgggggtgat gttctcgacc cgcggtgagc tgctggcatc ggtcggtgac
     3181 agcgccgctc atctgtggaa cctctcgacc ggcgaagccc gcgtgttcgt cgcgcccgat
     3241 gcgctgctcc gcagggtcgc gatcaccggc gatggccgcc tgctcgccgc gggagatgtc
     3301 gagggcggga ttcgcgtgtg ggaggtggcg tccaaccgtc tcaccacgct cgcggggcac
     3361 accgacgagg tcaccgagct cgcgtttctg cctcgcggcg accgcctggt ctccgccgcg
     3421 atggacggca cgatccggat ctgggacgtg tcgtcctcgg cgacgaccgg tcaggtcatc
     3481 ggcacgcaca gatctgcgat cacgaggctt gcggtctcgc ccgcggggaa cctcgtcgcg
     3541 tcgggcggcc gcgacggcac ggttcatctg tggaacctca ccacccacgc ggaccgagcg
     3601 ctcgccggcc acaccggcag catcgcgggc ctcgtgttct cgcgcgatgg ccgccggctg
     3661 gcatcggcga gcctggacgc gaccgtgcgg atctggaatg tcgacaccgg cgaggcgcgt
     3721 gtgctgcgag gccacgacga ggaggtgcgc accgtggcgt tcgctcccga tggccacgtg
     3781 gtcgcgagcg gtgggaatga ccgcaccgtc cggttgtgga acctcgagac cggagcgatc
     3841 gcgatcgtgc gtggccacca tgccatcgtg acctcggtcg cgttcgacgc cgacggcgag
     3901 cagctggtga cgaccagcga ggacgcgacg gtgcggctat gggacctcga tcggatcacg
     3961 tgggcgccgc gcgaccgcgg cgagctcaca gcgtggcttc aggcgcagac gaccgcagca
     4021 ttgccgccac cccggatcga tgaatcatcg agccattgac catttcccag gaggtagata
     4081 cgtgcagcag agcagtgacc cgaaggaatc gagcaagaag aagctcgtcc ttcgcaagga
     4141 gtccatccgg gccctcgacg gagtgaacct ggcactggtg gccggcggcg ccgataccgc
     4201 ggccacctgc aatcccccgc ccaatcgcc
//
LOCUS       DASVOR010002270         2825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_286427,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002270 DASVOR010000000
VERSION     DASVOR010002270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286427"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="VF516_41980"
     CDS             complement(<1..863)
                     /locus_tag="VF516_41980"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114394.1"
                     /translation="MARTTDDADPTLPPELGAGSGAAFAATIAGGPTAPPTPPDGPVP
                     TASAVVTRPVPSALDAAATLDAPLPGEPSLLPYVPMTNYRPEREIARGGMGRIVAAED
                     LRLGRAVALKELIDPVAEQRGRFQREALITARLQHPGIVPVYEAGRWPSGDPFFAMKL
                     VSGRPLDRVIADAATLADRLALLPRLAAAADAIAYAHSQRVIHRDLKPANVLIGEFGE
                     TVVIDWGLAKSLDDVDAPESLRPAARPVRTATPAGSAGAGTVRTDEHTASTLTVAGSV
                     MGTPAYRAPEQA"
     gene            885..1646
                     /locus_tag="VF516_41985"
     CDS             885..1646
                     /locus_tag="VF516_41985"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8114395.1"
                     /translation="MSSGLHVVRAGAGPQILLIHGSAADHRTWSILLASPLRARFTLV
                     AYDRRAGVESIDDHVADAAALLGPERTVVVGSSFGAVIALELVRRCGDRIAGAVLIEP
                     PLNSSDDAPAVRAEFLDDFDRRAAEAGGPAAGELFLRTVLGDDAFERMPRVFQTQAMA
                     KFAEIRADSVALFAYRPRYAELAAVRVPVLLLGGERSARWYRRTLDELYRALPGARLE
                     IVPGAGHMLHADASRRFHALLEQFAAPLLGAAAPA"
     gene            1916..>2825
                     /locus_tag="VF516_41990"
     CDS             1916..>2825
                     /locus_tag="VF516_41990"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114396.1"
                     /translation="MRPGERLEDRFEIEQPIGSGGMGTVFRAHDPISRGAVAVKVISG
                     DQGHVTGRFAREVKVLAELSHPGIVRYISHGTTPSGELFLVMEWLDGETLKSRLQRGP
                     LSASDAVVLATRVAGALGAAHALGIVHRDLKPSNLFLPGGRVDQVKLLDFGIAQREGK
                     TQLTHSGVMLGTLGYMAPEQARASGLIDARADVFALGCVVFQCLTGAPAFDGDTAAAI
                     LAKILFDPAPRVSELWPGVPENLDALVAQMLSKDPALRPSDGANLAAALAALGPLTQT
                     TGPRERPARARASTTGERRLLAVVMLG"
BASE COUNT          343 a         1046 c         1044 g          392 t
ORIGIN      
        1 gcctgctcgg gcgccctgta cgccggcgtg cccatcaccg agccggcgac ggtcagcgtc
       61 gacgcggtgt gctcgtcggt ccggaccgtg ccggcaccgg cggatccggc cggggtcgcc
      121 gtccggaccg ggcgcgccgc cgggcgcagc gactccggcg cgtcgacgtc gtcgaggctc
      181 ttggccagcc cccagtcgat caccacggtc tcgccgaact cgccgatcag cacgttggcc
      241 ggcttgaggt cgcggtggat cacgcgctgg ctgtgcgcgt aggcgatcgc gtcggccgcg
      301 gcggcgagcc ggggcaacag cgcgagccgg tcggccagcg tcgcggcgtc ggcgatcacg
      361 cgatcgagcg ggcgccccga caccagcttc atcgcgaaga acgggtcgcc cgacggccag
      421 cggccggcct cgtacaccgg cacgatcccc gggtgctgca gccgcgcggt gatcagcgcc
      481 tcgcgctgga accggccgcg ctgctcggcg accggatcga tcagctcctt gagcgcgacc
      541 gcgcggccga gccggaggtc ctcggcggcg acgatccggc ccatcccgcc gcgcgcgatc
      601 tcgcgctcgg gccggtagtt ggtcatcggc acgtagggca agagcgacgg ctcgccgggg
      661 agcggcgcgt cgagcgtggc cgcggcgtcc agcgccgacg gcaccggccg ggtcaccacc
      721 gccgaggccg tcgggaccgg accgtcgggc ggcgtcgggg gcgcggtcgg tccccccgcg
      781 atcgtcgcgg cgaacgcggc acccgagccc gctcccagct ccggaggaag ggtcgggtct
      841 gcgtcgtccg tggttcgggc catgtatgct catcatactc cgtcatgtcg tccgggctcc
      901 acgtcgtacg cgccggtgcc ggcccccaga tcctcctcat tcacggctcg gccgccgacc
      961 accggacctg gtcgatcctg ctcgcgtcgc cgctgcgggc gcggttcacg ctcgtcgcgt
     1021 acgaccgccg ggccggcgtg gagtcgatcg acgaccacgt cgccgacgcc gccgcgctgc
     1081 tcggccccga gcgcaccgtc gtcgtcggct ccagcttcgg cgcggtgatc gcgctcgagc
     1141 tggtccgccg ctgcggcgac cggatcgccg gcgcggtcct gatcgagccg ccgctgaaca
     1201 gctccgacga cgcgcccgcg gtccgcgcgg agttcctcga cgacttcgat cgccgcgccg
     1261 cggaggccgg cggtccggcc gccggggagc tgttcctgcg caccgtgctc ggcgacgacg
     1321 cgttcgagcg catgccgcgc gtgttccaga cccaggcgat ggcgaagttc gccgagatcc
     1381 gcgccgactc ggtcgcgctg ttcgcgtacc gcccgcgcta cgccgagctc gccgctgtcc
     1441 gcgtgccggt gctgctgctc ggcggcgagc gctcggcgcg ctggtatcga cgcacgctgg
     1501 acgagctgta tcgcgcacta cccggtgctc ggctggagat cgtccccggc gccggccata
     1561 tgctccacgc cgatgcgtcg cgtcggttcc acgcgctgct cgaacaattc gcggcgccgc
     1621 tgctcggcgc ggccgcgccg gcgtgacgca ccgacgctcg gcccgtggcg caaacgcgtg
     1681 cgtggtgggc cgacaccgac atgcacggca aagtcggtca gcgtcgggct gagtcgagcg
     1741 caccgtcgcg agcgccggcg caccggagtc tcgcttgccg ggccgctgtc gggtgatagc
     1801 agggtgcgct ccttgtgagc ccgggcattc catgcaacat ggcgagtgtg tcgagcgctc
     1861 ggagtcctgc cgtggcgcat gcgctgggcg gccagccggc gatagaggtc tgcccgtgag
     1921 accgggagag cggctcgagg atcgattcga gatcgaacag ccgatcggca gcggcgggat
     1981 gggaacggtg tttcgcgcgc acgacccgat ctccagggga gcggtggccg tcaaggtgat
     2041 ctccggagac cagggccacg tcaccggccg gttcgcgcgc gaggtcaagg tgctcgcgga
     2101 gctctcgcac cccggcatcg tccgctacat ctcgcacggc acgacgccgt cgggggagct
     2161 gttcctcgtc atggagtggc tcgacggcga gactctcaag tcgcggctcc agcgcggccc
     2221 gctctccgcg agcgatgcgg tcgtgctcgc gacccgggtc gccggggcgc tcggcgccgc
     2281 ccacgcgctc ggcatcgtcc accgcgatct caagccgtcc aacctgttcc tccccggcgg
     2341 ccgcgtcgac caggtcaagc tgctcgactt cgggatcgct cagcgcgagg gcaagaccca
     2401 gctcacgcac agcggggtga tgctcggcac gctcgggtac atggcgcccg agcaggcgcg
     2461 ggcgagcgga ctgatcgacg cgcgcgccga cgtgttcgcg ctcggctgcg tggtgttcca
     2521 gtgcctgacc ggcgcgccgg cgttcgacgg cgacaccgcg gccgcgatcc tggccaagat
     2581 cctgttcgac cccgcgccgc gggtcagcga gctgtggccc ggggtgcccg agaacctcga
     2641 cgcgctggtc gcgcagatgc tgtcgaagga tcccgcgctg cggccgagcg acggcgccaa
     2701 cctggccgcc gcactggcgg cgctcggtcc gctgacccag accacgggcc cgagggagcg
     2761 cccggcgcgc gcgcgagcga gcaccaccgg cgagcggcgg ctgctggccg tcgtgatgct
     2821 gggtc
//
LOCUS       DASVOR010002271         5122 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_551169,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002271 DASVOR010000000
VERSION     DASVOR010002271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_551169"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..596)
                     /locus_tag="VF516_41995"
     CDS             complement(<1..596)
                     /locus_tag="VF516_41995"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114397.1"
                     /translation="MDQLGAGGMGVVYVGHHEALGRPVVMKVLQPELCSDAGRVQRLF
                     DEARAVTAVRSPGIVQMFDFGVTPDHRAYFVMELLEGESLAARLQQRRLDHAACCRLG
                     RQVANVLQAAHAADIIHGDLKPANLFLVPDPEIVGGERVKVLDFGIAKLTGEARSAGA
                     RTRAGLMMRTLHYMSPEQCGSASTADVRSDIYALGCIL"
     gene            882..1847
                     /locus_tag="VF516_42000"
     CDS             882..1847
                     /locus_tag="VF516_42000"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8114398.1"
                     /translation="MNRRTLLKAAAAAGSALALPPARAQPPAPPAAAKGPPVWLDLDQ
                     KALDDAYDQSVWATNGKQVLDRYAVLSEDVRTRLGMPQRMSYGTTPVEALDVYRARSG
                     APIQIFLHGGAWRSGTAKEWAFPADMFVHRGAHFVVPDFVSVVDAGGKLSAMAEQVRS
                     AIAWTYRNAGRFGGDRERIFLSGHSSGAHLAGVALTTDWKRLGLPSDLVKGGVLLSGM
                     YDLKPVRLSARSRYVHFDDATEEALSPQRHLERLPARLAVAYASLDSPEFQRQSRDFA
                     AAVKAAGRPVELLVARGYNHFEAPETLGNPYGLLGRAALAQMGLG"
     gene            complement(1876..2880)
                     /locus_tag="VF516_42005"
     CDS             complement(1876..2880)
                     /locus_tag="VF516_42005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEX8114399.1"
                     /translation="MRVLRRRLITLGLVGFASVAPRARAAPPPLSAVQVIPLPGVERR
                     IDHFAMDPAGKRLFVAALGHGTLEVLDIGAGTRITSIAGLKEPQGVAYLPVLHRIVVA
                     TRGGAVAAFDDVTYRRTATLPDLGDADNLRYDAAAGQLYLGYGEGALGVIEPSSLKLV
                     ASIPVGGHPESFRLEESGPRAFVNVPATREVLVVDRRQRKAVMHIPLGGPSNNYPMSL
                     DETGHRLFVGVRRPAQLLVFDTVTGKRLAAVPCVGDTDDLFYDARRDRVYVVGGEGYV
                     DIFDAAASGKYDRLARIATRAGARTGLWSTEIDRLFVAWPSRDGHPAEIHVLAPPGGP
                     "
     gene            3094..4002
                     /locus_tag="VF516_42010"
     CDS             3094..4002
                     /locus_tag="VF516_42010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114400.1"
                     /translation="MPGKPSRFDYQWVDAAQRRLFIAHLGDSSLLVFDLDSQRVIQEV
                     PHLPSIHGVVAAPAQHLVLATATADTTRALIDDRTFQVTARVPAGEYPNGLAFDPASG
                     KVFVSNNTGRGVAVVDVAAARALPGIDIGGGAGNTQWDAASGHVLAAVHGSPSLVDID
                     PATSRIAGRIALHGVTTCHGLLVASTPGLAFAACRGAAPVLAVVDVRARRQTMTLPLP
                     AGIDVLAFDPGLQRLYAASETGTVAVFAVAADRSVTEMGHGFLGPNAHSVAVDPATHR
                     VYFPLENVGGHPVLRVMDARVATGSP"
     gene            complement(4055..>5122)
                     /locus_tag="VF516_42015"
     CDS             complement(4055..>5122)
                     /locus_tag="VF516_42015"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase family protein"
                     /protein_id="HEX8114401.1"
                     /translation="VARRRATEIVFAGRGVSARRHDRRAHVEQLAQLGVPVLATPADL
                     AEALGLSIPQLRWLCFHAEAPQKTHYVYFEVPKRSGGTRLLAAPHARLAAAQRWVLAH
                     VLARLEVTPHAHGFVTGRSTISNARLHVGKDIVVNLDLAEFFPTITFPRVRGLFESIG
                     YSPAVATLLALLCTESPRSAVVHDGVTFWVAAGDRALPQGACTSPAISNLVTRKLDRR
                     LAGAAGKLGWTYTRYADDLTFSTGTAAAGSLALLLARVRHIVRDEGFAVNERKGRVQR
                     RSQRQSVTGVVVNDRPGVPREELRRLRAILHRARTTGLAAQNRDHHPDFAAYLRGKIA
                     YIAMIDPARAAVLASALDALT"
BASE COUNT          727 a         1829 c         1857 g          709 t
ORIGIN      
        1 aacaggatgc agcccagcgc gtagatgtcg gagcgcacat cggcggtgct cgcgctgccg
       61 cactgctcgg gcgacatgta gtggagcgtg cgcatcatca ggcccgcgcg agtccgggcg
      121 ccggcggacc gggcctcgcc ggtgagcttg gcgatgccga aatcgagcac cttgacgcgc
      181 tcgccaccga cgatctcggg gtcggggacc aggaacaggt tggccggctt cagatcgccg
      241 tggatgatgt cggcggcgtg cgccgcctgc aggacgttgg cgacctggcg gcccagccgg
      301 cagcacgccg cgtggtcgag ccggcgctgc tggagacgcg cggcgagcga ctcgccctcc
      361 agcagctcca tcacgaagta tgcgcggtgg tcgggggtga cgccgaagtc gaacatctgc
      421 acgatgccgg ggctgcggac cgccgtcacc gcgcgcgcct cgtcgaacaa ccgctggacc
      481 ctgccggcgt cgctgcacag ctcgggctgc aacaccttca tgaccacggg gcggccgagc
      541 gcctcgtgat ggccgacgta gacgacgccc atgccgcctg caccgagctg atccacgact
      601 cgatagttgc cgagcaatgt ccccagcaca gcaatgtcac actccacggc tggccacgga
      661 ctgtcaagcg aggccaccgt gatgctcgtc ggagtacatc ggctcctcgc gagccgctgc
      721 gatgcgctcg tcgccgggtt acagcagtcc ggtaatcggc tgactcatcc gtgattccct
      781 ccagcgcgat gggaatcgcg gggacgccga tgtgcgagcc agtgtgcggc cgggtgaccg
      841 ccggtacggg gcgcaggcgt gatagatccg cggacacttc gatgaaccgc agaacgttgc
      901 tgaaagccgc cgcagccgcc ggcagcgccc tcgcccttcc gcctgcccgc gcgcagccgc
      961 cggcgccacc cgccgctgcg aagggcccgc cggtgtggct cgacctcgac cagaaggcgc
     1021 tggacgacgc gtacgaccag agcgtctggg cgaccaacgg gaagcaggtg ctcgaccgct
     1081 acgccgtgct gagcgaggac gtgcgcacgc gcctcggcat gccgcaacgc atgtcctacg
     1141 ggaccacgcc ggtcgaggcg ctggacgtgt atcgagcccg cagcggtgcg ccgatccaga
     1201 tcttcctcca cggcggcgcc tggcgctcgg ggaccgcgaa ggagtgggcg ttccccgcgg
     1261 acatgttcgt gcaccgcggg gcgcacttcg tggtgcccga tttcgtgtcg gtggtcgacg
     1321 cgggcggcaa gctgtccgcg atggccgagc aggtgcgaag cgcgatcgcc tggacctacc
     1381 gcaacgccgg gcggttcggc ggtgaccgtg agcggatctt cctgtccggc cactcgtccg
     1441 gcgcgcatct cgccggcgtc gcgctcacga ccgactggaa gcgcctcggg ctgccctccg
     1501 atctcgtgaa gggaggcgtc ctgctgagcg gcatgtacga cctgaagccg gtgcgcctgt
     1561 ccgcgcgcag ccgctacgtc cacttcgacg acgccaccga ggaggcattg agtccccagc
     1621 gccacctcga gcggctgcct gcacggctgg ccgtggccta cgcctcgctc gacagccccg
     1681 agttccagcg ccagtcgcgc gacttcgccg cggcggtgaa agccgccggc aggcccgtcg
     1741 agcttctggt cgcgcgcggc tacaaccact tcgaggcgcc ggagaccctg gggaatccct
     1801 acgggctcct gggccgcgcc gcgctcgccc agatggggct cgggtgacgg cgaggcgacg
     1861 tcccgctgca tctctctacg ggccgccggg cggggcgagc acgtggatct ctgccgggtg
     1921 gccgtcgcgg ctcggccagg caacgaacag ccgatcgatc tcggtggacc acagaccggt
     1981 ccgggctccg gcgcgggtcg cgatccgcgc gagacggtcg tactttccgg acgcggcggc
     2041 gtcgaagatg tccacgtagc cttcgccacc gacgacgtac acccgatcgc ggcgggcgtc
     2101 gtagaacagg tcatcggtgt cacccacgca gggcaccgcg gcgagccgct tgcccgtgac
     2161 ggtgtcgaac accaggagct gcgccggccg cctcacgccg acgaacaggc ggtgtccggt
     2221 ctcgtcgaga gacatcgggt agttgttcga cggcccgccg agcgggatgt gcatcacggc
     2281 ctttcgctgg cggcgatcca cgaccaggac ctctcgcgtg gccggcacgt tgacgaaggc
     2341 gcgcggcccg gactcctcga ggcggaacga ctcggggtgg ccaccgaccg ggatgctcgc
     2401 gacgagcttc aggctcgaag gctcgatcac gccgagcgcg ccctcgccgt agccgagata
     2461 gagctggccg gcggccgcgt cgtaccgcag gttgtctgcg tctcccaggt ccggcagcgt
     2521 ggccgtccgg cgataggtca cgtcgtcgaa cgccgcgacc gctccgccgc gcgtcgcgac
     2581 cacgatccga tggaggaccg gcaggtaggc gaccccctgc ggctccttca gccctgcgat
     2641 gctcgtgatg cgggttccgg cgccgatgtc cagcacctcg agggtgccat ggcccagcgc
     2701 cgcgacgaac aaccgcttcc ctgctgggtc catcgcgaag tgatcgatgc gacgttcgac
     2761 gcccggcagc gggatcacct gcaccgcgga gagcggcggc ggcgcggcgc gggcccgcgg
     2821 agcgaccgac gcgaagccga cgagcccgag cgtgatcaac cgccgtcgca gcacgcgcat
     2881 gccgttcttc tacggccagg acgcgtcgct cgcaaccggg accggcggta gactccaggg
     2941 ccgctcgccg gcaggccatc ggtcatgacg agacagcccg tcctcgccct cacgctcgtg
     3001 ctcctcgcga ccgccgtcgc gcggacggct cccgcgccgg cggcgccccc gacggcgcca
     3061 tcgcttccgc tgtccttggt tgcggacctc cccttgcccg ggaagccctc gcgcttcgac
     3121 taccagtggg tcgatgcggc gcagcggcgc ctgttcatcg cccacctcgg cgacagctcg
     3181 ctcctggtgt tcgatctgga cagccagcgc gtgatccagg aagtgccgca cctcccgagc
     3241 atccacggcg tcgtcgccgc cccggcccag cacctcgtgc tcgccacggc gacggcggac
     3301 acgacccgcg cgctcatcga cgaccggacc ttccaggtga cggcgcgcgt accggcaggc
     3361 gagtacccga acgggctcgc cttcgacccc gcgtcgggca aggtcttcgt ctcgaacaac
     3421 accggccggg gcgtggcggt ggtcgacgtg gccgccgccc gggcgctgcc gggcatcgac
     3481 atcggaggag gcgccggcaa cacgcagtgg gacgccgcgt cgggccatgt ccttgcggcc
     3541 gtccatggct cgccgtctct ggtcgatatc gatccggcca cgtcccggat cgccgggcgc
     3601 atcgcgctcc acggcgtgac cacgtgccat gggctgctcg tggcatcgac gccggggctc
     3661 gcgttcgccg cgtgccgagg cgcggcgccg gtcctggcgg tcgtcgacgt gcgcgcgcgg
     3721 cgccagacga tgaccctgcc gctgccggcc ggcatcgacg tgctcgcctt cgatcccggc
     3781 ctgcagaggc tctatgcagc gtccgagacc ggcacggtcg cggtgttcgc cgtcgccgcc
     3841 gatcgctccg tcaccgagat gggccacggc ttcctcggcc cgaacgcgca cagcgtggcg
     3901 gtcgatccgg ccacgcaccg ggtgtacttc ccgctggaga acgtgggcgg gcaccccgtc
     3961 ctgcgcgtca tggacgcgcg cgtcgcgacg ggctctcctt gatctccacc ctgcggagcg
     4021 cggtcgcctc gctcgaggcg cgatcccacg agggctacgt cagcgcgtcg agcgcggacg
     4081 ccaggaccgc cgcgcgcgcg ggatcgatca tcgcgatata ggcgatcttg ccccggaggt
     4141 aggcggcgaa gtcgggatga tggtcgcggt tctgggcggc caggccggtg gtccgggcgc
     4201 gatgcaggat ggcgcgcagg cggcggagct cctcgcgcgg gacgccgggc ctgtcgttga
     4261 cgacgacgcc ggtgacggac tggcgctgcg accggcgctg gacccgacct ttgcgctcgt
     4321 tcacggcgaa gccctcgtcg cgcacgatgt ggcggacgcg ggccaggagc agcgcgaggc
     4381 tgccggcggc cgccgtgccg gtggagaacg tcagatcgtc ggcgtagcgc gtgtaggtcc
     4441 agccgagctt gccggcagca ccggcgagcc ggcggtcgag cttgcgggtc accaggttcg
     4501 agatcgccgg gctggtgcac gcgccctggg gcagcgcgcg atcgccggcg gcgacccaga
     4561 acgtgacgcc gtcgtggacg accgccgacc gcggagactc ggtgcacagg agcgcgagca
     4621 gggtcgccac ggcgggcgag taaccgatcg attcgaacag gccgcggacc cgcggaaacg
     4681 tgatcgtcgg aaagaactcg gcgagatcga gattgaccac gatgtccttg ccgacgtgca
     4741 ggcgcgcgtt gctgatcgtc gagcgtccgg tcacgaagcc gtgcgcgtgc ggggtcacct
     4801 cgagccgggc gagcacgtgg gcgagcaccc agcgctgggc cgcggcgagc cgggcgtgcg
     4861 gtgccgccag cagccgggtg ccgcccgagc gcttggggac ctcgaagtag acgtagtggg
     4921 tcttctgcgg ggcctcggcg tggaagcaca gccagcgcag ctgcgggatc gagagcccga
     4981 gcgcctccgc gaggtcggcc ggcgtggcca gcacgggcac gccgagctgt gcgagctgct
     5041 cgacgtgggc gcgccggtcg tgccgccggg ccgagacgcc gcgcccggcg aacacgatct
     5101 cggtcgcgcg gcggcgggcg ac
//
LOCUS       DASVOR010002272         4645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_492417,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002272 DASVOR010000000
VERSION     DASVOR010002272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4645
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492417"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VF516_42020"
     CDS             complement(<1..732)
                     /locus_tag="VF516_42020"
                     /inference="COORDINATES: protein motif:HMM:NF044670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114402.1"
                     /translation="MFRRIAGSPGLERLFGRLIRIGRAPEMAPSEARYVVLSNIIAML
                     GVAFTLGFAPILVFSGSPVFPALQVAYALGYLPVLWLNHRHHHTAAATYLVLSSHLLA
                     ISQVLVEGTELDVHLFFMLHTMLPFLVFAPRHDKLMFTLSALAGIDLVLVVALGHHLP
                     HLGPVIAPERLRIIRPILLGGLFATLAACAHYARRATLIAETALDRAHQRSEELLLNI
                     LPPSIARRLKLSGGTIADGFAEVSVL"
     gene            complement(832..1296)
                     /locus_tag="VF516_42025"
     CDS             complement(832..1296)
                     /locus_tag="VF516_42025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114403.1"
                     /translation="MATPATAEPASSGMTATAEPGNTSDPSNSYNPSNSDSSSATSPG
                     ATVPGHPQLPSINDPDRTGSQRTPSGSGAAPTGNGTDTGSDMSNPPDTTTPTLGKDVG
                     SGANTGTGTGSNTRRRTGTTSGSSAKTRSGSNSGSNGSGSADGSGGGSGSGQ"
     gene            complement(1531..2154)
                     /locus_tag="VF516_42030"
     CDS             complement(1531..2154)
                     /locus_tag="VF516_42030"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEX8114404.1"
                     /translation="MCKAIVFSIAILAGGTLAACGSDHDHDDVEASAIEQGTTRGQAL
                     QAQAAQELAGCTESEAIARAAGVVFAIHNGEIAQAQFVLSKTANPGVRNLATQIVADH
                     QASNVALMTLLHNRGLAPVETSVSRTLTSEASAGLAQLQAGELRNLHIDYTQMQISMH
                     QEASLIVGTVRDSMPASAAELRGFLTDTLTVIDKHLTHAEAVLRDLP"
     gene            complement(2237..3190)
                     /locus_tag="VF516_42035"
     CDS             complement(2237..3190)
                     /locus_tag="VF516_42035"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEX8114405.1"
                     /translation="MSLPPVLRGRLRIPVVGAPMFIISQPELVVAQCTAGIVGSFPAL
                     NARPPDLLDTWLTRIRDELAAWDAAHPDQPAAPFAVNQIVHKSNDRLAHDLDLCVKHQ
                     VPIVITSLGAREDVNQAVHSYGGIVLHDVINVVHARKAIDKGADGLIAVCAGAGGHAG
                     MLSPFALIDEIRGWWDGPLLLSGAIANGRSVLAAQALGADLAYVGSAFIATTEAHASD
                     RYKAAIVEATAADIIYTNLFTGVLGNYLRSSIVNAGLDPDNLPTSDPSKMNFGSASTK
                     AWRDIWGCGQGIGGIHEVTPVAAMVDRFATEYAAARQRLCS"
     gene            complement(3279..3542)
                     /locus_tag="VF516_42040"
     CDS             complement(3279..3542)
                     /locus_tag="VF516_42040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114406.1"
                     /translation="MRTTTKLLGFGSAIGAAVMLGRRRRMTLQARSADPGDLQLPVGI
                     SEVDPQPLTQISAEGIDPDATRAAHDEPREQRARLPVHGQNLP"
     gene            3635..>4645
                     /locus_tag="VF516_42045"
     CDS             3635..>4645
                     /locus_tag="VF516_42045"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114407.1"
                     /translation="MIPQDELDHRGSATGATAGATRGHRAPRGRVVVVTGASSGLGRA
                     IAIEFARRHSSLVLGARRTEALEDTARSCRIAGGEAIAVATDVTREQDVIELAAAALD
                     RWDRIDVWINNAGVTLFALLEQAPFEQHQRVIETNLLGAMLGARAVVPVFRRQHRGML
                     INIGSVLSEIGQAFVPSYVISKFGVHGLSEALRVELADEPDIHVCTVFPYAIDTPHFE
                     AAGNRLGQVPRAMPPMSSPERIARAVAKLPDHPRRRLYVPRVAVLGIAAHAIIPRTLE
                     RLVLDALRAWNISEQSEQVSTGNLFEPGPETPGTRGNRPPQVGTARLLAWLAARLVRL
                     EAE"
BASE COUNT          642 a         1590 c         1736 g          677 t
ORIGIN      
        1 cagcaccgag acctcggcga agccgtccgc gatcgtgccg ccggacagct tgagccggcg
       61 ggcgatcgac ggcggcagga tgttgagcag cagctcctcg gagcgctggt gcgcgcgatc
      121 gagcgcggtc tcggcgatca gcgtcgcgcg gcgtgcgtag tgggcgcatg cggcgagcgt
      181 ggcgaacagc ccaccgagca ggatcggccg gatgatccgc agccgctcgg gcgcgatcac
      241 cgggccgagg tggggcaggt ggtggccgag cgcgacgacc agcaccaggt cgatcccggc
      301 gagcgccgac agcgtgaaca tcagcttgtc gtggcgcggc gcgaacacca ggaacggcag
      361 catggtgtgg agcatgaaga acaggtggac gtcgagctcg gtgccctcga ccaggacctg
      421 cgagatcgcg agcaggtggc tgctcagcac caggtaggtc gccgccgccg tgtggtggtg
      481 gcggtggttg agccagagca caggcaggta accgagcgcg taggcgacct gcagcgcggg
      541 gaacaccggc gagcccgaga acaccaggat cggcgcgaac ccgagcgtga acgcgacgcc
      601 gagcatcgcg atgatgttgg acagcacgac gtagcgcgcc tccgacggcg ccatctcggg
      661 cgcgcggccg atccggatca gccggccgaa cagccgctcg aggcccggtg atcccgcgat
      721 ccgccgaaac attcggcgca ctgtatcgcg accggtacat cgccgtcacg ccgcgcgcgc
      781 cggccgcggc caccggccac ggccaccggc cacggccgat cgatcaccgg ctcactgtcc
      841 cgagccgctg ccgccgccgc tgccgtcggc cgagccgctg ccgttcgagc cgctgttcga
      901 accgctgcgc gtcttcgcgc tcgaaccgct ggtcgtgccg gtgcggcggc gggtgttgct
      961 gccggtgccg gtgccggtgt tggcgccgct gccgacgtcc ttgcccaggg tcggcgtggt
     1021 cgtatcgggc gggttgctca tgtcgctgcc ggtgtcggtg ccgttgccgg tcggcgctgc
     1081 gcccgacccg gatggagtcc gctgcgagcc ggtgcggtca gggtcgttga tcgatggcag
     1141 ttgcggatgg cccggcaccg tggcacccgg cgacgtggcg ctgctgctgt ccgagttgct
     1201 gggattgtac gagttgcttg gatcgctggt gttgccgggc tcggccgtgg cagtcatgcc
     1261 gctcgacgcg ggctcggccg tggccggagt cgccacggga tggtcgtggt gacacgcagg
     1321 cgcagccgca gcgaggagga cggagacgag gaatgagctc ttgatcatgt cgctttctcc
     1381 agagtctaag aagggaaaat tctctgagca tcgctgtcgg atgcccgcgg acggactcgg
     1441 acaaagccct tgcgatcgcc atgccacgca gcgacggcgg cgctcacccg cgcgtgcgga
     1501 gctcacgagc acgcgcgtgg ccgtcggcgc tcacggaaga tcgcgcagca cggcctcggc
     1561 gtgcgtgagg tgcttgtcga tgaccgtcag cgtgtcggtc aggaagccgc ggagttcggc
     1621 cgccgacgcc ggcatggagt cgcgcacggt gccgacgatc agcgacgctt cctggtgcat
     1681 cgagatctgc atctgggtgt agtcgatgtg gaggttgcgc agctcgccgg cctggagctg
     1741 ggcgaggccg gcgctcgcct cgctggtcag cgtgcgcgac accgaggtct cgaccggcgc
     1801 cagcccccgg ttgtgcagca gggtcatcaa cgccacgttg ctcgcctggt gatcggcgac
     1861 gatctgggtc gccagattgc gcacgccggg gttggcggtc ttcgacagca cgaattgcgc
     1921 ctgggcgatc tcgccgttgt ggatcgcgaa cacgacgccg gccgccctgg cgatcgcctc
     1981 gctctccgtg cagcccgcga gctcctgcgc tgcctgggcc tggagcgcct ggccgcgggt
     2041 ggtgccttgc tcgatcgcgc tggcctcgac gtcgtcgtgg tcatggtcgc tgccgcacgc
     2101 cgcgagcgtt ccgcctgcga ggatcgcgat gctgaagacg atggctttgc acatggccgc
     2161 cgtggctgca cgcggtatgc cccgcgcaac tcgcatgcgt cgagggctga catcgcggca
     2221 gcggcgcgcg gcagcgctag ctgcacagcc gctggcgcgc ggccgcgtac tcggtggcga
     2281 accggtcgac catcgccgcg accggcgtga cctcgtggat cccgccgatg ccctggccgc
     2341 agccccagat gtcgcgccac gccttggtcg aggcgctgcc gaagttcatc ttcgacggat
     2401 cgctggtcgg caggttgtcg ggatcgaggc cggcgttgac gatcgacgat cgcaggtagt
     2461 tgccgagcac gccggtgaac aggttggtgt agatgatgtc cgccgcggtc gcctcgacga
     2521 tcgccgcctt gtagcggtcg gacgcgtggg cctcggtggt cgcgatgaac gccgagccga
     2581 cgtaggccag gtcggcgccg agcgcctggg ccgcgagcac cgagcggccg ttggcgatcg
     2641 cccccgacag cagcagcggg ccgtcccacc acccgcggat ctcgtcgatc agcgcgaacg
     2701 gcgacagcat gccggcgtgg ccgccggcgc cggcgcacac cgcgatcagt ccgtcggcgc
     2761 ccttgtcgat cgccttcctg gcgtggacga cgttgatcac gtcgtgcagc acgatgccgc
     2821 cgtagctgtg caccgcctgg ttgacgtcct cgcgcgcgcc gagcgaggtg atcacgatcg
     2881 ggacctggtg cttgacgcac agatcgaggt cgtgggcgag ccggtcgttg gacttgtgca
     2941 cgatctggtt gaccgcgaac ggcgcggccg gctggtcggg atgggcggcg tcccacgcgg
     3001 cgagctcgtc gcggatccgg gtcagccagg tgtcgagcag gtcggggggc cgcgcgttga
     3061 gcgccgggaa cgatcccacg atccccgccg tgcactgggc gacgaccagc tcgggttgcg
     3121 agatgatgaa catcggcgcg ccgacgaccg ggatccgcag ccgcccgcgc aggaccggag
     3181 gaagactcat gcgcccacgt taacaacttc gcgggcgcag gtgcgagtgt tctcgaccgc
     3241 cgcgcgccga tccgggctca ccgcccccgg cgccgcggtc acggcaggtt ctggccgtgg
     3301 acgggcaggc gcgcccgctg ctcgcgtggc tcgtcgtgcg ccgcgcgggt cgcgtcgggg
     3361 tcgatcccct cggccgagat ctgagtcagc ggttgcggat cgacctccga gattcccacg
     3421 ggcagctgca ggtcgccggg gtcggcggac cgcgcctgca aggtcatccg gcggcggcgg
     3481 cccagcatca ccgctgcgcc gatcgccgac ccgaaaccca ggagcttggt ggtagtcctc
     3541 atgcggagca gcgacgcaac cgccgtgccg caccggcggc gcccgggccg gtcggccgcg
     3601 gcgtgccggc acgcggtctg cactctcggc ggtcgtgatc ccgcaagacg agctcgacca
     3661 ccgaggatcg gcgacgggcg ccacggccgg cgcgacacga ggacacaggg cgccgcgcgg
     3721 ccgcgtcgtg gtcgtcaccg gggcgtccag cgggctcggc cgggcgatcg cgatcgagtt
     3781 cgcacgccgc cacagctcgc tggtgctggg cgcacgccgg accgaggcgc tggaggacac
     3841 cgcgcggagc tgccggatcg cgggaggcga ggcgatcgcg gtcgcgaccg acgtgacccg
     3901 cgagcaggac gtcatcgagc tcgccgccgc cgcgctcgat cggtgggacc ggatcgacgt
     3961 ctggatcaac aacgccggcg tgacgctgtt cgcgctgctc gagcaggcgc cgttcgagca
     4021 gcaccagcgc gtgatcgaga ccaacctgct cggtgcgatg ctcggagccc gcgccgtggt
     4081 gccggtgttt cgccgacagc accgcggcat gctgatcaac atcggctcgg tgctgagcga
     4141 gatcggccag gcgttcgtgc cgtcgtacgt gatcagcaag ttcggggtgc acgggctgtc
     4201 ggaggcgctg cgggtcgagc tcgccgacga gcccgatatc cacgtgtgca cggtgttccc
     4261 gtacgcgatc gacaccccgc acttcgaggc cgccgggaac cggctcggcc aggtgccgcg
     4321 cgcgatgccg ccgatgagct cgcccgagcg gatcgcccgg gcggtcgcga agctgcccga
     4381 tcatccgcgg cgccggctct acgtgccgcg ggtcgccgtg ctcgggatcg ccgcccacgc
     4441 gatcatcccg cgcacgctcg agcgcctggt gctcgacgcg ctgcgcgcct ggaacatcag
     4501 cgagcagtcg gagcaggtct cgaccggcaa cctgttcgag cccggccccg agaccccggg
     4561 gacccgcggc aaccggccgc cgcaggtcgg gaccgcgcgg ctgctggcgt ggctcgccgc
     4621 ccggctggtc cgcctcgagg ccgag
//
LOCUS       DASVOR010002273         3390 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113255,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002273 DASVOR010000000
VERSION     DASVOR010002273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113255"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VF516_42050"
     CDS             complement(<1..326)
                     /locus_tag="VF516_42050"
                     /inference="COORDINATES: protein motif:HMM:NF025132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT domain-containing protein"
                     /protein_id="HEX8114408.1"
                     /translation="MVRRGLVLTLALALASGPADAFELPRPMGMYPVRSGPALAMLDS
                     RIEVTVRGPIVEAVVVQRFRNTGDRATEASYIFPLPADAAVSAMAMRIGSRTIHAAIE
                     PREAAQR"
     gene            complement(440..1771)
                     /locus_tag="VF516_42055"
     CDS             complement(440..1771)
                     /locus_tag="VF516_42055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HEX8114409.1"
                     /translation="MTWRRPDNLRRIVERLVQDDFIGEVLVWRNDPAVALDLPWPKVR
                     VIDSAENVICHGRYRCAEQAAYPLVYTQDDDVLVHDVAALVRQFQADPTRIHFNLSDW
                     HHARRDRHLYGDSHCALVGWGAVFAKDWLSVLERVPEPVRSSALFRREADTYFTMLQR
                     RHHAPHRGALTNLDGHSTAGLALWCAPEHPRMSSLAVRDALGVIRGSRGFSLPPLWHV
                     VVTCHNYARYLGEAVESVLLNDADYELTIVDDASPDDTPEVARELRARYPHVRYLRLP
                     ERAGVSRARNVGIATLDSAYVALLDADDRFGGDYLFEAGRVLGGGADIANPDALLFGL
                     ESGRWSTPATTTLAELLRHNSVHYCAAFRRSWWAEIGGFDEDIEDWEDYDFWIRAVAR
                     GARVRAVGGDHFHYRRHASSRSSENAGVRDRLRRMMRGKHEPLFAQCRVPE"
     gene            complement(1819..>3390)
                     /locus_tag="VF516_42060"
     CDS             complement(1819..>3390)
                     /locus_tag="VF516_42060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8114410.1"
                     /translation="HCKGEDCAEFAPPGLIRIREPLATAAPSVVPAAALAIIAPAPEP
                     ASVAEGPPAITAGDRPLVSCIMPTRDRRAWLPQAIGYFLRQDFTDAELVIVDDGEPAV
                     RDLVPEHPRIRYERIDGRRTIGAKRNLACVAARGELIAHWDDDDWYPASRLRRQVEAM
                     RAARAELCGTSRLYFYQPSADQAWCYEFAARGTPGLVGTSLMYRRQLWERHRFADIQV
                     GEDTRFVRAGAAAVHDLMDPALCVATIHPANTSPRNPGAVYWRPQPAEDIHRLLGDDL
                     AFYRRVAAPMISCIMPTADRRAFVPLGVRGFLAQDWPNRELIVVDDGRDAVGDLVAGV
                     PGVRYLRLPGRASIGAKRNLACGEARGELIAHWDDDDWYAPGRLSWQAAPILAGAADL
                     TGLENRFILQLPVGEFWTTRRELRQRMFAGDVIGGTMMFRRSLFAGGLRYPEANIAED
                     AAFLREAIRRGHRLAQLDNPGLFVYVRHGRNTWQFEPGRFLDPHGWEVVAGPSSLPGE
                     TLDAYRAAATGMTTP"
BASE COUNT          515 a         1298 c         1136 g          441 t
ORIGIN      
        1 cgctgcgccg cctcgcgggg ctcgatcgcc gcgtggatcg tgcggctgcc gatccgcatc
       61 gccatcgccg acaccgcggc gtcggccggc agcgggaaga tgtagctcgc ctcggtggcg
      121 cggtcgccgg tgttgcggaa ccgctggacg accaccgcct cgacgatcgg gccgcgcacc
      181 gtgacctcga tccggctgtc gagcatcgcg agcgccgggc cgctgcgcac cgggtacatc
      241 cccatcggcc gcggcagctc gaacgcgtcc gccggccccg acgcgagggc cagggccagc
      301 gtcaggacca ggccgcggcg caccatgggg cgagcttaca cacgcacggc gggtgccgat
      361 cggttcaaac cggtcgatgc cggcgggtga cactcgataa catcttggaa tcgctgaccg
      421 ccgtcacacg accctcgagt cactcgggca cgcggcactg cgcgaacagc ggctcgtgct
      481 tgccacgcat catgcgccgc agtcgatcgc gcacgccggc gttctcggag gaccgcgagg
      541 acgcgtggcg ccggtagtgg aagtgatcgc cgcccaccgc gcgcacccgc gccccccggg
      601 caaccgcacg gatccagaaa tcgtagtcct cccagtcctc gatgtcctcg tcgaagccgc
      661 cgatctcggc ccaccagctg cgccggaacg ccgcgcagta atgaaccgag ttgtggcgca
      721 gcagctccgc gagcgtcgtc gtggccggcg tcgaccaccg gcccgattcc aggccgaaca
      781 gcagcgcatc cgggttggcg atgtcggcgc cgccacccag cacgcggccg gcctcgaaca
      841 ggtagtcgcc tccgaaccga tcatcggcat cgagcaacgc cacgtacgcg ctgtcgagcg
      901 tggcgatccc gacgttgcgc gcgcgactga ccccggcgcg ctcgggcaga cggaggtagc
      961 gaacatgcgg gtatcgcgct cgcagctcac gggcgacctc cggcgtgtcg tccggcgacg
     1021 catcgtcgac gatcgtcagc tcgtaatcgg cgtcgttgag cagcacggac tccaccgcct
     1081 cgccgagata gcgcgcgtag ttgtgacagg tcacgacgac gtgccacagc ggagggagcg
     1141 agaatccccg cgatccgcgg atcacgccca gcgcatcgcg caccgccagc gacgacatcc
     1201 gagggtgctc cggcgcgcac cacagcgcca gccccgcggt cgagtgcccg tcgagattcg
     1261 tcagcgcgcc gcgatgcgga gcatggtgcc ggcgctgcag catcgtgaag tacgtgtccg
     1321 cctcgcgccg gaacagcgcg ctcgaccgca ccggctcggg cacccgctcg agcaccgaca
     1381 gccagtcctt cgcgaacacc gcgccccagc cgaccagcgc acagtgactg tctccataga
     1441 ggtgtcgatc gcgccgcgca tggtgccagt cgctcaggtt gaagtggatc cgcgtcggat
     1501 cggcctggaa ctgccgcacc agcgcggcca catcgtgcac cagcacgtca tcgtcctgcg
     1561 tgtacaccag cggatacgcc gcctgctccg cgcaccggta gcggccatgg cagatcacgt
     1621 tctccgccga gtcgatcacc cgcaccttgg gccatggcag gtccagcgcc accgccggat
     1681 cgttgcgcca caccagcacc tcgccgatga agtcgtcctg gaccagccgc tccacgatcc
     1741 gccgcaggtt gtcgggtcga cgccaggtca cgaggcacgc agagatcgtc ggcagcatcg
     1801 gtcacctccc ttccatgctc atggtgtggt catcccggtc gccgcagcgc gatacgcatc
     1861 gagcgtctcg cccggcaacg acgagggccc ggccaccacc tcccagccgt gcggatcgag
     1921 gaatcgacct ggctcgaact gccaggtgtt gcggccgtgg cgcacgtaga cgaacagccc
     1981 cgggttgtcg agctgcgcca gccggtggcc gcgccggatc gcctcgcgga ggaacgccgc
     2041 gtcctcggcg atgttggcct ccggatagcg cagcccaccg gcgaacagcg agcgacgaaa
     2101 catcatcgtc ccgccgatca cgtcgccggc gaacatccgc tggcgcagct cgcgccgcgt
     2161 ggtccagaac tcccccaccg gcagctgcag gatgaagcgg ttctccaggc ccgtcaggtc
     2221 cgccgcgccg gcgaggatcg gcgccgcctg ccagctcagc cggcccggtg cgtaccagtc
     2281 atcgtcatcc cagtgcgcga tcagctcgcc gcgcgcctcg ccgcacgcca ggttgcgctt
     2341 ggcgccgatc gaggcgcgcc cgggcagccg caggtagcgc acgcccggca ccccggcgac
     2401 cagatcgccc accgcgtcgc gcccgtcatc gacgacgatc agctcgcggt tgggccagtc
     2461 ctgcgccagg aagccgcgca ccccgagcgg cacgaacgcc cgccggtccg ccgtcggcat
     2521 gatgcacgag atcatcggcg ccgccacccg ccgatagaac gccaggtcgt cgccgagcag
     2581 gcgatggatg tcctcggccg gctgcggccg ccagtacacc gcgcccgggt tgcgcgggct
     2641 ggtgttcgcc ggatggatcg tcgccacgca cagcgccggg tccatcaggt cgtgcaccgc
     2701 ggccgcaccg gcgcgcacga accgcgtgtc ctcgccgacc tggatatcgg cgaaccggtg
     2761 gcgctcccac agctgccgcc ggtacatcag cgacgtgccg accagcccgg gcgtgccccg
     2821 cgccgcgaac tcgtagcacc aggcctggtc cgccgacggc tggtagaagt acagccggct
     2881 cgtcccgcac agctccgcgc gcgccgcgcg catcgcctcg acctgccggc gcagccgcga
     2941 cgccgggtac cagtcgtcgt catcccagtg cgcgatcagc tcgccgcgcg ccgccacgca
     3001 cgccaggttg cgcttggccc cgatcgtgcg gcggccgtcg atccgctcgt agcggatccg
     3061 cgggtgctcg gggaccaggt cgcgcaccgc cggctcgccg tcgtcgacga tcaccagctc
     3121 ggcgtcggtg aagtcctggc gcaggaagta gccgatcgcc tgcggcagcc acgcccgccg
     3181 gtcgcgcgtc ggcatgatgc acgacaccag cggccgatcg cccgccgtga tcgccggtgg
     3241 cccctcggcc acggacgccg gctcgggcgc gggcgcgatg atcgccagcg ccgcagcggg
     3301 aacgacgctc ggcgcggcgg tggccagcgg ctcgcggatc cggatcagcc ccggcggcgc
     3361 gaactccgcg cagtcctcgc ccttgcagtg
//
LOCUS       DASVOR010002274         2828 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_345450,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002274 DASVOR010000000
VERSION     DASVOR010002274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345450"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF516_42065"
     CDS             complement(<1..304)
                     /locus_tag="VF516_42065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114411.1"
                     /translation="MSLARPTALVIHDDGEALDVLTRLFEAAGFEVTTAITGFRAQAH
                     LEGERPIDVVVVPWDAAHPVGGEVYRWSLQRRYDLRDGFVFLAAETPPDFDRLVAGR"
     gene            372..2210
                     /locus_tag="VF516_42070"
     CDS             372..2210
                     /locus_tag="VF516_42070"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEX8114412.1"
                     /translation="MLSLGALGVLTWGLAACAHGPSSTAGGAGPTPPLPPGGAAGPEL
                     GDSKLELPAIPARIEVTTSAPTDGTAPDKHSPILDVLKAENEREMAALGKQKDPAYYL
                     GYQLVEQRVVNLESEGGALITDSDDTLRNLDVEVRVGKPELDNTRALADDSNGLNAPL
                     TRRGTVPFGDDKQALSNALWLETDRRYREAVSALGYVRQDQSTLKNRVVAPDFSPEPP
                     EVHIEASTKLTFDKAQWIERLKRCSAKALHGQATRGSCGVLFQQNTAYLVNSEGSRIQ
                     TSWTNAQLSVSAGVKADDGMNLSRLEQRFGRTPADLPPDAEVDKMIATVTGDIDALHE
                     APLADPYVGPAILEGRAAGVFFHEVFGHRIEGHRQKDLTSGQTFASYVGKDIAPDWLS
                     VYDDPEIVTLNGVQLNGFYRYDDEAVRARRVSLVDHGKLVGFLMGRNPIPGFGHSNGH
                     GRRQPSLPPVARQGNLVVEVTKSVKRDELEKMLIEEIKRQHRPYGMIFTDISGGFTNT
                     SAFAPQAFKVNPVMAYRLYPDGRKELVRGIDISGTPLVALQSIRAASREIETFNGVCG
                     AESGWVPVSASAPSLLLEKLEVEKGFIPPDRPPVLQPPAIRQSERP"
     gene            2216..>2828
                     /locus_tag="VF516_42075"
     CDS             2216..>2828
                     /locus_tag="VF516_42075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114413.1"
                     /translation="MRNTRVARRWTTSLAALGVFAGGALLAPGTAPAGPAPDPASKEP
                     PRPAPRPPDRDVSKTGKDAPKASPSLGPPSKAVTNEILDAIAEEMNRAMTQLEIPGAP
                     KPYHIAYKITEVDVNDCAASLGYTTSRRNRHFVNLEARVRVGSPQLDNGNFVVPGADE
                     LDAVAGINLSLEATPRIARRAAWLVTDSAYKEALIQLRAKLEAR"
BASE COUNT          472 a         1044 c          971 g          341 t
ORIGIN      
        1 agcgccccgc gaccagccgg tcgaagtccg gcggggtctc ggcggcgagg aacacgaagc
       61 cgtcgcgcag gtcgtagcgg cgctgcagcg accagcgata gacctcaccg ccgaccgggt
      121 gcgccgcgtc ccacggcacc acgacgacgt cgatcgggcg ctcgccctcg aggtgggcct
      181 gcgcacggaa tccggtgatc gcggtggtga cctcgaaccc ggcggcctcg aacagccgcg
      241 tcagcacgtc gagcgcctcg ccatcgtcgt ggatgaccag cgcggtggga cgggccagcg
      301 acaaggtggg tacatgatag ctgcaggtgg cgtacaggat agccgtgcta gtgtttggac
      361 atatgcgatg gatgctttcg ctcggcgccc tgggcgtgtt gacctggggg ctcgcggctt
      421 gcgcgcacgg ccccagcagc acggccggcg gcgcaggccc cacgccaccg cttcccccgg
      481 gtggggcggc cggccccgag ctcggcgaca gcaagctcga gctgcccgcg atccccgccc
      541 ggatcgaggt cacgaccagc gcgccgaccg acggcaccgc tcccgacaag cactcgccga
      601 tcctcgacgt cctcaaggcc gagaacgagc gcgagatggc ggcgctcggc aagcagaagg
      661 accccgcgta ctacctcggc taccagctgg tcgagcagcg cgtggtcaac ctcgagtccg
      721 agggcggcgc gctgatcacc gacagcgacg acacgctgcg caacctcgac gtcgaggtcc
      781 gggtcggcaa gcccgagctc gacaacaccc gcgcgctggc cgacgacagc aacgggctca
      841 acgcgccgct gacccggcgc ggcacggtgc cgttcggcga cgacaagcag gcgctgtcga
      901 acgcgctgtg gctcgagacc gaccggcgct accgcgaggc ggtcagcgcg ctgggctacg
      961 tccgccagga ccagtcgacg ctcaagaacc gcgtcgtcgc tcccgacttc tcgcccgagc
     1021 cgcccgaggt ccacatcgag gcctcgacca agctgacgtt cgacaaggcg cagtggatcg
     1081 agcggctcaa gcggtgctcg gccaaggcgc tgcacggcca ggcgacccgc ggcagctgcg
     1141 gcgtgctgtt ccagcagaac accgcgtacc tggtcaacag cgagggcagc aggatccaga
     1201 cctcgtggac caacgctcag ctgtcggtgt ccgccggggt caaggccgac gacggcatga
     1261 acctgtcgcg gctcgagcag cggttcggcc gcacgccggc cgacctgccg cccgacgccg
     1321 aggtcgacaa gatgatcgcc acggtcaccg gggacatcga cgcgctgcac gaggcgccgc
     1381 tcgccgaccc ctacgtcggc ccggcgatcc tggagggccg cgccgccggg gtgttcttcc
     1441 acgaggtgtt cggccaccgc atcgagggcc accgtcagaa ggacctgacg tcgggccaga
     1501 ccttcgcgtc gtacgtcggc aaggacatcg cgcccgactg gctgtcggtc tacgacgacc
     1561 cggagatcgt cacgctcaac ggcgtgcagc tcaacgggtt ctaccgctac gacgacgagg
     1621 cggtgcgggc gcgccgggtc agcctggtcg accacggcaa gctggtcggc ttcctgatgg
     1681 ggcgcaaccc gatccccggg ttcgggcact ccaacggcca cggccggcgc cagcccagcc
     1741 tgccgccggt ggcgcgccag ggcaacctcg tcgtcgaggt caccaagagc gtcaagcgcg
     1801 acgagctgga gaagatgctg atcgaggaga tcaagcgcca gcaccggccg tacggcatga
     1861 tcttcaccga catcagcggc gggttcacca acacctcggc gttcgcgccc caggcgttca
     1921 aggtcaaccc ggtgatggcg taccggctgt accccgacgg ccggaaggag ctggtccgcg
     1981 gcatcgacat cagcggcacg ccgctggtcg cgctgcagtc gatccgcgcc gcgagccgcg
     2041 agatcgagac cttcaacggc gtgtgcggcg cggagagcgg ctgggtgccg gtgtcggcct
     2101 cggcgccgtc gctgctgctc gagaaactcg aggtcgagaa gggcttcatc ccgccggacc
     2161 gccccccggt gctgcaaccg ccggcgatcc ggcagtcgga gcggccatga cccgcatgcg
     2221 gaacacgcgc gtcgcgcgca gatggacgac ctcgcttgcc gcactcggcg tgttcgccgg
     2281 cggcgccctg ctcgcgcccg gcaccgcccc cgccggcccg gcgcccgatc cagcgagcaa
     2341 ggagccgccc aggccggcgc ccaggccgcc cgaccgcgac gtgtccaaga ccggcaagga
     2401 cgcgcccaag gcgtcgccca gcctcggccc gccctccaag gcggtgacca acgagatcct
     2461 cgacgcgatc gccgaggaga tgaaccgggc gatgacccag ctcgagatcc cgggcgcgcc
     2521 caagccgtac cacatcgcct acaagatcac cgaggtcgac gtcaacgact gcgcggcgag
     2581 cctgggctac accacgagcc ggcgcaaccg ccacttcgtc aacctcgagg cccgggtccg
     2641 ggtcggatcg ccccagctcg acaacggcaa cttcgtcgtg cccggcgccg acgagctcga
     2701 cgcggtcgcc ggcatcaacc tgtcgctcga ggccacgccg cggatcgccc ggcgcgccgc
     2761 ctggctggtc accgacagcg cctacaagga ggcgctgatc cagctgcgcg ccaagctcga
     2821 ggcgcgca
//
LOCUS       DASVOR010002275         2945 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_54482,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002275 DASVOR010000000
VERSION     DASVOR010002275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2945
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54482"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            311..1516
                     /locus_tag="VF516_42080"
     CDS             311..1516
                     /locus_tag="VF516_42080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CxxxxCH/CxxCH domain-containing protein"
                     /protein_id="HEX8114414.1"
                     /translation="MRNGWLLILGLVGCAGQVEEGAGSDVAPPNADPQAGCATACHGA
                     DTSNAPPKSVAGVTATTTRAVGAHQAHLVASPAWHRAVECGDCHVVPATVDAPGHIDG
                     DGVAEVIFSTRAGGTASSWNGTSCTAYCHGKTVWGGNAPAPVWTRVDGAQSTCGSCHG
                     VPPPPPHPAGANCAGCHPTMEENSLNFRDPGSHINGVIDMVDQGATGGCTSCHGSASN
                     AAPPRDLRGNTATTVATVGAHQAHLKTSTWHHAVVCTSCHVVPQTVDAPGHIDGDNIA
                     EVKFDTLNPAGVYTKATATCSTMYCHGNGRGGNGTIVFTQAGPLGCTACHSTSGTNMS
                     GHHRTHLGGEVNLKCNACHSQVVDQNMNIINADLHVNGVHEVKMANGTWNATARSCSN
                     TGCHGTKTW"
     gene            1565..1873
                     /locus_tag="VF516_42085"
     CDS             1565..1873
                     /locus_tag="VF516_42085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HEX8114415.1"
                     /translation="MANVLTVVARVRAAKGKGDALAALLVEQAAAVRANEPGCLVYRA
                     HRSTRDPELFLFYEMYADEAALEAHRTAPHLAAFRKRRDQEGLTEGAVEVDVLRSLTD
                     "
     gene            complement(1879..2682)
                     /locus_tag="VF516_42090"
     CDS             complement(1879..2682)
                     /locus_tag="VF516_42090"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8114416.1"
                     /translation="MSAAQTKLVLEQLEDGLLTVVLNRPDRRNALNSELCGQLVETLQ
                     RAAHASEVRAVLLTGAGGTFCVGGDVKAMAEAQAPDRSMHDGVHGLRSRMEASRLLHE
                     MPKPTIAAIAGAAAGAGLALALACDLRVAGESAKITTAFAKVGLSGDFGGTYFLTQLV
                     GSARARELYLTSPVLTGRQALELGLVTRVVADDQVDAAGRELARELASGPTVTFGHIK
                     ANFNLAEHAGLAECLDNEAWRHITCLTTADHREAATAFVEKRPPRFRGK"
     gene            2758..>2945
                     /locus_tag="VF516_42095"
     CDS             2758..>2945
                     /locus_tag="VF516_42095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114417.1"
                     /translation="MRTFALSASAALALALAACARWLPAPVPMRSIAWPHISSQQAAS
                     RARCLVVFLPGLGDDADD"
BASE COUNT          420 a         1079 c         1075 g          371 t
ORIGIN      
        1 gggcggcggc ggatcgggga ccgccgcggc gggggccggg gcggcgacgg gcggcactgc
       61 gcggggaggc cgggcgcgct cggtggacga gcaggcggcg agcacgacga ggaggccagc
      121 acgcatgcgc cgacgatacg cgacgtctgc gctgcgaccc gccggacgag cggccgtcgc
      181 ggcagtgcgc gcggcgccta catccccgac gacgttgcgc tgacagtcgt ccggaatttg
      241 cacatgcgcg aaaccagtgg gtgttagtcc agccacagcg gcgcgcgggg cgatctgggt
      301 acgctgaggc atgcgaaacg gctggctcct gatcctcggc ctcgtgggct gcgcggggca
      361 ggtcgaggag ggcgccggca gcgacgtggc cccaccgaac gccgatccgc aggccgggtg
      421 cgcgaccgcc tgtcacggcg cggacaccag caacgcaccg ccgaagtccg tcgcgggcgt
      481 caccgccacc acgacgcgcg cggtcggcgc gcaccaggcg cacctggtcg cgtcgccggc
      541 atggcaccgc gcggtcgagt gcggcgattg ccacgtcgtc ccggcgaccg tcgacgcgcc
      601 cggccacatc gacggggacg gcgtcgccga ggtgatcttc agcacgcgcg ccggcggcac
      661 cgcgtcgtcg tggaacggca cgagctgcac ggcctactgc catggcaaga ccgtgtgggg
      721 cggcaacgcg ccggcgccgg tgtggacgcg ggtcgacggc gcgcaatcga cgtgcggcag
      781 ctgtcatggc gtgccgccgc cgccgccgca cccggccggg gccaactgcg cggggtgcca
      841 cccgacgatg gaggagaact cgctcaactt ccgcgatccg ggcagccaca tcaacggcgt
      901 gatcgacatg gtcgatcagg gcgcgacggg cggctgcacg tcgtgccacg gctcggcgag
      961 caacgcggcg ccgcccaggg acctgcgcgg caacaccgcg accacggtcg cgacggtcgg
     1021 cgcgcaccag gctcacctca agacctcgac ctggcaccac gcggtggtgt gcaccagctg
     1081 ccacgtcgtg ccgcagaccg tcgatgcgcc gggacacatc gacggcgaca acatcgccga
     1141 ggtcaagttc gacaccctga acccggccgg cgtctacacc aaggcgaccg cgacgtgctc
     1201 gacgatgtac tgccacggca acggccgcgg cggcaacggc acgatcgtct tcacccaggc
     1261 cgggccgctg gggtgcaccg cgtgccacag caccagcggc accaacatgt cgggccacca
     1321 ccggacgcac ctcggcggcg aggtcaacct gaagtgcaac gcctgccact cccaggtggt
     1381 cgaccagaac atgaacatca tcaacgcgga cctccacgtc aacggcgtgc acgaggtcaa
     1441 gatggccaac gggacgtgga acgcgaccgc gcggtcgtgc tcgaacaccg gctgccacgg
     1501 caccaagacc tggtgacgcg gtcccgcgtt ccaaggcggc gccggcccgt gtagagtcgg
     1561 cgcgatggcg aacgtcctca ccgtcgttgc aagggtccgg gccgccaagg gcaagggcga
     1621 cgcgctcgcc gcgctgctcg tcgagcaggc cgcggcggtg cgcgccaacg agccgggctg
     1681 cctggtctat cgcgcgcacc gctcgacccg ggatcccgag ctgttcctgt tctacgagat
     1741 gtacgccgac gaggccgcgc tcgaggccca ccgcacggcg ccccacctcg ccgcgttccg
     1801 caagcgccgc gaccaggagg ggctgaccga gggcgccgtc gaggtcgacg tgcttcgctc
     1861 gctcaccgac tgacgcggct acttgccccg gaaccggggc gggcgcttct cgacgaacgc
     1921 ggtcgccgcc tcgcggtggt cggcggtggt caggcaggtg atgtggcgcc acgcctcgtt
     1981 gtcgaggcac tcggcgagcc cggcgtgctc ggccaggttg aagttcgcct tgatgtggcc
     2041 gaacgtgacg gtcgggccgc tcgccagctc gcgggcgagc tcgcggccgg ccgcgtcgac
     2101 ctgatcgtcg gcgaccacgc gcgtgaccag gccgagctcc agcgcctgtc gtccggtgag
     2161 gaccggcgag gtcaggtaca gctcccgggc gcgcgcgctg ccgaccagct gggtcaggaa
     2221 gtaggtgccg ccgaagtcgc ccgacagccc gaccttggcg aacgccgtgg tgatcttcgc
     2281 cgactcgccc gcgacgcgca ggtcgcaggc cagcgcgagc gccagccccg ccccggcggc
     2341 cgcgccggcg atcgcggcga tcgtcggctt gggcatctcg tgcagcagcc gcgacgcctc
     2401 catgcgcgag cgcaggccgt ggacgccgtc gtgcatgctg cggtccggcg cctgggcctc
     2461 ggccatcgcc ttgacgtcgc cgccgacgca gaacgtgccg ccggcgccgg tcagcagcac
     2521 ggcgcggacc tcgctggcgt gcgccgcgcg ctgcagcgtc tcgaccagct ggccgcacag
     2581 ctcgctgttg agcgcgttgc gccgatcggg gcggttcagc accaccgtca gcaatccatc
     2641 ttccagctgc tccaggacga gcttcgtctg cgctgcgctc acggcggctc ctttcgacgg
     2701 ccgcgactct atcatgcggg atggcccacc ggccccgggc tgggctacac atgggccgtg
     2761 cgcacgtttg cgttgtcggc ctcggcggct ctggcgctcg ccctcgcggc ctgcgcgcgc
     2821 tggctcccgg cgccggtgcc gatgcggtcg atcgcgtggc cgcacatctc gtcgcagcag
     2881 gcggcgagcc gagcgcgctg cctggtggtg ttcctgcccg gcctgggcga cgacgccgac
     2941 gactt
//
LOCUS       DASVOR010002276         8285 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_83269,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002276 DASVOR010000000
VERSION     DASVOR010002276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8285
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83269"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..450)
                     /locus_tag="VF516_42100"
     CDS             complement(22..450)
                     /locus_tag="VF516_42100"
                     /inference="COORDINATES: protein motif:HMM:NF024822.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEX8114418.1"
                     /translation="MAVAPVPADIARARTLARVLDHYLVDPLLGLVLPGAGDLIGSLL
                     GLYLVAIAVRRRMSPVIIARMLLHLALDAVLGAVPLIGDIADFAYKANERNLALLVGR
                     HDTGKATARDWLAVGGAAAAFVAVIGGVAYAIVALIRAIA"
     gene            complement(582..1088)
                     /locus_tag="VF516_42105"
     CDS             complement(582..1088)
                     /locus_tag="VF516_42105"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8114419.1"
                     /translation="MRFAIREARLADAEGIARSHTASWRTSYRGILPEAVLAQIDVDQ
                     RTASWRRTLQDRSVLTLVAYDVTHQDIVGLCDAGPSRREPSMGEVYRMYLEHHARRHG
                     LGREMFEQVTAWLRARGLGSLIIWVLDNNQHARRFYEAMGGRAGQRVASTVSGFPVVE
                     LAYVWDRL"
     gene            1355..1693
                     /locus_tag="VF516_42110"
     CDS             1355..1693
                     /locus_tag="VF516_42110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114420.1"
                     /translation="MSVFKNSFGDWSRPPSAPTFARARQLIDDTLNAVEDELSGVPFN
                     PETWLSDGRMYPPQDDNLREVPGRPDVKRLRTKNHNIYIANNGAIRIEQVTSKQIVLD
                     KRGEDGSPVF"
     gene            1702..2238
                     /locus_tag="VF516_42115"
     CDS             1702..2238
                     /locus_tag="VF516_42115"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8114421.1"
                     /translation="MLAKDRVEAFVRLLRKRLPNLTCEVDAPSRAQGDWFIDLKQGDR
                     SFVVQFRSDLGFGLSSTPSEGIGEGPDEFFEDEHDVIERLVELLRTGIRTEPQRVRLL
                     QELRERRELSQVVLASKLGIRQPTLSKMERREDVNLSTLRRYVQALGGELHVTARFPD
                     EAVEIGPPAKSGKRQTSK"
     gene            complement(2442..6014)
                     /locus_tag="VF516_42120"
     CDS             complement(2442..6014)
                     /locus_tag="VF516_42120"
                     /inference="COORDINATES: protein motif:HMM:NF019313.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase fold protein"
                     /protein_id="HEX8114422.1"
                     /translation="MLYNEQEDAIDRIVDALAQRGVSLHYWRRDVAIGDSLNALEEAQ
                     FTAAPLILVLLGARGWEPQQQRLADRALTEQRRMLLAVIGDPPAESLQWANGYFQKQR
                     YADLRKTGPRELDYLAEAIRRRRHSGPAALDAIIGGIINGNEEQRSNLLQQITTKSFT
                     DTAALAARLRDEITGRFAPGRESAYGSAVRDPKLVPSIRAWMLSFLIWSEPESEASRA
                     VVLKHVSHETESDRNVRFWALAGLYQVRAAYLGEAVAAAVNDPAPEVRLLARAIASSS
                     EPSFENDIRADLTSDNFEVAWPVLRVLRVVPLTWLAATVALRLVSSSGQSDPAYDAIY
                     ALASPAMARAAAPVLFEHLGVDGTVRRVIEAGLDAAHTTIARFAEVLVPLDATAVDAA
                     LRAAAPEPRNGAIAAVLRTALERRTGARTSRPVFMAGFHSDAVEEDEAQDADELDIQQ
                     DVQTLTAVMLSTEVRPPLAVGLFGDWGTGKSYFMRAMRRAARALPKSLGPDGKPRFCE
                     RVVPVEFNAWHYVDTNLWASLVSRILERLAAVIAPKPTPEEAQSALLAELASARAVVQ
                     EIDKEKEALAKLSASQQEALGQLRRDRLAKELQLGDLRARDLQQFVEESPELKAKLDE
                     LGLPALMKSVADLDAVAAEARSVGGKAGSVLAAMFDATNRGRLYPLLVLALIVIPALA
                     GLVRHFELIDTALASITAAIAQIVTIAAGVIGVARKSIGVVKQKLTKIVEVQKSVQAF
                     LDEKRKTPSKREKDLERELGELQIKEQEAITKLSVVTARVTELENKLRVLQEGRSLAR
                     FVVERASSEDYRKHMGLISTIREDFQTLSARLAHPPDDEHRVDRIVLYIDDLDRCPAD
                     KVMEVLQAVHLLLAYPLFVVVVGVDPRWLLHSLEESYSAFQSDRPGSATQRLWQTTPQ
                     NYLEKIFQIPFNLRPMSEGGYQRLMRTLMSSSTEATVPKERPADVAAAQRSNHPAPQG
                     RPDTAAGAPQETAQQPQEPATIVPSPQPRPAESRFQVNEEALTISEPEMRFVGRLYAL
                     MPSPRAATRFSNVYRILKAPIQRDQLALFEGTSEVPGSFQIPMLLLAIVIGAPALATS
                     LFPAVFAAARAGKDPRGVFDALHERAGDDSARCILEVIDDPQFPQFPQSANLYVEWIP
                     RVARFSFETGRTIERPGPPEGRRATNGADAHPPGALRPARARAG"
     gene            complement(6278..7024)
                     /gene="gloB"
                     /locus_tag="VF516_42125"
     CDS             complement(6278..7024)
                     /gene="gloB"
                     /locus_tag="VF516_42125"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEX8114423.1"
                     /translation="MRIVSVPCLKDNFAYLVIDDDGRCAVVDPGEAAPVEAALAREHA
                     RLAAVWATHHHPDHVGGIGELVARHGGVEVVIGVKDAAKTPHVTRRVDDGDEVALGPL
                     RARCIHNPGHTVGAITYVVDGCAFTGDTLFGAGCGRLFEGDAATMHTSLMRLAALPPD
                     TRVYFGHEYTATNLRFAAAVEPDNAAVARRAGALATPSTPSTIADERATNPFLRAAEP
                     AVIAAAKARGAAGDPVSVFAALRSWKDGFR"
     gene            7080..8111
                     /locus_tag="VF516_42130"
     CDS             7080..8111
                     /locus_tag="VF516_42130"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8114424.1"
                     /translation="MTMTAMVFREHGGPDVLHLETLPVPEPGPGEVRVRVRAVALNHL
                     DIWTRRGGPAFKLTFPHLLGADIAGVVDAIGPGATAAVGTRVVLQPGLSCGRCAACLG
                     GHDNLCRYYSILGEHKPGGYAEYIVVPDANLAPYPERLDFAHAASSILPFLTAWQMVV
                     HKAQVAPGDTVLVHGAGSGIGVAAIQIAKLYGARVIATAGSDDKVARGRALGADVVIN
                     YSTADFAAECRTLTGKRGVDSVIEHVGGEVFAASIKAVRTGGRIVTCGATAGFHPEID
                     LRHIFFRQIEVLGSTMGSKADLLAVLSHVAAGRLEPVVHGVMPLAEAAAAHRLLENRA
                     AFGKVVLEP"
BASE COUNT         1292 a         2780 c         2851 g         1362 t
ORIGIN      
        1 cactgggggt agcggcctgc gctatgcgat cgcgcggatc agcgcgacga tcgcgtaggc
       61 gactccgccg atcaccgcga cgaacgcggc cgccgcgccg ccgacggcga gccagtcgcg
      121 cgcggtcgcc ttgccggtgt cgtggcggcc gaccagcagg gcgaggttgc gctcgttggc
      181 cttgtacgcg aagtcggcga tgtcgccgat cagcgggacc gcgccgagca cggcgtcgag
      241 cgcgaggtgg agcagcatgc gcgcgatgat caccggcgac atccggcgcc gcaccgcgat
      301 cgcgaccagg tacaggccga gcagcgagcc gatcaggtcg ccggccccgg gcaggaccag
      361 gccgagcagc ggatcgacca ggtagtggtc gagcacgcgg gccagcgtgc gggcgcgcgc
      421 gatgtccgcg ggcacaggcg ccaccgccac ggcgacatcg ctcgacggtg ccacgcgcag
      481 atccccgaac cgccgcgctg cgaaacgtcc tgggtcggcc atgaaccgag ctacagcatg
      541 cgccgtgcca gagcgcgcgg ccagcctgct tgcagcgacc gctacagccg gtcccagacg
      601 taggcgagct cgaccacggg aaaccccgat accgtcgacg ccacgcgctg gccggctcgc
      661 ccacccatcg cctcgtagaa ccgccgggcg tgttggttgt tgtcgagcac ccagatgatc
      721 agcgagccga gcccgcgggc gcgcagccac gcggtgacct gctcgaacat ctcgcggccc
      781 aggccgtggc gccgggcgtg atgctcgaga tacatgcggt agacctcgcc catgctgggc
      841 tcgcggcggc tcggaccggc gtcgcacagc ccgacgatgt cctggtgcgt gacgtcgtag
      901 gcgaccaggg tgagcaccga gcgatcctgc agcgtgcggc gccagctcgc cgtgcgctgg
      961 tcgacgtcga tctgcgcgag caccgcctcg ggcaggatgc cgcgatagct ggtgcgccag
     1021 ctcgccgtgt gggagcgggc gatcccctcg gcgtcggcga gcctggcctc tcggatcgcg
     1081 aaccgcaccc ggcgagtgta gccgcgcggc ccgtgcgcgg gcctgcatgt tcgggcaggt
     1141 gaagaccagc gaaagcttcg aggtaaatcg tgcttgcatg ggcatagccg gaaggctata
     1201 ttccatcgcg gaatgtcgtc gcgatctcct ccagcctggt ccaccaattc ctggtggtac
     1261 tccgtgtcat tgtaatggtg cagcggcggg cccccggtct cacagaagga gttgtatgat
     1321 gaataccctc aagaggctac ggtgcgtccc agaagtgagc gtcttcaaga attccttcgg
     1381 agactggagc aggccccctt ccgcgcctac gttcgcacga gctcgacagc ttatcgacga
     1441 tacgctgaac gccgttgaag acgagctttc gggtgtgccc ttcaatcccg agacatggct
     1501 atcggatggc cggatgtatc cgcctcagga cgacaacctc cgtgaggtcc ccgggcggcc
     1561 tgatgtgaag cgactgcgaa ccaagaacca caacatttat atcgcaaaca atggcgctat
     1621 tcgcatcgaa caggtcacct cgaagcagat cgtactcgac aaacgagggg aggatggctc
     1681 tcccgttttt taggaagaac tatgctggct aaagatcggg tagaagcctt tgtccgcttg
     1741 cttcgcaagc gtcttcccaa cctgacctgc gaggtcgatg caccgtcacg ggcccagggt
     1801 gactggttca ttgatctcaa gcaaggggat cgatcgttcg tcgtccaatt tcggtctgat
     1861 ctgggcttcg gcttgtcttc cactcccagc gagggtattg gcgaaggacc ggacgagttc
     1921 ttcgaggatg agcatgatgt catcgaacgc ctggtagaac tgcttcgcac agggatcagg
     1981 accgagccac aacgcgtgcg cttgcttcaa gagctgcgcg agcgacgaga gctctcgcag
     2041 gtcgtgctgg cgagcaagct cggcattcgt cagccaacgc tctcgaagat ggagcgccga
     2101 gaggatgtga acctgagcac cctgcggcgc tatgttcagg cgctcggcgg tgagttgcat
     2161 gtcacggctc ggtttcccga tgaagcagtg gagatcggtc cgccggccaa gagtggcaag
     2221 cgccagacat ccaaatagag cctcccaagg cgtgccgctg cgggatccgg atcaccttcg
     2281 ctcaggtcac agcgcttcag ttcgcgctac cgggtgatac ccctgcagcc gctggccctg
     2341 catggtaggg cccatggcaa cgtcatggtc aaccggcatc cgcatgtcga gcagcgtcct
     2401 cggatcactt cgaggtccga tcaccgcccg acctggtcgc gtcaaccggc acgagctctg
     2461 gctggccgga gcgccccggg tgggtgcgcg tcggcgccgt tcgtcgctcg acgtccctcc
     2521 ggcggtccgg ggcgttcgat cgttcgcccg gtctcgaacg agaaccgtgc cacccgcggt
     2581 atccactcga cgtagaggtt agcgctctgc ggaaactgcg gaaactgcgg atcgtcgatg
     2641 acctcgagga tacaacgcgc gctgtcgtcg ccagcgcgtt cgtggagcgc atcgaagacg
     2701 ccgcgcggat ccttccctgc ccgtgccgcg gcgaacactg caggaaacag cgacgtggcc
     2761 agtgctggcg caccgatcac gatcgccagt agcagcatcg gtatctggaa gctgcccggg
     2821 acctcgctgg tcccctcgaa cagcgcgagc tgatccctct ggatcggcgc cttgaggatc
     2881 cggtagacgt tggagaaacg cgtggccgct cgcggcgacg gcatgagcgc atacaggcgc
     2941 ccgacgaagc gcatctccgg ctcgctgatc gtcagcgcct cttcattcac ctggaaacgc
     3001 gattcagccg gccgaggttg cggggatggc acgatggtgg cgggctcctg cggctgctgg
     3061 gccgtctctt gcggggcccc tgccgccgta tcggggcggc cctgcggagc tggatgattt
     3121 gaccgctgtg ctgcagcaac gtcggccggg cgctctttcg gcacggtcgc ttccgtgctc
     3181 gacgacatga gcgtgcgcat caggcgttga taccccccct cgctcatcgg gcgcaggttg
     3241 aacggaatct gaaagatctt ctcgaggtag ttctgcggcg tcgtctgcca gagccgctgg
     3301 gtggcggagc cggggcgatc gctctggaac gccgagtaag actcttcgag ggaatggagc
     3361 agccagcgcg ggtcgacgcc gacgacgacc acgaacaggg gataggcgag cagcagatgc
     3421 acggcctgca gcacctccat caccttgtcc gccggacagc ggtcgaggtc gtcgatataa
     3481 agcacgatgc ggtcgactcg gtgctcgtca tctggaggat gtgcgagccg ggcgctaagc
     3541 gtctggaagt cctcgcggat ggtagagatc aggcccatgt gcttgcggta gtcctccgag
     3601 ctcgcgcgtt cgacaacaaa ccgcgcgagg ctccgcccct cctgcaggac gcgcagcttg
     3661 ttctcgagtt cggtcacgcg cgcggtcacg acagagagct tggtgatcgc ttcctgttcc
     3721 ttgatctgga gctcgcccag ctcgcgctcg agatccttct ctctcttgct gggtgtcttg
     3781 cgtttctcgt cgagaaacgc ttgcactgac ttctggacct ccacgatctt cgtaagcttt
     3841 tgcttcacga cgccgatcga ctttctcgcc acgccgatca cccccgcggc gatcgtgacg
     3901 atctgtgcga ttgctgcggt gatgcttgcc agcgcggtgt cgatcagctc gaagtgacgg
     3961 acgagcccgg ccagggctgg aatcacgatc agcgcgagta ccagcaacgg atacagccga
     4021 ccgcgattcg tcgcatcgaa catggcggcg agcacggagc cagctttgcc tccgacggag
     4081 cgggcctccg cagccaccgc gtcgaggtct gccacgctct tcatcaacgc agggaggccg
     4141 agctcgtcaa gcttcgcctt gagctcaggg ctctcctcga cgaattgctg cagatcgcgc
     4201 gcccgcaggt cgccgagttg cagctccttc gccagccggt cccggcgcag ctgcccgagc
     4261 gcctcctgct ggctcgcgct gagcttggca agcgcttcct tctccttgtc gatctcctgc
     4321 acgacggcgc gtgcactggc cagctcggcg agcagggcgc tctgcgcttc ctcgggcgtt
     4381 ggtttgggtg cgatcacggc agctaagcgc tcgagaatcc ggctgaccag gctggcccat
     4441 agattggtgt ccacgtagtg ccaggcattg aactcgaccg ggacgacgcg ctcgcaaaac
     4501 cgcggtttgc cgtccggccc caggctcttc ggcagcgccc gcgccgctcg acgcatcgca
     4561 cgcatgaagt agctcttgcc ggtgccccag tcgccgaaca gaccgacggc cagcggcggt
     4621 cggacctcgg tcgatagcat gacggcggtc agcgtctgga cgtcctgctg gatgtcgagt
     4681 tcgtcggcgt cctgagcctc gtcctcctcc acggcgtcgg aatggaagcc ggccatgaac
     4741 accgggcgcg acgtgcgagc cccggtgcga cgttcgagcg ccgtgcgcag gaccgcggcg
     4801 atcgcgccat tcctcggttc gggtgctgct gcgcgcagcg cggcgtcgac ggcggtcgca
     4861 tcgagcggca ccagcacctc ggcgaaccga gcaatcgtcg tatgggcggc atcgagcccg
     4921 gcctcgatca cccggcgcac ggttccgtcg accccgaggt gctcgaacag caccggcgcg
     4981 gccgcgcgcg ccatcgccgg cgaagccagt gcatagatgg catcgtaagc agggtcgctt
     5041 tggcccgatg agctcacgag ccgcagggcg acagtggctg ccagccacgt gagcgggacg
     5101 acgcgcaata cccgcaggac cggccaggcg acctcgaagt tgtccgaggt gaggtctgcg
     5161 cggatgtcgt tttcgaacga cggttcggat gagctggcga tggcgcgagc gagcagtcgg
     5221 acctccggcg ccggatcgtt cacggcggct gcgaccgcct cgccgaggta cgcggcgcga
     5281 acctggtaca gacccgcgag ggcccagaac cgtacgttgc ggtccgactc ggtctcgtgc
     5341 gagacgtgct tcaggaccac ggctcggctc gcctcggact cgggttcgga ccagatcagg
     5401 aagctcagca tccatgcgcg gatcgacgga acgagcttgg gatcgcgaac cgctgagcca
     5461 tatgccgact cgcggcccgg tgcgaagcgc ccggtgatct cgtcgcgcag ccgcgctgcc
     5521 agcgccgcgg tatcggtgaa gctcttcgtg gtgatctgtt gcaggaggtt cgagcgctgt
     5581 tcttcgttgc cgttgatgat cccaccgatg atcgcgtcta gcgcggcggg ccccgagtgc
     5641 ctgcgtcgac gaatcgcttc ggcgaggtag tcgagctctc tcggcccggt cttgcgcaag
     5701 tcggcgtagc gctgcttctg gaagtagccg ttggcccact gcaatgattc cgcgggcggg
     5761 tcgccgatga cggcaagcag catccgccgc tgctcggtga gtgcccgatc ggcgagtcgc
     5821 tgttgctgcg gttcccagcc ccgcgcgccg aggagcacga ggatcagtgg cgccgccgtg
     5881 aactgcgcct cttcgagagc gttgaggctg tcgccgatcg ccacgtccct gcgccagtag
     5941 tgcagcgaca cgccgcgctg ggccagagca tcaacgattc ggtcgatcgc gtcctcctgc
     6001 tcgttgtaga gcaagaatgc atcgatacct gccgtagccg ggcctgctgc aaccgccata
     6061 aatgtcctcc tcctgcctcg aggctcgcga taacctaaca gaatcggctg cgtctcagtc
     6121 gcgcagacgt taccgctggc gatgctcgaa cccgaccgct accccagcgc cgcgacctgg
     6181 catggcagaa cgaacattcc gagcttcccg gctcgtaagg cacccgatca tggccagtgg
     6241 tcgggtcatc gggctgcgtg tgcggtgcgg cgggcgatca gcggaagccg tccttccagc
     6301 tgcggagcgc ggcgaacacg ctgaccggat cgccggcggc gccgcgcgcc ttcgcggcgg
     6361 cgatcaccgc gggctcggcg gcgcgcagga acgggttggt cgcgcgctcg tcggcgatcg
     6421 tcgagggggt cgacggggtg gccagcgcgc cggcgcggcg ggcgaccgcg gcgttgtcgg
     6481 gctcgaccgc ggccgcgaac cgcaggttcg tcgcggtgta ctcgtggccg aagtagaccc
     6541 gggtgtcggg cggcagcgcc gcgagccgca tcagcgaggt gtgcatcgtc gcggcgtcgc
     6601 cctcgaacag ccggccgcag ccggcgccga acagcgtgtc gccggtgaac gcgcagccgt
     6661 cgacgacgta ggtgatcgcg ccgaccgtgt ggccggggtt gtggatgcag cgcgcgcgca
     6721 aggggccgag cgcgacctcg tcgccgtcgt cgacccggcg ggtgacgtgc ggcgtcttcg
     6781 ccgcgtcctt gacgccgatc acgacctcga cgccgccgtg gcgggcgacc agctcgccga
     6841 tgccgccgac gtgatcgggg tggtggtggg tcgcccacac cgcggcgagc cgggcgtgct
     6901 cgcgcgcgag cgcggcctcg accggcgcgg cctccccggg gtcgaccacg gcgcaccggc
     6961 cgtcgtcgtc gatcacgagg taggcgaagt tgtccttgag gcacggtacc gagacgatcc
     7021 gcataccggc gttctaccgc gattcgcatc cggcgggctg ccgcgttacg gtggccggca
     7081 tgacgatgac cgcgatggtg tttcgcgagc acggaggccc cgacgtgctc cacctcgaga
     7141 cgctgcccgt gcccgagccc ggccccggcg aggtccgcgt ccgggtccgc gcggtggcgc
     7201 tcaaccacct cgacatctgg acccggcgcg gcggcccggc gttcaagctg acgttcccgc
     7261 acctgctcgg cgccgacatc gccggcgtgg tcgacgcgat cggcccgggc gcgaccgccg
     7321 cggtggggac cagggtcgtg ctgcagcccg ggctgtcgtg cggccggtgc gcggcgtgcc
     7381 tcggcggtca cgacaacctg tgccggtact actcgatcct cggcgagcac aagccgggcg
     7441 gctacgccga gtacatcgtc gtgcccgacg ccaatttggc gccgtacccc gagcggctcg
     7501 acttcgcgca cgccgcgtcg tcgatcctgc cgttcctgac cgcgtggcag atggtcgtcc
     7561 acaaggcgca ggtcgcgccc ggcgacaccg tgctggtcca cggcgccggc tcgggcatcg
     7621 gcgtcgccgc gatccagatc gccaagctgt acggcgcgcg cgtgatcgcg acggcgggca
     7681 gcgacgacaa ggtcgcgcgg ggcagggcgc tcggcgccga cgtggtcatc aactacagca
     7741 ccgcggactt cgccgccgag tgccgcacgc tgaccggcaa gcgcggcgtc gacagcgtga
     7801 tcgagcacgt cggcggcgag gtgttcgccg ccagcatcaa ggcggtccgc accggcggcc
     7861 ggatcgtgac ctgcggcgcg accgccggct tccacccgga gatcgatctg cgccacatct
     7921 tcttccgcca gatcgaggtg ctgggctcga cgatgggcag caaggccgac ctgctggcgg
     7981 tgctgtcgca cgtcgcggcc ggccggctcg agccggtggt ccatggcgtg atgccgctgg
     8041 ccgaggccgc cgcggcgcac cggctgctgg agaaccgcgc cgcgttcggc aaggtggtgc
     8101 tcgagccgtg accgcgccgg cggccaccgc tcgctagtcg cggtccggat cgtcgccgct
     8161 gtcatcgtca tcgtcgtcgg cgtcgtcggc gtcggcgacc tggatgccgt ggcgctcgac
     8221 caggcggcgc agctgcgtgc ggtgcagccc gagcgcgcgc gcggtcgccg cgacattgcc
     8281 gccgt
//
LOCUS       DASVOR010002277         1674 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_259045,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002277 DASVOR010000000
VERSION     DASVOR010002277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259045"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VF516_42135"
     CDS             complement(<1..1119)
                     /locus_tag="VF516_42135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114425.1"
                     /translation="MSSLAALGCTDDPPTPTEVRSQISSDLGSVLHETNAAFSGSTAS
                     LPGGAALAMVDQVLGTSTPASLRVRQLVAPFVAGGSNADPPSNDLIDADASVSYLDDQ
                     LFTDANHLGNGIYQVPPSLVCSQTTVDPSGNPVKTIDQACADRLAKADLRIRVARDGG
                     ALIIAIQVDADHDEPLRFTLTHTSLAVTVDLDDMQRAIAALATLFNEDLPNVALAGQV
                     TAKLEILGAAKLRASTTIDRDLSIKGAKTGASLDGPDALVLTSAKAEAFSVTLDGNAT
                     SGSFTVGLGETTMKLPAFTNNKRFELDLPGATANATFIADQPLTLTHVGLGNRTTTVS
                     LDGARAETIDINPHDGRAFDATVSRDPTTGKETITVTPK"
BASE COUNT          228 a          536 c          630 g          280 t
ORIGIN      
        1 cttcggcgtg acggtgatcg tctccttgcc ggtcgtgggg tcgcggctca ccgtcgcatc
       61 gaatgcgcgg ccgtcgtgcg ggttgatgtc gatggtctcg gcgcgggcgc cgtcgagaga
      121 caccgtcgtg gtgcggttgc cgaggccgac gtgcgtcagg gtgagcggct ggtccgcaat
      181 gaaggtcgcg ttcgcggtgg cgccgggcag gtcgagctcg aagcgcttgt tgttggtgaa
      241 cgcaggcagc ttcatggtgg tctcgccgag gcccaccgtg aacgagcccg acgtcgcatt
      301 gccgtcgagc gtgaccgaga atgcctcggc cttcgccgac gtcaggacca gcgcatcggg
      361 gccgtcgagg ctcgcgccgg tcttggcacc cttgatcgac agatcgcgat cgatcgtggt
      421 cgacgcccgc agcttcgcgg cgccgaggat ctcgagcttg gcggtgacct ggccggcgag
      481 cgccacgttg ggcaggtcct cgttgaacag cgtggcgagc gccgcgatcg cgcgctgcat
      541 gtcatcgaga tcgaccgtga ccgcgagcga ggtgtgggtc agcgtgaagc gcagcggctc
      601 gtcgtgatcg gcgtcgacct ggatcgcgat gatcagcgcg ccgccgtcgc gggcgacgcg
      661 gatccgcagg tcggccttgg cgagccggtc ggcacacgcc tggtcgatcg tcttgaccgg
      721 gttgccgctc gggtcgacgg tggtctggga gcacaccagc gacggcggga cctggtagat
      781 gccattgccc aggtgattgg catcggtgaa cagctgatcg tcgaggtagg acaccgacgc
      841 gtcggcgtcg atcagatcgt tgcttggcgg gtctgcgttc gagccgcccg cgacgaacgg
      901 cgcgaccagc tggcggacgc gcaggctcgc cggggtgctc gtgccgagca cctggtcgac
      961 catcgcgagc gctgcgccgc ccggcagcga tgccgtgctg cccgagaatg cggcgttggt
     1021 ctcgtgcagg acactgccga ggtcactcga gatctggcta cggacctcgg tcggcgtcgg
     1081 tgggtcgtcg gtgcagccga gcgcggcgag cgacgacacg gccgcgaatg cgagaagcga
     1141 agcagggagt ctgtgcatgg acttcgagca tcttccaacc atcgtgccag cgacgtttcc
     1201 cggtgggttg ccagaccttg ccgcgacacc cgtgtcagtg cgtcgcgcaa gcccgcgtgg
     1261 atccaggagc tcggcgagcc gtgatcacgg cgccgctgtg aaccgagagg tacgcctggc
     1321 gtcgaaaaca cgccaccgct gtccagggtc cgccggattt cgcggcagct cggcgcgtgc
     1381 cggacgccag ggcgtagcga gaccgtcggc gctcgccggc gcgaccggct gcactgccgt
     1441 tgcgccgggt caggagcacg gctccgcggt tcccgccctg ttccggcggg atgcgcaaag
     1501 tagccagcac gggcacctcg tgcatggcgg gggcgccgcg gagtacgctg cgcgccgtgg
     1561 aacgctcccg cctgtcgccg ttcggactgg tcctgctcgc gctggtcagc gtcgcctggg
     1621 ggctgtcgtg ggcgtcgatc aagatcgtgc tcgccgaggt cccgccgctg acgt
//
LOCUS       DASVOR010002278         3816 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_141476,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002278 DASVOR010000000
VERSION     DASVOR010002278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141476"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="VF516_42140"
     CDS             complement(<1..423)
                     /locus_tag="VF516_42140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114426.1"
                     /translation="MRWQIVYLLRDFVVAPHTFVRPRPRDTAAPPELGAKVPELPGLS
                     PGRPAVVAFLRHVGCPFAEATVRQMAALAAERPGIDLLAVTHSDEAPSRRWCESFGGA
                     GGVQLITDPDRARYAAWGVGLSDRRHFAGPESLGALRRL"
     gene            529..1338
                     /locus_tag="VF516_42145"
     CDS             529..1338
                     /locus_tag="VF516_42145"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEX8114427.1"
                     /translation="MHDDSIDPTVLDRVSEVFARARSALFITGAGISADSGLPTYRGI
                     GGLYEDADAATEEGLAIEDALSGDMLRARPEVCWKYIHQIERACRGATWNRGHEVLAL
                     MERRYERTWVLTQNVDGFHRGAGSNNVIEIHGDVHKLACTRCAYRDHVEDFAALSIPP
                     RCPDCGAIVRPEVVLFGEMLPRAAVAVLDRELRRGFDLVVSIGTTSVFPYIAAPVMLA
                     RSAGRDTVEINPGTSGVSHVVAHRIQNRAAVALDAIWRRLETGPLGAGRRP"
     gene            complement(1343..2062)
                     /locus_tag="VF516_42150"
     CDS             complement(1343..2062)
                     /locus_tag="VF516_42150"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX8114428.1"
                     /translation="MRKTVLGLAGLALGLAAAAHVLLPSAPRRVKPARPDCQVGPTTP
                     GIDVSYYQEAIDWPRVHRAGIRFAFIRLSDGATLPDARFATNWAEARRAGLARGAYQF
                     FRPDQSVATQAELMIAAMRDRQHDDLPPVIDIEVDGGLAAPVVAARARAWVDRVRAGL
                     GVEPIVYTGGDLWRNGGAELLASQPLWVAHYTQGCPTLPSPWIRWTFWQHTDRGAVPG
                     IDGAVDLDQFAGTLGELHAAR"
     gene            complement(2170..2934)
                     /locus_tag="VF516_42155"
     CDS             complement(2170..2934)
                     /locus_tag="VF516_42155"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEX8114429.1"
                     /translation="MVRIALAALAARIIASLLTSFLTPTLAHAAPGPAAAATGTVKGT
                     VVFDGPAPERPKVKRDSDPYCAKTEMLADDIIVTNGKLRDVLVRVKNGSLHAPAGAPP
                     AAPPPAVIDQKDCRYVPRVLGVVVGQKLSIHNNDNTFHNVHGEIDGKLVWNRPTGQGD
                     PPLNLDGSAHAGDVIEVKCDVHNWMHAYAVVQDHTAFAVTGDDGAFEIKGLPPGSYTL
                     EAWHPALGTRTVTVQIGKGAKATATAKFSFKPGAPK"
     gene            complement(3043..>3816)
                     /locus_tag="VF516_42160"
     CDS             complement(3043..>3816)
                     /locus_tag="VF516_42160"
                     /inference="COORDINATES: protein motif:HMM:NF039709.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6263 family protein"
                     /protein_id="HEX8114430.1"
                     /translation="PKTALRMSAKQGDHQTLVMTIAMGMGIQSDGKDIVPMTHVPAMQ
                     MTMGVDVVSVAGNGDIRCNLEISKAELLPDPNANPAVASAMKHALAGLAGLTGYTVVT
                     NRGFTKDADFTIGPNADPQLTEMLNSMRQSLRQLSSPLPDEPVGKDAQWMTTMKLQVN
                     GMAMTQVSTHRLLERSGNSAKIELSLKQSAGRQKIQRNGVTVDLFSLNSTGSGAQTFD
                     LSHVIPVAAQVSLNSVSDMEAMGQKMTLKLDVTMDVNSH"
BASE COUNT          526 a         1291 c         1437 g          562 t
ORIGIN      
        1 caggcggcgc agcgcgccga gcgactcggg gccggcgaag tggcggcgat cgctgaggcc
       61 gacgccccac gcggcgtagc gcgcgcgatc gggatcggtg atcagctgga cgccgcccgc
      121 cccgccgaac gactcgcacc accgccgcga cggcgcctcg tcgctgtggg tcacggcgag
      181 caagtcgatc cccggccgct cggcggcgag cgcggccatc tggcgcaccg tggcctcggc
      241 gaacgggcag ccgacgtggc ggagaaacgc gacgaccgcc ggacggccgg gcgagagccc
      301 ggggagctcg gggaccttgg cgccgagctc ggggggcgcc gcggtgtccc tgggcctggg
      361 ccggacgaag gtgtgagggg cgacgacgaa gtcgcgcagg aggtagacga tctgccaccg
      421 catgccgcga gcctatcgcg cgccgggccg cggcgggcga atcgccggcg cggacgctcc
      481 gggtgataag ctaccgcaca gggcgcgcgg tgacgcgctg gggcggacgt gcacgacgac
      541 tcgatcgacc cgactgtcct cgaccgcgtc tcggaggtct tcgcgcgcgc gaggagcgcc
      601 ctgttcatca ccggcgccgg gatcagcgcg gactccggat tgccgaccta ccggggcatc
      661 ggcggcctgt acgaggacgc cgacgccgcc accgaagaag gcctcgcgat cgaggacgca
      721 ctgtccggcg acatgctgcg cgcgcggccc gaggtgtgct ggaagtacat ccaccagatc
      781 gagcgggcgt gccgcggcgc gacctggaac cgcggccacg aggtgctcgc cctgatggag
      841 cgccgctacg agcggacctg ggtgctcacc cagaacgtgg acgggttcca ccgcggcgcg
      901 gggtcgaaca acgtgatcga gatccacggg gatgtccaca agctggcgtg cacgcgctgc
      961 gcgtatcgcg accacgtcga ggacttcgcc gcgctctcca ttcctccgcg ctgcccggac
     1021 tgcggggcga tcgtgcgccc ggaggtcgtc ctgttcggcg agatgctgcc gcgcgccgcg
     1081 gtcgcggtcc tcgatcgcga gctccgccgc ggcttcgacc tcgtggtctc gatcggcacg
     1141 accagcgtgt tcccctacat cgccgcgccg gtcatgctcg ctcgcagcgc ggggcgcgac
     1201 acggtcgaga tcaacccggg caccagtggc gtctcgcacg tggtcgcgca ccggatccag
     1261 aaccgggccg cggtcgccct cgatgcgatc tggcgccgcc tggagaccgg ccctttgggc
     1321 gcgggtcgcc gcccgtgacg ggtcagcgcg cggcgtggag ctcgccgagc gtgccggcga
     1381 actgatcgag atcgaccgcg ccgtcgatgc cggggaccgc gccgcgatcg gtgtgctgcc
     1441 agaacgtcca ccggatccac ggcgacggca gcgtcgggca gccctgggtg tagtgggcga
     1501 cccacagcgg ctgggacgcg agcagctcgg cgccgccgtt gcgccacaga tcgccgccgg
     1561 tgtagacgat cggctcgacg ccgaggccgg cgcgcacgcg atcgacccac gcccgggcgc
     1621 gcgcggcgac gaccggtgcg gcgaggccgc cgtcgacctc gatgtcgatc accgggggca
     1681 ggtcgtcgtg ctggcggtcg cgcatcgcgg cgatcatgag ctcggcctgg gtggcgacgc
     1741 tctggtcggg gcggaagaac tggtacgcgc cgcgggccag gccggcgcgg cgggcctcgg
     1801 cccagttggt ggcgaaccgc gcgtcgggca gcgtggcgcc gtcggacagc cggatgaagg
     1861 cgaagcggat gccggcgcgg tggacgcgcg gccagtcgat ggcctcctgg tagtaggaga
     1921 cgtcgatgcc gggggtggtc gggccgacct ggcagtcggg gcgcgccggc ttgacccggc
     1981 ggggcgccga cgggagcagc acgtgcgctg ccgccgcgag gccgagcgcg aggcctgcca
     2041 ggccgaggac ggtcttgcgc accaccgagc ggaatagtgc accacggcga gggacaggac
     2101 cagctggcat gacgcggaag cgtgcggagc cactggtaca acccgcgagc cgcgcgggct
     2161 attcaccggt cacttcggcg cgccgggctt gaacgagaac ttcgcggtcg cggtggcctt
     2221 cgcgcccttg ccgatctgga ccgtgaccgt gcgcgtgccg agcgcggggt gccaggcctc
     2281 gagcgtgtag ctgcccggcg gcaggccctt gatctcgaac gcgccgtcgt cgccggtgac
     2341 cgcgaacgcg gtgtgatcct ggaccacggc gtaggcgtgc atccagttgt ggacgtcgca
     2401 cttgacctcg atcacgtcgc cggcgtgcgc gctgccgtcg aggttgagcg gcggatcgcc
     2461 ctggccggtc ggccggttcc acaccagctt gccgtcgatc tcgccgtgca cgttgtggaa
     2521 cgtgttgtcg ttgttgtgga tcgacagctt ctggccgacg acgacaccga gcacgcgcgg
     2581 gacgtagcgg cagtccttct ggtcgatcac cgcagggggc ggcgccgccg gtggcgcccc
     2641 cgccggcgcg tgcagcgagc cgttcttgac ccggaccagc acgtcccgga gcttgccgtt
     2701 ggtgacgatg atgtcgtcgg cgagcatctc ggtcttcgcg cagtacggat cgctgtcgcg
     2761 cttgaccttg gggcgctcgg gcgcggggcc gtcgaacacc acggtgccct tgaccgtgcc
     2821 ggtcgcggcg gcggcggggc ccggcgcggc gtgggcgagg gtgggagtga ggaacgaggt
     2881 gagcaacgag gcgatgatcc gggcggcgag agcggcgagg gcgatgcgca ccatggcagc
     2941 gacaggctat cccgtcacgc gcctcgtgtc gcgccttgac cggcaggtgc cgaggtcgac
     3001 ggcatccgtc gccgacggtc tcgcctacgg agcgcgggtg gctcagtggc tgttcacgtc
     3061 catcgtcacg tcgagcttca gggtcatctt ctgacccatt gcttccatgt ccgacaccga
     3121 gttgagcgac acctgggccg ctaccgggat cacgtgcgag aggtcgaacg tctgcgcgcc
     3181 agaaccggtc gagttcagcg agaacaggtc gaccgtcacg ccgttgcgct ggatcttctg
     3241 gcggccggcg ctctgcttga gcgagagctc gatcttcgcg gagttgcccg atcgctcgag
     3301 caggcgatgt gtgctgacct gggtcatcgc catgccgttg acctgcagct tcatcgtggt
     3361 catccactgg gcgtccttgc caaccggctc gtcgggcagc ggcgagctga gctggcgcag
     3421 cgactggcgc atgctgttga gcatctcggt gagctgcgga tcggcgttcg ggccgatcgt
     3481 gaagtcggcg tccttggtga agccacggtt ggtgacgacc gtgtagccgg tcaacccggc
     3541 gagcccggcg agcgcgtgct tcatggcaga cgcgaccgcc ggattcgcgt tcggatccgg
     3601 cagcagctcg gccttgctga tctcgaggtt acaccggata tcgccgttgc cggcgacgct
     3661 gacgacgtcg acccccatcg tcatctgcat cgccggcacg tgcgtcatcg gcacgatgtc
     3721 cttgccgtcg gactggatgc ccatccccat cgcgatcgtc atgacgagcg tctggtgatc
     3781 gccctgcttc gccgacatcc gcagcgcggt cttcgg
//
LOCUS       DASVOR010002279         2362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_317055,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002279 DASVOR010000000
VERSION     DASVOR010002279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2362
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317055"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            187..2127
                     /locus_tag="VF516_42165"
     CDS             187..2127
                     /locus_tag="VF516_42165"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="condensation domain-containing protein"
                     /protein_id="HEX8114431.1"
                     /translation="MEPGEVEAALVAHPGVRQVAVQGRNDTGEMRLVAYLVLRGTPLS
                     SGELRAFLAPRLPDELIPSAFVVMSELPLTPDGKVNLKALPAPQAAPPRASETGETDE
                     ASRVENMDALERLIADVWQEMLEIKHVAAADNFFELGGQSMLAFRVITRLNELLSLRL
                     QVMDVFRFPTVSELAVSVREAQAQVGSMEKPPLLRDPGLPAETEASPSQEMLYRMNTE
                     APNLPAENQDLSMRIVGTLDVAALERALSEIVRRHDVLRSRFRRAGGRLMHSVVPWEP
                     LRLSIHEVSGGTDEERWAAAGQIHQRRWSSSFDLGAGTIKLELVRVSPREHLLIAIFH
                     HIIFDGMSYPVFFRELSALYRAFSRGEPSPLEELPFRFADHVAWQRKLAELPIGQSQL
                     AYWRKRFEGVGPMRIRGDKPRDLIDEKRARKGPPDVFPGRAVKVQLPPDVVAAVRKQA
                     VDLQVTVYTVLVAAYFLLVREYSEQDEVIMMTSVSGRDQPGAEQVLGQFNASFPLRVS
                     LTDHRLIRDVIFAIRDDAVAQRSNCDLPASYILDEYKQGARVIFNFLDLREAPLPELP
                     GAEVSLATRPSDPADVIMNDLAVFIADGEAAITAEVVCNADLFTDGGLRRVATDYLDL
                     VAWVTRNADRTVQDARARSSRP"
     gene            complement(2159..>2362)
                     /locus_tag="VF516_42170"
     CDS             complement(2159..>2362)
                     /locus_tag="VF516_42170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114432.1"
                     /translation="PHPGRQLLDLSFVLSEEDHQVWGELVYNADLFDADAAAGIAEHF
                     VVLIEHAVANPDLMIAELAMTRD"
BASE COUNT          390 a          784 c          781 g          407 t
ORIGIN      
        1 gacgccatcg cgcgggggta tctccacaac ccgcgggaga ccgcggagcg gttcattccg
       61 gatcggttcg ccaaccaacc cggagcccgg gtctatcgga ccggcgacct ggcgcgccgg
      121 ctccccagcg gtgtcctgga gttcgtcggc cggatcgatc atcagctgaa gatccgcggc
      181 taccgagtgg agcccgggga ggtcgaggct gcgctggtgg cgcacccagg tgtgcggcag
      241 gtcgccgttc agggccgcaa cgacaccggt gagatgcgac tcgtggcgta cctcgtactg
      301 cgaggcacgc cgctatcgtc tggcgagctt cgagcattcc tggctcccag gttaccggac
      361 gagctcattc ccagcgcgtt cgtcgtgatg tcggagttgc cgctcacgcc ggacggcaag
      421 gtgaacctca aggcgctgcc agcgccgcaa gccgcgcctc cacgtgcatc cgagaccggc
      481 gagaccgacg aggcgtcgcg cgtcgagaac atggacgcgc tggaacgact catcgctgac
      541 gtctggcagg agatgctcga gatcaagcac gtcgccgccg ccgacaactt cttcgagctc
      601 ggcggccagt cgatgctggc attccgggtg atcacgcggc tcaacgagct gctctcgctc
      661 cggctgcagg tcatggacgt ctttcgattt ccaacggtca gcgagctcgc ggtgagtgtc
      721 cgcgaggcgc aagcccaggt cgggtccatg gagaagccgc ccttgctgcg cgatcccggc
      781 ctcccggccg agaccgaggc gtcgccgtcc caggagatgc tgtaccgcat gaacacggag
      841 gcgccgaacc tgccggccga gaaccaggat ctctcgatgc gcatcgtcgg caccctcgac
      901 gtggccgccc tcgagcgcgc gctgagcgag atcgtgcggc gtcacgatgt gctgcgttcg
      961 cgcttccgac gtgccggcgg caggctcatg cattccgtgg ttccgtggga accgctgcga
     1021 ctatcgatcc atgaggtgtc gggcggcacc gacgaggagc gatgggccgc ggccgggcag
     1081 attcatcagc gccggtggtc gagctcgttc gatctcggcg ccgggacgat caagctcgag
     1141 ctggtgcgcg tgtcgcctcg cgaacacctc ctgatcgcga tctttcacca catcatattc
     1201 gacggtatgt cctacccggt tttcttccgg gagctgagcg ccctctaccg cgcgttctcg
     1261 cgtggcgagc catccccgct cgaggagttg cccttccggt tcgccgatca cgtggcgtgg
     1321 cagcgcaagc tggcagagct tccgatcggc cagtcccagc tcgcgtactg gcgcaagcgc
     1381 ttcgaggggg tcgggcccat gaggatccgc ggcgacaagc cgcgcgatct catcgatgag
     1441 aaacgagccc gcaaaggtcc ccccgatgtg ttccccggca gggccgtgaa agtgcagctg
     1501 ccccccgacg tcgtcgccgc ggtccggaag caggcggtcg acttgcaagt aacggtctac
     1561 accgtcctgg tcgccgcgta cttcctcctc gttcgagagt actcggaaca agacgaggtg
     1621 atcatgatga ccagcgtctc ggggcgggac cagccgggtg ccgagcaggt gctgggacag
     1681 ttcaacgcgt cgtttccact acgtgtgtcg ctgaccgatc accggctgat tcgcgatgtc
     1741 atcttcgcga tccgtgacga tgcggtggcg cagcgatcga actgcgacct gccggcgtcc
     1801 tatatcctgg acgagtacaa acagggcgct cgcgtcatct tcaactttct cgacctgcgc
     1861 gaagcgccgc tgcccgagtt gcccggtgcg gaggtgagcc tcgcgactcg gccaagcgat
     1921 cccgccgacg tgatcatgaa cgacctcgcg gtgttcatcg ccgacggaga ggccgccatc
     1981 accgctgaag ttgtttgcaa cgccgacctg ttcacggacg gaggattgcg ccgagtggcc
     2041 acggactacc tcgacctggt cgcatgggtg acgcgcaacg cggatcgaac cgttcaggac
     2101 gcccgcgcac gcagctcccg gccatgacgt gtggatcgcc actccgggtc agtccgggtc
     2161 agtcccgggt catcgcgagt tctgcgatca tcaggtccgg gttcgcgacg gcgtgctcga
     2221 tcaggacgac gaagtgctcc gcgatccctg cggccgcatc ggcgtcgaac agatcagcgt
     2281 tgtacaccag ctcgccccac acctgatggt cctcctccga caggacgaag gagaggtcga
     2341 gcagctggcg gcccggatgg gg
//
LOCUS       DASVOR010002280         4254 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_492478,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002280 DASVOR010000000
VERSION     DASVOR010002280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4254
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492478"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1645)
                     /locus_tag="VF516_42175"
     CDS             complement(53..1645)
                     /locus_tag="VF516_42175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114433.1"
                     /translation="MKWLAVLGVFGAGCAESADETDSAAQQAQSPAAPVMTVATAVHH
                     DVSAPLRDAPVMINPPSTRRAEHPIGRIPIKGPDLDDPVIQTQLGPTPLFPTVSPILN
                     FAGVGNGDYGFAPNAAPPDTNGVVGATQYVQWVNESFAVFDKTTGAIAPGFPKAGNTI
                     WAGFGGGCQTNNDGDPVVQYDKQANRWILTQFSVSTTPNLQCVAVSTTPDATGSYARY
                     SFQYANFPDYPKLGVWTDAYYISFNIFTSTFQGALACAYDRTAMLAGAPATQICFQQP
                     TSVASLLPSDLDGLTPPPVGSPNFFVTLQTTTSLNLFKFKVNSFSPASATFTGPTSIP
                     VASFATACRNGGACIPQSGTSQQLDSLSDRAMYRLAYRNIGGHETLLLAHSVSTGRKT
                     PAGVRWYELRSPLTGTFSVFQSGTFAPDTLFRWMPSMAMDKVGNIAVGYSTSSSTTLP
                     GIRAAVRAPTDAAGTLGNEFVIKNGGGSQLTSLNRWGDYSAMTVDPVDDCTLWFTSEY
                     LKANGTFNWSTQIASFKLAGCQ"
     gene            complement(2072..3685)
                     /locus_tag="VF516_42180"
     CDS             complement(2072..3685)
                     /locus_tag="VF516_42180"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEX8114434.1"
                     /translation="MAKDGRALREEAAAAVAAGKHKRALAAYLELERLEANDAQWPKR
                     AGETYRRLGNPSNAIEAFERSADRYAQNGFLVQAIAVCKVILQINPNHADTLRRLAEM
                     NERIGASQTRAASMADNNPALHENPSVAAIRGRTPTEAAARPPDDGLSRPRKESDGYR
                     RSRPSTPPNIAVGRSKSRPIALPLGAALDTVDLKTEVPAAHSRADEPGIHVIPIDEDE
                     FDQDTTLNTQRQPIPPGDPGWESGAPDDDIVTEVSSSTELDLSDIEEIPLPRPRLIGA
                     AAARALAATPLFAGMPSDALQALVANLQLVSLERGEVLFRAGEPSDALYVIAEGELSV
                     QNPGPPSAELSRLGPGAFLGEVALMTDQPRAATVVCLSPAELLRIDRHTLSRVLANHG
                     DVLRAVLRFVRDRLVDRWMRTSPLFRPFNDAQRIELAGKFKFLEIDAGTVVLGTGERP
                     DGLYIVLAGQFLVVRNGSAVAQLGPGDLIGETALLSGGAFKSDVVARGKGLALCLPAA
                     VFREVIMTHPHVLEYIGEQAEHSRRLQIL"
     gene            complement(3763..>4254)
                     /locus_tag="VF516_42185"
     CDS             complement(3763..>4254)
                     /locus_tag="VF516_42185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114435.1"
                     /translation="AGETYRRLGTPLPGVDPKVAEAFAAAERALARVHKLRSRPVLET
                     LSSSIRLKENATEDVVARYRAVAEYGGLAQIAASYRAGSLYHDLALSLLFELPPELDP
                     AVAAGLRRTLRGRAIADLKKAVTEYRTALAAPKHPDDELWRLAAETDLRDARDLLVAA
                     GEK"
BASE COUNT          596 a         1382 c         1599 g          677 t
ORIGIN      
        1 cccggccgct ggctcggctg ccgccggctc ggcccactag agcgtcagct ggctactgac
       61 agcccgcgag cttgaacgac gcgatctggg tgctccagtt gaacgtgccg ttggccttga
      121 ggtactcgct cgtgaaccac agcgtacagt cgtcgaccgg gtcgacggtc atcgcgctgt
      181 agtcacccca gcggttcagg ctggtcagct gcgatccgcc accgttcttg atgacgaact
      241 cgttcccgag cgtgccggcc gcatccgtcg gcgcgcgcac ggcggcccgg atcccgggca
      301 aggtcgtgct cgacgacgtg ctgtagccga ccgcgatgtt gccgaccttg tccatcgcca
      361 tgctgggcat ccagcggaac agcgtgtcgg gcgcgaaggt ccccgactgg aacaccgaga
      421 acgtgccggt gagcgggctg cgcagctcgt accagcgcac accggccgga gtcttgcggc
      481 cagtcgagac gctgtgggcc aggagcagcg tctcgtggcc tccgatgttg cggtaggcga
      541 ggcggtacat cgcgcgatcc gacagcgaat cgagctgctg cgaggtgccg ctctgcggga
      601 tgcacgcgcc accatttcgg cacgcggtgg cgaagctggc gaccgggatg gacgtcgggc
      661 ccgtgaaggt cgcgctggcc ggcgagaacg agttgacctt gaacttgaag aggttcagcg
      721 aggtcgtcgt ctggagggtc acgaagaagt tcggcgagcc cacgggcggc ggcgtgaggc
      781 cgtcgagatc cgacggcagg aggctcgcca ccgaggtcgg ctgctggaag cagatctggg
      841 tcgcgggggc gccggcgagc atcgcggtgc ggtcgtaggc gcatgccagc gcgccctgga
      901 acgtgctggt gaagatgttg aagctgatgt agtaggcgtc ggtccacacg ccgagcttgg
      961 gatagtccgg gaagttggcg tactggaacg agtagcgggc gtagctgccc gtcgcgtccg
     1021 gcgtcgtcga gaccgccacg cactgcaggt tcggcgtcgt gctcaccgag aactgcgtga
     1081 ggatccagcg gttcgcctgc ttgtcgtact gaacgaccgg atcgccatcg ttgttggtct
     1141 ggcagccgcc gccgaagccg gcccagatcg tgttgccggc cttcgggaag ccgggcgcga
     1201 tcgcgccggt ggtcttgtcg aacaccgcga agctctcgtt cacccactgg acgtactgcg
     1261 tggcgccgac gaccccgttg gtgtcgggtg gcgcggcgtt gggcgcgaag ccatagtcgc
     1321 cgttgcctac cccggcgaag ttgaggatgg ggctgaccgt cgggaacagc ggtgtgggcc
     1381 caagctgggt ctggatgacc gggtcgtcga gatccggtcc cttgatgggg atcctgccga
     1441 tcgggtgctc ggcgcgccgc gtggacggcg ggttgatcat caccggcgcg tcgcgcagcg
     1501 gcgccgacac gtcgtggtgg accgccgtcg ccaccgtcat cacgggcgcc gcggggctct
     1561 gtgcctgctg tgcagcggag tcggtctcgt ctgcgctctc ggcgcagccc gcaccgaaca
     1621 caccgagaac tgctaaccat ttcacgtatc gcatggaccc tcgagttggt tgacttcaga
     1681 cgtcagtatc aggcggcgag ctcgagtgga tatatgaatg attggggggt tcggttaaga
     1741 ctcgatcagg ggcgctgggc tctcgatctc gatccggggg gaaccgccgg gatcgggcga
     1801 cgtgatcggc gcacccgcag ctttgcgagg cacccgccgg ttgcacaagt acctgaacca
     1861 aacggaagcc tacacatgcc acgctctacc ctcctggctc tccgttgcag ccgttacaga
     1921 gatgagagcc gctgatactt gggcgtgttt tcatgcaacc agatgatggg cattggccac
     1981 ggaacgtgac cgggttcgca ggcgggacgc ggccgcccga agccggatgt ccggccgggg
     2041 cctgggtcca tctcgatctt gcagagtggt tctacaggat ctgcaggcgg cggctgtgct
     2101 cggcctgctc gccgatgtac tcgaggacgt gcgggtgggt catgatgacc tcgcggaaca
     2161 ccgcggcggg caggcacagc gcgaggccct tgccgcgggc gaccacgtcg ctcttgaacg
     2221 cgccgccgga tagcagcgcg gtctcgccga tcaggtcgcc ggggccgagc tgggcgaccg
     2281 ccgagccgtt gcgcacgacg aggaactggc cggcgagcac gatgtagagc ccgtcgggcc
     2341 gctcgccggt gccgagcacg acggtgccgg cgtcgatctc gaggaacttg aacttgccgg
     2401 cgagctcgat gcgctgggcg tcgttgaacg gccggaacag cgggctggtc cgcatccagc
     2461 gatcgaccag gcgatcgcgg acgaaccgca gcaccgcgcg cagcacatcg ccgtggttgg
     2521 cgagcacgcg cgacagcgtg tggcgatcga tgcgcagtag ctcggcgggc gacaggcaga
     2581 ccacggtggc cgcgcgcggc tggtcggtca tcagcgcgac ctcgccgagg aacgcgccgg
     2641 gccccaggcg cgacagctcc gcgctcggcg ggcccgggtt ctggaccgac agctcgccct
     2701 cggcgatgac gtacagcgca tcgctcggct cgccggcgcg gaacaggacc tcgccgcgct
     2761 ccagcgacac cagctggagg ttggcgacca gggcctggag cgcgtcgctc ggcatgccgg
     2821 cgaacagcgg ggtggcggcg agcgcgcgcg ccgcggcggc gccgatcaag cgcggccgcg
     2881 gcagcgggat ctcctcgatg tcggacaggt cgagctcggt cgagctggag acctcggtca
     2941 cgatgtcgtc gtcgggcgcg ccgctctccc agcccggatc gcccggcggg atcggctggc
     3001 gctgggtgtt gagggtggtg tcctgatcga actcgtcctc gtcgatcggg atgacgtgga
     3061 tgccgggctc gtccgcgcgg gagtgcgcgg ccgggacctc ggtcttgagg tccaccgtgt
     3121 cgagcgcggc gccgagcggc agcgcgatcg gccggctctt gctccgtccg accgcgatgt
     3181 tgggcggggt cgagggccgg gagcggcgat agccgtcgga ctccttgcgc ggccgcgaca
     3241 ggccgtcgtc gggcgggcgc gccgccgctt cggtcggggt ccgaccgcgg atcgccgcga
     3301 cgctcgggtt ctcgtgcagc gccgggttgt tgtcggccat gctcgcggcg cgggtctggc
     3361 tggcgccgat ccgctcgttc atctcggcga gccggcgcag cgtgtcggcg tggttggggt
     3421 tgatctggag gatgaccttg cacaccgcga tcgcctggac gaggaagccg ttctgggcgt
     3481 agcggtccgc cgagcgctcg aaggcctcga tcgcgttgga cgggttgccg agccgccggt
     3541 aggtctcgcc ggcgcgcttg ggccactgcg cgtcgttggc ctcgagccgc tcgagctcga
     3601 ggtaggccgc cagcgcgcgc ttgtgcttgc cggcggcgac cgccgccgcg gcctcctcgc
     3661 gcaatgctcg accgtccttg gccatctgcg acgcagacta tagatcagaa cgcgacctgg
     3721 ccaagcgcgc cgtcgaggcc gccgcgcgcc gcatcgcgcg ggtcatttct cgcctgcggc
     3781 gaccagcagg tcgcgggcgt cgcggaggtc ggtctcggcg gcgagccgcc acagctcgtc
     3841 gtcgggatgt ttcggagccg ccagcgcggt gcggtactcg gtgaccgcct tcttgagatc
     3901 ggcgatggcg cggccgcgca acgtgcggcg cagcccggcg gcgaccgcgg ggtcgagctc
     3961 gggcggcagc tcgaacagca ggctcagcgc gagatcgtgg tacaggctgc cggcgcggta
     4021 gctcgccgcg atctgcgcca gcccgccgta ctcggcgacg gcgcggtagc gggcgacgac
     4081 gtcctcggtg gcgttctcct tgagccggat cgagctcgac agggtctcga gcacgggccg
     4141 cgagcgcagc ttgtggaccc gcgcgagcgc gcgctccgcg gcggcgaacg cctcggcgac
     4201 cttgggatcg accccgggca gcggggtgcc gagccgccgg taggtctcgc cggc
//
LOCUS       DASVOR010002281         2033 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_170653,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002281 DASVOR010000000
VERSION     DASVOR010002281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170653"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VF516_42190"
     CDS             <1..573
                     /locus_tag="VF516_42190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8114436.1"
                     /translation="QVGRCRAILEQMAQGAGTVGEGVASCTIDELLDEALVGARSAPP
                     VHREVPAELAQVGLRLPPRAVSQALRSLITNAQDASPPTSAVMISVQSEPSRIELAIR
                     DRGAGMPDEILARIGEPFFTTKPPGRGMGLGLFLARAVFEAVGGGLDIESVTGAGTCV
                     RVTLPTDVAQGVEIRRSRESTPSGMVSKPA"
     gene            570..1127
                     /locus_tag="VF516_42195"
     CDS             570..1127
                     /locus_tag="VF516_42195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8114437.1"
                     /translation="MTSEHKPSILIVDDDETYRNRLARAFVDRGYDVRTAQDYDSALA
                     AATSESPEFAVVDLKMPGKSGLELVKSLHEIDPATKSVVLTGYGSIATAIDAVRLGAA
                     YYLSKPADADDIVGAFARAEAPPLAPPAPDSDEYKAPSLARAEWEHINRVLSDAGGNI
                     SEAARRLGIHRRSLQRKLQKYPPRE"
     gene            complement(1233..>2033)
                     /locus_tag="VF516_42200"
     CDS             complement(1233..>2033)
                     /locus_tag="VF516_42200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114438.1"
                     /translation="QGPRAQIAAALAAGRHVRIDGPRGPVHVWIPASYHADTGATVIY
                     VHGYFDDADTAWTGHQLAQQFALSALNALFVVPEAPVAQKTPINYPDLGELLRLVEDA
                     TGVPRGAALTAAVGHSGAFRTLQAWFDEPLLDQLVMVDAMYGDEDAVVGWLRASPRHR
                     LIFVGEDTLLATESVADKLPETLTVDRFPPTYDTWPAAARTARSVYVRAQYMHMPLVT
                     EGIVLPSLLRLLPVELLADEPWRLPLGSLPPLPDAAVDAADPGDAAAE"
BASE COUNT          287 a          741 c          753 g          252 t
ORIGIN      
        1 caggtcggcc gctgccgcgc gatcctcgag cagatggcgc agggcgccgg cacggtcggc
       61 gagggcgtgg cgtcgtgcac gatcgacgag ctgctcgacg aggcgctggt cggtgcgcgc
      121 agcgcgccgc cggtccaccg cgaggtcccc gccgagctcg cccaggtcgg gctgcggctg
      181 ccaccgcgcg cggtcagcca ggcgctgcgc tcgctgatca ccaacgcgca ggatgcctcg
      241 ccgccgacct cggccgtgat gatctcggtc cagagcgagc cgtcgcggat cgagctcgcg
      301 atccgcgatc gcggcgccgg catgccggac gagatcctcg cgcggatcgg cgagccgttc
      361 ttcaccacca agccgcccgg ccgcggcatg gggctcgggt tattcctcgc ccgcgcggtg
      421 ttcgaggcgg tcggcggcgg cctggacatc gagtcggtca ccggtgccgg cacgtgcgtc
      481 cgggtcacgc tgccgaccga cgtggcgcag ggcgtcgaga tccgacgatc gcgtgaatcg
      541 accccctccg ggatggtatc caagccagca tgaccagcga gcacaagccg tcgatcctga
      601 tcgtcgatga tgacgagacc tatcgcaacc ggctcgcccg ggcgttcgtc gatcgcggct
      661 acgacgtgcg gaccgcgcag gactacgaca gcgcgctcgc cgcggcgacc agcgagtcgc
      721 ccgagttcgc cgtggtcgac ctcaagatgc cgggcaagag cggcctcgag ctggtcaagt
      781 cgctgcacga gatcgatccc gcgaccaaga gcgtggtgct caccggctac ggcagcatcg
      841 ccaccgcgat cgacgcggtc cggctcggcg ccgcgtacta cctgtccaag ccggccgacg
      901 ccgacgacat cgtcggcgcg ttcgcgcgcg ccgaggcccc gccgctcgcg cccccggcgc
      961 cggactccga cgagtacaag gcgccgtcgc tggcccgcgc cgagtgggag cacatcaacc
     1021 gcgtgctgtc ggacgccggc ggcaacatct ccgaggccgc gcgccggctc ggcatccacc
     1081 gccgcagcct gcagcgcaag ctccagaagt acccgccgcg cgagtgatgg atgagccgcg
     1141 cgacgcgacg cgcggagggg tgaatggccc ggctttgccg ggccagccgc cagccgacgg
     1201 gaagatcgtc ccctgtcgat cgacgccggg ggtcactccg ccgccgcgtc gccggggtcg
     1261 gccgcgtcga ccgcggcatc cggcagcggc ggcagcgagc cgagcggcag ccgccacggc
     1321 tcgtcggcga gcagctcgac cggcagcagg cgcaacagcg acggcagcac gatgccctcg
     1381 gtcaccagcg gcatgtgcat gtactgggcg cggacgtaga ccgagcgcgc ggtccgcgcc
     1441 gcggccggcc aggtgtcgta ggtcggcggg aaccgatcga cggtcagggt ctcgggcagc
     1501 ttgtcggcga ccgactcggt ggcgagcagg gtgtcctcgc cgacgaagat caggcggtgg
     1561 cgcggcgacg cgcgcagcca gccgaccacc gcgtcctcgt cgccgtacat cgcgtcgacc
     1621 atcaccagct ggtcgagcag cggctcgtcg aaccacgcct gcagggtgcg gaacgcgccg
     1681 gagtggccga ccgccgcggt cagcgccgcg ccgcggggca cgccggtcgc gtcctcgacc
     1741 agccgcagca gctcgccgag atccgggtag ttgatcggcg tcttctgcgc gaccggcgcc
     1801 tcggggacca cgaacagcgc gttgagcgcc gacagcgcga actgctgcgc gagctggtgg
     1861 ccggtccacg cggtgtcggc gtcgtcgaag tagccgtgga cgtagatcac cgtcgcgccg
     1921 gtgtcggcgt ggtagctcgc cgggatccag acgtggaccg gcccgcgcgg cccgtcgatc
     1981 cggacgtggc ggccggcggc gagggcggcg gcgatctgcg ccctggggcc ctg
//
LOCUS       DASVOR010002282         2333 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_403714,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002282 DASVOR010000000
VERSION     DASVOR010002282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2333
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403714"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="VF516_42205"
     CDS             <1..464
                     /locus_tag="VF516_42205"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEX8114439.1"
                     /translation="WGPMVALEVDSELVAGALALPALGRTYWAARGAGAWRDGTRLAV
                     SAIADLADATLSIGELRRMATHPGIQGLIGRAASTRCYGDLAGCAMVLDGRAEVWIEG
                     GVQPWDVAAPAILIREAGGRYTDWSGGPSFESGYAIASNGLVHDEILRAMA"
     gene            713..2014
                     /locus_tag="VF516_42210"
     CDS             713..2014
                     /locus_tag="VF516_42210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114440.1"
                     /translation="MAVPVDSDPLSGLPTGAAQWTAVCAKHYGDAISAKFCAGTAPPA
                     LTSLADLEALLGLTVQPNPNNDPTINANVRFTLAGESTGLGMRSVNPVLPRAFLMTPA
                     TGTSPNPSYQVLAFARGEPLVELVASDAKAQTLRFFLIRFHPACEATRCGNADLQGAA
                     IESGWTGYTLYDDATIADTTLDCLSCHQPGGPSTKKILRMQELANPWAHWFYPERPAT
                     LQIVQDFLAAHGSESYAGIPSALVMPSRPFALMTLAQNNGFATQPNAFNSLQINNELA
                     ASGSSATWVALYAKAVAGQEIPPPYFTNPFDPTKEQAAIAAHQQLLAGTLPAAQLPDL
                     RDVFADTALAGMSIHPQAGLDGKGILVHMCQMCHNSQLDPSLSRARFNVEQLAQLSRE
                     EKDLAILRLQLPPDDRHLMPPARFHELSAAERQLAIAELMK"
     gene            complement(2030..>2333)
                     /locus_tag="VF516_42215"
     CDS             complement(2030..>2333)
                     /locus_tag="VF516_42215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114441.1"
                     /translation="AILGGAFAYAACDWGRWPHLIYLPIDGRLVLASSSAVAIHYLGL
                     VAWGLGGACAGAMLGAVLCRVRSAPWPAAVYRLLGAWAITAILLAGGYFTWSLWPW"
BASE COUNT          340 a          820 c          871 g          302 t
ORIGIN      
        1 tgtgggggcc gatggtcgcg ctggaggtcg acagcgagct ggtcgccggg gcgctcgcgc
       61 tgccggcgct cggccgcacc tactgggcgg cgcggggcgc gggggcctgg cgcgacggca
      121 cccggctcgc ggtctccgcg atcgcggatc tcgcggacgc gacgctgtcg atcggcgagc
      181 tgcggcggat ggcgacccac cccggcatcc aggggctgat cgggcgagcg gcgtcgaccc
      241 ggtgctacgg cgatctcgcg ggctgcgcga tggtgctcga cgggcgcgcc gaggtctgga
      301 tcgagggcgg cgtgcagccg tgggacgtgg cggcgccggc gatcctgatc cgcgaggccg
      361 gcggccgcta caccgactgg agcggcgggc cgtcgttcga gtcgggctac gcgatcgcgt
      421 cgaacggcct ggtccacgac gagatcctgc gcgcgatggc ctgagcgcga tcgagcggcg
      481 gcagcgcgac gggtgtgcaa ggctcccaca ggtaacgcgc ccccggcgct gccaacgccg
      541 ttgctgtgga tcgctgtcct cagggggtgc agcggtgcgg ggttagcgat ggactcgccg
      601 ttgcacagtc gacgcgacca tgcgccaact tgcttgcctg ttgctcgttt catggatcgc
      661 ggggtgtggg gcggatgccc ggggcggggg tgacccgggg cccgatgccg ggatggccgt
      721 gccggtcgac tcggacccgc tgagcggcct gccgaccggc gcggcgcagt ggaccgccgt
      781 gtgcgcgaag cactacggcg atgcgatcag cgcgaagttc tgcgccggga ccgcgccgcc
      841 ggcgctgacg tcgctggccg atctcgaggc gctgctcggg ctgaccgtcc agcccaaccc
      901 gaacaacgat ccgacgatca acgccaacgt gcggttcacg ctggccggcg agtcgaccgg
      961 gctcggcatg cgctcggtca acccggtgct gccgcgcgcg ttcctgatga cgccggcgac
     1021 cggcacgtcg cccaacccga gctaccaggt gctggcgttc gcgcgcggcg agccgctggt
     1081 cgagctggtc gccagcgatg ccaaggcgca gacgctgcgg ttcttcctga tccggttcca
     1141 cccggcgtgc gaggcgaccc gctgcggcaa cgccgacctg cagggcgcgg cgatcgagtc
     1201 gggctggacc ggctacacgc tgtacgacga tgcgacgatc gccgacacga cgctcgactg
     1261 cctgagctgc caccagccgg gcgggccgtc gacgaagaag atcctgcgca tgcaggagct
     1321 cgccaacccg tgggcgcact ggttctaccc cgagcggccg gcgacgctgc agatcgtcca
     1381 ggacttcctg gccgcgcacg gcagcgagag ctacgccggg atcccgtcgg cgctggtgat
     1441 gccgagccgg ccgttcgcgc tgatgacgct ggcccagaac aacggctttg ccacccagcc
     1501 caacgcgttc aactcgctgc agatcaacaa cgagctcgcg gcgagcggat cgagcgcgac
     1561 gtgggtggcg ctgtacgcca aggcggtcgc cgggcaggag atcccgccgc cgtacttcac
     1621 caacccgttc gatccgacca aggagcaggc ggcgatcgcg gcgcaccagc agctgctggc
     1681 ggggacgctg ccggccgcgc agctgcccga tctgcgggat gtgttcgcgg acaccgcgct
     1741 ggccggcatg tcgatccacc cgcaggccgg tctcgacggc aagggcatcc tcgtccacat
     1801 gtgccagatg tgccacaaca gccagctcga tccgtcgctg tcgcgggcgc ggttcaacgt
     1861 cgagcagctc gcgcagctgt cgcgcgagga gaaggacctc gcgatcctgc ggctgcagct
     1921 gccgcccgac gaccggcacc tgatgccgcc ggcgcggttc cacgagctgt cggcggccga
     1981 gcgccagctc gcgatcgccg agctgatgaa gtagcgggcc ggtacacgct caccacggcc
     2041 acaggctcca ggtgaaataa ccgccggcga gcaggatcgc ggtgatcgcc caggcgccga
     2101 gcagccggta gaccgccgcg ggccacggcg cggaccgcac gcggcacagg acggcgccca
     2161 gcatggcgcc ggcgcacgcg ccgccgagcc cccacgcgac caggccgagg tagtggatcg
     2221 cgaccgccga gctcgatgcg agcacgagcc ggccgtcgat cggcaggtag atcaggtgcg
     2281 gccagcgccc ccagtcgcac gcggcgtagg cgaacgcgcc gccgaggatc gcg
//
LOCUS       DASVOR010002283         5164 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113349,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002283 DASVOR010000000
VERSION     DASVOR010002283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113349"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /locus_tag="VF516_42220"
     CDS             complement(<1..364)
                     /locus_tag="VF516_42220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8114442.1"
                     /translation="MGGGFDREPATSLARDEVSKAGTPVAGKRTLTEQLPVQRSANGT
                     GTTDGRAVQEAATQGIAGGGGPLPHADTIQRLFGRHDISGVEAHVGGPAATASRAIGA
                     EAYATGHHVAFAAAPSLHT"
     gene            995..1600
                     /locus_tag="VF516_42225"
     CDS             995..1600
                     /locus_tag="VF516_42225"
                     /inference="COORDINATES: protein motif:HMM:NF024734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEX8114443.1"
                     /translation="MGARAFEREVELFRRHGGGLRMSEALRLGINRKTLYAMRDVGVL
                     EPVTRGLYRLASLEPLAHPDLVLVARRVPQGVLCLISALSFHELTTQVPHAIDVALER
                     GTRKPRIDYPPTRFFWFSGPAFHEGIETHTLDHVSVRIYDPEKTLADCFRYRNQLGMD
                     VVLEALRLWRERRRKKLDVLLKYARIRHVERGMRPYLEAMP"
     gene            complement(1579..2376)
                     /locus_tag="VF516_42230"
     CDS             complement(1579..2376)
                     /locus_tag="VF516_42230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114444.1"
                     /translation="MFGSFVSERGPLHERRRLLGEVRSERDRRHVDRCLAVGERGGDR
                     LRQQRAVEGELIREEPDVVEVLHAAVQPAQVDHRLELLGHHRLARVARELGRREAEQR
                     GVRDGLRTGSDDVAEPDVDLHGDARPAEQTDEANPHALVVRVLLDALGFHCGRVEHHA
                     VWLDIGRAHLTDHAFQVLERVVEAAEEVEVLRRARERRVPHVQHQRALEDQARPVRRL
                     RQPVEEPLHRVVLQQLLEGPLRRTRVILETRLHGGFEVLDGHGIASR"
     gene            complement(2537..3535)
                     /locus_tag="VF516_42235"
     CDS             complement(2537..3535)
                     /locus_tag="VF516_42235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114445.1"
                     /translation="MLSGKRWESGDGKGLDSAELKKTALTIKMLLEQGDGMSKPIIAD
                     EIERYLRTGDADMDGWAWPGDFMERGRRQHADLRGALVEEVRRLAKGRSHEPVPANVG
                     VEFTRAKVEPMVRGLFSKAEQDVVLATFEKSVVYVTTDTIEPILLNHMWDRSAWDLAN
                     LYLLSVGAKLLGKNAPRIVGLSEETTCYVSPDYFAEDDPFADFIVHEAAHIFHNCKRR
                     TIGLREIRRKEWLLEIEFAQRETFAYSCEAYARIVARAKGPAERRELAAEYGSKRPIS
                     AERVDPAEVASIVAEAANARNGWKVILARCAPTTKPRSIAQLARELIAARDLGDPM"
     gene            complement(3623..4534)
                     /locus_tag="VF516_42240"
     CDS             complement(3623..4534)
                     /locus_tag="VF516_42240"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEX8114446.1"
                     /translation="MPLPTFDVVISPLFELLAGNPAGLRRGEAVERLASEFSLTEEER
                     NATMGGSGKLAFMNIVDWAHGKLKWAGLSSAPSRGRWQLTAEGMTRHAEGPLTSDELA
                     ELVRGRVGAAPARDAEAEARADAQPATPDARIREAVKEIRADVELGVLERAAQLRPAA
                     FERLVIQLLVAMGYAARGDWQHTGRPGDGGVDGIILMDRLGLDRIYIQAKRLSPRTSL
                     SPDDVRAFFGALQRRRAPRGVLCTSGRVTDGMRAEAEHMGNVRLIAGDELARLMVEFG
                     LGAQTEQLRLPRLDEGFFEDFGLGGAE"
     gene            complement(4928..4999)
                     /locus_tag="VF516_42245"
     tRNA            complement(4928..4999)
                     /locus_tag="VF516_42245"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4965..4967),aa:Asn,seq:gtt)
BASE COUNT          790 a         1739 c         1716 g          919 t
ORIGIN      
        1 ccgtgtggag gctgggcgca gcggcgaacg cgacgtgatg cccggtcgcg taggcctcgg
       61 cgccgatcgc ccggctcgcg gtcgccgcgg ggccgccgac gtgggcctcg acgccgctga
      121 tgtcgtggcg gccgaacagg cgctggatcg tgtcagcgtg cggcagcggg ccaccgccgc
      181 cggcgatgcc ctgggtggca gcttcctgga ccgcgcggcc gtcggtcgtg ccggtgccgt
      241 tcgcggaacg ctgcaccggc agctgctcgg tcagcgttcg cttgccggcg acgggtgtgc
      301 cggccttgct gacttcatcg cgtgcaaggc tggtcgctgg ttctctgtcg aatccaccgc
      361 ccatggcgtg ttcctctaat ggttgcaata gcttgggtga cacctgtgtc gccgctgtcc
      421 gccgcgtcgc gtcgacggcc ttccccccgc gggtgcacgt acatggtacg gcgtccgccc
      481 ggccaggctc ccggctgccg cgtcgatttg gcggatgggc tcaagaattc gcaaccccgg
      541 tcacggcgcc tgcccggtgc cgtacgaggt tcaccgagcg gtccggcctg tgaggtcggt
      601 ggcgaagaga atcgagacaa gcccgtgtta cctgagtgaa atcctcttgc agtcgcggat
      661 cggcagaaga gctctctgcg ggccgcccgg gtggctcctg ccgctgcgcc gagcttgtaa
      721 accgccgagg tcgccgtccg gtggcacgat ccgcttcttt ccgcggttgg aggtgatgac
      781 gacggaccag gcgcgatcgc gctcggtcag gatctcgtgg ggggcgccgt tcgtagcgtc
      841 cccggctcga gttcggaggg gccaggttcc caagcgcggc gtcgccgcgt cggagggggc
      901 gggcccgagc cgttcacctg cccgctttcg agcgcgctga gccctgggga caagttgcct
      961 tcactttcta gaagtttgaa ggcaatatgt ccccatgggc gccagagcct tcgagcgtga
     1021 ggtcgagctg ttccgccgac atgggggcgg cctccgcatg tccgaagcgc tgcgcctcgg
     1081 catcaaccgg aagacgctct acgccatgcg cgacgtcggc gtcctcgagc ccgtcacccg
     1141 agggctttac cggctggcgt cgctcgagcc gctcgcgcac cccgacctcg tcctggtcgc
     1201 gaggcgggtc ccgcaaggcg tcctctgcct catctcggcg ctctcgtttc acgaactgac
     1261 gacgcaggtg ccgcacgcca tcgacgtggc gctcgagcgc gggacgcgga agccgcgcat
     1321 cgactacccg ccgacccgct tcttctggtt ctcgggcccc gcgttccacg aaggcatcga
     1381 gacgcacacg ctcgatcatg tgtccgtccg catctacgac ccggagaaga cgctcgccga
     1441 ctgcttccgc taccggaacc agctcgggat ggacgtcgtg ctggaggcgc tgcgcctctg
     1501 gcgcgagcgc cgccgcaaga agctcgatgt cctgctgaag tacgcccgca tacgccacgt
     1561 cgagcgcggg atgcgaccct acctggaggc gatgccgtga ccgtcaagaa cctcgaagcc
     1621 tccgtgcagg cgcgtctcca gaatcacgcg cgtgcgacga agcggccctt ccaggagctg
     1681 ctgcagtact acgcgatgga gcggttcctc taccggctgt cgaagacgcc gcaccgggcg
     1741 cgcttggtcc tcaagggcgc gctgatgctg cacgtgtggg acgcgccgct cgcgcgcgcg
     1801 acgaaggacc tcgacttcct cggccgcctc gacaactcgc tcgagaacct ggaacgcgtg
     1861 gtccgtgagg tgtgcgcggc cgatgtcgag ccagacggca tggtgttcga cccggccaca
     1921 gtgaaaaccg agcgcatcaa ggaggacgcg gactacgagg gcgtgcgggt tcgcttcgtc
     1981 ggtctgctcg gcagggcgcg cgtcgccatg cagatcgacg tcgggttcgg cgacgtcgtc
     2041 actcccggtg cggagaccat cacgtacccc gcgctgctcg gcttcccggc gcccgagctc
     2101 tcgggctacc cgcgcgagac ggtggtggcc gagaagttcc aggcgatggt ctacctgcgc
     2161 aggctgaaca gccgcatgaa ggacttctac gacgtctggc tcctcgcgaa tcagttcacc
     2221 ttcgacggcg cgctgctggc gaaggcggtc gccgccacgt tcgccaaccg cgagacagcg
     2281 atcgacgtgg cgccgatcgc gttcactccg gacttcaccg agcaggcgtc gacgctcgtg
     2341 cagtggaccg cgttccgaaa caaagctccc gaacaccgag tgcccggcga agctctccga
     2401 agtcgtgacg ttcctcgccg agttcctgct tccgatcgcg cgcgcctgcg aaagcggcga
     2461 gagcttccac cagcattggg taccgggcgg cccgtggacc accggcgcgt gacccgcccg
     2521 atcgcctcgg ggctcgtcac atggggtcgc cgaggtcgcg ggccgcgatc aactcgcgcg
     2581 cgagctgcgc gatcgagcgc ggcttcgtgg tcggcgcgca tcgcgcgagg atcaccttcc
     2641 agccattccg cgcgttcgcc gcctccgcga cgatgctcgc cacctccgct gggtcgacgc
     2701 gctccgccga gatcggccgc ttcgagccgt actcggccgc cagctcccgc cgctcagcgg
     2761 ggcccttggc gcgggcgacg atgcgggcgt aggcctcgca cgagtacgcg aacgtctcgc
     2821 gctgcgcgaa ctcgatttcg agcaaccatt cctttcggcg tatctcgcgc agcccgatcg
     2881 tccgtcgctt gcagttgtgg aagatgtgcg cggcctcgtg gacgatgaag tcggcgaacg
     2941 ggtcgtcctc ggcgaagtag tcaggcgaga cgtagcaggt cgtctcctcg ctgagcccga
     3001 cgatgcgcgg ggcgttcttg ccgagcagct tcgcgccgac gctgaggagg tacaggttcg
     3061 cgaggtccca tgcggagcga tcccacatgt ggttcaggag gatcggctcg atggtgtcgg
     3121 tcgtgacgta gacgacggac ttctcgaacg tcgcgagcac gacgtcctgc tcggccttcg
     3181 agaatagccc gcgcaccatc ggctcgacct tggcgcgcgt gaactcgacg ccgacgttcg
     3241 cgggcaccgg ctcgtgcgag cgccccttcg cgagccgccg gacctcctcg acgagggctc
     3301 cgcgcagatc ggcgtgctgg cggcgccccc gctccatgaa gtcaccgggc cacgcccagc
     3361 catccatgtc ggcgtcgccg gttcggaggt agcgctcgat ctcgtcggcg atgatgggct
     3421 ttgacatgcc gtccccttgc tcgaggagca tcttgatggt gagcgcggtc ttcttcagct
     3481 ccgccgagtc aaggcccttg ccgtccccgc tctcccatcg ttttccggat agcacgcgac
     3541 cgtgagaacg ggcggtcctt gctccgggag ttcggaccgg aggctggtta acagcctccg
     3601 tgagcattcc tcaaaacatt gctcattctg cgcccccgag gccgaagtct tcgaagaacc
     3661 cttcgtccag gcggggcagg cggagctgct ccgtttgtgc gcccagcccg aactcgacca
     3721 tgagccgcgc cagctcgtct ccggcaatca gcctgacatt ccccatgtgt tctgcctcgg
     3781 cccgcattcc atccgtgact cgcccggagg tgcagagcac cccgcgaggg gcgcggcgtc
     3841 gctggagagc accgaagaat gcacggacat cgtccgggga gagcgaagtt ctcggtgata
     3901 accgttttgc ctggatgtag atgcggtcga ggccgagccg gtccatcagg atgatgccgt
     3961 ccacgccgcc atcgcctggt ctaccggtat gttgccaatc tcctcgcgct gcatatccca
     4021 tggcaaccag cagctgaatg accagccgtt cgaacgccgc cgggcggagc tgcgcagcac
     4081 gttcgaggac cccgagctcc acgtcggcgc ggatctcctt caccgcctct cggatacgag
     4141 cgtccggcgt cgctggttgc gcatccgctc tggcctcggc ctccgcatct cgcgccggtg
     4201 ccgcgccgac cctgccgcgc accagttcgg cgagctcgtc gctggtcaga ggcccttccg
     4261 catggcgtgt catcccttcg gccgtgagtt gccaccggcc gcgactcggt gcagacgaca
     4321 accctgccca tttaagcttg ccgtgggccc agtcgacgat gttcatgaat gcgagctttc
     4381 cgctgccgcc catggttgca ttgcgctcct cctcggtgag cgagaactcg ctggcgagcc
     4441 tttccactgc ttcaccccgc cggagaccag cgggatttcc tgcgagcaac tcgaaaagcg
     4501 gcgaaatcac tacatcaaag gtcggtagtg gcatccgccg cgatgttgct ctgacagggc
     4561 tgaatataca agaataatac tggcttcatg gagtccgact cgatatgccg tgttggcata
     4621 tgctgcaggt tgctccgggt ataccgttgt gttcctcgct gccgatgttt ggcaacctac
     4681 ggcactccga taccatccgc tcatgtaagg cccacagccg gacctgctga ggtgagcgga
     4741 agcgagcgag gtggtgttgc gtgtccccgc gcttcgtctg gctgctgcga gatgccgctg
     4801 ggatcctggc aagtggcggc agcaaccaga ccggaagctg cttcgcatcc gggtacgcgt
     4861 ggcagccacg tggcagcaag actgttcgct gtccaagaaa gtttagttat tctggatgtt
     4921 tagaaagctc cggaggaggg tttcgaaccc ccgacccggc ggttaacagc cgcctgctct
     4981 accactgagc tactccggaa aagcgatgag ggacaaatag atgacgtacg tggggtaggg
     5041 gtgtcaagcg accgaggccg gaagcaaggc cctcgaggct gcttcgaccg cggggaggcg
     5101 aagcctcgac cggcggaaga tcgggctcag gcgctcggtg ccgcgggcgg cggcggtagt
     5161 ggtg
//
LOCUS       DASVOR010002284         1555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_141525,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002284 DASVOR010000000
VERSION     DASVOR010002284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1555
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141525"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1555)
                     /locus_tag="VF516_42250"
     CDS             complement(<1..>1555)
                     /locus_tag="VF516_42250"
                     /inference="COORDINATES: protein motif:HMM:NF024574.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114447.1"
                     /translation="EEAKVAPAGPDKAAILLELADAYGKLNNDNQVMTALSSALAQDP
                     SRLEVYDRLSALYEAKKRWPDLVKVLLEKAERVELVDKIALTLQVANLYLERFSNQAE
                     AIKAFERVLELDPDNKQAVDHLLAVYEKRRDWEKLIKLKESEVERAPDAERAAKVIEV
                     ARMAATKVKKPEICTYWWEKVLQVEPTHEEALAELYKLYERNKEWDKLAEVCSRQADA
                     ASDDKLRADALQRLGLLYTEKVENSSKAIEAWQRLLRIDENNRRAQDALKKLYVTEAR
                     WTDLEEFYRSRGKLDEFIRVLEREVEAGSESHRLELAMKIAVLYRDELQKNDRAMRAF
                     EKVLALDEDNLDAAEALIPLYEAGRDPRALVRVLEIQLRATPKGDTATRQDRIKRLAQ
                     YNEDKLRDKGAAFGWWLQAHAEDHEADSIRAELERLAGETNGWGQLVDAYAQSLPKFG
                     HKAEALPLMLVMAGVIEREQGDVDRALDMNRQILAIDDSNEQALDALERLYLGKGQFE
                     DLLGIYEKKL"
BASE COUNT          188 a          547 c          517 g          303 t
ORIGIN      
        1 gagcttcttc tcgtagatcc cgagcaggtc ctcgaactgg cccttgccga ggtacagccg
       61 ctcgagggcg tcgagcgcct gctcgttgct gtcgtcgatc gccaggatct ggcggttcat
      121 gtcgagcgcg cggtcgacgt caccctgctc gcgctcgatc acgccggcca tcaccagcat
      181 cagcggcagc gcctcggcct tgtgcccgaa cttcggcagc gactgggcgt aggcgtcgac
      241 cagctggccc cagccattgg tctccccggc gagccgctcg agctcggcgc ggatcgagtc
      301 ggcctcgtgg tcctcggcgt gggcttgcag ccaccagccg aacgccgcgc ccttgtcgcg
      361 cagcttgtcc tcgttgtact gggcgagccg cttgatccgg tcctggcggg tcgccgtgtc
      421 gcccttgggg gtcgcgcgca gctggatctc gagcacgcgg accagcgcgc gcggatcgcg
      481 gccggcctcg tacagcggga tcagcgcctc ggcggcatcg aggttgtcct cgtcgagcgc
      541 cagcaccttc tcgaacgccc gcatcgcgcg gtcgttcttc tgcagctcgt cgcggtagag
      601 caccgcgatc ttcatcgcga gctcgagccg gtgactctcg ctgccggcct cgacctcgcg
      661 ctccagcacg cggatgaact cgtcgagctt gccgcgcgag cggtagaact cctcgaggtc
      721 ggtccaccgc gcctcggtga cgtacagctt cttgagcgcg tcctgggcgc gccggttgtt
      781 ctcgtcgatc cgcagcaggc gctgccaggc ctcgatcgcc ttgctgctgt tctcgacctt
      841 ctcggtgtag agcaggccga gccgctgcag cgcgtcggcg cgcagcttgt cgtcggacgc
      901 ggcgtcagcc tgccggctgc agacctcggc gagcttgtcc cactccttgt tgcgctcgta
      961 gagcttgtag agctcggcca gcgcctcctc gtgggtcggc tcgacctgca ggaccttctc
     1021 ccaccagtac gtgcagatct cgggcttctt gaccttggtc gcggccatcc gcgcgacctc
     1081 gatcaccttg gcggcgcgct cggcgtcggg tgcgcgctcg acctcggact ccttgagctt
     1141 gatcagcttc tcccagtcgc ggcgcttctc gtagaccgcg agcaggtgat cgaccgcctg
     1201 cttgttgtcg gggtcgagct ccagcacgcg ctcgaacgcc ttgatcgcct cggcctggtt
     1261 ggagaaccgc tcgaggtaga ggttggcgac ctgcagggtg agcgcgatct tgtcgacgag
     1321 ctcgacccgc tcggccttct cgagcaggac cttgaccagg tccggccagc gcttcttggc
     1381 ctcgtacagc gcgctcagcc ggtcgtagac ctcgagccgc gacggatcct gggcgagcgc
     1441 cgacgacagc gcggtcatca cctggttgtc gttgttgagc ttgccgtagg cgtcggcgag
     1501 ctcgagcagg atcgcggcct tgtcgggccc ggcgggcgcg accttggcct cctcg
//
LOCUS       DASVOR010002285         6014 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_170684,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002285 DASVOR010000000
VERSION     DASVOR010002285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170684"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="VF516_42255"
     CDS             complement(<1..325)
                     /locus_tag="VF516_42255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114448.1"
                     /translation="MRLGAWRVERTLGHDLSGTYYAGRNDAGERATLYLLSGELAATR
                     REPLARLIALHRELAHPGLVRFSGLDHDGSDRFLIGDAVDDALASLRSGRRPDPEQAR
                     AFGAAL"
     gene            complement(616..1404)
                     /locus_tag="VF516_42260"
     CDS             complement(616..1404)
                     /locus_tag="VF516_42260"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114449.1"
                     /translation="MATGRKVAVKLLHSDMTYDNEVVARLRREVMLVCKLRDAHTATI
                     YDVDQTPWGTPYIAMELLEGESLNHTLCLGPLEWKRVFKILVEACSSLAEAHAQGIVH
                     RNLKPENIYLEWRSGNLEFTKILDYGIANLFGRGQRTRGSLPSTTNVLPLVQVLEYAS
                     PEQLMGRALDGRSDIYALGVLAYAMITWRLPFPEARGPQGLISAQLKQTPSAPSLVCP
                     GARLPRAADRAILRCLEKDRHNRYPDVTQLAAELQALIADPRGA"
     gene            1285..>6014
                     /locus_tag="VF516_42265"
     CDS             1285..>6014
                     /locus_tag="VF516_42265"
                     /inference="COORDINATES: protein motif:HMM:NF017539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8114450.1"
                     /translation="MRVAQLAHQHDLAAKPRDYLVVVGHVGVQELDRDLAAGRQLHGA
                     VHRADPPRADLRLDLEPIAEHLAEVLFLGLGARTASRALWVLRRARPRRLHPLGRITR
                     GGVARLAVAALGDRRRSDRSAAGRQPIAAHAAAPGIRLDQRVARRAVHVRGNPRGVRR
                     RWVSSPITPSWLDGTIASTTAGSCVVRGRRYRYHRHGVKMSAERLSYSIERERHERFV
                     GRDALLARLDHLLLGATGDRVVVVTGGPGMGKSALLAAWLARREQAGDAVPHHFIRRG
                     QYNWDDPAAIVDSLVSQIADRFPGGPEATGPRMHPAARLAATLARVSSTELVPHGARL
                     VVMIDSLDEYDPPAAVAPGDPLAEFLPHALPRGVSLLCACRPRHLYVAALAARGVLVQ
                     IDLDEGDLAADNEATVRAFWDRAAPELALDATFIASAVTRAGGNLQHAALLRQHLEGL
                     APAQRRVEDIPRGLTALLDRTWERIAADAVAVEGLGILCAAREALTLDELSTVAGWSD
                     VARRRSFLRGARELLVANDRGDGHDEYRLHHELIRAHISHAIGATALHEHHRALAHRL
                     ATWPLPAEPAARRYALRHALVHHAEAGDSTELWRVAADARFMATRCRELSVHDVEADV
                     LRAAERCRARGDDAMWRRLGELARALARESHWLRAAPDATAALMWNRLRRSGWSAAVA
                     DQELGVAGIQTFLRVRHVATRESPALERDLVGHAASVLACALTANGVRAASASADGTM
                     KVWDTATGYVLATLEGHTSWVMACALTADGRRMISGSADGTVNLWDTAAWRILATLEA
                     HTGPVRACAVTADGRRIVSGSADGTVNIWDAVDESARARTSEREAAEDPRDRIRDDGP
                     REYALRVTLEGHVAPVLACAVTPDGARVVSASEDRTLKVWDPMTGHALATLEGHAGAV
                     RSCAVTADGTRIVSASVDRTLKIWDPAAGRVLTTLNGHSAAVRACAMTADGERVVSAS
                     EDRTLKVWDPTTGHALATLQGHSAAVRACAVTADGRRTISASADGTLKVWDTADAGLL
                     AAAHGLEGHAAAVRALAVTPDGRRILSASLDGTLKIWDPATGRSLATLEGHSAAVRGC
                     AVTADGRVISASMDGTLKIWDPVTSRSLATLEGHSAAVRACAVTADGRVISASEDCTL
                     KIWDAATARSVVTLRGHSGPVVACAMVAGGKQVVSASTDGTLKVWDTGTGHVFATLGS
                     HSSQVLALALTGDGRVVSASADGTLKIWALDGRLIATLRGHSGWVLACAVTMDGRRVV
                     SASDDRTLRVWEVASGRALATLEGHAGQVHTCVLTADSRHAISVSEDWTLKVWDLETY
                     RCSFTHRGDTEFLTVAATTSGIAAGDARGAMWFLDLPPVAPSTTQRRAGSDPAGSAVR
                     SEDYSAQTVDVGILTIRDDEFRAVLDAFPNKAGAGVYQGARRQYILRYAEIATGEHYT
                     VAILRHIEQGTGEAQHAARDLIDDLRPRLVLVVGIAGGLPSDDVTLGDIVLGTRIHDY
                     TVQMIAVGHETTYAATGGPIDHAIAAAVAMLPGREDELGDWTSGLPLPPAVTWAKRGQ
                     LYGPPEWQRELRAKLEHHHGKRAQ"
BASE COUNT          876 a         2048 c         2173 g          917 t
ORIGIN      
        1 ccagcgcggc gccgaacgcg cgggcctgct cgggatccgg gcgccgcccg ctgcgcagcg
       61 acgccagcgc gtcgtccacg gcatcgccga tcaggaaccg gtcgctgccg tcgtgatcga
      121 ggccggagaa ccggaccagg ccggggtgcg ccagctcgcg atgcagcgcg atcagccggg
      181 ccagcggctc gcggcgggtc gctgcgagtt ctcccgacaa gaggtacagc gtcgcccgct
      241 cgcccgcgtc gttgcggccc gcatagtagg tgccggacag atcgtggccg agcgtgcgct
      301 cgacacgcca ggcacccaga cgcatggcca ttgcattcat agctgtgccg ctcggcacgc
      361 ctccctccag gccttagccg agctagtctc tgatggtagg cgtccgggtc cgtcgtcgca
      421 agggcgaggc gcggagcatc tggtggagcc gccggtgcgg cgggccgtgc ccgccacggc
      481 tcgccggccc ggcgaaccag ctcggttccc tgcgtgccga cgtacagcgc tccgtcgatc
      541 gccttcgacg cggcgaacgc ctccagatcg ccgccatcgc ggttcgccgc caggcggcag
      601 cgtgccagcg ctcgatcagg ctcctctcgg atccgcgatc agcgcctgca gctctgccgc
      661 cagctgggtg acatccgggt agcgattgtg ccgatccttt tcgaggcacc tcaggatcgc
      721 gcgatcggcg gcgcgaggca gcctcgcgcc cgggcagacc agcgaggggg cggagggggt
      781 ctgcttgagc tgcgccgaga tcaagccctg cggccccctg gcctcgggga acggcaatcg
      841 ccaggtgatc atcgcgtagg ccaggacgcc gagcgcgtag atatcgctgc ggccgtcgag
      901 cgccctaccc atcagctgct cgggagatgc gtactcgagg acctgcacca acggtagaac
      961 gtttgtcgtg ctcggcagcg agccccgagt gcgctgtcct cttccaaaga ggttggcgat
     1021 gccgtagtcg aggatcttgg tgaactcgag gttccccgag cgccactcga ggtagatgtt
     1081 ctcgggcttg agattgcgat ggacgatgcc ctgcgcgtgg gcctcggcga gcgagctgca
     1141 cgcctcgacg aggatcttga acacccgctt ccactccagc ggccccaagc agagcgtgtg
     1201 gttcagactc tcgccctcga ggagctccat cgcgatgtac ggcgtgcccc acggcgtctg
     1261 atcgacgtcg tagatcgtgg ccgtgtgcgc gtcgcgcagc ttgcacacca acatgacctc
     1321 gcggcgaagc cgcgcgacta cctcgttgtc gtaggtcatg tcggagtgca agagcttgac
     1381 cgcgaccttg cggccggtcg ccagttgcac ggcgcggtac accgcgccga tccgccccgc
     1441 gccgatcttc gactcgatct tgaaccgatc gctgagcacc tggccgaggt actcttcctc
     1501 ggtctcgggg cgcggactgc gagccgtgcc ctttgggttc tgcgccgggc tcgtccccgc
     1561 cggctgcatc cgctcggccg gatcactcgg ggcggcgtgg cccggctcgc cgtggcagcg
     1621 ctcggtgatc ggcggcgttc cgaccgttcc gcggctggga ggcagccgat agccgcacac
     1681 gccgcagcac cgggcattcg gctcgaccag cgagtggcac gacgagcagt tcatgtgcgg
     1741 gggaaccctc gcggagtgcg tcgtcgttgg gtctcgtcgc cgatcacgcc gtcctggctg
     1801 gacggtacca tagcatcgac cactgcagga tcgtgcgtgg tgcgcggccg gcggtaccgc
     1861 taccatcgcc acggggtgaa gatgtccgcc gagcggctca gctacagcat cgagcgcgag
     1921 cgccacgagc gcttcgtcgg tcgcgacgcg ctgctcgccc ggctggatca tctcctcctc
     1981 ggcgcgaccg gcgatcgcgt cgtcgtcgtg accggtggtc cagggatggg caagagcgcg
     2041 ctgctggcgg cgtggcttgc gcggcgcgag caagcgggcg acgcggtgcc gcatcacttc
     2101 atccgcaggg gccagtacaa ctgggacgat ccagcggcga tcgtcgactc gctcgtgtcg
     2161 cagatcgcgg atcggtttcc cggcggtccc gaggcgaccg gaccccggat gcacccggcg
     2221 gcgcggctcg cagcgacgct ggcacgcgtg tcgtcgaccg agctggtgcc gcacggtgcg
     2281 cgcctggtcg tgatgatcga cagcctcgac gagtacgatc cgcccgcggc cgtggccccc
     2341 ggcgacccgc ttgcggaatt cctgccccac gccctgccgc gcggcgtgag cctgctgtgt
     2401 gcctgccgcc cacgacacct ctatgtcgcg gcactcgctg cacgaggcgt gctcgtccag
     2461 atcgatctcg acgagggtga cctcgcggca gacaacgagg ccacggtgcg cgcattctgg
     2521 gaccgtgcgg ccccggagct cgcgctcgac gcaacgttca ttgccagcgc ggtcacgcgc
     2581 gccggcggca acctgcagca cgccgcattg ctgagacagc atctcgaagg gctggccccg
     2641 gcgcagcggc gcgtcgagga catcccgcgc ggcttgacgg cgctgctcga tcgaacgtgg
     2701 gaacggatcg cggccgatgc ggtcgcggtc gagggcctcg gcatcctgtg cgccgcgcgc
     2761 gaggcgctga cgctcgacga gctgagcacg gtcgcagggt ggtccgacgt cgcacggcgc
     2821 cgcagctttc tgcgcggcgc acgcgagttg ctcgtggcga atgaccgggg cgatggccac
     2881 gacgagtacc ggctgcacca cgagctgatc cgcgcgcata tcagccatgc gatcggtgca
     2941 acggcgctgc acgagcatca ccgcgcgctg gctcaccggc ttgcgacctg gccgctcccg
     3001 gcagaacccg cggcacggcg ctacgcgctc cgccatgcgc tggtccatca cgccgaggcg
     3061 ggcgattcga ccgagctgtg gcgcgttgcc gccgacgccc ggttcatggc gaccaggtgc
     3121 cgcgagctga gcgtgcacga tgtcgaagcc gatgtgctgc gcgccgccga acgctgtcgc
     3181 gcgcgaggcg acgatgcgat gtggcgccgg ctcggcgagc tggcgcgggc cctggctcgg
     3241 gagtcgcact ggttgcgtgc ggcgcccgac gcaaccgctg cgctgatgtg gaatcgactg
     3301 cggcgctcgg gctggagcgc cgcggtcgcc gatcaggagc tcggcgtggc agggatccag
     3361 acgtttctgc gcgtccggca tgttgcgacc cgagagagcc ccgcgctcga gcgcgacctc
     3421 gtcggccatg ccgcctcggt gctggcatgc gcgctgaccg cgaacggtgt gcgcgccgcg
     3481 tcggcctcgg cggacggaac catgaaggtc tgggacacgg ccaccggata cgtgctcgcc
     3541 accctcgaag gtcacacgag ctgggtcatg gcgtgcgccc tgaccgccga cggccggcgc
     3601 atgatctcgg ggtcggcgga cggaacggtg aacctctggg acaccgcggc gtggcgcatc
     3661 ctggcgaccc tcgaagccca caccggtccg gtgcgcgcat gtgcggtgac tgccgacggc
     3721 cggcgcatcg tgtcgggctc ggcggacggg accgtgaaca tctgggatgc ggtggacgag
     3781 tcggcgcgtg cgcgaacgtc ggaacgcgag gcagcggagg accctcgaga tcggatccgc
     3841 gacgacggcc ctcgagaata cgcgcttcgc gtcacgctgg aaggccacgt ggctccggtg
     3901 ctcgcatgtg cggtgacgcc ggacggtgcc cgcgtcgtgt cggcctcgga ggaccgcacg
     3961 ctgaaggtct gggacccgat gacgggacat gcgctggcca cgctcgaagg ccacgctggc
     4021 gccgtgcgct cgtgtgccgt gaccgcggac ggcacgcgca ttgtctcggc atctgtggac
     4081 cgcaccttga agatctggga tccggcggcc gggcgcgtcc tcacgacgct gaacggccat
     4141 tccgccgcgg tccgcgcttg tgccatgacc gcggatggcg agcgagtcgt gtcggcctcg
     4201 gaggaccgca cgctgaaggt ctgggatccg acgacggggc atgcacttgc gacgctgcaa
     4261 ggccactctg ccgccgtgcg cgcatgcgcg gtgacggcgg acggcaggcg cacgatctcg
     4321 gcctcggcgg atggcacgct gaaggtctgg gacacggccg acgcgggctt gctcgccgcc
     4381 gcgcatggcc tcgagggcca cgctgccgcg gtgcgggcac tcgccgtgac gcccgacggc
     4441 aggcggatcc tctccgcatc gttggacggc acactgaaga tctgggatcc ggcgaccggt
     4501 cgatcgctcg ccaccctgga aggccactcc gccgcggtgc gggggtgcgc ggtcacggcg
     4561 gatgggcgcg ttatctcggc atcgatggac ggtacgctga agatctggga tccggtcacg
     4621 agccgctcgc tcgccaccct ggagggccac tccgccgcag tgcgggcgtg tgcggtcacc
     4681 gcggatgggc gcgtcatctc ggcatcggag gattgcacct tgaagatctg ggacgcggcc
     4741 actgcgcgtt cggtggtaac gctccgcggg cactccggcc cggtcgttgc ctgcgcgatg
     4801 gtcgcgggtg gcaagcaggt ggtctcggca tcgaccgatg gaacgctgaa ggtctgggac
     4861 accggcacgg gccacgtgtt cgccacgctg gggtcccact ccagccaggt gctcgcgctc
     4921 gcactcaccg gggacggacg cgtggtctcg gcgtcggcgg atggcacgct gaagatctgg
     4981 gcgctggacg gccgcctgat cgccacgcta cgaggacact cgggctgggt gctcgcatgt
     5041 gcagtcacga tggacggccg gcgcgtggtc tcggcatcgg atgaccggac tctgcgcgtc
     5101 tgggaggtgg caagcgggcg cgcgctggcg acgctggaag gccatgccgg gcaggtacac
     5161 acgtgcgtgc tgaccgcaga tagccgccac gcgatctccg tgtccgagga ctggacgctg
     5221 aaagtctggg acctcgagac gtaccgctgt tcgttcaccc accggggcga tacggagttc
     5281 ctgaccgtcg ccgcaacgac gagcggtatc gcggcggggg atgcccgagg cgcgatgtgg
     5341 ttcttggacc tgccgccggt ggctccgagc acgacccagc gccgagctgg cagcgatcct
     5401 gccggaagtg ccgtgcgttc agaagactac tctgcgcaga ccgtggatgt tggaatcttg
     5461 acgatccgtg acgatgaatt tcgcgccgtg ctcgatgcct tcccgaacaa ggcgggagcg
     5521 ggcgtgtacc agggggctcg ccgccagtac atcttgcgct acgccgagat cgcaaccggc
     5581 gaacactaca ccgtcgcgat cctgcgccac atcgaacagg gcaccggcga ggcgcagcat
     5641 gcggcgcgcg acctcatcga cgacctccgc cccaggctcg tcctggtggt cggaatcgcg
     5701 ggcggactgc cgtcggatga cgtcacgctg ggcgacatcg tgctcgggac gcggatccac
     5761 gactacacgg tgcagatgat cgcggtcggc catgaaacca cgtatgcagc gacgggtggg
     5821 ccgatcgatc acgcgattgc ggcagccgtg gcgatgctac ccggccgcga agacgagctc
     5881 ggcgactgga cgtcggggct gccgttgccg ccggctgtga cgtgggccaa gcgaggtcag
     5941 ctgtacggtc cacccgaatg gcagcgcgag ctccgggcca agctggagca ccaccatggc
     6001 aagcgcgcgc agca
//
LOCUS       DASVOR010002286         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_403747,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002286 DASVOR010000000
VERSION     DASVOR010002286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403747"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VF516_42270"
     CDS             <1..303
                     /locus_tag="VF516_42270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114451.1"
                     /translation="LALGADPGMLVAPLGPEIAAQKGVQLRDAVEFEPGVSFRETKEK
                     WNELFERRYLTWLIRRAEGNISKAARDADMDRKYLHKLLRKYGITATEAAGDDDEN"
     gene            complement(542..799)
                     /gene="rpsT"
                     /locus_tag="VF516_42275"
     CDS             complement(542..799)
                     /gene="rpsT"
                     /locus_tag="VF516_42275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEX8114452.1"
                     /translation="MANIASAEKQNRKMIKHRARNRAAMATLRTAVKKARAAVDDKSN
                     EATALVREAVSIIDSAVTKGILKRRTASRYVSRLAMRKPAA"
     gene            complement(1042..>1521)
                     /locus_tag="VF516_42280"
     CDS             complement(1042..>1521)
                     /locus_tag="VF516_42280"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEX8114453.1"
                     /translation="TGVATSAQVLAQWDEIKRAEKAAAGARDRILAGVKPGPALSRAQ
                     KIGTKAAKVGFEWPDWQGSFAKVEEEVREVAEAIRGGDTAAIHAELGDLLFAVVNVAR
                     KVGVDAENALIDATSRFQRRFEAVEDALADRGKTPASSTLEEMDALWDDAKRRERQR"
BASE COUNT          221 a          555 c          492 g          253 t
ORIGIN      
        1 ctcgcgctcg gcgctgatcc gggcatgctc gtcgcgccgc tcggtcccga gatcgccgcg
       61 caaaagggcg tccagctccg cgacgccgtc gagttcgagc ccggggtgtc gttccgcgag
      121 accaaagaga agtggaacga gctgttcgag cgccgctacc tgacctggct gatccggcgc
      181 gccgagggca acatctccaa ggcggcgcgc gacgccgaca tggatcgcaa gtacctccac
      241 aagctgctgc gcaagtacgg catcaccgcg accgaagccg cgggcgacga cgacgagaac
      301 tagggaagcg acggcgctcc ccggcacgcc gttcgatccc tggtaccgag tcgacgagct
      361 gaccgtcgcg tggacgggac actgaatctc gcggcgagac cggaacaccg gatcagcggt
      421 cacgcgcgcg gcgccgggcg aacgacgcgc ggaacatcgc ggcggcggct ctggcgcgag
      481 cccccaaggg ctctagcgcc ggctcccgcg ctgtggtcgg gccggcgagc ctgtcttcgc
      541 cctaggccgc cggctttcgc atcgccaggc gagacacgta gcgcgacgcg gtgcggcgct
      601 tgaggatgcc cttggtgacc gcgctgtcga tgatcgacac ggcctcgcgc accagggcgg
      661 tggcctcgtt cgacttgtcg tcgaccgcgg cccgcgcctt cttgacggcg gtccgtaacg
      721 tggccatcgc cgcgcggttg cgcgcgcgat gcttgatcat cttgcggttt tgcttctcgg
      781 ccgacgcgat attggccacg acgaactctc ctggggaggc gagtggttaa cccgcccagc
      841 tcccgtagtc aagccggtcg cgacgatgag cgtcgggggc cggggggcgg tccgggctgc
      901 ccgccatttt gctaacaacc ccgcgggagt acccacggcg gttgtgaata acttgcggac
      961 cgcttggtga tggcggggtt gatctcatcg agatctctct cactcgatct cgcctgctca
     1021 aaaaagcatc actttcgtca ctcaccgctg gcgctcgcgt cgtttggcgt cgtcccacag
     1081 cgcgtccatc tcctccagcg tcgacgacgc gggtgtcttg ccacggtccg ccagcgcgtc
     1141 ctcgaccgcc tcgaaccgcc gctggaaccg gctggtcgcg tcgatcagcg cgttctctgc
     1201 atcgaccccg accttgcgag ccacgttcac caccgcgaac agcagatcgc cgagctctgc
     1261 gtggatcgcc gcagtatccc cgccgcggat cgcctcggcc acctcccgga cctcctcctc
     1321 gacttttgcg aacgacccct gccagtccgg ccactcgaac ccgaccttgg cggccttggt
     1381 cccgatcttc tgggcccggc tcagcgccgg accgggcttg accccggcga ggatgcggtc
     1441 gcgggccccc gccgccgcct tctcggcgcg cttgatctcg tcccactgcg ccagcacctg
     1501 cgcgctggtc gcaactcccg t
//
LOCUS       DASVOR010002287         3154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113371,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002287 DASVOR010000000
VERSION     DASVOR010002287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113371"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VF516_42285"
     CDS             complement(<1..858)
                     /locus_tag="VF516_42285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8114454.1"
                     /translation="MRRDEILQIWEAAVRAVPQARKLDRPTLIDHVPDLLEEIAHAAE
                     ELEHGRAPDLRPGEAERHTLARLDQGFDLLEVVGELRALRRCIVKLFAREHQGVIGAD
                     ELEVLEDAIDAAVAGSIEQYTLVRERTLQGATVVQHALRDQARRAEAQLAEQERQFSA
                     LADHVPQLVWMSDPAGGCYWCNRRWCEYTGTTLEEMRGRGWEKVVRPDHAPRVVEHWR
                     RAVEHGLPWEDTFPVRGRDGHYRWFLSRAHPIRDASGRIERWVGTNTDVTAQRFLDDA
                     TRILSGTLNM"
     gene            complement(1032..1766)
                     /locus_tag="VF516_42290"
     CDS             complement(1032..1766)
                     /locus_tag="VF516_42290"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEX8114455.1"
                     /translation="MLRSVRIAKRAIDVAGGLLGLALTAPLMPLIAAAIYIDSPGPVL
                     FRQRRAGSLRAIKTENGKKQFQFDEFEMHKFRTMVPNAEAKTGVVVAGAGDPRITRVG
                     KFLRKSRLDELPQFWDVLRGKMSLVGPRPERPELLQDLSYAIPLFEERMRDVKPGITG
                     LAQISLGYTGSIPEGSEIGKLAATLQNPWGLPDAAGSLADDMRIKLLYDLAYTLSLEQ
                     FGTFLRTELGIIFKTPLVMIRMTQGR"
     gene            1962..2450
                     /locus_tag="VF516_42295"
     CDS             1962..2450
                     /locus_tag="VF516_42295"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8114456.1"
                     /translation="MAAPIRLSVPGTLRYRPVAVRAVAEAARLVSCPSPVDRRDPDPL
                     ALDIRHPFDTAVVSAFMEIFNNIAIHAYQRRGGGTIDIEITLTADDILIEVKDSGRAF
                     DLDNVVPLPTDLDEDALPEGGMGIHIAKTMLDDMTYEPGPPNQWRLYKRLPPAQVAVG
                     RS"
     gene            2452..2754
                     /locus_tag="VF516_42300"
     CDS             2452..2754
                     /locus_tag="VF516_42300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEX8114457.1"
                     /translation="MAPFEVNRTNDDTTLRVRGSLDINSAPALAEEIDRIVALRPPKV
                     IVDLSMLDLIDSSGVAALVKLYKEVRKTGGAMKIAGARDQPLAIFKLLRMDKVFDL"
     gene            2838..>3154
                     /locus_tag="VF516_42305"
     CDS             2838..>3154
                     /locus_tag="VF516_42305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114458.1"
                     /translation="MRVLAITKIFPNQAEPLSAPFNRQQFAALARRCELEVAATIPWF
                     PGAGLISRWSSAGRLARVPRREQIAGIAVTHPRTLFVPRLAHGAWGPLYAASIAPALV
                     RYRG"
BASE COUNT          483 a         1098 c         1076 g          497 t
ORIGIN      
        1 catgttcagg gtgccgctga ggatgcgggt cgcgtcatcg aggaagcgct gcgcggtgac
       61 gtcggtgttg gtcccgaccc agcgctcgat cctgccggag gcgtcgcgga tcgggtgcgc
      121 gcgcgacagg aaccagcggt aatggccatc cctgccccgc acggggaacg tatcctccca
      181 gggcaggccg tgctcgaccg cacggcgcca gtgctcgacg acccgtgggg cgtgatcggg
      241 gcggacgacc ttctcccatc cccgaccccg catctcctcg agcgtggttc cggtgtactc
      301 gcaccagcgc cggttgcacc agtagcatcc ccccgccggg tcgctcatcc acacgagctg
      361 cggaacgtgg tcggcgagtg cgctgaactg acgctcctgc tccgcgagtt gcgcctccgc
      421 gcgccgcgcc tggtcgcgga gcgcgtgctg gacgacggtg gcgccctgca gcgtgcgctc
      481 gcgaaccagg gtgtactgct cgatcgaacc ggcgaccgcc gcatcgatcg cgtcctcgag
      541 gacttcgagc tcgtcggcgc cgatcactcc ctggtgctcc ctggcaaaca gcttgacgat
      601 gcaccgccgc agcgcgcgaa gctccccgac cacctcgagc agatcgaacc cctgatcgag
      661 ccgggcgagg gtatgtcgct cggcttctcc cggtcgtagg tccggcgccc tgccatgctc
      721 cagctcctcg gccgcatgag cgatctcttc cagcagatct ggaacatgat cgatcaacgt
      781 cggccgatcg agcttcctcg cttgcggcac cgcgcgcacc gccgcttccc agatctgaag
      841 gatctcgtcg cggcgcatgc ggaccaggtc cgctgaacga tggggtctcg aagacgtcat
      901 ccacctcctg gagggaccgg ctcaacacac caggtatgcc tccggccggt ggcactgcat
      961 atcaggaagt ccaccgcaga aagcgtcgca tgctgcgtgc gatgcggcgg atccgcccgc
     1021 gcccccggcg ctcagcggcc ctgggtcatc cggatcatga ccagcggcgt cttgaagatg
     1081 atgccgagct cggtgcgcag gaacgtgccg aactgctcga gcgacagcgt gtaggcgagg
     1141 tcgtagagca gcttgatccg catgtcgtcg gcgagcgagc ccgcggcgtc gggcagcccc
     1201 cacgggttct gcagcgtggc ggcgagcttt ccgatctcgc tgccctcggg gatcgagccg
     1261 gtgtagccga gcgagatctg ggccaggccg gtgatgccgg gcttgacgtc gcgcatccgc
     1321 tcctcgaaga gggggatggc gtacgacaga tcctgcagca gctcggggcg ctcggggcgc
     1381 gggccgacca ggctcatctt tccgcgcagc acgtcccaga actggggcag ctcgtcgagc
     1441 cgcgacttgc ggaggaactt gccgacccgg gtgatccgcg ggtcgccggc gcccgccacg
     1501 accacgccgg tcttggcctc ggcgttgggc accatcgtgc ggaacttgtg catctcgaat
     1561 tcgtcgaact ggaactgctt cttgccgttc tcggtcttga tcgcgcgcag gctgcccgcg
     1621 cggcgctggc gaaacagcac cgggccgggc gagtcgatgt agatcgccgc ggcgatgagc
     1681 ggcatcagcg gagcggtcaa cgccaggccg agcagcccgc ctgcgacatc gatggcccgc
     1741 ttggcgatcc ggacgctgcg caacacggag ctcacagtaa catggccgcg gcgcaaccgc
     1801 ggaatccgcc gccaggggcc tcgccttgac cttacgtttt gtcgatcgac aggggccgag
     1861 ccggccgatt cacccctccg cgcgtcgcgg gggctttttt cttcgagcga ggctccgcgg
     1921 actttcgcaa accccgccgg gctggctatg ctccagatca gatggccgcc ccgatccgcc
     1981 tcagcgtacc cggtacgctt cgctaccggc cggtcgcggt gcgtgcggtc gcggaggcgg
     2041 cccggctggt atcgtgcccg tcgcccgtgg accgccggga ccccgatccc ctcgcgctcg
     2101 atatccgcca cccgttcgac acggcggtgg tctcggcgtt catggagatc ttcaacaata
     2161 tcgcgatcca cgcataccag cgccgcggcg gcgggacgat cgacatcgag atcacgctga
     2221 ccgccgacga catcctgatc gaggtcaagg acagcggccg ggcgttcgac ctcgacaacg
     2281 tcgtgccgct gccgaccgat ctggacgagg acgcactacc ggagggcggg atgggcatcc
     2341 atatcgcgaa gaccatgctc gacgacatga cgtacgagcc cgggccgccg aaccaatggc
     2401 ggttgtacaa gcgactgcca cccgcgcagg tcgccgtcgg gaggagctga catggcgccg
     2461 tttgaagtga accgtaccaa cgacgacacg acactgaggg tgcgcgggtc gctcgacatc
     2521 aacagcgcgc cggcgctggc cgaggagatc gaccggatcg tcgcgttgcg tccacccaag
     2581 gtgatcgtcg atctctcgat gctcgacctg atcgacagct ccggggtggc cgccctggtc
     2641 aagctgtaca aggaggtgcg caagaccggc ggcgccatga agatcgccgg cgcgcgcgac
     2701 cagccgctcg cgatcttcaa gctgctgcgc atggacaagg tgttcgacct ctagtgtgcg
     2761 ccaccgcgcg gtgtccgtgt gcggaagcgc ccggtcacgg ccggtgccgg tagggagccg
     2821 acagggagcg tgagccgatg cgtgtcctcg cgatcacgaa gatcttcccc aaccaggccg
     2881 agccgctgtc cgcgccgttc aaccgccagc agttcgcggc gctggcacgg cgctgcgagc
     2941 tcgaggtcgc cgcgacgatc ccgtggtttc cgggcgcggg cctgatctcg cggtggtcga
     3001 gcgccggccg gctggcgcgg gtgccgcgcc gcgagcagat cgccgggatc gccgtgaccc
     3061 acccgcgcac gctgttcgtg ccgcgactgg cgcacggcgc gtggggcccg ctgtacgcgg
     3121 cgtcgatcgc cccggcgctg gtgcgctacc gcgg
//
LOCUS       DASVOR010002288         4086 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_403787,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002288 DASVOR010000000
VERSION     DASVOR010002288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403787"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="VF516_42310"
     CDS             <1..1008
                     /locus_tag="VF516_42310"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114459.1"
                     /translation="RAPEIAAAGAALAVLDACGSLAAVAARRGYCRPTVDDGLVLDIT
                     DGRHPVVEAMLPAGSFVPNDARLDPAAEQLVLITGPNMAGKSTYMRQVAQIVLLAQIG
                     GFVPARAAQIGVCDRLFTRVGAADNLSRGDSTFMVEMRETAAILARATRRSLVVLDEV
                     GRGTSTFDGVSIAWAVAEFLHDAIGARTLFATHYHELVALADSRPRVRNVSVAVREHK
                     GEIVFLRRVIPGGASKSYGIDVARLAGLPRSVIARARQIMGQLESGSQALGLGSSRQL
                     SLLPPGLPPGLPPGVPPGVPPAVPGAPPSPVLARLAAIDIDRLTPLEALQLLAELRSL
                     V"
     gene            1245..3464
                     /locus_tag="VF516_42315"
     CDS             1245..3464
                     /locus_tag="VF516_42315"
                     /inference="COORDINATES: protein motif:HMM:NF037276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114460.1"
                     /translation="MKQHIQSIAIGAALFTLGALGCGGSDSGPLSNVDSLIILQRPKR
                     NDVGNVFEYTSYKPGARLVQLKPPTANGTVTPICCGQDADFKNVDIADYDLSFDAKTI
                     VFSARRGSDPSFGLFLLELSDGKITQVATDPTRDFVSPIFLPGNKILFTTNAVVEAGA
                     RQHEDEYERATTSQLGRVNVDGTNLELGPRNLSHRRTPSLASDGRVMFTQWDHLGETN
                     EANLMFVNQDMQELREGFGKEGTGAANSYLRAREISPGRFTAIATARDRTIQAGAIID
                     IRLGDVVSQDGVVSAPNNQSEAHATFRALTPDVPMGNAPSAETIGRYYDAFPLNAKDK
                     PDLLVSWADGPVESEVLAMAGLSANFGIYLYDSEHQQRRPILDDPDMWDVLARPLATR
                     SAPASVSSAQNPRPGGSVLIGSLDVYDSSLHQFNRPAPGQPGEIYGVRVMEGFSSEEG
                     FPTMFGTTMFEGHANLGVAPISADNKSWSAMIPANIPVHLQAVDVFGMSLFNEPVWIS
                     GRAGEARMCGGCHEDRTKTTNVTPGLLDTFANGATQLMPSVPRSARLNAAPANPTDIV
                     GVAWDKHLQPIFDQSCVTGCHDANNSAGITGYSIVDAMGNKIADWHLDLSGTKVPAAL
                     GVVAGGGAFTASYFSMAGPDEEAIERNGLKIVPPDATTKYMIPLDAHSSKVIQKLNPT
                     QLFPAINAAKRAFSTPAHLVALGKTDLTPMQFYLLILAADMGVNFYARENNPMMNNP"
     gene            3493..>4086
                     /locus_tag="VF516_42320"
     CDS             3493..>4086
                     /locus_tag="VF516_42320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8114461.1"
                     /translation="MKNIIKLAAVGLLALAPSAAFAGKGGSAAAIQAAVASGSSDAIA
                     AELERTESLICEECVGLVTALTEDSRYSVREVAAWWFGRRPALRDMLVDGFVADLPHG
                     DTIKVRNAADFLGRTRTLSAIAPLRAAIQRGDLGVEARLAVVRAVGYMGHLSGNDVLV
                     TGMADPDASVRGAAVIAWRDMLGQPTAAPVVPLLGDLD"
BASE COUNT          599 a         1550 c         1361 g          576 t
ORIGIN      
        1 cgcgcccccg agatcgccgc cgccggcgcc gcgctcgccg tgctcgacgc gtgcggctcg
       61 ctcgccgcgg tcgccgcccg ccgcggctac tgccgcccga ccgtcgacga cggcctggtg
      121 ctcgacatca ccgacggccg ccacccggtg gtcgaggcca tgctgccggc cggcagcttc
      181 gtgcccaacg acgcccggct cgatccggcc gccgagcagc tcgtgctgat caccggcccc
      241 aacatggccg gcaagtcgac ctacatgcgc caggtcgcgc agatcgtcct gctcgcccag
      301 atcggcggct tcgttccggc ccgggccgcg cagatcggcg tgtgcgatcg gctgttcacc
      361 cgggtcggcg ccgccgacaa cctgtcgcgc ggcgactcga cgttcatggt cgagatgcgc
      421 gagaccgccg cgatcctcgc ccgggcgacc cggcgctcgc tggtggtgct cgacgaggtc
      481 ggccgcggca cctcgacgtt cgacggcgtg tcgatcgcct gggcggtggc cgagttcctg
      541 cacgacgcga tcggcgcccg cacgctgttc gccacccact accacgagct ggtcgcgctt
      601 gccgacagcc gcccgcgggt ccgcaacgtc tcggtcgcgg tccgcgagca caagggcgag
      661 atcgtgttcc tgcgccgggt catccccggc ggcgccagca agagctacgg catcgacgtt
      721 gcccggctcg ccggcctgcc gcgctcggtg atcgcccggg cccgccagat catgggccag
      781 ctcgagagcg gctcgcaggc gctcggtctg ggcagctcgc gccagctatc actgctgccc
      841 cctgggctgc cccctgggct gccccctggg gtcccccctg gggtcccccc tgcggtgccc
      901 ggcgcgccgc cgtcgccggt gctcgcccgg ctcgccgcga tcgacatcga ccggctcacg
      961 ccgctcgagg cgctgcagct gctcgccgag ctcaggtcgc tcgtctgatc gcgcgtcccg
     1021 gtcacgcgtc ccggtcacgc gtcccggtca cgcgccggca tgtaggtcga ggtgaccttg
     1081 cggctacacg gcgtgtggct ttccaccccc agcgccgcgg tcataggggg cggcagccct
     1141 gcgagcaggg ctgccgccct cgcgtcaaca cgttggaatc gttgcggttg cgagttggcg
     1201 cggcgcttgt acggaggccc atcactcgtt cgggaggcat gtccgtgaag caacacatcc
     1261 agagcatcgc gatcggcgcc gcgttgttca ccctcggggc gctcggctgc ggcggcagcg
     1321 actcgggtcc gctgagcaac gtcgactcgc tcatcatcct gcagcgcccc aagcgcaacg
     1381 acgtcggcaa cgtgttcgag tacacgtcgt acaagcccgg cgcgcggctg gtccagctca
     1441 agccgccgac cgcgaacggc acggtgaccc cgatctgctg cggccaggac gccgacttca
     1501 aaaacgtcga catcgccgac tacgacctgt cgttcgacgc caagacgatc gtgttctcgg
     1561 cgcggcgcgg cagcgatccg agcttcgggc tgttcctcct cgagctgtcc gacggcaaga
     1621 tcacccaggt cgcgaccgat ccgacgcgcg acttcgtgtc gccgatcttc ctgccgggca
     1681 acaagatcct gttcacgacc aacgccgtcg tcgaagccgg cgcccggcag cacgaggacg
     1741 agtacgagcg cgcgaccacc agccagctcg gccgggtcaa cgtcgacggc accaacctcg
     1801 agctcggccc gcgcaacctg tcgcaccgca ggacgcccag cctggcgtcc gacggccgcg
     1861 tgatgttcac ccagtgggac cacctcgggg agacgaacga ggccaacctg atgttcgtca
     1921 accaggacat gcaggagctg cgcgaggggt tcggcaagga gggcaccggc gcggcgaact
     1981 cgtacctccg ggcgcgcgag atcagcccgg gccggttcac cgcgatcgcg accgcgcgcg
     2041 accgcaccat ccaggccggc gcgatcatcg acatccggct cggcgacgtc gtgagccagg
     2101 acggcgtggt cagcgcgccg aacaaccagt ccgaggccca cgcgacgttc cgcgcgctga
     2161 cccccgacgt gccgatgggc aacgccccgt cggccgagac catcggccgc tactacgacg
     2221 cgttcccgct caacgccaag gacaagcccg atctgctggt gtcgtgggcc gacgggccgg
     2281 tcgagtccga ggtgctggcg atggccgggc tgtcggcgaa cttcgggatc tacctgtacg
     2341 acagcgagca ccagcagcgc cggccgatcc tcgacgatcc ggacatgtgg gacgtcctcg
     2401 cgcgcccgct cgcgactcgc tcggcgcccg cctcggtcag ctcggcgcag aacccgcggc
     2461 ccggcgggag cgtgctgatc ggctcgctcg acgtctacga ctcgtcgctg caccagttca
     2521 atcgcccggc ccccgggcag cccggcgaga tctacggcgt gcgcgtgatg gagggcttct
     2581 cgtcagagga gggcttcccg acgatgttcg gcaccacgat gttcgagggc cacgccaacc
     2641 tcggcgtcgc cccgatctcg gccgacaaca agtcgtggtc ggcgatgatc cccgcgaaca
     2701 tcccggtcca cctgcaggcg gtcgacgtgt tcggcatgtc gctgttcaac gagccggtgt
     2761 ggatctcggg ccgcgccggc gaggcccgga tgtgcggcgg ctgccatgag gaccgcacca
     2821 agaccaccaa cgtcacgccc ggcctgctcg acacgttcgc gaacggcgcg acccagctga
     2881 tgcccagcgt tccgcgcagc gcccggctca acgccgcgcc ggccaacccg accgacatcg
     2941 tcggcgtcgc ctgggacaag cacctccagc cgatcttcga ccagagctgc gtcaccggct
     3001 gccacgacgc caacaacagc gcgggcatca ccggctactc gatcgtggac gcgatgggca
     3061 acaagatcgc cgactggcac ctcgacctca gcggcaccaa ggtgccggcc gcgctcgggg
     3121 tggtcgccgg cggcggcgcg ttcaccgcct cgtacttcag catggccggc cccgacgagg
     3181 aggcgatcga gcgcaacggc ctgaagatcg tccccccgga cgccacgacc aagtacatga
     3241 ttccgctcga tgcgcacagc tcgaaggtga tccagaagct caacccgacg cagctgttcc
     3301 cggccatcaa tgccgccaag cgcgcgtttt cgaccccggc tcacctcgtg gcgctgggca
     3361 agaccgacct gacgccgatg cagttctacc tgctgatcct cgccgcggac atgggcgtga
     3421 acttctacgc ccgcgagaac aacccgatga tgaacaaccc ctgagcgccc ccgcgcaacc
     3481 cggagaatga ccatgaagaa catcatcaag ctcgccgcgg tcggcctcct cgcgctcgcc
     3541 ccgtcggcgg cgttcgccgg caagggcggc agcgcggccg cgatccaggc cgccgtggcc
     3601 tcgggctcgt ccgacgcgat cgccgccgag ctcgagcgca ccgagtcgct gatctgcgag
     3661 gagtgcgtcg ggctcgtcac cgccctgacc gaggactcgc gctactccgt ccgcgaggtc
     3721 gccgcgtggt ggttcggccg ccgcccggcg ctccgcgaca tgctggtcga cgggttcgtc
     3781 gccgacctgc cgcacggtga cacgatcaag gtccgcaacg cggccgactt cctcggccgg
     3841 acccgcacgc tgtcggcgat cgcgccgctg cgcgcggcga tccagcgcgg cgacctcggc
     3901 gtcgaggccc ggctcgccgt ggtccgcgcc gtcgggtaca tggggcacct cagcggcaac
     3961 gacgtgctgg tcaccgggat ggccgatccc gacgccagcg tgcgcggcgc ggcggtgatc
     4021 gcgtggcgcg acatgctcgg ccagccgacc gcggcgccgg tggtgccgct gctcggcgat
     4081 ctcgac
//
LOCUS       DASVOR010002289         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_141583,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002289 DASVOR010000000
VERSION     DASVOR010002289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141583"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1477
                     /locus_tag="VF516_42325"
     CDS             <1..1477
                     /locus_tag="VF516_42325"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8114462.1"
                     /translation="EEAYVATGEVLDTVPADPPSWARVWAAATRARSAVNLDRHTEGK
                     GWAEEALVGARALGLPDAEADALTSLALVAEIEGDSAESQTRLAEARDRAREAGDLSV
                     EGRAHYNLAASYYYAGDITSALACMDAGVARATETGLTFSVYGLEIRVLQVIARYVTG
                     DWDGSLEAAELAGDAAPDIVLARLAAAALYVEVGRGLPTAIERVAQLRGSWSKDASLA
                     LVAGGCEVDLLRWRGDLDGAADAADRAIEYVTRRWDKWYLGGIWLSALGLAALADRAD
                     GERLRGEDDAVAATVRRGDGLIERARLTAKRGRPRHGRIGPEGLAWLARAEAEWSRLS
                     GPSDPDLWREALRIFGYGYPYEEARCRWRLAEALLAQDRRDEAAAEVGAAYEIAVRLG
                     ATPLKEALEALARRGRLEAGLPVPVRVDQTLTARERDVLALVARGRTNRQIGRTLFIS
                     EKTASVHVSNILSKLGASGRTEAVAIASRRGLVPVESSQAG"
BASE COUNT          189 a          568 c          638 g          179 t
ORIGIN      
        1 cgaggaggca tacgtggcga ccggcgaggt gctcgacacg gtgccggccg acccgccgtc
       61 gtgggccagg gtgtgggccg cggcgacccg ggcccgctcg gcggtcaacc tcgaccggca
      121 caccgagggg aagggctggg ccgaggaggc gctggtcggc gcccgcgcac tcggactgcc
      181 cgacgccgag gccgacgcgc tgaccagcct cgctctggtg gccgagatcg aaggcgactc
      241 ggccgagtcg cagacccggc tggcggaggc gcgcgaccgc gcccgggagg ccggcgacct
      301 gtcggtcgag gggcgcgccc actacaacct cgccgccagc tactactacg ccggcgacat
      361 caccagcgcg ctggcctgca tggacgccgg ggtcgcccgg gcgaccgaga ccggcctgac
      421 gttcagcgtg tacgggctgg agatccgggt gctgcaggtc atcgcccggt acgtgaccgg
      481 cgactgggac ggcagcctgg aggcggccga gctcgccggc gacgccgcac ccgacatcgt
      541 gctggcccgg cttgccgccg ccgcgctcta cgtcgaggtc ggtcgggggc tgccgaccgc
      601 gatcgagcgg gtcgcgcagc tgcgcggctc ctggagcaag gacgccagcc tcgcgctggt
      661 cgccggtggc tgcgaggtcg acctgctgcg ctggcgcggc gacctcgacg gggccgcgga
      721 cgccgccgac cgtgcgatcg agtacgtcac gcggcgctgg gacaagtggt atctcggcgg
      781 catctggctg tccgcgctcg gcctcgccgc gctggccgat cgggccgacg gcgagcggct
      841 gcgcggcgag gacgacgcgg tcgcggcgac cgtacggcgg ggcgacgggc tgatcgaacg
      901 ggcccggttg acagccaagc ggggccgccc gcggcacggg cggatcggcc cggaggggct
      961 ggcgtggctg gccagggccg aggccgagtg gtcccggctg tccggcccgt ccgaccccga
     1021 cctgtggcgc gaggcgctgc ggatcttcgg ctacggctac ccgtacgagg aggcgcgctg
     1081 ccggtggcgg ctggccgagg cgctgctggc ccaggaccgg cgggacgagg cggcggccga
     1141 ggtcggcgcg gcgtacgaga tcgcggtccg gctcggggcg acgccgctga aagaggcgct
     1201 ggaggcgctg gcccggcgcg ggcggctcga agccgggctg ccggtgccgg tacgggtcga
     1261 ccagacgttg accgcccggg agcgcgacgt gctggcgctg gtcgcccgcg gccgcaccaa
     1321 ccggcagatc ggccgcacgc tgttcatctc cgagaagacg gcgagcgtgc acgtgtccaa
     1381 catcctcagc aagctcggcg cctccgggcg caccgaggcg gtcgcgatcg cctcccgccg
     1441 cggcctggtc ccggtcgagt cctcccaggc cggctgacct cccggcccgc ccgctcatcg
     1501 gcggcgcgcg gcgtgggcgc cggtcgagcc ccgctgggca cgggtcgtac ggagcgtagc
     1561 cgcccgcggc tcag
//
LOCUS       DASVOR010002290         2152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_317162,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002290 DASVOR010000000
VERSION     DASVOR010002290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317162"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="VF516_42330"
     CDS             <1..1496
                     /locus_tag="VF516_42330"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8114463.1"
                     /translation="NATHDGIFIHDDTGRVLDVNDRGCVMFGLDTSEARHAPLVELSP
                     GEPPYSQHDAFDRLRSAISSGPQVFDWQSRRADGTAFWSEIALRSFRIDGSVRVIASV
                     RDVTDRKRAGLECERLMAELQAANRAKDEFLAVLSHELRTPLAALQASIDLLLRLPTL
                     DEPRALSALRIMERNVKLQTRLVNDLLDMSRLVRGKLTLERAPMRLGEVVLSAAETCR
                     ADATRADVMLETRAEAELWVDADATRIQQMVINLIDNAIKFTPKAGRITVSVSAKEGR
                     GYVVVEDTGVGIARDRLSDIFEMFRQGEVAARRAPGLGIGLALVKSIVDLHGCRVWAE
                     SDGPGCGSRFIVELPLYDAAGVRAGRQVAASGRAVLTVLLVEDNADTRTMLAETLSQL
                     DYRVLPAETAEAALEILAREPVDAILADVGLPGMDGYEFLHQARGLPAAARVPALALT
                     GYGQDSDVRRARDAGYAGHFVKPVDIEVIDRQIRLLVSVRSSPHRPT"
     gene            complement(1497..1967)
                     /locus_tag="VF516_42335"
     CDS             complement(1497..1967)
                     /locus_tag="VF516_42335"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8114464.1"
                     /translation="MRESEYDSAIHEFHITSDGIMIASTSDSAQAVLSGRSPDVSAAP
                     AIVDDEVDIAETLQELLELAGYVVAVAFDGEDGLRKVDESRPDVIVTDIMMPGLTGLE
                     MLEQLRRNPRYKHIPVIVMSAIEREDVTVRFSAAYIKKPFGIQQLTQLLDRLPE"
BASE COUNT          328 a          743 c          734 g          347 t
ORIGIN      
        1 tcaacgccac ccacgatggg atcttcatcc acgatgacac cgggcgcgtg ctggacgtca
       61 acgatcgcgg ctgtgtcatg ttcggcctcg acacctccga ggcccgtcac gcgccgctgg
      121 tcgagctcag cccgggcgag ccgccgtact cgcagcacga cgcattcgac cgcctgcgca
      181 gcgcgatctc cagcggcccg caggtgttcg actggcagag ccggcgcgcc gacggcaccg
      241 cgttctggtc cgagatcgcc ctgcgctcgt tccggatcga cggctcggtg cgcgtcatcg
      301 catcggtgcg cgacgtcacc gatcgcaagc gcgcggggct cgagtgcgag cggctgatgg
      361 ccgagctgca ggcggccaac cgcgcgaagg acgagttcct ggcggtgctg tcgcacgagc
      421 tgcgcacccc gctggcggcg ctccaggcca gcatcgacct cctgctgcgc ttgccgacgc
      481 tcgacgagcc gcgcgcgctg tccgcgctgc ggatcatgga gcgcaacgtg aagctgcaga
      541 cccggctggt gaacgacctg ctcgacatgt cgcgcctcgt gcgcggcaag ctcacgctcg
      601 agcgcgcgcc gatgcggctc ggcgaggtcg tgctgtcggc cgccgagacc tgccgcgccg
      661 acgccacgcg cgccgacgtg atgctcgaga cccgggccga ggccgagctg tgggtcgacg
      721 ccgacgcgac gcgcatccag cagatggtca tcaacctgat cgacaacgcg atcaagttca
      781 ccccgaaggc cggacggatc accgtgtcgg tcagcgcgaa ggagggccgc ggctacgtgg
      841 tcgtcgagga caccggcgtg ggcatcgccc gcgatcgcct gtcggacatc ttcgagatgt
      901 tccgtcaggg cgaggtcgct gcgcgccggg cgcccggcct gggcatcggc ctggcgctcg
      961 tgaagtcgat cgtcgacctg cacggctgcc gggtgtgggc cgagagcgac gggccggggt
     1021 gcggcagccg gttcatcgtc gagctccctc tgtacgatgc cgcgggcgtg cgcgccgggc
     1081 gccaggtcgc tgcctcgggg cgagccgtgc tgaccgtgct cctggtcgag gacaacgcgg
     1141 acacgcgcac catgctcgcc gagaccctgt cccagctcga ctacagggtc ttgcccgcgg
     1201 agaccgccga ggcggccctc gagatcctgg cgcgcgagcc ggtggacgcg atcctcgccg
     1261 acgtcgggct ccccggcatg gacggctacg agttcctgca ccaggcccgc gggctccccg
     1321 ctgcggcgcg cgttcctgcg ctggcgttga ccggatacgg gcaggacagc gacgtccggc
     1381 gagcgcgcga cgcgggctac gcagggcact tcgtcaagcc ggtcgacatc gaggtgatcg
     1441 atcggcagat ccgcttgctc gtgagcgtac gatcctcccc ccatcgaccg acgtaatcac
     1501 tcgggcaacc gatcgagcag ctgcgtcagc tgctggatcc cgaacggctt cttgatgtat
     1561 gccgcgctga accggaccgt gacgtcctcc cgctcgatgg cgctcatgac gatcaccggg
     1621 atgtgcttgt atcggggatt ccgcctgagc tgctcgagca tctcgaggcc ggtgagcccg
     1681 ggcatcatga tatcggtcac gatcacgtcg ggccgactct cgtcgacctt ccgcaggccg
     1741 tcctcgccgt cgaatgctac cgcgaccacg tagccggcga gctccagcag ctcctggagc
     1801 gtctcggcga tgtcgacctc gtcatcgacg atggcgggtg cggcggagac gtccggcgag
     1861 cgaccgctga gcacggcctg ggcgctgtcg gacgtcgatg cgatcatgat gccgtccgag
     1921 gtgatgtgga actcgtgaat cgcgctatcg tactcgctct ccctcacctt gaggatcgat
     1981 aacaggcgat agagctgact ctgtacctcg acgtctctcg atcccgcaca agttcctgga
     2041 tcgaatcgcc gacttagcta ctcgtcgacg aggatcgtca gacagtcgtg atccaactgg
     2101 gcatggctga acgcctcgag ggtgacgcga tcgcgcggat gctggacgac ct
//
LOCUS       DASVOR010002291         1867 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_141633,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002291 DASVOR010000000
VERSION     DASVOR010002291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141633"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VF516_42340"
     CDS             complement(<1..431)
                     /locus_tag="VF516_42340"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8114465.1"
                     /translation="MMVADLTAELIAFPTQQAGPERGAGDERALCEYLAPMLRSAGAD
                     EVIVEIATRSDGGPGAYVFARWGTPHRLINVHVDTVPANAGWARDPWTPYVSEGRLYG
                     LGSADTKGAIAATLVAIEATRPRDFGVLFSGDEEAGSGVIRA"
     gene            complement(522..1091)
                     /locus_tag="VF516_42345"
     CDS             complement(522..1091)
                     /locus_tag="VF516_42345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEX8114466.1"
                     /translation="MIGPVVSGVPVVSPVMLIGQAPGVREGPAGKPFAWTAGKTMFGW
                     FARLGLEEEAFRQRVYMAAVCRCFPGKAKGGGDRVPAPDEIARCRRHLTREVELLRPR
                     LVIPVGKLAIAQLFPDAEALAQIVGQPRRGALAGVEFDVIALPHPSGASTWHRMEPGK
                     TLLAQALAEIAAHAAWRSLLDEPGAPRRN"
     gene            1280..1867
                     /locus_tag="VF516_42350"
     CDS             1280..1867
                     /locus_tag="VF516_42350"
                     /inference="COORDINATES: protein motif:HMM:NF020091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HEX8114467.1"
                     /translation="MNTPPHEPLIRRVLGGNVIELKLHRLAIEELDGGSVVKLEVLEG
                     DQPVEVEGRGVGVVEALYNALLERYAREYQSLKTIQLTGFQVAADLGTKKAQAGVDAV
                     AQVTLDVSNSEGRLFSFSDSSRSVASSTARAVLAMVQYFVNAERAFLTLYNARRDALA
                     RGREDLVARYTAEMAAVVESTSQAEVIANIRKELG"
BASE COUNT          267 a          688 c          663 g          249 t
ORIGIN      
        1 gcgcggatca cgccgctgcc cgcctcctcg tcacccgaga acagcacgcc gaagtcgcgc
       61 ggccgggtcg cctcgatcgc gaccagcgtg gccgcgatcg cgcccttggt gtcggccgag
      121 cccagcccgt agagccggcc ctcggacacg tagggcgtcc acggatcgcg cgcccagccg
      181 gcgttggccg gcacggtgtc gacgtgaacg ttgatcagcc ggtgcggcgt gccccagcgc
      241 gcgaacacgt acgcgcccgg gccgccgtcg ctgcgggttg cgatctcgac gatgacctca
      301 tcggcgccgg ccgagcgcag catcggcgcg aggtactcgc acagcgcgcg ctcgtcgccg
      361 gcgccgcgct cggggccggc ctgctgggtc ggaaacgcga tgagctcggc ggtcaggtcg
      421 gcgaccatca tcgttgcacc tcgggttgcg cctcgggatc ggggcggcgg gatcggggcg
      481 cgatgatacg tcggccgcgc gggttgtcag ccgccggtgc gtcaattcct gcgcggcgcg
      541 cccggctcgt cgaggagtga gcgccaggcc gcgtgcgccg cgatctcggc gagcgcctgc
      601 gccagcagcg tcttgcccgg ctccatgcgg tgccaggtcg aggcccccga cgggtgcggc
      661 agcgcgatca cgtcgaactc gacgccggcc agcgcgccgc gccggggctg gccgacgatc
      721 tgcgcgagcg cctcggcgtc cgggaacagc tgtgcgatcg ccagcttgcc gaccgggatc
      781 accaggcgcg ggcgcaggag ctcgacctcc cgggtcaggt gccggcgaca ccgcgcgatc
      841 tcgtcgggcg ctggcacgcg atcgccgccg cccttggcct tgcctggaaa gcaccggcag
      901 accgccgcca tgtacacgcg ctgccggaac gcctcctcct ccaggccgag ccgagcgaac
      961 cagccgaaca tcgtcttgcc cgcggtccac gcgaacggct tgccggccgg gccctcgcgc
     1021 acgccgggcg cctggccgat cagcatcacc ggcgatacca ccggcacccc gctcaccacc
     1081 ggtccgatca tctcggggca agcgtcacat tgccgtagct gtgcgacgtg acgccggagc
     1141 tggaccagga cggacaccgg cgcactatgc ccgatcggtg tgacgccagc accctccgga
     1201 gtcatcccgg cgcacttggc gcaccacgcc aggcgacgtt cacgctgcac ggcgatgccg
     1261 gcacgctaga ctcatcggcg tgaacactcc gccgcatgaa ccgctcatcc gccgggtgct
     1321 cggcggcaac gtcatcgagc tcaagctgca ccggctcgcg atcgaggagc tcgacggcgg
     1381 ctcggtcgtc aagctggagg tcctcgaggg cgatcagccg gtcgaggtcg agggccgcgg
     1441 ggtcggcgtg gtcgaggcgc tgtacaacgc gctgctcgag cgctacgcgc gcgagtacca
     1501 gtcgctcaag acgatccagc tgaccgggtt tcaggtcgca gccgatctcg ggaccaagaa
     1561 ggcgcaggcc ggggtcgacg cggtcgccca ggtcacgctc gacgtcagca acagcgaggg
     1621 ccggctgttc tcgttcagcg actcgtcgcg ctcggtggcg tcgtcgaccg cgcgcgccgt
     1681 gctcgcgatg gtccagtact tcgtcaacgc cgagcgcgcg ttcctcaccc tgtacaacgc
     1741 gcggcgcgac gcgctcgccc gcggccgcga ggacctggtc gcgcgctaca ccgccgagat
     1801 ggccgccgtg gtcgagagca cgagccaggc cgaggtgatc gcgaacatcc gcaaggagct
     1861 cggctag
//
LOCUS       DASVOR010002292         4681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_345635,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002292 DASVOR010000000
VERSION     DASVOR010002292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345635"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            360..674
                     /locus_tag="VF516_42355"
     CDS             360..674
                     /locus_tag="VF516_42355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114468.1"
                     /translation="MVTSGEDELVQGAAGRPFEARTLVGRWRQSAPSSGGGAQAGEDL
                     EPLEAWPELDAVGARYYGAVQGRRLWDMPNNLHFVLANLGVPNDVRDRSDHLRVCPRR
                     DG"
     gene            complement(1260..4655)
                     /locus_tag="VF516_42360"
     CDS             complement(1260..4655)
                     /locus_tag="VF516_42360"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8114469.1"
                     /translation="MGEGGCAVVYRCEQPQLRRSAVVKVLHERGQRSDAALDRFRREA
                     LLASQLDHPYAAHVYAFGDEDDGLFWIAMELVQGIALDDWLEAHGPMPLAQFVPFFEC
                     VADVVHAAHERGIVHRDLKPSNVMVIERNGRLFPKLLDFGIAKLTGEPALPAPERPSD
                     AEAVTTAPLRALRRRAQRTRTDPADQDHRLTRTSAKLGSTAYMSPEQWTNPQAVGAAS
                     DIYSLGILAYEVLTGRKPFTAESTHEYYHQHVYAEVPPLGDAFPPEVDRVIRRALAKS
                     PDARHGSALELAAELRAALQLQPHEQLRSLARVWDARARSPELLVKSKELLLAPTSVV
                     GELERAFVTESRRHAARRARLRRALAGIAVALALGAVWYRGKLQTELAEQQARAARQV
                     TEATITQSELEQGRAALLHNEPEALPHLAEAYRRAPSPSTAFMLARAMQPRLAEQARF
                     QSSFGRMWSATFSPDGTQIVTTDDKNAQVWDAQTYRLRFTLRHGHEVYHCVYSSDGGR
                     IVTAAEDAVRIWDATTGALVYKLTQQRGDGSPTDSFAVAISSDGRLVAALDAAGSFVQ
                     VWDTVRGTPIAHLANDASKFPGLTFSSDGRWLATTGGNDVYVFDTRTWKRALTIRGLR
                     VRGLAFDPTGSRLVTGGTTGDVAIWDVPNSTRLRHLRELGEPVDAVAFSPDGQLIAAA
                     CRDGSAQVWRAGSGALQSQFHARHSKILMMEFDRASQLVLAAGADGTAVVADAFLGMP
                     ITVLEGPRASLQAAHFAPNSKRVIGASWDGTARLWDATSPYRRWSTPPVSDDCGIVTS
                     ADPDRRFIAVGCRNQATHVWDTARGELLAELPSVSRVKGDFASAFPAVSSAGDRAAIA
                     RGNGVEVYQLPGGRLLRTILHAAPVNAVAFADRARDILSGAVDGSLLVTRDGGAQLVL
                     PTAQGEIDAVGFLPDGRIVVSDERRRLRVYDPTGAPLADIELPARTMSLRPDGSRLIA
                     IPLYTSNVTPPLLVDIERYRITAELKGHIGRVFSARWMPNGQILTAGADGTARLWNGL
                     TGQPDQSFQGSSRFLMDATLISTGLLVAGGADGILRFWDVASARLLWALPVHKSWIIG
                     IHIEDGDIVTRGFTGELSKWTLPDPAQVIGTCRNNELCPKVQQ"
BASE COUNT          760 a         1539 c         1603 g          779 t
ORIGIN      
        1 gagatcgtga cgtgaggccg aagaccgcct cagccgccgg ccacggccac cgacgggccg
       61 gttccggttc cgcaggcttt ggcacctgaa tcggtggcca gcaggcgacg acagaggctc
      121 gtcgcctggt ttcgtgggtg atgaaacgtg cagatcgcgt tgtccggggc ctcgctcgcg
      181 atggtcggcg gtcgctgtcc ggccagagat gaactgtgtc cggacgtcgg acagtcgcac
      241 gacgcgcgcc cacgcccggg tatgggcgcg ctcgccacag ccagctgtga gtaaccgtga
      301 gaccgacgag cgcacagcgg cgccgtcgga aatacttgga ggcgaccggc agcgttcacg
      361 tggtcacctc cggcgaggat gagctcgtgc aaggcgctgc cggtcgtcct tttgaggcac
      421 gcacgctcgt gggccggtgg cgccagtcgg cgcccagctc cggcggcggc gctcaggccg
      481 gcgaggacct cgagccgctc gaagcctggc ctgagctcga cgcggtcggc gcgcgctact
      541 acggcgcggt ccaggggcga cggctctggg acatgccgaa caacctgcac ttcgtgcttg
      601 cgaacctggg cgtacccaac gacgtacgag atcgatcgga tcatctacga gtatgcccac
      661 gacgcgatgg ttgagctcgc gacgccgtgg tacgtgttcc aggcggccga tcatgactgg
      721 gtgcgtgaca ccgccggctc gctgcccgag atcgagaagg cgacgctgcg gctggtcgcg
      781 ctttgcgcgc cgcgcaacct cagccacgcc gcgacgcggg atggcccggg tttcgttatc
      841 gctggaccgg ctgccgcgag gtgccgatgg agatcgtgaa atgaggccga agacagcctc
      901 aggggttcgc ggctgccaac gcggtgcacg cggcatcacc cgatccgacg acggggccgg
      961 ttccgcaggc tttggcacct gaatcggtgg tagccagcgg gctggcgaca gagcctcctg
     1021 cggcggctcg gagatcgaca ctgctcagtg tccgtactgc ttctctccgc agtacaagct
     1081 tcttggttac cttcatcatg gttgcacctt ggcgggagca tccgtgcggc agcccgcgtc
     1141 gcctgagtcg cggagttgga tggtaccgga gtcgtcaccc gcggctacat acatgagatc
     1201 tcggttggcc agcgcccggg ggttctctcg acgcagcaca agttttcggt ttaccttcat
     1261 cattgttgca ccttagggca aagctcgttg ttcctgcacg tgccgatcac ctgcgcgggg
     1321 tccggcaagg tccacttcga gagttccccc gtgaatccgc gagtgacgat gtcgccatcc
     1381 tcgatatgga tcccgatgat ccaggacttg tgcacgggga gtgcccagag cagacgcgcg
     1441 ctggccacgt cccagaaccg cagtatgcca tcggcgccac cggccacgag caggccagtg
     1501 gagatgagtg ttgcgtccat caggaatctg gagcttcctt gaaaactctg gtcaggctgc
     1561 ccagtcaagc cattccacag tcgggcagta ccatccgcac cggcggtgag gatctggcca
     1621 ttgggcatcc agcgtgctga aaagactcgg ccgatatgac ccttgagctc agccgtgatt
     1681 cgataacgct cgatgtccac caatagtggc ggtgttacat tgctggtgta aagcgggatt
     1741 gcgatcaagc gcgagccgtc gggccgcagc gacatggtgc gcgctgggag ctcgatgtct
     1801 gccagaggcg cgccggtcgg atcgtagact cgcagtcgtc gtcgttcatc agacacaacg
     1861 atccgaccat cgggcaagaa cccgactgcg tcgatctctc cctgcgcggt tggaagcaca
     1921 agttgcgcgc cgccgtcgcg cgtgacgagc aacgagccgt cgacggctcc gctgaggatg
     1981 tctcgagccc ggtctgcgaa ggccaccgca ttgaccggcg cggcgtgtag gatcgtgcga
     2041 agcagtcgcc cacctggcag ctggtacacc tcgacaccgt tgcctcgggc gattgccgcg
     2101 cgatccccgg cgctggacac tgcaggaaat gccgatgcga agtccccttt tacacgtgac
     2161 acgctcggca gctctgcgag tagctcccca cgtgccgtgt cccacacatg ggtcgcctgg
     2221 ttccggcagc ccacggcgat gaagcggcga tcggggtccg cgctggtgac gataccgcag
     2281 tcatcgctca cgggaggagt gctccagcgg cgatacggcg aagtcgcgtc ccagagccga
     2341 gccgtcccat cccaggatgc gccgatgaca cgcttcgaat tcggggcgaa gtgcgcggct
     2401 tgcaggctcg cgcgcgggcc ctcgagtacc gtgatgggca tcccgaggaa ggcatctgcg
     2461 acgaccgcgg taccatcggc acccgcggcg agcacgagct gggaagctcg atcgaactcc
     2521 atcatgagga tcttgctatg gcgagcatgg aactgacttt gcagtgctcc tgagccagca
     2581 cgccatacct gcgccgagcc atcgcgacac gccgcggcga tgagctgtcc gtcgggcgag
     2641 aaagcaacag cgtcgaccgg ttctccgagc tcgcgcaggt gtcgaagcct tgtgctattc
     2701 gggacgtccc aaattgctac atccccggtc gtaccgccgg tgacgagacg tgaacccgtc
     2761 ggatcgaagg caaggccacg aacgcgaagc ccacgaatgg tgagtgctcg cttccaggtt
     2821 cgcgtatcga atacgtacac atcattccct ccggtcgtgg cgagccagcg accgtcggag
     2881 ctgaacgtca ggccagggaa cttcgaggca tcattggcca gatgtgcgat cggcgtgcct
     2941 cggacggtgt cccagacctg cacgaacgat cctgccgcat cgagcgcagc aacgagccgg
     3001 ccatcggatg agatcgccac cgcgaaggaa tccgtgggcg agccgtctcc gcgctgctgc
     3061 gtcagcttgt acaccagcgc accggtggtg gcgtcccaga tccggaccgc gtcttcggcg
     3121 gccgtgacga tcctcccgcc gtcgctgctg tagacgcagt gatagacctc gtgcccgtgg
     3181 cgcagcgtga accgcaggcg gtaggtctgt gcgtcccaca cctgcgcgtt cttgtcatcg
     3241 gtggtgacga tctgcgtgcc gtccggcgag aacgtcgccg accacatgcg gccgaacgag
     3301 ctctggaatc gcgcctgctc ggcgagccgg ggctgcatcg cgcgcgccag catgaacgcg
     3361 gtcgatggcg atggggcgcg gcggtaggcc tcggcgaggt gaggcagcgc ctcgggctcg
     3421 ttgtgaagca gcgccgcgcg accctgctcc agctcggact gcgtgatggt tgcttcggtc
     3481 acctgccggg ccgcgcgtgc ctgttgctct gcgagctcgg tctgcagctt gcctcgatac
     3541 cagaccgctc ccagcgcgag cgccacggcg atcccggcca gcgcgcgccg gagccgtgcc
     3601 ctgcgcgcgg cgtgtcgccg gctctcggtc acgaatgccc gctcgagctc tccgacgacg
     3661 gaggtggggg ctagaaggag ctccttgctc ttcaccagca gctcgggtga ccgcgcgcgg
     3721 gcgtcccata cccgtgccag cgatcggagc tgctcgtgcg gctgcagctg cagcgccgcc
     3781 cgcagctccg cggccagctc cagcgcgctg ccgtggcgcg cgtcggggga cttggcgagc
     3841 gcgcgccgga tgacccggtc gacctcggga gggaaggcgt cgccaagcgg cggtacctcg
     3901 gcatagacgt gctggtggta gtactcatgc gtgctctcgg cggtgaacgg cttgcgtccg
     3961 gtcagcacct cgtaggcgag gatgccgagc gagtagatgt cgctggccgc gccgacggcc
     4021 tgcgggttcg tccactgctc cggggacatg tacgccgtcg agccgagctt ggcgctcgtg
     4081 cgcgtcaggc ggtgatcctg gtccgcgggg tccgtgcgcg tgcgctgggc gcgccgccgc
     4141 aaggcgcgca gcggcgcggt cgtgaccgcc tcggcgtccg agggacgctc cggcgcaggg
     4201 agcgcgggct cgccggtcag cttggcgatc ccgaagtcga gcagcttggg gaacaggcga
     4261 ccgttgcgct cgatgaccat cacgttcgac ggcttcaggt cgcggtggac gatcccgcgc
     4321 tcgtgggcgg cgtggacgac gtcggcgacg cactcgaaga acggcacgaa ctgcgcgagc
     4381 ggcatcggcc cgtgcgcctc cagccagtcg tcgagggcga tgccctgtac cagctccatc
     4441 gcgatccaga acagaccgtc gtcttcatcg ccgaacgcat agacgtgcgc cgcgtagggg
     4501 tgatcgagct gcgaggcgag cagcgcctcg cgccggaagc gatcgagcgc ggcgtcgctg
     4561 cgctgccccc gctcatgcaa taccttgacg accgcgctgc gccggagctg tggctgctcg
     4621 cagcggtaca ccaccgcaca gccgccctcg ccgatctgct cgcggaggac gaaatccccc
     4681 a
//
LOCUS       DASVOR010002293         3721 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_229464,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002293 DASVOR010000000
VERSION     DASVOR010002293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3721)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3721)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3721
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229464"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="VF516_42365"
     CDS             complement(<1..778)
                     /locus_tag="VF516_42365"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEX8114470.1"
                     /translation="MLEHRLAKLAAAATFVLLVIGGTVNPTGSSLACPEPTLVCHGQL
                     FPPMVGGVLYEHGHRLAAMTVGLLQIALTLVLLRRGSRRLGWILLGLVIAQGALGAIT
                     VGYKLPWFVSTGHLLLGMSYFATLIYTAFRTRPAPTAQELEHHARLRGELGGARRWIA
                     VACGAVFVQLLIGALVRHLGAAMVCLGMPSCTRSGDWWPEAGVQDLHMIHRGFGVVVA
                     IVTTAAAVAIYRRARSWPALRLLALIAPALASAQLALGIYT"
     gene            940..2895
                     /locus_tag="VF516_42370"
     CDS             940..2895
                     /locus_tag="VF516_42370"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8114471.1"
                     /translation="MTDWVETVSIGTFRSEDLAKPGAVFRYNRYLYRTTGKHLSNGGM
                     GTVYELERRDDVTGAIEPVVGKVFHSNYLFQLRTDEITRRDHHNNLAAMARIAAIEHP
                     NILPTYVQAPISDNYLFVTPRMGMTLLEAIAKHNLTPRARTKLLVQALEGLATLHAAR
                     LIHRDFTLRNILVDEGANVAYVFDFDLAMSLDDLGAQTYRGYYKGRIFGSPGWSVAPE
                     TIDQALMDSPINTALDIYAIGGALHGLFTDQLLYGPADDMWALLIRIAEGVVVGGKSK
                     VYYPEGFPLVLRAAIEGCIERDPNQRFPSVQAVVAELRGTLRELPDDRPSRKSSRVVE
                     RTRTNESALKETQDPTITSEIVEAAERAVYDWGYAVERSLGRVRGHPIFLAAPRADMV
                     ESGQFPDANIFPKLVTVIDLTKIADPRKFVENWQQYFWPTLKRVRQGLLTSLHKVIYD
                     ANTSSLLLFSEYVDEPRFGERIAELDLNIDGALALGFVVIRQVAALHEHGMAHNNISP
                     GALLFKGAAATRLVMPAMIGLVEPAMGAEAMAGDVRALAGMILIWLRPNRVASLHVRI
                     KPLFDALRAKLSTWAFDKQIATPKIDELMATISDALALVDFNFSVLRDSGGDLQEYCL
                     LLISLRLYHLLWPSVPSVVPRSTLPPT"
     gene            2892..>3721
                     /locus_tag="VF516_42375"
     CDS             2892..>3721
                     /locus_tag="VF516_42375"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEX8114472.1"
                     /translation="MTTHSSRWWQRWLAVIAVALVAAGFAIAFRGALAAVDRLLGGSG
                     SVAMIEAVPAWERVVLPAVGGLLVGGILLVAARVREGAGVGFVMEAIVLGRVRVPLTR
                     SALQAIASWLAIASGNSLGREGPLIQFGAAAGEGARRVFRLDNITARIVLATGVAAGF
                     ASAYNAPVAAVLFVVEIVTGVVVLEAIVPMLVAVVIATLATRYAVGGAPLYGERAFQL
                     GSPIELLAVAGLGLVAAPVGVGFLRLLGLASRLLRKLPLPWRPAVGGLLCGAILAVLP
                     L"
BASE COUNT          529 a         1341 c         1300 g          551 t
ORIGIN      
        1 cggtgtagat gccgagcgcg agctgggcgg aggccagggc cggcgcgatc agcgcgagca
       61 gccgcagcgc gggccatgac cgggcgcggc ggtagatcgc gaccgccgcg gcggtggtga
      121 cgatcgcgac cacgacgccg aacccgcggt ggatcatgtg gagatcctgc actcccgcct
      181 cgggccacca gtcgccggac cgcgtgcagc tcggcatgcc gaggcagacc atcgccgcgc
      241 cgaggtggcg gaccagcgcg ccgatcagga gctgcacgaa caccgcgccg cacgcgaccg
      301 cgatccagcg ccgggcgccg ccgagctcgc cgcgcagccg cgcgtggtgc tccagttcct
      361 gggcggtcgg cgcgggccgg gtgcggaacg ccgtgtagat gagggtggcg aagtagctca
      421 tgccgagcag caggtggccg gtcgacacga accacgggag cttgtagccg accgtgatcg
      481 cgccgagcgc gccctgcgcg atcaccaggc caagcaagat ccagcccagc cgccgcgacc
      541 cgcggcgcag cagcaccagc gtgagcgcga tctgcaacag gcccaccgtc atcgccgcca
      601 gccggtggcc gtgctcgtac agcacgccgc cgaccatcgg cgggaacagc tggccgtggc
      661 acaccagtgt tggctccggg cacgccaggc tggagccggt cgggttcacg gtcccgccga
      721 tcacgaggag cacgaacgtc gcggctgcag cgagcttggc cagacggtgc tcgagcatgt
      781 gatggtcgct gggtaccaca acgtcgatgg ccgcgcatcc ggtgggcggc gctgcgacaa
      841 gacaccgccg tcgagtggag tcccagcgat atcagcagct tgccgctcgc gaaggttgct
      901 ccgccgacct tcgtcggcgt acagtctcac ccggcgagga tgaccgactg ggtcgaaact
      961 gtgtcgatcg ggacgttcag gtccgaggac ctcgccaagc ccggcgccgt cttccgctac
     1021 aaccgctatc tctaccgcac gaccggcaag cacctgtcca acggcgggat gggcacggtc
     1081 tacgagctcg agcgccgcga cgacgtgacc ggcgcgatcg agccggtggt cggcaaggtg
     1141 ttccactcca actacctgtt ccagctccgc accgacgaga tcacccgccg cgatcaccac
     1201 aacaacctcg ccgcgatggc gcggatcgcc gcgatcgagc accccaacat cctgccgacc
     1261 tacgtccagg cgccgatctc ggacaactac ctgttcgtga ccccgcgcat ggggatgacg
     1321 ctgctcgagg cgatcgccaa gcacaacctg acgccgcgcg cccgcaccaa gctgctggtc
     1381 caggcgctcg agggcctggc gacgctgcac gcggcgcggc tgatccaccg cgacttcacg
     1441 ctgcgcaaca tcctggtcga cgagggcgcc aacgtcgcct acgtgttcga cttcgatctc
     1501 gcgatgtcgc tcgacgacct cggcgcgcag acctaccgcg gctactacaa gggccggatc
     1561 ttcggctcgc ccggctggtc ggtcgcgccc gagaccatcg atcaggcgct gatggactcg
     1621 ccgatcaaca ccgcgctcga catctacgcg atcggcgggg cgctgcacgg cctgttcacc
     1681 gatcagctgc tctacggccc ggccgacgac atgtgggcgc tcctgatccg gatcgccgag
     1741 ggggtggtgg tcggcggcaa gagcaaggtg tactaccccg agggcttccc gctggtgctg
     1801 cgcgcggcga tcgagggctg catcgagcgc gatcccaacc agcggttccc gtcggtccag
     1861 gccgtggtcg ccgagctgcg cggcacgctg cgcgagctgc ccgacgaccg gccgagccgc
     1921 aagtcgagcc gggtcgtcga gcggacccgg accaacgagt cggcgctcaa ggagacccag
     1981 gacccgacga tcaccagcga gatcgtcgag gccgccgagc gcgcggtcta cgactggggc
     2041 tacgcggtcg agcgcagcct gggccgggtc cgcggccatc cgatcttcct cgccgcaccg
     2101 cgcgccgaca tggtcgagag cggccagttc cccgacgcca acatcttccc caagctggtg
     2161 accgtcatcg atctgaccaa gatcgccgat ccgcgcaagt tcgtcgagaa ctggcagcag
     2221 tacttctggc cgacgctcaa gcgcgtccgc cagggcctgc tcacctcgct gcacaaggtg
     2281 atctacgacg ccaacacgtc gtcgctgctg ctgttcagcg agtacgtcga cgagccgcgg
     2341 ttcggcgaac ggatcgcgga gctcgatctc aacatcgacg gcgcgctcgc gctcggcttc
     2401 gtcgtgatcc gccaggtcgc cgcgctgcac gagcacggca tggcgcacaa caacatctcg
     2461 ccgggcgcgc tcttgttcaa gggcgcggcc gcgacccgcc tggtcatgcc ggcgatgatc
     2521 ggcctggtcg agcccgcgat gggcgccgag gcgatggcgg gcgatgtccg cgcgctcgcc
     2581 gggatgatcc tgatctggct gcggcccaac cgggtcgcgt cgctccacgt ccggatcaag
     2641 ccgctgttcg acgcgctgcg cgccaagctg tcgacctggg cgttcgacaa gcagatcgcg
     2701 accccgaaga tcgacgagct gatggcgacg atcagcgacg cgctcgcgct ggtcgacttc
     2761 aacttctcgg tgctgcgcga ctccggcggt gatctccagg agtactgcct tttgctgatc
     2821 agcctccgcc tctaccacct gctgtggccg agcgtgcctt cggtggtgcc ccggtccacg
     2881 ctgccaccga cgtgaccacg cacagctccc ggtggtggca gcgctggctc gccgtgatcg
     2941 cggtggcgct ggtcgcggcc gggttcgcca tcgcgttccg cggcgcgctc gccgcggtcg
     3001 accggttgct cggcggcagc ggcagcgtcg cgatgatcga ggccgttccg gcgtgggagc
     3061 gggtcgtact gccggcggtg ggcggcctgc tcgtcggggg gatcctgctc gttgcggcgc
     3121 gggtccgcga gggcgccggc gtcggcttcg tgatggaggc gatcgtgctc ggccgggtcc
     3181 gcgtgccgct gacccgcagc gccctgcagg cgatcgcgtc gtggctggcg atcgcctcgg
     3241 gcaactcgct gggccgcgag ggcccgctga tccagttcgg cgccgcggcg ggcgagggcg
     3301 cgcgccgggt gttccggctc gacaacatca ccgcgcgcat cgtgctcgcc accggcgtcg
     3361 ccgccgggtt cgcgtcggcc tacaacgcgc cggtcgccgc cgtgctgttc gtcgtcgaga
     3421 tcgtcaccgg cgtggtcgtg ctcgaggcga tcgtgccgat gctggtcgcg gtcgtgatcg
     3481 cgacgctggc gacccgctac gcggtcggcg gcgccccgct gtacggcgag cgcgcgttcc
     3541 agctcggctc gccgatcgag ctcctcgcgg tcgccggcct cggcctggtc gccgcgccgg
     3601 tcggcgtcgg cttcctccgc ctgctcggcc tcgcctcccg gctgctgcgc aagctgccgc
     3661 tgccgtggcg gcccgcggtc ggtggcctgc tgtgcggcgc gatcctcgcg gtgctgccgc
     3721 t
//
LOCUS       DASVOR010002294         2706 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_259253,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002294 DASVOR010000000
VERSION     DASVOR010002294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259253"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2468
                     /gene="carB"
                     /locus_tag="VF516_42380"
     CDS             <1..2468
                     /gene="carB"
                     /locus_tag="VF516_42380"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEX8114473.1"
                     /translation="ASIAIMREIGVTTGGSNVQYAVDPKTGRMLVIEMNPRVSRSSAL
                     ASKATGFPIAKIAAKLAIGYTLDELHNDITRVTPASFEPTIDYVVVKWPRFAFEKFPS
                     ATPVLGPQMKSVGEAMAIGRTFREALGKAIRSLETGRAGFDLPMTALGDDLAMIERAI
                     ATPSPDRLYQIGRGFQLGLTIERAHELTRIDPWFLHHIRGIATAEQALAGSPLARLTA
                     GDLRRYKRMGMSDRRIAALTGAGEAEVRAARHAHGVRPVYKRVDTCGAELESFTPYLY
                     STYEDDCEARPTDQKKVIILGGGPNRIGQGIEFDYCCCHAAMALREEGIESIMVNCNP
                     ETVSTDYDTSDRLYFEPLTLEDVLAICDVEQPWGVIVQFGGQTPLKLAVALAAAGVPI
                     LGTSADAIDRAEDRERFGAVMDKLGLRAPRWGIVRNLDEARRVAGEIGFPVMVRPSYV
                     LGGRAMERVYDARGLEDYFTRVLGSGAPGAGGGGRDESSTVGFPLLIDQFLADAVELD
                     VDVVADHTGAVVVGGVMEHIEEAGIHSGDSACALPPYSLPADLVDEVKRQARMLATEL
                     GVVGLMNTQFAIHDGEIYVLEVNPRASRTVPFVSKAIGKPLAKIAAKVMAGRTLAELG
                     AFPSGEVEPRHVSVKEVVFPFVKFEGVDTILGPEMRSTGEVMGIDTDFARAFMKSWLA
                     SGGRVPTRGTAFLSVRDADKPAVVEIARRLIHLGFELVATHGTAAYLRKAGLDVRGIN
                     KVLEGRPDCVDAMDNREIHLVINTSEGAQAIQDSVSLRRAALMNGIAYQTTLRGARAA
                     LEAIAVAKRGDLRVASLQKYGRL"
     gene            2494..>2706
                     /locus_tag="VF516_42385"
     CDS             2494..>2706
                     /locus_tag="VF516_42385"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rid family hydrolase"
                     /protein_id="HEX8114474.1"
                     /translation="MKRETLNPATLSRPVTYSHVTIITSARQVHISGQVAFNAAGELV
                     GKGDLAAQTEQVYTNLGHALAAAGASL"
BASE COUNT          407 a          974 c          958 g          367 t
ORIGIN      
        1 atgcgtcgat cgcgatcatg cgcgagatcg gcgtgacgac cggcgggagc aacgtgcagt
       61 acgcggtcga tccgaagacc gggcggatgc tggtgatcga gatgaaccca cgcgtgtcgc
      121 gcagctccgc gctcgcgtcg aaggcgaccg ggttcccgat cgccaagatc gccgccaagc
      181 tcgcgatcgg ctacacgctc gacgagctcc acaacgacat cacccgggtg acgccggcgt
      241 cgttcgagcc gacgatcgac tacgtggtcg tcaagtggcc gcggttcgcg ttcgagaagt
      301 tcccgtcggc gacgcccgtg ctcggcccgc agatgaagtc ggtcggcgag gcgatggcga
      361 tcggccggac gttccgcgag gcgctcggca aggcgatccg gtcgctcgag accgggcgcg
      421 ccgggttcga cctgccgatg accgcgctcg gcgacgacct cgccatgatc gagcgcgcga
      481 tcgcgacgcc gtcgcccgac cggctgtacc agatcggccg cggcttccag ctcgggctga
      541 ccatcgagcg cgcccacgag ctgacccgga tcgatccgtg gttcctgcac cacatccggg
      601 ggatcgcgac cgccgagcag gcgctggccg gctcgccgct cgcccggctg accgctggcg
      661 acctgcggcg ctacaagcgg atggggatga gcgatcgccg gatcgccgcg ctgaccggtg
      721 ccggcgaggc cgaggtccgc gccgcgcgcc acgcccacgg ggtgcggccg gtctacaagc
      781 gggtcgacac ctgcggcgcc gagctcgagt cgttcacgcc gtacctgtac tcgacctacg
      841 aggacgactg cgaggcccgg ccgaccgacc agaagaaggt gatcatcctg ggcggcgggc
      901 ccaaccggat cggccagggc atcgagttcg actactgctg ctgccacgcg gcgatggcgc
      961 tgcgcgagga gggcatcgag tcgatcatgg tcaactgcaa ccccgagacc gtgtcgaccg
     1021 actacgacac ctcggaccgg ctgtacttcg agccgctgac gctcgaggac gtgctggcga
     1081 tctgcgacgt cgagcagccg tggggcgtga tcgtgcagtt cggcgggcag accccgctca
     1141 agctcgcggt cgcgctcgcc gccgccggcg tgccgatcct cgggaccagc gccgacgcga
     1201 tcgaccgcgc cgaggatcgc gagcggttcg gcgcggtgat ggacaagctc gggctgcgtg
     1261 cgccgcgctg gggcatcgtg cgcaacctcg acgaggcccg ccgggtcgcc ggcgagatcg
     1321 gcttcccggt gatggtccgg ccgagctacg tgctgggcgg ccgcgcgatg gagcgggtgt
     1381 acgacgcgcg cggcctggag gactacttca cgcgggtgct cggcagcggc gcgccgggcg
     1441 ccggcggcgg tggccgcgac gagtcgtcga ccgtcgggtt cccgctcttg atcgaccagt
     1501 tcctcgccga cgcggtcgag ctcgacgtcg acgtcgtcgc cgatcacacc ggcgcggtcg
     1561 tggtcggcgg cgtgatggag cacatcgagg aggccgggat ccactccggc gactcggcgt
     1621 gcgccctgcc gccctactcg ctgcccgccg acctggtcga cgaggtcaag cgccaggccc
     1681 ggatgctcgc caccgagctc ggcgtggtcg gcctgatgaa cacgcagttc gcgatccacg
     1741 acggcgagat ctacgtgctc gaggtcaacc cgcgcgcgtc gcgcacggtg ccgttcgtca
     1801 gcaaggcgat cggcaagccg ctggccaaga tcgcggccaa ggtcatggcc gggcgcacgc
     1861 tcgccgagct cggcgcgttc ccgtccggcg aggtcgagcc gcgccacgtc tcggtcaagg
     1921 aggtcgtgtt cccgttcgtc aagttcgagg gcgtcgacac catcctcggc ccggagatgc
     1981 ggtcgaccgg cgaggtcatg ggcatcgaca ccgacttcgc gcgcgcgttc atgaagagct
     2041 ggctcgcgtc gggcggccgg gtgccgaccc gcggcaccgc gttcctctcg gtgcgcgacg
     2101 ccgacaagcc cgcggtggtc gagatcgcgc gccggctgat ccacctcggc ttcgagctgg
     2161 tcgcgaccca cggcaccgcc gcctacctgc gcaaggccgg gctcgacgtg cgcggcatca
     2221 acaaggtcct ggagggccgg ccggactgcg tcgacgcgat ggacaaccgc gagatccacc
     2281 tggtgatcaa cacgtccgag ggcgcccagg cgatccagga ctcggtgtcg ctgcgccggg
     2341 ccgcgctgat gaacggcatc gcctaccaga ccacgctgcg cggcgcccgg gccgcgctcg
     2401 aggcgatcgc ggtggccaag cgcggcgacc tccgggtcgc ctcgctccag aagtacggcc
     2461 ggctgtagca gcccccaaag ccaggagccc accatgaagc gcgagaccct caaccccgcc
     2521 acgctgtcca ggcccgtgac ctactcccac gtcacgatca tcaccagcgc ccgccaggtc
     2581 cacatctccg gccaggtcgc attcaacgcc gccggcgagc tggtcggcaa gggcgacctc
     2641 gccgcccaga ccgagcaggt ctacaccaac ctcggccacg cgctcgccgc cgccggggcg
     2701 agcctg
//
LOCUS       DASVOR010002295         5825 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_141708,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002295 DASVOR010000000
VERSION     DASVOR010002295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141708"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VF516_42390"
     CDS             <1..494
                     /locus_tag="VF516_42390"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEX8114475.1"
                     /translation="RVAVIGDGKLGLLCAMVLAAHGARPLLIGKHPTKLALADRRGIE
                     TADVAQARQRGRSFDVVVEASGGATGFELALELLQPQGTLVLKSTFHGLTPVDAARVV
                     VEEITVVGSRCGRFAPALGLLARGAVDVADLVSEILPLDRGEAALARAAEPGVLKVLL
                     DAR"
     gene            complement(510..1625)
                     /locus_tag="VF516_42395"
     CDS             complement(510..1625)
                     /locus_tag="VF516_42395"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX8114476.1"
                     /translation="MRGSVFATLLLASCAMGGNPADDSRAGAGLGSDVDSAGALEVGI
                     LARVCAAGATTKGVDVSYFNGTIDWARVKAAGNEFAFIRISDGTGFHDPQFAANWAGA
                     QAVGMVRGIYQFFRPGQDVNAQADMVIAAAGTPAPGDLPPVIDVEVTGNLSPASVAAK
                     VRAWVDRVKAGIGVDPIVYTGKFFWRDQVGGPTSFAGNPLWIAQYTTLCPDLTSPWDT
                     WAFWQYSESGSVAGMSGAVDLDRFNGSLDELRALAGGIGGGGGGGGGGGATSCASATM
                     AADEPEGVCVQAASDQKWYQCTAGSWIQQASTAGCTQTFGWCQSATLGRAVPPRTCVQ
                     KSADRVWYQCNGTTWVTPVDAAAATGPAGACSHEYPL"
     gene            2314..3675
                     /locus_tag="VF516_42400"
     CDS             2314..3675
                     /locus_tag="VF516_42400"
                     /inference="COORDINATES: protein motif:HMM:NF014691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEX8114477.1"
                     /translation="MRSVGARLYTSIPGDKLSGAIRAGWMDIVSALYPDALVSHRTAL
                     EFRPSVDGDIWLTSSTNRVVSYPGLRLRFVRGPGPRADDPTVAGIRSSSRARAFLENL
                     ATTHPSSRTRSIPLAELEQRLEQLLHLDGEPALNELRDRARQIADELGWRREFTRLDT
                     LIGSLLGTRTAQLTSGVGRARAAGEPFDAACQARLQALFAELHGRPLPRIVERFDAPA
                     HVRNKAFFEAYFSNYIEGTTFEIEEAERIVFDQQIPARRPKDGHDIAATFHLVSDPVE
                     MRATPHDFDPLLQLLQTRHRTLMAERPEAEPGAFKQPPNRAGDTHFVDPAYVRGTLRK
                     GMELYLALSPGLARAIFLMFLVTDVHPFVDGNGRIARIMMNAELVSAGQPTIIIPTVY
                     RDDYLLALRALTRRHRPAPLIEALTRAQRFSHLDFSSYPSILQELQRRNWFHEPDEAR
                     RID"
     gene            3843..4184
                     /locus_tag="VF516_42405"
     CDS             3843..4184
                     /locus_tag="VF516_42405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8114478.1"
                     /translation="MISPFAAERIALAELLRSEGYEVTAVADRSEGLALAGAERPDAV
                     IADAQVPGLDGRALVRDLAARGPMPRVILLCPRAGRALESDDVTCLTKPIDLGALHHL
                     LARLAAGPRSA"
     gene            4181..5242
                     /locus_tag="VF516_42410"
     CDS             4181..5242
                     /locus_tag="VF516_42410"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8114479.1"
                     /translation="MTHAAHDVLDLIDGLELLICGLDRRGTIRVFNWPCERLTGISRS
                     DASGRSWLEIFAPGERGDHVVALWSQAREDAPAGPYEALCQNSRSIRWHFCRWDRGRI
                     PEVALWAVGVDLTQDREALVRARELERIVALGNLVSGLTHELRNPLNGALLQLALAER
                     SLARCGGEAVAPAASAIAQASVEIRRISTLLDDFLVFVRPQPIRLERSDVRALAARAV
                     ERGQPKAVAAGVSVVLEPGREAIAAVDGSRVETAVFQLLANAIDAASVSADHEVRVRV
                     GAPGNAVTIEVEDHGAGLPSDAPIFEPFFTTKQGGTGLGLAIVQRVAADHGGRILHEQ
                     RPGATVFRLELPIAGGVAS"
     gene            5485..>5825
                     /locus_tag="VF516_42415"
     CDS             5485..>5825
                     /locus_tag="VF516_42415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114480.1"
                     /translation="MLAAGVDNPALIARWRAEPELLRGHGVDPSQVDLDAMWRFAGLT
                     VKVRHHALREELPTSFRLMNLTGLEIELFASYASERAAQGAKLAPTTEGRTLDLLGFL
                     KGWLDRDNTAHA"
BASE COUNT          855 a         2067 c         2063 g          840 t
ORIGIN      
        1 cgcgggtcgc ggtgatcggc gacggcaagc tcggcctgct gtgcgcgatg gtgctcgcgg
       61 cccacggggc gcggccactg ctgatcggca agcacccgac caagctggcg ctggcggacc
      121 ggcgtggcat cgagaccgcg gacgtggcgc aggcgcggca gcgcggccgg agcttcgacg
      181 tcgtggtcga ggcgtcgggc ggcgcgaccg gcttcgagct ggcgctggag ctgctgcagc
      241 cgcagggcac gctggtgctg aagtcgacgt tccatggcct gacgccggtc gacgcggcgc
      301 gggtggtggt cgaggagatc accgtggtcg gctcgcgctg cggccggttc gcgccggcgc
      361 tcggtctgct ggcgcgcggc gcggtcgacg tcgcggatct ggtcagcgag atcctgccgc
      421 tcgatcgcgg cgaggcggcg ctggcgcgcg cggcggagcc cggcgtgctg aaggtgctgc
      481 tggacgcgcg gtagcggtca ggcgccggct cacagcgggt actcgtgcga gcacgccccg
      541 gccgggccgg tggccgcggc ggcgtcgacc ggcgtgaccc acgtcgtgcc gttgcactgg
      601 taccacaccc gatccgcgga cttctggacg caggtgcgcg gtggcaccgc gcggcccagc
      661 gtggccgact ggcaccagcc gaaggtctgc gtgcacccgg cggtcgacgc ctgctggatc
      721 cagctgccgg cggtgcactg gtaccacttc tggtccgagg ccgcctgcac gcacacgccc
      781 tcgggctcgt cggcggccat cgtcgccgac gcgcagctcg tggcgccgcc gccgccgcca
      841 ccaccgccgc caccgccgat cccgccggcg agcgctcgca gctcgtcgag cgagccgttg
      901 aaccgatcga gatcgacggc gccggacatc cccgcgacgc tgccgctctc gctgtactgc
      961 cagaacgccc aggtgtccca cggcgaggtc aggtcggggc acagcgtggt gtactgcgcg
     1021 atccacagcg ggttgccggc gaacgacgtc ggcccgccga cctgatcgcg ccagaagaac
     1081 ttgccggtgt agacgatcgg atcgacgccg atgccggcct tgacccggtc gacccaggcg
     1141 cggaccttgg ccgccacgct cgccggcgac aggttgccgg tgacctcgac gtcgatcacc
     1201 ggcggcaggt cgccgggcgc cggggttccg gccgcggcga tcaccatgtc ggcctgggcg
     1261 ttgacgtcct ggccggggcg gaagaactgg tagatgccgc gcaccatccc gaccgcctgc
     1321 gcgccggccc agttggccgc gaactgcgga tcgtggaagc cggtgccgtc ggagatccgg
     1381 atgaacgcga actcgttacc ggcggccttg accctggccc agtcgatcgt gccgttgaag
     1441 tacgacacgt cgacgccctt ggtcgtcgca ccggcggcac acacgcgggc gaggatgccg
     1501 acctcgagcg cgcccgcgct gtcgacatcc gagccgagcc cggcgccggc cctggagtcg
     1561 tcggcgggat tgcctcccat cgcgcaggag gccagtagca gcgtcgcgaa gaccgatcca
     1621 cgcatgtttg ttctccgatc agcgtcgtcg ccgggtcagc aagcgacgtg ccctcgcgcg
     1681 tcgcatctgc gcggagcgga cccgcgacgc tccgacggca tcacgcgccg gcggctgcac
     1741 ggatcgtgca gcggcgatcg caccacgcga cggcggcgtg gcggctaccg cggttgccgt
     1801 gggggcgcat gcctcgcgcg aggaggtgcc ggctcccatg ccggcccgtg cccgagccgc
     1861 atcggagcgc cgtcgccggc tccggaccgg tccagaacgg acctacgcct tctcagcatc
     1921 ccgcgggctc caccagcgcg gcgcagagcc gtaccgcccc cggaagcagt cccaagaaat
     1981 cgaccaagaa tcgatcagat cactgtaaat ataccaaaaa ttgtttcatg tcacataaaa
     2041 tggaccaaaa attgtacaaa accgaacaaa tagaccaaaa atcgatccaa atcctggaaa
     2101 tgaaccaaat ggcgtgcgat ctcgcagaga ccgcctgcca tggcacggcc gcgacccaag
     2161 aagccgagga ctgtccgcag tccgtcgagg acgacgccca ggggcgccgg cgccgaagcc
     2221 gggcgctcgg acgtcccgct tcctccgttg ctgtttccgg gcaagaccgc cccggaaaaa
     2281 cgctggcttg ccggcctgaa gaaggcgggg cgggtgcgct ccgtcggagc ccggctctac
     2341 acgtcgatcc cgggcgacaa gctgagcggt gcgatccgcg ccggctggat ggacatcgtg
     2401 tccgcgctgt acccggacgc tctggtcagc caccgtaccg cgctcgagtt caggccgagc
     2461 gtcgatggtg acatctggct gacctcgtcc accaaccgcg tggtctcgta ccccgggctt
     2521 cggctccggt tcgttcgagg ccctggaccg cgcgccgacg atccgacggt cgccggcatc
     2581 cggtcgtcgt cgcgggcacg cgcgttcctg gagaacctcg cgaccaccca cccgagctca
     2641 cgcacccggt cgattccgct ggccgagctg gagcagcggc tggagcagct cctgcacctc
     2701 gacggcgaac cggcgctcaa cgagctccgc gaccgcgcgc gccagatcgc ggacgagctc
     2761 ggctggcggc gtgagttcac gcggctcgac acgctgatcg gcagcctgct cgggacgcgg
     2821 acggcgcagc tgaccagcgg cgtcgggcgc gctcgggccg ccggcgagcc attcgatgcc
     2881 gcgtgccagg cgcggttgca ggcgctgttc gcagagctgc acggccgccc gctgccgcgg
     2941 atcgtcgagc gcttcgatgc tccggctcac gtccggaaca aggccttctt cgaagcgtac
     3001 ttctccaact acatcgaagg aacgacgttc gagatcgagg aggccgagcg gatcgtcttc
     3061 gaccagcaga tccccgcccg gcgtcccaag gatggtcatg acatcgccgc gaccttccat
     3121 ctggtctccg acccggtcga gatgcgagcc acgccgcacg acttcgaccc gctgttgcag
     3181 ctgctccaga cgcgccaccg cacgctgatg gcggagcggc cggaagccga gccgggtgcg
     3241 ttcaagcagc cgcccaatcg cgccggcgac acgcacttcg tggaccccgc gtacgtccgc
     3301 gggacactcc gcaagggaat ggagctctac ctggcactgt ccccgggcct cgcgcgcgcg
     3361 atcttcctca tgttcctggt caccgacgtc catccgttcg tcgatggcaa cgggcggatc
     3421 gcccggatca tgatgaacgc ggagctggtg tcggccggtc agccgacgat catcatcccc
     3481 acggtctatc gggacgatta cctcctggcg ctgcgcgcgc tgacgcggcg ccatcgcccg
     3541 gctccgctga tcgaggcgct gaccagggcg cagcgatttt ctcacctcga cttttcttcg
     3601 tacccgtcga tcttgcagga gctgcagcgc cgcaactggt ttcacgaacc cgacgaggcc
     3661 cgccggatcg actgacactc ggcgtccagc ccggtggggt ggccgggccc actgccgcga
     3721 tgacgcgcca gaaacgcagc ggtgatctcg ccggacgcag ggattgcggg aacccggccg
     3781 gcccgcggcc cgggtcgccg gcatgcccgg tgctactgtg tccggacaag atgcgcagcc
     3841 tggtgatctc cccgttcgcc gccgagcgca tcgcccttgc ggagctcctg cgcagcgaag
     3901 gctacgaggt caccgcggtg gcggatcgct cggaaggcct ggcgctcgcg ggcgccgagc
     3961 ggcccgacgc agtgatcgcc gatgcgcagg tgccggggct cgacggacgg gcgctggtgc
     4021 gcgacctcgc ggcgcgcggt ccgatgccgc gcgtgatcct gctgtgcccg cgcgcgggcc
     4081 gggcgctgga gtccgacgat gtcacctgcc tgaccaagcc gatcgacctc ggcgcgctcc
     4141 accacctgct cgcgcggctc gccgccggac cgcggtccgc atgacacacg cggcgcacga
     4201 cgtgctcgac ctgatcgacg gcctggagct gctgatctgc gggctcgacc gccgcggcac
     4261 gatccgcgtg ttcaactggc cgtgcgagcg gctgaccggg atctcgcgca gcgacgcgtc
     4321 ggggcggagc tggctggaga tcttcgcgcc cggcgagcgc ggcgatcacg tcgtcgcgct
     4381 gtggagccag gcccgcgagg acgcgccggc cggcccgtac gaggcgctgt gccagaacag
     4441 ccggagcatt cgctggcact tctgccgctg ggaccgcggc cggatccccg aggtcgcgct
     4501 gtgggcggtc ggggtcgacc tcacgcagga tcgcgaggcg ctggtccgcg cccgcgagct
     4561 cgagcgcatc gtcgcgctcg gcaacctggt ttccggactg acccacgagc tgcgcaaccc
     4621 gctcaacggc gcgctgctcc agctcgcgct cgccgagcgc agcctcgcgc gctgcggcgg
     4681 cgaggcggtc gcaccggcgg cctccgcgat cgcgcaggcc agcgtcgaga tccggcggat
     4741 ctcgacgctg ctcgacgact tcctggtgtt cgtgcgaccg cagccgatcc gcctcgagcg
     4801 cagcgacgtg cgagcgctcg cggcgcgcgc ggtggagcgc ggccagccca aggcggttgc
     4861 ggccggggtg tcggtggtcc tggagccggg ccgcgaggcg atcgcggcgg tcgacggctc
     4921 gcgggtcgag accgcggtgt tccagctgct cgccaacgcg atcgacgcgg catcggtgtc
     4981 ggccgatcac gaggtgcggg tccgggtcgg tgcgccgggc aacgcggtga cgatcgaggt
     5041 cgaggaccac ggcgccgggt tgccgtcaga cgcgccgatc ttcgagccgt tcttcacgac
     5101 caagcagggc ggcaccggcc tgggcctggc gatcgtccag cgcgtcgccg ccgatcacgg
     5161 cggccggatc cttcacgagc agcgaccggg tgcgacggta ttccggctgg agctgccgat
     5221 cgccggaggc gtcgccagct gaccgcgccg ggagcgcatg cgatcctgcg gcggtgcgcg
     5281 cgagagccgg ttctgtcccg cttgtgaatc ggacgtcctt gacagcgtgc gacggcgcac
     5341 gctacggtca agggcaacga tcatggggta tcgtgacatt acatgtcgcg atcctggcca
     5401 tctccgtagt ggaaggtagg acatgctcag gaagaccaat cgatcggtcg ctcgatgggg
     5461 atgagcgcgc gacggatcca aacggtgctc gccgccggcg tcgacaaccc ggcgctgatc
     5521 gcacgctggc gcgccgagcc cgagctgctg cgcggtcacg gcgtcgatcc atcgcaggtc
     5581 gatctcgatg cgatgtggcg gttcgccgga ttgacggtca aggtccgcca ccatgcgctg
     5641 cgtgaggagc taccgacgtc gttccggttg atgaacctca ccggactgga gatcgagctg
     5701 ttcgcgagct acgccagcga gcgcgccgca cagggcgcca agctcgcacc caccaccgag
     5761 ggccgcacgc tcgatctgct ggggttcctc aagggctggc tcgaccgcga caacaccgcg
     5821 catgc
//
LOCUS       DASVOR010002296         2145 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_403902,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002296 DASVOR010000000
VERSION     DASVOR010002296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403902"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..943)
                     /locus_tag="VF516_42420"
     CDS             complement(<1..943)
                     /locus_tag="VF516_42420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114481.1"
                     /translation="MPWLSACAIAVLGASFIIGLIACSGAERHPAPQKVPVTWRDVRD
                     SAGHTAHVVQRGVPCRDCHGDDGFQPPPLEVCSKCHAQVRAPLHPADPLNKAPAPECQ
                     DCHGFGAKLDVTPTNCMRCHTQPQGGHAAVGAHSKQSCSDCHRAHGSPTLDPRPCTSC
                     HASEDNHHAGARSCLDCHQMHEADAVADQGCATCHARQKGKIKVDDRAITAGHTACTG
                     CHAPHQFDKAQTVACTSCHRKQHTFVPDKHAGCTSCHAQHKASQPKPCTSCHARSVSH
                     PPAARGGPAAACDGCHPPHTLAAGQLAVACATCHDKPH"
     gene            complement(955..1998)
                     /locus_tag="VF516_42425"
     CDS             complement(955..1998)
                     /locus_tag="VF516_42425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114482.1"
                     /translation="MTVRNLAIGLVLCGLAACAGVLGLSRSGTRAFPHRSHVVGGVSC
                     TRCHAGLETRPAGLHLPDDASCTAGGCHARPHDTRPCLTCHVAPSAIAQLTEARDHLK
                     FDHRRHLDGTRGNCMRCHNGVADGDDHMRPAMATCFACHADDAQRDARKCDACHKDLA
                     DEGTLPQTHLAHDGDWLREHGTRAASSGDVCQSCHKPAYCAECHGKTAASLPATQRFA
                     DPFAASVHRAGFEARHSLEARSSPGSCQTCHSPDRCVACHTARGVAGGSTGSPHPPGW
                     VGLTASLNLHGREARRDPAACASCHGGAGETLCVQCHAVGGVGGNPHPPGWSSRQPMS
                     ALPCRMCHPVGSR"
BASE COUNT          230 a          728 c          878 g          309 t
ORIGIN      
        1 ggtggggctt gtcgtggcac gtcgcgcacg cgaccgcgag ctgtcccgcg gcgagcgtgt
       61 gcggcgggtg gcagccgtcg cacgccgcgg cggggccgcc cctggccgcc ggcgggtggc
      121 tgaccgacct ggcgtggcag ctggtgcacg gctttggctg ggacgccttg tgctgggcgt
      181 ggcagctggt gcagccggcg tgcttgtcgg ggacgaaggt gtgctgcttg cgatggcagc
      241 tggtgcacgc cacggtctgg gccttgtcga actggtgcgg cgcgtggcag ccggtgcacg
      301 cggtgtggcc ggcggtgatc gcgcgatcgt cgaccttgat cttgcccttc tgccgggcgt
      361 ggcaggtcgc gcagccctgg tcggcgaccg cgtcggcctc gtgcatctgg tggcagtcga
      421 ggcagctgcg cgcgccggcg tggtggttgt cctcgctggc gtggcagctc gtgcacggtc
      481 tgggatcgag ggtcggcgag ccgtgggcgc ggtggcagtc cgagcagctc tgcttgctgt
      541 gcgcgccgac cgcggcgtgg ccgccctgcg gctgggtatg gcagcgcatg cagttcgtcg
      601 gggtgacgtc gagcttggcg ccgaagccgt ggcagtcctg gcactcgggc gccggcgcct
      661 tgttcagcgg atcggccggg tgcagcggtg cgcggacctg ggcgtggcat ttgctgcaca
      721 cctcgagcgg cggcggctgg aagccgtcgt cgccgtggca gtcgcggcac ggcacgccgc
      781 gctggacgac gtgcgcggtg tggccggcgc tgtcgcgcac gtcgcgccag gtcaccggga
      841 ccttctgcgg cgcgggatgg cgctcggcgc cggagcacgc gatcagtccg atgatgaacg
      901 aggcgccgag cacagcgatc gcgcacgcag agagccaggg caaggtgatg ggcttcatcg
      961 cgaccccacg gggtgacaca tccggcacgg cagggccgac atcggctgcc ggctcgacca
     1021 gccgggcggg tgtggattgc cgccgacgcc gccgaccgca tggcactgca cgcacagggt
     1081 ctcgccggcg ccgccgtggc agctcgcgca cgccgccgga tcgcggcggg cctcgcggcc
     1141 gtgcaggttg aggctcgcgg tcagcccgac ccagcccggc gggtgcgggc tgccggtcga
     1201 tccgccggcg acgccgcgcg cggtgtggca cgcgacgcag cgatccgggc tgtggcaggt
     1261 ctggcagctg cccggcgacg acctggcctc caggctgtgg cgcgcctcga acccggcgcg
     1321 gtggacgctg gcggcgaacg gatcggcgaa ccgctgggtc gccggcaggc tcgccgcggt
     1381 cttgccgtgg cactcggcgc agtacgccgg cttgtggcag ctctggcaga cgtcgcccga
     1441 cgacgccgcg cgggtgccgt gctcgcgcag ccagtcgccg tcgtgcgcga ggtgggtctg
     1501 cggcagcgtg ccctcgtcgg cgaggtcctt gtggcaggcg tcgcacttgc gggcgtcgcg
     1561 ctgggcgtcg tcggcgtggc acgcgaagca ggtcgccatc gccggccgca tgtggtcgtc
     1621 gccgtcggcg acgccattgt ggcagcgcat gcagttgccc cgggtgccgt cgaggtggcg
     1681 gcggtggtcg aacttgaggt gatcgcgggc ctcggtgagc tgcgcgatcg cgctgggcgc
     1741 gacgtggcag gtcaggcacg gcctggtgtc atgcggtctg gcgtggcagc cccccgccgt
     1801 gcagctggcg tcgtcgggca gatggagccc ggccgggcgg gtctcgagcc cggcgtggca
     1861 ccgcgtgcac gacacgccgc cgaccacgtg gctgcggtgc gggaacgcgc gcgtccccga
     1921 gcgcgacagg ccgagcaccc cggcgcaggc ggccagcccg cacagcacca ggccgatggc
     1981 gaggttgcga accgtcatgg cttcccccag cgccggctga gctggaacag cccgtcgacg
     2041 cggctgcgat actcgggcga cgcgctggcc tcgatggcgg cggccgcctg ccagtcgccg
     2101 cggtcccagc tgagcgcggc gagcgcccac ggccacagcg agccg
//
LOCUS       DASVOR010002297         1600 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_317313,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002297 DASVOR010000000
VERSION     DASVOR010002297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317313"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="VF516_42430"
     CDS             complement(<1..1388)
                     /locus_tag="VF516_42430"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HEX8114483.1"
                     /translation="MLQQLTDQDSIFLYGETAETPAHVGGLSLVDLPDGYRGDFFADY
                     KATIARRMALVPFLHTRVARVPLDLDRPFWVEDEHVDLDHHVRRATVPPPGTMAELEA
                     LVALLHQQPLDRTRPLWEFTVIDGLASGQLAIYTKMHHAAMDGGGSQQLITTMYDPTP
                     VPRVLACPELDTAHHRPSARNLVTSLLARRVQQAIRAVQFVPDLLQAVSRVVLPDAET
                     LRFRPIHRMPRTPTTLLNVGITSQRVYAARTLPLPEVKRLAKLTGTTVNDVVLAVCSG
                     ALRDYLADKRRLPGRSLTAMVPVSLRAPGDYARRNQNAMVICSLASDIADPHERLLAI
                     HRSMVAQKQNIEIWKDLPVPDVVIPGLGRIVRRLVELYGHSRFAGRPPLAGNLVISNV
                     PGPPAPLYIAGARIASMYPCSIPFHGQAVNITVESYCDRLDVGLIACRRTVPDVAQLA
                     DRLAGALVELQRA"
     gene            complement(1373..>1600)
                     /locus_tag="VF516_42435"
     CDS             complement(1373..>1600)
                     /locus_tag="VF516_42435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114484.1"
                     /translation="QVAAAQDRLTRRSAGKRCSPAAARRSGCENARGGAIAGWHIPCC
                     SLLRERRPTRPTIAALLDAIEERRGRACCSS"
BASE COUNT          229 a          552 c          589 g          230 t
ORIGIN      
        1 gcacgctgca gctcgaccag cgcgccggcc aggcggtccg ccagctgcgc gacgtccggc
       61 acggtccgcc ggcacgcgat cagcccgaca tcgagccggt cgcagtagct ctccaccgtg
      121 atgttcaccg cctggccgtg gaatggaatc gagcacggat acatcgacgc gatccgcgcg
      181 ccggcgatgt acagcggggc cggcgggccg gggacattcg agatcacgag gttacccgcg
      241 agcggtggac gcccggcgaa ccggctgtgg ccgtagagct cgaccagccg gcgcacgatc
      301 cgccccaggc cgggaatcac cacatccggg accgggaggt ctttccagat ctcgatattc
      361 tgcttctgcg cgaccatgga gcggtggatc gcgagcaggc gctcgtgggg atccgcgatg
      421 tcgctggcca ggctgcagat caccatggcg ttctggttgc gccgcgcgta gtcgcccggc
      481 gcgcgcagcg acaccggcac catggcggtg agcgagcgcc cgggcagcct gcgcttgtcg
      541 gcgagatagt cgcgcaacgc gccgctgcac accgccagca cgacgtcgtt gaccgtggtg
      601 ccggtcagct tggcgagccg cttgacctcg ggcagcggca gcgtccgcgc cgcgtacacc
      661 cgctggctgg tgatcccgac gttgagcagc gtcgtcggcg tgcgcggcat ccggtggatc
      721 gggcggaacc gcagggtctc ggcgtcgggc agcaccacgc ggctcaccgc ctggagcagg
      781 tcgggcacga actgcacggc gcggatcgcc tgctggacgc ggcgcgcgag caggctggtc
      841 acgaggttcc gggcgctggg acggtggtgg gcggtgtcga gctccgggca cgccagcacc
      901 cgcggcaccg gcgtcggatc gtacatggtc gtgatcagct gctggctccc accgccgtcc
      961 atcgcggcgt gatgcatctt ggtgtagatg gcgagctggc cggacgcgag cccgtcgatc
     1021 acggtgaact cccacagcgg ccgggtccga tcgagcggct gctggtggag cagggcgacc
     1081 agcgcctcga gctcggccat cgttccgggc ggcggcacgg tcgcgcgccg gacgtggtga
     1141 tcgagatcga cgtgctcgtc ctcgacccag aacgggcgat cgaggtcgag cggcacccgc
     1201 gcgacccggg tatgcaggaa cggcacgagc gccatgcggc gcgcgatcgt cgccttgtag
     1261 tcggcgaaga agtcgccgcg gtaaccgtcc ggaaggtcga ccaggctcag cccgccgacg
     1321 tgcgccgggg tctctgcggt ctcgccgtac agaaagatcg agtcctgatc ggtcagctgc
     1381 tgcagcatgc acggcccctt ctctcctcga tggcgtcgag gagcgccgcg atcgtcggcc
     1441 gggtcggccg gcgctcgcgc agcaggctgc agcacggtat gtgccagccg gcgatggcgc
     1501 caccgcgcgc gttctcgcaa ccgctgcgcc gcgccgccgc cgggctgcaa cgcttgccgg
     1561 cgctgcgccg cgtcagccgg tcttgagctg cagcgacttg
//
LOCUS       DASVOR010002298         2346 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_171028,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002298 DASVOR010000000
VERSION     DASVOR010002298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2346
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171028"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VF516_42440"
     CDS             complement(<1..654)
                     /locus_tag="VF516_42440"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEX8114485.1"
                     /translation="MSSAATRHFELGLLALAALAALVLAGIVLGLRGLGAATVDYHTY
                     FDESVQGLEIGAPVKYRGVRIGNVSSVAIAPDKKHVAVGLALIARDAQRLGLAAANPE
                     LRTQLSSQGLTGVKFVEIDFADPAANPPPVLPFPPGPRYVPARTSTIKSLEESLQGIG
                     ERLPDLVDHAVSALTKLESLLDELRGDAVPRRLADAIAGVGAAAGDIRRLAQHLDRAR
                     "
     gene            complement(651..1448)
                     /locus_tag="VF516_42445"
     CDS             complement(651..1448)
                     /locus_tag="VF516_42445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8114486.1"
                     /translation="MTGDPEPTPEPTPGPGPVPGPGPMIEVRDLSIGWSRNDVLLEHA
                     SFEVRRGEIFGILGRSAAGKSTLFRVLVGLEQPLTGEVVIAGLGPPRLVGERPAFGVM
                     FQQGALISSLTVGENVALPLARWTELPRDAIRALVSAKLQLVGMEDAEHLLPAELSGG
                     MRKRAAIARALALEPGLVFLDEPSSGLDPVTSAELDHLIATLARTLGLTVVVVTHELG
                     SILAIVDRCILLDRTTRTILAEGPPGELVASPIPHVRHFFARLPEAV"
     gene            complement(1445..>2346)
                     /locus_tag="VF516_42450"
     CDS             complement(1445..>2346)
                     /locus_tag="VF516_42450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8114487.1"
                     /translation="RGVRCLITTGSESQRALVHLYRGDERPRVAEVRRRERGIARLGG
                     AVEHALWQAREMVAFLGELIAAVAAAVRRPAKVSWHDLPGLIVQAGTDGIPIVLVLNF
                     LIGFVMAYQAARQLALFGANIYAADLVGLSVPRELAPLMTAIIIVGRSGAAYAAELGT
                     MRVSDEIDALRTMGFAPVPYLVVPRVLALVVVAPVLTLLGDVVGVIGGIIVGQASLDI
                     TPAGFLAELRTAVEASDVWTGLCKSMAFGAAIGLIGCQHGLAARGAASGVGRGTTSTV
                     VSCLFSIVVIDTLLTMLFRRFGV"
BASE COUNT          346 a          872 c          859 g          269 t
ORIGIN      
        1 ccgcgcgcga tcgaggtgct gggcgagccg gcggatgtcg ccggccgccg cgccgacccc
       61 ggcgatcgcg tcggcgagcc ggcgcggcac ggcatcgccg cgcagctcgt cgagcaagct
      121 ctcgagcttg gtcagggccg acaccgcgtg gtcgaccagg tcgggcagcc gctcgccgat
      181 cccttgcagg ctctcctcga ggctcttgat cgtcgaggtc cgcgccggca cgtagcgcgg
      241 gccgggcggg aacggcagca ccggcggcgg gttcgcggcc gggtcggcga agtcgatctc
      301 gacgaacttc acgccggtga gcccctggct cgacagctgg gtgcgcagct cggggttggc
      361 cgcggcgagc ccgagccgct gggcatcgcg ggcgatcagc gcgaggccga ccgcgacgtg
      421 cttcttgtcc ggggcgatcg cgaccgacga cacgttgccg atccggacgc cgcggtactt
      481 gaccggcgcg ccgatctcca gtccctggac cgactcgtcg aagtaggtgt ggtagtcgac
      541 cgtcgccgcc cccaggccgc gcagcccgag cacgatcccg gccagcacca gcgccgccag
      601 cgcggcgagc gcgagcaacc cgagctcgaa gtggcgggtc gcggccgagc tcataccgcc
      661 tccgggagcc gcgcgaagaa gtgccggacg tgcgggatgg ggctggccac gagctcgccc
      721 ggcggaccct cggcgaggat ggtccgggtc gtgcgatcga gcaggatgca gcggtcgacg
      781 atcgcgagga tgctgccgag ctcgtgggtg accaccacga cggtcaggcc gagcgtgcgg
      841 gccagggtcg cgatcaggtg gtcgagctcc gccgaggtca ccggatcgag gcccgacgac
      901 ggctcgtcga ggaacaccag ccccggctcg agggccaggg cgcgcgcgat cgccgcgcgc
      961 ttgcgcatgc cgcccgacag ctcggccggc agcaggtgct cggcgtcctc catcccgacc
     1021 agctggagct tggcgctcac cagcgcgcgg atcgcgtcgc gcggcagctc ggtccagcgc
     1081 gcgagcggca gcgcgacgtt ctcgcccacg gtcagcgagc tgatcagcgc gccctgctgg
     1141 aacatcacgc cgaacgccgg gcgctcgccg accagccgcg gcgggcccag gcccgcgatc
     1201 acgacctcgc cggtgagcgg ctgctccagc ccgaccagca cgcggaacag cgtggacttg
     1261 ccggccgcgc tgcgccccag gatgccgaag atctcgccgc gccggacctc gaagctcgcg
     1321 tgctccagca ggacgtcgtt gcggctccac ccgatcgaca gatcgcggac ctcgatcatg
     1381 ggcccgggcc cgggcacggg cccgggcccg ggcgtgggct cgggcgtggg ctcgggatcg
     1441 cccgtcatac cccgaacctc cggaacagca tcgtgagcag cgtgtcgatg acgacgatcg
     1501 agaacagaca cgacaccacg gtgctggtcg tgccgcggcc gacgcccgac gcggcgccgc
     1561 gggccgccag gccgtgctgg cagccgatca gcccgatcgc ggcaccgaac gccatgctct
     1621 tgcacagccc ggtccacacg tccgaggcct ccaccgcggt gcgcagctcg gcgaggaacc
     1681 ccgccggcgt gatatccagg ctggcctgac cgacgatgat gccgccgatc accccgacca
     1741 cgtcgccgag caaggtcagg accggcgcca ccaccacgag cgcgaggacc cgcggcacga
     1801 ccaggtacgg caccggcgcg aagcccatcg tgcgcagcgc gtcgatctcg tcggacaccc
     1861 gcatcgttcc gagctcggcg gcgtacgcgg cgcccgaccg cccgacgatg atgatcgcgg
     1921 tcatcagcgg cgcgagctcg cgcggcaccg acaggccgac gaggtcggcg gcgtagatgt
     1981 tggcgccgaa cagcgcgagc tggcgcgccg cctggtaggc catcacgaag ccgatcagga
     2041 agttgagcac caggacgatc gggatcccgt cggtgccggc ctggacgatc aggccgggca
     2101 ggtcgtgcca gctcaccttc gccggccggc gcacggccgc ggcgaccgcg gcgatcagct
     2161 cgccgaggaa cgcgaccatc tcgcgggcct gccagagcgc gtgctcgacc gcgccgccca
     2221 gccgcgcgat gccgcgctca cggcgccgga cctcggccac ccggggccgc tcgtcgccgc
     2281 ggtagaggtg gaccagcgcg cgctgcgatt ccgagccggt ggtgatcaag cagcgcacgc
     2341 cgcgcg
//
LOCUS       DASVOR010002299         2106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_259311,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002299 DASVOR010000000
VERSION     DASVOR010002299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259311"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            186..590
                     /locus_tag="VF516_42455"
     CDS             186..590
                     /locus_tag="VF516_42455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114488.1"
                     /translation="MSLRSFMSTSASTSCGVSGGADRQGAERTDLRFRERDDRIDEAR
                     AGHEDAAAEGVPDDDEGLRDGGPREELADLKERLRTVLATLDPLRLLEEIRIVRQHLA
                     GLAKGEVQHVLPDRDADLDRSLRSLAIAWKDR"
     gene            631..1953
                     /locus_tag="VF516_42460"
     CDS             631..1953
                     /locus_tag="VF516_42460"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8114489.1"
                     /translation="MSPLANQRDDAYGGSLAHRMRFPLEVFEAVRAAFPEDRPVGVRV
                     SATDWMDHGWDLEQTIALAREPAARGADWIDVSSGGISPAQKIALGPGYQVLFAEVVK
                     RATGLPTRAVGLITTPAQAEGIVANGKANLVALARAMLYDPRWPWHAAAEFGATVDAL
                     PQYWRSAPAGHRGCSGTRRRVDGGPSEACMTGHGRVAIITGAGRGIGKATALAFLRDG
                     YRVALAGRHRDTLDATAAESRAGDRALVIPADVTDPGAVDALFDAVRQAFGRLDVLFN
                     NAGTGARPVSIDELTVAEWRRVVDTNLTGTFLCARAAFRLMKDQDPRGGRIINNGSIS
                     AHAPRPRSAPYTATKHAITGLTKSLSLDGRPHDIACGQIDIGNALTEMTAPMASGVPQ
                     ADGSVRPEPTIDVAHIANAVLHMANLPLDANVQFMTIMATKMPFVGRG"
BASE COUNT          301 a          745 c          767 g          293 t
ORIGIN      
        1 gaccgcccgc cgcgctgggc gatcgggcgc ggagtcggca gcgatcagcg agctagacgc
       61 agaggacgcc acgcgggctc gcgggtggcg tcctcgtctg ggcgcgcaga aagcggacga
      121 gatagtccct cagggtcgcc ggctcgacga tgttgtcgag gaggccgcgc tgcaggagtc
      181 tccgcatgtc gctcaggtcc ttcatgtcga cgagcgcctc gacctcgtgc ggagtctcgg
      241 ggggggcgga tcgtcagggc gccgaacgga ccgatcttcg cttccgggaa cgcgacgacc
      301 gtatcgacga ggcccgggcc ggccatgaag acgccgccgc cgaaggcgtt ccggacgatg
      361 acgagggcct tcgggacggt ggacctcgcg aggagcttgc tgacctgaag gagaggctac
      421 gtaccgtcct cgcgactctc gatccgctcc gcctgctcga agagatccgc atcgtccggc
      481 aacatctcgc agggctcgcc aagggtgagg tccagcatgt tcttcctgat cgcgatgccg
      541 acctcgaccg ctccttgcgc agcctcgcga ttgcgtggaa agaccggtag gtacgcccac
      601 tcgtgtgacc ggccccctgt gccagggtag ttgtcgccgc tcgccaacca gcgcgacgac
      661 gcctacggcg gctcgcttgc ccaccgcatg cggttcccgc tggaggtgtt cgaggcggtc
      721 cgcgcggcgt tcccggagga ccgcccggtc ggcgtccggg tctcggcgac cgactggatg
      781 gaccacggct gggacctgga gcagacgatc gcgctcgccc gcgagcccgc cgcgcgcggc
      841 gccgactgga tcgacgtgtc gtcgggcggc atctcgccgg cccagaagat cgcgctgggg
      901 cccggctacc aggtgctgtt cgccgaggta gtcaagcgtg cgaccgggct gccgacgagg
      961 gcggtcggcc tgatcaccac gccggcgcag gccgagggca tcgtcgcgaa cggcaaggcc
     1021 aacctggtcg cgctcgcccg cgcgatgctc tacgatccgc gctggccgtg gcacgcggcc
     1081 gccgagttcg gcgccacggt cgacgcgctg ccgcagtact ggcggtccgc gccggctggc
     1141 caccgtggct gttcggggac cagacgccgc gttgacggcg gaccctcgga ggcatgcatg
     1201 accggtcacg gcagggttgc gatcatcacc ggcgcggggc gcggcatcgg caaggccacc
     1261 gcgctcgcgt tcctgcgcga cggctaccgc gtcgcgctcg cggggcgcca ccgcgacacg
     1321 ctcgacgcca ccgccgcgga gtccagggcg ggcgaccgcg cgctcgtgat cccggccgac
     1381 gtgacggatc ccggtgcggt cgacgcgctg ttcgacgccg tccgccaggc gttcggccgg
     1441 ctcgacgtgc tgttcaacaa cgcggggacc ggcgcccggc cggtgtcgat cgacgagctc
     1501 accgtcgccg agtggcgccg tgtcgtcgac accaacctga ccggcacgtt cctgtgcgcg
     1561 cgggcggcgt tccggctgat gaaggaccag gacccgcgcg gcgggcgcat catcaacaac
     1621 ggctcgattt cggcgcacgc gccgcggccg cgctcggcgc cctacaccgc gaccaagcac
     1681 gcgatcactg ggctcacgaa gtcgctgtcg ctcgacggcc ggccgcacga catcgcgtgc
     1741 ggtcagatcg acatcggcaa tgcgctgacc gagatgacgg cgccgatggc cagcggcgtc
     1801 ccgcaggccg acggctcggt gcggccggag ccgacgatcg acgtcgcgca catcgcgaac
     1861 gccgtgctgc acatggccaa cctgccgctt gacgccaacg tgcagttcat gacgatcatg
     1921 gcgaccaaga tgccgttcgt cggacgcggc tgagcgaggg ccaggcatga cgcggagctg
     1981 gaaagggtac gcggcgctcg tgatcgcgtg cgcggcgctc gcggtcgcgg ttcacgccga
     2041 gctgcgcggt ggcgcgtctc cgcccggccc cgcggcgtgc gtcgatcaga ccgcgcgcga
     2101 ccaggt
//
LOCUS       DASVOR010002300         4663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_463203,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002300 DASVOR010000000
VERSION     DASVOR010002300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463203"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1025)
                     /gene="amrS"
                     /locus_tag="VF516_42465"
     CDS             complement(<1..1025)
                     /gene="amrS"
                     /locus_tag="VF516_42465"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEX8114490.1"
                     /translation="MDDKRKSLPGILARSSAPARLWEPLDGGRVQCRLSPRECKIPPG
                     GLGFCGVRYNDGGALVTLNYGKSVPMTQETIETEAVYHYAPGARILSMGNIGCMMKCD
                     FCHNWQTSQARLARDQDIVAYSPEEVVEYALRHGIGVLSWTYNDPVVWHEFVVDTARL
                     GRKHGLVNLYKSAFYITPRAVDELLEVIDIFSLSLKSMNEDHYRKHTGGTLQPVLDAI
                     LQVYRANGPHLEISNLCVTGRNDNLDESMKVARWMLDHLDADVPLHYVRFHPDYQYTD
                     VARTSVPFLEQARRAAIDAGMRYVYLGNVFDTESVNTWCAGCGELLVRRYGLTAAVNL
                     RDGACPR"
     gene            complement(1165..2601)
                     /locus_tag="VF516_42470"
     CDS             complement(1165..2601)
                     /locus_tag="VF516_42470"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEX8114491.1"
                     /translation="MASAHDLRRAIAAVLAVALTAALGWLGTGLHPLWPLTWFASLPV
                     LMFVAGASWWRAALAAAIAWLGGMLGLWHHLHDVLQVPGVVIAQFYGVETIVFTLGVL
                     LYRALLRRGTYGWAVVAFPAVRVAFEYLQALGGPHGTFGSLAYTQVEFLPVLQLASVT
                     GPWGITFLVLLLPAAFATAWQLRSARRRALWIAGAAVAAVTAVLALGVDRMVGDESGP
                     LVRVGLVASDPPTSPRIADEGAATTALLDAYARSAEALAAQSAQLIVLPEKLGVVVDP
                     GTRDADARFQALADRTRAVIAVGLVHVAGAASYNEARVYAPDAPVQVYTKHHLLPPFE
                     PFQPGTALSLLPQPLGTWGVQICKDMDFPPLSRDYGRAGAALLLVPAWDFGADGFLHA
                     RMAVMRGVESGFSVVRAAKQGYLTVSDNKGRILAEAPSDAAPFATLVARVPAVHVETL
                     YQRFGDWFAWLVLAGLAVAIRQIFRRRH"
     gene            complement(2585..3166)
                     /locus_tag="VF516_42475"
     CDS             complement(2585..3166)
                     /locus_tag="VF516_42475"
                     /inference="COORDINATES: protein motif:HMM:NF024701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEX8114492.1"
                     /translation="MARAPTADRIIAAARRLLEREGTEAVTMRRVGDAVGITAMAIYR
                     HYPDRTALLNAVADQGFAELAAAVQRMRLPAGLEPRLLRMVDVYVDHALAHPRLFELM
                     FLAPRKGARRYPRDFKAGRSPTINPSVAILQEGMARGELRADDPFEIAFALGALLEGL
                     VMLYLGGRIGGGPRQLRALIRRSIRRHLHGICT"
     gene            complement(3316..3861)
                     /locus_tag="VF516_42480"
     CDS             complement(3316..3861)
                     /locus_tag="VF516_42480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8114493.1"
                     /translation="MQSPRDQIIETLDALAAPLGPQTSVDERLERQDRWVDEGGVELL
                     EALLGVVASPPPEEQLQYASLDDWKTLLIEVAGALGKRHPDVALQRLLPLLDDDHARA
                     AAIDILGSVGDARAVPRLGQLMQGRRLGEDDLVRLAGALGEIGGDEACRLLAQLRQAV
                     MPAQQELRREIDIAVQAARCG"
     gene            complement(4096..>4663)
                     /locus_tag="VF516_42485"
     CDS             complement(4096..>4663)
                     /locus_tag="VF516_42485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114494.1"
                     /translation="SLQRDLRDTRAKLAQITLERDRLVSELRDARGDDADTNQRAAVK
                     TGFDPDATAQLDLAKLAKYEALAARSSELEQKVAQLEQGDAALRRQLADAEERLRLAA
                     IDLEDDSELTRTGNQLPISELAEHVNVLEESIDSLRANMRAASDETAMMDPSESVNTI
                     SHAVSQAAEHVERARAAIRALSASIGVS"
BASE COUNT          720 a         1604 c         1659 g          680 t
ORIGIN      
        1 cagcgcgggc aggcgccgtc gcgcaggttc accgccgcgg tcaggccgta gcggcgcacg
       61 agcagctcgc cgcagccggc gcaccacgtg ttgacgctct cggtgtcgaa cacgttgccg
      121 aggtagacgt agcgcatgcc ggcgtcgatc gccgcgcggc gcgcctgctc caggaatggc
      181 accgaggtcc gcgcgacgtc cgtgtactga tagtcggggt ggaaccgcac gtagtggagc
      241 ggcacgtcgg cgtcgaggtg gtcgagcatc cagcgcgcca ccttcatgct ctcgtcgagg
      301 ttgtcgttgc gcccggtgac gcacaaattc gagatctcga ggtgcgggcc gttcgcccgg
      361 tagacctgca ggatcgcgtc gagcacgggc tgcagcgtcc cgccggtgtg cttgcggtag
      421 tggtcctcgt tcatcgactt gagcgacagg ctgaagatgt cgatcacctc gagcagctcg
      481 tcgaccgcgc gcggcgtgat gtagaacgcc gacttgtaca ggttgacgag cccgtgcttg
      541 cgtccgagcc gcgccgtatc gacgacgaac tcgtgccaca cgaccggatc gttgtaggtc
      601 cacgacagca caccgatgcc gtggcgcagc gcgtactcga cgacctcctc gggactgtac
      661 gcgacgatgt cctgatcgcg tgcgagccgc gcctggctgg tctgccagtt atggcagaag
      721 tcgcacttca tcatgcagcc gatgttgccc atcgagagga tgcgcgcgcc gggtgcgtag
      781 tgatagaccg cctcggtctc gatcgtctcc tgcgtcatcg ggaccgactt gccgtagttc
      841 agcgtcacga gcgcgccgcc gtcgttgtag cggacgccgc agaacccgag cccgcccggt
      901 gggatcttgc actcgcgcgg cgacaggcgg cactgcacgc gcccgccgtc gagcggctcc
      961 cacaggcgcg ccggcgccga cgatcgggcg aggatgccgg gcagggactt tcgtttgtcg
     1021 tccatcggga cctccgctcc atcatagaga gaaccacgcc ggctggcgcg atccaccgcg
     1081 cgagcgagga cttttgacgc accgcttcgg ggatcgcccc gctacctcgg cgcatcccca
     1141 gctgctgggg tacggcgcct caggtcagtg ccgtcgccgg aagatctgcc tgatcgcgac
     1201 cgcgaggccg gccagcacga gccaggcgaa ccagtcgccg aatcgctggt agagcgtctc
     1261 gacgtgcacc gcggggacgc gagccacgag tgtcgcgaac ggcgcggcgt cgctgggcgc
     1321 ctcggcgagg atgcggccct tgttgtcgct gaccgtgaga tagccctgct tggcggcgcg
     1381 caccacgctg aagccgctct ccacgccgcg catcacggcc atgcgggcgt gcaagaagcc
     1441 atcggcgccg aagtcccagg ccgggaccag caagagcgcc gcaccggcgc ggccgtagtc
     1501 gcgcgacagc ggtgggaagt ccatgtcctt gcagatctgg acgccccatg tgccgagcgg
     1561 ctgtggcagg agcgacagcg ccgtcccggg ttggaatggc tcgaacggcg ggagcaggtg
     1621 gtgcttggtg tagacctgta ccggggcgtc cggtgcgtag acccgcgcct cgttgtacga
     1681 cgccgcgccg gccacgtgga ccagacccac ggcgatcacg gcgcgggtcc ggtcggccag
     1741 cgcctggaac cttgcgtcgg cgtcgcgagt cccgggatcc acgaccacgc cgagcttctc
     1801 gggcagcacg atcagctgcg cgctttgggc ggcgagcgct tcggcgctgc gcgcgtacgc
     1861 gtcgagcagc gcggtggtgg cggcgccctc gtcggcgatc cgagggctgg tcggcggatc
     1921 ggaggcaacg aggccgacgc ggaccagcgg gcccgactcg tcgccgacca tccgatcgac
     1981 gcccaacgcg agcaccgccg tgacggcggc gaccgccgcg ccggcgatcc acagcgcccg
     2041 ccgccgcgcg cttcgcaact gccacgcggt ggcgaatgcc gccggcagca gcaggaccag
     2101 aaacgtgatc ccccacggcc cggtgaccga cgccagctgc agcaccggca ggaactcgac
     2161 ctgcgtgtag gcgaggcttc cgaacgtgcc gtgtggcccg ccgagcgcct gcaggtactc
     2221 gaatgcgacc cggacggcag ggaatgcgac gacggcccag ccgtacgtgc cacggcggag
     2281 cagcgcgcga tacagcagca cgccgagcgt gaacacgatg gtctcgaccc cgtagaactg
     2341 cgcgatgacc acgcccggga cctgcaggac atcgtgcagg tggtgccaga ggccgagcat
     2401 gccgcccagc cacgcgatcg cggcggcgag cgccgcgcgc caccaggacg ccccggccac
     2461 gaacatcagc acaggaagcg aagcgaacca ggtcagcggc cacagcggat gcagcccggt
     2521 gcccagccag cccagcgccg cggtcagcgc cacggcgagc acggcggcga tggcgcgccg
     2581 cagatcatgt gcagatgcca tgcagatgcc tccggatgct gcgccggatc agggcgcgga
     2641 gttgacgtgg gccgccaccg atgcgcccgc cgagatagag catcaccagg ccctccagca
     2701 gcgcaccgag tgcgaacgcg atctcaaagg ggtcgtcggc ccgcagctcc ccgcgggcca
     2761 tgccttcttg cagtatggcg acgctcgggt tgatggtcgg cgagcgcccg gccttgaagt
     2821 cgcgtggata gcggcgagcg cccttgcggg gcgcgaggaa catgagctcg aacagccgcg
     2881 ggtgggcgag cgcgtggtcg acgtacacat cgaccatccg cagcagccgg ggctcgagcc
     2941 ccgccggcag ccgcatgcgc tgcaccgccg cagcgagctc ggcgaacccc tggtccgcca
     3001 ccgcgttgag cagtgctgtg cgatccgggt agtggcggta gatcgccatc gcggtgatgc
     3061 cgaccgcatc acccacccgg cgcatcgtca cagcttcagt accctcgcgt tccagcagcc
     3121 ggcgcgccgc ggcgatgatc cgatctgcgg tgggggcgcg cgccatgtat acactgtata
     3181 catgatgtcc gcgatccgca caagcagcga cgtctggcag gatcaggcat gtgcagcgcc
     3241 gaggaggtgc ggtggagtcc tggccatggc gcgcgtggat cgccaacgcg agcgccaggc
     3301 gatgcggggg ggcactcaac cgcagcgggc ggcttgcacc gcgatgtcga tctcgcgtcg
     3361 tagctcctgc tgcgcgggca tcacagcttg ccgcagctgc gcgagtaggc gacatgcttc
     3421 gtcaccaccg atctcgccca gcgcgccggc aaggcgaacc agatcgtcct cgccgaggcg
     3481 acggccctgc atcaactgac cgaggcgagg cacagcgcgc gcatcaccga cactgccgag
     3541 gatgtcgatg gcggcggccc tcgcgtggtc atcgtcgagc agaggcaaga ggcgctgcaa
     3601 cgccacgtcg gggtgccgct tgccgagtgc accggccacc tcgatcagga gcgttttcca
     3661 gtcgtcgagg ctggcgtact gcagctgttc ttctggagga ggcgatgcga ccaccccgag
     3721 caacgcctcg agcaactcga cgccaccttc gtcaacccag cggtcctgtc gttcaaggcg
     3781 ctcgtcaacg ctcgtctggg gcccaagagg cgccgccagg gcatcgagcg tctcgatgat
     3841 ctgatctcgg gggctctgca tttctagttc tccaccacca gctcgtagat cgttctcgtc
     3901 gtctctgatc cagcccagta tcccacgccg cctgcgatca gggtccccac accgtgggca
     3961 gccaggccca gccgactccc gcgaggagga gcccccgacg gcgccgcgag cgagcgtgta
     4021 cgtggtctcc caggagatga cgagcaggcg ggagagtgcg cgatctggtc gacgtacgcg
     4081 cggcggcgcg gcgggtcagc tcacgccgat cgaggcggac agcgcgcgga tcgcggcgcg
     4141 ggcgcgctcg acgtgctcgg cggcctggct gaccgcgtgc gagatggtgt tgaccgactc
     4201 ggaggggtcc atcatcgcgg tctcgtcgct cgcggcccgc atgttggcgc gcagcgagtc
     4261 gatcgactcc tcgaggacgt tgacgtgctc ggcgagctcg ctgatcggca gctggttgcc
     4321 ggtgcgggtc agctcgctgt cgtcctcgag gtcgatcgcg gcgagccgca gccgctcctc
     4381 ggcgtcggcg agctggcggc gcagcgcggc gtcgccctgc tcgagctgcg cgaccttctg
     4441 ctcgagctcg ctggaccggg cggcgagcgc ctcgtacttg gcgagcttgg cgaggtcgag
     4501 ctgggcggtc gcgtcggggt cgaacccggt cttgaccgcg gcgcgctggt tggtgtcggc
     4561 gtcgtcgccg cgcgcgtcgc gcagctcgct gaccaggcgg tcgcgctcca gcgtgatctg
     4621 cgcgagcttg gcgcgggtgt cgcgcaggtc gcgctgcagc gac
//
LOCUS       DASVOR010002301         1917 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_54934,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002301 DASVOR010000000
VERSION     DASVOR010002301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54934"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..566
                     /locus_tag="VF516_42490"
     CDS             <1..566
                     /locus_tag="VF516_42490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8114495.1"
                     /translation="FPEELRDRSHLHWTPVAVAIRAAELLAPSPHGIRVLDVGAGVGK
                     LCLIGALVTGAVWWGIEQDAVQISAASHAAWALDVTRRTRFVQGDGSRLSWDGFDAFY
                     FYNPFHTVMLAPHASPFVRYATIQGTLRRIEQRLAATPPGTRVVTFHGFGGPLPASFT
                     RIAREPVGDGALELWIQGAPSACSGAL"
     gene            complement(720..1676)
                     /locus_tag="VF516_42495"
     CDS             complement(720..1676)
                     /locus_tag="VF516_42495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114496.1"
                     /translation="MKAVLVCLGIALAFGACKKSGGTGPTGTGGGGGSSWLVGTGGLM
                     VNVNTNGYSQGYELSSPETLNKIACRYAGEAWVVGDHGTLLYTDDAGATWRPQPVPTT
                     ANLRGIATQDAGPVFVAGDGVLLTSTDTGQHWAPLGDGRTSFVAVAAAQGAENALAVS
                     DTGAVFAIENHQLVSRGSFTGARAVAISPDGDSAIVVGDQLITRSSDGGRTWTPLAVG
                     GNVRFDDVRIDDTGEALAVGSSGTIAHITAGGAVTLQQVGPADLHAVHIADSDDGAEA
                     VGFAAGDSGRVFITRDGGTTWSPGPTVNGTVLGLDMIGAGHL"
BASE COUNT          244 a          696 c          694 g          283 t
ORIGIN      
        1 tgttccccga ggagctgcgc gaccgctcgc acctgcactg gaccccggtc gcggtcgcga
       61 tccgcgccgc cgagctgctc gcgccgtcgc cgcacgggat ccgcgtgctc gacgtcggcg
      121 ccggggtcgg caagctgtgc ctgatcggcg cgctggtcac cggcgcggtg tggtggggaa
      181 tcgagcagga cgcggtgcag atctcggccg ccagccatgc ggcgtgggcg ctcgacgtca
      241 cccggcggac ccggttcgtg cagggcgatg gctcgcggct gtcgtgggat gggttcgacg
      301 cgttctactt ctacaacccg ttccacaccg tgatgctcgc gccgcacgcc agcccgttcg
      361 tgcgctacgc gacgatccag ggcacgctgc ggcggatcga gcagcggctc gccgcgacgc
      421 cgcccggcac gcgggtcgtc acgttccacg gcttcggcgg cccgctcccg gccagcttca
      481 cgcggatcgc gcgcgagccg gtcggcgacg gtgcgctcga gctgtggatc cagggcgcgc
      541 cgagcgcctg ctcgggcgcc ctgtagtcgt gcggggcgag aaccccgggg ctgcagctgc
      601 tgatgcgacg cgcagggcgc gaagtcgatc tcgcgccatg caacgccaca aacggccgcc
      661 ggtcaaagcg gccgttcccc aggattgcca gcagctgcag cccggagagg gtgggccggc
      721 tacaggtggc cggcgccgat catgtcgagg ccgagcacgg tcccgttgac ggtgggaccc
      781 gggctccagg tggtgccgcc gtcgcgcgtg atgaacaccc ggccgctgtc gcccgccgcg
      841 aacccgacgg cctcggcgcc gtcatcgctg tccgcgatgt ggaccgcgtg cagatccgcg
      901 gggccgacct gctgcagggt cacggcgccg ccggccgtga tgtgcgcgat cgtgccgctg
      961 ctgccgaccg ccagcgcctc gccggtgtcg tcgatccgga cgtcgtcgaa ccggacgttg
     1021 ccgccgaccg cgagcggcgt ccacgtgcgc ccgccgtcgc tgctccgtgt gatcagctgg
     1081 tcgccgacca cgatcgcgct gtcgccgtcg ggcgagatcg cgaccgcgcg ggcgccggtg
     1141 aagctgccgc ggctgaccag ctggtggttc tcgatcgcga acaccgcgcc ggtgtcggac
     1201 accgcgagcg cgttctcggc gccctgcgcc gccgcgacgg cgacgaagct ggtgcggcca
     1261 tcgccgagcg gcgcccagtg ctgcccggtg tcggtcgagg tcaggagcac gccgtctccg
     1321 gcgacgaaca ccggcccggc gtcctgcgtt gcgatcccgc gcaggttcgc ggtggtcggc
     1381 accggctgcg gccgccacgt cgcgccggca tcgtcggtgt agagcagcgt gccgtggtcg
     1441 ccgacgaccc aggcctcgcc tgcgtaccgg cacgcgatct tgttgagcgt ctccggggag
     1501 gacagctcgt agccctgcga gtagccgttc gtgttgacgt tgaccatcag cccgccggtg
     1561 ccgaccagcc agctggaacc accgccgccg ccggtgccgg tcgggccggt gccgccgctc
     1621 ttcttgcaag ctccgaaagc cagggcgatg cccaggcata cgagtactgc cttcataagt
     1681 cctcctaggg gggatacccg gtggttcgca cgcggatgct actctgcaga gggcgcgtgc
     1741 aacgcaacgt cgcacccgga cggccaatta attcttaact ctctagaatc aattcggaag
     1801 tagcgctccc accaggacgt cggcgcggtc gaccgcccgc tgggcgcgcc ggagcagctc
     1861 ggccggcgcg cgccggccct ggcgggctgc cgacaccgcg cggtcgatct cgcggat
//
LOCUS       DASVOR010002302         1577 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113743,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002302 DASVOR010000000
VERSION     DASVOR010002302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113743"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="VF516_42500"
     CDS             complement(<1..756)
                     /locus_tag="VF516_42500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114497.1"
                     /translation="MTMSDVQRTRLARPVRLLYWLTLIAGLALFTATRRGEPAVTAAL
                     WTGAIGGTLLGQYFALRNYRLWVAALAVAAVGLYCAPLVWIGEASTQLWLSFFPAALC
                     GFWSLGDRSGFAALWFPTVLWMLTILDRSDRIAPEGPAAILLGGLAALFIGFLWIRES
                     RRVALWRSVSAEPLADTLPTELLREPPGRALARVGWWLTVGALTAAITVWLVPPLWQS
                     ETLGGLPTQLAAATPTRIWPGLPCCPVHGDADPL"
     gene            complement(753..>1577)
                     /locus_tag="VF516_42505"
     CDS             complement(753..>1577)
                     /locus_tag="VF516_42505"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEX8114498.1"
                     /translation="RPAAVDGVRALLGAGGDDAIALPTQRLPTEGTFRIRNYLPGDDA
                     RRIHWVRSLQTNRLVVRLADEIPQAEPAVRLILDNELWGADTLACRQVRELLDTLVRV
                     WLGTARALTDAGTRVILVAIAERDGAPSVIERPFVPHATRDVLRLAGRITWQTALTLS
                     SLVAADASTRQVVVSSRSRKLVAASPLAWVVVPEVAWTSTAPPVPARSLARLPYPAGS
                     ADNRLGRRIRDREHHQLVHHDHVLLSQVMCRTELASFSGELIARPQRGRVALEVIP"
BASE COUNT          222 a          531 c          663 g          161 t
ORIGIN      
        1 cagggggtcg gcgtcgccgt ggaccgggca gcatggcagg ccgggccaga tccgggtggg
       61 cgtggcggcg gcgagctggg tcggcagacc gccgagggtc tcgctctgcc acagcggcgg
      121 gacgagccac acggtgatcg cggcggtgag cgcgccgacc gtgagccacc agccgacgcg
      181 ggcgagcgcg cggccgggcg gctcgcgcag gagctcggtg ggcagggtgt ccgcgagcgg
      241 ctcggcggag acgctgcgcc acagcgcgac gcggcgcgac tcgcggatcc acaggaagcc
      301 gatgaacagg gcggcgaggc cgccgagcag gatcgcggcg gggccctcgg gcgcgatccg
      361 gtcgctgcgg tcgaggatgg tgagcatcca gagcacggtg gggaaccaca gggcggcgaa
      421 cccgctgcga tcgcccaggc tccagaaccc gcacagcgcg gccgggaaga acgacagcca
      481 cagctgggtg gaggcctcgc cgatccatac cagcggtgcg cagtacaggc cgaccgcggc
      541 gacggcgagc gccgcgaccc acaggcggta gttgcggagc gcgaagtact ggccgagcag
      601 cgtgccgccg atcgcgccgg tccacagcgc cgcggtcacg gcgggctcgc cgcggcgggt
      661 cgcggtgaac agggcgaggc cggcgatcag ggtcagccag tagagcaggc gcaccgggcg
      721 ggcgagccgg gtgcgctgga cgtcggacat ggtcatggga tcacctccag cgcgacccgg
      781 ccgcgctgcg ggcgcgcgat cagctcgccg gagaagctcg cgagctcggt ccggcacatc
      841 acctggctga gcaggacgtg gtcgtggtgc accagctggt ggtgctcgcg gtcgcggatc
      901 cggcggccga ggcggttgtc ggcgctgccg gcggggtagg gcagcctggc cagcgagcgg
      961 gccggcacgg gcggcgcggt ggaggtccac gcgacctccg gaacgacgac ccaggcgagc
     1021 ggggacgcgg cgaccagctt gcgcgaccgg ctcgacacca cgacctggcg ggtcgacgcg
     1081 tcggcggcga ccagcgagct cagggtcagc gcggtctgcc aggtgatccg gccggcgagc
     1141 cgcagcacgt cgcgggtcgc gtgggggacg aacggccgct cgatgaccga gggcgcgccg
     1201 tcgcgctcgg cgatcgcgac caggatgacg cgggtgccgg cgtcggtcag cgcgcgggcc
     1261 gtgccgagcc agacccggac cagggtgtcg agcagctcgc ggacctggcg gcacgccagg
     1321 gtgtcggcgc cccacagctc gttgtcgagg atcaggcgga cggcgggctc ggcctgcggg
     1381 atctcgtcgg cgagccgcac gacgagccgg ttggtctgca gcgagcgcac ccagtggatc
     1441 cgccgggcgt cgtcgccggg gaggtagttc cggatccgga atgtgccctc ggtcggcagg
     1501 cgctgggtcg gcagcgcgat cgcgtcgtcg ccgccggcgc cgagcagcgc gcgcacgccg
     1561 tcgaccgcgg ccggccg
//
LOCUS       DASVOR010002303         1521 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_26595,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002303 DASVOR010000000
VERSION     DASVOR010002303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26595"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /locus_tag="VF516_42510"
     CDS             <1..1076
                     /locus_tag="VF516_42510"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEX8114499.1"
                     /translation="VHAIGRAVDGERVTVSHACQAGRPLIDASLRDLRARWSHVSHQI
                     ARLRDDPTCADEEHAARLACLVPPGAPGLTAELTFPVDPSLGLLGPLGASPMVARGAR
                     PRVAILREQGVNGHIEMAAAFLRAGFEPIDVHMTDLVAGRADLSDCRGAVACGGFSFG
                     DVLGGGRGWAATFRYNPRARDALTRFTARSDTFLLGVCNGCQMLADLTDLLPGAAGWP
                     RFVRNRSEQFEARLVLLRIEPGPSILLRGMAGSRIPVVTAHGEGRAELDAAQLAALEG
                     AGAVAARFVDGGGAVAASYPANPNGSPGGIAALTNADGRITIMMPHPERVFRTAQLSW
                     HPPEWGEDSPWMRMFYNARAWVG"
     gene            complement(1117..>1521)
                     /locus_tag="VF516_42515"
     CDS             complement(1117..>1521)
                     /locus_tag="VF516_42515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114500.1"
                     /translation="RRDSWLAATAMVSALLALPLAQNAWHGPEVAAILAIAATAQFAG
                     HRWAIAVIVLSELLLLPTVWPRAFLDSGDLSSRLSALITLVAMVPGLLAMRRAAAALV
                     VLTGQRHPRQMRRILHAGLLITCAIAIAVPWL"
BASE COUNT          188 a          566 c          567 g          200 t
ORIGIN      
        1 aggtccacgc gatcggccgc gcggtcgacg gcgagcgcgt cacggtgtct catgcttgcc
       61 aggccggccg ccccctgatc gacgcgtcac tccgcgatct gcgcgcgcgg tggtcgcacg
      121 tcagccacca gatcgcccgg ctgcgcgatg acccgacctg cgcggacgag gagcacgcgg
      181 cgcggctggc ctgccttgtg cccccgggcg cgccggggct gaccgccgag ctcacgttcc
      241 cggtcgatcc ttcgcttggc ctgctcggcc cgctcggcgc atcgccgatg gtggcgcgcg
      301 gcgcgcggcc gcgggtcgcg atcctccgcg agcagggcgt caacggtcat atcgagatgg
      361 ccgccgcgtt cctgcgcgcc gggttcgagc cgatcgacgt ccacatgacc gacctcgtgg
      421 ccggccgcgc cgatctgtcc gattgccgcg gcgcggtggc gtgcggcggg ttctcgttcg
      481 gcgacgtgct cggcggcggc cgcggctggg cggcgacgtt ccgctacaac ccgcgcgcga
      541 gagacgcgct cacccggttc accgcgcgca gcgacacctt cctgctcggc gtgtgcaacg
      601 gctgccagat gctcgccgac ctcaccgacc tgttgcccgg cgcggccggc tggccgcggt
      661 tcgtgcgcaa ccgctccgag cagttcgagg cccgcctcgt gctgctgcgg atcgagcccg
      721 gcccgtcgat cctgctccgc ggcatggccg gctcgcggat cccggtcgtc accgcgcacg
      781 gcgagggccg ggcggagctc gacgccgcgc agctcgcggc gctggaaggt gccggcgcgg
      841 tcgccgcgcg gttcgtcgac ggcgggggcg cggtcgcggc gagctatccg gcgaacccga
      901 acggctcgcc cggcggcatt gccgcgctga ccaacgccga tggccggatc acgatcatga
      961 tgccgcaccc cgagcgggtg tttcgcacgg cccagctgtc gtggcacccg cccgagtggg
     1021 gcgaggacag cccgtggatg cgcatgttct acaacgcgcg cgcctgggtc ggctgacgcg
     1081 tcgatgatgg caggcgccgg ccgcggtcgc gcccggtcac agccagggta cggcgatcgc
     1141 gatcgcgcag gtgatcagca gcccggcgtg gaggatccgc cgcatctggc ggggatggcg
     1201 ctggccggtg agcacgacca gcgcggcggc ggcgcgccgc atcgcgagca gcccgggcac
     1261 catcgccacc agcgtgatca gcgccgacag ccgcgagctc aggtcgccgc tgtccaggaa
     1321 tgcccgcggc cacaccgtgg gcagcagcag gagctcggac agcacgatca cggcgatcgc
     1381 ccagcgatgg ccggcgaact gcgcggtcgc ggcgatcgcc aggatcgcgg cgacctccgg
     1441 tccatgccag gcgttctgcg cgagcggcag cgcgagcagc gcactgacca tcgcggtcgc
     1501 ggcgagccac gaatctcggc g
//
LOCUS       DASVOR010002304         4544 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_521438,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002304 DASVOR010000000
VERSION     DASVOR010002304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521438"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..965)
                     /locus_tag="VF516_42520"
     CDS             complement(<1..965)
                     /locus_tag="VF516_42520"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8114501.1"
                     /translation="MAATASPPLVVEPQTILDLLPHGVVVVGRDWRITYANPEAERML
                     GATGATLWERCPALEHAAFASGFRYAMADRTELLTESVLPSVGWCQARARPTPDGGLL
                     ISLRQVHAHTIETGQARAALLLGEIGDALTREDSLRAALRRCAEGMVRHLEASQARIW
                     TVDSTARMLHLCASAGIDTPLDEKFAHLRFDEHKIGAIADEGFPYLTNDVPIDVDVGN
                     TDWVRDDRLVAFAGYPLRIEDRIVGVMAMYSRRKLDHDTLNGLSAIADALALGIASKL
                     ADEARRAAEAALRRHAGDLEALHALGQQLTAVLEPEALAHKVIDAT"
     gene            1077..4523
                     /locus_tag="VF516_42525"
     CDS             1077..4523
                     /locus_tag="VF516_42525"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8114502.1"
                     /translation="MIALACDCARRRPDRRSPIADRRSLVEPRAAGSRRWKRFAISRL
                     RVSVGGREPSVIDLDDCPSENEIVGFLEGALSDPERARIEAHCANCERCREGVAQAAY
                     DSAAAVARPRDGAARPLDRELASVVQEVLGGSSSAEAAVGTWIGRFELVGVLGRGQFG
                     VVYRARDTQLGRMVAVKVMRRAGRQDRPIRETLFQTEAAAAARLQHPNIVTLHDYGEF
                     EGAPYLILELLEGDTLRTRLAGAGRLAPLDALAIASDVARALVEAHAAGVIHRDIKPG
                     NVFLCASGQTKVLDFGLAQLQSEVDRGSEPAGSAAARAGTPRYMAPELLRGQAADACT
                     DVYAVGLTLFEMLVGLLPSGGGPSIAERIAGAPLLPRLRRLLGKATAFERAGRFQHAG
                     ELLSALIRARSQLARRRHAGRRLGIPLAGVLLAATAAVAYRGLASSQRAGKPTVAVLG
                     FDDLSGRPDTAWLSTALSEMLDADLAASPAMHVVPPELVSHARVELGMKERDPVEPRT
                     LQDLRRYLGADFVVIGSYLSVAADAGTQTRLDVRIEDTRTQRRESFSETGRDNELLDL
                     VARIASRMRVRLAGTAIAAEEARTLRASMPAATEAVRLYAEGLRRWRSADLLEARDVL
                     ARAVAAEPDFPLAHSAFSQVLHALGEEARMRDEAQRAFELSSGLRRQERWLVEARYRE
                     AIGQWPQARELRRTLHELFPDDLEYGLELADALNCHRQNDEARAVIAELRRLPPPQRD
                     DPRIDLYDASAQTGYRVREALAISAADKAKALGVRWILGKARLQEADAERHLGELERS
                     LVAAEEARDIYQSIGDREGLSIALYEVASTRGMLGEAFSVELAGYEEALELERQIGNN
                     DQVTNMLGNIAFESLLAGYPAQAAARIDEIERLGTGTAPDQTERLRALQWLEQGDLAG
                     AFAQLEHVRRAFFEAGKSRGYELKLLGDVLMARGELDQARARVEQYLAQGWSAESGFH
                     VVGVLALSRFDLEAGQTQRAEARVREMIALCEAKHNISDLVTAQVLLARISIAEHRFE
                     EAKAAAEAALALAARTEAMPGWIDAQIALARAVLGLHPRDIDTALAAAQTALDRALER
                     GLLGHAYEARLAAGEIAAQGHRPDARAQLRALADEADQHGFGLIARKARAAAAAPQLT
                     ER"
BASE COUNT          651 a         1559 c         1672 g          662 t
ORIGIN      
        1 gtcgcgtcga tcaccttgtg agccagcgcc tcgggctcga gcaccgccgt cagctgctga
       61 ccgagcgcgt gcagcgcctc gaggtcaccg gcgtgccgcc gcagtgcagc ctcggcggcg
      121 cggcgggcct cgtcggccag cttgctggcg atgccgagcg cgagggcatc ggcgatcgcc
      181 gacaggccgt tgagcgtgtc gtgatcgagc ttgcgccgcg aatacatcgc catcacgccg
      241 acgatgcgat cctcgatccg caacggatag ccggcgaacg cgacgagccg atcgtcgcgc
      301 acccagtcgg tgttgccgac gtcgacgtcg atcggcacgt cgttggtcag gtacggaaat
      361 ccttcgtcgg cgatcgcgcc gatcttgtgc tcgtcgaacc gcaggtgggc gaacttctcg
      421 tcgagcggtg tgtcgatccc ggcgctcgcg cacaggtgca gcatccgcgc ggtgctgtcg
      481 acggtccaga tccgggcctg gctcgcctcg aggtggcgca ccatgccctc ggcgcagcgc
      541 cggagcgcgg cgcgcagcga gtcctcgcgg gtcagcgcgt cgccgatctc gccgagcagc
      601 agcgcggcgc gggcctggcc ggtctcgatc gtgtgggcgt ggacctggcg caggctgatc
      661 agcaggccgc cgtccggcgt cggtcgcgcg cgcgcctggc accagccgac cgacggcagc
      721 acgctctcgg tcaacagctc ggtgcgatcg gccatcgcgt agcggaaccc ggaggcgaac
      781 gccgcgtgct ccagcgccgg gcagcgctcc cacagcgtgg cgccggtcgc gccgagcatg
      841 cgctcggcct cggggttggc gtacgtgatc cgccagtcgc ggccgaccac gacgacgccg
      901 tgcggcagca ggtcgaggat cgtctgcggt tccaccacca gcggaggaga agcagtcgcg
      961 gccacgaacg cacgatacga gaccgtctcg aaggcgcacg gccggagtac gtgattcgaa
     1021 cgtgagatca cttgcgctga gcgcgctcgc actgtttgcc gagcgcgctc gccacagtga
     1081 tcgcgctcgc gtgcgattgc gctcgtcgaa gacccgatcg ccgatcgccg atcgccgatc
     1141 gccgatcgct cgtcgagccg cgcgcggccg ggtcgcgccg gtggaagcgc tttgcaatca
     1201 gcagattacg ggttagcgtc ggcggccggg agcccagcgt gatcgatctc gacgattgtc
     1261 ccagcgagaa cgagatcgtc ggcttcctgg agggcgcgct ctcggatccg gaacgggcgc
     1321 ggatcgaggc gcactgtgca aattgtgaac gctgccggga gggagtcgcg caggcggcgt
     1381 acgactcggc ggctgcggtg gcgaggccgc gggatggcgc cgcgcgtccg ctggaccgcg
     1441 agcttgcctc cgtcgtgcag gaagtcctgg gtggctcctc ctccgcggaa gccgccgtgg
     1501 gcacgtggat cggccggttc gagctcgttg gcgttctggg gcgcggccag ttcggcgtcg
     1561 tgtaccgtgc ccgcgatacc cagctcggcc gcatggtggc ggtgaaggtg atgcggcggg
     1621 ccggacggca ggatcggccg atccgcgaga cgctgtttca gaccgaagca gccgctgctg
     1681 cgcggctgca gcatcccaac atcgtcacgc tgcacgacta cggcgaattc gaaggagctc
     1741 cgtatctcat cctcgagctc ctcgaaggcg atacgctccg gacccggctg gcaggcgccg
     1801 gccgcctcgc gcctctcgac gcgctcgcga tcgccagcga tgtggcgcgc gccctggtgg
     1861 aggcgcacgc ggccggggtc atccatcgcg acatcaagcc cggcaacgtg ttcctgtgcg
     1921 ccagcggcca gaccaaggtg ctcgacttcg ggctggcgca gctgcagagc gaggtcgacc
     1981 ggggttccga gccggcgggc tccgccgctg cgcgggccgg gacgccacgg tacatggcgc
     2041 ccgagctgct gcgcggccag gcggcggatg catgcaccga cgtgtacgct gtcgggctga
     2101 cgctgttcga gatgctcgtc gggctgttgc cgtccggcgg cgggccctcg atcgcggagc
     2161 gcatcgccgg agcgcctctc ttgcctcgac ttcgtcgcct gctggggaag gcgaccgcgt
     2221 tcgagcgcgc cggccggttc cagcatgccg gtgagctcct gtcggcgctg atccgcgcac
     2281 gcagccagct cgcccggcgc cggcacgccg gccggcgcct gggtatcccg ctcgcgggcg
     2341 tgctgctcgc ggcgaccgcg gccgtggcgt atcgcggcct cgcatcctcg cagcgcgccg
     2401 gcaagcccac cgtcgccgtc ctcggcttcg acgacctgtc tggccgcccc gacaccgcgt
     2461 ggctgtcgac cgcgctgtcc gagatgctcg atgccgatct ggccgcgtcg ccggcgatgc
     2521 acgtcgtgcc acccgagctg gtgtcgcacg cgcgcgtcga gctggggatg aaggaacgcg
     2581 atccggtgga gccgcggacg ctgcaggacc tgcgcaggta tctcggagca gatttcgtcg
     2641 tgatcggttc gtatctgtcg gtcgcggcgg atgcgggaac ccagacccgc ctcgacgttc
     2701 gaatcgagga cacgcgaacg cagcggcgcg agtcgttcag cgagaccggc cgcgacaacg
     2761 agctcctcga tctggtggcg cggatcgcca gccgcatgcg agtccgtctg gccggcacgg
     2821 ccatcgcagc ggaagaggcc cgcacgctgc gcgcctcgat gccggcggcg accgaggcgg
     2881 ttcgcctcta cgccgagggg ttgcggcgat ggcgcagcgc ggatctgctc gaagcgcgcg
     2941 atgtcctggc ccgcgccgtt gccgctgagc ccgacttccc gctggcgcac tcggcgttct
     3001 cgcaggtgct ccacgccctc ggcgaggagg cgcggatgcg cgacgaggcc cagcgcgcct
     3061 tcgagctgtc cagcggtctg cgccgacagg agcgctggct cgtcgaggca cgatatcgcg
     3121 aggcgatcgg ccagtggccg caggcgcgcg agctgcgccg cacgctccac gagctgttcc
     3181 ccgatgacct cgagtacggg ctcgagctcg ccgacgccct gaactgccat cgccagaacg
     3241 acgaagcgcg cgcggtcatc gccgagctgc gacgcctccc accgccgcag cgcgatgatc
     3301 cacggatcga tctatacgat gcctcggcgc agacgggcta ccgcgtccga gaggcgctcg
     3361 ccatctcggc tgccgacaag gcgaaggcgc tcggcgtccg gtggatcctc ggcaaggcgc
     3421 gcctccagga agccgacgcg gagcggcacc tcggtgagct cgaacgctcg ctggtcgccg
     3481 ccgaggaggc gcgagacatc taccagtcga tcggcgatcg cgaggggctc tcgatcgcgc
     3541 tgtacgaggt cgcctcgacg cggggtatgc tcggcgaggc gttctccgtg gagcttgcgg
     3601 gatacgagga ggcgctggag ctggagcgac agatcggaaa caacgatcag gtcacgaaca
     3661 tgttgggcaa catcgccttc gaatccctgc tggcagggta cccggcgcag gcagccgccc
     3721 ggatcgacga gatcgaacgc ctggggaccg gtacggcgcc ggatcagacg gagcggttgc
     3781 gggccctgca gtggctggaa caaggcgatc ttgcgggagc gttcgcgcag ctcgagcatg
     3841 tccggcgcgc gttcttcgag gccggcaaga gccgcggtta cgagctgaag ctgctcggtg
     3901 acgtgctgat ggccaggggc gagctggatc aggcgcgcgc acgcgtggag cagtacctgg
     3961 cgcaaggctg gtcggccgag tccggatttc acgtggtcgg cgtgctcgcg ctgtcgcggt
     4021 tcgacctcga agcggggcag acgcagcgcg cggaagcgcg cgtgcgggag atgatcgccc
     4081 tgtgcgaggc aaagcacaac atctcggacc tcgtgaccgc ccaggtcttg ctcgcccgga
     4141 tctcgatcgc cgagcatcgc ttcgaggaag ccaaggcggc ggcggaggcc gcgttggcgc
     4201 tcgctgcgcg gaccgaggcg atgccgggct ggatcgatgc gcagatcgcg ctcgcgcggg
     4261 cggtcctcgg gttgcacccg cgcgacatcg acaccgctct ggcggcggcg cagacggcgc
     4321 tggatcgagc gctggagcgg ggcctgctcg gccatgccta cgaagcgcgg ctcgccgctg
     4381 gcgagatcgc cgcacaagga catcggccgg atgcgcgtgc gcagctgcgc gccctcgcgg
     4441 acgaggccga ccagcatggc ttcggcctga tcgcgcgcaa ggcacgggca gccgcagcgg
     4501 cgccgcagct caccgaacgc tgagcgcagg cgccgccccg gacg
//
LOCUS       DASVOR010002305         6127 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_171128,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002305 DASVOR010000000
VERSION     DASVOR010002305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6127)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6127)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6127
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171128"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..337
                     /locus_tag="VF516_42530"
     CDS             <1..337
                     /locus_tag="VF516_42530"
                     /inference="COORDINATES: protein motif:HMM:NF020419.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEX8114503.1"
                     /translation="ELDELLGTKLRALYQRKKGRDLFDLALALERQVASPDRIVEVFA
                     RYTHEGGARITRAMFEQNLAAKRADPIFTADLTPLLANGHAWSFDAAYDLVWRELISR
                     LPGEPWKGG"
     gene            complement(391..1014)
                     /locus_tag="VF516_42535"
     CDS             complement(391..1014)
                     /locus_tag="VF516_42535"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEX8114504.1"
                     /translation="MVAEPDCLQGDKLLVMGVPARQRFSFDEYLLLEEIAEVKHEFLD
                     GEAWAMAGGSPEHAAIIGNVTTLLNVQLRGQRCRVHSTELRVRVKATGLGTYPDVTVI
                     CGRLERDLDDRTGHTAINPRVIVEVLSPRTENYDRGEKLSHYQTIPSLEEIVLVAHDR
                     PEIEVVRREADGTWSRHIAGAGQSARLAAIACDLSVAEVYRDPFADA"
     gene            1272..1538
                     /locus_tag="VF516_42540"
     CDS             1272..1538
                     /locus_tag="VF516_42540"
                     /inference="COORDINATES: protein motif:HMM:NF039417.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6364 family protein"
                     /protein_id="HEX8114505.1"
                     /translation="MNLTLSLDERLVERAREVARQQGTSLNALIREYIQLLANQGTGD
                     ELLREFEELWAEVDRSPGGSNRGYKFNREELYEERLGRYKGKRG"
     gene            1531..1941
                     /locus_tag="VF516_42545"
     CDS             1531..1941
                     /locus_tag="VF516_42545"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEX8114506.1"
                     /translation="MAERVFIDTNVVVYACDRTAGDKRIRAREVLAPLLRERRAVIST
                     QVLQEFFAATRKLNIPAEQARYYVERWSRLDVVVIRPEVILGAIDLHRLHMLSFWDAL
                     VIKSASVAGCGQLLSEDLNHGQKIDSVTIENPFR"
     gene            complement(1993..3351)
                     /locus_tag="VF516_42550"
     CDS             complement(1993..3351)
                     /locus_tag="VF516_42550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114507.1"
                     /translation="MDIAPPESTKPAAAASSADEQSKLGRFIMRYHTFLSSFVIGVAG
                     LIATSIWQYRQSETTRNQAEAQQKVAETTAENSWKITRADILSKNLETLASSGPDSVS
                     KRYGVLLSLTRADIIDPELAVSYALELGKDNAEDMTSVLASTRHKDYGRLARAFTLSC
                     EEKYGINRTLDACDDKLAARSAAIGKLFAADAEAAFKGGEPGPMALLDDERQVQVDVQ
                     QYVGVFRTALTDMYEHREWDTISKFAAHSPGAHLISALVLAAARTGEFVTADEAKQLD
                     AFHGQQTAWLTGYFAGKTCDPECKGRLLEVMVSHYYEAQGDFDAAVRALLVGPRASSG
                     LAVSRLHARLLWCQVDDHDLDPLRDHALVPAAEQVLDPKVDAAIREAVISLLALVPAP
                     APGDEAAPAWAALTAHIDKAGPKVTKMLKDRRDIAAHERIAPPVALRNADFCGAPETP
                     AP"
     gene            3494..4273
                     /locus_tag="VF516_42555"
     CDS             3494..4273
                     /locus_tag="VF516_42555"
                     /inference="COORDINATES: protein motif:HMM:NF015945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEX8114508.1"
                     /translation="MIFGKFFGAIRAQLNKLANFFWEADPIAQMQYEYDLAVEQLKEG
                     RQGLEQYRALVERVTRQVKDGEAQVAKLTAQAKAYLKAGDRATAGNIALQLTRARAQL
                     EENRQQLGMHETAYNNNLKKIQHANKKLVEVKDKIQKYDADLKMSAAEAEVAKLTQSL
                     NLDVTTDFGQLEDVIQRKIDANRGKARVAADLSTEGLDKIQAEERMEQSMADDALKDL
                     EVELGMRAPETTPVAAQVKDLGPAEEAAAERKQPEKQTEKA"
     gene            4289..6046
                     /locus_tag="VF516_42560"
     CDS             4289..6046
                     /locus_tag="VF516_42560"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding/OmpA
                     family protein"
                     /protein_id="HEX8114509.1"
                     /translation="MANGAGKPKPAFFLAVLVVVAGLVGLSFYRWNSKKTDSGHGTGS
                     SDKIDMSDIKKQASGGAGSGSQQAENPDPNSITTVKEYKFEPASRLPEVPGTADYTPL
                     GKPRVVKFAVNVWAGWAPIIWANQGSKPKKAWKDAKGNEFQVELVLADNPVDMGNTFA
                     AGKVHIGWATVDMLPLIVNRLNRDPRTMPRVYQQIDWSNGGDGVVVREGIKSVSDLRG
                     KNVVLAQNSPSHYFLLNMLLNGGVQPSEVHMDFTKDAFQAAAAFNQDKKYAAVVSWSP
                     DIYNLTKQGMGNRMLVSTQTANRLIADVWFARADFARDNPEVIEGLVRGILDADEELK
                     TDASKQAVGKLMDEFYSLPPGTGFTMLGDAHWTNYAENRDFFLNQNNPTNFERTYNTA
                     FLLYKAIRAVDQKTPFDQIMDFSVIKKLGGEPKYAEQKNEYEFKFTPTTGIDVNVESA
                     VLTKTVTINFFPNSFDINKKVPAKTGEGEVLYDPNVEYTIEEIAKLAGQFGAARIQIS
                     GHTDASMRGVADESLVRELSLRRANAVKEALVNKFKMNPNQFVVAGYGWDKPADPKDP
                     DNHAKNRRVEIKVVPAEAQ"
BASE COUNT         1103 a         2031 c         2087 g          906 t
ORIGIN      
        1 cgagctggac gagctgctcg gcaccaagct ccgcgcgctc tatcagcgca agaagggacg
       61 agatctgttc gatctcgcgc tggcgctgga gcggcaggtg gcctcacccg atcgcatcgt
      121 cgaggtcttt gctcgttaca cgcacgaggg cggagcacgg atcacgcggg cgatgttcga
      181 gcagaacctc gcggcaaagc gagcagaccc gatcttcacc gcagacctga cgccgctgct
      241 cgccaacggt cacgcgtgga gcttcgatgc cgcgtacgat ctcgtgtggc gagagctgat
      301 cagccggctt ccaggtgagc cgtggaaggg cggttgagcg agccaggtca acggacgaat
      361 gccgtttccg atcggaggtc tcatcgtgcc tcacgcgtcg gcgaatgggt cgcgatagac
      421 ctcggcgacc gagagatcgc aagcaatcgc tgccagccgc gccgattgcc cggcaccggc
      481 gatgtggcga gaccacgtgc cgtcggcttc gcggcgcacg acctcgatct ccgggcgatc
      541 gtgcgccacg agcacgatct cctccaggct ggggatcgtc tggtagtgcg acagcttctc
      601 gccgcgatcg tagttctcgg tccgggggct cagcacctcg acgatgacgc gcgggttgat
      661 cgccgtgtgc ccggtgcggt cgtcaaggtc gcgctccaag cggccgcaga tgaccgtgac
      721 atccgggtag gttcccagcc cggtcgcttt cacgcgcacg cgcaactcgg tgctgtggac
      781 ccggcagcgt tgcccgcgta gctgcacgtt gagcagcgtg gtcacgttgc cgatgatcgc
      841 cgcgtgctcc ggagacccgc cggccatggc ccaggcttcg ccgtcgagga actcgtgctt
      901 gacctcggcg atctcctcca gcaggaggta ctcgtcgaag ctgaagcgct ggcgcgcggg
      961 cacgcccatc accaacagct tatcaccctg gaggcagtcg ggctcggcga ccacgagcag
     1021 cttcgcgacc cgagggccgg cgccgtcggg gttatcctga ctcgacgacg ccgagcaact
     1081 catcaatcgc cagccgcact cgcgcccgcc gcggcgaagt cgcctgctgc ggaggtgctc
     1141 atgctgcagc gcccgccact tgcgccggct gccgcgcgag gtcgtcggcc ggcggtcgcg
     1201 catcggggca cccgcgtgcc ctgccagctg gcacagttga cacgtaatcc gtgggcacgt
     1261 aatatgtgct ggtgaacctg accctgtccc tggacgaacg cctcgtcgaa cgagcgcgcg
     1321 aggtcgcccg ccagcaaggc acgagcttga atgcactgat tcgcgagtac atccagctgc
     1381 tggccaacca gggcacgggg gacgagctgc tccgggaatt cgaggagctc tgggccgaag
     1441 tggatcgatc gcccggagga tcgaatcgcg ggtacaagtt caatcgcgaa gagctctacg
     1501 aagaacggct cggccgctac aagggcaagc gtggctgagc gcgtcttcat cgacaccaat
     1561 gtcgtggtct acgcgtgcga tcgaaccgcg ggtgacaagc ggattcgcgc gcgtgaggtg
     1621 ctcgctccgc tgctgcgcga gcgaagagcc gtgatctcga cgcaggtcct ccaggagttc
     1681 ttcgctgcca cgcgcaagct caacatcccg gcggagcagg cacggtacta cgtcgagagg
     1741 tggagcaggc tcgacgtggt cgtgatccgc ccggaagtca tcctcggcgc gatcgatctg
     1801 caccggcttc acatgctctc gttctgggat gcgctggtga tcaagtcggc cagcgttgcg
     1861 ggctgcggcc agcttctcag cgaggacctg aaccacgggc agaagatcga cagcgtgacg
     1921 atcgagaatc cgtttcggta gacggtccga tcgcgcccgc gcgcgacttc tggcagccgg
     1981 ctcggcgatc gctcacggcg ccggggtctc cggcgcgccg cagaagtcgg cgttgcgcag
     2041 cgcgaccggc ggcgcgatcc gctcgtgcgc ggcgatgtcg cgccggtcct tgagcatctt
     2101 ggtgaccttc gggccggcct tgtcgatgtg ggcggtgagc gcggcccacg ccggcgcggc
     2161 ctcgtcgccg ggcgccggcg ccgggaccag cgcgagcagg ctgatcacgg cctcgcggat
     2221 cgcggcatcg accttggggt cgagcacctg ctcggcggcc ggcaccagcg cgtgatcgcg
     2281 cagcgggtcc aggtcgtggt cgtcgacctg gcaccacagc aggcgcgcgt ggagccgcga
     2341 caccgcgagc cccgacgacg cgcgcgggcc gaccagcagc gcgcggaccg cggcgtcgaa
     2401 gtcgccctgg gcctcgtagt agtgcgagac catgacctcg agcaggcggc ccttgcactc
     2461 cgggtcgcag gtcttgccgg cgaagtagcc ggtgagccag gcggtctgct ggccgtggaa
     2521 cgcgtcgagc tgcttggcct cgtcggcggt gacgaactcg ccggtgcgcg cggcggcgag
     2581 caccagcgcc gagatcaggt gagcgccggg cgagtgcgcg gcgaacttgc tgatcgtgtc
     2641 ccactcgcgg tgctcgtaca tgtcggtcag cgcggtccgg aacaccccga cgtactgctg
     2701 gacatcgacc tggacctggc gctcgtcgtc gagcagcgcc atcgggccgg gctcgccgcc
     2761 cttgaacgcg gcctcggcgt cggccgcgaa cagcttgccg atcgccgccg agcgcgcggc
     2821 gagcttgtcg tcgcacgcgt cgagcgtgcg gttgatgccg tacttctcct cgcacgacag
     2881 cgtgaacgcg cgggccagcc ggccgtagtc cttgtgccgg gtgctggcca gcaccgaggt
     2941 catgtcctcg gcgttgtcct tgccgagctc gagcgcgtac gacaccgcga gctcgggatc
     3001 gatgatatcg gcgcgggtca gcgacaggag cacgccgtag cgcttcgaca cgctgtcggg
     3061 gccgctcgac gccagggtct ccaggttctt ggacaggatg tcggcgcgcg tgatcttcca
     3121 gctgttctcg gcggtggtct cggcgacctt ctgctgggcc tcggcctggt tgcgcgtggt
     3181 ctcggactgg cggtactgcc agatcgacgt cgcgatcagc ccggccacgc cgatcacgaa
     3241 gctcgacagg aacgtgtggt accgcatgat gaagcggccg agcttcgact gctcgtcggc
     3301 gctggaagct gctgcggccg gcttcgtaga ctccggggga gcgatgtcca tgcgtagatc
     3361 taacccgcat ctcgtgttcg atttcccctt gtcggcggcc aggaaatgat caggttgggt
     3421 tgagtgatgt cggggacgta gctaccttta ggctcccgga gccgaaggtg cttcggggcg
     3481 gagggccacg cgcatgattt tcggcaagtt ctttggtgcg atccgggcac agctcaacaa
     3541 gctcgccaac ttcttctggg aggccgatcc gatcgcccag atgcagtacg agtacgatct
     3601 ggccgtcgag cagctcaagg agggccgcca gggcctggag cagtaccgag cgctggtcga
     3661 gcgagtcacc cgccaggtca aggacggcga ggcccaggtc gccaagctga ccgcgcaggc
     3721 caaggcctac ctcaaggccg gcgatcgcgc gaccgcgggc aacatcgcgc tccagctgac
     3781 cagggccagg gcgcagctcg aggagaaccg ccagcagctc ggaatgcacg agaccgcgta
     3841 caacaacaac ctcaagaaga tccagcacgc caacaagaag ctcgtcgagg tcaaggacaa
     3901 gatccagaag tacgacgccg acctcaagat gagcgcggcc gaggccgagg tcgccaagct
     3961 gacccagagc ctcaacctcg acgtcaccac cgacttcggc cagctcgagg acgtcatcca
     4021 gcgcaagatc gacgccaacc gcggcaaggc gcgggtcgcc gccgacctgt cgaccgaggg
     4081 cctcgacaag atccaggccg aggagcggat ggagcaatcg atggccgacg acgcgctcaa
     4141 ggacctcgag gtcgagctcg gcatgcgcgc ccccgagacc acgccggtcg cggcccaggt
     4201 caaggacctc gggcccgccg aggaggccgc cgcggagcgc aagcaacccg agaagcagac
     4261 cgagaaagcg taagtcggga gaacggacat ggccaacgga gctggaaaac ccaaaccggc
     4321 attcttcctc gcggtgctcg tcgtcgtggc gggcctcgtg gggctatcgt tctatcgctg
     4381 gaacagcaag aagaccgatt ccggacatgg aacaggctcg tccgacaaga tcgacatgtc
     4441 ggacatcaag aagcaggcca gcggcggcgc gggctccggc tcgcagcagg ccgagaaccc
     4501 ggatcccaac agcatcacca cggtcaagga gtacaagttc gagccggcgt cgcggctgcc
     4561 cgaggtgccg ggcaccgccg actacacgcc gctcggcaag ccgcgggtgg tcaagttcgc
     4621 ggtcaacgtg tgggccggct gggcgccgat catctgggcc aaccagggtt ccaagcccaa
     4681 gaaggcgtgg aaggacgcca agggcaacga gttccaggtc gagctcgtgc tcgccgacaa
     4741 cccggtcgac atgggcaaca cgttcgccgc cggcaaggtc cacatcggct gggcgacggt
     4801 cgacatgctg ccgctgatcg tcaaccggct caaccgcgat ccgcgcacga tgccgcgggt
     4861 ctaccagcag atcgactggt cgaacggcgg cgacggcgtc gtggtccgcg aagggatcaa
     4921 gagcgtcagc gacctgcgcg gcaagaacgt cgtgctcgcg cagaactcgc cgtcgcacta
     4981 cttcctgctc aacatgctgc tcaacggcgg cgtccagccc agcgaggtcc acatggactt
     5041 caccaaggac gcgttccagg ccgccgcggc gttcaaccag gacaagaagt acgcggcggt
     5101 ggtcagctgg tcgcccgaca tctacaacct gaccaagcag ggcatgggca accggatgct
     5161 ggtgtcgacc cagaccgcga accggctgat cgccgacgtg tggttcgcgc gcgccgactt
     5221 cgcgcgcgac aaccccgagg tgatcgaggg cctggtgcgc ggcatcctcg acgccgacga
     5281 ggagctcaag accgacgcca gcaagcaggc ggtcggcaag ctgatggacg agttctactc
     5341 gctgccgccg ggcaccgggt tcaccatgct cggcgacgcg cactggacca actacgccga
     5401 gaaccgcgac ttcttcctca accagaacaa cccgaccaac ttcgagcgga cctacaacac
     5461 cgcgttcctg ctctacaagg cgatccgcgc ggtcgaccag aagacgccgt tcgaccagat
     5521 catggacttc tcggtgatca agaagctcgg cggcgagccg aagtacgccg agcagaagaa
     5581 cgagtacgag ttcaagttca cgccgaccac cgggatcgac gtcaacgtcg agagcgcggt
     5641 gctgaccaag acggtcacga tcaacttctt ccccaactcg ttcgacatca acaagaaggt
     5701 cccggccaag accggcgagg gcgaggtgct gtacgacccc aacgtcgagt acacgatcga
     5761 ggagatcgcc aagctcgccg gccagttcgg cgcggcgcgg atccagatct cggggcacac
     5821 cgacgcgtcg atgcgggggg tcgccgacga gagcctggtg cgcgagctgt cgctgcgccg
     5881 ggccaacgcg gtcaaggagg cgctggtcaa caagttcaag atgaacccga accagttcgt
     5941 cgtcgccggc tacggctggg acaagcccgc cgaccccaag gaccccgaca accacgccaa
     6001 gaaccggcgg gtcgagatca aggtggtgcc ggcggaggcg cagtaggtga ccggcccggc
     6061 ccggcccgag ccgccgcgcg atcgcgctcc gggcggcccc gggcggcccg gtgcccaggg
     6121 cgccagg
//
LOCUS       DASVOR010002306         4255 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_259399,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002306 DASVOR010000000
VERSION     DASVOR010002306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259399"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VF516_42565"
     CDS             <1..394
                     /locus_tag="VF516_42565"
                     /inference="COORDINATES: protein motif:HMM:NF042614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF6798 domain-containing protein"
                     /protein_id="HEX8114510.1"
                     /translation="TTVPAADPQELALYRWVREATPIDALFVAPPSLSLFRLLARRAI
                     IADTKSPPLQPALMVRWYHRLCAMVERADVETHEEVEQRWDRLTPDQLVRTARAFHAD
                     YIVVWATTRLPGAPVYANARFAVYPVAP"
     gene            complement(1706..3064)
                     /locus_tag="VF516_42570"
     CDS             complement(1706..3064)
                     /locus_tag="VF516_42570"
                     /inference="COORDINATES: protein motif:HMM:NF005913.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEX8114511.1"
                     /translation="MEPANQAAPGLAPLGEEARRLCQALLRMDTTNPPGNERICADYL
                     AASLAEVGYRPELIEPAPGRANLIVRHRGTGAKPPLLLTAHLDVVEADPSSWRRPPFS
                     GDEHEGCLWGRGAIDMKNMAAMCTAVMRRLAATQAKLSRDVIFAAVADEEAGCELGSK
                     FLAERHRSKVEAEFAIGEAGGFSLHLGDTTFYPVQVAEKGICWIRARVTGTPGHGSMP
                     RADSAVVRLGEALAALGKAQLPVHPTKYATDFLDALRARQPALIQPLLRLLARPRLLA
                     RIARLVPNAQAARGFAPLLSNTASPTVVRAGNKTNVIPGVAEFEIDGRTLPGQTTEDL
                     LRELRAVLGPGIELEVIRSASATVTDPIESPVYDAIKHQVEAREPGAVVVPYMIPGFT
                     DAKYFSQAGARWYGFSPVKIERDSGIRFADMFHGNDERVPIAGLVWGTEVLDAVVREV
                     CA"
     gene            complement(3087..>4255)
                     /locus_tag="VF516_42575"
     CDS             complement(3087..>4255)
                     /locus_tag="VF516_42575"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEX8114512.1"
                     /translation="PPAADAKPSSAPPAQPAAPAATPAPAPRNDYAKAENWLCLPGHN
                     AACDVNLDTTVIATSGATTVEKFAAAKAPAIDCFYVYPTVSRDPGVVATMQAKAEELA
                     VVAHQFARFAQVCRPFAPLYRQFTLTALVARMSGKPLPSDGIDPKIGYNDVVDAWNYY
                     LAHHNNGRGVVLIGHSQGSSVLIPLIKNEIEGKPVQKKLVSAILMGTRLQVPAGKDVG
                     GDFASVPLCHAANQVGCAIAYASFRASAPPAATSLFGKSAGPGMEAACVNPAALAGGS
                     GAVHAYFGTRSAIVDASSQVDWSKGKTIDTPFVSVPGLLTAECKKNDVATYLAVVVHG
                     EPGSARTSEIPGDVVVGGQVQADWGLHLIDASLFMGNLLAVVRDQAAAYARGKH"
BASE COUNT          562 a         1566 c         1539 g          588 t
ORIGIN      
        1 gaccacggtg ccggcggcgg acccccagga gctcgcgctg tatcgctggg tgcgcgaggc
       61 gacgccgatc gacgcgctgt tcgtggcgcc gccctcgctg tcgctgttcc ggctgctcgc
      121 gcgccgcgcg atcatcgccg acaccaagtc gccgccgctg cagccggcgc tgatggtgcg
      181 ctggtaccac cggctgtgcg cgatggtcga gcgcgcggac gtggagaccc acgaggaggt
      241 cgagcagcgg tgggaccggc tcacgccgga ccagctcgtg cgcaccgcgc gcgcgttcca
      301 cgccgactac atcgtcgtgt gggcgaccac gcggctgccc ggcgcgccgg tgtacgccaa
      361 cgcgcggttc gcggtgtacc ccgtggcgcc atagccgggc ttgcgccgcc cgcggcctgt
      421 gggacgaagc gccagtgcgg ctgcaggatc gagccgcaca ccggcgcggc cagcggtgcg
      481 ccgggcgtcg tgccggggcc gaggcagcgc acgtggtcga agttcatgca gcagggcggc
      541 ttgccccggc aggacgactc gtccaccgcg cagggcaccg gcccgggcgg gcggccgttc
      601 cagatcagcc cgtcgctgtc gagcacccag tactgcgccg gcgtgtcccg gcacggctcc
      661 acggagaccg gatcgtcgtt gccggggccg ctcgccaggc acgccccgcc gatcgccagg
      721 tggccacccg cgtcgagctc gacgggcggc gccgccgcgc agtccgcgag cgcgacctga
      781 ccgccggcgg tcatcgaggc gcaggtcccg gtgtcgagcg cgagcggacc gcgcgccgcc
      841 agcgccgacc gggtcgacga gtactggcgg tgcatccgcc agtcgtggtc cgggttgacc
      901 gtctggcacg acgtgccgca cggcccgcac cgcgtgtagc aggcctcgaa ggagccgatc
      961 atcggcttga ccgccgcgta gtccgcgtcg ctgagggtgg gcggctcggg atcgacgctg
     1021 tagccgcggt gccagcggat cggcccgtcg tagcgcgcgt cggccgcggc ccacagcgcg
     1081 aacgccgccg agaacaggtg cccggagtgg tcgtagccgt gggtcgaggc cagatccagc
     1141 gcgtggatct cggccggccg ggtcagcgcg atgacctcgg acagctcggc gatggtcgag
     1201 gccaccgttg cgcggccctc gatccggccg aggatcggca ggtcggggac ccggccttcc
     1261 accaggtgga gcgggctgta cacgagctcg ccgaacttct caccgtcggc gatgccgagc
     1321 gcgatcatcg ccaccggccg gtcgcgcagc cggcagtgct cggcgggcga gccggcgagc
     1381 cagacgtggc cgcagctcca gtcgcgcgag ccggtgacgc tggagtaccc catccgcgcc
     1441 gccatgaacg tgtgcgcggc gcgcgcgccg ccgtgcagct ggtcgcccga cgacacgtac
     1501 accgtggtca gcgagcgccc cgacgcgagc gcgctgtaca gctcgggctg catgaagatc
     1561 atgtcgtcgt cgaagtgggc gatgatgatc agcgtgtcgg ccggctcgac ggcgggcggg
     1621 tcgttcaggt tgtcgccgca gccggccgcg cccggaccga gcagaccgac catcccggcg
     1681 gcggccgctg cgaccgcgcc gcggatcatg cacagacctc gcggaccacg gcgtcgagca
     1741 cctcggtccc ccacaccagg ccggcgatcg gcacgcgctc gtcgttgccg tggaacatgt
     1801 cggcgaaccg gatcccggag tcgcgctcga tcttgaccgg cgagaacccg taccagcgcg
     1861 ccccggcctg cgagaagtac ttggcatcgg tgaagcccgg gatcatgtac ggcaccacga
     1921 ccgcgccggg ctcgcgggcc tcgacctggt gcttgatcgc gtcgtacacc ggcgactcga
     1981 tgggatcggt caccgtcgcc gacgccgacc ggatcacctc gagctcgatg ccgggcccga
     2041 gcaccgcgcg cagctcgcgc agcaggtcct cggtggtctg gccgggcagc gtgcggccat
     2101 cgatctcgaa ctcggcgacc ccggggatca cgttggtctt gttgccggcg cgcaccaccg
     2161 tcggcgatgc ggtgttgctg agcaggggcg cgaagccgcg cgcggcctgc gcgttgggca
     2221 ccagccgtgc gatccgggcg agcagccggg gccgggccag gagccggagc agcggctgga
     2281 tcagcgcggg ctggcgcgcg cgcaaggcgt cgaggaagtc ggtggcgtac ttcgtgggat
     2341 ggaccggcag ctgggccttg ccgagcgccg ccagcgcctc gccgagccgg accaccgcgc
     2401 tgtcggcgcg cggcatcgac ccgtggcccg gcgtgccggt gacccgcgcg cggatccagc
     2461 agatcccctt ctcggcgacc tgcaccgggt agaacgtcgt gtcgccgagg tggagcgaga
     2521 acccgcccgc ctcgccgatc gcaaactcgg cctcgacctt gctccggtgg cgctcggcca
     2581 ggaacttcga gccgagctcg cagcccgcct cctcgtcggc caccgccgcg aagatcacgt
     2641 cgcgcgacag cttcgcctgg gtcgccgcga gccgccgcat caccgccgtg cacatcgccg
     2701 ccatgttctt catgtcgatc gcgccgcggc cccacaggca gccctcgtgc tcgtcgcccg
     2761 agaacggcgg gcgccgccag ctcgacggat cggcctcgac cacgtcgagg tgcgcggtga
     2821 gcaagagcgg cggcttggcc ccggtgccgc ggtggcgcac gatcaggttc gcgcggccgg
     2881 gcgccggctc gatcagctcg ggccggtagc cgacctcggc gagcgacgcc gcgaggtaat
     2941 ccgcgcagat ccgctcgttg ccgggcgggt tcgtggtgtc catgcgcagc agcgcctggc
     3001 acagcctccg ggcttcctcg ccgagcggcg ccagcccggg cgcggcctgg tttgcgggct
     3061 ccatgcgcgg actctacacg cgatggtcag tgcttcccac gggcgtacgc cgcggcctga
     3121 tcgcgcacga ctgccagcaa gttccccatg aacaggctcg cgtcgatcag atgcagaccc
     3181 cagtcggcct ggacctgacc gccgaccacc acgtcgccgg ggatctccga tgtgcgcgcc
     3241 gaaccgggct cgccgtgcac gacgaccgcg aggtacgtcg ccacgtcgtt cttcttgcac
     3301 tcggcggtga gcaggccggg cacgctgacg aacggcgtgt cgatcgtctt gcccttgctc
     3361 cagtcgacct gcgagctggc gtcgacgatc gcgctgcgcg tcccgaagta cgcgtgcacc
     3421 gcgcccgatc cacccgcgag cgccgccggg ttgacgcacg ccgcctccat ccccggcccg
     3481 gccgacttgc cgaacaggct ggtcgccgcg ggcggcgccg acgcgcgaaa cgacgcgtac
     3541 gcgatcgcgc agccgacctg gttcgccgca tggcacagcg gcaccgacgc gaagtcgccg
     3601 ccgacgtcct tgccggccgg cacctgcagc cgcgtgccca tcaagatcgc cgacaccagc
     3661 ttcttctgca ccggcttgcc ctcgatctcg ttcttgatca gcgggatcag cacgctggag
     3721 ccctggctgt gcccgatcag caccacgccg cggccgttgt tgtggtgcgc caggtagtag
     3781 ttccaggcgt cgaccacgtc gttgtagccg atcttcgggt cgatcccgtc ggacggcagc
     3841 ggcttgcccg acatccgggc gaccagcgcg gtcagcgtga actggcggta cagcggcgcg
     3901 aacggccggc acacctgcgc gaaccgcgcg aactgatgcg ccaccaccgc gagctcctcg
     3961 gccttggcct gcatcgtggc cacgacgccg ggatcgcgcg acaccgtcgg gtagacgtag
     4021 aagcagtcga tcgccggcgc cttcgcggcc gcgaacttct cgaccgtcgt ggcgccgctg
     4081 gtcgcgatca ccgtcgtgtc caggttcacg tcgcacgccg cgttgtgccc gggcaggcac
     4141 agccagttct cggccttcgc gtagtcgttg cgcggcgcgg gcgcgggcgt tgccgctggc
     4201 gctgccggtt gcgccggtgg cgcgctcgac ggcttcgcgt cggcggccgg tggct
//
LOCUS       DASVOR010002307         4267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_521440,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002307 DASVOR010000000
VERSION     DASVOR010002307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521440"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="VF516_42580"
     CDS             complement(<1..694)
                     /locus_tag="VF516_42580"
                     /inference="COORDINATES: protein motif:HMM:NF013357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HEX8114513.1"
                     /translation="MKTPLIHCYRSIARLLAVATLAGCADLPPDIDSVGEDVITCATG
                     STIKGVDVSHFDGTVDWGAAKRDGIKFAIIKATEGTSFVDNHFAANWANTRANGIVHG
                     AYHFFRPESDPVAQADFFVQVAGSPKSGELPPVIDLEVTDGRTAAQVAAGARTFLQRL
                     QQKTGRVPMIYTSVRVFNSLLGGPPGFNPYSLWVANWNVRCPNIPNPPWTRWTFWQSS
                     ATGTVAGFSDPVD"
     gene            1303..3570
                     /locus_tag="VF516_42585"
     CDS             1303..3570
                     /locus_tag="VF516_42585"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEX8114514.1"
                     /translation="MLSLALTALVAAPAPMQAAPGAANLETQFDAAISPAEMGDWMKT
                     MAAEPNHVSSAHDKLNAEMTLKQLKDWGWDAKLETFKVLYPTPIQVGLELLGRERFKA
                     TLTEAPIPGDATSSHTKDELPAYVAFQGDGDVTAPLVYVNYGMPDDYKALERLGVDVK
                     GKIVIARYGQGWRGLKPKLAHEHGAVGCIIYSDPHDDGYAIDDVYPKGAGRPAQGFQR
                     GSVADMPLYPGDPLTPGVGATDGARRLTREAAPTILKLPVLPISYGDAEHFLSALEGR
                     VVPGNWRGSLAITYHVGGTDAAKAHLVVKSDWSLKTLYDVVAVMKGSELPDQWVLRGN
                     HHDGWVFGASDPLSGHVAMMAEAKAIGQLVRSGWKPKRTLVYLSWDGEEPMLLGSTEW
                     AETHAAELKKKALVYINTDNNDRGFLRVEGSHSLEHYVARVATDVVDPQTGVSVDARR
                     RARAMTASGSAGASERGKEVGKIAADPAKEYPLEPLGSGSDYSSFLQHLGIASLNVGY
                     GGEGNGGGVYHSAYDTWEHHSRFVDPGFAYAGALAKTAGRLVLRMSEAELPVQRYGDF
                     ANTVSTYLDEVKKLADRKRDEQVAQAKMFAANAFKLSDDPTLSRGLPAPLKVVPYFNF
                     APIENAIVRLKASAKAYDAALAAKGAALPAARKSQLVELAGRTEQALLMEPGLPGGRG
                     WYKNMIYAPGRLTGYGAKTLPGVREAIEDERWTDVDTYAAMIGKALTAYADRLDEGVA
                     LINGPSASARAPGAN"
     gene            4087..>4267
                     /locus_tag="VF516_42590"
     CDS             4087..>4267
                     /locus_tag="VF516_42590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114515.1"
                     /translation="MDEASAPGVPLGRYRLIALLGQGGMADVYLACTQGAGGFRKLLV
                     VKLARFTGEPLFATMF"
BASE COUNT          754 a         1407 c         1423 g          683 t
ORIGIN      
        1 catcgaccgg atcgctgaat ccagcgacgg tgccggtggc agaggattgc cagaaggtcc
       61 agcgcgtcca tggtgggttt ggaatgttcg ggcatcgcac gttccagttc gcgacccaga
      121 gggaatacgg attgaagccg ggcggccccc cgagcagcga gttgaagacg cgtacggacg
      181 tgtagatcat cggcacccgc ccggtcttct gctgcaggcg ctggaggaac gtccgtgctc
      241 ctgccgccac ctgggccgcg gtccgcccat cggtcacttc gagatcgatg accggcggca
      301 attcgcccga cttcgggctg cctgcgacct ggacgaagaa atcggcctga gcaactggat
      361 cggactccgg cctgaagaag tgatacgcgc cgtgaacaat tccattggct cgcgtattcg
      421 cccagttggc cgcaaagtga ttatcgacga acgacgtacc ctcggtcgcc ttgatgatcg
      481 cgaacttgat tccatcccgc ttggcggccc cccaatcgac cgtcccgtca aagtgggaga
      541 cgtcgacacc tttgatcgtg gaaccagttg cgcaggtgat aacgtcttct ccgaccgaat
      601 cgatatctgg aggaaggtcc gcgcacccgg ccagcgtggc aacggcaagc agtcgtgcga
      661 tcgatctgta acaatgaatg agcggtgttt tcatgtgcgg ccgcttttac gagcggccat
      721 tcaacgagtc aatgtacgac atgtcctaca atacatgtcg actattacgt aagagaaact
      781 ctcgctatgc aagcgttcag gtttgtgccg ctcgcgagcg tattggggat cacgaacaac
      841 gattttcctg agcactaaaa tctgctcgca acgaacgcag ccttctgaca agcgggcatc
      901 cgcggggtag ccggcacgcg tggtaccccg ggcgaggcgc ggcgctgggg ccacgcgttc
      961 gaggcggcct tcctcgccca cgaggcgcgg gccgagcgcg gcgagccgag cctgctccgc
     1021 gatgacgccg tgacgaacga ggcggagtat gttcgccgtc gcgacggagg cgttcttcga
     1081 gcgccccggc gagctggccg ccgagcttcc ggagtctacg ccgcgctgcg tgacttctac
     1141 gggctcgacc tggcgcgtcc gagataaaac ccaccccccg aggacggttg tcctggcgcg
     1201 tcgaacgacc gagtgccggg cagcggtctt gttcgcgcgg acgaaagggc cacccgggcg
     1261 ctcatcctgt gcgagtctct cgccatgccc cgatccctat ccttgctctc tctcgcactc
     1321 accgcgctgg ttgccgcgcc cgccccgatg caggccgcgc ccggcgcggc caatctcgag
     1381 acgcagttcg acgcggcgat cagtcccgcc gagatgggcg actggatgaa gaccatggcg
     1441 gccgagccca accacgtcag ctcggcccac gacaagctca acgccgagat gacgctgaag
     1501 cagctcaagg actggggctg ggacgcgaag ctcgagacct tcaaggtgct ctacccgacg
     1561 ccgatccagg tcgggctcga gctcctcggc agggagcgct tcaaggcgac gctgaccgaa
     1621 gcgcccatcc ccggcgacgc cacctcctcc cacacgaagg acgagctgcc ggcctatgtc
     1681 gccttccagg gcgacggcga tgtcacggcg ccgctggtct acgtgaacta cggcatgccc
     1741 gacgactaca aggccctcga gcgcctgggc gtcgacgtca agggcaagat cgtcatcgca
     1801 cgctacggcc agggctggcg ggggctcaag ccgaagctgg cgcacgagca tggcgcggtc
     1861 ggctgcatca tctactcgga tccgcacgac gacggctacg cgatcgacga cgtctatccg
     1921 aagggtgcgg ggcgcccggc gcagggattt cagcggggct cggtcgccga catgccgctc
     1981 tatccaggcg atccgttgac cccgggcgtg ggcgccaccg acggcgccag acgcctgacc
     2041 cgcgaagccg cgccgacgat cctgaagctc ccggtgttgc cgatctcgta cggggacgcc
     2101 gagcatttcc tctcggcgct cgaaggccgc gtggtgcccg gcaactggcg tggcagcctg
     2161 gcgatcacct accacgtcgg cggcaccgac gccgccaagg cgcacctcgt ggtcaagtcc
     2221 gactggtcgc tgaagacgct ctacgacgtc gtcgcggtca tgaagggctc cgagctcccc
     2281 gatcagtggg tgctacgcgg caaccaccat gacggctggg tgttcggcgc ctccgatccg
     2341 ctcagcggcc acgtcgcgat gatggccgag gccaaggcga tcggccagct ggtcaggtcc
     2401 ggctggaagc cgaagcgcac gctggtctac ctcagctggg acggcgagga gccgatgctt
     2461 ctcggctcca ccgaatgggc cgagacccac gcggcggagc tcaagaagaa ggcgctcgtc
     2521 tacatcaaca ccgacaataa cgaccgcggc ttcctccgcg tcgagggcag ccattccctg
     2581 gagcactatg tggcgagggt tgcgaccgat gtcgtcgatc cgcagaccgg cgtctcggtg
     2641 gacgcccgcc gccgcgcccg tgcgatgacc gccagcggat ccgccggggc gagcgaacgc
     2701 ggcaaggagg tcggcaagat cgccgcagat ccggccaagg agtacccgct cgagccgctc
     2761 gggtcgggct cggactattc cagcttcctg cagcacctcg gtatcgcatc gctcaacgtc
     2821 ggctatggcg gcgagggcaa cggcggaggt gtctaccact ccgcctacga cacctgggag
     2881 catcactcga gattcgtcga tccgggcttc gcctatgccg gcgcgctcgc gaagaccgcg
     2941 gggcggctgg tcctgcgcat gtcggaggcg gagctgccgg ttcagcgcta cggtgacttc
     3001 gcgaacaccg tctcgaccta tctcgacgag gtgaagaagc tggctgatcg caagcgcgac
     3061 gagcaggtcg cgcaggccaa gatgttcgcc gcgaatgcgt tcaagctgtc agacgatcca
     3121 acgctcagcc gcgggctgcc agccccgctg aaggtcgtgc cctacttcaa cttcgcgccg
     3181 atcgagaacg cgatcgtacg cctgaaggcc agcgcaaagg cgtatgatgc cgcgctcgcc
     3241 gcgaagggcg cggcgttgcc cgcggccagg aagagccagc tggtcgagct cgccgggcgg
     3301 accgagcagg cgttgctgat ggagcccggc ttgccgggcg ggcgcggctg gtacaagaac
     3361 atgatctacg cgcccggccg cctcaccggc tatggcgcga agacgctgcc cggcgtccgc
     3421 gaggcgatcg aggacgagcg ctggacggat gtcgacacgt acgccgccat gatcggcaag
     3481 gcgctcaccg cgtatgccga ccgcctcgat gaaggcgtcg cgttgatcaa cggcccgtcc
     3541 gcttcggcca gggctccggg agcgaactga cggggccgga ccggtacccg caggacatga
     3601 accttgctcg actcgcgtgg tgtcatcacc gggccccagc ctctacaatg catttcagga
     3661 gtcgagtccg gaacataagg gtgctggtcc gaggggacag cggaatcggc tgaccggcat
     3721 cgcggcgatc gtgtctcgcc gtcgcggcga tcggcggggc cgcggtggtg tcggccgaca
     3781 gggcggtcgg cgcaggccct gacgcatcgc gctatcgcat cggtaggcac gattacgccg
     3841 caacccataa tcgattacca ccccgcaacg cgggtggccg tcgcccatcc aaggtgccgc
     3901 gcaacgacgc atgacgggac ggaccgcgcc tggtcgagtc acaccgccat cggtgtggct
     3961 gactcttcga gtaaagcctt gcctgcgggc gacactctgg agcacgctct gcaagctagt
     4021 aaactccgcg cgtggagggc gctgcggagc tgctgctgag aggtacagac gtcgatgagg
     4081 acatccttgg acgaagcgtc ggcaccgggg gtgccgctcg ggcgataccg gttgatcgcg
     4141 ttgctcggcc agggcggcat ggccgatgtc tacctggctt gcacacaggg cgccggcggg
     4201 ttccggaagc tgctcgtggt caagctggcg cggttcaccg gcgagccgct gttcgccacg
     4261 atgtttc
//
LOCUS       DASVOR010002308         1592 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_345978,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002308 DASVOR010000000
VERSION     DASVOR010002308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1592
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345978"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..675
                     /locus_tag="VF516_42595"
     CDS             <1..675
                     /locus_tag="VF516_42595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114516.1"
                     /translation="VAAITAVAAITGIAAAAPAARAEPSDRALVLTGLALAPPTYLVG
                     VSLHEGSHALAAVLVGATVDTLHLFPPGRDPSTGTFRFGWTYVRGLGSDGDKLAFYAA
                     PKLTDAALLGGFAALAFTGAWPGNRYGQLALTVFATGLWVDFSKDVVLFAPTNDVVKV
                     LDLAGLTGWRELPARLAYAGMVVGLGLIVAHGYRRTFDAPAATFPAPAAPAAMPRIVP
                     LAAIAF"
     gene            782..1276
                     /locus_tag="VF516_42600"
     CDS             782..1276
                     /locus_tag="VF516_42600"
                     /inference="COORDINATES: protein motif:HMM:NF021621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HEX8114517.1"
                     /translation="MKRACIAIVDAARARIYTYDQTTDGGRSAPAGALHEAIDLVNPG
                     RRGHDPYSTTKPGVKRNAPGSGTTDDHRDAHADELDRRFARQVVSEIDRIAREHAYDR
                     VLLVASPAMLGELRGVDRELRPELQVEYVARDLARLTSPQIHDHLAQLRLVAPRRPAS
                     VVRG"
     gene            1402..>1592
                     /locus_tag="VF516_42605"
     CDS             1402..>1592
                     /locus_tag="VF516_42605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114518.1"
                     /translation="MSTTRLALCLLLAGLVAGCATNSNGDDGSNGGDGGMTGDDAPPF
                     TNGVSTLTGAAMAGYVDGAR"
BASE COUNT          201 a          597 c          577 g          217 t
ORIGIN      
        1 gtcgcggcga tcaccgcggt cgcggcgatc accgggatcg cggcggcggc gcccgcagcg
       61 cgcgccgagc cgagcgatcg cgcgctggtg ctgaccgggc tggcgctggc gccgccgacc
      121 tacctggtcg gggtctcgct ccacgagggc agccacgcgc tcgccgcggt gctggtcggc
      181 gccacggtcg acaccctgca cctgttcccg cccggccggg acccgagcac gggcacgttc
      241 cggttcggct ggacctacgt ccgcggcctg ggctcggacg gcgacaagct ggcgttctac
      301 gccgcgccca agctcaccga cgccgcgctg ctcggcgggt tcgccgcgct ggcgttcacc
      361 ggcgcgtggc cgggcaaccg ctacgggcag ctcgcgctga ccgtgttcgc gaccgggctg
      421 tgggtcgact tctcgaagga cgtggtgctg ttcgcgccga ccaacgacgt ggtgaaggtg
      481 ctcgacctcg ccgggctgac cggctggcgc gagctgccgg cccggctcgc ttacgccggg
      541 atggtggtcg ggctcggcct gatcgtggcg cacggctacc ggcgcacgtt cgacgcgccc
      601 gccgcgacgt tcccagcccc ggcggcgccg gcggcgatgc cgcggatcgt gccgctggcc
      661 gcgatcgcgt tctagcgact acgagttctc gcggcgtgcg ccgccgcgcg ctgcgcggtc
      721 cgaccgcgca ccgccggaat gctgcttgca ccgctcctcg ggcacccggg aggtgcgtcc
      781 catgaaacga gcttgcattg cgatcgtcga tgccgcgcgg gcgcggatct acacctatga
      841 tcagaccacg gatggcgggc gatcggcacc ggcgggggcg ctgcacgagg cgatcgatct
      901 ggtgaacccg ggccgccgcg gccacgaccc gtactcgacg accaagcccg gggtcaagcg
      961 caacgcgccg ggctcgggga cgaccgacga tcaccgcgac gcgcacgccg acgagctcga
     1021 tcgccggttc gcccgccagg tggtctcgga gatcgaccgg atcgcgcgcg agcacgccta
     1081 cgaccgggtc ctgctggtcg cgagcccggc gatgctcggc gagctgcgcg gcgtcgatcg
     1141 cgagctgcgt cccgagctcc aggtcgagta cgtcgcgcgc gacctggcgc ggctgacctc
     1201 gccccagatc cacgatcacc tggcgcagct gcggctggtc gcgccgcgcc ggccggcgtc
     1261 ggtcgtccgc ggctgagcgg tgcacgcgca agcgagcgcc atcgcacgcc gcgcgctcag
     1321 gtttcgacca cctgcgcgta atcgcgctcg gcaagcggct tgctcttctg gctgcaagcc
     1381 aactacctcc gaaggtccct tatgagcacc acccgactcg cgctgtgtct cctcctcgcc
     1441 ggcctggtcg ccggctgcgc cacgaactcg aacggcgacg acggcagcaa cgggggcgac
     1501 ggcggcatga ccggcgatga cgcacccccg ttcaccaacg gcgtcagcac gctgaccggc
     1561 gcggcgatgg cgggctacgt cgacggcgcg cg
//
LOCUS       DASVOR010002309         4426 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113859,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002309 DASVOR010000000
VERSION     DASVOR010002309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113859"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1865
                     /locus_tag="VF516_42610"
     CDS             <1..1865
                     /locus_tag="VF516_42610"
                     /inference="COORDINATES: protein motif:HMM:NF024928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEX8114519.1"
                     /translation="YLKDSRGSWVQAVGQIAPVLHGGMGKAARPVAAGGARGTLGIHH
                     GTQRADKALPEIAALIDKEQFDLITQPGSGIVVIQGGAGSGKTTVALHRIAYLNFQDG
                     RRFKPSHTLFVVPSQALVRYVSGVLPSLGVSGVPVVTYTGWARTTRMRCLPDSPTKYN
                     PEPPEQVSRVKKHPALLAMLERWVARQAAQIGAEIAALPPQAAATPGTGTGPGLGAQT
                     AALDEWNRLAARALVPRLSGFASWVKRAELPPHERLALEGIARRWKKRADDCVLDWAE
                     LLTDPVALAEGFAGSDVKKTDIERVVGWLRRQLARPQKAPVDEEGNPVLDSEGQPLGV
                     DEDDPAGRFDDEDDPILLRLVQLKRGGLYPPTGDELSWQHVAIDEAQDRSALEIKVLL
                     EAVHAPDGDPGKRSVTIAGDTAQRLVFDNNFSGWADLLAQTGQPAIVRPLRLSYRSTA
                     EVMVLAREILGPELASDEPLAARPGEPVELHEFGDLGEAIAFLGDALRNLMAREPTAS
                     CAVIARHPEQADAYFDGLRRAEVPALRRVRRDEFNFQPGVDVTDIAQVKGLEFDYVVM
                     VDVNAASYPDQRWARHMLHIGVTRAAHQLWLVSTGEPSELIPAALRDGGRMGVA"
     gene            complement(1997..2575)
                     /locus_tag="VF516_42615"
     CDS             complement(1997..2575)
                     /locus_tag="VF516_42615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114520.1"
                     /translation="MRVALCAVLAALLTVRGEVDACVLESPEITPHELDPAYANDTVA
                     PSIPQATFSISRHEVGGGCAQTDCDGKYARVYVEVTGGDDQTPRERLGYILTIIGGDT
                     PAHMYTAVSNGMPVFQPQGSFTYGFDYDDHEYAFDLEIRTVDLNGNVSEPTLLHIAEH
                     DAGGCSITSSVRSEWLAFPVMCFVLRSRRRRG"
     gene            complement(2927..3721)
                     /locus_tag="VF516_42620"
     CDS             complement(2927..3721)
                     /locus_tag="VF516_42620"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8114521.1"
                     /translation="MRTRRIKSADGTTIAFEAIGQGAPVVLVGGALSDRTAPASGKPL
                     AAMLAHRFTVFSYDRRGRGDSSDTPGWAIAREIEDLAAMVAAAGGSASVFGNASGGLL
                     ALDAAAQGLAITRLAVYEPPVILDAGRAASFVRLANQLDEVMATDRRREAVELYFTRV
                     MQMPAQAVTQLRRSPMWADFEDLAHTLSYDLRITARGAARLAEMPAVRPPTLALDGSA
                     SPPWMREAIAAVARAIPQGRHRTLEGQAHAVDPKELALALEEFLSA"
     gene            complement(3972..4289)
                     /locus_tag="VF516_42625"
     CDS             complement(3972..4289)
                     /locus_tag="VF516_42625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114522.1"
                     /translation="MVSLLGRLLHVEVPGFQEARLADATLNELVPVEYRADAVVVFTE
                     VKPEGKAEGEAAALLKILARRGFTLTTDQRRQVLECTDLGLLEQWLDRALSASSADEL
                     VTK"
BASE COUNT          668 a         1621 c         1501 g          636 t
ORIGIN      
        1 cgtacctcaa ggactcgcgc ggctcgtggg tgcaggcggt ggggcagatc gcgcctgtcc
       61 tgcacggcgg gatgggcaag gcggcgcggc cggtggcggc gggtggggcg cgcggcacgc
      121 tcggcatcca ccacggcacc cagcgcgccg acaaggcgct gcccgagatc gccgcgctga
      181 tcgacaagga gcagttcgat ctgatcaccc agcccggcag cggcatcgtc gtgatccagg
      241 gcggcgccgg ctcgggcaag accaccgtcg cgctgcaccg gatcgcctat ctcaacttcc
      301 aggacggccg gcggttcaag ccgagccaca cgctgttcgt ggtgccgtcg caggcgctgg
      361 tccgctacgt cagcggcgtc ctgccatcgc tcggcgtgtc cggcgtgccg gtcgtgacct
      421 acaccggctg ggcgcgcacg acccggatgc gctgcctgcc ggactcgccg accaagtaca
      481 accccgagcc gcccgagcag gtgtcgcggg tcaagaagca cccggcgctg ctcgccatgc
      541 tcgagcgctg ggtcgcgcgc caggccgcgc agatcggcgc cgagatcgcg gcattaccgc
      601 ctcaggccgc ggcgactccc gggaccggga ccggccccgg cctgggggcg cagaccgcgg
      661 cgctcgacga gtggaaccgg ctcgccgcgc gggcgctggt gccccggctg tccggcttcg
      721 ccagctgggt caagcgcgcc gagctgccgc ctcacgagcg gctcgcgctc gagggcatcg
      781 cgcggcgctg gaagaagcgc gccgacgact gcgtgctcga ctgggccgag ctgctcaccg
      841 acccggtcgc gctcgccgag ggcttcgcgg gcagcgacgt caagaagacc gacatcgagc
      901 gcgtcgtcgg ctggctgcgc cggcagctcg ccaggccgca gaaggccccg gtcgacgagg
      961 agggcaaccc ggtgctcgac tccgagggcc agccgctcgg cgtcgacgag gacgaccccg
     1021 ccggccggtt cgacgacgag gacgacccga tcctgctccg gctggtccag ctcaagcgcg
     1081 ggggccttta cccgccgacc ggcgacgagc tgagctggca gcacgtcgcg atcgacgagg
     1141 cccaggaccg ctcggcgctg gagatcaagg tcctgctcga ggccgtgcac gcccccgacg
     1201 gcgatcccgg caagcgctcg gtcacgatcg ccggcgacac cgcccagcgc ctggtgttcg
     1261 acaacaactt ctcgggctgg gccgacctct tggcccagac cggccagccc gcgatcgtcc
     1321 gcccgctgcg cctgtcctac cgctcgaccg ccgaggtcat ggtgctcgcc cgcgagatcc
     1381 tcggccccga gctcgcctcc gacgagccgc tcgccgcgcg gccgggcgag ccggtcgagc
     1441 tccacgagtt cggcgatctc ggcgaggcga tcgcgttcct cggcgacgcc ctgcgcaacc
     1501 tgatggcgcg cgagccgacc gcgtcgtgcg ccgtgatcgc gcgccacccc gagcaggccg
     1561 acgcctactt cgatggcctg cgccgcgccg aggtccccgc gctgcgccgg gtccgccgcg
     1621 acgagttcaa cttccagccc ggggtcgacg tcaccgacat cgcccaggtc aagggcctgg
     1681 agttcgacta cgtcgtcatg gtcgacgtca acgccgcgag ctaccccgat cagcgctggg
     1741 cccgccacat gctccacatc ggcgtcaccc gcgccgccca ccagctctgg ctggtctcca
     1801 ccggcgagcc cagcgagctg atcccggcgg cgctgcgcga cggcggccgg atgggcgtgg
     1861 cctgacgggg aagctcgccc ggtcatgcca cacatcgccg gctgtctggg ttgcgagatc
     1921 gttcgcgcca gccgtgacgc ggcggcgggt tcgactccca ccgcctccac gatttcattg
     1981 tcattctgcg aggacgtcaa ccacgcctgc gccgggaacg cagcacgaaa cacatgactg
     2041 gaaaggcgag ccactcactc cgcaccgacg aagttatcga gcaaccaccc gcgtcgtgct
     2101 ctgcgatatg cagcagcgtc ggttcactga cgttcccgtt cagatcaacg gtgcggatct
     2161 cgaggtcgaa cgcgtactcg tggtcgtcgt agtcgaagcc ataggtgaaa gagccctgcg
     2221 gctggaacac cggcatgccg ttcgacacgg cggtgtacat gtgcgcgggt gtatcaccgc
     2281 cgatgatcgt gaggatgtag cccagacgct ctcgtggcgt ctggtcgtcg ccgccagtca
     2341 cctcgacata cactcttgcg tacttgccat cgcaatcggt ctgtgcgcac ccaccgccca
     2401 cttcgtggcg agagatcgag aacgtcgcct gcgggatgct gggggcgacc gtatcgttgg
     2461 cataggccgg atcgagctcg tgcggcgtga tctcaggcga ctcgagtacg cacgcatcga
     2521 cttcgccgcg aaccgtgagc agagcggcta gcaccgcaca gagcgcaact cgcatgcggc
     2581 gcaaggctgc aaggtggcgg ccagcgcaag tccgcaatat cacaccccca cccgtcgcag
     2641 tctgcgacat agatgtccgg atttcgctgg ccatccaacc cgcgggggcc gacccgctcc
     2701 gaagcggcga tgtggctcac ccggcactgg cccgagctcg aggtattcgt cggtcacgag
     2761 gtccgcgcgg cgtcggctcg tgcggtgacc gcttccagcg cgaccgccgc gcctgcggcg
     2821 gattgagcgc acgctgcgcc agttccgccc gcccgctcac gtgctcgtgc gagagcatcg
     2881 cgctcgaaat gatccggatg ctgctttcct ctatcaggca cggcgctcac gcgctcaaaa
     2941 actcctcgag cgccagcgcg agctccttgg gatcgaccgc gtgggcctgg ccctccagcg
     3001 tgcggtggcg gccctgggga atcgcgcgcg ccaccgccgc gatcgcctcg cgcatccagg
     3061 gcgggctcgc gctgccgtcg agcgccagcg tcggtgggcg caccgccggc atctcggcga
     3121 gccgcgccgc cccgcgcgcc gtgatccgga ggtcgtagct cagcgtgtgg gcgaggtcct
     3181 cgaagtcggc ccacatcggc gacctgcgca gctgcgtgac cgcctgcgcg ggcatctgca
     3241 tcacccgggt gaagtacagc tcgacggcct cgcgccggcg atcggtcgcc atgacctcgt
     3301 cgagctggtt cgcgaggcga acgaacgacg ccgcccggcc tgcgtcgagg atgaccggcg
     3361 gctcgtagac cgcgaggcgc gtgatcgcca gcccctgcgc ggcggcgtcg agggccaaga
     3421 ggccgcccga cgcgttgccg aacaccgacg ccgacccacc ggccgccgcg accatcgcgg
     3481 cgaggtcctc gatctcgcgc gcgatcgccc accccggcgt gtcgctgctg tccccgcggc
     3541 cgcgccggtc atagctgaac acggtgaagc gatgcgcgag catcgccgcg agcggcttgc
     3601 ccgacgccgg ggccgtgcgg tccgacagcg cgccgcccac caggacgacc ggagcgccct
     3661 ggccgatcgc ctcgaacgcg atcgtcgtgc cgtccgccga cttgattcgc ctggtcctca
     3721 tggccctgga acagcatacg gtcctcgtcg aaaacgtccg ccggtgccgg cggaacgcct
     3781 gcgtgtgtgc gccgccgacc gtcggaggat cgatgtggga gcacggcgct ccgtgccgcg
     3841 tcagatctcg ccatgccaca tgtcgccggc tgcctcggct gcgagatcgt cgctggcctg
     3901 cgcgccgtgc cggacctcca ccacgtcctt cgatgactgc gcggagtccg ggaagcgtga
     3961 aaccacccga actactttgt gacgagctcg tcagccgagc tcgccgatag cgcccgatcg
     4021 agccattgct cgagcagccc gagatcggtg cactcgagta cctggcggcg ctggtcggtc
     4081 gtaagcgtga aaccacggcg cgccaggatc ttcagcagcg ccgccgcttc tccttcggcc
     4141 ttcccttcgg gcttgacctc ggtgaacacc acgaccgcgt cggcgcgata ctcgacgggc
     4201 accaactcgt tgagcgtggc atcggcaagt cgcgcttcct gaaatcccgg cacctcgaca
     4261 tgaagcaacc gacctagtag cgacaccatg cgatccgcaa ccaccctgtt cgcagggtct
     4321 ggccacgagg gctcgtgaac gcctcgacga cgacgaacta tggcgtcagg aagttcagcg
     4381 cctcgggcag cgggagggcg atcggggtct cgaacagcgg gccggg
//
LOCUS       DASVOR010002310         3915 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_113877,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002310 DASVOR010000000
VERSION     DASVOR010002310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3915
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113877"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..893
                     /gene="ureA"
                     /locus_tag="VF516_42630"
     CDS             228..893
                     /gene="ureA"
                     /locus_tag="VF516_42630"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF009671.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HEX8114523.1"
                     /translation="MRLVPREIDKLLLHQAGALAQKRLARGVRLNYPEAVALIATQLL
                     ELIRDGRPVAELMDIGRTLLGRRQVMDGVPELVTEVQVEGTFPDGTKLVTVHHPIVAE
                     HGDLALALYGSFLPVPDRERFAEAPRAVPVGEVTPVDGQIELNAGRPTTELVVTNTGD
                     RPIQVGSHYPFAETNRALVFDRAAAAGQRLDIPAGAAVRFEPGEQRTVRLVPGTGTAG
                     GEP"
     gene            890..2599
                     /gene="ureC"
                     /locus_tag="VF516_42635"
     CDS             890..2599
                     /gene="ureC"
                     /locus_tag="VF516_42635"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HEX8114524.1"
                     /translation="MSHTLDRRGYAELYGPTTGDRVRLGDTDLVAEVERDHTVYGDEC
                     KFGGGKVLRDRMGQYAGAEDAQALDLVITNALIVDWTGIYKADIGIKHGRIAGIGKAG
                     NPAVMAGVTPGMIVGVTTDALAGEGLIVTAGGVDSHIHFICPQQADEALASGVTTFVG
                     GGTGPTTGSNATTCSPGARHIELMLQATDALPLNIGLTGKGNTSRPEGLVDQIRAGAI
                     GLKLHEDWGTTPPAIDCCLGIAEGEDVQVTIHTDTLNESGYVDDSIAAFRGRTIHTYH
                     TEGAGGGHAPDILRVCGEPNVLPSSTNPTRPYTVNTLDEHLDMLMVCHHLDREIPEDV
                     AFADSRIRGETIAAEDILHDLGALSMISSDSQAMGRVGEVITRTWQTAHKMKQQRGPL
                     PGDRADNLRIRRYVAKYTINPAIAHGFAHEVGSVEVGKLADLVLWKPAFFGVRPELVL
                     KGGFIAWAQMGDANASIPTPEPLRMRPMFGALGRAVGATSLAFISRRAQREGQVHALG
                     LTKQLVVVRGCRGLTKRDMKLNDALPRLAIDPETYQVRADGELLTCEPATVLPLAQRY
                     HLF"
     gene            complement(2616..3518)
                     /locus_tag="VF516_42640"
     CDS             complement(2616..3518)
                     /locus_tag="VF516_42640"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8114525.1"
                     /translation="MKDVKDKVAVVTGAASGIGRGMAEVFADAGMKVVLSDIEQPALE
                     RTCSALRASGADVHAVATDVSRPEQVEQLAEQTLRTYGAVHVLCNNAGVGGSGGGQSW
                     TSSLKDWQWILGVNLMGVVHGIRTFLPIMIKQDTEAHIVNTASLAGLVPSVMTLYCTT
                     KFAVVGLSESVNLELIRGGLKPKISVLCPGWVDTNILDSQRNRPAELANDAPAPVTPV
                     GQAIREWVSEQLKQGLTPRAVGEQVLAAIRDERFYILTHPSETPVIEQRMRDIVRGNN
                     PALLPILGIESLMKRLGALNPAAR"
     gene            3687..>3915
                     /locus_tag="VF516_42645"
     CDS             3687..>3915
                     /locus_tag="VF516_42645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114526.1"
                     /translation="MPWIVLQLADSAVPIGGFAHSGGLEALVAARELGALEAFCRALI
                     DQQAHGARPLVGAAWDAPDRLAELDARSRAIL"
BASE COUNT          598 a         1431 c         1320 g          566 t
ORIGIN      
        1 cggcaccgcg cgccccggtc gtcgtcgccg gcagcccgct cgccggcggc gccgtgttcc
       61 ggatcgccgg cgagcgcgtc gatctcgtca ccgacgcggc ccgcgcgctg atcggcgagg
      121 cgtgcgccga gctcggcgcc gtcccctggc agcgccgctg gtgatcggcg ccgagaggaa
      181 acccagcgga aacccgcgca gcgctccgcg gtgatagcgt tcgcgcgatg cgtcttgtgc
      241 cccgcgagat cgacaagctg ctgctccatc aggccggcgc gctcgcgcag aagcggctgg
      301 cgcgcggcgt gcggctcaac taccccgagg cggtcgcgct gatcgcgacc cagctgctcg
      361 agctgatccg cgacggccgc ccggtggccg agctgatgga catcggccgc acgctgctcg
      421 gccgccgcca ggtgatggac ggcgtgcccg agctggtgac cgaggtccag gtcgagggca
      481 cgttccccga cggcaccaag ctggtgacgg tgcaccaccc gatcgtcgcc gagcacggcg
      541 acctcgcgct cgcgctgtac ggcagcttcc tgccggtgcc cgaccgggag cggttcgccg
      601 aggcgccgcg cgccgtgccg gtcggcgagg tgaccccggt cgacggccag atcgagctca
      661 acgccggccg gccgaccacc gagctggtcg tgaccaacac cggcgatcgg ccgatccagg
      721 tcggcagcca ctacccgttc gccgagacca accgcgcgct ggtgttcgat cgcgcggccg
      781 ccgccggcca gcggctcgat atcccggccg gcgccgccgt gcggttcgag cccggcgagc
      841 agcgcacggt gcggctggtg ccgggcacgg gaaccgcagg aggcgagccg tgagccacac
      901 cctcgatcgc cgcggctatg ccgagctcta cggcccgacc accggcgacc gggtccggct
      961 cggcgatacc gatctcgtcg ccgaggtcga gcgcgatcac acggtgtacg gcgacgagtg
     1021 caagttcggc ggcggcaagg tgctgcgcga tcgcatgggc cagtacgccg gcgccgagga
     1081 cgcccaggcg ctcgatctgg tgatcaccaa tgcgctgatc gtcgactgga ccgggatcta
     1141 caaggccgac atcggcatca agcacggccg gatcgccggg atcggcaagg ccggcaaccc
     1201 ggcggtgatg gccggcgtga cgccggggat gatcgtcggc gtcacgaccg acgcgctggc
     1261 cggcgagggc ctgatcgtca ccgcgggcgg ggtcgacagc cacatccact tcatctgtcc
     1321 gcagcaggcc gacgaggcgc tcgccagcgg cgtgaccacg ttcgtcggcg gcggcaccgg
     1381 gccgaccacc ggcagcaacg cgacgacctg ctcgccgggc gcgcgccaca tcgagctgat
     1441 gctgcaggcg accgacgcgc tgccgctcaa catcgggctg accggcaagg gcaacacgtc
     1501 caggcccgag ggcctggtcg accagatccg cgccggcgcg atcggcctca agctgcacga
     1561 ggactggggc acgacgccgc cggcgatcga ctgctgcctc ggcatcgccg agggcgagga
     1621 cgtccaggtc accattcaca ccgacacgct caacgagtcg ggctacgtcg acgactcgat
     1681 cgccgcgttc cgcggccgga cgatccacac ctatcacacc gagggcgccg gcggcggcca
     1741 cgcccccgac atcctgcggg tgtgcggcga gcccaacgtg ctgccgagct cgaccaaccc
     1801 gacccggccg tacaccgtca acacgctcga cgagcacctc gacatgctga tggtatgtca
     1861 ccacctcgat cgcgagatcc ccgaggacgt cgcgttcgcc gacagccgga tccgcggcga
     1921 gaccatcgcc gccgaggaca tcctgcacga cctcggcgcg ctgtcgatga tctccagcga
     1981 cagccaggcg atgggtcggg tcggcgaggt gatcacgcgg acctggcaga ccgcgcacaa
     2041 gatgaaacag cagcgcgggc cgctgcccgg cgaccgcgcg gacaacctgc ggatccgccg
     2101 ctacgtcgcc aagtacacga tcaacccggc gatcgcccac ggcttcgcgc acgaggtcgg
     2161 ctcggtcgag gtcggcaagc tcgccgacct ggtgctgtgg aagccggcgt tcttcggcgt
     2221 gcggcccgag ctcgtgctca agggcggctt catcgcctgg gcccagatgg gcgacgccaa
     2281 cgcctcgatc ccgacccccg agccgctgcg gatgcggccg atgttcggcg cgctcggccg
     2341 cgcggtcggc gccacctcgc tcgcgttcat ctcgcggcgc gcccagcgcg agggccaggt
     2401 ccacgcgctc ggcctgacca agcagctcgt cgtggtccgc ggctgccgag gcctgaccaa
     2461 gcgcgacatg aagctcaacg acgcgctgcc ccggctcgcg atcgatcccg agacctacca
     2521 ggtccgcgcc gacggcgagc tcctgacctg cgagccggcg accgtgctgc cgctcgccca
     2581 gcgctatcac ctgttctgag cctgccgctc gtgcgctatc gggctgctgg attcaaggcg
     2641 ccgagccgct tcatcaatga ttcgatgccg aggatcggga gcagcgctgg attgttgccg
     2701 cgaacgatat ctcgcatgcg ctgctcgatc accggagtct ccgacgggtg agtgaggatg
     2761 tagaaccgct catcgcggat cgccgccagc acctgttcgc ccaccgcgcg cggggtcaat
     2821 ccctgcttga gctgttcgct cacccactcg cgaatggcct ggcccacggg ggtcaccggc
     2881 gccggcgcat cgttcgccag ctcggcgggg cggttccgct gcgagtcgag gatgttggtg
     2941 tcgacccacc ccggacacag caccgagatc ttgggcttca ggccgccgcg gatcagctcg
     3001 aggttcacgc tctcggacag cccgacgacc gcaaacttcg tcgtgcaata cagcgtcatc
     3061 acgctgggta ccagacccgc caaggaggcg gtattcacga tgtgcgcctc ggtgtcttgc
     3121 ttgatcatga tgggcagaaa cgtgcggatg ccatgaacga ctcccatcag attgacgccg
     3181 agtatccatt gccaatcctt caagctgctg gtccagctct gtccgccacc cgagccgccc
     3241 acgccggcgt tgttgcacag gacatggacc gcgccgtacg tgcgcagcgt ctgttcggca
     3301 agctgctcga cctgctccgg ccgggacaca tcggtcgcga ccgcgtgaac atccgcgccg
     3361 ctcgcgcgca gcgccgagca tgtccgttcg agcgcaggct gttcgatgtc gctcagcacg
     3421 accttcatcc cggcatcggc gaatacctcc gccatgccgc gaccgatgcc gctggcggcg
     3481 ccggtcacca ccgctacttt gtccttgacg tccttcatgg ttgcccctcg aattcgttgg
     3541 ctcgtcgaat cccgccgcat cgacaggagc gacacatcct ggccctcgac acgatgtgtt
     3601 atcgccggtc cttgcaaaac atgcatctac caaataggat cggaggcgat tcgagtacgc
     3661 cgctctatgg ggaatacccg cccgtggtgc cgtggatcgt tctccagctc gccgactcgg
     3721 cggtcccgat cggcgggttc gcgcactccg gcggcctgga ggcgctggtc gccgcgcgcg
     3781 agctcggtgc gctcgaggcg ttctgccgcg cgctgatcga ccagcaggcg cacggcgcgc
     3841 ggccgctggt cggcgccgcg tgggacgcgc cggaccggct cgccgagctc gacgctcgca
     3901 gccgcgcgat cctgt
//
LOCUS       DASVOR010002311         2081 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_200693,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002311 DASVOR010000000
VERSION     DASVOR010002311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200693"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2081
                     /locus_tag="VF516_42650"
     CDS             <1..>2081
                     /locus_tag="VF516_42650"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEX8114527.1"
                     /translation="GHRAVPMGEDTALDVIPVDFIASGMLLATAAVLAGEHEPVYQLG
                     TSDSNRVTSRRLTELTALAVRRLYRDKAERGEDELRSRLRARLEGMPVTYDHFERWSA
                     PMFKRIADRLIRVIDDQLPRWGAPRLEAFAERARDELTKISTFTGQVQDLIELFKPFT
                     TDHDLSFRCDHIRALWARVTLADQDRLLWATHLIDWRKYWLDTHFPGLQKWTFDKLDE
                     EFGAKPKTVYTHKELVELFEATVKLHKNRIALRLVRKDENAEPVAYTYGQLGKMAWQG
                     AATLRQIGVGAGDRVVLMAENRPEWGIAYFAILLAGATAVPLDRELSLPEVVNLARVS
                     RARALVLSRKVVERLAGEANVAVPLGDGDQDAVWSPAHPAFGNWLAQRPEGEAIHARV
                     FAFDELLTEPDVVFVDPLRPSFSSSAAAPAPYPDVKGDTLASLIFTSGTTGTPKGVML
                     THKNLTSMVSKLSSLFTLYKHDRLLSVLPLHHTLEFSAGFLMPLLHGASVDYLEEIDA
                     DTLARALEDQGITGMVGVPALFQLLERKIYKNVSDAGVLVEKAFDSIVDLHRSLRDKV
                     PWDISAGKLLFYPVHRKLGGRIRLLITGGSALPPDTLKTFRGLGFNLYEGYGMTESSP
                     VLTVTRPGDKVIPGSVGRALPGVDVRIDHPDASGVGEVIAKGPNVMTGYFENAEATAQ
                     TLQDGWLHTGD"
BASE COUNT          314 a          736 c          725 g          306 t
ORIGIN      
        1 gggccaccgc gcggtgccga tgggcgagga caccgcgctc gacgtgatcc cggtcgactt
       61 catcgccagc ggcatgctgc tggcgaccgc cgcggtgctc gccggcgagc acgagccggt
      121 ctaccagctc ggcaccagcg actccaaccg ggtgacctcg cggcggctga ccgagctgac
      181 cgcgctggcg gtgcgccggc tgtaccgcga caaggccgag cgcggcgagg acgagctgcg
      241 ctcgcggctg cgcgcgcggc tcgagggcat gccggtgacc tacgaccact tcgagcgctg
      301 gtcggcgccg atgttcaagc ggatcgccga ccggctgatc cgcgtgatcg acgaccagct
      361 gccgcgctgg ggcgcgccgc ggctcgaggc gttcgccgag cgcgcgcgcg acgagctcac
      421 caagatctcg acgttcaccg gccaggtcca ggacctgatc gagctgttca agccgttcac
      481 caccgaccac gacctcagct tccggtgcga ccacatccgc gcgctgtggg cgcgcgtgac
      541 gctggccgat caggaccgcc tgctgtgggc gacgcacctg atcgactggc gcaagtactg
      601 gctcgacacc cacttccccg gcctgcagaa gtggacgttc gacaagctcg acgaggagtt
      661 cggcgccaag cccaagaccg tctacaccca caaggagctg gtcgagctgt tcgaggccac
      721 ggtcaagctg cacaagaacc ggatcgcgct gcggctggtc cgcaaggacg agaacgccga
      781 gccggtcgcg tacacctacg ggcagctcgg caagatggcg tggcagggcg cggccacgct
      841 gcgccagatc ggggtcggcg cgggcgaccg cgtggtcctg atggcggaga accgcccgga
      901 gtggggcatc gcgtacttcg cgatcctgct cgccggcgcg accgcggtgc cgctcgatcg
      961 cgagctgtcg ctccccgagg tcgtgaacct cgcgcgggtg tcgcgcgccc gtgcgctggt
     1021 gctgtcgcgc aaggtggtcg agcggctcgc cggcgaggcg aacgttgccg tgccgctcgg
     1081 cgatggcgac caggacgccg tgtggtcgcc ggcgcacccg gcgttcggca actggctggc
     1141 gcagcgtccg gagggcgagg cgatccacgc gcgcgtgttc gcgttcgacg agctgttgac
     1201 cgagcccgac gtcgtgttcg tcgatccgct gcggccgtcg ttctcgtctt cggcggcggc
     1261 cccggcgccg taccccgatg tcaagggcga cacgctggcg tcgctgatct tcacctcggg
     1321 gaccaccggc acgcccaagg gcgtgatgct gacccacaag aacctgacct cgatggtcag
     1381 caagctgtcg tcgctgttca cgctgtacaa gcacgatcgg ctgctgagcg tgttgccgct
     1441 gcaccacacg ctggagttct cggccgggtt cctgatgccg ctcctgcacg gcgcctcggt
     1501 cgattacctc gaggagatcg acgccgacac gctggcccgc gcgctcgagg accagggcat
     1561 caccggcatg gtcggcgtcc cggcgctgtt ccagctgctg gagcgcaaga tctacaagaa
     1621 cgtcagcgac gccggcgtgc tggtcgagaa ggcgttcgac tcgatcgtcg acctccaccg
     1681 atcgctgcgc gacaaggtgc cgtgggacat cagcgccggc aagctgctgt tctatccggt
     1741 gcaccgaaag ctcggcggcc ggatccgcct gctcatcacc ggcggctcgg cgctgccgcc
     1801 cgacacgctc aagacgttcc gcggcctggg cttcaacctc tacgagggct acggcatgac
     1861 cgagtcgtcg ccggtgctca ccgtgacccg gcccggcgac aaggtcatcc ccggctcggt
     1921 cggccgcgcg ctgcccggcg tcgacgtccg gatcgatcac cccgacgcca gcggggtcgg
     1981 cgaggtgatc gccaagggcc ccaacgtgat gaccggctac ttcgagaacg ccgaggcgac
     2041 cgcgcagacc ctgcaggacg gctggctgca caccggcgat c
//
LOCUS       DASVOR010002312         2752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_229847,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002312 DASVOR010000000
VERSION     DASVOR010002312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229847"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VF516_42655"
     CDS             complement(<1..256)
                     /locus_tag="VF516_42655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114528.1"
                     /translation="MPPEPDRVPRDKVNDYTEAEAARRRAFARDHTGVELRDVGVYSV
                     PPGSVAPAPVPRAARLLRLGQGRQARRDLRGGGARRRHLAG"
     gene            complement(256..2043)
                     /locus_tag="VF516_42660"
     CDS             complement(256..2043)
                     /locus_tag="VF516_42660"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEX8114529.1"
                     /translation="MEHIHNLVELYQRACAQHADREILGTRKDGKWQWLTYRQFRGLV
                     DDARAGLAGLGIEPGDRIAFIGDNSVEWAVAAYATYGLAATFVPMYQAQRPSEWQFIL
                     KDCGARAVIVTNEATYDAIGGMRANLPELRHVIGVARPDGAPGSWRDLLAAGAARPVP
                     ARSPDPQSVAGFLYTSGTTGKPKGARLSHRNITSNIEAIHQLYDFSPSDRYLSFLPWA
                     HAYGQTVELHMMFSWGTSLAINDELPRLLANMTEVKPTVLVAVPRIFNRIYDAVTREL
                     AARPELVQKIVQHGLVQALKRTRGEPMTALDKLELALDDRLIFQKIRDKFGGRLRYAF
                     TGSASIARHVMEFIDALGIEVHEGYGLTETSPVVTTNSPGHRKFGSVGRVLPGVRVEV
                     DTSVTDDHQSGEIIVYGPNVMLGYHNRPEDNAAALMPDGGLRTGDLGYLDADGYLYIT
                     GRIKEQYKLENGKYVMPSALEETLKLSPFIANVMIHGDGRPYNVALVVPDAQAVRGWA
                     QKQGLTLPEDMCTDDRVRELVVSEIERLSGDFKGFEKPRAVALVGEDFTIGNGLLTPT
                     LKLKRREVLARHGKIIDDLYARTPVKELA"
     gene            complement(2098..>2752)
                     /locus_tag="VF516_42665"
     CDS             complement(2098..>2752)
                     /locus_tag="VF516_42665"
                     /inference="COORDINATES: protein motif:HMM:NF019436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114530.1"
                     /translation="LAPRGDGHPVLVLPGLAAGDDSTAILRGFLRSLGYAARGWGQGC
                     NLGFRSGVLDALRHRILDLAGEHGHKVSLIGWSLGGIFARELAKEVPDSVRLVITLAS
                     PFTGHPRATHAFRLYEWMSGHRIGAPEVHEPLRAPPPVPTTSIWSRTDGVVSWRCSVE
                     RELPLVENIEVNASHLGIGAHPLALYAIADRLAQPEGVWRRFQREGWKALAYGDARH"
BASE COUNT          382 a          944 c          988 g          438 t
ORIGIN      
        1 cgccagcgag atgtcgccgg cgagcgccac cgccgcgcag atctcggcga gcttgtcggc
       61 cttgcccgag ccgaagcagc cgagcagctc gaggcactgg cgctggggcg acgctgcccg
      121 gcgggaccga gtacacgccg acgtcgcgca gctcgacgcc ggtgtggtcg cgggcgaacg
      181 cgcgccgccg ggcggcctcg gcctcggtgt agtcgttgac cttgtcgcgc gggacgcgat
      241 ccggttcggg gggcatcacg cgagctcctt gaccggggtt cgcgcgtaca gatcgtcgat
      301 gatcttgccg tggcgggcga gcacctcgcg gcgcttcagc ttgagggtcg gggtcaagag
      361 gccattgccg atcgtgaagt cctcaccgac cagcgcgacc gcgcgcggct tctcgaagcc
      421 cttgaagtcc ccggacagcc gctcgatctc ggacaccacg agctcgcgca cgcggtcgtc
      481 ggtgcacatg tcctcgggca gggtcagccc ctgcttctgc gcccagcccc gcaccgcttg
      541 cgcatccggg acgaccagcg cgacgttgta cggccggccg tcgccgtgga tcatcacgtt
      601 ggcgatgaac ggcgacagct tgagcgtctc ctcgagggcc gacggcatga cgtacttgcc
      661 gttctccagc ttgtactgct ccttgatccg gcccgtgatg tagagatagc cgtcggcgtc
      721 gaggtagccg aggtcgccgg tgcgcagccc gccgtcgggc atcagcgcgg cggcgttgtc
      781 ctcgggccgg ttgtgatacc cgagcatcac gttggggccg tagacgatga tctcgccgga
      841 ctggtgatcg tcggtgacgc tggtgtcgac ctcgacccgc acgccgggca gcacgcggcc
      901 gacgctgccg aacttgcggt ggccgggcga gttggtggtg accaccgggc tggtctcggt
      961 cagcccgtag ccctcgtgga cctcgatccc gagcgcgtcg atgaactcca tgacgtggcg
     1021 cgcgatcgac gcgctgccgg tgaacgcgta gcgcagccgg ccgccgaact tgtcgcggat
     1081 cttctggaag atcagccggt cgtcgagcgc gagctcgagc ttgtcgagcg cggtcatcgg
     1141 ctcgccccgc gtgcgcttga gcgcctgcac gaggccgtgc tgcacgatct tctggaccag
     1201 ctcgggccgc gccgcgagct cgcgggtcac ggcgtcgtag atccggttga agatccgcgg
     1261 aacggcgacc agcaccgtcg gcttgacctc ggtcatgttg gcgagcagcc tcggcagctc
     1321 gtcgttgatc gccagcgacg tgccccagct gaacatcatg tggagctcga cggtctggcc
     1381 gtacgcgtgc gcccagggca ggaacgacag gtagcgatcg ctgggcgaga agtcatagag
     1441 ctgatggatc gcctcgatgt tcgacgtgat gttgcggtgg ctcagccgcg cccccttggg
     1501 cttgccggtg gtgcccgagg tgtagaggaa gccggcgacc gactgcggat cgggcgatcg
     1561 cgccgggacc ggccgggccg cgccggcggc gagcaggtcg cgccacgatc ccggggcgcc
     1621 gtcgggccgg gccacgccga tcacgtggcg gagctcgggc aggttggcac gcattccgcc
     1681 gatcgcgtcg taggtcgcct cgttggtgac gatcacggcg cgcgcgccgc aatccttgag
     1741 aatgaattgc cattcgctgg gtcgctgcgc ctggtacatc ggcacgaacg tcgccgccag
     1801 gccgtaggtc gcgtacgcgg cgaccgccca ctcgacgctg ttgtcgccga tgaacgcgat
     1861 ccgatcgccg ggctcgatgc cgaggccggc gagcccggcg cgggcgtcat cgaccaggcc
     1921 gcggaactgc cggtaggtca gccattgcca cttgccatcc ttcctggttc cgaggatctc
     1981 gcggtcggca tgttgcgcgc aggctcgctg atacagctct acgaggttgt ggatgtgctc
     2041 cattggcgga ccttcctttc ttgcccccgc atgcacgcat ttcctgtgcc ggccacgtca
     2101 gtgccgcgcg tcgccgtacg ccagggcctt ccagccctcg cgctggaacc gccgccacac
     2161 gccctcgggc tgcgccagcc ggtcggcgat cgcgtacagc gccagcggat gggcaccgat
     2221 cccgagatgg ctggcgttga cctcgatgtt ctcgaccagc ggcagctcgc gctcgacgct
     2281 gcaccgccac gacaccacgc cgtcggtccg gctccagatc gacgtggtcg gcaccggcgg
     2341 tggtgcgcgc agcggctcgt ggacctcggg cgcgccgatc cggtggcccg acatccactc
     2401 gtagagccgg aacgcatggg tcgcgcgggg atggccggtg aacggcgacg ccagcgtgat
     2461 cacgaggcgc accgagtccg ggacctcctt ggcgagctcg cgtgcgaaga tcccgccgag
     2521 gctccagccg atcaggctga ccttgtggcc gtgctcgccg gcgagatcga ggatccggtg
     2581 gcgcagcgca tcgagcacgc cggagcggaa gccgaggttg cagccctggc cccagccgcg
     2641 cgcggcgtag ccgaggctgc gcaggaatcc gcgcaggatc gccgtgctgt cgtcgccggc
     2701 ggccaggccg ggcagcacga gcaccgggtg gccgtcgccg cgcggcgcca gg
//
LOCUS       DASVOR010002313         3011 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_463383,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002313 DASVOR010000000
VERSION     DASVOR010002313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3011
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463383"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..498)
                     /locus_tag="VF516_42670"
     CDS             complement(<1..498)
                     /locus_tag="VF516_42670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114531.1"
                     /translation="MRGLILSAAFGAAALTGCYVQDTAGPSYSATATVSSDGYAATPD
                     LVTVQPGVQVVADYDEPVFFVDGFYWRFYDGYWYRSNSYYAGWYFYERPPVAVLRIDR
                     PYAYAHYRPAGYVGRASVRYRRPEVITRDHRAVQTAPAYQARPGYAAPATVDHRTVAP
                     APAPAV"
     gene            complement(741..1028)
                     /locus_tag="VF516_42675"
     CDS             complement(741..1028)
                     /locus_tag="VF516_42675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114532.1"
                     /translation="MSKPTQELQTIDPAALAQVSGGTTATTSSATNDQILTALNGILS
                     SIQGLAQPSSGGLNQQEMLMLMMIMQQRNQQQVIATGNPWGGWGQPPIYYY"
     gene            complement(1190..2311)
                     /locus_tag="VF516_42680"
     CDS             complement(1190..2311)
                     /locus_tag="VF516_42680"
                     /inference="COORDINATES: protein motif:HMM:NF014090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HEX8114533.1"
                     /translation="MADDDLRRFRANRQGEVDSAAQYRAMADAEPDTGVARIYRELAD
                     VEDKHAAFWETRMRERGVEPGPRRASWRARVLSWTARRLGPRTILPTVAQSEAVGRND
                     YVGQSETAHTRMPAQERSHARVLKALLKRPEGVAGNELARVEGRHRNVGGNALRAAVL
                     GANDGLCSNLALVTGVAGASTSSHAILVTGIAGLLAGACSMALGEWVSVTSSRELAER
                     ELHIEKEELAAAPEEEREELQLIYEAKGIPKDEARQLSQRLLDDHGAALDVLAREELG
                     IDPEELGGSARVAAATSFLLFAFGAMFPVLPYLFATGTTAVIASLVVSSLALFGIGAA
                     ITLFTGRSVWRSGGRQTVLGLAAAGLTFGLGHLLGTALG"
     gene            complement(2338..>3011)
                     /locus_tag="VF516_42685"
     CDS             complement(2338..>3011)
                     /locus_tag="VF516_42685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8114534.1"
                     /translation="AGDAMGSPRFFTRDYAEHAALRDGTRVVLRLIRSEDRELLRAGF
                     DRLSPASRYSRFLAPKASLSDDELRYLCDVDQENHVAIGAVREDDPAFGLGIARFIRQ
                     PDRPDTAEAAIAVADEVQHQGLGSLLGLRLIAAALERGVTRFRCEVLGSNAGMTGLLD
                     QIAPDRTVELGSGVMSVELELPPVSPTQSPAGEPRETAVYRLLRAAAQNAVEWTEAVR
                     KLWRR"
BASE COUNT          410 a         1041 c         1146 g          414 t
ORIGIN      
        1 caccgcgggc gcgggagccg gggcgaccgt gcggtgatcg acggtcgcgg gcgccgcgta
       61 gccgggcctg gcctgatacg ccggagccgt ctggacggcg cgatggtcgc gcgtgatcac
      121 ctcggggcgg cggtagcgga cgctggcccg cccgacgtac ccggccgggc ggtagtgagc
      181 gtaggcgtac ggccggtcga tccgcagcac cgcgaccggc ggccgctcgt agaagtacca
      241 gccggcgtag tagctgttcg agcggtacca gtagccgtcg tagaaccgcc agtagaagcc
      301 gtcgacgaag aacaccggct cgtcgtagtc ggccacgacc tgcacgcccg gctggaccgt
      361 caccaggtcc ggcgtggccg cgtagccgtc gctgctcacg gtcgcggtgg cgctgtagct
      421 cgggcccgcg gtgtcctgga cgtagcagcc ggtgagcgcc gctgctccga acgcagccga
      481 taggatcagt ccacgcatgt cattcctccg atgatcggca agttgagcat gccgcatgcc
      541 agcgtcaatg acctgtgaaa tcaacggtta ccatcgcgag gcgtgagcgc cggtgcgcac
      601 tcctggcgtg gcgacaacgt cacagggcgg aaaaatgcgg acatacgcgg ctcgcgctcc
      661 cgggcggcgg tccgggagcg cggatcgagc gtccggccgc gcggatcgcg gtccgtgcgg
      721 aggagcgatg tacgggcagc ctagtagtag tagatcggcg gctgacccca gcccccccac
      781 gggttgcccg tggcgatgac ctgctgctgg ttgcgctgct gcatgatcat catcagcatc
      841 agcatctcct gctgattgag tccgccgctg ctgggctgcg cgaggccctg gatcgacgag
      901 aggatcccgt tgagcgcggt gaggatctgg tcgttggtcg cggaagacgt cgtggcggtg
      961 gtgccgccgc tgacctgggc gagggcagcg gggtcgatgg tctggagctc ctgggtgggc
     1021 ttcgacatgg tggtgatgtc ctttgtcggc tacgtcgagc cctcgttaca gcagccggtg
     1081 tgccaccggc gagggtccag cgatcttcgc cggatccggc gactcccggg ggcggcgggg
     1141 cgggtcgacg cctaccgcgg gatgcaaccg ggcgaggcga tccccacggt caacccaggg
     1201 cggtccccag caggtggccg agcccgaagg tcaggccggc cgcggcgagg ccgagcacgg
     1261 tctggcggcc gcccgagcgc cacaccgacc gcccggtgaa cagcgtgatc gccgcgccga
     1321 tcccgaacag cgcgaggctc gagacgacca ggctggcgat caccgcggtc gtcccggtcg
     1381 cgaacaggta gggcaggacc gggaacatcg cgccgaacgc gaacagcagg aacgaggtgg
     1441 ccgcggcgac ccgggccgag ccgccgagct cctcggggtc gatgccgagc tcctcgcggg
     1501 cgagcacgtc gagcgcggcg ccgtggtcgt cgagcaggcg ctgcgacagc tggcgggcct
     1561 cgtccttggg gatgcccttg gcctcgtaga tcagctggag ctcctcgcgc tcctcctcgg
     1621 gggcggcggc gagctcctcc ttctcgatgt ggagctcgcg ctcggcgagc tcgcgcgacg
     1681 aggtcaccga cacccactcg ccgagcgcca tcgagcacgc gccggcgagc aggccggcga
     1741 tcccggtgac caggatcgcg tgcgagctcg tggacgcgcc ggcgacgccg gtgaccagcg
     1801 cgaggttcga gcacaggccg tcgttggcgc cgagcaccgc ggcgcgcagc gcgttgccgc
     1861 cgacgttgcg gtggcggccc tcgacgcggg cgagctcgtt gccggcgacg ccctcgggcc
     1921 gcttcagcag ggccttgagc acgcgcgcgt gcgagcgctc ctgggccggc atccgggtgt
     1981 gcgcggtctc ggactggccg acgtagtcgt tgcggcccac ggcctcgctc tgcgcgacgg
     2041 tcggcaggat cgtgcgcggg ccgagccgcc gcgcggtcca cgacagcacg cgggcgcgcc
     2101 agctcgcgcg ccgcggtccg ggctcgacgc cgcgctcgcg catccgggtc tcccagaacg
     2161 cggcgtgctt gtcctcgacg tcggcgagct cgcggtagat ccgggcgacg ccggtgtcgg
     2221 gctcggcgtc ggccatcgcg cggtactgcg cggcgctgtc gacctcgccc tggcggttgg
     2281 cgcggaaccg gcgcagatcg tcgtcggcca tgctgcttgg taccacaggg ccgggcgtca
     2341 gcgccgccac agcttgcgca cggcctcggt ccactcgacc gcgttctggg cggcggctcg
     2401 cagcagccgg tagaccgcgg tctcgcgcgg ctcgccggcg ggcgactggg tcggcgacac
     2461 cgggggcagc tcgagctcga cggacatcac tccggatccg agctcgacgg tccggtcggg
     2521 agcgatctga tcgagcaggc cggtcatgcc ggcgttcgag cccagcacct cgcagcggaa
     2581 ccgggtgacg ccgcgctcga gcgcggccgc gatcaaccgg aggccgagca ggctgcccag
     2641 gccctggtgc tggacctcgt cggcgaccgc gatcgccgcc tcggcggtgt cgggccggtc
     2701 gggctggcgg atgaaccgtg cgatgcccag gccgaacgcc gggtcgtcct cgcgcaccgc
     2761 gccgatcgcg acgtggttct cctggtcgac gtcgcacagg tagcgcagct cgtcgtcgga
     2821 cagcgacgcc ttgggggcga ggaaccgcga gtagcgcgac gccggcgaca gccggtcgaa
     2881 gccggcgcgc agcagctcgc ggtcctcgga ccggatcagc cgcagcacga cccgggtgcc
     2941 gtcgcgcagc gcggcgtgct ccgcgtagtc gcgggtgaag aaccgcggcg aacccatggc
     3001 gtccccagca t
//
LOCUS       DASVOR010002314         2136 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_463420,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002314 DASVOR010000000
VERSION     DASVOR010002314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463420"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VF516_42690"
     CDS             <1..1292
                     /locus_tag="VF516_42690"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8114535.1"
                     /translation="IGTDDVFELYATKEDPEEAKAKRFYNNTRFVRVTAGAQYHDGPW
                     SAKLAVSGIAQQFIFEAGLYQQIHVTTPAVTPRVEVTRTADSWAGLSKIEWRTGAEAQ
                     VGRSSLDLALPLERREGQPMPPRNPKDTSVKFNGSFWIPDFAAWTLLSANLDPAIRAT
                     VGVRSDAFARVDEVNLEPRGEIQIKLPQHLTARLSAGAYRRPPEFQTELLHTEVGSEH
                     SAQTIAGLQYEPREGIRVQTSAYYTDRSHLIKANPDGTLNNDGRGTTVGAELLATYRD
                     GAWFGWLSYSYSHSTRVDEPGIASRLFDYDQPHSLNAALSWKGRRWQLGGRFQLYSGL
                     PYTPPIGAVFESDKNLYVPVYGAVNTERVPMHHQLDLRVDYFWRWGPTEMTAFLDVQN
                     VYMNESIARYFYSYDYSQRQPFKSLPIIPSIGLRGVL"
     gene            1289..1993
                     /locus_tag="VF516_42695"
     CDS             1289..1993
                     /locus_tag="VF516_42695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114536.1"
                     /translation="MIRAPGTVIGFAAIAAAATGCMGDIDPPWQLSHDRIVAVRADPP
                     GITAGQTSRIDALLAHKGQMTSVATPELAAVTSPMSLSDVLSIRGGTWTVTAPGEDRL
                     AAARGELMLPAGAPVPLQIGVSYGGQTLIATKTITLGQPAANPTLDGLMIDGKPPGDP
                     LTVGKLVNVPLSINASDADFDVTWLTSCGTMHDFDLPQAYLRVETADPQIGELGVVLR
                     DAQGGVAWKTWPIHAE"
BASE COUNT          326 a          828 c          691 g          291 t
ORIGIN      
        1 cgatcggcac cgacgacgtg ttcgagctct acgcgaccaa ggaggacccc gaggaggcca
       61 aggccaagcg gttctacaac aacacgcggt tcgtgcgggt caccgccggc gcccagtacc
      121 acgacggccc gtggtcggcc aagctcgcgg tgtccggcat cgcgcagcag ttcatcttcg
      181 aggccggcct gtaccagcag atccacgtca ccacgcccgc ggtcacgccg cgcgtcgagg
      241 tcacccgcac cgccgatagc tgggccgggc tgtcgaagat cgagtggcgg accggcgccg
      301 aggcccaggt cgggcggtcg tcgctcgacc tcgcgctgcc gctcgagcgc cgcgagggcc
      361 agccgatgcc gccgcgcaat cccaaggaca cctcggtcaa gttcaacggc tcgttctgga
      421 tccccgactt cgcggcgtgg accctgctgt cggccaacct cgatcccgcg atccgcgcga
      481 cggtcggggt gcggtccgac gcgttcgccc gggtcgacga ggtcaacctc gagccgcgcg
      541 gcgagatcca gatcaagctg ccgcagcacc tcaccgcccg gctgtccgcc ggtgcgtacc
      601 gccggccgcc ggagtttcag accgagctcc tgcacaccga ggtcggctcc gagcactcgg
      661 cccagaccat cgccggcctg cagtacgagc cgcgcgaggg catccgggtc cagacctcgg
      721 cctactacac cgaccgctcg cacctcatca aggccaaccc cgacggcacg ctcaacaacg
      781 acggccgcgg caccacggtc ggcgccgagc tgctcgcgac ctaccgcgac ggcgcgtggt
      841 tcggctggct cagctactcg tactcgcact cgacgcgggt cgacgagccg ggcatcgcga
      901 gccggctgtt cgactacgac cagccgcaca gcctcaacgc cgcgctgagc tggaagggcc
      961 ggcgctggca gctcggcggc cggttccagc tgtactccgg cctgccctac acgccgccga
     1021 tcggcgcggt gttcgagagc gacaagaacc tctacgtccc ggtctacggc gcggtgaaca
     1081 ccgagcgcgt cccgatgcac caccagctcg acctccgggt cgattacttc tggcgctggg
     1141 gtcccaccga gatgaccgcg ttcctcgacg tccagaacgt ctacatgaac gagtcgatcg
     1201 cgcgctactt ctacagctac gactactccc agcgccagcc gttcaagtcg ttaccgatca
     1261 tcccgtcgat cggcctgcga ggtgtcctgt gatccgggcg ccggggaccg tgatcgggtt
     1321 cgcggcgatc gccgccgccg cgaccggctg catgggcgac atcgatccgc cgtggcagct
     1381 cagccacgac cgcatcgtcg cggtgcgcgc cgacccgccc ggcatcacgg caggccagac
     1441 ctcgcggatc gacgcgctgc tcgcccacaa gggccagatg accagcgtgg cgacccccga
     1501 gctggccgcc gtgacctcgc cgatgagcct gtccgacgtg ctgtcgatcc gcggcggcac
     1561 ctggacggtc accgcgcccg gcgaggaccg cctggccgcc gcgcgcggcg agctcatgct
     1621 ccccgccggc gccccggtgc cgctgcagat cggcgtctcg tacggcggcc agacgctgat
     1681 cgcgaccaag acgatcacgc tcggccagcc cgccgccaac ccgacgctcg acggcctgat
     1741 gatcgacggc aagccgcccg gcgacccgct caccgtcggc aagctggtca acgtgccgct
     1801 ctcgatcaac gccagcgacg ccgacttcga tgtcacctgg ctgacctcgt gcggcaccat
     1861 gcacgacttc gacctgcccc aggcctacct gcgggtcgag accgccgatc cgcagatcgg
     1921 cgagctcggc gtcgtgctgc gcgacgcgca gggcggcgtg gcctggaaga cctggccgat
     1981 ccacgccgag tagcgccggc gcgctcgccg cgtcatcgta tcgtcgtggc gatccgcctc
     2041 gtcctcgggc tcgcactgct cgccggcctg gccgccggct gcggcgcgaa ctgcgccgag
     2101 atcgccgccc gcaagcgcgc gctgaccgag cgcacc
//
LOCUS       DASVOR010002315         3528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_521605,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002315 DASVOR010000000
VERSION     DASVOR010002315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3528
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521605"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="VF516_42700"
     CDS             <1..759
                     /locus_tag="VF516_42700"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEX8114537.1"
                     /translation="PASAPVEVGLNPGESMAFEVRLAGLVAGEAQLAVGQLGDYEGHR
                     AVVVKSRAATAGAAAMIRHIVDEATTVIDLDTGRPLALDTVVEQGDNRTTASAKFTDN
                     VADITYVRSDEHEPHHLRVDFGKGTAAAAGIQVHDAHSAMAQLRGWRAAPGTRRTVFV
                     VGGRRLWRIDVTYAGQDTVGSALGNRRAIRFFGESYRARPDFSPETPRPTRTFTVWLS
                     DDADRVPLKVAARTELGEVMMNLTEYNRNGAPAE"
     gene            complement(1282..2046)
                     /locus_tag="VF516_42705"
     CDS             complement(1282..2046)
                     /locus_tag="VF516_42705"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8114538.1"
                     /translation="MTTARPASHRARPRRSEPEQLVLWKKRGGKRRGAGRPPKGPRAG
                     SPHKQRPYLHARYPVHVTLRVVGAVGSLRRRCVYQAIREATLTTALREDFRIVHLSIQ
                     RTHVHLLVEANDKAALARGMQGFQVSAAKHLNAAISKGTPGSRRRGTVFPDRYHAAII
                     TSPRQARHALSYCLNNWRRHQEDRKAPMSGWTIDWFSSAAMFPGWAEYGDQPFLWRGP
                     ATYDPLVVYQPRTWLLREGWRKAGTISCREVPVAGR"
     gene            complement(2493..>3528)
                     /locus_tag="VF516_42710"
     CDS             complement(2493..>3528)
                     /locus_tag="VF516_42710"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEX8114539.1"
                     /translation="LADQARRARSALGLPDRARLVRGWALLIGATLVVAPAAYRIAAV
                     LQRPTGGTARQAGGLTPPTDAGTAGTVDAAANAVGSRCPPDMVAVLAGTFRMGSPAGT
                     GDDDEHPGHDVTLSAYCIDRTEVTVKAYAACVQAKGCTAAPLTVQWSGGSAQDVQRYS
                     RFCNREDRPDHPINCVDWNQAAAYCTWADKRLPSEAEWEYAARGTDGRVYPWGNETPS
                     ATRLNACGPECVAMAKRELNADWRPMYDTNDAWESTAPVGRFPAGASPFGALDMAGNV
                     WEWTADWYGTYKATAATNPRGAEMGTERVIRGGGWYNLVAAVVGAAYRYRYEPSHRRH
                     GVGFRCARGD"
BASE COUNT          562 a         1261 c         1167 g          538 t
ORIGIN      
        1 cccgcctccg cgccggtcga ggtcggcctc aacccgggcg agtccatggc gttcgaggtc
       61 cggctcgccg gcctggtcgc gggcgaggcc cagctcgcgg tcggccagct cggcgactac
      121 gagggccacc gcgccgtcgt cgtcaagtcg cgcgccgcca ccgcgggcgc cgccgcgatg
      181 atccgccaca tcgtcgacga ggcgaccacc gtgatcgacc tcgacaccgg ccggccgctc
      241 gcgctcgaca ccgtggtcga gcaaggcgac aaccggacca ccgcgagcgc gaagttcacc
      301 gacaacgtcg ccgacatcac ctacgtccgg tccgacgagc acgagccgca ccacctgcgg
      361 gtcgacttcg gcaagggcac ggcggccgcc gcgggcatcc aggtccacga cgcgcactcg
      421 gcgatggccc agctgcgcgg ctggcgcgcc gcgccgggca cgcgtcgcac ggtgttcgtc
      481 gtcggcggcc gccggctgtg gcggatcgac gtcacctacg ccggccagga caccgtcggc
      541 tcggcgctcg gcaaccggcg cgcgatccgg ttcttcggcg agtcctaccg cgcccggccc
      601 gacttctcgc ccgagacccc gcgcccgacc cgcacgttca cggtgtggct cagcgacgac
      661 gccgatcgcg tcccgctcaa ggtcgccgcc aggaccgagc tcggcgaggt catgatgaac
      721 ctgaccgagt acaaccgcaa cggcgccccg gccgagtagc catcggttca aggaagggtg
      781 gaaggggcga ttcaagacgc atgctttcgt tgatgttgac ggtttgacgt cgacgatgac
      841 cctcgaacgt tgaggttgag ggcgacgtac aacgtcgact ccaccgtcga cctcgacgtc
      901 ggcttcagat cctcgaagga agctccgcga tcacctcgaa cgcgtgggcg aagaactcga
      961 tggagcgccg atcgacctcg agacgctgaa agctgagcat gccacaaggt aggctgttct
     1021 cacctgtatc ggctgcgccg tccgacggtt ggttcatacc ggagtcaacg tgcaaggccc
     1081 agggtggggt cagaactcta cgttgccgtc gacctcaagg gtggagtcaa ggtcaaggtc
     1141 caggtccacg tccaggcgtc gatgacggct acgcgagcaa catcgtggcg tgtgccggca
     1201 cattcgacgc gatggccgcg agcagcgcga cgttcggcct ctgtactggc tgccactcga
     1261 tcctgagtcg acggcctccg gtcatcgtcc cgccacgggc acctcgcggc atgagatcgt
     1321 gccagccttc cgccaaccct ccctcagcag ccatgtcctg ggttgataca cgacgagtgg
     1381 gtcgtaggtc gcagggccac gccacaggaa tggctggtcg ccatactccg cccagcccgg
     1441 aaacatcgcc gcgctggaga accagtcgat cgtccagccg gacatcggcg ccttgcggtc
     1501 ctcctggtgg cgacgccagt tgttcaagca gtaggacaga gcgtgccgcg cctgacgcgg
     1561 cgacgtgatg atcgccgcgt ggtagcggtc aggaaacacc gtgccccgcc ggcgcgaccc
     1621 gggcgtgccc ttgctgatcg ccgcgttcag gtgcttggcg gcggagacct gaaagccctg
     1681 catccccctc gccagcgcag ccttgtcgtt ggcctcgacg agcaagtgga cgtgcgtgcg
     1741 ctggatgctg aggtggacga tgcggaagtc ctcgcgcagc gcggtcgtga gcgtggcttc
     1801 gcgaatcgcc tggtacacac accggcggcg caggctgccg accgcgccga cgacacgcag
     1861 cgtgacatga acgggatacc gcgcatgaag atacggccgc tgcttgtgcg gcgatccggc
     1921 ccgcgggccc ttcggcggcc gccccgcccc acgccgcttg ccgccgcgtt tcttccacag
     1981 gacgagctgc tcgggctcgg agcgccgcgg acgggctcga tgcgaggcgg gtcttgcggt
     2041 ggtcatgcag tctggttagc caatgaacct cgatcacgca atcgatttcg ccgagatttt
     2101 tgtggtaaac agtggcagag tcgtcttctc ggatcacact ggcggtgacc acgcggttcc
     2161 tgctcgagct cgagcccgcg ctgtgcgcgc tgcgggaaga tcttctcgcg aacacgtggc
     2221 ggcccggaat gccgcgactg gccctccgtg atcccaagcc tcggaccatc accatcgcac
     2281 cgttccggga tcgcgtggtc caccaggcgc tgtcgatcgt gatcgcgcca cgcatcgagc
     2341 gccgcctggt cggcgacagc tacgcgtaca ggcccggacg cgtcagccac gcggcgctca
     2401 cacgtggccg cgcatgggcg cgtgggcggg cggctccggg gagaacagag gacctgaaga
     2461 agaacaagac ggaagaacag aaaagcgaag actcaatccc cgcgtgcaca acggaacccg
     2521 acgccgtggc gacggtgcga cggttcatac ctgtagcgat acgcggcgcc aacgacggcg
     2581 gcgacaagat tgtaccaacc gccgccgcgg atcacgcgct ccgtgcccat ctccgcgccg
     2641 cgagggttcg tggcagcagt ggctttgtag gtaccatacc agtcggccgt ccactcccag
     2701 acgtttcccg ccatgtccag cgcgccaaac gggctcgctc ccgccggaaa gcgccccact
     2761 ggcgccgtgg actcccaggc gtcgtttgtg tcgtacatcg ggcgccagtc tgcgttcagc
     2821 tcgcgcttcg ccatcgccac gcactcgggg ccacaggcgt tcagccgggt cgcgctcggc
     2881 gtctcgttgc cccacggata cacacgtccg tccgtgccgc gcgctgcata ctcccactcg
     2941 gcctcgctcg gtagcctctt gtcggcccat gtgcagtatg ccgctgcctg gttccagtcc
     3001 acgcagttaa tcggatggtc cggtcgatcc tcgcggttgc agaaccggct gtagcgctgg
     3061 acgtcctgcg cagaaccccc gctccactgc accgtgagcg gcgcggcagt acagcccttg
     3121 gcctggacac acgccgcgta cgccttcacc gtcacctcgg tccggtcgat gcagtacgcc
     3181 gacagcgtca cgtcgtgccc cgggtgctcg tcgtcgtcgc ccgtcccggc gggactgccc
     3241 atccggaacg tccccgcgag caccgcgacc atgtccggcg ggcagcgcga cccgacggca
     3301 ttcgccgcgg cgtccactgt tccggcagtc cctgcatcgg tgggcggagt caacccgccg
     3361 gcctggcgcg cggtgccgcc cgtgggccgc tggagtaccg ccgcaatgcg gtacgccgcc
     3421 ggcgcgacca ccagggtggc tccgatcagc agggcccagc cgcgcaccag gcgcgcacgg
     3481 tcgggcagac cgagcgcgga gcgcgcgcgg cgcgcctgat ccgcgagc
//
LOCUS       DASVOR010002316         3921 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_229887,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002316 DASVOR010000000
VERSION     DASVOR010002316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229887"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VF516_42715"
     CDS             <1..601
                     /locus_tag="VF516_42715"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8114540.1"
                     /translation="YEVIAHPGTSITAHLSSGHETFDARFTTGPGAGPGPGIRGSWQG
                     DPHDFTRPMTSEISVDAASVDTGIDLRSTHAREEYLLANKFPKITVALDRVIAAAQAG
                     PGAIKFRAHGTLSLIGKTHSIEVTGTMKKPDAAALDRLHLTGSSAGSPAGSPAGDVLL
                     IQAEFPVVIKETALAKDAGDFDGEQIPIQVSLVMRHTSG"
     gene            642..1208
                     /locus_tag="VF516_42720"
     CDS             642..1208
                     /locus_tag="VF516_42720"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HEX8114541.1"
                     /translation="MKLLVLSTALALSVTAGPAFAAGPEPDSIVVLAKHTEAKPDDPV
                     HVQFTSFKVTKASFDPKKIEGGKATIEIDATSLKTGADKRDAHLNTPDFLDTKKFPKI
                     TIDVDKVKKKADKTFTAEATVKFRDVTKKYPVTFDVLDQTADSIRIKSEVSFSRLDFK
                     VGKESTDPKVAPAQNGLVMQMTLTLKKS"
     gene            1852..2898
                     /locus_tag="VF516_42725"
     CDS             1852..2898
                     /locus_tag="VF516_42725"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8114542.1"
                     /translation="MKTIGVDLWADQIRGSGNNNVTCLQPSATSRPRRGFRELFISSY
                     PGFAERCREVAEPGIALVAVDEVTGRPMGLVRLLARVQRHVAAIIGRHDECDLYLHAH
                     ARLALRHLCVVLDPVHDWRPGANVRYRVLDLRTTDGFTDENNRSLRGLRCEGPGLLRC
                     GGYALFIMPLGDPTDWPADPNDAWQFLPERVYFDELEKNPLGSVRRAPRVPQVPTLAS
                     AMTHRSLIIRTHGPRDTTDGLEDSGDIAGTLELISPRSRGTIRIGHDELRDGVLIGRY
                     ARCNNAQLADDPSLSRVHALLIQVDDAILVIDTASLNGTREADQPKARLIQLTGDTEL
                     VLGTSTRARWHWVS"
     gene            2910..>3921
                     /locus_tag="VF516_42730"
     CDS             2910..>3921
                     /locus_tag="VF516_42730"
                     /inference="COORDINATES: protein motif:HMM:NF024779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4105 domain-containing protein"
                     /protein_id="HEX8114543.1"
                     /translation="MTRRLAALAAALLCAALSGRASAAPGPRPGHQIDVPPEVMKLYE
                     DKPVIELVTMGVGSLIWERHGHIALCVQYDDRRHDCYNYGIGDFGHPIGMAWGFLRGT
                     GSFWVGKSGIGEMLSIYQYADRTIWVQPLPLTREEKQRVIDKLEHDIQEPYKYYAYDH
                     FWDNCTTRVRDVLNDATGGKLAAMHEPTDGKTFRDLAREGFFGMQIPLLITDIAMGRV
                     TDRAPTYYERMFLPQYLREAVTRLWGIQPIPVYERRGPPPLTDAPSGRVWFALVIVLL
                     TSPAWITRLVGRGQRAGLAVAVLPYVLLGTVLTALAILSPLTYVRWNEACLVLLPLDL
                     LVL"
BASE COUNT          636 a         1442 c         1287 g          556 t
ORIGIN      
        1 ctacgaggtc atcgcgcacc ccggcacgtc gatcaccgcg cacctgtcgt cgggccacga
       61 gacgttcgac gcgcggttta ccaccggccc cggggccggc cccggccccg gcatccgcgg
      121 cagctggcag ggcgacccgc acgacttcac ccggccgatg acctcggaga tcagcgtcga
      181 cgcggcctcg gtcgacaccg ggatcgacct gcggtcgacc cacgcgcgcg aggagtacct
      241 gctcgcgaac aagttcccga agatcacggt cgcgctcgac cgcgtgatcg cggcggccca
      301 ggccgggccc ggggcgatca agttccgcgc ccacggcacg ctgagcctga tcggcaagac
      361 tcactcgatc gaggtcaccg gcacgatgaa gaagccggac gcggccgcgc tcgaccggct
      421 ccacctcaca gggtcatcgg ccgggtcacc ggccgggtca ccggcgggcg acgtgctgct
      481 gatccaggcc gagttccccg tggtgatcaa ggagaccgcc ctcgccaagg acgccggcga
      541 cttcgacggc gaacagattc ccatccaggt gtcgctggtg atgcgccaca ccagcggctg
      601 acgatccgac tgtccggcgc atggagctga ccatgaaact gttgaagcta ctcgttctgt
      661 cgaccgcgct cgcgctctcc gtcacggccg gcccggcgtt cgccgccggt cccgagcctg
      721 attcgatcgt cgtcctcgcc aagcacaccg aggccaagcc cgacgatccc gtccacgtcc
      781 agttcacctc gttcaaggtc accaaggcga gcttcgatcc gaagaagatc gagggcggca
      841 aggcgacgat cgagatcgac gccaccagcc tcaagaccgg cgcggacaag cgcgacgcgc
      901 acctcaacac ccccgacttc ctcgacacca agaagttccc caagatcacc atcgacgtcg
      961 acaaggtgaa gaagaaggcg gacaagacgt tcaccgccga ggccacggtg aagttccgcg
     1021 acgtcaccaa gaagtacccg gtgacgttcg acgtcctcga tcagacggcc gacagcatcc
     1081 gcatcaagag cgaggtgtcg ttctcgcggc tcgacttcaa ggtcggcaag gagtcgacgg
     1141 accccaaggt cgctccggcc cagaacgggc tcgtcatgca gatgacgctg acgctcaaga
     1201 agtcctgagc gcccacgagc gccccggcgc ccggccaggc cggccaggcc gttagcggca
     1261 aacctggccg gggccgtcgg agaccgtcac gcaagttctg cccttggggc agaccgaatt
     1321 gtcctggcaa cggatctcac acgtcttgaa ctggggcccg cgcggaccgg cgaacccgta
     1381 gtagctgacg cacgtcagcc ccggcgcgca gcgatcgccc ggtccgcacg gctcctcgat
     1441 gccgggcgcc gaggacccag cgggcgcggc tggcaaagag gtcccgcccg gagcgggcgt
     1501 cgacggatcg atcgtcggcg ctggagcggg cgatgtcccc gggggcctcg gcgatgtcgg
     1561 cgcaggtagg ccgtttccgg gagacggtgc ggtgttttgc tgcggcccgg gcgccggcgc
     1621 ctcggaccct gcggccgaag ccaccggtcc cgatgtctcg cctgtcccgg atccgggcga
     1681 caccgacgga gaagagctgt gacaagcgat caggagcgcc aggatggcgc agtgcgcata
     1741 catgcccgtg tttcccactc cacgcgatct cacgcaactc cctaccccaa cgaatgccgc
     1801 acgcgggctc agtctctgct accgtcggct tatgcggggg cctggacgtc gatgaagacc
     1861 atcggggtcg acctgtgggc tgaccagatc cgcggcagcg gcaacaacaa cgtcacctgc
     1921 ctccagccgt cggcgacgtc ccgaccgcga cgcgggttcc gagagctctt catctcgtcg
     1981 taccccggct tcgccgagcg ctgccgcgag gtcgccgagc ccggcatcgc gctggtcgcc
     2041 gtcgacgagg tcaccggccg gccgatgggg ctggtccggc tgctcgcgcg cgtgcagcgc
     2101 cacgtcgccg cgatcatcgg gcgccacgac gagtgcgatc tgtacctcca tgcccacgcc
     2161 cgcctcgcgc tgcgccacct gtgcgtcgtg ctcgatccgg tgcacgactg gcggcccggc
     2221 gccaacgtgc gctaccgcgt gctcgacctg cgcaccaccg acgggttcac cgacgagaac
     2281 aatcgctcgc tgcgcggcct gcgctgcgag ggcccgggcc ttctgcgctg cggcggctac
     2341 gcgctgttca tcatgccgct cggcgatccg acggactggc cggccgaccc gaacgacgcc
     2401 tggcagttcc tgcccgagcg ggtgtacttc gacgagctcg agaagaaccc gctcggctcg
     2461 gtgcgccgcg ccccgcgggt cccgcaggtg ccaacgctgg cgagcgcgat gacccaccgc
     2521 agcctgatca tccgcaccca cggcccgcgc gacaccaccg acggcctgga agacagcggc
     2581 gacatcgccg gcacgctcga gctgatcagc ccgcgcagcc gcggcacgat ccggatcggc
     2641 cacgacgagc tccgtgacgg cgtgctgatc ggccgctacg cgcgctgcaa caacgcccag
     2701 ctcgccgacg acccgtcgct gtcgcgggtc cacgcgctgc tgatccaggt cgacgacgcg
     2761 atcctggtga tcgacaccgc cagcctcaac ggcacccgcg aggccgacca gcccaaggcg
     2821 cggctgatcc agctgaccgg cgacaccgag ctggtgctcg gcacctcgac gcgtgcgcgc
     2881 tggcactggg tgtcgtaagc tccccgacga tgactcgacg cctcgccgcg ctggcggccg
     2941 cgctcctgtg cgctgcgctg tccgggcgcg catccgccgc cccgggcccc cggccgggcc
     3001 accagatcga cgtcccgccc gaggtcatga agctgtacga ggacaagccg gtgatcgagc
     3061 tggtgacgat gggcgtcggc tcgttgatct gggagcgcca tggccacatc gcgctgtgcg
     3121 tgcagtacga cgaccggcgc cacgactgtt acaactacgg catcggcgac ttcggccatc
     3181 cgatcgggat ggcctggggc ttcctccgcg gcaccggcag cttctgggtc ggcaagtccg
     3241 gcatcggcga gatgctgtcg atctaccagt acgcggatcg cacgatctgg gtccagcccc
     3301 tgccgctgac ccgcgaggag aagcagaggg tcatcgacaa gctcgagcac gacatccagg
     3361 agccgtacaa gtactacgcc tacgatcact tctgggacaa ctgcacgacc cgggtccgcg
     3421 acgtcctcaa cgacgccacc ggcggcaagc tggcggcgat gcacgaaccg accgacggca
     3481 agacgttccg cgatctcgcc cgcgaggggt tcttcggcat gcagatcccg ctgctgatca
     3541 ccgacatcgc gatgggccgc gtcaccgacc gcgcgccgac gtactacgag cggatgttcc
     3601 tgccgcagta cctgcgcgag gcggtgacca ggctgtgggg catccagccg atcccggtct
     3661 atgagcgccg gggccccccg ccgctcaccg acgcaccgag cggccgggtg tggttcgcgc
     3721 tcgtcatcgt gctgttgacc tcgccggcgt ggatcacgcg cctggtcggc cgcggccagc
     3781 gcgccgggct cgccgtcgcc gtgctcccgt acgtcctgct cggcaccgtg ctgaccgcgc
     3841 tggcgatcct cagcccgctg acctacgtgc ggtggaacga ggcctgcctg gtgctgctgc
     3901 cgctcgatct gctcgtgctg g
//
LOCUS       DASVOR010002317         1856 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_83856,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002317 DASVOR010000000
VERSION     DASVOR010002317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83856"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(81..209)
                     /locus_tag="VF516_42735"
     CDS             complement(81..209)
                     /locus_tag="VF516_42735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114544.1"
                     /translation="MVARGALVLERHVGAADRATPLVELEVRARRSSTSRSQRAWS"
     gene            1128..1826
                     /locus_tag="VF516_42740"
     CDS             1128..1826
                     /locus_tag="VF516_42740"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8114545.1"
                     /translation="MVLASLAGCADNLVTHTAPETPTTGTDRATVIGHVLDATGAPVA
                     GATVTVRASGEHATADSTGAFTLDVPANTTLTLAANAPSMAPTLLQQFLVSTGTTAQV
                     EIPLITSDHFQTLVAMGMNPSGGVVAVALKSLSGAGSVAGATVQLTPSLGRVMYAPSL
                     GMADPDPTMAAVVQGDDSYAWALGVQPHVSIMQLALRGAAQVEPPYAIDDVIWPGTFT
                     VDAGALTLVTLFTK"
BASE COUNT          297 a          686 c          602 g          271 t
ORIGIN      
        1 tgatacctac cgcgcgctgg acgcagtagc gagtccgcgc gccgccccgg gttgcggatg
       61 cgaaccatgc agccgaggtg tcacgaccac gcgcgctggc ttcgtgacgt cgatgaccgg
      121 cgcgcccgca cctcgagctc cacgagcggg gtcgcgcggt cggcggcgcc gacgtggcgc
      181 tcgaggacca gcgcgccccg cgcgaccacc tgcgcatcga cgccgcgcac cgcgatcgcg
      241 gcggtaacgg gaacccgtgc acgcggtcgc cgtggccagc gagcgcatcg gcaggcacac
      301 gcgggcacgc gaggccggac cacgacggca gcggcgagct cgtccgccgg gaccagagcc
      361 agagccagag ccagagcctc gccctgccgg cgagggcgcg ggagcaagcc tctgctcgag
      421 gcaccgatac gatagcggaa caaactgttc gctcaggaca gggcgaacgt gcctgacccc
      481 aggcagcgat cattgcgcgt cttcatcggt gtcgcgcgat cagacggcga actgaacgaa
      541 cgtctcgccc gcgtccgccg tggactcttg cggcgaatcg aacgcgcggc gacgtgaaat
      601 cacctcgtcc ggatccggaa ggagacccgc gggctgcatg aaaacgcgcc aggcggtgca
      661 ccctggacat tcggagtacc ccctggacat tcgcagtgaa cgcgtgagga gcgcgttccg
      721 cggccgggcg cccggccgcc gatcagcgag ccctctgacc gaagtgtgag gccgaggcaa
      781 gcgcagttgc gtgaggttga gggcagttga gcgtgaggtg gcctgacggt agtgaccgcg
      841 cagcgcatgt agggttcgcg tcgagtgccg cggacattgc ctcctggacc tacgcggcga
      901 cgtccaggtt cggtcgccgc tcgccttgcc gacgatcgcg cggccgacct gccgtctgct
      961 catggaagtc ttcgagaaga aagctgatct gcccgctcct cgccgcgcgc agggcggcgc
     1021 tctgggtttc ggcattgtcc gtgcaaaggg gccttgtcat ccaaaacgac acgtaatctg
     1081 aacaacggat ttttgtcatg agtaactgtc atcgagtctg ttttctggtg gtcctggcct
     1141 ctctcgccgg ttgcgcggac aacctggtga cccacactgc ccccgaaacg cctaccaccg
     1201 gcaccgaccg tgccacggtc atcggccatg tcctggatgc caccggcgcc cccgtcgccg
     1261 gcgcgacggt gaccgtccgc gcctccgggg agcacgccac cgcggacagc accggcgcgt
     1321 tcaccctcga cgtcccggcc aacaccacgc tgaccctcgc cgcgaacgcg cccagcatgg
     1381 cgcccaccct cctccagcag ttcctggtct cgaccggcac caccgcccag gtggagatcc
     1441 cgctgatcac cagcgaccac ttccagaccc tggtcgcgat gggcatgaac ccctcgggcg
     1501 gcgtggtcgc cgtcgccctc aagtcgctgt cgggcgccgg cagcgtcgcc ggtgccaccg
     1561 tccagctcac cccgagcctg ggccgcgtga tgtacgcgcc cagcctgggc atggccgacc
     1621 ccgaccccac gatggcggcc gtcgtgcagg gcgacgactc ctacgcctgg gcgctcggtg
     1681 tccagcccca cgtctccatc atgcagctcg cccttcgcgg cgcggcccag gtcgagcccc
     1741 cctacgccat cgacgacgtc atctggcccg gcaccttcac cgtcgacgcc ggcgcgctca
     1801 ccctcgtcac gctgttcacc aagtgaggtc tccgatgaag cccgtgtaca ccctcg
//
LOCUS       DASVOR010002318         4019 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_287121,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002318 DASVOR010000000
VERSION     DASVOR010002318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287121"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(348..1328)
                     /locus_tag="VF516_42745"
     CDS             complement(348..1328)
                     /locus_tag="VF516_42745"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triacylglycerol lipase"
                     /protein_id="HEX8114546.1"
                     /translation="MSKVLVSSLGLALAVASVSAVSSCASASGASDEASAAVPEAAST
                     FTQTRYPIVLAHGLTGFRQLFGVVDYFNGIPSDLRSGGAQVFVTQVSAVASATERGEQ
                     LLQQIETIVATTGAGKVNLIGHSEGGLDARYVMGVRPDLIASLTTVATPHLGADLADF
                     LAENVAGSSFNQTVLSLFGNSLGTIIDLLTGMPSQQDAIGALNSLDSAGAAVFNRQFP
                     AGLAATRCGATPSSVNGIQLFSWAGSSQLTNLLDVSDPALGLTSLVYSEANDGLVGRC
                     SAHFGKVIRDNFGMNHLDEVNQLFGLTSIFETSPVSVFRSHANRLKNAGL"
     gene            complement(1554..2117)
                     /locus_tag="VF516_42750"
     CDS             complement(1554..2117)
                     /locus_tag="VF516_42750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114547.1"
                     /translation="MRFGADAWGRLAAVPAFVLVLPWQCSLAPRGDPGLLGASVAPTP
                     EPARPPPNAPQDPAHDLAHDPVPPAPSAATPKQEQPEPRVPAVPPPVPPVRAVSLPDE
                     VVVQTIGAGQQAFLRCWARAQRSDAPPTADKVRLHLEIDEQGRVAAASSDSDSPALDR
                     CLQVVARRLPFPPPGRPAVVDLPLMFH"
     gene            2361..3158
                     /locus_tag="VF516_42755"
     CDS             2361..3158
                     /locus_tag="VF516_42755"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8114548.1"
                     /translation="MAPHVLVADDDAWILRMVATVLEKRGYSVETAVDGEDALHRALA
                     RTPDLLITDVMMPKMDGWSLVRNLRAHSDLALLPVIFLTALSSEDDRIRGFRLGADDY
                     VTKPFRFEELDLRVAKTLRRTQASVQEAREHLGGAGLRGDLSQVGLSSLLILIEMERK
                     TGLLQLRAPNGPSAQVLVRDGKVVHARLDNAEDPVDAECIYYLLTWGAGEFEFVMCLV
                     EGSDRVNISTTHLLMEGARLMDESREPSAHQPIARIPPLGDPPDDAL"
     gene            complement(3172..>4019)
                     /locus_tag="VF516_42760"
     CDS             complement(3172..>4019)
                     /locus_tag="VF516_42760"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8114549.1"
                     /translation="FEDAPTSPSPGASGRDGDACERARRALHAASGGTLLVKDVSDLS
                     ATSQRTLRRAIRGRGERDSGELFDVQVLATADLDLDRAVDASIVSPEFYQVFADRRIE
                     VPPLRDRSDDLPELFERWIKHYSAEIGRTKPTVSSRAVARLTAYPWPGNVAELKAIAR
                     RLVVRVAGSRIEAGDVDEILPVVAERVPLEDLAFEDMVKAKLAGLLARIDGYPVHDLY
                     DKVLARVERPLFDLVLAHTGGNQLKAAEILGLNRNTLRKKLAGLGIEQAKKRAGREDR
                     ALDGK"
BASE COUNT          587 a         1392 c         1457 g          583 t
ORIGIN      
        1 agaagatctt ccagatcacc tacgccttgc gtccggggga tccgacgtac tttcgcgacc
       61 acgtcgagtt cctcgccgga gctcaggccg cggacgacaa gcccgtacgc agtacccctg
      121 ccgggcggtc gatctcaagc gctcctgcgg acgcagcatc ggcggcagcg gcgccgacgt
      181 cgaccaccag cgcagcggcg tgggcagtgc tgtgccgggg cctggccagc cggcgccgga
      241 gcggccgcgt catccccagc gtcgcctcgg gccagctata ccctccggct gaggcgtacg
      301 tcgctcccgc tcggcgagta gcgcggccgt cgggccgtcg cccgcgatca gaggccggcg
      361 ttcttgagcc ggttggcgtg cgagcggaac accgagaccg ggctggtctc gaatatcgag
      421 gtcaggccga acagctggtt gacctcgtcg aggtggttca tcccgaagtt gtcgcgaatg
      481 accttgccga agtgcgcgct gcaccggccg accaggccgt cgttggcctc ggagtagacc
      541 agcgaggtca ggccgagcgc cgggtcggag acgtcgagca ggttggtgag ctggctggag
      601 ccggcccacg agaacagctg gatgccgttg accgagctcg gcgtcgcacc gcaccgggtc
      661 gcggcgaggc cggccgggaa ctggcggttg aacacggccg cgcccgccga gtccagcgaa
      721 ttcagcgcgc cgatcgcatc ctgctgcgac ggcatgccgg tcagcaggtc gatgatcgtg
      781 cccaggctgt tgccgaacag ggacagcacg gtctggttga agctgctgcc ggcgacgttc
      841 tcggccagga agtcggcgag gtcggcgccg aggtgcggcg tcgcgaccgt ggtgagcgag
      901 gcgatcaggt cgggccgcac gcccatcacg tagcgcgcgt cgaggccgcc ctcggagtgg
      961 ccgatcaggt tgaccttgcc ggcgccggtg gtggcgacga tggtctcgat ctgctgcagc
     1021 agctgctcgc cgcgctcggt cgcggacgcg acggcgctga cctgggtcac gaacacctgc
     1081 gcgccgccgg accgcagatc gctcgggatg ccattgaagt agtcgaccac gccgaacagc
     1141 tggcgaaatc cggtcaggcc gtgcgccagg acgatcggat agcgcgtctg ggtgaaggtc
     1201 gacgcggcct ccggcaccgc ggcgctggcc tcgtcggaag caccgctcgc cgaggcgcag
     1261 gatgaaactg cggacacgct ggcgacggcc agcgcgaggc caagactcga cacaagcacc
     1321 ttcgacatgg ctgcctgtat atgttgtccc gtgcggcgct gtcgccggta aaatcgaatc
     1381 tttgtattgc tccgagactg acaatgatta gcgtgtagtg cgccctacca agatcacatg
     1441 ttgacgcgta tggtcagacc acccggggcg tcgaatcggc cggaccatcc gcccggtcag
     1501 cggcggacag ccgctgtcac cgggcggtga tcgcgacggg tcgccggcgg tcctcagtgg
     1561 aacatcagcg gcagatcgac gacggcgggg cgacccggcg gagggaacgg cagccgccgc
     1621 gcgacgacct gcaggcagcg gtcgagcgcc ggcgagtcgc tgtcgctgct cgcggcggcg
     1681 acccggccct gctcgtcgat ctcgaggtgc agccggacct tgtcggcggt cggtggcgcg
     1741 tcgctgcgct gcgcgcgcgc ccagcagcgc aggaacgcct gctgccccgc gccgatcgtc
     1801 tggacgacga cctcgtcggg cagcgagacc gctcgcacgg gcggtaccgg cgggggcacc
     1861 gccgggaccc ggggctcggg ctgctcctgc ttgggggtcg cggccgacgg tgccggcggc
     1921 acgggatcat gcgcgaggtc atgcgccgga tcctgcggag cgttcggcgg cgggcgagcc
     1981 ggctcgggcg tcggcgcgac ggacgcaccg agtagaccgg ggtcgccccg cggtgcaagg
     2041 ctgcactgcc acgggagcac gaggacgaag gccggaaccg cggcgagccg cccccaggcg
     2101 tctgcgccga aacgcatgga tggcagtgtc ccacggatcg ctgcacggcg catggacgtg
     2161 tcgagccggg gctgtcccac atcacacgaa ccggcgaacg cgtcgcccgt cacgggccgt
     2221 ccagccacaa tgcaacatcg ccgcgagtgt gcaatccccg gcgatgtcag acctgcggca
     2281 agcccctggg gacgcgccaa ccccgcgatc gccgtagggt tgcttcggcg aagcctggca
     2341 ggctacggtg cagatcagcg atggccccgc atgtcctggt cgccgacgat gacgcttgga
     2401 tcttgcgcat ggttgccacg gtgctcgaaa agcgcggcta cagcgtcgag acggcggtcg
     2461 acggggagga cgcgctgcac cgggcgctcg cccgcacccc ggatctgctg atcaccgacg
     2521 tgatgatgcc gaagatggac ggctggtcgc tggtgcggaa cctgcgggca cactccgacc
     2581 tcgcgctcct gccggtgatc ttcctcaccg cgctttcttc cgaggatgac cggatccgcg
     2641 gcttccggct cggcgcggac gactacgtca ccaagccgtt tcggttcgag gagctcgacc
     2701 tccgggtcgc caagaccctg cggcggaccc aggcgtcggt ccaggaggct cgcgagcacc
     2761 tcggcggcgc cgggctgcgc ggcgacctgt cgcaggtcgg gctgtcgtcg ctgctgatcc
     2821 tgatcgagat ggagcgcaag accgggctgc tccagctgcg cgcgcccaac ggcccgtcgg
     2881 cccaggtcct ggtgcgcgac ggcaaggtcg tccacgcccg gctcgacaac gccgaggacc
     2941 cggtcgacgc cgagtgcatc tattacctct tgacctgggg cgccggcgag ttcgagttcg
     3001 tgatgtgcct ggtcgagggc tccgatcggg tcaacatctc gaccacccac ctgctgatgg
     3061 agggcgcccg gctgatggac gagtcgcgcg agccgtccgc gcaccagccg atcgcgcgga
     3121 tcccgccgct cggcgatccg cccgacgacg cgctgtagcg ccgccaggag atcacttgcc
     3181 gtcgagcgcg cggtcctcgc gtccggcgcg cttcttggcc tgctcgatgc cgaggccggc
     3241 gagcttcttg cgcagcgtgt tgcggttgag cccgaggatc tcggcggcct tgagctggtt
     3301 gccgccggtg tgggcgagca cgaggtcgaa cagcgggcgc tcgacccgcg ccagcacctt
     3361 gtcgtagagg tcgtgcaccg gatagccgtc gatccgggcg agcagacccg cgagcttggc
     3421 cttgaccatg tcctcgaacg cgaggtcctc gagcgggacg cgctcggcga cgaccggcag
     3481 gatctcgtcg acgtcgcccg cctcgatccg cgatcccgcg acccgcacga ccagccgccg
     3541 cgcgatcgcc ttgagctcgg cgacgttgcc cggccacggg tacgcggtca gccgcgcgac
     3601 cgcccggctc gacaccgtcg gcttggtccg cccgatctcg gcggagtagt gcttgatcca
     3661 gcgctcgaac agctcgggca ggtcgtcgct gcggtcgcgc agcggcggga cctcgatccg
     3721 gcgatcggcg aacacctggt agaactcggg cgagacgatc gatgcgtcga ccgcgcgatc
     3781 gaggtcgagg tccgcggtgg cgagcacctg gacgtcgaac agctcgccgc tgtcgcgctc
     3841 gccgcggccg cggatcgcgc ggcgcagcgt gcgctgcgag gtcgccgaca gatcgctgac
     3901 gtccttgacc agcagcgtcc cgccgctcgc cgcgtgcagc gcgcgccgcg cccgctcgca
     3961 ggcgtcgccg tcgcggccgc tcgcgcccgg gctcgggctg gtcggcgcgt cctcgaacg
//
LOCUS       DASVOR010002319         2153 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_317654,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002319 DASVOR010000000
VERSION     DASVOR010002319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2153)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2153)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2153
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317654"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VF516_42765"
     CDS             <1..1083
                     /locus_tag="VF516_42765"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HEX8114550.1"
                     /translation="AVPQGAAARWPPFAAGVTIDAAAAVNLVWTDVADQSEVARQLPG
                     ELAAARASGLSAVVTTVAPQGRFWLDDAAFEKTKRDLAAWKAGIAEHPDALLLVERAA
                     DLEAARQGRRLGVVFTFQGTEPLGEDPDRIPMFRELGVRVIQLTHNRRNIVGDGCMEP
                     GNAGLSNYGHTVVERLNAARVVVDLAHGAQRTIREGIAASKAPMLISHTGCRALSDLP
                     RCTPDAELRMLADKGGVAGMIFWPYLRTDTQPMAIDLVRHIEHAINVAGEDHVGIGSD
                     TSLAPVERTAAYERDNRAMVADMVDQGIFGRGRPADLYMFLPDLNLANRYEVLGAMLA
                     ARGHSDARIAKILGGNVARVMREVWG"
     gene            1080..1322
                     /locus_tag="VF516_42770"
     CDS             1080..1322
                     /locus_tag="VF516_42770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114551.1"
                     /translation="MKVVSVEEAEIDLSRLIQEALEGEEIVIARGNDALVRLVPVESS
                     RPRRGVGWARGQVTMAPDFDAPLDDFMIDIDNDLSK"
     gene            1376..>2153
                     /gene="coaBC"
                     /locus_tag="VF516_42775"
     CDS             1376..>2153
                     /gene="coaBC"
                     /locus_tag="VF516_42775"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEX8114552.1"
                     /translation="MTDGPRSERPPRDPAHPLQGARIVVGVAGGIAAYKAAELVRLLD
                     KAGATVDVAMTAGAQKFDGPMTFQALTRRPVFTDLFSLTEEAEIGHIQLADRADLIVI
                     APATANALARLAAGQADDPVAAIALATRAPVLVAPSMNVHMWSHPLTRANLVRLVEVA
                     GYRVVGPGDGFLACRWTGPGRLAEPVDIVEAAAHVLSPQDLAGRRVVVTAGPTHEAID
                     PARFIGNRSSGKMGVALAAAAQRRGAEVALLLGPSAIAPPV"
BASE COUNT          299 a          749 c          813 g          292 t
ORIGIN      
        1 gcggtgcctc agggagcggc ggcgcgctgg ccgccgttcg ccgccggcgt gacgatcgac
       61 gcggcggccg ccgtgaatct ggtctggacc gacgtcgcgg atcagtccga ggtcgcccgg
      121 cagctccccg gcgagctggc cgcggcgcgc gcatcggggc tgtccgcggt ggtgaccacc
      181 gtggcgccgc aggggcggtt ctggctcgat gatgccgcgt tcgagaagac caagcgcgac
      241 ctcgcggcat ggaaggccgg gatcgccgag catcccgacg cgctgttgct ggtcgagcgc
      301 gccgccgatc tggaggccgc gcggcagggc cgccggctcg gcgtggtgtt cacgttccag
      361 ggcaccgagc cgctcggcga ggaccccgac cggatcccga tgttccgcga gctcggcgtg
      421 cgggtcatcc agctcaccca caaccgccgc aacatcgtcg gggacggctg catggagccc
      481 ggcaacgccg gcctgagcaa ctacggtcac acggtcgtcg agcggctcaa cgcggcccgg
      541 gtcgtcgtcg atctggcgca cggcgcgcag cgcacgatcc gcgagggcat cgccgcgtcg
      601 aaggcgccga tgctgatcag ccacaccggc tgccgggcgc tgtccgacct gccgcgctgc
      661 accccggacg ccgagctccg catgctcgcc gacaagggcg gcgtcgccgg catgatcttc
      721 tggccgtacc tgcgcaccga cacccagccg atggcgatcg atctcgtccg ccacatcgag
      781 cacgcgatca acgtcgcggg cgaggaccac gtcggcatcg gcagcgatac ctcgctggct
      841 cccgtcgagc gcaccgccgc gtacgagcgc gacaaccgcg cgatggtcgc cgacatggtc
      901 gaccagggca tcttcggccg cggccgcccg gccgacctct acatgttcct ccccgatctc
      961 aacctcgcga accgctacga ggtgctcggc gcgatgctgg cggcgcgcgg ccactccgac
     1021 gcgcggatcg ccaagatcct cggcggcaac gtcgcccgcg tcatgcgcga ggtgtgggga
     1081 tgaaggtcgt cagcgttgag gaagccgaga tcgatctgtc gcgcctgatc caggaagcgc
     1141 tggaagggga ggagatcgtg atcgcacgcg gcaacgatgc gctggtgcgg ctcgtcccgg
     1201 tcgaatcatc tcggccccga cgaggtgttg gttgggccag gggccaggtg acgatggcgc
     1261 ccgacttcga cgcaccgctc gacgacttca tgatcgacat cgataacgac ctatcgaagt
     1321 gaggtgtggt gatgcccgtg gggcgcccgg cgacggggcg gggtaggctt gcggcgtgac
     1381 cgacggacct cgcagcgaac gaccgcctcg cgacccggcg catccgctgc agggcgcgcg
     1441 gatcgtggtc ggcgtcgccg gcgggatcgc ggcgtacaag gcggccgagc tggtgcggct
     1501 gctcgacaag gcgggcgcga cggtcgatgt cgcgatgacc gcgggcgccc agaagttcga
     1561 cgggccgatg acgttccagg cgctgacccg gcgcccggtg ttcaccgacc tgttcagcct
     1621 gaccgaggag gcggagatcg ggcacatcca gctcgcggat cgggccgatc tgatcgtgat
     1681 cgcgccggcg acggccaacg cgctggcgcg gctggcggcg gggcaggccg acgatccggt
     1741 cgcggcgatc gcgctggcga cccgcgcgcc ggtgctggtc gcgccgtcga tgaacgtcca
     1801 catgtggagc cacccgctga cccgggcgaa cctggtgcgc ctggtcgagg tcgccgggta
     1861 tcgcgtggtc ggcccgggcg acggcttcct ggcgtgccgg tggaccggcc cggggcggct
     1921 cgccgagccg gtggacatcg tcgaggcggc ggcgcacgtc ctgtcaccgc aggacctggc
     1981 gggccggcgc gtcgtggtca ccgccgggcc gacccacgag gcgatcgatc cggcgcggtt
     2041 catcggcaac cggagctcgg gcaagatggg cgtggcgctg gcggccgccg cgcagcggag
     2101 aggtgccgag gttgcgctgc tgctcggccc gagcgcgatc gcgccgccgg tcg
//
LOCUS       DASVOR010002320         2129 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_83861,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002320 DASVOR010000000
VERSION     DASVOR010002320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_83861"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VF516_42780"
     CDS             complement(<1..262)
                     /locus_tag="VF516_42780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114553.1"
                     /translation="MRVVLAAVLLGSVAAAAPAPAPPAQPATGKPAAPPLWLGVFDPK
                     LAAHPPWDNAKLTPVEHGGAVRVLAPDHGPAASGDVVVVHPLL"
     gene            complement(328..777)
                     /locus_tag="VF516_42785"
     CDS             complement(328..777)
                     /locus_tag="VF516_42785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8114554.1"
                     /translation="MEINGIAHAILTVRDMAVSSPFYRALLAHLGLSEIVNTEIYLYY
                     VGGRTAVGIRPAAPPLAQHRFDQERCGLHHLCFRARSREDVDSVFAAVTRLGARIVHP
                     PEEGPWVPGYYSVLFEDPDGIRLEVNHVPGKGVFDQGVQRGDVATGV"
     gene            complement(817..1233)
                     /locus_tag="VF516_42790"
     CDS             complement(817..1233)
                     /locus_tag="VF516_42790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114555.1"
                     /translation="MIRWLRFLATATVVLSMIGLGVIAGLFLVANGGWIPVTTPPWLT
                     GLLGDRPHEIWLPALIAGWFAGAVLLGALVLTTLFAIWRRRQYESLVHRLERELARLR
                     NLPFEEPAPLEDLPEMPSTEAARVMHALDDEDRDAP"
     gene            complement(1352..>2129)
                     /locus_tag="VF516_42795"
     CDS             complement(1352..>2129)
                     /locus_tag="VF516_42795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEX8114556.1"
                     /translation="SEMSWTRRVKHPSKMVAVGDMVEAVVLDVDVKQNRISLGMKQLE
                     PNPYEQLTEKYPPGTVVKGHVRNIADFGIFVEIEEGIDGLVHISDMSWTQRVKHPSEL
                     FQKGDEVEAVLLNIDTGDGDKPKISLGIKQLVPDPWDRIPYDYPQGKIVDGKVLKVLD
                     FGAFVELEKGIEGLVHVSEISEDHVEDPRTVLQPGAVVKVQVLHADGAERKIGLSIKG
                     AARAKDSAEAQGFNSSNAGGATLGDKFRGKLDKLKPREQQ"
BASE COUNT          302 a          775 c          744 g          308 t
ORIGIN      
        1 cgagcagcgg gtgaaccacc accacgtcgc cgctcgccgc gggcccgtgg tccggcgcca
       61 gcacgcgcac ggcgccgcca tgctccaccg gcgtcagctt cgcgttgtcc cacggcgggt
      121 gcgccgccag cttcggatcg aacacgccga gccacagcgg cggcgccgcc ggcttgccgg
      181 tcgccggctg ggcaggtggc gccggggccg gtgcagcggc cgcgacgctc ccgagcaaca
      241 cagctgccag aacgactctc acccctacag catgcgccac ggcgcgcgca ccgcaactcg
      301 ctgcggccgc ggcgcacgag cggtgcgtca aacgccggtc gcgacgtcgc cgcgctggac
      361 gccctggtcg aacacgccct tgccgggcac gtggttgacc tcgagccgga tcccgtcggg
      421 atcctcgaac agcaccgagt agtagcccgg cacccagggg ccctcctccg gcgggtgcac
      481 gatcctggcg ccgagccggg tgaccgccgc gaacaccgag tcgacgtcct cgcggctgcg
      541 cgcgcggaag cacaggtggt gcaggccgca gcgctcctga tcgaagcggt gctgtgcgag
      601 cggcggcgcc gccgggcgga tgcccaccgc ggtgcggcct ccgacgtagt agaggtagat
      661 ctcggtgttg acgatctcgg acaggccgag gtgcgccagc agcgcccggt agaacgggct
      721 ggagaccgcc atgtcgcgga cggtcaggat ggcgtgggcg attccgttga tctccatggc
      781 tgcctctcgg ttggctcggg cgccgcgccg ccgcggctac ggcgcgtcgc gatcctcgtc
      841 gtcgagcgcg tgcatgacgc gcgcggcctc ggtgctcggc atctccggca ggtcctcgag
      901 cggcgcgggc tcctcgaacg gcaggttgcg cagccgggcg agctcgcgct ccagccggtg
      961 gaccagcgac tcgtactgcc gacgccgcca gatcgcgaac agcgtggtca gcaccagcgc
     1021 gccgagcagc acggcgccgg cgaaccagcc ggcgatcagc gcgggcagcc agatctcgtg
     1081 cggccgatcg ccgagcagcc cggtgagcca cggcggcgtc gtcaccggga tccagccgcc
     1141 gttggccacc aggaacaggc cggcgatcac gccgaggccg atcatcgaca gcaccacggt
     1201 cgcggtggcg aggaagcgca gccagcggat cacgcggcca ctgtactacg ccgcacgcac
     1261 catgccccgg ctagactcga ggcggccgaa accgcgagag ccgcccgcgg cacgggcggc
     1321 tcgccggagc tcgcggcgcg aagcgcgcgg gctactgctg ctcgcgcggc ttgagcttgt
     1381 cgagcttgcc gcggaacttg tcgcccagcg tggcgccgcc ggcgttcgac gagttgaagc
     1441 cctgggcctc ggcggagtcc ttggcccgcg ccgcgccctt gatcgacagg ccgatcttgc
     1501 gctcggcgcc atcggcgtgg agcacctgga ccttcaccac cgccccgggc tgcagcacgg
     1561 tgcgcggatc ctcgacgtgg tcctcgctga tctcggacac gtggaccagg ccctcgatgc
     1621 ccttctccag ctcgacgaac gcgccgaagt cgagcacctt gagcaccttg ccgtcgacga
     1681 tcttgccctg cgggtagtcg tacgggatcc ggtcccacgg atcggggacc agctgcttga
     1741 tgcccagcga gatcttcggc ttgtcgccgt cgccggtgtc gatgttgagc agcaccgcct
     1801 cgacctcgtc gcccttctgg aacagctcgc tcgggtgctt gacccgctgg gtccacgaca
     1861 tgtcgctgat gtggaccagg ccgtcgatgc cctcctcgat ctcgacgaag atgccgaagt
     1921 ccgcgatgtt gcggacgtgg cccttgacca cggtgcccgg cgggtacttc tcggtcagct
     1981 gctcgtaggg gttgggctcg agctgcttca tgccgagcga gatccggttc tgcttgacgt
     2041 cgacgtcgag cacgaccgcc tcgaccatgt cgccgaccgc gaccatcttc gacgggtgct
     2101 tgacccgccg ggtccacgac atctcggag
//
LOCUS       DASVOR010002321         1605 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_346122,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002321 DASVOR010000000
VERSION     DASVOR010002321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346122"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VF516_42800"
     CDS             complement(<1..276)
                     /locus_tag="VF516_42800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114557.1"
                     /translation="MKLAALLLLVAACDAQVDGSYAGDAYVRLRGITVGFSADAAIDG
                     AAVRWTSQTGAALDAGPPTPLPLESAPPAVILPVVSRPPEAAMFGFAG"
     gene            complement(273..1226)
                     /locus_tag="VF516_42805"
     CDS             complement(273..1226)
                     /locus_tag="VF516_42805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114558.1"
                     /translation="MRTILVASIFASPVASLVASLAALGACASSPEPAPPPMTTARAA
                     DLADGGVPSTASACLARYSYRWLDLQIDCPTEHVGGDPDHWVTSCAIPAGFGSLDLHG
                     RDVPPLRYEATSEAGFLLHEVIDMPPGLRLGYVPAHDVSYVYDAQHRLAERIALDVNG
                     VEINHIVVGPRDAAGNPQSTAFTTLPLVVYGVTFPATAHSTHVYRYDANGRLVTDQGT
                     FSDGVAFWDETVHYDDAALRRDRLVIFDVSNEIHVTGPGYNTTHELTDAGGRLLQLDH
                     TTDGDTPYVVDYHYDDRSRLTRTIATRDGFTETVDYIYECP"
     gene            1399..>1605
                     /locus_tag="VF516_42810"
     CDS             1399..>1605
                     /locus_tag="VF516_42810"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEX8114559.1"
                     /translation="MESAFAIIAEPNRRAILSLLASTERSVGEIERQLHMSQPSVSKH
                     LRILRDAGFVESRVDAQRRVYRIRP"
BASE COUNT          248 a          536 c          592 g          229 t
ORIGIN      
        1 gccggcgaag ccgaacatcg ccgcctcggg cgggcgcgac acgaccggca ggatcacggc
       61 cggcggcgcg gactcgagcg gcagcggcgt cggcggcccc gcatcgagcg cggcgcctgt
      121 ctgcgacgtc cagcgcaccg cggcgccgtc gatcgcggcg tccgccgaga acccgaccgt
      181 gatgccgcgc aggcgcacgt aggcgtcgcc ggcgtagctc ccgtcgacct gcgcgtcgca
      241 cgccgccacc agcagcagca gggccgcgag cttcatgggc actcgtagat gtagtcgacc
      301 gtctccgtga agccgtcccg cgtcgcgatc gtcctggtca ggcgcgagcg gtcgtcgtag
      361 tggtagtcga cgacgtacgg cgtgtctccg tccgtcgtgt ggtccagctg gagcaagcgg
      421 cctccggcgt cggtgagctc gtgcgtcgtg ttgtagcccg ggcccgtgac gtggatctcg
      481 ttgctgacat cgaagatcac gaggcggtcg cggcgtagcg cagcgtcatc gtagtggacg
      541 gtctcgtccc agaacgccac gccgtcggag aacgtgccct gatcggtgac caggcggccg
      601 ttcgcgtcgt agcggtacac gtgggtgctg tgggcggtcg ccgggaacgt cacgccgtac
      661 accacgagcg gcagggtcgt gaacgccgtc gactgcgggt tgccggcggc atcgcgcggt
      721 ccgacgacga tgtgattgat ctcgacgccg ttcacgtcga gcgcgatgcg ctcggccagg
      781 cggtgctggg cgtcgtacac gtacgacacg tcgtgcgccg gaacgtagcc gagcctcagg
      841 cccggcggca tgtcgatcac ctcgtgcagc agaaagccgg cctcgctggt ggcctcgtag
      901 cgcagcggcg gaacgtcccg gccatgcagg tccagcgagc cgaagcccgc cgggatcgcg
      961 cacgacgtca cccagtggtc gggatcgccg ccgacgtgct cggtcggaca gtcgatctgg
     1021 aggtcgagcc agcgatagct gtaccgggcg aggcacgccg atgcggtcga cggcacaccg
     1081 ccgtccgcga ggtcggccgc gcgcgccgtg gtcatcggcg gtggcgcggg ctcggggctc
     1141 gaggcgcatg cgccgagcgc ggcgagagaa gcgacgaggg aagcgaccgg ggaagcgaag
     1201 atggaagcga cgaggatcgt ccgcatggtg acgatcagga ccgccagccg gccggatcgt
     1261 gcaatcaggg taacgcgccg agcgccgcac cgatcctggc aagatcgcgg aggtcttccg
     1321 cagccccggg gcattgcgcg agcccaggcg gcggtgcatg gcttgacaag aatatgccat
     1381 gatgggtata tacggcgcgt ggaatctgcg ttcgccatca tcgccgagcc gaaccggcgg
     1441 gccatcctga gcctgctcgc ctcgacggag cgatccgtcg gcgagatcga gcggcagctc
     1501 cacatgtcgc agccctcggt ctcgaagcac ctccggatcc tgcgcgacgc ggggttcgtc
     1561 gaatcgcgcg tcgacgcgca gcgccgcgtc taccgcattc gaccg
//
LOCUS       DASVOR010002322         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_114013,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002322 DASVOR010000000
VERSION     DASVOR010002322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114013"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1650
                     /locus_tag="VF516_42815"
     CDS             <1..>1650
                     /locus_tag="VF516_42815"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEX8114560.1"
                     /translation="EAPEAGSRIALVDVHADAADAAVAVAWAAYNPSRYWALQRAAPQ
                     EDRDGWVDQKQYVYETSPGEHRMVMANAARHGGMWTVTLFDIDQAIAEKRAGQVALVL
                     GRLLPRDYQRETFAGKTAHPLDAERVAALTAFVDDSREALGVPGIALGLLQDGKVVFA
                     DGFGSRELGKAGKPDADTLFMIASNTKALTTLLLARLVDGKRLTWDTPVTRLMPSFKL
                     GDEATTSSVLVKHLICACTGLPRQDLPWLMEFKGATPASAMKLLGTMQPTSKFGEMFQ
                     YSNLLAAAAGYVGAYVIAPRKELGAGYDAAMAAEVFGPLGMTSTTFDYARALRGNHAG
                     AYGFDLDGAPAPALMAVNYAAIPVRPAGGAWSNVRDLLKYVAMELARGRLPGGKRYLG
                     EDALLARQAPQVAMSKDQSYGMGLMVDRTWGIQVVHHGGDLLGYHSDMIWLPEHNVGA
                     VLLTNSQAGTILINAFRRKLLEVLFDGRPVADGELAAAGRELRQAIASERERLTVPAE
                     DAAVGALAAHYHSDALGDIAVVRDRGITYFDVGEWRSPVGSR"
BASE COUNT          239 a          620 c          581 g          210 t
ORIGIN      
        1 ggaggcgccc gaggccgggt cgcggatcgc gctggtcgac gtccacgccg acgcggccga
       61 cgccgcggtc gcggtcgcct gggcggccta caacccgtcg cgctactggg cgctccagcg
      121 cgccgcgccg caggaggacc gcgacggctg ggtcgatcag aaacagtacg tctacgagac
      181 ctcgcccggc gagcaccgca tggtcatggc caacgccgcg cgccacggcg ggatgtggac
      241 ggtcacgctg ttcgacatcg accaggcgat cgccgagaag cgcgccggcc aggtcgccct
      301 ggtcctcggc cgcctgctgc ccagggacta ccagcgcgag acgttcgccg gcaagaccgc
      361 ccacccgctc gacgccgagc gggtcgccgc gctgaccgcg ttcgtcgacg actcgcgcga
      421 ggcgctcggc gtgcccggca tcgcgctggg ccttctgcag gacggcaagg tcgtgttcgc
      481 cgacgggttc ggcagccgcg agctcggcaa ggcgggcaag cccgacgccg acacgctgtt
      541 catgatcgcg tccaacacca aggcgctgac cacgctgctg ctcgcgcggc tggtcgacgg
      601 caagcggctg acctgggaca cgccggtcac ccggctgatg ccgtcgttca agctcggcga
      661 cgaggccacc acgtcgagcg tcctggtcaa gcacctgatc tgcgcgtgca ccggcctgcc
      721 gcgccaggac cttccgtggc tgatggagtt caagggcgcg accccggcga gcgcgatgaa
      781 gctgctcggc acgatgcagc cgaccagcaa gttcggcgag atgttccagt actccaacct
      841 gctcgccgcc gccgccggct acgtcggggc ctacgtgatc gcgccgcgca aggagctcgg
      901 cgcgggctac gacgccgcga tggctgccga ggtgttcggc ccgctcggca tgacgtcgac
      961 cacgttcgac tatgcccgcg cgctgcgcgg caaccacgcc ggcgcctacg gcttcgacct
     1021 cgacggcgcg cccgcgcccg ccctgatggc ggtcaactac gcggcgatcc cggtccgccc
     1081 ggccggcggc gcgtggagca acgtgcgcga cctgctgaag tacgtcgcga tggagctcgc
     1141 gcgcggcagg ctgcccggcg gcaagcgcta cctcggcgag gacgccctgc tcgcgcgcca
     1201 ggcgccccag gtcgcgatga gcaaggacca gagctatggc atgggcctga tggtcgaccg
     1261 cacctggggc atccaggtgg tccaccacgg cggcgacctg ctcggctacc acagcgacat
     1321 gatctggctg cccgagcaca acgtcggcgc ggtcctcctc accaactcgc aggccggcac
     1381 catcctcatc aacgcgttcc gccgcaagct gctcgaggtc ctgttcgacg gccggccggt
     1441 cgccgacggc gagctcgccg cggccggccg cgagctgcgc caggcgatcg ccagcgagcg
     1501 cgagcggctc accgtccccg cggaggacgc cgccgtcggc gcgctcgccg cgcactacca
     1561 cagcgacgcg ctcggcgaca tcgcggtcgt gcgcgaccgg ggcatcacct acttcgacgt
     1621 cggggagtgg aggagcccgg tcggctcgcg
//
LOCUS       DASVOR010002323         1705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_346132,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002323 DASVOR010000000
VERSION     DASVOR010002323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346132"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VF516_42820"
     CDS             complement(<1..952)
                     /locus_tag="VF516_42820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEX8114561.1"
                     /translation="MAPPDGEIRMPEIVQEALSRIQVLLDMLFETAEDAIFLSGDDGR
                     FVDCNPVALRMFGCATKDDVLGKTPVRFSPDRQPDGASSADKARELIAAALAGVPQHF
                     DWRHSRLDGAPFDVEVSLNRCFVGGASFLLGVVRDITSRKRAEAALLQEKQFSDRLID
                     SLPGTFYLFDPGLGLRRWNKNHETQMGYTAEELRGKRMVDLLPTEDERERVLAATRRL
                     MEHGGVETLETLVLHKDGSSVPYLVSGVRIDSPSGPMLMGAGIDISARVRAEKALAAS
                     ERNYRELFNATNEGIFIHDATGRVLDINERGCAMFGLDTSE"
     gene            complement(1089..>1705)
                     /locus_tag="VF516_42825"
     CDS             complement(1089..>1705)
                     /locus_tag="VF516_42825"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8114562.1"
                     /translation="PQRRALEHALPARWERRSGTPTARYVASLEGGVDGFLSRRSREL
                     RKSLRKSLRAAAQAGVTFESVKQVPADAAPALYARIQEVEATSWKAREGVGISGGPMR
                     AFYEHMLPRLCERGQQRILFARAADRDIGYILGAVFEGEYRGLQFSYDDAHAALGIGG
                     LLQYHQIVELCEEGVTRYDLGTEMDYKRRWAEEVMETEMLVLVR"
BASE COUNT          257 a          615 c          573 g          260 t
ORIGIN      
        1 cctcggaggt gtcgaggccg aacatggcac agccgcgctc gttgatatcc agcacgcggc
       61 cggtagcgtc gtggatgaag atcccctcgt tggtggcgtt gaacagctcc cggtagttgc
      121 gctcgctcgc tgcgagcgcc ttctcggccc ggacgcgcgc ggagatgtcg atgccggccc
      181 ccatgagcat gggaccgctc ggcgagtcga tgcgcactcc cgacaccagg tacgggacgc
      241 tggagccgtc cttgtgcaac accaaggtct ccagggtctc gaccccgccg tgctccatca
      301 gacggcgcgt cgccgcgagc acccgttccc gctcatcctc ggtcggcaag aggtccacca
      361 tccgcttgcc tcgcagctcc tcggccgtgt accccatctg ggtctcgtgg ttcttgttcc
      421 agcggcgcag accgagtccc gggtcgaaga gatagaatgt gccgggaagg gaatcgatga
      481 gacggtcgga gaactgcttc tcctggagca gcgccgcttc cgctcgcttg cgcgaggtga
      541 tgtcgcgcac caccccgagc aggaacgagg cgccgcccac gaagcaacga ttcaggctga
      601 cctcgacgtc gaacggcgct ccatcgagcc ggctatgtcg ccagtcgaag tgctggggaa
      661 cgcccgccag cgccgctgcg atgagctcgc gggccttgtc ggccgacgaa gcaccatcgg
      721 gctggcggtc cggcgagaac cggaccggcg ttttccccag gacgtcatcc ttggtcgcgc
      781 atccgaacat ccgcaaggcg acggggttgc agtccacgaa ccgcccgtcg tcccccgaga
      841 gaaagatcgc atcctcggcc gtctcgaaca gcatgtcgag caagacctgg atccgggaga
      901 gcgcctcctg gacgatctcc ggcatcctga tctcgccgtc cggcggcgcc atggccgtac
      961 tcgggtgcac gacaggtgcc agatcgcacc agatcgcccc ggcgcgcgtt cgcgcctgcg
     1021 gcgcgggtca gagacgttcc gggcgctcgg ggcgctggcg ccgatcgtgc tccggcgcgc
     1081 cgaggctgtc atcgcaccag gacgagcatc tcggtctcca tgacctcctc ggcccagcgc
     1141 cgcttgtagt ccatctcggt gccgaggtcg tagcgggtca cgccctcctc gcacagctcc
     1201 acgatctggt ggtactggag caggccgccg atgccgagcg cggcgtgcgc atcgtcgtag
     1261 gagaactgca gaccgcggta ctcgccctcg aacaccgcgc ccaggatgta gccgatatcg
     1321 cgatcggccg cgcgcgcgaa caggatgcgc tgctggccgc gctcgcacag ccggggcagc
     1381 atgtgctcgt agaacgcccg catcgggccg ccggagatcc cgacgccctc gcgcgccttc
     1441 cagctcgtcg cctcgacctc ctggatccgg gcgtagagcg cgggcgcggc gtcggccggc
     1501 acctgcttca ccgactcgaa ggtcacgccg gcctgggccg ccgcgcgcag cgacttgcgc
     1561 agcgacttgc ggagctcgcg cgaccggcgc gacaggaagc cgtcgacccc gccctccagg
     1621 ctcgcgacgt agcgcgccgt gggcgtgccg ctccggcgct cccagcgcgc cggcaaggcg
     1681 tgctccaggg cgcggcgctg tgggc
//
LOCUS       DASVOR010002324         1570 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_376112,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002324 DASVOR010000000
VERSION     DASVOR010002324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1570
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376112"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..764
                     /locus_tag="VF516_42830"
     CDS             201..764
                     /locus_tag="VF516_42830"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HEX8114563.1"
                     /translation="MAHGPAGAIVALESCGALGWCRITTTRRQRWLDTLSRCAMAASG
                     GVLVWPTIAGQQDLGLQSWCAGTPGVALALLTCFRLTKQPAYLELALDALEGMKALAR
                     NMFFSRTLCCGNAGYRHIFLEAYRVTKNAVWLDHAAAEARFSTSVKPWPRLGLYQGEL
                     GIAYLADRLAAPLAYPLPALGIISSAG"
BASE COUNT          240 a          541 c          529 g          260 t
ORIGIN      
        1 tagaacacgc tcggctgcag gttctcctcg ttgcacaagt ccgacaccgg gaccttgtcg
       61 accatgtggc gcttgaggat cgcgaccttc tgctcggtgg tgaagtggcg gcgggaacgc
      121 ttggaactcg acatcggtct cttggcagcc ggtgcgtacc acggcggtct gaccgtcacg
      181 ttccagccga ggcagaacaa atggcgcacg gcccggcagg agccatcgtc gcgctcgagt
      241 cgtgcggcgc gctgggatgg tgtcgtatca ccaccacgcg gcggcagcgc tggctggata
      301 cgctctcccg gtgcgccatg gccgcatccg gcggggtcct ggtatggcca acgatcgcgg
      361 gacagcagga cctgggcctg cagtcctggt gcgcaggtac gcccggggtc gcgctggcgc
      421 tgctcacgtg cttccgcctg accaagcagc cggcctacct cgagctcgcg ctagacgcgc
      481 tcgagggcat gaaagccctg gcgcgaaaca tgttcttctc gaggacgctg tgctgcggga
      541 acgccggcta tcgacatatc tttctcgagg cctatcgcgt cacgaagaat gccgtgtggc
      601 tcgatcacgc cgcggcggag gcgcgattct cgacgtccgt caagccttgg ccgcggctcg
      661 gcctgtacca gggcgagctc ggcattgcct atctggcgga tcggcttgcg gccccgctag
      721 cctatccatt gcccgcgttg ggaatcatat ccagtgctgg ctgatcaaca ccatcacggc
      781 gaggagcagc ttcaaccgat ccacgaggtg gagcgtcggg cgcttgcagc agcgccagag
      841 gacgttgacc tggtggttct cgctcgccga cttccgcggg ccagctgcga ctacagctga
      901 ccacgcgcgc caggacccac atccggattc gaggacacct ggcaagcgca tccctcactc
      961 ggagcacgaa acgaggtcgt tgtagctgtc gcagtgccgc gccgccacgt cctcgaacgt
     1021 cctggccgcg tcgccgtcga acgtgctgac ctcgtcctcg ctgctggtcg ccgggctgcc
     1081 atcctggctc gccgggccgt gctggctcgc cgggcttcgc gccggtgacg acggcgatca
     1141 cagggtcgag gacggcaacg cccccacgtg ggtgccagcc accaccgccg gcccggccgc
     1201 tgccggctgc accgctctcg ccgtgccagt cgtcgagggg gccgaccacg attccgcgga
     1261 tccgcggaat cgcccggcct gcgacctcat ggctgccacc tcggtgcctg ccagcaccgc
     1321 cgcgggatga cgacgatcgc accgcgggtt ccatcggtcg tcgacccggc cggcgccgag
     1381 cagcgacgcg gggagcgcct gggcacgtca ttcgtccggc ctggatcgcc ggctgctctg
     1441 cgctgcggta caatcgggtc gtgttcacgg atccggccac catgaccgaa gaacagcgcc
     1501 agtgggtgga gctgagccgg aagctgtggc gacgtgcgga acgcatcgca gcccagcatc
     1561 cgggcatgga
//
LOCUS       DASVOR010002325         6425 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_521689,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002325 DASVOR010000000
VERSION     DASVOR010002325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521689"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF516_42835"
     CDS             complement(<1..405)
                     /locus_tag="VF516_42835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEX8114564.1"
                     /translation="MPTLPRRVLLGLYIAFLVIPLYWLVVMSLKTNEEILGELSLFPR
                     HPTLANYLTIVTDRSWYMGYVNSLEYVVMNTALSLAVALPAAYAFSRYQFVGDKHLFF
                     WLLSNRMSPPAVFLLPFFQLYSSVGLFDTHIAV"
     gene            complement(483..1841)
                     /locus_tag="VF516_42840"
     CDS             complement(483..1841)
                     /locus_tag="VF516_42840"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8114565.1"
                     /translation="MIDDETGTTTEQIDRQAPFGDRVLLVIDGAALQVVPLPAAGTLT
                     IGRASKCDVVIDSGSVSRHHANLAIATEIEIEDVGSSNGTFVDGVRLTPRQPVQLTVG
                     VPFLLGAVTVMVQARTATGRPAATRSSALAALEQSASRIALGRLSVLVVGELGVGKER
                     FAERIHEMSPRRTASFVRINCSAISEPALEAELFGNEAANKPGALELAEGGTAFLADV
                     DQLPQMLQHKLLRAIEDAAVRRVGATRARPIDVRYVASTAKNLSSEIDDGRFRRDLYF
                     RLAGATFTIPPLRNRRDEILPLAEQFVAAAAAPLGRSFAFSEDAKQWLADHGWTGNIR
                     ELRNACERAVLLATGSLIESQHLTTVNDPVQRPSQRTRSSATIPPPPGIRAPSAADMP
                     TQVRATVAELEKQRILEALERCAGNQTRAAEMLGISRRTLINRLDEYGIARPRKRDDP
                     QT"
     gene            complement(1956..2456)
                     /locus_tag="VF516_42845"
     CDS             complement(1956..2456)
                     /locus_tag="VF516_42845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114566.1"
                     /translation="MSTFQLKKISSDGIPHAMEKAERYRLLNDPPQAESICLDVLAVD
                     PDNQDALRCLILSLTDQFGASGAAHTAREARTYIARLADEYERAYYNGLVHERETRAF
                     LHRKNVVRSAAYDGFRHAMEWYERAEALRPPGNVDAILRWNSCARAIERERLSPAEDS
                     HELPLE"
     gene            complement(2521..3387)
                     /locus_tag="VF516_42850"
     CDS             complement(2521..3387)
                     /locus_tag="VF516_42850"
                     /inference="COORDINATES: protein motif:HMM:NF009052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEX8114567.1"
                     /translation="MAKRVFKVWRGNAQGGDFKQFDIDVDPGMVVLDVLHRIQAQQAN
                     DLALRWNCKAGKCGSCSMEINGKPRLACMTRMNSFGEDETITCQPLKTFPVMKDLVTD
                     VSWNYEQNKKIPKFKPRPREADGTYRMYQEDVDRVQEFRKCIECFLCQDVCHVLRDRE
                     KAPQHARAGDKVKGAFVGPRFMIRLASLEMHPLDTDDRIPEIRKEFGSGMCNITRCCT
                     EVCPESIGITDNGIIPLKERVVDRYFDPITVIGRKLFGIRRRTKARSEEAGAAPAAPA
                     PGSAAREGKGPA"
     gene            complement(3413..5257)
                     /locus_tag="VF516_42855"
     CDS             complement(3413..5257)
                     /locus_tag="VF516_42855"
                     /inference="COORDINATES: protein motif:HMM:NF005866.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEX8114568.1"
                     /translation="MLDVSEIQTIEHDVVVVGAGGAGLRAAIECSAHKLSTGVLSKSL
                     LGKAHTVMAEGGMAAAMGNVDSRDNWKVHFRDTMRGGKFLNVWRMAELHAKQAPDRVR
                     ELEEWGAVFDRTKEGLISQRNFGGHRYPRLAHVGDRTGLELIRSLQDHGVHQGISFHM
                     ECTALEVLKDGDRVAGLLAYWRETGRFVVFRCKAVIFATGGGGKAWPVTSNSWEYTGD
                     GIAMAYEAGAELMDLEFTQFHPTGMVWPLSVRGILVTEGVRGDGGVLKNNKGERFMFN
                     YISERFAPETAATIEEGDRWLAGDKSARRPPELLTRDEVARAITAEVKAGRGSPHGGV
                     FLDIASRRPADFIKRKLPSMYHQFKELAEVDITREPMEVGPTLHYFMGGIRVDADTQE
                     STVPGLFACGECGAGMHGANRLGGNSLSDLVVFGKLAGDGAKQYISALGETPKPSSDQ
                     ITAGIRRATEILNRESGANPYVLHDKLHGVMSRGVGIVRKKDELEQAVRELDELKAEA
                     ATMRAPGTSQYNPGWHEALSMRALLVTSEAVTRAALVREESRGAHTRLDFPGESPEWV
                     KYNVIIKKGADGMEVEKRLRPDGPAHLVAIANAKIEDLESGKVGADVK"
     gene            complement(5263..6093)
                     /locus_tag="VF516_42860"
     CDS             complement(5263..6093)
                     /locus_tag="VF516_42860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114569.1"
                     /translation="MATHLPVIGGHAEGFAKTARTDDWKSGPAITFVVFLTFVVYTTW
                     AALQGNHYYYDPYLSPFYSPVLFSDPTVAGAAPVSHAWFGAVPSWWPSKLISFSPALL
                     ILVFPGSFRFTCYYYRKAYYRAFAGSPPGCAVGPLAGGRKYRGETAILLFQNLHRYAL
                     YFALAFIVILGKDAVEAFFKDGRPGIGVGSIILTINVLLIASYTFGCHSFRHLIGGRK
                     DCMSDCGRPTMALGGWKRASWFNARHMQFAWASLIWVMVTDVYVRLASIGVIHDFNTW
                     "
     gene            6212..>6425
                     /locus_tag="VF516_42865"
     CDS             6212..>6425
                     /locus_tag="VF516_42865"
                     /inference="COORDINATES: protein motif:HMM:NF042696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HEX8114570.1"
                     /translation="MPPAHELPYTEIAPPGDLAPYVDRLWLRTTLRAPARVHRVLPDG
                     CADVIVDAGRGAAELVGPMTRAIEVAE"
BASE COUNT          908 a         2228 c         2285 g         1004 t
ORIGIN      
        1 gaccgcgatg tgggtatcga acagcccgac gctggagtac agctggaaga acggcaagag
       61 gaacaccgcg ggcggcgaca tccggttgga cagcagccag aagaacaggt gcttgtcgcc
      121 gacgaactgg tagcgcgaga acgcgtacgc cgcgggcagc gccaccgcga gcgacagcgc
      181 ggtgttcatc accacgtact cgagcgagtt gacgtagccc atgtaccacg accgatcggt
      241 gacgatggtc aggtagttcg ccagcgtggg atggcgcggg aacaggctga gctcgccgag
      301 gatctcctcg ttggtcttga gcgacatcac cacgagccag tacagcggga tcaccaggaa
      361 ggcgatgtac aacccgagca ggacgcggcg ggggagcgtg ggcatgcgcg cgagctttcg
      421 gcgcgaatca tcgccacgcc gcgccgcggg gcgcaagcgg accggccagc tcgctttgcg
      481 ggtcaggttt gcgggtcgtc gcgcttgcgc ggccgggcga tgccgtactc gtcgagccgg
      541 ttgatcagcg tgcggcgcga gatcccgagc atctcggcgg cccgggtctg gttgccggcg
      601 cagcgctcga gcgcctcgag gatccgctgc ttctcgagct cggcgacggt ggcgcggacc
      661 tgggtcggca tgtcggcggc gctcggcgcc cggatcccgg gcggcggcgg gatcgtcgcc
      721 gagctgcggg tgcgctggct gggccgctgg accgggtcgt tgaccgtcgt caggtgctgg
      781 ctctcgatca gcgagccggt ggcgagcagc accgcgcgct cgcacgcgtt gcgcagctcg
      841 cggatgttgc cggtccagcc gtgatcggcg agccactgct tggcgtcctc ggagaacgcg
      901 aacgaccggc cgagcggcgc cgcggcggcg gcgacgaact gctcggcgag cggcaggatc
      961 tcgtcgcggc ggttgcgcag cggcgggatc gtgaacgtcg cgccggcgag ccggaagtac
     1021 aggtcgcggc ggaaccggcc gtcgtcgatc tcggacgaca ggttcttggc ggtcgaggcg
     1081 acgtagcgca cgtcgatcgg ccgggcccgg gtcgcaccga cccggcggac cgcggcgtcc
     1141 tcgatcgcgc gcagcagctt gtgctgcagc atctggggca gctggtcgac gtcggcgagg
     1201 aacgcggtgc cgccctcggc gagctccagc gcgccgggct tgttggccgc ctcgttgccg
     1261 aacagctcgg cctccagcgc cggctcgctg atcgcgctgc agttgatccg gacgaacgag
     1321 gcggtccggc gcggcgacat ctcgtggatc cgctcggcga accgctcctt gccgaccccg
     1381 agctcgccga ccaccagcac gctgagccgg ccgagcgcga tccgcgacgc cgactgctcg
     1441 agcgcggcca gcgcgctcga cctggtcgcc gccgggcggc cggtggcggt ccgcgcctgg
     1501 accatcacgg tgaccgcgcc gagcaggaac ggcacgccga ccgtgagctg gaccggctgg
     1561 cgcggggtca gccggacgcc atcgacgaac gtgccgttcg agctgccgac gtcctcgatc
     1621 tcgatctcgg tggcgatcgc gaggttggcg tggtggcgcg acaccgaccc cgagtcgatc
     1681 acgacgtcgc acttgctggc ccggccgatc gtcagcgtgc cggccgcggg cagcggcacg
     1741 acctgcagcg cggcgccgtc gatcacgagc agcacgcggt caccaaacgg cgcctggcga
     1801 tcgatctgct cggtcgtcgt gccggtctcg tcgtcgatca cccgggtagg gtacgcggag
     1861 ccggcggcgt cgcgcaaccg cagcccccct ccgcggcgcc cggacggccg ggacgcggaa
     1921 tgaccgcgca acatggcgcg gcagcggctg ccgccctact ccagcggcag ctcgtggctg
     1981 tcctcggcag gggacagccg ctcgcgctcg atcgcgcggg cgcagctgtt ccagcgcagg
     2041 atcgcgtcga cgttgcccgg cgggcgcagc gcctcggcgc gctcgtacca ctccatcgca
     2101 tgccggaacc catcgtaggc cgccgaccgg acgacgttct tgcggtgcag gaacgcgcgg
     2161 gtctcgcgct cgtggaccag gccgttgtag taggcgcgct cgtactcgtc ggccagcctg
     2221 gcgatgtagg tccgggcctc gcgcgcggtg tgggcggccc cgctggcgcc gaactggtcg
     2281 gtcagcgaca ggatcaggca gcgcagcgcg tcctggttgt cggggtcgac cgcgagcacg
     2341 tccaggcaga tgctctcggc ctgcggggga tcgttgagca gccggtagcg ctcggccttc
     2401 tccatcgcgt gggggatgcc gtcggaggag atcttcttga gctggaacgt gctcatgggc
     2461 cgagcttagc ccggcgcggc gccgccatca cggccgggcc gtggggtcac tttcagtcgg
     2521 tcaggccggg cccttgccct cgcgcgccgc gctcccgggc gccggagctg ccggggccgc
     2581 gcccgcctcc tcgctgcggg ccttggtgcg ccggcggatg ccgaacagct tgcggccgat
     2641 gacggtgatc gggtcgaagt agcgatcgac cacccgctcc ttgagcggga tgatgccgtt
     2701 gtcggtgatg ccgatcgact cggggcagac ctcggtgcag caccgggtga tgttgcacat
     2761 ccccgagccg aactccttgc ggatctcggg gatccggtcg tcggtatcca gcgggtgcat
     2821 ctccaggctg gcgagccgga tcatgaaccg gggcccgacg aacgcgccct tgaccttgtc
     2881 accggccctg gcgtgctgcg gcgccttctc gcggtcgcgc agcacgtggc agacgtcctg
     2941 gcacaggaag cactcgatgc acttgcggaa ctcctgcacc cggtcgacgt cctcctggta
     3001 catccggtag gtcccgtcgg cctcgcgcgg ccgcggcttg aacttgggga tcttcttgtt
     3061 ctgctcgtag ttccacgaca cgtcggtgac gaggtccttc atcaccggga acgtcttgag
     3121 cggctggcag gtgatggtct cgtcctcgcc gaagctgttc atccgcgtca tgcaggccag
     3181 ccgcggcttg ccgttgatct ccatcgagca cgagccgcac ttgccggcct tgcagttcca
     3241 gcgcagcgcg aggtcgttgg cctgctgggc ctggatccgg tgcagcacgt cgagcacgac
     3301 catgccgggg tcgacgtcga tgtcgaactg cttgaagtcg ccgccctggg cattgccgcg
     3361 ccagaccttg aagactcgct ttgccattgc attgactcct tgggcgaacc gactacttga
     3421 cgtccgcgcc gaccttgccc gactcgagat cctcgatctt ggcgttggcg atcgccacga
     3481 ggtgggccgg gccgtcgggc cggagccgct tctcgacctc catgccgtcc gcgcccttct
     3541 tgatgatcac gttgtacttg acccactcgg ggctctcgcc ggggaagtcg agccgggtgt
     3601 gggcgccgcg gctctcctcg cgcaccagcg cggcgcgggt caccgcctcg gaggtgacca
     3661 gcagcgcgcg catcgacagc gcctcgtgcc agcccgggtt gtactggctg gtgcccggcg
     3721 cgcgcatcgt cgccgcctcg gccttgagct cgtcgagctc gcgcaccgcc tgctcgagct
     3781 cgtccttctt gcgcacgatc ccgacgccgc ggctcatcac gccgtgcagc ttgtcgtgca
     3841 gcacataggg gttggcgccg gactcgcggt tcaagatctc ggtcgcgcgg cggatcccgg
     3901 cggtgatctg gtcgctggac ggcttcggcg tttcgcccag cgccgagatg tactgcttgg
     3961 cgccgtcgcc cgcgagcttg ccgaacacga ccaggtcgga cagcgagttt ccgccgagcc
     4021 ggttggcgcc gtgcatgccg gcgccgcact cgccgcatgc gaacagcccg ggcaccgtgc
     4081 tctcctgggt gtcggcgtcg acccggatgc cgcccatgaa gtagtgcagc gtcgggccga
     4141 cctccatcgg ttccctggtg atgtcgacct cggccagctc cttgaactgg tggtacatcg
     4201 acggcagctt gcgcttgatg aagtccgccg gccggcgcga cgcgatgtcg aggaacacgc
     4261 cgccgtgcgg cgagccgcgg ccggccttga cctcggcggt gatcgcgcgg gcgacctcgt
     4321 cgcgggtgag cagctcgggc ggccgccgcg ccgacttgtc gccggcgagc cagcggtcgc
     4381 cttcctcgat cgtggcggcg gtctccggcg cgaaccgctc ggagatgtag ttgaacatga
     4441 accgctcgcc cttgttgttc ttgagcacgc cgccgtcgcc gcgcacgccc tcggtgacca
     4501 ggatgccgcg caccgacagc ggccacacca tgccggtcgg gtggaactgg gtgaactcga
     4561 ggtccatcag ctcggcgccg gcctcgtagg ccatcgcgat gccgtcgccg gtgtactccc
     4621 aggagttcga cgtcaccggc cacgccttgc cgccgccgcc ggtcgcgaag atcaccgcct
     4681 tgcagcggaa caccacgaac cgcccggtct cgcgccagta cgcgagcagc ccggcgaccc
     4741 ggtcgccgtc cttgagcacc tcgagcgcgg tgcactccat gtggaagctg atgccctggt
     4801 ggacgccgtg gtcctgcagc gagcggatca gctcgaggcc ggtgcggtcg ccgacgtggg
     4861 cgagccgcgg gtagcggtgg ccgccgaagt tgcgctgcga gatcagcccc tccttggtgc
     4921 ggtcgaacac cgcgccccac tcctcgagct cgcgcacgcg atccggcgcc tgcttggcgt
     4981 gcagctcggc catccgccac acgttgagga acttgccgcc gcgcatcgtg tcccggaagt
     5041 ggaccttcca gttgtcgcgc gagtcgacgt tgcccatcgc cgccgccatg ccgccctcgg
     5101 ccatcacggt gtgggccttg ccgagcaggc tcttggacag gacgccggtg gagagcttgt
     5161 gcgcgctgca ctcgatcgcc gcgcgcagcc cggcgccgcc cgcgccgacg accaccacgt
     5221 cgtgttcgat ggtctggatc tcggatacgt cgagcatgct ggtcaccagg tgttgaaatc
     5281 gtggatcacg ccgatcgagg ccagccgcac gtagacgtcg gtgaccatga cccagatcag
     5341 gctggcccac gcgaactgca tgtggcgcgc gttgaaccag ctcgcccgct tccagccgcc
     5401 gagcgccatg gtcggccggc cgcagtccga catgcagtcc ttgcggccgc cgatcaggtg
     5461 gcggaacgag tggcaaccga acgtgtagct cgcgatcaac agcacgttga tcgtgaggat
     5521 gatgctgccg accccgatcc cgggcctgcc gtccttgaag aaggcttcga ccgcgtcctt
     5581 gccgaggatg acgatgaagg ccagcgcgaa gtacagcgcg tagcggtgca ggttctggaa
     5641 cagcaggatc gccgtctcgc cgcggtactt gcggccaccg gcgagcggcc cgaccgcgca
     5701 ccccggcggc gagccggcga acgcgcgata gtacgccttg cggtagtagt aacaggtgaa
     5761 ccggaagctg ccggggaaca ccaggatcag cagcgccggc gagaacgaga tcagcttgga
     5821 cggccaccag ctcggcaccg cgccgaacca cgcgtggctg accggcgccg cgccggcgac
     5881 tgtcggatcg ctaaacagca ccggcgagta gaacggcgac aggtacgggt cgtagtaata
     5941 gtggttgccc tggagcgccg cccacgtggt gtagacgacg aacgtcagga agacgacgaa
     6001 cgtgatcgcg gggccgctct tccagtcgtc ggttcgcgcc gtcttcgcga acccttccgc
     6061 gtgcccaccg atcacgggca ggtgtgtggc catgtcgcgg cggactatag ggaaaccgac
     6121 ctggcgccgc aagcgaaggg gccgccgcaa atgccgcgct ggcgcccggc gcacggttct
     6181 cgcccgcgcc gtcccgtgcg acgcttgccg gatgccaccc gcgcacgagc tgccgtacac
     6241 cgagatcgcg ccaccgggcg atctcgcccc gtacgtcgat cgcctgtggc tgcggaccac
     6301 gctgcgcgcg ccggcgcgcg ttcaccgcgt gctccccgac ggctgcgccg acgtgatcgt
     6361 cgacgccggg cgcggcgccg ccgagctggt cggcccgatg acccgggcga tcgaggtcgc
     6421 ggagg
//
LOCUS       DASVOR010002326         3671 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_521703,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002326 DASVOR010000000
VERSION     DASVOR010002326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_521703"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..1433
                     /locus_tag="VF516_42870"
     CDS             174..1433
                     /locus_tag="VF516_42870"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8114571.1"
                     /translation="MPAGLRDALVIARREFVERVRSRWFVVITLLGPLGMIALVLVPA
                     LLVGRGVEGSRIEIVDRSGVLGQPLSDELARERWKPTIVPGDTAKADEMARIRDQKIN
                     GFITIPDSWLDNGSTGQIVYLGDNASNEGVERTLRNAVQRVVQGERGRRAHIPDQTLG
                     MVLQKANFTAQHTNGETEGASGMASFVIAYLLAFILYMVIALYGVNVMRSVVQEKTTR
                     VMELMVATVKPRSLMTGKILGVGGAGLVQIVAWLAMGAITLSYREQLLGWFGVSGGGP
                     ALPPIELGTIAVVVVYFVLGYFFYASMYAAVGAMVASEQDTQQVQMPITMLLLIGALC
                     LQMVSNAPRGSATVIMTMIPFWSPMLMPMRYVLGGATPVEVVISLAILLGSTVLVARA
                     AAKIYRTGVLMYGKRPTVQELLRWLRY"
     gene            1528..3081
                     /locus_tag="VF516_42875"
     CDS             1528..3081
                     /locus_tag="VF516_42875"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEX8114572.1"
                     /translation="MASLALPGALIVALAGSAAAQPADPPVDVTATGPDGAGPRPPQR
                     IGYGALPGGLHVASAEALPAGTVELGLLSGFGYRKGLLSATHTFDRAIGDVAVAYAPV
                     AHLAIALSLDGRYDRHQGSGTTDDGYVGDPHLLVRFAAPAGRVALGGQLGIWVPGKNA
                     PSIDASATSVDARALLSFDAGFGLITANAGFRYDNSAKSVDQPTALSAADQVSLGVSS
                     FSAALAGLSLRIPIHERSYVALEGSADVFVGNGAPGPIVRGGGLFGVAVNDAVAVIAY
                     VEGAKVPGLSYGDVMQKNLVTLVPYEPVITGGLGLQARFGGARPPAPTSQITRNARPV
                     PVTVIETADVTGSVVDDAGKPVVGAKITIKLKNNTATAISDDKGTFVVTRLPIGKTID
                     GKTDLDDTGAEISAEVANKKPGSATLTLAKGANIVPKIALDPMLPPGQLRAVIINVGT
                     SRPVAGATVTIEPGGATATSGPDGKFTVDLPPGHYKITVTAHGLAQQQLDVNIDPNGV
                     AIKNIDMHK"
     gene            3098..>3671
                     /locus_tag="VF516_42880"
     CDS             3098..>3671
                     /locus_tag="VF516_42880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114573.1"
                     /translation="MVAAMLVACRQGGDAIAELGEANGPVGRQEGSGAWQPARIGTRY
                     FLGDAARVEIGRATLHIVGATAQIAMLDGTVLRFGGKPGEIRISVEQGTIDLTGNGSV
                     SLDTGKVKLAGGTVEITSKGPGRSSVELTLGKGQVETNGQTFDLAIGQPREIGLPARP
                     PIDAGVAAPVPVAIDAAVDAAAEPTVGDATI"
BASE COUNT          538 a         1293 c         1308 g          532 t
ORIGIN      
        1 gctcgcggag ggcgcgagct cgcagcagct gctcgcggcg ctggtcgccg ccggggtccg
       61 cctgcagcgg ttcgaggtcg tcgtcccgac cctgcaccag atcttcgtcg accgcgtcgg
      121 agccagcgcc acggtcgccg agcgccggcc ggaggcgccc tgacatgccg ggcatgccgg
      181 ccgggctccg cgatgcgctg gtgatcgcgc gccgcgagtt cgtcgagcgc gttcgctcga
      241 ggtggttcgt ggtgatcacg ctgctcggcc cgctcggcat gatcgccctg gtcctggtgc
      301 cggcgctgct ggtcggccgc ggtgtcgagg gatcgcggat cgagatcgtc gatcgctccg
      361 gggtgctggg gcagccgctg tccgacgagc tcgccaggga gcgctggaag ccgacgatcg
      421 tcccgggcga cacggcgaag gccgacgaga tggcccggat ccgcgatcag aagatcaacg
      481 gcttcatcac gatcccggac agctggctcg acaacggttc gaccggccag atcgtctatc
      541 tcggggacaa cgcctcgaac gagggcgtcg agcggacgct gcgcaacgcg gtccagcggg
      601 tcgtgcaggg cgagcgcggc cgccgcgccc acatccccga ccagaccctg ggcatggtgc
      661 tgcagaaggc gaacttcacg gcgcagcaca ccaacggcga gaccgagggg gcctcgggga
      721 tggcgtcgtt cgtgatcgcc tacctgctgg cgttcatcct ctacatggtg atcgccctgt
      781 acggcgtcaa cgtgatgcgc agcgtggtcc aggagaagac cacgcgcgtg atggagctca
      841 tggtcgccac cgtcaagccg cggtcgctga tgaccggcaa gatcctcggg gtcggcgggg
      901 cggggctggt gcaaatcgtc gcctggctgg ccatgggcgc gatcaccctg agctatcgcg
      961 agcagctcct gggctggttc ggtgtgtcgg gcggcggccc cgccctaccg cccatcgagc
     1021 tcggcacgat cgcggtcgtg gtcgtgtact tcgtgctcgg ctacttcttc tacgcctcga
     1081 tgtatgccgc ggtaggagcg atggtcgcgt ccgagcagga cacgcagcag gtgcagatgc
     1141 cgatcacgat gttgctcctg atcggcgcgc tgtgcctgca gatggtcagc aatgctccgc
     1201 gcggctccgc gacggtaatc atgacgatga ttccgttctg gtcgccgatg ctcatgccga
     1261 tgcgctacgt gctcggcggg gcgacgccgg tcgaggtcgt gatctcgctc gcgatcctgc
     1321 tggggtcgac cgtgctggtc gcccgtgcag cggccaagat ctaccggacc ggcgtgctga
     1381 tgtacggcaa gcggccgacc gtccaggagc tgctgcggtg gctgcggtat tgaaaactgt
     1441 gtcagggtag caaatcgggt tgaaacccgc gtagaatttt cggcgatgcg ccggtggcga
     1501 ctgaccgcga cgtcacgctc tgggtcgatg gcatcgctcg cgctgccggg tgcgctgatc
     1561 gtcgcgctgg ccgggagtgc agccgcccag ccggccgatc cgcccgtcga cgtcaccgcc
     1621 acgggcccgg acggcgcggg gccgcgtccg ccccagcgca tcggctacgg cgcgctgccc
     1681 ggcgggctgc acgtcgcctc ggccgaggcc ttgccggcgg ggacggtcga gctcggcctg
     1741 ctcagcgggt tcggctatcg caagggcctg ctgtcggcga cccacacctt cgaccgcgcg
     1801 atcggcgacg tcgcggtcgc ctacgcgccg gtggcccacc tggcgatcgc gctgtcgctc
     1861 gacggccgct acgaccgcca ccagggctcc gggacgaccg acgatggcta cgtgggcgat
     1921 ccgcacctcc tggtccggtt cgcggcgccc gcgggtcggg tcgcgctcgg cggccagctc
     1981 gggatctggg ttcccggcaa gaacgccccg tcgatcgacg cctccgcgac gtcggtcgac
     2041 gcccgcgcgc tgctctcgtt cgacgccggg ttcggcctga tcacggccaa cgccgggttc
     2101 cggtacgaca acagcgccaa gtcggtcgac cagccgaccg cgctgtcggc cgccgatcag
     2161 gtctcgctcg gggtctcgag cttcagcgcg gcgctcgccg gcctgtcgct gcgcatcccg
     2221 atccacgagc gatcgtacgt cgcgctcgag ggctcggccg acgtgttcgt cggcaatggc
     2281 gcgccggggc cgatcgtgcg cggcggcggg ctgttcggcg tcgcggtcaa cgacgcggtg
     2341 gcggtgatcg cctacgtcga gggcgccaag gtccccggcc tgagctacgg cgacgtgatg
     2401 cagaagaacc tggtcaccct cgttccgtac gagccggtga tcaccggcgg gctcggactc
     2461 caggcgcggt tcggcggcgc ccggccgccc gcgccgacca gccagatcac ccgcaacgcc
     2521 aggccggtgc cggtcacggt gatcgagacc gccgacgtca ccggcagcgt ggtcgacgac
     2581 gccggcaagc cggtggtcgg cgccaagatc acgatcaagc tcaagaacaa caccgccacc
     2641 gcgatcagcg acgacaaggg caccttcgtg gtcacccggc tgccgatcgg caagacgatc
     2701 gacggcaaga ccgacctcga cgacaccggc gccgagatct cggccgaggt cgccaacaag
     2761 aagccgggct cggcgaccct gacgctggcc aagggcgcca acatcgtgcc caagatcgcg
     2821 ctcgatccga tgctgccgcc cggccagctg cgcgcggtga tcatcaacgt cggaaccagc
     2881 cggccggtcg ccggcgcgac ggtgaccatc gagccgggcg gcgccaccgc gacgtcgggc
     2941 cccgacggca agttcacggt ggacctcccg cccggccact acaagatcac cgtgaccgcg
     3001 cacgggctcg cccagcaaca gctcgacgtg aacatcgacc ccaacggtgt tgcgatcaag
     3061 aacatcgaca tgcacaagta gcctcggctg cgcgtgcgtg gtcgcggcga tgctcgtcgc
     3121 gtgccggcag ggcggagacg cgatcgccga gctcggcgag gccaatggcc cggtcggccg
     3181 ccaggagggc agcggggcgt ggcagccggc ccggatcggc acgcggtact tcctcggcga
     3241 cgccgcgcgg gtcgagatcg gccgcgccac cctccacatc gtcggcgcca ccgcgcagat
     3301 cgccatgctc gacggcaccg tgctgaggtt cggcggcaag ccgggggaga tccggatctc
     3361 ggtcgagcag ggcacgatcg atctcaccgg caacggcagc gtttcgctcg acaccggcaa
     3421 ggtcaagctc gccggcggca cggtcgagat cacctcgaag ggccccgggc gatcgagcgt
     3481 cgagctcacg ctcggcaagg gccaggtcga gaccaacggc cagaccttcg atctcgcgat
     3541 cggccagccg cgcgagatcg gccttccggc ccgtccgccg atcgatgccg gggtcgccgc
     3601 gccggtcccg gtcgcgatcg acgccgcggt cgacgccgcc gccgagccca ccgtcggcga
     3661 cgcgacgatc g
//
LOCUS       DASVOR010002327         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_317765,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002327 DASVOR010000000
VERSION     DASVOR010002327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317765"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="VF516_42885"
     CDS             <1..440
                     /locus_tag="VF516_42885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114574.1"
                     /translation="PAVAEPTGEPPVAVVEPAPTGSGSPASDPAPVGPGSLDAVPALD
                     KLDVRGSLASSVVKRSMERHLGSLRACYQTAARAGNTTPAVDLMVSLEIDENGAATHV
                     MTSGPGFGSLASCAAGVLGGIHTQQAPDTGTAQVSVLIRFRPS"
     gene            484..822
                     /locus_tag="VF516_42890"
     CDS             484..822
                     /locus_tag="VF516_42890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114575.1"
                     /translation="MKRTTPLHRTAVLAVLLAASCADAPRGLLTGAPNPPAEIQQDCA
                     VATAKCSRCHPIDRVVVARGIGVGKWQMYVEQMRLKPSSGISLRDADVIFQCLRFVED
                     SCTDCKQGRS"
     gene            819..1967
                     /locus_tag="VF516_42895"
     CDS             819..1967
                     /locus_tag="VF516_42895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114576.1"
                     /translation="MISSRRFLLAAAVLAAAAGPTSADTLERNFAGSIQLDYMAVPSE
                     SIGRKIGLDSATAEVSMKVTQDFGDNVSASIKLCVACHGIETGMAFFDLRIADELNFR
                     VGRFTPQFGSFPIRYDPANHRTSDKPLPYDMGRMLRFSEWNMGVLPSPWVDNGVELSG
                     AHYFTDDVRLDYAAYAVGGPRAQSGAADFDYRLSRSGDAYYVDNNSRPSLGGNAAINI
                     VAGKTTVTAGASGMFGTYDPDNKLHYQIAGAHLVLQLSRVFLRAEYLVRRTEMVLGDD
                     PAQRFKYGPGKNGRYDPYFLKSGWYAELELPAGDRLDVILRWDGLRRQGNVLRDSELR
                     SDSAVLRYTAGASVKVRGAVRIKLSGELYDFSDFPDEVAIHLALTGTL"
     gene            1970..2983
                     /locus_tag="VF516_42900"
     CDS             1970..2983
                     /locus_tag="VF516_42900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114577.1"
                     /translation="MEPIMQRVVHRLVAFAILGATTAGCRLGPLVPDDPGASANVLPK
                     DAVIPGVTSNRDLMNQIILNDSLDTDTLMKNNNVIVRGTTGSATDGTQVKFWAFGNAD
                     RAPTPIYVFGTGDPMSASFTPLTDHPPLVETVPGDIDYEPIHTIFNVKVTDKYAGQKI
                     TTLGALSDAIDLGLVEAPVAIKVFVNWPIVRPGLRLEVGTGATVPPTPAYAHGYVVDG
                     FPLGGMLGRQPNPRGLLPTSQVSFLREAGTPTFDRAHPIFQATIPTSPPGTSPNYTPV
                     SLVVYVDLVQPLKPTDIHKDSELFTRSASGDIVSADLSKVAQFTVTTKLTDLQIQFAE
                     GAP"
BASE COUNT          472 a         1133 c          945 g          440 t
ORIGIN      
        1 caccggccgt ggccgagccc accggcgagc cgccggtcgc cgtcgtcgag cccgcgccca
       61 cgggatccgg tagccccgcc agcgatcccg cgccggtcgg cccgggatcg ctcgacgcgg
      121 tgccggcgct ggacaagctc gacgtccggg gctcgctggc gagctcggtg gtcaagcgca
      181 gcatggagcg ccacctcgga tcgctgcgcg cctgctacca gaccgcggcg cgcgccggca
      241 acaccacgcc ggcggtcgat ctcatggtca gcctcgagat cgacgagaac ggcgccgcga
      301 cccacgtcat gaccagcggt cccgggttcg gttctctcgc gagctgcgcc gccggcgtgc
      361 tcggcgggat ccacacccag caggcaccgg acaccggcac ggcgcaggtc tccgtcctga
      421 tcaggttccg cccgtcgtga tcgcctctcc atgaccagcg cagatccgcc cactgttcct
      481 catatgaaac gaactacccc tctccaccgc accgcggtgc tggcggtgct gctcgccgca
      541 tcgtgcgccg acgccccgcg cggcctgctc accggcgccc ccaacccacc cgccgagatc
      601 cagcaggact gcgcggtcgc caccgccaag tgctcgcgct gccatccgat cgatcgggtg
      661 gtcgtggcgc gcgggatcgg cgtcggaaaa tggcagatgt acgtcgagca gatgcggctc
      721 aagccgtcga gcgggatctc gctgcgcgac gccgatgtga tcttccagtg cctccgcttc
      781 gtggaggact cgtgcaccga ttgcaagcaa ggccgatcat gatctcctcg cgccgtttcc
      841 tcctggccgc cgcggtgctc gccgccgccg ccggtcccac ctccgccgac accctggagc
      901 gcaacttcgc cggctcgatc cagctcgact acatggcggt gccctccgag tcgatcggcc
      961 gcaagatcgg gctcgactcg gcgaccgccg aggtgtcgat gaaggtcacc caggacttcg
     1021 gcgacaacgt gtcggcgagc atcaagctgt gcgtcgcgtg ccacgggatc gagaccggca
     1081 tggcgttctt cgacctgcgg atcgccgacg agctcaactt ccgggtcggc cggttcaccc
     1141 cgcagttcgg ctcgttcccg atccgctacg atccggccaa ccaccggacc agcgacaagc
     1201 cgctgcccta cgacatgggc cggatgctgc ggttctccga gtggaacatg ggcgtcctgc
     1261 cgtcgccgtg ggtcgacaac ggcgtcgagc tcagcggcgc ccattacttc accgacgacg
     1321 tccggctcga ctacgcggcc tacgcggtcg gcgggccgcg cgcgcagagc ggcgccgccg
     1381 acttcgacta ccggctgtcg cgctcgggcg acgcctacta cgtcgacaac aactcgcggc
     1441 cgtcgctcgg cggtaacgcc gcgatcaaca tcgtcgccgg caagaccacg gtgaccgccg
     1501 gcgcgtcggg gatgttcggg acctacgatc cggacaacaa gctgcactac cagatcgccg
     1561 gcgcccacct cgtgctccag ctgtcgcgcg tgttcctgcg cgccgagtac ctggtgcgcc
     1621 ggaccgagat ggtgctcggc gacgatccgg cgcagcgctt caagtacggc ccgggcaaga
     1681 acggcaggta cgatccgtac ttcctcaagt cgggctggta cgccgagctc gagctgcccg
     1741 ccggcgaccg gctcgacgtc atcctgcgct gggacgggct gcgccgccag ggcaacgtgc
     1801 tgcgggacag cgagctgcgg tcggacagcg cggtgctgcg ctacaccgcc ggcgcgagcg
     1861 tcaaggtccg cggcgcggtc cggatcaagc tcagcggcga gctctacgac ttcagcgact
     1921 tccccgacga ggtcgcgatc cacctcgccc tcacggggac cctctgacca tggagccgat
     1981 catgcagcgc gtcgttcatc gcctcgttgc cttcgcgatc ctcggtgcca ccaccgccgg
     2041 ctgccggctc ggtccgctgg ttccggacga ccccggcgcg agcgcgaacg tcctgcccaa
     2101 ggacgcggtg atccccggcg tcaccagcaa ccgcgatctg atgaaccaga tcatcctcaa
     2161 cgacagcctc gacaccgaca cgctgatgaa gaacaacaac gtcatcgtgc gcggcaccac
     2221 cgggtccgcc acggacggca cgcaggtcaa gttctgggcg ttcggcaacg ccgatcgcgc
     2281 gccgaccccg atctacgtgt tcggcaccgg cgacccgatg tcggcgtcgt tcaccccgct
     2341 caccgaccac ccgccgctgg tcgagaccgt gcccggcgat atcgactacg agccgatcca
     2401 cacgatcttc aacgtcaagg tcaccgacaa gtacgccggc cagaagatca ccacgctcgg
     2461 cgcactgtcc gatgcgatcg acctcggcct ggtcgaggcg ccggtcgcga tcaaggtgtt
     2521 cgtcaactgg ccgatcgtcc gccccgggct ccggctcgag gtcggcaccg gcgccacggt
     2581 cccgccgact ccggcctacg cgcacggcta cgtcgtcgat ggcttcccgc tcggcggcat
     2641 gctcggccgg cagcccaacc cgcgcggcct gctgccgacc agccaggtca gcttcctgcg
     2701 cgaggcgggc acgccgacct tcgaccgggc ccatccgatc ttccaggcca ccatcccgac
     2761 gagccccccc ggtacgagcc cgaactacac gccggtcagc ctggtggtct atgtggacct
     2821 cgtgcagccc ctgaagccga cggacatcca caaggacagc gagctgttca cccgctcggc
     2881 gagtggcgac atcgtcagcg ccgacctgtc caaggtggca cagttcaccg tgacgacaaa
     2941 gctcaccgat ctgcagatcc agttcgcgga gggcgcgcca tgaccgcgcg
//
LOCUS       DASVOR010002328         2483 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_376147,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002328 DASVOR010000000
VERSION     DASVOR010002328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376147"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1678)
                     /locus_tag="VF516_42905"
     CDS             complement(<1..1678)
                     /locus_tag="VF516_42905"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114578.1"
                     /translation="MGEVVLAHDDRLDRLVAIKRLHDDSATPERRERFRREARIAARL
                     DHPAIVRIHDVLHEAGHDYLIMEYVEGQTLRARCAAGPMAVSEALGIAHQIALGMAAA
                     HDLGVIHRDLKAENVLITRAGRAKLTDFGIAKLDGEDTVTARGAVVGTFRAMSPEQAL
                     GRPIDHRSDLFSFGSLLYEALAAESPFRAETPFLTVQRLVNDEPRPIAELVPAIPEGL
                     ASLVHQLLAKEPLLRPRDFHEVADALIELAGQVCDAPCRVGSSLDGLPRSGEDRRSTE
                     DSVSTTAAPPDAGEPPPPPAPERQRTAPRRHPWRIALLVAAVLGAVGLGYALLGAGRA
                     RRIGVDGTSPGAAASPPIVRVAVLAPVVSGADGRNIPLLVAAVRNAVETSVQARVGLD
                     LIPRGDIDSYVDGYRRDTRRPPTQDDKRDAVGAEEVLTTDLSCSPDSCQVTLKRDAST
                     PGGPRSVLFRIDADSISRPDETVGVQLSRLYPGHPVRDAAATGSIDPTDYERYIRLVH
                     DYWAGDGTRSTSTEEVLVELERMRARSPSSIDMLLFEAEVLRHSYGQTQDP"
     gene            complement(1751..>2483)
                     /locus_tag="VF516_42910"
     CDS             complement(1751..>2483)
                     /locus_tag="VF516_42910"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEX8114579.1"
                     /translation="DVRIIAATDARLEALIASGAFGAPLLHRLAGYEIHLPPLRERRD
                     DIARLLLHFLGLDLAETGDTELLDRTEPAPWLPLTLARRIMQHGWPGNVRQLRNVVRQ
                     IVIGSRGETEASLSPALLHQLGDGAPAPAPVVETKVPSGRSRAIESRKHRRPADITET
                     ELVDALCSHRWDIKSTAAALGISRTSLYKLIETTDRVRTAGHLGVDEIRACHRDLGGD
                     LDAMVERLRVSKHALRRRIQELGLA"
BASE COUNT          330 a          827 c          977 g          349 t
ORIGIN      
        1 cggggtcctg ggtctgcccg tagctgtgcc ggaggacctc ggcctcgaac aggagcatgt
       61 cgatggagct cggcgaccgc gcgcgcatgc gctccagctc gacgagcact tcctcggtgg
      121 aggtggagcg agtgccgtcc cccgcccagt agtcgtggac cagtcgaatg tagcgctcgt
      181 agtccgtcgg atcgatcgag ccggtcgcgg cggcgtcgcg cacgggatga cccggataga
      241 gtctggacag ctggacgccg accgtctcgt cggggcgact gatgctgtcg gcatcgatcc
      301 ggaacaacac cgaccgcggg ccaccgggcg tgctcgcgtc gcgcttgagg gtgacctggc
      361 agctgtcggg cgagcacgag aggtcggtcg tgaggacctc ttcggcgccc accgcgtcgc
      421 gcttgtcgtc ctgcgtcggc ggacggcggg tgtcccgcct gtagccgtcg acgtagctgt
      481 cgatgtcgcc ccggggaatg aggtcgaggc cgacccgcgc ctgcacgctg gtctcgacgg
      541 cgttgcgcac ggcggcgacg agcagcggga tgttgcgacc gtcggcgccc gagacaacgg
      601 gggccagcac ggcgacgcgg acgatcggcg ggctcgctgc cgcaccgggg ctggtcccgt
      661 cgacgccgat gcgccgggcc cggcccgcgc cgagcaacgc gtagccgagg cccacggcgc
      721 cgaggacagc ggcgacgagc aaggcaatcc gccacgggtg gcggcgcgga gccgtgcgct
      781 gtctctccgg agccggggga ggcggcggct cgccggcgtc gggtggcgcg gcagtcgtcg
      841 aaacgctgtc ctcggtggac cgcctgtcct cgccggaccg cggcagcccg tccagcgacg
      901 agccgacgcg gcacggtgcg tcgcacacct ggccggccag ctcgatcagg gcgtcggcga
      961 cctcgtggaa gtcgcgcggt cgcagcagcg gctccttggc caggagctgg tggacgaggc
     1021 tcgcgaggcc ctccgggatc gccgggacga gctcggcgat cggtcgcggc tcgtcgttga
     1081 ccagccgctg gacggtcagg aacggggtct cggcgcggaa cggcgactct gcggcgagcg
     1141 cttcgtacag gaggctgccg aacgagaaca ggtcggagcg gtggtcgatc ggacggccca
     1201 gcgcctgctc gggcgacatg gcgcgaaacg tgccgaccac ggcgcctctc gcggtgacgg
     1261 tgtcctcgcc gtcgagcttg gcgatgccga agtcggtgag cttggcgcgg cccgcccgcg
     1321 tgatcagcac gttctcggcc ttgaggtcgc gatggatgac gccgaggtcg tgcgcggcgg
     1381 ccatgccgag cgcgatctga tgggcgatgc cgagcgcctc ggacaccgcc atcggccccg
     1441 cggcgcagcg cgcccggagg gtctggccct cgacgtactc catgatcagg tagtcgtggc
     1501 cggcctcgtg gagcacgtcg tggatccgca cgatcgccgg gtggtcgagc cgggcagcga
     1561 tccgcgcctc gcggcggaac cgctcgcggc gctccggagt cgccgagtcg tcgtgcaggc
     1621 gcttgatcgc gaccaagcga tcgaggcggt cgtcgtgggc gagcacgacc tcgcccatgc
     1681 cgcccgcgcc cagtctccgg atgacgcgat acggtccgat tcgctcggga tccgccatcg
     1741 aggagcgaga ttatgccagg ccgagctcct ggattcgacg ccgcagggcg tgcttggaca
     1801 cccggaggcg ctcgaccatg gcgtcgaggt cgcccccgag atcgcgatgg caggcgcgga
     1861 tctcgtcgac cccgaggtgg ccggcggtgc ggacgcggtc ggtggtctcg atcagcttgt
     1921 agagcgacgt gcgcgagatg ccgagcgccg cggcggtgga cttgatgtcc cagcggtggc
     1981 tgcacagcgc atcgaccagc tcggtctcgg tgatgtccgc cggccggcgg tgcttgcgcg
     2041 attcgatcgc gcgcgagcga ccgctgggga cctttgtctc cacgacaggc gcgggggcgg
     2101 gcgcgccatc gccgagctgg tgcagcagcg cgggtgacag gctggcctcg gtctcgccgc
     2161 ggctgccgat cacgatctgc cgcacgacgt tgcggagctg gcggacgttg cccggccagc
     2221 cgtgctgcat gatccggcgg gcgagggtga gcggcagcca cggcgccggc tcggtgcgat
     2281 cgagcagctc ggtgtcgccg gtctcggcga ggtcgaggcc gaggaagtgc agcagcaggc
     2341 gcgcgatgtc gtcgcggcgc tcgcgcagcg gcgggaggtg gatctcgtag ccggcgagcc
     2401 ggtgcaggag cggcgcgccg aacgccccgg acgcgatcag cgcctcgagg cgggcgtcgg
     2461 tggcggcgat gatgcggacg tcg
//
LOCUS       DASVOR010002329         5326 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_26932,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002329 DASVOR010000000
VERSION     DASVOR010002329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26932"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="VF516_42915"
     CDS             <1..854
                     /locus_tag="VF516_42915"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HEX8114580.1"
                     /translation="DDVLPNDKQPRIGPKEDSELQRQIEANEGIFEPLLLEPHPALGD
                     KFRIIDGDRRWSNSKVLVDQLRKPQYRQLPAEITDHTLDDEERLRVWIYIHRQRKEWD
                     AKEKEMVACRLVELVGRASAANILGITVREVDKLVDVYRLAERLKNIRDPSASITWAR
                     EINSINKNLLPPSVLDTIVDKINHKLITNSKDVRRLRAILKDPVSKDCFLSGGTSIND
                     ALEKLGEPAPQPRTNGLAGDLGAIVASIRKHPWTALAELRGNEDALRTIEEAENLLKE
                     LKKALQS"
     gene            complement(1140..1412)
                     /locus_tag="VF516_42920"
     CDS             complement(1140..1412)
                     /locus_tag="VF516_42920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114581.1"
                     /translation="MKNEVDGAVEDERGLGSAEAAGSAKSSAKGLLEVRNGVKVRESG
                     GVQDPRAHGSTQAAEHTGDEVTGSAMAGAAADEGLRGSAGAAGYLE"
     gene            1460..2146
                     /locus_tag="VF516_42925"
     CDS             1460..2146
                     /locus_tag="VF516_42925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114582.1"
                     /translation="MAQYLVSDEAARLHLLRERKYHALDGGARARFYSESRTSIAAYH
                     RGALSRDAIESKIVTMRYEAKFSNPLPRAELLSNADILERYLYYQGHRDLRLAPPPAA
                     DLVRGDVEIRVRPSLFAIEDDSARRLIFLELREKPHPAAMRVIAELAFEAFRPVLHYL
                     PPQAVQVIDVRRGMITELQQAGPSISRELAAACKAISAVWPHLAPLKGSPAVARAMER
                     QMSIAWDAER"
     gene            complement(2165..2713)
                     /locus_tag="VF516_42930"
     CDS             complement(2165..2713)
                     /locus_tag="VF516_42930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114583.1"
                     /translation="MKLTPLFLLVLVACGSDPVSFSSPVGINLKAKSGDVSSSAVSDD
                     KAITTESGNPYGAFVSDAQRKLGKDPGRIAIDKLTLTLGAQSTNVTALEQVYTGDVDV
                     AFITNDTNNTYDVGHVVSPTGVGPVDMRVTFASEDMTTADFTKLLTGSFKVVIRGPAA
                     TTFATSSAEANVQLMFTFAAFE"
     gene            complement(2817..5093)
                     /locus_tag="VF516_42935"
     CDS             complement(2817..5093)
                     /locus_tag="VF516_42935"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEX8114584.1"
                     /translation="MTSSNRVVATIAGLAIACGLTHHSAAQPAPGTSNDRAGSAPAPG
                     GDGPPVTTPDAPPGPLVDSSSGTSVTAPDDAAAAPASELPDPPSEADAEVIEINDRAP
                     AESASSVHLSRDDLQVRSRTQVSDLLRQVPGLMVSQHAGGGKSDQYFIRGFDADHGTD
                     IAIYADGVPVNLPSHAHGQGYADTHFLIPETISTVDVHKGPYSARFGDFYTAGAMELQ
                     TIDHVDGPTLWIAGGVPLAGPKSFEQYNRRVVGMASPEVRDSDTDRSLIAAQIADTDG
                     PFENAQRFRQGNALVKWQGQVGRGDLHLETSWYAGSWNASGQIPESAVAQGLIGRFGS
                     LDPSEGGDTSRTSLQLGYRVRDDHGGLWRASLFGLEYRLRLFSDFTLFARDPAHGDQI
                     EQDDARTVWGLDAAYERHFSLAELDTYLTAGVQLRDDNAETGLWHTEKRMRLPACFDL
                     GASPCTNDVHRIRDVSGYVEATIHLPPHIHILPGLRFEQVGWDVDDLAPATRSDPTRT
                     TGGSASRAIVLPKLSIEAEVTPKLDLFANAGSGFHSNDARGSVASHGDGALARGLGAE
                     AGLRTTVVPHARLAADVWYLHLDSELVWNGDEGGTSPSGPTRRYGVDLEAEVSPLPWL
                     RFDGNLSLAHAAFVHNAGNGSALALAPRIMGQGGVTLLHGPAFVSLRARGIGDRPGND
                     RGTLTAQGYLIFDLIAGRTFGKLDLNLTVNNLLNSDWREAQFADTSAIVPGGPAVEQM
                     HFTPGIPLTATATAAYRF"
BASE COUNT          804 a         1867 c         1836 g          819 t
ORIGIN      
        1 tcgacgatgt cctgcccaac gacaagcagc cgcggatcgg ccccaaggag gattccgagc
       61 tgcagcgcca gatcgaggcc aacgagggca tcttcgagcc gctgctgctc gagccccacc
      121 cggcgctggg cgacaagttc cggatcatcg acggcgaccg ccggtggtcc aactccaagg
      181 tcctggtcga tcagctcaga aagccgcagt accgccagct gcccgccgag atcaccgacc
      241 acacgctcga cgacgaggag cggctgcggg tctggatcta catccaccgc cagcgcaagg
      301 agtgggacgc caaggagaag gagatggtcg cgtgccggct ggtcgagctg gtcggccgcg
      361 ccagcgccgc caacatcctc ggcatcaccg tgcgcgaggt cgacaagctg gtcgacgtct
      421 accggctcgc cgagcggctc aagaacatcc gcgacccgag cgcgtcgatc acctgggcgc
      481 gcgagatcaa cagcatcaac aagaacctgc tgccgcccag cgtgctcgac accatcgtgg
      541 acaagatcaa ccacaagctg atcaccaact ccaaggacgt ccgccggctg cgcgccatcc
      601 tcaaggaccc cgtgtccaag gactgcttcc tgtccggcgg cacgtcgatc aacgacgcgc
      661 tcgagaagct cggcgagccc gcgccgcaac cgcggaccaa cggcctggcc ggcgacctcg
      721 gcgcgatcgt cgcgtcgatc cgcaagcacc cgtggaccgc gctcgccgag ctgcgcggca
      781 acgaggacgc gctgcgcacc atcgaagaag ccgagaacct gctcaaggag ctgaaaaaag
      841 cactccagag ctgatcccca ctccgccacg tacgcctttc ctttccaggc cgtacttccc
      901 gcagcctccg cgctgccgtg gactcgttcg tcctggaatc gccgacccag ctcgaccacc
      961 ccacctttca ctcggaacac cgcctgtttc gcagcctccg cgctgccgtg gatggcttcg
     1021 tcctcggcgc gcccggcaac cactccgcgt ttcaccttcc actccccact tccagcagcc
     1081 tccgcgctgc catgggctcc ctcatctttg acactgcctt gccgctcacc ttcactccct
     1141 cattccagat atccagcagc ccccgcgctg ccacggagac cctcgtccgc ggcggcgccg
     1201 gccatcgcac tccccgtcac ctcatcgccc gtgtgttcag cagcctgtgt gctgccgtgg
     1261 gcgcgtgggt cttgaacacc gccgctctcc cttaccttca ccccgttccg cacttctagc
     1321 agccctttcg cgctcgactt cgcacttcca gcagcctccg cgctgcccag gccccgctcg
     1381 tcctcgacgg cgccgtccac ctcgttcttc acctaccgcc agtggagtat ctcagatgat
     1441 caggctcacg gttcgcgaca tggcgcagta cctcgtctcc gacgaggccg cgcgactgca
     1501 cctcctccgc gagcgcaagt accacgccct ggatggcggt gcgcgcgcgc ggttctactc
     1561 cgaatcacgg acctcgatcg ccgcctatca ccgcggcgcg ctgagccggg acgcgatcga
     1621 gtccaagatc gtcaccatgc gctacgaggc gaagttctcc aacccgctgc ccagggccga
     1681 gctgctcagc aacgccgaca tcctggagcg ctacctgtac taccagggcc accgcgacct
     1741 ccgcctggcg ccgccgccgg ccgccgacct ggtgcgcggc gacgtcgaga taagggttcg
     1801 cccgtcgctg ttcgcgatcg aggacgacag cgcgcgccgg ctgatcttcc tcgagctgcg
     1861 cgagaagccc cacccggcgg cgatgcgcgt catcgccgag ctcgcgttcg aggcgttccg
     1921 cccggtgctg cactacctgc cgccccaggc ggtccaggtg atcgacgtgc gccgcggcat
     1981 gatcaccgag ctccagcagg ccggcccctc gatcagccgc gagctcgccg cggcgtgcaa
     2041 ggcgatcagc gcggtgtggc cccacctcgc gccgttaaaa gggtcgccgg cggtcgcccg
     2101 ggccatggag cgccagatgt cgatcgcctg ggacgccgag cgctgaccgc tcgggcccgc
     2161 cgcgctactc gaacgcggcg aacgtgaaca tcagctggac gttggcctcg gcgctgctgg
     2221 tggcaaacgt cgttgcggcc ggcccgcgga tcaccacctt gaagctcccg gtcaggagct
     2281 ttgtgaagtc ggccgtggtc atgtcctccg atgcgaacgt gacgcgcatg tcgaccgggc
     2341 ccacgccggt cgggctcacg acgtggccga cgtcgtaggt gttgttcgtg tcgttggtga
     2401 tgaaggcgac atcgacgtcg ccggtgtaaa cctgctcgag cgcggtgacg ttggtcgatt
     2461 gtgcgccgag cgtcaacgtg agcttgtcga tcgcgatgcg gccgggatcc ttgccgagct
     2521 tgcgctgggc atcactgacg aatgcgccgt agggattgcc gctctcggtc gtgatcgcct
     2581 tgtcatcgct gaccgcactg ctcgagacgt ctcccgactt tgccttgagg ttgatcccga
     2641 cgggactcga gaacgagacc ggatcggaac cgcacgcgac aaggacgaga agaaaaagtg
     2701 gcgtgagctt catgggatct cccgaggcga cggcacacct cgctgtgcat cagtcgtccc
     2761 ggcccgccag aggttcattg tgtgcacggc cggtgcagct ccaatgtagg cctgtgctag
     2821 aaccggtacg cggcggtcgc ggtcgcggtc agcgggatgc cgggcgtgaa gtgcatctgc
     2881 tcgaccgccg ggccgccggg gacgatcgcg gaggtatcgg cgaactgggc ctcgcgccag
     2941 tcgctgttga gcaggttgtt gacggtcagg ttgagatcga gcttgccgaa cgtgcggccg
     3001 gcgatcaggt cgaagatcag gtagccctgc gcggtcagcg tgccgcggtc gttgcccggg
     3061 cggtcgccga tgccgcgcgc gcgcagcgag acgaacgccg ggccgtgcag cagcgtgacg
     3121 ccgccctggc ccatgatccg gggcgcgagc gccagggcgc tgccgttgcc ggcgttgtgg
     3181 acgaacgccg cgtgggcgag gctgaggttg ccgtcgaacc gcagccacgg cagcgggctg
     3241 acctcggcct cgaggtcgac gccgtagcgc cgggtcggcc ccgacggcga cgtgccgccc
     3301 tcgtcgccgt tccacaccag ctcggagtcg aggtgcaggt accagacgtc ggcggcgagc
     3361 cgggcgtgcg ggacgacggt ggtgcgcagc cccgcctcgg cgcccaggcc gcgggcgagc
     3421 gcgccatcgc cgtggctggc gacgctgccg cgcgcgtcgt tggagtggaa cccgctgccg
     3481 gcgttggcga acaggtcgag cttgggggtg acctcggcct cgatcgacag cttgggcagg
     3541 acgatcgcgc ggctggcgct gccgccggtg gtccgcgtcg gatcgctgcg ggtcgccggg
     3601 gcgagatcgt cgacgtccca gccgacctgc tcgaagcgca ggccgggcaa gatgtggatg
     3661 tgcggcggca ggtggatcgt cgcctcgacg tagccggaca cgtcgcggat ccggtggacg
     3721 tcgttggtgc atgggctggc gcccagatcg aagcacgcgg ggagccgcat ccgcttctcg
     3781 gtgtgccaca gcccggtctc ggcgttgtcg tcgcgcagct gcacgccggc ggtcaggtag
     3841 gtgtcgagtt cggcgagcga gaagtggcgc tcgtaggcgg cgtcgaggcc ccacaccgtg
     3901 cgcgcgtcgt cctgctcgat ctgatcgccg tgcgccgggt cgcgcgcgaa cagcgtgaag
     3961 tcggagaaca ggcgcagccg gtactcgagg ccgaacagcg acgcgcgcca cagcccgccg
     4021 tgatcgtcgc ggacgcggta gccgagctgg aggctggtgc gcgaggtatc gccgccctcg
     4081 ctgggatcga gcgagccgaa ccggccgatc aggccctggg cgaccgcgga ctcggggatc
     4141 tggcccgagg cgttccagct cccggcgtac cagctggtct cgaggtggag gtcgccgcgg
     4201 ccgacctggc cctgccactt gaccagcgcg ttgccctggc ggaaccgctg ggcgttctcg
     4261 aacggcccgt cggtgtcggc gatctgcgcc gcgatcagcg atcgatcggt gtcgctgtcg
     4321 cgaacctccg ggctcgccat gccgacgacc cggcggttgt actgctcgaa gctcttcggg
     4381 ccggcgagcg gcacgccacc ggcgatccac agcgtcggcc cgtcgacgtg gtcgatggtc
     4441 tggagctcca tcgcgccggc ggtgtagaag tcgccgaacc gcgccgagta cgggcccttg
     4501 tggacgtcga cggtcgagat cgtctcgggg atcaggaagt gggtgtcggc gtagccctgg
     4561 ccgtgggcgt ggctcggcag gttgaccgga accccgtcgg cgtagatcgc gatgtcggtg
     4621 ccgtggtcgg cgtcgaaccc gcggatgaag tactgatcgg acttcccgcc gccggcgtgc
     4681 tgcgacacca tcaggccggg gacctggcgc aggaggtccg agacctgggt ccgcgagcgg
     4741 acctgcaggt catcgcgcga caggtggacc gaggacgccg actcggccgg cgcgcggtcg
     4801 ttgatctcga tgacctcggc gtcggcctcg ctcggcggat cgggcagctc ggacgccgga
     4861 gcggccgccg cgtcatcggg cgcggtcacc gaggttccgg acgaggaatc aaccagtgga
     4921 ccgggcggcg catccggtgt cgtgaccggc ggcccatccc cgcccggagc aggcgcgctg
     4981 ccggcccggt cgttcgacgt ccccggcgcg ggttgtgcag cgctgtgatg cgtcaggccg
     5041 caggcgatcg ccaggccggc gatcgtcgcg acgacgcggt tcgaagacgt catggcagtt
     5101 ccattcgcgt agctgtccat cgcgcgcacg acggcgcgcg accgcaaccc accggtcggt
     5161 gcgcgagcac gcgcgccggc aggtcgctcg ttcagagaat cggactacgc gaaactcgga
     5221 gggccgcgcg cggtggcggt tggcggcgga cgtacggaga tcgccgagcc ggtgcggacg
     5281 gcgagcagga ccggcctgcg gtcgaccggc agcgggtgca ggatcg
//
LOCUS       DASVOR010002330         7664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_493275,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002330 DASVOR010000000
VERSION     DASVOR010002330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7664
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493275"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF516_42940"
     CDS             <1..244
                     /locus_tag="VF516_42940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114585.1"
                     /translation="FMAGWLLASAIAVVVGGRFFGHYFPQLTAPLAVLAAPAMVRLWQ
                     RRRAVIRSCGSWPAGCWSAGSRWSSAAGSSATTSTS"
     gene            157..858
                     /locus_tag="VF516_42945"
     CDS             157..858
                     /locus_tag="VF516_42945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114586.1"
                     /translation="MRFLAGWLLVSGIAVVVGGRVFGHYFHQLTAPLAVLAAPATVRL
                     WQRRRAVVCLAIAVPAAVYLVLGALHDRVMRWYGEPDPDYPSVVAWLDARGAGALCVW
                     GNSPVLYFEANRPLGCRFVFSNYLTGASPAPATQIDAAVDSSRYIVREAWPMLEADLD
                     QRRPRFIVDGSPGDVALYGKYPPSKFPPLARVLGCAYQPVADVAGMRIYERREAPCAE
                     PRAVRADDPGAGGVQ"
     gene            883..2361
                     /locus_tag="VF516_42950"
     CDS             883..2361
                     /locus_tag="VF516_42950"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8114587.1"
                     /translation="MTDDDRKLGRGTAWVGIASGLSGVLDLITTAACLWLWLSTEDLG
                     TATLAGALLPVIERFASAGMPAAMVRWGDGDRRALSTMLWLGVASSCAVLAALVGVGP
                     WIGRAFGAPIIGSLLIGYGVKLVLQNAHVVPEALLRRELAFDRLTRVRIAASTGDAIA
                     KLVAAYLGAHGYPALRIWCFAIGPLANGVVTSIGIQLCRPWWPALAFDRAVAAGALRY
                     GAQVAGGELLYFVYTSADYLVIGAAWGKAAVGAYRLAYELVLDVVRLISLVTAEVAFP
                     AFARPADDARAGALLIRFTRQNAIALVPVVAFLAVEAGDLLAVLYPPLGPAATTAARI
                     LCVVGGLRMLSFVLPAMLAGIGHAGDALLYHVLACVVLPGAFALAAWLAPGAGYVAVA
                     WAWAAGYPVVFAVLLERALVRGRVRLASYLRSVAGVLVCGAGAAIGAIAVHAVLPGPP
                     IGRLVAVAAVIVAIYTGLIARIERITPRSILRTVRGMAEPGA"
     gene            2358..3965
                     /locus_tag="VF516_42955"
     CDS             2358..3965
                     /locus_tag="VF516_42955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114588.1"
                     /translation="MISRRLAALSDRALWLAIGAALFALSAWPLVLVELPPFQDLPNH
                     VASAYIARHLDLYPDYVFNGLWKSNSALELWLHPFADDHLLSAARAFVAIVLAVNAFG
                     LPWFVLQLAGRRAMVNASLLIWPLVHGFCLAMGMCNFALAVPLSLVVLVLLGRQRTAP
                     SAGLSIAIVALSVAVWLAHPFPLIVVGGVCLIELIARRDRWWRAVVTLLPLAPIAVLI
                     AGTALHHLIKAEGAPVTASRGFEFLTPWELPLHFWLDVSGALTRWSAATVVPAIALPI
                     LAWRHRREPAPLTGWTTVALLVLYLGLPLMISNWWYLNTRVAPFVWIALAVRVPPALP
                     RKLVALLIASALAFSAALGVDYLRLDRDRAELTAGIDAVPARATLLPLLFQHRRTSDF
                     TASLTHAWGYYVLARQTSAPLVFAVERSYAITYRRFPPPALIPPALDRFAELHASPAQ
                     LCDGVHPVATNLADCTAIWQLLWSQFWAEAEPRFSHVLTWAMPPEARAMMPASYRVVF
                     AAGDLAIWARGGSAVSSMQAPIAPAAR"
     gene            4119..5714
                     /locus_tag="VF516_42960"
     CDS             4119..5714
                     /locus_tag="VF516_42960"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEX8114589.1"
                     /translation="MVIAVVGLAVALVGTLVGLAACLARLAKLRRRFSAIVDVDAEVA
                     RLRASALQELAQLKAVAAEDAARLRAEGQRYRREAQEGLAQAQAEGTRLRAAVADEAE
                     SIHRAIQREREQQARVASDTARVRADFERLSDELRKVEGSLEDISFGLYKPQYQFDTP
                     EQFKAEMDRVYDRQKDMVRAGKAASFAVSWTVGNSRKEGERMQKQYSKLLLRAFNGEC
                     DAAIAKVNWNNATKMEERIRKAFDAINQLGEVMTVSLSPGYRDLKLAELRLEHELAEK
                     KREIAEEQRRIRDQMRDEEKALREAEKAQADAEEEEARFQKALEKAKVELSRARGEEH
                     ARLNDKILELQGQLAEARAKSVRARSLAEMTKAGYVYIISNIGSFGEQVFKIGMTRRL
                     DPMDRVRELAAASVPFPFDVHAMVYSEDAPALEAAFHQRFKDRSVNLVNLRKEFFHVD
                     LAELEAFAKQRGLKIAFTKVAEAREYRESAAMRLARGSGPTTAPSLQDQVAMGSALAK
                     LSKSPSEDRAGGAAGLPRPARPS"
     gene            complement(6067..6828)
                     /locus_tag="VF516_42965"
     CDS             complement(6067..6828)
                     /locus_tag="VF516_42965"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8114590.1"
                     /translation="MATEKREVQRRRRRSEPEQLALWKKRGGKRRGAGRPPNGPRSGS
                     PHKERPYLHARYPVHVTLRVVGEVGNLRRRCVYQAVREATLTTARRENFRIVHLSIQR
                     THVHLLVEADDRSALASGMQGFQISAAKHLNAAISKGKPGPRRRGAVFPDRYHAEIIT
                     SPRQARNALSYLMNNFRRHQEDLRAPMSGWTIDWFSSAVMFPGWAEYGDEPFLWRGPA
                     TYDPLLVYQPRTWLLREGWKKAGTISCHEVPAARR"
BASE COUNT         1027 a         2621 c         2741 g         1275 t
ORIGIN      
        1 gttcatggcc gggtggctgc tggccagcgc gatcgcggtg gtcgtcggcg gccggttctt
       61 cggccactac tttccccagc tgaccgcgcc gctcgcggtg ctcgcggcgc cggcgatggt
      121 ccggctgtgg cagcgccggc gcgccgtgat ccgttcgtgc ggttcctggc cggctggctg
      181 ctggtcagcg ggatcgcggt ggtcgtcggc ggccgggtct tcggccacta cttccaccag
      241 ctgaccgcgc cgctcgcggt gctcgccgcg ccggcgacgg tccggctgtg gcagcgccgg
      301 cgcgccgtgg tctgcctcgc gatcgcggtc ccggccgcgg tctacctcgt gctcggcgcg
      361 ctccacgacc gggtgatgcg ctggtacggc gagccggatc cggactaccc gagcgtggtc
      421 gcgtggctcg acgcgcgcgg cgccggcgcg ctgtgcgtgt ggggcaactc gccggtcctg
      481 tacttcgagg ccaaccgccc gctcggctgc cggttcgtgt tctccaacta cctgaccggc
      541 gcgtcgccgg cgcccgcgac ccagatcgac gcggcggtcg actcgtcgcg ctacatcgtg
      601 cgcgaggcgt ggccgatgct cgaggccgac ctcgaccagc gccggccgcg gttcatcgtc
      661 gacggctcgc ccggcgacgt cgcgctctac ggcaagtacc cgccgtcgaa gttcccgccc
      721 ctggcgcgcg tgctcggctg cgcgtatcag ccggtcgccg acgtcgcggg catgcggatc
      781 tacgagcggc gggaggcgcc gtgcgccgag ccccgcgcgg tgcgcgccga cgacccggga
      841 gcgggcgggg tacagtgatg ccgcgcgctc gcgctgccgg ccatgacaga cgacgaccgc
      901 aagctcgggc gaggcacggc gtgggtcggg atcgcgagcg gcctgtccgg cgtcctcgac
      961 ctgatcacga ccgcggcctg cctgtggctg tggctgtcga ccgaggacct cgggaccgcg
     1021 acgctggcgg gcgcgctgct gccggtgatc gagcggttcg ccagcgccgg catgccggcg
     1081 gcgatggtgc ggtggggcga cggcgaccgc cgcgcgctgt cgaccatgct gtggctgggc
     1141 gtcgcgagct cgtgcgcggt gctcgcggcg ctggtcggcg tcgggccgtg gatcggccgg
     1201 gcgttcggcg cgccgatcat cggcagcctg ctgatcggct acggcgtcaa gctggtgctg
     1261 cagaacgcgc acgtcgtgcc cgaggcgctg ctgcgccgcg agctcgcgtt cgatcgcctg
     1321 acccgcgtgc ggatcgcggc gtcgaccggc gatgcgatcg ccaagctggt cgccgcgtac
     1381 ctcggggcgc acggctaccc cgcgctgcgg atctggtgct tcgcgatcgg gccgctggcc
     1441 aacggcgtgg tgacgtcgat cgggatccag ctgtgccggc cgtggtggcc ggcgctcgcg
     1501 ttcgatcgcg cggtcgcggc cggcgcgctg cgctacggcg cgcaggtcgc cgggggcgag
     1561 ctcctgtact tcgtctacac cagcgccgac tacctggtga tcggtgcggc gtggggcaag
     1621 gccgcggtcg gcgcctaccg gctggcgtac gagctggtgc tcgacgtcgt gcggctgatc
     1681 tcgctggtca ccgccgaggt cgcgttcccc gcgttcgcgc ggccggcgga cgacgcgcgc
     1741 gccggcgcgc tgctgatccg gttcacccgg cagaacgcga tcgcgctggt tccggtggtc
     1801 gcgttcctcg cggtcgaggc cggcgacctg ctcgcggtgc tgtatccgcc gctcgggccg
     1861 gcggcgacca ccgcggcgcg catcctgtgc gtggtcggcg ggctgcgcat gctcagcttc
     1921 gtcctgccgg cgatgctggc cggcatcggc cacgccggcg acgcgctgct ctatcacgtg
     1981 ctggcgtgcg tcgtcctgcc gggggcgttc gcgctcgcgg cgtggcttgc gcccggcgcc
     2041 ggctacgtcg cggtcgcgtg ggcgtgggcg gcgggctatc cggtcgtgtt cgccgtgctg
     2101 ctcgagcgcg cgctggtgcg cggccgggta cggctggcga gctacctgcg cagcgtcgcc
     2161 ggcgtgctgg tgtgcggcgc gggggcggcg atcggggcga tcgccgtgca cgcggtgctg
     2221 cccgggccgc cgatcgggcg cctggtcgcg gtcgcggcgg tgatcgtcgc gatctacacc
     2281 ggcctcatcg cgcggatcga gcggatcacg ccgcgctcga tcctgcgcac ggtgcgcggc
     2341 atggctgagc ccggagcatg atctcgcgaa ggctggcggc gctgtcggac cgcgcgttgt
     2401 ggctggcgat cggcgccgcg ctgttcgcgc tgtcggcgtg gccgctcgtg ctggtcgagc
     2461 tgccgccgtt ccaggacctg cccaaccacg tcgcctcggc gtacatcgcc cgccacctcg
     2521 acctctaccc cgactacgtg ttcaacggcc tgtggaagtc gaactcggcg ctggagctgt
     2581 ggctgcaccc gttcgccgac gatcacctgc tgtccgccgc ccgggcgttc gtggcgatcg
     2641 tgctcgcggt caacgcgttc gggctgccgt ggttcgtgct ccagctcgcc ggccggcggg
     2701 ccatggtgaa cgccagcctg ctgatctggc cgctggtcca cggcttctgc ctcgcgatgg
     2761 gcatgtgcaa cttcgcgctc gcggtgccgc tgtcgctcgt cgtgctggtc ctgctcggcc
     2821 gccagcgcac ggcgccgagc gcggggctct cgatcgcgat cgtggcgctg tcggtcgcgg
     2881 tgtggctcgc ccatccattc ccgctgatcg tcgtcggcgg ggtctgcctg atcgagctca
     2941 tcgcccggcg cgatcgctgg tggcgcgcgg tcgtgaccct cttgccgctc gcgccgatcg
     3001 cggtgctgat cgcgggcacc gcgctgcacc acctgatcaa ggccgagggc gccccggtca
     3061 ccgcctcgcg cggcttcgag ttcctgaccc cgtgggagct gccgctccac ttctggctcg
     3121 atgtctcggg cgcgctcacc cggtggagcg ccgccaccgt ggtcccggcg atcgcgctgc
     3181 cgatcctcgc gtggcgccac cggcgcgagc ccgcgccgct gaccggctgg acgaccgtcg
     3241 cgctgctcgt cctctacctc ggtctgccgc tgatgatcag caactggtgg tacctcaaca
     3301 cccgggtggc gccgttcgtg tggatcgcgc tcgcggtgcg cgtgccgccg gcgctgccgc
     3361 gcaagctggt cgcgctcttg atcgccagcg cgctcgcgtt ctcggccgcg ctcggcgtcg
     3421 actacctgcg gctcgatcgc gaccgcgccg agctcaccgc cgggatcgac gcggtgcccg
     3481 cgcgcgcgac gctgctaccg ctgctgttcc agcaccgcag gaccagcgac ttcaccgcca
     3541 gcctgaccca cgcctggggc tactacgtgc tcgccaggca gaccagcgcg ccgctcgtgt
     3601 tcgcggtcga gcgatcctac gcgatcacct accgccggtt cccgccgccg gcgctgatcc
     3661 cgcccgcgct cgaccgcttc gccgagctgc acgcgagccc ggcgcagctg tgcgacggcg
     3721 tgcaccccgt cgcgaccaac ctcgccgact gcacggcgat ctggcagctc ctgtggtccc
     3781 agttctgggc cgaggccgag ccgcggttca gccacgtgct gacctgggcg atgccgcccg
     3841 aggcgcgcgc gatgatgccg gcgagctacc gcgtggtgtt cgccgccggc gatctcgcga
     3901 tctgggcacg tggcggatcc gcggtgtcgt cgatgcaggc gcccatcgcg cccgcggcgc
     3961 ggtgagctgt cgcgcgcgcg cgtgacggta gtatgttttt gacctacatc agttgaggcg
     4021 taccggcgcg gcgtgaaacg acgctccggg taacccgcag ctacccgttt gccatcgaca
     4081 acctcgctac ggtgggcatt caaccattgg aggtgtcgat ggtgatcgcg gtcgtcggat
     4141 tggcagttgc gctggtgggc acgctggtcg gcctcgctgc ctgcttggca cggcttgcaa
     4201 agctgcggag gcgcttctcg gcgatcgtgg atgtggacgc cgaggtcgcc aggcttcggg
     4261 cgtccgcgct gcaagaactg gcgcaactga aagccgtcgc ggcggaggac gccgccaggc
     4321 tgcgggcaga aggccagcgc taccggcgcg aggcacagga aggcttggcc caagcgcaag
     4381 cggaaggcac tcgtctgcgg gctgcggtcg ccgacgaagc cgaatcgatc catcgagcca
     4441 tccagcgcga gcgcgaacag caggcgaggg ttgcgagcga taccgcgcgg gttcgagccg
     4501 atttcgagcg gctcagcgat gagttgcgca aggtcgaagg aagcctcgaa gacatctcgt
     4561 tcggtctgta caagccgcag taccagttcg acaccccgga gcagttcaag gccgaaatgg
     4621 accgtgtcta cgaccgccag aaggacatgg tgcgcgcggg caaggcggcc agcttcgcgg
     4681 tgtcctggac cgtcgggaat agccggaagg agggcgagcg catgcagaag cagtacagca
     4741 agctcttgct gcgtgcgttc aacggcgagt gtgatgcggc cattgcaaag gtcaactgga
     4801 acaacgcgac caagatggag gagcggatcc ggaaggcatt cgacgcgatc aaccagctcg
     4861 gcgaggtcat gacggtgtcg ttgtcgcccg gctaccgcga cctgaagctg gccgagcttc
     4921 ggctcgagca tgagctcgcc gagaagaagc gcgagatcgc cgaggagcag cgccggatcc
     4981 gcgatcagat gagagacgaa gagaaggcgc tgcgcgaagc cgagaaggcg caagcagacg
     5041 ccgaagaaga ggaggcccgg ttccagaagg cgctggagaa ggccaaggtc gagctgtcga
     5101 gggcacgggg cgaagagcac gcgcgcctca acgacaagat cctggagctc caggggcagc
     5161 tcgccgaggc ccgtgccaag tccgttcgcg ccaggtctct cgccgagatg acgaaggccg
     5221 gctacgtgta catcatctcg aatatcggct cgttcggcga acaggtcttc aagatcggaa
     5281 tgacccgccg cctggatccg atggaccgcg tgcgtgagct cgccgctgcc tccgttccgt
     5341 tcccgttcga cgtccacgcg atggtctatt cggaggatgc tcctgccctg gaggcggcat
     5401 tccaccagcg gttcaaggat cgcagcgtga acctggtcaa tctgcgcaag gagttcttcc
     5461 acgtcgacct cgccgagctc gaggcgtttg cgaagcagcg cggactgaag atcgcgttca
     5521 cgaaggtcgc cgaggcgcgc gagtaccgcg aatcggccgc gatgcgcctc gctcggggta
     5581 gcggtccaac caccgccccg agtcttcagg atcaagtcgc catgggcagc gcgctggcca
     5641 agctgtccaa gtcgccgtcc gaagatcgtg cggggggcgc cgcgggtttg ccgagacccg
     5701 cgagaccatc ctgacgccgt accagctcgt acctgcagct cgctacttcg tgtcggagca
     5761 tcaggccgac gcctgccaga tacgaggcgg gcctccccgc tgacccagac gcggtcgtcg
     5821 cagcgcggtg atcgcaaccg gcggcgcgtt tgggtgcccg acctgggcat gacgcgcagc
     5881 cgttcgaact gtggtcgcac gtcccctcca ttcggcgagg aattcgtgac cggcaatgct
     5941 cgctcgaaga cctttgcgat acgcgtttgg atcaccgcct gacgcgcctc gcgcccaagt
     6001 gagctcggtc gggcgcgccg tccagcctgg aaagcgtgcg ccgaccggcg acagagtgca
     6061 cgggggtcac cgccgcgccg cggggacctc gtgacacgag atcgtcccgg ctttcttcca
     6121 gccctctctc agcaaccacg tcctgggttg atacaccaag agcggatcgt aggtcgcagg
     6181 gccgcgccac aggaagggtt catcgccata ctccgcccag cccggaaaca tcactgcgct
     6241 ggagaaccag tcgatcgtcc agccggacat cggagcccga agatcttcct ggtggcgacg
     6301 gaagttgttc atcaaataag atagagcatt ccgcgcctgg cgcggcgatg tgatgatctc
     6361 cgcgtggtaa cggtcaggga acaccgcacc tcgccggcgc ggcccaggct tccccttgct
     6421 gatcgccgcg ttgaggtgct tggcggcgga gatctggaag ccctgcatcc cggacgccag
     6481 tgcgctcctg tcatcggcct ccaccagcag gtgcacgtgc gtgcgctgga tgctgaggtg
     6541 gacgatgcga aagttctcgc gccgcgcggt ggtcagcgtg gcctcgcgga ccgcttgata
     6601 gacgcagcgg cggcgcaggt tgccgacttc accgacgacc cgcagcgtga cgtggacggg
     6661 gtaccgcgcg tggaggtacg gccgctcctt gtgcggcgat ccggatcgcg ggccgttcgg
     6721 cgggcgaccg gcgccgcgcc gcttgccgcc gcgcttcttc catagcgcca gctgctccgg
     6781 ctcggagcgc cgccgcctcc gttgcacctc gcgcttctcg gtggccatgc aagcttgata
     6841 attcctgagt tgcaatcagg caatcgattt cgccgttatt tattgggaca attgtctgct
     6901 atttctaaat tgtcgtgttc tggagacaag atcattgatc aatcattgac gatgggctgg
     6961 ttggcgactg ccgccacgaa cgcgaggcca cgactcagcg gactgcagcg acgaggttgt
     7021 gcaggacgcg tgaccacgct gtcacacctg atggacagcg cgccgccgct gacaccgttt
     7081 gccaggacgc cctgtccaca ggtctgacag cgctgacgcc cgcgcggggg ctcctgatcg
     7141 ggatttattc atattctttt cttcactcga gagagaaatc tctcttccag gagcctgcgc
     7201 gcgggcgtcg ggcctgtggg cggtggcgtg aggccggtga gctgtcgcgc cgaggtggac
     7261 cgatgccgct gcggcgcgca ggtcacgggc cgagccaccg tccacaggca gcactggatc
     7321 ttccgacctt gactggctgc tgctcgatcg agacctgcgc ggcttggcag gcgctcgcgg
     7381 aactcggcga gtcacggctc gagcagcggg cgcaacgggg tcttgcagct cgtcgcaagg
     7441 aggtacgacc gcagcagctt cctgctggag gactcgcgaa gtcacgcgtt ggaggttcat
     7501 cgtcctgccg gcgcccgcca gagccgagac tgcgggctga tgacgagctc ggccgcggca
     7561 tcaccgtgag ctgcaactcc ccgcgcgcgc gggtgtccga agggggcatg acgcgctgtc
     7621 attcgaagcc gtggccgcac ctcttcgccg gcgccgcggt cgcc
//
LOCUS       DASVOR010002331         1812 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_317827,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002331 DASVOR010000000
VERSION     DASVOR010002331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_317827"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VF516_42970"
     CDS             complement(<1..478)
                     /locus_tag="VF516_42970"
                     /inference="COORDINATES: protein motif:HMM:NF023169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEX8114591.1"
                     /translation="MSQPRSWIAAIAVAAAVVAPSAARADGKNEIRAVTFDGDGATTR
                     VHVQGAQTPTFTVYKLERPSRIVVDVPQARLGEALRGHESAALLAANTWAVSTIAAQQ
                     LDDGGLVVRVIITLARPGRYDVKTQGNELVVMVMARDPAPPTADPAALAAAQAEREQ"
     gene            complement(883..1695)
                     /locus_tag="VF516_42975"
     CDS             complement(883..1695)
                     /locus_tag="VF516_42975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114592.1"
                     /translation="MALRHPSARRWLVLVLALAGCGDDGVAPPPNNVPVPQPQVTPKP
                     GSPKGQLAVKPHVEDRVLCTPPDQPSDPKGGQCDPKAPSCGEHLYCLQLAQGFYCEPC
                     PERDGIRHAFRERDFDAEHNRDPFQRDSLQPLTGSGGPKPQPGDKCRADQLVASSYSY
                     ADLKLVGIVAQGTQRKVLMTVGKDGFIIKRGDCVGKEKAVVKDIGLGYIRFQGESDEH
                     EVELNPKQLQVNDPLPVPPRGPAAGSNSPGAPAEAPSGTAPGANPPPAPKKP"
BASE COUNT          235 a          652 c          637 g          288 t
ORIGIN      
        1 cctgctcgcg ctcggcctgc gcggcggcca gcgccgccgg atcggcggtc ggcggcgccg
       61 gatcgcgcgc catcaccatg accaccagct cgttgccctg ggtcttgacg tcgtagcggc
      121 cggggcgcgc cagcgtgatg atcacgcgga cgaccaggcc gccgtcgtcg agctgctggg
      181 cggcgatcgt gctgaccgcc caggtgttcg cggcgagcag cgccgcgctc tcgtggccgc
      241 gcagcgcctc gccgagccgg gcctgcggca cgtcgaccac gatccggctc ggccgctcca
      301 gcttgtagac cgtgaacgtc ggggtctggg cgccctgcac gtggacgcgc gtcgtcgcgc
      361 cgtcgccgtc gaacgtgacc gcgcggattt cattcttacc gtcggcgcgc gccgcggacg
      421 gcgcgaccac cgccgctgcg accgcgatcg cggcgatcca ggacctgggt tggctcatga
      481 acacgctcct cgcgcgccga aatgttcgcc cgctcggcgg agcggcacaa attttcgccg
      541 gcggccgcga tttacataag cgcaacccgc ctcccgctcg ctcgccccgg cgccgcacgt
      601 ctgcctcagg cggatcgccc ggtggacgtc gacgtggccc tggaccttga cggccgtcgt
      661 tgaggttgac gtggccctac gacgtcgagc ccatcgtcga cctccacctt gaccatcgct
      721 cgcgatgccc cgacgagggt tcagagacca ccagagggtc tacgtacaag gtcgaggatg
      781 gagtctacgt ccaacgtcgc cgttcaggtc gacgtcttgc gatcacatca acgtcgacct
      841 caacgtcttc gcaaggacga catttccacc ccggcagcgc catcacggct tcttcggcgc
      901 aggcggcgga ttcgcgcccg gagcagtacc gctcggggcc tcggcaggcg caccggggga
      961 attcgagccg gcggcggggc cgcgcggcgg caccggcagc gggtcgttga cctgcagctg
     1021 cttggggttg agctccacct cgtgctcgtc ggactcgccc tggaacctga tgtagcccag
     1081 gccgatgtcc ttgaccacgg ccttctcctt gccgacgcag tcgccgcgct tgatgatgaa
     1141 gccgtccttg cccaccgtca tcagcacctt gcgctgggtg ccctgggcga cgatcccgac
     1201 gagcttgagg tcggcgtagc tgtagctcga cgcgaccagc tggtccgcgc ggcacttgtc
     1261 gccgggctgc ggcttcggac cgcccgaccc cgtgagcggc tgcagcgagt cgcgctggaa
     1321 cgggtcgcgg ttgtgctcgg catcgaagtc gcgctcgcgg aatgcgtggc ggatgccgtc
     1381 ccgctcggga cacggctcgc agtaaaaacc ctgggcgagc tgcaggcagt acaggtgctc
     1441 gccacagctc ggcgccttgg ggtcgcactg gccgcccttg gggtcgctcg gctggtcggg
     1501 aggcgtgcag agaacgcgat cctcgacgtg cggcttcacg gcgagctggc ccttcggcga
     1561 accgggcttt ggcgtgacct ggggctgcgg gaccggcacg ttgttcggcg gtggggcgac
     1621 gccgtcgtcg ccgcacccgg cgagcgcgag cacgagcacg agccaccgcc gcgcagaggg
     1681 atgtcgcagc gccatggcta caggcctccc ttcagccggt cgctcccggc ggccggccgc
     1741 tcggcgggca cggccccggg ggtggcaccg gcggcaccgg cggccttgcg atccttggcg
     1801 tcgcccttct cg
//
LOCUS       DASVOR010002332         6613 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_114159,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002332 DASVOR010000000
VERSION     DASVOR010002332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6613
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114159"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            305..1471
                     /locus_tag="VF516_42980"
     CDS             305..1471
                     /locus_tag="VF516_42980"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8114593.1"
                     /translation="MASAVAVLLLLKFVQDRWRDELIRNNLGTVIYTIVPFDGRDGAI
                     VPATGDPMPGLTVTVYGAKADDTHEPGDPIPDKLVRIVAMGGAGNPRVQRISAPGGTV
                     FLRLDGRGRPGEHCPPSWIRVQAFPGYRIDDIKRIELPVPTCQATLADTVKIDAGPFW
                     YGGPGEPPAKHYGEPDYTEPERSIDLPAFAMDRTEVSNAAFEPFARLEKLTGYPRPIY
                     SRDGVNIHDGEPSYPVSDIDAFEAAAYCRYMGKRLPSDAEWTKSARGGLTVNGKPNPA
                     PRRLYPWGNTWKPECVNQDYAVNKQAWTRPVDSLACGASPYGILQLVGNVQEWIARDG
                     QTDRDNPLHALRGGSTDDAPGDETTTTVFRNHRDPRVLNYSNGARCVVDLEGAP"
     gene            1468..2475
                     /locus_tag="VF516_42985"
     CDS             1468..2475
                     /locus_tag="VF516_42985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114594.1"
                     /translation="MILSDQPRTVTLVANPPPETDNGGLDQTFRVLSTKGVRDNEIRR
                     VASVSDIDIALSNVLTPAFKATVRLQIIGHAIPGSMCLGAKWTDTGIHDPAAFAFPFL
                     VLDTNPTALGLLAKFAGKLSEVMLVGCHVGSTTSFGHAINGRTLTYTLAELLRCKVCG
                     ADDDVAPDEYDDQGWYAPAPQHRRPKGWRWSESSPPLWIDLGTDPLPRNRKPAGRNGK
                     PLEIEIRTIRTAMLPLLASPQPVDPSLDLPSSLRVTCQHTTARPVSALPELSVDTDLG
                     PAQILCGGRYLTIADVCYVLERNPPLTAALTALPRTIDAALRAPTTAAPPGGRAVPAA
                     G"
     gene            complement(2530..3234)
                     /locus_tag="VF516_42990"
     CDS             complement(2530..3234)
                     /locus_tag="VF516_42990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114595.1"
                     /translation="MVPQVTIESVDAQNVSFWTRSDSKTGDPNERLRRLFTVAVKEVW
                     TAPIPGQPAPVEMKPGATGPLFLSKTGWQTTTPDGYHILGAGTDAVAFKLPACLAAAP
                     FQGDLTVIPSKLVVQGYPEGKSELVQIDLLGPDAAESQDLRWHGSEYLEIRLAEHASG
                     PSADPHDGTVKVWPTQILVSGSAKSAPAISGEVDLLDGNLAAHAQHIQFAQRGTPISL
                     VLTQNGFGAFHVVPGM"
     gene            complement(3316..4737)
                     /locus_tag="VF516_42995"
     CDS             complement(3316..4737)
                     /locus_tag="VF516_42995"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8114596.1"
                     /translation="MRVIDTWQTAPPSDDSEQGGLELTQSAHLRILAGACSQPAYPVE
                     MQGLSIGREECDVVIADDPRISRKHARVERSFAGWQFQDLGSRNGFYVNGRGFGRGQR
                     TPLPDGSVIRIGDSVMVFRSTALPSDPRIDSAVFPGVSPEAANVRRRIDELAGAFGHV
                     LILGETGTGKESVAKAIGAQRAPHPFVTLNCAQLNREMARSELFGHVRGAFTNAVGTR
                     PGLVDLAGEGALFLDEIGDLPLDVQAELLRFLEDGSYRALGSTDLRKSSARVVAATNI
                     DLDHAVLQARFRRDLLARLRATNTPLTLPPLRERREDILGWTQLFFRLRGRDPGPRPW
                     TAGALECLLLYPWQENLRELLAAVNFAASQTPEFPCTRAQLPAQVRAHRSGLRMPADS
                     RPDDPTPAPRPSPAPPPPEEDLPPRADPTRDEIIEVLGQTRGRMRTTAQLLKIDRRRL
                     YRLCKAYDINPDDHRDRHDPEND"
     gene            5088..6518
                     /gene="glmU"
                     /locus_tag="VF516_43000"
     CDS             5088..6518
                     /gene="glmU"
                     /locus_tag="VF516_43000"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEX8114597.1"
                     /translation="MSRTAPLVFVMAAGLGTRMKSDLPKVLHRIAGRPLLHWVVTSAR
                     AAGAGRVIAILGHKADAVQASLGAAFGAGAVEVAIQSEQRGTGHAVQCALPAVAAEPD
                     DRIAVILSGDAPLLGSERIAELALACEASPAGMALLSTVPPRPMPYGRLVRETGGKLL
                     RIVEDADATEDQRKIRDTNAGFYAIRLGHLRHDLARLRADNAKGELYLTDLVAHAAAR
                     GGATAIDAPFAEVAGINDRVDLAASEAVARRQINEAWMRAGVTMADPATTYIDAEAGP
                     IGRDVWLGPNVALRGKTSIGDAARIDTGSVLTNVQVADHAYLKPYSVLTDTAIGPRAE
                     IGPFTHCRPGSRIDEDAKLGNFVETKKTHMMAGAKANHLAYLGDASVGAKANIGAGTI
                     TCNYDGVSKHQTTIEAGAFIGSDTQLVAPVTVGRDAYVGAGTTVTKDVPRGSLALTRV
                     KQVNVEGWADRFREAQAKRKKASDEH"
BASE COUNT         1016 a         2374 c         2266 g          957 t
ORIGIN      
        1 gctggtcgag atcgcccacg agtcgctggt ctagacctgg tcgcagctcg cgcgctggct
       61 cgacgagtcg cgcgaggagc tccggctgct cgaggagctg accgacgcgg ccaagctgtg
      121 ggcgcgccgg ggcaagcgca gcgagcacac ctggtccgag cgcgacatcg cgaccgcccg
      181 gcatcgcgcg gcccagctcg gcctcgcgct gccgccgcgg atcggggagt tcttcgccgc
      241 cggcgatcag cgcaaccggc tggcgcggcg gcgccgctgg atccgcattg gcatcacgtt
      301 cgccatggcg agcgccgtgg ccgtgttgct gctgctcaag ttcgtccagg accgctggcg
      361 agatgagttg atccggaaca acctcggcac cgtcatctac accatcgtcc cgttcgacgg
      421 gcgcgatggc gcgatcgtcc cggcgaccgg tgatccgatg cccggcctca ccgtgacggt
      481 gtacggcgcc aaggccgatg acacccatga accgggagac ccgattccgg acaaacttgt
      541 ccgcatcgtg gccatgggag gcgccggcaa cccgcgcgtc cagcggatca gcgcccccgg
      601 cggcacggtg ttcctgcggc ttgacggccg cggtcggccc ggggagcact gcccgccgtc
      661 gtggatccgc gtccaggcat ttcccggcta ccggatcgac gacatcaagc ggatcgagct
      721 cccggtgccg acctgccagg ccaccctggc ggacaccgtg aagatcgacg ccggcccgtt
      781 ctggtatggc ggacccggcg agccgcccgc gaagcattat ggcgagcccg actacaccga
      841 gcctgagcga tcgatcgatt tgccggcgtt tgccatggac cgcaccgagg tatccaatgc
      901 cgcattcgag ccctttgcgc ggctggaaaa actgaccggt tacccgaggc cgatctacag
      961 ccgggacggc gtgaacatcc acgacggcga gccgtcctac ccggtcagcg atatcgatgc
     1021 cttcgaggcc gctgcgtatt gccggtacat gggcaagcgg ctgccgagcg acgccgagtg
     1081 gaccaagtcg gcgcgcggcg ggctgacggt gaatggcaag cccaatccag ctccgcgccg
     1141 gctgtatcca tggggaaaca cctggaagcc ggaatgtgtc aaccaggact atgccgtgaa
     1201 caagcaggcg tggacccgtc cggtcgattc gctggcctgc ggcgcgagcc cctacggcat
     1261 cctgcagctg gtcgggaacg tgcaggagtg gatcgctcgt gatggccaga ccgaccggga
     1321 caacccgctc catgccctgc ggggtggttc gaccgacgac gctcccggtg acgagaccac
     1381 cacgacggtg tttcgcaatc accgcgaccc tcgcgtcctc aattattcca acggcgcgag
     1441 atgcgtcgtc gatctggaag gtgcaccatg atactcagcg atcagcccag gaccgtgacc
     1501 ctggtggcca atccaccacc cgagaccgac aacggcggtc tggatcagac cttccgggtg
     1561 ctgtccacca agggcgtccg tgacaacgag atccgccggg ttgcgagtgt cagtgatatc
     1621 gacatcgcgc tcagcaacgt gttgacgccg gcgttcaagg ccacggtgcg actgcagatc
     1681 atcggacacg cgatcccggg atcgatgtgc ctcggcgcga agtggaccga caccgggatc
     1741 cacgatcccg ccgcgttcgc gttcccgttc ctcgtgctcg ataccaatcc caccgcgctg
     1801 ggcctgctcg caaagtttgc cggcaagctg tcggaggtga tgctcgtggg ctgtcatgtc
     1861 ggatcgacga cgtcgttcgg acacgcgatc aacggacgga ccctgacgta cacgctggcc
     1921 gagctgctgc gttgcaaggt gtgcggcgcc gacgacgacg tcgcgcccga cgagtacgac
     1981 gaccagggct ggtacgcgcc cgcgccgcag catcgccgcc cgaagggctg gcggtggagc
     2041 gagtcctcgc cgccgctgtg gatcgatctc ggcacggatc cgctgccgcg caaccgcaag
     2101 ccggcgggcc ggaacggcaa gccgctcgag atcgagatcc ggacgatccg gaccgcgatg
     2161 ctcccgctgc tcgcgtcgcc gcagccggtc gatccgtcgc tcgatctccc ttcgtccctc
     2221 cgggtcacct gccagcacac caccgccagg ccggtctcgg cgttgcccga gctgtcggtg
     2281 gacaccgatc tcgggccggc gcagatcctg tgcggcggcc gttacctgac catcgccgat
     2341 gtgtgctacg tcctcgagcg caatccgcca ctgactgcgg cgctcaccgc gctcccgcgg
     2401 accatcgatg ccgcgctccg cgcgccgacc accgcggcgc cccccggcgg ccgcgcggtg
     2461 cccgccgccg gctagcccgg gctagcccgg actcaccggc aaccgttgcc cgtggccgtc
     2521 cacacccggc tacatgcccg ggacgacgtg gaacgcgccg aagccattct gggtcaggac
     2581 cagcgagatc ggcgtaccgc gctgtgcaaa ctggatatgc tgcgcatgcg cggcgaggtt
     2641 accgtcgagc agatcgacct ctcccgagat cgccggcgcg ctcttggcgc tgccggacac
     2701 gaggatctgc gtcggccata ccttcaccgt gccgtcgtgc gggtccgccg acggtccact
     2761 ggcgtgctcg gccagccgga tctccaggta ctccgagcca tgccaccgca gatcctgcga
     2821 ctccgcggcg tcgggtccga gcaggtcaat ctggaccagc tcactcttgc cctcgggata
     2881 cccctgcacc accagcttgc tcgggatcac cgtcagatcg ccctggaagg gcgcagcggc
     2941 caggcaggcc ggcagcttga aggcgacggc atcggtgccg gccccgagga tgtggtagcc
     3001 atccggtgtc gtagtttgcc agccagtctt cgacaggaac agcggccccg tcgcacccgg
     3061 cttcatctcg accggcgccg gctgacccgg aatgggcgca gtccatactt ccttcaccgc
     3121 aaccgtgaac aagcgccgca gacgctcgtt cgggtcaccg gtcttactat cactacgtgt
     3181 ccagaaactg acgttctgag cgtccactga ctcgatcgtt acctgcggga ccatggcatg
     3241 cgtgcactgc atcgacttcc tccagagccg cggaggagct tcaagagcga tgccaacgaa
     3301 ccgccgcgcg gtgggtcagt cgttctcggg gtcgtgccgg tcgcgatggt cgtctgggtt
     3361 gatgtcgtat gccttgcaca gccggtagag ccggcggcga tcgatcttga gcagctgcgc
     3421 cgtggtccgc atccggccgc gggtctgccc cagcacctcg atgatctcgt cgcgggtcgg
     3481 atcggcgcgc ggcggcagat cttcctcggg gggcggcggt gccggcgacg gccgcggcgc
     3541 gggcgtcggg tcgtccggtc gcgagtcggc cggcatccgc agcccgctgc gatgcgcgcg
     3601 aacctgggcc ggcagctggg cgcgcgtgca cgggaactcg ggggtctggc tcgcggcgaa
     3661 gttcacggcg gcgagcagct cgcgcaggtt ctcctgccag gggtacagca gcaggcactc
     3721 cagcgcgccc gcggtccacg gccggggccc ggggtcgcgg ccgcgcaggc ggaagaacag
     3781 ctgggtccag cccaggatgt cctcgcgccg ctcgcgcagc ggcggcagcg tgagcggcgt
     3841 gttggtcgcg cgcagccgcg ccagcaggtc gcggcgaaac cgcgcctgga ggaccgcgtg
     3901 atcgagatcg atgttggtcg cggcgaccac ccgggccgag ctcttgcgca gatcggtcga
     3961 gcccagcgcg cggtacgagc cgtcctcgag gaaccgcagc agctcggcct ggacgtcgag
     4021 cggcaggtcg ccgatctcgt cgaggaacag cgcaccctcg ccggcgagat cgaccaggcc
     4081 gggccgggtg ccgaccgcgt tggtgaacgc gccgcggacg tggccgaaca gctccgagcg
     4141 cgccatctcg cggttgagct gggcgcagtt gagggtcacg aacgggtgcg gcgcgcgctg
     4201 ggcgccgatc gccttggcga cgctctcctt gccggtgccg gtctcgccga ggatcaggac
     4261 gtggccgaac gccccggcca gctcgtcgat ccgccgccgc acgttggccg cctccgggga
     4321 caccccggga aacaccgccg agtcgatgcg cggatcggac ggcagcgccg tgctccggaa
     4381 caccatcacg ctgtcgccga tccggatcac gctgccgtcc gggagcgggg tgcgctggcc
     4441 gcgcccgaag ccgcggccgt tgacgtagaa cccgttgcgg ctgcccaggt cctggaactg
     4501 ccagcccgcg aagctgcgct cgacccgggc gtgcttgcgc gagatccgcg ggtcgtctgc
     4561 gatgaccacg tcgcattcct cgcggccgat gctcagtcct tgcatctcga cgggatacgc
     4621 cggctgggag caggccccgg cgaggatgcg gaggtgcgcg ctctgggtca gttccaggcc
     4681 gccctgctcg gaatcgtccg acggcggtgc ggtctgccag gtatcgatca ccctcatcgg
     4741 catcacgcta tccaggctgt tgaagggcgt ccgactggac cgattttcca cagcctatcg
     4801 cacaccatgg ggtgcccctc cccgacccgg aagccgcgat gcgagtgact ctgcgcatgc
     4861 gccgcgcatg cgcctcccga tccgcgcatg gcgctctacg catctgtgca gctgtcggct
     4921 ttctgaacgt atcgacagcc ctgtgtaaca cacccgggca ccgcacgtct tccgcacacg
     4981 gccgcgacct cccacccgcg gtgctcgcgc atgtgcgctg acgcccgggg ccaacggcgg
     5041 caggagcggc gatccgcacc ttcaggaatg ctaccgttct gccgtacatg agccgaaccg
     5101 caccgctggt gttcgtcatg gctgctggtc tcgggacgcg gatgaagagc gaccttccca
     5161 aggtcttgca ccgcatcgcc ggccggccgc tgctccactg ggtcgtgacc tcggcgcggg
     5221 ccgcgggagc gggtcgcgtg atcgcgatcc tcggccacaa ggcggacgcg gtgcaggcgt
     5281 cgctcggcgc ggcgttcggc gccggcgcgg tcgaggtcgc gatccagtcc gagcagcgcg
     5341 ggaccggcca cgcggtgcag tgcgcgctgc cggcggtggc cgccgagccc gacgaccgga
     5401 tcgccgtgat cctgagcggc gacgcgccgc tgctcggcag cgagcggatc gccgagctgg
     5461 ccctggcgtg cgaggcatcg cccgccggca tggcgctgct gtcgaccgtg ccgccgcgcc
     5521 cgatgccgta cggccggctg gtccgcgaga ccggcggcaa gctgctgcgg atcgtcgagg
     5581 acgccgacgc caccgaggat cagcgcaaga tccgcgacac caacgccggg ttctacgcga
     5641 tccggctcgg ccacctgcgc cacgacctgg cgcggctgcg cgccgacaac gccaagggcg
     5701 agctgtacct gaccgacctg gtcgcccatg ccgccgcgcg cggcggtgcg accgcgatcg
     5761 acgcgccgtt cgccgaggtc gccgggatca acgaccgcgt cgacctcgcg gcgagcgagg
     5821 cggtcgcgcg ccgccagatc aacgaggcgt ggatgcgggc cggcgtcacg atggccgacc
     5881 cggcgaccac gtacatcgac gccgaggccg gcccgatcgg ccgcgacgtc tggctcggcc
     5941 ccaacgtcgc gctgcgcggc aagacgtcga tcggcgacgc cgcgcggatc gacaccggct
     6001 cggtgctcac caacgtccag gtcgccgacc acgcgtacct caagccatac tcggtgctga
     6061 ccgacaccgc gatcggcccg cgcgccgaga tcggcccgtt cacccactgc cggccgggca
     6121 gccggatcga cgaggacgcc aagctcggca acttcgtcga gaccaagaag acccacatga
     6181 tggcgggcgc gaaggccaac cacctggcgt acctcggcga cgcctcggtc ggcgccaagg
     6241 ccaacatcgg cgccggcacg atcacctgca actacgacgg cgtcagcaag caccagacca
     6301 cgatcgaggc cggcgcgttc atcggctcgg acacccagct ggtcgcgccg gtcacggtcg
     6361 gccgcgacgc ctacgtcggc gcgggcacca ccgtcaccaa ggacgtgccg cgcggctcgc
     6421 tggcgctgac ccgcgtcaag caggtcaacg tcgagggctg ggccgatcgg ttccgcgagg
     6481 cccaggccaa gcgcaagaag gcgagcgacg agcactgatg ctccgtgctc atgcaggccg
     6541 gctcgcgctc gccgtggcgc tggccgcctg caaggaccgc cacgcgccgc acgacgacgc
     6601 cgcgccgcgg gcc
//
LOCUS       DASVOR010002333         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_55429,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002333 DASVOR010000000
VERSION     DASVOR010002333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55429"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VF516_43005"
     CDS             <1..1077
                     /locus_tag="VF516_43005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEX8114598.1"
                     /translation="ALEKAMAMIDYRALRAEQLEKRKKGQLMGIGISSFTEVVGAGPS
                     KDYDIIGIKMFDSAEIRIHPTGKAMARFGTKSQGQGHETTYAQIVAEELGLPAADVQV
                     EEGDTDTAPYGLGTYASRSTPTAGAAAAMAARKIKAKAKKIAAYLLEVGEDDLEWHVD
                     HFRVKGSPDKKKTIQEIAFAAYTNHPQGMEAGLEATDYYDPPNLTFPFGSYICVVDLD
                     TGTGEIKIRRFVAVDDCGHIINPMIVEGQIEGGLTMGLAPAMFEEIVYDANGQNLSGT
                     LADYLVPTAVESPRWELGKTVTPSPHHPIGAKGVGESPTVGAPPAIANAIVDAMWHLG
                     VRHIDIPITPQKVWRLLRKHGVTE"
     gene            1445..1621
                     /locus_tag="VF516_43010"
     CDS             1445..1621
                     /locus_tag="VF516_43010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114599.1"
                     /translation="MKRSLLVSVAVLVVSLFVGTALKVQAATTSAPAAASAAVQADAS
                     GPGYWAAWAEAASH"
     gene            1727..>2077
                     /locus_tag="VF516_43015"
     CDS             1727..>2077
                     /locus_tag="VF516_43015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114600.1"
                     /translation="MIKKRLSAFERRFGYDATYMRELLATDARAFLRYARIAGLLKYR
                     RDVPTDVYYAASLTAIVAEDCGPCSQLTVAMALGDGVDPRTVAAVVGGNEAEIAEPAR
                     LGVRFARAVLAHDPA"
BASE COUNT          314 a          738 c          747 g          278 t
ORIGIN      
        1 gcgctcgaga aggcgatggc gatgatcgac taccgggcgc tgcgcgccga gcagctcgag
       61 aagcgcaaga agggccagct gatgggcatc gggatctcga gcttcaccga ggtcgtcggc
      121 gccggcccgt ccaaggacta cgacatcatc ggcatcaaga tgttcgactc ggccgagatc
      181 cgcatccacc cgaccggcaa ggcgatggcg cggttcggca ccaagtcgca gggccagggc
      241 cacgagacca cctacgccca gatcgtcgcc gaggagctcg gcctgcccgc ggccgacgtc
      301 caggtcgagg agggcgacac cgacaccgcg ccgtacggcc tgggcaccta cgccagccgc
      361 tcgacgccga ccgcaggcgc cgccgcggcg atggccgccc gcaagatcaa ggcgaaggcc
      421 aagaagatcg ccgcctacct gctcgaggtc ggcgaggacg acctcgagtg gcacgtcgac
      481 cacttccggg tgaagggctc tcccgacaag aagaagacga tccaggagat cgcgttcgcc
      541 gcgtacacca accatccgca gggcatggag gccggcctcg aggccaccga ctactacgat
      601 ccgccgaacc tgacgttccc gttcggcagc tacatctgcg tggtcgatct cgacaccggc
      661 accggcgaga tcaagatccg gcggttcgtc gcggtcgacg actgcggcca catcatcaac
      721 ccgatgatcg tcgagggcca gatcgagggc ggcctgacca tgggcctggc gcccgcgatg
      781 ttcgaggaga tcgtctacga cgccaacggc cagaacctgt cgggcacgct cgccgactac
      841 ctggtgccga ccgcggtcga gtcgccgagg tgggagctcg gcaagacggt cacgccgtcg
      901 ccgcaccacc cgatcggcgc caagggtgtc ggcgagtcac ccaccgtcgg cgcgccgccc
      961 gcgatcgcca acgcgatcgt cgatgcgatg tggcacctcg gcgtccgcca catcgacatc
     1021 ccgatcacgc cgcagaaggt atggcgcctg ctgcgcaagc acggcgtgac cgagtgaccg
     1081 ccggtccggg ccgggttctc cgcgacggca ccggaacagt ggccccatgg cccggaccac
     1141 gcggcggtcc acctggaccc tgcgatgtcc aggtggcgcg gtggcaggtc gggcgggcga
     1201 gggccggcgt cgccgcgcgg tcgcgcatcc ctcccgcgct cctcccgcgc tcgcgagcgt
     1261 ggccggggcg cgcgatccgg tgggccccgt gcggagatgt cgagctcacc ggccgcccgg
     1321 ggccgcctct gggccgcgat gtggagcgcc cggaggcgtg ccgcggttcg cggcgaggcg
     1381 ccgcgcttcg cgtcgctccg gatgcgcgcg acgggtggca cgtcggttgc gaagagctcc
     1441 gagcatgaag cgctcgctcc tggtctcggt cgctgtcctg gtggtttcgc tgttcgtcgg
     1501 tacggcgctg aaggtgcagg cggcgacgac gtcggccccg gccgctgcgt ccgcggccgt
     1561 gcaggcggac gcctcgggcc ccggctactg ggcggcctgg gcggaagccg cgtcgcactg
     1621 aggggccgcc gggcgcgaac gcgattccgc gggtttcgcg ccgatgtcac gggggtgcgc
     1681 gtcgcctcgt ccttcgatca ggaggacacc cccgatgatg acgtggttga tcaagaaacg
     1741 gctgagcgcg ttcgagcgca ggttcggcta cgacgcgacc tacatgcgcg agctgctcgc
     1801 gaccgacgcc cgggcgttcc tgcgctatgc ccggatcgcc gggctgctga agtaccgccg
     1861 cgacgtgccc accgacgtgt actacgcggc gagcctgacc gcgatcgtcg ccgaggactg
     1921 cggcccgtgc agccagctca cggtcgcgat ggcgctcggc gacggcgtcg atccgcgcac
     1981 ggtcgcggcc gtggtcggcg gtaacgaggc ggagatcgcc gagccggcgc gcctcggcgt
     2041 ccggttcgcg cgcgcggtgc tcgcccacga tccggcc
//
LOCUS       DASVOR010002334         6115 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_230106,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002334 DASVOR010000000
VERSION     DASVOR010002334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230106"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..428
                     /locus_tag="VF516_43020"
     CDS             <1..428
                     /locus_tag="VF516_43020"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8114601.1"
                     /translation="EAAPIYDRLAEDAKAGRRMKDVARFRQRQGGILEARGDRAGALT
                     AYEEALRVNPTDVTTMTGLGRLYFAAQDWEKARKIYQSLVLQNVDAEAGVTKAEVYWA
                     LGKIHLQLGQPPKAKSMFQRGLEIEPHNSKLREALSALQ"
     gene            625..1338
                     /locus_tag="VF516_43025"
     CDS             625..1338
                     /locus_tag="VF516_43025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114602.1"
                     /translation="MSGSARAEIEHEIGAPTPPGPGLDRAQVRGIPWCGSVHERDPEW
                     WMHIVDELHQYRTSRYMEHRLFQLARGVCSAPDDPLAQRIATEVLQLWINQTGMSAPD
                     AVASLSLRADKDAFQAERTALCDAIKPKGDDPRKERTALAAARFLLLGCKREAPLWMF
                     GDEVEHLGPFIDRGSAARDDLAHLAWVLHRQYGDMEEGNSNALAGYAIDQFDFHAVSA
                     DAALHQLGAAPFRGSRYAR"
     gene            1328..1783
                     /locus_tag="VF516_43030"
     CDS             1328..1783
                     /locus_tag="VF516_43030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114603.1"
                     /translation="MPGDRAESLGRAQLVTAKIEALVAKRASDSEWKELLITAPDQVC
                     RETNKIDSQDPKTGKITYRQVCHYTGAHMVEVGPDPVDVPVELAAGLQPGRYARFSRI
                     SIEGHMRSSPSAPKLPLEVYRGVQKQKDDFDRERESGKHLIALYGFLLE"
     gene            complement(2083..2298)
                     /locus_tag="VF516_43035"
     CDS             complement(2083..2298)
                     /locus_tag="VF516_43035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114604.1"
                     /translation="MVDIEALAHAAEAVADALPAPATACQRVLSGRIQSLATKTSQQA
                     SASLRFANAQIIALEAQMEAHRNTPAR"
     gene            2851..3624
                     /locus_tag="VF516_43040"
     CDS             2851..3624
                     /locus_tag="VF516_43040"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8114605.1"
                     /translation="MTTSTAVIMLLHMAAKSKRARKRHVQQQLFRRGGERRGAGRKPK
                     GARAGERHQARSAFKAYHALHVVMRVAPEVGSLRRRAMYKALRNATITAALRERFRIV
                     HISLQRTHVHMIVEADDKAALARGMQGFTISAARHINTALGNGGRRRRGPVFADRYHV
                     EVMTTPTRARHAVSYCLNNWRKHGEDRGGLASTWLVDPFSSGISFPDWKELQDQAWMW
                     PIRETYDPLMVRRPATWLLAEGWKLAGEISACEVPSRRR"
     gene            complement(3684..4178)
                     /locus_tag="VF516_43045"
     CDS             complement(3684..4178)
                     /locus_tag="VF516_43045"
                     /inference="COORDINATES: protein motif:HMM:NF021425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2127 domain-containing protein"
                     /protein_id="HEX8114606.1"
                     /translation="MPRPSRDILIRLIALFKLFKALLLIAIGIGALSLRHNHDGALAG
                     WIHALVFDPHGRLLHEAVAKISGLDTRQLEGIAIGSLVYAAVFLVEGVGLMLRRGWAE
                     VLTTVITVSFIPLEIYELVKHESWAKIGVVVINALIVVYLVRRLRREGHWPFHRRRAL
                     GELR"
     gene            complement(4406..5029)
                     /locus_tag="VF516_43050"
     CDS             complement(4406..5029)
                     /locus_tag="VF516_43050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114607.1"
                     /translation="MNLFEEDPAPIHLPEAVAGHEAGHAVLAYYHREQIGPTHFDWYG
                     KELGGRHLHGPPPGITDERSHVDAAVERLVAGDLGGRIVAGLPLNRVSIPVARAFLIG
                     PWTSVTALAQRIHDARNGEGRHDVVKVMAAIASYKDHKVLWKRRWWRWFWERVRRSRA
                     VLTSEDGTRAHRLLSAMFHRAIGPSGGVVPGEITVELLRQARAPRGP"
     gene            complement(5039..5869)
                     /locus_tag="VF516_43055"
     CDS             complement(5039..5869)
                     /locus_tag="VF516_43055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEX8114608.1"
                     /translation="MLTFNDSLRTVNLDPAVVRLVRHRDPNVQRAVFEAAIKGDPAFD
                     EYQEHQGTDQVIAQFRAATYLAGFVADPMTNETIFVGIWERLGDSPGPSARIPLTIAG
                     THSAAVAFKTKRTDMLAAYRGRIVIGWGDGARVWVQRADNQPKPILEIRRELRDPDFP
                     GFARFCRQLDQIEQVPLSWRAVLSNARGIYLIVHRESGQQYVGSAYGVEGFFGRWANY
                     ADGHGGNIGLKELGASASAFNVAVLEVAGSEATNEEIFARETLWKEKLGTRVKGLNRN
                     "
BASE COUNT         1058 a         1981 c         2061 g         1015 t
ORIGIN      
        1 acgaggccgc gccgatctac gaccggctcg ccgaggacgc caaggccggc cgccggatga
       61 aggatgtcgc gcggttccgc cagcgccagg gcggcatcct cgaggcccgc ggcgaccgcg
      121 ccggcgcgct gaccgcgtac gaggaggcgc tgcgggtcaa cccgaccgac gtcaccacga
      181 tgaccgggct cggccggctg tacttcgccg cccaggactg ggagaaggcg cgcaagatct
      241 accagtcgct ggtgctgcag aacgtcgacg ccgaggccgg tgtcaccaag gccgaggtct
      301 actgggcgct cggcaagatc cacctccagc tcggccagcc gcccaaggcc aagagcatgt
      361 tccagcgcgg cctcgagatc gagccgcaca actccaagct gcgcgaggcg ctgtccgcgc
      421 tgcagtagcg ccgccggcgg ttcgcgcccg gccgtccggt cggagggcgt gccgccgcgc
      481 ggggcatggt gcccagaaat tttttggtgg ttttctgcgc ggaccgccga ccggaggaaa
      541 catgacccac gacgaccgac gagctggatc ctcgtgctct gcgcgctgct ggcggtggga
      601 tcgaccgcgc gggctcagca ggccatgtca ggctctgcgc gcgccgagat cgagcacgag
      661 atcggcgccc cgactcctcc cggacccggg ctcgaccgag cgcaggtccg cggcatccca
      721 tggtgcggca gcgtgcacga gcgcgatccg gagtggtgga tgcatatcgt cgacgaactc
      781 catcagtatc gcaccagccg atacatggag caccggctgt tccagctcgc acgcggggtg
      841 tgcagcgcac cggacgatcc gctggcgcag cggatcgcga ccgaggttct ccagctgtgg
      901 atcaaccaga ccgggatgtc ggcgccggat gcggtcgcgt cgctgtcgct gcgcgcggac
      961 aaggacgcgt tccaggccga gcgcaccgcg ctgtgtgatg cgatcaagcc caagggcgat
     1021 gatcctcgca aggagcgcac cgcgctcgca gcggctcggt tcctgctgct cggctgcaag
     1081 cgcgaggcgc cgctgtggat gtttggcgac gaggtcgagc acctggggcc gttcatcgat
     1141 cgcggctcgg ccgcgcgcga cgacctcgcc catctggcgt gggtgctgca ccgccagtac
     1201 ggggacatgg aggagggcaa cagcaacgcg ctcgcgggat atgcgatcga tcagttcgat
     1261 tttcatgcgg tgtccgccga cgcagcgctc caccagctcg gcgccgcgcc gttccgcggc
     1321 agccgctatg cccggtgatc gtgctgagtc gctcggccgc gcgcagctgg tcaccgctaa
     1381 gatcgaggcg ctggtcgcca agcgggccag cgattcggag tggaaggagc tgctgatcac
     1441 cgcgccggat caggtctgcc gcgagaccaa caagatcgac agccaggatc ccaagaccgg
     1501 caagatcacc taccgccagg tgtgtcacta caccggcgcg cacatggtcg aggtcgggcc
     1561 cgacccggtg gacgtgccgg tcgagctcgc cgccgggctc cagcccgggc gctacgcgag
     1621 gttcagccgg atctccatcg agggccacat gcgctcgtct ccgtcggcac ccaagctccc
     1681 gctggaggtc tatcgcgggg tccagaagca gaaggacgac ttcgatcgcg agcgcgaatc
     1741 cggcaagcac ctcatcgcgc tctacggctt cctgctggag tagctcagcg cagctcgccg
     1801 agccggtggc ggcggtggaa cggccagtgg ccctcacgct tgaggcggcg caccaggtac
     1861 acgacgatca gcgcgttgac gatgacgccg ccgctctttc gatcagctcg tagatcttca
     1921 ccgggatgaa cgaggtcggg gagaacgcgc ggtcgcctgt gactggctcg tcgaagctaa
     1981 ccaggtcgac caggacgacc cactcacagt cgggtgcccc gcacgaaggc tcgcagctca
     2041 cggatatttt gtcttgaatt ccggaagggg agctcgacag tctcagcgag cgggcgtgtt
     2101 tcggtgggcc tccatctgcg cctcgagcgc gatgatctgc gcgttcgcaa agcgcagcga
     2161 agcgcttgct tgctgtgacg tcttcgtggc gagcgactgg atccgaccag acagcacgcg
     2221 ctggcaggcg gtcgccggtg ccggtagcgc gtcagcgacc gcctctgcgg cgtgggcgag
     2281 cgcctcgatg tcgaccacct cggcgcggag gttctccagc gtggtcgcgg aatcgaagtt
     2341 gctgtggacg ggctcgttcg ttacctgacc tgggtgctgc gatgctcggt gaggcaattg
     2401 aatccaattc tacgaatcgg gatcaataaa tgaactctgc cccgcggtgt tgagtcgagg
     2461 gcaagggctg tgggcaacgg aaggaggctt gtgagccgcg cgtgggtcag cctggtgcgc
     2521 cacggcgcgg gtcatcaagc cgacccgttg tccacagacc gtcgagatgt tctgcagcga
     2581 tcctcgatcg agattcgtcg gagccgcgca ccgtcgagcg tggcggctgc ctggtagcac
     2641 ccggcgatcc gcgaccaggg cgcgaggccg ggtgatgtcc gctgatcgga tcgacaggat
     2701 cgagccagat tgcagtcgat gcctcggcgc cgaggtggaa accctgtcgt ctatcgagta
     2761 tgattgtatc caaatctcta caaagtagtc agggttcaca aatccttcta acattttgat
     2821 tattgactgt aattagttct gccaaatcaa ttgacaacca gtacggcagt gataatgctt
     2881 ctgcacatgg cggcgaagtc gaagagagcg cgcaagcggc acgtccagca gcagctgttc
     2941 cggcggggag gcgagcgacg cggcgccggg cggaagccga agggcgcgcg cgccggggag
     3001 cggcaccagg cgcgatcggc gttcaaggcg taccacgcgc tgcacgtcgt gatgcgggtt
     3061 gcgccggagg tgggcagcct gcgtcggcgc gcgatgtaca aggcgctgcg caatgccacg
     3121 atcaccgcgg cgctgcgcga acggttccgg atcgtgcaca tcagcctgca acgcacgcac
     3181 gttcacatga tcgtcgaggc cgacgacaag gcggcgctcg ctcgggggat gcaaggcttc
     3241 acgatctccg ccgcgcggca catcaacacc gcgctgggca acggcggcag gcggcgccgc
     3301 ggtccggtgt tcgccgaccg ttatcatgtc gaggtcatga ccacgccgac gcgtgcccgc
     3361 catgcggttt cgtactgctt gaacaactgg agaaagcatg gcgaggaccg tggggggctg
     3421 gcgagcacgt ggctcgtgga cccgttctcc tcggggatct cgttcccgga ctggaaggag
     3481 ctgcaggatc aggcgtggat gtggccgatc cgggagacct acgatccgct gatggtgcgg
     3541 agacccgcga cgtggctgct ggcggagggg tggaagctcg ccggggagat cagcgcctgc
     3601 gaggtgccga gccggcggcg gtgaccgacc gctccggatc gcggccagcg cgattgccag
     3661 acgcgacgaa gcggcggcgg cggtcagcgc agctcgccga gcgcgcggcg gcggtggaac
     3721 ggccagtggc cctcgcgcct gaggcggcgc accaggtaca cgacgatcag cgcattgatc
     3781 accacgacgc cgatcttcgc ccagctctcg tgcttgacca gctcgtagat ctcgagcggg
     3841 atgaacgaga ccgtgatgac cgtcgtgagc acctcggccc agccgcggcg cagcatcagc
     3901 ccgacgccct cgaccaggaa caccgccgcg tagaccaggc tgccgatcgc gatgccctcg
     3961 agctgccggg tgtcgagccc gctgatcttc gcgaccgcct cgtggagcag cctgccgtgc
     4021 gggtcgaaca ccagggcgtg gatccagccc gcgagcgcgc cgtcgtgatt gtggcgcagc
     4081 gacagcgcgc cgatgccgat cgcgatcagg agtagcgcct tgaacagctt gaacagcgcg
     4141 atcaaccgga tcaggatgtc gcgcgagggt cggggcattc ctaactatga cgcgcgcggc
     4201 gtggacgtgc acctcgcggc gtggcgcggt gcgccatcgc gtcggcgccc tccaatcgtc
     4261 aatttaccag gcacccggct gcgaccatcg cagcccgggt acctcgctgt gccgttcact
     4321 cgcggagtcg tttcccttga cggatcacgc catgaatcgc tcaccaagga cctcgtcagg
     4381 ctggctcatt cgcgcatcgc aagggtcatg ggccacgcgg tgctcgcgct tgtcgaagaa
     4441 gctcgaccgt gatctctcct ggaacgaccc cacccgaggg tccgatcgcg cggtgaaaca
     4501 ttgcacttag tagccggtgc gcacgtgtgc catcttctga tgtgagtacc gctctcgatc
     4561 ttcttacgcg ttcccagaac caccgccacc atcgacgctt ccaaagtacc ttgtgatcct
     4621 tataagacgc gatggctgcc atcaccttga caacgtcgtg ccggccttcg ccattgcggg
     4681 cgtcgtgaat gcgttgagcg agcgctgtca ccgaggtcca tgggccgata aggaaggccc
     4741 gcgcaacagg aatcgacaca cgattcaaag ggagtccggc aacgattcgg cctccgaggt
     4801 cgccggcaac caggcgttcc accgcggcat ctacgtggct acgttcgtcg gtgattcctg
     4861 gcggtgggcc gtgaagatgt cgcccgccga gctctttgcc gtaccagtcg aagtgagtag
     4921 ggccgatctg ctctcggtgg taataggcca gcacggcgtg tccggcttcg tggccggcca
     4981 ctgcttctgg caaatgaatg ggcgctggat cttcttcgaa aaggttcatt tggcagcttc
     5041 agttgcgatt cagacctttg accctcgtgc ccagcttttc cttccaaagg gtctcgcgcg
     5101 cgaagatctc ttcgttcgtc gcctcgctgc ccgcgacttc caggaccgcg acgttgaagg
     5161 cgctcgcact cgcacccaat tctttgagac cgatgttccc gccgtggccg tcagcgtaat
     5221 tcgcccagcg tccgaagaag ccctcaacgc cgtacgccga cccgacatac tgctgcccgc
     5281 tctcgcggtg tacaatcagg taaatgccgc gcgcattgga caacacggcc cgccacgaga
     5341 gcggcacctg ctcgatctga tcgagctggc gacaaaaccg tgcgaacccc gggaagtcag
     5401 gatctcgaag ctctcgccgg atctcaagga tcggcttggg ctgattgtcg gcccgctgga
     5461 cccatacgcg cgcaccgtcg ccccagccga tcacgatcct accgcggtag gcggcgagca
     5521 tgtcggtgcg tttggtcttg aatgcaacgg cggcgctgtg cgtgcccgcg atcgtgagcg
     5581 ggatccgtgc cgaggggccc ggactgtccc cgaggcgctc ccagatgcca acgaagatcg
     5641 tttcgttcgt catcggatcg gcgacgaagc cagcgaggta tgttgcagca cggaactgcg
     5701 cgatcacctg atcggtgcct tggtgctcct ggtactcgtc gaacgcaggg tcgcctttga
     5761 tggcggcctc gaacacggcg cgctggacgt tgggatcgcg gtggcgcacg agtcgcacca
     5821 cggccggatc cagattcact gttcgtagcg agtcgttgaa cgtcagcacg tgcaccctcc
     5881 agatgaccaa cgtaccatta ccgcggacct gcgggatgac ctcccatgct gcgatgagct
     5941 cccgcgcgac gggagaattg atcgaccgag atgtcgcgtg aacaatgcgg tcaatagcca
     6001 cggtacgtcc ggcccgcgac cagccacggc gcggccccgc cgggcggctg cgccacgagg
     6061 tagaccggat cgccggcgag cggcgcggcg agcgtccagc cggtggcggt cgcgg
//
LOCUS       DASVOR010002335         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_171549,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002335 DASVOR010000000
VERSION     DASVOR010002335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171549"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="VF516_43060"
     CDS             complement(<1..458)
                     /locus_tag="VF516_43060"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8114609.1"
                     /translation="MPSGRPLITANQVTLARLIPMPLLSWLIYRGAEQGYKHNAYMTS
                     ALIAGTVIGCTDWIDGLLARKYGPTVLGGLLDPIADKIFIAFAYTPFVDVARPLVPWW
                     MVALLFQREFFVTALRSAYEQRDLTLKTSYLAKAKTWTQMQGIGVILLFPL"
     gene            complement(562..2016)
                     /locus_tag="VF516_43065"
     CDS             complement(562..2016)
                     /locus_tag="VF516_43065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114610.1"
                     /translation="MIHLIAAPVAVLAVGVLTGCPDRSIDKVDPLQGRVEAKDIPVNI
                     NRDIDLLFLIDDSPSMADKQANLAMNFPQFIQVLESIPGGLPNVHIGVATSDMGTQGL
                     LDMNPNFGPTVGAGLPGACSGIGKDGALQVFGQPVNGVFLSDIADAMTGVRTRNYTGD
                     LATVFGMIAKGAGARGCGFEQHLAAAQRALVNPANAGFLRDNAYLAVIIIADEDDCSL
                     AHYSLLGTDTTANAKLGELQSFRCTRFGVVCNQGGQSTDQMNQVGTKSQCHPADDTTY
                     LAKIDDFVTNLKKVKNNDPNKVIVAGILGTVDRFAVELRTPPGEKTAVPALAHSCSYI
                     DRNGGTEVADPPIRTKAFLDQFPNRSTFVPICQNDLSGGLQQIGDLLKTVIGDPCIEG
                     KLQNFGTAASPVYDCSVSSVANPGTASAKETIMAECPATGPLPTTPCWHIVTDNTKCA
                     MTPDHLVLKIENDDALKTPGTDVHVLANCVTQSQ"
     gene            2563..>2925
                     /locus_tag="VF516_43070"
     CDS             2563..>2925
                     /locus_tag="VF516_43070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114611.1"
                     /translation="MRAFSQRLAAGARWMPVAPVVMLLVVTSASVGAVASGSVRHVLL
                     LGADDDARRALHLMLDRFGKQVSAVAELDGARSYLGNGHDCDAVIASGELAAQLVRGG
                     GVPPVIAVVRPRDVTASLA"
BASE COUNT          420 a          930 c         1082 g          493 t
ORIGIN      
        1 agcgggaaca gcaggatcac gccgatgccc tgcatctggg tccaggtctt ggccttggcg
       61 aggtagctgg tcttgagcgt caggtcgcgc tgctcgtagg ccgagcgcag cgcggtgacg
      121 aagaactcgc gctggaacag cagcgcgacc atccaccatg gcaccagcgg ccgggcgacg
      181 tcgacgaatg gcgtgtaggc gaacgcgatg aagatcttgt cggcgatcgg atcgagcagg
      241 ccgccgagca cggtcgggcc gtacttgcgg gcgagcaggc cgtcgatcca gtcggtgcag
      301 ccgatcaccg tcccggcgat cagcgcgctc gtcatgtagg cgttgtgctt gtagccctgc
      361 tcggcgcccc ggtagatcag ccagctgagc agcggcatcg ggatcaggcg tgccagcgtc
      421 acctggttcg cggtgatcag gggacggccg ctcggcacga acgcacccta tcacgcgcag
      481 gcgacagcgg aaggcgctgg accgggccgc cgcggcgact gcgcgccggc cgaccgagct
      541 gagcctacgc gccggcgggg gtcactgtga ctgggtgacg cagttcgcga gcacgtggac
      601 gtcggtgccc ggcgtcttga gcgcgtcgtc gttctcgatc ttgagcacga ggtgatccgg
      661 cgtcatcgcg cacttggtgt tgtcggtgac gatgtgccag cacggcgtgg tcggcagcgg
      721 cccggtggca gggcactccg ccatgatggt ctccttcgcg ctggccgtgc ccgggttggc
      781 caccgacgac accgagcagt cgtacaccgg gctcgccgcc gtgccgaagt tctgcagctt
      841 gccctcgatg cagggatcgc cgatcaccgt cttgagcaga tcgccgatct gctgcagccc
      901 gccggacagg tcgttctggc agatcgggac gaacgtgctg cggttgggga actggtcgag
      961 gaacgccttg gtgcggatcg gcggatcggc gacctcggtc ccgccattgc ggtcgatgta
     1021 cgagcacgag tgcgcgagcg ccgggaccgc ggtcttctcg cccggcggtg tgcgcagctc
     1081 gaccgcgaac cgatccaccg tgccgaggat gccggcgacg atgaccttgt tggggtcgtt
     1141 gttcttgacc ttcttgaggt tggtgacgaa gtcgtcgatc ttggccaggt acgtggtgtc
     1201 gtcggcgggg tggcactggc tcttggtgcc gacctggttc atctggtcgg tgctctggcc
     1261 gccctggttg cagaccacgc cgaaccgcgt gcagcggaac gactgcagct caccgagctt
     1321 ggcgttggcc gtggtgtcgg tcccgagcag cgagtagtgc gccagcgagc agtcgtcctc
     1381 gtcggcgatg atgatgacgg cgaggtaggc gttgtcgcgc aggaagccgg cgttggccgg
     1441 gttgaccagc gcgcgctggg cggccgcgag gtgctgctcg aagccgcagc cccgggcgcc
     1501 ggcgcccttg gcgatcatgc cgaacacggt cgcgaggtcg ccggtgtaat tccgcgtccg
     1561 cacgccggtc atcgcatcgg cgatgtcgct gaggaacacg ccgttgaccg gctgcccgaa
     1621 cacctgcagg gcgccgtcct tgccgatccc cgagcacgcg ccgggcaggc cggcaccgac
     1681 ggtcggaccg aagttcgggt tcatgtccag caggccctgc gtgcccatgt ccgaggtcgc
     1741 gacgccgatg tgcacgttgg gcaggccgcc cgggatcgac tcgagcacct ggatgaactg
     1801 cgggaagttc atggcgaggt tggcctgctt gtcggccatc gacggcgagt catcgatcag
     1861 gaacagcaga tcgatgtcgc ggttgatgtt gaccgggatg tccttggcct cgacgcggcc
     1921 ttgcagcgga tcgaccttgt cgatcgaccg gtcgggacag ccggtcagga cgcctacggc
     1981 cagaacggcg accggagcgg cgattagatg gatcatcgaa catgcaacga cggaccggcg
     2041 catgaggacc tccagggcgg ggacagcata gagcaacttg ctcgccaagt tgagtcacga
     2101 gcagtttcaa atgcttactt ttgttggaag gacacgttca ccgccaacct ggttgccatc
     2161 gcagcgcacc ggttggcgtg tgcgtgcggc ggttttccat ggctccgggc cgagttgacg
     2221 gcgctgcgag gtcgcccacg tggtcgatcg tggggcgatg cacggcgctt gcgcagtggt
     2281 ggatcgcacg cccgtcgcgc accggatcac atcgccgcgg gcggctccgg atcgttggcg
     2341 gcgagcgctg ccagcgagcg cgggtgcggc gatcggcagc cgtgcgccga tcgatcgcac
     2401 ggtgccggac cgttctgcgc gccgatcgcc gggacgcgct gcgctgcgag cagcgtcgat
     2461 gaaccgcaaa gccgcgcgat ccacgtacgc gcgcgcgacg cgcccgccgg gaatgcgctt
     2521 cgcgatgcgg gcgttcttgc agcatgcggg cgttcttgca gcatgcgggc gttctcgcag
     2581 cgcctggccg cgggcgcgcg ctggatgccg gtcgcgcccg tggtaatgtt gctggtcgtg
     2641 accagcgcga gcgtgggtgc cgttgcctcc ggatcggtgc gccacgtcct cttgctcggc
     2701 gccgacgacg atgcgcgccg cgcgctgcac ctgatgctcg accgcttcgg caagcaggtg
     2761 tcggcggtcg ccgagctcga cggggcgcgc agctacctcg gcaacggcca cgactgcgac
     2821 gcggtgatcg cgtcgggcga gctcgccgcg cagctggtgc ggggcggcgg cgtgccgccg
     2881 gtgatcgcgg tggtccggcc gcgggacgtg accgcgtcgc tcgcg
//
LOCUS       DASVOR010002336         3340 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_55457,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002336 DASVOR010000000
VERSION     DASVOR010002336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3340
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55457"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VF516_43075"
     CDS             <1..1124
                     /locus_tag="VF516_43075"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEX8114612.1"
                     /translation="DRRARVIGVAAAALATGVAMLVTHAVRAALGMHAYPLGFGRPGD
                     VARFMLALAVLYAAFTSVKEALLRVALRGHADRAEPEPVLETSALLYVLGGVVGAPMQ
                     LAAYAVYAGDSQLTWVCVMLWSVLVNAVIAREVGRMWQVGALLRELAVKERLAAIGET
                     TARIVHQTRHQLGLIGFIVHRIERRIGGLPEADLAVVRGELAKLGEVQDELRRMLARD
                     LRGEPAGEPVPRATYAALIRRAVARLDGLAAARGVIVEAQQLTAAEPLGPRATEHIAQ
                     ALFNVLENAIVAARGKVQIAACRDGAALVLSIADDGPGLAPAIADTALDPFATTKPDG
                     TGMGLPIARAAIGEEGGALRIGRAPGGGLAVDFVVPVPA"
     gene            1258..2139
                     /locus_tag="VF516_43080"
     CDS             1258..2139
                     /locus_tag="VF516_43080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114613.1"
                     /translation="MVPALAPAVAHAGSCTNGFDPFAPFLPNVLCHSQAPVSVAFSHD
                     GLRSRNVFWAPGAGRGPRPAVVLFQGTSSLGADSLGLDQNGGVLGPGGTWNHAIDTAA
                     PFGGFFQVKVVQALLDAGFTVIQPAAHYQLGIGYFWDTNVGWDGSEDQQLIPALIAAI
                     ESGAFGSVDVAHLYATGISSGGYMTSRMANEFASRPGQLDPARPFRAVAIESASFESC
                     AGALCVIPTPLPATHPPTLFLHGLVDPIVPIATAQAYDAVLKAQAIPERFVVDPLASH
                     QWIAAAPSEVVAWFQGH"
     gene            2257..3201
                     /locus_tag="VF516_43085"
     CDS             2257..3201
                     /locus_tag="VF516_43085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEX8114614.1"
                     /translation="MSSDIRAAALDPARVAKLVVIGIPHPATLKPTPINLWRVRHFVL
                     YKLPGAARRFAADDFAALPAICKRWSPLWSPSTDELAPVRAAFSDPASLDAALGYYRA
                     LRFRPPAFLRRPITVPTIAFAGLDDPNVSPADYAAARRMFQADYAVEEMRGGHFMHRE
                     YPTTSPPSCSRTCDHTRSRARWPPYSTAIEPTKPCAVHMKLKVPALSNSRHAVLPKST
                     ISAGSVGLSGLGATLCAGDITNLTRPCALIVVIIGVYMTSLSLVVTVGMSPGGQVTVR
                     TLGAAASPIAAASGFDEELELLHAARIRQASDGQTRMA"
BASE COUNT          402 a         1281 c         1168 g          489 t
ORIGIN      
        1 ccgaccggcg cgcgcgcgtc atcggggtcg ccgcggcggc gctggccacc ggcgtcgcga
       61 tgctcgtcac ccacgcggtg cgcgccgcgc tcggcatgca cgcctacccg ctcgggttcg
      121 gccgtcccgg cgacgtcgcg cggttcatgc tcgcgctcgc ggtgctctac gccgcgttca
      181 cctcggtcaa ggaggcgctg ctccgggtcg cgctgcgcgg ccacgcggac cgcgccgagc
      241 ccgagccggt gctggagacc agcgcgttgc tgtacgtgct gggtggcgtg gtcggcgcgc
      301 cgatgcagct cgccgcgtac gcggtgtacg ccggcgactc gcagctcacc tgggtgtgcg
      361 tgatgctgtg gagcgtgctc gtcaacgcgg tgatcgcgcg cgaggtcggg cggatgtggc
      421 aggtcggcgc gctgctgcgc gagctcgccg tcaaggagcg gctcgccgcg atcggcgaga
      481 ccaccgcgcg catcgtccac cagacccgcc accagctcgg gctgatcggc ttcatcgtgc
      541 atcgcatcga gcgccggatc ggcgggctcc ccgaggcgga cctcgcggtg gtgcgcggcg
      601 agctcgccaa gctcggcgag gtccaggacg agctgcgccg gatgctcgcc cgcgatctcc
      661 gcggggagcc ggccggcgag ccggttccgc gcgcgaccta cgccgcgctg atccgccgcg
      721 cggtcgcccg gctcgacggg ctcgccgcgg cgcgcggggt catcgtcgaa gcccagcagc
      781 tcaccgcggc cgagcccctg ggcccgcgcg ccaccgagca catcgcgcag gcgctgttca
      841 acgtgctgga gaacgcgatc gtcgcggcgc gcggcaaggt ccagatcgcc gcctgccggg
      901 acggcgccgc cctggtgctg tcgatcgccg acgacggccc cggcctggcg ccggcgatcg
      961 cggacaccgc actggatccg ttcgccacca ccaagcccga cggcaccggc atgggcctgc
     1021 cgatcgcccg cgcggcgatc ggcgaggaag gtggcgcgct gcggatcggc cgcgcgccgg
     1081 gcggcgggct cgcggtggat ttcgtggtcc cggtcccggc gtgacgcact cgctgcgcgg
     1141 cggtcgccgc tctcccttgg tccccccacg gcactgccgt gtccgtccac tcctcctcga
     1201 caggtcaggt gaaccatgca gcgtcttcgc gtcactgtcc tctctctact ggccgcggtg
     1261 gtccccgcgc tggctccggc ggtcgcgcac gccggctcct gcacgaacgg cttcgatccg
     1321 ttcgcgccgt tcctgcccaa cgtcctctgt cactcgcagg cccccgtctc cgtcgcgttc
     1381 agccacgacg gcctgcgatc gcgcaacgtg ttctgggcgc cgggcgccgg ccggggcccg
     1441 cggccggcgg tcgtgctgtt ccagggcacc agctcgctcg gcgccgattc gctgggcctc
     1501 gatcagaatg gcggcgtgct cgggccgggc gggacgtgga accacgcgat cgacaccgcg
     1561 gcgccgttcg gcggcttctt ccaggtcaag gtcgtccagg cgctgctcga cgccggcttc
     1621 acggtgatcc agcccgcggc gcactatcag ctcggcatcg gttacttctg ggacaccaac
     1681 gtcgggtggg acggctccga ggatcagcag ctcatcccgg cgctgatcgc ggcgatcgag
     1741 agcggcgcgt tcgggtcggt cgacgtcgcg cacctctacg ccaccgggat ctcgagcggc
     1801 ggctacatga ccagccgcat ggccaacgag ttcgcgagtc gcccgggcca gctcgatccg
     1861 gcgcgcccgt tccgcgcggt ggcgatcgag tccgcgtcgt tcgagtcctg cgccggggcg
     1921 ctgtgcgtca tcccgacgcc gctcccggcg acccacccgc cgacgctgtt cctccacggc
     1981 ctggtcgatc cgatcgtccc gatcgccacc gcgcaggcct acgacgccgt cctgaaggcg
     2041 caggcgatcc ccgagcggtt cgtggtcgat ccgctcgcga gtcaccagtg gatcgcagcg
     2101 gcgccgagcg aggtcgtcgc ctggttccag ggccactgag ctactggccg cccgccgcgc
     2161 ggtacgtcgc gcgccagcgg ctgaccagcg gcccgtgtgc ggcagatcac ggcgcgcacc
     2221 ggccagccgc tccgcgcccg ggcgcatcct cgcgccatgt ccagcgacat ccgcgccgcc
     2281 gcgctcgacc cggcgcgggt cgccaagctc gtcgtgatcg gcatcccgca cccggccacg
     2341 ctcaagccga cgcccatcaa cctgtggcga gtccgccact tcgtgctcta caagctcccc
     2401 ggcgccgccc ggcgcttcgc cgccgatgac ttcgccgcgc tgcccgcgat ctgcaagcgc
     2461 tggtcgccgc tgtggtcgcc gtcgaccgac gagctcgccc cggtgcgcgc cgcgttctcc
     2521 gaccccgcca gcctcgacgc ggccctgggc tactaccgcg cgctccggtt tcgccccccg
     2581 gcgttcctgc gccggccgat cacggtcccc accatcgcgt tcgccggcct cgacgacccc
     2641 aacgtcagcc ccgccgacta cgccgccgcc cgccggatgt tccaggccga ctacgcggtc
     2701 gaggagatgc gcggcggcca cttcatgcac cgcgagtacc cgacgacttc gccgccaagc
     2761 tgctcgcgca cctgtgatca cacgcgctcg cgagcgcggt ggccgcccta ctccacggcg
     2821 atcgagccga cgaagccgtg cgccgtgcac atgaagttga aggtgccggc cttgtcgaac
     2881 tccaggcacg cggtcctgcc gaagtcgacc atcagcgccg gatcggtcgg cttgagcggg
     2941 ttgggcgcca cgttgtgcgc cggtgacatc acgaacttga cgaggccgtg tgcgctgatc
     3001 gtcgtcatca tcggcgtgta catgacgtca ctgtcgctgg tcgtgaccgt cggcatgtcg
     3061 cccggcgggc aggtcaccgt ccgcacgctc ggcgccgcgg cgtcgcccat cgccgcggcg
     3121 tcggggttcg acgaggagct ggagctgctg cacgcggcga ggatcaggca agcaagcgat
     3181 ggacagactc gcatggcgtg accacgacga tacccgcggc gcagaagctt cgccaaaccg
     3241 ctacggcagc tgtgcgggat ccgtcacggg gccggtgtgc gacggatccg cagccggtgc
     3301 cgcgcgcggc tcggcgtcgc gcgccgccgg aggggacgat
//
LOCUS       DASVOR010002337         1914 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_346301,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002337 DASVOR010000000
VERSION     DASVOR010002337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346301"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VF516_43090"
     CDS             complement(<1..683)
                     /locus_tag="VF516_43090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEX8114615.1"
                     /translation="MRSNVKIGNKLVGDGRPVYVIAEIGINHNGSVDIAKRMIDGAVL
                     AGCDAVKFQKRTPEACVPRDQWLIERDTPWGRMTYIDYRHRIEFGAAEYAAIDAYCRD
                     RGIQWFASAWDEPSVEFLEQFEPPCYKAASASLTDHALLRAMKATGRPLIISTGMSTM
                     DEIEAAVEAIGRDQLLIAHSTSSYPCPVEAINLNMIATLRRRYEERPIGYSGHEVGLS
                     PTWAAVALGA"
     gene            complement(680..1213)
                     /locus_tag="VF516_43095"
     CDS             complement(680..1213)
                     /locus_tag="VF516_43095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonate 8-phosphate
                     phosphatase"
                     /protein_id="HEX8114616.1"
                     /translation="MVTPRCPADAARRAIRLALVLTDNDGVLTDGTVHVGPSGEQMKV
                     YSVRDGMGVERLRERGIAAGVLTREPPELIAPRAAKLGMKLWAGVRDKHAQLGRILDQ
                     AGVGLDQIAYIGDDVNDLGVIEAVRDVGLTAAPADAMPAVRDAVHYICEAPGGRGAFR
                     EVAEWLLRLRTTPEGSS"
     gene            complement(1207..>1914)
                     /locus_tag="VF516_43100"
     CDS             complement(1207..>1914)
                     /locus_tag="VF516_43100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114617.1"
                     /translation="GRVVITDLDHAACVDRAAALGDAGIGCGADITRRDSLAALLAAV
                     LDRFGQIDVLVNSAAIDDKFGGSAAHDSQFEHYALERWQRMLDANVTGTFLACQVLGA
                     AMLRGAPRDRSIINIASTYGIVAPDQKLYRRPDGSQPFFKSPAYPTAKAAVLGFTRYL
                     AAYWGAAGIRVNALSPGGVENGQPDHFVAGYCARTPLGRMARPDDYAGATVFLAGAAS
                     SYMTGANLVVDGGFTAW"
BASE COUNT          263 a          661 c          714 g          276 t
ORIGIN      
        1 gcgcccagcg cgaccgcggc ccaggtcggc gacaggccga cctcgtggcc ggagtagccg
       61 atcggccgtt cctcgtagcg ccggcgcagc gtggcgatca tgttgaggtt gatcgcctcg
      121 accgggcacg gatacgacga ggtcgagtgc gcgatcagga gctggtcgcg gccgatcgcc
      181 tcgaccgcgg cctcgatctc gtccatcgtc gacatcccgg tcgagatgat cagcggccgg
      241 ccggtcgcct tcatcgcgcg gagcagcgcg tggtcggtga gcgaggccga cgcggccttg
      301 tagcacggcg gctcgaactg ctcgaggaac tcgaccgacg gctcgtccca cgccgaggcg
      361 aaccactgga tgccgcggtc gcggcagtag gcgtcgatcg cggcgtactc ggcggcgccg
      421 aactcgatcc tgtggcggta gtcgatgtag gtcatccggc cccagggcgt gtcgcgctcg
      481 atcagccact gatcgcgcgg cacgcacgcc tcgggggtgc gcttctggaa cttgacggcg
      541 tcgcaacccg cgagcaccgc gccatcgatc atgcgcttgg caatgtcgac cgagccgttg
      601 tggttgatcc cgatctccgc gatcacgtag accgggcggc cgtcgcccac cagcttgtta
      661 ccgatcttga cgttgctcct catgaggaac cctccggtgt ggtgcgcagg cgaagcagcc
      721 actccgcgac ctcgcggaat gcgccgcgcc cgccgggtgc ttcgcagatg taatggaccg
      781 cgtcgcgcac ggccggcatc gcgtcggccg gcgcggcggt caggccgacg tcgcggacgg
      841 cctcgatcac gccgaggtcg ttgacgtcgt cgccgatgta cgcgatctgg tcgagcccga
      901 cgccggcctg atcgaggatc cggccgagct gagcgtgctt gtcgcgcacg ccggcccaca
      961 gcttcatgcc gagcttggcg gcgcgcggcg cgatcagctc gggcggctcg cgggtcagca
     1021 cgccggcggc gatgccgcgc tcgcgcagcc gctcgacgcc catgccgtcg cgcaccgagt
     1081 agaccttcat ctgctcgccg gatggcccga cgtggaccgt gccgtcggtc agcacgccgt
     1141 cgttgtcggt cagcaccagg gcgagccgga tcgcgcggcg cgcggcgtcg gcggggcagc
     1201 gcggggtcac cacgcggtga accctccgtc gacgaccagg ttggcgccgg tcatgtacga
     1261 cgacgcggcg ccggcgagga acacggtggc gccggcgtag tcgtcgggcc tggccatccg
     1321 gcccagcggc gtgcgcgcgc agtagcccgc gacgaagtgg tcgggctggc cgttctcgac
     1381 cccgccgggc gacagcgcgt tgacccggat ccccgcggcg ccccagtagg cggcgaggta
     1441 gcgggtgaag ccgagcaccg cggccttggc ggtcgggtag gccggggact tgaagaacgg
     1501 ctggctgccg tcggggcggc ggtacagctt ctgatcgggc gcgacgatgc cgtaggtcga
     1561 cgcgatgttg atgatcgagc gatcgcgggg cgcgccgcgc agcatcgccg cgccgagcac
     1621 ctggcacgcc aggaacgtgc cggtgacgtt ggcgtcgagc atgcgctgcc agcgctcgag
     1681 cgcgtagtgc tcgaactgcg agtcgtgcgc cgccgagccg ccgaacttgt cgtcgatcgc
     1741 ggcgctgttg accagcacgt cgatctggcc gaaccgatcg agcacggcgg cgagcagcgc
     1801 ggcgagcgag tcgcgccggg tgatgtcggc gccgcacccg atcccggcgt cgcccagggc
     1861 ggcggcgcgg tcgacgcacg cggcgtggtc gagatcggtg atcacgaccc ggcc
//
LOCUS       DASVOR010002338         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_259912,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002338 DASVOR010000000
VERSION     DASVOR010002338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259912"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VF516_43105"
     CDS             <1..864
                     /locus_tag="VF516_43105"
                     /inference="COORDINATES: protein motif:HMM:NF016150.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEX8114618.1"
                     /translation="LLLSWVDVTWGYALVGLFLLPFVRASDRTRLIAAAALTILPALV
                     YAIPQVAPWFHALVFDQPRAAYIDQFAAAARSGDRIAILHAHVAIALLWTFGSTGVWY
                     LPFMLARFLLGLVAGARRWFDHDGADHVAVFRKVLIAGALGTVVGWVPEALAAAHVFD
                     PAGHGIPVTMALTVVSRLALLAQVAMYVAGIVLLMQRPAWRRVLVVIAPVGRMPLTTY
                     LMQSLICTSLFYGWGLAWTTPPPAACVGLGLAIFAVQIVIAHAWLRWFRFGPAEWLWR
                     SIVYWRLQPMR"
     gene            complement(878..>1835)
                     /locus_tag="VF516_43110"
     CDS             complement(878..>1835)
                     /locus_tag="VF516_43110"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8114619.1"
                     /translation="GTIVELDRVTKSLGGKLLFRDLTLVMKPGDRIGVVGPNGAGKTT
                     LIRTILGLLPPDAGSVVLGANTRPAYLEQGRSELRDEQTVLDEVGDGYDFVELPDGKI
                     HVRSFLRMLAFSDSVADTKVGQLSGGERNRVQLARLLRRGGNLLVLDEPTNDLDLPTL
                     GALEDGLVHFAGCALIVSHDRWFLDRVATAILAFEGDGRVTLYEGSYTFYAERRPKPA
                     ERTDRPRRDSRDERAAARPTGPRKLTFKEARELEAIEDTIAAAEARVAELETQLSDPG
                     VFKDRPTEVPALVAGLDAARAEVERLFARWQELDAIHRASPR"
BASE COUNT          202 a          652 c          689 g          292 t
ORIGIN      
        1 ctgctgctgt cctgggtcga cgtgacgtgg gggtatgcgc tggtcggcct gttcctgctc
       61 ccgttcgtgc gcgccagcga tcgcacccgg ctgatcgccg cggcggcgct cacgatcctc
      121 ccggcgctgg tctacgcgat cccccaggtc gcgccgtggt tccacgcgct ggtgttcgat
      181 cagccgcgcg ccgcctacat cgaccagttc gccgccgcgg cgcggagcgg cgaccggatc
      241 gcgatcctgc acgcgcacgt ggcgatcgcg ctgctatgga cgttcggcag caccggggtg
      301 tggtacctgc cgttcatgct cgcccggttc ctgctcgggc tggtcgccgg cgcccggcgc
      361 tggttcgacc acgacggcgc cgaccacgtc gcggtgttcc gcaaggtgct catcgccggc
      421 gcgctcggga cggtggtcgg ctgggtgccc gaggcgctcg ccgcggcgca cgtgttcgac
      481 ccggccggcc acgggatccc ggtgacgatg gccctgaccg tcgtgtcgcg gctggcgctg
      541 ctcgcgcagg tcgcgatgta cgtcgcgggc atcgtgctgc tgatgcagcg acccgcgtgg
      601 cgccgcgtgc tcgtcgtgat cgcgccggtc ggccgcatgc cgctgaccac gtacctgatg
      661 cagagcctga tctgcacgtc gctgttctac ggctgggggc tggcctggac cacgccacca
      721 ccggcggcgt gcgtcggcct cgggctcgcg atcttcgcgg tccagatcgt gatcgcgcac
      781 gcctggctgc gctggttccg gttcggcccc gccgagtggc tgtggcgatc gatcgtctac
      841 tggcgcctcc agccgatgcg gtgacgccgg gcgccggtca ccgcgggctc gccctgtgga
      901 tcgcgtcgag ctcctgccag cgcgcgaaca gccgctcgac ctcggcgcgc gcggcgtcga
      961 gcccggcgac cagcgccggg acctcggtcg gccggtcctt gaacacgccc ggatccgaca
     1021 gctgggtctc gagctcggcg acgcgggcct cggcggcggc gatcgtgtcc tcgatcgcct
     1081 cgagctcgcg ggcctccttg aacgtgagct tgcgcgggcc ggtgggccgg gcggcggcgc
     1141 gctcgtcgcg ggaatcgcgg cgcgggcggt cggtgcgctc ggccggcttc ggccggcgct
     1201 cggcgtagaa cgtgtagctg ccctcgtaca gcgtcacccg gccgtcgccc tcgaacgcga
     1261 ggatcgcggt ggcgacccgg tcgaggaacc agcggtcgtg cgacacgatc agcgcgcagc
     1321 cggcgaagtg gaccaggccg tcctcgagcg cgcccagggt cggcaggtcg aggtcgttgg
     1381 tcggctcgtc gagcacgagc aggttgccgc cgcggcggag caggcgcgcg agctggaccc
     1441 ggttgcgctc gccgcccgac agctggccga ccttggtgtc ggcgaccgag tcggagaacg
     1501 cgagcatgcg caagaagctg cggacatgga tcttgccgtc gggcagctcg acgaagtcgt
     1561 agccgtcgcc gacctcgtcg agcacggtct gctcgtcgcg cagctcgctg cggccctgct
     1621 cgaggtacgc cggccgggtg ttggcgccga gcacgacgct gccggcgtcg ggcgggagca
     1681 gcccgaggat cgtgcggatc agcgtggtct tgccggcgcc gttggggccg accacgccga
     1741 tccggtcgcc cggcttcatg accagcgtca ggtcgcggaa caggagcttg ccgcccagcg
     1801 acttggtgac ccggtcgagc tcgacgatcg tgccg
//
LOCUS       DASVOR010002339         2193 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_259942,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002339 DASVOR010000000
VERSION     DASVOR010002339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259942"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VF516_43115"
     CDS             complement(<1..925)
                     /locus_tag="VF516_43115"
                     /inference="COORDINATES: protein motif:HMM:NF014698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HEX8114620.1"
                     /translation="MTDNLDTAWPLVIGPDEPQAVLEDWVAANRDRLGSQLIECKAIL
                     FRGFRARGGFESIANSFFDRRLNYTYRSTPRTDVGQNLYTATEYPKQLSIPQHCENAY
                     QRDWPMRLLLYCAEPASKGGRTPLADMTKVTAMIAPEIREQFARKRVRYVRNYRTGVD
                     LPWQEVFGTTSKAEVEKFCVEHDMEYQWTESGLRTIQVCQALATHPISGEQVWFNQAH
                     LFHLSALDAASQKMLLSYFGEAGLPRNAYFGDGTPIAVDVLDQIRAAHDRNKVSFEWQ
                     RGDVLLIDNMLVCHGRDPFDGSRRVLVCMAEP"
     gene            complement(1054..>2193)
                     /locus_tag="VF516_43120"
     CDS             complement(1054..>2193)
                     /locus_tag="VF516_43120"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEX8114621.1"
                     /translation="SRYYKPELQTISLGLLSDDGDRPFVLSTPRLPDAKHLHVKVDFR
                     SEFIDALARARETPLDGAGVTRLFEGLETAGGLPRDVLFTERKPVHAYVPVASGVRLR
                     YTGHAGFLFETPDVAIAVDPVIANRCHKYADQMISFSELLPLINYVCLTHNHQDHVHL
                     ETLLQLRHKIGKLVVPKNNGGTIVDPSLKLMLQQLGFQVIEVDELEAIEFPGGRIVSV
                     PFLGEHGDLHVRSKNAWLVEAAGKRVFFGADSSNPDIRMYENMGGLMREIDVFCIGME
                     CVGAPYTWLYGALHTRTVNKAIMNSRRLNGSDSQQAFDIVNLVRPQHVCIYALGMEPW
                     YKYFMGIDYSGNSKQINESRRMIDLCAGIGIGAESLYGKKTIEMT"
BASE COUNT          408 a          698 c          674 g          413 t
ORIGIN      
        1 aaggctccgc catgcagacc agcacccgcc ggctgccgtc gaacggatct cggccgtgac
       61 agaccagcat gttgtcgatc agcaacacgt cgcctcgctg ccactcgaac gagaccttgt
      121 tgcggtcgtg cgccgcacgg atctggtcga gaacgtccac cgcgatcggc gttccgtcac
      181 cgaagtaggc gttgcgcgga agcccggcct cgccgaagta gctgagcagc atcttttgcg
      241 acgccgcatc cagcgcggac agatgaaaca ggtgcgcctg attgaaccag acctgctcgc
      301 cgctgatcgg gtgggtcgcc agcgcctggc agacctggat cgttctcagg ccgctctcgg
      361 tccactggta ctccatgtca tgctcgacgc agaacttctc gacctcggcc ttgctggtgg
      421 ttccaaacac ctcctgccac ggcaggtcga cgccggtgcg gtagttgcgg acgtagcgga
      481 cccgcttgcg cgcgaactgc tcccggatct ccggcgcgat catcgcggtc accttcgtca
      541 tgtcggccag cggcgttcga ccgcccttgc tcgccggctc tgcacaataa agcaacagcc
      601 gcatcggcca gtcacgctga taagcgttct cgcagtgctg cgggatcgac agctgcttgg
      661 gatactcggt cgccgtgtac aggttctggc cgacgtcggt gcgcggcgtc gagcggtagg
      721 tataattgag ccgccggtcg aagaagctgt tggcgatgct ctcgaagcca ccccgggcgc
      781 gaaagccgcg aaacaggatc gccttgcatt caatcagctg cgatccgagc cggtcgcggt
      841 tcgccgcgac ccagtcctcg agcacagctt gcggctcgtc ggggccaatg accaggggcc
      901 acgcagtatc caggttgtcg gtcatcacca tcctccgggg ctcagatgta cactccgtga
      961 cacagctcgt gcagcaggcg cgatgtgatc tgacaccggg gcagagccca gttgcttagg
     1021 tcctgctcat cgcaccgttc aagtcgggtc ggatcaggtc atctcgatcg ttttcttgcc
     1081 gtacagcgac tcggcgccga tgccgatgcc ggcacacaag tcgatcatcc gacgcgattc
     1141 gttgatctgt ttggaatttc cactgtagtc gatgcccatg aagtacttgt accaaggctc
     1201 catgccgagg gcgtagatgc acacgtgctg cgggcgcacc aggttgacga tatcgaacgc
     1261 ctgttgcgag tcggagccgt tcaaccgtcg cgagttcatg atcgccttgt tgacggtgcg
     1321 agtatgcaac gcgccgtaca gccaggtata gggggcgccg acacactcca tgccaatgca
     1381 aaacacatcg atttcgcgca tcagcccacc catgttctcg tacatgcgga tgtcggggtt
     1441 tgacgaatcc gcgccaaaga acacgcgctt gcccgccgcc tcaaccagcc aggcgttctt
     1501 cgagcgcacg tgcaggtcgc cgtgctcgcc caagaacggc acgctgacga tgcgcccgcc
     1561 gggaaactca atggcctcca gctcgtcaac ctcgatcacc tgaaaaccca gctgctgcag
     1621 catcagcttc agcgaaggat cgacgatggt cccgccgtta ttcttcggca ccaccagctt
     1681 gccaatctta tggcgcaact gcagcagggt ctcgaggtgg acgtggtctt ggtggttgtg
     1741 ggttaggcac acatagttga tgagcggtag tagttcgctg aagctgatca tctggtcggc
     1801 gtacttgtgg caacggttcg cgataactgg gtctaccgcg atagccacgt cgggcgtctc
     1861 gaacaggaaa ccggcgtggc cagtgtagcg caggcgcacg ccggacgcca ccggcacgta
     1921 ggcgtggact ggcttgcgct cggtgaacag cacatcgcgg ggcaacccgc ccgccgtctc
     1981 caagccctcg aacaggcgcg tgaccccggc cccgtccagc ggcgtctcgc gcgcccgggc
     2041 cagggcatcg ataaactccg agcgaaaatc caccttgacg tgcaggtgct tcgcgtcggg
     2101 cagacgggga gtgctgagaa cgaacggccg gtcgccatcg tccgaaagca ggccgagcga
     2161 gatggtctgc agttcgggtt tgtagtagcg gct
//
LOCUS       DASVOR010002340         1637 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_27151,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002340 DASVOR010000000
VERSION     DASVOR010002340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27151"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1330)
                     /locus_tag="VF516_43125"
     CDS             complement(<1..1330)
                     /locus_tag="VF516_43125"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEX8114622.1"
                     /translation="MTGLSEFEALERRRTDGPNELPAQERQTFSHALVDVIREPMTAL
                     LLACGAIYFFLGDSHEAAMLLGFVAFIVVITLYQERRTERAIAALRDLASPRALVIRD
                     GRRQRIAGRDVVRDDLVVLAEGDRVPADGIVRSTAHCTVDESLLTGEAMAVAKREWHG
                     EALEGRPGGDDQPLVYAGTLVVQGIAVAQVTATGERSEIGRIGRSLSATATESTRLQL
                     ETRGIVWKLALLGGALSLAVALTYAVQRHDLIAGVLAGLTLAMAILPNEFPVVVTLFL
                     ALGAWRLSRRRVLTRRIHAVEALGSVTVMCVDKTGTLTENRMTVSRLAVEGLAFDVAR
                     LAHEPLPEDVHALVEYAVLASRKDPFDPMELAFARLASEQLAGTEHLHPDWTLVREYP
                     LSRERLAVLQIWRPPDGELVVACKGAPEAVARLCRLDARQADQAAARARAM"
     gene            complement(1445..>1637)
                     /locus_tag="VF516_43130"
     CDS             complement(1445..>1637)
                     /locus_tag="VF516_43130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114623.1"
                     /translation="SAKGPATAYLLDAIRTHGRRDAVVAARALATFKDDASLIERIRT
                     AAREQRDPAARDEIAELLA"
BASE COUNT          237 a          587 c          595 g          218 t
ORIGIN      
        1 ccatcgcacg ggcgcgcgcc gcagcctggt cggcctgtcg tgcgtcgagc cggcacagcc
       61 gcgccaccgc ctcgggcgcg cccttgcagg cgacgacgag ctcgccgtcc ggcggccgcc
      121 agatctggag gaccgccagg cgctcgcgcg acagcgggta ctcgcgaacc agcgtccagt
      181 cgggatgcag gtgctcggtg ccggcgagct gctcggaggc gagccgcgcg aacgcgagct
      241 ccatcggatc gaacgggtcc ttgcggctcg cgagcacggc gtattcgacc agggcgtgca
      301 cgtcctcggg cagcggctcg tgcgcgagcc gcgcgacgtc gaatgcgagt ccctcgaccg
      361 cgagacggga caccgtcatc cggttctcgg tgagcgtgcc ggtcttgtcg acgcacatca
      421 ccgtcaccga gccgagcgcc tcgaccgcgt ggatgcgccg cgtgagcacg cgccgcctcg
      481 acagtcgcca tgcgccgagc gccaggaaca gggtgaccac gacgggaaac tcgttgggca
      541 ggatcgccat cgcgagcgtg aggccggcga gcacgcccgc gatcagatcg tggcgctgga
      601 ccgcgtacgt cagcgcgacg gcgagcgaca gggcgccgcc gagcaacgcg agcttccaga
      661 cgatcccccg ggtctcgagc tgcaggcgcg tgctctcggt ggcggtcgcg ctcagggatc
      721 ggccgatccg tccgatctcg gaccgctccc cggtcgcggt gacctgcgcg acggcgatgc
      781 cctggaccac cagcgtgccg gcgtagacca gcggctggtc gtcgccgccc ggccggccct
      841 ccagcgcttc gccgtgccac tcgcgcttgg caaccgccat cgcttcgccg gtcagcaacg
      901 actcgtcgac ggtgcaatgc gcggtcgacc ggacgatgcc gtcggccggg acgcggtcac
      961 cttcggcgag gaccacgagg tcgtcgcgca cgacgtcgcg accggcgatg cgctgccggc
     1021 gcccgtcgcg gatcacgagc gcgcgcgggc ttgcgagatc gcgcagcgct gcgatcgcgc
     1081 gctcggtcct gcgctcctgg tacagcgtga tcacgacgat gaacgcgacg aagccgagca
     1141 acatcgccgc ttcatgggag tcacccagga agaagtagat cgctccgcat gcgagcagca
     1201 gcgcggtcat cggctcgcgg atcacgtcga cgagcgcatg ggagaacgtc tgacgctcct
     1261 gcgccggcag ctcgttcggg ccgtcggtgc gacgccgctc gagcgcctcg aattcggaga
     1321 gtccggtcaa cgaatcgaca gcggtcaccc tcggggaagt tgcacgccgc ttgccaggat
     1381 ggacgggggc cgatcgcgca cgacttgcgc gtcgcggtcc aagcgccgca gctccagcgc
     1441 cgggtcacgc gagcagctcg gcgatctcgt cgcgcgcggc cgggtcgcgc tgctcgcggg
     1501 cggcggtgcg gatccgctcg atcagcgagg cgtcgtcctt gaacgtcgcg agcgcgcggg
     1561 ccgcgacgac cgcgtcgcgc cggccgtgcg tgcggatcgc atcgagcagg taggcggtgg
     1621 ccggcccctt agcactc
//
LOCUS       DASVOR010002341         3012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_287423,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002341 DASVOR010000000
VERSION     DASVOR010002341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287423"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="VF516_43135"
     CDS             <1..237
                     /locus_tag="VF516_43135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114624.1"
                     /translation="AGTRDHNTETTNQLAAFAQQIAQRDPAVDTDTFDRFWYVLNASF
                     WYGLADVARASRLDYGVLDMAEECSTAFFATDPE"
     gene            complement(443..2269)
                     /locus_tag="VF516_43140"
     CDS             complement(443..2269)
                     /locus_tag="VF516_43140"
                     /inference="COORDINATES: protein motif:HMM:NF014741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HEX8114625.1"
                     /translation="MENELEQLGERIAEQAAHLDAATHRLLADLRAFDTRGGWHVQGA
                     ASCAHWLAWRVGWDLVTARDHVRVARKLAGFAAIDDALRCGEVSYSKVRAMLRVATSD
                     NEVMLLEHAKLMTASQLEKLCRKYALVLRHSQDPHPQGDEQRRYVRRRDTEDGMVKIE
                     AVLHPEEAEVVWAMLDHAAAQLARERDHQPALPGRDSAESGAALRPHANLTGPVSAPA
                     TRDGLEDEACVIGDGLPRPPNTSSTDDSAESFEAASPVARWDAGLDDAASAERCAVGL
                     GGGASGAVHAVVSVGVVEDPDAVAASVASSQPFSDAGPRSGLDRLLDEVEAFRGADEL
                     AHDAFSDLCDAECELGAAEAGQRSSEAISGRREASSLRDRAAAARKAFNRAVALVAMA
                     QGYLRGDRPERAPIEVVLTTPASSLRAGATLDPVEVGEMGESFLSSETARRLSCDAGV
                     VDVVEDEHGTPLSVGRRRRTISGALKRALHKRDGMCGYPGCTNRLFLEGHHIQHWADG
                     GETSLSNAVLLCSFHHRYVHEYGYSIELGPDQRPRFRDPHGRWVPAVPELPCVAELGW
                     PQIRAANAALQVDADTIAGPWDGTPVDYGRIVGHLCAVDGLA"
BASE COUNT          518 a         1069 c          937 g          488 t
ORIGIN      
        1 gccggcaccc gcgatcacaa caccgagacc accaaccagc tcgcggcgtt cgcccagcag
       61 atcgcgcagc gcgatcccgc ggtcgacacc gacaccttcg accggttctg gtacgtgctc
      121 aacgcgagct tctggtacgg cctggccgac gtcgcgcgcg cgtcgcggct cgactacggc
      181 gtgctcgaca tggccgagga gtgctcgacc gcgttcttcg cgaccgaccc cgagtgatca
      241 acagggcgtc ggacacctgc gcgcaccggc gccgccgcgg atcggcacgc ccagggtggg
      301 cggtcgccgt catcgccgtt gtcccgggtg tcgacgggcg gcgaccctgg actgcgggtc
      361 gagatgcccg acgatgcgcg gcgcggcacg agccgctcac gcgacttctt gcgagtgggc
      421 cacgtcgggg ttccgtggat cgtcatgcca ggccatcgac ggcgcagaga tgccccacga
      481 tccgcccgta gtccaccggc gtgccgtccc atggcccggc gatcgtatcg gcgtcgacct
      541 gcagcgccgc gttcgccgcg cggatctgcg gccaccccag ctccgccacg catggcaact
      601 ccggcaccgc cgggacccat cgaccatgcg gatcgcggaa tcgcggccgc tgatcgggcc
      661 cgagctcgat cgaatatccg tactcgtgaa cataccggtg gtggaatgag cagagcaata
      721 cggcattcga aaggctggtc tcgccaccgt ctgcccagtg ctggatgtgg tggccttcca
      781 ggaagaggcg gttcgtgcac ccgggatagc cacacattcc gtcgcgcttg tgcagcgcgc
      841 gcttgagcgc cccgctgatc gtgcggcgcc tgcggccgac cgacagcggc gtgccatgct
      901 catcctcgac cacatcgacg acgcccgcgt cgcagctcag ccggcgcgcc gtctcgctgg
      961 acaggaatga ctcccccatc tcaccgacct cgacaggatc cagcgtcgca cccgcgcgaa
     1021 ggctgctcgc cggagtcgtg agcacgacct cgatcggggc acgctccggc cggtcgccgc
     1081 gcagatagcc ctgggccatc gccaccaggg caacggcgcg gttgaaggcc ttccgggccg
     1141 cggcggctcg atcccgcagc gagcttgcct cgcgccggcc gctgatcgcc tccgacgagc
     1201 gctggcccgc ctcagcggct ccaagctcgc actccgcgtc gcacagatcg gagaacgcgt
     1261 cgtgggccag ctcatcggcc ccgcgaaacg cctcgacctc atccagcagt cgatcgaggc
     1321 ccgatcgcgg cccggcatcc gagaacggct gcgaggatgc gacgctcgcc gcgaccgcgt
     1381 ccggatcctc gacgacgccg accgacacga ccgcgtgcac cgcaccggac gcgccgcccc
     1441 ccagccccac tgcacaccgc tccgcggacg ccgcatcatc gagcccggca tcccaccgtg
     1501 cgacagggct cgctgcttcg aacgattccg cagaatcatc ggtagaggag gtgttgggtg
     1561 gccggggcag gccgtccccg atcacgcaag cctcgtcctc caagccgtcc cgcgtagcag
     1621 gcgcagacac cgggcccgtg aggtttgcgt gcgggcgaag tgcagcaccg gattccgcgg
     1681 aatcgcgccc gggtagtgcc ggttggtgat ctcgctcacg cgccagctgc gccgcggcat
     1741 ggtcgagcat cgcccagacc acctcggcct cttccgggtg cagcacggcc tcgatcttga
     1801 ccatgccatc ctcggtatcg cgccggcgca catagcgccg ctgttcgtcg ccctgtggat
     1861 gcgggtcctg gctgtggcgc agcaccagcg cgtacttgcg gcacagcttc tccagctgag
     1921 atgccgtcat cagcttcgcg tgctcgagga gcataacctc gttgtccgag gtggcgacgc
     1981 gcagcatggc gcgcaccttg gagtacgaca cctcgccgca ccgtaacgca tcgtcgatcg
     2041 ccgcaaaccc agcaagcttc cgcgcgacgc gcacatggtc gcgcgccgtg accagatccc
     2101 acccgacgcg ccatgcgagc caatgggcac acgaagcggc gccctgcaca tgccaaccac
     2161 cgcgggtatc gaacgctcgc aggtcggcca gcagccggtg ggtggctgca tcgaggtgtg
     2221 ccgcctgctc ggcgatccgt tcgcccagtt gttcgagctc attctccatg acaaccgcat
     2281 atcatccggg tctgacatcg aacctgcact gcatggcaca ttgatcgctc atcgaccagt
     2341 ttggccttct gtagcacaac aaacaattat ggtctcgcaa ttgctgttat tgtaatcaaa
     2401 cttgcagatc aacatcgcgc tgagcgctca tcagcggtgg tgtcgatcgt ggctgcaccg
     2461 tgggcggtgt cgcgatcatc cgcccgtcaa ggcatggatc tcagcccacg cgattctgtg
     2521 tagataccac tccgacggtc cacgagctca tcagccgtcg cgttcggtcg gccgcagagc
     2581 gcacagaccg ctcgctggat cagccggccg ctcgacggcg atcgagacag cgcatcgccc
     2641 gcagtcgttg cggatggtcc tcgaccgcaa gctctgcaga gcatgtggac ggtcctgtgg
     2701 acaacgggaa ggtctgatga cacgcgccgc agggcgccga ggtcgacctg gacgcgtctc
     2761 acagcccttc ttcagttgcc cacagtcctg acactcgagc cgacgctcca atcagagttg
     2821 aatttttgat ttgttctgga ttcagaccag gagagcgatt gttccaggac acgaagatga
     2881 ctgttcaaca caaggatcaa tgaacaatcc aatgtgattg acttcctgcg ctgtccatcg
     2941 catgaagaaa cgttccctac gtgggaacga ggcagcgccg ccgttggcgc gaggcacgac
     3001 tgacatagcg ga
//
LOCUS       DASVOR010002342         4603 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_114359,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002342 DASVOR010000000
VERSION     DASVOR010002342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114359"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VF516_43145"
     CDS             complement(<1..643)
                     /locus_tag="VF516_43145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114626.1"
                     /translation="MDLKDKIIESREAAHAELQQREERQRKDGKDSLPTPVVEAIHEA
                     GREQRTQLLGAIRMILGRERPDIEQLRMPARETRALQALQAAVEGKSTDTYQFVYASD
                     RRDLLERALAVLQPDLTQTDDKTARELHAQLADLSERLGELRHRLSSLEDAQDELMRS
                     HEAASEIVAAPGDEPEPRPPAKPSDPDAPRPATTLTGPALPEDKPAPSTLTGPA"
     gene            complement(676..903)
                     /locus_tag="VF516_43150"
     CDS             complement(676..903)
                     /locus_tag="VF516_43150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114627.1"
                     /translation="MPKPEPKEIDEKLALQLRHLAEDAVLKGQPYGDERCDNCMYYLH
                     RDDNFSYCWHPKLRILVGGSWWCQWWEKTED"
     gene            complement(1036..2073)
                     /locus_tag="VF516_43155"
     CDS             complement(1036..2073)
                     /locus_tag="VF516_43155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114628.1"
                     /translation="MKLTRYEDVCEELVNSPRRWLVTGVAGFIGSALLERLLDLGQSV
                     VGVDNFLTGHKRNLDDVLAINPDERLQFRFIEGDLRDPAVAREACKDVDIVLHEAALG
                     SVPRSIKDPLASHAHNVDAFLNVLWAAKDAGVGRLVYASSSSVYGDHPGLPKHEDRIG
                     KPLSPYAATKRADEIYAQVFHDVYGQQLIGLRYFNVFGRRQDPDGPYAAVIPRWVANL
                     VAGKPCVIFGDGANSRDFCYVDNIVQANLLAATVPDPAVTGTVYNCGCNGRTDLKQLF
                     VMIRDNLAKTMPEVAGAEPVFEAPRAGDVAHSQAAIDKISAALGYVPSHMVEDGMAET
                     VAWFAHRLRRG"
     gene            complement(2070..3353)
                     /locus_tag="VF516_43160"
     CDS             complement(2070..3353)
                     /locus_tag="VF516_43160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEX8114629.1"
                     /translation="MEHEKIAVIGLGYVGLPVAIAFGKKLSTVGFDIRQRRVDQLKQG
                     HDDTLEVADDQLASARLLEMTADPTRLADCTFYIVAVPTPIDGNNRPDLGPVISASKT
                     IAPHLRPGDVVVYESTVYPGVTEEVCGPLLDQGSGLKNGTDYFLGYSPERINPGDKEH
                     TFDKILKVVSGQTPEILDRVARVYGSVVTAGVHKAPSIKVAEAAKVIENTQRDLNIAL
                     MNELALIFDRMGIRTADVLEAAGTKWNFLKFAPGLVGGHCIGVDPYYLTTKAEELGYL
                     PEVILAGRRINNNVGPFLAQKCVKMLTHSDVALRKARVGILGLTFKENVPDLRNSKIP
                     DIVTELATFGIQAMVHDPLGNPEEAYHEYKIRLTPLEQLVNLDAVILAVAHKAYLGNA
                     GAIYERVRDNGVVIDVKSVLPAQVKPPRGIQLWSL"
     gene            complement(3532..4320)
                     /locus_tag="VF516_43165"
     CDS             complement(3532..4320)
                     /locus_tag="VF516_43165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114630.1"
                     /translation="MSIVRVRVRFLVGLSVAVIAAIWFRAPIPAMSQIALAVAAVAAA
                     RDALIWWRLRRRPPRTAAVVRPGSKPDDAVLASLGIEPGGGELRAACTRVARRIHNEG
                     VRVVGFVPAEDTVAVPPALIQLGIALTELTGATIAVVDANVRYPGLGMLNRGQETDSD
                     ESVFSTRWLYGSLALLSAPQVESAGQVVPQLARVLIDGADLFAHVLVDLTGFELLGEH
                     ASAAACMDAVALVGLAHHTREKELLELARLMPHGRFFGVLLVGS"
     gene            4420..>4603
                     /locus_tag="VF516_43170"
     CDS             4420..>4603
                     /locus_tag="VF516_43170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8114631.1"
                     /translation="MLSIVHVLSSYGVGGQERVALDLAIGQKARGHEVAVISLAPPPD
                     GAMADEFAAAGVAVGRV"
BASE COUNT          676 a         1569 c         1617 g          741 t
ORIGIN      
        1 gggccggccc ggtcagcgtc gacggcgccg gcttgtcctc cggaagggcc ggcccggtca
       61 gcgtggtcgc gggccgcggc gcgtcggggt cgctgggctt cgccggcggc cggggctcgg
      121 gctcgtcacc gggcgcggcg acgatctcgg acgccgcctc gtggctgcgc atcagctcgt
      181 cctgggcatc ctccagggag ctcagccggt ggcgcagctc accgagccgc tcggacaggt
      241 cggcgagctg cgcgtgcagc tcgcgcgcgg tcttgtcgtc ggtctgggtc aggtcgggct
      301 gcagcacggc gagcgcgcgc tccagcaggt cgcggcggtc gctggcatag acgaactgat
      361 aggtatcggt actcttgccc tcgaccgcgg cctgcagcgc ctgcagggcc ctggtctcgc
      421 gcgccggcat gcggagctgc tcgatgtcgg ggcgctcgcg cccgaggatc atgcggatcg
      481 ccccgagcag ctgggtgcgc tgctcccggc ccgcctcgtg gatcgcctcg accacgggcg
      541 tcggcaagct gtccttgccg tccttgcgct ggcgctcctc gcgctgctgg agctccgcgt
      601 gagccgcctc gcggctttcg atgatcttgt ccttgaggtc caccgctcgt tcaccactag
      661 tcgccgcggt cggcgctagt cctccgtctt ctcccaccac tgacaccacc agctgccgcc
      721 gaccaggatt cgcagcttgg ggtgccagca gtacgagaag ttgtcgtcgc gatggagata
      781 atacatgcag ttatcgcagc gctcatcacc atacggctgc cccttcagca ccgcgtcctc
      841 ggcgaggtgc ctcagctgca acgcgagctt ctcgtcgatc tccttgggct ccggcttggg
      901 caatccatcg tctgccattt tcgcggcgcg agcttaccaa acttggctcg cgctggacgc
      961 gctgcgttgc gcgatcctta cctcgaaaaa tcaaggccga ggttcgctgc gcgatgcgat
     1021 gcgatgcgag gctcgttacc cgcgccgcag ccgatgagcg aaccaggcca cggtctctgc
     1081 cattccgtcc tcgaccatgt gggatggcac gtacccgagc gccgcgctga tcttgtcgat
     1141 cgccgcctgc gaatgggcga cgtcccccgc gcggggcgcc tcgaacaccg gctcggcgcc
     1201 ggcgacctcg ggcatggtct tggcgaggtt gtcgcggatc atcacgaaca gctgcttgag
     1261 atcggtgcgg ccgttgcagc cgcagttgta cacggtgccg gtgacggcag gatccggcac
     1321 ggtcgccgcc aggagattgg cctggacgat attgtcgaca tagcaaaagt cccggctgtt
     1381 cgcgccgtcg ccgaagatca cacacggctt gccggccacc agatttgcga cccagcgcgg
     1441 gatcaccgcg gcgtagggac cgtcggggtc ctggcgacgg ccgaacacgt tgaagtagcg
     1501 cagcccgatc agctgctggc cgtagacgtc gtggaacacc tgggcgtaga tctcgtcggc
     1561 gcgcttggtc gcggcgtacg gcgacagcgg cttgccgatc cggtcctcgt gcttgggcag
     1621 cccggggtga tcgccgtaga ccgacgacga cgacgcgtag accaggcggc cgacgccggc
     1681 gtccttggcc gcccacagca cgttgaggaa cgcatcgacg ttgtgggcgt gcgaggccag
     1741 cggatccttg atcgaccgtg gcaccgagcc gagcgcggcc tcgtgcagca cgatgtcgac
     1801 gtccttgcac gcctcgcggg cgaccgccgg atcgcgcaga tcgccctcga tgaagcggaa
     1861 ctgcagccgc tcgtcggggt tgatcgcgag cacgtcgtcg aggttgcgct tgtggccggt
     1921 caggaagttg tcgaccccga ccacgctctg gccgagatcg agcaggcgct cgagcagcgc
     1981 cgagccgatg aaccccgcga ccccggtcac cagccagcgg cgcggcgagt tgaccagctc
     2041 ctcgcagacg tcttcgtagc gtgtgagctt catagactcc agagctggat tccccgcggt
     2101 ggcttgacct gcgccggcag gacactcttg acgtcgatca cgaccccgtt gtcgcgcacc
     2161 cgctcgtaga tcgcgccggc gttgccgagg tacgccttgt gggcgaccgc gaggatcacg
     2221 gcgtcgaggt tcaccagctg ctcgagcggg gtcagccgga tcttgtactc gtggtaggcc
     2281 tcctcggggt tgcccagcgg atcgtggacc atcgcctgga tcccgaacgt cgccagctcg
     2341 gtgacgatgt cggggatctt ggagttgcgg aggtcgggca cgttctcctt gaaggtcagg
     2401 ccgaggatgc cgacccgcgc cttcctgagc gcgacgtcgg agtgggtcag catcttgacg
     2461 cacttctggg ccaggaacgg gccgacgttg ttgttgatcc gccggccggc gaggatgacc
     2521 tcgggcagat agccgagctc ctcggccttg gtcgtcaggt agtacgggtc gacgccgatg
     2581 cagtggccgc cgaccagacc cggggcgaac ttgaggaagt tccacttggt gccggcggcc
     2641 tcgagcacgt cggcggtccg gatccccatg cggtcgaaga tcagcgcgag ctcgttcatc
     2701 agcgcgatgt tgagatcgcg ctgggtgttc tcgatcacct tggcggcctc ggcgaccttg
     2761 atcgacggcg ccttgtggac accggcggtg accaccgagc cgtagacccg cgcgacgcga
     2821 tcgaggatct cgggggtctg gcccgacacg accttgagga tcttgtcgaa cgtgtgctcc
     2881 ttgtcgccgg ggttgatccg ctcgggcgag tagcccagga agtagtcggt gccgttcttg
     2941 agcccgctgc cctgatcgag cagcggcccg cacacctcct cggtgacgcc ggggtacacc
     3001 gtggactcgt agaccacgac gtcgccgggg cgcaggtgcg gcgcgatcgt cttgctcgcc
     3061 gagatcaccg gcccgaggtc ggggcggttg ttgccgtcga tcggggtcgg gaccgccacg
     3121 atgtagaacg tgcagtcggc gagccgggtc ggatccgcgg tcatctccag cagcctggcc
     3181 gacgcgagct ggtcgtcggc gacctccagc gtgtcgtcgt ggccctgctt gagctggtcg
     3241 acgcggcgct gccggatgtc gaagccgacc gtcgacagct tcttgccgaa cgcgatcgcc
     3301 accggcaagc cgacgtagcc cagaccgatc accgcgatct tctcgtgttc caaaggccgc
     3361 attgctagaa agatgagcac gatcgacgac agcagttcaa gcgatcgcgc cgccagcctg
     3421 cgatcgcgcg cgcgaccgcg tgagtgcgct cccacgcacg ctgggcgcga cgcgcacagt
     3481 ggcgacgacc gtggcgacga tcgtgggcga cgatcgcggc cgcgaccgcg gctacgatcc
     3541 cacgagcagg acgccgaaga accggccgtg cggcatcagc cgcgccagct ccagcagctc
     3601 cttctcgcgg gtgtggtggg ccaggccgac cagcgcgacc gcgtccatgc acgcggcggc
     3661 cgacgcgtgc tcgccgagca gctcgaaccc ggtgagatcg accagcacgt gggcgaacag
     3721 atcggcgccg tcgatcagca cccgggcgag ctgggggacg acctggccgg cgctctcgac
     3781 ctgcggcgcg gacaagagcg cgagcgagcc gtacagccag cgggtcgaga acaccgactc
     3841 gtcggagtcg gtctcctggc cgcggttgag catgccgagc cccgggtagc gcacgttggc
     3901 gtcgaccacg gcgatcgtgg cgccggtcag ctcggtgagc gcgatgccga gctggatcag
     3961 cgccggcggc acggcgacgg tgtcctcggc cgggacgaac ccgacgaccc ggacgccctc
     4021 gttgtggatc cggcgcgcca cccgcgtgca cgccgcccgg agctcgccgc cgcccggttc
     4081 gatcccgagc gaggccagca ccgcgtcgtc gggtttgctg cccggccgga cgaccgccgc
     4141 ggtacgcggc ggtcgtctgc gcaggcgcca ccatatcagt gcgtcacgcg ccgctgcgac
     4201 ggcggccacc gccagcgcga tctgcgacat ggcggggatc ggtgcccgga accagatcgc
     4261 agcgatcacc gcgaccgaca ggccgactag gaagcgaaca cgtacgcgta cgatcgacac
     4321 gaccacggac agcatagcct gtaccggacc ggcgcgccgg acggtcgcct cacgatgtac
     4381 cggcttgccg gaccggtgcc gcacgggtac gcaaaatgag tgctgtcgat cgtccacgtc
     4441 ctgtcctcgt acggggtcgg cgggcaggag cgcgtggcgc tcgatctggc gatcggtcag
     4501 aaggcgcgcg gccacgaggt cgcggtgatc tcgctggcgc cgccgcccga cggcgccatg
     4561 gccgacgagt tcgccgccgc cggcgtcgcg gtcggccggg tcg
//
LOCUS       DASVOR010002343         2565 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_287459,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002343 DASVOR010000000
VERSION     DASVOR010002343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287459"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="VF516_43175"
     CDS             <1..552
                     /locus_tag="VF516_43175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114632.1"
                     /translation="AAIEVDHRLGDRLGARPGRLARGVAALTALPAIGFFAFNLVFEP
                     ITESFGAPDPDFRAPAAWVRAHTGRDDRVFVWGMFAPIYVLADRLPASRFVGFLRGAE
                     RNRGVAPDTSWDTGPEVWPALAEDFARHRPAVVIDTATADYLSFGRYPIRRFPAVAAL
                     LRDYRVAAVVDGVTMYVPAAVSP"
     gene            complement(542..2047)
                     /locus_tag="VF516_43180"
     CDS             complement(542..2047)
                     /locus_tag="VF516_43180"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8114633.1"
                     /translation="MRAWLLAIAVVGCGGTSDESQPVPSPPPRPVELEPAPPAPPDFP
                     EGTRSLELRRTVGVRLEPGDDAKRIGNVAVDTRVAWVRTQAGRGCDRPWIEIRPRGWI
                     CGDYVEPSKKPPYGQEVPHLDRGELVPGIYGKVTAPASVTYALEKPGQKKPARKGANA
                     RKDSREARKDPRSSEDRRRQARGVEDTAPLAPPHMVEDKPLVGSVNVRQYEEVAVGGR
                     LYWRIDKKDNEYVLAAAIALHHPSSFGGLRLGDDTGMTAALGFVSPRFGAGQAYTTNT
                     AVGGGINRQLAARTPLAILETAADKAGKPTAYRIGAAEWIAAADTRVFQPAPPPPLLL
                     PGERWIDVDIDDQILVAYEGDLAVYATLVSSGGTTTPTETGVYRVWLKESEADMKGLN
                     GEDPYSVATVPWTEFFSPEKGLALHTAYWHDQFGTRRSHGCVNLAPRDARWLYFWSDP
                     QVPAGWTMAAGMPEAPGSIVRIRTRDDPAPEAKGYAKKVLEARQQNAPVKG"
     gene            2087..>2565
                     /locus_tag="VF516_43185"
     CDS             2087..>2565
                     /locus_tag="VF516_43185"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114634.1"
                     /translation="MRLGEMLVRDGRLTEADLADALGYQARDGGRLGTVLFEHGMVDL
                     EALTVYLGLELGIPIATGAMLERAKRAAVRLLQPPQAFKHRCVPLVVQDRQLIAAIED
                     PHDFAILDALASITGYRVLPRVAPELRIYYYIERYYGVARPARFQHFGDSPRGNEAT"
BASE COUNT          300 a          980 c          900 g          385 t
ORIGIN      
        1 gccgcgatcg aggtcgatca ccgcctcggc gaccggctgg gcgcgcggcc cgggcggctc
       61 gcccgcggcg tggcggcgct gaccgcgctg cccgcgatcg ggttcttcgc gttcaacctc
      121 gtgttcgagc cgatcaccga gtcgttcggc gcgccggatc ccgacttccg cgcgccggcg
      181 gcgtgggtcc gcgcgcacac cgggcgagac gaccgggtct tcgtctgggg catgttcgcg
      241 ccgatctacg tgctcgccga ccggctgcct gcgtcgcgct tcgtcggctt cctgcgcggc
      301 gccgagcgca accgcggggt cgcgccggac accagctggg acaccggccc ggaggtatgg
      361 ccggcgctcg ccgaggactt cgcgcgccac cgccccgccg tggtgatcga caccgcgacc
      421 gccgactacc tgagcttcgg gcgctatccg atccggcggt tcccggcggt ggcggcgctg
      481 ctgcgcgact accgcgtcgc cgcggtggtc gacggcgtga cgatgtacgt gccggccgcg
      541 gtcagccctt gaccggcgcg ttctgctggc gcgcctccag caccttcttc gcgtagccct
      601 tggcctcggg agccggatcg tccctggtgc gaatccggac gatcgagccc ggcgcctcgg
      661 gcatgcccgc cgccatcgtc cagcccgccg ggacctgcgg atcgctccag aagtacagcc
      721 agcgcgcgtc gcgcggcgcg aggttgacgc agccgtggct gcgccgcgtg ccgaactggt
      781 cgtgccagta cgcggtgtgc aacgcgaggc ccttctccgg cgagaagaac tcggtccacg
      841 gcacggtggc gaccgagtac gggtcctcgc cgttgagccc cttcatgtcg gcctcggact
      901 ccttgagcca cacgcggtac acgccggtct cggtcggcgt ggtggtgccg cccgacgaca
      961 ccagcgtcgc gtagaccgcg aggtcgccct cgtaggcgac caggatctgg tcgtcgatgt
     1021 cgacgtcgat ccagcgctcg ccgggcagca gcagcggcgg cggtggcgcg ggctggaaca
     1081 cccgggtgtc ggccgcggcg atccactccg cggcgccgat ccggtacgcg gtcggcttgc
     1141 cggccttgtc ggccgcggtc tcgaggatcg cgagcggggt gcgcgcggcg agctggcggt
     1201 tgatcccgcc gcccaccgcg gtgttcgtgg tgtacgcctg cccggcgccg aaccgcggcg
     1261 acacgaaccc cagcgccgcc gtcatcccgg tgtcgtcgcc gagccggagc ccgccgaacg
     1321 acgacgggtg gtgcagcgcg atcgcggcgg cgagcacgta ctcgttgtcc ttcttgtcga
     1381 tcctccagta cagccggccg ccgaccgcaa cctcctcgta ctgccggacg ttgaccgagc
     1441 cgaccagcgg cttgtcctcg accatgtgcg gcggcgcgag cggcgccgtg tcctcgacac
     1501 cgcgcgcctg ccggcgccgg tcctccgacg atctcggatc cttgcgcgcc tcgcggctgt
     1561 ccttgcgggc gttcgcgccc ttgcgcgccg gcttcttctg ccccggcttc tcgagcgcgt
     1621 acgtcaccga ggccggcgcg gtgaccttgc cgtagatccc cggcaccagc tcgccgcggt
     1681 cgaggtgcgg cacctcctgg ccgtacggcg gcttcttgct cggctcgacg tagtcgccgc
     1741 agatccagcc gcgcggccgg atctcgatcc acggccggtc gcagccccgg ccggcctggg
     1801 tccgcaccca ggcgacccgc gtgtcgaccg cgacgttgcc gatccgcttg gcgtcgtcgc
     1861 ccggctcgag ccgcacgccg accgtccggc gcagctccag cgatcgcgtg ccctccggga
     1921 agtcgggcgg cgcgggcggc gccggctcga gctcgaccgg gcggggcggt ggcgatggca
     1981 ccggctgcga ctcgtccgac gtgccgccgc aaccgacgac ggcgatcgca agcagccagg
     2041 cgcgcatccc gttgcgatag cacgtccatg ataccttcgc aggcagatga ggctcggcga
     2101 gatgctggtg cgcgacggcc ggttgaccga ggccgatctc gcggacgccc tcggctacca
     2161 ggcgcgcgat ggcggccggc tcggcaccgt gctgttcgag cacggcatgg tcgatctgga
     2221 ggccctcacg gtgtacctcg gcctggagct cggcatcccg atcgccaccg gcgcgatgct
     2281 cgagcgcgcc aagcgcgccg cggtgcggct gctccagccc ccgcaggcgt tcaagcacag
     2341 gtgcgtcccc ctggtcgtcc aggaccgcca gctgatcgcc gcgatcgagg acccccacga
     2401 cttcgcgatc ctcgacgccc tggcctcgat caccggctac cgcgtgctgc cgcgcgtcgc
     2461 ccccgagctc cgcatctact actacatcga gcgctactac ggcgtcgccc gccccgcgcg
     2521 cttccagcac ttcggcgact cgccgcgcgg taacgaggcc acaat
//
LOCUS       DASVOR010002344         1909 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_201116,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002344 DASVOR010000000
VERSION     DASVOR010002344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1909
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201116"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1909)
                     /locus_tag="VF516_43190"
     CDS             complement(<1..>1909)
                     /locus_tag="VF516_43190"
                     /inference="COORDINATES: protein motif:HMM:NF019136.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HEX8114635.1"
                     /translation="RIESVLRDRDGMLWIRSLHHLWTLPRGAAQVVDLSDGLPAGSDM
                     SGVSCGMAISPSGDVLVGSERGVARRRAGRWQLLDASVGVPVRQARTLFVDREGTVWI
                     GSMGLFQWMGRGLIAHHNMATGLPDDTVWTISRDRHDVLWLGTGKCLARAIDGRWSCL
                     PASVGWSVRTFVFLPQGGVFIGGGPPDLLYIDPAGQATTLELQGEQVADRQIMAAALG
                     PEGDLWLATTSGLYRLPGAQPGHPARVAIPGAKVDTRVISVLVVDGRLWAASDSGLAV
                     LDHGTWRLLDRSAGFRASAMRHVIRRRDGRMCVSFTDVDGLTCFQWDGSRASDLVDIS
                     SADGLTSGRIYFLGEDRQRRLWIGTGDGVDVVTASGVDHFNETDGLAGNDSTARAFFD
                     DSDGSIWLGASTGVSHVLAQHYRGPPIAPRTTVLRSALGGSPVPAGLTAPIQVPHERN
                     SVTASFAADSFIDPRRVEFQVRLSPLESEWSTTRLREARYLALPPGSYQLEMRARTGA
                     GAWGRPAELRFAVLPAWWQTRWFIVIAGGLALAGLGGVVTWRQRVLWRRRARQLTQQS
                     DARFRELIEAMPDLVSVHRDDQLIYLNQAARQMLGAGATRAPRQVNLVDRIHPDDRPR
                     AAKLFRDARTDA"
BASE COUNT          264 a          694 c          722 g          229 t
ORIGIN      
        1 gcgtcggtcc gggcgtcgcg gaacagcttg gcggcgcgcg ggcggtcgtc ggggtggatc
       61 cggtcgacca ggttgacctg gcgcggtgcg cgggtcgcgc cggcgcccag catctggcgc
      121 gccgcctggt tgaggtagat cagctggtca tcgcggtgga cggacaccag gtcgggcatc
      181 gcctcgatca gctcgcggaa ccgggcgtcg gactgctggg tgagctgccg ggcgcggcgc
      241 cgccacagca cgcgctgccg ccaggtcacc acgccgccca ggccggccag cgcgaggccg
      301 ccggcgatca cgatgaacca gcgggtctgc caccacgcgg gcagcacggc gaaccggagc
      361 tcggcgggcc ggccccacgc gcccgcgccg gtgcgcgcgc gcatctcgag ctggtagctg
      421 ccgggcggca gcgccaggta acgcgcctcg cgcagccggg tcgtgctcca ctcgctctcg
      481 agcggcgaca gccggacctg gaactcgacc cgcctgggat cgatgaagct gtcggcggcg
      541 aagctcgcgg tgaccgagtt gcgctcgtgc gggacctgga tcggcgcggt caggccggcc
      601 gggaccggcg agccgccgag cgcgctgcgc aggacggtgg tgcgcggcgc gatcggcggt
      661 ccgcggtagt gctgggccag gacgtgggac acgccggtgc tggcgccgag ccagatcgag
      721 ccgtcgctgt cgtcgaagaa cgcgcgcgcg gtcgagtcgt tgccggccag gccgtcggtc
      781 tcgttgaagt gatcgacgcc gctcgcggtg accacgtcga cgccgtcgcc ggtgccgatc
      841 cacagccggc gctggcgatc ctcgccgagg aagtagatcc gcccggaggt caggccgtcg
      901 gccgagctga tgtcgaccag gtcggaggcg cggctgccgt cccactggaa gcaggtcagg
      961 ccatcgacgt cggtgaacga cacgcacatc cggccgtcgc gccgccggat cacgtggcgc
     1021 atcgccgagg cgcggaaccc cgcgctgcga tcgagcagcc gccaggtccc gtggtccagc
     1081 accgccagcc cggagtcgct cgcggcccac agccggccat cgacgaccag caccgagatg
     1141 acgcgggtat ccaccttggc ccccgggatc gcgactcgcg cggggtgccc gggctgcgcg
     1201 ccgggcaggc ggtacaggcc cgacgtggtc gccagccaca gatcgccctc gggcccgagc
     1261 gcagcggcca tgatctggcg atcggcgacc tgctcgccct ggagctccag ggtcgtcgcc
     1321 tggccggcgg gatcgatgta cagcaggtcg ggcgggccgc cgccgatgaa cacgccgccc
     1381 tgcgggagga acacgaacgt ccgcacgctc cagccgacgc tggcgggcag gcagctccag
     1441 cggccgtcga tggcgcgcgc gaggcacttg ccggtgccga gccacagcac gtcgtggcga
     1501 tcgcggctga tcgtccacac cgtgtcgtcg ggcagcccgg tggccatgtt gtggtgggcg
     1561 atcaggccgc ggcccatcca ctggaacagc cccatcgagc cgatccagac cgtgccctcg
     1621 cgatcgacga acagcgtgcg ggcctggcgc accggaacgc cgaccgaggc gtcgagcagc
     1681 tgccagcggc cggcgcggcg ccgggccacg ccgcgctcgc tgccgaccag gacgtcgccc
     1741 gatgggctga tcgccatgcc gcacgacacg ccgctcatgt ccgagccggc gggcagcccg
     1801 tcgctcagat cgacgacctg cgcggcgccg cgcggcagcg tccacaggtg gtgcagcgag
     1861 cggatccaca gcatgccgtc gcggtcgcgc agcacgctct cgatgcgct
//
LOCUS       DASVOR010002345         1685 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_404702,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002345 DASVOR010000000
VERSION     DASVOR010002345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1685
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404702"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VF516_43195"
     CDS             <1..532
                     /locus_tag="VF516_43195"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEX8114636.1"
                     /translation="DAHLRLLDASSDEAYVENAGVVLLWPFLRTLFERLELTADRRFE
                     DAAAQGRAAGLLQHLVTGELEPLEHELALAKVLCGLAPAELIELDPPVTDVEADECTQ
                     LLTAAIANAPILGDLSIAGFRGSFLIRNGVLGTRDGVWWLRVERAPYDVVLDQLPWSV
                     SWVRLPWMETPLAVEW"
     gene            543..>1685
                     /locus_tag="VF516_43200"
     CDS             543..>1685
                     /locus_tag="VF516_43200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114637.1"
                     /translation="MRIAIEKQAAPETARSTRSAERAPSAKAAGPARLDDAPAEARLE
                     RAFGPDQLAAPAQRAGAPQVQRAPQSTAQGDVATLPLEEALRRLPTLPPAAALGVLKR
                     FQSTIVIRISTGERRIQLAMSQRAESFGNYLIGGAIEVFGLTSLPSAQWEEAWKPLND
                     AYATIARREVKASLDLLLAAAAATNRYWEQLNAYLEKTDKGADRSIFALQVLEAAGAV
                     AAAALTGGGAAAIAVGAGYAATQNLVGQATSVSIGIQDKIDWAGLAFDTVFGAITGAL
                     GGKLGNVVLKKLMGNPAVASVSRRLLAEVVSDLISGRLSSLLQTTAHTLFNQLRGRET
                     LTMEQFIDRLGDQLMDPKAMFLDAILGRAAKLTQARRAPPGAPAAHE"
BASE COUNT          259 a          600 c          585 g          241 t
ORIGIN      
        1 cgacgcgcac ctccgcctgc tcgacgccag ctcggacgaa gcgtacgtcg agaacgcggg
       61 tgtggtcctg ctctggccgt tcctccgtac cctgttcgag cgactcgagc tgacggccga
      121 tcgccggttc gaggatgccg ccgcccaggg ccgtgcggcc ggcctgctgc agcacctggt
      181 caccggcgag ctcgagccgc tcgaacacga gcttgccctg gccaaggtgc tgtgcggcct
      241 cgcaccggcg gagctgatcg agctcgaccc accggtgacc gacgtcgagg ccgacgagtg
      301 cacccagctg ctcaccgccg cgatcgcgaa cgcgccgatc ctcggcgacc tgtcgatcgc
      361 ggggttccgc ggatcgttcc tgatccgcaa cggcgtgctg gggacgcggg acggcgtctg
      421 gtggttgcgc gtcgagcgcg cgccgtacga cgtcgtgctg gatcagctgc cgtggagcgt
      481 gagctgggtg aggctaccgt ggatggagac gccactcgcc gtggagtggt gaaggagtca
      541 tcatgcggat cgcgatcgag aagcaagcgg ccccggaaac cgcccgctcc acgcggagcg
      601 ccgaacgcgc gccttccgcg aaggccgccg gccccgcgag gctcgacgat gcgccagcgg
      661 aggcgaggct cgaacgagcg ttcggcccgg atcagctggc cgctccagca cagagggccg
      721 gcgcgcccca ggtgcagcgc gccccgcagt ccacggcgca gggcgacgtc gccaccctgc
      781 cgctcgaaga agcgctgcgc cggctcccca cgctgcctcc ggctgccgcc ctcggcgtgc
      841 tcaagcgatt tcagagcacc atcgtgatcc ggatctcgac gggcgagcgc cggatccagc
      901 tagcaatgtc acagcgcgcc gagagcttcg gtaactacct gatcggaggc gcgatcgagg
      961 tgttcggcct cacctcgctg ccgtccgcac agtgggagga ggcgtggaag ccgctgaacg
     1021 atgcctatgc cacgatcgcc cggcgcgagg tgaaggcgag cctggatctc ctgctcgctg
     1081 ccgcggcggc caccaaccgc tactgggaac agctcaacgc ctacctcgag aagaccgaca
     1141 aaggcgctga ccgctcgatc ttcgccttgc aggtgctgga agcggcgggc gcggtcgccg
     1201 ctgcggcgtt gaccggcggc ggcgccgccg cgatcgccgt cggcgcgggc tacgccgcga
     1261 cccagaacct ggttggccag gccacctcgg tgagcatcgg catccaggac aagatcgact
     1321 gggccgggct cgccttcgac accgtgttcg gcgccatcac cggtgcactc ggcgggaagc
     1381 tcggcaatgt cgtgctgaag aagctcatgg gcaacccggc ggtggcctcg gtgagccgcc
     1441 gcctcctggc tgaggtcgtc tccgacctga tctccggccg gctgtcgtcg ctcctgcaga
     1501 cgactgcgca cacgctgttc aaccagctgc gcggtcgcga gacgctgacc atggagcagt
     1561 tcatcgaccg cctgggcgat cagctgatgg atcccaaggc catgttcctc gacgcgatcc
     1621 tcggccgcgc cgcgaagctg acacaggctc gcagggcccc gcccggcgcg ccggcagcgc
     1681 acgag
//
LOCUS       DASVOR010002346         1716 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_346529,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002346 DASVOR010000000
VERSION     DASVOR010002346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346529"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1077)
                     /locus_tag="VF516_43205"
     CDS             complement(<1..1077)
                     /locus_tag="VF516_43205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEX8114638.1"
                     /translation="MMAIPNLGAMLPALLEVLSYVGTGVTVLADRGQGIERVYINEAS
                     AAAMGYTLDELRTQPVEESIAPEQRALLAEMLAGFRAGKPVPPVIEFTAVRKDGTTIT
                     LELAISSFRIPDGVAYVMLLRDGPHTQSRLSLLEADRIGLVGALSAGFAHEINNPLTS
                     VLLNLRLLRRHLTTGMSLTSQPAALRCLDDIATGAERIASNVSALQSLATRRATAPID
                     LAAVVSAALRLAAPTLEERAHVIRQIFPVRKVNGEESRIGQAVIAMLLFSSSGFDGET
                     TNISNRIVVSVEQRDDDVVLEVSDNGRELTPEEADHAFDPFYRSSTRGASIGVGLGVA
                     RSVAATLGGEVTLAPRPGGGAVITM"
     gene            complement(1074..1592)
                     /locus_tag="VF516_43210"
     CDS             complement(1074..1592)
                     /locus_tag="VF516_43210"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012913.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase domain-containing protein"
                     /protein_id="HEX8114639.1"
                     /translation="MTRRIALISTGGTIEKTYDELRGVLENQVSVLDVMLASLELRGV
                     AVHRVPLMNKDSLDMSPEDHTLIAHTATRLAETHAGVVVVHGTDRLAVSGHRVVEVAG
                     TPAAPIVFTGAMRPYELRSTDALQNLTEALLAVQLVPPGVYVAMHNQVLRFPGVVKDP
                     ERRTFVKVAEAG"
BASE COUNT          246 a          575 c          649 g          246 t
ORIGIN      
        1 catcgtgatc accgcgccac cgccgggccg cggcgccagc gtgacctcac cgccgagcgt
       61 ggcggcgacc gagcgggcga cgccgagccc gacgccgatg ctcgcgccgc gggtcgacga
      121 ccggtagaac gggtcgaacg cgtggtcggc ctcctcggga gtcagctcgc ggccgttgtc
      181 ggacacctcg agcacgacgt cgtcgtcgcg ctgctcgacg ctgaccacga tccggttgct
      241 gatgttggtg gtctcgccgt cgaaccccga gctcgagaac aagagcatcg cgatcacggc
      301 ctggccgatc cgcgactcct cgccgttgac cttgcggacc gggaagatct ggcggatcac
      361 gtgggcgcgc tcctcgaggg tcggcgccgc gagccggagc gccgccgaca ccacggcggc
      421 gaggtcgatc ggcgcggtcg cgcgccgggt cgcgaggctc tgcagcgcgc tgacgttgct
      481 cgcgatgcgc tcggcgccgg tggcgatgtc gtcgaggcag cgcagcgcgg cgggctggga
      541 cgtgagcgac atcccggtgg tcaggtggcg gcgcagcagg cgcaggttga gcagcacgga
      601 ggtcagcggg ttgttgatct cgtgggcgaa cccggccgac agcgcgccga ccaggccgat
      661 ccggtcggcc tcgagcagcg atagccggct ctgggtgtgg ggaccgtcgc gcagcagcat
      721 gacgtaggcg acgccgtcgg ggatccggaa cgagctgatc gcgagctcca gggtgatcgt
      781 cgtgccgtcc ttgcggacgg cggtgaactc gatcacgggc ggcaccggct tgccggcgcg
      841 gaagccggca agcatctcgg ccagcaacgc gcgctgctcg ggcgcgatcg actcctccac
      901 cggctgggtg cggagctcgt ccagcgtgta gcccatcgcc gcggcggacg cctcgttgat
      961 gtacacgcgc tcgatcccct ggccgcgatc ggcgagcacg gtgacgccgg tgccgacgta
     1021 gctgagcacc tcgagcagcg ccggcagcat cgcgccgagg ttggggatcg ccatcatccg
     1081 gcctcggcga ccttcacgaa ggtccggcgc tcggggtcct tgaccacgcc ggggaaccgg
     1141 aggacctggt tgtgcatggc gacgtagact ccgggcggga cgagctggac cgcgagcagc
     1201 gcctcggtca ggttctgcag cgcgtcggtc gaccgcagct cgtacggccg catcgcgccg
     1261 gtgaacacga tcggcgcggc cggcgtgccg gcgacctcga cgacgcggtg gccggacacc
     1321 gcgagccggt cggtgccgtg gacgacgacc acgccggcgt gggtctcggc cagccgcgtg
     1381 gcggtatggg cgatcagcgt gtggtcctcg ggcgacatgt cgaggctgtc cttgttcatc
     1441 agcggcacgc ggtggaccgc gacgccgcgc agctcgagcg atgcgagcat gacgtcgagc
     1501 accgagacct ggttctccag gactccgcgg agctcgtcgt aggtcttctc gatcgtaccg
     1561 ccggtcgaga tcagggcaat gcgtcgggtc atgttggtca actcgctggc ttccccatca
     1621 cgcgcgcgct ccgtcaggtc agcaggagga ggacgaagta ctccgtggtc gcggcggagg
     1681 cggtggcgag caccacgagg atcaccgccg cgaggg
//
LOCUS       DASVOR010002347         2039 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_142547,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002347 DASVOR010000000
VERSION     DASVOR010002347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2039
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142547"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..781)
                     /locus_tag="VF516_43215"
     CDS             complement(179..781)
                     /locus_tag="VF516_43215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEX8114640.1"
                     /translation="MRTDAADEDLMVLYQKGEVRAFELLLTRHRKPVYNFILRFVGDK
                     ETAEDLLQEAFMRVIKGAEAYKRQAKFTTWLYTIARNLCVDQTRRRKHRKHASLDAPM
                     DTSDDSGTLLDVIPSNEMASDRRSLNKQLHETMHRAISALSEEQREVFLMREFLDLPF
                     KQIADIVGVPENTVKSRMRYALEKLRLDLDEYKDVAKAAP"
     gene            complement(866..1690)
                     /gene="mutM"
                     /locus_tag="VF516_43220"
     CDS             complement(866..1690)
                     /gene="mutM"
                     /locus_tag="VF516_43220"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEX8114641.1"
                     /translation="MPELPEVETVRRTLAPAVGGQILSVWTSVMGLHMARRPPVARLR
                     KLVGGRITAVRRHGKYLLVDTDRGAAILVHLGMTGRLRLHAASDPRAPHTHVVIGLGR
                     RELRFVDPRRFGQVDVVARGKEREHPGLAVLGPDALDQAIDVAAMLARARDKRTTLKA
                     FVLDQAVIAGVGNIYASEALWRARLRPTMRTHKLTAASATRLAAAIREVLERALDNGG
                     TTLSDFVDADGTAGENADYLWVYDRAGQPCERCRTAIKRSVLQGRATYYCPTCQTP"
     gene            1768..>2039
                     /locus_tag="VF516_43225"
     CDS             1768..>2039
                     /locus_tag="VF516_43225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114642.1"
                     /translation="MPKRPPRVARELPVPDDLSAPGPTCALDLAAGGAPDAAERDERG
                     PGRGELWHADVLELLGKLADGSVDLVIADPPYAIAKAAWDEFESLEA"
BASE COUNT          282 a          703 c          715 g          339 t
ORIGIN      
        1 ggcgggtcct cgtcgcgcat cgccagccag accgcgcgca tctggcgcag ctgagggtcg
       61 ctgccggcct cgacggcgcg cgcgttctcc ggtgcgtcgt ccggcgcgcc gtcgcgacct
      121 tgccggggca tggcggtacc gcgcggggcg tcgtcgtcgc gcgacccacc ggggggcgtc
      181 acggtgcggc cttcgcgacg tccttgtact cgtcgagatc gagccgcagc ttctccagcg
      241 cgtagcgcat ccggctcttg accgtgttct cgggcacacc gacgatgtcg gcgatctgct
      301 tgaacggcag gtcgaggaac tcgcgcatga ggaagacctc gcgctgctcc tccgacagcg
      361 cggagatcgc ccggtgcatg gtctcgtgca gctgcttgtt cagcgaccgg cgatcactgg
      421 ccatctcgtt cgacggaatc acgtccaaca aggtcccgct gtcgtcgctg gtgtccatcg
      481 gtgcgtcgag cgaggcatgc ttgcggtgct tgcgtcgcct ggtctggtca acgcacaggt
      541 tgcgggcaat ggtgtacagc cacgtcgtga acttggcctg ccgcttgtaa gcctcggcgc
      601 ccttgatgac gcgcatgaag gcttcctgca gcaggtcctc ggcggtctcc ttgtcgccga
      661 cgaaccgcag gatgaagttg tagaccggct tgcggtggcg ggtcaaaagc agctcgaacg
      721 cacggacctc gcccttctgg tacaggacca tgaggtcttc atcggcggcg tcggtcctca
      781 tgcggagtgg ttcggggggt atcttacctg taagccgcgg ggcaaacgca cgctgcgatc
      841 cacaacgcgc caagccggag accgcttacg gggtctgaca cgttggacag taatatgttg
      901 cccggccttg gaggacagat cgtttgatcg cggtccggca gcgctcgcac ggctgtccgg
      961 cccgatcgta gacccacagg tagtcggcat tctcgcccgc cgtgccatcg gcatcgacga
     1021 agtcggagag cgtcgtgccg ccgttgtcga gcgcccgctc gagcacctcg cggatcgccg
     1081 ccgccaggcg cgtcgccgag gcggcggtca gcttgtgggt ccgcatcgtc gggcgcagcc
     1141 gtgcgcgcca cagcgcctcc gacgcgtaga tgttgccgac cccggcgatc acggcctggt
     1201 cgagcacgaa cgccttgagc gtggtgcgct tgtcgcgggc gcgggccagc atcgcggcga
     1261 cgtcgatcgc ctggtcgagc gcgtccggcc cgagcacggc gagcccggga tgctcgcgct
     1321 ccttgccgcg cgccacgacg tcgacctggc cgaaccggcg cgggtcgacg aaccgcagct
     1381 cgcgccggcc gagcccgatc acgacgtggg tgtgcggcgc gcgcggatcg gacgccgcgt
     1441 gcagccgtag ccgtccggtc atgccgaggt ggaccaggat cgccgcgccg cgatcggtgt
     1501 cgaccagcag gtacttgccg tggcggcgga ccgcggtgat ccgtccgccg accagcttgc
     1561 gcagccgggc gaccggcggc cgccgagcca tgtgcaagcc catcacgctg gtccacaccg
     1621 ataagatctg cccgccgacc gcgggcgcga gcgttcttcg gacggtctcg acctccggta
     1681 gctccggcat ggctgccgca tccttagcat ttctggagca tttctggccg cggtggcgtc
     1741 agaccaaccg gttagactgt acgttcgatg cccaagcgcc ctccgcgcgt cgcccgcgag
     1801 ctgcccgtcc cggacgatct gagcgcgcca ggtccgacct gcgcgctcga cctcgccgcg
     1861 ggcggcgcgc ccgacgcggc cgaacgggat gagcgcggcc ccggccgggg tgagctgtgg
     1921 cacgccgatg tcctggagct gctcggcaag ctcgcggacg gctcggtcga tctggtgatc
     1981 gccgatcccc cgtacgcgat cgcgaaggcc gcgtgggacg agttcgaatc gctcgaggc
//
LOCUS       DASVOR010002348         2242 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_142552,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002348 DASVOR010000000
VERSION     DASVOR010002348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142552"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1608)
                     /gene="shc"
                     /locus_tag="VF516_43230"
     CDS             complement(<1..1608)
                     /gene="shc"
                     /locus_tag="VF516_43230"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HEX8114643.1"
                     /translation="MAEREIQRTRASAHVDVDQVIARAHGWFRRAQFEDGYWWAELES
                     NATMDAEYILMTHFLGARDAAIWRGVAQDIRSYQQGDGSWALYAGAPGDLSTTVECYF
                     ALRLTGDSGPHLARARQFIAARGGIARARVFTRIWLALCGEWSWDDLPAMPIELMLLP
                     PRAPLSIYRFASWARGTIVPLLVLMNDRPVRRVPASASLADLRIGAPVAIEPRDAADR
                     LFLGLDAVLRRYHRAPWHPLRSRARRAAEHWLLDHQEADGSWGGIQPPWVYSMMALHA
                     LGYAADHPVIRRGLDGMHGRWMIRRSDGSLRIQACLSPVWDSALALVALRESGVPAHD
                     PAVQRAARWLVREEVRVPGDWQIAVPGVAPSGWSFEFDNDLYPDVDDTALVLIGLHRA
                     GALDEPTRARAIAWLLAMQCKTGGWAAFDKDNTSRLLARIPFADFGEMIDPPTADVTA
                     HVVEMLGEFGVPRSHPAVAAAVDYLYAEQEPDGSWFGRWGVNHLYGTGAVLPALAAIG
                     EPMDSLPVRRAVRWLVERQNPDGGFGEG"
     gene            complement(1595..>2242)
                     /locus_tag="VF516_43235"
     CDS             complement(1595..>2242)
                     /locus_tag="VF516_43235"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8114644.1"
                     /translation="GMRGGVLPDERQRATGAPGPYKQSKWRAEQLVADAAARGLDAVI
                     VNPTFPIGWGDVKPTPTGAVIRDFLAGRLPAYVETGMNVVDVDDVAEGHWLAFERGRS
                     GERYILGNANLMMRELLGELAALSGRAAPRLRLPYAVALALAHADAVVGRLVGRTRIP
                     LEGVRTAREIRFASSARAVRELGLPQTPVRTALEKAVRWFQPAVRQERSSLHGRA"
BASE COUNT          296 a          878 c          795 g          273 t
ORIGIN      
        1 gccctcgccg aacccgccgt cggggttctg gcgctcgacc agccagcgca ccgcgcgccg
       61 caccggcagc gagtccatcg gctcgccgat cgccgcgagc gccggcagca ccgcgcccgt
      121 gccgtagagg tggttgacgc cccaccggcc gaaccacgat ccgtcgggct cctgctcggc
      181 gtacaggtag tcgaccgccg cggcgaccgc cgggtggctg cgcggcacgc cgaactcgcc
      241 gagcatctcg acgacgtggg cggtgacgtc cgcggtcggc ggatcgatca tctcgccgaa
      301 gtcggcgaac gggatccgcg ccaggaggcg cgacgtgttg tccttgtcga acgccgccca
      361 cccgccggtc ttgcactgca tggccaggag ccacgcgatc gcgcgcgccc gggtcggctc
      421 gtcgagcgcg cccgcccggt gcagcccgat cagcaccagc gcggtgtcgt cgacgtcggg
      481 gtagaggtcg ttgtcgaact cgaacgacca cccgctcggc gccacgccgg ggaccgcgat
      541 ctgccagtcg cccggcacgc ggacctcctc gcggaccagc cagcgcgccg cgcgctgcac
      601 cgccgggtcg tgcgccggca cgccggactc gcgcagcgcg accagcgcca gcgcgctgtc
      661 ccacaccggc gacaggcagg cctggatgcg caggctgccg tcgctgcgcc ggatcatcca
      721 gcggccgtgc atcccgtcga gcccgcgccg gatcaccggg tgatcggcgg cgtagccgag
      781 cgcgtgcagc gccatcatcg agtacaccca cggcggctgg atcccgcccc agctgccgtc
      841 ggcctcctgg tggtcgagca gccagtgctc ggcggcgcgc cgggcgcgcg accgcagcgg
      901 gtgccacggc gcgcggtggt agcggcgcag caccgcgtcg agcccgagga acagccggtc
      961 ggccgcgtcg cggggctcga tcgcgaccgg cgcgccgatc cggagatcgg ccaggctcgc
     1021 cgacgccggc acccgccgca ccgggcggtc gttcatcagg accagcagcg gcacgatcgt
     1081 cccgcgcgcc cacgacgcga accggtagat gctcagcggc gcgcgcggcg gcagcagcat
     1141 gagctcgatc ggcatcgccg gcaggtcgtc ccagctccac tcgccgcaca gcgcgagcca
     1201 gatccgggtg aacacccggg cccgcgcgat cccgccgcgc gccgcgatga actgccgggc
     1261 gcgtgcgagg tgcgggccgc tgtcgccggt cagccggagc gcgaagtagc actcgaccgt
     1321 cgtcgacaga tcgcccgggg cgccggcgta cagcgcccag ctgccgtcgc cctgctggta
     1381 gctgcggatg tcctgggcga ccccgcgcca gatcgcggcg tcacgcgcac cgaggaagtg
     1441 cgtcatcagg atgtactccg cgtccatggt cgcgttcgac tccagctcgg cccaccagta
     1501 cccgtcctcg aactgcgcgc gccggaacca gccgtgcgcc cgcgcgatga cctgatcgac
     1561 gtcgacgtgt gcggaggccc gcgtcctctg gatctcacgc tcggccatga agcgaactcc
     1621 tttcctgcct gaccgccggc tggaaccagc gcaccgcctt ctccagcgcc gtgcgcaccg
     1681 gcgtttgtgg aagccccagc tcgcgcaccg cgcgtgcgct gctggcgaac ctgatctcgc
     1741 gcgccgtgcg cacgccctcg agcgggatcc gcgtgcgccc gaccagccgg ccgaccaccg
     1801 cgtcggcgtg ggccagcgcg agcgccaccg cgtacggcag gcgcagccgg ggcgcggcgc
     1861 ggcccgacag cgcggcgagc tcgccgagca gctcgcgcat catcaggttc gcgttgccca
     1921 ggatgtagcg ctcgcccgag cggccgcgct cgaacgccag ccagtggccc tcggccacgt
     1981 cgtcgacgtc gaccacgttc atcccggtct cgacgtaggc cggtaaccgc ccggcgagga
     2041 agtcgcggat caccgcgccg gtcggcgtcg gcttgacgtc gccccagccg atcggaaacg
     2101 tcgggttgac gatcacggcg tcgaggccgc gcgccgccgc gtcggcgacc agctgctcgg
     2161 cgcgccactt gctctgcttg tagggcccgg gcgcgccggt cgcgcgctgg cgctcgtcgg
     2221 gcagcacgcc gccgcgcatg cc
//
LOCUS       DASVOR010002349         4013 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_171838,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002349 DASVOR010000000
VERSION     DASVOR010002349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171838"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1496)
                     /locus_tag="VF516_43240"
     CDS             complement(<1..1496)
                     /locus_tag="VF516_43240"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8114645.1"
                     /translation="MIRPALLLAAVAACSSPPPRAAEAPRSSNDPRLVVLLVVDQWPE
                     WSFEAKRTALTGGFARLLREGEWHVGQHPSAATLTAAGHALLGTGEPPYRSGILANEW
                     WHRDVARQLKAIEAEDGAMSVKWLRAPGLADALAAAHTGGRAVSVSLKDRAAILPLGH
                     AGTAIWYDAHQVAWTSLTPPPWLAAWNAAQPIAAHLHDVWTPLPETPRMASVPDDAPG
                     EIGIMGLGPTFPHALDATRSPAAAIFATPLGNDLVFDTATAAIEREHLGAGSRPDLLI
                     LSLSAHDYIGHGWGQESWESWDTALRLDRRLDRFLADLDARVGAGRWAMIATSDHGAS
                     PMPESLHGGRITYEQVKDAANRAAIAELGPGEWIADARFPTVYLSAAALAQKPRDLAV
                     AMTKIVYALRAFPGIERAEKSSEFWGHCDSRTGDAFLICITLDPERSGEIFYLPARGW
                     VLEEGSEAVATGHGSLQPYDRQVPVIVLPPGRAAHAALTGPDAAAIAM"
     gene            1809..2399
                     /locus_tag="VF516_43245"
     CDS             1809..2399
                     /locus_tag="VF516_43245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEX8114646.1"
                     /translation="MRISHIVTALAWMVACTSATSPLAASPSDVRVYPSPSESAITEG
                     ETVLAAEPDAVYRAVTDYPRWTTMFPDIRRVIITSQAGGHERVTFVHADGNRDNVHFH
                     NVPAARMVWFEDTGGRAEVWVEILLLPGERAGTTRVHSRLYADVHGWASLFVTDGKLR
                     SMREQRIREDLTHLRAYFARDVAVQVPDRPAIDGGM"
     gene            2449..2700
                     /locus_tag="VF516_43250"
     CDS             2449..2700
                     /locus_tag="VF516_43250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114647.1"
                     /translation="MATRASADLVADLATLARLHAALDSVVETEQVIAAIHDSSASRA
                     AAVVRVSALLGEARELLSGLLVRPDRRHAAVGSPDSLAG"
     gene            2900..>4013
                     /locus_tag="VF516_43255"
     CDS             2900..>4013
                     /locus_tag="VF516_43255"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114648.1"
                     /translation="MECPGTEMFELMFGGQLTPEQQGPLADHAATCPACHAVVEAMVT
                     QHGPAHAEPRPLTVGSMIGGRFRIDQVLGAGGMGIVVAATHTELGNRVAIKFMRDEKL
                     KSAEAVERFIREARAVVKLRTEHVCRVYDVARLDTGAPYIVMELLQGVDLARTIASRP
                     LPATIAVEYVMQAAVALAEAHAAGIVHRDLKPANLFVTRRHDGGPLVKVLDFGIAKAL
                     TESDSRLTRTRAMLGSPGYMSPEQLDSPRDVDGRSDIWALGVTLYQLLSGRLPFWGKN
                     PTEIAVKIAAEDPPPLEVDPPLAAVVRRCLEKSPGDRYQDVAALAADLKPFGGPRARK
                     IVAAIAKTTSAPILPTPGELPAVPAHVATAATAATGA"
BASE COUNT          545 a         1435 c         1485 g          548 t
ORIGIN      
        1 tgcatcgcga tcgcggccgc gtcggggccg gtgagcgcgg cgtgcgcggc gcggccgggc
       61 ggcagcacga tcaccgggac ctggcggtcg tacggctgca gcgagccgtg gccggtcgcg
      121 accgcctcgc tgccctcctc gagcacccag cccctggccg gcaggtagaa gatctcgccc
      181 gagcgctcgg gatcgagggt gatgcagatc aggaacgcgt cgccggtccg gctgtcgcag
      241 tgcccccaga actcggagct cttctcggcg cgctcgatcc cggggaacgc gcgcagcgcg
      301 tagacgatct tggtcatcgc caccgcgagg tcccggggct tctgggccag cgccgcggcc
      361 gacaggtaca cggtggggaa ccgcgcgtcc gcgatccact caccggggcc gagctcggcg
      421 atcgccgcgc ggttggcggc gtccttgacc tgctcgtacg tgatccgccc accgtgcagc
      481 gactcgggca tcgggctcgc gccatgatcg ctggtcgcga tcatcgccca ccggccggcg
      541 ccgaccctgg cgtcgagatc ggcgaggaag cggtcgagcc ggcgatcgag ccgcagcgcg
      601 gtgtcccacg actcccacga ctcctggccc cagccgtggc cgatgtagtc gtgggcggac
      661 aggctgagga tcaggaggtc ggggcgggag ccggcgccga ggtgctcgcg ctcgatcgcg
      721 gcggtcgcgg tgtcgaacac caggtcgttg ccgagcggcg tcgcgaagat cgcggcggcg
      781 gggctccggg tcgcgtcgag cgcgtggggg aacgtcggcc cgaggcccat gatgccgatc
      841 tcgccgggcg cgtcgtcggg cacgctcgcc atccgcggcg tctcgggcag cggcgtccac
      901 acgtcgtgga ggtgcgccgc gatcggctgg gcggcgttcc acgcggcgag ccacggcggc
      961 ggcgtcaggc tggtccacgc gacctgatgg gcgtcgtacc agatcgccgt gccggcgtgg
     1021 ccgagcggca ggatcgcggc gcggtccttg aggctgacgc tcaccgcccg gccgccggtg
     1081 tgggcggcgg cgagcgcgtc ggccagtccg ggcgcgcgca gccacttcac cgacatcgcg
     1141 ccgtcctcgg cctcgatcgc cttgagctgg cgcgcgacgt cgcggtgcca ccactcgttg
     1201 gcgaggatcc ccgagcggta cggcggttcg ccggtgccga gcagcgcgtg gccggcggcg
     1261 gtcagggtcg ccgccgacgg atgctggccg acatgccact cgccctcgcg cagcagccgg
     1321 gcgaagccgc cggtcagcgc ggtgcgcttg gcctcgaacg accactccgg ccactgatcg
     1381 acgaccagca gcacgaccag gcgcggatcg ttgctcgacc ggggcgcctc cgcggcgcgc
     1441 ggcggtgggc tggagcaggc agcgacggcg gcgaggagca gggcggggcg gatcacgttt
     1501 gcgccacaat accgcggaca cgcgcgctcg ccggccggcc gttgccatgg catgcagcgt
     1561 catcctcgcg ccgcacccgc actgtgctat cccgcacgaa cggcgcactc ccgcagccca
     1621 tgcgcgaccc gcgacaaccg tgtcgcgacc tgtcggaatt tccctgtatg ccgctccggc
     1681 aggtgccggc gaggtgccgg gtactgcctg caggtcctcg aacttgcgcg gtgaccccat
     1741 tggcgcgatc gttgctcggg caacgctaca gcgcacagcc cgctccaacc tgggccgtgc
     1801 gaaccgtcat gcggatcagt cacatcgtca cagcgctcgc gtggatggtg gcatgcacgt
     1861 cggcgacctc gccgctcgcg gcgtcgccgt ccgacgtccg ggtgtatccc agccccagcg
     1921 agagcgcgat caccgagggc gagaccgtgc tcgcggcgga gcccgacgcc gtgtaccgcg
     1981 cggtgaccga ctacccgcgg tggacgacca tgttccccga catccggcgg gtcatcatca
     2041 ccagccaggc cggcgggcat gaacgcgtga cattcgtgca cgccgacggc aaccgcgaca
     2101 acgtgcactt ccacaacgtg cccgccgcgc ggatggtgtg gttcgaggac accggcgggc
     2161 gcgccgaggt ctgggtcgag atcctgctcc tgcccggcga gcgagccggg acgacgcgcg
     2221 tgcactcgcg gctgtacgcc gacgtccacg gctgggcgtc gctgttcgtg accgacggca
     2281 agctgcgctc gatgcgcgag cagcgcatcc gcgaggacct gacgcacctg cgcgcgtact
     2341 tcgcgcgtga cgtcgcggtg caggtacccg accgtccggc gatcgacggc gggatgtgag
     2401 cggtgggcgc cgcgctgctg gcgcgccgct tgctctgctg ttccgtcgat ggctacgcga
     2461 gcttccgcag acctggtcgc cgacctggcg acgctggcac gacttcacgc ggcgctggat
     2521 tcggtcgtcg agaccgagca ggtgatcgca gcgatccacg atagttcggc gagccgcgcg
     2581 gccgcggtgg tccgggtcag cgcgctgctc ggcgaggcgc gcgagctgct gagcgggctg
     2641 ctcgtccgtc cggaccgccg acacgccgcc gtcggatcgc cggacagtct ggcaggttag
     2701 ccgcgcggct cctgaccgat cgtcacggca ggcgattcca aaccattgcg gtgggttgcg
     2761 cgagcgccgc agggcgccgc gcacaaatcg tgcgacaaat cgcggggacc ggctccttga
     2821 tgccacgagc catcgctcgt ccgggagggg ccatgccggt cgctacactt ggtcggaaag
     2881 cgagaatact ctgttgaggg tggagtgtcc gggcaccgag atgttcgagc tgatgttcgg
     2941 ggggcagctc accccggagc agcaggggcc gctcgccgac cacgcggcga cctgcccggc
     3001 gtgccacgcg gtcgtcgagg cgatggtgac ccagcacggc ccggcgcatg ccgagccgcg
     3061 gccgctgacg gtcggctcga tgatcggcgg ccggtttcgc atcgaccagg tgctcggcgc
     3121 gggcggcatg ggcatcgtcg tcgccgcgac ccacaccgag ctcggcaacc gcgtcgcgat
     3181 caagttcatg cgcgacgaga agctcaagag cgccgaggcg gtcgagcgct tcatccgcga
     3241 ggcccgcgcg gtggtcaagc tgcgcaccga gcacgtgtgc cgggtgtacg acgtcgcgcg
     3301 gctcgacacc ggcgcgccgt acatcgtgat ggagctgctg cagggcgtcg atctcgcgcg
     3361 cacgatcgcg tcgcggccgc tgccggcgac gatcgcggtc gagtacgtga tgcaggccgc
     3421 ggtcgcgctc gccgaggccc acgccgccgg catcgtgcac cgcgatctca agccggcgaa
     3481 cctgttcgtg acccgtcgcc acgacggcgg cccgctggtc aaggtgctcg acttcgggat
     3541 cgccaaggcg ctcaccgaga gcgactcgcg gctgacccgg acccgcgcga tgctcggctc
     3601 gccgggctac atgtcgcccg agcagctcga ctcgccgcgc gacgtcgacg gccgctccga
     3661 catctgggcg ctcggcgtca cgctgtacca gctgctgtcc gggcggctgc cgttctgggg
     3721 caagaacccg accgagatcg cggtcaagat cgccgccgag gacccgccgc cgctggaggt
     3781 cgatccaccg ctcgcggcgg tcgtgcggcg ctgcctcgag aagtcgcccg gcgaccgcta
     3841 ccaggacgtc gcggcgctcg ccgccgatct caagccgttc ggcgggccgc gggcgcgcaa
     3901 gatcgtcgcg gcgatcgcga agacgacgag cgcgccgatc ctgccgacgc cgggggagct
     3961 gccggcggtg ccggcgcacg tcgcgaccgc ggccaccgca gcgaccgggg cca
//
LOCUS       DASVOR010002350         4691 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_27338,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002350 DASVOR010000000
VERSION     DASVOR010002350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27338"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VF516_43260"
     CDS             <1..255
                     /locus_tag="VF516_43260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114649.1"
                     /translation="SAAGLVQRMHAAFAPPGQALPAWEAIVRLASICAPVTAAGAGKL
                     SFTHAREVFKDMTQTVPAWSALTWAREARPLALRFAGSRG"
     gene            257..1492
                     /locus_tag="VF516_43265"
     CDS             257..1492
                     /locus_tag="VF516_43265"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEX8114650.1"
                     /translation="MSLLYAILDFPAVKWAFLVLALVMPLASLLTWAERRQSAMMQDR
                     LGPNRANIGPIRLKGILHFVADALKMIFKEDFIPANVHKGLFTLAPILALAPVLIAFA
                     IIPFGPTIYPHQVTEVINPTELVTNPGSPAFADAIRLQIFQIDFGLIFYFAVLTIANY
                     GGTIAGWASYNKWALLGGLRSSSQMMSYEVAMGLGLMPIFLAFGTLEPGKLVLAQGPL
                     PWNWGVIGWSQFATAAASIFGAASLVLAFLLFFTAAIAETKRAPFDIPEGEPEIIGYF
                     VEYSGMRWGMFFLAEFIEIVFISAVIATVFFGGWQVPFLDPDGFRIGGYVDPNHVSTS
                     GWVLHLPHGIVVLIQFLGFGAKLVLLSVFQLLVRWTLPRFRADQLMNLGWKLLLPLSL
                     INIMAIALIKLFAMAVTGS"
     gene            1496..2215
                     /locus_tag="VF516_43270"
     CDS             1496..2215
                     /locus_tag="VF516_43270"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEX8114651.1"
                     /translation="MSAEHDEHAHGHGEHAGAAAGPVVLGVSDRHPRVRTIRVVRPAA
                     EETTFLTAAVKGLGITLKHFARNLFLPLRSAAARGKAGAPTDEAWRNEIETIQYPDEK
                     VTYPERFRGLHRLTLRDDGAVRCVACMCCPTVCPAHCITIIPEESESKGIEKRPAVFE
                     IDELRCVVCGLCVEACPCDAIRMDTGQHAQPVEDRARAIETKESMMGRGVRSIARQGG
                     EGPNWREHEPGSQAHRNDADH"
     gene            complement(2771..3748)
                     /locus_tag="VF516_43275"
     CDS             complement(2771..3748)
                     /locus_tag="VF516_43275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX8114652.1"
                     /translation="MAAVVTPAASVIAALVLIALLVRNPAPFARLRLVVLVPWWLAGL
                     VLRWIPGLWWLPQLMRWIPPMLLLRAPEIFVDQWRAIDAETVRDPADAGRANLQVLVL
                     LVTVAASLTLQEYLGGHDSYERLFPADGSRYWELRSFAWWSGWRVFGYVGIPMIVLAC
                     LPGQRIRDYHVSLRGFFHHLWIYAALFALVLPAVIYASTTVAFRETYPFYRIANRSYT
                     DLALWELLYAAQFLSLEFFFRGFLLHGLRRALGANAIYVMLVPYCMIHYGKPLPETLG
                     AIGAGLILGTLAMRTRSIWGGVLIHVGVATTMDVLALRGCPPFGGAPCH"
     gene            complement(3749..>4691)
                     /locus_tag="VF516_43280"
     CDS             complement(3749..>4691)
                     /locus_tag="VF516_43280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEX8114653.1"
                     /translation="AEIDDQVLLKSDGLPTYHLANVVDDHLMEISHVIRAEEWISSTQ
                     KHVLLYRAFGWEAPRFYHMPLLRNADKDKTKISKRKQPVSVNYYRDIGILPEALLNFL
                     GTMGWSFGGDREKFTLQEMIEVFSWDRISLGGPVFNLDKLTWLNEKYLHELSIDELAD
                     RVIAWRLNKDYLRTVLPLVQKRIRKLDELVPATEFFFSGDLDLQPVLGELTVPDVAPA
                     DVSRALLAYVERFEARVGWTKQMLEDEANAWCAELGWKTKHAFMLLRVAVTGRTATPP
                     LFETMAALGKEITRRRLRRAAEAIGKTKAPEAAAKAK"
BASE COUNT          725 a         1568 c         1643 g          755 t
ORIGIN      
        1 agcgccgcgg ggctcgtgca gcgcatgcac gcggcgtttg cgccgcccgg ccaggcgctg
       61 ccggcgtggg aggcgatcgt ccggctcgcg tcgatctgcg ctccggtcac cgccgccggc
      121 gccggcaagc tcagcttcac gcacgcgcgt gaggtgttca aggacatgac gcagaccgtg
      181 cccgcgtgga gcgcgctgac ctgggcacgc gaagcccggc cgctcgcgct gcggttcgcc
      241 gggagccgag ggtgacatga gcctgctcta cgcgatcctg gatttccccg ccgtcaagtg
      301 ggcgttcctc gtcctcgccc tggtcatgcc gctggcctcg ctgctgacct gggccgagcg
      361 ccggcagagc gcgatgatgc aggaccggct cggccccaac cgcgcgaaca tcgggccgat
      421 ccggctcaag ggcatcctgc atttcgtcgc cgacgccttg aagatgatct tcaaggagga
      481 cttcatcccg gccaacgtgc acaaggggct gttcacgctc gccccgatcc tcgcgctcgc
      541 gccggtgctg atcgcgttcg cgatcatccc gttcgggccg acgatctacc cgcaccaggt
      601 gaccgaggtc atcaacccga ccgagctggt gaccaacccg ggctcgccgg cgttcgccga
      661 cgcgatccgg ctgcagatct tccagatcga cttcggcctg atcttctact tcgcggtcct
      721 caccatcgcc aactacggcg gcacgatcgc cgggtgggcg agctacaaca agtgggcgct
      781 gctcggcggg ctgcgcagct cgtcgcagat gatgtcgtac gaggtcgcga tgggcctggg
      841 gctcatgccg atcttcctcg cgttcggcac gctggagccc ggcaagctgg tgctcgcgca
      901 ggggccgctg ccgtggaact ggggcgtgat cggctggagc cagttcgcca cggccgcggc
      961 atcgatcttc ggcgccgcct cgctggtgct ggcgttcctc ctgttcttca ccgccgcgat
     1021 cgccgagacc aagcgcgcgc cgttcgacat ccccgagggc gagcccgaga tcatcgggta
     1081 cttcgtcgag tactccggca tgcgctgggg gatgttcttc ctcgccgagt tcatcgagat
     1141 cgtgttcatc tcggcggtga tcgcgaccgt gttcttcggc ggctggcagg tgccgttcct
     1201 cgacccggac gggttccgga tcggcggcta cgtcgacccg aaccacgtgt cgaccagcgg
     1261 ctgggtgttg cacctgccgc acggcatcgt cgtgctgatc cagttcctcg ggttcggcgc
     1321 caagctggtc ctcttgtcgg tgttccagct gctggtccgc tggaccttgc cgcggttccg
     1381 cgccgaccag ctgatgaacc tcggctggaa gctcctgttg ccgctgtcgc tcatcaacat
     1441 catggcgatc gcgctgatca agctgttcgc catggccgtg acggggtcct gaacgatgtc
     1501 agctgaacat gacgagcatg cgcatggtca cggcgagcac gccggtgcgg ccgccgggcc
     1561 ggtggtcctg ggcgtctcgg accgccaccc ccgggtgcgg acgatccggg tggtccggcc
     1621 ggcggccgag gagaccacct tcctgaccgc ggccgtcaag ggcctgggca tcacgctcaa
     1681 gcacttcgcg cgcaacctgt tcctgccgct gcgctcggcc gcggcgcgtg gcaaggccgg
     1741 ggcaccgacc gacgaggcgt ggcgcaacga gatcgagacc atccagtacc cggatgagaa
     1801 ggtcacgtat ccggagcggt tccgcggcct gcaccgcctg accctgcgcg acgacggcgc
     1861 ggtgcgttgc gtcgcgtgca tgtgttgccc gaccgtgtgc cctgcgcact gcatcacgat
     1921 catccccgag gagagcgaga gcaaggggat cgagaagcgc ccggcggtgt tcgagatcga
     1981 cgagctgcgt tgcgtggtct gcggtctgtg cgtcgaggcc tgcccgtgcg acgcgatccg
     2041 gatggacacc ggccagcacg cccagccggt cgaggaccgc gcccgcgcga tcgagaccaa
     2101 ggaatcgatg atggggcgcg gcgtacgttc gatcgcgcgg cagggcggcg aaggaccgaa
     2161 ctggcgagag cacgagcccg gctcgcaggc gcatcgcaac gacgccgatc actgatctct
     2221 caccgacgag atttgccgca ttgcaggttg cggtggcgat gcgctccggc cgcgcgcgat
     2281 gcgcggcgca ggcgcttttt ccagttgacg gatccagact cgatccttag tctgaaattc
     2341 gagcaccggc ggtctgatgc gagacccggg gcgtatctgt ctcctacaat agctatccag
     2401 ggatctgcct ctgttccggg agtgcacgcc cgctcgtcgg gaagcccgaa ggagtacgtg
     2461 gagcccacct acgcacatgc gtaggggata gaaaccccca cggtcggacg gtcgtgctca
     2521 ttttgggcgc agcggttgaa tcgccgctgc gccctccctg tttttcggtg tcggcggtcg
     2581 cggcagcctc ggcagcccgc ggcgttgcac ttgaagtgca aaaagccatt catgtgcggc
     2641 gctggagatg agcgcgtgcg cctgtgcgcg gtgcattcat tgccacggtc ccccgagggg
     2701 ggcctccctt cgaaggcggc gaagacgggc cctccgcggg gggcgtgtgc tctggcgcgc
     2761 acgggtccgt tcagtggcag ggggcgccgc cgaacggcgg gcagccgcgc agggcgagca
     2821 cgtccatcgt ggtggcgacg ccgacgtgga tcagcacgcc gccccagatc gagcgggtgc
     2881 gcatggcgag cgtgccgagg atcaagccgg cgccgatcgc gccgagcgtc tcgggcaacg
     2941 gcttgccgta gtggatcatg cagtacggca cgagcatcac gtagatcgcg ttggcgccga
     3001 gcgcgcggcg caggccgtgg agcaggaagc cgcggaagaa gaactcgagc gacaggaact
     3061 gcgcggcgta caggagctcc cacagcgcga ggtcggtgta ggaccggttg gcgatccggt
     3121 agaacgggta ggtctcgcgg aacgccaccg tggtcgacgc gtagatcacg gccggcaaca
     3181 ccagcgcgaa cagcgcggcg tagatccaca ggtggtggaa gaacccgcgc agcgacacgt
     3241 ggtagtcgcg gatccgctgg ccgggcaggc aggcgagcac gatcatcggg atgccgacgt
     3301 agccgaacac gcgccagccc gaccaccagg cgaagctgcg cagctcccag tagcggctgc
     3361 cgtccgccgg gaacagccgc tcgtagctgt cgtggccgcc gaggtactcc tgcagcgtga
     3421 gcgaggcggc gaccgtgacc aggaggacca gcacctggag gttggctcgc ccggcgtcgg
     3481 cggggtcgcg cacggtctcg gcgtcgatcg cccgccactg atcgacgaag atctccggcg
     3541 cgcggagcag cagcatcggc gggatccacc gcatcagctg cgggagccac cacagcccgg
     3601 ggatccagcg cagcacgagc ccggcgagcc accacgggac cagcacgacc agccgcagcc
     3661 tggcgaacgg cgcgggattc cggaccagca acgcgatcag gacgagcgcc gcgatgacgc
     3721 tcgcggcagg ggtgacgacc gccgccacct acttggcctt ggcggcggcc tctggcgcct
     3781 tggtcttgcc gatggcctcg gccgcgcggc gcagccggcg ccgcgtgatc tccttgccga
     3841 gcgcggccat ggtctcgaac agcggcgggg tggcggtgcg gccggtgacc gcgacccgca
     3901 gcagcatgaa cgcgtgcttg gtcttccagc ccagctcggc gcaccacgcg ttcgcctcgt
     3961 cctcgagcat ctgcttggtc cagccgaccc gggcctcgaa gcgctcgacg taggcgagca
     4021 gcgcgcgaga gacatccgcc ggtgcgacgt cggggacggt cagctcgccg agcaccggct
     4081 ggaggtcgag gtcgcccgag aagaagaact cggtcgccgg gacgagctcg tcgagcttgc
     4141 ggatccgctt ctggaccagc ggcagcacgg tcctgaggta gtccttgttg agccgccacg
     4201 cgatcacgcg gtcggcgagc tcgtcgatcg acagctcgtg caggtacttc tcgttgagcc
     4261 aggtcagctt gtcgaggttg aacaccgggc cgccgagcga gatccggtcc cacgagaaca
     4321 cctcgatcat ctcctgcagc gtgaacttct cgcggtcgcc gccgaacgac cagcccatgg
     4381 tgccgaggaa gttgagcagc gcctcgggca ggatgccgat gtcgcggtag tagttgaccg
     4441 acaccggctg cttgcgcttg ctgatcttgg tcttgtcctt gtcggcgttg cgcagcagcg
     4501 gcatgtgata gaacctcggc gcctcccagc cgaacgcccg gtacagcagg acgtgcttct
     4561 gggtcgacga gatccactcc tcggcgcgga tcacgtggct gatctccatc aggtgatcgt
     4621 cgaccacgtt ggcgaggtgg taggtcggca ggccgtcgga cttgagcagc acctggtcgt
     4681 cgatctcggc g
//
LOCUS       DASVOR010002351         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_404746,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002351 DASVOR010000000
VERSION     DASVOR010002351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404746"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="VF516_43285"
     CDS             <1..1082
                     /locus_tag="VF516_43285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114654.1"
                     /translation="AQPATPAPAPATPPPAVPSPATAAGSRAKPAPMKTDEKAGANEA
                     LQRNAGYKNVSKADEDCALRAFHQGNDLLNNGLFPQAVARYREALRCWDHPGIHYNLA
                     LALINLDQPIEVYEQLNEAIKYGADPLDEGKFEHAREYLKLVSGLLADVEVSCDKPGA
                     KVAVDGKEVFVAPGSFKAKVRVGKHTFYGEKEGYNARVTAPFIGPGETFRIELKLYTA
                     EELTRYRRRWEKTWMPYAVIGGGALVGVIGGVLELSASSSYKDFNSAVAKCNTALMGC
                     AASTPGLIDMRNSGDTKRTLGYVGYGVAGAAIATGALLAFLNRRTPYQIRPEDLEEDK
                     DKAHAVAIAPVVSPGMTGAMVQGHF"
     gene            1095..>2121
                     /locus_tag="VF516_43290"
     CDS             1095..>2121
                     /locus_tag="VF516_43290"
                     /inference="COORDINATES: protein motif:HMM:TIGR02232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4215 domain-containing protein"
                     /protein_id="HEX8114655.1"
                     /translation="MDSITRHFGLLCLGALVAAITACASNSTSQCEATGVLCPSGTHC
                     AAAQPICISDTIRCGDAHLDADEECDDGNNIDGDGCSHLCTIEICGNHRIDPHEQCDD
                     GNTADGDGCSHNCTVEVCGNGVKDFGEVCDDGNTKDGDGCSANCKSREICGNGTVDKA
                     VGEVCDDGNTVGGDHCSADCRSGEGCGNGIVDPGEECDDGNIDNNDDCRNDCVVNRCG
                     DGVLDMNGTHTEECESAIEQTNGSKLLTSTESASCNNDCTIPKCGDGKVNHSFTPSGA
                     AGPEQCDVVDATGHSLNGDNADCTAHCQINACGDGLHNTQGPNHAEACDDGNKIDSDG
                     CSNACTLP"
BASE COUNT          395 a          745 c          718 g          263 t
ORIGIN      
        1 ccgcgcagcc ggcgaccccg gcgcccgcgc cggccacgcc gcctcccgcc gtcccgtcgc
       61 cggccacggc cgccggctcg cgcgccaagc cggcgccgat gaagaccgac gagaaggccg
      121 gcgccaacga ggcgctgcag cgcaacgccg gctacaagaa cgtgtccaag gccgacgagg
      181 actgcgcgct gcgcgcgttc catcaaggca acgacctgct caacaacggc ctgttcccgc
      241 aggcggtcgc gcgctaccgc gaggcgctgc gctgctggga ccaccccggc atccattaca
      301 acctcgcgct cgcgctgatc aacctcgacc agccgatcga ggtctacgag cagctcaacg
      361 aggcgatcaa gtacggcgcg gatccgctcg acgagggcaa gttcgagcac gccagggagt
      421 acctcaagct ggtctccggg ctgctcgccg acgtcgaggt gtcgtgcgac aagccgggcg
      481 ccaaggtcgc ggtcgacggc aaggaggtgt tcgtcgcgcc gggcagcttc aaggccaagg
      541 tccgggtcgg caagcacacg ttctacggcg agaaggaggg ctacaacgcc cgggtcaccg
      601 cgccgttcat cggccccggc gagaccttcc ggatcgagct caagctgtac accgccgagg
      661 agctgacccg gtaccgccgc cggtgggaga agacctggat gccgtacgcc gtgatcggcg
      721 gcggcgcgct ggtcggcgtg atcggcggcg tgctcgagct gtcggcgagc tcgagctaca
      781 aggacttcaa ctccgcggtc gccaagtgca acaccgccct gatgggctgc gcggcgagca
      841 cgcccggcct gatcgacatg cgcaacagcg gcgacaccaa gcggacgctc ggctatgtcg
      901 gctacggcgt cgcgggggcc gcgatcgcga ccggcgcctt gctggcgttc ctcaaccgcc
      961 gcacgccgta ccagatccgc cccgaggatc tggaagaaga caaggacaag gcgcacgccg
     1021 tggcgatcgc gccggtcgtg tctcccggga tgaccggggc gatggttcag ggccacttct
     1081 aggagacttc agtcatggac tccattaccc gacacttcgg actcctgtgt ctcggcgcgc
     1141 tcgtcgcggc gatcaccgcg tgtgcctcga actcgacctc gcagtgcgag gcgaccggcg
     1201 tcctgtgccc gtccgggacg cactgcgccg ccgcgcagcc gatctgcatc tcggacacga
     1261 tccggtgcgg cgacgcccac ctcgacgccg acgaggagtg cgacgacggc aacaacatcg
     1321 acggcgacgg ctgctcgcac ctgtgcacga tcgagatctg cggcaaccac cggatcgatc
     1381 cgcacgagca gtgcgacgac ggcaacaccg ccgacggtga cggctgctcg cacaattgca
     1441 cggtcgaggt ctgcggcaac ggcgtcaagg acttcggcga ggtgtgcgac gacggcaaca
     1501 ccaaggacgg cgacggctgc tcggcgaact gcaagtcgcg ggagatctgc ggcaacggca
     1561 ccgtcgacaa ggcggtcggc gaggtgtgcg acgacggcaa cacggtcggc ggcgaccatt
     1621 gcagcgcgga ctgccggtcg ggcgagggct gcggcaacgg catcgtcgat cccggcgagg
     1681 agtgcgacga tggcaatatc gataacaacg acgactgccg caacgactgc gtggtcaacc
     1741 gctgcggcga cggcgtgctc gacatgaacg gcacgcacac ggaggagtgc gagtcggcca
     1801 tcgagcagac gaacggctcg aagctgctga cctccaccga gtcggcgagc tgcaacaacg
     1861 actgcacgat cccgaaatgc ggcgacggca aggtcaacca cagcttcacc ccgtccggcg
     1921 cggccgggcc cgagcagtgc gacgtcgtcg acgccaccgg ccattcgctc aacggcgaca
     1981 acgcggactg caccgcgcac tgccagatca acgcgtgcgg cgacgggctg cacaacaccc
     2041 agggccccaa ccacgccgag gcgtgtgacg acggcaacaa gatcgactcc gacggctgct
     2101 cgaacgcgtg cacgctcccg c
//
LOCUS       DASVOR010002352         1663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_404754,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002352 DASVOR010000000
VERSION     DASVOR010002352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404754"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            653..841
                     /locus_tag="VF516_43295"
     CDS             653..841
                     /locus_tag="VF516_43295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114656.1"
                     /translation="MKKASNKHLVLKRDTLKALTELPNTAYRHVVGGAAPRQQPTGCH
                     NDCAGSAQTAGGPSQQDQ"
     gene            1365..>1663
                     /locus_tag="VF516_43300"
     CDS             1365..>1663
                     /locus_tag="VF516_43300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114657.1"
                     /translation="MSQSSLNNSPHSGFDVSTVDYYSKGDRRSSGAAEDPRDRTESLG
                     DGDRSELQGRVLGDFLLGEVLGQGGCGVVYRAEQRTLGRPAVVKVVHRAMATRLD"
BASE COUNT          274 a          564 c          573 g          252 t
ORIGIN      
        1 gccgtttctg cgtcttcagc ttctgcgtcg gggcgcgccg gaggtccgga tcctgcggag
       61 ctttctgcga catggtcatc tcctggattg acgtggatgc gccgacaccc atgtgcaagc
      121 cccgagcccc ggcggagctc gatcgacgac gtccggtgga cagatgtact cgcgtgaccg
      181 gcacggcgcg atccgtcgcg atcgtcccgg gttggcgagg cccggaccgc ggccggacca
      241 ccggccggtg tcgccgcaag gcaggcgcgg ccgtcggcgc gcgcgctgga catgcacaac
      301 cttccactgg acatgttcag cgcgcgggct ggacacccgc ggctggagtc gctgtggctc
      361 gccggtgacc ggtatgcggt gattgcaaga cttgcacgac ggcaagactt gcaatcggcg
      421 cgctcgcaag tcttgcttcc gctcggcggg atacggcgcg acctcgatcg aacagaacca
      481 cgcgcacgtc gcgcgataca cagatcccgg cacacaggag gggatggccg ggcgtgggta
      541 cgcaggttgc ttcctgctct cctcggtcac gccgagctcg atctcgatac acaggttcat
      601 gcgacgcaat ggcgcgtgag ctgccggcgc ccagaacaag gagagacatt tcatgaagaa
      661 ggcttcgaac aagcatctcg tcctcaagcg cgatacgctg aaagcgttga cagagctgcc
      721 gaacactgca taccgacatg tggtcggcgg cgccgccccc cggcagcagc cgacggggtg
      781 ccacaacgac tgtgctggtt cggcgcaaac ggccggcggc ccttcgcagc aagatcagtg
      841 atcaccgacg atgcgtcctg cggcgatgcc catcaccgtg gcatcgacgt cgcgacggtc
      901 ggcgacgacc gcaagggcga ccgccggagc ccgggcaccg cgaaggatcc gcgcgacaga
      961 acacagagcc tagggcgtgg agaccggtcc ggatggcagg ttccggtctc cgcccgctgg
     1021 tgaacgagtc gcttctcgcg ggcgagcagc gaccgaccac cgtcgttgcc ggggaaatgc
     1081 tccggctgct ggtgtccagt ccgcgcgctg aacatgtcca gtacatggtt gtgcgtgtcc
     1141 agcgcctgcc gcgccggtcg cggccgcctt gcggcgacac cggccggcgg cccgaccgcg
     1201 ctccgggcct cgccaacccg ggacgatcgc gacggatcac gccgtgccgg tcacgcgatg
     1261 tcatctgtcc accggacgtc gcggatcgag ctccgcacgg gctcgtggct tgcacgagag
     1321 gcagccgtcg cgggcggaac acgctgcgcg catcgcacac cgccatgtcg cagagcagcc
     1381 tcaacaacag tcctcacagt ggcttcgatg tatcgacggt cgactactac agcaagggcg
     1441 accgccggag ctcgggcgcc gcggaggatc cccgcgaccg gacggagagc ctcggcgacg
     1501 gggaccggtc cgagttgcag ggccgcgtcc tcggcgactt cctgctcggc gaggtgcttg
     1561 gccagggcgg ctgcggtgtc gtctaccggg ccgagcaacg cacgctcggc cgtcccgcgg
     1621 tcgtcaaggt cgttcatcgc gccatggcga cgcggctgga tat
//
LOCUS       DASVOR010002353         3369 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_142586,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002353 DASVOR010000000
VERSION     DASVOR010002353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3369
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142586"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3369)
                     /locus_tag="VF516_43305"
     CDS             complement(<1..>3369)
                     /locus_tag="VF516_43305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEX8114658.1"
                     /translation="VLDRTGAERFARAYAAVLASAVADPTLAIDRLSAVDAAEAAQLR
                     ALSGLPAMRSRRFVSAVELILRAGAAHPDAIAVECAGRTLTYRELVARARTAAATLRD
                     RLTRARGAGSPDGSIGGAKDLPGGIPRDTLIGLLLERSIDLPVAMIAVWMLGAAYVPL
                     DPKYPRAVLEHAVHDSGLALLVTNLPDAAGFDGVPSCLLAEPAGATLDDIVALDPHAT
                     AYVIYTSGSTGKPKGVIVEHDQFSNFCAGMDQVVGGGRGDRWLAVTSSSFDISGLELV
                     WTLTRGYHVIVARDARDALAAMDRRTAAPPTHLQCTPSLARMLLADEAGRRLLGDLDR
                     LLVGGEALDRTLATRLDRVVRGSVINMYGPTETTIWSSCWAVGDGEVSLGAPIANTAL
                     YVLDAHGAEVPRGMLGELWIGGDGVARGYLRRPDLTAARFVPDPRRGDGFRMYRTGDL
                     VRYRDDDSLEFCGRADHQIKLRGHRIELGEIDAVATAFAGVAECAAVVGTDVPDDPQL
                     CLYWVAASAEVDPAALEAHLSRELPPHMVPSRLYQLAGLPHTPNGKIDRGALLAERPM
                     GTPAPRAAATAGEAGAGLRALLTEVWREVLGVAEVPPDRGFFELGATSMTAVVAHRAI
                     VARLGRELPLSALFGYPTIARLAAYLGDAAETTPVARRARRDPDDAIAIVGMACRVPG
                     ADDLAEYWRNLVAGLDTVARFSRDELRAAGIPTAALADPGYVGAKGIVPTADRFDAGF
                     FDYSPGDAELIDPQHRVFLECAWEALEDAGLVPDRFAGRVAVFGGVGQWGERPAAAAD
                     LASYYRNILGGRSDFFATRVAHKLDLTGPAVNVQTACSTSLVAIHMARQAILRGEADA
                     AIAGGVTLTFPIKHGYPYQPGLVVSPDGRCRPFDAGASGTVISNGAGVVVLRRVADAL
                     AGGDRIYAVLRGSAINNDGAAKVSFAAPSVRGQAEVIAAAYADAGITPDTISLVEAHG
                     SGTALGDPIEVQALQQVFRNVGRAEPCALGSVKSNLGHLDTAAGVAGLIKAALCLRHA
                     ELVPSVHFTAPNPELRLESDLFEVVREHRPWRAPSGAPRRAGVSSFGMGGTNAHAVLE
                     EAPAIARPATRPLATVPIVISARDERALRAQAAR"
BASE COUNT          474 a         1194 c         1287 g          414 t
ORIGIN      
        1 agcgcgccgc ctgcgcccgg agcgcccgct cgtcacgcgc ggagatcacg atcggcaccg
       61 tggcgagcgg ccgggtcgcc gggcgcgcga tagcaggcgc ttcttcgagg acggcgtgcg
      121 cgttggtgcc tcccatgccg aacgagctga ccccggcgcg acgcggtgcg ccggagggcg
      181 cgcgccacgg tcggtgctcg cgcacgacct cgaagagatc ggactcgagc ctgagctccg
      241 ggttgggcgc cgtgaagtga accgatggaa ccagctcggc gtgccggagg cacagcgccg
      301 ccttgatcag gccggcgacg ccggccgcgg tgtcgaggtg tccaaggttg ctcttgaccg
      361 agccgagcgc gcagggctct gcgcgcccga cgttcctgaa tacctgctgc agcgcctgca
      421 cctcgatcgg atcgccgagc gcggtccccg agccgtgggc ctcgacgagg ctgatcgtgt
      481 cgggggtgat gccggcgtcg gcgtacgcgg ccgcgatcac ctcggcctgg ccgcgcacgc
      541 tcggcgccgc gaagctgacc ttcgcggcgc cgtcgttgtt gatcgcgctg ccgcggagca
      601 cggcgtagat ccgatcgccg ccggcgagcg cgtccgcgac gcggcggagc acgaccacgc
      661 cggcgccgtt gctgatcacc gtgccgctgg cgccggcgtc gaacgggcgg cagcgcccgt
      721 caggcgagac cacgagcccg ggctgatatg gatagccgtg cttgatcggg aacgtcagcg
      781 tgacgccgcc cgcgatcgcc gcgtcggcct cgccgcgcag gatcgcctgg cgcgccatgt
      841 ggatcgcgac gagcgacgtc gagcaagcgg tctggacgtt gaccgcggga ccggtgagat
      901 cgagcttgtg tgcgacccgc gtcgcgaaga aatcgctgcg cccgccgagg atattgcggt
      961 agtacgaggc aaggtcggca gcggcggccg ggcgctctcc ccactggcca acgccgccga
     1021 acaccgcgac gcggccggcg aaccggtccg gaaccaggcc ggcgtcctcg agcgcctccc
     1081 aggcgcactc gaggaacacg cggtgctgcg gatcgatcag ctcggcgtcg cccggcgagt
     1141 agtcgaagaa cccggcgtcg aaccggtccg cggtcggcac gatgcccttc gcgcccacgt
     1201 agccgggatc ggcgagcgcg gccgtgggga tccccgcggc gcggagctcg tcgcgcgaga
     1261 accgggcgac cgtgtcgagc ccggcgacga ggttgcgcca gtactccgcg agatcgtccg
     1321 cgccgggcac gcggcaggcc atgccgacga tcgcgatcgc gtcatcgggg tcccggcgcg
     1381 cccgccgcgc caccggtgtt gtctcggcgg cgtcgccgag gtaggcggcg agcctggcga
     1441 tcgtcgggta gccaaacagg gcggacagcg ggagctcgcg gccgagccgc gcgacgatcg
     1501 cgcggtgcgc gaccacggcg gtcatcgagg tggcgccgag ctcgaagaag ccgcgatccg
     1561 gcgggacctc cgcgaccccg agcacctcgc gccagacctc ggtcaacagc gcgcgcaacc
     1621 ccgcgccggc ctccccggcg gtggccgccg cgcgcggcgc gggcgttccc atcgggcgct
     1681 ccgcgagcag cgcaccccga tcgatcttgc cgttcggggt gtgcggcagc ccggcgagct
     1741 ggtagagccg cgacggcacc atgtgcggcg ggagctcgcg ggacaggtgc gcctcgagcg
     1801 cggccggatc gacctcggcg ctcgccgcga cccagtacaa gcagagctgc ggatcgtcgg
     1861 ggacgtcggt gccgaccacg gcggcgcact cggcgacgcc agcgaacgcc gtcgcgacgg
     1921 catcgatctc cccgagctcg atgcggtggc cgcgcagctt gatctggtga tcggcgcggc
     1981 cgcaaaactc gaggctgtcg tcgtcgcggt aacgcacgag gtcgccggtc ctgtacatgc
     2041 ggaacccgtc gcctcgccgc ggatcgggga cgaagcgcgc cgcggtgaga tcggggcggc
     2101 ggaggtagcc gcgcgcgacg ccgtcaccgc cgatccacag ctcgccgagc atgccgcgcg
     2161 gaacctcggc gccgtgggcg tcgaggacgt acagggcggt gttggcgatc ggcgcgccga
     2221 gcgagacctc gccgtcgccg acggcccagc acgacgacca gatcgtggtc tccgtcgggc
     2281 cgtacatgtt gatcaccgat ccgcggacca cgcggtcgag ccgcgttgcg agcgtgcggt
     2341 cgagcgcctc gccgccgacg agcaggcggt cgaggtcgcc gagcaggcgg cgcccggcct
     2401 cgtcggcgag cagcatgcgg gcgagcgatg gcgtgcactg gaggtgggtc ggcggcgcgg
     2461 cggtccggcg atccatcgcg gcgagcgcgt cgcgggcgtc gcgagcgacg atcacgtggt
     2521 agccgcgggt cagcgtccac acgagctcga ggccggagat gtcgaacgac gagctcgtga
     2581 ccgcgagcca gcgatcgccc cggccaccgc cgacgacctg gtccatgccc gcgcagaagt
     2641 tcgagaattg atcgtgttcg acgatcacgc ccttgggctt gccggtcgac cccgacgtgt
     2701 agatcacgta tgcggtcgcg tgtggatcca gcgcgacgat gtcgtcgagc gtcgcgccgg
     2761 cgggctcggc gagcaggcag ctcggcacgc cgtcgaagcc ggcggcgtcg gggaggttcg
     2821 tcaccagcag cgcaagcccg ctgtcgtgca cggcatgctc gagcacggcc cgcgggtact
     2881 tcggatcgag cggcacatag gcggcgccga gcatccacac cgcgatcatc gcgaccggca
     2941 ggtcgatcga gcgctcgagc agcaagccga tgagcgtgtc gcgagggatg ccgccgggga
     3001 gatccttcgc tccgccgata gaaccgtcgg gggacccggc cccgcgcgct cgagtcaatc
     3061 gatcgcgcag ggtcgccgcc gcggtgcggg cgcgcgcgac gagctcgcgg taggtcagtg
     3121 tccggcccgc gcactcgacc gcgatcgcgt ccgggtgcgc cgcgccggcg cgcaggatca
     3181 gctcgaccgc cgagacgaac cggcgcgatc gcatcgccgg cagtccggac agcgcgcgga
     3241 gctgtgcggc ctcggcggcg tcgaccgcgc tcagccggtc gatcgcgagc gtcgggtcgg
     3301 cgaccgcgct ggcgagcacg gccgcgtagg cgcgcgcgaa ccgctcggcg ccggtgcgat
     3361 cgagcacgg
//
LOCUS       DASVOR010002354         7525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_493637,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002354 DASVOR010000000
VERSION     DASVOR010002354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7525
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493637"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..850)
                     /locus_tag="VF516_43310"
     CDS             complement(86..850)
                     /locus_tag="VF516_43310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114659.1"
                     /translation="MKRAALALVLAACSSSSNDFPPLPEGAGPGGASGGGGTVGDGGV
                     GDGGSGGAGDAGRVISARVCIVHDLSNPTDCDPKGNASGVKVTLGTRAPITGPDPAGN
                     FTIVAPSGTPVWHLTGSTFVPSVMLYGTDILLPIVPDTLYGDVQGGSGVTILGEGQGS
                     VLARVVSNGAPVANVQVATTFTSTNMIPLYPTRNGLVWITTGPTLASGTVWFPGVDVT
                     SVPGQITFTPQGGVLVPTRVDNVEQGSITFVTQDLP"
     gene            complement(911..1465)
                     /gene="lspA"
                     /locus_tag="VF516_43315"
     CDS             complement(911..1465)
                     /gene="lspA"
                     /locus_tag="VF516_43315"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX8114660.1"
                     /translation="MSPRRGAVAFAVILIAALAADQLSKAWAHTLPVAPAGCALPGEM
                     LAGRCIGVPQPVVDGYWDWELAYNPGVAFSTFASAAGHTGMRVILAAVAVIALVGISL
                     LALRAAPEQRWRRAAYALIAGGALGNLIDRVRDGAVTDFVRWRIHDHRWPIFNVADAV
                     LVVGVVMLLLDGLRARRAPLGRTE"
     gene            complement(1505..2224)
                     /gene="lspA"
                     /locus_tag="VF516_43320"
     CDS             complement(1505..2224)
                     /gene="lspA"
                     /locus_tag="VF516_43320"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEX8114661.1"
                     /translation="MTDPEAASTSSPGSDTGAAGDAGRNKWVVFLIVAVLSLIADQAT
                     KVWARESLPTVRVRSGEAMCIVPDDLVRAPPPAAPGQPVDPRPVCAGRAVPVIDGFWE
                     WRLSMNPGSAFGLFSSREGARIGLSVVGIVAVFGMLWMLRKARPDQKVLHWALALVTG
                     GAIGNLIDRIYFGVVTDFILWRYQTHEWPVFNVADVVLVIGVGLMFIDIQKEGRRDKA
                     LKASRQARAKAAGLVKTFGGK"
     gene            complement(2229..5120)
                     /gene="ileS"
                     /locus_tag="VF516_43325"
     CDS             complement(2229..5120)
                     /gene="ileS"
                     /locus_tag="VF516_43325"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEX8114662.1"
                     /translation="MSGQETKSKYKDTVILPDTKFPMRGDLARREPEILARWDEMKLY
                     ERILAARAGAPLFVLHDGPPYSNGNIHYGHILNKILKDIVVKSRTMAGFRAPYVPGWD
                     THGLPIELAVERDPAIRERRSSLSAAEVRRLCREFALRYVDIQRNEFRRLGVLGDWQH
                     PYLTLDPSYEAAIARALATFARNGYLYRGKKPVVWCPRDKTALAEFEIEYQDHSSPSI
                     YVKMPLVDDGWRGKLDARLEGKRLALVIWTTTPWTLPANLAIVAHPDLTYVAFPNPRD
                     PGESLIVARELAASVARAIGGADLTRAIEIAPAEMAALDGAHYRHPFVAADVSGAAPG
                     DAFRLWFADYVTADTGTGLVHTAPGHGADDFKTGMAHGLPAYAPLDDAGRYIAGVRID
                     GGAELAGLSTEEANPVIVAHLASTGHLLNPPTDRIQHQYAHCWRCKGPIVYRATPQWF
                     IAMDHDQLRERALAEIDRTTWVPAWGHDRIYAMIQNRPDWVLSRQRLWGTPIPTFYCG
                     KCGTPHAAADTMDHVAAIFEREGADAWWTRPVVELVPPGTTCGHCGAGADQLEREKDI
                     VDVWFESGVSWLAMAARDPDHRNIDLYLEGSDQHRGWFHSSLLTGVGVQGKAPYRQVI
                     THGFVLDEHGNPYSKSALARARAEGKQTSYIEPDSVIARSGAELFRLWVGSTEFRTDI
                     AYSQTILDGLSEWYRKLRNTARFLLGNLQDFDPDRHPRAELERRDALTGIDRYLLARL
                     DVVVARARKAYEAYELHVVHRLLVEFVTVDLSALYSDVTKDRLYCDPADSGPRRAAQV
                     VLYECLRALAVLAAPILCFTAEDIWSHMPRRAGDPDSVHLAEFPPAVDDAEAVDVLLQ
                     FDKLLAWRERVNRALEPFRAAGNKSTDARVTLRHPPADPLVNAYGDELADLFIVSEVR
                     LETGDSEDVVVELHPGPRCERCRKHFDALAADPNDVCTRCAAALRAIKG"
     gene            5251..5736
                     /locus_tag="VF516_43330"
     CDS             5251..5736
                     /locus_tag="VF516_43330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEX8114663.1"
                     /translation="MADEPRPRASWDEYFMSIAQVVATRSTCPRKYVGAVIVRNRTIL
                     STGYNGSIRGMPHCSDVGHMMEDGHCVATIHAEANAIIQAARNGVNIDGATSYVTASP
                     CWNCFKQLANAGIVRIVYGEFYRDDRIFEVARQIGIELVHVPLPGVMLPGLPAGPESA
                     H"
     gene            6041..6664
                     /locus_tag="VF516_43335"
     CDS             6041..6664
                     /locus_tag="VF516_43335"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX8114664.1"
                     /translation="MRQCSERGGKTSCRRVAVFQGHNANPSTLRGEPLDRPSGDIWVY
                     AENLGEEFRGNIYKPDGSFDDAALAKLDDMWRDTRSGDVRAVRAELYEHLSRICDHYP
                     DHRVDLVSGFRFHERDSSRHFHASAMDIKIKDVPIRELYDFAQTLDVGVDNEGALGVG
                     IYPTSQFIHVDFRAPGEPSYRWTDWSGHDSGKRKSPGRTQPARKPVS"
     gene            6848..7201
                     /locus_tag="VF516_43340"
     CDS             6848..7201
                     /locus_tag="VF516_43340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114665.1"
                     /translation="MPVTTRIPGRHELERHEHLGARHLPVPDGTHLFERRLEQRMVAG
                     ADQIDVDCLELDLDVDVGLAQFPELLRQLTDHAREAGRLDDEVEVGRGARLGDEADRV
                     SADQRPMHVATRTPR"
BASE COUNT         1109 a         2791 c         2545 g         1080 t
ORIGIN      
        1 cgcgaccgcc gcacccacgg cccgatcagc gccgccgcgc ccgccgcgcc cgccgccgcc
       61 aggaagctcc gccggcgcat cgccgctacg gcaggtcctg cgtgacgaac gtgatcgacc
      121 cctgctcgac gttgtcgacc cgcgtgggga ccaggactcc accctgcggc gtgaaggtga
      181 tctggcccgg tacgctcgtc acgtcgaccc cgggaaacca gaccgtcccg ctggccagcg
      241 tgggtccggt cgtgatccag accaggccgt tgcgggtcgg gtagagcggg atcatgttgg
      301 tgctggtaaa ggtcgtcgcc acctgcacgt tggccaccgg cgcgccgttg ctcaccaccc
      361 gggccagcac cgagccctgg ccctccccca ggatcgtcac gccgctgccg ccctgcacat
      421 cgccgtacag cgtgtcgggc acgatcggca ggaggatgtc ggtgccgtac agcatcaccg
      481 aaggtacgaa ggtggagccg gtcaggtgcc agaccggggt gccggacggc gcgacgatcg
      541 tgaaattgcc ggccgggtcc ggcccggtga tcggcgcccg ggtcccgagc gtcaccttga
      601 cgccggacgc gttccccttg gggtcgcagt ccgtggggtt gctcaggtcg tggacgatgc
      661 acacacgcgc gctgatcacg cgccctgcgt cgccggcgcc gccgctcccc ccgtcgccga
      721 cgcccccgtc cccgacggtg ccaccgccgc cggaggcccc ccccgggccg gccccttccg
      781 ggagcggtgg aaagtcgttg ctgctcgacg agcaggccgc cagcaccagg gcgagggccg
      841 cgcgcttcat gccgggatca tacgcccgcc cggcgcggcg agcgagtttg tcgacggacc
      901 gtgagcgccc tcactccgtg cggcccagcg gtgcacgccg ggcgcgcagc ccgtcgagca
      961 gcagcatcac cacgccgacc accagcaccg cgtccgccac gttgaagatc ggccagcgat
     1021 ggtcgtggat ccgccagcgc acgaagtcgg tcaccgcgcc gtcgcgcacg cggtcgatca
     1081 ggttgcccag cgcgccgccg gcgatcagcg cgtacgccgc gcgccgccag cgctgctcgg
     1141 gcgccgcgcg cagcgcgagc aggctgatcc cgaccagcgc gatcaccgcg accgccgcca
     1201 ggatcacgcg catgccggtg tggcccgcgg ccgacgcgaa cgtcgagaac gcgacgcccg
     1261 ggttgtacgc cagctcccag tcccagtagc cgtcgaccac cggctggggc acgccgatgc
     1321 agcgccccgc gagcatctcg ccgggcaagg cgcagcccgc cggcgcgacc ggcagcgtgt
     1381 gcgcccacgc cttgctcagc tgatcggccg cgagcgcagc gatcaagatc acggcgaacg
     1441 cgacggcgcc acggcggggg ctcatgacgg accaaccgcc gccggagccg cggctatttc
     1501 cgcgtcactt gccgccgaac gtcttgacca gccccgcggc ctttgcccgg gcttgccggc
     1561 tcgccttgag cgccttgtcg cggcggcctt ccttctggat gtcgatgaac atcaggccga
     1621 ccccgatgac cagcacgaca tcggcgacgt tgaacaccgg ccactcgtgg gtctggtagc
     1681 gccacaggat gaagtcggtg acgaccccga agtagatccg gtcgatcagg ttgccgatcg
     1741 ccccgccggt gaccagcgcg agcgcccagt gcaggacctt ctggtccggc cgcgccttgc
     1801 gcagcatcca cagcatgccg aacaccgcga cgatcccgac gaccgacagc ccgatccgcg
     1861 cgccctcccg gctggagaac agcccgaacg ccgagccggg gttcatcgac agccgccact
     1921 cccagaaccc gtcgatcacc ggcaccgcgc gccccgcgca gaccggacgc ggatcgacag
     1981 gctgcccggg ggccgcgggt ggcggcgccc ggaccagatc gtcgggcacg atgcacatcg
     2041 cttcgccgct ccggacccgg acggtcggca gcgactcgcg cgcccagacc ttggtcgcct
     2101 gatccgcgat cagcgacagc accgcgacga tcaggaacac cacccacttg ttccggccgg
     2161 cgtcgccggc ggccccggta tcggaaccag gggacgaggt ggacgcggcc tcgggatcgg
     2221 tcatcgtcct agccctttat cgcgcgcagc gccgcggcgc aacgcgtaca tacgtcgttc
     2281 gggtcggcgg cgagcgcgtc gaagtgcttg cggcagcgct cgcagcgcgg gccggggtgg
     2341 agctcgacca cgacgtcctc gctgtccccg gtctccagcc ggacctccga cacgatgaac
     2401 agatcggcga gctcgtcgcc gtaggcgttg accagcggat cggccggcgg gtggcgcagc
     2461 gtgacccgcg cgtcggtcga cttgttgccg gcggcgcgga acggctcgag cgcgcggttg
     2521 acccgctcgc gccacgccag cagcttgtcg aactgcagca gcacgtcgac cgcctcggcg
     2581 tcgtcgaccg ccggcgggaa ctccgcgagg tgcacgctgt ccggatcgcc ggcgcggcgc
     2641 ggcatgtggc tccagatgtc ctcggcggtg aagcacagga tcggcgccgc cagcaccgcc
     2701 agcgcgcgca ggcactcgta cagcaccacc tgggcggcgc ggcgcggccc cgagtccgcc
     2761 ggatcgcagt acagccggtc cttggtgacg tcggagtaca gcgccgacag gtcgaccgtg
     2821 acgaactcga ccagcaggcg gtgcacgacg tggagctcgt aggcctcgta cgccttgcgc
     2881 gcccgcgcga ccaccacgtc gagccgggcc aagaggtagc gatcgatccc ggtcagcgcg
     2941 tcgcggcgct cgagctcggc gcgagggtgc cggtcggggt cgaagtcctg gaggttgccg
     3001 agcaggaacc gcgcggtgtt gcgcagcttg cggtaccact ccgacagccc gtcgaggatg
     3061 gtctgcgagt acgcgatgtc ggtgcggaac tcggtcgagc cgacccacag ccggaacagc
     3121 tcggccccgc tcctggcgat cacgctgtcg ggctcgatgt agctggtctg cttgccctcg
     3181 gcgcgggccc tcgcgagcgc cgacttcgag tacgggttgc cgtgctcgtc cagcacgaac
     3241 ccgtgcgtga tcacctggcg gtacggcgcc ttgccctgga cgccgacccc ggtgagcagc
     3301 gacgagtgga accagccgcg gtgctgatcc gagccctcga ggtagaggtc gatgttgcgg
     3361 tggtcggggt cgcgcgccgc catcgccagc cacgacacgc cggactcgaa ccacacgtcg
     3421 acgatgtcct tctcgcgctc gagctggtcg gccccggcgc cgcagtggcc gcacgtcgtg
     3481 cccggcggca ccagctcgac caccggccgc gtccaccacg cgtcggcgcc ctcgcgctcg
     3541 aagatcgcgg cgacgtggtc catcgtgtcg gccgcggcgt gcggcgtgcc gcacttcccg
     3601 cagtagaacg tcgggatcgg cgtgccccac agccgctggc gcgacagcac ccagtccggc
     3661 cggttctgga tcatcgcgta gatccggtcg tggccccacg ccgggaccca cgtcgtgcga
     3721 tcgatctcgg ccagcgcgcg ctcgcgcagc tgatcgtggt ccatcgcgat gaaccactgc
     3781 ggcgtcgcgc gatacacgat cggccccttg caccgccagc agtgcgcgta ctggtgctgg
     3841 atacgatcgg tcggcgggtt gagcaggtgc ccggtgctcg ccaggtgcgc gacgatcacc
     3901 gggttggcct cctcggtcga cagcccggcg agctcggcgc cgccgtcgat ccgcaccccg
     3961 gcgatgtagc ggccggcgtc gtcgagcggc gcgtacgccg gcagcccgtg cgccatgccg
     4021 gtcttgaagt cgtcggcgcc gtggccgggc gcggtgtgga ccagcccggt gccggtgtcc
     4081 gcggtcacgt agtcggcgaa ccacagccgg aacgcgtcgc ccggcgccgc gccgctgacg
     4141 tcggccgcga cgaacgggtg ccggtagtgt gcgccgtcga gcgccgccat ctcggccggc
     4201 gcgatctcga tcgcccgggt caggtcggcg ccgccgatcg cgcgggccac cgacgccgcc
     4261 agctcgcgcg ccacgatcag ggactctccg ggatcgcgcg ggttggggaa cgcgacatag
     4321 gtcagatccg gatgcgccac gatcgccagg ttggccggca gcgtccacgg cgtcgtcgtc
     4381 cagatcacca gcgcgagccg cttgccttcg agccgcgcgt cgagcttgcc gcgccagccg
     4441 tcgtcgacca gcggcatctt cacgtagatg ctcggcgagc tgtggtcctg gtactcgatc
     4501 tcgaactcgg cgagcgcggt cttgtcgcgc gggcaccaca ccaccggctt cttgccgcgg
     4561 tacaggtagc cgttgcgcgc gaacgtcgcc agcgcgcgcg cgatcgccgc ctcgtagctc
     4621 ggatcgagcg tcaggtacgg gtgctgccag tcgcccagca cgccgagccg gcggaactcg
     4681 ttgcgctgga tgtcgacgta gcgcagcgcg aactcgcggc acagccggcg gacctcggcc
     4741 gcggacagcg agctccgccg ctcgcggatc gccggatcgc gctcgaccgc gagctcgatc
     4801 ggcaggccgt gggtatccca gcccggcacg tagggcgcgc ggaaccccgc catcgtccgc
     4861 gacttgacca cgatgtcctt gaggatcttg ttgaggatgt ggccgtagtg gatgttgccg
     4921 ttggagtacg gcggcccgtc gtgcagcacg aacagcggcg cgccggcgcg tgcggcgagg
     4981 atccgctcgt acagcttcat ctcgtcccac cgcgcgagga tctccggctc ccgccgggcc
     5041 agatcgccgc gcatcgggaa cttggtgtcc ggcaggatca ccgtgtcctt gtatttcgac
     5101 ttcgtctcct gacccgacat caggcggcga ccatagccga tcccaccgcc gccgtccgca
     5161 cgccccggcc ccggtcaccg tcgccccggc cgtcgccccg gccctcgccc cggcccggct
     5221 caccgtcgca ccgggctgct aggatgccgc atggccgacg aaccccgccc ccgcgcctcg
     5281 tgggacgaat acttcatgag catcgcgcag gtcgtcgcga cccgctcgac ctgcccgcgc
     5341 aagtacgtcg gcgcggtgat cgtccgcaac cggacgatcc tgtcgaccgg ctacaacggc
     5401 tcgatccgcg gcatgccgca ctgctcggac gtcggccaca tgatggagga cggccactgc
     5461 gtcgccacga tccacgccga ggccaacgcg atcatccagg ccgcgcgcaa cggcgtgaac
     5521 atcgacgggg cgaccagcta cgtcaccgcg agcccgtgct ggaactgctt caagcagctc
     5581 gccaacgccg gcatcgtgcg catcgtctac ggcgagttct atcgcgacga ccggatcttc
     5641 gaggtcgcgc gccagatcgg catcgagctc gtccacgtcc cgctgccggg ggtcatgttg
     5701 cccgggctcc ccgcggggcc cgagagcgcg cattgatcac atctgggaca tcggcgacca
     5761 ctgccccact gaacatctgg cgcaccgacc tgaacacctg tctgcaacgc tccgttcgaa
     5821 cgcaaatcaa gggccagcga gggatttccc gtcggtattt ggttgagcca gtgcatcgct
     5881 cagcctagcc tcgggtccta tgcgccggtt tgccctgctc atcgtcgcag ccgctgcgtc
     5941 tgttgtcgcc tccggtgctt ctgggaccgc gtccgcggat tcgtcgatcc atcccggcgc
     6001 gaaggccgcc gcccggcgga gcatcaacga caagggcacg atgcggcaat gcagcgagcg
     6061 cggcggcaag accagctgcc ggcgagtcgc ggtgttccag ggccacaacg ccaacccatc
     6121 gaccctgcgc ggcgagccgc tcgaccggcc gtcgggcgac atctgggtct atgccgagaa
     6181 cctcggcgag gagttccgcg gcaacatcta caagcccgac ggcagcttcg acgacgccgc
     6241 gctcgccaag ctcgacgaca tgtggcgcga caccaggagc ggcgacgtcc gcgccgtgcg
     6301 cgccgagctc tacgagcacc tgtcccggat ctgcgatcac taccccgacc accgtgtcga
     6361 cctggtctcc ggcttccggt ttcacgagcg cgactccagc cgtcacttcc acgcctcggc
     6421 gatggacatc aagatcaagg acgtcccgat ccgcgagctc tacgacttcg cccagaccct
     6481 cgacgtcggg gtcgacaacg agggcgcgct cggcgtcggc atctacccga ccagccagtt
     6541 catccacgtc gacttccggg cgcccggcga gccgagctac cgctggaccg actggtcggg
     6601 gcacgacagc ggcaagcgca agtcgccggg ccgcacccag ccggcccgca agccggtcag
     6661 ctgagcgcgg cgcggcgcct cggatcgcga tcggctcgcc gcggtcgatc ttgtcgagca
     6721 attcgccgaa ctcgcgctcg gccccgggat agaccagccg ggacagctcg atcatcgcgg
     6781 cgcggtcgag gtcccgctgg ccgacctcgt agaggcagca gagatcggcc atatcttgcg
     6841 cgcgtcgatg ccggtcacga cgcggattcc tggccgccat gaacttgagc gccacgagca
     6901 cctcggtgcg cggcaccttc cagtccccga cggtacgcac ctgttcgagc gccgcctgga
     6961 acagcgcatg gttgctggag cggatcagat cgacgtcgat tgtcttgagc tggatctcga
     7021 cgtcgatgtc ggtctcgccc aattccccga gctccttcgc cagctgacgg accacgctcg
     7081 cgaggccggc cgtctcgacg acgaagtcga agtcgggcgt ggcgcgcggc tcggtgacga
     7141 ggccgatcgc gtgtctgccg accagcgccc aatgcacgtg gcaactcgaa cgcctcgatg
     7201 acccggcaga tgtccgcctc gctcatcgtc ctgcctgctc gctccgcgcc gtcccgccgg
     7261 ccgaacagcg cccggtgggt cgcgaggatc cgcatcgagc gccggatcgg ctcgggaatt
     7321 ccggcggggg acatctggct agcatatcat cagcgatagc gtctgcccgc cacggcgaga
     7381 tccgcgacga cgtcgtcgat ttcgagagcg tcgtttccct ccttgtcgcg atgctgcgac
     7441 ctcactggcc gggcgatgga tcctgctccg cagcgcggtg gttccctgtt cgaccgcatc
     7501 cccgtgcagg ggaacgcgcg gcgag
//
LOCUS       DASVOR010002355         4905 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_171874,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002355 DASVOR010000000
VERSION     DASVOR010002355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_171874"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2538
                     /locus_tag="VF516_43345"
     CDS             <1..2538
                     /locus_tag="VF516_43345"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX8114666.1"
                     /translation="TRPPRTATASLRPSGLAPAGSTPALAVTPFAFGASAYHLQIARD
                     AAFTSVVVDTQVSEPTFNVPFDLDIATQYFWRVSATNACGASAFSPVASFIVGACFES
                     WSQASAFPSGGVSQASVIASPADGKLYVISGSGPTDQLWAFDPQAGSWARKADVPAPG
                     MGASFGSAVRLGGTIYVFGGQNFFTTHRVLWRYDIASDTWSRGADLPLANFGAAVAAI
                     NGKIYLAYGSGFVTQTWQYDPATNSYTRKADAPFVPQTFRLHGVAVNGELHAFAGGFN
                     GTAHVIYNPATDTWRTGAAMPFGATDPVVDVLGNKVFVVGGTPVAHTQVYDPATDSWS
                     QAASISGATGGVDNTEGAVLGSVLHLIGGVSGFGNVTTHWQFHACSLGALASATIIPF
                     VVDGDGKVANIGNENTSILIDNSVSGAQMSVSCFLYGTTGEVLGSDTFQVGANELATV
                     PNVVRALTHTSTVQNQIGSVAMFGTEVFHGMASISNNASSDPAFEDGRPIAGTTSGFL
                     SSIGAQGYLTQTVFTNTSSSTSSLQIVAYPPGGGDTPAAATLVFLPPHGLVSYPDVVK
                     KLGLAAGFVGQLSWTSSQPTTVAARELTRKKDFSGFDPAHAPGEATSSVVVPYVEDTD
                     AFSTALEISNPGSFTANVTVNFVEISDTTGATSGVQHTRDLPVAVNSASPIADIVRWA
                     QRDTSTAHSGKHGFLVVTTPQAVTAQARIVDNANLDPGVPDSGGVASAFSSLLVRVES
                     LPFSQIGAAGSSPDSASRFALSNPSSAPATVQLVAFNATGSAASSHPLVVTLAPHGQF
                     FTANLAAAMGLPSVFLGWVAIQSDVPVGVYNHRRTGTVGSVVPLHRH"
     gene            2712..4574
                     /locus_tag="VF516_43350"
     CDS             2712..4574
                     /locus_tag="VF516_43350"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8114667.1"
                     /translation="MPILVLALASGCAHAPAYSPGDERLEAIEFEGNHQLGDKTLITG
                     LALHRTQQRGGAPDPYLIAIDADRIRGEYLRKGFLDVDVRSRVDRRGDAVTVTYTVEE
                     GPRAATRVVITGLPPDVPAAAVRARLPLRDGQPFDYEPYDLAKPLLLGVVQDAGYARA
                     RLDARVIADRADHVAIVDLAYTPGPKCRFGGVEIAGATGELATAVRARLAFQPGQVYS
                     TQAITQTQRNLYGFARFSTVQVQPDQGTGEVVNVKVSVAEGARHQVQLGGGFGRDTVG
                     YSVHGRAGYSVAGWPFPLDTSSITLRPEYVYNTYVGSEPRIRARARLERADLLWTYTK
                     ANIDAGYDYLTWEAYTSYGPLVRIGFETPVTTPRIRLWLGWRLEQNAFRDITTIIDPP
                     IAAVVRQGNADKINPAAVALKHTLGLDHSERLGGYGELRITEGTPLAGGAFNYVEAIG
                     DVRGFAPLLLDGAVLAARARYGGIWGDIPVTERLFSGGAVSQRGFGERRLAPFVHGTT
                     FNPDGTVSGSAYIPYGGGGLFETSLEARVPITKVRTMPLGIVAFLDGGDVTETPGGLD
                     FGNLHWAVGGGLRVITPVGPVRLDVGYRLNRTSGPNNPDPGQRFAYHLTIGEAF"
     gene            4574..>4905
                     /locus_tag="VF516_43355"
     CDS             4574..>4905
                     /locus_tag="VF516_43355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114668.1"
                     /translation="MSAQRDPRRGWRWLRRGALGVIGFAMLAVLGAMIAMQTRWGREL
                     VRAQVEQRLAATFTGGATLGGLEGNPFSKLTLHDVVINGADRRPAITVKQLTVTVGLL
                     PLLSHQARV"
BASE COUNT          688 a         1818 c         1707 g          692 t
ORIGIN      
        1 acccgcccgc cccgcacggc taccgcgagc ctccggccgt ccggcctggc gccggcgggc
       61 tcgacgccgg cgctcgccgt gaccccgttc gcgttcggcg cctcggcgta ccacctccag
      121 atcgcgcgcg acgcggcgtt caccagcgtg gtcgtcgaca cccaggtcag cgagcccacg
      181 ttcaacgtgc cgttcgacct cgacatcgcg acccagtact tctggcgggt cagcgcgacc
      241 aacgcgtgcg gcgccagcgc gttctcgccc gtcgcctcgt tcatcgtcgg cgcgtgcttc
      301 gagagctggt cccaggcgtc ggcgttcccg agcggcggtg tctcgcaggc gtccgtgatc
      361 gcctcgccgg ccgacggcaa gctctacgtc atcagcggca gcgggccgac cgaccagctg
      421 tgggcattcg atccgcaggc cgggagctgg gcgcgcaagg ccgacgtccc ggcgccgggc
      481 atgggcgcga gcttcggttc cgcggtccgg ctcggaggca cgatctacgt gttcggcggg
      541 cagaacttct tcacgacgca ccgggtgctg tggcggtacg acatcgcgag cgacacctgg
      601 agccgcgggg ccgatctgcc gctcgctaac ttcggcgcgg cggtcgcggc gatcaacggc
      661 aagatctacc tcgcctacgg ctcgggcttc gtcacccaga cctggcagta cgatccggcc
      721 accaacagct acacccgcaa ggccgacgca ccgttcgtcc cgcagacctt ccgcctgcac
      781 ggcgtcgccg tcaacggcga gctccacgcg ttcgccggcg gcttcaacgg cacggcacac
      841 gtgatctaca acccggcgac cgacacgtgg cggaccggcg cggcgatgcc gttcggtgcg
      901 accgatccgg tggtcgacgt gctcggcaac aaggtgttcg tcgtcggcgg caccccggtg
      961 gcccacaccc aggtctacga cccggcgacc gacagctggt cccaggcggc atcgatcagc
     1021 ggtgccaccg gcggcgtgga caacaccgag ggcgcggtcc tcgggtcggt gctccacctg
     1081 atcggcggcg tcagcggctt cggcaacgtg accacgcact ggcagttcca cgcctgcagc
     1141 ctcggcgcgc tggcgtcggc gaccatcatc ccgttcgtgg tcgatggcga cggcaaggtc
     1201 gcgaacatcg gcaacgagaa cacgtcgatc ctgatcgaca actcggtcag cggcgcgcag
     1261 atgtcggtca gctgcttcct ctacgggacc accggcgagg tgctcgggag cgacacgttc
     1321 caggtcgggg ccaacgagct ggccaccgtc cccaacgtgg tccgcgcgct gacccacacg
     1381 tcgaccgtgc agaaccagat cggcagcgtg gcgatgttcg gcaccgaggt gttccacggc
     1441 atggcctcga tctccaacaa cgcgagctcc gatccggcgt tcgaggacgg ccggccgatc
     1501 gccggcacca ccagcgggtt cctgtcgagc atcggggccc agggctacct gacccagacc
     1561 gtgttcacca acacctccag cagcacgtcg tcgctccaga tcgtcgcgta tccgccgggc
     1621 ggcggcgaca cgccggcggc ggcgaccctg gtgttcctcc cgccgcacgg cctggtgagc
     1681 tatccggacg tcgtcaagaa gctggggctg gccgcgggct tcgtcgggca gctgagctgg
     1741 acgtcgagcc agcccaccac ggtcgcggcg cgcgagctga cgcgcaagaa ggacttcagc
     1801 ggcttcgatc cggcgcacgc ccccggcgag gccacctcga gcgtggtcgt gccgtacgtc
     1861 gaggacaccg acgcgttcag caccgcgctc gagatcagca acccggggtc cttcaccgcc
     1921 aacgtgacgg tgaacttcgt cgagatcagc gacaccacgg gcgcgacctc gggcgtgcag
     1981 cacacgcgcg acctcccggt ggcggtgaac agcgcgtcgc cgatcgccga catcgtgcgc
     2041 tgggcgcagc gcgacacctc gaccgcgcac tcgggcaagc acggcttcct ggtggtcacc
     2101 accccgcagg cggtgaccgc gcaggcccgg atcgtcgaca acgccaacct cgatcccggg
     2161 gtaccggaca gcggcggggt cgcgagcgcg ttctcgtcct tgctggtccg cgtcgagtcg
     2221 ctgccgttct cgcagatcgg cgccgccggc tcgtcgcccg acagcgcgtc gcgattcgcg
     2281 ctgtcgaacc cgagctcggc gccggccacc gtgcagctcg tcgcgttcaa cgcgaccggc
     2341 tccgcggcga gcagccaccc gctggtggtg accctggcgc cgcacggcca gttcttcacc
     2401 gccaacctgg ccgccgccat ggggctgccg tcggtgttcc tcggctgggt cgccatccag
     2461 tccgatgtcc cggtgggggt ctacaaccat cgccggaccg gcacggtcgg cagcgtcgtc
     2521 ccgttgcacc ggcactgagc gctgctcctc ggagttgctg caccggggcg tcggtggcgc
     2581 cgccggcgct cgccgcgcgc ggaagcaaca cccggatcac ggcacgtggt gaccgccctg
     2641 cacgccaccg ctgcggggcg gcgtgccttc gcgcaggtgt cgttcttgcg gggggcgatg
     2701 gcatgcggcc cgtgccgatc ctcgtcctcg cgctcgcaag cggctgcgcc cacgccccgg
     2761 cgtactcgcc cggcgacgag cgcctcgagg cgatcgagtt cgagggcaac catcagctcg
     2821 gcgacaagac gctgatcacc gggctcgcgc tgcaccggac gcagcagcgc ggcggcgcgc
     2881 ccgatccata tctgatcgcg atcgacgccg accggatccg cggcgagtac ctgcgcaagg
     2941 gcttcctcga tgtcgacgtc cgatcgcggg tcgatcgccg gggcgacgcg gtgaccgtga
     3001 cgtacaccgt cgaggagggg ccgcgcgcgg cgacccgcgt cgtgatcacc gggctgccgc
     3061 ccgacgtccc ggccgcggcg gtgcgcgcca ggctgccgct gcgcgacggc cagccgttcg
     3121 attacgagcc ctacgacctc gccaagccgc tgctgctcgg cgtggtccag gacgccggct
     3181 acgcgcgcgc tcggctcgat gcccgcgtga tcgccgaccg ggccgaccac gtcgcgatcg
     3241 tcgacctcgc ctacaccccc ggcccgaagt gccggttcgg cggcgtcgag atcgccggcg
     3301 cgaccggcga gctggcgacc gcggtgcgcg cgcggctcgc gttccagccc ggccaggtgt
     3361 actcgaccca ggcgatcacc cagacccagc gcaacctgta cggcttcgcc cggttctcga
     3421 ccgtgcaggt ccagcccgat cagggcaccg gcgaggtggt caacgtcaag gtctcggtcg
     3481 ccgagggcgc gcgccaccag gtccagctcg gcggcgggtt cggccgggac acggtcggct
     3541 actcggtgca cggccgcgcc gggtactcgg tcgcaggctg gccgttcccg ctcgacacgt
     3601 cgtcgatcac gctccggccg gagtacgtgt acaacaccta cgtcggcagc gagccgcgga
     3661 tccgcgcgcg cgcccgcctg gagcgcgccg acctgctctg gacctacacc aaggccaaca
     3721 tcgacgccgg ctacgactac ctgacctggg aggcgtacac cagctacggg ccgctcgtgc
     3781 ggatcgggtt cgagaccccg gtgacgaccc cgcggatccg gctgtggctc ggctggcggc
     3841 tcgagcagaa cgcgttccgc gacatcacga cgatcatcga cccgccgatc gcggcggtgg
     3901 tgaggcaggg caatgcggac aagatcaacc ccgccgcggt cgcgctgaag cacacgctcg
     3961 gcctcgatca cagcgagcgg ctcgggggct acggcgagct gcgcatcacc gagggcacgc
     4021 ccctggcggg cggtgcgttc aactacgtcg aggcgatcgg cgacgtgcgc ggcttcgcgc
     4081 cgctgctgct cgacggcgcc gtgctcgcgg cgcgcgcccg ctacggcggg atctggggcg
     4141 acatcccggt gaccgagcgg ctgttctcgg gcggcgcggt cagccagcgc gggttcggcg
     4201 agcgccggct cgcaccgttc gtccacggca ccacgttcaa cccggacggc acggtcagcg
     4261 gctcggcgta catcccgtac ggcggcgggg gtctgttcga gaccagcctg gaggcccggg
     4321 tgccgatcac caaggtccgg accatgccgc tcgggatcgt cgcgttcctc gacggcggcg
     4381 atgtcaccga gaccccgggc ggcctcgact tcggaaacct gcactgggcg gtgggcggcg
     4441 ggctgcgggt gatcacgccg gtcggcccgg tccggctcga cgtcggctac cggctgaacc
     4501 gcacgtcggg ccccaacaat ccggatcccg gacagcgctt cgcatatcac ctcacgatcg
     4561 gggaggcgtt ctgatgtccg cgcagcgcga tccacggcgc ggctggcggt ggctccggcg
     4621 cggcgcgctc ggcgtcatcg ggttcgcgat gctcgccgtg ctcggggcga tgatcgcgat
     4681 gcagacccgg tggggccgcg agctggtccg cgcccaggtc gagcagcggc tcgcagcgac
     4741 gttcaccggc ggggcgacgc tcggcgggct cgagggcaac ccgttcagca agctcacgct
     4801 gcacgacgtc gtgatcaacg gggccgatcg ccggccggcg atcaccgtca agcagctgac
     4861 cgtcacggtc ggcctcttgc cgctgctgtc gcaccaggcg cgcgt
//
LOCUS       DASVOR010002356         3258 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_463973,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002356 DASVOR010000000
VERSION     DASVOR010002356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463973"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..414)
                     /locus_tag="VF516_43360"
     CDS             complement(<1..414)
                     /locus_tag="VF516_43360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114669.1"
                     /translation="MMYVQAAAGGVLLISVDEPAGRSLVSALETATIQTEWLRSLDEL
                     HSRAARADASTPALVFLDLELPDAAAEQLVPLVRTCFPTATTIALATELSGESAARLL
                     SQGVPSLTKPVSPLALTGLALRLSLGAGAAWPQRLA"
     gene            complement(766..1371)
                     /locus_tag="VF516_43365"
     CDS             complement(766..1371)
                     /locus_tag="VF516_43365"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEX8114670.1"
                     /translation="MLTYKIVQVGDPVLRRRARELSRDEIATPAIQDLIIRMRDTMRD
                     APGVGLAAPQIGESIQLAVIEDPPQAHAGMTPAQLAERERSAVAFHVLINPRLTVEGD
                     EQVAAYEGCLSLAGFSMIVPRWRRVRVEALDEHGDPVVKVASGWYARILQHEIDHLRG
                     VVCCDRMEPRSLTTQDNHLRYWRTMPVDAVRAVLGSTPADT"
     gene            complement(1414..2256)
                     /locus_tag="VF516_43370"
     CDS             complement(1414..2256)
                     /locus_tag="VF516_43370"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEX8114671.1"
                     /translation="MDPAGYQVALDVFEGPLDLLLHLVKKHELSILDIPIAFVTEKYL
                     EYLDAMAALDIDVAGEYLLMAATLCHIKSRELLPTPEPLEDEEGEAGEGGEVEVDPRA
                     DLIRRLLEYQKYKEAAQQLGQQPVVGRNVWSRGAPVEDAVSDGVDPDAIAPLAPFPVH
                     KLIEAFDRVMQQAKLKVSHDVMIDRLSVSQKISELTDRLEAEGRFTFTSMFSFLRDGL
                     PRAIAEVRHEAVVTFLALLEMAKLRLIALSQPEGDDEIVIERAGEDLRSRMEHGVARS
                     DDYR"
     gene            complement(2274..2912)
                     /locus_tag="VF516_43375"
     CDS             complement(2274..2912)
                     /locus_tag="VF516_43375"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEX8114672.1"
                     /translation="MLYVLILLISVALHEFGHAFMADRLGDDTPRRQGRVTLNPLAHA
                     DLIGTIALPLLGGFLGGGFGWGKPVQWQPHRVSRRISMSTAKILVSVAGPAMNLVLGT
                     VLALVHTILVSQHVIDGAGPFSQIFWAASTTNYVLFFFNLLPVPPLDGGHVAQSFLPY
                     RYRNQYAEVARYAPFALLAVMFIPQLRLVFLWPANQVTDLVYHGFGHLFGPA"
     gene            3007..>3258
                     /locus_tag="VF516_43380"
     CDS             3007..>3258
                     /locus_tag="VF516_43380"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX8114673.1"
                     /translation="MRRARGRPDDRARARRLSLDEGVDLFLDHVKVERGLARHTLDAY
                     GRDLARFTAFLAARGRADADDITPLDVTDFLIQLAADGLA"
BASE COUNT          529 a         1156 c         1085 g          488 t
ORIGIN      
        1 cgcgaggcgc tgcggccacg ccgcgccggc gccgagcgac agccgcagtg cgaggccggt
       61 cagcgcgagc gggctgaccg gcttggtcag cgacgggaca ccctggctca gcagccgcgc
      121 cgccgactcg ccgctgagct cggtggcgag cgcgatcgtg gtcgcggtcg gaaagcaggt
      181 ccggaccagc ggcaccagct gctcggcggc ggcgtcgggc aactcgagat cgaggaacac
      241 cagcgcgggc gtggacgcgt cggcgcgcgc cgcgcggctg tgaagctcgt cgagcgagcg
      301 caaccattcg gtctggatcg tcgcggtctc cagcgcgctg accagcgagc gtcccgcagg
      361 ctcgtcgacg gagatcaaca gcacgccgcc ggccgccgct tggacataca tcatgactgc
      421 ctcattgata accatcacag gatacttaat ttatttttgc caatcgataa caacttcaat
      481 tgttagaata cttacgtgtg ccgccgcgca tcttgacttt tcctcggtgt cggttttttg
      541 actcgcgtgc gcgatcgaga atcctcgcgg cgacagcaac atcgccggtg acccgctcgc
      601 ggcggcctca gagaatcgat cgccgatagc gaattttcac gggctcgcgc gccccgggat
      661 gtcggacgag gtggtcggcg cgctgcgcga tgagatggcg accggtcggt ccggcgatgc
      721 gccatgagca acgctcatgc aacaacgtga acttgttatc gattattatg tatccgcggg
      781 cgtcgatccc agcacagcgc ggaccgcgtc gaccggcatg gtgcgccagt accgcaggtg
      841 gttgtcctgc gtcgtgagcg agcgcggctc catccggtcg cagcacacca cgccgcgcag
      901 gtggtcgatc tcgtgctgca ggatccgcgc gtaccagccc gacgcgacct tgaccaccgg
      961 atcgccatgc tcgtcgagcg cctcgacgcg gacccgccgc cagcgcggca cgatcatcga
     1021 gaacccggcc aggctcaggc agccctcgta ggccgcgacc tgctcgtcgc cctcgacggt
     1081 cagccgcggg ttgatcagga cgtggaacgc caccgcggag cgctcgcgct cggcgagctg
     1141 cgccggcgtc atcccggcgt gcgcctgcgg cggatcctcg atcaccgcga gctggatcga
     1201 ctcgccgatc tgcggcgccg cgagcccgac ccccggcgcg tcgcgcatcg tgtcccgcat
     1261 ccggatgatc aggtcctgga tcgcgggcgt cgcgatctcg tcgcggctca gctcgcgcgc
     1321 acggcgccgc agcaccggat cccccacttg cacgatcttg tacgtcagca cgcccgaacc
     1381 ctaccctccc cgctcgccgg gacgccagcc ggctcagcgg tagtcgtcgg accgcgccac
     1441 gccgtgctcc atccggctgc gcaggtcctc gccggcccgc tcgatgacga tctcgtcgtc
     1501 gccctccggc tgggacagcg cgatcagccg cagcttggcc atctcgagca gcgccaggaa
     1561 cgtcaccacc gcctcgtggc gaacctcggc gatcgcccgc ggcaggccgt cgcgcaagaa
     1621 gctgaacatc gaggtgaacg tgaaccggcc ctcggcttcc agccggtcgg tcagctcgga
     1681 gatcttctgg ctgacgctga gccggtcgat catcacgtcg tgcgagacct tgagcttggc
     1741 ctgctgcatg acgcggtcga acgcctcgat cagcttgtgc accgggaacg gcgcgagcgg
     1801 cgcgatcgca tcgggatcga cgccgtcgct gaccgcgtcc tcgaccggcg cgccgcgcga
     1861 ccacacgttg cggccgacca ccggctgctg accgagctgc tgcgccgctt ccttgtactt
     1921 ctggtactcg agcagccggc ggatcaggtc ggcgcgcgga tcgacctcga cctcgccacc
     1981 ctcgccggcc tcgccttcct cgtcctcgag cggctcgggc gtcggcaaca gctcgcgcga
     2041 cttgatgtgg cacaaggtcg ccgccatcag caggtactcg ccggcgacgt cgatgtcgag
     2101 cgccgccatc gcgtcgaggt actcgaggta cttctcggtg acgaacgcga tcgggatgtc
     2161 gaggatcgac agctcgtgct tcttgaccag gtggagcaag aggtcgagcg ggccctcgaa
     2221 cacgtcgagg gcgacctggt agccggcggg atccatgcgc gcctacgcca cgcctacgcc
     2281 ggaccgaaca ggtggccgaa cccgtggtag acgagatcag tgacctggtt ggcgggccac
     2341 aggaacacca gccggagctg cgggatgaac atcaccgcga gcagggcgaa cggtgcgtag
     2401 cgcgccacct ccgcgtactg gttgcggtag cggtagggca gaaagctctg cgccacgtgg
     2461 ccgccgtcga gcggaggcac cggcagcagg ttgaagaaga acagcacgta gttggtggtc
     2521 gacgcggccc agaagatctg gctgaaaggg cccgccccgt cgatcacgtg ctgcgacacc
     2581 aggatggtat ggaccagcgc gagcaccgtc ccgagcacga ggttcatcgc cgggcccgcg
     2641 accgagacga ggatcttcgc ggtcgacatc gagatccggc gcgacacgcg gtggggctgc
     2701 cactgcaccg gcttgcccca gccgaaacca ccgcccagga acccgcccag cagcggcagc
     2761 gcgatcgtgc cgatcagatc ggcgtgcgcc agcgggttca gcgtgacccg gccctggcgc
     2821 cgcggcgtgt cgtcgccgag ccggtccgcc atgaaggcgt ggccgaactc gtgcaaagcc
     2881 acggagatca gcaggatcag gacgtagagc aacgcccagc gaacggtttc ggatgagaca
     2941 tccatgccag gtctcggtcg ctggactatg gacggtcgcg gcggtgactg caaccccttc
     3001 agaaacttgc gccgcgcgcg cggccgcccc gacgatcgcg cccgggcgcg ccggctcagc
     3061 ctcgacgagg gcgtcgacct gttcctcgac cacgtcaagg tcgagcgcgg gctcgcgcgc
     3121 cacacgctcg acgcctacgg ccgcgacctc gcgcggttca ccgcgttcct cgccgcgcgc
     3181 ggccgcgccg acgccgacga catcactccg ctcgacgtca ccgacttcct gatccagctc
     3241 gccgccgacg gcctggcc
//
LOCUS       DASVOR010002357         5218 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_434262,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002357 DASVOR010000000
VERSION     DASVOR010002357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5218
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434262"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..762
                     /locus_tag="VF516_43385"
     CDS             <1..762
                     /locus_tag="VF516_43385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114674.1"
                     /translation="CGARLAHLSHFPRMEDGPAGPSKLFEHVLAQLMFNPRYKPIVAQ
                     CEEVVVHWDAPPDTCARLADRPVAALGELGALDSVRGVLAWRAMGAETRQLDPARASA
                     LPPAQRLVSFQCLMFDPAAQPAAPVERALALDGERFAVREARMKPLVGDGTSQIRLYK
                     QLAALMAPGFGYGRLTSNQAVGATPEEAALAETFRQPAFWLTRLDLLRDADLFLGNDW
                     LARRPDLLEVYPYHLHLQNFIEIFRRQYTQHAGRL"
     gene            759..1346
                     /locus_tag="VF516_43390"
     CDS             759..1346
                     /locus_tag="VF516_43390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114675.1"
                     /translation="MRAALVVLALCGVAHAEPGPTRAAYIADLIEALRGIDRATLANT
                     RKYIQIVERNKCQAPEMTLRVGCLLEAAGQNCKQLPVDARERCRRASDVIATNLLAER
                     VFVPNDVRYQIMSKQRDARSAIARELHRRYAALVAEFALSEVFPGPRADTAALATGID
                     RFCTDVAGTRDLSWQYCAAAIVWFVASDGAAEEAR"
     gene            1343..2494
                     /locus_tag="VF516_43395"
     CDS             1343..2494
                     /locus_tag="VF516_43395"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8114676.1"
                     /translation="MTKAHMAMLAAESRKLEQAGLYRPETIHPAHIVDFTSQDYLGLT
                     NDPRLVSAAQAALEHHGLGAGSARAFTGTREIHRDLEQAVARFLQVPDAIVLGSGYLA
                     NIGFYEALFDSRDCLFCDALVHPSTAEGIRLSSATAFPFHNNDVEDLEDKLRRSRSAR
                     FRAIATDGVFPFDGRVAELDGICSLAEKYDAMVVLDDSLGIGALGDTGRGARELRGMI
                     ARVDVVTGTFGKALGGAGGGFIAGRREVIEWLRQKATPYLFSIALAPPLAAAARAALD
                     LLERGEAPLATLRGHTAMMKQELEARGFRILGGEHPTLAVFVGGVVALQKMVNSLYER
                     GIHVHGLCYPVVPEGQARIRLEITARHSAQDISRAINAFDLAGRALGVI"
     gene            complement(2526..2963)
                     /locus_tag="VF516_43400"
     CDS             complement(2526..2963)
                     /locus_tag="VF516_43400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114677.1"
                     /translation="MPAFGGEAAGSPSLLALPARCAMSWPRVRSLKMHARRLNCTSRR
                     ALHVACSWSSLFATAFGGAGYCEVEMRLAACEALFAAGEHERGRAELREALHQIRIRA
                     EDINDPQWRRGYLTRNAENRRARELAEAWEVTDETAALLSDLV"
     gene            complement(3179..4186)
                     /locus_tag="VF516_43405"
     CDS             complement(3179..4186)
                     /locus_tag="VF516_43405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HEX8114678.1"
                     /translation="MSDNFEPLVEAARGGDEQAFRALVEPLGRELHAYAYRMLGGFHD
                     AADVLQESWLKAWRALATYEPRASFRAWMYRIVTNTCLDMLKARSRRVLPQDVSPSIA
                     PGPPTTAPRSDILWLEPYPDALLPAVPGPEEVARLREGIRLAFVRVVQVLPPRQRAAL
                     ILHDVLDWSVAEVAAILETTEAAINSALQRARAAIARPREESPALAPRHAEVIDRFVH
                     AWESGDFDDFVKLLATDAVMSMPPWEYWLDGKDAVVATMRSSGTWDGEPRPGRYRIVP
                     APMNGEPGALAYVRGPDGRYHAVCLTVMSLDADGRVVELTTFVLPELFAAWGFPPVLD
                     A"
     gene            4253..4894
                     /locus_tag="VF516_43410"
     CDS             4253..4894
                     /locus_tag="VF516_43410"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEX8114679.1"
                     /translation="MIKVFFLVSVSLDGYLAPPGMDMEHAGDPTYEDWAAQWGKLHSW
                     IFPQRFFRENLKLGEGGETGTDNQILEHTFRRTGVSIMGKRMFDLGERMWPEEAPFHT
                     PVFVLTSQVREPWVRPGGTTFYFVNDGIESALRQARAVAGDRDIRIAGGADAIQQYLR
                     AGLVDEFALAISPVMFGGGPRLFAGIGRDVGVELVETIASPRVTHVRYAVHKP"
     gene            complement(5020..>5218)
                     /locus_tag="VF516_43415"
     CDS             complement(5020..>5218)
                     /locus_tag="VF516_43415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114680.1"
                     /translation="SVQIILITCRPQEVLAADELCSPGQARRASADGRAHAVDMAAVI
                     RRFDVPRPATTARRSRELEEA"
BASE COUNT          790 a         1886 c         1783 g          759 t
ORIGIN      
        1 tgcggcgcgc gcctcgcgca cctgtcgcac ttcccgcgca tggaggacgg tccggccggc
       61 ccgagcaagc tgttcgagca cgtgctcgcg cagctgatgt tcaacccgcg ttacaagccg
      121 atcgtcgcgc agtgcgagga ggtcgtcgtg cactgggacg caccgccgga tacctgcgcg
      181 cggctcgccg atcgcccggt cgccgcgctc ggcgagctcg gcgcgctcga ctccgtgcgc
      241 ggcgtgctcg cctggcgcgc gatgggcgcc gagacccggc agctcgatcc cgcgcgtgcc
      301 agcgccctcc cgcccgcgca gcggctcgtc agcttccagt gcttgatgtt cgaccccgcc
      361 gcacagcccg cggcgcccgt cgagcgcgcg ctggcgctcg atggcgagcg gttcgcggtc
      421 cgcgaggcgc gcatgaagcc gctggtcggc gacggcacca gccagatccg gctctacaag
      481 cagctcgccg cgctcatggc gcccggcttc ggctatggcc gcctgaccag caaccaggcg
      541 gtcggcgcga cgcccgagga agccgcgctg gcggagacct tccggcagcc ggcgttctgg
      601 ttgaccaggc tcgacctgtt gcgcgacgcc gacctgttcc tcggcaacga ctggctggcc
      661 aggcgccccg atctgctcga ggtctacccg tatcacctgc acctgcagaa cttcatcgag
      721 atcttccggc gccagtacac gcaacacgcg gggcggctgt gagggcggcg ctcgtcgtgc
      781 tcgcgctgtg cggtgtcgcg cacgccgagc ctggccccac gcgtgccgcg tacatcgcgg
      841 acctgatcga agcgctccgt ggcatcgacc gcgcgacgct cgcgaacacg cgcaagtaca
      901 tccagatcgt cgagcgcaac aagtgtcagg cacccgagat gaccttgcgc gtcggctgcc
      961 tgctcgaggc cgcgggccag aactgcaagc agctcccggt cgacgcgcgc gaacgctgcc
     1021 gccgggcatc cgacgtgatc gcgaccaacc tgctggccga gcgcgtgttc gtgccgaacg
     1081 acgtgcgcta ccagatcatg agcaagcagc gcgacgcgcg ctccgcgatc gcgcgcgagc
     1141 tgcatcgtcg ctacgcggcg ctggtcgccg agttcgcgct gtccgaggtc ttccccgggc
     1201 cgcgcgccga caccgccgcg ctcgcgaccg ggatcgaccg gttctgcacc gacgtcgccg
     1261 gcacgcgcga tctgtcgtgg cagtactgcg cggcggcgat cgtgtggttc gtggcgagcg
     1321 acggcgcagc cgaggaggcg cgatgacgaa ggcacacatg gcgatgctcg ccgcggagtc
     1381 caggaagctc gagcaagccg ggctgtaccg gccggagacc atccaccccg ctcacatcgt
     1441 cgacttcacg agccaggact acctcggcct gaccaacgat ccgcgcctgg tgagcgccgc
     1501 gcaggccgcg ctcgaacacc acgggctcgg cgccggctcg gcgcgggcgt tcaccggcac
     1561 gcgcgagatc caccgcgatc tcgagcaggc ggtcgcgcgg tttctccagg tgcccgatgc
     1621 gatcgtgctc ggctcgggct acctcgccaa catcggcttc tacgaggcgc tgttcgacag
     1681 ccgcgactgc ctgttctgcg acgcgctcgt ccacccgagc accgccgaag gcattcgcct
     1741 gtcatcggcg accgcgttcc cgtttcacaa caacgacgtc gaggacctcg aggacaagct
     1801 gcggcggtcg cggtcggcgc ggtttcgcgc gatcgcgacc gacggcgtgt tcccgttcga
     1861 cggacgcgtc gccgagctcg acggcatctg cagcctggcc gagaagtacg acgcgatggt
     1921 cgtgctcgac gactccctcg gcatcggcgc gctcggcgac accgggcgcg gcgcccgcga
     1981 gctgcgcggc atgatcgcgc gggtcgatgt cgtcaccggc acgttcggca aggcgctggg
     2041 cggcgccggc ggcggcttca tcgcaggcag gcgcgaggtg atcgagtggc tacggcagaa
     2101 ggcgacaccg tatctgttct cgatcgccct cgcgccgccg ctcgcggcag cggcccgcgc
     2161 tgccctcgac ctcctcgagc gcggcgaggc accgctcgcc accctccgcg gccacaccgc
     2221 catgatgaag caggagctcg aggcgcgcgg cttccggatc ctcggtggag agcacccgac
     2281 gctcgcggtg ttcgtcggcg gcgtcgtcgc gctgcagaag atggtcaact cgctctacga
     2341 acgcggcatc cacgtccacg gcctgtgcta tccggtcgtc ccggagggcc aggcgcgcat
     2401 ccggctcgag atcaccgcgc gccattccgc gcaggacatc agccgcgcga tcaacgcctt
     2461 cgacctcgct ggccgcgcgc tcggcgtgat ctgatcgcca tgcggcggca cacctcgaac
     2521 gatgcctaca cgagatcgga gaggagcgcc gcggtctcat ccgtcacttc ccacgcctcg
     2581 gccagttccc gggcgcgacg attctccgca ttgcgagtga ggtagccccg tcgccactgg
     2641 ggatcgttga tgtcctcggc acgaatgcgg atctgatgga gggcctcgcg aagctcggcg
     2701 cggcctcgct cgtgttcgcc ggcggcgaac aaggcctcgc acgccgccag cctcatctcc
     2761 acctcgcagt aacctgcgcc gccgaacgcc gtcgcgaaca agctcgacca cgagcatgcg
     2821 acatgcagcg cacgtctcga tgtgcagttg agcctccgcg cgtgcatctt cagagagcgc
     2881 acccgaggcc agctcatcgc acaccgcgcg ggcagcgcca acaacgatgg cgagcctgcg
     2941 gcctcaccgc cgaacgccgg catgaccgcg gcgatggcga gccgccaggg agcacgaacc
     3001 gcgtcggatg gcccagcgcc gtagccgtgc ccacggactc gagacctcgc tggtgttgga
     3061 gcggccgcgc tgaggtgacg tagcccagag gggcgccgcc ggcgtcatgg gcgcagcggc
     3121 gcaccggcca gccccgatgc ctgaacgtcg gcaatcggtc ggatcgcttc cgcttcagct
     3181 acgcgtcgag caccggtggg aagccccatg ccgcgaacag ctccgggagc acgaacgtcg
     3241 tgagctcgac gacgcgaccg tcggcatcga gcgacatgac ggtgaggcag acggcgtggt
     3301 agcgaccatc gggtccgcgg acatacgcga gtgcgccggg ctcgccgttc atcggcgcgg
     3361 gcacgatccg gtagcgcccg gggcgcggct cgccgtccca cgtgcccgat gatcgcatcg
     3421 tcgccacgac cgcatccttg ccgtcgagcc agtactccca cggcggcatg ctcatcaccg
     3481 cgtcggtcgc gagcagcttg acgaagtcgt cgaagtcgcc gctctcccac gcgtgcacga
     3541 accggtcgat gacctcggcg tggcgcggcg cgagcgcggg tgactcctcg cgtgggcgcg
     3601 cgatcgcggc acgcgcacgc tgcagcgcgc tgttgatcgc ggcctcggtg gtctcgagga
     3661 tggcggcgac ctcggcgacg ctccagtcga ggacgtcgtg aaggatcagc gcggcgcgct
     3721 ggcgcggcgg cagcacctgc acgacgcgca cgaacgcgag ccggatgccc tcacgcagcc
     3781 gcgcgacctc ctcgggcccc ggcaccgccg gcaacagcgc atcggggtac ggctcgagcc
     3841 acaggatgtc ggagcgcggc gcggtcgtgg gtggccccgg cgcgatcgac gggctgacgt
     3901 cctgcggcag cacgcgacgc gagcgcgcct tgagcatgtc gaggcaggtg ttggtgacga
     3961 tccggtacat ccacgcgcgg aagctcgcgc gcggttcgta cgtcgcgagc gcgcgccacg
     4021 ccttgagcca tgattcctgg agcacgtcag cggcgtcgtg gaacccgccg agcatgcggt
     4081 aggcgtacgc gtgcagctcg cgaccgagcg gttcgaccag cgcacgaaac gcttgttcgt
     4141 cgccgccgcg ggcggcctcc accaagggct cgaaattgtc gctcatggac cgatgactgg
     4201 cgcgcccggc cgtcgtttca ccagcagacc acaccgcgaa aggcccgtac agatgatcaa
     4261 ggtgttcttc ctcgtcagcg tctccctcga cggatatctc gcacctcccg gcatggacat
     4321 ggagcacgcc ggcgacccga cctacgagga ttgggcggcc cagtggggca agctccacag
     4381 ctggatcttc ccgcagcgat tcttccggga gaatttgaag ctcggcgagg gcggcgagac
     4441 cggcaccgac aatcagatcc tcgagcacac gttccggcgc accggcgtca gcatcatggg
     4501 caagcgcatg ttcgacctcg gcgaacggat gtggcccgag gaagcgccgt tccacacgcc
     4561 ggtgttcgtg ctgacctcgc aggtgcgcga gccgtgggtg cggcccggcg gcacgacgtt
     4621 ctacttcgtc aacgacggca tcgagagtgc gcttcgccaa gcgcgcgcgg tggccggcga
     4681 tcgcgacatc cgcatcgccg gcggcgccga cgccatccag cagtacctac gcgccgggct
     4741 cgtcgacgaa ttcgcgctcg cgatctcgcc ggtgatgttc ggcggcgggc cgcgactgtt
     4801 cgcgggcatc ggccgcgacg tcggcgtcga gctcgtcgag accatcgcgt cgccgcgtgt
     4861 gacccacgtg cgctacgccg tgcacaagcc gtagccgcgg cactcgatcg cgcgcagtct
     4921 cggcgcacga tccatcgctg gaccctacgt acggcctcgc aaagcgccgt aggtgtgccg
     4981 ggttactctt gggggactga aagtcgggac aggccattgc tacgcctcct cgagctcgcg
     5041 cgaacgtcgc gccgtggttg ccggtcgcgg cacatcgaag cgacggatca ccgccgccat
     5101 gtcgaccgcg tgtgcccgtc catctgcgct cgcgcggcgc gcctgtccgg gactgcaaag
     5161 ctcatcggcc gcaagcacct cctgcggacg gcaggtgatc aggatgatct gaacgctc
//
LOCUS       DASVOR010002358         3152 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_230495,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002358 DASVOR010000000
VERSION     DASVOR010002358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230495"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1542)
                     /locus_tag="VF516_43420"
     CDS             complement(<1..1542)
                     /locus_tag="VF516_43420"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HEX8114681.1"
                     /translation="MRVPALLLTLTLAATATAEPRRIAFWPDTVPMTIQSHVDGAYVL
                     GAVRSLGKFHRVQGSPGFRAAADWLVGELKGRGLADAAVEHLPADGKTRYAHFPGYLG
                     WTASEGRLEEVVPRLRRLGDFATQPVALADYSQDADVSAELIDVGAGADAAAYQGKDV
                     RGKLVLADGPLPVVHRLAVEERGAAGIVSDFPNQRTAWSGQDPDLVRWGHLSPFQTAN
                     RFAFMISPRMAGELRRELAHGAVRLSAHVKAKMVPASFDVVSATIPGTDPAAGEIVLT
                     AHLCHELAGANDNASGSAALLGVARALRAAITDKTLPAPRRTIRLLWLPEIAGSQAWL
                     ISHPDLARNLRAGVHLDMVGGRPEITHAALHLSRTAGSLPHIVNEIARAWTADVDRAS
                     TEFAEKATGDGMIWPPGGRDALVTDLRPLELGSDHQVFEAFGVPMVYFHDWPDVTIHT
                     SKDVPDNLDATKLGRVAYMTAGIAWTLAALPDAEAARLVALEHAATDARIATARRTAL
                     DRGLAA"
     gene            complement(1562..2980)
                     /locus_tag="VF516_43425"
     CDS             complement(1562..2980)
                     /locus_tag="VF516_43425"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8114682.1"
                     /translation="MAGERVLVIDDSPTITKVVQLVLTKAGYEVQTAPDGEAGLAAVR
                     ASRPDLILLDFVMPKMNGYQFCRELTADAKLRDIPVILMSAKGDQVGERFVKVMGIVD
                     YITKPFSPEAITAVVQHTLGKYGPAGTVEEDPPSLVTGEDLAATDEADRAQARSAALA
                     QLRTSITEALAAQVALSDPGVAEDQAAAEAAVISRVATAALDDATLTRMLATVDLRLL
                     GDSAPGLRGDLRVVPLAEVLQLLDVQEQSGVLTVERSGARVDIYFRRGRVDQATATGV
                     PEEFLLGRFVLDAAVMQRADFEAFLESRTGQPKGAGQPKLIGQQLVKLGYLAEADLKT
                     CLVRQSSELIYEILRWRHGRFRLAAGAELPPSVIDAALGLDVEAVLMEGYRRVDEWHL
                     IERAIDNFDIVFLRNEDSVAQMGRGRLTREELAVLELVNGKNTVKEIIRKSRMGSFEV
                     SKMLYRLLSIKLIRRRVLPVAV"
BASE COUNT          430 a         1119 c         1137 g          466 t
ORIGIN      
        1 ggcggccagt ccgcgatcga gcgccgtgcg gcgggcggtc gcgatgcgcg cgtcggtcgc
       61 cgcgtgctcc agcgcgacca gccgcgcggc ctcggcgtcg ggcagcgccg ccagcgtcca
      121 cgcgatcccg gcggtcatgt acgcgacgcg gccgagcttg gtcgcgtcga ggttgtccgg
      181 gacgtccttg ctggtgtgga tcgtgacgtc gggccagtcg tggaagtaca ccatcggcac
      241 gccgaacgcc tcgaacacct ggtgatcgct gccgagctcg agcggccgca ggtcggtgac
      301 cagcgcgtcg cggcccccgg gcggccagat catgccgtcg ccggtggcct tctcggcgaa
      361 ctcggtcgag gcgcggtcga cgtcggcggt ccacgcccgg gcgatctcgt tgacgatgtg
      421 cggcagcgag ccggccgtgc gcgacaggtg cagcgccgcg tgcgtgatct cgggccggcc
      481 gccgaccatg tcgaggtgga cgccggcccg caggttgcgc gcgaggtcgg ggtggctgat
      541 cagccaggcc tgcgagccgg cgatctcggg cagccagagc aggcggatcg tgcggcgcgg
      601 cgccggcagc gtcttgtcgg tgatcgccgc gcgcagcgct cgcgccacgc cgagcagcgc
      661 cgcgctgccc gacgcgttgt cgttggcgcc ggcgagctcg tggcacaggt gcgcggtcag
      721 cacgatctcg cccgccgccg gatcggtgcc cgggatcgtc gccgagacca cgtcgaagct
      781 cgccggcacc atcttcgcct tgacgtgcgc ggacagccgc acggcgccgt gcgcgagctc
      841 gcggcgcagc tcgccggcca tgcgcggcga gatcatgaac gcgaaccgat tggcggtctg
      901 gaacggcgac aggtggcccc agcgcacgag atcggggtcc tgccccgacc acgcggtgcg
      961 ctggttgggg aagtcggaca cgatgccggc ggcgccgcgc tcctcgacgg cgagccggtg
     1021 gaccacgggg agcggcccgt cggccagcac cagcttgccg cgcacgtcct tgccctggta
     1081 cgcggcggcg tcggccccgg ccccgacgtc gatcagctcg gcgctgacgt cggcgtcctg
     1141 gctgtagtcg gcgagcgcga ccggctgggt cgcgaagtcg cccagccggc gcagccgggg
     1201 cacgacctcc tcgagccggc cttcgctcgc ggtccagccc aggtagccgg ggaagtgggc
     1261 gtagcgggtc ttgccgtcgg ccggcaggtg ctcgacggcg gcgtcggcga gcccgcggcc
     1321 cttgagctcg ccgaccagcc agtccgccgc ggcgcggaac cccggcgagc cctgcacgcg
     1381 gtggaacttg cccagcgagc ggaccgcgcc gagcacgtac gcgccgtcga cgtggctctg
     1441 gatcgtcatc ggcacggtgt cgggccagaa cgcgatccgg cgcggctcgg cggtggccgt
     1501 tgccgcgagc gtcagggtga gcaggagggc gggcacgcgc atgcccggag cttatgcccg
     1561 gtcacaccgc gaccgggagc acgcggcgcc ggatcagctt gatcgacagc aggcggtaca
     1621 gcatcttcga cacctcgaac gaccccatcc gcgacttgcg gatgatctcc ttgacggtgt
     1681 tcttgccgtt gaccagctcg agcacggcga gctcctcgcg ggtcagccgg ccgcgcccca
     1741 tctgggccac cgagtcctcg ttgcgcagga acacgatgtc gaagttgtcg atcgcgcgct
     1801 cgatcaggtg ccactcgtcg acccggcggt agccctccat cagcaccgcc tcgacgtcca
     1861 gcccgagcgc ggcgtcgatc acgctgggcg gcagctcggc gcccgcggcc aaccggaacc
     1921 ggccgtggcg ccagcgcagg atctcgtaga tcagctcgct cgactggcgg accaggcagg
     1981 tcttgaggtc ggcctcggcg aggtagccga gcttgaccag ctgctggccg atcagcttgg
     2041 gctggccggc ccccttgggc tggccggtcc gcgactccag gaacgcctcg aagtcggcgc
     2101 gctgcatcac ggcggcatcg agcacgaacc ggccgagcag gaactcctcc ggcacgccgg
     2161 tcgccgtcgc ctggtcgacc cgaccgcggc ggaagtagat gtcgacccgt gcgccggagc
     2221 gctccacggt gagcacgccc gattgctcct ggacatcgag cagttgcagg acctcggcca
     2281 gcggcaccac gcggagatcg ccgcgcagcc ccggggcgct gtcgcccagc aggcggaggt
     2341 cgaccgtggc cagcatgcgg gtgagcgttg cgtcatcgag cgcggcggtc gcgacccggc
     2401 tgatcacggc cgcctcggcc gccgcctggt cctcggcaac gcccggatcc gacagggcaa
     2461 cctgggcggc gagggcctcg gtgatcgagg ttcggagctg tgcaagtgcc gcactgcgcg
     2521 cctgggccct gtccgcctcg tcggtggccg ccaggtcttc cccggtgacc agggacggcg
     2581 ggtcctcctc caccgttccg gccgggccgt atttccccag cgtgtgctgg acgacggcgg
     2641 tgatcgcctc cggggagaac ggcttggtga tgtagtcgac gatgcccatc accttgacga
     2701 agcgctcgcc gacctgatct cccttcgcgc tcatcaagat cactggaata tcgcggagct
     2761 tggcgtcggc tgtcagctcg cgacagaact ggtacccgtt catcttgggc atcacgaagt
     2821 cgagcaggat cagatccggg cgcgatgccc ggaccgccgc cagcccagcc tcgccgtccg
     2881 gcgcggtctg aacctcgtac ccggccttgg tcagcacgag ctgcaccacc ttggtgatgg
     2941 tgggactgtc gtcgatcacg aggacccgct cgccggccat gtcggtatat ccctcactcc
     3001 gcgggacatt tcgccggagg gatcctcatc ctatgtcgtc cgatccgtgc cagcaatcga
     3061 cacgcttcct gaattggttg tggtttgccc accgtggtac ggttagcccg cgtggcgctc
     3121 ctcgagggag acctggagca ttaccccagg aa
//
LOCUS       DASVOR010002359        20101 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_346619,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002359 DASVOR010000000
VERSION     DASVOR010002359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20101)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20101)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20101
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346619"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /locus_tag="VF516_43430"
     CDS             complement(<1..926)
                     /locus_tag="VF516_43430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114683.1"
                     /translation="MKTPLAAMFLLACHGGAASTPDGGGGTGSDGGGGTPASGWSHET
                     LINHPPSFIDYDLSASAVDINASGIAVALWREQPRPTGMNLLWANVYRAGVWGAPLAI
                     ADPGSSEGAVSVLPSGDAMVVYVQPPSIQAKRYVLATGTWTAATQLSVDPAAGSSPRI
                     AADRLGNAIAIWIQGDQIWARRYDASAGWAATVTQLSASPRLVYVPEITVDGNNRFTA
                     VWVEDSAPFDPSLPGGGPDKATAHARRYVTDWEVDQRVGWAPTDLLGDFDSAGRIAVD
                     AGRAGSVFVVWEQFHTQPDQSLQLAIDAARFDP"
     gene            1092..6554
                     /locus_tag="VF516_43435"
     CDS             1092..6554
                     /locus_tag="VF516_43435"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX8114684.1"
                     /translation="MRKIGVILAALWVLGCGGKKSAGELTVTGSPAGQVSGPVRIILT
                     FSRPMVAKDELNQPVGRAPLAFVPDVPREARWTDVETLVVVPTATLPVSTRFTVVVPG
                     DTAARDGSSLGKDTSFEFSTERLTLIAEAVGSKERAAKHPLVRLTFNQQVAFDQAAAS
                     CSYVANGQTAKVKLAPDTAAGPAKDYSVIPDGDLAPDTDWVLTCKAGLMGVVGNLGLE
                     AAAEAKFHTYGPLRYVDMDPSGNDIVPDEKLRLRLAFSNPLAEPYKLSIKPPVAGFPQ
                     GCHALSGAAVGVSCSGQFEALTDYTLTVDGSQQDVFGQALGKPQVIELRTADALPTIS
                     LESGYFVAELKRAVVPMWTRNVTSVDVVAVPVTQASFHELAPLLDWWDPKPADFSKTK
                     LLRPITSKIKIDGTKNHWGQHSLDPAAITGGTPGSGMYYLELGSAEVQKGAFADGGKK
                     KVLVNFTDIGVVSKLSPTRGMVWATKLTTGKPLPGATVTVRDATGKVTYTGTTDSSGT
                     AALPGVEALGATSESADGLRIYVQLQADWTMINPTASGGLSTWAFNVSSDRGTAPTRL
                     RGFMHTDRGLYRPGDKVHVKGLARVTRLGAPLALPDQSKDPSKRVAVQVDGPQGKSFL
                     TAKAKLSPFGGFWFDVDLPPDARLGDYTITATLEHGTFTRSFTVEEYRPATFEVTGKT
                     KESFVVNRGDVHGTVSANYFYGAPVRNGSVDVIVHSRRRRVAFDQLPGFDFGDERNYE
                     SYTMYESDDSQHMVTEDHVALDGKGSGAFTFSVNPDEVTSDSDLLVHADVHAPSNEVI
                     SKSFTIPYFRSRRYFGIQSPGYFLDVKRPQKFQIVSVGPDGKIVDGAAQVKVTRRDWN
                     CVWEDWGYRGSYQCKDTTQTILDTRIQIAGKPAEIELTPATGGEYLVEVEGGKDTAPA
                     AQRVYAWGDEGGSWRSDDTLAFKIATDKQEYKAGDTANLILETDLANATGLVTIERDG
                     VIDQRLIDVTPTTKRLTVPITASYAPNVYVSVALVQGRTGDGPRGKPRMRMGIANLSV
                     RPQDNRLTVTIDSDKKEYRPGDQVTATVKVTDTQGQPALAEVSITAADEGVLSLVAYK
                     TPDPVPTFYAPWGLGVQSATQLEYIRDIPAPNVERPATGGDSVGTVRSRFVASAVWVP
                     GAVTDAGGVATVTFKAPDNLTAFRMMAVAADKATRFGSADKRFTVGKPLQLHSALPRF
                     VDLGDALQAGVVVHNETGKAGTATVKLVADEHVTVASGAEQTVKVPVGGRVPVLFDLT
                     PAASGTAALTFSVAMNGETDAVKLDLPVRHPGNIKTLDVEHGATKDAKIISLNLPPDA
                     VPATAQVVISVDPDGLSGIEEGLGDLIGYPYGCMEQTTSKMVAMIAARDLAESLAIDG
                     LTGDRLDGFVKAGVAKINKQQTAYGGFSLWPGGEPNAFYTAYGLWGLYMAKQAGYPVD
                     PSRIEEAIQYLQNDGANPDKNAPVYNEMGNLAAQAFATYVRALYKDKSAAAAATTLLA
                     QRGLPIYGKVYAARALAVGVGPKDPAVVKVVEELATLANAATKTDALIKEPNEKDHDY
                     YMSSDTRTTSAVLLGLVELEPKHPAIKPLVRTLMAARRATHYWDTHANFYSLLALTTY
                     AKAFAGSSPSVTVQLAGKDLVTGTLAGKQKLRVVTAPLPAQADLTIAPNGEVSYSVAV
                     RYRARPETIQAESHGIELTREYQGDDGKPKTEFHVGDVVVVKLHVKVPDDSTHVMVSD
                     ALPAGFEALNTRLATVAADTTKQNRIWWGEHREIHDERVDFASEYVWNGSFEYTYSIR
                     AIATGKFARPPATAQLMYDPKTTAQTALDILEVKAK"
     gene            6554..8839
                     /locus_tag="VF516_43440"
     CDS             6554..8839
                     /locus_tag="VF516_43440"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HEX8114685.1"
                     /translation="MRKLWEVVRSRRLRWPRRIAIGLAVPVVIAALAWHIAIRAADVP
                     HNLLAKEPASSLTVVDAQGQLLRQEATSAGTKERWVPLDRISPYLVAATLASEDNDFS
                     DHAGVDWTAVARAAWLDLRGGGFGGSTITMQLVRLLAGTPRSLSGKLRQMVLAGRLER
                     MRSKQEILEQYLNRVYYGQGAWGIEQAAQLYFGKHAADLALGEAALLAVLPRGPTSYD
                     PFRNAGRVMARRTHILELMAKHDRITAGERALAERVPLDLDHHKPSFRAPHFVELVKQ
                     RLPPDLRHAASVRTTLDLPLQRAVETALDVHLDHVGGRHIRQAAVIAMRNSDGAILAM
                     VGSRDYHDDASNGAFDGVTARLRPGSTLKPFVYGAAFERGDTPASIALDLVLPEDVHE
                     SYSTDVKSHGFARYRESLAGSYNLSAVHTLQRVGVATVLRKLRTAGITTLDRPDDAYD
                     WGLAIGHAEVRLIDLTSAFSFIGRAGRPIAPRAVEVATTADGRRWTEPVVERPPVFSE
                     EIAYLLWDILSDPDARKPMFGDRVPLELPFKVALKTGTTKAYTDLWAIGVTREYTVGV
                     WAGNFDGSPTYHVMSTEGATPLVRAVYTAIAARFGAPTEPARPPTIVAADICPLSGKL
                     PGPHCEHHKRELFIADHLPEETCDWHQVVCGQPAVVYPPQIRPWARYYGKPDPPVCTV
                     ASANAPLAITAPANGAHFIIEPYRAATSQRPLLAASPADPALRWTIDGVPADRWVPTP
                     GTHRVIATRGNVSDEVTITYE"
     gene            complement(8900..9850)
                     /locus_tag="VF516_43445"
     CDS             complement(8900..9850)
                     /locus_tag="VF516_43445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114686.1"
                     /translation="MSGRSLVLFSILAGCGGDGRVGHLPDAPPPPDAPLADTPSGSPP
                     DGASSYDPTGGIVTPGEHGGAGCPDSDETTFTLAAGAVTSRPFAGIVTGAAGNGVFYV
                     RGTGNQELFGTIPTDANGNYNVTVPLFCGAQLVKLHWANATCAYDIVYSVTTDTCTDR
                     DIRITLGWDDLGIDWELHLIKPGGKINDNATDCTWTSCIGTAPDWGVIGSTADDPSKD
                     VDNTGHYGPENILLSGPEAGTYTVMVEHWNGSGSPLSDGTVILNVAGKPAVVQRMMDL
                     APYHVWTAATITFPGGVVTPSSAVHDCSANWTNGCRDSIP"
     gene            complement(10311..11405)
                     /locus_tag="VF516_43450"
     CDS             complement(10311..11405)
                     /locus_tag="VF516_43450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114687.1"
                     /translation="MLRTYRGVVERCFAPAELTHRVHLAGLTIELQGLPFQQQDQGVA
                     ACATTAVWSALTKVIRTDGGRAPTPFAVTLAATDHRVQGRAFPALAGLDLDQMATAIH
                     EFGYQPHVFQRIDESDDFFLALKTYLRSGIPVVVRARVLDGDLHALTLVGFRERKRES
                     TSPNGDLQIGNSNRMKLRSRGIERWYAHDDRLGAYARLGFEERHTKLKLLPSEAGYEH
                     LETPMVFSDAIVPLYSKLRLTAEELVGFAVDLSPTVRFLAQDEDNIYVDPRFMLGGEY
                     LRQLHGRSIERNRIIEICTTLALSRYVGVVSWFHGDAWLLDAIYDTTDLRRGDVSRPP
                     LLALVPYNARWIASLEQIRALRFGDGPLIG"
     gene            complement(11488..11652)
                     /locus_tag="VF516_43455"
     CDS             complement(11488..11652)
                     /locus_tag="VF516_43455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114688.1"
                     /translation="MVEHPYVDRHWLEEYAGYYATLFDPPRPKATRLHFFDVSWTFDE
                     FKKQLDSAIA"
     gene            complement(11822..11989)
                     /locus_tag="VF516_43460"
     CDS             complement(11822..11989)
                     /locus_tag="VF516_43460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114689.1"
                     /translation="MATDPPKLSENTTPVITPERAKELVREGEEAARAYRQRVERMWT
                     ISKDARQTRAR"
     gene            12539..15154
                     /locus_tag="VF516_43465"
     CDS             12539..15154
                     /locus_tag="VF516_43465"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HEX8114690.1"
                     /translation="MVAAVVPATVEGNVCIDVTLDAGTHVVPLGDGSAPVRFYVEPGT
                     RVTGTLALVREPAGVRLPRVELGCSPPLRLYNPRQSLQPAHTRLGRLGDWLVDLLVDL
                     KVNGILVDERGELGLRFRPQVLRALTLKEQSQFNDNAAQRVPLLIAPETASAPDAPGV
                     LHLAPLGLGGPMLTITGAVSTDGVPGDPSRVVVSHTGSLELQGAGLRLALAGGPGATA
                     PSITVHGDADLELERAQLTLTVNGGMHFAAAEVTRAQLRLKAVELPSFGKARLRIDEP
                     VTIQLEGKDLHFDAGERALSGHLVAKVKLARPSGGELERTIGVRLGSGRASFDAPSGV
                     IDELLDAVAGFTVRRARIPPADVVAAGASGSAELRARIQDRTGAVPRGGNRVELLVDG
                     VRSRPCRLALIERARESICLQALIFKQDRASQEHADALIAAARRGVRVRVIVDALGNI
                     ERIGEVAEGKAIYKKLRDGGVELRLFNDPEQTGLRPLIDAVAEDPDLAEIGDLSAIGR
                     RLRQPHTVLRLLTRLMDVVKGRVPASQASRQRVRDGLTRMARASCASHPTADQAGGDR
                     RDLSELVYLLHVAATLNRRWHEKHLIADGQRAILGGMNVADEYLFGGDPAHPEPVIDM
                     GVARPAWRDTDVLIEGPAAADAYQAFARNWLTVTGETLPPPSPPATPVAGSTHVQIVQ
                     HRPGEDGDHHTAAVLIEALATLKAGQRALISNAYFLPTAALTHYRQALARAARRGVDV
                     RVLTNSRESTDEAAICDAAIYPYRELAAAGVKLFERTSTRTMHQKVAVLGGSTCLIGS
                     WNSDNRSETFNSEVMAVICCEELARQMEAQFEADCCVDGPEPSARPILAAQLEHHSPA
                     EAWMSLWAALQSELL"
     gene            complement(15427..16077)
                     /locus_tag="VF516_43470"
     CDS             complement(15427..16077)
                     /locus_tag="VF516_43470"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX8114691.1"
                     /translation="MATPYPPTLPARTRTSGLAIAGFVCSLFCGVLGLIFSILGRNEC
                     KRSGGTVGGEGLALAGIIISCVTLALNLAVVPAVAIPAFMDYTKRSKKTESALQLNKL
                     GKNAKRAYIETGSFPKGNAPLTPPNPCCGQPNNHCRAVPEMYAADPVWRALDFQIDVP
                     TLFQYSYEASPDGQSFVAKAVGDLDCDTTFITYELRGTAANGNPSMTLIEPPPNSD"
     gene            16324..17625
                     /locus_tag="VF516_43475"
     CDS             16324..17625
                     /locus_tag="VF516_43475"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEX8114692.1"
                     /translation="MRTRPLFTILIAAALTAGAATGCEKKSGTAGAGSAAGKATSPSG
                     AASAKPIVVGSTLSLSGAFAATGQIHKIAGDEFLDRLNKAGGLLGRKVEWVVRDDESD
                     QAKVATLYEQLISQDKVDLIMGPYATPNILSAMAVAERHGYVLPQHTSVLAPLMKYPC
                     QFPGWSHGFAPNEFVPGRLFDAMASLPRPPRKVAILTNQNGSTDFVSYGAGDNKKGFV
                     SIAKERGLEVVAEIKYPPGAAANWAPLATQVRDAKPDLVINSGLGIDPVNLLQAMAQL
                     NYSPPLMFSLFPAPGPLLKLGPAADRILAVSLFEPNQAILDKLGPVATEIVKDFKNRA
                     TEAKLPYTVFETQAASSWNAWEILVSGVKAAGSVDNKAICDALHKAGADTTFSGHLTF
                     DPAQNNFWPPTTSGLKQIQDGDWVTVWPAERAAAPIKPPPG"
     gene            17843..18712
                     /locus_tag="VF516_43480"
     CDS             17843..18712
                     /locus_tag="VF516_43480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEX8114693.1"
                     /translation="MKFLQAALTGMLLGGLHALMAAGLSLSWGVLRIINLAHFGLILI
                     GAYLTFQIASSWHIDPILTLVVTVPALFAAGAVLQWGFDRLAVTELNSLLISFGLLVI
                     LVQAVSNLWTADFQQMTTDVNRYATGSLSAGRLVFPSTTLIAFALSVLLLGVGHQALQ
                     RTFIGRALRAFAMDRTVAAAFGIDHRRISILLAGIAGGSAAVAGMLFALSTALTPFAA
                     FEWFGIVFAVVILGGIGNVVGTLLAGLLVGALSSLASVIHSPATEPFVVFSLIVVALL
                     VRPQGLFARAGGH"
     gene            18793..>20101
                     /locus_tag="VF516_43485"
     CDS             18793..>20101
                     /locus_tag="VF516_43485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8114694.1"
                     /translation="MLSLYRESLGLPVSYLSLLTLVLVWTIQATSWNILSGFSGYFSF
                     GQAAYVGTGAYATAVLYGRHGVSFYVTVVVAAGLCALLALAVGGLAFRLRSLRGEIFA
                     LLTLAVPFILGALARTSRAIDGGQGIPLPVPTFPAQIGLFQDFLYLVGLLVAAGAVWT
                     AFAIAHSRFGWALAAIRDAEHVAEGLGVATFQYKLLALVASAVIGGVGGSVFALYIGF
                     VTVESVFNLNFPLFVIVMSVLGGRTHWLGPVIGAVLVVLLQNRLASSSFQSWQLIALG
                     AILVVLVAVAPEGLTSGVRRRRWPVLGALFGTALILWLARAPGQPLDWILTGMLVAAA
                     VAVWPGRRRRALSTEPAAVEPSALRSSASGTPTSDPAAPPPHPAPATPAVLVDCRKVA
                     RYFGGVHALEDVTLRIHEGEIVGLVGPNGSGKTTLVSLLSGSLR"
BASE COUNT         3117 a         6971 c         6910 g         3103 t
ORIGIN      
        1 gggtcgaatc gcgcggcatc gatcgctagc tggaggctct ggtcgggctg ggtgtggaac
       61 tgctcccaga ccacgaacac cgagccggcg cggccggcat cgaccgcgat ccgtccggcg
      121 gagtcgaagt cgccgagcag atcggtggga gcccagccga cgcgctggtc gacctcccag
      181 tccgtgacgt agcggcgggc gtgcgcggtc gccttgtccg gcccgccgcc ggggaggctg
      241 gggtcgaacg gcgcgctgtc ctcgacccac accgccgtga agcggttgtt gccgtcgacg
      301 gtgatctccg ggacgtagac gagccggggg gaggcgctca gctgggtcac ggtcgccgcc
      361 cagccggcgc tcgcgtcgta gcgacgggcc cagatctggt cgccctggat ccagatcgcg
      421 atcgcgttcc ccagccggtc ggcggcgatg cggggagaac tgccggcggc cggatcgacg
      481 ctgagctgcg tggcggcggt ccaggtgccg gtggcgagca catagcgctt cgcctggatg
      541 ctcggcggct ggacatagac gaccatcgca tcgccgctgg gcaggaccga gaccgctccc
      601 tcgctcgacc ctgggtccgc gatggcgagc ggggcgcccc agacaccagc gcggtacacg
      661 ttggcccaca gcaggttcat gccggtgggc ctgggctgct cgcgccatag cgcgacggcg
      721 atgccgctcg cgttgatatc gaccgcggac gccgagaggt cgtagtcgat gaagctgggc
      781 gggtgattga tcagcgtctc atggctccag ccgctcgccg gggttcctcc gccgccatcg
      841 ctgccggtac cgccgccgcc gtccggcgtg ctcgcggcgc cgccgtggca tgccaggagg
      901 aacatcgccg ccaggggggt cttcaagcgc ctggtcttca cggttgcacc tcgctcggtc
      961 ggggccagca acaccaccgc gccggggatc gaagcacgct ttcaccacgc cgtccacctg
     1021 gcgtgagcag gcgcaaaagc gatcggccgc gcgccggatc gccccggtgg ggcctgatac
     1081 ggttggtggt gatgaggaag atcggtgtga tcctggccgc gctgtgggtg ctcggttgcg
     1141 gcggcaagaa atccgcaggt gaactgacgg tcaccggctc tccagccggc caggtctcgg
     1201 gcccggtgcg gatcatcctc acgttctcga gaccgatggt cgcgaaggac gagctgaacc
     1261 agccggtcgg ccgggcgccg ctcgcgttcg tgcccgacgt cccgcgcgaa gcgaggtgga
     1321 ccgacgtcga gacgctcgtc gtcgttccga ccgcgacgct gccggtgtcg acgcggttca
     1381 ccgtcgtcgt gcctggcgat accgccgcgc gcgacggcag ctcgctcggc aaggacacga
     1441 gcttcgagtt ctcgaccgag cggctgaccc tgatcgccga ggcggtcggc agcaaggagc
     1501 gcgcggcgaa gcacccgctg gtccggctga cgttcaacca gcaggtcgcg ttcgatcagg
     1561 ccgcggcgag ctgcagctac gtcgcgaacg gccagaccgc caaggtcaag ctcgcgcccg
     1621 acaccgccgc cgggcccgcg aaggactact cggtcatccc cgacggcgac ctcgcacccg
     1681 acaccgactg ggtgctgacc tgcaaggcgg gcctgatggg ggtcgtcggc aacctcggcc
     1741 tcgaggccgc cgcggaggcg aagttccaca cctacggacc gctgcgctac gtcgacatgg
     1801 atcccagcgg caacgacatc gttcccgacg agaagctgcg gctgcggctc gcgttctcga
     1861 acccgctcgc cgagccctac aagctctcga tcaagccgcc ggtcgccggg ttcccgcagg
     1921 gctgccacgc gctctccggt gcagcggtcg gcgtcagctg ctcgggccag ttcgaggcgc
     1981 tgaccgacta cacgctgacc gtcgacggct cacagcagga cgtgttcggc caggcgctcg
     2041 gcaagccgca ggtgatcgag ctccgcaccg ccgacgcgct gccgacgatc tcgctcgagt
     2101 cgggttactt cgtcgccgag ctcaagcggg cggtcgtccc gatgtggacc cgcaacgtca
     2161 cgtcggtcga cgtcgtcgcg gtgccggtca cccaggccag cttccacgag ctcgccccgc
     2221 tgctcgactg gtgggaccca aagcccgccg acttctcgaa gacgaagctg ctgaggccga
     2281 tcacgagcaa gatcaagatc gacggcacga agaaccactg gggccagcac tcgctcgatc
     2341 cggccgcgat caccggcggc acaccgggct cgggcatgta ctacctcgag ctcggctcgg
     2401 ccgaggtcca gaagggcgcg ttcgccgacg gcggcaagaa gaaggtgctc gtcaacttca
     2461 cggacatcgg cgtcgtgagc aagctgtcgc cgacccgcgg catggtgtgg gcgaccaagc
     2521 tcaccaccgg caagccgctg cccggtgcga cggtgaccgt gcgcgacgcg accggcaagg
     2581 tcacgtacac cggcacgacg gacagctctg ggaccgcggc gctgcccggc gtcgaggcgc
     2641 tcggcgcgac cagcgagagc gccgacggcc tgcgcatcta cgtccagctg caagccgact
     2701 ggacgatgat caacccgacg gcgagcggtg ggctgtcgac gtgggcgttc aacgtctcgt
     2761 cggatcgcgg caccgcgccg accaggctgc gcgggttcat gcacaccgat cgcggcctgt
     2821 accggccggg cgacaaggtc cacgtcaagg gcctcgcgcg cgtcaccagg ctcggcgcgc
     2881 cgctcgcgct gcccgatcag agcaaggacc cgagcaagag agtggcggtc caggtcgacg
     2941 gcccgcaggg caagtcgttc ctgaccgcga aggcgaagct gtcgccgttc ggcgggttct
     3001 ggttcgacgt cgatctgccg cccgacgcgc ggctcggcga ctacacgatc accgccacgc
     3061 tcgagcacgg gacgttcacg cggtcgttca ccgtcgagga gtaccggccc gcgacgttcg
     3121 aggtcaccgg caagaccaag gagagcttcg tcgtcaaccg cggcgacgtc cacggcacgg
     3181 tctcggcgaa ctacttctac ggcgcgccgg tgcgcaacgg cagcgtcgac gtgatcgtcc
     3241 acagccgccg gcgccgcgtc gcgttcgatc agctccccgg cttcgacttc ggcgacgagc
     3301 gcaactacga gtcgtacacc atgtacgagt ccgacgactc gcagcacatg gtcaccgagg
     3361 accacgtcgc gctcgacggc aagggcagcg gcgcgttcac cttctcggtc aaccccgacg
     3421 aggtcacgag cgactccgat ctgctcgtgc acgcggacgt ccacgcgccg tcgaacgagg
     3481 tgatcagcaa gtcgttcacg atcccgtact tccgatccag gcgctacttc ggcatccagt
     3541 cgccgggcta cttcctcgac gtcaagaggc cgcagaagtt ccagatcgtc tcggtcggac
     3601 ccgacggcaa gatcgtcgac ggggccgcgc aggtgaaggt cacccgccgc gactggaact
     3661 gcgtgtggga ggactggggc tatcgcggct cgtatcagtg caaggacacg acccagacga
     3721 tcctcgacac caggatccag atcgcgggca agccggccga gatcgagctc acgccggcga
     3781 ccggcggcga atacctcgtc gaggtcgagg gtggcaagga caccgcgccg gccgcgcagc
     3841 gggtgtacgc gtggggcgac gagggcggct cgtggcggag cgacgacacg ctcgcgttca
     3901 agatcgcgac cgacaagcag gagtacaagg ccggcgacac cgcgaatctc atcctggaga
     3961 ccgacctcgc gaacgcgacc ggcctggtca cgatcgagcg cgacggcgtg atcgaccagc
     4021 gcctgatcga cgtcacaccg acgaccaagc ggctgaccgt gccgatcacc gcgagctatg
     4081 cgccgaacgt gtacgtctcg gtcgcgctcg tgcagggccg caccggcgac ggtccgcgcg
     4141 gcaagccgcg gatgcggatg ggcatcgcga acctgagcgt gcgcccacag gacaaccggc
     4201 tgacggtcac gatcgacagc gacaagaagg agtaccggcc gggtgaccag gtgacggcca
     4261 ccgtgaaggt caccgacacg cagggccagc cggccctggc cgaggtctcg atcaccgccg
     4321 ccgacgaggg cgtgctgtcg ctggtcgcgt acaagacgcc cgatccggtg ccgacgttct
     4381 acgcgccatg gggcctcggc gtgcagagcg cgacccagct cgagtacatc cgcgacatcc
     4441 ccgcgccgaa cgtcgagcgc ccggcgaccg gcggtgactc ggtcggcacg gtgcgctcgc
     4501 ggttcgtcgc gagcgcggtg tgggtgcccg gcgcggtcac cgacgccggc ggcgtcgcga
     4561 ccgtgacgtt caaggcgccc gacaacctca ccgcgttccg catgatggcg gtcgccgccg
     4621 acaaggcaac ccggttcggc tcggccgaca agcggttcac ggtgggaaag cccctgcagc
     4681 tgcactcggc gctgccgcgg ttcgtcgacc tcggcgacgc gctgcaagcc ggcgtggtcg
     4741 tccacaacga gaccggcaag gccgggaccg ccacggtcaa gctcgtcgcc gacgagcacg
     4801 tcacggtcgc cagcggcgcc gagcagacgg tcaaggtccc ggtcggcggg cgcgtgccgg
     4861 tgctgttcga tctcacgccc gcggcgagcg gcacggcggc gctgacgttc tcggtcgcga
     4921 tgaacggcga gaccgacgcg gtcaagctcg acctgccggt gcgccacccc ggcaacatca
     4981 agaccctcga cgtcgagcac ggcgcgacga aggatgccaa gatcatctcg ctgaacctgc
     5041 cgcccgacgc ggtgccggcg accgcgcagg tcgtgatctc ggtcgatccc gacgggctgt
     5101 cgggcatcga ggagggcctc ggcgatctga tcggctaccc gtacggctgc atggagcaga
     5161 cgacatcgaa gatggtcgcg atgatcgcgg cgcgcgacct cgccgagtcg ctcgcgatcg
     5221 acgggctcac cggcgaccga ctcgacggct tcgtcaaggc gggtgttgcg aagatcaaca
     5281 agcagcagac cgcgtacggc gggttctcgc tgtggccggg cggcgagccg aacgcgttct
     5341 acaccgccta cggcctgtgg ggcctctaca tggcgaagca ggccggctac ccggtcgatc
     5401 cgtcccggat cgaggaggcg atccagtacc tccagaacga cggcgcgaac cccgacaaga
     5461 acgcgccggt ctacaacgag atgggcaacc tcgcggcgca ggcgttcgcg acctacgtgc
     5521 gcgcgctgta caaggacaag agcgcggcgg cggccgcgac cacgctgctc gcccagcgcg
     5581 gcctgccgat ctacggcaag gtctacgcgg cgcgcgcgct cgcggtcggc gtcgggccga
     5641 aggacccggc ggtggtcaag gtcgtcgagg agctcgccac gctcgcgaac gccgcgacca
     5701 agaccgacgc gctgatcaag gagcccaacg agaaggacca cgactactac atgtcgagcg
     5761 acacgcggac gacgagcgcc gtgctgctcg gcctcgtcga gctcgagccg aagcacccgg
     5821 cgatcaagcc gctcgtgcgc acgctgatgg cggcgcggcg cgcaacccac tactgggaca
     5881 cccacgcgaa cttctactcg ctgctcgcgc tgaccacgta cgcgaaggcg ttcgccgggt
     5941 cgtcgccgtc ggtgacggtc cagctcgccg gcaaggacct cgtgaccggc acgctcgccg
     6001 gcaagcagaa gctgcgggtc gtcaccgcgc cgctgccggc ccaggccgat ctgacgatcg
     6061 cgccgaacgg cgaggtgtcc tacagcgtcg cggttcgcta ccgcgcgcgg ccggagacga
     6121 tccaggccga gtcccacggc atcgagctga cgcgcgagta ccagggcgac gacggcaagc
     6181 cgaagaccga gttccacgtc ggcgacgtcg tggtcgtcaa gctgcacgtc aaggtccccg
     6241 acgactcgac ccacgtcatg gtctccgacg cgctgcccgc cgggttcgag gcgctcaaca
     6301 cgcggctcgc gaccgtcgcc gccgacacga cgaagcagaa caggatctgg tggggcgagc
     6361 accgcgagat ccacgacgag cgggtcgact tcgcgtcgga gtacgtgtgg aacggctcgt
     6421 tcgagtacac gtactcgatc cgtgcgatcg cgaccggcaa gttcgcgcgc ccgccggcga
     6481 ccgcgcagct gatgtacgac ccgaagacca ccgcgcagac ggcgctcgac atcctcgagg
     6541 tcaaggcgaa gtagtgcgga agctgtggga ggtcgttcgc agccgccggc tgcgctggcc
     6601 gcgccggatc gcgatcgggc tcgcggtgcc ggtggtgatc gcggcgctcg cgtggcacat
     6661 cgcgatccgc gcagccgacg ttcctcacaa cctgctcgcg aaggagccgg ccagctcgct
     6721 gaccgtcgtc gacgcgcagg gccagctgct gcgccaggag gcgacctcgg ccggcacgaa
     6781 ggagcgctgg gtcccgctcg atcggatctc gccgtacctc gtcgcggcga cgctcgcctc
     6841 ggaggacaac gacttctccg accacgcggg tgtcgactgg acggcggtcg cacgcgccgc
     6901 gtggctcgac ctgcgcggcg gcgggttcgg cggctcgacg atcacgatgc agctcgtgcg
     6961 gctgctcgcc ggcacgccgc gcagcctgtc cggcaagctg cgccagatgg tgctcgccgg
     7021 ccggctcgag cgcatgcggt ccaagcagga gatcctcgag cagtacctga accgcgtcta
     7081 ctacggccag ggcgcgtggg gcatcgagca ggcggcgcag ctctacttcg gcaagcacgc
     7141 cgccgatctc gcgctcggcg aggcagcgct gctcgccgtc ctgccgcgcg ggccgacgtc
     7201 gtacgatccg ttccgcaacg ctgggcgggt gatggcgcgt cgcacgcaca tcctcgagct
     7261 catggccaag catgatcgga tcaccgccgg cgaacgcgcg ctcgccgagc gcgtcccgct
     7321 cgatctcgac caccacaagc cgagcttccg ggcgccgcac ttcgtcgagc tggtcaagca
     7381 gcgcctgccg cccgacctcc ggcacgccgc gtcggtgcgc accacgctcg acctgccgct
     7441 gcagcgcgcg gtcgagaccg cgctcgacgt ccacctcgat cacgtcggcg ggcgccacat
     7501 ccggcaggcc gccgtgatcg cgatgcgcaa ctccgacggc gcgatcctcg cgatggtcgg
     7561 ctcgcgcgac taccacgacg acgcgagcaa cggcgcgttc gacggtgtca ccgcgcggct
     7621 gcggccgggc tcgacgctca agccgttcgt ctacggcgcg gcgttcgagc gcggcgacac
     7681 cccggcgtcg atcgcgctcg atctggtgct gccggaggac gtccacgagt cgtactcgac
     7741 cgacgtcaag agccacggct tcgcgcgcta ccgcgagtcg ctcgccggct cgtacaacct
     7801 gtcggcggtc cacacgctgc agcgggtcgg cgtggcgacc gtcctgcgca agctgcgcac
     7861 cgccgggatc acgacgctcg accgccccga cgacgcgtac gactggggcc tcgcgatcgg
     7921 ccacgccgag gtccgcctga tcgacctgac gagcgcgttc tcgttcatcg gccgcgccgg
     7981 ccgcccgatc gcgccgcgcg ccgtggaggt cgcgacgacc gccgacggcc gccgctggac
     8041 cgagccggtc gtcgagcgcc cgccggtgtt ctccgaggag atcgcctatc tgctgtggga
     8101 catcctcagc gatcccgacg cgcgcaagcc gatgtttggt gaccgcgtgc cgctcgagct
     8161 gccgttcaag gtcgcgctga agactggcac gacgaaggcg tacaccgatc tgtgggcgat
     8221 cggcgtcacc cgcgagtata cggtcggcgt gtgggccgga aacttcgacg gctcgccgac
     8281 ctaccacgtg atgtcgaccg agggcgcgac gccgctcgtg cgcgccgtgt acaccgcgat
     8341 cgccgcgcgg ttcggcgcgc cgaccgagcc ggcacgcccg ccgacgatcg tcgccgccga
     8401 catctgcccg ctatcgggca agctgccggg tccgcattgc gagcaccaca agcgcgagct
     8461 gttcatcgcg gaccacctgc cggaggagac ctgcgactgg catcaggtcg tgtgcggcca
     8521 gcccgcggtc gtctatccgc cgcagatccg gccgtgggcc cgctactacg gcaagcccga
     8581 tccaccggtg tgcacggtcg ccagcgcgaa cgcgccgctc gcgatcaccg caccggcgaa
     8641 cggggcgcac ttcatcatcg agccgtaccg cgccgcgacc tcgcagcgcc cgctgctcgc
     8701 ggcctcgccc gccgatccgg cgctgcgctg gacgatcgac ggcgtccccg ccgaccgctg
     8761 ggtgcccacg cccggcacgc atcgcgtgat cgcaacgcgc ggcaacgtca gcgacgaggt
     8821 cacgatcacc tacgagtgac ccgacgcctt gcgcaggatc ggctcgaccg cgtcatgagc
     8881 cgacagtcgg ccgcgtcgcc taggggatcg agtcgcggca gccgttcgtc cagttcgccg
     8941 agcagtcgtg caccgccgag ctcggggtga cgacgccgcc ggggaacgtg atcgtcgcgg
     9001 cggtccacac gtggtacggc gcgaggtcca tcatgcgctg cacgacggcc ggcttgccag
     9061 cgacgttgag gatcacggtg ccgtcgctca gcgggctgcc gctgccgttc cagtgctcga
     9121 ccatcaccgt gtacgtccca gcctcgggcc cgctgagcag gatgttctcg gggccgtagt
     9181 ggccggtgtt gtcgacgtcc ttgctcggat cgtcggcggt gctgccgatc acgccccagt
     9241 cgggcgcggt gccgatgcac gaggtccacg tgcagtcggt cgcgttgtcg ttgatcttcc
     9301 cgccgggctt gatcaggtgc aactcccagt cgatgccgag gtcgtcccag cccaacgtga
     9361 tacggatgtc gcgatccgtg caggtgtccg tcgtgacgct gtagacgatg tcgtacgcgc
     9421 acgtcgcgtt cgcccagtgc agcttgacga gctgcgcgcc gcagaacagc ggcaccgtga
     9481 cgttgtagtt gccattcgcg tcggtcggga tcgtgccgaa cagctcctgg ttgccggtgc
     9541 cgcgcacgta gaataccccg ttgcctgccg cgcccgtgac gatgccggcg aacggccgac
     9601 tcgtgacggc gcccgcggcg agcgtgaatg tcgtctcatc ggagtcgggg cagccagcgc
     9661 caccgtgctc gccgggcgtg acgatgccgc cggtcgggtc gtacgacgac gccccatcgg
     9721 gcggactgcc cgacggcgtg tcggcgagcg gtgcgtcggg cggcggcggt gcatcgggga
     9781 gatgaccgac ccttccgtca ccgccgcagc cggcgaggat cgagaacagc accagggagc
     9841 gtccgctcat gccccatcgt accgcggccg ggtgcagctc caccagccag tgggaaaggc
     9901 gagccttgcg atggtcgcgg tgacgcggcc ggccgtgcgt ggggcagatc ccatgcggtt
     9961 cgtttcggta ccggttgcgc ccacggtgta gcgatggctg ggcaggcgcc gtagcggcac
    10021 agccgcttgt tcgagacacg ctgactgcgg atgcagtccc gggcaaaccg cgaccaggac
    10081 cgcgccagct acggatcacg tcgtcgctcc ggccgatgca gggctagaag accaggatcg
    10141 cgacgtcgcg ttggttgttc tcgccgccac agttgacctg cgccagcacc atcatgccga
    10201 gcttgcgtcc tccgatcacg acttcgatcg gggcgccacc cacgcggggc aagcgtccct
    10261 ccgcggatag ctggctcgac gatgcgtacc ttcgtgagtt cgctaccagg tcagccaatc
    10321 agagggccat cgccgaatcg tagcgctcgg atctgctcga gcgaggcgat ccagcgcgcg
    10381 ttatacggca cgagggcaag caacggcggg cgcgacacgt caccgcgccg cagatccgtg
    10441 gtgtcgtaaa tcgcatcgag cagccaagcg tcaccgtgaa accagctgac gacgccgaca
    10501 tagcgggaca acgcgagcgt ggtgcagatc tcgatgatcc gattgcgctc gatggaccgg
    10561 ccatgaagct ggcgaaggta ttctccgccg agcatgaagc gcggatcgac ataaatgttg
    10621 tcctcgtctt gcgcgaggaa tcggaccgtg ggtgagaggt cgactgcgaa tccgacgagc
    10681 tcttccgcag tcagccgaag cttcgagtat agcggtacga tggcatccga gaataccatc
    10741 ggtgtctcga ggtgctcgta gcctgcttcc gatggcagca gcttcaattt ggtatgtcgt
    10801 tcctcgaagc caagccgggc gtatgcaccc aatcgatcat cgtgagcata ccaccgctcg
    10861 atgccgcgcg atcggagctt catccgattg ctgttcccga tctggagatc accattcggg
    10921 ctcgtgcttt ctctcttccg ttctcggaag cctactagcg tgagtgcgtg aagatcgccg
    10981 tcgaggacgc gcgcgcgaac gacgaccggg atcccggagc gcagatacgt cttgagggcc
    11041 aggaaaaagt cgtcggattc atcaattcgc tggaatacat gaggctggta gccgaactcg
    11101 tggatcgcag tggccatctg atcaaggtcg agcccggcga gcgcggggaa cgcgcgccct
    11161 tgcacacgat gatccgtcgc agccagggtc acggcgaacg gtgttggtgc ccgcccaccg
    11221 tcggtgcgga tcaccttggt caacgcagac cacacggcag tggtcgcgca cgcggcgacg
    11281 ccctgatcct gctgctggaa cgggaggccc tgaagctcga ttgtcaagcc tgcgaggtgc
    11341 acgcgatggg taagctcggc tggcgcgaag cagcgctcca caacaccgcg gtacgttcgc
    11401 agcacggtgc gtccgatcgg cgctgcgggc agtggacgaa ttacggaaaa accaagatag
    11461 tgcgtcgcga tccggtcacc attgccatta tgcgatcgcg gagtcgagtt gcttcttgaa
    11521 ctcatcgaac gtccagctca catcgaagaa gtgcagccgg gttgccttgg gtcgcggcgg
    11581 atcgaacagc gtggcataat agccggcgta ctcttcgagc cagtgccgat cgacatacgg
    11641 gtgctcgacc aagatcgtct tcgcactgat ttctggatgg ctcaagtacg atgcgaggta
    11701 tcggacctga tcgcacggcg gtgccgctga acccccgggt gtacagctgg cctcacggat
    11761 gcaatcggcg agctgttcga accgctcaca tcggagcgac agacgctgag gcacgacgtg
    11821 gtcaccgagc gcgggtctgc cgagcgtcct tggagatcgt ccacattcgt tcgacgcgct
    11881 ggcgataggc gcgagcggct tcctctccct cgcgtacgag ctccttggcg cgctctggcg
    11941 tgatcaccgg cgtcgtgttc tcgctgagct tgggcggatc tgtagccatg gggtacgtag
    12001 gtagagcaga cctcaacggc gcgcgcaacg caactcgcag atgggtgggt ggtatgaagg
    12061 aggatccgac atcacgcatg ttctgcagga ttctcgttac ttggccccta catcgaacgt
    12121 tcaccgggca tggagcaggt gatcggtcag gtcgacccag gcctgcagcg cggccctgac
    12181 ccgcgtgcat acttacggag ctgctcgccg gttcccgtat cgggcttgcg gctcgagtcg
    12241 tcccacttgc gtgggaggtc gacagcttgc caggccgaga gcccgacact gcgcaggcgg
    12301 agaaggcgca cgaccgtcca cgagttcgga tcgcggcgcc gcgtcatccc tccagcctga
    12361 cccacgcgcg cgatcgagct gccgatgggc ggttacactt ctagcgtacg gatcgtggcg
    12421 atacaggtga acttgttggg ccccagtggt agtcggcgcc aaatcgtgat ccgacacttg
    12481 cgtccagaac tcgcgtggcc atactacaaa gtcaggtaca aggggaaggg gtggggagat
    12541 ggtcgctgcc gttgttccgg ccacggtcga aggcaacgtt tgtatcgatg tcacgctcga
    12601 cgcgggcacg cacgtggtgc cgctgggtga cggaagcgct ccggtgcgct tctacgtcga
    12661 gccgggcacg cgggtcacgg gcacgctcgc gctcgtccgt gaacctgcgg gcgtgcggct
    12721 gcccagggtg gagctcggct gctcgccccc gctgcgcctg tacaacccca ggcagtcgct
    12781 gcagcccgcg cacacgcgcc tgggccgcct gggcgactgg ctggtcgacc tgctcgtcga
    12841 tctgaaggtg aatggcatcc tggtggacga gcggggtgag ctgggcttgc gcttccgccc
    12901 gcaggtcctg cgcgcgctga cgctcaagga gcagtcgcag ttcaacgaca acgcggcgca
    12961 gcgcgtgccg ctcctcatcg cacccgagac ggcctctgcg ccggacgcgc ccggcgtgct
    13021 gcacctcgcg ccgctcgggc tgggcgggcc gatgctcacc atcacgggcg ccgtgagcac
    13081 cgatggcgtg cccggcgatc cctcgcgcgt cgtggtctcg cacaccggca gcctcgagct
    13141 ccagggtgcg ggcctgcgcc tggcgctcgc gggcgggccc ggggcgaccg cgcccagcat
    13201 caccgtgcac ggcgacgccg atctcgagct cgagcgcgcc cagctgaccc tgaccgtgaa
    13261 cggcgggatg cacttcgccg ccgccgaggt cacgcgcgcc cagctgcgcc tcaaggcggt
    13321 cgagctgccg agcttcggca aggcgcggct acgcatcgac gagccggtca cgatccagct
    13381 cgagggcaag gacctgcact tcgacgcggg cgagcgcgcg ctgtccgggc acctggtcgc
    13441 caaggtgaag ctcgcccggc cctcgggcgg cgagctcgag cgtaccatcg gcgtgcgcct
    13501 cgggtccggc cgcgcctcgt tcgacgcacc ctcgggcgtg atcgacgagc tgctcgacgc
    13561 ggtggcgggc ttcacggtgc ggcgggccag gatcccgccc gccgatgtcg tcgccgcggg
    13621 cgcctcgggc agcgccgagc tgcgcgcccg catccaggac cggaccggcg ccgtcccgcg
    13681 cgggggcaac cgcgtggagc tgctggtcga cggcgtgcgc tcgcgcccgt gccggctcgc
    13741 gctgatcgag cgcgcgcgcg agtcgatctg cctgcaggcg ctcatcttca agcaggaccg
    13801 cgcgagccag gagcacgccg atgcgctcat cgcggcggcg cggcgcggcg tgcgcgtgcg
    13861 cgtgatcgtc gatgcgctcg gcaacatcga gcggatcggc gaggtggccg agggcaaggc
    13921 catctacaag aagctgcgcg acggcggcgt cgagctgcgc ctgttcaacg atcccgagca
    13981 gaccggcctg cgcccgctga tcgacgcggt ggccgaggat cccgatctcg ccgagatcgg
    14041 cgatctcagc gcgatcggcc gccggctcag gcagccgcac acggtgctgc gcctgctcac
    14101 ccgcctcatg gacgtggtca agggccgcgt gcccgccagc caggcgtcgc ggcagcgcgt
    14161 gcgcgacggc ctgacccgga tggcgcgcgc gtcctgcgcc agccatccga ccgctgacca
    14221 ggccggcggt gaccggcgcg atctgtccga gctggtgtac ctgctgcacg tggccgccac
    14281 gctcaaccgc cgctggcacg agaagcacct catcgccgac gggcagcgcg ccattctggg
    14341 cggcatgaac gtcgccgacg agtacctgtt cggcggcgac ccggcccacc ccgagcccgt
    14401 catcgacatg ggcgtggcgc ggccggcctg gcgcgacacc gacgtgctca tcgaagggcc
    14461 cgcggccgcc gacgcctacc aggcgttcgc gcgcaactgg ctcaccgtga ccggcgagac
    14521 gctgccgccg cccagcccac cggcgacgcc ggtggcgggc agcacccatg tgcagatcgt
    14581 gcagcaccgc ccgggtgagg acggcgatca ccataccgcg gcggtgctga tcgaggcgct
    14641 ggcgacgctc aaggccggcc agcgcgcgct gatctccaac gcctacttcc tgcccaccgc
    14701 ggcgctcacc cactaccggc aggcgctggc gcgcgcggcc aggcgcggcg tcgacgtgcg
    14761 cgtgctgacc aactcgcgcg agtccaccga cgaggcggcg atctgcgacg cggccatcta
    14821 cccgtaccgc gagctggccg ccgccggggt caagctgttc gagcgcacga gcacgcgcac
    14881 catgcaccag aaggtggcgg tgctgggcgg cagcacgtgc ctcatcggct cgtggaacag
    14941 cgacaaccgc tcggagacct tcaactccga ggtcatggcg gtcatctgct gcgaggagct
    15001 ggcccggcag atggaggcgc agttcgaggc cgactgttgc gtcgacgggc ccgagccgag
    15061 cgcgcggccc atcctggcgg cgcaactcga gcaccactcg ccggccgagg catggatgag
    15121 cctgtgggcc gcgctgcagt ccgagctgct ctgactcgtc gagaagtcgc cctcgaacag
    15181 ccgcgcccgc ccgtcgccga tctcgtcgcc gagcacgcgg cgaaacacac cgtcgccgtg
    15241 gccggaccgc acgcctgcac ctcaccgaac acgaacccag gcgtattcct cagtactgca
    15301 tgggctcgat ttgctggttc atggataacc tattttcaag gaatatcctg atgcgtcagg
    15361 tgtcagcttt cacgttactg gcatggtgga ccgtgaccgt gccccgcgat cgagtgcgcg
    15421 cgtatcctag tccgagttcg gcggaggctc gatgagcgtc atcgacggat ttccgttcgc
    15481 ggctgtccct ctcagctcgt acgtgatgaa cgtggtgtcg cagtcgagat cgccgaccgc
    15541 cttggcgacg aagctctggc cgtcggggct cgcctcgtag gagtactgga acaacgtcgg
    15601 cacatcgatc tggaagtcga gggccctcca gaccggatcg gcggcgtaca tctcaggcac
    15661 cgcccggcaa tggttgttcg gctggccgca gcacgggttc ggtggggtca gcggcgcgtt
    15721 gcccttcggg aacgagccgg tctcgatata cgcgcgcttg gcgttcttgc cgagcttgtt
    15781 gagctggagc gccgactcgg tcttcttcga ccgcttcgtg tagtccatga atgcggggat
    15841 cgcgacggcg gggacgacgg cgaggttgag cgcgagcgtg acgcaagaga tgatgatgcc
    15901 cgcgagcgcg agcccctcgc cgccaaccgt gccgcccgag cgcttgcact cgttgcggcc
    15961 caggatcgag aagatcaggc cgagcacgcc gcagaacaac gagcacacga agccggcgat
    16021 cgccaggcct gaggtccggg tgcgggctgg caacgtgggc gggtagggag tggccatcgg
    16081 gcccgactct atcatgcaac cgcgagcgca ccgcacgttc ggccgatcgc ctgcctaccc
    16141 ggccggcccg acctctgcac cgccaccccg gagcgcagcg tcggccagcc gtgctggcgg
    16201 aacagggggg ccggatgggt gaactcgctt gcgcgtcgag cggaatgcgg catatgctgc
    16261 gcgcgacatg agggtccacc aggccgtgta aaggaccgca tcctccaaca tcgagtctca
    16321 cacatgcgca ctcgcccgct ctttaccatc ctcatcgctg ctgcgctgac ggccggcgcg
    16381 gcgactggct gcgaaaagaa gtcgggtacg gcaggtgccg gctccgctgc cggcaaagcg
    16441 acgtcaccct ctggggcggc gagcgcaaag ccgatcgtgg tcggctcgac gctttccctg
    16501 agcggggcct ttgcggccac cggtcagatc cacaagatcg ccggtgacga gttcctcgac
    16561 cgcctgaaca aggccggggg gctgctcggc cgcaaggtgg aatgggtcgt ccgtgacgat
    16621 gagtcggatc aggcgaaggt cgccaccctg tacgagcagc tcatctcgca ggacaaggtc
    16681 gacctcatca tgggccccta cgcgaccccg aacattctct ccgcgatggc agtggcggag
    16741 cggcacggat acgtcctccc gcagcacaca tccgtgctcg caccgctcat gaagtatccg
    16801 tgccagtttc cgggatggtc gcacgggttc gcgcccaacg agttcgtgcc cggccggctg
    16861 ttcgacgcga tggccagcct ccccaggccg cccaggaagg tcgccattct caccaatcag
    16921 aacggctcga ccgacttcgt gtcgtacggt gcgggcgaca acaagaaggg cttcgtctcc
    16981 atcgccaagg agcgcggcct cgaggtcgtc gccgagatca agtacccacc cggcgcggcg
    17041 gccaactggg cgcctctggc cacccaggtg cgggacgcga aacccgatct ggtgatcaat
    17101 agcggcctgg ggatcgatcc ggtcaatctc ttgcaagcga tggcgcagct caactactcg
    17161 ccgccgctca tgttcagcct gttcccggca cctggcccgc tcctcaagct cgggcccgcc
    17221 gcggaccgca tccttgccgt gtccctgttc gagcccaatc aggccatcct ggacaagctc
    17281 ggtccggtgg cgaccgagat cgtgaaggac ttcaagaacc gcgccaccga ggccaagctg
    17341 ccgtatacgg tgttcgagac tcaggcggcc tcttcctgga acgcctggga gatcctcgtg
    17401 tcgggggtca aggcggcggg cagcgtcgac aacaaggcga tctgcgacgc gctgcacaag
    17461 gctggagccg acacgacgtt cagcggccac ctgacgttcg atccggccca gaacaacttc
    17521 tggccgccga ccacatcggg gctcaagcag atccaggatg gagactgggt cactgtctgg
    17581 cctgccgaac gggctgccgc gccgatcaag ccgccgccag gctgagcgcc aggccgagtg
    17641 ccaggccgag tgccaggccg agtgaaagag tcagggtcct cggctgtgct accgtgtggc
    17701 cgcccgcggt cggcgccatg aacccagaag accactgtgt gaagggcctt gccgacggcg
    17761 agggcaatct ggggccgctg cgagcaggcg ccccctggcc gggcctgggt cgcacggcag
    17821 cgatccccga cggtggtctt gcatgaagtt cttgcaggcg gcgctgaccg gcatgctgct
    17881 gggtggcctg cacgctctca tggcggcggg tctgtcgctc tcgtgggggg tgctccggat
    17941 catcaatctc gctcatttcg gactcattct catcggcgcc tacctcacgt tccagatcgc
    18001 ctcgtcgtgg cacatcgacc cgatcctcac cctcgtcgtc acggttccgg ctctgtttgc
    18061 cgccggtgcc gtcctccagt ggggtttcga tcggctcgcg gtgaccgagc tcaactcgct
    18121 tttgatcagc ttcgggctcc tggtcatcct ggtccaggcg gtgagcaacc tctggacggc
    18181 ggacttccag cagatgacga ccgacgtgaa ccggtacgcc accggctcgc tctcggccgg
    18241 ccgtctggtg tttccctcga ccacgctcat cgccttcgcc ctctcggtcc tcctcctcgg
    18301 ggtgggccac caggctctcc agcgcacgtt catcggccgg gcgctccgcg ccttcgccat
    18361 ggaccgcacg gtcgccgcgg cgttcggcat cgatcaccgc cgcatctcca tcctgctggc
    18421 cggcatcgcg ggcggctcgg cggccgtggc cgggatgctg ttcgccctgt cgaccgcgct
    18481 gactccgttc gcggcgttcg agtggttcgg catcgtgttc gccgtggtca tcctgggcgg
    18541 gatcggaaac gtcgtgggga cgctcctggc cggcctgctc gtgggggcac tgtccagtct
    18601 ggcctcggtc attcactcgc ccgccaccga gccgttcgtg gtcttctcgc tcatcgtcgt
    18661 cgcgctcctc gtccgaccgc aggggctgtt tgcgcgcgcg ggtggccatt gagggcgcgg
    18721 ccatcgaggg cgagggcgag cgcgttcgcc acgggcctgt gggcatttcc gctggcgacc
    18781 gcagggctcg cggtgctctc tctgtaccgg gagagtctcg gccttccggt ctcctacctg
    18841 tcgctcctca ccctcgtgct ggtctggacc atccaggcca ccagctggaa catcctgtcc
    18901 gggttcagtg ggtatttcag cttcggccag gcggcctacg tggggaccgg tgcgtacgcg
    18961 acggcggttc tctacggccg ccatggggtg agcttctacg tcaccgttgt cgtggcggcc
    19021 gggctgtgcg cgctcctcgc gctggcggtc gggggcctgg cgttccggct gcgctccttg
    19081 cgcggcgaga tcttcgccct cctcaccctc gcggtgccgt tcatcctcgg tgcgctggcg
    19141 cggaccagcc gcgcgatcga cggcggacag ggcatcccgc tcccggttcc gacctttccg
    19201 gcccagattg gcctgttcca ggactttctc tatctcgtgg gtctcctggt cgcggccgga
    19261 gcggtctgga cggccttcgc catcgcccat tcgcgatttg gctgggccct cgccgcgatc
    19321 cgcgatgccg agcatgtcgc cgagggcctg ggggtggcca cgttccagta caagctcctg
    19381 gcgctggtgg ccagtgcggt gatcggcgga gtgggcggga gcgtcttcgc gctctacatc
    19441 ggattcgtca cggtcgagag cgtcttcaat ctgaactttc cgctgttcgt gatcgtgatg
    19501 agcgtgctgg gcggacggac gcactggctg gggccggtca tcggcgcggt gctggtcgtc
    19561 ctgctccaga accggctggc ctcgtccagt ttccagtcct ggcagctgat cgcgctgggg
    19621 gcgatcctgg tcgtcctcgt cgcggtcgcc ccggaggggc tcacgagcgg tgtgcgcagg
    19681 cggcggtggc cggtcctggg ggcgctgttc ggcacggccc tgatcctgtg gctggcgcgc
    19741 gcgccgggcc agccgctcga ctggatcctg accggcatgc tcgtggcggc ggccgtggcg
    19801 gtctggcccg ggcgcaggcg gcgggcgctg tcgaccgagc cggcggccgt cgagccctcg
    19861 gcactccggt cttccgcatc cgggacgccg acaagcgatc cggccgcgcc accaccgcat
    19921 cccgctccgg ccaccccggc cgttctggtc gactgccgca aggtggcccg ctacttcggc
    19981 ggtgtccacg cgctcgagga cgtcaccctg cgcatccacg agggcgagat cgtgggtctc
    20041 gtgggcccga acggatccgg caagacgacc ctggtcagcc tgctgtccgg gtcgcttcga
    20101 c
//
LOCUS       DASVOR010002360         2008 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_552549,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002360 DASVOR010000000
VERSION     DASVOR010002360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552549"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..279
                     /locus_tag="VF516_43490"
     CDS             61..279
                     /locus_tag="VF516_43490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2997 domain-containing protein"
                     /protein_id="HEX8114695.1"
                     /translation="MHKQDIEIVINAKGEVTFQVKGVKGGSCIAETKFLEQALGGDTA
                     IVDQQKTSEFYEGSEGYVSTWAGEGKDD"
     gene            298..945
                     /locus_tag="VF516_43495"
     CDS             298..945
                     /locus_tag="VF516_43495"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8114696.1"
                     /translation="MSNPTSSGPGNEPGNDPSADQLTDELARKYDPERLLRMLSKRAG
                     KGERLDHSIRQKYEKRFGVDLGHVRIYTGEFAEEFNRQRGAYAVTVGSTGMILMGGSP
                     EKSMASASGQALLAHELTHVAQAQKGLHRKATSAGGMPFAQEHEAEAEAVEQSVAQSG
                     GSHDSTNAAANAASAEVASREAMTRAIEKIKERVQEMAAEAARLQMVRNGVQRRA"
     gene            1054..1734
                     /locus_tag="VF516_43500"
     CDS             1054..1734
                     /locus_tag="VF516_43500"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEX8114697.1"
                     /translation="MTDTSADAGVVQSRVQLERLIAEDELAGRTITGFAAPSVGLRGV
                     DLDGVTMERLDLRGADGEDVRLERAELKQCDLRTSQWRGALWHRVRAKDCDLTELDLS
                     SAQLLRCDLGPVRMARIRFHLAKIQNTTFNQSELYSADFAGAVLIKVVFDGYEHATVS
                     LSRTDWTGAALFDVDFRRANMYGATLRNAALVRCNLKGVNLCNADLTGARLVGCETTG
                     VDLDGATF"
     gene            1744..>2008
                     /locus_tag="VF516_43505"
     CDS             1744..>2008
                     /locus_tag="VF516_43505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114698.1"
                     /translation="MPSRPDLREIASNVKDAFADADRETLLDVLTFVIKEYVVEGPPP
                     MLIHQAETLSDLRAPTFAQLIAALQTRFDHPELSMFVVDGEQVS"
BASE COUNT          349 a          691 c          700 g          268 t
ORIGIN      
        1 ggatcgatcc gcctggtcgt tcgcaagtgg gtctcggagt agcgaggcca ggaggccacc
       61 atgcacaagc aagacatcga gatcgtgatc aacgccaagg gcgaggtgac gttccaggtc
      121 aagggcgtca agggcggcag ctgcatcgcg gagaccaagt tcctcgagca ggcgctcggc
      181 ggcgacaccg cgatcgtcga ccagcagaag acgtccgaat tctatgaggg ttcggagggt
      241 tacgtgtcga cctgggccgg ggaaggcaag gacgactgac cgcgtagaaa gacgggcgtg
      301 agcaatccaa catcctccgg accgggcaac gagccgggca acgacccgag cgccgaccag
      361 ctcaccgacg agctcgcgcg caagtacgac ccggagcgcc tgctccgcat gctgtccaag
      421 cgcgccggca agggcgagcg gctcgaccac tcgatccgcc agaagtacga gaagcggttc
      481 ggggtcgacc tcggccacgt ccggatctac accggcgagt tcgccgagga gttcaaccgc
      541 cagcgcggcg cgtacgccgt cacggtcggc tcgaccggca tgatcctgat gggcggctcg
      601 ccggagaagt cgatggccag tgcgtccggc caggcgctgc tcgcccacga gctcacgcac
      661 gtcgcccagg cccagaaggg cctgcaccgc aaggcgacgt cggcgggcgg gatgccgttc
      721 gcccaggagc acgaggccga ggccgaggcg gtcgagcagt cggtggccca gtccggcggc
      781 agccacgaca gcaccaacgc ggccgcgaac gccgcgagcg ccgaggtcgc ctcccgggaa
      841 gcgatgaccc gcgcgatcga gaagatcaag gagcgggtcc aggagatggc ggccgaggca
      901 gcgcgcctcc agatggtgcg caacggcgtc cagcgccgcg cctgatcgtc catttcggca
      961 tcccggctcc atcccgggcc gtcccggcgg tctggccgct tccggtgcga cggggccgac
     1021 gtcccggcgg atctcggggt atcctcgagg gaaatgaccg acacaagcgc cgatgccggc
     1081 gtcgtgcaat cccgcgtcca gctggagcgg ctgatcgccg aggacgagct cgccggccgg
     1141 acgatcaccg gcttcgcggc cccgtcggtc gggttgcgcg gcgtcgatct cgacggcgtg
     1201 accatggagc ggctcgacct gcgcggcgcc gatggcgagg acgtccggct cgagcgcgcc
     1261 gagctcaagc agtgcgacct gcggacctcg cagtggcgcg gggcgctgtg gcaccgcgtc
     1321 cgcgccaagg actgcgacct gaccgagctc gacctgagct cggcgcagct cttgcgctgc
     1381 gacctcggcc cggtgcggat ggcccggatc cggttccacc tggccaagat ccagaacacg
     1441 acgttcaacc agtccgagct gtactcggcc gacttcgccg gcgcggtgct gatcaaggtc
     1501 gtgttcgatg gctacgagca cgccacggtg tcgctgtcgc gcaccgactg gaccggcgcg
     1561 gcgctgttcg acgtcgactt ccggcgggcc aacatgtacg gcgcaacgct gcgcaacgcc
     1621 gcgctcgtgc gctgcaacct caagggcgtg aacctgtgca acgccgacct gaccggcgca
     1681 cggctggtcg gctgcgagac caccggcgtc gacctcgacg gagcgacatt ctaggagaac
     1741 accatgccgt cccgacctga cctccgcgag atcgccagca acgtcaagga cgcgttcgcc
     1801 gacgccgatc gcgagaccct gctcgacgtc ctgaccttcg tgatcaagga gtacgtcgtc
     1861 gaggggccgc cgccgatgct gatccaccag gccgagaccc tgagcgacct gcgcgcgccg
     1921 acgttcgccc agctgatcgc cgcgctgcag acccggttcg atcaccccga gctgtcgatg
     1981 ttcgtggtcg atggcgagca ggtcagcg
//
LOCUS       DASVOR010002361         1650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_55861,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002361 DASVOR010000000
VERSION     DASVOR010002361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55861"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1284
                     /locus_tag="VF516_43510"
     CDS             <1..1284
                     /locus_tag="VF516_43510"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEX8114699.1"
                     /translation="PLDGMENQGTHLLGALLDPLGNEPGYHPNVISSAVTADGQAVTV
                     QFDGSAALRSGDHRGADPFFNGMILTGSEGAKVRLTVSRGGYDVAFYKLEGLGPTGWA
                     NLCDHDGDDAVPLAGKWQRSGFHETAPDRISFACTGAVAYKCLVWGYLAGSDSTSLGW
                     RAHQACTRMARGDYCANGHSHTREGTTITIYDFAGVTSPPPLHFDGVVDWAPNPNRMF
                     FEAAWSDGAHPASCLSRLRWQSLPLGPLCDDGDLRDPRLDTGVRFCEDIAWPPPGSEP
                     TGALLFNASRYTDMALHVWQRGDDMVSTVQGFYQLPNVIQPFPSVGPYEHIRNDGSVL
                     RSLRSDQDRASYEDLWLYRNGSDFVVAGASRPPPGFTPGSFEGFAAKQQSPGMLPFNL
                     YIHQDTGDTLSTTGTPDAAYVLQWTIGYVAPPETR"
BASE COUNT          242 a          606 c          568 g          234 t
ORIGIN      
        1 ccgctcgacg gcatggagaa ccagggtacg cacctgctgg gggccctcct cgaccccctc
       61 ggcaacgagc cgggctatca ccccaacgtg atcagctcgg cggtgaccgc ggacggccag
      121 gccgtgaccg tgcagttcga cggcagcgcc gcgctgcgat cgggcgacca ccgcggcgcc
      181 gacccgttct tcaacggcat gatcctgacc ggctccgagg gcgcgaaggt ccggttgacc
      241 gtgtcgcggg gcggctacga cgtcgcgttc tacaagctcg aggggctcgg gccgacgggc
      301 tgggccaacc tgtgcgacca cgacggcgac gacgcggtcc cgctcgccgg gaagtggcag
      361 cggtcagggt tccacgagac cgccccggac cggatcagct tcgcgtgcac cggcgcggtc
      421 gcctacaagt gcctggtgtg gggttacctg gcgggcagtg acagcacgtc gctcggctgg
      481 cgggcgcacc aggcgtgcac gcggatggcg cgcggcgact actgcgccaa cggccactcg
      541 cacacccgcg agggcacgac catcacgatc tacgacttcg ccggcgtcac ctcgccgccg
      601 ccgctgcact tcgacggcgt ggtggactgg gcgccgaacc ccaaccggat gttcttcgag
      661 gcggcgtgga gcgatggcgc gcacccggcg agctgcctga gccggctgcg ctggcagagc
      721 ctgccgctcg gcccgctgtg cgacgacggc gatctgcgcg atccccggct cgacaccggc
      781 gtgcggttct gcgaggacat cgcgtggccg cccccgggca gcgagccgac cggcgcgctg
      841 ctgttcaacg cctcgcgcta caccgacatg gcgctccacg tctggcagcg cggcgacgac
      901 atggtgagca ccgtccaggg gttctaccag ctgccgaacg tgatccagcc gttccccagc
      961 gtcgggccct acgagcacat ccgcaacgac ggcagcgtgc tgcgctcgct gcgcagcgac
     1021 caggaccggg cgagctacga ggatctgtgg ctgtaccgca atggcagcga cttcgtggtc
     1081 gcgggcgcgt cacggccacc cccgggcttc accccgggct cgttcgaggg cttcgccgcc
     1141 aagcaacaga gccccgggat gttgccgttc aacctgtaca tccaccagga caccggggac
     1201 accctgtcga cgaccgggac accggatgca gcctacgtgc tgcagtggac gatcggctac
     1261 gtggcgccgc cggagacgcg ctgagatcga tcgagagggg gctttcccgc cccctctttc
     1321 gcggcgaagc cgcgaaattc acccctccgc gcgtcgcgtc gcgcggccct gcacctgagc
     1381 gctctgtgct cgtcgatcga gcgggcgcgt cgcgtcgcgt cgccctgcac ctgggctctg
     1441 tggtcgtcga tcgtccagcg tcacctgcac acctgcgaga tcaccgcggg gtcgcgcaga
     1501 tccgcagccc cgaccacagc cagcggatgc ggccgtcgtt gaagtcgcga ttggcgatct
     1561 cgccggacgc cgcgccctgg taccagctgc cgccgcgcag catggcggtg ccggacgggc
     1621 cgggctcgac ccactcccac atgttgccga
//
LOCUS       DASVOR010002362         6705 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_404867,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002362 DASVOR010000000
VERSION     DASVOR010002362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6705
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404867"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VF516_43515"
     CDS             <1..273
                     /locus_tag="VF516_43515"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8114700.1"
                     /translation="KFTPNGGRVTVSLEWLGARAVIRVRDTGIGIEPSQIPALFEPFV
                     QAAQGLDRSRGGLGIGLAVARSIVELHGGSIAMHSEGPGAWHRSHV"
     gene            215..709
                     /locus_tag="VF516_43520"
     CDS             215..709
                     /locus_tag="VF516_43520"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8114701.1"
                     /translation="MAASRCTARGLGRGTAVTFDLPIESPNRFAERPSAGAAPVVKRR
                     VLIIEDQPDAATTLQSLLALQGHEVRTAPDGAAGLALARAFDPDVVLCDLGLPIMDGF
                     AVARAIRADPALRDRRVVAVTGYAQPEDIARSRSAGFDSHLAKPLTLASVEAELALAN
                     RSAV"
     gene            901..2094
                     /locus_tag="VF516_43525"
     CDS             901..2094
                     /locus_tag="VF516_43525"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8114702.1"
                     /translation="MRRAIWLLGAGQCVYWGMLYYGFSVLLVPMVDELHSTRAVIAGA
                     FSVGLLVMALVAPQIGRWVDRDHAPRVMRIGAWIAVGGALAASQVPGVVSLYVVWAIL
                     GLAMASLFYEPALGLVIRTVRIDSERLRALASVTVVAGLASTTSLPVMAFTVKRIGWR
                     STEIAIAAVVVVASASLQRYVLPSFQVESAHAPAAAAGAPSVPVRRPAAFAVLAVVFV
                     LSMLGSMALTTILIPVVVERGQSPTTAATVLAALGVMQLPGRIWMLRGGRARSIRGLL
                     ALQLGLQTAGMTLVAVNGWIGLTAIGVACFGGGSGLHTLARPWALQSIYGVSEAGRVN
                     GVMARYEGFARASGPVLVALLYDQAGAVTVFGGLGAALLMVAPISWAVLTASPTSTDG
                     VTSRS"
     gene            complement(2078..2506)
                     /gene="rhuM"
                     /locus_tag="VF516_43530"
     CDS             complement(2078..2506)
                     /gene="rhuM"
                     /locus_tag="VF516_43530"
                     /inference="COORDINATES: protein motif:HMM:NF024706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
                     /protein_id="HEX8114703.1"
                     /translation="MTQAPEPTGQILLYRSEDGRTKLDVRLENETVWLSQRQLTEPFG
                     KAKGTLSEHIKHIFEDGELDPAATVRPFRTVQREGDREVSRDVDFYNLDMVIALGFRV
                     RSPPAVRFRSGRPTGSRGVAVISRPLRAVRVPRAVASSGW"
     gene            2884..4008
                     /locus_tag="VF516_43535"
     CDS             2884..4008
                     /locus_tag="VF516_43535"
                     /inference="COORDINATES: protein motif:HMM:NF024810.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kelch repeat-containing protein"
                     /protein_id="HEX8114704.1"
                     /translation="MENQRSCATTGMSFSRIMLLNVVLSLFAGCGTWTPTPPMSDQRY
                     RHAATRLKDGRVLITGGANTGFALLTSADIYDPATNAWTTTIMNSWRFQHTATLLTNG
                     KVLVVGGGHNAVSLSSAELFDPIAKTWTAVAPMATERAEHTATLLGNGMVLVAGGWDG
                     SKYVSSAELYDPTTNTWVATGSMSFNHGGHTATLLGNGKVLVTGGLDGLGHVLRNAEL
                     YDPATNTWSSSTLMAKARAYHTATLLGSGKVLVTGGSNTIGGGVMLSSTEVFDPNGNL
                     GNGSWTSVGDMHDPRDTHTATLLVDGTVLVTGGWTGTAVVASVETYSSAVEAWSIEKP
                     MQDTRAEHAATLLDNGNVLVTGGLSQPGGAWSSAEVYAAP"
     gene            complement(4163..4768)
                     /locus_tag="VF516_43540"
     CDS             complement(4163..4768)
                     /locus_tag="VF516_43540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114705.1"
                     /translation="MADQGILAALARRGEHPRRPATLAMVVCSVILVFAGGCFMSARK
                     GPTGLLGLHWGDDATEGARRLGLTADRWDPWIDPAFETSIDFDHPRPALGVEGLVRLI
                     RVVADKQLEGVQVIYRGCAGDDAQKQLLRKGLHSELHVKSVDVDVPYEIWRDNSLVHF
                     VADPSDGTCTLTVAGPRFGKAFEAALLRGGLGNVGGAVGPK"
     gene            complement(4771..>6705)
                     /locus_tag="VF516_43545"
     CDS             complement(4771..>6705)
                     /locus_tag="VF516_43545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114706.1"
                     /translation="AHEAAHVVQQRGGVQLKGGVGESGDAHEQHANAVADAVVQGKSA
                     EPLLDRYAGGSTSGSATSGGHPIQCFGSQEHQSLGDTATGTAHYDVGGGANDQFQLTH
                     GDIVALNGDYFLAGPGTTSAGGRGQQDDLFYLAGRPGNRGQAPGTRDEVIWTLKMIRG
                     NDARFAAGGPWASYVFSDTVKAAVNERYQRLAAANTTHFAAPRGRDASGQPNASPEGS
                     AGNSYRSTHEAALRMAFQAGQRHDPIDRAMAMEAAAQHYLTDSFSAGHVRTPIGDLRE
                     YWGTKYPLFWYNLRHKMALDTAVRLNAQSNNITTALGTVNQMYNAISQQIETMASSLP
                     AVTLGDLLAKVFHDRDNAEGVDIQGGGRIWGDDNLDNPDPRNQTRALAQAAIRDGNAD
                     VQAGYRLGGQAASGSTTPDDQIFAQVRSATATGNRYRAETRMPAPASTEAPQNWRAGS
                     LDELWARPVCGTSGPAVGAKIIEALQPGHEIRQTLEDLANRFPETDSHFTGDLQPRRA
                     YRDGFLAPLVADPHAGLNHIINWAPNYGLASNSRDDQSLASGQELDRAHQLGGMTTAA
                     RVAYINELLDGYTAEDEGALVVRVFETAPAGERRAMYRQIEGHDWTGDFKHGMFVSDD
                     KLWNSLTSGQLNQLRGIING"
BASE COUNT          950 a         2192 c         2409 g         1154 t
ORIGIN      
        1 aagttcacac cgaatggcgg gcgggtgacg gtgtcgctgg agtggctcgg tgcgcgggca
       61 gtgatccgcg tgcgggacac tggcatcggc atcgagccgt cgcagatccc tgcgctgttc
      121 gagccgttcg tccaggcggc ccagggactc gaccgctcgc gcggcggtct cggcatcggg
      181 ctcgccgtgg cgcgcagcat cgtggagctg cacggtggca gcatcgcgat gcacagcgag
      241 gggcctgggg cgtggcaccg cagtcacgtt tgatctgccc atcgaatcgc cgaaccggtt
      301 cgctgagcgg ccatctgccg gcgcggcgcc cgtggtgaag cgccgggtcc tgatcatcga
      361 ggaccagccc gatgcggcca ccaccttgca gagcttgctg gcgctccagg gccatgaggt
      421 gcggaccgcg cccgatggcg ccgccggact cgcgctcgcc agggcgttcg atcccgacgt
      481 cgtgctgtgc gacctcgggt tgcccatcat ggacggcttc gcggtggcgc gcgcgatccg
      541 tgctgatccc gcgctccgcg accgtcgcgt cgtcgcggtc accggctacg cgcagccgga
      601 agacatcgcc cggtcgcgca gcgctggctt cgacagccac ctcgccaagc ccttgacgct
      661 cgccagcgtg gaggcagagc tggccttggc caatcgctcc gcggtctagg atgattccag
      721 gtcgctgccg agcgcagtct gccgctggga tctgaccatg accgccgcat ccaagagcat
      781 cctgtttctt tgcgtcgcaa attcggcccg cagcgcgccg ccggccggtt gcgatgactt
      841 cgtcgcagcg ttgcgcggtg acggcgccgc cccgagagcg ggcgcccggt gacgccgtcg
      901 ttgcgacgcg cgatctggct gctcggcgcg gggcagtgcg tctactgggg aatgctgtac
      961 tacggcttct cggtgctgct cgtgccgatg gtggacgagc tccactcgac gcgcgccgtc
     1021 atcgccggtg cgttctcggt cggcctgctc gtgatggcgc tggtcgcgcc gcagatcggg
     1081 cgatgggtgg accgcgatca cgcgccgcgc gtgatgcgga tcggcgcctg gatcgctgtc
     1141 ggcggtgcgc tcgccgcatc gcaggtcccc ggcgtggtca gcctgtacgt ggtctgggcg
     1201 atcctcgggc tcgcgatggc ctcgctgttc tacgagcccg cgctcggcct ggtcatccgc
     1261 acggtgcgga tcgacagcga gcggctgcgc gcgctcgcgt cggtcacggt cgtcgccggc
     1321 ctggccagca cgacctccct gccggtcatg gcgttcaccg tgaagcgcat cggatggcgg
     1381 tcgaccgaga tcgcgatcgc ggcggtggtg gtcgtggcga gcgcgagcct gcagcgctat
     1441 gtgctgccga gcttccaggt cgagtccgcg cacgcccccg ccgcggcggc cggcgcgcca
     1501 tctgtccccg tccgccggcc cgcggcattc gcagtgctgg cggtcgtgtt cgtgctcagc
     1561 atgctcggca gcatggcgct gacgacgatc ctcatcccgg tggttgtcga gcgcggccag
     1621 tcgccgacga ccgcagcgac ggtgctcgcc gccctgggcg tgatgcagct gccgggaagg
     1681 atctggatgc tgcgcggtgg gcgggcgaga tcgatccggg gcctcttggc gctgcagctc
     1741 gggctgcaga ccgccggcat gaccctggtc gcggtgaacg gctggatcgg cctgaccgcg
     1801 attggcgtcg cgtgcttcgg cggaggttcg ggcttgcaca cgctggcccg gccgtgggcg
     1861 ttgcagtcca tctacggcgt ctccgaggcg ggacgcgtca acggcgtgat ggcgaggtat
     1921 gagggcttcg cgcgcgccag cggcccggtc ctcgtcgcgc tgctctatga tcaggccggc
     1981 gcggtgaccg tgttcggtgg gctcggcgcc gccctgctga tggtggcgcc gatcagctgg
     2041 gcggtgctca ctgcgagtcc gacgagcaca gacggtgtca ccagccggag ctagccaccg
     2101 cgcggggtac acgtactgcc cgcagggggc gtgagatcac ggccacgccc cgtgagcctg
     2161 tcggccgccc actgcggaag cgaacggcgg gcgggctccg gacccggaac ccgagggcga
     2221 tgaccatgtc gaggttgtag aagtcgacgt ctcgggacac ctcgcggtct ccctcccgtt
     2281 gaactgttcg gaatggccga acagttgccg ccgggtcgag ctcgccgtcc tcgaagatgt
     2341 gcttgatgtg ctcgctgagg gtccccttgg ccttgccgaa gggctcggtg agctgacgct
     2401 ggctgagcca gaccgtctcg ttctcgaggc ggacgtcgag cttcgtgcga ccgtcttcgc
     2461 ttcggtagag caggatctga ccggtcggtt cgggcgcttg ggtcatcagc atcctcaccg
     2521 cgctcccggc catttcagga agccaagcta tcctggttac ttagagcgat cgaccatcgt
     2581 acggtgttgg cgggatggca tctgctgcgc caaggcgtcc gtccccgtcg atggcacatg
     2641 tgccaggcga agtggcacgt gccagacctc gatgggcagg tacgacgtct cgcgcaggac
     2701 cgattcagac cagcctcccc cgcccatcgg aactctgcca tgtccggcga tgttgacgag
     2761 atcgttcaag gaagtggagc gcgccgcggt ggacgaacct ggcgtcatct aatgcccgac
     2821 tgcataatgc ctgcagcggc gagtcgacgt gcgtgggtac aggccctgca ctgcggcgaa
     2881 gtcatggaga atcagcgcag ctgtgcgacg acgggaatgt cgttttcgcg aatcatgctc
     2941 ttgaacgtgg tgctgtcctt gttcgcgggg tgcggtacgt ggaccccgac gccgcccatg
     3001 agcgaccaac gatatcgcca cgcggccact cgattgaaag atggaagggt cctgatcacc
     3061 ggaggggcga atacgggctt cgcgttattg accagcgcgg acatctacga tccggcaacc
     3121 aacgcctgga ccacgacgat aatgaattcg tggcgcttcc agcacaccgc aactctgctg
     3181 accaacggca aggtgctggt cgtgggtggc ggccacaatg cggtgagtct ttcgagcgcc
     3241 gagctgttcg acccgattgc taaaacgtgg actgccgtgg ccccgatggc cacggagcgt
     3301 gccgagcaca ccgcgacgct gcttggcaat ggcatggtcc tggtggcggg cggttgggac
     3361 ggctcgaagt acgtatcgag tgcagaactg tatgacccca ccaccaatac gtgggtagct
     3421 actggatcga tgtcattcaa tcacggtggg catacagcga cgctgctcgg caacggtaag
     3481 gtcctggtca cgggcggtct ggacggcctg gggcatgtcc tgaggaacgc cgaactgtac
     3541 gacccggcga ccaacacctg gagctcaagc acgctgatgg ccaaggcacg cgcgtaccac
     3601 acggcgacgc tgctcggcag cgggaaggtt ctggtgaccg gtggcagcaa taccatcggc
     3661 gggggagtga tgttgtcgag tacagaagtc tttgacccca acggcaacct gggcaatggc
     3721 tcgtggacat cggtcggaga tatgcatgac ccgcgcgaca ctcacaccgc gacgctcctc
     3781 gtcgatggca cggtgcttgt aacaggcgga tggaccggca cggcggtcgt ggcgagcgtg
     3841 gaaacgtaca gttcggcggt cgaggcctgg agcatcgaaa agccaatgca ggacacgcgt
     3901 gctgagcacg ctgcgacgct gctcgataat ggcaacgtgc tagtgaccgg cggcctcagc
     3961 cagccgggcg gcgcgtggtc cagtgccgaa gtctacgccg cgccgtgaac gtcagcgtga
     4021 gcacgatgtt tgatatggca acggccacga tgtttgggta tgcgcggcga gcacgatggg
     4081 cagtggcccg cggggcagcg accgtgacgc gctacttcat gacgggtccg ctcgcgtgcg
     4141 tgtccaccgg ggggcctggt ggctacttcg gccccacggc tccgccgacg ttccccaggc
     4201 ctccacgtag aagcgcggcc tcgaaggcct tgccgaaacg gggcccggcc acggtgagcg
     4261 tgcaggtgcc gtcgcttgga tcggcgacga agtggacgag cgagttgtcg cgccagatct
     4321 cgtacggcac gtcgacatcc accgacttga catgcagctc gctgtggagc cctttgcgca
     4381 gcagctgctt ctgcgcatcg tcgccggcgc agccgcggta gatcacctgg acaccctcga
     4441 gctgcttgtc ggcaacgacg cggatgaggc gtaccagtcc ctcgacgccg agcgccgggc
     4501 gcgggtggtc gaagtcgatg ctggtctcga acgcgggatc gatccacgga tcccagcggt
     4561 cggcggtcag ccccaggcgg cgcgcgcctt cggtggcatc gtctccccag tggagcccga
     4621 ggaggcccgt gggccccttg cgtgcggaca tgaagcaacc tcccgcgaag acgaggatca
     4681 cggagcaaac gaccatggcc agcgtcgcgg ggcggcgtgg gtgctccccg cgacgggcga
     4741 gcgcggcaag gatgccttgg tctgccatgg ctacccgttg atgatgccac gcagctggtt
     4801 gagctgcccg ctggtcagcg agttccacag cttgtcgtcg gaaacgaaca tgccgtgctt
     4861 gaagtcgccg gtccagtcgt ggccctcgat ctgccggtac atggcacgcc gctcgccggc
     4921 cggtgcggtc tcgaagaccc ggaccaccag cgcgccctcg tcctcggccg tgtagccgtc
     4981 gagcaactcg ttgatgtagg cgacgcgcgc cgccgtggtc atcccaccga gctgatgggc
     5041 gcgatcgagc tcctggccgc tggccagcga ctgatcgtcg cggctgttgc tcgccaggcc
     5101 gtagttcggc gcccagttga tgatgtggtt caggccggcg tgggggtcgg cgaccagcgg
     5161 cgcgaggaag ccgtcccggt aggcacggcg tggctggaga tcgccggtga agtggctgtc
     5221 ggtctccggg aaccggttgg cgagatcttc gagcgtctgg cggatctcgt ggccgggctg
     5281 gagggcttcg atgatcttgg cgccgacggc cggcccgctc gtcccgcaga ccggccgagc
     5341 ccacagctcg tcgagcgagc cggcacgcca gttctggggt gcttcggtcg aggccggcgc
     5401 cggcatgcgc gtctcggcgc ggtagcggtt gccggtggcg gtggccgaac gcacctgcgc
     5461 gaagatctgg tcgtcgggcg tcgtcgagcc gctggccgcc tggccgccga gccggtagcc
     5521 ggcctgcacg tcggcgttgc cgtcgcggat cgcagcctgc gcgagcgcgc gcgtctggtt
     5581 gcgcggatcg gggttgtcga ggttgtcgtc gccccagatc cggccgccgc cctggatgtc
     5641 gacgccctcc gcgttgtcgc gatcgtggaa caccttggcg agcaggtcgc ccagcgtcac
     5701 ggccggcagg ctcgaggcca tcgtctcgat ctgctgcgag atcgcgttgt acatctggtt
     5761 gaccgtcccg agcgccgtcg tgatgttgtt cgactgcgcg ttgagccgga ccgcggtgtc
     5821 cagcgccatc ttgtggcgca ggttgtacca gaacagcgga tacttcgtgc cccagtactc
     5881 tcggaggtcg ccgatcggcg tgcgcacgtg gccggcggag aacgagtccg tcaggtagtg
     5941 ctgcgccgcg gcctccatcg ccatcgcgcg atcgatcggg tcgtggcgct ggcccgcctg
     6001 gaacgccatg cgcagcgcgg cctcgtgcgt gctgcggtac gagttgccgg cgctgccctc
     6061 gggcgacgcg ttgggctggc cggacgcatc gcgcccgcgc ggcgcggcga agtgcgtcgt
     6121 gttcgccgcg gccaggcgct ggtagcgctc gttgaccgca gccttgacgg tgtccgagaa
     6181 cacgtagctg gcccacggtc cgcccgccgc gaagcgcgcg tcgttgccgc ggatcatttt
     6241 cagcgtccag atcacctcgt cgcgggtacc aggcgcctgg ccgcggttcc cggggcgccc
     6301 cgcgagatag aacaggtcat cctgctggcc tcggccgccg gccgaggtgg tgcctgggcc
     6361 cgcgaggaag tagtcgccgt tcagcgccac gatgtcgccg tgggtgagct ggaactggtc
     6421 gttggccccg ccgccgacgt cgtagtgtgc ggtccctgtc gccgtgtcgc cgagcgactg
     6481 gtgctcctgc gacccgaagc actggatggg atggccgccc gaggtggccg agccgctcgt
     6541 cgagccgccg gcgtagcggt cgagcagggg ctcggcgctc ttgccctgca cgacggcgtc
     6601 ggccaccgcg ttcgcgtgtt gctcgtgcgc gtcgccggac tcgccgacgc cgcccttgag
     6661 ctgcacgccg ccgcgctgct ggacgacgtg agctgcctcg tgcgc
//
LOCUS       DASVOR010002363         4308 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_434329,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002363 DASVOR010000000
VERSION     DASVOR010002363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4308
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434329"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..165
                     /locus_tag="VF516_43550"
     tRNA            84..165
                     /locus_tag="VF516_43550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:118..120,aa:Leu,seq:cag)
     gene            complement(233..1660)
                     /locus_tag="VF516_43555"
     CDS             complement(233..1660)
                     /locus_tag="VF516_43555"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX8114707.1"
                     /translation="MIRSRSVTVEEFPEALDNGVAASRRRGMIPSGKSSLRDGEHSMR
                     QSGQKVHGPYQHGNKWRLLVRDADGGQEAVSFPTEAAANAHKRSLLKQIAGRTVSSAV
                     QEFVVEKREIGLRSGTVKRAEFHLRAFFQLDGEVDGVSMPYANTGGMVEDLKPQTCEQ
                     LYRAMVKTCAVDTHRNALAEVKAFAAWCVKQGWIATSPLADVKPIGQRNRGKKQLRID
                     EARKLVDLCIREANAGDEGAVGAMTAFLLGLRASEVTDRVVRDLDDDGRQLWIEFGKT
                     KRSRRTLEVPALLRPYLLALAKGRAPDAQLISRTVSRRSGKKRDRYWLLYHVERLCAE
                     AEVPVVCTQSLRGLHASVATDAGATSHVVASALGHSSPAVTHAHYIDGATARRARTRR
                     VIGKVAPDPALRVVAGTTPEAAGAASQAPTGAPKKARKPARRASSHVGPDPGPRVVGG
                     EASGAAAAAAEPTGSPQLVVTGNGR"
     gene            complement(1657..3177)
                     /locus_tag="VF516_43560"
     CDS             complement(1657..3177)
                     /locus_tag="VF516_43560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114708.1"
                     /translation="MKQLTVTTGRSDDDDENDDSVTDDRGDGDGGDGGDGGDGGDDDN
                     GGASGDGTPSTARHWSEAWLPVVNHVLMQVMPMVNVWAENQQAEARARRADATMKEVA
                     DVSGAHASVDEPAAVQAEHARAEQLAAAVAAASSPGRGVDVMKLLQLLPPATSAKLMK
                     IQMALSPDEQADAMKILRGFPVEEMQDLLTTFDIASLDECTDFLRRLIHDWRKTQAAR
                     RARATEPRPADHVGDGEAIENAVDPRAAYAASREALRAAQAEQERAAQEHAAQLASAI
                     ATASSPGPGINVFSLLRLLPPYTASKLVHINAALSPEEQADAMQVLKSYSVEAMHELL
                     TAFDANPVEECTAFLRRVIADWRAHQAAELARAKEAPPGGSGSGGMSGAAGPISAAGS
                     SGTSGASGLSSAAGSSSPSRAADPSGAGGKTSSGVAVSSIGASGAGGSGSPSRAADPS
                     GATGPSETSHAAGSSDAAGSSSAEGPSSAEGSSSAIAPSHTSGAPGRGGSGSKKPR"
     gene            complement(3174..3782)
                     /locus_tag="VF516_43565"
     CDS             complement(3174..3782)
                     /locus_tag="VF516_43565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114709.1"
                     /translation="MEKMSPHPPRSPSTVPLARDTDGNLIELPDGAVGWRIRRQTGGR
                     PRLLLDVRKQPMLFPLDYTMADVEDVLSPGSYLLDVVDENGEPLGVTVAVSTGMLRNA
                     ESIDPDNSNSNDSAATMPTVVPTSLPNTTSDVRLVLEANVRATQMAFIHNQRTLEIGL
                     RMAETLRDSVQLLASSQADWIKSISSARGFFRNAGQPLTRSK"
     gene            complement(4048..>4308)
                     /locus_tag="VF516_43570"
     CDS             complement(4048..>4308)
                     /locus_tag="VF516_43570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114710.1"
                     /translation="TSLKMALGCDTGVPPVFCGLLLSVPSKGREAMLAEGGLRHAFRG
                     PSLGSWRLDRREEAFPEGFQQGLTKGIHQSFPPLRAGIQAKQ"
BASE COUNT          631 a         1504 c         1374 g          799 t
ORIGIN      
        1 gatcagtctt gcacctggac cttgtgctcg gcgccgagct gtggcatccc taccttcctt
       61 cgagccaccc ggctcggggc cctgcgaatg tggcggaatt ggtagacgcg ctggtttcag
      121 gtaccagtgg gtaacaccgt gtcggttcga gtccgaccat tcgcacgaag aatttcgccc
      181 ctttgcgtcc cctggatgaa agggacgaaa gtcttttcgg ctttctggta actcatcggc
      241 cgttaccagt taccaccaac tgcggcgaac cagtgggttc ggccgcagca gccgctgcac
      301 cgcttgcctc gccgccgacc acccgcgggc cgggatcagg ccccacgtgg ctgctcgcgc
      361 gcctggccgg tttccgagcc ttcttcgggg ctcccgtggg tgcctgggag gcggcccccg
      421 cggcctcggg ggtggtcccg gcgaccaccc gcagcgccgg atccggagcc accttgccga
      481 tcacgcgccg ggtgcgcgcg cgccgggccg tggcgccatc aatatagtgc gcgtgcgtga
      541 ccgctggcga gctgtggccg agcgctgagg cgaccacgtg cgaggtcgct ccggcatcgg
      601 tggcaaccga ggcatgcagc ccccgcagcg actgcgtgca gacgaccggc acctcggcct
      661 ccgcgcacag ccgttcgaca tggtacagca gccagtagcg atcccgcttc ttgccgctgc
      721 gccgggagac cgtccggctg atcagctgcg catccggggc gcgacccttc gcgagcgcaa
      781 gcaggtaggg gcgcaggagt gccggcacct cgagcgtgcg ccgcgaacgc ttcgtcttgc
      841 cgaactcgat ccagagttgc cggccgtcgt catccaggtc gcgcaccacg cgatcggtga
      901 cctcggacgc gcgtagcccc aggaggaacg cggtcatggc cccgaccgcg ccctcgtcgc
      961 ccgcgttcgc ctcgcggatg cacaggtcga cgagcttgcg cgcctcgtcg atccgcagct
     1021 gcttcttgcc ccggttgcgc tgcccgatcg gcttcacgtc tgcgagcggg ctggtcgcga
     1081 tccatccctg cttcacgcac cacgcagcga acgccttgac ctccgccagc gcgttgcgat
     1141 gcgtgtcgac ggcgcaggtc ttcaccatcg ccctgtagag ctgctcgcag gtctgcggct
     1201 tcaggtcctc gaccatcccg ccggtgtttg cgtacggcat gctcacaccg tccacttcgc
     1261 cgtcgagctg gaagaacgcg cgcagatgaa attctgcgcg tttcaccgtg cccgagcgca
     1321 agccgatctc acgcttctcg acgacgaact cctgcaccgc gctgctcacc gtgcgtccgg
     1381 cgatctgctt gaggaggctc cgcttgtggg cgttcgcggc tgcctccgtt ggaaacgaga
     1441 ccgcctcctg gccaccgtcc gcatcccgga caagcagccg ccacttgttg ccatgttgat
     1501 agggtccatg aaccttctgt cctgactgtc gcatcgaatg ttctccatcc cgaagcgacg
     1561 atttcccgct cgggatcatg ccacgccgtc tcgacgctgc aacgccgttg tcgagggctt
     1621 ctggaaattc ttcgacggtg accgagcgag atctgatcat cgcggcttct tcgatccgct
     1681 gccgccccga ccgggcgcac cgctcgtgtg gctcggagcg atcgcagagc tcgaaccttc
     1741 cgcagagctc ggaccttccg cagagctcga accggctgcg tcgctcgaac cggctgcatg
     1801 gctcgtctcg ctcggccccg tcgcgccgct cggatcagct gcacggctcg ggctccccga
     1861 accgcccgca ccactcgcgc cgatcgagct cacggcaacg ccggagctgg tcttaccgcc
     1921 cgcaccgctc ggatcggctg cacgactcgg gctgctggaa ccggccgcgc tgctcaaacc
     1981 ggacgcgccg ctcgtcccgc tcgaaccggc cgcgctgatg ggaccagccg caccgctcat
     2041 cccgccgctg ccgctgccgc cgggcggtgc ctccttcgcg cgcgccagct ccgccgcctg
     2101 atgtgccctc cagtccgcga tcacgcggcg caggaaggcc gtgcactcct cgacgggatt
     2161 tgcgtcgaac gcggtgagta gctcgtgcat cgcttcgacg ctgtagctct tgagaacctg
     2221 catcgcgtcc gcttgctcct cgggtgacag cgctgcgttg atgtgcacga gcttcgatgc
     2281 tgtgtaggga gggagtagcc gcagcaggct gaacacgttg atgcccggac cgggagacga
     2341 cgcggtggcg atggctgatg ccagctgcgc agcgtgctcc tgtgccgctc gctcctgctc
     2401 cgcttgcgcg gctcgcagcg cctcgcgcga ggccgcgtac gcggcccgcg gatcaactgc
     2461 attttcgatc gcctccccgt cgccgacgtg gtctgccggt cgtggttccg tggcgcgtgc
     2521 gcgcctcgct gcctgcgtct tgcgccagtc gtggatgagg cgccgcagga agtcggtgca
     2581 ttcgtcgaga gacgcgatgt cgaaggtggt gaggaggtct tgcatttcct cgacggggaa
     2641 gcccctgagg atcttcatcg cgtccgcctg ctcgtcgggc gacagcgcca tctggatctt
     2701 catcagcttc gcggaggtgg cgggcggcag caactgcaac agcttcatga cgtcgacgcc
     2761 gcgacctggt gatgatgctg cagccacggc cgctgccagc tgttcggcgc gggcatgttc
     2821 cgcttgcact gcagcgggct cgtcgacgct cgcgtgcgcg cccgatacat cggccacttc
     2881 cttcatcgtt gcatcggctc ggcgagctct cgcctccgcc tgttggttct ccgcccacac
     2941 gttaaccatc ggcatcacct gcatcaagac atggttgacc accggcagcc acgcttccga
     3001 ccagtgccgc gccgtcgacg gtgtgccgtc gccactggcc ccgccgttgt catcatcgcc
     3061 gccatcgccg ccatcgccgc catcgccgcc atcgccgtcg ccgcgatcat cggtcacgga
     3121 atcgtcgttc tcgtcgtcat catcgctgcg gcccgtcgtc accgtgagct gcttcacttc
     3181 gaccgggtga gcggttgccc agcgttgcgg aagaacccgc gcgccgatga gatggacttg
     3241 atccagtcgg cctgcgagct ggcaaggagc tgcacgctgt cgcgcagggt ctcggccatg
     3301 cgcagcccga tctcgagcgt gcgctggttg tggatgaacg ccatctgtgt cgcacgcacg
     3361 ttcgcctcga gcacgagccg cacgtcgctg gtggtgttcg ggagcgatgt cggcaccacg
     3421 gtcggcatgg tcgcggcgct gtcgttgctg ttgctgttgt ccgggtcgat cgattccgcg
     3481 ttgcgcagca tcccggtcga aactgcgacc gtcacgccga gcggctcgcc gttctcgtcg
     3541 accacgtcaa gcaggtagct accgggagag agcacgtctt cgacgtccgc catcgtgtag
     3601 tcgagcggga acagcatcgg ctgcttgcgg acgtccagca gcagccgggg tcgccccccg
     3661 gtttggcgac gaatgcgcca cccgacggca ccatcgggga gctcgatcag gttgccgtcg
     3721 gtgtcgcgcg cgagaggaac ggtcgacgga ctacgtgggg ggtgagggga catcttctcc
     3781 atggggacct ctggccgatg cgcacgacgc tgttgccgtc gagcatgcgc gcgtgatgcc
     3841 gcgtcctacc gcgcggcgag aactccgatt cccggcaggc aaccgaagtg accgggcatg
     3901 gactcgatcg tcgagagcca tcagctctcc gcagcccggc tggtaagcgg ttgatctctc
     3961 gacccgcctc gccgggaagc ctttcgcagc ggctttgcag accttcgaga agcttttcca
     4021 aagagctgca gggtggtcga tgaaccgtca ttgtttcgcc tggatgccgg cgcgcagcgg
     4081 gggaaagctt tgatggatgc ctttggtaag accttgttga aagccttccg gaaaggcttc
     4141 ttcccggcga tccagcctcc atgaacccaa cgatggccca cggaacgcgt gccggaggcc
     4201 cccttccgcc aacatcgcct cacgtccctt ggatggaaca gatagaagca ggccgcagaa
     4261 cactggcgga acacctgtgt cgcaccctaa tgccattttc aagctggt
//
LOCUS       DASVOR010002364         5143 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_376623,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002364 DASVOR010000000
VERSION     DASVOR010002364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5143
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376623"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VF516_43575"
     CDS             <1..295
                     /locus_tag="VF516_43575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114711.1"
                     /translation="LRGVGSGRVLRVTGFDRVQELVGHLIVEANLPQPGAPRRDDYDG
                     DGYIIVRHPETEVVHAAVKTITETIGVEYADEADPPRPAAPSASGSAKRVDDH"
     gene            621..1721
                     /locus_tag="VF516_43580"
     CDS             621..1721
                     /locus_tag="VF516_43580"
                     /inference="COORDINATES: protein motif:HMM:NF019040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEX8114712.1"
                     /translation="MLLGGGIDAILSMDPKTKSEVDPAVDLPYDARGIHSKGELAFGP
                     NMAPLAPWAEHAAVLRGVLTSSVAHIPAIGQLVLMRRGIPPGGKHVAFLDLVGGAHRT
                     AQLPVVRFGNAGPVAPKGLITSDTVFERLLTLSPEELDVVRQQLRTQADWLAGSRDRE
                     GAATASRLRGTVEFLDRLSALPGPAAEAWTTAPEAQEFAEQLQRCLWLFEHDLTACAL
                     INHQRWDTHIDNFAEQGRLSSALMPVLARFVEELAKRTNSHGSMLDQVRVIATSELGR
                     FPKLNTERGKHHFPEVPMLFIGAGIPGGHAFGRTGRMMEALPMDLNTGRATGNGGSAV
                     TLDDVGATLLRSVGVDPELYGNSGRHLPFLVP"
     gene            1721..3796
                     /locus_tag="VF516_43585"
     CDS             1721..3796
                     /locus_tag="VF516_43585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114713.1"
                     /translation="MRRRLVAVVCFLAACQTHAPRATRPDVHADVGPGGGATPAGGGA
                     TPAGGGATLKVDPRRANEIAADLFLLASNRVPSRQELAEASGALSAGQQELRAFVAGL
                     VATDASTAFASFALFPWESNPGNPPQGFGQLFFELGTFEQAGQTVHFLTGGNVVGDSV
                     VAGSAKPPCTLREAERVRPWWDPEHEILVCPSSHRPDRIRAINGKHKLCSPVTPEFER
                     QGCGCGPSLMYCIKSERLDDISLSLHKEFNDTIAWAVGQDRPLQDVFRGRETVRDWLV
                     EWLYRRQLVESGAPAERLSAAGWAPGRSPRPELHPGAHAGLLTSPQVALLGCEPRERL
                     MQISNLIWCVKPRSVSVTADTVFKLPTNFRAPMQGWQLLASMPVCDGCHARMDYGAQF
                     FAGMTAGFHYDSQISPHAGSTMSMFMNDQFDLRGKGEQTPAGFAELAVSQPEFGACMS
                     QRVAAHVFGQHATADDVGSLLEVFKTRPTYRALLTSALVSYAERRLWSAGERGEIAHA
                     DPAAAQLGLGALVEQYCSDCHDAGSPAEKVWNSTRALADHGAMVQALDSIGDRRMPPA
                     RDPLDPAVRQRFVALLAERIWPDTEPRSAALDNYSGRLDNVEVHRRASTMKIIGALAG
                     VDLDDAIAKLAAFYAGRGRDDQEYTPDFALNTVIDGVAACKRAGAKDVIDCVKRTTRL
                     DAILPVAPR"
     gene            3836..5008
                     /locus_tag="VF516_43590"
     CDS             3836..5008
                     /locus_tag="VF516_43590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114714.1"
                     /translation="MTTDTHHREFGAFLPWRSFDLPVVPVSHPWGDDCVLFGSGVDAF
                     RGLLEHGRSTRGWQRLWAPSYFCQEVMRSLLTAGLQVLLYDYGPEDTFPLGARISAKP
                     GDVVLRMNFFGLHDRLSTDGIDRNRVEILDDHTHDPWSDWAYTSDADWCVVSPRKTLP
                     LPGGGVLWSPRRHPLPTPSPVTDTRRAATFEKFTAMVLKGLYLQGYPLDREIYWRLND
                     AAEDHMASGEASGVPEWIGDLLKCFPVREWREYRRNNCLAVSNVLKGLPWLKVLEADG
                     GGGICQFSGLVLFDSEERRNFVRRQLADRRIFLTVLWELDDAPIPEIPEEHRRFSKTL
                     LCIPCDARYDRVAVEKVSGLIRDIGNGFASSGRAALNNASIEHQQPLARPAGASSP"
BASE COUNT          846 a         1729 c         1689 g          879 t
ORIGIN      
        1 cctgcgcggg gtcggcagcg gtcgggtgct gcgggtcacc ggcttcgatc gagtccagga
       61 gctggtcggc catctcatcg tggaggccaa cctgccccag ccgggggcgc cgcgcagaga
      121 cgactacgat ggcgacggct atatcatcgt ccggcatccg gagaccgagg tcgtgcacgc
      181 tgcggtgaag acgatcaccg agaccatcgg ggtcgagtac gctgatgagg cggacccccc
      241 acgccccgct gctccgagcg cgtcaggcag cgccaaacgg gtggacgacc actagaccat
      301 cgaggtaaag cggaagattt ccaattccaa ggactagagt cttccgactg gcgcatcacc
      361 cgccggcgcg ggatggtatt tccttgcgcc gtcctacacc gctgctggca ctctcgccgc
      421 aagtccagag ccggccacaa aggccatctt tttgtcccgg gcgcaccgcc tctattgcgt
      481 cgctggggtc aatcctctag tctcccccca tgcttcgaag gcgctttctt ggcgggctcg
      541 cggccgggct cgcgagtctc acagtcgtgc cgaggttgag ccgggccacc gtccggccac
      601 ggacgcctta tcacctgttc gtgctgctcg gtggcggtat tgacgcgatc ctatcgatgg
      661 atccaaagac caagagcgaa gtggatccgg cggtcgacct tccgtacgac gcacgcggca
      721 ttcattcgaa gggcgagctg gcgttcggac ccaacatggc gccgctcgca ccgtgggcgg
      781 agcacgccgc cgtcctgcgc ggtgtgctca cgagcagcgt cgcgcacatc ccggcgatag
      841 gccagctcgt gctcatgcga cgcgggattc cacctggcgg caagcacgtg gcgttcctcg
      901 atctcgtggg aggcgcgcat cggaccgcgc agctccccgt ggtccggttc gggaacgctg
      961 gcccggtagc tcccaagggg ctgatcacga gcgacaccgt gttcgaacgc ctgctgacgt
     1021 tgagccccga agagctcgac gtggttcgcc agcagctccg cacgcaggcg gactggctcg
     1081 ccggttcgcg cgaccgcgaa ggggcagcca ccgcatcgcg cctgcgcggc acggtcgagt
     1141 tcctggaccg cctgtctgcg ctgccgggtc cggcggccga ggcgtggacg accgcgcccg
     1201 aggcacagga gttcgcggag cagctccagc gatgcctgtg gttgttcgag cacgacctga
     1261 ccgcgtgtgc gctgatcaat caccagcgat gggatactca catcgacaac ttcgcggagc
     1321 agggtcggtt gtcgtcggcg ctgatgccgg tcctcgcgcg gtttgtcgag gagctcgcaa
     1381 agcgaacgaa ctcccacgga tcgatgctcg atcaggtcag ggtcatcgcc acgtccgagc
     1441 tcggacggtt tcccaagctc aacaccgagc gcggcaagca ccatttcccg gaggtcccga
     1501 tgctgttcat cggcgcgggc attccgggag gccacgcgtt cggacgtacc ggtcgcatga
     1561 tggaggcgct accgatggac ttgaacaccg ggcgcgccac cggcaacggt ggttcggccg
     1621 tcacgctcga cgacgtcggc gcaacgctgt tgcgatcggt gggtgtcgac ccggagctct
     1681 atggcaacag cgggcggcat ctgccgtttc tggtgccgtg atgcgccgca ggctcgttgc
     1741 tgtcgtgtgc ttccttgcgg cctgtcagac ccacgcaccg cgagcgacca ggcccgacgt
     1801 ccatgccgac gtcggacccg gcggcggagc aacacccgcc ggcggcggag caacacccgc
     1861 cggtggcgga gcaacactca aggtcgatcc cagacgcgcg aacgagatcg cggccgacct
     1921 gttcctcctc gcgtcgaacc gcgtgccgtc gcgccaggag ctcgccgagg cctccggagc
     1981 gctctcggca gggcagcagg agctccgcgc gttcgtcgcg gggctggtcg cgaccgacgc
     2041 cagcaccgcc ttcgcgtcgt tcgcgctgtt cccctgggag agcaaccccg ggaacccgcc
     2101 gcagggattc ggtcagctgt tcttcgagct gggcacgttc gaacaagcag gtcagaccgt
     2161 ccacttcctc accggcggca acgtcgtcgg cgacagcgtc gtcgccggca gcgccaaacc
     2221 cccgtgcacc ctgcgcgagg ccgagcgggt ccgcccgtgg tgggatcccg agcacgagat
     2281 cctggtgtgc cccagctcgc atcggccgga ccggatccgc gcgatcaacg gcaagcacaa
     2341 gctgtgctct ccggtgaccc cggagttcga gcgccagggc tgcggctgcg ggccgagcct
     2401 catgtactgc atcaagtccg agcggctcga tgatatttcc ttgagcttgc acaaggagtt
     2461 caacgacacg atcgcgtggg ccgtcgggca ggatcgcccg ctccaggatg tgtttcgcgg
     2521 ccgcgagacc gtgagggact ggttggtcga atggctctat cgccgccagc tggtggagag
     2581 cggtgcgccg gcggagcggt tgtcggctgc cggttgggcc ccgggccgct cgccgcgccc
     2641 ggagctccac ccgggggcgc acgccggctt gctgacgtcc ccccaggtgg cgctcctggg
     2701 ctgcgagcct cgcgaacgcc tgatgcagat ctcgaacttg atctggtgtg tcaagccccg
     2761 gagcgtcagc gtcaccgccg atacggtgtt caagctcccc accaacttcc gcgccccgat
     2821 gcaaggctgg cagttgctgg cgagcatgcc ggtctgcgac ggctgccatg cacggatgga
     2881 ctatggcgcg cagttcttcg cggggatgac cgccggcttt cactatgact ctcagatctc
     2941 gccgcacgcc ggcagcacga tgagcatgtt catgaacgac cagttcgacc tccgcggcaa
     3001 gggcgagcag actcccgccg ggttcgcgga gctcgcggtg tcgcaaccgg agttcggcgc
     3061 gtgcatgagc cagcgagtcg cggcgcatgt gttcggccag cacgcgaccg cggacgatgt
     3121 ggggtcgctg ctcgaagtgt tcaagacgcg gcccacgtac cgcgcgctct tgacctcggc
     3181 gctggtgtcg tacgcggagc gccggctgtg gagcgccggc gaacgcggcg agatcgctca
     3241 tgcggatccc gcggcggcgc agctcggcct cggcgctctg gtcgagcagt actgctccga
     3301 ttgccacgac gccggcagcc ccgccgagaa ggtatggaac tcgacccgag cgctcgccga
     3361 ccacggcgca atggtccagg cgctggattc catcggggac cggcggatgc cgcctgcccg
     3421 cgatccgctc gatcccgccg tgagacagcg attcgtcgcc ctgctcgccg aacggatctg
     3481 gcccgacacc gagccccgaa gcgccgcgct ggacaactac agcggacggc tggacaatgt
     3541 cgaggtccat cgccgggcct cgacgatgaa gatcatcggc gctctcgccg gtgtcgacct
     3601 cgacgatgcc atcgcgaagc tcgctgcgtt ctacgcgggc cgaggacgcg acgatcagga
     3661 gtacacgccc gacttcgcgc tcaacaccgt gatcgacggc gtcgcggcct gcaagcgcgc
     3721 cggcgccaag gacgtcatcg actgcgtcaa gcgcacgacc cgcctggacg cgatccttcc
     3781 ggtcgcgccg cgctgaccgg tccacctcca gcgatcgcca acgagggtga atcaaatgac
     3841 aaccgacacg caccacaggg agtttggcgc ctttcttcct tggcgttcgt tcgacttacc
     3901 tgtcgtaccg gtttcccacc cctggggaga cgactgtgtc ctcttcgggt ccggcgtgga
     3961 tgcatttcga ggtttgctcg agcacggccg ctccacgcgc ggctggcaac ggctctgggc
     4021 gccgagctac ttctgccaag aggtcatgcg gagccttctg accgcgggcc tccaggtcct
     4081 cctctatgac tacggccccg aagacacgtt cccgctgggc gccaggatca gtgccaaacc
     4141 gggtgacgtg gtcttgagaa tgaatttctt cggactccac gatcgcctct cgaccgatgg
     4201 gatcgatcgc aaccgcgtgg agatcctcga tgaccatacg cacgatccgt ggtcggactg
     4261 ggcatacacc agcgacgcgg actggtgcgt ggtatcacca aggaagaccc tgccgctccc
     4321 aggcggaggt gtgctctggt cgcctcgtcg gcatccactg cccacacctt cgcctgtcac
     4381 cgatacccgg cgagcagcca ctttcgagaa gttcactgca atggtgctca aggggctcta
     4441 cctgcagggc tatcccctgg acagggagat ctactggcgg ctgaacgacg cggccgaaga
     4501 tcatatggca tcaggtgaag cctcgggagt tccggagtgg atcggcgact tgctgaagtg
     4561 cttccctgtc cgggagtggc gcgagtatcg ccgcaacaac tgcctggccg tttcaaatgt
     4621 gctgaaaggt ctcccgtggc tgaaagtgct cgaagccgac gggggtggcg gaatctgtca
     4681 gttctcgggc ctcgtgctgt tcgactccga ggaacgccga aatttcgtca ggcggcagtt
     4741 ggccgaccgc cgcatcttcc tgacggtact gtgggaactg gatgatgctc cgattcccga
     4801 aatccccgag gaacaccgcc gcttcagcaa gaccctgctg tgtatccctt gcgacgcgcg
     4861 ctatgatcgg gtagccgtcg aaaaggtttc gggcctgatc cgggacattg gaaacgggtt
     4921 tgccagttct ggtcgggcgg cactgaacaa cgcctcgatc gagcaccagc agccgctggc
     4981 aaggccagcc ggcgcatcct ctccttgaag actcgcacct cgcgttccac atggatctac
     5041 caatgcggcc cccgatatcc gagcgcaagc tggtggtgct gatcggggct ggccagttca
     5101 tcaacacgct cgacttcacg atggtgctgc cgctgggccc cga
//
LOCUS       DASVOR010002365         2204 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_230570,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002365 DASVOR010000000
VERSION     DASVOR010002365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230570"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VF516_43595"
     CDS             <1..769
                     /locus_tag="VF516_43595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8114715.1"
                     /translation="EPAIKLIALSRNFGHQIAITAGMDASSGDAVVIMDADLQDPPSL
                     IPEMIRGYCEGYDVVYGQRTERDGETWFKRASAAAFYRLMRRLVDRRLPADTGDFRLV
                     SRRVIEALRQVREHDRFVRGIVAWLGFAQRAVPYRRPVRAAGTTKYPLTKMLRLALDA
                     MISFSNLPLRLITWFGAAAAAASTVALVGALWVDVRSGAGFGWAEVAALVGWFGGAIL
                     FAIGVLGIYVYKIHTEVRARPLYLVRHSMNLDREPRA"
     gene            766..1743
                     /gene="galE"
                     /locus_tag="VF516_43600"
     CDS             766..1743
                     /gene="galE"
                     /locus_tag="VF516_43600"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEX8114716.1"
                     /translation="MTVLVVGGAGYVGSHAVRALAAAGTRVVVLDDLSTGHRKLVPAG
                     VPLHVGRLEDRDRLVELLVGERVRAVVHCAACSLVAESETNPRKYFERNCGGVLALTG
                     AMLDAGVTDLVLSSSAAVYGEADVAVLDESQPRRPSSVYGVTKAFAEDVLTAYRAHGL
                     RSVALRYFNAAGADPSGEIGEDHRHETHLLPIVLDAVLGFREAVTVFGIDYPTRDGTC
                     LRDYVHVSDLADAHLRAIDHLRGGGASMQLDLGSTTGTTVREVIAAVEAVTGQRVPVR
                     DGARRPGDPARLVTSAQRAEQVLGWRARRPDIHTIVEDAWRWHRRLRAA"
     gene            1740..>2204
                     /locus_tag="VF516_43605"
     CDS             1740..>2204
                     /locus_tag="VF516_43605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114717.1"
                     /translation="MNRRAVLALLGLCVALRAVSLVRPCLSDDEAIYATVAREMLTGH
                     ELYRDVVDHKPPGVYLVYAATQAIGGPIGGMVVLHLLLIGAVFATGLLLARFVRLHWP
                     GTDPRAPAAAALLWIVYTTTLVDVDALAANCELFMMLPLVGAVVVFLDARGRL"
BASE COUNT          251 a          745 c          874 g          334 t
ORIGIN      
        1 ggagccggcg atcaagctga tcgcgctgtc gcgcaacttc gggcaccaga tcgcgatcac
       61 ggcggggatg gatgcgtcgt cgggcgatgc cgtggtgatc atggacgccg acctgcagga
      121 cccgccgtcg ctgatcccgg agatgatccg cggctactgc gagggctacg acgtggtcta
      181 cggccagcgc accgagcgcg acggcgagac ctggttcaag cgggcgagcg cggcggcgtt
      241 ctaccggctg atgcggcggc tggtcgatcg ccggctgccg gccgacaccg gggacttccg
      301 gctggtcagc cggcgggtga tcgaggcgct gcgccaggtc cgcgagcacg accggttcgt
      361 gcgcggcatc gtcgcctggc tggggttcgc gcagcgcgcg gtgccgtatc gccggccggt
      421 gcgggcggcg gggacgacca agtacccgct gaccaagatg ctgcggctgg cgctcgacgc
      481 gatgatcagc ttctccaacc tgccgctgcg gctgatcacg tggttcggcg cggcggcggc
      541 ggcggcgagc acggtggcgc tggtcggcgc gctgtgggtc gatgttcgct ccggggccgg
      601 gttcggctgg gcggaggtcg cggcgctggt cggctggttc ggcggcgcga tcctgttcgc
      661 gatcggcgtg ctggggatct acgtctacaa gatccacacc gaggtgcgcg cccggccgct
      721 gtacctggtg cgccattcga tgaacctcga ccgggagccg cgcgcgtgac ggtgctggtc
      781 gtcggcggcg ccgggtacgt cggctcgcac gcggtgcgcg cgctggccgc ggccgggacc
      841 cgcgtggtcg tgctcgacga cctgtcgacc ggccatcgca aactggtgcc cgccggcgtg
      901 ccgctgcacg tcggccggct cgaggaccgc gaccggctgg tcgagctcct ggtcggcgag
      961 cgggtccgcg cggtcgtgca ctgcgccgcg tgctcgctgg tcgccgagtc ggagaccaac
     1021 ccgcgcaagt acttcgagcg caactgcggc ggggtgctgg cgctgaccgg cgcgatgctc
     1081 gacgccgggg tgaccgatct cgtgctgtcg tcgagcgcgg cggtgtacgg cgaggccgac
     1141 gtggcggtgc tcgacgagag ccagccgcgc cggccgtcca gcgtgtacgg cgtgaccaag
     1201 gcgttcgccg aggacgtgct gaccgcgtac cgggcccacg gcctgcgctc ggtcgcgctg
     1261 cgctacttca acgccgcggg cgccgatccg tcgggcgaga tcggcgagga ccaccggcac
     1321 gagacccacc tcttgccgat cgtgctcgac gcggtgctcg ggttccgcga ggcggtgacg
     1381 gtgttcggca tcgactatcc gacgcgcgac gggacgtgcc tgcgcgacta cgtgcacgtg
     1441 tcggacctgg cggacgccca cctccgcgcg atcgatcacc tgcggggcgg cggcgcgtcg
     1501 atgcagctcg atctcggctc gaccaccggc accacggtgc gcgaggtgat cgcggcggtc
     1561 gaggcggtga ccgggcagcg cgtgccggtc cgcgacggcg cgcggcggcc gggcgacccg
     1621 gcccggctgg tcacctcggc gcagcgcgcc gagcaggtcc tgggctggcg cgcgcggcga
     1681 ccggacatcc acacgatcgt cgaggatgcc tggcgctggc accgccggct gagggcggcg
     1741 tgaaccgccg cgcggtgctc gcgctgctcg ggctgtgcgt cgcgctgcgc gcggtgtcgc
     1801 tggtgcggcc gtgcctgtcc gacgacgagg cgatctacgc gacggtggcg cgcgagatgc
     1861 tgaccggcca cgagctgtat cgcgacgtgg tcgatcacaa gccgcccggg gtctacctgg
     1921 tgtatgcggc gacccaggcg atcggggggc cgatcggcgg catggtcgtg ctccacctgc
     1981 tgctgatcgg cgcggtgttc gcgaccgggc tgttgctcgc gcggttcgtg cggctgcact
     2041 ggccggggac cgatccgcgg gcgccggcgg ccgccgcgct gctgtggatc gtgtacacga
     2101 cgacgctggt cgacgtcgac gcgctggccg ccaactgcga gctgttcatg atgctgccgc
     2161 tggtcggcgc ggtggtggtg ttcctcgacg cgcgcggccg gctc
//
LOCUS       DASVOR010002366         2148 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_287763,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002366 DASVOR010000000
VERSION     DASVOR010002366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2148)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2148)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2148
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287763"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..366)
                     /locus_tag="VF516_43610"
     CDS             complement(103..366)
                     /locus_tag="VF516_43610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114718.1"
                     /translation="MTDRGYGHGSAEQEGRTRGHVTETSAEPPGVGKVSLIQLRFGPN
                     TPLPNESGKRTLTEQVVPIQAQRPEPGNATGVNVTILGEGYRA"
     gene            complement(510..947)
                     /locus_tag="VF516_43615"
     CDS             complement(510..947)
                     /locus_tag="VF516_43615"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8114719.1"
                     /translation="MTLLDRLTAARASQDYQALVDLIPYASFLGLTAAMQGDELITTM
                     RYGAHLIGNPALPALHGGTLGALLESAAVFELLFRSETVVLPKTITLTVDYLRSGAPV
                     DTHARGVVTRQGRRVTNVRMEAWQTDRGKPVATAHAVFLVMRS"
     gene            complement(950..1363)
                     /locus_tag="VF516_43620"
     CDS             complement(950..1363)
                     /locus_tag="VF516_43620"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEX8114720.1"
                     /translation="MARAFSGGVPHNLALGLRVLGVSPAEASFELPYDARLVGNPDTG
                     TLHGGAITALLDACSGAAVFAALPSLVPIATLDLRIDYLKPAEPGKPVIAKASCYKLT
                     RNVAFTRGVAYQDDPENVIAHSVGTFMLSTKLGNV"
     gene            1512..2009
                     /locus_tag="VF516_43625"
     CDS             1512..2009
                     /locus_tag="VF516_43625"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEX8114721.1"
                     /translation="MAHAASPDRRRSPTVPPRGPIPTWYFALVVVRRGHRFLLTQERK
                     YGATWSIPGGRVEPGETLASAAIREVLEETGVPAKLDGVLRVEHSPGEASARIRVIFL
                     GSPLDDTEPKTVPDDESLGAAYLTLDEIRERPLRGAELRVLLETVADGCQVFPLELLG
                     TEMSV"
BASE COUNT          295 a          807 c          729 g          317 t
ORIGIN      
        1 tgggcccagc gcgatgccag gctggaagcc gcccgcgagg ttcggcgcct gcgccgcgcc
       61 gagctccacc aggaggcggc atgacccttc tggttccctg cttcaggctc tgtaaccttc
      121 acccagtatc gtcacgttca ctccggtcgc gttgcctggc tcgggacgct gcgcctggat
      181 cggaacgacc tgctcggtca gcgtgcgctt gccggactcg ttcggcagcg gcgtgttcgg
      241 cccgaaacgg agctggatca gcgatacctt gccgacgccc ggcggctcgg ccgatgtttc
      301 cgtcacgtgc ccgcgggtgc ggccttcctg ctcggcggat ccgtgaccgt accctctgtc
      361 cgtcatacgc ctcacctcgc ggcagcctac cgcaagcatg ctctcgacgc ctcgccgacg
      421 cgggtgagac ctacaccccg cccgatggcc acgacaccgg ccgcacctgc cgatgcacgg
      481 ttccgcgtgc tcgccggcgg gcccctgacc tagctgcgca tcaccaggaa cacggcgtgc
      541 gcggtcgcga ccggctttcc gcggtcggtc tgccaggcct ccatccgcac gttggtgacc
      601 cggcggccct ggcgggtgac cacgccgcgc gcatgggtat cgaccggcgc gcccgaccgc
      661 aggtagtcga cggtcagcgt gatcgtcttg ggcaggacca cggtctcgga ccggaacagc
      721 agctcgaaca ccgccgccga ctccagcagc gcgccgagcg tgccgccgtg cagcgccggc
      781 agcgccgggt tgccgatcag gtgggcgccg tagcgcatgg tcgtgatcag ctcgtcgccc
      841 tgcatcgcgg cggtcaggcc gaggaagctg gcgtagggga tcaggtcgac gagcgcctgg
      901 tagtcctgcg acgcgcgcgc cgcggtgagc cggtcgagca gcgtcatgct cacacgttcc
      961 cgagcttggt cgacagcatg aacgtgccga ccgagtgcgc gatcacgttc tcgggatcgt
     1021 cctggtacgc aacgccccgc gtgaacgcca cgttgcgcgt cagcttgtag cagctcgcct
     1081 tggcgatcac cggcttgccg ggctcggccg gcttgaggta gtcgatgcgc aggtcgagcg
     1141 tcgcgatcgg caccagcgac ggcagcgcgg cgaacaccgc cgctccggag cacgcgtcga
     1201 gcagcgccgt gatcgcgccg ccgtgcagcg tcccggtgtc ggggttgccc accagcctcg
     1261 catcgtacgg cagctcgaag ctcgcctctg cgggcgagac accgaggacc cggagcccga
     1321 gcgcgaggtt atgcggcacc ccgcccgaga atgcgcgagc cattttcacc tgcatgtcgt
     1381 cgccggcatc cgccatcacc gccgacggta tcaggaaacg gcatgaggaa cgcgcatcga
     1441 accggactcg ctccgccttg ggtctcgagg tcgcacgccg cgccggcggc gctcatggta
     1501 attgtgagtc catggcgcac gcagccagtc cggaccgccg ccgttccccc accgtgccgc
     1561 cacgtggacc gatcccgacc tggtacttcg cgctcgtcgt cgtgcgccgc ggccaccgct
     1621 tcctcctgac ccaggaacgc aagtacggcg ccacgtggtc gatccccggc gggcgggtcg
     1681 agcccggcga gaccctggcg tcggccgcga tccgcgaggt gctggaggag accggcgtcc
     1741 cggccaagct cgacggcgtg ctgcgcgtcg agcactcccc cggcgaagcc agcgcgcgca
     1801 tccgcgtgat cttcctcggc tcgccgctcg acgacaccga gcccaagacc gtcccggacg
     1861 acgagtcgct gggcgcggcc tacctgacgc tcgacgagat ccgcgagcgc ccgctgcgcg
     1921 gcgccgagct ccgcgtgctc ctggagaccg tcgccgacgg ttgccaggtg ttcccgctcg
     1981 agctcctggg caccgagatg tccgtgtgac gcacgcgccc gcgccggccg acgccctgct
     2041 tcggctgggc tgggacaccg catggcaggc cgcgcgcgcc gctgccgatc ccgacgccgc
     2101 ctggcagccc gcgcggatcg ccgccgagca ccgcggcgcg taccaggc
//
LOCUS       DASVOR010002367         2659 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_434367,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002367 DASVOR010000000
VERSION     DASVOR010002367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434367"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1026)
                     /locus_tag="VF516_43630"
     CDS             complement(139..1026)
                     /locus_tag="VF516_43630"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEX8114722.1"
                     /translation="MNASSEDHCTGLARGSRFVVVAAAMLVSRAAVAQAVAQAVIETP
                     APSPKARVEQRVGITDVSIDYSSPGVKGRKIWGELVPYDKVWRAGANAPTKLTVSRDF
                     TFGGAAVKAGSYAVFMTPGKKTWTVALNTDLTASQDSHDDKNDVARAQVTPAALPALR
                     ERLVYLLTDTQDDRTNLDLEWERVRIRVPITIDTRALVAAGIEKALAAAWQPHATAAS
                     YYFNAGDLARALALVDKSIAIESNWRNEWLRAQIQGKKGNKAEAVASAHRALAAGKGD
                     ANFEQNVQPNIVKALAGWK"
     gene            1254..1514
                     /locus_tag="VF516_43635"
     CDS             1254..1514
                     /locus_tag="VF516_43635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114723.1"
                     /translation="MDGVDETPTGDGAYLLRCTLGASRNPAPAIERMRQRAGELCPGG
                     FEVLDSADGSRSTYVRTVHGVEAIRRSDVALVVRCHPPRTAD"
     gene            1744..>2659
                     /locus_tag="VF516_43640"
     CDS             1744..>2659
                     /locus_tag="VF516_43640"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114724.1"
                     /translation="MGTVYVGEHTLIGRRAAIKVLDPELSRQREVVDRFFNEARAAAA
                     VKHPGVVQIFDFGFTDDGSAYLVMELLEGRSLAAVLRETGALPVRDALHVIRQVAWST
                     AAVHAVDVIHRDLKPDNIHLVRDPDAMGGLRVKILDFGVAKLGDAFQPSLTVSGRIYG
                     TPLYMAPEQFVGKVDVRSDVYAAGCMLFEALVGKPPFQGATLIELGLAHTNAPPPAPS
                     SVCSAIAPDVDAIVLRCLAKDPAQRFSSMIELAQAIEGQLGGQIEDHLDLPAERVAKA
                     VGPAHEAPGEPAVEASAPPPGELPAGSPP"
BASE COUNT          348 a          978 c          930 g          403 t
ORIGIN      
        1 cagcggccgg cgcgcccggg ccacggcgcg tgcgagcgcg gccgccgaca agggtggcgc
       61 gccgggttct tcgtcgtcgg gctgcagcgc gcgcacctcg cctccgaggt acgctcgcac
      121 gaggtcacgg ctcacgcgct acttccagcc ggcgagcgcc ttgacgatgt tgggctggac
      181 gttctgctcg aagttcgcgt cgcccttgcc ggccgcgagc gcgcgatggg ccgacgccac
      241 cgcctcggcc ttgttgccct tcttgccctg gatctgggcg cgcagccact cgttgcgcca
      301 gttcgactcg atcgcgatcg acttgtcgac cagcgccagc gcgcgcgcca ggtccccggc
      361 gttgaagtag tagctcgccg cggtcgcgtg cggctgccac gccgccgcga gcgccttctc
      421 gatccccgcg gccaccaagg ccctggtgtc gatcgtgatg ggcacccgga tccgcacgcg
      481 ctcccactcc agatcgaggt tggtgcgatc gtcctgggtg tcggtcaaca gatacaccag
      541 ccgctcgcgc agcgccggca gcgcggccgg cgtcacctgg gcgcgcgcca cgtcgttctt
      601 gtcgtcatgg ctgtcctggc tcgccgtcag gtcggtgttg agcgccaccg tccacgtctt
      661 cttgcccggc gtcatgaaca ccgcgtagct gcccgccttg accgcggcgc cgccgaacgt
      721 gaaatcgcgg ctcaccgtca gcttggtcgg cgcgttggcc ccggcccgcc acaccttgtc
      781 gtacggtacc agctcgcccc agatcttccg ccccttcacg cccgggctcg agtagtcgat
      841 cgacacgtcg gtgatcccga cccgctgctc gacccgcgcc ttggggctcg gcgccggcgt
      901 ctcgatcacc gcctgggcga ccgcctgcgc gaccgccgcg cgcgacacca gcatcgcggc
      961 ggcgacgacg acgaagcgac tcccccgggc caggccggta cagtggtcct cggacgatgc
     1021 gttcatgccc gcacactaca gccgtagtaa gcgatcgcat tcccccaggg cctggttttt
     1081 tcggccacgg cgtgtcatgc ggcgaggcac ggaagccgca cgcccgtcgc gcaccacgcc
     1141 gcacgccggt cgcacccgtc gctgtaccgg tacctgctgt gcctgcgctg gccgcggcga
     1201 tcgcgctggc agcggggtgc ctcgcgccgg gtgccggtac ccgcgttgcc ggggtggatg
     1261 gcgtcgatga gacccccacc ggcgacggcg cctacctcct gcgatgcacg ctcggcgcgt
     1321 cccggaatcc agcgcccgcg atcgagcgca tgcgccagcg tgccggcgag ctctgcccgg
     1381 gaggcttcga ggtcctcgac agcgccgatg gttcgaggtc gacctacgtc cgcacggtcc
     1441 acggcgtcga ggcgatccgt cgctccgacg tcgcgctcgt ggtgcggtgt catccacccc
     1501 gcaccgccga ttgacgtcgc gtcgccgagg caggtgccgc cctgcgcgac agccggtgtt
     1561 ggcggtgcaa gcgggcgtac gacggaaagc ccgcccgacg aggcgcggcg gccccgtctt
     1621 gcgccgccgt gaccgcagat ccatcatcga tcgcgaaggg ggctcatcct cgaacaaggg
     1681 ggtcgcttgg taggtaccct gatcgggcag taccggatcg tgagcgccgt cggatccggc
     1741 ggcatgggga cagtctacgt cggggaacac acgctgatcg gccggcgggc cgcgatcaag
     1801 gtgctcgacc ccgagctgtc gcggcagcgc gaggtcgtcg atcggttctt caacgaggcg
     1861 cgggccgcgg cggcggtcaa gcacccgggc gtcgtgcaga tcttcgactt cgggttcacc
     1921 gacgacggca gcgcgtacct ggtcatggag ctgctcgagg gccggagcct ggccgccgtg
     1981 ctgcgcgaga ccggcgcgct gccggtgcgc gacgcgttgc acgtgatccg ccaggtcgca
     2041 tggtcgaccg ccgcggtgca cgcggtggac gtcatccacc gcgacctcaa gcccgacaac
     2101 atccacctgg tccgcgatcc ggacgcgatg ggcggcctgc gggtcaagat cctcgacttc
     2161 ggcgtcgcca agctcggcga cgcgttccag ccgtcgctca ccgtgagcgg ccggatctac
     2221 gggacaccgc tgtacatggc ccccgagcag ttcgtcggga aggtcgacgt gcgcagcgac
     2281 gtctacgcgg ccggctgcat gctgttcgag gcgctggtcg gcaagccgcc gtttcaaggc
     2341 gccacgctca tcgagctcgg gctcgcgcat accaacgcgc cgccgccggc gccgtcgagc
     2401 gtgtgctcgg cgatcgcgcc ggacgtcgac gcgatcgtgc tgcgctgcct ggccaaggac
     2461 ccggcgcagc ggttctcgtc gatgatcgag ctcgcccagg cgatcgaggg ccagctcgga
     2521 ggccagatcg aagaccacct ggatctgccg gcggagcggg tggcaaaggc cgtcggcccc
     2581 gcgcacgagg ccccgggtga gccggcggtg gaggcgagcg cgccgccgcc gggtgagctc
     2641 cccgccggct caccgccgg
//
LOCUS       DASVOR010002368         4983 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_201416,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002368 DASVOR010000000
VERSION     DASVOR010002368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4983
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201416"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..812)
                     /locus_tag="VF516_43645"
     CDS             complement(<1..812)
                     /locus_tag="VF516_43645"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114725.1"
                     /translation="MLDPWIMPPTAALGFQPGEVIDGRYVLRDRLGEGGMGTVFLANQ
                     PGLERMVAIKVLHPELVGEPVHARRLREEARIACRVRSASCVGMIDCGELPDGAPYLV
                     MEYVPGEVLGQVLAEQSISLSRAVEVMEQILGALGAVHAAGIVHGDVKTDNFLVETVA
                     GRDRVTLIDFGLARLADAPHEVDMEDGEVMVSGTPEYMAPEVVAGEPPGRAADLYAAG
                     VILYELLTGTTPFAGGAPEAIMLRHAREAVIPPSLRCPERDLPPALDRVVLRA"
     gene            complement(980..1402)
                     /locus_tag="VF516_43650"
     CDS             complement(980..1402)
                     /locus_tag="VF516_43650"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEX8114726.1"
                     /translation="MTQQIKDVMTKQVQCVRKDATLRAVARVMKEHKIGAVLVTQADG
                     SLLGLVTDRDLVVRGMASDRDIDKTQVGEICSTPPVKLDPTATLDDAVRLMRERTVRR
                     IPVVADGKPIGIVSIGDLARAKDPNSLLAQISAAPPNE"
     gene            complement(1545..2210)
                     /locus_tag="VF516_43655"
     CDS             complement(1545..2210)
                     /locus_tag="VF516_43655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114727.1"
                     /translation="MRKWVFMISLARSRNRITGLGVWRLALIGLAVAACAGPHTSVEL
                     AWTAPNAESAQLHRVVTLFVSRDVALRRPAEDEMARQLVERGVEARPAYQLLSRDDLS
                     NLNAAKAKLRALGYDGVVTMRIVGEEQTLNYVPPSFDAYWTWASPYFFWPGYYAPGTY
                     SPGYVYTTTIVRMETEAYSLTDDRLVWAALSKTTDPGNARKLIDDVTRTVATKLTDQG
                     LVA"
     gene            2327..3091
                     /locus_tag="VF516_43660"
     CDS             2327..3091
                     /locus_tag="VF516_43660"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX8114728.1"
                     /translation="MHAASDMTGEVDPAGAQRVERRSSTGDEPAPPRRSSRRERLELL
                     VFLLLVLPPIVLGSVAEGSGQAFTITGLATILHDVALCALILFFLWTAGERFSAIGWT
                     SRRLGREIALGAALYLPVLAAITAVGWLVQAAGLEAPRSPPAALVPRSPADIVLAAVL
                     VIVVAVAEETIFRGYLVLRLRAVTHSTAAAVALSTLLFAAGHTYSGAAGVVVVSVLAV
                     VLALIYLWRGSLVAPIVLHLLQDLLGLILAPWLGLR"
     gene            3292..3726
                     /locus_tag="VF516_43665"
     CDS             3292..3726
                     /locus_tag="VF516_43665"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEX8114729.1"
                     /translation="MNQSPAANGDGATTRDLIRRISEDLRTITRNEIELLRGEARRVA
                     RSAAADGAVVVFGGTVALIGLAILCLAAVAALAPVLPSLALRLVVMGVVYLGIGGSLA
                     ATFALRLRRNAVPDIAIPVREVRATLKGAKATIEERGRQLHA"
     gene            3719..3949
                     /locus_tag="VF516_43670"
     CDS             3719..3949
                     /locus_tag="VF516_43670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114730.1"
                     /translation="MRDRTAIEREILDAREDLEENLDRLMHRARERLSLRARAGHALR
                     ELPREYPNLTTVAVIACVIVLLLVRRAQRRRA"
     gene            complement(3962..>4983)
                     /locus_tag="VF516_43675"
     CDS             complement(3962..>4983)
                     /locus_tag="VF516_43675"
                     /inference="COORDINATES: protein motif:HMM:NF040249.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HEX8114731.1"
                     /translation="GERFGIDGARLVRAVALGYDVGPRVTMALGGLPFQIKTHHSAHS
                     IAGTFGAAAAAACAAGLSAQQMRWVLDYAAQQASGVAAWQRDQDHIEKALVFGGFPAR
                     NGVSAALIIQLGGTGVADIFSGDDNFLLTFAPGADPGPLIDKLGERFEVVRTNIKKWT
                     VGSPIQAPLDALELLHKRRPFTADEVQSVAVRVATSEAKTVNDREMRSISLQHMVAVM
                     LIDHTITFAAAHDKRRLQDPTVLRHRAKVRLVPDEELEKLYPQRVAIVEVALADGTKL
                     VERVDAVRGTSDNPMTRDEVATKVRDLTAPVLGAGRAARLIETVWTLEAVKDVRSLRP
                     LLQRR"
BASE COUNT          670 a         1854 c         1713 g          746 t
ORIGIN      
        1 gcgcgcagca cgacgcgatc gagcgcgggc ggcagatcgc gctcggggca gcgcagcgac
       61 ggcgggatca cggcctcgcg ggcgtggcgg agcatgatcg cctccggcgc gccgccggcg
      121 aacggcgtgg tcccggtcag cagctcgtag aggatcacac cggcggcata gaggtcggcg
      181 gcgcggcccg gtggctcgcc ggcgaccacc tcgggcgcca tgtactcggg ggtgcccgac
      241 accatcacct cgccgtcctc catgtcgacc tcgtgcggcg cgtccgcgag ccgcgcgagc
      301 ccgaagtcga tcagcgtgac gcgatcgcgg cccgcgacgg tctcgaccag gaagttgtcg
      361 gtcttgacgt cgccgtggac gatgccggcg gcgtggaccg cgccgagcgc gcccaggatc
      421 tgctccatca cctccaccgc gcgcgacagc gagatcgact gctcggcgag gacctggccg
      481 agcacctcgc cgggcacgta ctccatcacg aggtacggcg cgccgtcggg caactcgccg
      541 caatcgatca tcccgacgca gctcgcgctt cgcacccggc acgcgatccg ggcctcctcg
      601 cgcagccgcc gggcgtgcac cggctcgccg accagctcgg gatgcaggac cttgatcgcg
      661 accatgcgct ccaggccggg ctggttggcc aggaacacgg tccccatgcc gccctcgccg
      721 aggcgatcgc gcagcacata gcggccgtcg atcacctcac ccggttgaaa ccccagcgct
      781 gcagtcggcg gcatgatcca ggggtccagc aagcggcata ccgaagctcc aggatgctcc
      841 tacaagcatc tacgcgtgtt gcgcatcgcg ccatccgaca gttttgccgg cctgtcggta
      901 tgccacgcag cccttgcgcg atcgccgtcg ccagcgtcca tctccgcgca catctggcgg
      961 gccgacgcca gaggatccgt cactcgttcg gtggggccgc gctgatctgg gccagcagcg
     1021 agttggggtc cttggcgcgg gcgagatcgc cgatcgacac gatgccgatc ggcttgccat
     1081 cggccacgac cggaatgcgg cggaccgttc gctcccgcat cagccgcacg gcgtcgtcga
     1141 gcgtggcggt gggatcgagc ttgaccggcg gcgtggagca gatctcgccg acctgcgtct
     1201 tgtcgatgtc acgatcggac gccatcccgc gcacgacgag atcgcgatcg gtcacgagcc
     1261 cgagcagcga gccgtcggcc tgcgtgacca gcacggcgcc gatcttgtgc tccttcatca
     1321 cgcgagccac cgcgcgcagc gtcgcgtcct tgcgcacgca ctggacctgt ttcgtcatca
     1381 catctttgat ctgctgagtc atggcaacct cctgagtccg gacggggctg caaggcatac
     1441 gcccggcgcg gcgatgtggc ggctgcggtc ccggcgcggt tcgagcagcg cagcacgcct
     1501 cgcggcgcgc accgcgcgcg cccgcgccac gcgcggatcc accgctaggc gaccaggccc
     1561 tgatcggtca acttggtcgc cacggtccgg gtgacgtcgt cgatcagctt gcgtgcgttg
     1621 ccgggatccg tggtcttgct cagcgccgcc cacaccaggc ggtcgtcggt cagcgagtac
     1681 gcctcggtct ccatgcgcac gatggtcgtc gtgtagacat agcccggcga gtaggtacca
     1741 ggtgcgtagt agcccggcca gaagaagtag ggcgaggccc aggtccagta tgcgtcgaag
     1801 ctcggcggca cgtaattgag agtctgctcc tcgccgacga tgcgcatggt cacgacgccg
     1861 tcgtagccca gcgcgcgcag cttggccttg gcggcgttca ggttgctcag gtcgtcacgg
     1921 ctgagcagct ggtacgccgg cctggcctcg acccctcgct ccacgagctg gcgcgccatc
     1981 tcgtcctcgg ccgggcgccg cagcgcgacg tcccgcgaca cgaacagcgt cacgacccgg
     2041 tggagctgtg cgctctccgc gttgggcgcc gtccacgcga gctcgacgct cgtgtggggt
     2101 cccgcgcagg ccgcgacggc gagcccgatc agcgcgagcc gccatactcc gagacccgtg
     2161 atccggtttc gactgcgagc aagggagatc atgaataccc actttctcat cgacgtgcga
     2221 ttggatctcc gacggagcaa tgcgcgtgcc acgcgcgcac ttcgccgccg cgtcgcgtgc
     2281 gatcggcgca cagccacgcc cgcggggccg cgctatggtc gcccgcatgc atgctgcatc
     2341 ggacatgacc ggcgaggtcg accccgcagg cgcgcagcgg gtggagcggc gatcgtccac
     2401 cggcgacgag ccggcgccgc cgcggcgcag ctcacgccgc gagcgcctcg agctcctggt
     2461 gttcctgctc ctggtcctgc ccccgatcgt cctgggctcg gtggccgagg gctcgggcca
     2521 ggcattcacg atcaccggcc tggcgacgat tctccacgac gtcgcgctgt gcgcgctgat
     2581 cctgttcttc ctgtggaccg ccggagagcg gttctcggcg atcggctgga cgtcgcgccg
     2641 cctcggccgc gagatcgcgc tcggcgcagc gctgtatctt ccggtgctgg ccgcgatcac
     2701 cgccgtcggc tggctggtgc aggccgccgg cctcgaggcg ccgaggtcgc cgcccgccgc
     2761 cctggtgccg cgctcgccgg ccgatatcgt gctcgccgcc gtgctggtga tcgtcgtcgc
     2821 cgtcgccgag gagacgatct tccggggcta cctggtgctc cggctgcgcg ccgtcacgca
     2881 cagcacggcc gccgccgtcg cgctatcgac gctgctgttc gctgcgggac atacctacag
     2941 cggcgcggca ggcgtcgtgg tcgtgtccgt gctcgccgtc gtgctggccc tgatctacct
     3001 gtggcgaggc agcctggtcg ccccgatcgt gctgcacctg ctccaggacc tcctcggcct
     3061 gatcctcgcg ccctggctcg gcctgcgctg acgccggcgc gcggccgacc gatccgcccg
     3121 ctgcccggcg gcgcgctgtg acaggacgac cgtgcgcctg gctccggtgc cggaaccccg
     3181 gaccgatcga cggcgccgtg gcgactccgc ggatcacgac gcgatcaccg cgttgccggc
     3241 acggcgcggc gcgacgcccc gcgcggcaca tcgcctgcac cctcgggacg catgaaccag
     3301 agcccagcgg caaacggcga tggggcaacg acgcgagacc tcatccgccg gatcagcgag
     3361 gatctacgaa cgatcacgcg gaacgagatc gagctgttgc gcggcgaggc tcgtcgcgtc
     3421 gcgcggtccg ccgcggccga cggcgccgtc gtcgtgttcg gcggcaccgt ggcgctgatc
     3481 gggctcgcga tcctgtgcct ggccgcggtg gcggcgctcg cccccgtgct gcccagcctc
     3541 gcgctgcggc tcgtcgtgat gggcgtcgtc tacctcggca tcggcggctc gctggcggcg
     3601 accttcgcgc tgcgcctgcg acgcaacgcc gtccccgaca tcgcgatccc ggtccgcgag
     3661 gtcagggcca cgcttaaggg cgcgaaggcc acgatcgagg agcgcgggag gcagctccat
     3721 gcgtgaccgc accgcgatcg agcgcgagat cctcgacgcc cgcgaggacc tcgaggagaa
     3781 cctcgaccgg ctgatgcacc gggcgcgcga gcggctgtcc ctgcgagcgc gcgccggcca
     3841 cgcgctccgc gagctcccgc gcgagtaccc caacctcacg accgtggcgg tgatcgcgtg
     3901 cgtgatcgtg ctcttgctcg tgcgacgcgc ccagcgccgc agggcgtgac gccggccgcg
     3961 atcaccgccg ctgcagcagc gggcgcaggc tgcggacgtc cttcacggcc tccagcgtcc
     4021 acacggtctc gatcaggcgc gcggcgcggc ccgcgccgag caccggcgcc gtcagatcgc
     4081 gcaccttcgt ggcgacctcg tcgcgggtca tcgggttgtc cgacgtgccg cgcaccgcgt
     4141 cgacccgctc gaccagcttc gtgccgtcgg ccagcgcgac ctcgacgatg gcaacgcgct
     4201 gcggatacag cttctccagc tcctcgtccg gaaccagccg caccttggcc ctgtggcgca
     4261 gcacggtggg gtcctgtagc cgccgcttgt cgtgagcggc cgcgaacgtg atcgtgtggt
     4321 cgatcagcat caccgccacc atgtgctgga gcgagatcga tcgcatctcg cggtcgttca
     4381 ccgtcttggc ctcgctggtc gccacccgca ccgccacgct ctgtacctcg tcggcggtga
     4441 acggccggcg cttgtggagc agctcgagcg cgtcgagcgg cgcctggatc ggcgagccga
     4501 ccgtccactt cttgatgttg gtccgcacga cctcgaagcg ctcgccgagc ttgtcgatca
     4561 gcgggcccgg gtcggctccc ggcgcgaatg tcagcaggaa gttgtcatcg cccgagaaga
     4621 tgtccgcgac gccggtgccg ccgagctgga tgatcagcgc cgccgacacg ccgttgcgcg
     4681 ccgggaaccc gccgaacacc agcgccttct cgatgtggtc ctggtcgcgc tgccacgcgg
     4741 ccacgccgga cgcctgctgc gccgcgtagt cgagcaccca tcgcatctgc tgcgccgaca
     4801 gccccgccgc gcacgcggcc gccgccgcag cgccgaacgt gcccgcgatg ctgtgcgcgc
     4861 tgtggtgggt cttgatctgg aacggcaggc cgccgagcgc catcgtcacc cgcggcccga
     4921 cgtcgtagcc gagcgcgacc gcccgcacca gccgcgcgcc gtcgatcccg aaccgctcgc
     4981 cga
//
LOCUS       DASVOR010002369         2556 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_552653,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002369 DASVOR010000000
VERSION     DASVOR010002369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2556
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552653"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..1409
                     /locus_tag="VF516_43680"
     CDS             213..1409
                     /locus_tag="VF516_43680"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF005154.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEX8114732.1"
                     /translation="MTPTGIVVQKYGGSSVADADKIKLVAKRIARTKAAGKKVVVVVS
                     AMGKTTNQLIEKARSISESPSRRELDMLLTCGEREAMALLSMACSEEGLEAISFTGSQ
                     AGILTNDRHSGARIVEVRPFRIEDELDRGKVVIVAGFQGVSYKREITTLGRGGSDTTA
                     VALAAALRADYCEICSDVDGVYTADPRVVDAARLLESISHDEMLELAAQGAKVLHDAS
                     VEFARKSGIALYARATGKDGGGTRIDFSPDRERQVAAVTGQSQLIRIRIAGGAGGAGG
                     AAVEHCLQILETASIPLTHLDLEGQELRAWFTIADVPDWGEVRKRLDNVLGDALELGE
                     EGAVTMVGHGIGGNPGIINKARATAVGAGVPLNAVSVSPLRVTLWCDRPHVDHLVRAL
                     HRTFIE"
     gene            1439..2227
                     /locus_tag="VF516_43685"
     CDS             1439..2227
                     /locus_tag="VF516_43685"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEX8114733.1"
                     /translation="MPKPPLARLLPHLGRLGRATRWRRTRGRPRVWAHRGDSAHAPEN
                     TMLAFDRARAAGADGIELDVRFDGDGNVVVFHDSQLERLTGQPGRMEDLSASARAALR
                     VGGEPVPLLAEALAGFDLEFDVEIKSRRIGRSGALVAATARVIRDSGRADQILVSSFD
                     PIALLQLHCYMPDIALAYLFHDEQPLPLRRGWVGRWIGSSLVHPQNTLCTADRVKAWH
                     TAGMPINAWTVDDPGELERLAALGIDGVFANDPAHAIAVLSAVR"
     gene            complement(2258..>2556)
                     /locus_tag="VF516_43690"
     CDS             complement(2258..>2556)
                     /locus_tag="VF516_43690"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8114734.1"
                     /translation="LDAGPGTFLRQLEVAPRMAGNRFVGWQLIQLLDRSGPLHDVDLT
                     PGDVLLAVNGKPVARPEQLQALWDSLRTANEVTAQLRRGDRPLELRFTVQPPVP"
BASE COUNT          365 a          894 c          951 g          346 t
ORIGIN      
        1 cacccagagc tggtcgatgg cctggtcgcg atcggtctcg tcgcgcagct cgacctgggc
       61 gtcgggccac gcggggtgga acacggggcc ggcgtggccg cagcctccga gcccggcggt
      121 gcccccggcg agtgcgatcg atacggcgat ccggaggctc atacgaacaa gctagcatcg
      181 tcgtggcgtt gagatgaggt atggtgcgcg acgtgactcc gaccggcatc gtggttcaaa
      241 agtacggcgg ctcgtcggtg gccgatgccg acaagatcaa gctggtggcc aagcgcatcg
      301 cgcggacaaa agcggcgggc aagaaggtcg tggtcgtggt atcggcgatg ggcaagacca
      361 ccaaccagct gatcgagaag gcgcggtcga tcagcgagtc gccttcgcgc cgcgagctcg
      421 acatgctgct gacctgcggc gagcgcgagg cgatggccct gctgtcgatg gcgtgctccg
      481 aggaggggct cgaggcgatc tcgttcaccg gctcgcaggc cggcatcctg accaacgatc
      541 gccactccgg cgcgcggatc gtcgaggtcc ggccgttccg catcgaggac gagctcgatc
      601 gcggcaaggt cgtgatcgtc gccgggttcc agggcgtgtc gtacaagcgc gagatcacga
      661 cgctgggccg cggcggctcg gacaccaccg cggtcgcgct cgccgctgcg ctgcgcgccg
      721 actactgcga gatctgctcc gacgtcgacg gcgtgtacac cgccgacccg cgggtggtcg
      781 acgccgccag gctgctcgag tcgatcagcc acgacgagat gctcgagctg gccgcgcagg
      841 gcgccaaggt cctgcacgac gcctcggtcg agttcgcccg caagtccggc atcgcgctgt
      901 acgcccgggc gaccggcaag gacggcggcg gcacgcggat cgacttctcg cccgaccgcg
      961 agcgccaggt cgcggcggtc accggccaga gccagctgat ccggatccgg atcgcgggcg
     1021 gcgcgggcgg tgcgggcggc gcggcggtcg agcactgcct gcagatcctc gagaccgcgt
     1081 cgatcccgct gacccacctc gacctcgagg gccaggagct gcgcgcctgg ttcacgatcg
     1141 ccgacgtccc ggactggggc gaggtccgca agcggctcga caacgtgctc ggcgacgcgc
     1201 tggagctcgg cgaggagggc gcggtgacga tggtcggcca cggcatcggc ggcaacccgg
     1261 gcatcatcaa caaggcgcgc gcgaccgcgg tgggcgccgg cgtcccgctc aacgcggtca
     1321 gcgtgtcgcc gctgcgcgtg accctgtggt gcgatcggcc gcacgtcgat cacctggtgc
     1381 gcgcgctgca caggaccttc atcgagtaga gtagggccat cgaggtacgt ccgccgacat
     1441 gcccaaacct ccgctcgccc ggctgctgcc gcacctcggt cggctcggcc gcgccacccg
     1501 ctggcgccgt acccgaggcc gaccgcgggt atgggcgcac cgcggcgact cggcgcacgc
     1561 tccggagaac acgatgctcg cgttcgaccg ggcgcgcgcc gccggcgccg acgggatcga
     1621 gctcgacgtc cggttcgacg gcgacggcaa cgtcgtggtg ttccacgaca gccagctcga
     1681 gcgcctgacc ggccagccgg gacggatgga ggacctgtcg gcgagcgcgc gcgccgcgct
     1741 gcgggtcggc ggcgagccgg tcccgctgct cgccgaggcg ctggccgggt tcgacctcga
     1801 gttcgacgtc gagatcaagt cgcgccggat cgggcggagc ggggcgctgg tcgccgcgac
     1861 cgcgcgggtg atccgcgact cgggacgcgc cgatcagatc ctggtgtcgt cgttcgaccc
     1921 gatcgcgctg ctccagctcc actgttacat gcccgatatc gcgctcgcct acctgttcca
     1981 tgacgagcag ccgctgccgc tgcggcgcgg ctgggtcgga cgctggatcg gctccagcct
     2041 ggtccacccg cagaacacgc tgtgcaccgc ggaccgggtc aaggcgtggc acaccgcggg
     2101 gatgccgatc aacgcgtgga cggtcgacga cccgggcgag ctcgagcggc tggccgcgct
     2161 cggcatcgac ggcgtgttcg ccaacgaccc ggcgcacgcg atcgccgtgc tgtccgcggt
     2221 ccgctgaccg gcgtcgccgg cctatcgcgg aacgcggtca ggggaccggg ggctggacgg
     2281 tgaaccggag ctcgagcggc cggtcgccgc gccgcagctg ggcggtgacc tcgttcgcgg
     2341 tccgcagcga gtcccacagc gcctgcaact gctcgggccg cgccaccggc ttgccgttga
     2401 ccgcgagcag cacgtcgccg ggtgtcaggt cgacgtcgtg cagcgggccg ctgcggtcga
     2461 gcagctggat gagctgccag ccgacgaacc ggttgccggc catgcgcggc gcgacctcga
     2521 gctggcgcag gaacgtcccg gggcccgcgt cgagca
//
LOCUS       DASVOR010002370         4823 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_404935,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002370 DASVOR010000000
VERSION     DASVOR010002370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_404935"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..978)
                     /locus_tag="VF516_43695"
     CDS             complement(<1..978)
                     /locus_tag="VF516_43695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114735.1"
                     /translation="MLAQHHEQGNEPLRAASCYFQSAERAVRAGDLDAAAERAHRALA
                     YGLPDADRAALLGLLSEVSIWRGEWDEVARWGDQALRLAAPGTHPWGIALSAQMAIVL
                     REGRIDPFLEVLRALLTVEPARDAVVDVAASINFGTLLLSTGGQFAVVDRCLQRIHAI
                     VEPVAHREPVARGWMQLSHVAIEPWAREDPWAGLQYAEAARASFLEAHHRQNARLAQV
                     MVGMNLWFLGALEAAERELRGVLQGGPLHGPPAARHLFCFAGTLADRGALDEAHEVAS
                     RMVEAWQAQGIRIQEGQGRRVLADVLYRRGELAAAEREVRTALALLTFPT"
     gene            1124..1375
                     /locus_tag="VF516_43700"
     CDS             1124..1375
                     /locus_tag="VF516_43700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114736.1"
                     /translation="MKDAALVRRRHGLGDVLSDRQRLARRERPRAQTRREVLTVEPLR
                     DQIRLSQERHRMVEVANDVGMVELGEHAGLALEARRVQI"
     gene            complement(1455..4067)
                     /locus_tag="VF516_43705"
     CDS             complement(1455..4067)
                     /locus_tag="VF516_43705"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8114737.1"
                     /translation="MRFTPAHAGGRGFLLALALTAALAAPRLSSADVITIDDGGKPGT
                     TDLPFTCSVNGDRLLWLPTMGFVYRNVEPFALSPGDTIAFDIQMPGAADLGFRPQVDL
                     SLAHASDPANPFKPDDLTGSDFTIVAHGAVASGAGNQTVRDYDLAFTVDAPFRFPGGG
                     LIIRVSNPQGALATRTDQQCVSVITADTGPTGTNRLVGTFQLEPGEFPFTNVGMGVPG
                     VPYVRITWTARCGDGIVSGKEQCDDGNTDATDRCTNTCTIARCGDGVKAANEECDDGN
                     TDDTDGCTNRCTIAACGDGVKAVDEECDNSASPDHPDPYCDSDCHLHAFAKGSGCSTG
                     GGVGLAVVLVIAALALRRRATGLAILVVGLWAGSAEAQAPRTADGFRVDRFEMAPSVD
                     DGLVVQDPGVLRHMVWSVNAALGYTNTLLRVVPRLSSNDGIDVVGTRLSAYLDFALGL
                     RDRFELHAAVPFAIAQSSESGIAAGLMLRSAGASAVGDGHLGGSVLLYSGSNGARGVQ
                     LGAAADLAIPVGSENSFTSDGKVGGELVATAAYAGPGYRLAANAGVRYRPEINYVSSD
                     QGTELIGRAGLFVPVAHERLMTSLELDTIARAAGHDAFRALGSPILALLGARYHFAGG
                     IRAGAGIGAGLTQAPGSPTVRVIFTVGYSPEPPRTRLAPRPNDRDGDGIIDNLDKCPD
                     QPEDFNGYQDDDGCPDVLETERDRIVDNPPAPGEPLTLEQVITLPAPIEFKFDTAIML
                     PGAEIYLNQVLAILKAHPEVTKLEIQGHTSSEGGYDYNLRLSDDRAKAVYQWLVDHGI
                     DGQRLVPKGYGLTVPLFLNDSEAHRQRNRRVQFRLIEQAPGSAPIHSLPRGRPEALPG
                     TPAPTPTPATPRAP"
     gene            4389..>4823
                     /locus_tag="VF516_43710"
     CDS             4389..>4823
                     /locus_tag="VF516_43710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEX8114738.1"
                     /translation="MTAIAPALFILIWSTGFIVAKFVARVADPNLFLAVRCALAGLLF
                     LVIAVAARARWPHRREIPKHLLAGMLLQGGYLGGTYWAVGQGLAPGVMALLGALQPLL
                     TAVLAIAVLREIPSRRTWVGLLLGVGGVALVLMPGVATRGSGG"
BASE COUNT          660 a         1735 c         1738 g          690 t
ORIGIN      
        1 cgtggggaac gtcaggagcg cgagggcggt gcgcacctcg cgctcggcag cggcgagctc
       61 gccgcgccgg tagagcacgt cggcgaggac gcgacggccc tgcccctcct ggatccggat
      121 cccctgcgcc tgccacgctt cgaccatccg gctcgccacc tcgtgcgcct cgtcgagcgc
      181 cccccggtcg gccagcgtcc ccgcgaagca gaacagatga cgagcggcgg gagggccatg
      241 gagcggcccg ccttgcagga ccccgcgcag ctcgcgctcg gcggcttcca gcgcgccgag
      301 gaaccacagg ttcatgccca ccatcacctg cgcgagccgc gcgttctgcc ggtgatgggc
      361 ctcgagaaag ctcgcccggg ccgcctcggc gtactggagc ccggcccacg gatcctcgcg
      421 ggcccagggc tcgatggcca cgtggctgag ctgcatccag ccccgcgcga ccggctcgcg
      481 gtgcgcgacc ggctcgacga tcgcgtggat ccgctgcagg cagcggtcga caacggcaaa
      541 ctggcccccc gtgctgagca gcaaggtccc gaagttgatg gacgctgcga cgtccacgac
      601 ggcgtcccgc gcgggctcga cggtgagcaa cgcgcggagg acctcgagga aggggtcgat
      661 cctgccctcc cgcagcacga tggccatctg cgcggacagc gcaatgcccc aggggtgagt
      721 gcccggcgcc gccaggcgca gcgcctgatc gccccagcga gccacctcgt cccactcgcc
      781 gcgccagatc gagacctcgg agagcaggcc gagcaacgcg gcccgatcgg cgtccggcag
      841 gccatacgcg agcgcccggt gcgcgcgctc ggcggcggcg tccagatcac ccgcgcggac
      901 cgcgcgctcg gcggactgga aatagcagct cgccgcccgg agcggctcgt tgccctgctc
      961 gtggtgctgg gccagcacca gcggatcgct ctcgccctgg tgctccagcc actccgccgc
     1021 caggcgatgc ccgaggaccc gatcggtcga tcccgaagtc gagcaccttg ctcggaccga
     1081 actccccgcg gatcagaaac acgttgccgg gcctgagatc gcggtgaagg atgccgcgct
     1141 cgtgcgccgc cgccatggcc tcggcgacgt cctgtccgat cgccagcgtc tcgcgcgccg
     1201 tgagcggccc cgtgcgcaga cgcgtcgcga ggtcctcacc gtcgagccac tccgtgacca
     1261 gatacggctg tcccaggagc gtcaccgcat ggtcgaggta gcgaacgatg ttgggatggt
     1321 cgagctcggc gagcatgcgg gcctcgcgct cgaagctcgc agagtccaga tctgaatctg
     1381 tgactttctc ccggtcgctc gtcagacgcg caatctttgc gcaagctgcg gactccggat
     1441 gcgaagtcgc aggatcacgg agcgcgcggc gtcgccggcg tcggcgtcgg cgcgggtgtc
     1501 cccggcaggg cctcgggcct gccgcgcggc agcgagtgga tcggagccga gcccggcgcc
     1561 tgctcgatca gccggaactg cacgcggcga ttgcgctggc ggtgcgcctc gctgtcgttg
     1621 aggaacagcg gcacggtcag accgtagccc ttcggcacga ggcgctggcc gtcgatcccg
     1681 tggtcgacca gccactggta caccgccttg gcccgatcat cggacagccg caggttgtag
     1741 tcgtagccgc cctccgagct ggtgtgaccc tggatctcga gcttggtgac ctcggggtgc
     1801 gccttgagga tggcgagcac ctggttcagg tagatctcgg cgcccggcag catgatcgcg
     1861 gtgtcgaact tgaactcgat cggcgccggc agggtgatca cctgctcgag cgtcagcggc
     1921 tcgccggggg cgggcgggtt gtcgacgatc cggtcgcgct cggtctcgag cacgtccggg
     1981 cagccgtcgt cgtcctggta gccgttgaag tcctccggct ggtccgggca cttgtcgagg
     2041 ttgtcgatga tgccgtcgcc gtcgcggtcg ttgggccgcg gcgccaggcg cgtcctgggc
     2101 ggctcgggcg agtagccgac cgtgaagatc acccgcaccg tcgggctgcc cggcgcctgg
     2161 gtcagccccg cgccgatccc ggcgccagcg cggatcccgc cggcgaagtg gtagcgggcg
     2221 ccgagcaacg ccaggatcgg cgagcccagc gcccggaacg cgtcgtgccc ggccgcgcgc
     2281 gcgatcgtgt cgagctcgag cgacgtcatc aggcgctcgt gggcgaccgg cacgaacagg
     2341 ccggcgcggc cgatcagctc ggtgccctga tcgctgctga catagttgat ctcgggccgg
     2401 tagcgcacgc cggcgttggc cgccagccgg tagcccgggc cggcgtaggc cgccgtcgcc
     2461 accagctcgc cgccgacctt gccgtcgctg gtgaagctgt tctccgagcc gaccgggatc
     2521 gcgaggtcgg ccgccgcgcc gagctgcacc ccgcgcgcgc cgttgctccc gctgtacagc
     2581 agcaccgagc cgccgaggtg cccgtcgccg accgcgctcg cccccgcgct gcgcagcatc
     2641 agcccggccg cgatgcccga ctcgctcgac tgcgcgatcg cgaacggcac cgccgcgtgg
     2701 agctcgaacc ggtcgcgcag gccgagcgcg aagtcgaggt acgccgacag ccgggtcccg
     2761 acgacgtcga tcccgtcgtt ggagctcagc cgcggcacca cgcggagcag cgtgttggtg
     2821 tagcccagcg cggcgttcac gctccacacc atgtggcgga gcacgccggg gtcctggacc
     2881 accaggccat cgtcgaccga cggcgccatc tcgaagcgat cgacgcggaa gccgtccgcc
     2941 gtcctgggcg cctgggcctc ggccgagccc gcccacagcc cgacgaccag gatcgccagg
     3001 ccggtggcgc ggcggcgcag cgccagcgcg gcgatcacca gcacgaccgc gagcccgacg
     3061 ccgccgccgg tgctgcagcc cgagcccttg gcgaacgcgt ggaggtgaca gtcgctgtcg
     3121 cagtacggat cggggtggtc gggggacgcg gagttgtcgc actcctcgtc caccgccttc
     3181 acgccatcgc cgcacgccgc gatcgtgcag cggttggtgc agccgtcggt gtcgtcggtg
     3241 ttgccgtcgt cgcactcctc gttcgccgcc ttcacgccgt cgccgcaccg cgcgatcgtg
     3301 caggtgttgg tgcagcggtc ggtggcgtcg gtgttgccgt cgtcgcactg ctccttgccc
     3361 gacacgatgc cgtcgccgca ccgggcggtc caggtgatcc gcacataggg cacgccgggc
     3421 acgcccatgc ccacgttggt gaacgggaac tcgccgggct cgagctggaa cgtcccgacc
     3481 agccggttcg tgccggtcgg accggtgtcg gcggtgatca ccgagacgca ctgctggtcg
     3541 gtccgggtcg ccagcgcacc ctgcgggttg gacacgcgga tgatcaggcc gccgcctggg
     3601 aaccggaacg gcgcgtcgac ggtgaacgcc aggtcgtagt cgcgaacggt ctggttgccg
     3661 gcgcccgatg ccaccgcgcc gtgggcgacg atcgtgaagt cgctgccggt gagatcatcg
     3721 ggcttgaacg ggttggccgg atccgacgcg tgggccagcg agagatcgac ctggggccgg
     3781 aacccgaggt cggccgcgcc gggcatctgg atgtcgaacg cgatggtatc gccgggcgac
     3841 agcgcgaacg gctcgacgtt gcggtagacg aaccccatcg tcggcagcca cagcagccgg
     3901 tcgccgttga ccgagcaggt gaacggtaga tcggtcgtgc caggcttccc gccgtcgtcg
     3961 atcgtgatga catccgccga ggacaggcgc ggcgccgcca gcgcggccgt gagcgccagc
     4021 gccagcagaa agcctcgtcc accggcgtgc gcgggggtga atcgcatccg taaagtagtg
     4081 aggtcgttgc gtgggcgcgc aagccgcaat gcgtgaggca gacgcgaggg ggtgtgcata
     4141 cgctatcacc gaaagcgagt tcacatctct ccgaggcaga tctcgcccct gacatgtagg
     4201 ccgcacggga tggccaccga cgacaggtag gggtcttcgg aagcggaaca gcgcgtgggc
     4261 gcgcgcctgc gcagtgagct gtggcaaaca ccgctgatgc agcttgctcg atgcgccggc
     4321 cgatcgcgat ccccgcgcag cccgcgcctg ccggtaccat ccggggccgc gttgccgcgg
     4381 atccccactt gaccgcgatc gcccccgcgc tgttcatcct gatctggtcc accggcttca
     4441 tcgtcgccaa gttcgtggcg agggtcgccg atcccaacct gttcctcgcc gtgcggtgcg
     4501 ccctcgcggg cctgctgttc ctcgtgatcg ccgtggcggc gcgggcgcgc tggccgcacc
     4561 ggcgcgagat ccccaagcac ctgctcgccg gcatgctgct gcagggcggc tacctcggcg
     4621 ggacgtactg ggccgtcggc cagggcctgg cgcccggcgt gatggcgctg ctcggcgccc
     4681 tgcagccgct gctgaccgcc gtgctggcga tcgccgtgct gcgcgagatc ccgtcgcgcc
     4741 ggacctgggt ggggctgctg ctcggcgtcg gcggggtcgc cctcgtgctg atgcccggcg
     4801 tcgcgacccg cggcagcggc ggg
//
LOCUS       DASVOR010002371         2534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_346751,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002371 DASVOR010000000
VERSION     DASVOR010002371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346751"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..619
                     /locus_tag="VF516_43715"
     CDS             95..619
                     /locus_tag="VF516_43715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114739.1"
                     /translation="MIQLGWTLLAAAWLGQFLLVGGAAAMLSEPPSQLRAFARGLVQL
                     VRAIVPCLVAALAVAIGSLALVVPGLMLLVLLSLTGASRERGLPAPLLDSLAAARRQL
                     PAVALAVFALFALDAAIGLVAYRAFLAPLPAKPTPIQLAAIRNFVRAIALALVVLSPL
                     PATVLATLRARAQT"
     gene            complement(812..1525)
                     /locus_tag="VF516_43720"
     CDS             complement(812..1525)
                     /locus_tag="VF516_43720"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEX8114740.1"
                     /translation="MQPATGALPIEPPTVTFDAGQTVIDLDLAFLARRLAERGVAVQI
                     PALAAAAPAAWKHYDALVGPSGSHPWRELMTALLAGAGIAADRVGPLVDWLWSEQPRA
                     NLWRAQIPGMVALARALRGRGVRVAILSNSEGRLAELLAEIGIADAFAAIIDSGRVGI
                     EKPDRRIFDHALAVLGATRPGIHIGDSWPADIAGALGAGWRAIWFGRRAVPVDDPRVA
                     VAPDAAEVRAVLDRWLGNL"
     gene            1543..>2534
                     /locus_tag="VF516_43725"
     CDS             1543..>2534
                     /locus_tag="VF516_43725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8114741.1"
                     /translation="MVDGQSAATPVPGAVVIWSGAAPTIQAFRIPADGLVLGRELLTT
                     MTDDRISRQHARVTWRDRRFVVTDLGSRNGTYTGGNPLVDREVTVTAPSVVRTGRTVS
                     VLLEDIRRFEGAAITSARDAIVGPSTAPLWHAVEQAALAGDNLVILGEPGTGKGQMAR
                     GYARARNRQEAVFNPLIQAVPLERVVGAATQTLILEQVGKLAAQDVATLGRLVARPEL
                     RFVTTAVTQLDHLGLPAELAAALSTRTVAVPPLRDRPDEMAFLVHDAVRGAEPGLQIH
                     STLVEVCLLRPWPGNVRELIAEVGRTAHTVAAQGKNNVRGEDLDNDAGHLMAGAP"
BASE COUNT          317 a          938 c          937 g          342 t
ORIGIN      
        1 gccgctgacg ctgctatcgg cgatcgcgct gtcgccgctg gtcgcgctcg ccttccgggc
       61 ccgcgcgccc acggacccgg ccgccgcccg gcccgtgatc cagctcggct ggacgctgct
      121 cgcggccgcc tggctcggcc agttcctcct cgttggcggt gccgccgcga tgctgtccga
      181 gccaccgtcg cagcttcgcg cgttcgcgcg tggcctggtg cagctggtcc gcgcgatcgt
      241 gccctgcctg gtggccgccc tcgcggtggc gatcggcagc ctggcgctgg tggtacccgg
      301 cctcatgctg ctcgtgctgc tgtcactgac gggcgccagc cgcgagcgcg gcctgccggc
      361 gccgctgctc gacagcctcg ccgccgcgcg ccgccagctt cctgcggtcg ccctcgccgt
      421 cttcgcgctg ttcgcgctcg atgcggcgat cggcttggtc gcctaccgcg cattcctcgc
      481 gccgctgcct gccaagccga cgccgatcca gctcgcggcg atccgcaact tcgtgcgcgc
      541 gatcgcgctg gccctcgttg ttctgtctcc gctgccagcc actgtgctcg cgaccctgcg
      601 cgcacgcgcc caaacgtaag gttaaacctc acggttcaga aggtttatcc ttccgatgcg
      661 agcaagggac cgcggcaacg gcgaatggtc catctccaac gcgagcgcgg ggagccgttg
      721 gggggtgctg cgagcggtag agagcgcgag acgccaacgc taggtcgccg tgggcgacct
      781 agcgtgtacg cgttctcccg ggcgctatgc gctacaggtt ccccagccag cggtcgagca
      841 cggcgcggac ctcggcggcg tcaggcgcga ccgcgacccg cgggtcgtcg accggcacgg
      901 cgcgccggcc gaaccagatc gcgcgccagc ccgcgccgag cgcgccggcg atgtcggccg
      961 gccacgagtc gccgatgtgg atgcccggcc gggtggcgcc gagcacggcg agcgcgtggt
     1021 cgaagatccg gcgatcgggc ttctcgatgc cgacccggcc ggagtcgatg atcgcggcga
     1081 acgcgtccgc gatgccgatc tcggcgagca gctcggcgag ccggccctcc gagttggaca
     1141 ggatcgcgac gcgcacgccg cgccccctca gcgcgcgcgc cagcgcgacc atccccggga
     1201 tctgagcgcg ccacaggttg gcccgcggct gctcggacca cagccagtcg acgagcgggc
     1261 cgacccggtc ggcggcgatc ccggcgccgg cgagcagcgc ggtcatcagc tcgcgccacg
     1321 ggtgactgcc ggatggaccg accagcgcgt cgtagtgctt ccaggcggca ggggcggcgg
     1381 cggcgagcgc cgggatctgg acggcgacgc cgcgctcggc gagccggcgg gcgaggaacg
     1441 ccaggtccag gtcgatcacg gtctggccgg cgtcgaaggt gacggtgggg ggctcgatcg
     1501 gaagagctcc ggtggcgggt tgcatgaggg tatactgggc cgatggtcga tgggcagtcc
     1561 gccgccaccc cggttcccgg cgcggtggtg atctggagcg gggcggcgcc gacgatccag
     1621 gcgttccgga tcccggccga cgggctggtg ctcggccgcg agctgctcac caccatgacc
     1681 gacgaccgga tcagccgaca gcacgcccgg gtgacgtggc gcgaccggcg gttcgtggtc
     1741 accgacctgg gcagccggaa cgggacgtac accggcggca acccgctggt cgaccgcgag
     1801 gtcacggtga ccgcgccgtc ggtcgtgcgc accgggcgca cggtgtcggt gctgctcgaa
     1861 gacatccggc ggttcgaggg cgcggcgatc accagcgcgc gcgacgcgat cgtcgggccg
     1921 agcacggcgc cgctgtggca cgcggtcgaa caggccgcgc tggccggcga caacctggtg
     1981 atcctcggcg agcccggcac cggcaagggc cagatggcgc gcggctatgc ccgggcgcgc
     2041 aaccgccagg aagccgtgtt caacccgctg atccaggcgg tcccgctcga gcgcgtggtc
     2101 ggcgcggcga cccagacgct gatcctcgag caggtcggca agctcgccgc ccaggacgtc
     2161 gcgacgctcg gccggctggt cgcgcgcccc gagctccggt tcgtcaccac cgcggtcacc
     2221 cagctcgacc acctcgggtt gccggccgag ctcgccgccg cgctgtcgac ccgcacggtc
     2281 gcggtgccgc cgctgcgcga tcggcccgac gagatggcgt tcctggtcca cgacgccgtg
     2341 cgcggcgccg agcccgggct gcagatccac tcgacgctgg tcgaggtctg cctgctccgg
     2401 ccgtggcccg gcaacgtgcg cgagctgatc gccgaggtcg gtcgcacggc gcacaccgtt
     2461 gcggcgcagg gcaagaacaa cgttcgcggc gaggatctcg acaacgacgc cggccacctg
     2521 atggccggcg cgcc
//
LOCUS       DASVOR010002372         6227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_142770,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002372 DASVOR010000000
VERSION     DASVOR010002372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6227
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142770"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="VF516_43730"
     CDS             <1..425
                     /locus_tag="VF516_43730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114742.1"
                     /translation="AELRRALGVGEAEPIRYRRVQLVCGRHVLSEADNWYVPSRLSAE
                     MNRQLDTTDIAFGRAVQALHFQRRTLSAQLLWSPLPDGWDTAGGALPDASAKVLAIPH
                     AVLEHRAVLTLPDGTPISEVIETYTEQVLAFPQPRPIH"
     gene            1107..4298
                     /locus_tag="VF516_43735"
     CDS             1107..4298
                     /locus_tag="VF516_43735"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114743.1"
                     /translation="MAQDSQRTGESVDSATGIDARDRDVVRPPIRNPDRYHIMGEHGR
                     GGLGRVARAHDRELLRDVAIKELISRNEPDELRFLREALITARLEHPGIVPVHEYGRW
                     PDGTPFYAMKLVSGRSLRDLIAERPTVDQRIGLLHHVIAVADAIAYAHGRNIIHRDLK
                     PGNVIVGDFGETIVIDWGLAKDLKAPDDSSSSGTAAGSVSGSTTGSSGSPPDRAAGDE
                     LTVAGSVLGTPAYMAPEQRRGEHVDQRADVFAIGAMLWELCSPRKTLPANPAQRHRML
                     RRSGIDRDLVAIIDKALDPDPAGRYRDAGALAADLKAFKSGARVGARSYSLYAMLAHW
                     TRRHRAAALSVTTAIAVAATGTVMYVRNIAAERDRADTALHDARRERDHARLSEAALL
                     LENDPNRAKNLLDKLELRSQQYALLTSRMQQLSAAKVVPVSGVVDALFRAPGSATVEL
                     ATRDGGFHRLEPSSAQIETVDHDLAGPVAYRAGQWLYARRTFGAGSVRIASPAQSNLF
                     DVDLPSVSRLVALNDAVYALDGAGDLHRFDTKTATVVDHHVRNIVGDGEVRLACKASG
                     ELEIVRKDVVVDRDRCPPTASPFAMAAVHDDYAALTADHALIASRNGRQLRIPTAIKG
                     EYELAMSSAGVIAISDYRPPGTSWYLRSDGNALEAGPAHSSPPSSVAVDGTLAAWGYG
                     DGTVIVVDTANNTVWPLRGQSGPVNAIVIDAANARVVTAGTRDLRVWELKQPAGVLIK
                     AMPCLIAHAQASPDGRHVALDCKDGNVRVWTRDTATITPVHAHTGSAFGVQWVKGMIC
                     SGGFIDGHVVCSNPDGTDLRMLDSGTNRITWLAASPDHDFLVFASGDGKVWRYDDRLQ
                     ELYQHSSVYRLAISPDGRLLGSCGFDGTLAVYDLANHKLISHVHDHVGQATHVAWVDD
                     ELWTFGIDGAMKRWAVGKTSLSLRHTLDAGGAMHKVKTVHGRWAADAGESVLVIGRDG
                     DSTALSLDLGRVIDALDVSPDQRYVAAGVNGEIVVVDLQRNAVATMTVGSPRPKSVSF
                     LDAVTLAFSEAAALKTVEVDHLEYVTFQPTTEL"
     gene            4664..4840
                     /locus_tag="VF516_43740"
     CDS             4664..4840
                     /locus_tag="VF516_43740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114744.1"
                     /translation="MKKKIRQRTSPESTRQRRLELSRETVRTLGENDLARAVGGSVCD
                     TTSWTTDRTITSHG"
     gene            4927..5103
                     /locus_tag="VF516_43745"
     CDS             4927..5103
                     /locus_tag="VF516_43745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114745.1"
                     /translation="MKKMIRSSITHPERNERRALRLTQETVRLLRPEALAQAMSGCDT
                     TSWTTDAPVASKAC"
     gene            5331..5516
                     /locus_tag="VF516_43750"
     CDS             5331..5516
                     /locus_tag="VF516_43750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114746.1"
                     /translation="MKKNTRGRSANREPKKQRVLRLAQETVRVLNPADLARAAGGSGC
                     DTTSWTTDTRTTATLGG"
     gene            5952..>6227
                     /locus_tag="VF516_43755"
     CDS             5952..>6227
                     /locus_tag="VF516_43755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114747.1"
                     /translation="MRPARASAALAALVFVLTGVLGMVHEATTAHVRCAAHGELVDSD
                     GLAAAVVGPACGTIASAPVLARGHGDEHCLLASAARASRIAPRPPTLI"
BASE COUNT         1049 a         2089 c         2178 g          911 t
ORIGIN      
        1 ccgcggagct gcgccgcgcg ctcggggtcg gcgaggccga gccgatccgc taccggcggg
       61 tccagctcgt gtgcggccgc cacgtgctgt cggaggccga caactggtac gtgccgtccc
      121 ggctgtctgc cgagatgaat cgtcagctcg acaccaccga catcgcgttc ggccgcgcgg
      181 tgcaggcgct gcacttccag cgccggacgc tgtcggcgca gctcctgtgg tctccgctgc
      241 ccgatggctg ggatactgcg ggcggcgcgc tccccgatgc gagcgcgaag gtcctggcga
      301 tcccgcacgc ggtgctggaa caccgggcgg tgctgacgct gcccgacggc acgccgatca
      361 gcgaggtgat cgagacctat accgagcagg tgctcgcgtt cccgcagccc cggccgatcc
      421 actgagccgg cgagtccccg agccggcgat ctccgtacag gcgcagccgg cagcatgccg
      481 gcaagccgat cgacaagagc cgtcgaacga atgttcgagg gctcctgagc gcaacgctct
      541 cggcgcacgg tgtggaatcg cgctgcgagc tctgcgcgag agcggtttcc ataccacctg
      601 gacccttcgc ggtcctccgc agcctcagga atttgaggcg ccggcgtgcc gtcggcgctc
      661 gccccatcta ggagatccat cgctgcgaca ggggcgcgac cacgcgtagg tctcattccg
      721 gatcgggatc aacagatcgg tcagatccca gttttttgac gagcgcaagg tggaaattga
      781 cgcgcgggtg acgtgccgat gtcgggaacc tgcggggcga cccgccccgc acgcgaattt
      841 ccgtcgaaga tcagtgacct agcaacgaac gcccggctcg cgatcggcct ccaccgggtg
      901 tggtcggatc tggggcagcc ccagggtttg tcgtgggtgt ggacaccgtc tgacaccacg
      961 tggaatagcg gagttagcgg atctcagacc ggctcactcc gagctcccgt tgagtgtgga
     1021 cggataggtt tatgtttacg cttacggtga aagtcggagg gaccagcgac tgttcggaga
     1081 cgcagtctgt cgcgccgcca tccggcttgg cgcaggacag ccagcgtacg ggcgagagcg
     1141 tcgactccgc gaccgggatc gatgctcgcg atcgcgatgt cgtccgtcca ccgatccgca
     1201 atcccgaccg ctaccacatc atgggcgagc atgggcgcgg cggcctcggt cgggtcgcgc
     1261 gcgcccacga ccgcgagctc ttacgcgatg tggcgatcaa ggagctgatc tcccgcaacg
     1321 agccggacga gctccggttc ctgcgcgagg cgctgatcac cgcgcggctg gagcaccccg
     1381 ggatcgtccc agttcacgag tacgggcgct ggcccgacgg aacgccgttc tacgcgatga
     1441 agctggtgtc gggtcgctcg ctgcgcgacc tgatcgccga gcggcccacc gtcgatcagc
     1501 ggatcggcct gctccaccac gtgatcgcgg tcgccgacgc gatcgcgtac gcgcacggcc
     1561 gcaacatcat tcaccgcgat ctcaagcccg gcaacgtgat cgtcggcgac ttcggcgaga
     1621 ccatcgtgat cgactggggg ctggccaagg acctcaaggc ccccgacgac tcgagcagca
     1681 gcggaacggc ggccggctcg gtgagcggtt ccacgaccgg ctcgagcggc tcgccgccgg
     1741 atcgcgcggc cggggacgag ctcaccgtgg ccggcagcgt gctcggcacg ccggcctaca
     1801 tggcgcccga gcaacggcgc ggcgagcacg tcgaccagcg cgccgacgtg ttcgcgatcg
     1861 gcgcgatgct gtgggagctg tgctcgccgc gcaagacgct gccggccaac ccggcgcagc
     1921 gccaccgcat gctgcgccgc tccgggatcg atcgcgacct ggtcgcgatc atcgacaagg
     1981 cgctcgatcc ggacccggcg gggcgctacc gggacgccgg cgcgctggcc gccgatctca
     2041 aggcgttcaa gtccggtgcg cgggtcggcg cgcgcagcta ctcgctgtac gcgatgctcg
     2101 cgcactggac ccgccggcac cgcgcggccg cgctgtcggt gaccaccgcg atcgcggtcg
     2161 ccgcgaccgg caccgtcatg tacgtccgca acatcgccgc ggagcgcgat cgcgccgata
     2221 ccgcgctgca cgacgcgcgc cgcgagcgcg accacgcgcg gctgtccgag gcggcgctgc
     2281 tgctggagaa tgacccgaac cgggccaaga acctgctcga caagctggag ctgcgctcgc
     2341 agcagtacgc gctgctgacc agccggatgc agcagctgtc ggcggccaag gtcgtcccgg
     2401 tgtccggagt cgtcgacgcg ctgttccgcg cgcccggcag cgcgacggtc gagctggcga
     2461 cccgcgacgg agggttccat cgcctcgagc cgagcagcgc ccagatcgag accgtcgatc
     2521 acgacctggc gggcccggtg gcctatcggg ccgggcagtg gctgtacgcc cggcggacgt
     2581 tcggcgcggg ctcggtccgg atcgcgagcc cggcgcagtc caacctgttc gacgtcgacc
     2641 tccccagcgt gtcgcggctg gtcgcgctca acgacgcggt ctacgcgctc gacggcgcgg
     2701 gcgatctgca ccggttcgac accaagaccg cgaccgtggt cgaccaccac gtgcgcaaca
     2761 tcgtcggcga tggcgaggtg cgactggcgt gcaaggcgtc cggcgagctc gagatcgtgc
     2821 gcaaggacgt ggtggtggat cgcgaccgct gcccgccgac cgccagcccg ttcgcgatgg
     2881 cggcggtcca tgacgactac gccgcgctga ccgcggacca tgcgctgatc gcgtcccgca
     2941 acggcaggca gctccggatc ccgacggcga tcaagggcga gtacgagctc gcgatgtcga
     3001 gcgccggcgt gatcgcgatc agcgactacc ggccgccggg gacgtcgtgg tacctcaggt
     3061 ccgacggcaa cgcgctggag gccgggcccg cccacagctc cccgccatcc agcgtcgcgg
     3121 tcgacggcac cctcgctgcg tggggctacg gcgacggcac ggtgatcgtg gtcgacaccg
     3181 cgaacaacac ggtctggccg ctgcgcgggc aatcggggcc ggtcaacgcg atcgtgatcg
     3241 acgcggccaa cgcgcgggtc gtgaccgcgg gcacgcgcga tctccgagta tgggagctca
     3301 agcagccggc cggggtgctg atcaaggcga tgccgtgtct gatcgcccat gcccaggcgt
     3361 cgccggacgg ccggcacgtg gcgctcgact gcaaggacgg caacgtgcgg gtgtggacgc
     3421 gcgacaccgc aacgatcacc ccggtgcacg cgcacaccgg atctgcgttc ggcgtgcagt
     3481 gggtcaaggg gatgatctgc tcgggcggct tcatcgacgg acatgtcgtg tgctcgaacc
     3541 ccgatggcac cgacctccgg atgctggact ccggcaccaa ccggatcacg tggctggccg
     3601 cgagccccga ccacgacttc ctggtgttcg cgtccggcga cggcaaggtg tggaggtacg
     3661 acgaccggct gcaggagctg taccagcaca gctcggtata ccgcctggcg atctcgccgg
     3721 atgggcggct gctgggctcg tgtgggttcg acggcacact cgcggtctac gacctcgcca
     3781 atcacaagct gatctcgcac gtccacgatc atgtcgggca ggccacccac gtcgcgtggg
     3841 tcgacgacga gctgtggacg ttcggcatcg acggcgcgat gaagcgctgg gcggtgggta
     3901 agacctcgct gagcctgcgc cacacgctgg acgcgggtgg ggcgatgcac aaggtgaaga
     3961 cggtccacgg ccggtgggcg gcggacgcgg gtgaaagcgt cctggtgatc ggccgggacg
     4021 gtgactcgac cgcgctgagc ctcgacctcg gccgtgtgat cgacgcgctc gacgtgtcgc
     4081 ccgatcagcg ctatgtcgcc gccggcgtca acggcgagat cgtcgtggtc gacctgcagc
     4141 gcaacgcggt cgcgaccatg acggtcggct caccgagacc caagtccgtc agcttcctcg
     4201 acgcggtgac gctcgcattc agcgaggccg ccgcgctcaa gaccgtggaa gtcgatcatc
     4261 tcgaatacgt cacgtttcag cccacgaccg aactctaaaa cagtcagttt cgcggttttc
     4321 gttagttcac tccagcgcta ggagggcctc tcgacgaaca agtgtccgtc gctgtaccac
     4381 ccgtgttgcc aaaatcgtcc ggtagcgact actcacaagg atcggggtta tgaggaaaaa
     4441 aatcaggcga cgtattacga attcggaacc actaagtcag cgcaagttgg ggctgtcacg
     4501 ggagacggtg cgggtgctcg gtgcgaacga tctatcgcag gcgatcgggg gatcgggctg
     4561 cgacacgacg tcgtggacca ccgaccagac gaagacgaaa cagccggggg tctgaggggc
     4621 tcgcgagtca gattcggtag ccatcacaac caggagtgcc tccatgaaga agaagatcag
     4681 gcagcggacg agcccggagt cgacccggca gcgcaggctg gagctgtcgc gggagaccgt
     4741 gcgaacgctc ggcgagaacg atctggcgcg ggcggtcggc ggctcggtct gcgacacgac
     4801 gtcgtggacc acggacagga cgatcaccag tcacggctag ctcgcctgcg agcatcggcg
     4861 gtccgaggga aggtccggcg tttctcgaaa tcgttggtac cacatttttg cagaggggat
     4921 gctgccatga agaaaatgat ccgaagctcc attacacatc cggaacggaa tgaacggcgg
     4981 gcgctgcggc tgacccagga gacggtgcgg ctgctgcgcc ccgaggcgct ggcacaggcg
     5041 atgtcgggct gcgacaccac gtcgtggacg accgacgctc cggtggctag caaggcctgc
     5101 tagcgcgatc ctgggcctgc gagtcctgtt ccgaatcagg aatcgagcat gagcaagtcg
     5161 cgcggtcacg ccggggggcg gtcaggctgc gcgacgacgc gcgggccacg agcgaaacgc
     5221 gcggcggaca cacgctgccg cagaagtaag acgtacaggt gcaagggccg gcgggcttcg
     5281 cagtactccg aaatcgctgg acaactactc atttcacagg ggatacttcc atgaagaaga
     5341 acacgcgagg ccgttctgca aatcgggaac caaagaaaca acgcgtgctg cggctggcgc
     5401 aggagaccgt cagggtgctg aatccggccg acctggcgcg cgcggccggc ggctcgggct
     5461 gcgacacgac gtcgtggacc acggacacgc gcaccaccgc caccctcggt gggtgactcg
     5521 gtgagttcgc tcacagccat gtagctgatt caaatctcag tgaaccacag ggtgcctcca
     5581 tgaagaagac ctcacgtagt attacgcatg cggaatcaat cacgcggcgg aggttgcggt
     5641 tgtcccggga ggcgatccgg acgttgcagg ccggcgacct gtcccgcgtg aacgctggct
     5701 gcgacaccac ctcgtggacg accgacagca cccaggcccc gccagcgacc gctcgctgct
     5761 agtgagaccg gccggccggc gatccattcg atgccgcagc ggcccgggtc gaggcaagcc
     5821 accccaaccc gggggagcca cggcgggcca gcggtttttc cgcaggagat gtggtactcc
     5881 ccgggagacc gaccttgctg tgcctggaac gccagatcac cccgggtgac cggatccgac
     5941 gctcgacgtc ggtgcgtcct gcgcgcgcgt cggcggcgct ggcggcgctg gtattcgtgc
     6001 tcaccggcgt gctcgggatg gtccacgagg ccaccaccgc gcacgtccgg tgcgccgccc
     6061 acggcgagct ggtcgatagc gacgggctcg ccgcggcagt ggtcggaccg gcgtgcggca
     6121 cgatcgcgag cgcaccggtc ctcgcccgcg gccacggcga cgagcactgc ctgctggcgt
     6181 cggcggcgcg cgcgtcgcgg atcgcaccgc ggcccccgac cctgatc
//
LOCUS       DASVOR010002373         2649 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_552687,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002373 DASVOR010000000
VERSION     DASVOR010002373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552687"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1325
                     /locus_tag="VF516_43760"
     CDS             <1..1325
                     /locus_tag="VF516_43760"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8114748.1"
                     /translation="LTGLALASPELRAEFASQGVDEESVRVQAAECALLAADAYQWLD
                     AEHHHWGALLEAASLLQGSAFPERLLQAFQLIALARNNRVLYCEHHRCDDSAADPPPQ
                     LERYPLAEDSDPGRSKRERQLFDDAYRAWLTASGRIQSSDDPGTVVALGYFARYLSGV
                     RQLFGSTWSSGLQIELEADADAATPPRAVDWLIERVRHHYGPLDLERILDFGCGTGDD
                     ARHLARRHQVWAVESPLWFAATRGEPPAAGPAAHFLELDPVEFARRICDGRPLDGAPP
                     PLDVVLFRCSLCRISQRALVLQAAHRLLRPGGLVIATDWVQTRITDRITWSRVVGTGR
                     FVDLETEPGYRRLCRDQRFTDFVSWDWTAEPGVGAPAMHRWFQARLEDVRRLEDERGR
                     PPRSPHERAFLLRLKRDLEALAALSTAEGPLGWLFWAARKPLAGPAGD"
     gene            1871..2536
                     /locus_tag="VF516_43765"
     CDS             1871..2536
                     /locus_tag="VF516_43765"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8114749.1"
                     /translation="MEPLDLLAGLRKYSPSVVHFSGHGTSPTTGAPVHRPGRRDIETS
                     VHGHRLAGLCFQRPDGGVEVVSPGALKDTLRAVGSSVRLVVLNACYSELQAKALRAHV
                     DCVIGTRGTIQDDAAIHFATGLYGGLAGGASIASAFKQGVAALSLKGLADRPQLLVRP
                     GVDASTMVLADRAPDDGFRPRRLTVASRNRPGPRVPGGTRVIRPAASSVRAAVMQRRP
                     VGG"
BASE COUNT          354 a          950 c          942 g          403 t
ORIGIN      
        1 cgctgaccgg gctcgcgctg gcgagccccg agctgcgcgc ggagttcgcg tcgcagggcg
       61 tcgacgagga gagcgtgcgc gtccaggccg ccgagtgcgc gctgctggcc gccgacgcgt
      121 accagtggct cgacgccgag caccaccact ggggcgcgct gctcgaggcg gcctcgctgc
      181 tgcaaggcag cgcgttcccc gagcgcctgc tccaggcgtt ccagctgatc gcgctcgccc
      241 gcaacaaccg cgtgctctac tgcgagcacc accgctgcga cgacagcgcc gcggatccgc
      301 cgccgcagct cgagcgctac ccgctcgccg aggacagcga tcccggccgc agcaagcgcg
      361 agcgccagct gttcgacgac gcctaccggg cgtggctgac cgcctcgggc cgcatccaga
      421 gctcggacga cccgggcacg gtcgtggccc tcggctactt cgcccgctac ctgtccgggg
      481 tccggcagct gttcggatcg acctggtcga gcggcctgca gatcgagctc gaggccgatg
      541 ccgacgccgc gacgccaccc cgcgcggtcg attggctgat cgagcgggtc cgccaccatt
      601 acggtccgct ggacctggag cgcatcctcg acttcgggtg cggcacgggg gacgacgcgc
      661 ggcacctcgc gcgccgccac caggtctggg cggtggaatc gccgctgtgg ttcgccgcga
      721 cgcgcggcga gccgccggcc gccggccccg cggcgcactt cctcgagctc gatccggtcg
      781 agttcgcccg ccggatctgc gacggccggc cgctcgacgg cgccccgccc ccgctcgacg
      841 tcgtgctgtt tcgctgctcg ctgtgccgga tcagccagcg cgcgctggtg ctccaggcgg
      901 cgcaccgcct gctccgcccc ggcggcctcg tgatcgcgac cgactgggtc cagacccgga
      961 tcaccgaccg gatcacctgg tcgcgcgtcg tcggcaccgg gcggttcgtc gacctcgaga
     1021 ccgagcccgg ctaccggcgg ctgtgccgcg atcagcggtt caccgacttc gtcagctggg
     1081 actggacggc cgagcccggc gtcggcgcgc cggcgatgca tcgctggttc caggcccggc
     1141 tcgaggacgt gcgccggctc gaggacgagc gcggccggcc gccgcgctcg ccgcacgagc
     1201 gcgcgttcct gctccgcctc aagcgcgatc tcgaggcgct cgccgcgctg agcaccgccg
     1261 agggtccgct cggctggctg ttctgggccg cccgcaagcc gctcgcgggt ccggccggcg
     1321 actgacctgt accggtgcga cggccggcga cgcgccgcga tcggccgcag ctctcgggcc
     1381 tcggcttgcg atggtctttg tcttgcagat ttgcggactg acgatgtcaa gtccccatga
     1441 tgattgaccc gaggttcgac ggccgtggca ggaacagatc gcgagtcggc ccgagtggga
     1501 taccgttaaa aatcatgccg gtcagcggga atctccctga cagggtgtcg aaaaacgtgg
     1561 atcactgctc gccgcgctgc accctcattc ggaggccggt acagatgaag ttgcgatcga
     1621 gagcacggat ccttggcgtt ctcgccgtgt ccctcgccac atgtgcgacg ggcagtttgg
     1681 cgcggcggat caatcgtggg atgccgcgag gggcgatgtg aagaagtgga agaaacacgt
     1741 gatcttgatg ttcagtgcca gtccatcgca cgggcaccgg ctggcgctcg accgagaagc
     1801 acgggcgctc gagtccgaga tagatcggtc tctctgtcgc gatcagttcg agctccagac
     1861 ctggtgggcc gtggaaccgc tcgatctgct cgccggcctg cgcaagtaca gcccgagcgt
     1921 cgtgcacttc agcggccacg ggacctcgcc gacgaccggc gcgccggtgc accgtccggg
     1981 gcggcgcgac atcgagacgt ccgttcacgg ccatcgcctg gccggcctgt gctttcaacg
     2041 gcctgatggc ggcgtcgagg tcgtgtcgcc gggcgccctg aaggacacgc tgcgcgccgt
     2101 gggatcgtcc gtccggctcg tcgtgctcaa cgcctgctac agcgagctgc aggccaaggc
     2161 cttgcgcgca cacgtcgatt gcgtgatcgg gacgcgcgga acgatccagg acgacgcggc
     2221 gatccacttt gcgaccgggc tgtacggcgg actcgccgga ggagcctcga tcgcgagcgc
     2281 gttcaaacag ggtgtcgcgg cgctgagcct gaaagggctg gccgatcgcc cccagctcct
     2341 ggtgcgacct ggcgtcgatg ccagcaccat ggtcctggct gatcgtgcgc ctgatgacgg
     2401 gttccgcccg cggcgcctga ccgtggcgag ccgaaatcgc cccgggccgc gggtacctgg
     2461 aggaacccgc gtgatccgcc ctgcggcatc gagcgtccgc gcggcagtga tgcagcggcg
     2521 cccggtcggt ggttaactca ccggcgtgct gaccgaggct gccgcgcgct ggctgctcgt
     2581 gctccacacc gggctcgggg tcgcggcggt cggggcggcg acccacctgg tgctgtggct
     2641 gcgccgcct
//
LOCUS       DASVOR010002374         2235 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_84602,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002374 DASVOR010000000
VERSION     DASVOR010002374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84602"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2137)
                     /locus_tag="VF516_43770"
     CDS             complement(<1..2137)
                     /locus_tag="VF516_43770"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEX8114750.1"
                     /translation="MLARFFANRPVFAWVITIAVILAGIASIRALPIAAYPDVALPAV
                     NIAAGYPGASAETVENSVTQVLEQQLTGIDGLVYFSSSSNSSGQATINVTFKQGTNPD
                     TAQVQVQNKVQQAIPRLPPAVQQAGIVVSKAQSNFLMVVIIYDDSDKATDADLADYLT
                     STIQDPIARVDGVGNIQIFGGSYAMRIWLDPTKLQAFSLMPSDVQAAIATQNVQLSAG
                     KIGQQPSAPDQQLNATVTAQSKLRTPQQFRDIILKYDPSGATVRLGDVARVELGSEVY
                     GFAVRHNRHPAGGFAVNLAPNANAMAVASAVKDTVNRLEGTMPAGWKVSYPFDTTKFI
                     RLSILEVVKTLIEAIALVVIVMFVFLQSWRATLIPVIAVPVVLLGTFCILQLLGYTIN
                     TLTMFAMVLSIGLLVDDAIVVVENTERLMREEHLSPLDATVRSMKEITGALIGIATVL
                     SAVFLPMAFFSGSTGVIYRQFSVTIVASMLLSVLVALTISPAMCASLLRSKGEHGASH
                     RGPFGWFNRGLEGTVRGYQGGVARMLRGPFRWLVPYAAIVGLMVWLVFRLPTGFLPNE
                     DQGLGFAVWTLPSGATLPRTVEVGKAVENHFLDVEKASVEAVATVAGFSFAGFAQNTG
                     IAFLELKDWDQRPGKANTTQAVTGRAIAMLSAVRDAQIFALTPPPIQGLGQSQGFDFQ
                     LQAAGIDRAALAQARDQLLAAAAKDPRLIA"
BASE COUNT          338 a          757 c          793 g          347 t
ORIGIN      
        1 ccgcgatcag cctggggtcc ttggccgcgg ccgcgagcag ctggtcccgg gcctgcgcga
       61 gtgcggcgcg gtcgatgccg gcggcctgga gctggaagtc gaagccctgg gactgtccca
      121 ggccctggat cggcggcggg gtcagcgcga agatctgggc gtcgcgcacc gccgacagca
      181 tggcgatcgc ccggccggtg accgcctggg tggtgttggc cttgcccggg cgctggtccc
      241 agtccttgag ctcgaggaat gcgatgccgg tgttctgggc gaagccggcg aagctgaacc
      301 ccgcgaccgt cgcgaccgcc tcgacgctgg ccttctcgac gtcgaggaag tggttctcga
      361 ccgccttgcc gacctcgacg gtgcgcggca gcgtcgcacc cgacggcagc gtccacaccg
      421 cgaacccgag accctggtcc tcgttcggca ggaacccggt cggcagccgg aacaccagcc
      481 acaccatcag gccgacgatc gcggcgtagg gcacgagcca gcggaacggg ccgcgcagca
      541 tccgcgcgac gccgccctgg tagccgcgca ccgtgccctc gaggccgcgg ttgaaccagc
      601 cgaacggccc gcggtggctc gcaccgtgct cgcccttgct gcgcagcagc gacgcgcaca
      661 tcgccggcga gatcgtgagc gcgaccagca ccgacagcag catcgacgcg acgatcgtga
      721 ccgagaactg gcggtagatc acgccggtcg agccgctgaa gaacgccatc ggcaggaaca
      781 ccgccgacag cacggtcgcg atgccgatca gcgcgccggt gatctccttc atcgaccgca
      841 cggtggcgtc gagcggcgac aggtgctcct cgcgcatcag ccgctcggtg ttctcgacca
      901 cgacgatcgc atcgtcgacc agcaggccga tcgacagcac catcgcgaac atggtcagcg
      961 tgttgatcgt gtaaccgagc agctggagga tgcagaacgt gccgagcagc acgaccggca
     1021 ccgcgatcac cgggatcagc gtcgcccgcc agctctgcag gaacacgaac atcacgatca
     1081 cgaccagggc gatcgcctcg atcagcgtct tgacgacctc gaggatcgac agccggatga
     1141 acttggtggt gtcgaacgga tacgagacct tccagcccgc cggcatcgtg ccctcgagcc
     1201 ggttcaccgt gtccttgacc gcgctcgcca ccgccatcgc gttggcgttg ggcgcgaggt
     1261 tcaccgcgaa gccacccgcg gggtggcggt tgtgccgcac cgcgaagccg tagacctcgc
     1321 tgccgagctc gacgcgcgcg acgtcgccga gccgcaccgt cgcgccgctc gggtcgtact
     1381 tgaggatgat gtcgcggaac tgctgcggcg tgcgcagctt ggactgcgcg gtcaccgtgg
     1441 cgttgagctg ctggtcgggg gcactcggct gctggccgat cttgccggcc gagagctgga
     1501 cgttctgggt cgcgatcgcg gcctggacgt ccgacggcat caggctgaat gcctggagct
     1561 tggtcgggtc gagccagatc cgcatcgcgt agctgccgcc gaagatctgg atgttgccga
     1621 cgccgtcgac ccgcgcgatc ggatcctgga tcgtgctcgt gaggtagtcc gcgaggtcgg
     1681 cgtcggtcgc cttgtcgctg tcgtcgtaga tgatcacgac catcaggaag ttgctctgcg
     1741 ccttggacac cacgatgccc gcctgctgca cggccggcgg caggcgcggg atcgcctgct
     1801 ggaccttgtt ctggacctgg acctgcgcgg tgtcggggtt ggtgccctgc ttgaacgtga
     1861 cgttgatggt ggcctggccg gacgagttcg acgagctgga gaagtagacc aggccgtcga
     1921 tcccggtcag ctgctgctcg agcacctggg tcacgctgtt ctcgacggtc tcggccgacg
     1981 cgccggggta gccggcggcg atgttgactg ccggcagcgc gacgtcgggg tacgccgcga
     2041 tcggcagcgc gcggatcgag gcgatgccgg cgaggatcac ggcgatcgtg atcacccacg
     2101 cgaacaccgg tcggttggcg aagaaccggg cgagcacgtc agcgctcctt ggcttcgggt
     2161 ttggccggcg ccgcctccgg cttggccggt gcatcgggcc tggccggcgc atcgggcttg
     2221 gccggcgcac cctcg
//
LOCUS       DASVOR010002375         2329 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_84613,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002375 DASVOR010000000
VERSION     DASVOR010002375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84613"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..108)
                     /locus_tag="VF516_43775"
     CDS             complement(<1..108)
                     /locus_tag="VF516_43775"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEX8114751.1"
                     /translation="MKRLLGKFAIVTGGSSGIGAACAQALAAEGAAVVAT"
     gene            220..1305
                     /locus_tag="VF516_43780"
     CDS             220..1305
                     /locus_tag="VF516_43780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114752.1"
                     /translation="MRRMAGMRTLAALPLFLAGCGDNAHHDPDGGSRDAIAGAECLPA
                     ANCTTDCWCVDPQVGTAALLLGVWGSSPTSVWAVGENGTILHWDGAAWSLQRSGTSAM
                     LSGIHGTGDSDVWAVGGAFDATGVNATTLHWNGSSWSNVPISARVHLQRVWAIAPDDV
                     WAVGDLAGDGSVPNFQQMVQHWDGTQWTVAPGNPNGESVPMFHGVWASSANDVYVAGG
                     FRSLTGFTFVTRWDGTKWTGSRSVDSDIVFGIHGTGPNDIWAGSSGGYPHGFDSWVLH
                     FTGAVASPDAQLLRNHQLSQLWVADSMHIFFVGQYGHIVRGDGTTWTALASGTDTNLW
                     GVWASAANDVWAVGEDGLVLHRAPQRR"
     gene            complement(1306..>2329)
                     /gene="trmFO"
                     /locus_tag="VF516_43785"
     CDS             complement(1306..>2329)
                     /gene="trmFO"
                     /locus_tag="VF516_43785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEX8114753.1"
                     /translation="TGPLTEGALGEVIRAELGGDRMYFYDAIAPIVAADSIDGDFAFR
                     GSRWGRDTEPAEPAERREGDGDRAAGGDVGVGDYVNCPLDRAQYEAFVALVNAGRKVL
                     PHDFEEPRYFESCMPIEVMAERGPETLRFGPMRPIGLRDPRTGHRPWAVVQLRPENRY
                     LTAYNLVGFQTRLAYPEQQRIFQTIPALHRAEFLRFGSIHRNTYIDAPSRLGPHFELV
                     TRPSVRFAGLLTGVEGYIESCAMGLVVAWLLAGELGGRDVPPPPPTTMLGGLYHHVTA
                     PREPGYKYGPTNVNYGLLPPLSGVRKDQKKPRMAARANADLEAWLAALGRAAPAGEPA
                     SAARGS"
BASE COUNT          333 a          827 c          825 g          344 t
ORIGIN      
        1 ggtcgccacc accgccgcgc cctcggccgc cagcgcctgg gcgcacgccg ccccgatgcc
       61 gctggagccg ccggtcacga tcgcgaattt gccgagcaag cgcttcatcg tgcctgcaac
      121 ctaccaccgc ggtcacccgt cattccggag cgagccctgg ttcaggtttg ccggtggagt
      181 ccaccgctca ctcgtcgagg cgcgtgctac gctgaccgcg tgagacggat ggcagggatg
      241 aggacgctcg ccgcgctccc gctgttcctc gcgggctgcg gcgacaatgc ccaccacgac
      301 ccggacggcg gatcgcgcga tgcgatcgcg ggtgcggaat gcctgccggc cgcgaactgc
      361 accaccgatt gctggtgcgt ggacccacag gtcgggaccg ccgcgctcct gctgggtgtg
      421 tggggcagct ccccgacgag cgtctgggcc gtcggcgaga acgggacgat cctgcactgg
      481 gacggcgcgg cctggtcgct gcagcggtcg gggacctcgg cgatgctgtc cggcatccac
      541 ggcaccgggg acagcgacgt gtgggcggtc gggggagcct tcgacgccac cggcgtcaac
      601 gccacgaccc tgcactggaa cggcagctcg tggtcgaacg tgccgatctc cgcccgcgtt
      661 cacctgcagc gcgtgtgggc catcgctccc gatgatgtgt gggcggtcgg cgatctggcc
      721 ggcgatggga gcgtcccaaa cttccaacag atggtgcagc actgggacgg cacgcaatgg
      781 acggtcgcgc ccggcaaccc gaacggggaa tcggtgccga tgttccatgg ggtctgggcg
      841 agcagcgcga acgacgtgta tgtcgccggt ggtttcagat cgctcacagg gttcaccttc
      901 gtcacccgct gggacggtac gaaatggacc ggttcccgca gcgttgactc cgacattgtg
      961 ttcgggatcc acgggaccgg gcccaacgac atctgggcgg gcagctcggg cggctaccct
     1021 cacgggtttg acagctgggt actgcacttc acgggtgcgg tcgcgtcgcc cgacgcccag
     1081 ctgctgcgga accaccagct atcacaactc tgggtcgcag attccatgca catcttcttc
     1141 gtcggtcagt acggccacat cgtgcgcggc gacggcacga cgtggaccgc gctcgcgagc
     1201 ggcacggata ccaatctatg gggcgtgtgg gcaagcgcgg ccaacgatgt ctgggccgtc
     1261 ggcgaagacg gcctggtgct tcaccgcgct ccacagcgga gatgatcacg acccccgcgc
     1321 ggcgctcgcc ggctcacctg ccggcgcggc gcgccccagc gccgccagcc aggcctcgag
     1381 gtcggcgttc gcccgcgcgg ccatccgcgg cttcttctgg tccttgcgca caccggacag
     1441 cggcggcagg aggccgtagt tgacgttggt cgggccgtac ttgtatcccg gctcgcgcgg
     1501 cgcggtgacg tggtggtaca gcccgccgag catcgtcgtc gggggcggcg gcgggacgtc
     1561 gcggccgccg agctcgccgg cgagcagcca cgcgaccacc agccccatcg cgcacgactc
     1621 gatgtagccc tcgaccccgg tcagaaggcc cgcgaaccgc acgctcggcc gggtcacgag
     1681 ctcgaagtgc gggccgagcc ggctcggcgc gtcgatgtac gtgttgcggt ggatcgaccc
     1741 gaagcgcagg aactcggcgc ggtgcagcgc cgggatggtc tggaagatcc gctgctgctc
     1801 ggggtacgcc aggcgggtct ggaagccgac caggttgtag gcggtgaggt agcggttctc
     1861 gggccggagc tggaccaccg cccacggccg gtgcccggtc cgcggatcgc gcagcccgat
     1921 cggccgcatc ggcccgaacc gcagcgtctc gggcccgcgc tcggccatca cctcgatcgg
     1981 catgcagctc tcgaagtagc gcggctcctc gaagtcgtgc ggcaacacct tgcggccggc
     2041 gttgaccagc gcgacgaacg cctcgtactg cgcccggtcg agcgggcagt tgacgtagtc
     2101 gccgacgccg acgtcgccgc cggccgcgcg atcgccgtcg ccctcccgcc gctcggcggg
     2161 ctcggccggc tcggtgtcgc ggccccaccg cgagccgcgg aacgcgaagt ccccgtcgat
     2221 cgagtccgcc gccacgatcg gcgcgatcgc gtcgtagaag tacatccggt cgccaccgag
     2281 ctcggcgcgg atcacctcgc caagcgcgcc ttcggtcagc ggcccagtc
//
LOCUS       DASVOR010002376         3107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_201503,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002376 DASVOR010000000
VERSION     DASVOR010002376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201503"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..304)
                     /locus_tag="VF516_43790"
     CDS             complement(<1..304)
                     /locus_tag="VF516_43790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114754.1"
                     /translation="MADDDRALRERLAALEAEVQADADAQRARKAAALAKLRDQRVTK
                     TAGGAPVVRNRATPGDDAAARRRSRLAERDADDDHRGRGAEALEDLGGALELASKAN"
     gene            333..659
                     /locus_tag="VF516_43795"
     CDS             333..659
                     /locus_tag="VF516_43795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114755.1"
                     /translation="MSFRSISLGFSLGLVAALALGCGGDPPRSDDPQTAKDKQRREAA
                     AAGETSSAGGKWGGWRYTGDRDACFFIVGRKCFKTEASACAAARCGSRKCETTGGGPA
                     TVACAK"
     gene            complement(660..1706)
                     /locus_tag="VF516_43800"
     CDS             complement(660..1706)
                     /locus_tag="VF516_43800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114756.1"
                     /translation="MITNPNSRKNRNRPDRADRLRRIVGDLGEVHTTSSVESIKPVLR
                     EFLRKRARYWVADGGDGALHWMVRMGMEVLQEDEFIGESSIGSPALPMTLPTKGGTID
                     FVANNVGIEGDAEGILATLRRNVERNVQIEETEVDSMAIDGIQVVDGAEVPFRTYGFA
                     SAAGGVGQRFYAKYYDAPDPNPRTIVKVIATAVASAPIALSPLSRLPLGSWASYAKDV
                     FEPTRCRVTLDGMRLPGDQFTGVHIASMSINLGNVLRFFGKADRPGLMNAIVGTPSPW
                     GVIRNLPRMRRGEEMRGRNILDRPCREMTMEAAGDDLLAPIIDGEFYRNVKRLAFHVG
                     PRVRIPKVVAPKAA"
     gene            1985..3022
                     /locus_tag="VF516_43805"
     CDS             1985..3022
                     /locus_tag="VF516_43805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114757.1"
                     /translation="MRPFVLGLCLAACGGATVPASSPPPPAPRRVATEPDRPPVDPIA
                     ALTPDDTAARSWVVPGPIYLELGAAAIAGGGAGRPIEVTPIDEEGNLQRVAVRLDHAR
                     FSVWIDRTQMLAVLTRDQRIDALGVPAIGGEVQVVLRAGATVRRLAHRDRSTQVRYLG
                     ALEIEGWVPDAVLADRSPPRDGTGRIPTGRKTFMVLPGAVIRAEPRWAASELALMGTA
                     YSLDIVRELDDAWTEVSYEDGDLVVHGFVSRRDPPGRLHHWSQSDPPPTVAPNATVAS
                     GTCLYDRTAGDAIGYVVGNRPVALDDLGNGWWSMTPDTPWGALGFAAHGPSKTELAAC
                     APPGTVPPVTP"
BASE COUNT          413 a         1184 c         1044 g          466 t
ORIGIN      
        1 ggttggcctt cgacgcgagc tcgagcgcgc cgccgagatc ctccagcgcc tcggcgccgc
       61 gaccgcggtg gtcgtcgtct gcgtcgcgct ccgccaaccg tgaccggcgt cgcgccgcgg
      121 cgtcgtcgcc cggcgttgcg cggtttcgca caaccggcgc gccgcctgcg gtcttggtca
      181 cacgctggtc gcgcaacttc gcgagcgcgg cggccttgcg cgcgcgctgc gcgtccgcgt
      241 cggcctggac ctcggcctcc agcgcggcca gtcgctccct cagtgcgcgg tcatcgtcag
      301 ccacggcttc gactatggca tgatctgccg cgatgtcgtt ccggtcgatc tcgctcggtt
      361 tctcgctcgg cttggtcgcc gcgctcgcgc tcggctgcgg cggagatccg ccgagatccg
      421 acgatccgca gaccgccaag gacaagcagc gccgcgaggc cgcggcggcc ggcgagacct
      481 cgtccgccgg cggcaagtgg ggcggctggc gctacaccgg agatcgcgac gcgtgcttct
      541 tcatcgtcgg ccgcaagtgc ttcaagaccg aggcatcggc ctgtgcggcc gcgcgctgcg
      601 gcagccggaa gtgcgagacc accggaggcg gccccgccac cgtggcctgc gcgaagtgac
      661 tacgccgcct tgggcgcgac gaccttgggg atccggaccc gcggcccgac gtggaacgcc
      721 agccgcttga cgttgcgata gaactcgccg tcgatgatcg gcgccagcag gtcgtcgccg
      781 gcggcctcca tggtcatctc gcggcacggc cggtcgagga tgttgcggcc gcgcatctcc
      841 tcgccgcgcc gcatccgcgg caggttgcgg atcacccccc acgggctcgg cgtgccgacg
      901 atcgcgttca tcagccccgg gcgatccgcc ttgccgaaga accgcagcac gttgccgagg
      961 ttgatcgaca tcgacgcgat gtggaccccg gtgaactggt cgcccggcag ccgcatgccg
     1021 tcgagcgtga cccggcagcg cgtcggctcg aacacgtcct tggcgtagct cgcccacgag
     1081 ccgagcggca gccgcgacag cggcgacagc gcgatcggcg ccgaggcgac cgcggtcgcg
     1141 atcaccttca cgatcgtccg cgggttggga tccggggcgt cgtagtactt ggcgtagaac
     1201 cgctggccga cgccgccggc cgccgacgcg aagccgtagg tgcggaacgg cacctcggcg
     1261 ccatcgacca cctggatccc gtcgatcgcc atcgagtcga cctcggtctc ctcgatctgg
     1321 acgttgcgct cgacgttgcg gcgcaaggtc gccaggatcc cctcggcgtc gccctcgatg
     1381 ccgacgttgt tggcgacgaa gtcgatcgtg ccgcccttgg tgggcagcgt catgggaagt
     1441 gccggagatc cgatcgatga ctcgccgatg aactcgtcct cctgcaatac ctccatgccc
     1501 atccgcacca tccagtgcag cgcaccatct ccgccatcag ccacccagta gcgcgcccgc
     1561 ttgcgcagga actcgcgcag gaccggcttg atcgactcca ccgagctcgt ggtgtgtacc
     1621 tcgccgagat cacccacgat tcgccgcaac cgatccgccc ggtctggccg gttgcggttc
     1681 ttgcggctgt ttggattggt gatcacgccg atctgcatct ggcccctgcc gtctgcaaga
     1741 gccgggcgag acgcggcgcg tcgcaacttg tcaagatcac gtgacaggaa cgccacgttt
     1801 gtcgctcagt gcgtaccgac ttacgcagcc aagtggatag ttggcgcgcc ggtgacacgc
     1861 tccgtcatca cgtaactgcg caatcccaac acgctgctgc gttccggttc cggtctggcc
     1921 agcgcggtcc aatcgcgaca gtagcggtcg gtccgcgccc agcgcattct tttagaagcc
     1981 cagcatgcgc ccgtttgttc tcggcctctg cctggcagcc tgcggcggtg ccaccgtccc
     2041 ggcgtcctcc cccccgcccc cggcgccccg gcgcgtcgcc accgaaccgg accgcccgcc
     2101 ggtcgacccg atcgccgcgc tcacccccga cgacaccgcg gccaggtcct gggtggtccc
     2161 cggtccgatc tacctcgagc tcggcgcggc ggcgatcgcc ggcggtggcg ccggccggcc
     2221 gatcgaggtc acgccgatcg acgaggaggg caacctccag cgcgtcgccg tccgcctcga
     2281 ccatgcgcgg ttctcggtct ggatcgatcg gacgcagatg ctcgcggtgc tgacccgcga
     2341 ccagcggatc gatgcgctcg gcgtcccggc gatcggcggc gaggtccagg tcgtgctgcg
     2401 cgccggcgcg accgtccgtc gcctcgccca ccgcgatcgc tcgacccagg tccgctacct
     2461 cggcgcgctc gagatcgagg gctgggtccc cgacgcggtg ctcgccgacc gcagcccgcc
     2521 acgcgacggc accggccgga tcccgaccgg gcgcaagacc ttcatggtcc tgcccggcgc
     2581 cgtgatccgc gccgagccgc gctgggccgc cagcgagctg gcgctgatgg gcaccgccta
     2641 ctcgctcgac atcgtccgcg agctcgacga cgcgtggacc gaggtcagct acgaggacgg
     2701 cgacctcgtc gtccacggct tcgtgtcgcg ccgcgatccg cccggccgcc tccaccactg
     2761 gagtcagagc gatccgccgc cgaccgtcgc ccccaacgcc accgtggcca gcggcacctg
     2821 cctctacgac cgcaccgccg gcgacgcgat cggctacgtc gtcggcaacc gcccggtcgc
     2881 gctcgatgac ctcggcaacg gctggtggag catgacgccc gacaccccgt ggggcgcgct
     2941 cgggttcgcc gcccacgggc cgagcaagac cgagctggcg gcctgcgcgc cgcccggcac
     3001 cgtcccgccg gtcacaccgt agcggcccgc ggctacgagc cggtgaacgt cggcgggcgc
     3061 ttggcgagca gcgcggcgat gccctcgcgc gcgtcgctgg tggcgac
//
LOCUS       DASVOR010002377         3470 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_318415,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002377 DASVOR010000000
VERSION     DASVOR010002377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3470
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318415"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..1360)
                     /gene="ltrA"
                     /locus_tag="VF516_43810"
     CDS             complement(44..1360)
                     /gene="ltrA"
                     /locus_tag="VF516_43810"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012826318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEX8114758.1"
                     /translation="MTETQSSLDVSTKLERIAKLAKDKPGVSLLTLAHHIDVDWLREA
                     YRRTRKDGAPGVDGQGATQYAEQLEANLRSLLDRAKSGSYRAPPVRRVHIPKGKGTET
                     RPIGVPTFEDKVLQRAVAMVIGEVYEQEFYDFSYGFRPGRSAHQALQAFQTAAWRMRG
                     GWLVEVDIKKFFDTVDHAYLRDILRQRIGDGVLLRLIGKWLNAGVVEGLMLSYPEEGT
                     PQGGVISPLLANIYLHTVLDEWFVRDVQPVLARKAEMVRFADDLVVLFESKQDAERFL
                     AVLPKRFGRYGLTLHPDKTRMVAFQRPARVDDDENGPGTFDFLGFTHHWAVSRKGNWI
                     VKQRTARDRFSRALRRLREWCRWHRHDPLKEQHRMLTKKLKGHYAYYGITSNFPRISS
                     FYHRARRTWYAALARRSQRRLSWATMMKLLERFPLPAPRIVHRYGT"
     gene            complement(2798..>3470)
                     /locus_tag="VF516_43815"
     CDS             complement(2798..>3470)
                     /locus_tag="VF516_43815"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8114759.1"
                     /translation="ASLVELQAACSMLRSNFGRSDNADEIIKAAGALNRLEATIRLAT
                     QQLHSVRSVQPASSKRRRGLDLAAELDRFASLSGPLLEQHDTTFVVDAPRGSVLRTDM
                     RPELFSAVISSLTLNSLEWRQKGKPMTITAAVRIDGEFVEVTVQDSGKGVTLGLENTV
                     FEPMVSGRDGTGMGLTIARSILETHGGSIELLVDRRRKGATFKIRLPRKKARSTVPTS
                     ARNPS"
BASE COUNT          603 a         1168 c         1079 g          620 t
ORIGIN      
        1 catgcggaat tccacgcatc cggctcttcg acgaacaggt ttgctaggtg ccgtaccgat
       61 ggacgatgcg cggtgcaggg agcgggaacc gctccagcag cttcatcatc gtcgcccagg
      121 acagtcgtcg ttgcgaacgg cgcgcgagcg ccgcgtacca cgtgcgacgt gcccgatggt
      181 agaagctcga gatccgagga aagttcgacg tgatcccgta ataggcatag tgccccttca
      241 gcttcttcgt cagcatgcgg tgctgctcct tcagcgggtc gtgccggtgc cagcgacacc
      301 actcgcgaag tcggcgcagc gcgcggctga agcggtccct ggccgttcgc tgcttcacga
      361 tccagttgcc cttgcgggag actgcccagt ggtgggtgaa cccgaggaag tcgaatgtcc
      421 caggtccgtt ctcgtcgtcg tcgacgcgag cgggccgctg gaacgcgacc atccgggtct
      481 tgtcgggatg cagcgtcagg ccatacctgc cgaatcgctt cggcagcacc gcgaggaacc
      541 gctcggcatc ttgcttgctc tcgaacaaca cgacgaggtc gtcggcgaaa cggaccatct
      601 cggccttgcg cgcgagcact ggttggacgt cccgcacgaa ccattcgtcg agcaccgtgt
      661 gcaagtagat gttggcgagc aggggcgaga tcaccccgcc ttgcggcgta ccctcctcgg
      721 ggtatgagag catgagcccc tcaacgaccc cggcgttcaa ccactttccg atcaggcgca
      781 gcaacacgcc gtcacctatc cgttggcgaa ggatgtcccg caggtaagca tggtcgacgg
      841 tgtcgaagaa cttcttgata tcgacctcga cgagccatcc acctcgcatc cgccacgccg
      901 ccgtctggaa cgcttgcagc gcttggtgtg cggagcgacc gggacgaaag ccgtacgaga
      961 agtcgtagaa ctcctgctcg tagacctccc cgatcaccat cgcgaccgcg cgctggagga
     1021 ccttgtcctc gaaggttgga accccgatcg gccgggtttc cgttcccttc cccttcggga
     1081 tatgcacccg ccgcaccggc ggcgcgcgat agctgccgga cttggcacgg tccaggagcg
     1141 accggaggtt ggcctccagc tgctccgcgt actgcgtcgc accttgtccg tcaacgcccg
     1201 gcgctccgtc cttccgcgtg cggcggtagg cctcgcgtaa ccagtccaca tcgatgtggt
     1261 gggccagcgt caacaggctg accccgggct tgtccttcgc cagtttcgct attcgttcga
     1321 gcttggttga gacgtccagc gagctctgag tctcggtcat cttttccctc caacgagtct
     1381 gttcatcggc gcgctccttt gctcggcagg gtcccctgga gcgggttccc ccacgtcatc
     1441 gctcgtactg cgcgctccga cctctcggcg cccgaccggc tcagtttccc ttgccgtatc
     1501 gttccgcgtt gccgcggagc gccgagactc catcccaagg ttcctgggca acccttgcgt
     1561 acatgccccg ctctcagatc ccggtgagtg gtgcgagtaa gaccgcggcg ctcggccgtg
     1621 ccggtcgcgc atgcagcgca tcctgttgcc ttcgaccttc gaggagatct cggcactcac
     1681 cacggtaacc attttcggga ccccatcacg cggcctgcac gctcgctgtc tacgtgtcac
     1741 caccggggtc gatgggatcc acttccaccg ggatcaccgg ggaccagggg accacccctc
     1801 aaagaagacg gcacagggcg atccgcgggg ataggccggg gtccgaggtc acgaagatcg
     1861 tccgttcggg cgctccggag agagccatcg cctaccgacg tccaggcgcc ggagccctcg
     1921 caactgagat gaccgccgcg ttcgagcgcg cagagcctcg aaggcaagcg cgccggctgc
     1981 acgtgccgcg ttgctcacca agctccgcat caacagcgag gacaccgcgc gcgacacgaa
     2041 gcgcagtggc gagctccacg acgcgaacac gggttcccag acacaagggg cggtcacctc
     2101 cgaagctgcg ctcagacgtt ccgcgaacag agcttcgtct tgctcgcatc gctgccaggc
     2161 cgcttcctcg tcgaggaata tgaattgtcc gaattacgga cagtatgtcc ggagagcgga
     2221 catctgattc agcatgtcat gacgtcgcgg cttcgacgct ggttcgatgt ggcccaaggc
     2281 ggaccctgcc gcgcggccga cgcgatgccg cactggacag catcgaggag ctgtgagatc
     2341 actacgacga agcgcagtcc caagagggac cacccgccgt tcacgagaag cggcgggcga
     2401 acacgacgcc caccccctac cgccggtcga ccttcggtgg aggcgaagaa ccgtctgcgc
     2461 gccaccgggt gggtccctgg atcccggtga cgccggtgga tctggtaccc tcgctcgccg
     2521 gtggcgacag ctactcgcaa gaagctggga agcaaactcc acaacccgac ggcgaagggc
     2581 cacctcgagt cgagctgacg gacacgcttg cggaccgcgt cggtctagag cagcgcggcg
     2641 gcggtccagc tactggccgc cgaggtcgac gaacgctcgg gacgcttgag aacctcttgg
     2701 gcgcgatcac gagccgctgt gcccgctcgc tagcatcgtg cgcatctcag aacgtctgag
     2761 ttctaagtcg ccagtacccc ttgcgccgcc gacgcgttca gctcggattt cgcgccgacg
     2821 tcggtaccgt ggaacgagcc ttttttcgcg gcagacggat cttgaaggtc gctcccttcc
     2881 gccgccggtc gacaagcaac tcgatgctgc cgccgtgcgt ttctaggatg ctccgcgcga
     2941 tggtgagccc catgcctgtc ccatcacgac cggagaccat cggctcgaag acggtgtttt
     3001 ccagaccaag ggtgaccccc tttccggaat cctgcacggt cacctcgaca aattcaccat
     3061 cgatacgcac cgccgcagtg atcgtcatcg gcttcccctt ctgccgccac tcgagcgaat
     3121 tgagcgtgag gctgctgatc acggcgctga agagctccgg cctcatgtcg gtgcgcagta
     3181 cagagccgcg aggcgcgtcg accacgaacg tggtgtcgtg ttgctccagc agggggccgc
     3241 tcagggacgc gaagcgatcg agctcggcgg ccaggtcaag cccgcgtcgc cgcttcgacg
     3301 atgctggctg cacggaccgc acggagtgga gctgctgggt tgcaagacgg attgttgcct
     3361 cgagccggtt cagcgctcca gccgccttga tgatctcgtc ggcgttgtcg ctgcgcccga
     3421 agttggagcg caacatcgag catgctgcct gcaattccac aagagaggct
//
LOCUS       DASVOR010002378         4183 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_552739,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002378 DASVOR010000000
VERSION     DASVOR010002378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552739"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..894)
                     /locus_tag="VF516_43820"
     CDS             complement(<1..894)
                     /locus_tag="VF516_43820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114760.1"
                     /translation="MANRFGAYEILYELKTGGMGAVLLGRRRGPGAFEQLVAIKTIRP
                     EFAQTAAVRAMFLDEAAILARLNHPGVATVHDFGEQGGTLYMVMEYVAGISFRDAMQH
                     GMPPVVAARAIAEACRGVHAAHELRDLAGNLLGVVHRDISPDNLMIGFDGHVKVIDFG
                     IALIKNRQAPVTELGTVKGKPPYMSPEQVKNEAMDRRSDVFSLAVVLWEMLTGRGLFE
                     GDSIYAIALAVEHQRLVPPSQVLGAPLPRGLDAVVMNALDRDLGRRTRTAAQLAEQLE
                     QVIQTAGDPTLEAWAERALDEP"
     gene            complement(931..2298)
                     /locus_tag="VF516_43825"
     CDS             complement(931..2298)
                     /locus_tag="VF516_43825"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8114761.1"
                     /translation="MTLDRRTVIQTGLATSTTRGRTAPRSYRVQVLGHGGATIALGAR
                     PVVVGAHASCDLVLSDPQVSRRHAELAAVPEGIRIKDLGSTNGTWWQGTKVGEVVVPS
                     GATVQFGVTSVRIAAAEAIALPPSEHDHFGAMAGTSVAMRELFAVLEMASPTDATVLI
                     EGESGTGKELAARAIHEASGRAGGPFVVVDCSAITENLIDSHLFGHVRGAFTGADRDR
                     RGAFVEASGGTLFLDELGELPLAAQAKLLRVLEAQTVQPVGADRPVSVDTRVVAATHR
                     DLTRMVAAKEFRFDLFYRLAVVHVALPPLRERFEDLPHLIATFYRARGLEPGPIDGDN
                     LERMRRHAWPGNVRELRNALERAWALSGAGARFSGLRLWLDPNAAPQLAEVIDTALPF
                     KEAKERWNDHFERRYVAMVFARSASNVTRAAEHAGLSRRHFRELLYKHGIVERPTDDS
                     EDGPA"
     gene            2365..4071
                     /locus_tag="VF516_43830"
     CDS             2365..4071
                     /locus_tag="VF516_43830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114762.1"
                     /translation="MRWCGWLLVAACSRDPSLAVTVHHPAGYAVTQTRVTAYVGDGVQ
                     CSEIEFGDRTEAELEALAVSDAIDGGEIEVSRLGEKAIVARGYDAQGRFVTAGCKDVG
                     EIAGATTVAITTQPTASVAIDPGQPDRPFGEREILVNMTDPKGKPLDGTVSWQVTGPG
                     GTVPQMASAGVPTKNGNLRFQVTDIGQPGPEGLRIRAPWATAPLPLVTAFDLSHATTV
                     QLGGGAAASHPSCDVRGHAGKPPTLVCLTQADVVNGHRDLVEIGSQGGRFAAMPAITL
                     PGNNQFALFVDHDGSADEPVYVLSANAAGAGSWYKLGASSTAETFASALQNVVYIPKC
                     ADNSNQALVAVQTGTGLGVANQIRIFTVAGTAIGSAPSDGEVFSGGCIHDVDKKEHQG
                     VVVASASGDAGLELITPGSPTFMQIMSAKFTGSGFVTIESQGVIEKRFAGTRLQATGT
                     VVFEAVLAVQGSSYMLVERTEVEAAAPPEKIIAGKLDLDSDTDLMWDMGIGSRRRVFQ
                     VSLAEQVGGAPLTAMTSGPGGLSTATASPTDFVVGDLDGKGADEMILFTQSAVTIYSP
                     DE"
BASE COUNT          597 a         1506 c         1518 g          562 t
ORIGIN      
        1 cggctcgtcc agcgcgcgct cggcccacgc ttcgagcgtc gggtcgcccg cggtctggat
       61 cacctgctcg agctgctcgg cgagctgggc ggcggtccgg gtccggcgcc cgaggtcgcg
      121 atcgagcgcg ttcatcacca ccgcgtcgag cccgcgcggc agcggcgcgc cgagcacctg
      181 cgacggcggc acgagccgct ggtgctcgac cgcgagcgcg atcgcgtaga tcgagtcgcc
      241 ctcgaacagc ccgcggccgg tcagcatctc ccacagcacg acggcgagcg agaacacgtc
      301 gctgcgccgg tccatcgcct cgttcttgac ctgctcgggc gacatgtacg gcggcttgcc
      361 cttgaccgtg ccgagctcgg tgaccggcgc ctggcggttc ttgatcagcg cgatgccgaa
      421 gtcgatcacc ttgacgtggc cgtcgaaccc gatcatcagg ttgtcggggc tgatgtcgcg
      481 gtggaccacg ccgagcaggt tgccggcgag gtcgcgcagc tcgtgggcgg cgtggacgcc
      541 gcggcacgcc tcggcgatcg cgcgcgcggc gaccaccggc ggcatgccgt gctgcatcgc
      601 atcgcggaac gagatcccgg cgacgtactc catcaccatg tacagcgtgc cgccctgctc
      661 gccgaagtcg tgcacggtcg cgaccccggg gtggttgagc cgggcgagga tcgcggcctc
      721 gtcgaggaac atcgcgcgca ccgccgcggt ctgtgcgaac tcggggcgga tggtcttgat
      781 cgcgaccagc tgctcgaacg cgcccggccc gcgccggcgg ccgagcagca ccgcgcccat
      841 gccgccggtc ttgagctcgt agaggatctc gtaggcgccg aatcggttgg ccacgtgccg
      901 ccacacgata ccgcggcggc cggcccggcg tcaggccggt ccatcctcgc tgtcgtcggt
      961 ggggcgctcg acgatgccgt gcttgtacag cagctcgcgg aagtgccggc gcgacagccc
     1021 ggcgtgctcg gcggcgcgcg tgacgttgct cgcgctgcgg gcgaacacca tcgcgacgta
     1081 gcggcgctcg aagtgatcgt tccagcgctc cttggcctcc ttgaacggca gcgcggtgtc
     1141 gatcacctcg gcgagctggg gcgccgcgtt gggatcgagc cacagccgga ggcccgagaa
     1201 ccgcgcgcct gcccccgaca gcgcccaggc gcgctccagg gcgttgcgca gctcgcggac
     1261 gttgccgggc caggcgtggc ggcgcatccg ctcgaggttg tcgccgtcga tcggcccggg
     1321 ctcgaggccg cgcgcccggt agaacgtcgc gatcaggtgc ggcaggtcct cgaaccgctc
     1381 gcgcagcggc ggcagcgcga cgtggaccac cgcgagccgg tagaacaggt cgaaccggaa
     1441 ctccttggcc gcgaccatgc gcgtgaggtc gcggtgggtg gcggcgacca cgcgcgtgtc
     1501 gacggagacc ggccgatcgg cgccgaccgg ctgcacggtc tgggcctcga gcacgcgcaa
     1561 cagcttggcc tgcgccgcga gcggcagctc gccgagctcg tcgaggaaca gcgtgccgcc
     1621 gctggcctcg acgaacgcgc cgcggcgatc gcggtcggcg ccggtgaacg cgccgcggac
     1681 gtggccgaac aggtgcgagt cgatcaggtt ctcggtgatc gcgctgcagt cgaccacgac
     1741 gaacggcccg ccggcgcggc cgctggcctc gtggatcgcg cgcgcggcga gctccttgcc
     1801 ggtgccggac tcgccctcga tcagcaccgt cgcgtcggtc ggcgacgcca tctcgagcac
     1861 cgcgaacagc tcgcgcatcg ccacgctggt ccccgccatc gcgccgaagt gatcgtgctc
     1921 gctcggcggc agcgcgatcg cctcggcggc tgcgatccgc accgaggtga ccccgaattg
     1981 cacggtggca ccggaaggga ccaccacctc tcctaccttg gtgccctgcc accacgtgcc
     2041 gttggtcgag ccgaggtcct tgatccggat cccctccgga accgccgcga gctcggcatg
     2101 tcggcgagac acctgcggat ccgacagcac caggtcgcac gaggcgtgcg cgccgaccac
     2161 caccggccgg gcgccgagcg cgatcgtcgc cccgccgtgg cccaggactt gaacccgata
     2221 cgatcgcggg gcggtccggc cccgggtcgt gctcgtcgcg agcccggtct ggatcaccgt
     2281 ccgccgatcg agggtcacat gcacagctta acccgtgccg cccggaccag cgtcctaggc
     2341 gacatgaatg atacggtccg cgcgatgagg tggtgcggat ggctcctggt ggcggcctgc
     2401 agccgtgatc cgagcctcgc ggtcacggtc caccaccccg cggggtacgc ggtcacccag
     2461 acccgcgtca cggcgtacgt cggcgacggc gtgcagtgca gcgagatcga gttcggcgat
     2521 cgcaccgagg ccgagctcga ggcgctcgcg gtcagcgacg cgatcgacgg cggcgagatc
     2581 gaggtcagcc ggctcggcga aaaggcgatc gtcgcgcgcg gctacgacgc gcagggccgg
     2641 ttcgtgaccg ccggctgcaa ggacgtcggc gagatcgccg gcgcgaccac cgtggcgatc
     2701 accacccagc cgaccgcgag cgtcgcgatc gatccgggtc agcccgaccg gccgttcggc
     2761 gagcgcgaga tcctggtcaa catgaccgat ccgaagggca agccgctcga cggcacggtg
     2821 tcgtggcagg tgaccggccc gggtggcacg gtgccgcaga tggcgtccgc cggggtgccg
     2881 accaagaacg gcaacctccg gttccaggtc accgacatcg gtcagcccgg ccccgagggc
     2941 ctgcggatcc gcgcgccgtg ggcgaccgcg ccgctgccgc tggtcaccgc gttcgacctg
     3001 tcgcacgcga cgacggtgca gctcggcggc ggcgccgcgg cgagccatcc atcgtgcgac
     3061 gtgcgcggcc acgccggcaa gccgccgacg ctggtctgcc tgacccaggc cgacgtcgtc
     3121 aatggccacc gcgacctggt cgagatcggc tcgcagggcg ggcggttcgc ggcgatgccg
     3181 gcgatcacgc tcccggggaa caaccagttc gcgctgttcg tcgatcacga cggcagcgcc
     3241 gacgagccgg tctacgtgct gagcgcgaac gccgccggcg ccggcagctg gtacaagctc
     3301 ggcgcgtcga gcaccgccga gacgttcgca agcgcgctgc agaacgtcgt ctacatcccg
     3361 aagtgcgccg acaactcgaa ccaggcgctg gtcgcggtgc agaccggcac cgggctcggc
     3421 gtcgccaacc agatccggat cttcaccgtg gccggcacgg cgatcggctc cgcgcccagc
     3481 gacggcgagg tgttctccgg cggctgcatc cacgacgtcg acaagaagga gcaccagggc
     3541 gtggtcgtgg cgagcgcgag cggcgacgcc ggcctggagc tgatcacccc ggggtcaccg
     3601 accttcatgc agatcatgag cgcgaagttc accggctcgg ggttcgtcac gatcgagagc
     3661 cagggcgtga tcgagaagcg gttcgccggc acccggctgc aggcgaccgg caccgtggtg
     3721 ttcgaggccg tgctcgcggt gcagggcagc tcgtacatgc tggtcgagcg caccgaggtc
     3781 gaggccgcgg cgccgcccga gaagatcatc gccggcaagc tcgatctcga cagcgacacc
     3841 gatctgatgt gggacatggg gatcggctcg cgccggcggg tgttccaggt ctcgctcgcc
     3901 gagcaggtcg gcggcgctcc gctcaccgcg atgaccagcg ggccgggcgg gctgtccacg
     3961 gccaccgcga gcccgaccga cttcgtcgtc ggcgacctcg acggcaaggg cgccgacgag
     4021 atgatcctgt tcacccagag cgccgtgacg atctactcgc ccgacgagta gctcgcgccg
     4081 gggcggcgtg ccaggatgcc gcgatgctgc gcgtgatgtc gatcgccgcg gtgctcgcgg
     4141 cgtgcagcgg ccgagcgccc ggcccgcccg cgtcgtcgcg gcc
//
LOCUS       DASVOR010002379         2359 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_287889,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002379 DASVOR010000000
VERSION     DASVOR010002379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287889"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..826
                     /locus_tag="VF516_43835"
     CDS             <1..826
                     /locus_tag="VF516_43835"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HEX8114763.1"
                     /translation="TSVVTVRDAGGTSQGAFDASHVGPAIDAARLVPPSLRAALRGCS
                     EVEVLARPPVQGLPQLLADDVAWSYRVDRDRRPTAPGSAPARATRLVIANTEPPASLG
                     VPRLLPWQSSEAPDVRLEGAAATPSRALAEFTDATFIEVHSHGVADTTGADAAFLMLS
                     PEPDGRYALTAAEIRQHPLRGRPIVILAACHAATTATYRHEAWSLPAAFVAAGARAVI
                     ASTDVIDDAAAGAFFDDMRARIEHGASPAVALRDVRVTWLASHPGATWVHSLMVFE"
     gene            894..1445
                     /locus_tag="VF516_43840"
     CDS             894..1445
                     /locus_tag="VF516_43840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114764.1"
                     /translation="MLSLATVVGCSRAAQPQQPTSPPLQQLTGPPLATPWTMPKPGER
                     VFSESAQRVQVIVTPGRRKGEQYAWVFSNGRKLEHFYRFTNNEANQLVAHLNDLQRTL
                     GFSFSVVYHSCPAKPKTVGERHRSSPRPPSSEFAQTLPADGDPDTCGGSTTGGTTGYG
                     GQPGEPPPPLTQAIVTDALDSSL"
     gene            complement(1501..2151)
                     /locus_tag="VF516_43845"
     CDS             complement(1501..2151)
                     /locus_tag="VF516_43845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEX8114765.1"
                     /translation="MRTLALWFLISSTAAAAVACHGDDDAQEDAPAAVKLPRRAVPQI
                     PPPLDLRTPPADATRTASGLIYKPLGGGTTGAQPRSDDTVLVRYTGWRQHTGETFFTT
                     TGRAQPIAIDLAHAAPGFREALPLLHAGEKAVLWMPPRPGTLEPVAYEVELVEVVASR
                     PAEGASSAHPPASESRATSPGDIDGDGGSSGTGWPGDGNPPGTPPPPETSSSNAPS"
BASE COUNT          351 a          840 c          812 g          356 t
ORIGIN      
        1 caccagcgtc gtgacggtgc gcgacgcggg cggcacgagc cagggcgcgt tcgacgccag
       61 ccacgtgggg cctgcgatcg acgccgcgcg gctcgtcccg ccgtccctcc gggcggcgct
      121 ccgcggctgt tccgaggtcg aggttctcgc gcgcccgccg gtgcagggtc tgcctcagct
      181 gctggccgac gacgtcgcgt ggagctatcg cgtcgatcgc gatcgccggc ccactgcccc
      241 gggctcggcc cccgcgcgtg cgacccgcct cgtgatcgcc aacaccgagc caccggcatc
      301 gctcggcgtt cctcggctcc tgccgtggca gtcgtccgag gctcccgatg tccggctcga
      361 gggggcggcg gcgacgccgt cgcgggcgct cgccgagttc accgacgcga ccttcatcga
      421 ggttcactcg cacggtgtgg cggacaccac cggcgccgac gccgcgttcc tgatgctgtc
      481 ccccgagccc gacggtcgct atgcgctgac cgccgccgag atccggcagc atccgctgcg
      541 cgggcggccg atcgtgatcc tcgcggcctg ccacgctgcg acgaccgcga cctatcgcca
      601 cgaggcctgg agcctgcccg ctgccttcgt cgccgccggc gcgcgcgccg tgatcgcctc
      661 gaccgacgtg atcgacgatg ccgccgccgg cgcgttcttc gatgacatgc gcgcgcgcat
      721 cgagcacggt gcgtcgcccg cggtcgcgct acgtgatgtc cgcgtcacct ggctggcctc
      781 gcacccgggc gcgacctggg tgcactcatt gatggtattc gagtaggacg ggatgatcag
      841 gaagggatga ccaggaaggg atgatcagaa tgagacatcg aatgatgata tgtatgctga
      901 gcctggcgac cgtggtcggc tgcagtcgtg cggcccaacc acaacagccg acgagtccac
      961 cgctgcagca gttgaccgga cccccgctgg cgacgccgtg gacgatgccc aagcccggcg
     1021 agcgggtgtt ctcggaatct gcccagcgcg tgcaggtcat cgtcacgccc ggccggagga
     1081 agggcgagca gtatgcatgg gtgttctcga acggtaggaa gctcgagcac ttctatcgct
     1141 tcaccaacaa tgaagcgaac cagctcgtcg ctcacctcaa tgatctgcaa cggacgctgg
     1201 ggttctcgtt cagcgtcgtc tatcacagct gcccggcgaa gccgaagacg gtcggggagc
     1261 gccaccggag ttcgccacgt cccccgtcca gcgagttcgc acagaccctt ccggcagatg
     1321 gcgatcccga tacctgcggt ggctcgacca cgggaggaac cacgggttac ggaggacaac
     1381 ccggcgagcc cccgcccccg ctgacccagg ccatcgtgac ggatgcgctg gatagcagcc
     1441 tgtagccgtg accgatccgc gcgaacgccg cgccggcagc cacggcgggc cgactcgcga
     1501 tcagctcggt gcgttcgagc tcgaggtctc cggtgggggc ggtgtgccag gagggttacc
     1561 gtccccgggc cagcccgtgc ccgacgagcc gccgtcgcca tcgatgtcgc ccggtgatgt
     1621 ggcccgggat tcgctggccg gcggatgcgc cgagctcgcg ccctcggcgg ggcggctcgc
     1681 gaccacctcg acgagctcga cctcgtaggc caccggctcc agggttccgg gccgcggcgg
     1741 catccacagc accgccttct cgcccgcatg cagcagcggc agcgcctcgc gaaaccccgg
     1801 ggctgcgtgc gccaggtcga tcgcgatcgg ctgtgcgcga ccggtcgtcg tgaagaacgt
     1861 ctcgccggtg tgctgccgcc agccggtgta ccggaccagc accgtgtcat cgcttcgcgg
     1921 ctgggcgccc gtggtaccgc cgcccagcgg cttgtagatc agccccgacg ccgtcctcgt
     1981 cgcatcggcc ggcggcgtgc gaaggtcgag cggcggcggg atttgcggga ccgcgcgccg
     2041 cggaagcttc accgcggcgg gagcgtcctc ctgggcgtca tcatcgccgt ggcacgcgac
     2101 agccgccgcg gccgtcgacg agatcaaaaa ccagagagca agggtgcgca tggtcccgac
     2161 cctacggtgc gcatcgcgag atcgccagcg ctggaccgtg gtgcgcggtc gaattgacgg
     2221 gttgatccga aaatcgcaac gacccgtcat gacgggttga tccgaaaatc gcgacgaccc
     2281 gtcaattcga ccgccgcgtc gcgcaggcct gatgcattgt cgcggcggct catttaatgc
     2341 tgttcccgaa agggacagg
//
LOCUS       DASVOR010002380         4089 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_201525,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002380 DASVOR010000000
VERSION     DASVOR010002380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201525"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..417)
                     /locus_tag="VF516_43850"
     CDS             complement(109..417)
                     /locus_tag="VF516_43850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114766.1"
                     /translation="MSVLAPTPPDRMTDASGRPYFLWDDDMSLDSFRANLRHPDPEVR
                     AYFIGKLMRQAKPDDVFSFVALREIEDQWDSIERYLGHSREFWRWLLERWATYPHADR
                     "
     gene            complement(792..2036)
                     /locus_tag="VF516_43855"
     CDS             complement(792..2036)
                     /locus_tag="VF516_43855"
                     /inference="COORDINATES: protein motif:HMM:NF017442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF790 family protein"
                     /protein_id="HEX8114767.1"
                     /translation="MLTADLVRARRRGDELTLTSLDAGGRATALELAATLLGLAEAHV
                     GKTRGELADAMDAVAGGAKDRKLAAGLAKLVWDRVELVEAVPIDPEALRHDVFARAQA
                     AWAQIGEGDRFDRCAILAAVAADRGLTVDEIERGLYADLRDAHVITRLAPITAPQLVA
                     AYDLAQPQAVLLRAVRVIVEVESAIPGAYRTLFRKLKFLRLLCAIAPRAKGGYRIEID
                     GPFSLFESSTRYGLALALALPAIRELDRWSLVADLRWGTARTPLRCRLDGRGEPDPEA
                     APHLADDVRALLDALRALDTPWQVTPSVEILDLPGHGVCIPDLTFEHAQTGERVHLEV
                     LGYWSRDAVWKRVELVEAGLAHRIVFAVGQHLRVSEAALDAELPAALYVYKRTMSAKA
                     IVERVDALATVRVLRKRGAARP"
     gene            complement(2023..3375)
                     /locus_tag="VF516_43860"
     CDS             complement(2023..3375)
                     /locus_tag="VF516_43860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEX8114768.1"
                     /translation="MPLSLTFVAGTLELRGLAADAPAPAGCLWDPRAACHRAPAIAYA
                     ELVRALTRDKVAHDDRARAYPTLEHGMRVRRAPRPYQIEALAAWQGQRSRGVVVLPTG
                     AGKSQLACMAIDAKRRSTLVVAPTLDLVRQWYDLLRTTFGVVIGVVGGGDHDVQPLTV
                     TTYDSAYLHMEHLGARFGLLVFDECHHLPGTSYALAARSALAPFRLGLTATPDREAAL
                     DELIGPVAYRKEITDLAGDFLAEYDIERVEVELSPEERAEHDTERAIYRAFVERHGIR
                     MSSAHGWSEFIMKSAGSLEGRRAMAAYRRQRQLAFCAPAKLDFVESLLARHHAERTLL
                     FTSDNATCYEVSRRFLIPAITHQTKVKERSALLAHLADGTYRALVTSRVLNEGVDIPA
                     ASVAIVVSGSGAVREHVQRLGRILRPLAGKRATLYELITANTAEQYTSERRRDHVAYR
                     "
     gene            3526..3957
                     /locus_tag="VF516_43865"
     CDS             3526..3957
                     /locus_tag="VF516_43865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114769.1"
                     /translation="MLDHRENGFHVDPLDLRHLAENAAALVVAGATLPSADRDSLRLA
                     IGRDTNLLFHHGRYSGWILGSPIAHLVSEPGEPAPGTDDPRLHEPLREYLALVVEPNI
                     TRMCDEDPRPAGGAVRATSPRPKRRRRAGLCSRERHRARHW"
BASE COUNT          674 a         1346 c         1487 g          582 t
ORIGIN      
        1 gctcggtggt gcgtatgaaa gccggcgagc gcggcaccgc cggtcagcgt ccatggcggc
       61 tcgatgtcgg cgagcagccg cagaacacgc tcctgcagcg acgagagctt accgatcggc
      121 atgaggatag gtggcccatc gttcgaggag ccatcgccag aactcgcgcg agtggcccag
      181 gtagcgctcg atcgaatccc actggtcctc gatctcccgc agcgccacga acgaaaacac
      241 gtcgtcgggc ttggcctgac gcatcagctt gccgatgaag tacgcgcgaa cctccggatc
      301 gggatggcgc aggttcgcgc ggaacgagtc cagcgacatg tcgtcgtccc acaggaagta
      361 aggccgcccc gacgcgtcgg tcatccgatc gggcggcgtg ggagcgagga cggacatggc
      421 gcctccagca tagacgacag cgcgttctac cccaagtcga gagcagtcca ggccaccgga
      481 tgggtcgcgc tcgccgcgga cgatgccgtc aggaaccgct ggcgcccggc gagaaaccgt
      541 tcgcgaagcg cgtcctccgc ccagaccacc ttcctgatct gattcgcgag agaggcatca
      601 atcgggtgat ctggcatcgg acgtggaaag cgagggacga tgctcctctg atcgttcacc
      661 catgtgagca acgcccccca cgctcggtag aacaacgcgg cgtcttccgg taggagaagc
      721 atgcgtttcc atcgcaagct tttgcccggc gggcaaggcc gagagacgag tgattcggac
      781 ggccaggatg gtcagggcct ggcggccccg cgcttgcgga gaacacgaac cgtggcgagc
      841 gcatcgacgc gctcgacgat cgccttcgcg gacatcgtgc gcttgtagac gtacagcgcg
      901 gcggggagct cggcgtcgag tgcagcttcg ctgacgcgga ggtgctggcc gacggcgaac
      961 acgatgcggt gggcgagacc ggcctcgacc agctcgacgc gcttccacac ggcgtcgcgg
     1021 ctccagtagc cgaggacctc gagatggacg cgctcgccgg tttgcgcatg ctcgaaggtg
     1081 aggtcgggaa tgcacacgcc atggcccggc agatcgagga tctccaccga gggtgtcacc
     1141 tgccatgggg tgtcgagcgc acgcagcgca tcgagcagcg cgcgcacgtc gtcggcgagg
     1201 tgcggcgcgg cctcgggatc gggctcgccg cggccgtcga ggcggcagcg cagcggcgtg
     1261 cgggcggtgc cccagcggag gtcggccacg agcgaccacc ggtcgagttc gcggatggcg
     1321 gggagtgcaa gggcaagcgc gaggccgtag cgggtcgacg actcgaacag cgagaacgga
     1381 ccgtcgatct cgatccggta tccgcccttg gcacgcggcg cgatcgcgca cagcaggcgc
     1441 agaaacttga gcttgcgaaa cagcgtgcgg taggcaccgg ggatcgcgct ctcgacctcg
     1501 acgatgacgc ggacagcgcg gagcaggaca gcctggggct gggcgaggtc gtaggcggcg
     1561 acgagctgcg gagcggtgat cggtgccagc cgggtgatca cgtgagcatc gcggaggtcg
     1621 gcgtagagac cgcgctcgat ttcgtcgacg gtgagcccgc gatcggcggc gacggcagcg
     1681 aggatggcgc accgatcgaa gcgatcgcct tcaccgatct gtgcccacgc ggcctgcgcg
     1741 cgcgcgaaca cgtcatggcg gagcgcctcg ggatcgatcg gtacggcctc gacgagctcg
     1801 acgcgatccc agaccagctt ggcgaggccg gcggcgagct tgcgatcctt ggcgccgccg
     1861 gcgaccgcgt ccatggcatc ggcgagctcg ccgcgggtct tgccgacgtg cgcctcggcg
     1921 aggccgagca aggtcgccgc cagctcgagc gcggttgcgc ggccgccagc gtcgagcgac
     1981 gtcagcgtga gctcatcgcc ccgccggcgg gcgcggacca gatcagcggt aagcaacgtg
     2041 atccctccgc cgctcgctgg tgtactgctc ggcggtgttg gcggtgatca gctcgtagag
     2101 cgtcgcccgc ttgccggcga gcgggcgaag gatgcggccg aggcgctgga catgctcgcg
     2161 cacggctccg gatccggaga ccacgatcgc cacgctcgcc gccggaatat cgacgccctc
     2221 gttgaggacc cgggacgtca ccagcgcgcg ataggtgccg tcggccaggt gggcgagcaa
     2281 cgcgctgcgc tccttcacct tggtctggtg cgtgatcgcc gggatgagga agcggcgcga
     2341 gacctcgtag caggtcgcgt tgtcggaggt gaacagcagc gtacgctcgg cgtggtggcg
     2401 cgcgagcagg ctctcgacga agtcgagctt ggcgggggcg cagaacgcga gctggcgctg
     2461 gcggcggtag gcggccatcg cgcggcggcc ctcgagggag cccgccgact tcatgatgaa
     2521 ctcggaccag ccgtgcgcgc tcgacatccg gatgccgtgc cgctcgacga acgcgcggta
     2581 gatcgcgcgc tcggtgtcgt gctcggcgcg ctcttccggc gacagctcga cctcgacccg
     2641 ctcgatgtcg tactcggcga ggaagtcgcc ggcgaggtcg gtgatctcct tgcgataggc
     2701 caccggcccg atcagctcgt caagcgcggc ctcgcggtcg ggcgtcgccg tcaggcccag
     2761 gcgaaacggc gcaagcgccg agcgcgcggc gagtgcatac gacgtgcccg ggaggtggtg
     2821 acactcgtcg aagacgagta acccgaaccg cgcgccgaga tgctccatgt ggaggtaggc
     2881 cgagtcgtag gtcgtgacgg tgagcggctg gacgtcgtgg tcgccgccgc cgaccacgcc
     2941 gatgacgacg ccgaaggtcg tgcgcaagag gtcgtaccac tggcggacca gatcgagcgt
     3001 cggtgcgacc accagcgtcg atcggcgctt ggcgtcgatc gccatgcagg cgagctgcga
     3061 cttgcccgcc ccggtgggaa ggacgacgac accgcggctc cgctggccct gccacgcggc
     3121 gagcgcctcg atctggtaag gccgcggcgc ccggcgaacg cgcatgccgt gctcgagcgt
     3181 cgggtacgcg cgagcccggt catcgtgcgc gaccttgtcg cgggtgaggg cgcggaccag
     3241 ctcggcatag gcgatcgccg gtgcgcggtg gcacgccgcg cgcggatccc agaggcatcc
     3301 agccggcgcc ggcgcgtccg cggcaaggcc acgcagctcc agcgtcccgg cgacgaaggt
     3361 caacgacagc ggcatcgagg cgctggatag acaatcacat ggcgctggcg gcggcaacgc
     3421 tgcgtcgaac tcggcgcgag cctcgacgat gctccgacgt ggtcggcact cgagccggcg
     3481 atcgcacgat catggctcgc cctaccccga cggaccggga tcccggtgct cgaccaccgc
     3541 gagaacggct tccacgtcga tccgctggac ctgcgacatc tcgccgagaa cgcggccgcg
     3601 ctcgtcgtcg cgggcgcgac actgccgagc gccgatcgcg actcgttgcg tctcgcaatt
     3661 ggccgggaca ccaacctgct gtttcatcac gggcgctaca gcggctggat cctcggttcg
     3721 ccgatcgccc acctggtatc ggaaccgggc gagcccgcgc cgggtacaga tgatccgcgc
     3781 cttcacgagc cgctccggga gtacctcgcg cttgtcgtgg agcccaacat cacgcggatg
     3841 tgcgatgaag atcccagacc tgcgggaggc gctgtgcgcg ctacgagccc gcgtccgaag
     3901 cgtcgacgga gggcaggcct gtgctctcga gagcggcatc gagcgcgtca ctggtagaac
     3961 acgctcggct gcaggttctc ctcgttgcac aagtccgaca ccgggacctt gtcgaccatg
     4021 tggcgcttga ggatcgcgac cttctgctcg gtggtgaagt ggcggcggga acgcttggaa
     4081 ctcgacatc
//
LOCUS       DASVOR010002381         2854 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_522379,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002381 DASVOR010000000
VERSION     DASVOR010002381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522379"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(67..1329)
                     /locus_tag="VF516_43870"
     CDS             complement(67..1329)
                     /locus_tag="VF516_43870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8114770.1"
                     /translation="MPSRLHPAWTVAGATFVVLLVGAAIRATPGVLMLPLEHELGASR
                     ATIALAGSINLLLYGLMGPFCAAIAERLGVKRMIALAMAMLAAAILAATQITALWQYI
                     ALWGLVVGVATGMVANVLGAVIATRWFVRHRGLVLGLLTAATATGQLLFLPVIAVLTA
                     GPGWRWALVVVGGAALVMVPLALLFVRDHPADAGLRPYGAGPDDPPPARRAGNPVALA
                     FQVLARASRSRDFWLLAGTFFVCGASTNGLIGQHFIPACMDHGMPEVTAAGMLALMGV
                     FDIFGTTGSGWLSDRVDSRWLLFTYYGLRGLSLIFLPRALLEQQGLSVFTVFYGLDWI
                     ATVPPTVKLSANAFGPRDAPIVFGWVVAAHQVGAAFASEAAGIIRSEAGDYRYAFVSS
                     GLLCLVAAGFALTIARGARSAPAAQPAG"
     gene            complement(1418..>2854)
                     /locus_tag="VF516_43875"
     CDS             complement(1418..>2854)
                     /locus_tag="VF516_43875"
                     /inference="COORDINATES: protein motif:HMM:NF016190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEX8114771.1"
                     /translation="LEAIAAARPGGGESYSGGGGHSGSGGGGGGGGVSFELVYWLFRL
                     IFAYPAIGLPIVGLIVGYVIVSAYRQQQNKDWDSGPPATLHDAIELSDVRRLDPAFSQ
                     VVFEDFAFRLFSTAHRARHTAEALATVAPYVSADARAELAEHGPAGQPLVQVVVGALR
                     VIRVELPDAPTGRDGRPRRVRVQVEYEANLATTDHTSYTVETWVFGRDATVQSKPPGA
                     TRTFPCPHCGAPWQASRTTTQVCASCGQAVDNGRFDWIVEDVALSSSDERPPTLTAEV
                     PERGTDLPTYREPGVDAAWRALTGDDPEVTDAALDARLAMIYRELNAAWSNNDLHPVR
                     GLVSDGLYDYLSYWVDAYREQGLRNVLVDMRITRTALAKLVRDRYYDAITIRLWATGK
                     DHVIRTQTGALVRGSKHRERPYSEYWTLIRSAGHKGAPRTQPACSHCGAPLQINQSGE
                     CEHCGAHVTSGEFDWVVSKIEQDDTYRG"
BASE COUNT          421 a          996 c         1087 g          350 t
ORIGIN      
        1 cttcgcgatt ggactcttga ttgttggtct cgggagcgtc gagtcgatgg cggaatttcg
       61 aaggcgtcag ccggccggct gcgcggcggg tgcggagcgc gcgccgcggg cgatcgtgag
      121 ggcgaacccg gcggcgacca ggcagagcag gcccgacgac acgaacgcgt agcggtagtc
      181 gccggcctcg gagcggatga tcccggcggc ctccgaggcg aacgcggcgc cgacctggtg
      241 cgcggcgacc acccagccga acacgatcgg cgcgtcgcgc gggccgaacg cgttggccga
      301 cagcttgacg gtgggcggca cggtcgcgat ccagtccagg ccgtagaaca cggtgaacac
      361 cgacaggccc tgctgctcga gcagcgcgcg cggcaggaag atcagcgaca ggccgcgcag
      421 gccgtagtac gtgaacaaga gccaccggct gtcgacccgg tcggacagcc agcccgatcc
      481 ggtggtgccg aagatgtcga acacgcccat cagcgccagc atgccggcgg cggtgacctc
      541 gggcatgccg tggtccatgc acgcggggat gaagtgctgg ccgatcagcc cgttggtgct
      601 ggcgccgcac acgaagaacg tgccggcgag cagccagaag tcgcgactgc gcgacgcacg
      661 cgccaggacc tggaacgcga gcgccaccgg gttgccggcg cggcgggccg gcggcggatc
      721 gtcggggccc gcgccatagg gccggagccc ggcatcggcc gggtggtcgc ggacgaacag
      781 cagcgcgagc ggcaccatga ccagcgccgc gccgccgacc acgacgagcg cccagcgcca
      841 gcccggtccg gccgtgagca cggcgatcac cggcaggaac agcagctgcc cggtggcggt
      901 ggcggcggtc aagaggccga gcaccaggcc gcggtggcgg acgaaccagc gggtcgcgat
      961 caccgcgccc aggacgttgg cgaccatccc ggtggcgacg ccgaccacca ggccccacag
     1021 cgcgatgtac tgccagagcg cggtgatctg cgtcgccgcg aggatcgcgg ccgcgagcat
     1081 ggccatcgcc agcgcgatca tccgcttgac gccgagccgc tcggcgatcg cggcgcagaa
     1141 cgggcccatg agtccgtaca gcagcaggtt gatcgacccc gccagggcga tcgtcgcgcg
     1201 cgacgcgccg agctcgtgct cgagcggcag catcagcacc ccgggggtcg cgcggatcgc
     1261 ggcgccgacg agcagcacga cgaaggtcgc cccggcgacg gtccacgcag ggtgcagtcg
     1321 gctcggcatc accgtcatgt atagccaggt tcgccggtcc gcgccgatac agatcgccgg
     1381 cgatcgcgcg ggcgcagcgg ccggcgccgg cgcgcggcta gccgcggtag gtgtcgtcct
     1441 gctcgatctt ggaaaccacc cagtcgaact cgccggaggt gacgtgggcg ccgcagtgct
     1501 cgcactcgcc ggactggttg atctggagcg gggcaccgca gtgactgcac gccggctggg
     1561 tgcgcggcgc gcccttgtgg ccggcgctgc ggatcagggt ccagtactcg ctgtacggcc
     1621 gctcgcggtg cttggagccg cggaccagcg cgccggtctg ggtgcggatc acgtggtcct
     1681 tgccggtcgc ccacagccgg atcgtgatcg cgtcgtagta gcgatcgcgg accagcttgg
     1741 cgagcgcggt gcgggtgatc cgcatgtcga cgagcacgtt gcgcaggccc tgctcccggt
     1801 aggcgtcgac ccagtacgac aggtagtcgt agagcccgtc ggacaccagg ccgcggaccg
     1861 ggtggaggtc gttgttcgac cacgcggcgt tgagctcgcg gtagatcatc gcgagccgcg
     1921 catcgagcgc ggcgtcggtg acctcggggt cgtcgccggt cagcgcgcgc caggcggcgt
     1981 cgacgccggg ctcgcgatag gtcggcaggt cggtgccgcg ctcggggacc tcggcggtca
     2041 gggtcggcgg ccgctcgtcg gacgagctca gcgcgacgtc ctcgacgatc cagtcgaacc
     2101 ggccgttgtc gaccgcctgg ccgcacgacg cgcagacctg cgtggtggtg cgcgacgcct
     2161 gccacggcgc gccgcagtgg gggcacggga acgtgcgggt cgcgccgggc ggcttgctct
     2221 gcaccgtggc gtcgcggccg aacacccagg tctcgacggt gtaggacgtg tggtcggtgg
     2281 tggcgaggtt ggcctcgtac tcgacctgga cccgcacgcg gcgcggccgg ccgtcgcggc
     2341 cggtcggggc gtcggggagc tcgacgcgga tcacgcgcag cgcgccgacc acgacctgga
     2401 ccaaaggctg gcccgccggc ccgtgctcgg ccagctcggc gcgcgcgtcg gccgagacgt
     2461 agggcgcgac ggtggcgagc gcctcggcgg tgtggcgcgc ccggtgcgcg gtcgagaaca
     2521 gccggaacgc gaagtcctcg aacacgacct gggagaacgc cggatcgagc cgccgcacgt
     2581 cggacagctc gatcgcgtcg tgcagcgtgg ccggcggccc gctgtcccag tccttgttct
     2641 gctgctggcg gtaggcgctg acgatcacgt agccgacgat cagcccgacg atcggcaggc
     2701 cgatcgccgg gtacgcgaag atcaggcgga acagccagta caccagctcg aagctcacgc
     2761 cgccgccgcc gcccccgccg ccgctccccg agtggccacc gccgcccgaa tagctctcgc
     2821 cgccgcccgg ccgcgccgct gcgatcgcct cgag
//
LOCUS       DASVOR010002382         2371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_230745,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002382 DASVOR010000000
VERSION     DASVOR010002382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230745"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="VF516_43880"
     CDS             complement(<1..583)
                     /locus_tag="VF516_43880"
                     /inference="COORDINATES: protein motif:HMM:NF013488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HEX8114772.1"
                     /translation="MAKLRIDIWSDIACPWCYVGKRRLEQALARFSHRDDVEVVWRAF
                     ELDPSAPRVRDTRQSYAERLARKYGTTPAQAQAMIDRMVDTAAGDGLALRFDHIRPGN
                     TFDAHRLLHLAHERGVQDALKERLLRAYLTEGQVIGEPDVLAPLAREVGLDEREVADL
                     LAGDRCAAEVRQDEALARELGITGVPFFVLAGRL"
     gene            complement(633..1019)
                     /locus_tag="VF516_43885"
     CDS             complement(633..1019)
                     /locus_tag="VF516_43885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114773.1"
                     /translation="MRHDRIDPAQNTTVPRDEDWQPDVIGKAHVDTGDAGAVVRERTE
                     RSAGDPWLDLKSRFVDDPAGAVADAEQLVRQAVDERIRELQDEAAAVFAHDDDDSNGD
                     ASTEGLRNRLIRYQAYCQRLAGRALH"
     gene            complement(1009..1584)
                     /locus_tag="VF516_43890"
     CDS             complement(1009..1584)
                     /locus_tag="VF516_43890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114774.1"
                     /translation="MNDVIWLIAVAVVVLAIAAAAFAAQRMRSRRLRTHFGPEYDHAM
                     EGFGQRRAAERALIERARRVDKLEIRPLPPLRRDELFARWQAVQAQFVDDPDGAVREA
                     HALLVTVMREQGYPTGRFDEEVELLSVHHPAAVQHYREAHRLADDRARGRSATEELRQ
                     AMIHYRALFDDLLHLGVARTQAPRREVRHEA"
     gene            complement(1948..>2371)
                     /locus_tag="VF516_43895"
     CDS             complement(1948..>2371)
                     /locus_tag="VF516_43895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114775.1"
                     /translation="FAWQQFSQSVAELPVGNIVKSGCHVAPPPEVIAAYDAPFPDERY
                     KAGARQFPLLVPTRPDDPAAAANRAAWQVLERWDKPFLTAFSDRDPITRGADRVLQQR
                     IPGAAGRVHPTVEGAGHFLQEDRGEELARIVIDFARGG"
BASE COUNT          311 a          863 c          878 g          319 t
ORIGIN      
        1 cgagccggcc ggcgaggacg aagaacggca cgccggtgat gccgagctcg cgggcgagcg
       61 cctcgtcctg ccggacctcc gcggcgcagc ggtcgccggc gagcaggtcg gcgacctcgc
      121 gctcgtcgag gccgacctcg cgggcgagcg gggcaagcac gtcgggctcg ccgatcacct
      181 ggccctcggt caggtaggcg cgcagcaggc gctccttgag cgcgtcctgc acgccgcgct
      241 cgtgggcgag atggagcagg cggtgggcgt cgaacgtgtt gcccggccgg atgtgatcga
      301 agcgcagcgc gaggccgtcg ccggccgcgg tgtcgaccat gcgatcgatc atcgcctggg
      361 cctgcgccgg tgtcgtgccg tacttgcgag cgagccgctc ggcgtagctc tgccgggtgt
      421 cgcgcacgcg cggcgccgac ggatcgagct cgaacgcgcg ccacaccacc tcgacgtcgt
      481 cgcggtgaga gaaccgggcg agggcctgct ccaggcggcg cttgccgaca tagcaccacg
      541 ggcacgcgat gtcggaccag atgtcgatgc ggagcttcgc cacgccgcca ccataccccg
      601 gagccggcgc cgcgggcagg gggccgcggg ggtcaatgca gcgccctgcc ggcgaggcgc
      661 tggcagtacg cctggtagcg gatcagccgg ttgcgcagcc cctcggtcga cgcgtcgccg
      721 ttcgagtcgt cgtcgtcgtg cgcgaagacc gcggcggcct cgtcctggag ctcgcggatc
      781 cgctcgtcga ccgcctgccg cacgagctgc tcggcgtccg cgaccgcgcc ggccggatcg
      841 tcgacgaacc ggctcttgag atcgagccac ggatctccgg cggatcgctc ggtgcgctcg
      901 cggacgaccg cgccggcgtc gccggtgtcg acgtgcgcct tgccgatcac gtcgggctgc
      961 cagtcctcgt cgcgcggcac ggtggtgttc tgggctggat cgatccggtc atgcctcatg
     1021 gcgaacctct cggcgggggg cttgcgtgcg cgcgacgccc aggtggagca ggtcgtcgaa
     1081 cagcgcgcgg tagtggatca tggcctggcg aagctcttcg gtcgcgctgc gcccgcgggc
     1141 gcggtcatcg gccaggcggt gggcttcccg gtagtgctgc accgccgcgg ggtggtgcac
     1201 cgagagtagc tcgacctcct cgtcgaaccg tccggtcgga tagccctgct cgcgcatgac
     1261 ggtgaccagc agcgcgtggg cctcgcggac cgcgccgtcc ggatcgtcga cgaactgggc
     1321 ctggaccgcc tgccagcgcg cgaacagctc gtcgcggcgc agcggcggga gcggccggat
     1381 ctcgagcttg tcgacgcgcc gggcccgctc gatcagcgcg cgctccgcgg cccggcgctg
     1441 gccgaagccc tccatcgcat ggtcgtactc cggcccgaag tgggtccgca gccggcggct
     1501 gcgcatccgc tgtgcggcga acgccgcggc tgcgatcgcc agcaccacga cggcgacggc
     1561 gatcagccag atcacatcgt tcatagcgtt cctccttatg ccaacatgcc aatcaccgac
     1621 agcacgaaac gtgccgtcgc cgccgggtgc gaagatctgg tgtgcgcccc gcgccgcgcg
     1681 tcgccgcgtc gagcgcggca ccggaaccgt cgagaatcgc gctggaccgc cgatgcgaga
     1741 tcgcccgggc cgcgcacggc ctcgatcgcg atgcgcgtgc gcgcctcacc gccgccgcga
     1801 gcccgggaaa ttctcgggct acggaaacgc gacctcgacc gcccgcatcg ttgcccgccc
     1861 gcgcggcggg atcaacgacc gcccgccaac acgccatctc agcccgtgcc cccgcccccg
     1921 ccgtgcaccc gcggcgagag gtacgaccta gccacctcgt gcgaagtcga tcacgatgcg
     1981 cgccagctcc tcgccgcggt cctcctgcag gaagtgcccg gcgccctcga ccgtgggatg
     2041 gacgcggccg gcggcgcccg ggatccgctg ctgcagcacc cggtcggcgc cgcgggtgat
     2101 ggggtcgcgg tcggagaacg cggtgagaaa cggcttgtcc cagcgctcca gcacctgcca
     2161 tgccgcgcgg ttggccgcgg ccgccgggtc gtcgggccgg gtcggcacga gcagcgggaa
     2221 ctggcgcgcg ccggccttgt agcgctcgtc ggggaacggc gcgtcgtagg cggcgatcac
     2281 ctcgggcggc ggggcgacgt ggcagccgga cttgacgatg ttgccgaccg gcagctcggc
     2341 cacgctctgg gagaactgct gccacgcgaa g
//
LOCUS       DASVOR010002383         4068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_318465,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002383 DASVOR010000000
VERSION     DASVOR010002383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318465"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1189)
                     /locus_tag="VF516_43900"
     CDS             complement(<1..1189)
                     /locus_tag="VF516_43900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114776.1"
                     /translation="MTRTLGILAIAAGAAAARPAHADDTAIARPADASRAGDDAIDLR
                     LTLSTFLYRESGADAPALVDQGATLPNASPVRRTFGDLRTELAAGGLTLDARIRQTTS
                     DRYQAGAAGGGEYELRTLDYRLGTLATSLTIGRQVIDAVGATRIDGAALSHRLTGGLS
                     ATVFGGAYPALGSRSLDTDYVHAPSPGGPSGGDGALVVPVTGGLGVAYQAPDYHGDLG
                     IAAVRVVQDVPDATAAERSRVFTTSSGYWRFGAPLDAYHFAVLDLAGAGGVHLTNGSV
                     GIDARPGAGVQLSLALHHVSTDVLQIAARNLLSDPDPSAIGIVQNNLAVTRISQDVAR
                     AGASIALAAQRFELSASGGLHRRPEVSVALADGSGAVVFPEARSADATLSILDRRSLG
                     GLRV"
     gene            complement(1186..3516)
                     /locus_tag="VF516_43905"
     CDS             complement(1186..3516)
                     /locus_tag="VF516_43905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114777.1"
                     /translation="MTTTRSPVVLLAALALALVGWGAGTARADDFLKSSPGELAASHA
                     AIDHQDQCASCHEPDNTVSAAKCLGCHDHQDLNKRIDANEGFHASARVKNRPCKVCHQ
                     EHRGRGFDLMGWNAIGGPKAFDHKLAGWPLAGKHAVTDCTGCHKTVNPQGLRTYLHTE
                     RACASCHTKEQPHGPVRPVNLRCERCHGESQWKPQKPALELDHNSPGQAAMPLVGTHA
                     DVACAKCHPRAVFKLAKFEGSCAQCHQSPHDGQLFGTRKCELCHSPALRSMDEVQFDH
                     KKQTGYALIARHAQIGCEGCHTKALGKQKPAAACETCHAKDNKHAGRFDKVSTACATC
                     HSQRAWTTAFQFNHQEKTGFDLTGKHAKAACRDCHRGKSPSEFERFDIKNGCMSCHKH
                     GKAHGGKFKNDQCLTCHAEAGNRKMRTDTAAGSARETFHGEGSKFPLRGGHATVQCQM
                     CHVNDVYQETPRECGVSCHEDSLHKGSLGQACSRCHEPGQWPAVRFDHRTQTRWPLEG
                     KHTQVKACEACHPSRRYHGTPTACAASGCHKADDVHQGKLGNQCETCHATDGTLVFRH
                     NRDAQFKIDGKHAPLVCAACHKSVAFKPVRRDCFGCHPEPAIHKGRYGTDCQRCHSTR
                     SFGDVRAQHDVGDFSLTGAHDQLPCARCHPTGEKLRGSGNLCISCHKRDDIHGNTLSP
                     RCGECHTQRAFAPARFDHVTVGCNLMGLHGTLPCADCHKAGNYGAVSPMCVSCHRGDA
                     LRVKTPDHRTLVECGGCHNPSAWIPATQLGQQSICR"
     gene            complement(3519..>4068)
                     /locus_tag="VF516_43910"
     CDS             complement(3519..>4068)
                     /locus_tag="VF516_43910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114778.1"
                     /translation="ARVSASDSSPGLDYPGPTAPPGKIPFRTFETDAGVRLGAQLQRA
                     FNVALRAGVHYDAFVTRSVANAGMLPRERLLGATFGARVDIAPGRSRFAVTARFDALV
                     LGGRAQTAGLQDGTSSTAHALWGGLTLRYVLGHRLAAFGGYDFGRATTEWSGMSARQP
                     GVTRTRRVDTAQLVQIGISAEL"
BASE COUNT          497 a         1427 c         1537 g          607 t
ORIGIN      
        1 gcacgcgcag cccgccgagc gagcgccggt cgaggatcga cagcgtcgcg tcggccgacc
       61 gcgcctcggg gaacaccacc gcacccgagc cgtcggcgag cgcgaccgag acctcgggac
      121 ggcgatgcag cccgccggac gccgacagct cgaagcgctg cgcggcgagc gcgatgctgg
      181 cgcccgcgcg cgccacgtcc tgcgagatcc gggtcaccgc gaggttgttc tggacgatgc
      241 cgatcgccga cgggtccgga tcggacagca ggttgcgcgc cgcgatctgc aggacgtcgg
      301 tcgacacgtg gtggagcgcc aggctgagct ggacgcccgc gcccgggcgc gcgtcgatcc
      361 cgacgctgcc gttggtcagg tggacgccgc cggccccggc gaggtcgagc accgcgaagt
      421 ggtaggcgtc gagcggcgcg ccgaaccgcc agtagccgct cgacgtcgtg aacacgcgcg
      481 accgctcggc ggcggtcgcg tcgggcacgt cctggaccac gcggaccgcc gcgatcccga
      541 ggtcgccgtg gtagtccggc gcctggtagg ccacgccgag cccgccggtg acgggcacca
      601 ccagcgcccc atctccgcca ctcgggccgc ccgggctcgg ggcgtggacg tagtcggtgt
      661 cgagcgaccg cgagccgagc gcgggatagg cgccgccgaa caccgtcgcg gagagcccgc
      721 cggtcagccg gtggctcagc gcggccccgt cgatccgcgt cgcgccgacc gcgtcgatca
      781 cctggcgccc gatcgtcagc gacgtcgcca gcgtgccgag ccggtagtcg agcgtgcgca
      841 gctcgtactc accgccgccc gccgcgccgg cctggtagcg atcgctggtg gtctggcgga
      901 tccgcgcgtc gagcgtcagc ccgccggccg cgagctcggt gcgcagatcg ccgaacgtgc
      961 ggcgcaccgg gctggcgttc ggaagggtcg cgccctgatc gaccagcgcg ggggcgtcgg
     1021 cgccggactc gcggtacaga aacgtcgaca gcgtgagccg caggtcgatc gcatcgtcgc
     1081 cggcgcggct cgcgtcggcc gggcgcgcga tcgcggtgtc gtcggcgtgc gccggccgcg
     1141 ccgccgcggc gccggccgcg atcgccagga tgccgagcgt gcgggtcacc tgcatatcga
     1201 ttgctgtccg agctgcgtgg ccgggatcca cgccgagggg ttgtggcagc cgccgcactc
     1261 gaccagcgtc cggtggtcgg gcgtcttgac gcgcagcgcg tcgccgcggt ggcagctgac
     1321 gcacatcggc gacaccgcgc cgtagttgcc ggccttgtgg cagtcggcgc acggcagcgt
     1381 gccgtgcagc cccatcaggt tgcagccgac cgtgacgtga tcgaaccgcg ccggggcgaa
     1441 cgcgcgctgg gtgtggcact cgccgcagcg cggcgacagc gtgttgccgt ggatgtcatc
     1501 ccgcttgtgg cacgagatgc acaggttgcc cgagccgcgc agcttctcgc cggtcgggtg
     1561 gcagcgcgcg cacggcagct gatcgtgggc gccggtcagc gagaagtcgc cgacgtcgtg
     1621 ctgggcgcgg acgtcgccga acgaccgggt cgagtggcaa cgctggcagt cggtgccgta
     1681 gcggcccttg tggatcgccg gctcggggtg gcagccgaag cagtcgcggc gcaccggctt
     1741 gaacgcgacc gacttgtggc acgccgcgca caccagcggc gcgtgcttgc cgtcgatctt
     1801 gaactgcgcg tcgcggttgt ggcggaacac cagcgtcccg tcggtcgcgt ggcaggtctc
     1861 gcactggttg ccgagcttgc cctggtgcac gtcgtcggcc ttgtggcagc ccgacgcggc
     1921 gcacgcggtc ggcgtgccgt ggtagcgccg gctcgggtgg caggcctcgc acgccttgac
     1981 ctgggtgtgc ttgccctcga gcggccaccg ggtctgggtc cggtggtcga agcgcaccgc
     2041 gggccactgg ccgggctcgt ggcagcgcga gcacgcctgg ccgagcgagc ccttgtgcag
     2101 cgagtcctcg tggcacgaca ccccgcactc gcgcggggtc tcctggtaca cgtcgttgac
     2161 gtggcacatc tggcactgca cggtggcgtg gccgccgcgc agcgggaact tcgagccctc
     2221 gccgtggaag gtctcgcgcg ccgagcccgc cgcggtgtcg gtccgcatct tccggttgcc
     2281 ggcctcggcg tggcaggtca ggcactgatc gttcttgaac ttgccgccgt gcgccttgcc
     2341 gtgcttgtgg cagctcatgc agccgttctt gatgtcgaac cgctcgaact ccgacggtga
     2401 cttgccgcgg tgacagtcgc ggcaggccgc cttcgcgtgc ttgccggtca gatcgaagcc
     2461 ggtcttttcc tggtgattga actgaaaggc cgtggtccac gcgcgctgcg agtggcaggt
     2521 cgcgcacgcg gtcgacacct tgtcgaaccg gccggcgtgc ttgttgtcct tggcgtggca
     2581 ggtctcgcac gccgccgccg gcttctgctt gccgagcgcc ttggtgtggc agccctcgca
     2641 cccgatctgg gcgtgcctgg cgatcagcgc gtagccggtc tgcttcttgt gatcgaactg
     2701 gacctcgtcc atcgagcgca gcgccggcga gtggcagagc tcgcacttcc tggtgccgaa
     2761 cagctggccg tcgtgcgggc tctggtggca ctgcgcgcag ctgccttcga acttggccag
     2821 cttgaacacc gccctggggt ggcacttggc gcacgcgacg tcggcgtggg tgccgaccag
     2881 cggcatcgcg gcctggccgg gactgttgtg atcgagctcg agcgccggct tctgcggctt
     2941 ccactgcgac tcgccgtggc agcgctcgca gcgcaggttg accgggcgga ccgggccgtg
     3001 cggctgctcc ttggtgtgac agctcgcgca cgcgcgctcg gtgtggaggt aggtccgcag
     3061 gccctgcggg ttgaccgtct tgtggcaacc ggtgcagtcg gtgaccgcgt gcttgccggc
     3121 gagtggccag cccgcgagct tgtgatcgaa cgccttcggc ccgccgatcg cgttccagcc
     3181 catgaggtcg aagccgcggc cgcggtgctc ctggtggcac accttgcacg gccggttctt
     3241 gacccgggcc gatgcgtgga agccctcgtt ggcgtcgatc cgcttgttca ggtcctggtg
     3301 atcgtggcag ccgaggcact tggcggccga caccgtgttg tcgggctcgt ggcagctcgc
     3361 gcactggtcc tggtggtcga tcgcggcgtg gctcgcggcg agctcgccgg gcgaggactt
     3421 caggaagtcg tcggcgcgcg cggtgccggc gccccacccg acgagcgcga gcgccagggc
     3481 ggcgagcagc accacgggag atcgggtcgt cgtcacgctc acagctccgc gctgatcccg
     3541 atctggacca gctgggcggt gtcgacccgc cgcgtgcgag tgacgccggg ttgccgcgcc
     3601 gacatccccg accactcggt cgtggcgcgg ccgaagtcgt agccgccgaa cgccgcgagg
     3661 cggtggccga ggacgtagcg cagggtgagc ccgccccaca gcgcgtgcgc ggtgctcgac
     3721 gtcccgtcct gcagcccggc ggtctgggcg cgtccgccga gcaccagcgc atcgaaccgc
     3781 gcggtgaccg cgaaccgcga tcgcccgggc gcgatgtcga cccgggcgcc gaacgtcgcg
     3841 ccgagcaggc gctcgcgcgg cagcatcccg gcgttggcca cgctgcgagt cacgaacgcg
     3901 tcgtagtgga cgccggcgcg cagcgcgacg ttgaacgcgc gctggagttg cgcgccgagc
     3961 cgaacgccgg cgtcggtctc gaacgtgcga aacgggatct tgccgggcgg cgcggtcggg
     4021 cccggatagt cgaggccggg cgacgagtcg ctcgccgaca cccgcgcg
//
LOCUS       DASVOR010002384         2077 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_522436,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002384 DASVOR010000000
VERSION     DASVOR010002384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522436"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(142..963)
                     /locus_tag="VF516_43915"
     CDS             complement(142..963)
                     /locus_tag="VF516_43915"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114779.1"
                     /translation="MLVAAAGCGSDNKSAPDAAVPGDAAVAIDGPAAAVTLKLANSTT
                     LGNYLADSSGRALYLYSKDFPATSGAAANPAVSNCSGGCLTAWPVFHADTIAPGPGLK
                     AADFGELTRTDGTKQTTFHGWPLYYFASDTQPSDTKGEGVGNVWFVLRDPFYSMLVMS
                     SPAAGGPTLYLSTPEGMTLYYYTPDTVGTGSTPPVSACTGACLQAWPAFLATAAVLPS
                     KVAASLTSFDRGGGVMQSAWKGHPLYLFATDKKPGDVKGDKVGNIWFVVDPTTLQ"
     gene            complement(1354..>2077)
                     /locus_tag="VF516_43920"
     CDS             complement(1354..>2077)
                     /locus_tag="VF516_43920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEX8114780.1"
                     /translation="NGKVERGEECDDGNRVDGDGCSVACMTEPPPVCGNGKVERDEQC
                     DDGNRIDGDGCSAACILEPAPVCGNGKVERDEQCDDGNRVDGDGCSAACMTEPPPVCG
                     NGKVERGEGCDDGNTVNGDGCTSKCQLEVHLALPAGLRSQGLQPPGDEWRSDKINVRP
                     SRETKELMIRANVLSVTGVVRLCIGVAGEVTEAEITKSTGYDRYDQRLVHAVRSWTYR
                     PHRVHDRAVPACGQVVFHYEIK"
BASE COUNT          313 a          742 c          657 g          365 t
ORIGIN      
        1 ctcgcggtgc tcggcgtgcc cggcggcgtg ctctcggccg tgcccgtggt gtggcggttc
       61 gtccgcgacg tgctgcgcga gctccgccag cgcgacgcct cgctccgcga ggccagatga
      121 tccggcccgc gcggcgctcg actattgcag cgtggtcggg tcgacgacga accagatatt
      181 ccccaccttg tcacccttga cgtcccccgg cttcttgtcg gtcgcgaaca ggtacagcgg
      241 gtggcccttc cacgccgact gcatgacgcc gccgccgcga tcgaagctcg tcagcgacgc
      301 ggcgaccttg ctcggcagca cggcggcagt ggcgaggaac gccggccagg cttgcaggca
      361 tgcgcccgtg caggcggaca ccggcggtgt cgaccccgtc cccaccgtgt ccggcgtgta
      421 gtagtacagc gtcatgccct cgggcgtcga caggtagagc gtcgggcctc ccgccgcggg
      481 gctgctcatc accagcatcg agtagaacgg atcgcgcagc acgaaccaca cgttgccgac
      541 gccctcgccc ttggtatcgc tcggctgcgt gtcgctggcg aagtagtaga gcggccagcc
      601 gtggaaggtc gtctgcttgg tgccgtcggt ccgcgtcagc tcgccgaagt cggccgcttt
      661 caggccgggc cccggagcga tcgtatcggc gtggaacacc ggccaggcgg tcaggcaccc
      721 gccggagcag ttcgagaccg ccggattcgc ggcggcgccc gacgtggccg ggaaatcctt
      781 cgaataaaga tacagcgcgc gtccgctgct atccgcgagg tagttgccga gcgtcgtgct
      841 gttggccagc ttgagggtca ccgccgcggc cgggccgtcg atcgcgaccg ctgcgtctcc
      901 cgggaccgca gcgtcgggtg cactcttgtt atcgctgcca caaccggcag cagcgacgag
      961 catcgaagtc gcaaagagct gcagagtcac acggactgac ttcaggatgg ctttcttcat
     1021 gaacaactcc tgttggcacc cgcggttcta gctccggatg cgggacacga gcaacgcgac
     1081 gcgcccgagg acgcacggcg gtgtgccgcc cgtcgcgtgt gaagttcatg aagcagcaac
     1141 accgctcgcc gtgacagcca gtgccggcag atcgctcgaa tgacaatcat gattgaatat
     1201 caatgatcga gacaggatca ctcatcctct gccgagtacg acacatcacc attcatcgag
     1261 ttctctgcga cttcttctca tgacgtgact tcagcgagtg acttccacgc atcgccatcg
     1321 tcgacgaggc ggcgatctgc cccggcgccg ccgctacttg atctcgtagt ggaagacgac
     1381 ctggccgcac gccgggacgg ctcgatcgtg gacccggtgc gggcgatagg tccagctgcg
     1441 gaccgcatgg accagccgct gatcgtagcg atcgtatccg gtcgacttcg tgatctcggc
     1501 ttccgtcacc tcgcccgcga cgccgatgca cagccggacg acgccggtca ccgagagcac
     1561 gttggcgcgg atcatcagct ccttggtctc cctgctcggg cgtacgttga tcttgtcgga
     1621 tctccattca tcgccgggcg gttgcagacc ctgcgatcgc agacccgctg gcaatgcgag
     1681 gtggacctcg agctggcact tcgatgtgca gccatcgccg ttgacggtgt tgccgtcgtc
     1741 gcacccctcg ccgcgctcca ccttgccgtt gccgcacacc ggcggcggct ccgtcatgca
     1801 cgccgccgag cagccgtcgc cgtcgacccg gttgccgtcg tcgcactgct cgtcgcgctc
     1861 caccttcccg ttgccgcaca ccggcgccgg ctcgaggatg cacgccgccg agcaaccatc
     1921 gccgtcgatc cggttgccgt catcgcactg ctcgtcgcgc tccaccttcc cgttgccgca
     1981 caccggcggc ggctccgtca tgcacgccac cgagcagccg tcgccgtcga cccggttgcc
     2041 gtcgtcgcac tcctcgccgc gctccacctt cccgttg
//
LOCUS       DASVOR010002385         4698 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_376828,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002385 DASVOR010000000
VERSION     DASVOR010002385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376828"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..777
                     /locus_tag="VF516_43925"
     CDS             49..777
                     /locus_tag="VF516_43925"
                     /inference="COORDINATES: protein motif:HMM:NF017916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEX8114781.1"
                     /translation="MINYWPLLGVAVVVAGFALRYNPMLVVVTAGLVSGLAAGKSPGE
                     LLALLGTTLVSNRTLLLFAVTFPAIGVLERAGLRERASAWILGFARLTLARLLIAYLA
                     ARQLLAMVGLIYVAGHPQTVRPLLAPMTEATATRRLGVLDPASRERVRALAAATDNVG
                     VFFGEDVFLAFGAVLLIQGFYDGQGIHLDPLSIGLWALPTAIAAFVIHAVRILIFERR
                     LARTLPEARLVGEPPAVPPPGEAG"
     gene            778..1749
                     /locus_tag="VF516_43930"
     CDS             778..1749
                     /locus_tag="VF516_43930"
                     /inference="COORDINATES: protein motif:HMM:NF017931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 family protein"
                     /protein_id="HEX8114782.1"
                     /translation="MLTLTHVYYAIGATLVGAAVLNLRDRRWLQAAFWAILAASCLVG
                     DSILAANKAGTRWPAQVMGAGVIALALLAWRIRARKVVDDPEQARAREASAARLGNRL
                     FLPALAIPGITLVLVLGARIDALRITAWIDPKQLPLISLGLAATLALVLGIALTRARP
                     VHGLTEGRRLLDSIGWPLALPLLLATLGGVFVETGVGKAISALVGDVIPTDSAAACLL
                     AFGLGMVAFTAIMGNAFAAFPVMMAGIGLPLLVKVHGAQPAVLGAMGMLTGYCGTLIT
                     PMAANFNVVPVLLLELRDPHAVIRAQWPTALALLIANLILMGVLVFR"
     gene            1752..4409
                     /gene="mgtA"
                     /locus_tag="VF516_43935"
     CDS             1752..4409
                     /gene="mgtA"
                     /locus_tag="VF516_43935"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR01524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEX8114783.1"
                     /translation="MHAALPKRLLAGSHRDRPPIQVSPLLREIAALDPEPALARMASR
                     AGGLTTGEAAARLAEHGANQVARDDRPGFVRLLGRSVVNPLVLLLAVLAAVSLATGDS
                     RAATMMIAMIVLGVGLKLVQEARADSAAARLKAMITVHATALRDGQPCEVPLAQLVPG
                     DVVQLTAGDMIPADVRIVSAKDLFVTQGALTGESFPVEKHAVDPSAAGAAPLDATTIA
                     FLGTSVESGAASAVVVATGRHTYLGGMAASMAEPGAPTAFDRDISRFTWLLLRFMFVM
                     VPLVFVINGAAKGRWDQAFFFALAVAVGLTPEMLPMIVTVCLSRGAVAMARRKVIVKR
                     INAIQNLGAMDVLCTDKTGTLTQDRVILQRHCDVALRDSDDVMALAYLNSHFQTGLRN
                     VLDRAVLAREDGHARARVAEVSKVDEIPFDFQRRIMSVVVRTPDGQDLLVAKGAPEAI
                     FERCARFALDGRELAIDPAAVGELQREYERLAGDGFRVLAIATRRSAPRSAAAGATAY
                     GKADEHDLVLQGYVAFLDPPKDTCTAAIARLQARGITVKVITGDSDLVARKVCKDVGL
                     AIDRVVLGADVERMTDAELAEAAEAAALFARVSPAHKQRILRALQARGHTVGFMGDGI
                     NDAPALHAADVSISVDTAVDIAKASADLVLLEKSLVVVDDGVVLGRKVFANILKYVRM
                     GASSNFGNMFSVLGASLLVPFLPMLPIQILSNNLLYDLGQTTIPTDDVDPEQVARPRP
                     WNIRALGRFIALIGPCSSVFDYTTYFVMLYVFGCRDVSTPAAAAHSASLFQTGWFVES
                     LLTQTLVIHVIRTDRLPFVHSRASRPVLAMGLAIMAIGVWLPGSPIGGSLGFTPLPAL
                     YWPILAATLVCYLALTQAVKTLLLRRGWL"
BASE COUNT          575 a         1764 c         1649 g          710 t
ORIGIN      
        1 gcacctgcgg tgtaggcaac cgccgcccgg accggctatc gtgcccgggt gatcaactac
       61 tggccgctgc tcggcgtcgc ggtggtcgtc gccggcttcg ccctccggta caacccgatg
      121 ctggtggtcg tgaccgcggg gctggtcagc gggctggcgg cgggcaagtc gcccggcgag
      181 ctgctcgcgc tgctcggcac cacgctggtc agcaaccgca cgctcctgct gttcgcggtg
      241 acgttcccgg cgatcggcgt gctggagcgc gccggcctgc gcgagcgggc gagcgcgtgg
      301 atcctcgggt tcgcccggct cacccttgcc cgcctgctga tcgcctacct ggccgcgcgc
      361 cagctgctcg ccatggtcgg cctgatctac gtcgcggggc atccccagac cgtgcggccg
      421 ctgctcgcgc cgatgaccga ggcgacggcg acgcgcaggc tcggcgtcct cgatccggcc
      481 tcgcgcgagc gcgtgcgcgc gctggccgcc gcgaccgaca acgtcggggt gttcttcggc
      541 gaggacgtgt tcctcgcctt cggcgccgtg ctgctgatcc agggcttcta cgacggccag
      601 gggatccacc tcgacccgct gtcgatcggg ctgtgggcgc tgccgaccgc gatcgcggcg
      661 ttcgtgatcc acgcggtccg catcctgatc ttcgagcgcc ggctcgcccg cacgctgccc
      721 gaggcgcggc tcgtcggcga gccaccggcg gtgccgccgc ccggggaggc cggctagatg
      781 ctgaccctga cccacgtgta ctacgcgatc ggcgcgacgc tggtcggcgc ggcggtgctc
      841 aacctccgcg accgccggtg gctgcaggct gcgttctggg cgatcctcgc cgcgtcgtgt
      901 ctggtcggtg actcgatcct cgcggcgaac aaggccggca cgcggtggcc ggcgcaggtc
      961 atgggcgcgg gcgtgatcgc gctcgcgctg ctcgcgtggc ggatccgggc gcgcaaggtg
     1021 gtcgatgatc ccgagcaggc ccgcgcccgg gaagcctcgg ccgcgcggct cggcaaccgg
     1081 ctgttcctgc cggcgctcgc catccccggg atcacgctcg tcctggtgct cggtgcccgg
     1141 atcgacgcgc tgcggatcac ggcgtggatc gatcccaagc agctgccgct gatctcgctc
     1201 ggtctcgcgg cgacgctcgc gctggtgctc ggcatcgcgc tgacccgcgc gcgtccggtg
     1261 cacggactga ccgagggccg ccggttgctc gactcgatcg gctggccgct cgcgctgccg
     1321 ctcttgctcg ccaccctggg cggcgtgttc gtggagaccg gggtcggcaa ggcgatcagc
     1381 gcgctggtgg gcgacgtgat cccgaccgac agcgccgccg cctgcctgct ggcgttcggc
     1441 ctcggcatgg tggcgttcac cgcgatcatg ggcaacgcgt tcgccgcgtt ccccgtgatg
     1501 atggccggga tcgggctgcc gctgctggtc aaggtgcacg gcgcccagcc cgccgtgctc
     1561 ggcgcgatgg gcatgctgac cggctactgc ggcacgctga tcacgccgat ggcggcgaac
     1621 ttcaacgtcg tcccggtgct gctgctcgag ctgcgcgatc cgcacgcggt gatccgcgcg
     1681 cagtggccga ccgcgctggc gctgctgatc gccaacctga tcctgatggg cgtcctcgtg
     1741 ttccgctgac gatgcacgcc gccctgccga agcggctgct cgccggcagc caccgcgacc
     1801 gcccgccgat ccaggtgtcg ccgctgctcc gcgagatcgc cgcgctcgat ccggagccgg
     1861 cgctcgcccg gatggcgagc cgggccggcg ggctgaccac cggcgaggcg gcggcgcggc
     1921 tcgccgagca cggcgccaac caggtcgcgc gcgacgatcg ccccgggttc gtccggctgc
     1981 tcggccgctc ggtggtcaac ccgctggtcc tcctgctcgc cgtgctcgcc gcggtgtcgc
     2041 tggcgaccgg cgactcgcgc gccgcgacga tgatgatcgc gatgatcgtg ctcggcgtcg
     2101 ggctcaagct ggtccaggag gcgcgggccg acagcgccgc cgcccggctc aaggcgatga
     2161 tcacggtcca cgccaccgcg ctgcgcgacg gccagccgtg cgaggtcccg ctggcgcagc
     2221 tggtccccgg cgacgtcgtg cagctgaccg cgggcgacat gatcccggcc gacgtccgga
     2281 tcgtctcggc caaggacctg ttcgtcaccc agggcgcgct gaccggcgag agctttccgg
     2341 tcgagaagca cgcggtggac ccctcggccg cgggcgcggc gccgctcgac gccacgacga
     2401 tcgcgttcct cggcaccagc gtcgagagcg gggccgcctc ggcggtggtc gtcgcgaccg
     2461 gccgccacac ctacctcggc ggcatggcgg cgtcgatggc cgagcccggc gcgcccaccg
     2521 cgttcgatcg cgacatctcg cggttcacct ggctgctgtt gcgcttcatg ttcgtgatgg
     2581 tcccgctggt gttcgtgatc aacggcgccg ccaagggccg ctgggaccag gcgttcttct
     2641 tcgcgctcgc ggtcgcggtc ggcctgacgc ccgagatgct gccgatgatc gtgacggtgt
     2701 gcctgtcgcg gggcgcggtc gcgatggccc ggcgcaaggt gatcgtcaag cggatcaacg
     2761 cgatccagaa cctcggcgcg atggacgtgc tgtgcaccga caagaccggc acgctgaccc
     2821 aggaccgcgt gatcctccag cgccactgcg acgtcgcgct gcgcgacagc gacgacgtga
     2881 tggcgctcgc ctacctcaac agccacttcc agaccggcct gcgcaacgtg ctcgaccgcg
     2941 cggtgctcgc ccgcgaggac ggccacgccc gcgcccgggt cgccgaggtc agcaaggtcg
     3001 acgagatccc gttcgacttc cagcgccgga tcatgtcggt cgtcgtccgc acaccggacg
     3061 gccaggacct gctggtcgcc aagggcgcac ccgaggcgat cttcgagcgc tgcgcgcggt
     3121 tcgcgctcga cggccgggag ctcgcgatcg accccgccgc ggtcggtgag ctccagcgcg
     3181 agtacgagcg gctcgccggc gacggcttcc gcgtgctggc gatcgcgacc cggcgtagcg
     3241 ccccgcgcag cgcggccgcc ggcgccaccg cctacggcaa ggccgacgag cacgacctcg
     3301 tgctccaggg gtacgtcgcg ttcctcgatc cgcccaagga cacctgcacc gcggcgatcg
     3361 cccggctcca ggcccgcggc atcaccgtca aggtgatcac cggcgacagc gatctggtcg
     3421 cccgcaaggt gtgcaaggac gtcgggctcg cgatcgaccg cgtcgtgctc ggcgccgacg
     3481 tcgagcgcat gaccgacgcc gagctcgccg aggccgccga ggccgccgcg ctgttcgccc
     3541 gggtgtcgcc ggcccacaag cagcgcatcc tgcgggcgct gcaggcccgc ggccacaccg
     3601 tcgggttcat gggcgacggc atcaacgacg cccccgcgct gcacgccgcc gacgtcagca
     3661 tcagcgtcga caccgcggtc gacatcgcca aggcgtccgc cgatctcgtc ctgctcgaga
     3721 agtcgctggt cgtggtcgac gacggcgtgg tgctcggccg caaggtgttc gccaacatcc
     3781 tcaagtacgt gcggatgggc gcgtcgagca acttcggcaa catgttcagc gtgctcggcg
     3841 cgagcctgct cgtgccgttc ctcccgatgc tgccgatcca gatcctcagc aacaacctgc
     3901 tctacgacct cggccagacc acgatcccga ccgacgacgt cgaccccgag caggtcgccc
     3961 ggccgcggcc gtggaacatc cgcgcgctcg gccggttcat cgcgctgatc ggcccgtgct
     4021 cgtcggtgtt cgactacacg acctacttcg tcatgctcta cgtgttcggc tgtcgcgacg
     4081 tgtcgacccc ggccgccgcg gcgcacagcg cgagcctgtt ccagaccggc tggttcgtcg
     4141 agagcctgct gacccagacc ctggtgatcc acgtgatccg caccgaccgg ctgccgttcg
     4201 tgcacagccg cgcgtcgcgg ccggtgctcg ccatgggcct ggcgatcatg gcgatcggcg
     4261 tgtggctgcc gggatcgccg atcggcggat cgctcgggtt cacgccgctg cccgcgctgt
     4321 actggccgat cctcgccgcc acgctggtgt gctacctcgc cctgacccag gcggtgaaga
     4381 ccctgctgct gcgccgcggc tggctgtagc ccgcgccgcg cggcccacgg cgagccgccg
     4441 atcagcggaa tcccgatcgg cgccgatccg cgcgagggca ccggaattgc gtccccagca
     4501 accatggtga tccgttgcaa caggagtttc cccatgcgtc ttccgacgag gtcgtcgatc
     4561 aggacctcga tcctggtcgg cggctgcatc gtggcggcca ccgtcgcctg ccaccggaac
     4621 gagcagccgc acgagcgcgc ctcgccgccg caggtgaccg cgccacagcc gcgaccggcg
     4681 ccgcccccca cgccggcc
//
LOCUS       DASVOR010002386         2154 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_522465,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002386 DASVOR010000000
VERSION     DASVOR010002386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2154
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522465"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(437..724)
                     /locus_tag="VF516_43940"
     CDS             complement(437..724)
                     /locus_tag="VF516_43940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114784.1"
                     /translation="MTGPAGPGHRHARGRLGHPGFELKPLVIEIGEVPRIVDPVEVQK
                     LPELGVLSAMANPDLEVVIAAMSAVPGLPEDRARLYLDVIWRATPPPLRKC"
     gene            complement(721..1308)
                     /locus_tag="VF516_43945"
     CDS             complement(721..1308)
                     /locus_tag="VF516_43945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114785.1"
                     /translation="MSTPVTRGELQDVLSQVKQEHWRDLREATAPLATRAELQAAIAP
                     LATTAELQAAVAPLATKAELQELRNELHAAVAPLATKAELEMWGGALVARIQHGEQRT
                     GNLERGQEQLIELAQQTQQQIQQTEQRFSDHLQQTEQRLLAELARHTSAVHEAMRTEI
                     KASFEPLADLTGRMSRLEAAVFEPKPDRPTRRRKH"
BASE COUNT          311 a          726 c          751 g          366 t
ORIGIN      
        1 caacggcacg ccgacccagc cggtcgccgc gggctacatg gccaacgcgt ggctgcgcat
       61 gaagcacgag aactacgacg agctgcgccg catcctcgac atcgtcggcc agaccgtcaa
      121 ggtccacgcc cgctgacgcc cagccaagct ggccgggggc tcgccgcctg tcgccctcaa
      181 cgcggcgtgc gcgcggccac cactgcgccc cggatctcgg cagcgctgga cgcgcgcacg
      241 agcgtaccga tcgaatcggg tcagtgactt ctcgtcgatg acctcgtcga tggccgctcg
      301 tcctcgatgg aaacggtgcc gagcttcgcg cgcaccagtg cgagcacccc aaggcgcagg
      361 ccatcttcgc gcgcctgctg ggcaagaagg cgatccactt cggccctcga tcgctcgtac
      421 acctgagctt ccgtgttcag cactttcgca ggggcggcgg agtcgccctc cagatcacgt
      481 ccaagtatag ccgggctcga tcctccggca gcccgggaac ggcagacatc gccgcgatca
      541 caacctcgag atccgggttg gccatcgccg acaggactcc gagctccggc agcttctgga
      601 cctccaccgg atcgacaatg cgcggcacct cgccgatctc gatcaccagc ggcttgagct
      661 cgaatccggg atggccgagc cgaccgcgcg cgtgtcggtg gcccggcccg gccgggccgg
      721 tcaatgcttt cgtcgtcggg tcggccggtc gggcttgggc tcgaacaccg cggcctcgag
      781 ccggctcatc cgcccagtca gatcggcgag cggttcgaag ctcgccttga tctcggtccg
      841 catcgcttcg tggaccgccg aggtatggcg tgcgagctct gcgagcaatc gctgctcggt
      901 ctgctgtaga tgatccgaga accgctgctc ggtctgctgg atctgttgct gggtctgctg
      961 ggcgagctcg atcagctgct cctgcccgcg ctccaggttg ccggtgcgct gctcgccgtg
     1021 ctggatgcgt gccaccaggg ctccacccca catctccagc tcggccttgg tggcgagcgg
     1081 agcgacggcg gcatggagct cgttccggag ctcctggagc tcggccttgg tggcgagcgg
     1141 agcgacggcg gcctggagct cggccgtggt ggcgagtggg gcgatggcgg cctggagctc
     1201 ggccctggtg gcgagcgggg cggtggcctc gcgaaggtct cgccagtgct cctgcttgac
     1261 ctgcgagagg acgtcttgca gctcgccgcg cgttaccgga gtcgacatgt cggtggagtc
     1321 catcatcact acacctcctc gacgtcgagt acaaggcaac acgcttgccg tgccgtcgac
     1381 acggtgtcgg gcgccgggag tgaggcggat gatggtcgag cgttgcgttc cggcaatcgc
     1441 ggatgctttg accgcaaggg ggacagggaa ggtggttcga cgctggatgt gagaagtgac
     1501 atgcgtggga ggcacagcaa gatgcttgcc tgcgcgcgcc cgtgggttta cgcagggttt
     1561 tgaagatgaa gcgtccggcc accggacagc cgtccgaaat ccgtccggac accggacacc
     1621 gcgatcgcct cgacatgggt cgaggccgcc ggcgggtggt cacttcgagg gcctcgaggc
     1681 ggtcggtcaa acgttcggcc agcccctgat cgacgtccgc ctgccggcac agacggccgc
     1741 tcccatggat cggcgctctc gttgctttgc gcttgccggc cgcgctatcg ctaggcgtgt
     1801 cccgacactc gccatgctcg tccacgcccc gctggccggc gatcgcactc gccgcgtgcg
     1861 cgctcgccgg ctgcagccac tcggtgctga tccgcggtga tgacacggcc taccgccgcg
     1921 cgatcgagca ctggccggtc tgatcggtct ggcaaacgtg gtgaattctg ggggcgcggc
     1981 gtccgcgcgg ccgatcgatc gactatggtt ggcaggttgt tcatgatccc ggaagctctg
     2041 tgatcgtcct gtgtggcgcc cagcacgccg agccgacggt gccgcgcgcc ctgcgcgagc
     2101 tcggggtcaa gggcccggtc gcgatggtga cggcgggctg gcaggagcgc gagg
//
LOCUS       DASVOR010002387         2702 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_464307,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002387 DASVOR010000000
VERSION     DASVOR010002387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2702
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464307"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2702
                     /locus_tag="VF516_43950"
     CDS             <1..>2702
                     /locus_tag="VF516_43950"
                     /inference="COORDINATES: protein motif:HMM:NF019323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HEX8114786.1"
                     /translation="IARDPAVAAVVDGARRGEQDGRWLAFRAAEPLPPNAAVEVEIGP
                     GTPSAEGPGVTRKPQSFQFSTYPPLWVVRSQCGWGGPCRPGMPLLIELNNPLDAERFD
                     DRQITITPAIPGVKVFASGQVVSVAGLTTPHTRYSVVVSRGVVDQFGQALGKDETLTW
                     DVGDAAPEFFGPDGLVVLDPSATRPTLDFFSSNYERLSVQLYAVAPADFEAYRTALRN
                     LWNHDHPPQLPGRKVFDQRIATGGGPNQLAETRIDLRPALGPGGLGHAIAVVEPSPWT
                     AKSPPPRLISWVQSTRLGIDVHVDSDRLVAHTSQLDTGKAAAGVALQLAPYGIAGTTD
                     DRGLATLALADGQRRGAHYLVARKGDDVAFVTEDSGIWSESGDWFRQARGKQLAWYVT
                     DDRRLYKPGEEVALKGWLRTIDQARNGDVGGLAGAVTGVSYKVMDAQGNQIAAGTAGV
                     DPVGGFDARFTLPKTRNLGQAEVVFEAQGSLQGNFAHAIEIEEFRTPEFEVSAQASQG
                     PLLVGGGGDATVRAKYFAGGPLAGAPVTWTVSASQTNFTPPNRDDYVFGIWQPWWGPM
                     QLDEAGPIRNTPPKTWTLGATTDATGAHTLHMDFVSVRPAVPMSVTATAQVTDVNRQV
                     IAASAAMIVHPSSLYVGVKTRRPFVERGTPFDVELIGVDLDGKAAPGAKIDVAVVRRD
                     WAFDHGKLKIRDVDPQSCAVVAGKGPAPCSFQTREGGEYQLTATIVDAQGRPNQTRLA
                     FWVSGGERPRARDVAQERVQLIPDKKDYTPGNTAEILVQAPFYPAEGVVSWRRSGIVK
                     AEKITLDGPTAVITVPISDAMVPNLIVQVDLVGLAVRTDGAGVPDPKLPRRPAYAVGS
                     IDLAIPPRQRTLSVDVAPAAARLSPGEKTSIAVAIRDAAGKPVAG"
BASE COUNT          383 a          976 c         1000 g          343 t
ORIGIN      
        1 atcgcccggg acccggcagt cgcggcggtg gtcgacggcg cgcggcgcgg tgagcaggac
       61 ggccgctggc tcgcgttccg cgccgccgag ccgctgccgc cgaacgccgc ggtcgaggtc
      121 gagatcggtc cgggcacgcc atcggccgag ggcccggggg tgacccggaa gccgcagtcg
      181 ttccagttct cgacgtaccc gccgctgtgg gtcgtgcgct cgcagtgcgg ctggggcggc
      241 ccgtgccgcc cgggcatgcc gctgctgatc gagctcaaca acccgctcga cgccgagcgg
      301 ttcgacgacc ggcagatcac gatcacgccg gcgatcccgg gcgtcaaggt gttcgcgtcc
      361 ggccaggtgg tgtcggtggc cggtctgacc acgccgcaca cccggtacag cgtcgtggtg
      421 tcgcgcggcg tggtcgacca gttcggccag gcgctcggca aggacgagac gctgacgtgg
      481 gacgtcggcg acgccgcgcc ggagttcttc gggcccgacg ggctggtcgt gctcgatccg
      541 agcgcgaccc ggccgacgct cgacttcttc agcagcaact acgagcgact gtcggtccag
      601 ctctacgcgg ttgcgccggc cgacttcgag gcctaccgca ccgcgctgcg caacctgtgg
      661 aaccacgacc acccgccgca gctgccgggc cgcaaggtgt tcgaccagcg gatcgcgacc
      721 ggcggcggcc cgaaccagct cgccgagacc cgcatcgacc tccggccggc gctcgggccc
      781 ggcgggctcg gccacgcgat cgcggtggtc gagccgtcgc cgtggaccgc gaagagcccg
      841 ccgccccggc tgatctcgtg ggtccagtcg acccggctgg ggatcgacgt ccatgtcgac
      901 agcgatcgac tggtcgccca caccagccag ctcgacaccg gcaaggccgc cgccggggtc
      961 gcgctgcagc ttgcgcccta cgggatcgcc ggcaccaccg acgaccgcgg cctcgcgacg
     1021 ctcgcgctcg ccgacggcca gcgccggggc gcgcactacc tggtcgcgcg caagggcgac
     1081 gacgtcgcgt tcgtcaccga ggacagcggc atctggagcg aatcgggcga ctggttccgc
     1141 caggcgcgcg gcaagcagct cgcgtggtac gtcaccgacg accgcaggct gtacaagccc
     1201 ggcgaggagg tcgcgctcaa gggctggctg cgcacgatcg accaggcgcg gaacggcgat
     1261 gtcggcgggc tcgccggcgc ggtgaccggc gtgtcgtaca aggtgatgga cgcgcagggc
     1321 aaccagatcg cggcgggcac cgccggggtc gacccggtcg gcgggttcga tgcccggttc
     1381 accctgccga agacccggaa cctcgggcag gccgaggtcg tgttcgaggc ccagggcagc
     1441 ctgcagggca acttcgcgca tgcgatcgag atcgaggagt tccgcacgcc cgagttcgag
     1501 gtctcggcgc aggcgagcca ggggccgctg ctggtcggcg gcggcggcga tgcgacggtc
     1561 cgcgcgaagt acttcgccgg cggcccgctc gccggtgcgc cggtcacctg gacggtcagc
     1621 gcgtcacaga ccaacttcac gccgcccaat cgcgacgact acgtgttcgg gatctggcag
     1681 ccgtggtggg ggccgatgca gctcgacgag gccggcccga tccgcaacac cccgccgaag
     1741 acctggacgc tgggcgccac gactgacgcg accggcgcgc acacgctgca catggacttc
     1801 gtgtcggtcc gcccggcggt gccgatgtcg gtgaccgcca ccgcgcaggt caccgacgtc
     1861 aaccgccagg tgatcgcggc gtcggcggcg atgatcgtgc acccgtcctc gctctacgtc
     1921 ggggtcaaga cccggcggcc gttcgtcgag cggggcacgc cgttcgacgt cgagctgatc
     1981 ggcgtcgatc tcgacggcaa ggccgcgccc ggcgcgaaga tcgatgtcgc ggtggtccgg
     2041 cgcgactggg cgttcgacca cggcaagctc aagatccggg acgtcgatcc gcagtcgtgc
     2101 gcggtggtcg cgggcaaggg gccggcgccg tgctcgttcc agacccgcga gggcggcgag
     2161 taccagctga ccgcgacgat cgtcgacgcg cagggccggc cgaaccagac ccggctggcg
     2221 ttctgggtca gcggcggcga gcggccgcgg gcgcgcgacg tcgcccagga gcgcgtccag
     2281 ctgatccccg acaagaagga ctacacgccg ggcaacaccg ccgagatcct ggtccaggcg
     2341 ccgttctatc ccgccgaggg cgtggtcagc tggcggcgct cgggcatcgt caaggccgag
     2401 aagatcacgc tcgacgggcc gaccgcggtg atcaccgtgc cgatcagcga cgcgatggtg
     2461 ccgaacctga tcgtccaggt cgacctcgtc gggctggcgg tgcggaccga cggcgccggg
     2521 gtccccgatc cgaagctgcc caggcgcccg gcgtacgcgg tcggctcgat cgacctggcg
     2581 atcccgccgc gccagcgcac gctgtcggtc gacgtcgctc cggccgccgc ccggctgtca
     2641 cccggcgaga agacgtcgat cgcggtcgcg atccgcgacg ccgccggcaa gccggtcgcc
     2701 gg
//
LOCUS       DASVOR010002388         3432 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_260574,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002388 DASVOR010000000
VERSION     DASVOR010002388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260574"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="VF516_43955"
     CDS             complement(<1..646)
                     /locus_tag="VF516_43955"
                     /inference="COORDINATES: protein motif:HMM:NF039495.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114787.1"
                     /translation="MTEIGRIRLGQFQTPEGRKGRPVKLDRFRFTSDNEQLIRAVANR
                     YGGEAGQYTPQRSNRTEWEVVSATNDIEVFIPPQNIEPWNEAWRPGTCVRRCDGEREF
                     REAQTKDLEWQACPCNAGRVPAADLCKPTIRIQLMLPEVPPAIGTWRLESHGTYAVSE
                     LAMVAPIVAQAPTVIPASLRLREETRRAWNAEKSKYDTLTFYVPYLVLWSATPQQ"
     gene            complement(733..1962)
                     /locus_tag="VF516_43960"
     CDS             complement(733..1962)
                     /locus_tag="VF516_43960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114788.1"
                     /translation="MTAPAAAPAALPVHVTAEDGSRFYLRTNPDTGVEEHYWSVTTAL
                     SVKNKDGLKWWSAKLAARRAMANLPMLVRSQLVEACGRTWARTEPLRCEKCAECVQRW
                     VELFHVGESERRKHEGSAIHDAVESWIKTGEWRTPVDLAQPYLERHPDIVKMLPPYLE
                     RCRQWVEEYGLRPEDFWASEMTVYHHGHKYAGTCDGALWLSPRTKKAAQLMARILGRL
                     PLPDERILVIIDCKTREGEDTEIYDDHPLQLTAYRNAQTCRPSKVHDREFPMPATHGA
                     VIFQPRPDGYAFRPVRTGPGEFKAFLAFLDGWRWTVEDGATSTQVGTFPMPAGFEWPL
                     PQPEPEPAADAPLPTKKATAKRTRKKAAAPAAQVTPEGHTISGATPPRMAGATLDSLH
                     GGRPPHPDSPYGDNIPF"
     gene            complement(1959..2216)
                     /locus_tag="VF516_43965"
     CDS             complement(1959..2216)
                     /locus_tag="VF516_43965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114789.1"
                     /translation="MIIGEEPTAWIVARRTKDNYHSYDDLALELNTILVSSGAESIKV
                     TSETVRRWHGVAEGNPPATRERPASTATDVPPVTFSNGDDW"
     gene            2394..2651
                     /locus_tag="VF516_43970"
     CDS             2394..2651
                     /locus_tag="VF516_43970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114790.1"
                     /translation="MGRHFPAAPAADTLKELFECLAIVERAAYRDPDPAAGPAFERIL
                     YLVKRASFGNDDHRVMLAQLVARYESVHGVGRVAPDPEVDK"
     gene            2648..3046
                     /locus_tag="VF516_43975"
     CDS             2648..3046
                     /locus_tag="VF516_43975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114791.1"
                     /translation="MTGVDYRDHPVVRCRCCERYGKNYARGLVRDCYVRAAEAGTLER
                     DFPRRVWRGPRLSTESILDEWASLARDPSPGTTTGDIARRIGLSVEALQQTLIRARRR
                     GDERAIYMRHGRSGWRYGDPTAPRRRRNGG"
     gene            3048..>3432
                     /locus_tag="VF516_43980"
     CDS             3048..>3432
                     /locus_tag="VF516_43980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114792.1"
                     /translation="MQHLISNALLVAGIALAASAALVAALYGRDQRRERREHDAEVRA
                     LARQRVAAAALDDRTIEIPILRSPEDTAAHRAHRWVTAGRAWAEQRDREQAKTWRTGY
                     DALIAEQEARWRALGSRWEAELGPID"
BASE COUNT          497 a         1181 c         1203 g          551 t
ORIGIN      
        1 actgttgcgg agtcgccgac cacagcacca gatacggcac gtaaaacgtc agcgtgtcgt
       61 atttcgattt ctcggcattc cacgcgcgcc gcgtctcctc gcgcagccgc agcgacgcgg
      121 ggatcaccgt gggcgcctgc gcgacgatcg gggcgaccat cgccagctca ctgaccgcgt
      181 atgtgccatg cgattcgagg cgccacgtgc cgatcgccgg gggcacctcg ggaagcatga
      241 gctgaatgcg gatcgtcggc ttgcacaggt cggcggccgg cacccggccg gcgttgcacg
      301 ggcaggcctg ccactccaga tctttcgtct gcgcctcgcg gaactcgcgc tcgccgtcgc
      361 agcggcgcac gcacgtgccg ggccgccacg cctcattcca cggctcgatg ttctgaggtg
      421 ggatgaagac ttcgatgtcg ttggtggccg acaccacctc ccactccgta cggttcgagc
      481 gctgcggggt gtactggccg gcctcgccgc cgtaccggtt cgccaccgcc cggatgagct
      541 gctcattgtc ggaggtgaac cggaaccggt ccagcttgac cgggcggccc ttgcgacctt
      601 ccggggtctg gaactgcccc aggcggatcc ggccgatctc ggtcatggcc cgctgaaagt
      661 ctcggggggt gccgtcggat ccgagcccgg gaagttgctt gatcgacacg tcacgtgccc
      721 tttcggggga ggtcagaacg ggatgttgtc gccgtacggc gagtcgggat gtgggggccg
      781 gccaccgtgc aggctgtcga gcgtagcgcc ggccatgcgg ggcggggtgg cccccgagat
      841 ggtgtgtccc tcgggcgtga cctgggcggc cggtgcggcg gccttcttgc gcgtccgctt
      901 cgccgtcgcc ttcttggtcg gcagcggcgc gtcggcggcc ggctccggtt cgggttgcgg
      961 aagcggccat tcgaagccgg ccggcatcgg gaacgtgccc acctgtgttg aggtggcgcc
     1021 gtcctcgacc gtccaccgcc agccgtcgag gaaggcgaga aacgccttga actcgccggg
     1081 gccggtgcgc accggacgga acgcgtaccc gtccggccgt ggctggaaga tcaccgcgcc
     1141 gtgcgtggcc ggcatcggaa actctcgatc gtgcactttg gacggtcgac aggtctgggc
     1201 gttacggtac gcggtgagct gcaacggatg atcgtcgtag atctccgtgt cttcgccctc
     1261 gcgggtcttg cagtcgatga tgaccaggat ccgctcgtcg ggcagcggta gccggcccag
     1321 aatccgggcc atgagctgcg cggccttctt ggtgcgcggc gagagccaca gcgcaccgtc
     1381 gcaggtgccc gcgtacttgt gcccgtggtg gtagaccgtc atctcggagg cccagaagtc
     1441 ctccgggcgc agtccgtact cttcgaccca ctggcggcag cgctccaggt acggcggcag
     1501 catcttgaca atgtccgggt gccgttcgag gtacggctgc gcgagatcga cgggcgtgcg
     1561 ccattcaccg gtcttgatcc acgactcgac ggcatcgtgg atggccgaac cctcgtgttt
     1621 gcgccgttcc gactcgccga cgtggaacag ctcaacccag cgttgcacgc actcggcgca
     1681 cttctcgcag cgcagcggtt ccgtgcgcgc ccaggtgcgc ccgcacgcct cgacgagctg
     1741 ggagcgcacc agcatgggga ggttcgccat ggcccggcga gcggcgagct tcgccgacca
     1801 ccacttgagc ccgtccttgt tcttgacgga cagcgcggtg gtcacgctcc agtagtgctc
     1861 ctcgacgccc gtgtccgggt tggtgcgcag gtagaaacgg gatccgtcct cggcggtgac
     1921 gtgaaccggc agcgcggccg gggcggcggc cggcgctgtc accagtcgtc gccgttggag
     1981 aatgtcaccg gcggcacgtc ggtggcggtc gaggcgggcc gctcgcgggt ggccggcggg
     2041 ttgccctcgg ccacgccgtg ccagcgacga acggtctccg aggtgacctt gatcgactcg
     2101 gcgccggacg agaccaggat ggtgttcagt tccaacgcga gatcgtcata cgagtggtag
     2161 ttgtccttgg tgcgccgtgc gacgatccag gcggtcggct cctcgccgat gatcatcttg
     2221 acggcctggt atttgtcccc cgtaccaggc atacgacctg gcggggcgtg tccttcagcc
     2281 atgatgttca ggggaccacc tcccgaatcc ggttgtcaag ccgcgtgcca gtttaccatg
     2341 gtcgaaatgg cacatgactt gacacgtatg gggtggcgag ggacgctgga cgcatgggcc
     2401 gacactttcc cgccgccccg gcggcagaca cgctcaaaga gctgttcgag tgcctcgcca
     2461 tcgtcgagcg ggccgcctac cgcgaccccg acccggcggc cgggccggcg ttcgagcgca
     2521 tcctgtacct ggtcaagcgg gccagcttcg gcaacgacga ccaccgcgtc atgctcgccc
     2581 agctcgtcgc ccggtacgag tccgtccacg gggtcggacg ggtcgccccg gaccccgagg
     2641 tcgacaagtg accggcgtcg actaccgcga ccatccagtg gtccgctgtc gctgctgcga
     2701 gcggtacggc aagaactacg cccggggcct ggtacgcgac tgctacgtcc gcgcggccga
     2761 ggccggcacc ctggagcgtg acttcccgcg ccgcgtctgg cgaggccccc ggctgagtac
     2821 cgaatccatc ctcgacgagt gggcgagcct cgcccgtgac ccgagtccgg gcaccaccac
     2881 cggcgacatc gcccggcgga tcggcctgtc ggtcgaagcc ctgcaacaga ccctgatccg
     2941 cgcccgacgg cggggcgacg agcgggccat ctacatgcgc cacgggcgca gtggttggcg
     3001 ctacggtgac cctacggcac cccgccgccg tcgaaacgga ggatgacatg caacacctca
     3061 tcagcaatgc gctgctggtg gccggcatcg ccctggcggc ctcggccgcg ctcgtcgccg
     3121 ccctgtacgg ccgtgaccag cgacgcgagc ggcgtgaaca cgatgcggag gtaagggctc
     3181 tcgcccggca gcgggtggcc gccgccgcgc tcgacgaccg caccatcgag atcccgatcc
     3241 tgcgctcgcc ggaagacacc gccgcccacc gcgcccaccg gtgggtcacc gccggccggg
     3301 cctgggccga gcagcgtgac cgcgagcagg ccaagacgtg gcgcaccggg tacgacgcgc
     3361 tcatcgccga gcaggaggcc cgctggcggg cgctgggctc gcgctgggaa gccgaactgg
     3421 ggccgatcga cg
//
LOCUS       DASVOR010002389         5362 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_201614,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002389 DASVOR010000000
VERSION     DASVOR010002389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5362)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5362)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5362
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201614"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="VF516_43985"
     CDS             <1..954
                     /locus_tag="VF516_43985"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8114793.1"
                     /translation="IQLVRKYGGSPTGAHRPGPPPPGMALIDVGTIEVGRNADEIERE
                     CREIGAGCDRELMQREIPRRKVTVPAFFLDRDEVTNAQFAKLLNDYIGMVVVTDDEDY
                     HKPRFVHLNRGSGDRDVLYDLNDWHGGSIDYVGTVDHHRFVVRPGRDLLPAAQMSWYG
                     AKLYCESVGKRLPTEDEWEAAARGRDDRRFPWGNALPRCGDVVIANDGKLAMSGSCPP
                     GDVALRAIGASLQDITPDGVRGLAGGVSEWTSSLYVEGVRSVDPGSVPRETPRVFRGA
                     SWATSLMARSSGRSKRPPSIMGANIGLRCASSVEGSRPGGP"
     gene            976..1398
                     /locus_tag="VF516_43990"
     CDS             976..1398
                     /locus_tag="VF516_43990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114794.1"
                     /translation="MTRPVSDKPARHASPKRTRVTTSAGPSARPATGPTKSALRPTLT
                     RVTGDIDLVAPHGFCASVLLKLVSRDGGHRYYSLWAMVGRHGVDTFSIRYKYRGEDLI
                     RCDLPEGMPVSEESLQPHLVSFLAGEHNEPDGTPIIRD"
     gene            1445..2689
                     /locus_tag="VF516_43995"
     CDS             1445..2689
                     /locus_tag="VF516_43995"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HEX8114795.1"
                     /translation="MNAATWNLVIYAIAGDAAEHQAVLGAIDDMRTALTTDQCHIAVQ
                     VMAKSRTTRYWISADDKIRTEVLPEVVDASHQAPLTGFLNLAGKRFPAAATALTLWAH
                     GSGLDHIHDYRKKPGGPGGGGLGGASPEDPNAGLLAIDQAARLRTPYGRALSARGRLA
                     AWLPRHPGYGCRWGPDPNTGRFLTNVTMKKAIAATMAGQVDVLALNACWMATLEVEYE
                     LRHAAAAIVASQVYAKPWPYRAIVEAMSRRPVQSAEQLAQTLVTAISAEIAADQRNDA
                     VSAFRAGAALDELAAAFDAYAELVTHLIDTDWEAVRDAVVTQVQRLDDPYQADLVSLA
                     KLLGKHDRHAHAAGQAVVHQLEAMRIGNAAYHGHPGLHGLSIFCPKSIEVDLADAYAG
                     TEFRSNAWASFLAKFQPRLAAS"
     gene            complement(2695..>5362)
                     /locus_tag="VF516_44000"
     CDS             complement(2695..>5362)
                     /locus_tag="VF516_44000"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEX8114796.1"
                     /translation="AAPEGLVPPEPTLRLPRNFLPTGYAARLDIDPASSGFEGAIQIT
                     GNVGEKSRVIWLNARGLTVHKAVAQRAGQPDVALTATPRNDDFLELRAAEPLDAGAWT
                     LAIDYAGRFDTLNTAGAFKQTVAGASYVYTQFEAVYARRAFPCFDEPDNKVPWQLTLD
                     VPAQLSAVANAPEAAQSPLPGGKKRVEFAPTRPLPSYLVAFGVGPFEFVDAGKTQRGT
                     PVRIVALAHRAADAAFAAKTSAKLIDLLEGYFGAPYPYPKIDMLSIPITVGFGAMENA
                     GLITYTETSILHDAKASKQRQLGWISVASHELSHQWFGDLVTTAYWDDIWLNEGFATW
                     MARKIVARFEPAWRDDQSELNTRDIALDNDSLVNARQIRQPIKKQDDIFTAFDRITYD
                     KGASILNMFESYLGADTFQRGVRDYIAARAWGNATSTDFVAAISKAAGKDVGPAFATF
                     LEQPGTPEITATVSCEGGKASVALAQQRYVPPGAPTPPATKPWIVPVCVAFDRGGGGQ
                     RGEACTMLDQPTGSIDLSASLPARACPRWVMPNVNARGYYRSVYTPAQVGALRDEAWG
                     QLSWTERRALFRDVRDAVGNGKLPLQLALSLAPKLLAGNDRFTVGPAIQLATGLGRWV
                     PVDLQPRYEAWLRETFGPGAAKADLVPRDSDTLDVEVTRGELIEAVAWRGRDARLVAD
                     AVKLADHWRDLPQSVRGPVLRIAVDARPEIFERTLQDVRTETDRARRQEMLRALASVR
                     DPRRHATALGLMLDKKVDPRETLGMLFSGNFGGRGGGGAAGSEGDAILAVSQAFFREH
                     EAEIMSTMPQDGTSGPFARLSALFTATCRADQREAITDYVQKTFAKLPGGKRILAQNL
                     EQMDQCIARRQQLEPEVRAWLTGAKMPRPAKP"
BASE COUNT          750 a         1927 c         1929 g          756 t
ORIGIN      
        1 atccagctcg tgcgcaagta cggcggctcg ccgaccggcg cgcaccgccc cggcccgccg
       61 ccgcccggca tggccctgat cgacgtcggc acgatcgagg tcggccgcaa cgccgacgag
      121 atcgagcgcg agtgccgcga gatcggtgcg ggctgcgatc gggagctgat gcagcgcgag
      181 atcccgcgcc gcaaggtcac ggtgccggcg ttcttcctcg accgcgacga ggtcaccaac
      241 gcgcagttcg ccaagctgct caacgactac atcggcatgg tggtcgtgac cgacgacgag
      301 gactaccaca agccgcgctt cgtgcacctc aaccgcgggt ctggcgatcg cgacgtcctg
      361 tacgatctga acgactggca cggcggtagc atcgactacg tcggcaccgt cgaccaccac
      421 cgcttcgtcg tccgccccgg ccgcgacctg ctgccggcgg cgcagatgag ctggtacggc
      481 gccaagctgt actgcgaatc ggtcggcaag cggctcccca ccgaggacga atgggaggcc
      541 gccgcgcgcg gccgcgacga ccgccggttc ccgtggggca acgcgctgcc gcgctgcggc
      601 gacgtcgtga tcgccaacga cggcaagctc gcgatgtcgg gctcgtgccc cccgggcgat
      661 gtcgcgctgc gcgcgatcgg cgcctcgctc caggacatca ccccggacgg cgtccgcggc
      721 ctcgccggag gcgtctcgga gtggacgtcg tcgctgtacg tcgagggggt ccgctcggtg
      781 gaccccggca gcgtgccccg cgagaccccg cgcgtgttcc gcggtgcgtc gtgggcgacc
      841 tcgctgatgg cccgctccag cgggcgaagc aagcggccac catcgatcat gggtgccaac
      901 atcggtctcc gttgcgcctc gagtgtcgag gggtcgcgac cggggggacc atagaaaggg
      961 acgatcatgc agaagatgac acgaccggtc tcggacaagc ccgcgcgcca cgcctccccg
     1021 aagcgcaccc gcgtcaccac atccgctggg ccatccgctc ggccggccac agggccgacc
     1081 aagtcggcgc tccggccgac gctcacccgg gtcacgggcg acatcgatct cgtcgcgccc
     1141 cacggctttt gcgcgagcgt gctgctcaag ctggtgagcc gcgatggtgg ccatcgctat
     1201 tattccttgt gggcgatggt cggacggcac ggcgtggaca cgttctcgat ccggtacaag
     1261 taccgcggcg aggacctgat ccggtgcgac ctgcccgagg gcatgccggt gtccgaggag
     1321 tcgctgcagc ctcacctggt cagcttcctg gccggcgagc acaacgagcc cgacggcacc
     1381 ccgatcatcc gggactgagc aaacgcggca tgggcagggc ggaagcgagc ggagcggacg
     1441 tgcaatgaac gcggcgacct ggaacctcgt gatctacgcg atcgccggtg acgcggcgga
     1501 gcaccaggcg gtgctcggag cgatcgacga catgcgcacg gcgctgacca ccgatcagtg
     1561 ccacatcgcc gtgcaggtga tggccaagag ccggacgacc cggtactgga tctcggcgga
     1621 cgacaagatc cgcaccgagg tcctccccga ggtcgtcgac gccagccacc aggcgccctt
     1681 gaccgggttc ctcaacctcg ccggcaagcg gttcccggcg gcggccaccg cgctcaccct
     1741 gtgggcgcac ggcagcggcc tggaccacat ccacgactat cgcaagaagc cgggcggtcc
     1801 cggcggcggc ggcctcggcg gcgcgtcccc cgaggacccg aacgccggcc tgctcgccat
     1861 cgaccaggcc gcccgcctgc gcacgcccta cggccgcgcg ctctcggccc ggggccggct
     1921 cgccgcctgg ctgccgcgcc accccggcta cggctgccgc tggggcccgg atcccaacac
     1981 cggccggttc ctcaccaacg tcacgatgaa gaaggcgatc gccgcgacca tggccggcca
     2041 ggtcgacgtc ctcgccctca acgcgtgctg gatggccacg ctcgaggtcg agtacgagct
     2101 gcgccacgcc gccgcggcga tcgtcgcgtc ccaggtctac gccaagccgt ggccgtaccg
     2161 cgcgatcgtc gaggccatgt cgcgccgccc ggtccagtcc gccgagcagc tggcacagac
     2221 cctcgtcacc gcgatcagcg ccgagatcgc cgccgaccag cgcaacgacg ccgtctctgc
     2281 gttccgcgcc ggcgcggccc tcgacgagct cgccgccgcg ttcgacgcct acgccgagct
     2341 ggtcacccac ctgatcgaca ccgactggga ggcggtgcgc gacgccgtgg tgacccaggt
     2401 ccagcggctc gacgacccct accaggccga cctggtgtcg ctcgccaagc tgctcggcaa
     2461 gcacgatcgc cacgcccacg ccgccggcca ggccgtcgtc caccagctcg aggcgatgcg
     2521 gatcggcaac gccgcctacc acggccaccc cggcctgcac ggcctgtcga tcttctgccc
     2581 caagagcatc gaggtcgacc tcgccgacgc ctacgccggt accgagttcc ggtccaacgc
     2641 ctgggccagc ttcctcgcca agttccagcc ccgcctcgcc gcgtcgtagc cccgttacgg
     2701 cttcgccggc ctgggcatct tggcgccggt cagccaggcg cggacctcgg gctcgagctg
     2761 ctggcggcgc gcgatgcact gatccatctg ctcgaggttc tgcgcgagga tccgcttgcc
     2821 gccggggagc ttggcgaacg tcttctggac gtagtcggtg atcgcctcgc gctggtcggc
     2881 gcggcaggtc gcggtgaaca gcgcgctcag ccgggcgaac gggccggagg tgccgtcctg
     2941 cggcatggtg ctcatgatct cggcctcgtg ctcgcggaag aacgcctgcg acacggcgag
     3001 gatggcgtcg ccctcgctgc cggccgcgcc gccgccgccg cggccgccga agttgccgga
     3061 gaacagcatc cccagggtct cgcgcgggtc gaccttcttg tcgagcatca ggccgagggc
     3121 ggtggcgtgg cgcctggggt cgcgcacgct ggcgagcgcg cgcagcatct cctggcgcct
     3181 ggcgcgatcg gtctcggtcc ggacgtcctg gagggtgcgc tcgaagatct cgggccgggc
     3241 atcgacggcg atccgcagca ccgggccgcg caccgactgc ggcaggtctc gccagtggtc
     3301 ggcgagcttg accgcgtcgg cgaccagcct ggcgtcgcgg ccgcgccagg cgaccgcctc
     3361 gatcagctcg ccgcgggtga cctcgacgtc gagcgtgtcg ctatccctgg ggacgaggtc
     3421 ggccttggcg gcgccggggc cgaaggtctc gcgcagccag gcctcgtagc gcggctgcag
     3481 atcgacgggc acccagcggc cgaggccggt cgcgagctgg atcgccgggc cgacggtgaa
     3541 gcggtcgttg ccggcgagca gcttgggggc gagcgacagc gcgagctgga gcggcagctt
     3601 gccgttgccg accgcgtcgc ggacgtcgcg gaacagtgcg cggcgctcgg tccacgacag
     3661 ctggccccag gcctcgtcgc gcagcgcgcc gacctgggcc ggcgtgtaga cgctgcggta
     3721 gtagccgcgg gcgttgacgt tgggcatcac ccagcgcggg caggccctgg cggggagcga
     3781 cgccgagaga tcgatcgacc cggtcggctg atcgagcatc gtgcaggcct cgccgcgctg
     3841 gccgccgccg cccctgtcga acgcgacgca caccgggacg atccacggct tggtcgccgg
     3901 cggggtcggg gcgccgggcg ggacgtagcg ctgctgggcg agcgcgacgc tggccttgcc
     3961 gccctcgcag gacacggtcg ccgtgatctc cggcgtgccg ggttgctcga ggaaggtcgc
     4021 gaacgccggg ccgacgtcct tgccggcggc cttgctgatc gcggcgacga agtcggtcga
     4081 cgtcgcgttg ccccacgcgc gcgcggcgat gtagtcgcgc accccgcgct ggaacgtgtc
     4141 ggcgccgagg tagctctcga acatgttgag gatgctggcg cccttgtcgt aggtgatgcg
     4201 gtcgaacgcg gtgaagatgt cgtcctgctt cttgatcggc tggcggatct ggcgcgcgtt
     4261 gaccaggctg tcgttgtcga gcgcgatgtc gcgggtgttg agctcggact ggtcgtcgcg
     4321 ccacgccggc tcgaaccggg cgacgatctt gcgcgccatc caggtcgcga acccctcgtt
     4381 gagccagatg tcgtcccagt acgccgtcgt gaccagatcg ccgaaccact ggtgggacag
     4441 ctcgtgcgac gcgaccgaga tccagccgag ctggcgctgc ttggatgcct tggcatcgtg
     4501 caggatcgag gtctcggtgt aggtgatcag gcccgcgttc tccatcgcgc cgaacccgac
     4561 ggtgatcggg atcgagagca tgtcgatctt ggggtacggg tacggcgcgc cgaagtagcc
     4621 ctcgagcagg tcgatcagct tcgcgctggt cttcgcggcg aacgcggcgt cggcggcgcg
     4681 gtgggcgagc gcgacgatgc ggaccggcgt gccgcgctgg gtcttgccgg cgtcgacgaa
     4741 ctcgaacggg ccgacgccga acgcgacgag gtacgacggc agcggcctgg tcggcgcgaa
     4801 ctcgacgcgc ttcttgccgc cgggcagcgg cgactgcgcc gcctcgggcg cgttggccac
     4861 cgcggacagc tgcgccggca cgtcgagcgt gagctgccac ggcaccttgt tgtcgggctc
     4921 gtcgaagcac gggaacgcgc ggcgggcgta caccgcctcg aactgggtgt agacgtacga
     4981 cgcgccggcg accgtctgct tgaacgcgcc ggcggtgttg agcgtgtcga accggccggc
     5041 gtagtcgatc gccagcgtcc acgcgccggc gtcgagcggc tcggcggcgc gcagctcgag
     5101 gaagtcgtcg ttcctgggcg tcgcggtcag cgcgacgtcg ggctggccgg cgcgctgcgc
     5161 gaccgccttg tgcaccgtga ggccgcgcgc gttgagccag atcacccgcg acttctcgcc
     5221 gacgttgccg gtgatctgga tcgcgccctc gaaccccgag ctcgccggat cgatgtcgag
     5281 ccgcgcggcg tagccggtcg gcaggaagtt gcgcggcagg cgcagcgtcg gctccggcgg
     5341 cacgaggccc tccggcgcgg cc
//
LOCUS       DASVOR010002390         4929 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_142960,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002390 DASVOR010000000
VERSION     DASVOR010002390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142960"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VF516_44005"
     CDS             complement(<1..239)
                     /locus_tag="VF516_44005"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEX8114797.1"
                     /translation="MPLLSLADHATFDATDDSGIILDTRAGMYFNLNVTATIMLEAAL
                     ACETIDQVVARLVERIDGSADTLRAGVERLVAELRA"
     gene            complement(217..1764)
                     /locus_tag="VF516_44010"
     CDS             complement(217..1764)
                     /locus_tag="VF516_44010"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase C-terminal domain-containing
                     protein"
                     /protein_id="HEX8114798.1"
                     /translation="MLSGVLAEPATAELAARIRAALVNGRYQDLGQIAGELAGCLVTS
                     KKIYLFKTIMGNSSTIFFRRSGQAVRWSTSPADLIEDPDTDIDRAAIARCCRGEGPFI
                     YPSIDRVRPGQVVILEQHRTTILEYDRITPIALPRRTTLPEYAEVAWKLLRDAVRPYA
                     GSGRVGVLLSGGIDSSSVLTALVDVGADVIAYHLDTEDQLADESAYARAVCEHLDVPL
                     VSIPTDNGAGYLSRSWDFPHPYSHTGYRHLEQAAERVAGDGVTFLATGREGDILFGPA
                     YYHYGMRDVLFGDIRWHEKKEMLCGVLSSRWQLSSILKSCFSNSSLVEGSMPIGENAR
                     RSDFLAPMPGTSDEKVDYDFMAQESTMDLTLFRSRGVLLCSPMGSKDLRRLSMRLPNA
                     YRWIPFQGRLIDKPVLRMMLARRLPDLIWRRGARSWLNSPPQRYALRHSQPLRELIGS
                     PGSHLVRTGIVNPARLARALGQPVSVRRNAEALICSAMVELFLRSMENRFGTAWKGPQ
                     DAPTFAS"
     gene            complement(2130..2300)
                     /locus_tag="VF516_44015"
     CDS             complement(2130..2300)
                     /locus_tag="VF516_44015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114799.1"
                     /translation="MNTKNEQVHQNNDQSVTLDAKVPYEAPALTVMGKVEHLTALLSR
                     GTPDLLSHGNIL"
     gene            2822..4627
                     /locus_tag="VF516_44020"
     CDS             2822..4627
                     /locus_tag="VF516_44020"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8114800.1"
                     /translation="MAPYVDPPEAEGDRPRDRRRLAVLWRLLPLLRPHRARCLLALTT
                     LCGASGIALVYPWAARQAVDAGMSGRTTGRLDAIVALMLGVFAVNATLVWLRHYSISW
                     LGERIIADLRGLVFDRVLTLPLAWFHERRSGELVGRLASDVTVIEGVVGSELSMALRS
                     AIQMIGAMVMLLVIDVSLTLLMLAIVPPITLTTIYFGRRIRRMTRAVQDELAQVSGQV
                     QEAVGAIATVQSFVREPYEARRYRAGIEAAFGQTLRLVRWRSWFFSTAMTAGYIGVAA
                     VIWLGGRALIHGELTAGQLTSFFLYTFIVAGALGDVAGLWGALQRAAGATDRLFAVID
                     TVPEIRDPEPARPLPPGKGWVKLERVSFAYPSRRGHPVLADVDLEVAPGEVVALVGPS
                     GAGKSTILSLLYRFHDVDRGRVLFEGMDARQLALRELRRALAMVAQEPVLFSGTIHDN
                     IGYGRDGATAAEIERAARDAHAHDFIVGFPDGYATVIGERGTKLSGGQKQRIAIARAL
                     LADPRVLILDEATSNLDAESEAAVQAALARLMHGRTTIIVAHRLSTVREADRIVVLDG
                     ARVVEQGRHDELMARRGTYHRLVEHQLIADQRAAS"
     gene            complement(4662..>4929)
                     /locus_tag="VF516_44025"
     CDS             complement(4662..>4929)
                     /locus_tag="VF516_44025"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8114801.1"
                     /translation="TPPATQAAPPPEPRPLPPPDPARRAVGMSIQNAQTALKRLGYDP
                     GPIDNAYGPRTRNAIMQFQRSQHLSATGVLDRDTWSAIVGQLMP"
BASE COUNT          772 a         1620 c         1682 g          855 t
ORIGIN      
        1 gcgcggagct ccgcgaccag gcgctcgacg ccggcgcgca gcgtgtcggc gctgccgtcg
       61 atgcgttcga ccagacgcgc cacgacctga tcgatcgtct cgcacgccag cgcggcctcg
      121 agcatgatcg tcgccgtgac gttgaggttg aagtacatgc cagcgcgggt gtcgaggatg
      181 atccccgagt catccgtggc gtcgaaggtg gcatggtcag ctagcgaaag taggggcatc
      241 ttgggggcct ttccacgcgg taccgaatcg gttttccatg ctgcgcagga acagctcgac
      301 catggccgag cagatcaggg cctcggcatt gcgccggacc gacacgggct ggccaagcgc
      361 cctggccagc cgcgccgggt tcacgatgcc ggtccggacg aggtgggaac ccggactgcc
      421 gatcagctcg cgcaacggtt gcgaatggcg cagggcgtag cgctggggag gcgagttcag
      481 ccatgaacgg gcaccgcggc gccagatgag atccggcagc cgacgggcca gcatcatccg
      541 cagcaccggc ttgtcgatca gcctgccctg aaatggaatc caccggtagg cgttgggcaa
      601 gcgcatcgac agtcgacgca agtccttgct gcccatcggc gagcacagga ggacgccgcg
      661 cgatcggaac agggtcagat ccatggtgga ctcctgggcc atgaagtcgt agtcgacctt
      721 ctcgtcgctc gtgcctggca tgggggcgag aaagtcgctc cggcgcgcgt tctcgccgat
      781 cggcatgctg ccttcgacca gggaagagtt ggagaaacag ctcttgagaa tgctcgacag
      841 ctgccagcgc gaggacagta ccccacacag catctctttc ttctcgtgcc agcgtatgtc
      901 accaaacagg acgtcgcgca taccgtagtg atagtacgcc gggccaaaga gaatatcgcc
      961 ctcgcgcccc gtggccagga atgtcacgcc gtctccggcg acgcgttcgg cggcttgctc
     1021 gaggtggcgg taccctgtgt ggctgtaggg gtgcggaaaa tcccagctcc tggagagata
     1081 gcccgcaccg ttgtcggtcg ggatcgacac gagcggcacg tcgagatgct cgcatacggc
     1141 gcgcgcgtag gccgactcgt cggcgagctg atcctcggtg tccagatggt aggcgatcac
     1201 gtccgccccg acgtccacca gcgcggtgag cacggacgaa gagtcgatgc cgccgctgag
     1261 cagcacaccg acccgcccgc tgcccgcata ggggcgcacg gcatcgcgca ggagcttcca
     1321 ggcaacctcc gcgtactcgg ggagcgtggt gcggcgcggc agcgcgatcg gggtgatccg
     1381 atcgtactcg aggatggtcg tgcggtgctg ctccaggatg accacctgac cgggccggac
     1441 tcgatcgatg gagggataga tgaagggccc ctcgccccga cagcagcgcg cgatcgccgc
     1501 gcggtcgatg tcggtgtccg gatcctcgat caggtctgct ggactggtgg accatcgaac
     1561 cgcttgtccg gaacgccgga agaagatcgt gctgctgttg cccatgatgg tcttgaacag
     1621 gtagatcttc ttcgaggtca ccaggcaccc ggcgagctcg ccggcgatct gcccgagatc
     1681 ctggtagcgt ccattcacca gcgccgcgcg aatccgggcc gccagctcgg cggtcgccgg
     1741 ttcggcgagg acgccagaca gcaatacatg ctcgtgttca gccggagagg gcaggaccgc
     1801 gcccgatccg cgcggattca gccactgcag ggcgtagcac cccttgcaag cgatctcccg
     1861 cccaggatcc gagacgcccc gaagacggat gccattcgtg gcatccgaaa aaacgaactg
     1921 cccggagcag ccggtatagt ctccaatgat ttgattgcca tgtatcatga cgcaccttgg
     1981 atctgacggc tccgggcaat tcatgaacag caagccgcgc ttgcggtctc gacacaccag
     2041 cgtcgaccac gtggccccgc gatcttcact gagtgatgcg ttcgatcgcc tgtcacggct
     2101 cgctgccgtg cctgcgctgc cggcgcaggc tagaggatgt tgccgtggct caacagatca
     2161 ggcgtgcccc tgctgagcag cgccgtgagg tgttcgacct ttcccatgac ggtcagcgcg
     2221 ggcgcctcgt agggcacctt cgcgtccagc gtcacgctct ggtcgttgtt ctggtgaacc
     2281 tgctcgttct tcgtgttcat gactcttcct ttggttgttg tttcaagcga gatctagaga
     2341 ctgcccaggg ctgcggatcc aggaactcgc aactccggat ctgccggctc tgaccatgtc
     2401 atgcttcagg gaagatcgtc aagaatgcac taaccggcaa atacgttact gcaagtacgg
     2461 tgagcccgac agcacagttg aagcttatct gtgttcgttc ttgacacagt cattcacgtg
     2521 cttctaacgc ccgaagcgtg catttacatg tgacctcccg accggatcga tttcgacgat
     2581 cgcaccggcg agcgcggtgc gcccgacggc acaggtccag gcctctccgt gttggctctg
     2641 acacggctgt tcacgcgttt ctaactcacg aaattgcatt tattgtgtga cctcccgacg
     2701 gggagcaatt tcgacaagat ctgcactcgt tccaggtatt tattgtggcg ccacagcaca
     2761 ctctccatgt tcgctcagat cgcgatgaca gcggcgagcc cgcgcgctat gcaaggctgt
     2821 ggtggcaccg tatgtcgatc cgcccgaggc cgagggtgac cgtccgcgcg atcgccggag
     2881 gctcgccgtg ctgtggcgac tgctcccgct gctccggccg caccgcgcgc gctgcttgct
     2941 cgcgctgacc acgctgtgcg gcgcgtcggg tatcgcgctg gtgtatccgt gggcggcgcg
     3001 ccaggcggtc gatgccggaa tgtcgggacg gaccaccggc cggctcgatg cgatcgtggc
     3061 gctgatgctc ggcgtgttcg ccgtcaacgc gaccctggtg tggctgcgcc attacagcat
     3121 cagctggctg ggcgagcgca tcatcgccga cctgcgcggc ctggtgttcg accgcgtgct
     3181 gaccctgccg cttgcgtggt tccacgagcg ccgcagcggc gagctggtcg gccggctcgc
     3241 gtccgacgtc acggtgatcg agggcgtcgt cggcagcgag ctatcgatgg cactgcgcag
     3301 cgcgatccag atgatcggcg cgatggtgat gctgttggtg atcgacgtct cgctcacact
     3361 gctgatgctg gcgatcgtcc cgccgatcac gctcaccacg atctacttcg gccgccggat
     3421 ccgccggatg acgcgcgccg tgcaggacga gctcgcgcag gtctcgggcc aggtccagga
     3481 agcggtcggc gcgatcgcca ccgtgcagtc gttcgtccgc gagccctacg aggctcgccg
     3541 ctaccgcgcc gggatcgagg ccgcgtttgg ccagacgctc cggctggtgc gctggcggtc
     3601 gtggttcttc tcgaccgcga tgaccgcggg ctacatcggt gtcgccgcgg tgatctggct
     3661 cggtggtcgc gcgctgatcc acggcgagct gaccgccggg cagctgacgt cgttcttctt
     3721 gtacacgttc atcgtcgccg gcgcgctcgg cgatgtcgcc gggctgtggg gtgcgctgca
     3781 gcgcgcggcg ggcgccaccg accggctgtt cgcggtgatc gacacggtgc ccgagatccg
     3841 cgatcccgag ccggcgcgcc cgctccctcc cggcaagggc tgggtgaagc tcgagcgcgt
     3901 gtcgtttgcg taccccagcc ggcgcggcca cccggtgctg gccgatgtcg atctcgaggt
     3961 cgcgcccggc gaggtcgtcg cgctggtcgg cccgtcgggc gccgggaagt cgacgatcct
     4021 gtccctgctc tatcgcttcc acgacgtcga ccgcggccgc gtgctgttcg agggcatgga
     4081 cgcccggcag ctcgcgctcc gcgagctgcg ccgcgcgctc gcgatggtcg cgcaggagcc
     4141 ggtgctgttc tccgggacga tccacgacaa catcggctac ggccgggacg gcgcgaccgc
     4201 ggccgagatc gagcgcgccg cgcgcgacgc ccacgcccac gacttcatcg tggggtttcc
     4261 cgacggctac gccaccgtga tcggcgagcg cggcaccaag ctgtcgggcg gccagaagca
     4321 gcggatcgcg atcgcgcgcg cgttgctcgc cgacccgcgc gtgctgattc tcgatgaggc
     4381 gacctcgaac ctcgacgccg agagcgaggc cgcggtacag gcggcgctcg cgcggctgat
     4441 gcacggccgc acgacgatca tcgtcgccca ccggctgtcc accgtgcgcg aggccgatcg
     4501 catcgtcgtg ctcgacggcg cccgcgtggt cgagcagggc cgccacgacg agctgatggc
     4561 gcggcgcggg acgtaccacc gcctggtcga gcaccagctg atcgcagatc agcgcgccgc
     4621 atcgtgacgc gcggcgcgtc cctgccaccg cgaagagggt gctacggcat cagctgtccg
     4681 acgatcgcgc tccaggtatc gcgatcgagg acgccggtgg cggacaggtg ctgcgaccgc
     4741 tgaaactgca tgatggcatt gcgcgtccgg gggccatagg cgttgtcgat cggcccggga
     4801 tcgtagccga gccgcttgag ggcggtctgc gcgttctgga tgctcatgcc gacggctcgc
     4861 cgcgcgggat ccggcggcgg caggggccgc ggttcgggcg gcggcgcggc ctgcgtggcc
     4921 ggcggcgtg
//
LOCUS       DASVOR010002391         2392 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_172297,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002391 DASVOR010000000
VERSION     DASVOR010002391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172297"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1560
                     /gene="acnA"
                     /locus_tag="VF516_44030"
     CDS             <1..1560
                     /gene="acnA"
                     /locus_tag="VF516_44030"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEX8114802.1"
                     /translation="VGSILGDKCGADAAAIDTWSSEGGHAAPGNLGATRQVTTAHGSF
                     ELGHGAVVIAAITSCTNTSNPSVLMAAGLLARAAVARGLRSRPWVKTSLAPGSQVVTD
                     YLTEAGVMADLETLGFHLAGYGCTTCIGNSGPVDDAIAAAVKDGNLVVASVLSGNRNF
                     EGRVNPVVRANYLASPPLVVAYALAGTMNIDLETEPLGTGSDGRPVFLRDVWPSPAAV
                     ADAMRKAIRRDQYQQRYAHVLDGDAEWRALQVPAGDTFAWDGPSTYIRKPSFFDDLGP
                     TPRPLTDLTGARILAILGDSITTDHISPAGNIAKGSPAARYLDSHGVKPADYNQYGAR
                     RGNHEVMVRGTFANIRLKNLMLPGVEGGYTKYFAKAGAAEQLAIYDAAMKYAADGVPL
                     VVLAGELYGSGSSRDWAAKGTLLLGVRAVIAKSFERIHRSNLIGMGVLPLQFLPGEDA
                     ASHGLTGEETITILDIASGVTPKKRLTVHAGGKSFEVIARVDTPQEVEYVTHGGILQY
                     VLRQRAAQTKP"
     gene            1770..2291
                     /locus_tag="VF516_44035"
     CDS             1770..2291
                     /locus_tag="VF516_44035"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8114803.1"
                     /translation="MSHYTIRTLVTDDFAALATLENDVFAAMGEAVLCPHYLRLCTEI
                     FSESCFLALDGERPVGYLLSFVRGREAFCTTLAVIPEHQRTRVTVQLIGAFVRHVVDR
                     VDVCWFTVKEDNAPARALHRMLGAVEVGRRTDYYGPGDERLVAKIDRAIFDQMRGKYE
                     RLGFLPPRTAEAA"
BASE COUNT          351 a          905 c          807 g          329 t
ORIGIN      
        1 gtcggctcga tcctcggcga caagtgcggc gccgacgccg ccgcgatcga cacctggtcg
       61 agcgagggcg gccacgccgc gcccggcaac ctcggcgcga cccggcaggt caccaccgcg
      121 cacggcagct ttgagctcgg ccacggcgcg gtcgtgatcg cggcgatcac cagctgcacc
      181 aacacgtcca acccgtcggt gctgatggcc gccggcctgc tcgcccgggc cgcggtcgcg
      241 cgcggcctca ggtcgcggcc gtgggtcaag acctcgctgg cgcccggctc gcaggtcgtc
      301 accgactacc tcaccgaggc cggcgtgatg gccgacctcg agaccctcgg cttccacctc
      361 gccggctacg gctgcacgac ctgcatcggc aactcgggcc cggtcgacga cgcgatcgcc
      421 gccgcggtca aggacggcaa cctggtcgtc gcctcggtgc tgtccggcaa ccgcaacttc
      481 gagggccggg tcaacccggt cgtgcgcgcc aactacctcg cctcgccgcc gctggtcgtc
      541 gcctacgcgc tcgccggcac gatgaacatc gacctcgaga ccgagccgct ggggaccggc
      601 tcggacggaa ggccggtgtt cctgcgcgac gtctggccgt cgccggccgc ggtcgccgac
      661 gccatgcgca aggcgatccg gcgcgaccag taccagcagc gctacgccca cgtgctcgac
      721 ggcgacgccg agtggcgcgc gctccaggtc ccggccggcg acacgttcgc gtgggacggg
      781 ccctcgacct acatccgcaa gccgagcttc ttcgacgacc tggggccgac gccgcggccg
      841 ctcaccgacc tcaccggcgc gcgcatcctc gcgatcctcg gcgactcgat caccaccgac
      901 cacatctcgc cggccggcaa catcgccaag ggcagcccgg ccgcgcgcta cctcgacagc
      961 cacggcgtca agcccgccga ctacaaccag tacggcgcgc ggcgcggcaa ccacgaggtc
     1021 atggtgcgcg gcacgttcgc caacatccgg ctcaagaacc tgatgctgcc cggcgtcgag
     1081 ggcggctaca cgaagtactt cgcgaaggcc ggcgccgccg agcagctcgc gatctacgac
     1141 gcggcgatga agtacgccgc cgacggcgtc ccgctggtcg tgctcgccgg cgagctctac
     1201 ggcagcggca gctcgcgcga ctgggcggcc aagggcacgc tgctgctcgg cgtgcgcgcc
     1261 gtgatcgcga agagcttcga gcgcatccat cgctccaacc tgatcggcat gggcgtcctg
     1321 ccgctgcagt tcctgcccgg cgaggacgcg gcgagccacg gcctgaccgg cgaggagacg
     1381 atcacgatcc tcgacatcgc gtccggcgtc acccccaaga agcggctcac cgtccacgcc
     1441 ggcggcaagt cgttcgaggt gatcgcccgc gtcgacaccc cgcaggaggt cgagtacgtc
     1501 acccacggcg gaatcctgca gtacgtgctg cgccagcgcg ccgcccagac gaagccttag
     1561 cccggctcgc tgcgacgaaa ccgacgtgat ggtgaggccg gacggtcacg ccggcgtgac
     1621 ctgcgagaac cggcggggtg ggagcgtgtg ctcgccacag gttcgcgatc gttttctagc
     1681 gagcgtgtcg tcgagacgtc gcacgcgctg cggctcggag tcacccatct gcaacgcacg
     1741 tgggtcagcc tgttcgcaca gggagatcca tgagccacta cacgatccgg acgctggtca
     1801 ctgacgactt cgctgccctc gcgacgctcg agaacgacgt gtttgccgcg atgggcgaag
     1861 cggtgctgtg tccgcattac ctgcggctgt gcaccgagat cttctccgaa tcgtgcttcc
     1921 tcgccctcga cggcgagcgg ccggtcggct acctgctgtc gttcgtgcgc ggccgcgaag
     1981 cgttctgtac cacgctcgcg gtgatccccg agcaccagcg cacgcgggtc accgtgcagc
     2041 tgatcggcgc gttcgtccgc cacgtggtcg atcgcgtcga cgtgtgctgg ttcaccgtca
     2101 aggaggacaa cgccccggcg cgcgccctcc accgcatgct cggcgcggtc gaggtcggcc
     2161 ggcgcaccga ctactacggc ccgggcgacg agcgcctggt cgcgaagatc gaccgcgcga
     2221 tcttcgacca gatgcgcggc aagtacgagc gcctcggctt cctgccgccg cgcaccgcgg
     2281 aggccgcgtg atcgcccggc tcgccgggct cgcgatcgac gcgcggttcg cgatccccgc
     2341 cgcgatgccc gggatcctgg agccggccgc gcgcgcccgc gcggcgcgct gg
//
LOCUS       DASVOR010002392         2226 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_260625,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002392 DASVOR010000000
VERSION     DASVOR010002392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2226
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_260625"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="VF516_44040"
     CDS             complement(<1..347)
                     /locus_tag="VF516_44040"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HEX8114804.1"
                     /translation="MQKPVLLAVALAAGCGSHPGQPQSQTAAAQHGIYVEDLDRSADP
                     CTDFFAFSNGTWRKQNPIPASMTRWSRRWKAGEDAKDRLKDILEEVSAKRDWPKASVE
                     QLIGDFYGGCMDQA"
     gene            complement(482..919)
                     /locus_tag="VF516_44045"
     CDS             complement(482..919)
                     /locus_tag="VF516_44045"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEX8114805.1"
                     /translation="MKFMMIVKANQESEAGAMPDEKMLSAMARYNEELTRAGVLLDLS
                     GLAPSSQGARVRFSAGGKRAVIDGPFTEAKELIAGYWLIQVKSREEAIEWAKRIPFEP
                     DPKTGAEPEVELRRLFELDDFAPSPAVEHHRKVGEQLAQSQNK"
     gene            complement(1041..>2226)
                     /locus_tag="VF516_44050"
     CDS             complement(1041..>2226)
                     /locus_tag="VF516_44050"
                     /inference="COORDINATES: protein motif:HMM:NF014722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEX8114806.1"
                     /translation="SRRADGRAVLRSSLRELVCSEAMHHLGVPTTRALSLVTTGEPVV
                     RDLLYDGHPADEPGAVVCRVAPSFLRFGSYEIHAARGDHDTLRRLVRFTLERFYPAYA
                     GGDRLDVAGWFAEVAQRTAWLVSEWLRVGFVHGVMNTDNMSILGLTIDYGPYGWIEPF
                     DPDWTPNITDASGKRYRFGNQPAVARWNLAQLAGALAPLVDDLDPLRRTVDRFAGELA
                     ALHRHSSLRKLGLTGRGDTATDDDDLLATLGALLVEIETDMTVFFRALGQVPVADPEA
                     AIAALGEAYYAPDAITAVQHAAIRGWLGRYAARLAVEGIDPAERRRRMDAINPRYVPR
                     NYLVQQVIEATDRGDRAALPELLDVLRRPYDDQPGRERFARKRPDWARHRPGCSMLSC
                     SS"
BASE COUNT          295 a          798 c          798 g          335 t
ORIGIN      
        1 gcctggtcca tgcagccgcc gtagaagtcg ccgatcagct gctcgacgct ggccttcggc
       61 cagtcgcgct tcgccgacac ctcctcgagg atgtccttga gccggtcctt ggcgtcctcg
      121 cccgccttcc agcgccggct ccaccgcgtc atcgacgccg ggatcgggtt ctgcttgcgc
      181 caggtgccgt tcgagaacgc gaagaagtcg gtgcacggat cggccgagcg atcgaggtcc
      241 tcgacgtaga tgccgtgctg ggcagcggcg gtctgggact ggggctgccc cgggtgcgat
      301 ccacacccgg cggcgagcgc gacggcgagc aagacaggct tctgcatcgc ggccatgatg
      361 ccgagtctgt ccggccgcgc gccagtgccg cgctcgcgtt tgcgatcgca agggacgcgc
      421 cgcgcgcctc gacgggtggt cgcgccggtc gcgccggtcg cagcgacgtc gcagccggcc
      481 gttacttgtt ctggctctgg gcgagctgct cgccgacctt gcggtggtgc tcgaccgccg
      541 ggctgggcgc gaagtcgtcg agctcgaaca gccggcgcag ctcgacctcg ggctcggcgc
      601 cggtcttcgg atcgggctcg aacgggatcc gcttggccca ctcgatcgcc tcctcgcggg
      661 acttgacctg gatcagccag tagcccgcga tcagctcctt ggcctcggtg aacgggccgt
      721 cgatcacggc gcgcttgccg ccggccgaga accggacgcg cgcgccctgc gagctcggcg
      781 ccaggcccga caggtcgagc agcacgccgg cgcgggtcag ctcctcgttg tatctggcca
      841 tcgccgacag catcttctcg tccggcatcg cgcccgcttc cgactcctgg ttggccttca
      901 cgatcatcat gaacttcatg gcgggttctc ctggttggct tggggtctca tggacgcgac
      961 gagcgaaggc ctccgcgatc gacacggcct gcggtttttt ttcaagcggt gcaagtcctc
     1021 gagatcgccg agatccggga tcacgagctg cacgacagca tcgagcagcc cgggcggtgc
     1081 cgcgcccagt cggggcgttt ccgggcgaac cgctcgcggc cgggctggtc gtcgtacggc
     1141 cggcgcagta cgtcgagcag ctcgggcagc gccgcgcgat cgccgcgatc ggtcgcctcg
     1201 atcacctgct ggaccaggta gttgcgcggc acgtagcgcg ggttgatcgc gtccatccgg
     1261 cgccgccgct cggccgggtc gataccctcg acggcgagcc gcgccgcgta gcggccgagc
     1321 cagccgcgga tcgccgcgtg ctgcacggcg gtgatcgcgt cgggcgcgta gtaggcctcg
     1381 ccgagcgccg cgatcgcggc ctcggggtcg gccaccggca cctggcccag cgcgcggaag
     1441 aacacggtca tgtcggtctc gatctcgacc agcagcgcgc cgagcgtggc cagcaggtcg
     1501 tcgtcgtcgg tcgcggtgtc gccgcggccg gtcagcccga gcttgcgcag cgagctgtgg
     1561 cggtgcagcg ccgcgagctc gccggcgaac cggtcgaccg tgcggcgcag cggatcgagg
     1621 tcatcgacca gcggcgccag cgcgccggcg agctgcgcga ggttccagcg cgcgaccgcg
     1681 ggctggttgc cgaaccggta gcgcttgccc gacgcgtcgg tgatgttcgg cgtccagtcc
     1741 ggatcgaacg gctcgatcca gccgtacggg ccgtagtcga tcgtcagccc gaggatcgac
     1801 atgttgtcgg tgttcatcac gccgtgcacg aagccgaccc gcagccactc ggagacgagc
     1861 cacgcggtgc gctgcgcgac ctcggcgaac cagccggcga cgtcgagccg gtcgccgcct
     1921 gcgtaggccg ggtagaaccg ctcgagcgtg aaccgcacca gccggcgcag cgtgtcgtga
     1981 tcgccgcgcg cggcgtggat ctcgtagctg ccgaaccgca ggaacgatgg cgcgacccgg
     2041 cacaccaccg cgccgggctc gtcggccgga tgcccgtcgt acaggaggtc gcgcacgacc
     2101 ggctcgccgg tggtcaccag cgacagcgcg cgcgtcgtcg gcaccccgag gtgatgcatc
     2161 gcctcgctgc acaccagctc gcgcagcgac gagcgcagca ccgcgcgccc gtcggcccgc
     2221 cgcgag
//
LOCUS       DASVOR010002393         3296 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_376957,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002393 DASVOR010000000
VERSION     DASVOR010002393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3296)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3296)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3296
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_376957"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VF516_44055"
     CDS             complement(<1..576)
                     /locus_tag="VF516_44055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114807.1"
                     /translation="MRRCTTLLFLAGCGFSSQASAPDAPIAPIDALADAASASGNDVC
                     VAGVVDVCGMPDPTMAFDVSTAATLNTDSDARCKPVTLAGQAICLIYATEVTIASTGS
                     LTVTGTRPLALVSASTMGIAGAIDAGSHGAQIGPAADDGSCAFATSPENDLGGAGGGA
                     GGSFTLAGGNGGIGDTDNSLGGDGTGLGGMHG"
     gene            616..1317
                     /locus_tag="VF516_44060"
     CDS             616..1317
                     /locus_tag="VF516_44060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HEX8114808.1"
                     /translation="MISATRSTPRFRGFDRTAMQFWHELAAEMSKEWFVANRSRYEAL
                     WRLPMLALIEDIARRLAPVYRPLRLDPPGLLRIHRDLRFSRDRTPYKTQITAVLRPAG
                     APIAHVGNAALYLEIGLDGEYAGVGCYQFDGDRMGRWRRAVTGAAGARLLAILDRLRR
                     GGYTVSDRDDYRRIPKGFAPDHPRAALLKKRGLACAFPDIPAGLLHEPALADWLFRHC
                     RATAPLVIWLQRHVT"
     gene            complement(1364..>3296)
                     /locus_tag="VF516_44065"
     CDS             complement(1364..>3296)
                     /locus_tag="VF516_44065"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HEX8114809.1"
                     /translation="MSTDGSRLVFTPEHPGAAVHWTFGPTDTETLRFPVGAWNVFDVS
                     ADARVVVLPREGDLVVWRLDRTATQEVISRKVGNAQFALSRDGKWLAYVKGAASIELA
                     GLEGQPTRTAQGLAQTVVQMQIDPGSDVLLAALDDGRLLRWRIRDGQSLPTLRARRPV
                     LSISFDWTGDEMALGLEGGGVELWKKPWIAPIELMRSSSGVMNVSFAPDDPWLAVATR
                     DGTVSAWGFSGVSLGEPVRFDGLARAWAQERGSLLGVSLGGTSARWMVPLRHAPRQYD
                     LSGIVGNAWFISEDGFFAIGEHSVLEQRGDRGESRPRYRSALYVSPDRSHSATAYTNG
                     FYLREGMPSQLNSDRADKLLVRAMVDSAMFSADGKRLVAAGGGKGYLFDVATGVALGT
                     PIDGVSQAWLNQDGTILATKGNDVGLTLWQCASDGSLTRLPDVDTRVVSSVAFSRDSR
                     TLVLATENEARVWSLRDRRFVGRTVAHDAAILNVGFSPDGRWIVTTSTDKRARIWEAA
                     SGLPASDWFEHDGSVTAASFSPSGRKLLTASTDGLVRVWDLAGTGDATGQDRRWLARL
                     AEILSGMRIDPETNEAVPAPAAFATLESLRGEIQHACPGTLIDPPGCASATTGLIRSV
                     IGTSGDRPPFGPVQTAAAR"
BASE COUNT          505 a         1228 c         1075 g          488 t
ORIGIN      
        1 gccatgcatg ccgccgaggc cggtgccatc gccgcccagg ctgttgtcgg tgtcgccgat
       61 gccgccgttg ccgccggcca gcgtgaagct gccgcccgct cccccgccgg cgccgcccag
      121 gtcattctcg gggctggtcg cgaacgcgca gctcccgtcg tcggcggcag ggccgatctg
      181 cgcgccatgg ctgccggcgt cgatcgcgcc ggcgatcccc atcgtcgacg ccgacaccag
      241 cgcgagcggc cgcgtcccgg tcaccgtcag cgagccggtc gacgcgatcg tcacctcggt
      301 cgcgtagatc aggcagatcg cctggccggc cagggtcacc ggcttgcagc gcgcgtcgct
      361 gtcggtgttc agcgtggccg cggtcgagac gtcgaacgcc atcgtcggat cgggcatgcc
      421 gcagacgtcc accacgccgg ccacgcacac gtcattgccg ctcgccgagg ctgcgtccgc
      481 cagcgcgtcg atcggcgcga tcggcgcgtc cggggcgctc gcctgggacg agaacccgca
      541 ccccgcgaga aacagcagcg tggtacaacg tcgcatgatt ccagcgatgg taatcacacc
      601 tcacacccgg cagctgtgat ctcggccacc cgcagcacgc cgcggttccg cggcttcgat
      661 cgcaccgcga tgcagttctg gcacgagctg gccgccgaga tgtcgaagga atggttcgtc
      721 gcgaaccgct cgcgctacga ggcgctgtgg cgcctgccga tgctcgcgct gatcgaggac
      781 atcgcgcgcc ggctcgcccc ggtgtaccgc ccgctccggc tcgacccgcc cggcctgctg
      841 cgcatccatc gcgacctgcg gttctcgcgc gaccggacgc cgtacaagac ccagatcacc
      901 gcggtgctgc ggcccgccgg cgcgccgatc gcccacgtcg gcaacgccgc gctctacctc
      961 gagatcgggc tcgacggcga gtacgccggc gtcggctgct accagttcga cggcgaccgg
     1021 atgggccggt ggcgccgcgc ggtcaccggc gcggcgggcg ccaggctgct cgcgatcctc
     1081 gaccggctgc gccgcggcgg ctacacggtg tccgaccgcg acgactaccg ccgcattccg
     1141 aagggcttcg cccccgacca tccgcgcgcc gcgctgctca agaagcgcgg cctggcctgc
     1201 gcgttccccg acatcccggc cggcctgctc cacgagcccg cgctcgccga ctggctgttc
     1261 cgccactgcc gggcgaccgc tccgctcgtg atctggctgc agcgccacgt cacctaggtc
     1321 tccgtcgctg gcgtacggaa ggaggacgct cccagcggcc tggtcagcgt gcagctgccg
     1381 tctgcacggg cccgaaaggc ggccgatcgc cgctcgtgcc gatcacggac cggatgaggc
     1441 cggtcgtggc ggaagcgcat ccgggcggat cgatcagggt ccccgggcac gcatgctgga
     1501 tctcaccgcg cagtgactcg agggtggcga acgccgcggg tgccggaacg gcttcattgg
     1561 tctccgggtc gatgcgcatt cccgacagga tctctgccag ccgggccagc cagcgcctgt
     1621 cctggccggt ggcgtcgccg gtccccgcga gatcccagac ccgcacgagt ccgtcggtgg
     1681 aagcggtgag cagctttcgc cccgaagggg agaacgacgc cgccgtgaca ctgccgtcgt
     1741 gctcgaacca gtcgctcgcc gggagcccgc tggccgcctc ccagatccgc gcacgcttgt
     1801 ccgtcgaggt ggtcacgatc cagcgaccat caggactgaa gccaacgttg aggatcgccg
     1861 catcgtgcgc gacggtgcgt ccgacgaagc gccggtctcg caggctccag acgcgggcct
     1921 cgttctccgt ggccagcacg agggttcgac tgtctcgact gaacgccacg ctggacacga
     1981 cacgcgtgtc gacatctgga agcctggtca aggagccatc gctcgcgcat tgccagagcg
     2041 tcagtcccac gtcgttaccc ttcgtcgcga ggatcgtgcc gtcctggttg agccaggctt
     2101 gcgacacgcc atcgatcggc gtgccgagcg ccacgccggt ggccacatcg aacagatagc
     2161 ccttgccccc gccagccgcg acgagccgct tgccatcggc gctgaacatc gcgctatcca
     2221 ccatcgcgcg cacgagcagc ttgtcggcac ggtccgaatt gagctgggac ggcattccct
     2281 ccctcaggta gaagccgttg gtgtacgccg tggccgaatg gcttcgatcg ggtgagacgt
     2341 acagtgccga ccggtatcga ggccggctct cgccgcggtc gccgcgttgc tccagcacgc
     2401 tgtgctcgcc gatcgcaaag aacccatctt ccgagatgaa ccaggcgttc ccgacgatcc
     2461 cggacaggtc atactggcgc ggcgcgtgtc tgagcgggac catccaccgc gcgctggtac
     2521 cgccgaggga aacgcccagc aggctaccgc gttcctgggc ccaggccctg gccagcccat
     2581 cgaaacgcac cggttcgccg agcgaaacac cggagaagcc ccacgcgctc acggtcccat
     2641 cgcgcgttgc caccgcaagc catgggtcat ccggtgcaaa gctgacattc atcacgccgg
     2701 agctcgaccg catcagctcg atcggcgcaa tccagggctt cttccatagc tccacgccgc
     2761 cgccctccag gcccagcgcc atctcgtcgc ccgtccagtc gaaggagatc gagaggacgg
     2821 gccggcgcgc gcgcagcgtg gggagcgact gaccatcgcg aatgcgccag cgcagaagcc
     2881 tcccatcgtc cagcgcagcg agcagcacgt cggatccagg gtcgatctgc atttgaacga
     2941 cggtctgcgc caggccctga gcagtccgcg tcggctggcc ttccaggccc gcgagctcga
     3001 tcgatgccgc gcccttcaca taggccaacc acttaccatc ccggctcaac gcgaactgtg
     3061 cgttcccgac cttgcgggag atcacctcct gtgtcgccgt gcgatcgagc cgccacacga
     3121 cgagatcgcc ctctctcggg agcaccacga cccgggcatc ggcgctgacg tcgaacacat
     3181 tccaggcacc gacgggaaac cgaagggtct cggtgtcggt cggcccgaac gtccagtgca
     3241 ctgcggcccc cgggtgctcg ggagtgaaca ccaggcgact gccgtcagtg ctcatg
//
LOCUS       DASVOR010002394         1644 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_434689,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002394 DASVOR010000000
VERSION     DASVOR010002394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434689"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VF516_44070"
     CDS             complement(<1..1394)
                     /locus_tag="VF516_44070"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase"
                     /protein_id="HEX8114810.1"
                     /translation="MSDTIDRAIAIVGVGAILPDAPDATRFWANLTAGRYSIGEVPAD
                     RWDPARYYDPDPHAADKTYSKIGGWVREFAWDPARWKLPVPPRVGDAMDAGQKWAVTC
                     TREALIDAGHPDRPFDHARTAVILGNAMAGEKHYMTALRVFFPEFAHALADAPSFAAL
                     DPRLREAIAAESRARLGDAVPPITEDTMPGELSNCIAGRVANLFDFHGPNYVVDAACA
                     SAVAALSTSIAGLVAGDYDAVVTGGIDRNMGASSFVKFCKIGALSATGTRPYADGADG
                     FVMGEGAVVFVLQRLADAERAGRRIYAVVRGVGGSSDGRGKGITAPNPIGQRLAVERA
                     WHDAGLSPATASLIEGHGTSTRVGDVVEVEALGEVLRASGAAPRSIPLGSVKSNIGHL
                     KAAAGAAGLLKAALALHHTVLPPSLGFARPNPNIDFERSPLFVHTALGPWAEPACGVR
                     RAGVSAFGFGGTNF"
     gene            complement(1460..>1644)
                     /locus_tag="VF516_44075"
     CDS             complement(1460..>1644)
                     /locus_tag="VF516_44075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114811.1"
                     /translation="RKVGGDGLAHLVGAGGGGDDGGDALAASLGVPAIQRAQAGQLAD
                     LDRIADALYRRGSFST"
BASE COUNT          219 a          621 c          601 g          203 t
ORIGIN      
        1 tggaagttgg tgccgccgaa cccgaacgcg ctgaccccgg cgcgccgcac gccgcacgcc
       61 ggctcggccc acggccccag cgcggtgtgg acgaacagcg gcgagcgctc gaagtcgatg
      121 ttcgggttgg gccgcgcgaa ccccaggctc ggcggcagca ccgtgtggtg cagcgcgagc
      181 gccgccttga gcagcccggc cgcgcccgcc gcggccttga ggtggccgat gttcgacttc
      241 accgagccga gcgggatgct gcgcggcgcc gcgccggacg cgcgcagcac ctcgccgagc
      301 gcctcgacct cgaccacgtc gccgacccgg gtcgacgtgc cgtgaccctc gatcagcgac
      361 gcggtcgccg gcgacagccc cgcgtcgtgc caggcgcgct cgaccgccag ccgctggccg
      421 atcgggttgg gcgcggtgat ccccttgccc ctgccatcgc tcgagccacc gacgccgcgc
      481 accaccgcgt agatccggcg gccggcgcgc tcggcgtcgg ccagccgctg gagcacgaac
      541 accaccgcgc cctcgcccat cacgaagccg tcggcgccgt cggcgtacgg ccgggtcccg
      601 gtcgccgaca gcgcgccgat cttgcagaac ttgacgaacg agctcgcccc catgttgcga
      661 tcgatgccgc cggtgaccac cgcgtcgtag tcgccggcga ccagccccgc gatcgacgtc
      721 gacagcgcgg cgaccgccga cgcgcacgcc gcgtcgacca cgtagttcgg gccgtggaag
      781 tcgaacaggt tggcgacccg gccggcgatg cagttcgaca gttcgcccgg catggtgtcc
      841 tcggtgatcg gcggcaccgc gtcgcccagc cgcgcgcgcg actcggcggc gatcgcctcg
      901 cgcagccgcg gatcgagcgc cgcgaagctc ggcgcgtccg ccagcgcgtg cgcgaactcg
      961 gggaagaaca cgcgcagcgc ggtcatgtag tgcttctccc cggccatcgc gttgccgagg
     1021 atcaccgcgg tccgcgcgtg atcgaacggc cggtcgggat ggccggcgtc gatcagcgcc
     1081 tcgcgggtgc aggtcaccgc ccacttctgg ccggcgtcca tcgcgtcgcc gacccggggc
     1141 ggcaccggca gcttccagcg cgccggatcc cacgcgaact ctcgcaccca gccgccgatc
     1201 ttcgagtacg tcttgtccgc ggcgtgcgga tcgggatcgt agtagcgcgc gggatcccag
     1261 cggtcggccg ggacctcgcc gatcgagtac cggccggcgg tcaggttggc ccagaagcgg
     1321 gtggcgtcgg gcgcgtccgg aaggatggcg ccgaccccga cgatggcgat cgcgcggtcg
     1381 atggtgtcgg acatggttgc ctcgggttgc gagaaacgcc gcaccaggcg gcgagccagg
     1441 tggtgagcca ggtggtgagc taggtggaga acgagccgcg ccggtacagc gcgtcggcga
     1501 tccgatcgag atcggcgagc tggccggcct gggcgcgctg gatcgcaggc accccgagcg
     1561 aggcggccag cgcgtcgccg ccgtcgtcgc ccccgccgcc cgcgccgacc aggtgcgcca
     1621 ggccgtcgcc gccgaccttg cgcg
//
LOCUS       DASVOR010002395         2406 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_84880,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002395 DASVOR010000000
VERSION     DASVOR010002395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84880"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..206)
                     /locus_tag="VF516_44080"
     CDS             complement(<1..206)
                     /locus_tag="VF516_44080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114812.1"
                     /translation="MSATPANPQPGPAPFRLALTREVTCIRSLAWRTDLATAVDWQRL
                     GAALEAAGLVRRPPVGAMQRFATAR"
     gene            complement(220..1473)
                     /locus_tag="VF516_44085"
     CDS             complement(220..1473)
                     /locus_tag="VF516_44085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114813.1"
                     /translation="MPIEDLRELLAEQSLTFLAGIPYVYHCHHYNLFHDQTIDDALGE
                     TEGARVRTIAAHNAFRELLGAVFAATGATTPAERISLAAEVFAWMGQGRISLDVSSSG
                     GRARSPSLHYGFAWREKYGAKVKRDEPTDAVAAGFAAATNELAFDLAAEAITATETRC
                     LAARDPECEFTLTPGKGHLSRPPIDEAAVRRYVIPAIRGLDEDRVAAISAGLLEFMRA
                     VQGDERGLVAAFNVFVTAHLPGYYNETVYEAVHHIERTAPAGAAAIEALMREAGQVCV
                     FNTFGNVLLSPEWEGMVGPLSGVPTDMMSFCTAIARGLGFGHWLIHEYEPHRRLVIQA
                     TSNYEVPFYRARYGVAKKPRSYFVQGAALAMMLLSETVPWTERPQLTNAYYIDLFRGK
                     PRYRVEQTRCMSRGDQLCEYVVEAL"
     gene            complement(1485..2060)
                     /locus_tag="VF516_44090"
     CDS             complement(1485..2060)
                     /locus_tag="VF516_44090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114814.1"
                     /translation="MDRYIEQAVAEIPQLIGIFVTAMPDSLLFSSWVREGTSWSAEDV
                     AAYFGDLVRANREGLKALGSWSSEMQVTIESAESLVVLRELSSDFVAGCVFERSAPLG
                     MVRLHLKRLLERITHALPKIQAEDRPRGLRIMEFLDRYAPDPHAVALRVSLRAGISLD
                     DLKMPGSLTPDQVQRLEETACKILGVKQLSL"
     gene            complement(2060..>2406)
                     /locus_tag="VF516_44095"
     CDS             complement(2060..>2406)
                     /locus_tag="VF516_44095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114815.1"
                     /translation="AWIEDCVPCRAAFIADDNGLPVVEHVGAEAGHVAAASSILMMLA
                     SVRTLMRDSGGWLSLQLGAQVLNVVEVATRWGRFGIGVVTEDALPRDFLLALSDAVER
                     AFHGEPSPGEAT"
BASE COUNT          367 a          837 c          857 g          345 t
ORIGIN      
        1 cgggcggtgg cgaaccgctg catcgcgccg accggcggcc ggcggaccag cccggcggcc
       61 tcgagcgcgg cgcccagccg ctgccagtcg accgcggtcg ccagatcggt gcgccacgcg
      121 agcgaccgga tgcacgtgac ctcgcgggtc agcgcgaggc ggaacggcgc aggtccgggc
      181 tggggattcg caggcgtcgc cgacatcgca gacatcccat cacagcgctt ccacgacgta
      241 ctcgcacagc tggtcgccgc gcgacatgca gcgggtctgc tcgacccggt agcgcggctt
      301 gccgcggaac agatcgatgt aataggcgtt ggtcagctgc ggccgctcgg tccacgggac
      361 ggtctcgctc agcagcatca tcgccagcgc cgcgccctgc acgaagtacg agcgcggctt
      421 cttggccacg ccgtagcgcg cgcgatagaa cggtacctcg tagttgctgg tcgcctggat
      481 cacgaggcgc ctgtgcggct cgtactcgtg gatcagccag tggccgaacc cgaggccgcg
      541 cgcgatcgcg gtgcagaacg acatcatgtc ggtggggacg cccgacagcg ggccgaccat
      601 gccctcccac tccggcgaca gcagcacgtt gccgaacgtg ttgaacacgc agacctggcc
      661 ggcctcgcgc atcagcgcct cgatggcggc cgcgccggcg ggggcggtgc gctcgatgtg
      721 gtgcaccgcc tcgtacacgg tctcgttgta gtagcccggc aggtgggcgg tgacgaacac
      781 gttgaacgcg gcgaccaggc cgcgctcgtc gccctggacc gcgcgcatga actcgagcag
      841 gccggcggag atcgcggcga cgcggtcctc gtcgaggccg cggatcgcgg ggatgacgta
      901 gcgccgcacg gcggcctcgt cgatcggcgg ccggctgagg tggcccttgc ccggggtcag
      961 cgtgaactcg cactcggggt cgcgggccgc caggcagcgg gtctcggtgg cggtgatcgc
     1021 ctcggccgcg agatcgaacg cgagctcgtt ggtcgccgcc gcgaagccgg ccgcgaccgc
     1081 gtcggtcggc tcgtcgcgct tgaccttggc gccgtacttc tcgcgccagg cgaagccgta
     1141 gtgcagcgat ggcgagcgcg cccggccgcc ggagctcgac acgtcgagcg agatccggcc
     1201 ctggcccatc cacgcgaaca cctcggcggc caggctgatc cgctcggccg gggtggtcgc
     1261 gccggtcgcc gcgaacacgg cgccgagcag ctcgcggaac gcgttgtggg ccgcgatggt
     1321 gcggacccgg gcgccctcgg tctcgccgag cgcgtcgtcg atggtctggt cgtggaacag
     1381 gttgtagtga tggcagtgat acacgtaggg aatgcccgcc agaaacgtca ggctctgctc
     1441 ggcgagcaat tctctcaggt cttcgatcgg catgtcctgt cctttcacag actcagctgc
     1501 ttgacgccga ggatcttgca ggcggtctcc tccagccgct ggacctgatc gggtgtcagc
     1561 gagccgggca tcttgagatc gtcgagcgag atcccggcgc gcagcgacac acgcagcgcc
     1621 accgcatgag gatccggggc atagcgatcg aggaactcca tgatgcgcag cccgcgcggc
     1681 cggtcctcgg cctggatctt gggcagcgcg tgggtgatgc gctcgagcag gcgcttgagg
     1741 tggagccgga ccatgccgag cggcgccgag cgctcgaaca cgcagccggc gacgaagtcg
     1801 ctcgacagct cgcgcagcac gacgagcgac tcggcggact cgatcgtcac ctgcatctcg
     1861 ctcgaccacg agccgagcgc cttgagcccc tcgcggttgg cgcggaccag atcgccgaag
     1921 tacgccgcga cgtcctccgc gctccacgac gtgccctcgc gtacccacga cgagaacagc
     1981 aggctgtccg gcatcgccgt cacgaagatc ccgatcagct ggggaatctc cgccactgct
     2041 tgttcgatgt agcggtccat tacgttgctt ctcctggaga cggttctccg tgaaaggcgc
     2101 gctcgacggc gtcgctcagc gccagcagga agtcgcgcgg cagggcgtcc tcggtcacca
     2161 cgccgatccc gaaccggccc cagcgggtcg cgacctcgac cacgttgagc acctgggcgc
     2221 cgagctggag cgacagccag ccgccgctgt cgcgcatcag cgtgcggacc gaggcgagca
     2281 tcatgaggat cgacgacgcg gcggcgacgt ggccggcctc ggccccgacg tgctcgacga
     2341 ccggcaggcc gttgtcgtcg gcgatgaacg cggcgcggca cggcacgcag tcctcgatcc
     2401 acgcga
//
LOCUS       DASVOR010002396         4710 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_230938,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002396 DASVOR010000000
VERSION     DASVOR010002396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4710
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230938"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1700
                     /locus_tag="VF516_44100"
     CDS             195..1700
                     /locus_tag="VF516_44100"
                     /inference="COORDINATES: protein motif:HMM:NF014218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HEX8114816.1"
                     /translation="MAESSEILHGDGRVELRAPDQLEGSPLYNADLAPVPVARRTWTT
                     WDYAALWISMAHCIPTYTMASGLIDSGMSWWQALGTILLGNLIVLAPILLNSHPGTKY
                     GIPFPVFARAAYGTFGSNVPALMRALVACGWFGINAWIGGAALHKFLVSLAPGWQDLL
                     GTVHGSPGTQWIAFLLFWALNILIIYRGMEVVRRVERWAAPFVLVMTAILLVWAIGRA
                     DGLGPIMARPGTLTTSRAFLAVFVPSLTAAIGFWSTLSLNMPDFTRFGRSQRAQAIGQ
                     AVALPTTMTVFAGMGIMITSATVLIFGKAISDPIELGGRFDNRLIVGVVMFTAVIATL
                     AVNIAANVVSPANDFANAFPRHIDFKRGGLITGVLGIAAMPWELLKNAGRYIGWLVGY
                     SGSLGAIAGVLIVDYWLLRRRTLDLTSLYVRDGAYRYRDGYNLAAIGATLVGAAVALA
                     GIFWDPLRPIYDWSWFVGFGLAGALYWALMQGRVPPSPHPGSARADIAPPR"
     gene            1831..2673
                     /locus_tag="VF516_44105"
     CDS             1831..2673
                     /locus_tag="VF516_44105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114817.1"
                     /translation="MTTDRFAPGARIGDYVVEREVSYEAAAIVYFATHVVLPRKSHVK
                     VSHPGSRPAAVQLLREACILEALSHPGAPRVYECGVLADRRPWSSIERLPGVAFDRFV
                     GDRPAALSDVVVALRDVADVLRHAHERGIVHRRLTASAILRTERRRSVYAIEDWADAR
                     TLDAEAGVTVDAREDIVALGAIVFRALTARDPAPGVSLATHCSAAPTELISLIDQMLA
                     EPVARPTAGEIYERALWLCETLEASPLFERPRWTPPRGFVSEAASPNESGGFSVKIGR
                     DPTR"
     gene            2756..3799
                     /locus_tag="VF516_44110"
     CDS             2756..3799
                     /locus_tag="VF516_44110"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8114818.1"
                     /translation="MTQVLDVGFSPDDRIGNYRVEHELGPTGSGILLQARHQVLPRRA
                     ILKVVHAAFAGESPYLVQTLREACILEAISHPGVPIVYESGLLQDRRPWFAFELIHGP
                     TLEDLLVPRALPIREVARVMRDLADILEHAHRRGVIHRGLRPDRIVITRHRRYPLCIP
                     DWSEAIAHDASASVPPPDGSRAYVAPELAHQDAGGAERVDDRADMFALGAIAYRALTG
                     TVPFNPVAAKVPYLAARTRRPDTPRELATIVDSLLSVDRFDRPSASEVRAELDWLVGT
                     LPEPPQHVAPRAPDPDEGAVAEGSVPPVATSAKADVALSPPRVRRPRWTPTVHYVEST
                     DVDILIDEDSPAG"
     gene            3927..>4710
                     /locus_tag="VF516_44115"
     CDS             3927..>4710
                     /locus_tag="VF516_44115"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8114819.1"
                     /translation="MVHAPSGVPGVRIADYWLHDELPARSTEVAYRATHRVLPRSARI
                     AILKSEHVGVRHAEIQLMREACVVETLHHAGVPRVYECGVLDRRPWVASEQIAGTSIE
                     EVAAKRPLPIGEAIAVVRDAAAILAHAHRRGVIHGDLTPRSILRTAQRGFPVCITDWG
                     SAALYGTALPRSDDPAGRFYRAPEVTGSDRGGSAADVFALGAVMFEAATLVLPEPVQK
                     FPGMPPAFHQLLARMLAPAADDRPTADAVHAEAARLAELFSDA"
BASE COUNT          640 a         1702 c         1597 g          771 t
ORIGIN      
        1 gatggtctcg caggtcctga ccctgttcac gacgccggtg acctacctgg cgatgggccg
       61 gttcgccgcg tggctgcgcc gggcgctcgg ccgcggcccg cgcgacgtcg gctcgacgat
      121 cctgccgcgc tagcgaaccg ctgcttgcga tcggatctcg cttcacaccg cggaatcgac
      181 taggttcgac ggcgatggcc gagtcgagcg aaatcctgca cggggacggc cgggtcgagc
      241 tgcgcgcacc ggaccagctg gagggatcgc cgctgtacaa cgccgacctc gcgccggtgc
      301 cggtcgcgcg ccggacctgg acgacctggg actacgccgc gctgtggatc tcgatggcgc
      361 actgcatccc gacctacacg atggcgtcgg ggctgatcga cagcgggatg agctggtggc
      421 aggcgctcgg cacgatcctg ctcggcaacc tgatcgtgct cgccccgatc ctgctcaact
      481 cccaccccgg gaccaagtac ggcattccgt ttccggtatt cgcgcgcgcg gcctacggca
      541 cgttcggctc caacgtgccg gcgctgatgc gcgcgctggt cgcgtgcggc tggttcggga
      601 tcaacgcgtg gatcggcggc gccgcgctgc acaagttcct ggtctcgctc gcgccgggct
      661 ggcaggacct gctcggcacg gtgcacggtt cgccgggcac ccagtggatc gcgttcctgc
      721 tgttctgggc gctcaacatc ctgatcatct accgcggcat ggaggtggtg cgccgggtcg
      781 agcgctgggc cgcgccgttc gtgctggtga tgaccgcgat cctgctggtc tgggcgatcg
      841 gccgcgccga cgggctcggc ccgatcatgg cgcggccggg cacgttgacc accagccgtg
      901 cgttcctcgc cgtgttcgtg ccgtcgctga ccgcggcgat cgggttctgg tcgacgctgt
      961 cgctcaacat gcccgacttc acgcggttcg gccggtcgca gcgcgcgcag gcgatcggcc
     1021 aggcggtggc gctgccgacg acgatgaccg tgttcgccgg gatgggcatc atgatcacct
     1081 cggcgaccgt gctcatcttc ggcaaggcga tctccgaccc gatcgagctc ggcggccggt
     1141 tcgacaaccg gctgatcgtc ggcgtcgtga tgttcaccgc ggtgatcgcg acgctcgcgg
     1201 tcaacatcgc cgccaacgtg gtgtcgccgg ccaacgactt cgccaacgcg ttcccgcgcc
     1261 acatcgactt caagcgcggc ggcctgatca ccggcgtgct cggcatcgcc gcgatgccgt
     1321 gggagctgct caagaacgcc ggccgctaca tcggctggct ggtcggctac tcgggctcgc
     1381 tcggcgcgat cgcgggcgtg ctgatcgtcg actactggct gctgcgccgg cgtaccctcg
     1441 atctcacgtc gctctacgtc cgcgatggcg cctatcgata ccgcgacggc tacaacctcg
     1501 ccgcgatcgg cgcgacgctg gtcggcgccg cggtcgcact cgccgggata ttctgggacc
     1561 cgctgcgccc gatctacgac tggtcctggt tcgtcggctt cggcctggcc ggcgcgctgt
     1621 actgggcgct gatgcagggc agggtcccac cttcaccaca cccgggcagc gctcgggccg
     1681 atatcgcacc tccgcgctga cgtccgctgt ccggacggcg gacaatcgaa cctggattcg
     1741 tgccactatc ccgtcagatg ctgtgatgga gatcgacgtt cttcctcggg gcatgaacac
     1801 tgcacctcgc gggtggccat gaccccgtcc gtgaccacag atcgcttcgc gccgggcgcg
     1861 cggatcggcg actacgtcgt cgagcgcgag gtctcgtacg aggccgccgc gatcgtctac
     1921 ttcgcgaccc atgtcgtgct gccgcgcaag tcgcacgtca aggtcagcca tccgggctcg
     1981 cgccccgccg cggtccagct gctgcgcgag gcctgcatcc tcgaggcgct gtcccatccc
     2041 ggcgcgccgc gcgtctacga gtgcggcgtg ctcgccgatc gccggccgtg gagctcgatc
     2101 gagcggctgc cgggcgtcgc gttcgatcgc ttcgtcggcg atcgccccgc cgcgctgtcc
     2161 gacgtcgtcg tcgcgctgcg cgacgtcgcg gacgtcctgc gccacgccca cgagcgcggc
     2221 atcgtgcatc gccggctgac cgcgagcgcg atcctgcgca ccgaacgccg gcgcagcgtc
     2281 tacgcgatcg aggactgggc cgacgcgcgc acgctcgacg ccgaggcagg cgttaccgtc
     2341 gacgcgaggg aggacatcgt cgcgctgggc gccatcgtct ttcgcgccct caccgcgcga
     2401 gacccggcgc cgggcgtctc gctcgcaact cactgttcag cagcgcccac tgaactgatc
     2461 tccctgatcg atcagatgct cgccgagcct gtcgctcggc cgaccgcggg cgagatctat
     2521 gaacgagcac tctggctctg cgagaccctc gaagcctccc cgctgttcga gcggccccgc
     2581 tggaccccgc cacgcggctt cgtttctgaa gctgcttcgc ctaatgaatc cggtggcttt
     2641 tccgtcaaga tcggccgaga tccgacccgc tagagctcac tcacgatccg cgcggcaggt
     2701 cgcgaaaaaa actgttccac agcggccgat atttcggtaa tgtaatacac aagccatgac
     2761 gcaggtgttg gatgtaggat tcagccctga cgaccggatc gggaactacc gtgtcgagca
     2821 tgagcttggg cccacgggtt cgggcatcct gctccaggcg cggcatcagg tattgccgcg
     2881 tcgggcgatc ctcaaggtgg tgcacgcggc gtttgccggg gaatctccgt atctggttca
     2941 gaccctgcgg gaggcctgca tcctcgaggc gatcagccat cccggcgttc cgatcgtgta
     3001 cgagtcgggg ctgttgcagg atcgccggcc gtggttcgcg ttcgagctga tccacggacc
     3061 gacgctggag gatctgctcg tgccccgcgc acttccgatc cgcgaggtcg ctcgcgtgat
     3121 gcgcgatctc gccgacatcc tggagcatgc gcaccgccgc ggcgtcatcc accgcggcct
     3181 gcggcccgac cggatcgtga tcacccgtca ccggcgatac ccgctgtgca tcccggactg
     3241 gagcgaggcg atcgcacacg acgccagcgc cagcgtgccg ccgcccgacg gcagccgcgc
     3301 ctacgtcgcc cccgagctcg cccaccagga cgcgggcggc gccgagcggg tcgacgatcg
     3361 cgccgacatg ttcgcgctcg gtgcgatcgc gtaccgcgcg ctgaccggca cggtgccgtt
     3421 caacccggtc gccgcgaagg tgccgtatct cgcggcacgc acccggcgcc ccgatacgcc
     3481 gcgcgagctg gcgacgatcg tcgattcgct gctgtcggtc gaccgcttcg accggccgag
     3541 cgcctccgag gtccgcgccg agctcgactg gctggtcggc acgctgccgg agccgccgca
     3601 acatgttgcg ccccgcgcgc cggatcccga cgagggtgcc gtggccgagg gcagtgtccc
     3661 gccggtggcg acgagcgcga aggccgacgt cgcgctcagc ccgccgcgcg tgcgccggcc
     3721 gcggtggact ccgacggtgc attacgtcga gagtactgac gtcgatatcc tcatcgacga
     3781 ggattccccg gcgggttagg cgcgctaggg actaaaggac ggggtcaacg tgcccgaggt
     3841 ctgaagaccc gaggccgacg aggggtcccc gtgctgtgca gtcacagtgt tggaagaggc
     3901 acactcactg cactgtaggt tttgtaatgg tacatgcgcc gtcgggtgtc ccgggagtgc
     3961 ggatcgccga ctactggctc cacgatgagc tccccgcccg cagcaccgag gtcgcctatc
     4021 gcgcgaccca tcgcgtgctt ccccgatcgg cccggatcgc gatcttgaag tccgagcacg
     4081 tcggtgtccg gcacgccgag atccagctga tgcgcgaggc gtgcgtggtg gagaccttgc
     4141 accacgccgg cgtgccgcgg gtctacgagt gcggcgtgct cgaccgccgg ccgtgggtcg
     4201 cgagcgagca gatcgccggc acctcgatcg aggaggtcgc cgccaagcgc ccgcttccga
     4261 tcggcgaagc gatcgcggtc gtccgcgacg ccgccgcgat cctcgcccac gcccaccgcc
     4321 gcggcgtcat ccacggcgac ctcacaccgc ggtcgatcct gcgcaccgcg cagcgcggct
     4381 ttccggtgtg catcaccgac tggggcagcg cggcgctcta cggcaccgcg ctgccgcggt
     4441 ccgacgaccc ggccggccgg ttctaccgcg cgcccgaggt caccggcagc gatcgcggcg
     4501 gctcggccgc cgacgtgttc gcgctcggcg cggtgatgtt cgaggccgcg accctggtcc
     4561 tgcccgagcc ggtccagaag tttcccggca tgccgccggc gttccaccag ctgctcgcgc
     4621 gcatgctcgc gccggctgcc gacgaccggc cgaccgccga cgccgtgcac gccgaggccg
     4681 cccggctcgc cgagctgttc agtgacgccg
//
LOCUS       DASVOR010002397         6106 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_405200,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002397 DASVOR010000000
VERSION     DASVOR010002397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405200"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="VF516_44120"
     CDS             complement(<1..1076)
                     /locus_tag="VF516_44120"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEX8114820.1"
                     /translation="MTRSLTALLLAAVSLSTRSAGAEKYSRADVMKRAARHPTVEAAN
                     SQVDQVRAQKRQADALRWPRISLSLGVLPSLRATLAPGSQVDSVERATDYKLDDVRPG
                     FTGDLTIIQPLYTFGKIGQRQRAAELGVGAQTAQARMRQADVAVEGADLYEKYLLARD
                     LERFLEDTDHMLVRSIEATEDRLAARAADVSQHDLLRLKTARGPLIVGLHQAQAGTRQ
                     AEAGLRAYFGLRDGTPIEPADARLEAIPAGKLALAELVRRAYRDRPELVALADGAGAF
                     DALAQAERSGYLPDIVALGFVSGAYTVDRDAVESRFVYDPARHFVPGIGLGLRWELWG
                     DLAGGRADEQRARGAELRHLERWAR"
     gene            complement(1084..1596)
                     /gene="mlaD"
                     /locus_tag="VF516_44125"
     CDS             complement(1084..1596)
                     /gene="mlaD"
                     /locus_tag="VF516_44125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEX8114821.1"
                     /translation="MANHSRLEISVGAFVAAGFAIVAYLAISLGNVRLLSGHQTTLVA
                     RFSSIGDLRSAAPVRIAGVHVGQVGKIQLADYVARIELAIDSDVQIPSDTIASIRTSG
                     LLGESFVSLSPGGSDRILRDGDAIVQTEPALDITQLLQKYAFGSTTEPSSAKDEGRGR
                     SEPGPQDPFR"
     gene            complement(1602..2408)
                     /locus_tag="VF516_44130"
     CDS             complement(1602..2408)
                     /locus_tag="VF516_44130"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEX8114822.1"
                     /translation="MLELHHLTQGLRPTNMLGGLGRRVIEGLGGLGRFVVFSGRAAVA
                     IGRRPFRLHVVVEQLHFFGNRSIAIAVLTASFTGMVLALQGHDTLQRFGSDSQVGPLV
                     ALSLLRELAPVLAGIVIAARAGSATAATLGNMRMTEQIDAIEVMAIDSRQYLVSTRLL
                     AAMIAVPIITALFSLAGIVAAFVLATQVLGQEPGVFWGGVERSVEWADISLGLEKSFV
                     FAALIATISCYRGYHATGGATGVGRATTRAVVEATSLILVGDYILTALLM"
     gene            complement(2548..4899)
                     /gene="ppsA"
                     /locus_tag="VF516_44135"
     CDS             complement(2548..4899)
                     /gene="ppsA"
                     /locus_tag="VF516_44135"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: protein motif:HMM:NF005057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEX8114823.1"
                     /translation="MTSVRWFRELGLADIATVGGKNANLGELVRHLAPLGVRVPDGFA
                     LVADAFRRHLREARLDGEIHAALDQLDVRDVAALARTGRMIRERVCAAALPADIEAEL
                     RAAYDALSRQFGEPATDVAVRSSATAEDLPDASFAGQQESYLNVHGHAALIDAVKRCM
                     ASLFTDRAIVYRVERGYAHRAVALSVGVQKMVRSDLASAGVMFTLDTESGFRDVIEIT
                     GAWGLGETVVKGEVNPDEITVHKPTLAQGFRPILRRDAGDKAIKLIYADGGNGATRQV
                     PVTAADRARLVLSDDEALALARWGIAIEQHYDTAMDIEWAKDGRSGELYIVQARPETV
                     HTQRHSPALELYRIKGKGELRLTGKSVGARIATGRARVIHDASELASFVDGEVLVAAM
                     TDPDWEPVLARAAAVVTDQGGRTCHAAIVSRELGIPCVVGTVVATRTLATGDVVTVSC
                     AEGTEGHVYAGQVPFACEVIDPATLPTPTVPVMLNVGNPENAFQLGRLPSAGVGLARI
                     EFIISAQIGIHPMALLHPERVTDAWAVAVIRGRLADADPKEFFIDRLATGVARIAAAF
                     WPRPVIVRFSDFKTNEYANLLGGAAFEPVESNPMIGFRGASRYYDPRYREGFALECAA
                     IKRVREVMGLGNVKVMIPFCRTLGEARKVLNEMTANGLTQASGLEVYVMCEIPNNVVL
                     AAEFAALFDGFSIGSNDLTQLTLGVDRDSELLAPLFDEQDPGVKALIAQVVHVAHAHG
                     RKVGLCGQAPSDHPEFAGFLASIGIDSISVTPDALAAVVKTLA"
     gene            5185..>6106
                     /locus_tag="VF516_44140"
     CDS             5185..>6106
                     /locus_tag="VF516_44140"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8114824.1"
                     /translation="MRPVPLLALLALLGIRTSARADDCERIAIWRDGHAVGERCRGDA
                     SEHELAILDLSDDWVPPVFAADEDSGPGYRPIYLALAQERFADAGGDAALAPADRYFE
                     LFGIAPSLGVVRARLGDEARHRCHAAIDDAPLAAAPERIIGETGAQAAARIASARGLR
                     AELEHDRARRKLADLEALAAVDAYYRRAVDRLTALEAYLAAVRTLQAHLECDGLFTSP
                     PADEAYTWQTSEAVARFQRGAMILPTGIVDAATRDALALGSRERDFRTALRVLRERVA
                     AATGLIEDGTAGTGPRTVLGRELEPEATWRV"
BASE COUNT          901 a         2166 c         2182 g          857 t
ORIGIN      
        1 cgcgcccagc gctcgaggtg ccgcagctcg gcgccccgtg cgcgctgctc atcggcgcgc
       61 ccacccgcga ggtcgcccca cagctcccag cgcagcccga ggccgatccc gggaacgaag
      121 tgacgggccg gatcgtagac gaaccggctc tccaccgcat cgcggtccac ggtgtacgca
      181 ccggacacga agccgagcgc cacgatgtcc ggcaggtagc cagagcgctc cgcctgcgcc
      241 agcgcgtcga acgccccggc gccgtccgcc agcgcgacga gctcgggtcg atcgcggtac
      301 gcgcgccgga cgagctcggc cagcgcgagc ttgcccgccg ggatcgcctc gaggcgcgcg
      361 tccgccggct cgatcggcgt tccgtcgcga agcccgaagt acgcgcgcag cccggcctcg
      421 gcctgtctgg ttccggcctg ggcttgatgg agaccgacga tcagcgggcc gcgtgcggtc
      481 ttgaggcgca acagatcgtg ctggctgacg tccgctgccc tggcggcgag gcgatcctcg
      541 gtcgcctcga tcgagcgcac gagcatgtgg tcggtgtcct cgaggaagcg ctcgaggtcg
      601 cgcgccagga ggtacttctc gtacaggtcg gccccctcga ccgcgacgtc cgcctgccgc
      661 atccgggctt gcgcggtctg cgcgccgacc cccagctcgg ccgcgcgctg gcgctgaccg
      721 atctttccga acgtgtagag cggctggatg atcgtcaggt ctcccgtgaa gccgggcctc
      781 acgtcgtcga gcttgtagtc ggtggcccgc tcgaccgagt ccacctgcga gcccggcgcc
      841 agggtggcgc ggagcgacgg aaggactccg agcgacagcg agatccgcgg ccagcggagc
      901 gcgtccgcct gccgcttctg ggcccggacc tggtcgacct ggctgttcgc cgcctcgacg
      961 gtcgggtggc gcgcggcacg cttcatcacg tcggcgcgag agtacttctc cgcgccggcc
     1021 gatcgcgtcg acagcgatac ggcggcgagc aacagagcgg tgagcgatcg tgtcatggtg
     1081 tcctcatcgg aatggatcct gtggaccggg ctcggaccga ccacggccct cgtccttggc
     1141 cgacgacggc tcggtggtgg aaccgaatgc gtacttctgg agcagctggg tgatgtcgag
     1201 cgccggctcg gtctgcacga tggcgtcgcc gtcgcggaga atccgatcgc tcccgcccgg
     1261 cgacagcgag acgaatgact cgccgagcag accgctggtc cggatcgagg cgatcgtgtc
     1321 ggacgggatc tggacgtcgg agtcgatcgc gagctcgatg cgcgcgacat agtcggcgag
     1381 ctggatcttg ccgacctggc cgacatggac cccggcgatc cggaccggcg ctgcgctgcg
     1441 caggtcgccg atcgacgaga accgggcgac gagtgtcgtc tggtgtccgg atagcaagcg
     1501 cacattgccg agcgagatcg ccagataggc gacgatcgcg aagccggccg ccacgaaggc
     1561 gccgaccgag atctcgagtc gtgagtgatt cgccatgatg cctacatgag cagtgcggtg
     1621 aggatgtagt ccccgacgag gatgagcgag gtggcctcga ccacggcacg cgtggtcgct
     1681 cgcccgacgc cggtcgcgcc accggtcgcg tggtacccgc ggtagcagct gatggtcgcg
     1741 atgagcgcgg cgaacacgaa ggacttctcg agcccgagcg agatgtccgc ccattcgacc
     1801 gagcgctcga ccccgcccca gaacacgccg ggttcctggc cgagcacctg ggtcgcgagc
     1861 acgaacgcgg cgacgatgcc ggccaggctg aacagcgcgg tgatgatcgg gaccgcgatc
     1921 atcgccgcca ggaggcgcgt gctgaccagg tactgccgcg agtcgatcgc catcacctcg
     1981 atcgcgtcga tctgctcggt catgcgcatg ttgccgagcg tcgccgcggt cgccgagccg
     2041 gcccgcgcgg cgatgacgat tcccgccagg accggcgcga gctcacggag caagctcagc
     2101 gcgaccagcg gaccgacctg ggaatcgctg ccgaagcgct gcagggtgtc gtgcccctgg
     2161 agcgccagca ccatcccggt gaagctggcg gtgaggacgg cgatggcgat cgagcggttg
     2221 ccgaagaagt ggagctgctc gaccaccacg tggagacgaa acggacggcg gccgatggcg
     2281 acggcggccc ggccggagaa caccacgaac cgaccgagac cgcccaggcc ctcgatcaca
     2341 cgacggccga gaccgccgag catgttcgtc ggtcgcagcc cttgcgtgag atgatggagc
     2401 tcgagcatgg ccgtcacttc ccagtcgtgc gatcgcctgc atgccctcgc tgcatccgtc
     2461 attccaggac ggttcgaggc ccgccgcgtc tcggccgcgc aaactcggtc gatacgagcc
     2521 gatcactcgc aagatctgcg cgtcctccta cgcgagcgtc ttcaccaccg cggccagcgc
     2581 gtcgggggtg accgagatcg agtcgatccc gatcgacgcg aggaagcccg cgaactcggg
     2641 gtgatcgctg ggcgcctggc cgcacagccc gaccttgcgg ccatgcgcgt gggcgacgtg
     2701 gacaacctgc gcgatcagcg ccttgacacc cggatcctgc tcgtcgaaca gaggggcgag
     2761 cagctccgag tcgcggtcga cgccgagcgt cagctgcgtc aggtcgttcg agccgatcga
     2821 gaacccgtcg aacagcgcgg cgaactccgc cgcgagcacg acgttgttcg ggatctcgca
     2881 catcacgtac acctcgaggc cgctcgcctg cgtcaggccg ttggccgtca tctcgttcag
     2941 caccttgcgg gcctcgccga gcgtgcggca gaacgggatc atcaccttga cgttgcccag
     3001 gcccatgacc tcgcggaccc gcttgatcgc ggcgcactcg agcgcgaagc cctcgcggta
     3061 gcgcggatcg tagtaacgcg acgcgccgcg gaacccgatc atcgggttgg actcgaccgg
     3121 ttcgaacgcc gcgccgccga gcaggttggc gtactcgttg gtcttgaagt ccgagaaccg
     3181 gacgatcacc ggccgcggcc agaacgccgc cgcgatccgg gcgacgccgg tcgccagccg
     3241 gtcgatgaag aactccttgg gatcggcgtc ggcgagccgg ccgcggatca ccgccaccgc
     3301 ccacgcgtcg gtgacgcgct cgggatggag cagcgccatc ggatggatcc cgatctgggc
     3361 cgagatgatg aactcgatcc gcgccaggcc gacgcccgcg ctcggcagcc ggccgagctg
     3421 gaacgcgttc tccgggttgc cgacgttgag catcaccggc acggtcggcg tgggcagggt
     3481 cgccggatcg atcacctcgc aggcgaacgg cacctgcccg gcgtagacgt ggccctcggt
     3541 gccctcggcg cacgacacgg tgacgacgtc gccggtcgcc agcgtccggg tcgcgacgac
     3601 ggtgccgacg acgcatggga tcccgagctc gcgcgacacg atcgcggcgt ggcaggttcg
     3661 cccgccctgg tcggtcacga ccgccgccgc ccgcgccagc accggctccc agtcgggatc
     3721 ggtcatcgcg gcgaccagga cctcaccgtc gacgaagctc gccagctccg acgcgtcgtg
     3781 aatcacgcgc gcgcggccgg tcgcgatccg ggcgccgacg ctcttgccgg tcagccggag
     3841 ctcgcccttg cccttgatcc ggtagagctc cagcgccggg ctgtgccgct gggtgtgcac
     3901 ggtctcgggc cgcgcctgca cgatgtagag ctcgccggac cggccgtcct tggcccactc
     3961 gatgtccatc gcggtgtcgt agtgctgctc gatcgcgatg ccccagcgcg cgagcgcgag
     4021 cgcctcgtcg tcggacagca ccagccgcgc gcgatcggcg gcggtgaccg ggacctggcg
     4081 ggtcgcgccg ttgccgccgt cggcgtagat cagcttgatc gccttgtcgc cggcgtcgcg
     4141 gcgcaggatc ggccggaagc cctgggccag ggtcggcttg tgcaccgtga tctcgtcggg
     4201 gttgacctcg cccttgacca cggtctcgcc caggccccac gcgccggtga tctcgatgac
     4261 gtcgcggaac cccgactcgg tgtcgagcgt gaacatcacg ccggccgacg cgagatcgct
     4321 gcgcaccatc ttctggacac cgaccgacag cgcgaccgcg cgatgcgcgt agccgcgctc
     4381 gacgcgatag acgatcgcac gatcggtgaa cagcgacgcc atgcagcgct tgaccgcgtc
     4441 gatcagcgcc gcgtggccgt ggacgttgag gtagctctcc tgctggccgg cgaacgatgc
     4501 gtcggggaga tcctcggcgg tcgcgctcga tcgcaccgcg acgtcggtcg ccggctcgcc
     4561 gaactgccgc gacagcgcgt cgtacgcggc gcgcagctcg gcctcgatgt cggctggcag
     4621 cgccgcggcg cacacccgct cgcggatcat ccggccggtg cgcgcgagcg ccgcgacgtc
     4681 ccggacgtcg agctggtcga gcgcggcgtg gatctcgccg tcgagccgcg cctcgcggag
     4741 gtggcgccgg aacgcgtccg cgacgagcgc gaagccgtcg ggcacgcgca cgccgagcgg
     4801 cgcgaggtgg cggaccagct cgccgaggtt ggcgttcttg ccgccgaccg tggcgatgtc
     4861 ggccaggccg agctcgcgga accagcgcac cgacgtcatg gcgccacccc ctcgcgctcg
     4921 ggtggctcgg tcgtcgtcgc ctcgctggat tgaccggtgg aatccctcat tcgccgcagc
     4981 atgagcatgc ggtgtgccag tggtggatgg atctcgaggc ccgacgcggc gcaagtactt
     5041 cggccgcgcg ccgcgccggc gcaaggaatg cgcgaaccac gcggaggtga ccgcgccacc
     5101 aggactgact cgcagtgatg tgcaggacca tgagcgggtt gccggcacgc gatcctggca
     5161 cgaccgctgc tcggtagcgg aagtgtgcgt ccagtccccc tgctcgccct gctcgccctg
     5221 ctcggaatac gaacgtccgc ccgcgcggac gactgcgagc ggatcgcgat ctggcgagac
     5281 ggccacgcgg tcggggagcg gtgtcgcggt gacgccagcg aacacgagct tgcgatcctc
     5341 gatctatcgg acgactgggt tccaccggtg ttcgcggccg acgaggacag cgggccgggc
     5401 tatcgaccga tctacctcgc gctcgcgcag gagcggttcg cagacgccgg cggggacgct
     5461 gcgctcgcac cggcggaccg ctacttcgag ctgttcggca tcgctccgag cctgggcgtc
     5521 gtgcgggcgc ggctcggcga cgaggcccgc catcgctgcc acgccgcgat cgacgatgcg
     5581 ccgctggccg ctgctcccga gcggatcatc ggggagaccg gcgcacaggc cgcggcgcgg
     5641 atcgcgagcg cgcgcgggct gcgcgccgag ctggagcatg accgcgcgcg acgcaagctg
     5701 gccgatctcg aggcgctggc cgccgtcgac gcctactacc ggcgggcggt cgatcgcctg
     5761 accgcgctgg aggcgtatct cgccgcggtg cggaccctcc aggcccacct ggagtgcgat
     5821 ggcctgttca ccagcccgcc cgccgacgag gcgtacacct ggcagaccag cgaggcggtc
     5881 gcacggttcc agcgcggtgc gatgatcctg ccgaccggca tcgtcgacgc ggcgacgcgt
     5941 gatgcgctcg cgctgggcag ccgagagcgc gacttccgca cggcgctgcg cgtcctgcgc
     6001 gagcgggtgg ccgccgcgac cggcctgatc gaggacggca cggccgggac cgggccgcgc
     6061 accgtgctcg gccgcgagct cgagcccgag gcgacctggc gtgtgc
//
LOCUS       DASVOR010002398         2735 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_172342,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002398 DASVOR010000000
VERSION     DASVOR010002398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172342"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1266)
                     /locus_tag="VF516_44145"
     CDS             complement(<1..1266)
                     /locus_tag="VF516_44145"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HEX8114825.1"
                     /translation="MSDTEATRLRRAMAAVLALKQRNTDLEARIAALEGARCEPIAIS
                     AMACALPGHVRTPDELWQLLDAGRDAIGPFPARWAGLDLYDPDPDAAGKTYAREGGFV
                     TGVETFDPGLFGISPREAAAMDPQQRLALESAWEVLERAGVTSSAAVRARTGVYLGLN
                     ESDYALEERCDLGRLDGYHGTGNLGSVASGRISYALGLQGPSVTVSTACSSSLVAIHL
                     ACAALRASECDLALAGGVTVMSSPASFVEFSRMRASAPDGRCKSFSASADGAGWAEGA
                     GMLLLKRLGDALADGDRVLAVIRGSAIGQDGRSQGLTVPNGSAQEQVIRDALAASRLE
                     PHAIDAIEAHGTGTSLGDPIEANALARVFGPGRDRARPLHLGSVKSNLGHTQAAAGVV
                     GVIKMVLALAHERLPRSLHIEQLSPHVRWA"
     gene            complement(1315..2205)
                     /locus_tag="VF516_44150"
     CDS             complement(1315..2205)
                     /locus_tag="VF516_44150"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8114826.1"
                     /translation="MSAPEHTRRYLAAVNQQADLDAIPRATIEDIEIRYPDCTLAQAR
                     AIYDTWGCVVVRNLLPDHVAPILRDTEAMADAVRASGFEARHGALIRPGDSTGRAHQI
                     QLFYPTFMHAASTFRAAIDPRTADFLGALIGPNVEIHSEGMLIYKEPGGGMEKSMHQD
                     APYFYHRDHSFCTAFLHLVPTTQHNGRLHIIPGSHRLGLLEHHDRFSHMALPPDRYPL
                     SRALGIDGEPGDVVFFNYFIIHGSDRNWSDTPRPALIMQYRAAEDVQLWDSCAFTADV
                     GKLKNQKTSPKLLLCGRRAA"
     gene            complement(2241..>2735)
                     /locus_tag="VF516_44155"
     CDS             complement(2241..>2735)
                     /locus_tag="VF516_44155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114827.1"
                     /translation="SRTRELCGHRTCTMHELMDCDSSDFGSSPWKGPAFDPEGELAGQ
                     LPERYIVAYALGFAKLEYLSELDAPTRTAAAAAEATPGLLGYRMGASERCGGAATLSL
                     WASEEAMESFVYSDAHQQATMTVDHAFYGNGSTHYERTRDAGLPTFAEAEAHLRADLE
                     TTHK"
BASE COUNT          375 a          915 c         1033 g          412 t
ORIGIN      
        1 cgcccagcgg acgtgtgggc tgagctgttc gatgtggagc gatcgcggca accgctcgtg
       61 cgcgagcgcc agcaccatct tgatcacgcc gaccacgccg gcggccgcct gggtgtggcc
      121 gaggttcgac ttgaccgagc cgaggtggag cgggcgcgcg cgatcgcggc ccgggccgaa
      181 cacgcgggcg agcgcgttgg cctcgatcgg atcgccgagc gacgtgccgg tgccgtgagc
      241 ctcgatcgcg tcgatcgcgt gaggctcgag ccggctcgcg gcgagcgcgt cgcggatcac
      301 ctgctcctgc gccgacccgt tcggcacggt gagcccctgg ctgcgaccgt cctggccgat
      361 ggccgagccg cggatcaccg cgagcacgcg gtcgccgtcc gcgagcgcgt cgccgagccg
      421 cttgagcagg agcatgccgg cgccctcggc ccagcccgcg ccgtcggcgg aggccgagaa
      481 gctcttgcag cggccatcgg gcgccgaggc tcgcatccgc gagaactcga cgaacgacgc
      541 cgggctgctc atcaccgtga cgccgccggc gagcgcgagg tcgcactcgc tcgcgcgcag
      601 cgccgcgcag gccaggtgga tcgcgaccag ggatgacgag cacgccgtcg acaccgtgac
      661 cgaagggccc tgcaggccga gcgcatagga gatccggccc gacgcgaccg agccgaggtt
      721 gccggtcccg tggtagccgt cgagccggcc taggtcgcag cgctcctcga gggcgtagtc
      781 ggactcgttg agtccgaggt agacgccggt gcgggcgcgc accgccgcgg agctcgtgac
      841 acccgcacgc tcgagcacct cccacgcgct ctcgagcgcg agccgctgct gcggatccat
      901 cgccgccgcc tcgcgcggag agatcccgaa caacccgggg tcgaaggtct cgaccccggt
      961 gacgaacccg ccctcgcgcg cgtaggtctt gccggccgcg tcgggatcgg ggtcgtagag
     1021 gtccagccct gcccaccgcg ccgggaacgg cccgatcgcg tcgcggccgg cgtcgagcag
     1081 ctgccacagc tcgtccgggg tccggacatg ccccgggagt gcgcacgcca tcgcgctgat
     1141 cgcgatcggc tcgcaccgag cgccttcgag ggcggcgatg cgcgcctcca ggtcggtgtt
     1201 gcgctgcttg agcgcgagca ccgcggccat cgcgcggcgc aatctggtgg cttcggtgtc
     1261 ggacatcgat cgagccttgc tggttcggct gtcgtcagcg gcgcggcgtg cggctcaggc
     1321 ggcccggcgg ccgcacagca gcagcttggg cgacgtcttc tggttcttga gcttgccgac
     1381 gtcggcggtg aacgcgcagc tgtcccagag ctggacatcc tcggcggcgc ggtactgcat
     1441 gatcagcgcg gggcggggcg tgtcgctcca gttgcgatcc gagccgtgga tgatgaagta
     1501 gttgaagaac acgacatcgc cgggctcgcc gtcgatcccg agcgcgcggc tgagcggata
     1561 cctgtccggc ggcagcgcca tgtgcgagaa ccgatcgtgg tgctcgagga gcccgagcct
     1621 gtgcgagccc gggatgatgt ggagtcgccc gttgtgctgc gtcgtcggca cgaggtgcag
     1681 gaatgcggtg cagaagctgt gatcgcggtg gtagaagtac ggcgcgtcct ggtgcatgga
     1741 cttctccatg ccgccgcctg gctccttgta gatcagcatg ccctcgctgt ggatctcgac
     1801 gttcgggccg atcagcgcac cgaggaagtc ggcggtgcgc gggtcgatcg ccgcgcggaa
     1861 cgtcgacgcg gcgtgcatga acgtcggata gaacagctgg atctggtgcg cgcgaccggt
     1921 gctgtcgccg gggcggatca gcgcaccgtg cctggcctcg aaccccgagg cgcggaccgc
     1981 gtcggccatc gcttcggtgt cgcgcaggat cggcgcgacg tgatcgggca gcaggttgcg
     2041 gacgaccacg caaccccacg tatcgtagat cgcgcgcgcc tgcgcgaggg tgcagtccgg
     2101 atagcggatc tcgatgtctt cgatcgtggc ccgcgggatc gcgtcgagat cagcctgctg
     2161 gttgacggcg gcgaggtaac gccgggtgtg ctcgggtgct gacatgggga tggctccagg
     2221 cttcgaggtg ggacgggcgg tcacttgtgc gtggtctcga gatccgcgcg caggtgagcc
     2281 tcggcttcgg cgaaggtcgg cagacccgcg tcgcgcgtgc gctcgtagtg cgtgctgccg
     2341 ttgccgtaga acgcgtggtc gacggtcatc gtggcttgct ggtgggcgtc ggagtagacg
     2401 aagctctcca tcgcctcctc gctggcccac agcgacaggg tcgcggcgcc gccgcagcgc
     2461 tcggaggcgc ccatgcgata gccgagcagg cccggcgtgg cctcggcggc ggcggccgcg
     2521 gtgcgggtcg gggcgtcgag ctcggagagg tattcgagct tggcgaagcc gagcgcgtag
     2581 gccacgatgt agcgctcggg cagctgccca gcgagctcgc cctcgggatc gaacgccggc
     2641 cccttccacg gcgagctgcc gaagtccgac gagtcgcagt ccatgagctc gtgcatcgtg
     2701 cacgtgcggt ggccgcatag ctcccgggtg cggct
//
LOCUS       DASVOR010002399         1519 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_201702,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002399 DASVOR010000000
VERSION     DASVOR010002399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201702"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="VF516_44160"
     CDS             complement(<1..311)
                     /locus_tag="VF516_44160"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEX8114828.1"
                     /translation="MIPLPEIRAAAEHLAGTLHRTPVLSAHSIGARAGVDLRLKCECF
                     QKTGSFKPRGALHKVLSLPAADRARGLVTVSAGNHAQAVAWAARHVAAPCVVVMPTAA
                     PR"
     gene            490..1170
                     /locus_tag="VF516_44165"
     CDS             490..1170
                     /locus_tag="VF516_44165"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEX8114829.1"
                     /translation="MTASPCCVDHIVVTAPDLVVGVKYVSDALGVPMQPGGEHPDMAT
                     HNWLLRLGDAVFLEVIAPNPAAPRPPRRRWFALDDETPATMPRLAGWVARTGDVRAVA
                     AAASEPLGHIEALTRGTLRWLLTVPADGSLPMGGAAPMVLEWQTEGRIAGHIAGHIAS
                     QLDDVGCLLRALHVYHPEAHRLEAVLRSIEFDDPAVVVSPLPAGEPPYLLAEIETPSG
                     PRRLGGRT"
     gene            complement(1193..>1519)
                     /locus_tag="VF516_44170"
     CDS             complement(1193..>1519)
                     /locus_tag="VF516_44170"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8114830.1"
                     /translation="RFTKIGIPYPEFSAPLGGGVEVVGGARVLLGLYTRLAALPLVID
                     MIVAITATKVPILLGYGFWGFAATTGRTGFWPAAHEARLDIMMLSSCLLLLAIGPGKA
                     SFDQRD"
BASE COUNT          208 a          543 c          582 g          186 t
ORIGIN      
        1 cgcggcgcgg cggtcggcat caccaccacg cacggcgccg cgacgtggcg cgccgcccag
       61 gcgaccgcct gtgcgtggtt gccggccgac accgtgacca ggccgcgcgc ccggtcggcg
      121 gccgggagcg acagcacctt gtggagcgcg ccgcgcggct tgaacgagcc ggtcttctgg
      181 aagcactcgc acttgagccg gagatcgacc ccggcgcgcg cgccgatcga gtgtgcggac
      241 agcaccgggg tgcggtgcag cgtgcccgcg aggtgctcgg ccgcggcgcg gatctcgggg
      301 agggggatca tggcgcggga tgataccgcc aagcgccacc taccccgcgc cgcggcggcc
      361 cggatgtgcc gaggacgcgc cgggaatgtg ccggaggcgc ggggacgggg gcatgtgggg
      421 catgcgatgt ccggtcggtc gggatgggcc gggggagccc gggatggcat agatcagaca
      481 ggatcgccga tgactgcctc gccctgctgc gtcgaccaca tcgtcgtgac ggccccggac
      541 ctcgtggtcg gggtgaagta cgtctcggac gcgctcggcg tgccgatgca acccggcggc
      601 gagcatccgg acatggcgac ccacaactgg ctgctccggc tgggcgacgc ggtgttcctc
      661 gaggtcatcg cgccgaaccc ggccgcgccc cggccgccgc ggcgccggtg gttcgcgctc
      721 gacgacgaga cgcctgcgac gatgccccgg ctcgccggct gggtggcgcg caccggcgac
      781 gttcgcgccg tggccgctgc cgcgtccgag ccgctcggcc acatcgaggc gctgacccgc
      841 gggacgctgc gctggctgct caccgtcccc gcggacggta gcctgccgat gggcggcgcg
      901 gcgccgatgg tgctcgagtg gcagaccgag ggccggatcg caggccacat cgcaggccac
      961 atcgcgagcc agctcgacga cgtcggctgc ttgctgcgcg cgctgcacgt ctaccacccg
     1021 gaggcgcacc ggctcgaggc cgtgctgcgc tcgatcgagt tcgatgaccc cgcggtggtc
     1081 gtgtcgccac tgccggccgg cgagccgccc tacctgctcg ccgagatcga gaccccgagc
     1141 gggccgcggc ggctcggcgg gcgaacgtag cggcgctgcc tggcggcgct gcctagtcgc
     1201 gctgatcgaa cgacgccttg ccggggccga tcgcgagcag cagcaagcag ctggacagca
     1261 tcatgatgtc gaggcgcgcc tcgtgggccg ccggccagaa cccggtgcgg ccggtcgtgg
     1321 cggcgaagcc ccagaacccg tagccgagca ggatcggcac cttggtcgcc gtgatcgcaa
     1381 cgatcatgtc gatcaccagc gggagcgcgg ccagccgggt gtacaggccg agcagcacgc
     1441 gcgcgccgcc gacgacctcc acgccgccgc ccagcggcgc ggagaactcg ggatagggaa
     1501 tcccgatctt ggtgaaccg
//
LOCUS       DASVOR010002400         2042 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_172390,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002400 DASVOR010000000
VERSION     DASVOR010002400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172390"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..414
                     /locus_tag="VF516_44175"
     CDS             <1..414
                     /locus_tag="VF516_44175"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX8114831.1"
                     /translation="ITDDQLSQSIVISRTTPGPVGVYVVAVGYMADGLPGAVAGWLAM
                     AAPALTVILLVGTFGKRAQHPRVRSMLQCVVIASAALLVLAAVPIGRDALDSPLMIAI
                     AAVALPVLLSKRIDTLWILGGAAAVSFGAALLGVA"
     gene            514..1482
                     /locus_tag="VF516_44180"
     CDS             514..1482
                     /locus_tag="VF516_44180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114832.1"
                     /translation="MRFMQSPSPCSSLSIARLARTAVVVCGLAALAACAPGIDDSQLA
                     SQAPGSAEVSVRSDEIQSTSAPGFGEVAYHNGRVISTVRVTAVYWNSNVAFQSTLTSF
                     YSTIASSTYIDWLREYNTPTQFIARGSLVNAVVDPSPPANTNLQDTDVTDELVRLINA
                     GTLAVPGPNDLYMVHMPPGFSVTHSGRHSCPGTAFDSYCGYHNRFVYNGTIAYYGIIG
                     DLAPCGSFCGPGTALANTTATASHELLEAITDPGADAWFEDQRLAEISDICQDQDVSV
                     SGFTVMRAWSRLQQDCVVKNPCLSCPSGFSCRCGDFFCRSNTGQCP"
     gene            complement(1550..1726)
                     /locus_tag="VF516_44185"
     CDS             complement(1550..1726)
                     /locus_tag="VF516_44185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114833.1"
                     /translation="MPICRECNDEVTKLVSVKVGGKARKVCADCADRLREEGEVAEAS
                     EGVIQGMMGYKGRR"
     gene            complement(1739..>2042)
                     /locus_tag="VF516_44190"
     CDS             complement(1739..>2042)
                     /locus_tag="VF516_44190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114834.1"
                     /translation="QRAAELDHRAAGAELTAELAHATAVETARCNAVARFRAEAIERL
                     PRVRAMAEASYRSGQGGIVELLDALDAITEARLREIELVAAALDAELDVRAAAHGR"
BASE COUNT          277 a          744 c          688 g          333 t
ORIGIN      
        1 atcaccgacg atcagctcag ccagtcgatc gtgatctcgc ggaccacgcc cggcccggtc
       61 ggggtctacg tcgtcgcggt cggctacatg gccgacggcc tgcccggcgc ggtcgccggc
      121 tggctcgcga tggccgcccc cgcgctcacc gtgatcctgc tggtcggcac gttcggcaag
      181 cgcgcccagc acccgcgggt ccgctcgatg ctgcaatgcg tggtgatcgc cagcgccgcg
      241 ctgctcgtgc tcgccgcggt cccgatcggc cgcgacgcgc tcgactcgcc gctgatgatc
      301 gcgatcgccg ccgtcgccct gcccgtgctg ctctcgaagc ggatcgacac cctgtggatc
      361 ctcggcggcg ccgccgcggt gagcttcggt gcggcgctgc tcggcgtggc ctgaggccac
      421 gggcgccgag ccgaggcgct ctcctgcagt gcagcatctt gctgcggctt cgcccgcgcg
      481 cgagcgccag gggccgcccg atgatgcggc tccatgcggt tcatgcagtc cccatcacct
      541 tgttcatccc tgtccatcgc acggctcgcg cgcaccgcgg tcgtggtctg cgggctcgcg
      601 gcgctcgcgg cgtgcgcccc cggcatcgat gattcccagc tggccagcca ggcgccgggc
      661 agcgccgaag tctcggtccg ctccgacgag atacagagca cgtcggcgcc cgggttcggc
      721 gaggtcgcgt accacaacgg gcgcgtgatc tcgacggtcc gggtcacggc cgtgtactgg
      781 aactcgaacg tggcgttcca gagcacgctc acgagcttct attcgaccat cgccagcagc
      841 acgtacatcg actggctgcg cgaatacaac acacccacgc agttcatcgc gcgcggcagc
      901 ctggtgaatg cggtcgtcga tccgagcccg ccggccaaca ccaacctgca ggataccgac
      961 gtcaccgacg agctggtgcg cctgatcaac gccgggacgc tggcggttcc gggaccgaat
     1021 gacctgtaca tggtccacat gccccccggc ttcagcgtca cccattcggg ccgccattcg
     1081 tgtccgggta cggcgttcga ctcgtattgc ggatatcaca accggttcgt gtacaacggc
     1141 acgatcgcgt attacggaat cattggcgat ctcgcgccgt gcggttcgtt ctgcggcccc
     1201 gggactgcgc tcgccaacac caccgcgacg gcctcgcacg agctgctcga ggcgatcacg
     1261 gatcccgggg cggacgcctg gttcgaggat cagcggctcg ccgagatctc cgacatctgc
     1321 caggaccagg acgtgtcggt ctcgggattc accgtgatgc gggcgtggtc ccgcctgcag
     1381 caggactgcg tggtcaagaa tccatgcctg tcgtgcccgt cgggattctc gtgccgctgc
     1441 ggcgacttct tctgccgatc gaacaccggc cagtgcccgt gagccagatc cggcagtccg
     1501 cgaccggacg ccgagctgcc cgagatcagc gctcgtgcgg gaccgcgcgt cagcgacggc
     1561 ccttgtagcc catcatcccc tggatcacgc cctcgctggc ctcggcgacc tcgccctcct
     1621 cgcggagccg gtcggcgcag tcggcgcaca ccttgcgcgc cttgcctccg accttcacgg
     1681 acacgagctt ggtcacctcg tcattgcact cgcggcagat cggcatgggt ccatggtact
     1741 agcgaccgtg cgcggctgca cgaacatcga gctcggcgtc gagcgcggcg gcgacgagct
     1801 cgatctcacg caggcgggcc tcggtgatgg cgtcgagcgc gtcgagcagc tcgacgatgc
     1861 cgccctgtcc gctgcggtag gacgcctcgg ccatcgcgcg gacgcggggc aggcgctcga
     1921 tcgcctcggc gcggaaccgg gcgaccgcgt tgcaccgggc ggtctcgacc gcggtcgcgt
     1981 gggcgagctc ggcggtgagc tcggcgccgg cggcgcggtg gtcgagctcg gcggcgcgct
     2041 gc
//
LOCUS       DASVOR010002401         1574 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_143086,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002401 DASVOR010000000
VERSION     DASVOR010002401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143086"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1574
                     /locus_tag="VF516_44195"
     CDS             <1..>1574
                     /locus_tag="VF516_44195"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEX8114835.1"
                     /translation="LLRELRAWIDRFGHQLAAARREPELLGPEIRARLRELATRARRL
                     AEAMDFRFLYDEKRRLFHVGYHASADRLDPHHYDLLASEARLASFLAIVWRQVPVEHW
                     FALGRPLGRAGRSGAALRSWGGSMFEYLMPWLLVRSDEDTLLHQSCRAAVERQIAYGQ
                     ERGVPWGISESGYAFVDGQDNYQYRAFGVPGLGLRRGLDEDIVVAPYASLLALPFRAR
                     AVVDNLARLESLGMLGRFGFYEAADFHPARAPGDRPALVFEYMSHHQGMGLVAIANHL
                     ARDAIVERFQADLHVRSGMLLVDERVPAATPAEPAAAEPTESYDVARTPTAALHGWEP
                     DRRTMQVWAIGNDRLTSFVTADGGGGLVWRDLAVTRWEPDPVADRTGIWIYLRDLDTG
                     EVFGATPAPVGGWPADGRVRFEAGSVEFQRRHGELAIRLWIAVGAADDVEVRELEIAN
                     EGDRPRRIEVVGCLEPVLEPRRSADRHPAFSRLFLHASPAPGFAGVVVSRRPRDEAVA
                     PVLLFRMVGERSSAPR"
BASE COUNT          177 a          578 c          589 g          230 t
ORIGIN      
        1 ccctgctgcg cgagctgcgc gcgtggatcg accgcttcgg gcaccagctc gccgcggccc
       61 ggcgcgagcc ggagctgctc ggtcccgaga tccgcgcgcg gctgcgcgag ctcgccaccc
      121 gggcgcgccg gctcgccgag gcgatggact tccgcttcct gtatgacgag aagcggcggc
      181 tgttccacgt cggctaccac gcgtcggcgg atcggctcga tcctcaccac tacgacctgc
      241 tcgcctcgga ggcccgcctg gcgagcttcc tggcgatcgt gtggcggcag gtgccggtgg
      301 agcactggtt cgcgctcggt cgcccgctcg gtcgcgccgg ccggagcggc gcagcgctgc
      361 ggtcgtgggg cggctcgatg ttcgagtacc tgatgccgtg gctgctggtg cgcagcgacg
      421 aggacaccct gctgcaccag agctgtcgtg cggcggtcga gcgccagatc gcgtatggcc
      481 aggagcgcgg cgtgccgtgg gggatctcgg agagcggcta cgcgttcgtc gacggccagg
      541 acaactacca gtaccgcgcg ttcggcgtcc ccgggctcgg gctccggcgc ggcctcgacg
      601 aggacatcgt cgtcgcgccc tacgcctcgc tgctcgcgct gccgttccgc gcccgcgcgg
      661 tggtcgacaa cctggcgcgc ctcgaatcgc tcggcatgct gggtcgcttc gggttctacg
      721 aggcggccga cttccatccc gcgcgcgccc cgggagaccg cccggcgctg gtgttcgagt
      781 acatgtccca ccaccagggc atgggcctgg tcgcgatcgc caaccacctg gcgcgcgatg
      841 cgatcgtcga gcggttccag gccgaccttc acgtccgcag cggcatgctg ctcgtcgacg
      901 agcgcgtgcc cgccgcgaca ccggccgagc ccgcggcggc cgagccgacc gagagctacg
      961 acgtcgcgcg aacgccgacc gcagcgctcc acggctggga gcccgaccgg cgcaccatgc
     1021 aggtctgggc gatcggcaat gaccggctga cctcgttcgt caccgccgac gggggcggcg
     1081 ggctggtgtg gcgcgatctc gcggtgaccc gctgggagcc cgatccggtc gccgatcgca
     1141 ccgggatctg gatctacctc cgcgacctcg acaccggcga ggtgttcggc gcgacgccgg
     1201 cgccggtcgg cggctggccg gccgatggcc gcgtccggtt cgaggccggg tcggtcgagt
     1261 tccagcgccg ccacggcgag ctggcgatcc ggctgtggat cgcggtcggc gccgccgacg
     1321 acgtggaggt ccgcgagctg gagatcgcca acgagggcga ccggccgcgc cgcatcgagg
     1381 tcgtgggttg tctcgagccg gtgctcgaac cgcggcgcag cgcggaccgc cacccggcgt
     1441 tctcccggct gttcctgcac gcgtcaccgg cgcccggctt cgccggcgtc gtggtgtcgc
     1501 gccgcccgcg cgacgaggcc gtggcgccgg tgctgctgtt ccgcatggtc ggcgagcggt
     1561 cgagcgctcc gcgc
//
LOCUS       DASVOR010002402         3961 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_115118,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002402 DASVOR010000000
VERSION     DASVOR010002402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3961)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3961)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3961
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115118"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3692
                     /locus_tag="VF516_44200"
     CDS             <1..3692
                     /locus_tag="VF516_44200"
                     /inference="COORDINATES: protein motif:HMM:NF013309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ADP-ribosyltransferase"
                     /protein_id="HEX8114836.1"
                     /translation="AGPALTAMPHAAARQIQRFSLGDLNPLNALKDAAKAILEKLTGS
                     ADDGKAQLQSEGTSGTTEINTAAQTKGAEVDGQGQQKGGEVDGQGQQKGGEVDGQGQQ
                     KGGEIDGQGQDKGGEIDGQGQQKGGEVDAAGQQKGGEVDAAGQQKGGEVDAAAHVHAA
                     ALETEATAHGNQLETEGAARHRELETEGNTKQHELDTAAADKHRELDADAHSKGAELH
                     GEAAAHRAELSADYGRLDASAQADASQVHATVAADTDAVQAEAQSSSNVVSSAWHGLE
                     SEAMSLAQGLDREAGELCDRFQGKAKDYIKKIDSIVQPVFDKIGDGFKAIQDEAEKAW
                     HAFLQKIAPILKTLEDKWNQFTAWARKVWDPIEKKLVEIGKKALELAEKAWHKAQQGW
                     EWLKTKASQIWNTLKQKLTAAVQWLASRGDAARAWIHGKAEAARQWIHGKAAAARQWI
                     HGKAEAAQQWVHGTADAARAWIHGKADAARAWIHDKAGAARSWIHDKADAARNWMHDK
                     ADAAGKWIADKGHALVNGLSSKAKGLVDHITSGGGPILRFFASAVDGLIDGVSSVGNA
                     AVDGLSSAVDAGAHFVADKASAAVSWVESKATGAVSWVESKATGAVTWVENKATGAVN
                     LVEAKASGAISWVESKAIGAVNWVENKATGAVDLLERGATMAVNGLERVATGAVNLVE
                     TAIKLGLGALALIGKGALELVQGAWDGIKQAWGFLSDKLGQAWNWVKDKASQAWDWVN
                     QNVIQPSWQWLQKQWGDLKAWFTKTFPGLTKCWEVFKEYAGAVGGYLLQKLAQYAAWF
                     KSLPAWMQLLIFPVVPLGEWMMLMNAWDAVPEPIRTAIHLLLDVLTFIPEIGAIPAAI
                     NSVLYLLEGDILNGTLSALVALAMLVPGLGDAAESANVLAKLAEAVGGADKLAMLIKE
                     LGSVEKVAELIKDVGSLEKLGALIKEVGGVEKLIELMHEVGGAGKLVELIKEFKDVET
                     LGKLIKELGSADEVLKLANDAGGAERLINLMKLAGGDVAGLKKALDFYKDGADLERLL
                     QNGRTLAELNKLADSSTAFQALLGGKYASLMADARALRADPAIARLIAGLTDEEVAAM
                     IGYTSSDYRFLNAALRSGDPAQLLKAAPYIDRAVAGLDALPSYTGLVYRGVESAGLPA
                     AVKAQYVVGGVIEESAFTSSSYVAGANFSGDWQFTILSKTGKQIDFLSQFGTEKEVLF
                     RPGTRFKILDIVKSGGKTLIRMMEL"
     gene            3692..>3961
                     /locus_tag="VF516_44205"
     CDS             3692..>3961
                     /locus_tag="VF516_44205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114837.1"
                     /translation="MPELTDEEKRILELMRQLEASDPVVPRAARNQDFLPGVDDQGNP
                     IPVSGPLPSAEQQAADARRLIRELEAQVAARDAAGKPPTDPAAPGT"
BASE COUNT          707 a         1316 c         1478 g          460 t
ORIGIN      
        1 ccgcgggtcc ggcgctgacc gcgatgccgc acgccgccgc gcgccagatc cagcggttct
       61 cgctcggcga tctcaacccg ctcaacgcgc tcaaggacgc ggcgaaggcg atcctcgaga
      121 agctgaccgg ctccgccgac gacggcaagg cccagctgca gagcgagggc acgtcgggga
      181 ccaccgagat caacaccgcg gcccagacca agggcgccga ggtcgacggc cagggccagc
      241 agaagggcgg cgaggtcgac ggccagggcc agcagaaggg cggcgaggtc gacggccagg
      301 gccagcagaa gggtggcgag atcgacggcc agggccagga caagggcggc gagatcgacg
      361 gccagggcca gcagaagggc ggcgaggtcg acgccgcggg ccagcagaag ggcggcgagg
      421 tcgacgccgc aggccagcag aagggcggcg aggtcgacgc cgccgcccac gtccacgcgg
      481 cggcgctcga gaccgaggcc accgcgcacg gcaaccagct cgagaccgag ggcgcggcgc
      541 gccaccgcga gctcgagacc gaggggaaca ccaagcagca cgagctggac accgcggcgg
      601 ccgacaagca ccgcgagctc gacgccgacg ctcacagcaa gggcgccgag ctgcacggcg
      661 aggccgccgc ccaccgcgcc gagctgtcgg ccgactacgg taggctcgac gccagcgccc
      721 aggccgacgc cagccaggtc cacgcgacgg tcgccgccga taccgacgcg gtccaggccg
      781 aggcccagag ctcgagcaac gtcgtctcgt ccgcctggca cggcctcgag tccgaggcga
      841 tgtcgctcgc ccagggcctc gatcgcgagg ccggcgagct gtgcgatcgg ttccagggca
      901 aggccaagga ctacatcaag aagatcgact cgatcgtcca gccggtgttc gacaagatcg
      961 gcgacggctt caaggcgatc caggacgagg ccgagaaggc ctggcacgcg ttcctccaga
     1021 agatcgcgcc gatcctcaag acgctcgagg acaagtggaa ccagttcacg gcgtgggcgc
     1081 gcaaggtctg ggacccgatc gagaagaagc tggtcgagat cggcaagaag gcgctggagc
     1141 tcgccgagaa ggcctggcac aaggcccagc agggctggga gtggctcaag accaaggcct
     1201 cccagatctg gaacacgctc aagcagaagc tgaccgcggc ggtgcagtgg ctcgcgagcc
     1261 gcggcgacgc cgcgcgcgcg tggatccacg gcaaggccga ggcggcgcgg cagtggatcc
     1321 acggcaaggc ggccgcggcg cggcagtgga tccacggcaa ggccgaggcg gcgcagcagt
     1381 gggtgcacgg caccgcggat gcggcgcgcg cgtggatcca cggcaaggcc gacgcggcgc
     1441 gcgcgtggat ccacgacaag gcgggcgcgg cgcggagctg gatccacgac aaggcggacg
     1501 cggcgcgcaa ctggatgcac gacaaggccg acgccgccgg caagtggatc gccgacaagg
     1561 gccacgcgct ggtcaacggg ctgtcgagca aggccaaggg cctggtcgat cacatcacca
     1621 gcggcggcgg tccgatcctg cggttcttcg ccagcgcggt cgacggcctg atcgacggcg
     1681 tgtcgagcgt cggcaacgcc gcggtcgacg ggctgtcgtc ggcggtcgac gccggtgcgc
     1741 acttcgtcgc cgacaaggcg agcgccgcgg tcagctgggt cgagagcaag gcgaccggcg
     1801 cggtcagctg ggtcgagagc aaggcgaccg gcgcggtgac ctgggtcgag aacaaggcga
     1861 ccggcgcggt caacttggtc gaggccaagg cgagcggcgc gatcagctgg gtcgagagca
     1921 aggcgatcgg cgcggtcaac tgggtcgaga acaaggcgac cggcgcggtc gacctgctcg
     1981 agcgcggtgc gacgatggcg gtcaacggcc tcgagcgggt cgcgaccggc gcggtcaacc
     2041 tggtcgagac cgcgatcaag ctcgggctcg gcgcgctcgc gctgatcggc aagggcgcgc
     2101 tcgagctcgt gcagggcgcg tgggacggga tcaagcaggc gtggggcttc ctcagcgaca
     2161 agctcggcca ggcgtggaac tgggtcaagg acaaggccag ccaggcgtgg gactgggtca
     2221 accagaacgt gatccagccg tcgtggcagt ggctgcagaa gcagtggggc gatctcaagg
     2281 cctggttcac gaagacgttc ccgggcctca ccaagtgctg ggaggtgttc aaggagtacg
     2341 ccggcgcggt cggcggctac ctgctgcaga agctggcgca gtacgccgcg tggttcaagt
     2401 cgctaccggc gtggatgcag ctcttgatct tcccggtcgt cccgctcggc gagtggatga
     2461 tgctgatgaa cgcgtgggac gcggtgcccg agccgatccg caccgcgatc cacctcttgc
     2521 tcgacgtgct gaccttcatc ccggagatcg gcgcgatccc ggccgcgatc aactcggtgc
     2581 tgtacctgct ggagggcgac atcctcaacg gcacgctgtc ggcgctggtc gcgctcgcga
     2641 tgctggtccc cggcctgggc gacgcggccg agagcgcgaa cgtgctcgcc aagctcgccg
     2701 aggcggtcgg cggcgccgac aagctggcga tgctgatcaa ggagctcggc agcgtcgaga
     2761 aggtcgccga gctgatcaag gacgtcggca gcctggagaa gctcggcgcg ctgatcaagg
     2821 aggtcggcgg cgtcgagaag ctgatcgagc tgatgcacga ggtcggcggc gccggcaagc
     2881 tggtcgagct gatcaaggag ttcaaggacg tcgagaccct cggcaagctg atcaaggagc
     2941 tgggcagcgc cgacgaggtc ctcaagctcg cgaacgatgc cggcggcgcc gagcgcctga
     3001 tcaacctgat gaagctcgcc ggcggcgacg tcgcggggct caagaaggcg ctcgacttct
     3061 acaaggacgg cgccgacctc gagcgcctgc tgcagaacgg ccgcacgctg gccgagctca
     3121 acaagctcgc cgactcgagc accgcgttcc aggcgctgct cgggggcaag tatgcgagcc
     3181 tcatggccga cgcccgcgcg ctgcgcgccg atccggcgat cgcccggctg atcgccggcc
     3241 tgaccgacga ggaggtcgcg gcgatgatcg gctacacctc gtcggactac cgcttcctca
     3301 acgccgcgct gcggtccggc gacccggccc agctgctcaa ggccgcgccg tacatcgacc
     3361 gcgcggtcgc ggggctcgat gcgctgccca gctacaccgg cctggtctac cgcggcgtcg
     3421 agagcgccgg cctgcccgcg gcggtcaagg cccagtacgt ggtcggcggg gtgatcgagg
     3481 agtcggcgtt caccagcagc tcgtacgtcg cgggcgccaa cttctccggc gactggcagt
     3541 tcacgatcct gtccaagacc ggcaagcaga tcgacttcct gtcccagttc ggcaccgaga
     3601 aggaggtcct gttccggccc ggcacgcggt tcaagatcct cgacatcgtc aagtcgggtg
     3661 gtaagacgct catcaggatg atggagctgt gatgccggag ctcaccgacg aggagaagcg
     3721 gatcctggag ctgatgcgcc agctggaggc cagcgatccg gtggtgccgc gcgcggcccg
     3781 caaccaggac ttcctgccgg gcgtcgacga tcagggcaac ccgatcccgg tgagcgggcc
     3841 gctgccgtcg gccgagcagc aggcggccga cgcgcgccgg ttgatccgcg agctcgaggc
     3901 ccaggtcgcc gcccgcgacg ccgccggcaa gcccccgacg gaccccgccg cgccgggcac
     3961 g
//
LOCUS       DASVOR010002403         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_377050,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002403 DASVOR010000000
VERSION     DASVOR010002403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377050"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..696)
                     /locus_tag="VF516_44210"
     CDS             complement(<1..696)
                     /locus_tag="VF516_44210"
                     /inference="COORDINATES: protein motif:HMM:NF039450.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADYC domain-containing protein"
                     /protein_id="HEX8114838.1"
                     /translation="MTRLCMTGIAALSVSACASDVVAMSSSEQAATVLQPMMQQGTEL
                     QGMQLQGMQLQGMQLQGASLGGGPLSNLRVENGELVAEQDQITLRGAALVGVHLYAQA
                     ADAAADPPVTVSVEYRITDIAEEDPRYDPTDTGSTFLYTLEQNVDHTGVWQPACRPDL
                     DGRSVAIPVAATWNAQGDRVESSSLFTLGCTTGVIAKCYRWGYRPWVTGYGDLVAMHW
                     ACTRLARADYCGDG"
     gene            complement(769..1563)
                     /locus_tag="VF516_44215"
     CDS             complement(769..1563)
                     /locus_tag="VF516_44215"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HEX8114839.1"
                     /translation="MPPDRFLFGSADGAELRLGFLNAAPLHEVQTAGFFIGRYEVTFA
                     EWIEFLDDLPPAERSRRTPRAAIVQLSLALTEIEPRRWQLGLTSSGRTYTAQTGQRLH
                     YEGRERRADQDWTRFPVSAVSYDDAIAYAVWLDRTGRLPGARLCDEYEWERAARGADA
                     RTFPGGPALAPDDANLDATYGRQPGAFGPDEVGSHPGSRSPVGADDMAGNAWEMTRSV
                     QTPGTPVFRGGSWYTGALSARSANREPGEPTERDVRIGLRLCATPQ"
     gene            complement(1615..>2107)
                     /locus_tag="VF516_44220"
     CDS             complement(1615..>2107)
                     /locus_tag="VF516_44220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114840.1"
                     /translation="GALALYDADRWGEGEIAWTGVAALAAREEHQYLVASGNLETALR
                     LDPARVDLRSDFADLTFERLLRAERDRHPDLVDELAVRLAAYDDGHRQAALAADGRIE
                     LEVEPPGTTVWSERPGGARELAGQAPLSARTLPPGSLILSFEAPGRVPARLPVLVPRG
                     QTL"
BASE COUNT          296 a          760 c          737 g          314 t
ORIGIN      
        1 gccgtcgccg cagtagtcgg cgcgcgcgag ccgcgtgcag gcccagtgca tcgcgaccag
       61 gtcgccgtag cccgtgaccc acggtcgata accccagcgg tagcacttcg cgatcacgcc
      121 ggtggtgcag ccgagggtga acagcgagct cgattcgacg cgatcgccct gcgcgttcca
      181 cgtggcggcg accgggatcg cgacgctgcg gccgtcgagg tccggccggc aggcgggctg
      241 ccagacgccg gtatggtcga cattctgctc gagcgtgtag aggaaggtcg atccggtgtc
      301 cgtcggatcg tacctgggat cttcctccgc gatatcggtg atccggtact cgacggacac
      361 ggtcaccggc ggatcggctg ccgcgtccgc ggcctgggcg tacaggtgca cgccgaccag
      421 cgcggcgccg cgcagcgtga tctgatcctg ctcggccacc agctcgccgt tctcgacccg
      481 caggttgctc agcgggccgc ctcccaggct cgccccctgc agctgcatgc cctgcagctg
      541 catgccctgc agctgcatgc cctgcagctc cgtgccctgc tgcatcatgg gctgcagcac
      601 cgtggcagct tgctcgctgc tggacatcgc gacgacgtcg gatgcacaag cagatacgct
      661 cagcgccgcg atcccggtca tacagagtct tgtcacgatc gaagtctctt ggtccatctg
      721 gattgtcccc ttttgtgaga tccagtgaga tccggtgtat gcaatcgatc actgtggtgt
      781 ggcacacagc cgcaggccga tgcgcacgtc gcgctcggtg ggctcgccgg gttcgcggtt
      841 cgcgctgcgc gccgacagcg ccccggtgta ccagctgcca ccgcgaaaca cgggcgtacc
      901 cggtgtctgc accgagcgcg tcatctccca ggcgttgccg gccatgtcgt ccgcgccgac
      961 cgggctgcgc gagcccggat gcgagcccac ctcgtcgggg ccgaacgcgc cgggctgccg
     1021 accgtacgtc gcgtcgaggt tcgcatcgtc gggcgccagc gcgggcccgc cgggaaacgt
     1081 ccgcgcatcc gccccgcggg cggcgcgctc ccactcgtac tcgtcacaca gccgcgcacc
     1141 cgggagccgc ccggtgcgat cgagccagac agcgtaggcg atcgcatcgt cgtacgacac
     1201 cgcggagacc gggaacctcg tccagtcctg atcggctcgc cgctctcttc cctcgtagtg
     1261 cagccgctgc ccggtctgtg ccgtgtaggt ccgccccgac gacgtcaagc cgagctgcca
     1321 ccgcctgggc tcgatctcgg tgagcgcgag cgagagctgc acgatggccg cgcgcggggt
     1381 gcgacggctg cgctcggcgg gcgggagatc gtcgaggaac tcgatccatt cggcgaaggt
     1441 gacctcgtac cggccgatga agaagccggc ggtctggacc tcgtggagcg gtgccgcgtt
     1501 gagaaacccc agccgcagct cggcgccatc cgcgctgccg aacaggaacc ggtccggggg
     1561 cacgtagatc atcccgcggg gcgccgacgc cgacgccggg agcgcgaccg cgatctacag
     1621 cgtctgtccg cgcggcacca ggacggggag gcgcgccggc acgcgcccgg gcgcttcgaa
     1681 tgacaggatc agcgagccgg gcggcagcgt ccgtgccgac agcggcgcct ggcccgcgag
     1741 ctcgcgcgcc ccccccgggc gttcactcca cacggtcgtg cccggcggct cgacctcgag
     1801 ctcgatgcgc ccatcggcgg cgagcgctgc ctggcggtgg ccgtcgtcgt acgcggccag
     1861 ccgcacggcg agctcgtcga cgaggtcggg gtggcgatcg cgttcggcgc gcagtagccg
     1921 ctcgaacgtg aggtcggcaa agtcgctgcg tagatcgacg cgcgcgggat cgagccgcaa
     1981 ggcggtctcg aggttgccgg acgcgacgag gtactggtgc tcctcgcgcg ccgccagcgc
     2041 cgcgactccg gtccaggcga tctcgccttc gccccagcga tcggcatcgt acagcgcgag
     2101 cgctccc
//
LOCUS       DASVOR010002404         4942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_347121,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002404 DASVOR010000000
VERSION     DASVOR010002404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347121"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            411..1793
                     /gene="murG"
                     /locus_tag="VF516_44225"
     CDS             411..1793
                     /gene="murG"
                     /locus_tag="VF516_44225"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEX8114841.1"
                     /translation="MRLLIAGGGTGGHLFPGVAIAEELRAREPGAAVRFVGTRRGIEA
                     RVLPELGWELSLIEVSGLKTVGALGALRGLLRLPRALWQARRIVREFRPDAVIGVGGY
                     ASGPVVLMARLAGVPTAICEQNSIPGLTNKILGRIVRAVFVSFEGTRRFFQPAKTVVS
                     GNPVRRGLVQKLLEARPAAPAAASAPPARPAAPSAASPHDSAESSHPSPAASPSASPL
                     AISPDDSAESRPGPMTTSPTAAPNTSAESSPTAAPNASAESSPMPGSMMASAAGEART
                     MVHVLVSGGSLGAVAVNPLAADALIALAHEAPLAIVHQTGDKGLTETVDRYRAAGVAA
                     ECHAFIKDMAAAYQRADIILCRAGATTVAELAITGKPAVFIPYPFAADNHQELNARAM
                     ADAGAALMFRQSELTADKLAGALRPLVTDPARRAEMGAKMKALARPAAAAAVIDWCES
                     QRPAPGAPSH"
     gene            2347..3792
                     /gene="murC"
                     /locus_tag="VF516_44230"
     CDS             2347..3792
                     /gene="murC"
                     /locus_tag="VF516_44230"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEX8114842.1"
                     /translation="MFRKQDTKIHFVGIGGMGMCGIAEVLANMGYRVSGSDMNDTEIT
                     RHLAKIGCTVKQGHRADNLGEADVVVVSSAIRGYNPEVEAARARQIPVIPRAEMLGEL
                     MRMKYGIAIAGAHGKTTTTTMVHTVLMAAGLDPTAVIGGRVNSLGLANARWGKSDYLV
                     AEADESDGSFLTLTPTVAVVTNVDAEHLDHYGTFDSVKKAFTDFCNRVPFFGMSALCL
                     DDAGVQAILPNLTKRYTTFGIASQATYRARHIRHDGLITRFVAWRRADELGEVALPMP
                     GTHNVLNALGVLAIADFLEIPFATCAQALAQFEGVARRFTVRGEVGGVTVVDDFGHHP
                     AEVRATLAGARAAFAGRRVVAAFQPHRYTRTRDQLGEFARAFHDADQVAICDIFAAGE
                     RPIDGVSSEALVRLAREAGHSGVAYIPRREDLAAWLDAQAKPGDLVITLGAGNIQLVC
                     NEVIALLEQRLGAATRKNLVRIDESSATVAR"
     gene            complement(3803..4513)
                     /locus_tag="VF516_44235"
     CDS             complement(3803..4513)
                     /locus_tag="VF516_44235"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEX8114843.1"
                     /translation="MYSESAALYDLIYTPIKDYSAEAGQVAGLLRRARPRSRTLLDVA
                     CGTAEHARLLGEVHGFEVDGVDLDPSFVAIARAKCPQGRFDVADMADFHLGRSYDVVL
                     SLFGSIGYVVTLPRLGEALACLHDHLVPGGVAIVEPFLTPDAFRPGASGAHTVESGDL
                     RITRTHRSERDGQRCRLYFHDTIEGPDGTRQSTEIHELGLFTVDEMLAAFSAVGLAVT
                     YDPNSSIGRGVYVASALA"
     gene            4621..>4942
                     /locus_tag="VF516_44240"
     CDS             4621..>4942
                     /locus_tag="VF516_44240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114844.1"
                     /translation="MSAVEVWQPRADAWRRVHEARTSPRQAVGPSEISLEFSGDGARC
                     RKLMETAAEIYRGPVVSPVAGSEVELIGDEELGDDDASAVDNPRSRVDPAARRALREL
                     LGDGV"
BASE COUNT          724 a         1726 c         1819 g          673 t
ORIGIN      
        1 ctccgtcgtc gccggtggga ttttcacccc gggtcgctct ccgaaatttc gggctgctac
       61 atgccttcct ttccggccag gcttgtcctg gcgccctcgc ggcgtccgac cccatcctgc
      121 tcgcggtcgc ccccggcgag cgagcgttgg cgtcccgcga tcgcgccacg aggcgcccac
      181 cacgtggcgc gaagccgtct tcctccagcg ccgtcctccc gccagcccga cgagctacgt
      241 gctcctggcc cggccagcgg gaatcggctc cgccgctcgg acgttttgcg tccatgacga
      301 gatttgccta tgcaggaccg cacccggagg ctgcgtacgc gcggagaccg acgctcgaag
      361 ccgaccaggg accgcaaccg cgggctgcgt cctgtggtag tagcgtggcg atgcggctcc
      421 tgatcgccgg cggtggcacc ggcgggcacc tgtttcccgg cgtggcgatc gccgaggagc
      481 tgcgcgcccg cgagcccggt gccgcggtgc gcttcgtcgg cacccggcgc ggcatcgagg
      541 cccgtgtgct gcccgagctg ggctgggagc tgtcgctgat cgaggtgtcg gggctcaaga
      601 cggtgggcgc gctgggcgcg ctgcgcgggc tgctccggct gccgcgcgcg ctgtggcagg
      661 cgcggcggat cgtccgcgag ttccggccgg atgccgtgat cggggtcggc ggctacgcgt
      721 cgggcccggt ggtgctgatg gcgcggctcg ccggcgtgcc gacggcgatc tgcgagcaga
      781 actcgatccc cgggctcacc aacaagatcc tcggtcgcat cgtgcgcgcg gtgttcgtgt
      841 cgttcgaagg cacccgccgg ttcttccagc cggccaagac ggtggtcagc ggcaacccgg
      901 tgcgccgcgg tttggtgcag aagctgctcg aagcccgccc ggcagcgccg gccgcggcat
      961 cggcccctcc agcgaggccg gcggcgccat ccgccgcatc gccgcatgat tccgcggaat
     1021 cgtcccatcc ctctcccgcc gcatcgccca gcgcttcacc gctcgccatc tcgccagacg
     1081 attccgcgga gtcgcgccct ggtcccatga ccacttctcc caccgccgca ccaaacacct
     1141 ccgcggaatc gtcccccacc gccgcaccaa acgcttccgc ggaatcgtcc cccatgcctg
     1201 gcagcatgat ggcgagcgca gctggggagg cgcgcacgat ggtgcacgtc ctggtcagcg
     1261 gcggctcgct cggcgcggtc gcggtcaacc cgctggccgc cgacgcgctg atcgcgctcg
     1321 cgcacgaggc gccgctggcg atcgtccacc agaccggcga caaggggctg accgagaccg
     1381 tcgaccgcta ccgcgcggcg ggcgtcgccg cggagtgcca cgcgttcatc aaggacatgg
     1441 cggcggccta ccagcgcgcg gacatcatcc tctgccgcgc cggcgccacc acggtcgccg
     1501 agctggcgat caccggcaag cccgccgtgt tcatcccgta cccgttcgcc gccgacaacc
     1561 accaggagct caacgcccgc gcgatggccg acgccggggc agcgctgatg ttccggcagt
     1621 ccgagctgac ggcggacaag ctggccggcg cgctacggcc gctggtgacc gacccggcgc
     1681 gccgcgccga gatgggcgcg aagatgaagg cgctggcccg ccccgcagca gccgcggcgg
     1741 tcatcgactg gtgcgagtca cagcggccag cgccaggcgc tccctctcac tgaccgtcgt
     1801 gcagtgccgg ctcggtgcgc cgaatagcgg ggaagctgtc ggaaccccgc gcgggcagcc
     1861 gtaggacggc aacgcgagcg cgaggagccg cgggggttgc tccgagcggt agcttccacg
     1921 gccggtgaca ggaggttgag tactgtcaga ggtccttcga cgagccatcg cgagacgtca
     1981 acgcgaccgc ggggggcgac cgcgagcagg atggggtcgt ggacgcgacc gtgatctgga
     2041 taggccgttc gttgccccgc acatacctag aggggttcgc acaggacggg tggtgattcg
     2101 gcgggcgcgg ccccgacccc atccgcgcag cagcccccgg cgagtcgcgt gtgcgcactc
     2161 tcctgggcga ccgcgccgtg agcgagcgcg tccgcggtct gcacagacag gtgcgagggc
     2221 acggcgtgcg cggagagcag agcgcggggc acggcgaccg ggtgatcgca tcgcgatagt
     2281 ctgctagaaa cgccggcggc ccggcatgct cgggccggct gcagcgttga tccacacgag
     2341 gccccgatgt ttcgcaagca ggacaccaag attcacttcg tcggcatcgg cgggatgggg
     2401 atgtgcggga tcgccgaggt gctcgccaac atgggctacc gggtctcggg ctcggacatg
     2461 aacgacaccg agatcacccg gcacctggcg aagatcggct gcacggtgaa gcagggccac
     2521 cgcgcggaca acctggggga ggccgacgtg gtggtcgtgt cgagcgcgat ccggggctac
     2581 aaccccgagg tcgaggccgc gcgggcgcgc cagatcccgg tgatcccgcg cgccgagatg
     2641 ctcggcgagc tgatgcggat gaagtacggg atcgcgatcg ccggggcgca cggcaagacc
     2701 acgacgacga cgatggtgca cacggtgctg atggcggccg ggctcgaccc gacggcggtg
     2761 atcggcgggc gggtcaactc gctcggcctg gccaacgcgc ggtggggcaa gagcgactac
     2821 ctggtcgccg aggccgacga gagcgacggc tcgttcttga ccctgacgcc gacggtcgcg
     2881 gtggtgacca acgtcgatgc cgagcacctg gatcactacg gcacgttcga cagcgtcaag
     2941 aaggcgttca ccgacttctg caaccgggtg ccgttcttcg ggatgagcgc gctgtgcctc
     3001 gacgacgccg gggtgcaggc gatcctgccc aacctgacca agcgctacac gacgttcggg
     3061 atcgcgtcgc aggcgacgta ccgcgcgcgc cacatccggc acgacggcct gatcacgcgg
     3121 ttcgtggcct ggcgccgggc cgacgagctc ggcgaggtcg cgctgccgat gccggggacg
     3181 cacaacgtgc tgaacgcgct cggggtgctc gcgatcgccg acttcctgga gatcccgttc
     3241 gcgacctgcg cgcaagcgct ggcgcagttc gagggcgtgg cgcggcggtt cacggtgcgc
     3301 ggcgaggtcg gcggggtcac cgtggtcgac gacttcggcc accacccggc ggaggtccgc
     3361 gccacgctgg ccggcgcccg ggcggcgttc gccggccgcc gcgtggtcgc cgcgttccag
     3421 ccccaccgct acacgcggac gcgcgatcag ctcggcgagt tcgcgcgggc attccacgac
     3481 gccgatcagg tggcgatctg cgacatcttc gccgccggcg agcggccgat cgacggcgtg
     3541 tcgagcgagg cgctggtccg gctcgcgcgc gaggccggcc actccggggt cgcgtacatc
     3601 ccgcggcgcg aggacctggc ggcgtggctc gacgcccagg ccaagccggg cgacctggtg
     3661 atcacgctcg gggccggcaa catccagctg gtgtgcaacg aggtgatcgc gctgctcgag
     3721 cagcggctcg gcgcggcgac ccgcaagaac ctggtccgga tcgacgagtc gagcgcgacg
     3781 gtggcgcgct aggtgaggca gcctacgcca gcgccgacgc gacgtagacg ccgcggccga
     3841 tcgagctgtt cgggtcgtag gtcacggcga gcccgacggc ggagaacgcg gcgagcatct
     3901 cgtcgacggt gaacagcccg agctcgtgga tctcggtcga ctgccgcgtc ccgtcggggc
     3961 cctcgatcgt gtcgtggaag tacagccggc agcgctggcc gtcgcgctcg gagcggtggg
     4021 tgcgcgtgat gcgcaggtcg ccggactcga cggtgtgcgc gccgctcgcg ccggggcgga
     4081 acgcgtcggg agtgaggaac ggctcgacga tggcgacgcc gccgggcacg aggtgatcgt
     4141 gcaggcaggc cagcgcctcg cccagccgcg gcagcgtgac cacgtagccg atcgagccga
     4201 acaggctgag cacgacgtcg taggaccggc cgaggtggaa gtcggccatg tcggcgacgt
     4261 cgaaccggcc ctgcgggcac ttcgcgcggg cgatcgcgac gaacgacgga tcgagatcga
     4321 cgccgtcgac ctcgaagccg tggacctcgc cgagcaggcg cgcgtgctct gccgtaccgc
     4381 acgcgacgtc gagcagcgtg cgggagcgcg ggcgcgcccg gcgaagcagg ccggcgacct
     4441 gcccggcttc ggcggagtaa tccttgatgg gcgtgtagat cagatcgtac agcgcagccg
     4501 actcggaata catgccggtc ctcccccgct cgccgcacgt gaggcgcagc gaacgacgac
     4561 acctgagcac gaactcctgt gacaggcacg cacttcgtga cgccacgtat gggcgtgcgg
     4621 atgagcgcag tcgaggtgtg gcagccgcgc gctgacgcgt ggcgacgagt gcacgaagcg
     4681 cgcacgagcc cgcgccaagc ggttggtccc tcggagatct ccctggaatt ttccggcgac
     4741 ggcgcgcgtt gcagaaagtt gatggagacc gccgcggaga tctatcgtgg tccggtggtt
     4801 agcccagtcg ccggcagcga agtcgagctg atcggcgacg aggagctcgg cgacgacgac
     4861 gccagcgcag tggacaatcc gcgctcgcgg gtcgaccccg ccgcccgccg cgcgctgcgc
     4921 gagctgctcg gcgacggcgt gc
//
LOCUS       DASVOR010002405         1522 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_172425,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002405 DASVOR010000000
VERSION     DASVOR010002405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172425"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..>1522
                     /locus_tag="VF516_44245"
     CDS             172..>1522
                     /locus_tag="VF516_44245"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8114845.1"
                     /translation="MAIDLETLDAALAALEDFARRNLPDAKLRELDRDDEFPEALVRR
                     MFSDELGVQLFFIPEKYGGLGAGAFGVYRICEQLAAVDVGIATGLLATFLGSEPISVG
                     GTEDQQRRWLGQIAEHGILMAYGATEPAAGSDLAALRTVAERVEDGGKRVGYRITGSK
                     QWISNGGVADLYTILANAPGGPTWFVVDKGTPGLTPGKPEDKHGIRASNTAALALDQV
                     YVDADRVVGGAEGQGLVQAQAVFGYTRLMVAAFGLGAGWAALNRAIEYSTTRIQAGTP
                     LAQKQGYTHKLIVPHAARLEAARAYIEEVGVRLDAGEDPLHTEGAIAKYLATEAGNAA
                     ADASIQALGGYGYTREYMVEKVKRDVRITTIYEGTSEIMEMTIARDRWQTHLKTRGQH
                     YHEAAAQLEALHRRRPEVGANIAALAAHALSDVLERARLARLTRHQHILLRLGELIAW
                     "
BASE COUNT          237 a          538 c          565 g          182 t
ORIGIN      
        1 cgagctgctc aagcagggca aggtcgatct cgcgcaggcc gcggctcggg atctcacgga
       61 aggggcgcac ggcaagcctt gcgcggagcc ggcggccggc agtgcgggca cgcaaggcat
      121 gcgcagaacc cggcggctgc agcggcccga accgtgcttg ttcccgggcg catggcgatc
      181 gacctggaga ccctggacgc ggcgctggcg gcgctggagg acttcgcacg gcgcaacctt
      241 ccggatgcga agctccgcga gctcgaccgc gacgacgagt ttcccgaggc gctggtgcgc
      301 cggatgttca gcgacgagct cggcgtccag ctgttcttca tccccgagaa gtacggcggg
      361 ctgggcgccg gggcgttcgg cgtctaccgg atctgcgagc agctggcggc ggtcgacgtc
      421 gggatcgcga ccggcctgct cgcgacgttc ctcggcagcg aaccgatcag cgtcggcggg
      481 accgaggacc agcagcggcg gtggctgggg cagatcgccg agcacggcat cctcatggcg
      541 tacggcgcca ccgagccggc cgcgggcagc gacctcgccg cgctgcgcac cgtggccgag
      601 cgcgtcgagg acggcggcaa gcgggtcggc taccgcatca ccggcagcaa gcagtggatc
      661 tccaacggcg gcgtcgccga cctgtacacg atcctcgcca acgccccggg tggcccgacc
      721 tggttcgtgg tcgacaaggg gacgccgggg ctcacgccgg gcaagcccga ggacaagcac
      781 gggatccgcg cgtcgaacac cgcggcgctc gcgctcgatc aggtctacgt cgacgccgat
      841 cgcgtggtcg gcggggccga aggccagggc ctggtccagg cccaggcggt gttcggctac
      901 acccggctga tggtcgccgc gttcggcctc ggcgccggct gggcggcgct gaaccgcgcg
      961 atcgagtact cgacgacccg gatccaggcc ggcacgccgc tggcccagaa gcagggctac
     1021 acccacaagc tgatcgtccc gcacgcggcg cggctggagg cggcgcgcgc ctacatcgag
     1081 gaggtcggcg tgcggctcga cgccggcgag gacccgctcc acaccgaggg cgcgatcgcc
     1141 aagtacctcg cgaccgaggc cggcaacgcg gcggccgacg cgtcgatcca ggcgctcggc
     1201 ggctacggct acacccgcga gtacatggtc gagaaggtca agcgcgatgt ccggatcacc
     1261 acgatctacg agggcaccag cgagatcatg gagatgacga tcgcgcgcga tcgctggcag
     1321 acccacctga agacccgcgg ccagcactac cacgaggcgg cggcccagct ggaggctctg
     1381 caccgccggc ggcccgaggt cggcgcgaac atcgcggcgc tcgcggcgca tgcgctgtcc
     1441 gacgtgctcg agcgcgcgcg gctggcccgg ctgacccgcc accagcacat cctgctccgg
     1501 ctcggcgagc tgatcgcgtg gt
//
LOCUS       DASVOR010002406         2309 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_115133,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002406 DASVOR010000000
VERSION     DASVOR010002406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115133"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(74..979)
                     /locus_tag="VF516_44250"
     CDS             complement(74..979)
                     /locus_tag="VF516_44250"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEX8114846.1"
                     /translation="MKNYRVIAAGIAGIIVLIGGILLAASLTTIHPGHVGVSVKKCGG
                     GGVSSNPIPTGYYWRSLFCEDVVEYPTSVQTLVLSHSPHEGHANDESITVTSSEGLPV
                     NLDVSVSFTLDPAKVPAVYTKYRNDISVIAHNFVRQTIREGLQSIFAQFTAEQLYSTK
                     REESRLEVQAFLTQHLSAEGFVIQQFTVNETRVPDAVVQAINAKVAMIQESQKAEAQV
                     RKTEAEAKQRVAQAQGEAEAKKLTADAEAYFNKTVAASITPEYVQYRALEKWNGELPQ
                     MMGTGAVPFVNVGGLKPAQQPQPQK"
     gene            complement(989..1246)
                     /locus_tag="VF516_44255"
     CDS             complement(989..1246)
                     /locus_tag="VF516_44255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114847.1"
                     /translation="MLAIDIALLVGGAVVLVVAAAVVGGRFGPRLEVRRRRLQGERNA
                     EQEKRRLAELCTICGTPIDPAHDLWDADQWWHRGCYRESLR"
     gene            1460..>2309
                     /locus_tag="VF516_44260"
     CDS             1460..>2309
                     /locus_tag="VF516_44260"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEX8114848.1"
                     /translation="MTGATGVVGRRLVPMLRARGDDVTAVVRSPAQAAALVRAGARPI
                     AVDLFDARVVRDAMVGHDAVINLATHIPKAARMFLPGAWRENDRLRRIASARLVDAAL
                     ALDVPRFVQESFAPVYADHGDAWIGEDEPLAPAAHTRTVVDAERAVARFSAAGRAGVV
                     LRFAAFYGPDSRFLADMVRLIERGWSPLPGAPEAFISSISHDDAATAAFAALDLPAGT
                     YNATDDEPLRHREFVDSLADALGVPHPRALPAWVATLGGSTARTMARSLRISNARLRA
                     AGWAPRY"
BASE COUNT          306 a          814 c          815 g          374 t
ORIGIN      
        1 cggggcgtga cgtcgatccg ggctgacgtg gcgctgcaat accagcggct cgccgcgtga
       61 ccccgccgcc gcgctacttc tggggctgcg gctgctgcgc cggcttgaga ccgccgacgt
      121 tgacgaacgg aaccgcgccg gtgcccatca tctgcggcag ctcgccgttc cacttctcga
      181 gcgcgcggta ctgcacgtac tccggcgtga tcgatgcggc gacggtcttg ttgaagtagg
      241 cctcggcgtc ggcggtgagc ttcttggcct cggcctcgcc ctgggcctgg gcgacccgct
      301 gcttggcctc ggcctcggtc ttgcggacct gggcctcggc cttctgcgac tcctggatca
      361 tggcgacctt ggcgttgatc gcctggacca ccgcgtccgg gacccgggtc tcgttgaccg
      421 tgaactgctg gatcacgaag ccctcggcgc tcaggtgctg ggtgaggaac gcctggacct
      481 cgagccggct ctcctcacgc ttggtcgagt acagctgctc ggcggtgaac tgggcgaaga
      541 tgctctgcag gccctcgcgg atggtctggc ggacgaagtt gtgggcgatc acgctgatgt
      601 cgttgcggta cttggtgtag accgccggga ccttggcggg gtcgagcgtg aacgacaccg
      661 agacgtcgag gttgaccggc aacccttccg acgacgtgac cgtgatcgac tcgtcgttgg
      721 catgaccctc gtgcgggctg tgcgacagca ccagggtctg gacgctggtc gggtactcga
      781 cgacgtcctc gcagaacagg ctgcgccagt agtagccggt cgggatcggg ttcgacgaca
      841 cgccgccgcc gccgcacttc ttcaccgaca cgccgacgtg gccggggtgg atggtggtga
      901 gcgacgcggc cagcaggatg ccgccgatca ggacgatgat accggcgatg cccgccgcga
      961 tgacccggta attcttcatg acgttctcct agcgcaagga ctctcgataa cacccccggt
     1021 gccaccactg gtccgcgtcc cacagatcgt gggccggatc gatcggtgta ccgcatatgg
     1081 tacatagctc tgcgagccgg cgcttctcct gctcggcgtt gcgctcgccc tgcaggcggc
     1141 ggcggcgcac ttccagccgg gggccgaacc gcccgccgac caccgcggcg gcgaccacga
     1201 gcaccacggc gccgccgacc agcaacgcga tatcaatggc gagcatgtgc ctcgcttccc
     1261 gagtgctgtc tagcaccgcc cgcgcgccga agacatacag tccgcatcaa gatggagcac
     1321 gccgccggtg gccgcaccgc ggcagtcgct ggagcaacgt aatccctgtt cccggccgcg
     1381 gcaagcgcgc cacatcagcg gatctccgag gacattccgg ggcatctggt ttgcaagacc
     1441 ctgcggcatg ttgatcttca tgaccggagc gaccggcgtc gtcggacggc ggctggtccc
     1501 gatgctgcga gcgcgcggag atgacgtgac cgcggtcgtt cgatccccgg cccaggcggc
     1561 ggcgctggtc cgcgccggtg cccggccgat cgcggtcgac ctgttcgatg cccgggtggt
     1621 gcgcgacgcg atggtggggc acgacgcggt gatcaacctg gcgacccaca tcccaaaggc
     1681 cgcgcggatg ttcctgcccg gcgcctggcg cgagaacgac cggctgcggc ggatcgcgtc
     1741 ggcgcggctg gtcgacgccg cgctcgcgct ggacgtcccg cggttcgtcc aggagtcgtt
     1801 cgcgccggtc tacgccgatc acggcgacgc ctggatcggg gaggacgagc cgctcgcgcc
     1861 ggcggcccac acccgcaccg tcgtcgacgc cgagcgcgcg gtcgcccggt tcagcgccgc
     1921 cgggcgcgcc ggcgtggtgc tgcggttcgc ggcgttctac gggccggact cgcgcttcct
     1981 ggccgacatg gtccgtctga tcgagcgcgg ctggtcgccg ctgcccggcg cgccggaggc
     2041 gttcatctcg tcgatctcgc acgacgacgc cgcgaccgcc gcgttcgccg cgctcgacct
     2101 cccggcgggg acctacaacg cgaccgacga cgagccgctg cggcaccgcg agttcgtcga
     2161 ttcgctggcc gacgcgctcg gcgtgcctca tccccgggcg ttgccggcgt gggtcgcgac
     2221 gctcggcggg tcgaccgcgc gcaccatggc gcgctcgctg cggatctcca acgcccggct
     2281 gcgggcggcc gggtgggcgc cccggtacc
//
LOCUS       DASVOR010002407         1515 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_434828,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002407 DASVOR010000000
VERSION     DASVOR010002407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434828"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1515)
                     /locus_tag="VF516_44265"
     CDS             complement(<1..>1515)
                     /locus_tag="VF516_44265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8114849.1"
                     /translation="QLVSAHRYTAGERLGRFVRRHRAAVMIAVLAAIGFAAGTTFAVR
                     RIIAERDVADQARQLADARRRAAERLIDTMLSDVKDRLQQIGRVDLLASLGTEIRDYY
                     ATLGSIPGGMPADDIDRMAAAIELLGVAERESGKLDKALATWTEARATLARIVTGRTG
                     EPARFERRLIARLDYELGTIHQQRGATEEAIRLYTQAEHELDALLAELPTDRAALLAS
                     ADIRDRLGDLLRNQGKVDQALDAYTEAKAERERAGGPGSRPQEEVLALSTSHLKLGSV
                     FQIRGESARALDEYRAAHRLRQGLLVAQRDDVALQNKLLEVELPLGDLQRQLGDPTGA
                     IETYRSALPMITALTRRDPDNTTWQRLRGNFEADFGFALMDTGEYRAGLAELDTAIEV
                     QRALIGRDPKSAIWLGDLSRSYTRAGDAELAVGELDHGIAHYQLGLDVRKELVAKNPA
                     SAPYRRSLGWSYAKLARGHTLRGDPARAIEAHEQALALRTALVAEVPDQREYRS"
BASE COUNT          169 a          546 c          580 g          220 t
ORIGIN      
        1 cgctgcggta ctcgcgctga tccgggacct cggcgaccag cgcggtgcgc agcgcgagcg
       61 cctgctcgtg ggcctcgatc gcgcgcgccg gatcgccgcg cagggtgtgg ccgcgggcga
      121 gcttggcgta cgaccagccc agcgaccggc ggtacggcgc cgatgccggg ttcttggcga
      181 ccagctcctt gcggacgtcg agcccgagct ggtagtgcgc gatgccgtgg tcgagctcgc
      241 cgaccgcgag ctcggcgtcg ccggcgcggg tgtagctgcg cgacaggtcg ccgagccaga
      301 tcgcgctctt gggatcgcgg ccgatcagcg cgcgctggac ctcgatcgcg gtgtcgagct
      361 cggcgagccc cgcccggtac tcgccggtgt ccatcagcgc gaagccgaag tcggcctcga
      421 agttgccgcg cagccgctgc cacgtcgtgt tgtccggatc gcgccgggtc agcgcggtga
      481 tcatcggcag cgcgctgcgg taggtctcga tcgccccggt cggatcgccg agctggcgct
      541 gcaggtcgcc gagcggcagc tcgacctcga gcagcttgtt ctggagcgcc acgtcgtcgc
      601 gctgggccac gagcaggccc tggcgcagcc ggtgcgcggc gcggtactcg tcgagcgcgc
      661 gcgccgattc gccccggatc tggaacaccg agccgagctt gaggtggctg gtcgacagcg
      721 ccagcacctc ctcctgcggc cggctgcccg ggccgccggc gcgctcgcgc tcggccttgg
      781 cctcggtgta ggcgtcgagc gcctgatcga ccttgccctg gttgcgcagc aggtcgccga
      841 gccggtcgcg gatgtcggcc gacgcgagca gcgccgcgcg gtcggtcggc agctccgcga
      901 gcagcgcgtc gagctcgtgc tcggcctggg tgtacagccg gatcgcctcc tcggtcgcgc
      961 cgcgctgctg gtggatcgtg ccgagctcgt agtcgagccg cgcgatcagc cggcgctcga
     1021 accgcgcggg ctcgccggtc cgcccggtca cgatccgcgc cagcgtcgcg cgtgcctcgg
     1081 tccaggtcgc gagcgccttg tcgagcttgc cggactcgcg ctcggcgacg ccgagcagct
     1141 cgatcgcggc cgccatccgg tcgatatcgt cggcgggcat cccgcccggg atgctgccga
     1201 gcgtggcgta gtagtcgcgg atctcggtgc ccaggctggc gagcaggtcg acgcggccga
     1261 tctgctgcag gcggtccttg acgtcggaca gcatggtgtc gatcagccgc tcggcggcgc
     1321 ggcgccgggc gtcggcgagc tggcgcgcct gatcggcgac gtcgcgctcc gcgatgatcc
     1381 ggcggaccgc gaacgtcgtg ccggcggcga acccgatcgc cgcgagcacc gcgatcatca
     1441 ccgcggcgcg gtggcggcgg acgaaccggc cgagccgctc gccggcggtg tagcggtgcg
     1501 ccgacaccag ctggc
//
LOCUS       DASVOR010002408         2990 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_553115,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002408 DASVOR010000000
VERSION     DASVOR010002408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2990
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553115"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VF516_44270"
     CDS             <1..190
                     /locus_tag="VF516_44270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEX8114850.1"
                     /translation="IISVGSVGQPRDYDNRASYTIYDTEERTFEFKRVAYDIDGAAQK
                     IFAADLERNFGNRLFLGV"
     gene            complement(191..>2990)
                     /locus_tag="VF516_44275"
     CDS             complement(191..>2990)
                     /locus_tag="VF516_44275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114851.1"
                     /translation="AADTDWLDQGRRALAEFDYETARAHFERAMQDSNGDPTAARSLL
                     ELLVDHMAADSDALALEDQLAPHAAADPEVRALLAVAASRMGNAAAASRLLEGLVGPR
                     VAEAWKALAEDAARRQADDGLGHCIARLTEADPTHPDLVRFRDQAQRLRADARKPAEL
                     ELAQLAEAGDDEATEARARALLARWPDDEAMLALLEPHRSLIASVARMNELQAEARGR
                     IAARRRIAAVRALDQARLALARGDLETCERLCEQADGRDLDRALRPALDQVRQELRAR
                     QELKRGLAMVDHLVASGDLVGARHELEVLLAADRVEAAHAVALRRRLDDLRAELRRAW
                     CVCANDVGEQPVHHDLVGELLGRLPYAEAALPWLAPGGRELVIASTEGPHLFLGRVAV
                     DDGRLRERRYLRAPVPLGELISATVDGDALWLIGDAGRVLQVSRMTGEPVRWLSLDPF
                     LTGGEQIERGFVASGGKHLWLEVSLPGGSPLDRVIEIEPGRLRRELPAARWFQPLVAG
                     SSSGVIGTGLDGGAVRYTERGAVVEEIAAGARMRVSAVVIDPDRELVVVGSRLDGAGE
                     IEIVRTRAGRTIHRRTLVATSSEAAHRCVSAREPGLLAVHHHVEQDDVEQDDVEQDGI
                     EHRDVQLAVFRFADPELIPLYATPAPWDLTLAQDADAAQVVCLWDSARGVEIERLGSQ
                     PPAFGDAIGLHARWFAPLLDGYFSCGTSVDGGENTAPISAANRAAARGDWHEVRTLLE
                     GVPPDSVTPEWLAHHCHLLGIAWLRTGAEAARARSLWQLGGSQERASDRLFTCRLDVC
                     LELVEPLPDPLPDAWWGPGASLVRQLRGAIASADRLTAAGQPGAALEVMRRRVVTGNP
                     ELQSAARLAAAWLAVAPRSTDDCFSAAIALARFVALAAGRAPGPPALDLPIEGAWSRD
                     QLTSLAERAKQWLTAWQA"
BASE COUNT          421 a         1118 c         1050 g          401 t
ORIGIN      
        1 catcatctcg gtcggctcgg tcggccagcc ccgcgactac gacaaccgcg cgagctacac
       61 gatctacgac accgaggagc gcaccttcga gttcaagcgc gtcgcctacg acatcgacgg
      121 cgcggcccag aagatcttcg cggccgacct cgagcgcaac ttcggcaacc gcctgttcct
      181 cggcgtctga tcaagcctgc cacgcggtga gccactgctt cgcacgctcg gccagcgagg
      241 tcagctgatc ccggctccag gccccttcga tcggaaggtc gagggccgga gggcccgggg
      301 cgcgacccgc ggcgagcgcg acgaaccggg ccagcgcgat cgccgcgctg aagcagtcgt
      361 cggtgctgcg gggggcgacc gccagccagg ccgcggcgag ccgcgccgcg ctctgcagct
      421 cggggtttcc cgtcaccacc cgacgacgca tcacctcgag agccgccccg ggctggcccg
      481 ctgccgtgag ccggtccgcg ctcgcgatgg ctcctcgcag ctgccggacg agcgacgctc
      541 cgggacccca ccacgcatcg ggtagcggat cgggcagcgg ttcgacgagc tcgaggcaca
      601 catcgagccg gcacgtgaag agtcggtcgc ttgctcgttc ctgggaacct ccgagctgcc
      661 agagagagcg cgcgcgggcg gcctcggctc cggtccgcaa ccatgcgatc cccaggaggt
      721 ggcaatgatg cgcaagccat tccggcgtca cgctgtcggg cggcacgcct tcgagcagcg
      781 tacggacctc gtgccagtcg ccgcgagcag cggcgcgatt cgcggcggag atcggcgccg
      841 tgttttcccc gccgtccacg ctggttccgc acgagaagta gccgtcgagc aacggcgcga
      901 accaccgcgc gtgcagcccg atcgcgtcgc cgaaggccgg cggctgcgat ccgaggcgct
      961 cgatctcgac gccgcgcgcg ctgtcccaga gacagaccac ctgagccgcg tcggcgtcct
     1021 gcgcgagggt gaggtcccat ggcgccgggg tcgcgtagag cggaatgagc tctggatcgg
     1081 cgaaccggaa cactgctagc tggacgtcgc gatgctcgat gccgtcctgc tcgacgtcgt
     1141 cctgctcgac gtcgtcctgc tcgacgtgat gatggacggc gaggagcccg ggctcccgcg
     1201 cggaaacgca gcgatgcgcc gcctcgctgg acgtcgcgac cagcgtccga cggtggatcg
     1261 tgcgtcccgc cctggttcgc acgatctcga tctcgccggc gccgtcgagc cgcgatccca
     1321 ccacgaccag ctcgcggtcg ggatcgatga ccaccgcgga cacccgcatc cgtgcgccgg
     1381 cggcgatctc ctcgaccacg gcgccgcgct cggtgtagcg caccgcgccg ccgtcgaggc
     1441 ccgttccgat gacccccgag gagctgccgg cgacgagcgg ctggaaccag cgggcggcgg
     1501 ggagctcccg gcggagccgc cctggctcga tctcgatcac ccggtccagg ggcgagccgc
     1561 cggggaggct cacctcgagc cagaggtgct ttcccccgga cgccacgaac ccgcgctcga
     1621 tctgctcgcc gccggtcagg aacgggtcca gggacagcca tcgaaccggc tctcccgtca
     1681 tccgcgacac ctggagcacg cgacccgcat cgccgatgag ccagagcgca tcgccatcga
     1741 ccgtcgcgga gatcagctcg cccaggggta cgggagctcg caggtacctt cgctcgcgca
     1801 accggccgtc gtccaccgcg acgcgcccga ggaacaggtg aggcccctcg gtgctcgcga
     1861 tcacgagctc gcgcccgccg ggggccagcc atggcagggc cgcttcggca tagggcagtc
     1921 ggccgagcag ctcgcccacc aggtcgtggt ggacgggttg ctcgccgacg tcgttggcgc
     1981 agacgcacca ggcgcgccgc agctccgcgc ggagatcgtc gagccgccgg cgcaacgcga
     2041 cggcatgagc ggcctcgacc cgatcggccg cgagcaggac ctccagctcg tgtcgagcgc
     2101 cgaccaggtc gcccgacgcg accaggtggt ccaccatggc cagcccgcgc ttgagctcct
     2161 gccgcgcgcg gagctcctgc cgcacctgat cgagggccgg gcgcagcgcg cggtcgagat
     2221 ctcggccatc tgcctgctcg cacaggcgct cgcaggtctc gagatcgcct cgggcgagcg
     2281 cgagccgcgc ctggtcgagc gcgcggaccg cggcgatgcg ccggcgagcc gcgatcctgc
     2341 cgcgggcctc ggcctggagc tcgttcatcc gggccacgct ggcgatcagc gagcggtgcg
     2401 gttcgagcag cgcgagcatg gcttcgtcgt cgggccagcg ggcgagcagc gctcgcgcgc
     2461 gggcttcggt ggcctcgtcg tcaccggctt cggccagctg cgccagctcc agctcggccg
     2521 gcttccgggc atcagcgcgt agccgttgcg cttggtctcg gaaccgaacg agatcggggt
     2581 gcgtcggatc ggcctcggtc aggcgagcga tgcagtgccc gagtccgtcg tccgcctgac
     2641 gcctggccgc atcctccgcg agggccttcc acgcctccgc gacccgggga cccaccaggc
     2701 cttccagcag gcgactcgcc gccgctgcgt tccccatgcg cgatgccgcg accgccagca
     2761 aggcgcgaac ctcggggtcg gcggcggcgt gcggcgccag ctgatcctcg agtgccaggg
     2821 cgtcactgtc cgccgccatg tggtcgacca gtagctcgag caacgagcgg gcggccgtcg
     2881 gatctccatt cgagtcctgc atcgcgcgct cgaagtgggc tcgcgccgtc tcgtagtcga
     2941 actcggccag cgcgcggcgc ccctggtcga gccagtcggt gtccgcggcc
//
LOCUS       DASVOR010002409         2449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_405370,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002409 DASVOR010000000
VERSION     DASVOR010002409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405370"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VF516_44280"
     CDS             complement(<1..442)
                     /locus_tag="VF516_44280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8114852.1"
                     /translation="MQRARRATRGFFLLCGIGAASWAPMVPFARARLALDEAQLGVVL
                     LCMGLGSAITMPLAGWVSHRHGNQLVLPISGALVCVAVPLLALAPSTSLLGAALALFG
                     AALGVLDVAMNAHAVDVERLHGRPLMSGFHALFSLGGLAGSAAMS"
     gene            683..1669
                     /locus_tag="VF516_44285"
     CDS             683..1669
                     /locus_tag="VF516_44285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114853.1"
                     /translation="MKTQRPGATGAGGGREATTAGATPGKRTLVEDLYDDSAPSAPVG
                     QALDASGGPQIEGGTLFSGPDPSLTGAVPTEGVATPDVSDADYESTLGVKAAIDAKKM
                     LPVEGINGKTFTATGCAGKKDGKVTFTFDRAFVGDYEYAAAGKTVRGVHVIISAALSG
                     CGEHTDVKLIQILRNFTKKDGKAVPADPKNDVRRKRSGWDDDKAPSRGWRVDGLTDKS
                     TPFYVDNSTTSDIYGNHGSSTKAAKLRDTPGDWSTDRNVGKEFRTCAVSYAGGKGTVL
                     ACVEWGYYIDDAGKATFYPATPVAHTGSVTEFTDAATRWDGIEGNTKANLVK"
     gene            1666..2082
                     /locus_tag="VF516_44290"
     CDS             1666..2082
                     /locus_tag="VF516_44290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114854.1"
                     /translation="MIRAGTVALIMLGLGACSNPARKETPLMNETQNATDDERLIARV
                     RAAVEARVPAEGLAAFVGQGPLIDTTAGGSLLALQRIDFAPDPEHPDERIDRDKARAV
                     VYWRRPVPGDDPRVVGVQIGDVGLARMFFAIILPPD"
     gene            complement(2095..>2449)
                     /locus_tag="VF516_44295"
     CDS             complement(2095..>2449)
                     /locus_tag="VF516_44295"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HEX8114855.1"
                     /translation="LRDGSERFDDNGIGTHGSASVTSIVHGTSHGCHRLYNQLAVRLA
                     DFLLRHRDHAVRGEQPVLYRRVVFHNDERFVANIKTRGFLYELTPPVPINVLKGNIRS
                     DRKVPPRDSAPARPD"
BASE COUNT          426 a          815 c          856 g          352 t
ORIGIN      
        1 cgctcatcgc cgccgagccg gccagcccgc cgaggctgaa cagcgcgtgg aagcccgaca
       61 tcagcggccg gccgtgcagc cgctcgacgt cgaccgcgtg cgcgttcatc gccacgtcga
      121 gcacgccgag cgccgcgccg aacaacgcca gcgccgcgcc gagcaggctc gtcgacggcg
      181 cgagcgccag gagcgggacc gccacgcaga ccagcgcgcc gctgatcggc aggaccagct
      241 ggttgccgtg gcggtggctg acccagccgg cgagcggcat cgtgatggcg gagccgaggc
      301 ccatgcacag cagcacgacg ccgagctgcg cctcgtcgag cgccagccgc gccctggcaa
      361 acggcaccat cggcgcccag ctcgccgcgc cgatcccgca caagaggaag aagccccggg
      421 tggcgcgacg ggcccgctgc acggtcttga gcgttggctc tagcaacgtg cataaatata
      481 gataatcgtg cacgaacgtg caagcgcgtg tttttgttgt ggatgaccgt tcttattcaa
      541 tctttcagct ccgggccaag aactgctgca gtgcggcgac gctgaggggc ctacggaacg
      601 aagggcggta aggggccgcc gccggaggcc ttcggttgtg cagagcatgt cccttcgcta
      661 ccgtggatcg cggggaggcg ccgtgaaaac ccaacgacca ggggcgaccg gagcaggtgg
      721 gggacgcgag gcgacgaccg ccggcgcgac gcctggcaag agaaccctcg tcgaggatct
      781 gtacgatgac tcggcgccct ctgcgccggt cggtcaggcg ctcgatgcca gcggcggccc
      841 gcagatcgag ggcggcacct tgttcagcgg ccccgacccc agtctgaccg gcgcggtgcc
      901 caccgaaggt gtcgccacgc ccgacgtcag cgacgccgac tacgagtcca cgttgggggt
      961 gaaggcggcc atcgatgcca agaagatgct tccggtcgag ggcatcaacg gcaagacgtt
     1021 caccgccacg ggctgcgcgg gcaagaagga cggcaaggtc acgttcacct tcgatcgtgc
     1081 gttcgtcggt gactacgagt acgcggccgc gggcaagact gtgcgcggcg ttcacgtgat
     1141 catctcggcg gcgctgagcg gctgcggtga acacaccgat gtcaagctga tccagatctt
     1201 acgcaacttc accaagaagg acggcaaggc ggtcccggcc gatcccaaga acgatgtgcg
     1261 ccgcaaacgc tcgggatggg acgacgacaa ggcgccatcg cgaggctggc gggtcgacgg
     1321 tctgaccgac aagtccacgc cgttctatgt cgacaacagc acgaccagcg acatctacgg
     1381 caaccacggc agctcgacca aggctgccaa gttgcgcgat acgcccggcg actggtccac
     1441 cgaccgcaac gtcggcaagg agtttcgtac ctgtgccgtg tcctatgccg gtggcaaggg
     1501 caccgtcctt gcttgcgtcg agtggggcta ctacatcgac gatgcaggca aggcgacgtt
     1561 ctaccccgcg acgccggtgg cgcacaccgg ctcggtgacc gagttcaccg acgccgccac
     1621 acggtgggac ggcatcgagg gcaacaccaa agcgaacctg gtcaaatgat ccgcgcgggc
     1681 accgtcgcgc tgatcatgct cggcctgggc gcctgctcga atccagcacg gaaggagacc
     1741 ccgctgatga acgaaacgca gaacgcgacc gatgacgagc ggctgatcgc gcgcgtccgc
     1801 gccgcggtcg aggctcgggt tcccgccgag ggtctggccg cgttcgtcgg ccagggaccg
     1861 ctcatcgata cgaccgccgg tggctcgctc ctggcgctgc agaggatcga cttcgcgccg
     1921 gatcctgaac atcccgacga gaggatcgac cgggacaagg cccgcgcggt cgtctactgg
     1981 cgtcgtccgg tccccggcga tgatccgcgc gtggttggcg ttcagatcgg cgatgtcggc
     2041 ctcgcgcgca tgttcttcgc gatcatcctc cccccggact gacggtcgcg cgcgtcagtc
     2101 cgggcgcgcc ggcgccgagt cgcgcggagg gaccttgcgg tcgctgcgga tgttgccctt
     2161 gagcacgttg atcggcaccg gcggggtcag ctcgtagagg aacccgcgcg tcttgatgtt
     2221 ggcgacgaac cgctcgtcgt tgtggaacac gacgcggcgg tagagcacgg gctgctcgcc
     2281 gcggaccgcg tggtcgcggt ggcgcagcag gaagtcggcg agccggaccg cgagctggtt
     2341 gtacagccgg tggcagccgt ggctcgtgcc atggacgatc gaggtgaccg acgccgagcc
     2401 gtgggtgccg atgccgttgt cgtcgaaccg ctcgctgccg tcgcgcagc
//
LOCUS       DASVOR010002410         1899 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_347214,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002410 DASVOR010000000
VERSION     DASVOR010002410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347214"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="VF516_44300"
     CDS             complement(<1..424)
                     /locus_tag="VF516_44300"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEX8114856.1"
                     /translation="MDTVTQRQLMVERTIRRRGVRDRRVLAAIAAVPREAFLPPELAE
                     FAYDDRPLPIEAGQTISQPYIVALMAEALQIGSDEDVLEIGTGSGYAAAVLARIARRV
                     YTIERHAELADLARERLSRLGFLNVEVQHGDGTLGWGDR"
     gene            complement(557..1003)
                     /locus_tag="VF516_44305"
     CDS             complement(557..1003)
                     /locus_tag="VF516_44305"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEX8114857.1"
                     /translation="MGFRKIVCPIDFSACSREALRVAVELARDGAATLVLAHVWEPPK
                     WFAGEVMVAPEVIQDVIDAEEAELERWKADAKQLGAGEVDARFLTGVAWDAIVELAKD
                     DRTIDLVVMGTHGRTGLKHVLLGSVAEKVVRHAPCAVLVVRDRERS"
     gene            1165..1773
                     /locus_tag="VF516_44310"
     CDS             1165..1773
                     /locus_tag="VF516_44310"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEX8114858.1"
                     /translation="MRTVSRAGVIAISLVVALLVIGGSCVATYNRLVRLDQAVQAQWG
                     QVQNTYQRRADLIPNLVATVKGAAAFERDTLTQVTQARSRVGQLALPPDVTSRPAEFQ
                     RYQQAQDQLGGALSRLLVVAEAYPNLTATQAFRDLQAQLEGTENRIAVERMRYNEAAR
                     EFDTARETFPTSIVAGMFGDRFVAKQYFTARPGSEALPEVKF"
BASE COUNT          279 a          689 c          672 g          259 t
ORIGIN      
        1 cgcgatcgcc ccagccgagc gtgccgtcgc cgtgctggac ctcgacgttg aggaagccga
       61 gccgggacag ccgctcgcgc gccaggtcgg cgagctcggc gtggcgctcg atcgtgtaga
      121 cccggcgcgc gatccgggcg agcacggcgg ccgcgtagcc cgagccggtg ccgatctcca
      181 gcacgtcctc gtccgatccg atctgcaggg cctcggccat cagcgcgacg atgtacggct
      241 gggagatcgt ctggccggcc tcgatcggca gcggccggtc gtcataggcg aactctgcga
      301 gctcgggcgg caagaacgcc tcgcgcggca cggcggcgat cgcggcgagg acgcggcggt
      361 cgcggacgcc ccggcggcgg atcgtgcgtt cgaccatgag ctggcgctgg gtgacggtgt
      421 ccatgcgccg gggtagagca agcggcacgc caggtgccgg gctagaaacc gaacgcgctc
      481 cggctgtgga tcagcaagcc aatgatcacg atcgcggcga tcagcagcac cagggtcttc
      541 agcgacgggt gcatggctag ctgcgctccc ggtcgcggac gaccagcacc gcgcacggcg
      601 catggcgcac gaccttctcg gcgaccgagc cgagcagcac gtgcttgagc ccggtgcggc
      661 cgtgggtgcc catcacgacg aggtcgatcg tccggtcgtc cttggccagc tcgacgatcg
      721 cgtcccacgc cacgccggtc aggaaccggg catcgacctc gccggcgccg agctgcttcg
      781 catcggcctt ccagcgctcg agctcggcct cctcggcatc gatcacgtcc tggatcacct
      841 cgggcgcgac catgacctcg ccggcgaacc acttcggcgg ctcccagacg tgcgcgagca
      901 cgagcgtcgc cgccccgtcc cgcgcgagct cgacggcgac ccgcagcgcc tcgcgcgagc
      961 aggcggagaa gtcgatcgga cacacgatct tgcggaatcc catgtcgaga tacggagcat
     1021 gcgacgtgcc ggcatctcgg ctgtcctcgc cacggttcga cgaccgatcg cgcgcaaccg
     1081 ctgcggtcga ctccgccgtc ggcgcagatg ctgcgccctc caccgtcggg tgttcatgga
     1141 ccgcagattg caacacctcg gggcatgcga accgtcagcc gcgccggagt catcgcgatc
     1201 tcgctcgtcg tcgcactgct cgtgatcggc gggtcgtgcg tcgccacgta caaccggctg
     1261 gtccggctcg accaggccgt ccaggcgcag tgggggcagg tgcagaacac ctatcagcgc
     1321 cgcgccgacc tgatcccgaa cctggtcgcc accgtcaagg gcgcggcggc gttcgagcgc
     1381 gatacgctga cccaggtgac ccaggcgcgg agccgcgtcg gtcagctcgc gctgccgccc
     1441 gacgtgacct cgcgcccggc cgagttccag cgctaccagc aggcgcagga tcagctgggc
     1501 ggcgcgctgt cgcgcctgct ggtcgtcgcc gaggcgtacc cgaacctgac ggcgacccag
     1561 gcgttccgcg atctgcaggc ccagctcgag ggcaccgaga accggatcgc ggtcgagcgc
     1621 atgcgctaca acgaggcggc ccgcgagttc gacaccgcgc gcgagacgtt cccgacgtcg
     1681 atcgtcgccg ggatgttcgg cgatcggttc gtcgcaaagc agtacttcac cgcgcggccc
     1741 ggcagcgagg ccctgcccga ggtcaagttc tgatggggct cgccgcgctc gcagtggtgg
     1801 tggcggtcgc cgcggccccg gcggcgcaac cggcgacgcc cgcgccgccg acccggtggg
     1861 tcgaggatca cgcggcgatg atgtcccccg cggcgcgcg
//
LOCUS       DASVOR010002411         2400 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_522770,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002411 DASVOR010000000
VERSION     DASVOR010002411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522770"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VF516_44315"
     CDS             <1..611
                     /locus_tag="VF516_44315"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEX8114859.1"
                     /translation="EWNGHDLIRFADAVRSIHLGDGETVTTSGASVIFADIVTSISGD
                     GPRVTAVAALGLIIMVVLLVGRNRRALAVLVATASGSLLMVAACAVLSLKVNFLDFVA
                     LPITLGIGIDYAINVAHRHDHEPDPVTTLRTSGSAVFVCSLTTIIGYGSLLISENLAI
                     RGFGMASLIGEIACVMTALILVPALLALNHKPAIGASESVAA"
     gene            complement(615..2129)
                     /locus_tag="VF516_44320"
     CDS             complement(615..2129)
                     /locus_tag="VF516_44320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114860.1"
                     /translation="MTLAAHDRKQLALEIRHPAGSAVTGGSHDVELFLFIPRNVGLTA
                     ANYPGAQFYHDLTAYLRLDLPDLGLEDLCASARSPLRRLDVHLAELALGEATPDPIGV
                     AVKLFGHGFTEAVHRERTRLSSLLQTAADRDARPGEPLTRIRELATASRTALERLREA
                     KHRFAPFRRVAPDVWAVFQQTDEYTSLFLDEALATLAHELGARPEEVDDTGALEVLAS
                     CAAAEAAYRHAQGFVNLDPTSGLNREYFLYRRSLLKKAVHQALWVRTRRRQVDSYLRN
                     TAGMVAAGMAATWALIAQVPAQFRSLSPAAQTLMFVLPVIGYVLKDRIKELTKEWMFR
                     RMRAYDQETELVGLQLARSGLGSLCGTLRERAHFVDQAPDDVLAIRQRGRWIAGARIG
                     GELVLAYRRKLEIQPPPDGQLHGSHELALRQIIRLSLRHFMARLDDRDQIVRHYDPST
                     GGFATHEIAKVYHLNAVVRITGQSHGAYLERWRVVFSRDGIERIERGERGERSG"
     gene            complement(2173..>2400)
                     /locus_tag="VF516_44325"
     CDS             complement(2173..>2400)
                     /locus_tag="VF516_44325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114861.1"
                     /translation="LEDSVFASVRAELESLAADHRDLARVPIRALIRGAIPDDARFHL
                     TALARPDAAPLWLLVLEGPDRDGMADGARHV"
BASE COUNT          334 a          877 c          846 g          343 t
ORIGIN      
        1 acgagtggaa cggccacgac ctgatccggt tcgccgacgc ggtgcgctcg atccacctcg
       61 gcgacggcga gaccgtcacg acctcgggcg ccagcgtgat cttcgccgac atcgtcacgt
      121 cgatctcggg cgacgggccg cgcgtcaccg cggtcgccgc gctcggcctg atcatcatgg
      181 tggtgctgct ggtcggccgc aaccggcgcg cgctcgccgt gctggtcgcg accgcgtcgg
      241 gctcgctgct catggtcgcg gcgtgcgccg tgctgagcct caaggtcaac ttcctcgact
      301 tcgtcgcgct gccgatcacg ctcgggatcg gcatcgacta cgcgatcaac gtcgcgcatc
      361 gccacgacca cgagcccgat ccggtcacga cgctgcgcac cagcggcagc gcggtgttcg
      421 tgtgctcgct gaccaccatc atcggctacg gctcgctgct gatcagcgag aacctggcga
      481 tccgcgggtt cggcatggcg tcgctgatcg gcgagatcgc ctgcgtcatg accgcgctga
      541 tcctggtccc cgccctgctg gcgctgaacc acaagccggc gatcggcgcc agcgagtcgg
      601 tcgccgcgta gcggtcatcc cgaccgctcg cctcgctcgc ctcgctcgat ccgctcgatc
      661 ccgtcgcggc tgaacaccac gcgccagcgc tcgaggtacg ccccgtggct ctggccggtg
      721 atgcggacca cggcgttgag gtgatagacc ttcgcgatct cgtgggtcgc gaacccgccg
      781 gtcgatggat cgtagtggcg gacgatctga tcgcggtcgt cgaggcgcgc catgaaatgg
      841 cgaaggctca gccggatgat ctggcgcagc gccagctcgt ggctgccgtg cagctgcccg
      901 tccggcggtg gctggatctc gagcttccgg cgatacgcca gcaccagctc gccgccgatc
      961 cgcgcgcccg cgatccagcg gccgcgctgc cggatcgcga gcacgtcgtc gggggcctga
     1021 tcgacgaagt gggcccgctc gcgcagggtg ccgcacagcg agccgagccc cgaccgggcg
     1081 agctgcaagc cgaccagctc ggtctcctga tcgtaggcgc gcatgcgccg aaacatccac
     1141 tccttcgtca gctccttgat ccggtccttg agcacgtatc cgatcaccgg cagcacgaac
     1201 atcagggtct gggccgccgg cgacagcgac cggaactgcg ccgggacctg ggcgatcaac
     1261 gcccacgtcg ccgccatccc ggccgcgacc atcccggccg tgttgcgcag gtagctgtcg
     1321 acctggcggc ggcgcgtgcg gacccacagc gcctgatgga ccgccttctt gagcagcgac
     1381 cgccggtaga ggaagtactc gcggttcaat cccgaggtag ggtcgaggtt gacgaagccc
     1441 tgcgcgtgcc ggtacgccgc ctcggcggcc gcgcagctcg ccagcacctc gagcgcgccc
     1501 gtgtcgtcga cctcctccgg ccgcgcgccg agctcgtggg cgagcgtggc gagcgcctcg
     1561 tcgaggaaca ggctggtgta ctcgtcggtc tgctggaaca ccgcccagac atcgggcgcg
     1621 acccggcgaa acggcgcgaa ccggtgcttg gcctcgcgca gccgctcgag ggccgtgcgc
     1681 gacgcggtcg cgagctcgcg gatgcgggtc aggggctcgc cgggccgcgc gtcgcgatcg
     1741 gcggccgtct ggagcaggga cgacagccgc gtgcgctcgc gatgcaccgc ctcggtgaac
     1801 ccgtggccga acagcttcac cgcgaccccg atcggatcgg gcgtcgcctc gcccagcgcg
     1861 agctccgcga ggtggacgtc gagccggcgc agcggcgagc gcgcgctcgc gcacagatcc
     1921 tcgaggccga ggtccggcag gtcgaggcgc aggtacgcgg tgaggtcgtg gtagaactgc
     1981 gcgcccgggt agttcgccgc cgtcagcccg acgttgcgcg ggatgaacag gaacagctcg
     2041 acgtcatggc tgccgccggt gaccgccgag cccgccgggt gccggatctc cagcgcgagc
     2101 tgcttgcgat cgtgcgccgc gagggtcacc cggggaccgc tcgaccgcgg agacctcgcg
     2161 cgcggtgcag ggtcagacat gccgcgcccc gtccgccatc ccgtcccggt ccggaccttc
     2221 gagcaccagg agccacagcg gcgcggcatc cggacgggcg agcgccgtga ggtggaaccg
     2281 cgcgtcgtcc gggatcgcgc cccggatcag cgcgcggatc ggcacccgcg cgaggtcccg
     2341 gtggtcggcc gccagcgact cgagctccgc ccgcacgctc gcgaacacgc tgtcctcgag
//
LOCUS       DASVOR010002412         4179 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_201875,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002412 DASVOR010000000
VERSION     DASVOR010002412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201875"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1641
                     /gene="asnB"
                     /locus_tag="VF516_44330"
     CDS             <1..1641
                     /gene="asnB"
                     /locus_tag="VF516_44330"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEX8114862.1"
                     /translation="DRHQFRSRADTEVIIHLYEERQVEVPQALDGMFAFGLWDAKRRR
                     LLLARDRMGKKPLYYTIAGGRLLFASELKALLGHPDVARRLDPVALNEYLTFSNVPEP
                     RTLFEGIRKLPAGHTLVCDPQGNLTIARYWSPLDGPAWISPASRGEAVERVRDLVKRA
                     VKKRLMADVPVGAFLSGGVDSSTNVALMSRLVSTPLQTFTVEFTGFGPAENFHDVPYA
                     RQVASMFGCRHTEVQVTAAEALAYVPEMVQHQDEPLGDPACLPMHFVSRAARAAGLKV
                     VLVGEGSDEVFCGYPEFPRLLETYNGKWSQLRRMPQLLRRVVQRGAELAGAPSGRVDV
                     LRRAAEDEPLYLGLDVVFFDWEKEQLYTEEGRRRMPRKAVDTIAAYYREIAERRPDAD
                     FSQQMSYVELRNRLPELLLMRVDKFSMQHSLEARAPFLDHTLATYALSLPAAMKIDGT
                     RTKAVLKDAAHDWLPDDVIERTKQGFRVPLPAWLRGELAPWAEDLLRRSPLHKLGIFN
                     FDHILELWRRHKDGVADHSFDLWCLINLSGWYEHWFAT"
     gene            1638..2873
                     /locus_tag="VF516_44335"
     CDS             1638..2873
                     /locus_tag="VF516_44335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEX8114863.1"
                     /translation="MKNGAHDDPQRAGNGARSVLYIMDTLGLSGRTKGYIDLALHLDP
                     ARYRPHFCSLGEESSELVERLHERAIPVEVLPLAAGLRPDGVWKLAQLMRRRGIDVVH
                     PINPRPLLYAGLAARLCRVHSAVGSLSAFACQVPDRAYKFLPQELVNRGRRQALRNQM
                     ACGLMNHVVTVSDELGERFCKFTAEAAGPLAGIGYRALRRRMHTISYGIDLGPYRAVT
                     AAEVAELRARVGATADTVLVGSVGRLVEQKDYPTQLRAFALAARADPRLRMVLAGDGP
                     LRSAIETHAAELGISDRLVLLGHWVRVPALLKALDVFVLASKFEPYGVALLEAKCAGS
                     AIVATAVNEIPKLLRDGVTGLIVPAESPEPMAKAFLRMAQDAALRRSLATAAYAEAEN
                     KHSIDAVARAYQALYDTPS"
     gene            2896..>4179
                     /locus_tag="VF516_44340"
     CDS             2896..>4179
                     /locus_tag="VF516_44340"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8114864.1"
                     /translation="MIGTYARRPAAEAELSAMLDALAPRAPDAATTWQAPDGAARFGF
                     RWLRTQPGEHNPGVLVAPDGNLAMACDGHVFADDGKQGPAPLLERFAAKGPEGWRGLD
                     AQFGLAIWDARRGRLTLGRDPLGVRFVYYWSSADGVIFASEIKALLRHPAVTRGFDEI
                     ALVQYLVFLTSPGPRTLFAGVRRVPAGGVVELSATGEAGERRWWDLLDDAVDERDDEG
                     YYVNRTRELHQAAVRRRSVEGPIGALCSGGNDSSSNVVLLSRQGANPLHTFTVGLAEF
                     EGDTKYNDLYFARQVAEYAKSQHHESLLSTDEFINLVPQTVEAQDDLVSEPSSVFLFH
                     ALRMAKDQGVRVVVTGEANDELCCGHGGMVAIRDGHKARWQPLMRLPRPLRGAAAALA
                     PLVGLARRAGRRARRREVLRQPDARAAPRGGAAAQG"
BASE COUNT          573 a         1529 c         1488 g          589 t
ORIGIN      
        1 gatcgccacc agttccggtc gcgcgccgac accgaggtga tcatccacct ctacgaggag
       61 cgccaggtcg aggtcccgca ggcgctcgac gggatgttcg cgttcgggct gtgggacgcc
      121 aagcggcggc ggctcttgct ggcgcgcgac cggatgggca agaagccgct gtactacacg
      181 atcgccggcg gccggctgct gttcgcatcc gagctcaagg cgctgctcgg ccaccccgac
      241 gtcgcgcgcc ggctcgaccc ggtggcgctc aacgagtacc tgacgttctc caacgtcccc
      301 gagccgcgca cgctgttcga aggcatccgc aagctgccgg ccggccacac gctggtgtgc
      361 gatccgcagg gcaacctgac gatcgcgcgc tactggtcgc cgctcgacgg cccggcctgg
      421 atctcgccgg ccagccgcgg cgaggcggtc gagcgcgtcc gcgacctggt caagcgcgcg
      481 gtgaagaagc ggctgatggc cgacgtcccg gtcggcgcgt tcctgagcgg cggcgtcgac
      541 tcgtcgacca acgtcgcgct gatgagccgg ctggtgtcga ccccgctgca gaccttcacc
      601 gtcgagttca ccgggttcgg gccggccgag aacttccacg acgttccgta tgcgcgccag
      661 gtggcgtcga tgttcggctg ccgccacacc gaggtccagg tcaccgcggc cgaggcgctg
      721 gcgtacgtgc ccgagatggt ccagcaccag gacgagccgc tcggcgatcc ggcctgcctg
      781 ccgatgcact tcgtgtcgcg cgccgcgcgc gcggccggcc tcaaggtcgt gctggtcggc
      841 gagggcagcg acgaggtgtt ctgcggctac cccgagttcc cccggctgct cgagacctac
      901 aacggcaagt ggtcgcagct ccggcgcatg ccccagctgc tgcgccgggt ggtccagcgc
      961 ggcgccgagc tcgccggcgc gccgagcggc cgggtcgacg tgctccggcg cgccgccgag
     1021 gacgagccgc tgtacctcgg gctcgacgtg gtgttcttcg actgggagaa ggagcagctg
     1081 tacaccgagg agggccggcg ccggatgccg cgcaaggcgg tcgacaccat cgcggcctac
     1141 taccgggaga tcgccgagcg gcggccggac gccgacttca gccagcagat gtcgtacgtc
     1201 gagctgcgca accggctgcc cgagctcctg ctgatgcggg tcgacaagtt ctcgatgcag
     1261 cactcgctcg aggcgcgcgc gccgttcctc gaccacacgc tcgcgaccta cgcgctgtcg
     1321 ctgccggccg cgatgaagat cgacggcacc cggaccaagg cggtgctcaa ggacgcggcg
     1381 cacgactggc tgcccgacga cgtcatcgag cgcaccaagc aggggttccg cgtgccgctg
     1441 ccggcgtggc tgcgcggcga gctcgcgccg tgggccgagg acctgttgcg gcgctcgccg
     1501 ctgcacaagc tcggcatctt caacttcgac cacatcctcg agctgtggcg gcgccacaag
     1561 gacggcgtcg ccgaccactc gttcgacctg tggtgcctga tcaacctgtc cggctggtac
     1621 gagcattggt tcgcgacatg aagaacggag cccacgacga tccccagcgc gccggcaacg
     1681 gggcgcgctc ggtgctgtac atcatggaca cgctcggcct gtccgggcgg accaagggct
     1741 acatcgacct cgccctgcac ctcgatccgg cgcgctaccg gccgcacttc tgctcgctcg
     1801 gcgaggagtc gagcgagctg gtcgagcggc tgcacgagcg cgcgatcccg gtcgaggtcc
     1861 tgccgctcgc ggccgggctg cggcccgacg gcgtgtggaa gctcgcccag ctgatgcgcc
     1921 ggcgcggcat cgacgtcgtc cacccgatca acccgcggcc gctgctgtac gccgggctgg
     1981 ccgcgcggct gtgcagggtc cactcggcgg tcggctcgct cagcgcgttc gcctgccagg
     2041 tccccgatcg cgcgtacaag ttcctgcccc aggagctggt caaccgcggc cgccgccagg
     2101 cgctgcgcaa ccaaatggcc tgcgggctga tgaaccacgt ggtcacggtg tccgacgagc
     2161 tcggcgagcg gttctgcaag ttcaccgccg aggccgccgg gccgctggcc ggcatcggct
     2221 accgcgcgct gcgccgccgg atgcacacga tcagctacgg catcgacctc gggccgtacc
     2281 gcgcggtcac cgccgccgag gtcgccgagc tccgggcccg ggtcggcgcc accgccgaca
     2341 ccgtgctggt cggcagcgtc ggccggctcg tcgagcagaa ggactacccg acccagctgc
     2401 gcgcgttcgc cctggcggcg cgcgccgatc cgcggctgcg catggtgctc gccggcgacg
     2461 gcccgctgcg cagcgcgatc gagacccacg ccgccgagct cgggatctcc gaccgcctgg
     2521 tgctgctcgg ccactgggtc cgcgtgccgg cgctgctcaa ggcgctcgac gtgttcgtgc
     2581 tggcgtcgaa gttcgagccg tacggcgtgg cgctgctcga ggccaagtgc gccgggagcg
     2641 cgatcgtcgc gaccgcggtc aacgagatcc ccaagctgct gcgcgacggg gtgaccgggc
     2701 tgatcgtgcc ggccgagtcg cccgagccga tggccaaggc gttcctccgg atggcgcaag
     2761 acgctgcgct ccggcgctcc ctggccaccg cggcctacgc cgaggccgag aacaagcaca
     2821 gtatcgacgc cgtcgctcgc gcctaccagg cgttgtacga tactccttcg tgaatccatg
     2881 tcggccatct gtggaatgat cggaacgtac gcccggcgcc ccgccgccga ggccgagctg
     2941 tccgccatgc tcgacgcgct ggcgccccgt gcgcccgacg ctgcgacgac ctggcaggct
     3001 cccgacggcg ccgcccggtt cggcttccgc tggctgcgca cgcagcccgg cgagcacaac
     3061 ccgggcgtgc tggtcgcgcc cgacggcaac ctggcgatgg cgtgcgatgg ccacgtgttt
     3121 gccgacgacg gcaagcaggg cccggcgccg ctgctcgagc ggttcgcggc caagggcccc
     3181 gagggctggc gcgggctcga cgcccagttc ggcctcgcga tctgggacgc ccgccgcggc
     3241 cggctgaccc tggggcgcga tccgctcggc gtgcgcttcg tctactactg gagctcggcc
     3301 gacggggtga tcttcgcgtc cgagatcaag gcgctgctcc gccaccccgc ggtgacccgc
     3361 ggcttcgacg agatcgccct ggtccagtac ctggtgttcc tgacctcgcc cgggccgcgc
     3421 acgctgttcg ccggggtccg ccgggtcccg gccggcggcg tggtcgagct gtccgccacc
     3481 ggcgaggccg gcgagcggcg gtggtgggac ctgctcgacg acgcggtcga cgagcgcgac
     3541 gacgagggct actacgtcaa ccggacccgc gagctgcatc aggccgcggt ccgccggcgc
     3601 agcgtcgagg gaccgatcgg cgccctgtgc tcgggcggca acgactcgag ctccaacgtc
     3661 gtgctgctgt cgcgccaggg cgcgaacccg ctccacacct tcaccgtcgg gctcgccgag
     3721 ttcgagggcg acaccaagta caacgacctg tacttcgcgc gccaggtcgc cgagtacgcc
     3781 aagagccagc accacgagtc gctgctgtcg accgacgagt tcatcaacct ggtgccgcag
     3841 accgtcgagg cccaggacga cctggtgtcc gagccgtcga gcgtgttcct gttccacgcg
     3901 ctgcgcatgg ccaaggacca gggcgtgcgg gtcgtggtca ccggcgaggc caacgacgag
     3961 ctgtgctgcg gccacggcgg catggtcgcg atccgcgacg gccacaaggc gcgctggcag
     4021 ccgctgatgc gactgcccag gccgctgcgc ggggccgcgg cggcgctcgc gccgctggtg
     4081 ggacttgctc gacgcgccgg tcgacgagcg cgacgtcgag aagtactacg tcaaccggac
     4141 gcgcgagctg caccacgcgg cggtgcggcg gcgcaaggt
//
LOCUS       DASVOR010002413         2536 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_143204,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002413 DASVOR010000000
VERSION     DASVOR010002413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143204"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..752
                     /locus_tag="VF516_44345"
     CDS             177..752
                     /locus_tag="VF516_44345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114865.1"
                     /translation="MNTFSRVAIAMVGMTGLAVAQPKTDAPKAADAKAGQDMGPPAEL
                     AAVAKASAGTWHCKGQGLDHTMKMVDTTGTMTVKLDVNNWWLHSAFDSKMGKEPFHFE
                     SFSTFNPATKRWTRVMVESGGHWATGESAGMKDNKVDWHLTTHSPMMGDAMFRDHEDL
                     SDPKAGAKMWGEFSPDQGKTWTKVYEVSCKK"
     gene            complement(775..1755)
                     /locus_tag="VF516_44350"
     CDS             complement(775..1755)
                     /locus_tag="VF516_44350"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEX8114866.1"
                     /translation="MSHRFAGRRVAVVMGGLSAEREVSLNTGAGVLAALLERGWDAVA
                     IDWAPGTSLARLLEASGAQVVWNALHGTYGEDGAVQGLCACLQIPCTGSGVLASALAM
                     DKVMSKRIFESNGLPTPRWRLLPHDPGPGTGPGTGPGTGPGTSPGDAAALAGWPLPYV
                     VKPANEGSSVGVSIVDTPDQRDAALALARTFHGPVIIEEYIAGTEVFVGILNDRVLGS
                     VEVRPATRFYDYEAKYKRTDTRYLIPPELPARVIERTQDIALAAYRALGCTGHARPDL
                     RISTAGEPFVLEVNTLPGLTKTSLLPKIARSIGMDYPTLCEHILETASTI"
     gene            complement(1758..>2536)
                     /gene="murB"
                     /locus_tag="VF516_44355"
     CDS             complement(1758..>2536)
                     /gene="murB"
                     /locus_tag="VF516_44355"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEX8114867.1"
                     /translation="AEVASLARWCAAAGVPITVVGGGSNLIVRDGGIRGVVLATRRLR
                     GLALAPPVTIQVEAGVSTGKVLSLALQHELGGVEFLGGVPGSVGGGMIMNAGTYLGEF
                     KDVTTRVDTVRLADGELIRRDRAACGFRYRGSDLPASEVVVAAELVLRPRPRAEIQAE
                     VAALRKRRAEREPKKVASNGSTFKNPPGDFAGRLIEVAGCKGWREGDAVCSPVHANWL
                     VNTGRATAAELTRLIERVRAEVARVHGIHLDLEVKIIGED"
BASE COUNT          376 a          910 c          902 g          348 t
ORIGIN      
        1 gaggggagtg gccacgtcca taggaaccgc ggagcgtcgt gaggtttcgg tgcaacgata
       61 ccgcggctcg cgctgggttc cggcgccgcg tccgctgtaa cggaacacgg gaggcgccga
      121 tgttgcgatc tcgcgtttga gtatagtgcg cgggttttca cccaggagca tgccagatga
      181 acacgttctc gcgcgtggcc atcgcgatgg tcgggatgac cggcctcgcc gtggcgcaac
      241 ccaagaccga cgcccccaag gcggccgacg ccaaggccgg gcaggacatg ggcccgccgg
      301 ccgagctcgc cgccgtcgcc aaggcgagcg ccggcacctg gcactgcaag ggccagggcc
      361 tggatcacac catgaagatg gtggacacca ccggcacgat gacggtcaag ctcgacgtca
      421 acaactggtg gctgcacagc gccttcgatt cgaagatggg caaggagccg ttccacttcg
      481 agtcgttctc gacgttcaac ccggccacca agcggtggac gcgcgtgatg gtcgagagcg
      541 gcggccactg ggccaccggc gagtcggccg gcatgaagga caacaaggtc gactggcatc
      601 tcaccacgca ctcgccgatg atgggcgacg ccatgttccg cgatcacgag gacctgtcgg
      661 atcccaaggc cggcgcgaag atgtggggcg agttctcgcc cgaccagggc aagacctgga
      721 ccaaggtcta cgaggtgagc tgcaagaagt gacggccgag gcgaggccgc cgcgctagat
      781 cgtcgacgcg gtctccagga tgtgctcgca cagcgtcgga tagtccatcc cgatgctgcg
      841 cgcgatcttg ggcagcaggc tggtcttggt cagtccgggc agcgtgttga cctcgagcac
      901 gaacggctcg ccggcggtcg agatccgcag gtcgggccgc gcgtggccgg tgcagccgag
      961 cgcgcggtac gcggcgagcg cgatgtcctg cgtccgctcg atcacgcgcg ccggcagctc
     1021 cggcgggatc aggtaccggg tgtcggtccg cttgtacttg gcctcgtagt cgtagaaccg
     1081 ggtcgccggc cggacctcga ccgagccaag cacgcggtcg ttgaggatgc cgacgaacac
     1141 ctcggtcccg gcgatgtact cctcgatgat caccgggccg tggaacgtcc gggcgagcgc
     1201 gagcgcggcg tcgcgctggt ccggcgtatc cacgatgctg accccgaccg agctgccctc
     1261 gttggccggc ttgaccacgt acggcagcgg ccagccggcc agcgccgcgg cgtcaccggg
     1321 gctcgtgccg gggcctgtgc cggggcctgt gccggggcct gtgccggggc ctggatcgtg
     1381 cggcaacagg cgccagcgcg gcgtcggcag gccgttgctc tcgaagatcc gcttggacat
     1441 caccttgtcc atcgccagcg cgctcgcgag cacccccgac ccggtgcacg ggatctgcag
     1501 gcacgcgcac agcccctgga ccgcgccgtc ctcgccgtag gtcccgtgca gcgcgttcca
     1561 caccacctgg gcgcccgacg cctcgagcag gcgggccagg ctggttccgg gcgcccagtc
     1621 gatcgcgacg gcgtcccagc cgcgctcgag cagcgcggcc agcacgcccg cgccggtgtt
     1681 gagcgacacc tcgcgctcgg cggacagccc gcccatgacg acggcgacgc ggcgacctgc
     1741 gaagcgatgc gacatgccta gtcttctccg atgatcttga cctcgaggtc gaggtggatg
     1801 ccgtggaccc gggcgacctc ggcgcgcacg cgctcgatca gccgggtcag ctcggcggcg
     1861 gtggcgcggc cggtgttgac cagccagttg gcgtggaccg gcgagcacac cgcgtcgccc
     1921 tcgcgccagc ccttgcagcc ggcgacctcg atcagccggc cggcgaagtc gccgggtggg
     1981 ttcttgaacg tcgagccgtt gctcgcgacc ttcttcggct cgcgctcggc gcgccgcttg
     2041 cgcagcgccg cgacctcggc ctggatctcg gcgcgcggcc gcggccgcag caccagctcg
     2101 gcggcgacca cgacctcgct cgccggcagg tcgctgccgc ggtagcggaa cccgcacgcc
     2161 gcgcggtcgc gccggatcag ctcgccgtcg gcgagccgca cggtgtcgac ccgggtcgtg
     2221 acgtccttga actcgccgag gtaggtcccg gcgttcatga tcatgccgcc gccgaccgag
     2281 ccgggcacgc cgccgaggaa ctcgacgccg ccgagctcgt gctgcagcgc cagcgacagc
     2341 accttgccgg tcgacacccc ggcctcgacc tggatcgtca ccggcggcgc cagcgccagg
     2401 ccgcgcagcc gccgggtcgc gagcaccacg ccgcggatcc cgccgtcgcg cacgatcagg
     2461 ttggagccgc cgccgaccac ggtgatcggc accccggccg ccgcgcacca gcgcgccagg
     2521 gatgccacct cggccg
//
LOCUS       DASVOR010002414         1942 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_522805,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002414 DASVOR010000000
VERSION     DASVOR010002414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522805"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF516_44360"
     CDS             <1..244
                     /locus_tag="VF516_44360"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8114868.1"
                     /translation="THFGMTVEEAWLGVTRHAARALGLGGTSGTISRGARGDVLVWDC
                     DDPAHVPYRYGAATRLIDAVFVAGERLAAALSPLAS"
     gene            complement(261..890)
                     /locus_tag="VF516_44365"
     CDS             complement(261..890)
                     /locus_tag="VF516_44365"
                     /inference="COORDINATES: protein motif:HMM:NF019247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HEX8114869.1"
                     /translation="MTSFRSTRPVLALLALAVTTSAALAQPKPAPPAPPAPPKDTQAK
                     FEPPSGPGAGQAFLKQFEGEWTVERNFYPPGGGAPNHATGECTQKMMQDGRFLQSDFT
                     FHQDGKTTTGTGISGFDPQTGLFTTFWYDSRSTKFSIRQSRDPFDGKQIVLYSASLAA
                     THGQEHQSRTVSHLEDGGRKLIHQQFNQGADGKEHLLMELILVKKPARK"
     gene            complement(1366..>1942)
                     /locus_tag="VF516_44370"
     CDS             complement(1366..>1942)
                     /locus_tag="VF516_44370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HEX8114870.1"
                     /translation="DTGEMISGGNFHGQPVALALDAAAMAVAELANISERRIEQLVNP
                     HLSSGLPPFLAPDSGLHSGFMIAQVTAAALVSENKILAHPASVDSIPSSAGREDHVSM
                     GATAALKLAIIHDHVRTVLAIELLCGAQGIDLRRPLTTTRPLEAIHAAIRLRVPAMPV
                     DRPIAPDIAAVRALIDDGSLLAAARAAGANP"
BASE COUNT          283 a          634 c          717 g          308 t
ORIGIN      
        1 cacccacttc gggatgaccg tcgaggaggc ctggctcggc gtcacgcgcc acgccgcccg
       61 cgcgcttggc ctcggcggga cgagcggcac gatcagccgc ggcgcgcgcg gcgacgtcct
      121 ggtgtgggac tgcgacgacc cggcccacgt cccgtaccgc tacggcgccg cgacccggct
      181 gatcgacgcg gtgttcgtcg ccggcgagcg gctcgccgcc gcgctgtccc cgctggcgag
      241 ctgaccggcc accggccggc tcacttgcgg gccggcttct tgaccaggat cagctccatc
      301 agcaggtgct ccttgccgtc ggcgccctgg ttgaactgct ggtggatcag cttgcgcccg
      361 ccgtcctcga ggtgagacac cgtgcgcgac tggtgctcct ggccgtgggt cgccgccagc
      421 gacgcgctgt acagcacgat ctgcttgccg tcgaacggat cgcggctctg ccggatcgag
      481 aacttggtcg accgcgaatc gtaccagaac gtcgtgaaca gcccggtctg cggatcgaac
      541 ccgctgatcc cggtgccggt cgtcgtcttg ccgtcctggt ggaacgtgaa gtccgattgc
      601 aggaaccggc cgtcctgcat catcttctgg gtgcactcgc cggtcgcgtg gttgggcgcg
      661 ccgccgcccg gcgggtagaa gttgcgctcg acggtccatt cgccctcgaa ctgcttcagg
      721 aacgcctgtc cggcgcccgg acccgacggc ggctcgaact tggcctgggt gtccttcggc
      781 ggcgcgggcg gcgcgggcgg cgcgggcttg ggctgcgcga gcgcggcgct ggtggtcacg
      841 gcgagcgcga gcagcgcgag aaccgggcgc gtggatcgaa acgaggtcat gtcatctccc
      901 ttctggcgga acatcgtcgc tctgcgagcg cgatgagtcg atagacgcaa gcggtggttg
      961 acgttgacgg tttgacgtcg acgtgaaccc agaacgttga ggttgagggc agcgaagcga
     1021 tacgtagacg tctgacccca ccgtcgacct cgacgtaacc tcgacgtaac ctcgacgtcg
     1081 acctcagaag atcttcgtcc acatcgcgac gatgctggtc agcaagtcga tgccgcgctc
     1141 ctagaggtag ttcgagtcaa cgtgcaaggt ccagggtggg gtcagacgtc tacgttgccg
     1201 tcgacgtcca gggtggggtc aaggtccagg tccacgtcga cgtccagggc gccgatgacg
     1261 gctacacgag cagcatcgtg gtgtaaccgg cacattcgac gcgagccgcg caatgcgtct
     1321 cgaatcgcca cttcttcaca gctcgccagc ccgcgacgag cacactcacg ggttcgcgcc
     1381 ggccgcgcgg gcggcggcga gcagcgagcc gtcgtcgatc agggcgcgga ccgcggcgat
     1441 gtcgggggcg atcgggcggt cgaccggcat cgccgggacg cgcagacgga tcgcggcgtg
     1501 gatcgcctcg agcggccggg tcgtggtcag cggccggcgc aggtcgatgc cctgggcgcc
     1561 gcacagcagc tcgatcgcga gcacggtgcg gacgtgatcg tggatgatgg cgagcttgag
     1621 cgcggcggtc gcgcccatcg agacgtggtc ctcgcggccg gccgacgacg ggatcgagtc
     1681 gaccgaggcg gggtgggcca ggatcttgtt ctcggacacc agggcggcgg cggtgacctg
     1741 ggcgatcatg aagccggagt gcagcccgga gtcaggggcc aggaacggcg gcaggccgct
     1801 ggacaggtgc gggttgacca gctgctcgat ccggcgctcg ctgatgttgg cgagctcggc
     1861 gacggccatg gcggcggcgt cgagcgcgag cgcgaccggc tggccgtgga agttgccgcc
     1921 gctgatcatc tcgccggtgt cg
//
LOCUS       DASVOR010002415         3538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_231173,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002415 DASVOR010000000
VERSION     DASVOR010002415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231173"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2245)
                     /locus_tag="VF516_44375"
     CDS             complement(<1..2245)
                     /locus_tag="VF516_44375"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheR family methyltransferase"
                     /protein_id="HEX8114871.1"
                     /translation="MSVAVVGAGEAVSVEELLRGLPDALGFALVLASPLSPEAVRSAC
                     RVPVVAVDRRTSVEHGRIYAVPVDHDVRFVQGHLIRGDERRAIAWFDALLRSVADEIG
                     ARTTAVVLAGHGDDGALGIQRVKQAGGWTLAEDAGGELGEMPRAAIATGLVDLVLPID
                     QLAARLAISAAPPGTDPRDADPDAAEVADALGDIVALIRAGSGHDVGTYRRATLVRRI
                     ARRMQICESATFAAYRDHLRERPSELAHLVRDLLLRTTQFFRDPETFERVACDVVPGL
                     FSGKRAGDPVRIWIAGCSTGEEAYSLGILLLEHAARLRPAPQIQVFATDVDDQALAFA
                     RQGCYPASIALDVAPARLQRFFVRDRGRYRVHDELRELALFAPHSVLRDPPFSRLDLI
                     SCRNLLVDLEPDAQRRALSRFHFGLRGDGVLLLGGSDSAEDPRLFGVVDRDHRVFARL
                     ASPAPAGPFDRTGRRRPPSDAEAAPSIDELHTPPDASSSEPAIRELQDELHRTRERLR
                     ITVEQYEASLEELKASNEELQAINDELRYATSELETSQDELQSINEELGALNQELRCK
                     IDELGRAHSDLQNLMTSTEIGVVFLDRELRIQRFTPRAQDVFNIIPSDLGRPLAHLTH
                     RLADEAPCQDACQEMWQVAQSVLDTLRPVERELHGRDGRCFLARWLPYRSIENRIDGV
                     VLTFVDVTDLRDAVAARRRSEQALQQVEERLQVALHSAPLAALGFERDEPGGIGAPAD
                     ADSGALVA"
     gene            complement(2371..2805)
                     /locus_tag="VF516_44380"
     CDS             complement(2371..2805)
                     /locus_tag="VF516_44380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8114872.1"
                     /translation="MLTESARGTGRRVLVVDDHPDSADASCMLLSALGYECRPAVTGE
                     NALAEVERFQPDVVICDIGLPDISGYDIARTLRSRHGSSLYLAALTGWDQPSARARAL
                     AAGFDEHLVKPANARALRAVMVASMQRRVLLALGTGEYPTVR"
     gene            3231..>3538
                     /locus_tag="VF516_44385"
     CDS             3231..>3538
                     /locus_tag="VF516_44385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114873.1"
                     /translation="MAGACRNNRDQNKGQSPNAMPTEQNQTPGGTSGGTAGGTGQTGG
                     ATGGTGTTSGGATGGTGTSGSGGQAGETGATGTSGTSAGTSGGTSGGTSGGTAGTGSP
                     S"
BASE COUNT          586 a         1246 c         1245 g          461 t
ORIGIN      
        1 tcgcgaccag cgcgccggag tccgcatcgg ccggcgcgcc gatccccccc ggctcatcac
       61 gctcgaagcc gagcgcggcg agcggcgccg agtgcagcgc gacctgcagc cgctcctcga
      121 cctgttgcag cgcctgctcg ctgcggcgcc gcgcggcgac cgcgtcgcgc agatcggtca
      181 cgtcgacgaa ggtcagcacg acgccgtcga tccggttctc gatcgagcga tacggcagcc
      241 agcgcgccag gaagcaccgg ccgtcgcggc cgtgcagctc gcgctcgacc gggcgcagcg
      301 tgtccagcac cgactgcgcg acctgccaca tctcctggca cgcgtcctgg cacggcgcct
      361 cgtccgccag gcgatgcgtg aggtgggcca gcgggcgacc gaggtcggac gggatgatgt
      421 tgaacacgtc ctgagcgcgc ggggtgaacc gctggatgcg gagctcgcga tccaggaaca
      481 ccacgccgat ctcggtcgat gtcatcaggt tctggagatc ggagtgggca cgaccgagct
      541 cgtcgatctt gcaccgcagc tcctggttga gcgcgccgag ctcctcgttg atcgactgga
      601 gctcgtcctg gctggtctcc agctccgagg tcgcgtagcg cagctcgtcg ttgatcgcct
      661 ggagctcctc gttggacgcc ttgagctcct cgagcgaggc ctcgtactgc tcgaccgtga
      721 tgcgtaaccg ctcgcgggtg cgatgcagct cgtcctgcag ctcgcggatc gccggctcgc
      781 tcgacgacgc gtccggcggg gtgtggagct cgtcgatcga cggcgccgcc tcggcgtcgg
      841 acggtgggcg ccggcgcccg gtgcggtcga acgggccggc cggtgcgggc gaagccaggc
      901 gggcgaacac gcgatggtcg cggtcgacca cgccgaacag ccgggggtcc tccgcggagt
      961 ccgatccacc gagcagcagc acgccgtcgc cgcgcagccc gaagtggaac ctcgagagcg
     1021 cccggcgctg cgcgtcgggc tcgaggtcga ccagcaggtt gcggcagctg atcagatcga
     1081 gccgggagaa cggcggatcg cgcagcacgc tgtggggagc aaacagcgcg agctcgcgca
     1141 gctcgtcgtg aacgcgatac cggccgcggt cgcggacgaa gaaccgctgc agccgcgccg
     1201 gcgccacgtc gagcgcgatc gacgcggggt agcagccctg gcgggcgaac gccagcgcct
     1261 ggtcgtcgac atcggtcgcg aacacctgga tctgcggtgc cgggcgcagc cgcgcggcgt
     1321 gctccagcag caggatgccg agcgaatacg cctcctcacc ggtggagcat ccggcgatcc
     1381 agatccgcac ggggtcgccg gcgcgcttgc cggagaacag cccgggcacc acatcgcacg
     1441 cgacccgctc gaacgtctcc ggatcgcgga agaactgggt cgtccggagc agcaggtcgc
     1501 ggaccaggtg cgccagctcg ctcggtcgct cgcgcaggtg gtcgcggtac gccgcgaagg
     1561 tcgcggactc gcagatctgc atccggcgcg cgatccggcg caccagcgtc gcgcgccggt
     1621 acgtgccgac gtcgtgaccc gacccggccc ggatcagcgc gacgatgtcg cccagcgcgt
     1681 ccgcgacctc cgccgcatcc ggatccgcat cgcgcggatc ggtccccggc ggtgcggcgc
     1741 tgatcgccag tcgcgccgcg agctgatcga tcggcagcac gagatccacc aggccggtcg
     1801 cgatcgccgc gcgcggcatc tctccgagct cgccgccggc gtcctcggcg agggtccatc
     1861 caccggcctg cttgacgcgc tggatgccca gcgcgccgtc atcgccgtgc cccgcgagca
     1921 cgaccgcggt ggtgcgcgcc ccgatctcgt cggcgaccga gcgcaggagc gcgtcgaacc
     1981 aggcgatcgc gcggcgctcg tcgccgcgga tcagatgacc ctggacgaac cggacgtcgt
     2041 ggtccaccgg aacggcgtag atccggccat gctcgaccga cgtgcgccga tcgaccgcga
     2101 ccaccggcac gcggcacgcc gagcgtacgg cctccggcga cagcggtgac gccagcacga
     2161 gcgcgaatcc gagcgcgtct ggaaggccgc ggagcagctc ctcgacgctg accgcctccc
     2221 cggcaccgac gaccgcgacg ctcaaccccc cgtgatcggg caacatggca gaaaaaacag
     2281 accacggatc gccgagggcg ccaatgctcg tagaacagtt agttgcagca actgggcgtg
     2341 cgtcgaggcc acaccggaaa ctcaggcaaa tcaacgcacc gtcgggtact cgccggtccc
     2401 caacgcgagc agcacgcggc gctgcatcga tgccaccatc accgcgcgca atgctcgagc
     2461 gttcgctggc ttcaccaggt gctcgtcgaa ccccgccgcc agcgcacgcg cccgcgccga
     2521 cggctgatcc cagccggtga gcgcggcgag gtacagcgag ctaccatggc ggctccggag
     2581 ggtgcgtgcg atgtcatatc cgctgatgtc gggaagcccg atatcgcaga tcacaacgtc
     2641 gggttgaaac cgctccacct cagcaagcgc gttctcaccg gtcaccgccg gccgacactc
     2701 gtagccaagc gcggacagca gcatgcacga ggcgtcggcg gaatcggggt gatcatcgac
     2761 gacgaggaca cgacgaccag ttccacgagc agactcggtc agcatatacg ccacttcaac
     2821 atggagcggc tgggcgcgcc aagcgcggcg agaaaatact cgatgaaagt ttcctcatcg
     2881 gctcgattac cgtaaggcgc cctatctcgg tatcgccacg gggttcgtcc atcgacggca
     2941 gctgcgcgtc gacgtcacag cgatcgcgat ccgcatcgag ttgcgacggc cggccgtgcc
     3001 gcgctggagc acacgctcga gcctggcaaa gttccgtcga gcgcgagtat gcctcgctcc
     3061 gtgcgccgat gcgagtcgtg gcggcagcgc gcgtgaaaaa accggctgcc gacggcgcga
     3121 taccgaacac caggctcggc gcgccgtgca tggctcttgc tcgcctgtcg gtggaggagg
     3181 aacctatgac tcgttggatt tttctttcag cattcgtggt agctgcgacg atggccgggg
     3241 catgtcgcaa caatcgtgac cagaacaagg gccagtcgcc gaacgcgatg ccgaccgagc
     3301 agaaccagac tcccggcggg acgtccggag gaacggccgg cggtaccggc cagacgggcg
     3361 gtgcgaccgg cgggaccggg acgacgagcg gcggcgcgac cggtgggacc gggacatccg
     3421 gatccggggg acaggccggt gagaccggcg cgaccggcac atccggcacg agcgccggca
     3481 cgagcggcgg cacgagcggc ggcacgagcg gcggcacggc cgggaccggg agtccgtc
//
LOCUS       DASVOR010002416         4037 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_553214,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002416 DASVOR010000000
VERSION     DASVOR010002416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553214"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1096)
                     /locus_tag="VF516_44390"
     CDS             complement(<1..1096)
                     /locus_tag="VF516_44390"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8114874.1"
                     /translation="MSKQSLLLVDGDTRSLRVLEVSLRKAGFMVTAATSVQDALDKLE
                     LHAPDLIISETAFPDGDGFELRRRVRATPEWSEIPFIFLTAEAAIENKIRGLELGVDD
                     YLTKPIYIKEIVTRINILLQKRQRVRFEERRDGRTRFAGRVQDMPVVDVIQTIEISRK
                     SGVIQFVGERGRQAAIYFRDGRVIDAEAGSLQGEDAVYRLLTWSEGEFEVVFRTVRRR
                     EVITMSSQALLMEGMRRLDEWSRLFEQLPPLTHRFEVDTIELAARLGDVPDDNNRILR
                     LVDGKRTLLEVIDASDIGDLECMQAISRLYFEELLIDLDHGLEPGKAPRRDTGKHVAV
                     VEADGPAAGHDETSGPVAGEAAAQGGQAARE"
     gene            1587..2207
                     /locus_tag="VF516_44395"
     CDS             1587..2207
                     /locus_tag="VF516_44395"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEX8114875.1"
                     /translation="MTALAEALAALGLPATLEAPLADFAALFHKWNRRINLSAARSEH
                     ELHGHIVDCLHLVPLLRAAASAWPPPRPVPVLDVGSGGGLPAVVVAICLPDLRVTALE
                     PIHKKHAFLRTAARELALPTLEPLAERIEAHGRHDYAAAMSRATLDLRDWLLLGLRHV
                     APGGVVFGFEAIPRDDLPPGMLRHPYLHLGKPRAIVALPRPASPAT"
     gene            complement(2247..2399)
                     /locus_tag="VF516_44400"
     CDS             complement(2247..2399)
                     /locus_tag="VF516_44400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114876.1"
                     /translation="MNHLDNIAIRQRKSRVRDVVFAAFVALGALVSLGTVSTVANAAS
                     THIASR"
     gene            2660..3226
                     /gene="mug"
                     /locus_tag="VF516_44405"
     CDS             2660..3226
                     /gene="mug"
                     /locus_tag="VF516_44405"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF007570.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HEX8114877.1"
                     /translation="MKLTSAQRAAALRQRVPDVIAPRLRILFCGINPGLYSAAAGHHF
                     AGPSNRFWPTLHIAGFTPTRLRPADEAALLPLGLGVTNLVARTTSTAGHLSSEELRRG
                     ARRLTRKVLAFQPAILAILGLGAYRLAFAEPRAVGGLQKRTIGETRIWLLPNPSGLNA
                     HHSNAEFVQLFRTLRRYAALPPRASRRS"
BASE COUNT          578 a         1555 c         1254 g          650 t
ORIGIN      
        1 cctcccgcgc ggcctggccg ccctgagccg ccgcctcgcc cgcgaccggc cccgacgtct
       61 cgtcgtggcc cgcggccggt ccgtcggcct cgaccaccgc cacgtgcttc ccggtgtcgc
      121 gccgcggcgc cttgccgggc tcgagcccgt ggtcgagatc gatcagcagc tcctcgaagt
      181 acagccgcga gatcgcctgc atgcactcca ggtcgccgat gtccgacgca tcgatcacct
      241 cgagcagcgt gcgcttgcca tcgaccagcc gcaggatccg gttgttgtcg tcgggcacgt
      301 cgcccagccg cgccgccagc tcgatcgtgt cgacctcgaa gcgatgggtc agcggcggca
      361 gctgctcgaa cagtcgcgac cactcgtcga gccgccgcat gccctccatc agcagcgcct
      421 gcgagctcat cgtgatcacc tcgcgacgcc gcaccgtccg gaacaccacc tcgaactcgc
      481 cctcgctcca cgtcagcaag cgatacaccg cgtcctcgcc ctgcagcgac cccgcctccg
      541 cgtcgatcac ccggccgtcg cggaagtaga tcgccgcctg ccggccgcgc tccccgacga
      601 actggatcac gcccgacttc cggctgatct cgatcgtctg gatcacgtcg accaccggca
      661 tgtcctggac ccggccggcg aaccgcgtcc ggccgtcgcg gcgctcctcg aaccgcaccc
      721 gctgccgctt ctgcagcagg atgttgatcc gggtcacgat ctccttgatg tagatcggct
      781 tggtcagata atcgtcgaca ccgagctcca gcccgcggat cttgttctcg atcgccgcct
      841 cggcggtgag gaagatgaac gggatctccg accactccgg ggtcgcgcgc acccgccgcc
      901 gcagctcgaa cccgtcgcca tctggaaacg cggtctccga gatgatcagg tccggtgcat
      961 gcagctccag cttgtcgagc gcgtcctgaa ccgaggtcgc cgcggtcacc atgaagcccg
     1021 ccttgcgcag cgatacctcc agcacccgca ggctgcgagt gtccccgtcg accaggagca
     1081 gactttgctt cgacacggag atcgggatga agtgacgtgg gcgtgagacg gcgtgcaatc
     1141 gtatcgaact cgccaaaccg tcgccagaag cctagcctgg acgcggtgat cgagttggat
     1201 atgggcgcct gccctacccg ttcacccagg cgccggcgcg agcgccaccg ccacaacctc
     1261 gaccagggca ccgctctcgt cggtattgcc ccgatccggc acgcttccac gggagcgttt
     1321 tctcaccgaa cccgcttgcc gtccccgccc ccgggcgctt cgatcgcaag gatgtcgtgg
     1381 ccgcctcgac catgccggcc cgtcccgggc gaaccacgcc cgtgcggagt ccaccgagcc
     1441 gacctccgcg cggatctcgc aggccccatg acctgccggg ggcaccggca ctcccgcgtc
     1501 cggacggggt gcagcgatcc ggcgcgcttt cccccggacg gttcccgtca cgacggccac
     1561 cgaacgcgat ccgtcctccc gggctcatga ccgcgctcgc cgaggccctc gccgcgctcg
     1621 gcctcccggc gacgctggag gcaccgctcg ccgacttcgc cgcgctattt cacaaatgga
     1681 atcggcggat caacctgtcg gcggcgcgga gcgagcacga gttgcacggc cacatcgtcg
     1741 attgcctcca cctcgtgcct ctgctccgcg ccgccgcctc cgcatggccg ccaccccggc
     1801 cggttcccgt cctggacgtc ggctccggcg gcggccttcc ggccgtcgtc gtcgcgatct
     1861 gcctccccga cctgcgcgtc actgccctcg agcccatcca caagaagcac gccttcctcc
     1921 gcaccgccgc ccgcgagctc gcgctgccga ccctcgagcc gctcgccgag cgcatcgaag
     1981 cccacggccg ccacgactat gcagcggcca tgtcccgcgc gaccctcgat ctccgagact
     2041 ggctcttgct cggcctccgc cacgtcgccc caggcggtgt cgtgttcgga ttcgaggcaa
     2101 ttcctcgcga cgatctccca cccggcatgc tccgccatcc ctacctgcac ctcggcaagc
     2161 cccgcgcgat cgtggcgctt cctcggccgg cctctcctgc gacctgaccc gcgcggtgtc
     2221 ctcgccgggt cgctggcgcg ctccgctcag cggctcgcga tgtgcgtcga ggcggcgttc
     2281 gccaccgtcg acaccgtgcc gagcgaaacg agggcgccga gagccacgaa cgccgcaaag
     2341 accacatcac gaacgcgcga tttgcgctgc cgaatagcaa tgttatcgag gtgattcatc
     2401 gtttccgctc ctgctcagca gcgctccaat acaagaccga gaccaatttc aatgtaacga
     2461 tttcagctgg ttgcatgact attccccggc ctgcgacggt cgcgtcgacg ccgcagcctt
     2521 gccacctgtc gcagcacccc cgcagggctg ccgggccacg gctcgcgtcg gtcacagcct
     2581 ctgcgcgaac ctcgcgccga gcgccgccac taccgcagct ttccccgtaa aagtcgccca
     2641 ggccgcctgt gagtaacctg tgaagcttac ctccgcgcag cgcgcggccg cgcttcgcca
     2701 gcgcgtgccc gacgtcatcg cgccccgcct ccgcatcctg ttttgcggga tcaacccagg
     2761 cctctactcc gccgccgccg gacaccactt tgccggtccc agcaaccgct tctggccgac
     2821 gctccacatc gctggattca ctccgacccg gctgcgcccg gccgacgagg ctgcgctgct
     2881 tccgctgggc ctcggcgtca ccaacctcgt ggcccggacg acctccaccg ccggacacct
     2941 ctcgagcgag gagctgcggc gcggagcccg acggctcact cgcaaggtgc tggccttcca
     3001 gcccgcgatc ctcgcaatcc tgggtcttgg cgcctaccgc ctcgcgttcg cggagccacg
     3061 agcggtcggc ggcctccaga agcgcaccat cggcgagacg cgtatctggc tgctccccaa
     3121 ccccagcggg ctcaacgccc accactccaa cgccgagttc gtgcagctgt tccgcacctt
     3181 gcgacgctac gccgcgctac ctcctcgcgc ctccaggcgt agctagctcg ctggctctcc
     3241 gatttctgga gtggcgaggc tcctttgcag cctctgccgg acggcatgga cgacgccgcg
     3301 ctgccaatcg cgccctctcc ttggtgcctc gacgaacagc acctcgctga ccaagctcct
     3361 gcctcgccgc ggtggccccc cacgaggggg ctggtccgac gctgagcacg gcgctcggca
     3421 gtaccctccc ccagttgtcc tccgtttcac gtggaacttc gttgcgcggt gaggccggtg
     3481 cgggtccggg ccgggcatgg ggcgcgagaa ggcgatcacg ggcctcgagc gctgcgcttg
     3541 gtctcaggtg atcccgatcc cccacgtggg ggcgcctggt ccagcttcgc cgatcgtatc
     3601 gggggggtgt cggggactcg gcgacgtgcg attcgcccga ggtgccaaag agaagatgcg
     3661 atcgtcggcg cgggacaatg ccgtctggta gtttcacgtg gaacttcgtt gcgcggtgag
     3721 gccggcgcgg gtccgggccg ggcatggggc gcgagaaggc gatcacgggc ctcgagcgct
     3781 gcgctggtct caggtgatcc cgatccccca cgtggagacg gcgggtccag cttcgccgat
     3841 cgtatcgggg gcgggaggtc gggggactcg gcgacgtgcg attcgcccaa ggtgccaaag
     3901 agaaggtgcg atcgtcggcg ggacaatgcc gtccggtacg ttcacgtgaa cgtcgttgcg
     3961 cggtgaggcc ggcgcgggtc cgggccgggc atggggcgcg agaaggcgat cacgggcctc
     4021 gagcgctgcg cttggtc
//
LOCUS       DASVOR010002417         2482 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_115272,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002417 DASVOR010000000
VERSION     DASVOR010002417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115272"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="VF516_44410"
     CDS             complement(<1..629)
                     /locus_tag="VF516_44410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114878.1"
                     /translation="MIGWLIGCGDSNASNPIDAPGSIDAAIDAPSIDARTICDPLATF
                     GPPVPFTGAALPNSIESRPHLTSDELSVYFSAGGFLSGVSQLYAAHRSAVTAKFGAPN
                     LLPVVNMAFDRDPTVRSDGLELWFASNRGAPGGVLHLFVATRASTSDDFGTPAPDPYI
                     NTANPDVNDSTPFLTADWQQLWFASSRPPDPSGAFANNIWHAAREGRSFT"
     gene            747..2225
                     /locus_tag="VF516_44415"
     CDS             747..2225
                     /locus_tag="VF516_44415"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8114879.1"
                     /translation="MRLGIDFGTTRTVVAAVDDGRHPIAAFNDGGEFRDYLPGIAALR
                     GGELIVGWEAARALSDGRAVRHAIRSIKRAATELAPDDPVPGLPGVTALELVTAFLAE
                     LRRAIVERSNLDVDGPLEAMVAVPASAASRQRWLTLEAFRRAGFAPIGLVNEPTAAAI
                     EFAHRHLDLGRRSPKRYVVVYDLGGGTFDASAVSLEGRRFDLIASEGLARLGGDDFDE
                     LIVRAAGAEPTAAALERAREAKETLKATTRKLVVELGGEPVALDTAELYARAAPLIER
                     TIAQTDLLFERLRDRGIDPDDPRELGGLYLVGGGVGFPAVGRALRARFSRKLQLAAQP
                     FASTAVGLAIAADPGAHVMIREALTRHFGVWREAEAGADKVFDPLIGKNTSADAPLVV
                     RRSYRPTHAVGHLRFLECGALDAGGQPVQDLVPWRDVYFPYDPALADRGDLDGVASER
                     RPDLMANEIAETYTCASDGTISVVIENMSRGYARSYELGALR"
     gene            complement(2222..>2482)
                     /locus_tag="VF516_44420"
     CDS             complement(2222..>2482)
                     /locus_tag="VF516_44420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114880.1"
                     /translation="RPDVIYAAAMGKGGANRFGCSRVAQGMTERIGRISPADMASMTA
                     RVEQLAWMTLAGGWLAAFMVTAGVANFVRPYRTAWAASGDGR"
BASE COUNT          360 a          882 c          888 g          352 t
ORIGIN      
        1 gtgaagctcc tcccctcccg cgctgcgtgc cagatgttgt tcgcaaacgc accggatgga
       61 tcgggcggcc gcgacgaggc gaaccagagc tgttgccagt ccgcggtcag aaacggagtg
      121 gagtcattga cgtctggatt cgctgtgttg atgtaggggt ccggcgccgg cgtaccgaaa
      181 tcgtcggagg tggaggcccg cgtcgcgacg aagagatgca ggacaccccc gggtgccccg
      241 cgattcgacg cgaaccacag ctccagcccg tccgagcgca ccgtcggatc ccgatcaaac
      301 gccatgttca ccacagggag caggttggga gccccgaatt tcgcggtgac ggcgctgcga
      361 tgggcggcgt acagctggga gacgccgctg agaaatcccc ccgcgctgaa atacaccgag
      421 agctcatccg acgtcaggtg cggccggctc tcgatcgagt tgggcaaggc ggcgccggtg
      481 aacggcacgg gcggaccgaa cgttgccagc ggatcacaga tcgttctcgc atcgatggac
      541 ggcgcgtcga tcgccgcgtc gatcgaaccc ggtgcgtcga tcgggttcga tgcgttcgag
      601 tctccgcacc cgatcagcca gccgatcaag cacacccatg tcagtctcat cctcactcct
      661 tgccgtcgat acgctaccac aagcggcgcg atcccgcctc cgccacgaaa gtggcgccgc
      721 cggatcgggt ttggcaggct ggcggcatgc gactcggcat cgacttcgga acgacccgca
      781 ccgtcgtcgc ggcggtcgac gatggccgcc accccatcgc cgcgttcaac gacggcggcg
      841 agttccgcga ctacctcccc ggcatcgcgg cgctgcgcgg cggcgagctg atcgtcggct
      901 gggaggccgc gcgggcgctg agcgacggcc gtgcggtccg tcacgcgatc cggtcgatca
      961 agcgcgcggc gaccgagctt gcgcccgacg atccggtgcc cggcctgccg ggggtgaccg
     1021 cgctcgagct ggtgacggcg ttcctcgccg agctgcggcg cgcgatcgtc gagcgctcga
     1081 acctcgatgt cgacggaccg ctcgaggcga tggtcgcggt gccggcgagc gcggcgtcgc
     1141 gccagcgctg gctgacgctc gaggcgttcc ggcgcgccgg gttcgcgccg atcgggctgg
     1201 tcaacgagcc gaccgccgcg gcgatcgagt tcgcgcatcg ccacctcgac ctcggccggc
     1261 gatcgcccaa gcgctacgtc gtggtctacg acctcggcgg ggggacgttc gatgcgtcgg
     1321 cggtgtcgct cgagggccgg cggttcgatc tgatcgcgtc ggaggggctc gcccggctcg
     1381 gcggcgacga cttcgacgag ctgatcgtgc gcgcggccgg tgccgagccg acggcggccg
     1441 cgctggagcg ggcgcgcgag gccaaggaga cgctcaaggc gacgacccgc aagctggtgg
     1501 tcgagctcgg gggcgagccg gtggcgctgg acaccgccga gctgtacgcg cgcgccgccc
     1561 cgctgatcga gcgcacgatc gcgcagaccg acctgctgtt cgagcggctg cgcgatcgcg
     1621 gcatcgatcc cgacgacccg cgcgagctcg gcgggctgta cctggtcggc ggcggggtcg
     1681 ggttccctgc ggtcggacgg gcgctgcgcg cgcggttcag ccggaagctc cagctcgcgg
     1741 cgcagccgtt cgcgtcgacc gcggtcgggc tcgcgatcgc cgccgatccc ggcgcgcacg
     1801 tgatgatccg cgaggcgctg acccgccact tcggcgtgtg gcgcgaggcc gaggccggcg
     1861 ccgacaaggt gttcgacccg ctgatcggca agaacaccag cgccgatgcg ccgctggtgg
     1921 tgcgccgcag ctaccgcccg acccatgcgg tcggccacct gcggttcctg gagtgcgggg
     1981 cgctcgacgc cggcggccag ccggtgcagg acctcgtgcc gtggcgcgat gtctacttcc
     2041 cgtacgatcc cgcgctcgcc gaccgcggcg acctcgacgg cgtcgccagc gagcgccggc
     2101 ccgacctgat ggccaacgag atcgcggaga cctacacgtg cgcgagcgac ggcacgatct
     2161 cggtggtgat cgagaacatg tcgcgcggct acgcgcgcag ctacgagctc ggcgcgctgc
     2221 gctagcgccc gtcgcctgac gccgcccacg cggtccggta cggccgcacg aagttggcga
     2281 cgccggccgt gaccatgaac gcggccagcc agccaccggc gagcgtcatc cacgcgagct
     2341 gctcgacccg ggccgtcatg ctcgccatgt ccgccgggct gatccggccg atccgctccg
     2401 tcatgccctg tgcaacgcgc gaacagccga aacgattcgc accacccttc cccatggccg
     2461 ctgcatatat cacgtccggt cg
//
LOCUS       DASVOR010002418         3038 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_201936,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002418 DASVOR010000000
VERSION     DASVOR010002418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_201936"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VF516_44425"
     CDS             complement(<1..277)
                     /locus_tag="VF516_44425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protein kinase"
                     /protein_id="HEX8114881.1"
                     /translation="MSLVGQIAELQNLKTYKELSWEGSFEDYLELVRKNPQVTRNAYQ
                     RLYDMVLSHGVEEYIDNKKKLVRYKFFRDEAHGGRDAVFGLDVPLMRL"
     gene            complement(519..1607)
                     /gene="mltG"
                     /locus_tag="VF516_44430"
     CDS             complement(519..1607)
                     /gene="mltG"
                     /locus_tag="VF516_44430"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEX8114882.1"
                     /translation="MSRGSFRTALILVLGSLVVVGVVAGILLSRALAYPGEPHRGTGK
                     EVELEIRSGMSFPAISAMLASRGLIDRPSWFRLYAMWEGDTTNVKTGKYVLHDNLTPR
                     QVLAILVAGVKEVVTKVTLPEGKNMLEFFALIDAAKVAKAGELEALARDREFLGRHGI
                     VGDTVEGYLFPDTYEFRINEKPAVVLQRLITHHQEVWNDLLAKHPRDAAKIKDRLGWT
                     DRDVLIMASIVEKEAVDPAERPRIAQVFINRLTSPSFQPKRLETDPTIRYGCMVPVHK
                     SAACQQWDKRDRLHRAQLDDKDNPYNTYQHEGLPPGPICNPGKSSIQAVLSPDGSEYF
                     YFVAKDARNHAFARSFDEHKRNVEKYMK"
     gene            complement(1618..2088)
                     /gene="ruvX"
                     /locus_tag="VF516_44435"
     CDS             complement(1618..2088)
                     /gene="ruvX"
                     /locus_tag="VF516_44435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEX8114883.1"
                     /translation="MASEEICEIRQIRELPRALGLDVGSKTIGLALTDEAGIAAHPLT
                     VLSRVGNSGDARAVVAVITEHGVGDVVVGMPFELSGKRGHRARRVLEFVAALRAVLPA
                     GVRLHEQDERFTTAEAERALIAADLPRARRRSVIDRHAAALILQGWLDAQRRRG"
     gene            2677..2964
                     /locus_tag="VF516_44440"
     CDS             2677..2964
                     /locus_tag="VF516_44440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HEX8114884.1"
                     /translation="MSDEPSTPKLQVQIDEEIAQGVYSNLVLLNHTENEFVLDFAFIQ
                     PSNGRAKVRSRVISSPRHTKRLLQALQKNLERYEERFGAIDISSDDDPVFH"
BASE COUNT          480 a         1030 c         1010 g          518 t
ORIGIN      
        1 tcagccgcat cagcggcacg tcgagcccga acaccgcgtc gcgcccgccg tgcgcctcgt
       61 cgcggaagaa cttgtagcgg accagcttct tcttgttgtc gatgtactcc tcgacgccgt
      121 gcgagagcac catgtcgtac aaccgctggt acgcgttgcg cgtgacctgc gggttcttgc
      181 ggacgagctc gaggtagtcc tcgaagctgc cttcccagga cagctccttg taggtcttga
      241 ggttctgaag ctccgcaatc tgtcccacga gactcatggg atcgaccgta ccacacgccc
      301 actccgccgt ctgggttgcc gtcggcgttg tgcgcgacgg gcgcgtgagg ccgccgcgag
      361 ccgccccacc ctacttcatg tacttctcca cgttgcgctt gtgctcgtcg atcgagaggg
      421 ggccttccca tgaaccccct ctggggaatg cgccggcaaa gccggcgcat tcacccctcc
      481 gcgcgtcgcg tcgcgcggtt gttcatcggc cgctctcctc acttcatgta cttctccacg
      541 ttgcgcttgt gctcgtcgaa gctgcgagca aacgcgtggt tgcgggcgtc cttggcgacg
      601 aagtaaaagt actcgctccc gtcgggggac agcacggcct ggatcgagct cttgccgggg
      661 ttgcagatcg ggcccggcgg caggccctcg tgctggtagg tgttgtacgg gttgtccttg
      721 tcgtcgagct gggcgcggtg caaccggtcg cgcttgtccc attgctggca cgcggcagac
      781 ttgtggaccg gcaccatgca gccgtagcgg atcgtcggat cggtctcgag ccgcttgggc
      841 tggaagctcg gcgaggtcag ccggttgatg aacacctggg cgatccgcgg ccgctccgcc
      901 gggtccaccg cctccttctc gacgatcgac gccatgatca gcacgtcgcg atcggtccag
      961 ccgagccggt ccttgatctt cgccgcgtcg cgcgggtgct tggcgagcag gtcgttccag
     1021 acctcctggt ggtgggtgat cagccgctgc agcacgaccg ccggcttctc gttgatccgg
     1081 aactcgtagg tgtcggggaa caggtagccc tcgacggtgt cgccgacgat gccgtggcgg
     1141 ccgaggaact cccggtcgcg cgccagcgcc tcgagctcgc cggccttggc gaccttggcg
     1201 gcgtcgatca gggcgaagaa ctccagcatg ttcttgccct cgggcagcgt caccttggtc
     1261 acgacctcct tgaccccggc gaccaggatc gcgagcacct gcctgggcgt gaggttgtcg
     1321 tggaggacgt acttgccggt cttgacgttc gtggtgtcgc cctcccacat cgcgtacagc
     1381 cggaaccagc tcggcctgtc gatcagaccg cggctggcca gcatcgccga gatcgcgggg
     1441 aagctcatcc cgctccggat ctcgagctcg acctccttgc cggtcccgcg gtgcggttca
     1501 ccggggtagg ccagcgcccg gctgagcagg atccccgcga cgacgccgac cacgacgagc
     1561 gagccgagca cgaggatcag cgcggttctg aacgagcccc tggacactcc ggtgacacta
     1621 accgcgccgg cgctgcgcgt cgagccagcc ctgaagaatg agcgccgcgg cgtgccggtc
     1681 gatcacactg cgccgccgcg cacgcggcag atctgcggcg atcagcgcgc gctcggcctc
     1741 ggcggtggtg aaccgctcgt cctgctcgtg cagccgcacg ccggcgggga gcacggcgcg
     1801 cagcgccgcc acgaactcca gcacgcggcg cgcgcgatgg ccgcgctttc ccgacagctc
     1861 gaacggcatc ccgaccacga cgtcgcccac gccatgctcg gtgatcaccg cgaccacggc
     1921 gcgcgcatcg ccagaattgc cgacgcgcga gagcacggtc agggggtgcg ccgcgatccc
     1981 cgcctcgtcc gtcagggcca gcccgatcgt cttgctcccg acgtcgaggc cgagcgcgcg
     2041 cggcaactcg cggatttggc ggatttcgca gatctcttcg gaggccatgg cggttgccga
     2101 aaaaataaag gccctcctgg ggagggccaa ggagggccgg gcgcagatcg cagagctact
     2161 tgttgcctca gatagtgggg gagaccagcg ggatgagggg gaggggaacc gccgatcatc
     2221 tatcgagtcg aaactttgga cagctgcgtc gcgcgtgtaa acctttcgtt gttgccgaac
     2281 ccgacagccg ggttcatgcc ggggcatttc cgtgagccgt tggctcgttg acccaacacg
     2341 gagcagaatc cgggccagct cgtggctgag ctaccatcga gatctggccg acgagaaaat
     2401 cagcggttac gcacgcgatg tggtcggaaa accgacgccg ggtgccggtg ggccggcacc
     2461 tgctgtcacc gcgtgttctc aggctgtcca gtacagtccc aaccccggcg gaggacgttg
     2521 acgaagcttc gtacgcggag cggccgatgc cgcaggcgtc gcacgtcccg tgagccgcgg
     2581 atgatccggc attgccccgg ccggcattgc ccggggagaa ttctgccccg gccatgaccg
     2641 ggcggcgctg gcctgatgcg atacggtgca tccaccatgt cggacgagcc cagcacaccg
     2701 aagctccagg tgcagatcga cgaggagatc gcccagggcg tgtactcgaa cctggtgctg
     2761 ctcaatcaca cggagaacga gttcgtcctc gacttcgcgt tcatccagcc gagcaacggc
     2821 cgcgcgaagg tccgatcgcg cgtgatctcg tcgccccgcc acaccaagcg cctgctccag
     2881 gcgctgcaga agaacctcga gcgctacgag gagcggttcg gcgcgatcga catcagctcg
     2941 gacgacgatc cggtgttcca ttgacgggtc aggcgaccgc ggcgtccacg atcgccagcg
     3001 cgccgcggtc gaggtcgagc tcgcagcgcg cgccgaac
//
LOCUS       DASVOR010002419         5597 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_522841,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002419 DASVOR010000000
VERSION     DASVOR010002419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5597
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522841"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /gene="hisH"
                     /locus_tag="VF516_44445"
     CDS             complement(<1..402)
                     /gene="hisH"
                     /locus_tag="VF516_44445"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEX8114885.1"
                     /translation="MIAIVDVCSGNLRSVQRALTRAGGDVTVTRDAQAVRRADKLVVP
                     GQGAFGMFMRGLVERGLGDAIREAIASGRPFLGICLGMQVLFESSEEQGPCDGLGVIP
                     GRVVRIAPAAPHIKVPHIGWNRVVHHRPDPML"
     gene            complement(399..998)
                     /gene="hisB"
                     /locus_tag="VF516_44450"
     CDS             complement(399..998)
                     /gene="hisB"
                     /locus_tag="VF516_44450"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEX8114886.1"
                     /translation="MARTASIERKTKETQISLDLEVDGSGRHAMTTPVPFLSHMLDAF
                     ARHGLFDLSVRAAGDVEIDAHHTVEDIGLVLGDAFVRALGDRAGIVRYGSATLPMDDV
                     LVTVAVDFCGRPAFVWHVGNLDGKWIGDFDCELAKEFFAAFATRAQCNLHVVLHYGGN
                     AHHTIECIFKAFARACDAATRIDPRLGGAVPSTKGTLTT"
     gene            1073..2362
                     /locus_tag="VF516_44455"
     CDS             1073..2362
                     /locus_tag="VF516_44455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114887.1"
                     /translation="MVPLLWLGCGGGAKPPAAPKPEPAAVKPPPPPPETEEDRERKRH
                     AEALAIVPDKSSCLPAELRSPTAPRLELAAIGSAAVVCAIDQDRTRLLGPVACWAVAV
                     SGPNPGALTYQDAAPLPGRGLSVMLDDRCARGFCLPKEAKVPTDPVALITWNLENTKV
                     AVLVGDDLHIYNAQTKAHETSFSIRGDKGVTGDPTEIDWNGEAIFIQATDGTASPVFV
                     FKPDGIPVGPIEALGGGKDKAVLSTRNGSFLLLKPEQVAISEQGLTTLTIYETATGKR
                     TKLVRKLPASTCKKEELDAVWHDPTAQVSAKCRDYVTKNYAHLIGADAVAGTKNLLML
                     LRGPRLGELAVVDAKTLAEHKAIRMPWCDETGGGAPGGGGAPGAAAPAPSAAAGAPVG
                     ADKASSFAPAPAAEATAPDAKKAKGKSKTGDPDAGGQ"
     gene            complement(2760..3104)
                     /locus_tag="VF516_44460"
                     /pseudo
     CDS             complement(2760..3104)
                     /locus_tag="VF516_44460"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3555..4607)
                     /locus_tag="VF516_44465"
     CDS             complement(3555..4607)
                     /locus_tag="VF516_44465"
                     /inference="COORDINATES: protein motif:HMM:NF014021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEX8114888.1"
                     /translation="MCGTIPGHRNHGRDPDPILLAVAHGKPSVGVSRSHVRRFLHRAR
                     WPRRPSGLAARRTLTRHDMLVPVTELPVPPRVLATAGRLAEIPGVVGVVLGGSRAIGT
                     HRPDSDVDLGLYYRGPLDVAALRALADEAADQVLGMSEVGGWGPWVNGGAWLVVDGTR
                     VDWIYRDLDRVAAIWSECRAGRYEIGIQAGHPLGYYSHVYPGEVALCQVLADPTGELT
                     RLRAETAHYPPALAAALVAAVWEPDLLLYGASSYGAASADAFFVAGCLFRAIGVLAQA
                     LHVHHRRWVTTEKRLIDAAGRLAAAPPDFARRAHAILGSLGTTPDEIAATLAAARALV
                     DDTTARLPAKPAALPL"
     gene            complement(4741..>5597)
                     /locus_tag="VF516_44470"
     CDS             complement(4741..>5597)
                     /locus_tag="VF516_44470"
                     /inference="COORDINATES: protein motif:HMM:NF012242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEX8114889.1"
                     /translation="STARGLGAVSPGEPSPATTARGFQAPQSYPILPPPVPMAPPPPP
                     APQGSPLPYSPSVPVARPYSPPASPPPPPPYPPPAVSPAYVSPSPSLSPSPPYSAPVP
                     AAPSPSPSVPPPVAQAMYVPSPSAPTYVSPPMPAYAPPAPVAPPPAPVLLEVTPRGLG
                     IATVAGFCEELIRRNSRLPTEACKLFITSRDNQDQVRIVVCQGESRRLDNNTVIGDLV
                     LQGLPPRPRGETSIEVTFALDGSGVLQVRARDAQTGAEQRATLSLVGGVAQEDVAASR
                     DRLQQLRR"
BASE COUNT          854 a         1954 c         2058 g          731 t
ORIGIN      
        1 gagcatcggg tcggggcggt ggtggacgac ccggttccag ccgatgtgcg ggaccttgat
       61 gtgcggcgcg gcgggcgcga tccgcaccac gcggccgggg atcacgccga ggccgtcgca
      121 cgggccctgc tcctcgctcg attcgaacag gacctgcatg ccgaggcaga tcccgaggaa
      181 cggccggccg ctcgcgatcg cctcgcggat cgcgtcgccc aggccgcgct cgaccaggcc
      241 gcgcatgaac atgccgaacg cgccctggcc gggcacgacc agcttgtccg cgcgccggac
      301 ggcctgcgcg tcgcgggtga cggtgacgtc gccgccggcc ctggtcagcg cccgctggac
      361 cgagcgcagg ttgcccgagc acacgtcgac gatggcgatc acgtggtcag cgtccccttg
      421 gtcgacggca cggcgccgcc gagccgcggg tcgatccggg tcgcggcgtc gcacgcccgc
      481 gcgaacgcct tgaagatgca ctcgatggtg tggtgcgcgt tgccgccgta gtgcagcacg
      541 acgtgcaggt tgcactgcgc gcgggtcgcg aacgcggcga agaactcctt ggcgagctcg
      601 cagtcgaagt cgccgatcca cttgccgtcg aggttgccga cgtgccacac gaacgccggg
      661 cggccgcaga agtcgaccgc gacggtgacc agcacgtcgt ccatcggcag cgtcgccgag
      721 ccgtagcgca cgatgccggc gcggtcgccg agcgcgcgga cgaacgcatc gcccagcacc
      781 aggccgatgt cctcgacggt gtggtgggcg tcgatctcga cgtcgccggc cgcgcgcacc
      841 gagagatcga acaggccgtg gcgcgcgaac gcgtcgagca tgtgcgacag gaacggcacc
      901 ggcgtggtca tcgcatgccg gccgctcccg tcgacctcga ggtccaggct gatctgcgtc
      961 tccttggtct tgcgctcgat ggaagcagtg cgcgccacgg ctccttgtat caggtggtcc
     1021 gcgatcgtac aaacgtccta tggtctggca accagcgaca atcgctcgcg ttgtggtccc
     1081 gctcctgtgg ctggggtgcg gcggtggcgc aaaaccgccc gccgcgccca agcccgagcc
     1141 cgcggcggtc aagccgcccc ccccgccgcc ggagaccgag gaggaccgcg agcgcaagcg
     1201 ccacgccgag gcgctcgcga tcgtccccga caagagctcg tgcctgcccg ccgagctccg
     1261 gagcccgacc gcgccgcggc tcgagctggc cgcgatcggc agcgccgcgg tggtgtgcgc
     1321 gatcgaccag gatcgcaccc ggctgctcgg gccggtcgcg tgctgggccg tcgcggtcag
     1381 cggtcccaat cccggcgcgc tgacctacca ggacgcggcg ccgctgcccg ggcgcggcct
     1441 ctcggtgatg ctcgacgacc gctgcgcccg cgggttctgc ctgcccaagg aggccaaggt
     1501 ccccaccgac ccggtcgcgc tgatcacgtg gaacctggag aacaccaagg tcgcggtgct
     1561 ggtcggcgac gatctccaca tctacaacgc ccagaccaag gcgcacgaga ccagcttctc
     1621 gatccgcggc gacaagggcg tgaccggcga tccgaccgag atcgactgga acggcgaggc
     1681 gatcttcatc caggccaccg acggaacggc gtccccggtg ttcgtgttca agcccgacgg
     1741 catcccggtc ggtccgatcg aggcgctcgg cggcggcaag gacaaggcgg tgctgtcgac
     1801 ccgcaacggg tccttcctgc tgctcaagcc cgagcaggtc gcgatctccg agcaggggct
     1861 caccacgctc acgatctacg agaccgcgac cggcaagcgc accaagctgg tgcgcaagct
     1921 cccggcatcg acctgcaaga aggaggagct cgatgcggtg tggcacgatc cgaccgccca
     1981 ggtctcggcc aagtgccggg actacgtgac caagaactac gcgcacctga tcggtgccga
     2041 cgcagtcgct ggcaccaaga acctgttgat gctgctgcgc ggcccgcggc tcggcgagct
     2101 cgcggtggtc gacgccaaga cgctcgccga gcacaaggcg atccggatgc cgtggtgcga
     2161 cgagaccggc ggtggcgcgc ccggcggtgg tggcgcaccc ggcgccgccg ccccagcacc
     2221 cagcgccgcg gccggcgctc cggtcggcgc agacaaggcg agcagcttcg cgccggcacc
     2281 tgcggccgag gccactgcgc cagacgcgaa gaaggcgaag ggcaaatcga agaccggcga
     2341 ccccgacgcc ggcggccagt agagcgcgcg cctgtagcgc atagcgcccg ggagaacgct
     2401 gacacgcgac tcgccgcggg ctgctgcgcg gatgggcgtc gtggccgcgc ccgccgaatc
     2461 accacccgtc ctgcgcggac ccctctaggt atgtgcggga caatgcccgg ccaatccaga
     2521 cctgaaactt acccggcagc gacctcgttc ttggtgaggg tgaagccaag ggatcgggcg
     2581 gcttcggaga tcccggcgag gcggcgagcc tggaacttgg cctcgaacgc cgcctcgccg
     2641 atgtccacgt agtcttggcc gtagcgcagc atccggtaga tcagccgggc gagttgacga
     2701 gcggtcgcga agaccgcaac ggcgacgccc ttgtgacgag cgatacgccg aaacgaggcc
     2761 ttgcgtcccg ggacgttgtg aagctgccgg gcgttggcga gcacgacctc gagcccgcgc
     2821 gattcgagca gctcgaacac cgggatccag tagacggacg tgctctccat ggcgaccgac
     2881 gtcacgcctt gcgtgacgag ccagtctgcg agctcgcgga gctgcggggt cgtggtgccg
     2941 aacgcgcgga cgttgggtgc gccgtcggcc cgagcaggtc cgcagaccca gtgctgcgtg
     3001 ctgccgacgt ccacgccagc gaccatcggt tgaaggaccg gaagcgccgc cggtcctgcc
     3061 ttcgtcgtcg tcttcttctt gccgtctcgc ttgacatccg acatgttcac ctcgtgaggc
     3121 gaacggtccc ggcccgagct ggcgattgtg tggaaccaga cctcggggag atcctggtcg
     3181 cttcctcgac caagcctcgc caatgaacga tggccatctc ggacccaaac aaatcacggg
     3241 gcatgagctc caatgattgg cggggcgtag ctgccgggac ctcacctctc cccttcgatc
     3301 gcagatcccc ctcaagtttc atcctggatg atccgccgtc cacggcggtg gtagaacaaa
     3361 tcacggtcgc gaccccgacc ccatcctgct cgcggtcgcc cacggcgagc caagcgttgg
     3421 cgcctggcga tcgcgggaag accggccggc cgtattcgtc cgagcagtcc tgcttgctgc
     3481 cgccgtcgtc gcgcgagtgg ctgccggccg ggcatctggc gtacttcgtc ctcgggcggt
     3541 tggacgagct cgacctagag cggcagcgct gccggcttgg ccggtagccg cgcggtcgtg
     3601 tcgtccacca gcgcccgggc ggcagcgagc gtggccgcga tctcgtcggg cgtggtgccg
     3661 aggctgccga ggatcgcatg ggcgcggcgc gcgaagtccg gcggcgcagc ggcgagccgg
     3721 ccggcggcgt cgatcagccg cttctcggtg gtcacccagc ggcggtggtg gacgtgcaac
     3781 gcctgggcga gcacgccgat cgcgcggaac aggcagccgg cgacgaagaa cgcgtcggcc
     3841 gacgccgcgc cgtagctgga cgcaccgtag agcaacaggt ccggctccca gaccgcagcg
     3901 accagcgcag cggccagcgc cggcgggtag tgcgcggtct cggcgcgcag ccgggtgagc
     3961 tcgccggtcg gatccgcgag cacctggcag agcgcgacct cgccggggta gacgtgcgag
     4021 tagtagccga gcgggtgccc ggcctggatg ccgatctcgt agcggccggc gcggcactcg
     4081 gaccagatcg cggcgacgcg gtcgaggtcg cggtagatcc agtcgacccg tgtgccgtcg
     4141 acgaccagcc aggccccgcc gttgacccac gggccccagc cgccgacctc ggacatcccg
     4201 agcacctggt cggcggcctc gtcggcgagc gcgcgcagcg cggcgacgtc gagcggcccg
     4261 cggtagtaca gcccgaggtc gacgtccgag tcgggccggt gcgtgccgat cgcgcggctg
     4321 ccgccgagca ccacgcccac cacacccggg atctcggcga gccggccggc cgtggccaga
     4381 acgcgcgggg gcacgggtag ctcggtcacg ggcacgagca tgtcatggcg cgtgagcgtc
     4441 cgtctggccg cgagcccgga agggcgtcga ggccagcggg cccgatggag aaaccttcga
     4501 acgtgcgatc gcgagacgcc aacgctcggc ttgccgtggg cgaccgcgag caggatgggg
     4561 tcggggtcgc gaccgtgatt acgatggccg ggtattgtcc cgcacatacc tcgcggggcc
     4621 cgcgcaagac gggtggcgat tcggcgggcg ctgccacgac gcccatccgc gcagcagccc
     4681 acggcgagcc gagcgtgctc gcgttctccc ggcgctacgc cccaccatgc acctgactag
     4741 ctagcgccgg agctgctgga ggcgatcgcg cgatgccgcg acgtcctcct gggcgacgcc
     4801 gccgaccagc gagagggtcg cgcgctgctc cgcgccggtc tgggcgtcgc gtgcccggac
     4861 ctgcagcaca ccggacccgt cgagcgcgaa cgtgacctcg atcgaggtct cgccacgcgg
     4921 tcggggcggc agaccctgca gcacgaggtc gccgatcacg gtgttgttgt cgagccggcg
     4981 cgactcgccc tggcagacga cgatccgcac ctggtcctgg ttgtcgcgtg aggtgatgaa
     5041 cagcttgcag gcctcggtcg gcagccgcga gttgcggcgg atcagctcct cgcagaaccc
     5101 ggcgaccgtg gcgatgccga ggccgcgcgg cgtgacctcg agcagcacgg gcgccggtgg
     5161 cggcgcgacc ggggccggag gtgcatacgc cggcatgggc ggagacacgt acgtgggcgc
     5221 ggacggagaa ggtacgtaca tcgcttgcgc cacgggcgga ggcacggacg gcgagggcga
     5281 tggggcagcc ggcacgggcg ccgagtacgg cggtgatggc gacagggatg gggatggcga
     5341 cacgtaggcc ggagacaccg ccggcggtgg atacggcggt ggtggcggcg gtgatgccgg
     5401 cggcgagtac ggccgggcaa ccggcacgga cggcgagtac ggcagtggcg atccctgcgg
     5461 cgccggcggg ggtggcggcg ccatcggcac cgggggcggc aggatcggat agctctgcgg
     5521 cgcctggaag ccccgggcgg tggtcgcggg cgacggctcg ccgggcgaga ccgcgccgag
     5581 gccacgagcc gtggacc
//
LOCUS       DASVOR010002420         3357 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_85176,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002420 DASVOR010000000
VERSION     DASVOR010002420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85176"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1295)
                     /locus_tag="VF516_44475"
     CDS             complement(24..1295)
                     /locus_tag="VF516_44475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114890.1"
                     /translation="MTRTQLDTPFLDGGIRSTNFFNGRLLSREDMQREQDAERAVHER
                     LGRALGAGIAYGLEVEAKAIGGNSAADPVVTVHAGLAINRRGQTVALDRDVDVSLLKP
                     AAPATSTTSAAGAFATCLPPDNAVYVTGTGVYLLAVAPASQKQGLAVVSGLNNTAAAC
                     NAKEVVAGVQFHLTQVKLTSAELADDAHLRNAVAYKFFAAEGAGTDAVRDPFGVPPSP
                     VALTDPPLSDCDVPLGVFQWTAAGGLRWVDLWSVRRRPAGPDSDAWPMFPGARPDAVG
                     EAMLRQFQHHIGSLRDASALPTRASDVFRSLPPAGLVPLGGIAGAVGVDAARFFDGFT
                     TRGPVFVEGARVPAFLRLATRFPPIRVGDPELVWLYLVRENRQPVAGVVPRQPYLIFA
                     LGRLPYQAAPRFDVSHWDFANYALDDERMSP"
     gene            complement(1309..2760)
                     /locus_tag="VF516_44480"
     CDS             complement(1309..2760)
                     /locus_tag="VF516_44480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114891.1"
                     /translation="MSGFGPSPVYPGALALDPTKHVNYVEGMVLGSADLRQEFAYHAQ
                     RPQWLVRDLVGYGTVWGLAVSQRTGARGPEVVVSSGVALDPRGQFIRVAPAQCAPLND
                     WLAGRTADILARKQTTANPNVSALTLYVVLGYRSCDSDPVPIAGEPCRTEQDSVAPSR
                     TGDDFQLELAFDPPPQPEEDALREFFAWLQPHITVTGGGAASLTIAQFLDEIRAAARA
                     AAAAVTSPPGAGPRLVDKSPPMHLSVPAALLPDYVRATLRLWATELRPLWRPDWLGDK
                     HGWAGDEPFASADNGNQVVLAKLTLRIEPPGLNSTAWTVARDVDVSPPDAPIAIADDV
                     RPYVLPLRFLQEWLITQSGAAGGGGGGGPSAGPQVVAAGVLNANATANARSAFNGLKV
                     QSVTSTATATQLLLSFGGYSVPPTTGGPQYLVKVLPSQTAIPNLVITFDSFQAGGIQL
                     LLTKTVPPATTPIALVTADLNALQLMVEVSRYP"
     gene            complement(2757..>3357)
                     /locus_tag="VF516_44485"
     CDS             complement(2757..>3357)
                     /locus_tag="VF516_44485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114892.1"
                     /translation="QAFVAQALGFVPDASATAGRAWQQFLQRRYRRIAAAAAAHATPW
                     ASFADVRLPDTLPADGAPLLDWYAFQSLGLGLRRTAHRFTVLLPVVPSEAFAVDTLLA
                     RRDLARRIVEIEKPAHTLFDVKLYWAMFRIGEARLGSDTLLHIGSRAPELMPSLVLGQ
                     GFLAESYLAASPPESVPERPIVGRDPLGAQRCHIEETTP"
BASE COUNT          468 a         1101 c         1352 g          436 t
ORIGIN      
        1 atcgacattg cgtcctcctg cgctcacggg ctcatgcgct cgtcgtcgag cgcgtagttg
       61 gcaaagtccc agtgcgacac gtcgaagcgc ggcgccgcct ggtacggcag gcggccgagc
      121 gcgaagatca ggtacggctg cctgggcacg acgccggcga ccggctggcg gttctcgcgc
      181 accagataga gccacaccag ctcgggatcg ccgacgcgga tcggcgggaa ccgggtggcc
      241 aggcgcagga acgcggggac gcgcgcgccc tcgacgaaca ccggcccgcg cgtggtgaag
      301 ccgtcgaaga atcgggcggc atcgaccccg accgccccgg cgatcccgcc gagcgggacg
      361 aggccggccg gcggcagcga gcggaacacg tcgctcgcgc gggtcggcag cgcgctggca
      421 tcgcgcaggc tgccaatgtg gtgctggaac tggcgcagca tggcctcgcc gaccgcgtct
      481 ggccgcgcgc cggggaacat cggccaggcg tcggagtccg gccctgccgg ccgccggcgc
      541 accgaccaca gatcgaccca gcgcaggccg ccggcggcgg tccactggaa caccccgagc
      601 gggacgtcac agtcggacag cggcggatcg gtgagcgcga ccggcgaggg cgggacgccg
      661 aacgggtcgc gcacggcgtc ggtgccggcg ccctcggcgg cgaagaactt gtaggcgacg
      721 gcgttgcgca ggtgggcgtc gtcggcgagc tcggcggagg tcagcttgac ctgggtgagg
      781 tggaactgga cgcccgcgac gacctccttg gcgttgcacg cggcggcggt gttgttgagg
      841 ccgctgacca cggcgaggcc ctgcttctgc gatgcgggcg cgacggccag caggtagacg
      901 ccggtgccgg tgacgtacac cgcgttgtcg ggcggaaggc aggtggcgaa cgcgccggcg
      961 gccgaggtcg tggacgtcgc cggggcggcg ggcttgagca gcgagacgtc gacgtcgcgg
     1021 tcgagcgcga cggtctggcc gcggcggttg atcgcgaggc cggcgtgcac ggtgacgacc
     1081 gggtcggcgg cggagttgcc gccgatcgcc ttggcctcga cctcgaggcc gtacgcgatg
     1141 ccggcaccca gggcgcggcc gaggcgctcg tggacggcgc gctcggcgtc ctgctcgcgc
     1201 tgcatgtcct cgcgcgacag gaggcgcccg ttgaagaagt tggtggagcg gatgccgccg
     1261 tcgaggaacg gggtatcgag ctgggtccgg gtcatcgtcg atctccggtc atgggtaccg
     1321 gctcacttcc accatcagct gcagcgcatt gaggtcggcg gtgacgagcg cgatgggggt
     1381 cgtggcgggg ggaacggtct tggtcagcag gagctggatg ccgccggcct ggaagctgtc
     1441 aaaggtgatc accaggttgg gaatggcggt ctggctcggc agcaccttga ccaggtactg
     1501 cggccctccg gtggtgggcg gcacgctgta gccgccgaac gacagcagga gctgcgtcgc
     1561 cgtggcggtg ctggtgacgg attgcacctt cagcccgttg aatgccgagc gcgcgttcgc
     1621 cgtggcgttg gcgttgagca cgcctgccgc gacgacctgg ggccccgcgg acggtccgcc
     1681 accgccgcca ccaccggcgg ctccgctctg cgtgatcagc cactcctgga ggaagcgcag
     1741 cggcagcacg taggggcgca cgtcatcggc gatcgcgatc ggcgcgtcgg gcggcgagac
     1801 gtcgacgtcg cgcgccacgg tccaggccgt gctgttcagg cccggcggct cgatcctgag
     1861 cgtcagcttc gcgaggacga cctggttgcc gttgtcggcg gaggcgaacg gctcgtcgcc
     1921 ggcccagccg tgcttgtcgc ccagccagtc ggggcgccac agcgggcgta gctcggttgc
     1981 ccagaggcgc agggtggcgc gcacgtagtc gggcaagagc gcggcgggga ccgacaggtg
     2041 catcgggggc gacttgtcga cgagccgtgg gccggcgccg ggcggcgagg tcactgccgc
     2101 agcggccgcg cgggccgcgg cgcggatctc gtcgaggaac tgcgcgatgg tgagcgatgc
     2161 cgcaccgccg ccggtcaccg tgatgtgggg ctgaagccag gcgaagaatt cgcgcagcgc
     2221 gtcctcctcg ggctgcggcg gcgggtcgaa cgcgagctcg agctggaagt cgtcgccggt
     2281 gcgcgacggc gcgaccgagt cctgctcggt gcggcacggc tcgccggcga tcgggaccgg
     2341 atcgctgtcg cagctgcggt agccgagcac gacgtagagc gtcagcgcgg acacgttcgg
     2401 gttggcggtg gtctgcttgc gcgcgaggat gtcggcggtt cgcccggcga gccagtcgtt
     2461 gagcggcgcg cactgcgccg gcgcgacccg gatgaactgg ccgcgcgggt cgagcgcgac
     2521 gccggagctg accacgacct cgggaccgcg cgcgccggtg cgctgcgaga ccgcgagccc
     2581 ccacacggtg ccgtagccga cgaggtcgcg gaccagccac tgggggcgct gcgcgtggta
     2641 ggcgaactcc tggcgcagat cggcggagcc gaggaccatg ccctcgacgt agttgacgtg
     2701 cttggtcgga tcgagcgcca gtgcgcccgg gtagaccgga ctcggaccga agccactcat
     2761 ggggtcgtct cctcgatgtg acagcgctgc gccccgagcg gatcgcgtcc gacgatcggg
     2821 cgctcgggaa cactctcggg gggtgatgcg gcgaggtagc tctcggcgag gaagccctgg
     2881 ccgagcacga gcgatggcat cagctcgggc gcgcggctgc cgatgtggag cagcgtgtcg
     2941 ctgccgagcc gggcctcgcc gatccggaac atcgcccagt acagcttgac gtcgaacagg
     3001 gtgtgggccg gcttctcgat ctcgacgatc cggcgggcca gatcgcggcg ggcgagcagg
     3061 gtgtcgaccg cgaacgcctc gctcgggacc accgggagca gcacggtgaa gcggtgcgcg
     3121 gtgcggcgca gcccgaggcc cagcgactgg aacgcgtacc agtcgagcag cggggcgccg
     3181 tcggcgggca gggtgtccgg cagccggacg tcggcgaagc tggcccaggg cgtggcgtgg
     3241 gcggcggccg ccgccgcgat ccggcggtag cggcgctgca ggaactgctg ccaggcgcgc
     3301 ccggcggtcg cgctggcgtc ggggacgaag ccgagcgcct gggcgacgaa cgcctgc
//
LOCUS       DASVOR010002421         1610 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_172639,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002421 DASVOR010000000
VERSION     DASVOR010002421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1610
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172639"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VF516_44490"
     CDS             <1..181
                     /locus_tag="VF516_44490"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEX8114893.1"
                     /translation="ELRPIEADILRQRFGLGTEQELTLKEIGDKYNLSRERIRQLQEQ
                     ALAKMRRALARRDLM"
     gene            363..782
                     /locus_tag="VF516_44495"
     CDS             363..782
                     /locus_tag="VF516_44495"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HEX8114894.1"
                     /translation="MRRSPHRFSAVVLLGLLGSLGLGAACGNDNPKLLVTGIEPEKGA
                     DGSYVRIHGNRFTADGPRSVRVLFGDQQAQVDRFESDSELIVTAPGGNKVGDVVNVTI
                     LFEPGGKLTLPKAFRYVEKAEGPSVNDLNTGPNRPKK"
     gene            complement(816..>1610)
                     /locus_tag="VF516_44500"
     CDS             complement(816..>1610)
                     /locus_tag="VF516_44500"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8114895.1"
                     /translation="TPGFPPPAATGMRRCPSCGSDVPQTFRFCGTCGFRMDEASAPVG
                     MQQPGLGAPVARTRLSMTLIRPDGTEGGSHDLRPGENKLGRSFGAVFENDGYLSPVHA
                     QLDIRGGTAVIRDLDSLNGVFVKMTQEEELTNGQIIRIGQELLRFEVIAAPEPTADGT
                     ELMGSPNPGYWGKLTVIIGRDITGAAFPLLGDSCTLGRERGDINFPDDGYVSGLHARV
                     ALRDGRVFLADLGSSNGTFIKVNGERPIGHESFVLLGQQLFRLNLA"
BASE COUNT          245 a          540 c          594 g          231 t
ORIGIN      
        1 cgagctgcgg ccgatcgagg ccgacatcct gcgccagcgc ttcgggctgg ggaccgagca
       61 ggagctgacg ctcaaggaga tcggcgacaa gtacaacctg tcgcgcgagc ggatccgcca
      121 gctccaggag caggcgctcg cgaagatgcg acgtgcgctg gcgcgccgcg atctcatgta
      181 gtggaagaag aaccgcgcga cgcgacgcgc ggaggggtga atggccgggc tttgcccggc
      241 cattcggcgg gaaagcccct ctcgatcgat gcggcgcgcg ctggcgcgcc ggacctgatg
      301 taggtagttg taggcgacac gggagttgcc cgagggtatt cgggttgggt acgtttcctg
      361 tcatgaggcg ttcgccgcat cggttttcgg ccgtcgtcct gctcgggctg ctcgggtcgc
      421 tcggcctggg cgcagcctgc ggcaacgaca accccaagct cctcgtcacc gggatcgagc
      481 ccgagaaggg cgcggacggc agctacgtcc ggatccacgg caaccggttc accgccgacg
      541 ggccgcgcag cgtgcgggtc ctgttcggcg accagcaggc gcaggtcgat cggttcgaga
      601 gcgacagcga gctgatcgtc accgcgccgg gcggaaacaa ggtcggcgac gtggtcaacg
      661 tgacgatcct gttcgagccg ggcggcaagc tgacgctgcc caaggcgttc cgctacgtcg
      721 agaaggccga ggggccgtcg gtcaacgacc tgaacaccgg ccccaaccgg cccaagaagt
      781 agcgccgcgc gccctgcgcg cgccctgcgc gagccctacg cgagattgag ccggaacagc
      841 tgctggccga gcagcacgaa cgactcgtgg ccgatcgggc gctcgccgtt gaccttgatg
      901 aacgtgccgt tcgagctgcc gaggtcggcg aggaacaccc ggccgtcgcg cagcgcgacc
      961 cgggcgtgca ggccggacac gtagccgtcg tcggggaagt tgatgtcgcc gcgctcgcgg
     1021 ccgagcgtgc agctgtcgcc gagcagcggg aacgccgcgc cggtgatgtc gcggccgatg
     1081 atcacggtca gcttgcccca gtagcccggg ttcgggctgc ccatcagctc ggtgccgtcg
     1141 gcggtcggct cgggcgcggc gatcacctcg aagcggagca gctcctggcc gatccggatg
     1201 atctggccat tggtcagctc ctcctcctgc gtcatcttga cgaacacgcc gttgaggctg
     1261 tcgagatcgc ggatcaccgc ggtgccgccg cggatgtcga gctgggcgtg gaccggcgac
     1321 aggtagccgt cgttctcgaa caccgccccg aagctgcggc cgagcttgtt ctcgccgggg
     1381 cgcaggtcgt ggctgccgcc ctcggtgccg tccggccgga tcagcgtcat cgacagccgg
     1441 gtgcgcgcga ccggggcgcc gagccctggc tgctgcatcc ccaccggggc cgacgcctcg
     1501 tccatgcgga agccgcaggt gccgcagaac cggaaggtct gcgggacgtc gctgccgcac
     1561 gacggacagc gccgcatgcc ggtcgcggcc ggcgggggga accccggggt
//
LOCUS       DASVOR010002422         2209 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_522893,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002422 DASVOR010000000
VERSION     DASVOR010002422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2209
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_522893"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..941)
                     /locus_tag="VF516_44505"
     CDS             complement(<1..941)
                     /locus_tag="VF516_44505"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HEX8114896.1"
                     /translation="MATTAQRPLDAHLDSNLGAVAELAAQLRVDSIRSSTSAGSGHPT
                     SSMSAADLLAVLLTRHLRYDWDHPGSPTNDHLILSKGHASPLLYAVFKAAGVVSEQEL
                     MTGYRRFHQRLEGHPTPVLPWVDVATGSLGQGLPDGVGVALAGKYLERQPYRVWVLCG
                     DSELAEGSVWEAFDKASYYQLSNLIVIVDVNRLGQRGETELGWHLDVYARRVEAFGAR
                     TLVINGHDLSSIDGALAIAGDTSGDRPTVIVAKTIKGRGFPEVEDKLDWHGKPFPRDM
                     AERAIAELGGVRNTVVRSPRPPAVSPGAGTPAAPAARP"
     gene            1679..1948
                     /locus_tag="VF516_44510"
     CDS             1679..1948
                     /locus_tag="VF516_44510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114897.1"
                     /translation="MAKIELPEGWARVADPLRALMAEIERESHADGSAPPDLTAVSAR
                     WAQVSDAIRTTLRGRIASRRAQAADGPMREHPSDSGSGGGSGEAS"
BASE COUNT          361 a          687 c          811 g          350 t
ORIGIN      
        1 ggcctggcgg cgggcgccgc gggtgtgccg gcaccgggag agaccgccgg ggggcgcggg
       61 ctgcgcacga ccgtgttgcg aacgccgccg agctcggcga tcgcgcgctc ggccatgtcc
      121 ctgggaaacg gcttgccgtg ccagtcgagc ttgtcctcga cctccgggaa cccccggccc
      181 ttgatcgtct tggccacgat cacggtgggg cgatctccgg acgtgtcgcc ggcgatcgcc
      241 agggcgccgt cgatcgaaga caggtcgtga ccgttgatga ccagcgtgcg tgcgccgaac
      301 gcctcgacgc ggcgggcgta gacgtcgagg tgccagccga gctcggtctc gccgcgctgg
      361 ccgagccggt tgacgtcgac gatcacgatc aggttcgaca gctggtagta cgacgccttg
      421 tcgaacgcct cccagaccga gccctcggcg agctcgctgt cgccgcacag cacccagacc
      481 cggtacggct gccgctcgag gtacttgccg gcgagcgcga cgccgacgcc gtcgggcagt
      541 ccctggccga gcgatccggt cgcgacgtcg acccacggca gcaccggcgt cgggtgcccc
      601 tccagccgct gatggaaccg gcgatagccg gtcatcagct cctgctcgga gaccacgccg
      661 gcggccttga acaccgcgta cagcagcggg gaggcgtggc ccttggagag gatcaggtga
      721 tcgttggtgg gcgatccggg atggtcccag tcgtagcgca gatggcgggt gagcagcacg
      781 gccagcagat cggccgcgga catgctggac gtcgggtggc ccgagccggc gctggtggac
      841 gagcggatcg aatcgacgcg cagctgggcc gcgagctcgg cgacggcacc gaggttggaa
      901 tcgaggtggg cgtcgagagg acgttgggcg gtcgtggcca tgggggaagt gtgggaacgg
      961 ttgccgccgc cgacgagccg cgtgtccgcc gcacacccgc gcacgtgcgg cagagcctca
     1021 ccgtgcgcgc cggacccgcg atcgggaatc gcctggcgag cgttgcgagc tgcgaagctg
     1081 gacatctggg atacccaggg atcgcggatc gtcatcgtcg gacgagagag cggcgatcac
     1141 ccggcggtgg agcaggttcg cgccaagaca atcgatgtgc tccgcccgcc cggcgcgtgg
     1201 ctactctcga caccggccgg acgtccgcgg cccgcgcgtt caacggcgcg ccgcgcggac
     1261 gcggtgagat tcgcagccaa ggagctttac gataggactt acctgaccgc ccaaaacggt
     1321 agggttggtt ttgggtttcc gctaaccccg cggtcacgat cgcgcccgcg ttcagcaaca
     1381 cgttcgccgg gttcctggtc gcgcaggccc tcggggcagc cgccggggcg tgtgtgttct
     1441 cgtggctggt gccagcacag cccagcgacg cgccctcgac cgggtgatac cgcggactgg
     1501 tccgcggtat ctgatggaat cctgatttaa ctccgctaga tataaatcgc aggggtgtgg
     1561 atctaatact gtgacgtccg aagtgaggta acgcgcggat ctgcaagcag aaagcgcgaa
     1621 gccgggcccc tgaaaatcgg aatttgccgc gggatcgggg gcggggcacg atggggccgt
     1681 ggccaagatc gaacttcccg agggctgggc ccgcgtggcg gatccattgc gcgcgctgat
     1741 ggccgagatc gagcgcgaat cgcatgcgga cggctcggcg cctccagatc tcacggcggt
     1801 gtcggcgcgg tgggctcagg tgagcgacgc gatccgtaca actctgcgag gacggattgc
     1861 tagtagacga gcacaagccg cagatgggcc gatgcgcgag cacccaagtg atagcggatc
     1921 aggtggcggc agtggcgagg cttcctaacg gggccgtcct ggtcaagatc cgctttgacg
     1981 gcatcgttca ccacgccgtt gtgatccgcg tgcatggggg gcacctgggt ggactcgcct
     2041 gcgaggccgt gctcaatgac gatgaatgct tgtcgaagag cggacgagag ttagtgccag
     2101 accctgtcga ttgcatgtca tgcctcgtcg gtatcgcacg ttgggatcgg taagaggatc
     2161 gttgattgcg caggcactcg cgccgaagcg acatgtcctg cgagtttgg
//
LOCUS       DASVOR010002423         3750 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_494437,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002423 DASVOR010000000
VERSION     DASVOR010002423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494437"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="VF516_44515"
     CDS             <1..811
                     /locus_tag="VF516_44515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114898.1"
                     /translation="GRLCCVKWDRDSCFLGRLRQRLEAIQRGRTARQLVVDQKAQKQE
                     LRPIDFPLTGLPPRDGLRGHVEGCCDLGRLHSQGLPHELEGLAAPEPTLHEFRRHEEL
                     EPDGGRTVHRRVAAYRASPARQLSSKLDTGNRDGVRNRAWDKIRLATMDAFHESPPWR
                     HSEQRDDEHLVIDDRDERQIVAIEIAADELPATVILHPTLRRVQERMLDVRKGNATLL
                     HSGLPVILIYLPSVRSREGANQLKHLSLGHVHLGSDLTKRQAALDDPSYTC"
     gene            complement(869..2503)
                     /locus_tag="VF516_44520"
     CDS             complement(869..2503)
                     /locus_tag="VF516_44520"
                     /inference="COORDINATES: protein motif:HMM:NF018471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HEX8114899.1"
                     /translation="MADAEDTRPDALPVEPREGVGEGGGGGLRATDRLRGALEAVRRR
                     LGPPRSYVITRWLILRLLGVVYLFAFLGIVWQGLPLLGEHGLTPAGAYVEQLRDAGQS
                     FWDVPSLFLLDPSDAALRGWAIAGAVLSAALVLGYANLPSLAALWLIYGSYERIGQLW
                     WGFGWEIQLLETTVIAACLVHPWDPRPLRAPAPPRTAIVLMRWLAFRIMLGAGLIKLR
                     GDPCWTALTCLDAHFETQPIPNPLSPLFHHLPHAVHAAGVVFNHVAEVVLPWFAFGPR
                     RLRLVAALGMLGFQGVLILSGNLAFLNWLTVVPVLALLDDDFVVRCVPRRWRDRLRGA
                     STAPRPPRLGHRIFVACIAALVAIKSLPVVANLASHHQAMNRSYDRLALVNTYGAFGS
                     VGMERFELVIEGTRDPDPATARWSAYELPCKPGALDRRPCVLGPYHRRLDWLIWFAAM
                     SDRPTTDSVWVIHLVWKLLDGDRGIRTLLGPDPFHGAPPRFVRIRRFRYHLEPSGGAT
                     WWTRDDEQLWLRPVALDTPGLRETLEQFGMPSPTLR"
     gene            complement(2597..3523)
                     /locus_tag="VF516_44525"
     CDS             complement(2597..3523)
                     /locus_tag="VF516_44525"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEX8114900.1"
                     /translation="MLVERGHTVRGLARTRSAILADLGVEHVRGDVLAGPELDRALDG
                     VEAVCHLAGAVSRDPGDAQRMMRLHVDGTRRVLERMAAAGVPRMVLASTSGTIGVSKD
                     EVILDETAPYAEDIVAGWPYYASKIYQERLAFEHGKRLGIAVVAVNPSLLLGPGDRRL
                     SSTGDVRRFLRREIPTIPDGGVSFVDARDAAEATAAALERGRAGERYLLGGPNWTTRE
                     FFARLGRIANIAPPRLKLPASWARWGAGVMEELYRWRGKEPPVDRISVEMAEHYWWID
                     SSKAERELGFAARDPQLTLVDTVAYLRQGVDD"
BASE COUNT          624 a         1358 c         1273 g          495 t
ORIGIN      
        1 ggggcgatta tgctgcgtga aatgggatcg tgactcttgc ttcctgggcc ggctccgcca
       61 gcgcctggag gcgatccaac gtggtcgtac ggcccgccag cttgtcgtcg accagaaagc
      121 ccagaagcag gaactccggc caatcgactt ccctctgacc ggtctcccac cgagagacgg
      181 tctccggggc cacgttgaag gctgctgcga tctcggtcgc cttcattccc agggccttcc
      241 gcatgaactt gaaggactcg ccgctccgga gcccacgctt cacgagttcc gccgccacga
      301 ggagctcgaa ccggacggcg gcaggaccgt tcaccgtcga gtagccgcat accgggcatc
      361 gccggcccgg cagctcagca gcaaactcga caccggcaac cgtgacggcg tgcggaaccg
      421 cgcctgggac aagatccgcc ttgccacaat ggatgcattt catgaatctc ctccatggag
      481 gcactcagaa caacgtgatg acgagcacct cgtcatcgac gaccgcgatg agcgtcagat
      541 cgtcgccatc gagatcgcag ctgacgagct tccagcgacc gttatcttgc acccgacact
      601 gcgtcgcgtt caggagcgca tgctcgacgt ccgcaaagga aacgccacgc tgctgcattc
      661 gggactgccc gtgatactga tctacctccc atcggttcgc tctcgtgagg gagcgaacca
      721 gctcaagcat ctgtcgctcg gtcatgttca ccttggatct gacttgacga aacgtcaagc
      781 agcacttgac gatccgtcct atacctgctg acagcttagc aaaaatcaca ggttagcgat
      841 gcctgaccag gctgcgactt acctcccatc accgcagcgt cggggacggc atgccgaact
      901 gctcgagcgt ctcgcgcagg ccgggggtgt cgagcgcgac cgggcgcagc cacagctgct
      961 cgtcgtcgcg ggtccaccag gtcgcgccgc cggacggctc gaggtggtag cggaaccggc
     1021 ggatccggac gaagcgcggc ggtgccccat gaaatggatc tgggccgagc agcgtgcgga
     1081 tcccgcgatc gccgtcgagc agcttccaca ccaggtggat cacccacacc gagtcggtgg
     1141 tcggccggtc ggacatcgcc gcgaaccaga tcagccagtc gagccggcgg tggtacgggc
     1201 cgagcacgca gggccgccgg tcgagcgcgc cgggcttgca cggcagctcg taggccgacc
     1261 accgcgcggt ggcaggatcg gggtcgcgcg tgccctcgat cacgagctcg aagcgctcca
     1321 tgccgaccga gccgaacgcg ccgtaggtgt tgaccagcgc gagccggtcg tagctccggt
     1381 tcatcgcctg gtggtgcgac gccaggttcg cgacgaccgg gaggctcttg atcgcgacca
     1441 gcgcggcgat gcaggcgacg aagatccggt ggccgaggcg gggcgggcgc ggcgccgtgc
     1501 tcgccccgcg cagccggtcg cgccatcgcc gcggcacgca gcgcacgacg aagtcgtcgt
     1561 cgagtaacgc gagcaccggc acgacggtca gccagttgag gaacgcgagg ttgccgctga
     1621 ggatcagcac gccctggaag ccgagcatgc cgagcgcggc gaccagccgc agccgccgcg
     1681 gaccgaacgc gaaccacggc agcacgacct cggcgacgtg gttgaacacc acgccggccg
     1741 cgtggacggc gtgcggcagg tggtgaaaca gcggcgacag cgggttgggg atcggctggg
     1801 tctcgaagtg cgcatcgagg caggtcagcg cggtccagca cggatcgccg cgcagcttga
     1861 tcaacccggc cccgagcatg atgcggaacg ccagccagcg catcagcacg atcgcggtgc
     1921 gcggcggcgc cggcgcgcgc agcggccgcg gatcccaggg atgcaccagg caggccgcga
     1981 tcaccgtggt ctccagcagc tggatctccc agccaaaccc ccaccacagc tggccgatcc
     2041 gctcgtacga gccgtagatc agccacagcg ccgccagcga cggcaggttg gcgtagccga
     2101 gcaccagcgc cgccgacagc acggcccccg cgatcgccca gccgcgcagc gccgcatcgg
     2161 agggatcgag caggaacagg ctcggcacgt cccagaacga ttgccccgcg tcgcgcagct
     2221 gctcgacgta cgcgcccgcc ggcgtcagcc cgtgctcgcc gagcagcggc aggccctgcc
     2281 acacgatgcc gaggaacgcg aacaggtaca ccacgccgag cagccggagg atcagccagc
     2341 gcgtgatcac gtagctgcgc ggggggccga gccggcggcg cactgcctcg agcgcgccgc
     2401 gcagccgatc ggtcgcgcgc aggcccccgc ccccgccctc gcccaccccc tcccgcggct
     2461 cgacgggcaa cgcgtcgggg cgagtgtcct cggcgtcggc catgacggcc gcagggaacc
     2521 ctgctcgatc gcgcacaagt cgagctcgcc caacggcccg aggatgaagt accgggtgct
     2581 atgcgctaca ggttccctag tcgtccacgc cctggcgcag gtacgcgacc gtgtcgacca
     2641 gcgtcagctg gggatcgcgc gcggcgaacc cgagctcgcg ctcggccttc gacgagtcga
     2701 tccaccagta gtgctcggcc atctcgaccg agatccgatc gaccggcggc tccttgccgc
     2761 gccagcggta gagctcctcc atcacgccgg ccccccaccg cgcccacgac gccggcagct
     2821 tgagccgcgg cggcgcgatg ttcgcgatcc ggccgagccg cgcgaagaac tccctggtcg
     2881 tccagttcgg cccgccgagc aggtagcgct cgcccgcccg gccgcgctcc agcgccgcgg
     2941 cggtggcctc ggccgcgtcg cgcgcgtcaa cgaagctgac cccgccgtcg gggatcgtcg
     3001 ggatctcgcg gcgcagaaac cggcggacgt cgccggtcga cgacagccgg cgatcgcccg
     3061 gcccgagcag cagcgacggg ttgaccgcga ccacggcgat gccaagccgc ttgccgtgct
     3121 cgaacgcgag ccgctcctgg tagatcttcg aggcgtagta cggccagccc gcgacgatgt
     3181 cctcggcgta cggcgcggtc tcgtcgagga tcacctcgtc cttcgacacg ccgatcgtcc
     3241 cgctggtcga tgccagcacc atgcgcggca caccggccgc ggccatccgc tccagcacgc
     3301 gccgcgtgcc gtcgacgtgc agccgcatca tccgctgcgc gtcgccggga tcgcgcgaca
     3361 ccgcgcccgc gaggtggcac accgcctcga cgccgtcgag cgcacggtcg agctcgggac
     3421 cggccaggac gtcgccgcgg acgtgctcga cgccgaggtc ggcgaggatc gccgagcgcg
     3481 tgcgggccag gccgcgcacg gtgtggccgc gctcgaccag cacgcggcac caggtgatac
     3541 atcgacgcgt cgctcgcgaa ggcgacgcgg tcgaactccc ccgccagcac ccgccccggc
     3601 gcggtggccc gctccaactc gtcgacgagc gggtcgcggg ccaccggcgt caggcgctct
     3661 tgagcttggt ggcgtagtcg cggcggagct tcgcgacctt gggcgccacc tggaagaggc
     3721 agtagggctg cagcgggttc cgctggtaat
//
LOCUS       DASVOR010002424         4428 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_85254,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002424 DASVOR010000000
VERSION     DASVOR010002424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4428
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85254"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VF516_44530"
     CDS             complement(<1..486)
                     /locus_tag="VF516_44530"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8114901.1"
                     /translation="MGAAIAPLPAGLLRMIGPGTPLEPEIRQAMEGVFEADFSTVRVH
                     HGSAAPALGAQAFTIGDSLYFAPGLYDPTSRGGVVLLGHELTHVVQQRAGRVANPYGH
                     GVALVQDPVLEAEADRMGRFVAEELWSDSGKPRSPVTRRPGPRTPARIQLSARLAGWT
                     CG"
     gene            complement(1349..1495)
                     /locus_tag="VF516_44535"
     CDS             complement(1349..1495)
                     /locus_tag="VF516_44535"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8114902.1"
                     /translation="MLDALEFLHGHGIVHRDLKPSNILVDLEGIVRVLDFGLARSVVT
                     RTTR"
     gene            2187..4061
                     /locus_tag="VF516_44540"
     CDS             2187..4061
                     /locus_tag="VF516_44540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114903.1"
                     /translation="MISGSGIDAGIGSDGSGSSIGSDAGGIGSDAGGIGSDAGGIGSD
                     AGGIGSDAGGGDGSGSCTTSLAPEPPRPPDPDPQPDPGPAPDQCTYDTCQALASGQLG
                     TSGVSQQTLDQCNGVSPAPSSCVFTPYPSFYLGENGCSVSEPSAGDLGNFRFDCPTDP
                     YEQQALCEFALATLQPVPPQIDPTPWEPGVGSGSGSGSGSVSDDFQNPNPGNANRNVV
                     VIGSSMLATHNQVNNGPQCARFDINGRVLTAADDRNLVGNDFTASPAWSTRVPWLRVF
                     RGARTGHKSGDVRSGTMNTPTPDTCGQNWNQNVSPMTAGVNWFAGKSNRLMITDGGLI
                     NDQKNVAGQSWTDTLASIVTCQALDRATGLINTLLDWDHWVQNWGWAGWLENRRWVWR
                     QAPTVRLQRTSPVGGWAQALDAPANMRRPRCQFVVQIRGNGRMPAALPAVLAIGKDVT
                     PLERLWRNGPAGSPVPGNLTAIKQAYVGVSKQAWLQYPYMDSAQVAIRGSDLGLLWNG
                     GAAGALVRAWGRNIALRINAIDPGIAPRIRQVTDDLNELMYISIGCNGAAAFAANHPA
                     RVCQRDAAMNPIVVITQSNFAGWGAGDHQDTIIGGMPHESAAGANKLGAALVAADALP
                     "
     gene            complement(4058..>4428)
                     /locus_tag="VF516_44545"
     CDS             complement(4058..>4428)
                     /locus_tag="VF516_44545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114904.1"
                     /translation="ASAPAAPAERPGKVALGPVRLLTSEAEFAQRTSAEQLGAFCREL
                     QRCVERVLADRDEPLELRLQVMCRPRSHRVGLSHKGDASEDLLDALIDALEQIPTLPV
                     RAEVSFELELLVGVGPRERF"
BASE COUNT          719 a         1565 c         1517 g          627 t
ORIGIN      
        1 gccgcaggtc cacccggcaa ggcgtgcaga gagctggatc cgtgccggtg tcctcgggcc
       61 cgggcggcga gtcaccggcg atcggggctt ccccgagtcc gaccacagtt cctcggcgac
      121 gaatcgcccc atccgatccg cctccgcctc cagcaccgga tcctgcacga gcgccactcc
      181 atggccatag ggattcgcga cccggccggc gcgctgctgc accacatggg tcagctcgtg
      241 cccgagcagc acgaccccgc cgcgactcgt cggatcgtag agcccgggcg cgaagtacag
      301 actgtccccg atggtgaacg cctgcgctcc cagcgccggc gccgccgacc catggtgaac
      361 gcgtaccgtg gaaaaatcgg cctcgaaaac accctccatc gcctgccgga tctcgggctc
      421 cagcggtgtg ccggggccaa tcatccgcag caatccggcc ggcagcgggg cgatcgcagc
      481 ccccacgcgc gaccgcggct gcgcggcccc accgagcatc ccgacccgca atgggcccat
      541 cccgggcaat cgctccggcc ggaccgccgc gggccagcca gcgttccggc ccgatccgga
      601 cccggcccgc actgcctcgc cccctgtccc ggaaggcatg agctcgggac gtggtgctct
      661 cgcagtccac gggtcctgga gtgcgagcgg aaccttcgcg agggctctcc cgtcgccgcg
      721 aaaccgctcg atgtggaagt acccctgcag caccatgccg cgtgtcggtc ggctcagtgc
      781 catcgccgct ctcgcgatgg cgggggagcg accgccgatc tcgtctcggt cacgggcagg
      841 cacctgcttc gcccgcggcg gatcggccgg cagcagcacg ccggcgcccc ggcagctccc
      901 cgccggccgc cgtgagacaa ggcgcgaccc gatgcctgca tggtgaccga tcgcttcagc
      961 gacgagggtt gcgtgtcgac gaaggcatgg ccgtgagtgg catgatacgc accgcagggt
     1021 gcggggcaaa aataagacca ggccagcgcc ccgagtccgc ccggagcagc tcgctgggcg
     1081 acggcggcgg ttccacgatg accagcggcg acgcctcgcg tcaaggcaag atcgtcggtc
     1141 gtcgccggct ggccgagctc gccgggcctc gtgatctgtc cagctgaacg agccgcttcg
     1201 gaggaggcag gaccatgtct gacgtttcgt cgaccccggc ggcaccgggg cagctcaccg
     1261 gagccagcgt ccggttccac aggatctttg tccgcgacaa ccacgcaggg ctcggagggc
     1321 cggggaagat cgacatcggc agtgacatct atctcgtcgt cctggtgacg acagatctcg
     1381 ccagcccgaa gtcgagcacg cgcacgatgc cctcgaggtc gaccaggatg ttcgacggtt
     1441 tgaggtcgcg atgcacgatg ccgtggccgt gaaggaactc cagggcatcg aggatcgtgg
     1501 ctcatcggcg caccgcgcct ggaacgccgg tcgctccgag cgagatggca gggcaggcag
     1561 gaagcacgcg gcgactgtac tggttcacgg ctatcctgcg cgctcgggcc gcagcgcaat
     1621 ccgtgcgccg caatgcccgg tccgcacgca ccgcgcccga catcaagatt ccgcgcagca
     1681 gctgcgcgtc ccggtgcgcc gcgcgcagca cgatgcgcga tcggggtcca cgcgatcaac
     1741 gtcacacgcc ggttcgtgcg cgatcgccgt cgtctgcgtc aggcctgcga ggttttggcg
     1801 tgcagcctcc acgagccctg gtgcccagcg gatcgtcgct ccggcctctc gtgagcgagt
     1861 ccccgtcggg cctgccggaa cggctcgaac aacatgcgga gcggccgccg caccgcgcta
     1921 cagcgcatac gcacagccga tcagggtgag gtgggctgtg gtgctacgcc gctcatcaac
     1981 gctaagtcac catcaacgct gagcgccgat ccgccgcgga ggcactgcgt gcgctggcac
     2041 actcgatgct ttggccccga gcactacctt ggtcggaggt cgacatgcca aagatgcgtc
     2101 cacgttctca tccattcgct gtggtgctgc tcgtcgccgc gggttgcacg ggcaacctcg
     2161 gcgaagcgca atcgtcgagc cccagtgtga tatcgggcag cggcatcgac gccggcatcg
     2221 gcagcgacgg cagcggcagt agcatcggta gcgacgcggg cggcatcggt agcgacgcgg
     2281 gtggcatcgg cagcgacgcg ggcggcatcg gcagcgacgc gggtggcatc ggcagcgacg
     2341 cgggtggcgg cgacggcagc ggcagttgca ccaccagtct cgcgccggaa ccgccgcgac
     2401 cgccggatcc ggacccgcag ccggatccgg gacccgcacc ggatcaatgc acctacgaca
     2461 cctgccaggc gctcgcgtcg ggtcagctcg gtacaagcgg cgtcagtcag cagacgctcg
     2521 accagtgcaa cggcgtgtcg ccggcaccct cgtcgtgcgt gttcacgccg tatcccagct
     2581 tctatctcgg tgagaacggc tgcagcgtat ccgagccttc ggccggcgac ctcggcaact
     2641 tccggttcga ctgcccgacg gacccctacg agcagcaggc gctgtgcgag tttgcgcttg
     2701 cgacgctgca gccggtgccg ccgcagatcg acccgacgcc ctgggagccc ggcgtgggct
     2761 cgggttccgg ttccggctcg ggcagcgtat ccgatgactt ccagaacccg aatccgggca
     2821 acgcaaatcg caacgtcgtc gtgatcggct cgtccatgct ggcgacccac aaccaggtca
     2881 ataacggccc gcagtgcgcc cggtttgaca tcaatggacg ggtgttgacc gcagccgacg
     2941 accgcaatct cgtcggcaat gacttcaccg cgtcccctgc gtggagcacg cgcgtcccgt
     3001 ggctgcgcgt gttccgcggt gcgcgtaccg ggcacaagag cggcgacgtc cgcagcggga
     3061 cgatgaacac gccgacgccc gatacctgcg gccagaactg gaaccagaac gtgtcgccga
     3121 tgacggccgg cgtcaactgg tttgccggca agagtaaccg gctgatgatc accgacggtg
     3181 ggctgatcaa tgatcagaag aacgtcgccg gccagtcatg gaccgacacg ctggcgagca
     3241 tcgtgacctg ccaggcgctc gaccgggcga ccggactcat caacacgctc ctcgactggg
     3301 atcactgggt ccagaactgg ggctgggcgg gatggctcga gaacaggcgc tgggtctggc
     3361 ggcaggcgcc gacggttcgc ctgcagcgca cgtcgccggt cggaggctgg gcccaggcgc
     3421 tcgacgctcc ggcgaacatg cgccggccgc ggtgccagtt cgtggtccag atccggggca
     3481 acggcaggat gccggccgcg cttcccgcgg tcctcgcgat cggcaaggac gtgaccccgc
     3541 tcgagcggct atggcgcaac gggccggcag gaagcccggt gcccggtaac ttgactgcga
     3601 tcaagcaggc gtatgtcggc gtgtcgaagc aggcctggct gcaatacccg tacatggact
     3661 cggcgcaggt cgcgattcgc ggttctgacc tcggtctgtt gtggaacggt ggagccgcgg
     3721 gggcgctggt ccgcgcatgg ggacgcaata tcgcgctcag gatcaatgcg atcgatccgg
     3781 gcatcgcgcc gcgcatccgc caggtcaccg acgatctcaa cgagctgatg tacatctcga
     3841 tcggctgcaa cggcgccgcg gcgttcgcgg ccaatcaccc ggcgcgcgtg tgccagcgcg
     3901 acgccgcgat gaacccgatc gtcgtgatca cgcagagcaa cttcgcgggt tggggtgcgg
     3961 gcgatcacca ggacacgatc atcggcggca tgccccacga gagcgccgcg ggagcgaaca
     4021 agctgggcgc cgcgctcgtc gccgccgatg cgctgcccta gaaccgctcg cgcggcccga
     4081 cgcccaccag cagctccagc tcgaagctga cctcggcgcg gaccggcagc gtcgggatct
     4141 gctcgagcgc atcgatcagc gcgtccagca gatcctccga ggcgtccccc ttgtgggaca
     4201 ggccgacgcg gtggctgcgc ggccggcaca tcacctggag ccggagctcg agcggctcgt
     4261 cgcgatcggc gagcacgcgc tcgacgcagc gctgcagctc gcggcagaac gcgccgagct
     4321 gctcggccga cgtccgctgg gcgaattcgg cctccgacgt caacagccgc accgggccga
     4381 gcgcgacctt gccggggcgc tccgccggcg ctgccggcgc ggaggcct
//
LOCUS       DASVOR010002425         6003 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_172695,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002425 DASVOR010000000
VERSION     DASVOR010002425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172695"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..656
                     /locus_tag="VF516_44550"
     CDS             <1..656
                     /locus_tag="VF516_44550"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEX8114905.1"
                     /translation="LRRAANDGEYFWSYEVAWHQSQLSDLISGELWAHCQGELPETVV
                     ARNRRRFDASGHKKRDYLDYMIYAMMQDNYFGNLMLSKLDLLSSRLGLEPRCPFTEPA
                     YAHWVYNVPSSFKYRDNWVKYFFKKSIEGVLPHDIIYRPKQGFRTPAQELFAGRLGTW
                     ARPVLLETGFTRLGLLRRDALAALLDAHNRRERDNSTKLWTAMVLNLWHTRYIENKG"
     gene            659..2230
                     /locus_tag="VF516_44555"
     CDS             659..2230
                     /locus_tag="VF516_44555"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX8114906.1"
                     /translation="MRAAVVVLACLGFVSLGGCVIYLNPLCNDQIVNGDETGIDCGGS
                     CPGKCEVGGGCRTDADCDDAICMGGVCTAKPCDNGKQDGQETDVDCGGGGGAGLACRA
                     CAGGRKCLVDADCFSPGGCNPVTKTCSALTVSFAPEVRYFSGYKAYALLSGDLDGDGH
                     ADLAVINEYGSSVAVFLDNVTTGAFTRLANPAGKPPDEPDNRALNFGPTGAYPTGGGV
                     ADFNHDGKLDVVTADYHGYSVTVLLNDGTGKLKTQAAPYPTEAKAETSNLAVGDLNKD
                     GNPDVVATNPQTASVSVFLSHADGTLDPAINLQVGVMNGAQPYSAVIADFDGDGNNDL
                     AVAEEMSGQIFVRLGHGDGTFGDETGYAIDGTRDYILIAHDMNNDKKLDLVCANRNGD
                     TVSVLLGRGDGTFRKAIVSSVGPPDVMKMAGRPWFGPYNVAVADVNQDGVPDVVTPNF
                     VGDSVSILLGIGDGHFAPAIELKYDKPTDPMFTSTTPYGIVVGDFNGDGKPDIATANA
                     GSDDLTIRLNTGQKN"
     gene            2264..3802
                     /locus_tag="VF516_44560"
     CDS             2264..3802
                     /locus_tag="VF516_44560"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX8114907.1"
                     /translation="MAVVGLCAALTGCVRYLDPQCNDQIRNGDETGIDCGGGCGRCPV
                     GGGCRADTDCEDATCVAGVCTALPCENGVRDGRETDVDCGGGTCHACAGGRACRVADD
                     CGSGACDPITMTCAALSVSFAPEDRYFSGFKSYALLPADLDGDGATDLAVINEYGSSV
                     AVFRNDYATGGAFTRVPNPGPRPPDEPDNRALNFGPTGAFPTGGGIADVNHDGKPDVV
                     TANFHGSSVTVLLDDGTGRLNLKPPASYPTEAKAETSNLAIGDLDKDGNPDVVATNPQ
                     TASVSVFLGHADGTLDPAIRLPVGTSPGAQPFSAAIADFDGDGHNDLAVAEETTGSLL
                     VRLGHGDATFGPEVAYAIGGVRDYIVIARDMNLDGKLDLVCAARNGDTVSVLLGRGDG
                     TFRKAIVSSVVPPGVMLAQDRPWLGPYAIAVADINRDGIPDVVTPNFVGDSVSILLGI
                     GNGHFDPAIELKFGRPADPSFSTTTPYGIVAADFNGDGKPDIATANAGSDDLAVRLNT
                     GQLH"
     gene            complement(3813..5348)
                     /locus_tag="VF516_44565"
     CDS             complement(3813..5348)
                     /locus_tag="VF516_44565"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEX8114908.1"
                     /translation="MPEPSTADSSAAPASASASLTARTVRGAAWTLSTSVSTRVIGLV
                     GSLLMARYLDKGEYGEVTAAMILTLTAFTVTSLGVGNYLLSNPGLGRAEVFHATCWFL
                     STGVAVLAVAWAISGPIGPWFDAPHLSRYMPIYVAAALCDRIEFLPERMLIRKLRFRW
                     LSIARAAGELTYTGLSLGLLWAGVGAIALAWAYLARSALKLVAIAFAVDWREWAEPHR
                     LRMATLRAIIRYGSGVSLASIAAALIRRWDNLLVSRYFGAPAMGAYNYAYNLADTPAT
                     AIGEPLTDVVTAAFPHAEGDKRQAALTRACTMISLIMFPLAFGLGAVADTVVEAFLPP
                     EWANVGPMLMYLSILAAPRPLASIVQAYLFAGHHVRVIIWLEWGSFLTLMAAMAAFGS
                     LAAGSPMFGSSAILWTCGVVGTVFVLRTLASFATVTWLDGVPVRRFLAPLVRPLIACA
                     LMVAAILAVRPALDGHRPVVRLGIEIALGAAVYLAGAVGIFRGAAAEFVDLLRSSFLR
                     RRR"
     gene            5507..>6003
                     /locus_tag="VF516_44570"
     CDS             5507..>6003
                     /locus_tag="VF516_44570"
                     /inference="COORDINATES: protein motif:HMM:NF015666.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HEX8114909.1"
                     /translation="MKIAVIGSGYVGLVAGAGFSDFGNHVTCVDIDPSRIERLRRGEI
                     PFHEPGLSDLVQRNARQGRLVFTTDTAEAVRDTEVVFLAVGTPSLPDGSADLRYVLEA
                     ARQVGRGLTGYTVIVNKSTVPVGTGDLVKKTVAEVTTQPFTVASNPEFLKEGDAINDF
                     LKPARV"
BASE COUNT          997 a         2140 c         2087 g          779 t
ORIGIN      
        1 tgctgcgccg cgccgccaac gacggcgagt acttctggag ctacgaggtc gcgtggcatc
       61 agagccagct gtccgacctg atctccggcg agctgtgggc gcactgccag ggcgagctgc
      121 ccgagacggt ggtcgcccgc aaccgccggc ggttcgacgc cagcgggcac aagaagcgcg
      181 actacctcga ctacatgatc tacgcgatga tgcaggacaa ctacttcggg aacctgatgc
      241 tctcgaagct cgacctgctg tcgtcgcggc tcggcctgga gccgcgttgc ccgttcaccg
      301 agccggcgta cgcgcactgg gtctacaacg tcccgtcgtc gttcaagtac cgggataact
      361 gggtcaagta cttcttcaag aagtcgatcg agggcgtctt gccccacgac atcatctacc
      421 gtcccaagca ggggttccgc accccggcgc aggagctgtt cgccgggcgg ctgggcacct
      481 gggcccgccc ggtgctgctc gagaccgggt tcacccggct cggacttttg cgccgcgacg
      541 cgctggccgc gctgctcgac gcacataacc gccgcgagcg tgacaacagc accaagctgt
      601 ggaccgcgat ggtgctgaac ctctggcaca cgaggtacat cgaaaacaaa ggatgagcat
      661 gcgcgctgcc gtcgtggtgc tggcctgcct ggggttcgtg agtctgggcg ggtgtgtgat
      721 ctacctgaac ccgctgtgca acgaccagat cgtgaacggc gacgagaccg gcatcgactg
      781 cggcggcagc tgccccggca agtgcgaggt cggcggtggg tgcaggaccg acgccgactg
      841 cgacgacgcc atctgcatgg gcggcgtgtg caccgcgaag ccgtgtgaca acggcaagca
      901 ggatgggcag gagaccgacg tcgactgcgg cggcgggggt ggtgcgggac tagcgtgtcg
      961 cgcgtgcgcc ggcggccgca agtgcctggt cgacgccgac tgcttcagcc ccggcggctg
     1021 caacccggtg acgaagacct gctcggcgct caccgtatcg ttcgccccgg aggtccgcta
     1081 cttctcgggc tacaaggcct acgcgctctt gtcgggcgac ctcgatggcg acggtcacgc
     1141 cgatctcgcg gtgatcaacg agtacggcag ctcggtcgcg gtgttcctcg ataacgtcac
     1201 caccggcgcg ttcacccggc tggccaaccc ggccggcaag ccgcccgacg agccggacaa
     1261 ccgggcgctc aacttcggcc cgaccggcgc gtatccgacc ggcggcggcg tggccgactt
     1321 caaccacgac ggcaagctcg acgtcgtcac cgccgattac cacggctact cggtcaccgt
     1381 cctgctcaac gacggcacgg gcaagctgaa gacccaggcc gccccctacc cgaccgaggc
     1441 caaggccgag acctcgaacc tcgcggtcgg ggacctcaac aaggacggca accccgacgt
     1501 ggtcgcgacc aacccgcaga ccgcctcggt cagcgtgttc ctgagccacg ccgacggcac
     1561 gctcgacccg gcgatcaacc tgcaggtcgg cgtgatgaac ggcgcgcagc cgtactccgc
     1621 ggtgatcgcc gacttcgacg gcgacggcaa caacgacctg gcggtcgccg aggagatgag
     1681 cgggcagatc ttcgtccggc tcggccacgg cgacgggacg ttcggcgacg agacgggcta
     1741 cgcgatcgac ggcacccgcg actacatcct catcgcccac gacatgaaca acgacaagaa
     1801 gctcgacctg gtgtgcgcca accgcaacgg cgacacggtc agcgtgctgc tcggccgcgg
     1861 cgacggaacg ttccgcaagg cgatcgtgtc gtcggtcgga ccgcccgacg tgatgaagat
     1921 ggcgggtcgg ccgtggttcg ggccgtacaa cgtcgcggtg gccgacgtca accaggacgg
     1981 cgtgcccgac gtcgtgacgc ccaacttcgt cggcgacagc gtctcgatcc tgctcgggat
     2041 cggcgacggc cacttcgctc cggcgatcga gctcaagtac gacaagccga ccgacccgat
     2101 gttcacgagc acgacgccgt acggcatcgt cgtcggcgac ttcaacggcg acggcaagcc
     2161 cgacatcgcc accgcgaacg ccggctcgga cgacctgacg atccggctga acacgggcca
     2221 gaagaactga cgaccccggg aggtggctat gacggttcgt gcggtggcag tggtggggct
     2281 gtgcgcggcg ctaaccgggt gcgtgcgcta cctggatccg cagtgcaacg accagatccg
     2341 caacggcgat gagaccggga tcgactgcgg cggcggctgc gggcggtgcc cggtcggcgg
     2401 cggctgccgg gcggacaccg actgcgagga cgccacctgc gtcgccggcg tctgcacggc
     2461 gctgccgtgc gagaacggcg tccgggacgg ccgggagacc gacgtcgatt gcggcggcgg
     2521 tacctgccac gcgtgcgccg gcggccgcgc gtgccgggtc gcggacgact gcggcagcgg
     2581 cgcctgcgat ccgatcacga tgacctgcgc ggcgctctcg gtgtcgttcg cccccgagga
     2641 ccgctacttc tccgggttca agtcgtatgc gctgctcccg gccgacctcg acggcgacgg
     2701 cgcgaccgac ctcgcggtga tcaacgagta cggcagctcg gtggcggtgt tccgcaacga
     2761 ctacgccacg ggcggcgcgt tcacccgggt gcccaacccg ggtccgaggc cgcccgacga
     2821 gccggacaac cgggcgctca acttcggtcc gaccggggcg ttcccgaccg gcggcgggat
     2881 cgccgatgtc aaccacgacg gcaagcccga cgtggtcacg gcgaacttcc acggcagctc
     2941 ggtcaccgtc ctgctcgacg acggcacggg ccggttgaac ctcaagccgc cggcgagcta
     3001 cccgaccgag gccaaggccg agacctcgaa cctcgcgatc ggggacctcg acaaggacgg
     3061 caaccccgac gtggtcgcga ccaacccgca gaccgcgtcg gtcagcgtgt tcctcggcca
     3121 cgccgacggc acgctcgatc cggcgatccg cctgccggtc gggacctcgc ccggcgcaca
     3181 gccgttctcg gcggcgatcg ccgacttcga cggcgacggc cacaacgacc tggcggtcgc
     3241 cgaggagacc accgggagcc tcctcgtgcg gctcggccac ggcgacgcga cgttcggccc
     3301 cgaggtggcc tatgcgatcg gcggcgtccg cgactacatc gtcatcgccc gcgacatgaa
     3361 cctcgacggc aagctcgacc tggtgtgcgc cgcccgcaac ggcgacaccg tcagcgtgct
     3421 gctcgggcgc ggcgacggga cgttccgcaa ggcgatcgtg tcgtcggtcg tcccgcccgg
     3481 ggtgatgctt gcacaggacc ggccgtggct cggcccgtac gcgatcgcgg tcgccgacat
     3541 caaccgggat ggcatcccgg acgtggtgac gcccaacttc gtgggcgaca gcgtctcgat
     3601 cctgctcggg atcggcaacg gccacttcga tccggcgatc gagctcaagt tcggccggcc
     3661 ggccgatccg tcgttcagca ccacgacgcc ctacggtatc gtcgccgccg acttcaacgg
     3721 cgacggcaag cccgacatcg ccaccgccaa cgccggctcg gacgacctcg cggtccggct
     3781 gaacaccggc cagctccact gaccggcggc ggtcaccgcc ggcgccgcag gaagctcgag
     3841 cgaagcaggt cgacgaactc ggccgcggcg ccgcggaaga tcccgaccgc gccggcgagg
     3901 tagaccgcgg cgccgagggc gatctcgatg ccgagccgga ccaccggccg gtgaccgtcc
     3961 agcgcgggtc ggaccgcgag gatcgccgcc accatgagcg cgcacgcgat cagcggccgg
     4021 accagcggcg cgaggaaccg gcgcaccggc acgccgtcga gccaggtcac ggtcgcgaag
     4081 ctcgccagcg tgcgcagcac gaacaccgta ccgaccacgc cacaggtcca caggatcgcg
     4141 ctcgacccga acatcgggct gcccgcggcc agcgagccga acgccgccat cgcggccatc
     4201 agggtgagga agctccccca ctcgagccag atgatcacgc ggacgtggtg accggcgaac
     4261 aggtacgcct ggacgatcga cgcgagcggc cggggcgccg ccaggatcga caggtacatc
     4321 agcatcgggc cgacgttggc ccactcgggg ggcaagaacg cctcgaccac ggtgtcggcg
     4381 accgcgccga gcccgaacgc gagcgggaac atgatcagcg agatcatcgt gcaggcgcgg
     4441 gtcagcgcgg cctggcgctt gtcgccctcg gcatggggga atgccgcggt cacgacgtcg
     4501 gtcagcggct cgccgatcgc cgtcgccggc gtgtcggcga ggttgtaggc gtaattgtag
     4561 gcgcccatcg ccggcgcgcc gaagtaccgg ctgaccagca ggttgtccca gcggcggatc
     4621 agcgcggcgg cgatcgaggc cagcgacacc cccgagccgt agcggatgat cgcgcgcagc
     4681 gtggccatgc gcagccggtg cggctccgcc cactcccgcc agtcgaccgc gaatgcgatc
     4741 gcgaccagct tgagcgccga gcgggccagg tacgcccacg cgagcgcgat cgcgccgacg
     4801 ccggcccaca acagaccgag cgacagcccg gtgtaggtca gctcgccggc ggcgcgcgcg
     4861 atcgacagcc agcggaaccg gagcttgcgg atcagcatcc gctccgggag gaactcgatg
     4921 cggtcgcaca gcgcggccgc gacgtagatc ggcatgtagc gcgacaggtg gggcgcgtcg
     4981 aaccacgggc cgatcgggcc gctgatcgcc caggccacgg cgagcacagc gacgccggtc
     5041 gagaggaacc agcacgtcgc gtggaacacc tcggcgcggc cgagcccggg gttcgatagc
     5101 aggtagttgc cgaccccgag cgacgtcacg gtgaacgcgg tcaacgtcaa gatcatcgcc
     5161 gcggtgacct cgccgtactc gcccttgtcg aggtagcgcg ccatcagcaa cgagcccacc
     5221 aggccgatca cgcgcgtgct gacgctggtc gacagggtcc acgccgcgcc ccggaccgtc
     5281 cgggcggtca ggctcgccga tgcggacgcc ggggcggcag acgagtcagc ggtcgacggc
     5341 tcgggcatgg ctggcgttcg accgcaggcg acggcccacc cactgtatca ccggtcgtcg
     5401 ccgggcacgc gggcctgaac cggctctcac gccgcccgcg cgtgcggcga cgcgcgcggc
     5461 aaaaccagtt gcgcgaggcc gcgccgactg gagatagtgg ggcgccatga agatcgcggt
     5521 catcggctcg ggatacgtcg gcctcgtcgc cggcgcgggg ttctcggatt tcggcaacca
     5581 cgtgacgtgc gtcgacatcg atccgtcgcg gatcgagcgg ctgcggcgcg gcgagatccc
     5641 gttccacgag cccggcctgt ccgacctcgt gcagcgcaac gcgcgccagg gccggctggt
     5701 gttcaccacc gacaccgccg aggcggtgcg cgacaccgag gtcgtgttcc tcgcggtcgg
     5761 cacgccgtcc ttgcccgacg ggtcggccga cctgcgctac gtgctcgagg ccgcgcgcca
     5821 ggtcggccgc gggctcaccg gctacaccgt gatcgtcaac aagagcacgg tcccggtcgg
     5881 caccggcgac ctggtgaaga agaccgtcgc cgaggtcacc acccagccgt tcacggtcgc
     5941 gtcgaacccc gagttcctca aggagggcga cgcgatcaac gacttcctca agccggcgcg
     6001 cgt
//
LOCUS       DASVOR010002426         1988 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_494489,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002426 DASVOR010000000
VERSION     DASVOR010002426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1988
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494489"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VF516_44575"
     CDS             complement(<1..751)
                     /locus_tag="VF516_44575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114910.1"
                     /translation="MFDRSRRDSRGPDPDILRSPAPAPGKSTLTGSLPQPAATGVVQM
                     SAASHPAPVTEQAQVAGPNKNKTINWDDPPGLYGIAGGDKTALRTLYRQHLREHMALE
                     PRMIELGYQALAPSEVGARIEAIADRSNPLWVGGKQITGPDAAVPYREALWRCSQRAV
                     VDTLATTASDTRYKKDKVPVMKDGKPVLDDNGQPKLVDQTYCNVYSADMVNAMGGYLP
                     RVWYSNDNLDSATRLPADQLKGKMELSANQIG"
     gene            894..>1988
                     /gene="gcvT"
                     /locus_tag="VF516_44580"
     CDS             894..>1988
                     /gene="gcvT"
                     /locus_tag="VF516_44580"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEX8114911.1"
                     /translation="MERKRTPLWQVHKQLGARLIDFGGWDMPVTYPAGTIKEHRAVRE
                     AVGLFDVSHMGEAVLRGPRVREAVERLTTNAVASAPPGKAVYTLLCRPDGGVVDDCIF
                     YKRSDEEFFVIVNASNTAKDLDWFRDHTHSLCDIDDVSDYTALIAVQGPKAVGLVDQL
                     AGGVLAGLPSFGFTNAVVAGVECLAARTGYTGEDGFELACTADEAVALWTALMDNLPP
                     GGLPIGLGARDTLRLESRLPLYGNDLDDDHTPLEAGLGWAVKLDREFIGAQVLREQKQ
                     RGLTRQLAGFKIAEDARAIARHGYAVVDRSLGAGEAAVIGTVTSGGPGICVGGSIGLA
                     YVPVARAKPGTELTIDCRGKDTPATVVSGKF"
BASE COUNT          250 a          697 c          730 g          311 t
ORIGIN      
        1 cgccgatctg gttcgcgctg agctccatct tgcccttcag ctggtctgcg ggcagcctgg
       61 tcgcgctgtc gaggttgtcg ttgctgtacc agacgcgcgg gaggtagccg cccatcgcgt
      121 tgaccatgtc cgcgctgtag acgttgcagt aggtctggtc gacgagcttg ggctggccgt
      181 tgtcgtcgag caccggcttg ccgtccttca tgaccgggac cttgtccttc ttgtagcggg
      241 tgtccgacgc ggtggtcgcc agcgtgtcga cgaccgcgcg ctgcgagcac cgccacagcg
      301 cctcgcggta cggcaccgcc gcgtcggggc cggtgatctg cttgccgccg acccacagcg
      361 ggttcgaccg gtcggcgatc gcctcgatgc gcgcgccgac ctcgctcggc gccagcgcct
      421 ggtagccgag ctcgatcatc cgcggctcca gcgccatgtg ctcgcgcagg tgctgccggt
      481 acagcgtgcg cagcgccgtc ttgtcgccgc ccgcgatgcc gtacagcccg ggtgggtcgt
      541 cccagttgat cgtcttgttc ttgttcggcc cggcgacctg ggcctgctcg gtcaccgggg
      601 ccgggtggga cgccgccgac atctgcacca cgccggtcgc ggcgggctgt gggaggctgc
      661 cggtcagcgt cgacttgccg ggagccggcg caggggatcg gaggatgtcg gggtcggggc
      721 cgcgggagtc gcgccgggat cggtcaaaca tgcatcgcaa gacgcagccg gcgccgattg
      781 tttcctgtcc ggcgccgcga tccgcgatgg cggcggatcg acgctgcgcg ttgcgcggag
      841 ttgggggccc gggcgcgccc ccggtttgcc cggcgtgcta gtgtccgccc gctatggaac
      901 gcaagcgaac gccgctgtgg caggtgcaca agcaactcgg cgcgcgcctg atcgacttcg
      961 ggggctggga catgcccgtc acctacccgg ccgggacgat caaggagcac cgggcggtcc
     1021 gcgaggcggt cggcctgttc gacgtctcgc acatgggcga ggcggtgctg cgcggtccgc
     1081 gggtgcgcga ggcggtcgag cggctcacga ccaacgcggt cgcgtcggcg ccgcccggca
     1141 aggcggtcta cacgctgctg tgccggcccg acggcggcgt ggtcgacgac tgcatcttct
     1201 acaagcgctc cgacgaggag ttcttcgtca tcgtcaacgc gtcgaacacc gccaaggacc
     1261 tcgactggtt ccgcgaccac acccacagcc tgtgcgacat cgacgacgtc agcgactaca
     1321 cggcgctgat cgcggtgcag ggccccaagg cggtcgggct ggtcgatcag ctcgccggcg
     1381 gcgtgctcgc cgggctgccc agcttcggct tcaccaacgc ggtggttgcc ggcgtcgagt
     1441 gcctggccgc gcgcaccggc tacaccggcg aggacggctt cgagctcgcg tgcaccgccg
     1501 acgaggcggt ggcgctgtgg accgcgctga tggacaacct gccgcccggc ggcctgccga
     1561 tcggcctggg cgcccgcgat accctgcgcc tggagtcgcg gctgccgctc tacggcaacg
     1621 acctcgacga cgaccacacc ccgctcgagg ccggcctggg ctgggcggtc aagctcgacc
     1681 gcgagttcat cggcgcccag gtgctgcgcg agcagaagca gcgcggccta actcgccagc
     1741 tcgcgggctt caagatcgcc gaggacgcgc gcgcgatcgc gcggcacggc tacgcggtgg
     1801 tcgaccgctc gctcggcgcc ggcgaggccg cggtgatcgg cacggtcacc agcggcggcc
     1861 cggggatctg cgtcggcggg tcgatcggcc tggcctacgt cccggtggcg cgggccaagc
     1921 ccggcaccga gctcacgatc gattgccgtg gcaaggacac ccccgcgacg gtggtgtccg
     1981 gcaagttc
//
LOCUS       DASVOR010002427         5790 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_143390,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002427 DASVOR010000000
VERSION     DASVOR010002427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143390"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2499)
                     /gene="dnaE"
                     /locus_tag="VF516_44585"
     CDS             complement(<1..2499)
                     /gene="dnaE"
                     /locus_tag="VF516_44585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEX8114912.1"
                     /translation="MAGFTHLHVHTQYSLLDGAIRVKDLFPKVKDLGMDTVAVTDHGN
                     MFGAIDLYTEAKQSGVKLIFGCETYVAATDRHDRTNRRNFHLILLAKDDAGYKNLSFL
                     NSKGYLEGFYYNPRIDKQLLRERSEGLIGMSACLGGEIAQTLEKNGVAAAEEVAKEYA
                     SMFAPGDFYLELMPTPTPEQETLNGELVRMSKKLGIPLVATNDCHYVNRGDATAHDVL
                     MAIQTGKSLKDEKRLKHVVDSYYLKAPSEMDTAFKDVPEAVENTAAIAARCNVKLKLD
                     ETFLPAYKVPEGETLDSYIATLIEQGLERRFKELASRGIAFDPDRYRERCRSELAVIQ
                     KMGFSGYFLIVWDFINWAKDHGIPVGPGRGSGAGSVVAWALRITDIDPLEFKLLFERF
                     LNPERVSMPDFDVDFCMNRRGEVIKYVQDKYGKDQVGQIATFHQLKARGVIRDIARVM
                     EIPFAEADKLAKLVPEPVQGKSPPVREAIEQTPELKQLYNESPLHRELLDLAAALEGL
                     NRNAGMHAAGVVIAERPLWDYVPCFRGQNDEIVTQFAMKEVEKAGLVKFDFLGLKTLT
                     VIQTAVKLINQQRPPDDDFDIDTIRKDDPDVFKMIARGDTTGVFQLESSGFREILKKL
                     KPDCLEDIVAAVALYRPGPLEGGMVDDFIDRKHGKKKVEYPHPWLEQVLADTYGVIVY
                     QEQVMQIAQILGGYSLGGADLLRRAMGKKKPEEMAKQKETFLAGARAKSVDPRVADQV
                     FELMAFFAGYGFNRSHSAAYGWITYQTAYLKHHFPHEFMAGLMSCDADNIDNVVKFIA
                     EARAMGLVVERPDINESLADFTVTTSAGTEGRIEG"
     gene            3016..4908
                     /locus_tag="VF516_44590"
     CDS             3016..4908
                     /locus_tag="VF516_44590"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEX8114913.1"
                     /translation="MHILRFREKKSLANARKNRLEIIKAQLSRREMIRAGLIGAGGYL
                     VLKNGLSQWASGHEAWASGGSGGSDGSGSGSGTTVVSPPTRAFIEALPIMPIHQPVTT
                     LTGPTPTIAPNTAGGEGRTRPHQAFSAYPSHFTFPPALKFEVHQKAATVIMSPDLPPQ
                     PIWGFDGRYPAPTYYGRYGQGQDVLVRNFNDLPPNSQNGGFGMNTVSTHLHNAHTPSE
                     SDGFPCDFFAPGQFYDHHYPHALAGFGSTHVSTNGDINEALSTLWYHDHRVDFTAQNV
                     YKGLTGFYCLYNQFDTGNETTGFRMPGVQASTNFYTQLLYDVPLMLADRVFDPTTGLL
                     LFDLFNTDGILGDKFLVNGKIQPFFQVEPRRYRFRLLNSGPSRFYQVFLTDQGTNTQI
                     PFWQIGTDGNLLPHPISVTNTIMGVAQRMDIIIDFKPWAGKTLYFENRMEQTDGRGPT
                     GNTLAAGTGNFLMQFRVGTTVTHADASVDPATRPTYYSLPSTAATPRASRTFQFSRSN
                     GMWQINGQIMSNDCSQVRLQVQQNSVEHWTLQNNSGGWMHPVHIHFEEHQIMSQNLPL
                     LNTDVSRKDVMRLQHNETATLFFRFRDFTGRYPLHCHNVVHEDHAMMLRWDIVPAPGG
                     DTNSNP"
     gene            4937..5533
                     /locus_tag="VF516_44595"
     CDS             4937..5533
                     /locus_tag="VF516_44595"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEX8114914.1"
                     /translation="MAKETPDAPSPRQWGSVSPREVIRRRYFPDVTLVTHEGKKVRLY
                     EDLIKDKCVMMNFMYARCTGICSPVTANLKRAQALLKDHIGRDMFMYSFTLKPEEDSP
                     AALNEYVKERKLGPGWTFLTGHPDDLELVRRRLGFSDLDPKTDADKSSHTGIVRYGNE
                     PLMLWAAFPGVQLPQAIARSMMWVAAPGKADPKFLRRE"
BASE COUNT          973 a         1976 c         1830 g         1011 t
ORIGIN      
        1 gccctcgatt ctgccctcgg tgcccgccga cgtggtgacc gtgaagtcgg ccagcgactc
       61 gttgatgtcc gggcgctcga ccaccagacc catcgcgcgg gcctcggcga tgaacttgac
      121 gacgttgtcg atgttgtccg cgtcgcacga catcaggccc gccatgaact cgtgcgggaa
      181 gtggtgcttg aggtacgcgg tctggtacgt gatccagccg tacgccgccg agtgcgagcg
      241 gttgaacccg tagccggcga agaacgccat cagctcgaac acctgatcgg cgaccctggg
      301 gtcgacgctc ttggccctgg cgccggcgag gaaggtctcc ttctgcttgg ccatctcctc
      361 gggcttcttc ttgcccatcg cgcggcgcag caggtcggcg ccgccgagcg agtagccgcc
      421 caggatctgg gcgatctgca tgacctgctc ctggtacacg atcacgccgt aggtgtcggc
      481 gagcacctgc tcgagccagg ggtgcggata ctcgaccttc ttcttgccgt gcttgcgatc
      541 gatgaagtcg tcgaccatgc cgccctcgag cggccccggc cggtacagcg cgaccgcggc
      601 gacgatgtcc tccaggcagt ccggcttgag cttcttcagg atctcgcgga agccgctcga
      661 ctcgagctgg aacacgccgg tggtgtcacc gcgcgcgatc atcttgaaca cgtccgggtc
      721 gtccttgcgg atcgtgtcga tgtcgaagtc gtcatccggc ggccgctgct ggttgatcag
      781 cttgaccgcg gtctggatca ccgtgagcgt cttgaggccg aggaagtcga acttgaccag
      841 gccggccttc tcgacctcct tcatcgcgaa ctgggtgacg atctcgtcgt tctggccgcg
      901 gaagcacggc acgtagtccc acagcggccg ctcggcgatc accaccccgg cggcgtgcat
      961 gccggcgttg cggttgaggc cctcgagcgc ggcggcgagg tcgagcagct cgcggtgcag
     1021 cgggctctcg ttgtacagct gcttgagctc gggggtctgc tcgatcgcct cgcggaccgg
     1081 cggcgacttg ccctggaccg gctcgggcac gagcttggcg agcttgtcgg cctcggcgaa
     1141 cgggatctcc atgacccgcg cgatgtcgcg gatcacgccg cgcgccttga gctggtggaa
     1201 cgtcgcgatc tggccgacct ggtccttgcc gtacttgtcc tggacgtact tgatgacctc
     1261 gccgcgccgg ttcatgcaga agtcgacgtc gaagtccggc atcgacacgc gctcggggtt
     1321 gaggaagcgc tcgaacagca gcttgaactc gagcgggtcg atgtcggtga tccgcagcgc
     1381 ccacgccacc accgagcccg cgcccgagcc gcggcccggg ccgaccggga tgccgtggtc
     1441 cttggcccag ttgatgaagt cccagacgat caggaagtag cccgagaacc ccatcttctg
     1501 gatcacggcg agctcggacc tgcagcgctc gcgatagcga tccggatcga acgcgatgcc
     1561 gcggctcgcg agctccttga accggcgctc caggccctgc tcgatcagcg tggcgatgta
     1621 cgagtcgagg gtctcgccct ccgggacctt gtaggccggc aggaaggtct cgtcgagctt
     1681 gagcttgacg ttgcagcgcg cggcgatcgc agcggtgttc tcgaccgcct cggggacgtc
     1741 cttgaacgcg gtgtccatct cggacggcgc cttcaggtaa tagctgtcga ccacgtgctt
     1801 gagccgcttc tcgtccttga ggctcttgcc ggtctggatc gccatcagca cgtcgtgcgc
     1861 ggtggcatcg ccgcggttga cgtagtggca gtcgttggtc gcgaccagcg ggatgccgag
     1921 cttcttgctc atccgcacca gctcgccgtt gagcgtctcc tgctccggcg tcggcgtcgg
     1981 catcagctcg aggtagaagt cgccgggcgc gaacatgctc gcgtactcct tggcgacctc
     2041 ctcggccgcg gcgacgccgt tcttctcgag cgtctgcgcg atctcgccgc cgaggcacgc
     2101 cgacatgccg atcaggccct cgctgcgctc gcgcagcagc tgcttgtcga tccgcgggtt
     2161 gtagtagaag ccctccaggt agcccttgga gttgaggaac gacaggttct tgtagccggc
     2221 gtcgtccttg gcgagcagga tcaggtggaa gttcctgcgg ttggtgcggt cgtggcgatc
     2281 ggtcgcggcg acataggttt cgcaaccgaa aatcaatttc acacccgact gcttggcctc
     2341 ggtatagaga tcgatggccc cgaacatgtt gccgtgatcg gtgaccgcca ccgtgtccat
     2401 tccgaggtcc ttgaccttgg ggaacaggtc cttcacccgg attgcgccgt cgagcaggct
     2461 gtactgggtg tggacgtgaa gatgggtgaa tccggccatg gcgatcgccc tgatctagcg
     2521 catccccccg acactcccgc gcgatccagg ccggcgttcc cgaggacctg tgtaattccg
     2581 tacagatgaa ccagagccag cgactgatca gggttcaacg tcttctttaa gatcaggtta
     2641 caactggagt tttcgggcat gtgcgtgatc gcgcacacgt tgtcgatctc catgtggaaa
     2701 atttgcacag tggtgtgcaa cacgcggcgc ccggtcatca gctccggcgc ttctcacatc
     2761 gcgccacagt cattatgccc cgatccgaat cgcgtcacgt gtggagcgat actgaaagcc
     2821 actttcagga ccggagtcag gactcgattg ttgctgggat tctggcgaat ccgttccgga
     2881 tcatgccgtt ttcatcaaca accaatattt ccttgtttta agcacttttc gtggacggcg
     2941 catttctgac gtgaaaaaag cagttctgca cagaaatctg aggccgcaac tcggccgcta
     3001 tcccggaagg tccccatgca catcctccgc tttcgtgaaa agaaatccct cgcgaacgct
     3061 cgcaagaacc gtctggagat catcaaggcc cagctcagcc gccgcgagat gatccgggcc
     3121 ggtctgatcg gggccggcgg ctacctggtg ctcaagaacg ggctcagcca gtgggcctcg
     3181 ggccatgaag cgtgggcgag cggcggcagc ggcgggagtg acggcagcgg gagcggcagc
     3241 ggcacgacgg tcgtcagccc gccgacccgg gcgttcatcg aggcgctgcc gatcatgccg
     3301 atccaccagc cggtcaccac gctgaccggg ccgaccccga cgatcgcgcc caacaccgcc
     3361 ggcggcgagg gccgcacgcg gccgcaccag gcgttctcgg cctatccctc gcacttcacc
     3421 ttcccgccgg ccctcaagtt cgaggtccac cagaaggccg cgaccgtgat catgtccccg
     3481 gacttgccgc cgcagccgat ctggggcttc gacggccgat acccggcgcc cacctactac
     3541 gggcgctacg gccagggcca ggatgtgctg gtccgcaact tcaacgacct gccgccgaac
     3601 tcgcagaacg gcgggttcgg catgaacacg gtctcgaccc acctgcacaa cgcgcacacc
     3661 ccgtcggaga gcgatggctt cccgtgcgac ttcttcgcgc cgggccagtt ctacgaccac
     3721 cactaccccc acgcgctcgc cgggttcggc tcgacccacg tctcgaccaa cggcgacatc
     3781 aacgaggcgc tgagcacgct gtggtaccac gaccaccgcg tcgacttcac ggcgcagaac
     3841 gtctacaagg gcctgaccgg gttctactgc ctgtacaacc agttcgacac cggcaacgag
     3901 accaccgggt tccggatgcc gggagtccag gcgtcgacga acttctacac gcagctgctc
     3961 tacgacgtgc cgctgatgct cgccgaccgc gtgttcgacc ccaccaccgg cctcttgttg
     4021 ttcgatctgt tcaacaccga cggcatcctc ggcgacaagt tcctggtcaa cggcaagatc
     4081 cagccgttct tccaggtcga gccgcggcgc taccggttcc gcctgctcaa cagcggtccg
     4141 tcgcggttct accaggtgtt cctgaccgac cagggcacca acacccagat cccgttctgg
     4201 cagatcggca ccgacggcaa cctgctgccc cacccgatca gcgtcaccaa caccatcatg
     4261 ggcgtggcgc agcggatgga catcatcatc gacttcaagc cgtgggccgg caagacgctg
     4321 tacttcgaga accgcatgga gcagaccgat ggccgcggcc cgaccggcaa cacgctggcc
     4381 gccggcaccg gcaacttcct gatgcagttc cgggtcggga ccacggtgac ccacgccgac
     4441 gccagcgttg atccggcgac ccgcccgacg tactactcgc tgccgagcac ggccgcgacg
     4501 ccgcgggcgt cgcgcacctt ccagttctcg cgcagcaacg gcatgtggca gatcaacggc
     4561 cagatcatgt cgaacgactg ctcgcaggtc cggctgcagg tccagcagaa cagcgtcgag
     4621 cactggaccc tgcagaacaa ctccggcggc tggatgcacc cggtgcacat ccacttcgag
     4681 gagcaccaga tcatgagcca gaacctgccg ctgctcaaca ccgacgtgtc gcgcaaggac
     4741 gtgatgcggc tccagcacaa cgagaccgcc acgctgttct tccggttccg cgacttcacc
     4801 gggcgctacc cgctgcactg ccacaacgtc gtccacgagg accacgcgat gatgctgcgc
     4861 tgggacatcg tgccggcgcc cggcggtgac accaactcga acccctgagc cacggcggcc
     4921 gacgaaagga cacaccatgg ccaaggaaac cccagatgcg ccgagcccca ggcagtgggg
     4981 gtcggtgtcg ccgcgcgagg tcatccggcg ccggtacttc ccggacgtca cgctggtgac
     5041 ccacgagggc aagaaggtcc ggctctacga ggatctgatc aaggacaagt gcgtcatgat
     5101 gaacttcatg tacgcgcggt gcacgggcat ctgcagcccg gtgacggcca acctgaagcg
     5161 cgcgcaggcg ctgctcaagg accacatcgg gcgcgacatg ttcatgtact cgttcacgct
     5221 caagcccgag gaggactcgc cggcggcgct caacgagtac gtgaaggagc gcaagctcgg
     5281 gccgggctgg acgttcctca ccggccaccc cgacgatctg gagctggtgc gccgccggct
     5341 cgggttcagc gacctggatc ccaagaccga tgccgacaag agcagccaca ccggcatcgt
     5401 gcgctacggc aacgagccgc tgatgctgtg ggccgcgttc cccggcgtgc agctgcccca
     5461 ggcgatcgcc aggtcgatga tgtgggtcgc cgcgcccggc aaggccgatc cgaagttcct
     5521 gcgcagggag tgatcgcgcg gcgcagacgc ggcgtccgat cgtgctaaag acggtctgac
     5581 gttgctccac ggggggctct gccccccgcc ccgccggccg agccggcagg gtccccaccc
     5641 ccgccgactc gcttcgctcg tcgatcatgc gcccgatctt cccgcggctc gcgggccgcc
     5701 tctgctcgcc tgcctcgccg cgcggcgtac gcgccgtggt tcgctgcggc ggcggcactg
     5761 ttggcctcgg cctcggggcg ctcggtatgg
//
LOCUS       DASVOR010002428         1846 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_494508,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002428 DASVOR010000000
VERSION     DASVOR010002428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1846
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494508"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1596)
                     /locus_tag="VF516_44600"
     CDS             complement(<1..1596)
                     /locus_tag="VF516_44600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114915.1"
                     /translation="MTIAPDRQRVAGVSAEADELTICADPYDPRCNSDNFDFEAWLAG
                     HDPDLFATAEGRRAATRADPMLFALTYLRHHLTDKDTGEISFADCHLDWYRYARQWMI
                     KDTGLRGWRHSFASPRNSAKSTTWYTLVPIWAGAHGHVKFLASFADSGTQAEMHLQTF
                     RTEAQHNRLLRNDFPDLCTAIRKPTGRTVADNEGQYQSKSGFVWVARGIDTSTLGMKV
                     GDRRPDVIILDDIEKDESNYSPLEVEKRRKTLIDAILPLNERAKVVLVGTVTRPGSIN
                     HQLVKVALSEVDPASEEGEALSWIGDERIKPHYYAPILERSDGSRRSIWPAKWSLEYL
                     ESIEHTRNYAKNFANNPRAESGPFWSGADIRYSRPVNVARQYLFVDPPTTTKTTSDPA
                     GLAVLGYAPGTGKLPSLEDRKRLKHLLGDDWRDTLTREEAEAQGLARLARVVVEHAEE
                     VRATGTPLKLRVLTLLERFPGIVAVVIEVNQGGDLWKEIMDELPVKLITFHSSEPKIV
                     RIGWGLEMYQKRRVTHAKRLPAAE"
     gene            complement(1593..>1846)
                     /locus_tag="VF516_44605"
     CDS             complement(1593..>1846)
                     /locus_tag="VF516_44605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114916.1"
                     /translation="ALPGRTGQTMIFRTGRHLGRTIYVQLDDEPDRTRDVEVGMVDTP
                     ALGSLVCTALNAYAQSGDLPHRQVETIIRDVLAWRRIRP"
BASE COUNT          254 a          590 c          671 g          331 t
ORIGIN      
        1 ctcggccgcc ggcagccgct tggcgtgcgt gacgcggcgc ttctggtaca tctccaggcc
       61 ccagccgatc cggacgatct tgggctcgct ggaatggaag gtgatcagct tcaccggcaa
      121 ctcgtccatg atctctttcc agaggtcgcc gccctggttg acctcgataa ccacggcgac
      181 gataccgggg aagcgctcca gcagcgtcaa cacgcgcagc ttgagcggcg tgccggtggc
      241 ccgcacctcc tcggcgtgct cgacgaccac ccgggccagc cgggccagtc cctgcgcctc
      301 ggcctcctcg cgggtcaggg tgtcgcgcca gtcgtcgccg agcaggtgct tgagccgctt
      361 gcggtcctcc agcgagggca gcttgccggt gccgggggcg tagccgagca cggcgaggcc
      421 ggccgggtcg ctggtcgtct tcgtggtggt cggcgggtcg acgaacaggt actgccgcgc
      481 cacgttgacc ggccggctgt accggatgtc ggcaccggac cagaacgggc cggactcggc
      541 gcgcgggttg ttggcgaagt tcttggcgta gttgcgggtg tgctcgatcg actccaggta
      601 ctccagggac cacttggccg gccagatcga gcgccggctg ccgtcggagc gctccaggat
      661 cggcgcgtag tagtgcggct tgatccgctc gtcgccgatc cacgagaggg cctcgccctc
      721 ctcgctggcc gggtcgacct cggacagggc caccttgacc agttggtggt tgatcgagcc
      781 gggccgggtg acggtaccga ccagcacgac cttggcccgc tcgttcagcg gcaggatcgc
      841 gtcgatcagg gtcttgcgcc gcttctcgac ctccaggggc gaatagttcg actcgtcctt
      901 ctcgatgtcg tcgaggatga tcacgtcggg gcgccggtcg cccaccttca tgcccagtgt
      961 cgaggtgtcg atgccccggg ccacccacac aaagccgctc ttggactggt attgcccctc
     1021 gttgtcggcc acggtgcgcc cggtcggctt gcggatcgcc gtacacaggt ccgggaagtc
     1081 gttgcgcaac agccgattgt gttgggcctc ggtgcggaac gtctggaggt gcatctccgc
     1141 ctgggtcccg gagtcggcga agctggccag gaatttgacg tgtccgtggg cgccggccca
     1201 gatcggcacg agcgtgtacc aggtggtcga cttggccgag ttgcgggggc tggcgaagct
     1261 gtgccgccag ccgcgcagcc cggtgtcttt gatcatccat tgacgggcgt agcggtacca
     1321 gtcgaggtgg cagtcggcga agctgatctc accggtgtcc ttgtcggtga ggtggtggcg
     1381 caggtaggtg agcgcgaaca gcatcgggtc ggcacgggtg gccgccctgc gcccctcggc
     1441 ggtggcgaac aggtcggggt cgtgaccggc cagccaggct tcgaagtcga agttgtcgct
     1501 gttgcagcgg ggatcgtacg ggtcggcgca gatggtgagt tcgtcggcct cggcgctcac
     1561 gccggccacc cgctggcggt ccggcgcgat ggtcatggtc ggatccgacg ccaggcgagc
     1621 acatcccgga tgatcgtctc gacctgacgg tggggtaggt cgccggactg ggcgtaggcg
     1681 ttcagggcgg tgcacacgag cgaaccgagc gccggagtgt cgaccatgcc cacctcgacg
     1741 tcccgggtcc ggtcgggctc gtcgtcgagc tgcacataga tggtgcgtcc gagatggcgg
     1801 ccagtgcgga agatcatggt ttgtccagtc cgcccaggta gcgccc
//
LOCUS       DASVOR010002429         1664 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_405635,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002429 DASVOR010000000
VERSION     DASVOR010002429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405635"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..228
                     /locus_tag="VF516_44610"
     CDS             <1..228
                     /locus_tag="VF516_44610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114917.1"
                     /translation="RRTPAGQAAAGQASTGITVGLTLGGTPPATQPPGAADDADDEPG
                     DDAAAADDSAEDTAADDAADDQAATDDPSDD"
     gene            291..>1664
                     /locus_tag="VF516_44615"
     CDS             291..>1664
                     /locus_tag="VF516_44615"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8114918.1"
                     /translation="MDRGSGRRLPALELAVVAGDAVTTAALPAAGSVTLGRDAECDVR
                     IDNRSVSRRHAVLHLGPPLQIQDLGSANGTFVRDARSPVDTTATHPLRRLSGEILEIA
                     IGERVNLGAIPIVVRRARAAPASATPPDAVIVRDPAMRALYDQVTRAARAAISVLILG
                     ETGVGKEVFARAIHDRSRRAGGPFLELNCAALPAGLLEGELFGHEKGAFTGASQATPG
                     LLESADGGTVFLDEIGELPAAVQVKLLRVLEDRKVLRIGGRTPRTLDVRFVAATNRDL
                     QAEVERGNFRQDLYFRLNGVAFAIPPLRERVTEIQPLAERFLAAAGEMLDRGEPLRLS
                     AEALGYLERYRWPGNVRELRNVIERAAVLSLGSAIEPAGLPPPITRAAIDPGSLSGRE
                     DSGARGDRPDRSDPAPDGDRQRIIAALEQCAGNQTRAAKLLGISRRTLVNRLASYDIP
                     RPRPRAGK"
BASE COUNT          216 a          658 c          596 g          194 t
ORIGIN      
        1 cgccggacac ccgccggcca ggcagccgcg ggccaggcgt ccaccggcat caccgtggga
       61 ctgacgctgg ggggcacacc gccggcgacc cagccccccg gggcggccga cgacgccgac
      121 gacgagcccg gcgacgacgc cgcggcggcc gacgactccg ccgaggacac ggcggccgac
      181 gatgcagccg acgatcaggc cgcgaccgat gacccgagcg acgactgacc gcgaccgatg
      241 accggctcac cgcgctctcg agccgcgggc gaggcagtac aatcgcccgg gtggatcgag
      301 gctccggtcg ccggcttcca gcgctcgagc tcgccgtggt ggcgggcgac gcggtcacca
      361 ccgccgcgct cccggccgcc ggctcggtga cgctcggccg cgacgcggag tgcgatgtcc
      421 gcatcgacaa tcgctcggtg tcccgccgcc acgccgtgct ccacctcggc ccgccgctgc
      481 agatccagga cctcggcagc gccaacggca ccttcgtgcg cgatgcgcgg tcgccggtcg
      541 acaccaccgc gacccacccg ctgcgccgcc tgtccggcga gatcctcgag atcgcgatcg
      601 gcgagcgcgt gaacctcggc gcgatcccga tcgtcgtccg ccgcgcccgg gccgccccgg
      661 cctccgccac gccgcccgac gcggtcatcg tgcgcgatcc ggccatgcgc gcgctctacg
      721 accaggtcac ccgggccgcg cgggccgcga tcagcgtgct catcctcggc gagaccggcg
      781 tcggcaagga ggtcttcgcg cgcgcgatcc acgaccggtc gcggcgcgcc ggcgggccgt
      841 tcctcgagct caactgcgcg gcgctgccgg cggggctgct cgagggcgag ctgttcggtc
      901 acgagaaggg cgcgttcacc ggggcgagcc aggcgacccc cggcctgctc gagtcggccg
      961 acggcggcac ggtgttcctc gacgagatcg gcgagctgcc ggcggccgtg caggtcaagc
     1021 tgctgcgcgt gctcgaggac cgcaaggtgc tgcggatcgg cggccggacg ccgcggacgc
     1081 tcgacgtccg gttcgtggcc gcgaccaatc gcgatctcca ggccgaggtc gagcgcggca
     1141 actttcgcca ggatctgtac ttccggctca acggcgtggc gttcgcgatc ccgccgctgc
     1201 gcgagcgcgt caccgagatc cagccgctcg ccgagcgctt cctggccgcc gccggcgaga
     1261 tgctcgaccg cggcgagccg ctgcggctgt cggccgaggc gctgggctac ctcgagcgct
     1321 accgctggcc gggcaacgtg cgcgaactgc gcaacgtgat cgagcgcgcc gcggtgctgt
     1381 cgcttggcag cgccatcgag cctgcgggtc tcccgccgcc gatcacgcgc gcggcgatcg
     1441 accccggttc gctgtccggt cgcgaggata gcggcgctcg cggtgaccgc ccggaccgca
     1501 gcgaccctgc gcccgacggc gaccgccagc ggatcatcgc ggcgctcgag cagtgcgccg
     1561 gcaaccagac ccgcgccgcg aagctgctcg gcatctcgcg gcgcacgctg gtcaatcgcc
     1621 tcgccagcta cgacatcccg cgcccgcgac cccgcgctgg caag
//
LOCUS       DASVOR010002430         2793 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_347500,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002430 DASVOR010000000
VERSION     DASVOR010002430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347500"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..284
                     /locus_tag="VF516_44620"
     CDS             42..284
                     /locus_tag="VF516_44620"
                     /inference="COORDINATES: protein motif:HMM:NF015807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Arc family DNA-binding protein"
                     /protein_id="HEX8114919.1"
                     /translation="MADQITVRNVPKELARRLAKLAEASGRSINATVLDLLERATGID
                     GRRARLERYTTWSKADAEDLEHALREQRTIDPKLWE"
     gene            285..680
                     /locus_tag="VF516_44625"
     CDS             285..680
                     /locus_tag="VF516_44625"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEX8114920.1"
                     /translation="MRIVLDTSAYSHFRSNHAGVVDLLARADRIYLPTIVLGELEAAF
                     RRGRRTSDNRAKLDEFLREDFVGVLPVTTDVARRYGELFAELRAAGTPIPINDVWIAA
                     TTIDAGAELVTFDTDFEQVARLERTILVP"
     gene            762..2585
                     /locus_tag="VF516_44630"
     CDS             762..2585
                     /locus_tag="VF516_44630"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEX8114921.1"
                     /translation="MVAPYVDSPEAEGDRPRDRRRLAVLWRLLPLIWPHRARFVLALV
                     ALCGASAINLVYPWAARQVVDIGLTKQSTGELDQILVLMVGVFLVNAALVWLRHYSIS
                     WLGERVVADLRGLVFDRVLTLPLAWFHERRSGELVGRLASDVTVVEGVVGSQLSMALR
                     NGLQLIGALVMLFVIDVKLTLLMLAIVPPITLATIYFGRRIRKMARAVQDALAKVSGA
                     AQESIGAIATVQAFVRESYEAGRYRGGVEQAFDQTLAMVRWRSWFMSTAMTAGYVGVT
                     AVIWIGGRALINAHDPDGGLTPGQLLSFFLYPFIGAGALGDVSDLWSALQRAAGATDR
                     LFAVIDTVPAIRDPEPGRALPPGKGAIKLERVSFAYPSRRGQPVLTDVDLEVAPGEVV
                     ALVGPSGAGKSTILSLLYRFYDVDAGRVLLEGVDVRELRLGELRRALAMVAQEPVLFS
                     GTIRDNIGYGRAGATPAEIERAARDAHAHDFITGFPDGYATMIGERGTKLSGGQKQRI
                     ALARALLADPRVLILDEATSNLDAESEAAVQAALARLMHGRTTIIVAHRLSTVRDADR
                     IVVIEGARVVEQGRHDELMARRGTYHRLVEHQLIADHRAAS"
     gene            2582..>2793
                     /locus_tag="VF516_44635"
     CDS             2582..>2793
                     /locus_tag="VF516_44635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114922.1"
                     /translation="MTGFETAFETIGLDLPGGAFHALAAGTGRPVLYHHGFPDHPPTA
                     RAFLGELARERDPLLLAARQLGAALA"
BASE COUNT          381 a          968 c         1015 g          429 t
ORIGIN      
        1 cctttgacat catgcctgca tgatagtaga ttcatgatgt catggccgac cagatcaccg
       61 tgcgcaatgt ccccaaggag cttgctcgtc ggctggcaaa gctcgccgaa gcgagcggtc
      121 ggagcatcaa tgcgaccgtg ctggatctcc tggagcgcgc aaccgggatc gacggccggc
      181 gcgcgcggct ggagcgctac accacatggt cgaaggccga tgccgaggac ctcgagcacg
      241 cgttgcgcga gcagcgcacc atcgacccca agctctggga gtagatgcgc atcgtcctcg
      301 acacatcggc gtactcgcac tttcgtagca accacgccgg cgtggttgac ctgctggcca
      361 gggccgaccg gatctacttg ccgacgatcg tcctcggcga gctcgaggct gcattccggc
      421 gcggtcggcg cacgagcgat aaccgcgcga agctggacga atttcttcgc gaggacttcg
      481 tgggtgttct cccggtgaca accgacgttg ccagacgcta cggggaactg ttcgccgagt
      541 tgcgcgccgc gggcacgccg attccgatca acgacgtctg gatcgccgcg acgacgatcg
      601 acgcgggtgc cgagctcgtc accttcgata ccgacttcga gcaggtcgcg cgactggagc
      661 gcacgatcct cgtgccgtga caccgtcgcc gagccagtga gtgcgcatcg gcgggttgtt
      721 ctccaggtga cagcggccag acggcgcgct atgcagccgc ggtggtggca ccgtacgtgg
      781 actctccgga ggccgagggg gatcggccgc gcgatcgccg ccggctggcg gtgctgtggc
      841 ggctgctgcc gctgatctgg ccgcaccgcg cgcggttcgt gctcgcgctg gtcgcgctgt
      901 gcggggcgtc ggcgatcaac ctcgtgtatc cgtgggcggc gcgccaggtc gtcgacatcg
      961 ggctgaccaa gcagagcacg ggcgagctcg accagatcct ggtgctgatg gtcggcgtgt
     1021 tcctggtcaa cgccgcgctg gtctggctgc gccactacag catcagctgg ctcggcgagc
     1081 gcgtggtcgc cgacctgcgc ggcctggtgt tcgaccgcgt gctgaccctg ccgctggcgt
     1141 ggttccacga gcggcgcagc ggcgagctgg tcggccggct cgcgtcggac gtcacggtgg
     1201 tcgagggcgt ggtcggcagc cagctgtcga tggcgctgcg caacgggctg cagctgatcg
     1261 gcgcgctggt gatgctgttc gtgatcgacg tgaagctgac cctgctgatg ctcgcgatcg
     1321 tcccgccgat cacgctggcg acgatctact tcggccggcg gatccgcaag atggcgcgcg
     1381 cggtgcaaga cgcgctcgcc aaggtctcgg gcgcggccca ggagtcgatc ggcgcgatcg
     1441 ccacggtgca ggcgttcgtc cgcgagtcgt acgaggcggg gcggtaccgc ggcggtgtcg
     1501 agcaggcgtt cgaccagacg ctggcgatgg tgcgctggcg gtcgtggttc atgtcgaccg
     1561 cgatgaccgc gggctacgtc ggcgtgaccg cggtgatctg gatcggcggc cgggcgctga
     1621 tcaacgctca cgaccccgac ggcgggctga ctccggggca gctgctgtcg ttcttcctgt
     1681 accccttcat cggcgccggc gcgctcggcg acgtgtccga cctgtggagc gcgctgcagc
     1741 gcgcggcggg cgccaccgac cggctgttcg cggtgatcga caccgtgccc gcgatccgcg
     1801 atcccgagcc gggccgcgcg ctgccgccgg gcaagggcgc gatcaagctc gagcgggtgt
     1861 cgttcgcgta cccgagccgg cgcggccagc cggtgctgac cgacgtcgac ctcgaggtcg
     1921 cgcccggcga ggtcgtcgcg ctggtcggac cgtcgggcgc cggcaagtcg acgatcctgt
     1981 cgctgctcta ccggttctac gacgtcgacg ccggccgcgt gctgctcgag ggcgtcgacg
     2041 tccgcgagct gaggctcggc gagctgcgcc gcgcgctcgc gatggtcgcc caggagccgg
     2101 tgctgttctc ggggacgatc cgcgacaaca tcggctacgg ccgggccggc gcgaccccgg
     2161 ccgagatcga gcgcgccgcg cgcgacgccc acgcgcacga cttcatcacc gggttccccg
     2221 acggctacgc gacgatgatc ggcgagcgcg gcaccaagct gtcgggcggc cagaagcagc
     2281 ggatcgcgct ggcccgcgcg ctgctcgccg acccgcgcgt gctgatcctc gacgaggcga
     2341 cctccaacct cgacgccgag agcgaggccg cggtgcaggc cgcgctcgcc cgcctgatgc
     2401 acggccgcac cacgatcatc gtggcccacc ggctgagcac ggtgcgcgac gccgaccgga
     2461 tcgtcgtgat cgagggcgcg cgcgtggtcg agcagggccg ccacgacgag ctgatggcgc
     2521 ggcgcgggac ctaccaccgg ctggtcgagc accagctgat cgccgaccac cgggcggcgt
     2581 catgaccggg ttcgagacgg ctttcgagac catcgggctc gacctgccgg gcggggcgtt
     2641 ccacgcgctc gccgccggca ccggccggcc ggtgctctac caccacggct tcccggacca
     2701 cccgccgacc gcgcgcgcgt tcctcggcga gctggcgcgc gagcgcgatc cgctgcttct
     2761 ggccgcccga cagcttggtg ccgcgctcgc cga
//
LOCUS       DASVOR010002431         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_464875,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002431 DASVOR010000000
VERSION     DASVOR010002431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464875"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2400)
                     /locus_tag="VF516_44640"
     CDS             complement(<1..2400)
                     /locus_tag="VF516_44640"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEX8114923.1"
                     /translation="MTPILPWLAATAADAPARPRSEAQLVLPDFTQIHMLGMTGRTIL
                     AIGLVVSAFGLLFGLWKYQKLKNLPVHKAMREISELIYATCKTYLRTQAKFIFQLWVL
                     IAIVIVVYFAALEHVQIAKVIAIAAFSVVGILGSSLVAFFGIRVNTFANSRAAFASLR
                     GKPYPVYEIPIEAGMSIGTVLISTELFLMLAIMVFMPGDYAGPCFIGFAVGESLGAAA
                     LRIAGGIFTKIADIGSDLMKIVVNIKEDDARNPGVIADCTGDNAGDSVGPTADGFETY
                     GVTGVALITFILVAFPNLPGLEAGKLAADGISPADPGQWTQTVLLVWLFAMRIVMIFA
                     SVGSYLINGVLTKGRYGNADKMDFEHPLTNLVWLTTLVSVALTFLVSYYLIGTGDSTL
                     PFGLWWKLSAIISCGTLAGAIIPELVKVFTSVNSGHVREVVTASKEGGPSLNILAGLT
                     AGNFSGYWMGLVITGLMGIAYSISLAPGISELLFSQNQAAGADFSMTAFACQAAGTVF
                     AFGLVAFGFLGMGPVTIAVDSYGPVTDNAQSVYELSVIEQVPNVEQEIEQEFKFKPNF
                     DKAKAYLEENDGAGNTFKATAKPVLIGTAVVGAATMIFALIMLVTNGLKTEAIGNLSM
                     IHPPFLLGLIAGGAIIYWFTGASTQAVSTGAYRAVEFIKANIKLDGTSQKASVEDSNK
                     VVEICTLYAQKGMQNIFMVVFFTTLSFACIDPYFFIGYLISMALFGLYQAIFMANAGG
                     AWDNAKKIVETELRSKGTELHAATVVGDTVGDPFKDTSSVALNPVIKFTTLFGVLAVG
                     LA"
     gene            2567..>3554
                     /locus_tag="VF516_44645"
     CDS             2567..>3554
                     /locus_tag="VF516_44645"
                     /inference="COORDINATES: protein motif:HMM:NF013316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEX8114924.1"
                     /translation="MGDPPAVIRRAPDPGAVPRAPEILAPAGDRDTLAAALAAGADAV
                     YFGLDDGFNARARAANFASADLGEVIAQIHRAGVRACVTLNTLVFEPELPVVEALIRR
                     VAAAGADAIIVQDPAVALLARAICPDLEIHGSTQMTASSPLAAELLRGLGLARVVVPR
                     ELSVDEIRAYAAGTSLPLEVFIHGALCVSWSGQCLSSEAWGGRSANRGQCAQACRLPY
                     QLIVDGRERDLGDVAYLLSPRDLTGLDHVARLAELGVAALKIEGRLKGPQYVATTVAQ
                     YRAAVAAATGESPPTSPVRIPSDEPSLHIAYSRGISRGFLAGPDHQSLIEGRF"
BASE COUNT          572 a         1204 c         1228 g          550 t
ORIGIN      
        1 cgcgaggccg accgcgagca cgccgaacag cgtggtgaac ttgatcaccg ggttgagcgc
       61 gaccgacgac gtgtccttga acggatcgcc gacggtgtcg ccgaccacgg tcgcggcgtg
      121 cagctcggtg cccttggacc gcagctcggt ctcgacgatc ttcttggcgt tgtcccacgc
      181 gccgccggcg ttggccatga agatcgcctg gtacagaccg aacagcgcca tcgagatcag
      241 gtagccgatg aagaagtacg ggtcgatgca ggcgaacgac agggtcgtga agaacacgac
      301 catgaagatg ttctgcatgc ccttctgggc gtagagcgtg cagatctcga cgaccttgtt
      361 ggagtcctcg accgaggcct tctgcgacgt gccgtcgagc ttgatgttgg ccttgatgaa
      421 ctcgaccgcg cggtacgcgc cggtcgacac cgcctgggtc gacgcgccgg tgaaccagta
      481 gatgatcgcg ccgccggcga tcaggccgag caggaacggc gggtggatca tcgacaggtt
      541 gccgatcgcc tcggtcttga ggccgttggt gaccagcatg atcagcgcga agatcatcgt
      601 cgcggcgccg accaccgcgg tgccgatcag cacgggcttg gccgtggcct tgaacgtgtt
      661 gcccgcgccg tcgttctcct cgaggtaggc cttggccttg tcgaagttcg gcttgaactt
      721 gaactcctgc tcgatctcct gctcgacgtt ggggacctgc tcgatcaccg acagctcgta
      781 gaccgactgg gcgttgtcgg tgaccgggcc gtacgagtcg accgcgatcg tcaccgggcc
      841 catgccgagg aagccgaacg cgaccaggcc gaacgcgaac accgtgccgg cggcctggca
      901 cgcgaacgcc gtcatcgaga agtcggcgcc cgcggcctgg ttctgcgaga acagcagctc
      961 ggagatgccg ggtgccagcg agatcgagta cgcgatgccc atgaggccgg tgatgaccag
     1021 gcccatccag tagcccgaga agttgcccgc ggtcaggccg gcgaggatgt tgagcgacgg
     1081 gccgccctcc ttcgacgcgg tcacgacctc gcggacgtgg ccggagttga ccgaggtgaa
     1141 caccttgacc agctcgggga tgatcgcgcc ggcgagcgtg ccgcacgaga tgatcgccga
     1201 cagcttccac cacaggccga acggcagcgt cgagtcgccg gtgccgatca ggtagtacga
     1261 caccaggaag gtcagcgcga ccgacaccag cgtggtcagc cacaccaggt tggtcagcgg
     1321 gtgctcgaag tccatcttgt cggcgttgcc gtagcggccc ttggtcagca cgccgttgat
     1381 caggtagctg ccgaccgacg cgaagatcat gacgatgcgc atcgcgaaca gccagaccag
     1441 cagcacggtc tgcgtccact ggcccggatc ggcggggctg atgccgtcgg cggcgagctt
     1501 gccggcctcg aggccgggca ggttggggaa cgcgaccagg atgaacgtga tcagcgcgac
     1561 gccggtgacg ccgtaggtct cgaagccgtc ggcggtcggg ccgaccgagt cgccggcgtt
     1621 gtcgccggtg cagtcggcga tcacgcccgg gttgcgcgcg tcgtcctcct tgatgttgac
     1681 cacgatcttc atcagatccg agccgatgtc ggcgatcttg gtgaagatgc cgccggcgat
     1741 gcgcagcgcc gcggcgccga gcgactcgcc gaccgcgaag ccgatgaagc acgggccggc
     1801 gtagtcgccg ggcatgaaga ccatgatcgc gagcatcagg aacagctcgg tcgagatcag
     1861 caccgtgccg atcgacatgc cggcctcgat cgggatctcg tagaccgggt acggctttcc
     1921 acgtagcgac gcgaacgccg cgcgcgagtt ggcgaacgtg ttgacccgga tgccgaagaa
     1981 cgcgaccagc gagctgccga ggatgccgac caccgagaac gcggcgatcg cgatcacctt
     2041 ggcgatctgg acgtgctcga gcgccgcgaa gtagaccacg atcacgatcg cgatcagcac
     2101 ccagagctgg aagatgaact tcgcctgggt gcggaggtac gtcttgcagg tcgcgtagat
     2161 cagctcgctg atctcccgca tcgccttgtg gacgggcagg ttcttgagct tctggtactt
     2221 ccagagcccg aacaagagac cgaacgccga caccaccagg ccgatcgcga ggatggttct
     2281 ccccgtcatt cccagcatgt ggatctgtgt gaaatccggc aacacgagct gggcctcgct
     2341 ccgggggcgc gccggcgcgt ccgcggcggt ggctgcaagc catggaagga tcggggtcat
     2401 ggcgggcgtt ataaggagga ggcgattggg tgcgcaaggg ccaagggtgc agcaagcggt
     2461 gattaccgcc tgtgaactgc gtgcgagttt ccgatccgcc acggccggga cgtgttatgg
     2521 aacgctgcca gcaagcgttc gtctgatctg accgccgcct cctgccatgg gcgatccgcc
     2581 cgccgtcata cgccgggcgc ccgatccggg cgccgtcccc cgggcccccg agatcctcgc
     2641 gcccgccggg gaccgcgaca ccctggccgc ggcgctcgcc gccggcgcgg acgccgtgta
     2701 cttcgggctc gacgacggct tcaacgcgcg cgcccgcgcg gccaacttcg cgtcggccga
     2761 tctcggcgag gtgatcgcgc agatccaccg cgccggggtg cgcgcctgcg tcacgctcaa
     2821 cacgctggtg ttcgagcccg agctgccggt cgtcgaggcg ctgatccggc gggtcgccgc
     2881 cgcgggggcc gacgcgatca tcgtccagga cccggcggtc gccctgctcg cccgcgcgat
     2941 ctgccccgac ctcgagatcc atggctcgac ccagatgacc gcgtcgagcc cgctggcggc
     3001 cgagctgctg cgcggcctgg gcctcgcgcg cgtcgtggtg ccgcgcgagc tgtcggtcga
     3061 cgagatccgg gcgtacgccg ccggcacgtc gctgccgctc gaggtgttca tccacggcgc
     3121 gctgtgcgtg tcgtggagcg gccagtgcct gtcgtcggag gcctggggcg gccggtcggc
     3181 caaccgcggc cagtgcgcgc aggcctgccg cctgccctac cagctgatcg tcgacggccg
     3241 cgagcgcgac ctcggcgacg tcgcgtacct cctgtcgccc agggacctga ccgggctcga
     3301 ccacgtcgcg cggctcgccg agctcggcgt cgcggcgctc aagatcgagg gccgcctcaa
     3361 gggcccgcag tacgtcgcga ccaccgtcgc gcagtatcgc gccgcggtcg ccgccgccac
     3421 cggcgagtcg ccgcccacct cgccggtgcg gattcccagc gacgagccat cgctccacat
     3481 cgcctacagc cgcggcatct cgcgcggctt cctcgcaggc cccgaccacc agtcgctcat
     3541 cgagggccgc ttcc
//
LOCUS       DASVOR010002432         1538 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_115482,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002432 DASVOR010000000
VERSION     DASVOR010002432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115482"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1538)
                     /locus_tag="VF516_44650"
     CDS             complement(<1..>1538)
                     /locus_tag="VF516_44650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114925.1"
                     /translation="LSQQLGQCLALWLAARRLPPVGPLPAFSVDDLRDVADRLAMADE
                     LIGQGRELGIVPRALTQPPPRLPVPFLRVLAELSRDDARTIDPAILRIDPSHPVARRN
                     SYVTGLASGEVDRRAILPLTEEAPMYAKPHLSIWGEPFAADRPRENMGVRHQGIAASL
                     MPANPYACHNYSLQLAEVGRREESYRWADRATAAAPQFGAAHLDCVRRLRQVGRPGQA
                     FAEAQYRCREILDRAAAGKLSGNDWQAPHHAALLIALVHLDVGRLAEAIELADEVIAR
                     LPEDAATREAFGWATRRIAHWKADPGVLARAYAAEGHHRGDVGRVLAGLGRTRIADDE
                     DAMMLIEALCAIGRTDQAVTAYWQCAGLDVLGDGKARLAAARALILAGDLDEALDQIQ
                     IAQLRRGQSRLEAEINRVLRLAAIRPASDWEPVIARRLDRGAVALAQMAARDLCDFVP
                     GLDTEVTRRALGSRRRLAIDPVWIAELIAAVPAAQSSSPAIVARLAPPEHATLAAADA
                     LAAE"
BASE COUNT          191 a          572 c          588 g          187 t
ORIGIN      
        1 cactcggcgg cgagcgcgtc ggccgccgcg agcgtggcgt gctcgggtgg cgcgagccgg
       61 gcgacgattg ccggcgacga gctttgcgcc gcgggcaccg cagcgatcag ctcggcgatc
      121 cacaccggat cgatcgcgag ccggcgacgg cttcccagcg cgcgccgggt cacctcggtg
      181 tcgagcccgg gcacgaaatc gcacaggtcg cgcgcggcca tctgcgccag cgcaaccgca
      241 ccccgatcga gccggcgcgc gatcaccggc tcccagtcgc tcgccggccg gatcgcagcg
      301 agccgcagca cgcggttgat ctcggcctcg agccggctct ggccgcggcg gagctgcgcg
      361 atctggatct ggtcgagcgc ctcgtcgaga tcgccggcca ggatcagcgc ccgggcagcg
      421 gccagccgcg ccttgccatc gcccagcacg tcgagcccgg cgcactgcca gtaggcggtc
      481 accgcctggt cggtccggcc gatcgcgcac agcgcctcga tcagcatcat cgcgtcctcg
      541 tcgtcggcga tccgcgtccg gccgagcccg gcgagcacgc ggccgacgtc gccgcggtgg
      601 tggccctcgg ccgcgtaggc gcgcgcgagc accccaggat cggccttcca gtgcgcgatc
      661 cgccgggtcg cccagccgaa cgcctcgcgg gtcgcggcgt cctcgggcaa ccgggcgatg
      721 acctcgtcgg cgagctcgat cgcctcggcg agccggccga cgtcgaggtg gaccagcgcg
      781 atcagcagcg cggcgtggtg cggcgcctgc cagtcgttgc ccgacagctt gccggcggcc
      841 gcgcgatcga ggatctcgcg gcagcggtac tgcgcctcgg cgaacgcctg gccgggccgg
      901 ccgacctggc gcagccggcg cacgcagtcg aggtgcgcgg ccccgaactg cggtgcggcg
      961 gcggtcgccc ggtcggccca gcggtagctc tcctcgcgcc ggccgacctc ggcgagctgc
     1021 agcgagtagt tgtggcaggc gtacgggttg gccggcatca ggctggccgc gatgccctgg
     1081 tggcgcacgc ccatgttctc gcgcggccgg tcggccgcga acggctcgcc ccagatcgag
     1141 aggtggggct tggcgtacat cggcgcttcc tcggtgagcg gcaggatggc gcggcgatcg
     1201 acctcgccgc tggccagccc ggtgacgtag ctgttgcggc gcgccaccgg atggctcgga
     1261 tcgatccgga ggatcgccgg atcgatcgtc cgtgcgtcgt cgcgcgacag ctcggccagc
     1321 acgcgcagga acggcaccgg cagccgcggg ggcggctggg tcagcgcgcg ggggacgatg
     1381 ccgagctcgc ggccctgccc gatcagctcg tcggccatcg ccaggcggtc ggcgacatcg
     1441 cgcagatcgt cgaccgagaa cgcgggcagc gggccgacgg gcggcagccg gcgcgccgcc
     1501 agccacagcg cgaggcactg gccgagctgc tgcgacag
//
LOCUS       DASVOR010002433         2121 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_347544,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002433 DASVOR010000000
VERSION     DASVOR010002433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347544"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1401
                     /gene="ftsA"
                     /locus_tag="VF516_44655"
     CDS             172..1401
                     /gene="ftsA"
                     /locus_tag="VF516_44655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002634374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEX8114926.1"
                     /translation="MAKTKTSEIIVGLDIGTTKIACIAGEVTEDGVDIIGIGTAPSKG
                     LRRGYVVNIDATVSSIQQAVDEAENMAGCEISTVYAAISGAHVRGLNSHGIVAVKDKE
                     VRDVDVARVIEQAKAVAIPMDREVLHVLPQQYVVDDQDGIRDPLGMTAVRLEARVHIV
                     TTAVASAQNVIKCANRCNLQVADIVLESLASSQAVLEDDEKELGVALVDIGGGTCDVM
                     VYCDGAIVHTSVLALGGGHVTNDIATVLRTPLDSAEKIKKKYGCAWRNLIDDSEKMEV
                     PSVGGRGPRVMPRRELVAVIEPRIEEIFEHVKKELMRSGYFDSLAAGIVLTGGATAMD
                     GVGDVAEQVLGLPTRRGVPTKIGGLVDVVRSPAYSTGVGLVMFGADQGLSIQMPARGE
                     DRPGMFKRAWSRLAEMF"
     gene            1514..>2121
                     /locus_tag="VF516_44660"
     CDS             1514..>2121
                     /locus_tag="VF516_44660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEX8114927.1"
                     /translation="MALIEFDDVSHAKILVIGVGGGGGNAVNTMISGNLDGVEFVVAN
                     TDMQALEANMAPHKIQLGNALTKGLGAGANPEVGRRAAEESMQQIADLIAGADMVFVT
                     AGMGGGTGTGAAPVIAQIARDCGALTVGVVTKPFGFEGKKRARQAVEGIERLETAVDT
                     LIVIPNNRLLALVGTQTSMVEAFRKADSVLLNAVQGISDLMTV"
BASE COUNT          365 a          748 c          730 g          278 t
ORIGIN      
        1 gatcgcgatc cagctcggcc cgctgggcgg cgagctcgat gcgcgcatgc gcaccttcga
       61 tgccgcgtgg atcgcgctga gcgacgccga gcgcgctcgc acgcgcgcga tccacctcgg
      121 cgcccgcccc gaccacgtca ccgtcgcgtt cgccaccgac tagagagcac catggccaag
      181 accaagacca gcgagatcat cgtcggcctc gacatcggca ccaccaagat cgcgtgcatc
      241 gccggcgagg tcaccgagga cggcgtcgac atcatcggga tcggcaccgc gccgtccaag
      301 ggcttgcgcc ggggctacgt cgtcaacatc gacgccaccg tgtcgtcgat ccagcaggcg
      361 gtcgacgagg ccgagaacat ggccggctgc gagatctcga cggtctacgc cgcgatctcg
      421 ggcgcccacg tccgcggcct caactcgcac ggcatcgtcg cggtcaagga caaggaggtc
      481 cgcgacgtcg acgtcgcccg cgtgatcgag caggccaagg cggtcgcgat cccgatggac
      541 cgcgaggtgc tccacgtcct gccgcagcag tacgtcgtcg acgaccagga cggcatccgc
      601 gacccgctcg gcatgaccgc ggtccggctc gaggcccggg tccacatcgt caccaccgcg
      661 gtcgcctcgg cccagaacgt catcaagtgc gccaaccgct gcaacctcca ggtcgccgac
      721 atcgtgctcg agtcgctcgc gtcatcgcag gcggtgctcg aggacgacga gaaggagctc
      781 ggcgtcgcgc tggtcgacat cggcggcggg acctgcgacg tcatggtcta ctgcgacggc
      841 gcgatcgtcc acacctcggt gctcgcgctc ggcggcggcc acgtcaccaa cgacatcgcc
      901 accgtgctgc gcacgccgct cgactcggcc gagaagatca agaagaagta cggctgcgcg
      961 tggcgcaacc tgatcgacga cagcgagaag atggaggtcc cgtcggtcgg cggccgcggc
     1021 ccgcgcgtca tgccgcgccg cgagctggtc gcggtgatcg agccgcggat cgaggagatc
     1081 ttcgagcacg tcaagaagga gctgatgcgg tcgggctact tcgacagcct ggccgccggc
     1141 atcgtgctga ccggcggcgc gaccgcgatg gacggcgtcg gcgacgtcgc cgagcaggtg
     1201 ctcgggctgc cgacccggcg cggcgtgccg accaagatcg gcggcctggt cgacgtcgtg
     1261 cgctcgcccg cgtactcgac cggcgtcggc ctggtcatgt tcggcgccga ccagggcctg
     1321 agcatccaga tgcccgcccg cggcgaggac cggcccggga tgttcaagcg cgcgtggagc
     1381 cggctggcag agatgttctg aaccaggcgc gcgcaggcgc tcgtcggagg aatcgtccga
     1441 ccccgcgccg caaaaagttg cccgcacaga gcgcccactt tcatatgggc ctaccagggg
     1501 gaagcacaac accatggcac tcatcgaatt cgacgacgtc agtcacgcga agatcctcgt
     1561 catcggcgtc ggtggcgggg gcggcaacgc ggtcaacacc atgatcagcg gcaacctgga
     1621 cggggtcgag ttcgtggtcg cgaacaccga catgcaggcg ctggaagcca acatggcgcc
     1681 gcacaagatc cagctcggca acgcgctgac caagggcctg ggcgcgggcg ccaaccccga
     1741 ggtcggccgg cgcgccgccg aggagagcat gcagcagatc gccgacctga tcgcaggcgc
     1801 cgacatggtg ttcgtgaccg cggggatggg cggcggaacc ggcaccggcg cggccccggt
     1861 gatcgcccag atcgcccgcg actgcggggc gctcaccgtc ggcgtggtca ccaagccgtt
     1921 cggcttcgaa ggcaagaagc gcgcccgcca ggcggtcgag ggcatcgagc gcctcgagac
     1981 cgcggtcgac acgctgatcg tgatccccaa caaccggctg ctcgccctgg tcggcaccca
     2041 gacctcgatg gtcgaggcgt tccgcaaggc cgactcggtg ctgctcaacg ccgtgcaggg
     2101 catcagcgac ctgatgaccg t
//
LOCUS       DASVOR010002434         4015 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_28498,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002434 DASVOR010000000
VERSION     DASVOR010002434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4015
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28498"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..460)
                     /locus_tag="VF516_44665"
     CDS             complement(<1..460)
                     /locus_tag="VF516_44665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114928.1"
                     /translation="MHGTRYDRVSGRLVRVIDRTGACRVQLTWSDAADRLVSLGVDGA
                     IVDGAIRHHRLLGAAHAVGDTAMTALDWARPTEIPAIAAPGRLPLGAGAAILNTIALL
                     ARDAGVAALRYAGPYPTAALWRSLARSFRCDADEAAFTAGALDRAVRVARD"
     gene            536..2200
                     /locus_tag="VF516_44670"
     CDS             536..2200
                     /locus_tag="VF516_44670"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF006942.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEX8114929.1"
                     /translation="MKLGIVNETRPDERRVAASPAVVGRWVKGGWDVVVERGAGANAS
                     YPDAQYEAAGAAIVDRQTAWQADIVLKLRPPTVENGACEADQMRDGATLISYIQPAEN
                     PELVERLAKKQLTVLAMDQVPRISRAQKMDALSSMANIAGYRAVVEAAQRFGSFFTGQ
                     FTAAGKVPPAKVLIIGGGVAGLAALGAAKGLGAIVRAFDVRPSVEDQVKSLGGEFLTV
                     SLQESGEGQGGYAREMSKEFIEAEYALFRAQAKEVDIVITTALIPGKPAPKLWLTDMV
                     EAMKPGSVIVDLAAEKGGNCELTRPGEVIEHKGVTIIGYTDLASRMAHVASDMYGTNL
                     AHFLDEMGGAKGYKVDHDNDAVRPALVLEGGEKRGPPPPPAKKAEPLAQVPAAAKASG
                     PIPVVAKPASAAPVPIKPEAPRPSGHGGHGAPAGAPSRTSGVVMAVIGLVLAAVWLYL
                     RFGQGSVEAAASAPSVALLQHLTVFVMAVFVGFQVVWNVTPALHTPLMSVTNAISGII
                     VVGGLLGSQHGLNPSVGVALVATLFATINIAGGFLVTRRMLAMFRK"
     gene            2203..3606
                     /locus_tag="VF516_44675"
     CDS             2203..3606
                     /locus_tag="VF516_44675"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEX8114930.1"
                     /translation="MKTTELLSNAAYLVAGVLFILALRGLSSQETARRGNTYGIVGML
                     IAIGATLSLTGAATNPWVAAAIGVAAVVGATMALRVGMTAMPEMVALLHSFVGLAAVL
                     VGFALQLAPEKPELAERIEVFIDVWIGAITTTGSVLAFLKLRGSVSGKPLLLPARHLF
                     NLAMLVASIVLAVLYASAGATWSLEAGIAIAAVLGVHLVVAIGGADMPVVVSMLNSYS
                     GWTAAAAGFMLQNDLLIITGALVGSSGAILSYIMCRAMNRSIWNVIFGGFGAAPPKAA
                     SGGPAKPAGDVQEIDAVALAARLRASKSVIIVPGYGMAVARAQHAVRELTEVLRAAGV
                     TVRFAIHPVAGRLPGHMNVLLAEANVPYDIVLEMDEINADFPATDVAIVIGANDIVNP
                     AAEDDPSSPIAGMPVLQVWKSHLVIVNKRSRAAGYAGIDNPLFYKPVTRMLFGDAKDA
                     IDKVVAALKTDAAAKAS"
     gene            complement(3641..>4015)
                     /locus_tag="VF516_44680"
     CDS             complement(3641..>4015)
                     /locus_tag="VF516_44680"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEX8114931.1"
                     /translation="YNRLNRTGDRAAHAEIEAFRAAGRRLPPREGILVSTLEPCVMCL
                     GAAMETAIDTVIYGLRADDDGGTSRVQPPQSADDQMPRIVGGIRERDSRARFERWLAL
                     PDHNRSQEPFIRGVLGTHGHAA"
BASE COUNT          563 a         1405 c         1473 g          574 t
ORIGIN      
        1 gatcgcgggc gacgcggacc gcgcggtcga gcgccccggc ggtgaacgcg gcttcgtcgg
       61 cgtcgcaccg gaacgaccgg gcgaggctgc gccacagcgc ggcggtgggg taggggcccg
      121 cgtaacgcag ggcggcgacg ccggcgtccc gggcgagcag cgcgatcgtg ttgaggatcg
      181 cggcgccggc gccgagcggc agccgtcccg gcgcggcgat cgccgggatc tcggtcggtc
      241 gcgcccagtc gagcgccgtc atcgcggtgt cgccgacggc gtgagcggcg ccgagcagcc
      301 ggtggtggcg aatcgccccg tcgacgatcg caccgtcgac ccccaggctg accagtcggt
      361 cggccgcgtc gctccaggtc agctgaacgc gacacgcgcc ggtccggtcg atgacgcgta
      421 cgagtctacc ggagacgcgg tcataccgtg tgccatgcac tcctgaaacc taacagcttt
      481 catgcagata gctctttccc ttccgccggt ggtttggcat ggtgtgcccc cttccatgaa
      541 gctaggcatc gtcaacgaga ctcgacccga cgagcgccgg gtcgcggcgt ccccggcggt
      601 ggtcggccgg tgggtgaagg gtggctggga cgtcgtcgtc gagcgcggcg cgggcgcgaa
      661 cgccagctat cccgacgcgc agtacgaggc ggcgggcgcg gcgatcgtcg accgccagac
      721 cgcctggcag gccgacatcg tgctcaagct gcggccgccc accgtcgaga acggcgcctg
      781 cgaggccgac cagatgcgcg acggcgcgac gctgatctcg tacatccagc cggcggagaa
      841 ccccgagctc gtcgagcggc tggccaagaa gcagctcacc gtgctcgcga tggaccaggt
      901 gccgcggatc agccgcgccc agaagatgga cgcgctgtcg tcgatggcca acatcgccgg
      961 ctaccgcgcg gtggtcgagg ccgcgcagcg gttcggcagc ttcttcaccg ggcagttcac
     1021 cgcggcgggc aaggtcccgc cggccaaggt gctgatcatc ggcggcggcg tcgccgggct
     1081 cgccgcgctg ggcgccgcca agggcctggg cgcgatcgtc cgcgcgttcg acgtccggcc
     1141 cagcgtcgag gatcaggtca agtcgctggg cggcgagttc ctcaccgtct cgctccagga
     1201 gtccggcgag ggccagggcg gctacgccag ggagatgagc aaggagttca tcgaggccga
     1261 gtacgcgctg ttccgcgccc aggcgaagga ggtcgacatc gtcatcacga ccgcgctgat
     1321 ccccggcaag ccggcgccca agctgtggct gaccgacatg gtcgaggcga tgaagccggg
     1381 ctcggtgatc gtcgacctcg ccgccgagaa gggcggcaac tgcgagctga cccggcccgg
     1441 cgaggtgatc gagcacaagg gcgtgacgat catcggctac accgacctgg cgagccggat
     1501 ggcgcacgtc gccagcgaca tgtacggcac caacctggcg cacttcctcg acgagatggg
     1561 cggcgccaag ggctacaagg tcgaccacga caacgacgcg gtgcggcccg cgctggtgct
     1621 ggagggcggc gagaagcgcg ggcctccgcc gccgccggcc aagaaggccg aaccgctggc
     1681 acaggtcccg gcggcggcca aggcgtcagg gccgatcccg gtcgtcgcca agccggcctc
     1741 cgccgcgccg gttccgatca agccggaggc gccgcgaccg tcgggccacg gcggccacgg
     1801 ggccccggcc ggagcgccgt cgcggacctc gggtgtcgtg atggcggtga tcggcctcgt
     1861 gctggcggcc gtctggctgt acctgcggtt cggccagggc agcgtcgagg cggcggcgag
     1921 cgcgccgagc gtcgcgctgc tccagcacct cacggtgttc gtgatggcgg tgttcgtcgg
     1981 gttccaggtc gtgtggaacg tgaccccggc gctgcacacc ccgctgatga gcgtgaccaa
     2041 cgcgatcagc ggcatcatcg tcgtcggcgg tctgctcggc agccagcacg ggttgaaccc
     2101 ctcggtcggc gtcgccctcg tggccacgct gttcgccacc atcaacatcg ccggcgggtt
     2161 cctggtgacc cgccggatgc tcgcgatgtt caggaaatag cgatgaagac cacggagctc
     2221 ttgtcgaacg ccgcgtacct cgtggccggc gtgctgttca tcctcgcgct gcgtgggctg
     2281 tcgagccagg agaccgcgcg gcgcggcaac acctacggca tcgtcgggat gctgatcgcg
     2341 atcggcgcga cgctgtcgct gaccggcgcg gcgaccaacc cgtgggtcgc cgcggcgatc
     2401 ggggtcgccg cggtggtcgg cgcgaccatg gcgctgcggg tcggcatgac cgcgatgccc
     2461 gagatggtcg cgctgctcca cagcttcgtc ggtctcgccg cggtgctggt cgggttcgcg
     2521 ctgcagctcg ctcccgagaa gcccgagctc gccgagcgga tcgaggtgtt catcgacgtc
     2581 tggatcggcg cgatcaccac caccggctcg gtgctcgcgt tcctcaagct gcgcggctcg
     2641 gtcagcggca agccgctgct gctgccggcc cgccacctgt tcaacctcgc gatgctggtc
     2701 gcgtcgatcg tgctcgccgt gctgtacgcc agcgccggcg cgacctggtc gctcgaggcc
     2761 ggcatcgcga tcgccgccgt gctcggcgtc cacctggtcg tcgcgatcgg cggcgccgac
     2821 atgccggtcg tggtgtcgat gctcaacagc tactcgggat ggaccgcggc ggcggcgggc
     2881 ttcatgctgc agaacgacct cttgatcatc accggcgcgc tggtcggctc gagcggcgcg
     2941 atcctgtcgt acatcatgtg ccgcgcgatg aaccgctcga tctggaacgt gatcttcggc
     3001 gggttcggcg cggcgccgcc caaggccgcg tccggcgggc cggccaagcc cgcgggcgac
     3061 gtccaggaga tcgacgccgt cgccctcgcg gcgcggctgc gggcctcgaa gtcggtgatc
     3121 atcgtcccgg gctacggcat ggcggtcgcg cgcgcccagc acgcggtgcg cgagctgacc
     3181 gaggtgctgc gcgccgccgg ggtcaccgtg cggttcgcga tccacccggt cgccggccgc
     3241 ctgcccggcc acatgaacgt gctgctcgcc gaggccaacg tgccgtacga catcgtgctg
     3301 gagatggacg agatcaacgc cgacttcccg gccaccgacg tcgcgatcgt gatcggcgcc
     3361 aacgacatcg tcaacccggc cgccgaggac gatccgagct cgccgatcgc cggcatgccg
     3421 gtgctccagg tctggaagtc gcacctggtg atcgtcaaca agcggtcgcg cgcggcgggc
     3481 tacgccggca tcgacaaccc gctgttctac aagccggtga cccggatgct gttcggcgac
     3541 gccaaggacg cgatcgacaa ggtcgtcgcc gcgctcaaga ccgacgcggc ggccaaggcg
     3601 agctgatccg gcggatccgg cgccgcgatc gcgggtgcgg tcacgcggcg tgaccgtgag
     3661 tgcccaggac gccgcggatg aacggctcct ggctgcggtt gtggtcgggc agcgccagcc
     3721 agcgctcgaa ccgcgcccgg ctgtcgcgct cgcggatccc gcccacgatg cgcggcatct
     3781 ggtcgtcggc actctgcggc ggctggaccc ggctggtccc gccgtcgtcg tcggcacgca
     3841 ggccatagat cacggtgtcg atcgcggtct ccatcgccgc gccgaggcac atgacgcagg
     3901 gctcgagcgt cgagaccagg ataccttcac ggggcggcag ccggcgcccg gcggcccgga
     3961 acgcctcgat ctccgcgtgg gctgcgcgat cgccggtccg gttgaggcgg ttgta
//
LOCUS       DASVOR010002435         2542 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_172874,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002435 DASVOR010000000
VERSION     DASVOR010002435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2542
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172874"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(113..1165)
                     /locus_tag="VF516_44685"
     CDS             complement(113..1165)
                     /locus_tag="VF516_44685"
                     /inference="COORDINATES: protein motif:HMM:NF017686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0008218 - bioluminescence [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA reductase"
                     /protein_id="HEX8114932.1"
                     /translation="MTPDELADRLRRWVEQEPALPGPLDPATLAFCRRKLHQLAEPGL
                     LRRKLEREITGPWQPLGRVLLVISEHDVLGTVAGAVAACATGNRLRVKARTTRPLLED
                     LCRALELGRDDCEIRDWDGRDQDDAAVLDGIDAVLLAGSDALIRHYRTVAPPGVRLIE
                     YGPKIGVAAIGGEVDDRAALAGALASDVTLFAQGVCSSPQLIFIEDEGAARALRDRLL
                     AMELPPLPDAARLLQRVRLQELALLARLGDEILVDVAPRSGWGITISRDPAHRLPRGF
                     AFVVGPLADQLGAFARDHRRHLQTLGTWGRVPELGGFTHRCRIGRMHDRSPLAPHDGV
                     LELAQLVRLVSRGEAP"
     gene            complement(1162..2217)
                     /locus_tag="VF516_44690"
     CDS             complement(1162..2217)
                     /locus_tag="VF516_44690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114933.1"
                     /translation="MTSPISPIDQLLAAPDPYAALTPDDDARFAAAMAECDAHHRARN
                     PRYAQLWHGDERPVVPVGLFKRADLATPVDDEGVWLTSSGTGSGTATRVFFDRTSMER
                     IRRGMLQIFVHARFVDFQPSNFLLLSPDPASGAHPGYATTFEKFTGCAPVRERVFAVD
                     AAGRFQPEIALAALRRWAEGSAPVFLFGLTVFFEQLATCTAEPIRQRAHVRGLTGGGW
                     KGLAKQLERGQIIERLGALLSPPGLDIRDLFGLTEHPLHYISCPRGRFHLPKLARATI
                     LGETGEPAAPGEPGLIRLQCPLFASLPSHDLLTEDRGAWGDDCPCGEPLPYVAYLDRV
                     TPPAGTCAADASERAGG"
     gene            complement(2261..>2542)
                     /locus_tag="VF516_44695"
     CDS             complement(2261..>2542)
                     /locus_tag="VF516_44695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114934.1"
                     /translation="AVADPGGALVTEDAIRERHPELAGRALAPGDDHIPLAHLTSDPP
                     GPVIRSYDSIPHGERYLAIGTPKDHRALINYPPNLEALALDLAAVRAAE"
BASE COUNT          329 a          920 c          989 g          304 t
ORIGIN      
        1 ggcccagcgc ccgacgcgcg gtgcacgagc gccgtgacct cgtggcctgc gtcgcggagc
       61 cggcgcacga ggtgcccgcc gatgaagccg gtcgcgccgg tcgcgaacac cctcatggcg
      121 cctcgccgcg gctgaccagc cgcaccagct gtgcgagctc cagcacgccg tcgtgcgggg
      181 cgagcggcga gcgatcgtgc atgcgaccga tccggcagcg gtgcgtgaac ccgcccagct
      241 cggggacgcg gccccacgtg ccgagcgtct gcaggtggcg ccggtggtcg cgcgcgaacg
      301 cgccgagctg gtccgcgagc ggcccgacga cgaacgcgaa gcccctgggc agacggtgcg
      361 cgggatcgcg cgagatcgtg atcccccagc ccgagcgcgg cgcgacgtcg acgaggatct
      421 cgtcgccgag ccgcgcgagc agcgccagct cctggagccg cacccgctgg agcagccgcg
      481 cggcgtcggg gagcggcggg agctccatgg cgagcaggcg gtcgcggagc gcgcgcgcgg
      541 cgccctcgtc ctcgatgaag atcagctgcg gcgagctgca cacgccctgc gcgaacagcg
      601 tgacgtccga ggccagcgcg ccggcgaggg cggcccggtc gtcgacctcg ccgccgatcg
      661 ccgcgacgcc gatcttcggc ccgtactcga tcagccgcac gccgggcggc gcgacggtgc
      721 ggtagtgccg gatcagcgcg tcgctgccgg cgagcagcac ggcgtcgatc ccgtcgagca
      781 ccgcggcgtc gtcctgatcg cggccgtccc agtcgcggat ctcgcagtcg tcgcggccga
      841 gctcgagcgc gcgacacaga tcctcgagca gcgggcgcgt ggtgcgcgcc ttgacccgca
      901 gccggttgcc ggtggcacac gcggcgaccg cgccggccac ggtaccgagc acgtcgtgct
      961 cggagatcac gagcagcacg cgccccagcg gctgccacgg cccggtgatc tcgcgctcga
     1021 gcttgcgccg gagcaggccc ggctcggcga gctggtgcag cttgcggcgg cagaacgcca
     1081 gcgtcgcggg atcgagcggc ccggggagcg ccggctcctg ctccacccac cggcggaggc
     1141 gatcggccag ctcgtcgggc gtcatccgcc ggcgcgctcc gacgcgtccg ccgcgcaggt
     1201 ccccgccggc ggcgtgacgc gatcgaggta tgcgacgtac ggcagcggct cgccgcacgg
     1261 acagtcgtcg ccccacgcgc cgcggtcctc ggtgaggagg tcgtggctcg gcagcgacgc
     1321 gaacagcggg cactgcaggc ggatcaggcc gggctcgccg ggcgcggcgg gctcgccggt
     1381 ctcgccgagg atcgtcgcgc gcgcgagctt ggggaggtgg aaccggccgc gcgggcagct
     1441 gatgtagtgc agcgggtgct cggtcaggcc gaacaggtcg cggatgtcga gccccggcgg
     1501 cgaaagcagc gcgccgagcc gctcgatgat ctggccgcgc tcgagctgct tcgcgaggcc
     1561 cttccagccg ccgccggtga gcccgcggac gtgcgcgcgc tgccggatcg gctcggcggt
     1621 gcaggtggcg agctgctcga agaacacggt gagcccgaac aggaacaccg gcgcgctgcc
     1681 ctcggcccag cggcgcagcg ccgcgagcgc gatctcgggc tggaaccgcc cggcggcgtc
     1741 gaccgcgaac acccgctcgc gcaccggcgc gcagccggtg aacttctcga acgtcgtggc
     1801 gtagccgggg tgcgcgccgg acgccgggtc gggcgacagc agcaggaagt tcgacggctg
     1861 gaagtccacg aaccgcgcgt ggacgaagat ctgcagcatg ccgcgccgga tgcgctccat
     1921 gctcgtgcga tcgaagaaca cgcgcgtcgc ggtgccggat ccggtgcccg acgaggtcag
     1981 ccatacgccc tcgtcgtcga ccggcgtcgc gaggtcggcg cgcttgaaca ggccgaccgg
     2041 gaccaccggc cgctcgtcgc cgtgccagag ctgtgcgtag cgcgggttgc gcgcgcggtg
     2101 atgcgcgtcg cattcggcca tcgcggccgc gaaccgcgca tcgtcgtcgg gagtgagcgc
     2161 cgcgtacggg tcgggcgcgg cgagcagctg gtcgatcggc gagatcggag aggtcatggt
     2221 gcctccgctt ccgtccggcg cctgccgctc cggacctcgg tcattctgcc gccctcaccg
     2281 ccgcgaggtc cagcgcgagc gcctcgaggt tcggcgggta gttgatcagc gcgcggtggt
     2341 ccttcggcgt gccgatcgcc aggtagcgct cgccgtgcgg gatcgagtcg tagctgcgga
     2401 tcaccggccc cggcggatcg gacgtcaggt gagcgagcgg gatgtgatcg tcgcccggcg
     2461 cgagcgcccg gccggcgagc tcggggtggc gctcgcggat cgcgtcctcg gtgacgagcg
     2521 cgccgccggg gtcggccacc gc
//
LOCUS       DASVOR010002436         3382 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_435230,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002436 DASVOR010000000
VERSION     DASVOR010002436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435230"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..1712
                     /locus_tag="VF516_44700"
     CDS             195..1712
                     /locus_tag="VF516_44700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114935.1"
                     /translation="MARAAAAPRAVQRFVDTDLDRLLTERAGGDPMPAMLDLFHRTAA
                     GVPAYAAFLRERGIDPASVRGADDWRRLPLMTKPDYFRRHPLPALCRGGELAACDMVA
                     VSSGSTGEPTLWPRALADELAVASRFEQVFRDSFHADQRTTLAVVCFPLGTWVGGMFT
                     AAACRHLAAKGYPITVVTPGNHRAEILRVVAALGRQFDQTVLLGYPPFLKDVIDAGIV
                     DGFAWRDQPIRLVLAGEVISEEWRDLMAERLGLTDPARDTASLYGTADAGVLGNETPL
                     SIAIRRFLARDPAAARAVFGQARLPTLVQIDPFERFFEEHDGTLVFSADGGIPLVRYH
                     IADEGGIWPHAALIARLAELGFDAEACARRGGDRGIRPLPFVFVFGRSHFAVSYYGAN
                     VFPDTVIIGLEQPAIRELVTGKFVLEVREDDDRNRALWIAVELSPAGAHAPPGPLGDA
                     IAEAILASLGSRSSEFANYVPAARQRPRITLWPTGHPEYFPVGVKHRYSRPPASR"
     gene            complement(1804..>3382)
                     /locus_tag="VF516_44705"
     CDS             complement(1804..>3382)
                     /locus_tag="VF516_44705"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEX8114936.1"
                     /translation="DEPSAAEDDARVPRTLLSYVVRTQESGVFDAAGDAGRFANDPYF
                     TDTRPRSVLCMPVRLRADSVALLYLENELVPGTFTPERLLALELLAAQAAISLENAQL
                     LERERTRRIEAEAAERRGRLLGEATALLSQTLDSRSVLDALARLFTRWFADMAVIDLQ
                     DNGALVRIACAHRDPDKEPLLREAAMRYPAQPRSPVWQVLQTGKAIEVPILTDEQIGA
                     YCVDDHQAEFARQLGARCGVFVPLRARDAVIGVLSLVSMTPSRFARADVEFAVDLGRR
                     MALAIDNAWLLDKTRRALHLRDEFLRIASHELRTPLASLRLSAQGLLRAAERSQTVSP
                     EILDRTLRRVLGNTIRLEQLTSELLNVTRIEQGRLDLNPIEIALDAIVRGAVEHIEAD
                     LTTAGSSVSIECAVPVVGRWDPSRIDQVVTNLLTNAAKFGAGKPIEIRIERIGDTARL
                     TVTDHGIGIDPARRPYVFDRFERAVPSSRYGGLGLGLYIARSIVAAHGGTITVDSELG
                     AGSMFTVTLPCSTPEPG"
BASE COUNT          466 a         1238 c         1182 g          496 t
ORIGIN      
        1 cgatcgcatg cgcgcccagg cggcgggctt cgatcaccac ctgatcaagc cgatcacggc
       61 cgaggcgctg atcacgctgc tcgcggccga gccacccgcc gctcgcgaag ggtagactcc
      121 gcgaggcccg cgaggccccg atgccacccc ccgacggcag gatcgttccg gacgcttcat
      181 cccacgcggc acgcttggcg cgcgcggcgg ccgctccgcg cgccgtgcag cgcttcgtcg
      241 acaccgatct ggatcggctg ctcaccgagc gcgccggcgg cgatccgatg ccggcgatgc
      301 tcgacctgtt ccatcgcacc gcggccggcg tgccggcgta cgccgcgttc ctgcgcgagc
      361 gcgggatcga tccggcctcg gtgcgcggcg ccgacgactg gcgccgcctg ccgctgatga
      421 ccaagcccga ctacttccgc cgccacccgc tgcccgcgct gtgccgcggc ggcgagctcg
      481 cggcgtgcga catggtcgcc gtgtcgtcgg gatcgaccgg cgagccgacc ttgtggccgc
      541 gcgcgctcgc cgacgagctc gcggtggcga gccggttcga gcaggtgttc cgcgacagct
      601 tccatgccga ccagcgcacc accctggcgg tggtgtgctt tccgctcggc acctgggtcg
      661 gcgggatgtt caccgccgcc gcctgccgcc acctcgcggc caagggctac ccgatcaccg
      721 tggtcacgcc gggcaatcac cgggccgaga tcctgcgcgt cgtggccgcg ctcggccggc
      781 agttcgacca gaccgtgctg ctcggctacc cgccgttcct caaggacgtg atcgacgcgg
      841 gcatcgtcga cggcttcgcc tggcgcgacc agccgatccg cctggtgctc gccggcgagg
      901 tgatcagcga ggagtggcgc gatctgatgg ccgagcggct cggcctgacc gatccggcgc
      961 gcgacaccgc gtcgctgtac ggcaccgccg acgccggcgt gctcggcaac gagaccccgc
     1021 tgtcgatcgc gatccggcgg ttcctcgccc gcgatccggc cgccgcgcgc gcggtgttcg
     1081 gccaggcccg cctgccgacc ctggtccaga tcgacccgtt cgagcggttc ttcgaggagc
     1141 acgacggcac gctggtgttc tccgccgacg gcggcatccc gctggtccgc taccacatcg
     1201 ccgacgaggg cgggatctgg ccgcacgcgg cgctgatcgc gcggctcgcc gagctcgggt
     1261 tcgacgccga ggcctgtgcc cggcgcggcg gcgaccgcgg gatccggccg ctgccgttcg
     1321 tcttcgtgtt cggccgctcg cacttcgcgg tgtcgtacta cggcgccaac gtgtttcccg
     1381 acaccgtgat catcggcctg gagcagccgg cgatccgcga gctggtcacc ggcaagttcg
     1441 tcctggaggt ccgcgaggac gacgaccgca accgcgcgct gtggatcgcg gtcgagctgt
     1501 cgcccgccgg tgcgcacgcc ccgccggggc cgctcggcga cgcgatcgcc gaggcgatcc
     1561 tcgccagcct ggggagccga agcagcgagt ttgcgaacta cgtcccggcc gcgcgccagc
     1621 ggccgcggat cacgctgtgg ccgaccggtc accccgagta ctttcccgtc ggggtcaagc
     1681 accgctactc gcggccgccg gcgagccggt gacctggccc ggagcatcca ccaggtggat
     1741 cgccaccttg accgttcagc aaagtgaacg gcttagcgct acaaatcgca ccgttgctgc
     1801 tggttacccc ggttcgggtg tggagcacgg cagggtcacg gtgaacatgg agccggcgcc
     1861 cagctcgctg tcgacggtga tcgtgccgcc gtgcgcggcg acgatcgacc gggcgatgta
     1921 caaccccagc ccgagcccgc cgtagcgcga ggacgggacc gcgcgctcga atcgatcgaa
     1981 cacgtagggg cggcgcgccg ggtcgatgcc gatgccgtgg tcggtcacgg tcagccgcgc
     2041 ggtgtcgcca atccgctcga tccggatctc gatcggtttg ccggcgccga acttggccgc
     2101 attggtcagc aggttcgtca ccacctgatc gatccgcgac ggatcccatc gtcccacgac
     2161 cggcaccgcg cactcgatcg acacggaaga tcccgccgtg gtgagatcgg cctcgatgtg
     2221 ctccaccgcg ccgcgcacga tcgcgtcgag ggcgatctcg atcggattca gatcgaggcg
     2281 gccttgctcg atgcgggtga cgttgagaag ctccgacgtg agctgctcga gacggatggt
     2341 gttgccgagc acgcggcgca gcgtgcggtc caggatctcc ggcgacaccg tttgactgcg
     2401 ctcggcggcg cgcagcagcc cctgcgcgct gagccggagc gacgcgagcg gcgtgcggag
     2461 ctcgtgcgac gcgatccgca ggaactcgtc gcgcaggtgc agcgcgcgcc gcgtcttgtc
     2521 gagcagccac gcgttgtcga tcgccagcgc catccggcgg ccgagatcga ccgcgaactc
     2581 gacgtcggct cgtgcgaacc ggctcggggt catcgagacc agcgacagca cgccgatcac
     2641 cgcgtcccgg gcgcgcagcg gcacgaacac tccacatcgc gcgccgagct ggcgggcgaa
     2701 ctcggcttgg tggtcgtcga cacagtaggc cccgatctgt tcgtcggtca ggatgggaac
     2761 ctcgatcgcc ttgccggtct gcaggacttg ccacaccggg gacctgggtt gcgccgggta
     2821 gcgcatcgct gcctcgcgga ggagcggctc cttgtcggga tcgcggtgcg cgcaagcgat
     2881 ccgcaccagc gcgccgttgt cctggagatc gatcaccgcc atgtccgcga accatcgcgt
     2941 gaacagccgg gccagtgcgt cgagcacgct ccgggagtcg agcgtctgtg acagcagcgc
     3001 ggtcgcttcg ccgagcagcc gcccgcggcg ctcggccgcc tccgcctcga tccgcctggt
     3061 gcgctcgcgc tcaagcagct gcgcattctc gagcgagatc gccgcctgcg ctgcgaggag
     3121 ctcgagcgcg agcagacgct ccggggtgaa cgtgcccggg accagctcgt tctcgaggta
     3181 gagcagcgcg accgaatccg cccgcaggcg aacaggcatg cacagcaccg agcgcgggcg
     3241 ggtgtcggtg aagtacggat cgttcgcgaa ccgtccagcg tcgccggcgg cgtcgaacac
     3301 ccccgactcc tgggtccgca ccacgtacga cagcagcgtc ctcgggacgc gcgcgtcatc
     3361 ctccgctgcg gagggctcgt cg
//
LOCUS       DASVOR010002437         2796 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_553545,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002437 DASVOR010000000
VERSION     DASVOR010002437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553545"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1584)
                     /gene="glgC"
                     /locus_tag="VF516_44710"
     CDS             complement(172..1584)
                     /gene="glgC"
                     /locus_tag="VF516_44710"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:NF001947.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEX8114937.1"
                     /translation="MAALVKNTLVMIMAGGKGSRLGPLTVHRSKPGVPFAGRYRIIDF
                     VLSNFVNSGYRRIYVLTQYMSSSLIKHLSRNWRLSGFGEYIEVVPAQMRLGEFWYRGT
                     ADAVYQNLNLVRDARAEHVCVFGGDHVYKFAVDQMEIFHRDHDADLTVAAFPVPVHEA
                     SAFGIIDVDDRGQIINFIEKPKQPPEMPGRPGWSLVSMGNYIFRRDVLERVLVEDGGQ
                     PSSRHDFGRDIIPRLVSQGARVFVYDFAQNQIPGEPEDATPYWRDVGTIDSYFTSNME
                     LRARVPLLDLYNRQWRIRSAQRDYPPARFVRAGEGFGPAEVDDSLICEGSIIASAAVR
                     ESVLSYDCFVHAGAELCDSVILSGCDIGRNARLRRVLLDKNCKVEPGCVIGDDPRHDR
                     ERFPFVTDSGIVVIPKGTLVPAKGPFVLANDIAELIGNEPDLLGQLRPGTFVVSTQNR
                     HSYESAAGPRFKKYAGGEVG"
     gene            complement(1686..2255)
                     /gene="orn"
                     /locus_tag="VF516_44715"
     CDS             complement(1686..2255)
                     /gene="orn"
                     /locus_tag="VF516_44715"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEX8114938.1"
                     /translation="MAGSDVLVWLDMEMTGLDPERERIIEIATILTDGQLTEIAIGPE
                     LVIHQPDEILAAMDDWNTRHHGASGLTERVRQSQITDADAEAQTIAFIDAHVAPRDRP
                     VLAGNSIHQDRRFIRRYMPALEKRLHYRMVDVSTIKELARRWYPQIIAKQPAKRDTHR
                     ALDDIRESIEELRFYRGQVFIAPPTPAAS"
     gene            complement(2279..>2796)
                     /locus_tag="VF516_44720"
     CDS             complement(2279..>2796)
                     /locus_tag="VF516_44720"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HEX8114939.1"
                     /translation="APRLAKKDAAAAAKSTRHGPPPAPGTRPARPVNLYNFWTHEWLA
                     IDAASPPPPATVDHFLRDHFTNKTTAMEPRLIGLLVAAATRFHSDVVDVVSGFRHPKY
                     NLILRKKGHQVARDSQHTHGNAIDFFVPQVPVQTLQAWARAQSAGGVGLYPESGFVHM
                     DTGPIRSWSGE"
BASE COUNT          401 a          908 c         1043 g          444 t
ORIGIN      
        1 gtacagcggg accagcaccg cggtggccag gccgtggccg gcgagcgccg cgggcaggcc
       61 ggcgagcacg tcgcccagcc cgccggactg ggcgaacggg gcgacctcgc tggcaacgtg
      121 gagtacgcgc acgtcgtgga cttctgcttc tacttgtacc gcggttgtcc gctagccgac
      181 ctcgccgccc gcgtacttct tgaaccgcgg gccggcggcc gactcgtagc tgtggcggtt
      241 ctgggtcgag accacgaacg tgccgggccg cagctggccg agcaggtcgg gctcgttgcc
      301 gatcagctcg gcgatgtcgt tggccagcac gaacggcccc ttggccggca ccagggtgcc
      361 cttggggatg acgacgatgc ccgagtcggt gacgaacggg aaccgctcgc gatcgtggcg
      421 cgggtcgtcg ccgatcacgc agcccggctc gaccttgcag ttcttgtcga gcagcacgcg
      481 gcgcagccgg gcgttgcggc cgatgtcgca gcccgacagg atcaccgagt cgcacagctc
      541 ggcgccggcg tggacgaagc agtcatagga cagcaccgac tcgcggaccg cggccgacgc
      601 gatgatcgag ccctcgcaga tcaggctgtc gtcgacctcg gccgggccga agccctcgcc
      661 ggcgcgcacg aaccgggccg ggggatagtc gcgctgcgcg ctgcggatcc gccactgccg
      721 gttgtagagg tcgagcagcg ggacccgggc gcgcagctcc atgttcgacg tgaagtagct
      781 gtcgatcgta ccgacgtcgc gccagtacgg cgtggcgtcc tcgggctcgc ccgggatctg
      841 gttctgcgcg aagtcgtaga cgaacacccg cgcgccctgc gagaccagcc gcgggatgat
      901 gtcgcggccg aagtcgtggc gcgagctcgg ctggccgccg tcctcgacca gcacgcgctc
      961 cagcacgtcg cgccggaaga tgtagttgcc catcgacacc agcgaccagc cggggcggcc
     1021 gggcatctcg ggcggctgct tgggcttctc gatgaagttg atgatctggc cgcggtcgtc
     1081 gacgtcgatg atgccgaacg cgctggcctc gtgcaccggc accgggaacg cggcgaccgt
     1141 caggtcggcg tcgtggtcgc ggtggaagat ctccatctgg tcgaccgcga acttgtagac
     1201 gtgatcgccg ccgaacacgc agacgtgctc ggcgcgcgcg tcgcgcacca ggttgaggtt
     1261 ctggtacacg gcgtcggcgg tgccgcggta ccagaactcg ccgagccgca tctgcgccgg
     1321 gacgacctcg atgtactcgc cgaaccccga cagccgccag ttgcgcgaca ggtgcttgat
     1381 caggctcgac gacatgtact gcgtgagcac gtagatccgg cggtagccgg agttgacgaa
     1441 gttcgacagg acgaagtcga tgatccggta gcgcccggcg aacggcacgc cgggcttgga
     1501 ccggtgcacg gtcagcgggc cgaggcgcga gcccttgccg cccgccatga tcatcacgag
     1561 ggtgttcttc acgagggcgg ccatccggct gacgcgatca tcctagcaag acgagcgccg
     1621 gatccagcgc tggcggccac gctcacgggg ttttgtgcat gaccggagcg gtggggccgc
     1681 ggcgctcagg acgcggccgg ggtcggcggg gcgatgaaca cctggccgcg gtagaaccgg
     1741 agctcctcga tcgactcgcg gatgtcatcc agggcccgat gggtgtcgcg cttggccggc
     1801 tgcttggcga tgatctgcgg gtaccagcgc cgcgcgagct ccttgatcgt cgagacgtcg
     1861 accatccggt agtgcaggcg cttctcgagc gccggcatgt agcgccggat gaaccggcga
     1921 tcctggtgga tcgagttgcc ggcgagcacc ggccggtcgc gtggcgcgac gtgggcgtcg
     1981 atgaacgcga tggtctgggc ctcggcgtcg gcgtcggtga tctgcgactg gcggacgcgc
     2041 tcggtcaggc ccgacgcgcc gtggtgcctg gtgttccagt cgtccatcgc ggcgaggatc
     2101 tcgtcgggct ggtggatcac gagctcgggg ccgatcgcga tctcggtgag ctggccgtcg
     2161 gtgaggatcg tcgcgatctc gatgatgcgc tcgcgttcgg ggtcgagccc ggtcatctcc
     2221 atgtcgagcc agaccaggac gtcgctgcct gccattgccc cactctacaa cagggcggct
     2281 actcgccgga ccagctgcgg atcggtccgg tgtccatgtg cacgaagccg ctttcggggt
     2341 agagcccgac accgccggcg ctctgcgccc gggcccaggc ctggagcgtc tgcacgggga
     2401 cctggggtac gaagaaatcg atcgcgttgc cgtgggtgtg ctggctgtcg cgcgcgacct
     2461 ggtggccctt cttgcgcagg atcaggttgt acttggggtg gcgaaacccc gagaccacgt
     2521 cgaccacgtc gctgtggaac cgggtcgccg cggcgaccag caggccgatc agccgcggct
     2581 ccatcgcggt cgtcttgttg gtgaagtgat cgcgcaggaa gtggtcgacc gtggccggcg
     2641 gcgggggcga cgcggcgtcg atcgccagcc attcgtgggt ccagaagttg tacaggttga
     2701 cgggtcgggc cggccgggtg ccgggcgcgg ggggcggccc gtgccgggtc gacttggcgg
     2761 cggccgcggc gtccttcttg gcgagccgcg gggcat
//
LOCUS       DASVOR010002438         3926 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_347629,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002438 DASVOR010000000
VERSION     DASVOR010002438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_347629"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1396
                     /locus_tag="VF516_44725"
     CDS             <1..1396
                     /locus_tag="VF516_44725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114940.1"
                     /translation="CASCYWGVALVLGPNYNVPMLANRFPAAWQALQRAQQLAPSATP
                     VEQALIAALARRYPGARPRAPDAMAPFNQAYADAMTEVAGQFPDDLDVQTLRAEALMD
                     LAPWKLWTADGKPAQHTVEILMTLESVLARDPRHPGANHYYLHAVEASPQPGRALASA
                     DRLPALMPGAGHIVHMPAHIYQRVGRYADASAANRRAAEIDQRYLPGAPAWGYYGMYL
                     SHNCGFLSYSAAMEGRSAESLAAARCAARTFPAVMLAMMPGMDFFVAEPLLAMVRFGK
                     FDGILATPRPDPGYPTLTALWLHAHGLARAATGDLAAADADLAELRRMAAQTAPGAVT
                     LTNEVRDILDVGTRTLEAVLAHKRGDPRELALWDAAVAAEDRLSYSEPSDWFYPVRHY
                     QGEALLELGRWSDAEAVYRADLANHPRNGWALWGLVRALRGQHRDREAAAVDQEFHRA
                     WANADFALTATAFW"
     gene            complement(1423..2202)
                     /locus_tag="VF516_44730"
     CDS             complement(1423..2202)
                     /locus_tag="VF516_44730"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HEX8114941.1"
                     /translation="MDVDLGEPGRERRVERLRHAHRGARAPAGVDAGVGRRAADRAQP
                     PELRRRYPELRLEIDLSDRNVPLVQGGFDLAIRVGRLSDSSLRAQLLGRVPVRLVASP
                     SYVAAHGEPARPLALRDHACLTIAQVIGPVEWSFYHHRTRTRSERLEIEGVVHTTSPL
                     LAAQLACAGQGILRVNEWVIRDQLARGELVEILRDWSCYRLAQGGAPVYVVYAQTASA
                     APPLKSRVFVQLLKEILAAEVLSRTASQAGRPAPPRGRAPR"
     gene            2083..2895
                     /locus_tag="VF516_44735"
     CDS             2083..2895
                     /locus_tag="VF516_44735"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEX8114942.1"
                     /translation="MSRSPTDTGIDTRRCPRPAMGVPQAFYAPFATWLAEVDVHALTF
                     DYRGTGRSRRRPLREVEADILTWARLDTTAALRALQDLAPDVPLTWIGHSLGAQIVPF
                     VPDHRELAKVITIAAGSGYWRDNVPALRPKVWLLWYVLTPLATPLFGYFPGKRLRMIA
                     DLPAGVVRQWRRWCLDPHYAVGAEGPVVRELYARVTAPITSLSFTDDEMMSERNIESL
                     HGFYVAAPTTMRRIAPAAVGMPRIGHFGFFRPASREPLWDAVLRPELALRRG"
     gene            complement(2892..>3926)
                     /locus_tag="VF516_44740"
     CDS             complement(2892..>3926)
                     /locus_tag="VF516_44740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114943.1"
                     /translation="LAALQADTIGDNARNGVDDADPDDGGWDFIIAPTATEHSTAASP
                     TNLFGAIALGEWAAVDSGSAGNRALVAALDAGLGMQRNPDVDSAPDFVFGVLLAELAD
                     NPGFADIARAHYDAKRASKTDAAGLGAFVRDARHAANEDGLIPYDVGWFVLAAAALDS
                     AFPHAGYDRDASTYAGIVVDDLTSATPDFDFRNPAEGFYGTGLAWSLVASAWLHDTRL
                     FRDVRAQLLAQQHGDGAWGSSAAHPGDDDDLQVTAHALQTLALTDPFTAQTRQAVRRA
                     ARFLLQGQAASGGWVDATHHELPLVDADIALGLMLSRTEAGEDGLVPDAPLSTRAPTA
                     PAPASAAPLP"
BASE COUNT          498 a         1450 c         1452 g          526 t
ORIGIN      
        1 ctgcgcgagc tgctactggg gcgtcgcgct cgtgctcggc ccgaactaca acgtgccgat
       61 gctcgccaac cggttccccg cggcgtggca ggcgctgcag cgcgcgcagc agctggcgcc
      121 ctcggcgacg cccgtcgagc aggcgctgat cgccgcgctc gccaggcgct atcccggcgc
      181 gcggccgcgt gcgcccgacg cgatggcgcc gttcaaccag gcctacgccg acgcgatgac
      241 cgaggtcgca ggccagttcc ccgacgatct cgacgtccag accctgcgcg ccgaggcgct
      301 gatggacctc gcgccgtgga agctgtggac cgccgacggc aagccggcgc agcacaccgt
      361 cgagatcctc atgacgctcg agagcgtgct cgcccgcgat ccgaggcacc cgggcgcgaa
      421 ccactactac ctccacgccg tcgaggcgtc accgcagccc gggcgcgcgc tcgcgtccgc
      481 cgaccggctg cccgcgctga tgcccggcgc cggacacatc gtgcacatgc cggcgcacat
      541 ctatcagcgc gtcggccgct acgccgacgc ctcggccgcg aaccgccggg cggccgagat
      601 cgatcagcgc tacctgccgg gcgcgccggc gtggggctac tacggcatgt acctctcgca
      661 caactgcggg ttcctgtcgt actcggcggc gatggagggc cgcagcgcgg agtcgctcgc
      721 ggccgcccgg tgtgccgcgc gcacgttccc ggcggtgatg ctcgcgatga tgccgggcat
      781 ggacttcttc gtggccgagc cgctgctcgc gatggtccgg ttcggcaagt tcgacggcat
      841 cctcgcgacg ccgcgcccgg atccggggta tccgaccctc acggcgctgt ggctgcacgc
      901 gcacggcctc gcgcgggcgg ccaccggcga cctggcggcg gcagacgccg atctcgcgga
      961 gctgcgccgg atggccgcgc agaccgcgcc gggcgcggtg accctgacca acgaggtccg
     1021 cgacatcctc gacgtcggca cccggacgct ggaggccgtc ctcgcccaca agcgcggcga
     1081 cccgcgcgag ctcgcgctgt gggacgcggc ggtcgccgcc gaggatcgcc tgtcgtattc
     1141 cgagccgagc gactggttct atccggtgcg ccactatcaa ggcgaggcgc tgctcgagct
     1201 cgggcggtgg agcgacgccg aggcggtcta ccgcgcggac ctcgcgaacc acccgcgcaa
     1261 cggctgggcg ctgtggggcc tggtgcgcgc gctgcgcggc cagcatcgcg atcgcgaggc
     1321 cgctgcggtc gatcaggagt tccaccgcgc gtgggcgaac gcagatttcg cgctgaccgc
     1381 caccgcgttc tggtgaccgc ggcggacgat gctccccgag gttcatcgag gcgcacggcc
     1441 gcgcggcggc gccggtcgcc ccgcttgcga ggcggttcgt gacagcacct cggcggcgag
     1501 gatctccttg agcagctgca cgaacacgcg cgacttgagc ggcggagcgg cgctcgcggt
     1561 ctgcgcgtac accacgtaca ccggcgcgcc gccctgggcg agccggtagc acgaccagtc
     1621 gcgcaggatc tcgaccagct cgccgcgcgc gagctggtcg cggatcaccc actcgttgac
     1681 ccgcaggatc ccctgtcccg cgcatgccag ctgcgcggcg agcagcgggc tggtggtgtg
     1741 gaccacgccc tcgatctcga gccgctcgct ccgggtgcgc gtgcggtggt ggtagaagct
     1801 ccactcgacc gggccgatca cctgcgcgat cgtgaggcac gcgtgatcgc gcagcgcgag
     1861 cggccgggcg ggctcgccgt gcgccgcgac gtagctcggg ctcgcgacca gccgcaccgg
     1921 aacccggccg agcagctgcg cgcgcagcga cgagtcggac agccggccga cgcggatcgc
     1981 gaggtcgaac ccgccctgga ccagcggcac gttgcgatcg gacagatcga tctcgagccg
     2041 cagctcggga tagcggcggc gcagctccgg cggttgggcg cgatcagccg ctcgccgacc
     2101 gacaccggca tcgacacccg caggtgcccg cgccccgcga tgggcgtgcc gcaggcgttc
     2161 tacgcgccgt tcgcgacctg gctcgccgag gtcgacgtcc acgcgctgac cttcgactac
     2221 cgcggcaccg ggcggtcgcg ccggcgcccg ctgcgcgagg tcgaggcgga catcctcacg
     2281 tgggcgcggc tcgacaccac cgcggcgctg cgcgcgctgc aggatctcgc cccggacgta
     2341 ccgctgacct ggatcggcca cagcctcggc gcgcagatcg tcccgttcgt gcccgatcac
     2401 cgcgagctcg ccaaggtgat cacgatcgcc gccggcagcg gctactggcg agacaacgtg
     2461 ccggcgctgc gccccaaggt gtggctcttg tggtacgtgc tgacgccgct ggccacgccg
     2521 ctgttcggct acttccccgg caagcggctg cgcatgatcg ccgatctgcc ggccggcgtg
     2581 gtccgtcagt ggcggcggtg gtgcctcgat ccgcactacg cggtgggcgc cgagggcccg
     2641 gtcgtgcgcg agctctacgc gcgtgtgacc gcgccgatca cctcgctgtc attcaccgac
     2701 gacgagatga tgtcggagcg caacatcgag tcgctgcatg ggttctacgt cgccgcgccg
     2761 acgacgatgc gccggatcgc gccggccgcc gtcggcatgc cgcggatcgg ccacttcggg
     2821 ttcttccgcc cggcctcgcg cgagccgctg tgggacgccg tgctgcgacc cgagcttgcg
     2881 ctgcgccgag gctagggcag gggcgcggcg ctcgccggag ccggcgcggt cggtgcgcgc
     2941 gtcgagagcg gcgcatccgg gaccaggccg tcctcgccgg cctcggtccg cgacagcatc
     3001 aggccgagcg cgatgtcggc gtcgaccagc ggcagctcgt ggtgggtcgc gtcgacccag
     3061 ccgcccgacg ccgcctggcc ctggagcagg aagcgcgcgg cgcggcgcac cgcctgccgg
     3121 gtctgcgcgg tgaacgggtc ggtcagcgcc agggtctgca gcgcgtgcgc cgtgacctgg
     3181 aggtcgtcgt cgtcgccggg gtgcgccgcg ctggaccccc acgcgccatc gccgtgctgc
     3241 tgggcgagca gctgcgcgcg cacgtcgcgg aacagccggg tgtcgtgcag ccacgcgctg
     3301 gcgaccagcg accacgccag cccggtgccg tagaacccct cggcggggtt gcggaagtcg
     3361 aagtccggtg tcgccgaggt cagatcgtcg acgacgatcc cggcgtaggt gctggcgtcg
     3421 cggtcgtagc cggcgtgggg gaacgccgag tcgagcgccg cggcggcgag cacgaaccag
     3481 cccacgtcgt acgggatcag gccgtcctcg ttggccgcgt ggcgcgcgtc gcggacgaac
     3541 gcgccgagcc cggcggcgtc ggtcttcgat gcgcgcttgg cgtcgtagtg cgcgcgcgcg
     3601 atgtcggcga acccagggtt gtcggcgagc tcggcgagca gcacgccgaa cacgaagtcg
     3661 ggcgccgagt cgacgtcggg gttgcgctgc atgccgagcc cggcgtcgag cgcggcgacc
     3721 agcgcgcggt tgccggcgct gccgctatcg accgccgccc actcgccgag cgcgatcgcg
     3781 ccgaacaggt tggtcgggct cgccgcggtc gagtgctcgg tcgcggtcgg cgcgatgatg
     3841 aagtcccagc caccgtcgtc gggatcggcg tcgtccacgc cgttgcgcgc gttgtcgccg
     3901 atcgtgtcgg cctggagcgc cgccag
//
LOCUS       DASVOR010002439         7707 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_523207,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002439 DASVOR010000000
VERSION     DASVOR010002439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7707)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7707)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7707
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523207"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="VF516_44745"
     CDS             <1..610
                     /locus_tag="VF516_44745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114944.1"
                     /translation="SIARGHLPLGFLHDSDGHFRGTLMGVGHDFTVTCKDAAGTVQTV
                     CNSMTDSATVTVKVTGNLQTTNFSSMIDREGNFTITGLQGATATFNGDSSFSLDTSLT
                     SVFHPGVTSTFTFDATASYKAITIATADHTITGGSASFEVKAHRTVTGTGSASGSNHD
                     VDKSFDVTADLTFHADQTATLVLDGMHTFTIDLKTGKITKVN"
     gene            1351..2733
                     /locus_tag="VF516_44750"
     CDS             1351..2733
                     /locus_tag="VF516_44750"
                     /inference="COORDINATES: protein motif:HMM:NF018269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HEX8114945.1"
                     /translation="MRKIRSRQWFHLAVCGGLLAGCAPGQGDPELGEAAAAVTAVGDA
                     LPGTDATGFAEAKAAFMTVEGLTDGLGPIFNERACGNCHTPGSIGGAGTQIERRFGTT
                     FFDFFFVEHFDPLANDGNGPFPARGGSLRQLQTVGSFTGLNGQACTVPLEHEPPEATI
                     HNVGRLTTPLFGLGLVDAMPDSFFDNLASAQPSSIRGIANRVKILLPNPTDPSQSLGG
                     TRVGRFGWKAGVATLTQFAADAYVNEMGITTQHCIKGTSVLDFATESQPNGIAQPAGC
                     DDLAPPQPAGNGIPAGTDDAVGSCAGGLTEIQDDVAEFAEFMTRLAPPPRGAITAAVT
                     AGQTVFQNAGCAGCHVLTTFTTPATPGNGVPGNFSFQPFSDFLVHDMGSLGDRIGNWG
                     DLIAVTRRMRTAPLWGLRFRTLFLHDGRTSDLATAITQHAGQGAAAAIAFNILSATNK
                     SNLIAFLQSL"
     gene            complement(2776..3246)
                     /locus_tag="VF516_44755"
     CDS             complement(2776..3246)
                     /locus_tag="VF516_44755"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0019439 - aromatic compound catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring-hydroxylating dioxygenase subunit
                     beta"
                     /protein_id="HEX8114946.1"
                     /translation="MLDPDYAELLQLQAAYAAAVDAGDWDAWAGLFIDDCRYRLQPRE
                     NFDRGLPLATLAFESKGMLHDRAYGIRETLFHDPYYQRHVVGPPLVRRREAGRIHTET
                     SYAVFRTKLSEPTIVFNVGRYLDEIVRTPDGLRFAVRLAIYDSELIPNSIIYPI"
     gene            3475..4287
                     /locus_tag="VF516_44760"
     CDS             3475..4287
                     /locus_tag="VF516_44760"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEX8114947.1"
                     /translation="MNTGEDCIAKAAEQFARDGAVVVRGALDAAWIQYLHAALDEALA
                     APTPLAQNLAGIQGGAFRSDMYLSSAFASFREFATRSPVGELGARMLGVGHVTLFNDE
                     LLVKEPGTPQETPWHHDMPYWPLGGDSVCSVWIPLDRVTRHVGALEFLRGSHRWGRRF
                     HPRNFDSGSDRPTDAREESLQAFIASDPDQVITTEADPGDCIVFHALTLHRAFGNTLT
                     DARRRAIVLRLVGPGVVYEPRPRTIPLIWAPSVAPGNPLGTDPELFPRLWPG"
     gene            complement(4513..5016)
                     /locus_tag="VF516_44765"
     CDS             complement(4513..5016)
                     /locus_tag="VF516_44765"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HEX8114948.1"
                     /translation="MSIRNALLIGILAAVVPSCALDGDGPDGPEAARAGDQPGTPDDQ
                     ASAADPETSIDSAAVTLASFPTCNSVRDWFNAAVPDWSGNGTVDCAMGEGADSPAVGR
                     LQRTMNVCYGERLTVDNDFGPATLAALKRTQTKAGTTADGVYGPNTRKAMLHQSNDNA
                     NTCIHVP"
     gene            complement(5200..6477)
                     /locus_tag="VF516_44770"
     CDS             complement(5200..6477)
                     /locus_tag="VF516_44770"
                     /inference="COORDINATES: protein motif:HMM:NF013044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEX8114949.1"
                     /translation="MTADPALPPAPEPLAEPEPRGLDRGRIPFATYTDEALYRRELDR
                     LFYRGHWCYVGLEAEVPEPGDFKRTQIGERSVLLTRDGDGALHVVENVCAHRGVAFCR
                     ERHGNRRTLVCPYHQWSYSLAGDLQGVPFRRGLRRAGEIQGGMPADFRLEDHGLTKLE
                     VATRGGAVFASFDHGVEPFEQFVGPAVLRYVDRLFSGRALRLLGYNRQRIPGNWKLMQ
                     ENIKDPYHPGLLHTWFVTFGLWRADNSAKLVMDRHHRHAAMISTRGNAGAASEVSQVA
                     SFKPQMTLADPRFLDIVHEPWWGDPTAVMLTLFPSVILQQQVNSVSTRQIVPVGPGVF
                     DFVWTHFAFADDDDAMVQRRLRQANLFGPAGFVSADDGEVIELSQRAFATSPGGHTTV
                     ELGGRDVGDADHMITETLIRGMYRYWQKVMWET"
     gene            complement(6509..7513)
                     /locus_tag="VF516_44775"
     CDS             complement(6509..7513)
                     /locus_tag="VF516_44775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114950.1"
                     /translation="MALCLLVFTVVRSKLQDAAPSQDPGSAGTGATVPPVPAVDEIEL
                     TRALRTNLEVVSWSPDEEAFVLDFSYARDASSPARWRVRAVIDAPSEKVLHWQDLSGA
                     GARVVSGEPRWPEPPAPSLPTEGFRPGSVLAPSRLHVRWCEKPSATRFLTGVRSIGQQ
                     VELSWDSSGAQVTPEACREGDAGSSLLYVDDSPWRLMRYSPRKGGTTAMTVYASPSHR
                     RAVVVTLDRHDDKLVTRFWTRLLGPQIHILSSNEELRRDGLRRLGVPGLLMSEDDKDP
                     VPPPSRILVPSDDDAAYPIAVALAEKLALTIERAPSDNRYMWGDVLIVLKPDEPPLRD
                     "
BASE COUNT         1203 a         2599 c         2706 g         1199 t
ORIGIN      
        1 gagcatcgcg cgcggccacc tgccgctcgg cttcctgcat gacagcgatg gccacttccg
       61 cggcaccctg atgggcgtcg gccacgactt caccgtgacg tgcaaggacg ccgccggcac
      121 cgtgcagacc gtgtgcaaca gcatgaccga tagcgcgacg gtcaccgtca aggtgaccgg
      181 caacctgcag accacgaact tctcgtcgat gatcgaccgc gaaggcaact tcacgatcac
      241 cggcctgcag ggcgcgaccg cgacgttcaa cggcgacagc agcttctcgc tcgacacctc
      301 gctgacgtcg gtgttccacc cgggcgtgac gtcgaccttc acgttcgacg cgaccgcctc
      361 gtacaaggcg atcacgatcg cgaccgcgga tcacacgatc accggcggct cggcgagctt
      421 cgaggtcaag gcccatcgca cggtgaccgg caccggctcg gcctcgggct cgaaccacga
      481 cgtcgacaag tcgttcgacg tgacggccga cctgacgttc catgccgacc agaccgcgac
      541 gctggtgctc gacggcatgc acacgttcac gatcgacctg aagaccggca agatcaccaa
      601 ggtcaactga tcgcgcgacg gctgcccggg gcgtaccagc gcgacgtgcc accgccgcgc
      661 gcaccagctc gatccgggat ccctcgagcc gccgcagcta gacggtccgc gcggaccaga
      721 ggccacgaca gcgttggctg tatgccgcgc gccggatccg ctcggtgctc gctggctggg
      781 cgagctcggg tgtgcgcgag cgctcgccga actgcccgtg gcgcgagcgt aaggagcatc
      841 acacgcaagg tctgcggtgg attgccactc tcgattggca ctctgcgctc gctgccctca
      901 gataactgac gcgactcgat agcaagtgac aaaaaaccgt gggggcgcgc agatcgagga
      961 accgaccggt tccgcatcgt ggcgcccgtc gagcaaatat cgacgctgac ctcgagcacc
     1021 cgaccggtat ttcgagctgg cacgtcgtgc ggcgccgcga tgtgctgcaa gattgctttg
     1081 gcgggtcatg gggttttgcg gagttgaaat cgattttcaa taaaacatga ttaaggattg
     1141 actctgatca gagtcacgcc tagggtcact ttgcccgctg tgctctggcc gcgacgcctg
     1201 ggccccagcg agtgacgagc agggtcctcc gcagcggttg cggatcgggc catcggttgt
     1261 ccacgcgacg gcgttcgccg cggacggggc tcgtctgccc gggcatggac gcgatgcgtc
     1321 atgtcacccg attaccgtag ggaggtagca atgcggaaga ttcgatctcg acagtggttc
     1381 cacctcgccg tgtgtggggg gctgctggcc ggctgtgcgc ccggccaagg cgatcctgag
     1441 ctgggagagg ccgccgccgc ggtcaccgcg gtcggcgatg cgctgccggg gaccgacgcc
     1501 accggattcg ccgaggcgaa ggcggcgttc atgacggtcg aagggctgac cgacggactg
     1561 ggcccgatct tcaacgagcg cgcgtgtggc aactgccata ccccgggctc gatcggcggc
     1621 gccggcacgc agatcgagcg ccggttcggc acgaccttct tcgacttctt cttcgtggag
     1681 cacttcgatc cgctcgccaa cgatggcaac gggccgttcc ccgcccgcgg cggatctctc
     1741 cgccagctcc agaccgtcgg cagcttcacc ggtctcaacg gtcaagcctg cacggtgccg
     1801 ctcgagcacg agccacccga ggcgacgatc cacaacgtcg gccgcctgac cacgccgctg
     1861 ttcggcctcg gcctggtcga cgccatgccc gactcgttct tcgacaacct cgcgtcggcg
     1921 cagccgtcga gcatccgcgg catcgcgaac cgggtgaaga tcttgctgcc gaacccgacc
     1981 gacccgtcgc agagcctggg tggcacccgc gtcgggcggt tcggctggaa ggccggcgtc
     2041 gcgacgctga cccagttcgc ggccgacgcc tacgtcaacg agatgggcat caccacccag
     2101 cactgcatca agggcacgtc ggtgctcgac ttcgcgaccg agtcgcagcc caacggcatc
     2161 gcgcagccgg cggggtgtga cgacctcgcg ccgccgcagc cggccggcaa cggcatcccg
     2221 gccgggacgg acgacgcggt tggcagctgc gccggcggcc tgaccgagat ccaggatgac
     2281 gtcgccgagt tcgccgagtt catgacgcgc ctggcgccgc cgccgcgcgg cgcgatcacc
     2341 gccgccgtca ccgccggcca gaccgtgttc cagaatgccg gctgcgcggg ctgccacgtg
     2401 ctgaccacgt tcacgacgcc ggcgaccccc ggcaacggcg tcccgggcaa cttctcgttc
     2461 cagccgttct cggacttcct ggtccacgac atgggctcgc tcggcgaccg gatcggcaac
     2521 tggggcgatc tgatcgcggt gacccgccgg atgcgcacgg cgccgctgtg gggcctgcgg
     2581 ttccgcacgc tgttcctgca cgacggccgg accagcgacc tcgcgaccgc gatcacgcag
     2641 cacgccggtc agggcgccgc cgccgccatc gcgttcaaca tcctgagcgc gacgaacaag
     2701 agcaacctga tcgcgttcct gcagtcgctg tgaccggcgg cgagcccgcc ggacaccgcg
     2761 gccgtcggat cgacttcaga tcggatagat gatcgagttc gggatcagct cgctgtcgta
     2821 gatcgcgagc cggacggcga agcgcaggcc gtccggcgtg cggacgatct cgtcgaggta
     2881 gcgcccgacg ttgaacacga tcgtcggctc cgacagcttg gtgcggaaca ccgcgtagga
     2941 cgtctcggtg tggatccggc cggcctcgcg ccggcggacc agcggcgggc cgacgacgtg
     3001 gcgctggtag tacggatcgt ggaacagggt ctcgcggatg ccgtaggcgc ggtcgtggag
     3061 catgcccttg ctctcgaacg ccagggtggc gagcggcagg ccgcgatcga agttctcgcg
     3121 cggctggagc cggtagcggc agtcatcgat gaacagcccg gcccatgcgt cccagtcgcc
     3181 ggcgtcgacc gcagcggcgt aggcggcctg gagctggagc agctcggcgt agtcgggatc
     3241 gagcacggcg agagcttgtc cgatcgtcca ggggccatca accgcccgtc cgccctgaag
     3301 cagcgcccac gtcggtcaac agcatcgcaa gcaaccgtgg gattcgctgc gcggcaggtc
     3361 gggtgtagaa atgcgcctgg acttcactgc tcgggaggca ggcagaaccg gcgaggcgtt
     3421 cggttcctgg cggcgaacca cgcttccgga tcgcggcgac gtacaatgca cgtcatgaac
     3481 accggcgagg attgcatcgc caaggccgca gagcagttcg cccgcgacgg agccgtggtc
     3541 gtgcgcggtg ctctggacgc agcgtggatc cagtatctcc acgctgcgct cgacgaggcg
     3601 ctcgccgctc cgacgccgct ggcccagaat ctcgccggaa tccagggcgg cgcgttccgc
     3661 agcgacatgt acctgtcgtc ggcgtttgcg agctttcgcg agttcgcgac gcggtcacca
     3721 gtcggcgagc tcggcgcgcg catgctcggc gtcggacacg tgaccttgtt caacgacgag
     3781 ttgctcgtga aggagccggg cacgccgcag gagacgccgt ggcatcacga tatgccgtac
     3841 tggccgctcg gcggcgacag cgtctgctcg gtctggatcc cgctcgatcg cgtgacccgg
     3901 cacgtcggtg cgctcgagtt ccttcgcgga tcgcaccggt ggggccgccg gtttcatccg
     3961 cgcaactttg attcgggatc cgatcgaccg accgacgcgc gcgaggagag cctgcaagcg
     4021 ttcatcgcga gcgatccgga tcaggtcatc accaccgagg ccgatccggg cgactgcatc
     4081 gtgttccacg cgctcacgct ccaccgggcg ttcggcaaca ccctgacgga cgcgcgtcgc
     4141 cgggcgatcg tgcttcgact cgtcggccca ggcgtggtct atgagccgcg tccccgcacg
     4201 atcccgctga tctgggcgcc ttcggtcgca ccgggaaatc cgctgggcac cgatcccgag
     4261 ctgttccctc gactgtggcc gggctaggtg gcctggatca tcttctgcca gtagcggtac
     4321 atcccgcgga tcagggtctc ggtgatcatg tgatcggcgt cgccgatgtc gcggccgctg
     4381 ctcagcgtag tcggggtcga tcacgccatg accttcggcc agcggagggt acctcgagac
     4441 catgcatcca cgaatccggt gtggttgcat cgggcggggt agcactctgg cctgggaccc
     4501 gcggatcgat gactacggca catgaatgca ggtgttggcg ttgtcgttgc tctggtggag
     4561 catggccttt cgcgtattgg gaccatatac gccgtctgcc gtggtgccag ccttggtctg
     4621 cgttcgcttg agcgccgcca gggtggcggg gccgaagtca ttatcgacgg tcaagcgctc
     4681 gccgtaacac acattcatcg tcctctggag tcgacccacg gcggggctat ctgcgccttc
     4741 ccccatggcg caatccacgg tgccgttgcc gctccagtcg ggcacggccg cattgaacca
     4801 gtcccggacg gagttgcagg tcgggaacga cgcgagcgtg acagccgccg aatcgatcga
     4861 cgtctccggg tccgcggcgg aggcctggtc atccggagtg ccgggctgat cgccggctct
     4921 ggctgcctcg gggccgtccg gcccgtcgcc atcgagcgcg caggacggaa cgaccgctgc
     4981 gagaatacca atcagaagtg cgttgcggat ggacatcgct ttcatatcaa cggctcctcg
     5041 gggccatgtc atggcggccc tcatggggat cgaccggggg tcccgtgata tgtcgcggca
     5101 cgaatgagca gaacacggtc ggcgccgcag tctgccgcaa cgcggtgcgt tacaacctcg
     5161 cagcgacgtg ggatccgggc cgggccgttg gaggccgagc tatgtctccc acatgacctt
     5221 ctgccagtag cggtacatgc cgcggatcag ggtctcggtg atcatgtggt cggcgtcgcc
     5281 gacgtcgcgg ccgccgagct cgacggtggt gtggccgccg gggctggtgg caaacgcgcg
     5341 ctgcgacagc tcgatgacct cgccgtcatc ggccgacacg aagccggccg ggccgaacag
     5401 gttggcctgg cgcagccggc gctggaccat cgcgtcatcg tcgtcggcga acgcgaagtg
     5461 cgtccacacg aagtcgaaca cgccggggcc gaccggcacg atctggcggg tcgacacgct
     5521 gttgacctgc tgctgcagga tcacgctggg gaacagggtg agcatcaccg cggtcggatc
     5581 gccccaccac ggctcgtgga cgatgtcgag gaaccgcggg tcggcgagcg tcatctgcgg
     5641 cttgaagctg gcgacctgcg agacctcgga cgccgcgccg gcgttgccgc gggtcgagat
     5701 catcgcggcg tggcggtggt ggcggtccat gaccagcttc gcgctgttgt cggcgcgcca
     5761 cagcccgaag gtgacgaacc aggtgtgcag gaggcccggg tggtacgggt ccttgatgtt
     5821 ctcctgcatc agcttccagt tgccggggat gcgctggcgg ttgtagccga gcaggcgcag
     5881 cgcgcggccg ctgaacagcc ggtcgacgta gcgcagcacc gccgggccga cgaactgctc
     5941 gaacggctcg acgccgtgat cgaacgacgc gaacaccgcg ccgccgcggg tcgcgacctc
     6001 gagcttggtc aggccgtggt cctcgaggcg gaagtccgcc ggcatgccgc cctggatctc
     6061 gccggcgcgg cgcaggccgc ggcgaaacgg cacgccctgg aggtcgccgg ccaggctgta
     6121 gctccactgg tgatacgggc agaccagcgt cctgcggttg ccgtggcgct cgcggcagaa
     6181 cgcgacgccg cggtgggcgc agacgttctc gacgacgtgc agcgcgccgt cgccgtcgcg
     6241 ggtcagcagg accgagcgct cgccgatctg ggtgcgcttg aagtcgccgg gctcggggac
     6301 ctcggcctcc aggccgacgt agcaccagtg gccgcgatag aacagccgat cgagctcgcg
     6361 gcggtacagc gcctcgtcgg tgtaggtcgc gaacgggatc cggccgcgat cgaggccgcg
     6421 gggctccggc tcggcgagcg gctcgggggc cggcggcagg gcggggtcgg cggtcatggc
     6481 gcgatgatcg agcgccgccg gcgaaccgtc aatcgcggag tggcggctcg tccggcttga
     6541 gcacgatcag gacatcgccc cacatgtacc ggttatcgga cggtgcacgc tcgatcgtga
     6601 gcgcgagctt ctcggccaga gcgaccgcga tcggataggc agcgtcatcg tcggacggca
     6661 ccaggatcct cgagggcgga ggcaccggat ccttgtcgtc ctcggacatc agcaagcccg
     6721 gcacgcccag tcgccgcagc ccgtcgcggc gcagctcctc gttgctggac aggatgtgga
     6781 tctgggggcc gagcaggcgg gtccagaacc gcgtgacgag cttgtcgtcg tgacgatcga
     6841 gcgtgaccac cacggcgcgg cgatggctcg gggatgcgta gaccgtcatc gcggtcgtgc
     6901 cgcccttcct cgggctgtag cgcatcagtc gccacggcga atcgtcgacg tacaggagcg
     6961 agctgccggc gtcgccttcg cgacatgcct cgggcgtgac ctgcgcgccg ctggagtccc
     7021 agctgagctc gacctgctgc ccgatcgatc gaacgccggt gaggaaccgg gtggccgatg
     7081 gcttctcgca ccatcgcacg tggagccgcg agggtgcgag cacgctgccg ggccggaagc
     7141 cttccgtcgg gagcgagggc gctggaggct ccggccatct cggctccccc gagacgacgc
     7201 gcgcgcctgc gcccgagagg tcctgccagt ggagcacctt ctcgctgggg gcatcgatca
     7261 cggcgcggac gcgccatcgc gcgggactcg aggcatcgcg ggcatagctg aagtcgagga
     7321 caaaggcctc ttcatccggg ctccagctga cgacctcgag gttcgtccgc agggcccggg
     7381 tcagctcgat ctcgtcgacg gccggcaccg ggggaacggt cgccccggtg cccgccgagc
     7441 ccgggtcctg ggacggggcc gcgtcctgga gcttcgatct caccaccgtg aacaccagga
     7501 ggcacagagc catcagggcg atcgcgacga tgtacctgcg atggacaccg gggcctccga
     7561 gggcaccgct cccacccggc gtcaccggag ccggcgtgcg caccgactgt gggctggggg
     7621 tcgatggcgg cggcggcggt tgcggctgtg cgcgggccgg aggcggcggc ggttgggtct
     7681 gcgcgggagc gggaggcgcc ggcggct
//
LOCUS       DASVOR010002440         3840 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_494736,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002440 DASVOR010000000
VERSION     DASVOR010002440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494736"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..524
                     /locus_tag="VF516_44780"
     CDS             <1..524
                     /locus_tag="VF516_44780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEX8114951.1"
                     /translation="NSSDVPLVFLQNITGFMVGKKYEQEGIIKHGAKLINAVSNSTVP
                     AITIMTGASYGAGNYAMCGRAYAPRFLFTWPNHRIAVMGGKQLAGVLDIIQREAAAKR
                     GEPVDEQKLGVMKAMTESMIEKESDPYFATARLWDDGIIDPRDTRTVLAIALSAAYTA
                     PVRGTTSWGVFRH"
     gene            complement(565..2346)
                     /locus_tag="VF516_44785"
     CDS             complement(565..2346)
                     /locus_tag="VF516_44785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114952.1"
                     /translation="MSIRAFTISMRCLVLLLIVGCGDDLHARPDAGSIPDPTADSGAP
                     LPDAPPTEMATPLERLNWRNARGAPIATSTIAIPAAEMFAVSGDGFVTRSGCQPPQIC
                     SLTWRDLAGTPGIQRDHMTRVTVTSVSPDGKSALLVALDAPGTLEPCFTAQSAFLVAR
                     GALQLLDLATGAASSELPLRSNLWSAPGFTPFSDWFFAAPIEGTACVASTTGVRSTTS
                     PFAPPPGLDANDQFVQIVDAHRWLVIRNGTNLGLSDPLTPGSFSFLGEDPSRFDVTQG
                     WVHVYLGFGNLAQDVVSIPPAGPMRQTTLRDEDWFPFGSRGRWVRVCGFLQPDGERPC
                     RVVDAQAEVAPVDYRASFSSDHPNDDAVLLDSGAVVFVGPTEDGTLAVQRTVPATGHR
                     EILHHGNGTLRSLGDGAAALLLQDEQAWLIEAHREELVATRVSQVVSMPQLPPLGRVP
                     GRQDDLAFLVSLLDDGRFTLAILDVRTRRLATVTDHLYFTPPIGPPFAFDDGCGQPWT
                     TRNSGSVVEGLFQQPQHLFFVETGTPAKLWLLPIDLSAPPRRLADLAGDPVSCHAPLA
                     SPDGRRLGFAENGADGTTTRITLSSQD"
     gene            complement(2459..3211)
                     /locus_tag="VF516_44790"
     CDS             complement(2459..3211)
                     /locus_tag="VF516_44790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114953.1"
                     /translation="MKKASSTPKRLVIGGIVVGLVVVALFMFMREARAPERTAGEQTV
                     ARTTEVQVPMPGPNPSLPPKSFELPVAMPDQIIEQILKEDEKLGLFMDYRKMVLPNEK
                     TRDEYRKLLSDPEMMTAMAEDLMDPGSGHPESKEYYRRLMQIDYFEAALAWKDNPGRQ
                     KVLQLTRDIITKDNFQGDQDSSRRQMLGGSKMELYRLLYEQDAQKAKELVAQAKGTRM
                     EPLVSWMSEEELRRRTREEEIRKEVRDSAGKN"
     gene            complement(3312..>3840)
                     /locus_tag="VF516_44795"
     CDS             complement(3312..>3840)
                     /locus_tag="VF516_44795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEX8114954.1"
                     /translation="CYTPDQSLRFSTGDVNILDAFNALRDDVVTLSSDATLNNPKKQV
                     SRVYSYTREIRDVEQPIFKVTTQSGGSLSVTNEHPILVSNGHDGRLVKAEKIHEGDRL
                     VKADGTPDPVVHIEKTTFFGKVYNIKPAPEELVSNILIAQGFLVGSARFQNDDIMYMN
                     RSLLFRSVPDDVMPK"
BASE COUNT          655 a         1206 c         1327 g          652 t
ORIGIN      
        1 gcaactcgag cgacgtgccg ctggtgttcc tccagaacat caccggcttc atggtcggca
       61 agaagtacga gcaggagggc atcatcaagc acggcgccaa gctgatcaac gcggtgtcga
      121 actcgaccgt ccccgcgatc acgatcatga ccggcgcgag ctacggcgcc ggcaactacg
      181 cgatgtgcgg ccgggcctac gcgccccggt tcctgttcac ctggcccaac caccggatcg
      241 ccgtgatggg cggcaagcag ctcgccggcg tgctcgacat catccagcgc gaggccgcgg
      301 ccaagcgcgg cgagccggtc gacgagcaga agctcggcgt catgaaggcg atgaccgaga
      361 gcatgatcga gaaggagagc gatccgtact tcgcgaccgc gcggctgtgg gacgacggca
      421 tcatcgaccc gcgcgacacc cgcacggtgc tcgcgatcgc gctgtcggcg gcctacaccg
      481 cgccggtccg tggcacgacg tcgtggggcg tgttccggca ctgagctccc gaccgctgtg
      541 caccgctgca gcggacaggc gcgctcagtc ctgcgatgac agcgtgatcc gcgtggttgt
      601 cccgtcagcg ccattctcgg cgaagccgag gcggcgtccg tcgggactgg cgagcggtgc
      661 atgacacgag acgggatcac cggcgagatc ggcgagacgg cgaggcggcg ccgagaggtc
      721 gatcgggagc agccacagct tcgccggcgt gcctgtttcg acgaagaaca ggtgttgcgg
      781 ctgctggaac aggccctcga ccacgctgcc gctattgcgc gtggtccacg gctgcccgca
      841 gccgtcgtcg aaagcgaacg ggggcccgat cggtggcgtg aagtacaggt ggtcggtcac
      901 ggtggccagg cgtcgcgtgc gcacgtcgag gatcgcgagc gtgaaccgac catcatccaa
      961 caacgagacc aggaacgcca gatcgtcctg ccgtccgggg acgcgcccca gcggtggtag
     1021 ctgcggcatg ctcaccactt gacttacacg cgtggccacg agctcctcgc ggtgggcctc
     1081 gatgagccat gcctgctcgt cctggagcag cagcgccgcc gcgccatcgc ccagcgaccg
     1141 cagcgtgccg ttgccgtgat gcaggatctc gcggtgtccg gtggcgggca ccgtgcgctg
     1201 cactgcgagc gtgccgtcct cggtggggcc gacgaagacg acggcgccgc tgtcgagcag
     1261 gaccgcgtcg tcattgggat ggtcggacga aaagctggcg cgatagtcca cgggcgcgac
     1321 ctcggcctgg gcatcgacca cgcggcaggg gcgttcgccg tcaggctgga ggaacccgca
     1381 gacccggacc cagcgccccc gcgagccgaa gggaaaccag tcttcatcgc gcagcgtggt
     1441 ctgtcgcatc ggcccggccg ggggaatcga aaccacgtcc tgggccagat tgccaaatcc
     1501 gagatacaca tgcacccacc cctgggtgac gtcgaaccgg gacggatctt cgccgagaaa
     1561 gctgaagctg cccggcgtca gcggatcgga gagcccgagg ttcgtgccgt tgcgaatgac
     1621 gagccaacgg tgcgcgtcga cgatctggac gaactggtca ttcgcgtcga gccccggcgg
     1681 cggcgcgaat ggcgaggtcg tggaccgaac gcccgtcgta ctggcgacgc acgccgtccc
     1741 ctcgatgggc gcggcgaaga accagtcgct gaacggcgtg aatcccggcg cggaccagag
     1801 gttgctgcga agcgggagct cggagctggc cgcgccggtg gcgagatcga gcagctgcag
     1861 cgctccgcgc gccaccagga atgcgctctg ggcggtgaag cagggttcga gcgtgccggg
     1921 cgcgtcgagc gcgacgagga gcgcgctctt gccgtccggc gatacggagg tgacggtcac
     1981 gcgggtcatg tgatcgcgct ggatgcccgg ggtgcccgcg aggtcgcgcc aggtaaggct
     2041 gcagatctgg gggggctggc accccgaccg cgtcacgaag ccgtccccgg agaccgcgaa
     2101 catctcggcg gccgggatgg cgatggtgct ggtggcgatg ggcgcaccgc gcgcgtttcg
     2161 ccagttcagg cgctccaggg gagtcgccat ctccgtcggt ggcgcgtcgg ggagcggcgc
     2221 gccggagtcc gcggtcgggt cgggtatgga gcccgcgtcg ggccgcgcat gtaggtcgtc
     2281 gccgcacccg acgatgagaa ggaggacgag gcaccgcatg gagatcgtga aggcgcgtat
     2341 ggacatgggc cctgttgttc gcgaagtgca ccaaggacag atccgtctgg ggcacctcgt
     2401 ctattatctc agatgacggg cgaggaggaa aggccagtcg ccgcaaccga gagggaggtc
     2461 agttcttccc cgccgagtcc ctcacttctt tcctgatctc ttcctctcgg gtgcgccgcc
     2521 ggagctcctc ttcgctcatc cagctgacca gtggctccat gcgggtaccc ttggcctggg
     2581 ccaccagctc ctttgctttc tgtgcgtcct gctcgtacag caagcgatag agctccatct
     2641 tggaccctcc gagcatctgt cggcgggagc tatcctggtc accctggaag ttgtccttgg
     2701 taatgatgtc tcttgtcagc tggagcacct tctggcgccc cggattgtcc ttccaggcga
     2761 gcgccgcctc gaagtagtcg atctgcatca gccggcggta atactccttc gactcggggt
     2821 gaccgctgcc cgggtccatc aagtcctcgg ccatggcagt catcatctcg ggatccgaca
     2881 atagcttgcg gtactcgtca cgcgtcttct cattgggcag gaccatcttg cggtagtcca
     2941 tgaacaaccc gagcttctcg tcctccttga ggatctgctc gatgatctgg tccggcatcg
     3001 ccacgggcag ctcgaaggat ttgggtggca acgaagggtt cggcccaggc attgggacct
     3061 ggacctcggt cgtgcgggcg acggtctgtt cacccgcggt gcgctctgga gctctggcct
     3121 ctctcatgaa catgaacagt gcgacgacga caagacccac cacgatacca cctatcacga
     3181 gtcgcttggg ggtcgacgat gcttttttca tgaaaacggc tctcctcgaa aagaaggact
     3241 gcagggcacc cggagggtcc ggcgcccatc caggacggac gccggacctt tggaggacaa
     3301 ctatctacgg gttacttggg catcacgtcg tcgggcacag agcggaacag cagggagcgg
     3361 ttcatgtaca tgatatcatc gttctggaac cgggccgagc cgaccaggaa accttgcgcg
     3421 atgaggatgt tggagacgag ctcttccgga gcgggcttga tattgtagac cttgccgaag
     3481 aaggtcgtct tctcgatgtg gacgaccgga tccggcgttc cgtcggcctt gacaagacga
     3541 tcgccctcgt ggatcttctc ggccttgacc aggcggccgt cgtggccatt gctgacgagg
     3601 atggggtgct cgttggtgac gctcagcgag ccgcccgact gggtggtcac cttgaagatc
     3661 ggctgctcga cgtcgcggat ctcgcgcgtg tagctgtaga cgcgggagac ctgcttcttg
     3721 ggattgttga gggtcgcgtc agacgacagc gtgacgacat cgtcccgcag cgcgttgaag
     3781 gcatcgagga tgttcacgtc gccggtgctg aaccgcaggc tctggtcggg cgtgtagcag
//
LOCUS       DASVOR010002441         1617 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_143674,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002441 DASVOR010000000
VERSION     DASVOR010002441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143674"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..1517
                     /locus_tag="VF516_44800"
     CDS             168..1517
                     /locus_tag="VF516_44800"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEX8114955.1"
                     /translation="MTTSWRLEAEAEISRGIAARRFRLGNGLGLITALDRRAPIVSLQ
                     TWYRVGSRHERPGATGMAHLFEHLMFGPTDSLPPGEFDRLVERTGGESNAATWVDWTY
                     YRLSLPARDLPLGIRLESGRMRHLVLEPTPVEAERDVVTNERRERVEDDVDGWLDEQL
                     MAHAFTEHPYRWPTIGWMEDIRALSLPDIRAFYRTWYAPNNATLVCVGEFDEAAVLDL
                     VERSYGAIAPATMPAVAAVREPEQTRERVVRAPKPIATDRLLVGYKAPGQDDSDWAVL
                     EIVATLLAGCPSARLYRRLVIDREAASSVDAQLTPFRDPSLLRLAVTASRGHTADEIL
                     AAIDRELAAMAEQPPRHAEVEKAKAIAETDFWTSLVDIDGKAEALGHYETALGDFRKV
                     NAIAERLAAVTADDVARCVRAYLRADRRTVVIAEPESPDLDGGEPGDPGDEQDDDAE"
BASE COUNT          220 a          606 c          583 g          208 t
ORIGIN      
        1 cccggaccgg tcgggatgcg cgcgcccggc gagctgttcc gcgccgagcc cgcgctgcgc
       61 gcgatcgcgc acgctgcgga cttgacgatc gagccgagtt ttggttagca gcgcacgccg
      121 cgcgcgacgg ccgcccggcg ctcccggcgc cgtggtatcc atcaccgatg acgacgtcgt
      181 ggcgcctcga ggccgaggca gagatcagcc gcgggatcgc ggcgcggcgg ttccgcctgg
      241 gcaatggact gggcctgatc acggcgctcg accgccgcgc gccgatcgtg tcgctgcaga
      301 cctggtaccg cgtcggctcg cgccacgagc gccccggcgc gaccggcatg gctcacctgt
      361 tcgagcacct gatgttcggg ccgaccgaca gcctgccgcc cggcgagttc gaccggctgg
      421 tcgagcgcac cggcggcgag tccaacgcgg cgacctgggt cgactggacc tactaccggc
      481 tgtcgctgcc ggcgcgcgac ctgccgctcg ggatccggct cgagtccggc cggatgcggc
      541 acctggtgct ggagccgacc ccggtcgagg ccgagcgcga cgtggtgacc aacgagcgcc
      601 gcgagcgggt cgaggacgac gtcgacggct ggctcgacga gcagctgatg gcccacgcgt
      661 tcaccgagca cccttaccgc tggccgacga tcggctggat ggaggacatc cgcgcgctgt
      721 cgctgcccga catccgcgcg ttctatcgca cctggtacgc gcccaacaac gcgaccctgg
      781 tgtgcgtcgg cgagttcgac gaggccgcgg tgctcgatct ggtcgagcgc agctacggcg
      841 cgatcgcccc ggcgacgatg ccggcggtcg cggcggtccg cgagcccgag cagacccgcg
      901 agcgcgtggt ccgcgcgccc aagccgatcg cgaccgatcg cctgctggtc ggctacaagg
      961 cgcccggcca ggacgactcc gactgggcgg tgctcgagat cgtcgcgacc ctgctcgcgg
     1021 gctgcccgtc ggcgcggctg taccgccggc tggtgatcga ccgcgaggcc gcgtcgtcgg
     1081 tcgacgccca gctcacgccg ttccgcgacc cgtcgctgct ccggctggcg gtcaccgcgt
     1141 cgcgcggcca caccgccgac gagatcctcg cggcgatcga ccgcgagctc gccgcgatgg
     1201 ccgagcagcc gccgcgccac gccgaggtcg agaaggccaa ggcgatcgcc gagaccgact
     1261 tctggacctc gctggtcgac atcgacggca aggccgaggc gctcggccac tacgagaccg
     1321 cgctcggcga cttccgcaag gtcaacgcga tcgccgagcg gctcgccgcg gtcaccgccg
     1381 acgacgtcgc gcgctgcgtg cgggcctacc tgcgcgccga ccgccgcacc gtcgtgatcg
     1441 ccgagcccga gtcgcccgat ctcgacggcg gagagcccgg cgatcccggc gacgagcagg
     1501 acgatgatgc agaatgaccc gatgatcatc gtcgagccgt cgcccgacac gccgctggtg
     1561 tggttcgaga tcgcgatccg cggcggcgcc gcgctcgatc cgccgggcat cgagggc
//
LOCUS       DASVOR010002442         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_85578,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002442 DASVOR010000000
VERSION     DASVOR010002442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85578"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /locus_tag="VF516_44805"
     CDS             complement(<1..1379)
                     /locus_tag="VF516_44805"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEX8114956.1"
                     /translation="MRRASWVSLVSLAAFAIVLLPVQASADRPGFDPASIYKVPLGDA
                     PVSGPADAPVTIVAWSDYACQYCNYVQATLDHLARLYPGQLRWVHRTLPLDEDNTLTA
                     EAALAAGAQGRFRPMNDRLYALAGHVDRPAVELIARELGLDMVRFRADLDSRAYRASI
                     TADQAAARALGITGTPTFFINGRPLHGNRPLTEFVDVVEQELARAAEQPAGPGRYGAL
                     VAGGKPTADAPYDAKGPRRTLEGTTIYRVGLGLPGHQVGPDDALVTIVVWSDFQCPFC
                     ARMAPVLAHLRAQYGDDVRIIYRHAAMSFHRNAALAAEAAVAAAEQGKFWAFHDQVFG
                     DFGHLERADLERFAAAAGLDMARFRAALDDHRYRDAVIAETAAGEALGVDGTPTTFLN
                     GMPVGGARDAQPMERLVEAHLGRSAGMLKAGVTRADLYAVLMTLATGDDLADPSRIPS
                     IVDHQIELRG"
     gene            complement(1379..>1851)
                     /gene="tsaE"
                     /locus_tag="VF516_44810"
     CDS             complement(1379..>1851)
                     /gene="tsaE"
                     /locus_tag="VF516_44810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEX8114957.1"
                     /translation="LGRPVRVKLADPAATRRVGERLAGLVRGGDTIALVGELGAGKTT
                     LVAGLVAGLGGGAAASPTFSLVNEYPGGRLVVWHVDLYRIERAAELPELGLDDVIGDP
                     RGICVVEWADRFAVLPPDHLRIELAHTPDGGRGLAASGTGPRGRELAAELVGSG"
BASE COUNT          240 a          670 c          716 g          225 t
ORIGIN      
        1 ccgcgcagct cgatctggtg atcgacgatc gacgggatcc gcgacggatc ggcgaggtcg
       61 tcgccggtcg ccagggtcat cagcacggcg tagaggtcgg cccgggtcac gccggccttc
      121 agcatgccgg ccgagcgccc caggtgggcc tcgaccaggc gctccatggg ctgggcatcg
      181 cgcgcgccgc cgaccggcat gccgttgaga aacgtcgtcg gcgtgccgtc gacgccgagc
      241 gcctcgcccg ccgcggtctc ggcgatcacc gcgtcgcggt agcggtggtc gtcgagcgcg
      301 gcgcggaacc tcgccatgtc gagcccggcc gcggccgcga accgctccag gtcggcgcgc
      361 tccaggtggc cgaagtcgcc gaacacctgg tcgtgaaacg cccagaactt gccctgctcg
      421 gcggccgcga ccgcggcctc ggcggcgagc gcggcgttgc ggtgaaagct catcgcggcg
      481 tggcggtaga tgatgcgcac gtcgtcgccg tactgggcgc gcaggtgggc gagcaccggc
      541 gccatccggg cgcagaacgg gcactggaag tcgctccaca ccacgatcgt gaccagcgcg
      601 tcgtcggggc cgacctggtg gccgggcagg ccgaggccga cgcggtagat cgtggtgccc
      661 tcgagcgtgc ggcgcgggcc cttggcgtcg tacggcgcgt cggcggtcgg cttgcccccg
      721 gcgaccagcg cgccgtagcg ccccgggccc gccggctgct cggccgcgcg cgcgagctcc
      781 tgctcgacga cgtcgacgaa ctcggtcagc ggccggttgc cgtgcagcgg gcggccgttg
      841 atgaagaacg tcggcgtgcc ggtgatgccg agcgcgcggg cggcggcctg atcggcggtg
      901 atcgacgcac ggtacgcgcg gctgtcgagg tcggcgcgga accgcaccat gtcgaggccg
      961 agctcgcggg cgatcagctc gacggccggg cggtcgacgt ggccggccag cgcgtacagc
     1021 cggtcgttca tcggccggaa ccggccctgc gcgccggcgg cgagcgcggc ctcggcggtg
     1081 agcgtgttgt cctcgtcgag cggcagcgtg cggtgcaccc agcgcagctg gccgggatag
     1141 agccgcgcga ggtggtcgag cgtggcctgg acgtagttgc agtactggca ggcgtagtcg
     1201 ctccacgcga cgatcgtgac cggggcgtcg gccgggccgc tgaccggcgc gtcgccgagc
     1261 ggcaccttgt agatcgacgc ggggtcgaag ccggggcggt cggccgaggc ctggaccggc
     1321 agcaagacga tcgcgaacgc ggcgagcgag accagcgaga cccatgacgc gcggcgcatc
     1381 accccgagcc taccagctct gccgccagct cgcgaccgcg cgggccggtg ccgctcgccg
     1441 cgaggccgcg gccgccgtcc ggcgtgtggg cgagctcgat ccgcaggtga tcgggcggca
     1501 gcacggcgaa ccggtcggcc cactcgacga cgcagatccc gcgcgggtca ccgatcacgt
     1561 cgtcgagccc gagctcgggc aactcggcgg cccgctcgat ccggtacagg tcgacgtgcc
     1621 acaccacgag ccggccgccg gggtactcgt tgaccagcga gaacgtcggg ctcgccgccg
     1681 cgccgccgcc gagcccggcg accaggccgg cgaccagcgt ggtcttgccg gcgccgagct
     1741 cgccgaccag cgcgatcgtg tcaccgccgc gcaccagccc ggcgagccgc tcgccgacgc
     1801 gccgggtcgc tgccggatct gcgagcttca cgcgaaccgg tcgtcccagt g
//
LOCUS       DASVOR010002443         3436 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_494752,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002443 DASVOR010000000
VERSION     DASVOR010002443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494752"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3144
                     /locus_tag="VF516_44815"
     CDS             <1..3144
                     /locus_tag="VF516_44815"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8114958.1"
                     /translation="PALRPSDGANLAAALTALGPRVRSATVAPHRNPVRRSALTGGER
                     RLLSVVRLATSDRDTLAADELQRAIRPYGGHMEQLADGSTIVVIEPDQQTDYMVATDQ
                     AAQAARCALAIRALIADRPMAIAMGRVESTGQRPGGEVIDRASRLLADSRQAAAVSMP
                     IALDEISAGLLDARFDVVEVGSGLMLRGERALIHGARTLLGRPTACVGRDWELNALMS
                     ILDECIDEREARAVIVTAAAGMGKSRLAAELVTRIQQRGEPVAVWVARGDSLRAGSTL
                     GLLAQALRGVLGLPGGESLAVRHDKIRTWVAEHAVESERKRVAEFLGELIGAPFPADG
                     AAGASLRAARHDAQLMSEQMRRAWMDFLDAEAAAHPVLLVLEDLHWGDFGTVRFIDTA
                     LRDRRERPWMVLALARPEVFQMFPKLWAERHNVQEIRLKELGRKAGERLVCQVLGDRV
                     GPDTIKRLVRQAEGNAFYLEELIRAAAEGKDGALPDTVLAMVETRLGRLALEARRVLR
                     AASVFGEVCWEGGVGALLGSAMAAATVSEWLETLVEQEALAVRPNSRFPGEREFVFRH
                     ALLREGAYATLTEDDRRLGHRLAGEWLAQNGEADPMVLAGHFDRGGEGARAASYYLRA
                     AEQASLIADLDAMVTRAGLGLACDPPHELRNALLGMRCEASSYGLQMLDATMADAEEL
                     MRSAPRGSIPWAQAVNAYLSGMMMVGRIDDLLAAIALLREVDPDPEAMERMALAFLSG
                     LSMLDNLGRVPEATTLESRFLAVVRALGSDKPLAQFWWNIAVGMRASYAHEDPWTALE
                     HSDAIQAIHDAIGGEQIFLNMQLFRGLNLWHLGALTAAERTLNGIVVADEALGVASSL
                     RRLGLSWIRADLGALEEARSLADELSDYGRSQHNSFEEGRGRWVLAEVLRRIGDLDRA
                     DREIHAALGMSVPLEQPGVLATLSALRLAQGRAGEALAAAEDAVSRCTAMGGCGMFRG
                     VFARLAHAEALHATGAHDAARRAIAGARARLFAIVDKIPDPAYKASFLEHVPENARTL
                     ALAAAWLGDPAPPPAGD"
     gene            complement(3229..>3436)
                     /locus_tag="VF516_44820"
     CDS             complement(3229..>3436)
                     /locus_tag="VF516_44820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HEX8114959.1"
                     /translation="ATPNTQLFNMTGQPAISLPLSWNAAGLPIGTQWVAPFGREDLLL
                     RLASQLEAARPWATRRPRADATSA"
BASE COUNT          470 a         1224 c         1246 g          496 t
ORIGIN      
        1 cccgcgctgc ggcccagcga tggcgccaac ctggccgcgg cgctgacggc gctcgggccg
       61 cgggtgcgca gcgcgaccgt ggcgccgcat cgaaacccgg tgcggcgctc ggcgctcacg
      121 ggcggcgagc ggcggttgct atccgtggtg cgactggcga cgagcgatcg cgacacgctg
      181 gccgcggacg agctgcagcg ggccatccgg ccctacggcg gacacatgga gcagctcgct
      241 gacgggtcga cgatcgtggt gatcgaaccc gaccagcaga ccgactacat ggtggcaacc
      301 gatcaggccg cacaagcggc gcgctgcgcg ctcgcgatcc gtgcgctcat cgcggaccgt
      361 ccgatggcga tcgcgatggg gcgagtcgag tcgaccggcc agcggcccgg cggcgaggtg
      421 atcgaccggg cctcgcggct gctcgccgat tcgcgccagg ccgcggccgt ctcgatgccg
      481 atcgcgctcg atgagatcag cgccggcttg ctcgacgctc gattcgacgt ggtcgaggtc
      541 ggatccggcc tgatgctgcg cggcgagcgc gccctgatcc acggcgcccg gaccctgctc
      601 ggtcgcccga ccgcgtgtgt cggccgcgac tgggagctca acgcgctgat gtcgatcctc
      661 gacgagtgta tcgacgagcg cgaagcccgt gccgtgatcg tgaccgccgc ggccgggatg
      721 ggcaaatcaa ggctcgccgc cgagctcgtc acccgcatcc agcaacgcgg tgaaccggtc
      781 gcggtgtggg tcgcccgcgg cgactcgctg cgcgctggct cgacgctggg cctgctcgcc
      841 caggcattgc gcggcgtgct cggcctcccc ggtggcgagt cgctcgccgt acgccacgac
      901 aagatccgga cctgggtcgc cgagcacgcg gtcgagtccg agcgcaagcg ggtcgccgag
      961 ttcctcggcg agctgatcgg cgcgccgttt cccgccgacg gcgcggccgg cgcctcgctg
     1021 cgcgccgctc gccacgacgc tcagctgatg agcgagcaga tgcgcagggc ctggatggac
     1081 ttcctcgacg ccgaggccgc cgcccacccc gtcctgctcg tgctcgagga cttgcactgg
     1141 ggcgacttcg gcaccgtgcg gttcatcgac accgcgctgc gcgaccgcag ggagcggccg
     1201 tggatggtgc tcgcgctggc ccgccccgag gtgttccaga tgttccccaa gctgtgggcg
     1261 gagcgccaca acgtccagga gatccgcctc aaggagctcg gccgcaaggc cggtgagcgc
     1321 ctggtctgcc aggtgctcgg cgatcgcgtg ggccccgaca ccatcaagcg cctggtccgg
     1381 caagccgagg gcaacgcgtt ctatctcgag gagctgatcc gcgccgcggc cgagggcaag
     1441 gacggcgcgc tgcccgacac cgtgctcgcc atggtcgaga cccggctcgg ccggctcgcg
     1501 ctcgaggccc ggcgcgtgct gcgcgccgcc agcgtgttcg gtgaggtgtg ctgggagggc
     1561 ggcgtcggcg ccctgctcgg cagcgcgatg gcggcggcga cggtgagcga gtggctcgag
     1621 acgctcgtgg agcaagaggc gctggcggtg cggccgaaca gccggttccc cggcgaacgc
     1681 gaattcgtgt tccgccacgc gctgctccgc gagggcgcct acgccacgct gaccgaggac
     1741 gaccgccggc tcggccaccg gctcgccggc gaatggctgg cgcagaacgg cgaggctgat
     1801 cccatggtgc tcgccggcca cttcgatcgc ggcggcgagg gtgcgcgcgc cgcaagctac
     1861 tacctgcgcg ccgccgagca ggcatcgctc atcgccgacc tggacgccat ggtgacccgt
     1921 gcgggcctcg gcctggcctg cgatcctcca cacgagctcc gaaacgcgct gctcggcatg
     1981 cgctgcgagg catccagcta cggcctgcag atgctcgacg ccacgatggc cgacgccgag
     2041 gagctgatgc gctctgcccc gcgtggctcc attccctggg cccaggcggt gaatgcctac
     2101 ctctctggaa tgatgatggt gggcaggatc gacgacctgc tggcagccat cgcattgctc
     2161 cgggaggtcg atccggatcc cgaggcgatg gagaggatgg cactcgcctt cctctccggg
     2221 ctgtccatgc tggacaacct gggccgagtc cccgaggcaa cgacgctgga gagccggttc
     2281 ctcgcggtgg ttcgtgccct tggaagtgac aagccgcttg ctcagttctg gtggaacatt
     2341 gccgtgggga tgagagcgtc gtatgcccac gaggatccgt ggacggccct ggagcacagc
     2401 gatgccatcc aggcgatcca cgacgcgatc ggcggcgagc agatctttct gaacatgcag
     2461 ctgtttcgtg gcctgaacct ctggcacctc ggagctctca cggccgcgga gcggacgttg
     2521 aacggcatcg tggtggcaga cgaagcgctg ggcgtggcga gctcactgcg gcgcctgggc
     2581 ctgtcctgga tccgcgccga cctcggtgcg ctcgaggagg cgcgctccct ggcggacgag
     2641 ctgagcgact acggccgatc gcagcacaac tccttcgaag aaggtcgagg ccgctgggtg
     2701 ctcgccgagg tgcttcgccg catcggcgac cttgaccgcg ccgaccgcga gatccatgcc
     2761 gcgctcggca tgtcggtgcc gctcgagcag ccaggtgtcc tggcgacgct ctccgccctc
     2821 cgcctcgccc agggccgcgc cggtgaggca ctcgctgccg ccgaggacgc cgtgtcccgc
     2881 tgtaccgcca tgggtggatg cggcatgttc cgcggagtgt tcgctcgcct cgctcacgcc
     2941 gaggcgctcc acgcgaccgg cgcccacgac gccgcccggc gcgcgatcgc cggcgcgcgc
     3001 gcgcgcctgt tcgccatcgt cgacaagatc cccgatcccg cctacaaggc gagcttcctc
     3061 gagcacgtcc ccgagaacgc ccggaccctg gcgctcgcgg cagcatggct cggcgatcca
     3121 gcgccgcctc ccgccggcga ctgacacccg gaccgcggtc gtgtggtggg tccagctgcg
     3181 tatcctcgat cacggggacg gatccggccg gacaccggac acgcacggtc aggcgctggt
     3241 cgcgtccgct cgcggccggc gcgtggccca cggccgcgcc gcctcgagct ggctggccag
     3301 gcggagcagc aggtcctcgc gtccgaacgg cgcgacccac tgggtgccga tcggcaggcc
     3361 ggcggcgttc cacgacagcg ggagcgagat cgccggctgg ccggtcatgt tgaacagctg
     3421 ggtgttgggg gtcgcg
//
LOCUS       DASVOR010002444         3231 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_202334,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002444 DASVOR010000000
VERSION     DASVOR010002444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3231
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202334"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            353..1288
                     /locus_tag="VF516_44825"
     CDS             353..1288
                     /locus_tag="VF516_44825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114960.1"
                     /translation="MRVARWLVAAAVAVARPASADPPDPQVEKADELFAEAKALEATN
                     LPEACNKFAESLRYNPAAIGTLLNVALCDEKLGRIASAVAKFTEARDRSREQGLPQHL
                     RAAEDHLATLQPQVPHLAIRLTEQLSDTKVLVDDQVIAPNTLGDIAIDPGSHTITVSA
                     PDRLPHRATVTIERAEHKAIVIPALARSVTVTSSWRRIGQIATITGVAAFGTSIGLAL
                     YGRHIYLQQFPQHCGPNMSGGTSCDPIGQPKVDKARTYGNVATAVGGIGLAVAAAGAV
                     VWYLAPHSFQGTDAPPRVALDVTGDHVGVVAIGRF"
     gene            1493..1984
                     /locus_tag="VF516_44830"
     CDS             1493..1984
                     /locus_tag="VF516_44830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114961.1"
                     /translation="MEGLERDGEEYFVLDVFAWHVQELRDVFEHGHLGELQRLDPEQP
                     DRAWFGFRRNGTDYQIKVHRSGMAQITRRATGRLELSTEDQLARDRLDPEVVRQTEFP
                     GMLLGFLIGEPLSQAPNASRHVLTMRYNEDENRWRAYDGPQAVWIRNHALKIEPAFVA
                     AGA"
     gene            complement(2001..>3231)
                     /gene="acdA"
                     /locus_tag="VF516_44835"
     CDS             complement(2001..>3231)
                     /gene="acdA"
                     /locus_tag="VF516_44835"
                     /EC_number="3.13.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF042439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-sulfinopropanoyl-CoA desulfinase"
                     /protein_id="HEX8114962.1"
                     /translation="PGAFPPEPDMHPLTSEQRSLQLRARELAQGAFAATAADTDRTEQ
                     YPWANVALLRDAGFMGMTLPPRVGGQGRSYHDAVIVIEEMARACATMGRITVEANMGA
                     IGAIARYGTEPQLQLAAAAVLSGDKPAICISEPGAGSAASEMTTRADRRGDHYLLNGE
                     KYWITGGGVSRLHLIFARVFDDGVEQGIGGFLCLRDGDAPGELVVGKRLYAMGVRGIP
                     ETHLEFHDLPIHKSMLVVPPGGLARSFAALMAAYNAQRVGAGTVALGIAQGAFEEAVA
                     YARTRRQFGRPIAEFQGLQWMLADMSIQLDAARLLLHAAACSGTDFPDLAKAAQAKIF
                     AAEMANKVTSDALQIHGSAGYGRHNALERHVRDARMFTIAGGTAQVLRTQVASSVLGM
                     KLPQTRDGYLPADRKPR"
BASE COUNT          480 a         1165 c         1146 g          440 t
ORIGIN      
        1 gtggctcccg cggccacggc ctccgatccg cagcacaccg ccgcggcggc cggtgcgcca
       61 ccaccggccg ggccagcgac accaccggca ccttcgggaa cgcctgccgg caacgagccc
      121 cagtcccgac cgggaagcgc cggccgcgcg caggctgtcc cggcacggcc cggtccgaag
      181 ccatcccacc ccggccggca cggccgcgac atgatccccg gcgtggactg cacgctcaaa
      241 ggtgcattcg atgactccaa ggccctcccg ccctgtccga aagactgacg aggaccgggc
      301 gaggaaggac gatgatcgcg cggtgatcgc gggagctggt acaatccgcg ggatgcgtgt
      361 cgcccggtgg ctcgtcgcgg ctgcggtcgc ggtggcgcgc cccgcgagcg ccgacccgcc
      421 cgatccgcag gtcgagaagg ccgacgagct gttcgccgag gccaaggcgc tcgaagcgac
      481 caacctgccc gaggcgtgca acaagttcgc ggagtcgctg cgctacaacc ccgccgcgat
      541 cggcacgctg ctcaacgtcg cgctgtgcga cgagaagctc ggccgcatcg catcggcggt
      601 cgccaagttc accgaggccc gcgaccgttc ccgcgagcag ggactgcccc agcacctgcg
      661 cgccgccgag gaccacctcg cgacgctcca gccccaggtc ccgcacctcg cgatccggct
      721 caccgagcag ctatccgaca ccaaggtgct ggtcgacgat caggtgatcg ctcccaacac
      781 cctcggcgac atcgcgatcg atcccggctc gcacaccatc acggtcagcg cgcccgaccg
      841 cctgccccac cgcgccacgg tcacgatcga gcgcgccgag cacaaggcga tcgtcatccc
      901 cgcgctcgcc cgctcggtca ccgtgaccag ctcgtggcgc cggatcggcc agatcgccac
      961 catcaccggc gtagccgcgt tcggcaccag catcggcctg gcgctctacg gccgccacat
     1021 ctatctccag cagttccccc agcactgcgg acccaacatg agcggcggca cgtcgtgcga
     1081 ccccatcggc cagcccaagg tcgacaaggc ccggacctac ggcaacgtcg ccaccgcggt
     1141 cggcggcatc ggcctcgcgg tcgcggcggc cggcgcggtc gtctggtacc tcgcccccca
     1201 cagcttccag ggcaccgacg ccccgccccg cgtcgcgctc gacgtcaccg gcgaccacgt
     1261 cggcgtcgtc gccatcggcc ggttctgagc gcgcccacag cgtcgtgccc cagcggtcag
     1321 cgatgctgcg ggcgaatgcg tgctcgcgcg ggtcgcggcc gatccgctcg ctccgcgaga
     1381 tgtccggatt tcggacacaa ttccgatcgc aggcctggcc ccactgtgct gaaacgcgct
     1441 atcctggaga taacgagatg acgtcacccg actgctcacg ctgaccgagc tggtggaagg
     1501 cctggagcgc gatggtgagg agtacttcgt cctcgacgtc ttcgcatggc acgttcaaga
     1561 gctgagggac gtgttcgagc acggccacct cggcgagttg caacgcctcg accccgagca
     1621 acccgaccgc gcctggttcg gcttccggcg caacggaacc gactatcaga tcaaggtcca
     1681 ccggtccggc atggcccaga tcacccgccg ggcgaccggg cggttggagc tgtcaacaga
     1741 agatcagctc gcgcgggatc ggctcgaccc cgaggtggtg cgccagaccg agttccccgg
     1801 catgctcctg gggttcctca tcggagagcc cctgagccag gcgccgaacg cttcgcggca
     1861 tgtcctcacg atgcgctaca acgaggacga gaaccgctgg cgcgcctacg acggcccgca
     1921 agccgtctgg atccgcaatc acgcgttgaa gatcgagccc gcgttcgtgg cagccggcgc
     1981 gtaggcagtc gcgatgcagg tcagcgcggc ttgcggtcgg ccggcaggta gccgtcgcgg
     2041 gtctgcggca gcttcatgcc cagcacgctg ctcgccacct gggtgcgcag gacctgggcg
     2101 gtgccgccgg cgatcgtgaa catgcgggcg tcgcggacgt ggcgctcgag ggcgttgtgg
     2161 cggccgtagc cggccgagcc gtggatctgg agtgcgtcgc tggtgacctt gttggccatc
     2221 tcggcggcga agatcttggc ctgggcggcc ttcgcgaggt cggggaagtc ggtgccgctg
     2281 cacgcggcgg cgtggagcag gaggcgcgcg gcgtcgagct ggatcgacat gtcggcgagc
     2341 atccactgca ggccctggaa ctcggcgatc gggcggccga actggcggcg ggtcctggcg
     2401 taggccacgg cctcctcgaa ggcaccctgc gcgatgccga gggcgacggt gccggcgccg
     2461 acgcgctgcg cgttgtaggc ggccatcagc gcggcgaagc tcctggccag gccgccgggc
     2521 gggacgacga gcatcgactt gtggatcggc aggtcgtgga actcgaggtg ggtctcgggg
     2581 atgccgcgca cgcccatcgc gtacagccgc ttgccgacga ccagctcccc gggcgcgtcg
     2641 ccgtcgcgca ggcacaggaa gccgccgatg ccctgctcga cgccgtcgtc gaacacgcgc
     2701 gcgaagatca ggtggaggcg ggacacgccg ccgccggtga tccagtactt ctcgccgttg
     2761 aggaggtagt gatcgccgcg gcggtcggcg cgcgtggtca tctcgctggc ggcgctgccg
     2821 gcgccgggct ccgagatgca gatcgccggc ttgtcgccgg acagcacggc cgcggcggcg
     2881 agctggagct gcggctcggt gccgtagcgg gcgatcgcgc cgatcgcgcc catgttggcc
     2941 tcgacggtga tccggcccat ggtggcgcag gcgcgggcca tctcctcgat gacgatcacg
     3001 gcgtcgtggt agctccggcc ctggccgccg acgcgcgggg gcagggtcat gcccatgaag
     3061 ccggcgtcgc ggagcagcgc gacgttggcc caggggtact gctcggtgcg gtcggtgtcg
     3121 gcggcggtcg cggcgaacgc gccctgcgcg agctcgcgcg cgcgaagctg gagagagcgc
     3181 tgctcggagg tgagtggatg catgtcgggc tccgggggga aggctccggg g
//
LOCUS       DASVOR010002445         2916 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_56963,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002445 DASVOR010000000
VERSION     DASVOR010002445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56963"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VF516_44840"
     CDS             <1..244
                     /locus_tag="VF516_44840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114963.1"
                     /translation="PQSGGGGGAGGADGSGVIAADPTTSGDGGPGYPNAPFDGKDGAT
                     DGHGGGGGGGSPGAIRVVSTTDINRPNVSPPPVLLQ"
     gene            315..396
                     /locus_tag="VF516_44845"
     tRNA            315..396
                     /locus_tag="VF516_44845"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:349..351,aa:Leu,seq:gag)
     gene            complement(474..1742)
                     /locus_tag="VF516_44850"
     CDS             complement(474..1742)
                     /locus_tag="VF516_44850"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEX8114964.1"
                     /translation="MRQSGQKVNGPYLHRNQWRLIVRAADGSQEAIPFPTEAAARAHK
                     TRLLKEIAGRTVSEAVQEFIVEMRESGLRPGTVKVAEFRLRAFFQLDREVDGRTMPYA
                     ETGGRLEDLQPRACEQLYRAMVKTCAVDTHRNALAVAKSLGEWCVKQGWIATNPLAEI
                     TGVGQRNRGKKQLRIDEARRLVDVCIHEANQGDEAAVGVLTAFLLGLRASEVTDRVVR
                     DLDDEGRLLWIEFGKTRRSRRTLEVPALLRPYLLALAKGRAADAQLISRTVSQRSGKK
                     RDRYWLAHHVERLCTEAKVPVVCTQSLRGLHASVATDAGATSHVVASALGHSSPAVTH
                     AHYIDGATARRARTRRVVGKVAPEAALQAVKDEPATAITAATRAPRKPARRGSGMAAK
                     PALQAVAAPAPSTTSGTGEPTSAGEIVGNG"
     gene            complement(1863..>2916)
                     /locus_tag="VF516_44855"
     CDS             complement(1863..>2916)
                     /locus_tag="VF516_44855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114965.1"
                     /translation="SAKLMKIQMALSPDEQGDAMKILKGFPVEELRDLLTTFDLASVD
                     ECTDFLRRLILDWRKTQAARRTRATEPRAAGNVGDGDAVANAADPRAAYAASVEALRA
                     AQAEQARAAQEHAAQLASAIAAASSPGPGINVFSLLGLLPPYTASKLVHSNAALSPEE
                     QADAIQVLKSYSVEGMHELLTAFDANPVEECTTFLRSVIADWRAHRAAELARAKEVPP
                     SGSGGLSGAAGPISAAGSSGSSGAAGPSSAAGASSPSRAADPSGASGTTSSGVVVGSI
                     GASGAVGSSSPSRPADPSDATGPGAASHPVGSSDAAGSSDAVASSSAEGPSIAIAPSP
                     TSGAAGRGGSGSKKPR"
BASE COUNT          400 a         1071 c          955 g          490 t
ORIGIN      
        1 cccgcagtcc ggcggcggtg gtggcgccgg aggcgccgac ggctccggcg tgatcgccgc
       61 cgaccccacc acctccggcg acggcggccc gggctatcca aacgccccgt tcgacggcaa
      121 ggacggcgcc accgacggcc acggcggcgg gggcggcggc ggtagccccg gcgcgatccg
      181 cgtcgtctcc accaccgaca tcaaccgccc caacgtctcg ccgccgccgg tcctcctcca
      241 gtagcgcctc cggccatgcg gcttgacagg ccgcccctct ggcgctacca acgtccctcg
      301 cctgacaact tcatgcgcgg atggcggaat tggtagacgc gctagcttga ggtgctagtg
      361 ggtaacaccg tgggggttcg agtccccctt cgcgcacgag gaatcttcgc cccttacgcc
      421 accccagcga aaggggcgta agccttttcg gcttttgggt aacgcgcagg ccattacccg
      481 ttacccacga tctcgcccgc gctagtgggt tcgcccgtac cggaggtggt gctcggcgcg
      541 ggggctgcga ccgcctgcag ggccggcttg gctgccatgc cgctgccacg ccgagcaggc
      601 ttccgcggtg cccgggtggc tgcggtgatc gccgtggccg gttcatcctt gaccgcctgc
      661 agcgcggcct ccggggccac cttgccgacc acgcgccgcg tccgtgcgcg ccgggcggtc
      721 gcgccgtcga tgtagtgcgc gtgggtgacc gcgggcgagc tgtgcccgag ggccgaggcg
      781 accacgtgcg acgtcgcgcc ggcatcggtg gcgaccgacg catgcagccc gcgcagcgac
      841 tgcgtgcaga ccaccggcac cttcgcttcc gtgcacagcc gctcgacgtg gtgggccagc
      901 cagtagcgat cccgcttctt gccgctgcgc tgcgacaccg tgcggctgat cagctgcgca
      961 tccgcggcgc gccccttggc gagcgccaag aggtaggggc gcaacaacgc cggcacctcc
     1021 agcgtgcgcc gtgaccgtcg cgtcttgccg aactcgatcc acaagagtcg cccctcgtcg
     1081 tcgagatcgc gcaccacgcg atcggtcacc tccgacgccc gcaggccgag aaggaacgcg
     1141 gtcagcacac cgaccgcggc ctcatcgccc tggttcgcct cgtggatgca cacgtcgacg
     1201 agcctgcgtg cctcgtcgat ccgcagctgc ttcttgcccc ggttgcgctg accgacgccg
     1261 gtgatctcgg cgagcgggtt cgtcgcgatc cacccctgct tcacgcacca ttcgccgagc
     1321 gatttcgcca cggccagcgc gttgcggtgg gtgtcgacgg cacaggtctt caccatcgcg
     1381 cggtagagct gctcgcacgc ccggggctgc aggtcttcga gccgcccgcc ggtctcggcg
     1441 tacggcatcg tccggccgtc gacctcgcgg tccagctgga agaacgcgcg caaccgaaac
     1501 tcggcgacct tgaccgtccc tggacgcaag ccgctctcac gcatctcgac gatgaactcc
     1561 tgcaccgctt cgctcaccgt tcgcccggcg atctccttga gcagccgagt cttgtgagca
     1621 cgggccgccg cctccgtcgg aaacgggatc gcctcctggc taccgtccgc tgcccggacg
     1681 atgagccgcc actggttgcg atgtagatag ggtccattga ctttctgtcc tgactgccgc
     1741 atcgagtgtt ctcctcccga gcggcgattt cccgtcgggg atcatgccat ggcgcttcgc
     1801 gctttcaacg acggcgttga acgattcgaa gatttcttcg acgatgaacg ggcgagatct
     1861 gatcatcgcg gcttcttcga tccgctgccc ccccgacctg ccgccccgct cgtggggctc
     1921 ggagcgatcg caatgctcgg accttccgca gagcttgaag cgacggcatc gctcgaaccg
     1981 gccgcgtcgc tcgaaccgac gggatggctc gcagcgccgg gacctgtcgc atcgctcgga
     2041 tcggctggac ggctcgggct actcgaaccg accgcaccac tcgcgccgat cgagcccacg
     2101 acaacgccgg agctggtcgt gccgctcgca ccgctcggat cggctgcacg actcgggctg
     2161 ctggcaccgg ccgcgctgct cggaccggct gcgccgctcg acccgctcga accggccgcg
     2221 ctgatgggac cagccgcacc gctcaggccg ccgctgccgc tgggcggtac ctccttcgcg
     2281 cgcgccagct ccgccgcacg atgtgccctc cagtccgcga tcacgctgcg caggaaggtc
     2341 gtgcattcct cgacggggtt tgcgtcgaac gcggtgagta gctcgtgcat cccttcgacg
     2401 ctgtagctct tgagaacctg gatcgcgtcc gcttgctcct cgggcgacag cgctgcgttg
     2461 ctgtgcacga gcttcgatgc tgtatagggc gggagcagcc ccagcaggct gaacacgttg
     2521 atgcccggac caggagacga cgcggcggcg atggccgatg ccagctgcgc ggcgtgctcc
     2581 tgtgccgctc gcgcctgctc cgcctgcgcg gctcgcagcg cttcgacgga cgccgcgtac
     2641 gcggctcgcg gatcagcagc gtttgcgacc gcatccccgt cgccgacgtt gcctgccgct
     2701 cgtggttccg tggcgcgcgt tcgcctcgct gcctgcgtct tgcgccagtc aaggatgagg
     2761 cgtcgcagaa agtcggtgca ttcgtcgaca gacgcgaggt cgaaggtggt gaggaggtct
     2821 cgcagttcct cgacggggaa gcccttgagg atcttcatcg catccccctg ctcgtcgggc
     2881 gacagcgcca tctggatctt catcagcttc gcggac
//
LOCUS       DASVOR010002446         3687 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_553684,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002446 DASVOR010000000
VERSION     DASVOR010002446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3687
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553684"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..245
                     /locus_tag="VF516_44860"
     tRNA            172..245
                     /locus_tag="VF516_44860"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Arg,seq:tct)
     gene            276..1265
                     /locus_tag="VF516_44865"
     CDS             276..1265
                     /locus_tag="VF516_44865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEX8114966.1"
                     /translation="MASHVVDKQPITECLGERPRSSVEKPHTGGKAKQFFVRLYHELI
                     NDNIVDIGAMMAYYAILALFPMMVFVVTIAMLVLPDQTVMQGLAMATQAMPAAPREVI
                     STQVVSLMKQAHAGFAIGTFAFALWGASRGASSLMGALNAMFNKRETRPWWKRQVIAI
                     TVTACVAILAVVALGLLVVGPIAGHWLADRFGLGGQFDLVWGITRWVGAGLLVMVVWA
                     VAYKFLPNTDAPFRIFTPGALVGVLLWLGISYAFGVYLGYVNSYTTTYGALGGGIIFL
                     TWLWLSSIALLFGAEINDVLADLRKDRSPAAAALAHDNHDGREDAGLRDTPGA"
     gene            complement(1274..2626)
                     /locus_tag="VF516_44870"
     CDS             complement(1274..2626)
                     /locus_tag="VF516_44870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEX8114967.1"
                     /translation="MPSAAPLRILLRLAAPMILARASQSVITFADAIQVKHLGYQAIA
                     ATATGGLNVLLVVTLAWGTVFIVQSFVAQLAGRGDRDQAPRFAWYGLAIALGAGLIAA
                     ALIPLIGPALALTRYSPAVRDQMTDYMAIRMTSVFAVAGVEALGNWYGGLGNTWMQMI
                     AGLITMVAAVILNWVLIDGHLGAPAMGVNGAALASTIASFLGFAFVAIAFWRRWGGAP
                     AARATGLSLRELRRVLRFGLPNGINWFLEFAAFQLFVNVVLANLGDATVAALNVVIAV
                     NSIAFMPAFGLASAGAILAGQAIGRGERDAVWPQVKATLTCTMVWMGAIGATYAVFPD
                     RVLSLFASERSGELVAIGTTMLVISAAWQLTDAISMTLAETLRAAGDTAWTAGARVSL
                     AWLVFTPAAFLVVLRLAGGPVGAMLCLVGYLALLAVALAWRFRSGAWKRIELIEPALV
                     "
     gene            2674..>3687
                     /locus_tag="VF516_44875"
     CDS             2674..>3687
                     /locus_tag="VF516_44875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HEX8114968.1"
                     /translation="MAKAKQEILSQAGHQVAVSNPDKLYFPRAGITKLELVQYYLAVA
                     DGVLRGVARRPQILKRFVDGAEGEPFYQKRAPVRRPDWVEVATFTFPSGRHADEIVVN
                     NTAQLVYVVNLGCIDLNPHAVRAEDMDRPDELRIDLDPVPGVPWRQIIDVARVAREVL
                     DEHGLIGWPKTSGSRGVHIWVRIRPAWPFTQVRRAALAFAREVARRAPEIATAKWWKE
                     ERRGVFLDYNQNARDRTTASAYSVRPTPDARVSMPLSWDALFGCDPLDFTLRTVPAMV
                     AAHGDAHAGIDAAVGSIESLLELADAQGPERPKKAAKVKLPVITIGQAKQKPDAIAGL
                     ERWK"
BASE COUNT          575 a         1277 c         1334 g          501 t
ORIGIN      
        1 cgctcggcgg atgggcacgg actgccggca ccggcgttcg gtgcgtgacc gcgggcggcc
       61 ggcccgggcg gcgcccgggg gaaggcgatc ggcgccgcgg aggggcttgc cggggttgag
      121 agatccgcca acctggggca aagtgcgaga ccatccctcc gatccacatc cgcgcccata
      181 gttcagttgg atagaacaac cgccttctaa gcggtaggtc gcaggttcga ctcctgctgg
      241 gcgcgctggc acaatcgctg ctccaccgga ggggcgtggc cagtcacgtc gtggacaagc
      301 agccgatcac cgagtgcctc ggcgagcgcc ctcgctcgtc cgtggagaag ccgcacaccg
      361 gtggcaaggc gaagcagttc ttcgtgcggc tctaccacga gctgatcaac gacaacatcg
      421 tcgacatcgg ggcgatgatg gcctactacg cgatcctggc gctgttcccg atgatggtgt
      481 tcgtggtgac gatcgcgatg ctcgtgctgc ctgaccagac cgtgatgcag ggcctggcga
      541 tggcgaccca ggcgatgccg gcggcgccgc gcgaggtgat ctcgacccag gtggtgtcgc
      601 tgatgaagca ggcccacgcg gggttcgcga tcgggacctt cgccttcgcg ctgtggggcg
      661 catcgcgcgg ggcgagctcg ctgatgggcg cgctcaacgc gatgttcaac aagcgcgaga
      721 cccggccgtg gtggaagcgc caggtcatcg cgatcaccgt gacggcatgc gtcgcgatcc
      781 tcgccgtcgt ggcgctcggc ttgctggtgg tcggtccgat cgccggacac tggctcgccg
      841 accggttcgg gctcggcggg cagttcgacc tcgtgtgggg catcacccgc tgggtcggcg
      901 ccggcctgct ggtgatggtg gtgtgggcgg tggcctacaa gttcctgccc aacaccgacg
      961 cgccgttccg gatcttcacg ccgggggcgc tcgtcggggt gctgctctgg ctgggcatca
     1021 gctacgcctt cggggtctat ctcggctacg tcaacagcta caccacgacg tacggcgcgc
     1081 tgggcggcgg catcatcttc ctgacctggc tgtggctatc gagcatcgcg ctcctgttcg
     1141 gcgccgagat caacgacgtg ctcgccgatc tgcgcaagga caggagcccg gccgcggcgg
     1201 cgctggccca cgacaaccac gacggccgtg aggacgccgg cctgcgcgac acgcctggcg
     1261 cgtgacaccc gcgtcacacc agcgcgggct cgatcagctc gatccgcttc cacgcgccgg
     1321 accggaaccg ccaggccagc gcgacggcga gcagggcgag gtagccgacc aggcacagca
     1381 tcgcgccgac cggcccgccg gcgagccgga gcacgaccag gaacgccgcc ggtgtgaaca
     1441 ccagccacgc gaggctcacg cgggccccgg cggtccacgc cgtatcgccg gccgcgcgca
     1501 gggtctcggc gagcgtcatg ctgatcgcat cggtcagctg ccaggccgcg ctgatcacca
     1561 gcatcgtggt gccgatcgcg accagctcgc ccgagcgctc ggacgcgaac agcgacagca
     1621 cgcggtccgg gaacaccgcg taggtcgcgc cgatcgcgcc catccacacc atcgtgcagg
     1681 tgagcgtggc cttgacctgc ggccacaccg cgtcgcgctc gccgcgaccg atcgcctggc
     1741 cggcgaggat cgcgccggcc gacgccaggc cgaacgcggg catgaacgcg atcgagttga
     1801 ccgcgatcac cacgttgagc gcggcgacgg tggcgtcgcc gaggttggcg agcacgacgt
     1861 tgacgaacag ctggaacgcc gcgaactcga ggaaccagtt gatcccgttg ggcagcccga
     1921 accgcagcac gcggcgcagc tcgcgcagcg acaggcccgt ggcgcgcgcg gcaggggcac
     1981 cgccccagcg ccgccagaac gcgatcgcga cgaacgcgaa gccgaggaag ctcgcgatcg
     2041 tgctggcgag cgcggcgccg ttgacgccca tcgccggcgc gccgaggtgg ccgtcgatca
     2101 gcacccagtt gaggatcacc gcggcgacca tggtgatcag gccggcgatc atctgcatcc
     2161 aggtgttgcc gagcccgccg taccagttgc cgagcgcctc gacccccgcg accgcgaaca
     2221 ccgacgtcat ccggatcgcc atgtagtccg tcatctgatc gcggacggcg ggcgagtagc
     2281 gggtgagcgc cagcgccggg ccgatcagcg ggatcagcgc cgcggcgatc agcccggcac
     2341 cgagcgcgat cgccaggccg taccaggcga accgcggcgc ctggtcgcga tcgccccgcc
     2401 cggcgagctg cgcgacgaag ctctggacga tgaacaccgt gccccacgcg agggtcacga
     2461 cgagcagcac gttgagcccg ccggtcgcgg tcgcggcgat cgcctggtac ccgaggtgct
     2521 tgacctggat cgcgtcggcg aacgtgatca ccgactggct ggcgcgcgcc aggatcatcg
     2581 gcgcggccag ccggagaagg atgcggagcg gggcggccga cggcacggtg ggctaccatg
     2641 tggcgcggcg gccgatcgtg caaggatctc ccggtggcaa aggccaagca ggagatcctg
     2701 tcgcaggcgg ggcaccaggt cgcagtgtcc aaccccgaca agctgtactt cccgcgcgcc
     2761 gggatcacca agctcgagct ggtgcagtac tacctcgcgg tcgccgacgg cgtgctgcgt
     2821 ggcgtcgcgc gccggccgca gatcctgaag cggttcgtcg acggcgccga gggcgagccg
     2881 ttctaccaga agcgcgcgcc ggtgcggcgg ccggactggg tcgaggtcgc gacgttcacg
     2941 ttcccgtcgg ggcggcacgc cgacgagatc gtggtcaaca acaccgcgca gctggtctac
     3001 gtggtcaacc tcggctgcat cgatctcaac ccgcacgcgg tgcgcgccga ggacatggac
     3061 cggcccgacg agctgcggat cgacctcgat ccggtccccg gcgtcccgtg gcgccagatc
     3121 atcgatgtcg cgcgggtcgc gcgcgaggtg ctcgacgagc acggcctgat cggctggccc
     3181 aagacctcgg gctcgcgcgg cgtccacatc tgggtccgga tccggccggc gtggccgttc
     3241 acgcaggtcc gccgcgccgc gctcgcgttc gcccgcgagg tcgcgcgccg cgcgcccgag
     3301 atcgcgaccg cgaagtggtg gaaggaggag cgccgcgggg tgttcctcga ctacaaccag
     3361 aacgcgcgcg atcgcacgac cgcgagcgcc tactcggtgc ggccgacgcc ggatgcccgg
     3421 gtgtcgatgc cgctgtcgtg ggacgccctg ttcgggtgcg atccgctcga cttcacgctg
     3481 cgcaccgtgc cggcgatggt cgcggcgcac ggcgacgcgc acgccgggat cgatgccgcg
     3541 gtcggctcga tcgagtcgct gctcgagctc gccgatgcgc agggacccga gcggccgaag
     3601 aaagcggcga aggtgaagtt gccggtgatc acgatcggcc aggccaagca aaagccggat
     3661 gcgatcgccg ggctcgaacg atggaag
//
LOCUS       DASVOR010002447         1638 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_143718,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002447 DASVOR010000000
VERSION     DASVOR010002447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143718"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /gene="clpX"
                     /locus_tag="VF516_44880"
     CDS             complement(<1..654)
                     /gene="clpX"
                     /locus_tag="VF516_44880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEX8114969.1"
                     /translation="MPIEKRDGASLTCSFCGKAQKEVKKLIAGPTVYICDECIGLCND
                     IIAEEIEKEDQSYGTSSIPKPAQIKKVLDEYVIGQDRAKKILAVAVHNHYKRIDHKQT
                     GQSDEDVELQKSNILLLGPTGSGKTLLAQTLARILNVPFAIADATNLTEAGYVGEDVE
                     NIIVSLLQNADHDIERAQRGIVYIDEIDKIARKSDNPSITRDVSGEGVQQALLKIIEG
                     "
     gene            complement(925..1545)
                     /gene="clpP"
                     /locus_tag="VF516_44885"
     CDS             complement(925..1545)
                     /gene="clpP"
                     /locus_tag="VF516_44885"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEX8114970.1"
                     /translation="MTPTMNYLVPTVIEQTHRGERGWDIFSRLLKDRIIFLGTPINDD
                     VANVVIAQLLFLDSEEPEKDIMLYINSPGGHVSAGLAIYDTMQYLHCDVATICMGQAA
                     SMGSFLLAAGTKGKRYALPHARVMIHQPLAGFQGQATDIDIHAREILKTRDTLNELYA
                     KHTGQPSDKIKHDTERDNFMSSSQAKEYGIIDEVLVTAKKKPDDKK"
BASE COUNT          220 a          507 c          576 g          335 t
ORIGIN      
        1 gccctcgatg atcttgagca gcgcctgctg cacgccctcg ccggacacgt cgcgggtgat
       61 cgacgggttg tcgctcttgc gggcgatctt gtcgatctcg tcgatgtaga cgatgccgcg
      121 ctgggcgcgc tcgatgtcgt ggtcggcgtt ctgcagcagc gagacgatga tgttctcgac
      181 gtcctcgccg acgtagccgg cctcggtgag gttggtggcg tcggcgatcg cgaacggcac
      241 gttgaggatc cgcgccaggg tctgggcgag cagggtcttg cccgagccgg tcggcccgag
      301 caagaggatg ttcgacttct ggagctcgac gtcctcgtcg ctctggccgg tctgcttgtg
      361 atcgatccgc ttgtagtggt tgtggaccgc gaccgcgagg atcttcttgg cgcggtcctg
      421 accgatcacg tactcgtcga ggactttctt gatctgggcc ggcttgggga tcgagctcgt
      481 gccgtagctc tggtcttcct tctcgatctc ctcggcgatg atgtcgttgc acagcccgat
      541 gcactcgtca cagatgtaca cggtcgggcc ggcgatcagc ttcttgactt ccttctgggc
      601 cttgccgcag aacgagcatg tcagggacgc accgtcacgc ttctcgatgg gcatcgggtc
      661 gaagcggagc gtaccgcggg gtcgatgtgg ggaccagtcg gccggcgctc gccggcggtc
      721 caaccgcctg aacgaccggg gtgcaagatt tcaggtagct gccgatacat acatcggccc
      781 gtgtcgagcc ccgctcagga tctcacttcg cccggtgtgg gcggggtggc ggcgttcgag
      841 ccgccggccg gatcccggtg gccaggatcg gtggccagga tcggccgcgc ccgcccccgg
      901 cggcccggcc gcgtggcgct cacctcactt cttgtcgtcg ggcttcttct tcgcggtgac
      961 cagcacctcg tcgatgatcc cgtactcctt ggcctgcgaa gacgacatga agttgtcgcg
     1021 ctcggtgtcg tgcttgatct tgtcggacgg ctggccggtg tgcttggcgt acagctcgtt
     1081 gagcgtgtcg cgcgtcttca ggatctcgcg ggcgtggatg tcgatgtcgg tcgcctggcc
     1141 ctggaagccg gcgagcggct ggtggatcat cacgcgcgcg tgaggcagcg cgtagcgctt
     1201 gcccttggtg ccggcggcga gcaggaagct gcccatcgac gccgcctgcc ccatgcagat
     1261 cgtcgcgacg tcgcagtgca ggtactgcat ggtgtcgtag atcgccaggc cggccgacac
     1321 gtgtccgccg ggcgagttga tgtacagcat gatgtccttc tcgggctcct cggagtcgag
     1381 gaacaacagc tgcgcgatca ccacgttggc gacatcgtcg ttgatcggcg tgccgaggaa
     1441 gatgatgcgg tccttgagca ggcgggagaa gatgtcccag ccgcgctcgc cgcggtgagt
     1501 ctgctcgatg accgtcggga cgaggtagtt catcgtgggc gtcatggggt accgccaact
     1561 ctacgggcgc ccgctcgtgc gtgcaacgcg cccggcggtg aggcccgcgt ccgatcgcct
     1621 ggccgttcgg gtaggacc
//
LOCUS       DASVOR010002448         1632 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_319329,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002448 DASVOR010000000
VERSION     DASVOR010002448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319329"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VF516_44890"
     CDS             <1..426
                     /locus_tag="VF516_44890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114971.1"
                     /translation="AVPAGALAAAATVALVVSSRPPPALALNVEVKHSAVFRGDDASV
                     GDIAHVVVSGGKGGRAIWVYREDVQLVLRCPGDPACRVSDASTEVDLPLTSAGTYTVV
                     ALTAASNLPAPTGSYGADTAAARDAGLDVRQYRLPVSRQ"
     gene            complement(456..1439)
                     /locus_tag="VF516_44895"
     CDS             complement(456..1439)
                     /locus_tag="VF516_44895"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8114972.1"
                     /translation="MTNLSCTFGRTLLLGSLSLAGCANNSGNTNVACAPLTKTAAAVV
                     PPAWKGGTVFTIVMENHSRGEILGNSAAPYINQLAGDSAVAEGYHDSFVHPSEPNYFW
                     MVAGQNFGVLDDGDPASHHLASTSHIADQLEQAGLSWRSYQESMGSPCGLTSHGRYAA
                     KHNPFAYFDDINGWDGQAFHPEQRCNAHVVDYSQLDADIANHALPNYVFITPNLDNDM
                     HDGSVGQGDSWLARELPKLLATDSYKSGGVIFLLWDEGGGTPASDDPPFIVISPNAAH
                     GTKSQTDYDTSSYLLTVQNILGLQPLPCAAAADRSTTAAMTDLFTVPLTGT"
BASE COUNT          228 a          532 c          597 g          275 t
ORIGIN      
        1 gcggtgcccg ctggggcgct cgcggcagcc gctacggtcg cgctggtcgt gtcgagccgg
       61 ccgccgcctg cgctcgccct gaacgtcgag gtcaagcaca gcgcggtgtt ccgcggcgat
      121 gacgccagcg tcggtgacat cgcacacgtc gtcgtgtccg gcggcaaggg cgggcgcgcg
      181 atctgggtct atcgcgagga cgtccagctc gtgctgcgct gtcccggcga tccggcgtgc
      241 cgggtctccg atgcgtcgac cgaggtcgat ctaccgctga cctccgcggg gacctacacc
      301 gtggtcgcgc tgaccgcggc ctccaacctg cccgcgccga cggggagcta cggcgccgac
      361 acggccgccg ccagggacgc cggcctcgac gtccggcagt accgcctgcc cgtgagccgg
      421 cagtagccgc cggcgccgga gccgccggtc gtacgtcagg tgccggtgag cggcacggtg
      481 aacagatcgg tcatcgccgc ggtcgtgctg cgatcggcgg cggccgcgca gggcagcggc
      541 tggagcccga ggatgttctg cacggtcagc aggtacgagc tggtgtcgta gtcggtctgc
      601 gacttcgtgc cgtgcgccgc gttgggcgag atcacgatga acggcggatc gtcggacgcc
      661 ggcgtgccgc cgccctcgtc ccatagcagg aagatcacgc cgccgctctt gtagctgtcg
      721 gtcgcgagca gcttgggcag ctcgcgggcg agccagctgt cgccctggcc gaccgagccg
      781 tcgtgcatgt cgttgtcgag gttcggcgtg atgaacacgt agttcggcag cgcgtggttg
      841 gcgatgtcgg cgtcgagctg gctgtagtcg accacgtggg cgttgcagcg ctgctcggga
      901 tggaacgcct ggccgtccca gccgttgatg tcgtcgaagt aagcgaacgg gttgtgcttg
      961 gccgcgtagc ggccgtgcga ggtcagcccg cacggactgc ccatgctctc ctggtacgac
     1021 ctccagctca gcccggcctg ctcgagctgg tcggcgatgt gcgaggtcga ggccaggtgg
     1081 tgcgatgccg gatcgccgtc gtcgagcacc ccgaagttct ggccggcgac catccagaag
     1141 tagttcggct cgctcgggtg gacgaacgag tcgtgatagc cctcggccac ggcgctgtcg
     1201 ccggcgagct ggttgatgta cggcgccgcc gagttgccga ggatctcgcc ccggctgtgg
     1261 ttctccatga cgatcgtgaa caccgtcccg cccttccagg ccggcggcac caccgccgcc
     1321 gccgtcttcg tcaatggggc acaggcgaca ttggtgttgc ccgagttgtt ggcgcagccg
     1381 gcgagcgaca gcgagcccag gaggagagtt cgcccgaagg tgcaggagag attcgtcatg
     1441 cgaaacaacc ttgttctcga cattcctgtt ggcaagcgaa gagtatgcgt tccggggtgc
     1501 aatcgtcgac gcaaatcgtg cgtcctgtgg aatcgcgtgg aagcgagctc ggcgattcga
     1561 gatcgaaggt ccgccatgcg cagggcgatc gcgctgcctg cccgatccgc gaggtggcgc
     1621 cgtgcgggac cc
//
LOCUS       DASVOR010002449         1968 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_494815,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002449 DASVOR010000000
VERSION     DASVOR010002449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1968
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494815"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VF516_44900"
     CDS             complement(<1..378)
                     /locus_tag="VF516_44900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114973.1"
                     /translation="MQDRAHSPDLAAAPDLGPLPAPAADLGLATALLQHLQAIGRLTP
                     NDIAAALQRYPQLREAVLHVVAAHWGTRGVQALVDAEAQYGAPAAATAPAIVTGSAAT
                     QAAAPAHAASVHQVAERDALNAVI"
     gene            complement(511..894)
                     /locus_tag="VF516_44905"
     CDS             complement(511..894)
                     /locus_tag="VF516_44905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114974.1"
                     /translation="MTPSPNLPANVQGSLGDLAKPKMDASLAQANAKMTEAETKRDTD
                     RSKAVSDAEAKVKQAHADADKQQQSKVADARTQIANHQADPMSQRSGLRCPGNRRRGG
                     NRVLGGAFVTLEDTHVAPPAEAAPG"
     gene            complement(986..1573)
                     /locus_tag="VF516_44910"
     CDS             complement(986..1573)
                     /locus_tag="VF516_44910"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEX8114975.1"
                     /translation="MTRVPATAPTAAELSTERLVLRAWRDADLAPFAALSADPRVMEH
                     FAAPLSRAESDAMVERIREHFAGHGFGLWAIEVPGVADFIGFVGLVHVPFTAPFTPAV
                     EVGWRLAAEHWGRGYATEGARRALEVGFEQLRLEQIVSITMPANQRSRHVMEKLGMRR
                     SPADDFDHPRLPEGHPLRRHVLYRLSRTDWSNQRC"
     gene            1749..>1968
                     /locus_tag="VF516_44915"
     CDS             1749..>1968
                     /locus_tag="VF516_44915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114976.1"
                     /translation="MISTVALWGCGGPVVFQGQSTLPVTGTPPPAVAEAKPPPRVEVR
                     DNKIEIHDKIQFDYDKATIRPESFGLMNE"
BASE COUNT          279 a          675 c          725 g          289 t
ORIGIN      
        1 gatcaccgcg ttgagggcgt cgcgctcggc gacctggtgc acagacgcgg cgtgcgccgg
       61 agcggcggct tgggtggcgg cggatccagt gacgatcgcg ggcgctgtcg ccgccgcggg
      121 cgcgccgtat tgggcctcag cgtcgaccag cgcctgcaca ccgcgggtgc cccagtgggc
      181 ggcgaccacg tgaagaacgg cctcgcggag ctgcgggtac cgctggagcg cggcggcgat
      241 gtcgttcggg gtcagccggc cgatggcctg aaggtgctga aggagggccg tggcgagacc
      301 gagatcagcg gcgggcgcgg gcagcgggcc gaggtcgggc gccgcagcaa gatctgggga
      361 gtgagctcgg tcctgcacgc cgacgtccct tcctgcggga gatggtcggc gcagtccgat
      421 aacaagccaa gggccggacc gtcgggcgag cgtgaggctg cacgggcagt caccctcggt
      481 gagcgcctac gacacgcgtc ggctgggcgg ctaccccggc gcggcctcgg caggtggcgc
      541 aacgtgtgtg tcctccaggg tcacgaacgc cccgccgagc accctgtttc cgccgcgcct
      601 ccgatttccg ggacaacgga ggccacttct ctgagacatg gggtcggcct ggtgattggc
      661 aatctgggtc cgggcgtcgg cgaccttgga ctgctgctgc ttgtcggcgt cggcgtgggc
      721 ctgcttgacc ttggcctcgg cgtcgctcac cgccttgctg cggtcggtgt cgcgcttggt
      781 ctcggcctcg gtcatcttgg cgttggcctg ggcgaggctg gcatccatct tgggcttggc
      841 gagatcaccg agcgagccct ggacgttggc cggcaggttc ggcgacggtg tcacacgctg
      901 tcgcatccag atccgacgat tgccaatgcc tgccacgtgc cagaacgggc atcgcggcgc
      961 tcggcgttgt ttgcgactgc gaggatcagc agcgctgatt cgaccagtcg gtgcgcgaca
     1021 gccggtacag cacgtggcgg cgcagcggat ggccctcggg gagccgcggg tgatcgaagt
     1081 cgtcggccgg cgagcgccgc atgccgagct tctccatcac atggcgcgat cgctggttgg
     1141 ccggcatcgt gatcgacacg atctgctcga gccggagctg ctcgaagccg acctccagcg
     1201 cccggcgcgc gccctcggtc gcgtagccgc gcccccagtg ctcggcggcc agccgccagc
     1261 cgacctcgac ggccggcgtg aacggcgccg tgaacgggac gtggaccaac ccgacgaagc
     1321 cgatgaagtc ggcgacgccc gggacctcga tcgcccacag gccgaagccg tggccggcga
     1381 agtgctcgcg gatccgctcg accatcgcgt cgctctcggc ccgcgacagc ggcgcggcga
     1441 agtgctccat cacccgcgga tcggcgctga gcgccgcgaa cggcgcgagg tcggcgtcgc
     1501 gccacgcgcg cagcacgagg cgctcggtcg acagctcggc agcggtgggc gctgtggccg
     1561 gaacgcgcgt catccgtcgc cggcatccta ccccgaccgc gcgatcttgc gttgcagatc
     1621 ggccggttag cccgcccgcc gccagaattt gccggatccg tgaacacggc gcacgccccg
     1681 ccgtcctcgc gatcgttggc cccgtccacg gatccgggcc cgaggagaga ccgatgcgag
     1741 catggttcgt gatcagcacc gttgcgctct ggggatgcgg tggccccgtc gtgttccagg
     1801 gccagagcac gctgcccgtc accgggacgc cgccgccggc cgtcgccgag gccaagccgc
     1861 cgccgcgcgt cgaggtgcgc gacaacaaga tcgagatcca cgacaagatc cagttcgact
     1921 acgacaaggc gacgatccgg cccgagtcgt tcggcctgat gaacgaga
//
LOCUS       DASVOR010002450         1728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_319353,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002450 DASVOR010000000
VERSION     DASVOR010002450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1728
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_319353"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            104..1354
                     /locus_tag="VF516_44920"
     CDS             104..1354
                     /locus_tag="VF516_44920"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8114977.1"
                     /translation="MTDSPHRHRSHPPPWLFVFAGTPYGVVGQFTGLVMPYATEHAGE
                     QLGSIGWYNALLFLPTFLLFLYAPIVDIGPRRKHWLVIVSAIGAGCLIASFQMKLPEQ
                     IPAYLAFAFAAQLISGLVGSCAGGLMATAMPDELRGRAGGWYNIGNLSGGGLFAAIAI
                     AMIGHGVDPRVIGGVVAVMMVLPAVALLVIEEPPRPHIAPRVVFRQTLREVGDVLASR
                     SGLTGIALSLSPVGTAALANYFSGMSTPYGVGADVVAVVTGAGTVGLNAVGALIGGTL
                     CDRFNRRVLYLLSGALTAVCGIAMALSPRTASTYVLGVTAYALITGLCFAAFTAYVLE
                     TIGRGDRTAATKYSLFTAASNFAITYVGWIDTRFNERHGVEGVVASDAALNLIGVAVL
                     ALVFWRLGSFGRWRHPAEAAEAKA"
     gene            complement(1361..>1728)
                     /locus_tag="VF516_44925"
     CDS             complement(1361..>1728)
                     /locus_tag="VF516_44925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114978.1"
                     /translation="RGAFKKDSGRAEAAPAPRAPSGGYAPPSDVEGDDVAMPEPQRRE
                     RLGLGTEFGEQRTSAASYTRFVRAGGRPVAIAELRYNDTAGLIALGIPVQPLPDGDEI
                     MLRETANPFPGDRFAQPPR"
BASE COUNT          202 a          621 c          633 g          272 t
ORIGIN      
        1 gtgcccccgg agagcgcccg gcaacgcggc ggagccccgc aggctcgcgt ctcgtgcgcg
       61 aggcatcgcc gccgccgcgc caggtcggct attcatggat cgcgtgaccg actcgccgca
      121 tcgtcatcgc agccatccgc cgccgtggct gttcgtgttc gccggcacgc cctacggcgt
      181 cgtcggccag ttcaccggcc tggtgatgcc ctacgcgacc gagcacgccg gcgagcagct
      241 cggatcgatc ggctggtaca acgcgctgct gttcttgccg accttcctgc tgttcctcta
      301 tgcaccgatc gtcgacatcg ggccgcgccg caagcactgg ctggtgatcg tgtcggcgat
      361 cggcgcgggc tgcctgatcg cgagcttcca gatgaagctg cccgagcaga tccccgcata
      421 cctcgcgttc gcgttcgccg cacagctgat ctccggcctg gtcggcagct gcgccggcgg
      481 gctgatggcg acggcgatgc ccgacgagct gcgcggcagg gccggcggct ggtacaacat
      541 cggcaacctg tcgggcggcg gactgttcgc ggcgatcgcg atcgcgatga tcggccacgg
      601 cgtcgacccg cgcgtgatcg gcggcgtggt cgccgtgatg atggtgctgc cggccgtcgc
      661 cctgctggtg atcgaggagc cgccccggcc gcacatcgcg ccgcgggtgg tgtttcgcca
      721 gacgctgcgc gaggtcggcg acgtgctcgc cagccgctcc gggctgaccg gcatcgcgct
      781 gtcgctgtcg ccggtcggca ctgccgcgct ggcgaactac ttctcgggca tgtcgacgcc
      841 gtacggggtc ggcgccgacg tcgtcgcggt ggtcaccggg gccggcaccg tcgggctgaa
      901 cgccgtgggg gcgctgatcg gcggcactct gtgcgaccgg ttcaaccgcc gcgtgctgta
      961 cctgctgtcg ggggcgctga ccgcggtgtg cggcatcgcc atggcgctgt cgccgcgcac
     1021 ggcatcgacc tacgtcctcg gcgtcacggc ctacgcgctg atcaccgggc tctgcttcgc
     1081 ggcgttcacc gcgtacgtcc tggagaccat cggcaggggc gaccggaccg cggcgaccaa
     1141 gtactcgctg ttcaccgcgg cctccaactt cgcgatcacg tacgtcggct ggatcgacac
     1201 ccggttcaac gagcgacacg gcgtcgaggg cgtggtcgcg agcgacgcgg cgctcaacct
     1261 gatcggcgtc gccgtcctcg cgctggtgtt ctggcggctc ggctcgttcg gcaggtggcg
     1321 ccatcccgcc gaggccgccg aggccaaagc ctgaccgccg tcagcgcggc ggctgcgcga
     1381 agcggtcgcc ggggaacggg ttggcggtct cgcgcagcat gatctcgtcg ccgtccggca
     1441 gcggctggac cgggatgccg agcgcgatca gaccggcggt gtcgttgtag cgcagctcgg
     1501 cgatcgcgac cggccggccg ccggcgcgga cgaaccgggt gtagctcgcg gcggaggtgc
     1561 gctgctcgcc gaactcggtg ccgaggccca gccgctcgcg gcgctgcggc tcgggcatcg
     1621 cgacgtcatc gccctcgacg tccgacggcg gtgcgtagcc accggacggc gcgcgcggcg
     1681 ccggtgctgc ctcggcgcgc ccggagtcct tcttgaaggc cccgcgcg
//
LOCUS       DASVOR010002451         1848 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_435443,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002451 DASVOR010000000
VERSION     DASVOR010002451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1848
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435443"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..379
                     /locus_tag="VF516_44930"
     CDS             74..379
                     /locus_tag="VF516_44930"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEX8114979.1"
                     /translation="MARRRKFSDEPFGPTIERLMGETGTTYRGLAERTKLSAGYLNHL
                     VHGNRPVPSDKVVAELARALGVEPEHFREYRLRVITRRLEAMPDLIDRLYKRLGNEA"
     gene            486..1187
                     /locus_tag="VF516_44935"
     CDS             486..1187
                     /locus_tag="VF516_44935"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEX8114980.1"
                     /translation="MAFGGERTAVLVDPFPLWLDAVESVLAKNGVRVIGRASQAEQGI
                     ALCEELGPQILVAEPAAGGIVCIREATKRVPGLKAIALASSGDQDQVQAAFAAGAVAY
                     VVKTADAADVGVAVRLVFSPSLFLPGLVPAVAANGNGVGTAPPDPAVELLTKREREVL
                     RLVAEGHSNSQLARMLWVTEQTVKFHLSNIYRKLDVANRTEASRWAQLHGLLEQATAG
                     SVAPERSNGELRATR"
     gene            1266..1340
                     /locus_tag="VF516_44940"
     tRNA            1266..1340
                     /locus_tag="VF516_44940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Pro,seq:ggg)
     gene            complement(1389..>1848)
                     /locus_tag="VF516_44945"
     CDS             complement(1389..>1848)
                     /locus_tag="VF516_44945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114981.1"
                     /translation="QRRNVVAALAAARELPHLSLEDALRRAGTRAWVPAGCSATSKED
                     CATPLGVRTMPAEIDKTYGHLLPDALDRTRRALDSFIAAGSDELAEVEQLRVQYHLPR
                     TNPPNAMRPMSAMIKPSKMLQIKATTMPTITRIPPSVIPPYLRSPMPASP"
BASE COUNT          298 a          574 c          664 g          312 t
ORIGIN      
        1 ggtggacgtg cgcgaccgcc ggagcggcga ggagcgacgt gtaccgatag tcgagctggg
       61 gagcatcgtc tagatggcgc gccggcggaa gttcagcgac gagccgttcg gcccgaccat
      121 cgagcggctg atgggcgaga ccggcacgac ctaccgcggc ctcgccgaga ggacgaagct
      181 ctcggccggc tacctgaacc acctggtgca cggcaaccgg cccgtcccgt cggacaaggt
      241 cgtggcggag ctcgccaggg cgctcggcgt ggagcccgag cacttccgcg agtaccggct
      301 gcgcgtgatc acgcgccggc tggaggcgat gccggacctg atcgaccgcc tgtacaagcg
      361 gctgggcaac gaggcctgaa aggcacgctg ctgtgccgta gccgtgcaac gtctttacag
      421 gacggccgaa tttgagtatc tcctagtaag gacgaaagtc ccagcgggga gaaagggctg
      481 tcactgtggc gttcggcggg gaaagaactg cggtactggt cgaccccttt ccgttgtggc
      541 tggacgcggt cgagagcgtg ctcgcgaaga acggcgtgcg cgtcatcggc cgtgcttcgc
      601 aggcggagca gggcatcgca ctctgcgagg agctagggcc gcagatcctc gttgccgagc
      661 cggcggccgg gggcatcgtt tgcatccgtg aggcgacgaa gcgagttccc gggctgaagg
      721 cgatcgccct ggcctcctcc ggagatcagg atcaggtaca ggcggcgttc gccgcgggtg
      781 ccgtggcgta cgtcgtcaag actgcggatg cggccgacgt cggcgtggcg gtgcgtctgg
      841 tgttctcgcc gtcgctgttc ttgcctggcc tcgttccggc cgtcgcggca aacggcaacg
      901 gcgttggtac agcgcccccc gaccccgcgg tggagttgct caccaaacgt gagcgcgagg
      961 ttctccggct cgttgccgag ggccactcca acagccagct cgcgcggatg ctctgggtca
     1021 ccgagcaaac ggtgaagttt cacctgtcga acatctaccg gaagctcgac gtggcgaatc
     1081 ggactgaggc cagtcgttgg gcgcagctgc atgggttgct cgagcaggcc accgccggct
     1141 cggtggcgcc agagcgctca aacggcgagc tgcgggcgac acggtaagcc tggctgcgca
     1201 gaaggcccgg ctccgcggcg cggcagcgtc cgcggatcgg ggcctgagct agagtgcaag
     1261 gccgacgggg cgtggcgcag cctggttagc gcgctagtct gggggactag aggtcccgag
     1321 ttcaaatctc ggcgccccgc ttgaaaccag ggcgagagca gtgcccacta ggtgcccaaa
     1381 cgtcctcatc acggagaggc aggcatggga gaacgtaggt acggagggat cacgcttggc
     1441 gggatcctcg tgatcgtcgg catcgtcgtc gccttgatct ggagcatctt gctcggcttg
     1501 atcatcgcgc tcatcggcct cattgcgttc ggcgggttcg tccgcggtaa gtggtactga
     1561 acacgtaact gctcgacctc agctagttcg tcgctgcccg ccgcgatgaa ggaatcgagc
     1621 gcccgccgcg tccggtcgag cgcgtccggc agcaagtggc cataggtctt gtcgatctca
     1681 gctggcatcg ttctcactcc caaaggtgtc gcgcagtctt ccttcgaggt agcgctgcag
     1741 ccagcgggca cccacgcgcg cgtgccggcg cgtcttagcg cgtcctccag gctcaggtgt
     1801 gggagctcgc gcgcagcggc tagggctgcg accacgtttc gccgttgg
//
LOCUS       DASVOR010002452         2851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_494868,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002452 DASVOR010000000
VERSION     DASVOR010002452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494868"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VF516_44950"
     CDS             complement(<1..602)
                     /locus_tag="VF516_44950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8114982.1"
                     /translation="MRALAVVTALVLLAGAARADTIDANIHQLKDSSYKVRLAAVLAL
                     SKSHEPRAVIALADALASPHLKDDEPTIRRVAALALGKMIDARTADDARELGLDALAD
                     AAINDPDLKVRATALRAEQDLAGLRRQNQKKKDPASDPRTNNRADKPGVFVNVDSATD
                     QSKVAPSDAGERLARVVKKTVERTGYATSWPGPLPTQTELA"
     gene            686..1396
                     /locus_tag="VF516_44955"
     CDS             686..1396
                     /locus_tag="VF516_44955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEX8114983.1"
                     /translation="MSLVDSVRVLGAMARITAPTLVDVARGSVKRTAIDERTRWFGRR
                     VVELLDIQLTATGAEHVPRRAVIYMSNHQSHLDIPVLYATLPSPTIRMLAKAELFRIP
                     LWGRGLRAAEFIEVDRGNHTRAVQSIEHAARLVRDGVSIWVAPEGTRSIDGRIGKLKK
                     GGFHLALATGTPIVPVAIRGTIGILPRGAKVMRTHQLVSVQIGPPIDVAGRDMPGLMA
                     QVSEFLVKNVENVTGNGR"
     gene            1415..>2851
                     /locus_tag="VF516_44960"
     CDS             1415..>2851
                     /locus_tag="VF516_44960"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEX8114984.1"
                     /translation="MTWVVPLALGGFAILLMAVFTMMSARKRHRAALAPATGVGPRVL
                     VHDINDDRCTGCDACVAVCPTNVLDLVENKSRVLRFHDCIQCEACMFACPTEALVMFP
                     EGGQPPPLKVPEMDEHFQTAVPGQYLIGEVAGKPLVKNAANLGRAVVEHMLATGMRPG
                     GLGGGDNVVDVAIVGSGPGGLSAALTCIQRGLSYVVLEKEQVIASTIARYPKGKLVMA
                     EPYDATNLSLLPVFDSAKEQLIPIWRELIERVGMTIHQGESVETVARAGDGAFDVRTT
                     VATYRAQRVVLSIGTRGKPRTLQVPGENLPKVFSLLEDPDEWRGRAVLVVGGGDSACE
                     AALALADAGAKVMISYRGKGFNRAAPKNKQAIESYAGQGRIKAKLGSQVVAFEPDSVT
                     LALSDGSQKRYPNDGAFVLIGADPPVQWLEKMGVRFVQRPHQYQLGKTDDIVRRFVAR
                     AGECPEDAARAAAQVLGGSVGVEPSLSAS"
BASE COUNT          411 a          928 c         1078 g          434 t
ORIGIN      
        1 gcgagctcgg tctgggtcgg cagcgggccg ggccagctgg tcgcgtagcc ggtgcgctcg
       61 acggtcttct tgacgacccg ggcgagccgc tcgccggcat cgctcggcgc gaccttggac
      121 tgatcggtgg cgctgtcgac gttgacgaac acgccgggct tgtcggcgcg gttgttggtc
      181 cgcgggtcgg acgccggatc cttcttcttc tggttctggc ggcgcaggcc ggcgaggtcc
      241 tgctcggcgc gcagcgcggt cgcgcggacc ttgaggtcgg ggtcgttgat cgcggcgtcg
      301 gcgagcgcgt cgaggccgag ctcgcgggcg tcgtcggcgg tgcgcgcgtc gatcatcttg
      361 ccgagcgcga gcgcggcgac ccggcggatc gtcggctcgt cgtccttgag atgcgggctg
      421 gcgagggcgt cggcgagcgc gatcacggcg cgcggctcgt gcgacttgga cagcgcgagg
      481 acggcggcga gccggacctt gtagctcgag tccttgagct ggtgaatgtt ggcgtcgatc
      541 gtgtcggcgc gtgccgctcc ggccaacaga accaaggcgg tgaccaccgc cagcgcgcgc
      601 atggccgaag aaggtaacac gccggagcga tcgcggaatc gggggacggc ccacgacatc
      661 tcccacctcg gcgtagaatg cacgcatgtc tctggtcgac agcgtgcgcg tgctcggagc
      721 gatggcgcga atcacggcac ccacgctcgt ggacgtcgcg cggggatcgg tgaagcgcac
      781 agcgatcgat gagcggacgc ggtggttcgg ccggcgagtg gtcgaactgc tcgacatcca
      841 gctgaccgcg accggcgccg agcacgtgcc gcggcgggcg gtcatctaca tgtcgaacca
      901 ccagagccac ctcgacatcc cggtgctgta cgcgacgctg ccgtcgccga ccatccgcat
      961 gctcgccaag gccgagctgt tccggatccc gctgtggggt cgcgggttgc gcgcggccga
     1021 gttcatcgag gtcgaccgcg gaaaccacac ccgcgcggtg cagtcgatcg agcacgcggc
     1081 gcggctggtg cgggacgggg tgagcatctg ggtcgctccg gagggcacgc ggtcgatcga
     1141 cggccggatc ggcaagctga agaagggtgg gttccacctg gcgctcgcca ccgggacgcc
     1201 gatcgtgccg gttgcgatcc gcggcacgat cgggatcctg ccgcgcggcg ccaaggtgat
     1261 gcggacccac caactggtgt cagttcagat cggtcccccg atcgacgtgg cagggcgtga
     1321 catgcccggt ctgatggctc aagtcagtga attcctggtg aagaacgtcg agaacgtcac
     1381 gggcaacggc cggtaaatca ggtattcttc tgtcgtgacc tgggtggtcc cactggcgct
     1441 cgggggcttc gccatcctct tgatggcggt gttcacgatg atgtccgccc gcaagcgcca
     1501 ccgcgcggcg ctggccccgg ccacgggggt gggcccgcgg gtgctggttc acgacatcaa
     1561 cgacgatcgc tgcacgggct gcgacgcgtg cgtcgcggtg tgcccgacca acgtgctgga
     1621 tctcgtcgag aacaagagcc gcgtgctgcg gttccacgac tgcatccagt gtgaggcgtg
     1681 catgttcgcg tgcccgaccg aggcgctggt gatgttcccc gagggcggcc agccgccgcc
     1741 gctcaaggtc cccgagatgg acgagcactt ccagaccgcg gtgccgggcc agtacctgat
     1801 cggcgaggtc gccggcaagc cgctggtcaa gaacgccgcg aacctcgggc gcgccgtggt
     1861 cgagcacatg ctggcgaccg ggatgcggcc ggggggcctc ggcggcggcg acaacgttgt
     1921 cgatgtcgcg atcgtcggct cgggcccggg cgggctgtcc gccgcgctga cgtgcatcca
     1981 gcgcgggctg tcgtacgtcg tgctggagaa ggagcaggtg atcgcgtcga cgatcgctcg
     2041 ctaccccaag ggcaagctgg tgatggccga gccgtacgac gcgaccaacc tgtcgctgct
     2101 gccggtgttc gactcggcca aggagcagtt gatcccgatc tggcgcgagc tgatcgagcg
     2161 ggtcgggatg acgatccatc aaggcgagtc ggtcgagacc gtcgcgcgcg ccggcgacgg
     2221 cgcgttcgac gtgcgcacca cggtggcgac ctatcgcgcg cagcgcgtcg tgctgtcgat
     2281 cggcacgcgc ggcaagccgc gcacgctgca ggtccccggc gagaacctgc ccaaggtgtt
     2341 cagcctgctg gaggatcccg acgagtggcg cggccgcgcg gtgctggtgg tcggcggcgg
     2401 cgactcggcg tgcgaggcgg cgctcgcgct ggccgacgcc ggcgccaagg tgatgatctc
     2461 gtaccgcggc aagggcttca accgcgccgc gcccaagaac aagcaggcga tcgagagcta
     2521 cgccggccag ggccggatca aggccaagct cggctcgcag gtcgtcgcgt tcgagcccga
     2581 ctcggtgacg ctcgcgctga gcgatggctc gcagaagcgc taccccaacg acggtgcgtt
     2641 cgtgctgatc ggcgccgatc cgccggtcca gtggctcgag aagatgggcg tccggttcgt
     2701 ccagcggccg caccagtacc agctgggcaa gaccgacgac atcgtgcgcc ggttcgtggc
     2761 gcgcgcgggc gagtgccccg aggacgccgc gcgggccgcg gcccaggtgc tgggcggcag
     2821 cgtcggggtc gagccgtcgc tcagcgcgag c
//
LOCUS       DASVOR010002453         1752 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_85714,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002453 DASVOR010000000
VERSION     DASVOR010002453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1752
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85714"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VF516_44965"
     CDS             complement(<1..544)
                     /locus_tag="VF516_44965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TCP-1/cpn60 chaperonin family protein"
                     /protein_id="HEX8114985.1"
                     /translation="MAAKELIFSSGARGEIAKGLNLLANAVRVTLGPRGRNVVIEKSW
                     GAPTVTKDGVTVAKEIELENKFQNMGAQMVKEVASKTSDVAGDGTTTATVLAQSIYNE
                     GAKLVAAGLNPMDLKRGIEAGVHTVVEQIKAMATPTKGKEDIAQVGTISANGDTEIGE
                     MIAEAMKKVGKEGVITVEEAK"
     gene            complement(592..879)
                     /gene="groES"
                     /locus_tag="VF516_44970"
     CDS             complement(592..879)
                     /gene="groES"
                     /locus_tag="VF516_44970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEX8114986.1"
                     /translation="MKIRPLHDRILVKRLEEETKTSGGLFIPDSAKEKPIQAKVIAVG
                     AGKRDKDGKIIALEVKAGDRVLFSKYSGTEVKIDGEEHLIMREDDLLAVLD"
     gene            1123..>1752
                     /locus_tag="VF516_44975"
     CDS             1123..>1752
                     /locus_tag="VF516_44975"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEX8114987.1"
                     /translation="MALTDYEKYIRTEELLALQKAPDALTCHDELQFQVVHQAHELYM
                     KLIEHELRFLCSLLEAGEVPRAVTTLGRVSRIQRVLLQSIDLLDTMAPTDYMTIRTGL
                     GRGSGQESPGFRTMLRLPGDLVWPTVASFLDRQGVALRQIYEAPHDHHVLFQLCEGLV
                     DYDQLLQTWRYRHLLLVYRIIGTGTPSLKGKHSDLLQHGMKQRFFPKLWD"
BASE COUNT          254 a          574 c          569 g          355 t
ORIGIN      
        1 tcttggcctc ctcgaccgtg atcacgcctt ccttgccgac cttcttcatc gcctcggcga
       61 tcatctcgcc gatctcggtg tcgccgttgg cgctgatcgt gccgacctgg gcgatgtcct
      121 ccttgccctt ggtcggggtc gccatcgcct tgatctgctc gaccacggtg tggacgccgg
      181 cctcgatgcc gcgcttgagg tccatcgggt tgagcccggc ggcgaccagc ttggcgccct
      241 cgttgtagat cgactgggcg agcacggtgg cggtggtggt gccgtcgccg gcgacgtcgg
      301 aggtcttcga cgcgacctcc ttcaccatct gcgcgcccat gttctggaac ttgttctcga
      361 gctcgatctc cttggcgacg gtgacgccat ccttggtcac cgtgggagcg ccccacgact
      421 tctcgatcac gacgttgcgg ccgcgcggtc cgagcgtgac cctcacggcg ttggcgagca
      481 ggttgaggcc cttggcgatc tcaccgcggg cgccggacga aaaaatcagt tctttggcag
      541 ccatggtcct gtccttctgg atctatgtgc gctggtggtg gaggggtggg atcagtcgag
      601 cacggcgagg aggtcgtcct cgcgcatgat caggtgctcc tcgccgtcga tcttgacctc
      661 ggtgccgctg tacttgctga acagcacgcg gtcgccggcc ttgacctcga gcgcgatgat
      721 cttgccgtcc ttgtcgcgct tgcccgcgcc gacggcgatc accttggcct ggatcggctt
      781 ctccttcgcc gagtcgggaa tgaacaggcc accggaggtc ttggtctcct cttcgagacg
      841 cttgaccagg attcgatcat gtagcggacg gatcttcatt gctctcactc cagttggtgc
      901 ttcgccggta ccctatagac atcaggggcg cgcttggcaa cccctcgcgc cgagtgctaa
      961 caaaaatatt gaaattcagg cgatttcagt attttatgct actggctgaa acggggatgg
     1021 cctgcccggt ttggcagtcc ggggcctcga ctgccaggtg tcccgggtgc gcgggcggac
     1081 ctgcgcgggg ggccaccggg tcgtccgtgg taggacgttc gcgtggcgct caccgactac
     1141 gagaagtaca tccgcaccga ggagctgctc gcgttgcaga aggcgcccga cgcgctgacc
     1201 tgtcacgacg agctccagtt ccaggtcgtg caccaggctc acgagctgta catgaagctg
     1261 atcgagcacg agctgcggtt cctgtgcagc ctgctggagg ccggcgaggt gccgcgcgcg
     1321 gtgaccacgc tcggccgggt atcgcggatc cagcgcgtgc tgctccagtc gatcgatctg
     1381 ctcgacacca tggcgccgac cgactacatg acgatccgca ccgggctcgg ccgcggatcg
     1441 ggccaggagt cgccggggtt ccggaccatg ctgcggctgc ccggcgatct ggtgtggccg
     1501 acggtcgcga gcttcctcga ccgccagggc gtcgcgctgc gccagatcta cgaggcgccg
     1561 cacgatcacc acgtgctgtt ccagctgtgc gaggggctgg tcgactacga tcagctgctc
     1621 cagacctggc gctaccgcca cctcctgctg gtctaccgga tcatcggcac cggcacgccg
     1681 tcgctcaagg gcaagcacag cgacctgctg cagcacggca tgaagcagcg gttcttcccc
     1741 aagctgtggg ac
//
LOCUS       DASVOR010002454         1526 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_28792,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002454 DASVOR010000000
VERSION     DASVOR010002454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28792"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1481
                     /locus_tag="VF516_44980"
     CDS             <1..1481
                     /locus_tag="VF516_44980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114988.1"
                     /translation="DTWERVSPDLTTNDPEKQKQEESGGVTVDNSSAEMHTTIYSISE
                     SPKNPSVIWVGTDDGNVQLSRDGARTWTNAAGNVPGLPRASWVSWVEASRFDPAVAYA
                     AFDRHTFGDMAPWVFRTTDYGKTWTRIVGPDQGVRGYAHTIKEDTVKKDLLFVGTELG
                     LWISLDGGARWAQYRGGEFPDGVAVREIQVHPRDSDLVIATHGRGIWIVDDITPLRAL
                     GDDALQKSTAFLPIRDVQQRMPAQGGWPEGDATFTGDNAPGGATITYYLRSRHVYGPI
                     KLEVLDAGSKVIDTIAPSKRRGINRVSWAMRVKPPKVPRAAQIAFSSSQGPRVPPGTY
                     TVRLTRGTEVVETKLAIGLDRRAPYTALDRKAQFEAVMKTHGVFGDMSRLTTRIDAAR
                     EAVHDRLKGLPDTDPLAGKLRALGERLEGARKLIVATKEGGAITGEERIREHLDSLYG
                     ALNGWEGRPAKYQLDAIDALRRELGDVAAAVDLIVGKDARAR"
BASE COUNT          260 a          527 c          555 g          184 t
ORIGIN      
        1 gcgacacctg ggagcgggtg tcgcccgacc tcacgaccaa cgatccggag aagcagaagc
       61 aggaggagtc gggcggcgtc acggtcgaca actcgtcggc cgagatgcac acgacgatct
      121 actcgatcag cgagtcgccg aagaacccca gcgtgatctg ggtcggcacc gacgacggca
      181 acgtgcagct gtcgcgcgat ggcgccagga cctggaccaa cgcagccggc aacgtccccg
      241 gcctgcccag ggcgtcgtgg gtcagctggg tcgaggccag ccggttcgat ccggcggtcg
      301 cgtacgcggc gttcgaccgc cacacgttcg gcgacatggc gccgtgggtg ttccggacca
      361 ccgactacgg caagacctgg acccggatcg tcggaccgga tcagggcgtg cgcggctacg
      421 cccacacgat caaggaggac acggtcaaga aggacctgct gttcgtcggc accgagctcg
      481 ggctgtggat ctcgctcgac ggcggtgcgc ggtgggcgca gtaccggggc ggcgagttcc
      541 ccgacggcgt cgcggtccgc gagatccagg ttcacccgcg cgacagcgac ctggtgatcg
      601 cgacccacgg ccgcgggatc tggatcgtcg acgacatcac gccgctgcgc gcgctcggcg
      661 acgacgcgct gcagaagtcg accgcgttcc tgccgatccg cgacgtccag cagcggatgc
      721 cggcccaggg cggctggccg gagggcgacg ccacgttcac cggcgacaac gcgccgggcg
      781 gcgcgacgat cacctactac ctgcggtcgc gccacgtcta cggcccgatc aagctggagg
      841 tgctcgacgc cggcagcaag gtgatcgaca cgatcgcccc gagcaagcgg cgcgggatca
      901 accgggtcag ctgggccatg cgggtcaagc cgcccaaggt gccgcgcgcc gcgcagatcg
      961 cgttctcgtc gtcgcagggt ccgcgggtcc cgccgggcac gtacaccgtg cggctgaccc
     1021 gcggcaccga ggtcgtcgag accaagctcg cgatcgggct cgatcgccgc gcgccgtaca
     1081 ccgcgctgga ccgcaaggcg caattcgaag cggtgatgaa gacgcacggc gtgttcggcg
     1141 acatgtccag gctcaccacc cggatcgacg ccgcgcgcga agcggtgcac gaccggctga
     1201 agggcctgcc cgacaccgac ccgctggccg gtaagctgcg cgcgctgggc gagcggctgg
     1261 agggagcgcg caagctgatc gtcgccacca aggagggcgg tgcgatcacc ggcgaggagc
     1321 ggatccgcga gcacctcgac tcgctgtacg gggcgctcaa cggctgggag gggcggccgg
     1381 cgaagtacca gctcgacgcg atcgacgcgc tgcgccgcga gctcggcgac gtggcggccg
     1441 cggtcgacct gatcgtcggc aaggatgcgc gcgctcgatg acgagctcaa gcagcacaag
     1501 ctcgcgccgc taccgcagat cagcgc
//
LOCUS       DASVOR010002455         2777 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_553838,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002455 DASVOR010000000
VERSION     DASVOR010002455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553838"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="VF516_44985"
     CDS             complement(<1..384)
                     /locus_tag="VF516_44985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114989.1"
                     /translation="MANVDDMSADPARGILEPEHASVEPLGSVEPAARPSATRRSARP
                     ASGGNAAKPSRAKAKDTPATSGAAAKLDRKPARARTTREPADPDDDRAMTSGPPTIEP
                     SGPRTAALRQGLPRAGAYRASVVTRS"
     gene            complement(645..1031)
                     /locus_tag="VF516_44990"
     CDS             complement(645..1031)
                     /locus_tag="VF516_44990"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8114990.1"
                     /translation="MAVETKRPGVAFHRWNDMPRERVTDEIDRRVITGERMMLAHVYL
                     KKGSIVPRHSHENEQLTYILEGALRFKIGPEGSEVIVVRAGEVLVIPSNVPHEAEALE
                     DTLDVDVFDPPRQDWLDGTDHYFRRK"
     gene            complement(1046..1834)
                     /locus_tag="VF516_44995"
     CDS             complement(1046..1834)
                     /locus_tag="VF516_44995"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8114991.1"
                     /translation="MDLGLRGRVAMVAAGSKGLGRAIATALVAEGARVSICARSAGPL
                     AETEQALRAAGGDVLALAVDVSRPDELARWHEATVERLGAPALLVTNTGGPPPGLFEE
                     LNEDAWRAGIEGALMNVVRMSRLVLPAMRAAKYGRIVHLTSFVAKQPMALLTISSTLR
                     AGLSALTKTMGSQVAADGITVNAVLPGHFATDRQIQLSDIKARQHGITRAAWEDRVLA
                     EIPARRFGRPEEFGDLVAFLLSERAGYINGTSLQIDGGLTAGTF"
     gene            1982..>2777
                     /locus_tag="VF516_45000"
     CDS             1982..>2777
                     /locus_tag="VF516_45000"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease H3TH domain-containing
                     protein"
                     /protein_id="HEX8114992.1"
                     /translation="MADRTLVASATNLLARGYLVVPTDRRSPGGEPVNALFAVARAIH
                     RVMAFKEPSRAVAVLDATVDTTGWPPLLAAQLPALRGLLETLGLRVAEAPGEVHVVAS
                     YAHAALGGGHDVIVVGVDKRFAQLVSDRLWWYDANKDARYTPEMVQKRFLVPPAQVAE
                     WLALVGDDDALPGVAGIGAKGAAGLLESHGSIEHALASAATIPGRPGNVLRSSPDAVR
                     TELARARLDLHRPLPVALDELAYAPPGAAALNELYDRLGFAELLAPD"
BASE COUNT          361 a         1053 c          963 g          400 t
ORIGIN      
        1 cgatcgcgtc acgacggacg cacggtacgc gccggccctg ggcagcccct ggcgcagcgc
       61 cgcggtccgc ggtccggacg gttcgatcgt cggtgggccc gacgtcatcg cgcgatcgtc
      121 gtcgggatcc gccggttcgc gggtggtgcg cgcgcgagcc ggcttgcgat cgagcttcgc
      181 cgcggccccg ctcgtcgccg gcgtgtcctt ggccttcgcc cgcgacggct tcgcggcatt
      241 gccaccggac gccgggcgcg ccgatcgcct cgtcgcgctc ggcctcgccg ccggctcgac
      301 cgatccgagc ggctcgacgg acgcgtgctc cggctccagg atcccccgcg ccggatcggc
      361 ggacatatcg tcgacgtttg ccatggatga tcgccgagca cccggtgcgg acccgagccg
      421 caaccacgtg actcccgggc tcatcatata gggggcgggt cgcgcgtgcg cgacggcgtg
      481 ggccggagtc acgacgtctc acgttcgccg tacgccgccg cgaacgatca catcggcgcg
      541 actcggtggc gatcacgcca ctccagcaca cccgcgcgca ctcgccggga ttgaaatgtt
      601 tctcaatccg gagcggcgca gcacggatta ccggccgcgc cggctcactt ccggcggaag
      661 tagtgatcgg tgccgtcgag ccagtcctgg cgcggcggat cgaacacgtc gacgtcgagc
      721 gtgtcctcga gcgcctcggc ctcgtgcggg acgttggacg ggatcaccag cacctcgccc
      781 gcgcgcacga cgatcacctc ggagccctcg ggaccgatct tgaaccgcag cgcaccctcg
      841 aggatgtagg tcagctgctc gttctcgtgg gagtggcgcg gcacgatgct gcccttcttc
      901 aggtagacgt gggcgagcat catccgctcg ccggtgatca cccggcgatc gatctcgtcg
      961 gtcacccgct cccggggcat gtcgttccag cgatgaaacg caacgcccgg cctcttcgtc
     1021 tcaactgcca tcggagtctc cttggtcaaa aggttccggc cgtcaaaccg ccgtcgatct
     1081 gcagcgaggt cccgttgatg tatcccgcgc gctccgacag caggaacgcc accagatcgc
     1141 cgaactcctc ggggcgcccg aaccgccgcg ccgggatctc ggccagcacg cggtcctccc
     1201 acgcggcgcg cgtgatgccg tgctggcggg ccttgatgtc gctgagctgg atctgccggt
     1261 cggtcgcgaa gtggccgggc agcaccgcgt tgaccgtgat gccgtcggcc gcgacctggc
     1321 tgcccatcgt cttggtcagc gccgacagcc cggcgcgcag cgtcgacgag atcgtgagca
     1381 gcgccatcgg ctgcttggcg acgaacgacg tcaggtgcac gatccggccg tacttcgcgg
     1441 cgcgcatcgc cggcaggacc agccggctca tccgcaccac gttcatcagc gcgccttcga
     1501 tcccggcgcg ccaggcgtcc tcgttcagct cctcgaacag ccccggcggc ggcccgccgg
     1561 tgttggtgac cagcagcgcg ggagcgccga gccgctcgac ggtggcttcg tgccagcgcg
     1621 ccagctcgtc gggccggctg acatcgaccg ccagggccag cacgtcgccg cccgcggcgc
     1681 gcagcgcctg ctcggtctcg gcgagcgggc cggccgagcg cgcgcagatc gagacccggg
     1741 caccctcggc gaccagcgcc gtggcgatcg cccgtccgag tcccttcgag ccagcggcga
     1801 ccatggcgac gcggccgcgg agtccaagat ccatggcgcg cactctacac ccggcgagaa
     1861 cgccgcgggt cacccggccg ccattcgctt cccgctcctc ggtgatcgcc cgcgctgcga
     1921 gcgattccta cgcctgtagt accagtcaac ggccgcgatc tcggtaagct gtgcgcaacc
     1981 gatggccgac cgcacgctcg tcgcctccgc gaccaacctg ctggcgcggg gatacctggt
     2041 ggtgccgacc gatcggcgat cgccgggcgg cgagccggtc aacgcgctgt tcgcggtcgc
     2101 gcgcgcgatc caccgcgtca tggcgttcaa ggagccgtcg cgcgcggtcg ccgtgctcga
     2161 tgccaccgtc gacaccaccg gctggccgcc gctgctcgcg gcccagctgc cggccctgcg
     2221 cggcctgctc gagaccctcg ggctgcgcgt cgccgaggcg cccggcgagg tccacgtcgt
     2281 cgcgtcgtac gcgcacgcgg cgctgggcgg cggccacgac gtgatcgtcg tcggcgtcga
     2341 caagcggttc gcccagctcg tgtccgatcg gctgtggtgg tacgacgcca acaaggacgc
     2401 gcgctacacg ccggagatgg tccagaagcg gttcctggtc ccgccggcgc aggtcgccga
     2461 gtggctcgcg ctggtcggcg acgacgacgc gctgcccggc gtcgccggga tcggggccaa
     2521 gggcgcggcc ggcctgctcg agagccacgg ctcgatcgag cacgcgctgg ccagcgccgc
     2581 gaccatcccc ggccggcccg gcaacgtgct gcgatcgtcg cccgacgcgg tgcgcaccga
     2641 gctggcccgc gcgcggctcg acctgcaccg gccgctgccg gtggcgctcg acgagctggc
     2701 ctacgcgccg cccggcgccg ccgcgctcaa cgagctctac gaccggctcg ggttcgccga
     2761 gctgctcgcg cccgacg
//
LOCUS       DASVOR010002456         6962 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_494922,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002456 DASVOR010000000
VERSION     DASVOR010002456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6962
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494922"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VF516_45005"
     CDS             complement(<1..1434)
                     /locus_tag="VF516_45005"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8114993.1"
                     /translation="MADVPGSAGPFDPNETGTGLTTLFEGDWATVRRLRKGKLVVVSG
                     ADQGKSVEIAKPRVTGGRSIISDLVVQDKAVSGTHFEVSARDDGYRLRDLNSRNGIFV
                     GDLRIREVFLRPGTVFRIGHTNIQFQTLEDVVEIELSKKDRFDAMLGASPAMREIFAH
                     LEKVAPSDLTCLITGETGTGKEMVARALHNASGRKSRPFVVLDCGSIPRELIESTLFG
                     HEKGSFTGAVAQHVGCFEQASGGTIFLDEIGELDIMLQPKLLRVLEQREIKRVGGDRT
                     IKVDVRVLAATNRDLREEVNKGTFREDLYFRLSVVHVELPPLRERREDIPGLATHFLR
                     EIGARRGMAMAWSQDALAALVTHSWPGNVREMRNVVERAAALSDGPTITRTDLVFGRE
                     MGPSVVVSHDLAQAGAQAAQRAAASMSGVELPPQTGAATFDPALLKAGLGFKGAKQTV
                     VDAFEIAYLSALMARNDGNITRSAQEAG"
     gene            complement(1471..2199)
                     /locus_tag="VF516_45010"
     CDS             complement(1471..2199)
                     /locus_tag="VF516_45010"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEX8114994.1"
                     /translation="MSGAQRSRFSGMRITRGALIVLGLEVGLSLVWLALDPATQKWFS
                     DYLMATPANVFVHGRVWTLATGVFLEPKLLALLLHGLVLWMFVPTLERFWGTARFYRF
                     VAITSLVATAAGCGFAYALGDLNAMFGGLDPFIYAAIVAFGIVYARQPVQFFGVLPLT
                     GRQLMYGILAFLALLIVLSQRWADGVAYAAAMLAAAILTSKRWSPALAWKRWRIARAR
                     ARLTVMSGGKARSSPPRDEHKWLN"
     gene            complement(2252..3562)
                     /locus_tag="VF516_45015"
     CDS             complement(2252..3562)
                     /locus_tag="VF516_45015"
                     /inference="COORDINATES: protein motif:HMM:NF015980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpqB family beta-propeller domain-containing
                     protein"
                     /protein_id="HEX8114995.1"
                     /translation="MFSIRPQHVVLAALAAAITSLVSLGGPARADDPSGGVVIDVKGA
                     NRTLYPIAVPASQEGDAAAAREIAQVASFDLSVAGVFKVLDPAGFLADLKAEGLIIDP
                     QKWKDVGAFGVIKYRATASEIELRLYEISKGTTSVLSKVYPRKGDTRTIVHRFCNEVV
                     KYYTGEPGFFGSKIAFTAKGKTASAIYAMDFDGASAYAVSNNSSQNILPAWAPSGGQI
                     AYTSFMRNNPDLYVAPAGGGRPNKLSSQRGMNTGAAWSPDGKQLAITLSKDGNPEIYV
                     ISAADGSIVRRITNDRAIDTSPAWSPDGSQLAFVSDRNGGPQVFVVPATGGTPRQVSF
                     NGSYNTTPTWMPKAGKHVIAYTTRDGNTYDIVTLDLDTKAMIRITQNEGSNEEPSFSP
                     NGRAIAFARAGQGVFIASADGTGKAVKVWSGSATGVDWGPAPAP"
     gene            complement(3535..4251)
                     /locus_tag="VF516_45020"
     CDS             complement(3535..4251)
                     /locus_tag="VF516_45020"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEX8114996.1"
                     /translation="MSFNNGQMHGLALVGTVVLSGALFGGTLYLTEAAEATHPPELKE
                     MITIEASLARTSEKKKQPQKELKAPEPEVKPEGVSRDEKKPPPTKKPEDKKPKKPDDK
                     PPDIHQFHHASDDDPQPGAAPVTDIGQFDGSEKGFAPINAGNPYWQNLAADNHQAWQI
                     PTISKVNGTPVGCFHIAPDGKIAAVKFDAPSGDPILDDSVQRALKTVQKLRNDKPQPV
                     PTEQLGVIKRWVCFRFDPNT"
     gene            complement(4295..4744)
                     /locus_tag="VF516_45025"
     CDS             complement(4295..4744)
                     /locus_tag="VF516_45025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEX8114997.1"
                     /translation="MAMKAGSGDELNAEINVTPFVDVMLVLLIIFMITAPMMNNTGVE
                     LQLPQVAAKNIDDPEGKLVLSIKKTSEIFLGGTQVTWQDLAAKLKANERVKREAALYI
                     EADTNLPYGVVLTAMAVAKDSGVEKVMMLTDPSDQLKLSDLDQKAKH"
     gene            complement(4769..5401)
                     /locus_tag="VF516_45030"
     CDS             complement(4769..5401)
                     /locus_tag="VF516_45030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEX8114998.1"
                     /translation="MVLLALMFVVGLYIIIYKTLYVRRATSESTRFTDSFWRSRDIEQ
                     IYKQAQALRNSPISQMFVAGYTELAKLSSDEHLRGDREGNLANIERALRRAQVVETTK
                     LEAMVPFLATTGSAAPFIGLFGTVMGILFAFLDIAPDPSGPARPAAASLTTVGPHIAE
                     ALFATAIGLVAAIPAVMAYNYFTRRIRVLRSEMDTFEQDYLNIIKRHFLR"
     gene            5586..6104
                     /locus_tag="VF516_45035"
     CDS             5586..6104
                     /locus_tag="VF516_45035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8114999.1"
                     /translation="MCLAVHACDLGNGGAVELSWSLRPASGEGSNKFVGCERSEQDSN
                     GTQVQVRAITRVRLHWTVEGAACSRAWSCDDNHGATRFEVPRGTADLTVTLECGAPTP
                     GLSECEDDHPADPATYTTPAIVQRDSIRGEVVTLGAVELVVSDAACPQEPCTCPIALP
                     TSAQVATDHTSR"
     gene            6326..>6962
                     /locus_tag="VF516_45040"
     CDS             6326..>6962
                     /locus_tag="VF516_45040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115000.1"
                     /translation="MKNLVLGALLTAAVSSAACGTTSDTSARITASWSFNTYANRASA
                     PTAPCPTGFDTATVYVRPWDPFVGDFTGPALASDLFDCSAKVGTTGLLDGVFQVWVQI
                     ENHDGSSVYARSEAEVIDTADGDQSISLPTLFTDAGYFDLSWDLLRAGSTQRLRCADA
                     GITSAGRISTSAQMTSGGTIYVDKFSCDDGYGITEELPVGLYDVTVTVTASP"
BASE COUNT         1034 a         2358 c         2404 g         1166 t
ORIGIN      
        1 gccggcctcc tgggccgagc gcgtgatgtt gccgtcgttg cgcgccatca gcgccgacag
       61 gtacgcgatc tcgaacgcgt cgaccacggt ctgcttggcg cccttgaacc ccaggccggc
      121 cttgagcagc gccgggtcga acgtcgccgc gccggtctgg ggcggcagct cgacgcccga
      181 catgctggcg gcggcgcgct gcgcggcctg ggcgccggcc tgggccagat catgcgacac
      241 cacgacgctg gggcccatct cgcggccgaa caccaggtca gtgcgcgtga tcgtcgggcc
      301 gtcgctcagc gccgcggcgc gctcgaccac gttgcgcatc tcgcggacgt tgcccggcca
      361 gctgtgggtg accagcgcgg ccagcgcgtc ctgcgaccat gccatcgcca tgccgcggcg
      421 cgcgccgatc tcgcgcagga agtgggtcgc cagccccggg atgtcctccc tgcgctcgcg
      481 cagcggcggc agctcgacgt ggacgaccga cagccggaag tataggtcct cgcggaacgt
      541 gcccttgttg acctcctcgc ggaggtcgcg gttggtcgcg gccagcacgc ggacgtcgac
      601 cttgatcgtg cggtcgccgc cgacccgctt gatctcgcgc tgctcgagca cgcgcaacag
      661 cttgggctgc agcatgatgt cgagctcgcc gatctcgtcg aggaagatcg tgccgccgct
      721 ggcctgctcg aagcagccga cgtgctgggc caccgcgccg gtgaagctgc ccttctcgtg
      781 gccgaacagc gtcgactcga tcagctcgcg cgggatcgac ccgcagtcga gcacgacgaa
      841 cggccggctc ttgcggcccg acgcgttgtg cagcgcgcgc gcgaccatct ccttgccggt
      901 gccggtctcg ccggtgatca ggcaggtcag gtcgctcggc gcgaccttct ccaggtgggc
      961 gaagatctcg cgcatcgcgg gcgacgcgcc gagcatcgcg tcgaaccggt ccttcttgga
     1021 cagctcgatc tcgaccacgt cctccagggt ctggaactgg atgttggtgt ggccgatccg
     1081 gaacaccgtg cccggccgga ggaacacctc gcggatccgg agatcgccga cgaagatccc
     1141 gttgcgcgag ttgaggtcgc gcagccggta gccgtcgtcg cgcgccgaca cctcgaagtg
     1201 ggtgcccgac accgccttgt cctggaccac caggtcgctg atgatgctgc ggccgccggt
     1261 gacccgcggc ttggcgatct ccaccgactt accctgatcc gcgccggaca cgaccacgag
     1321 cttgcccttg cgcagccggc ggaccgtcgc ccagtcgccc tcgaacagcg tggtcaggcc
     1381 ggtccccgtc tcgttgggat cgaaggggcc ggcggatccg gggacgtctg ccatcgcgct
     1441 caacctacca aatcgcagcc cggcccggcg ctaattcagc cacttgtgct cgtcgcgcgg
     1501 cggggacgag cgcgccttgc cgcccgacat cacggtcaac cgtgcgcggg cgcgcgcgat
     1561 ccgccagcgc ttccaggcca gcgccgggct ccaccgcttc gaggtcagga tcgccgccgc
     1621 gagcatggca gccgcgtagg ccacaccgtc ggcccagcgc tgcgacagca cgatcaacag
     1681 cgcgaggaac gcgaggatgc cgtacatcag ctggcggccg gtcaacggca ggacgccgaa
     1741 gaactgcacc ggctggcggg cgtagacgat gccgaacgca acgatcgccg cgtagatgaa
     1801 cggatcgagc cccccgaaca tcgcgttcag atcgccgagc gcgtaggcaa acccgcagcc
     1861 ggctgcggtg gcgaccagcg acgtgatcgc gacgaaccgg tagaaccgcg cggtccccca
     1921 gaaccgctcc agcgtcggca cgaacatcca cagcacgagg ccgtggagca gcagggcgag
     1981 cagcttgggc tcgaggaaca cgccggtggc cagcgtccat acccggccgt gcacgaacac
     2041 gttggccggg gtggccatca ggtagtcgct gaaccacttc tgggtcgcag gatccagcgc
     2101 cagccagacc agcgatagcc cgacctccag gcccagcacg atcaaggccc cgcgggtgat
     2161 ccgcatgccg ctgaaccggg agcgctgcgc gccgctcacg cgcacaccat agcacctcgc
     2221 ccgccgcgcg tcaggccata ccgacggggg gtcagggcgc cggcgccggg ccccagtcga
     2281 cgccggtggc cgagccgctc cacaccttga ccgccttgcc ggtgccgtcg gcgctggcga
     2341 tgaacacgcc ctggccggcg cgggcgaacg cgatcgcgcg cccgttgggc gagaacgacg
     2401 gctcctcgtt ggagccctcg ttctgggtga tccggatcat cgccttggtg tcgaggtcga
     2461 gcgtgacgat gtcgtacgtg ttgccgtcgc gcgtggtgta cgcgatgacg tgcttgccgg
     2521 ccttgggcat ccaggtcggc gtggtgttgt agctgccgtt gaacgatacc tggcgcggcg
     2581 tgccgccggt cgccgggacg acgaagacct gcgggccgcc gttgcggtcg ctgacgaacg
     2641 cgagctggct gccgtcgggc gaccacgcgg gcgaggtgtc gatcgcgcgg tcgttggtga
     2701 tccggcggac gatgctgccg tcggcggcgc tgatcacgta gatctcgggg ttgccgtcct
     2761 tggacagggt gatcgcgagc tgcttgccgt cgggcgacca cgcggcgccg gtgttcatgc
     2821 cgcgctggct cgacagcttg ttcggccggc cgccgccggc gggcgcgacg tacagatccg
     2881 ggttgttgcg catgaacgac gtgtaggcga tctgcccgcc gctcggcgcc cacgccggca
     2941 ggatgttctg cgagctgttg ttcgagaccg cgtacgcgct ggcgccgtcg aagtccatcg
     3001 cgtagatcgc gctcgcggtc ttgcccttgg cggtgaacgc gatcttcgag ccgaagaacc
     3061 cgggctcgcc ggtgtagtac ttgacgacct cgttgcagaa ccgatggacg atcgtccgcg
     3121 tgtcgccctt gcgcgggtag accttgctca gcaccgaggt cgtgcccttg gagatctcgt
     3181 agagccggag ctcgatctcc gacgcggtcg cgcggtactt gatcacgccg aacgcgccga
     3241 cgtccttcca cttctgcgga tcgatgatca gcccctcggc cttgagatcg gcgaggaacc
     3301 cggccggatc gaggaccttg aacacgccgg cgaccgacag gtcgaacgac gcgacctgcg
     3361 cgatctcgcg cgcggccgcc gcgtcgccct cctgcgacgc cggcacggcg atcgggtaca
     3421 gcgtgcggtt ggcgcccttg acgtcgatca ccaccccgcc gctggggtca tcggcccgcg
     3481 cgggcccccc gaggctgacg aggcttgtga tcgccgcggc gagcgccgcg aggactacgt
     3541 gttggggtcg aatcgaaaac atacccatct cttgatgacg ccgagctgct cggtgggaac
     3601 tggctggggc ttgtcgttgc ggagcttctg gacggtcttg agcgcgcgct gcaccgagtc
     3661 gtcgaggatc gggtcgccgc tcggggcatc gaacttgacg gccgcgatct tgccatcggg
     3721 cgcgatgtga aaacacccca ccggtgtgcc gttcaccttc gaaatcgtcg ggatctgcca
     3781 ggcctggtgg ttgtcggccg ccaggttttg ccagtacggg ttgcccgcgt tgatcggcgc
     3841 gaagcccttc tcgctgccgt cgaactggcc gatgtcggtc accggcgccg cgccgggctg
     3901 gggatcgtcg tcgctggcgt ggtggaactg gtggatgtcg ggcggcttgt cgtccggctt
     3961 cttgggcttc ttgtcctcgg gcttcttcgt gggcggcggc ttcttctcgt cgcgcgagac
     4021 gccctcgggc ttgacctcgg gctcgggcgc cttgagctcc ttctgaggct gcttcttctt
     4081 ctccgacgtg cgggcgagcg acgcctcgat cgtgatcatc tccttgagct cgggcgggtg
     4141 cgtcgcctcg gcagcctcgg tcaggtacag cgtcccgccg aacagcgcgc cggacagcac
     4201 gaccgtcccg acgagggcga gtccgtgcat ctggccgttg ttgaagctca tgctcataga
     4261 gacgttcggc gcgggcgttt gttcgccgcc ctcgctaatg cttcgctttc tggtcgagat
     4321 cggacagctt gagctgatcg ctggggtcgg taagcatcat taccttctcg actccggaat
     4381 ccttggccac ggccatcgcg gtgaggacga ccccgtacgg caggttggtg tcggcttcga
     4441 tgtacagggc agcctctcgc ttgacgcgct cgttggcctt gagcttggcg gcgagatctt
     4501 gccacgtgac ctgggttccg ccgagaaaga tctcgctggt cttcttgatc gacagcacca
     4561 gcttgccttc ggggtcgtcg atgttcttgg ccgcgacctg cggcagctgg agctcgacgc
     4621 cggtgttgtt catcatcggc gcggtgatca tgaagatgat cagcagcacg agcatgacgt
     4681 cgacgaacgg cgtcacgttg atctcggcgt tgagctcgtc gccgctgcct gctttcatcg
     4741 ccatggcggg tcgtcctggt ccgcgcgctc agcgcaagaa gtggcgcttg atgatgttga
     4801 ggtagtcctg ctcgaaggtg tccatctccg agcgcagcac ccggatccgg cgcgtgaagt
     4861 agttgtacgc catcacggcg gggatcgcgg cgaccaggcc gatcgcggtc gcgaacagcg
     4921 cctcggcgat gtgcgggccg acggtggtga gcgacgcggc ggcgggccga gccgggccgc
     4981 tcggatcggg cgcgatgtcg aggaacgcga acaggatgcc catcacggtg ccgaacaggc
     5041 cgatgaacgg cgccgccgag ccggtggtcg cgaggaacgg caccatcgcc tcgagcttgg
     5101 tggtctcgac gacctgggcg cggcgcagcg cgcgctcgat gttcgcgagg ttgccctcgc
     5161 ggtcgccgcg caggtgctcg tcggaggaga gcttggcgag ctcggtgtag ccggcgacga
     5221 acatctgcga gatcgggctg tttcgcagcg cctgggcctg cttgtagatc tgctcgatgt
     5281 cgcgcgatcg ccagaacgag tcggtgaacc gggtcgactc gctggtcgcg cgccgcacgt
     5341 acagcgtctt gtagatgatg atgtagagcc cgacgacgaa catcagggcg aggaggacca
     5401 tgacggcctt gacgatccag tgcgcctggt cgaacaggcg cgggatgtcc aggtttgccg
     5461 caagcgtgga tgccggtgaa aaccctggca taatgagcca cggcgatagc atgcaccccg
     5521 tctccgggtc aaatcgcgcg gccaggccgg cccgtagcct gcggatacgt atcgcgatcg
     5581 cgacattgtg tctggccgtt cacgcctgcg acctgggcaa cggcggtgcg gtcgagctgt
     5641 cgtggagcct gcgacctgcg tccggcgagg ggagcaacaa gttcgtcggc tgcgagcgca
     5701 gcgaacagga ctcgaacggc acgcaggtcc aggtccgagc gatcacgcgg gtccggctgc
     5761 actggacggt cgagggcgcg gcgtgctcgc gcgcctggag ctgcgacgac aaccacggcg
     5821 ccacccgctt cgaggttcct cgaggcaccg cggacctcac ggtgacgctg gagtgcggcg
     5881 ccccgactcc cgggctctcc gagtgcgagg acgatcaccc cgctgacccc gccacgtaca
     5941 ccactcccgc gatcgtgcag cgcgactcga tccgcggcga agtcgtgacc ctgggtgcgg
     6001 tcgagctcgt ggtgtcggac gcggcctgcc cgcaggaacc atgtacgtgc ccgatcgccc
     6061 ttccgaccag cgctcaggtt gcgacggatc acacgagccg gtaaccggct ggcgcagcgc
     6121 ttgcctgtgg aagcgtggtg aagcaaatcg ggggagcacc gtccaatccg atcacagcgc
     6181 cagccatcgc tctcgtgtcg acgaagaccg gcggctgtac gacctgcacg cctactcgat
     6241 caccttctgc acccgccgcg attccgatcg acggctgatg acttcgcact gcctggcttt
     6301 ggtactttca tctcaggaga tccgaatgaa gaacctcgtc ttgggagcac tgctcactgc
     6361 agccgtctcg tccgccgcct gcggcacgac ctccgacacc agcgcccgga tcaccgcgag
     6421 ctggtcgttc aacacgtacg ccaaccgcgc tagcgcgccg accgcgccgt gtccgaccgg
     6481 cttcgacacc gcgacggtgt acgtccggcc atgggatccg ttcgtcggcg acttcacggg
     6541 cccggccctc gcgtcggacc tgttcgactg ctcggccaag gtcgggacga ccgggctgct
     6601 cgacggcgtg ttccaggtct gggtccagat cgagaaccac gacggctcct cggtctacgc
     6661 gcgctccgag gcggaggtca tcgacaccgc ggacggcgat cagtcgatca gcctgccgac
     6721 cctgttcacc gacgcggggt acttcgacct gagctgggac ctcttgcggg cgggctcgac
     6781 ccagcggctg cgctgcgcgg acgccgggat cacctcggcc ggccggatct cgacctcggc
     6841 gcagatgacg agcggcggca cgatctacgt cgacaagttc tcgtgcgacg acggctacgg
     6901 gatcaccgag gagctgccgg tcgggctcta cgacgtgacg gtgaccgtca ccgcctcgcc
     6961 cg
//
LOCUS       DASVOR010002457         5023 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_406047,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002457 DASVOR010000000
VERSION     DASVOR010002457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5023
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406047"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..683)
                     /locus_tag="VF516_45045"
     CDS             complement(<1..683)
                     /locus_tag="VF516_45045"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8115001.1"
                     /translation="MKVVFLAPSYPPEMQQYTRGLAEVGAEVYGVGDAPAQALPAGLK
                     RYLHDYLQVPRLLAEADVVDRVHAWLRGREVDRVLANWEVMVLTAARLRERFGVPGMT
                     VDTVNGFRDKQLMKERVAAAGLRVPRSARVRTAAEVHAAAEQIDFPLIVKPIAGAGSA
                     DTYRVDDAAALDSVLARMQHVREASCEEYIDGEEFTADTVSIGGKPAYLNVAQYLPKP
                     IVARSVEWI"
     gene            708..1922
                     /locus_tag="VF516_45050"
     CDS             708..1922
                     /locus_tag="VF516_45050"
                     /inference="COORDINATES: protein motif:HMM:NF014298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEX8115002.1"
                     /translation="MRNVVFVAPFPLDTTMRFARAAAGLDDVRMFGVLQEPPRGTDAG
                     MFADLVTVNDGLDTSQLIAAARVLVRRHGAIHRVLGILEALQVQLGEMRRALAVPGPG
                     PDTAELFRDKARMKDELGRHGLPCARHRLLRSWNDAEAMIAEIGLPVVLKPPAGMGCK
                     ATWRVRTVDELRGALAAIHASPDNPALAEELLVGREHSFETITINGQVRFHSLSRYYP
                     TPLEVMETPWIQWVCVLPRDVSGPEWSDAVELGVRAVTALGLDTGFTHMEWFRRDDGS
                     LAIGEIAARPPGAHIVRMTGLAHDADMYRAWARAVVDDAYDGPYARRYAVGCAYLRGV
                     GRGRVLRVTGVDRAQELVGRHVVEARLPEPGAPRSDSYEGDGYVIVRDPDTEVVTAAM
                     KTIIETIQVQYG"
     gene            2046..2678
                     /locus_tag="VF516_45055"
     CDS             2046..2678
                     /locus_tag="VF516_45055"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115003.1"
                     /translation="MHTQLSRFLFTAILSGAILGSAGRSYADDHRGDRREERREDRRE
                     ERREEHRGDVRGDVRVEVRDHGRPYQAPPAVRYERHENRRGYVWVNGGYDWRDGRYVW
                     VGGHHERERPGFRWREPRWEMRDGAYVRIDGGWVGLGPVVAPPVVREERWETRPGHIW
                     VRGHWDWVNGSWTWIPGHHERERAGYVWREPRWEMRDGVYVKVEGTWVVR"
     gene            complement(2679..3206)
                     /locus_tag="VF516_45060"
     CDS             complement(2679..3206)
                     /locus_tag="VF516_45060"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEX8115004.1"
                     /translation="MGTVRTSNQGITTDVLELLSAQHREVDDLLEKLEQGNGDREALF
                     VELADKLAAHAAAEEKVFYPSVMAEQTSALLHESVEEHLAIKRVLADLIAMKVDDDEF
                     QAKLAVLKEEVSHHAHEEEEGKLFPELRAALSRDELAALGNEFLVVFETLMQSHPYRN
                     VPGETKEAAPLPPAS"
     gene            complement(3385..4281)
                     /locus_tag="VF516_45065"
     CDS             complement(3385..4281)
                     /locus_tag="VF516_45065"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HEX8115005.1"
                     /translation="MRSEVVSLTSVLERAVEIAAPLITRHRHVLHAQGSEGIFLQGDP
                     VRLAQIFGNLLVNAAKFTPPGGKLDVEVERHVDRVRVTVRDTGRGIARDQLVRIFDPF
                     VQANRERDALSGGLGLGLTIVENLVVRHGGSIAAHSEGPGCGAAFTVELPTVAHPVES
                     AAVAWPQARGARAGIRVLVVDDNTDVAELLSEALQIEGFQTAVEHDAMAALERWRSFA
                     PHAAVLDVGLPELDGYELARKLRAEHGTVPTLIAATGYGQRKDRMRAADAGFDCHFVK
                     PVSVRDLVKVLDQRIVNAAAPA"
     gene            complement(4215..4565)
                     /locus_tag="VF516_45070"
     CDS             complement(4215..4565)
                     /locus_tag="VF516_45070"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HEX8115006.1"
                     /translation="MSLNVCLSEAQFRDHFHVCAFVRGPSEERELTDPFFVEGLRLGE
                     KAVYYVDPAQRDEHEARLRTSAPSPDLVEVTTWHDAHLKGGSFDPDRMMASCAARWSR
                     SRRSSSARSRSRRR"
     gene            complement(4820..>5023)
                     /locus_tag="VF516_45075"
     CDS             complement(4820..>5023)
                     /locus_tag="VF516_45075"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HEX8115007.1"
                     /translation="AHLDAMTRIAEGDPDAGPVARLEQSERFHWLVAPSSTVIQTSPV
                     HTGLCDDPGAALDQLIERLVAVR"
BASE COUNT          727 a         1794 c         1780 g          722 t
ORIGIN      
        1 ctgatccact cgaccgagcg cgccacgatc ggcttgggca ggtactgcgc gacgttcaga
       61 taggccggct tgccgccgat cgacacggtg tcggcggtga actcctcgcc gtcgatgtac
      121 tcctcgcagc tggcctcgcg cacgtgctgc atgcgggcga gcacgctgtc gagcgcggcg
      181 gcgtcgtcga cgcggtaggt atcggcgctg cccgcgccgg cgatcggctt gacgatcagc
      241 ggaaagtcga tctgctcggc ggcggcatgg acctcggccg cggtgcgcac gcgtgccgag
      301 cgcgggacgc gcaggccggc ggcggcgacg cgctccttca tgagctgctt gtcgcgaaag
      361 ccgttgacgg tgtcgacggt catgcccggc acaccgaacc gctcgcgcag gcgcgccgcg
      421 gtcagcacca tgacctccca gttggcgagc acgcgatcga cctcgcggcc gcgcagccag
      481 gcgtggaccc ggtcgacgac gtcggcctcg gcgagcagcc gcgggacctg gaggtagtcg
      541 tggaggtagc gcttgaggcc ggccggcaag gcctgggccg gcgcgtcgcc gacgccgtag
      601 acctcggcgc cgacctcggc cagcccgcgg gtgtactgct gcatctccgg cgggtagctc
      661 ggcgcgagga acacgacctt catcccgggc atgttacaac gcacgtcatg cgcaacgtgg
      721 tgttcgtcgc gccgttcccc ctcgacacga cgatgcggtt cgcgcgcgcc gccgccgggc
      781 tcgacgacgt ccgcatgttc ggcgtcctgc aggagccgcc gcgcggcacc gacgccggga
      841 tgttcgccga cctggtgacg gtcaacgacg ggctcgacac ctcgcagctg atcgcggcgg
      901 cccgcgtgct ggtgcgccgc cacggcgcga tccaccgcgt gctcggcatc ctcgaggcgc
      961 tccaggtcca gctcggcgag atgcgccgcg cgctcgccgt gcccggcccc ggccccgaca
     1021 ccgccgagct gttccgcgac aaggcgcgga tgaaggacga gctcgggcgc cacggcctgc
     1081 cgtgcgcgcg ccaccgcctg ctgcggtcgt ggaacgacgc cgaggcgatg atcgccgaga
     1141 tcggcctgcc ggtggtgctc aagccacccg ccggcatggg ctgcaaggcg acctggcggg
     1201 tccgcaccgt cgacgagctg cgcggcgcgc tcgccgcgat ccacgcctcg cccgacaacc
     1261 cggcgctcgc cgaggagctg ctggtcggcc gcgagcacag cttcgagacc atcacgatca
     1321 acggccaggt ccggttccac tcgctgtcgc gctactaccc gacgccgctc gaggtgatgg
     1381 agacgccgtg gatccagtgg gtgtgcgtcc tgccgcgcga cgtgtccggc cccgagtggt
     1441 ccgacgcggt cgagctcggt gtccgcgcgg tcaccgcgct cgggctcgac accgggttca
     1501 cccacatgga gtggttccgg cgcgacgacg gatcgctggc gatcggcgag atcgcggccc
     1561 ggccgcccgg cgcccacatc gtgcggatga ccggcctcgc gcacgacgcc gacatgtacc
     1621 gggcgtgggc ccgcgcggtg gtcgacgatg cgtacgacgg gccgtacgcc cggcgctacg
     1681 cggtcggctg cgcctacctg cgcggcgtcg gccgcggccg cgtgctgcgg gtcaccggcg
     1741 tcgatcgcgc gcaggagctg gtcggccgcc acgtggtcga ggcccggctg cccgagcccg
     1801 gcgcgccgcg cagcgacagc tacgagggcg acggctacgt gatcgtccgc gatccggaca
     1861 ccgaggtcgt caccgccgcg atgaagacga tcatcgagac gatccaggtg cagtacggat
     1921 aggtccgccg ccgccgatgt gggttcactg ggacctcaca ccgacacccg cggcgcgaaa
     1981 atgaaccttt cgtgaatgcc gcgagtcgaa gcgtcgtaca ctcgatccgt ccgtcggagg
     2041 taggcatgca cacacaactg tctcggttcc tgttcaccgc gattctaagt ggggcaatcc
     2101 tcggttcggc ggggcgctcg tacgccgacg atcaccgcgg cgaccgccgc gaagagcgcc
     2161 gcgaggaccg gcgcgaagaa cgccgcgagg agcaccgcgg cgacgtccgc ggcgacgtcc
     2221 gcgtggaggt ccgcgaccac ggccgcccgt accaggcgcc gccggcggtc cgctacgagc
     2281 gccacgagaa ccgccgcggc tacgtctggg tcaacggcgg ctacgactgg cgcgacggcc
     2341 ggtacgtctg ggtcggcggc caccacgagc gcgagcggcc cggcttccgc tggcgcgagc
     2401 cgcgctggga gatgcgcgac ggcgcgtacg tccggatcga cggcggctgg gtcgggctcg
     2461 gcccggtcgt tgccccgccg gtggtgcgcg aggagcgctg ggagacccgg cccggccaca
     2521 tctgggtccg cggccactgg gactgggtca acggcagctg gacgtggatc ccggggcacc
     2581 acgagcgcga gcgcgccggc tacgtctggc gcgagccgcg ctgggagatg cgcgacggcg
     2641 tgtacgtcaa ggtcgagggc acctgggtcg tccgctgatc acgacgcggg cggcagcggc
     2701 gccgcttcct tggtctcgcc cggcacgttc cggtacgggt gcgactgcat cagggtctcg
     2761 aacacgacca ggaactcgtt gcccagcgcg gccagctcgt cgcgcgacag cgccgcgcga
     2821 agctcgggga acagcttgcc ctcctcttcc tcgtgcgcgt ggtgcgagac ctcttccttg
     2881 agcacggcga gcttggcctg gaactcgtcg tcgtcgacct tcatcgcgat caggtcggcg
     2941 agcacgcgct tgatcgcgag gtgctcctcg acggactcgt ggagcagcgc gctggtctgc
     3001 tcggccatca ccgaggggta gaacaccttc tcctcggccg ccgcgtgcgc ggccagcttg
     3061 tcggcgagct ccacgaacag cgcctcgcgg tcgccgttgc cttgctcgag cttctcgagc
     3121 aggtcatcga cctcgcgatg ctgggcggag agcagctcga gcacgtcggt ggtgatgccc
     3181 tggttggacg ttctgacggt tcccatgctg tcccgcaagt gcaagcacga ggccatggcg
     3241 cgcgactcgc tccacgccac gcgggtcgca ccgactgctg tccgcccacc gacgatcgcg
     3301 gcgtacgacg cagttccgcc gagcgcgaca gcacgtgtgg cacgggtgcc cgtgcatcgt
     3361 tcgatgctcc ccgggccgtc tcggtcacgc cggcgcggcg gcattcacga tgcgctgatc
     3421 cagcaccttc acgaggtccc ggacgctcac gggcttgacg aagtggcagt cgaaaccggc
     3481 gtcggcggcg cgcattcgat ccttgcgctg accgtagccg gtggccgcga tcagcgtcgg
     3541 gacggtgccg tgttccgcgc gcagcttcct cgcgagctcg tagccgtcga gctcggggag
     3601 cccgacatcg agcaccgcgg catgcggcgc gaagcttcgc cagcgctcca gcgcggccat
     3661 ggcatcgtgc tcgacggcgg tctggaaacc ctcgatctgc agcgcctcgg acagcagctc
     3721 cgcgacgtcg gtgttgtcat cgacgaccag cacgcgaatc ccggcgcgcg cgccccgggc
     3781 ctgtggccac gcgaccgcgg cggattccac cggatgggcc accgtgggga gctcgacggt
     3841 gaaggccgct ccacaccccg gaccctcgct gtgtgccgcg atcgagccgc cgtgccggac
     3901 cacgagattc tcgacgatcg tgaggccgag accgaggcca ccgctcagcg catcacgctc
     3961 gcggttggcc tgcacgaacg gatcgaagat ccgcacgagc tgatcgcgcg cgatcccgcg
     4021 ccccgtgtcg cgcaccgtca cgcggacgcg atcgacgtgg cgctcgacct ccacgtcgag
     4081 cttcccgccg ggcggtgtga acttggctgc gttcaccagc aggttgccga agatctgggc
     4141 gagccgcacc ggatcgccct ggaggaagat gccctcggag ccctgcgcgt ggagcacgtg
     4201 gcggtggcgt gtgatcaacg gcgccgcgat ctcgaccgcg cgctcgagga ccgacgtgag
     4261 cgagaccacc tcgctgcgca actcgccatc atccggtcgg ggtcgaacga cccgcccttg
     4321 aggtgggcgt cgtgccaggt cgtcacctcg accaggtcgg gcgacggcgc ggacgtccgg
     4381 agccgcgcct cgtgctcgtc gcgctgcgcc gggtcgacgt agtacacggc cttctcgccg
     4441 aggcgcagtc cctcgacaaa gaacgggtcg gtgagctcgc gctcctcgga gggccccctg
     4501 acgaaggcgc agacatgaaa gtgatcgcga aactgcgctt ccgaaagaca gacattcagc
     4561 gacacggcgg cagcctagcc tagcacacgg gcagtgaccc agaccgccgt tccggcgcat
     4621 tgcgcgcgct gcgcggatcg ccgatgccgc cgctcgcatg agtctccagg agggagggga
     4681 aatccgctca tgcgagaacc catgtacacc ggagcgcacc gtgcgctggc ggcacagctg
     4741 gggaatctcg atcgcgattc tctactgacc atctggatcg gcttcgaccg ggagtcggtg
     4801 ttgcgccgac gaaacattcc taccggaccg cgaccagccg ctcgatcagc tggtcgagcg
     4861 ccgcgccggg gtcgtcgcac agcccggtgt gcaccggcga ggtctggatc accgtgctcg
     4921 acggcgccac cagccagtgg aagcgctcgg actgctcgag ccgcgcgacc ggcccggcat
     4981 ccggatctcc ctcggcgatc cgcgtcatcg catcgaggtg cgc
//
LOCUS       DASVOR010002458         2366 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_231725,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002458 DASVOR010000000
VERSION     DASVOR010002458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231725"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            622..>2366
                     /locus_tag="VF516_45080"
     CDS             622..>2366
                     /locus_tag="VF516_45080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8115008.1"
                     /translation="MTDTERVLANGAEAGALARSIDWSKTAIGPVERWSQALRSTVAL
                     VLHNHSPMLLWWGPEFIQIYNDAYRPVPGDKHPRALGQPASECWSEIWHIIGPMVEAP
                     FRGGPASVSDDLLLPIRRKSFVEETHFQVAYSPVPDETVPGTGIGGVLATVTETTEQM
                     YAERQLRTLRELGARGAAEVKTVEAACTAAAATLADNRWDVPFAVFYLLDDAGARARR
                     IANVGFDDAKLALAAPAEVDLAADPCPWPLRQVAADRQIALVSDLAHCGDALPRSPWA
                     ELPREAIALPLASPDQATAYGVLICGVSPHRTLDAGYRTFLELAAAQVVAAIRNARAL
                     EQERKRAEALAEIDRAKTAFFSNVSHEFRTPLTLMLGPTEDALAMADPALRGADLETV
                     YRNELRLLKLVNALLDFSRIEAGRMRASYAPTDIAARTAELASGFRSAVERAGLAYRV
                     DCAPIDEPIYLDVDMWEKIVLNLLSNALKFTFAGGIEVGLRRRGAEVALTMKDTGVGI
                     PAGELPRLFERFHRVAGARARTYEGSGIGLALVTDLVRLHGGRVEVASELGAGTTFTV
                     ILPTGRHHLDPEQIAP"
BASE COUNT          342 a          859 c          843 g          322 t
ORIGIN      
        1 tgccagccga tgacagcgcg atgacagcag atttccgagc accgtgatcg gctgtggatt
       61 tcacggcgca tgccaccatg gcggacagca atcctcatcg gttgggtccg ccgacccggg
      121 gccggtcgcc tcggcgagcg gcagtcgccc ctgtgggatt cctgcacgtg cggccgctgg
      181 gcgagtggct gatcggcgtg gagcacgagt cgcaggacgc ttccccagat ccagcgccat
      241 acgctgaccg ccacgccgac cgggtcgcgg cgggcccggc cgccgggcag cagatccgcg
      301 atgaccgaca cgaccctgaa tcgcccgttc agcgccatgc ggggtatgcg gggcggtggc
      361 gcgtgacatg cccgcacggc gggctctcgc gcacggcagc ggagttccga ggcagtccgg
      421 attccgtggc cacgacgcga gcgctccaga gctcgcagtg gcgaagaatg cgtggaacta
      481 cgggctgaca cggatgcgcg gtccacagcg cacagcgccc cacgaggcga ggcgcacagt
      541 ctggcgcgct gactgcatag cacgtcgccg gctgtgcccg atcgcgcaga tcaccacagc
      601 gttcaactcg caatcatcgt catgacggac acggaacgcg tgctcgccaa tggcgcggag
      661 gccggcgcac tggcccgctc gatcgactgg tcgaagacgg cgatcgggcc ggtcgagcgc
      721 tggtcgcagg cgctgcgctc gacggtcgcg ctggtcctgc ataaccactc gcccatgttg
      781 ctgtggtggg ggccggagtt catccagatc tacaatgatg cctaccggcc ggtgcccggt
      841 gacaagcacc cgcgcgcgct cggccagccg gccagcgagt gctggtccga gatctggcac
      901 atcatcggcc cgatggtcga ggcgccgttt cgcggcgggc cggcgtcggt cagcgacgac
      961 ctcctgctgc cgatccggcg caagagcttc gtcgaggaga cccacttcca ggtcgcctac
     1021 agcccggtcc ccgacgagac cgtcccgggg accgggatcg gcggcgtgct cgccacggtc
     1081 accgagacca ccgagcagat gtacgccgag cgccagctcc gcacgctgcg cgagctcggc
     1141 gcccgcggcg cggccgaggt caagacggtg gaggcagcgt gcacggccgc cgcggcgacg
     1201 ctggcggaca accggtggga cgtgccgttc gcggtgttct acctgctcga cgacgccggc
     1261 gcgcgcgccc gccggatcgc gaacgtcggg ttcgacgacg cgaagctcgc gctggccgcg
     1321 cccgccgagg tcgacctcgc ggcggacccg tgcccgtggc cgctccgcca ggtcgcggcg
     1381 gaccggcaga tcgcgctggt gtccgatctc gcgcactgcg gtgatgcgct gccgcgcagc
     1441 ccgtgggccg agctgccgcg cgaggcgatc gcgctgccgc tcgcatcgcc cgaccaggcg
     1501 accgcgtacg gcgtgctgat ctgcggggtc agcccgcacc ggacgctcga cgcgggctat
     1561 cgcacgttcc tcgagctcgc cgcggcgcag gtcgtcgccg cgatccgcaa cgcgcgcgcg
     1621 ctggagcagg agcgcaagcg agccgaggcg ctcgccgaga tcgaccgcgc caagaccgcg
     1681 ttcttctcca acgtcagcca cgagttccgg accccgctga cgctgatgct cgggccgacc
     1741 gaggacgcgc tggcgatggc cgaccctgcc ctgcgcggcg cggatctcga gaccgtctat
     1801 cgcaacgagc tgcggctgct caagctggtc aatgcgctgc tcgacttctc gcggatcgag
     1861 gccggccgga tgcgcgcgag ctacgcgccg accgacatcg cggcgcgcac cgcggagctc
     1921 gccagcgggt tccgctccgc ggtcgagcgc gcgggcctcg cgtaccgcgt cgactgcgcg
     1981 ccgatcgacg agccgatcta cctcgacgtc gacatgtggg agaagatcgt cctcaacctg
     2041 ctgtcgaacg cgctgaagtt cacgttcgcc ggcgggatcg aggtcgggct gcgccgccgc
     2101 ggcgccgagg tcgcgctcac catgaaggac accggggtcg ggatcccggc cggtgagctg
     2161 ccccggctgt tcgagcgctt ccaccgggtc gccggtgcca gggcccggac ctacgagggc
     2221 tccggcatcg gcctggcgct ggtcaccgac ctggtccggc tgcacggtgg ccgcgtcgag
     2281 gtcgcgagcg agctcggcgc cgggaccacg ttcaccgtga tcctcccgac cggccgccac
     2341 cacctcgacc ccgagcagat cgcgcc
//
LOCUS       DASVOR010002459         1587 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_231759,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002459 DASVOR010000000
VERSION     DASVOR010002459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231759"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1587
                     /locus_tag="VF516_45085"
     CDS             <1..>1587
                     /locus_tag="VF516_45085"
                     /inference="COORDINATES: protein motif:HMM:NF021942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HEX8115009.1"
                     /translation="NLEIRDVRLVYAPARSVGDFGGEVDNWHWPRHTGDWSFYRAYVG
                     KDGNPADYSPDNVPFHPKAHLQVTTAGLKPGDFVMVTGYPGRTERTRTAADIHHEVEW
                     ALPYQIAYLEERYKIAEAHLTDDGETRTRATVMKQGTQNGLEKDRGVLAGLTRGDLLA
                     QKDALDRKIKEWAAQPGHDSYKQAIDKLEQLGAEQRRTARVDFDRRNAFESSRLLATA
                     LQLTRWADERTKKNEDRKAGFQDRDLNPSLAREKQFARVYDRTLDRAVFRLGLVRALQ
                     LPEADRPWLATLLDARKGARIDEALIDKTLDAWYAAQQLEDEKLRVSLLQSGTLAQLK
                     ASKDPFLQAAQRIYPTVKAEEKKADARTGDLLLVTPAYVEAMKQVLGGQLAPDANGSL
                     RITYGTVKSLRPESRDPADWPFTTATQILAKDTGKEPFNSPKKLLAAIKAKNYGPYAD
                     PSLGGELPIDFLSDLDITGGNSGSPTLNGKGELVGLAFDGNIEGVASDVVFNPATTRT
                     IQVDARYMIWTMDLLDGADH"
BASE COUNT          285 a          588 c          526 g          188 t
ORIGIN      
        1 agaacctcga gatccgcgac gtccgcctgg tctacgcgcc ggcgcgctcg gtcggcgact
       61 tcggcggcga ggtcgacaac tggcactggc cgcgccacac cggcgactgg tcgttctacc
      121 gggcctacgt cggcaaggac ggcaacccgg ccgactactc gcccgacaac gtcccgttcc
      181 accccaaggc ccacctccag gtgaccacgg ccgggctcaa gccgggcgac ttcgtgatgg
      241 tcaccggcta ccccggccgg accgaacgga cccggaccgc ggccgacatc caccacgagg
      301 tcgagtgggc gctgccctac cagatcgcgt acctcgagga gcgctacaag atcgccgagg
      361 cccacctcac cgatgacggc gagacccgga cccgggccac ggtgatgaag cagggcaccc
      421 agaacggcct cgagaaggac cgcggcgtgc tcgccgggct gacccggggc gatctgctcg
      481 cacagaagga cgcgctcgac cgcaagatca aggagtgggc ggcgcagccc ggccacgaca
      541 gctacaagca ggcgatcgac aagctggagc agctcggcgc cgagcagcgc cgcaccgcgc
      601 gggtcgactt cgaccgccgc aacgcgttcg agagctcgcg gctgctcgcc accgcgctgc
      661 agctgacccg gtgggccgac gagcgcacca agaagaacga ggaccgcaag gccggcttcc
      721 aggaccgcga tctcaacccg tcgctcgccc gtgagaagca gttcgcgcgg gtctacgacc
      781 gcacgctgga ccgcgcggtg ttccggctcg gcctggtccg cgcgctgcag ctgcccgagg
      841 ccgaccggcc gtggctcgcg accctgctcg acgccaggaa gggcgcccgg atcgacgagg
      901 cgctgatcga caagacgctc gacgcctggt acgccgcgca gcagctcgag gacgagaagc
      961 tgcgggtctc gctgctgcag tcgggcacgc tggcgcagct caaggcgtcg aaggatccgt
     1021 tcctgcaggc cgcgcagcgg atctacccca cggtcaaggc cgaggagaag aaggccgacg
     1081 cccgcaccgg cgacctgctg ctggtgacgc cggcctacgt cgaggcgatg aagcaggtgc
     1141 tcggcggcca gctcgccccc gacgccaacg gctcgctgcg catcacctac ggcacggtca
     1201 agtcgctgcg gcccgagtcc agggacccgg cggactggcc gttcaccacc gcgacccaga
     1261 tcctcgccaa ggacaccggc aaggagccgt tcaactcgcc caagaagctg ctcgcggcga
     1321 tcaaggccaa gaactacggt ccctacgccg acccgtcgct cggcggtgag ctgccgatcg
     1381 acttcctgtc cgacctcgac atcaccggcg gcaactccgg ctcgccgacg ctcaacggca
     1441 agggcgagct cgtcggcctg gcgttcgacg gcaacatcga gggcgtggcg tccgacgtcg
     1501 tgttcaaccc ggcgaccacg cgcacgatcc aggtcgacgc ccgctacatg atctggacga
     1561 tggacctgct cgacggcgcc gaccatc
//
LOCUS       DASVOR010002460         1886 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_495003,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002460 DASVOR010000000
VERSION     DASVOR010002460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1886
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495003"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="VF516_45090"
     CDS             <1..683
                     /locus_tag="VF516_45090"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEX8115010.1"
                     /translation="RPPPPATGPVRDAIAAAMARSKRDIPHYYLSHTISLQRAMAWLG
                     ELNAHRSIQDRMFPAVLLLRATALALWEVPELNGFWIDGAYRPSEQVHLGTAIALRTG
                     GLIAPAILDADDKPLDQLGVELRDLVARARAGRLRSSELTSATVTVTSLGDRGCEAVW
                     PVINPPQVAIVGFGAILQRPWALAGAVAAHPTVVATLAADHRVSDGHRGGLFLAAIDH
                     LLQEPDKL"
     gene            680..925
                     /locus_tag="VF516_45095"
     CDS             680..925
                     /locus_tag="VF516_45095"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HEX8115011.1"
                     /translation="MTDLAGPILATLHRIAPDVDPATVDRARPLVEQLDLDSIDYQNF
                     LAALATEHAIEIPERDVAGLRSIDDLVRYIAARAARA"
     gene            complement(1000..1134)
                     /locus_tag="VF516_45100"
     CDS             complement(1000..1134)
                     /locus_tag="VF516_45100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115012.1"
                     /translation="MTSLAGAAVQTVAGNGTGGYRDGDDPLTSELYGLEGLAVVPDGP
                     "
     gene            complement(1225..>1886)
                     /locus_tag="VF516_45105"
     CDS             complement(1225..>1886)
                     /locus_tag="VF516_45105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115013.1"
                     /translation="PPAREPLGAASGFAPGSWVRVKPAPALRELLDDRGRTRGLSFTQ
                     AQWDTAGEVFQVARQVRRLRDDHGRFRPISRTVLLDGVDCSGHGPEPAGCGRHCPMMY
                     RDEWLEAAPAPHRGPTQPSTERHARVRSVEEIRAGLDLRGRRDGLTFMPEMAQHAGKR
                     FAIASQLARVFEHDRWVTTRAPIYVLRGAQCSGTVVGDSGPCDRACALMWHEDWLIVE
                     S"
BASE COUNT          242 a          726 c          657 g          261 t
ORIGIN      
        1 gccggcctcc gccgcctgcg accggtcccg tgcgcgatgc gatcgccgcg gcgatggcgc
       61 gctccaagcg cgacattccg cactactacc tctcgcacac gatcagcctc cagcgcgcga
      121 tggcctggct cggcgagctc aacgcgcacc gctcgatcca ggaccgcatg ttccccgccg
      181 tgctgctgct ccgcgcgacc gcgctcgcgc tgtgggaggt tcccgagctc aacggtttct
      241 ggatcgatgg cgcgtaccgc ccgtccgagc aggtccacct cggcaccgcg atcgcgctgc
      301 gcaccggcgg gctgatcgcg ccggcgatcc tcgacgccga tgacaagccg ctcgaccagc
      361 tcggggtcga gctgcgcgat ctggtcgcgc gtgcccgggc gggccggctg cgcagctccg
      421 agctgacgtc ggcgacggtc accgtgacca gcctcggcga ccgcggctgc gaggcggtgt
      481 ggccggtgat caacccgcct caggtcgcga tcgtcggatt cggcgcgatc ctgcagcggc
      541 cgtgggccct ggccggcgcg gtcgcggctc atccgacggt ggtcgcgacg ctcgcggcgg
      601 atcaccgcgt gtccgacggc caccgcggcg ggttgttcct cgccgcgatc gaccacctgc
      661 tgcaggagcc cgacaagcta tgaccgatct cgccggcccg atcctcgcca cgctgcatcg
      721 catcgcaccc gacgtcgatc ccgccacggt cgaccgggcg cgaccactcg tcgaacagct
      781 cgatctcgac tcgatcgact atcagaactt tctggccgcg ctggccaccg agcacgccat
      841 cgagatcccc gagcgagatg tcgcgggcct gcgctcgatc gacgatctgg tgcgctacat
      901 cgccgcgcgc gccgcgcggg cgtgatcacc ccacggcgga gttctggcgc gcgaccggtc
      961 cgccttgcct tgcaacccct gcttcgtcgg cgacgtagat cagggcccgt cgggcaccac
     1021 ggcgagcccc tcgaggccgt agagctcgga ggtcagcgga tcgtcgccgt cgcgatagcc
     1081 gccggtgccg ttccccgcga cggtctggac ggccgcgccc gcgagcgacg tcaccgcgcc
     1141 ggtcgcgcga tcgcgcagtt cgcgcgtgcg acgcagtcgg tcctgatcgg attgcgcgcg
     1201 gaggaccgga cgcgccgcgc cgagtcacga ctcgacgatc agccagtcct cgtgccacat
     1261 gagcgcgcac gcccggtcgc acggaccgct gtcgccgacc acggtgcccg agcactgcgc
     1321 gccgcgcagg acgtagatcg gcgctcgcgt cgtgacccag cgatcgtgct cgaacaccct
     1381 cgccagctgg ctcgcgatcg caaaccgctt gcccgcgtgc tgcgccatct cgggcatgaa
     1441 tgtcagcccg tcgcggcggc cgcgcaggtc gaggccggcg cggatctcct ccacgctgcg
     1501 cacccgggca tgccgctcgg tgctcggctg ggtcgggccg cggtgtggtg ccggtgcggc
     1561 ctcgagccac tcgtcgcggt acatcatcgg gcagtggcgc ccgcacccgg cgggctcggg
     1621 gccatggccc gagcagtcga cgccgtcgag cagcacggtg cgcgagatcg gccggaaccg
     1681 gccgtgatcg tcgcgcagcc gccgcacctg ccgggcgacc tggaacacct cgccggcggt
     1741 gtcccactgc gcctgcgtga acgaaaggcc ccgcgtgcgg ccgcggtcgt cgagcagctc
     1801 gcgcagcgcg ggggccggct tgacgcgcac ccagctgccg ggggcgaacc ccgacgctgc
     1861 acccagcggc tcgcgcgcgg gcggcg
//
LOCUS       DASVOR010002461         7317 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_231805,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002461 DASVOR010000000
VERSION     DASVOR010002461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231805"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VF516_45110"
     CDS             <1..1164
                     /locus_tag="VF516_45110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115014.1"
                     /translation="EASLAKLVPIAISLFADLIGLGGIADKIRGIIEKVQTKVDQAID
                     KLITRVMAMFKGKKGDKPDDKPDDRTPEQKQADLDKAVAEVKALGESNALSGDKLDGE
                     LDSIKTKYRLTELKVQKKGKGGELQIHAAVNPEATFELFAIKDDASALREAVGNQLSS
                     LPPKATIAQVKALCAQAAQDHLEPGFKLDWQDMRDSWVLFLERPKHPIPRTAVGEFRQ
                     AINYTKVSSHGNQTLYKDEAGNEYVNDSIDQYVPRFLTRNISEQDRKSLKQGIKPTAP
                     KRKLSPKEHVMGYKPSPFISTTKLDGDQIENPHGDMLGGEHGTVRIDLSLISSKVIFD
                     LTTRRGQDTWNLSNPEFTKGAKAGTSRQALEDVVRTQEVLIKGDIPPEAIELL"
     gene            1161..1907
                     /locus_tag="VF516_45115"
     CDS             1161..1907
                     /locus_tag="VF516_45115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115015.1"
                     /translation="MMFDSYYWHAGRVRPELEQARERLAAGHDRAAFKLLLRSGDPVA
                     VAIALDHYHRADASTRHGTPNPFEDHRDEVIAVARDVLRGAPTEEREDIAAGANYASA
                     LGALMNLAEPQDAVLIVAALERVSTSELRFAAAAAASTLLERSPDPDERLIAALEAVA
                     LDDTAGRDAREAAASAVGRTRSAAATHAVLRFLDTGEFWLQAVAALHLLDIDRRAYRA
                     RVEQIVRGWPEPPPYPGDEVLELLAEADAT"
     gene            complement(2048..2950)
                     /locus_tag="VF516_45120"
     CDS             complement(2048..2950)
                     /locus_tag="VF516_45120"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEX8115016.1"
                     /translation="MNITATNLNLFVAFDALIEHGSVSRAARHVGVTQSAMSNSLRNL
                     RAVFGDPLFLRMSHGIVPTPRARQLAGPVREAMRLLERALGPLQFEPAASSRTFTLLA
                     SDHVEFVLLPRLLGYLGNHAPGVRLRVLPWGLQRVPDDLARGDADLMIGFYDRVPAHH
                     REQLLFDERYACIVRKGHPMVGRRLTLRTYVAMKHIMVSQSAEATSGIDRALAALGRT
                     REVSLRVSHFLNVPTLVASSDLVASLSRRVAEPFARMLPLQLFEPPLRLRTSRIGMVW
                     HDSLHDDPAQRWLRGAIAELCAQL"
     gene            3017..3520
                     /locus_tag="VF516_45125"
     CDS             3017..3520
                     /locus_tag="VF516_45125"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEX8115017.1"
                     /translation="MANQPSLLHRALSTDQDRTLLAQRVALGLIMFPHGAQKLLGWFG
                     GYGWSGTMAFFTDTMHLPAPLAFLIIVAESLGAAGLVLGAGTRLAALGIASVMLGAIF
                     TTHLGVGFFMNWSGAQHGEGFEYHLLALALALPLVVRGGGARSVDGALAARLSSGGFA
                     VPQPSQA"
     gene            3526..4197
                     /locus_tag="VF516_45130"
     CDS             3526..4197
                     /locus_tag="VF516_45130"
                     /inference="COORDINATES: protein motif:HMM:NF012273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8115018.1"
                     /translation="MLTLVSHHLCPYVQRVAIALVEKGAAFDRVHVDLAAKPAWFLAQ
                     SPLGKTPLLNVDGDVLFESAAICEYLEDALPHPLHPEAPIERARHRAWMELGSAVLGD
                     IWQLETAQDATGIERAAGALRDKLGRLEAQLGAEPYFAGSRFHLVDAVFAPAFRYFDV
                     FDELADLGVFDALPRVRAWRAALALRPSVRDAVPADYAERLRMFLHSKNAYLWTHRAD
                     RSHRS"
     gene            4357..4623
                     /locus_tag="VF516_45135"
     CDS             4357..4623
                     /locus_tag="VF516_45135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115019.1"
                     /translation="MSERAELERRADVVILTALRLEYDAVLQVDAGAVHGSTWEHAKG
                     PSGLPLAFRPFEVSTGRPLRVAAAVAADMGATAATNTLLHWSRR"
     gene            complement(4905..5528)
                     /locus_tag="VF516_45140"
     CDS             complement(4905..5528)
                     /locus_tag="VF516_45140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115020.1"
                     /translation="MTWPWITLLLLGAWHGVNPGMGWLFAVSRGLQERRGGAVVGALP
                     PIALGHALAIAVALVVVAIGRASVPLVALRWATAAILIGFGISRLFRHHHPRWVGMRV
                     GFRDLTVWSFLMATAHGAGLMLAPLFVVDGARPSCHADAGLTLSGPIDYVVATAAHTA
                     AMLVVAGGIALVVYYKLGLALLRRAWFNLDWLWALALVGAGLFAALA"
     gene            complement(5525..6925)
                     /locus_tag="VF516_45145"
     CDS             complement(5525..6925)
                     /locus_tag="VF516_45145"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HEX8115021.1"
                     /translation="MTLRPDPSFYPSPRLAGEAPPEKLAYVAAIDPAGIAGAKGGEPD
                     ALAVVDLDPASSGYGTISSLAEMPNVGDELHHFGWNACSASLCPWAPNPHVERRYLLV
                     PGLRSSRIHVFDVKPNPRKPTLVKTIEPGEIAHRTGYSRPHTIHCGPDGLYVNALGNP
                     EGDGPGGIFILDCESFDVRGRWEVDRGPQELAYDFWWHLGYDTLLSSEWGTPKMVESG
                     VIPELLLGKKYGHRVHVWDLRKRRHVQALDLGDEHQMVLELRPAHDPTKAYGFVGVVV
                     SVADLSASVWTWYREGDRWAIQKTITIPAVPAEASKLPPLLQGFGAVPPLVTDLNLSL
                     DDKFLYVSCWGTGELKQYDVSNPLAPREVGSVAIGGIVNRTAHPRTGPLTGGPQMVEI
                     SRDGKRVYLTSSLYASWDAQFYPDGVAGWMTKIDVNQSGGMAIDPRFCIDFGRRRPHQ
                     VRLEGGDASSDSYCYP"
     gene            7057..>7317
                     /locus_tag="VF516_45150"
     CDS             7057..>7317
                     /locus_tag="VF516_45150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115022.1"
                     /translation="MGSPQDDTKSGASDRHAPAPDTAGSPVPDPPARAKSESQPRPRP
                     RSIPPPVPRERGKRPTLVSIPSRPESPSVVDRALDRLAQLDDV"
BASE COUNT         1184 a         2557 c         2534 g         1042 t
ORIGIN      
        1 gaggcctcgc tcgccaagct ggtcccgatc gcgatcagcc tgttcgccga cctgatcggc
       61 ctgggcggca tcgccgacaa gatccgcggc atcatcgaga aggtccagac caaggtcgac
      121 caggcgatcg acaagctgat cacccgcgtg atggcgatgt tcaagggcaa gaagggcgac
      181 aaacccgacg acaagcccga cgaccgcacc cccgagcaga agcaggctga tctcgacaag
      241 gccgtcgcag aggtcaaggc gctgggggag agcaacgcac tgagcggtga caagctcgac
      301 ggcgagctcg acagcatcaa gacgaagtac cggctcaccg agctcaaagt gcagaagaag
      361 ggcaagggcg gtgagctcca gatccatgca gcggtcaatc ccgaggctac cttcgagctg
      421 ttcgcgatca aggatgacgc atccgcattg cgcgaggcgg tcggaaacca gctgagctca
      481 ctaccgccca aggccacgat agcgcaggtc aaggcgctgt gcgcccaggc ggcccaggac
      541 cacctggagc ccggcttcaa gctggactgg caggacatgc gcgactcgtg ggtgctgttc
      601 ctcgagcgcc ccaagcaccc gattccacgc accgcggtcg gcgagttccg ccaggcaatc
      661 aactacacga aggtcagctc gcatggtaat cagacactct acaaggacga agccggtaat
      721 gagtatgtga acgattcgat cgaccagtac gtcccgcggt tcctgacgcg caacatctcc
      781 gagcaagacc ggaaatcgct caagcagggc atcaagccca ccgcgccgaa gcgcaagctc
      841 agccccaagg agcacgtcat ggggtacaag ccatcgccgt tcatctccac cacgaagctc
      901 gatggcgatc agatcgagaa cccgcacggc gacatgctcg gcggcgagca cggcacggtc
      961 cggatcgacc tgtcgctgat ctcatccaag gtcatcttcg acttgacgac ccgccgcggc
     1021 caggatacgt ggaacctgag caaccccgag tttaccaagg gcgccaaggc aggcacgtcg
     1081 cgccaggcgc tggaggatgt cgtgcgaacg caggaggttc tgatcaaggg tgacattccg
     1141 cctgaagcga tcgagctgct atgatgttcg actcctacta ctggcacgcg gggcgcgtga
     1201 gacccgagct ggaacaggcc cgggagcggc tggccgctgg ccacgatcgg gccgcgttca
     1261 agctgctgct gcgcagcggc gatccggtcg cggtcgcgat cgccctcgat cactatcacc
     1321 gcgcggatgc gtccacgcgc cacggcacac cgaatccgtt cgaggaccac cgcgacgagg
     1381 tcattgcagt agcgcgcgac gtgctgcgcg gcgcaccgac cgaggagcgc gaggacatcg
     1441 ccgcgggtgc gaactacgcc agtgctctcg gcgcgctgat gaacctggct gaacctcagg
     1501 acgcggtgct catcgtggct gcgctcgagc gcgtgtccac ttcggagctg cgcttcgcag
     1561 ccgctgccgc tgcgagcacg ttgctcgagc gttcgccgga tcccgacgaa cggctgatcg
     1621 cggcactcga ggcggtcgcg ctcgacgaca cggcgggtcg cgacgcccgc gaagctgccg
     1681 cctccgcggt aggacgcacg cggtcggctg ccgcgaccca tgcggtgctc cgcttcctgg
     1741 acactgggga attttggctc caggcagtgg cggcgctgca cctgctcgac attgaccggc
     1801 gcgcctatcg cgctcgcgtc gagcagatcg tgcgcggctg gccggagccg ccgccgtatc
     1861 ccggcgacga ggtcctcgag ttgctcgccg aggccgacgc gacctgacgt gcgttgcgcc
     1921 ctacctcacc accgaatcgc tggacggatc gagcaacatc catggcgggt ttgaccgcga
     1981 cccaggccga ggtcgcgagg cggtttctga ccgccatggc gaacctgcgg gaggctcgcc
     2041 ggtccactca cagctgcgca cagagctcgg cgatcgcgcc gcgcagccag cgctgtgcag
     2101 gatcgtcgtg cagcgagtcg tgccacacca tcccgatccg gctcgtccgc agccgcagcg
     2161 gcggctcgaa cagctgcagg gggagcatcc gggcgaacgg ctcggcgacc cgacggctca
     2221 gcgacgccac gagatcgctg ctcgcgacca gcgtggggac gttcaggaaa tgggacaccc
     2281 gcaggctcac ctcccgcgtc cgtcccagcg cggcgagcgc gcgatcgatg ccgctggtcg
     2341 cctcggcgct ctgcgacacc atgatgtgct tcatcgccac gtaggtccgc agcgtcaagc
     2401 ggcggcccac catcgggtgg cccttgcgga cgatgcacgc atagcgctcg tcgaacagca
     2461 gctgctcgcg gtggtgcgcc gggacccggt cgtagaaccc gatcatgagg tcggcgtcgc
     2521 cgcgcgcgag atcgtcggga actcgctgca gcccccacgg cagcacccgg aggcgcacac
     2581 cgggtgcgtg gttgcccagg tagccgagca gccgcggcag cagcacgaac tcgacatggt
     2641 cgctcgcgag cagggtgaac gtccgggacg acgccgccgg ctcgaactgc agcgggccca
     2701 gcgcgcgctc gagcagccgc atcgcctctc gcaccgggcc ggcgagctgg cgggcccggg
     2761 gcgtcggcac gatgccatgc gacatgcgga ggaacagagg atccccgaac accgcgcgga
     2821 gattgcggag cgagttgctc atcgccgact gcgtgacccc gacgtggcgg gccgcccggc
     2881 tgacgctgcc gtgctcgatc agcgcatcga acgcgacgaa caggttgagg ttagtcgcgg
     2941 tgatattcat tgtatcgata tcttacatca caaacatgcg ctttgaagat gccaccggcg
     3001 gacccagaat ggcggcatgg cgaaccaacc ttccctcctc catcgcgcac tgtcgaccga
     3061 ccaggaccgg acgctgctgg cccagcgcgt cgcactcggg ctgatcatgt tcccccacgg
     3121 ggcccagaag ctgctgggct ggttcggagg gtacggctgg tccggcacga tggcgttctt
     3181 caccgacacg atgcacctcc cggcgccgct cgcgttcctg atcatcgtcg ccgagtcgct
     3241 gggagccgcc gggctggtcc tcggcgccgg tacgcggctg gcggcgctcg gcatcgcctc
     3301 ggtgatgctc ggcgcgatct tcacgactca cctgggtgtc ggcttcttca tgaactggtc
     3361 cggcgcccag cacggggaag gcttcgagta ccacctgctc gcgctcgcgc tcgcgctgcc
     3421 gctcgtcgtg cgaggcggcg gtgcgcggtc ggtcgacggt gcgctcgcgg cgcggctgtc
     3481 gtcgggcggc ttcgccgtgc cgcaaccgag ccaggcgtga ccaccatgtt gaccctggtc
     3541 agtcatcacc tctgcccgta cgtccagcgc gtcgcgatcg cgctcgtcga gaagggcgcc
     3601 gcgttcgacc gcgtccatgt cgatctggcc gccaagccag cgtggttcct cgcccagtcg
     3661 ccgctcggca agacgccgct gctgaacgtc gacggcgacg tgctgttcga atccgccgcg
     3721 atctgcgagt acctcgagga tgcgctgccg cacccactgc atcccgaagc gccgatcgag
     3781 cgcgcgcggc atcgcgcatg gatggagctc ggctcggccg tcctcggcga tatctggcag
     3841 ctggagaccg cacaggacgc caccggcatc gaacgcgccg ccggcgcgct ccgcgacaag
     3901 ctcgggcggc tcgaggccca gctcggcgcg gagccgtact tcgcgggcag caggttccac
     3961 ctcgtcgacg ccgtgttcgc ccccgcgttc cgctacttcg acgtcttcga cgagctcgcc
     4021 gacctcggcg tgttcgacgc gctgcctcgg gtccgcgcat ggcgcgccgc gctcgccctg
     4081 cggccgagtg tccgcgatgc cgtgcccgcc gactacgcag agcggctccg gatgttcctc
     4141 cacagcaaga acgcatacct ctggacccac cgcgccgacc gatctcaccg ttcgtgagta
     4201 cgacccgcgc cagcgggggc tgaaccggtt catcaccgcg accggaacgc cctcacgcgg
     4261 gcgattgcgg taccctcgcg gcgtggggag cgcctggctc caggatcggt cgagatgcgc
     4321 gacgttcgct gaggcgatct gcaggcgagc gaccgcatga gcgagcgggc agagctggag
     4381 cgtcgcgccg acgtggtcat cttgaccgcg ctccggctcg agtacgacgc ggtgctgcag
     4441 gtcgatgccg gcgcggtgca cggctccacc tgggagcatg cgaagggccc gagcgggctg
     4501 ccgctggcgt tccggccgtt cgaagtgtcg accggccggc cgctgcgcgt cgccgcggct
     4561 gtcgcagccg acatgggggc cactgcagcg accaacacgc tcctgcactg gtcgaggcgc
     4621 tgaagccgcg ctgcgtcgcc atgtgcggcg tgtgcgccgg ccggccggag aagacacagc
     4681 tcggagatgt cgtatgggtg caaaccgcgg tcgtgtggtt ggtccagctg cgcccgtacg
     4741 cagtacccct tcagggcggc agaccggtga gcctaccggc tggtagcgtc ggcggcggcg
     4801 atcggcggtc gggttgcggc gttcggccat cggtcgtcgg cgttcggccg tcggcgctcg
     4861 gcgttcggtc gtcggcgttc gtgaatgcgg cgctcagccg ctcgctacgc cagtgccgcg
     4921 aacaacccgg cgccgaccag cgccagcgcc cacagccagt cgagattgaa ccacgcgcgc
     4981 cgcagcagcg cgagcccgag cttgtagtac acgaccagcg cgatcccccc cgcgacgacg
     5041 agcatcgccg cggtgtgcgc cgcggtggcc acgacatagt cgatcggccc cgacagcgtc
     5101 agcccggcgt cggcgtggca cgacggccgc gcgccatcga ccacgaacag cggcgccagc
     5161 atgagcccgg cgccgtgcgc cgtcgccatc aggaacgacc acacggtgag atcgcggaac
     5221 ccgacccgca tgccgaccca gcgcgggtgg tggtggcgga acagccgcga gatgccaaag
     5281 ccgatcaaga tcgccgcggt ggcccagcgc agcgcgacca gaggcacgct ggcgcggccg
     5341 atcgcgacga cgacgagggc gaccgcgatc gccagcgcgt ggccgagcgc gatcggcggc
     5401 agcgcaccga cgaccgcgcc gccccgccgc tcctgcagcc cgcgcgacac cgcgaacagc
     5461 cagcccatgc cgggattcac gccgtgccag gcgccgagca ggagcagcgt gatccacggc
     5521 caggtcatgg gtagcagtac gaatcggacg acgcgtcacc gccctcgagg cggacctggt
     5581 gcgggcggcg gcggccgaag tcgatgcaga acctgggatc gatcgccatg ccgccgctct
     5641 ggttgacgtc gatcttggtc atccagcccg ccacgccatc gggatagaac tgcgcgtccc
     5701 acgacgcgta cagcgagctg gtcaggtaga cccgcttgcc gtcgcggctg atctcgacca
     5761 tctgcgggcc gccggtcagc gggccggtcc tggggtgggc ggtgcggttg acgatgccgc
     5821 cgatcgcgac cgagccgacc tcccgcggcg ccagcgggtt ggacacgtcg tactgcttga
     5881 gctcgccggt gccccagcac gacacgtaga ggaacttgtc gtcgagcgac aggttgaggt
     5941 cggtgaccag cggcggcacc gctccgaacc cctggagtag cggcggcagc ttggacgcct
     6001 cggccggcac cgccgggatc gtgatggtct tctggatcgc ccagcgatcg ccctcgcggt
     6061 accaggtcca gaccgaggcg gacaggtcgg cgacgctgac caccacgccg acgaagccgt
     6121 aggccttggt cggatcgtgg gcgggccgga gctcgagcac catctggtgc tcgtcgccga
     6181 gatcgagcgc ctggacgtgg cgccgcttgc gcaggtccca gacgtggacc cggtggccgt
     6241 acttcttgcc gagcaggagc tcggggatca cgccgctctc caccatcttc ggcgtgcccc
     6301 actcgctcga cagcaaggtg tcgtagccga ggtgccacca gaagtcgtag gcgagctcct
     6361 gcggaccgcg atcgacctcc cagcggccgc ggacgtcgaa gctctcgcag tcgaggatga
     6421 agatgccgcc gggaccgtcg ccctcggggt tgcccagcgc gttgacgtac agcccgtcgg
     6481 ggccgcagtg gatcgtgtgc ggccgcgagt agccggtgcg gtgggcgatc tcgccgggct
     6541 cgatggtctt gaccaacgtg ggctttcggg ggttcggctt gacgtcgaag acatggatcc
     6601 gcgacgagcg cagccccggc accagcaggt agcgccgctc gacgtgcggg ttgggcgccc
     6661 acgggcacag cgacgcgctg cacgcgttcc acccgaagtg gtggagctcg tcgccgacgt
     6721 tgggcatctc ggccagcgac gagatcgtgc cgtagcccga ggacgccggg tcgaggtcga
     6781 cgaccgcgag cgcgtcgggc tcgccgccct tggcgcccgc gatccccgcc ggatcgatgg
     6841 cggcgacgta cgcgagcttc tccggcggtg cttccccggc cagacgcggc gatggataga
     6901 aggagggatc gggacgtagg gtcatggtgc gctccgggcg catcatatag agggccgcgt
     6961 ggtgaggcca cagggggggc gccgacgcgc cgagccccgc cggcggcccg gcgcgccgat
     7021 tgacactccc gggctcccgg tgatgatctg gatgggttgg ggtcccccca ggacgatacg
     7081 aagtctggag cgagcgaccg ccacgcgccg gccccggaca cggcgggctc gccggtgccc
     7141 gatcccccgg cgcgcgcgaa gtccgagagc cagccgcgtc cgcggccgcg gtcgattccc
     7201 ccgccggttc cccgcgagcg cggcaagcgc cctaccctgg tctcgatccc gagccgcccg
     7261 gagtcgccca gcgtcgtgga tcgcgcgctc gaccggctgg cgcagctcga cgacgtc
//
LOCUS       DASVOR010002462         5190 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_57218,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002462 DASVOR010000000
VERSION     DASVOR010002462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5190
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57218"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1402)
                     /locus_tag="VF516_45155"
     CDS             complement(<1..1402)
                     /locus_tag="VF516_45155"
                     /inference="COORDINATES: protein motif:HMM:NF017009.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115023.1"
                     /translation="MPRLGELLVAAGLLTPEQVEQALRAQVLWGGRLGTNLVELHYLE
                     LDPLSRVLGRQHHLPAALARHFEKADPELQARLPPELAERFSCLPLLRMGPDPHVVIA
                     AMAPLTPRQLAVIAGELAVDVARLVPAVAAELRIRYQLERVYGIQRAARYLRARGVSI
                     PLFPAFRIPPVQSDAEIDLSTPSLPVSTREIEAYRPDPTDAPTRAPAPTPAVDRRETG
                     GEAPCGIDRTPTEPDVAAALTALGAMPDEGQDEPPDDGGEDDLIPVEVEIEVEIEVEI
                     EIDAPAAPDDCADPGLEDDLAIPGEPVEDEAQARERRHYVRTIADAASADAASDDAVS
                     DDDAASDDAASDDDRQSTLGRIAIRRVAVGSAPRVMAGATLGEATRAIRRGTARDRVA
                     DLVIDTLDRFAPACEAAILLVVRGATAIGWKGFQRAGGGLPEIAVPMDDAGLVPRAVE
                     LNVSLRRAATELDRIDL"
     gene            complement(1531..2592)
                     /locus_tag="VF516_45160"
     CDS             complement(1531..2592)
                     /locus_tag="VF516_45160"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HEX8115024.1"
                     /translation="MELRKIREILGDQAALLEHRSQTIPASMLTLPSPSYLDDVFAQS
                     DRTPRVLGAMARMRSHGRLANTGYVSILPVDQGIEHSAGASFAPNPQMFDPENIVRLA
                     IEGGCSAVASTYGVLGMVARKYAHKIPFIVKMNHNELMSYPNTYDQTLFGDVRQCFDM
                     GAQGVGATVYWGSNESRRQLHEISLLFAEAHRLGMFTVLWCYVRNNAFKTALGDHSLS
                     ADLTGQANHLGVTIQADIIKQKLPESNGGYNDPALKGFGKTHKKVYSELTTDNPIDLT
                     RYQVANCYMGRIGLINSGGASGENDLREAVMTAVINKRAGGMGLIAGRKAFQKPFKEG
                     VELLHAIQDVYLAKEITVA"
     gene            2750..3544
                     /locus_tag="VF516_45165"
     CDS             2750..3544
                     /locus_tag="VF516_45165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEX8115025.1"
                     /translation="MTPESNQPAVAPDPGGRRAWRDAVLAFAVVEVLVTGLVRIRVTL
                     PWIGNLGAALVVIVFLYVPFHVAGRHNEDLADYGFRAEPIGRGLAIAAVAVAVIVPVF
                     TAVFLGFYELVCRSELLSHLAPHQMCSHYAGLAGLHGPHLVWREPPPPVTVLSVEWCA
                     SQWLVVGLSEELFFRGFLLEQLERRFPPRRRILGGGVGLALVLSAVAFALIHLPKDGD
                     PRDLATFFPGLLFGWMRSATRSILPSTITHGCCNILARTLELAVSR"
     gene            3541..4227
                     /locus_tag="VF516_45170"
     CDS             3541..4227
                     /locus_tag="VF516_45170"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEX8115026.1"
                     /translation="MIDGDRSGDRPSRQAAAFFDMDNTLLRVDSGMSWVRFLYRRGEL
                     PPRMVAKALYWQALYKLAVLDMDAVFTRLVEDLRGDSEAEMIAKCAIWYRDHVAPEVA
                     PAARVAIEHHRRAGHVIVLATGSTQYAARPVAHALGIEHVLSSVLEVEGGVFTGRPAA
                     FCFGHHKVALAERWADRHDIDLAASYFDSDSYNALPMLERVGTAIAVNPDARLRRHAR
                     RRGWATPRWA"
     gene            complement(4397..4789)
                     /locus_tag="VF516_45175"
     CDS             complement(4397..4789)
                     /locus_tag="VF516_45175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115027.1"
                     /translation="MATSEDLPAARTACVVVGAAPEQVAAVGTNARTTLRANKLGTLV
                     TGRPGDLVIDYAAELPGPVYDVMYSAQTGWFSVTVFRGLDPPVRWDNRPGTDPGYTRI
                     GDVLGATSPTMILAALDIPAIALGYHGA"
     gene            4900..>5190
                     /locus_tag="VF516_45180"
     CDS             4900..>5190
                     /locus_tag="VF516_45180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115028.1"
                     /translation="MTTPVLGGLAALPAVRRLIELALDEDLGRGDATSRAVFGGAVPG
                     AVPVAVPRVVADMVARDPIVAFGIEVAAAVFARVDPAIAVEAGCPDGSHVDRG"
BASE COUNT          669 a         1813 c         1893 g          815 t
ORIGIN      
        1 gcaggtcgat ccgatcgagc tccgtggcgg cgcgccgcag cgagacgttg agctcgaccg
       61 cgcgcggcac caggccggcg tcgtccatcg gcaccgcgat ctcgggcagc ccgccgccgg
      121 cgcgctggaa gcccttccag ccgatcgccg tcgcgccgcg gaccaccagc aggatcgcgg
      181 cctcgcacgc cggggcgaag cggtcgagcg tgtcgatcac caggtcggcg acccgatcgc
      241 gggcggtgcc gcgccggatc gcgcgggtcg cctcgccgag cgtggccccg gccatcaccc
      301 gcggggcgga gccgaccgcg accctgcgga tcgcgatccg gccgagcgtc gactggcgat
      361 cgtcgtcgga cgccgcgtcg tcggacgccg cgtcgtcgtc ggacaccgca tcgtcggatg
      421 ccgcgtcggc ggacgccgcg tcggcgatcg ttcgaacata gtgccggcgc tcgcgcgcct
      481 gggcctcgtc ctcgaccggc tcgccgggga tcgcgagatc gtcctccagc cccgggtccg
      541 cgcagtcgtc cggcgcggcc ggcgcatcga tctcgatctc gacctcgatc tcgacctcga
      601 tctcgacctc caccgggatc aggtcgtcct cgcccccgtc gtccggtggc tcatcctggc
      661 cctcgtcggg catcgccccg agcgcggtca gcgccgccgc cacgtcgggc tcggtcggtg
      721 tgcggtcgat cccacagggg gcttcgcccc ccgtctccct gcggtcgacc gcaggcgtcg
      781 gcgccggcgc gcgggtcggg gcgtcggtcg gatccggacg gtacgcctcg atctcgcggg
      841 tcgacaccgg caggctcggc gtcgagagat cgatctcggc gtcggactgg accgggggga
      901 tccggaacgc cgggaacagc gggatgctca cgccgcgcgc ccggaggtac cgcgccgcgc
      961 gctggatccc gtagacgcgc tcgagctggt agcggatccg cagctcggcg gcgacggccg
     1021 ggaccagccg cgcgacgtcg accgcgagct cgccggcgat gaccgcgagc tggcgcgggg
     1081 tgagcggagc catcgcggcg atcacgacat gcggatcggg gcccatccgg agcagcggca
     1141 ggcacgagaa ccgctcggcg agctccggcg gcagccgggc ctggagctcg gggtcggcct
     1201 tctcgaagtg gcgggcgagc gcggcgggca ggtggtgctg gcggccgagc acccgggaca
     1261 gcggatcgag ctccaggtag tgcagctcga cgaggttggt gccgaggcgt ccgccccaca
     1321 gcacctgggc gcgcagcgcc tgctcgacct gctcgggggt gagcagcccg gccgcgacga
     1381 ggagctcccc gagcctgggc atctaccccc actgtatcag cctggaccgc ctcctctaaa
     1441 atgactggac cgcctcctct acaatgactg gaccgcctcc tctaaaatga ctggaccgcc
     1501 tcctctaaaa tgaggcgtgc cgcgcaaagc tcaggcgacg gtgatctcct tcgccaggta
     1561 cacatcttga atcgcgtgga gtagctcgac gccctccttg aacggcttct ggaacgcctt
     1621 gcgcccggcg atcaggccca tgccaccggc gcgcttgttg atcaccgcgg tcatcaccgc
     1681 ctcgcgcaga tcgttctcac ccgaggcgcc gccgctgttg atcaggccga tcctccccat
     1741 gtagcagttg gcgacctggt agcgcgtgag gtcgatcgga ttgtcggtgg tgagctcgga
     1801 gtagaccttc ttgtgggtct tgccgaagcc cttgagcgcg gggtcgttgt agccgccgtt
     1861 gctctcgggg agcttctgct tgatgatgtc ggcctggatc gtgacgccga ggtggttggc
     1921 ctggccggtg aggtcggcgg acaggctgtg gtcgccgagg gcggtcttga acgcgttgtt
     1981 ccgcacgtag caccacagca ccgtgaacat gccgagccgg tgagcttcgg caaacaggag
     2041 agagatctcg tggagctggc ggcgcgactc gttcgatccc cagtacacgg tggcgccgac
     2101 cccctgtgcg cccatgtcga agcactggcg cacatcgccg aacagcgtct ggtcgtacgt
     2161 gttggggtac gacatcagct cgttgtggtt catcttcacg atgaacggga tcttgtgggc
     2221 gtacttgcgc gcgaccatgc cgagcacgcc gtaggtcgac gcgaccgcgc tgcagccgcc
     2281 ctcgatcgcg agccgcacga tgttctcggg atcgaacatc tgcgggttgg gggcgaacga
     2341 cgcgccggcc gagtgctcga tgccctggtc gaccggcagg atcgacacgt agccggtgtt
     2401 ggcgagccgg ccgtgtgacc tcatccgcgc catcgcgccg agcacgcgcg gcgtgcgatc
     2461 gctctgcgcg aacacgtcgt cgaggtagct cggcgacggc agggtgagca tgctggccgg
     2521 gatggtctgg gaccggtgct cgagcagcgc ggcctggtcg ccgaggattt cgcggatctt
     2581 gcggagctcc atggcctggt gtatcgcgcg ctcgccgagc gggcgcaagc ggtcgtcgac
     2641 cgcagcgggg accctggcgg agggcgcccg atgccgcgcg gccgcgccgg gtgccgatgt
     2701 gcgcggccgc tcgcggaaac cgccgcagat cgggtacggt taggcggcgg tgacgccgga
     2761 gtcgaaccag cctgcggtcg cgccggatcc gggcggccgg cgcgcgtggc gcgacgccgt
     2821 gctggcgttc gcggtggtgg aggtgctggt caccgggctg gtccggatcc gcgtgacgct
     2881 gccgtggatc ggcaacctcg gcgcagcgct ggtcgtgatc gtgttcctct acgtgccgtt
     2941 ccatgtcgcg ggccggcaca acgaggatct cgccgactac gggttccgcg ccgagccgat
     3001 cggccgcggc ctggcgatcg cggcggtcgc ggtggcggtg atcgtgccgg tgttcacggc
     3061 cgtgttcctc gggttctacg agctcgtgtg ccgctccgag ctgctctcgc acctggcgcc
     3121 gcaccagatg tgcagccact acgccgggct cgccggcctg cacgggcccc acctggtgtg
     3181 gcgggaaccc ccgccgccgg tcaccgtgct gagcgtcgaa tggtgcgcgt cgcagtggct
     3241 ggtcgtcggg ctgtccgagg agctgttctt ccgcggcttc ctgctcgagc agctggagcg
     3301 ccggttcccg ccgcggcggc ggatcctcgg cggcggcgtc ggcctggcgc tggtgctgtc
     3361 ggccgtggcg ttcgcgctga tccacctgcc caaggacggc gatccgcgcg atctggccac
     3421 gttcttcccc ggcctgctgt tcggctggat gcgctcggcg acccggtcga ttctcccgtc
     3481 gacgatcacc cacggctgct gcaacatcct ggcgcgcacg ctggagctgg cggtgtcgcg
     3541 atgatcgacg gcgatcgatc gggcgaccgt ccgagccggc aggcggcggc gttcttcgac
     3601 atggacaaca cgctgctgcg ggtcgactcc ggcatgagct gggtgcgctt cctgtaccgg
     3661 cgcggcgagc tgccgccgcg gatggtcgcc aaggcgctgt actggcaggc gctgtacaag
     3721 ctcgcggtgc tcgacatgga cgcggtgttc acccggctgg tcgaggacct gcgcggcgac
     3781 tccgaggccg agatgatcgc caagtgcgcg atctggtacc gcgaccacgt cgcgcccgag
     3841 gtcgcgcccg ccgcgcgggt cgcgatcgag caccaccgcc gggccggtca cgtcatcgtg
     3901 ctcgccaccg gctcgaccca gtacgccgcg cgcccggtcg cccacgcgct cggcatcgag
     3961 cacgtgctgt cctcggtgct cgaggtcgag ggaggcgtgt tcaccggccg gccggcggcg
     4021 ttctgcttcg gccaccacaa ggtcgcgctc gccgagcgct gggccgaccg ccacgacatc
     4081 gacctcgcgg cgagctactt cgacagcgac agctacaacg ctctgccgat gctcgagcgg
     4141 gtcggcaccg cgatcgcggt caaccccgac gcgcggctgc gccgccacgc gcgccgccgc
     4201 ggctgggcga ccccgcgctg ggcgtgactc gacgtagact ccaccctcga cctcgacgtc
     4261 gacctcggag tcaaggtgca aggtcaaggg tggggtcaga cgactacgtt gccgtcgacg
     4321 tcaagggtcg ggtcaaggtc atcgtcgagg acaaggtcga acttcggaat cggcttccgt
     4381 cctcttcgac acctgttcag gcgccgtggt agccgagcgc gatcgccggg atgtcgagcg
     4441 cggcgaggat catcgtcggc gaggtcgcgc cgagcacgtc gccgatccgg gtgtagccgg
     4501 gatcggtgcc gggccggttg tcccaccgca ccggcgggtc gaggccgcgg aacaccgtca
     4561 cgctgaacca gccggtctgc gcgctgtaca tcacgtcgta caccggcccg ggcagctcgg
     4621 cggcgtagtc gatcaccagg tcaccgggcc ggccggtgac cagcgtgccg agcttgttcg
     4681 ctcgcagcgt ggtgcgcgcg ttggtcccga ccgccgcgac ttgctcgggg gctgcgccga
     4741 ccacgacgca ggccgtgcgc gcggcgggaa ggtcctcgga ggtcgccatg cccacaccat
     4801 ggccgcgaag cccgcacgct gcaacgctac agcaatcccc ccacggcgag cgtcgagacg
     4861 gctcgactcg cgtcgtcggt tgtgtatgct gcatccggca tgacgactcc tgtgctcggg
     4921 gggctggcgg cgctccccgc ggttcgccgg ttgatcgagc tcgcgctcga cgaggatctc
     4981 ggacgcggtg acgcgacctc gcgcgcggtg ttcggcggcg cggtgcccgg cgcggtgccg
     5041 gtcgcagtgc cccgggtcgt cgccgacatg gtggcgcgcg atccgatcgt cgcgttcggc
     5101 atcgaggtcg ccgccgcggt gttcgcccgg gtcgatccgg cgatcgcggt cgaggccggc
     5161 tgccccgacg ggtcgcacgt cgatcgcggc
//
LOCUS       DASVOR010002463         2168 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_28917,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002463 DASVOR010000000
VERSION     DASVOR010002463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28917"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1518
                     /locus_tag="VF516_45185"
     CDS             <1..1518
                     /locus_tag="VF516_45185"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8115029.1"
                     /translation="AYFAGPGLGPGLAPGVRGRRHRPLNPPLTAPDHFPTCSHLEIRE
                     IQRSHPRQSGRCPGHHLWSCETEGFWLMETRAKVLHWFIRGGTLTTPSGATHTIGVEP
                     IVVGRNEGADVVLDDHEVSGVHCELRAVTEGIAVRDLGSTNGTFCSNVRIRDALITSN
                     AELTVGGTRLTITPSGNKQRVELGYSDRFGSIVGVSPRMRRVFSVLEKVAATPLSVLV
                     LGETGTGKEGTARAIHDASPRKGGPFVVVDCGSIPQTLAESILFGHEKGAFTGANERR
                     LGALAEANGGTLFLDELGELPIDLQPKLLRALSERQVKRIGGTTFDHIDVRVIAATRR
                     DLSSEMNAGRFRSDLFFRIAQVRVELPPLRERLGDLPMLIEDICTRVQQAWAAPIVHD
                     WIGRRMASHDWPGNVRELVNVVSVAATLAHTPDAIDDVLLLARSGDQPTQSTQPATAF
                     SDAKRQAIEAFERNYFTELNRRAKGNVSEMARQSGMERHHVRAYLRKYGVDKPAG"
     gene            complement(1545..>2168)
                     /locus_tag="VF516_45190"
     CDS             complement(1545..>2168)
                     /locus_tag="VF516_45190"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEX8115030.1"
                     /translation="VTLEHMMVSYADRPGWFDACAGALQRALDLRTEFFDRLFDALQK
                     ARLEPLLARMQARRSLASELDDAALCHRPGGDTIAMYANEVEKLNIQFTVERLPVAAE
                     VLDPRMVRIPAGKFNEKHRHAHETLIHILEGSGQVLIDDRVLPVRAGDTVLVPRWAMH
                     QTQNLGSGELRFLAVTDFHLSQRAFLGDPTDYRMHVDVDGRRRHPPA"
BASE COUNT          319 a          780 c          760 g          309 t
ORIGIN      
        1 gcctacttcg ccgggcccgg gctcgggccc gggctcgccc ccggcgtccg cgggcgccgc
       61 caccggccgc tgaacccgcc gctgaccgct ccggaccact ttccgacgtg ctcccacctc
      121 gagatccggg agattcagcg cagccacccg cgtcaatccg ggcgatgccc tggacaccac
      181 ctctggtcat gcgagactga gggcttctgg ctcatggaaa cgcgtgccaa agtccttcac
      241 tggttcatcc gcggcggtac gttgaccacg ccgagcggcg ccacgcacac gatcggcgtc
      301 gagccgatcg tggtcgggcg caacgagggc gccgacgtgg tgctcgacga tcacgaggtc
      361 agcggcgtgc actgcgagct gcgtgcggtc accgagggca tcgccgtgcg cgacctcggc
      421 agcaccaacg gcacgttctg ctccaacgtg cggatccgcg acgcgctgat cacgtcgaac
      481 gccgagctca ccgtcggcgg cacccggctg acgatcacac cgtcgggcaa caagcagcgg
      541 gtcgagctcg gctactccga ccggttcggc tcgatcgtcg gcgtgtcgcc ccggatgcgc
      601 cgcgtgttct cggtgctcga gaaggtcgcc gcgaccccgc tcagcgtgct ggtgctcggc
      661 gagaccggca ccggcaagga gggcaccgcg cgcgcgatcc acgacgcgag cccgcgcaag
      721 ggcggaccgt tcgtggtcgt cgactgcggc tcgatcccgc agaccctggc cgagagcatc
      781 ctgttcggcc acgagaaggg ggcgttcacc ggcgccaacg agcgccggct cggcgcgctg
      841 gccgaggcca acggcggcac gctgttcctc gacgagctcg gcgagctgcc gatcgacctc
      901 cagcccaagc tgctgcgcgc gctgtccgag cgccaggtca agcggatcgg cggcaccacg
      961 ttcgatcaca tcgatgtccg ggtgatcgcc gcgacccggc gtgacctcag ctcggagatg
     1021 aacgccggcc ggttccgctc cgacctgttc ttccggatcg cgcaggtccg cgtcgagctg
     1081 ccgccgctgc gcgagcggct cggtgacctg ccgatgctga tcgaggacat ctgcacccgc
     1141 gtccagcagg cgtgggccgc gccgatcgtc cacgactgga tcgggcggcg gatggcgtcg
     1201 cacgactggc cgggcaacgt gcgcgagctg gtcaacgtgg tgtcggtcgc tgccaccttg
     1261 gcccacacgc ccgacgcgat cgacgacgtg ctgctgctgg cgcgcagcgg cgaccagccg
     1321 acccagtcga cccagcccgc gaccgcgttc tccgacgcca agcgccaggc gatcgaggcg
     1381 ttcgagcgca actacttcac cgagctcaac cggcgtgcca agggcaacgt cagcgagatg
     1441 gcgcggcaga gcgggatgga gcgccaccac gtgcgcgcct acctgcgcaa gtacggcgtc
     1501 gacaagccgg cgggctgacc ggcgcggacg ggcgctgcgc tacgctacgc cggcgggtgg
     1561 cggcgccggc cgtcgacgtc gacgtgcatg cggtagtcgg tcggatcgcc gaggaacgcg
     1621 cgctggctca ggtggaagtc ggtgaccgcg aggaagcgca gctcgccgct gcccaggttc
     1681 tgggtctggt gcatcgccca gcgcggcacc agcacggtgt cgccggcacg caccgggagc
     1741 acgcggtcgt cgatcagcac ctggccgctg ccctccagga tgtggatcag ggtctcgtgt
     1801 gcgtgcctgt gcttctcgtt gaacttgccg gcggggatcc gcaccatgcg cggatcgagc
     1861 acctcggcgg cgaccggcag gcgctcgacc gtgaactgga tgttcagctt ctcgacctcg
     1921 ttggcgtaca tcgcgatcgt gtcgccgccg ggccggtggc agagcgccgc gtcgtcgagc
     1981 tcgctcgcca gcgaccggcg cgcctgcatg cgcgccagca gcggttccag ccgggctttc
     2041 tgcagcgcgt cgaacaaccg gtcgaagaac tcggtgcgca gatcgagcgc ccgctgcagc
     2101 gcgccggcgc aggcgtcgaa ccaccccggc cggtcggcgt acgagaccat catgtgttcg
     2161 agcgtcac
//
LOCUS       DASVOR010002464         3044 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_465371,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002464 DASVOR010000000
VERSION     DASVOR010002464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465371"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VF516_45195"
     CDS             complement(<1..873)
                     /locus_tag="VF516_45195"
                     /inference="COORDINATES: protein motif:HMM:NF012876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HEX8115031.1"
                     /translation="MRSSLRNRLLITFLALVVIVGTGTLFAIERTLAGDLTASLDSRM
                     TMQAREAAGWLSNAGHLDRLAPRLAAAMGTRITIVDAEGLVLGDSHEPEAVGRPVGDA
                     WEVSRARQGEVGHAIRTLKSDEPPQYLVAVPADEGMVIRLAVPLGDLVLTRARMRNRL
                     LVGFGFGFLGCLVLSFIFIRALTRPLQQMTHTAEKLARGDYEAPPPPIDSGGELGVLA
                     RAMDRMAGEVKSRVGELTEQRDLLSVVFGGLVEGVIVVGRDRRIALVNDAARPLIGGG
                     SELPDRLAPLVTRAL"
     gene            complement(905..1618)
                     /locus_tag="VF516_45200"
     CDS             complement(905..1618)
                     /locus_tag="VF516_45200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8115032.1"
                     /translation="MERRVVIIEDERDVARLLEFNLRGAGFTVASAATGAAGLDEVRR
                     TRPDVVVLDLMLPDQSGYEVCKQIRADPVVGDAGVLMLTARGEAEDRILGLEVGADDY
                     VVKPFVVREVVLRVTALANRLAERRTRPAASEPGGTRKVGPIELDPVTHDVRIDGAPA
                     QLRPLEYKLLQLLVSDPGRVFSREELLEQVWEMRGDINTRTVDVHVRRLRVSLGPAAD
                     VIETVHGFGYRARRDGGPG"
     gene            1648..2799
                     /locus_tag="VF516_45205"
     CDS             1648..2799
                     /locus_tag="VF516_45205"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEX8115033.1"
                     /translation="MVVVSGAAQPMKPETAAAQALHSIDDATGAVVPAIHLATTYARD
                     ADYASRGQTYSRDENPTPKAAERVLAALEGGAAALTFASGMAAASAVFRGLCRPGDHV
                     ISPRVGYYTLRAWLEKFCARWGLAHDLVDTTDLAAVRAALRPTTRVVWLETPANPTWE
                     VSDIAAVAELAHAAGAVVAVDSTCATPVHTQPIALGADLVMHSATKYLSGHSDVLAGA
                     LVTARADAAWAQIVELRHDEGPLLGPLEAYLLTRGMRTLFARVERSSRTAQRIADELA
                     ARGVTVRYPGLASHPQHTIAARQMQRGFGSMLSIQVGGGAERALVVLRRLRVWIPATS
                     LGGVESLVEHRHTVEGPITTAPPDLLRLSVGLEHEADLLDDLVATLAAP"
     gene            2805..>3044
                     /locus_tag="VF516_45210"
     CDS             2805..>3044
                     /locus_tag="VF516_45210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115034.1"
                     /translation="MAAALDLRIATCVALPEPDVDQAPLSAALAAAGLSAALVGWDDP
                     AADWDGYDVVLVRSVWDADQRRDEFVAWARRVAAGP"
BASE COUNT          395 a         1204 c         1070 g          375 t
ORIGIN      
        1 cagcgcgcgg gtcaccagcg gcgccagccg gtcgggcagc tcgctgccac cgccgatcag
       61 cggccgggcg gcgtcgttga ccagcgcgat ccggcgatcg cggccgacca cgatcacgcc
      121 ctcgaccagg ccgccgaaca ccaccgacag gaggtcgcgc tgctcggtca gctcgccgac
      181 ccgcgacttg acctcgccgg ccatccggtc catcgcgcgc gccagcacgc cgagctcgcc
      241 gcccgagtcg atcggcggcg gcggcgcctc gtagtcgccg cgcgccagct tctcggcggt
      301 gtgggtcatc tgctgcagcg gccgggtcag cgcgcggatg aagatgaacg acagcaccag
      361 gcagccgagg aagccgaacc cgaagccgac cagcagccgg ttgcgcatcc gcgcgcgggt
      421 gagcaccaga tcgccgagcg ggaccgcgag ccggatcacc atcccctcgt ccgccgggac
      481 cgcgaccagg tactgcggcg gctcgtcgct cttcagcgtg cggatcgcgt ggccgacctc
      541 gccttggcgc gcccgagaca cctcccaggc atcgccgacc ggccggccga ccgcctcggg
      601 ctcgtgcgag tcgcccagca ccaggccctc ggcgtcgacg atcgtgatcc gcgttcccat
      661 cgccgccgcg agccgcggcg cgagccggtc gaggtggccg gcgttcgaca gccagcccgc
      721 cgcctcgcgc gcctgcatcg tcatccggct gtcgagcgac gcggtcagat cgccggccag
      781 cgtgcgctcg atcgcgaaca gcgtcccggt gccgacgatg acgaccaggg cgaggaacgt
      841 gatcagcagg cggttgcgga gcgagctgcg cacgccgtgc cgagcgtacc agcggggatc
      901 ccgatcagcc aggaccgccg tcgcgccgcg cgcggtagcc gaagccgtgc acggtctcga
      961 tcacatcggc ggcggggccc aggctgaccc gcagccggcg gacgtggacg tcgaccgtgc
     1021 gggtgttgat gtcgccgcgc atctcccaga cctgctcgag cagctcctcg cgcgagaaca
     1081 cccggccggg gtcgctgacc aggagctgca agagcttgta ctcgagcggc cgcagctggg
     1141 cgggcgcgcc gtcgatccgg acgtcgtggg tcaccggatc gagctcgatc gggccgacct
     1201 tgcgcgtgcc gccgggctcg ctcgccgccg gccgggtccg gcgctcggcc agccggttgg
     1261 ccagcgccgt gacccgcagc acgacctcgc ggaccacgaa cggcttgacc acgtagtcgt
     1321 cggccccgac ctcgagcccg aggatgcggt cctcggcctc gcccctggcg gtgagcatca
     1381 gcacgcccgc gtcaccgacc accggatcgg cgcggatctg cttgcacacc tcgtagcccg
     1441 actggtcggg cagcatcaga tcgagcacga cgacatcggg ccgggtgcgg cgcacctcat
     1501 cgaggccagc cgcgccggtc gccgccgacg ccaccgtgaa cccggcgccc cgcaggttga
     1561 actccagcag gcgggccacg tcccgctcgt cctcgatgat gaccacgcgg cgttccaccc
     1621 gccggttata gcttggttac accggcgatg gtggtagtgt ccggcgccgc ccagcccatg
     1681 aagccagaga ccgccgccgc ccaggccctg cattccatcg acgacgcgac cggcgcggtg
     1741 gtcccggcga ttcacctcgc gacgacctac gcgcgcgacg ccgactacgc gtcgcgcggc
     1801 cagacctact cgcgcgacga gaacccgacg ccgaaggccg ccgagcgcgt gctcgcggcg
     1861 ctcgagggcg gcgccgccgc gctgaccttc gcgtcgggga tggccgcggc gagcgcggtg
     1921 ttccgcgggc tgtgccggcc cggcgatcac gtgatcagcc cgcgggtcgg ctactacacc
     1981 ctgcgcgcct ggctcgagaa gttctgcgcg cgctggggcc tggcccacga tctggtcgac
     2041 accaccgacc tcgcggcggt gcgcgccgcg ctgcgcccga ccacgcgcgt ggtgtggctc
     2101 gagaccccgg ccaacccgac ctgggaggtc agcgacatcg ccgcggtcgc cgagctcgcg
     2161 cacgccgccg gcgccgtggt cgcggtcgac tcgacctgcg cgaccccggt ccacacccag
     2221 ccgatcgcgc tcggcgccga cctcgtgatg cactcggcga ccaagtacct gtccggccac
     2281 tccgacgtgc tcgccggcgc gctggtcacc gcgcgggccg acgccgcgtg ggcccagatc
     2341 gtcgagctcc gccacgacga gggcccgctg ctgggcccgc tcgaggccta cctcctgacc
     2401 cgcggcatgc gcacgctgtt cgcgcgggtc gagcggtcga gccgcaccgc gcagcggatc
     2461 gccgacgagc tcgcggcgcg cggcgtcacc gtgcgctacc ccggcctggc gtcgcacccg
     2521 cagcacacga tcgccgcccg ccagatgcag cgcgggttcg gcagcatgct ctcgatccag
     2581 gtcggcggcg gcgccgagcg agcgctcgtg gtcctccggc ggctgcgggt ctggatcccc
     2641 gcgacctcgc tgggcggcgt cgagagcctg gtcgagcacc gccacaccgt cgaggggccg
     2701 atcaccaccg ccccgccgga cctgctgcgc ctctcggtcg gcctggagca cgaggccgac
     2761 ctgctcgacg acctggtcgc cacgctggcc gcgccgtaag ccggatggcc gccgcgctcg
     2821 acctgcggat cgcgacctgc gtcgcgctgc ccgagcccga cgtcgaccag gcgccgctgt
     2881 ccgccgcgct cgccgccgcc ggcctgtccg ccgcgctcgt cggctgggac gacccggcgg
     2941 ccgactggga cggctacgac gtggtcctcg tccgctcggt ctgggacgcc gaccagcgcc
     3001 gcgacgagtt cgtcgcctgg gcccgtcgcg tcgccgccgg cccg
//
LOCUS       DASVOR010002465         6149 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_523562,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002465 DASVOR010000000
VERSION     DASVOR010002465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6149
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523562"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(368..1063)
                     /locus_tag="VF516_45215"
     CDS             complement(368..1063)
                     /locus_tag="VF516_45215"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEX8115035.1"
                     /translation="MFEDIEYEVADRTFRGVLAAPSTVAKRAGVLVFHGGSGPTEHER
                     QVVRRLAALGYTALAPDLFGEVFPDRAHGMHVIGQLVADPERLRQRSTAALAYLRTRG
                     VQHAFAIGHCFGGLAALELARSGAAIEAAVSFHGALTTRAPARASEVRARVLVCTGAD
                     DPFCPPDHRAAFEAEMTAAGVDWQHHIYASARHGFTVRDIDPAKHPGCAYHELAANRA
                     WAAMIALFAEVPG"
     gene            complement(1171..1971)
                     /locus_tag="VF516_45220"
     CDS             complement(1171..1971)
                     /locus_tag="VF516_45220"
                     /inference="COORDINATES: protein motif:HMM:NF004781.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEX8115036.1"
                     /translation="MNPVTTDRILVHAADGIGWLTLNNPERRNAISLEMWRGIGAALE
                     AFQHDDAVRVAVIRGAGGTAFASGADISEFDQHRANAEQRKEYTAAGALGHEWLRRFD
                     KPLLALIEGFCIGGGLVVALCADVRFATPASRFAIPAAKLGLGYEYSGVAALARLIGP
                     SATRDLLFSARLVEADEALRLGLINFVIAGDEIEAKVRSYAQAIAANAPLTIRAAKAA
                     VAAFEHPPLTEATEAHLRALVDACFDSEDYAEGRRAFAEKRPPKFRGR"
     gene            complement(2037..2669)
                     /locus_tag="VF516_45225"
     CDS             complement(2037..2669)
                     /locus_tag="VF516_45225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115037.1"
                     /translation="MTVRPPWYEPSRIAQADRLPHQVRVGPGEIAAAYAMGTADGFEP
                     LSAAIERMRRRVGELVCGGPIAEPHFANAWERVRKLYPCCSPPCAARFDPDIQWLPAE
                     RPPPATPDDEQRLVRTGAERLRAGDRPSLVVRDLLLAGIAPRAVRKLLTDAELSVAAN
                     DRWVDRLNVLGVISLLFGGRWRVAERNDRRDLEQLRTAATDLETWSARFG"
     gene            complement(2941..3942)
                     /locus_tag="VF516_45230"
     CDS             complement(2941..3942)
                     /locus_tag="VF516_45230"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115038.1"
                     /translation="MRTKTMRWLVIAAIGCAHASQGRPMTTEIKVTLDKLAELDGGGA
                     RLAVHGALIAAATDDAITVWRDQIRVAAASSPDPIDRVRLSADGKQLLAAPYVFDVAG
                     ASWVALDDIEPALLHGLAGPSKGELKVRRADWSADGQELLVYVEHRAARGVASGSSSG
                     AQARLLWMDRARKVIAVPWTGRNYALTSVGFGAHWAAAGGKQLMVWDRKTHAQVAALD
                     VHSLVIREIVFSPDDKLVASVSNDHKVVLVDTASWKMIANWDAHDSDAVGVAWHPSKP
                     LIATTGQDALVRLWSLDGKRVAEARLPRAGNAVAFSDDGSKLVVATDDKVIVFALKP"
     gene            complement(3939..4436)
                     /locus_tag="VF516_45235"
     CDS             complement(3939..4436)
                     /locus_tag="VF516_45235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115039.1"
                     /translation="MLDGLLPVPPPATPPVGALTLALIDVAQRRDKASVRAISYLQGV
                     LGVERWFPARLGVTARLDGLATENEILIAIDRAKPSAKTPDPNIDTDRDGKNDAYVEP
                     MTKTAKVDVPTADIYEKADKTSKKLGSLAKGADVRIVGKASVAWLAIEYNGGTAFAQV
                     IDIAV"
     gene            complement(4838..5209)
                     /locus_tag="VF516_45240"
     CDS             complement(4838..5209)
                     /locus_tag="VF516_45240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115040.1"
                     /translation="MCLPLSREGSNVVGSVAALATREPHSGPNDGLRPAAGPWSPRCL
                     HIADARVYEDLVEVEALAVTADEAVTNLPPKPLGKYSRTMARLYTLVSKTSGRAQDAL
                     ERGEELVAQIAAARGGKRRAR"
     gene            complement(5423..5737)
                     /locus_tag="VF516_45245"
     CDS             complement(5423..5737)
                     /locus_tag="VF516_45245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115041.1"
                     /translation="MSDLTAKEQKAVRTALRFLRLRVGAWKPLVKALRYEYDSIQKVA
                     SGRKAVTPALALRVARFVGVPMDELLAGQWLSPRVCPHCGHPPDDFQDEDTIVRDEQI
                     PG"
     gene            complement(5734..>6149)
                     /locus_tag="VF516_45250"
     CDS             complement(5734..>6149)
                     /locus_tag="VF516_45250"
                     /inference="COORDINATES: protein motif:HMM:NF013752.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEX8115042.1"
                     /translation="DVREVSADKAYSSVDNHNVMESLNVEAFVPFKVNAVVNPKAPVW
                     SRHLVEFTLNQERFLRHYHRRSNVETVFSMLKARFGASVSSRLPTARANEVLAMCVAH
                     NLCCLVKAIFTAGLAPAFWQDAPAALLSLVPEEQA"
BASE COUNT          915 a         2179 c         2068 g          987 t
ORIGIN      
        1 gatcaaccgg aacctcaagt gaccgcgctc cgcgcgttca cggtccaggg tcagcgctgt
       61 cgtttcaccg aacgatcgca gtgactgccc cggatcccgc tcgaccccgg cgctaggcga
      121 gcacctcggc gaacagaccg agcatcgcgc gtgccaggat gtgacgcgcg tgagagcggg
      181 acgcgagcgc cggagggctc ccgctgcgct ccaccggcgc cggcgacggg ccgcgagcgc
      241 gtgcccgacc gggcgccggt gcgaggtgcg cgacgacccg agcgccggtg tgcgcgcacg
      301 gtgagcacgc ccgcgtcgag cggtgcgcac ccatcagcgc gattctgtcg gacgcaacgc
      361 cgcgctgcta accaggcacc tcggcgaaca gcgcgatcat cgcagcccac gctcggtttg
      421 ccgcgagctc gtgatacgcg cagccggggt gcttcgcggg atcgatgtcc cgcacggtga
      481 agccgtgccg cgcgctcgcg tagatgtggt gctgccagtc gacgccggcg gcggtcatct
      541 ccgcttcgaa cgcggcgcgg tggtcgggtg ggcagaacgg atcgtccgcg cccgtgcaga
      601 ccagcacgcg cgcacgcacc tcgctcgccc gcgccggggc ccgggtcgtc agcgcaccgt
      661 gaaaactcac cgcggcctcg atcgccgctc cgctgcgggc gagctcgagc gccgccagtc
      721 cgccgaagca atggccgatc gcgaacgcgt gctggacacc tcgggtgcgc aggtaggcga
      781 gcgccgcggt cgagcgctgc cgcagccgct ccggatcggc cacgagctgg ccgatcacgt
      841 gcatgccgtg tgcgcgatcg gggaagactt ccccgaacag atccggggcc agcgcggtgt
      901 agcccagggc ggcgagcctg cgcacgacct gccgctcgtg ctcggtcgga cccgagccgc
      961 cgtgaaacac cagaacaccc gcccgcttcg cgacggtgct cggagctgcc aggacgccac
     1021 ggaacgtccg atcggcgacc tcgtactcga tgtcttcgaa catgcgctca tccttgtgaa
     1081 gatttgcgca gatctaatta tcaccatttt cgggaatttg tcaatgtcca aggatcgtgc
     1141 cgtggcggct cgatgccatg ccgcgccgtg ctatcgaccg cggaacttcg gcggccgctt
     1201 ctcggcgaag gcccggcgac cctcggcata gtcctcactg tcgaagcacg catcgacgag
     1261 tgcgcgcagg tgggcctcgg tggcctcggt cagcggcggg tgctcgaacg cggcgaccgc
     1321 ggccttggcg gcgcgaatcg tcagcggcgc gttcgctgcg atcgcctggg cgtagctgcg
     1381 taccttggcc tcgatctcgt cgccggcgat gacgaaattg atcagcccga gccgcagcgc
     1441 ttcgtcggct tcgaccagcc gtgcgctgaa caagaggtcg cgcgtggccg acggtccgat
     1501 cagccgggcc agcgctgcca cgccgctgta ctcgtagccg agcccgagct tggccgccgg
     1561 gatcgcaaag cgcgacgccg gggtcgcaaa ccgcacgtca gcacagagcg cgaccacgag
     1621 cccaccgccg atgcagaacc cttcgatcag cgcgagcagg ggcttgtcga accgacgcag
     1681 ccattcatga ccgagcgcgc cggcggccgt gtactccttg cgctgctcgg cattggcgcg
     1741 gtgctgatcg aactccgaga tgtccgcgcc cgacgcgaac gccgtgccac ctgcgccgcg
     1801 gatcaccgcc acgcgaacgg catcgtcgtg ctggaacgcc tcgagcgcgg cgccgatgcc
     1861 acgccacatc tcgagcgaga tcgcgttgcg gcgctccggg ttgttgagcg tcagccagcc
     1921 gattccgtcg gcggcatgca ccaggatccg atcggtcgtg acagggttca tgcgatggct
     1981 cctttgcgat gtagctcgtc gatggacgcc cggttcggtg cggctcgagc gcgacactaa
     2041 ccgaaccggg cgctccaggt ctcgaggtcg gtcgcggccg tgcgcagctg ctcgagatct
     2101 cgacgatcgt ttcgctcggc gacgcgccag cggccgccga acagcaggct gatcacaccg
     2161 agcacgttga ggcgatcgac ccagcggtcg ttggcggcga ccgacagctc tgcgtcggtc
     2221 aagagcttgc gcaccgccct cggtgcgatc ccggcgagca gcaggtcgcg gacgacgagc
     2281 gacgggcggt cgcctgcccg caggcgctcg gcgccagtgc gtacgagccg ttgctcatcg
     2341 tccggcgtcg ccggcggcgg tcgctccgcg ggcagccact ggatgtccgg atcgaaccgg
     2401 gccgcgcatg gcgggctgca gcacggatag agcttgcgca cgcgctccca cgcgttcgcg
     2461 aagtgcggct ctgcaatcgg tcccccgcag acgagctcac cgacccgccg gcgcatccgc
     2521 tcgatggcgg cggacagcgg ttcgaagccg tccgcggtcc ccatcgcgta ggcggccgcg
     2581 atctcgccgg gaccgacgcg cacctggtgc ggcagccggt cagcctgcgc gatcctggac
     2641 ggctcgtacc acggcggtct gaccgtcacg ttccagccga ggcagaacac tgcctcgccc
     2701 tggtggaggg cctcgtttcg ccaagctact gtcggttggg cgatgctgct cgctccctgg
     2761 aaggatcaag gccgcccgcg ccttggtgtc gctggtcagg atcctccgcg agatattcgg
     2821 cctcaagccg gtcccggaca cgcaggcgga gcagcccggt cgcctcgcgc atgcagatcg
     2881 ggatcgatgc agcggtggta ttgccgttcc gcgagttggc ggtgacgacc ggcgcggcgg
     2941 ctacggcttg agcgcgaaca cgatgacctt gtcgtcggtc gcaaccacga gcttgctgcc
     3001 atcgtcgctg aacgccacgg cattgccggc gcgcggcagc ctggcctcgg cgacgcgctt
     3061 accgtcgagc gaccacagcc ggaccagcgc gtcctggccg gtggtagcga tcagcggctt
     3121 cgacgggtgc cacgccacgc cgaccgcgtc gctgtcgtgg gcgtcccagt tcgcgatcat
     3181 cttccagctc gcagtgtcga ccagcaccac cttgtgatcg ttgctcacgc tggcgaccag
     3241 cttgtcgtcc gggctgaaca cgatctcccg gatcaccagc gagtggacgt cgagcgcggc
     3301 gacctgggcg tgggtcttgc ggtcccacac catgagctgc ttgccaccag cggcggccca
     3361 gtgcgcgccg aacccgacgc tggtcagcgc gtagttcctg ccggtccatg gcaccgcgat
     3421 gaccttgcgc gcgcggtcca tccacagaag gcgcgcctgc gcgccgctcg agctacccga
     3481 cgcgacccca cgtgccgcgc ggtgctcgac gtagaccagc agctcctggc cgtcggcgct
     3541 ccagtcggcg cgccggacct tgagctcgcc cttcgaaggt ccggccaggc cgtgcagcag
     3601 ggcgggctcg atgtcgtcga gtgcgaccca gctcgcgccc gccacatcga acacgtacgg
     3661 cgcagccagc agctgcttgc cgtcggcgct cagccgcacg cggtcgatcg ggtcgggtga
     3721 gcttgccgcc gcgacgcgga tctgatcgcg ccacaccgtg atcgcgtcat cggtcgcggc
     3781 tgcgatcagc gcgccgtgga cggcgagccg cgctcctccg ccgtcgagct ctgccagctt
     3841 atcgagcgtc accttgatct ccgtggtcat cgggcgtccc tggctcgcgt gcgcgcaacc
     3901 gatcgccgcg atcaccagcc agcgcatcgt tttcgtcctc atacggcaat atcgatcacc
     3961 tgcgcaaatg cggtaccgcc gttgtactcg atcgcgagcc aggcgacaga cgccttgccg
     4021 acgattcgca cgtcggcgcc cttggccagg ctgcccagct tcttcgacgt cttgtcggcc
     4081 ttctcgtaga tatccgcggt cggcacgtcg accttggcgg tcttggtcat cggctcgacg
     4141 tacgcgtcgt tcttgccgtc gcggtcggtg tcgatgttgg gatccggggt cttggccgac
     4201 ggcttggcgc ggtcgatcgc gatcaggatc tcgttctcgg tcgccagacc gtcgagcctg
     4261 gcggtcacgc ccagccgcgc cgggaaccag cgctccacgc cgagcacgcc ctgcaggtag
     4321 ctgatcgcgc ggacgctcgc cttgtcgcgg cgctgcgcga cgtcgatcag cgccaaggtc
     4381 agcgcgccga ccggcggtgt cgccggcggc ggcaccggca acaggccgtc gagcaccccg
     4441 gcgccgatca ggaagtcgag acatcgtgag tcggaccaaa cgcagcctgg atcttctcgc
     4501 ggtggggcag cgcttctgtc gcaccttgtg tgcctcgggc ggcgacgtcg ccgggcgctg
     4561 gtcctggagc cactccgggc tgcgactgct tcagctggac gctctcgacg gcagtccgct
     4621 tgccgaccga tccctggtcg cgcagcccac caccgcccgt tgttctggat tcgttgctgc
     4681 gcgtctgccc acggaagctc atcgtcgccc cacctgctta cgagttgata cgcaacgagc
     4741 cgcgaaaagc tacctgcctc cgctgacctg cgacccacgc tccacgacct cgcgtggcgg
     4801 tacttcgcga cctacccgcc caacgccacg ccgtagctca gcgcgcccgg cgcttgccgc
     4861 cgcgggctgc cgcgatctgc gccaccagct cctcgccccg ctccagcgca tcctgtgcgc
     4921 gcccggaggt cttgctgacc agtgtgtaca gccgggccat ggtgcggctg tacttgccca
     4981 atggtttcgg cggcaggttc gtcacggcct cgtcggcggt gaccgcgagg gcctcgacct
     5041 cgaccaggtc ttcgtagacg cgggcgtctg cgatgtgaag gcatcgtggg ctccaaggcc
     5101 cggcggcggg tcgcaacccg tcgttcgggc cgctatgggg ttcacgtgtc gccaacgcgg
     5161 cgacactgcc cacgacgtta ctgccttccc gtgacagcgg gagacacacg gcgcgccact
     5221 caatcctcga cggagagcgg gcgatcggcg gattaacctg cagccgaatc aaaaacctgc
     5281 aggagtcagt tttcgacgag aaacctgcaa agcctccgtt gtccgctgtc cgctgtccgc
     5341 tgcccgctgt ccgctgtcgg ctttgcacct ttggagcgtt cctcgtcgaa aaaaggtgca
     5401 gccgctccga ataaaggtgc agctaccccg ggatctgctc gtcgcggacg atcgtgtcct
     5461 cgtcctggaa atcgtccggc gggtggccgc agtgcgggca gacgcggggc gacaaccact
     5521 ggccggcgag tagctcgtcc atcggcacgc cgacgaaccg cgccacgcgc agcgccagcg
     5581 cgggcgtgac ggccttcctg ccgctcgcca ccttctgaat gctgtcgtac tcgtagcgca
     5641 gcgccttgac cagcggcttc cacgcgccga cgcgcagccg caggaaccgc agcgcggtgc
     5701 ggacggcctt ctgttccttc gcggtcaggt cactcatgct tgctcctcgg gaaccaggga
     5761 aagcagggcg gcgggcgcgt cctgccagaa cgcgggcgcg agcccggcgg tgaagatggc
     5821 cttgaccagg cagcacaggt tgtgcgccac gcacatcgcg aggacctcgt ttgcgcgagc
     5881 ggtcggcagt cgcgacgaaa cggaggcccc gaaccgtgcc ttgagcatcg agaagaccgt
     5941 ctcgacgttc gaccggcgat ggtagtgccg gaggaaccgc tcttgattga gcgtgaactc
     6001 gacgaggtgg cgcgaccaca ccggggcctt ggggttgacc accgcgttga ccttgaacgg
     6061 aacgaacgcc tcgacgttga gcgactccat cacgttgtgg ttgtcgaccg acgagtacgc
     6121 cttgtcggcg ctgacctcgc gcacgtcgt
//
LOCUS       DASVOR010002466         2681 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_115996,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002466 DASVOR010000000
VERSION     DASVOR010002466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115996"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            294..1523
                     /locus_tag="VF516_45255"
     CDS             294..1523
                     /locus_tag="VF516_45255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8115043.1"
                     /translation="MVAPPTTPRSFAVDVARASAAFQELARQMGAQFLDKQEIIRLMT
                     VSAIAGEHMVIVGPPGTAKSAMIDMFAKLIDARYFEYLLTRFTEPNELFGPVDISAFR
                     EGRYTRRLENMLPTAEIVFLDEIFKSNSAILNALLHVINERKFQNGPEVVQVPLISLY
                     AASNEVPNDDNLAAMFDRFLVRVLSDNLDSYHFHELMKKGVSLELRKMTGRGLDAQGR
                     PTGELRPVISARELRHIQQNFDKFMAFPEEFLTKYKGLVFQIRSEGISVSDRRAVKLL
                     KLFAASAVFDGRTRVHDGDFFILRHIWNNLDQVELLEEIVNPVVDGYYREHPDERRFI
                     GPSASLDDLLTELALIRDLLTSGSELSDIQLFSQLKNLNEIKVALAALPGDAAVRMIR
                     EVDQLLETVFASSKFGL"
     gene            complement(1545..2447)
                     /locus_tag="VF516_45260"
     CDS             complement(1545..2447)
                     /locus_tag="VF516_45260"
                     /inference="COORDINATES: protein motif:HMM:NF004843.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8115044.1"
                     /translation="MKTFQGRTAVITGAASGFGLEVARIAAAKGMAIVMADIQQDALD
                     AAVAEITALGAQVLPYRLDVSSAAAVEAMGAATQARFGAPHFVFNNAGVGSGGLVWEL
                     GVRDWEWVLGVNLMGVVHGVRVFTPMMLAAARRDPDYEGHIVNTASMAGLLNPPNMGV
                     YNVSKHAVVSLSETLYHDLGLVTDQVTASVLCPYFVPTGITRSERNRPDDLKDGKPTR
                     SQLVARAMSDKAVSSGKVTAATVAQFVFDALAERRFYIYSHPHALGNVQTRMEDIIAG
                     KNPSDPFAQRPEVGDALRKALRED"
     gene            complement(2456..>2681)
                     /locus_tag="VF516_45265"
     CDS             complement(2456..>2681)
                     /locus_tag="VF516_45265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115045.1"
                     /translation="ARAGGHAPAAVEPGLGFEAGAPVTVAATDYGTDPVAGTLVGLSA
                     GEVVIERRDERAGTVHVHFPRIGFQIRKAS"
BASE COUNT          408 a          950 c          902 g          421 t
ORIGIN      
        1 agcagggaac gcttcatgga aatctcctta gggaacaacg actcagctac tgcatggcgc
       61 gcgccgacgc acgggcggct atgtgtgtga atccgggtga cccgccgcgt gtcacgatgc
      121 cgcggcgggg cgatctgacc ccccaccgcc gcgggccgca caccggcagc ctccgggcgc
      181 tgcccgcgcg caagcagcgg atttctcagg ctggatcggc cgatcgaccg gccaccattc
      241 cggcggtcgg gcagctacgc gctcgacctt cctgaggtaa tgtggggccg ccgatggtcg
      301 cgccgcccac cactccgcga agcttcgccg tcgacgtcgc ccgggcatcg gcggcgttcc
      361 aggagctcgc gcgccagatg ggggcgcagt tcctcgacaa gcaggagatc atccgtttga
      421 tgacggtgtc ggcgatcgcc ggcgagcaca tggtgatcgt cggcccgccc ggcaccgcca
      481 agtccgcgat gatcgacatg ttcgccaagc tgatcgacgc gcggtacttc gagtacctgc
      541 tgacccggtt caccgagccc aacgagctgt tcgggccggt cgacatctcg gcgttccgcg
      601 agggccgcta cacgcggcgg ctcgagaaca tgctgccgac cgccgagatc gtgttcctcg
      661 acgagatctt caagtcgaac tcggcgatct tgaacgcgct gctccacgtc atcaacgagc
      721 gcaagttcca gaacggcccc gaggtcgtcc aggtcccgct gatctcgctg tacgcggcgt
      781 ccaacgaggt ccccaacgac gacaacctcg cggcgatgtt cgaccgcttc ctggtccgcg
      841 tgctgtcgga caacctcgac agctaccact tccacgagct gatgaagaag ggcgtgtcgc
      901 tcgagctccg caagatgacc gggcggggcc tcgacgccca gggccggccg accggcgagc
      961 tgcgcccggt gatctcggcg cgcgagctcc gccacatcca gcagaacttc gacaagttca
     1021 tggcgttccc cgaggagttc ctgaccaagt acaaggggct cgtgttccag atccggtccg
     1081 aggggatctc ggtgtccgac cgccgggcgg tcaagctgct caagctgttc gcggcctcgg
     1141 cggtgttcga cggccggacc cgggtgcacg acggcgactt cttcatcctg cgccatatct
     1201 ggaacaacct cgatcaggtc gagctgctcg aggagatcgt caacccggtg gtcgacggtt
     1261 actaccgcga gcaccccgac gagcgccggt tcatcggccc gtcggccagc ctcgacgacc
     1321 tgctcaccga gctcgcgctg atccgcgacc tgttgacctc gggcagcgag ctgtccgaca
     1381 tccagctgtt cagccagctc aagaacctca acgagatcaa ggtcgcgctg gccgcgctgc
     1441 cgggcgacgc cgccgtgcgg atgatccgcg aggtcgacca gctgctcgag accgtgttcg
     1501 cgtcgtccaa gttcgggctg tgaccgcggc ggcttcccgg gcgctcagtc ctcgcgcagc
     1561 gccttccgga gcgcatcgcc gacctcgggc cgctgggcga acggatcgct cgggttcttg
     1621 ccggcgatga tgtcctccat ccgggtctgc acgttgccca gcgcgtgcgg gtggctgtag
     1681 atgtagaacc ggcgctcggc gagcgcgtcg aacacgaact gcgcgacggt cgccgcggtc
     1741 accttgccgc tcgacaccgc cttgtcgctc atcgcgcgcg cgaccagctg gctcctggtc
     1801 ggctttccgt ccttgaggtc gtcggggcgg ttgcgctcgc tgcgcgtgat gccggtcggc
     1861 acgaagtacg gacacagcac cgacgcggtg acctgatcgg tgaccaggcc gaggtcgtgg
     1921 tacagcgtct cgctgagcga caccaccgcg tgcttgctga cgttgtagac gcccatgttg
     1981 ggcgggttga gcaggccggc catcgacgcg gtgttgacga tgtggccctc gtagtcggga
     2041 tcgcgccggg cggcggcgag catcatcggc gtgaacacgc ggaccccgtg cacgacgccc
     2101 atcaggttga cgccgagcac ccactcccag tcgcgcacgc cgagctccca gaccagcccg
     2161 ccgctgccga ccccggcgtt gttgaacacg aagtgcggcg cgccgaaccg cgcctgggtc
     2221 gcggcgccca tcgcctcgac cgcggcggcg ctcgacacgt cgagccggta cggcaggacc
     2281 tgcgcgccca gcgcggtgat ctcggcgacc gcggcgtcga gcgcgtcctg ctggatgtcg
     2341 gccatcacga tggccatgcc cttggccgcg gcgatccgcg cgacctccag cccgaaaccg
     2401 gaggcggcgc cggtgatcac cgcggtgcga ccctggaacg tcttcatggc agacctcagc
     2461 tcgccttcct gatctggaag ccgatgcgcg ggaagtggac gtggacggtg ccggcgcgct
     2521 cgtcgcgccg ctcgatgacg acctcgccgg ccgacagccc gaccagcgtg ccggcgaccg
     2581 gatcggtgcc gtagtcggtg gccgccaccg tgaccggcgc accggcctcg aagccgagcc
     2641 cgggctcgac cgcggcgggc gcgtggccgc cggcgcgggc c
//
LOCUS       DASVOR010002467         7371 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_261623,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002467 DASVOR010000000
VERSION     DASVOR010002467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7371
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_261623"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VF516_45270"
     CDS             complement(<1..870)
                     /locus_tag="VF516_45270"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEX8115046.1"
                     /translation="MSKKSRRWIILALVAAAIAAFFGVRYWKAKQTALPSGIAAGNGR
                     IESKQVDVASKLPLKVKEVFVAEGDMVKPGQVLVQMDTVTLDSQLAEARQAVAAAREQ
                     MAVAQANIVRRQSEIELARIEKERARKLLAEHAGSQRDYDVRTMTLKTSQAGRAEAQA
                     QLQVAQQQVKVGQANVATIQSRITDATLTSPVLGRVLYRLAEPGEVLAAGGKALTVVD
                     LGDVYMEIFLPSEQAAALKIGADARFTVDYEPNRVAPGYVSFVSPEAQFTPKEVETRS
                     EREKLMFRVKIQVP"
     gene            complement(877..2520)
                     /locus_tag="VF516_45275"
     CDS             complement(877..2520)
                     /locus_tag="VF516_45275"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEX8115047.1"
                     /translation="MKRSRVSRSDQETDVRDHLVSTLLVIGLVLPAPACLVGPDFVRP
                     KVALNTSWSESHDPRLAVNAATELAWWKTLDDPTLDELIELAYHQNLSLQVAGLRILE
                     ARALVGIAVGQQYPTNPGPIAHAGVVKPSEHSFGGFINHHYGEYQIGFDAIWELDFWG
                     KYRRGVRAANAAYLATVADYDDALVSLSAEVARTYVVIRTFEVLIDLAHQNVRVQEEG
                     QRIAESRFRNGAISELDVVQATNLLESTRATIPVLEIGQRQAQNALSTLLGRPTGYVQ
                     SLLGKPRGIPAPPARVAVSVPAEMLRRRPDIRGAELRAAAQCERIGIAKAQLYPSFVL
                     FGSIATQTTSGLGTSPITPTSSSFTNLFGPGSLAYNAGGSLFWPILSYRQLLNGVRVE
                     DARFQESLVAYVNTVLAAAQEVEDGMIGFLRQQDAAAFEQNAVTAAETSVRLALVQYR
                     EGAVDYQRVLDSQRALLDSQNRLASTRSEAVTALIALYKALGGGWELRQGDPVVPGPI
                     QREMQDRTHWNGYFSNPPGADAHSTGDPGDPGDPVPNRK"
     gene            2725..4164
                     /locus_tag="VF516_45280"
     CDS             2725..4164
                     /locus_tag="VF516_45280"
                     /inference="COORDINATES: protein motif:HMM:NF020921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115048.1"
                     /translation="MTTEPSTEARTRESPRSGAGSRAGEQGKESGRLDDELAKIDALW
                     RAANYLSVGQIYLLDNPLLRKPLARGHIKPRLLGHWGTTPGLNFLYVHLNRVIRKYDL
                     DMMYVIGPGHGGPALVANAYLEGTYSEVYPNISQDEDGMRKLFKQFSFPGGIPSHVAP
                     ETPGSIHEGGELGYALSHAYGAAFDNRDLFVIANPSYLARIPREELVKLLEGYGYRPY
                     FVEGHEPEAMHRQMAETLDLVVSEIHRIWKDARERGREGGRQSGRIDRPIWPTIVLPP
                     GSRVHRPRGQQEGRGDPGLPVPTLETLAAVSMVRRHLPQLRVRVINIVDLMRLQSADE
                     HPHGLADREFDALFTRDKPIIFAFHGYPSLIHQLTYRRSNHDNFHVHGFREEGTTTTP
                     FDMVVLNGLDRFHLALDVIDRLPQLGPRAAYFRQAIRDRLIDHKHYIEEHGEDLPEIT
                     GWRWERAAMPENPRGVSRGAATSTEGDNV"
     gene            4214..5413
                     /locus_tag="VF516_45285"
     CDS             4214..5413
                     /locus_tag="VF516_45285"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HEX8115049.1"
                     /translation="MNAGSSSLKCSVMESTDRTVLAQVKADWAGPVTRYERCGPGTAR
                     IREQVGFRDHGEAVRHVLEDLRTALGSAPAGERPAAVGHRIVHGGEFSAPVRITPAVR
                     SRISALAELAPLHNLPGLEALAEAETLLPDIPHVAVFDTAFHATLPPAARIYPLPHAW
                     TESWGIRRYGFHGLNHAYCTTRAAEMLGRPASELRLVICHLGHGCSATAVLHGHSIDT
                     TMGFTPLDGLMMATRSGSVDPGILTHVQRRHGLGAAEIDDALNQASGLFGVSGMSGDM
                     REVLAAGRAGHERARLAIAIYVHRVRQAIGALAVTLGGIDALVFTAGVGENAAEVRMA
                     SCQGLECLGVELDVDANARCHPDADVASRASRARVLVIAAREDLAMLSDVIRVVSAGV
                     SAGKECI"
     gene            5410..6297
                     /locus_tag="VF516_45290"
     CDS             5410..6297
                     /locus_tag="VF516_45290"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEX8115050.1"
                     /translation="MMDGPISVVLADVDGTLVTQDKVLTERARRAVQQLRERGIAFTI
                     TSGRPPFGMRMLVEPLGLTLPMAAFNGGALVLPDLTVLDERRLPASLLPGLIDLICAH
                     GLDVFLFRSHDWFVRSLDAPHVQRETATLQQPPAVVASFAGVLAGAVKVVGVSDDRPR
                     VAICEAAVQQAYGAQVSAARSQPYYLDVTHPTANKGVVIERLSRHLQVPLDRIAAIGD
                     QPNDVLMFERAGLSIAMGNASDEVKRRATFVTTSYADEGFAHAIEHIILPRAEPAGTR
                     SDTENAAPPAPNGSAPPRA"
     gene            6365..7159
                     /locus_tag="VF516_45295"
     CDS             6365..7159
                     /locus_tag="VF516_45295"
                     /inference="COORDINATES: protein motif:HMM:NF021391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HEX8115051.1"
                     /translation="MSSRHAWFIAGVALAASASPHPHVAQAQPAAQCAVQPKADALLR
                     KMSTDLADMQAFRFIADSVMEVVTRQGERIQSVAESTVWVQRPNKLRVDRMGPLGGAT
                     VYYDGSTLAVYGKRDNLYATAQAPGNLDDTIEFARDQLSIDAPGADLLSSNPYAVLTE
                     DVVSGRYLGIEPVGNRMCHHLAFRGHETDWQLWVEDGPRALPCRFVIVSKRETGAPQY
                     SVTTSDWNPAPGFAPETFAFAPPPDAGKIDFVALKDIARGAQQSRR"
     gene            7171..>7371
                     /locus_tag="VF516_45300"
     CDS             7171..>7371
                     /locus_tag="VF516_45300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115052.1"
                     /translation="MSTRHKRMLGGLAVIAALTVAGELTGTSPLLLGGGDAHAVIGRP
                     LTPMSYAGVARRTTRRAAYAGAV"
BASE COUNT         1111 a         2550 c         2567 g         1143 t
ORIGIN      
        1 cggcacctgg atcttcacgc ggaacatcag cttctctcgc tcgctccggg tctcgacctc
       61 cttgggcgtg aactgcgcct ccggcgacac gaagctcacg tatccggggg cgacccggtt
      121 gggctcgtag tcgacggtga accgggcatc ggcgccgatc ttcagggcag cggcctgctc
      181 ggacggcagg aagatctcca tgtagacgtc tcccagatcg accaccgtca gcgccttgcc
      241 gccggcggcc aggacctccc cgggctcggc caggcgatac agcactcgtc cgagcaccgg
      301 cgacgtgagc gtcgcatcgg tgatccggct ctggatcgtc gccacgttgg cctggccgac
      361 cttgacctgc tgctgggcga cctgcagctg ggcctgcgcc tcggcgcgcc cggcctggct
      421 ggtcttcagg gtcatcgtgc gcacgtcgta gtcgcgctgc gatccggcgt gctccgccag
      481 cagcttgcgc gcgcgctcct tctcgatccg ggcgagctcg atctcgctct gccgccggac
      541 gatgttggcc tgtgcgactg ccatctgctc gcgcgcggcc gccaccgctt gcctggcctc
      601 ggcgagctgc gagtccagcg tgacggtgtc catctggacc agcacctgcc ccggcttgac
      661 catgtcaccc tcggcgacga acacctcctt gaccttcagc ggcagcttgg acgcgacgtc
      721 gacctgcttg ctctcgatcc ggccgttgcc cgccgcgatg ccgctgggca gcgcggtctg
      781 cttcgccttc caatagcgaa ccccgaagaa cgcggcgatc gcggcggcga ccagcgcgag
      841 gatgatccat cttcgagatt tcttcgacat cgtgatctac tttcgattcg gcactggatc
      901 gccgggatca ccgggatcac cggtcgagtg ggcgtctgca cccggcgggt tcgagaagta
      961 gccgttccag tgggtccggt cctgcatctc gcgctggatg ggtcccggga cgacgggatc
     1021 gccctgacgc agctcccagc cgccgcccag cgccttgtag agcgcgatca gcgcggtcac
     1081 ggcctccgac cgcgtggagg cgagccggtt ctgcgagtcc agcagcgcgc gttgcgagtc
     1141 gaggacgcgc tggtagtcca ccgcgccctc gcgatactgc acgagcgcca gtcgcaccga
     1201 ggtctcggcc gcagtgaccg cgttctgctc gaacgccgcg gcgtcctgct ggcggagaaa
     1261 gccgatcatc ccatcctcga cctcctgggc tgccgcgagc acggtgttga cgtacgcgac
     1321 gagcgattcc tggaaccggg cgtcttccac ccggacgccg ttcaggagct gccggtagct
     1381 caggatcggc cagaacaggc tcccacccgc gttgtaggcc aggctgccgg ggccgaacag
     1441 gttcgtgaac gacgacgagg tgggggtgat gggcgatgtt ccgaggccgc tggtggtctg
     1501 ggtcgcgatc gaaccgaaca gcacgaagct gggatagagc tgcgccttgg cgatgccgat
     1561 gcgctcgcac tgcgcggcgg cccggagctc ggcgccccgg atatccggac gccgccgcag
     1621 catctcggcc ggcacgctga ccgcgacccg cgcgggcggc gccgggatgc cccgcggttt
     1681 gcccagcagc gactggacgt agccggtcgg gcgccccagc agcgtgctga gggcattctg
     1741 ggcctggcgc tggccgatct ccagcacggg gatggtcgcg cgcgtgctct cgagcaggtt
     1801 ggtcgcctgg acgacgtcga gctccgagat ggcgccattg cggaaccgcg attcggcgat
     1861 ccgctggccc tcctcctgca cccggacgtt ctgatgggcg agatcgatca gcacctcgaa
     1921 ggtgcggatg acgacgtagg tccgcgcgac ctctgcgctg agcgagacca gcgcgtcgtc
     1981 gtagtcggcc accgtcgcga gataggcggc gttcgccgct ctcaccccgc ggcgatactt
     2041 gccccagaag tcgagctccc agatcgcgtc gaacccgatc tggtattccc cgtaatggtg
     2101 attgatgaag ccaccgaagg agtgctcgct cggcttcacg acgccggcat gggcgatcgg
     2161 ccctggattg gtcggatact gctgtccgac tgcgatgccg accagcgctc gggcctcgag
     2221 gatccgcagc ccggcgacct gcagcgagag gttctggtga taggcgagct cgatcagctc
     2281 gtcgagcgtc ggatcatcga gcgtcttcca ccacgcgagc tcggtggcgg cgttgaccgc
     2341 gagccgcggg tcgtgactct cgctccagct cgtattcagg gcgaccttgg gtcgtacgaa
     2401 gtcggggccg accaggcatg cgggcgccgg cagcaccagg ccgatcacca gcagcgtgct
     2461 gacgaggtgg tcccgtacgt cggtctcctg atcgctcctc gaaacacgag atctcttcat
     2521 cggcgcgtgc gggtctgggg ctgcaaccga tgtgcctgac gcaccgtcgt ggtgtcccgt
     2581 gatcgttgct cgttcgccgg gcttggcggc acgacgtgtc gccaagcccg agcggccatg
     2641 ctcgcgcatg gatcaccgct cgtcggtgtc ccgaagaaca tgattcgtcg tcgcggctcg
     2701 cttcttgcga ccgaatcgat cgggatgacc accgaaccga gcaccgaagc aagaaccaga
     2761 gaatcacccc gctccggcgc aggctcgcgc gcgggagagc agggcaagga gtcaggcagg
     2821 ctcgatgacg agctcgcgaa gatcgatgcg ctctggcgcg cggcgaacta tctctccgtc
     2881 ggtcagattt acctgctcga caacccgctg ctgcgcaagc cgctcgcccg cgggcacatc
     2941 aagccgcgct tgctcgggca ctgggggacc acgccggggc tgaacttcct ctacgtgcac
     3001 ctcaaccgcg tgatccggaa gtacgacctc gacatgatgt acgtcatcgg gccggggcac
     3061 ggcggcccgg cgctggtcgc gaatgcgtac ctcgagggga cctacagcga ggtctatccg
     3121 aacatcagcc aggacgagga cgggatgcgg aagctgttca agcagttcag ctttccgggc
     3181 ggaatcccca gtcacgtcgc cccggagact cccgggtcga tccacgaggg cggcgagctc
     3241 gggtacgcgc tgtcgcacgc ctacggcgcg gcgttcgaca accgcgacct gttcgtcatc
     3301 gccaacccga gctacctggc gcggattccc agggaggagc tcgtcaagct gctcgagggc
     3361 tacggctacc ggccgtactt cgtcgagggc cacgagcccg aggcgatgca ccgccagatg
     3421 gccgagacgc tcgacctcgt ggtctcggag atccaccgga tctggaagga tgcgcgggag
     3481 cgcggccggg agggcggccg gcagagcggc cggatcgacc ggccgatctg gccgacgatc
     3541 gtcctgccac caggatcccg ggttcatcga ccacgtggtc aacaagaagg ccgaggtgat
     3601 ccgggtctac ctgtcccgac cctggagacc ctggcggcgg tgtcgatggt tcgccgccac
     3661 ctgccgcagc tccgggtccg cgtgatcaac atcgtcgatc tgatgcggct gcagtccgcc
     3721 gacgagcatc cgcacgggct cgccgatcgc gagttcgacg cgctgttcac ccgggacaag
     3781 ccgatcatct tcgcgttcca cggctacccg tcgctgatcc accagctcac ctaccggcgc
     3841 tccaatcacg acaacttcca cgtccatggc ttccgggagg aggggacgac cacgacgccg
     3901 ttcgacatgg tggtgctcaa cggcctcgac cgcttccacc tcgcgctcga cgtgatcgac
     3961 cggctgcccc agctcggacc gcgcgcggcg tacttccgcc aggcgatccg cgatcggctg
     4021 atcgatcaca agcactacat cgaggagcac ggcgaggacc tgcccgagat caccgggtgg
     4081 cgctgggagc gcgccgccat gccggagaat ccgcgcgggg tgagccgcgg cgccgcgacg
     4141 tccaccgagg gagacaacgt atgaccgcgg ctgcgcgagg cggcgatcgg agccgtgcgt
     4201 gagggtcgtg ctggtgaacg ctggctcgag cagcctgaag tgctcggtga tggagtcgac
     4261 ggaccgcacg gtgctcgcgc aggtcaaggc cgactgggcg gggccggtca cgcgctacga
     4321 gcggtgcgga ccggggacgg cgcggatccg cgagcaggtc gggttccgcg accacggcga
     4381 ggcggtgcgg catgtgctcg aggatctgcg tacggcgctg gggtcggcgc cggcgggcga
     4441 gcgaccggcg gcggtcgggc atcgcatcgt gcacggcggc gagttcagcg cgccggtccg
     4501 gatcacgccc gcggtccgct cgcggatctc cgcgctcgcc gagctcgcgc cgctgcacaa
     4561 cctgccgggg ctggaggcgc tcgccgaggc cgagacgctg ctcccggaca tcccgcacgt
     4621 cgccgtgttc gacaccgcgt ttcacgcgac cctgccgccc gcggcgcgga tctacccgct
     4681 gccccatgcc tggaccgaga gctggggcat ccggcgctac gggtttcacg gcctcaacca
     4741 cgcctactgc acgacccgcg cggccgagat gctcgggcgc ccggcgtccg agctgcgcct
     4801 ggtgatctgc cacctcggcc acggctgctc ggcgacggcg gtgctgcacg gccactcgat
     4861 cgacaccacg atgggcttca cgccactcga cgggctgatg atggcgaccc gcagcggctc
     4921 ggtggacccc gggatcctga cccacgtgca gcgccgccac ggcctcgggg ccgcggagat
     4981 cgacgacgcg ctgaaccagg cgtccggcct gttcggcgtg tccgggatgt cgggcgacat
     5041 gcgagaggtg ctggccgccg gccgggccgg ccacgagcgc gcccggctgg cgatcgcgat
     5101 ctacgtccac cgcgtgcggc aggcgatcgg tgcgctggcg gtgacgctcg gtggcatcga
     5161 tgcgctggtg ttcaccgccg gcgtcggaga gaacgcggcg gaggtccgca tggcgagctg
     5221 ccagggcctc gagtgcctgg gggtcgagct cgacgtcgat gccaacgccc gctgtcatcc
     5281 cgacgccgat gtggcgagtc gcgcatcgcg cgcgcgggtg ctggtgatcg ccgctcgcga
     5341 agatctcgcg atgctgtcgg acgtcatccg ggtggtctcg gcgggggtct cggccggcaa
     5401 ggagtgcata tgatggacgg accgatctcg gtcgtgctcg ccgacgtcga cggcacgctc
     5461 gtcacccagg acaaggtgct gaccgagcgg gcgcggcgcg ccgtgcagca gctgcgcgag
     5521 cgcgggatcg cgttcacgat cacgagcggc cgcccgccgt tcggcatgcg gatgctggtc
     5581 gagccgctcg ggctgaccct gccgatggcc gcgttcaacg gcggcgcgct cgtgcttccc
     5641 gatctgacgg tgctcgacga gcggcggctg ccggcgtcgc tgctgcccgg cctgatcgac
     5701 ctgatctgtg cccacggcct cgacgtgttc ctgttccgtt cgcacgactg gttcgtgcgc
     5761 tcgctcgacg cgccgcacgt gcagcgcgag accgcgaccc tccagcagcc gccggccgtc
     5821 gtggcgagct tcgcaggcgt gctggcgggc gccgtgaagg tcgtcggggt cagcgacgat
     5881 cgtccgcgcg tcgcgatctg cgaggccgcg gtccagcagg cctacggcgc gcaggtctcg
     5941 gccgcgcgct cgcagccgta ctatctggac gtgacccacc cgaccgcgaa caagggcgtg
     6001 gtcatcgagc gcctgtcgcg tcacctccag gtcccgctcg atcggatcgc cgcgatcggc
     6061 gatcagccga acgacgtgct gatgttcgag cgcgccgggc tcagcatcgc gatgggcaac
     6121 gcgagcgacg aggtcaagcg ccgggccacg ttcgtgacga cctcgtatgc agacgagggg
     6181 ttcgcacacg cgatcgagca catcatcctg ccgcgggccg aacccgcggg gactcggagt
     6241 gacaccgaga acgccgcgcc gccggccccg aacggctccg ctccgccgcg ggcgtgaggt
     6301 ccggcacccg atctgcaccg atcggcgtgc aggctctcgc tgtcgacgga gccaggagac
     6361 gatcatgagc tcaagacacg catggttcat cgcaggagtc gcgctcgccg cgagcgcatc
     6421 tccgcatccg cacgtggcac aggcgcaacc cgcggcgcaa tgcgcggtcc agccgaaggc
     6481 cgacgcgttg cttcgcaaga tgtccaccga cctcgccgac atgcaggcat ttcgattcat
     6541 cgcggacagc gtgatggagg tcgtcaccag gcagggcgag aggatccaga gtgtcgccga
     6601 gtccaccgtg tgggtgcagc ggccgaacaa gctgcgggtc gaccggatgg gaccgctcgg
     6661 tggtgcgacc gtctactacg acggcagcac gctcgcggtc tacggcaagc gagacaacct
     6721 gtacgcgacc gcgcaggcgc ccggcaacct cgacgacacc atcgagttcg cgcgcgatca
     6781 gctctcgatc gatgcgccgg gcgcggatct gctgtccagc aatccctacg cggtgctgac
     6841 cgaggatgtg gtgtccggca ggtacctggg catcgagccg gtgggcaacc ggatgtgtca
     6901 tcacctcgcg tttcgcggcc acgagaccga ctggcagctc tgggtcgagg acggcccgcg
     6961 cgcgctgccg tgccggttcg tgatcgtcag caagcgtgag accggcgcgc cccagtactc
     7021 ggtcacgacc tccgactgga acccggcgcc cgggtttgcc ccggagacgt tcgcgttcgc
     7081 accgccgccg gacgcgggca agatcgattt cgtcgcactg aaggacatcg cgcgcggcgc
     7141 gcaacaatcc aggagatgac catgacggca atgtccactc gtcacaagcg catgctgggg
     7201 ggcctcgcgg tgatcgcggc cctgaccgtc gcgggagagc tgaccggcac gagcccgctg
     7261 ctgctcggcg gcggcgacgc ccacgccgtg atcggcaggc cgctgacgcc gatgagctac
     7321 gccggggtgg cccggcgcac cacgcggcgg gcggcctacg ctggcgcggt c
//
LOCUS       DASVOR010002468         2250 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_465409,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002468 DASVOR010000000
VERSION     DASVOR010002468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465409"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1089
                     /locus_tag="VF516_45305"
     CDS             <1..1089
                     /locus_tag="VF516_45305"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEX8115053.1"
                     /translation="RTRMLRCGGALVGELRVGHDAAEVAAAIARFAAGGLDPILVLGA
                     SAIMDRRDVIPAALERAGGEVIRLGMPVDPGNLLMLGRLGAASVIGVPGCARSLKPSG
                     FDWVLQRVCAGVPIAAGDVAAMGVGGLLDEITIRPWPRSETPAGADGWPDDHAAGKPG
                     IAAVVLAAGRASRMGSNKLIAELDGEPIVRRTVRAVLGSRARPVIVVTGHEAEAVRAA
                     LAGLDVQFAHNPDFALGMSTSLRVGVAAAGPVAGALICLGDMPKLEARHLDTVIDACR
                     SGDPDEIIVPTCDRKRGNPVLWPQAYFAEIAELTGDVGARALIDRHAEQVRLLAIDDP
                     AILVDVDTPAALAELRGHAAGTGDPGRG"
     gene            1200..2210
                     /locus_tag="VF516_45310"
     CDS             1200..2210
                     /locus_tag="VF516_45310"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEX8115054.1"
                     /translation="MNKQLLERAAELARRGESFVFAVVVRREPYSSSQQGDSAIITAD
                     GSYHGWLGGNCTRPQVLREAAQALVDGKPRLIALSPEPDLQVRAGVTPLPMTCHSGGT
                     VDIFLEPVLAAPRLIVFGVSPVARAGAQLGTTMGYLVDVVDPDAHAAELPTTPFPADR
                     VFIDPAAAELRGGAPIASAVVASMGEHDEDAVAAALAMRPAYLGVVASRKRFAVLRET
                     LIERGISAHALDAIRSPAGLDLGGRQPEEVALSILAEIVQLKHAARPAAHAPAPVAQV
                     APAPTTAIDPVCHMTVTIATARHVGTWDGRTWYFCNPRCKDKFLADPKRYLEPSSSGA
                     AR"
BASE COUNT          281 a          820 c          852 g          297 t
ORIGIN      
        1 cgcacccgga tgctgcgctg cggcggagcg ctggtcggcg agctgcgggt cgggcacgac
       61 gccgccgagg tcgccgcggc gatcgcgcgg ttcgcggccg gcgggctgga tccgatcctc
      121 gtgctcggtg cgtcggcgat catggaccgg cgcgacgtca tcccggcggc gctggagcgc
      181 gccggcggcg aggtcatccg gctcggcatg ccggtggatc cgggcaacct gctgatgctg
      241 ggccggctcg gcgccgcgtc ggtgatcggc gtgccgggct gcgcgcgctc gctcaagccg
      301 tcggggttcg actgggtgct gcagcgcgtg tgcgcggggg tcccgatcgc cgccggggac
      361 gtggcggcga tgggggtcgg cgggttgctc gacgagatca ccatccggcc gtggccacgc
      421 agcgagacgc cggccggggc cgacggctgg cccgacgacc acgccgcggg caagccgggg
      481 atcgccgcgg tggtgctcgc cgccgggcga gcgagccgga tgggcagcaa caagctgatc
      541 gccgagctcg acggcgagcc gatcgtccgc cgcaccgtcc gcgcggtgct cgggtcgcgg
      601 gcgcgcccgg tgatcgtcgt gaccggccac gaggccgagg cggtgcgcgc cgcgctcgcc
      661 gggctcgacg tccagttcgc ccacaatccg gacttcgccc tcggcatgtc gacgtcgctg
      721 cgggtcggcg tggccgccgc ggggccggtg gccggggcgc tgatctgcct cggcgacatg
      781 ccgaagctgg aggcccgcca cctcgacacg gtgatcgacg cgtgccgctc gggcgatccg
      841 gacgagatca tcgtgccgac ctgcgaccgc aagcgcggca acccggtgct gtggccgcaa
      901 gcttatttcg ccgaaatcgc tgagctcacc ggggatgtcg gggcgcgggc gctgatcgat
      961 cgccacgccg agcaggtccg gctgctggcg atcgacgacc cggcgatcct cgtcgacgtc
     1021 gacacgccgg ccgcgctcgc cgagctgcgc ggccacgccg ccggcaccgg cgaccccggg
     1081 cgcggctgag ccggtcgcga cacactgttc acgtttcata aagatccgcg cggcgacgat
     1141 ccggtaaact cgcgggccgg atcgcgtgcg catggcactc gatctgctgc gaggagtcca
     1201 tgaacaagca gctgctagag cgtgcggcag agctggcgcg acgcggcgag tcgttcgtgt
     1261 tcgccgtggt ggtgcgccgc gagccgtaca gctcgtccca gcagggcgac tccgcgatca
     1321 tcaccgccga cggcagctac cacggctggc tcggcggcaa ctgcacgcgg ccccaggtcc
     1381 tgcgcgaggc ggcgcaggcg ctggtcgacg gcaagccgcg gctgatcgcg ctgtcgcccg
     1441 agcccgatct gcaggtccgc gccggggtga cgccgctgcc gatgacctgc cacagcggag
     1501 gaaccgtgga catcttcctg gagccggtgc tcgcagcgcc gcggctaatc gtgttcggcg
     1561 tctcgccggt cgcccgcgcg ggcgcccagc tcggcacgac gatgggctac ctggtcgacg
     1621 tggtcgatcc cgatgcccat gcggccgagc tgccgacgac cccatttccg gccgatcggg
     1681 tgttcatcga tccggcggcg gccgagctgc gcggcggcgc gccgatcgcc tcggcggtgg
     1741 tggcctcgat gggcgagcac gacgaggacg cggtggccgc ggcgctcgcg atgcgcccgg
     1801 cctacctggg cgtcgtcgcc agccgcaagc ggttcgcggt cctgcgcgag acgctgatcg
     1861 agcgcgggat ctcggcgcac gcgctcgacg cgatccggag cccggccggg ctcgacctcg
     1921 gcggccggca gcccgaggag gtcgcgctca gcatcctcgc cgagatcgtc cagctcaagc
     1981 acgcggcgcg ccccgcggcg cacgccccgg ccccggtcgc acaggtcgca cccgcgccga
     2041 ccacggcgat cgatccggtc tgccacatga cggtgacgat cgcgaccgcg cgccacgtcg
     2101 ggacctggga cggccgcacc tggtacttct gcaacccgcg ctgcaaggac aagttcctcg
     2161 ccgaccccaa gcgctacctg gagccgtcgt cgtcgggagc cgcgcggtga tccagccgcc
     2221 gcccggccgc caggcgcttg ccgacgagct
//
LOCUS       DASVOR010002469         1948 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_116029,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002469 DASVOR010000000
VERSION     DASVOR010002469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116029"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VF516_45315"
     CDS             complement(<1..952)
                     /locus_tag="VF516_45315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115055.1"
                     /translation="MLICAVASLVLILAGALFMDWFRAATEGFELNRLSIDLVAVRLC
                     GNAHVCARESVASVRGMYPSLAAVTLWSSLGFAALVGFQAGARLLAGGALDVFTKLGY
                     MLALMAMSLAVATAYLFGPEVPDVRMVATTWFDIALHRTWAPATLIAGLSIGFVAIYL
                     AVARESSDPAAAYKPVTLERSETGGGGPDGFAGGAGGRAPRGIDPSGTDRGRRVSIPF
                     PVPPGEQTDFMLAAPPREPTAVTRNEHPSQPGRHESTGSIRPHPPTQPLGSAPRTPNG
                     ALHTVEPGEHTGVLHAAHPTMRPPATAVRESTSTMRAARPA"
     gene            1161..1700
                     /locus_tag="VF516_45320"
     CDS             1161..1700
                     /locus_tag="VF516_45320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115056.1"
                     /translation="MPRDAPADPRGPARALVADTSVRAATTQARWLQPGELVRAQQPH
                     GSAAVLATGTTRIEAGARRERSHPALRPPGSHTPDPDDARRLTSLADLVDEVVACLDQ
                     PDGEPLRAAMARCNTELESYLEAQRAREPRFGGAQDELRGLARQLRSDRPDATLVVRQ
                     IRRVLGRVDAHRRGSSDAA"
BASE COUNT          294 a          715 c          712 g          227 t
ORIGIN      
        1 ccgcggggcg cgcggcgcgc atcgtgctgg tcgactcgcg gaccgcggtg gcgggcggcc
       61 gcatcgtcgg atgagccgcg tgcagcaccc cggtgtgctc gcccggctcg acggtgtgca
      121 gggcgccgtt cggcgtacgc ggcgcggatc cgagcggctg ggtcggcgga tgcgggcgga
      181 tcgagccggt cgactcgtgc cgcccgggct ggctcgggtg ctcgttgcgc gtcaccgcgg
      241 tcggctcgcg cggcggggcg gcgagcatga agtcggtctg ctccccgggg ggcacgggga
      301 acgggatcga cacccgccgg ccgcggtcgg tcccggacgg atcgatccca cggggggctc
      361 tgccccccgc cccgccggca aagccgtcgg ggcccccacc ccccgtctcg ctgcgctcga
      421 gcgtgaccgg cttgtaggcc gcggcggggt cgctcgactc gcgcgcgacg gccaggtaga
      481 tcgcgacgaa gccgatcgac agcccggcga tcagcgtggc gggcgcccag gtccgatgca
      541 gcgcgatgtc gaaccacgtg gtcgccacca tccgcacgtc gggcacctcg ggtccgaaca
      601 ggtaggcggt cgcgaccgcg agcgacatcg ccatcagcgc cagcatgtag ccgagcttgg
      661 tgaacacatc gagcgcaccg ccggcgagca gccgcgcacc ggcctggaac ccgaccagcg
      721 cagcgaagcc gaggctcgac cacagcgtca ccgcggcgag cgacgggtac atcccgcgca
      781 ccgaagctac cgactccctc gcgcatacat gcgcgttacc gcacaggcgc acggcgacga
      841 gatcgatcga gagccggttc agctcgaacc cctcggtcgc ggcccggaac cagtccatga
      901 acagcgcgcc ggcgaggatc aggacgagcg atgcgaccgc gcaaatcagc atacgcgtct
      961 gatcgcgtcc catcaccccg caacataaca cggctccggg ctgtaccaac gaacaggtgt
     1021 ggtgacaggt tggtctcgcc caccgagccg gcgcgcccgg ggcctcggca accggcggca
     1081 aggcccggac atgtgcaatg cacatgtccg gcggtctcgt cggggaccct cgacgggaga
     1141 tctacgataa cggaacagcg atgccgcgcg atgcgccggc agatcctcgg ggtcccgcgc
     1201 gggcgctcgt cgccgacacg tcggtccgcg cggcgacgac ccaggcacga tggctgcaac
     1261 ctggggagct cgtgagagca cagcaaccgc acggttcagc agcagtcctt gccacgggca
     1321 caacgcggat cgaggccggt gcgcgcaggg agcgtagtca tccggcgctg aggcctcccg
     1381 gcagccacac accggacccc gacgacgcac gacggctcac cagcctcgcg gacctcgtcg
     1441 acgaggtcgt ggcctgcctc gaccagccgg acggcgagcc gctgcgggcg gccatggccc
     1501 gctgcaacac ggagctggag tcgtatctgg aggcccagcg cgcgagagag ccgcgcttcg
     1561 gcggtgccca ggacgagctc cgcgggctcg cccgccagct gaggtcggat cggcccgacg
     1621 cgacgctcgt cgtgcggcag atccgccgcg tgctcggccg ggtcgatgcc caccgcaggg
     1681 ggtcgagcga cgcggcctga gcctggcgat cccggtgagc cggccgcgat ccccggcgtg
     1741 gctggcccgg gcaccatcgc atgagctaga accgccccat gggcgggcga agccccaccg
     1801 cgatgatcgc cgcccccggg cgcggcgcga tcgtgtgcgc ggcggtgatc gccgtcgcgg
     1861 cagccgcggt tccggcgcgc gcgcaccaga cctcggtcaa gtacgtcgac gtcaccgtcg
     1921 agggccggcg ggcgaccctc gcgctcac
//
LOCUS       DASVOR010002470         1851 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_231877,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002470 DASVOR010000000
VERSION     DASVOR010002470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231877"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..964
                     /locus_tag="VF516_45325"
     CDS             <1..964
                     /locus_tag="VF516_45325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase PknK"
                     /protein_id="HEX8115057.1"
                     /translation="EPLEARRDKIRAWAARHPGAGGMAFAETLGELIGTPFETVEREE
                     PGAVRDNPKLPSEQMRQSFLGFLREECATQPVVIVLEDLHWGDLSTIKLMYAALGALS
                     DQPLMVLALARNNVTELFPQLWADRCVQEIRLRRLSRRASAQLVRHALGDAVSEDAVK
                     ALVERSDRHASYLEALIRAAAAGDLDRTPAAVLAMVQSRLEKLDPAARRVLRAASIFG
                     TVFWRGGVEELIGAGDTAAWLAVLVDQGVIELKNETRFTDEVEYRFQHPLIREAAYET
                     LTNDDHIAGHWLAAQWLEKLGEDPRVVAAHFERCGRGGAPGTPP"
     gene            1029..>1851
                     /locus_tag="VF516_45330"
     CDS             1029..>1851
                     /locus_tag="VF516_45330"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8115058.1"
                     /translation="MERADLLEDRFEIEHQVRTGGMGAVFCARDRSSGERVAIKMLFD
                     GRDRRAARFARETQLLSELSHPGIVRYVAHGATPAGAPFLVMEWLDGEDLRRRLEREP
                     LTIGESVALTARVAEALAAAHARGIVHRDLKPSNLFLPDLRLDQVKVLDFGIAHKAGM
                     PQLTLTGTMVGTPGYMAPEQARSHEAVDARADVFALGCVLFHCLTGTAPFEGDSAIAV
                     LGKILFGEAPRVSALWPEVPEDLDALVARMLAKDPALRPSDGAALTAALAALGPRV"
BASE COUNT          243 a          654 c          702 g          252 t
ORIGIN      
        1 cgagccgctg gaggcgcggc gcgacaagat ccgcgcgtgg gccgcgcgcc accccggcgc
       61 cggcggcatg gcgttcgccg agacgctggg ggagctgatc ggcacgccgt tcgagaccgt
      121 ggagcgcgag gagcccggcg cggtgcgcga caaccccaag ctgccgagcg agcagatgcg
      181 gcagtcgttc ctcggcttcc tccgcgagga gtgcgcgacc cagccggtgg tgatcgtgct
      241 cgaggacctg cactggggcg atctgtcgac gatcaagctg atgtacgcgg cgctcggcgc
      301 gctctccgat cagccgctga tggtgctcgc gctcgcccgc aacaacgtga ccgagctgtt
      361 cccccagctg tgggccgacc gctgcgtgca ggagatccgg ctgcgccggc tgtcgcgccg
      421 ggcgagcgcg cagctggtgc gccatgccct cggcgacgcg gtgagcgagg acgcggtcaa
      481 ggccctggtc gagcgctcgg atcgccacgc gtcctatctc gaggcgctga tccgcgcggc
      541 cgccgcgggc gacctcgacc gcacgccggc ggcggtgctg gccatggtcc agtcccggct
      601 ggagaagctc gatccggcgg cgcggcgggt gctgcgcgcg gcgagcatct tcggcaccgt
      661 gttctggcgc ggcggcgtcg aggagctgat cggcgccggc gacacggccg cctggctggc
      721 ggtgctcgtc gatcagggcg tgatcgagct caagaacgag acgcggttca ccgacgaggt
      781 cgagtaccgc ttccagcacc cgctgatccg ggaagccgcc tacgagacgc tgaccaacga
      841 cgatcacatc gcgggccact ggctcgccgc gcagtggctg gaaaagctcg gcgaggaccc
      901 cagggtggtc gcggcgcact tcgagcgctg tgggcgaggc ggggcgcccg gcaccccgcc
      961 gtagggcaga ttacggaggg gccaggcctt gttgaccgcg ttcgcgcggg ggatggttcg
     1021 aaccggcgat ggagcgcgcg gatctgctgg aggatcggtt cgagatcgaa catcaggttc
     1081 gcactggtgg catgggggcg gtgttctgcg cccgcgaccg cagctcgggg gagcgggtcg
     1141 cgatcaagat gctgttcgac ggccgggacc gccgcgccgc gcggttcgcc cgggagactc
     1201 agctcttgtc cgagctgtcg caccccggca tcgtccgcta cgtcgcgcac ggcgccaccc
     1261 ccgcgggcgc gccgttcctg gtcatggagt ggctcgacgg cgaggacctc cggcgccggc
     1321 tggagcgcga gccgctcacg atcggcgagt cggtcgcgct caccgcccgg gtcgccgagg
     1381 cgctcgccgc cgcccacgcc cgcggcatcg tccaccgcga tctcaagccg agcaacctgt
     1441 tcctgcccga cctccggctc gaccaggtca aggtgctcga cttcggcatc gcccacaagg
     1501 cggggatgcc ccagctgacc ctgaccggga cgatggtcgg gacgccgggc tacatggcgc
     1561 ccgagcaggc gcgcagccac gaggcggtcg acgcgcgcgc cgacgtgttc gcgctcggct
     1621 gcgtgctgtt ccactgcctg accggcacgg cgccgttcga gggtgacagc gcgatcgcgg
     1681 tgctcggcaa gatcctgttc ggcgaggcgc cccgggtcag cgcgctgtgg cccgaggtgc
     1741 ccgaggacct cgacgcgctg gtcgcccgga tgctggccaa ggaccccgcg ctgcggccga
     1801 gcgacggcgc cgccctgacc gcggcgctgg cggcgctcgg cccgcgggtc c
//
LOCUS       DASVOR010002471         1789 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_289178,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002471 DASVOR010000000
VERSION     DASVOR010002471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289178"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..906
                     /locus_tag="VF516_45335"
     CDS             <1..906
                     /locus_tag="VF516_45335"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEX8115059.1"
                     /translation="DAAMFTRSTLKPLQAVPLVAAGGLERLGLDQRALALMCASHNGE
                     PEHVRVAAQILAAAGCRPEQLACGTHVPMFYLSNGNFPPPDEVPTPLQHNCSGKHAGM
                     LALCRLRGLGPDGYTDAAHPIQAVIREAVAAFAGIEPAAMASGPDGCSVPNYAFPLTA
                     LAGSYARLAAAGRDPVLGDIPGRLFAAMTEHPFLISGTGRQDAAISEAGRGDWVSKIG
                     GEGLQVIGIRSRGLGIAIKIADGDKGDYQPRATFPAAIEALRQLGVLSGELPGPLARF
                     DAAAQDHSEARFRVTLQPVFTLAAA"
     gene            complement(887..>1789)
                     /locus_tag="VF516_45340"
     CDS             complement(887..>1789)
                     /locus_tag="VF516_45340"
                     /inference="COORDINATES: protein motif:HMM:NF012503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8115060.1"
                     /translation="LGTGAVSWIAVDVRQRMDAAALREAMQRDARAGKRALLVIGTAG
                     TTGTGAIDPLPEIRDACREMGAWFHVDGSYGAMAAVLAEPPPELAALGDADSLAFDAH
                     KWMYLPCDVACALVRDPAVLAETFTYQPPYYSAKIRTIEGTRSYNALGPDNSRGFRAL
                     KVWLTLQQVGRAGYRALVGKDIALARLLHDQVRAHGELEPVSQSLGITTFRFVPRDLA
                     AGDHEDYLRRLNVAIVLRIQAEGRAFVSHAFVGERFVIRFCNVNFRTTAEDVAMLPAL
                     VVETGRQVDGELRHEPPPVTPRPA"
BASE COUNT          232 a          669 c          664 g          224 t
ORIGIN      
        1 gacgccgcga tgttcacccg gtcgacgctc aagccgctcc aggcggtgcc gctggtcgcc
       61 gccggcgggc tggagcggct cgggctcgac cagcgcgcgc tcgcgctgat gtgcgccagc
      121 cacaacggcg agccggagca cgtccgggtc gccgcgcaga tcctcgccgc ggcgggctgc
      181 cggcccgagc agctcgcgtg cggcacccac gtcccgatgt tctacctgtc gaacggcaac
      241 ttcccgccgc cggacgaggt gccgacgccg ctgcagcaca actgctcggg caagcacgcc
      301 ggcatgctcg cgctgtgccg gctgcgcggc ctgggtcccg acggctacac cgacgccgct
      361 cacccgatcc aggcggtgat ccgcgaggcg gtggcggcgt tcgccgggat cgagccggcg
      421 gcgatggcga gcgggccgga cggctgctcg gtgcccaact acgcgttccc gctgaccgcg
      481 ctggccggca gctacgcgcg gctcgccgcc gccggccgcg atccggtgct cggcgacatc
      541 ccgggccggc tgttcgcggc gatgaccgag catccgttcc tgatctcggg caccgggcgg
      601 caggacgcgg cgatcagcga ggccgggcgc ggcgactggg tcagcaagat cggcggcgag
      661 ggcctgcagg tgatcgggat ccgcagccgc ggcctcggca tcgcgatcaa gatcgccgac
      721 ggcgacaagg gcgactacca gccgcgcgcg acgttcccgg ccgcgatcga ggcgctgcgc
      781 cagctcggcg tgctgtccgg cgagctgccc gggccgctgg cgcggttcga cgccgccgcg
      841 caagaccaca gcgaggcgcg gttccgcgtg acgctccagc ccgtgttcac gctggccgcg
      901 gcgtgaccgg cggcggctcg tgccggagct cgccatcgac ctgccgcccg gtctcgacga
      961 cgagcgcggg cagcatcgcg acgtcctcgg cggtggtccg gaagttcacg ttgcagaacc
     1021 ggatcacgaa ccgctcgccg acgaacgcgt gcgacacgaa cgcgcggccc tcggcctgga
     1081 tccggagcac gatcgcgacg ttgagccggc gcaggtagtc ctcgtgatcg ccggccgcca
     1141 ggtcgcgcgg cacgaaccgg aacgtcgtga tccccagcga ctggctgacc ggctccagct
     1201 cgccgtgcgc gcggacctgg tcgtggagca gccgcgcgag cgcgatgtcc ttgccgacca
     1261 gcgcccggta gccggcgcgc ccgacctgct ggagcgtgag ccacaccttg agcgcgcgga
     1321 acccgcgcga gttgtcgggg ccgagcgcgt tgtagctccg ggtgccctcg atcgtccgga
     1381 tcttcgccga gtagtacggc ggctggtacg tgaaggtctc ggcgagcacg gccggatcgc
     1441 ggaccagcgc gcacgcgacg tcgcacggca ggtacatcca cttgtgggcg tcgaacgcga
     1501 gcgagtcggc gtcgcccagc gcggcgagct cgggcggcgg ctcggcgagc acggccgcca
     1561 tcgcgccgta cgagccgtcg acgtggaacc acgcgcccat ctcgcggcag gcgtcgcgga
     1621 tctcgggcag cgggtcgatc gcgccggtgc cggtggtccc ggccgtgccg atcaccagca
     1681 gcgcgcgctt gccggcccgg gcgtcgcgct gcatcgcctc gcgcagcgcc gcggcgtcca
     1741 tccgctgccg gacgtcgacc gcgatccacg acaccgcgcc ggtccccag
//
LOCUS       DASVOR010002472         3554 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_116057,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002472 DASVOR010000000
VERSION     DASVOR010002472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116057"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..952)
                     /locus_tag="VF516_45345"
     CDS             complement(<1..952)
                     /locus_tag="VF516_45345"
                     /inference="COORDINATES: protein motif:HMM:NF012796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl--tRNA ligase-related protein"
                     /protein_id="HEX8115061.1"
                     /translation="MPAYESIHSRHSQPAEPIAELSPDDHRALIARLDLCHFQDDAPA
                     MVFWHPRGFTLYRLLEDAARAHVVAAGYREVRTPQLLRRAVWERSGHWDAFHDAMFTV
                     GDLAGEAALKPVSCPGHIQLVRRLAPSWRDLPLRFAEMGLCHRDEASGSWHGLMRLRQ
                     FTQDDGHIFCTDDQVAGEVERFCRELPAFYRRFGFDDVSLALSLRPDNRLGDDAWWDR
                     AERELAEVVAGLGLPCEIQPGAGAIYGPKLEFILRDRTGRAWQCGTIQLDFVMPQRFD
                     VRYVDARGDRRHVVMLHRALFGSLERFLGILLEHHGAALPA"
     gene            complement(1219..3288)
                     /locus_tag="VF516_45350"
     CDS             complement(1219..3288)
                     /locus_tag="VF516_45350"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEX8115062.1"
                     /translation="MKLQLARILAPVLALVGPLAAGAPAAAKGLTIDDMLAMRRVGDP
                     AVSPDGKQVAFAVRDTDLEANRGRFDIWLAQTDGSGVKQLTTHPDNDQDPQWSADGSW
                     IYFLSSRSGSSQVWRIRPGGGEAEAVTKLPADVGGFRLFPDGKHLVVAVEVGPDARSI
                     GESVKRDEERARSKVKAKVYDQLLFRHWDHWEDGKFSHLFVWTSPEAGGKLDDARDLM
                     SGVAADAPTQPFGGMDEVSISPDGKSVAYVARVAGRENAWRTNTDVFVVPADGHGKPV
                     DVTAGNPAYDFGPAFSPDGKSLALRMMKRPGFEADRERIAVLDLASHKLRVVTEGWDR
                     SAGSIAWSPDGGTIYTDTDNVGNHSVFAIDVATGKARVVVDKGTNTAPRIAGDRLVFS
                     RDTLTSPAELYSVKPDGGELRQLTHLNDARVKAITWGDYQQFSFKGAKGDTVYGYVIK
                     PAGFSGSGGGKVPVAFLIHGGPQGSFGDHFHYRWNPEVFAGHGFGVVMVDFHGSTGYG
                     QAFTDAIANDWGGAPYDDLMMGLDAALDKNPWLDKSRVAALGASFGGYMINWINGKTD
                     RFKALVCHDGNLDERMAYFDTEELWFPEWEHGGLPWDKPEGYTKHNPIDLVKNWKTPT
                     LVIHGGQDFRVVDSQGMATFTALQRKGVASWFLYFPDENHWVVKPQNSKLWHEEVLAW
                     IDRWTKH"
     gene            complement(3285..>3554)
                     /locus_tag="VF516_45355"
     CDS             complement(3285..>3554)
                     /locus_tag="VF516_45355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115063.1"
                     /translation="PMVAVYLARRRQHRPLHADRFGKLATIAQFIALCVIALAPDHAL
                     PAAAVAGALGLVAAGHYIATAASVELHPERWTDRPAEAGRDTPRS"
BASE COUNT          505 a         1170 c         1354 g          525 t
ORIGIN      
        1 aggccggcag cgcggcgccg tgatgctcga gcaggatgcc gaggaagcgc tcgagcgagc
       61 cgaagagggc gcggtggagc atgacgacgt ggcggcggtc gccgcgcgcg tcgacgtagc
      121 ggacgtcgaa ccgctgcggc atcacgaagt cgagctggat ggtcccgcac tgccaggcgc
      181 ggccggtgcg gtcgcgcagg atgaactcga gcttggggcc gtagatcgca ccggcgccgg
      241 gctggatctc gcatggcagg cccaggcccg cgacgacctc ggcgagctcg cgctccgcgc
      301 ggtcccacca ggcgtcgtcg ccgagccggt tgtcggggcg cagcgacagg gcgagcgaga
      361 cgtcgtcgaa gccgaaccgg cggtagaacg ccggcagctc gcggcagaac cgctcgacct
      421 cgccggcgac ctgatcgtcg gtgcagaaga tgtggccgtc gtcctgggtg aactggcgca
      481 gccgcatcag gccgtgccag ctgccgctgg cctcgtcgcg gtggcacagg cccatctcgg
      541 cgaagcggag cgggaggtcg cgccaggacg gcgcgagccg gcggacgagc tggatgtggc
      601 ccggacagct gaccggcttg agcgcggcct cgccggcgag gtcgccgacg gtgaacatgg
      661 cgtcgtggaa ggcgtcccag tggccgctgc gctcccagac ggcgcgccgg agcagctgcg
      721 gtgtgcggac ctcgcggtag ccggcggcga cgacgtgggc gcgggcggcg tcctcgagga
      781 ggcggtacag ggtgaagccg cgcgggtgcc agaacaccat ggcgggggcg tcgtcctgga
      841 agtggcacag atcgagccgc gcgatcaggg cgcggtgatc gtcgggcgaa agctcggcga
      901 tcggctcggc tggctgggaa tggcgagaat ggatcgattc gtaggctggc atcgtcgtct
      961 ccgtgtcggt gggggaggcc acgggacggc cggtgcgcga cgacctcgac gactgcgccc
     1021 ggtgccttgt ggccgtccgg gcgtgggtcg tgtcgtgggg ttcagccagc gcgcacggcc
     1081 acctgcccga aacaggcggt ggtcgtggtc gtcgtgagac gcgcagagct gaacacggtc
     1141 acgagaacct aggaacggat cgccgagctg gcaaggggca atttcgcgcc ggccctgcga
     1201 caggtgccgc tgtcgcggtc agtgcttggt ccagcggtcg atccaggcga ggacttcctc
     1261 gtgccacagc ttggagttct gcggcttgac cacccagtgg ttctcgtcgg ggaagtacag
     1321 gaaccagctc gccacgccct tgcgctgcag cgcggtgaag gttgccatgc cctggctgtc
     1381 gacgacgcgg aagtcttgcc cgccgtggat caccagggtc ggggtcttcc agttcttcac
     1441 caggtcgatc gggttgtgct tggtgtagcc ctcgggcttg tcccacggca ggccgccgtg
     1501 ctcccactcg gggaaccaca gctcctcggt gtcgaagtac gccatgcgct cgtcgaggtt
     1561 gccgtcgtgg cagaccaggg ccttgaagcg gtcggtcttg ccgttgatcc agttgatcat
     1621 gtagccgccg aacgaggcgc cgagcgcggc gacgcggctc ttgtcgagcc acgggttctt
     1681 gtcgagcgcg gcgtcgaggc ccatcatcag atcgtcgtac ggcgcgccgc cccagtcgtt
     1741 ggcgatcgcg tcggtgaacg cctggccgta gccggtcgag ccgtggaagt cgaccatgac
     1801 gacaccgaag ccgtggccgg cgaacacctc ggggttccag cggtagtgga agtggtcgcc
     1861 gaagctgccc tgcgggccgc cgtggatcag gaacgcgacc gggaccttgc cgccgccgga
     1921 acccgaaaac ccggcgggct tgatgacgta gccgtacacg gtgtcgccct tggcgccctt
     1981 gaacgagaac tgctggtagt cgccccaggt gatcgccttg acccgcgcgt cgttgaggtg
     2041 ggtgagctgg cggagctcgc cgccgtcggg cttgacgctg tacagctcgg ccggcgaggt
     2101 cagcgtgtcg cgcgagaaca cgaggcgatc gccggcgatg cgcggcgcgg tgttggtgcc
     2161 cttgtcgacg acgacgcggg ccttgccggt cgcgacgtcg atcgcgaaca ccgagtggtt
     2221 gccgacgttg tcggtgtcgg tgtagatcgt gccgccgtcg gggctccacg cgatcgaccc
     2281 ggccgagcga tcccagccct cggtgacgac gcgcagcttg tgcgacgcga ggtcgagcac
     2341 ggcgatccgc tcgcggtcgg cctcgaagcc cgggcgcttc atcatccgca gcgcgagcga
     2401 cttgccgtcg ggcgagaacg ccgggccgaa gtcgtaggcc gggttgccgg cggtgacgtc
     2461 gaccggcttt ccgtggccgt cggccgggac gacgaacacg tcggtgttgg tgcgccacgc
     2521 gttctcgcgg ccggcgaccc gggcgacgta cgccaccgac ttgccgtcgg gcgagatcga
     2581 gacctcgtcc atcccgccga acggctgggt cggcgcgtcg gcggcgacgc cggacatgag
     2641 gtcgcgggcg tcgtcgagct tgccgccggc ctccggggag gtccacacga acaggtgcga
     2701 gaacttgccg tcctcccagt ggtcccagtg gcggaacagc agctggtcgt agaccttggc
     2761 cttgaccttg gaccgcgcgc gctcctcgtc gcgcttgacc gactcgccga tcgacctggc
     2821 gtcgggcccg acctcgaccg cgacgaccag gtgcttgccg tcggggaaca gccggaagcc
     2881 gccgacgtcg gccggcagct tggtcacggc ttccgcctcg ccgccacccg gccggatccg
     2941 ccacacctgc gacgagcccg agcgcgacga caggaagtag atccagctgc cgtcggccga
     3001 ccactgcggg tcctggtcgt tgtcggggtg ggtggtgagc tgcttgaccc cggagccgtc
     3061 ggtctgggcc agccagatgt cgaaccggcc gcggttggcc tcgaggtcgg tgtcgcgcac
     3121 ggcgaacgcg acctgcttgc cgtcgggcga caccgccggg tcgccgaccc gccgcatcgc
     3181 gagcatgtca tcgatggtga ggcccttcgc ggcggcgggc gcgccggccg ccaggggtcc
     3241 aacgagcgcg agaaccggcg cgagaatgcg cgcgagctgt agcttcatga ccgtggagta
     3301 tcacggccgg cctcggccgg gcggtccgtc cagcgctcgg gatgcagctc gacgctcgcc
     3361 gcggtcgcga tgtagtggcc ggccgcgacc aggccgagcg cgccggcgac ggccgccgcc
     3421 gggagcgcgt ggtccggggc cagcgcgatg acgcacagcg cgatgaactg cgcgatggtc
     3481 gcgagcttgc cgaaccggtc ggcatgcagc gggcggtgct gccgccggcg cgccaggtac
     3541 accgcgacca tcgg
//
LOCUS       DASVOR010002473         1569 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_378031,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002473 DASVOR010000000
VERSION     DASVOR010002473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378031"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..>1569
                     /locus_tag="VF516_45360"
     CDS             125..>1569
                     /locus_tag="VF516_45360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase"
                     /protein_id="HEX8115064.1"
                     /translation="MAIRLAAAGFRTTVFEARDQPGGRAYVYRDGGFVFDGGPTVITA
                     PPCLEELFAAAGRRLADCVELIPVAPFYRLVWPDGDRLDYSGDADHMIAQIRRRNPAD
                     AEGYRRFVEYSRAVFDAGYTGLASTPFLRFRDMVRVAPELIRLRADRSVYGAVARFVR
                     DEHVRQALSFHSLLVGGNPFDTSAVYTLIHHLERKWGVFFPRGGTGALVAALVRLLEE
                     LGGELRLGTPVERVAVTGTRRGVRHLVTADRIAEAFDLVVSNADLHHTYAALYRGVAG
                     ADRMRRRIERLDWSMSLFVLYFGTDRPYTGEVVHHTVLFGPRYRGLLDDIFHGRALPR
                     DFSLYLHAPTVTDPSLAPPGCGAFYVLAPVPHLGNAPLDWAALATGYADRILAALEGV
                     LPDLRRHVVVRRTLTPQGFATELRAHLGSAFSCAPRLGQSAWFRPHNRDPRIPRLYLV
                     GAGTHPGAGVPGVINSAAATARIIGRDFGVEA"
BASE COUNT          168 a          598 c          560 g          243 t
ORIGIN      
        1 cccgggcacg ccgcgctcgc gcgggtcgat gccgagctca tccagctgga gcgcgatgcg
       61 ggctagcccc agcgccggcg ggccgcgcgc cgccatcatc gggtccggct tcggcggcct
      121 ggcggtggcg atccggctcg ccgccgccgg gttccggacc acggtgttcg aggcgcgcga
      181 tcagccgggc gggcgcgcct atgtctaccg cgacggcggc ttcgtgttcg acggcgggcc
      241 cacggtgatc accgcgccgc cgtgcctcga ggagctgttc gcggcggcgg gccgccggct
      301 ggccgactgc gtcgagctca tcccggtcgc gccgttctac cgcctggtgt ggcccgacgg
      361 ggatcgcctc gactacagcg gcgacgccga tcacatgatc gcgcagatcc ggcggcgcaa
      421 cccggccgac gccgagggtt accggcgctt cgtggaatac agccgcgcgg tgttcgacgc
      481 cgggtacacc gggctcgcgt cgaccccgtt cctgcggttc cgcgacatgg tgcgggtcgc
      541 ccccgagctg atccggctgc gtgccgatcg ctcggtgtac ggcgcggtcg cgcggttcgt
      601 ccgcgacgag cacgtccggc aggcgctgag ctttcactcg ctgctggtcg gcggcaaccc
      661 gttcgacacc agcgcggtct acaccttgat ccaccatctc gagcgcaagt ggggcgtgtt
      721 cttcccgcgc ggcgggaccg gcgcgctggt cgccgcgctg gtccggctgc tcgaggagct
      781 cggcggcgag ctgcggctgg gcacgccggt cgagcgcgtc gccgtgaccg gcacgcggcg
      841 gggagtccgc cacctcgtca ccgccgaccg gatcgccgag gcgttcgatc tcgtcgtatc
      901 caacgccgat ctccaccaca cctacgcggc gctgtaccgc ggcgtcgccg gcgccgatcg
      961 catgcggcgc cggatcgagc ggctcgactg gtcgatgagc ctgttcgtcc tctacttcgg
     1021 cacggatcgg ccctacaccg gtgaggtcgt tcaccacacc gtgctgttcg ggccgcgcta
     1081 ccggggcttg ctcgacgaca tcttccacgg ccgcgcgctg ccgcgcgact tcagcctgta
     1141 tctccacgcg ccgaccgtca ccgatccttc gctggcgccg ccgggctgcg gtgcgttcta
     1201 tgtcctcgcg ccggtgcccc acctcggcaa cgccccgctc gactgggccg cgctcgccac
     1261 cggctacgcc gatcggatcc tcgcggcgct cgagggcgtg ctgcccgatc tgcgccgcca
     1321 cgtggtggtc cggcgcacgc tgacgccgca gggcttcgcg accgagctgc gcgctcacct
     1381 cggctcggcg ttctcgtgcg cgccgcggct cggccagagc gcgtggttcc ggccgcacaa
     1441 tcgcgatccg cggatcccgc ggctctacct cgtgggcgcc gggacgcacc cgggcgcggg
     1501 ggtgcccggc gtcatcaact ccgcggcggc gaccgcgcgg atcatcggcc gcgacttcgg
     1561 cgtggaggc
//
LOCUS       DASVOR010002474         2489 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_554080,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002474 DASVOR010000000
VERSION     DASVOR010002474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554080"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(271..771)
                     /locus_tag="VF516_45365"
     CDS             complement(271..771)
                     /locus_tag="VF516_45365"
                     /inference="COORDINATES: protein motif:HMM:NF012305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEX8115065.1"
                     /translation="MGQIEQAMNTRLYVGNLSFNTNAEGVRAAFQEFGTVSDVHLVSD
                     RETGRSRGFAFVTMGSPEEAAKAIEGMDGRTLDGRPLRVNEAEQRQARGGGGGGGGFR
                     SGGGGYGGGGGGYGGGGRGGDRGGYGGGGGGDYGGGGGGGGDYGDGGGRRGGRGGRGG
                     RGGDRW"
     gene            1032..1601
                     /locus_tag="VF516_45370"
     CDS             1032..1601
                     /locus_tag="VF516_45370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8115066.1"
                     /translation="MSEPERTLLRRLRDRDERAFRELVETHRDRVFNITYRMLGDRAE
                     AEDVAQEVFISVFKTIDTFREEARFSTWLYRVTVNHCKNRIKYLARRHDRDRDELDET
                     SHETNGAAGVPRHAQPDRALEGAQLEVLLQEAIGHLDDDQRVVVVLRDVEDLSIEEIC
                     EITGLPDGTVKSRLHRARLVLRKRLQRHV"
     gene            1633..2091
                     /locus_tag="VF516_45375"
     CDS             1633..2091
                     /locus_tag="VF516_45375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115067.1"
                     /translation="MIDDALHDGEAGPVGEEIDDAILARVSDYLDGALTGPERTEVAG
                     KIASDATWKRAHDELTETRNYLSGLRKAHAPPSFAEHVTETIHQRSAGRLFARKTLGD
                     RVPFGVLLVIALAGLVVIAYVLWASATGSLKVDRPRDRPPTAGSSAVAPP"
     gene            2248..>2489
                     /locus_tag="VF516_45380"
     CDS             2248..>2489
                     /locus_tag="VF516_45380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115068.1"
                     /translation="MKQCTTCGRRWDIVGLNCPDDGAPLVPAEAASAGRTASATWSAA
                     QPALAQLRPAASGELAPGDCVGEYVIERRIGAGGMG"
BASE COUNT          413 a          916 c          827 g          333 t
ORIGIN      
        1 cgtccagcga gtccgcgccg gcgacgcggc cgccggcgag cgcggcgagg cacgacacac
       61 cgagcacgag cgagcgcatg gcgaagtgtt ggcgtgatcc gcggccgggc gcaagaaacc
      121 cgcgcgcgtg ccgatctggc accgcggcgg ccggccggac cgccgccgga gaccccgccg
      181 gagacccgaa aacgacagcg cggccgctac cgggccgcgc tcctgacctg cctcgtcctt
      241 cgatcccctg cctcgtcctt cgaacgcggg ttaccagcga tcgccgccgc gaccgccgcg
      301 accgccgcga ccaccgcgcc ggccaccgcc gtcgccgtag tcaccaccac caccgcctcc
      361 gccgccgtag tcccctccac cgccaccccc gtagccaccg cggtccccgc cgcggccgcc
      421 gccgccatag ccaccaccac cacccccgta gccgccgccg ccgctacgga agccgccgcc
      481 accaccgcca ccgccgcgcg cttgacgctg ctccgcctcg ttgacgcgaa gcggacgacc
      541 gtccagcgtg cggccgtcca tcccctcgat cgccttggcg gcttcctcgg ggctgcccat
      601 ggtcacgaag gcaaagccgc gggagcggcc ggtctcacgg tccgagacca agtggacatc
      661 gctgaccgtt ccgaactcct ggaacgcggc acgtacccct tctgcgttgg tattgaacga
      721 taggtttccg acatacagac gagtgttcat cgcttgctcg atctggccca agtgacaacg
      781 ttcccgccca gagggggccg tgggtgctcc aaagcggggg tttccaagac aaaaaggcgc
      841 tgcttcaaac tcccactgag caccgggaca aaaaccgacg aaccgtggca agccgaagct
      901 actcctcgcg gtgggtgatt gccaccagga acgtcgactg cagctcaacc acccggaacg
      961 acacgggata aatgtaagaa actcacgcag accggcggtg tcggaggatc ggacgagtat
     1021 gctcgacgga catgtctgag ccggagcgaa cgctgctgcg gcgcctgcgg gaccgcgatg
     1081 agcgagcgtt ccgtgagctg gtcgagacgc accgcgatcg cgtcttcaac atcacctacc
     1141 gcatgctcgg cgaccgcgcc gaggccgagg acgtcgcgca ggaggtgttc atctccgtgt
     1201 tcaagacgat cgacaccttc cgcgaggaag ccaggttctc gacctggctc taccgggtca
     1261 ccgtcaacca ctgcaagaat cgcatcaagt acctcgcgcg ccgccacgat cgcgatcgcg
     1321 acgagctgga cgagacctcg cacgagacca acggcgccgc gggggtgccg cgccacgcgc
     1381 aacccgatcg cgcgctcgag ggcgcacagc tcgaggtcct gctccaggaa gcgatcggac
     1441 acctcgacga cgatcagcgc gtggtcgtgg tgctgcgcga cgtcgaggac ctgtcgatcg
     1501 aggagatctg cgagatcacc ggcctgcccg acggcacggt gaagtcgcgg ctgcaccgcg
     1561 cgcgcctggt gctgcgcaag cggctgcagc gccatgtctg accgatgcgc taccctggag
     1621 gtacccgtgc ccgtgatcga tgatgctctg cacgacggcg aagccggccc ggtgggcgag
     1681 gagatcgacg acgcgatcct cgcccgggtg tccgactacc tcgacggcgc gctgaccggg
     1741 cccgagcgca ccgaggtcgc gggcaagatc gccagcgatg cgacctggaa gcgcgcgcac
     1801 gacgagctga ccgagacccg caactacctg tccgggctgc gcaaggccca tgcgccgccg
     1861 agcttcgccg agcacgtcac cgagaccatc caccagcgca gcgcgggccg gctgttcgcg
     1921 cgcaagacgc tcggtgatcg cgtgccgttc ggcgtgctgc tcgtgatcgc gctcgccggg
     1981 ctggtggtga tcgcgtacgt gctgtgggcg tcggcgaccg gctcgctcaa ggtcgaccgc
     2041 ccgcgcgacc gcccccccac cgccggatcg tccgcggtgg cgccgccgtg atcacgcgtt
     2101 gatcacgctc ccgtccggct ccgtgatctc cgacgcgcgg tgaggatcgg tgtggcacca
     2161 gggcacaggg ggtgggtcgg cgcggcgacg ccgatcacgc cggtgatctc gggactaccg
     2221 atcgcatgat aatttgattc gcaggcgatg aagcagtgca cgacctgcgg acgccgatgg
     2281 gacatcgtcg gcctgaactg tcccgacgac ggcgcaccgc tcgtgcctgc cgaggccgcg
     2341 agcgcgggcc gaacggcctc ggcgacatgg tccgccgcgc agccggcgct cgcccagctc
     2401 cggcccgcgg cgtcgggcga gctcgctccc ggcgactgcg tcggcgagta cgtgatcgag
     2461 cggcggatcg gcgccggcgg catgggcat
//
LOCUS       DASVOR010002475         1827 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_435825,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002475 DASVOR010000000
VERSION     DASVOR010002475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1827
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435825"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /gene="gatC"
                     /locus_tag="VF516_45385"
     CDS             complement(<1..287)
                     /gene="gatC"
                     /locus_tag="VF516_45385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEX8115069.1"
                     /translation="MPALTRKEVEDIALLARLHLESDELERMEHELAAILDHFQTIAA
                     VDTTDVPAMTHAVPMDLRLRADAPEPSLPAEAALAAAPRRSGDLIVVPSSIA"
     gene            complement(418..1161)
                     /locus_tag="VF516_45390"
     CDS             complement(418..1161)
                     /locus_tag="VF516_45390"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HEX8115070.1"
                     /translation="MLKPVEKQRVAEEIAEQLRSLILNGQYPPGSKLPPERELSKRLR
                     VNRASLREALKKLEHLGLVRIRQGDGTRVQNFMETGGIELVQHLLPLAGGKPELIRDL
                     LEFRRIFGRELARLAAARATKDKVGLGKLRSLADRADQVASALELFELDFDFYVAIAQ
                     LSGNQVMLLLINTVRDGVRGFMPLLANLAAPGDAVRKHHRDLIAALEKGDVASAGRVA
                     DDYLRLGAELAGRIGGKPELQPQPLPEPR"
     gene            1264..1548
                     /locus_tag="VF516_45395"
     CDS             1264..1548
                     /locus_tag="VF516_45395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115071.1"
                     /translation="MSGRPAPSSEASPPRVLVVDGGGDHEVFEVVAAVGDVIRARSAF
                     LFEIGEVLSVRIVHDGRVSDATARVRAHTGPDEARITELEISERSAPRAG"
     gene            1551..>1827
                     /locus_tag="VF516_45400"
     CDS             1551..>1827
                     /locus_tag="VF516_45400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115072.1"
                     /translation="MVVAELDEVTYDLEVDGEEVRRTLHRRIWQRGGWATVAIAYQER
                     AAGGDWKPAKLALIRFQRVRDVWQRHAAITLRGGDAVALGETLAAWRD"
BASE COUNT          248 a          627 c          688 g          264 t
ORIGIN      
        1 gcgatgctcg acggcacgac gatcaggtcg ccggaccggc ggggcgcagc ggcgagcgcg
       61 gcctcggccg gcagcgacgg ctcgggggcg tcggcgcgca gccgcaggtc catcggcacc
      121 gcgtgggtca tcgccggcac gtcggtcgtg tcgaccgcgg cgatggtctg gaagtggtcg
      181 aggatcgccg cgagctcgtg ctccatgcgc tcgagctcgt cggactccag gtggagccgc
      241 gcgagcaacg cgatgtcctc gacttccttc cgggtcagcg ccggcatcgc ctcatctcta
      301 ctctctgcac cggtctactc tctgcatctg gctacgcctg gatctaccgc cggggtctac
      361 ccctgagaac cccggtcggc atcctatcat cacgcgcggg cgtggacgca cggcgcgcta
      421 ccgcggctcc gggagcggct ggggctggag ctccggcttg ccgccgatcc ggccggccag
      481 ctcggcgccg agccgcaggt agtcgtcggc gacccgaccg gcgctggcga catcgccctt
      541 ctcgagcgcg gcgatcaggt cgcggtggtg cttgcgcacg gcgtcgccgg gcgccgcgag
      601 gttggcgagc agcggcatga acccgcgcac gccgtcgcgc acggtgttga tcaggagcag
      661 catgacctgg ttgcccgaca gctgcgcgat cgcgacgtag aagtcgaagt cgagctcgaa
      721 cagctcgagc gcgctggcca cctggtcggc gcggtcggcg agcgagcgca gcttgccgag
      781 cccgaccttg tccttggtcg cgcgcgccgc ggcgagccgg gcgagctcgc ggccgaagat
      841 ccggcggaac tccagcagat cgcggatcag ctcgggcttg ccgccggcca gcggcaacag
      901 gtgctggacc agctcgatcc cgccggtctc catgaagttc tggacccggg tgccgtcgcc
      961 ctggcggatc cggaccaggc cgaggtgctc gagcttcttc agggcctcgc gcagcgacgc
     1021 ccggttgacc cgcagccgct tggacagctc gcgctcgggc ggcagcttcg agcccggcgg
     1081 atactgacca ttgaggatca gcgagcgcaa ctgttcggcg atctcctcgg ccacacgttg
     1141 cttctcgacc ggtttgagca tccgctgtga gagaggttaa cccagcgcgg tcagtgatag
     1201 aaggccccgg gagcgcggga tggatgcttg aggcggcggc ggggagcatg gtaccgacac
     1261 ggggtgtccg gccggcccgc gccgagcagc gaagcgtcgc cgcctcgcgt cctggtggtc
     1321 gacggcggcg gcgatcacga ggtgttcgag gtcgtggcgg ccgtcggcga cgtgatccgg
     1381 gcgcgctcgg cgttcctgtt cgagatcggc gaggtgctca gcgtacggat cgtgcacgat
     1441 gggcgagtct cggacgccac cgcgcgggtc cgcgcccaca ccggccccga cgaggcgcgg
     1501 atcaccgagc tcgagatctc cgagcgctcg gcgccgcgcg cagggtagcc atggtggtcg
     1561 ccgagctcga cgaagtcacc tacgacctcg aggtcgacgg cgaggaggtc cgccgcacgc
     1621 tccaccgccg gatctggcag cgcggcgggt gggcgactgt cgcgatcgcc taccaggagc
     1681 gcgcggccgg cggggactgg aagccggcga agctggcgct gatccggttc cagcgcgtgc
     1741 gcgacgtctg gcagcgccat gcggcgatca cgctgcgcgg cggcgacgcg gtcgcgctcg
     1801 gcgagaccct ggcggcgtgg cgcgacg
//
LOCUS       DASVOR010002476         1878 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_465516,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002476 DASVOR010000000
VERSION     DASVOR010002476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1878
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465516"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VF516_45405"
     CDS             complement(<1..180)
                     /locus_tag="VF516_45405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115073.1"
                     /translation="MVLRYVVLDFDGTCTQIELAHAGFLADYLAVLEDANGRARGALQ
                     PAWDAAVVRVRAASPD"
     gene            complement(236..622)
                     /locus_tag="VF516_45410"
     CDS             complement(236..622)
                     /locus_tag="VF516_45410"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8115074.1"
                     /translation="MLIVDDYPDAAEATCMLFTRLGHLCRCAISGEHALDEAKAFLPD
                     IVILDIGLPDLSGYEVARTLRQRHGTAVYLAALTGWGQPEDRRRAIDAGFDQHIVKPA
                     TAQVLCELIAAAERRRDQLGLPPPSA"
     gene            complement(691..>1878)
                     /locus_tag="VF516_45415"
     CDS             complement(691..>1878)
                     /locus_tag="VF516_45415"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEX8115075.1"
                     /translation="RSETGGGDPDGFAGGAGGRAPRGIERSETGGGDPDGFAGGAGGR
                     APRGIERSEMGGGIECGERHGAELGPPGVTWGFLMGCELDARERAELPMLAPGHGDRL
                     RAGVRQVITERAGQRAELDLEIDGTSRTFDFRIEPTAFGATAVGFDITPSKVAEAALR
                     DADRRKDEFLATLSHELRNPLAPLQVALEAARLLDDDPRQRAHSLSIMERQIAMLTTL
                     VNELLDLSRVTQGKIVLERAIVSVAHALESAVVATRPMFEAARHELRVNVPDRPVEVD
                     GDFRRLVQVFTNLLTNAAKYTPPSGHIDVAIVPGPASVAVRVQDDGLGITSDMLPRIF
                     DIFVQSSETHGRSQGGLGIGLSLVRRLVELHGGTVTAVSAGAGHGSAFTVELPLHAGG
                     SPA"
BASE COUNT          258 a          707 c          647 g          266 t
ORIGIN      
        1 gtcgggcgac gcggcgcgga cccggaccac cgcggcgtcc catgccggct gcagcgcacc
       61 gcgcgcacgg ccgttggcgt cctccaggac cgcgaggtag tccgcgagga agccggcgtg
      121 cgccagctcg atctgcgtgc aggtgccgtc gaagtccagg accacgtagc ggaggaccat
      181 ccgcgccagc atacgcggaa accgcggtcc gctgagctcc cggatcggcg gacgatcacg
      241 ccgagggcgg aggcagcccg agctggtcgc gccggcgctc ggccgcggcg atcagctcgc
      301 acagcacctg tgcggtggcg ggcttgacga tgtgctgatc gaacccggcg tcgatcgcac
      361 gccggcgatc ctcgggctgc ccccagccgg tcagcgcggc gaggtacacc gccgtgccgt
      421 gccgctggcg cagcgtgcgc gcgacctcgt agccgctgag atcggggagg ccgatgtcga
      481 ggatgacgat gtcgggcagg aacgccttgg cctcgtcgag cgcgtgctct ccgctgatcg
      541 cgcaccggca gaggtgcccg agccgggtga acagcatgca ggtcgcctcg gccgcgtccg
      601 gataatcatc gacgatcaac acgcggtgcc ggtacttcgc aggctccagt gccggcgcat
      661 gcgacgaggt gctgcgaggt ccgctcgcca tcatgcagga cttcctccgg cgtggagtgg
      721 aagctcgacg gtgaacgcgc tgccgtgccc ggcgcccgcg ctcaccgccg tgaccgttcc
      781 gccgtgcagc tcgaccagcc ggcgcaccag gctgaggccg atcccgagcc cgccctgcga
      841 gcgcccgtgg gtttcgctcg actgcacgaa gatatcgaag atgcgcggca gcatgtcgga
      901 tgtgatcccc agtccatcat cttgcacgcg cacggccacg ctcgcgggcc ccgggacgat
      961 cgcgacatcg atgtggccgc tcggtggcgt gtacttcgca gcattggtca gcaggttggt
     1021 gaacacctgt accagcctgc ggaagtcgcc gtcgacctcg accggccggt ccggaacgtt
     1081 gacccggagc tcgtgccggg cggcttcgaa catcggcctg gtcgcgacca ccgcgctctc
     1141 cagcgcgtgc gccacgctga cgatcgcgcg ctccagcacg atcttgccct gcgtgacgcg
     1201 cgacaggtcg agcagctcgt tgaccagcgt ggtcagcatc gcgatctggc gctccatgat
     1261 cgacaggctg tgcgcgcgct gccgcgggtc gtcgtcgagc agacgcgcgg cctcgagcgc
     1321 gacctggagc ggcgccaggg ggtttcgcag ctcgtgcgac aaggtcgcga ggaactcgtc
     1381 cttgcggcga tcggcgtcgc gcagcgcggc ctcggcgacc ttgctcggcg tgatgtcgaa
     1441 gcccaccgcg gtcgcaccga acgcggtcgg ctcgatccgg aagtcgaacg tgcgcgacgt
     1501 cccgtcgatc tcgaggtcga gctccgcgcg ctggccggcg cgctcggtga tcacctgacg
     1561 gacgccggcg cgcagccggt ccccgtgccc gggcgccagc atcggcagct cggcgcgctc
     1621 gcgggcatcg agctcgcacc ccatcaggaa tccccaggtc acgcccggcg gaccgagctc
     1681 cgccccatgg cgctcgccac actcgatccc accccccatc tcgctccgct cgatcccccg
     1741 gggggctctg ccccccgccc cgccggcaaa gccgtcgggg tccccccccc ccgtctcgct
     1801 ccgctcgatc ccacgggggg ctctgccccc cgccccgccg gcaaagccgt cggggtcccc
     1861 accccccgtc tcgcttcg
//
LOCUS       DASVOR010002477         8525 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_289247,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002477 DASVOR010000000
VERSION     DASVOR010002477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 8525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8525
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289247"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2109
                     /locus_tag="VF516_45420"
     CDS             <1..2109
                     /locus_tag="VF516_45420"
                     /inference="COORDINATES: protein motif:HMM:NF016736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEX8115076.1"
                     /translation="APLADLGAPAVRVMLRPIQLAAGAPYTTRYTELSALRLVGVRVR
                     VDASGLCPQRLQSDERMLDPAKPFMPFGAAPALGSWLRIGHSELSGKPLDQLQLDLEW
                     MGLPGDLATHYGAYLRKDKSRLVDVDKIAVDLSLIDRGQPTKLRLGAALFGATSSISV
                     PAISPPAGNLDPDALSAAALPDELASWRRTLQCVLVGSDFGHAVYPALAASKAVDLAT
                     DVANQKPDLDADSYKVNPPYTPKLKSLSLTYSASLAIRLDAGPPDAGVARILYVHPFG
                     QCDVHRELGATPPAAGVPFLPRYDNDGELYLGLTDVAPPQSVALLFQLAEGSADPDLP
                     AQPVVWSYLDGDCWRPFAHRVLRDTTGGLTSAGIVELALDPVAPSARFGDGLYWIRAA
                     VEQNTASVCDTIDIHPQAVSAVFVDHDNAPDHFRTPLSPGSIKKLSSRIAGIAGVRQP
                     YTSRGGRAAEDPSRFATRVSERLRHKQRALSMWDYERLILENFPQVYKARCLPAPATA
                     PGKVEIIVIPDIHDLLPSDPFEPKAPSSLLGEIANYLRGRMPAFADVVVRNPRYVSVR
                     VRLSVRFRNAGNDAFYIRTLNDDLNRFLSPWAFDDGSDIVLGGRIYANSIVDFVDRRD
                     YVDYVAGVQLFWRTADGDYQVIPPDQGDEGYFVTAASPDAVLVAARTHEITVLTEAAF
                     DETSLFGINFMKVGLDFFVG"
     gene            2134..4260
                     /locus_tag="VF516_45425"
     CDS             2134..4260
                     /locus_tag="VF516_45425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115077.1"
                     /translation="MDINKKNREYLRGRFKKNAIPTQDDFADFIEAAINLKDDGLVRP
                     PGDALSVEASAAGAHPVLKLYDAQAAAAAWVVNLLTGDPAAGKKGLVISDNAGNSRLS
                     IDQSTGELTTAALTAKGAVTAGGGLTIPATQTASVGAVTASGAITANGGLTIPATQTL
                     TAQGAASIAGPLRMNKTVLGIGTPNGSLAAANEVVGAIGFYGYGRSNGQLSYRAGAGF
                     ELVDRSSDAPSLDYVVDGSRPYADLKVRTLTASDGVRVDGNRSTHLDVDGALYRTGSQ
                     VYLTVDDSLFVRRSAAAAVFQLDTTTGRLGVGKTSPACAVDVQGDIHATGALEVQGDI
                     RTTGQLVAPGGISLGNDKFSLTMRPHATYVDPKTGQKVMYPVLHLKGVVLTSDVKARN
                     AIACTRLDVNAVSGDASRAGAITCATLTASGDINAHGFNTGGTTTTGWLTATLGVNAP
                     SVHADSVVTSGPVTAHGAMIATGTLDVVAAPADPTRPGAITCATLTASGDINAKGGIT
                     AGSVIKAPGVNATSVQAGSLQTDGQILASSRIVAMGGMIAIGTLDVNPVSGDPNRPGR
                     ITCGELATSGHKNFEIAHPLDPAHKTLSHASLEGPEAGVYYRGEAQLRDGRAVVTLPP
                     YFEALTRKDGRTVMITPRCDGDDPISPLAATGITDGCFLVRAIDRQNLSQRFYWEVKA
                     VRGDVAALEVERRKPMTEDPSNPVIP"
     gene            4436..6433
                     /locus_tag="VF516_45430"
     CDS             4436..6433
                     /locus_tag="VF516_45430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115078.1"
                     /translation="MASQLLPNAMDYAALREEGIRQLERLSGPWTDFNTHDPGITLLE
                     QLCYALTDLGYRAAHELPDLLAEDGADPYRSLHLPEQVLTCSPVTPADLRRVVLDVDG
                     VQNAWVEPLAGDTQIVYYHLGPKTLSLSPTPLPSEPVPLQGLYRVLVDPKLGASSDLR
                     ANVALRLHRSRGLCEDFAEITVLEPQKITVDAVVEIDAVDDPGELGRRLVQQIADVIS
                     PPVPFCSLDEALRMGLSLDAVLDGPRLAHGFVSDDALKRATRRTAVNTSDLIHAVRDL
                     PEVRAVSRLRIFTSEQTSSWSLAIPPGKVAKLDRDLSSIVLRNAGKVVARVASAGTAS
                     AALDTPVPGAGLTRPAGRDRHVLRYTSVQRHLPAIYGVGDHGLVDSAAPERKSRANQL
                     RAYLMFFDQLMANHLAQLAHVKDLLSHDDVATTTYFAQPVEQPDRAADVLRDRSEQDL
                     LASSLAAGDPAGAEPLDLARRNRFLNHLLSRFAETVGEHGALAPEALLRAKQAFLRGY
                     PVLGGARGAGFSYLEPAGPDNGIALEQRIALKLGLTTHERLRIVEHILLRPMEGDELQ
                     QVPLVTAPFAKDPYSLQLTFALRSGTSQDVGRFADDEFRTLVEQTIREETPAHLTAYV
                     RWIDAADWPAFETAHTAWLDRRREYWAAQLGLTLAPEVSPP"
     gene            6430..7986
                     /locus_tag="VF516_45435"
     CDS             6430..7986
                     /locus_tag="VF516_45435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115079.1"
                     /translation="MTTRHVHVRATRDRVIDLLGLGETYPLQDVAVEQDQLTVSFQQP
                     TSFGIEPIQRDVRYELYDDDHHPVDPPCWLDGSRDDTRDDTRLPGPIVTADRTFRIHA
                     VKLATGHDDFLAQTASVKVGLNTQLRAYLPDLPRLSDDAGDPMDSDPRIADHGASVRV
                     IVEGTQAEASYQLVYAGPDGTEIITAPVPSTGGALALVTRPVEEDTRIRIRATRMSGP
                     PDTALLAPVLPLAVRADPLRPVAIDGGAIVDPAAATLVIGASQASVLYTAHVRTLRDH
                     DFVDGAPPGESVFTVAVPAALDAPAHDVQVRMPALPVPWQVVQDDFSPRGATTPGSIS
                     GGEIRILLGALGEDSVLVIQAHKDHFAADAGPQPVRSGRSDVQLTTPLVALARPDAAP
                     ALTFTLQPSDEGAGSALLVSGGQRGVFYHLRATASGPELGLPAYVHRLDDDDPEQNRG
                     IGQLAIQYDLALARASASAVLPSPELVRPPDPLVDVAPVPGAAIDVHAVASRTGIPWP
                     ASRTVPVVRP"
     gene            7983..>8525
                     /locus_tag="VF516_45440"
     CDS             7983..>8525
                     /locus_tag="VF516_45440"
                     /inference="COORDINATES: protein motif:HMM:NF039901.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system tape measure
                     protein"
                     /protein_id="HEX8115080.1"
                     /translation="MIAQRHRIRRQVLEIQIAEAPAAWPLQSALSRLHGQELAAIVER
                     CCTEVGDPGRLHRIDALEVDLGRLDPDHLERDLIERLGPALRQALALRIQRDDADLAS
                     AGRDPELESQLELVAHFACQGYLPWWADRSQRRIVDEALDVLVRRAAPSLSRLVRALA
                     RACTPLQRLVAHTDDVRLSAV"
BASE COUNT         1296 a         3176 c         2848 g         1205 t
ORIGIN      
        1 gcgccgctcg cggacctcgg cgctcccgcc gtgcgcgtga tgctgcggcc gatccagctc
       61 gccgccgggg cgccgtacac cacccggtac accgagctct ctgcgctccg gctggtcggc
      121 gtgcgcgtgc gggtggacgc cagcgggctg tgtccccagc ggctccagag cgacgagcgg
      181 atgctggacc ctgcgaagcc gttcatgccg ttcggcgccg cgccggccct cggatcgtgg
      241 ctccgcatcg ggcacagcga gctgtcgggc aagccgctgg accagctcca gctcgacctc
      301 gagtggatgg gactccccgg ggacctggcc actcactacg gcgcctacct gcggaaggac
      361 aaatcgcgtc tggtcgacgt ggacaagatc gccgtcgacc tgtcgctgat cgatcgcgga
      421 cagccgacga agctgcgcct gggggctgcg ctgtttggcg ctacgtcctc gatctcggtg
      481 cctgcgatct ccccgcccgc cggcaacctc gatcccgacg cgctgtcggc cgccgcgctc
      541 cctgacgagc tcgcgagctg gcgccgcacc ctgcaatgcg tgctggtcgg ctccgatttc
      601 ggccatgcgg tgtaccccgc gctcgcggcg agcaaggcgg tcgacctcgc caccgacgtc
      661 gcgaaccaga agccggatct cgacgcggac agctacaagg tgaacccacc gtataccccc
      721 aagctcaaga gcctgtcgct cacctactcc gcctcgctgg cgatccggct ggacgccggc
      781 ccgcccgatg ccggcgtcgc acgcatcctg tacgtccatc cgttcggcca gtgcgatgtg
      841 caccgcgagc tcggcgccac cccgcccgcc gccggggtgc cgttcttgcc gcgctacgac
      901 aacgacggcg agctctacct cggcctgacc gacgtggccc cgccgcagag cgtcgcgctg
      961 ctgttccagc tcgccgaggg cagcgccgat cctgacctgc cggcccagcc cgtggtgtgg
     1021 agctacctcg acggcgactg ctggcggccg ttcgcgcacc gcgtcctgcg cgacaccacg
     1081 ggcgggctca cgagcgcggg gatcgtcgag ctcgcgctcg atccggtggc gccgagcgcg
     1141 cggttcggcg acggcctcta ctggatccgc gccgcggtgg agcagaacac ggccagcgtg
     1201 tgcgacacga tcgacattca cccgcaggcg gtatcggcgg tattcgtcga ccacgacaat
     1261 gcgcccgatc acttccgcac cccgctgagc ccgggctcga tcaagaagct gagctcccgg
     1321 atcgccggga tcgccggcgt gcgtcagccc tacacgtcgc gcggcggccg cgcggccgaa
     1381 gatccgagca ggtttgccac gcgggtcagc gagcggcttc gccacaagca gcgcgcgctg
     1441 tcgatgtggg actacgagcg gctcatcctc gagaactttc cgcaggtcta caaggcgcga
     1501 tgcctccccg cccccgccac ggcaccgggg aaggtcgaga tcatcgtcat cccggatatc
     1561 cacgatctcc tgccgtccga cccgttcgag cccaaggccc cgtcgagcct gctcggcgag
     1621 atcgcgaact acctccgagg caggatgccc gcgttcgccg acgtggtggt ccgcaatccg
     1681 cgatacgtgt cggttcgcgt gcggctatcc gttcgattcc gcaacgcagg caacgacgcg
     1741 ttctacatcc ggacgctcaa cgacgatctc aaccggttcc tgtcgccctg ggcgttcgac
     1801 gacggcagcg acatcgtcct cggcgggcgg atctacgcca acagcatcgt cgacttcgtc
     1861 gaccgccgcg actacgtcga ctacgtcgcc ggggtccagc tgttctggcg cacggcagat
     1921 ggcgactacc aggtcatccc tcccgatcag ggcgacgagg ggtacttcgt caccgcggcg
     1981 agccccgacg ccgtcctcgt cgccgccagg acgcacgaga tcacggtgct cacggaggcg
     2041 gcgttcgacg agacatcgct gttcgggatc aacttcatga aggtggggct cgacttcttt
     2101 gtcgggtgat gcgctccgcc acgcgaggca gccatggata tcaacaagaa gaatcgcgag
     2161 tatctcaggg gacgcttcaa gaagaacgcg atcccgacgc aggatgactt cgccgacttc
     2221 atcgaggcgg cgatcaacct gaaggatgac gggctcgtcc ggccgccggg ggacgcgctc
     2281 agcgtcgagg cctccgccgc gggtgcgcat cccgtgctca agctgtacga cgcgcaggct
     2341 gcggccgcgg catgggtggt caatctgctc accggcgacc cggccgccgg caagaagggc
     2401 ctggtcatca gcgacaacgc ggggaactcc aggctgtcga tcgatcagag caccggcgag
     2461 ctcacgacgg ccgcgctcac cgcgaagggc gcggtcaccg ccggtggcgg gctcacgatc
     2521 ccggcgaccc agacggcctc ggtcggcgcc gtgaccgcat ccggcgcgat caccgccaat
     2581 ggcgggctca cgatcccggc gacccagacg ctcacggccc agggcgcagc atcgatcgcc
     2641 ggcccgctgc ggatgaacaa gaccgtgctc ggcatcggca cgccgaatgg cagcctcgcc
     2701 gcggcgaacg aggtggtcgg cgcgatcggc ttctacggct acggccggtc gaacgggcag
     2761 ctatcgtacc gggccggcgc cggattcgag ctcgtcgatc gctcgagcga cgcaccgtcg
     2821 ctggactacg tcgtcgatgg atcgcggccc tacgccgatc tcaaggtgcg aacgctcacc
     2881 gcgagcgacg gcgttcgcgt cgacggcaac cgcagcacgc acctcgatgt cgatggcgcg
     2941 ctgtaccgca ccggcagcca ggtctacctc accgtcgatg acagcctgtt cgtccgccgc
     3001 agcgccgccg cggcggtgtt ccagctcgat accaccaccg ggcggctcgg cgtgggcaag
     3061 accagcccgg cgtgcgcggt cgatgtccag ggcgacatcc acgcgaccgg cgcgctcgag
     3121 gtccagggtg acatccggac cacgggacag ctggtcgccc ccggcgggat ctcgctcggc
     3181 aatgacaagt tctcgctcac gatgcgcccg cacgcgacgt acgtcgaccc gaagaccggc
     3241 cagaaggtca tgtaccccgt gctgcacctc aagggcgtcg tgctcaccag cgacgtcaag
     3301 gcgcgcaacg ccatcgcgtg cacccggctc gacgtcaacg ccgtgtccgg cgacgccagc
     3361 cgtgccggcg cgatcacgtg cgccaccttg accgcgagcg gcgacatcaa cgcgcacggc
     3421 ttcaacacgg gcggcacgac caccacgggc tggctcactg cgacgctcgg cgtcaacgcc
     3481 ccctcggtcc atgcggactc ggtcgtgacg agcggtccgg tgactgccca cggtgcgatg
     3541 atcgccaccg gaacgctcga cgtggtcgcg gcccccgccg accccacccg ccccggcgcg
     3601 atcacgtgcg ccacgctgac cgcgagcggc gacatcaacg cgaagggcgg catcaccgcc
     3661 ggcagcgtca tcaaggcccc cggagtcaac gcgacctcgg tccaggccgg atcgctccag
     3721 accgacggtc agatcctggc cagtagccgg atcgtggcca tgggcgggat gatcgccatc
     3781 ggaacgctcg acgtgaaccc ggtctccggc gaccccaatc gtccggggcg gatcacgtgc
     3841 ggcgagctcg cgacctcggg ccacaagaat ttcgagatcg cgcatccgct cgacccggcg
     3901 cacaagacgc tgtcgcacgc gtcgctggag gggcccgagg ccggagtcta ctaccgcggc
     3961 gaggcgcagc tccgcgacgg ccgcgcggtc gtcacgctgc cgccgtactt cgaggcgctc
     4021 acccgcaagg acggccgcac ggtcatgatc acgccgcgct gcgacggcga tgatccgatc
     4081 tcaccgctcg ccgccaccgg gatcaccgac ggctgcttcc tggtgcgcgc catcgatcgc
     4141 cagaacctgt cccagcggtt ctactgggag gtcaaggcag tgcgcggaga tgtcgcggcg
     4201 ctcgaggtgg aacgccgcaa accgatgacc gaggacccct cgaacccggt gatcccatga
     4261 ccacgcaggt cctgcgcgcc acagggtcca ccacagggtc cgaaacccgg tcccgaaaac
     4321 aagccacggc ggatgtgagt ccgccgtggt cgattcggaa gggcgattcc gtagggcgat
     4381 tcgcttggtc tcaagataag tccggccggg tgaccgcgca aggagaatga tcggtatggc
     4441 gagccagcta ctcccgaacg cgatggacta cgcggctctc cgcgaggagg gaatccgcca
     4501 gctcgagcgg ctatcgggac cgtggaccga cttcaacacc catgatccgg gaatcacgct
     4561 cctggaacag ctgtgttacg cgctcacgga cctcggctac cgggcggcgc acgagctccc
     4621 cgatctgctg gccgaagatg gcgcggaccc ctaccggagc ctgcatctcc ccgagcaggt
     4681 cctgacctgc agtccggtga caccggccga tctgcgccgc gtggtgctcg acgtcgatgg
     4741 tgtccagaat gcatgggtcg agccgctcgc aggtgacacg cagatcgtgt actaccacct
     4801 cgggccgaag acgctcagtc tgtcgccgac gccgctgccg tccgagccgg tgccgctcca
     4861 gggcctgtat cgcgttctcg tcgatccgaa gctcggcgcc agcagcgacc tccgcgcaaa
     4921 cgttgcgctc cgcctgcacc gcagccgcgg gttgtgtgag gacttcgccg agatcaccgt
     4981 gctcgagccg cagaagatca cggtcgatgc cgtggtcgag atcgacgcgg tggacgaccc
     5041 cggcgagctc gggcgccggc tggtccagca gatcgccgac gtgatctccc cgccggtccc
     5101 gttctgcagc ctggacgagg cgttgcgcat gggcctgtcg ctcgacgcgg tgctcgatgg
     5161 cccgcgcctc gcacacgggt tcgtatcgga cgacgcgctg aagcgcgcga cgcggcggac
     5221 cgcggtcaac acgtccgacc tcatccacgc cgtccgcgat ctcccggagg tccgggcggt
     5281 cagccggctg cggatcttta cctcggagca gaccagcagc tggtcgctcg cgatcccgcc
     5341 cggcaaggtg gccaagctcg atcgtgatct ctcgtccatc gtgctccgga acgccggcaa
     5401 ggtcgtggct cgcgtggcga gtgcagggac cgcgagcgct gcgctggaca ctccggtgcc
     5461 cggcgccggc ctcacccgtc cggccggccg cgatcgccat gtcctgcgct acacgtcggt
     5521 ccagcgccac ctcccggcga tctacggagt cggcgaccac ggccttgtgg actcggctgc
     5581 gccagagcgc aagtcgcgcg cgaaccagct ccgggcctac ctgatgttct tcgatcagct
     5641 catggccaac cacctggccc agctcgccca tgtcaaggat ctgctgtcgc acgacgacgt
     5701 ggcgaccacg acctactttg cgcagcccgt ggagcagccc gatcgggcgg ccgacgtgct
     5761 ccgcgaccgg agcgagcagg acctcctggc gagcagcctc gccgctggcg acccggcggg
     5821 cgccgagccg ctcgacctcg cgcgcaggaa ccggtttctc aaccacctgc tgtcccggtt
     5881 tgccgagacc gtcggcgagc acggtgcgct cgcgccggag gcgctgctgc gtgcgaagca
     5941 agcgttcctg cgcggctatc ccgtgctcgg cggcgcgcgc ggcgcggggt tcagctacct
     6001 cgagcccgcc ggcccggaca acggcatcgc gctcgagcag cggatcgcgc tcaagctcgg
     6061 gctcaccacc cacgagcgcc tgcggatcgt ggagcacatc ctgctccgtc cgatggaagg
     6121 cgacgagctg cagcaggtgc cgctggtcac cgcgccgttc gcgaaggacc cctactcgct
     6181 ccagctgacc ttcgccctgc ggtcgggcac atcacaggac gtcggccggt tcgccgacga
     6241 cgagttccgc accctcgtcg agcagaccat tcgcgaggag acgcccgccc acctgacggc
     6301 gtacgtgcgc tggatcgacg cggccgactg gccggcgttc gagaccgctc acacggcgtg
     6361 gctcgaccgc cggcgtgagt actgggccgc gcagctcggc ctgacgctgg ccccggaggt
     6421 ctcgccgcca tgaccacccg acacgtccac gtgcgcgcta cccgcgatcg cgtcatcgac
     6481 ctgctcgggc tcggcgagac ctacccgctg caggacgtcg cggtcgagca ggaccagctc
     6541 acggtcagct tccagcagcc cacctcgttc ggcatcgagc ccatccagcg cgacgtccgc
     6601 tacgagctgt acgacgacga ccaccacccc gtggatcccc cctgctggct cgacggcagc
     6661 cgggacgaca cccgggacga cacccgcctg cccggcccga tcgtcaccgc ggaccggacc
     6721 ttccgcatcc acgcggtcaa gctcgcgacg ggacacgacg acttcctggc ccagacggcg
     6781 agcgtcaagg tcgggctcaa cacgcagctc cgcgcctacc tgcccgatct cccgcggctg
     6841 tccgacgacg ccggcgatcc gatggacagc gatccccgga tcgccgatca cggcgcgagc
     6901 gtccgcgtga tcgtggaagg cacccaggcc gaggcgagct atcagctggt ctacgccggc
     6961 cccgacggga ccgagatcat caccgctccg gtgccgtcga ccggcggcgc gctggcgctg
     7021 gtcacgcggc cggtcgagga ggacacccgg atccggatcc gggctacccg gatgtcggga
     7081 ccaccggata cggcgctgct cgcccccgtg ctgccgctcg cggtgcgcgc cgaccccctg
     7141 cggccggtcg cgatcgacgg cggggcgatc gtcgatccgg cggcggccac gctcgtgatc
     7201 ggcgcgagcc aggcgagcgt cctgtacacc gcccacgtcc ggacgctccg cgatcacgac
     7261 ttcgtggacg gcgcgccgcc gggcgagtcc gtgttcaccg tggcggtccc cgccgcgctg
     7321 gacgcgcccg cccacgacgt ccaggtccgc atgccggcgc tgccggtccc ctggcaggtg
     7381 gtgcaggacg acttctcgcc tcgcggcgcg accacgccgg gcagcatcag cggcggcgag
     7441 atccggatcc tgctcggagc gctcggcgag gacagcgtcc tggtgatcca ggcgcacaag
     7501 gaccacttcg cggccgacgc cgggccacag ccggtgcgat ccggccggtc ggatgtccag
     7561 ctcacgacgc cgctcgtggc gctggcccgc cccgacgccg cgccggcact cacgttcacg
     7621 ctgcagccga gcgacgaagg cgcgggcagc gccctgctgg tctcgggcgg gcagcgcggc
     7681 gtgttctatc acctccgcgc gacggcgagc ggccccgagc tcgggttgcc ggcctacgtg
     7741 caccggctcg acgacgacga tcccgagcag aaccgcggga tcggccagct cgcgatccag
     7801 tacgacctcg cgctcgcccg cgcctccgcg tccgcggtgc tgccgagccc cgagctggtc
     7861 cggccccccg atccgctggt cgatgtcgcg ccggtaccgg gcgccgcgat cgacgtccac
     7921 gcagtcgcat cgcgcaccgg catcccctgg cccgcgagcc ggacagtccc ggtggtgagg
     7981 ccatgatcgc gcagcgccac cgcatccgcc gccaggtcct cgagatccag atcgcagagg
     8041 cgccggcggc atggccgctc cagagcgccc tgagccggct gcacggccag gagctcgcgg
     8101 cgatcgtcga gcgctgctgc accgaggtcg gcgatccggg acgccttcac cgcatcgacg
     8161 cgctggaggt cgatctcggc cggctcgacc cggaccatct ggagcgcgac ctgatcgagc
     8221 ggctcgggcc ggcgctgcga caggcgctcg cgctgcggat ccagcgcgac gatgctgacc
     8281 tcgcgagcgc ggggcgcgat ccagagctcg agtcccagct cgagctggtc gcacacttcg
     8341 cttgccaggg gtatctcccc tggtgggcgg atcgatcgca gcgccgcatc gtggacgaag
     8401 cgctcgacgt gctggtgcgc cgcgcggcgc cgtcgctctc gcggctggtg cgcgcgctcg
     8461 cgcgcgcgtg cacgccgctg cagcgcctgg tggcccacac cgacgacgtg cggctgtccg
     8521 ccgtg
//
LOCUS       DASVOR010002478         2365 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_406297,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002478 DASVOR010000000
VERSION     DASVOR010002478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406297"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2365
                     /locus_tag="VF516_45445"
     CDS             <1..>2365
                     /locus_tag="VF516_45445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115081.1"
                     /translation="VELANAPPRPGAPSRALVDALVEVTSTQLGTIDPRSLFEPGELR
                     RARRWAVVAVGVNLAFLVIEPRMVADGWRSLVLAHPAPFDGARLSAVPLVGDLEATLT
                     FPAYSRRPRLTLPSSSGDVRGLPGTTVALRARVLVPAASAELIIEPIDRGEPRTISAQ
                     LDGDQLTAALTIDRSARYRFAISSPSGERAIETAPRAIEAEPDQAPAVQLMAPADPLD
                     VTTLRRVELAYVIEDDFGITSAELVWESGRDKGKKPITLDSTRGGAAAAGAGAGAGLG
                     ASGSPVPRGRVQGKLMWDIAELQVPSGGEVRYWIEAKDNDTVGGPNIGRSRELHLKVV
                     SPRERHEETLGRQQALAEKILKNLGGRLTLGDEPAARDDLARQLREAVIELGTIGAAF
                     EKDPHASDALRKALSQMRERLDKLALAEQRLLPKGKPPARTAFAALDPRMVGELEDDT
                     IVLADWLDRERLESLLDVSDEIAAHQKRLADLLAQYQRSKDPRLLDEIEREMHALDRA
                     YAELDRHRRGMAEDVLDQYVHRSAVQAQAGTSCIDEVRKLVRAGEAARAQQKLEQCRQ
                     QQERAASSLEGSLAGMRGDKFSDEQKKLDEVMNELADVAKEQDDIASESNRIFESYAE
                     KADEVARDHRREAAKKAGALIDKLRRRLRDINEAGLTPFAKEELDIVERRLTDVERMV
                     GDGDLAEALGMAHQAKQSLDTIAGELEAAINDDPKSKWADATQDALDGVQRARPVAKE
                     LIDELAALSPRPDQIMSADDHRALDRLRRREAQTRDRAKRLADRAKQL"
BASE COUNT          364 a          869 c          868 g          264 t
ORIGIN      
        1 ggtcgagctc gcgaacgcgc ccccgcgccc cggtgcgcca tcgcgcgcgc tggtcgacgc
       61 cctggtcgag gtcacctcga cccagctggg cacgatcgac ccgcgcagcc tgttcgagcc
      121 cggcgagctg cggcgcgccc ggcgctgggc ggtggtcgcg gtcggggtga acctcgcgtt
      181 cctggtgatc gagccgcgca tggtcgccga tggctggcgc tcgctggtgc tggcgcaccc
      241 ggcgccgttc gacggggcgc ggctgtccgc ggtcccgctg gtcggcgatc tggaggcgac
      301 gctgacgttc ccggcgtact cgcggcggcc gcggctgacc ctgccgtcgt cgtcgggcga
      361 cgtccgcggc ctgcccggaa ccacggtcgc gctgcgcgcc cgcgtgctgg tccccgcggc
      421 cagcgccgag ctgatcatcg agccgatcga ccgcggcgag ccgcgcacga tctcggccca
      481 gctcgacggc gatcagctga ccgcggcgct gacgatcgac cgctccgcgc gctaccggtt
      541 cgcgatcagc tcgccgtcgg gcgagcgcgc gatcgagacc gcgccgcgcg cgatcgaggc
      601 cgagcccgat caggcgccgg cggtccagct catggcgccg gccgatccgc tcgatgtcac
      661 cacgctgcgc cgggtcgagc tcgcctacgt gatcgaggac gacttcggga tcacctcggc
      721 cgagctggtg tgggagtccg gccgcgacaa gggcaagaag ccgatcacgc tggactcgac
      781 ccggggcggc gccgccgcgg cgggagccgg cgctggagcc gggcttggag cctctgggtc
      841 cccggtgccg cgcggccggg tccagggcaa gctgatgtgg gacatcgccg agctccaggt
      901 gccgagcggc ggcgaggtcc ggtactggat cgaggccaag gacaacgaca cggtcggcgg
      961 ccccaacatc ggacggtcac gcgagctgca cctcaaggtg gtgagcccgc gcgagcgcca
     1021 cgaggagacc ctcggccgcc agcaggcgct cgccgagaag atcctgaaga acctcggcgg
     1081 tcggctgacg ctcggcgacg agccggcggc gcgcgacgac ctggcgcgcc agctccgcga
     1141 ggcggtgatc gagctcggca cgatcggcgc ggcgttcgag aaggacccgc acgcgtccga
     1201 tgcgctgcgc aaggcgctgt cgcagatgcg cgagcggctc gacaagctgg cgctcgccga
     1261 gcagcgcctc ttgcccaagg gcaagccgcc ggcccggacc gcgttcgccg cgctcgatcc
     1321 gcggatggtc ggcgagctcg aggacgacac gatcgtgctc gccgactggc tcgaccgcga
     1381 gcgcctggag agcctgctcg acgtgtccga cgagatcgcc gcgcaccaga agcggctcgc
     1441 cgacctgctc gcccagtacc agcgcagcaa ggacccgcgc ctgctcgacg agatcgagcg
     1501 cgagatgcac gcgctcgatc gcgcgtacgc cgagctcgac cgccaccgcc gcggcatggc
     1561 cgaggacgtg ctcgatcagt acgtccaccg gagcgcggtc caggcccagg cgggcacgag
     1621 ctgcatcgac gaggtccgca agctggtgcg cgccggcgaa gcggcccggg cgcagcagaa
     1681 gctcgagcaa tgccgccagc agcaggagcg cgcggcatcg tcgctcgagg gctcgctcgc
     1741 cgggatgcgc ggcgacaagt tcagcgacga gcagaagaag ctcgacgagg tcatgaacga
     1801 gctcgccgac gtcgccaagg agcaggacga catcgccagc gagtccaacc ggatcttcga
     1861 gtcctacgcc gagaaggccg acgaggtcgc ccgcgatcac cgccgcgagg cggccaagaa
     1921 ggccggcgcg ctgatcgaca agctgcgccg ccggctgcgc gacatcaacg aggccgggct
     1981 gaccccgttc gccaaggagg agctcgacat cgtcgagcgc cggctgaccg acgtcgagcg
     2041 catggtcggc gacggcgacc tcgccgaggc gctcggcatg gcgcaccagg ccaagcagag
     2101 cctcgacacg atcgccggcg agctcgaggc cgcgatcaac gacgacccga agtcgaagtg
     2161 ggccgacgcg acccaggacg cgctcgacgg cgtgcagcgc gcccggccgg tcgccaagga
     2221 gctgatcgac gagctcgccg cgctgtcgcc ccggcccgac cagatcatga gcgccgacga
     2281 ccaccgcgcg ctcgaccggc tgcgccgccg cgaggcccag acccgcgacc gcgccaagcg
     2341 gctcgccgat cgcgccaagc agctc
//
LOCUS       DASVOR010002479         5215 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_29116,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002479 DASVOR010000000
VERSION     DASVOR010002479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29116"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VF516_45450"
     CDS             <1..203
                     /locus_tag="VF516_45450"
                     /inference="COORDINATES: protein motif:HMM:NF025189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HEX8115082.1"
                     /translation="GGERAAIAYTILGSCRLAGVDPREYLADVLPRLTGRIRLVDLPA
                     LLPARWAAQRAAAAAAASAAPG"
     gene            complement(665..1120)
                     /locus_tag="VF516_45455"
     CDS             complement(665..1120)
                     /locus_tag="VF516_45455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115083.1"
                     /translation="MQIEIDTEILETIFTQAARAARLAEYLDDPIAVDGTSIIDEVTK
                     SLEKIGRLVAAQLGKPYGLRPEGDPLLDSEPEFDVAVSVDCLRQRVSRVDALATAAER
                     FFEEMPWGGKRQRGRRQLERVAHLVGATIEAVRVAVADGDRLARKLAAR"
     gene            1480..1731
                     /locus_tag="VF516_45460"
     CDS             1480..1731
                     /locus_tag="VF516_45460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115084.1"
                     /translation="MDGFALHELGPLATAIGESWAAELVHSLRSGHRAVVGEWPGTLG
                     EARMRIRVAVRRHIDARLLDELARAANLAARRGWLDVRD"
     gene            1763..2125
                     /locus_tag="VF516_45465"
     CDS             1763..2125
                     /locus_tag="VF516_45465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115085.1"
                     /translation="MVREHEAHHDHVSDGKRDHEREREGHKTDHRADRDPQHDSEHRL
                     DHGAEVFAIAHVQTRASTAQDVLALDQEAEQQAEDAALAPPAEGKVIDRPSRQPTGAI
                     TRQRRYMSGSTSSRFVFA"
     gene            complement(2392..2829)
                     /locus_tag="VF516_45470"
     CDS             complement(2392..2829)
                     /locus_tag="VF516_45470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115086.1"
                     /translation="MSMIETDLQRLEGVWTGTEHVRDGSHDYDASGRLVFQTVFDGRF
                     LLCDYMQTARERPPMFAHGVFRRDDRTSTIAVTWFRTRATAPNQQADAVAEGDTLIFV
                     ETIDGRTTRTSYRIVMDRLSVYTECATGGGEWQRIFEGSYRRR"
     gene            3170..3355
                     /locus_tag="VF516_45475"
     CDS             3170..3355
                     /locus_tag="VF516_45475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115087.1"
                     /translation="MTSIGIKLFSATKARDRENLGETITSWIRMHPEYELLDKIVTQS
                     SDAEFHCLAITVFYRAR"
     gene            3593..3745
                     /locus_tag="VF516_45480"
     CDS             3593..3745
                     /locus_tag="VF516_45480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115088.1"
                     /translation="MATASVGTRDHARDPRVESANDAAESFATALRRLDHKDRHVHIE
                     RGDAVP"
     gene            3742..4176
                     /locus_tag="VF516_45485"
     CDS             3742..4176
                     /locus_tag="VF516_45485"
                     /inference="COORDINATES: protein motif:HMM:NF012534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEX8115089.1"
                     /translation="MTGQVRTTATTLITRDLMDIATLVRILHTSRLARSQLSSGGICR
                     IQSTPGPFRVTQWLALSRVRCGHAVDPMISAGVLRMSEGTIKRLTDRGFGFIATGTGT
                     DMFFHMSAVEGVRFEDLREGQKVSYHEGRGPKGPRAENVRVI"
     gene            4366..>5215
                     /locus_tag="VF516_45490"
     CDS             4366..>5215
                     /locus_tag="VF516_45490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115090.1"
                     /translation="MRALEGEAKQAGLDFAAGRSPRVELAAPSSLALADDPKAPEDPK
                     VAAKDTDPSPEVPDLQNGEVEAIKKAIAAKDRDEALRLMLKALQRIDSTAFKDDDLSE
                     NKLHTGTSKTRQGPKFEAWLKTYLDDVATKKSKTTSKLTKAEVAKAISEAKPPADQKD
                     ILVMIGNGHFGTVSLLYSTVRHEFVHVQQLRKDYLTQISRFVMPAGLDPPDSGKLGED
                     REVEAYLWEMEHLANTGLTDPGELKLLWNACSDAFLNASAGATRTYGARFKAAFKDVW
                     KKAVRSG"
BASE COUNT          928 a         1781 c         1686 g          820 t
ORIGIN      
        1 ctggtggcga gcgcgccgcg atcgcttaca cgatcctcgg ctcgtgccgt cttgcaggtg
       61 tcgacccgcg cgagtacctc gccgacgtcc tgccgcggct gaccgggcgc attcgcctcg
      121 tggacctgcc ggcgctgctc ccagcgcgct gggcagcgca gcgcgccgcc gctgctgctg
      181 ctgcctccgc cgcgcccggc tgagccgtgc gcttgccaca agcgcccgtc gcgctcaagc
      241 ccagccgggt tcggccagat tcggtcagac ggtcacgcgc cgtgttcaca ctgtccatat
      301 aaggaatata cattaccgtc ggcgagaccg tctcggttcg cggcagccca tcgtgctgcc
      361 agccaacccg cacgagttgt cagcacgtcg agcgcatacg atcagcagct gatcgatgcc
      421 gggcgtgtcg gacgcagcca cgcgacgcga tcggcagcgc cgaggtgagt cggacggatc
      481 gtgatgcctc gccacatgtg ctcgctcgtc caccgcgagg agcaggagct tcgcgggtgg
      541 tctccgacga ggattgccgg ctgaatgttc gccgttccat gagaaccgca cccgttcgag
      601 aatgcggaca ccgaaccgcg cgacccagtc tcgcgacgaa cgcggtacgc cgctgcccgc
      661 gcggctaacg cgcagcgagc ttgcgggcga gccggtcgcc gtccgccacc gctacccgca
      721 cggcctcgat ggtcgcgccc accaggtgcg cgacacgctc gagctggcgg cgaccacgct
      781 ggcgcttgcc gccccatggc atctcctcga agaagcgctc tgcagccgtg gccagcgcgt
      841 cgactctcga cacccgctgg cgcaagcagt ccaccgagac cgcgacgtcg aactcgggct
      901 cactatccag gagtggatcc ccctccggtc gcaggccgta cggcttgccg agttgcgctg
      961 cgaccagacg gccgatcttt tcgagcgact tggtaacttc gtcgatgatg ctggtcccgt
     1021 cgacggcgat aggatcgtcg aggtactcgg cgaggcgtgc cgccctcgca gcttgcgtga
     1081 agatggtctc aaggatctcg gtatcgatct cgatctgcat gggtccattg acctccatgc
     1141 cgagacccta cgtcgctggt agacctcgtc gggcttacgg gcaatgttgc cttgcgtcct
     1201 tcgccggacg gtcacccgcc ggccgcggat gtgcacgccg aagaccttgc agcgcgacgc
     1261 gacaacgaca cgatggaatt cgcatccatg ttgtcccgtg tggacgcgac gcgtcgcgtc
     1321 tgcggtgcta cgctctgtcc ggatcgaagc tcggagcccc acggataggc cgtggccttt
     1381 tggcggcgtc caggcgagac catgacgaac cgaatgggcg ggcatcccga acgccgccac
     1441 cacgcgcgca tctcccccaa gggctcatcg ctcctccgct tggacggctt cgcgctgcac
     1501 gagctcgggc cgctggcgac ggcgatcggc gagtcgtggg ccgccgagct cgtgcactcg
     1561 ctgcgctcag gtcatcgtgc cgtcgtcggc gaatggccgg gcaccctggg cgaggcgcgc
     1621 atgcgaatcc gcgtcgcggt ccggaggcac atcgatgctc gactcctcga cgaactcgcg
     1681 cgcgcggcga acctcgcggc gcgccgcggc tggctcgatg tccgggactg acccaggatc
     1741 gctcggtcgc cgggctcagg ccgtggttcg tgagcatgaa gcccatcacg atcacgtcag
     1801 cgacggcaag cgggatcacg aacgcgaacg tgaaggccac aagaccgatc atcgcgccga
     1861 ccgcgatcca cagcacgaca gcgagcatcg tctcgatcac ggcgcggagg tattcgcgat
     1921 cgctcatgtt cagacgcgtg cgtccactgc gcaggatgtc cttgctctgg accaagaagc
     1981 cgagcagcaa gctgaggatg ccgcgcttgc gccgcccgcc gaaggcaagg tgatcgatcg
     2041 accatcgcgt caaccgactg gcgcgataac gcgccagcga cgatacatgt cggggtcgac
     2101 gtcaagtcgg ttcgtgttcg cgtgatgcac gcggttgtgc cacaccaccc agagctgttg
     2161 cgccagcgcg aagggggcaa aacacagcca gccgacgatg agtcgtagcc agttccatcg
     2221 accgcgcacg actgcgccgt gaagcacctc gtgtccgagg aacatgagcc cggcaaagct
     2281 catcccgatc aggacggaga cgaggccaac gcatggccac ggaaaccagc gttccgcgat
     2341 cgccatcata ccaccacgat caccgacacg tggatcggca accagccgag cctagcggcg
     2401 gcggtacgag ccttcgaaga tccgctgcca ctcgccgccg ccggtcgcgc attccgtata
     2461 cacggacagc cgatccatca cgatgcgata gctcgtgcgc gtcgtgcgac cgtcgatagt
     2521 ctcgacgaag atcaacgtgt caccctcggc gaccgcgtcg gcctgctggt tcggcgccgt
     2581 ggcgcgggtt cgaaaccagg tgacggcgat cgtgctcgtg cgatcgtctc gccggaacac
     2641 accgtgagcg aacatcggcg gtcgttcgcg tgcggtctgc atgtagtcgc aaagcagaaa
     2701 gcgtccatcg aacacggtct ggaatacgag tcgccccgac gcgtcgtagt catggctgcc
     2761 atcgcgaacg tgctcggtgc cggtccacac accttcgagt cgctgcaagt ccgtctcgat
     2821 catgctcatg gtgtcgccct tgtctcgcag atctcggtcg ccacacacac gccacccgcg
     2881 gcgatgctca ccagccgccc gcgatgcacg ttcccgagcg catggagcgt gaccgcacat
     2941 ttcggggcga tgcgtgcgtg cgggcggcga tcgggctcgc gcccggagcg ttgggtcatg
     3001 atctctcagc ctggacgccg cacaaaacga accacggcca atccgtggcg gccccgagct
     3061 tcgatccgag accaccatag cacaccgttc ggcgggcgcc cgcacgcgtc gctgcgcacc
     3121 gcgcgtcgag tcgtgaacca ggcgggacat cgcgtgctac ggtgctgtct tgaccagcat
     3181 aggcatcaag ttgttctcgg cgacgaaggc gcgcgaccgc gagaacctgg gggaaacgat
     3241 caccagctgg atccgcatgc acccggaata cgagctctta gacaagatcg tgacccagtc
     3301 ctccgatgct gagttccatt gtctcgcaat caccgtgttc taccgagcac gctgatcgcc
     3361 tcgaacggcc gcaggcgatc ggggcgtgct ccgctgcggc tcggcttcat cgaagctgat
     3421 ccggccgcgg cgcgcggccg aaacgtgctc tcggcgggag cccacaggtc ctcgtgcatc
     3481 gcagcggtca caggggacgg gcgggcaagc acagcatcgc gatcctgctc gtccccgaac
     3541 gcgcacggcg tcacatcgag cggttgatcg ccgcggcgca cgtcgaggtc tcgtggcgac
     3601 cgcctccgtc ggcacacgag atcacgcccg cgacccgcgc gtcgagagcg cgaacgacgc
     3661 cgccgagagc tttgcgaccg cgctacgacg cctcgaccac aaggaccgcc acgtgcacat
     3721 cgagcgtggt gacgcggtgc cgtgaccggc caggtgcgca cgaccgcgac gacgctgatc
     3781 acgcgggatc tcatggacat cgcgacgctg gtgcgcatcc tccatacgtc cagacttgcg
     3841 cggtcgcagc tgtcatcggg cggaatatgc agaattcagt cgacaccggg cccatttcgc
     3901 gtcacacagt ggctggctct gtctcgtgtc cgctgtggtc acgcggtcga tccaatgatc
     3961 tctgctggag tgctacgaat gtctgaaggt acgatcaagc gtctgactga taggggtttt
     4021 ggtttcatcg cgactggcac aggcaccgat atgtttttcc acatgtcggc tgtcgagggc
     4081 gttcgcttcg aggatctgcg cgaagggcag aaggtctcgt atcacgaagg gcgcggcccc
     4141 aagggtccgc gcgccgagaa cgtgcgcgtg atctgaacaa ctcacaatga caaaaccggg
     4201 gaatcgagcc ggcctgaccg ccggctcgat ttcgttgtca ccgtcgcggg caaggtcgac
     4261 ttcgcgcccg gcaagttcga cctcgcgagc cacggcggcc gcgcgcggct cggcgaggag
     4321 accgcccacg cggtccagca gcgcaaccag ggcgaggcgt cgagcgtgcg cgcgctcgag
     4381 ggcgaggcca agcaggccgg gctcgacttc gcggccggcc ggagcccgcg ggtcgagctt
     4441 gccgcgccgt cgagcctcgc gctcgccgac gatcccaagg cccccgagga tcccaaggtg
     4501 gccgccaagg acaccgatcc atcgccggag gtccccgacc tccagaacgg cgaggtcgag
     4561 gcgatcaaga aggcgatcgc cgccaaggac agggacgagg cgctgcgcct gatgctcaag
     4621 gcgctgcagc gcatcgactc gacggcgttc aaggacgatg acctgtccga gaacaagctg
     4681 cacaccggta cgtcgaagac ccgccagggc ccgaagttcg aggcctggct caagacctac
     4741 ctcgatgacg tcgccaccaa gaagtccaag acgaccagca agctgaccaa ggccgaggtc
     4801 gccaaggcga tcagcgaggc caagccgccc gccgatcaga aggatatcct ggtcatgatc
     4861 ggcaacggcc acttcggcac ggtctcgctg ctgtactcga cggtgcgcca cgagttcgtc
     4921 cacgtccagc agctgcgcaa ggattacctg acccagatct cgcgcttcgt gatgccggcc
     4981 ggcctcgatc cgccggactc cggcaagctc ggggaggacc gcgaggtcga ggcctacctc
     5041 tgggagatgg agcacctcgc gaacacgggc ctcacggatc cgggcgagct caagctgctg
     5101 tggaacgcgt gcagcgacgc gttcctcaac gccagcgccg gcgcgaccag gacctacggt
     5161 gcgcggttca aggccgcgtt caaggacgtg tggaagaaag cggttcgatc cggat
//
LOCUS       DASVOR010002480        10064 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_29129,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002480 DASVOR010000000
VERSION     DASVOR010002480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10064
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29129"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VF516_45495"
     CDS             complement(<1..857)
                     /locus_tag="VF516_45495"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8115091.1"
                     /translation="MVSSQVDVPPAPDHDAPSGPLQAGHLISAYRIEKLVGVGGMGWV
                     YRARHELNARSVALKILREDQLAQDRAIDRVMREATILASVPHPGLPRFHECGVLDDG
                     RPWIAMELVEGVALAHRMQGSPLDADAVIDFVVLVAEVLAAVHAREFTHRDLKPDNII
                     LAPGDARFPIRVIDWGIALAGAGARFTNMNEAIGTPTYMAPEQARGSKTEERSDVYGL
                     GVVAYQALTGRAPFVGSSAVEILVQHLNRPPPALAPRCPDAPYGLVELVERMLAKDPE
                     ERPKAAEISA"
     gene            1008..2159
                     /locus_tag="VF516_45500"
     CDS             1008..2159
                     /locus_tag="VF516_45500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8115092.1"
                     /translation="MTSEPLAELGRDSLVGAYRIVREIGRGGMATVYEARHTVLPRRA
                     ALKVMHGDLRRQPGMATRIVQEAAILDSVRHPGIVRVYDCSVLPDHRPWIAMELVEGE
                     TLANRLHDLTALAPVEVATLLGDVADVLAAVHKVGVVHRDLKPDNLLCTPTDYDYPIR
                     VLDWGVARMGPIGRLTIDGLTPGTPIYMSPEQATGRDVGAPCDIYSLGVIAYEALTGR
                     PPFDGHTLAEVVCMHLTRAPAPLRARCGAPAELCNLVGRMLHKEPTARPTAAEVRQAA
                     RAIARELSQAYEEFELSGIEIDRTVTRPRSQPAYLVPSDEVVVVDVDATELEYGVTEM
                     LPVVRQPRWTPEISGMPSVLAEPSPRPAVAPRAPRDQVAGEIIATHKSR"
     gene            2402..2677
                     /locus_tag="VF516_45505"
     CDS             2402..2677
                     /locus_tag="VF516_45505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115093.1"
                     /translation="MNKNGMTDVGSSTRTVDERLDSIKSTVRDVVDQGAQKADALKSR
                     VIEAKDQALRRGNDLLDRVVELIKAHPLKAVGIAFATGYFGMRLFRR"
     gene            2686..3123
                     /locus_tag="VF516_45510"
     CDS             2686..3123
                     /locus_tag="VF516_45510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115094.1"
                     /translation="MASNTTDPVVVEADRRVEQAKASLRARFAVLERRLGDVRDRIDL
                     PEQIRRHPLPAVGIAFALGALVGGRGHRPAGHAAPAPLAFEARPRTLGSMALAVLSGL
                     TFQLLRELAIGRLRRVAEQWWGEQGGAFDEASSAHDLAPHTEP"
     gene            3156..3329
                     /locus_tag="VF516_45515"
     CDS             3156..3329
                     /locus_tag="VF516_45515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115095.1"
                     /translation="MKFFVKAVVTGFALSLGSALFKKVQRHLGLGDDSEKTAPEAVKQ
                     DGATDPSLQQRFS"
     gene            3467..4030
                     /locus_tag="VF516_45520"
     CDS             3467..4030
                     /locus_tag="VF516_45520"
                     /inference="COORDINATES: protein motif:HMM:NF014659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEX8115096.1"
                     /translation="MESSNLAPGLLLAMPQLTDPNFSRSVVLMIEHGEQGSFGLVINH
                     PSPIRAAELLDSLEMSWRGEASAVVWAGGPVSPSTGWVLHEPISIAEPGAGGTIEVTS
                     SISLSMSPERLRVLASQPPRNIRLLLGYSSWAPGQLANEMARGAWLHTSVDPDLVFET
                     PADEIWDTAMRSLGINPRDLFTGRGVN"
     gene            complement(4151..5470)
                     /locus_tag="VF516_45525"
     CDS             complement(4151..5470)
                     /locus_tag="VF516_45525"
                     /inference="COORDINATES: protein motif:HMM:NF019606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEX8115097.1"
                     /translation="MALKKGVDVKPAKGKLGILLPGMGAVATTFIAGVQAIRRGIGKP
                     IGSLTQLGHIRIGKRTDDNSPAIKDYVSLATLDDIVFGGWDIFPDNAYQAAVRAGVLD
                     ARHLDPLKDELEAIRPMPAVFEQAYVKKLSGPNVKKGATKMHLAEQVMDDIRAFKAHH
                     GCDRLVAVWCGSTEVYRKPAEVHSTLDRFEAALRGSHDDIAPSQIYAYACLKMGVPYA
                     NGAPNLSVDTPALLELARRQGLPVAGKDFKTGQTLMKTILAPGFKARMLGLSGWYSTN
                     ILGNRDGEVLDDPESFKSKEVTKLGVIDHILQPQLYPELYGNVSHVVRINYYPPRGDN
                     KEGWDNIDIFGWLGYPMQIKVNFLCRDSILAAPIVLDLALFMDFAQRAGMSGIQEWLS
                     FYWKSPMTPEGLYPEHDLFIQLMKLKNTLRYTKGDELITHLGAEYYD"
     gene            complement(5729..6490)
                     /locus_tag="VF516_45530"
     CDS             complement(5729..6490)
                     /locus_tag="VF516_45530"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HEX8115098.1"
                     /translation="MHPRRLYAHRGASAERPENTLAAFERAVEVGVDALEMDVHVTRD
                     NHLIVAHDDTAARTCGARLAWADLDLADVGRLDAGWGFVAPDGTRPFAARAVRVPTFE
                     QVLDAFPAMRINVDLKGDRAAALMLDLLHRKRAEDRVTLASFQLGTLVEIRRRGYGGE
                     TALSQPEVAALLALPAMLWRQLPFTGTAAQVPVHAGRIRFDRATFIAKCHSIGLRVDF
                     WTVDDPDEAARLLALGADGIMTNHPRAVRAIVQRS"
     gene            complement(6490..6957)
                     /locus_tag="VF516_45535"
     CDS             complement(6490..6957)
                     /locus_tag="VF516_45535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115099.1"
                     /translation="MKIENGRRVTLKVELSVVGGATLEKSTVEYIHGGGTMLPGLESV
                     LAGLEKGARREGVLKAKDAFGNPSMHPLKKMKRSDFPKDAKLEVGEKLVAKGANDMNV
                     VLRIEKVSGDDVDVRLVHPLAEKDIRYSAEVIQVSDPRPPPVPAQALELEEDS"
     gene            7226..7534
                     /locus_tag="VF516_45540"
     CDS             7226..7534
                     /locus_tag="VF516_45540"
                     /inference="COORDINATES: protein motif:HMM:NF015147.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HEX8115100.1"
                     /translation="MLGVQQEPRHDVVFVQVTNPASHAMRLTRLEYTFAAAGQTVSAG
                     EVALARDVPAGEAVVVQIPLDSPPERPMTLSGKLTAELDQIVQIFSVSAQVAPPTAAQ
                     "
     gene            7624..8397
                     /locus_tag="VF516_45545"
     CDS             7624..8397
                     /locus_tag="VF516_45545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115101.1"
                     /translation="MPRSVLALLVLALLAVPAAAGLKQVRYVGIHPLPKSEGGGICYI
                     EGPHIHVFDADKIQYRDHRGANYFVGDPVAYGYDGPRYAYKGHHPIHVAAVIGDDDPG
                     DVYCYINGPHYHYFAPPEGPEFKVVGGAYFYVADPPRAYVEARPAMMRINTVYQPLVY
                     ERPVITVEAPVGWIGARAEFVGPAAVVAAPGAVVVAPAAGISVEAHVPMPSLHVDVGI
                     GAPAVIVHDRPAVIVHDRPVIIEERVKVKHDNGLHRGHH"
     gene            8427..8780
                     /locus_tag="VF516_45550"
     CDS             8427..8780
                     /locus_tag="VF516_45550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115102.1"
                     /translation="MLALAACGKGGDKPAGPRDAVIAAWKADKLTPSALAPATVAFGK
                     DCQSGTVENLDVLVCSFASPAEAKAAEDPGLTWIGSATGASQAHGSALIVLADRKKAD
                     PNGRMINRLMKLAPN"
     gene            8803..9861
                     /gene="bioB"
                     /locus_tag="VF516_45555"
     CDS             8803..9861
                     /gene="bioB"
                     /locus_tag="VF516_45555"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEX8115103.1"
                     /translation="MSNPIWSALADTALAGAPITRDRARAVLEAPPDELLGLLDAAFR
                     VRRAHWGRRVSLHVLENAKLGACPEDCGFCSQSQRHASPGGEAPIKTVDELVAGARRA
                     HAARAKRYCMVTATRGPSQRDLDVICAAASQIKAEMDIELCASLGLLTEAKAERLASA
                     GIDRFNHNLETSERHYGAIVTTHTWRDRVETVRIAQRAGMDTCCGGIVGLGETLDDLL
                     DLAFALRELDVDSVPVNFLDARPGTPLAGSPRVEPGFALRALCMFRFVHPRTDLRVAG
                     GREITLRALQVMALYPANSIFTQGYLTTAGATPHDDHQMIRDAGFELELASGEAVPAD
                     PEAVAHLARRPMLPVARG"
     gene            complement(9880..>10064)
                     /locus_tag="VF516_45560"
     CDS             complement(9880..>10064)
                     /locus_tag="VF516_45560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115104.1"
                     /translation="TSTVPVLRASTERDNGRGRRWGQTSTYRFAAVKLNAGVKVDVIV
                     DVDVERHGNDHEREDL"
BASE COUNT         1478 a         3506 c         3495 g         1585 t
ORIGIN      
        1 gcgctgatct cggcggcctt gggccgctcc tcggggtcct tggcgagcat gcgctcgacg
       61 agctcgacca ggccgtacgg cgcgtccggg cagcgcggcg cgagcgccgg cggcgggcgg
      121 ttgaggtgct ggacgaggat ctcgaccgcg ctcgatccga cgaacggcgc ccgccccgtg
      181 agcgcctggt aggccaccac gccgagcccg tagacgtcgg agcgctcctc ggtcttgctg
      241 ccgcgcgcct gctcgggcgc catgtacgtc ggcgtcccga tcgcctcgtt catgttggtg
      301 aaccgcgcgc ccgcaccggc gagcgcgatc ccccagtcga tcacgcggat cgggaaccgc
      361 gcgtcgccgg gcgcgaggat gatgttgtcg ggcttgagat cgcggtgggt gaactcgcgc
      421 gcgtggaccg cggcgagcac ctcggcgacc agcaccacga agtcgatcac cgcgtcggcg
      481 tcgagcgggc tgccctgcat gcggtgagcg agcgcgacgc cctcgaccag ctccatcgcg
      541 atccacggcc ggccatcgtc gagcaccccg cactcgtgga accgcggcag gcccgggtgc
      601 gggaccgacg cgaggatcgt cgcctcgcgc atcacgcggt cgatcgcgcg atcctgtgcg
      661 agctgatcct cgcgcaggat cttgagcgcc acggatcgcg cgttcagctc gtgacgggcg
      721 cgatacaccc agcccatccc gccgaccccc acgagcttct cgatccggta cgccgagatc
      781 aggtggccgg cctggagcgg gccggatgga gcgtcatggt ccggagcggg cgggacgtcg
      841 acctgcgagc tgaccatgac ggagccaccg ggagcacacc acgtgccagc acagctcgct
      901 gcgatctcgc tttgtgcgct ggtgctacac gctgtgcaac ctggccttga atcgactgga
      961 tcttggatag caaatctcct ggctaggttc gtgcacccgc aacccacatg acctccgagc
     1021 ccctcgccga acttggtcga gactccttgg tgggtgcgta tcggatcgtg agggagatag
     1081 gtcggggtgg aatggccaca gtatacgaag ccaggcacac cgtcctgccg cgtcgtgcgg
     1141 cactcaaggt catgcatggg gatctacggc gccagccggg gatggccacg cgcatcgtgc
     1201 aagaagcggc catcctggac agcgttcgcc atcccgggat cgtccgggtc tacgactgca
     1261 gcgtgctccc ggaccaccgc ccgtggatcg cgatggagct cgtcgagggc gagaccctgg
     1321 cgaaccggct ccacgacctc accgcgctgg ccccggtcga ggtcgccacg ctgctcggtg
     1381 acgtcgccga cgtgctggct gcggttcaca aggtcggtgt cgtacatcgt gatctgaagc
     1441 ccgataacct gctctgcacg cccacggatt acgattaccc gattcgcgtg ctcgactggg
     1501 gcgtcgcccg catggggccg atcggccggc tgaccatcga tggcttgacg ccgggaacgc
     1561 cgatctacat gtcccccgag caggcgaccg gccgcgacgt cggcgcgccg tgcgacatct
     1621 actcgctcgg ggtcatcgcg tacgaggcgt tgaccggccg accgccgttc gatggccaca
     1681 ccctcgccga ggtggtctgt atgcacctca cccgcgcgcc ggcgccgctg cgcgcgcgct
     1741 gcggtgcgcc tgccgagcta tgcaacctgg tcggccgcat gctccacaag gagcccacgg
     1801 cgcggcccac ggcggccgag gtccggcagg ccgcgcgcgc gatcgcccgc gagctgtcgc
     1861 aggcgtacga ggagttcgag ctgtccggca tcgagatcga ccgcaccgtg acgcggcctc
     1921 gcagccagcc ggcgtacctc gtgcccagcg acgaggtcgt cgtggtcgat gtcgacgcca
     1981 ccgagctcga gtacggcgtc accgagatgc tgcccgtggt ccgccagccg cgctggaccc
     2041 cggagatcag cgggatgccg agcgtgctcg ccgagccatc gccccgaccc gcggtcgcgc
     2101 cgcgtgcccc gcgcgatcag gtcgccggcg agatcatcgc gacccacaag tctcgatgac
     2161 gccggcacag cctccgtgcg ctcccgtcac actgcgcatt ttcgcaacgt gttcgttgtg
     2221 agtcacgggt gagaaatctg gtcatagccc acgaaatttt ggcaccgcag cgaaccacgt
     2281 agcgcgccga gtcaccaacg aatccgaata gttgagtgga ctcgttccat cgattcagac
     2341 tcgtttcctg aataccccat tctgctcgat tgttgaactt ccttcgtcag gagaagtcac
     2401 catgaacaag aacggcatga ccgatgtagg ctcgtcgacc cgcacggtcg acgagcggct
     2461 cgactcgatc aagagcaccg tgcgggacgt cgtcgaccag ggcgcccaga aggccgacgc
     2521 gctcaagtcc cgcgtgatcg aggccaagga ccaggcgctc cgccgcggca acgacctgct
     2581 cgaccgcgtc gtcgagctga tcaaggcgca ccccctcaag gccgtcggca tcgcgttcgc
     2641 caccggctac ttcggcatgc ggctgttccg gcgctgagcc acgtcatggc gagcaacacc
     2701 acggaccccg tcgtcgtcga ggccgatcgg cgcgtcgagc aggcgaaggc ttcgctgcgc
     2761 gcgcggtttg cggtcctcga gcgccggctt ggcgacgtgc gcgatcggat cgatctgccc
     2821 gagcagatcc ggcgacaccc gttgccggcg gtcggcatcg cgttcgcgct cggcgcgctc
     2881 gtcggtggcc gcggtcaccg ccccgccggc catgccgcgc cggccccact ggcgttcgag
     2941 gccaggccgc gcacgctcgg cagcatggcg ctggcggtgc tgtccggact gaccttccag
     3001 ctcctgcgcg agctcgcgat cggtcggctc cggcgcgtcg ccgagcagtg gtggggcgag
     3061 caaggtggag cgttcgacga ggcttcgtcg gcgcacgacc tcgctcccca caccgagccg
     3121 tgatcggcgc ggcgcacgat gctacgatcc ccggcatgaa gttcttcgtg aaagcggtcg
     3181 tcaccgggtt cgccctgagt ctgggttcgg cgctgttcaa gaaggtgcag cgccacctcg
     3241 ggctgggcga tgacagcgag aagaccgctc ccgaagcggt caagcaggac ggcgcgaccg
     3301 atccgagcct gcagcagcgg ttcagctgat cggccgaaat cgtcgggcct gcctcgcccc
     3361 cggagccagt ggtctgaccg accgcgcgac cggcccccgg gcgggagcag catttgtcat
     3421 cgattccagc ggcgatcggc cggaaagtgc ggaacactgg aggcaggtgg agtcctcgaa
     3481 cctcgccccg ggcctgttgc ttgcgatgcc gcagctcacg gatccgaatt tctcgcgctc
     3541 ggtcgtcctc atgatcgagc acggcgagca aggttcgttc ggcctggtca tcaatcaccc
     3601 gtcgccgatc cgcgccgccg agctgctcga ctcgctcgag atgagctggc gcggcgaggc
     3661 ctccgcggtc gtgtgggccg gcggcccggt atcgccgagc accggctggg tgctccacga
     3721 gccgatcagc atcgcggagc cgggcgccgg cgggacgatc gaggtcacct cgtcgatctc
     3781 gctgtcgatg tcccccgagc ggctgcgcgt gctcgccagc cagccgccgc gcaacatccg
     3841 cctcttgctc ggctactcga gctgggcccc gggccagctc gcgaacgaga tggcgcgcgg
     3901 tgcgtggctg cacaccagcg tcgaccccga cctcgtgttc gagactcccg ccgacgagat
     3961 ctgggacacc gcgatgcgct cgctcggcat caacccgcgc gacctgttca ccggccgcgg
     4021 cgtgaactag aaaaaccggc tgcgggcccc gggcgcttcg ttgtccgtcc gcttccggtc
     4081 ctggcgtacg tgagtacgct ccggtccggt tctccctccg cctcgcgcgc ggggctcctc
     4141 gctcggtttt ttaatcatag tactcggcgc cgaggtgggt gatcagctcg tcgcccttgg
     4201 tgtagcgcag cgtgttcttc agcttcatga gctggatgaa caggtcgtgc tcggggtaca
     4261 ggccctcggg ggtcatcggc gacttccagt agaacgacag ccactcctgg atgccgctca
     4321 tgccggcgcg ctgggcgaag tccatgaaca gcgcgagatc gaggacgatc ggcgccgcga
     4381 ggatcgagtc gcggcacagg aagttgacct tgatctgcat cgggtagccg agccacccga
     4441 agatgtcgat gttgtcccag ccttccttgt tgtcgccgcg cggcgggtag tagttgatgc
     4501 ggacgacgtg ggacacgttg ccgtagagct cggggtagag ctggggctgc aggatgtggt
     4561 cgatcacacc gagcttggtg acctccttgc tcttgaacga ctcggggtcg tcgagcacct
     4621 cgccgtcgcg gttgcccagg atgttggtcg agtaccagcc cgacagcccg agcatgcgcg
     4681 ccttgaagcc cggcgcgagg atcgtcttca tcagggtctg gccggtcttg aagtccttgc
     4741 cggcgaccgg caggccctgc cggcgggcga gctcgagcag cgccggggtg tcgaccgaca
     4801 ggttgggggc gccgttggcg tacggcacgc ccatcttgag gcaggcgtag gcgtagatct
     4861 gcgacggcgc gatgtcgtcg tgcgagccgc gcagggcggc ctcgaagcgg tcgagcgtgc
     4921 tgtggacctc ggcgggcttg cggtagacct cggtcgagcc gcaccacacg gcgaccaggc
     4981 gatcgcagcc gtggtgggcc ttgaacgcgc ggatgtcgtc catcacctgc tcggcgaggt
     5041 gcatcttggt cgcgcccttc ttgacgttgg ggccgctcag cttcttgacg taggcctgct
     5101 cgaacacggc cggcatcggc cggatcgcct cgagctcgtc cttgagcgga tcgaggtggc
     5161 gcgcgtcgag cacgccggcg cggaccgcgg cctggtaggc gttgtcgggg aagatgtccc
     5221 agccgccgaa cacgatgtcg tcgagcgtgg cgagcgagac gtagtccttg atcgccggcg
     5281 agttgtcgtc ggtgcgcttg ccgatgcgga tgtggccgag ctgggtcagc gagccgatcg
     5341 gcttgccgat gccgcggcgg atcgcctgca cgccggcgat gaacgtggtg gcgaccgcgc
     5401 ccatgccggg cagcaggatg ccgagcttgc ccttggccgg cttgacgtcg acgcctttct
     5461 tgagtgccat gacctgctcc gatcgagttg gaagtctgga aattgctccg gaaccgatca
     5521 cctttcaagg gccacagagc ggctggtggc ggcaaggcac agtgtcgaat cttcaacacg
     5581 cgccggcgcg catgcgtgcg cccgtcgacg cggccgcgcg cccggggccg cagcgaccgt
     5641 gcggaaagtc atcgcggcgg acaccttctg gacaacctcg ggcgaaatcg tagaacggac
     5701 cgcgcccgag cgggcaccac ccggaacctc acgaccgctg gacgatcgcc cggacggcgc
     5761 gcgggtggtt ggtcatgatg ccgtcggcgc cgagcgccag gagccgcgcc gcctcgtcgg
     5821 gatcgtcgac cgtccagaag tcgacgcgca ggccgatgct gtggcacttg gcgatgaacg
     5881 tcgcgcgatc gaaccggatc cggccggcgt ggaccgggac ctgcgccgcg gtgccggtga
     5941 acggcagctg gcgccacagc atcgccggca gggcgagcag ggccgcgacc tcgggctgcg
     6001 agagcgcggt ctcaccgccg taaccgcggc ggcggatctc gaccagcgtg ccgagctgga
     6061 agctcgccag ggtgacgcgg tcctcggcgc gcttgcggtg gagcaggtcg agcatgagcg
     6121 cggctgcgcg gtcgcccttg aggtcgacgt tgatccgcat cgcggggaac gcgtcgagca
     6181 cctgctcgaa cgtcggcacg cggaccgccc gggcggcgaa cggccgggtg ccgtcggggg
     6241 cgacgaaccc ccagccggcg tcgagccggc cgacgtcggc gaggtcgagg tcggcccagg
     6301 cgagccgtgc gccgcaggtc cgcgccgcgg tgtcgtcgtg cgcgacgatc aggtggttgt
     6361 cgcgggtgac gtgaacatcc atctccagcg cgtcgacccc gacctcgacc gcgcgctcga
     6421 acgcggccag ggtgttctcg gggcgctcgg ccgacgcgcc gcggtgggcg tacagccgac
     6481 gcgggtgcat cacgagtctt cttcgagctc gagcgcttgc gccggcaccg gcggcggccg
     6541 cggatccgag acctggatca cttcggccga gtaccggatg tccttctcgg cgagcgggtg
     6601 gaccagccgg acgtcgacgt cgtcgcccga gaccttctcg atccgcagca cgacgttcat
     6661 gtcgttggcc cccttggcga ccagcttctc gccgacctcg agcttggcgt ccttggggaa
     6721 gtcggaccgc ttcatcttct tgagcggatg catcgacggg ttgccgaacg cgtccttggc
     6781 cttgaggacg ccctcgcgcc gggcgccctt ctccagcccg gcgagcacgc tctccaggcc
     6841 gggcagcatg gtacctccgc cgtggatgta ctcgacggtg ctcttctcga gggtcgcacc
     6901 tccgacgacg gatagctcga ccttcagggt gacccggcgg ccgttctcga tcttcacccg
     6961 gcgacggtac acgtgagcgc tccgcgacag caacgtccgg ggctgtgaac gcagggtggc
     7021 gaacgttcgc ggcgccgatg tggttgcaat aagttgcgcg ctggcatgga agccgcacac
     7081 tcgacgtcct cggtcgatca tgcgtcgctc ggttcccgcc ttccgcttcg gtcgtggtct
     7141 cgcgctctgc ctcggtgtgt ggctctggtt cgccggcgcc ggccttgcga cgggctgccg
     7201 cagcggacct tcacccgagc ttcatgtgct cggcgtccag caggagccgc ggcacgacgt
     7261 ggtgttcgtc caggttacca acccggcgag ccacgcgatg cggctgaccc ggctggagta
     7321 cacgttcgcg gccgccggac agaccgtgtc cgccggcgag gtggcgctgg cgcgcgatgt
     7381 cccggccggc gaggccgtgg tggtccagat cccgctcgac agcccacccg agcggccgat
     7441 gacgctgtcc ggaaagctca ccgccgagct cgaccagatc gtgcagatct tctcggtgtc
     7501 cgcgcaggtc gcaccgccga cggcggctca gtagcgccca caagacctcg aaatcgctcg
     7561 aggcgcacgt gcgcacacac cgattcttgt aaccttaccc gacgttgcgc cacaccttgg
     7621 ggcatgccca gaagcgtcct tgctctgctc gtgctcgctc tgctcgccgt gccggcggcc
     7681 gccggcctca agcaggtccg atacgtcggc atccacccgc tcccgaagtc ggagggtgga
     7741 ggcatctgct acatcgaagg cccgcacatc cacgtattcg acgccgacaa gatccagtat
     7801 cgcgatcacc gcggcgcgaa ctacttcgtg ggggacccgg tcgcctacgg ctacgacggc
     7861 ccgcgctacg cctacaaggg ccatcacccg atccacgtcg ccgcggtgat cggcgatgac
     7921 gacccgggcg acgtgtactg ctacatcaat ggcccgcatt accattattt cgcgccgccc
     7981 gaggggcccg agttcaaggt ggtcggcggg gcgtacttct acgtcgccga tccaccgcgc
     8041 gcctacgtcg aggcccggcc ggcgatgatg aggatcaaca cggtgtacca gccgctggtc
     8101 tacgagcgcc cggtgatcac cgtcgaagcc ccggtcggct ggatcggggc gcgcgccgag
     8161 ttcgtcggcc ccgccgcggt ggtcgccgcg cccggggcgg tcgtggtcgc ccccgccgcc
     8221 gggatcagcg tcgaggccca cgtgccgatg ccgagcctgc acgtcgacgt cgggatcggc
     8281 gcgcccgcgg tgatcgtcca cgaccgcccc gccgtgatcg tccacgatcg cccggtgatc
     8341 atcgaggaac gcgtcaaggt caagcacgac aacggcctgc accggggtca ccactgaggg
     8401 cggcgatgcg caggatcatg atgctgatgc tcgcgctcgc cgcgtgcggc aagggcggtg
     8461 acaagcccgc cggcccgcgc gatgccgtga tcgcggcctg gaaggccgac aagctgaccc
     8521 cgtcggcgct ggccccggcc acggtcgcgt tcgggaaaga ctgccagagc ggcacggtcg
     8581 agaacctcga cgtgctggtg tgcagcttcg cgtcgccggc cgaggccaag gcggccgagg
     8641 atccggggct gacctggatc ggctcggcca ccggcgcgtc gcaggcccac ggttcggcgc
     8701 tgatcgtgct ggcggatcgc aagaaggccg atccgaacgg ccggatgatc aaccggctga
     8761 tgaagctggc gccgaactag cgcattcgct agcgtgccgg ccatgtcgaa cccgatctgg
     8821 tcggcgctgg ccgacaccgc gctcgccggc gcgcccatca cccgcgaccg ggcgcgcgcc
     8881 gtgctggagg cgccgcccga cgagctgctc gggctgctcg acgccgcgtt ccgggtccgc
     8941 cgcgcgcact ggggccggcg ggtgtcgctg cacgtgctgg agaacgccaa gctcggcgcc
     9001 tgcccggagg actgcgggtt ctgctcgcag tcgcagaggc acgcgtcgcc gggcggcgag
     9061 gcgccgatca agacggtcga cgagctggtc gccggggcgc gccgggcgca cgccgcgcgg
     9121 gccaagcgct actgcatggt gaccgcgacc cgcgggccat cgcagcggga tctcgacgtg
     9181 atctgcgcgg ccgcgtcgca gatcaaggcc gagatggaca tcgagctgtg cgcgtcgctc
     9241 ggccttctga ccgaggccaa ggccgagcgg ctggcgagcg ccgggatcga ccgcttcaac
     9301 cacaacctcg agaccagcga gcgccactac ggcgcgatcg tcaccaccca cacctggcgc
     9361 gaccgggtcg agaccgtgcg gatcgcgcag cgcgcgggga tggacacctg ctgcggcggc
     9421 atcgtcgggc tcggcgagac gctcgacgac ctgctcgacc tcgcgttcgc gctgcgcgag
     9481 ctcgacgtcg actcggtgcc ggtcaacttc ctcgacgccc ggccgggcac gccgctggcg
     9541 ggctctccac gggtcgagcc cggcttcgcg ctgcgcgcgc tgtgcatgtt ccggttcgtc
     9601 cacccgcgca ccgacctgcg ggtcgccggc gggcgcgaga tcacgctgcg cgcgctgcag
     9661 gtcatggcgc tctaccccgc gaactcgatc ttcacgcagg gctacctgac caccgcgggt
     9721 gccacgccgc acgacgatca ccagatgatc cgcgacgccg ggttcgagct cgagctcgcg
     9781 tccggcgagg ccgtgccggc cgatcccgag gcggtcgccc accttgcgcg ccggccgatg
     9841 ctgccggtcg cgcgcggcta gcccgactct gatgattcgt cacaggtcct cgcgctcgtg
     9901 gtcgttcccg tggcgctcga cgtcgacatc gacgatgacg tcgaccttga ctccagcgtt
     9961 gagcttgacg gcagcgaagc gatacgtgga cgtctgaccc caccgtcgac ctcgcccgtt
    10021 gtcgcgctcc gtcgaggctc gcagcacagg aacggtcgac gtcc
//
LOCUS       DASVOR010002481         3894 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_57469,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002481 DASVOR010000000
VERSION     DASVOR010002481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57469"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            588..2315
                     /locus_tag="VF516_45565"
     CDS             588..2315
                     /locus_tag="VF516_45565"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEX8115105.1"
                     /translation="MASRNPIAIPVSDRAAAPVVLAQATQPVPQTRTAAGGRPNILFI
                     MGDDIGWMQPGIYHRGLMVGETPNIYRIGRECAMFIDYHAMQSCTSGRNAFFTGMYPL
                     RTGMIPPQLPGSPTYLKPGTPALAKFLLDLGYTTGEFGKNHLGDHTDALPTAHGFQEF
                     WGYLYHLDAMQQVSFPDINRTVAEQTVAPPCRNTPIPGVPELPGAVDPRTTTCLTPPR
                     PVLHCRSASGTAEQQSCRDEGPLSLERSKTFDEEISAHVIDFLGRNDPARTGKPFFVW
                     YNPARMHVTTMLPPKYEAMLGERGGKDWGIQEAGMKQLDDNIGLVLKKLEDIGQLDNT
                     LIVFTTDNGAETMTFPDGGVTPFKGQKGEAWEGGYRAPCVVRWPGHIRPGTVLTQMFA
                     ALDWMPTLVELAGGPKGDGLKMEIQAGRYPGILKTTLDGVNQTAYLTGQSPRSAREVF
                     YYFSGATPSAVRYRNWKMYYTMSQPGAAGWILPLVPFHFTLVQNIKRDPFEQAVGVEQ
                     KSAMGLGGALGAPSTAFLYDWNMLPIGQQLWLAWFHTLQEFPPLQKPASYNLTQVMEQ
                     VKERSEPSD"
     gene            2325..>3894
                     /locus_tag="VF516_45570"
     CDS             2325..>3894
                     /locus_tag="VF516_45570"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEX8115106.1"
                     /translation="MPRQRLAWWKAVELCAAFGVFGGAMIAGRLRSTPAPPTARPSSP
                     PVVVAGLVGFVGEAVCASCHPRQAAAWTGSHHQRAMQPASEATVLGDFAGARFPHAGV
                     SSTLFRRAGAYVVRTDGPDGALHDYDVRYTFGVAPLQQYLVGFPGGRLQSPEIAWDSR
                     PAADGGQRWFHLYPGERIGPTDPLHWTGVAENWNHMCADCHSTGVRKSWSAQTGAYST
                     RFAEVSVSCEACHGPGAQHVAWASDPPDRRRAIAHEGLSIALDERAGVFWPRDPSTHK
                     PARSRPRSTEREIEMCARCHARRGLVHEDVVHGQPAGDDYRVALLDDDLYYPDGQIKG
                     EVYEYGSFVQSRMFAAGVTCSDCHDPHRGGLRAPGNGLCLSCHEPAYATPKHHFHRAG
                     SAGSQCVDCHMPPTMYMVIDRRRDHSLRVPRPDRSGKLGVPNPCNGCHANRSAGWAAR
                     TVEAWYGHTPSGLQQFAEALAAGTQGAPDAQRLLAALVADRSQPTIARASALARLERW
                     HTPATLAVVQGALDD"
BASE COUNT          618 a         1375 c         1347 g          554 t
ORIGIN      
        1 aggcgaacgc gcgcgcccgc gccgctcagc gccgcgaccg ccggatcgtc ggccggatag
       61 atcgtgtcga gccggaggcc gaggcgcgcg aacagcgcga tggctcgatc gagatcggag
      121 cagtacagga tgtcgtcgct cgtggctgtc accgggccga ggttcgcatc ttttgccgtt
      181 cggtcgccac tgcaggtgcc gggtcgccgc tcgtgcggcc tgccggaccg cccacgagcg
      241 gtgtgccgca cgccgggacc gcagagcacg ggtgcgacgg ctcccgagca ccgtgccggc
      301 ttgtctcgaa tctcacagac gaccagcgcg ctcgcgtgac gcggaagcac gggcgagatc
      361 gtgattcctg tgcggagttc aatactcttg ccctccggac gacacgggcc aaggtcgaga
      421 cgccatctgc ttggagagga cgccacatga ccaggcccca taccgcgatg tctgcgatcg
      481 cgagtccgat cgtcgccgca gccttcgcct tcacggcttg ctccgaacac ccacccgaca
      541 cgcagactga taacacgcag gacaatcaca cctcgacgag tggagatatg gcaagccgga
      601 atccgatcgc gattccggtg tcggatcgcg cggccgcgcc ggtggtcctc gcccaggcca
      661 cgcagccggt cccccagacc cggaccgccg ccggcggcag acccaacatc ctgttcatca
      721 tgggggacga catcggctgg atgcagccgg gcatctatca ccgcggtctg atggtcggcg
      781 agacgccgaa catctaccgc atcgggcgcg agtgcgcgat gttcatcgac taccacgcga
      841 tgcagagctg cacgtcgggt cgcaacgcat tcttcaccgg gatgtacccg ctgcgcacgg
      901 gcatgatccc gccccagctg ccggggagtc cgacgtacct gaagccgggc acgcccgcgc
      961 tcgccaagtt cctgctcgac ctcggttaca ccaccggcga gttcggcaag aaccacctcg
     1021 gcgatcacac cgacgcattg ccgaccgcgc acggctttca ggagttctgg ggctatctct
     1081 atcacctcga cgccatgcag caggtcagct tccccgacat caaccggacg gtcgcggagc
     1141 agacggtggc gccgccgtgc cggaacacac cgatcccggg cgtcccggag ctgcccggcg
     1201 cggtcgaccc gaggacgacc acgtgcctga cgccgccgcg cccggtgctg cactgcaggt
     1261 cggcgagcgg gaccgcggag cagcagagct gccgggacga gggcccgctc tcgctcgagc
     1321 gatcgaagac cttcgacgag gagatctcgg cccacgtcat cgacttcctc ggccgcaacg
     1381 acccggcgag gaccggcaag ccgttcttcg tctggtacaa cccggcccgc atgcacgtga
     1441 ccaccatgct gcctcccaag tacgaggcga tgctggggga gcgcggcggc aaggactggg
     1501 gcatccagga ggccgggatg aagcagctgg atgacaacat cgggctggtg ctgaagaagc
     1561 tggaggacat cggccagctc gacaacaccc tcatcgtgtt cacgaccgac aacggcgccg
     1621 agaccatgac ctttcccgat ggcggcgtga cgccgttcaa gggccagaag ggcgaggcct
     1681 gggaaggcgg ctatcgcgcg ccgtgcgtcg tccgctggcc gggtcatatc cggcccggca
     1741 cggtcctgac gcagatgttc gccgcgctcg actggatgcc gaccctggtc gagctcgccg
     1801 gaggccccaa gggcgacggg ctcaagatgg agatccaggc cgggcgctat ccggggatcc
     1861 tcaagaccac gctcgacggc gtgaaccaga ctgcctatct gacgggccag tcgccacggt
     1921 cggcgcgcga ggtgttctat tacttctccg gggcgacgcc ctccgcggtc cgttacagga
     1981 actggaagat gtactacacg atgtcgcagc ccggggcggc gggctggatc ctgccgctcg
     2041 tcccgttcca cttcacgctg gtccagaaca tcaagcgcga tccgttcgag caagcggtgg
     2101 gagtcgagca gaagtccgcg atgggcctcg gcggtgcgct gggcgccccg agcacggcgt
     2161 tcctctacga ctggaacatg ctcccgatcg gccagcagct gtggctcgcg tggttccaca
     2221 cgctgcagga gttcccgccg ctgcagaagc ctgcgagcta caacctcacg caggtgatgg
     2281 agcaggtcaa ggaacgctcc gagcccagcg actaggccca ccagatgcca cggcagcgtc
     2341 tcgcgtggtg gaaggcggtc gagctgtgcg cggcgttcgg cgtgttcggc ggggcgatga
     2401 tcgcaggccg gctgcgctcg accccggcgc cgccgaccgc gcgcccgtcg tccccgccgg
     2461 tggtggtcgc gggcctcgtc ggcttcgtcg gggaagcggt ctgcgcgtcg tgccacccgc
     2521 gccaggccgc cgcctggacc ggctcgcatc accagcgcgc gatgcagccc gcgagcgagg
     2581 cgacggtgct cggcgacttc gccggcgcgc gcttccccca cgccggcgtg tcgtcgacgc
     2641 tgttccggcg cgccggcgcg tacgtggtgc gcaccgacgg accggacggc gcactccacg
     2701 actacgacgt ccggtacacg ttcggcgtcg cgcccttgca gcagtacctg gtcgggtttc
     2761 cgggagggcg gctccagtcg ccggagatcg cgtgggacag ccggccggca gccgacggcg
     2821 gccagcgctg gtttcacctc taccccggcg agcgcatcgg ccccaccgat ccgttgcact
     2881 ggaccggcgt cgccgagaac tggaaccata tgtgcgcgga ttgccactcc acgggggtcc
     2941 gcaagtcgtg gtcggcgcag acgggcgcgt actcgacgcg gtttgcagag gtgtcggtgt
     3001 cctgcgaggc gtgccacggg ccgggcgcgc agcacgtcgc ctgggcgagc gatccgccgg
     3061 atcgccgccg ggcgattgcg cacgagggac tgtcgatcgc gctcgacgag cgcgccggcg
     3121 tgttctggcc gcgcgatccg tcgacccaca agccggcgcg cagccggccg cgcagcaccg
     3181 agcgcgagat cgagatgtgc gcgcgctgtc acgcgcggcg cgggctggtc cacgaggacg
     3241 tcgtccacgg ccagccggcg ggcgacgatt accgcgtcgc gctgctcgac gacgatctgt
     3301 attacccgga tggccagatc aaaggggaag tttacgagta cggctcgttc gtccagagcc
     3361 ggatgttcgc cgcgggcgtg acctgcagcg attgccacga cccgcaccgc gggggattgc
     3421 gcgcgccggg gaacggcctg tgcctttcgt gccacgagcc cgcctacgcg acgccgaagc
     3481 accatttcca tcgcgcggga tcagcgggct cgcagtgcgt cgattgccac atgccgccga
     3541 ccatgtacat ggtcatcgac cggcgccgcg atcacagcct gcgcgtgccg aggcccgacc
     3601 ggtcggggaa gctcggcgtg ccaaacccgt gcaacggctg ccacgcgaac cgatcggcgg
     3661 gctgggccgc gcgcacggtc gaggcatggt acgggcacac gccgtcgggc ctccagcagt
     3721 tcgccgaggc gctcgcggcg ggaacgcagg gcgcgccgga cgcgcagcgc ctgctcgcgg
     3781 cgctggtggc cgaccgcagc cagcccacga tcgcgcgcgc cagcgcactc gcgcgcctcg
     3841 agcgctggca tacccccgcg acgctcgccg tggtccaggg cgccctggac gacc
//
LOCUS       DASVOR010002482         3794 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_116248,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002482 DASVOR010000000
VERSION     DASVOR010002482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116248"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..550)
                     /gene="moaC"
                     /locus_tag="VF516_45575"
                     /pseudo
     CDS             complement(50..550)
                     /gene="moaC"
                     /locus_tag="VF516_45575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
     gene            complement(688..2529)
                     /gene="glmS"
                     /locus_tag="VF516_45580"
     CDS             complement(688..2529)
                     /gene="glmS"
                     /locus_tag="VF516_45580"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEX8115107.1"
                     /translation="MCGIIGYIGNRQATPILIGGLRKLEYRGYDSAGISVIDPTGGTG
                     DGRARVVRCRGKLGALEDKLVKEPAPGTIGMGHTRWATHGRPSDENSHPHRAGSITVI
                     HNGIIENHLALRAELVSSGAHFSSETDTEIFAHLVDRAMQAGAPDLTTAVRRSLAKVR
                     GSFAFVVMSDKEPGTLVAAKNSSPLVVGLGDGENYVASDVTAILSETRRVIFVDEGEM
                     VTVTRQGVTITDFEGKPRVREPKTITWSAVQAEKGGFKHYMLKEIHEQPRAVADTLVG
                     RVDLEHDDVLLDGIELDVKSIKRIVFIACGTSYHASLVGEFLVESLARIPVEVDLASE
                     FRYRDPILGPGDLVVAVSQSGETLDTMEAIREAKKKGARILAISNVVESSIPRLADHS
                     FYTHAGPEIGVASTKAFTTQLVAMTLFAIYLGRRTGTLSPERARELLAELVQLPTKMR
                     DVVESGAQLQVLARRYGHGGGFLFLGRGAQYPIALEGALKLKEISYIHAEGYAAGEMK
                     HGPIALIDEHLPVVVLAPRGPHYEKVVSNLAEVRARQGKVLAVATKGDAEIGGHADDV
                     ILVPDSALELQPILTVLPLQLLAYYVADFKGTDIDQPRNLAKSVTVE"
     gene            complement(2536..2706)
                     /locus_tag="VF516_45585"
     CDS             complement(2536..2706)
                     /locus_tag="VF516_45585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115108.1"
                     /translation="MMWTAGACTHASANLPVDAPRLMQYQAPDIDEITGIDSDDTDQA
                     GAHKGSAQAPHK"
     gene            3518..>3794
                     /locus_tag="VF516_45590"
     CDS             3518..>3794
                     /locus_tag="VF516_45590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115109.1"
                     /translation="MRLGAWRIERILGRDLSGTYHAAVRDDGARATLFRPSGELLVAR
                     GEPLTRLLELHRGAAQRGLIWFRAIDHDGDEPFLLADPVDDALVSLHR"
BASE COUNT          509 a         1379 c         1314 g          592 t
ORIGIN      
        1 cgtcatcccg ggcctcgcat ccggtccggc aaccggctca tcccggcggt cacggcggcc
       61 gggtcttgcc cgcgacgtga ttgccgggca gcgcccgctt ccaggtcccg gacttgccgc
      121 cgcgcttctc gtcgagccgg atctccgaga tgaccatgcc gcgatcgatc gccttgcaca
      181 tgtcgtagac ggtcaacgcc gccgcggaca ccgccgcgag cgcctccatc tcgaccccgc
      241 tgcgatcgaa cgcgcgcacc gtcgccatga tgtagaccgc gttgttcgcg acccggaggt
      301 cgacgtcgac gccggtgagc cgcagcggat ggcacagagg aatgatatcc gaagtccgct
      361 tggccgcctg gatgcctgcg atccgcgcca cacccagcac gtcgcccttg ccgatcgtgc
      421 cggcgcgcac cgcgctggcg gttgcgatct ccatcacgac catgccgctg gcgatcgcca
      481 cccgctcggt ctcgggcttg gccccgacgt cgaccatgcg ggcggcgccc gacgcgtcga
      541 agtggctgag cccgaacgtc gacgccgcct ggggcgacgc cgcttcgggg gcaggcttct
      601 ccggcggggg gtcgcgatcc ttgctgcgcg ccccgggctc gcgcgcgccg gactcgctgg
      661 cgccggatcg ggtccgctgc cgccgggtca ctcgaccgtc accgacttcg cgaggttgcg
      721 cggctgatcg atatcggtgc ccttgaagtc ggcgacgtag taggccagca gctggagcgg
      781 cagcacggtg aggatcggct ggagctccag cgccgagtcg ggcaccagga tcacgtcgtc
      841 ggcgtgcccg ccgatctcgg cgtcgccctt ggtcgcgacc gcgagcacct tgccctgccg
      901 cgcgcggacc tcggcgaggt tcgacaccac cttctcgtag tgcggcccgc gcggcgcgag
      961 caccacgacc ggcaggtgct cgtcgatcag cgcgatcggc ccgtgcttca tctcgccggc
     1021 cgcgtagccc tcggcgtgga tgtacgagat ctccttgagc ttgagcgcgc cctcgagcgc
     1081 gatcgggtac tgcgcgccgc ggccgaggaa caggaagcca ccgccgtggc cgtagcgccg
     1141 cgccagcacc tggagctggg cgccggactc gacgacgtcg cgcatcttgg tcggcagctg
     1201 gaccagctcg gcgagcagct ctcgcgcgcg ctccggcgac agcgtcccgg tccgccggcc
     1261 gagatagatc gcgaacagcg tcatggcgac gagctgcgtc gtgaacgcct tggtcgacgc
     1321 cacgccgatc tcggggccgg cgtgggtgta gaacgagtgg tcggccagcc gcgggatcga
     1381 cgactcgacg acgttggaga tcgccaggat ccgcgcgccc ttcttcttgg cctcgcggat
     1441 cgcctccatg gtgtcgaggg tctcgcccga ctggctgacc gcgaccacca ggtcgcccgg
     1501 gccgaggatc ggatcgcggt agcggaactc cgacgccagg tcgacctcga ccgggatccg
     1561 ggcgagcgac tcgaccagga actcgccgac cagcgacgcg tggtacgacg tgccgcacgc
     1621 gatgaacacg atccgcttga tcgacttgac gtcgagctcg atgccgtcga gcagcacgtc
     1681 gtcgtgctcg aggtcgaccc ggccgaccag ggtgtcggcg accgcgcgcg gctgctcgtg
     1741 gatctccttg agcatgtagt gcttgaaccc gcccttctcg gcctggaccg ccgaccacgt
     1801 gatggtcttg ggctcacgga cccgcggctt gccctcgaag tcggtgatcg tcacgccctg
     1861 gcgcgtcacc gtgaccatct cgccctcatc gacgaagatc acccggcggg tctccgacag
     1921 gatcgcggtg acgtcggacg cgacgtagtt ctcgccgtcg ccgagcccga ccaccagcgg
     1981 cgagctgttc ttggccgcga ccagcgtgcc gggctccttg tcgctcatca cgacgaacgc
     2041 gaagctgccg cgcaccttgg cgagcgagcg ccggaccgcg gtggtcagat ccggagcgcc
     2101 ggcctgcatc gcgcgatcga ccaggtgggc gaagatctcg gtgtcggtct ccgacgagaa
     2161 gtgcgctcct gacgagacca gctcggcgcg cagcgcgagg tggttctcga tgatgccgtt
     2221 gtggatcacc gtgatcgacc cggcgcggtg ggggtggctg ttctcgtcgg acggccggcc
     2281 gtgggtcgcc caccgggtgt ggcccatgcc gatcgtgccc ggcgccggct ccttgaccag
     2341 cttgtcctcc agcgcgccga gcttgccgcg gcagcgcacg acccgggcgc ggccgtcgcc
     2401 ggtgccgccg gtcggatcga tcaccgagat cccggccgag tcatagccgc gatactcgag
     2461 cttgcgcagc ccgccgatca ggatcggcgt cgcctgacgg ttaccgatgt agccgatgat
     2521 gccgcacatg ggatcctact tgtgcggggc ctgggccgag cccttgtgag cgccggcctg
     2581 gtcggtgtcg tccgagtcga tgccggtgat ctcgtcgatg tccggcgcct ggtactgcat
     2641 gagcctgggc gcgtcgaccg gcaggttggc gctcgcgtgg gtgcacgcgc cggctgtcca
     2701 catcatgcac atcaggcccc cggcccgggc ggctgtcagc agcaaagctc ggtatcgcat
     2761 ggcctgacac gctagcacgg cacggaccgg tacccggtcg gcgaaagcgc gcaaccggcg
     2821 tcggtttctc gggcgaaggc gtgtcaggcc gccacaccgt gcggcacccc gtgccggatg
     2881 tcaccagcag ccaggcgctc gccctcgcgc gtgcccaacc accaccacct acctcccgcc
     2941 gaccgagcac ccggcgcgcg cctcggtcgc ccagcgcggc atccggccgt cgctcgccgg
     3001 gctccgcgcg gtcgcggccg tcctcgaccg gatcgccgcg ccgggcgacc gctgcctccc
     3061 gtccgccgag ctggggtgtt acagatccgg atcaccgcgg cagggcggct cgatgtcgcc
     3121 cgccggcgga ccgtccgccg aggagcgccg gatgctcgtc gagtggctcg gctgcggcgc
     3181 gccgtgattc accggaatct gccgccgcgc ccttgcggtg ttggaccggt aagcgccgcc
     3241 acacgcacga cgcgttccac gaacaccaga tctttgcatc ggtcgaactc tctaccgatc
     3301 ttgcgaggcg cacactgcag ccgtgcactg gcggtgtaat cggcgcgctc gcgcgtgcag
     3361 cgtcgcaggg tgtccccgat ccgacgccgc gctttgcgcc gtcggctcac gcttgccagg
     3421 tcccgcgccg cgctacactt tgccagcatc gacgggcgcc atcgtggtag ccgcggcgcc
     3481 ggggagatcg ggagtgcaac tgatgaagga agcggtgatg cgtctgggag cctggcgtat
     3541 cgagcgcatc ctcggtcgcg atctgtccgg cacctaccac gcggcggtcc gtgacgacgg
     3601 cgcgcgcgcc acgctgttcc ggccgtcggg cgagctgctg gtggcccgag gcgagccgct
     3661 gacccggctc ctcgagctcc accgcggcgc cgcgcagcgc ggtctgatct ggttccgcgc
     3721 gatcgaccac gacggcgacg agccgttcct gctcgccgat ccggtcgacg atgcgctggt
     3781 gtccctgcac cgcg
//
LOCUS       DASVOR010002483         5068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_406379,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002483 DASVOR010000000
VERSION     DASVOR010002483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406379"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(229..2436)
                     /locus_tag="VF516_45595"
     CDS             complement(229..2436)
                     /locus_tag="VF516_45595"
                     /inference="COORDINATES: protein motif:HMM:NF027637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HEX8115110.1"
                     /translation="MRQSHAPRWVGPLWVGPLWVGTIAALAIAGCATADSPDESLIAG
                     DVLVAIPRNDLDAQAIATRDAALQARDLASSADRNDDFYLALRRNTLDQPWFLSVYLK
                     ELSPFGPNPATLGTRIVRFREQNGKLFVFNADDRLATSDVFSPDLIIDAFPIVQSSHF
                     NALPGSGAYVLIDPSAGNNHFSALADLFAAGAAPVRLSTELSFVQAFHRFDDGASYEQ
                     IVTTYAQDPIASPGGVDPNEFRLAATLGVSLRRCFESPEFKPVATPDRPHFFLSDPVN
                     VPNTGAVAQTPVHWAFARGKAPVKWLISPELAKISADPAFGGADLVAAAKRGIESWND
                     VFGFPVFSAELDTHSTSFAEDRVNYLIVDPDTSKGFAFADWRTNPVTGETRGASIYFG
                     GGFFTPLPDDAPASGAALPTPRARAAVRTLQWQGTNPAPLCVLWGLAYQAQAADAGNR
                     SLTGKQKLEAYIQHVVTHEVGHTLGLRHNFKGSLVPPTSSIMEYSVLEAAIAAPTPGP
                     YDRAAIAYLYGKSTALPTQPFATDEDTLTDPDAVRFDAPSPTPLTDYQIPQYTSFTSE
                     LLGNQFPPQVAPLIVQFFGTEVLGFVRRGTADEAATAWKAALDGARSPLASSSASAAA
                     AADALSAAVFDELYLHTTGAITAPPSDPGVVAAIAADARALLINSDGARSFATRRLVV
                     DALKKAQNQSAFLALRDARDAINTTLNGLSPSDRALTEDLLARITAAISPYFE"
     gene            2670..>5068
                     /locus_tag="VF516_45600"
     CDS             2670..>5068
                     /locus_tag="VF516_45600"
                     /inference="COORDINATES: protein motif:HMM:TIGR03960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HEX8115111.1"
                     /translation="MIYTAGRALMRHLYADFIDRVAKPMRYLGGEYQAVVKDTAEARV
                     CLAFPDVYDIGMSHLGTKIIYSLLNRDPRIACERAFAPWLDMEAELRERGLPLVSLET
                     QRPLCEFDVVGVSLQYELTFTNLLTLLDLGRIPLRAADRAEDATLVLVGGPTASHPEP
                     IAPFIDAAFIGEAEELLPPLALAWAALRRDIAAGRRARRDALAELAGRFPLYVPALYA
                     TEVDAATGMTVVGAPLDPRVPARVGRAMVADLDRFPFPSDTPVPYAEAVFERASVEIA
                     RGCTEGCRFCQAGMLYRPVRERSPASIAGSLIGGVERAGYEETGLTCLSTADFSSITP
                     LVKQLAGELRRRGVSMSVASLRAYGLNEDLLDELALTRISGLTFAPEAGTQRMRDVVN
                     KNVTEAHIEESSRRVFARGWHRLKLYFMLGLPTETDDDVRGIVETGRRMLQIGRGLVG
                     HRAEVTVSVSSHVPKPHTPLQWCAQDPEPEIRRKQAILRAAAADPRGRRDNGLRLKHH
                     HSGISFIEGVLARGDRRLAGVIEAVWRAGARFDGWDEVFELERWAAALAAHGVDVAAY
                     LGTRPVTARLPWDHIDVGLEDGFLLGEYRKALKGRASPPCGKVAGMLIHHTNLADAEP
                     DRRKLVCYDCGVACDLTKMRDDRLVALRALGATERLAPREPARIERSETRGGDPDGFA
                     GGAGGSAPRGIDDGGALVDVSERGGGSPAERAEGGDLPRAKGREGGTSDRETERAERV
                     RLAGHQGAGAPWTTYRIRFAKVGRAAFLGHLDLVRLLARSFRRADLPLAMTRGFSPKP
                     R"
BASE COUNT          688 a         1723 c         1941 g          716 t
ORIGIN      
        1 ggtgggcgca gcggcggtgg cggcgggagc cggagcggga gcagcgggat ccttggtcgc
       61 gctggacgcg gtttgatcgg tcttcgagca cgcgacagac ccaagaacga gagcggcgag
      121 cgcaagcttc ttcatggggc cgaccatata tgaacgaccg cggcgcgcaa gcgtttgaac
      181 cgcaccgccg cgcggcacgc cgggctcacc gcccccgtga cggacggctc actcgaagta
      241 ggggctgatc gccgcggtga tccgggccag caggtcctcg gtgagtgcgc ggtcggacgg
      301 gctgaggccg ttgagcgtgg tgttgatcgc gtcgcgcgcg tcgcgcaggg cgaggaacgc
      361 gctctggttc tgggccttct tgagcgcgtc gaccaccagg cggcgggtcg cgaagctgcg
      421 cgcgccgtcg ctgttgatca gcagggccct ggcatcggcg gcgatcgccg cgaccacgcc
      481 gggatcgctc ggcggcgcgg tgatcgcgcc ggtggtgtgg aggtagagct cgtcgaacac
      541 cgccgcgctc agcgcatcgg ccgccgcggc cgcgctggcc gatgaactgg ccagcggcga
      601 gcgcgcgccg tcgagcgcgg ccttccacgc ggtggcggcc tcgtcggccg tgccgcgccg
      661 cacgaagccc aggacctcgg tgccgaagaa ctggacgatc agcggcgcga cctggggcgg
      721 gaactggttg ccgagcagct ccgacgtgaa gctggtgtac tgcgggatct ggtaatcggt
      781 gagcggggtc ggcgacggcg cgtcgaaccg cacggcgtcg ggatcggtca gggtgtcctc
      841 gtcggtcgcg aacggctggg tcggcagcgc ggtcgacttg ccgtagaggt aggcgatcgc
      901 ggcgcgatcg tacggccccg gggtcggcgc ggcgatcgcg gcctcgagca cgctgtactc
      961 catgatcgac gaggtcggcg gcaccagcga gcccttgaag ttgtggcgca ggccgagcgt
     1021 gtggccgacc tcgtgggtca cgacgtgctg gatgtaggcc tcgagcttct gcttgccggt
     1081 cagcgaccgg ttgccggcgt ccgcggcctg ggcctggtag gccaggcccc acagcacgca
     1141 cagcggcgcg gggttggtgc cctgccactg cagcgtgcgg acggcggccc tggccctggg
     1201 ggtcggcagc gcggcgccgc tggccggcgc gtcatcgggc agcggggtga agaagccgcc
     1261 gccgaagtag atgctcgcgc cgcgggtctc gccggtgacc gggttggtgc gccagtcggc
     1321 gaacgcgaag cccttgctgg tgtcgggatc gacgatcagg tagttgacgc ggtcctcggc
     1381 gaacgaggtg ctgtgggtgt cgagctcggc ggagaacacc gggaagccga acacgtcgtt
     1441 ccacgactcg atgccgcgct tggccgcggc gaccaggtcg gcgccgccga acgccgggtc
     1501 ggccgagatc ttggcgagct ccggcgagat cagccacttg accggcgcct tgccgcgcgc
     1561 gaacgcccag tgcaccgggg tctgcgcgac cgcgccggtg ttggggacgt tgaccggatc
     1621 gctgaggaag aagtgcggcc ggtccggcgt cgccaccggc ttgaactcgg gcgactcgaa
     1681 gcagcggcgc aggctgacgc cgagcgtggc ggcgagccgg aactcgttgg ggtcgacgcc
     1741 gcccggcgag gcgatcggat cctgggcgta ggtggtgacg atctgctcgt acgacgcgcc
     1801 gtcgtcgaag cggtggaacg cctggacgaa cgacagctcg gtcgaaagcc ggaccggcgc
     1861 ggcgccggcg gcgaacaggt cggcgagcgc gctgaagtgg ttgttgccgg cggacgggtc
     1921 gatcaggacg taggcgccgg agccgggcag ggcgttgaag tggctgctct ggacgatcgg
     1981 gaacgcgtcg atgatgagat cggggctgaa cacgtcgctg gtcgcgagcc ggtcgtcggc
     2041 gttgaacacg aacagcttgc cgttctgctc gcggaaccgg acgatgcggg tgccgagcgt
     2101 cgccgggttg gggccgaacg ggctgagctc cttgaggtag accgacagga accacggctg
     2161 gtcgagcgtg ttgcggcgga gcgcgaggta gaagtcgtcg ttgcgatccg cgctgctcgc
     2221 caggtcgcgg gcctggagcg cggcgtcgcg cgtggcgatc gcctgggcat cgaggtcgtt
     2281 gcgcgggatc gcgaccagga cgtcgccggc gatcagcgac tcgtcgggtg agtcggcggt
     2341 ggcgcagcct gcgatcgcca gcgcggcgat cgttcctacc caaagcgggc ctacccaaag
     2401 cgggcctacc caacgcggtg cgtggctctg tctcatcggt tcctctttgc cggcccgcgg
     2461 gggatggcag ccccgggcgc ggcgaggcaa tctgtctcgg gagcagcgcg ctgggagcag
     2521 ctttacgtag gcatgtgctt acatggtgag aggtatgcgt atccgtgcta cacgactagc
     2581 caccggagac accggcaacc ccgcgcatcg gctcgccggg tcgatcgcag gcgtgcgccg
     2641 aggcacgccg ctggctgccc gggtggccgg tgatatacac cgccggccgg gccctcatgc
     2701 gacacctcta cgccgacttc atcgaccgcg tggccaagcc gatgcgctac ctcggcggcg
     2761 agtaccaggc cgtggtcaag gacaccgccg aggcgcgggt ctgcctggcg ttccccgacg
     2821 tctacgacat cgggatgtcc catctcggga ccaagatcat ctactcgctg ctcaaccgcg
     2881 atccgcggat cgcgtgcgag cgcgcgttcg cgccgtggct cgacatggag gccgagctgc
     2941 gcgagcgcgg tctgccgctg gtgtcgctcg agacccagcg gccgctgtgc gagttcgacg
     3001 tcgtcggcgt gtcgctgcag tacgagctga cgttcaccaa cctcttgacc ctgctcgacc
     3061 tcggccggat cccgctgcgc gcggccgatc gcgccgagga cgcgacgctg gtgctggtcg
     3121 gcgggccgac cgcctcgcac cccgagccga tcgcgccgtt catcgacgcc gcgttcatcg
     3181 gcgaggccga ggagctcttg ccgccgctgg cgctcgcgtg ggccgccctg cgccgcgaca
     3241 tcgccgccgg ccgccgggcc cgccgcgacg cgctcgccga gctcgccggc cggttcccgc
     3301 tctacgtgcc cgcgctgtac gcgaccgagg tcgacgccgc gaccggcatg accgtggtcg
     3361 gcgcgccgct cgatccgcgg gtgccggccc gggtcggccg cgcgatggtc gccgacctcg
     3421 accgcttccc gttcccgagc gacacgccgg tgccgtacgc cgaggcggtg ttcgagcgcg
     3481 cgtcggtcga gatcgcgcgc ggctgcaccg agggctgccg gttctgccag gccggcatgc
     3541 tgtaccggcc ggtgcgcgag cggtcgccgg cgtcgatcgc cgggtcgctg atcggcggcg
     3601 tcgagcgcgc cggctacgag gagaccgggc tgacctgcct gtcgaccgcg gacttctcca
     3661 gcatcacccc gctggtcaag cagctcgccg gcgagctcag aaggcgcggg gtgtcgatgt
     3721 cggtcgcgtc gctgcgcgcc tacggcctca acgaggacct gctcgacgag ctcgcgctga
     3781 cccggatcag cggcctgacg ttcgcgcccg aggccggcac gcagcgcatg cgcgacgtgg
     3841 tcaacaagaa cgtcaccgag gcccacatcg aggagagctc gcggcgggtg ttcgcgcgcg
     3901 gctggcaccg cctcaagctg tacttcatgc tcggcctgcc gaccgagacc gacgacgacg
     3961 tgcgcggcat cgtcgagacc ggccggcgca tgctgcagat cggccggggc cttgtcggcc
     4021 accgcgccga ggtcacggtc agcgtcagct cgcacgtgcc caagccgcac acgccgctgc
     4081 agtggtgcgc gcaggatccc gagcccgaga tccgccgcaa gcaggcgatc ctgcgcgccg
     4141 ccgccgccga tccgcgcggc cggcgcgaca acggcctgcg gctcaagcac caccacagcg
     4201 ggatctcgtt catcgagggc gtgctcgcac gcggcgatcg ccggctcgct ggcgtgatcg
     4261 aggcggtgtg gcgcgccggg gcgcggttcg acggctggga cgaggtgttc gagctcgagc
     4321 gctgggcggc cgcgctcgcc gcgcacggcg tcgacgtcgc cgcgtacctc ggcacccggc
     4381 cggtgaccgc gcgcctgccg tgggaccaca tcgacgtcgg cctcgaggac ggcttcctgc
     4441 tcggcgagta ccgcaaggcg ctcaagggcc gggcgagccc gccgtgcggc aaggtcgccg
     4501 gcatgctgat ccaccacacc aacctcgccg acgccgagcc cgatcgcaga aagctcgtgt
     4561 gctacgactg cggcgtcgcg tgcgatctga cgaagatgcg cgacgaccgg ctggtcgcgc
     4621 tgcgcgcgct gggcgccacc gagcggctcg ccccgcgcga gccggcgcgc atcgagcgaa
     4681 gcgagacgcg gggtggggac cccgacggct ttgccggcgg ggcggggggc agcgcccccc
     4741 gtgggatcga cgacggcggc gcgctggtcg acgtctccga gcgcggcggc ggcagcccgg
     4801 cggagagggc ggagggcggc gatctcccac gagcgaaggg acgtgaaggc ggtacgtcgg
     4861 accgggagac ggagcgtgca gagcgcgtcc ggctcgccgg gcaccagggc gccggggcgc
     4921 cgtggacgac ctaccgcatc cggttcgcca aggtcgggcg cgccgcgttt ctcggccatc
     4981 tcgatctggt gcggctgctc gcccgcagct ttcgtcgcgc cgatcttcca ctcgcaatga
     5041 cccgtgggtt ctctccgaag ccgcgcat
//
LOCUS       DASVOR010002484         2650 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_116262,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002484 DASVOR010000000
VERSION     DASVOR010002484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116262"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VF516_45605"
     CDS             complement(<1..561)
                     /locus_tag="VF516_45605"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8115112.1"
                     /translation="MIPIGPVPFVGRQSELTELKQHLAIVSLVSLHGPLGSGKTRLVA
                     ELAPGLGAPCTLIECHPGDRASALRSRAERALRCVPGTLAQTLAQQSRVLVIDDIHHL
                     RTDEATSLLAPLLLGPTALGRVVVVGRDPLTAAVGSAIAEIELHGLDADAAAQLWAHL
                     EETYGETQGFDAALSRTRGMPLALRRE"
     gene            complement(1141..2538)
                     /locus_tag="VF516_45610"
     CDS             complement(1141..2538)
                     /locus_tag="VF516_45610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8115113.1"
                     /translation="MKMQGVLYGVAIRPARSPWPIFRIASIAVFLVSVDATVLYAAFP
                     ALRRAFSGATAADLSWVLNAYTVVYAALLVPGGRIADLRGRKRMFLVGLGVFLLASLC
                     CGLADGVATLIAMRALQAVGAALLLPASLSIVLAAFPVERRAVAVSLWGAVAGLASAV
                     GPSLGSFLVDHWGWPWAFFLNLPLGLWAIARAAQLLDESRRPALGARLDLVGVVLLIT
                     GVGALALGVVRSEAMGWASSGVVLAIAAGAAALGAFIAWARRVPSPAIDLALFRDRTY
                     RYINVATLSFGIAFAMMFFQNFLFTTGIWHYSVAHAGLAGSPGPLFVIPASIVSGRLA
                     SRIGHKPLLMIGSLVFALGGVWFALVPGVEPDYLRTWLPGQLLNGIGVGMVMPSLAGA
                     AVAHLPPAQFGVGSAVNQAVRQIGAVLGVALTVALVGHAALARSDFRTLYACQIALAV
                     LTAVLCAPVDTRPRH"
BASE COUNT          479 a          951 c          935 g          285 t
ORIGIN      
        1 ctcgcggcgc agcgcgagcg gcatgccgcg ggtgcgcgac agcgcggcgt cgaagccctg
       61 ggtctcgccg taggtctcct cgaggtgggc ccacagctgg gccgcggcgt ccgcgtcgag
      121 gccgtgcagc tcgatctcgg cgatcgccga gccgaccgcc gcggtcagcg gatcgcggcc
      181 gaccaccacg acccggccga gcgcggtcgg gccgagcaac agcggcgcga gcaggctggt
      241 cgcctcgtcg gtgcgcaagt gatggatgtc atcgatcacc agcacgcgcg actgctgggc
      301 cagggtctgc gccagcgtgc cgggcacgca gcgcagcgcg cgctccgcgc gggatcgcag
      361 cgccgaggcc cggtcccccg ggtggcactc gatcagggtg cacggcgcgc ccaggccggg
      421 ggcgagctcg gcgaccaggc gcgtcttgcc cgatcccagc ggaccgtgca gcgacaccag
      481 cgagacgatc gcgaggtgtt gcttgagctc ggtcagctcc gactgacggc cgacgaacgg
      541 caccgggccg atggggatca tggggttcgt gatcttggag gttcgccggc gcatgactgt
      601 aagcgacgaa acagcaaggc gtgcgccatc gcgcagagaa cgaacggcga aaacgacaaa
      661 tctgcaaccc ctcgaatcaa cgtacgcgtt gtatccttgc ttgcattgtc agggaaggga
      721 ggtacagcaa cgactgcgga atcgagatct ccctgaggat cctgactggg ggtggagtcc
      781 caagtcagcc aggtccagct agtgtcagca gggtggagcg cgagcctcca ctccccctcc
      841 ctctcacgcc gacgctgatt caccatgggc tccccgggtg gccgcgccgc gtcaacgaac
      901 cgagggacgc agcgcgacgc cggtgggcga gaccccgttc cgccgtagag gagccgaaac
      961 gacctgcccg actggctgcg caacgccagc tggaacttcg gctcgttcac cgcggacaag
     1021 gcaccacgct ccggcgccaa ccaggcgatc tgcctggcct gccacaagcg acaggccctg
     1081 gtcagctacg tctacacctt caacgagctg cgcgacgcag cccacgcgcg gtgaccgcgg
     1141 tcagtggcgc ggccgggtat cgaccggcgc gcacagcacc gcggtgagca cggccagcgc
     1201 gatctggcag gcgtacagcg tccggaagtc ggaccgtgcg agcgccgcgt gcccgaccaa
     1261 cgcgacggtg agcgcgaccc cgagcaccgc gccgatctgg cgcaccgcct ggttcaccgc
     1321 gctgccgact ccgaactgcg ccggcggcag gtgggccacc gcggcgcccg ccagcgacgg
     1381 catcaccatg cccacgccga tgccgttgag cagctgcccg ggcagccagg tgcgcaggta
     1441 gtcgggctcg acgccgggga ccagcgcgaa ccagaccccg ccgagcgcga acaccaggct
     1501 gccgatcatc agcagcggct tgtggccgat gcgggacgcg agccggcccg agacgatcga
     1561 cgccgggatc acgaacagcg gccccgggct cccggcgagc ccggcgtggg cgaccgagta
     1621 gtgccagatc ccggtggtga acaggaagtt ctggaagaac atcatcgcga acgcgatgcc
     1681 gaacgacagc gtggcgacgt tgatgtagcg atacgtgcga tcgcggaaca gcgccaggtc
     1741 gatcgccggc gaggggaccc ggcgggccca ggcgatgaat gcgccgagcg ccgcggcgcc
     1801 ggccgcgatc gcgagcacca cgcccgacga cgcccagccc atcgcctcgg agcgcaccac
     1861 gccgagcgcg agcgccccga caccggtgat cagcagcacc acgccgacca ggtcgagccg
     1921 cgcgccgagc gcgggccgcc gcgactcgtc gagcagctgt gcggcgcgcg cgatcgccca
     1981 cagaccgagc gggaggttga ggaagaacgc ccacggccag ccccagtgat cgaccaggaa
     2041 cgagcccagg ctcgggccga cggcagaggc cagcccggcg accgcgcccc acaggctgac
     2101 cgcgaccgct cgccgctcga ccgggaacgc cgcgagcacg atcgacagcg acgccggcag
     2161 cagcagcgcg gcgcccaccg cctggagagc gcgcatcgcg atcagcgtcg cgacgccgtc
     2221 ggcgaggccg cagcacagcg acgcgagcag gaacacgccg agaccgacca ggaacatccg
     2281 cttgcgaccg cgcaggtctg cgatccggcc gccgggcacc agcagcgcgg cgtacaccac
     2341 ggtgtaggcg ttgagcaccc acgacagatc cgcggccgtg gcgccggaga acgcgcggcg
     2401 cagcgccggg aacgcggcat acagcacggt ggcatcgacc gagaccagga acaccgcgat
     2461 gctcgcgatc cggaagatcg gccacggcga gcgcgccggc ctgatcgcga caccatagag
     2521 aacaccctgc atcttcatga cgagctcccg gtctggacgt taaatatgat acttgacata
     2581 ttcatgggca agcccggggg cgccccgagc acatcgagga tcagccgcgg cggcgccgcc
     2641 ggccccgcgg
//
LOCUS       DASVOR010002485         1835 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_232096,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002485 DASVOR010000000
VERSION     DASVOR010002485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1835
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232096"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="VF516_45615"
     CDS             <1..981
                     /locus_tag="VF516_45615"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8115114.1"
                     /translation="EGRNAGATTGGTLTIRDLEVGRPYPVVARLEGYEPKQAVIQPQQ
                     GDNPVTLELRALAATVAVDSAPTGATVEIDGKPAGTTPLVLKTLVPSSSVALTFKKAG
                     FHDAVAKLAVPGPGKETTLIQPLAVSEELARVRLVSDPPGAQVVQNGQLVPGMVTPCE
                     LLVEAGKTTHFMLTMPHKIPAMLAPLTPPRGADDIELSGKLVDGTTMHLHANVDARFR
                     VAGAPHCQDVAAPFDCVVAPGQHIVDLIVAQAPRIARTVVVKQKDLEVKFELGYVEAS
                     GGRLVQISQGAAARRATFETGTRRVMITGGEEGPHPATVVVRPGTTTIVN"
     gene            1059..>1835
                     /locus_tag="VF516_45620"
     CDS             1059..>1835
                     /locus_tag="VF516_45620"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEX8115115.1"
                     /translation="MSEVHTQLLTDDASGALVERRCRLRVVAGPDQGKEHALEDGTTL
                     VGTHPDNDLVLTDATVSRHHLEIRVRRDGIEVRDLDTTNGTKHGGARVGQVLLVGPAR
                     LRLGRNTELDVEPVDAGVELEDWPGDRFGDALGAAPPMRRLFALLARASATEATILLT
                     GETGTGKEAIAEAVHQASPRAKGPFVVVDCGSIPHELIASELFGHARGSFTGAAADKQ
                     GLIEAASHGTLFLDEIGELALDLQPQLLRVLDRRQVRRVGE"
BASE COUNT          272 a          691 c          660 g          212 t
ORIGIN      
        1 gagggccgca acgccggcgc gacgaccggc ggcacgctga cgatccgcga cctcgaggtc
       61 ggccggccct acccggtggt cgcgcggctc gagggctacg agcccaagca ggcggtgatc
      121 cagccccagc agggcgacaa cccggtcacg ctggagctcc gcgcgctggc ggcgaccgtc
      181 gcggtcgaca gcgcgccgac cggcgcgacc gtcgagatcg acggcaagcc ggccggcacc
      241 acgccgctcg tgctcaagac cctggtgccg agctcgtcgg tcgcgctcac gttcaagaag
      301 gccgggttcc acgacgcggt cgccaagctc gcggtgcccg gcccgggcaa ggagaccacg
      361 ctgatccagc cgctcgcggt gtccgaggag ctggcccggg tccgcctggt gtccgatccg
      421 cccggcgcgc aggtcgtgca gaacggccag ctggtccccg gcatggtcac gccgtgcgag
      481 ctcctggtcg aggccggcaa gaccacgcac ttcatgctga ccatgccgca caagatcccg
      541 gcgatgctgg cgccgctcac cccgccgcgc ggcgccgacg acatcgagct gtccggcaag
      601 ctggtcgacg gcaccacgat gcacctgcac gccaacgtcg acgcccggtt ccgggtcgcc
      661 ggcgcgccgc actgccagga cgtcgccgcg ccgttcgact gcgtggtcgc gcccggccag
      721 cacatcgtcg atctgatcgt cgcgcaggcc ccgcggatcg cgcgcaccgt cgtcgtcaag
      781 cagaaggacc tcgaggtcaa gttcgagctc ggctacgtcg aggccagcgg cggcaggctg
      841 gtgcagatca gccagggcgc ggcggcgcgg cgagcgacgt tcgagaccgg cacgcggcgc
      901 gtcatgatca ccggcggcga ggaagggcct cacccggcga ccgtggtcgt gaggccgggc
      961 acgaccacga tcgtgaactg accccggcgt gaactgaccc cggcgtgaac tgaaccggcg
     1021 ggttcacgcg gcggcgcagc cgcggtacaa gacgtgacgt gagcgaagtc cacacccagc
     1081 tgctcaccga cgacgccagc ggtgcgctcg tcgagcgccg ctgccggctc cgcgtggtgg
     1141 ccgggccgga tcagggcaag gagcacgcgc tggaggacgg cacgaccctg gtcggcaccc
     1201 accccgacaa cgacctggtg ctgaccgacg ccacggtgtc gcgccaccac ctcgagatcc
     1261 gggtccggcg cgacggcatc gaggtccgcg atctcgacac caccaacggc accaagcacg
     1321 gcggcgcgcg ggtcggccag gtcctgctcg tcggcccggc ccggctgcgg ctcggccgga
     1381 acaccgagct cgacgtcgag ccggtcgacg ccggggtcga gctcgaggac tggccgggcg
     1441 atcggttcgg cgacgccctc ggcgcagcac cgccgatgcg ccggctgttc gcgctgctcg
     1501 cccgggcgtc cgccaccgag gcgacgatcc tgctcaccgg cgagaccggc accggcaagg
     1561 aggcgatcgc ggaggcggtg caccaggcca gcccgcgcgc gaaggggccg ttcgtggtcg
     1621 tcgactgcgg ctcgatcccg cacgagctga tcgcgtccga gctgttcggc cacgcccggg
     1681 gctcgttcac cggcgccgcc gccgacaagc agggcctgat cgaggccgcc agccacggca
     1741 cgctgttcct cgacgagatc ggcgagctcg cgctcgacct ccagccccag ctgctgcgcg
     1801 tgctcgaccg ccgccaggtc cgccgggtcg gcgag
//
LOCUS       DASVOR010002486        20267 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_57552,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002486 DASVOR010000000
VERSION     DASVOR010002486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 20267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20267
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57552"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VF516_45625"
     CDS             <1..234
                     /locus_tag="VF516_45625"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HEX8115116.1"
                     /translation="IREELVARDAELTSATVRRANELCRRGAAAAGADWLDLHARLAA
                     PDGELDPRYSSDGMHLSGAGYRAWAAALAPYLP"
     gene            complement(270..2111)
                     /locus_tag="VF516_45630"
     CDS             complement(270..2111)
                     /locus_tag="VF516_45630"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HEX8115117.1"
                     /translation="MDFDAIIIGSGFGGNVASSFLTKGNKRRVLMLERGTWWNSPERS
                     LPKRLEDQPVQYWPRPNHASGLIDLLSLVHTNHAPGLLEHLHDAWRGLWGFKKPQALY
                     RYNVFDDVHVVTASGVGGGSLIYSNVTLEPYHDGEKYPVMDGWSPGTQLTKADYQVAR
                     QWATTKRGPLARVVTRIPLQRAGFDGNLPAQDEFLYLAKSRVLRNASAKLKARWASLT
                     DKDLQGFTMDDWQALELALFEYEGAPVNKGGFCERQGRCLMGCLPGARQTLGKSLVAA
                     MDAGAPLTVRAGVEVTCIKRADGKSSPRRWQIAFRDLRTDQEAVATADVVVLAAGVLG
                     STEVLLRSAGGLALSDALGSKFSTNGDFLGFVIAQPQPATAIDPWYASRGPINTSHVA
                     IHNGKLHATIEDGAVPAMLAEPVRAALHVLENAKHTQSKFLEAMSALWVRRTRQELAE
                     FLPFADPWDPKRSRTEDEMMMDVFFFNCMGTDGAPGRMSLRKDRLAIEAPTLKDHPVF
                     RTLDAMMRELAGDMGGKFIPSPLWDGFGDRKLVTVHPLGGCPMGASSSDGVVNRRGQV
                     FNTRTGADTVHEGLYVIDGSIIPDALAVNPTFTIMALANRICSEAFG"
     gene            complement(2218..2661)
                     /locus_tag="VF516_45635"
     CDS             complement(2218..2661)
                     /locus_tag="VF516_45635"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX8115118.1"
                     /translation="MLWVTRHTIRINRSATAWLIRRFIDPAATFRFVPAEEVAEVQRR
                     EGARGFDAPGATYPHRDERGRCSFEALVDEHCAGDLALVELARIVHGADFADEIDYTP
                     ESAGLRAIAHGFPLVTRDDHDTLERATFLYDTLYAHLQTRRGGAA"
     gene            complement(2727..3749)
                     /locus_tag="VF516_45640"
     CDS             complement(2727..3749)
                     /locus_tag="VF516_45640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115119.1"
                     /translation="MMRTAAAGTAVLTIATVALASPQIAKPTKLALPGGEAGIGFDDL
                     MFSPSLHRVLAPAGRTGKLDLIDPATLKIESIPGFSADSDKFGGGHGEGTTSADSGGG
                     FVYASDRSRTELTLVDTKAMKIVAHQKLAGGPDYVRWVAPRSEVWVTEPGKKQIEFFA
                     LDKGALVRKGTLDVPGGPESLVIDATRGRAYTHTWDDASVAIELTSHKEVARWKNGCK
                     GSRGIALDEKRGLLFVGCDEGKATVLDLAQNGKKVGEAATGNGVDIIAYAPSLGHLYV
                     PGGDSATLTILGVGASGKLDVLGSVAVAADSHCVATDDAGHAYVCDPKSGALLVVTDP
                     FPAARK"
     gene            complement(3789..4490)
                     /locus_tag="VF516_45645"
     CDS             complement(3789..4490)
                     /locus_tag="VF516_45645"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX8115120.1"
                     /translation="MNRRQAIGSIAGTVAATAAAVRVSHAKDAKPAFAPVAPGGHKPV
                     PLPFNPAKLPGLSEKLLTSHHDNNYAGAVKALNLVELELDKVTKETHGFLVAGLRERE
                     LTYANSAYLHERYFENLGGDGKPAGDFAKALSTQFGSAARWEEAIRSTALSISGGSGW
                     AVIAHSPLTNDLRVVGTAGHAPALAAGIPILVIDMFEHSYAIDYGSAHAKYVDAFFAN
                     INWAVVEKRWQAVSK"
     gene            4545..5747
                     /locus_tag="VF516_45650"
     CDS             4545..5747
                     /locus_tag="VF516_45650"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8115121.1"
                     /translation="MSAVRARRFLYVSGFIRAMATSTAGVTMGGFLGRLDAHGTELGL
                     VVSAGLAGAAVSAVLATFAADRIGRVRFLVAVSLLGMAGTIAFALSSSPAALAAAAFL
                     GMVNGMGKDRGAALILEQAVLPGLAPASERTPAIARYTMMLDLGHALGALAAGAPAWL
                     ARTTSLTGVAPHRATFLGCAACGLVAAILYVRIGSAIEVDATPRVTLTPRSRAIVVKV
                     SSLFALDSLAGGFLTTAMLSYFFFERFHAPETFVASLFFGARILNAISHLGAAWLAKR
                     IGLVNTMVFTHIPSSILLMTVAFAPSFAVAAALFLLREGLVEMDVPTRQSYVMAVVEP
                     SERTVVSGITTLVRLTAWAIAPGFAGLLMTGDAMFLPLVIGAAMKIGYDILLWRAFRG
                     AKPPEERV"
     gene            5756..6769
                     /locus_tag="VF516_45655"
     CDS             5756..6769
                     /locus_tag="VF516_45655"
                     /inference="COORDINATES: protein motif:HMM:NF042524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HEX8115122.1"
                     /translation="MAHKRGARSGRPPREGARWVLFMPSIPSKPASARVKIWRRLQAI
                     GAVGLRGSVYVLPNREECVEVFEWVGRELGELGGQASICEGKFLDDATDDDIERRFID
                     ARNADYAEIAAAAKQLANKLEAKRVAPERIAAFTDQHAKLVRRFGEVTAIDFLHVPGR
                     EAVEGLLAAIARALPRDGTKPGEPLAVMARPSGAMWVTRTGVHVDRIASSWLIRRFID
                     PQARFKFVPAKGYAPKDGELRFDMFEAEFTHIGDRCTFEVLIERMGLRDPALIAIGEI
                     IHDLDLRDDKFGREETAGVRSTIDGICTVAHDDEQRIAAASPMLDGLYSHFSLKRRRN
                     KAT"
     gene            complement(6790..9210)
                     /locus_tag="VF516_45660"
     CDS             complement(6790..9210)
                     /locus_tag="VF516_45660"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEX8115123.1"
                     /translation="MLAAIAPVRVATAPHIDGHLDDAAWTTVPVSDSFTQSFPHDGAA
                     PSDPTRVQVAYDDDNLYIAIDCVQHTPLLARLTRRDRDVDDDRISVDLDTAHDRRSAF
                     HFQVSAAGVMVDGLRYQDTELNTDWDEIWQAEAAATQEGWSAEFAIPLRILRIHARMP
                     TWGFQVRRWVGTTGELDTWAYAPRDAGGEVSRYGELGPFEGLSPRQSIALVPFGLMRI
                     VKTDRSVPSQYGDGLSAAAGLDVTWRPRSNVAVSAAVLPDFGQVEADQLVINLTTTEI
                     EYPEKRPFFLQGMDVFQTPIQLLYTRRIGRTAAIPVLPDGVTQVQPAGAAPVVGAAKL
                     VANAGGVEIAALSALTGGVDAKVDGAVTGGAALDEVPAVLGASHHVLRARVSGEGATL
                     GVLGTAQLQRDDGARYPSVMDGALCFTGAIVTPGSSCGHDAYTGGIDAAWRSSTGTWV
                     ASGQVAGSRIENGPPLRLTDGTIVKSADGGIGSELRVAKEGGTFRGELSYSGYSRRFV
                     IDDLGYLARSNVHRLELDLEAYSAQPRGPLLETRSRIEIFGRRNLDGLMLPSGYQWNV
                     SGTGKGLWEAFVEVHWRPYYFDDREIGDGRALERAGRLGLELSLRSDPHRAVSASMSL
                     ATKSTYNGSETYLNGVIALRPRDNLELDVDPDVTIARGEPRFVDGADPAGPRFARLDA
                     TSLGVTTRATWTLQRNLTLQAYVQGLLATLRYRDAFTTDPADRVIHLDALRPAAFDPA
                     MYDVREGALNATIVGRWEYAPGSTAFLVYSHAQIPNDDGTTFDLRSLPRGPSQNVVLL
                     KLSWAWLR"
     gene            9415..10575
                     /locus_tag="VF516_45665"
     CDS             9415..10575
                     /locus_tag="VF516_45665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HEX8115124.1"
                     /translation="MSELEPVKLRQLVYYFLRLGALGFGGPIALAGYMQRDLVEQRRW
                     ISKDDYKRGLALAQLAPGPLAAQLAIYLGWVRARLLGATLVALAFIVPSFVMVLGLAA
                     AYVELGGLPWMQAVFYGVGAAVIAIIARSVQKLTKLTLAKDPLLWAVFGINVAVTALT
                     ESEIIWVFIASGVLVYLVRIRPGFATRASSFLPWTWWVTGLHGPATGATLWKLCIYFA
                     EAGAFVFGSGLAIVPFLHGGVVDHYGWLTERQFLDAVAVAMITPGPVVITVAFIGFLV
                     AGFGGASAAALGVFLPCYLFVVIPAPYYHRIADNRSIKAFVDGVSAAAAGAIGGAAIV
                     LGRRAIIDLPTIAIAAATLFVLIKLKKIPEPLVIVAAGLVGLAITSATAGAP"
     gene            complement(10646..11599)
                     /locus_tag="VF516_45670"
     CDS             complement(10646..11599)
                     /locus_tag="VF516_45670"
                     /inference="COORDINATES: protein motif:HMM:NF014796.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEX8115125.1"
                     /translation="MRYQLKQIQCRPWTLNGLSVKLIESHYENNYGGALRRLNAITEQ
                     LEALDFASTPAHVLNGLKREELVALNSTLLHELYFASMGGDGQPTKGMSEALARDFGS
                     LDRWRAEFRAMGYALGGGSGWVVLTYLPRDARLINQYASEHSQAISSGVPILALDMYE
                     HAYHLDFGANAKAYVDTFLRNIDWPALEARCEDAHKALPLRPLVQPEFRDLPSVGVEE
                     LKAMLDSGKKVQVIDVRPRHFVSRQQEIAEGIPWRDPEQLQQWIGELSKSEPVVVYCA
                     YGFHISCKTTIKLREAGFDARYMNSGHSGWKASGGPIKMHG"
     gene            12127..12441
                     /locus_tag="VF516_45675"
     CDS             12127..12441
                     /locus_tag="VF516_45675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115126.1"
                     /translation="MSGQLQAQEVQVVDLIVENIVLMQDQNNLKVPPQLQADTALFGA
                     DGFLDSMALVTLVVALEQSIEEKFGVTVTLADAAAMSQERSPYRTIQALAEYAVLRMQ
                     DN"
     gene            12461..13159
                     /locus_tag="VF516_45680"
     CDS             12461..13159
                     /locus_tag="VF516_45680"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEX8115127.1"
                     /translation="MEAPVTLITGTRVGIGRHLAHHYLALGHRVIGCSRKPVDWSHEH
                     YTHFDTDVSDEAKVKAMLSEIRRAHGRLDHLINNAGIASMNHSLLTPKSSVDAILSTN
                     VVGTFLLSREGAKLMQKQRYGRIVNFSTVAVPLKLAGEAAYVASKAAVVSLTHVMARE
                     LAELNITVNCVGPGPLETHLIRAVPKDKIARLLQHQAIPRMGTFDDVTNVVDFFLRPE
                     SSFITGQVLHLGGI"
     gene            13165..14511
                     /locus_tag="VF516_45685"
     CDS             13165..14511
                     /locus_tag="VF516_45685"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX8115128.1"
                     /translation="MFIDHLIGVFRRYPDREAIIWHDRVYHFRWLEQGVDRWTAFLEA
                     NAVARGSVVSLEADFSPDSIALLLALIERACVVVPLTASVESKKTEFREIAQVETILR
                     VDGEHAEVLHTGIEVTQDLLRELRGREHPGLVLFSSGSTGKSKAALHDLVPLLEKFTT
                     PRPALRTISFLLFDHIGGFNTLLYGLSNASCIVTVPSRLPDAVCGAIARHRVQLLPTS
                     PTFINLLLVSEAYRRHDLSSLELVTYGTEPMHESTLTRFHELYPDIRLQQTYGLSEVG
                     ILRSKSRASDSLWVRIGGEGYETRVVDGMLEIKAKSAMLGYLNAPSPFTEDGWFKTGD
                     VVQVDGEYFRILGRKSELINVGGEKVYPAEVESVLRTMPGVQGVTVSGEPHPITGQIV
                     SARVKLDSSEPLSAFRKRMFEHCKDRLPRFKIPQKVVIVSEDMHGERFKMVRPVAP"
     gene            14571..15764
                     /locus_tag="VF516_45690"
     CDS             14571..15764
                     /locus_tag="VF516_45690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115129.1"
                     /translation="METAQDAPTFAAKTSSAPAPAAARAFPAKRFLRYLVGLVIVGVT
                     AVAGANYLLDPLHFRRSALHELAATLQSGKNYAVYDANMDFRALRREAVALMTTTPQI
                     IAFGGSRFELATASMFPGRTFYNAFVHNDMYEDLVAFSGLLDDHHRLPKNLIFTVRYK
                     TFLPIDQKWRESDEFKNFWSEYRSMADRLGIDKEPLWNVLPFAYWSNLLSIDNIRRQL
                     SLSSKGGQQGPVDADSMDDMDVIHADGSMSFSKEHSASFRDLLFSRGVKNMFSSASSV
                     GEDAEQRAAKMSQLAGWPTDPGRIEGLGKLLAFLEHQGTHVTIAITPFHPAYYRRTAG
                     SPYRKSLDAMEDSVRAAAAASGSSVVGSLDPDKVGCKESSFRDFIHPDAVCLKTVFDQ
                     IAIRD"
     gene            15881..17650
                     /locus_tag="VF516_45695"
     CDS             15881..17650
                     /locus_tag="VF516_45695"
                     /inference="COORDINATES: protein motif:HMM:NF021445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyketide synthase dehydratase
                     domain-containing protein"
                     /protein_id="HEX8115130.1"
                     /translation="MTRFGRELGVAVARSSIAPCCFMARGARGAFFVNARPGTIFAFA
                     YAGPDDAFVAAARDLLAHGARDKLVVNILEQDARALALAAECEMTATPFGVLQRVPDL
                     GAWSLDGGAMRRLRQTLHHYERSGTCRTTEYAAGSDPVTDAAIVALIDDWASHKKHVG
                     PYVAHFRRGIAAGRLPERCRMFVTWRDDVVESAIVLSGMPAASGYLMDLEFYRSDLPL
                     GALEFAIWQIRQVLATESAKMFSLGSTFGTLLDACPEEDPRLGKMLRGFHASGLFNND
                     GNRQFKNKFRPVTTRLYLCRPKGAPAATITNVLAILADPEKGRPQAAPAAAPAFRTTY
                     AMSECILADHVIRGRSILPGACMIDLALAAARADRRSHALRDVVICKPGFAREDFVLE
                     VKSKGSAGFSIHAGTDLLCIGKFDDAPADAPGAAEPVDVAGGEPVDPRALYAELADLG
                     YRYGPALQVMQSAVKLATGMAFELAVADARDSRSGSMHPALLDGAIQAVFCALQRSAT
                     PIRPGGLLIPTGIRRVAMLGELLGTCWVHVADSALTRQADDVVADLRMTDAGGRSLLR
                     IDGLMLKHVPHDFLAPPSAAAPP"
     gene            17660..19141
                     /locus_tag="VF516_45700"
     CDS             17660..19141
                     /locus_tag="VF516_45700"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HEX8115131.1"
                     /translation="MLFSSLAFILAFLPLTLGGVVVARRLFGPAAARTALTIASLVFY
                     GLWGWKLLPVLVALAAFNYAWSRWLVATPKAARSRKLALGVATNLLALGVFKYTDFFI
                     ENVNTLTGQSFPLAHIALPVGISFFTFQQIGYLLAVANGTKPPQSFRDYLLFVSCFAY
                     VTAGPILTPSELFSQRDEVGNVSAQRLVVGITLFAIGLFKKGIVSEAFAPYANQLFEM
                     PRHGLAPGALDAWAGAWAYTLQLYFDFSGYSEMALGLGVMLGLRFPINFNSPLRARSA
                     IEFWQRWHITFTRFLTNYLFLPMSAKQRRKAVARKSGRFVTFLRAYGLPVMVTFVLAG
                     LWHGASWTFVLFGAWWGIALAVNHGWRQARMPAPPVALAWLITAATIVIGMVLFRAPD
                     LDTAGAILRSLVRFDLPSQLAVARIAALAIPVTALCLLAPNTPQIMDGWEITGDPIPK
                     FTGPRLLQWRWGLHGQGLAFTAFIVVALVMAGEQSSSFLYYQF"
     gene            19159..>20267
                     /locus_tag="VF516_45705"
     CDS             19159..>20267
                     /locus_tag="VF516_45705"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEX8115132.1"
                     /translation="MSSEPGPEVGDEFPVTKTCTYLNHAAIAPWPRRTQEAVARFALE
                     SAREGGRRVRQWADTERQLRGQLAQFINAPSADDIALQKNTSEALSVIAFGFPWQVGD
                     NVVIAAEEFPSNRIVWEALSDRGVGVRAVDLGAEADPEAALERACDSRTRLLSISSVQ
                     YASGLRLDLVRLGDICRSRDVAFCVDAIQGLGAIAHDVQAMQADFLVADAHKWLLGPE
                     GIAVFYCSARWRDRLRLNEYGWHMVEDPDNFDKRTWRVAASARRFECGSPNRVGIHGL
                     AASLSLLTEIGIAEIERRIAERAECIVEAVRRRAALELISSPDPRRRAGIVTFRHRRM
                     DSLRLWHHLTARNVVCAVRAGGVRFSPHFYVPLAQL"
BASE COUNT         3127 a         6939 c         6797 g         3404 t
ORIGIN      
        1 atccgcgaag agctggtcgc gcgcgacgcc gagctgacca gcgcgacggt ccggcgcgcc
       61 aacgagctgt gccggcgcgg cgccgcggcc gcgggagccg actggctcga cctccacgcc
      121 cggctggcgg ctccggatgg cgagctcgat ccgcggtact cgagcgacgg catgcacctg
      181 tcgggcgccg gatatcgcgc gtgggccgcg gcgctcgcgc cctacctgcc gtgatcgcgg
      241 gcgccgcgca gcttcctgcg cgcaagatcc tatccgaacg cctcgctgca aatgcgattg
      301 gcgagcgcca tgatggtgaa ggtcgggttg acggcgagcg catccgggat gatcgagcca
      361 tcgatgacat acaggccctc gtgtaccgtg tcggctccgg tcctggtgtt gaacacctga
      421 cctcgacgat tgacgacacc gtcgctcgat gaggctccca tcggacagcc cccgagggga
      481 tgtacggtca ccagcttgcg atcgccgaac ccatcccaca agggcgatgg gatgaacttg
      541 ccacccatgt cgccggcgag ctcgcgcatc atcgcatcca gggtcctgaa cacggggtgg
      601 tccttcaacg tcggggcctc gatcgcaagc cggtccttgc gcagcgacat ccggcccggg
      661 gcaccatcgg tacccatgca gttgaagaag aagacgtcca tcatcatctc gtcttcggtt
      721 cgcgatcgct tcggatccca gggatccgcg aacggcaaga actccgcgag ctcctgccgc
      781 gtgcgcctga cccagagcgc gctcatcgcc tcgagaaact tcgactgcgt gtgcttcgcg
      841 ttttcgagga cgtggagcgc cgcgcggacg ggctcggcca gcatcgccgg gaccgcgcca
      901 tcctcgatcg tcgcgtgaag cttgccgttg tggatcgcaa catgcgacgt gttgatcgga
      961 ccgcgactcg cgtaccacgg gtcgatcgcc gtcgcaggtt gcggctgcgc gatcacgaag
     1021 cccaggaaat cgccgttggt cgagaacttc gagccgagcg cgtcgctgag cgccagaccg
     1081 ccggcagatc ggagcaggac ctccgtgctc cccagcacgc ccgcagcgag cacgaccacg
     1141 tcggcggtcg ccacggcttc ttgatcggta cgcagatcgc ggaacgcgat ctgccatctg
     1201 cgcggtgatg acttgccgtc tgcgcgcttg atgcacgtga cctcgacccc ggccctcacg
     1261 gtgagcggcg cgccggcatc catcgccgcg acaagcgact tgccgagcgt ctggcgggct
     1321 cccggcaggc agcccatcaa acatctcccc tgcctctcac agaagccgcc cttgttcacg
     1381 ggggcgccct cgtactcgaa cagcgcgagc tccagggctt gccagtcgtc catcgtgaag
     1441 ccctgcagat ccttgtcggt cagcgacgcc cagcgcgcct tgagcttcgc gctcgcgttc
     1501 cgcaagaccc ggctcttcgc gagatacaag aactcatcct gtgccggcag gttcccgtcg
     1561 aacccggcgc gctgcagcgg tatcctcgtg accacccggg ccaggggacc acgcttggtg
     1621 gtcgcccact gccgcgcgac ctgatagtcc gccttggtca gctgcgtgcc agggctccag
     1681 ccatccatga ccgggtactt ctcaccgtcg tggtacggct ccagcgtcac gttggaatag
     1741 atcagcgatc caccacccac accgctggcc gtgacgacat ggacgtcatc aaacacgttg
     1801 tagcggtaca gtgcctgcgg tttcttgaac ccccatagcc cgcgccaggc atcgtgaaga
     1861 tgctccagca gtcccggggc atgattggtg tgaaccagcg agagcagatc gatgagtccg
     1921 ctcgcgtggt tcggtcgcgg ccagtactgg accggctgat cttcgagccg cttcggcagg
     1981 ctgcgctcgg gagagttcca ccacgtgccc cgttcgagca tgaggacgcg gcgcttgttg
     2041 cccttcgtca ggaaggacga cgcgacgttg ccaccgaacc ccgaaccaat gatgatcgca
     2101 tcgaaatcca tcacccaccc ccattgtgac ctgacaccgc cagacgaatc gcgctacgac
     2161 gtcaagcact agcacagcgc agacagagcg gcaactgacc tggcgatagc catccgctca
     2221 tgcggcgcct ccgcgccgtg tctgcaggtg cgcgtacagc gtgtcataga ggaacgtcgc
     2281 gcgctccagc gtgtcgtggt cgtcgcgcgt gacgagcggg aagccatgcg cgatcgcgcg
     2341 cagcccggcg gactccggcg tgtagtcgat ctcgtccgcg aagtcggcgc cgtggacgat
     2401 gcgcgcgagc tcgacgagcg caagatcgcc cgcgcagtgc tcgtcgacca gcgcctcgaa
     2461 tgaacagcgg ccgcgttcgt cccgatgtgg ataggtcgcg cccggcgcat cgaagccacg
     2521 cgcgccctcg cgacgctgga cctcggcgac ctcctcggcc gggacgaagc gaaacgtggc
     2581 cgcggggtcg atgaagcgcc ggatcagcca cgcggtcgcg ctgcgattga tccggatcgt
     2641 gtgcctggtg acccatagca tggcgttccc cacctttcat ggctcgacgt tgacgtcacc
     2701 gagctgctcg cggccaccag gccgcgtcac ttcctggcgg cgggaaacgg gtcggtgacc
     2761 acgagcaagg cgccgctctt gggatcgcac acgtaggcgt ggccggcgtc gtcggtcgcg
     2821 acgcagtgcg agtcggccgc caccgcgacg ctgccgagca cgtccagctt gccgctcgcg
     2881 ccgactccca ggatggtgag cgtggcgctg tcaccgccgg gaacgtagag gtgcccgagc
     2941 gacggcgcat aggcgatgat atcgacgccg ttcccggtcg ctgcctctcc gaccttcttt
     3001 ccgttctggg cgagatcgag gacggtagcc ttgccctcgt cgcagccgac gaacagcagc
     3061 ccgcgcttct catccagcgc gatcccgcgc gaccccttgc agccgttctt ccagcgcgcg
     3121 acctccttgt gcgacgtgag ctcgatcgcg acgctggcgt cgtcccaggt gtgcgtgtac
     3181 gcgcgtccgc gcgtcgcgtc gatcaccagc gactcggggc cacccggcac gtcgagcgtg
     3241 cccttgcgca cgagcgcgcc cttgtcgagc gcgaagaact cgatctgctt cttccccggc
     3301 tcggtgaccc atacctcgga tcgtggcgcg acccagcgaa cgtaatccgg cccgcctgcc
     3361 agcttctggt gcgccacgat cttcatcgcc ttggtgtcga cgagggtgag ctcggtccgg
     3421 ctgcgatcgg aggcatacac gaagccgccg ccggagtcgg cggaggtcgt gccctcgccg
     3481 tggccgccgc cgaacttgtc gctgtccgcc gagaaccccg ggatcgactc gatcttcaag
     3541 gtcgcgggat cgatcaggtc cagcttgccg gtgcgcccgg cgggcgcgag cacgcgatgc
     3601 agcgacggcg agaacatcag gtcatcgaag ccgatcccgg cctcgccccc agggagggcg
     3661 agcttggtcg gcttcgcgat ctgcgggctc gccagcgcga cggtggcgat cgtcagcact
     3721 gcggttccag cggcggcggt tcgcatcatg ggcaaggcct ttcgtgttgg gccaccagcg
     3781 caacgcggct acttgctcac ggcctgccag cgtttctcga ccacggccca gttgatgttc
     3841 gcgaagaacg cgtcgacgta cttcgcgtgg gcggagccgt aatcgatcgc gtacgagtgc
     3901 tcgaacatgt cgatcacgag gatcggtatg ccggccgcga gcgcgggcgc gtggccagcg
     3961 gtaccgacca cgcgcaggtc gttggtcagc ggcgaatgcg cgatcacggc ccagccggat
     4021 ccaccgctga tcgacagggc ggtcgaccgg atggcctcct cccagcgcgc ggccgacccg
     4081 aactgcgtcg acagcgcctt cgcgaagtcg ccggcgggct tgccatcgcc gccgaggttc
     4141 tcgaagtagc gctcgtggag atacgccgag ttcgcgtagg tcagctcgcg ctcgcgcagg
     4201 ccagcgacca ggaagccgtg ggtctccttg gtgaccttgt cgagctcgag ctcgacgagg
     4261 ttcagcgcct tgaccgcgcc cgcgtagttg ttgtcgtggt gggaggtcag gagcttctcc
     4321 gagagcccgg ggagcttcgc ggggttgaac ggcagcggga cgggcttgtg cccgccgggc
     4381 gcgaccggcg cgaaggcagg cttggcgtcc ttggcgtgtg aaacacgcac ggccgcggcg
     4441 gtagctgcaa ccgtgccggc gatcgatccg atggcttggc gtcgattcat ggccgggatg
     4501 ctaacacacg aggaacatgc gtaacgccgt gttatgacgg tttcatgtca gcggtccgcg
     4561 cgcgtcgctt cctctatgtg tcgggattca ttcgcgccat ggccaccagc accgcgggag
     4621 tgacgatggg tgggttcctc gggaggctcg acgcgcacgg gaccgagctg ggcctggtgg
     4681 tctccgccgg gctcgcgggc gccgcggtct ccgccgtgct ggcgacgttc gcggccgacc
     4741 ggatcggccg ggtgcgcttc ctggtcgccg tcagcctcct gggcatggcg gggacgatcg
     4801 cgttcgcgct gtcgagctcg ccggccgcgc tcgctgcggc ggccttcctc gggatggtca
     4861 acgggatggg caaggatcgc ggtgccgcct tgatcctgga gcaagccgtg ttgccgggtc
     4921 tcgcaccggc gagcgagcgc accccggcga tcgcccgcta caccatgatg ctcgacctgg
     4981 gccacgcgct cggtgcgctc gccgcgggcg cgccggcgtg gcttgcgcgc acgacctcgc
     5041 tgaccggcgt cgccccgcac cgggcgacgt tcctggggtg cgcggcatgc ggcctcgtcg
     5101 cggccatcct gtacgtccgg atcggcagcg cgatcgaggt cgatgcaact ccccgggtca
     5161 cgctgacgcc gcggagccgg gcgatcgtcg tgaaggtctc atcgctgttt gcgctcgata
     5221 gcctcgccgg cggattcctc accacggcga tgctgtcgta cttcttcttc gagcggttcc
     5281 acgcaccgga aacgttcgtc gcctcgctgt tcttcggcgc ccggatcctc aacgcgatct
     5341 cgcatctcgg cgcggcgtgg ctcgcaaagc gcatcggcct ggtgaacacc atggtgttca
     5401 cccacatccc gtcgagcatc ctgctcatga ccgtcgcgtt tgcaccgagc tttgccgtgg
     5461 cggcggcgct gttcctgctc cgcgaaggcc tggtggagat ggacgtgccg acgcgccagt
     5521 cgtacgtgat ggcggtcgtc gagccgagcg agcgcaccgt ggtctcgggc atcacgacgc
     5581 tggtgcggct cacggcctgg gccatcgcgc ccggcttcgc cggcctgctc atgaccgggg
     5641 acgcgatgtt tctcccgctc gtgatcgggg cggcgatgaa gatcggctac gatatcctgc
     5701 tgtggcgcgc gtttcgcggg gccaagccac ccgaggagcg cgtatgatga cggcgatggc
     5761 gcacaagcgc ggtgcacgaa gcggacgccc acccagggaa ggcgctcgat gggtcctgtt
     5821 catgccctcg atcccgtcca agcccgcgtc ggcccgcgtc aagatctggc gccggctgca
     5881 ggcgatcggc gccgtcggtc tgcgcggctc ggtgtacgtg ctgccgaatc gcgaggagtg
     5941 cgtggaggtg ttcgagtggg tgggccgtga gctcggcgag ctgggcggcc aggcatcgat
     6001 ctgcgaggga aagtttctcg atgatgcgac cgatgatgac atcgagcgcc ggttcatcga
     6061 cgcgcggaac gcggactacg ccgagatcgc ggctgcggcc aagcagcttg cgaacaagct
     6121 ggaggccaaa cgggtcgcgc cggagcggat cgccgcattc accgaccagc atgccaagct
     6181 cgtgcggcgc ttcggtgagg tcacggccat cgatttcttg cacgtgcccg gtcgcgaggc
     6241 ggtcgaaggg ctgctcgcgg cgatcgcgcg cgcgttgccg cgggacggca cgaagccggg
     6301 tgagccgctc gcggtcatgg ccaggccgag cggcgcgatg tgggtgacgc ggaccggcgt
     6361 gcacgtcgat cggatcgcga gctcgtggct gatccggcgc ttcatcgacc cccaggcccg
     6421 gttcaagttc gtgccggcga agggctacgc gccgaaggac ggcgagctgc gcttcgacat
     6481 gttcgaggcc gagttcacac acatcggcga tcgctgcacg ttcgaggtgc tcatcgagcg
     6541 catggggctc cgcgatcccg cgctgattgc gatcggcgag atcatccacg atctcgatct
     6601 gcgcgacgac aagttcggtc gcgaggagac cgcgggcgtc cgcagcacga tcgacgggat
     6661 ctgcaccgtc gcgcacgacg atgagcagcg gatcgcggcg gcatcgccga tgctcgacgg
     6721 gctgtactcg cacttctcgc tgaagcggcg gaggaacaag gcgacctgag tcagcgcacc
     6781 gcgcgcttgc tagcgcaacc atgcccagga cagcttgagc agcacgacgt tctgcgacgg
     6841 accgcgcggc agcgagcgta ggtcgaaggt cgtgccatca tcgttcggga tctgcgcgtg
     6901 cgagtacacg aggaacgccg tcgaaccggg cgcgtactcc cagcgtccga cgatggtcgc
     6961 gttgagcgcg ccctcgcgaa cgtcgtacat cgcgggatcg aaggcggcgg gccgcagcgc
     7021 gtccaggtgg atcacgcggt ccgccgggtc ggtggtgaac gcatcgcggt agcgcagggt
     7081 cgcaagcaac ccctgcacgt aagcctggag cgtcaggttg cgctgcagcg tccaggtggc
     7141 tcgcgtggtg acgccgagcg aggtcgcatc gaggcgcgcg aaccgcggtc cggctgggtc
     7201 ggcgccatcg acgaagcgcg gctcgccgcg tgcgatggtc acgtccggat cgacatcgag
     7261 ctcgaggttg tcgcgcggcc gcaacgcgat cacgccgttc agataggtct cgctgccgtt
     7321 gtaggtgctc ttggtggcaa gcgacatgct cgcggacacc gcgcggtgtg gatcgctgcg
     7381 cagcgacagc tccagcccga gccggccagc gcgctccagc gcccggccgt cgccgatctc
     7441 gcggtcgtcg aagtagtacg gtcgccagtg cacctcgaca aacgcctccc acagcccctt
     7501 gcccgtgccc gacacgttcc actgatagcc cgacggcagc atgaggccgt ccaggttgcg
     7561 gcggccgaag atctcgatgc gggaacgggt ttcgagcaac gggccgcgag gctgcgccga
     7621 gtacgcttcg aggtcgagct ccaggcggtg gacgttcgaa cgcgccaggt agccgaggtc
     7681 gtcgatcacg aaccggcgcg agtagccgga atacgacagc tcgccgcgga acgtgccgcc
     7741 ttccttggcg acgcgcagct cactgccgat gccgccgtcg gcggacttca cgatggtgcc
     7801 gtcggtgagc cggagcggcg ggccgttctc gatccgcgag ccggcgacct ggccgctcgc
     7861 gacccacgtg ccggtgctcg atcgccacgc cgcgtcgatg ccgccggtgt acgcgtcgtg
     7921 tccgcaggat gatccggggg tgacgatggc gccggtgaag cacagcgcgc cgtccatcac
     7981 cgacggatag cgcgccccgt cgtcgcgctg gagctgcgcc gtgcccagca cgccgagcgt
     8041 cgcgccttcg ccgctgacgc gggcgcgcag cacgtgatgg ctcgcgccaa gcaccgcggg
     8101 gacctcgtcc agggcggcgc cgccggtcac ggcaccgtcg accttggcat cgacaccgcc
     8161 ggtcaacgcg gagagcgctg cgatctcgac acctccggcg ttggcgacga gcttcgctgc
     8221 gccaaccacc ggggccgccc ctgcgggctg gacctgcgtc acgccatcgg gcagcaccgg
     8281 gatggctgcg gtccggccga tccggcgcgt gtagagcagc tggatcggcg tctggaacac
     8341 gtccatcccc tggaggaaga acggccgctt ctcggggtac tcgatctcgg tggtcgtcag
     8401 gttgatcacg agctggtcgg cctcgacctg gccgaagtcg ggcagcacgg ctgccgagac
     8461 ggcgacgttc gagcgcgggc gccaggtcac gtcgagaccc gccgcggcgg agagaccatc
     8521 gccgtactgg gacggcaccg agcggtcggt cttcacgatg cgcatcaggc cgaacggcac
     8581 cagtgcgatg ctctggcgag gggacagacc ctcgaacggg ccgagctcac cgtagcggga
     8641 gacctcgccg cccgcgtcgc ggggtgcgta ggcccaggtg tcgagctcgc cggtcgtgcc
     8701 gacccagcgg cgcacctgga agccccacgt cggcatgcgc gcgtgaatgc gcaggatacg
     8761 cagcgggatg gcgaactcgg cggaccatcc ttcctgggtc gcggcggcct cggcttgcca
     8821 gatctcgtcc cagtccgtgt tgagctcagt gtcctggtag cgcagtccgt cgaccatcac
     8881 acccgccgcg ctgacctgga agtgaaatgc gctgcgccgg tcgtgcgcgg tatcgagatc
     8941 gacgctgatc cggtcgtcgt cgacgtcgcg gtcacggcgg gtcaaccgcg cgagcagggg
     9001 cgtgtgctgc acgcagtcga tcgcgatgta gaggttgtcg tcgtcgtacg cgacctggac
     9061 gcgggtcgga tcgctcggcg cggcgccgtc gtgcgggaac gattgcgtga acgagtcgct
     9121 caccgggacg gtcgtccacg ccgcatcgtc gagatgtccg tcgatgtggg gcgccgtggc
     9181 aacgcgaacc ggcgcaatgg cggcaagcaa gaccgggagc atcagcatcg ggccggcaac
     9241 acctcccgcc ccccggtgcg cccggtcagc gcggcgacct cgaagcgtct cgtcgggcat
     9301 gtgcccatgt ccggatggtg catcagcccc ctcggaaaca gcaataacga tgtgttataa
     9361 acatttcatg aaccatccca gcgctcttct gcgatcggga ggcaagctcc gcgtatgagc
     9421 gagctcgagc cggtcaagct acgccagctg gtatactact tcctccgcct cggggcgctg
     9481 gggttcggcg ggccgatcgc gctcgccggc tacatgcagc gcgatctcgt cgagcagcgc
     9541 cgctggatca gcaaggacga ctacaagcgc ggcctggcgc tcgcccagct cgcgcctggt
     9601 ccgctcgccg cgcagctcgc gatctatctc ggctgggtgc gggctcggct gctgggtgcg
     9661 acgctcgtcg cgctcgcgtt catcgtgccg tcgttcgtca tggtgctcgg gctcgctgcg
     9721 gcgtacgtgg agctcggcgg gctgccgtgg atgcaggcgg tgttctacgg ggttggcgcg
     9781 gcggtgatcg cgatcatcgc gcgtagcgtg cagaagctga ccaagctcac cctggcgaag
     9841 gatccgctgc tgtgggcggt gttcgggatc aacgtggcgg tgaccgccct gaccgagtcc
     9901 gagatcatct gggtgttcat cgcgagtggc gtgctggtct acctcgtgcg tatccgcccg
     9961 ggcttcgcga cgcgggcgag ctcgttcctg ccgtggacct ggtgggtcac cggcctgcac
    10021 gggccggcca ccggcgcgac gctctggaag ctgtgcatct acttcgccga ggccggcgcg
    10081 ttcgtgttcg gcagcggcct ggcgatcgtg ccgttcctgc acggtggcgt cgtcgaccac
    10141 tacggctggc tgaccgagcg ccagttcctc gatgccgtcg cggttgcgat gatcaccccc
    10201 ggcccggtgg tgatcacggt cgccttcatc ggctttctgg tcgcaggctt cggcggcgcc
    10261 tcggcagcgg cgctcggcgt gttcttgccg tgctacctgt tcgtcgtgat cccggcgccg
    10321 tactaccatc gcatcgccga caatcgatcg atcaaggcgt tcgtcgacgg cgtctccgcg
    10381 gccgctgccg gcgcgatcgg cggcgccgcg atcgtgctcg gccgtcgcgc gatcatcgac
    10441 ctgccgacga tcgcgatcgc agcagcgacg ctgttcgtgc tgatcaagct gaagaagatc
    10501 ccggaaccgc tggtgatcgt cgccgccggt ctggtcggcc tggcgatcac gagcgcaacg
    10561 gccggagcgc catgagctgg cggacgccgt gccgcgccgt gcacctggcg tgcgaagctc
    10621 cactcgcgaa tcagagcagc ggcggtcagc cgtgcatctt gatcgggccg ccgctcgcct
    10681 tccagcccga gtggccgctg ttcatgtacc gagcgtcgaa gcccgcctcg cgcagcttga
    10741 tcgtcgtctt gcagctgatg tggaagccgt aggcgcagta caccacgacg ggttccgact
    10801 tggaaagctc acctatccac tgctggagct gttccggatc gcgccacggg atgccctcgg
    10861 cgatctcctg ctggcgcgag acgaagtgcc gcgggcgcac gtcgatgacc tgcaccttct
    10921 tcccggagtc gagcatcgcc ttcagctcct cgacgccaac gctgggcaga tcgcggaact
    10981 cgggctgcac cagcgggcgt agcggcagcg ccttgtgcgc gtcctcgcag cgcgcttcca
    11041 gcgctggcca gtcgatgttg cgcaggaacg tgtcgacgta cgcctttgca ttggcgccga
    11101 agtccagatg ataggcgtgc tcgtacatgt cgagcgcaag aatcggcacg ccgctcgata
    11161 tcgcctgcga gtgctccgag gcgtactggt tgatgagccg ggcgtcgcgc ggcaggtacg
    11221 tgaggacgac ccagcccgaa ccgccgccca gcgcgtagcc catcgcgcga aattcagcgc
    11281 gccagcgatc gagcgagccg aaatcgcgcg caagcgcttc ggacatgccc ttggtcggct
    11341 ggccgtcgcc acccatgctc gcgaagtaga gctcgtgcag cagcgtcgag ttgagcgcca
    11401 ccaactcctc gcgcttgagg ccgttgagga cgtgcgccgg cgtcgacgcg aagtcgagcg
    11461 cctcgagctg ctcggtgatg gcgttcaggc gccgcagtgc cccgccatag ttgttctcgt
    11521 agtggctctc gatcagcttg accgacagcc cgttgagggt ccacggacgg cattggatct
    11581 gcttcagctg gtagcgcatg tcgcacctct gacggtgttc gttgagaacc ttgctggcgt
    11641 gcgcgagcta tacttgtatc gtttgcgtta cgtatatatc acgtaagaaa cttgatgaga
    11701 agctgggccg gggctttctg cgcatgtcag gccgttcgac ctcaagaccg cggcgccgaa
    11761 ggtcagatcg ccatccccgg cacgagttta ctgaacgaca tcgcgctctt gccgtaccag
    11821 gcgaacgcgc cgctgtcggc gcacatgacc tggcaaggcg tcacacatgg aacgtacgtg
    11881 gcgatggccc ccgaccgggt cgagcgcgag caagacaacg gtcggatgag gtcgacgaga
    11941 atcgcaaaca cgacagcaag ccagggatct gcagcgcagg gtgcccgcag ttcgcgaccc
    12001 gcaggacgca ttcacggtcg tggtaggtcc tggcgggaac tgacgcactc cgagcaaacg
    12061 cccgcaatct gaagctaatt ttgaagatgc tttacatcat tacgagaaaa tactagcgtc
    12121 ccgcgcatgt ccgggcagtt acaagcgcag gaagtgcaag tcgtcgacct gatcgtcgag
    12181 aacatcgtgc tgatgcagga ccagaacaac ctcaaggttc ctccgcagct gcaggcggac
    12241 accgcgctgt tcggcgccga tgggttcctc gactcgatgg cgctcgtgac gctcgtcgtc
    12301 gcgctcgaac agagcatcga agagaaattc ggcgtcactg tcacgctcgc cgacgccgcc
    12361 gcgatgtcgc aagagcgcag cccgtaccgc acgatccagg cgctcgcgga atacgcagtt
    12421 ctccgcatgc aagacaactg agcgcgccgc gagaagaaac atggaagcgc ccgtcaccct
    12481 gattacaggg acacgtgtcg gcatcggccg gcacctggct caccactatc tcgcgcttgg
    12541 acaccgcgtc atcggctgca gtcgcaagcc ggtcgattgg tcgcacgagc actacacgca
    12601 tttcgacacc gacgtcagcg atgaagcgaa ggtcaaggcg atgttgtccg agatccgtcg
    12661 cgcgcacggg cggctcgatc acctgatcaa caacgccggc atcgcgtcga tgaaccactc
    12721 gctgctcacg ccgaagtcgt cggtcgacgc gatcctgagc acgaacgtcg tcggcacgtt
    12781 cctgctgtcg cgcgagggcg ccaagctgat gcagaagcag cggtacgggc ggatcgtgaa
    12841 cttctcgacg gtcgccgtgc cgctcaagct cgcgggcgag gccgcgtacg tcgcttcgaa
    12901 ggcggcggtc gtctcgctca cgcacgtgat ggcgcgcgag ctcgccgagc tcaacatcac
    12961 ggtcaactgc gtgggcccag gcccgctgga gacccacctc attcgcgcgg ttccgaagga
    13021 caagatcgcg cgcctcctgc agcaccaggc gattccgcgc atggggacgt tcgacgacgt
    13081 caccaacgtc gtcgacttct tcctccgccc ggagagcagc ttcatcaccg gacaggtcct
    13141 tcatctcggg ggcatctgac agtcatgttc atcgaccacc tgatcggcgt attccggagg
    13201 taccccgacc gcgaagcgat catctggcac gatcgcgtgt accacttccg gtggctcgaa
    13261 cagggcgtcg accggtggac cgccttcctc gaagccaacg cggtcgcgcg cggcagcgtg
    13321 gtgagcctcg aggcggactt ctcgcccgac tccatcgcgc tcctgctcgc gctcatcgag
    13381 cgcgcgtgtg tcgtcgtgcc gctcacggcc tcggtcgaga gcaagaagac ggagttccgc
    13441 gagatcgccc aggtcgagac gatcctgcgc gtcgacggcg agcacgccga ggtgctccac
    13501 accggcatcg aggtcacgca ggatctgctg cgcgagctgc gcgggcgcga gcaccccggg
    13561 ctcgtgctgt tctcctccgg ctccaccggc aagagcaagg ccgcgctgca cgacctcgtg
    13621 ccgctcctcg agaagttcac gaccccgcgc ccggcgctgc gcacgatctc gtttctgctc
    13681 ttcgatcaca tcggcggatt caacacgctg ctctacggcc tgtcgaacgc gagctgcatc
    13741 gtgaccgtcc ccagccggct cccggacgcg gtgtgcggcg cgatcgcgcg gcatcgcgtg
    13801 cagctgctgc cgacctcgcc cacgttcatc aatctcctcc tggtgagcga ggcgtaccgg
    13861 cgccacgatc tttcgagcct cgagctcgtg acgtacggca ccgagccgat gcacgagagc
    13921 acgctcacgc ggttccacga gctctatccg gacatccgcc tgcagcagac ctatgggctg
    13981 tccgaggtcg gcatcctgcg ctccaagtcg cgcgcgagcg actcgctctg ggtgcgcatc
    14041 ggcggtgagg gatacgagac ccgggtcgtc gacggcatgc tcgagatcaa ggcgaagtcc
    14101 gcgatgctcg gctacctgaa cgcgcccagc ccgttcaccg aggacggctg gttcaagacc
    14161 ggcgacgtcg tccaggtcga cggcgaatac ttccgcatcc tcggcaggaa gtccgagctc
    14221 atcaacgtcg gcggggagaa ggtgtacccc gcggaggtcg agagcgtgct ccgcacgatg
    14281 cccggggtgc agggcgtcac cgtcagcggc gagccccatc cgatcaccgg ccagatcgtg
    14341 tcggcgcgcg tgaagctcga cagcagcgag ccgctgtctg cctttcgcaa gcgcatgttc
    14401 gagcactgca aggacaggct gccgcgcttc aagatcccgc agaaggtcgt catcgtgtcc
    14461 gaagacatgc acggcgagcg tttcaagatg gtgaggcccg tcgccccgta gacgcccacc
    14521 gtcatcaccc gcagggactc acaacgtacc cgagcaccag gagaacgact ttggaaaccg
    14581 cccaagacgc gccgaccttc gccgccaaga cctcgagcgc tccggcgccc gccgctgctc
    14641 gcgccttccc ggcgaagcga ttcctgcgct acctcgtcgg tctcgtgatc gtcggggtca
    14701 cggccgtcgc cggcgcgaac tatctgctcg atccgctgca cttccgcagg tcggccctgc
    14761 atgagctcgc cgcgacgctg cagtcgggga agaactacgc cgtctacgac gccaacatgg
    14821 actttcgcgc gctgcgccgc gaagccgtcg cgctgatgac gacgacgccg cagatcatcg
    14881 cgttcggcgg cagccgcttc gagctcgcga ccgcgagcat gttcccgggc aggacgttct
    14941 acaacgcgtt cgttcacaac gacatgtacg aggacctcgt cgcgttctcg ggcctgctcg
    15001 acgaccacca ccggcttccg aagaatctga tcttcacggt tcgttacaag acgttcctgc
    15061 cgatcgacca gaagtggcgc gagtcggacg agttcaagaa cttctggtcc gagtatcgat
    15121 cgatggccga ccgcctcggc atcgacaagg agccgctgtg gaacgtcctg ccattcgcgt
    15181 actggtcgaa cctgctctcg atcgacaaca tccggcggca gctctcgctc agttcgaaag
    15241 gcgggcaaca gggcccggtc gacgccgact cgatggacga catggacgtg atccacgccg
    15301 acggatcgat gtcgttctcc aaggagcaca gcgcatcgtt ccgcgatctt ctgttctcgc
    15361 gcggcgtgaa gaacatgttc tcgagcgcgt cgagcgtggg tgaggacgcg gagcagcgcg
    15421 ccgcgaagat gagccagctc gccgggtggc cgaccgatcc cgggcgcatc gaagggctcg
    15481 gcaagttgct ggccttcctc gagcaccagg ggacccacgt gacgatcgcg atcacgccgt
    15541 tccatcccgc gtactaccgc cgcacggcgg gctcgccgta ccggaagagc ctcgacgcca
    15601 tggaggacag cgtgcgcgcc gcggcggctg ccagcggctc gagcgtcgtc ggcagcctgg
    15661 atcccgacaa ggtcggctgc aaggagtcga gcttccgcga cttcattcat cccgatgcgg
    15721 tctgtctcaa gaccgtgttc gaccagatcg cgatcaggga ttgagggcgg cgatggaacc
    15781 agacggtccg tatccggcgg cggacgaagc gcccccgtgt tggttcgagc cagaagcgct
    15841 gctcgcggcg ccgccgatcc gcgccggtgt cgattcgctg gtgacgcgat ttggccggga
    15901 gctcggcgtg gctgtcgccc ggtcgagcat cgccccgtgc tgcttcatgg cgcgcggcgc
    15961 gcggggagcg ttcttcgtga atgcgcgtcc ggggacgatc ttcgcgttcg cgtacgccgg
    16021 ccccgacgac gcgttcgtgg cggccgcgcg cgatctgctc gcgcacggcg cccgcgacaa
    16081 gctcgtcgtc aacatcctgg aacaggacgc gcgcgccctc gcgctcgccg ccgaatgcga
    16141 gatgaccgcg acgccgttcg gcgtgctgca gcgggttccg gatctcggag cgtggtcgct
    16201 cgacggcggc gcgatgcggc gcctccgcca gaccttgcac cactacgagc gatccgggac
    16261 ctgccggacg accgaatacg ccgccggcag cgatcccgtc accgatgctg cgatcgtcgc
    16321 gttgatcgac gactgggcgt cgcacaagaa acacgtcgga ccttacgtcg cgcacttccg
    16381 tcgcggcatc gcggccggcc gcctccccga gcgttgccgc atgttcgtca cgtggcgcga
    16441 cgacgtcgtg gagagcgcga tcgtcctcag cggcatgccg gctgccagcg gctacctgat
    16501 ggacctcgag ttctaccgct ccgacctgcc gctcggtgcc ctcgagttcg cgatctggca
    16561 gatccgccag gtgctcgcca ccgagagcgc gaagatgttc agcctcggct ctaccttcgg
    16621 cacgttgctc gacgcatgcc ccgaggagga tccgcgcctc ggcaagatgc tgcgcggctt
    16681 ccatgcgtcc gggctgttca acaacgacgg caaccgccag ttcaagaaca agttccggcc
    16741 cgtcaccacc cggctctatc tgtgccggcc gaagggcgcc ccggcggcca cgatcacgaa
    16801 cgtcctcgcg atcctcgccg accccgagaa gggtcgcccg caggccgctc ccgccgcggc
    16861 gccggcgttt cgaaccacgt acgcgatgtc ggagtgcatt ctcgcggatc acgtgatccg
    16921 cgggcgctcg atcctgcccg gtgcgtgcat gatcgatctc gcgctcgccg ccgcgcgcgc
    16981 cgaccgccgg agccatgcgc tgcgcgacgt ggtcatctgc aagccggggt tcgcgcgcga
    17041 agacttcgtg ctcgaggtga agtcgaaggg cagcgcgggc ttttccattc acgccgggac
    17101 ggacctgctc tgcatcggca agttcgacga tgcgccggcg gatgcaccgg gcgcagccga
    17161 gccggtcgac gtcgccggcg gcgagcccgt ggacccgcgc gcgctctacg cggagctcgc
    17221 ggacctcggc tatcgctacg gccccgcgct ccaggtcatg cagtccgcgg tgaagctcgc
    17281 gaccggcatg gccttcgagc tcgcggtcgc ggacgcgcgc gactcgcgca gcggatccat
    17341 gcatcctgcg ctcctggacg gcgcgatcca ggccgtgttc tgcgcgctgc agcgctcggc
    17401 gacgccgatc cggcccggcg gcctgctgat ccccacgggg atccgacgcg tcgcgatgct
    17461 cggcgagctg ctgggcacgt gctgggtgca cgtcgcggac agcgcgctca cccgccaggc
    17521 cgacgacgtg gtcgcggacc tgcgcatgac cgacgccggg ggccgctcgc tgctccgcat
    17581 cgacggcttg atgttgaagc acgtccccca cgacttcctc gcgccgccct cggccgcagc
    17641 acccccgtag aggcctccga tgctgttctc gtcgctcgcg ttcatcctcg cgttcttgcc
    17701 gctcacgctc ggaggcgtcg tcgtcgcccg ccggctgttc ggaccggcgg ccgcacgcac
    17761 cgcgctcacg atcgcatcgc tcgtgttcta cgggctgtgg ggctggaagc tcttgcccgt
    17821 gctcgtggcc ctcgccgcct tcaactacgc gtggtcgcga tggctcgtcg cgactccgaa
    17881 ggcggcgcgc agccgcaagc tcgcgctcgg cgtcgccacg aacctgctcg cgctcggggt
    17941 gttcaagtac accgacttct tcatcgagaa cgtcaacacg ctcaccgggc agtcgtttcc
    18001 gctggcgcac atcgcgctgc cggtcggcat ctcgttcttc acgttccagc agatcggtta
    18061 tctgctcgcc gtcgcgaatg ggaccaagcc gccccagagc ttcagggact acctgctgtt
    18121 cgtctcgtgc ttcgcgtacg tcaccgcggg cccgatcttg acgccgagcg agctgttctc
    18181 gcagcgcgac gaggtaggca acgtatccgc gcaacgactc gtcgtcggca tcaccttgtt
    18241 cgcgatcggc ctgttcaaga aggggatcgt ctccgaggcc ttcgcgccgt atgcgaatca
    18301 gctgttcgag atgccgcgtc acggcctcgc gccgggggcg ctggacgcat gggccggagc
    18361 ctgggcgtac acgctgcagc tctacttcga tttctccggc tactcggaga tggcgctcgg
    18421 tctcggcgtc atgctcgggc tgcggtttcc gatcaacttc aattcgccgc tgcgcgccag
    18481 gagcgccatc gagttctggc agcgctggca catcacgttc acgcgctttc tcacgaacta
    18541 cctgttcctt ccgatgtccg cgaaacagcg gcgcaaggcc gtggcccgga agagcggtcg
    18601 cttcgtcacc ttcctgcgcg cgtacggcct gccggtgatg gtgacgttcg tcctcgccgg
    18661 cctgtggcac ggcgcgagct ggacgttcgt gttgttcggc gcgtggtggg gcatcgcgct
    18721 cgccgtcaac cacggctggc gccaggcgcg catgccggcg ccgccggttg cgctcgcgtg
    18781 gctgatcacc gcggcgacca tcgtgatcgg gatggtgctg ttccgcgcgc ccgatctcga
    18841 cacggccggt gcgatcttgc gctcgctggt ccggttcgat cttccgtcgc agctcgccgt
    18901 cgcccgcatc gccgcgctgg cgatccccgt gaccgcgctt tgcctgctcg cgccgaacac
    18961 accgcagatc atggacggct gggagatcac cggcgatccg atcccgaagt tcacgggccc
    19021 gcggctgttg cagtggcggt ggggtctgca cggccagggg ctcgccttca ccgcgttcat
    19081 cgtcgtcgcg ctcgtgatgg ccggggagca gtcgagctcg ttcctctact accagttctg
    19141 agcgccgacc cgagtgccat gagctccgaa cccggacccg aggtcggcga cgagtttccc
    19201 gtcacgaaga cctgcacgta tctgaaccac gcggccatcg cgccctggcc gcgccgcacg
    19261 caggaggccg tcgcgcggtt tgcgctcgag agcgcgcgcg aaggcggccg ccgcgttcgc
    19321 cagtgggccg acaccgagcg ccagctgcgc ggccagctcg cgcagttcat caacgcgccg
    19381 tccgcggacg acatcgcgct gcagaagaac acctcggagg cgctctcggt gatcgcgttc
    19441 ggattcccgt ggcaggtggg cgacaacgtc gtcatcgcgg ccgaggagtt tccgtcgaac
    19501 cgcatcgtct gggaggcgct ctccgatcgc ggtgtcggcg tccgcgcggt cgatctcggc
    19561 gcggaggcgg atcccgaagc ggcgctcgag cgcgcgtgtg actcgcgcac gcggctgctg
    19621 tcgatcagca gcgtgcaata cgcgagcggg ctccgcctcg atctcgtgcg cctcggcgat
    19681 atctgccgga gccgcgacgt cgccttctgt gtcgacgcga ttcaaggtct cggcgcgatc
    19741 gcgcacgacg ttcaggcaat gcaagccgac ttcctcgtcg ccgacgccca caagtggctg
    19801 ctcggcccgg aagggatcgc cgtcttctac tgcagcgcgc gctggcgcga tcgcctgcgg
    19861 ttgaacgagt acggctggca catggtcgag gaccccgaca acttcgacaa gcgcacgtgg
    19921 cgggtcgccg ccagcgcgcg gcgcttcgag tgcggaagcc cgaaccgcgt gggcatccac
    19981 gggctcgcag cgagcctgtc gttgctcacc gagatcggca tcgccgagat cgagcgaagg
    20041 atcgccgagc gcgcggagtg catcgtcgaa gccgtccgcc gccgcgcggc gctcgagctc
    20101 atcagctcgc ccgacccgcg tcgccgcgcc ggtatcgtga cgtttcgcca tcgccgcatg
    20161 gactcgcttc gcctctggca tcacctcacg gcgcgcaacg tcgtgtgcgc ggtgcgcgcg
    20221 ggcggtgtcc gcttctcgcc ccacttctat gtaccgctgg cgcagct
//
LOCUS       DASVOR010002487         5280 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_465664,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002487 DASVOR010000000
VERSION     DASVOR010002487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465664"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..2182)
                     /locus_tag="VF516_45710"
     CDS             complement(131..2182)
                     /locus_tag="VF516_45710"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HEX8115133.1"
                     /translation="MRISLYLLIASALSAGCGPAPVDPHTSTPRGAAELRVDTSFLQA
                     FAITRVTVEAAGVSQDLVRDPSGVFVGTLLIPPGMQTLRARAFAGDTQVGASQPTAVT
                     VTTGSVTRVMLRILDTTDPPPIFGPIFDSLAFPTSLEVGVAGTFALSVVAPGGDPVTY
                     AWSSDCADSTFSAPDAATTSWSKPTPGSCAISVTATSNGFPISQNFRIVVFPANTDTG
                     AADVTAVFVSAPVVVLNLPGVQCGVSLDQNASCANTIASPQTTFYSAFVVSWGGSSPG
                     TLELSDDCGGREDRQQQNLDSIFGTWLPPVSGGLCRITARAVSSDGVAGRVVAAILAR
                     PGVPPTTQPTGPSPFAVAVADVSGDGRPDLIVAVVGPGTVSVMRGNGDGSFRTRLDAP
                     TGRFPRSVAVTDVSGDGKPDIIAVNESDNTVSVLLGNGDGTFQARVDYPTGNIPLSVA
                     VADVSGDGKPDIIVASLGTNTVNVLLGNGDGTFRAKVDYPTGTSAWAVAVADVNGDAK
                     PDIEVADIDGRTVSVLLGNGDGTFQAKVDYATGGRPSSVAVADVNGDGKPDLVTANIG
                     SDSNSATVSVLLGNGDGTFQAKVDYPTGSDPMSVAVADVSGDGIPDVVTANSSANTAS
                     VLLGHGNGTFQAKVDYSTGLFPVSVVVADVNGDGRPDLLVANENSQTVSVLLGTGSGR
                     F"
     gene            complement(2240..2938)
                     /locus_tag="VF516_45715"
     CDS             complement(2240..2938)
                     /locus_tag="VF516_45715"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEX8115134.1"
                     /translation="MECGGDLLRAWRAGDRAAGDALIAAHFASICRFFRTKLGGDVED
                     LIQQTFLDLLAASSPVINVRATLFTIARRRLLDHLRTRYQRDEVELVTSISAADLRTS
                     PSTSVARSEQHRLLHEALRHLPLDHRMALELAYWEDLSGPEIAEIMEISEHTVRSRLA
                     RARQALRDQIAALDPDTRWSSGRASVRAARSSGSARSLVRPSRQDRISARPGSQSRRS
                     RSSTPAAIPPGCAG"
     gene            complement(2872..3285)
                     /locus_tag="VF516_45720"
     CDS             complement(2872..3285)
                     /locus_tag="VF516_45720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115135.1"
                     /translation="MNLDPSLTAAAVAQAGPELNEALEQIAAMNAAISATIEPGIERS
                     KYHAGGASTEALEHDETVDPDWLCRGTDECFADPSLEVARDHVGLRWPSVPQLGQLLA
                     VCHAAHVAMIPWRPWSVVATCCVHGARGIARRGMR"
     gene            complement(3309..4640)
                     /locus_tag="VF516_45725"
     CDS             complement(3309..4640)
                     /locus_tag="VF516_45725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEX8115136.1"
                     /translation="MLAIHNLIPDSKHQVNACFHDALVLLPDAEMKSGVSVTVSQGRI
                     TRISSSCSENDLGMQHIDASGWLILPGMINAHCHSAMGFLRNKGHLRHELGAGVSTIE
                     AEMFPAERMLDPELVEALAYPFIIAGLRGGTTCFVDHYYYASSVARAMHRIGVRGFVG
                     ETIADLGGPFATDDRWRHTMTQIDSWDVPGVRPMVAPHAMDTVSRPLLEAMVGFARSR
                     DLGIHVHMSQTAGERARVYEREGASPVQVASDIGLLGERTIAAHLVSASPQDLRLFSS
                     SGAALALCPGSQILYERLAPLEEMVACAARLVIGTDCAACGDSPNLLQERRLAGLLLR
                     DRGVVDELTVVLSTDTNAARALGMPGQLGVICEGACADLVFVKPRASHRPFNNPGAWI
                     VYTLEESDVRNVMIDGYWVLWEKQALRVSEEEIEAHYEDALMRLASRRGSI"
     gene            complement(4874..>5280)
                     /locus_tag="VF516_45730"
     CDS             complement(4874..>5280)
                     /locus_tag="VF516_45730"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEX8115137.1"
                     /translation="IRSDGPDSAATPRLAGKKPHKLVVDYTSRPNEPAPPSLLAQAEE
                     DPEIGRARSAYTAGNQRLFVGDLDGAVLAYRHALELYPGYVGGYRGLGLAYEELGDTA
                     DALAALRAYVAAVPNARDVALIKKRIAHLLHR"
BASE COUNT          913 a         1780 c         1711 g          876 t
ORIGIN      
        1 cggggagcag cgcagcgggc ggacagcggg cggcttgcgc gcgcggggcc gctggtcgag
       61 ctcgagcgaa tgtacgctcg ggtcgagctc tccgccgacg acgatcccgg cgccggtcgc
      121 ggcgtgtgac tcagaaccgg ccgctgccgg tgccgagcag gacactcacc gtctgggagt
      181 tctcgttggc caccaggaga tcgggcctgc cgtcgccgtt gacgtccgcc accacaaccg
      241 acaccgggaa caagcccgtc gaatagtcga ccttggcctg gaacgtgccg ttgccatggc
      301 cgaggagtac gctggccgtg ttggcgctgc tgttggcggt cacgacatct ggaatgccat
      361 ccccgctgac gtccgccact gccacggaca tcggatccga acccgtcgga tagtcgacct
      421 tggcctggaa cgtcccatcg ccattgccga gcagcacgct caccgtcgcg ctgttgctgt
      481 cgctgccgat gttggccgtc acgaggtccg gcttgccgtc accgttcacg tccgccaccg
      541 cgaccgatga cggtcgcccg ccggtcgcgt agtcgacctt ggcctgaaac gtgccatcgc
      601 cgttgccgag cagcacgctc accgtcctgc cgtcgatgtc ggcgacctcg atgtccggct
      661 tcgcgtcgcc gttcacgtcc gccaccgcga ccgcccatgc gcttgtcccg gtgggataat
      721 cgaccttggc gcggaacgtg ccgtcgccgt tgccgagcag cacgttcacc gtgttggtcc
      781 ccaggctcgc gacgatgatg tccggcttgc catctccact cacgtccgcc accgcgaccg
      841 acagcggaat attgccggtg ggatagtcga cccgggcctg gaacgtaccg tcgccgttgc
      901 cgagcagcac gctcaccgtg ttgtcggact cgttgacggc gatgatgtcc ggcttgccgt
      961 cgccgctcac atcggtcact gcgaccgacc tcgggaatct cccggtggga gcgtcgagcc
     1021 gggtgcgaaa gctgccatcg ccgttgccac gcatcacact cacggtcccg ggccccacga
     1081 cggccacgat caggtctgga cggccgtcgc cgctgacatc cgccaccgcg accgcgaacg
     1141 gcgacgggcc ggtgggctgc gtcgtcggcg ggacgccggg gcgggccagg atcgcggcca
     1201 cgacgcgacc ggcgacgcca tcgctgctga ccgcccgcgc cgtgatgcgg cagagcccac
     1261 ccgacaccgg gggcagccag gtgccgaaga tggagtccag attctgctgc tggcggtcct
     1321 ctcgcccacc gcagtcatcg gagagctcga gggtaccggg cgagctccca ccccagctga
     1381 cgacgaaggc actgtagaac gtggtctggg gcgaggcgat cgtattcgcg caggaggcgt
     1441 tctggtcgag cgacacgccg cattgcacgc ccgggaggtt gagcacgacg acaggagccg
     1501 agacgaacac ggcggtcacg tcggcggcgc cggtgtccgt gttcgcaggg aacacgacga
     1561 tccggaagtt ctgggagatg gggaagccgt tggaggtcgc ggtgacggag atcgcgcagg
     1621 atcccggcgt cggcttggac cagctggtcg tcgcggcgtc gggcgcggag aaggtcgagt
     1681 ccgcacagtc cgagctccac gcgtaggtga ccggatcgcc accgggagcg acgaccgaga
     1741 gcgcgaaggt cccggccacc ccgacctcga gcgaggtggg aaaggccagt gagtcgaaga
     1801 tcgggccgaa gatcggcgga ggatcggtgg tatcgaggat ccgcagcatg acccgggtga
     1861 cactcccggt ggtcacggtc acggcggtcg gctggctcgc gccgacctgc gtgtcgcccg
     1921 cgaaggcgcg ggcccgcagg gtctgcatcc cgggcgggat cagcagcgtt ccgacgaaca
     1981 cgccgctcgg gtcccggacc agatcctggg agactcccgc ggcctcgacg gtgacgcgcg
     2041 tgatggcgaa cgcctggagg aagctggtgt cgacccggag ctcggcggca ccgcgcgggg
     2101 tagaggtgtg aggatccacg ggcgccgggc cacagccggc gctcagcgca gaggcgatca
     2161 ggagatagag ggagattcgc atgcccgtta ggtcgggcga ctggctcgat cgatccacga
     2221 tttcgacgat ccgtgctggc taccctgcgc atccgggcgg gatcgcggcg ggagtcgacg
     2281 atcgcgagcg tctcgactgg ctacctggcc tggcgctgat ccggtcctgc ctgctcggtc
     2341 ggacgaggga gcgggctgat ccagagcttc ttgcagccct gacagaagcc cgaccgctgg
     2401 accaccgggt gtccgggtcc agcgccgcga tctgatcgcg cagcgcctgg cgagcgcggg
     2461 ccagccgcga gcgcacggtg tgctccgaga tctccatgat ctccgcgatc tcgggtccac
     2521 tgagatcttc ccagtacgcc agctcgagcg ccatgcggtg atcgagcggc aggtggcgca
     2581 gcgcctcgtg caacaagcgg tgctgctcgc tccgcgccac cgaggtgctc ggcgatgtac
     2641 ggaggtcggc ggccgatatc gacgtgacga gctctacctc gtcgcgctga taccgcgtcc
     2701 gcaggtgatc gagcagccgg cgccgggcga tcgtgaacag cgtggcccgg acgttgatca
     2761 ccggtgacga cgccgcgagc aggtcgagga aggtctgctg gatcagatcc tccacgtcgc
     2821 cgccgagctt cgtccggaag aaccggcaga tcgatgcgaa gtgtgcagcg atcagcgcat
     2881 cccccgccgc gcgatccccc gcgcgccatg cacgcagcag gtcgccacca cactccatgg
     2941 ccgccacggt atcatggcga cgtgggcggc gtggcagact gcgaggagct ggccgagctg
     3001 cgggacgctc ggccagcgca gaccgacgtg atcgcgcgcc acctcgaggc tcggatccgc
     3061 gaagcactcg tcggtacccc ggcagagcca atccggatcg accgtttcat cgtgctcgag
     3121 cgcctcggtg ctggcaccac cggcgtggta tttgctgcgt tcgatccccg gctcgatcgt
     3181 cgccgagatg gcggcgttca tcgcggcgat ctgctccagc gcctcgttga gctctggacc
     3241 agcttgcgca acagctgccg ccgtcaatga cggatccagg ttcaacagcg acaggcgacc
     3301 gccagcgctc atatactgcc acgacgactc gcaagcctca tcagcgcatc ttcgtaatgc
     3361 gcttcaattt cttcctctga cactctcaag gcctgttttt cccataatac ccaataccca
     3421 tctatcatca catttcggac atcggactcc tcaagagtat atactatcca tgcccctggg
     3481 ttattgaatg ggcggtggct ggctcttggt ttcacgaaca ccagatcggc gcacgctcct
     3541 tcgcaaatca ccccgagttg acctggcata cccagtgcgc gtgcagcatt cgtatctgtg
     3601 gacagcacca ccgtcaactc atccacgact ccgcggtcac gcagaagcaa tccggccagg
     3661 cgacgttcct gcaacagatt tggagaatcc ccgcacgccg cacagtcagt cccgatcacc
     3721 aaccgcgcgg cgcaagcaac catctcttca agcggcgcaa gtctctcgta caggatctga
     3781 gaacccggac acaaggccaa cgccgcaccg gagctcgaga aaagccgtag atcttgcggg
     3841 ctcgctgaca ccaggtgagc tgcgatggtc cgctcaccaa ggagcccgat atcgctggcc
     3901 acttgcaccg gtgaggcacc ctcgcgctcg taaacgcgcg cccgttcgcc tgccgtttgc
     3961 gacatgtgta catgaattcc gaggtcccga cttctggcaa accccaccat ggcctccagc
     4021 agaggacgtg acacggtgtc cattgcatgt ggtgccacca tggggcgtac accgggtacg
     4081 tcccagctgt cgatctgcgt catcgtgtgg cgccagcgat catcggttgc aaacggcccg
     4141 ccgagatcgg cgatcgtctc accaacaaag ccgcgcacgc cgatgcgatg catggcgcgc
     4201 gcaaccgagc tcgcgtaata gtagtgatct acaaagcatg tcgttcctcc ccgcagcccg
     4261 gcgatgatga aggggtacgc caatgcctcc accagctccg ggtcgagcat gcgctccgcc
     4321 ggaaacatct cggcttcgat cgtgctcact cccgccccga gctcatgccg aaggtgaccc
     4381 ttattgcgca ggaatcccat ggccgaatga caatgtgcat tgatcattcc gggtagtatc
     4441 aaccagcccg aggcatctat gtgttgcatg cccagatcgt tctcgctgca tgaactggaa
     4501 attcttgtta ttctcccttg agacactgtg acgctcacgc cggacttcat ctccgcgtcc
     4561 gggagcagca caagagcatc atgaaaacac gcgttgacct gatgttttga atccgggatc
     4621 aagttgtgaa tagccagcat gttttttctc agagcccccc ctcatcactc cccgtacggg
     4681 cagcgatcct ggtcgctgga tatagcgtca acagcgaaac atcgatctga tcgttcagat
     4741 ccatacagtc gcggagcttg acacggagcc atcgccaggt ctaccgcccg ctgggaccca
     4801 ggtcgccgcg cgccaaggct cgctcccccg gagtgcagac cgaagcagac ccgaagcgga
     4861 cgggctcgac gcgtcagcgg tgcagcaggt gggcgatccg cttcttgatc agcgcgacgt
     4921 cgcgggcgtt cggtaccgcc gccacgtacg cccggagcgc ggcgagcgca tccgcggtgt
     4981 ccccgagctc ctcgtaggcc aggccgaggc cgcggtagcc gccgacgtag ccgggataga
     5041 gctccagggc gtggcgatac gcaaggaccg cgccgtcgag atccccgacg aacagccgct
     5101 gattgcccgc ggtgtacgcg ctgcgcgccc ggccgatctc cggatcctct tcggcctgcg
     5161 cgagcaagct cggcggcgca ggttcgttgg gccgcgaggt gtagtcgacg accagcttgt
     5221 ggggcttctt cccggcgagc cgcggcgtag cggcggagtc cggcccgtcg gaccggatcc
//
LOCUS       DASVOR010002488         2889 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_436056,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002488 DASVOR010000000
VERSION     DASVOR010002488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436056"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VF516_45735"
     CDS             <1..269
                     /locus_tag="VF516_45735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115138.1"
                     /translation="LDGVNDGDGDARALADRALAFCAATGLRPRISLLDYNPIGPGDR
                     FVRSPRMAAFRAVLSDAGLASHRRYSGGSDVAAACGQLAGTGAV"
     gene            276..803
                     /locus_tag="VF516_45740"
     CDS             276..803
                     /locus_tag="VF516_45740"
                     /inference="COORDINATES: protein motif:HMM:NF012922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEX8115139.1"
                     /translation="MHPVHDIAVPADLAAHVPAVIEIPRGSHLKYEVDKPTGLLRLDR
                     VLYSAVYYPANYGFIPRTHADDGDPLDILVLMQEPVEPLTIVRARAIGGLQMVDDKGG
                     DDKIVAVCVDDPAVAHYTKIAELPPHVMRELERFFTDYKVLEGKRSEVGDMYDTAQAL
                     ATIRRAREAYEGGAR"
     gene            complement(808..1431)
                     /locus_tag="VF516_45745"
     CDS             complement(808..1431)
                     /locus_tag="VF516_45745"
                     /inference="COORDINATES: protein motif:HMM:NF024809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEX8115140.1"
                     /translation="MTAAMLLYHDTRAPNPRRVRIFLAEKGVAYDTIEVSIAAAEHQK
                     PEFRKKNPLALLPVLELGDGKILRESMAISRYVEELFPEPNLLGADPWERAVIEQWNR
                     HAELELLLPIAQVFRNTNAFWQGRIRQSPEFGEIMREQVLGRFDWLEQELAQREYLAG
                     PRFTVADITALCAIDFGKVSSLRIKAETHPSLAAWHARVSGRPSAKA"
     gene            1494..2399
                     /locus_tag="VF516_45750"
     CDS             1494..2399
                     /locus_tag="VF516_45750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115141.1"
                     /translation="MRIARVIAALWFVGGLAACKDRSEASAAPDLQALKAQQDLMKRR
                     DALLEQRQKLESQRKVVLEQIEKIQASGGDTKELTRQRDDLTAQIDGQSAELGQMSDK
                     LSSLSTKLDSAAELAVREDRLAQREGRLAAREASVGERERGLAQREEKLAQREKETCG
                     TAPPMIIQTARADGKYSKADVQPLLARARATMSRKGILVADLPAPAQGLEGESTRAMA
                     DNDWGKAYFAAAQLVAMVDAVKIDRAFIQTKTARLSNQVKASKPDDATNQQLTGLLSD
                     VMQRYNDGNFAAANVRLNQLAAMLK"
     gene            complement(2422..>2889)
                     /locus_tag="VF516_45755"
     CDS             complement(2422..>2889)
                     /locus_tag="VF516_45755"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEX8115142.1"
                     /translation="DDGGGPSGAAMRLLDPDGYRGTLRFAIDVTGATVYVNGGKALLS
                     PRGELALPVGTHAVRVTHPEYHDFVRFIDAECGKTTNVAVYLQQYPILRHDVPGKPVN
                     RDRIEYVDPPVWRRWYVVGPAAVGLAILTGVVVGYAVHDFPDGDCRKLGGGHC"
BASE COUNT          448 a         1019 c         1039 g          383 t
ORIGIN      
        1 tgctcgacgg cgtcaacgac ggcgacggcg atgcccgtgc gctcgccgac cgcgcgctcg
       61 cgttttgcgc cgcaaccggg ctccggccgc ggatctcgct gctcgactac aacccgatcg
      121 gcccgggcga tcggttcgtc cgctcgccgc gcatggccgc gttccgcgcc gtgctgtcgg
      181 acgccggcct ggcctcgcac cggcgctact cgggcggctc ggatgtcgcc gcggcgtgcg
      241 gccagctcgc cggcaccggc gcggtataac gccgcatgca cccggtccac gacatcgccg
      301 tccccgccga cctcgccgcg cacgtgccgg cggtgatcga gatcccgcgc ggctcgcacc
      361 tcaagtacga ggtcgacaag ccgaccggcc tgctccggct cgaccgcgtg ctgtacagcg
      421 cggtgtacta cccggccaac tacggcttca tcccccggac gcacgccgac gacggcgatc
      481 cgctcgacat cctcgtgctg atgcaggagc cggtcgagcc gctgaccatc gtgcgcgccc
      541 gcgcgatcgg cgggctgcag atggtcgacg acaagggcgg cgacgacaag atcgtcgcgg
      601 tgtgcgtcga cgaccccgcg gtcgcccact acaccaagat cgccgagctg ccgccgcacg
      661 tcatgcgcga gctcgagcgg ttcttcaccg actacaaggt gctcgagggc aagcgcagcg
      721 aggtcggcga catgtacgac acggcgcagg cgctcgcgac gatccggcgg gcgcgcgagg
      781 cctacgaggg cggcgcgcgc tgacccccta cgccttcgcg ctcggccggc cgctgacccg
      841 ggcgtgccag gcggcgaggc tggggtgggt ctcggccttg atccgcaggc tggacacctt
      901 gccgaagtcg atcgcgcaca gcgcggtgat gtcggcgacg gtgaaccgcg gcccggcgag
      961 gtactcccgc tgggcgagct cctgctcgag ccagtcgaac cggccgagca cctgctcgcg
     1021 catgatctcg ccgaactcgg gggactgccg gatccggccc tgccagaacg cgttggtgtt
     1081 gcggaacacc tgggcgatcg ggagcaggag ctcgagctcg gcgtgccggt tccactgctc
     1141 gatcaccgcg cgctcccacg ggtcggcgcc cagcaggttg ggctcgggga acagctcctc
     1201 gacgtagcgc gagatcgcca tcgactcgcg caggatcttg ccgtcgccga gctccagcac
     1261 cgggagcagg gcgagcgggt tcttcttgcg aaactccggc ttctggtgct cggccgccgc
     1321 gatcgagacc tcgatcgtgt cgtatgccac acccttctcg gccaggaaga tccgcacccg
     1381 ccgcgggttg ggggctcggg tgtcgtggta gaggagcatc gccgcggtca tggccgcact
     1441 ctacgaccaa aaccgccgcc ctggcccgag gcgctgtcgt agaattgccg ggtatgcgga
     1501 tcgctcgggt catcgcagcg ctgtggttcg tcggtgggct cgcggcctgc aaggaccgga
     1561 gcgaggccag cgcggccccg gatctgcagg cgctcaaggc ccagcaagat ctgatgaagc
     1621 ggcgcgacgc gctgctcgag cagcgccaga agctggagag ccagcgcaag gtggtgctgg
     1681 agcagatcga gaagatccag gcgtcgggcg gcgataccaa ggagctgacc cggcagcgcg
     1741 atgacctgac cgcgcagatc gacggtcagt cggccgagct cggccagatg tccgacaagc
     1801 tgtcgagcct gtcgaccaag ctcgactcgg ccgccgagct cgcggtccgc gaggaccggc
     1861 tggcgcagcg cgagggcagg ctggcggcgc gcgaggcctc cgtcggcgag cgcgagcgtg
     1921 gcctggcaca gcgcgaggag aagctggcgc agcgcgagaa ggagacctgc ggcaccgcgc
     1981 cgccgatgat catccagacc gcccgcgccg acggcaagta cagcaaggcc gacgtccagc
     2041 cgctgctcgc ccgcgcccgc gcgacgatgt ccaggaaggg catcctggtc gccgacctgc
     2101 cggcgccggc gcagggcctc gagggcgagt cgacgcgggc gatggccgac aacgactggg
     2161 gcaaggcgta cttcgccgcg gcacagctgg tcgcgatggt cgacgcggtc aagatcgacc
     2221 gcgcgttcat ccagaccaag accgcgcggc tgtccaacca ggtcaaggcg agcaaacccg
     2281 acgacgcgac caaccagcag ctgaccggcc tcctgtccga cgtgatgcag cgctacaacg
     2341 acggcaactt cgccgccgcc aacgtccggc tgaaccagct ggcggcgatg ctgaagtgag
     2401 ctgagccacg cagagcagac gctagcagtg gccgccgccg agcttgcggc agtcgccgtc
     2461 cgggaagtcg tgcacggcgt agccgaccac gacgccggtg aggatcgcga ggccgaccgc
     2521 ggcggggccg accacgtacc accggcgcca caccggcggg tcgacgtact cgatccggtc
     2581 gcggttgacc ggcttgccgg gcacgtcgtg gcggaggatc gggtactgct gcaggtagac
     2641 cgcgacgttg gtggtcttgc cgcactcggc gtcgatgaac cgcacgaagt cgtggtactc
     2701 ggggtgggtg acgcgcaccg cgtgggtgcc gaccggcagc gcgagctcgc cgcgcggcga
     2761 cagcagggcc ttgccgccgt tgacgtagac cgtcgcgccg gtgacgtcga tcgcgaaccg
     2821 cagcgtgccg cggtagccgt cggggtcgag cagccgcatc gcggcgccgc tcgggccacc
     2881 gccgtcgtc
//
LOCUS       DASVOR010002489         1775 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_173718,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002489 DASVOR010000000
VERSION     DASVOR010002489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1775
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173718"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..>1775
                     /locus_tag="VF516_45760"
     CDS             169..>1775
                     /locus_tag="VF516_45760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115143.1"
                     /translation="MFLAAVSAACAAPRPFPLRAPLAVDTDLLPVSVPCRLDPAAKDD
                     HPWNCAPREYVSPFVWDQVDNLWFAPLSRALSVEASGEAVNANSLDEVADSAWFTNRP
                     RAGEVDAEADAAAPGACTPDDVLAAPDHVHDGAWLIDHGKDNGSTLGFRVKVPEKGTY
                     MLKADDEGKPERASAASVIGAAIYHALGYYTSCEQIVVLRKGQLKLSPGLMVVANSGV
                     SHPFDDKALDKVLATTTQLPGHRVRMQASKWLPGLAIGPFRYTGTRDDDPNDVIDHAD
                     RRELRGMRVLAAWLDHWDAREQNSMDVWLASDPGDKRSSPGHVVHYVLDTSDTLGGDV
                     SIAEMSRRLGYSYDLDFAEIGRSLITLGAEERPWDHARVAPGREKFNYYRADDFDPAA
                     WKPMYPNPAFVRMTERDAAWMARLIARFSHDDIRRFVALGKWQDPSDAGYLANILIER
                     QRRILVRYLGKLSPLGEVRAAGRDRICATDFARLREIAAPASFRYTIVERGGGRTLEL
                     PAEVGAGGEVCFRPRAVVTGELPDADPG"
BASE COUNT          249 a          663 c          618 g          245 t
ORIGIN      
        1 ccgcgcgccg acgctcccgc cgcctgcgcg gccgtggcac gtcggggcgg cagcgcccgc
       61 atgctgcggt gagccaagcc agggtgtctc ggagattccg ctggcgggcg ccttgcacca
      121 gcactcgcca ttcgtccatc cacttcattc ccagccctgt tccccgctgt gttcctcgcc
      181 gcggtgtccg ccgcctgcgc agcgcctcgt ccgttcccgc tgcgcgcgcc gctcgcggtc
      241 gacaccgatc tgctgccggt gtctgtgccg tgccggctcg atccagcggc caaggacgac
      301 cacccctgga actgcgcgcc gcgcgagtac gtctcgccgt tcgtgtggga ccaggtcgac
      361 aacctgtggt tcgcaccgct gtcgcgcgcg ctgtcggtcg aggcctcggg cgaggcggtc
      421 aacgccaaca gcctcgacga ggttgccgac tcggcgtggt tcaccaaccg gccgcgcgcc
      481 ggtgaggtcg acgccgaggc cgacgccgcg gcgcccgggg cgtgcacccc cgacgacgtg
      541 ctggcggccc cggaccacgt gcacgacggc gcgtggctga tcgatcacgg caaggacaac
      601 ggctcgaccc tgggcttccg cgtcaaggtc cccgagaagg gcacctacat gctcaaggcc
      661 gacgacgagg gcaagccgga gcgcgcgagc gccgcgagcg tgatcggcgc ggcgatctac
      721 cacgcgctcg gctactacac gagctgcgag cagatcgtcg tgctccgcaa gggccagctc
      781 aagctgagcc cggggctcat ggtcgtcgcg aacagcggcg tcagccaccc gttcgacgac
      841 aaggcgctcg acaaggtgct ggcgaccacg acccagctgc ccggccaccg ggtgcggatg
      901 caggcgtcga agtggctgcc cggcctggcg atcggaccgt tccgctacac cggcacgcgc
      961 gacgacgatc ccaacgacgt gatcgaccac gccgatcgcc gcgagctgcg cggcatgcgg
     1021 gtcctcgcgg cctggctcga tcactgggac gcccgcgagc agaactcgat ggacgtgtgg
     1081 ctggccagcg atccgggcga caagcggtcg tcgcccggcc acgtcgtgca ctacgtcctc
     1141 gacaccagcg acacgctggg cggcgacgtc agcatcgccg agatgtcgcg ccggctcggc
     1201 tactcctatg acctcgactt cgccgagatc ggccgctcgc tgatcacgct gggcgccgag
     1261 gagcggccgt gggaccacgc ccgcgtggcg ccgggccgcg agaagttcaa ctactaccgc
     1321 gccgacgact tcgatcccgc ggcgtggaag ccgatgtacc ccaacccggc gttcgtgcgg
     1381 atgaccgagc gcgacgccgc ctggatggcg cggctgatcg cgcggttctc gcacgacgac
     1441 atccgacggt tcgtcgcgct cggcaagtgg caggatccga gcgacgccgg ctacctcgcg
     1501 aacatcctga tcgagcgcca gcgccggatc ctggtccgct acctcggcaa gctgtcgccg
     1561 ctcggcgagg tccgcgccgc cggccgggac cggatctgcg cgaccgactt cgcccggctg
     1621 cgcgagatcg ccgcgccggc gagcttccgc tacacgatcg tcgagcgcgg cggcggccgc
     1681 acgctcgagc tgcccgccga ggtcggcgcc ggtggggagg tgtgcttccg gcctcgggcc
     1741 gtggtcaccg gcgagctgcc cgacgccgat ccggg
//
LOCUS       DASVOR010002490         1645 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_495507,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002490 DASVOR010000000
VERSION     DASVOR010002490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1645
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495507"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1161)
                     /locus_tag="VF516_45765"
     CDS             complement(<1..1161)
                     /locus_tag="VF516_45765"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8115144.1"
                     /translation="MADLDRLGLIGQTVDGRYTILEQIGEGGMGAVYRAEHQRLGRQV
                     AIKFLHQAYAKDHEALDRFFNEARAAATLGHPNIAESFDVGELSDGSPFIVLELLQGR
                     SIHAEVRRTGRLSIRRALRIARQIALGLDAAHQRGIVHRDLKSENVHLVERGDTPDHV
                     KILDFGVARILENSASITQPGHLIGSPEVMPPEQILSPHTVDARVDIYAAGVILYQML
                     SGHKPFESVPMPALFHRILEEPPPPLTFLPDDVRALVEDAMARDRDQRIQTANELRDR
                     IDACLAASPGTPLRTPAPEDIDITGPAPADVTSPSLLAAPVPTIVDRRGGEGRYRSLK
                     IRAAGAAGTFVIAGAVFAVVPLTSHSPGPAPTAPPPSPAGEVAMNPAAPPRAE"
     gene            complement(1352..>1645)
                     /locus_tag="VF516_45770"
     CDS             complement(1352..>1645)
                     /locus_tag="VF516_45770"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8115145.1"
                     /translation="AARDQAAETWRRVRSERARVSAMLRQLGFALAAESQSNFVLAAV
                     PAHASAPDLQQALEQRGVLVRYFAEPRLRASLRITIGTPEQNDRLLGALRELV"
BASE COUNT          235 a          594 c          584 g          232 t
ORIGIN      
        1 ctcggccctc ggcggagcgg ccgggttcat cgcgacctca cccgccgggg acgggggcgg
       61 cgccgtcggg gctgggcctg gggaatggct ggtcaggggc acgacggcga acacggcgcc
      121 ggcgatcacg aaggtgccgg ccgcgcccgc cgcgcggatc ttcaggctgc ggtaccgccc
      181 ctcgccgccg cgccggtcca cgatcgtcgg caccggcgcc gcgagcaagg acggcgaggt
      241 gacatcggcc ggcgcgggtc cggtgatgtc gatgtcctcc ggggccgggg tccgcagcgg
      301 tgtccccggg ctcgcggcca ggcaggcgtc gatccgatcg cgcagctcgt tcgcggtctg
      361 gatccgctgg tcgcggtcgc gcgccatcgc gtcctcgacc agcgcgcgca cgtcgtcggg
      421 caagaacgtc agcggcggcg gcggctcctc gaggatccgg tgaaacagcg cgggcatcgg
      481 caccgactcg aacggtttgt gccccgacag catctggtag aggatcacgc ccgctgcgta
      541 gatatcgacc cgcgcgtcga ccgtgtgggg cgacaggatc tgctcgggcg gcatcacctc
      601 gggcgagccg atcagatgcc ccggctgggt gatgctggcc gagttctcga ggatccgggc
      661 gaccccgaaa tcgaggatct tgacgtgatc gggagtgtcc ccccgctcga ccaggtgcac
      721 attctccgac ttgagatcgc gatggacgat cccgcgctgg tgcgccgcgt cgagacccag
      781 cgcgatctgg cgcgcgatcc gcagcgcgcg tcggatcgac agccggccgg tgcggcggac
      841 ctcggcgtgg atcgaccggc cctggaggag ctccagcacg atgaacggcg acccgtcgga
      901 tagctcgccg acgtcaaacg attccgcgat gttgggatgg cccagggtcg ccgcggcccg
      961 cgcctcgttg aagaaccggt cgagcgcctc gtgatccttg gcgtaggcct ggtgcaggaa
     1021 cttgatcgcg acctgccgcc cgagccgctg gtgctcggcg cgatagaccg cgcccatgcc
     1081 gccctcgccg atctgctcga ggatcgtgta gcggccatcc acggtttggc cgatgagccc
     1141 cagccgatcc agatctgcca ccaggctcga atgtaccgaa gcccctcaca gcgttcaacg
     1201 cacagcgcgc tccaccctcg ggaagacccg cctcgccatc cggggcaacg tgccccgtat
     1261 cccatgcatg ggatcctcgc cggctccgga gtcgctgaac aagtgcatcc gggacggact
     1321 gcccgaccac accggcctgc cgatctggcg atcagaccag ctcgcgcagc gccccgagca
     1381 ggcgatcgtt ctgctccggc gttccgatcg tgatccgcag cgaggcgcgc agccgcggct
     1441 ccgcgaagta gcgcacgagc acgccgcgct gctccagcgc ctgctggagg tcgggcgccg
     1501 aggcatgcgc cggcaccgcc gcgagcacga agttgctctg gctctcggcg gccagcgcga
     1561 accccagctg ccggagcatc gcgctgaccc gtgcgcgctc gctgcgcacc cggcgccagg
     1621 tctcggcggc ctggtcgcgc gcggc
//
LOCUS       DASVOR010002491         1798 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_436142,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002491 DASVOR010000000
VERSION     DASVOR010002491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436142"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..1324)
                     /locus_tag="VF516_45775"
     CDS             complement(266..1324)
                     /locus_tag="VF516_45775"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase 3"
                     /protein_id="HEX8115146.1"
                     /translation="MIRTKILGLGSYVPERIVDNHELPFLNDRHVRQSTQQIETDDDW
                     IQKRTGIQARRFVPNDGTTATSDLALHAARRALADAGVEAAEIDCIIFGTLSPDFHFP
                     GTGVVLQRKLGIAERTSCACFDIRQQCSAFIYGLQMADAFIRAGTYRRILLVGAEIHS
                     HALDFSTRGRDVTVLFGDGAGAMVLGPTESDDPRAGVVYTAAYADGTGAMDLCLKIFE
                     IARLPYLDYDARDREQNRMMYPFMEGKRVYINAIRGMVMSTQSALARTGMTWDDIQWF
                     VPHQANLRINEKVAEVASIPRDKVLNTIEFYGNTTAATVPLTIDHWRQRGKVQRGDRI
                     LSSVFGSGFTWGAAIFTL"
     gene            1412..>1798
                     /locus_tag="VF516_45780"
     CDS             1412..>1798
                     /locus_tag="VF516_45780"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8115147.1"
                     /translation="MSASRSGQPWLTWCVALVTVALANEAQGAPSAAPPSQRGWPIYE
                     RLCLPCHGVRGDGHGPAAPYVWPAPRAFTRGEMKWRSVPVGQPASEDDVRATILLGAP
                     GTAMPAFAALTPDQQDDVIAVVRAFAP"
BASE COUNT          255 a          583 c          669 g          291 t
ORIGIN      
        1 gctgcaccgg cgagcccacc ggcgcgagtg cctgatcgac ccgcgtccgc ttgcccggcg
       61 catcgtgaac ggacgcagcc gacgcgccgc gggcgccgac gtcgggccga gcggtcttgc
      121 cagcgtgctc gctcattcgg ctcttgctac caggtttcgc ccggcgagct tccccgggtt
      181 ccgcgaacaa atgttaccgg cggagcgaaa cgctgcaacc ggggcgacct ctgtatgcct
      241 gcatgcagcg cgccgcggcg gtgactcaca acgtgaagat cgcggcgccc caggtgaagc
      301 ccgagccgaa caccgacgac aggatccggt cgccgcgctg gaccttgccg cgctggcgcc
      361 agtggtcgat ggtgagcggc acggtggcgg cggtggtgtt gccgtagaac tcgatcgtgt
      421 tgagcacctt gtcgcgcggg atgctggcga cctcggcgac cttctcgttg atccggaggt
      481 tggcctggtg ggggacgaac cactggatgt cgtcccaggt catgccggtc ctggcgagcg
      541 ccgactgggt cgacatcacc atgccgcgga tcgcgttgat gtagacccgc ttgccctcca
      601 tgaacggata catcatccgg ttctgctcgc gatcgcgggc gtcgtagtcg aggtacggca
      661 ggcgggcgat ctcgaagatc ttgaggcaga ggtccatcgc gccggtgccg tcggcgtacg
      721 ccgcggtgta gaccacgccg gcgcgcggat cgtcggactc ggtcgggccg agcaccatcg
      781 cgccggcgcc gtcgccgaac agcacggtga cgtcgcggcc gcgggtcgag aagtcgagcg
      841 cgtggctgtg gatctcggcg ccgaccagga ggatgcggcg ataggtgccg gcgcggatga
      901 acgcgtcggc catctgcaga ccgtagatga acgccgagca ctgctggcgg atgtcgaagc
      961 acgcgcagct ggtgcgctcg gcgatgccga gcttgcgctg gagcacgacg ccggtgcccg
     1021 ggaagtggaa gtcgggcgac agcgtgccga agatgatgca gtcgatctcg gcggcctcga
     1081 cccccgcgtc ggcgagcgcg cgccgggcgg cgtggagcgc gagatcgctg gtcgccgtcg
     1141 tgccgtcgtt cgggacgaag cggcgggcct ggatgccggt gcgcttctgg atccagtcgt
     1201 cgtcggtctc gatctgctgc gtggactgcc ggacgtggcg atcgttcagg aagggaagct
     1261 cgtggttgtc cacgatgcgc tccgggacat agctccccag cccgaggatc ttcgtgcgga
     1321 tcatatgacg tggatatgac atgaggctcc ttgacgacaa ggctcggagg cagatctggc
     1381 gcggcgtttt cgaatgcggc aggttcggtc cgtgagtgca tctcgatcgg ggcagccttg
     1441 gctcacgtgg tgcgtagcgc tcgtcaccgt cgcgctggcg aacgaggcgc agggcgctcc
     1501 gtccgcggcg ccgccgtcgc agcgcggctg gccgatctac gagcggctgt gcctgccctg
     1561 ccacggcgtt cgcggcgacg gtcatggtcc cgcggcgccg tacgtgtggc cggcaccccg
     1621 tgcgttcacg cgcggcgaga tgaagtggcg ctccgtaccg gtcgggcaac cggcgagcga
     1681 ggacgacgtg cgcgcgacga tcctgctcgg cgcgcccggc accgcgatgc cggcgttcgc
     1741 cgcgctgacg ccggaccagc aggacgacgt gatcgcggtc gtgcgcgcgt tcgccccc
//
LOCUS       DASVOR010002492        10663 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_173737,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002492 DASVOR010000000
VERSION     DASVOR010002492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 10663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10663
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173737"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VF516_45785"
     CDS             <1..775
                     /locus_tag="VF516_45785"
                     /inference="COORDINATES: protein motif:HMM:NF013601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HEX8115148.1"
                     /translation="AQPLAVPRTPGTLGFAGVEWVDPTTARDPQATVLAVAENLWHHF
                     EGPRALRAAERVFRFVDGRIDPAQAAELERLWHLLSRARVLTVTRALATGSLAINAHL
                     HERALDRMTVSGRPELVPGEPVMITANDYQRGLFNGDQGIIVRADEGLGRHHYRAVFR
                     IAGELRPFAIEALRDHLELAWALTVHKSQGSELDAVALILPHDDLPLVTRELIYTGIT
                     RARTGVAIVGARQLLVAGGKRRALRYSGLANRLRAETRP"
     gene            772..990
                     /locus_tag="VF516_45790"
     CDS             772..990
                     /locus_tag="VF516_45790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115149.1"
                     /translation="MTDRPPPGKGDPTCPHDERDVGVCVRCGHCVHELVLNGACYFCG
                     STDLDPVAMSPRPPRVIPPDHLIRKKPT"
     gene            1157..1750
                     /locus_tag="VF516_45795"
     CDS             1157..1750
                     /locus_tag="VF516_45795"
                     /inference="COORDINATES: protein motif:HMM:NF025431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4255 domain-containing protein"
                     /protein_id="HEX8115150.1"
                     /translation="MQVIGRVLVFLRKRLDDYLRAELGGAGEDPVADKVVFLDGDKMD
                     PISFQEGAVSELLINLEEERLLRAADPYVGVQDDGRPQRKHPDLRLVLYVLFAARFEQ
                     YDMAWDHLTKVIEYLQWNPAFDRGRNPDLPVGVDKLTFELVTQTFAEQNDIWNALRTT
                     YLPSALYRVRMVVVRDARPVLSEQITKPAVINIHRVS"
     gene            1771..3075
                     /locus_tag="VF516_45800"
     CDS             1771..3075
                     /locus_tag="VF516_45800"
                     /inference="COORDINATES: protein motif:HMM:NF016845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath C-terminal domain-containing
                     protein"
                     /protein_id="HEX8115151.1"
                     /translation="MRNTPGVYIQEVSTLPPSVAEVATAIPAFIGTTEKGPAGAVKVA
                     QINTLLEYEQTFGKPKPTAFSTTAVGAVKPPSSTLPETPLWYAVNLYFKNGGGRCYVV
                     SVDSHDARPSAARFQAGLDALATEDEPTLIVIPEAVGLAAADYSTVCQAALAHCQAMG
                     DRFAILDVKDGDVAAFRNGIGTSNLSYGAAYHPYLKTALPHAYDEAGVKVGEANDAPT
                     LASVKTSNTALYNDVKMQLGNQRVDLPPSAAMAGIYASIDRTRGVWKAPANVSVSAVI
                     GPAAQITNDQQDLLNVDPTSGKSVNAIRAFTGKGTIVWGARTLAGNDNEWRYVNVRRL
                     FIMIEESARKSTAFAVFEANDASTWLKVKGMLESYLYGLWEQGALQGSKPEAAYYVNV
                     GLGKTMTAQDVLEGRMNVEIGIAAVRPAEFIILKFSHKLPGA"
     gene            3085..3552
                     /locus_tag="VF516_45805"
     CDS             3085..3552
                     /locus_tag="VF516_45805"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8115152.1"
                     /translation="MSKDDIRTAYPLPVYNYRVEIAGAAVAFSEVSGLSIAYETSTYK
                     ESPTAAGAPGPRTMIVPGQRNPAKITLKKGIVRGVSIKTLFGWISTTTINQVEKKDIF
                     IRLCDENGAAVISWKVVNAFPTKLDAPTFDARSNDAAIESMELSADFVSLEEA"
     gene            3556..4002
                     /locus_tag="VF516_45810"
     CDS             3556..4002
                     /locus_tag="VF516_45810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8115153.1"
                     /translation="MAQTPQQQRTLHPLAAYNFQVTVDGVAMRFAKVSGLHREHQTVT
                     YRDGLSFQEGERITKFHVDAFTPVTLERGTVLGDKQLSDWLERSDPSAMEVSLCDETG
                     LPVIAWSIARAVPVKLTAPTFDASTNQLSIDSLEIRAAGISIKHLT"
     gene            4022..4465
                     /locus_tag="VF516_45815"
     CDS             4022..4465
                     /locus_tag="VF516_45815"
                     /inference="COORDINATES: protein motif:HMM:NF018543.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEX8115154.1"
                     /translation="MDLVLGSRFAAVFFIGGVVPNPIDIRFQRVSGLSATIETTAVNE
                     GGQNLYTHRLPRRVGYGNLTLERGFVIGSPLNIEFNLAMSLFQFAPSNVMITLLAADA
                     TPLAAWLFLKAYPVRWATADLSASEDKVLIDTLELSYTSMQALRV"
     gene            4468..4671
                     /locus_tag="VF516_45820"
     CDS             4468..4671
                     /locus_tag="VF516_45820"
                     /inference="COORDINATES: protein motif:HMM:NF039278.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5908 family protein"
                     /protein_id="HEX8115155.1"
                     /translation="MAIEIKQLIIRAVIEDARPRPTAAPASRAVPGPVHPAAPSEPGV
                     DRDALVAECTRHVLRELRKERGR"
     gene            4686..5435
                     /locus_tag="VF516_45825"
     CDS             4686..5435
                     /locus_tag="VF516_45825"
                     /inference="COORDINATES: protein motif:HMM:NF039279.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115156.1"
                     /translation="MALGDLFTLAKLTIEGYSDPQRSQPLGPPPFTVQYNPESFSLRH
                     ENVFHRPSRQANTAGEGGWSYSQPKRLSITLVLDGTNVGYMGAERLGSLPTVAGQISA
                     FLGICYHVQGQSHEPAYLKLSWGAALGGFAPLGGMQQPKFSCRLASVDVHYKAFDRDG
                     SPLHAELAAVFAEHLDPQLDAAQTKRTSPDLTHRRVVVDGDTLPLLCREIYGSSVHYL
                     RVAQVNRLDDFRNLTAGQELIFPPFERPRKG"
     gene            5437..7071
                     /gene="vgrG"
                     /locus_tag="VF516_45830"
     CDS             5437..7071
                     /gene="vgrG"
                     /locus_tag="VF516_45830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HEX8115157.1"
                     /translation="MGVATITITSEGTPLDPAVGILAVEVRRELNRVPEATITVVDGS
                     VAERRFAHSDTGFFEPGKSLAIAVRDGDGVDTTLFEGLVVRHAVESRPGTSSLRVELK
                     DAAFKLTRQRKTAVFRKQADDDAIQKLIGDAGLQVGELASTQPQHEELIQFYASDWDF
                     IVSRADAQGLVVDVDRGTISVQAMVSDAQPRATLEHGMDDVELGLEIDAGEQWAAMTG
                     QAWDVQQQAVTAPADARSPGVTVGNLDAAAIARKLGGDSYALIHPGLVVQDELTAWAT
                     SRLTRSRLAMLRGCASIPGTPDLAPRDIVTLAGVGDRFNGDAYVSGVPHTIDHNGWRT
                     ELQLGLPPEPFARQPDIADLPAAGLLPPIHGLHVGVVDDFESDPLGEHRIKVLLSMLD
                     HQQGAVFARVARPDAGDQRGLVFWPEPGDEVVVGFVNGDPRQAVVLGALHGSTNAPPD
                     AAGPPSDDNTRRAIVSKAGTVIAFDDDKASVRIETPAANKVVIDDDAESITLADQHGN
                     TITLDKNGITLRSAKDFTIDAASGKVVIQGKTVDVK"
     gene            7068..7283
                     /locus_tag="VF516_45835"
     CDS             7068..7283
                     /locus_tag="VF516_45835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115158.1"
                     /translation="MTQPRGQHHAMKNLQLELTIDETNLILEALGALPFARVYTLIGR
                     IQESARKQIHDPPVDHGASGADREHAR"
     gene            7280..7696
                     /locus_tag="VF516_45840"
     CDS             7280..7696
                     /locus_tag="VF516_45840"
                     /inference="COORDINATES: protein motif:HMM:NF016829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HEX8115159.1"
                     /translation="MTAPATSFLGTGWSFPPAFSPGGAELAMVSDTDDVHQSLQILLA
                     TRQGERAMQDSFGCNLDSMMFEAMNQSLVDHVTSLIYDAILRHEPRVDLRAIDVTPTD
                     AAGVLQIRIDYAIRETNSRYNMVFPFYLNEAIARVA"
     gene            7696..8124
                     /locus_tag="VF516_45845"
     CDS             7696..8124
                     /locus_tag="VF516_45845"
                     /inference="COORDINATES: protein motif:HMM:NF039577.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115160.1"
                     /translation="MADYILIDGDKAMFTPSFGAATVVVQPGTLTASGPATITGKNMC
                     VSGDENSVSVQGCTYMTASHTIPGTGTLEIASLAGDQTATRTRSGDTLVLLVGSSFTA
                     SFSVQSPAQQPTSAGPVPDPTPKYSGTGSFVTTNTRFKGV"
     gene            8125..>10663
                     /locus_tag="VF516_45850"
     CDS             8125..>10663
                     /locus_tag="VF516_45850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115161.1"
                     /translation="MSRRSTPLSPRSRDGTSQRSRRLPALDPAYAPIDDRTSADLLGF
                     VRAYADRLWFPEADPETGELRADKTWLGLVRHADTATGAGDLSIADIVAYVADPTRFA
                     GEQARWLGRPHFALLLAFVELLGHARDLLNGLTRRHLDHYYREVLQLRAEPPTPDRAM
                     VVIGLSPGAAEVRLPAGTELQAGRDAGGPLIYRTEREIVANRASVAQLRSVFVDRRIT
                     GIPDVRADRSLTAADALTGTLRIALGSPQPGDDIPRFDGKPIDAAFLLGLRGLLDFAG
                     KPLHLEHDELRSLIRLYHRRRDADADWAQVNRLLGVANPARPRDFAANLFSRVGKLDF
                     KTDGLPQVDTVDDLALYAGEPDVRRYIDSKLAAIGFDRFLQAMQIKRASDADWAEILR
                     LLERIGQRQHEQRAWTLPPGAPTASGDPIDFGASLQAALGNAWPPPWPAGTTGIEDYD
                     ALLRRLEAHLSMPVEPIATLVAFAEELGHDAQSERHDWTAVDRILADAHREKLRAARQ
                     AELAAARGGQDVAAFDREASFVLGTTTPLSWTAAQPQLAAHIDKPALTQLAQFRDGLA
                     AGQPISGFSWSDADRLFERAWHHVLQLPDPVAQRIELHNVHAHDDATQVRDRAAAPGW
                     KTFGARPATADDQHPQGATLGWALRTPLLALGEGTRTLILTLGLRGEHFGKGEQDDFL
                     RALGLVPADPADRTEARLIAALQHALAIEVSTARGWIALDLTRARLAGGAPGDDYWSV
                     RGVTRPIADDRPALQLQAVIARSVDPLAPIKGEAAIWPALRLSLRPQWDAVAREWRTA
                     IEALAPLVLAAVHLAVGVDGLAALHLQQDDREIDPRKPFEPFGSRPAV"
BASE COUNT         1690 a         3916 c         3605 g         1452 t
ORIGIN      
        1 cgcccagccg ctcgccgtgc cgcgcacgcc gggcacgctc gggttcgccg gcgtcgagtg
       61 ggtcgacccc accaccgccc gcgatccgca ggccaccgtg ctcgccgtcg ccgagaacct
      121 gtggcaccac ttcgagggcc cgcgcgcgct gcgcgccgcc gagcgcgtgt tccggttcgt
      181 cgacggccgg atcgatccgg cgcaggcggc cgagctcgag cggctgtggc acctgctgtc
      241 gcgcgcccgc gtgctcacgg tgacccgcgc gctggcgact ggctcgctcg cgatcaacgc
      301 ccacctccac gagcgcgcgc tcgaccggat gacggtgtcc ggccgccccg agctggtccc
      361 cggcgagccg gtgatgatca ccgccaacga ctaccagcgc ggcctgttca acggcgacca
      421 gggcatcatc gtgcgcgccg acgagggcct cggccgccac cactaccgcg cggtgttccg
      481 gatcgccggc gagctgcggc cgttcgcgat cgaggcgctg cgcgaccacc tcgagctggc
      541 gtgggcgctg accgtgcaca agagccaggg cagcgagctc gacgccgtcg cgctgatcct
      601 gccgcacgac gacctcccgc tggtcacccg cgagctgatc tacaccggca tcacccgggc
      661 ccggaccggc gtcgcgatcg tcggtgcgcg ccagctgctg gtcgcgggcg gcaagcgccg
      721 cgcgctgcgc tactcgggcc tggccaaccg gctgcgcgcg gagacccggc cgtgaccgac
      781 cgcccgccgc ccggcaaggg cgatccgacc tgcccgcacg acgagcgcga cgtcggcgtc
      841 tgcgtccgct gcggccactg cgtccacgag ctggtgctca acggcgcctg ctacttctgc
      901 ggctccaccg atctcgatcc ggtcgcgatg tcgcccaggc cgccccgggt catcccgccc
      961 gatcacctga tccggaagaa gccgacctga ccgaaaattc attggccatg taatccgtcc
     1021 gagctctgcg gtgcagcaga tacagcagtg ctcgcgggcc actacatctg tgtgtatatc
     1081 cagacgattc gagtggcctc ctgtgttcat gcttgacatt accgatcgag taaggtctcc
     1141 tggtggaaac gggagggtgc aggtgatcgg gagggtgctc gtcttccttc gaaagcggct
     1201 ggacgactat ctgcgtgccg agctcggcgg cgcaggggag gatccggtcg ctgacaaggt
     1261 cgtcttcctc gacggtgaca agatggaccc gatcagcttc caggagggag ctgtctcgga
     1321 gctgctgatc aacctggagg aggaacggct gctgcgcgcc gccgatccgt acgtcggcgt
     1381 ccaggacgac gggaggccgc agcgcaagca ccccgacctg cggctggtcc tctatgtgct
     1441 gtttgcggcg cgattcgagc aatacgacat ggcctgggat cacctgacca aggtcatcga
     1501 atacctgcaa tggaatccgg cattcgaccg gggtcgcaac ccggatttgc cggtcggggt
     1561 cgacaagctg acattcgagc tcgtgaccca gaccttcgca gagcagaacg acatctggaa
     1621 cgcgctgcga acgacctacc tgccatcagc gctctaccgg gtccgcatgg tcgtcgtgcg
     1681 cgatgccagg ccggtcctga gcgagcagat caccaagccc gccgtcatca acatccaccg
     1741 cgtgtcctga cctccaccac cacgagaccc atgcgcaaca ctcccggcgt atacattcaa
     1801 gaagtctcga cgctgccgcc ctcggttgcc gaggtcgcga ccgcgattcc agcgttcatc
     1861 ggcaccaccg agaagggccc ggccggggcc gtcaaggtgg cgcagatcaa cacgttgctg
     1921 gaatacgagc agaccttcgg caagcccaag ccgaccgcgt tctcgaccac ggccgtgggc
     1981 gcggtcaagc cgccgagctc gaccctgccc gagacgccgc tgtggtacgc ggtgaacctg
     2041 tacttcaaga acggcggcgg ccggtgctac gtggtgtcgg tcgacagcca cgacgccagg
     2101 cccagcgcgg cacggttcca ggccgggctc gacgcgctcg cgaccgagga cgagccgacg
     2161 ctgatcgtga tcccggaggc ggtcggcctg gctgcggcgg actacagcac ggtgtgccag
     2221 gccgcgctcg cccattgcca ggcgatgggc gaccggttcg ccatcctgga tgtcaaggac
     2281 ggcgacgtgg ccgcgttccg caacgggatc ggcaccagca acctgtcgta cggagccgcc
     2341 tatcacccgt acctcaagac cgcgctgccg cacgcgtacg acgaggccgg cgtcaaggtg
     2401 ggtgaggcca acgacgcacc gaccctggcc tcggtgaaga ccagcaacac cgcgctgtac
     2461 aacgacgtca agatgcagct cgggaaccag cgggtcgacc tcccgccgag cgccgcgatg
     2521 gcggggatct acgccagcat cgaccgcacc cgcggggtgt ggaaggcccc cgccaacgtg
     2581 tcggtgtccg cggtgatcgg tccggcggcc cagatcacca acgaccagca ggacctgctc
     2641 aacgtcgatc cgacgtcggg caagtcggtc aacgcgatcc gcgcgttcac cggcaagggc
     2701 acgatcgtgt ggggcgcccg caccctggcc ggcaacgaca acgagtggcg ttacgtgaac
     2761 gtccgccgcc tgttcatcat gatcgaggag tccgcgagga agtcgaccgc gttcgccgtg
     2821 ttcgaggcca acgacgcatc gacctggctc aaggtcaagg gcatgctgga gagctacctc
     2881 tacggcctgt gggagcaggg cgcgctgcag ggttcgaagc cggaggccgc gtactacgtc
     2941 aacgtcggcc tcggcaagac catgacggcg caggacgtgc tcgaaggccg gatgaacgtc
     3001 gagatcggca tcgcggccgt gcgccctgcc gagttcatca tcctcaagtt ctctcacaag
     3061 ctgccggggg cctgaaccat ggcgttgagc aaggacgata tccgcactgc ctatccactc
     3121 cccgtctaca actaccgggt cgagatcgcc ggcgccgccg tcgcgttctc ggaggtctcg
     3181 gggctgagca tcgcctacga gaccagcacc tacaaggaga gcccgaccgc cgccggcgcc
     3241 cccggcccgc gcaccatgat cgtcccgggg cagcgcaacc cggcgaagat cacgctcaag
     3301 aagggcatcg tccgcggggt gagcatcaag accctgttcg gctggatctc gacgaccacg
     3361 atcaaccagg tcgaaaagaa ggacatcttc atccggctgt gcgacgagaa cggcgccgcg
     3421 gtgatctcgt ggaaggtggt caacgcgttc cccaccaagc tcgacgcgcc gaccttcgac
     3481 gccaggtcga acgacgccgc gatcgagagc atggagctgt cggccgactt cgtctcgctc
     3541 gaggaagcgt gatccgtggc gcagaccccc cagcagcaac gcacgctcca cccgctggcc
     3601 gcctacaact tccaggtaac ggtggacggc gtggcgatgc ggttcgccaa ggtgtcgggt
     3661 ctgcatcgcg agcaccagac cgtgacctat cgcgacgggc tgagcttcca ggagggcgag
     3721 cgcatcacca agttccacgt cgacgcgttc accccggtca cgctggagcg cggcaccgtg
     3781 ctcggcgaca agcagctgtc cgactggctg gagcgctccg acccgagcgc gatggaggtc
     3841 agcctgtgcg acgagaccgg gctgccggtg atcgcgtgga gcatcgcccg ggcggtgccg
     3901 gtcaagctca ccgcgccgac cttcgacgcc tcgaccaacc agctcagcat cgacagcctg
     3961 gagatccgcg cggccgggat ctcgatcaaa cacctgacgt gacggagggg cacgacctgc
     4021 catggatctc gtcctcggct cacgattcgc ggcggtgttc ttcatcgggg gcgtcgtgcc
     4081 caacccgatc gacatccggt tccagcgggt gtccggactg tcggcgacga tcgagaccac
     4141 cgcggtcaac gagggcggcc agaacctgta cacccaccgg ctgccccggc gggtcggcta
     4201 cggcaacctc acgctcgagc gcggcttcgt gatcggctcg ccgctcaaca tcgagttcaa
     4261 cctggcgatg agcctgttcc agttcgcgcc gtccaacgtg atgatcacgc tgctcgccgc
     4321 cgacgcgaca ccgctggcgg cctggctgtt cctcaaggcg tacccggtgc ggtgggcgac
     4381 cgccgatctc agcgccagcg aggacaaggt gctgatcgac acgctcgagc tgtcctacac
     4441 gagcatgcag gcgctgaggg tgtgatcatg gcgatcgaga tcaagcagct gatcatccgc
     4501 gcggtcatcg aggacgcccg cccgcgtccc accgccgcgc ccgcgagccg cgcggtgccc
     4561 gggccggtcc acccggcggc cccgtccgag cccggcgtgg accgcgacgc cctggtcgcc
     4621 gagtgcacgc gccacgtgct gcgcgagctg cgaaaggagc ggggccgctg acgccccggt
     4681 gagccatggc gctcggcgac ctgttcacgc tggccaagct gacgatcgag ggctacagcg
     4741 atccccagcg ctcgcagccg ctcgggccgc cgccgttcac cgtccagtac aacccggagt
     4801 cgttctcgct gcgccacgag aacgtgttcc atcggccgtc gagacaggcc aacaccgccg
     4861 gcgagggcgg ctggtcgtac agccagccca agcggctctc gatcaccctg gtgctcgacg
     4921 gcaccaacgt cggctacatg ggcgcggagc ggctcggcag cctgccgacg gtggccggtc
     4981 agatcagcgc gttcctgggt atctgctacc acgtccaggg ccaaagccac gagccggcgt
     5041 acctcaagct gtcgtggggc gccgcgctcg gcgggttcgc tccactgggc ggcatgcagc
     5101 agccgaagtt ctcgtgccgg ctggcgtcgg tcgacgtcca ctacaaggcg ttcgatcgcg
     5161 acggctcgcc gctccacgcc gagctcgcgg ccgtgttcgc cgagcacctg gatccccagc
     5221 tcgacgcggc ccagaccaag cggacgtcgc cggacctgac ccaccgccgg gtggtcgtcg
     5281 acggcgacac cctgccgctg ctgtgccgcg agatctacgg ctcgtcggtc cactacctgc
     5341 gggtcgcgca ggtcaaccgg ctcgacgact tccgcaacct gaccgccggc caggagctga
     5401 tcttcccccc gttcgagcgg ccgcggaagg gctgacatgg gcgtcgcgac gatcacgatc
     5461 acgagcgagg ggacgccgct cgatcccgcc gtcgggatcc tggcggtcga ggtccggcgc
     5521 gagctcaacc gcgttcccga ggccacgatc accgtggtcg acggcagcgt ggccgagcgc
     5581 aggttcgcgc acagcgacac cgggttcttc gagccaggca agtcgctcgc gatcgcagtg
     5641 cgcgacggcg atggcgtgga tacgacgctg ttcgagggcc tcgtggtccg gcacgccgtc
     5701 gagtcgcggc ccggcacctc cagcctgcgg gtcgagctca aggacgccgc attcaagctg
     5761 acccggcagc gcaagaccgc ggtgttccgc aagcaggccg acgacgacgc gatccagaag
     5821 ctgatcggcg acgccggcct gcaggtcggc gagctcgcgt cgacccagcc ccagcacgag
     5881 gagctgatcc agttctacgc cagcgactgg gacttcatcg tgtcgcgcgc cgacgcccag
     5941 gggctggtcg tcgacgtcga ccgcggcacg atctcggtgc aggccatggt ctcggacgcc
     6001 cagcccaggg cgacgctgga gcacggcatg gacgacgtcg agctcgggct cgagatcgac
     6061 gccggcgagc agtgggcggc gatgaccggc caggcgtggg acgtccagca gcaggcggtg
     6121 accgcgcccg ccgatgcccg gtcgcccggc gtcacggtgg gaaacctcga cgccgcggcg
     6181 atcgcccgca agctgggcgg cgacagctac gcgctgatcc accccggcct ggtggtccag
     6241 gacgagctca ccgcgtgggc gaccagccgg ctgacccgga gccggctcgc catgctgcgc
     6301 ggctgcgcgt cgatccccgg cacccccgat ctcgcgccgc gcgacatcgt gacgctggcc
     6361 ggcgtgggcg accggttcaa cggcgacgcc tacgtctcgg gggtgcccca caccatcgac
     6421 cacaacggct ggcgcaccga gctgcagctc ggcctgccgc cggagccgtt cgcgcgccag
     6481 cccgacatcg ccgaccttcc ggcggccggg ctgctgccgc cgatccacgg cctgcacgtc
     6541 ggggtggtcg acgacttcga gtcggatccg ctcggcgagc accgcatcaa ggtgctgctg
     6601 tcgatgctcg accaccagca gggcgcggtg ttcgcccgcg tcgcgcgccc cgacgccggc
     6661 gaccagcgcg gcctggtgtt ctggcccgag cccggcgatg aggtcgtcgt cggcttcgtc
     6721 aacggcgacc cgcgccaggc ggtcgtcctg ggcgcgctgc acggctcgac caacgcgccg
     6781 cccgacgccg ccggcccgcc gagcgacgac aacacccggc gcgcgatcgt gagcaaggcc
     6841 ggcacggtga tcgccttcga cgacgacaag gcgtcggtgc ggatcgagac cccggccgcc
     6901 aacaaggtcg tgatcgacga cgacgccgag tcgatcacgc tcgccgacca gcacggcaac
     6961 acgatcaccc tcgacaagaa cgggatcacg ctcaggagcg ccaaggactt cacgatcgac
     7021 gccgcgtccg gaaaggtcgt gatccagggc aagacggtgg acgtgaaatg acgcaacccc
     7081 gaggccagca ccacgccatg aagaacctcc agctcgagct caccatcgac gagaccaacc
     7141 tgatcctcga ggcgctcggc gcgctgccgt tcgcccgggt ctacacgctg atcggccgga
     7201 tccaggagtc cgcccgcaag cagatccacg accctccggt cgaccacggc gccagcggcg
     7261 cggatcgcga gcacgcgcga tgacggcacc ggccacgagc ttcctcggca ccgggtggag
     7321 cttcccgccc gcattcagcc cgggcggcgc cgagctcgcg atggtgagtg acaccgacga
     7381 cgtccaccag tcgctgcaga tcctgctcgc cacccgccag ggcgagcggg cgatgcagga
     7441 cagcttcggc tgcaacctcg acagcatgat gttcgaggcg atgaaccagt cgctggtcga
     7501 ccacgtgacc agcctgatct acgacgcgat cctccggcac gagccgcgcg tcgacctgcg
     7561 cgcgatcgac gtgaccccga ccgacgccgc cggcgtgttg cagatccgga tcgactacgc
     7621 gatccgggag accaacagcc gctacaacat ggtgtttccg ttctacctca acgaggcgat
     7681 cgcccgggtg gcgtgatggc cgactacatc ctgatcgacg gcgacaaggc gatgttcacc
     7741 ccgagcttcg gggccgccac cgtcgtggtc cagcccggca cgctgacggc gagcggcccg
     7801 gcgacgatca ccggcaagaa catgtgcgtg tccggcgacg agaacagcgt gtcggtccag
     7861 ggctgcacct acatgacggc gtcgcacacg atcccgggca ccgggacgct ggagatcgcg
     7921 tcgctggccg gcgatcagac cgcgaccagg acccggagcg gtgacacgct ggtgctgctg
     7981 gtcggcagca gcttcaccgc gagcttctcg gtgcagagcc cggcccagca gccgacctcc
     8041 gccggcccgg tccccgatcc gacgccgaag tactcgggga ccggcagctt cgtgaccacc
     8101 aacaccaggt tcaagggagt gtgagtgtcc aggcgctcga ccccgctctc gccccgcagc
     8161 cgcgacggca ccagccagcg ctcgcggcgg ctgccggcgc tcgatccggc ctacgcgccg
     8221 atcgacgacc ggaccagcgc cgatctgctc ggcttcgtgc gggcgtacgc cgatcggctg
     8281 tggtttcccg aggccgatcc cgagaccggt gagctgcgcg ccgacaagac gtggctcggc
     8341 ctcgtccggc acgcggacac cgcgaccggc gccggcgacc tctcgatcgc cgacatcgtg
     8401 gcgtacgtgg cggatccgac gcggttcgcc ggcgagcagg cgcgctggct ggggcggccg
     8461 cacttcgcgc tgctgctggc gttcgtcgag ctgctcggtc acgcgcgcga tttgctcaac
     8521 ggcctgaccc gccgccacct cgaccactac taccgcgagg tcctgcagct ccgggccgag
     8581 ccgccgacgc ccgaccgcgc gatggtcgtg atcgggctgt cgcccggcgc ggccgaggtc
     8641 cggctgcccg cgggcaccga gctccaggcc ggacgcgacg cgggcggccc gctgatctac
     8701 cgcaccgagc gcgagatcgt cgccaaccgc gcgtccgtcg cccagctccg ctcggtgttc
     8761 gtcgaccggc ggatcaccgg catccccgac gtccgggccg accgcagcct caccgccgcc
     8821 gacgcgctga ccgggacgct gcggatcgcg ctgggctcgc cgcagccggg cgacgacatc
     8881 cctcggttcg acggcaaacc gatcgacgcc gcgttcctgc tcggcctccg ggggctgctc
     8941 gacttcgccg gcaagccgct ccacctcgag cacgacgagc tccgctcgct gatccggctg
     9001 taccaccggc ggcgcgacgc cgacgccgac tgggcgcagg tcaaccggct gctgggcgtc
     9061 gcgaaccccg cccggccgcg cgacttcgcg gccaacctgt tctcgcgggt cggcaagctc
     9121 gacttcaaga ccgacggcct gccgcaggtc gacaccgtcg acgacctcgc cctgtacgcg
     9181 ggcgagcccg acgtgcgcag gtacatcgac agcaagctcg ccgcgatcgg gttcgaccgc
     9241 ttcctccagg cgatgcagat caagcgcgcc agtgacgccg actgggccga gatcctccgg
     9301 ctgctcgagc ggatcggcca gcggcagcat gagcagcgcg cgtggacgct tccgcccggc
     9361 gctccgaccg cgtccggcga cccgatcgac ttcggcgcga gccttcaggc ggcgctgggc
     9421 aacgcctggc cgccgccgtg gccggccggc accacgggca tcgaggacta cgacgccctg
     9481 ctgcgccggc tggaggccca cctctcgatg ccggtcgaac cgatcgcgac cctggtcgcg
     9541 ttcgccgagg agctcggcca cgacgcccag agcgagcgcc acgactggac cgcggtcgac
     9601 cggatcctgg ccgatgccca ccgcgagaag ctgcgcgccg cgcgccaggc cgagctcgcc
     9661 gccgcccgcg gcgggcagga cgtcgcggcg ttcgaccggg aagcgagctt cgtgctcggc
     9721 accaccacgc cgctgtcgtg gaccgccgcc cagccgcagc tcgcggcgca catcgacaaa
     9781 ccggccctga cccagctcgc gcagtttcgc gacggtctgg ccgccggaca gccgatctcc
     9841 ggcttcagct ggagcgacgc cgaccggctg ttcgagcgcg cgtggcatca cgtcctgcag
     9901 ctgcccgatc cggtggcgca gcggatcgag ctccacaacg tccacgccca cgacgacgcg
     9961 acccaggtcc gggaccgcgc ggccgccccg ggctggaaga cgttcggcgc ccggccggcc
    10021 accgccgacg accagcaccc gcagggcgcg acgctgggct gggcgctgcg cacgccgctg
    10081 ctggcgctcg gcgagggcac gcgcacgctg atcctgacgc tgggactgcg cggcgagcac
    10141 ttcggcaagg gcgagcagga cgacttcctg agggcgctgg gccttgtccc cgccgacccc
    10201 gccgacagga ccgaggccag gctgatcgcc gcgctccagc acgcgctggc gatcgaggtc
    10261 agcaccgcca ggggctggat cgcgctcgac ctcacccggg cgcggctcgc cggcggcgcg
    10321 ccgggcgatg actactggtc cgtgcgcggc gtgacgcggc cgatcgccga cgatcgcccg
    10381 gcgctgcagc tccaggccgt gatcgcccgg agcgtcgatc cgctcgcccc gatcaagggc
    10441 gaggccgcga tctggccggc gctgcggctg tcgttgcggc cgcagtggga cgccgtggcg
    10501 cgcgagtggc gcacggcgat cgaggcgctc gcgccgctcg tgctcgccgc ggtccacctc
    10561 gcggtgggcg tcgacgggct ggccgcgctc cacctgcagc aggacgaccg cgagatcgat
    10621 ccgcgcaagc cgttcgagcc gttcggcagc cgccccgcgg tgg
//
LOCUS       DASVOR010002493         3404 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_232278,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002493 DASVOR010000000
VERSION     DASVOR010002493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232278"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..784)
                     /locus_tag="VF516_45855"
     CDS             complement(<1..784)
                     /locus_tag="VF516_45855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115162.1"
                     /translation="MQAVLQRVLPVVLVLATSTAAFADSRILRADVDLGEGLLFINGQ
                     DLPRHEPTVVLGSTRLHVLSFSRTDIVAQVPSGLAPATYLLLVGDVLHFAVSVGDTGP
                     QGPQGPKGDTGPQGPQGPQGPAGPVGPQGPQGLKGDTGPQGPQGLKGDTGPQGPQGLK
                     GDTGPQGPQGLKGDTGPQGPQGLKGDTGPQGPQGDVGPQGPIGPIGPIGPAGPIGPPG
                     PQGLTGPIGPAGPIGPPGLQGLTGPIGPAGPIGPPGLQGLTGP"
     gene            1129..2253
                     /locus_tag="VF516_45860"
     CDS             1129..2253
                     /locus_tag="VF516_45860"
                     /inference="COORDINATES: protein motif:HMM:NF012998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HEX8115163.1"
                     /translation="MPEPDLASLRDRIEAVDRQLIALVAERLKIVEDVAAAKLAAASP
                     FRDREREDLLLQRLRGLAIAAGLDAHQIERLYRVIMDMSVAHQEATVRNRPDAPLRIS
                     YQGVEGSYSHIAAQRRYAGRPGGALLTGHDTFRAAAEAVASGTSDLALLPIENTTAGS
                     INETYDLLAVGSLHITGEVVSAIEHCLLALPGVTLDELRTVISHPQALAQCHDLFARQ
                     PHLTPRADLDTAGAARRVAEANDRTLGAIASSAAARRYGLAVVASGIQSAAGNATRFV
                     EIALRPAPVPEGVTAKTSLVFALADRPGVLGEILQRLSDRNLSLTKLESRPIPEVPFT
                     YRFYADVLGHAASEPFHAALQDIRPLTTDLRVLGTYPSAV"
     gene            2261..3235
                     /locus_tag="VF516_45865"
     CDS             2261..3235
                     /locus_tag="VF516_45865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HEX8115164.1"
                     /translation="MRAVRVHELIGPSGLRIDELPDPSVGAGQVLIDVRASGVNFPDV
                     LLSRGLYQFKPTLPFSPGAECAGVVREVGAGVTRVAPGDRVALTQVNGTFVDKLVAAE
                     ANVVKLPDAVSFEVGAATQMTYLTTYHALVDRAAIQPGETLLVLGAAGGVGAAACELG
                     AALGAKVIAAASTDDKLAFCREHGASEVINYAREDLKDRIKALTSGNGVNVVYDPIGG
                     AYAEPALRGIAWQGRYLVIGFAAGEIPKIPLNLVLLKGCQIVGVFWGAFAMRDPARNR
                     EHANQVFQWVAEGKLRPAVDAALPFERAGEALERLEKRQVKGKLVLVP"
BASE COUNT          493 a         1216 c         1214 g          481 t
ORIGIN      
        1 cagggccggt aagtccttgc agacctggcg gaccgatggg acccgcagga ccgatggggc
       61 cggtcagtcc ttgcagacct ggcggaccga tgggacccgc aggaccgatg gggccggtga
      121 gtccttgcgg acctggcgga ccgatggggc ccgcggggcc gatcggtcca atagggccga
      181 tgggaccctg cgggccaacg tcgccctggg gcccctgcgg ccccgtatcg cctttgagtc
      241 cttgcgggcc ttgcgggccc gtatcgcctt tgaggccctg cggaccttgc gggccggtgt
      301 cgcctttgag gccctgcgga ccttgcgggc cggtatcgcc cttgaggccc tgcggacctt
      361 gtgggccggt gtcgcccttg aggccctgcg gtccctgcgg accgacgggc ccggccggac
      421 cctgcggacc ctgcggaccc tgcgggccgg tgtcgccctt ggggccctgc ggaccctgcg
      481 gcccggtatc acccaccgag acggcgaagt ggagaacgtc tccgaccagc aaaagatacg
      541 tcgcgggcgc gagcccggac gggacctggg ccacgatatc ggtccgcgaa aagctcaata
      601 cgtgcagtcg cgtactgccc agtacgaccg tgggctcgtg tctggggagg tcctggccgt
      661 tgatgaacag caatccttcg ccgagatcga cgtcggcgcg caggatgcgg gagtcggcga
      721 acgccgcggt gctcgtggcg agcaccagca cgacgggcag aacgcgttgc agcacggctt
      781 gcatggctct cctccgctcg atccgaggca gtctgacgcc gcataccagc agactccgca
      841 gcacagcgtc aactggaatt tcttacgaac tgaataacta catctcccta tctcgaactg
      901 cgtattcatg cgcctggtga tcgaaggtag tcgcgagtca gccagatccc gaacgcgaga
      961 gtcggccggc agcggggtcg ctgcggggcg cagcaatctg cgccgcgctc gccgagcgcc
     1021 tgggcgcggc cgccacaggg atggcgaccg gcgaaggacg gcaaccgcac ggtttccgca
     1081 gagggcgggt ggaacccgtc gacaggacgg gggaaagtag gccccgtcat gccggaacct
     1141 gatctcgcat cgctccgcga ccgcatcgag gcggtcgatc gccagctgat cgcgctcgtc
     1201 gccgagcggc tgaagatcgt cgaggacgtc gccgccgcca agctggcggc cgcgagcccg
     1261 ttccgcgacc gcgagcgcga ggacctgctg ctccagcggc tgcgcgggct ggcgatcgcc
     1321 gcggggctgg acgcccacca gatcgagcgc ttgtaccgcg tgatcatgga catgtcggtc
     1381 gcgcaccagg aggcgacggt gcgcaaccgc cccgatgcgc cgctccggat ctcgtaccag
     1441 ggcgtcgagg gcagctacag ccacatcgcg gcgcagcgcc gctacgccgg gcgcccgggg
     1501 ggcgcgctgc tcaccgggca cgacacgttc cgcgccgcgg ccgaggcggt ggcctcgggc
     1561 acctccgacc tggcgctgct gccgatcgag aacaccaccg cgggcagcat caacgagacc
     1621 tacgacctgc tcgcggtggg ctcgctgcac atcaccggcg aggtggtgtc ggcgatcgag
     1681 cactgcctgc tcgcgctgcc cggggtgacg ctggacgagc tgcgcaccgt gatctcgcac
     1741 ccgcaggcgc tcgcccagtg ccacgacctg ttcgcccggc agccccacct gacgccgcgc
     1801 gccgacctcg ataccgcggg ggccgcgcgc cgggtcgccg aggccaacga ccggacgctc
     1861 ggggcgatcg cgagctccgc cgccgcccgg cgctacgggc tggcggtggt cgccagcggc
     1921 atccagtcgg cggcgggcaa cgcgacccgg ttcgtcgaga tcgcgctgcg gcccgcgccg
     1981 gtgcccgagg gcgtcaccgc caagacctcg ctggtgttcg cgctcgccga ccggccgggc
     2041 gtgctcggcg agatcctgca gcggctgtcg gaccgcaacc tctcgctcac caagctggag
     2101 tcgcggccga tccccgaggt gccgttcacc taccggttct acgccgacgt gctcggccac
     2161 gccgccagcg agccgttcca cgccgcgctc caagacatcc ggccgctgac aaccgacctc
     2221 cgcgtgctcg gcacgtaccc gtccgctgta taaccgcggc atgcgcgcag ttcgagtcca
     2281 cgagctcatc ggtccgtccg gcctccggat cgacgagctt cccgatccca gcgtcggcgc
     2341 cggccaggtc ctgatcgacg tccgcgccag cggcgtgaac ttccccgacg tgctcctgtc
     2401 gcgggggctg taccagttca agccgacgct gccgttctcg cccggtgccg agtgcgccgg
     2461 cgtggtgcgc gaggtcggcg ccggggtcac ccgggtcgcg cccggcgacc gcgtggcgct
     2521 gacccaggtc aacggcacgt tcgtcgacaa gctggtcgcc gccgaggcca acgtcgtcaa
     2581 gctgcccgac gcggtcagct tcgaggtcgg cgccgcgacg cagatgacgt acctcacgac
     2641 ctaccacgcg ctggtcgacc gcgcggcgat ccagcccggt gagaccctgc tcgtgctcgg
     2701 cgcggccggc ggcgtcgggg ccgcggcctg cgagctcggc gcggcgctcg gcgccaaggt
     2761 gatcgctgcg gcgtcgaccg acgacaagct cgcgttctgc cgcgagcacg gcgcgagcga
     2821 ggtcatcaac tacgcgcgcg aggacctcaa ggaccggatc aaggcgctga cctcgggcaa
     2881 cggcgtcaac gtggtctacg acccgatcgg cggcgcgtac gccgagcccg cgctgcgcgg
     2941 catcgcgtgg cagggccgtt acctcgtgat cggcttcgcc gccggcgaga tccccaagat
     3001 cccgctcaac ctggtgctgc tcaagggctg ccagatcgtc ggggtgttct ggggagcatt
     3061 cgcgatgcgg gatccggcgc gcaaccgcga gcacgccaac caggtcttcc agtgggtcgc
     3121 cgagggcaag ctgcgcccgg cggtcgacgc cgcgctgccg ttcgaacgcg cgggcgaggc
     3181 cctggagcgg ctggagaagc gccaggtcaa gggcaagctc gtgctcgtgc cgtaggccgg
     3241 acaatccggg aagccttgtc tccggcgacg cgggcaagtc gttcgtgatg ccggattcgt
     3301 agccagccgt ctcaagctgt tggcgagctg gtcgagcgat gggcggcaca gctcggcgcg
     3361 gcgaccgtcg acatcgtggc gaccgcagtg gacggcgccg cgcc
//
LOCUS       DASVOR010002494         3227 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_524020,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002494 DASVOR010000000
VERSION     DASVOR010002494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524020"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            298..2466
                     /locus_tag="VF516_45870"
     CDS             298..2466
                     /locus_tag="VF516_45870"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEX8115165.1"
                     /translation="MMTSPRDTTRRYRAFLSYSHAADDRFAEALQKALKQFARPLFAV
                     RALNIFRDRTDLSATPELWNSVRSALDDSDYLIYLASPKAAGSRWVKRELDHWIRTRS
                     TLATLLIVLTDGELKWEGADFDWSQTTSLPRLLDFPELAGEAPLSLEQRFGSEPGWVD
                     MKALRDQALRDAASVSLGNLAFREAVAALAAPLHGKRKPDLLDEDQDNARRSRRTRRI
                     VTAALGSLIASLGVAFWTIKGDKKTLDIELQNRTLAERDREIAGIQSAVRKAEQLMST
                     VGHEWEGRALLITERARLIQLAASQSPASASEVETWKLVDGAVLVANHGVQVQLHAAS
                     GARFGSAKLIAGDRFLAVLERFGDDASVHVIDVSDGRDLLDVDGVIGSIAAPSPMARG
                     DQQYILALDDGRIVTRDARGGLNVAAALRNVTSGGESPQRWLSEDGRRLFVARATRTG
                     AEFELYDLPGGTLLERGRLPEEDVPARVIIGAELPSPHVVFPGFPYVYTWEPASRKWQ
                     SIGPFPGMRGIANSVDGRSLYIVGENFPPRLVEANRATTELPDQMYGVQRVMTFPDDA
                     HYLLTIGGRIAGFGIASASCALYLWARGEKPRQYRSFLVEDMDCPEPAIAVARQGMFA
                     YGRPGRPIVVRSVQGHIAATLDQVASARDPADALALTLSESGRSIVIQTDHDVLLVDL
                     PPYDNTGDVRGELATMCGRLPHQRIVNVPTRELDLACREH"
     gene            2868..>3227
                     /locus_tag="VF516_45875"
     CDS             2868..>3227
                     /locus_tag="VF516_45875"
                     /inference="COORDINATES: protein motif:HMM:NF025078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8115166.1"
                     /translation="MPPLLATHASARLGQDFSSVSVHEDGSPERLGTSAYARGDALYF
                     ASGQYRPHTAEGVAKIGHELGHVVQQRGGRVQPTGVRGNVPVNTDPALEREADASGTA
                     VAQGLDIHALFGFGAHRG"
BASE COUNT          533 a         1048 c         1084 g          562 t
ORIGIN      
        1 gttcgccgtc gtcgtcggcg accgcgagcg cagcgagcgc accttgtcgg tccgcgcccg
       61 cgacggtcgg tggagcgccc cgatcgacga ggccgtggct gacctggtgc gccggtgtgt
      121 tgtgccgggg ccgctcgctg cggcatcgtg agccagcacc gcgcgcgatt ccgcggaatc
      181 gcgggggaga ccgttgccgg ctggggagcc gtcgcgtgcg cctcttccgc cgaagatgtg
      241 gcatcatcga cggcggggat aagcgttcgt gcgcatgcga tcgcctcgaa gggcagaatg
      301 atgacatcac ctcgggacac gacgcggagg tatcgagcat tcctttcata cagccatgct
      361 gccgatgatc gctttgcaga ggcattacag aaggctctca agcagttcgc gcgtcccctg
      421 ttcgcggttc gcgcattgaa tatcttcaga gatcgaaccg atctttcggc gacgcccgag
      481 ctctggaaca gcgtgcgctc ggcactcgac gactccgatt acctgatcta tctcgcctcc
      541 cccaaggcgg ccggatctcg gtgggtcaag cgtgaactcg atcactggat ccggacgcgc
      601 tcgacgcttg cgacgctctt gatcgttttg acggatggtg aattgaaatg ggaaggtgcg
      661 gacttcgatt ggtcgcagac gacctcattg cccaggctct tggatttccc cgagctcgca
      721 ggtgaagctc ccctctcgct cgaacagcgc ttcggcagcg agccgggctg ggtcgacatg
      781 aaagctctgc gcgaccaggc gttgcgcgat gccgcgagtg tttcgctcgg caacctggcc
      841 ttcagggagg cggtcgccgc cctcgcggcg ccgctgcacg gaaaacgcaa gccggatctc
      901 ctcgacgagg atcaggacaa cgctcggcgg agccggcgaa cccgtcgcat cgtcaccgcc
      961 gcgctcgggt cgctcatcgc gtctctgggg gttgcattct ggacgatcaa gggtgacaag
     1021 aagacgctcg atatcgagct gcagaaccgc acgctcgccg agcgcgatcg cgagatcgct
     1081 ggcatccagt ccgccgtccg gaaagccgag cagctgatgt cgaccgtggg ccacgaatgg
     1141 gagggccgcg cgttactcat caccgagcga gcacgtctga tccagctggc cgcgtcccag
     1201 agccccgcat ccgcgtccga ggtcgagacc tggaagctcg tcgatggcgc agtgctggtc
     1261 gccaatcacg gcgtacaggt gcagctgcac gcagcgtcgg gagcccgctt tgggagcgca
     1321 aagctgatcg cgggggaccg ttttctcgcg gtgctcgaac gcttcggcga tgacgcctcg
     1381 gtgcacgtca tcgacgtctc cgacggccgt gatctcctgg atgtcgatgg ggtgatcggt
     1441 tcgatcgctg cgccctcccc gatggcgcga ggcgaccagc agtacatcct cgcactcgac
     1501 gacggacgga tcgtgacccg cgacgctcga ggaggcctca acgttgcagc ggcgttgcgc
     1561 aatgtcacca gtggcgggga atcgccccaa cgttggttga gcgaggatgg cagacggctg
     1621 ttcgttgcca gggccaccag gaccggcgcg gagttcgaac tctacgatct gcccggcgga
     1681 accctgctcg aacgtggtcg tctcccggaa gaagacgttc ctgcccgagt gatcatcggg
     1741 gcggaattgc catcgcctca tgtcgttttc ccgggattcc cttacgtata cacatgggag
     1801 cctgcatccc ggaagtggca aagcatcggc ccattcccgg ggatgcgagg aatcgcgaat
     1861 tcggtcgatg gccgatccct ctacatcgtg ggagagaact tcccgccccg actcgtcgag
     1921 gcgaaccgcg cgacgaccga gctgccagac cagatgtacg gggtccagcg agtgatgacg
     1981 ttccccgatg atgctcatta tctcctgacc atcggcggac ggatcgctgg attcgggatc
     2041 gcgagcgcaa gctgtgcgct gtacctgtgg gcgcgaggcg agaagccacg gcagtaccgc
     2101 tctttcctgg tcgaggatat ggactgtccc gaacccgcga tcgccgtggc gcgccagggg
     2161 atgttcgcat acgggcgccc cggaaggcct atcgtggtcc gttccgtaca gggacatatc
     2221 gcggcaacgc tcgatcaggt cgcttcagcc cgtgaccctg ccgatgccct cgcgctcacg
     2281 ttatcagaat ccggccggtc cattgtgatc cagaccgatc acgacgtcct gctcgtggac
     2341 ctgcccccgt acgacaatac cggcgatgtg cgtggcgagc ttgcgacgat gtgcggtcga
     2401 ctcccgcacc agcgcatcgt caatgttccc acaagagagc tcgatctcgc gtgccgggag
     2461 cattgaatgt gtcggggcgc cagctcgcat ggcggatccg agcgagccgg tcgaccttgc
     2521 gctgagctgt gcatgtatcg atcatctttc aaggacacgg tgggctcacc ggtctgctcg
     2581 cccggcaggg gactgcgtat cggcgtcgcg gcgtgaacag gctgctgggg cggcgcgccg
     2641 aatcggcgtg cagcgccgac gccgcgcgtc ctgccgaggc cggcgggacc cggccgcccg
     2701 gtttctcacg cagttgcgca ggcttctggg ccggtatgtc gggtgcaacc agacatgcca
     2761 tgtacgacac gaagcgccgc tttgacagct ccacctcgtc tggcttcgaa cccacgggcg
     2821 ggtctcgcac ccgcggcgac ccgggcaagc agacgctcac ggcggggttg cctccgttgc
     2881 tggcgaccca tgcgagcgcc cggctcggcc aggacttctc gtcggtgagc gtgcacgagg
     2941 atggctcgcc agaacggctg gggaccagcg cgtacgcgcg cggcgacgcg ctgtacttcg
     3001 cgtcggggca gtaccggccc cacaccgccg agggtgtggc gaagatcggc cacgagctgg
     3061 gccacgtcgt gcagcagcgc ggcggccggg tgcagcccac gggcgtccgc ggcaatgtgc
     3121 cggtcaacac ggatccggcg ctcgagcgcg aagccgacgc ctccggaacc gcggtcgcgc
     3181 agggcctcga catccacgcg ctgttcgggt tcggcgcgca tcgcggc
//
LOCUS       DASVOR010002495         4829 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_406642,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002495 DASVOR010000000
VERSION     DASVOR010002495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406642"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..2772
                     /locus_tag="VF516_45880"
     CDS             268..2772
                     /locus_tag="VF516_45880"
                     /inference="COORDINATES: protein motif:HMM:NF039722.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6311 domain-containing protein"
                     /protein_id="HEX8115167.1"
                     /translation="MRHRRLLIWLLAIAVGSTVFFVWMGYARTLDPRDLGWIFHEDPF
                     AHEMGWEQYRNAPLLQYPITKNTLYGLEWGSTIVFTDSIPIVALVLRPLSDLLPRPCQ
                     YDGWWVLLSLVLQAYWAARLVLLRSDRLGDAALGCIFFVTAPVLVERLGLQTGVGSHW
                     LLLWALWLYFGSRGPATRSWAVLLLLTVSVHAYLFVMVSAIWIAHLVSQRLQGQLPPR
                     AIVLAGGVVIAVIAWMHALGYFMVGDGAAGGTWRSSFDLLGFVSPCAGAWFDLVPVMY
                     NDPWDGTSYLGAGALALLIASTAARVATRRSPALSGGGPRVPWAPLLAAVVGLGAFAL
                     TNHITFAHKLIGRYPLPPGFSGLYDTFRGAARMIWPAYYLVLLATLWLALRAWPQRWR
                     TLVIALAAALQLVDLTGVGAAKRGQIQGDGLIRPLRDPIWAVIAQHYRSIISVPAYHR
                     QADWPTFAWFAARNRLGSDIAYSSRNNPAVRQAGTRAHLDAVASGHYDPQTVYYFPSS
                     DLWTVARGTMGPQDLAVVADGYHLVLPGGRAWVAAAPPAAPPGVPRLGTWIPFSPSDA
                     TGLLVDGWSWPEKWGTWSIEPLPSLVLPVPPNERVRVSFQWQVAGLPQQARTMCVFLD
                     DEMFPVRFLRRYFRQKNSFEVTTRGPLLTVRLGFTDLTPHNPNSRAAEVGILAARVER
                     ADQLSADEVPAAATAPAVLDDWISFAAHAPGRAFLGDGWSWGEVWGTWSDYPAPELAL
                     PVPPGERLAVSFRWLATAPQHQVQSVRVVIDDRPFLIQFPTALQDQESTFEVTSRRPW
                     IAVRLEIDRPVLSRGRELGIGLKAARVHRIAGPGRE"
     gene            complement(2838..3464)
                     /locus_tag="VF516_45885"
     CDS             complement(2838..3464)
                     /locus_tag="VF516_45885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115168.1"
                     /translation="MTKGHTVITGTTRTFGRDGALIATLPSPRVTLDAHGDVATEERL
                     SATGVVELRRQFTGGRLVAEERFAADGQLDERVAYRYDASGHLAEEVMVFGDGTPHGT
                     WIHRRDPSGRLIGRAFVKPDGSTEATETYRHAADGRSATMVRAHVGQWTYRYDDDGRI
                     LHEEGGPASGDDMDRVAIDYTYDARHRLTSEITRGPGGRVQRELTIAY"
     gene            complement(3568..4782)
                     /locus_tag="VF516_45890"
     CDS             complement(3568..4782)
                     /locus_tag="VF516_45890"
                     /inference="COORDINATES: protein motif:HMM:NF039793.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HEX8115169.1"
                     /translation="MTTVEVRPSGLPTPMVDLDPVDVSGIQAHVGGAAAEGARAMGAQ
                     AYATGDHVAFAEAPDLHTAAHEAAHVVQQRAGVQLKGGVGETGDAYERHADAVADCVV
                     QGKSAESLLDTMAGGGGAAAVQRKIVVGGKDYDPKPDLPEVKKKYGNDMVEALTTMHN
                     GGKAPPEFKYATKEELMTELSLRDNATKGMGKANASGANLRYAGSGDDPEGHLDPKFW
                     DKKGFYQFTLKPGAKPADAVRSIFDNKDNVLECNSTMVAIEYRSMLDTMGDAKFNARF
                     AGGGLIISPHHVPMPDGSAHPLHTEGMIDEVTITGAKDLIPGDWVYFKNFPDYGAKHP
                     GGFWTGEHTMYLGDGKFQGFGTTVSTEAEINQKLLDNYNSGLPAAQQKKLADVPGLQN
                     YARRPVANKMVK"
BASE COUNT          598 a         1711 c         1685 g          835 t
ORIGIN      
        1 ggatgcgccg gcgatggtag agggcacgtc gaccgaatcc ggggctcggg gcgccgctcc
       61 accgcgccgg ccgtgcgcgc cgagccggtg gtgcgacgat ccgggccatt cgcacatcga
      121 tcggcacggt ccggcagccg ccacgaccgt gcgcacgacg ctggacgcga tcgtgagcag
      181 ccactacact ccgcgccgtc catgggtgtt cgcgagcggc cgacgacgct gaccacacca
      241 cagcccggga cggatccgat cgcgcgcatg cggcatcggc gactcctgat ctggctgctc
      301 gcgatcgcgg tgggcagcac ggtgttcttc gtctggatgg ggtacgcgcg aacgctggat
      361 ccccgggacc tcggatggat cttccacgag gatccgttcg ctcacgagat gggctgggag
      421 caataccgca acgcgccgct gctgcaatac ccgatcacga agaacacgct ctacgggctc
      481 gagtggggca gcacgatcgt gttcacggac tcgatcccga tcgtcgcgct cgtgctgcgc
      541 ccgctgagcg acctcttgcc tcggccctgt caatacgacg gctggtgggt gctgctgtcc
      601 ctggtgctgc aagcgtactg ggcggctcgc ctggtgctcc tgcgctccga tcggctgggc
      661 gacgccgcgc tcggttgcat cttcttcgtg acggcccccg tcctcgtgga gcggctcggt
      721 ctgcagaccg gcgtcggcag ccactggctc ctgctgtggg cgctctggct gtacttcgga
      781 tcgcgcggcc cggcgacgcg atcgtgggcg gtgctgctgc tgctcacggt cagcgtgcat
      841 gcctacctgt tcgtcatggt cagtgcgatc tggatcgccc acctggtcag ccagcgcctg
      901 caaggacagc tgccgccgcg cgcgatcgtg ctcgcaggtg gggtggtgat cgccgtgatc
      961 gcctggatgc acgccctggg gtacttcatg gtcggcgacg gcgccgccgg cggcacctgg
     1021 cgctccagct tcgacctcct cggtttcgtg tcgccgtgcg cgggagcgtg gttcgacctg
     1081 gtgcccgtca tgtacaacga cccgtgggac ggcacctcgt acctcggcgc gggcgccctc
     1141 gccctgctga tcgcgagcac cgccgcccgg gtcgcgacgc ggcgatctcc ggcgctgagc
     1201 ggcggcggcc ctcgggtgcc ctgggcgccg ctgctcgcgg ccgtggtcgg cctgggcgcg
     1261 ttcgcgctca ccaaccacat caccttcgcg cacaagctga tcgggcgcta tccgctgccc
     1321 ccggggttct ccggcctgta cgacaccttc cgcggcgccg cgcggatgat ctggcccgcg
     1381 tactacctgg tcctgctggc gacgctgtgg ctggccctgc gcgcctggcc gcagcgatgg
     1441 cgcaccctgg tgatcgcgct ggccgcggcg ctgcagctcg tcgatctcac cggcgtcggc
     1501 gcggccaagc gagggcagat ccagggcgac gggctcatcc gcccgctccg cgatccgatc
     1561 tgggcggtga tcgcgcagca ctaccgctcg atcatcagcg tgcctgccta tcatcgccag
     1621 gccgactggc cgaccttcgc ctggttcgcc gcccgcaacc gcctcggctc cgacatcgcc
     1681 tactcgagcc ggaacaaccc cgccgtgcgg caggccggca cgcgcgcgca cctggacgcc
     1741 gtggcgagcg gccactatga cccgcagacc gtctactact ttccgagctc ggacctctgg
     1801 acggtggcgc gcgggaccat gggtccgcag gacctcgcgg tggtggccga cggctatcac
     1861 ctggtgctgc ccggtgggcg cgcatgggtc gccgcggcac cgcccgccgc gccgcccggc
     1921 gtgcccaggc tgggaacgtg gatcccgttc tcgccgtccg acgcgaccgg attgctggtc
     1981 gacggctggt catggccgga gaagtggggg acctggtcca tcgaaccgct gccgtcgctc
     2041 gtgctgccgg tccctcccaa cgagcgcgtg cgcgtgtcgt tccagtggca ggtcgccggc
     2101 ctaccgcagc aagcgcgcac gatgtgcgtg ttcctggacg acgagatgtt cccggtccgc
     2161 ttcctgcgcc ggtacttccg gcagaagaat agcttcgagg tgacgacccg gggcccactg
     2221 ctcaccgtgc gcctcgggtt caccgatctg acaccgcaca acccgaactc ccgggcggcc
     2281 gaggtcggca tcctcgcggc gcgcgtcgag cgcgctgacc agctctcggc cgacgaggtg
     2341 cccgcggccg ccaccgcgcc tgccgtgctc gacgactgga tctcgttcgc cgcccacgcg
     2401 ccgggccgcg ccttcctggg agacggctgg tcgtgggggg aggtgtgggg gacgtggtcc
     2461 gattatccgg ctccggagct cgcgctgccg gtgccacccg gagagcgcct cgccgtctcg
     2521 ttccgctggc tcgccaccgc gccgcagcac caggtgcagt cggtccgcgt ggtcatcgac
     2581 gaccggccgt tcctcatcca gttccccacc gccctgcagg accaggagag cacgttcgag
     2641 gtcacgagcc ggcggccgtg gatcgcggtc cggctcgaga tcgaccggcc ggtcctgtcg
     2701 cgtggaagag agctcgggat cgggctcaag gccgcccgcg ttcaccgcat cgccggaccc
     2761 ggtcgcgagt gacatcgcac ggcgacgggg ccacgaccgg atcgaagtgc gcggtgatca
     2821 gcggtgttgc ggtgcgctca gtacgcgatg gtcagctcgc gctgcacgcg gccgccgggc
     2881 ccacgcgtga tctcgctggt cagccggtgg cgggcatcgt acgtgtagtc gatcgcgacg
     2941 cgatccatgt cgtcgccgga ggccgggccg ccttcctcgt gcaggatccg gccgtcgtcg
     3001 tcgtagcggt acgtccactg gccgacatga gcgcgcacca tcgtcgccga tctgccgtcg
     3061 gcggcgtggc ggtaggtctc ggtggcctcg gtgctgccgt cgggcttgac gaaggcgcgg
     3121 ccgatgagcc ggccgctcgg gtcgcggcgg tggatccatg tcccgtgcgg cgtgccatcg
     3181 ccgaacacca tcacttcctc ggcgaggtgg ccgctggcgt cgtagcggta cgccacgcgc
     3241 tcgtcgagct ggccgtcggc ggcgaaccgc tcctcggcaa cgagccggcc gccggtgaac
     3301 tgccggcgca gctcgacgac gccggtggcc gacagccgct cctcggtcgc gacgtcgccg
     3361 tgcgcgtcga gcgtgacgcg cggcgacggc agcgtcgcga tgagcgcgcc gtcgcgcccg
     3421 aacgtcctgg tcgtgccggt gatcacggtg tgtcccttgg tcatgttcga aatccccgtc
     3481 ggctcgcgcg tgtccggagg ctgactcgcg cgggggcagc ctgcgagcgc gaccccgatc
     3541 gcgatgtgcg ccatgcttcg catgccgcta tttgaccatc ttgttggcga ccgggcgacg
     3601 cgcgtagttc tgcaggccgg gcacgtctgc gagcttcttc tgctgcgccg ccggcagccc
     3661 gctgttgtag ttgtccagca gcttctggtt gatctcggcc tcggtcgaca cggtcgtgcc
     3721 gaagccctgg aacttgccat cgccgaggta catcgtgtgc tcgccggtcc agaacccacc
     3781 gggatgcttg gcgccgtagt ccgggaagtt cttgaagtac acccagtcgc cggggatgag
     3841 gtccttcgcg ccggtgatcg tcacctcgtc gatcatgccc tcggtgtgca gcggatgggc
     3901 gctgccgtcg ggcatcggca cgtggtgcgg cgagatgatc aggccgccgc ccgcgaaccg
     3961 cgcattgaac ttggcgtcgc ccatcgtgtc gagcatcgaa cggtattcga tcgcgaccat
     4021 cgtcgagttg cactcgagga cgttgtcctt gttatcgaag atcgagcgca cggcgtcggc
     4081 cggcttggcg ccgggcttga gcgtgaactg gtagaagcct ttcttgtccc agaacttcgg
     4141 gtcgaggtgg ccttcggggt cgtcgcccga ccccgcgtaa cgcaggttcg cgccgctggc
     4201 gttcgccttg cccatcccct tggtggcgtt gtcgcgaagg ctgagctcgg tcatgagctc
     4261 ctccttcgtg gcgtacttga actcgggcgg cgccttgccg ccgttgtgca tcgtggtcaa
     4321 cgcctcgacc atgtcgttgc cgtacttctt cttgacctcg ggcaggtcgg gcttggggtc
     4381 gtagtccttg ccaccgacga cgatcttgcg ctgcaccgcg gcagctccgc caccgccggc
     4441 catcgtgtcg agcagcgact cggcggactt gccctgcacc acgcagtcgg cgacggcatc
     4501 ggcgtggcgc tcgtaggcgt caccggtctc accgacgccg cccttgagct gcacccccgc
     4561 gcgttgctgg acgacgtggg cggcttcgtg cgcggccgtg tgcaggtcgg gcgcctcggc
     4621 gaacgccacg tgatccccgg tggcataggc ctgtgcgccc atcgcgcgcg cgccttcggc
     4681 cgcggcgccg ccgacgtggg cctggatgcc gctgacgtcg accgggtcca gatcgaccat
     4741 cggtgtcggg aggccggagg gccggacctc caccgtcgtc accggatcct cgccacgctc
     4801 ggtctatgga acaatcgcgg cgcaacggg
//
LOCUS       DASVOR010002496         2202 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_232332,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002496 DASVOR010000000
VERSION     DASVOR010002496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232332"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="VF516_45895"
     CDS             complement(<1..1225)
                     /locus_tag="VF516_45895"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8115170.1"
                     /translation="MLDDKYRVERLLAAGGMGEVYLGTHIGLHKRVAIKVINPQTSTP
                     AMVERFHREAITASQIGHEGIAQVTDIGTSGDGEPFLVMEYLEGESLASRLTVSAPLA
                     IENACELGCAILSPLAAAHRAGIVHRDLKPDNVFLVRQSRGEMVKLLDFGISRFTGLE
                     RDFRLTATGLVLGTPYYMSPEQARGDSMVSTASDLYAFGVILYEMLVGDVPIRAENYN
                     ALMYRVTIGDFQRPRERRSDIPRPLEHIILCAMAQAPEDRPRARELETTLLPFCRQAF
                     RDGSGRITALGPSHRTPAPADADDSLRTLDPVTFSGVTEPLQAVEPPAEEKTPGGLEP
                     GPPSDGELVVPAAPPDLQGPTIAVPRSRALRVVVACSIVTAGALAAVVITRRITDDGP
                     RTATAAEFPRPGPATA"
     gene            complement(1577..>2202)
                     /locus_tag="VF516_45900"
     CDS             complement(1577..>2202)
                     /locus_tag="VF516_45900"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEX8115171.1"
                     /translation="VDRRDRPLEPQLGAYGELRMTEGTRLAGGAFDYFEAIGDVRGFA
                     PLLAGVVLAARARYGGIWGDIPVTERLFSGGATSQRGFGERRLAPFVHGTTFHPDGTV
                     SGFAYIPYGGGGLFETSVEARVPVTTIRGMRFGIVTFLDGGDVTETPGELDLGDLNWA
                     VGGGLRLITAIGPVRFDIGYRLNRTSGPNDPDPGQHFAYHLTIGEAF"
BASE COUNT          294 a          794 c          813 g          301 t
ORIGIN      
        1 gtgcggtcgc ggggccgggc cgcgggaact cggccgccgt tgcggtccgt gggccgtcgt
       61 cggtgatccg gcgcgtgatc accacggcgg cgagcgcccc ggcggtgacg atcgagcagg
      121 cgacgaccac ccgcagcgcg cgcgatcgcg gcaccgcgat cgtcgggccc tggaggtcgg
      181 gcggcgcggc cggcacgacg agctcgccgt ccgagggcgg gcccggctcg agtcctccgg
      241 gcgtcttctc ctcggcgggg ggctcgaccg cttgcagcgg ctcggtgacg ccggagaacg
      301 tcaccggatc cagcgtccgt agcgagtcgt cggcgtcggc cggcgcgggg gtccggtgcg
      361 atgggccgag cgcggtgatc cggccgctgc cgtcgcggaa cgcctggcgg cagaacggca
      421 gcagtgtcgt ctccagctca cgcgcgcgcg gccggtcctc gggcgcctgc gccatcgcgc
      481 acaggatgat gtgctccagc ggccggggga tgtcgctgcg gcgctcgcgc ggccgctgga
      541 agtcgcctat cgtgacccgg tacatcagcg cgttgtagtt ctccgcgcgg atcggcacgt
      601 cgccgaccag catctcgtac aggatcacgc cgaacgcgta gagatccgac gccgtggaca
      661 ccatgctgtc gccgcgcgcc tgttcgggcg acatgtagta cggcgtgccg agcacgaggc
      721 cggtcgcggt cagccggaag tcgcgctcca ggccggtgaa ccgcgagatg ccgaagtcga
      781 gcagcttgac catctcgccg cggctctggc ggaccaggaa cacgttgtcg ggcttgagat
      841 cgcggtggac gatgccggcg cgatgggcgg cggcgagcgg cgacaggatc gcgcagccga
      901 gctcgcaggc gttctcgatc gccagcgggg cgctcaccgt gagccggctc gccagcgact
      961 cgccctccag gtactccatc accaggaacg gctcaccgtc gccgctggtc ccgatgtcgg
     1021 tgacctgggc gatgccctcg tggccgatct gcgaggcggt gatcgcctcg cggtggaacc
     1081 gctcgaccat cgcgggggtg ctggtctgcg ggttgatcac cttgatcgcc acccgcttgt
     1141 gcagcccgat gtgcgtgccg aggtagacct cgcccatccc accggccgcg agcaagcgct
     1201 cgacgcggta cttgtcgtcg agcatggtgc cctcggacag ctggcggggt cccgcagcgc
     1261 cttccactcc gaccgagaat aagccacgat gcgcgtgaat tcaccccgaa ccacggccgg
     1321 cgagtcgggt ccgggaggcc ggtcgtgaga tcgccgggcg ccgccgctga cggtcgctgc
     1381 gggccggcgc gccgcggcgc tccatcgcgg cccaaagcgg attcagtggc gccctgcgtt
     1441 gcgtcgcgcg gggcgcgtac gcgatcgagc ccgccgggtg cgagagggag ctcccagctc
     1501 tcaccccgcg gcggtgcctg gaacctgcgc gcggcgcagc gcctgctcgc caccgcgctc
     1561 ggagtcccgg tcgcgatcag aaggcctccc cgatcgtgag gtggtacgcg aagtgctgtc
     1621 cgggatccgg atcgttcggc cccgacgtgc ggttcagccg gtagccgatg tcgaaccgga
     1681 ccgggccgat cgccgtgatc agccgcaggc cgccgcccac cgcccagttc aggtccccca
     1741 ggtcgagctc gcccggcgtc tcggtgacgt cgccaccgtc gaggaacgtg acgatgccga
     1801 accgcatgcc gcggatcgtg gtgaccggca cgcgggcctc gacgctggtc tcgaacagcc
     1861 caccgccgcc gtacgggatg tacgcaaagc cgctgaccgt gccgtccggg tggaacgtcg
     1921 tgccgtggac gaacggcgcg agccggcgct cgccgaagcc gcgctggctg gtcgcgccgc
     1981 ccgagaacag ccgctcggtc accgggatgt cgccccagat cccgccgtag cgggcgcgcg
     2041 ccgcgagcac gaccccggcg agcagcggcg cgaagccgcg cacgtcgccg atcgcctcga
     2101 agtaatcgaa cgcgccgccc gccaggcgcg tgccctcggt catgcgcagc tcgccgtacg
     2161 ccccgagctg cggctcgagc ggcctgtcgc gccggtcgac ga
//
LOCUS       DASVOR010002497         2091 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_554548,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002497 DASVOR010000000
VERSION     DASVOR010002497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_554548"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VF516_45905"
     CDS             complement(<1..597)
                     /locus_tag="VF516_45905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115172.1"
                     /translation="MLAVALVAAVIAGDHVVLRASPSRTAAQQAVLWRGDWLEVRGEQ
                     RGWLKVYDHRHERPGWVARPSARTVELDDHAAPALRAVVEFLRDAPGSESLGIAYAGL
                     YMKVAPRGGIDASLLVSLGTMADRLAHRASSEAADGHAAQQLEVAQSWGIGFTTVERL
                     DGIRTCYDGAAFRQALALAPSGNDLATAVLGLTDPACEP"
     gene            complement(597..1844)
                     /locus_tag="VF516_45910"
     CDS             complement(597..1844)
                     /locus_tag="VF516_45910"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEX8115173.1"
                     /translation="MRTVLSKLQTGLSRIGYLFKATALACVLGVIEMVQTQRGRRVLG
                     ALTLIALVVGTLVTRPIRSVPPGEAAVRVNRLTGGVAILDEGWIVVVPGLHEFRRYPL
                     HDQVYRPADAAHAGGTGAFQSVEGLSVGVDVSVRWALDPSRMSQAARLRPEEIGAELV
                     VPTVDGALHGAFAQHTVREIFATQRAPIQKALEDDLRQTLAKDGVIVKAVVIGSIDLP
                     EKYKAGLEALLSEELAAEKMRYTLELKEKSIKQTELEAEADKIRRQKAAEAAGAEQVI
                     AARSQEEAMAHVLPLKEKEIDQKRLEAEAAKVSRLKAAEANAEARRIEAGGEADSRRK
                     LADSDAYRLEVTGKASTEQMAREAALLAKNPLLIQKTLADKLSDKISVIIAPPNAGGF
                     IAGNLLGGGGAAATQPAKLDEDR"
BASE COUNT          254 a          759 c          769 g          309 t
ORIGIN      
        1 gggctcgcac gccggatcgg tcagcccgag cacggcggtg gcgaggtcgt tgcccgacgg
       61 tgcaagcgcg agcgcctggc ggaacgccgc gccgtcgtag caggtccgga tcccgtcgag
      121 gcgctcgacg gtggtgaacc cgatgcccca gctctgcgcg acctcgagct gctgcgcggc
      181 gtggccgtcg gcggcctcgc tcgatgcccg gtgggcgagc cggtcggcca tcgtgccgag
      241 cgagaccagg agcgatgcgt cgatcccgcc gcgcggcgcg accttcatgt acaggccggc
      301 gtaggcgatg ccgagcgact ccgagcccgg cgcgtcgcgc aggaactcga cgacggcgcg
      361 cagcgcgggc gccgcgtggt cgtcgagctc gacggtgcgc gccgacggcc gggcgaccca
      421 gcccgggcgc tcgtggcggt ggtcgtagac cttgagccag ccgcgctgct cgccgcggac
      481 ctcgagccag tcgccgcgcc acagcaccgc ctgctgggcc gcggtgcggc tcggcgacgc
      541 gcgcagcacg acgtggtcgc cggcgatcac cgcggcgacg agcgcgaccg ccagcatcag
      601 cgatcctcgt cgagcttggc cggctgggtc gccgccgcgc cgccgccgcc gagcaggttg
      661 ccggcgatga agccacccgc gttgggcggc gcgatgatca ccgagatctt gtcgctcagc
      721 ttgtcggcga gcgtcttctg gatcagcagc gggttcttgg cgagcagcgc ggcctcgcgc
      781 gccatctgct cggtcgacgc cttgccggtg acctcgagcc ggtaggcgtc gctgtcggcg
      841 agcttgcggc gcgagtcggc ctcgccgccg gcctcgatcc ggcgagcctc ggcgttggcc
      901 tcggccgcct tgagccggct gaccttggcg gcctcggcct cgagccgctt ctggtcgatc
      961 tccttctcct tgagcggcag cacgtgggcc atcgcctcct cctgcgacct ggcggcgatc
     1021 acctgctcgg cgccggcggc ctcggctgcc ttctggcggc ggatcttgtc ggcctcggcc
     1081 tcgagctcgg tctgcttgat cgatttttcc ttgagctcga gcgtgtagcg catcttctcg
     1141 gcggcgagct cctcggagag cagcgcctcg agccccgcct tgtacttctc cggcaggtcg
     1201 atgctgccga tcaccaccgc cttgacgatc acgccgtcct tggcgagggt ctggcgcagg
     1261 tcgtcctcga gcgccttctg gatcggggcg cgctgggtcg cgaagatctc gcgcacggtg
     1321 tgctgcgcga acgcgccgtg cagggcgccg tcgacggtcg gcacgaccag ctcggctccg
     1381 atctcctcgg gccggagccg cgcggcctgc gacatccgcg agggatcgag cgcccaccgc
     1441 acgctgacgt cgacgccgac cgacaggccc tcgaccgact ggaacgcgcc ggtgccgccg
     1501 gcgtgcgcgg cgtcggccgg ccggtacacc tggtcgtgca gcgggtagcg ccggaactcg
     1561 tggaggccgg gcacgacgac gatccagccc tcgtccagga tggcgacgcc gccggtcagc
     1621 cggttgaccc ggacggcggc ctcgccgggc ggcaccgacc ggatcggccg ggtcaccagg
     1681 gtgccgacca ccagcgcgat cagggtcagc gcgccgagca cgcgccggcc gcgctgggtc
     1741 tggaccatct cgatcacgcc gaggacgcag gccagcgcgg tcgccttgaa caggtagccg
     1801 attcgtgaaa gtccggtctg gagcttcgat aggacggttc gcatgggttc acctgcgcgg
     1861 ccgcgactgc acggcgcgat ccaggcaccg gctccgtcga cgatctgcgg caagtgctca
     1921 ccgggacacg aggcgcgtgt ccggcgagcc acgcgatcgg cggcgatcgc gccgcggcga
     1981 atacggcgac cggcgcgcgc gttgtctggt aggttgccgc gatgcaggtc gtcgcgcatg
     2041 ccgtccgctc gggcatcgcg gcgctcaacg tcgtgaccgc cgcgctcgac a
//
LOCUS       DASVOR010002498         1944 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_86441,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002498 DASVOR010000000
VERSION     DASVOR010002498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86441"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VF516_45915"
     CDS             complement(<1..642)
                     /locus_tag="VF516_45915"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEX8115174.1"
                     /translation="MTRRVEADAEAVAATTAALAAAPLVAFDLEFLAQDRLVPTLCLV
                     QVAWLEHVRLDAPAAAIVAAPPQVRLIDPLAVDVAPLVRALAAHPCAVAHAPRQDLAL
                     LAARFGVAMPAVVDTQVMAAFAGIGDQVGFAALANELLGLTLGKELQWTDWAARPLSD
                     AQLAYADADVRHLPAIYARLADRLGDRLAWARAESALVAADAFAAAQVTPETAW"
     gene            688..>1944
                     /locus_tag="VF516_45920"
     CDS             688..>1944
                     /locus_tag="VF516_45920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012831741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEX8115175.1"
                     /translation="MTKTNSFSSKAQIAVGRGKVELYRLDALVKAGVGDVATLPYSLR
                     ILLENLLRHEDGRTVHPADIQAVAGWNPRQRVAHEVQLRPARVLMQDFTGVPAVCDLA
                     AMRDAFVQLGLPGKAINPLKPVDLVIDHSVQIDEYGTHAAFRRNVELEYQRNHERYLF
                     LRWGQQAFANMKVVPPGTGICHQVNLEYLARVVFTESGSHTGGHVLAYPDSLVGTDSH
                     TTMVNGLGVFGWGVGGIEAEAVMLGQPMAMLIPEVVGFELTGQLPAGATATDLVLTVT
                     QMLRKKGVVDKFVEFTGPGVAALSLPDRATIANMAPEYGATMGFFPVDAETLAYLRFT
                     GRSDDHVELVERYAKENLLWHDPGARLRFVDTLALDLSTVEPSLAGPARPQDRVPLKT
                     SRRAWRRAVGSILGDRSGTDAAAVDAW"
BASE COUNT          285 a          733 c          676 g          250 t
ORIGIN      
        1 ccacgcggtc tcgggggtca cctgggccgc ggcgaacgcg tcggccgcca ccagcgcact
       61 ctcggcgcgc gcccacgcca gccggtcgcc gagccggtcg gcgagccgcg cgtagatcgc
      121 cggcaggtgg cggacgtcgg cgtcggcata ggcgagctgc gcatcggaca gcggccgcgc
      181 cgcccagtcg gtccactgca gctccttgcc gagcgtgagc ccgagcagct cgttggcgag
      241 cgccgcgaac ccgacctgat cgccgatccc ggcgaacgcg gccatgacct gggtatcgac
      301 gaccgccggc atcgcgacgc cgaaccgcgc ggcgagcagc gcgaggtcct ggcgcggcgc
      361 atgggcgacc gcgcacgggt gggcggccag ggcacggacc agcggcgcga cgtcgacggc
      421 gagcggatcg atcagcctca cctggggcgg cgccgcgacg atcgccgccg ccggtgcatc
      481 caagcgtacg tgctccagcc aggcgacctg aaccaggcac agcgtcggca ccagccggtc
      541 ctgcgccagg aactcgaggt cgaacgcgac cagcggcgcg gcggccagcg cggcggtggt
      601 cgccgcgacg gcctccgcat cggcctccac gcgcctggtc acggggcgcg atactcgccc
      661 acatcctgct agattggcaa agcccccatg acgaagacga acagtttttc cagcaaggcg
      721 cagatcgcgg tcggtcgcgg caaggtcgag ctctaccggc tcgacgccct cgtcaaggcc
      781 ggggtcggcg acgtcgcgac cctgccctac tcgctgcgca tcctgctcga gaacctgctg
      841 cgccacgagg acggcaggac cgttcacccc gccgatatcc aggcggtcgc cggctggaat
      901 cccaggcagc gcgtcgcgca cgaggtccag ctccggccgg cgcgcgtgct gatgcaggac
      961 ttcaccggcg tgccggcggt gtgcgacctc gccgcgatgc gcgacgcgtt cgtccagctc
     1021 gggctgcccg gcaaggcgat caacccgctc aagccggtcg acctcgtgat cgaccactcg
     1081 gtgcagatcg acgagtacgg cacccacgcg gcgttccgcc gcaacgtcga gctcgagtac
     1141 cagcgcaacc acgagcgcta cctgttcctg cgctggggcc agcaggcgtt cgccaacatg
     1201 aaggtcgtgc cgcccggcac cggcatctgc caccaggtca acctcgagta cctcgccagg
     1261 gtcgtgttca cggagtccgg gtcgcacacc ggcggccacg tcctggcgta cccggactcg
     1321 ctggtcggca ccgactcgca caccaccatg gtcaacggcc tgggcgtgtt cggctggggc
     1381 gtcggcggca tcgaggccga ggcggtcatg ctcggccagc cgatggcgat gctgatcccc
     1441 gaggtcgtcg gcttcgagct caccggccag ctgccggccg gcgcgaccgc caccgacctg
     1501 gtgctcaccg tgacccagat gctgcgcaag aagggcgtgg tcgacaagtt cgtcgagttc
     1561 accggcccgg gcgtcgcggc gctgtcgctc cccgatcgcg ccacgatcgc caacatggcg
     1621 cccgagtacg gcgcgaccat ggggttcttc ccggtcgacg ccgagaccct ggcctacctg
     1681 cggttcaccg gccgcagcga cgaccacgtc gagctggtcg agcgctacgc caaggagaac
     1741 ctgctgtggc acgacccggg cgccaggctg cggttcgtcg ataccctggc gctcgacctg
     1801 tcgaccgtcg agccgtcgct cgccggcccg gcgcggcccc aggaccgcgt tccgctcaag
     1861 acctcgcgcc gcgcgtggcg ccgcgccgtc ggctcgatcc tcggcgaccg gagcggcacc
     1921 gacgcggccg cggtcgacgc ctgg
//
LOCUS       DASVOR010002499         1505 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_144545,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002499 DASVOR010000000
VERSION     DASVOR010002499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144545"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1505
                     /locus_tag="VF516_45925"
     CDS             <1..>1505
                     /locus_tag="VF516_45925"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEX8115176.1"
                     /translation="QLAWLARLGRGRRLAAWLAMPAVWLGLLGLVLATGGSAAVIKAA
                     SAYTGLGGPISRALRHAFDRDGDGYSRFLGGGDCDDSDPSVHPGAPEIPDDGIDQNCL
                     GGDATAHRAPADPAFHPVPPGVPRDANILLITIDTTRADHLGMYGYSRPTSPNLDAMA
                     RAATVFEHGWAHAPSTRYSMPAILTGRLPLDVHYDTSVDGWPGLLPRATTIAEALAPL
                     GFVTGAITNYWYFDRIRHMDQGFAEYDNEDARLHTGVPGAGPEQTRGSSSKQQTDKAI
                     GFVTRHAAERWFLWVHYYDPHYAYEPHPEVPSFTPSAGGDRVALYDGELRFTDLHIGR
                     LLDELRAQKLYDRTIIVVTGDHGEGFGEHGIELHGYHLYAAQTKVPLIIRVPGLPPRR
                     AATPAGHADILPTLVNLAGGAASPDMMGRSLVDALAGTDAPRTIFQQLSYEGNHEMRA
                     GVDAHCHVIYNASPDTSWEVYRVDRDPMEAHDLADDDECAGTHRAVEQWVD"
BASE COUNT          215 a          604 c          497 g          189 t
ORIGIN      
        1 ccagctcgcg tggctcgccc ggctcgggcg ggggcggcgg ctcgccgcgt ggctcgcgat
       61 gcccgcggtc tggctcgggc tgctcggcct cgtgctcgcg accggcggct cggccgcggt
      121 gatcaaggcg gcctcggcgt acaccggcct cggcggcccg atctctcgcg cgctgcgcca
      181 cgcgttcgac cgcgacggcg acggctactc gcggttcctc ggcggcggcg actgcgacga
      241 cagcgacccc agcgtccacc ccggcgcgcc ggagatcccc gacgacggca tcgatcagaa
      301 ctgcctgggc ggcgacgcca ccgcccaccg cgccccggcc gatccggcgt tccacccggt
      361 gccgcccggc gtgccgcgcg acgccaacat cctgctgatc acgatcgaca ccacgcgcgc
      421 cgatcacctc ggcatgtacg gctactcgcg gccgacctcg cccaacctcg acgcgatggc
      481 gcgcgcggcg accgtgttcg agcacggctg ggcccacgcg ccgtcgaccc gctactcgat
      541 gcccgcgatc ctgaccggcc ggctgccgct cgacgtccac tacgacacct cggtcgacgg
      601 ctggcccggc ctgttgccca gggcgacgac gatcgccgag gcgctcgccc cgctcggctt
      661 cgtcaccggc gcgatcacca actactggta cttcgaccgg atccgccaca tggaccaggg
      721 cttcgccgag tacgacaacg aggacgcccg gctgcacacc ggggtcccgg gcgccggccc
      781 cgagcagacc cgcggcagct cgtcgaagca gcagaccgac aaggcgatcg gcttcgtcac
      841 ccggcacgcc gccgagcgct ggtttttgtg ggtccactac tacgatccgc actacgccta
      901 cgagccgcac cccgaggtgc cgtcgttcac accctcggcc ggcggcgatc gcgtcgcgct
      961 gtacgacggc gagctgcggt tcaccgacct ccacatcggc cggctgctcg acgagctgcg
     1021 cgcgcagaag ctgtacgacc ggacgatcat cgtcgtcacc ggcgaccacg gcgagggctt
     1081 cggcgagcac ggcatcgagc tccacggcta tcacctgtac gcggcgcaga ccaaggtgcc
     1141 gctgatcatc cgggtccccg gcctgccgcc gcgccgcgcc gccacgccgg ccggccacgc
     1201 cgacatcctg ccgaccctgg tcaacctcgc cggcggcgcc gcgagcccgg acatgatggg
     1261 ccgctcgctg gtcgacgccc tcgccggaac cgacgcgccg cgcacgatct tccagcagct
     1321 gtcgtacgag ggcaaccacg agatgcgcgc cggggtcgat gcgcactgcc acgtgatcta
     1381 caacgccagc cccgacacca gctgggaggt ctaccgcgtc gaccgcgatc cgatggaggc
     1441 ccacgacctg gcggacgacg acgagtgcgc cggcacccac cgcgcggtcg agcagtgggt
     1501 cgacg
//
LOCUS       DASVOR010002500         5555 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_289683,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002500 DASVOR010000000
VERSION     DASVOR010002500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289683"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VF516_45930"
     CDS             <1..553
                     /locus_tag="VF516_45930"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8115177.1"
                     /translation="FSYYGFFTWIPSLLVKQGMTTTKSFGYSIIIYLAQIPGYYSAAF
                     LSEKLDRKWTIVVYMVLGGVSAFLMSSAGSGASITAAGFLLSFFMNGTYAGIYAYTPE
                     LYPTAFRTTGMGVASAFGRLGGLSAPIVIGHAYDRIGFGGVFVMTTVVLVAGALAVAL
                     LGIATAGKSLEEITAEALRPADP"
     gene            complement(572..1417)
                     /locus_tag="VF516_45935"
     CDS             complement(572..1417)
                     /locus_tag="VF516_45935"
                     /inference="COORDINATES: protein motif:HMM:NF041278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CmcJ/NvfI family oxidoreductase"
                     /protein_id="HEX8115178.1"
                     /translation="MFAATESSELDEVTAEVNYSVDTGVKPLDESYGPGNVHSRKLGA
                     IEPRRVRIRNARRLAGQLALERNGFVLVEHATRVADLLDKPRIPELYYPEVEALVAQV
                     SGARRVVVFDHTVRSSADLAEREAKQLQEPVLLVHNDYTEWSGPQRVRDVLPAEADAL
                     LRHRFAIIQVWRPIARPAERNPLAIVDARSLAPGDLIASERRYPDRIGEIYRVRHNPD
                     HAWFYVPRMRPDEALVFTVYDSARDGRPRFSAHTAFDDPTTPPGAPARQSIEARAFAF
                     FGPEG"
     gene            1569..3212
                     /locus_tag="VF516_45940"
     CDS             1569..3212
                     /locus_tag="VF516_45940"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEX8115179.1"
                     /translation="MASGSGIDAPTENPRGTRTTPIEQLIPALTVAAHPDLERIGDRF
                     VLDRIAAGREVLLSRGAPEFLGADRALGAPLADRCLSRTPIRLEPTAGGGIRITCEGG
                     TATAIGDVEVAGAIDLPPLGPGDGVPIVLADRVVLVLHLVERGADARGDGDPMGMVGR
                     GLGMTRLRRAIAQVADLEVPVLIRGETGTGKERVARALHDGGPRRSRPFISVNLATIS
                     RELVASELFGAIRGAFTGAARDRDGLFRAARDGTLFLDEVGEASPEVQAALLRVLETG
                     ELYPVGSDTAVTTHARVIAATDADLDGQIRDGRFKAPLFHRLAGYELRVPPLRERPED
                     IGLLFHHFARAERAAIQGDRPAPDDPRAEPWLPASIVVCLLRHRWPGNIRQLRNVARQ
                     LVIASRGRPQLELDAWLAAELTAGAAGPGTSSGHIGTQEAEGEALSAGRSAGPRRRPA
                     EISEPELIAALRASAWDLKAAADHLGIPRASIYDVIARSPNVRTAGDLTPAEIEHCHR
                     ECNGDIDRMAERLEVSRRALGRRVKELGLEPAAAQLRSR"
     gene            3454..4035
                     /locus_tag="VF516_45945"
     CDS             3454..4035
                     /locus_tag="VF516_45945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115180.1"
                     /translation="MSKPISTARNVHDKFGGSNVHPPSGDVANQGQVTRKSNVMSAGA
                     ARPCPPAAVDATGHTQKVTSSPPDTIWVSVDDDGNFSYHPGPDVSANGTVWLVSTGTG
                     SFHVCTYQGETPTDVFDTGPRPYPAARRHGTRYTLKSSVIGNITLRSTTSGSHPSSLG
                     HRRDHQILDGKNGGINVGGGTPPPPPPPPLLAH"
     gene            complement(4084..>5555)
                     /locus_tag="VF516_45950"
     CDS             complement(4084..>5555)
                     /locus_tag="VF516_45950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115181.1"
                     /translation="LRARAMARRATASSDQIAADLAAGRGVLAEAAAIGRSDPAVHVA
                     QGELEQSALRLEIYGAGEIAAPFDAGIAAADRALAIDPDNLAALTLRVGLRRHVAEYR
                     GNLGQDVTELLTGSVTDARRVVELSPSEPAAKLLLARAYRQWAEFRQGRNEDPRTQLQ
                     AALRATDSIAAGGRTYDVHVQVGLIHKVWADYQSDNGKDADDHRTQAIDAYTQALRLD
                     DHRADAWLNLGINYYRRAQQPRGHDAEADLGRALEALERGRVLDPGSYVPYFCEGEVY
                     RMRAERTQARGVDPAPDRARAIELYRKGLTINSQFPLLYNGISMVQVEAARDAFAHGQ
                     DPTRLLDQAYDAAARAIQVAPDQAYGYNNLGDVLLRRAAFERARGRDPRPTAREAIQA
                     FTGALDRFPDDPTFLLNLGAIHSLVAAYELDQARDPRDSLALARRNIESALARDATLD
                     RGKRLLAEVDALEARWRAQPGRRSAPASAERPRGAATPP"
BASE COUNT          769 a         1999 c         1971 g          816 t
ORIGIN      
        1 cttctcctac tacggattct tcacgtggat cccgtcgctg ctcgtcaagc aaggcatgac
       61 gaccaccaag agcttcggct acagcatcat catctatctg gcgcagatcc ccgggtacta
      121 ctcggcggcg ttcctgtccg agaagctcga ccggaaatgg accatcgtcg tgtacatggt
      181 cctgggcggc gtgtcggcgt tcctcatgtc gagtgccggt tccggagcct cgatcacggc
      241 cgcgggattc ctgctgtcgt tcttcatgaa cgggacctac gccgggatct acgcctacac
      301 gcccgagctc tatcccaccg cgttccggac gaccggcatg ggcgtcgcgt cggcgttcgg
      361 tcggctcggt gggctgtccg ccccgatcgt catcggccac gcctacgacc ggatcggctt
      421 cggcggggtc ttcgtcatga ccaccgtcgt cctggtggcc ggcgcgctgg cggtcgccct
      481 gctcggcatt gccaccgccg gcaagagcct cgaggagatc accgcggagg cgctgcgccc
      541 cgccgatccg tgagcggctc acggcgttgc atcagccctc cgggccgaag aacgcgaacg
      601 cgcgcgcctc gatgctctgg cgggccggcg cgccgggcgg cgtcgtcggg tcgtcgaacg
      661 ccgtgtgcgc ggagaaccgc gggcgaccgt cgcgcgccga gtcgtagacc gtgaacacga
      721 gcgcctcgtc gggccgcatc cgcgggacgt agaaccaggc gtgatcggga ttgtggcgga
      781 cgcgatagat ctcaccgatc cggtccggat agcgccgctc ggaggcgatc aggtcgccgg
      841 gtgccaggct gcgcgcatcg acgatcgcga gcgggttgcg ctcggccggc cgcgcgatcg
      901 gccgccagac ctggatgatc gcgaagcggt ggcgcagcag cgcgtctgcc tcggcgggca
      961 ggacatcgcg cacgcgctgc ggcccggacc actcggtgta gtcgttgtgc accagcagca
     1021 ccggctcctg gagctgcttc gcctcgcgct ccgccaggtc ggcgctggag cgtaccgtgt
     1081 ggtcgaacac gacgacccgc cgcgcccccg acacctgggc cacgagcgct tccacctcgg
     1141 gatagtacag ctccgggatc cgcggcttgt cgagcaggtc ggcgacccgc gtcgcgtgct
     1201 cgaccaggac gaagccattg cgctcgagcg cgagctgccc ggcgagccgc cgggcgttgc
     1261 ggatgcgcac ccggcgcggc tcgatcgcgc cgagcttgcg gctgtgcacg ttgcccgggc
     1321 cgtagctctc gtcgagcggc ttgaccccgg tgtcgacgct gtagttgacc tcggcggtga
     1381 cttcgtcgag ctcggagctc tcggtggctg cgaacacgcg ggcagcgtcg cacacggccg
     1441 tcggcatcga gcgaactccg acgcgaggga tgatgtaggt gcgactcttt catggcgccg
     1501 gtgtgcttcg acatccgtga cccgaagcca aattgacagc cgcccgcgtg cggtggacgc
     1561 tgtgcgctgt ggcaagtggg tcagggatcg acgcgccgac ggagaacccc cgtggtacgc
     1621 gcaccacgcc gatcgagcag ctgatcccgg cgctgaccgt tgccgcgcat cccgatctgg
     1681 aacggatcgg tgatcggttc gtcctcgatc ggatcgccgc cgggcgcgag gtgctgctgt
     1741 cgcgcggcgc acccgagttt ctgggcgccg accgcgcgct cggcgcaccg ctcgccgacc
     1801 ggtgcctcag ccgtaccccg atccgcctcg agcccaccgc cggcgggggc atccggatca
     1861 cgtgcgaagg cggcaccgcg accgcgatcg gtgacgtcga ggtcgcgggg gcgatcgatc
     1921 tgcccccgct cggtcccggc gacggcgtgc cgatcgtgct cgccgatcgg gtcgtgctcg
     1981 tgctccacct cgtcgagcgc ggcgccgacg cccgtggtga cggcgatccg atggggatgg
     2041 tcgggcgcgg cctcggcatg acccgcctgc gccgcgcgat cgcgcaggtc gcggacctcg
     2101 aggtacccgt gctgatccgc ggcgagaccg gcaccggcaa ggagcgggtc gcgcgagcgc
     2161 tccacgatgg cggcccccgg cggagccggc cgttcatcag cgtgaacctc gcgacgatct
     2221 cccgggagct ggtggcctcc gagctgttcg gcgcgatccg cggggcattc accggcgcgg
     2281 cgcgcgaccg cgacggcctg ttccgcgccg cgcgcgacgg cacgttgttc ctcgacgagg
     2341 tgggcgaggc gtcgcccgag gtccaggccg cgcttctccg cgtgctggag accggcgagc
     2401 tgtacccggt gggcagcgac acggcggtga cgacccacgc gcgggtgatc gcggcgaccg
     2461 acgccgacct cgatggccag atccgcgacg gccggttcaa ggcgccgctg tttcaccgcc
     2521 tcgccggcta cgagctgcgc gtgccgccgc tgcgcgagcg gccggaggac atcggcctgt
     2581 tgttccacca cttcgcgcgc gcggagcgcg cggcgatcca gggcgatcgg ccggcgccgg
     2641 acgatccacg cgccgagccg tggctgcccg cgtcgatcgt ggtttgcctg ctgcgccacc
     2701 gctggcctgg caacatccgg cagctccgca acgtggctcg tcagctggtg atcgccagcc
     2761 gcggccggcc ccagctcgag ctcgacgcat ggctggcggc cgagctgaca gccggcgcgg
     2821 ccgggccggg tacgagcagc ggccacatcg gcacgcagga ggccgagggc gaggccctgt
     2881 ccgccggaag gtcagcaggg ccccgccggc gaccggccga gatctccgag ccggagctga
     2941 tcgccgcgct gcgcgcgagc gcctgggacc tcaaggcggc cgcggatcac ctgggcatcc
     3001 cgcgcgcgtc gatctacgac gtgatcgcgc ggtccccgaa cgtccgcacc gccggtgacc
     3061 tgaccccggc cgagatcgag cactgccatc gggagtgcaa cggggacatc gatcgcatgg
     3121 ccgagcgcct ggaggtctcg cgccgcgcgc tcggccgccg ggtcaaggag ctgggcctcg
     3181 agccggccgc cgcgcagctg cgatcgcgct gatgcgcaga agttggacag cggcgccgcg
     3241 tcgctggccg gtcggcgcgt cgacgccgag cccggcgcgt cgctgatccg ggtcagtcgt
     3301 ggatcgccgt gcacaacctg gctttgcccg ggcacgaccc gggtgtgcgt ccgggcacgt
     3361 gacttgaact gcgaacttgt cgtccttcac gaatgccagc gccgcacggc ctcttcatcg
     3421 ccgccgcggg ccggcccccc agcagaggaa tttatgtcga aaccgatcag tacggctcgt
     3481 aacgtccatg acaagttcgg cggtagcaac gtccatcccc cgtccggcga cgtcgcaaac
     3541 cagggccagg tcacgcggaa gtccaacgtc atgagtgccg gcgccgcgcg gccttgccct
     3601 ccggccgccg tggacgcgac cggccacacc cagaaggtca cgtcgagtcc cccggacacc
     3661 atctgggttt ccgtcgacga cgacggcaac ttcagctacc accccgggcc agacgtgtcg
     3721 gcgaatggaa cggtctggct ggtgtccacg ggcaccgggt ccttccacgt gtgcacgtac
     3781 cagggcgaaa caccgacgga cgtgttcgac acgggcccgc gtccgtaccc agccgcgagg
     3841 cggcacggta caaggtacac cctcaagtcg agcgtcatcg ggaacatcac gctccgctcg
     3901 acgacctctg gttcgcaccc gagcagcctc ggccatcgtc gcgaccacca gatcctggat
     3961 ggaaagaacg gcggaatcaa cgtcggcggt ggaaccccgc ccccgccccc gcccccgccc
     4021 ctgctggcgc attgacgggc acaggaaccg atcacctcgg cgaccctgca tgcccgggtt
     4081 cgctcacggt ggcgtcgccg cgcccctggg gcgctccgca gaggcggggg ccgatcgacg
     4141 ccccggctgc gcgcgccagc gggcttcgag ggcatcgacc tcggcgagca gccgcttccc
     4201 ccggtccagc gtggcatcgc gagcgagcgc agattcgatg ttccggcggg cgagcgccag
     4261 gctgtcgcgt ggatcgcggg cctgatcgag ctcgtacgcg gcaaccaggc tgtggatcgc
     4321 gcccaggttg agcaggaacg tcgggtcgtc gggaaaccga tcgagcgccc cggtgaacgc
     4381 ctggatcgcc tcgcgtgcgg tcggccgcgg atcgcgcccc cgtgcccgct cgaacgccgc
     4441 acggcggagg agcacgtcgc cgaggttgtt gtagccgtag gcttgatcgg gagccacctg
     4501 gatcgcccgc gccgcggcgt cgtacgcctg gtccagcagc cgcgtcggat cttgcccgtg
     4561 cgcgaaggcg tcccgcgccg cctcgacctg caccatgctg atgccgttgt agagcagcgg
     4621 gaactgcgag ttgatcgtca agcccttgcg gtacagctcg atcgcgcgag cgcgatccgg
     4681 tgccgggtcg acgccgcgcg cctgcgtgcg ctcggcgcgc atccggtaga cctcgccctc
     4741 gcagaaatac gggacgtacg atccgggatc gagcacacgc ccgcgctcca gcgcctccag
     4801 cgcacgcccc agatcggcct cggcatcgtg gccgcgtggc tgctgcgccc gccggtagta
     4861 gttgatcccg aggttcagcc aggcgtcggc gcggtggtca tcgagccgga gcgcctgcgt
     4921 gtacgcatcg atcgcctggg tccggtggtc gtccgcatcc ttgccgttgt cgctctggta
     4981 atccgcccat accttgtgga tcagcccgac ctggacgtgc acgtcgtacg ttcgaccgcc
     5041 ggccgcgatc gagtccgtgg cccggagcgc tgcctggagc tgcgtgcgcg ggtcctcgtt
     5101 gcgcccctga cggaactcgg cccactgccg gtaggcacgt gcgagcaaga gcttggccgc
     5161 gggctccgat ggcgacagct cgacgacgcg gcgggcatcg gtcaccgagc cggtcaacag
     5221 ctcggtgaca tcctggccga ggttccctcg gtactcggcg acgtggcgcc gcaggccgac
     5281 ccgcagcgtg agggcggcga gattgtcggg atcgatcgcg agcgcgcggt cggcggccgc
     5341 gatgccggcg tcgaacggcg cagcgatctc gccggcgccg tagatctcca gccgcagcgc
     5401 actctgctcg agctcgccct gcgcgacgtg aaccgccgga tcgctgcgcc cgatcgcggc
     5461 ggcctcggcg agcacgcccc ggccggcggc gaggtcggcg gcgatctgat cggaggacgc
     5521 cgtggcccgg cgcgccatcg cgcgcgcacg cagga
//
LOCUS       DASVOR010002501         1501 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_465928,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002501 DASVOR010000000
VERSION     DASVOR010002501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465928"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="VF516_45955"
     CDS             <1..580
                     /locus_tag="VF516_45955"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HEX8115182.1"
                     /translation="SGRLATADRKVHLAPMVYLADLGRLAARHAAPAPAGLVVIGRRQ
                     LRSNNSWMHNSQRLVKGPVRCTLLIHPDDAAARGLTSGRLARISIRRGAIDERSESGG
                     RGPAGSAGGEGALPLSIELPVEVSDEVMRGVVSVPHGWGHTRGGVQLQVATARPGQSI
                     NDILDPAVVDELSGTAALFGQPVDVVAVEVAA"
     gene            577..1035
                     /locus_tag="VF516_45960"
     CDS             577..1035
                     /locus_tag="VF516_45960"
                     /inference="COORDINATES: protein motif:HMM:NF013331.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group 1 truncated hemoglobin"
                     /protein_id="HEX8115183.1"
                     /translation="MKPSALFDRIGPDALRAVLTDFYGRVFGDVMIGFLFRGKDRRHL
                     IDREYELTAALLGAPGVTYTGRPMRAAHAQHAIFGGQFERRLQILRETLRDHAVDPDV
                     QQAWIDHQLALRHQITRDRGSECDDTAAAAPRLAIAPPGDLDRPIKLGRK"
     gene            complement(1042..>1501)
                     /locus_tag="VF516_45965"
     CDS             complement(1042..>1501)
                     /locus_tag="VF516_45965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115184.1"
                     /translation="TESETRPEAKPEPRPESRPESRSDARPHGRAMPPPAPVPEARTG
                     PSRLPPHAPVPEPRRAAPAPARAEPPTLEVPTLEVPRLEGSIASFATPRTDPTPAALR
                     PVSGEIRPASRSVDRPNEPVHDAPAKRLDEAALGAAIDRALDEPDLGSPK"
BASE COUNT          180 a          560 c          567 g          194 t
ORIGIN      
        1 ctcgggccgg ctcgccaccg ccgatcgcaa ggtccacctc gcgcccatgg tctacctcgc
       61 cgacctcggc cggctggcgg cccgccacgc cgcgccggcg ccggccgggc tggtggtgat
      121 cggccggcgc cagctgcgca gcaacaacag ctggatgcac aacagccagc gcctggtgaa
      181 gggcccggtg cgctgcacgc tgctgatcca ccccgacgac gcggcggcgc gcggcctgac
      241 ctcgggccgg ctcgcccgga tctcgatccg gcgcggcgcg atcgacgagc gaagcgagtc
      301 gggggggagg ggccctgccg gctccgccgg cggggagggg gccctccctc tttcgatcga
      361 gctgccggtc gaagtcagcg atgaggtgat gcgcggcgtg gtcagcgtgc cgcacggctg
      421 gggccacacc cgcggcggcg tccagctcca ggtcgcgacc gcgcgccccg gccagagcat
      481 caacgacatc ctcgatccgg cggtggtcga cgagctgtcc gggaccgccg cgctgttcgg
      541 ccagccggtc gacgtggtgg cggtcgaggt ggcggcatga agccgagcgc gctgttcgac
      601 cggatcggcc ccgacgcgct gcgcgcggtg ctcaccgact tctacggccg cgtgttcggc
      661 gacgtgatga tcggcttcct gttccgcggc aaggaccgcc ggcacctgat cgaccgcgag
      721 tacgagctga ccgcggcgct gctcggcgca cccggcgtca cctacaccgg ccggccgatg
      781 cgcgccgcgc acgcccagca cgcgatcttc ggcggccagt tcgagcgccg gctgcagatc
      841 ctgcgcgaga ccctgcgcga ccacgccgtc gaccccgacg tccagcaggc gtggatcgac
      901 caccagctcg cgctgcgcca ccagatcacg cgcgaccgcg gctcggagtg cgatgacacc
      961 gcggcggccg cgccccggct ggcgatcgcc ccgcccggtg atctcgatcg gccgatcaag
     1021 ctcggccgca agtagtccgg actacttcgg cgagccgagg tcgggctcgt cgagcgcccg
     1081 atcgatcgcc gcaccgagcg ccgcctcatc gaggcgcttg gctggcgcat cgtgcacggg
     1141 ctcgttcggg cggtcgacgc tgcgcgaagc tggccggatc tccccggaca ccggtctcag
     1201 cgcggcgggg gtagggtcgg tccgcggcgt cgcgaacgaa gcgatgctgc cctcgagtct
     1261 ggggacctcg agggtcggga cctcgagggt cgggggctcc gcacgcgcgg gcgccggtgc
     1321 ggcccgcctg ggctcgggca ccggggcgtg aggaggcagc cggctcggcc cggtccgggc
     1381 ctcgggcacc ggagccggcg gcggcatcgc ccgtccgtgc ggtcgcgcat cggatcggga
     1441 ctccggtcgc gactccggtc ggggctccgg cttcgcctcg ggccgggtct cggactcggt
     1501 g
//
LOCUS       DASVOR010002502         2012 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_465933,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002502 DASVOR010000000
VERSION     DASVOR010002502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465933"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VF516_45970"
     CDS             <1..569
                     /locus_tag="VF516_45970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115185.1"
                     /translation="TGATRAPTVPKTSTAAIPRLPDQPEESRLDRRQRRQQEFAAMFG
                     RLDGESDADYRARILPMITAGLMVPRTRVDEQRKIAQDKAHVTPEQSARLDQAFDKVY
                     GDVLDYANKAIADGQLSPYERNVSGWLEFAGGLGGILHDANGQIGKILDPSQIRAMSD
                     AGFEWGEYLGAKAPWEKLTPPPPPRAAH"
     gene            875..>2012
                     /locus_tag="VF516_45975"
     CDS             875..>2012
                     /locus_tag="VF516_45975"
                     /inference="COORDINATES: protein motif:HMM:NF015497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpvB/TcaC N-terminal domain-containing protein"
                     /protein_id="HEX8115186.1"
                     /translation="MAESRSADNSRRATTSGSVTDNRDDRNSRTTPSTATGAVPTAPT
                     LSLPKGGGAIRGIDEKFSTNLVTGTASFTIPIAASQGRSGFALELGLNYDSGAGNGPF
                     GLGWHMSVPAITRKTDKGLPRYIDAEESDVFVLSGAEDLVPIQADPVLRDGYRIQRYR
                     PRVEGGFARIERWTNQTTGDHHFQVTTRDNVTNVYGRTEGARIADPAAPARVFSWLLE
                     ETRDDRGNAVRYTYKAEDGAGVDPTKTSEVRRFEQTPGAPRFLATAQRYLKRIQYGNR
                     MPLGDHEPAPADPAAWLFEVVFDYGEHDAIAPTPDDVQPRSWQLRADPLSSFRAGFEV
                     RTYRLCRRVLLFHRFAELGGTPCLVRSTDFTYDESPALTYLVKVE"
BASE COUNT          345 a          735 c          645 g          287 t
ORIGIN      
        1 ggaccggggc gacccgcgcg ccgacggtcc cgaagacttc gacggcggcc atcccgcggc
       61 ttcccgacca gcccgaagaa tcgcgcctcg atcgccggca gcgtcgccag caagaattcg
      121 ccgcgatgtt cggccggctc gacggcgagt ccgacgccga ctaccgggcc cggatcctgc
      181 cgatgatcac cgccggcctg atggtgcccc ggacgcgggt cgacgagcag cgcaagatcg
      241 cccaggacaa ggcgcacgtc acccccgagc agtccgcccg gctcgaccag gcgttcgaca
      301 aggtctacgg cgacgttctc gactacgcca acaaggcgat cgccgacggc cagctctcgc
      361 cctacgagcg caacgtctcg ggctggctcg agttcgccgg cggcctcggc ggcatcctgc
      421 acgacgccaa cggccagatc ggcaagatcc tcgacccgtc acagatccgc gcgatgtccg
      481 acgccggctt cgagtggggc gagtacctcg gcgccaaggc cccgtgggag aagctcaccc
      541 cgccgccgcc gccgcgcgcc gcgcactgac gacctcctcg ctgctcgaac ctcgctcccg
      601 ggccacatcg catggccagc acgagcacgt ccacacagtt acgtggccac gccgcaccgc
      661 tcgggatcgg ctcgacccga gcaatcacag gctccgtctc ggcgcgattt aatgcgctgc
      721 gtgcacgatt gcgcgatgcg aaatgctgcg aggccagaca cgggatgtga acgcggaaaa
      781 aataactcac gttgagattg actttacctg cccggtagtt cacatcgtag tctgtcgctc
      841 aacgagcggc ctcattgctg gaagggggag ggcgatggcc gagagccgtt ctgcggacaa
      901 ttcacgccgg gccacaacgt caggctcggt cactgacaat cgggacgacc ggaacagccg
      961 gaccacaccg agcaccgcca ccggggcagt cccgaccgcc ccgaccctgt cgctccccaa
     1021 aggcggtgga gcgatccgcg gcatcgacga gaagttctcg accaacctcg tcaccgggac
     1081 cgcctcgttc acgatcccga tcgcggcctc gcagggtcgc agtggcttcg cgctggagct
     1141 cgggctcaac tacgactcgg gcgccggcaa cggcccgttc gggctcggct ggcacatgtc
     1201 cgttccggcg atcacgcgca agaccgacaa ggggcttccc cggtacatcg atgccgagga
     1261 atcggatgtc ttcgtgctgt ccggggccga ggacctcgtg cccatccagg cggatccggt
     1321 cctgcgcgac ggctaccgga tccagcgcta tcggcctcgc gtggagggcg gattcgcgcg
     1381 gatcgagcgc tggaccaacc agaccaccgg agaccaccac ttccaggtca ccacgcgcga
     1441 caacgtgacc aacgtctacg gcaggaccga gggcgcgcgg atcgccgatc cggcggcgcc
     1501 ggcccgggtg ttctcctggc tcctcgagga gacgcgcgac gatcgtggta acgccgtgcg
     1561 ctacacctac aaggcggagg acggcgcggg ggtcgacccg acaaagacca gcgaggtccg
     1621 gcggttcgag caaacgccgg gcgccccccg cttcctcgcg acagcgcagc gctatctcaa
     1681 gcggatccag tacggcaacc gcatgccgct gggcgaccac gagccggcgc ccgccgatcc
     1741 agccgcgtgg ctgttcgagg tcgtgttcga ttacggtgag cacgacgcga tcgcgccgac
     1801 gcccgacgac gtccagcccc ggtcgtggca gctccgcgcc gatccgctgt cgagctttcg
     1861 cgccgggttc gaggtccgta cctaccggct gtgtcgccgc gtcctcctgt tccatcgctt
     1921 cgccgagctc ggcggcacgc cctgtctggt ccggtccacg gatttcacgt acgacgagag
     1981 ccctgccctc acgtacctcg tgaaggtcga gc
//
LOCUS       DASVOR010002503         3040 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_203170,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002503 DASVOR010000000
VERSION     DASVOR010002503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203170"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VF516_45980"
     CDS             complement(<1..446)
                     /locus_tag="VF516_45980"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829720.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEX8115187.1"
                     /translation="MADVLIFDALRTPRGRGKAGKGGLSQHHPQELLAQTLNHMAERK
                     HLDKRLVDDMAIGCVTQVKEQGACIARNALIAADWPEDVTGVTVNRFCGSGLEAVNMI
                     AGKVGAGFIDAGIGGGVESMSRVPMGSDEAMIDGLNLKLRERLFMVP"
     gene            complement(478..1488)
                     /locus_tag="VF516_45985"
     CDS             complement(478..1488)
                     /locus_tag="VF516_45985"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEX8115188.1"
                     /translation="MLVANPTAHSGKAADWIRKARALLAEAGVPHRFVATEPESRTID
                     RVREAIDDGGARVVIYMGGDGTFAEVAKGIFASHHARDVAMGMLPTGTANDQGKSFGL
                     SAGSGALARNVEVIAAGEIVGCDVGRLVIERGDRTIHRDLFFDSFSIGLGAASLETRN
                     RDRERVGRIPGLGALYRDQLVYAGAVLQRFVESYVVDIKFDVDAVIDGTVHHFEHVLD
                     IILKNTKIFGGEWIFDPRTESDDGKFELVPVTGRRDFGTKLLGTLRHSPVGVDDLQLL
                     GFSHAPAISGARFELTVRSATLPAAQCDGEELPAGERYRVEAVPRALRLIVPRDHVEP
                     GI"
     gene            1681..2823
                     /locus_tag="VF516_45990"
     CDS             1681..2823
                     /locus_tag="VF516_45990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEX8115189.1"
                     /translation="MVALVVSPGFTDEHELLRQTVRQFVQNRVEPQAMEHDQSATFNT
                     ALLAELGALGLIGVTIPEADGGAGMDATASVIVHEELAYSDPGFTLGYLAHALLFVNN
                     FYWAAGPAQRKRYLPGVLSGKLVGGMGMTEPAVGTDVLGMQTTARRDGDGYVLNGRKT
                     FITNGPEGEVFLVYAKLADRITTFVVERDFAGFSTGPKIPKMGMRASTMSELIFEDCR
                     VPADSLLGQEGGGITNMMRNLEIERIGLAAMSLGMARRCLDAMVSYAMERHSFGKPLA
                     EHGQVQRYIGESYAKVEAMRALIYNVAATVGPTSRSRLGTDAAKLFASTAAKEVADAA
                     VQVLGGYGYCTEFRVEQFLRDTKLIEIGGGTLEAHQKNITRDLTQR"
BASE COUNT          442 a         1057 c         1075 g          466 t
ORIGIN      
        1 ggcaccatga acagccgctc gcgcagcttg aggttgaggc cgtcgatcat cgcctcgtcg
       61 gagcccatcg gcacccgcga catcgactcg acgccgccgc cgatgcccgc gtcgatgaac
      121 ccggcgccga ccttgccggc gatcatgttg accgcctcga ggcccgagcc gcagaaccgg
      181 ttgacggtga cgccggtgac gtcctccggc cagtcggcgg cgatcagggc gttgcgcgcg
      241 atgcaggcgc cttgctcctt gacctgggtg acgcagccga tcgccatgtc gtcgaccagg
      301 cgcttgtcga ggtgcttgcg ctcggccatg tggttgaggg tctgggcgag cagctcctgc
      361 gggtggtgct gggacagccc gcccttgccg gccttgccgc ggccgcgcgg cgtgcgcagc
      421 gcatcgaaga tcagtacgtc agccatgagg tcctccaggt ggatgcgggg aaggatgtca
      481 tattcccggc tcgacgtggt cgcgcgggac gatcagtcgc agcgcgcggg gcacggcctc
      541 gacccggtag cgctcgccgg cgggcagctc ctcgccgtcg cactgcgcgg ccggcagggt
      601 cgcgctgcgc accgtgagct cgaaccgcgc gccgctgatc gcgggggcgt ggctgaaccc
      661 gagcagctgg aggtcgtcga ccccgaccgg gctgtggcgc agcgtgccga gcagcttggt
      721 gccgaagtcg cgccggccgg tgaccggcac gagctcgaac ttgccgtcgt cggactcggt
      781 ccgcggatcg aagatccact cgccaccgaa gatcttggtg ttcttgagga tgatgtcgag
      841 cacgtgctcg aagtgatgga cggtgccgtc gatcaccgcg tcgacatcga acttgatgtc
      901 gacgacgtag ctctcgacga agcgctgcag caccgcgccg gcgtagacca gctgatcgcg
      961 gtacagcgcg ccgagcccgg ggatccggcc gacccgctcg cggtcgcggt tgcgggtctc
     1021 gaggctggcc gcgcccaggc cgatcgagaa cgagtcgaag aacaggtcgc ggtggatcgt
     1081 ccgatcgccg cgctcgatca ccaggcggcc gacgtcgcag ccgacgatct cgccggccgc
     1141 gatcacctcg acgttgcgcg ccagcgcgcc gctgccggcc gacagcccga acgactttcc
     1201 ctgatcgttg gcggtgccgg tcggtagcat ccccatcgcg acgtcgcgcg cgtggtgaga
     1261 cgcgaagatt cccttggcga cctcggcgaa cgtgccgtcg ccgcccatgt agatcacgac
     1321 ccgtgcgccg ccgtcgtcga tcgcctcgcg cacccgatcg atcgtccggc tctccggctc
     1381 ggtcgcgacg aaccggtgcg gcacgcctgc ctcggcgagc aacgcgcgcg ccttgcggat
     1441 ccagtcggcg gccttgccgc tgtgcgcggt cgggttggcg accagcacgg ctggtgcgtc
     1501 gttcagccac ggttggatgc cgaccgaccg gttcgtcggt gtaccgtcag ggacggcgtc
     1561 ggtttcagcc acggtggaat ggtactccgc gcgcgcggag tgcgcctcag cctctgcgaa
     1621 ttgccgcaca ggtccgtgcc tgcgttttgg ccggtcgtcg agcttggggt acgctcagcc
     1681 atggtcgccc tggtggtttc tcccggcttc accgacgagc acgagctgct gcgccagacg
     1741 gtgcggcagt tcgtgcagaa ccgcgtcgag ccgcaggcga tggagcacga tcagtcggcg
     1801 acgttcaaca ccgcgctgct cgccgagctc ggcgcgctcg ggctgatcgg cgtcacgatc
     1861 cccgaggccg acggcggcgc cgggatggac gcgaccgcat cggtgatcgt tcacgaggag
     1921 ctcgcgtaca gcgatccggg cttcacgctg ggctacctcg cccacgcgct cctgttcgtc
     1981 aacaacttct actgggcggc gggcccggcg cagcgcaagc gctacctgcc cggtgtgctg
     2041 tcggggaagc tcgtcggcgg catgggcatg accgagcccg cggtcggcac cgacgtgctc
     2101 gggatgcaga ccaccgcgcg ccgcgatggc gacggctacg tcctgaacgg ccgcaagacg
     2161 ttcatcacca acggccccga gggcgaggtg ttcctggtct acgccaagct cgccgaccgg
     2221 atcacgacgt tcgtcgtcga gcgcgacttc gccgggttct cgaccgggcc gaagatcccc
     2281 aagatgggga tgcgcgcgtc gacgatgagc gagctgatct tcgaggactg ccgggtgccg
     2341 gccgacagcc tgctcggcca ggagggcggc ggcatcacca acatgatgcg caacctggag
     2401 atcgagcgga tcgggctggc ggcgatgagc ctgggcatgg cgcggcgctg tctcgacgcg
     2461 atggtcagct acgcgatgga gcgccacagc ttcggcaagc cgctcgccga gcacggccag
     2521 gtccagcgct acatcggcga gagctacgcc aaggtcgagg cgatgcgcgc gctgatctac
     2581 aacgtcgccg cgaccgttgg cccgacctcg cgcagccggc tcggcaccga cgccgccaag
     2641 ctgttcgcgt cgaccgccgc caaggaggtc gccgacgcgg cggtccaggt gctcggcggc
     2701 tacggttact gcaccgagtt ccgcgtcgag cagttcctgc gcgacaccaa gctgatcgag
     2761 atcggcggcg gcacgctcga ggcgcaccag aagaacatca cgcgcgatct gacccagcgc
     2821 tgattgcggc cggcgcgggt cggcacgacg tcgaggcgca tacgtgccgc accgccgggc
     2881 tgctacctgg cgtcggatca cgtcgacgcc cgagccagaa cgtccccatc cgccgttgcg
     2941 ccgccggtct cacccggcgt cgatcacttc gacgccgagc ctccggccgc gggttccgcg
     3001 ccgccggtgg gagctgcggt ccccgcaggg gccgctggag
//
LOCUS       DASVOR010002504         1766 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_465973,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002504 DASVOR010000000
VERSION     DASVOR010002504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1766
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_465973"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VF516_45995"
     CDS             <1..477
                     /locus_tag="VF516_45995"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dimethylallyltransferase"
                     /protein_id="HEX8115190.1"
                     /translation="AAKIDRNDAKRIIRALEVYRLTGEPMSTHQAKHDHRSTPRRYEA
                     RLIGLAPSREALYQAIDHRVDAMIAAGLEREVADLRTRGYRPPLRSQQAIGYAELHDA
                     AEGRVDRARAIELIKRNSRHYARRQVSWYRGDDAIAWHGGPAAVDLADLERYLAGL"
     gene            486..1481
                     /locus_tag="VF516_46000"
     CDS             486..1481
                     /locus_tag="VF516_46000"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEX8115191.1"
                     /translation="MATETAPPPVLTDRMILEFERPIIDLERKISELRGLSTESVDFS
                     SEIRRLEQKARRLQKEVFAELSAQQKVQLARHPGRPYLLDYVGLLMDDFVELHGDRSF
                     RDDPAIVGGLAQFDEWEVLVLGHQKGRNTKDNMHRNFGMARPEGYRKATRLMRLASRF
                     RRPILCFIDTPGAYPGLGAEERGQAEAIAKALQVMASLDCPMISVVIGEGGSGGALAL
                     GVTDRILMLEYSIYSVISPEGCASILWRDPSKISEAASQLKLTAPDLVELGICDEIIP
                     EAPGGAHRNAAQTAAKLRAALKRHLSELVELTPAELIERRYQKFRKLGAFVEQPA"
     gene            1478..>1766
                     /locus_tag="VF516_46005"
     CDS             1478..>1766
                     /locus_tag="VF516_46005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115192.1"
                     /translation="MIYELALVSVVVGAGYWGWYFVRRRPHGTATFGAMQLAAAVLAL
                     LGFLGHRRDIAWLGIPGAIGLGAGACLIVIGPLVRALARRMVVAERMALATR"
BASE COUNT          264 a          623 c          628 g          251 t
ORIGIN      
        1 gcggcgaaga tcgaccgcaa cgacgccaag cggatcatcc gcgcgctgga ggtgtaccgg
       61 ctgaccggcg agccgatgag cacccaccag gccaagcacg accaccgctc gacgccgcgg
      121 cgctacgagg cgcggttgat cgggcttgcg ccttcgcgcg aggcgctgta ccaggcgatc
      181 gaccaccggg tcgatgcgat gatcgcggcg gggctcgagc gcgaggtggc cgacctgcgc
      241 acacggggct accgtccgcc gctgcgctcg cagcaggcaa tcggctacgc ggagctccac
      301 gatgcggcag aaggccgcgt cgatcgggcg cgggcgatcg aattgatcaa aagaaattcg
      361 cgccattacg cacggcgtca ggtgtcgtgg taccgcggtg atgacgcgat cgcctggcac
      421 ggcggacccg ccgccgttga cctggccgac ctggagcggt acctagcagg gctctagcac
      481 ccgccatggc caccgaaacc gcaccgccgc cggtcctgac cgaccggatg atcctggagt
      541 tcgagcggcc gatcatcgac ctcgagcgca agatctccga gctgcgcggg ctgtcgaccg
      601 agtcggtcga cttctcctcg gagatccggc ggctggagca aaaggcgcgc cggctgcaga
      661 aggaagtgtt cgccgagctg tccgcgcagc agaaggtcca gctcgcgcgc caccccgggc
      721 ggccctacct gctcgattac gtcggcctgc tgatggacga cttcgtcgag ctgcacggcg
      781 atcgcagctt ccgcgacgat ccggcgatcg tcggcggcct ggcccagttc gacgagtggg
      841 aggtcctggt gctcggccac cagaagggcc gcaacaccaa ggacaacatg caccgcaact
      901 tcgggatggc gcgccccgag ggctaccgca aggcgacccg cctgatgcgg ctcgcgtcgc
      961 ggttccgccg gccgatcctg tgcttcatcg acacgccggg cgcgtacccc gggctcggcg
     1021 ccgaggagcg cggccaggcc gaggcgatcg ccaaggcgct gcaggtgatg gcgagcctgg
     1081 actgcccgat gatctcggtc gtgatcggcg agggcggctc gggcggcgcg ctcgcgctcg
     1141 gcgtgaccga tcggatcctg atgctcgagt actcgatcta ctcggtgatc tcccccgagg
     1201 gctgcgcgtc gatcctgtgg cgcgatccgt cgaagatcag cgaggcggcg agccagctca
     1261 agctcaccgc gcccgacctg gtcgagctcg ggatctgcga cgagatcatc cccgaggcgc
     1321 cgggcggggc ccaccgcaat gccgcgcaga ccgcggccaa gctgcgcgcg gcgctcaagc
     1381 gccacctgtc ggagctcgtc gagctgaccc ccgcagagct gatcgaacgc cgctaccaga
     1441 agttccgcaa gctcggggcc ttcgtcgagc agccggcgtg atctacgagc tcgcgctggt
     1501 ctcggtcgtg gtcggcgccg ggtactgggg ctggtacttc gttcgccggc ggccgcacgg
     1561 caccgcgacg ttcggcgcga tgcagctcgc cgccgccgtg ctcgccctgc tgggcttcct
     1621 cggccaccgg cgcgacatcg cctggctcgg gatccccggc gcgatcggcc tgggcgcggg
     1681 cgcgtgcctg atcgtgatcg gcccgctcgt ccgcgcgctg gcgcgccgga tggtcgtggc
     1741 cgagcgcatg gcgctcgcga cccggc
//
LOCUS       DASVOR010002505         7068 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_203214,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002505 DASVOR010000000
VERSION     DASVOR010002505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7068
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203214"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VF516_46010"
     CDS             <1..426
                     /locus_tag="VF516_46010"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEX8115193.1"
                     /translation="LRIRGAAGAYRWFKTRAVPIRDASGAVVRWYGSSTDIDQLKRAE
                     DQLSIVLASIRDGFLGVDKDLVVVTINPLAERILGCRAQEVVGHKLLDGVPALRELAD
                     HLPQAPAEFALRAVGGAARIQRSQEGDYLVFLRCPEVTS"
     gene            423..2165
                     /locus_tag="VF516_46015"
     CDS             423..2165
                     /locus_tag="VF516_46015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8115194.1"
                     /translation="MTKRRNSDLRSRAEAKIAQRRKKPSNPSSMAAELEIHRVELELQ
                     NEELRAARDLAEAALARYTEVFDFAPIGYAVLDAGGQIREINHAGAALLGAERALLVG
                     KRFAAAAAAAAAAAARTAFRAFNALLDRARTANRSVQGEVEFLRGGQAWLAQITAAVL
                     PRSEATLMIAFEDVTEARRKEAELERSETALREANRRKDEFLGMLSHELRNPLAPIQT
                     CVATLRLVEPGSEAATRMLDMIERSASHLTRLIEDLLDVTRITSGKIQLQREPVELVG
                     LVSSAVADARLAFERRKLQLEARLPPGELWTRGDPVRLTQVLSNLLTNAQKFTPDGGW
                     VTVSLDRIDTRAVIRVRDTGIGIEPSQIPALFEPFVQASQGLDRSRGGLGLGLAVARG
                     LLELHGGRIAMHSDGPGRGTEVAFELPIESPPGSAQRPLAVAIVAHRRVLIIEDHPDT
                     ACSLETLLAMEGHEVQTALDGAAGLALARTFEPDVVLCDLGLPVMDGFAVARAIRADP
                     VLRDLRLIAVSGYARPDDIARTRSTGFDGHLAKPVTLASLQAALAPAALRSDSPAAAS
                     SGDSILPYSSIWRR"
     gene            complement(2537..3133)
                     /locus_tag="VF516_46020"
                     /pseudo
     CDS             complement(2537..3133)
                     /locus_tag="VF516_46020"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
     gene            3551..4627
                     /locus_tag="VF516_46025"
     CDS             3551..4627
                     /locus_tag="VF516_46025"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEX8115195.1"
                     /translation="MTRPLQGALIGFGFIAEKGHVPAYLTAPDKIQIVAVADVCAARR
                     EKARQSLPHARIYADTKTLLTAEARQLDFVDIATPPCDHAAIAHAAFEHGLHVFCEKP
                     VATSAADARSMLDHALAAKRVFFPSHNYKHAPVIRAIRRVLESGELGPIHLVTLDTFR
                     TTHAKGVADWQPDWRRSRKTSGGGIAMDHGSHTFYLAFDWLASQPTSITAKMSNLSTF
                     DTEDNFSCTVTFPTGIAVAQLTWNSGFRKVIYTIHGANGAIKVEDDEIEIHRKRAGAQ
                     SGQCDVERLSAASHWMDASHAEWFKALQVEFVQAIRTGQWVGPEARDAAMCVQLIETA
                     YASARADGKQLAIGAERAAVVKVG"
     gene            4651..5400
                     /locus_tag="VF516_46030"
     CDS             4651..5400
                     /locus_tag="VF516_46030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEX8115196.1"
                     /translation="MRSDVYFCYATVALFAVAMTLYGVRIAARGVFHSERVSKIGGTA
                     LVGRGVMDATYWAIEPVVRGLAALGVTPNGLTWAALVLGVGAGVALALGWFGLATLLA
                     TMSTIGDILDGQVARLTNSGSNRGELLDAAVDRYTEFAFLAGLVIFLRTSWWWMALAL
                     GATLASFMVSYTSAKAEALQVEAPRGLMRRHERSIYLIVGIGLVPMVGPTLAAHGLPY
                     VTTCLIALGVVAVIGNFAAVLRLMRIARSLR"
     gene            5412..6773
                     /locus_tag="VF516_46035"
     CDS             5412..6773
                     /locus_tag="VF516_46035"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEX8115197.1"
                     /translation="MPQPANQNRRLVTPIPGPRSQALRAREDEHLAPGAQGYALMAGV
                     VVDHAEGTQVTDVDGNQLLDFIGGIAVGALGHSHPTWVEAIQRQAARAAVGSLTSEAR
                     VELLERFARHAPANGVHRLQMYSGGAEAVESALRLAKSHTGKYEFVSCWGGFHGKTMG
                     ALSLMGSTFKDRLGPMVPGSHQVPYADCYRCPVGSSYPSCGIACAEVARRYVKVATAG
                     AVAAVIVEPMQGTAGNIVPPKEFLPAMKSIAEEAGALFIADEMITGLGRTGTWWGVDH
                     TGVAPDIVTIGKAVGGGFPLSALATSDAIAAARPWSNASGSSSSYGGNPLAAAAGAAS
                     LRIIEDEGLVENSRRVGEVMLRELEPWVDRYPFVGTVRGAGMFLAIELVSDKVKKTPL
                     PRKITERIFTECVRRGLLTMSYSASFRIQPPLTTDEATARNGLAILREVFDLVDREAW
                     WKP"
     gene            6761..>7068
                     /locus_tag="VF516_46040"
     CDS             6761..>7068
                     /locus_tag="VF516_46040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115198.1"
                     /translation="METVNFLEALVRALGRGWSARYGVIAPFGVLGVTLTSDVGGKVE
                     VVPSLVRGPAVIWTPLVFTPGTPHYDLYADGVLTRTAVVADAVAHLERWRRARGPAGK
                     V"
BASE COUNT          976 a         2528 c         2578 g          986 t
ORIGIN      
        1 ctgcggatcc gcggcgccgc gggggcctac cgctggttca agacccgcgc ggtgccgatc
       61 cgcgacgcgt cgggcgcggt cgtgcgatgg tacggcagca gcaccgacat cgaccagctc
      121 aagcgcgccg aggaccagct ctcgatcgtc ctggcgtcga tccgcgacgg cttcctcggg
      181 gtcgacaagg acctggtcgt ggtcaccatc aacccgctgg ccgagcgcat cctcgggtgc
      241 cgggcccagg aggtcgtcgg ccacaagctg ctcgacgggg tccccgcgct gcgcgagctc
      301 gccgaccacc ttccccaggc gccggccgag ttcgcgctcc gggcggtggg cggcgcggcg
      361 cggatccagc gcagccagga gggcgattac ctggtgttcc tgcgctgccc ggaggtcacc
      421 tcatgaccaa gcgccgcaac tccgacctgc ggtcccgggc ggaggcgaag atcgcgcagc
      481 gccgcaagaa gccgtccaac ccgagctcga tggccgccga gctcgagatc caccgggtcg
      541 agctggagct ccagaacgag gagctgcgcg cggcccgcga cctggcggag gccgcgctcg
      601 cccgctacac cgaggtgttc gacttcgcgc cgatcgggta cgccgtgctc gacgccggcg
      661 ggcagatccg cgagatcaac cacgccggtg ccgccctgct gggcgccgag cgtgccctgc
      721 tggtcggcaa gcgttttgct gctgctgctg ctgctgctgc tgcggcggcg gcgcggaccg
      781 cgttccgggc gttcaacgcg ctgctggacc gggcacgcac ggcgaaccgg tcggtgcagg
      841 gcgaggtgga gttcctgcgc ggcggccagg cgtggctggc ccagatcacc gcggcggtgc
      901 tgccgcgcag cgaggccacg ttgatgatcg cgttcgagga cgtgaccgag gcccggcgca
      961 aggaggccga gctcgagcgc agcgagaccg cgctgcgcga ggccaaccgg cgcaaggacg
     1021 agttcctcgg catgctgtcg cacgagctgc gcaacccgct cgcgccgatc cagacctgcg
     1081 tcgcgacgct gcgcctggtc gagcccgggt ccgaggcggc gacccggatg ctcgacatga
     1141 tcgagcgctc ggcgagccac ctgacccggc tgatcgagga cctgctggac gtcacgcgga
     1201 tcaccagcgg caagatccag ctccagcgcg agccggtcga gctggtcggc ctggtgtcca
     1261 gcgcggtcgc ggatgcccgg ctcgcgttcg agcgccgcaa gctgcagctg gaggcccggc
     1321 tcccccccgg cgagctatgg acgcggggcg atccggtgcg gctcacgcag gtcctgagca
     1381 accttttgac caacgcccag aagttcacgc cggacggcgg ctgggtgacg gtgtcgctgg
     1441 accggatcga cacgcgggcg gtgatccgcg tgcgcgacac cggcatcggc atcgagccgt
     1501 cgcagatccc cgcgctgttc gagccgttcg tccaggcctc gcagggcctc gaccgctcgc
     1561 gcggcggcct cggcctcggg ctcgccgtgg cgcgcggcct gctggagctg cacggcggcc
     1621 ggatcgccat gcacagcgac gggccggggc gcggcaccga ggtcgcgttc gagctgccga
     1681 tcgagtcgcc gcccgggtcc gcccagcggc ccctcgccgt agcaatcgtg gcgcaccgcc
     1741 gggtcctgat catcgaggat caccccgaca cggcctgcag cctggagacc ctgctcgcca
     1801 tggagggcca cgaggtgcag accgcactcg acggcgccgc gggactcgcg ctcgccagga
     1861 cgttcgagcc cgacgtcgtg ctgtgcgatc tcggcctgcc cgtgatggac gggttcgcgg
     1921 tggcgcgcgc gatccgcgcc gaccccgtgc tccgcgacct ccgcctgatc gcggtcagcg
     1981 gctacgcgcg acccgacgac atcgcgcgga cgcgcagcac cggcttcgac ggccacctcg
     2041 ccaagccggt gacgctcgcc agcctgcagg cggcgctggc cccggccgct ctccggtctg
     2101 actcgccggc cgcggcgtcg agcggggaca gcatcctgcc gtacagcagc atctggcgga
     2161 ggtagtcggc ctgctgcgcg tgggtgttcc ccttctcggc gagccgctgc ttgagctcgc
     2221 cgacctcgtg cacgctgacc cggcgcgcga gcgcggcgct ctcctccagc gcgcgcaccg
     2281 ccgcccacag cgcgcgctcc agctcctcgc tctgctcgcg gaccagggcc tccagcgtga
     2341 aggcgtggcc gacatggcag cggaagtgct ggaacactcc aggctgcgct tcggtcagca
     2401 cgcccgtgca gatcgggcac acgacctcgg cgcgctggcc gagctcggcg ccctcgagct
     2461 ggcggatgaa gcggtcgggc tcgaccgccg gcccggccgg ggtcgcgacc agccgggtga
     2521 gcaggccggg cagctcgagc ggatggacga cgtggtcaac cgggacgcgc tcgatcacgt
     2581 ggctcggcat ctcggaggcg atcgcggtcg tcgggtcctg gacgaccgcg agcccgccgc
     2641 gcgccttgat cgacatcatg ccggcggtgc cgcagtcctg gtacccggac agcacgacgc
     2701 cgatcacgcg cgaaccgtag gcggccgccg cggtccggaa cagcacatcg gcggccgggc
     2761 gatggccgtt gtccttgggg ccgcgcacga cgtcgatcga gcccgggcgc acctggaggt
     2821 ggttgtccgg cggcgcgaca tagatcgcgc cgggccggat cgcctcgcca tgcagcgggt
     2881 gggtcgcggg cagcgggccg cgccggctca gcagctcggg cagcgagccg gcgtggctgg
     2941 acggctggtg caccaccagg aacaccgcgg ccgcgagatc ggcgggcagg tcgccggcca
     3001 gcttcagcag gacctcgatg ctgccggcgg acgcgccgat cgcgacgata tcgtgattca
     3061 ttccggaccg tggacccgga tcccggcggc gccaagccgg gcggcggcgc gctggcacgc
     3121 cagcagcagg catgagaatt cgccgcacgc agtccgcggg cgcacgtgac ggcggacgcc
     3181 acaggggtgt gaccggccgt tcgcggtccg cagccggtcg gcgggagcca gcgctgcgga
     3241 tcaggcagtc gcggtgccgc ggcgccctcc cgcgaggctc tgggccggcg cgtcaatctg
     3301 actcaggccg gccgtgaccg cgtcgtcctg ggcccgtcct ggatcgggtt cacggcggtg
     3361 gttgtcaccg ttcttgcgac cgaaatgccc cacgtttgtc gaaaagctga cagcgatttc
     3421 ggcccctaaa cgtcgaaaaa ccgacatcga tccgtcaggt cccggtacac gggcgtgggg
     3481 gccggactcg gtgtgctccg gttcttgctt ctgtcacgga attgacctac ttcactgccg
     3541 aggcagacgc atgacccgtc cactccaggg tgccctcatc gggttcggat tcattgccga
     3601 gaagggccac gtgccggcct atctcaccgc tcccgacaag atccagatcg tcgcggtcgc
     3661 ggatgtgtgc gctgcccgcc gggagaaggc gcgtcagagt ctccctcacg cccggatcta
     3721 cgccgacacc aagacgctgc tgacggccga ggcccggcag ctcgacttcg tcgacatcgc
     3781 gaccccgccg tgcgaccacg cggcgatcgc ccacgcggcg ttcgagcacg ggctgcacgt
     3841 gttctgcgag aagccggtcg cgaccagcgc ggccgacgcg cggtcgatgc tcgaccacgc
     3901 gctcgccgcc aagcgggtgt tcttcccgtc gcacaactac aagcacgccc cggtgatccg
     3961 ggcgatccgc cgggtcctgg agtcgggcga gctcggcccg atccacctgg tcacgctcga
     4021 cacgttccgg accacccacg ccaagggcgt cgcggactgg cagcccgact ggcggcgcag
     4081 ccgcaagacc tcgggcggcg gcatcgcgat ggaccacggc agccacacgt tctacctggc
     4141 gttcgactgg ctggcgtcgc agccgacctc gatcaccgcc aagatgtcga acctgtccac
     4201 gttcgacacc gaggataact tcagctgcac cgtgacgttc ccgaccggga tcgcggtcgc
     4261 tcagctgacc tggaactccg ggttccgcaa ggtgatctac acgatccacg gcgccaacgg
     4321 cgcgatcaag gtcgaggacg acgagatcga gatccatcgc aagcgcgcgg gggcccagtc
     4381 cggacagtgc gacgtcgagc ggctgtccgc ggcgtcgcac tggatggacg cctcgcacgc
     4441 cgagtggttc aaggcgctgc aggtcgagtt cgtccaggcg atccggaccg gccagtgggt
     4501 cggcccggag gcgcgcgacg cggcgatgtg cgtccagctg atcgagaccg cgtatgcgtc
     4561 ggcgcgcgcc gacggcaagc agctggcgat cggcgcggag cgggcggcgg tggtcaaggt
     4621 gggctgagcg cggcccgcgc gagacacgcc gtgcgctccg acgtctactt ctgctacgcg
     4681 acggtcgcgc tgtttgcggt cgcgatgacc ctgtacggcg tgcggatcgc ggcgcgcggc
     4741 gtgttccaca gcgagcgggt gtcgaagatc ggcggcaccg cgctggtcgg ccgcggggtc
     4801 atggacgcga cctactgggc gatcgagccg gtggtgcgcg ggctggcggc gctcggcgtg
     4861 acccccaacg ggctgacctg ggccgcgctg gtcctcggcg tcggcgccgg ggtcgccctg
     4921 gcgctgggct ggttcgggct ggcgacgctg ctcgcgacga tgtcgacgat cggcgacatc
     4981 ctcgacggcc aggtcgcgcg gctgaccaac agcgggtcca accgcggcga gctgctcgac
     5041 gcggcggtcg accgctacac cgagttcgcg ttcctcgccg gcctggtgat cttcctgcga
     5101 acctcgtggt ggtggatggc gctcgcgctc ggcgcgaccc tggcgtcgtt catggtgagc
     5161 tacaccagcg ccaaggccga ggcgctgcag gtcgaggcgc cgcgcggcct gatgcgccgt
     5221 cacgagcggt cgatctacct gatcgtcggc atcggcctgg tcccgatggt cgggccaacg
     5281 ctcgcggcac acggcttgcc gtacgtcacg acctgcctga tcgcgctcgg cgtcgtggcg
     5341 gtgatcggga acttcgctgc cgtgctccgc ctgatgcgca tcgcccggtc gttgaggtaa
     5401 aagacggcgc catgccgcag cctgcgaacc agaaccgccg cctggtcact ccgatcccgg
     5461 ggcccaggag ccaggcgctg cgcgcgcgcg aggacgagca cctggcgccc ggcgcccagg
     5521 gctacgcgct gatggccggc gtcgtggtcg accacgccga gggcacccag gtcaccgacg
     5581 tcgacggcaa ccagctgctc gacttcatcg gcggcatcgc ggtcggcgcg ctcggccact
     5641 cgcacccgac ctgggtcgag gcgatccagc gccaggcggc gcgcgccgcg gtcggctcgc
     5701 tgaccagcga ggcgcgggtc gagctgctcg agcgattcgc gcgccacgcg ccggccaacg
     5761 gcgtgcaccg gctgcagatg tactcgggcg gggccgaggc ggtcgagagc gcgctgcggc
     5821 tggccaagtc gcacaccggc aagtacgagt tcgtcagctg ctggggcggg ttccacggca
     5881 agaccatggg cgcgctgtcg ctgatgggct cgacgttcaa ggaccggctc gggccgatgg
     5941 tgccgggcag ccaccaggtg ccgtacgccg actgctaccg ctgcccggtc ggatcgagct
     6001 acccgtcgtg cggcatcgcg tgcgccgagg tcgcccgccg ctacgtcaag gtcgcgaccg
     6061 ccggcgcggt cgccgccgtg atcgtcgagc cgatgcaggg caccgccggc aacatcgtgc
     6121 cgccgaagga gttcctgccc gcgatgaagt cgatcgccga ggaggccggc gcgctgttca
     6181 tcgccgacga gatgatcacg ggcctgggcc gcaccgggac gtggtggggc gtcgaccaca
     6241 ccggcgttgc gcccgacatc gtcacgatcg gcaaggcggt cggcggcggg ttcccgctgt
     6301 cggcgctcgc caccagcgac gcgatcgccg ccgccaggcc gtggtcgaac gcgtcgggca
     6361 gctcgtcgag ctacggcggc aacccgctgg cggcggccgc cggcgcggcg tcgctgcgga
     6421 tcatcgagga cgagggcctg gtcgaaaatt cccggcgggt cggcgaggtc atgctgcgcg
     6481 agctcgagcc ctgggtcgat cgctacccgt tcgtcggcac cgtgcgcggc gcggggatgt
     6541 tcctggcgat cgagctggtc agcgacaagg tcaagaagac gccgctgccg cgcaagatca
     6601 ccgagcggat cttcaccgag tgcgtgcggc gcggcctgct cacgatgagc tactcggcga
     6661 gcttccggat ccagccgccg ctcaccaccg acgaggcgac cgcgcgcaac ggcctcgcga
     6721 tcctgcgcga ggtgttcgac ctcgtcgatc gcgaggcctg gtggaaaccg taaacttcct
     6781 cgaggcgctg gtccgcgcgc tcggccgcgg ctggtcggcg cgctacggcg tgatcgcgcc
     6841 gttcggcgtg ctcggcgtga cgctgaccag cgacgtcggc ggcaaggtcg aggtcgtgcc
     6901 gtcgctggtg cgcggccccg ccgtgatctg gaccccgctc gtgttcaccc ctggcacgcc
     6961 gcactacgac ctctacgccg acggcgtgct gacccgcacc gcggtggtcg ccgacgcggt
     7021 cgcccacctc gagcgctggc ggcgggcgcg cgggccggcc ggcaaggt
//
LOCUS       DASVOR010002506         5076 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_436354,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002506 DASVOR010000000
VERSION     DASVOR010002506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5076
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436354"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VF516_46045"
     CDS             complement(<1..1485)
                     /locus_tag="VF516_46045"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8115199.1"
                     /translation="MVGQAAEQIIAGVVLSERLAVTMYGAIHRAQFSGQRNLRGLVVD
                     PKLLAEGSFRLALTDAGAVATAVALDHPSIVPTVAVEEGGPDVVIVTRGVGCYVTVQD
                     LITAARANRSQGGKLAIPVAAAIGKSTIEALAAAHRARVVHGAVHPRSVLIDEDGGVR
                     LGDFVVGRALTTAVAQGADSTMWRGLAGYLAPELVVGEDPSPGADVFAVGAMLFTMLS
                     GDVPPGSLHVTPAVERLVQRALDTDLTRRYRSASDLLENLLEAFEDDRWELADRAEIV
                     SAAGLSQSDTNVDEATEDLLASLATPGAVQVTPIRPSADIRAQAIASHHHQHHIDTPP
                     GNRLDALLADLDDSRELTAVDDDRPFVRDPISEVIAKHPRRSEAIAQLRHRVPSLDDP
                     DDDTPLPPPMRESDQIPAARQGAATAAGDEAAAMSALAGLDEPRDARRPLSIDEPRGG
                     VNGQALPGQRGRERPLSIDEPARLVSSAAEQAVQAADKLEQAARR"
     gene            complement(1517..2452)
                     /locus_tag="VF516_46050"
     CDS             complement(1517..2452)
                     /locus_tag="VF516_46050"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEX8115200.1"
                     /translation="MLAELEPLAMSAAGYGFVLALGLLWGSFANVCIYRWPAGRSVVT
                     PGSHCLACQAPIRWYDNVPVLAWLWLRGRCRACKASFSPRYLVVEAATGALFGVAWWA
                     TVIAPALFEPFSARLLHFAIDAAFCFVMVVITFIDIDHKLILNKVTVPSIALAYLASF
                     AWPGRQWYDGVVGAVVGYGLPWAIGEIYYRITRREGLGLGDGMLLAVVGALLGWRGVV
                     VALFGGSVIGSVIGITAVLLGRVGELGGEPSPTEPAAPVGDGDPGVAGAAPGEPEKPS
                     VMRTEVPFGPFLAMAAVLYLFAEPWVQLHFHLPGG"
     gene            2525..3226
                     /locus_tag="VF516_46055"
     CDS             2525..3226
                     /locus_tag="VF516_46055"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115201.1"
                     /translation="MTATAVRTLATRVARPARRRAVVVSAWTGEPAVESTDRRAIDFE
                     YERQRHAGFVGRTDLLARLDRQLWNRLRRSGWSASELDAQLRVPEGLTALAVTPDGRH
                     VVSASYDKTLKVWKLGPGRAVATLAGHSNWVTACVVTPDGRHVVSASEVQTLKVWEVG
                     SRRAVVTLEGHISGVSACAVTPDGQHVVSASRDQTLKVWELGSGHTVVTLEGHTSGVT
                     VGGGARRQTRGLGIA"
     gene            3207..3725
                     /locus_tag="VF516_46060"
     CDS             3207..3725
                     /locus_tag="VF516_46060"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115202.1"
                     /translation="MVSASRDQTLKVWELGSGRAVAMLAGHTRLVTALAVTPDGRRVV
                     SASYDRTLKVWDLESYACRFTHRGDAPYLAVAVTATTIVAGDRAGVVWFLDVPQSLVS
                     SIDPPSAPEPPMTSTISPVAAPAPAARPSPKVDIGIVTIRDDEFRAVLATFPDKVGTL
                     HGARRARTPRGT"
     gene            3749..4558
                     /locus_tag="VF516_46065"
     CDS             3749..4558
                     /locus_tag="VF516_46065"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4351 domain-containing protein"
                     /protein_id="HEX8115203.1"
                     /translation="MLVVGIAGGRPSSDVTLGDVVVSTRIHDFTVEARKAGEPPAYAV
                     TGGPIDKALAALVANLPAREDELGDWTAGLQAQPPVSWTQDGQLYGPPDWQRELRDML
                     ERHHSPQATPRARVRRWPDRVERSAGEGSGAADPLAPDRAQPARDRDGVRRLAAPLLV
                     HFKLGTGDHATDPEDDMSAEIRAWFEDYKQKLRAEANTEGREEGRKEGIKNGIKEGHR
                     TLLLRLLHARFGELPEAVVARIEAADIADLERWGVRVLGAQTLDEVLDDPS"
     gene            4703..>5076
                     /locus_tag="VF516_46070"
     CDS             4703..>5076
                     /locus_tag="VF516_46070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115204.1"
                     /translation="MIRFTVLVLCAAACGRTRGVQDQDLPGLVVEARKPEAPIDVGRA
                     ARDPGELGRALARPYRVVLAALGPHTAGVNSAITVTEGGKPVSELSDHAQIDNGTGDA
                     YHAIYTNSADYGRETTFVDGTL"
BASE COUNT          773 a         1829 c         1815 g          659 t
ORIGIN      
        1 ccggcgggcc gcctgctcga gcttgtcggc cgcctgcacg gcctgctcgg ccgcgctcga
       61 caccagccgc gccggctcgt cgatcgagag gggacgttcc cgtcccctct ggcccggcaa
      121 agcctggcca ttcacccctc cgcgcggctc gtcgatcgag agggggcgtc tcgcgtcgcg
      181 cggctcgtcc agacctgcca gcgcgctcat cgcggcggcc tcgtcgccgg cggcggtggc
      241 cgcgccctgc cgggcggccg ggatctggtc gctctcgcgc atcggcggcg gcagtggggt
      301 atcgtcgtcg ggatcgtcga gcgacggcac ccggtggcgg agctgggcga tcgcctcgga
      361 gcgccgcggg tgcttcgcga tcacctcgct gatcgggtcg cgcacgaacg gccggtcgtc
      421 gtcgaccgcg gtcagctcgc gcgagtcgtc gaggtcggcg agcagcgcgt cgagccggtt
      481 gcccggcggg gtgtctatgt ggtgctggtg atggtgcgac gcgatggcct gggcccggat
      541 gtcggcgctc gggcggatcg gcgtgacctg gaccgcgccg ggcgtcgcga gggacgcgag
      601 caggtcctcg gtcgcctcgt cgacgttggt gtcggactgc gacagcccgg cggcgctgac
      661 gatctcggcg cggtcggcga gctcccagcg atcgtcctcg aacgcctcga gcaggttctc
      721 gagcaagtcg ctcgcgctgc gatagcgccg ggtcaggtcg gtgtcgagcg cgcgctggac
      781 cagccgctcg accgccggcg tcacgtgcag gctgcccggc ggcacgtcgc ccgacagcat
      841 cgtgaacagc atcgcgccga ccgcgaacac gtcggcgccg ggcgacggat cctcgccgac
      901 caccagctcg ggcgcgaggt agccggccag gccgcgccac atcgtggagt cggcgccctg
      961 ggcgaccgcg gtggtcagcg cgcggccgac cacgaagtcg ccgagccgga ccccgccgtc
     1021 ctcgtcgatc agcacgctgc gcgggtggac cgcgccgtgc acgacccggg cgcggtgcgc
     1081 cgcggcgagc gcctcgatcg tgctcttgcc gatcgccgcc gcgaccggga tcgccagctt
     1141 gccgccctgg ctgcggttgg cgcgcgccgc cgtgatcagg tcctgcaccg tgacgtagca
     1201 cccgacgccg cgcgtgacga tcacgacgtc ggggccgccc tcctcgaccg ccacggtcgg
     1261 gacgatgctc gggtgatcga gcgcgaccgc ggtggcgacc gccccggcat cggtcagcgc
     1321 gaggcggaac gagccctcgg ccaggagctt ggggtcgacc accaggccgc gcaggttgcg
     1381 ctggccggag aactgggcgc ggtggatcgc gccgtacatc gtgaccgcga gccgctcgct
     1441 gagcacgaca ccggcgatga tctgctcggc ggcttggccc accacctggg gactatagca
     1501 acgaccagcc gccggctcac ccgccaggta ggtggaagtg gagctgaacc cacggctcgg
     1561 cgaacaggta cagcaccgcc gccatcgcga ggaacgggcc gaacggcacc tcggtgcgca
     1621 tgacggaggg tttctccggt tcccccgggg cggcacccgc gaccccggga tcgccatcgc
     1681 ccaccggcgc agccggctcc gtgggggacg gctcaccgcc cagctcaccc acccggccga
     1741 gcagcaccgc ggtgatgccg atgaccgacc cgatcaccga gccgccgaac agcgcgacca
     1801 cgacgccgcg ccagccgagc agcgcaccga ccaccgcgag cagcatgccg tcgccgagcc
     1861 ccaggccctc gcggcgcgtg atccggtagt agatctcgcc gatcgcccac ggcaggccat
     1921 agccgaccac cgctcccacc acgccgtcgt accactgccg acccggccac gcgaacgacg
     1981 ccaggtacgc cagcgcgatc gacgggaccg tgaccttgtt caggatcagc ttgtggtcga
     2041 tgtcgatgaa ggtgatcacc accatcacga agcagaacgc ggcgtcgatc gcgaagtgca
     2101 acagccgcgc gctgaatggc tcgaacagcg ccggggcgat caccgtcgcc caccacgcga
     2161 ccccgaacag cgcgccggtc gccgcctcga cgaccaggta gcgcggcgag aacgacgcct
     2221 tgcacgcccg gcaccgcccg cgcaaccaca gccacgccag caccggcacg ttgtcgtacc
     2281 agcggatcgg cgcctggcag gccaggcagt gcgagcccgg cgtcaccacc gagcggccgg
     2341 ccggccagcg atagatgcac acgttggcga agctgcccca caaaagcccc agcgcgagga
     2401 cgaacccgta gccggccgcc gacatcgcca ggggttcgag ctcggccagc accgcagagt
     2461 tacagtagcc gctccatcgc ccgcaggcga gccggccgcc ggtggttcga ggatgccagg
     2521 ggcgatgacg gcgaccgccg tgcggacgct cgcgacgcgc gttgcccggc cagcgcgccg
     2581 ccgcgcagtg gtagtttcag cgtggaccgg ggagcccgcg gtggagagca cagaccggcg
     2641 agcgatcgac ttcgagtacg agcggcagcg acatgccggg ttcgtcggac gcacggatct
     2701 gctggcccgg ctcgatcggc agctgtggaa ccggctccgc cggtcggggt ggagcgcgag
     2761 cgagctggac gcgcagctcc gtgtgccgga gggcctgacc gcgttggcgg tcacgccgga
     2821 cggccggcac gtggtctcgg cgtcgtatga caagacgctc aaggtctgga agctggggcc
     2881 cgggcgggcg gtggccacac tcgcaggcca cagcaactgg gtgacagcgt gcgtggtgac
     2941 gccggacggc cggcatgtgg tctcggcgtc ggaagtccag acgctcaagg tctgggaggt
     3001 ggggagccga cgcgcggtgg tcacactcga aggccacatc tccggggtga gcgcgtgcgc
     3061 ggtgacgccg gacggccagc acgtggtctc ggcatcgcgg gaccagacgc tcaaagtctg
     3121 ggagctgggg agcgggcaca cggtggtcac actcgaaggc cacacctccg gggtaaccgt
     3181 tggcggtggc gccagacggc agacacgtgg tctcggcatc gcgtgaccag acgctcaagg
     3241 tctgggagct ggggagcgga cgcgcggtgg ccatgcttgc aggccacacc cgcttggtga
     3301 ccgcgttggc ggtgacgccg gacggccggc gcgtggtctc ggcgtcgtat gaccggacgc
     3361 tcaaggtctg ggacctcgaa agctacgcat gccgcttcac ccaccgcggc gacgccccgt
     3421 acctcgcagt cgccgtcacc gcgaccacca tcgtcgctgg cgaccgtgct ggtgtcgtct
     3481 ggttcctcga cgtgccgcag tctctcgtct cctcgatcga cccgccctcc gcgccggagc
     3541 cgcccatgac gtccacgatc tcgcccgtcg ctgcccctgc gcctgctgcc cgcccttcgc
     3601 cgaaggtgga catcggcatc gtgacgatcc gcgacgacga gtttcgcgcc gtgctcgcca
     3661 cgttcccgga caaggtcggc accctccacg gcgcccgccg cgcgaggacg ccgcgcggga
     3721 cctgatcgac gatctcggcc cccagctcgt gctcgtggtc ggcattgccg gcgggcgtcc
     3781 gtccagcgac gtcacgctcg gcgacgtcgt cgtctcgacg cggatccacg acttcaccgt
     3841 cgaggcgcgc aaggcgggcg agccgcccgc gtacgccgtg accggcggcc ccatcgacaa
     3901 ggccctggca gccctggtcg ccaacctccc ggcgcgcgag gacgagttgg gcgactggac
     3961 ggccggcctg caggcgcagc cgccggtgag ctggacgcag gacgggcagt tgtacggacc
     4021 gccggactgg cagcgcgagc tgcgcgacat gctggagcgc caccacagcc cccaggccac
     4081 gccccgcgcc cgtgtacgtc gctggcccga tcgcgtcgag cgatcggctg gtgaaggatc
     4141 cggagctgct gatcccctgg ctccagaccg cgcgcagcct gctcgcgatc gagatggagt
     4201 ccggcggctg gcggcgcctc tgctggtaca cttcaagctc gggactggcg accacgcgac
     4261 ggacccagag gacgacatga gcgcagaaat tcgagcatgg ttcgaagact acaagcagaa
     4321 gctgcgcgcc gaggcgaaca cagaagggcg cgaggaaggg cgcaaggaag ggatcaagaa
     4381 tgggatcaag gaagggcacc gcaccttgct gctgcgcctg cttcatgctc gattcggcga
     4441 gctacccgag gctgtcgtcg cacgcatcga ggcggctgac atcgccgacc tcgagcgctg
     4501 gggcgtgcgc gtgctcggcg cccagacgct cgacgaggtg ctcgacgatc cgagctgatc
     4561 gatcaccggg ctgaccgatc actgctcacc gcatccacct atgtggtcga gatgcaggta
     4621 ctcaaggtcg aggtgttcgc aaagacgcgc cgccccggcc gaccagttga ccacgttcgt
     4681 cggcaaagct aagaaacccg ggatgatccg gttcaccgtc ctggtgctgt gcgccgccgc
     4741 ctgcggccgg acgcgcggtg tgcaggatca ggacctcccc ggcctggtgg tggaggcgcg
     4801 caagcccgag gcaccgatcg acgtggggcg cgccgcccgc gatccggggg agctcggccg
     4861 ggcgctggcg cggccgtacc gggtggttct cgcagcgctc ggcccccaca ccgccggggt
     4921 caacagcgcg atcaccgtga ccgagggcgg caagccggtc agcgagctgt ccgaccacgc
     4981 ccagatcgac aacggcaccg gcgacgccta ccacgcgatc tacaccaact ccgccgacta
     5041 cggccgcgag accacgttcg tcgacggcac gctgta
//
LOCUS       DASVOR010002507         1528 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_495739,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002507 DASVOR010000000
VERSION     DASVOR010002507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1528)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1528)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1528
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495739"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VF516_46075"
     CDS             complement(<1..251)
                     /locus_tag="VF516_46075"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor"
                     /protein_id="HEX8115205.1"
                     /translation="MIDRGALDRMMAAAADGDRAAIAPLFHALWPVVVRYATRLVGDA
                     SLAEDCAQDALVQLFGQLDRYDRGRDALTWALTLATWQAR"
     gene            complement(298..441)
                     /locus_tag="VF516_46080"
     CDS             complement(298..441)
                     /locus_tag="VF516_46080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115206.1"
                     /translation="MRIIKALSLTAALLLPVAALTAPLAGRCSDPACCPHCPDCPLCD
                     HGK"
     gene            518..>1528
                     /locus_tag="VF516_46085"
     CDS             518..>1528
                     /locus_tag="VF516_46085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEX8115207.1"
                     /translation="MAAPAATSTPLLEIRDLVTEFRTGDGAVRAVDGVSFEIAPRTTL
                     GVVGESGSGKSVTALSIMRLVASPPGRIASGSIRYAGRDLLALRPAEMRAIRGNRIAM
                     IFQEPMTSLNPVFTVGDQVAEAVRVHQHRSRREARAVAIDMFRRVGIPSPEDRVDAYP
                     HQLSGGMRQCVMIAMALACKPDLLIADEPTTALDVTIQAQILDLLRSLQRELGMSILL
                     ITHDLGVVAETCDEVVVMYAGRIVERAPTEALFAAPRHPYTAGLLRSVPSYGEGTAVG
                     DRTRLQEIRGMVPALSELPPGCKFADRCPAVAERCRAEEPPLVVLGASRVRCHFPLDP
                     PAG"
BASE COUNT          196 a          535 c          591 g          206 t
ORIGIN      
        1 cgggcctgcc aggtcgccag cgtcagcgcc caggtcagcg cgtcgcgccc gcggtcgtag
       61 cggtcgagct gaccgaacag ctggaccagc gcgtcctgcg cgcagtcctc ggcgaggctc
      121 gcgtcgccga ccaggcgggt cgcgtagcgc acgacgaccg gccacagcgc atggaacagc
      181 ggcgcgatcg ccgcgcgatc gccgtccgcg gcggcggcca tcatgcggtc gagcgcgccc
      241 cggtcgatca tcctcgctgt gccctgccgg cgatcatgtc atgcggccgg cgcgggttca
      301 cttgccgtga tcgcacagcg ggcagtccgg gcagtgcggg cagcaggcag ggtcgctgca
      361 gcgcccggcg agcggcgcgg tgagcgcggc gacggggagg agcaaggcgg cggtgaggct
      421 gagagctttg atgattcgca tgcccggtat gggcgcgagc gggcaaagcg tgacagcgaa
      481 attgctgccc cgcgcgccgc gctggtaggc tgggcccatg gcggcgccgg ccgcaacgtc
      541 caccccgctg ctggagatcc gcgatctggt gacggagttc cgcaccggcg acggggcggt
      601 ccgggcggtc gacggggtgt cgttcgagat cgcaccgcgc accacgctcg gggtggtcgg
      661 cgagtcggga tccggcaaga gcgtgacggc gctgtcgatc atgcggctgg tcgcgtcgcc
      721 gcccggccgg atcgcgtcgg ggtcgatccg ctacgccggc cgcgacctgc tcgcgctgcg
      781 gccggccgag atgcgcgcga tccgcggcaa ccggatcgcg atgatcttcc aggagccgat
      841 gacgtcgctc aacccggtgt tcacggtcgg cgaccaggtc gccgaggcgg tccgggtgca
      901 ccagcacagg tcgcgccgcg aggcccgcgc ggtggcgatc gacatgttcc ggcgggtcgg
      961 catcccgtcg cccgaggacc gggtcgacgc gtacccgcac cagctgtcgg gcgggatgcg
     1021 gcagtgcgtg atgatcgcga tggcgctggc gtgcaagccg gacctgctga tcgccgacga
     1081 gccgaccacg gcgctcgacg tgacgatcca ggcccagatc ctcgacctgt tgcgctcgct
     1141 gcagcgcgag ctcgggatga gcatcctgct catcacccac gacctcggcg tggtggccga
     1201 gacctgcgac gaggtggtcg tgatgtacgc ggggcgcatc gtcgagcgcg ccccgaccga
     1261 ggcgctgttc gcggcgccgc gccatcccta cacggcgggc ctcctgcgct cggtgccgag
     1321 ctacggcgag gggacggcgg tgggcgaccg cacccggctg caggagatcc gcggcatggt
     1381 gccggcgctg agcgagctgc cgccgggctg caagttcgcc gaccgctgcc cggcggtcgc
     1441 cgagcgctgc cgcgccgagg aaccgccgct ggtcgtgctc ggcgcctcgc gggtgcgctg
     1501 ccactttccg ctcgatccgc cggccggg
//
LOCUS       DASVOR010002508         1994 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_320353,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002508 DASVOR010000000
VERSION     DASVOR010002508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320353"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1115
                     /locus_tag="VF516_46090"
     CDS             120..1115
                     /locus_tag="VF516_46090"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HEX8115208.1"
                     /translation="MVLGALPTPLTEARGLREALGGPRRCPRIFLKRDDLTGLALGGN
                     KVRKLEYLIADARRRGATSVITTGAVQSNHARMTAAAARAAGLGVTLVLTTREASPAR
                     QGNYLLDRIFGATIHLIPAGPDPLAAVAPDEEDRVREVVDDERRRGGQPYVIPVGGSS
                     GVGALGYASGTLELVEQLAEAGLSPSYLYYASGSRGTQAGLELGARLFSPPYRLRGIA
                     VSGGEPDKRVRAARIAGEAAALLGVDVAIRPDELSTHQGHIGDGYGIATPGGREAIRL
                     LAEGDGVLLDPTYTAKAMAALIADIRAGAVAPDDTVVFLHTGGAPALFAHAGLFD"
     gene            complement(1116..>1994)
                     /locus_tag="VF516_46095"
     CDS             complement(1116..>1994)
                     /locus_tag="VF516_46095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase family protein"
                     /protein_id="HEX8115209.1"
                     /translation="RIVCMYRWDPELAARLIARERLTSIVAPAAITGDLVRVARAGGH
                     DLSSLITVGGGGAPRAPEQVRQIAASFGNALPNIGWGMTETNAIGTSISGADYLARPE
                     SSGRCSQVLDLSIRDDAGRELPTGQRGELWVRGTSVFRGYWNLPEATAAAFVGDWFRT
                     GDGAYLDGEGFVFIVDRLKDLIIRGGENIGCGHVEAALLMHPAVREASVYAVPDDRLG
                     EEVGATIYADGALDEAELRAFLAGHLARYELPRYIVRSPGPLPRTPSGKILKRQIRDE
                     AIAQLAAAAPAGGRPA"
BASE COUNT          243 a          718 c          773 g          260 t
ORIGIN      
        1 gttcatggcg cgcacccgtc gtattccggg gagttccgga ccgtgcgccg gcgcgcagcg
       61 gtactctcgg catgatgtcg tactccgcat ctcgttcgtt gtttgccacg atcccgcgcg
      121 tggtcctcgg cgcgctgccg accccgctca ccgaggcgcg cggcctgcgg gaagcgctcg
      181 gcggaccgcg gcggtgtccg cgcatcttcc tcaagcgcga tgacctgacc ggcctggcgc
      241 tcggcgggaa caaggtccgc aagctggagt acctgatcgc cgacgctcgc cgccgcggcg
      301 cgacgtcggt gatcaccacg ggcgcggtgc agtcgaacca cgcgcggatg accgccgcgg
      361 ccgcgcgcgc cgccgggctg ggggtcacgc tggtgctgac cacgcgcgag gcatcgccgg
      421 cgcgccaggg caactacctg ctcgaccgga tcttcggcgc gacgattcac ctgatcccgg
      481 cggggcccga tccgctcgcg gcggtcgcgc ccgacgagga ggaccgggtg cgcgaggtcg
      541 tcgacgacga gcggcgccgc ggcgggcagc cgtacgtgat cccggtgggc gggtcgagcg
      601 gcgtgggcgc gctcggctat gccagcggca cgctcgagct ggtcgagcag ctggccgagg
      661 cggggctgtc gccgtcgtac ctctactacg ccagcggatc gcgcggcacg caggccgggc
      721 tggagctcgg cgcccggctg ttctcgccgc cgtaccggtt gcgcgggatc gcggtgagcg
      781 gcggcgaacc ggacaagcgc gtgcgggcgg cgcggatcgc cggcgaggcc gcggcgctgc
      841 tcggcgtcga cgtggcgatc cggcccgacg agctgtccac gcaccagggc cacatcggcg
      901 acggctacgg catcgccacg ccgggcggcc gggaggcgat ccggctgctc gccgagggcg
      961 atggcgtgct gctcgatccg acctacaccg cgaaggcgat ggcggcgctg atcgccgata
     1021 tccgggcggg cgccgtggcg ccggacgaca ccgtcgtgtt cctccacacc ggcggtgcgc
     1081 cggcgctgtt cgcgcacgcc gggctgttcg actgactacg ccgggcgtcc gccggcgggg
     1141 gccgccgccg cgagctgtgc gatcgcctcg tcgcggatct ggcgcttgag gatcttgccc
     1201 gacggggtgc gcggcagcgg gccgggcgag cgcacgatgt agcgcggcag ctcgtagcgc
     1261 gcgaggtggc cggcgaggaa cgcgcggagc tcggcctcgt cgagcgcgcc gtcggcgtag
     1321 atcgtcgcgc cgacctcctc gccgagccgg tcgtcgggca ccgcgtacac cgaggcctcg
     1381 cggaccgccg ggtgcatcag cagcgcggcc tcgacgtggc cgcagccgat gttctcgccg
     1441 ccgcggatga tcaggtcctt gagccggtcg acgatgaaca cgaagccctc gccgtcgagg
     1501 taggccccgt cgccggtgcg gaaccagtca ccgacgaacg ccgcggcggt cgcctcgggc
     1561 aggttccagt agccgcggaa caccgacgtg ccgcgcaccc acagctcgcc gcgctggccg
     1621 gtcggcagct cgcggcccgc gtcgtcgcgg atcgagaggt cgagcacctg ggagcaccgg
     1681 ccggagctct ccgggcgggc gaggtagtcg gcgccggaga tcgaggtgcc gatcgcgttg
     1741 gtctcggtca tgccccagcc gatgttgggc agcgcgttgc cgaagctcgc cgcgatctgg
     1801 cggacctgct cgggcgcgcg cggggcgccg ccgccgccga cggtgatcag cgagctcagg
     1861 tcgtggccgc ccgcccgggc cacgcgcacc aggtcgccgg tgatcgccgc cggcgcgacg
     1921 atcgaggtca gccgctcgcg cgcgatcagg cgcgcggcga gctcggggtc ccagcggtac
     1981 atgcacacga tgcg
//
LOCUS       DASVOR010002509         1668 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_203251,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002509 DASVOR010000000
VERSION     DASVOR010002509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1668
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203251"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1668
                     /locus_tag="VF516_46100"
     CDS             <1..>1668
                     /locus_tag="VF516_46100"
                     /inference="COORDINATES: protein motif:HMM:NF023400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115210.1"
                     /translation="ELGADNQVPTAVVIELAAPVIDREAIGSASPKTNLKIAPAVDGE
                     LSHTGVSELTFTPSRPFDFDTTYQIELTGVDTVDGPVAPAAGETWSYSFKTPAFKFLG
                     WAPSSIDVEHHKLAMDLAFSGAVLPNVATAALRITVDGKAPAGIAAQRSRDPSHVVIT
                     LSDPKIALGAKLAMATRGALTSRTGVRAPAASAEYIVSADKAVSIKTAVMTEGATGFY
                     LEVVCDDGAAPKGNRGFYEGEGYYNLSERCQLTDESIKHIHFDPPVGKPYITAGRAGF
                     RVFGGFKRGVYKVKIDGGATSVDGGVVLAPFTRSFTVAARKPQLGFATSGRYLPRSAW
                     THLGIKHINVDTANLVIRRVPAENLVFWLGEATDAVTERTSDVILKKTIPLRGDPDVQ
                     ATSWLDVEPLLPKTTRGVLELKLVGVGAEGTARLLLTNMSLVAKKWSAPGKPAAQTVQ
                     VWALDMDSADPLSGVEVSLVRKSGKVVATCTTSGGDGCTLAARTDDDPDDAVPFAVIA
                     KKGDDLTYIRYQDLRADVAESSTSGVEVVADAPYHAAIYPDRDVFRPG"
BASE COUNT          273 a          552 c          629 g          214 t
ORIGIN      
        1 gagctcggcg ccgacaacca ggtgccgacc gcggtggtga tcgagctggc ggcgccggtg
       61 atcgaccgcg aggcgatcgg ctcggcgagc ccgaagacca acctgaagat cgcgccggcg
      121 gtcgacggcg agctgagcca caccggggtg tcggagctga cgttcacgcc gagccggccg
      181 ttcgacttcg acacgacgta ccagatcgag ctcaccgggg tcgacacggt cgacgggccg
      241 gtcgcgcccg cggcgggcga gacgtggagc tactcgttca agaccccggc gttcaagttc
      301 ctgggctggg cgccgagcag catcgacgtc gagcaccaca agctcgcgat ggatctcgcg
      361 ttctcgggcg cggtgctgcc caacgtcgcc acggccgcgc tgcggatcac ggtcgacggc
      421 aaggcgccgg ccgggatcgc agcccagcgc agccgcgacc cgagccacgt cgtgatcacg
      481 ctcagcgatc cgaagatcgc gctcggggcg aagctcgcca tggcgacccg gggcgcgctg
      541 acgtcgcgga ccggcgtgcg ggcgccggcg gcgagcgccg agtacatcgt gtcggcggac
      601 aaggcggtgt cgatcaagac cgcggtgatg accgagggcg cgaccgggtt ctacctcgag
      661 gtggtgtgcg acgacggcgc ggcccccaag ggcaaccgcg ggttctacga gggagagggc
      721 tactacaacc tgtccgagcg ctgccagctc accgacgagt cgatcaagca catccacttc
      781 gacccgccgg tcggaaagcc gtacatcacc gccgggcgcg ccgggttccg ggtgttcggc
      841 gggttcaagc gcggcgtgta caaggtgaag atcgacggcg gcgcgacctc ggtcgacggc
      901 ggcgtggtgc tggcgccgtt cacccggtcg ttcacggtgg cggcgcgcaa gccgcagctg
      961 gggttcgcga cgagcgggcg ctacctgccg cgcagcgcgt ggacccacct ggggatcaag
     1021 cacatcaacg tcgacaccgc gaacctggtc atccgccggg tgccggcgga gaacctggtg
     1081 ttctggctcg gcgaggcgac cgacgcggtg accgagcgaa ccagcgacgt gatcctcaag
     1141 aagacgatcc cgctgcgcgg cgatccggat gtccaggcga cgagctggct cgacgtcgag
     1201 ccgctgctgc cgaagacgac ccggggcgtg ctggagctca agctggtcgg ggtcggcgcc
     1261 gagggaacgg cgcgcctgct gctgacgaac atgtcgctgg tcgccaagaa gtggagcgca
     1321 ccgggcaagc cggcggcgca gacggtgcag gtctgggcgc tcgacatgga ctcggcggat
     1381 ccgctgagcg gggtcgaggt cagcctggtc cgcaagagcg gcaaggtggt ggcgacgtgc
     1441 acgaccagcg gcggcgacgg ctgcacgctg gcggcgcgga ccgacgacga tcccgacgac
     1501 gcggtgccgt tcgcggtgat cgcgaagaag ggcgacgacc tgacctacat ccgctaccag
     1561 gacctgcgcg ccgacgtcgc ggagtcgagc acgtcgggcg tcgaggtggt cgccgacgcg
     1621 ccgtaccacg cggcgatcta tcccgatcgc gacgtgttcc gtccgggc
//
LOCUS       DASVOR010002510         1534 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_174013,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002510 DASVOR010000000
VERSION     DASVOR010002510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174013"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1534)
                     /locus_tag="VF516_46105"
     CDS             complement(<1..>1534)
                     /locus_tag="VF516_46105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115211.1"
                     /translation="AQPVLLVLEDLHWGDFGTVRFVDTALRDRRDRPWMVLALARPEV
                     LEVFPRLWAERQNVQEIRLQELGRKAGERLVRQVLGDSVGPETLERLVRHAGGNAFYL
                     EELIRAAAEGKDHALPDTVLAMVETRLARLSVEARRVLRAASVFGEVCWEGGVAALLD
                     GAMTAAAVAEWLERLAEQEVLAVRPHSRVPGERELAFRHALLREGTYATLTEDDKRLG
                     HRLAGTWLEHHGESDPMVLAGHFERGGNGERAAEFYLRAAEQAHPVLLDREATLARAA
                     LGLACAPTPPLRIALLGIRCEAAAAVLHLIRDVLADVEEVLRTAPRGTKPWAQAMLAY
                     SETILFAGRTDDLRALVTLLRDLDPVPEAVTTTALVLLTTICMLDAFGFVADATALEE
                     RLAFIRSRAEREPMVQLWWHTAIGMRAGYAHDDPWRGLQDSDAIQAIHAEARSEVMVL
                     NMQLFRALNLWYLGAFEAAEQLLRSIAAADEAIGPASSLRRLTLAWLLADRGALDPAR
                     AVA"
BASE COUNT          217 a          568 c          562 g          187 t
ORIGIN      
        1 cgccacggcg cgcgccgggt cgagcgcgcc gcggtcggcg agcagccacg ccagggtgag
       61 ccggcgcagc gagctggccg ggccgatcgc ctcgtcggcc gcggcgatgc tgcgcagcaa
      121 ctgctccgct gcctcgaacg cgcccaggta ccacaggttc agggcgcgga acagctgcat
      181 gttcagaacc atcacctcgc tgcgggcctc ggcgtggatc gcctggatcg catcgctgtc
      241 ctgcaggccg cgccacgggt catcgtgagc gtagccggcg cgcatcccga tcgcggtgtg
      301 ccaccagagc tggaccatcg gctcgcgctc ggcccgcgac cggatgaacg ccaggcgctc
      361 ctccagcgcg gtcgcatccg cgacgaagcc gaacgcatcg agcatgcaga tcgtggtgag
      421 caggaccagc gccgtggtcg tgaccgcctc gggcaccggg tcgaggtcgc gcagcagcgt
      481 gacgagcgcg cggaggtcgt cggtccggcc ggcgaacagg atggtctcgg agtacgccag
      541 catcgcctgt gcccatggct tcgtcccgcg cggcgccgtg cgcagcacct cctcgacatc
      601 ggccaggaca tcgcggatca ggtgcaggac agcggcggca gcctcgcagc gtatgccgag
      661 caaggcgatc cggagcggag gcgtcggcgc gcacgccaga ccgagcgcag cacgcgccag
      721 cgtggcctcg cgatcgagca gcacgggatg ggcctgctcg gcggcgcgca ggtagaactc
      781 cgcggcgcgc tcaccgttgc cgccgcgctc gaagtgaccg gcgagcacca tgggatcgct
      841 ctcaccgtgg tgctccagcc acgtgccggc gagccggtgg ccgagccgct tgtcgtcctc
      901 ggtcaaggtc gcgtaggtgc cctcgcggag cagggcgtga cggaacgcga gctcgcgctc
      961 gccggggacc cggctgtgcg gccgcaccgc cagcacttcc tgctcggcca ggcgctccag
     1021 ccactcggcg accgccgccg cggtcatcgc gccgtcgagc agggccgcga cgccgccctc
     1081 ccagcacacc tcgccgaaca cgctggcggc gcgcagcacg cggcgggcct cgaccgagag
     1141 ccgggcgagc cgggtctcga ccatcgccag cacggtgtcg ggcagcgcgt ggtccttgcc
     1201 ctcggccgcg gcgcggatca gctcctcgag gtagaacgcg ttgccgccgg cgtgcctcac
     1261 caggcgctcc agggtctcgg gaccgacgct gtcgccgagc acctggcgga ccaggcgctc
     1321 gccggccttg cggcccagct cctggagccg gatctcctgg acgttctggc gctcggccca
     1381 cagcctgggg aacacctcga gcacctcggg gcgggcgagc gcgagcacca tccacggccg
     1441 gtcgcggcga tcgcgcagcg cggtgtcgac gaaccggacg gtgccgaagt cgccccagtg
     1501 caggtcctcg agcacgagca ggacgggctg ggcg
//
LOCUS       DASVOR010002511         1845 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_262378,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002511 DASVOR010000000
VERSION     DASVOR010002511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1845
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262378"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1719)
                     /locus_tag="VF516_46110"
     CDS             complement(<1..1719)
                     /locus_tag="VF516_46110"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEX8115212.1"
                     /translation="MSHLRYAIRLDAVRHLAEIELRFPVDTDTVELALPAWSPGSYLI
                     RDYARFVRDLAVTGDDGAPRQAAKRDKSTWTVDVAGARELRVRYAVYGHDLSVRTNHI
                     DAEHAFLHGPATFVHPTHLRAAPVEVELAVPDGWIVTTAMAWDPSPPYRLTAADIDEL
                     YDHPIHAGPTRTVMVAAAVPVKLAIWGARAPGGTFDEARLAADLGAVVNDHVARFGAA
                     PFPHYTFLVMLAHDAYGGLEHRASSVNLYHPYFAASRKHYEGLLELLSHELFHVWNGK
                     RIAPRALLRPDYARESYTRCLWLMEGLTSHYDRYALRTSNRITARSFLDKVLDDWARL
                     MATPGRRRHSLEGASFDAWIKLYRPDESNLNTTVSYYLKGGLTMLALDLQIRRRTEGA
                     RSLDDVVRLLWQRYGAPGEPHPEQLQPVFEEAAGLSLADVFDRQIRGTADPELAEELR
                     HVGLELRATTDPAQLADAGRPVWIGATLTGGKITGVHDDSPAQAAGLSPGDEIIALDG
                     FRVTGEAEVRSLAGALAPGDRIELAVFRRARLLRLPLTLGAALPTRYEIAGLPDAGAA
                     AARYHVW"
BASE COUNT          232 a          636 c          718 g          259 t
ORIGIN      
        1 ccagacgtgg tagcgcgccg cggccgcgcc ggcgtccggt aggcccgcga tctcgtagcg
       61 ggtcggcagc gccgcaccga gcgtgagcgg cagccggagc aggcgcgcgc ggcggaacac
      121 cgcgagctcg atccggtcgc ccggggcgag cgcgccggcg agcgagcgca cctcggcctc
      181 gccggtcacc cggaagccgt cgagcgcgat gatctcgtcg cccggcgaca gccccgcggc
      241 ctgggccggc gagtcgtcgt gaacgccggt gatcttgccg ccggtcagcg tcgcgccgat
      301 ccacaccggg cggccggcgt cggcgagctg cgccggatcg gtggtcgcgc gcagctcgag
      361 gccgacgtgg cgcagctcct cggcgagctc ggggtcggcg gtgccgcgga tctggcggtc
      421 gaacacgtcg gcgagcgaca gcccggccgc ctcctcgaac accggctgga gctgctcggg
      481 gtgcggctcg cccggcgcgc cgtagcgctg ccacagcagg cgcaccacgt cgtcgaggct
      541 gcgcgcgccc tcggtgcggc ggcggatctg gagatcgagc gcgagcatgg tcagcccgcc
      601 cttgaggtag tagctgaccg tggtgttgag gttggactcg tcgggcctgt acagcttgat
      661 ccaggcgtcg aacgacgcgc cctcgaggct gtggcggcgg cggcccgggg tcgccatcag
      721 ccgggcccag tcgtcgagca ccttgtcgag gaagctgcgc gcggtgatcc ggttgctggt
      781 gcgcagcgcg tagcggtcgt aatggctggt caggccctcc atcagccaca ggcaccgcgt
      841 gtagctctcg cgcgcgtagt cgggccggag cagcgcgcgc ggcgcgatcc gctttccatt
      901 ccatacgtgg aatagctcgt gcgacagcag ctcgagcagc ccctcgtagt gcttgcgcga
      961 cgcggcgaag tacgggtggt agaggttgac gctcgacgcg cggtgctcga ggccgccgta
     1021 ggcgtcgtgc gccagcatca cgaggaacgt gtagtgcggg aacggcgcgg cgccgaaccg
     1081 cgcgacgtga tcgttgacga ccgcgccgag gtcggcggcg agccgggcct cgtcgaacgt
     1141 gccgcccggg gcgcgcgcgc cccagatcgc gagcttgacc ggcaccgccg ccgcgaccat
     1201 gaccgtgcgg gtcggcccgg cgtggatcgg atggtcgtag agctcgtcga tgtcggccgc
     1261 ggtcagccgg tacggcgggc tcggatccca ggccatcgcg gtggtcacga tccagccgtc
     1321 ggggaccgcg agctcgacct cgaccggcgc ggcgcgcagg tgggtcgggt ggacgaacgt
     1381 cgccgggccg tgcaggaacg cgtgctcggc gtcgatgtgg ttggtgcgca cgctgaggtc
     1441 gtggccgtac accgcgtagc ggacgcgcag ctcgcgggcg ccggcgacgt cgaccgtcca
     1501 ggtcgacttg tcgcgcttgg cggcctggcg cggcgccccg tcgtcgccgg tcaccgcgag
     1561 gtcgcgcacg aaccgcgcgt agtcgcggat caggtagctc cccggcgacc acgccggcag
     1621 ggcgagctcc accgtgtcgg tatctaccgg gaaccgcagc tcgatctccg ccaggtggcg
     1681 gaccgcatcg agccggatgg cgtagcggag atgcgacatg caccaggtgc tagcacagct
     1741 gctatcctcg atgcgtgtcg cggtcgatgt gcgccgtgct cgtcgcgtgc tcgctcgcgt
     1801 cgctggggcc gtccccggct gcggtcgctg gtcccagcgg ccggg
//
LOCUS       DASVOR010002512         1918 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_174025,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002512 DASVOR010000000
VERSION     DASVOR010002512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_174025"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(114..677)
                     /gene="efp"
                     /locus_tag="VF516_46115"
     CDS             complement(114..677)
                     /gene="efp"
                     /locus_tag="VF516_46115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEX8115213.1"
                     /translation="MYDTSDIRKGLKVLMDGNPYTVIEFQFVKPGKGAAFTRTKFKNL
                     LTGAVVERNIRSGEKLEPANVETRDMQYLYKEGGDLVFMDQTNYEQVTISAELIGPAH
                     DLMKDNLPCTVVFFNERPVDVTLPTFVFLEVTASEPGVRGDTSGNVTKPATVETGATV
                     NVPLFIRIGDTVKIDTRTHEYVERVNK"
     gene            804..1337
                     /locus_tag="VF516_46120"
     CDS             804..1337
                     /locus_tag="VF516_46120"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8115214.1"
                     /translation="MDAAAHYRHLLGLGRDEFLAAAAPAVLLRYRAQAPDPGPGPGTL
                     TMTIDEDFGEAVDETMPHGKFTPEPVEMELYPLAKKPGASFPDRITIGRTANNDVVIA
                     DPSVSRLHAYVRQADGWVVADAGSKNGSWLDAAPLEPRRETALPSRSVLRLGDVLLTF
                     YHSEELFDLLGGDHGRR"
     gene            1324..>1918
                     /locus_tag="VF516_46125"
     CDS             1324..>1918
                     /locus_tag="VF516_46125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115215.1"
                     /translation="MAVGEWDQRQLCPDGACIGVIGPDGTCKVCGRAAPNWGDERNRG
                     LIDPPDEDEDDEDEEGDEDLAADGDDEDDEEEDDEDDEDDDDDDSDDEDDDDDDSDDE
                     EADQVSKPGVATARRGEWAARQLCPDGGCIGVIGPDGRCNMCGRSASKDPAAAAAPPA
                     AQPASSSSKPDPDVLEVTTDPVNIPDILPVLTVRTHVV"
BASE COUNT          305 a          656 c          669 g          288 t
ORIGIN      
        1 caggtcgccg ggccgccagc cggcgcgtgt gcgcacgcgc gcatcgccgc gctcgccgcg
       61 cacgatcgcg agctcgccgt cgaccgcgag cacgcggccg cggaccgctc gggtcacttg
      121 ttgacgcgct cgacgtactc gtgcgtgcgc gtgtcgatct tgaccgtgtc accgatccgg
      181 atgaacagcg ggacgttgac ggtggccccg gtctcgacgg tcgcgggctt ggtcacgttg
      241 ccgctggtgt caccgcgcac gccgggctcg gaggccgtga cctcgaggaa cacgaacgtg
      301 ggcagggtca cgtcgaccgg gcgctcgttg aagaacacca ccgtgcacgg caggttgtcc
      361 ttcatcagat cgtgcgccgg gccgatcagc tcggcgctga tcgtgacctg ctcgtagttg
      421 gtctggtcca tgaagaccag gtctccgccc tccttgtaga ggtactgcat gtcgcgggtc
      481 tcgacgttgg ccggctccag cttctcgccc gaccggatgt tgcgttcgac gacagcgccg
      541 gtgagcaggt tcttgaactt ggtgcgggtg aacgcggcgc ccttgcccgg cttcacgaac
      601 tggaactcga tgacggtgta cgggttcccg tccatcagca ccttgagacc cttgcgaata
      661 tcggacgtgt cgtacatgac ccgggacgtt taccttgcgc atagcgggcc tgtcaaaccg
      721 cgaattctgc gccgatccga cgacaccggc gatgcccgcc gcgggagctt gccgcgccgg
      781 cccctgtggg cgataagcac cgcgtggacg ccgccgcgca ctaccgccac ctgctcgggc
      841 tcgggcggga cgagttcctg gccgcggccg cgcccgccgt cctgctgcgc tatcgtgccc
      901 aggcgcccga tcccggcccg gggccgggca ccctgacgat gacgatcgat gaagacttcg
      961 gcgaagcggt ggacgagacc atgccgcatg gcaaattcac cccggagcct gtcgagatgg
     1021 agctgtatcc gctcgcgaag aagcccggcg cctcgttccc ggatcgcatc acgatcggtc
     1081 ggaccgccaa caacgacgtg gtgatcgccg atccgtcggt gtcgcggctg cacgcctacg
     1141 tccgtcaggc cgatggctgg gtcgtcgccg acgccggctc caagaacggc agctggctcg
     1201 acgcggcgcc gctcgagccc cgccgggaga ccgcgctgcc gtcacgctcg gtgctccggc
     1261 tgggtgacgt gctgctcacc ttctaccact ccgaggagct gttcgacctg ctgggaggcg
     1321 accatggccg tcggtgaatg ggatcaacgc cagctgtgcc ccgacggcgc ctgcatcggc
     1381 gtgatcgggc ctgacgggac gtgcaaggtg tgcggccgcg ccgctccgaa ctggggcgac
     1441 gagcgcaacc gcgggctgat cgatccgccg gacgaggatg aggacgacga ggatgaggag
     1501 ggcgacgagg acctcgcggc cgacggcgac gatgaggacg atgaggagga ggacgacgag
     1561 gacgacgagg acgacgacga cgacgacagc gacgacgagg acgacgacga cgacgacagc
     1621 gacgacgagg aggccgacca ggtctccaag ccgggagtcg cgaccgcgcg gcgcggcgag
     1681 tgggcagcgc gccagctgtg ccccgacggc ggctgcatcg gcgtgatcgg ccccgatggg
     1741 cgatgcaaca tgtgcgggcg ctctgcctcg aaggatcccg cggcagccgc cgcccctccc
     1801 gccgcacagc cggcctcgtc gtccagcaag ccggatcccg acgtactgga agtcaccacc
     1861 gatccggtca acatcccgga catcctcccg gtcctcacgg tgcgaacgca tgtcgtgt
//
LOCUS       DASVOR010002513         4761 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58046,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002513 DASVOR010000000
VERSION     DASVOR010002513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58046"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1469
                     /locus_tag="VF516_46130"
     CDS             <1..1469
                     /locus_tag="VF516_46130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEX8115216.1"
                     /translation="DRGHATTWEPPHAVTVDRERAANSAWYELFPRSASPDPQRPGTL
                     ADVIARLDYVAELGFDVLYLPPIHPIGRAFRKGRDNAPTAGPDDPGSPWAIGGPEGGH
                     TSIHPALGTLADFDHLVAAARDRGIEIALDIAFQASPDHPWVREHPAWFKARPDGTIQ
                     YAENPPKKYQDVYPFDFDTADWQALWRALRDVFVFWCERGVRMFRVDNPHTKPIRFWQ
                     WCLADVQARYPDAIFLSEAFTRPKLMYALAKIGFSQSYTYFTWRTTRAELEAYATEIA
                     RTEVAEFFRPSLWPTTPDIFPEHLVHGGRAAFAIRLVLAGTLSANYGIYGPSFELGER
                     EPRPGAEELARNEKYEIRGWDLDRPDSLRHVIARLNRIRRAHPALHGNRSLRFHPTDN
                     DLVICYSKRTPDHRDVVLVVVNLDPHHVHRAHLTLDLAALGLAADQGFQVHDLLGDAR
                     YTWRGPRNFVELVPDTSPAHIFEVKRFVRSENQFEYFL"
     gene            1466..>4761
                     /gene="treS"
                     /locus_tag="VF516_46135"
     CDS             1466..>4761
                     /gene="treS"
                     /locus_tag="VF516_46135"
                     /EC_number="5.4.99.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012829192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047471 - maltose
                     alpha-D-glucosyltransferase activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose alpha-D-glucosyltransferase"
                     /protein_id="HEX8115217.1"
                     /translation="MTDIALSDDPLWFKDAIVYEIHIRAFQDSTNDGVGDITGLIERL
                     DYLCDLGITAIWLLPFYPSPLKDAGYDIADYTSVHDHYGTREDFARLLHEAHRRGIRV
                     ITELVLNHTSADHPWFQAARRAPAGSPERDFYVWSDTPSRYEDTRIIFKDYETSNWAW
                     DPVAKAYYWHRFYVHQPDLNFDNPAVHDALFRVIDFWLEMGVDGLRLDAVPYLYEREG
                     TSCENLEETHAFLKRLRAHIDGKFKNRMLLAEANQWPADAAAYFGAGDECHMNFHFPL
                     MPRMFMALETEDSFPIVNILKRTPRIPESCQWATFLRNHDELTLEMVTDEDRDTMYRG
                     YAAERAARINLGIRRRLAPLLGVRRKIELMTALLLSLPGTPVLYYGDEIGMGDNIYLG
                     DRDGVRTPMQWSSDRNGGFSRANPQKLYLPTIIDPEYHYEAINVEAQQGNPSSLLWWM
                     KRLLAKRKEHRLFGRGSIEFIPGDNPRVLAFIREYEGETVLVVANLSRSVQCTRLKLE
                     RFHAMVPIELFGRTRFPEILDVPYFLSLGPYDFYWLALERPQSAETAAERPVLTAKTS
                     WSALLEPSRRTQLARTLLGYAIQRRWFRGKARTRKQVSISDVLTFEADARHAIVLLHV
                     EYDHGRPETYVVPIAFSEDTDGIDPAHTGLAHTGLAHTSGLVIARVTLTDVAERGAIH
                     GVLYDAVCSEAFCAALLGAMVTGGGAAGRVGRLTGDALPALRDAIAAGTPLAARAMAV
                     DQSNSSVLYGDRFLLKLFRVIEDGPSSELEFGRLFAGRARDYRGVPRLAGVLEYRQSS
                     HEPGTLGTLFEYVANQGDAWRLAMDEIDRYYDRVLTDERHPEAPVIERGSLVERARAP
                     LSDRVVDMLGPFLDHIRLLGRRTAELHLQLAAATDDPMFAPEPYDILHQQSMYGTVVA
                     NAARTFDLLRSRLAALHPEQRALAEVVLAREPELDRVLAQITRRRIDVIRTRTHGDYH
                     LGQVLWTGDDFVIIDFEGEPGRPLSQRRFKHNPLRDVAGMLESLRYAGVAELRSGRHR
                     PEDVARLEPWARAWSGWMSAAFLGGYREAARGSRILPRSDADLGFLLEFFVLEKCVYE
                     IGYELNNRPD"
BASE COUNT          720 a         1761 c         1575 g          705 t
ORIGIN      
        1 ccgatcgcgg gcacgcgacg acgtgggagc cgccgcacgc cgtgaccgtg gaccgtgagc
       61 gggcggcgaa ttccgcctgg tacgagctgt tcccgcggtc ggcctcgccc gatccgcagc
      121 gaccgggaac gctggccgac gtcatcgcac gtctggacta cgtcgccgag ctcggcttcg
      181 acgtgctcta cctgccgccg atccatccga tcggccgcgc gttccgcaag ggccgcgaca
      241 acgcgccgac cgccgggccc gacgatccgg gcagcccgtg ggcgatcggc ggacccgagg
      301 gcggtcacac cagcatccac cccgcgctcg gcacgctcgc cgacttcgat cacctggtcg
      361 cggcggcgcg cgatcgcggc atcgagatcg cgctcgacat cgcgttccag gcctcgccgg
      421 atcacccgtg ggtccgcgag cacccggcgt ggttcaaggc ccggcccgac ggcacgatcc
      481 agtacgccga gaacccgccc aagaagtacc aggacgtcta cccgttcgac ttcgacacgg
      541 ccgactggca ggcgctgtgg cgcgcgctgc gcgacgtgtt cgtgttctgg tgcgagcgcg
      601 gcgtgcggat gttccgggtc gacaacccgc acaccaagcc gatccggttc tggcagtggt
      661 gcctcgccga cgtccaggcg cgctaccccg acgcgatctt cctgtccgag gcgttcaccc
      721 ggcccaagct gatgtacgcg ctggccaaga tcggattcag ccagtcctat acctacttca
      781 cctggcgcac gaccagggcc gagctcgagg cctacgcgac cgagatcgcg cgcaccgagg
      841 tcgccgagtt cttccggccg agcctgtggc cgaccacgcc ggacatcttc cccgagcacc
      901 tggtccacgg cggccgcgcc gcgttcgcga tccggctcgt gctcgcgggc acgctgtccg
      961 cgaactacgg gatctacggc ccgtcgttcg agctcggcga gcgcgagccc cggccgggcg
     1021 ccgaggagct ggcgcgcaac gagaagtacg agatccgcgg ctgggacctc gatcgcccgg
     1081 actcgctgcg ccacgtgatc gcgcggctca accggatccg ccgcgcccac cccgcgctgc
     1141 acggcaatcg cagcctgcgg tttcacccga ccgacaacga tctggtgatc tgctactcca
     1201 agcgaacacc ggatcaccgc gacgtcgtgc tggtcgtggt caacctcgat ccgcaccacg
     1261 tccatcgcgc gcacctgacc ctggatctcg ccgcgctcgg gctcgccgcc gaccaggggt
     1321 tccaggtcca cgacctgctc ggcgacgcgc gctacacctg gcgcggcccg cgcaacttcg
     1381 tcgagctcgt tccggacacg agcccggccc acatcttcga ggtcaagagg ttcgtgcgca
     1441 gcgagaacca attcgagtac ttcctatgac cgatatcgca ctgtccgatg atccgctgtg
     1501 gttcaaggac gcgatcgtct acgagatcca catccgcgca ttccaggact cgaccaacga
     1561 cggggtcggc gacatcaccg gcctgatcga gcgcctggac tacctgtgcg atctcgggat
     1621 cacggcgatc tggctgttgc cgttctaccc gtcgccgctc aaggacgccg gctacgacat
     1681 cgccgactac acgagcgttc acgaccacta cggcacgcgc gaggacttcg cccggctgct
     1741 ccacgaggcc caccgccgcg gcatccgggt gatcaccgag ctcgtgctca accacacctc
     1801 ggcggaccat ccctggttcc aggccgcgcg gcgcgccccg gccggcagcc cggagcgcga
     1861 cttctacgtg tggagcgaca ccccgtcgcg ctacgaggac acccggatca tcttcaagga
     1921 ctacgagacc tcgaactggg cgtgggatcc ggtcgccaag gcctactact ggcaccggtt
     1981 ctacgtccac cagcccgatc tcaacttcga caacccggcg gttcacgacg cgctgttccg
     2041 cgtgatcgac ttctggctcg agatgggggt cgacgggctg cggctcgacg ccgttcccta
     2101 cctgtacgag cgcgagggca ccagctgcga gaacctcgag gagacccacg cgttcctcaa
     2161 gcggctgcgc gcccacatcg acggcaagtt caagaaccgc atgctgctcg ccgaggccaa
     2221 ccagtggccg gcggatgcgg cggcgtactt cggcgccggc gacgagtgcc acatgaactt
     2281 ccacttcccg ttgatgccgc ggatgttcat ggcgctcgag accgaggact cgttcccgat
     2341 cgtcaacatc ctcaagcgca cgccgcggat cccggagtcg tgccagtggg cgacgttcct
     2401 gcgcaaccac gacgagctga ccctggagat ggtgaccgac gaggaccgcg acacgatgta
     2461 ccgcggctac gccgccgagc gcgccgcccg gatcaacctc gggatccggc gccggctcgc
     2521 gccgctgctc ggcgtgcgcc gcaagatcga gctgatgacc gcgctgctgc tgtcgctgcc
     2581 cggcacgccg gtgctgtact acggcgacga gatcgggatg ggcgacaaca tctacctggg
     2641 cgaccgcgat ggcgtgcgca cgccgatgca gtggagctcg gaccgcaacg gcgggttctc
     2701 gcgcgccaac ccgcagaagc tgtacctgcc gacgatcatc gatcccgagt accactacga
     2761 ggcgatcaac gtcgaggctc agcagggtaa cccgtcgtcg ctgctgtggt ggatgaagcg
     2821 gctgctcgcc aagcgcaagg agcaccggct gttcggccgg ggctcgatcg agttcatccc
     2881 gggcgacaac ccgcgcgtgc tggcgttcat ccgcgagtac gagggcgaga ccgtgctggt
     2941 cgtcgccaac ctgtcgcgct cggtgcagtg cacgcggctc aagctcgagc ggttccacgc
     3001 catggtgccg atcgagctgt tcgggcggac ccggttcccc gagatcctcg acgtgccgta
     3061 cttcctgtcg ctcgggccct acgacttcta ctggctggcg ctcgagcggc cgcagtccgc
     3121 cgagaccgcc gcggagcgcc cggtgctgac cgcgaagacc agctggagcg cgctgctcga
     3181 gccgtcgcgc cgcacccagc tcgcgcgcac cctgctcggc tacgcgatcc agcgccggtg
     3241 gttccgcggc aaggcgcgca cccgcaagca ggtgtcgatc agcgacgtcc tgacgttcga
     3301 ggccgacgcg cgccacgcga tcgtgctgct ccacgtcgag tacgaccacg gccggcccga
     3361 gacctacgtg gtgccgatcg cgttctccga ggacaccgac ggcatcgatc ctgcacacac
     3421 cggcctggca cacaccggcc tggcgcacac ctcgggcctg gtgatcgcgc gggtcaccct
     3481 gaccgacgtc gccgagcgcg gcgcgatcca cggcgtgctg tacgacgcgg tgtgctccga
     3541 ggcgttctgc gccgcgctgc tcggcgcgat ggtcaccggg ggcggggccg ccgggcgggt
     3601 cggccggctc accggcgacg cgctgcccgc gctgcgcgac gcgatcgccg ccgggacacc
     3661 gctcgcggcg cgcgcgatgg cggtcgacca gagcaacagc agcgtgctgt acggcgaccg
     3721 cttcctgctc aagctgttcc gcgtgatcga ggacggcccg agcagcgagc tcgagttcgg
     3781 ccggttgttc gccgggcgcg cgcgcgacta ccgcggcgtc ccccggctcg ccggcgtgct
     3841 ggagtaccgc cagtccagcc acgagccggg cacgctgggc acgctgttcg agtacgtggc
     3901 caaccagggc gacgcgtggc ggctcgcgat ggacgagatc gatcgctact acgaccgcgt
     3961 gctgaccgac gagcgccacc ccgaggcgcc ggtgatcgag cgcggctcgc tggtcgagcg
     4021 cgcgcgcgcg ccgctgtccg atcgcgtggt cgacatgctc ggcccgttcc tcgatcacat
     4081 ccggctgctc ggccgccgca ccgccgagct ccacctccag ctcgccgccg cgaccgacga
     4141 tccgatgttc gcgcccgagc cgtacgacat cctgcaccag cagtcgatgt acggcaccgt
     4201 ggtcgccaac gccgcgcgca cgttcgacct gctgcgcagc cggctggccg cgctgcaccc
     4261 cgagcagcgc gcgctcgccg aggtcgtgct ggcgcgcgag cccgagctcg atcgcgtgct
     4321 cgcgcagatc acgcgccggc ggatcgacgt gatccgcacc cggacccacg gtgactacca
     4381 cctcggccag gtgctgtgga ccggcgacga cttcgtgatc atcgacttcg agggcgagcc
     4441 cggccgcccg ctgtcgcagc gccggttcaa gcacaacccg ctgcgcgacg tcgccggcat
     4501 gctcgagtcg ctgcgctatg ccggtgtcgc cgagctgcgc agcggccggc accgccccga
     4561 ggacgtcgcc cggctcgagc cgtgggcgcg cgcgtggtcg ggctggatgt ccgcggcgtt
     4621 cctcggcggt taccgcgagg ccgcgcgggg ctcgcggatc ctgccgcgca gcgacgcgga
     4681 cctgggcttc ttgctggagt tcttcgtgct ggagaaatgc gtgtacgaga tcggctacga
     4741 gctcaacaac cgtcccgact g
//
LOCUS       DASVOR010002514         2395 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_378804,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002514 DASVOR010000000
VERSION     DASVOR010002514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378804"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="VF516_46140"
     CDS             complement(<1..405)
                     /locus_tag="VF516_46140"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8115218.1"
                     /translation="MRIVLVEDNLQLATTISDGLSEDGYTVDVVATAAGAIERGLRRD
                     LDVMVLDLGLPDRDGLEVLRELRGVRINVPILVLTARDAVEARVAALDAGADDYLVKP
                     FAFAELVARIAALARRAAGPRWAPATDMPLTMR"
     gene            complement(523..1140)
                     /locus_tag="VF516_46145"
     CDS             complement(523..1140)
                     /locus_tag="VF516_46145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115219.1"
                     /translation="MKPIKSFLVLAILLAPALASAQGYYGRPYPGSTVPGGFHNRAGH
                     IIFGVGGGLGFMHDDGSAITTCDNCDFKPYAGELDLHIGGMLTPRFGLMFEAQGNFQT
                     IHSDFFNGDTTITQGAGMLAGQFWLLPQLWIKGGLGFSNLHVDDTFVTQDIGNGLAVM
                     GAIGVELFSARNFAFEVQGRLIEGTYNSGNDHVTSGTIGLGLDWF"
     gene            complement(1358..>2395)
                     /locus_tag="VF516_46150"
     CDS             complement(1358..>2395)
                     /locus_tag="VF516_46150"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEX8115220.1"
                     /translation="DQPVEEVATGRTPTPTPPPAEPVPEPAPGPRTISARYLRSGRWV
                     PLRVGSLSLKGAALMAVALPRVNDRVDVALAYAQHRVLVRGAVQKVSTMREAETSGAT
                     MFSVSFELDDGSRRQLTELLTAARAANVTIKPAPPRGTRRYPVEWPVCLGTSRGAVKA
                     EAFDVSADGMFVKPIHPLSLEAHVTFSAVLDDNQPPVSGHARVVRNISEAEARDAGLW
                     SGYGLVILEMADPERERWSRFLRRVATRASKRVLIAASAARLTELESGLVAGGYATSG
                     GTEPGAIAQLVSAEARPFDAALIDAAWLLAEGSTLWVQSVFTERSIPYVTVHGDARRA
                     RQAIDRLLAVV"
BASE COUNT          359 a          881 c          811 g          344 t
ORIGIN      
        1 tcgcatcgtc agcggcatgt cggtcgccgg cgcccagcgc ggcccggcgg cgcgccgcgc
       61 gagcgctgcg atccgcgcga ccagctcggc gaacgcgaac ggcttgacca ggtagtcgtc
      121 ggcgccggcg tcgagcgccg cgacccgggc ctcgaccgcg tcgcgcgcgg tcagcaccag
      181 gatcggcacg ttgatccgca cgccgcgcag ctcgcgcagc acctcgaggc cgtcgcggtc
      241 cggcagcccg agatcgagca ccatgacgtc gagatcgcgg cgcaggccgc gctcgatcgc
      301 gcccgcggcg gtcgcgacga cgtcgaccgt gtagccgtcc tccgacaggc cgtcggagat
      361 cgtggttgcg agttgcaggt tgtcttcgac caggacgatt ctcatggcga gccccggcgg
      421 gtggcggtca gggtcatgtc ccaaggtttc ccgcaaaccc gcgggtcggg acattatgac
      481 tcgctaaatt tcgccgccgc tcgccgggga ccccggccgg gttcagaacc agtccaggcc
      541 gagcccgatc gtccccgagg tcacgtggtc gttgccgctg ttgtacgtac cctcgatcag
      601 ccggccctgg acctcgaacg cgaagttgcg cgccgagaac agctcgacgc cgatcgcgcc
      661 catcaccgcc aggccgttgc cgatgtcctg ggtgacgaac gtgtcgtcga cgtgcaggtt
      721 cgagaacccg aggccgccct tgatccacag ctgcggcagg agccagaact gcccggcgag
      781 catcccggcg ccctgcgtga tcgtcgtgtc gccgttgaag aagtcggagt ggatggtctg
      841 gaagttaccc tgggcctcga acatcagccc gaaccgcggc gtcagcatgc cgccgatgtg
      901 caggtcgagc tcaccggcgt acggcttgaa gtcgcagttg tcgcacgtgg tgatggcgct
      961 gccgtcgtcg tgcatgaacc cgaggccgcc gccgaccccg aagatgatgt ggcctgcgcg
     1021 gttatggaat ccgccgggga ctgtcgaccc cggatagggc cggccgtagt agccctgcgc
     1081 cgacgccagc gccggggcca acaggatcgc gagaacgagg aaggacttga taggcttcat
     1141 acgctgacct tggaaacggc ggaaggccgt gacccgaagc cttcgcaagt ccggggccaa
     1201 gctgcaagcg gtctgcgtgg ttctctggag cgacgcaatt ctcgggggtt cgcgcgtcac
     1261 tccaggatgc ttgtgcgcat tcctgcgcac gatcaccacc tggcgcgtga tgtctgaacg
     1321 caacgttgtg aaccatcgtc cactgatcgc ccgcgaatca caccacagcg agcaagcggt
     1381 cgatcgcctg gcgcgcccgc ctggcgtcgc cgtgcaccgt cacgtacggg atgctgcgct
     1441 cggtgaacac cgactgcacc cacagcgtcg aaccctccgc gagcagccat gccgcgtcga
     1501 tcagcgccgc gtcgaacggg cgcgcctccg cactcaccag ctgcgcgatt gcgcccggct
     1561 cggtgccgcc cgacgtcgcg tagccgccgg cgaccagccc gctctcgagc tcggtgagcc
     1621 gcgccgccga cgccgcgatc agcacccgct tgctcgcccg cgtggcaacc cgcctcagga
     1681 acctcgacca ccgctcccgc tccggatcgg ccatctccag gatcaccagc ccgtagcccg
     1741 accacagccc ggcgtcccgg gcctccgcct cgctgatgtt gcggaccacc cgcgcgtgcc
     1801 ccgacaccgg cggctggttg tcgtcgagca ccgccgagaa cgtgacgtga gcctcgagcg
     1861 agagcgggtg gatcggcttg acgaacatcc cgtcggccga cacgtcgaac gcctcggcct
     1921 tcaccgcgcc ccgcgacgtg cccaggcaca ccggccactc gaccgggtag cgccgcgtgc
     1981 cgcgcggcgg cgccggcttg atcgtgacgt tcgcggcgcg cgcggcggtc agcagctcgg
     2041 tgagctggcg gcgcgagccg tcgtcgagct cgaagctgac gctgaacatc gtcgcgccgc
     2101 tggtctcggc ctcccgcatc gtcgagacct tctggaccgc gccgcgcacc agcacacgat
     2161 gctgcgcgta cgccagcgcg acgtcgaccc ggtcgttgac ccgcggcagc gccaccgcca
     2221 tcagcgcggc gcccttgagc gacagcgagc cgacccgcag cggcacccag cgcccgctgc
     2281 gcaggtagcg cgccgagatc gtccgcggcc cgggcgcggg ctcgggtacg ggctccgcgg
     2341 gcgggggcgt cggcgtcggc gtgcgaccgg tcgcgacctc ctcgaccggc tgatc
//
LOCUS       DASVOR010002515         4443 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58063,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002515 DASVOR010000000
VERSION     DASVOR010002515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4443
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58063"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1348)
                     /locus_tag="VF516_46155"
     CDS             complement(23..1348)
                     /locus_tag="VF516_46155"
                     /inference="COORDINATES: protein motif:HMM:NF037663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="styrene monooxygenase/indole monooxygenase
                     family protein"
                     /protein_id="HEX8115221.1"
                     /translation="MGSGLSGLLAAHALRKAGHEITLYTDRSATQWLDESRPTGAAAR
                     FELALGYERELGLAHWEAAGAKCNGIHLTFCPSPGNRMVTLNGVLASTYFQAVDLRLQ
                     SHRWMNDLEAAGGRIVIETIDIDRLDAIAAQHDLVLVASGRGALAELFPRNARRSVYT
                     AAQRKLAMVIVTGADGACDGPPFEAVKIHLLAQHGEVIWMPYHHKDHGPTLVMLFEAR
                     PDSAMDRFDGCRSGAEVVAMAKTVLAELIPWDHQWARNMELADRNGWLMGSVKPTIRE
                     PFGRLPSGRVVMPIGDTAMSVDPIGGQGANLGAKLVRHLAQAIAADPGVTFDAAWMVR
                     TFDAFWADHGSPTVTFNNALLEPLTPAGRLMLISQHGSTGEGDSPKQRIANAIVENFA
                     DPRRFTDAFIDRRAAQRRIAELTGHSWPRELLGGALRVGQGQLRRVFEA"
     gene            complement(1940..2431)
                     /locus_tag="VF516_46160"
     CDS             complement(1940..2431)
                     /locus_tag="VF516_46160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115222.1"
                     /translation="MSDVDLTEAVRVLRDEVLDTVHHGDRDPPGAEVFDALLHALAIG
                     GESVPGLDLTLHDAVARRLAWGDSEEAVLADAELVFDRLMVSVERAFRDLDDQMVVIE
                     AATQVAVTVSRVVALAAVARGGRDRAARTREEMAQGQLRDALDKQKATIQRLEHELRQ
                     DFR"
     gene            complement(2454..>4443)
                     /locus_tag="VF516_46165"
     CDS             complement(2454..>4443)
                     /locus_tag="VF516_46165"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HEX8115223.1"
                     /translation="STARARGHTVTQAGPALRSAGDSHIGKVRTTHEDALIVEPRLGL
                     YAVLDGMGGANAGDVASQLARDAIRDFVHQRRTALPPRELLEAAIAAGSAAVFGESQR
                     HRDRHGMGTTVVACLVADAQRVVIGHVGDSRAYLWRDGRLQALTRDHTIVEELVDRGL
                     LSAEEAERHPYKNVLSRNLGARPETRADCLELELRPGDRLLLCSDGLYGYASTEAIQY
                     VLGSGDAPEHVARDLIDLALRGGGGDNVSALVIEAPAAAPSSTQVVRTSGALAWWQHR
                     QRFLQVASDRGLTHNPIARGLPASEAIDVIALSLCEAIYHDLEKSTGVNVWTFAQNLA
                     AGWFERGGDWKAVRGMIDILATSARVVVDDIRGADARLGFLIDVAVSRALIVVELALG
                     GLLAERLRQVDADLIELYSASHDTIEQTTEDSVLDPTGAVNDRGSERFDSLVDPTGGF
                     VPRPAGGAGRVPTPHLLDSLVDRPTVPFLRPDRPITNAGESAEVLTAIGQVITLARGR
                     ISPRARLLEQVLGVLETVVGDSAGNFAAAVLGARELYGVRTVDNAGVMPLFDALDQAR
                     ILVASSVHQLRTPPAIRARALRALSTAHQRLVGATTGLVLEAVHPFTDRLREAQAVTS
                     ELRDAVAKAERRRADLERRFATIVDPSLPWGARGALDG"
BASE COUNT          653 a         1601 c         1542 g          647 t
ORIGIN      
        1 acgaccgcgc tgcgcggcgc ggtcacgcct cgaacacgcg gcggagctgg ccctggccga
       61 cgcgcagcgc gccgccgagc agctcgcgcg gccacgagtg gccggtgagc tcggcgatcc
      121 gccgctgtgc cgcgcggcga tcgatgaacg cgtcggtgaa ccgccgcgga tcggcgaagt
      181 tctcgacgat cgcgttcgcg atccgctgct tgggcgagtc gccctcgccg gtgctgccgt
      241 gctgcgagat cagcatcagc cggcccgccg gggtcagcgg ctcgagcagc gcgttgttga
      301 acgtcacggt cggcgaaccg tgatcggccc agaacgcgtc gaacgtgcgg accatccacg
      361 ccgcgtcgaa cgtgaccccg ggatcggcgg cgatcgcctg ggcgaggtga cggacgagct
      421 tggcgccgag gttggcgccc tggccgccga tcgggtcgac cgacatcgcg gtgtcaccga
      481 tcggcatcac gacccggccc gagggcagcc ggccgaacgg ctcgcggatc gtgggcttga
      541 ccgagcccat cagccagccg ttgcgatcgg cgagctccat gttgcgggcc cactggtggt
      601 cccacgggat cagctcggcc agcacggtct tggccatcgc gacgacctcg gcgccggacc
      661 tgcagccatc gaaccgatcc atcgcgctgt cgggcctggc ctcgaacagc atgaccagcg
      721 tcggcccgtg gtccttgtga tggtacggca tccagatcac ctcgccgtgc tgtgcgagca
      781 ggtggatctt gaccgcctcg aacggggggc cgtcgcacgc gccgtcggcg ccggtcacga
      841 tcaccatcgc cagcttgcgc tgcgcggcgg tgtagacgct gcgccgcgcg ttgcgcggga
      901 acagctcggc gagcgcaccc cgcccgctcg cgaccagcac gaggtcgtgc tgcgcggcga
      961 tcgcatcgag ccggtcgatg tcgatggtct cgatcacgat ccgccccccg gcggcctcga
     1021 ggtcgttcat ccagcggtgg ctctgcagac ggagatcgac cgcctggaag tacgtcgatg
     1081 cgaggacgcc gttcagcgtg accatgcggt tacctggcga cggacagaac gtgaggtgga
     1141 tgccgttgca cttcgctccg gccgcctccc agtgggcgag cccgagctct cgctcgtagc
     1201 cgagcgcgag ctcgaagcgc gccgcggcgc cggtcggccg cgactcgtcg agccactgcg
     1261 ttgcagaccg atcggtgtac agcgtgatct cgtgccccgc cttgcgcagc gcgtgtgccg
     1321 cgagcaaccc cgagagcccc gatcccacga tcgctattcg acgtgccgtc atagtctcag
     1381 agattgcccc atctgcattc gaatcacgag aatataatcc atgcacacac acccacaccc
     1441 ggttgcgggt gaaaacttcc attgaagttt atttctattg cgggttgcgt catattacgt
     1501 cgacggaacg agatcgggag tggtcattgc gcaaccactc tccgcatccg ggcacaacgc
     1561 aacgcactgc ccatcacgag gccggcgcgg caccgtgtcg atcacaatac aggcacaaca
     1621 cagaacagat cactgcgatc gcgctgccgt gtagcagcca ccacgactgc ttctgttgtg
     1681 gaacgtacgc gttcgcggaa cgaccacagg acccgatccc ggttttagca gctgtttcga
     1741 atccgggcca ccggtcacgg tgctctcacg ccggccgcac gggcggtgac ggccgggcat
     1801 gaggcgttgt ctagctgaac cggtgaaaag tttcacccgg gatgcttcga ttacggaaca
     1861 acgtcaggcg ggttacagcg cgggccgatg tatgccaccc gatgtatgcc agatgctgcc
     1921 aggttccgac ctgcgcgggc taccgaaagt cttgcctgag ctcgtgctcg aggcgctgga
     1981 tcgtggcctt ttgcttgtcc agcgcgtccc ggagctggcc ctgcgccatc tcctcacggg
     2041 ttcgcgcggc gcgatcgcga ccgccgcggg cgaccgcggc gagcgcgaca acccgcgaca
     2101 cggtgaccgc gacctgggtc gcagcctcga tcaccaccat ctgatcgtcg agatcgcgga
     2161 atgcgcgctc gaccgacacc atcagccgat cgaacaccag ctcggcgtcg gcgagcaccg
     2221 cctcctcgct gtcgccccac gccagccggc gcgccaccgc atcgtgcagc gtgagatcga
     2281 ggccgggaac cgactcgccg ccgatcgcca gcgcgtggag cagcgcgtcg aacacctcgg
     2341 cacccggcgg atcgcggtcg ccgtggtgca cggtatcgag gacttcgtcg cgcagcacgc
     2401 gcacggcttc ggtcagatcg acgtcggaca tgctggctcc tgtgcgctcg cggtcacccg
     2461 tccagggcgc cccgggcgcc ccacggcagg ctcgggtcga cgatcgtcgc gaaccggcgc
     2521 tcgagatcgg cgcgccggcg ctcggccttg gccaccgcat cgcgcagctc gctggtcacc
     2581 gcctgggcct cccgcaggcg gtcggtgaac gggtggaccg cctcgagcac caggccggtg
     2641 gtcgcaccga ccaggcgctg gtgcgcggtg ctgagcgccc gcagcgcgcg cgcccggatc
     2701 gccggcggcg tgcgcagctg gtggacgctc gacgcgacca ggatccgcgc ctggtcgagc
     2761 gcgtcgaaca gcggcatcac cccggcattg tccaccgtcc tgacgccgta cagctcgcgc
     2821 gcgccgagca ccgcggcggc gaagttgccc gcgctgtcgc cgaccacggt ctcgagcacg
     2881 ccgagcacct gctcgagcag ccgggcgcgc ggcgagatcc ggccgcgcgc gagcgtgatg
     2941 acctggccga tcgcggtcag gacctcggcc gactcgccgg cgttggtgat cggccggtcc
     3001 gggcggagga acgggaccgt cgggcgatcg acgagcgagt cgaggagatg gggggtgggg
     3061 accctgccgg ccccgccggc ggggcggggg acaaagcccc ccgtgggatc gacgagcgaa
     3121 tcgaaccgct cgcttccccg atcgttcacg gccccggtgg ggtcgagcac cgagtcctcg
     3181 gtggtctgct cgatcgtgtc gtggctcgcg ctgtacagct cgatcaggtc ggcgtcgacc
     3241 tggcgcagcc gctcggcgag caggccgccg agcgcgagct cgacgacgat cagcgcgcgc
     3301 gacaccgcga cgtcgatcag gaacccgagc cgggcgtccg caccgcggat gtcgtcgacc
     3361 acgacccgcg cgctggtcgc gaggatgtcg atcatgccgc gcaccgcctt ccagtcgccg
     3421 ccgcgctcga accagccggc cgcgaggttc tgggcgaacg tccacacgtt gactccggtc
     3481 gacttctcca ggtcgtggta gatcgcctcg cacagcgaca gcgcgatcac gtcgatcgcc
     3541 tcgctggccg gcagcccgcg cgcgatcggg ttatgggtca ggccgcggtc gctcgcgacc
     3601 tggaggaagc gctggcggtg ctgccaccac gccagcgcgc cgctggtccg gaccacctgg
     3661 gtcgacgacg gcgcggccgc cggcgcctcg atcaccagcg ccgagacgtt gtcgccgccg
     3721 ccgccgcgca gcgcgaggtc gatcaggtcg cgcgccacgt gctcgggggc gtcgcccgat
     3781 cccagcacgt actggatcgc ctcggtcgac gcgtagccgt acaggccgtc ggagcacagc
     3841 agcagccggt cgcccggccg gagctcgagc tccagacaat cggcccgggt ctcgggcctg
     3901 gcgccgaggt tgcgcgacag cacgttcttg taggggtgcc gctcggcctc ctcggcggac
     3961 agcaggcccc ggtcgaccag ctcctcgacg atcgtgtgat cacgggtcag cgcctgcagc
     4021 cggccgtcgc gccacaggta cgcgcggctg tcgccgacgt ggccgatcac gacgcgctgg
     4081 gcgtcggcca ccaggcacgc gaccaccgtc gtgcccatgc cgtggcgatc gcgatggcgc
     4141 tgcgactcgc cgaacaccgc cgccgagccc gcggcgatcg cggcctcgag cagctcgcgc
     4201 ggtggcagcg cggtgcggcg ctggtgcacg aagtcgcgga tcgcatcgcg cgccagctgc
     4261 gaggcgacgt cgccggcgtt ggccccgccc atgccgtcga gcacggcgta cagcccgagc
     4321 cgcggctcca cgatcagcgc gtcctcgtga gtcgtccgga ccttgccgat gtggctgtcg
     4381 ccggcgctcc gcagcgccgg acccgcctgg gtcacggtgt gccctcgcgc gcgcgccgtg
     4441 ctc
//
LOCUS       DASVOR010002516         5412 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_348830,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002516 DASVOR010000000
VERSION     DASVOR010002516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 5412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5412
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348830"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VF516_46170"
     CDS             <1..535
                     /locus_tag="VF516_46170"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HEX8115224.1"
                     /translation="LADPAQRRQHSAVTPTGWSSLAPGLGQGNYGVSYEARAAVAAIG
                     LGANLRIDAIYLNASVDAGGQPLDGSKRYRLRFAAGSAPPVHAFWSITLYDEQGYLVA
                     NPIARYAVKSGDPLVTEPDGALVIDLAPDDPGPERRASWLPTPAHQRFELSLRAYWPD
                     DALLHDRWSPPAVVPAP"
     gene            638..1657
                     /locus_tag="VF516_46175"
     CDS             638..1657
                     /locus_tag="VF516_46175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115225.1"
                     /translation="MFDWLRKKRRDAPPASGHVHIEGQQTSDLDPVALVADRYSPVPG
                     AQFKALDPAQTADLAARAGEGKPIPFGRWVWMELPWELKERFLGYVYLDPEAGPSAKG
                     GPESASQIAELPTYTVRLPMRLPMQLLTPEEITKRALPVIPAWLHHYGPQPDPEAAWR
                     KDPDLKGRFHDSYPDDLQVLVHDGEPRRTSRQPELCWVRIVEAKEGPPRRFIFMPQAT
                     SLSESEFARKYRTNKIVYVGKLLNAPHALQSVRQGDTVRFFTGGGLEQPLQVTLEYLE
                     ERENWRIVPCNKCGMGECLDPPSVMAKLRFPDQPPDAVTTMFTSFCPRCGGMQMLSRH
                     DQAEQ"
     gene            complement(1793..2767)
                     /locus_tag="VF516_46180"
     CDS             complement(1793..2767)
                     /locus_tag="VF516_46180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115226.1"
                     /translation="MIDWQQLLQGNLPAVHGVRVGDPKKAVPVDANTTTDAVEAGEWR
                     RAGGPSFLDPCCFVLKNGRVARILVRGTLLDGLPIREQEDITRLFGEPLGIERIFRWR
                     FIHYYPERQLSIAWNPVEKRVEQVAFGPVTWEPRMLSARDVLDEWLASDLSWKPDATE
                     PSDRSSSAWVRWMRVHALLRGFQLGSPIEFARGEFLLESDPDNCPHACNAIRKHESDL
                     FPWDREREQMELGHLFARLLGYRRRAHQLLQFNSGTLEAGFPGTIASIKITEAMNRRI
                     ATEAAAIDRLLVELIGPGDLRVAERDLEGWIYDDELKYLVEVETWGPE"
     gene            complement(2851..3615)
                     /locus_tag="VF516_46185"
     CDS             complement(2851..3615)
                     /locus_tag="VF516_46185"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEX8115227.1"
                     /translation="MRSLAVVSLLAAGAAFAEPVRAPFHVRYDADHLDLAGHVLQFQM
                     SRPAGSAELVAIGEDGQQLGTGSASYDHPPPDTWLSVSWTQPAEARVLKLQLRAVSAD
                     GLVTRVELIPWSVTIDHEDVNFATNSFAIESSEEAKLDASLTRIAEVVKRSEKFVKMQ
                     LYVAGHTDTVGPAGKNLALSQGRARAIAAYFRRKGLALPIVFAGFGEQVLKVATPDET
                     DERRNRRADYVIAPRGAPPPFKGPYLKARVSWKQLP"
     gene            complement(3628..4647)
                     /locus_tag="VF516_46190"
     CDS             complement(3628..4647)
                     /locus_tag="VF516_46190"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF006589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEX8115228.1"
                     /translation="MFPTTIAGSLPKPAWLAEPDRLWAPWRQSGAELAAAKLDATLLA
                     IKHQEDAGVDVVSDGEQSRQHFVHGFLEQLAGIDFEHKVEIGIRADRYKAMCPVVTGA
                     LHARGRVHEAEARHARAHTARKLKFTLPGPMTIVDTLADQHYGDRVKLAMAFADALNA
                     EARALERDGVDLIQFDEPAFNVYMDDVVGWGIEALHRAIDGLTCTTAVHICYGYGIKA
                     NIDWKATLGGEWRQYEKIFPALAASRIGQVSVECRNARVPMSLLGLLDGKDVMVGVID
                     VATDEVETPEQVADTIEAATRHVPKHRIIASTNCGMAPMRRDIAYAKLAALGRGAALA
                     RARLG"
     gene            4783..>5412
                     /locus_tag="VF516_46195"
     CDS             4783..>5412
                     /locus_tag="VF516_46195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEX8115229.1"
                     /translation="MVLALLAACGGGNHPGWLSDARVVVDGVHAVASDCRTQICVHNE
                     NTDLTVFDGATYLVHRTATSQILGPNSSLRVLRSTDHGNHWRLLAVIPAPEGRDLRDP
                     SFYQIDGKLAIKALTRRQVISLRDSDVDTIAVAGVSSDAGRTWSPLAPIGPERWSFWR
                     IRDDARGVHYTAAYEDGDRSVQLFSSRDGTQWADVSRIYGRAEDTPLETE"
BASE COUNT          869 a         1840 c         1834 g          869 t
ORIGIN      
        1 cctggccgac cccgcgcagc gccggcagca cagcgcggtg acgccgaccg gctggtccag
       61 cctggcgccc gggctcggcc agggcaacta cggcgtgagc tatgaggccc gcgccgcggt
      121 cgccgcgatc gggctcggcg ccaacctgcg gatcgacgcg atctacctga acgcgagcgt
      181 cgacgccggc ggccagccgc tcgacggcag caagcgctac cgcctgcggt tcgccgccgg
      241 cagcgcgccg ccggtgcacg cgttctggtc gatcacgctg tacgacgagc agggctacct
      301 ggtcgcgaac ccgatcgcgc gctacgcggt caagtccggc gatccgctgg tcaccgagcc
      361 cgacggcgcg ctcgtcatcg acctcgcgcc cgacgatccg ggcccggagc gccgcgccag
      421 ctggctgccg acgccggcgc accagcggtt cgagctgtcg ctgcgcgcgt actggcccga
      481 cgacgcgctg ctgcacgacc gctggtcgcc cccggcagtg gtcccagcgc cgtagccggt
      541 ccccaggtgc agtcggatcg cacgacagct acccagtctt gaagttactg ggccggccgg
      601 tcaccatacc tgggacgact gagagaggtg gtgaaacgtg ttcgattggc tgcgcaagaa
      661 gcggcgtgat gcgcccccgg cgagcgggca cgtgcacatc gagggacagc agaccagcga
      721 tctcgaccca gtcgcgctgg tcgcggaccg gtactcgccc gttccgggag cgcagttcaa
      781 agctctcgac ccagcccaaa ccgccgactt ggccgcgcgc gcaggagagg ggaagcccat
      841 cccatttgga cgttgggtct ggatggaact gccctgggaa ctgaaggaac gcttcctcgg
      901 gtacgtgtat ctcgatcccg aggccggacc ttcggccaag ggtggaccag agagcgcgtc
      961 gcaaatcgcg gagcttccaa cgtacacggt gcggctgccg atgcggctgc ccatgcaact
     1021 gctcacgcca gaggagatca ccaaacgagc cttgccggta atcccggcgt ggctgcacca
     1081 ctacggaccc cagcccgatc ctgaagctgc ttggcggaag gacccagact tgaaggggcg
     1141 cttccacgat agctaccccg atgatctgca agttctcgta cacgacggag agccacgccg
     1201 aaccagtcgg cagccggagc tctgctgggt ccgcatcgtc gaagccaagg aggggccgcc
     1261 gcgccgtttc atcttcatgc ctcaagcgac gtcgctctcc gagagcgagt ttgccaggaa
     1321 gtatcggaca aacaagatcg tctatgtcgg caagctgctg aacgcccccc atgccctcca
     1381 gagcgttcgc caaggcgaca cggttcgctt tttcacgggc ggtgggctcg agcagccctt
     1441 gcaggtgacc ttggagtacc tcgaagagcg tgagaactgg cggattgttc cttgcaacaa
     1501 gtgcggcatg ggtgaatgtc tcgatccgcc aagcgtcatg gcgaagctcc ggttccccga
     1561 ccaaccaccg gatgccgtga cgaccatgtt cacctccttc tgccctcgat gcggcggcat
     1621 gcagatgctc tcgcgccatg accaagcaga gcagtgaggc gtccacagcc caggcgctgg
     1681 tttatccgtt cctccgaaca agacccccgt atgggccttc ccgccaggag cgctggtcgc
     1741 cccggcggtg gtcccggcgc catggtcgcc ggccagagga tgacgtttca gctcactccg
     1801 ggccccaggt ctcgacctct accaagtatt tcagctcgtc gtcatagatc caaccttcga
     1861 ggtcgcgctc cgcgacgcga aggtcgccgg gtccgatcag ctcgacgagg agcctatcga
     1921 tggccgcggc ctcggttgcg attcgacgat tcatggcttc cgtgatcttg attgacgcga
     1981 tggttccggg aaacccagcc tccaaggttc cagagttgaa ctgcagcagt tgatgtgctc
     2041 ttctacgata gcccagcaac cgcgcgaaca ggtgacctaa ctccatctgc tccctttcgc
     2101 ggtcccaggg gaagaggtca ctctcgtgct tgcggatcgc attgcaggca tgagggcagt
     2161 tgtccggatc gctctccaac aagaactcgc cccgggcaaa ttcgatggga gaccccagtt
     2221 gaaaaccacg caggagcgca tgcacgcgca tccagcgaac ccaggcagag ctggatcgat
     2281 cgctcggttc cgttgcgtcc ggtttccatg acaggtcgga ggccaaccat tcgtcgagca
     2341 cgtcgcgtgc ggacagcatc cgcggctccc aggtcaccgg gccgaaggcg acctgctcga
     2401 cgcgcttctc cacggggttc caggcaatgg agagctggcg ctcaggatag taatggatga
     2461 acctccagcg aaagatacgt tcaataccga gcggctcccc gaacaggcgc gtgatatctt
     2521 cctgttcccg gatgggaagg ccatccagga gtgtgcctcg caccaggatc ctcgcaacac
     2581 ggccgttctt gaggacgaag caacacgggt cgaggaagga agggccgccg gcgcgccgcc
     2641 actctcccgc ttccactgca tcggtcgtcg tgtttgcatc gactggaacg gctttcttcg
     2701 gatcacctac acggacgccg tgtacggctg gtaggttccc ttgcaagagc tgttgccagt
     2761 cgatcatggg cgaggccgag cgtactcgcg caggcttcgc gcgccaacgc ggatcgtcgc
     2821 ggcggcggca tccctggacc gccgtcgccc ttacggtagc tgcttccagg acacccgcgc
     2881 cttcaggtac gggcccttga acggcggcgg cgcgccgcgc ggcgcgatca cgtagtcggc
     2941 gcggcggttg cggcgctcgt cggtctcgtc gggcgtggcg accttcagca cctgctcgcc
     3001 gaacccggcg aacacgatcg gcagcgcgag ccccttgcgg cggaagtacg cggcgatcgc
     3061 gcgcgcgcgg ccctgcgaca gcgcgaggtt cttgccggcc ggtccgacgg tgtcggtgtg
     3121 gccggcgacg tagagctgca tcttgacgaa cttctccgag cgcttcacga cctcggcgat
     3181 ccgggtcagg ctcgcgtcga gcttggcctc ctcggaggac tcgatcgcaa agctgttggt
     3241 cgcgaagttg acgtcctcgt ggtcgatggt gaccgaccac gggatcagct ccacccgcgt
     3301 gaccaggccg tcggcggaca ccgcgcggag ctggagcttg agcacgcggg cctcggccgg
     3361 ctgggtccac gacaccgaca gccaggtgtc gggcggcgga tggtcgtagc tggccgagcc
     3421 ggtgccgagc tgctggccgt cctcaccgat cgcgaccagc tcggcgctcc ccgccgggcg
     3481 cgacatctgg aactgcagca cgtggccggc gagatcgagg tgatcggcgt cgtagcggac
     3541 gtggaacggc gcgcgcaccg gctcggcgaa cgccgcgccc gcggcgagca gagagacgac
     3601 ggcgaggctg cgcatgtcgc gatgatgtca tcccagccgc gcgcgggcca gcgcggcgcc
     3661 ccggccgagc gcggcgagct tggcgtacgc gatgtcgcgc cgcatcggcg ccatgccgca
     3721 gttggtgctc gcgatgatcc ggtgcttcgg cacgtggcgc gtcgcggcct cgatcgtgtc
     3781 ggcgacctgc tcgggggtct cgacctcgtc ggtcgcgacg tcgatcacgc cgaccatcac
     3841 gtccttgcca tcgagcaggc cgagcaggct catcggcacc ctggcgttgc ggcactcgac
     3901 cgacacctgg ccgatccggc tggcggcgag cgccgggaag atcttctcgt actggcgcca
     3961 ctcgccgccg agcgtggcct tccagtcgat gttggccttg atgccgtagc cgtagcagat
     4021 gtgcaccgcg gtggtgcagg tcaggccgtc gatcgcgcgg tgcagcgcct cgatgcccca
     4081 gccgaccacg tcgtccatgt agacgttgaa cgccggctcg tcgaactgga tcaggtcgac
     4141 cccgtcgcgc tcgagcgcgc gggcctcggc gttgagcgcg tcggcgaacg ccatcgccag
     4201 cttcacgcga tcgccgtagt gctggtcggc gagggtgtcg acgatcgtca tcgggccggg
     4261 caaggtgaac ttgagcttgc gcgcggtgtg cgcgcgggcg tggcgcgcct cggcctcgtg
     4321 cacgcggccc cgggcgtgca gcgcgccggt gacgaccggg cacatcgcct tgtagcggtc
     4381 ggcgcggatg ccgatctcga ccttgtgctc gaagtcgatg ccggcgagct gctcgaggaa
     4441 gccgtgcacg aagtgctggc gcgactgctc gccgtcgctc accacgtcga cgccggcgtc
     4501 ctcctgatgc ttgatcgcga gcagcgtcgc gtcgagcttg gcggcggcga gctcggcgcc
     4561 ggactgtcgc cacggtgccc acaggcggtc gggttcggcg agccaggcgg gcttgggcag
     4621 gctgccggcg atggtggtgg ggaacatgag ggcgccacca tacctcgaac ccgaggcgcg
     4681 gtcggcctcc ccatcgcctc acggtgacat tttcgtcgca gccaggaatc gaacagggtc
     4741 gctgcggtga cgatgtcata acagacggtc atgagaggtt gtgtggtcct ggcactgctc
     4801 gccgcgtgcg gcggcggcaa ccatcccggc tggttgagcg atgcgcgggt ggtggtcgac
     4861 ggggtccacg cggtggcgag cgattgccgg acccagatct gcgtccacaa cgagaacacc
     4921 gacctgacgg tgttcgacgg tgccacctat ctcgtccacc ggaccgcgac cagccagatc
     4981 ctcgggccga acagctcgct gcgggtgctg cggtccaccg atcacggcaa ccactggcgg
     5041 ctgctcgcgg tgatcccggc gccggagggc cgcgatctgc gcgatccgag cttctaccag
     5101 atcgacggca agctcgcgat caaggcgctg acccgccggc aggtgatctc gctgcgcgac
     5161 agcgacgtcg acacgatcgc ggtcgccggc gtgtcgtccg acgccggccg gacgtggagc
     5221 ccgctcgcgc cgatcggccc cgagcgctgg agcttctggc ggatccgcga cgacgcccgc
     5281 ggcgtccact acaccgcggc ctacgaggac ggcgaccgca gcgtccagct gttctcgtcg
     5341 cgcgacggca cgcagtgggc cgacgtgtcg cggatctacg gccgcgccga ggacaccccg
     5401 ctcgagaccg ag
//
LOCUS       DASVOR010002517         3485 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_116832,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002517 DASVOR010000000
VERSION     DASVOR010002517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116832"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1007)
                     /locus_tag="VF516_46200"
     CDS             complement(60..1007)
                     /locus_tag="VF516_46200"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEX8115230.1"
                     /translation="MSLDDSDDSLQMDSIRIDRRKDRHDHAWELFQLLEQVPVGVFVA
                     TGEGRPYYANAHARALLGMGAVPDHVDRFSEVYQAFEIGTDQLYPIERLPLHRALVGE
                     RCEISDIELRRHGDLIPLHVSGAPIKRDGGVAFAIMALQDVRELRRIATQDALTGLPN
                     RRAVTEAFARERLRAERSKKPIAVALIDFDRFKSINDEFGHAGGDGVLRRGAAAIVEA
                     LRPTDIVGRWGGEELLVVLPNTRLEVARRAIERALTAVRALDMPLKGTRVHTTFSAGV
                     VEMTGNEALDEIVARADVALYEAKRAGRDRVVAATAEPG"
     gene            complement(1124..1927)
                     /locus_tag="VF516_46205"
     CDS             complement(1124..1927)
                     /locus_tag="VF516_46205"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HEX8115231.1"
                     /translation="MISKLSVDRDDHVAVVSLLAPTMPPALFAELGEVFAELSRDAAL
                     RAVILRGGGKVFSYGLDLPAAFAAWGPAFSGQGLAASRAELWNLVRDIQATITAVASC
                     PAPVIAAVHGWCVGGGVDLITACDLRICSADARFSVRETKVAMVADLGSLQRLPLVIG
                     DAATRELALTGKDIDASRALALGLVSQIATDRAALDAAARALAAEIAGNPPLVVRGVK
                     QILDQATRRDVAEGLDRVALWNAAFLPSEDLAEAANAFAARRTPVWKGR"
     gene            complement(1992..3134)
                     /locus_tag="VF516_46210"
     CDS             complement(1992..3134)
                     /locus_tag="VF516_46210"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HEX8115232.1"
                     /translation="MATHQRFLTTHIGSLPRSEELIQIMFAREDGIPLDRAALEDRIA
                     LAVDGVVAHQLEAGIDILNDGEMSKPSYATYVKDRLNGFGGTGNSFVFADLEAYPHTK
                     ARVFADPGRKHRKTPACNGPISMRDVEAPRRDAQRLQQALHGKQPAGLFLSSASPGVT
                     SFFFRNDFYPSHQDYVFAIAEALRHEYEAIVDAGATLQVDCPDLAMGRHTQFAHLDVK
                     GFREKMELHIEALNRALVNIPAERVRMHLCWGNYPGPHHCDVPLADIVDLVWKAKPHA
                     ILFEAANPRHAHEWVVFEHVKVPDGKLLIPGVIECQSNYIEHPELVAQRISRYAHLVG
                     RDRLMAAVDCGFSIHVGSGGVDPDVVWAKLASLAQGAAIASKTFWP"
     gene            3199..>3485
                     /locus_tag="VF516_46215"
     CDS             3199..>3485
                     /locus_tag="VF516_46215"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115233.1"
                     /translation="MADPTLPLLPTTVVGSYPQPDWLIDREALLSHTPPRVRMRQVWR
                     VDERFLEAAQDDATRLAIADMERAGLDILTDGEIRRESYFNRFATALSGID"
BASE COUNT          497 a         1247 c         1234 g          507 t
ORIGIN      
        1 cctggcgctg gcgcgcgcat ggctcggcga gcctgcgccg ggcggatagc ggctgccgct
       61 cagcccggct cggcggtcgc ggcgaccacg cgatcgcggc cggcgcgctt ggcctcgtag
      121 agcgcgacgt cggcgcgcgc cacgatctcg tcgagcgcct cgttgccggt catctcgacg
      181 acgcccgccg agaacgtggt gtgcacgcgg gtgcccttca ggggcatgtc gagcgcgcgg
      241 accgcggtga gcgctcgctc gatcgcccgg cgcgccacct ccaggcgggt gttcggcagg
      301 accaccagga gctcctcgcc gccccaccgg ccgacgatgt cggtcggtcg cagcgcctcg
      361 acgatcgccg ccgcgccgcg ccgcagcacc ccgtcgccgc cggcgtggcc gaactcgtca
      421 ttgatcgact tgaagcgatc gaagtcgatc agcgccaccg cgatcggctt cttgctgcgc
      481 tcggcgcgca gccgctcgcg ggcgaacgcc tcggtgaccg ccctgcggtt gggcagcccg
      541 gtgagcgcgt cctgggtcgc gatccgccgc agctcgcgga cgtcctgcag cgccatgatc
      601 gcgaaggcga ccccgccgtc gcgcttgatc ggcgcgccgc tgacgtgcag cgggatcagg
      661 tcgccatggc ggcggagctc gatgtcgctg atctcgcagc gctcaccgac cagcgcgcgg
      721 tgcagcggca ggcgctcgat cggatacagc tggtcggtcc cgatctcgaa cgcctggtag
      781 acctcgctga accggtcgac atggtccggc accgcgccca tgccgagcag cgcgcgcgcg
      841 tgcgcgttcg cgtagtacgg ccggccctcg ccggtcgcga cgaacacccc gaccgggacc
      901 tgctcgagca gctggaacag ctcccaggcg tgatcgtgcc ggtcctttcg ccgatcgatc
      961 cggatcgaat ccatttgtaa cgagtcgtcg gaatcgtcga gggacatgaa gacggtagtc
     1021 taacgcgact ccacatctcc aggcacccgg cgcgcggcgg cgttcgcgcg tctgcgatac
     1081 ccacgtcgtg ggcaggtctc gcaggtcgtc cgccgcggtg ggctcagcgc cccttccaca
     1141 ccggtgtccg gcgggccgcg aacgcgttcg ccgcctccgc gaggtcctcc gacggcagga
     1201 acgctgcgtt ccacagcgcg acccgatcca gcccctcggc gacatcgcgc cgcgtcgcct
     1261 ggtcgaggat ctgcttgacg ccgcgcacga ccagcggcgg attgccggcg atctccgcgg
     1321 cgagcgcacg cgcggcggca tcgagcgcgg cgcggtcggt ggcgatctgg ctgaccaggc
     1381 cgagcgcgag cgcacgcgac gcatcgatgt ccttgccggt cagtgccagc tcacgcgtcg
     1441 cggcatcgcc gatcaccagc ggcagccgct gcaggctgcc gaggtccgcg accatcgcga
     1501 ccttggtctc gcgcaccgag aaccgcgcgt cggcgctgca gatccgcaga tcgcacgccg
     1561 tgatcagatc gacaccgccg cccacgcacc agccgtggac cgccgcgatc accggcgccg
     1621 ggcagctcgc gaccgccgtg atcgtcgcct ggatgtcgcg gaccaggttc cacagctcgg
     1681 cgcgcgacgc cgcgagcccc tgacccgaga acgccggccc ccaggccgcg aacgccgccg
     1741 gcagatcgag cccgtagctg aacaccttgc cgccgccgcg gaggatcacc gcgcgcagcg
     1801 cggcatcgcg cgacagctcg gcgaacacct cgccgagctc ggcgaacagc gcgggcggca
     1861 tggtcggcgc gagcagggat acgacggcga cgtgatcgtc tcgatcgacg ctgagcttgc
     1921 tgatcattcc ggacacgttg ccaccatcgc gccccccgat cgaggggggc aagcgcagcg
     1981 caccctcacg gtcacggcca gaacgtcttg ctggcgatcg ccgcgccctg cgccagcgac
     2041 gcgagcttgg cccagaccac atcgggatcg acgccgcccg agccgacgtg gatgctgaag
     2101 ccgcagtcga ccgcggccat caggcggtcg cggccgacca ggtgggcgta gcggctgatc
     2161 cgctgggcga cgagctcggg gtgctcgatg tagttggact ggcactcgat caccccgggg
     2221 atcagcagct tgccgtcggg caccttgacg tgctcgaaca ccacccactc gtgggcgtgg
     2281 cgcgggttgg cggcctcgaa caggatcgcg tgcggcttgg ccttccacac gaggtcgacg
     2341 atgtcggcga gcggcacgtc gcagtgatgc gggcccgggt agttgcccca gcacaggtgc
     2401 atgcgcaccc gctcggcggg gatgttgacc agcgcgcggt tgagcgcctc gatgtgaagc
     2461 tccatcttct cccggaagcc cttgacgtcg aggtgggcga actgggtgtg gcggcccatc
     2521 gcgagatccg ggcagtcgac ctggagcgtc gcgccggcgt cgacgatcgc ctcgtactcg
     2581 tggcgcagcg cctcggcgat cgcgaacacg tagtcctggt ggctcgggta gaagtcgttg
     2641 cggaagaaga acgacgtcac gcccggggac gccgagctca ggaacaggcc ggccggctgc
     2701 ttgccgtgca gcgcctgctg caggcgctgg gcgtcgcggc gcggcgcctc gacgtcgcgc
     2761 atgctgatcg ggccgttgca cgccggggtc ttgcgatgct tgcggcccgg atcggcgaac
     2821 acgcgcgcct tggtgtgggg gtacgcctcg aggtcggcga acacgaagct gttgccggtc
     2881 ccgccgaagc cgttcagccg gtccttgacg taggtcgcgt agctcggctt ggacatctcg
     2941 ccgtcgttga ggatgtcgat gccggcctcc agctggtggg cgacgacgcc gtcgacggcc
     3001 agggcgatcc ggtcctccag cgcggcgcgg tcgagcggga tgccgtcctc gcgggcgaac
     3061 atgatctgga tcagctcctc gctgcgcggg aggctgccga tatgggtggt cagaaaacgc
     3121 tggtgggtgg ccatcgcgat cctcgggtcg ttggtaacac atcgccggcg ggaagcggct
     3181 aagctcgcgc cgcaggcgat ggctgacccg accctcccgc tgttgccgac caccgtcgtc
     3241 ggctcctatc cgcaacccga ctggctgatc gatcgcgagg ccctgctgtc tcacaccccg
     3301 ccgcgggtgc ggatgcgcca ggtatggcgg gtcgacgagc gcttcctcga ggcggcgcag
     3361 gacgacgcga cccggctggc gatcgccgac atggagcgcg ccgggctcga catcctgacc
     3421 gacggcgaga tccggcgcga gagctacttc aaccggttcg ccaccgcgct gtccggcatc
     3481 gacat
//
LOCUS       DASVOR010002518         6449 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_406904,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002518 DASVOR010000000
VERSION     DASVOR010002518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 6449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6449
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406904"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..2123
                     /locus_tag="VF516_46220"
     CDS             138..2123
                     /locus_tag="VF516_46220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEX8115234.1"
                     /translation="MPKVVVAPSQTIAEIVREHEETRPVFLRHGLALDEIASRSLAEV
                     AAEHGLDPAALERELVHAIEESEPFAHGGFEWEAAIGVMRVSSSLAAMFEMAELSGPA
                     SVFLARVHPDDRDRVQELFREARERPHPFAFEYRVVHTDGSTRIYASQVEPLDGASRF
                     IGMCWDITEQHPCEADPGPLLRNTLEATADGILVIDRAGNITTYNQRFLSLWGIPNEL
                     VATGRDEAMLRYVLDQFSDPEQFLGRVHALYTNPERESFDVLRFKDGRVFERYSRPQR
                     VAFAIVGRVWSFRDVTERERLLTAKSLLADAGRLLTSLDPRSALDAVVRLAVPGLGDA
                     GAIELFGEPKIAASYSIDPAQSFSPAWPAGVLAGSSAMSDRDGVSELAVPLVIQDEAI
                     GAVAFAAPSSRRHTLADLDLAERLAHGIALSIENAWLYDHARQALRAREEFLAIAAHE
                     LRGPLSTMNLAVQTLKRRDVPQPTRDRMLSLIEREDRRLTRFADDITEIARIRAGALH
                     FDDDEVDLVQIAREVVARLAPDAERSGSTVLIDAPHPVVGRWDRMRLDQVVTNLVINA
                     FKFGLGRPIELSVRARDGWATLKVSDQGLGVRASQREAIFRPFERAVPVRHYGGLGLG
                     LFIVRTIVTRYGGTVHVEPRDSGGSCFVVRLPQAGVA"
     gene            2120..2533
                     /locus_tag="VF516_46225"
     CDS             2120..2533
                     /locus_tag="VF516_46225"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEX8115235.1"
                     /translation="MIDGRRAVLVVDDDPDILEIVTVVLGLYDVPAVTACDGIEALHA
                     LRGSAELGLVLLDLMMPRMNGVDVLAEMKRDPALAAMPVIVISGNYHTEQDVLAMGAD
                     EYLLKPLDVDVLMRVVSRFVPVTGLRPEPDHGHGQ"
     gene            2775..>6449
                     /locus_tag="VF516_46230"
     CDS             2775..>6449
                     /locus_tag="VF516_46230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8115236.1"
                     /translation="MPSVRLTREGSRYDVRRDRRQGRSVIIKRQKDPMDERASALLRH
                     EYHTLQSIETPGVVKPLALDELNGRPALILEDAGDENLAAHLTRGALDLDAFLALAVQ
                     MAAIVARVHQRNILHRDLCPDNFVLDDGRVTLVDFESATTATGAVAVEPVGALLYMAP
                     EATGRLRRGPDERSDLYSLGAIFYEMLTGRPPFPSVDPVELVHAHLARNPVAPAILDP
                     CVPRPVSDIALRLLAKDPDARYQTSAGLLADLEEARAQWLSGRTIPPFELGRDQLMRE
                     FPPPARLYGREPAEAVLAGAVERVSSGATELFALTGRAGVGKSALVAQLAATAQRDGR
                     WVAGKCDLLAGNLPYAPLLELFGELVQELLAASEAERADLRDRILRALAPNARVLTDF
                     LPGLIPLLGEPSPVPALGPAETQTRFHMVMQSFVRAVAAARPLIGFLDDAQWADAATL
                     TLLRVLAETPQLRHCLIIVAYRSEDVGEHHATHLAIEAIRAAGVAVHAMELAPLDVAD
                     ITALLADALRIAPEAAAPLGEAVHGKTAGNPLFVRRFLRYLHDAGLFVFDRQTGVWQW
                     DLARIAEAPVTENVVDLLMSAIARLPGECRRALAVAACIGARFELDVVAAVSGASLEA
                     TGCALAPALREQLIVPVWPASPGGPATYGFAHDRIQQAVHSLESEDARQRTHLRIGRR
                     LAAAATGSAVPPFAIADQLNLAIGAMTDPAERLGLAELDLRAGTAAKARSAHGPALAY
                     LTHGLELLPERAWATHHELWYRLHREAVECAGLDGKHARADALVEAALPHTGSLEEKA
                     ELYALQIMGATLGGSLPHALRVLGDGLLAFGVTVPRDDLPAAAIAELAAARDALTESA
                     ADWLLQAPPMTGDELSVVRILASALPAGSYVDPDLLAWLSARLVSSTLRHGPAPQTSL
                     GLGMFAFALLNVHDYRMAERAGQLAIRVAERLRDPVATSRARFMYAGLMSFWLQPIRN
                     SIPLLQRAGGELLALGDHQFAPWNSQDLASLLLAQGSPLDEVLAEVDHGLALAKRFDH
                     APVRRFFALFRTGLRDLVGTARGQDADLLATASPLNAGLYHILQLQTAYILGDLAAAD
                     TAARAAAEQLPFLAGVFFWIVDYALYDALTAAARLDSAGADERAALRARIRDHLRELE
                     AWAELCPDNVLHKVRLLAGEIAAIDHQPLDAARLYEEAIDRAGREGFVHDAALAHERC
                     GRMYLRVGLKREAEAHLADAI"
BASE COUNT          864 a         2313 c         2295 g          977 t
ORIGIN      
        1 tcgcaccggc gcccggtcgg gcacgcgctc gcggcccgtc gccggcgccg gtggagcgca
       61 gcgggagccc tccggcgctc gcgtcccgct ctcacgcgcg tcacatcctg gcacgcgcga
      121 tgctcggatc tgcgggcatg ccgaaggtgg tcgtcgcccc atctcaaacg atcgccgaga
      181 tcgttcgcga gcacgaggag acgcggccgg tgttcctgcg ccacggcctc gcgctcgacg
      241 agatcgcgtc ccggtcgctt gcggaggtgg ccgcggagca cggtctggat ccggcggccc
      301 tcgagcgcga gctcgtgcat gcgatcgagg agtccgagcc gttcgcccac ggcggcttcg
      361 aatgggaagc ggcgatcggg gtcatgcgag tctcttcgag cctggccgcc atgttcgaga
      421 tggccgagct cagcggaccg gcgagcgtgt tcctcgcgcg ggttcacccc gacgaccgcg
      481 accgggtgca ggagctgttc cgcgaggcgc gcgagcggcc tcatccgttc gcgttcgagt
      541 accgggtcgt gcacaccgat ggttcgaccc ggatctacgc ctcgcaggtc gaaccgctcg
      601 acggcgcatc gcggttcatc ggcatgtgct gggacatcac cgagcagcat ccctgcgagg
      661 ccgatccggg accgctgctg cgcaacaccc tggaggcgac cgccgatggc atcctggtga
      721 tcgatcgcgc cggcaacatc accacctaca accagcgctt cctgtcgctg tggggcatcc
      781 cgaacgagct ggtcgcgacc ggccgcgacg aggcgatgct ccgctacgtg ctcgaccagt
      841 tcagcgatcc cgagcaattc ctcggtcgtg tgcacgcgct gtacaccaac cccgagcgcg
      901 agagcttcga tgtgttgcgg ttcaaggacg gccgcgtgtt cgagcgctac tcccggccgc
      961 aacgcgtcgc gttcgcgatc gtgggacgcg tgtggagctt ccgcgacgtc accgagcgcg
     1021 agcggctgct cacggccaag tcgctgctcg ccgatgcggg gcgcctgctc acgtcgctcg
     1081 acccgcgtag cgcgctcgat gcggtggtca ggctcgccgt gcccggtctc ggagatgcgg
     1141 gcgcgatcga gctgttcggc gaacccaaga tcgccgcgtc gtactcgatc gatccagcgc
     1201 agtcgttctc gccggcgtgg ccggcgggtg tcctcgccgg aagctcggcg atgtccgacc
     1261 gcgatggggt ctccgagctc gcggtgccgc tcgtgatcca ggacgaggcc atcggcgcgg
     1321 tcgcgttcgc agcgccctcg agccggcgcc acacgctcgc cgacctcgac ctcgcggagc
     1381 gcctcgcgca cgggatcgcg ctgtcgatcg agaacgcgtg gctgtacgac cacgctcggc
     1441 aggcgctgcg cgcgcgggag gagttcctcg cgatcgccgc acacgagctg cgcgggccgc
     1501 tgtcgacgat gaacctcgcc gtgcagacgc tgaagcggcg cgacgttcca caacccaccc
     1561 gtgatcggat gctctcgctg atcgagcgcg aggaccgcag gctgacccgg ttcgccgacg
     1621 acatcaccga gatcgcgagg attcgcgccg gcgcgttgca cttcgacgac gacgaggtcg
     1681 acctcgtcca gatcgcccgg gaggtcgtgg cgcggctcgc gccggacgcc gaacgctcgg
     1741 gctccacggt gttgatcgac gcaccgcatc ccgtggtcgg tcgctgggat cgcatgcggc
     1801 tcgatcaggt ggtcacgaac ctggtgatca acgcgttcaa gttcgggctc ggccggccga
     1861 tcgagctctc ggtccgcgcc cgcgacggct gggcgaccct caaggtgagc gaccagggcc
     1921 tcggtgtacg cgcatcccag cgcgaggcga tcttccggcc gttcgagcgc gcggtcccgg
     1981 tgcgccacta cggtggcctc ggcctgggcc tgttcatcgt acggacgatc gtcacccggt
     2041 acggaggcac ggtgcatgtc gagccgcgcg acagcggggg ctcgtgcttc gtggtgcggc
     2101 tcccgcaggc gggggtcgca tgatcgacgg acgtcgcgcg gtgctcgtcg tcgatgacga
     2161 tcccgacatc ctcgagatcg tcaccgtcgt gctcggcctg tacgacgtac cggcggtgac
     2221 ggcgtgcgac gggatcgagg cgctccatgc gctgcgcggg tccgccgagc tcgggctcgt
     2281 gctcctcgat ctgatgatgc cgcggatgaa cggcgtcgac gtgctcgccg agatgaagcg
     2341 cgatccggcg ctcgccgcca tgccggtgat cgtgatctcg ggaaactacc acaccgagca
     2401 ggacgtgctc gcgatgggcg ccgacgagta cctgctgaaa ccgctcgacg tcgacgtgct
     2461 gatgcgcgtc gtgtcgcggt tcgtgccagt caccggcctg cggccggagc cagaccatgg
     2521 tcacggtcag tgacccgcgc aggcgagccc gcttggccac atccacgcac gacgcgctgc
     2581 cagatccgag gccttcgctg tgcccacctg ggcaaccgca ttcctccggt gatcgagtgt
     2641 tgcccgtgcg tcaccggcgc gcaccgaagc gctcgtgccc gtcatcgtgg tcgcacaaga
     2701 ccgtgcgctt gtcctcgatc gttgccgcgg cagacttgag aacgagcatg cgataggaag
     2761 gttgccttcg aacgatgccc tcggtacgcc tcaccagaga aggcagtcga tacgacgtgc
     2821 gtcgcgaccg gcgtcagggt cgctccgtga tcatcaagcg ccagaaggat cccatggacg
     2881 aacgggcgtc cgcattgctg cgccacgagt atcatacctt gcagagcatc gagactcccg
     2941 gtgtggtcaa gccgctggcg ctcgacgagc tgaacggccg gcctgcgctg atcctcgagg
     3001 atgccgggga cgagaacctg gcggcgcacc tcacgcgggg agcgctcgat ctggatgcgt
     3061 tcctcgccct ggcggtccag atggcggcga tcgtcgcgcg cgtccaccag cgcaacatcc
     3121 tgcaccgcga tctgtgcccg gacaacttcg tcctggacga cgggcgcgtg acgctggtcg
     3181 acttcgagag cgccacgacg gcgaccgggg cggtcgcggt cgagccggtc ggcgcgctgc
     3241 tgtacatggc gccggaggcg acggggcggt tgcggcgcgg gcccgacgag cgatcggacc
     3301 tctactcgct gggcgcgatc ttctacgaga tgctcaccgg caggccgccg tttccgtccg
     3361 tcgatccggt ggagctcgtc cacgcccacc tggcgcgcaa tccggtcgcg cctgcgatcc
     3421 tcgatccctg cgtgccgcgg ccggtgtcgg acatcgcgct ccgcctgctg gccaaggatc
     3481 cggatgcgcg ctaccagacc tcggcaggcc tgctcgcgga cctcgaggag gcgcgcgcgc
     3541 agtggctgtc cgggcggacg atcccgccat tcgagctggg gcgcgaccag ctgatgcgcg
     3601 agttcccgcc accggcccga ctgtacgggc gcgagccggc ggaggccgtg ctcgccggcg
     3661 ccgtcgagcg cgtgtcgtcc ggcgccacgg agctgttcgc cctgaccggg cgggcgggcg
     3721 tcggcaagtc ggcgctcgtc gcgcagctcg ccgccaccgc gcagcgcgat ggccgctggg
     3781 tcgcggggaa gtgcgatctc ctggcaggga acctgccgta cgcgccgctg ctcgagctgt
     3841 tcggcgagct cgtgcaggag ctgctggccg cgtccgaggc cgagcgcgcc gacctgcgcg
     3901 atcggatcct gcgagcgctc gcgcccaacg cccgcgtgct gaccgacttc ctgcccgggc
     3961 tgatcccgct gctcggcgag ccgtcgccgg tccccgcgct cggtcccgcg gagacccaga
     4021 cccgctttca catggtgatg cagtcgttcg tgcgagcggt cgccgcggcg cgtccgctga
     4081 tcggcttcct cgatgacgcg cagtgggccg atgcggcgac gctgacgctc ctgcgggtgc
     4141 tcgccgagac gccccagctg cggcactgcc tgatcatcgt cgcctaccgc agcgaggatg
     4201 tcggggagca ccacgcgacc cacctggcga tcgaggcgat ccgcgccgcg ggcgtcgcgg
     4261 tccacgcgat ggagctcgct ccgctcgacg tcgccgacat cacggcgctg ctcgccgatg
     4321 cgctccggat cgcgccggaa gcggccgcgc cgctcggcga agcggtccac ggcaagaccg
     4381 ccggcaaccc gctgttcgtc cgccggttcc tgcggtacct gcacgacgcg ggtctgttcg
     4441 tgttcgatcg ccagaccggc gtgtggcagt gggatctcgc gcggatcgcc gaggcgccgg
     4501 tcaccgagaa cgtcgtcgac ctgctgatgt ccgcgatcgc gcgcctgccc ggcgagtgtc
     4561 gacgcgcgct ggcggtcgcc gcctgcatcg gcgcgcggtt cgagctcgac gtcgtggcgg
     4621 cggtatccgg agcgtcgctc gaggcgaccg gatgcgcgct cgcgcctgcg cttcgcgagc
     4681 agctgatcgt gccggtgtgg ccggcatccc ccggcggacc ggcgacctac ggcttcgcgc
     4741 acgaccgcat tcaacaggcg gtgcactcgc tcgagtccga ggacgcgcgc cagcgcactc
     4801 acctgcggat cggccgccgg ctcgccgccg cggccaccgg ctcggccgtg ccgccgttcg
     4861 cgatcgccga tcagctcaac ctcgcgatcg gcgcgatgac cgacccggcc gagcgcctcg
     4921 ggctcgccga gctcgacctg cgcgcgggca ccgcggcgaa ggcgcgctcg gcccacggcc
     4981 cggcgctggc ctatctgacg cacggcctcg agctgttgcc ggagcgggcg tgggcgacgc
     5041 accacgagct gtggtaccgg ctgcaccgcg aggcggtcga atgcgccggg ctcgacggaa
     5101 agcacgcgcg cgccgatgcg ctggtcgagg cggcgctgcc gcacaccggg tcgctcgagg
     5161 agaaggccga gctctacgca ctgcagatca tgggcgccac gctcggcgga tcgcttccgc
     5221 acgcgctgcg cgtcctgggc gacggcctgc tggcgttcgg cgtgacggtc ccgcgcgacg
     5281 acctgccggc ggccgcgatc gccgagctcg ccgcggcgcg ggatgcactc accgaaagcg
     5341 cggcggactg gctgctccag gctccgccga tgaccggcga cgagctgtcc gtggtgcgca
     5401 tcctcgcgag cgcgctgccg gcgggcagct acgtggaccc cgatctgctc gcgtggctgt
     5461 cggcccgcct ggtgagctcg acgctccgcc acggtcccgc accccagacc tcgctgggcc
     5521 tcggcatgtt cgcgttcgcg ctgctcaacg tgcacgacta ccggatggcc gagcgggcgg
     5581 gccagctcgc gatccgcgtg gcggagcggc tgcgcgatcc ggtggccacc agccgcgccc
     5641 ggttcatgta tgcggggctg atgagcttct ggctccagcc gatccgcaac agcatcccgc
     5701 tgctccagcg cgccggcggc gagctgctcg cgctcggcga tcaccagttc gcgccgtgga
     5761 acagtcagga cctggcgagc ttgctgctgg cgcagggcag cccgctcgac gaggtgctgg
     5821 ccgaggtcga tcacggcctc gcgctcgcga agcgattcga tcacgcaccg gtccggaggt
     5881 tctttgccct gttccggacc gggctgcgcg atctggtcgg aaccgcccgc gggcaggacg
     5941 ccgacctgct cgcgacggcg agcccgctca acgcgggcct gtatcacatc ctgcagctcc
     6001 agaccgccta catcctcggt gacctcgccg cggccgacac cgccgcgcgc gcggccgccg
     6061 agcagctgcc atttttggcc ggggtgttct tctggatcgt cgactacgcg ctgtacgacg
     6121 cgctgaccgc ggcggcccgg ctggactcgg cgggcgccga cgagcgcgcg gcgctgcgcg
     6181 cacggatccg cgatcacctg cgggagctcg aagcgtgggc cgagctgtgc ccggacaacg
     6241 tcctccacaa ggtccggctg ctggcaggcg agatcgcggc catcgatcac cagccgctcg
     6301 acgcggcgcg cctgtacgag gaagcgatcg atcgggccgg ccgcgagggc ttcgtccacg
     6361 acgcggcgct cgcgcacgag cggtgcggcc ggatgtacct gcgcgtcggg ttgaagcgcg
     6421 aggcggaggc ccacctggcg gacgcgatc
//
LOCUS       DASVOR010002519         1628 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_406909,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002519 DASVOR010000000
VERSION     DASVOR010002519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1628)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1628)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1628
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406909"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1116)
                     /locus_tag="VF516_46235"
     CDS             complement(<1..1116)
                     /locus_tag="VF516_46235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEX8115237.1"
                     /translation="MTSYLDDNRGILSWLATRDHKRIGIMFLIATAGALLLGGGFALA
                     LRIELLMPGKLYLSALDYNRMFTHHGVVMVFLFMVPVIPTVFGNFFLPIMLGAPDLAF
                     PRLNLASFYVYVAGALIVIAAMWFGGIDTGWTFYAPYSTTSPTAVATGTFGVFVVGVS
                     SILTGVNFIATVHTMRAKNMGWTQMPLFVWGIYATAIIQVLATPVLGMSLLLVAVDHA
                     WSLGIFDPNMGGDPVLFQHLFWFYSHPAVYIMILPAMGVVSEVVCTFSHNPPASYRAI
                     AVSSLGIAFIGFLTWGHHMFVAGMSLFDVGAFGILSMFVAIFSAIKVFTWTATLRHGA
                     VRVTTPMLYFFGFMFLFVFGGMTGVALATSSLDIHWHD"
     gene            complement(1097..>1628)
                     /locus_tag="VF516_46240"
     CDS             complement(1097..>1628)
                     /locus_tag="VF516_46240"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEX8115238.1"
                     /translation="PAVVGEESPSPPLSLAAVGRSVAADAGCLRCHTVDGTPHIGPTW
                     AGLYSATIPLQGGGRVIADEQYLTRSMMDPAADIHLGFPNVMPSYQGLLTAPQVGAIV
                     EYIRALRDVSRHVGNQPLPATVLPPAPIVTPLPGDPPRALSPDDLLRQAHPGVPLYPP
                     PSEPPPSEPNHDQLPR"
BASE COUNT          309 a          527 c          581 g          211 t
ORIGIN      
        1 gtcgtgccag tggatgtcga gcgacgaggt cgcgagcgcg acgccggtca tcccgccgaa
       61 cacgaacagg aacatgaagc cgaagaagta cagcatcggc gtggtgaccc ggaccgcgcc
      121 gtggcgcagc gtcgcggtcc acgtgaacac cttgatcgcc gagaagatcg ccacgaacat
      181 cgacaggatc ccgaacgcgc cgacgtcgaa cagcgacatc cccgccacga acatgtggtg
      241 accccaggtc agaaagccga tgaacgcgat gccgagcgac gacaccgcga tcgcccggta
      301 cgacgccggc gggttgtggc tgaacgtgca caccacctcg ctgaccacgc ccatcgccgg
      361 caggatcatg atgtacaccg cggggtggga atagaaccag aacaggtgct ggaacagcac
      421 cggatcgccg cccatgttgg gatcgaagat gccgagcgac cacgcgtgat cgaccgcgac
      481 cagcagcagc gacatgccga gcaccggcgt cgcgaggacc tggatgatcg cggtcgcgta
      541 gatcccccac acgaacagcg gcatctgcgt ccaccccatg ttcttggccc gcatcgtgtg
      601 gacggtcgcg atgaagttga ccccggtcag gatcgacgat acgccgacga cgaacacgcc
      661 gaaggtgccg gtcgccaccg cggtcggcga ggtcgtgctg tacggcgcgt agaacgtcca
      721 gccggtgtcg atgccgccga accacatcgc cgcgatcacg atgagcgcgc cggcgacgta
      781 gacgtagaag ctcgccaggt tgagccgcgg gaacgcgagg tccggcgcgc cgagcatgat
      841 cggcaggaag aagttgccga acacggtcgg gatcaccggc accatgaaca ggaacaccat
      901 caccacgccg tggtgggtga acatccggtt gtagtccagc gccgacaggt acagcttgcc
      961 gggcatcaag agctcgatcc ggagcgcgag cgcgaacccg ccgccgagca gcagcgcgcc
     1021 ggcggtcgcg atcaggaaca tgatcccgat gcgcttgtga tcgcgggtcg cgagccacga
     1081 caggatcccg cggttgtcat cgaggtagct ggtcatggtt cggctcggag ggtggtggct
     1141 cggagggtgg tgggtagagc gggacgccgg ggtgggcctg gcgcaggagg tcgtcgggag
     1201 acagcgcgcg cggcggatcg ccgggcaacg gcgtgacgat cggcgccggg ggcaatacgg
     1261 tcgccggcag cggctggttg cccacatggc ggctgacgtc gcgcagcgcc cggatgtact
     1321 cgacgatcgc gccgacctgc ggcgcggtca gcaggccctg atagctcggc atcacgttgg
     1381 ggaatccgag gtggatgtcg gccgccggat ccatcatcga tctggtcagg tactgctcgt
     1441 cggcgatcac ccgcccgccg ccctgcagcg ggatcgtcgc gctgtacagc ccggcccagg
     1501 tcgggccgat gtgcggcgtg ccgtcgacgg tgtggcagcg cagacagccg gcgtcggcgg
     1561 cgaccgaccg gccgaccgcg gcgagcgaca gcggcggcga cggcgactcc tcgccgacca
     1621 ccgccgga
//
LOCUS       DASVOR010002520        14502 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_320524,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002520 DASVOR010000000
VERSION     DASVOR010002520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 14502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14502
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320524"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VF516_46245"
     CDS             complement(<1..432)
                     /locus_tag="VF516_46245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115239.1"
                     /translation="MARWNNANARLRHSRSPGHGAPFQIAHPSWDRGRTRSALVPGSS
                     PQDRLAHPGLRVMVRQQHRRGSMANTELRGGGVQQDSLLERVTHQLTTTARGDRVART
                     IGWTAIALGASHLLMPSAVARWLAPGGRGALVRLLGAAELAS"
     gene            852..1739
                     /locus_tag="VF516_46250"
     CDS             852..1739
                     /locus_tag="VF516_46250"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEX8115240.1"
                     /translation="MPMEHPEPSVEPTPVDDEQDRDLQDLFGDIERSGVLPAHVPSYQ
                     AADAVLCALEMRLPRTQANEIVCDVPPTLSLLVRRCVIHQTDRPELSFDKPAFLRLIA
                     SALQISVAEAQRVTRAVFVAVQKRLPEDEILEVRRRLPRELNALWLDQEPPPPEDTGA
                     VAGGWPVEEPTETILREIEQSGVLPKGRSATEALQAVLCTLSLELTGHEAQELAASSR
                     TLHRLLGPCVSHRGERPEVMRRQTFLHRLAEHLGVDEEWAEQVARTVFAAVRGRLPPG
                     AIARIDGRLPSSVRLLWIP"
     gene            2261..2842
                     /locus_tag="VF516_46255"
     CDS             2261..2842
                     /locus_tag="VF516_46255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115241.1"
                     /translation="MTKIALPCILALAFIAIPTTAAAQSIVTVRCTTDSLDATAALQR
                     IEWARRCALLTNTGGPNSWFFSTLAADDVTFVGAKDYREIDPNHAYSGSVNDYNVNYS
                     YSFCRYPSSSIFSVIQETSGPTAGFWKWSRPVQRPRVFYPTFDTTMTGTGTQLFPLPT
                     LPGECKLYQRNPTTGGFSRWTGNFYVLAYCVST"
     gene            3009..4136
                     /locus_tag="VF516_46260"
     CDS             3009..4136
                     /locus_tag="VF516_46260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Hint domain-containing protein"
                     /protein_id="HEX8115242.1"
                     /translation="MYKPLRLIFAIGLAAAPTTAWAQVQARCGSDSLDPTQAAARLEW
                     ARKCGLLTNTNGPGDWQPSQTSIDTSFNAPQEYIEHDTTRAFSGNADAYTVNYQFARN
                     LYPISPFYNATLEASGPTSGYWKWQAFARVNNVPVAQSPRPRPLYPTFGPLNDTVTPW
                     FPHPSLLSSPSSWPSIGCELYTDGTGKTRAVGAFWVVSYCESSCYTPDQSLRFSDGDV
                     NIVEALQARRNDLVTLSPDATLDAITTQVDKVFSYTVEIRDTTNPIVKVATASGGSLS
                     VTTEHPVLTSKGRLVKAETLTVHDELLLADGTPDPITHVERSTHFGKVYNIKPLSPEP
                     VSNILIAQGFLVGSARFQNDDVGYMNRILLFRAVPHGVIPK"
     gene            4383..4931
                     /locus_tag="VF516_46265"
     CDS             4383..4931
                     /locus_tag="VF516_46265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115243.1"
                     /translation="MPDALIARILEQDEKLALFMNYHKTVLLDTQRRDEYHKLLSDPD
                     MMAAMAEGLTTPGSGEVSQEEYYHRLMQVDYFAAALTWKDNPQRQEVISLTGELIAKD
                     NFTGDQNDARRQMLGGLKMELYRLLYEQDAGKTQQLVAQAKGTRMEPLVRWMAEEELR
                     RRTLEEKEKLRQDSAEQQAKAR"
     gene            5337..6878
                     /locus_tag="VF516_46270"
     CDS             5337..6878
                     /locus_tag="VF516_46270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115244.1"
                     /translation="MVPLADLFALSGDGFVTRSECVQGCNYTWRDLEGTERTRRENLF
                     SVTGTVVSPDGRRALLVALEELENCTFDQFQVPVARGTLQLLELASGASSFELRLRSN
                     IWNAPAFTPLNDWFIAAPNQGTDCSGALGLRSTSSPFEPPSGFDPTLQLVAAVDAQRW
                     LALRNFDLGLIDPTVPNNFQFLAEDPSRFDVSHGWVHVYIGFALLAQDVLSLPPTGGM
                     LETTLRDQDWLPFGVQGRWIRVCEALQTTDDHKCRVVDAQAEVAPVDFHVTMAADHRD
                     DAVLLIDGAVVYVGPLPDGSRAVQRVQFATGRHEILHPGNGTLQPLGDGTAALLLQDG
                     AAWLIDAEHDELVAEHVRHVVSTPQLQIPGRGPARQDDVAALVLSPDAVRTTLVVLDA
                     RTRRIATLTDSLFFTPPPDGGFAFDDGCGQPWTTHHAGSVLEGLFQQARHLFFVEQGA
                     QGEPATIWLIPIDLSAPPRRLAELAGAEASCHVPFTSPDGDRVGFAEDAPDGTTTRIT
                     LSPER"
     gene            7031..7609
                     /locus_tag="VF516_46275"
     CDS             7031..7609
                     /locus_tag="VF516_46275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115245.1"
                     /translation="MAKSLFLFILNVAIIAVPTAVLAQSTPPRCSTDALDPDLAVQRL
                     EWARMCGLTRNTGGVNSAFDSTAAFDLMFNPAKEYREIDPNHAFSGNTEDYNVNLNYS
                     LARYTATPTYTVTQESTGLTTGYWKWSHTTQRAQPKYPTFETSPVAGSGTQLIPLPSF
                     FNDCNLYVWDRTAGTLTPWTGSFYVVAYCPST"
     gene            complement(7899..9716)
                     /locus_tag="VF516_46280"
     CDS             complement(7899..9716)
                     /locus_tag="VF516_46280"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEX8115246.1"
                     /translation="MSSRSLVSFLVVAACGGKPPPPAPAPVAQAPAPPAAAAPDLATR
                     RKQLADLIEERWQYQLKTHPEMASRLGDHRYDDRWSDHSPEGFAAENAEQKEMLARFS
                     AVDTTGFPEQEALNKELIVRILQQSVDTMKFEAWLMPVTQISGVHLWLPELVSMLRFE
                     SVEDYEHYLARLHAIPKVFERTIALMKLGATKHLMPPKILLDKVVPQARGLGTGKLTD
                     NPFAQPVAKFPAAIAKADRDRLRAAILAAVKDEVQPAYTRFATYLENDYVPQGRSEPG
                     VWALPDGDARYAADVKQSTTTELTPEQIHQTGLAEVSRIEGEEAKLATKLGFKSLDEL
                     RKHVRGDKRLYAKSRADILARYEKYTAQMYEKLPSLFGRLPKQHMQVEQVESFREETA
                     GAAEYNQGTPDGSRPGVVHVNTSDPTKRLTLDMESTAYHEGVPGHHLQLTIQQELGDM
                     PEFRKHVNYTAYAEGWALYAERLGEEVGFYQDPWSMYGHYEDEMLRAIRLVVDTGFHY
                     KRWTRDQVVRFFHDHSAIDEVNVQSETDRYIAQPAQALGYKVGQLTILRLRSEAQEAL
                     GTRFDIRAFHDELLGSGALPMDLLERRIHAWIERTRTPR"
     gene            complement(10423..11574)
                     /locus_tag="VF516_46285"
     CDS             complement(10423..11574)
                     /locus_tag="VF516_46285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115247.1"
                     /translation="MSDEPTKPSEEKRPQQMVLGLIAGPGLPEKLAAYLAERLPSALA
                     RRFHGTSWKVVVGTEPFAGAGADVDLPQLARRRMLEEGWDLVVCLTGRPLRVGRHPVV
                     AEASPALGVGVISVPAMGAIGLTRRLFKAVLLTVDAMLCTGGGDRAKARSAQRVRIED
                     LARVSSALGSSLEMEQGRIAFATATAAGNLRLLFGVIRGNQPWALIVGLSNSLVAALG
                     VSAFGLTSPVIWRIANAMSLPRLLLLTTASLLVIGFTLMTAHRLWERAPTRDARTRVL
                     LINLATSSTVAIGVVTSYIALLIFNYAFGGALIPEQVLQRELGHTVSTVHYVRIAWLV
                     SALATLGGALGTVLETNAAVRAAAYCIRPVSRANEPPPRRARTMMKRSL"
     gene            12133..12324
                     /locus_tag="VF516_46290"
     CDS             12133..12324
                     /locus_tag="VF516_46290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115248.1"
                     /translation="MATQQAVIITLDMAQQKTLGESIEEANAHVTRLLTEGWRLMWAT
                     PFSGSQTNTVALMLVIERG"
     gene            complement(12680..13102)
                     /locus_tag="VF516_46295"
     CDS             complement(12680..13102)
                     /locus_tag="VF516_46295"
                     /inference="COORDINATES: protein motif:HMM:NF020687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1931 family protein"
                     /protein_id="HEX8115249.1"
                     /translation="MFRVAAGVDIDKEDLRHYEGFLDVKVADLLRRACEVAQDSGRKV
                     ILPHDLPITKGLARCIDDFRTIDTEEQLGLAPVLEKLGKRPQFDLSYDDEVYAQLPYI
                     IGGMTVALARSFKVIDPDVVGIVTAHWLRVLQLFDLVW"
     gene            complement(13193..13489)
                     /locus_tag="VF516_46300"
                     /pseudo
     CDS             complement(13193..13489)
                     /locus_tag="VF516_46300"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(13618..14457)
                     /locus_tag="VF516_46305"
     CDS             complement(13618..14457)
                     /locus_tag="VF516_46305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEX8115250.1"
                     /translation="MASIRTVKLPSGESVPALGQGTWHLGEHPSKRQDEIAALRLGLD
                     LGMTLIDTAEMYVGAEDLIREAIGMVGRRNECFIVDKVRPSHATRRGTIQACEDSLRR
                     LATDRIDLYLLHWRGTVPLEETVDAFEELIDTGKIRYWGVSNFDHDDMTDLFQVPEIE
                     PQTDQVLYNLAHRGIEWDLLPWCRSRGIPLMAYSPVEGGTLVDHPVVRRVAKRHDATP
                     AQIAIAWVLRQPDVITIPRAGNPEHVRENRGALDIQLTSKDLRDLDDVFEPPKYKVPL
                     DVS"
BASE COUNT         2450 a         4911 c         4657 g         2484 t
ORIGIN      
        1 gctcgcgagc tccgccgcgc cgagcagccg gacgagcgcc ccgcgtccgc ccggcgccaa
       61 ccaccgcgcg accgcgctcg gcatcagcag atgcgacgcg cccagcgcga tcgcggtcca
      121 gccgatggtg cgcgcgaccc gatcgccgcg cgccgttgtc gtgagctggt gagtcacgcg
      181 ttcgaggagc gaatcctgct ggactccgcc accccgcaat tccgtattcg ccatgctgcc
      241 tctccgatgc tgttgccgaa ccatgacccg gagccccgga tgcgcaagcc ggtcttgcgg
      301 cgagctgccg gggacgagcg cggagcgcgt gcgcccgcga tcccagctcg ggtgtgcgat
      361 ctggaatggt gcgccgtggc ccggcgaccg agagtggcgc aggcgcgcat tggcattgtt
      421 ccatctggcc atcccgcgag cgttctggca cgtctgagtc ccgtgcggtg actcgggagc
      481 ggatgacggc gaagcacgct gttcgatgcc tcgacgacga tccccgacga gccacgcggg
      541 aggctcgcgg tcgcgggtgc caaacttgca gctcgagtca tgaaacaaaa caaggccaaa
      601 gaaggagtga tcgtgaactg ctcggggatc gcgctgttca tcgcgaaggc agacgactcc
      661 acgcgaagcg acggacgttc gttacgtggc tcggtttgcg gcgccatgcg ggaggttacc
      721 gccgcggact gccttcgtca gcgcgcccgc aacttcactg acagtgcaag cccgacgtgc
      781 gagtccggcc gacagcgtaa caaacgacgc tggagccggg cactccgctt gcttcgacct
      841 gatcgcgagg gatgccgatg gaacacccag aacccagcgt cgagccgacg cccgtcgacg
      901 acgaacaaga ccgtgacctc caggacctgt ttggcgacat cgagcgcagc ggggtgttgc
      961 ccgcgcacgt gccttcatac caggcggccg atgcggtcct gtgcgcgctc gagatgaggc
     1021 taccccgcac gcaggcaaac gagatcgttt gcgacgtgcc gccaacactc agcctgctcg
     1081 tccgccgatg cgtcatccac cagaccgaca ggccggagct ctcgttcgac aagcccgcgt
     1141 tcctgcgtct gatcgcgagc gcgctgcaga tctcggtcgc agaggcgcag cgagtgacgc
     1201 gcgccgtgtt cgtcgcggtg cagaagcgcc tgcccgagga cgagatcctg gaggtccggc
     1261 gcaggcttcc gcgcgagctg aacgcactct ggttggatca ggaacctccg cctcccgagg
     1321 acacgggcgc ggtcgcgggc ggctggcccg tcgaggagcc gaccgagacg atcctccgcg
     1381 agatcgagca gagtggcgtg ctgcccaaag gacgctccgc gaccgaggcg ttgcaggccg
     1441 ttctctgcac cctttcgctg gagctgacgg gccacgaagc gcaggagctc gccgcttcct
     1501 cgagaacctt gcatcgcctc ctcggcccat gcgtgagcca tcgcggtgaa cggcccgaag
     1561 tcatgcgccg gcaaacattc ctgcatcggc tcgccgagca cctcggggtg gacgaggaat
     1621 gggcggaaca agttgcgcgc acggtgttcg cagcggtacg tggccggctt ccgcccggcg
     1681 cgatcgccag gatcgacggc cggctcccga gcagcgtgcg gttgctgtgg attccgtgat
     1741 cgcggtcgct gcgcgccgcg tattcgtggg cttgcacaaa actaaaatcc atctgtcggt
     1801 gccagcgtca tggcgctcgc gacgacttcg cgtgttgatc tcgtcgcagg gcggatcgtc
     1861 gtccggatcc tccggatctg ccgccgagca gggcgagctc tggttcgacg acgcttcgta
     1921 gcccgcacga ccgccgacgc gctcggcagg ggacggatct gcgcggacgt cgacccaatg
     1981 tgcgcccgct gacacagccg gcatctggtg gtgcccgccg gctcgccggt ccccacctcg
     2041 gggcacatcg aggcatcccg ggggagcgcc cgaaaccccg cttgaatgtc cttgccgctc
     2101 gaatcacgac acctaggaca tgccgcctta agccgcagca gacaatcctt gaccaatgtc
     2161 tttataagaa ggattcttag ggcgtggtaa gttttcagtg gcagtttaat gtaaccctga
     2221 tcacacaaaa cttggtctcc ggacaggtga ggggagaacc atgaccaaga tcgcgcttcc
     2281 gtgcattctc gccctcgcct tcatcgcgat tcccaccacc gccgcggcac aatccatcgt
     2341 aacggttcgt tgcaccacgg acagcctcga cgcgactgcg gcgctccagc gcatcgagtg
     2401 ggcgcgcagg tgcgcgctcc tcaccaacac cggcggtccg aacagctggt tcttctcgac
     2461 ccttgccgcg gatgacgtga cgttcgtcgg ggccaaggac tatcgggaga tcgaccccaa
     2521 ccacgcctac tccggaagtg tcaacgacta taacgtcaac tactcctaca gcttctgccg
     2581 ctacccgtcc tcgtcgatct tcagcgtgat ccaggaaaca tccggtccca ccgctggctt
     2641 ctggaagtgg tcacgccccg tccagcgccc gcgtgtcttc tacccgacct tcgacaccac
     2701 gatgaccggc accggcactc agctgttccc gctccccacc cttcccggcg aatgcaagct
     2761 ctaccagagg aacccgacca caggcgggtt ctcgcgctgg acgggcaact tctacgtgct
     2821 cgcatactgc gtgtcgacgt gattcgatcg tcacgctgcc ttcatcgatc gaaccggtcc
     2881 agatcatgta atccacgggt cgccttcgct accggccctg ctctgcatct cttcgagctc
     2941 ctcgagctcc tctgaggcag gataacaaga caatcgaagc atccagccaa tcagccgcaa
     3001 aggaatccat gtacaaacct ctcagactca tttttgccat cggcctcgcc gcagcgccaa
     3061 cgaccgcctg ggcacaggta caggcacgct gcgggtccga tagcctcgac ccaacccagg
     3121 cggcagcgcg tctcgagtgg gcccggaagt gcggcctcct caccaacacg aacggtcccg
     3181 gcgactggca gcccagtcaa acgtcgatcg atacatcgtt taacgcgcct caggaatata
     3241 tcgagcacga cacgactcgc gccttcagcg gcaatgccga tgcctacacg gtgaactatc
     3301 agtttgcccg caacctgtac ccgatctcac cgttctacaa tgcaaccctc gaggcgagtg
     3361 gaccgacctc ggggtactgg aaatggcagg cgttcgcaag agtaaacaat gtgccggtag
     3421 cgcagtcacc tcgcccgcgt ccgctgtatc cgaccttcgg cccgctcaac gacaccgtca
     3481 caccgtggtt tccgcacccg agcctgctct catcgccatc gtcgtggccc tcgatcgggt
     3541 gcgagctcta cacggatgga accggcaaga cgcgggcagt gggcgcattc tgggtcgtct
     3601 cttactgcga gtcgagctgc tacacgcccg accagagcct gcgattcagc gatggtgacg
     3661 tgaacatcgt cgaggcgctg caggccagac gcaacgatct ggtcacgcta tcgccggacg
     3721 cgacgctcga cgccatcacc acgcaggtcg acaaggtgtt cagctacacc gtcgagatcc
     3781 gcgacaccac gaaccccatt gtcaaggtcg ccaccgcgtc gggcggctcg ctgagcgtca
     3841 cgaccgagca cccggtgctc accagcaaag gccgcctggt caaggccgag accctgaccg
     3901 tgcacgacga gctcctcctg gccgatggca cccccgatcc gatcacccac gtcgagcgaa
     3961 gcacccactt cggcaaggtc tacaacatca agccgctgtc gcccgagccg gtctccaaca
     4021 tcctcatcgc gcagggcttc ctggtcggct cggcccggtt ccagaacgat gacgtcggct
     4081 acatgaaccg catcttgctc ttccgtgcgg tcccccacgg cgtcattccc aagtaatcgc
     4141 cattcgttcg tccgaggaga gcgttcatga agaactcgtc gccgaccacc cgactcgcgc
     4201 tcggtggggt cgtggtggtc atcgcggtcg cgctcctgtg gttgaccccg gcccagagcc
     4261 cccagggccc caccggctca ccgggtggag agcgaccgcg ggcggtgagc actgccaggg
     4321 tccagcgtac gccaagtgac tcgctcccga acgagccgcc gcgcatgatc gtggcgccac
     4381 cgatgccaga cgcgctgatc gcgcgcatcc tcgagcagga cgagaagctc gcgttgttta
     4441 tgaactacca caagacggtc ttgctcgata cgcagcggcg cgacgagtac cacaagctgc
     4501 tgtcggatcc cgacatgatg gccgcgatgg cagagggtct gacgacgccg ggtagcggtg
     4561 aggtcagcca ggaagagtac taccaccggc tgatgcaggt cgactacttc gcagccgcgc
     4621 tcacctggaa ggacaacccg cagcggcagg aggtgatcag cctgaccggc gagctcatcg
     4681 ccaaggacaa cttcacgggc gatcagaacg atgctcggcg ccagatgctc gggggcctga
     4741 agatggagct ctaccgcttg ttgtacgagc aggacgcggg gaagacgcag cagctggtgg
     4801 cacaggccaa gggaacccgc atggagccgc tggtccgctg gatggccgag gaggagctcc
     4861 ggcggcgtac gctcgaagaa aaggaaaagc tccggcagga ctcagcggag caacaggcga
     4921 aggcgcgctg agtgcaccgc cgtgccgctc cgcgtgctac aggtcgctcg gccatgggat
     4981 ctgcagccgt catggctctg tgctgctgta cactgaagtc gcccgcggcg cccggccccc
     5041 ggcgccgcgg aggtcgacag tgggggcacg agcctcagag accgcgggtc gctctcgtca
     5101 accagtcgaa caagcaagtg tcgacacttc agtacctttg ccttcacgtg ctcgagctcg
     5161 tgagcctcgt gctcgtcgcc gcgtgcggat ccggctcgcc cgcgctaccc gatgcgggca
     5221 ctcccctcga cgcggctccc ggtgtaccgc ccgatggctc acctggtgag accgattcat
     5281 ggacacgtgt cgactggcgc accacgcgcg acactccctt accgacgacc actgccgtgg
     5341 tcccgctggc cgatctcttt gcgctgtccg gggacggctt cgtgacgcgg tcggagtgcg
     5401 tgcaaggctg caactacacg tggcgtgacc tcgaggggac cgagcgcacg cggcgcgaga
     5461 acctgttcag cgtgacgggc accgtcgtgt cacccgatgg cagacgagcg ctgctggtcg
     5521 cgctcgagga gctcgagaac tgtaccttcg atcagttcca ggtgccggtc gcgcgcggaa
     5581 cgctgcagct cctcgagctt gcctccggcg cgagcagctt cgagctcagg cttcgcagca
     5641 atatctggaa tgcgcccgca ttcacgccgc tcaacgactg gttcatcgcc gcgcccaacc
     5701 agggcacgga ttgctccggc gccctcgggc tacgatccac gagctcaccg ttcgagccgc
     5761 cgagcgggtt cgatccgacg ttgcagctcg tcgcagcggt cgacgcgcag cgctggctcg
     5821 ccctccgcaa cttcgacctc gggctcatcg atccgacggt cccgaacaac ttccagttcc
     5881 tcgccgagga cccgtcccgg ttcgatgtct cccatggctg ggtccatgtg tatatcggat
     5941 ttgctctcct ggcccaggac gtgctttcgc tgccgccgac cggcgggatg ctggagacca
     6001 cgctgcgcga ccaggactgg cttccgttcg gcgtacaggg ccgctggatc cgggtctgtg
     6061 aggccctcca gactacggat gaccacaagt gccgcgtggt cgacgcgcag gcggaggtgg
     6121 cgccggtcga cttccacgtc accatggcgg cggatcatcg cgacgacgcc gtcctgctca
     6181 tcgacggcgc tgtcgtgtac gtcggtccgc tacccgacgg cagccgcgcg gtacagcgag
     6241 tccagttcgc caccggccgg cacgagatcc tccatcctgg aaacggcacg ttgcagccgc
     6301 tcggcgatgg cacggccgcg ctgctgctgc aggatggtgc cgcgtggctg atcgacgccg
     6361 agcacgatga gctcgtggcc gagcatgtgc gccacgtggt gtccacgccg cagctgcaga
     6421 ttcccgggcg cggtccggcg agacaggatg acgtggcggc cctggtgttg tcgcccgatg
     6481 ctgtccggac cacgctcgtg gttctcgacg cgcgcacccg gcgcatcgcc accttgaccg
     6541 acagcttgtt cttcacaccg ccccccgacg gcgggttcgc cttcgatgac ggttgcgggc
     6601 agccctggac gacgcatcat gccggcagtg tcctcgaggg gctgttccag caggcgcggc
     6661 acctgttctt tgtcgagcag ggcgcgcaag gcgaaccggc gacgatctgg ctgatcccga
     6721 tcgacctctc ggcaccgcct cgccgcctcg ccgagctcgc cggagctgag gcgtcgtgtc
     6781 acgtaccgtt caccagcccg gatggcgacc gggtcggctt cgccgaggat gccccggacg
     6841 gaacgaccac ccggatcacg ctgtccccgg agaggtgagc acgaccggtc cgccgcaagt
     6901 actccatggc ctgcgggtgc tcgttcgctg gctccgccct gcacagcggc gacgcgcctt
     6961 gctcgcgatc atccgatcgt cctccagacc ccctcgcagc ttgcagttcc ctccatcctc
     7021 cgagacaacc atggcgaaat ccctattcct gttcattctc aacgttgcca tcatcgccgt
     7081 tcccacagcg gttctggcac agtccacgcc cccgcgctgc tccacggatg ccctcgatcc
     7141 ggacctggcg gtccagcgcc tcgagtgggc ccgcatgtgt ggcctcaccc ggaacacggg
     7201 cggcgtgaac agcgcgttcg atagcacagc ggccttcgat ctgatgttca atccggccaa
     7261 agaatacagg gagatcgatc ccaatcacgc cttcagtggg aacaccgagg actacaacgt
     7321 caacctcaac tacagcctgg ctcgctacac ggccaccccg acgtacacgg tgacccagga
     7381 gtccaccggc ctcaccaccg gctactggaa gtggtcgcac accacccagc gcgcccagcc
     7441 gaagtacccg acgttcgaga cctcgccggt ggccggcagc ggcacccagc tcatcccact
     7501 cccctcgttc ttcaacgact gcaacctcta cgtgtgggac cggaccgcgg gcacgctcac
     7561 gccgtggacg ggcagcttct acgtcgtcgc ctactgcccg agcacctgat cgacaaggtg
     7621 accaccgctg ctcgctgagc gtcaccagcg agccagatcc tcctctacat gacacgccga
     7681 ggttggcgtc gccggtcagc gtgcgcatca attgctgagc gacaactaat ttagttgacg
     7741 tcgtccagcc gtggagcgcg tgggcgccgt gctcgcggtg gcggtggtcg cgaccgggct
     7801 cgccggcgac ctcggcccga cctcggccgc cgcggcgagc gccgcgctgt gcgatccgcg
     7861 ggttcggtcg cggaagtccg cgcctggcta cgcaagcatc agcgcggcgt cctggtgcgc
     7921 tcgatccagg cgtggatccg gcgctcgagc aggtccatcg gtagcgcgcc cgagccgagg
     7981 agctcgtcgt ggaacgcgcg gatgtcgaat ctggtgccga gcgcctcctg cgcttcgctg
     8041 cgcaggcgca ggatcgtcaa ctggccgacc ttgtagccga gcgcctgcgc cggctgcgcg
     8101 atgtaccgat cggtctccga ctgcacgttg acctcgtcga tcgccgagtg gtcgtggaag
     8161 aacctgacga cctgatcgcg cgtccatcgc ttgtagtgga agccggtgtc gacgacgagg
     8221 cggatcgcgc gcagcatctc gtcctcgtag tggccgtaca tgctccacgg atcctgatag
     8281 aagccgactt cctcgccgag ccgctccgcg tacagcgccc agccctcggc gtacgcggtg
     8341 tagttgacgt gcttgcggaa ctccggcatg tcgcccagct cctgctggat cgtgagctgg
     8401 aggtgatggc cggggacgcc ctcgtggtag gcggtcgatt ccatgtcgag cgtgaggcgc
     8461 ttggtgggat cggaggtgtt gacgtggacg actccgggcc gcgaaccgtc gggcgtgccc
     8521 tggttgtact cggcggcccc ggccgtctcc tcgcggaagc tctcgacctg ctcgacctgc
     8581 atgtgctgct tcgggaggcg gccgaacagc gacggtagct tctcgtacat ctgcgcggtg
     8641 tacttctcgt agcgggcgag gatgtcggcg cggctcttcg cgtacagcct cttgtcgcca
     8701 cggacgtgct tgcgcagctc gtcgaggctc ttgaagccga gcttggtggc gagcttggcc
     8761 tcctcgccct cgatccggct gacctcggca aggccggtct ggtggatctg ctcgggcgtc
     8821 agctcggtcg tggtcgactg cttgacgtcg gccgcgtagc gcgcgtcgcc gtcgggcagc
     8881 gcccacacgc cgggctcgct gcggccttgc gggacgtagt cgttctcgag gtaggtcgcg
     8941 aagcgcgtgt atgccggctg gacctcgtcc ttgaccgccg ccaggatcgc agcgcgcagc
     9001 cgatctcgat cggccttcgc gatcgccgcc gggaacttcg cgaccggctg cgcgaacggg
     9061 ttgtcggtga gcttgccggt gccgaggccg cgggcctgcg ggacgacctt gtcgagcaag
     9121 atcttcggcg gcatcaggtg cttcgtcgcg ccgagcttca tgagcgcgat cgtccgctcg
     9181 aagactttcg ggatcgcgtg gaggcgcgcg agatagtgct cgtagtcctc gacgctctcg
     9241 aaccgcagca tcgagaccag ctccggcagc cacaggtgca cgccgctgat ctgtgtgacc
     9301 ggcatcaacc aggcctcgaa cttcatcgtg tcgaccgact gctggaggat ccggacgatc
     9361 agctctttgt tgagcgcttc ctgttccggg aagccggtgg tgtcgaccgc cgagaaccgg
     9421 gcgagcatct ccttctgctc cgcgttttcg gccgcgaatc cttcgggcga gtgatcggac
     9481 cagcggtcgt cgtaacggtg atcgccgagc ctgctcgcca tctcgggatg ggtcttgagc
     9541 tggtactgcc agcgctcttc gatcagatcc gcgagctgct tgcgccgcgt cgcgagatcc
     9601 ggcgctgctg cggcgggcgg agcaggtgcc tgcgcgacgg gcgccggcgc cggcggcggc
     9661 ggcttgccac cgcaggccgc aacgacgagg aagctgacga gcgagcgcga gctcatgcgg
     9721 cgaccctagc accgtgttcg ccggcgcctc gcaaccgttt gtggtacgcg gtgcgcttgt
     9781 gatccgggtg gagtagcctt gccattgccc gtcggcccgg tcgccagtac acccgcaccg
     9841 cgcgaggcag gaggatcacg acgccgacct cgccagctcg cgcatgagtg cggcgatcca
     9901 ggtcggagac gcgccctcca gctcgctctc gatcccatcg ggcgtccgga tcattgccgt
     9961 cggcccgacg gggggcagcg ccggtcacca cggccgggac gaaggtcagc gccgtgctcg
    10021 tctcgggctg cgcggcgtgc agttgcttcg tccaccaggc gagccgctgc gggaccagcc
    10081 cgcgcgaccg agcgaatgcc tcgaacggga accggaacca tcaaaaagcg cgaccgccgc
    10141 gccatcaaaa agcgcgctca cgctcacggg ctaccacgcc ggactgcgcg cggccgagca
    10201 cgccaggtgc cgcagcccca gcacgagctg cacgttcgcc tgatcgcgag cagcctcccc
    10261 gaggacctcg cggagttgcg gacgggtggc ttccgttcgc cctgtcttcc tggcagtgtt
    10321 cttcatcggc ggttccttcc ggtaagcgcc ccgagaatcc tctcggggtc ggttggaccg
    10381 ccacctcaat ttcaacaggg atcgggacta cgccggccgt ccctacagcg atcgcttcat
    10441 catggtccga gctcgccggg gtggcggctc gttggctctg gacaccggtc ggatgcagta
    10501 cgccgcagcg cgaacggccg cgttggtctc gaggacggtg ccgagtgcac cgccgagcgt
    10561 ggccagcgcg ctcacgagcc atgcgatgcg cacgtaatgg accgtcgaga ccgtgtgtcc
    10621 cagctcgcgt tgcaagacct gctcggggat gagcgctccg ccgaacgcgt agttgaatat
    10681 cagcaatgca atgtaggacg tcaccacgcc gatcgcgacg gtcgacgagg ttgcgagatt
    10741 gatcagaagg acccgtgtgc gcgcatcgcg ggtcggagcg cgttcccaca gcctgtgtgc
    10801 agtcatcagc gtgaacccga tcacaagcaa cgaggcggtc gtgagcaaga gcaagcgcgg
    10861 aagactcatt gcattcgcga tccgccagat caccggagac gtgagtccga aggcagacac
    10921 cccgagtgcc gcgaccaggg agttcgacag cccgacgatc aaggcccagg gctgatttcc
    10981 acgaatcacg ccgaacagga ggcgcaggtt gccggcggcg gttgctgtcg cgaacgcgat
    11041 ccttccctgt tccatctcga gcgaactgcc caaggctgac gacacccttg cgagatcctc
    11101 gatccggact cgttgcgcgg agcgcgcttt tgcacgatcg ccgccgcccg tacacaacat
    11161 cgcgtcgacc gtcagcagca cggccttgaa cagacggcgc gtgagcccga tcgcgcccat
    11221 cgccggtacc gagatgactc caacgccgag agctggactc gcctcggcga ctaccggatg
    11281 acgaccaacc ctcagcggac gaccggtgag acagacgacg aggtcccagc cttcctccag
    11341 catgcggcgg cgagccagct gaggaaggtc gacatctgcg cctgcgccgg cgaagggctc
    11401 cgtgccaacc acgactttcc acgacgtccc gtggaacctc cttgccaacg cagacggcag
    11461 tcgttccgcc agataagcgg cgagcttctc cggcaacccc gggcctgcga tcaggcccag
    11521 caccatctgc tgtgggcgct tctcctcaga gggcttggtg ggctcatcgg acatggcgag
    11581 tggaccttcg gggctcgtgc cacgacatct cgagccggat cggcgcgggg cccgcgagga
    11641 acgaccaccg gcggtagccc gggaaccgcg agaacgaccc gggctcgcat gctcggcgaa
    11701 gctcacgcgg acgtcgtcgt aagcgtcctc gagcacggcg cgtgtggaag cctccgacgc
    11761 gaccacctcg gggacgagcg gcaacgtcga gatgtcgttg atgaggagct gcgcgaggtt
    11821 cttgctcgcc gagatgccag gtgccctgac cgagtgccgg caccgactcg cctgacggaa
    11881 gcttgactgt acgaatgctc gccatgtgcc tcgttcgatc ggataccggc gcggcgccgc
    11941 ctgcaccgcc gactcgcctc cgaatgagct ggtagccgcg gtcgtgttcg gctccgcgtg
    12001 ctgaatgtag tgcctccacg cgaatcaata atcggagcaa cccgctcggc gatggcacga
    12061 cgccgcgggt gcgagaactc tgctcgcgcg gctccccatg gcgaactgct tcgcaacgct
    12121 tatcctgttt ccatggcgac ccaacaagca gtcatcatca cgctcgatat ggcgcagcaa
    12181 aagacgctcg gcgaatccat cgaagaggcg aacgcgcacg tcacgcggct tctgaccgaa
    12241 ggctggcgcc tcatgtgggc aactccgttc tcgggctcgc agaccaacac ggtggcgctg
    12301 atgctcgtga tcgaacgtgg ctagtgtccc gtatcaaaga acgatcgaca aagtcggacg
    12361 gtcgtgatct aagcgtggga tggcacgcac cgggcgaccg aagaccgaga ccctcacgct
    12421 gaccaccgag gaacgtgcgg cgctggaacg ccttgcagca agacctcgca gcggcggtca
    12481 catggccttc cgcgccaaga tcatcctgct gtgcgcggaa ggaacgaagt cgaacaagga
    12541 cgtcgcgcgc gagcttcgcg taggccgtgg ctccgtcggt aagtggcggt ctcgcttcat
    12601 caccgcggct agcgcgcgga cgagccgcaa tcgacctcca cggcagggct cgggtagcac
    12661 gcccaccagc tacgctgcgt caccagacga ggtcgaacag ctggagcaca cgcagccagt
    12721 gagcggtcac gataccgacg acatccgggt cgatgacctt gaagctgcgt gcgagcgcga
    12781 cggtcatgcc tccgatgatg taggggagct gggcgtacac ctcatcgtca taggagaggt
    12841 cgaactgggg cctcttaccc agcttctcga ggaccggggc caggccgagc tgctcctcgg
    12901 tatcgattgt gcggaagtcg tcgatgcacc tggcgagtcc cttcgtgatc ggtagatcgt
    12961 gcggaaggat gaccttgcgg ccgctgtcct gcgccacctc acacgcacgc cgcagaaggt
    13021 cagcgacctt gacatcgagg aacccttcgt agtggcgtag gtcctctttg tcgatatcca
    13081 cgccagcggc cacgcggaac aagcgctcga atcgagcgat gtgagtcggg cgaacgggtg
    13141 gcatcggttt tgggggctgg gtgactgtca tgagttggta ctccggtcgg gatcaccgcc
    13201 acgacgtgtc gacggtcgac ctcggtttga ggccttgttc ttgctcgatg gtcggatagt
    13261 ccgtgtagcc gtgctcgccg ccccccgtag aacgtcgtcg ggtccggctt gttgagcgac
    13321 gtggtgcggc ggaaccgatc gggagatcgg gagtggcaag gaacgcgcgg ccgaacgcga
    13381 tcagatccgc tttccccgcc tcgagccatt gctcggcggt ttcgcgatcg aacccgccgg
    13441 cgagcatcag cggaccgtgg aatttctcgc gggacgcaga acttgcggcg ggccggaccg
    13501 atcgatcgct tcggcgagca tcgtctccgg gatcgtgcga agccccttgc cggtgcgtgc
    13561 cttcactcgc ggtttctcgg gtctcttctc cggtatcaac atggtttcac cttgttctca
    13621 gctcacgtcg agtgggacct tgtacttcgg cggttcgaag acgtcgtcga ggtcgcgcag
    13681 gtccttcgac gtgagctgaa tgtcgagcgc gccgcggttt tcccgcacgt gttcggggtt
    13741 gcccgctctc gggatcgtaa tgacgtcggg ttggcggagc acccatgcga tcgcgatctg
    13801 cgcgggcgtc gcgtcgtgcc gcttggccac gcgccgtacc accggatggt cgaccaacgt
    13861 gcccccctcg acgggggagt acgccatgag ggggatgccc cggctgcgac accaaggcag
    13921 gaggtcccat tcgatgcctc gatgcgcgag gttgtagagc acctgatcgg tctgcggctc
    13981 gatctcgggg acctgaaaca ggtccgtcat gtcgtcgtgg tcgaagttgc tgacacccca
    14041 gtaccggatc ttcccggtgt cgatgagctc ttcgaatgcg tcgaccgtct cctcgagcgg
    14101 cacggtgcct cgccagtgga gcagatagag gtcgatgcgg tcggtggcga gccggcgcag
    14161 gctgtcctcg caggcctgga tggtcccgcg acgcgtcgcg tggctcggcc ggaccttgtc
    14221 gacgatgaag cactcgttgc gtctcccgac cattccgatg gcctcgcgga tcagatcttc
    14281 ggcgccgacg tacatctcgg cggtgtcgat gagcgtcatc ccgagatcga gcccgaggcg
    14341 cagtgcggcg atttcgtcct gccgcttgct cgggtgctcg ccgagatgcc aggtgccctg
    14401 accgagtgcg ggtaccgact cgcctgacgg aagcttgact gtacgaatgc tcgccatggt
    14461 gcctcgttac atcggatacc ggcgcggcgc ctcctgcacc gc
//
LOCUS       DASVOR010002521         2908 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_406919,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002521 DASVOR010000000
VERSION     DASVOR010002521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406919"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..630)
                     /locus_tag="VF516_46310"
     CDS             complement(<1..630)
                     /locus_tag="VF516_46310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115251.1"
                     /translation="MPRPRRPGGRVERDREDEANEWAMMFTMKDADVARLCDRMPMTL
                     DPVLQLARRCGVPPVAAAVRLMGVTVRFCAAVLSQDGVIQRASPSLRLLGFMDVSRFP
                     EGKPIGAGALARRFVDTGRLRRDPDLVPASAWIDGVSDDTWIRGAELAPAPAGAAEDA
                     GDTPGAPPRPRVDPWRSAARRTRLLDPRRGRVEAARRAPGRVLLGPRRGQ"
     gene            775..921
                     /locus_tag="VF516_46315"
     CDS             775..921
                     /locus_tag="VF516_46315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115252.1"
                     /translation="MLPKLVGEQAARQEVSDVNDVSGSTTVMPATATLGNLDAWEHLG
                     LRHR"
     gene            complement(918..1322)
                     /locus_tag="VF516_46320"
     CDS             complement(918..1322)
                     /locus_tag="VF516_46320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115253.1"
                     /translation="MTPRPTAGRMQMTCTVVVIPSAARTRVLLTEGPDELLRAVLPSP
                     SQLQYERAAITFLESLALWVNAKLHVVLSVDAKEAGCCLGLTDELGAGVRSLFFDVEV
                     RDRRARRRRGQRIRGIGDFADVRQLRLVPDGN"
     gene            1379..2272
                     /locus_tag="VF516_46325"
     CDS             1379..2272
                     /locus_tag="VF516_46325"
                     /inference="COORDINATES: protein motif:HMM:NF016629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HEX8115254.1"
                     /translation="MPAALRELIAWDTLHGESGSFVDPALADHHIDLLFSARLRTDAT
                     ELIHVLLEDQSTADPAMPLRTLSYRTRVWNRCRKERPDVWLPPILAVLVSHVPSGWTS
                     SRTLDDMFDPSVRAIPGLVASIPRSSLIIDDLAHLSNEDLQARSLGAFQKLALWLLRD
                     ARAPRRLLDSFDTWIVTFAEAERAPAGVDATTALLTYMFRVIDPGHHDQLRAKIGKLG
                     THVEEIAMTIAEQWREEGIAKGREEGRIAALRSQLSAKFGSQALDATCEARLQAATPA
                     AVDHYLQRVLVADSLAAVFED"
     gene            complement(2310..>2908)
                     /locus_tag="VF516_46330"
     CDS             complement(2310..>2908)
                     /locus_tag="VF516_46330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012828694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8115255.1"
                     /translation="CAGADLKERRGVSAAETGPYINAIAGAIDSWAASRKPTICALNG
                     SAYGGGLELALACDFRILVEGAELGLTEVRLGIMPGAGGTQRLPRLIGEARAKEMILL
                     GRRISAARALEIGLVNQVVPRAGLKAAVEALLAELAVCAPLSVRCAKAAIEGGYGKPM
                     EEGLGIERRYYDATLFSADRDEGLAAFAEGRPPRYEGR"
BASE COUNT          452 a         1070 c          977 g          409 t
ORIGIN      
        1 ttgcccgcgg cgcggtccca gaagcacgcg cccgggcgcg cgtcgcgcgg cctcgactcg
       61 accacggcga gggtcaagca gtcgagtacg acgtgcagca gagcgccaag ggtctacacg
      121 cggtcgcggt ggcgcacccg gagtatcgcc ggcgtcttcc gccgcgcccg ccggcgcagg
      181 cgccagctcc gcaccgcgga tccacgtgtc gtcgctcacg ccgtcgatcc acgcgctggc
      241 gggcacgagg tccggatcgc ggcggagtcg tccggtgtcc acgaaccgac gcgccagggc
      301 gcccgcgccg atcggcttgc cctcggggaa gcgcgacacg tccatgaacc cgagcaagcg
      361 cagcgacggg gaggcccgct ggatcacgcc atcctgggac agcacggcgg cgcagaaccg
      421 gaccgtcacg cccatcaggc gaaccgcggc ggcgaccggc gggaccccac aacggcgggc
      481 gagctggagc acggggtcga gcgtcatcgg catccgatcg cagagccgcg cgacgtcggc
      541 gtccttcatg gtgaacatca tcgcccactc gttggcttcg tcctcccggt cgcgctcgac
      601 ccggccaccg gggcgccgtg ggcgcggcac gcacagctca ttggccggga accggtcggc
      661 ctgtgcgatc ggacggcgtc gggcatcgac gagtcggtac cgcccccacc ggtccacgtg
      721 caaggtcacg gtgccactcg acgagcgcgt tgtcgttgta cgtcggatcg ccacttgcta
      781 ccgaagctcg ttggagaaca ggccgcgcgg caggaggtca gcgacgtcaa cgacgtctcg
      841 ggttcgacca ccgtgatgcc agcaacggcc acgctcggca acctcgacgc gtgggaacac
      901 ctggggcttc gccatcgcta gttaccgtcg ggaaccagcc gcagctggcg cacgtccgcg
      961 aagtcaccga tgccgcgaat gcgctgacct cgccgccgac gagcgcgccg gtcacggacc
     1021 tcgacatcga agaacagcga gcggacccca gcgcccaact cgtcggtgag gccgaggcag
     1081 cacccggcct ccttcgcgtc cacagacaac acaacgtgga gcttcgcgtt cacccacagc
     1141 gcgaggctct cgaggaacgt gatcgccgct cgctcgtact gcagttgcga cggtgacggc
     1201 aagaccgccc gcaacagctc gtcgggcccc tccgtgagca acacccgcgt ccgcgccgcc
     1261 gacgggatca caaccaccgt gcaggtcatc tgcatccgtc ctgccgtcgg ccgcggggtc
     1321 actcgtcaaa tcgtcgttcg aagctcccgc cgatgccgcg gcgctgctgc gcgagctgtt
     1381 gcctgctgcg ctccgagagc tcatcgcgtg ggacacgctg cacggcgagt ccgggtcgtt
     1441 cgtcgatccg gccctcgccg accatcacat tgatctgctg ttctccgcgc ggctgcgaac
     1501 tgatgccacc gagctgattc atgtcttgct ggaggaccag agcaccgcgg atccggcgat
     1561 gccgctgcgc acgctgtcgt atcgcacccg ggtctggaac cggtgtcgca aggagcgccc
     1621 cgacgtgtgg cttccaccga tcctcgctgt gctcgtcagc catgtgccga gcggctggac
     1681 aagctcgcgc acgctcgacg acatgttcga tccgagcgtg cgggcgatcc cgggcctggt
     1741 ggcgtcgatc ccgcgatcct cgctgatcat cgacgacctc gcccatctgt ccaacgagga
     1801 cctccaggcg cgctcgctgg gcgcattcca gaagcttgcg ctctggctgc tgcgcgacgc
     1861 tcgtgcgccg cggcgcctgc tggacagctt cgacacctgg atcgtgacct ttgccgaggc
     1921 ggaacgagcg ccggccggtg tcgacgcaac gaccgccctg ttgacataca tgtttcgagt
     1981 gatagacccg gggcaccacg accaactccg tgccaagatc ggcaagcttg gtacccacgt
     2041 cgaggaaatc gccatgacca tcgccgaaca gtggcgcgag gaaggcatcg cgaagggccg
     2101 cgaggaagga cggatcgccg cgttgcgctc ccagctcagc gcaaagttcg ggtcccaggc
     2161 gctggatgcg acgtgcgaag cccgcctgca ggcagcgacg cccgcggcgg tcgaccacta
     2221 tctccagcgc gtgctggtcg ctgattcgct cgccgcggtg ttcgaggact gaccaccgta
     2281 gggccacgta gccacgacga tcccggcccc tagcggccct cgtagcgcgg cggccggccc
     2341 tcggcgaacg ccgcgaggcc ctcgtcgcga tcggcgctga acagcgtggc gtcgtagtac
     2401 cggcgctcga tgccgagccc ctcctccatc ggcttgccgt agccgccctc gatcgcggcc
     2461 ttggcgcacc gcaccgagag cggcgcgcag accgcgagct cggcgagcag cgcctcgacc
     2521 gcggccttga gcccggcgcg cggcacgacc tggttgacca ggccgatctc gagcgcgcgc
     2581 gcggccgaga tccggcggcc gagcaggatc atctccttgg cccgggcctc gccgatcagc
     2641 cgcggcaggc gctgggtccc gccggcgccc ggcatgatcc cgagccggac ctcggtcagc
     2701 ccgagctcgg cgccctcgac gaggatccgg aagtcgcagg ccagcgcaag ctcgagcccg
     2761 ccgccgtagg ccgagccgtt cagcgcgcag atcgtcggct tgcgcgacgc cgcccagctg
     2821 tcgatcgcgc cggcgatcgc gttgatgtac ggcccggtct cggccgccga cacgccgcgg
     2881 cgctccttga gatcggcgcc ggcgcaga
//
LOCUS       DASVOR010002522         2887 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_262494,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002522 DASVOR010000000
VERSION     DASVOR010002522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262494"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="VF516_46335"
     CDS             complement(<1..552)
                     /locus_tag="VF516_46335"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8115256.1"
                     /translation="MAKGSAEVGSTANRYQILGKLAVGGMAEIFLARGANTTGLIRYC
                     VLKRILREKATDAQFVQMFLDEAKLAAHLQHPNIASVYDIGLLGDSYFFTMEYVHGET
                     VRSLLHRAKQRDRKVPLACALTIIAGAAAGLHHAHERNGNDGRPLGIVHRDVSPSNLM
                     ISYEGNLKIVDFGVAKAADRVETK"
     gene            805..2568
                     /locus_tag="VF516_46340"
     CDS             805..2568
                     /locus_tag="VF516_46340"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEX8115257.1"
                     /translation="MRSLEAALEREQKKVALVQEVSRALSQTENLDALLALIITKVTE
                     LMEAERSTLYLLTEDGRQVWSKVSQGSERIEIRLDLGEGIAGWVAKTREIVNIPDAYA
                     DQRFQPAVDHKTGYRTRSILSVPMLGTPGLVGVLQVLNKQDGPFTHADEELLMALASQ
                     AAVAIEHARLYHALREQNEELSRARRDLERRTRELNALYEVEKELSAALDLDDLLSRI
                     LAQAITVLGGGAGSIALLDADGSLRFRTVQGPAAPRLIERTLPHGTGLLGWSIKHRTP
                     LVVEDPGRDPRHAAEIAAEIGVAPKHLMVAPLIDGEDAIGGIEIVDQRRASRDGDGPW
                     SGDDLKLLVLIAAQAAGAISLARRRSEQSNRDRLASIGQMLAGLLHDLKTPMTIISGY
                     AQLMAAMDDPAQREKYVDQIQRQFDLMAGMTREVLAFARGDTDLVIRKVYVHRFAEEL
                     STQLGAAVQGRGIDFEVETRYDGIAYFDEQKLMRVFHNLASNAIEAMPDGGHLRVTID
                     KDGDQLVWAVRDTGPGIPDHVRGRLFELFATGRKGGTGLGLAIVKKIIDDHHGTIRPE
                     TGPSGTTFVIRMPLSRPADGE"
     gene            2573..>2887
                     /locus_tag="VF516_46345"
     CDS             2573..>2887
                     /locus_tag="VF516_46345"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEX8115258.1"
                     /translation="MLALRGIVRTFGRRRAVAGVSLEVAAGEAVLLVGRNGAGKTTLL
                     RIAAGFLDPDEGEVIVDGIAMARQRVRAQARLGYLPEHAPAPGELTVREHLYLRARQK
                     RVA"
BASE COUNT          437 a          947 c         1086 g          417 t
ORIGIN      
        1 cttggtctcg acgcggtcgg cggccttggc gacgccgaaa tcgacgatct tgaggttgcc
       61 ctcgtagctg atcatcaggt tggacggcga gacgtcgcgg tggacgatgc cgagcgggcg
      121 gccgtcgttg ccgttgcgct cgtgggcgtg gtggaggccg gcggcggcgc cggcgatgat
      181 cgtgagcgcg caggccagcg gcaccttgcg atcgcgctgc ttggcccggt ggagcaggga
      241 ccggacggtc tcgccgtgga cgtactccat ggtgaagaag tacgagtccc cgagcaagcc
      301 gatgtcgtag accgaggcga tgttggggtg ctggaggtga gcggcgagct tggcctcgtc
      361 gaggaacatc tggacgaact gggcgtcggt cgccttctcg cgcaggatcc gcttgagcac
      421 gcagtagcgg atcaggccgg tggtgttggc gccgcgcgcc aggaagatct ccgccatgcc
      481 gccgaccgcg agctttccca ggatctggta gcggttcgcg gtcgatccga cctcggcaga
      541 tcccttcgcc acggcgggat tgtcaggccg ccgcgtcgcg ggtgtcaaac caggtttccc
      601 gcggttctcg gcggtgtcat gcgatgtgag cgcgggcggc atggtggcgg cacggccgcg
      661 gcatgaaacg gccacgcgga cggcgaccgc cgccgacgat cggcagcgaa ccgcggctgt
      721 gctgggtgca cacgcaggcg ttgcggtacg atgtcgtcca tgtcggacgg tacgggactc
      781 gcgccaccaa gcgcacgaga tcccatgcgt tcgctggaag ccgccctcga gcgggagcag
      841 aagaaggtcg cgctggtcca ggaggtcagc cgtgcgctgt cgcagaccga gaacctcgac
      901 gcgttgctcg cgctgatcat caccaaggtc accgagctga tggaggcgga gcgctcgacg
      961 ctgtacctgc tgaccgagga cggccgccag gtatggtcga aggtgtctca gggcagcgag
     1021 cggatcgaga tccggctcga cctcggcgag ggcatcgccg gctgggtcgc caagacccgc
     1081 gagatcgtca acattccgga tgcctacgcc gatcagcggt tccagcccgc ggtcgaccac
     1141 aagaccggct accgcacgcg gtcgatcctg tcggtgccga tgctgggcac gccggggctg
     1201 gtcggcgtgc tgcaggtgct caacaagcag gacggcccgt tcacccacgc cgacgaggag
     1261 ctgctgatgg cgctggccag ccaggcggcc gtcgcgatcg agcacgcgcg gctgtaccac
     1321 gcgctgcgcg agcagaacga ggagctgtcg cgcgcgcgcc gcgacctgga gcgccggacc
     1381 cgcgagctca acgcgctgta cgaggtcgag aaggagctgt cggcggcgct cgatctcgac
     1441 gacctgctgt cgcgcatcct cgcccaggcg atcaccgtgc tcggcggcgg cgcggggtcg
     1501 atcgcgctgc tcgacgccga cggctcgctg cggttccgca ccgtgcaggg gccggcggcg
     1561 ccgcggctga tcgagcgcac gctgccgcac ggcaccgggc tgctcggctg gtcgatcaag
     1621 caccggacgc cgctggtggt cgaggatccg ggccgcgatc cgcgccacgc cgccgagatc
     1681 gcggccgaga tcggggtcgc gccgaagcac ctgatggtcg cgccgctgat cgacggcgag
     1741 gacgcgatcg gcgggatcga gatcgtcgac cagcgccgcg cgtcgcgcga cggcgacggg
     1801 ccgtggagcg gcgatgatct caagctgctg gtgctgatcg cggcgcaggc ggcgggcgcg
     1861 atcagcctgg cgcgccggcg cagcgagcag agcaaccgcg accggctggc gtcgatcggc
     1921 cagatgctgg ccggcctctt gcacgacctg aagacgccga tgacgatcat ctcgggttac
     1981 gcgcagctga tggccgcgat ggacgatccc gcacagcgcg agaaatacgt ggatcagatc
     2041 cagcgccagt tcgacctgat ggccggcatg acgcgcgagg tgctggcgtt cgcgcgcggc
     2101 gacaccgatc tggtgatccg caaggtctac gttcatcgtt ttgccgagga gctgtcgact
     2161 cagctcggcg cggcggtgca gggccgcggc atcgacttcg aggtcgagac gcgctacgac
     2221 gggatcgcgt acttcgacga gcagaagctg atgcgggtgt tccacaacct ggcgtccaac
     2281 gcgatcgagg cgatgcccga cggcggccac ctgcgggtca cgatcgacaa ggacggcgac
     2341 cagctggtgt gggcggtgcg cgacaccggg cccgggatcc ccgatcacgt gcgcggccgg
     2401 ctgttcgagc tgttcgcgac cggccgcaag ggcggcaccg gcctgggcct ggcgatcgtc
     2461 aagaagatca tcgacgacca tcacgggacg atccgtccgg agaccggacc gtccggcacc
     2521 acgttcgtga tccgcatgcc gctgtcgcgc cccgccgacg gcgagtgagc cgatgcttgc
     2581 gctgcgaggg atcgtccgca cgttcggccg ccgccgggcg gtcgccggtg tgtcgctgga
     2641 ggtcgccgcg ggcgaggccg tgctgctggt cggccgcaac ggcgccggca agaccacgct
     2701 gctccggatc gccgccgggt tcctcgaccc ggacgaaggc gaggtgatcg tcgacgggat
     2761 cgcgatggcg cggcagcgcg tgcgcgcgca ggcccggctc ggctacctgc cggagcacgc
     2821 gccggcgccg ggtgagctca cggtgcgcga gcacctgtac ctgcgcgccc gccagaagcg
     2881 ggtggcc
//
LOCUS       DASVOR010002523         7438 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_320556,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002523 DASVOR010000000
VERSION     DASVOR010002523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7438
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320556"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..1575)
                     /locus_tag="VF516_46350"
     CDS             complement(124..1575)
                     /locus_tag="VF516_46350"
                     /inference="COORDINATES: protein motif:HMM:NF006088.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEX8115259.1"
                     /translation="MSVAEPSVSEPPLFVDLDGTLVKTDLLLESLLILVKQLPWLIVL
                     LPLWLLRGRAYLKAEIARRADVAADTLVYDERVVGLVRAESERGRPVVLATASHAKYA
                     GAVAAHLGLFTGVLASDETRNLKGSRKLEAILAYTAGESFDYAGNAAADLPIWRKARK
                     VICVDAPGSVVRAVRAFAEPEELPRSRRSYLKLVVRSIRVHQWLKNSLVFVAIAAAHR
                     IADVEADLRVVAAFVVYSLCASSVYVLNDLTDLRADRLHPRKRNRPFASGALPLWLGL
                     VMSPVLLAISAAIAIAVMPPIFLVVMGTYYVASMAYNFAAKQLAVWDVVLLAGLYVLR
                     VLAGAAAVPVAPSVWLLAFSMFVFLSLALAKRYSEMHTLHKLGRGHAHGRGYLTEDMP
                     VLQSMGVASGYLAVLVMALYIHSPEVHALYPRPYVLWAICPLLLFWTTRIWLVTHRGE
                     MHDDPVVFALRDRVSLLIGALTVACLAIAAIQG"
     gene            2160..2288
                     /locus_tag="VF516_46355"
     CDS             2160..2288
                     /locus_tag="VF516_46355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115260.1"
                     /translation="MFRESYDWFCANRGKTAPSDAGGSPHRRAVREGVLRLVKRFS"
     gene            2702..3391
                     /locus_tag="VF516_46360"
     CDS             2702..3391
                     /locus_tag="VF516_46360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115261.1"
                     /translation="MRGSALVLVWAACAACHGGGASVDASEPRVYVVNTFAPHGPFFL
                     SSDVSFSDPAGHPIPGTRVPDPDTDAGQYGTVYAVMPDGGSLTMSIHESDSPTSNSDS
                     SWSIAGVKPGDHYRFDGSSVTLPAGCTRRTFTSFTIDATGTQASWDDPDAASVDARIV
                     HRTRGCQNSFPPITCVVTVEVDSSTATTVKVLVSGISTPTADLTSVDVVSCSSQTYDD
                     IRMRLTGQFAP"
     gene            3561..3956
                     /locus_tag="VF516_46365"
     CDS             3561..3956
                     /locus_tag="VF516_46365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115262.1"
                     /translation="MATDSFQVHFRAAAECMAVHRSIPSAEYLVSLTLDTDQADIVIS
                     YAREDERRVQLLCQALEAQGWSVFWDRRTNVTRDAISSFQKACDGGDMTGCTNLALAY
                     EHGTGVARDATNPRCRQGQLPLPEGLRRR"
     gene            4992..7031
                     /locus_tag="VF516_46370"
     CDS             4992..7031
                     /locus_tag="VF516_46370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115263.1"
                     /translation="MAAEARLAAASLVLDRLRGLIENEIPAGASSDDLKPLLVSLIGD
                     TRRDLEKTGGTGTYVGQIEMGTHFDQAQMSAQHSDLAGAEREYRAALVIGERELLKQP
                     GNAALRLDIAVTHRRLAALAKRRRDPAQAKAELALALAQTEALVHDEPSNAMARGNLC
                     EQYEARGEQLEADGSPDAKASYEQVVVCAKEVSPDLWVEIGEPLAEAHRRLASLHLQH
                     AEPKAAIDEYREAVKIAEPGADRFPAWFPPKKLVDDYIAIARLLEESGNVPDARRELE
                     KASQFIARFRAANPQSALALDQEWVIRSHILQDAARTGDSAGVAASLSAFLATEQLIE
                     NRIETETGELQQYVEEMLELAHRLVVAQAADAGVAVARKALGILNRHARESKSFAGPY
                     LLLTHEAAALMTVLGPDEPLAVPRAEIEACRKLTKLDRDHALRCGFAFLVRARAALAR
                     NDLAAVSRDSDAAMALWKQFGEDRTPEVLEFKVGSGALALRLLSAQGGFAAALRLSAQ
                     VLPLARERAGSASGAMDKDLANEALADVLLLTGIATLPTTSDQRLALLRESAALWEKD
                     RSALGDISRTACLVQLAKTLFDRQDLAGAKNAMNAARGVLAPHLAAGEPLTQDLVAVF
                     LMVEEETIEVAVAAKNRPICEEAAQQLQKLGELFGDGVARAAVRQLGARCAAIVP"
     gene            7053..7187
                     /locus_tag="VF516_46375"
     CDS             7053..7187
                     /locus_tag="VF516_46375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115264.1"
                     /translation="MTTVEVRPSGLPTPMVDLDPVDGLSCPSESYDDLRTLLTGRLAP
                     "
BASE COUNT         1215 a         2630 c         2511 g         1082 t
ORIGIN      
        1 ggcgcgggcg agatcgaggc ggtggaacat ccggtacgtg tagaacaccg acggcccggg
       61 tgtccacccg tgcgcccgcg cgagcgccgc tcgtcgggca ggagcgcgcc ggcgcccacc
      121 gcgtcagccc tggatcgccg cgatcgccag gcacgccacg gtcagcgcgc cgatcagcag
      181 gctgacgcgg tcgcgcagcg cgaacaccac cggatcgtcg tgcatctcgc cgcggtgggt
      241 cacgagccag atccgcgtgg tccagaacag cagcagcggg cagatcgccc acagcacgta
      301 gggccgcggg tacagcgcat ggacctcggg gctgtggatg tacagcgcca tcaccagcac
      361 cgcgaggtag ccgctcgcga cccccatcga ctgcagcacc ggcatgtcct cggtcaggta
      421 gccccggccg tgcgcgtggc cgcggccgag cttgtgcagc gtgtgcatct ccgagtatcg
      481 cttggcgagc gcgagcgaca ggaacacgaa catcgagaac gcgagcagcc acaccgacgg
      541 cgcgaccggc accgccgcgg cgccggccag cacgcgcagc acgtacagcc cggcgagcag
      601 caccacgtcc cacaccgcga gctgcttggc cgcgaagttg tacgccatcg acgccacgta
      661 gtacgtgccc atcacgacca ggaagatcgg cggcatcacc gcgatcgcga tcgccgcgct
      721 gatcgcgagc aacaccggcg acatcaccag cccgagccac agcggcagcg cccccgacgc
      781 gaacggccgg ttgcgcttgc gcgggtgcag ccggtccgcg cgcaggtcgg tcaggtcgtt
      841 gagcacgtac acgctcgacg cgcacaggct gtacacgacg aacgccgcga ccacccggag
      901 gtccgcctcg acgtcggcga tccggtgcgc ggcggcgatc gccacgaaca ccaggctgtt
      961 cttgagccac tggtggaccc ggatcgagcg caccaccagc ttcaggtacg agcgccgcga
     1021 ccgcggcagc tcctcgggct cggcgaacgc gcggaccgcg cgcaccaccg accccggcgc
     1081 gtcgacgcag atcaccttgc gcgccttgcg ccagatcggc aggtcggcgg ccgcgttgcc
     1141 ggcgtagtcg aagctctcgc cggcggtgta cgccaggatc gcctccagct tgcggctgcc
     1201 cttgaggttg cgggtctcgt cgctcgccag cacgccggtg aacagcccca ggtgagcggc
     1261 caccgcgccg gcgtacttgg cgtgcgacgc ggtcgcgagc accaccggcc ggccgcgctc
     1321 ggactccgcg cgcaccagcc ccaccacccg ctcgtcgtac accagcgtgt cggcggcgac
     1381 gtcggcgcgc cgcgcgatct ccgccttgag gtacgcacgg ccgcgcagca gccacagcgg
     1441 cagcaggacg atcagccacg gcagctgctt gaccaggatc agcaaggact ccagcagcag
     1501 gtcggtcttg accagcgtgc catcgaggtc gacgaacagc ggcggctcgg agaccgaggg
     1561 ttcggccacg ctcacggagt agccatagca cggccttgat cgtcgaggca tagctgctag
     1621 gatgccgcgc ttccacggcg gtccatctcg tcacgggagg ctccggcttc ctcggcaact
     1681 gatcgcgcgc cgcctgctcg cgcgcggcga gcgggtcaag atcctcgata tctgggagga
     1741 cccatcccgc ccgcgcgata tcgagtacat cgagtgtgac atccgcgacg tcgacggcgt
     1801 gcgccgcgcg ttccagggcg tcgacatcgt ccaccacaac gtcgcgctcg tcccgctcac
     1861 cgagtccggc gacaggttct gggacgtcaa cgtcaacggc agccggatcg ccgccgagga
     1921 ggccgcgcgc gccggcgcca aagcgttcat ccacatgagc tcgagcgcga tcttcggcgc
     1981 ccccgagcac tgcccggcgc cctccccgag cgcctctcga tcgccgccct ccagaccctc
     2041 gaccgggccg gcctcagccc gctcgcccct ggcactacct cacctacagc aagccgttct
     2101 tcttcgacgt ctcaccgctg ctcgccctgg gctggaaggc caggtactcc aacgacgaga
     2161 tgttccgcga gagctacgac tggttctgcg ccaaccgcgg caagaccgcg ccgagcgacg
     2221 ccggcggttc gccgcaccgc cgcgccgtcc gcgagggcgt cctgcgcctc gtgaaacgct
     2281 tctcgtagga gtgtgcaggg catagcgccc aggagaacgc cgacacgcga ctcgccgtgg
     2341 gctgctgcgc ggatgggtgt cggagccgcg cccgccgaat caccaccgac gcgtgcgcgg
     2401 gcgacgcgag gtatgtgcgg agcaagactc ggccaatcgt agtcacggtc gcggcgtccg
     2461 accccatcct gctcgcggtc gcccacggcg ggtcgcgttg acgtctcgtg atcgcgcccc
     2521 gaaggggtcc catcggaccc gcgcgcctcc cagcgcccgg gctgagctcg gcttcacagc
     2581 gtcctgatca gcggacgacc cgggggaccc gcgcatggct acacgctcct ggcgtgcgcg
     2641 gccgcccggc gtgccaggcc ctgccgcttc gcgccgcccc gcgcgtgcta gcttgccgcc
     2701 catgcggggc agcgcgctgg tcctggtctg ggcggcatgt gcggcgtgcc acggcggcgg
     2761 cgccagtgtc gacgccagcg agccgcgggt ctacgtcgtc aacaccttcg caccgcacgg
     2821 tcccttcttc ttgtcctccg acgtctcgtt ctcggacccg gccggccacc cgatccccgg
     2881 gacgcgggtg cccgatccgg acacggacgc cggccagtac ggcacggtct acgcggtcat
     2941 gcccgacggc ggcagcctga ccatgtcgat ccacgagagc gactccccga cgtccaattc
     3001 cgacagcagc tggtcgatcg ccggcgtgaa gccgggcgac cactaccgct tcgacggcag
     3061 ctcggtgacc ctgccggccg gctgcacgcg ccggacgttc acgagcttca cgatcgacgc
     3121 caccggcacc caggcctcgt gggacgaccc cgacgccgcc tccgtcgacg cccggatcgt
     3181 gcatcgcacc cgcggctgcc agaactcgtt tccgccgatc acgtgcgtcg tgaccgtcga
     3241 ggtcgacagc agcaccgcga cgacggtgaa ggtcctggtc tccggcatct cgacgccgac
     3301 cgccgatctg acctccgtcg acgtcgtctc gtgctcgagc cagacctacg acgacatccg
     3361 catgcggctc accgggcagt tcgcgccgta gtggccccgc cgagaccacc ccgcagcacg
     3421 atgcgccatg acatggcgcg ccaaaccggc gtcatctcgc cgcgtgggcg caataatcgc
     3481 gtacttcgcc cggtatgcgg ccagcacacg aacgcattcc cacgtaactt ccgtccgctc
     3541 ggtccagtcc gccagacggc gtggcaaccg acagcttcca ggtgcatttt cgtgcagccg
     3601 ctgagtgcat ggcggtacac cgctcgatac cgtctgctga atatctcgtg tcacttaccc
     3661 tggatacaga ccaagcggat attgtcataa gctatgcgcg tgaagatgag aggcgtgtgc
     3721 agctcctctg tcaggcgctc gaagcgcaag ggtggtctgt gttctgggat cgccgtacca
     3781 acgtcacccg cgatgccatc tcttcctttc agaaagcctg cgacggcggc gacatgacag
     3841 gctgcaccaa cctcgccctt gcctatgaac atggcaccgg cgtcgcccgc gatgccacca
     3901 acccgcgatg ccgccaaggc caactccctc taccagaagg cctgcgacgg cggtgacaag
     3961 catgcctgct cacgtatcaa gtaacttgtc gaagggagtc gttgcgcggg tccaacccct
     4021 tgaacgcctt gctcgcgacc ttcgtgcttc tgctccacga tcatggaccg cgctggcagg
     4081 atcagctgtg acagtactcg aagcagactg cgatctcggg cggtagtcct ccgagcgact
     4141 gctacccgtg atgatcggcg ggcccggcga tcgggcctcc atggtgctcg acgccgtgac
     4201 ctccggccgg gccaggcggg atgatcaatc atctcgcgct tctgcagtga cgcgtgggct
     4261 tgcggcgcgc accctccccc ggccgatccc gacgtgggac ctcgatcggc cccgctcggc
     4321 ggcactggat cggcgaagat gatgagatgg ggatcgcagg gaccgagccg gaatacaagt
     4381 atgttgcgtt catcagctac gcgcgcgccg ccgatgggct cgttgcttcg gaggtgttcg
     4441 aggcgctcga gacctatcgg atcccgaagc ggatccgcga ggaagcccga gtcggcaagc
     4501 ggtgcggccg gctgttcaag gacgccgacg aactggcggc cgagagcaat ctgcccgtgg
     4561 cgatcacgga cgcgctcgcc agctcgaagt acctgatcgt cgtgtgctca cccgaggcgc
     4621 gggcgcggcc gtggatcaac tgggagatcg agcatttcat tcagctcggg cgaaaagacc
     4681 atgtcctcgc ggtcctggtc cggggcgagg ccaccgacgc atttcctcct gcactcgtac
     4741 cgctcgatcc gatcgcgatc gatctgcgcg cgacgacccg gcgagcgcgg cgccggcagc
     4801 tcggggtcga gaagctccgg ctcctcgcac gtctgatcgg ctgcgagttc gatgcactcc
     4861 gccagcgcga tgcgcagcga gcggtcgccc gatcgcgcac gatcgccggt gttgccgttg
     4921 cggtcgccgc ggttgcaatc gcggtgctga tctacgtcct cgatctccga cgtgtgtccg
     4981 aggaacggcg ggtggctgcc gaggcgcggc tcgccgccgc gagcctcgtg ctcgatcgac
     5041 tccgtggcct gatcgagaac gagatccccg cgggggccag ctccgatgat ttgaagccgc
     5101 tgctggtcag cctgatcggc gacacgcgac gcgacctgga gaagaccgga gggactggaa
     5161 cgtacgtcgg ccagatcgag atgggtacgc atttcgatca ggcgcagatg tcggcccagc
     5221 attccgatct ggccggtgcg gagcgcgagt accgagcggc gcttgtgatt ggcgaacgcg
     5281 agctcttgaa gcagcctggc aacgccgcgc tccgcctgga tatcgccgtg acccaccgac
     5341 gcctcgcggc cctggccaag cgtcgccgcg atcccgccca ggcgaaggcg gagctcgcac
     5401 tcgcgctcgc ccagaccgag gcgctggtcc acgatgagcc cagcaatgcg atggcacgag
     5461 ggaacctgtg cgagcagtac gaggcgcgag gtgagcaact cgaggccgac gggagtcccg
     5521 acgccaaggc gagctacgag caggtcgtgg tgtgcgcgaa ggaggtatcg ccggatctgt
     5581 gggtcgagat cggcgagccg ctcgccgaag cacatcgccg actcgcgtcg ttgcacctgc
     5641 aacacgcgga gccgaaggca gcgatcgacg agtacaggga ggccgtgaag atcgcggagc
     5701 caggtgctga ccggtttcca gcctggttcc cgcccaagaa gctggtcgac gactacatag
     5761 ccatcgcgcg gctgctcgag gagagcggca acgtgccaga tgcccggcgg gagctcgaga
     5821 aagcgtcgca gttcatcgcg cgattccgcg ccgcgaaccc acagagtgcg ctggcgcttg
     5881 accaggaatg ggtcatccgg tcgcacatcc tgcaggatgc cgcgcgcacc ggcgactccg
     5941 caggggtcgc cgcgagcctg tcggcgttcc ttgcgaccga gcaactgatc gagaaccgga
     6001 tcgagaccga gaccggcgag ctgcaacagt acgtcgagga gatgctcgag ctcgcccacc
     6061 gcctggtggt cgcgcaagcg gccgacgcag gcgtcgcggt ggcccgcaag gcgctcggca
     6121 tcctgaaccg acatgccagg gagtcgaagt cgttcgccgg cccctacctg ctgctcacgc
     6181 acgaggccgc cgcgttgatg acggtgctcg ggcccgacga gccgctggcg gtaccacgcg
     6241 cggagatcga agcgtgccgc aagctgacca agctcgatcg ggaccacgcg ctgcgctgcg
     6301 ggttcgcatt tctcgtgcgc gcgcgagcag cgctcgcacg gaacgatctc gcggcagtgt
     6361 cccgcgacag cgacgcggcg atggcgctgt ggaagcagtt cggcgaggac cgcacacccg
     6421 aggtcctcga attcaaggtc ggatctggcg ctctcgcgct ccggttgctc tcggcgcagg
     6481 gcgggttcgc ggccgcgctc cggctgagtg cgcaggtctt gccgctcgcg cgggagcgcg
     6541 caggctcggc gtccggagcc atggacaagg acctcgcgaa cgaggcgctt gcagatgtcc
     6601 tgctgctcac tggcatcgcg accctgccga ccacgtccga tcagcgactt gccctgctgc
     6661 gtgaatccgc ggcgctctgg gagaaggatc gcagcgcgct cggcgacatc agtcggacgg
     6721 cgtgcctcgt gcaactggcg aagacgctgt tcgatcgcca ggatctcgcg ggcgccaaga
     6781 acgcgatgaa tgctgcgcgg ggggtgctcg cgccgcatct cgccgcgggt gagcccctga
     6841 cacaggatct ggtcgcggtg ttcttgatgg tcgaggagga gacgatcgag gttgccgtcg
     6901 ccgcgaagaa caggcccatc tgcgaggaag ccgcgcagca gctacagaaa ctcggcgagc
     6961 tgttcggcga tggcgtggcg cgtgcggcgg tcaggcagct cggcgcccgt tgcgccgcga
     7021 ttgttccata gaccgagcgt ggcgaggatc cggtgacgac ggtggaggtc cggccctccg
     7081 gcctcccgac accgatggtc gatctggacc cggtcgacgg tctctcgtgc ccgagcgaga
     7141 gctacgacga cctccgcacg ctgctcaccg gacggctcgc gccgtagagc acccgccatg
     7201 gcgccgcgtg agccgccgca cccgctgccg cgatcgggac ggtacagctt gccaggtcgc
     7261 gcggggaagc ttcctgccgc ggccggcgta gcatatggaa gacaggaggc ggcgtgagct
     7321 tcgaacgcag ggtgcagcga atgacgcagg tgggcgggcc ggcggcgggc gaggccatcg
     7381 ccgccggcaa gcgcacgctc accgagcagc tcgtgcagcg ccgcgccgag ggcgcacc
//
LOCUS       DASVOR010002524         1946 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_203425,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002524 DASVOR010000000
VERSION     DASVOR010002524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1946
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203425"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VF516_46380"
     CDS             <1..299
                     /locus_tag="VF516_46380"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEX8115265.1"
                     /translation="VGIVLDERAVPVRDEVRAAGELLGIDPLLVANEGKAVIGVRAAA
                     AGRVLDALRSHPLGRQAAVVGECVAERPGSVIADTGFGRRLVGEAEGELLPRIC"
     gene            299..1132
                     /locus_tag="VF516_46385"
     CDS             299..1132
                     /locus_tag="VF516_46385"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEX8115266.1"
                     /translation="MGDHIRIDRHGAVGVLTIARRPRFNSLDVATAQDLRKAGLQLAR
                     DRAIRAVVLRGDGGVFCSGADLKYIAVGGDGDVAYLSPDARPPSGGYGAIFKQILEYI
                     HSTISEIRRAPKPWIAAVDGPAAAGGLGLAIACDLVVASERSTFEWAYGKTGLTGAES
                     TTFFLPRLVGLRKAMELVLLSPRLTAREALAIGLINEVTANIDLEARALALAAHLAEA
                     PTTAVGVAKQLIGRAAGMDRLDGHLDEELRELARIADGPNFTEGLAAFLARRAPAFGV
                     E"
     gene            1132..1473
                     /locus_tag="VF516_46390"
     CDS             1132..1473
                     /locus_tag="VF516_46390"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HEX8115267.1"
                     /translation="MHEYSIIASLVDRVQREALARPGAIVRRLHVRIGELAGVEIPLL
                     ATAFETFRARTVSEDAELAIARVPAVWRCARCDRELPAGAVLRCCDRPARLAGGDDIF
                     LDRIEMEVRDV"
     gene            1466..>1946
                     /gene="hypB"
                     /locus_tag="VF516_46395"
     CDS             1466..>1946
                     /gene="hypB"
                     /locus_tag="VF516_46395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HEX8115268.1"
                     /translation="MCEDCGCGDPALVPVDVHEHILAGNDALAAHLREHFLEAGVLAI
                     NLMGSPGSGKTAVLEQTARAYRARGDARRLGAVSGDLATDRDARRLEAAGIDARAITT
                     GSACHLDARLVHDALHHMPWRRFDTFFIENVGNLVCPAIYDLGQAANVVALAVTEGED
                     "
BASE COUNT          242 a          728 c          709 g          267 t
ORIGIN      
        1 gcgtcgggat cgtgctcgac gagcgcgccg tgccggtccg cgacgaggtg cgcgccgccg
       61 gcgagctgct cggcatcgat ccgctgctgg tcgccaacga gggcaaggcg gtgatcggcg
      121 tgcgcgccgc ggcggcaggc cgcgtgctcg acgcgctgcg ctcgcatccg ctcggccgcc
      181 aggccgccgt ggtcggtgag tgcgtcgccg agcggccggg cagcgtgatc gccgataccg
      241 ggttcggccg ccgcctggtc ggcgaggccg agggtgagct cttgccgagg atctgctgat
      301 gggagaccac attcgcatcg atcgccatgg cgcggtcggc gtgctgacga tcgcgcgccg
      361 cccgcggttc aactcgctcg acgtcgcgac cgcccaggat ctgcgcaagg ccggcctcca
      421 gctcgcccgc gaccgcgcga tccgcgccgt ggtgctgcgc ggcgacggcg gcgtgttctg
      481 cagcggcgcc gacctcaagt acatcgcggt cggcggcgac ggcgacgtcg cgtacctgtc
      541 gcccgacgcg cggccgccga gcggcggcta cggcgcgatc ttcaagcaga tcctcgagta
      601 catccactcg acgatctccg agatccggcg cgcgcccaag ccgtggatcg ccgcggtcga
      661 cggcccggcc gccgccgggg gcctcggcct ggcgatcgcg tgcgacctcg tggtcgcgtc
      721 cgagcgctcg acgttcgagt gggcgtacgg caagaccggg ctgaccggcg ccgagagcac
      781 gacgttcttc ctgccccgcc tggtcgggct gcgcaaggcg atggagctcg tgctgctcag
      841 cccgcggctg accgcgcgcg aggcgctcgc gatcggcctg atcaacgagg tcaccgccaa
      901 catcgacctc gaggcccgcg cgctcgcgct cgccgcccac ctcgccgagg cgccgaccac
      961 cgcggtcggg gtcgccaagc agctgatcgg ccgcgccgcc gggatggacc ggctcgacgg
     1021 ccacctcgac gaggagctgc gcgagctggc gcggatcgcc gacggcccga acttcaccga
     1081 gggcctcgcc gcgttcctgg cgcgccgcgc gccggcgttc ggggtcgagt gatgcacgag
     1141 tactcgatca tcgcgtcgct ggtcgaccgc gtgcagcgcg aggccctggc ccggccgggc
     1201 gccatcgtgc gccggctcca cgtccggatc ggcgagctcg ccggcgtcga gatcccgttg
     1261 ctcgccaccg cgttcgagac gttccgtgcg cgcacggtga gcgaggatgc cgagctcgcg
     1321 atcgcccggg tgccggcggt gtggcggtgc gcgcgctgcg accgcgagct gccggccggc
     1381 gcggtgctgc ggtgctgcga ccggccggcg cggctggccg gcggcgacga catcttcctc
     1441 gatcggatcg agatggaggt tcgcgatgtg tgaagactgc ggatgcggtg acccggcgct
     1501 cgtgccggtc gacgtccacg agcacatcct cgccggcaac gacgcgctcg ccgcccacct
     1561 gcgcgagcac ttcctcgagg ccggcgtgct cgcgatcaac ctcatgggct cgccgggctc
     1621 cggcaagacc gcggtcctcg agcaaaccgc gcgtgcctac cgcgcccgcg gtgacgctcg
     1681 ccgcctgggc gccgtatccg gcgacctggc gaccgaccgg gacgcccgtc gcctcgaggc
     1741 cgccggcatc gacgcgcgcg cgatcacgac cggctcggca tgccatctcg acgcccgcct
     1801 ggtgcacgac gcactccatc acatgccatg gcgccgcttc gataccttct tcatcgagaa
     1861 cgtcggcaac ctggtgtgcc cggcgatcta cgacctcggc caggccgcca acgtcgtggc
     1921 gctcgcggtc accgaaggcg aggaca
//
LOCUS       DASVOR010002525         1627 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_406975,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002525 DASVOR010000000
VERSION     DASVOR010002525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_406975"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="VF516_46400"
     CDS             complement(<1..349)
                     /locus_tag="VF516_46400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115269.1"
                     /translation="MIVPRILTLALSCAAASAGASPRSEPTAGRAVFTGATTPHATSI
                     GVDPAALGLGSVDQLYIAFTSVLDQLHVALDRGPDNQAPGGRARDVELGPGAMLAFIY
                     HLAGDSLTLGFEAH"
     gene            384..>1627
                     /locus_tag="VF516_46405"
     CDS             384..>1627
                     /locus_tag="VF516_46405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115270.1"
                     /translation="MFARVLPIAVATFTFGTAGALALGAAGCEKLDHENIDKWAHTSK
                     GPAKLIDAVTRDGADPDLAAHAAANLIRRDDDREVYAAFETMPAARRTQVIARLAPRM
                     WDIARVESERELPGKSQTGAKDALVRIRRWADEPTRQQIDGYLIDWYCVASYEDRARA
                     GFSTGAPVMRMVGPAAGRKLIAVVNGVIAAPGQAGVRNRIGDQLLLGLAATGTPDAVK
                     YVLDLARMDRGDPTLASRALSALYKAYVAPDGFEPADRGALMPSLPPLVSIARDDRMS
                     GRPANDAVALIQAVGPPHCLAPLIGMLGAPHPDPRFKFVVVQGALRCGGPAAVVDVVR
                     AMPAGGYAQDEIDGAISGEIARMTPRDRAQAAARALLGEPSTLAVWVGIEALGAMKAS
                     DDAPRVAALASRREPLIGYWGQR"
BASE COUNT          223 a          612 c          589 g          203 t
ORIGIN      
        1 tgtgggcctc gaagcccagc gtcaggctgt cgccggcgag gtggtagatg aacgcgagca
       61 tcgcgcccgg gccgagctcg acgtcccggg cccgcccacc gggcgcctgg ttgtccggcc
      121 cgcgatcgag cgccacgtgc agctggtcga gcacgctggt gaacgcgatg tacagctggt
      181 cgaccgagcc gagcccgagc gcggcgggat cgaccccgat cgacgtcgcg tgaggcgtcg
      241 tggcgccggt gaacaccgca cggccggcgg tcggctcgct gcgcggcgac gcccccgccg
      301 acgcagccgc gcacgacagc gccagcgtga ggatgcgtgg aacgatcatc gcgcggaacg
      361 tatcaccacg ttaagctccc gccatgttcg cgcgcgtgtt gccgatcgcc gtggcgacct
      421 tcactttcgg caccgccggt gcgctggcgc tcggcgccgc gggctgcgag aagctcgatc
      481 acgagaacat cgacaagtgg gcgcacacca gcaagggccc ggcgaagctg atcgacgcgg
      541 tgacccgcga cggcgccgac cccgacctgg cggcccacgc cgccgccaat ctgatcaggc
      601 gcgacgacga tcgcgaggtc tacgccgcgt tcgagaccat gccggccgcc cgccgcaccc
      661 aggtgatcgc ccgcctcgcg ccgcggatgt gggacatcgc gcgggtcgag agcgagcgcg
      721 agctgccggg caaatcccag accggcgcca aggacgcgct ggtccgcatc cggcgctggg
      781 ccgacgagcc gacccggcag cagatcgacg gctacctcat cgactggtac tgcgtcgcct
      841 cctacgagga tcgcgcacgc gccgggttct ccaccggcgc gccggtgatg cggatggtcg
      901 ggccggcggc cgggcgcaag ctgatcgccg tcgtcaacgg cgtgatcgcg gcacccggcc
      961 aggccggggt ccgcaaccgg atcggcgacc agctgctgct cggcctcgcc gcgaccggca
     1021 cccccgacgc ggtcaagtac gtgctcgatc tcgcgcggat ggatcgcggc gatccgacgc
     1081 tggcgagccg cgcgctgtcg gcgctgtaca aggcctacgt cgcgcccgac ggcttcgagc
     1141 ccgccgaccg cggcgccctg atgcccagcc tgccgccgct cgtcagcatc gcccgcgacg
     1201 accggatgtc gggccggccc gccaacgacg cggtcgccct gatccaggcg gtcggtccgc
     1261 cgcactgcct ggccccgctg atcgggatgc tcggcgcacc tcaccccgac ccccggttca
     1321 agttcgtcgt cgtccagggc gccctgcgct gcggcgggcc ggccgccgtc gtcgacgtgg
     1381 tccgggcgat gccggccggc ggatatgccc aggacgagat cgacggcgcg atctcgggcg
     1441 agatcgcccg gatgaccccg cgcgaccggg cccaggccgc cgcccgggcg ctgctcgggg
     1501 agccctcgac gctggccgtc tgggtgggga tcgaggcgct gggtgcgatg aaagcatccg
     1561 acgacgcacc tcgggtcgcc gcgctggcct cccgccgcga gccgctcatc gggtactggg
     1621 gtcagcg
//
LOCUS       DASVOR010002526         4728 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_436578,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002526 DASVOR010000000
VERSION     DASVOR010002526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436578"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2390)
                     /locus_tag="VF516_46410"
     CDS             complement(<1..2390)
                     /locus_tag="VF516_46410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115271.1"
                     /translation="MGILGDFGKTIASSAPTLERLIAAAELHHAFSFDPISHGVTRLD
                     YEPRCSASCAEPGCQAIWVGSKAGEIFHLRFGADEPRSTKSWVNRSVRALVHVHRIGM
                     LIAGTDHGHLVLFEGDGEQATQRYVHLESWFEQRGTQPCPYDPGHRIDSDEDPFNDGI
                     TAILELPAEDPAVVDIVVATRYPALYVVRLQRDALAVRARHLLPGWTQCLTTLGPSGD
                     ENLVCASRGGELLEWRCRALRDDPTAAPVPTLTHLLPNAIGKRSGQLPEQQTTLLIGA
                     TDGLYMCRTGAATPEAARPVQLAATRSAVLSIAVVTIRAPRASEPMTYVALGLEDGRL
                     RVIEERALFGVLDGRDALQAHDFPVVLGDAVLALEVLASDDPTACFVFAALRDHRVRL
                     YRACSREALCEQLDALWRTAIDEPAAIRPAALGALLVREAELRRSVGSKHENALRYWL
                     ADEVLRRWTASGAERVVARACEIAKGADEKVLYRLSARIGDIAGTDVNALIRLSMACL
                     VAMPERDARRWRAFVSYHLKQFHACVRRVADHADLSRLQYWGRFVRKYLLLGETFADK
                     RYRIESLMKRNRETRKYLDALIYAAQLERQRYDLKWSEIACQDDDGRDQEIARVDLID
                     DCAIVVTASAEVVFFDVQGERLPIFSSQGCLDRLVPLRGDRASGLIRTRAARVVRRDG
                     TWVRIALSWAGASTLASHQLRVTIFDLRLTRSGVMVEGVHVATTSQSRRGGAAELEAH
                     GLVGLPNQDAFLVGLDSSESPLALLKLAEDGPDGPTWVLSLLEMSRAPGSPDALPAV"
     gene            complement(2495..3229)
                     /locus_tag="VF516_46415"
     CDS             complement(2495..3229)
                     /locus_tag="VF516_46415"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEX8115272.1"
                     /translation="MIALVNVMTAGCQTQLDQIDGAFYNGDHRLVHCGVNLDTSAKVS
                     IDAIDSALDRARDRGEVVELYTHHPGITVPISKIEHVLTGAHDRGLAFFTYSDFVHER
                     PVVGGVALSFDDTSVDAWFALRPLFDQYGAKVTFFVSRYPGIGPAQRAELQQLSAEGH
                     AVEPHTVNHLRAPDYVEEHGLNAYLHDEVDPSFEMLRADGFVVDAFAYPFGARTDQID
                     HGVGKRVSVLRSVAFTYEIVGDPCPN"
     gene            3257..4054
                     /locus_tag="VF516_46420"
     CDS             3257..4054
                     /locus_tag="VF516_46420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115273.1"
                     /translation="MDVSSAVAALGVASMLWTAQDAQAQPDVPPDDIVTRPLVLEPGA
                     VDVRLTLAINIQQRAITEPTALSPDAWWGVLPRLTIGLIHSDASLDQIATSGSFCVKT
                     STSTCAHLYKGSGIDVRYSALEGQFAIAPRLRAVIRDTDPFKPAVTLGARLRWAHGRF
                     AITGDPYLRVPLANASLGNYTEINLPVWLAVQPRAGWMIALRGGFESDLNVVRDGGHI
                     PFALDVAARATDHVDLGLEGGWGSLLGPLHDARHATIMLAAGWHGVP"
     gene            complement(4075..>4728)
                     /locus_tag="VF516_46425"
     CDS             complement(4075..>4728)
                     /locus_tag="VF516_46425"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEX8115274.1"
                     /translation="DMPIGILGSLAICTVLYILFAHVLTGIATVEDFRTAGREASVAY
                     AITKHMPGYAWLAKLVTVAILAGFSSVILVMLMGQSRVFFSMSRDGLVPKVFSDVHPK
                     YRTPWKSNMLFFVFVGVFAAFLPEDIVGEMTSIGTLFAFVLVCIGVWIMRVRDPHAPR
                     QFKTPLVPVVPILGIAANMLMIFGLGWTNWLRLGVWLAIGLVIYFAYGQYHSKLRSA"
BASE COUNT          747 a         1668 c         1650 g          663 t
ORIGIN      
        1 acggcgggca gggcgtccgg cgagcccggc gcgcgcgaca tctccagcag cgacagcacc
       61 caggtgggcc catcggggcc gtcctcggcg agcttgagca gcgcgagcgg cgactcgctc
      121 gagtcgagcc cgaccaggaa cgcgtcctgg ttggggaggc cgaccagacc atgggcctcc
      181 agctcggcgg cgccgccgcg gcgagactgg ctggtggtgg cgacgtggac accctcgacc
      241 atgacgccgc tccgggtgag ccgcaggtcg aagatggtga cccgcagctg gtgagacgcc
      301 agcgtgctcg ccccggccca gctgagcgcg atccggaccc aggtgccgtc ccggcggacg
      361 acgcgcgccg cgcgggtgcg gatcagcccc gacgcacgat cgccgcgcag cggcaccagt
      421 cgatcgaggc aaccctggct cgagaagatc ggcagccgct cgccctggac gtcgaagaac
      481 acgacctccg ccgaggccgt gaccacgatc gcgcagtcgt cgatcaggtc gacgcgcgcg
      541 atctcctggt cgcggccgtc gtcatcctgg caggcgatct ccgaccactt gagatcgtag
      601 cgctggcgct cgagctgcgc cgcgtagatc agcgcgtcga ggtacttgcg ggtctcgcgg
      661 ttgcgcttca tcaggctctc gatgcggtag cgcttgtcgg cgaacgtctc tccgagcagg
      721 aggtacttgc ggacgaaccg cccccagtac tgcagccgcg acagatcggc gtgatccgcg
      781 acgcggcgca cgcaggcgtg aaactgcttg aggtggtacg atacgaaggc gcgccagcgc
      841 cgcgcgtcgc gctccggcat cgcgaccagg cacgccatgc tgagccggat cagcgcgttg
      901 acgtcggtgc cggcgatgtc tccgatccgc gcggacagcc ggtacagcac cttctcgtcg
      961 gcgcccttgg cgatctcgca ggcgcgggcg acgacgcgct ccgcaccgga cgccgtccag
     1021 cgccgcagga cctcgtcggc gagccagtag cgcagcgcgt tctcgtgctt ggacccgacc
     1081 gagcggcgca gctcggcctc ccgcacgagg agcgcgccga gcgcggcagg tcggatcgcg
     1141 gccggctcgt cgatcgccgt ccgccacagc gcgtcgagct gttcgcacag cgcctcgcgg
     1201 ctgcacgccc ggtacagccg cacccggtgg tcgcgcaacg cggcgaacac gaagcacgcg
     1261 gtcgggtcgt cggacgcgag cacctccagg gcgagcaccg cgtcgccgag cacgaccggg
     1321 aagtcgtgcg cctgcagcgc gtcgcggccg tcgagcacgc cgaacagcgc ccgctcctcg
     1381 atcacgcgga gccggccgtc ctccagcccg agcgccacgt aggtcatcgg ctcgctcgcg
     1441 cgcggggccc ggatcgtcac gaccgcgatc gacagcaccg cgctgcgcgt cgcggcgagc
     1501 tggaccggcc gcgcggcctc cggcgtcgcc gccccggtcc ggcacatgta caggccgtcg
     1561 gtcgcgccga tcaggagcgt ggtctgctgc tccggtaact gccccgaccg cttgccgatc
     1621 gcgttgggca agaggtgggt cagcgtcggc accggagcag ccgtcgggtc gtcgcgcagc
     1681 gcgcggcacc gccactcgag caactcgccg ccccggctcg cgcagaccag gttctcgtcg
     1741 ccggacgggc cgagcgtggt caggcactgc gtccatcccg gcagcaggtg ccgggcacgg
     1801 accgccaggg cgtcgcgctg cagccggacc acgtacagcg ccgggtagcg ggtcgccacg
     1861 acgatatcga cgaccgccgg gtcctcggcc ggcagctcca ggatcgccgt gatcccgtcg
     1921 ttgaagggat cctcgtcgct gtcgatccgg tggcccgggt cgtaggggca cggctgcgtg
     1981 ccgcgttgct cgaaccacga ctccaggtgg acgtagcgct gcgtggcctg ctcgccatcg
     2041 ccctcgaaca gcacgaggtg tccgtggtcg gttccggcga tcagcatgcc gatgcgatgc
     2101 acgtggacga gcgcccggac gctgcgattg acccaggact tggtggatct cggctcgtcg
     2161 gccccgaacc gcaggtggaa gatctcgccc gccttcgagc cgacccagat cgcctgacag
     2221 cccggctcgg cgcacgacgc gctgcagcgc ggctcgtagt cgaggcgcgt cacgccgtgg
     2281 ctgatcggat cgaacgagaa cgcgtggtga agctcggccg ccgcgatcag ccgctccagc
     2341 gtgggcgccg agctggcgat cgtcttgcca aagtcgccca gtatccccat gttgcgcctc
     2401 ctgcgagcat cgtcacggca ccgcgtggcc acgcagcacc cggggatcag gcgatcatcc
     2461 ccggcccgtc acgacatcga aaatgcgaag gcgatcaatt cggacaggga tcgccgacga
     2521 tctcgtaggt gaacgcgacc gagcgcagca ccgagacccg cttgccgacc ccgtggtcga
     2581 tctggtcggt ccgcgcgccg aacgggtagg cgaacgcgtc gaccacgaag ccgtcggcgc
     2641 gcagcatctc gaacgacgga tcgacctcgt cgtgcaggta ggcgttgagg ccgtgctcct
     2701 cgacgtagtc cggcgcgcgc aggtggttca ccgtgtgggg ctcgaccgcg tgaccctcgg
     2761 cggacagctg ctggagctcg gcgcgctggg ccgggccgat gcccgggtag cggctgacga
     2821 agaacgtgac cttggcgccg tactggtcga acagcgggcg cagcgcgaac cacgcatcga
     2881 ccgaggtgtc gtcgaacgac agcgccacgc cgcccaccac cgggcgctcg tgcacgaagt
     2941 cggagtacgt gaagaacgcc aggccgcgat cgtgcgcgcc ggtcagcacg tgctcgatct
     3001 tgctgatcgg gacggtgatg ccggggtggt gggtgtagag ctcgacgacc tcgccgcggt
     3061 cgcgcgcccg atcgagcgcc gaatcgatgg catcgatgct gaccttcgcg ctggtatcga
     3121 ggttcacccc gcagtgcacc aggcggtgat cgccgttgta gaacgcgccg tcgatctggt
     3181 cgagctgggt ctggcagccc gcagtcatga cgttcacgag cgcgatcacc gcgatgcggc
     3241 gtgctagggt cgccgcgtgg acgtctcgtc ggcggtcgcg gcactcgggg tcgcgagcat
     3301 gttgtggacc gcacaggacg cgcaggccca gcccgacgtc cctcccgacg acatcgtcac
     3361 tcgcccgctc gtgctggagc cgggcgccgt cgatgtccgg ctcacgctgg cgatcaacat
     3421 ccagcagcgt gcgatcaccg aaccgaccgc cctgtcgccc gacgcgtggt ggggagtcct
     3481 gccacgcctg acgatcggcc tcattcacag tgacgccagc ctagaccaga tcgcgaccag
     3541 cggctcgttc tgcgtcaaga ccagcacctc gacgtgcgct cacctgtaca agggcagcgg
     3601 aatcgacgtg cggtacagcg cgctcgaggg gcaattcgcc attgcccccc ggctgcgcgc
     3661 ggtgatccgc gataccgatc cgttcaagcc ggcggtgacg ctgggcgcac ggctgcgctg
     3721 ggcgcacggc cggttcgcga tcaccggcga tccgtacctg cgcgtgccgc tcgccaacgc
     3781 ctcgctcggc aactacacgg agatcaacct cccggtgtgg ctcgcggtgc agcctcgcgc
     3841 cggctggatg atcgcgctgc gcggcggctt cgaatccgat ctgaatgtcg tccgcgatgg
     3901 cggccacatc ccgttcgcgc tggacgtcgc cgcgcgcgcg accgaccatg tcgacctcgg
     3961 cctcgagggc ggctggggca gcctgctcgg cccgctgcac gacgcccggc acgcgacgat
     4021 catgctcgcg gccggctggc acggcgtgcc gtagcgcccg atcgcagcga tcgctcaggc
     4081 gctgcgcagc ttggagtggt actggccgta ggcgaagtag atgacgaggc cgatcgccag
     4141 ccagacgccg agtcgcagcc agttggtcca accgaggccg aagatcatca gcatgttcgc
     4201 cgcgatgccg aggatcggca ccaccgggac cagcggcgtc ttgaactggc gcggcgcgtg
     4261 gggatcgcgg acccgcatga tccacacgcc gatgcacacc agcacgaacg cgaacagggt
     4321 gccgatcgac gtcatctcgc cgacgatgtc ctcgggcagg aacgctgcga acacgccgac
     4381 gaacacgaag aacaacatgt tcgacttcca cggcgtgcgg tatttggggt ggacatcgct
     4441 gaacaccttg ggcaccaggc cgtcgcggct catcgagaag aacacgcgcg actggcccat
     4501 cagcatgacc aggatcaccg acgagaaccc ggcgaggatc gcgaccgtga ccagcttggc
     4561 gagccaggcg taccccggca tgtgcttggt gatcgcgtag gcgaccgagg cctcgcgccc
     4621 cgcggtgcgg aagtcctcga ccgtcgcgat cccggtcagc acgtgcgcga acaggatgta
     4681 cagcacggtg cagatcgcca gggacccgag gatgccgatc ggcatgtc
//
LOCUS       DASVOR010002527         3048 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_144843,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002527 DASVOR010000000
VERSION     DASVOR010002527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144843"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1066)
                     /locus_tag="VF516_46430"
     CDS             complement(50..1066)
                     /locus_tag="VF516_46430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115275.1"
                     /translation="MTREVHMRSVAFVLMTPLLACTGYVQGGDSPLPDGATAPDAGGP
                     LPPPAHGFHVTSKPPPDIAPGEESTYCYYFRTSNTDELSVQRWVSRMDPGVHEMILYL
                     TPTDRQTPETISSTNCGIASSNGSPLWTYSAQTPDAHWDLPSNDGAGNPVGQLIRANQ
                     PAFLQIHYLNTTGSVIHPQVELNAYAYDNSIPVTLAAPFVTFRSGFQIAPGSEAQPAM
                     AETNGSCPLPVDTTGAPVKFFMMTTHTYRQGVHTSVSDGITTLFDSTSWMLPGATTWS
                     APFYAFQGTKLGYQCRYLNENTYTIMTGDNPTTDELCMSISFYVPADRGAIGHFCLDN
                     SMVY"
     gene            1195..2505
                     /gene="gorA"
                     /locus_tag="VF516_46435"
     CDS             1195..2505
                     /gene="gorA"
                     /locus_tag="VF516_46435"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEX8115276.1"
                     /translation="MARFDVDFFVIGGGSGGVRAARVAAEHGARVAIAEADRWGGTCV
                     IRGCVPKKLLVYASEISRTLQDARGQGWNIPESRHDWAALIAAKDEEITRLSNAYVER
                     LRKAGADVIAGRARLVDRHTIEVAGRTITAAHVLIATGGRARRPSAEWITSDDAFHLP
                     ELPARIAIMGGGYIAVEFAHIFAGLGAKVTLVHRDPLVLRGFDPDVRQAVTTNLSAHG
                     IDFICCRELVRRGRQVLAGGREIEVDVAMAAIGRDPATAGLGLTEAGVQLDARGAITV
                     DEWSRTSVPHIHAVGDVTGRVALTPVAIREGHAVADTLFGNRPTPIHHHLIPTAVFAQ
                     PSAAAVGLSEPAALAAGHDVVVFRARFRPMRYALSKRDEQILIKVVVDRATDRVLGVH
                     MVGGDAPEIIQTVAIAVTMGATKADLDRTFALHPTTAEELVLLR"
     gene            2735..>3048
                     /locus_tag="VF516_46440"
     CDS             2735..>3048
                     /locus_tag="VF516_46440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEX8115277.1"
                     /translation="MPSGGAPDIERALGLSHTATAVVLFLVPGVVQLVLDPVWMLLSE
                     RLGRPWLIRGGLAVMAAMSLVAAAAPGPVTLATALSIWGVATGAATSCSELTLIDRRE
                     TGG"
BASE COUNT          415 a          992 c         1163 g          478 t
ORIGIN      
        1 cgaggagatc gtgatcgaga tcgacgactg atcgccgcgc cggcggcgct cagtacacca
       61 tcgagttgtc gaggcagaag tggccgatcg cgccgcggtc cgccggcacg tagaagctga
      121 tcgacatgca cagctcgtcg gtcgtcgggt tgtcgccggt catgatggtg taggtgttct
      181 cgttgaggta ccggcactgg tagccgagct tcgtgccctg gaacgcgtag aacggcgcgg
      241 accaggtcgt ggcgccgggc agcatccagc tggtgctgtc gaacagcgtc gtgatgccgt
      301 cgctgacgga ggtgtgcacg ccttgccggt aggtgtgggt cgtcatcatg aagaacttga
      361 ccggcgcccc ggtcgtgtcg accggcaggg ggcagctgcc gttggtctcg gccatcgccg
      421 gctgcgcctc gctgcccgga gcgatctgga accccgatcg gaacgtgacg aacggggcgg
      481 ccagggtgac ggggatcgag ttgtcgtagg cgtacgcgtt gagctcgacc tgggggtgga
      541 tgacgctgcc ggtggtgttg agatagtgga tctggagaaa ggccggctga ttggcccgga
      601 tcagctggcc gaccgggttg ccggcgccgt cattgctcgg caggtcccag tgggcgtcgg
      661 gggtctgtgc cgagtaggtc cagagcgggc tgccgttgct gctggcgatc ccgcagttgg
      721 tcgacgatat cgtctccggc gtctgccggt cggtcggtgt caggtacagg atcatctcgt
      781 ggacccccgg gtccatgcgg gagacccagc gctggaccga gagctcgtcg gtgttcgacg
      841 tgcggaagta gtagcagtag gtgctctcct cgccgggggc gatgtcgggc gggggcttgc
      901 tcgtcacgtg gaagccgtgc gccggcggcg gcagcgggcc cccggcgtcg ggcgcggtcg
      961 cgccgtccgg aagcggcgag tcaccgccct gcacgtatcc ggtacaggcc aacagcggag
     1021 tcatgagcac gaacgcgacg cttcgcatgt gcacctcccg ggtcacgacc agggtatcaa
     1081 agccggctcg tcgagcgcac gcgatcgacg tcgaagaacg gttttcgccc gtcgaggtcg
     1141 tgacaggtgt ctcggcaggt gccgttgtgc tccgaggcac gtacactgcg ggccatggca
     1201 cggttcgacg tcgatttctt cgtgatcgga ggcggctccg gcggtgtgcg cgccgcacgc
     1261 gtggccgcgg agcatggcgc gcgggtcgcc atcgccgagg cggatcgctg gggtggcacg
     1321 tgcgtgatcc gggggtgtgt gcccaagaag ctgcttgtgt acgcaagtga gatcagccgc
     1381 acactgcagg acgcgcgcgg ccaggggtgg aacattccag aatcacggca cgactgggcc
     1441 gcactcatcg ccgccaagga cgaggagatc acgcggctgt cgaatgcata tgtggagcgg
     1501 ctgcgcaagg cgggcgccga cgtcatcgcc gggcgggccc ggctggtcga tcgccatacg
     1561 atcgaggtcg cggggcggac gatcaccgcg gcgcacgtgc tgatcgccac cggcggccgg
     1621 gcgcggcggc cgagcgcgga gtggatcaca tcggacgacg cgttccacct gccggagctg
     1681 ccggcgcgga tcgcgatcat gggcggcggc tacatcgccg tcgagttcgc gcacatcttc
     1741 gccgggctcg gcgcgaaggt caccctggtc catcgcgacc cgctggtgct gcgcggcttc
     1801 gaccccgatg tccgccaggc ggtcaccacc aacctgtcgg cgcacggcat cgacttcatc
     1861 tgctgccgcg agctggtgcg gcgggggcgc caggtgctcg cgggcgggcg cgagatcgag
     1921 gtcgacgtcg cgatggcggc gatcggccgc gatccggcga ccgcggggct cgggctgacc
     1981 gaggccggcg tacagctcga cgcgcgcggc gcgatcacgg tcgacgagtg gtcgcggacc
     2041 agcgtcccgc acatccacgc agtcggcgac gtcaccgggc gggtcgcgct caccccggtc
     2101 gcgatccgcg agggccacgc ggtcgccgac acgctgttcg gcaaccggcc gacgccgatc
     2161 caccatcacc tgatcccgac cgcggtgttc gcgcagccgt cggccgccgc ggtcgggctg
     2221 agcgagcccg cggcgctggc ggccggccac gacgtcgtcg tgttccgcgc ccggttccgg
     2281 ccgatgcgct acgcgctgag caagcgcgac gagcagatcc tgatcaaggt cgtggtcgac
     2341 cgcgcgaccg accgcgtgct cggcgtgcac atggtcgggg gcgacgcgcc cgagatcatc
     2401 cagaccgtcg cgatcgccgt gacgatgggt gcgaccaagg ccgacctcga tcgaacgttc
     2461 gcgctgcacc cgaccacggc cgaggagctg gtgctgctgc gctgagggcg gcggctgacg
     2521 cggctcgcca tgccgcggaa cgccgcgggc agcgattccg gaatgccggc cggctcgctc
     2581 cggttatgct cgcaccatgg cgaaccgctt caccgagcgc atctgatcgt cgatccgggc
     2641 ggcggcccgg gccgccgggg cgaggctccg gcggctgacc tccatcgccg cgctgctgtt
     2701 cggggtcgct ctgctcgacg agctgtcctc cggcgtgccg tccggaggcg cgcccgacat
     2761 cgagcgcgcg ctcggcctgt cgcacaccgc gaccgccgtg gtgctgttcc tcgtgccggg
     2821 agtggtgcag ctcgtcctcg atccggtgtg gatgctgctg tcggagcggc tcggccggcc
     2881 gtggttgatc cgcggcgggc tcgcggtcat ggcggcgatg tcgctggtgg cggccgccgc
     2941 gccggggccg gtgacgctgg cgaccgcgct gtcgatctgg ggcgtcgcga ccggcgccgc
     3001 cacgtcgtgc agcgagctga cgctgatcga ccgaagggag acgggggg
//
LOCUS       DASVOR010002528         1666 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_320599,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002528 DASVOR010000000
VERSION     DASVOR010002528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320599"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1226)
                     /locus_tag="VF516_46445"
     CDS             complement(129..1226)
                     /locus_tag="VF516_46445"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEX8115278.1"
                     /translation="MNTLRDLYPPLATAGERPGGLVPDTAGSHAAHGDGAPPVDVNRL
                     ADEVRQSSAFLDDVFAEVARVVVGQREMVERVLIGLLTGGHCLIEGVPGLAKTLLVKT
                     VAQTIHATFQRIQFTPDLLPADITGTIIYNMHTGEFTQKRGPLFANLVLADEVNRAPA
                     KVQSALLEAMQERQVTIGDTSHRLPDPFLVLATQNPIEQEGTFPLPEAQLDRFMLLIK
                     VSYPSASDERAMLDRMAVLDTPVALPVAAIDQVRASRRVVGSIYIDDRVRDYIVHVVQ
                     ATRRPRDYGLGELAPLIEYGASPRATLCLAAAARAHAFIRHRAYVTPDDIKAIAMDVM
                     RHRVVVTYEAEAEEVNSEDVVRRIFEYLPVP"
     gene            1299..1577
                     /locus_tag="VF516_46450"
     CDS             1299..1577
                     /locus_tag="VF516_46450"
                     /inference="COORDINATES: protein motif:HMM:NF020473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1844 domain-containing protein"
                     /protein_id="HEX8115279.1"
                     /translation="MAEQPHVLGPVDFSTHVLSLASTALIALGKMPGPDGQAHPLDLE
                     TARHLIDVLGMLEHKTQGNLDEPEQKLLRSLIYDLRVAYVDAQKATSG"
BASE COUNT          243 a          590 c          585 g          248 t
ORIGIN      
        1 ggagaagatc aggccgcggc ccatgaacac ggagtggtac tgacccgcga gctgctcgtc
       61 gaccagacgt ctcgcggcga tctcgatctt cttcgccttc ttgaacagct cggcggtgct
      121 gatcgccatc agggcaccgg caggtactcg aagatccgcc ggaccacgtc ctccgagttg
      181 acctcctcgg cctcggcctc gtaggtcacc accacgcggt gccgcatgac gtccatcgcg
      241 atcgccttga tgtcgtcggg cgtgacgtag gcgcggtggc ggatgaacgc gtgcgcgcgc
      301 gccgcggcgg ccaggcacag cgtggcgcgc ggcgacgcgc cgtactcgat cagcggcgcg
      361 agctcgccca ggccgtagtc gcgcggccgc cgggtcgcct ggaccacgtg cacgatgtag
      421 tcgcgcacgc gatcgtcgat gtagatcgag ccgaccaccc ggcgcgacgc gcggacctga
      481 tcgatcgccg cgaccggcag cgccaccggg gtgtcgagca ccgccatccg gtcgagcatc
      541 gcgcgctcgt cgctcgcgct cgggtagctg accttgatca gcagcatgaa gcggtcgagc
      601 tgggcctcgg gcagcgggaa cgtgccctcc tgctcgatcg ggttctgggt cgcgagcacg
      661 aggaacggat ccggcagccg gtggctggtg tcgccgatcg tgacctggcg ctcctgcatc
      721 gcctcgagca gcgccgactg gaccttggcc ggcgcgcggt tgacctcgtc ggcgagcacc
      781 aggttggcga acagcgggcc gcgcttctgc gtgaactcgc cggtgtgcat gttgtagatg
      841 atcgtgccgg tgatgtcggc cgggagcagg tcgggggtga actggatgcg ctggaacgtc
      901 gcgtggatgg tctgcgccac ggtcttgacc agcagcgtct tggccagccc ggggacgccc
      961 tcgatcaggc agtgaccgcc ggtcagcagg ccgatcagca cgcgctcgac catctcgcgc
     1021 tggccgacca ccacgcgcgc gacctcggcg aacacgtcgt cgaggaacgc gctcgactgg
     1081 cggacctcgt cggcgagccg gttgacatcg accggcggcg cgccatcgcc gtgcgccgcg
     1141 tggctccccg cggtgtccgg gaccagcccg ccggggcgct cgccggccgt cgcgagcggt
     1201 ggatacagat cgcgcaacgt gttcaccccc ctgacgatgt cacgaatcgc cgcccggcgc
     1261 ccgtgccgcg gatcaagccg gcggtatatg ttcgcgtcat ggcggaacag ccacacgtgc
     1321 tcggcccggt cgacttctcg acccacgtcc tgtctctcgc gtcgacggcg ctgatcgcgc
     1381 tcggcaagat gcccggaccc gacggccagg cccacccgct ggacctcgag accgcgcgcc
     1441 acctgatcga tgtgctcggc atgctggagc acaagaccca gggcaacctc gacgagccgg
     1501 agcagaagct gctgaggagc ctgatctacg acctccgcgt cgcgtacgtc gacgcgcaga
     1561 aggcgacctc ggggtgacgc ggcgcacggc gccgtcgagc ccgtcggtcg cctgggccgc
     1621 gatcgcctgc gccggcctcg ccggctgccg agagcgtgcg cccgac
//
LOCUS       DASVOR010002529         2124 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_58143,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002529 DASVOR010000000
VERSION     DASVOR010002529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58143"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            679..>2124
                     /locus_tag="VF516_46455"
     CDS             679..>2124
                     /locus_tag="VF516_46455"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8115280.1"
                     /translation="MDCDDVMLLVAVGVHSRTLSGSEEEQLHEHLASCEACRALTHDT
                     EHDRPRRLARSSTEPVNAADLPGLPAADPGRFARGDVLARGGMGRITRARDRRLGREV
                     ALKEVLAPELRARFEREAMITARLQHPAIVPIYEAGTWPDGSVFYTMRLVTGGTLGDA
                     IERAATLEQRLALLPHVIALTEALAYAHARRVVHRDLKPANVLVDEFGETVVIDWGLA
                     KELDREGPEAVASGAAAERPELTRAGAVVGTPCFLAPEQAAGEPVDERADVYSLGAIL
                     YHLLAGHPPYWDSVEHSADRLIAAAVQQPPTPIAKIAPRAPADLRAIVERAMASDKAA
                     RFPTAKDMAEELRRFETGQLLRSREYRLRELLVRRIRQHRAAVTVGAIAVCVLAAGGV
                     VSVRQIVARARETRRALAEAQLERGRQLVVDSDRGQAAAYLAAALTELPEDPVALRLA
                     TIALRDVDRRLGSFPGSAAAFRADGGELAIGQ"
BASE COUNT          294 a          740 c          804 g          286 t
ORIGIN      
        1 ccgggcaccc cgccggtcga cgccggcacc ggcggcggcg ccctgttcgc gcccgtgttc
       61 agcgcggtcg cgttcggcga cctcgagccc atcggccccg caccccgcgc tgcagctcgc
      121 cgtgctcacc gacgccaccg acgtcgccgc cacgctagcc gagatcggcg aacgttggcg
      181 tggccttgcg cgccacggcg atcagagtgc accggggaag ctgccgcgac gcgccgcctt
      241 gctgcattcc gccttcagag acgatccccg gaccgacgtg cgcgcggatg agacctcgtg
      301 caggtgtcgc ggcaacggga gcctgcggtc ctgcgaaagt catgcgcttc gtgcttccca
      361 tatgtccgga agtcattcgc cttgagggcc ccaccgcgtt gcttcgtgga gacgatttca
      421 cgtcggtcgc agaggaagcc ttaatcggta ccacgtcagc agcgcctggc cggaattgaa
      481 atagccgcct gatttcacga cctgatcgat agttcttcgt cgcactcagc tacgtgtctc
      541 gcccgcgacc ccgtcgacca gcggcagcca gcaatcaggc cgcgtcgtgc ttcgagtagc
      601 ggctcgaagc acgcccgata ccgcagatcg aagcaggcgc gcagttgcga cggttgccgc
      661 gccgcgacat gatgaggcgt ggactgtgac gacgtcatgc tcctcgtcgc cgtgggcgtg
      721 cacagccgca cgctgtccgg cagcgaggag gagcagctcc acgagcacct cgcgagctgc
      781 gaggcgtgcc gcgcgctgac ccacgatacg gagcacgacc ggccgcgccg gctcgcccgg
      841 agctcgacgg agccggtcaa tgcagccgat ctgcccgggc tgccggcggc ggatccgggg
      901 cggtttgcga ggggcgacgt gctcgcgcgc ggcgggatgg gccggatcac gcgcgcccgc
      961 gaccggcggc tcggacgcga ggtggcgctc aaggaggtgc tcgcgccgga gctgcgggcg
     1021 cggttcgagc gcgaggcgat gatcaccgcg cggctgcagc atccggcgat cgtgccgatc
     1081 tacgaggcgg ggacctggcc cgacggctcg gtgttctaca cgatgcggct ggtcaccgga
     1141 ggcacgctgg gcgacgcgat cgaacgggcg gcgacgctcg agcagcggct cgcgctgctg
     1201 ccgcatgtca tcgcgctgac cgaggcgctg gcgtatgcgc acgcgcggcg ggtggttcac
     1261 cgcgacctga agcccgccaa cgtgctggtg gacgagttcg gcgagacggt ggtgatcgac
     1321 tgggggctcg ccaaggagct ggatcgcgag ggccccgagg cggtcgccag cggcgcagcg
     1381 gcggagcggc cggagctgac ccgcgcgggc gcggtggtcg ggacgccgtg cttcctggcg
     1441 ccggagcagg cggccggcga gccggtcgac gagcgcgcgg acgtgtactc gctcggcgcg
     1501 atcctctatc acctgctcgc cggccatccg ccgtactggg acagcgtcga gcactcggcc
     1561 gaccgtctga tcgccgccgc ggtacagcag ccgccgacgc cgatcgcgaa gatcgcgccg
     1621 cgcgcaccgg ccgacctgcg ggcgatcgtc gagcgcgcga tggcgagcga caaggcggcg
     1681 cggttcccga cggcgaagga catggccgag gagctgcgcc ggttcgagac cggccagctg
     1741 ctgcgctcgc gcgagtaccg gctgcgcgag ctgctggtgc gacggatccg ccagcaccgc
     1801 gcggcggtga cggtcggcgc gatcgcggtc tgcgtgctcg cggccggcgg cgtggtcagc
     1861 gtgcggcaga tcgtggcgcg agcgcgcgag acccggcggg cactcgcgga ggcgcagctc
     1921 gagcgcggcc ggcagctggt cgtcgacagc gaccgcgggc aggcagccgc gtacctggcc
     1981 gctgcgctca cggagctgcc agaggacccg gtcgcgctgc ggctcgcgac gatcgcgctg
     2041 cgcgacgtcg atcgccggct gggcagcttt cccggcagcg cggcggcgtt tcgcgcagac
     2101 ggcggcgagc tggcgatcgg tcag
//
LOCUS       DASVOR010002530         2107 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_378935,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002530 DASVOR010000000
VERSION     DASVOR010002530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2107
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_378935"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1900
                     /locus_tag="VF516_46460"
     CDS             <1..1900
                     /locus_tag="VF516_46460"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEX8115281.1"
                     /translation="APSAMTFRWTATSGSVGDAAATAPSFTCTKVGRATITVAVSDGD
                     PACATQQSVDVVCTEPAPQTPIKHVIVLIGENRTFDHTFGTYVPRDGQIVSNLLSKGI
                     INADGTPGPNFALAAQAQAAPQGAFYIAPDSKTPYLVLPPPSTSGAPTAQRTTAPPFQ
                     TVDQAALESDVAPEDLVLLTTGATGLPARVVDTRVTNAAALPPGPFQMTGPTLPYDAY
                     TGDTIHRFYQMWQQTDCSVANATAANPSGCLSDLFPFVAVSFSTADNGVGSSMAFFNV
                     NHGDVPFLRMLADNFAMSDNMHQAVQGGTGANHSLLGFGDAVAWTDGHGNPALPPASA
                     IANPNPRAGTNNRYTLDGNFSSCADITQPGVGAIVSYLAQLPHKPNPNCAPGTYYYLN
                     NTNPAYNPDGSLKTTGTFVPPTIQRSIGDSLSEKGISWRFYGGGLARGTGYCQICNPF
                     EYQTQFMADPAIRNEHLKDTVDMFSDIASGTLPSVSFAHPDGALDGHPSSSKLGLFEA
                     YARNILVKLHASPTLEASTAVIITFDEGGGYYDSGFIQPIDFFGDGPRIPLIIVSPFT
                     TGGKIDHGYADHVSILKFIERNWQLAPITSRSRDNLPNPIVNATNPYVPTNMPALTDL
                     FDLFDFTH"
BASE COUNT          357 a          817 c          660 g          273 t
ORIGIN      
        1 cgcgccgtcg gcgatgacct tccgctggac cgcgacgtcg ggcagcgtcg gcgacgccgc
       61 cgcgaccgcc ccgagcttca cctgcaccaa ggtcggccgc gcgacgatca ccgtggcggt
      121 gtccgacggc gaccccgcgt gcgcgaccca gcagtcggtc gacgtggtgt gcaccgagcc
      181 ggcgccgcag accccgatca agcacgtgat cgtgctgatc ggcgagaacc gcaccttcga
      241 tcacacgttc ggcacctacg tgccgcgcga cggccagatc gtctccaacc tgttgtcgaa
      301 gggcatcatc aacgccgacg gcacgccggg cccgaacttc gcgctcgccg cccaggccca
      361 ggccgcgccg cagggcgcgt tctacatcgc gccggacagc aagacgccgt acctcgtgct
      421 gccgccgccg agcacgtcgg gcgcgccgac cgcgcagcgg accaccgcac cgccgttcca
      481 gaccgtcgac caggcggcgc tggagtccga cgtcgctccc gaggatctcg tgctgctcac
      541 caccggcgcg accgggctgc cggcccgcgt ggtcgacacc cgcgtcacca acgccgccgc
      601 gctgccgccc gggccgttcc agatgaccgg gccgaccctg ccgtacgacg cgtacaccgg
      661 cgacacgatc catcgcttct accagatgtg gcagcagacc gactgctcgg tggccaacgc
      721 gaccgcggcc aacccgagcg gctgcctgag cgacctgttc ccgttcgtgg cggtctcgtt
      781 ctcgaccgcc gacaacggcg tcggcagctc gatggcgttc ttcaacgtca accacggcga
      841 cgtgccgttc ctgaggatgc tcgccgacaa cttcgcgatg agcgacaaca tgcaccaggc
      901 ggtgcagggc ggcaccggcg ccaaccactc gctgctcggg ttcggcgacg cggtcgcgtg
      961 gaccgacggc cacggcaacc ccgcgctgcc gccggcgagc gcgatcgcca accccaaccc
     1021 gcgggccggc accaacaacc gctacacgct cgacggcaac ttcagcagct gcgcggacat
     1081 cacgcagccc ggcgtcggcg cgatcgtcag ctacctcgcg cagctgccgc acaagccgaa
     1141 cccgaactgc gcgcccggca cctactacta cctcaacaac accaacccgg cctacaaccc
     1201 ggacggctcg ctcaagacca ccggcacgtt cgtgccgccg acgatccagc gctcgatcgg
     1261 tgactcgctg agcgagaagg gcatctcgtg gcggttctac ggcggcgggc tcgcgcgcgg
     1321 caccggctac tgccagatct gcaacccgtt cgagtaccag acccagttca tggccgaccc
     1381 ggcgatccgc aacgagcacc tcaaggacac cgtcgacatg ttcagcgaca tcgccagcgg
     1441 cacgctgccg tcggtgtcgt tcgcccaccc cgacggcgcg ctcgacggcc acccgtcgtc
     1501 gtcgaagctc ggcctgttcg aggcctacgc caggaacatc ctggtcaagc tccacgccag
     1561 cccgacgctc gaggccagca cggcggtgat catcacgttc gacgaaggcg gcggctacta
     1621 cgactcgggc ttcatccagc cgatcgactt cttcggcgac ggcccgcgca tcccgctgat
     1681 catcgtctcg ccgttcacca ccggcggcaa gatcgaccac ggctacgccg accacgtgtc
     1741 gatcctgaag ttcatcgagc gcaactggca gctcgcgccg atcaccagcc gcagccgcga
     1801 caacctgccg aacccgatcg tcaacgcgac caacccgtac gtgccgacca acatgccggc
     1861 gctgaccgat ctgttcgacc tgttcgactt cacccactag cccagcgtct cgatcggatc
     1921 tcggccgcgg gctcagcgct ggaccagcag gccctcggcg agcagcagca gcagcaccgc
     1981 ggcagcgagc gcgtgccaca gctcgacgcg gcggcgggtg tcggccggcg ctgcggagga
     2041 cccggtgccc gagacgggca gcgcggaggg cggcgcggga gtgagatcgg agccgtgggg
     2101 atcgagg
//
LOCUS       DASVOR010002531         1936 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_203486,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002531 DASVOR010000000
VERSION     DASVOR010002531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_203486"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="VF516_46465"
     CDS             <1..474
                     /locus_tag="VF516_46465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115282.1"
                     /translation="ARTMARPDDGAAIDELSRRLGAAGEKDWAIEIAVRGSERARSSP
                     TRWRALLAASVAYVDRRDVVPALDYANRALSACGDHPEACPSWERIRTELYQEQLDAG
                     VKSGIDPRRDPEAAQAFRHAGERALRQIHLGGRGGRGATGAPAPGSTDRSTGNHP"
     gene            522..1664
                     /locus_tag="VF516_46470"
     CDS             522..1664
                     /locus_tag="VF516_46470"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEX8115283.1"
                     /translation="MRRVRVVAVLVLVSACAETMSSSPPHTRLTAKDIVQRASPAIVR
                     IEAGAEKIGTGFIVDKAGVVATNLHVVAGESAIRVKLSDGSAYAVTQIDGVDPARDLA
                     LLQIRAGKDLPTLKLGDSSAMSAGDQVVAIGNPLGFDNSVTSGLISQVRPICGPAEIA
                     ASKCNQELTVLQISAAISPGSSGGPLFNQYGEVVGVTTAIITAGQNFNLAVPGNYLKP
                     LVSQRIALSTDEFAKQTQEADEHRHRERAETKPSGEIPRHIPIHEITVFDGCKVEDIE
                     DIINQIGEAIETGAPLYNQGNHEACFRIYEGTAVKFEHDGPCAGVRSAFGDGLLRASG
                     LNTYTEKAWAMRDTFDGLLDAARRWAEKHPQPAPGGRSAPAPTKKK"
     gene            1661..>1936
                     /locus_tag="VF516_46475"
     CDS             1661..>1936
                     /locus_tag="VF516_46475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115284.1"
                     /translation="MILYLFDIDGTLLHARRSGSSSFDAVFADHHGVAGACQGVRFGG
                     KTDPALIEEIFVARLGRSPSEAERAAFLDAYLARLRAHLERHGVEVLA"
BASE COUNT          301 a          693 c          688 g          254 t
ORIGIN      
        1 gcccggacga tggcgcgccc ggacgacggc gcggcgatcg acgagctgtc gcgccggctc
       61 ggcgccgcgg gcgagaagga ctgggcgatc gagatcgcgg tccgcggcag cgagcgcgcg
      121 cggagctcgc cgacccgctg gcgcgcgctg ctcgccgcgt cggtcgcgta cgtcgatcgg
      181 cgcgacgtgg tgccggcgct cgactatgcc aatcgcgcgc tgtcggcgtg cggcgatcat
      241 cccgaggcct gcccgagctg ggagcggatc cgcaccgagc tgtaccagga gcagctggac
      301 gccggcgtga agtcgggcat cgatccgcgt cgcgaccccg aggctgccca ggcgttccgc
      361 catgccggcg agcgtgcgct gcgtcagatc catctcggcg gccgcggcgg gcggggcgcg
      421 acaggggcgc ccgcgccggg cagcacggac cgttccactg gaaaccatcc gtaacaccgc
      481 ccagctgtgc atcagcgcag gtcccgggct atgatcccgc catgcgccgt gtccgcgtgg
      541 tcgccgtcct ggtactggtc agcgcctgtg ccgagacaat gtcgtccagt cccccccaca
      601 cgcggctgac ggccaaggac atcgtacagc gcgccagccc ggcgatcgtg cggatcgagg
      661 ccggggcgga gaagatcggc accggcttca tcgtcgacaa ggccggcgtg gtcgcgacca
      721 acctccatgt cgtggcgggc gagtcggcga tccgggtcaa gctgtccgac ggctcggcgt
      781 acgcggtgac ccagatcgac ggcgtcgacc ctgcgcgcga cctcgcgctg ctccagatcc
      841 gcgccggcaa ggacctgccg accctcaagc tcggcgacag cagcgcgatg tcggccggcg
      901 accaggtcgt ggcgatcggc aacccgctgg gcttcgacaa ctcggtcacc agcggcctga
      961 tctcgcaggt ccggccgatc tgcggcccgg ccgagatcgc cgcgagcaag tgcaaccagg
     1021 agctcaccgt gctgcagatc tcggcggcga tctcgccggg atcgagcggc ggcccgctgt
     1081 tcaaccagta cggcgaggtc gtcggggtga ccaccgcgat catcaccgcc gggcagaact
     1141 tcaacctcgc cgtgccgggc aactacttga agccgctggt ctcgcagcgc atcgcgctgt
     1201 cgaccgacga gttcgccaag cagacccagg aggccgacga gcaccgccac cgcgagcgcg
     1261 ccgagaccaa gccaagcggc gagatcccgc gccacatccc gatccacgag atcaccgtgt
     1321 tcgacggctg caaggtcgag gacatcgagg acatcatcaa ccagatcggc gaggcgatcg
     1381 agaccggcgc gccgctgtac aaccagggca atcacgaggc ctgcttccgg atctacgagg
     1441 gcacggcggt caagttcgag cacgacggcc cgtgcgccgg ggtgcgcagc gcgttcggcg
     1501 acgggctgtt gcgcgcgagc gggctcaata cctacaccga gaaggcgtgg gcgatgcgcg
     1561 acacgttcga cggcctgctc gacgccgcgc ggcggtgggc cgagaagcac ccgcagcccg
     1621 cgccgggcgg gcgctccgct ccggcaccca ccaagaagaa gtgatcctgt acctgttcga
     1681 catcgacggc acgctgctcc acgcccggcg gtccgggtcg agctcgttcg acgcggtgtt
     1741 cgcggaccac catggcgtcg ccggtgcgtg ccagggcgtg cggttcggtg gcaagaccga
     1801 tccggcgctg atcgaggaga tcttcgtcgc gcgactcggc cggtcgccga gcgaggccga
     1861 gcgcgccgcg ttcctcgatg cctacctcgc gcggctgcgc gcccacctcg agcgccatgg
     1921 cgtggaggtc ctcgcc
//
LOCUS       DASVOR010002532         3256 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_466249,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002532 DASVOR010000000
VERSION     DASVOR010002532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466249"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1058)
                     /locus_tag="VF516_46480"
     CDS             complement(<1..1058)
                     /locus_tag="VF516_46480"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEX8115285.1"
                     /translation="MKLCPRCQKQFSDDANFCPVDAARLVPVDTQAAAVDALAAKFEL
                     GERLGGGRTGAVHRATDKATGQPAAVKLIAPAVVALPGLAQRLERELKQLERVASGGV
                     ARVLASGKRGDDSWVALELLDNVQTLGDAVAARGAMPIDKAAKLIEIIGEALIEAAQV
                     GVVHRDLAPKNIVFAGDDVKLINFSLPVPTSDKVPGVPGFVAPEQYDGKQVDQRSNLY
                     SLGALYYFVLTAQPVQSDANGLLVPPSALAEVPGPVEAVILRALDRSPTKRFLTVRQF
                     VDEVGRVARGEPGLRSTQPLGRAGKQPAEIVQTLVDAHPLALALAPAEAEGAGPLGTG
                     TILGLGTLKAPAAAPAPA"
     gene            1250..1408
                     /locus_tag="VF516_46485"
     CDS             1250..1408
                     /locus_tag="VF516_46485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115286.1"
                     /translation="MASRTAASRVKRKNKHEKAGRRRKNRLARKSTLSNAELFSTLGE
                     PGKPAPAK"
     gene            complement(1427..>3256)
                     /locus_tag="VF516_46490"
     CDS             complement(1427..>3256)
                     /locus_tag="VF516_46490"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEX8115287.1"
                     /translation="REEQQAENFRKMLLAMARDIRVILVKLCDRLDNMRSLHHLPPEK
                     QERIASETMQIYAPLANRLGIQWVKVELEDLAFKYLYPGEYEQLAAEVAKSRGERLEY
                     IHHVEKLIHKEMTEHGVPCEVMGRAKHLWSIWQKMKRTQRPFEEIHDAIGFRVITDTQ
                     MHCYQGLGVAHQTWTPIPGRFKDYIALPKPNLYQSLHTAVIGPRGERIEIQIRTREMN
                     LVAEHGIAAHWKYKEGSVASVDDKKFAWLRQLMENQKELRDPTEFLESVKIDLFGDEV
                     YVFTPQGDVKALPKGACPIDFAYAVHSNIGDHCSGSRVNGMIVPLRYQLRNGDTVEII
                     TSPNQKPNKDWLKLVKTARARTKIRYLLRTEQRDKAVVLGNDLLDKALRKHNTTLARA
                     EKQLAHAAKELRCVTVDELIIQVGYGKITTQQVLEKALPELTKKEHEPPARTESMLKT
                     LLRKVTRRTSTSGIKVAGEEDILVRFAKCCSPLPGDPIVGFITRGRGVTVHRRDCDKG
                     LDLDPDRRIDVEWDGNGKAQHEVAIQILCADKPGLLAHISQSFSDQGVNISQAHCRAT
                     DDGRAVNTFHASVRDLDQLKSVIRSLSRIKGVYSVDRVSADVN"
BASE COUNT          451 a         1101 c         1151 g          553 t
ORIGIN      
        1 ctcgccgggg ccggggccgc ggccggtgcc ttgagcgtgc cgaggccgag gatcgtcccg
       61 gtgcccagcg gcccggcgcc ctccgcctcg gccggggcca gagccagagc cagaggatgc
      121 gcatcgacca gggtctggac gatctcggcc ggttgcttgc cggcgcggcc gagcggctgg
      181 gtcgaccgca ggcccggctc gccgcgcgcg accctgccga cctcgtcgac gaactggcgc
      241 acggtcagga accgcttggt cgggctgcgg tcgagcgcgc gcaggatcac ggcctcgacc
      301 ggccccggca cctcggcgag cgccgacggc ggcaccagca ggccgttggc gtcggactgg
      361 accggctgcg cggtgagcac gaagtagtac agcgcgccga ggctgtacag gttggagcgc
      421 tggtcgacct gcttgccgtc gtactgctcg ggcgcgacga acccgggcac gccgggcacc
      481 ttgtcgctgg tcggcaccgg cagcgagaag ttgatcagct tgacgtcgtc gcccgcgaac
      541 acgatgttct tgggcgcgag gtcgcggtgg accacgccga cctgcgccgc ctcgatcagc
      601 gcctcgccga tgatctcgat cagcttggcg gccttgtcga tcggcatcgc gccgcgcgcc
      661 gcgaccgcgt cgcccagggt ctggacgttg tcgagcagct cgagcgcgac ccaggagtcg
      721 tcaccgcgct tgcccgacgc gagcacccgg gcgaccccac cgctggcgac ccgctcgagc
      781 tgcttgagct cgcgctcgag ccgctgcgcg agcccgggca gcgcgaccac cgccggggcg
      841 atcagcttga ccgcggcggg ctggccggtc gccttgtcgg tcgcccggtg caccgccccg
      901 gtccggcccc cgccgagccg ctcgccgagc tcgaacttgg cggcgagcgc gtcgaccgcc
      961 gcggcctggg tgtccacagg gacgagacgt gcagcgtcga ccgggcagaa gttggcatcg
     1021 tcgctgaact gcttctggca cctcgggcaa agcttcatgc ctcgttccct ctaactggcg
     1081 cggatcgtag ggagatgtgg tcgagaaagc aaacgatacg gccagcgcgc ccgtggccct
     1141 gggtgcggct tagccgacga gccgggggaa gtcgagcgac gggggccgct gtcggttgac
     1201 acgggcgcgg caggacgcta acaaccccgg tccacttcga ggaatcgcca tggcttcccg
     1261 caccgccgcc tccagggtca agcgcaagaa caagcacgag aaggccggcc gccgccgcaa
     1321 gaaccggctc gcgcgcaagt ccacgttgtc gaacgccgag ctgttcagca cactcggcga
     1381 gccgggcaag cccgcgccgg cgaagtgacg gtcgtttagt gtccggtcaa ttgacgtcgg
     1441 ccgacacgcg atcgaccgag tacacgccct tgatccgcga tagcgagcgg atcacgctct
     1501 tgagctgatc gaggtcgcgg accgaggcgt ggaacgtgtt gaccgcgcgg ccgtcgtcgg
     1561 tcgcccggca gtgcgcctgc gagatgttga cgccctgatc gctgaacgac tgcgagatgt
     1621 gggcgagcag ccccggcttg tcggcgcaca ggatctggat cgcgacctcg tgctgggcct
     1681 tgccgttgcc gtcccactcg acgtcgatcc ggcggtcggg atcgaggtcg aggcccttgt
     1741 cacagtcgcg gcggtgcacg gtgaccccgc ggccgcgcgt gatgaacccg acgatcggat
     1801 cgccgggcag cgggctgcag cacttggcga accggaccag gatgtcctcc tcgcccgcga
     1861 ccttgatgcc gctggtcgag gtccgccggg tgaccttgcg cagcagcgtc ttcagcatgc
     1921 tctcggtccg ggccggcggc tcgtgctcct tcttggtcag ctcgggcagc gccttctcca
     1981 gcacctgctg ggtcgtgatc ttgccgtagc cgacctggat gatcagctcg tcgacggtca
     2041 cgcagcgcag ctccttggcc gcgtgggcga gctgcttctc ggcgcgcgcc agcgtggtgt
     2101 tgtgcttgcg cagcgccttg tcgagcaggt cgttgccgag caccaccgcc ttgtcgcgct
     2161 gctcggtgcg caggaggtag cggatcttgg tccgcgcgcg cgcggtcttg accagcttga
     2221 gccagtcctt gttcggcttc tggttggggc tggtgatgat ctcgacggtg tcgccgttgc
     2281 gcagctggta gcgcagcggg acgatcatgc cgttgacccg cgagcccgag cagtggtcgc
     2341 cgatgttgga gtgcaccgcg tacgcgaagt cgatcgggca cgcacccttg ggcagcgcct
     2401 tgacgtcgcc ctgcggggtg aacacgtaga cctcgtcgcc gaacaggtcg atcttcaccg
     2461 actcgaggaa ctcggtcgga tcgcgcagct ccttctggtt ctccatcagc tggcgcagcc
     2521 aggcgaactt cttgtcgtcg accgacgcga ccgaaccctc cttgtacttc cagtgcgcgg
     2581 cgatgccgtg ctcggcgacc aggttcatct cgcgggtccg gatctggatc tcgatccgct
     2641 cgccgcgcgg cccgatcacg gcggtgtgca gcgactggta gaggttgggc ttgggcagcg
     2701 cgatgtagtc cttgaaccgg ccggggatcg gcgtccaggt ctggtgcgcg acgccgaggc
     2761 cctggtagca gtgcatctgg gtgtcggtga tcacgcggaa gccgatcgcg tcgtggatct
     2821 cctcgaacgg ccgctgcgtg cgcttcatct tctgccagat cgaccacagg tgcttggcgc
     2881 ggcccatgac ctcgcacggc acgccgtgct cggtcatctc cttgtggatc agcttctcga
     2941 cgtggtgaat gtactcgagc cgctcgccgc ggctcttggc gacctcggcg gcgagctgct
     3001 cgtactcgcc ggggtagagg tacttgaacg cgaggtcctc gagctcgacc ttgacccact
     3061 ggatgccgag ccggttggcg agcggcgcgt agatctgcat ggtctcggac gcgatccgct
     3121 cctgcttctc gggcggcagg tggtgcagcg agcgcatgtt gtcgagccgg tcgcacagct
     3181 tgaccaggat gacgcggatg tcgcgcgcca tcgcgagcag catcttgcgg aagttctcgg
     3241 cctgctgctc ctcgcg
//
LOCUS       DASVOR010002533         9504 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_144901,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002533 DASVOR010000000
VERSION     DASVOR010002533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 9504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9504
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144901"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..373)
                     /locus_tag="VF516_46495"
     CDS             complement(203..373)
                     /locus_tag="VF516_46495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115288.1"
                     /translation="MKKSTLKLAIRRETLRALAQLDLVRVAGGNPDAQQAGTVNPEDG
                     CPFPAAALPPKW"
     gene            complement(407..3904)
                     /locus_tag="VF516_46500"
     CDS             complement(407..3904)
                     /locus_tag="VF516_46500"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEX8115289.1"
                     /translation="MADHDLDLSGRTLGDFVIHSKIGEGGWGLVYRAEQKPLGREVVV
                     KVLHGERIEKGASVERFLREARLASQLGHPCATHVHAFGVHEDQGQRLWWIAMELVRG
                     VTLADWLKAHGPMPLEHLVPYFEYVAEVVYEAHNHGIIHRDLKSSNMMVIERNGRLFP
                     KLLDFGIAKVDPDVDPDVTLVDESEADRVPTSLIRPSPREGVIRPKTEAKEREQLTKR
                     GAAFGSAPYMAPEQWRDAAGVGPAADIYSLGVVIYEAATGRRPFTGKNTTEYRRLHGY
                     GEVPPVDMAAPSLWFGVSRELDRVLQRALSKYPESRQRSVLDLAADLRAVLRTSDREQ
                     LRAAAQQWEDRSHLPGLLWGDDVLADVGVVANAAAEGGLSELECSFVVASQRRARRRL
                     WVRRALVALAGLVAVGVFLYRGVMQARMNERIGIESHVEQGRQALLHSESSEAVRHLE
                     QAYQRGEHSPGVTFMLARALQPHMSELARFASNAGRVWSAAFAPDGKRVLTTDDKSAR
                     MWDASSGQLLFTMNHGDTVYGAVFSPDGARIITAGGDGTVRIWSAATGAAIRELRYQG
                     SGSNRWRYSEVAMSSHFVAALDKRGRTAHVWDAETGTPVAELVNEAPERKLLAFSADG
                     HWLALSSRDEVRLFATSTWQRAVTIAEPHVRSLSFDPTGPRLAVGTYNGVASIWEVAT
                     GVRTRSLREAGASVDAVAFSRDGVLVATASRDGMEQVWDASSGGLRIQSNSHHDKISA
                     IEFSPSGDLMLSAGADGAVVVWNVATGMPVARLEGPKAFISAAHFDPEARRVVGSSWD
                     GTAWVWDAASPYRRWDSPQIGTDCDTEDSLVPDQRFIALSCRNHGTHVWDTARGKLLA
                     ELPGVTTVEGSDTSVFPALTTSGDQAAIARGNTVELYALPSGQLLRTIVHTAAVSAVA
                     FAPTGHDLISGAVDGSLLITHDESDPITLQRSSAAIDAVAILTDGRVVATDASERLRV
                     IGQDRNVLLKELAAPSRIKLLRPSLDGRRLVTISIATDAPPVLWDLEQYRLVARLDSQ
                     VGRVFTARFVASGHEILTAGADGAAWLWDATTGRPRQRFRGDSHFLVDAVLAPDGSMV
                     VAGGSDGFLRFWDTSDGHLLWTLQVHTSYVIGAHYEGNDIVTRGFAGDIARWALPPPD
                     KIIEVCHASTCTPMTTAEK"
     gene            4718..5758
                     /locus_tag="VF516_46505"
     CDS             4718..5758
                     /locus_tag="VF516_46505"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEX8115290.1"
                     /translation="MGCDAGPDPTVAIGENNVEGDWELRLGEISIGGSRQCTISIPDR
                     GMSARHCLLDRRARARKLLLYDLDSSHGTFFREQKLEGSVELRPGDMFTAPPVTFVCL
                     SEAMRQHRPTVFEILGSDAARPPDWVVVQAATGSGPMLLTGEAGCDLDRLASAIHGMT
                     SRRLRSPIELAAIPHDHAARTDLIQQASKTSLILALDDESEPLPPDVVSKLFDARLGV
                     RFIAIASSPNIARRVLTGAKVEFMQHVPVRPLADRKDEIRELLDRQFAKRGFCRRVND
                     LTPVNQDALKTYPWPGNFAELREVVDGIIAHAKLGGLRPAAESLGISHQKLDRRFRRV
                     GLKIRRLFGEGE"
     gene            5844..6851
                     /locus_tag="VF516_46510"
     CDS             5844..6851
                     /locus_tag="VF516_46510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115291.1"
                     /translation="MRAIELSREIDANWHCENTPCNASDFVFCGDVLDRPRQRHECAP
                     PSISDISGSTSAAFVHKRLRMLVQIRALREAHEQGLALDMAIAADGLATAADELAASV
                     RNLLARKARMETHVQHSRAYRLRRAHVDAQVQNALYEARALREWIAGLFETFLRPEPP
                     DPSSVWPDGRSLPESYPWLRLPEWIRSRREDEQHRRVLEAKAAEWVQSGGGQSRLLDA
                     DELREVRNWLSDEKARAPGVNEEMQSLLAHSDAALAAQVAEAEGRRRRLRRWVTIALV
                     VLTGAVLAVSTLAVIAVRRSRVADEQSQEARRQSQEVQRQSQEARRQLAATMLLGGTN
                     S"
     gene            complement(7028..8698)
                     /locus_tag="VF516_46515"
     CDS             complement(7028..8698)
                     /locus_tag="VF516_46515"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEX8115292.1"
                     /translation="MVTWATVAVMAATAFAVSVDGAASPLHAVGLVAIKSPVLAVGAL
                     LCAGVCAWSWRRRTLGLTWFELAVLGYLVAGALSVAMNRPALPFLSPWLALNFTALVM
                     VAVGSEASRQDRAQLLDGLLVVGVVLAALTIYESAGGFIPWDVGRRPGATFGNRNAVG
                     GFCAILIPIAIVRLWTRPTYLRGGALAVLLLSVLLCRARSAWMGLMVTGALYFLWQIK
                     RRGPRSDAPRPARRARLVALGAILAAVAALQFTPWIGLQWKESSPILSSFSRMLESQT
                     GTGRSRVDQHLVGITMLAERPLIGLGPGAWRQDAPRFVHQATGYHTGFIDALWTPASD
                     LVRQAVELGVIGLCAAASMLAALLLGARARIAAADDRVPLALAASLIVAVVISAFDAL
                     LYRPPSLALIAVIAGALRNEGKRLSYRVPPCLGMASLGICGTLCVAALVPRYLAMQAY
                     QRSFSAQTVIALADGQFLPAEALMAVRDARGKADCTVLHKAASLVDRYLPYEPENLEV
                     LAACAVERGHPDEAARLLERALRVEPHDEAAARELVRLRTGAARPASQ"
     gene            complement(8740..>9504)
                     /locus_tag="VF516_46520"
     CDS             complement(8740..>9504)
                     /locus_tag="VF516_46520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115293.1"
                     /translation="LAHAAPTPATTSALAAAAQALAGHGEPGGLWRFFGRAAQNPPPD
                     FDDTCCALVALHQARLTIETKVLALLARAAFPSGGYGTWVHPGLNDPRRVDAVVNANI
                     LYYLAVSGQPTGPLARFLVEFALQRQLSKLSAYALSDPPAIYTLMRAFCHGPAPALAP
                     LAARVPPHLLAHQAGDGSFGHELDTALSLTALLDAGHRGEGLEAAGRWLLRRQRPDGG
                     WPARTFFCDFQPTYYGSEELTTALCAEALAKLTAPT"
BASE COUNT         1636 a         3278 c         3105 g         1485 t
ORIGIN      
        1 cggccgagct gccggagcgg gtcgaggtcg cgctcggcgc ggacctggtg cgcggtgtcg
       61 gcggcgccac gctcgccgtg ttccgagctg ccagggccgc ggggctgcca gccgctggcc
      121 cggcggcgct ggcgtcgagc aggtcgaggt cacgctcgac gctcggcgcg aactggccga
      181 ccgggccggc ccggctatct acctaccact tgggtggtag cgcagccgcc gggaacgggc
      241 atccatcctc aggatttacc gtgcctgctt gctgtgcatc cgggttgccg ccggcgaccc
      301 gaacaagatc caattgagcg agggcacgga gcgtttcgcg acggatggcg agctttagcg
      361 ttgatttctt catcacgcta tgataacgcg cgcggagtta gcgcagctac ttttcggcgg
      421 ttgtcatcgg cgtacatgtg ctggcgtggc acacctcgat gatcttgtcc ggcggtggca
      481 gcgcccaacg tgcgatatct ccagcgaagc cccgcgtgac gatgtcgttg ccttcgtagt
      541 gcgcgccgat gacgtaggag gtgtgcacct gcagcgtcca gagcaagtgc ccatccgacg
      601 tgtcccagaa ccgcaggaac ccgtcgctgc cgcccgcaac aaccatcgat ccgtcgggag
      661 caagtaccgc atccacgagg aaatgtgagt ctcctcgaaa tctctggcgt gggcgtccgg
      721 ttgttgcgtc ccacagccac gccgctccat cggccccggc cgtgaggatc tcgtgtccgc
      781 tggcaacgaa gcgcgccgtg aacacccgtc ctacttggct atcaagccgc gctaccagcc
      841 ggtactgttc aagatcccat agcaccggcg gagcatcggt tgcgatcgat atggtgacca
      901 gccgtctgcc atcgagtgac ggccgcagca gcttgatacg agacggagct gcgagttcct
      961 tcagcagcac gttgcggtct tggcctatga cacgcagtcg ctcgctagca tcggttgcca
     1021 ccacgcgccc atcagtgagg atggccacag cgtcgatcgc ggcgctcgac ctctgcagcg
     1081 tgatcgggtc gctttcatcg tgcgtgatca ggagcgaacc atcgacggca ccgctgatta
     1141 ggtcatggcc cgtaggagcg aacgcaaccg cactgactgc cgccgtatgg acgatcgtgc
     1201 gaaggagctg gccgctgggg agcgcataga gctccacagt gttgccccgg gcgatcgccg
     1261 cttggtcgcc ggacgtagtc aacgcgggga acaccgaggt gtcgcttcct tcgacggtgg
     1321 tcacacccgg cagttccgcg agcagctttc cccgcgctgt gtcccagaca tgggtgccat
     1381 ggttccggca gctgagcgcg atgaaccgct gatcaggaac aagactatcc tcggtatcgc
     1441 agtccgtgcc gatctgcggg gagtcccagc ggcgatacgg cgaggccgca tcccacaccc
     1501 aggcggtgcc gtcccacgac gaaccgacga cacggcgcgc ctccggatcg aagtgagcgg
     1561 cgctgatgaa ggccttgggc ccttcgagtc tggcaacggg catccctgtg gcgacgttcc
     1621 agacgacgac tgcgccatct gcgccggcag agagcatgag gtcacccgaa ggagagaact
     1681 cgatcgcaga gatcttgtca tgatgcgagt tggactgaat gcgcagaccg cctgacgacg
     1741 catcccatac ctgttccatg ccatcacggc tcgccgtcgc aacgagcaca ccatcacgcg
     1801 aaaacgccac ggcatcgacc gacgctcctg cctcgcgcag ggatcgggtg cgcacgcccg
     1861 tcgcgacctc ccagatcgag gccacgccgt tgtaggtccc aaccgcaagc cgcgggccgg
     1921 ttggatcgaa gctcaggctg cgaacgtgtg gctcggcgat cgtgacggca cgctgccatg
     1981 tcgacgtagc gaacaagcgc acctcatcgc ggctggacaa cgcgagccag tgcccgtccg
     2041 cgctgaatgc cagcaatttc ctttccggcg cctcgttgac caactcggcg accggcgttc
     2101 ccgtctctgc gtcccagaca tgcgcggtcc ttcccctctt gtcgagagca gccacgaagt
     2161 gcgacgacat cgcgacctcc gagtaacgcc accgattcga gcctgagccc tggtacctca
     2221 gctcgcggat cgcagcgccc gttgccgcgc tccagatccg gacggtgcca tctcccccgg
     2281 cggtgatgat ccgggcgccg tccgggctga acaccgcccc atacaccgta tcgccatggt
     2341 tcatggtgaa cagcagctgg cccgacgacg cgtcccacat ccgagcgctc ttatcgtcgg
     2401 tcgtgagcac ccgcttgcca tcgggcgcga acgcggccga ccacacgcgt ccagcattgc
     2461 tcgcgaagcg cgcgagctcg gacatgtgcg gttgcaacgc acgagcgagc ataaatgtca
     2521 cccccgggga gtgctcgccg cgctgatacg cctgctcaag gtgccgcacc gcttcgctcg
     2581 actcactgtg taggagtgct tggcggccct gctcaacatg agattcgatc ccgattcgct
     2641 cgttcatgcg agcctgcatc acccctcggt acagaaacac gccgaccgcc acgagcccgg
     2701 cgagcgctac cagcgctcgc cgcacccaca agcgccggcg agcacgccgc tggctggcga
     2761 cgacgaacga gcactccagc tcgctgagcc cgccctcggc cgcggcgttc gccacaaccc
     2821 caacgtctgc gagcacgtca tcaccccaca acaggcccgg caggtgagac cggtcctccc
     2881 actgctgggc cgcagcccga agttgctcgc ggtcgctcgt gcgcagcacc gcgcgcaggt
     2941 ccgccgccag gtcgagcacg ctgcgctgac gactctctgg gtacttgctc aacgcgcgct
     3001 ggaggacgcg atcgagctcc cttgaaacgc caaaccagag cgatggcgcg gccatgtcga
     3061 cgggcgggac ctcgccatag ccatgcagcc gcctatattc ggtggtgttc tttccggtga
     3121 aaggccgacg ccccgtagct gcctcgtaga tcacgacgcc gagcgagtag atgtcggccg
     3181 ccggaccaac cccggccgcg tcccgccact gctcaggcgc catgtacggt gccgacccga
     3241 acgccgcgcc cctcttggtc agctgctccc tttccttcgc ctctgtcttg ggccggatca
     3301 cgccctctcg cggcgacggc cggatcaacg acgtcggcac ccgatccgct tcgctctcgt
     3361 cgacgagcgt cacatcggga tccacatcgg gatccacctt ggcgattccg aaatcgagca
     3421 gcttggggaa cagccgcccg ttccgctcga tcaccatcat attgctggac ttcagatcgc
     3481 gatggatgat cccgtggttg tgcgcttcat acacgacctc ggctacgtac tcgaagtacg
     3541 gcacgagatg ctccagcggc atcggcccat gcgccttcaa ccagtcggcc aaagtcacgc
     3601 ccctgaccag ctccatcgcg atccaccata ggcgctgacc ctgatcctcg tgcacaccga
     3661 aggcatggac atgcgtcgcg cagggatggc caagctgcga cgcgagtcgc gcctcgcgca
     3721 agaaacgctc caccgatgcc cccttctcga tcctttcccc gtgcaacacc ttcaccacca
     3781 cctcgcggcc gagcggcttc tgctccgctc gatacacgag accccagcca ccctctccga
     3841 tcttcgagtg aataacgaag tcgcccaacg tgcgtccaga aaggtcgaga tcgtgatctg
     3901 ccatcagctc ctcgggggca aagcctagac accacacccg gtacgagggc aatagctcac
     3961 cgtcgtctct tgatgtacca gcgtactccc gcacggggtt ccagatgtag gataatcgtc
     4021 tgccgtatag ttgctcacca aagttccagc ggtagatccc gtgtctccag ctgcgcgcct
     4081 tcgcactcct cgcgcgccgg cgagccgtgg cgatcggcgg caaagttcct cggcgcggtg
     4141 gcggtgtcac gagctcgagt agtggcgatc ggcgtcgagc ccgggagcac cgcgccgggg
     4201 ttcctcgccg cgtgcgcgct cagctgtaga gccccagggc ggatgactga cctcaccacc
     4261 ccccctcaga ggcgcatttc ccgccgcatt ttgcggcatg agcgccatga taccgccccg
     4321 ggagtgtgca aggtgtccgc ggagccgtgg tccaggctgg atttccgaga cacccacgag
     4381 gagccgtttg taagcgacac tgctagcaac accgcgatcc ggcttcgcca gggtcggagc
     4441 gatgtccgcg aagttttggg aggcgagcga tccgcgcgac acccgctgca tcgtccctgg
     4501 aggcgagcga tcgcgcgaca ccccccttgc agcctcctac cgcggagtgt tctctgatcc
     4561 catgggtccg atcgacaagc tctgccaggg tgcgccaact tcgcccgctg cacctagctc
     4621 gttccggctc agctcggtgg cgccgacact gagccagtcc ccctcccccg tgtcccctgt
     4681 gacggcgcgg acaccaccgc ttcccgatga tcggtttgtg ggttgtgacg cggggcccga
     4741 tccgacggtc gcgatcggtg agaacaatgt cgaaggagac tgggagctgc gtctcggcga
     4801 gatcagcatc ggtggatcgc gccagtgcac gatctcgatc ccggaccgag ggatgtcggc
     4861 gcggcactgt ctgctggacc gccgcgcgcg cgcgcgcaag cttctgctgt acgacctcga
     4921 cagctcgcac ggaaccttct tccgggagca aaagctcgaa ggctcggtgg agctgaggcc
     4981 gggcgacatg ttcacggcgc ctccggtaac gtttgtctgt cttagcgaag cgatgcggca
     5041 gcaccgcccc accgtgttcg agatcctggg atcggacgct gcccggccgc cggactgggt
     5101 cgtggtccag gccgcgaccg gctcgggacc gatgctgctc actggcgaag ccggttgtga
     5161 cctcgaccgc ctggcgagtg cgatccacgg gatgacgtcg cgccgactcc ggagtcccat
     5221 cgagcttgcc gccattcccc acgaccacgc ggcacggacg gacctgattc agcaagcctc
     5281 caagacctcg ctgatccttg ccttggacga cgagagcgaa ccgctccctc cggacgtcgt
     5341 gtcgaagctg ttcgatgcaa ggctcggcgt tcgattcatc gccattgcgt cgagtccgaa
     5401 catcgcgcgg cgcgtgctga ccggcgccaa ggtcgagttc atgcaacacg ttccggtgcg
     5461 cccgctcgcg gaccgcaagg acgagatccg cgagctgcta gatcgccagt tcgcgaagcg
     5521 cgggttttgc cggcgggtca acgacctgac gccggtgaac caggacgcgc tcaagacata
     5581 cccgtggccg ggcaacttcg ccgagctacg cgaggttgtt gacgggatca ttgcccatgc
     5641 caagctgggc ggcctacgcc ccgccgcgga gtcgctgggg atctcgcacc agaagctgga
     5701 ccggcgcttt cgacgcgtcg gcctgaaaat ccggcggctg ttcggcgagg gcgagtagcc
     5761 cgacaaatct gtctcacgac gtggggtggt agctcgcaag gcgagacata cagtggagcg
     5821 cttactggta cgtccttcga tacttgaggg ccatcgagct gtctcgcgaa atcgatgcga
     5881 attggcattg cgagaacacc ccttgcaatg catctgattt cgtgttctgt ggtgatgtgc
     5941 tggatcgccc gagacagcgg catgaatgtg cgccgccatc gatctctgat atttcagggt
     6001 cgaccagcgc cgcattcgtg cacaagcgcc tccggatgct ggtccagatt cgtgcactcc
     6061 gagaggcaca cgagcagggt ctcgcgctgg acatggccat cgcggccgat gggctggcga
     6121 ccgctgcgga cgaattggca gcgagtgtcc ggaacctcct cgcgcgcaag gcgcgcatgg
     6181 aaacccatgt gcagcacagc cgcgcctacc gcttgcgaag ggcacacgtg gatgcacagg
     6241 tgcagaacgc actgtacgaa gctcgcgcgc ttcgagagtg gatcgctggc ctgttcgaga
     6301 cgttcttacg accagaacct ccagacccat cgtcagtttg gccggacggg cgctcacttc
     6361 cagagagcta tccgtggctt cggttgccgg agtggattcg ctcccggagg gaagatgagc
     6421 aacatcgccg cgtgctggag gccaaggctg ccgagtgggt gcagagcggg ggtggccagt
     6481 cgcgcctcct ggacgccgac gagctacgcg aggttcgcaa ctggctcagc gacgagaagg
     6541 cgcgcgctcc aggcgtgaac gaggagatgc aaagcctgct cgcccacagc gacgccgcgt
     6601 tggcagcaca ggtcgccgaa gccgaaggcc gccgccgtcg cttgcgacga tgggtgacga
     6661 tcgcccttgt ggttctgacc ggcgcggtct tggcggtgtc gacactggcg gttatcgccg
     6721 tgcgacgatc acgcgtagca gacgagcagt cgcaagaggc gcggcggcag tcacaagagg
     6781 tgcagcggca gtcgcaggag gcacggcggc aactcgccgc tactatgttg ctcgggggta
     6841 ccaactcctg atgaacagcc agcccgcaca ggcagcaccc tatctgctcg cagcccgcaa
     6901 cgccgaggat ggtgccgagg attcaccggg accggcggat cgggcgctgc cgcccctgtg
     6961 tggtcaccag cccccctgtt gacactggcg cgccccgacg aagtgcttcg gagccaggac
     7021 ggcgcggcta ctgggacgca ggtcgcgctg cgccggtcct cagccgcacg agctcgcgcg
     7081 ccgcggcctc gtcgtggggc tcgacccgca gcgcccgctc cagcaagcgt gccgcctcat
     7141 cggggtgacc gcgctcgacc gcacacgccg cgagcacctc taggttctcc ggctcgtagg
     7201 gcagatatcg gtcaaccagc gacgccgcct tgtgcaacac cgtgcagtcg gccttgcctc
     7261 gggcgtcccg caccgccatc agcgcctcgg ctgggaggaa ctggccgtcg gcaagcgcga
     7321 tcacggtctg ggcggagaag cttcgctggt acgcctgcat ggcgagatag cgaggcacca
     7381 gcgcggcgac gcacagcgtc ccgcagatgc cgagggaggc catgccgagg cagggtggca
     7441 cgcggtacga caagcgcttg ccctcgttgc gaagcgcccc cgcgatcacc gcgatcagcg
     7501 cgagcgacgg cggacgatac aggagcgcgt cgaacgcgct gatcacgacc gccacgatca
     7561 ggctcgccgc caacgcgagc ggcacgcgat cgtccgccgc ggcgatccgt gctcgcgcgc
     7621 ccaggagcaa cgccgcgagc atcgacgcgg ccgcgcacag gcctatcacg ccgagctcga
     7681 ccgcctggcg aacgaggtcc gaggcgggtg tccacagcgc gtcgatgaac ccggtgtggt
     7741 atccggttgc ctggtgcacg aaccgcggtg cgtcctgccg ccatgcgccc ggtccgagcc
     7801 cgatcaacgg gcgctccgcg agcatggtga taccgaccag gtgctggtcg acacggctgc
     7861 ggccggtgcc ggtctgggac tcgagcatcc gcgagaacga cgacagaatg ggcgatgact
     7921 ccttccactg gagcccgatc cacggcgtga actggagcgc ggcgaccgcg gcgaggatcg
     7981 cgccgagcgc gaccaaccgc gcgcgtcgcg ccggtcgcgg agcgtcgctc cggggcccgc
     8041 gtcgcttgat ctgccagagg aagtagaggg cgccggtgac catcaggccc atccacgcgc
     8101 tgcgcgcccg gcacaacagg acggacagga gcaggaccgc cagcgcgcca ccgcgcaggt
     8161 aggtcggccg cgtccagagg cggacgatcg cgatcgggat caggatcgcg cagaagccgc
     8221 caaccgcgtt tcggttgccg aacgttgcgc cgggccgacg cccgacgtcc caggggatga
     8281 acccaccggc tgactcgtag atcgtaaggg cagcaagaac caccccgacg acgagcaggc
     8341 cgtcgagcaa ctgcgcgcga tcctgccgcg acgcttcgct tcccaccgcg accatcacga
     8401 gcgcggtgaa gttcagcgcc agccatggcg acaggaacgg gagcgccggg cgattcatcg
     8461 cgaccgagag cgcaccggcg accaggtagc cgagcaccgc gagctcgaac caggtgagcc
     8521 cgagcgttcg tcgtcgccag ctccacgcgc acacgcccgc gcacagcagc gcgcccaccg
     8581 cgagcaccgg cgacttgatg gccaccaacc ccacggcgtg cagcggcgag gctgcgccat
     8641 cgacgctgac cgcgaacgcc gtcgcggcca tcaccgccac ggtggcccag gtcaccacgg
     8701 gccgaacgtg cgcgcgcggc ggtgagtccg gaggcggcgt catgtcggcg cagtcagctt
     8761 ggccagcgcc tccgcgcaca acgccgtcgt gagctcctcg gatccatagt aggtcggctg
     8821 gaagtcgcag aagaacgtcc gcgcgggcca gccgccgtcc gggcgctgcc ggcggagtag
     8881 ccaccgccca gcggcctcga gaccttcccc gcgatggccg gcatcgagca gcgcggtgag
     8941 cgacagcgcg gtgtcgagct cgtgcccgaa gctgccatcg ccggcctggt gtgccagcag
     9001 gtgcggcggg acgcgcgcgg ccaaaggcgc gagcgctggt gccgggccgt ggcagaacgc
     9061 ccgcatcagg gtgtagatcg cgggcggatc ggacagcgcg tacgcgctca gcttcgagag
     9121 ctgacgctgc agcgcgaact cgacgaggaa gcgggcgagc gggccggttg gctggccgga
     9181 caccgccagg tagtacagga tgttcgcgtt gaccaccgcg tccactctcc tgggatcgtt
     9241 gagcccggga tggacccacg tgccgtagcc gcccgacggg aacgcggcgc gcgcgagcag
     9301 cgccaggacc ttggtctcga tcgtcaggcg cgcctggtgc agcgccacca gcgcgcagca
     9361 cgtgtcatcg aagtccggcg gcgggttctg cgcggcacgc ccgaagaacc gccacaggcc
     9421 gcccggctcg ccgtggcccg cgagcgcctg ggcggcggcg gcgagcgcgc tcgtcgtggc
     9481 cggcgtcggg gctgcgtgcg ccag
//
LOCUS       DASVOR010002534         2925 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_144906,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002534 DASVOR010000000
VERSION     DASVOR010002534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 2925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2925
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144906"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1130
                     /gene="aspS"
                     /locus_tag="VF516_46525"
     CDS             <1..1130
                     /gene="aspS"
                     /locus_tag="VF516_46525"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HEX8115294.1"
                     /translation="AAQAESVIAVTGEVCAEPQAPHGVELRDVVIELISEAAEPPPVQ
                     LGKKLANVPLPALLDHAVVANRFPQRRAIFRIGAAAMRGFRAALDAEGFVEIQSPKLV
                     ESATEGGANVFAVDYFGRPAYLAQSPQLYKQVMVGVYERVYEVGPVFRAEPHDTARHI
                     NQYTSLDVELGFLTDHFDVMAQLTRVLRGMLAEVAAACAPELALLGARVPELPATLPH
                     LHFTEALELLAGPLGEAVRGEPDLSPAGERWLGQWAREHHGSDWLFVTGYPMRKRPFY
                     THPDPERPEHSRSFDLLFRGTELVTGGQRLHRHASYLAALEARGMSPDPIAAYLEAFR
                     HGMPPHGGFAIGLERFLLQLLDLPNIRLAVLFPRDLTRLSP"
     gene            complement(1133..1936)
                     /locus_tag="VF516_46530"
     CDS             complement(1133..1936)
                     /locus_tag="VF516_46530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEX8115295.1"
                     /translation="MTDCDRQIRDALSVLDAPRERVQDFLVSDEACVARLVALPEICI
                     GPLLDFARALLRGGPDGTTGEDEMEDREYKLQVILRALFQRTQSRPADPIVSQVGALV
                     IEALLGSTAADKETYVTYLLELGDARAPAALHDFVASTPATDADDGEGWALTKAIEAL
                     RYNRAQQATSSVVQHLDHSAERVRRAAIDFLVEVDASAIAPEFVRRLGLEDDPDNARA
                     MVKALTRWHQIEALPALHRLGASDLARDNAALAAAVHAAINALGDDPNR"
     gene            complement(1933..>2925)
                     /locus_tag="VF516_46535"
     CDS             complement(1933..>2925)
                     /locus_tag="VF516_46535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115296.1"
                     /translation="DSISNIVAGNIGGAANFIEASLAKLVPIAISLFADLIGLGGIAD
                     KIRRIIEKVQTKVDQAIDKLIARVMAMFKGKDKTDGKPNDTPPEERIRRAKQEVEELV
                     AKGVEEDELNTKLEAIKTKYELVALRVEKQGDRVQIHGEINPTFEVMAEEIKYDRDKA
                     AEIAAAGEDHSDEFTTFFRAMSIDELDALRVNKRLMPEMRTPTVPKGMNFITTNADYA
                     KKLMADPKHGKKYEYLVRITVEPGTQAMLEDPSIARIERPNKPTEREFRGKNIRSRGK
                     DERGIVVLKIEPLLQGGGAMNYGITAKTLEGDDPMQMFNARIISIEVIGRMVDP"
BASE COUNT          416 a         1022 c          945 g          542 t
ORIGIN      
        1 gcgcagcgca ggccgagtcg gtgatcgccg tgaccggcga ggtgtgcgcg gagccgcagg
       61 cgccgcacgg cgtcgagctt cgcgacgtcg tgatcgagct gatctccgag gcggcggagc
      121 cgccgccggt gcagctcggc aagaagctgg ccaacgtgcc gctgcccgcg ctgctcgacc
      181 acgccgtcgt cgccaaccgc ttcccgcagc gccgcgcgat cttccggatc ggcgccgccg
      241 cgatgcgcgg gtttcgcgcc gcgctcgacg ccgagggctt cgtcgagatc cagtcgccca
      301 agctggtcga gtccgcgacc gagggcggcg ccaacgtgtt cgcggtcgac tacttcggcc
      361 gcccggcgta cctcgcgcag agcccgcagc tctacaagca ggtcatggtc ggcgtgtacg
      421 agcgcgtgta cgaggtcggc ccggtgttcc gcgccgagcc ccacgacacc gcccgccaca
      481 tcaaccagta caccagcctc gacgtcgagc tcggcttcct caccgatcac ttcgacgtga
      541 tggcccagct cacccgcgtg ctgcgcggca tgctcgccga ggtcgccgcg gcctgcgcgc
      601 ccgagctcgc gctgctcggc gcgcgcgtcc ccgagctccc cgcgaccctc ccgcacctgc
      661 acttcaccga ggcgctcgag ctgctcgccg ggccgctcgg cgaggccgtg cgcggcgagc
      721 ccgacctgtc cccggccggc gagcgctggc tcggccagtg ggcgcgcgag caccacggca
      781 gcgactggct gttcgtcacc ggctacccga tgcgcaagcg gccattctac acccacccgg
      841 atccggaacg ccccgagcac tcgcggtcgt tcgacctcct gttccgcggc accgagctgg
      901 tcaccggcgg ccagcgcctg caccgccacg ccagctacct ggcggcgctc gaggcgcgcg
      961 gcatgtcgcc cgatccgatc gccgcctatc tggaggcgtt ccgccacggc atgccgccgc
     1021 acggcgggtt cgcgatcggc ctcgagcgct tcctgctcca gctgctcgat ctgcccaaca
     1081 tccggctcgc cgtgctgttc ccgcgcgacc tgacccggct gtcgccgtga cgctatcggt
     1141 tcggatcgtc tccgagcgca ttgatcgccg cgtgcactgc cgccgcgagc gcggcattgt
     1201 cgcgcgcgag atcgctggcg ccgagtcgat gcagtgccgg tagcgcttcg atctgatgcc
     1261 agcgggtaag cgccttgacc atcgcgcgcg cattgtcggg atcgtcctcg aggccaagtc
     1321 gacgtacgaa ctctggcgcg atggccgaag catcgacctc cacgaggaag tcgatcgctg
     1381 cgcgccgtac gcgctcggcg gagtggtcga ggtgctggac gacgctcgac gtggcttgct
     1441 gggcccgatt gtagcgcagg gcctcgatcg ccttggtgag cgcccagccc tcaccgtcgt
     1501 ccgcgtcggt cgcgggcgtt gaagccacga agtcgtggag agccgcaggc gctctggcat
     1561 cgccgagttc caagaggtac gtcacgtagg tctccttgtc ggccgccgtg gagccgagga
     1621 gcgcctcgat gacgagcgcg ccgacttgcg agacgatggg atccgcagga cgtgactgtg
     1681 tgcgttggaa tagtgcgcgc aggatcacct gcagtttgta ctcgcgatct tccatctcgt
     1741 cctcgccggt cgtaccgtcg ggaccgccgc gcagcagcgc gcgcgcgaag tcgagaagcg
     1801 gaccgatgca gatctccggc agtgccacca ggcgggcgac gcacgcctcg tcggaaacga
     1861 ggaagtcctg cactcgttcc cgcggtgcgt caaggacgct gagtgcgtct cggatctggc
     1921 gatcacagtc ggtcatggat ccaccatccg tccgatcacc tcgatcgaga tgatgcgtgc
     1981 gttgaacatc tgcatcggat catcaccttc gagcgtcttc gcggtgatgc cgtagttcat
     2041 agcaccgcca ccttgcaaca ggggctcgat cttgagcacg acgataccgc gttcatcctt
     2101 gccccgcgag cggatgttct tgccccggaa ctctcgctcc gtcggcttgt tgggacgctc
     2161 gatgcgcgcg atgctgggat cctcgagcat cgcctgcgtc ccgggttcga ccgtgatccg
     2221 cacgagatac tcgtacttct tgccgtgctt gggatcagcc atcagcttct tcgcgtagtc
     2281 ggcgttggtc gtaatgaagt tcataccctt gggaaccgtc ggtgtccgca tctcgggcat
     2341 cagccgcttg ttcacgcgca aggcatcgag ctcatcgatg ctcatggcgc ggaagaacgt
     2401 cgtgaactca tcggagtgat cttcgccggc tgcggcgatc tccgcagcct tgtcgcggtc
     2461 gtacttgatc tcctccgcca tcacctcgaa cgtcgggttg atctcgccgt ggatctggac
     2521 acggtcgccc tgcttttcga ctcgtagagc taccagctcg tacttggtct tgatcgcctc
     2581 gagcttcgtg ttgagctcat cctcctcgac acccttggcg accaactcct cgacctcctg
     2641 cttcgcccgg cggatgcgtt cctcgggcgg cgtatcgttg ggtttgccgt ctgtcttgtc
     2701 cttgcccttg aacatcgcca tgacgcgggc gatcagcttg tcgatcgcct ggtcgacctt
     2761 ggtctggacc ttctcgatga tgcggcggat cttgtcggcg atgccgccca ggccgatcag
     2821 gtcggcgaac aggctgatcg cgatcgggac cagcttggcg agcgaggcct cgatgaagtt
     2881 cgccgcgccg ccgatgttgc cggcgacgat gttggagatc gagtc
//
LOCUS       DASVOR010002535         7264 bp    DNA     linear   ENV 20-FEB-2024
DEFINITION  MAG TPA_asm: Kofleriaceae bacterium isolate SMAG_U3158 k141_496065,
            whole genome shotgun sequence.
ACCESSION   DASVOR010002535 DASVOR010000000
VERSION     DASVOR010002535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37959786
            Sequence Read Archive: SRR10695399
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Kofleriaceae bacterium (soil metagenome)
  ORGANISM  Kofleriaceae bacterium
            Bacteria; Myxococcota; Polyangia; Haliangiales; Kofleriaceae.
REFERENCE   1  (bases 1 to 7264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/12/2024 01:21:17
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 9,638
            CDSs (total)                      :: 9,594
            Genes (coding)                    :: 9,556
            CDSs (with protein)               :: 9,556
            Genes (RNA)                       :: 44
            tRNAs                             :: 41
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 38
            CDSs (without protein)            :: 38
            Pseudo Genes (ambiguous residues) :: 0 of 38
            Pseudo Genes (frameshifted)       :: 7 of 38
            Pseudo Genes (incomplete)         :: 30 of 38
            Pseudo Genes (internal stop)      :: 4 of 38
            Pseudo Genes (multiple problems)  :: 3 of 38
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7264
                     /organism="Kofleriaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_496065"
                     /isolate="SMAG_U3158"
                     /isolation_source="soil"
                     /db_xref="taxon:2212474"
                     /environmental_sample
                     /country="China"
                     /lat_lon="23.5823 N 104.3303 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /locus_tag="VF516_46540"
     CDS             <1..1006
                     /locus_tag="VF516_46540"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEX8115297.1"
                     /translation="NFGWVGVQLFFALSGFLITGILLDSRDAPHYYRGFFGRRVLRIF
                     PLYYGVLVIALWILPALGLAPPRLLADREHRIWLWTYLVNWTEPFGAGVAAFPHFWSL
                     AVEEQFYLVWPFVVRRATPRRVLHIAAALAVVAFASRLGLLLAGFGEAGPYMFTICRM
                     DALGLGAAVAAVIRIPALHARVVDRRTGILAGAGAVFVVGLLATRGYARTSFVGQTLG
                     YTILAIAFAALVLAAVLDDERGRGRIGAALQNPALRSFGKYSYAIYLFHQPLNQMIGE
                     PVLRSLEPHGVGLAAGLAYIAVVTAASYALALASYHGYEKHFLALKRRFPVTPGAART
                     "
     gene            complement(981..2051)
                     /locus_tag="VF516_46545"
     CDS             complement(981..2051)
                     /locus_tag="VF516_46545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEX8115298.1"
                     /translation="MTGDLVSVVIPSYNRAYCVAETIDSALAQTHGNLEILLVDDGSS
                     DGTRALIAERYRDEPRVRYIHQANAGVSAARNHGLRLARGQYVALLDSDDIWLPWKVE
                     AQLRCLEAFPSAGMIWTDMDAIGPDGELKYHRYLTRMYTSYRKFTREQLFRESRPLSS
                     IDARLAAALDDPRVYLGDIFSPMITGNLVHTSTVLLRRERFEHVREFDVNLRYSGEDH
                     DFHLRTCREGDVAYLDVASILYQYGREDQLTVNPAYTVHMAENFLKTVNKALARDRGR
                     VTLSPAMLDSVMAEAHAWIGECRLQLGERDQAQRHLGTSLKFRPWQPRVAALFGASFL
                     PKRVSDRIRASYRAVKSGLRRA"
     gene            complement(2405..2824)
                     /locus_tag="VF516_46550"
     CDS             complement(2405..2824)
                     /locus_tag="VF516_46550"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEX8115299.1"
                     /translation="MEGDLSLLSIPDLLQMICLGRFNRNIHLFDGPILIGMIAIRDGR
                     IEQCIGLGSRGEAAFYKLVGLRRGRYKIHEATDLTTSDAELSGSSWRMLLLEAARRHD
                     EARHDARSGPAVAMTFDVPTRQTSGSPTLPAALPDFD"
     gene            3386..3550
                     /locus_tag="VF516_46555"
     CDS             3386..3550
                     /locus_tag="VF516_46555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115300.1"
                     /translation="MYLEATLIDNRTGLVVWHAHQTFPAQLDHADDTERVMRTLLAQL
                     PPRAALQARR"
     gene            complement(3618..3746)
                     /locus_tag="VF516_46560"
     CDS             complement(3618..3746)
                     /locus_tag="VF516_46560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115301.1"
                     /translation="MKLWCGRRGFDARLRGSSSVGLVIAFAFAVGLERPQRDDRTT"
     gene            4165..6354
                     /locus_tag="VF516_46565"
     CDS             4165..6354
                     /locus_tag="VF516_46565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEX8115302.1"
                     /translation="MTTQLNLTVPLDGTPDKPLDTSAYAFDGSGALLQSAPLDAGKAK
                     LTVPDTAVARMRVFVGPTLPKDRASKPTADILEKLGGYELVISKRVPGSLDLSLQPIS
                     SDYWKRWFLCRCRVVGRIVKPVSIGGVMTDLPVCHAKVKIFEVDPFWFWLKRLPDPDL
                     FKLRSQLAAELAPPHLPVPGPDPRVTAAAEGLPTLTRAQFPLEQKLALGSDSAIVVRK
                     ALLANPALLRPLFCRSIWSWLTPWLRTTLITTVETDNQGRFESYIWHPCVDQPDLYFN
                     VDYMIDGTLTTVYHRPVACDTHWDYACGSDVVIRVTDPRVLPCAPVSTPGLSIDVKRV
                     GSTGYLHRLRSTGAPQGLIAWNGTLDPRAQETGQTPVPFGGNLYLYSDFSQGLRAGTV
                     ATHYLWSYRRIPVGGTPGGWKPILTPVVRNYVVHPTAGPDRTGVYSLAETVSSGTYFH
                     IQPNDVPSTLYNAGETAEWTVLNEKIDLATASLDTTTAETGGTALGLFDDSSGPVQGL
                     YEFKLELFKKNSSGGMDLVNVDTAGITINVWRLTGHPADPVDFVIAGNDFRFTADTTP
                     IKTAFRMPIRLDNNLLEATITPASVPSASAGPCGFIDYHPGVHVTLGFHAFQRNTWGT
                     YSFAVSRGSAGTLGWPVSINSARVGTVPPPSPSPSPYTSSGSGTFSGPFTINTLMAQI
                     GSGPPNACTDGRAAFAENLSVVAMITNGEDWTQRISAQPLAFALNPI"
     gene            complement(6454..6600)
                     /locus_tag="VF516_46570"
     CDS             complement(6454..6600)
                     /locus_tag="VF516_46570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I lanthipeptide"
                     /protein_id="HEX8115303.1"
                     /translation="MNKHHTTKLDPSTKKLALRKELIRELQIKELEQVAGGFKTTEKS
                     TGCC"
     gene            complement(6744..6959)
                     /locus_tag="VF516_46575"
     CDS             complement(6744..6959)
                     /locus_tag="VF516_46575"
                     /inference="COORDINATES: protein motif:HMM:NF025198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HEX8115304.1"
                     /translation="MGRFRAGYCGLIGLLVPLVGPIGLILGIYALRDLKQHPEKRGRG
                     RAITGIILGGLGTALWLLWLVTVVVRH"
BASE COUNT         1116 a         2616 c         2371 g         1161 t
ORIGIN      
        1 caacttcggc tgggtcggcg tccagctgtt cttcgcgctg tccgggttcc tgatcaccgg
       61 gatcctgctc gacagccgcg acgcccccca ctactaccgc ggcttcttcg gccgccgcgt
      121 gctgcggatc ttcccgctgt actacggcgt gctcgtgatc gcgctgtgga tcctccccgc
      181 cctggggctc gcgccgccgc ggctgctcgc cgaccgcgag caccggatct ggctgtggac
      241 ctacctcgtc aactggaccg agccgttcgg cgccggggtc gcggcgttcc cgcacttctg
      301 gtcgctcgcg gtcgaggagc agttctacct ggtgtggccg ttcgtggtcc gccgcgcgac
      361 gccgcgccgc gtgctgcaca tcgccgccgc gctcgcggtg gtcgcgttcg cgtcgcggct
      421 cggcctgctg ctcgccgggt tcggcgaggc cggaccgtac atgttcacga tctgccggat
      481 ggatgcgctc gggctcggcg ccgcggtcgc cgcggtgatc cggatccccg cgctgcacgc
      541 gcgcgtcgtc gaccgccgca ccggcatcct ggccggcgcg ggcgcggtgt tcgtcgtcgg
      601 tctgctcgcg acccgcggct acgcgcggac cagcttcgtc ggccagaccc tcggctacac
      661 gatcctcgcg atcgcgttcg cggcgctcgt gctcgcggcg gtgctcgacg acgagcgcgg
      721 ccgcggccgg atcggggccg cgctgcagaa cccggcgctg cgctcgttcg gcaagtacag
      781 ctacgcgatc tacctgttcc accagccgct caaccagatg atcggagagc cggtgctgcg
      841 ctcgctcgag ccccacggcg tcgggctcgc cgccggcctg gcctacatcg cggtggtgac
      901 cgccgcgagc tacgcgctgg cgctggcctc gtaccacggg tacgagaagc acttcctggc
      961 gctcaagcgg cggttccccg tcacgcccgg cgcagcccgg acttgaccgc gcgatagctc
     1021 gcccggatcc ggtccgagac ccgcttgggc aggaacgacg cgccgaacag cgccgcgacc
     1081 cgcggctgcc acggccggaa cttcaggctg gtcccgaggt ggcgctgcgc ctgatcgcgc
     1141 tcgccgagct gcagccggca ctcgccgatc cacgcgtggg cctccgccat cacgctgtcg
     1201 agcatcgcgg gcgacagcgt cacccgaccg cgatcgcggg ccagcgcctt gttcaccgtc
     1261 ttgaggaagt tctcggccat gtgcacggtg taggcggggt tcaccgtcag ctggtcctcg
     1321 cggccgtact ggtagaggat cgaggcgacg tcgaggtacg cgacatcgcc ctcgcggcag
     1381 gtccggaggt ggaagtcgtg gtcctcgccc gagtagcgca ggttgacgtc gaactcgcgc
     1441 acgtgctcga accgctcgcg gcggagcagc accgtcgagg tgtgcaccag gttgccggtg
     1501 atcatcggcg agaagatgtc gccgaggtac acccgcggat cgtccagcgc ggccgccagc
     1561 ctggcgtcga tcgacgacag cgggcgcgat tcgcggaaca gctgctcgcg cgtgaacttg
     1621 cggtacgagg tgtacatccg cgtcaggtac cggtggtact tgagctctcc gtccggcccg
     1681 atcgcgtcca tgtcggtcca gatcatcccg gcgctcggaa acgcctccag gcagcgcagc
     1741 tgggcctcga ccttccacgg cagccagatg tcgtcggagt cgagcagcgc gacgtactgc
     1801 ccgcgcgcca gccgcagccc atggttccgc gccgccgaca ccccggcgtt ggcctggtgg
     1861 atgtagcgca ccctcggctc gtcgcggtag cgctccgcga tcagcgcgcg cgtgccgtcg
     1921 gacgagccgt cgtcgacgag caggatctcc aggttgccgt gggtctgggc cagggcgcta
     1981 tcgatggtct ccgcgacgca gtacgcgcgg ttgtacgacg ggatcacgac gctgacgagg
     2041 tctccggtca tgcttacatc ccttgtccgt tcgccgcggc gggccgcttg ctccgatcta
     2101 gctcggacat tcgcaccatc atccgacgaa ccagtcgttg ccgccggctt ccgaagcccg
     2161 gcctcacgct cgcctccggc gcggcgctgc tcgcaccagg gtggccaccc cgcgagctcg
     2221 gcgccacgca cgaccgaggg ccgaaccacg gaccccggga ctgcactgca tggacggaag
     2281 gtaccagaat ctcgcaggcc gcggcgcgac ccgaccggcc ggcgtggctc acccgcacgc
     2341 accccctgcc tcgccgcgcg ggacggtacc tccgtgcgga gcgcgcgtcc tgccgtcaga
     2401 gaatctaatc gaagtcgggc agcgcagcgg gcagcgtcgg cgatcccgac gtctgccggg
     2461 tcgggacatc gaacgtcatc gcgaccgccg gccccgagcg cgcgtcgtgc cgggcctcat
     2521 cgtggcgacg cgcggcctcc agcaggagca tccgccagga cgacccgctc agctccgcgt
     
[document cut: first 32166976 of 33275190 characters kept]
